bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intestinal Parasitic Infection and Its Associated Factors among Food Handlers in Ginnir Town, Bale Zone, Southeast, Ethiopia
Authors: Negero Asfaw , Benti Tomas , Taye Solomon 
Published: August 2020

Abstract
Background: Intestinal parasitic infections are the most common infections in the developing world. Globally, out of the 1.5 billion annual cases of diarrhea, about 70% or 1.05 billion cases are thought to be caused by biological contamination of food. In Ethiopia, due to poor environmental sanitation, low awareness about personal hygiene, lack of pure potable water, and improper faecal disposal, intestinal parasitic infections are highly prevalent. Thus, the aim of this study was to determine the prevalence and associated factors of intestinal parasitosis among food handlers working in food and drinking establishments in Ginnir town, southeast, Ethiopia.Methods: Cross-sectional study design was employed from May 1 to May 29, 2014 in food and drinking establishments in Ginnir town. A total of 348 food handlers were enrolled and stool specimens were collected, and examined using direct saline and formol-ether concentration methods. Bivariable and multivariable logistic regression analysis was performed. A P-value <0.05 was considered as an indicator of statistical significance.Results: The overall prevalence of intestinal parasitosis among food handlers was 48/348 (13.8%) [95%, CI=6.8-20.6]. Entamoeba histolytica/dispar 23/348 (6.6%) followed by Giardia lamblia 16/348 (4.6%), Ascaris lumbricoides 7/348 (2.0%) and Strongyloides stercolaris 2/348 (0.6%) were the detected parasites. Moreover, amongst positives for intestinal parasites, comparable proportions of parasites were detected in males (52%) and females (48%). Habit of eating raw/under cooked vegetables (p=0.00; aOR=2.60; 95%, CI=1.94-8.67), hand washing habit before eating (p=0.03; aOR=1.22; 95%, CI=1.01-3.89), hand washing habit after visiting toilet (p=0.01; aOR=2.85; 95%, CI=2.06-8.31), hand washing habit before food preparation (p=0.02; aOR=1.85; 95%, CI=1.27-4.34) and poor personal hygiene (p=0.00; aOR=3.39; 95%, CI=1.51-10.23) were factors associated with intestinal parasitic infections.Conclusions: The prevalence of intestinal parasitosis among food handlers was relatively low. Though the prevalence was low; regular medical check-up, training and health education on safe food handling and processing should be given to all food handlers.
Social Determinants of Mental Health Care Systems: Intensive Community Based Care in The Veterans Health Administration
Authors: Bhalla Ish P , Stefanovics Elina A , Rosenheck Robert A 
Published: August 2020

Abstract
Background: Since deinstitutionalization in the 1950s-1970s, public mental health care has changed its focus from asylums to general hospitals, outpatient clinics and specialized community-based programs addressing both clinical and social determinants of mental health. Analysis of the place of community-based programs within a comprehensive health system such as the Veterans Health Administration (VHA) may illuminate the role of social forces in shaping contemporary public mental health systems. Methods: National VHA administrative data were used to compare veterans who exclusively received outpatient clinic care to those receiving four types of specialized community-based services, addressing: 1) functional disabilities from severe mental illness (SMI), 2) justice system involvement, 3) homelessness, and 4) vocational rehabilitation. Bivariate comparisons and multinomial logistic regression analyses compared groups on demographics, diagnoses, service use, and psychiatric prescription fills. Results: An hierarchical classification of 1,386,487 Veterans who received specialty mental health services from VHA in Fiscal Year 2012, showed 1,134,977 (81.8%) were seen exclusively in outpatient clinics; 27,931 (2.0 %) received intensive SMI-related services; 42,985 (3.1%) criminal justice services; 160,273 (11.6%) specialized homelessness services; and 20,921 (1.5%) vocational services. Compared to those seen only in clinics, veterans in the four community treatment groups were more likely to be black, diagnosed with HIV and hepatitis, had more numerous substance use diagnoses and made far more extensive use of mental health outpatient and inpatient care. Conclusions: Almost one-fifth of VHA mental health patients receive community-based services prominantly addressing major social determinants of health and multimorbid substance use disorders.
OTX1 exerts an oncogenic role and is negatively regulated by miR129-5p in laryngeal squamous cell carcinoma
Authors: Tu Xiu-Ping , Li Hao , Chen Liang-Si , Luo Xiao-Ning , Lu Zhong-Ming , Zhang Si-Yi , Chen Shao-Hua 
Published: August 2020

