bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermal stress involved in TRPV2 promotes tumorigenesis through the pathway of PI3K/Akt/mTOR in esophageal squamous cell carcinoma
Authors: Huang Rongqi , Li Shuai , Tian Chao , Zhou Peng , Zhao Huifang , Xie Wei , Xiao Jie , Lin Zuoxian , He Lang , Han Xiaobo , Cheng Na , Tang Feng , Yang Yuchen , Huang Hualin , Li Zhiyuan 
Published: September 2020

Abstract
BackgroundEsophageal squamous cell carcinoma (ESCC) is a leading cause of cancer death worldwide. Although the exposure of esophageal mucosa to heat stimuli has long been recognized as an important risk for the initiation and development of ESCC, its underlying mechanisms remain uncharacterized. MethodsWestern blotting and immunofluorescence were used to detect the expression and localization of transient receptor potential vanilloid receptor 2 (TRPV2) in the ESCC cells. The cancerous behaviors of the ESCC cells were evaluated by a single-cell culturing assay, a wound healing assay, a 3D culturing assay and a tube formation assay respectively. In vivo tumorigenicity and metastasis of the ESCC cells were examined via xenograft nude mouse models. Western blotting and IHC were performed to determine the expression profiles of TRPV2 in the ESCC patient tissues. TRPV2 knocked-out ESCC cell line was established using CRISPR-Cas9 gene editing technique.ResultsHere, we found that the expression of TRPV2, one of the thermally sensitive TRP family members, was upregulated in both ESCC cells and clinical samples. We further showed that activation of TRPV2 by recurrent acute thermal stress (54°C, a temperature unexpectedly much lower than those in many dietary modalities) or O1821 (20 μM), a TRPV2 agonist, promoted cancerous behaviors in ESCC cells. The proangiogenic capacity of the heat-challenged ESCC cells was also found to be enhanced profoundly in the tube formation assay; both tumor formation and metastasis that originated from the cells were substantially promoted in nude mouse models upon the activation of TRPV2. These effects were inhibited significantly by tranilast (120 μM), a TRPV2 inhibitor, and abolished by TRPV2 knock-out using CRISPR-Cas9 gene editing. Conversely, overexpression of TRPV2 by transfection of TRPV2 DNA into NE2 cells, could switch the cells to tumorigenesis upon activation of TRPV2. Mechanistically, the driving role of TRPV2 in the progression of ESCC is mainly regulated by the PI3K/Akt/mTOR signaling pathway. Application of a pan-PI3K/mTOR inhibitor and/or a PTEN activator resulted in markedly reduced ESCC cell proliferation. Conclusions﻿Our study first proved that TRPV2 plays an important role in the tumorigenesis of ESCC upon thermal stress. We revealed that TRPV2-PI3K/Akt/mTOR is a novel and promising target for the prevention and treatment of ESCC.
Crosslinkable poloxamer in-situ gel as a submucosal cushion for endoscopic submucosal dissection
Authors: Gao Yuan , Huang Chao , Chen Jiyuan , Tai Zongguang , Li Yan , Gao Shen , Wan Xinjian 
Published: September 2020

Abstract
Background: Hypertonic saline solution is used for endoscopic submucosal dissection (ESD) as a submucosal fluid cushion (SFC). However, the application of SFC is limited by challenges including a short effect duration as well as increased risk of perforation and bleeding.Methods: An in-situ gel crosslinked acrylate/thiol Poloxamer 407 (PA-PSH) can be used as a novel SFC for ESD to avoid perforation, and the feasibility and safety of the PA-PSH for ESD was evaluated in vitro and in vivo .Results: PA-PSH thermal injection led to a longer-lasting elevation with clearer margins as compared with glycerol, with a high mucosal elevation induced by thiol-ene reaction in physiological conditions between acrylate Poloxamer 407 (PA) and thiol-capped Poloxamer 407 (PSH). Precise ESD along the margins of the elevated mucosa was achieved without obvious adverse effects such as bleeding, perforation and tissue damage.Conclusion: The PA-PSH in-situ gel provides an excellent submucosal injection system, which has great potential to improve the ESD technique.
The Potential Influence of Absence of Blinding Method: Secondary Analysis From Randomized Controlled Trials Concerned Acupuncture for Depression
Authors: Wang Rui-Ting , Cui Ling-Jun , Liu Jian-Ping , Han Mei , Fei Yu-Tong , Cao Huijuan 
Published: September 2020

