bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adaptação cultural em Português Brasileiro da Derriford Appearance Scale – 24 (DAS-24) para pessoas vivendo com HIV/AIDS
Authors: Martins Marcos Alberto , Neves Angela Nogueira , Moss Timothy , Martins Walter Henrique , Pereira Gerson Vilhena , Pessôa Karina Viviani de Oliveira , Silva Mariliza Henrique da , Abreu Luiz Carlos de 
Published: November 2019

Introduction: Body image can be defined as the representation of beliefs, emotions and perceptions about the body itself, manifested in behaviors directed to the body. When the body changes because of a disease and does not seem healthy, the self-concept may be severely challenged. People living with HIV/AIDS (PLHA) are particularly vulnerable to the distress and psychosocial impact of appearance, but in Brazil the assessment of those body image changes was subjective because there was not an available scale in Brazilian Portuguese to assess body image changes in clinical practice or research. Objective: To carry out the cross-cultural adaptation to the Brazilian Portuguese of the Derriford Appearance Scale 24 (DAS-24), with the verification of the linguistic, semantic, conceptual and cultural equivalence of the people living with HIV/AIDS in Brazil. Methods: We followed the five stages of culturally sensitive translation: direct translations, synthesis of translations, back-translations, expert committee meeting and pre-tests. The process of cultural adaptation was presented in a descriptive and analytical way, following patterns of methodological studies. The minimum, maximum and median values of the responses of each item were calculated from the pool of data from the third pretest group of 50 participants. The median of the item scores, the correlation on each item with the total score and the internal reliability, were calculated using the Cronbach alpha test. Results: The analysis of the responses of the last pre-test group indicated that attention must be given to itemsA, H, T and V in a future psychometric study. The present study is not enough for this scale to be used in clinical practice. To ensure that the culturally adapted instrument generates valid and reliable data, a subsequent study investigating its psychometric properties should be conducted. Conclusion: The cross-cultural adaptation of the Derriford Appearance Scale 24 (DAS-24) in its components of linguistic, semantic, conceptual and cultural equivalence to Brazilian Portuguese for the population of people living with HIV/AIDS was fully carried out. Despite this achievement, it is emphasized that the use of the Brazilian versionof DAS-24 in research and clinical routine is advised only after a psychometric study with this instrument.
Prevalência e fatores associados à obesidade em crianças menores que cinco anos no município de Rio Branco – Acre
Authors: Silva Delcio Damasceno da , Lima Marcos Venicius Malveira de , Muniz Pascoal Torres , Holanda Marlon Negreiros de , Câmara Ozianndeny Ferreira , Monteiro Adilson , Wajnsztejn Rubens 
Published: November 2019

Introduction: The nutritional status of children is considered an important instrument in measuring the health conditions and quality of life of a population. The increasing prevalence of obesity in children is a significant public health problem as it is an important risk factor for obesity in adulthood. Objective: To evaluate the prevalence and factors associated with childhood obesity in the city of Rio Branco - Acre. Method: A database analysis was carried out from the Risk Factors and Morbidity Survey for Noncommunicable Diseases in the Municipality of Rio Branco / Health and Nutrition for Adults and Children in 2008.This cross-sectional study examined 687 children aged 0 to 60 months in urban and rural areas. Statistical analysis considered expansion and sample design. The prevalence of childhood obesity according to the anthropometric indices P / I (weight for age), P / E (weight for height) and BMI / I (Body Mass Index) were respectively 6.85% (95% CI = 5.30). - 8.80), 6.66% (95% CI = 3.22 - 13.27) and 6.61% (95% CI = 3.25 - 12.98). Results: The final model showed a higher prevalence of obesity in the BMI / I index under the following conditions: home in the urban area (PR = 6.81; 95% CI = 1.27 - 36.38), electric lighting without meter (PR = 2.10; 95% CI = 1.22 - 3.59), mother’s height greater than 163cm (PR = 2.24; 95% CI = 1.12 - 4.47) and maternal obesity (RP = 2.37 95% CI = 1.19 - 4.72). Conclusion: The prevalence of obesity in the BMI / I index was high and is related to socioeconomic factors and specific maternal characteristics. It is necessary to promote actions that lead to the formation of a healthy lifestyle even in childhood.
The Nbp35/ApbC homolog acts as a nonessential [4Fe‐4S] transfer protein in methanogenic archaea
Authors: Zhao Cuiping , Lyu Zhe , Long Feng , Akinyemi Taiwo , Manakongtreecheep Kasidet , Söll Dieter , Whitman William B. , Vinyard David J. , Liu Yuchen 
Published: November 2019