Abstract
Background Orthodenticle homeobox 1 (OTX1) is a transcription factor that plays an important role in various human cancers. However, the function of OTX1 in laryngeal squamous cell carcinoma (LSCC) is largely unknown. We aimed to explore the roles of OTX1 in LSCC and its possible molecular mechanism.Methods The expression levels of OTX1 were assessed in LSCC cell lines and tissue samples. We further examined the effect of OTX1 on LSCC progression. The upstream regulator of OTX1 was identified using a computer algorithm and confirmed experimentally. Results OTX1 was highly expressed in 70.7% (70/99) of LSCC tissue samples. The OTX1 expression in LSCC was significantly correlated with lymph node metastasis. High OTX1 expression in patients with LSCC was correlated with poor prognosis. Knockdown of OTX1 inhibited proliferation, colony formation, migration and invasion in LSCC cells. Knockdown of OTX1 inhibited tumor growth in a xenograft mouse model. Mechanistically, OTX1 might act as a direct target of miR-129-5p. OTX1 enhanced tumorigenicity and tumor growth both in vitro and in vivo. Conclusions Our findings support that OTX1 is an oncogene in LSCC tumorigenesis and progression. Furthermore, OTX1 is a direct target of miR-129-5p in LSCC cells. Taken together, OTX1 is a promising diagnostic and therapeutic marker for LSCC.
Comparison of Ultrasound-assisted and Pure Fluoroscopy-guided Extracorporeal Shockwave Lithotripsy for Renal Stones
Authors: Chang Tsung-Hsin , Lin Wun-Rong , Tsai Wei-Kung , Chiang Pai-Kai , Chen Marcelo , Tseng Jen-Shu , Chiu Allen W 
Published: August 2020

Abstract
Background: In this study, we aimed to compare the efficacy and clinical outcomes of shock wave lithotripsy (SWL) for patients with renal stones using pure fluoroscopy (FS) or ultrasound-assisted (USa) localization with two lithotripters.Methods: We retrospectively identified 425 patients with renal calculi who underwent SWL with either a LiteMed LM-9200 ELMA lithotripter (209 cases), which combined ultrasound and fluoroscopic stone targeting or a Medispec EM-1000 lithotripter machine (216 cases), which used fluoroscopy for stone localization and tracking. The patient demographic data, stone-free rates, stone disintegration rates, retreatment rates and complication rates were analyzed.Results: The USa group had a significantly higher overall stone-free rate (43.6 vs. 28.2%, p<0.001) and stone disintegration rate (85.6 vs. 64.3%, p<0.001), as well as a significantly lower retreatment rate (14.8 vs. 35.6%, p<0.001) and complication rate (1.9 vs. 5.5%, p=0.031) compared with the FS group. This superiority remained significant in the stone size <1cm stratified group. In the stone size >1cm group, the stone-free rate (32.4 vs. 17.8%, p=0.028), disintegration rate (89.2 vs. 54.8%, p=0.031) and retreatment rate (21.6 vs. 53.4%, p<0.001) were still significantly better in the USa group, however there was no significant difference in the complication rate. The most common complication was post-SWL-related flank pain.Conclusion: Extracorporeal SWL using ultrasound combined with fluoroscopy for stone localization and real-time tracking, provides higher stone-free rates and stone disintegration rates, as well as lower retreatment rates and fewer complications.
Psychosocial Support Interventions for Improved Adherence and Retention in ART care for Young People Living with HIV: A Scoping Review
Authors: Okonji Emeka Francis , Mukumbang Ferdinand C. , Orth Zaida , Vickerman-Delport Shelley A. , Wyk Brian Van 
Published: August 2020

