bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection Of Ampicillin Resistance of Escherichia coli Isolated From Chicken Livestock Farming In Surabaya, Indonesia
Authors: Solikhah Tridiganita Intan 
Published: May 2020

The study was carried out to isolate and detect the resistance of E. coli bacteria from four livestock in Surabaya, Indonesia.
This research used 40 samples of chicken thigh meat. The first step is isolated Escherichia coli bacteria using BGBB media (Brillilliant Green Bile Broth), and then used EMBA (Eosin Methylen Blue Agar) media for confirmatory tests, IMViC tests (Indole, Methyl Red, Voges Praskauer, and Citrat) to identify the enterobacteriaceae bacteria, and performed the gram staining test. Determined the Escherichia coli sensitivity to antibiotic by using disk diffusion method.
Resistance test has resulted for ampicillin against E.coli is 100% resistance in all farms. In this research 13 of 40 samples from chicken meat had positive E.coli. The result from electrophoresis product of PCR said that resistance Escherichia coli had a specific band. Total 13 samples of positive Escherichia coli resistance ampicillin has specific DNA band primary shv with 768 bp in length.
Penilaian Kebutuhan Home Pharmacy Care Untuk Pasien Diabetes Mellitus Tipe 2 Yang Disertai Hipertensi Pada Usia Lanjut
Authors: Nurfauzi Yuhansyah , Wahyono Djoko , Rahmawati Fita , Yasin Nanang Munif 
Published: May 2020

Home Pharmacy Care (HPC) merupakan pelayanan yang diharapkan dapat diberikan oleh apoteker untuk penyakit kronis maupun lanjut usia (lansia), namun pelaksanaannya masih jauh dari harapan pihak-pihak yang memerlukannya. Penelitian ini merupakan studi kualitatif pertama di Indonesia yang bertujuan untuk menilai kebutuhan pelayanan HPC untuk pasien lansia yang mengalami Diabetes Mellitus (DM) Tipe 2 disertai dengan hipertensi di Cilacap. Penilaian ini menjadi studi awal yang penting untuk penelitian berikutnya dalam rangka membuat model pelayanan HPC bagi pasien lansia.
Sudut pandang pasien dan tenaga kesehatan digali untuk mengungkapkan kebutuhan HPC. Penelitian ini menggunakan metode kualitatif dan dilakukan dengan survei disertai wawancara mendalam. Survei dilakukan dengan cara menyebarkan kuesioner kepada apoteker untuk mendeskripsikan aktivitas yang perlu dilakukan dalam HPC beserta kendalanya dan merinci keterampilan yang diperlukan untuk melaksanakannya. Subjek penelitian berjumlah 32 orang apoteker, sedangkan informan terdiri dari 4 dokter, 3 perawat dan 5 pasien sehingga memenuhi kejenuhan data sebagai kecukupan sampel kualitatif.
Hanya 3 apoteker (9,4%) dari keseluruhan subjek penelitian yang pernah memberikan pelayanan HPC, sedangkan sisanya belum pernah melaksanakannya, namun, semua pihak baik apoteker, petugas kesehatan maupun pasien menyatakan perlunya HPC untuk pasien dengan kondisi tersebut. Kendala yang terungkap dalam penelitian ini adalah keterbatasan sumber daya manusia, waktu dan finansial. Apoteker membutuhkan keterampilan edukasi obat, diet, aktivitas fisik dan pemeriksaan kaki bagi pasien disertai dengan kemampuan komunikasi yang memadai. Home pharmacy care masih jarang dipraktikkan di Cilacap, tetapi perlu untuk dilaksanakan dengan selektif karena didukung oleh dokter, perawat serta pasien. Kebutuhan HPC ini relevan untuk ditindaklanjuti dengan pembentukan model pelayanan kefarmasian.
Aktivitas Antioksidan dan Potensi Tabir Surya Serum Ekstrak Terpurifikasi Daun Wangon (Olax psittacorum (Willd.) Vahl.)
Authors: Harjanti Reslely , Nilawati Anita 
Published: May 2020

