bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of CENPO&nbsp;Expression in the Progression of Non-Small Cell Lung Cancer
Authors: Yang Yanlong , Chen Xiaobo , Sun Hongwen , Yu Qinghe , Yin Fang , Hu Zaoxiu , Sun Yongmeng , Pu Lisha , Zhu Xinming , Li Shen , Zhao Yunping 
Published: August 2020

Abstract
Background: The role of Centromere protein O (CENPO) in the development of non-small cell lung cancer (NSCLC) is unknown. This study aimed to investigate the potential roleof CENPO in the development of NSCLCMethods: The expression level of CENPO was investigated in both tissues and cell-lines. Celigo cell counting assay, wound-healing assay, Transwell assay and Flow cytometry was used to explore the effect of CENPO on proliferation, migration, invasion, apoptosis and cell cycle of NSCLC. The potential mechanism of CENPO was explored by Human Apoptosis Antibody, also, western blot was conducted to detect the expression of PI3K/Akt/mTOR pathway and cell-cycle related protein (mTOR, P-mTOR, CDK1, CDK6 and PIK3CA). Besides, the effect of CENPO on the growth of NSCLC solid tumors was demonstrated in vivo.Results: Our study suggested CENPO gene overexpression in NSCLC. Reduced CENPO expression substantially decreased the proliferation, migration and invasion ability, and promoting apoptosis and induces cell cycle arrest of NSCLC cell-lines. Preliminary mechanism research suggested reduced CENPO could active apoptosis pathway, suppressing PI3K/AKT/mTOR pathway and down-regulation cell-cycle related proteins.Conclusion: CENPO was up-regulated in NSCLC and played an important role in promoting the progress of NSCLC.
Analysis of Correlation Between Whole Exome Sequencing and Ultrasound Examinationin Prenatal Diagnosis of Fetal Skeletal Dysplasia&nbsp;
Authors: Tian Yuan , Yuan Erfeng , Gao Jinshuang , Guo Yaqing , Yu Haiyang , Zhang Linlin , Zhang Xiao’an , Zhao Xin , Yuan Enwu , Cui Shihong 
Published: August 2020

Abstract
Background:Fetal skeletal dysplasia is a disease that is difficult to distinguish these types of diseases during the fetal period. Due to the difficulty of fetal ultrasound diagnosis, the severity of fetal skeletal dysplasia is extremely difficult to assess.Methods: 79 fetal samples of skeletal dysplasia from the third affiliated hospital of Zhengzhou University, China from August 2018 to April 2020, which had undergone prenatal whole exome sequencing(WES) were analyzed and the results of whole-exome sequencing and fetal ultrasound test results were compared. Results:We find that the fetal short limb phenotype found in the range of FL<-4.0SD or HL<-4.0SD through ultrasound test is closely related to FGFR3 gene mutation , and the correlation is stronger when accompanied by macrocephaly. We also find that the fetal limb curved phenotype is closely related to COL1A1 gene mutation. At the same time, we find that nasal dysplasia during fetal period is also a common phenotype of abnormal results detected by whole exome sequencing.Conclusions: Our research shows that WES has different detection rates for various skeletal abnormalities according to the different types of ultrasound detection results, which provides a meaningful guidance for clinical diagnosis of fetal skeletal dysplasia.
Relationship of Red Cell Index&nbsp;with the Severity of&nbsp;COPD
Authors: Huang Yiben , Wang Jianing , Shen Jiamin , Ding Keke , Huang Honghao , Yang Chenguang , Fu Fangyi , Hu Binbin , Pan Wenjing , Jia Jiaying , Tong Wangyong , Zhou Xinbo , Zhang Xiaodiao 
Published: August 2020

