bluuebunny/crossref_metadata_2025_split huggingface dataset:
Substrate Independent Liquid Phase Epitaxy of HKUST‐1 as Anticoagulant and Antimicrobial Coating
Authors: Zhang Yu , Zhao Qian , Li Weijie , Liu Junfeng , Chen Junying , Fan Yonghong , Weng Yajun 
Published: April 2020

AbstractThe effective deposition of metal–organic frameworks on kinds of biomaterials is still challenging and highly desirable. Using polydopamine as the effective nucleation center, HKUST‐1 is successfully prepared on three kinds of inert biomaterials, i.e., titanium oxide film (Ti–O), 316L stainless steel (316L SS), and poly(vinyl chloride) (PVC). Characterization of the surfaces by powder X‐ray diffraction confirm the oriented crystal growth of HKUST‐1 on the polydopamine coated substrates. There are no significant difference in catalytical NO generation and surface copper content of the three samples with HKUST‐1 deposition. All keep a NO release speed of ≈2.5 × 10−10 mol cm−2 min−1, thus platelet activation on the HKUST‐1 modified surfaces are inhibited compared to that of the unmodified ones. Ex vivo test shows thrombi formation is dramatically reduced. The HKUST‐1 coated surfaces elicit 95% reduction in the bacterial attachment of both Staphylococcus aureus and Escherichia coli. The endothelial cells and macrophages culture results support good biocompatibility of the modified surfaces. It provides a promising, facile, and effective approach for surface modification of blood contact biomaterials.
High‐Purity Hybrid Structural Colors by Enhancing Optical Absorption of Organic Dyes in Resonant Cavity
Authors: Yang Zhengmei , Ji Chengang , Cui Qingyu , Guo Lingjie Jay 
Published: April 2020

AbstractThis work presents a novel approach of incorporating an ultrathin dye film into a classic dielectric‐absorber‐dielectric‐metal resonator configuration for generating high‐purity reflective structural colors. Utilizing a thin film of organic dye having the same color as the targeted reflection color as a part of the cavity layer in the structure, its absorption at complementary color wavelengths is significantly enhanced due to the strong cavity resonances, hence reflection at the unwanted wavelengths strongly suppressed, leading to the improved purity of the desired reflective color. This design principle can be applied to create essentially all colors, and is demonstrated by experiment to produce high‐purity blue and red colors. In addition, the fabricated device exhibits outstanding stability under UV exposure without additional protections compared to traditional organic pigments. The proposed method in this work largely simplifies the design process of high‐purity structural colors, which paves the way for more potential applications in various fields.
A Pulsatile Flow System to Engineer Aneurysm and Atherosclerosis Mimetic Extracellular Matrix
Authors: Hosseini Vahid , Mallone Anna , Mirkhani Nima , Noir Jerome , Salek Mehdi , Pasqualini Francesco Silvio , Schuerle Simone , Khademhosseini Ali , Hoerstrup Simon P. , Vogel Viola 
Published: April 2020

AbstractAlterations of blood flow patterns strongly correlate with arterial wall diseases such as atherosclerosis and aneurysm. Here, a simple, pumpless, close‐loop, easy‐to‐replicate, and miniaturized flow device is introduced to concurrently expose 3D engineered vascular smooth muscle tissues to high‐velocity pulsatile flow versus low‐velocity disturbed flow conditions. Two flow regimes are distinguished, one that promotes elastin and impairs collagen I assembly, while the other impairs elastin and promotes collagen assembly. This latter extracellular matrix (ECM) composition shares characteristics with aneurysmal or atherosclerotic tissue phenotypes, thus recapitulating crucial hallmarks of flow‐induced tissue morphogenesis in vessel walls. It is shown that the mRNA levels of ECM of collagens and elastin are not affected by the differential flow conditions. Instead, the differential gene expression of matrix metalloproteinase (MMP) and their inhibitors (TIMPs) is flow‐dependent, and thus drives the alterations in ECM composition. In further support, treatment with doxycycline, an MMP inhibitor and a clinically used drug to treat vascular diseases, halts the effect of low‐velocity flow on the ECM remodeling. This illustrates how the platform can be exploited for drug efficacy studies by providing crucial mechanistic insights into how different therapeutic interventions may affect tissue growth and ECM assembly.
Copper‐Catalyzed Electrophilic Etherification of Arylboronic Esters with Isoxazolidines
Authors: Katahara Seiya , Takahashi Tenga , Nomura Kengo , Uchiyama Masanobu , Sato Takaaki , Chida Noritaka 
Published: April 2020

