bluuebunny/crossref_metadata_2025_split huggingface dataset:
An unexpected new diploid Hieracium from Europe: Integrative taxonomic approach with a phylogeny of diploid Hieracium taxa
Authors: Mráz Patrik , Filipaş Liviu , Bărbos Marius Ioan , Kadlecová Jana , Paštová Ladislava , Belyayev Alexander , Fehrer Judith 
Published: December 2019

AbstractHieracium s.str. (Asteraceae) is one of the largest angiosperm genera notorious for its taxonomic complexity caused by widespread interspecific hybridization. This process is tightly coupled with polyploidization and apomixis – asexual reproduction by seeds, which has ensured the persistence of otherwise sterile interspecific hybrids. As a result, apomictic polyploid taxa dominate the taxonomic diversity of the genus whilst sexual diploid species are extremely rare and mostly confined to southern Europe. As diploid taxa are assumed to be the parents of apomictic lineages, the discovery of any new diploid species is important for understanding evolutionary processes and diversity patterns in the genus. Here, we describe a new diploid species, Hieracium vranceae, a narrow endemic to the Munţii Vrancei (Vrancea Mountains, Eastern Carpathians, Romania). This taxon with a distinctive morphology and a strong affinity to relic rocky habitats was first collected in 2013. This means that, even in Europe, some regions, like the Eastern Carpathians, are botanically underexplored and might still preserve an undiscovered diversity of plants. Phylogenetic analyses with multiple molecular markers (low‐copy nuclear genes gsh1 and sqs, nuclear ribosomal ETS, four cpDNA loci) applied to all diploid Hieracium species support specific rank for H. vranceae. In contrast, molecular data suggest conspecifity of two pairs of species, H. lucidum/H. cophanense from Sicily and H. naegelianum/H. renatae from the Balkans, which are moreover only weakly differentiated morphologically. Molecular (cpDNA) and cytogenetic (GISH, FISH) analyses furthermore revealed that H. vranceae has been involved as a maternal parent in the origin of an allotriploid apomictic species, H. telekianum, to which H. vranceae contributed one haplome. The other putative parents of H. telekianum are the diploid narrow endemic H. pojoritense and probably H. sparsum s.l. Our data thus stress the importance of interspecific hybridization for an evolutionary shift from sexuality to apomixis in Hieracium. Our findings furthermore highlight the significance of that part of the Carpathians as one of the most important evolutionary/refugial centres of Hieracium and suggest dynamic species ranges at a regional scale allowing physical contact of taxa whose distributional ranges are nowadays completely allopatric.
Information‐theoretic approach in density functional theory and its recent applications to chemical problems
Authors: Rong Chunying , Wang Bin , Zhao Dongbo , Liu Shubin 
Published: December 2019

AbstractUsing simple functionals of the electron density to appreciate and quantify molecular structure and chemical reactivity properties is a recent endeavor in density functional theory (DFT) toward the development of a new chemical reactivity theory. According to the first Hohenberg–Kohn theorem in DFT, the electron density alone should be able to determine any property in the ground state. Exchange and correlation energies are such properties, so are molecular structure and chemical reactivity, and hence they all should accurately be determined by electron density functionals. Quantities such as Shannon entropy, Fisher information, Ghosh–Berkowitz–Parr entropy, information gain, Onicescu information energy, etc., from the information‐theoretic approach are simple electron density functionals, whose analytical forms are exactly known. In this article, we demonstrate their usefulness and validity to quantify regioselectivity, stereoselectivity, and other structure and reactivity properties. We will outline the current understanding of its theoretical framework at first, and then highlight recent applications to chemical problems including isomeric and conformational stability, electrophilicity and nucleophilicity, strong covalent and weak noncovalent interactions, acidity and basicity, aromaticity and antiaromaticity, and numerous other properties. The effort of employing electron density functionals to quantify chemical concepts should open up a new door for us to ultimately develop a chemical reactivity theory using the DFT language.This article is characterized under:Structure and Mechanism > Reaction Mechanisms and CatalysisElectronic Structure Theory > Density Functional TheoryStructure and Mechanism > Molecular StructuresMolecular and Statistical Mechanics > Molecular Interactions
The role of hypoxia-inducible factors in neovascular age-related macular degeneration: a gene therapy perspective
Authors: Mammadzada Parviz , Corredoira Pablo M. , André Helder 
Published: December 2019

