bluuebunny/crossref_metadata_2025_split huggingface dataset:
A robust eleven-genes prognostic model can predict overall survival in bladder cancer patients based on five cohorts
Authors: Lin Jiaxing , Yang Jieping , Xu Xiao , Wang Yutao , Yu Meng , Zhu Yuyan 
Published: July 2020

Abstract
Background: Bladder cancer is the tenth most common cancer globally, but existing biomarkers and prognostic models are limited. Method: In this study, we used four bladder cancer cohorts from The Cancer Genome Atlas and Gene Expression Omnibus databases to perform univariate Cox regression analysis to identify common prognostic genes. We used the least absolute shrinkage and selection operator regression to construct a prognostic Cox model. Kaplan-Meier analysis, receiver operating characteristic curve, and univariate / multivariate Cox analysis were used to evaluate the prognostic model for the four cohorts. Finally, a co-expression network, CIBERSORT, and ESTIMATE algorithm were used to explore the mechanism related to the model. Results: A total of 11 genes were identified from the four cohorts to construct the prognostic model, including eight risk genes (SERPINE2, PRR11, DSEL, DNM1, COMP, ELOVL4, RTKN, and MAPK12) and three protective genes (FABP6, C16orf74, and TNK1). The 11-genes model could stratify the risk of patients in all five cohorts, and the prognosis was worse in the group with a high-risk score. The area under the curve values of the five cohorts in the first year are all greater than 0.65. Furthermore, this model's predictive ability is stronger than that of age, gender, grade, and T stage. Through the weighted co-expression network analysis, the gene module related to the model was found, and the key genes in this module were mainly enriched in the tumor microenvironment. B cell memory showed low infiltration in high-risk patients. Furthermore, in the case of low B cell memory infiltration and high-risk score, the prognosis of the patients was the worst. Conclusion: The proposed eleven-genes model is a promising biomarker for estimating overall survival in bladder cancer. This model can be used to stratify the risk of bladder cancer patients, which is beneficial to the realization of individualized treatment.
WITHDRAWN: Analysis of the effectiveness of wound closure techniques among surgeons and emergency physicians in Mecca Hospitals: Retrospective Cohort Study.
Authors: Felemban Jeehad Mansour , Binyaseen Kenan , Almalki Waleed , Altowairqi Fahad , Sakhakhini Amer , Alharbi Ziyad 
Published: July 2020

Abstract
Objective: Wound suturing is a procedure performed at many medical specialties. This study aims to analyze the effectiveness of wound closure techniques by recognizing the common suturing techniques and materials used in different types of wounds among surgeons and emergency physicians in Mecca hospitals and assessing their awareness about the factors contributing to scars formation and improper wound healing.Result: Choosing the simple interrupted technique exceeded 50% among all other types of studied wounds. The other most common techniques among participants were the subcuticular and vertical mattress, respectively, also using stapler was limited to some wound types. This study showed a high level of awareness among participants with significant p-values (P<0.05) of Strongly agree and Agree regarding the essential factors affecting wound healing and scars formation. The result gives an insight into the most common suture techniques and materials used in wound closure. There are wide variations in selecting these techniques and materials among the participants. Also, it showed that there is good awareness among participants about factors that lead to scar formation.
The prognostic link between metabolic syndrome and the different breast cancer molecular subtypes: a case control study.
Authors: Chen Yi zuo , Zhou Yu ying , Chen En dong , Hu Di shuang , Shao Xuan , Cai Ye Feng , You Jie 
Published: July 2020

Abstract
Background Metabolic syndrome (MetS) is an important factor related to the poor prognosis of breast cancer (BC). Molecular heterogeneity in the tumor may affect the consequence of BC. The main purpose of this study was to assess the prognostic link between MetS and the different BC molecular subtypes.

Methods A total of 960 patients with BC were recruited from January 2010 to June 2014. The relationship between MetS and disease prognosis was assessed by using univariate and multivariate analyses.

Results At recruitment, MetS was diagnosed in 199 patients (20.7%). The mean follow-up period was 68.5 months (range, 2–103 months). MetS remained significantly associated with 64% increased risk of recurrence (Hazard Ratio (HR)=1.64; 95% confidence interval (CI) 1.19–2.27, P<0.01) and twofold increased risk of mortality (HR=2.02, 95% CI 1.29–3.16, P<0.01) according to multivariate analysis. By reviewing BC molecular subtypes, the significant associations remained in the subsets of Luminal A (HR=3.1, 95% CI 1.28–4.57, P=0.01), Luminal B (Her2-negative) (HR=3.3, 95% CI 1.31–8.33, P=0.01), and Her-2-positive (non-Luminal) (HR=2.2, 95% CI 1.10–4.39, P=0.03).