AbstractA copper‐catalyzed electrophilic etherification of arylboronic esters is reported. Isoxazolidines are utilized as easily available and stable [RO]+ surrogates to give 1,3‐amino aryl ethers. The O‐selective arylation of isoxazolidines takes place without causing competitive N‐arylation. In contrast to previously reported anionic conditions, our copper‐catalyzed conditions are mild enough to achieve high functional group tolerance. Preliminary mechanistic studies and DFT calculations support that the reaction proceeds via a transmetalation/oxidative addition pathway, followed by a Lewis acid‐promoted reductive elimination to induce the crucial O‐selectivity.
Luminescence Enhancement based Sensing of L‐Cysteine by Doped Quantum Dots
Authors: Pramanik Sabyasachi , Roy Shilaj , Bhandari Satyapriya 
Published: April 2020

AbstractThe interaction of a presynthesized orange emitting Mn2+‐doped ZnS quantum dots (QDs) with L‐Cysteine (L−Cys) led to enhance emission intensity (at 596 nm) and quantum yield (QY). Importantly, the Mn2+‐doped ZnS QDs exhibited high sensitivity towards L−Cys, with a limit of detection of 0.4±0.02 μM (in the linear range of 3.3–13.3 μM) and high selectivity in presence of interfering amino acids and metal ions. The association constant of L−Cys was determined to be 0.36×105 M−1. The amplified passivation of the surface of Mn2+‐doped ZnS QDs following the incorporation and binding of L−Cys is accounted for the enhancement in their luminescence features. Moreover, the luminescence enhancement‐based detection will bring newer dimension towards sensing application.
Cloning, characterization, and enzymatic identification of a new tryptophan decarboxylase from Ophiorrhiza pumila
Authors: You Dawei , Feng Yue , Wang Can , Sun Chengtao , Wang Yao , Zhao Degang , Kai Guoyin 
Published: April 2020

AbstractTryptophan decarboxylase (TDC, EC 4.1.1.28) catalyzes tryptophan decarboxylation to form tryptamine through the cofactor pyridoxal‐5′‐phosphate (PLP), a crucial stage in the production of the terpenoid indole alkaloids like camptothecin (CPT). A new gene encoding TDC was identified from the CPT‐producing plant Ophiorrhiza pumila by transcriptome analysis, termed OpTDC2. It contained a 1,536 bp open reading frame that encodes a 511 amino acid protein with a molecular mass of 57.01 kDa and an isoelectric point of 6.39. Multiple sequence alignment and phylogenetic tree analysis showed the closest similarity (85%) with the TDC from Mitragyna speciosa. Moreover, the highest expression of OpTDC2 was observed in the O. pumila root. To achieve high‐efficiency expression of OpTDC2 in Escherichia coli, we fused the TF tag onto the N‐terminal of the OpTDC2. Optimum enzymatic activity was observed at 45 °C, pH 8 and cofactor concentration of 0.1 mM. The catalytic reaction was strongly inhibited by metal ions of Cu2+, Zn2+, and Fe2+. The l‐tryptophan was particularly catalyzed compared with d‐tryptophan. Besides, the Km and kcat of the OpTDC2 were 1.08 mM and 0.78 Sec−1, respectively. The results provided information on new functional OpTDC2 that might be used in synthetic biology for the enhanced biosynthesis of CPT in O. pumila.
Thin‐layer chromatography/fluorescence detection approach for sensitive and selective determination of hepatitis C virus antiviral (velpatasvir): application to human plasma
Authors: Derayea Sayed M. , Abdel‐Lateef Mohamed A. , Omar Mahmoud A. , Ali Ramadan 
Published: April 2020

AbstractA validated thin‐layer chromatography (TLC) method combined with fluorescence detection mode was developed for the selective determination of a recently approved anti‐hepatitis C virus (HCV) drug (velpatasvir). The separation was performed on silica gel 60 F254 plates using ethylacetate:methanol:triethylamine (48:1.5:1.0, v/v/v) as a mobile phase. Plates were scanned in the fluorescence mode after excitation at 335 nm. This method provided an excellent separation of velpatasvir from sofosbuvir with RF values of 0.22 and 0.46 for velpatasvir and sofosbuvir, respectively, after scanning the developed plates in the ultraviolet detection mode at 335 nm. The calibration curve was linear over the range 4–40 ng/band with a correlation coefficient of 0.9994. The developed procedure was validated according to ICH guidelines with a detection limit of 1.30 ng/band and quantitation limit of 3.95 ng/band. The suggested method could selectively determine velpatasvir with high sensitivity in a synthetic tablet powder containing a co‐formulated anti‐HCV drug (sofosbuvir) without any interference from excipients or sofosbuvir. In addition, the method was successfully applied for determination of velpatasvir in spiked human plasma with adequate % recovery.
Middle interhemispheric variant of holoprosencephaly: First prenatal report of a ZIC2 missense mutation
Authors: Gounongbé Caroline , Marangoni Martina , Gouder de Beauregard Vanessa , Delaunoy Mélanie , Jissendi Patrice , Cassart Marie , Désir Julie 
Published: April 2020