The nucleotide binding protein 35 (Nbp35)/cytosolic Fe‐S cluster deficient 1 (Cfd1)/alternative pyrimidine biosynthetic protein C (ApbC) protein homologs have been identified in all three domains of life. In eukaryotes, the Nbp35/Cfd1 heterocomplex is an essential Fe‐S cluster assembly scaffold required for the maturation of Fe‐S proteins in the cytosol and nucleus, whereas the bacterial ApbC is an Fe‐S cluster transfer protein only involved in the maturation of a specific target protein. Here, we show that the Nbp35/ApbC homolog MMP0704 purified from its native archaeal host Methanococcus maripaludis contains a [4Fe‐4S] cluster that can be transferred to a [4Fe‐4S] apoprotein. Deletion of mmp0704 from M. maripaludis does not cause growth deficiency under our tested conditions. Our data indicate that Nbp35/ApbC is a nonessential [4Fe‐4S] cluster transfer protein in methanogenic archaea.
SCN1B‐linked early infantile developmental and epileptic encephalopathy
Authors: Aeby Alec , Sculier Claudine , Bouza Alexandra A. , Askar Brandon , Lederer Damien , Schoonjans Anne‐Sofie , Vander Ghinst Marc , Ceulemans Berten , Offord James , Lopez‐Santiago Luis F. , Isom Lori L. 
Published: November 2019

AbstractObjectivePatients with Early Infantile Epileptic Encephalopathy (EIEE) 52 have inherited, homozygous variants in the gene SCN1B, encoding the voltage‐gated sodium channel (VGSC) β1 and β1B non‐pore‐forming subunits.MethodsHere, we describe the detailed electroclinical features of a biallelic SCN1B patient with a previously unreported variant, p.Arg85Cys.ResultsThe female proband showed hypotonia from birth, multifocal myoclonus at 2.5 months, then focal seizures and myoclonic status epilepticus (SE) at 3 months, triggered by fever. Auditory brainstem response (ABR) showed bilateral hearing loss. Epilepsy was refractory and the patient had virtually no development. Administration of fenfluramine resulted in a significant reduction in seizure frequency and resolution of SE episodes that persisted after a 2‐year follow‐up. The patient phenotype is more compatible with early infantile developmental and epileptic encephalopathy (DEE) than with typical Dravet syndrome (DS), as previously diagnosed for other patients with homozygous SCN1B variants. Biochemical and electrophysiological analyses of the SCN1B variant expressed in heterologous cells showed cell surface expression of the mutant β1 subunit, similar to wild‐type (WT), but with loss of normal β1‐mediated modification of human Nav1.1‐generated sodium current, suggesting that SCN1B‐p.Arg85Cys is a loss‐of‐function (LOF) variant.InterpretationImportantly, a review of the literature in light of our results suggests that the term, early infantile developmental and epileptic encephalopathy, is more appropriate than either EIEE or DS to describe biallelic SCN1B patients.
2019 Update of the American College of Rheumatology Recommended Rheumatoid Arthritis Disease Activity Measures
Authors: England Bryant R. , Tiong Benedict K. , Bergman Martin J. , Curtis Jeffrey R. , Kazi Salahuddin , Mikuls Ted R. , O'Dell James R. , Ranganath Veena K. , Limanni Alex , Suter Lisa G. , Michaud Kaleb 
Published: November 2019

