bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of intravitreal sodium iodate injection on retinal degeneration following vitrectomy in rabbits
Authors: Ahn So Min , Ahn Jungryul , Cha Seongkwang , Yun Cheolmin , Park Tae Kwann , Kim Young-Jin , Goo Yong Sook , Kim Seong-Woo 
Published: October 2019

AbstractWe sought to develop and characterize outer retinal degeneration induced by intravitreal injection of sodium iodate (SI) after vitrectomy in rabbits. To determine the effective dose of SI, the right eyes of 19 male New Zealand white rabbits received an intravitreal injection of SI or sham. Based on the dose-dependence results, 0.4 mg of SI in 0.05 mL of total volume was injected into the right eyes of 10 rabbits at two weeks after vitrectomy. In the dose-dependence study, localized retinal atrophy was observed with 0.3- and 0.4-mg SI injections without vitrectomy. Severe and diffuse retinal atrophy was identified by spectral-domain optical coherence tomography (SD-OCT) at one month after a 0.5-mg SI injection following vitrectomy. In the second experiment, 0.4 mg of SI in 0.05 mL was injected, and the severity of outer retinal degeneration was graded as one of two types according to electroretinography (ERG) response change. There was no response on ERG in complete retinal degeneration, 30% of all 10 rabbits. Intravitreal injection of 0.4 mg of SI into vitrectomized rabbit eyes induces diffuse outer retinal degeneration, and the degree of retinal degeneration can be evaluated through in vivo ophthalmic examination.
Cigarette smoke affects the onco-suppressor DAB2IP expression in bronchial epithelial cells of COPD patients
Authors: Anzalone Giulia , Arcoleo Giuseppe , Bucchieri Fabio , Montalbano Angela M. , Marchese Roberto , Albano Giusy D. , Di Sano Caterina , Moscato Monica , Gagliardo Rosalia , Ricciardolo Fabio L. M. , Profita Mirella 
Published: October 2019

AbstractCigarette smoke is a risk factor for COPD and lung cancer. In cancer, epigenetic modifications affect the expression of Enhancer of Zester Homolog 2 (EZH2), and silenced disabled homolog 2 interacting protein gene (DAB2IP) (onco-suppressor gene) by Histone H3 tri-methylation in lysine 27 (H3K27me3). In“ex vivo”studies, we assessed EZH2, H3K27me3 and DAB2IP immunoreactivity in bronchial epithelial cells from COPD patients (smokers, ex-smokers), Smoker and control subjects. In“in vitro” experiments we studied the effect of cigarette smoke extract (CSE) on EZH2/H3K27me3/DAB2IP expression, apoptosis, invasiveness, and vimentin expression in 16HBE, primary cells, and lung cancer cell lines (A549) long-term exposed to CSE. Finally, in “in vitro”studies, we tested the effect of GSK343 (selective inhibitor of EZH2). EZH2 and H3K27me3 expression was higher, while DAB2IP was lower levels, in bronchial epithelium from COPD and Smokers than in Controls. CSE increased EZH2, H3K27me3 expression and decreased DAB2IP, cell apoptosis and invasiveness in epithelial cells. GSK343 restored the effects of CSE. Cigarette smoke affects EZH2 expression, and reduced DAB2IP via H3K27me3 in COPD patients. The molecular mechanisms associated with EZH2 expression, generate a dysregulation of cell apoptosis, mesenchymal transition, and cell invasiveness in bronchial epithelial cells, encouraging the progression of airway inflammation toward lung cancer in COPD patients.
A novel phospho-modulatory mechanism contributes to the calcium-dependent regulation of T-type Ca2+ channels
Authors: Chemin Jean , Stamenic Tamara Timic , Cazade Magalie , Llinares Jodie , Blesneac Iulia , Todorovic Slobodan M. , Lory Philippe 
Published: October 2019

