bluuebunny/crossref_metadata_2025_split huggingface dataset:
International prognostic score for asymptomatic early-stage chronic lymphocytic leukemia
Authors: Condoluci Adalgisa , Terzi di Bergamo Lodovico , Langerbeins Petra , Hoechstetter Manuela A. , Herling Carmen D. , De Paoli Lorenzo , Delgado Julio , Rabe Kari G. , Gentile Massimo , Doubek Michael , Mauro Francesca R. , Chiodin Giorgia , Mattsson Mattias , Bahlo Jasmin , Cutrona Giovanna , Kotaskova Jana , Deambrogi Clara , Smedby Karin E. , Spina Valeria , Bruscaggin Alessio , Wu Wei , Moia Riccardo , Bianchi Elena , Gerber Bernhard , Zucca Emanuele , Gillessen Silke , Ghielmini Michele , Cavalli Franco , Stussi Georg , Hess Mark A. , Baumann Tycho S. , Neri Antonino , Ferrarini Manlio , Rosenquist Richard , Forconi Francesco , Foà Robin , Pospisilova Sarka , Morabito Fortunato , Stilgenbauer Stephan , Döhner Hartmut , Parikh Sameer A. , Wierda William G. , Montserrat Emili , Gaidano Gianluca , Hallek Michael , Rossi Davide 
Published: April 2020

AbstractMost patients with chronic lymphocytic leukemia (CLL) are diagnosed with early-stage disease and managed with active surveillance. The individual course of patients with early-stage CLL is heterogeneous, and their probability of needing treatment is hardly anticipated at diagnosis. We aimed at developing an international prognostic score to predict time to first treatment (TTFT) in patients with CLL with early, asymptomatic disease (International Prognostic Score for Early-stage CLL [IPS-E]). Individual patient data from 11 international cohorts of patients with early-stage CLL (n = 4933) were analyzed to build and validate the prognostic score. Three covariates were consistently and independently correlated with TTFT: unmutated immunoglobulin heavy variable gene (IGHV), absolute lymphocyte count higher than 15 × 109/L, and presence of palpable lymph nodes. The IPS-E was the sum of the covariates (1 point each), and separated low-risk (score 0), intermediate-risk (score 1), and high-risk (score 2-3) patients showing a distinct TTFT. The score accuracy was validated in 9 cohorts staged by the Binet system and 1 cohort staged by the Rai system. The C-index was 0.74 in the training series and 0.70 in the aggregate of validation series. By meta-analysis of the training and validation cohorts, the 5-year cumulative risk for treatment start was 8.4%, 28.4%, and 61.2% among low-risk, intermediate-risk, and high-risk patients, respectively. The IPS-E is a simple and robust prognostic model that predicts the likelihood of treatment requirement in patients with early-stage CLL. The IPS-E can be useful in clinical management and in the design of early intervention clinical trials.
Identification of NHXs in Gossypium species and the positive role of GhNHX1 in salt tolerance
Authors: Long Lu , Zhao Jing-Ruo , Guo Dan-Dan , Ma Xiao-Nan , Xu Fu-Chun , Yang Wen-Wen , Gao Wei 
Published: April 2020

Abstract
Background
Plant Na+/H+ antiporters (NHXs) are membrane-localized proteins that maintain cellular Na+/K+ and pH homeostasis. Considerable evidence highlighted the critical roles of NHX family in plant development and salt response; however, NHXs in cotton are rarely studied.

Results
The comprehensive and systematic comparative study of NHXs in three Gossypium species was performed. We identified 12, 12, and 23 putative NHX proteins from G. arboreum, G. raimondii, and G. hirsutum, respectively. Phylogenetic study revealed that repeated polyploidization of Gossypium spp. contributed to the expansion of NHX family. Gene structure analysis showed that cotton NHXs contain many introns, which will lead to alternative splicing and help plants to adapt to high salt concentrations in soil. The expression changes of NHXs indicate the possible differences in the roles of distinct NHXs in salt response. GhNHX1 was proved to be located in the vacuolar system and intensively induced by salt stress in cotton. Silencing of GhNHX1 resulted in enhanced sensitivity of cotton seedlings to high salt concentrations, which suggests that GhNHX1 positively regulates cotton tolerance to salt stress.