Abstract
Background To evaluate the possibility and impact of performance and detection bias in randomized controlled trials due to lack of the blinding method. MethodsTrials assessing acupuncture for depression published by March 2020 were searched from China National Knowledge Infrastructure database. Through matching, we reassembled the trials of comparing experimental acupuncture and control acupuncture. Meta-analysis with post-treatment HAMD (Hamilton Depression Scale) of the newly reassembled trials was conducted. Changes of HAMD of all included groups were also analyzed, the between groups difference was then analyzed by t-test. All statistical analyses were performed using RevMan 5.3 software. ResultsThirteen pairs of studies were matched from 63 included trials. Re-analysis from the paired studies showed obvious difference of HAMD scales between the experimental and control acupuncture (Mean Difference was -2.95 for HAMD-17, -5.55 for HAMD-24). Re-analysis from all the included groups also showed the statistical difference between groups at four weeks of HAMD-17 (P=0.01), and four/six weeks of HAMD-24 (P<0.01).Conclusion Effectiveness of acupuncture for decreasing the severity of depression was superior in trials that considered acupuncture as experimental treatment, showing performance and detection bias caused by the absence of blinding method is of great significance in acupuncture trials.
Evaluation of the Efficacy and Tolerability of Dolutegravir Plus Lamivudine as a Switch Strategy in Treatment-experienced HIV-infected Patients
Authors: Hua Wei , Wang Sen , Su Bin , Wang Wenxin , Liu An , Li Zaicun , Zhang Tong , Shao Ying , Ye Jiangzhu , Li Jianwei , Dai Lili , Sun Lijun 
Published: September 2020

Abstract
Background: Dual therapy containing dolutegravir (DTG) plus lamivudine (3TC) is a reasonable alternative antiretroviral therapy (ART) option in treatment-experienced patients, which could prevent long-term toxicity and reduce treatment cost. But so far, the effectiveness as safety data of this two-drug regimen is still limited. Methods: This observational study included treatment-experienced HIV-infected patients who switched to a dual regimen containing DTG (50mg/QD) plus 3TC (300mg/QD). Efficacy (HIV RNA <50 copies/mL), safety, and metabolic changes based on laboratory and clinical findings at 48 weeks were analyzed.Results: A total of 33 patients were included, who switched for the following reasons: treatment failure in 5 /33patients (15.2%), simplification in 10 patients (30.3%), and drug toxicity in 18 patients (54.5%). The patients were treated with ART for a median of 2.1 years (interquartile range = 1.0-4.2) before drug switching, and 28/33(84.8%) patients were virologically suppressed at baseline. After switching to DTG plus 3TC, all 33 patients (100%) showed HIV RNA <50 copies/mL after 48 weeks, and the CD4+ cell count was significantly increased (+82 cell/mm3, p=0.0071). We observed a significant increase in LDL-C (+0.77mg/dL, p<0.0001) after switch, however, triglycerides (-0.6mg/dL, p=0.0179) and cholesterol/HDL-C ratio (-0.327, p=0.0366) were found significantly decreased. A significant decrease in eGFR was also observed (-17.8 ml/min, p=0.0047). No patient discontinued due to adverse events.Conclusions: The use of dual therapy containing DTG and 3TC is effective and well-tolerated in treatment-experienced patients under any prior ART, without significant adverse drug reaction.
Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockade
Authors: Palmer Douglas , Webber Beau , Patel Yogin , Johnson Matthew , Kariya Christine , Lahr Walker , Parkhurst Maria , Gartner Jared , Prickett Todd , Lowery Frank , Kishton Rigel , Gurusamy Devikala , Franco Zulmarie , Vodnala Suman , Diers Miechaleen , Wolf Natalie , Slipek Nicholas , McKenna David , Sumstad Darin , Viney Lydia , Henley Tom , Bürckstümmer Tilmann , Baker Oliver , Hu Ying , Yan Chunhua , Meerzaman Daoud , Padhan Kartik , Moriarity Branden , Lo Winifred , Malekzadeh Parisa , Jia Li , Deniger Drew , Patel Shashank , Robbins Paul , McIvor R. Scott , Choudhry Modassir , Restifo Nicholas , Rosenberg Steven 
Published: September 2020