AbstractCav3 / T-type Ca2+ channels are dynamically regulated by intracellular Ca2+ ions, which inhibit Cav3 availability. Here, we demonstrate that this inhibition becomes irreversible in the presence of non-hydrolysable ATP analogs, resulting in a strong hyperpolarizing shift in the steady-state inactivation of the residual Cav3 current. Importantly, the effect of these ATP analogs was prevented in the presence of intracellular BAPTA. Additional findings obtained using intracellular dialysis of inorganic phosphate and alkaline phosphatase or NaN3 treatment further support the involvement of a phosphorylation mechanism. Contrasting with Cav1 and Cav2 Ca2+ channels, the Ca2+-dependent modulation of Cav3 channels appears to be independent of calmodulin, calcineurin and endocytic pathways. Similar findings were obtained for the native T-type Ca2+ current recorded in rat thalamic neurons of the central medial nucleus. Overall, our data reveal a new Ca2+ sensitive phosphorylation-dependent mechanism regulating Cav3 channels, with potentially important physiological implications for the multiple cell functions controlled by T-type Ca2+ channels.
Hurricane María’s Precipitation Signature in Puerto Rico: A Conceivable Presage of Rains to Come
Authors: Ramos-Scharrón Carlos E. , Arima Eugenio 
Published: October 2019

AbstractThe effects of global climate change on the intensity of tropical cyclones are yet to be fully understood due to the variety of factors that affect storm intensity, the limited time spans of existing records, and the diversity of metrics by which intensity is characterized. The 2017 North Atlantic hurricane season induced record-breaking economic losses and caused hundreds of fatalities, and for many represents a presage of what the future holds under warmer tropical sea surface temperatures. This article focuses on one such major hurricane, María, and answers the question of how this event compares to the historical record of tropical storms that have assailed the island of Puerto Rico since 1898. Comparisons relied on interpolated weather station total rainfall and maximum 24-h rainfall intensities. María proved to have the greatest 24-h rain intensities among all storms recorded in Puerto Rico, yielding maximum 24-h recurrence intervals greater than 250 years for about 8% of the island.
Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells
Authors: Wörsdörfer Philipp , Dalda Nahide , Kern Anna , Krüger Sarah , Wagner Nicole , Kwok Chee Keong , Henke Erik , Ergün Süleyman 
Published: October 2019

AbstractOrganoids derived from human pluripotent stem cells are interesting models to study mechanisms of morphogenesis and promising platforms for disease modeling and drug screening. However, they mostly remain incomplete as they lack stroma, tissue resident immune cells and in particular vasculature, which create important niches during development and disease. We propose, that the directed incorporation of mesodermal progenitor cells (MPCs) into organoids will overcome the aforementioned limitations. In order to demonstrate the feasibility of the method, we generated complex human tumor as well as neural organoids. We show that the formed blood vessels display a hierarchic organization and mural cells are assembled into the vessel wall. Moreover, we demonstrate a typical blood vessel ultrastructure including endothelial cell-cell junctions, a basement membrane as well as luminal caveolae and microvesicles. We observe a high plasticity in the endothelial network, which expands, while the organoids grow and is responsive to anti-angiogenic compounds and pro-angiogenic conditions such as hypoxia. We show that vessels within tumor organoids connect to host vessels following transplantation. Remarkably, MPCs also deliver Iba1+ cells that infiltrate the neural tissue in a microglia-like manner.
Theoretical Insights on the High Pressure Behavior of Pentazolate Anion Complex [Co(H2O)4(N5)2]·4H2O
Authors: Zhao Guozheng , Li Huili , Jia Jianfeng , Wu Haishun , Lu Ming 
Published: October 2019

AbstractPeriodic dispersion corrected density functional theory (DFT) calculations were carried out to examine the Hirshfeld surface, two dimensional (2D) fingerprint plots, crystal structure, molecular structure and density of state of all-nitrogen pentazolate anion complex [Co(H2O)4(N5)2]·4H2O under hydrostatic pressure from 0 to 20 GPa. The GGA/PW91-OBS method was applied in the present study. The intercontacts in [Co(H2O)4(N5)2]·4H2O were analyzed by Hirshfeld surfaces and 2D fingerprint plots. With ascending pressure, the lattice constants, compression rates, bond lengths, bond angles, and density of states change irregularly. Under 11.5, 13.0 and 15.8 GPa, covalent interaction competition is obvious between Co−N and Co−O bonds. It is possible to achieve orderly modification and regulation of the internal structure of [Co(H2O)4(N5)2]·4H2O by applied pressure. This is in accordance with the results from density of states analysis. The external compression causes the nonuniformity of electron density and the differential covalent interaction between pentazolate anion, coordinated water and atom Co. It is of great significance to interpret inter/intramolecular interaction and structural stability of [Co(H2O)4(N5)2]·4H2O and provide theoretical guidance for the design of metal complexes of all-nitrogen pentazolate anion.
Berberine decreases plasma triglyceride levels and upregulates hepatic TRIB1 in LDLR wild type mice and in LDLR deficient mice
Authors: Singh Amar Bahadur , Liu Jingwen 
Published: October 2019