Perkembangan sediaan kosmetika herbal saat ini semakin pesat. Salah satunya adalah serum yang disukai masyarakat karena lebih nyaman digunakan dan lebih mudah menyebar di permukaan kulit. Pada umumnya serum mengandung senyawa antioksidan yang poten sebagai anti aging di mana formulanya dikaitkan dengan komponen antioksidan di dalamnya. Ekstrak etanol daun wangon telah diteliti mengandung senyawa golongan flavonoid yang mempunyai potensi antioksidan. Penelitian ini bertujuan untuk membuat ekstrak terpurifikasi daun wangon selanjutnya dibuat sediaan kosmetika serum yang diuji mutu fisik dan stabilitas antioksidannya serta dilakukan pengujian terkait potensinya sebagai tabir surya sehingga diharapkan dapat dilakukan inovasi terhadap sediaan serum yang dihasilkan.
Penelitian diawali dengan pembuatan ekstrak dengan pelarut etanol 96%. Selanjutnya dilakukan fraksinasi terhadap ekstrak kasar dengan metode ekstraksi cair-cair yang berturut-turut menggunakan pelarut n-heksana dan etil asetat. Kandungan flavonoid dalam ekstrak terpurifikasi dimonitoring secara KLT. Selanjutnya dibuat formulasi sediaan serum dengan 1,1 % zat aktif ekstrak terpurifikasi daun wangon dan dilakukan uji mutu fisik terhadap sediaan tersebut meliputi organoleptis, pH, viskositas dan homogenitasnya. Selanjutnya ekstrak terpurifikasi dan sediaan serum diuji aktivitas antioksidannya dengan metode DPPH dan penentuan aktivitas sebagai tabir surya dengan parameter nilai SPF.
Hasil penelitian menunjukkan bahwa ekstrak terpurifikasi daun wangon mempunyai nilai IC50 41,98 µg/mL lebih baik jika dibandingkan dengan ekstrak kasar dengan IC50 65,32 µg/mL. Sediaan serum mempunyai nilai IC50 71,30 µg/mL dan tergolong antioksidan kuat. Setelah sediaan disimpan selama 30 hari potensi antioksidannya tetap stabil. Selain itu serum tersebut juga mempunyai potensi sebagai tabir surya dengan nilai SPF 19,50±0,02 yang kategorikan memberikan perlindungan menengah terhadap sinar UV B.
Efektivitas dan Keamanan Terlipressin pada Acute Variceal Haemorhage: Sebuah Kajian Naratif
Authors: Halim Steven Victoria , Setiawan Eko 
Published: May 2020

Penanganan secara cepat dan tepat untuk menghentikan pendarahan merupakan salah satu pilar penting dalam tata laksana kasus acute variceal haemorhage (AVH). Terdapat beberapa pilihan obat yang dapat digunakan untuk menghentikan pendarahan yang terjadi, salah satunya dengan menggunakan Terlipressin, sebuah obat yang baru masuk Indonesia pada tahun 2019. Pengetahuan terkait efektivitas dan keamanan penggunaan Terlipressin sangat diperlukan untuk menunjang penggunaan obat yang optimal dan mencegah penggunaan obat secara berlebihan khususnya pada periode awal keberadaan obat tersebut di Indonesia.
Kajian pustaka ini bersifat naratif dan memaparkan beberapa data terkait: profil dasar Terlipressin, efektivitas dan keamanan penggunaan pada kasus AVH yang berbasis bukti (evidence-based), posisi terapi menggunakan Terlipressin pada beberapa pedoman terapi, serta komparasi biaya terapi Terlipressin bila dibandingkan obat vasoaktif lainnya. Hanya pustaka terpublikasi dalam bentuk kajian sistematis dan meta-analisis yang digunakan dalam tinjauan efektivitas dan keamanan.
Terlipressin memiliki beberapa karakteristik farmakokinetik dan farmakodinamik yang dapat memberikan keuntungan terkait teknis pemberian dibandingkan dengan beberapa obat vasoaktif yang lain. Namun demikian, hal tersebut tidak berarti bahwa Terlipressin lebih unggul dibandingkan dengan jenis vasoaktif yang lain. Hasil kajian terhadap tiga (3) artikel kajian sistematis dan meta-analisis menunjukkan bahwa efektivitas dan keamanan Terlipressin tidak lebih baik secara signifikan dibandingkan dengan obat vasoaktif lain. Oleh karena itu, Terlipressin bukan merupakan satu-satunya obat yang direkomendasikan sebagai pilihan pertama vasoaktif pada tata-laksana AVH. Sebagai obat yang relatif baru, biaya penggunaan Terlipressin lebih tinggi dibandingkan vasoaktif pilihan pertama yang lain. Dengan mempertimbangkan beberapa hal tersebut, Terlipressin sebaiknya digunakan pada pasien yang dapat memperoleh keuntungan klinis optimal, misalnya pasien dengan kontraindikasi atau riwayat efek samping dengan vasoaktif yang lain.
The burden of acute pancreatitis and early experience of step-up approach for acute necrotizing pancreatitis at Military Hospital
Authors: Rawal Sushil Bahadur , Laudari Uttam , Khatiwada Basanta , Thapa Anup , Jha Manoj Kumar , Rayamajhi Jyoti , Malla Srijan 
Published: May 2020