ObjectiveTo provide updated American College of Rheumatology (ACR) recommendations on rheumatoid arthritis (RA) disease activity measurements to facilitate a treat‐to‐target approach in routine clinical care.MethodsA working group conducted a systematic literature review from the time of the prior ACR recommendations literature search. Properties of disease activity measures were abstracted, and study quality was assessed using the Consensus‐Based Standards for the selection of Health Measurement Instruments 4‐point scoring method, allowing for overall level of evidence assessment. Measures that fulfilled a minimum standard were identified, and through a modified Delphi process preferred measures were selected for regular use in most clinic settings.ResultsThe search identified 5,199 articles, of which 110 were included in the review. This search identified 46 RA disease activity measures that contained patient, provider, laboratory, and/or imaging data. Descriptions of the measures, properties, study quality, level of evidence, and feasibility were abstracted and scored. Following a modified Delphi process, 11 measures fulfilled a minimum standard for regular use in most clinic settings, and 5 measures were recommended: the Disease Activity Score in 28 Joints with Erythrocyte Sedimentation Rate or C‐Reactive Protein Level, Clinical Disease Activity Index, Simplified Disease Activity Index, Routine Assessment of Patient Index Data 3, and Patient Activity Scale‐II.ConclusionWe have updated prior ACR recommendations for preferred RA disease activity measures, identifying 11 measures that met a minimum standard for regular use and 5 measures that were preferred for regular use in most clinic settings.
Responses to Cytokine Inhibitors Associated with Cellular Composition in Models of Immune‐Mediated Inflammatory Arthritis
Authors: Nielsen Morten A. , Lomholt Søren , Mellemkjær Anders , Andersen Morten N. , Buckley Christopher D. , Kragstrup Tue W. 
Published: November 2019

ObjectiveImmune‐mediated inflammatory arthritis (IMIA) is a heterogeneous group of diseases including rheumatoid arthritis (RA), psoriatic arthritis (PsA), and spondyloarthritis (SpA). Disease‐modifying antirheumatic drugs (DMARDs) target very different cellular components of the disease processes. Characterization of the pathobiological subtypes of IMIA could provide more specific treatment approaches for each disease. For example, RA has been proposed to consist of at least three synovial pathotypes (lymphoid, myeloid, and fibroid), and only a subgroup of RA patients have erosive disease. The objective of this study was to evaluate the effects of various DMARDs on different synovial cell subsets using human ex vivo models of IMIA.MethodsSynovial fluid and blood samples were obtained from a study population consisting of patients with RA, PsA, or peripheral SpA with at least one swollen joint (n = 18). The DMARDs used in this study were methotrexate, adalimumab, etanercept, tocilizumab, anakinra, ustekinumab, secukinumab, tofacitinib, and baricitinib. Paired synovial fluid mononuclear cells (SFMCs), peripheral blood mononuclear cells (PBMCs), and fibroblast‐like synovial cells (FLSs) were used in three different previously optimized ex vivo models.ResultsIn SFMCs cultured for 48 hours, all DMARDs except anakinra decreased the production of monocyte chemoattractant protein (MCP)‐1. In SFMCs cultured for 21 days, only the two tumor necrosis factor alpha (TNFα) inhibitors adalimumab and etanercept decreased the secretion of tartrate‐resistant acid phosphatase (P < 0.01, P < 0.001). In the FLS and PBMC 48‐hour co‐cultures, only tocilizumab (P < 0.001) and the two Janus kinase inhibitors tofacitinib and baricitinib (both P < 0.05) decreased the production of MCP‐1 by around 50%.ConclusionTNFα inhibition was effective in preventing inflammatory osteoclastogenesis, whereas tocilizumab, tofacitinib, and baricitinib had superior efficacy in cultures dominated by FLSs. Taken together, this study reveals that responses to cytokine inhibitors associate with cellular composition in models of IMIA. In particular, this study provides new evidence on the differential effect of DMARDs on leukocytes compared with stromal cells.
Internalized and Anticipated Stigmatization in Patients With Gout
Authors: Kleinstäuber Maria , Wolf Leonie , Jones Annie S. K. , Dalbeth Nicola , Petrie Keith J. 
Published: November 2019