AbstractTRIB1 is a GWAS locus associated with plasma cholesterol and triglycerides (TG) levels. In mice, liver-specific overexpression of TRIB1 lowers plasma lipid levels. Berberine (BBR) is a natural lipid lowering drug that reduces plasma LDL-cholesterol (LDL-C), total cholesterol (TC) and TG in hyperlipidemic patients and in mice by mechanisms involving upregulation of hepatic LDL receptor (LDLR). Here, we demonstrated that BBR treatment reduced plasma LDL-C, TC and TG in LDLR wildtype (WT) mice fed a high fat and high cholesterol diet and it only lowered TG in LDLR WT mice fed a normal chow diet. In hypercholesterolemic LDLR deficient mice (Ldlr−/−), BBR treatment reduced plasma TG levels by 51% compared to the vehicle control without affecting plasma cholesterol levels. Hepatic gene expression analysis revealed that Trib1 mRNA levels were significantly elevated by BBR treatment in all three mouse models and increases of Trib1 mRNA expression were associated with reduced expression of lipogenic genes including Cebpa, Acc1 and Scd1. In vitro studies further demonstrate that BBR induces TRIB1 mRNA expression by a transcriptional mechanism via ERK signaling pathway. These new findings warrant future in vivo studies to determine the causal role of Trib1 in BBR-mediated TG lowering independent of LDLR regulation.
Gene expression profile of Aedes aegypti females in courtship and mating
Authors: Alonso Diego Peres , Campos Melina , Troca Heitor , Kunii Rafael , Tripet Frédéric , Ribolla Paulo Eduardo Martins 
Published: October 2019

AbstractAedes aegypti is the most synanthropic and anthropophilic mosquito of Culicidae. This species always cohabits with humans and is extremely opportunistic. Vector dispersal is directly related to the ability of the females on successfully finding a mate in a generally patchy urban scenario. In the present work, we investigate transcriptional changes in Ae. aegypti females during the courtship process and after mating. We observe a substantial alteration in gene expression triggered just upon contact with Ae. aegypti males, which in turn was not fully correlated to the changes triggered by the contact. After analysing shared significant differentially regulated genes between conspecific contact and insemination, the major part of the observed transcriptomic change triggered by contact is reversed after mating, indicating an intermediary situation between naive and mating conditions that we hypothesize to be crucial for mating success. Upon contact, several chemosensory related genes are repressed, especially odorant binding proteins. Most of these genes return to higher expression rates after mating. None of these genes are significantly regulated by the encounter of a different species, Aedes albopictus. The results presented here might be applied to an innovative control approach focusing on the semiochemical systems of mosquitoes in an effort to disrupt undesirable host–insect interaction to reduce the risk of pathogen transmission to humans.
11C-methionine-PET for differentiating recurrent brain tumor from radiation necrosis: radiomics approach with random forest classifier
Authors: Hotta Masatoshi , Minamimoto Ryogo , Miwa Kenta 
Published: October 2019

AbstractDifferentiating recurrent brain tumor from radiation necrosis is often difficult. This study aims to investigate the efficacy of 11C-methionine (MET)-PET radiomics for distinguishing recurrent brain tumor from radiation necrosis, as compared with conventional tumor-to-normal cortex (T/N) ratio evaluation. We enrolled 41 patients with metastatic brain tumor or glioma treated using radiation therapy who underwent MET-PET. The area with a standardized uptake value > 1.3 times that of the normal brain cortex was contoured. Forty-two PET features were extracted and used in a random forest classifier and the diagnostic performance was evaluated using a 10-fold cross-validation scheme. Gini index was measured to identify relevant PET parameters for classification. The reference standard was surgical histopathological analysis or more than 6 months of follow-up with MRI. Forty-four lesions were used for the analysis. Thirty-three and 11 lesions were confirmed as recurrent brain tumor and radiation necrosis, respectively. Radiomics and T/N ratio evaluation showed sensitivities of 90.1% and 60.6%, and specificities of 93.9% and 72.7% with areas under the curve of 0.98 and 0.73, respectively. Gray level co-occurrence matrix dissimilarity was the most pertinent feature for diagnosis. MET-PET radiomics yielded excellent outcome for differentiating recurrent brain tumor from radiation necrosis, which outperformed T/N ratio evaluation.
Prevalence and correlates of suicide attempt among Chinese individuals receiving methadone maintenance treatment for heroin dependence
Authors: Zhong Bao-Liang , Xie Wu-Xiang , Zhu Jun-Hong , Lu Jin , Chen Hongxian 
Published: October 2019