Abstract
Background: Mental health disorders such as high levels of anxiety, isolation, depression and suicide ideation reported among young people living with HIV (10-24; YPLWH) contribute significantly to poor medication adherence and retention in care. While there is evidence supporting the role of psychosocial support interventions in promoting adherence and retention in antiretroviral treatment (ART) among adults living with HIV, there is little evidence on the role of psychosocial support on medication adherence among YPLWH . This scoping review was designed to identify and classify the types and effects of psychosocial support interventions designed to improve adherence and retention in ART among adolescents and young people living with HIV globally. Method: We searched six electronic databases (i.e., Scopus, Pubmed and EBSCOHost (Academic Search Premier, CINAHL, Psycarticles and Medline). Six relevant articles published between 2011 and 2019 met our inclusion criteria. We extracted information relevant to the nature and outcomes of the reported interventions using thematic content analysis informed by the Population, Intervention, comparison, outcome, and time (PICOT) framework. Results: Four distinctive treatment modalities that focused on improving ART adherence and retention in care were identified: individual counselling, support groups, family-centered services, and treatment supporters. Conclusion: There is a dearth of psychosocial support interventions to improve adherence and retention in ART amongst adolescents and young adults living with HIV. Future research and programming should seek to address psychosocial support interventions or approaches specifically designed to address the needs of adolescents and young adults living with HIV.PROSPERO: Registration CRD42018105057
Birth weight and renal markers in children aged 5 – 10 years in Cameroon: A cross-sectional study
Authors: Kaze Francois Folefack , Nguefack Seraphin , Asong Constantine Menkoh , Assob Jules Clement Nguedia , Nansseu Jobert Richie , Kowo Mathurin Pierre , Nzana Victorine , Kalla Ginette Claude Mireille , Halle Marie Patrice 
Published: August 2020

Abstract
Background A relationship exists between birth weight (BW) and glomerular filtration rate (GFR) in postnatal kidney. Willing to fill a gap of knowledge in sub-Saharan Africa, we assessed the effect of BW on blood pressure (BP), proteinuria and GFR among Cameroonians children.Methods This was a cross-sectional hospital-based study from January to April 2018 at the Yaounde Gynaeco-Obstetric and Paediatric Hospital (YGOPH). We recruited low BW (LBW) [<2500g], normal BW (NBW) [2500-3999g] and high BW (HBW) [>4000g] children, aged 5-10 years, born and followed-up at YGOPH. We collected socio-demographic, clinical (weight, height, BP), laboratory (proteinuria, creatinine), maternal and birth data. The estimated GFR was calculated using the Schwartz equation.Results We included 80 children (61.2% boys) with 21 (26.2%) LBW, 45 (56.2%) NBW and 14 (15.5%) HBW; the median (interquartile range) age was 7.3 (6.3-8.1) years and 17 (21.2%) were overweight/obese. Two (2.5%) children, all with a NBW (4.4%), had an elevated BP whereas 2 (2.5%) other children, all with a LBW (9.5%), had hypertension (p=0.233). Seven (8.7%) children had proteinuria with 19%, 2.2% and 14.3% having LBW, NBW and HBW, respectively (p=0.051). Equivalent figures were 18 (22.5%), 14.3%, 24.2% and 28.6% for decreased GFR, respectively (p=0.818). There was a trend towards an inverse relationship between BW and BP, proteinuria and GFR (p>0.05). Conclusion Proteinuria is more pronounced in childhood with a history of LBW and HBW while LBW children are more prone to develop hypertension. Regular follow-up is needed to implement early nephroprotective measures among children with abnormal BW.
TNFAIP8 variants as potential epidemiological and predictive biomarkers in ovarian cancer
Authors: Gao Hongyu , Zhang Zhiran , Jiang Liangliang , Zhang Lei , Qin Ling , Liu Tianbo , Yang Shanshan 
Published: August 2020

Abstract
Background: This research aimed to investigate the association between tumor necrosis factor-a-induced protein 8 (TNFAIP8) polymorphisms and ovarian cancer (OC) susceptibility. Methods: A case-control study of 210 patients with OC and 231 healthy controls was conducted to assess the association between TNFAIP8 polymorphisms (rs11064, rs1045241, and rs1045242) and OC risk in Heilongjiang Province of China. The SNaPshot SNP assay was conducted to detect SNP genotype. Logistic regression analysis was applied to illustrate the underlying association.Results: Our research found that TNFAIP8 rs11064 and rs1045242 were significantly connected with the susceptibility of OC. Additionally, rs1045242 increased the risk of OC, while rs11064 performed a protective role in the risk of OC. Data revealed that rs1045242 strongly related with advanced FIGO stage, larger residual tumor, and the presence of recurrence. Conclusions: TNFAIP8 genetic variants, which may play difference roles, were significantly associated with OC susceptibility. The underlying molecular mechanism needs be clarified with scientific evidence.
A Fourth-Order Cumulants Orthonormal Propagator Rooting Method Based on Toeplitz Approximation
Authors: Shi Heping , Ma Ning , Guan Zhiwei , Zhang Lizhu , Jiang Shan 
Published: August 2020