ObjectiveTo investigate the relationship between stigma perception and demographic, clinical, and psychosocial variables.MethodsA sample of 50 patients with gout and prescribed urate‐lowering medication (84% were males, mean serum urate 0.34 mmol/l) completed questionnaires on internalized and anticipated stigma, demographics, clinical gout‐related variables, and psychosocial variables (illness perceptions, illness‐related disability, illness‐related body satisfaction, intentional nonadherence). Serum urate level was obtained from the most recent blood test.ResultsIn this sample, 26% experienced internalized stigma, 26% expected to be stigmatized by friends or family members, and 14% by health care workers. Univariate regression analyses showed that younger age, ethnicity other than New Zealand European, increased severity of gout pain, cognitive and emotional illness perceptions, greater illness‐related disability, and increased intentional nonadherence to urate‐lowering medication were associated with increased internalized and anticipated stigma. Younger age, emotional illness response, and intentional nonadherence were the only variables explaining incremental variance of the experience of anticipated stigma in a multivariate regression model.ConclusionInternalized and anticipated illness‐related stigma was reported by a subgroup of patients with gout. The experience of stigma is associated with younger age, a negative emotional illness response, and intentions to not adhere with a medical treatment.
Rapid Biofabrication of Printable Dense Collagen Bioinks of Tunable Properties
Authors: Griffanti Gabriele , Rezabeigi Ehsan , Li Jingjing , Murshed Monzur , Nazhat Showan N. 
Published: November 2019

AbstractRecent convergence of the 3D printing of tissue‐like bioinks and regenerative medicine offers promise in the high‐throughput engineering of in vitro tissue models and organoids for drug screening and discovery research, and of potentially implantable neo‐tissues with tailored structural, biological, and mechanical properties. However, the current printing approaches are not compatible with collagen, the native scaffolding material. Herein, a unique biofabrication approach that uses automated gel aspiration‐ejection (GAE) is reported to potentially overcome these challenges. Automated‐GAE generates highly defined, aligned, dense collagen gel bioinks of various geometries (i.e., cylindrical, quadrangular, and tubular), dimensions, as well as tunable microstructural and mechanical properties that modulate seeded cellular responses. By densifying initial naturally derived reconstituted collagen hydrogels incorporating cells, automated‐GAE generates mini‐tissue building blocks with tailored protein fibril density and alignment, as well as cell loading, density and orientation according to the intended use. Surprisingly, a simple mathematical relationship defining the bioink compaction factor is found to be highly effective in predicting the initial and temporal properties of the bioinks in culture. Therefore, automated‐GAE will potentially also enable a fourth dimension to biofabrication, where cell–cell communications and cell‐extracellular matrix interactions as a function of time in culture can be predicted and modeled.
Heme Cofactor‐Resembling Fe–N Single Site Embedded Graphene as Nanozymes to Selectively Detect H2O2 with High Sensitivity
Authors: Kim Min Su , Lee Junsang , Kim Hye Su , Cho Ara , Shim Kyu Hwan , Le Thao Nguyen , An Seong Soo A. , Han Jeong Woo , Kim Moon Il , Lee Jinwoo 
Published: November 2019

AbstractOver the past decade, the catalytic activity of nanozymes has been greatly enhanced, but their selectivity is still low and considered a critical issue to overcome. Herein, Fe–N4 single site embedded graphene (Fe–N‐rGO), which resembles the heme cofactor present in natural horseradish peroxidase, shows a marked enhancement in peroxidase‐like catalytic efficiency of up to ≈700‐fold higher than that of undoped rGO as well as excellent selectivity toward target H2O2 without any oxidizing activity. Importantly, when Fe or N is doped alone or when Fe is replaced with another transition metal in the Fe–N4 site, the activity is negligibly enhanced, showing that mimicking the essential cofactor structure of natural enzyme might be essential to design the catalytic features of nanozymes. Density functional theory results for the change in Gibbs free energy during the peroxide decomposition reaction explain the high catalytic activity of Fe–N‐rGO. Based on the high and selective peroxidase‐like activity of Fe–N‐rGO, trace amounts of H2O2 produced from the enzymatic reactions from acetylcholine and cancerous cells are successfully quantified with high sensitivity and selectivity. This work is expected to encourage studies on the rational design of nanozymes pursuing the active site structure of natural enzymes.
Biomedical Applications of DNA‐Based Hydrogels
Authors: Gačanin Jasmina , Synatschke Christopher V. , Weil Tanja 
Published: November 2019

