bluuebunny/crossref_metadata_2025_split huggingface dataset:
How enzyme promiscuity and horizontal gene transfer contribute to metabolic innovation
Authors: Glasner Margaret E. , Truong Dat P. , Morse Benjamin C. 
Published: December 2019

Promiscuity is the coincidental ability of an enzyme to catalyze its native reaction and additional reactions that are not biological functions in the same active site. Promiscuity plays a central role in enzyme evolution and is thus a useful property for protein and metabolic engineering. This review examines enzyme evolution holistically, beginning with evaluating biochemical support for four enzyme evolution models. As expected, there is strong biochemical support for the subfunctionalization and innovation–amplification–divergence models, in which promiscuity is a central feature. In many cases, however, enzyme evolution is more complex than the models indicate, suggesting much is yet to be learned about selective pressures on enzyme function. A complete understanding of enzyme evolution must also explain the ability of metabolic networks to integrate new enzyme activities. Hidden within metabolic networks are underground metabolic pathways constructed from promiscuous activities. We discuss efforts to determine the diversity and pervasiveness of underground metabolism. Remarkably, several studies have discovered that some metabolic defects can be repaired via multiple underground routes. In prokaryotes, metabolic innovation is driven by connecting enzymes acquired by horizontal gene transfer (HGT) into the metabolic network. Thus, we end the review by discussing how the combination of promiscuity and HGT contribute to evolution of metabolism in prokaryotes. Future studies investigating the contribution of promiscuity to enzyme and metabolic evolution will need to integrate deeper probes into the influence of evolution on protein biophysics, enzymology, and metabolism with more complex and realistic evolutionary models.Enzymeslactate dehydrogenase (EC 1.1.1.27), malate dehydrogenase (EC 1.1.1.37), OSBS (EC 4.2.1.113), HisA (EC 5.3.1.16), TrpF, PriA (EC 5.3.1.24), R‐mandelonitrile lyase (EC 4.1.2.10), Maleylacetate reductase (EC 1.3.1.32).
Size of nested cities
Authors: Kung Fan‐chin 
Published: December 2019

AbstractModels of cities based on conventional spatial market theory are unable to replicate a realistic size distribution. The stochastic process approach to size distribution, which assumes proportionate growth, does not provide an economic foundation for spatial trades. There is an apparent irreconcilability. We propose that since there is a continuum of equilibria in models of spatial markets with endogenous location, proportionate growth can work as equilibrium selection. We present computations for an urban configuration that has not been presented in the literature before. A small city locates inside a larger city's agricultural supply zone. This generates a larger variation in city size that may include a realistic size distribution.
Cellular mechanism for the improvement of multiple stress tolerance in brewer's yeast by potassium ion supplementation
Authors: Xu Yingchao , Yang Huirong , Brennan Charles S. , Coldea Teodora Emilia , Zhao Haifeng 
Published: December 2019

SummaryThe ethanol fermentation efficiency was affected by multiple stress tolerance of yeast during brewing and bioethanol industry. The effect of KCl on the multiple stress tolerance of yeast cells was examined. Results showed that KCl addition significantly enhanced the tolerance of yeast cells to osmotic and ethanol stress, which correlated with the decreased membrane permeability, the increased intracellular ergosterol and ATP content, and the improved activity of complex II and complex III in yeast cells. Biomass and viability of yeast cells under osmotic and ethanol stress were increased significantly by KCl addition. Supplementation of 4 and 10 g L−1 KCl exhibited the best promotion activity for yeast cells present in medium with 500 g L−1 sucrose and 10% (v v−1) ethanol, respectively. These results suggested that exogenous potassium addition might be an effective strategy to improve yeast tolerance and fermentation efficiency during industrial very‐high‐gravity (VHG) fermentation.
Are maternal mental disorders associated with children's oral health? A systematic review
Authors: Gomes Marcia Andreola Beber , Cademartori Mariana Gonzalez , Goettems Marília Leão , Azevedo Marina Sousa 
Published: December 2019

