bluuebunny/crossref_metadata_2025_split huggingface dataset:
Commercial experience with miscanthus crops: Establishment, yields and environmental observations
Authors: Shepherd Anita , Clifton‐Brown John , Kam Jason , Buckby Sam , Hastings Astley 
Published: April 2020

AbstractThis study investigates the condition of commercial miscanthus fields, growers’ concerns and reasons for growing the crop and also the modelling of a realistic commercial yield. Juvenile and mature Miscanthus × giganteus crops of varying age are surveyed in growers’ fields across mid‐England. We record in‐field plant density counts and the morphology of crops of different ages. Mature crops thrive on both clay and sandy soils. Plants surveyed appear robust to drought, weeds and disease, the only vulnerability is rhizome condition when planting. Mature miscanthus planted pre‐2014 continues to develop, spreading into planting gaps and growing more tillers. In stands planted post‐2014, improved planting techniques reduce planting gaps and create a reasonably consistent planting density of 12,500 plants/ha. The main reason for growers' investment in miscanthus is not financial return, but relates to its low requirement for field operations, low maintenance cost and regeneration. This offers practical solutions for difficult field access and social acceptability near public places (related to spray operations and crop vandalism). Wildlife is abundant in these fields, largely undisturbed except for harvest. This contributes to the greening of agriculture; fields are also used for gamebird cover and educational tours. This crop is solving practical problems for growers while improving the environment. Observed yield data indicate gradual yield increase with crop age, a yield plateau but no yield decrease since 2006. In stands with low planting densities, yields plateau after 9 years. Surveyed yield data are used to parameterize the MiscanFor bioenergy model. This produces options to simulate either juvenile yields or a yield for a landscape containing different aged crops. For mature English crop yields of 12 t ha−1 year−1, second‐ and third‐year juvenile harvests average 7 t ha−1 year−1 and a surrounding 10 km by 10 km area of distributed crop age would average 9 t ha−1 year−1.
Interleukin‐33 alleviates psoriatic inflammation by suppressing the T helper type 17 immune response
Authors: Chen Zeyu , Hu Yifan , Gong Yu , Zhang Xilin , Cui Lian , Chen Rongfen , Yu Yingyuan , Yu Qian , Chen Youdong , Diao Hongyue , Chen Jia , Wang Yuanyuan , Shi Yuling 
Published: April 2020

SummaryPsoriasis is a chronic inflammatory skin disease with unclear pathogenesis. Interleukin‐33 (IL‐33) is highly expressed in patients with psoriasis, but its role in psoriasis is unknown. The aim of this study was to investigate the possible role of IL‐33 in the pathogenesis and treatment of psoriasis. IL‐33 expression was determined using enzyme‐linked immunosorbent assay, real‐time fluorescent quantitative polymerase chain reaction and immunohistochemical staining. CD4+ T cells were sorted using magnetic beads and treated with or without IL‐33. Imiquimod (IMQ) was used to induce psoriatic inflammation in mice. The frequency of immune cells was determined using flow cytometry. The cytokine level in mouse skin was measured using cytometric bead array. Our results showed that IL‐33 was highly expressed in the lesional skin and serum of patients with moderate‐to‐severe plaque psoriasis. IL‐33 inhibited the expression of IL‐17 in CD4+ T cells of psoriasis patients. Subcutaneous injection of IL‐33 alleviated the IMQ‐induced psoriatic inflammation in mice, reduced tumor necrosis factor‐α and IL‐23 expression, and decreased the proportion of T helper type 17 (Th17) cells in the skin‐draining lymph nodes in the mice. Our results suggest that IL‐33 plays a protective role in the pathogenesis of psoriasis by suppressing Th17 cell differentiation and function. The potential therapeutic effect of IL‐33 in treating psoriasis warrants further investigation.
A five‐mRNA signature associated with post‐translational modifications can better predict recurrence and survival in cervical cancer
Authors: Ju Mingyi , Qi Aoshuang , Bi Jia , Zhao Lan , Jiang Longyang , Zhang Qiang , Wei Qian , Guan Qiutong , Li Xueping , Wang Lin , Wei Minjie , Zhao Lin 
Published: April 2020