Conclusions MetS was significantly related to unfavorable prognosis in operable BC, especially in the subgroup of Luminal A. More studies are needed to definitively determine which factors influence the association of MetS with Luminal A subtype.
The stem cell transcription factor SOX2 is essential for astrocyte maturation and controls animal hyperactive behavior
Authors: Zhang Sheng , Lan Zhaohui , Wang Yan , Kim Bokyung , Guo Fuzheng 
Published: July 2020

Abstract
Children with SOX2-deficiency develop anophthalmia/microphthalmia and display neurological impairment. Here we report an essential role for astroglial SOX2 in brain anomalies and neurological defects. Sox2-deficiency inhibited postnatal astrocyte maturation without affecting astroglial proliferation and population expansion. Mechanistically, we found that SOX2 directly bound to a cohort of astrocytic signature genes such as those involving in glutamate transport and that Sox2-deficiency remarkably reduced glutamate transporter expression and compromised astrocyte function of glutamate uptake. Behaviorally, astroglial Sox2-deficient mice developed hyperactivity in locomotion while their motor skills, social capability, and learning abilities were unaffected. We found that astroglial Sox2-deficiency results in elevated glutamatergic synapse formation and elevated excitability of striatal medium spiny neurons, which has been shown to trigger hyperactive locomotion. Our study provides new insights into the biological mechanisms underlying brain defects in children with SOX2 mutations and unveils a novel connection between astrocyte SOX2 and brain development and behavior.
Therapeutic activity of sarpogrelate, and dopamine D2 receptor agonists on cardiovascular and renal systems in alloxan-induced diabetic rats
Authors: Fouad Mohamed , Latif Hekma A. Abd El , Galal May , Yamani Mohammed El , Hassaneen Sherifa , Masoaad Raneem 
Published: July 2020

Abstract
Background Dopamine D2 agonists are notable medications in the treatment of Parkinsonism, hyperprolactinemia, and hyperglycemia. An affiliation showed between the enlistment of myocardial injury ailment and long haul treatment with dopamine D2 agonist drugs identified with the partial initiation of 5-HT 2a receptors.Methods The investigation aims to represent the activity of sarpogrelate, a particular 5-HT (2A) receptor blocker, in diminishing myocardial injury prompted by extended haul utilization of D2 agonist drugs in diabetic rodents. Both bromocriptine and cabergoline managed independently and combined with sarpogelate for about a month to diabetic nephropathy rats.Results Bromocriptine and cabergoline created a significant reduction in BGL, BP, and kidney hypertrophy index in diabetic nephropathy rats. Administration of bromocriptine, cabergoline, alone, or in combination with sarbogrelate fundamentally diminished blood concentrations of ALP, AST, urea, and creatinine. Bromocriptine and cabergoline alone showed noteworthy ascending of LDH-1, Troponin I, and TNFα1 levels in the serum (p < 0.05). Paradoxically, utilizing bromocriptine and cabergoline with sarpogrelate treatment altogether diminished the degree of the myocardial biomarkers in the serum. A mix of bromocriptine or cabergoline with sarpogrelate diminished the level of the myocardial infarct size in the heart assessed utilizing the TTC staining method.Conclusions The examination exhibited that both bromocriptine and cabergoline could be utilized securely in blend with sarpogrelate for a long duration of treatment for diseases like hypertension and diabetes.
Relationship Between Integrin β1 (ITGB1), β3 (ITGB3) and Intracerebral Hemorrhage (ICH)
Authors: Ma Hai-Yang , Xu Yan , Qiao Chun-You , Peng Yi , Ding Qi , Wang Li-Zhong , Yan Jun-Fei , Hou Yuan , Di Fei 
Published: July 2020