AbstractMaternal mental problems may lead to a negative effect on children's oral health (COH). The aim of this study was to systematically review the literature to answer the question: Are maternal mental disorders (MMD) associated with COH from zero to 12 years old? Electronic searches were performed in five databases with no language restriction. Cohort, case‐control, and cross‐sectional studies were included. A total of 1183 references were identified, and seven studies were included. In relation to MMD studies investigated: Common Mental Disorders (n = 2), Major Depressive Disorder (MDD) (n = 2), symptoms of depression (SD) (n = 3), Alcohol‐related diagnosis (n = 1), and symptoms of anxiety (n = 2). As outcome, the studies investigated dental caries (n = 5), gingivitis and periodontal diseases (n = 1), sleep bruxism (SB) (n = 1), and malocclusion (n = 1). Positive association was found between MDD and OH (SB and dental caries). There was no MMD associated with malocclusion. Alcohol‐related diagnosis showed a positive association with gingivitis and periodontal diseases and no association with dental caries; anxiety had a positive association with dental caries and no association with SB. The heterogeneity of the data precluded precise conclusions to be drawn, but MMD may have influence on COH.
Instillation negative pressure wound therapy: An effective approach for hardware salvage
Authors: Hehr Jason D. , Hodson Trevor S. , West Julie M. , Schulz Steven A. , Poteet Stephen J. , Chandawarkar Rajiv Y. , Valerio Ian L. 
Published: December 2019

AbstractWound infection involving hardware can be notoriously difficult to treat, often requiring the removal of the infected implant. The goal of this study was to determine the utility of instillation negative pressure wound therapy to help eradicate infection and allow for definitive wound closure in patients without removing the infected hardware. A retrospective review was performed on the outcomes of 28 patients who presented with open wounds with exposed or infected hardware and who were treated with a combination of surgical debridement and negative pressure wound therapy with instillation (NPWTi). Eleven patients were treated for infected spinal hardware, 12 for extremity, and 5 for sternal hardware. Twenty‐five of 28 (89%) patients had successful retention or replacement of hardware, with clearance of infection and healed wounds. Original hardware was maintained in 17 of 28 (61%) patients. In 11 patients, original hardware was removed, with subsequent replacement in eight of those patients after a clean wound was achieved. Average time to definitive closure was 12.6 days. Average follow‐up was 135 days. This series supports NPWTi as an effective adjunct therapy to help expeditiously eradicate hardware infection, allowing for hardware retention.
The Role of Inflation Target Adjustment in Stabilization Policy
Authors: EO YUNJONG , LIE DENNY 
Published: December 2019

AbstractHow and under what circumstances can adjusting the inflation target serve as a stabilization‐policy tool and contribute to welfare improvement? We answer these questions quantitatively with a standard New Keynesian model that includes cost‐push‐type shocks. Our proposed inflation target rule calls for the target to be adjusted in a persistent manner and in the opposite direction to the realization of a cost‐push shock, which is essentially a makeup strategy. The inflation target rule, combined with a Taylor‐type rule, significantly reduces inflation fluctuations originating from cost‐push shocks and mitigates the stabilization trade‐off, resulting in a similar level of welfare to that associated with the Ramsey optimal policy.
Positive impacts of changes to a tertiary hospital after‐hours endocrine and diabetes on‐call service
Authors: Biggin Andrew , Stewart Phoebe , Heels Kristine , Maguire Ann , Ambler Geoffrey R 
Published: December 2019