Abstract
A novel Toeplitz fourth-order cumulantsAorthonormal propagator rooting method of direction-of-arrivalnestimation for uniform linear arrayois proposed in this paper. Specifically, the modified (i.e., reduced-dimension)vmatrix is achieved at first via removing the redundant information encompassed in the primaryematrix, then the lmatrix which possesses Toepltiz structure can be recovered by utilizing the Toepltiz approximation method. To reduce the computational complexity, an effective method based on the polynomial rooting technology is adopted. Finally, the DOAs of incident signals can be estimated by exploiting orthonormal propagator rooting method. The theoretical analysis coupled with simulation results show that the proposed resultant algorithm can reduce the computational complexity signify- cantly, as well as improve the estimation performance in both spatially-white noise environment and spatially-color noise environment.
Establishment of Multifactor Predictive Models for the Occurrence and Progression of Cervical Intraepithelial Neoplasia
Authors: Chen Mengjie , Wang He , Liang Yuejuan , Hu Mingmiao , Li Li 
Published: August 2020

Abstract
Background: To study the risk factors involved in the occurrence and progression of cervical intraepithelial neoplasia (CIN) and to establish predictive models. Methods: Genemania was used to build a gene network. Then, the core gene-related pathways associated with the occurrence and progression of CIN were screened in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) experiments were performed to verify the differential expression of the identified genes in different tissues. R language was used for predictive model establishment. Results: A total of 10 genes were investigated in this study. A total of 30 cases of cervical squamous cell cancer (SCC), 52 cases of CIN and 38 cases of normal cervix were enrolled. Compared to CIN cases, the age of patients in the SCC group was older, the number of parities was greater, and the percentage of patients diagnosed with CINII+ by TCT was higher. The expression of TGFBR2, CSKN1A1, PRKCI and CTBP2 was significantly higher in the SCC groups. Compared to patients with normal cervix tissue, the percentage of patients who were HPV positive and were diagnosed with CINII+ by TCT was significantly higher. FOXO1 expression was significantly higher in CIN tissue, but TGFBR2 and CTBP2 expression was significantly lower in CIN tissue. The significantly different genes and clinical factors were included in the models. Conclusions: Combination of clinical and significant genes to establish the random forest models can provide references to predict the occurrence and progression of CIN.
A robust eleven-genes prognostic model can predict overall survival in bladder cancer patients based on five cohorts
Authors: Lin Jiaxing , Yang Jieping , Xu Xiao , Wang Yutao , Yu Meng , Zhu Yuyan 
Published: August 2020

Abstract
Background: Bladder cancer is the tenth most common cancer globally, but existing biomarkers and prognostic models are limited. Method: In this study, we used four bladder cancer cohorts from The Cancer Genome Atlas and Gene Expression Omnibus databases to perform univariate Cox regression analysis to identify common prognostic genes. We used the least absolute shrinkage and selection operator regression to construct a prognostic Cox model. Kaplan-Meier analysis, receiver operating characteristic curve, and univariate / multivariate Cox analysis were used to evaluate the prognostic model for the four cohorts. Finally, a co-expression network, CIBERSORT, and ESTIMATE algorithm were used to explore the mechanism related to the model.Results: A total of 11 genes were identified from the four cohorts to construct the prognostic model, including eight risk genes (SERPINE2, PRR11, DSEL, DNM1, COMP, ELOVL4, RTKN, and MAPK12) and three protective genes (FABP6, C16orf74, and TNK1). The 11-genes model could stratify the risk of patients in all five cohorts, and the prognosis was worse in the group with a high-risk score. The area under the curve values of the five cohorts in the first year are all greater than 0.65. Furthermore, this model's predictive ability is stronger than that of age, gender, grade, and T stage. Through the weighted co-expression network analysis, the gene module related to the model was found, and the key genes in this module were mainly enriched in the tumor microenvironment. B cell memory showed low infiltration in high-risk patients. Furthermore, in the case of low B cell memory infiltration and high-risk score, the prognosis of the patients was the worst.Conclusion: The proposed eleven-genes model is a promising biomarker for estimating overall survival in bladder cancer. This model can be used to stratify the risk of bladder cancer patients, which is beneficial to the realization of individualized treatment.
Synergistic effects of Lactobacillus plantarum and Staphylococcus carnosus on animal food components
Authors: Ivankin Andrey , Verevkin Alehey , Efremov Alexander , Vostrikova Natalia , Kulikovskii Andrey , Baburina Marina 
Published: August 2020