Abstract
Background: Chronic obstructive pulmonary disease (COPD) has always attracted attention due to its high prevalence and high mortality. How to predict and diagnose COPD and assess the severity of the disease is our top priority. We aimed to evaluate the association between red cell index (RCI) and the severity of COPD, and compare predictive value among RCI, neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) of indicating the severity of COPD.Methods: A total of 207 participants were recruited (100 COPD patients and 107 healthy controls). COPD patients were divided into two groups according to receiver operating characteristics (ROC) curves cut-off value of RCI (RCI < 1.75, n = 54; RCI > 1.75, n = 46). Pearson’s correlation test, logistic regression analysis and other tests were performed.Results: Compared with low RCI group, the forced expiration volume in 1 second (FEV1) and FEV1 in percent of the predicted value (FEV1%) of high RCI group decreased (p = 0.016, p = 0.001). There is a negative correlation between RCI and FEV1% (r = -0.302, p = 0.004), while no correlation between FEV1% and NLR and PLR. RCI’s ability to predict Global Initiative for Chronic Obstructive Lung Disease classification, is better than NLR and PLR, with a cut of 1.75, specificity of 85.2%, sensitivity of 57.6% and area under the curve (AUC) of 0.729 (p = 0.001). Multivariate logistic regression analysis proved RCI was an independent factor affecting lung function in COPD patients (odds ratio [OR] = 4.27, 95%CI: 1.57 - 11.63, p = 0.004). Conclusion: RCI is a novel biomarker that can better assess respiratory function and severity of COPD than NLR and PLR. Higher RCI is independently related to deterioration of respiratory function.
Two-layer duct-to-mucosa pancreaticojejunostomy with a half purse-string suture: A novel anastomosis technique for soft pancreas
Authors: Meng Yao , Jiang Yabo , Jing Wei , Zheng Kailian , Cheng Peng , Zhang Yijie , Jin Gang , He Tianlin 
Published: August 2020

Abstract
Background: Pancreaticoduodenectomy (PD) is a routine method in pancreatic surgery. To date, postoperative pancreatic fistula (POPF) remains the most common complication and is also the major cause to death after pancreaticoduodenectomy. In order to reduce the incidence of POPF, we established a new anastomosis technique where we use a half purse-string suture on the basis of two-layer duct-to-mucosa pancreaticojejunostomy (PJ) technique and also assessed the effectiveness and safety of this approach in this study.Methods: To evaluate this new approach, 80 patients who received the new PJ technique were included in this study during 2017–2018. Meanwhile, 195 patients who underwent traditional duct-to-mucosa PJ were collected. We also introduced this new surgical approach in detail and analyzed various risk factors for postoperative complications to verify its safety and advantages.Results: First of all, there were no significant differences in patients' preoperative characteristics. Besides, patients in the new PJ group received a less operative time (175.2±45.8 vs. 161.3±41.0, p=0.022) while no differences were found in blood loss, length of hospital stay and cost. The incidence of POPF in the new PJ group was 19%, which was much lower than those in the traditional PJ group (36%) (p=0.007). More importantly, a much lower incidence of grades B POPF (14% vs. 4%, p=0.026) was found in the new technology group, which would clinically benefit patients a lot. Univariate and multivariate regression analysis also verified that this new PJ procedure was effective to improve postoperative POPF.Conclusions: Results demonstrate that this new technique is easy accomplished, safe and effective compared to traditional approach, which showed satisfactory outcomes especially at resulting in a lower POPF incidence.
Comparison of Short-Term Efficacy of Total Knee Arthroplasty Versus Arthroscopy in Chinese Patients with Knee Osteoarthritis
Authors: Jia Xiaolin , Cai San , Hu Wei , Gan Qiang , Zhou Mingquan 
Published: August 2020

Abstract
Background: The purpose of this study was to compare the improvement of knee function in patients with knee osteoarthritis who underwent total knee arthroplasty and arthroscopy in China, and to provide a scientific basis for the application of clinical total knee arthroplasty in knee osteoarthritis.Methods: A total of 160 patients with knee osteoarthritis who were admitted to Chinese hospital from January 2017 to December 2018 were studied. They were divided into experimental group and control group according to their willingness of treatment. The control group was treated with arthroscopy and the experimental group was treated with total knee arthroplasty. All patients were followed for a period of 6 months. The knee joint function score (HSS), visual analog scale (VAS), and anxiety self-assessment scale (SAS) scores before and after surgery were compared between the two groups. Results: The proportion of "excellent or good" in the efficacy of the experimental group (91.25%) was higher than that of the control group (72.50%), and the difference was statistically significant (χ2=9.476, P<0.05). The HSS score of the experimental group was higher than that of the control group (P<0.05), while the VAS and SAS scores were lower than those of the control group (P<0.05). The scores of various SF-36 scales in the experimental group were higher than those in the control group after operation (P<0.05).Conclusions: Total knee arthroplasty was considered effective in treating patients with knee osteoarthritis that meet the indications, and is beneficial to improve knee function and reduce pain in patients. The surgical treatment also reduced the level of anxiety and effectively improve the quality of life of patients. Further investigation of its clinical application on treatment of knee osteoarthritis is warranted.
Sputum Microbiota in Coal Workers Diagnosed With Pneumoconiosis as Revealed by 16S Metagenomic Sequencing
Authors: Druzhinin Vladimir , Matskova Liudmila , Demenkov Pavel , Baranova Elizaveta , Volobaev Valentin , Minina Varvara , Larionov Alexey , Paradnikova Snezhana 
Published: August 2020