AbstractTo date, there have been very limited studies regarding the clinical epidemiology of attempted suicide in Chinese individuals with heroin-dependence. The objective of this study was to examine the prevalence and correlates of suicide attempt in Chinese individuals receiving methadone maintenance treatment for heroin dependence. Demographic, clinical, and psychosocial data of 603 methadone-maintained patients with heroin dependence were collected with a standardized self-administered questionnaire. The presence of suicide attempt and antisocial personality disorder was assessed by using a single question and the Mini-International Neuropsychiatric Interview 5.0. The one-month and lifetime prevalence rates of suicide attempt were 9.5% and 34.2%, respectively. In multivariable logistic regression, lifetime suicide attempt was significantly associated with female gender (OR = 2.81), being 20–39 years old (OR = 2.73), an education level of primary school or lower (OR = 2.07), poor economic status (OR = 3.06), injecting heroin before methadone maintenance treatment (OR = 2.92), depressive symptoms (OR = 3.46), anxiety symptoms (OR = 1.88), and antisocial personality disorder (OR = 2.85). Suicide attempt is very prevalent among Chinese individuals receiving methadone maintenance treatment for heroin dependence. Services for patients with heroin dependence in methadone maintenance treatment clinics in China should include psychosocial supports, periodic screening for suicide attempt and other suicidal behaviors and, when needed, psychiatric treatment and crisis intervention.
Compact Ku‐band LTCC bandpass filter using folded dual‐composite right‐ and left‐handed resonators
Authors: Shen Guangxu , Che Wenquan 
Published: October 2019

A compact Ku‐band bandpass filter is proposed by using low‐temperature cofired ceramic (LTCC) technology in this Letter. For miniaturisation, the folded dual‐composite right‐ and left‐handed (D‐CRLH) resonator is introduced. By folding the inter‐digital capacitor and microstrip section inductor in multiple metal layers, the circuit size of D‐CRLH resonator can be reduced to one third of the planner one. Furthermore, the additional transmission loss caused by reverse currents is also investigated. To this end, a very compact LTCC filter is designed, fabricated, and measured, with the core circuit size smaller than 0.91 × 0.95 × 0.29 mm3. The minimum insertion loss is 1.95 dB at 13 GHz while with wide stopband suppression from 14.3 to 33.1 GHz.
Experimental demonstration of waveform‐selective metasurface varying wireless communication characteristics at the same frequency band of 2.4 GHz
Authors: Ushikoshi D. , Tanikawa M. , Asano K. , Sanji K. , Ikeda M. , Anzai D. , Wakatsuchi H. 
Published: October 2019

The authors report a waveform‐selective metasurface that operates at 2.4 GHz band, i.e. one of ISM (Industrial, Scientific and Medical) bands. This structure gives us an additional degree of freedom to control electromagnetic waves and absorbs a particular waveform or pulse width of an incident wave at the same frequency band, while transmitting others. This unique capability is demonstrated using either ideal sinusoidal waves or Wi‐Fi signals as a more practical waveform in wireless communications. Especially, this Letter shows how the waveform‐selective metasurface interacts with realistic wireless communication signals from the viewpoint of communication characteristics, such as error vector magnitude, bit error rate, and phase error. Thus, the authors’ Letter paves the way for extending the concept of waveform selectivity from a fundamental electromagnetic research field to a more realistic wireless communication field to, for instance mitigate electromagnetic interference occurring at the same frequency band without significantly degrading communication characteristics.
LADA – Latenter Autoimmuner Diabetes im Erwachsenenalter
Authors: Hummel Michael , Füchtenbusch Martin 
Published: October 2019