Introduction. Various cultures of microorganisms have recently been used to accelerate technological processes. In this regard, it appears highly relevant to study the action of beneficial microorganisms on the components of food systems.
Study objects and methods. The study objects included a model mixture of beef muscle and pork fat tissue with 2% salt, as well as a model protein. Lactobacillus plantarum and Staphylococcus carnosus were used in an amount of 1×107 CFU/g of raw material. The compositions of free amino and fatty acids, carbohydrates, and other components were analyzed by liquid and gas chromatography with mass-selective detection.
Results and discussion. We studied the effect of L. plantarum and S. carnosus on protein, lipid, and carbohydrate components of food systems based on animal raw materials. We found that the combined effect of the cultures was by 25% as effective as their individual use at 4×109 CFU/kg of raw material. The three-week hydrolysis of proteins to free amino acids was almost a third more effective than when the cultures were used separately. The synergistic effect of L. plantarum and S. carnosus on fat components was not detected reliably. Free monosaccharides formed more intensively when the cultures were used together. In particular, the amount of free lactose almost doubled, compared to the cultures’ individual action.
Conclusion. We described culture-caused quantitative changes in the main components of animal-based food systems: amino acids, fatty acids, carbohydrates, and basic organic compounds. Also, we identified substances that can affect the taste and aroma of final products when the cultures are used together or separately. These results make it possible to obtain products with a wide variety of sensory properties.
Scaling up the Enzymatic Hydrolysis of Bovine Plasma Protein to Produce an Antioxidant from a Biological Source
Authors: Grimaldos Nathalia A. Gómez , Zapata José E.M. 
Published: August 2020

Background:
modern society, there is a tendency to consume products with natural origins
and minimum chemical additives. This has encouraged the replacement of synthetic antioxidants
for the ones obtained from natural sources, such as the antioxidants acquired from enzymatic protein
hydrolysates.

Objective:
In this study, the process of enzymatic hydrolysis of proteins from bovine plasma, which
produces hydrolysates with an Antioxidant Capacity (AC), was scaled up from 1 to 5 L.

Methods:
An experimental design was developed in 1 L to evaluate the effect of the Substrate concentration
(So) on the time needed to reach a Degree of Hydrolysis (DH) of 20% as well as the AC.

Results:
The best conditions in the 1 L reactor controlled by a Titrando 842 were transferred to 5L in a
BioFlo310 reactor. These conditions were achieved at a ratio of 80g/L of the substrate and 0.89 AU of
Alcalase 2.4L/g of the substrate in order to obtain a level of 16.36 ± 0.21min of the 20% of DH and antioxidant
capacity of 58.98 ± 1.80%.

Conclusion:
The results showed that DH depends significantly on So, while the antioxidant capacity
only depends on the DH. Additionally, the dimensional analysis using Re as a scaling criterion allowed
us to obtain the same results in the model (1 L) and the prototype (5 L).
Prognostic Value of MiRNAs in Patients with Laryngeal Cancer: A Systematic Review and Meta-Analysis
Authors: Zou Wujun , Hu Xiaoyan , Wang Dingting , Jiang Liang 
Published: August 2020

Background:Many studies have explored the relationship between the expression level of miRNAs and the prognosis of patients with laryngeal cancer (LC). However, the prognostic value of miRNA in LC patients has not been comprehensively evaluated.Methods:PubMed, Web of Science, Embase, and Cochrane Database of Systematic Reviews were extensively searched for all studies published before the end of February 2020 that investigated the correlation between miRNA expression level and clinical prognosis in patients with LC.Results:Twenty-one studies involving 1784 patients were included in our meta-analysis. The survival endpoints of OS and DFS were 1.69 (95% CI 1.45-1.98; p < 0.05) and 3.62 (95% CI 2.34–5.62; p < 0.05), respectively. Both OS and DFS results were statistically significant. Subgroup analyses were performed by evaluating the effects of miR-196b, miR-375, and miR-21 on OS and the effects of miR-34c-5p on DFS. The results obtained for miR-196b and miR-34c-5p were statistically significant.Conclusion:The results indicate that miRNAs, as prognostic biomarkers for LC, play an important role in clinical value. In particular, miR-196b and miR-34c-5p have the potential to be used as prognostic biomarkers. However, further large-scale cohort studies based on these miRNAs are urgently needed to validate their clinical value and help determine the direction of future clinical work in the area.