Abstract
Background: Intracerebral hemorrhage (ICH) is fatal and detrimental to quality of life. Clinically, options for monitoring are often limited, potentially missing subtle neurological changes especially in low-grade patients. This study explored the relationship between expression of integrin β1 (ITGB1), β3 (ITGB3) in intracerebral hemorrhage (ICH) to analyze their functional and clinical relevance. Methods: The expression of ITGB1 and ITGB3 in ICH was accomplished by immunohistochemical (IHC) staining and western blotting (WB) analysis, respectively. Results: Furthermore, the results demonstrated that TGB1 was expressed in ICH tissues, but ITGB3 was not expressed in ICH tissues.Conclusions: In summary, the clinical progression of ICH was related to the expression of ITGB1. ITGB1 may be a potential biomarker and contribute to the treatment of ICH.
Targeted Protein Liposomes-Mediated AChE Gene Therapy for Effective Liver Cancer Treatment
Authors: Wang Kai , Shang Fusheng , Chen Dagui , Jiao Jianpeng , Cao Tieliu , Wang Xiaowei , He Shengli , Liang Xiaofei 
Published: July 2020

Abstract
The development of highly efficient non-viral gene vector systems has very important application value in the field of cancer therapy. The high protein content of proteolipids allows for high biocompatibility, low immunogenicity, and surface modification of proteins to confer more targeted drug/gene function. For the first time, this study selected transferrin, which has hepatocellular carcinoma cell targeting function, with a liposome backbone material to construct transferrin liposome (Tf-PL), and load acetylcholinesterase (AChE) therapeutic gene for in vitro and in vivo functions evaluation. The results showed that the Tf-PL transfection efficiency was higher than that of commercial Lipo 2000, low cytotoxicity and targeted ability to liver cancer SMMC-7721 cells. After tail vein injection, Tf-PL/AChE can effectively target to liver cancer, significantly inhibiting the growth of liver cancer xenografts in nude mice, prolonging the survival time of tumor-bearing nude mice, and also does not cause significant systemic toxicities. Our study provides a strategy for proteolipids targeting the transferrin receptor to carry therapeutic gene therapy for tumors. This method has strong tumor affinity and can provide an effective vector selection for precise tumor therapy.
Patterns of Informal Family Care During Acute Hospitalization of Older Adults from Different Ethno-Cultural Groups in Israel.
Authors: Shulyaev Ksenya , Gur-Yaish Nurit , Shadmi Efrat , Zisberg Anna 
Published: July 2020

Abstract
Introduction Informal caregiving during hospitalization of older adults is significantly related to hospital processes and patient outcomes. Studies in the home settings demonstrate that ethno-cultural background is related to various aspects of informal caregiving; however, this association in the hospital setting was insufficiently researched.Objectives Our study looked for differences between ethno-cultural groups in the amount and kind of informal support they provided for older adults during hospitalization.Methods This study is a secondary data analysis of two cohort studies conducted in Israeli hospitals. Hospitalized older adults were divided into three groups: Israeli-born and veteran immigrant Jews, Arabs, and Jewish immigrants from the Former Soviet Union (FSU).Results Informal caregivers of “FSU immigrants” stayed less hours during the day in both cohorts, were less present during the night in study 1, and provided less supervision of medical care in study 2, in comparison to the two other groups. Findings from study 1 also suggested that informal caregivers of “Arab” older adults were more likely to stay during the night in comparison to the two other groups.Conclusion Ethno cultural groups differ in terms of the patterns of caregiving older adults during hospitalization. Health care professionals should be aware of these patterns and the cultural norms that are related to caregiving practices for better cooperation between informal and formal caregivers of older adults.
WITHDRAWN: IoT-based Machinery Failure Predictive Solution using Big Data Analysis on the MIMII Dataset
Authors: Talmoudi Sana , Kanada Tetsuya , Hirata Yasuhisa 
Published: July 2020

Abstract
One of the main focuses of smart industry is machinery failure predictive solutions. To achieve this, IoT-based solutions have been widely deployed. However, data processing and decision making remain challenging. The absence of enough knowledge has been the primarily limitation of statistical methods and supervised learning methods. Therefore, unsupervised learning methods are gaining more popularity but still have limits to cover effectively the pre-signs of failures due to the complexity of training process and results visualization. Previously, we proposed a novel Big Data Analysis method on audio/vibration data to cover effectively the pre-signs of failures through data visualization without complex learning or processing. We validated our proposal on a demo system. In the present work, we are using part of the MIMII dataset to test our proposed analysis method on a real-world-like data and verify the validity of our proposal on a more complex system. We are showing that we can detect abnormal machine behaviors and predict failures without prior training or knowledge of the target monitored machine.
Study on the Protective Effect and the Metabolomics Features of Rutaecarpine on Ethanol-induced Acute Gastric Ulcer
Authors: ren sichen , Wei Ying , Niu Ming , Wang Ruilin , Wei Shizhang , Wen Jianxia , Wang Dan , Yang Tao , Chen Xing , Wu Shihua , Tong Yuling , Jing Manyi , Li Haotian , Wang Min , Zhao Yanling 
Published: July 2020