Introduction: Twenty-five percent of acute pancreatitis develops severe acute pancreatitis (SAP). SAP patients have prolonged hospitalization and require a substantial amount of manpower and hospital resources. The aim of this study was to give an insight into the burden of acute pancreatitis at a tertiary level military hospital and to share the experience of managing pancreatic necrosis with the application of the step-up approach.
Methods: A prospective descriptive study of consecutive patients was conducted at Shree Birendra Army Hospital from 2017 June to 2017 November. Patients with a diagnosis of acute pancreatitis admitted were stratified according to severity as per Revised Atlanta classification 2012. Patients with acute necrotizing pancreatitis were enrolled for the step-up approach. Patient’s demography, baseline investigations, radiology, prognostic scoring scales and type of intervention were analyzed. The burden of severe acute pancreatitis at our center and the early experience of the step-up approach at our centre within the study period were studied.
Results: Out of 35 five patients with acute pancreatitis, 7 (20%) subsequently developed severe acute pancreatitis. Among them, five patients subsequently developed necrotizing pancreatitis who were managed according to the step-up approach. Out of them, three patients subsequently underwent operative intervention in the form of minimally invasive procedures like percutaneous drain placement, laparoscopic necrosectomy, video-assisted retroperitoneal debridement, and open necrosectomy.
Conclusion: Acute pancreatitis is a common presentation at our centre. The step-up approach in the management of acute necrotizing pancreatitis is a safe and feasible approach.
Clinico-pathological evaluation of cervical lymphadenopathy
Authors: Mukhia Rupesh , Bhatta Sushama , Simkhada Ganesh , Thapa Abishek , Mukhia Rupashia 
Published: May 2020

Introduction: Cervical lymph nodes are peripheral lymphoid organs distributed in the neck. The term lymphadenopathy refers to nodes that are palpable and abnormal in size, consistency or numbers. Diagnosis of cervical lymphadenopathy can vary from neoplasm to various kinds of infection. Accurate diagnosis of the cause of lymphadenopathy can sometimes be challenging and can lead to delay in diagnosis causing delayed treatment and subsequent health issues.
Methods: A prospective observational study was carried out on 78 patients of cervical lymphadenopathy who presented in the surgical outpatient department of KIST Medical College. Detailed history, physical examination and necessary investigations including fine needle aspiration cytology were done in all patients
Results: Most patients were between 21 to 40 years of age. The commonest cause for cervical lymphadenopathy was reactive lymphadenitis (53.84%) followed by Tuberculosis (34.61%). Secondary metastasis was seen in 7 patients (8.97%). In tubercular lymphadenitis, the anterior triangle group was the most commonly involved group of cervical lymph nodes (74 %), followed by the posterior triangle and supraclavicular equally (11.11%).
Conclusions: Reactive Lymphadenopathy is the commonest cause of cervical lymphadenopathy. However, variable diagnosis can be possible from non-neoplastic to neoplastic condition.
Early experience with ultrasound guided pneumatic reduction of intussusception using locally assembled equipment in Nepal
Authors: Adhikari Suman Bikram 
Published: May 2020