AbstractWe present a case of a middle interhemispheric variant of antenatal discovery associated with a de novo missense variant (NM_007129.5: c.1109G>A p.(Cys370Tyr)) in the ZIC2 gene. Our case represents the first prenatal description of a ZIC2 missense mutation found in association with syntelencephaly.
Herpes zoster in an 11‐month‐old immunocompetent infant: A rare case report
Authors: Sah Chandra Mohan , Shrestha Sandeep , Chaudhary Nagendra 
Published: April 2020

AbstractHerpes zoster generally occurs after the exposure of VZV following varicella. This case report emphasizes a rare case of HZ in an infant without prior history of varicella or its exposure in the baby, other siblings, and mother during or after the pregnancy.
Generation of a Key Synthon of Indole Alkaloid Synthesis by Palladium(II)‐Catalyzed Indole 2‐Methylenephosphorylation
Authors: Niu Yuan , Bai Peng‐Bo , Lou Qin‐Xin , Yang Shang‐Dong 
Published: April 2020

AbstractA palladium‐catalyzed Catellani‐type reaction of indole with diethyl (iodomethyl)phosphonate has been developed. This new protocol provides an efficient and concise route to 2‐methylene phosphonate indoles, which are key synthons for the construction of indole alkaloids.
Palladium/Copper‐catalyzed Oxidation of Aliphatic Terminal Alkenes to Aldehydes Assisted by p‐Benzoquinone
Authors: Komori Saki , Yamaguchi Yoshiko , Murakami Yuka , Kataoka Yasutaka , Ura Yasuyuki 
Published: April 2020

AbstractThe development of an anti‐Markovnikov Wacker‐type oxidation for simple aliphatic alkenes is a significant challenge. Herein, a variety of aldehydes can be selectively obtained from various unbiased aliphatic terminal alkenes using PdCl2(MeCN)2/CuCl in the presence of p‐benzoquinone (BQ) under mild reaction conditions. Isomerization of the terminal alkene to the internal alkene was suppressed via slow addition of the starting material to the reaction mixture. In addition to the Pd catalyst, CuCl and BQ were essential in order to obtain the anti‐Markovnikov product with high selectivity. Terminal alkenes bearing a halogen substituent afforded their corresponding aldehydes with high anti‐Markovnikov selectivity. The halogen acts as a directing group in the reaction. DFT calculations indicate that a μ‐chloro Pd(II)−Cu(I) bimetallic species with BQ coordinated to Cu is the catalytically active species in the case of a terminal alkene without a directing group.
Flexibility Potential of Photovoltaic Power Plant and Biogas Plant Hybrid Systems in the Distribution Grid
Authors: Bär Katharina , Wageneder Stefanie , Solka Felix , Saidi Abdessamad , Zörner Wilfried 
Published: April 2020

AbstractThe potential of combining biogas and photovoltaic (PV) power plants in hybrid systems in the German distribution grid is analyzed. The focus of the present research is on balancing the intermittent power supply from PV power plants with the controllable power production of combined heat and power (CHP) units of biogas plants within a period of seconds. To achieve an increase of the total energy feed‐in of biogas and PV power plants to the electricity grid, a biogas plant energy management system is described. System parameters, such as the variable feed‐in of PV power plants or power ramps of the start‐up process of controllable biogas plant CHP unit, are described and adapted to the global installed capacity of PV power plant and biogas plants in the German distribution grid.
Reproducibility of “the Papanicolaou Society of Cytopathology system for reporting respiratory cytology” – A retrospective analysis of 101 cases of CT‐guided FNAC
Authors: Boler Anup Kumar , Banu Nasreen , Bose Koushik , Roy Shreosee , Bandyopadhyay Arghya 
Published: April 2020