Abstract
Objectives: The microbiome of sputum from former and active coal miners diagnosed with coal worker’s pneumoconiosis (CWP) as compared to healthy controls Methods: Next Generation Sequencing of bacterial 16S rRNA genes obtained from the sputum of CWP subjects.Results: Differences were detected between the sputum microbiomes from the healthy and CWP subjects. We noted a significant decrease in Bacteroidetes and an increase in the level of Proteobacteria.Conclusions: The microbiomes found in sputum from CWP subjects are enriched in bacterial species previously reported to induce pro-inflammatory responses. The profile of the microbiomes correlated mainly to the occupational activity and not to the age of the coal miners.
The Changes of Serum Lipids in Idiopathic Epiretinal Macular Membranes
Authors: Feng Liguo , Chen Ying , Xu Yesheng , Xie Wenjia , Sun Yi , Wang Binghong , Yao Yu-Feng 
Published: August 2020

Abstract
Purpose: To describe the changes of serum lipids in patients with idiopathic epiretinal macular membranes (iERMs). Methods: A retrospective, observational case control study was performed January 2015 and December 2019 in our unit. A total of 57 subjects with idiopathic epiretinal macular membranes were collected in the study. Senile cataract patients with age matched during hospitalizations were randomly collected as control group. iERMs were diagnosed based on OCT images. Comprehensive ophthalmologic examinations and Systemic examinations were performed. The changes of serum lipids were analyzed. Results: The iERMs was common in female (71.9%). Compared to control group, Serum triglycerides were higher in iERMs group (1.54 ± 0.66 vs 1.21 ± 0.50, P < 0.01). There was significant difference in BMI with iERM (23.68 ± 2.86 vs 22.50 ± 2.71, P = 0.03). After subjects stratified according whether with hypertension diseases, there was still statistical difference in serum triglycerides (P < 0.05). Conclusions: Serum triglycerides and BMI are elevated in patients with iERMs. It suggests that obese people seem to be prone to iERMs. Hyperlipemia seems to play a role in the formation of iERMs.
The Outbreak of COVID-19 Pandemic and Its Impact on Stock Market Volatility: Evidence from a Worst-affected Economy
Authors: Bora Debakshi , Basistha Daisy 
Published: August 2020

Abstract
The outbreak of COVID-19 has affected the entire global financial market in an unprecedented way. Due to the disruptions that emerged in the global market; the financial market of India also reacted to the pandemic and witnessed sharp volatility. Given the COVID-19 situation, this paper empirically investigates the impact of COVID-19 on the Indian stock market. Using daily closing prices of indices such as Nifty and Sensex, this study examines the volatility of these indices over the period 3rd September 2019 to 10th July 2020. Further, the study has attempted to make a comparative analysis of the return of the stock market in pre-COVID-19 and during the COVID-19 situation. GARCH model is used to capture the volatility of the indices. Findings reveal that the stock market in India has experienced volatility during the pandemic period. While comparing the results with that of the pre-COVID-19 period, we find that return on the indices is higher in the pre-COVID-19 period than during COVID-19. The return of both the stock market reached the bottom line during the first lockdown period, which is from24th March to 6th April.
CORO6 Promotes Cell Growth and Invasion of Clear Cell Renal Cell Carcinoma via Activation of WNT Signaling
Authors: Wang Xinjun , Xiao Yiming , Yan Zhijian , Luo Guangcheng 
Published: August 2020

