bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-Cell Transcriptomic Profiling and Characterization of Endothelial Progenitor Cells: New Approach for Finding Novel Markers
Authors: Abdelgawad Mohamed Essameldin , Desterke Christophe , Uzan Georges , Naserian Sina 
Published: October 2020

Abstract
Background: Endothelial progenitor cells (EPCs) are promising candidates for the cellular therapy of peripheral arterial & cardiovascular diseases. However, hitherto there is no specific marker(s) defining precisely EPCs. Herein, we are proposing a new in-silico approach for finding novel EPCs markers. Methods: we assembled five groups of chosen EPCs-related genes/factors using Pubmed literature & gene ontology databases. This shortened database of EPCs factors was fed into publically-published transcriptome matrix to compare their expression between endothelial colony-forming cells (ECFCs), HUVECs and two adult endothelial cell types (ECs) from skin and adipose tissue. Further, the database was used for functional enrichment on mouse phenotype database and protein-protein interaction network analyses. Moreover, we built a digital matrix of healthy donors' PBMCs (33 thousand single cell transcriptomes) and analyzed the expression of these EPCs factorsResults: Transcriptome analyses showed that BMP2,4 & ephrinB2 were exclusively highly expressed in EPCs; the expression of neuropilin-1 & VEGF-C were significantly higher in EPCs & HUVECs compared with other ECs; Notch 1 was highly expressed in EPCs & skin-ECs; MIR21 was highly expressed in skin-ECs; PECAM-1 were significantly higher in EPCs & adipose ECs. Moreover, functional enrichment of EPCs-related genes on mouse phenotype and STRING protein database has revealed significant relations between chosen EPCs factors and endothelial & vascular functions, development and morphogenesis, where ephrinB2, BMP2 and BMP4 were highly expressed in EPCs and were connected to abnormal vascular functions. Single cell RNA-sequencing analyses has revealed that among the EPCs regulated markers in transcriptome analyses: i-ICAM1 & Endoglin were weekly expressed in the monocyte compartment of peripheral blood; ii-CD163 & CD36 were highly expressed in CD14+ monocyte compartment whereas CSF1R was highly expressed in CD16+ monocyte compartment; iii-L-selectin & IL6R were globally expressed in the lymphoid/myeloid compartments; iv-interestingly, PLAUR/UPAR & NOTCH2 were highly expressed in both CD14+ & CD16+ monocytic compartments. Conclusions: The current study has identified novel EPCs markers that could be used for better characterization of EPCs sub-population in adult peripheral blood and subsequent usage of EPCs for various cell therapy and regenerative medicine applications.
Enhanced Renoprotective Effect of GDNF-Modified Adipose Derived Mesenchymal Stem Cells on Renal Interstitial Fibrosis
Authors: Li Shulin , Wang Yanping , Wang Zhuojun , Chen Lu , Zuo Bangjie , Liu Caixia , Sun Dong 
Published: October 2020

Abstract
Background: The therapeutic effect of Mesenchymal stem cells (MSCs) from human adipose tissue on renal interstitial fibrosis has been demonstrated by several groups. However, the way to enhance the renoprotective effect of Adipose Derived Mesenchymal Stem Cells (AMSCs) and the possible mechanisms, are still unclear. The present study aimed to determine whether glial cell line-derived neurotrophic factor (GDNF)-modified AMSCs holds an enhanced protective effect on renal fibrosis. Methods: AMSCs were isolated and purified for culture. GDNF gene was constructed to transfect into AMSCs. The ability of AMSCs and GDNF-AMSCs supernatants to promote tube formation of endothelial cells, repair damaged endothelial cell junctions and improve endothelial cell function was compared by tube formation assay, immunofluorescence techniques and vascular ring assay, respectively. Furthermore, HE and Masson staining were used to observe the histological morphology of the kidney in vivo. Peritubular Capillary Changes were detected and analysed by Fluorescence Micro-angiography (FMA). Meanwhile, the endothelial cell, hypoxia, oxidative stress, fibrotic markers and PI3K/Akt pathway proteins were measured by western blot or qRT-PCR technics. Results: Studies in vitro demonstrated that compared with AMSCs only, GDNF-AMSCs could better repair injured endothelial cells and promote angiogenesis through secreting more growth factors in the supernatant of GDNF-AMSCs culture media. Studies in vivo, unilateral ureteral obstruction (UUO)-induced fibrotic mice were injected with transfected AMSCs through their tail veins. We showed that enhanced homing of AMSCs were observed in the GDNF-AMSC group compared with the AMSC group. GDNF-AMSCs treated animals exhibited better improvement of capillary rarefaction and fibrosis induced by obstructed kidney compared with the AMSC group. Furthermore, we reported that GDNF-AMSCs protect renal tissues against microvascular injuries via activation of the PI3K/Akt signaling pathway. Therefore, GDNF-AMSCs further ameliorated the tissue hypoxia, suppressed oxidative stress, and finally inhibited endothelial to mesenchymal transition noting by decreased coexpression of endothelial cell (CD31) and myofibroblast (a-SMA) markers. Conclusion: Collectively, our data indicated that the GDNF gene enhances the ability of AMSCs in improving renal microcirculation through PI3K/Akt/eNOS signaling pathway, and afterward inhibit the EndMT process and kidney fibrogenesis, which should have wide implications in designing future remedies for chronic kidney disease (CKD) treatment.
A comment on “An evolutionary system of mineralogy: Proposal for a classification of planetary materials based on natural kind clustering”
Authors: Hatert Frédéric , Mills Stuart J. , Hawthorne Frank C. , Rumsey Mike S. 
Published: October 2020