Conclusion
We characterized the gene structure, phylogenetic relationship, chromosomal location, and expression pattern of NHX genes from G. arboreum, G. raimondii, and G. hirsutum. Our findings indicated that the cotton NHX genes are regulated meticulously and differently at the transcription level with possible alternative splicing. The tolerance of plants to salt stress may rely on the expression level of a particular NHX, rather than the number of NHXs in the genome. This study could provide significant insights into the function of plant NHXs, as well as propose promising candidate genes for breeding salt-resistant cotton cultivars.
Development and characterization of 16 novel microsatellite markers by Transcriptome sequencing for Angelica dahurica and test for cross-species amplification
Authors: Liu Qianqian , Lu Zuyu , He Wei , Li Fang , Chen Wenna , Li Chan , Chao Zhi , Tian Enwei 
Published: April 2020

Abstract
Background
Angelica dahurica (Apiaceae) is an important herb in traditional Chinese medicine. Because of its important medicinal and economic values, its wild resources were over-exploited and increasingly reduced. Meanwhile, the diversity of cultivars of A. dahurica has decreased as a result of long-term artificial cultivation. However, there are no population genetics studies of natural A. dahurica reported yet, especially for using microsatellite markers (SSRs) to investigate population genetics of the species.

Results
Sixteen polymorphic EST-SSR loci were isolated from A. dahurica with transcriptome sequencing technology (RNA-Seq). The number of alleles varied from 2 to 15 per polymorphic locus over populations with the observed and expected heterozygosities ranging from 0.000 to 1.000 and from 0.000 to 0.829, respectively. Significant deviations from Hardy–Weinberg equilibrium were observed at 8 loci. Tests of linkage disequilibrium showed 11 informative locus pairs were significant across all populations. Cross-species amplification showed that 14 out of 16 SSR loci have the transferability in cultivar-A. dahurica cv. ‘Hangbaizhi’ and A. decursiva.

Conclusions
The 16 newly developed loci microsatellite primers with RNA-Seq will be useful for further investigating population genetics of A. dahurica, cultivars and other members of this genus.
Minimum 8-year follow-up of revision THA with severe femoral bone defects using extensively porous-coated stems and cortical strut allografts
Authors: Ding Zi-chuan , Ling Ting-xian , Yuan Ming-cheng , Qin Yong-zhi , Mou Ping , Wang Hao-yang , Zhou Zong-ke 
Published: April 2020

AbstractBackgroundRevision total hip arthroplasty (THA) with severe femoral bone defects remains a major challenge. The purpose of this study is to report the minimum 8-year clinical and radiographic results of revision THA with severe femoral bone defects treated with extensively porous-coated stems and cortical strut allografts.MethodsWe retrospectively identified 44 patients diagnosed with Paprosky type III and IV femoral bone defects between January 2006 and July 2011. The exclusion criteria were patients not eligible for surgery, revised with extensively porous-coated stems alone, lost to follow-up and deceased. A total of 31 patients treated with extensively porous-coated stems and cortical strut allografts were finally included in this study. The degree of femoral bone defects was categorized as Paprosky type IIIA in 19 patients, type IIIB in 9 patients and type IV in 3 patients. The mean duration of follow-up was 11.0 ± 1.5 (range, 8.1–13.5) years.ResultsThe mean Harris Hip Score improved significantly from 43.4 ± 10.5 points to 85.2 ± 6.6 points (P < 0.001). Similarly, WOMAC and SF-12 scores also significantly improved. Twenty-eight stems achieved stable bone ingrowth, two stems showed stable fibrous ingrowth, and one stem was radiologically unstable. Complete union and bridging between cortical strut allografts and host bone was achieved in all 31 patients. The femoral width was augmented with cortical strut allografts after revision surgery (an increase of 10.5 ± 0.5 mm) and showed a slight decrease of 2.5 ± 4.8 mm after the 10-year follow-up. Using re-revision for any reason as an endpoint, the Kaplan-Meier cumulative survival rate of the stem was 96.2% (95% confidence interval, 75.7–99.5%) at 10 years.ConclusionOur data demonstrate that the use of extensively porous-coated stems combined with cortical strut allografts in revision THA with Paprosky type III and IV femoral bone defects can provide satisfactory clinical and radiographic outcomes with a minimum follow-up of 8 years.
A trial-based economic evaluation of the Restore4Stroke self-management intervention compared to an education-based intervention for stroke patients and their partners
Authors: van Mastrigt Ghislaine A. P. G. , van Eeden Mitchel , van Heugten Caroline M. , Tielemans Nienke , Schepers Vera P. M. , Evers Silvia M. A. A. 
Published: April 2020