AbstractUnderstanding the mechanisms that underlie age-related macular degeneration (AMD) has led to the identification of key molecules. Hypoxia-inducible transcription factors (HIFs) have been associated with choroidal neovascularization and the progression of AMD into the neovascular clinical phenotype (nAMD). HIFs regulate the expression of multiple growth factors and cytokines involved in angiogenesis and inflammation, hallmarks of nAMD. This knowledge has propelled the development of a new group of therapeutic strategies focused on gene therapy. The present review provides an update on current gene therapies in ocular angiogenesis, particularly nAMD, from both basic and clinical perspectives.
Structural genome analysis in cultivated potato taxa
Authors: Kyriakidou Maria , Achakkagari Sai Reddy , Gálvez López José Héctor , Zhu Xinyi , Tang Chen Yu , Tai Helen H. , Anglin Noelle L. , Ellis David , Strömvik Martina V. 
Published: December 2019

AbstractKey messageTwelve potato accessions were selected to represent two principal views on potato taxonomy. The genomes were sequenced and analyzed for structural variation (copy number variation) against three published potato genomes.AbstractThe common potato (Solanum tuberosumL.) is an important staple crop with a highly heterozygous and complex tetraploid genome. The other taxa of cultivated potato contain varying ploidy levels (2X–5X), and structural variations are common in the genomes of these species, likely contributing to the diversification or agronomic traits during domestication. Increased understanding of the genomes and genomic variation will aid in the exploration of novel agronomic traits. Thus, sequencing data from twelve potato landraces, representing the four ploidy levels, were used to identify structural genomic variation compared to the two currently available reference genomes, a double monoploid potato genome and a diploid inbred clone ofS. chacoense. The results of a copy number variation analysis showed that in the majority of the genomes, while the number of deletions is greater than the number of duplications, the number of duplicated genes is greater than the number of deleted ones. Specific regions in the twelve potato genomes have a high density of CNV events. Further, the auxin-induced SAUR genes (involved in abiotic stress), disease resistance genes and the 2-oxoglutarate/Fe(II)-dependent oxygenase superfamily proteins, among others, had increased copy numbers in these sequenced genomes relative to the references.
Electro-fluidic timer for event control in paper-based devices
Authors: Llorella Anna , Navarro-Segarra Marina , Merino-Jiménez Irene , Esquivel Juan Pablo , Sabaté Neus 
Published: December 2019

AbstractIn this paper, we present a simple yet smart electro-fluidic platform that enables automatic time control in a very affordable and simple manner. The system is based on the electric detection of a fluid front when it crosses a particular area of a paper strip. The detection can be used to trigger the sequential activation or deactivation of different electronic modules (heating of molecular diagnostics, time interval detection, or readout of test results) with an accuracy within the range of minutes. The whole system is implemented with a few number of discrete electronic components such as transistors, resistors and capacitors that, if required, can be totally fabricated using printed electronics technology. This platform opens new possible applications for paper-based point-of care (POC) diagnostic devices and enables the possibility of these devices to introduce time control functions without the need for any external instrumentation and human action.
How much does the rainfall temporal resolution affect rainfall thresholds for landslide triggering?
Authors: Gariano Stefano Luigi , Melillo Massimo , Peruccacci Silvia , Brunetti Maria Teresa 
Published: December 2019

AbstractIn many areas of the world, the prediction of rainfall-induced landslides is usually carried out by means of empirical rainfall thresholds. Their definition is complicated by several issues, among which are the evaluation and quantification of diverse uncertainties resulting from data and methods. Threshold effectiveness and reliability strongly depend on the quality and quantity of rainfall measurements and landslide information used as input. In this work, the influence of the temporal resolution of rainfall measurements on the calculation of landslide-triggering rainfall thresholds is evaluated and discussed. For the purpose, hourly rainfall measurements collected by 172 rain gauges and geographical and temporal information on the occurrence of 561 rainfall-induced landslides in Liguria region (northern Italy) in the period 2004–2014 are used. To assess the impact of different temporal resolutions on the thresholds, rainfall measurements are clustered in increasing bins of 1, 3, 6, 12 and 24 h. A comprehensive tool is applied to each dataset to automatically reconstruct the rainfall conditions responsible for the failures and to calculate frequentist cumulated event rainfall–rainfall duration (ED) thresholds. Then, using a quantitative procedure, the calculated ED thresholds are validated. The main finding of the work is that the use of rainfall measurements with different temporal resolutions results in considerable variations of the shape and the validity range of the thresholds. Decreasing the rainfall temporal resolution, thresholds with smaller intercepts, higher slopes, shorter ranges of validity and higher uncertainties are obtained. On the other hand, it seems that the rainfall temporal resolution does not influence the validation procedure and the threshold performance indicators. Overall, the use of rainfall data with coarse temporal resolution causes a systematic underestimation of thresholds at short durations, resulting in relevant drawbacks (e.g. false alarms) if the thresholds are implemented in operational systems for landslide prediction.
Symbiosis extended: exchange of photosynthetic O2 and fungal-respired CO2 mutually power metabolism of lichen symbionts
Authors: ten Veldhuis Marie-Claire , Ananyev Gennady , Dismukes G. Charles 
Published: December 2019