Abstract
While neoantigen-specific tumor infiltrating lymphocytes (TIL) can be derived from in antigen-expressing tumors, their adoptive transfer fails to consistently elicit durable tumor regression. There has been much focus on the role of activation/exhaustion markers such as PD1, CD39 and TOX in TIL senescence. We found these markers were inversely expressed to Cytokine-Induced SH2 protein (CISH), a negative regulator of TCR signaling and tumor immunity in mice. To evaluate the physiological role of CISH in human TIL we developed a high-efficiency CRIPSR-based method to knock out CISH in fully mature TIL. CISH KO resulted in increased T cell receptor (TCR) avidity, tumor cytolysis and neoantigen recognition. CISH expression in the tumor resections correlated with TIL inactivity against p53 hotspot mutations and CISH KO in TIL unmasked reactivity against these universal neoantigens. While CISH KO resulted in T cell hyperactivation and expansion it did not alter maturation, perhaps by preferential PLCγ-1 and not AKT inhibition. Lastly, CISH KO in T cells increased PD1 expression and the adoptive transfer of Cish KO T cells synergistically combines with PD1 antibody blockade resulting in durable tumor regression and survival in a preclinical animal model. These data offer new insights into the regulation of neoantigen recognition, expression of activation/exhaustion markers, and functional/maturation signals in tumor-specific T cells.
Abdominopelvic leiomyoma with large ascites: a case report and literature review
Authors: Wang Yiwei , Fan Qiong , Qian Zhaoxia , Wang Jinjin , Li Yuhong , Wang Yudong 
Published: September 2020

Abstract
BackgroundLeiomyoma of the uterus is relatively common, but uterine leiomyoma of the greater omentum is rare. Case presentationHere, we report a case of a 22-year-old woman who presented with a 3-month history of progressive abdominal distension and a hypervascular abdominopelvic mass. Due to a high serum concentration of CA125, the preoperative diagnosis was unclear. During surgery, 5 L of ascites was removed. An 18.8-cm solid mass, which was pedunculated from the uterine fundus and exhibited complex adhesion to the greater omentum, was removed. The CA125 level was reduced postoperatively, and a pathologic study confirmed that the mass was a leiomyoma that originated in the uterus.ConclusionUterine leiomyoma can share vessels with the greater omentum. This case highlights the difficulty of diagnosing pseudo-Meigs syndrome and the importance of imaging and laboratory examinations.
A Combination of UPLC-LTQ-Orbitrap-MSn Research, Network Analysis and Experimental Verification Used to Explore the Mechanism of Yangyin Yiqi Huoxue Recipe in Treating Sjogren’s Syndrome
Authors: Lu Wen-Wen , Fu Tian-Xiao , Wu Fang-Ping , Xiong Fu-lin , Li Tian-Yi , Wu Guo-lin 
Published: September 2020

Abstract
Purpose: To explore the main active ingredients and mechanism of Yangyin Yiqi Huoxue (YYYQHX) Recipe in treating Sjogren’s syndrome.Methods: The main active ingredients of Yangyin Yiqi Huoxue(YYYQHX) Recipe were obtained through UPLC-LTQ-Orbitrap-MSn research technical analysis. Subsequently, the main active ingredients were analyzed by network pharmacology to explore their important targets and biological processes. Finally, the important target M3 muscarinic acetylcholine receptor (CHRM3) gene was verified by cytological experiments.Results: We identified 41 active ingredients in the YYYQHX Recipe, and identified 26 potential targets through network pharmacology methods. According to the network analysis and literature research, CHRM3 is one of the main targets of YYYQHX in the treatment of Sjogren’s syndrome. We observed the effect of YYYQHX on the CHRM3 and aquaporin 5(AQP5) genes using cytological experiments. The results showed that YYYQHX significantly increased the expression of CHRM3 and AQP5 in submandibular gland cells. This may be the mechanism of YYYQHX Recipe in improving symptoms such as dry mouth in Sjogren’s syndrome.Conclusions: This study provides a strategy for understanding the mechanism of action of YYYQHX in treating Sjogren’s syndrome by combining UPLC-LTQ-Orbitrap-MS research, network pharmacology, and experimental validation.
Echo-sonographic semiotics of abdominal tuberculosis
Authors: Plotkin D. V. , Loshkareva E. O. , Kirillova O. V. , Reshetnikov M. N. , Sinitsyn M. V. , Chauzov A. Yu. 
Published: September 2020