Abstract
Background
Since stroke survivors are increasingly responsible for managing stroke-related changes in their own health and lifestyle, self-management skills are required. In a recent randomised controlled trial a self-management intervention based on proactive coping action planning (SMI) in comparison with an education-based intervention (EDU) in stroke patients was investigated. However, no relevant treatment effects on the Utrecht Proactive Coping Competence scale (UPCC) and the Utrecht Scale for Evaluation of Rehabilitation Participation (USER-Participation) were found. The current study is a trial-based economic evaluation from a societal perspective comparing the same interventions (SMI versus EDU).

Methods
UPCC, USER-Participation and EuroQol (EQ-5D-3 L) and costs were measured at baseline, three, six and twelve months after treatment. For the cost-effectiveness analyses, incremental cost effectiveness ratios (ICERs) were calculated for UPCC and USER-Participation. For the cost-utility analyses the incremental cost utility ratio (ICUR) was expressed in cost per Quality Adjusted Life Years (QALYs). Outcomes were tested by means of AN(C)OVA analyses and costs differences by means of bootstrapping. Bootstrapping, sensitivity analyses and a subgroup analysis were performed to test the robustness of the findings.

Results
One hundred thirteen stroke patients were included in this study. The mean differences in USER-Participation scores (95%CI:-13.08,-1.61, p-value = .013) were significant different between the two groups, this does not account for UPCC scores (95%CI:-.267, .113, p-value = not significant) and QALYs (p-value = not significant) at 12 months. The average total societal costs were not significantly different (95%CI:€-3380,€7099) for SMI (€17,333) in comparison with EDU (€15,520). Cost-effectiveness analyses showed a mean ICER of 26,514 for the UPCC and 346 for the USER-Participation. Cost-utility analysis resulted in an ICUR of €44,688 per QALY. Assuming a willingness to pay (WTP) threshold of €50,000 per QALY, the probability that SMI will be cost-effective is 52%. Sensitivity analyses and subgroup analysis showed the robustness of the results.

Conclusions
SMI is probably not a cost-effective alternative in comparison with EDU. Based on the current results, the value of implementing SMI for a stroke population is debatable. We recommend further exploration of the potential cost-effectiveness of stroke-specific self-management interventions focusing on different underlying mechanisms and using different control treatments.
Reporting of financial conflicts of interest in meta-analyses of drug trials published in high-impact medical journals: comparison of results from 2017 to 2018 and 2009
Authors: Benea Carla , Turner Kimberly A. , Roseman Michelle , Bero Lisa A. , Lexchin Joel , Turner Erick H. , Thombs Brett D. 
Published: April 2020

Abstract
Background
A previous study found that 2 of 29 (6.9%) meta-analyses published in high-impact journals in 2009 reported included drug trials’ funding sources, and none reported trial authors’ financial conflicts of interest (FCOIs) or industry employment. It is not known if reporting has improved since 2009. Our objectives were to (1) investigate the extent to which pharmaceutical industry funding and author-industry FCOIs and employment from included drug trials are reported in meta-analyses published in high-impact journals and (2) compare current reporting with results from 2009.