Abstract
BackgroundRenal cell carcinoma (RCC) constitutes the most lethal type of genitourinary cancer. Understanding of RCC tumor biology helps to identify novel targets and develop directed treatments for patients with this type of cancer. MethodsBioinformatical Analysis of The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma dataset. Western blotting and qPCR were used to examine the expression levels of interested genes. Flow cytometry, MTT, BrdU staining, wound healing assay and transwell invasion assay were applied to determine the biological functions of CORO6 in ccRCC cells. The in vivo effect of CORO6 on ccRCC development was validated with xenografted mouse model.ResultsAnalysis from both The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma dataset and our RCC samples demonstrated that the expression level of CORO6 was significantly higher in RCC patients than in normal kidney tissues, and its level was highly associated with tumor stage and grade. Importantly, CORO6 expression level was an independent predictor of tumor metastasis and overall survival in RCC patients. Our cell line data also confirmed that CORO6 knockdown could suppress RCC cell growth as well as cell migration and invasion. The depletion of CORO6 led to cell cycle arrest at the G0/G1 phase and caused cell apoptosis. Further, mechanistic dissection showed that CORO6 mediated RCC cell growth, and cell invasion relied on WNT signaling. Moreover, the in vivo data suggested that CORO6 knockdown indeed suppressed RCC tumor growth. ConclusionsOverall, our study defines the oncogenic role of CORO6 in RCC progression and provides a rationale for developing CORO6-targeted therapies for improved treatment of RCC patients.
Effects of Temperature and Aeration on The Dissolved Oxygen (DO) Values in Freshwater Using Simple Water Bath Reactor: A Brief Report
Authors: Febiyanto Febiyanto 
Published: August 2020

<em>This work investigated the effect of temperature and aeration treatments on the dissolved oxygen (DO) values in freshwater. The study was carried out using a simple water bath reactor in room temperature condition. Based on the results, freshwater sample without the aeration and temperature treatments (control) indicates a fluctuating profile on which the obtained values of DO were 4.21-4.98 mg/L at T<sub>average </sub>= 27.7 °C. However, the aeration treatment was slightly able to enhance the DO value up to 8.12 mg/L from the initial concentration of 3.88 mg/L at T<sub>average </sub>= 27.4 °C. Compared to the aeration treatment, the addition of temperature treatment has extremely increased the DO value up to 21 mg/L from 6.6 mg/L (T<sub>0 minutes</sub> = 27 °C) for 20 minutes of DO observation (T<sub>20 minutes</sub> = 12.4 °C). Hence, this brief report suggests that the addition of temperature treatment gave a significant effect on the DO value in freshwater than the aeration treatment and control.</em>
Modified particle swarm algorithm for the optimal water allocation of reservoir
Authors: Gong Zhihao , Cheng Jilin , Gong Yi , Wang Liang , Wei Cong 
Published: August 2020

Abstract
At present, meta-heuristic algorithms are the most popular methods for the optimization of the operations of reservoirs. In order to avoid inappropriate solutions, i.e. spills occurring when the reservoir is not full, a modified method is proposed that can adjust the trajectories of the particles, using the particle swarm algorithm, according to the operation rule of the reservoir. The method was tested in a case study, and was compared to two commonly used methods for generating particle position vectors. These included the direct method, which uses water supply and water spills as the iteration variables, and the indirect method, which uses water storages (water levels) as the iteration variables. The results showed that the three methods could achieve similar solutions at the 75% probability of exceedance. There was no difference in the convergence speeds or the final objective function values of the three models. However, at the 50% probability of exceedance, the modified method produced results that followed the operation rule of the reservoir, whereas the other two methods could lead to inappropriate water spills. This new method may provide a reference for other meta-heuristic algorithms in models of the optimal operation of reservoirs.
Characterisation of floc size, effective density and sedimentation under various flocculation mechanisms
Authors: Fan Yuping , Ma Xiaomin , Dong Xianshu , Feng Zeyu , Dong Yingdi 
Published: August 2020