Abstract
As a prevalent digestive disease, Gastric ulcer (GU) has a high incidence and is seriously harmful to human health. It is an urgent need to finding a natural drug with a gastroprotective effect. Rutaecarpine (RUT) is an alkaloid isolated from Evodia rutaecarpa Bentham (Rutaceae). This plant is a traditional Chinese medicine that has been treating gastrointestinal diseases for a long time. The present study aimed to investigate the effects of RUT on ethanol-induced acute GU in mice. After intragastrical administration of RUT for 3 consecutive days, acute GU were induced in the mice by ethanol treatment for 1.5 h. The expression levels of SOD, CAT, NO, ET-1 in serum and EGF, TNF-α, IL-1β, IL-6 in gastric tissues were measured by kits. In addition, hematoxylin and eosin (H&E) staining of gastric mucosa for pathological changes was evaluated. The serum metabolomics method based on ultra-high-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was used to explore the potential mechanism. A total of 7 potential metabolites involved in 9 metabolic pathways were identified. This study helps us to further understand the pathogenesis of GU and provide a potential natural anti-ulcer drug for clinic.
Cytoplasm Protein GFAP Magnetic Beads Construction and Application as Cell Separation Target for Brain Tumors
Authors: Zhao Yang , Jiang Feng , Wang Qinhua , Wang Baocheng , Han Yipeng , Yang Jian , Wang Jiajia , Wang Kai , Ao Junping , Guo Xunxiang , Liang Xiaofei , Ma Jie 
Published: July 2020

Abstract
BACKGROUND: It is very important to develop a highly efficient cerebrospinal fluid (CSF) detection system with diagnosis and prediction function, for which the detection of circulating tumor cells (CTCs) in CSF is a good choice. In contrast to the past use of epithelial EpCAM as CTCs separation target, a cytoplasm protein of GFAP antibody was first selected to construct highly-sensitive immunomagnetic liposome beads (IMLs). The validation and efficiency of this system in capturing CTCs for brain tumors were measured both in vitro and in vivo. The associations between the numbers of CTCs in patients with their clinical characteristics were further analyzed. RESULTS: Our data show that CTCs can be successfully isolated from CSF and blood samples from 32 children with brain tumors. The numbers of CTCs in CSF were significantly higher than those in blood. The level of CTCs in CSF was related to the type and location of the tumor rather than its stage. The higher the CTCs number is, the more possibly the patient will suffer from poor prognosis. Genetic testing in GFAP CTC-DNA by sanger sequencing, q-PCR and NGS methods indicated that the isolated CTCs (GFAP+/EGFR+) are the related tumor cell. For example, the high expression of NPR3 gene in CSF CTCs was consistent with that of tumor tissue.CONCLUSIONS: The results indicated that GFAP-IML CTCs isolation system, combined with an EGFR immunofluorescence assay of antitumor marker, can serve as a brand-new method for the identification of CTCs for brain tumors. Via lumbar puncture, a minimally invasive procedure, this technique may play a significant role in the clinical diagnosis and drug evaluation of brain tumors.
Clinical And Laboratory Characteristics And Growth Outcomes Of Children With Growing Pains
Authors: Liao Chung-Yuan , Lee Jyh-Hong , Wang Li-Chieh , Wu Kuan-Wen , Lin Yu-Tsan , Yang Yao-Hsu , Chiang Bor-Luen , Yu Hsin-Hui 
Published: July 2020