The objective of the study: to clarify the echo-sonographic semiotics of abdominal tuberculosis.Subjects and methods. 107 patients at the age from 22 to 58 years old had a sonographic examination and underwent the consequent surgery due to suspected abdominal tuberculosis. 92/107 (85.9%) patients had pulmonary tuberculosis of different severity; 73.9% (68/107) patients pulmonary tuberculosis with concurrent HIV infection.Results: all patients had ultrasound examinations due to pain abdominal syndrome or clinical signs of ascites. The following echo signs were visualized in patients: enlargement and changes in the structure of intra-abdominal lymph nodes (in 58 patients), changes in the intestinal wall (in 19 patients), heterogeneity of serous membranes and free fluid in the abdominal cavity (in 81 patients). In the absolute majority of patients, there was a combination of those echo signs. Among 107 patients with suspected abdominal tuberculosis, this diagnosis was confirmed in 44 (41.1%), while in 63 (58.9%) patients, non-tuberculosis abdominal lesions were detected: 11 – mycobacteriosis, 5 – lymphopropoliferative disease (lymphoma), and 1 – metastatic lesion of lymph nodes (adenocarcinoma), 3 – colorectal cancer (adenocarcinoma) and in 1 (5.3%) – granulomatous colitis (Crohn disease), 4 – peritoneal carcinomatosis, 1– peritoneal sarcoidosis, 1– peritoneal mycobacteriosis (MAC-infection), in 36 patients – free fluid in the abdominal cavity corresponded to secondary peritonitis caused by perforated tuberculosis bowel ulcers or non-specific ascites against the background of hypoproteinemia or drug-induced hepatitis.
Tourmaline composition and boron isotope signature as a tracer of magmatic-hydrothermal processes
Authors: Qiu Kun-Feng , Yu Hao-Cheng , Hetherington Callum , Huang Ya-Qi , Yang Tao , Deng Jun 
Published: September 2020

Abstract
This study presents a petrogeochemical and boron isotope study on tourmaline from the barren Damai, and the contemporaneous but ore-bearing Dewulu and Meiwu intrusions, to better understand the origins, sources, and fluid evolution of magmatic-hydrothermal ore systems and provide ore formation implications for gold, copper, and iron deposits in the Xiahe-Hezuo polymetallic district in the West Qinling, China. Tourmaline from all three intrusions shows similar compositions and encompasses Na-Fe schorl and Na-Mg dravite. Tourmaline at Dewulu is primarily found in tuffaceous breccias and a quartz diorite porphyry. In the tuffaceous breccia body, tourmaline occurs as fine-grained anhedral masses that fill voids and cement fragments of breccia and sickle quartz. Tourmalines in breccia are texturally similar to those formed in typical breccia pipes, which are attributed to explosion or collapse induced by a transition from magmatic to hydrothermal Si- and B-rich fluids. They display element substitutions controlled by Fe2+Mg–1, indicating a reduced environment. Values of δ11B are –6.6 to –4.0‰, representing the primary magmatic-hydrothermal fluids. Tourmaline from the Dewulu quartz diorite porphyry is coarse-grained, euhedral, and found in quartz-sulfide veins. The tourmaline displays oscillatory zoning textures but lacks correlative variations of major elements. The Fe2+Mg–1 and Fe3+Al–1 substitution mechanisms are dominant, demonstrating more oxidized fluids. The δ11B values in the cores, ranging from –7.1 to –5.6‰, suggest that the tourmalines in the quartz veins were inherited from magmatic-hydrothermal fluids that precipitated the fine-grained tourmaline in the tuffaceous breccia body. A large δ11B isotopic fractionation that decreases from cores (–5.6‰) to rims (–10.7‰) indicates significant fractionation during degassing occurred, increasing oxygen fugacity of the residual liquid. The Meiwu locality hosts fine-grained euhedral tourmalines coexisting with magnetite. Their composition is controlled by substitution between Al3+ by Fe3+ and has the lightest δ11B values ranging from –11.4 to –10.0‰. They are interpreted to result from skarn formation under oxidized conditions. In contrast, X☐Al(NaMg)–1 is the dominant substitution mechanism for Damai tourmalines and attributed to (geochemically) reduced fluids with a low salinity. We conclude that tourmalines with low Fe values, substitution mechanisms dominated by Fe3+Al–1, and large shifts of B isotopic composition are potentially an ore-forming indicator in the Xiahe-Hezuo polymetallic district.
The Effect of Hybrid Fibers Reinforcement on the Mechanical and Physical Properties of Concrete
Authors: Khayoun Zaher I. , Kamal Hamza M. , Ibrahim Yasir K. 
Published: September 2020