Methods
We searched PubMed (January 2017–October 2018) for systematic reviews with meta-analyses including ≥ 2 randomized controlled trials (RCTs) of patented drugs. We included 3 meta-analyses published January 2017–October 2018 from each of 4 high-impact general medicine journals, high-impact journals from 5 specialty areas, and the Cochrane Database of Systematic Reviews, as in the previous study.

Results
Among 29 meta-analyses reviewed, 13 of 29 (44.8%) reported the funding source of included trials compared to 2 of 29 (6.9%) in 2009, a difference of 37.9% (95% confidence interval, 15.7 to 56.3%); this included 7 of 11 (63.6%) from general medicine journals, 3 of 15 (20.0%) from specialty medicine journals, and 3 of 3 (100%) Cochrane reviews. Only 2 of 29 meta-analyses (6.9%) reported trial author FCOIs, and none reported trial author-industry employment.

Protocol Publication
A protocol was uploaded to the Open Science Framework prior to initiating the study. https://osf.io/8xt5p/

Limitations
We examined only a relatively small number of meta-analyses from selected high-impact journals and compared results to a similarly small sample from an earlier time period.

Conclusions
Reporting of drug trial sponsorship and author FCOIs in meta-analyses published in high-impact journals has increased since 2009 but is still suboptimal. Standards on reporting of trial funding described in the forthcoming revised PRISMA statement should be adapted and enforced by journals to improve reporting.
Protective effect of asiaticoside on radiation-induced proliferation inhibition and DNA damage of fibroblasts and mice death
Authors: Shen Haiyan , Zhu Fei , Li Jinsheng , Tang Songjia , Zhang Yale , Zhang Jufang 
Published: April 2020

AbstractBackgroundRadiation-induced injuries (RII) mainly result from reactive oxygen species (ROS), which are harmful compounds that can damage DNA. Asiaticoside (AC), one of the main functional components extracted from Centella asiatica, has potent pharmacological effects such as anti-inflammatory and anti-oxidant activity. However, its role in RII remains unclear.PurposeThe purpose of the current study is to investigate whether AC can mitigate RII in vitro and in vivo.Material and MethodsCell model of RII was successfully established by 5J/m2 radiation in vitro. For the in vivo RII model, mice were irradiated with 5 Gy to the thorax. The degree of damage to cells or mouse tissue was determined by measuring the numbers of DNA double-strand breaks (DSBs), oxidative stress, and mouse survival rates.ResultsIn the in vitro assay, AC administration significantly reduced radiation-induced growth inhibition of Escherichia coli and fibroblasts, DSBs and apoptosis of fibroblasts; in the in vivo study, AC could decrease antioxidant capacity (T-AOC) of plasma and protect mice from RII, thereby improving the survival rates of mice after radiation.ConclusionsThese novel data indicate that AC is able to prevent radiation-initiated genotoxicity by mitigating DNA damage, and might serve as a safe and effective radio-protective agent.
Os Principais Assuntos de Auditoria Importam? Uma análise de sua associação com o Gerenciamento de Resultados
Authors: Santos Kleyverson Leonardo dos , Guerra Renan Bittencourt , Marques Vagner Antônio , Maria Júnior Elizeu 
Published: April 2020