Abstract
The classification and nomenclature of mineral species is regulated by the Commission on New Minerals, Nomenclature and Classification of the International Mineralogical Association (IMACNMNC). This mineral species classification is necessary for Earth Sciences, as minerals constitute most planetary and interstellar materials. Hazen (2019) has proposed a classification of minerals and other Earth and planetary materials according to “natural clustering.” Although this classification is complementary to the IMA-CNMNC mineral classification and is described as such, there are some unjustified criticisms and factual errors in the comparison of the two schemes. It is the intent of the present comment to (1) clarify the use of classification schemes for Earth and planetary materials, and (2) counter erroneous criticisms or statements about the current IMA-CNMNC system of approving proposals for new mineral species and classifications.
Temporal analysis of water, sanitation, and hygiene data from knowledge, attitudes, and practices surveys in the protracted humanitarian crisis in Myanmar
Authors: Domini Marta , Guidotti Sunny , Lantagne Daniele 
Published: October 2020

Abstract
Knowledge, attitudes, and practices (KAP) surveys are commonly conducted by humanitarian actors at project baseline and endline. We hypothesized summarized KAP data could inform water, sanitation, and hygiene (WASH) programming and strategies. In conflict-affected Myanmar, the WASH Cluster collated KAP documents from 2011 to 2016. After developing a methodology for KAP temporal analysis, we reviewed provided documents against inclusion criteria; extracted and categorized data; created a WASH activities and emergency events timeline; completed temporal analysis; and triangulated and summarized results. Sixteen documents met inclusion criteria assessing WASH data at the national level (19%), in Rakhine (56%) or Kachin State (25%). Twenty-five WASH metrics were extracted and categorized. We found WASH knowledge and attitudes (e.g. handwashing knowledge) increased over time; practices (e.g. handwashing) also increased, but at a lower rate and less consistently; and increases were related to WASH programming. Using this methodology, we identified gaps (safe water storage, risk of children's feces, focus on vulnerable populations), barriers (cultural and religious beliefs), and programmatic recommendations (continue hygiene promotion, focusing on closing gaps, and improve waste collection systems) in Myanmar. Additionally, to enable temporal analysis and improve WASH programming, we recommend WASH Clusters standardize KAP data collection tools and analysis in the future.
Focus on MicroRNAs as Biomarker in Pediatric Diseases
Authors: Lassandro Giuseppe , Ciaccia Loredana , Amoruso Anna , Palladino Valentina , Palmieri Viviana V. , Giordano Paola 
Published: October 2020

Background:
MiRNAs are a class of small non-coding RNAs that are involved in the post-transcriptional
regulation of gene expression. MiRNAs are considered a class of epigenetic biomarkers. These biomarkers
can investigate disease at different stages: diagnosis, therapy or clinical follow-up.

Objective:
The aim of this paper is to highlight the innovative use of miRNAs in several childhood diseases.

Methods:
We conducted a literature review to search the usage of miRNAs in pediatric clinical routine or experimental
trials.

Results:
We found a possible key role of miRNAs in different pediatric illnesses (metabolic alterations, coagulation
defects, cancer).