Introduction: Intussusception is one of the commonest emergency conditions in children. Pneumatic reduction of intussusception, a minimally invasive technique, has a higher success rate and lower incidence of complications as compared to barium enema & hydrostatic reduction and also omits the need for unnecessary laparotomy. The aim of this study was to evaluate the results of the pneumatic reduction in our hospital as a treatment of idiopathic pediatric ileocolic intussusception and to identify the pretreatment factors associated with pneumatic reduction failure.
Methods: This was a prospective analytical study. A total of 12 children were enrolled in the study between January to November 2018 at Nepal National Hospital, Kathmandu. Patients were given air enema under Ultrasound-guidance, using locally assembled equipment. All procedures were performed under intravenous anesthesia. The intraluminal pressure was monitored with a pressure gauge and was not permitted to go above 100 mmHg. A total of three attempts of 3 minutes each were allowed.
Results: Average age of the patients was 2.7 years, with a male-female ratio of 3:1. Eleven (92%) of the cases were successfully reduced while 1 (8%) case failed to reduce. No bowel perforation occurred in this study. The mean duration of symptoms before presentation was 42 hours. The mean length of intussusceptum was 3.058 cm. the mean duration of pneumatic reduction was 1.97 minutes and total intervention time i.e. from induction of anesthesia to reversal from anesthesia was 18.55 minutes.
Conclusion: The technique described is easy to assemble, safe and effective. I recommend it for regular use in pneumatic reduction of intussusception, especially in centers with limited resources.
Diagnostic dilemma in a large choledochal cyst
Authors: Gurung Tul Maya , Koirala Rabin , Shrestha Amit 
Published: May 2020

Choledochal cysts are rare congenital anomalies that have a variable presentation with occasional diagnostic and therapeutic dilemmas.
Our case is a 14-year female presented with recurrent abdomen pain with confusing diagnostic findings, suggestive of a large choledochal cyst (22x29x17cm) initially managed by percutaneous tube biliary drainage and later underwent excision and HPE revealing non-malignant findings.
The choledochal cyst should always be a differential diagnosis for any patient presenting with an abdominal mass and should be managed accordingly.
A rare encounter of obstructed direct inguinal hernia
Authors: Singh Amit Kumar , Rajbhandari Nripesh , Malla Balaram , Bhatta Gakul 
Published: May 2020

The direct inguinal hernia has a wider neck and thus usually doesn’t present as strangulation or incarceration in comparison to the indirect component. When direct inguinal hernias are untreated for a longer duration, they may get strangulated and incarcerated. Hence such long-standing direct hernias with features of intestinal obstruction and /or peritonism should be promptly seen and diagnosed to prevent massive and unwanted intestinal resection. We are reporting a case of 83-year-old male presented to Surgical Emergency Department of Dhulikhel Hospital, Kathmandu University hospital with complaints of swelling in the right inguinoscrotal region for 12 years and progressed to become irreducible and painful for 12 hours. Clinically he had an acute intestinal obstruction. Intra-operatively we found a direct hernia containing congested small bowel loops and toxic fluids. The toxic fluid was suctioned and after confirming viability, modified Bassini’s repair was done with reinforcement of the posterior wall.
Even direct inguinal hernia of longer duration can cause acute or sub-acute intestinal obstruction with or without features of peritonism. This complication is more common in elderly patients.
‘मैले नजन्माएको छोरो’ कथाको समाख्यान विश्लेषण "Maile Najanmayeko Chhoro" Kathako Samakhyan Vishleshan
Authors: लुइटेल Luitel गोविन्दप्रसाद  Govindaprasad 
Published: May 2020