Objetivo: Analisar a relação entre os Principais Assuntos de Auditoria (PAA´s) reportados e o nível de gerenciamento de resultados das empresas brasileiras.
Método: A amostra de pesquisa foi composta por 96 empresas listadas no IBRX 100 cujos dados foram coletados na CVM – Comissão de Valores Mobiliários e na base de dados ComDinheiro, no período compreendido entre 2016 e 2017, utilizando estatística descritiva, testes de diferenças entre as médias e análise de regressão com dados em painel.
Resultados: Os tipos de PAA´s predominantes foram: Recuperabilidade dos ativos, Contingências e Reconhecimento de receitas, respondendo, conjuntamente, nos anos de 2016 e 2017, por 58% e 66%, respectivamente. Observou-se uma associação positiva e significativa entre a quantidade de PAA´s e os accruals e receitas discricionárias e uma associação negativa e significativa entre a quantidade de PAA´s reportados e a proxy de gerenciamento de resultados pelas operações através das despesas discricionárias.
Contribuições: Os resultados têm implicações no debate sobre o tema, afetando as práticas de contadores, auditores e reguladores, pois apresenta insigths relevantes sobre a associação entre a divulgação de PAA´s e as proxies de Gerenciamento de Resultados pelos accruals e pelas operações reais, evidenciando a importância da divulgação dos PAA´s como red flag para manipulação de dados contábeis.
Determinantes da satisfação e lealdade dos discentes de programas de pós-graduação em Ciências Contábeis do Brasil: Um estudo a partir da perspectiva do European Customer Satisfaction Index (ECSI)
Authors: Câmara Arianne Raquel Axiole de Souza , Melo Clayton Levy Lima de , Mol Andersol Luiz Rezende , Melo Magaly Aparecida Galvão Dantas de 
Published: April 2020

Objetivo: analisar se o modelo de satisfação European Customer Satisfaction Index (ECSI) representa os determinantes da satisfação dos estudantes dos cursos de pós-graduação stricto sensu em Ciências Contábeis do Brasil.
Método: Trata-se de uma pesquisa do tipo survey com estudantes dos 29 programas stricto sensu em Ciências Contábeis em funcionamento no ano de 2017 que são avaliados pela CAPES. Foram coletadas 331 respostas de 26 PPGs que, após o tratamento dos dados, resultaram em 311 dados válidos, alcançando cerca de 90% dos programas ativos. As análises dos dados foram desenvolvidas mediante utilização da Modelagem de Equações Estruturais.
Resultados: O estudo atendeu aos critérios estatísticos para sua validação e com as reespecificações realizadas, o modelo conceitual de satisfação discente alcançou adequados índices de ajustamento de qualidade e se mostrou estável, e adequado diante da amostra estudada, explicando 96,6% da variação da lealdade e 80,6% da satisfação dos pós-graduandos.
Contribuições: Almeja-se que, ao apresentar o modelo estrutural que retrata os determinantes da satisfação discente, contribuir para que os discentes vivam o aprendizado acadêmico com elevado grau de satisfação, obtenham um bom desempenho e que isso reflita em uma avaliação positiva para a instituição, bem como para área de ensino da Contabilidade.
Kethoxal-assisted single-stranded DNA sequencing (KAS-seq) for capturing transcription dynamics and enhancer activity
Authors: Wu Tong , Lyu Ruitu , He Chuan 
Published: April 2020

Abstract
We describe a rapid and sensitive labeling of single-stranded DNA for sequencing (KAS-seq), based on a kethoxal-guanine reaction. KAS-seq procedure involves N3-kethoxal labeling, DNA isolation, biotinylation, fragmentation and enrichment, library preparation and sequencing. The whole process can be finished in a day. KAS-seq enables rapid measurement of the dynamics of transcriptionally-engaged Pol II, transcribing enhancers, Pol I and Pol III activities, and non-canonical DNA structures involving ssDNA simultaneously in situ, by using as few as 1,000 cells or mice tissues. The robust and tissue-friendly nature coupled with low input material requirement make KAS-seq a method that can be broadly applied. This step-by-step protocol is related to the publication “Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ” in Nature Methods.
The correlation and clinical significance of PIK3CA, PIK3CB and HPV infection in esophageal squamous cell carcinoma
Authors: Wen Feifei , Li Yangyang , He Shuang , Xu Xiaoyang , Liu Yuanhang , Liu Liu , Guo Ningjie , Jia Zhenzhen , Liu Haiqing , Shu Xiangfang , Wu Shuhua 
Published: April 2020