AimTo examine the impact of changes to the endocrine/diabetes after‐hours service model of care at a major tertiary children's hospital in Australia. The model aimed to enhance the independence of families and reduce dependency on after‐hours calls to health professionals.MethodsThe after‐hours activity was captured prospectively using an iPad with a customised FileMaker database. Data were collected for 9 months prior to and for 8 months after the implementation of a modified model of service. Questionnaires gathered information from endocrine junior medical officers (JMOs) and other hospital staff. Data on emergency department visits were analysed for presentations before and after the implementation of the service changes.ResultsChanges to the after‐hours service resulted in a significant reduction in median calls from 9 (range 0–39) to 2 (range 0–7) per shift. The number of shifts with no calls increased from 2 to 24% and the number of shifts with <3 calls increased from 8 to 60%. Disturbed nights (calls between 10 pm and 6 am) decreased from 75 to 29%. Junior medical officer experience was positive and there was no perceivable increase in workload from in‐hospital staff. The number of endocrine patients presenting to the emergency department did not change significantly following the implementation of the new after‐hours service.ConclusionThis is the only Australian study to prospectively gather accurate on‐call data in order to elucidate the impact of changing a hospital's after‐hours endocrine/diabetes service to a model that enhanced family empowerment and independence. Historical 24‐h on‐call service models are not indispensable, and changes can improve sustainability without compromising patient care.
Hyperarousal captured in increased number of arousal events during pre‐REM periods in individuals with frequent nightmares
Authors: Blaskovich Borbála , Reicher Vivien , Gombos Ferenc , Spoormaker Victor I. , Simor Péter 
Published: December 2019

SummaryThe aim of this study was to investigate hyperarousal in individuals with frequent nightmares (NM participants) by calculating arousal events during nocturnal sleep. We hypothesized an increased number of arousals in NM participants compared with controls, especially during those periods where the probability of spontaneous arousal occurrence is already high, such as non‐rapid eye movement to rapid eye movement transitions (pre‐rapid eye movement periods). Twenty‐two NM participants and 23 control participants spent two consecutive nights in our sleep laboratory, monitored by polysomnography. Arousal number and arousal length were calculated only for the second night, for 10 min before rapid eye movement (pre‐rapid eye movement) and 10 min after rapid eye movement (post‐rapid eye movement) periods, as well as non‐rapid eye movement and rapid eye movement phases separately. Repeated‐measures ANOVA model testing revealed significant Group (NM participants, controls) × Phase (pre‐rapid eye movement, post‐rapid eye movement) interaction in case of the number of arousals. Furthermore, post hoc analysis showed a significantly increased number of arousals during pre‐rapid eye movement periods in NM participants, compared with controls, a difference that disappeared in post‐rapid eye movement periods. We propose that focusing the analyses of arousals specifically on state transitory periods offers a unique perspective into the fragile balance between the sleep‐promoting and arousal systems. This outlook revealed an increased number of arousals in NM participants, reflecting hyperarousal during pre‐rapid eye movement periods.
Transcriptomic signatures of social experience during early development in a highly social cichlid fish
Authors: Nyman Cecilia , Hebert Francois Olivier , Bessert‐Nettelbeck Mathilde , Aubin‐Horth Nadia , Taborsky Barbara 
Published: December 2019

AbstractThe social environment encountered early during development can temporarily or permanently influence life history decisions and behaviour of individuals and correspondingly shape molecular pathways. In the highly social cichlid fish Neolamprologus pulcher, deprivation of brood care permanently affects social behaviour and alters the expression of stress axis genes in juveniles and adults. It is unclear when gene expression patterns change during early life depending on social experience, and which genes are involved. We compared brain gene expression of N. pulcher at two time points during the social experience phase when juveniles were reared either with or without brood care, and one time point shortly afterwards. We compared (a) whole transcriptomes and (b) expression of 79 genes related to stress regulation, in order to define a neurogenomic state of stress for each fish. At developmental day 75, that is, after the social experience phase, 43 genes were down‐regulated in fish having experienced social deprivation, while two genes involved in learning and memory and in post‐translational modifications of proteins (PTM), respectively, were up‐regulated. Down‐regulated genes were mainly associated with immunity, PTM and brain function. In contrast, during the experience phase no genes were differentially expressed when assessing the whole transcriptome. When focusing on the neurogenomic state associated with the stress response, we found that individuals from the two social treatments differed in how their brain gene expression profiles changed over developmental stages. Our results indicate that the early social environment influences the transcriptional activation in fish brains, both during and after an early social experience, possibly affecting plasticity, immune system function and stress axis regulation.
Biopsychosocial assessment and management of persistent orofacial pain
Authors: Penlington C. , Ohrbach R. 
Published: December 2019