AbstractIntroductionGuided fine‐needle aspiration cytology is a popular investigative procedure in diagnosing pulmonary lesions. The Papanicolaou Society of Cytopathology (PSC) has already outlined a categorical system for reporting respiratory cytology. Though each category has a known malignancy risk, their inter observer reproducibility have not been well documented. This study was directed towards establishing the reproducibility of this categorical system in diagnosing pulmonary lesions.MethodOne hundred and one consecutive cytology specimens obtained by CT‐guided FNA from lung lesions were independently reviewed by 3 experienced cytopathologists, who allotted each case to 1 of 6 PSC categories. Statistical analysis for percent overall agreement was done using Fleiss' Kappa.ResultPercent overall agreement was 71.29% and free marginal kappa was 0.66. On combining categories “suspicious” and “malignant” percent overall agreement was 79.54% and free marginal kappa was 0.74.ConclusionThere was substantial agreement among the observers as regards reproducibility of categories which can improve if we combine certain categories, especially “suspicious” and “malignant.”
Effect of tower base painting on willow ptarmigan collision rates with wind turbines
Authors: Stokke Bård G. , Nygård Torgeir , Falkdalen Ulla , Pedersen Hans C. , May Roel 
Published: April 2020

Abstract

Birds colliding with turbine rotor blades is a well‐known negative consequence of wind‐power plants. However, there has been far less attention to the risk of birds colliding with the turbine towers, and how to mitigate this risk.

Based on data from the Smøla wind‐power plant in Central Norway, it seems highly likely that willow ptarmigan (the only gallinaceous species found on the island) is prone to collide with turbine towers. By employing a BACI‐approach, we tested if painting the lower parts of turbine towers black would reduce the collision risk.

Overall, there was a 48% reduction in the number of recorded ptarmigan carcasses per search at painted turbines relative to neighboring control (unpainted) ones, with significant variation both within and between years.

Using contrast painting to the turbine towers resulted in significantly reduced number of ptarmigan carcasses found, emphasizing the effectiveness of such a relatively simple mitigation measure.
Two cases of successful treatment of acute right heart failure with Impella RP®
Authors: Santer David , Boltres Anita , Koechlin Luca , Hunziker Patrick , Jeger Raban , Maurer Markus , Grapow Martin , Eckstein Friedrich , Reuthebuch Oliver 
Published: April 2020

AbstractPost‐operative right coronary artery occlusion is a serious complication that demands acute coronary revascularization to prevent myocardial infarction. We present two cases with acute right coronary artery obstruction caused by (1) transfemoral aortic valve implantation and (2) acute type A aortic dissection. Although coronary artery bypass grafting was performed intraoperatively, right heart failure was observed in both cases. The Impella RP® device offers temporary right ventricular mechanical support; wherefore, we decided to deploy it in both patients. The devices were uneventfully and successfully implanted to bridge for recovery of the right heart. We report the perioperative course of the patients as well as their condition at 1 year follow‐up.
Mathematical modeling for prilling processes in ammonium nitrate production
Authors: Gezerman Ahmet Ozan 
Published: April 2020

AbstractIn ammonium nitrate (AN) production, determining the standard particle size for the granulation process is a fundamental problem. Therefore, a system model is proposed for this final stage of the manufacturing process. In the proposed model, prilling is simulated by analyzing data from the continuity, hydrodynamics, and mass and energy transfers. The direction of the movement of AN particles, the temperature, and humidity distribution of the cooling air with variation in particle size, and the height of the prilling tower are calculated. The air temperature profile determined by the model is compared with that of an actual AN plant. The comparison shows that the results obtained by the model are consistent with the plant data. In addition, it is shown that an increase in the cooling rate of particles has the potential to enhance the properties of AN particles. Moreover, a detailed analysis of the granulation process is conducted to estimate the impact of the parameters of the proposed model such as the prilling tower height, particle size, and temperature. Thus, this model could be used to obtain more accurate results at an industrial plant.
Modelling Enzymatic Mechanisms with QM/MM Approaches: Current Status and Future Challenges
Authors: Magalhães Rita P. , Fernandes Henriques S. , Sousa Sérgio F. 
Published: April 2020