Abstract
Background: Esophageal squamous cell carcinoma (ESCC) is a highly lethal cancer. Currently, human papillomavirus (HPV) is considered as a potential risk factor for ESCC, but this assumption is still contradictory. PI3K/Akt/mTOR network plays a key role in the virus/host cell crosstalk in HPV positive cancer cells. The purpose of this study was to investigate the relationship between the expression of PIK3CA, PIK3CB and HPV infection. Methods: The expression of PIK3CA, PIK3CB, p16 and p53 in 156 cases of ESCC was detected by immunohistochemistry. Patients are followed up by telephone or clinic. Results: In this study, the positive rates of PIK3CA, PIK3CB, p53 and p16 in ESCC were significantly higher than in normal esophageal mucosa. The expression of PIK3CA and PIK3CB was related to TNM stage and lymph node metastasis. The expression of p53 is related to the depth of tumor invasion. High p16 expression was found to be significantly correlated with tumor location, TNM stage, differentiation grade and lymph node metastasis. P16 expression is positively correlated with PIK3CA expression but not with PIK3CB and p53. Survival analysis further indicated that that PIK3CA and p53 were markers of poor diagnosis. P16 is a mark of favorable prognosis in ESCC. Conclusions: The expression of PIK3CA and PIK3CB is related to the development of ESCC. The imbalance of PI3K/Akt signaling pathway is closely related to HPV infection and prognosis. The combined detection of PIK3CA and p16 is of great significance to evaluate the prognosis and optimize the treatment of ESCC.
The role of Vitamin D in the prevention of Coronavirus Disease 2019 infection and mortality
Authors: Ilie Petre Cristian , Stefanescu Simina , Smith Lee 
Published: April 2020

Abstract
Background/Aims: WHO declared SARS-Cov-2 a global pandemic. The aims of this paper are to assess if there is any association between mean levels of vitamin D in various countries and cases respectively mortality caused by COVID-19.Methods: We have identified the mean levels of vitamin D for 20 Europeans Countries for which we have also got the data regarding the morbidity and mortality caused by COVID-19.Results: The mean level of vitamin D (average 56mmol/L, STDEV 10.61) in each country was strongly associated with the number of cases/1M (mean 295.95, STDEV 298.73 p=0.004, respectively with the mortality/1M (mean 5.96, STDEV 15.13, p < 0.00001).Discussion: Vitamin D levels are severely low in the aging population especially in Spain, Italy and Switzerland. This is also the most vulnerable group of population for COVID-19.Conclusions: We believe, that we can advise Vitamin D supplementation to protect against SARS-CoV2 infection.
Photocatalytic properties of BiFeO 3 powders synthesized by mixture of CTAB and Glycine
Authors: Asefi N. , Hasheminiasari Masood , Masoudpanah S. M. 
Published: April 2020

Abstract
Highly pure BiFeO3 (BFO) powders were prepared by solution combustion synthesis method using cetyltrimethylammonium bromide (CTAB) and glycine as fuels at various fuel to oxidant (φ) ratios. Microstructural characteristics, morphology, optical properties, and thermal analysis were studied by X-ray diffraction (XRD), scanning electron microscopy (SEM), diffuse reflectance spectroscopy (DRS), and differential thermal/thermogravimetry (DTA/TGA), respectively. The combusted powders prepared at different fuel content contained some impurity phases such as Bi24Fe2O39 and Bi2Fe4O9. During the calcination of BFO powders at 600 °C for 1 hour, nearly pure BFO phase was produced. About 80% of methylene blue dye was photodegraded by combusted powders at φ=2 through 90 minutes of visible light irradiation.