AbstractNucleic acids are gaining significant attention as versatile building blocks for the next generation of soft materials. Due to significant advances in the chemical synthesis and biotechnological production, DNA becomes more widely available enabling its usage as bulk material in various applications. This has prompted researchers to actively explore the unique features offered by DNA‐containing materials like hydrogels. In this review article, recent developments in the field of hydrogels that feature DNA as a component either in the construction of the material or as functional unit within the construct and their biomedical applications are discussed in detail. First, different synthetic approaches for obtaining DNA hydrogels are summarized, which allows classification of DNA materials according to their structure. Then, new concepts, properties, and applications are highlighted such as DNA‐based biosensor devices, drug delivery platforms, and cell scaffolds. With the 2018 Nobel Prize in Physiology or Medicine being awarded to cancer immunotherapy underscoring the importance of this therapy, DNA hydrogel systems designed to modulate the immune system are introduced. This review aims to give the reader a timely overview of the most important and recent developments in this emerging class of therapeutically useful materials of DNA‐based hydrogels.
Tailoring Aerogels and Related 3D Macroporous Monoliths for Interfacial Solar Vapor Generation
Authors: Hu Xiaozhen , Zhu Jia 
Published: November 2019

AbstractInterfacial solar vapor generation is emerging as a promising water treatment technology with high solar energy efficiency and minimized carbon footprint. Among various kinds of materials development, aerogels, with inherent high porosity, lightweight, enhanced absorption, and minimized thermal conductivity, are attracting significant attention for achieving high‐performance solar vapor generation. Herein, recent progress in tailoring aerogels (such as graphene [oxide], carbon nanotubes, and polymer aerogels) and related 3D macroporous architectures for interfacial solar vapor generation is presented. Furthermore, the challenges and opportunities associated with employing aerogels in solar vapor generation are also discussed.
Void‐Free 3D Bioprinting for In Situ Endothelialization and Microfluidic Perfusion
Authors: Ouyang Liliang , Armstrong James P. K. , Chen Qu , Lin Yiyang , Stevens Molly M. 
Published: November 2019

AbstractTwo major challenges of 3D bioprinting are the retention of structural fidelity and efficient endothelialization for tissue vascularization. Both of these issues are addressed by introducing a versatile 3D bioprinting strategy, in which a templating bioink is deposited layer‐by‐layer alongside a matrix bioink to establish void‐free multimaterial structures. After crosslinking the matrix phase, the templating phase is sacrificed to create a well‐defined 3D network of interconnected tubular channels. This void‐free 3D printing (VF‐3DP) approach circumvents the traditional concerns of structural collapse, deformation, and oxygen inhibition, moreover, it can be readily used to print materials that are widely considered “unprintable.” By preloading endothelial cells into the templating bioink, the inner surface of the channels can be efficiently cellularized with a confluent endothelial layer. This in situ endothelialization method can be used to produce endothelium with a far greater cell seeding uniformity than can be achieved using the conventional postseeding approach. This VF‐3DP approach can also be extended beyond tissue fabrication and toward customized hydrogel‐based microfluidics and self‐supported perfusable hydrogel constructs.
Atomic Layer Deposition on Dispersed Materials in Liquid Phase by Stoichiometrically Limited Injections
Authors: Le Monnier Benjamin P. , Wells Frederick , Talebkeikhah Farzaneh , Luterbacher Jeremy S. 
Published: November 2019

AbstractAtomic layer deposition (ALD) is a well‐established vapor‐phase technique for depositing thin films with high conformality and atomically precise control over thickness. Its industrial development has been largely confined to wafers and low‐surface‐area materials because deposition on high‐surface‐area materials and powders remains extremely challenging. Challenges with such materials include long deposition times, extensive purging cycles, and requirements for large excesses of precursors and expensive low‐pressure equipment. Here, a simple solution‐phase deposition process based on subsequent injections of stoichiometric quantities of precursor is performed using common laboratory synthesis equipment. Precisely measured precursor stoichiometries avoid any unwanted reactions in solution and ensure layer‐by‐layer growth with the same precision as gas‐phase ALD, without any excess precursor or purging required. Identical coating qualities are achieved when comparing this technique to Al2O3 deposition by fluidized‐bed reactor ALD (FBR‐ALD). The process is easily scaled up to coat >150 g of material using the same inexpensive laboratory glassware without any loss in coating quality. This technique is extended to sulfides and phosphates and can achieve coatings that are not possible using classic gas‐phase ALD, including the deposition of phosphates with inexpensive but nonvolatile phosphoric acid.