AbstractPersistent orofacial pain (OFP) presents as a range of conditions of complex aetiology which include interacting biological, psychological and social aspects. Biopsychosocial factors have an impact on the development, maintenance and severity of pain disorders as well as on their treatment. Since OFP is unlikely to be cured, given current knowledge, patients need to play an active role in using strategies to reduce their pain or the impact it has on their life. This is known as self‐management and provides a firm foundation for tailored medical management strategies. Biopsychosocial considerations have an important role in supporting successful self‐management. This review describes biopsychosocial factors that may be important to consider in routine care settings. Psychological factors that are known to affect outcome include anxiety and depression and, more specifically catastrophising and self‐efficacy. Research into pain management in other conditions also stresses the importance of psychological flexibility, the ability to respond in a helpful way even when this involves maintaining awareness of difficult feelings and thoughts. Patients' understanding of their pain, including beliefs about its meaning and how it should be managed is also important. Social factors include communication and relationship‐building in clinic, both of which are key. Outside the clinic, other social factors might compromise a patients’ ability to engage in treatment, and clinicians need to be alert to their possible presence. Suggestions are made for incorporating biopsychosocial principles into routine assessment and treatment and for deciding when specialist referral to psychology services might be required.
Dietary adaptations and palaeoecology of Lophialetidae (Mammalia, Tapiroidea) from the Eocene of the Erlian Basin, China: combined evidence from mesowear and stable isotope analyses
Authors: Gong Yanxin , Wang Yuanqing , Wang Yang , Mao Fangyuan , Bai Bin , Wang Haibing , Li Qian , Jin Xun , Wang Xu , Meng Jin 
Published: December 2019

AbstractLophialetidae is an extinct group of endemic Asiatic tapiroids that are widely distributed in the Eocene sediments of Asia. Schlosseria magister and Lophialetes expeditus are the most abundant species in this family. However, their dietary and ecological characteristics are largely unknown. For the first time, we reconstruct the palaeodiet and habitat of these two lophialetids using a combination of mesowear and stable carbon isotope analysis of fossil teeth excavated from the Erlian Basin, China. Mesowear analysis (n = 141) suggests that the dietary structure of S. magister and L. expeditus shifted from less to more abrasive diets from ~52 to ~42 Ma. Stable carbon isotope analysis (n = 137) suggests that the habitats of S. magister and L. expeditus became drier and/or more open through time. The dietary shifts of the two lophialetids are consistent with evident changes in habitat. The changes in the diet and habitat were probably related to global climate change during that time period. The gradual drop in global temperatures during the early–middle Eocene led to a drier and more open terrestrial ecosystem in the Erlian Basin, probably resulting in changes in floral composition of the environment inhabited by S. magister and L. expeditus. Hence, herbivores highly susceptible to vegetation modification had to develop new resource exploitation strategies to adapt to these changes. Schlosseria magister, considered to be the sister‐group of L. expeditus and with a low level of ecological flexibility, was unable to adapt to the habitat changes finally becoming extinct at ~45 Ma.
Age‐Related Absorption of the Human Lens in the Near‐Ultraviolet Range
Authors: Pajer Viktor , Rárosi Ferenc , Kolozsvári Lajos , Hopp Béla , Nógrádi Antal 
Published: December 2019