प्रस्तुत अध्ययन ‘मैले नजन्माएको छोरो’ कथाको समाख्यान विश्लेषणमा आधारित रहेको छ । समाख्यान विश्लेषण समाख्यानको संरचना विश्लेषण गर्ने समाख्यानशास्त्रीय अवधारणा हो । यसमा समाख्यानअन्तर्गत विवेच्य कथाका समाख्याता, समाख्यानात्मक वाच्यत्व र कथनीयताको निरूपण गरिएको छ । यस अध्ययनमा प्रस्तुत कथामा कथासम्बद्धताका आधारमा बहिर्निष्ठ, पात्रसम्बद्धताका आधारमा समाख्याताले कथा संसारभित्र पात्रका रूपमा उपस्थित भएर आफ्नो कथा आफै भनेकाले संलग्न स्वकथनात्मक समाख्याता र समाख्याताको खुलाइका आधारमा आफूलाई सन्दर्भ बनाएर खुला रूपमा प्रस्तुति गरेकाले यस कथाको समाख्याता खुला समाख्याता हो भन्ने निष्कर्ष प्राप्त भएको छ । यस कथामा समाख्याताले आफूलाई सन्दर्भ बनाएकाले समाख्याताबारे धेरै सूचनाहरू प्राप्त गर्न सकिन्छ र कथामा समाख्याताको आवाज पनि उच्च छ । प्रस्तुत कथामा समाख्याता आफैलाई सन्दर्भ बनाएर जीवनजगत्प्रतिको आत्मगत अभिव्यक्ति, निराशा र अस्मिताबोधलाई कथनीयताका रूपमा प्रस्तुत गरिएको पाइन्छ भन्ने निष्कर्ष अध्ययनबाट प्राप्त भएको छ ।
ANALISA KARAKTER TOKOH ANDREA DALAM FILM THE DEVIL WEARS PRADA BERDASARKAN PENDEKATAN HUMANISTIK
Authors: Mulyati Sri - 
Published: May 2020

Karya sastra adalah media yang digunakan oleh pengarang untuk menyampaikan ide, pemikiran, dan pengalaman pengarang yang disampaikan kepada pembaca. Novel merupakan karya sastra yang menceritakan kehidupan seorang atau beberapa orang tokoh. Kehidupan tokoh tersebut diceritakan dengan gaya pengarang yang bertujuan untuk menghibur dan memberikan pelajaran bagi para pembacanya. Dalam membuat novel pengarang menceritakan pengalaman hidup yang mungkin terjadi dalam hidup manusia di dunia nyata. Film dianggap sebagai media komunikasi yang ampuh terhadap masyarakat yang menjadi sasarannya, karena sifatnya yang audio visual, yaitu gambar dan suara yang hidup. Film mampu bercerita banyak dalam waktu singkat. Ketika menonton film, penonton seakan-akan dapat menembus ruang dan waktu. Film bahkan dapat mempengaruhi penonton.Objek penelitian ini adalah film, yaitu film The Devil Wears Prada. Dalam film ini penulis fokus pada karakter tokoh Andrea, asisten pribadi pimpinan redaksi majalah fashion, Runway, Miranda Priestly. “The Devil Wears Prada” adalah film drama komedi Amerika tahun 2006 yang dibuat berdasarkan novel Lauren Weisberger tahun 2003 dengan nama yang sama. Film ini menceritakan tentang sosok wanita pemimpin editor sebuah perusahaan majalah fashion mode terkemuka di Paris, Runway, dan bagaimana hubungannya dengan salah satu asisten pribadinya, AndreaAndrea seorang wanita biasa yang sederhana, tidak berpenampilan menarik, namun rendah hati, supel, dan cerdas, yang diberi kesempatan oleh Miranda untuk bekerja sebagai sekertaris pribadinya yang kedua setelah Emily. Sosok yang pada akhirnya mampu menyesuaikan diri dengan budaya kerja di kantor ia bekerja yaitu kantor majalah fashion yang terkemuka, Runway, dan memahami tugas-tugas kerjanya serta dapat menyelesaikan semuanya dengan baik. Keywords: film, fashion, women carrier
Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components
Authors: Nazarova M.S. , Stanislavchuk M.A. , Burdeina L.V. 
Published: May 2020