Abstract
Floc structure plays an important role in the separation of coal wastewater. In this study, a camera-based method is used to evaluate quantitatively the structural characteristics of flocs generated by different coagulants and flocculants. The correlations between particle size, settlement velocity and effective density of coal tailings flocs are analysed. The results show that the statistical settling velocity increases linearly with floc size, while the effective density decreases with increase in floc size. Different flocculation mechanisms lead to diverse growth abilities of flocs. When the flocculant is used alone, the quality of the flocs generated by the flocculants, cationic polyacrylamide (CPAM) and non-ionic polyacrylamide (NPAM), is better than that generated by anionic polyacrylamide (APAM). However, the combination of trivalent cations and APAM yields a much better effect than that obtained using CPAM and NPAM. Flocs become larger and more compact when treated with a coagulant combined with a flocculant.
Evaluation of General Health Status in Diabetic Patients Using Short Form Health Survey (SF-36)
Authors: Al-Ibrahimy Ali Shlash , Rabea Ihsan Salah 
Published: August 2020

Objectives::The aim of this study is to assess the general health of diabetic type 2 patients by using SF-36 and also to find if there was an association between the scores of eight domains of this tool with disease-specific and demographic variables.Methods::cross-sectional study was conducted to evaluate the general health of patients with diabetes mellitus type 2. The participants of the present study were randomly selected from rural and urban areas. The diabetic patients visiting community pharmacies in these areas were invited to participate in this study after explaining the goal of the study. A self-reported questionnaire in the Arabic version of the medical outcome survey, the Short-Form (36-item), was conducted. However, uneducated patients were interviewed by trained pharmacists in the community pharmacies.Results and Discussion:Two hundred confirmed DM patients were enrolled in this study with a mean age of (50.65 ± 8.914 years). 142 (71%) were male and the remaining 58 (29%) were female. The scores of all domains of SF-36 were significantly lower (p < 0.05) in female patients in comparison to that recorded in men. In addition, diabetic patients aged more than 50 years showed significantly lower scores of most domains of SF-36 (p < 0.05) except for emotional well-being (p > 0.05). The multivariate linear regression analysis demonstrated that gender, age, and treatment type were independent of health status based on the SF-36 survey, while emotional well-being, social functioning, and pain were exceptional.Conclusion::The outcomes of this study showed a negative correlation between diabetes mellitus and the health status as measured by SF-36. Furthermore, excluding emotional well-being, social functioning, and pain domains, the other parameters of gender, age, and treatment type showed a significant correlation with health status.
Administered Pediatric Radiopharmaceutical Doses at a Tertiary Hospital in South Africa: A Comparison with Corresponding Activities Based on North American Consensus Guidelines and Administration of Radioactive Substances Advisory Committee Guidelines
Authors: Nyathi Mpumelelo 
Published: August 2020

Background:
Diagnostic nuclear medicine reveals physiological processes in vivo, facilitating
early detection of disease prior to anatomical changes. However, in pediatric studies, the selection
of appropriate dosing guidelines is challenging. Administration of Radioactive Substances
Advisory Committee (ARSAC) and North American Consensus (NAC) guidelines are extensively
used.

Objective:
To determine appropriate pediatric dosing guideline for a South African Tertiary Hospital
(SATH).

Methods:
A combination of retrospective and empirical studies was conducted. Age, weight, name
of the nuclear medicine study and administered activities were extracted from archived pediatric patients’
files in a SATH who were attended from 2012-2015. To increase the sample size when calculating
would be administered activities based on ARSAC and NAC guidelines, weights for sixty
pediatric patients (empirical data) from the commonly conducted nuclear medicine studies were
used.

Results:
The most commonly performed nuclear medicine studies at a SATH were bone scans,
99mTc-HIDA scans, renal scans, thyroid scans, MIBG scans and gastroesophageal reflux scans. The
mean pediatric administered radiopharmaceutical activities based on SATH, ARSAC and NAC
guidelines were; bone scans (57.7, 15.2 and 10.0 MBq/kg), 99mTc-HIDA scans (13.7, 5.0 and 3.6
MBq/kg), renal scans (13.9, 3.4 and 7.8 MBq/kg), thyroid scans (7.0, 2.6 and 1.5 MBq/kg), MIBG
scans (15.5, 15.1 and 7.7 MBq/kg) and gastroesophageal reflux scans (2.1, 1.9 and 1.7 MBq/kg).
High variability of Administered Radiopharmaceutical Activities (ARAs) was observed for SATH
guidelines compared to ARSAC and NAC guidelines.

Conclusion:
NAC guidelines are recommended for dosing pediatric patients at SATH. These guidelines
will certainly reduce pediatric doses, which are currently high.