AbstractQuantum mechanics/molecular mechanics (QM/MM) methods are presently a well‐established alternative for the study of enzymatic reaction mechanisms. They enable the description of a small part of the enzyme, where reactions take place through QM, while the majority of the thousands of atoms that comprise these biomolecules are handled through MM. While different “flavors” and variations in the QM/MM field exist, this review will focus more on the application of the ONIOM methodology, presenting a fresh perspective on the application of this popular method in light of the growth in computational power and level of sophistication of the different methodologies that it can combine. In addition to a brief presentation of the basic principles behind these methods, this review will discuss different examples of applicability, common choices, practical considerations, and main problems involved, stemming from our experience in this field. Finally, a reflection on the future challenges for the next decade in the QM/MM modeling of enzymatic mechanisms is presented.
Quantitative proteomic analysis to determine differentially expressed proteins in axenic amastigotes of Leishmania tropica and Leishmania major
Authors: Ashrafmansouri Marzieh , Amiri‐Dashatan Nasrin , Ahmadi Nayebali , Rezaei‐Tavirani Mostafa , SeyyedTabaei Seyyedjavad , Haghighi Ali 
Published: April 2020

AbstractCutaneous leishmaniasis is commonly caused by Leishmania major and Leishmania tropica. In the present study, the differential expression of proteins was identified in the amastigote‐like forms of L. tropica and L. major in Iranian isolates. Initially, the samples were cultured and identified using PCR‐RFLP technique. The Leishmania isolates were then grown in host‐free (axenic) culture and prepared to amastigote‐like forms, followed by the extraction of their proteins. To identify significant differentially expressed proteins (DEPs) of two types of Leishmania, the label‐free quantitative proteomic technique was used based on sequential window acquisition of all theoretical fragment ion spectra mass spectrometry. A total of 51 up/down‐DEPs (fold change >2 and p‐value <.05) were identified between the axenic amastigote forms of L. major and L. tropica. Of these, 34 and 17 proteins were up‐regulated in L. major and L. tropica, respectively. Several enriched GO terms were identified via biological process analyses for DEPs; furthermore, the metabolic process and translation were disclosed as top category in the up‐regulated proteins of both L. major and L. tropica species. Also, the KEGG analysis revealed carbon metabolism and metabolic pathways term as the top pathways in the proteins up‐regulated in L. major and L. tropica, respectively. Taken together, the numerous novel DEPs identified between the studied species could help fully understand the molecular mechanisms of pathogenesis and provide potential drug targets and vaccine candidates.
Evaluating the impact of a 15‐year academic partnership to promote sustainable engagement, education, and scholarship in global health
Authors: Karwa Rakhi , Miller Monica L. , Schellhase Ellen , Tran Dan , Manji Imran , Njuguna Benson , Fletcher Sara , Kanyi John , Maina Mercy , Jakait Beatrice , Kigen Gabriel , Kipyegon Victor , Aruasa Wilson , Crowe Susie , Pastakia Sonak D. 
Published: April 2020

AbstractIntroductionIn 2003, Purdue University College of Pharmacy (PUCOP) in West Lafayette, Indiana, began the Purdue Kenya Partnership (PKP) in collaboration with the Academic Model Providing Access to Healthcare, Moi University, and Moi Teaching and Referral Hospital, in Eldoret, Kenya. PUCOP's involvement utilized a tripartite approach of engagement, education, and scholarship to provide and expand sustainable access to high quality care.ObjectiveThis paper discusses outcomes and impacts of this academic partnership.MethodsPurdue Kenya Partnership's progress in achieving its stated mission was evaluated using an outcome‐approach logic model. This model highlighted inputs, activities, and results which encompassed outputs, outcomes, and impact. A comprehensive set of ratios were calculated to quantify annual change in PKP investments against estimated metrics for engagement, education, and scholarship. These metrics were weighted by involvement level and pharmacist effort in various clinical domains. Descriptive statistics were completed that identified cumulative and totals per year for each collected data type of data collected.ResultsPurdue Kenya Partnership implementation utilized initial inputs of human resources, financial capital, and strategic partnerships. These inputs supported pharmacy involvement in 16 distinct care programs in both inpatient and outpatient settings which supported the care of 457 833 individual patients and grown a clinical pharmacy staff from 0 to 22 practicing clinical pharmacists. Five unique educational programs have been established which have graduated 457 trainees. Purdue Kenya Partnership has generated over $6.2 million in grant funding and disseminated 302 peer reviewed manuscripts, posters, and oral presentations combined. Ratios describing trends in engagement, education, and scholarship as a result of using the locally focused PKP approach highlight higher initial costs compared with much lower costs per outcome several years into the partnership.ConclusionThe PKP's global health approach of prioritizing the population's care needs (“leading with care”) has enabled the development of sustainable engagement, education, and scholarship infrastructure with significant gains in all three domains.