Annotation. There is a tendency for an increase of incidence of coronary heart disease (CHD) among young people, with the development of acute myocardial infarction (MI) in the absence of marked atherosclerosis and obstruction of the coronary arteries. Antiphospholipid syndrome (APS), cellular and humoral mediators of immunoinflammatory activation of endothelium are considered as factors of non-obstructive damage to the coronary vessels. New trigger factors for endothelial dysfunction, cardiac fibrosis, and myocardial hypertrophy include toll-like receptors (TLR2 and TLR4). Antiphospholipid antibodies may act as endogenous activators of toll-like receptors. The study of the association between APS components and expression of toll-like receptors in patients with CHD with postinfarction cardiosclerosis remains relevant. The aim is to study the levels of TLR2 and TLR4 and its relationship with APS components in men with postinfarction cardiosclerosis. 164 patients with stable CHD with postinfarction cardiosclerosis (100% of men, age 53.0±9.14 years) and 48 men of control group (without CHD) were examined. Levels of total antiphospholipid antibodies (aPL), antibodies to β2-glycoprotein 1 (anti-β2-GP 1) IgG and IgM, TLR2, TLR4 in serum were determined by ELISA. The studies were conducted in compliance with bioethical standards. Statistical processing of the results was performed in SPSS Statistics 22.0. Determined that men with postinfarction cardiosclerosis showed higher levels of TLR2 and TLR4 (in 1.8–2.2 times, p<0.001) in serum than control group. Higher (in 1.2–1.4 times) levels of TLR2 and TLR4 were found in patients who underwent Q-MI, recurrent MI and had clinical manifestations of APS (stroke, transient ischemic attacks, livedo reticularis). An increase TLR2 and TLR4 levels was closely associated with an increase of aPL and anti-β2-GP 1 of IgG (r = | 0.31 - 0.51 |, p<0.01). Thus, in men with postinfarction cardiosclerosis who had aPL and anti-β2-GP 1 IgG positivity had an unfavorable immunoinflammatory pattern, that characterized by increased expression of TLR2 and TLR4. The imbalance in TLR2 and TLR4 is more pronounced in young CHD patients and associated with Q-MI and recurrent MI.
Daily interleukin-1β variability in women with rheumatoid arthritis associated with fibromyalgia
Authors: Perebetyuk L.S. , Moroz L.V. , Shevchuk S.V. , Segeda Y.S. , Shtatko O.E. 
Published: May 2020

Annotation. Disorders of circadian rhythms are considered to be one of the factors associated with the development of joint diseases. In patients with rheumatoid arthritis (RA) disorders of the neurohumoral regulation of circadian rhythms is a factor in the increased production of inflammatory cytokines in the joint tissues. In 30 % of RA patients, concomitant fibromyalgia (FM) is found, in which disorders of circadian regulation can deepen. Circadian features of the production of pain mediators and inflammation in RA patients with comorbid fibromyalgia (FM) have not been established. The aim of this study was to investigate the daily variability of serum interleukin-1β levels in RA patients depending on the comorbidity of FM. Materials and methods: 49 patients with rheumatoid arthritis (RA) (100 % female) aged 46.8 (39–53) years, with moderate and high disease activity (DAS28>3.2), of which 21 (42.9 %) patients with concomitant fibromyalgia (FM). Diagnosis of RA was established by ACR/EULAR criteria (2010), FM was diagnosed by mACR 2010. Blood sampling was performed twice daily (at 08-00 and at 20-00), the level of IL-1β in the blood was determined by enzyme immunoassay. The studies were conducted according to bioethical standards. Statistical processing of the results was performed by SPSS Statistics 22.0. Results: In RA patients circadian fluctuations of IL-1β level in blood were registered. IL-1β levels (08-00) in the morning was higher (1.2–1.3 times) than in the evening (20-00). In the presence of comorbid FM, patients with RA had significant increase in evening and average daily levels of IL-1β (1.3–1.6 times) than in RA patients without FM. Daily fluctuations of IL-1β correlate with RA activity and the fibromyalgia scale. Conclusions: In RA patients disorders of the circadian production of proinflammatory IL-1β, which are exacerbated under FM conditions, were detected. Increasing evening production of IL-1β correlated with the prevalence of pain index in RA patients with FM. Thus, disorders of IL-1β circadian production in RA patients can be integrated into the mechanisms of central sensitization and FM development.