AbstractLichens are a symbiosis between a fungus and one or more photosynthetic microorganisms that enables the symbionts to thrive in places and conditions they could not compete independently. Exchanges of water and sugars between the symbionts are the established mechanisms that support lichen symbiosis. Herein, we present a new linkage between algal photosynthesis and fungal respiration in lichenFlavoparmelia caperatathat extends the physiological nature of symbiotic co-dependent metabolisms, mutually boosting energy conversion rates in both symbionts. Measurements of electron transport by oximetry show that photosynthetic O2is consumed internally by fungal respiration. At low light intensity, very low levels of O2are released, while photosynthetic electron transport from water oxidation is normal as shown by intrinsic chlorophyll variable fluorescence yield (period-4 oscillations in flash-induced Fv/Fm). The rate of algal O2production increases following consecutive series of illumination periods, at low and with limited saturation at high light intensities, in contrast to light saturation in free-living algae. We attribute this effect to arise from the availability of more CO2produced by fungal respiration of photosynthetically generated sugars. We conclude that the lichen symbionts are metabolically coupled by energy conversion through exchange of terminal electron donors and acceptors used in both photosynthesis and fungal respiration. Algal sugars and O2are consumed by the fungal symbiont, while fungal delivered CO2is consumed by the alga.
Design and application of adaptive PID controller based on asynchronous advantage actor–critic learning method
Authors: Sun Qifeng , Du Chengze , Duan Youxiang , Ren Hui , Li Hongqiang 
Published: December 2019

AbstractTo address the problems of the slow convergence and inefficiency in the existing adaptive PID controllers, we propose a new adaptive PID controller using the asynchronous advantage actor–critic (A3C) algorithm. Firstly, the controller can train the multiple agents of the actor–critic structures in parallel exploiting the multi-thread asynchronous learning characteristics of the A3C structure. Secondly, in order to achieve the best control effect, each agent uses a multilayer neural network to approach the strategy function and value function to search the best parameter-tuning strategy in continuous action space. The simulation results indicate that our proposed controller can achieve the fast convergence and strong adaptability compared with conventional controllers.
Multifractal analysis of human canine teeth at nano scale: atomic force microscopy studies
Authors: Solaymani Shahram , Ţălu Ştefan , Ghoranneviss Mahmood , Elahi Seyed Mohammad , Shafiekhani Azizollah , Hantehzadeh Mohammadreza , Nezafat Negin Beryani 
Published: December 2019

AbstractThe aim of the present study is to explore the 3-D micromorphology of human canine teeth materials using multifractal analysis through atomic force microscopy (AFM). The 3-D surfaces of ten extracted canine teeth of a group of 40 year old men were studied (enamel, inter enamel, inter dentin, and cementum) by AFM images in tapping mode and on square areas of 1 μm × 1 μm (512 × 512 points). The AFM images and surface multifractal analysis confirm the dependency of surface micromorphology to their structure–property of these materials across the length scales of the teeth structural architecture. Surface statistical parameters and hence, multifractal approach have been considered as reliable and sensitive tools for quantifying the 3-D surface microtexture changes of human canine teeth materials. The surface of inter dentin had the most irregular topography (the width spectrum Δα = 2.8361, value bigger than all the other Δα sample values), while the most regular topography (the width spectrum Δα = 2.6804, value lower than all the other sample values) was found in cementum. It has been concluded that multifractal analyses can be used as mathematical tools to explore the 3-D micromorphology of human canine teeth materials.
Particulars in Phaedo, 95e — 107a
Authors: White F. C. 
Published: December 2019

In this paper there are two claims that I wish to defend. One is that in Socrates’ much discussed “causal” theory concrete particulars are more central than Forms. The other is that these concrete particulars are held by Plato to be not simply bundles of characteristics, not mere meeting-points of Forms, but independent individuals, existing in their own right.It will not, I believe, be questioned that from one point of view the prime concern of the Phaedo is with concrete particulars; not with Forms, characteristics, or anything else. For, the overriding aim of the dialogue is, surely, to persuade us each to care for his own soul; it is for this reason that arguments are put forward to show that souls are indestructible. But there is no doubt that these souls are particulars.
OP14 Progress In Use Of Telerehabilitation For Persons With COPD
Authors: Hailey David , Roine Risto 
Published: December 2019