Background:
Hybrid fiber reinforced concrete is the use of two or more than two fiber textures in a single concrete matrix to improve the overall properties of concrete.

Materials and Methods:
In this study, the ductility of medium strength concrete was observed to improve by incorporating steel and polypropylene in a ratio of 50:50 as a hybrid fiber. Four proportions of Volume fractions (Vf) were used (0.25, 0.5, 0.75, 1%). The changes in the mechanical properties and density occurred due to the addition of these hybrid fibers.

Results:
The features examined include bulk density, compressive strength, and flexural strength by using thirteen concrete mixtures with various volume fractions of steel and polypropylene fibers.

Discussion:
The results showed a significant increase in compressive and flexural strength due to the addition of steel fibers. On the other hand, polypropylene fibers exhibited minor changes in the mechanical properties of hardened concrete exclusively in the mixtures made with both steel and polypropylene fibers in which the strength increased in comparison to plain concrete by 4.4% and to steel fiber group having the optimal strength and other properties.

Conclusion:
These remarks provide a clear view of the importance of using different fiber reinforcing systems to improve the mechanical performance of concrete, which is considered quasi-brittle.
Wearable Health Devices in Health Care: Narrative Systematic Review
Authors: Lu Lin , Zhang Jiayao , Xie Yi , Gao Fei , Xu Song , Wu Xinghuo , Ye Zhewei 
Published: September 2020

Background
With the rise of mobile medicine, the development of new technologies such as smart sensing, and the popularization of personalized health concepts, the field of smart wearable devices has developed rapidly in recent years. Among them, medical wearable devices have become one of the most promising fields. These intelligent devices not only assist people in pursuing a healthier lifestyle but also provide a constant stream of health care data for disease diagnosis and treatment by actively recording physiological parameters and tracking metabolic status. Therefore, wearable medical devices have the potential to become a mainstay of the future mobile medical market.

Objective
Although previous reviews have discussed consumer trends in wearable electronics and the application of wearable technology in recreational and sporting activities, data on broad clinical usefulness are lacking. We aimed to review the current application of wearable devices in health care while highlighting shortcomings for further research. In addition to daily health and safety monitoring, the focus of our work was mainly on the use of wearable devices in clinical practice.

Methods
We conducted a narrative review of the use of wearable devices in health care settings by searching papers in PubMed, EMBASE, Scopus, and the Cochrane Library published since October 2015. Potentially relevant papers were then compared to determine their relevance and reviewed independently for inclusion.

Results
A total of 82 relevant papers drawn from 960 papers on the subject of wearable devices in health care settings were qualitatively analyzed, and the information was synthesized. Our review shows that the wearable medical devices developed so far have been designed for use on all parts of the human body, including the head, limbs, and torso. These devices can be classified into 4 application areas: (1) health and safety monitoring, (2) chronic disease management, (3) disease diagnosis and treatment, and (4) rehabilitation. However, the wearable medical device industry currently faces several important limitations that prevent further use of wearable technology in medical practice, such as difficulties in achieving user-friendly solutions, security and privacy concerns, the lack of industry standards, and various technical bottlenecks.

Conclusions
We predict that with the development of science and technology and the popularization of personalized health concepts, wearable devices will play a greater role in the field of health care and become better integrated into people’s daily lives. However, more research is needed to explore further applications of wearable devices in the medical field. We hope that this review can provide a useful reference for the development of wearable medical devices.