Conclusion:
The modest literature production denotes that further investigation is needed to assess and validate
the promising role of miRNAs as non-invasive biomarkers in pediatric disorders.
Inter-relações no cuidado com as plantas medicinais – “vem de berço”
Authors: Almeida Camila , Vaz Ribeiro Márcia , Kaster Portelinha Márcia , Griebeler Oliveira Stefanie , Barbieri Rosa Lía 
Published: October 2020

O trabalho teve como objetivo descrever as inter-relações de pessoas que realizam o cuidado em saúde com plantas medicinais. Trata-se de uma pesquisa qualitativa, exploratória, descritiva e etnobotânica que utilizou a Teoria Sistêmica como referencial teórico. Foram entrevistadas seis pessoas referência no cuidado em saúde com plantas medicinais no primeiro semestre de 2018. Os dados foram organizados pelo programa WebQDA qualitative data analysis e foi realizada a análise de conteúdo de Bardin. São apresentados resultados relacionados a origem do saber sobre o cuidado com plantas medicinais e também acerca da inter-relação social como forma de transmissão de conhecimento. O saber é adquirido na família, por cursos ou, é entendido como um dom. Os informantes transmitem seus saberes em diferentes contextos sociais conforme a disposição de suas inter-relações. Conclui-se que diferentes inter-relações individual, social e ambiental originam diferentes formas de cuidado em saúde com plantas medicinais e seus saberes associados.
DAS GREVES DE 1978 À BOLSONARIZAÇÃO DO BRASIL
Authors: Leal Pereira Neto Murilo 
Published: October 2020

A fim de compreender a “bolsonarização do Brasil”, o presente artigo analisa três momentos históricos: as greves metalúrgicas de 1978-1980, a formação do Partido dos Trabalhadores, ligada a essas lutas e a sua atuação nas eleições de 1982, e a derrota eleitoral de Fernando Haddad nas eleições municipais de 2016. O fio condutor é a formação e o enfraquecimento de uma coalizão operária e popular como sujeito democrático. Conclui-se que sua reconstrução é condição para revigoramento da democracia.[1] Como método, o artigo adota a orientação progressiva/regressiva de Henri Lefebvre: partir do problema percebido no presente, estabelecer seu estatuto teórico, investigar sua gênese em um ponto determinado do passado, reconstruir sua historicidade e retornar ao presente compreendendo-o melhor.
Palavras-chave: Greves metalúrgicas. Democracia. Partido dos Trabalhadores. Eleições.

[1] Agradeço a José Francisco de Oliveira Mattos pela colaboração técnica e intelectual na revisão deste artigo.
MEMÓRIAS CRUZADAS DE MOVIMENTOS ESPECÍFICOS
Authors: Leite Lopes José Sergio , Matos Pereira José Carlos , Carriço Antonio , Starosky Míriam 
Published: October 2020

O artigo propõe uma comparação entre práticas de lutas por direitos exercidas por trabalhadores urbanos e trabalhadores rurais através do estudo dos ciclos de greves que ocorreram entre metalúrgicos da área metropolitana de São Paulo e canavieiros de Pernambuco, nos dois últimos anos da década de 70 e nos anos 80 do século passado. O texto centra mais especificamente sua análise no processo de acumulação de forças durante a ditadura, no período anterior aos ciclos de greves.

Palavras-chave: Práticas sindicais. Metalúrgicos. Canavieiros. Ciclos de greves.
A POTÊNCIA DOS FEMINISMOS NA LUTA CONTRA A RAZÃO NEOLIBERAL NA AMÉRICA LATINA
Authors: Vilaça Mônica , Freitas Bárbara 
Published: October 2020