IntroductionTelerehabilitation shows promise in many fields though strong evidence of benefit has been limited. We reviewed progress in the use of telerehabilitation for persons with chronic obstructive pulmonary disease (COPD). A challenge in caring for persons with this condition is the ability to achieve high levels of patient participation and compliance with rehabilitation processes.MethodsRelevant publications were identified through literature searches from November 2009 to May 2018. We selected those that described studies of telerehabilitation in the management of COPD and reported clinical or administrative outcomes. Study quality was assessed using an approach that considers both study performance and study design. Judgments were made on whether the telerehabilitation application had been successful, if reported outcomes were clinically significant, and if further data were needed to establish the application as suitable for routine use.ResultsTwenty-five publications, on 26 studies, were selected. Twelve were of high or good quality. In 11 studies the telemedicine application was successful. Nine studies had unsuccessful applications, and for six studies success was unclear. Further data before routine use would be required or desirable for all successful applications. In many studies there were difficulties associated with availability of skilled mentors, motivational support for patients and access to reliable remote monitoring and communication technology.ConclusionsVarious types of telerehabilitation are potentially helpful in the management of COPD. Availability and access to these technologies should improve. However, in management of this clinically challenging condition their use must be linked to suitable training and education of patients with COPD and timely support for them from healthcare professionals.
OP30 Impact Of Patient Reported Outcomes Data On Health Technology Assessments Of Acute Myeloid Leukemia
Authors: Shah Manasee , Ernst Rianne , Holmstrom Stefan , van Hooijdonk Inge 
Published: December 2019

IntroductionPatient-reported outcomes (PRO) data are important in understanding patients’ experience of disease and treatment; however, PRO data are not universally collected or consistently included as part of a Health Technology Assessment (HTA) submission. Additionally, the HTA bodies’ response to PRO data vary, making the impact unclear. To understand the impact of PRO data on reimbursement decisions for Acute Myeloid Leukemia (AML) indications, an in-depth analysis of HTA bodies’ appraisals of AML and analogous indications was conducted.MethodsThis analysis was conducted using IQVIA's HTA Accelerator, which contains HTA appraisals from ≥100 HTA agencies in thirty-nine countries. Included in the analysis were single-technology assessments (original submissions, resubmissions, extensions of original indications, and renewals); relevant regulatory approvals and pivotal trials were also analyzed.ResultsOf the 185 AML appraisals from sixteen HTA bodies, 66 (36%) included PRO data. Within these, thirteen different PRO instruments were identified, none of which have been validated in patients with AML. For seven of twenty in-scope products, PRO evidence positively impacted ≥1 of the HTA decisions. Although the same HTA bodies (i.e., Scottish Medicines Consortium, pan-Canadian Oncology Drug Review, and the National Institute of Health and Care Excellence) generally accepted the PRO evidence, others were critical of the evidence (i.e., Haute Autorité de Santé and the Institut für Qualität und Wirtschaftlichkeit im Gesundheitswesen). The most common concerns raised by the HTA bodies regarding the PRO evidence included trial design and low patient response rate.ConclusionsOf the products that included PRO evidence in their HTA submissions, 35% received positive feedback from ≥1 HTA body on their submitted PRO evidence. Attention to PRO data collection is key to demonstrate the value of AML products to HTA bodies. Without these data, a clear gap in the understanding of patients’ experience is evident.
OP54 Monitoring Evidence On Overall Survival Benefits Of Anti-Cancer Drugs
Authors: Grössmann Nicole , Robausch Martin , Rosian Katharina , Wild Claudia , Simon Judit 
Published: December 2019

IntroductionThe introduction of fast-track licensing strategies increases the approval of anti-cancer drugs with ambiguous benefit-risk profiles. Thus, in many instances there is lacking evidence about overall survival (OS) at the time of marketing authorisation. Our objective was to monitor and characterise therapies with ambiguous benefit-risk profiles and identify any post-approval updates on median OS after at least three years of approval by the European Medicines Agency (EMA).MethodsWe included all originator anti-cancer drugs with initially ambiguous benefit-risk profiles that received marketing authorization from the EMA between 1 Jan 2009 and 31 May 2015. Our monitoring timeframe was at least three years after EMA-approval. To identify study updates, the following three sources were included: clinicaltrials.gov, European Public Assessment Reports (EPARs), and PubMed.ResultsIn total, we identified 102 eligible approval studies. Out of these, a negative difference in median OS or no information was available in forty-three (42.2%) instances. During monitoring, eleven updates with accessible information on median OS could be identified. Including monitoring results, there are still thirty-two remaining therapies (31.4%) where no or negative information (n = 27 [26.5%] and n = 5 [4.9%], respectively) regarding median OS was present at least three years after EMA approval.ConclusionsOne-third of oncology drugs with ambiguous benefit-risk profiles failed to demonstrate a survival benefit even several years following marketing authorization. Systematic and transparent post-approval monitoring mechanisms will be of high relevance to assure a clinically relevant patient benefit, since the trend towards faster access to medicines with uncertain benefit is increasing rather than declining.