AbstractThe purpose of the present study was to determine the age dependence of the ultraviolet (UV) absorption of the different parts of the human crystalline lens. Cryostat sections of human cadaveric lenses (60 μm) were cut. The UV absorbance of nine samples, derived from different parts of the lens, was determined using a Shimadzu scanning spectrophotometer. The absorbance of the anterior and posterior lens capsules was measured separately. The absorption coefficients were calculated from the measured absorbance and values taken at 280 as well as at 360 nm were compared statistically. ANCOVA analysis of the values taken at 280 and at 360 nm wavelengths shows that correlation between the absorption coefficients and age can be found only in the case of the posterior layers. These results suggest a differential age‐dependent increase of the UV absorption of the posterior layers compared to the anterior ones and can be related to the differential protein expression in the anterior and posterior parts. Posterior crystalline lens capsules have higher absorption coefficients than the anterior ones regardless of age.
Abnormal functions of Leydig cells in crossbred cattle–yak showing infertility
Authors: Sato Yoko , Kuriwaki Ryota , Hagino Shiki , Shimazaki Megumi , Sambuu Rentsenkhand , Hirata Maki , Tanihara Fuminori , Takagi Mitsuhiro , Taniguchi Masayasu , Otoi Takeshige 
Published: December 2019

AbstractIn Mongolia, yak (Bos grunniens) are able to live in alpine areas and their products greatly influence the lives of the local people. Increased vigour in hybridized yak and cattle can offer benefits for livestock farmers. However, male hybrids show reproductive defects resulting from spermatogenesis arrest, affecting the conservation and maintenance of dominant traits in the next generation. The underlying mechanisms involved in hybrid cattle–yak infertility have recently been investigated; however, the genetic cause is still unclear. Androgens and androgen receptor (AR) signalling are required for spermatogenesis. We, therefore, evaluated the expression of AR, 3β‐hydroxysteroid dehydrogenase (3βHSD) and 5α‐reductase 2 (SRD5A2) in Leydig cells to investigate their function in cattle–yak spermatogenesis. Testicular tissues from yaks (1–3 years old) and hybrids (F1–F3, 2 years old) were collected and subjected to immunohistochemistry and image analyses to investigate the expression of each parameter in the Leydig cells. After maturation at 2 years, the expression levels of AR increased and the levels of 3βHSD decreased, but the SRD5A2 levels remained constant in yak. However, the cattle–yak hybrid F2 showed immature testicular development and significantly different expression levels of AR and 3βHSD compared with mature yak. These results suggest that the decreased expression of AR and increased expression of 3βHSD in the Leydig cells of cattle–yak hybrid testes may represent one of the causes of infertility. Our study might help in solving the problem of infertility in crossbreeding.
Prediction of Reynolds Number Effects on Low-Pressure Turbines Using a High-Order ILES Method
Authors: Bolinches-Gisbert M. , Robles David Cadrecha , Corral Roque , Gisbert Fernando 
Published: December 2019

Abstract
A comprehensive comparison between implicit large eddy simulations (ILES) and experimental results of a modern high-lift low-pressure turbine airfoil has been carried out for an array of Reynolds numbers (Re). Experimental data were obtained in a low-speed linear cascade at the Polytechnic University of Madrid using hot-wire anemometry and laser-Doppler velocimetry (LDV). The numerical code is fourth-order accurate, both in time and space. The spatial discretization of the compressible Navier–Stokes equations is based on a high-order flux reconstruction approach while a fourth-order Runge–Kutta method is used to march in time the simulations. The losses, pressure coefficient distributions, and boundary layer and wake velocity profiles have been compared for an array of realistic Reynolds numbers. Moreover, boundary layer and wake velocity fluctuations are compared for the first time with experimental results. It is concluded that the accuracy of the numerical results is comparable to that of the experiments, especially for integral quantities such as the losses or exit angle. Turbulent fluctuations in the suction side boundary layer and the wakes are well predicted too. The elapsed time of the simulations is about 140 h on 40 graphics processor units. The numerical tool is integrated within an industrial design system and reuses pre- and post-processing tools previously developed for another kind of applications. The trend of the losses with the Reynolds number has a sub-critical regime, where the losses scale with Re−1, and a supercritical regime, where the losses scale with Re−1/2. This trend can be seen both in the simulations and in the experiments.