Esta entrevista com Verónica Gago, professora da Universidade de Buenos Aires (UBA) e da Universidade Nacional de San Martin (UNSAM), busca dialogar com as recentes movimentações construídas no bojo das lutas e interpretações que têm marcado os últimos anos na América Latina. Este tem sido um período atravessado por uma pujante produção e tradução para o português de livros de mulheres intelectuais e feministas de várias tradições, e por intensas mobilizações pelos direitos das mulheres, como as lutas pelo direito ao aborto e de enfrentamento ao feminicídio, que criaram ressonância em diversos países e continentes, e que vêm provocando uma inflexão por novas perguntas e métodos de ler, interpretar e incidir na realidade social. No conjunto destas articulações, tem-se ampliado ações que buscam melhor conhecer a produção latino-americana e é neste movimento que se inserem os diálogos com Verónica Gago. Em nosso encontro, durante o Seminário Internacional “As perspectivas feministas sobre a geopolítica global patriarcal e racista”, realizado em Salvador (BA), em 2019[1] – momento de articulação dos movimentos e intelectuais feministas da América Latina, que coincidia com a visita realizada pela italiana Silvia Federici para o lançamento do livro “O Ponto Zero da Revolução: trabalho doméstico, reprodução e luta feminista” –, propusemos a realização desta entrevista. Com a impossibilidade de realizá-la durante o seminário, contamos com a generosidade de Verónica de responder às perguntas que formulamos por escrito. Esta forma de realização da entrevista permitiu uma elaboração densa e rica, e que compartilha também novos debates produzidos pós-seminário, que coincidem com novas publicações suas, mencionadas ao longo da entrevista. Nossa tradução buscou preservar com o máximo cuidado as complexas elaborações apresentadas, mantendo aspectos da formatação do texto da entrevista – grifos e itálicos – enviado por Verónica, que buscavam destacar e salientar algumas ideias nas análises. Esperamos que o diálogo consolidado nesta entrevista contribua com a partilha, mas também o reconhecimento, das elaborações que se têm construído na íntima relação entre academia e militância, expressas na experiência da Verónica Gago e que refletem uma estratégia de produção de conhecimento mobilizada por muitas mulheres nas universidades da América Latina.

[1] Evento realizado a partir da articulação entre o Coletivo de Mulheres Calafate/AMB Bahia, a Editora Elefante, o Instituto Eqüit e Rebrip, o Instituto Odara e a Rede de Mulheres Negras da Bahia, o PPGNeim/UFBA, a Réd de Gérnero y Comérico, o Fondo de Mujeres del Sur e Heinrich Böll Stiftung Brasil.
OS TRABALHADORES CANAVIEIROS DE PERNAMBUCO E O “NOVO SINDICALISMO”
Authors: Guedes Ladosky Mario Henrique , Oliveira Roberto Véras de 
Published: October 2020

As greves ocorridas entre 1978 e os primeiros anos da década de 1980 projetaram o Sindicato dos Metalúrgicos de São Bernardo do Campo e Diadema (SP) e a figura de Luís Inácio Lula da Silva como expressões de um novo campo de referência no sindicalismo brasileiro, conhecido como “novo sindicalismo”, tendo sido essa a matriz para a criação do Partido dos Trabalhadores (PT) e da Central Única dos Trabalhadores (CUT). No mesmo período, no Nordeste do país, na tradicional plantation de cana-de-açúcar, os trabalhadores vinham rearticulando uma história de lutas que havia tido importante expressão no período pré-64 e que não tinha sido completamente interrompida durante o regime militar. A greve dos canavieiros de Pernambuco, em 1979, foi um marco na retomada da luta sindical no campo. Entretanto o ciclo de greves de canavieiros no Nordeste, que se estendeu até meados dos 1980, não alcançou a mesma repercussão política e relevância acadêmica que as greves do ABC e o “novo sindicalismo”, sobre os quais os estudos de John Humphrey, Amnéris Maroni, Ricardo Antunes, Laís Abramo, Iram Jácome Rodrigues, entre outros, debateram acerca de seus desdobramentos políticos. Este artigo retoma a memória das greves dos canavieiros de Pernambuco e as práticas sindicais que a elas estiveram associadas, procurando discutir seus significados para a trajetória do sindicalismo brasileiro desde então. Busca-se revisitar as interpretações produzidas sobre aqueles acontecimentos, de modo a levantar algumas problematizações, sobretudo em relação ao tema do “novo sindicalismo”.
Palavras-chave: Greves. Canavieiros. Pernambuco. Sindicalismo.
Garanticemos la calidad de las muestras respiratorias para diagnosticar COVID-19
Authors: Gómez-Garzón Marcela , Isaza-Cortes Martha Patricia , Ibañez-Galvis Catalina 
Published: October 2020

Este artículo y video disponibles en el OJS de la Revista describe los procedimientos para la recolección de hisopos respiratorios que garantizan la bioseguridad y el diagnóstico de COVID-19 en adultos y niños.
Linking pre- and post-adoption records for research in anonymised form in a data safe haven:
Authors: Jones Kerina , Heys Sharon , Thompson Rachel , Griffiths Lucy , Johnson Rhodri , Lee Alex , Ford David , Broadhurst Karen 
Published: October 2020