ZUSAMMENFASSUNGKriterien für die Diagnose eines LADA sind eine Manifestation nach dem 30. Lebensjahr, Nachweis von Insel-Autoantikörpern und die Möglichkeit, mindestens 6 Monate ohne Insulin zu therapieren. Das C-Peptid ist meist niedrig normal bzw. erniedrigt. Die Häufigkeit des LADA bei Patienten mit dem klinischen Phänotyp eines Typ-2-Diabetes liegt bei ca. 10 %. Bei GAD-Antikörper-positivem Befund müssen binnen 6 Jahren 40 % der Patienten im Alter von über 45 Jahren und 70 % der Patienten unter 45 Jahre mit Insulin behandelt werden. Die verfügbare Datenlage erlaubt keine eindeutigen Schlüsse bezüglich der optimalen initialen medikamentösen Therapie des LADA. Sulfonylharnstoffe sind ungünstiger als Insulin. Die Behandlung mit DPP-4-Hemmern scheint bei LADA ähnlich gut wirksam zu sein wie bei Typ-2-Diabetes. Erste Daten zu Dulaglutid sind positiv, sodass auch GLP-1-Analoga eine Therapiealternative sind.
Genetisch bedingter Non-MODY Diabetes mellitus
Authors: Badenhoop Klaus 
Published: October 2019

ZUSAMMENFASSUNGZunehmend wird deutlich, dass ein Diabetes mellitus auch durch monogenetische Störungen bedingt sein kann. Dies ist zum einen durch die erweiterte Liste von seltenen MODY-Formen (MODY: Maturity Onset Diabetes of the Young), aber auch durch andere Gene erklärbar. Diese Übersicht fasst den aktuellen Stand zu erblichen Non-MODY-Diabetesformen sowohl bei Kindern als auch für Diabetesmanifestationen im Erwachsenenalter zusammen. Diese umfassen unter anderem chromosomale Aberrationen, seltene erbliche Störungen der Insulinwirkung und reichen bis zu Diabetes bei zystischer Fibrose, Hämochromatose oder endokrinen Tumoren. Bei den meisten Formen steht die defekte Betazellsekretion im Vordergrund, sodass primär mit Insulin therapiert wird. Im Einzelfall kann empirisch mit anderen Antidiabetika kombiniert werden.
Trend of Cesarean Section Rates and Correlations with Adverse Maternal and Neonatal Outcomes: A Secondary Analysis of Thai Universal Coverage Scheme Data
Authors: Liabsuetrakul Tippawan , Sukmanee Jarawee , Thungthong Jutatip , Lumbiganon Pisake 
Published: October 2019

Abstract
Objectives The main purpose of this article is to estimate the trend and projection of cesarean section rates (CSRs) and explore correlations between CSRs with adverse maternal and perinatal outcomes, namely maternal mortality ratios (MMRs), rates of postpartum hemorrhage (PPH), neonatal mortality rates (NMRs), and birth asphyxia per 1,000 live births across all regions of Thailand.
Study design A secondary analysis of the hospital-based database of pregnant women and newborns under the Thai Universal Coverage Scheme between January 2009 and December 2017 was conducted.
Results Overall annual CSR significantly increased from 23.2% in 2009 to 32.5% in 2017. With the same rate of increase, the CSR of 59.1% was projected by the year 2030 that could be reduced to 30.0% if an annual rate of CS reduction of 1% was assumed using Joinpoint regression. The increasing CSRs were significantly correlated with higher MMRs (r= 0.20, p = 0.03) and birth asphyxia (r= 0.39, p < 0.001). The correlation trends were similar when the analyses were stratified by year in the majority of years. Overall correlations between CSRs and rates of PPH or NMRs were not statistically significant.
Conclusion CSRs in Thailand continuously increased and were correlated with adverse maternal and perinatal outcomes. More effort at the national level to reduce unnecessary CS is urgently required.
Data Reduction Using Lossy Compression for Cosmology and Astrophysics Workflows
Authors: Pulido Jesus , Lukic Zarija , Thorman Paul , Zheng Caixia , Ahrens James , Hamann Bernd 
Published: October 2019

Abstract
This paper concerns the use of compression methods applied to large scientific data. Specifically the paper addresses the effect of lossy compression on approximation error. Computer simulations, experiments and imaging technologies generate terabyte-scale datasets making necessary new approaches for compression coupled with data analysis. Lossless compression techniques compress data with no loss of information, but they generally do not produce a large-enough reduction when compared to lossy compression methods. Lossy multi-resolution compression techniques make it possible to compress large datasets significantly with small numerical error, preserving coherent features and statistical properties needed for analysis. Lossy data compression reduces I/O data transfer cost and makes it possible to store more data at higher temporal resolution. We present results obtained with lossy multi-resolution compression, with a focus on astrophysics datasets. Our results confirm that lossy data compression is capable of preserving data characteristics very well, even at extremely high degrees of compression.