Abstract
Background: Growing pains is a benign, non-inflammatory pain syndrome of childhood, characterized by bilateral recurrent leg pain. Studies regarding the clinical and laboratory proﬁle of growing pains are limited, especially in East Asia. The aim of this study was to analyze the clinical features, laboratory ﬁndings, and follow-up of children with growing pains.Methods: Patients under 18 years of age with growing pains in National Taiwan University Children’s Hospital between April 2006 and April 2019 were enrolled. Clinical features, laboratory data and medication records were analyzed. Changes of body height and weight before and after the symptoms were assessed by the paired samples t-test.Results: After excluding 31 children with musculoskeletal diseases, 276 patients with a final diagnosis of growing pains were included, comprising 152 boys (55%) and 124 girls (45%). The mean age was 4.9 ± 2.7 years. Bilateral and lower limbs pain were present in 232 (84%) and all cases, respectively. There were 6 out of 16 patients (38%) with elevated alkaline phosphatase, and 3 out of 50 (6%) with positive antinuclear antibodies in low titers. There were no significant changes in body height and weight percentiles or z-scores at 0.5, 1, and 2 years after diagnosis, compared with baseline values. Symptomatic treatments were used in 33% of patients.Conclusions: Growing pains is a common benign leg pain syndrome in children. Alkaline phosphatase elevation is commonly detected. It is not associated with rapid changes of body height and weight within 2 years of experiencing growing pains.
Oral Antioxidants Mitigate Levels of Malonedialdehyde and Protein Carbonyl and Improve Semen Parameters in Men with Oligoasthenozoospermia
Authors: Zhaku Vegim , Beadini Sheqibe , Beadini Nexhbedin , Amzai Gazmend 
Published: July 2020

Abstract
Raising awareness about treating options in North Macedonia, while sperm parameters are decreasing because of increased oxidative stress, in a terrain like this, represents a big challenge. All the patients that fulfilled the required criteria, 37 were enrolled in the study signing a document that explains nature of the study. The first semen sample was collected with at least three days of abstinence. From the sample 0.5 ml was used for standard semen analysis, 1.2 ml was used to evaluate the levels of malonedialdehyde (MDA) and protein carbonyl (PC). The last sample was collected after 6 months. Mean, Standard Deviation, the Pearson Correlation and an independent student t-test were used for statistical analyses. Concentration and motility were significantly increased after 6 months of treatment (p<0.001).The level of MDA shows significantly lower values after six months of therapy with antioxidants (p<0.001).Whereas another marker which is denoted by PC was also lower after the treatment, but was not statistically significant (p=0.0554). There is, however, lack of agreement, because improvement is not consistent and there is wide variation in the treatment regimens, on the dose and duration of treatment and whether mono or combined oral antioxidants should be administered. Always keep on mind that, antioxidants are not free from potential side effects “antioxidant paradox”.
Effects of Intravenous Lidocaine on Alfentanil Consumption During Procedural Sedation for Colonoscopy in Patients With Inflammatory Bowel Disease: a Randomized Controlled Trial
Authors: Aalbers Twan , Heuvel Sandra A.S. van den , Bronkhorst Ewald M. , Esch Aura A.J. van , Scheffer Gert Jan , Vaneker Michiel 
Published: July 2020

Abstract
Background: Procedural sedation and analgesia (PSA) is used during colonoscopy to facilitate the procedure and relieve patient’s discomfort. The foremost risk of PSA is respiratory depression. Lidocaine could be a promising additional analgesic in IBD patients to minimise side effects of PSA.Our primary objective was to investigate whether i.v. lidocaine reduces the amount of alfentanil used during PSA in IBD patients. Additionally, we investigated whether lidocaine reduces cardiorespiratory incidents and the amount of propofol required during the procedure.Methods: A randomised, double-blind, placebo controlled study was performed at the endoscopy unit of the Radboud University Medical Centre from November 2016 to December 2018. Seventy-six patients with IBD, ASA 1 or 2, between 18 and 65 years, scheduled for colonoscopy with PSA were included. Patients received lidocaine 1.5 mg kg-1 followed by a continuous infusion of 2 mg kg-1 h-1 (intervention group, n=38) or 0.9% saline in equivalent volumes (control group, n=38) during colonoscopy.Results: There was a reduction in the use of alfentanil (327µg (95%CI=-31-505, p=0.082)), and propofol (39 mg (95%CI=-5-83, p=0.083)) in the lidocaine group compared with the control group. Ten patients (26%) in the control group and 8 patients in the lidocaine group (21%) experienced a period of hypoxia (p=0.788). In both groups, no periods of hypotension were noted.Conclusion: Our investigation has shown a reduction in the use of propofol and alfentanil in patients undergoing colonoscopy with PSA. The differences were not statistically significant. Lidocaine did not reduces the incidence of cardiorespiratory events.Trial registration:EudraCT, 2016-002210-46, registered 19 may 2016,https://www.clinicaltrialsregister.eu/ctr-search/trial/2016-002210-46/resultsISRCTN, ISRCTN47787339, retrospectively registered 6 February 2019, http://www.isrctn.com/ISRCTN47787339