Introduction: The long-term health and wellbeing of adoptees is under-researched. One reason for this has been limited data accessibility regarding the adoption process, and another is a practice common in some UK jurisdictions of changing the NHS number (or equivalent) at adoption, as part of creating the new identity. The SAIL Databank holds data from the Wales Children and Family Court Advisory and Support Service (Cafcass Cymru), together with children’s social care data, and can link these with routine health and administrative data in anonymised form. However, because the linkage key at SAIL is based on an encryption of the NHS number, working with pre- and post-adoption records for longitudinal research remains a major challenge. We set out to explore the legal implications of, and social support for, linking these records for use in anonymised form for longitudinal research.
Methods: We reviewed the main legislation and regulations governing the use of data about adoptees in England and Wales. We gauged support for a social licence in Wales by carrying out interviews with individuals who had been involved in the adoptions process, and by engaging with general public groups for their views. We drew out the main emerging themes and, in combination with the review, propose a way forward.
Results: The legal review indicated that there are provisions in the Family Procedure Rules (England and Wales) and the General Data Protection Regulation that can be relied upon for the lawful processing of adoption data into anonymised form for research. The main points of concern about linking pre- and post-adoption records were privacy, data security, the need to limit the number of organisations involved in data sharing, and re-identification risk. The over-riding message was favourable with longitudinal research seen as strongly beneficial.
Conclusion: This study has indicated that in Wales, there is no legal impediment, nor major objection from individuals involved in the adoptions process, as well as the general public for the use of adoption data in anonymised form, in a data safe haven. This includes the linkage of pre- and post‑adoption records to enable novel longitudinal research to take place. The provisos were that robust safeguards must be in place, and that the research should aim to benefit adoptees and to improve policy and practice. We conclude that it is reasonable to proceed with caution to develop practical ways to link pre- and post‑adoption records in a data safe haven.
Morphofunctional Characteristic of Edema-Swelling of the Cerebral Cortex of White Rats After Severe Traumatic Brain Injury Without the Use of L-Lysine Escinate and Against the Background of Its Use
Authors: Koshman I. P. , Stepanov S. S. , Shoronova A. Y. , Kalinichev A. G. , Akulinin V. A. , Avdeyev D. B. , Sergeyev V. I. 
Published: October 2020

Aim of study The study is devoted to a morphometric assessment of the manifestations of edema-swelling of the somatosensory cortex (SSC) of the brain of white rats after severe traumatic brain injury (TBI) without using L-lysine escinate and when using it as a therapeutic effect.Material and methods We stained sections with hematoxylin-eosin and performed morphometric methods. On thin (4 μm) serial frontal sections of SSC, neurons and microvessels in the control (intact animals, n=5) were examined in 1 (n= 5), 3 (n=5), 5 (n=5), 7 (n=5) and 14 (n=5) days after injury without treatment (n=25, comparison group) and with treatment (n=25, main group). In color raster images (lens x100), using the plug-in filter “Find Maxima”, maximum brightness areas (MBA) were determined , which were then analyzed using the “Analyze Particles” program from ImageJ 1.52 s. MBA corresponded to SSC sites with a high degree of hydration of nerve tissue - edema-swelling. Statistical hypotheses were tested using nonparametric criteria.Results and discussion In control animals, a low degree of hydration of SSC tissue was noted (relative area 3-8%). In the comparison group, 1 and 3 days after STBI, foci of edema-swelling covered up to 30% of SSC, in 5 days - up to 15%, in 7 days - up to 20%, in 15 - up to 18%. Significant heteromorphism and heterogeneity of changes in the neuropil around neurons and blood vessels was noted. In the dynamics of the post-traumatic period, the proportion of large foci of edema-swelling (intra- and perineuronal, perivascular) decreased. In the main group, one day after STBI, there was a statistically significantly smaller number of foci of edema-swelling and their total relative area. The values range of these variables significantly decreased. L-lysine escinat affected the water balance most effectively in the acute post-traumatic period (day 1 and 3). The drug “smoothed out” the manifestation peaks (number, focal area) of edema-swelling: the values of the studied morphometric indicators were statistically significantly different. Consequently, morphometric signs of hydropic dystrophy after STBI were detected in both studied groups during the 15 days of observation.Conclusion The degree of SSC nervous tissue hydration increased after STBI. L-lysine escinate statistically significantly reduced manifestations of hydropic dystrophy. The drug significantly affected the degree of hydration of neural tissue observed in the early post-traumatic period.