AbstractHigh mortality of patients with cervical cancer (CC) stresses the imperative of prognostic biomarkers for CC patients. Additionally, the vital status of post‐translational modifications (PTMs) in the progression of cancers has been reported by numerous researches. Therefore, the purpose of this research was to dig a prognostic signature correlated with PTMs for CC. We built a five‐mRNA (GALNTL6, ARSE, DPAGT1, GANAB and FURIN) prognostic signature associated with PTMs to predict both disease‐free survival (DFS) (hazard ratio [HR] = 3.967, 95% CI = 1.985‐7.927; P < .001) and overall survival (HR = 2.092, 95% CI = 1.138‐3.847; P = .018) for CC using data from The Cancer Genome Atlas database. Then, the robustness of the signature was validated using GSE44001 and the Human Protein Atlas (HPA) database. CIBERSORT algorithm analysis displayed that activated CD4 memory T cell was also an independent indicator for DFS (HR = 0.426, 95% CI = 0.186‐0.978; P = .044) which could add additional prognostic value to the signature. Collectively, the PTM‐related signature and activated CD4 memory T cell can provide new avenues for the prognostic predication of CC. These findings give further insights into effective treatment strategies for CC, providing opportunities for further experimental and clinical validations.
Field monitoring of onion maggot (Delia antiqua) fly through improved trapping
Authors: Willett Denis S. , Filgueiras Camila C. , Nyrop Jan P. , Nault Brian A. 
Published: April 2020

AbstractOnion maggot (Delia antiqua) is an economically important pest of Allium crops in temperate regions throughout the world. Management of this pest is necessary to achieve economic returns and depends on insecticide regimes and cultural management. Current cultural management especially altering planting date, field location and crop rotation depend on monitoring. We evaluated the effect of shape, size, colour and chemical attractants on trap catch of field populations of adult D. antiqua flies in upstate New York. White, large diameter, spherical traps in conjunction with Delia Lure attractant performed the best in attracting and catching D. antiqua adults. These results suggest an improved means of attracting and capturing D. antiqua populations which could be useful in monitoring efforts and development of attract and kill strategies for pest control.
Friend or enemy? Review of 17β‐HSD10 and its role in human health or disease
Authors: Vinklarova Lucie , Schmidt Monika , Benek Ondrej , Kuca Kamil , Gunn‐Moore Frank , Musilek Kamil 
Published: April 2020

Abstract17β‐hydroxysteroid dehydrogenase (17β‐HSD10) is a multifunctional human enzyme with important roles both as a structural component and also as a catalyst of many metabolic pathways. This mitochondrial enzyme has important functions in the metabolism, development and aging of the neural system, where it is involved in the homeostasis of neurosteroids, especially in regard to estradiol, changes in which make it an essential part of neurodegenerative pathology. These roles therefore, indicate that 17β‐HSD10 may be a possible druggable target for neurodegenerative diseases including Alzheimer's disease (AD), and in hormone‐dependent cancer. The objective of this review was to provide a summary about physiological functions and pathological roles of 17β‐HSD10 and the modulators of its activity.
image
Faecal pH throughout the reproductive cycle of sows in commercial pig herds
Authors: Maes Dominiek , Chantziaras Ilias , Vallaey Eline , Demeyere Kristel , Meyer Evelyne , Janssens Geert P. J. 
Published: April 2020

AbstractDespite the importance of swine intestinal health, there are no easy‐to‐use and cost‐efficient methods to evaluate it under field conditions for sows. To provide some indication about intestinal health, pH of faeces could be used but reference values for the currently high‐performing commercial sow breeds are missing. In response, faecal pH of healthy sows from three different herds (herd A: 230 sows, herd B: 350 sows, herd C: 500 sows) was measured throughout the reproductive cycle. Within each herd, 10 sows were selected and rectal faeces samples were collected at different time points during the reproductive cycle: day 90 of gestation, day 1, 7, 14 and 21 of lactation, 7 days post‐weaning and day 30 of the next gestation. In addition, data on sow body condition (back fat), feed composition and coarseness of the feed were collected. For individual pH measurements, the pH ranged from 6.30 to 7.93. However, for all herds together, the average faecal pH value of the sows throughout the reproductive cycle ranged from 6.89 to 7.15. Also, the variations due to sow and time of sampling during the reproductive cycle were low with coefficients of variation of less than 5%. The results from the pairwise comparisons of the statistical model showed that in the last stages of lactation (i.e., at day 21), significantly lower average pH values (p ≤ .05) are expected when compared to earlier stages of lactation (days 3 or 7), or at day 7 post‐weaning or compared to day 30 of the next gestation. Bearing its limitations, our study provided reference faecal pH values from high‐performing commercial sows under field conditions and as such they could be used directly in the field. Yet, further research is needed to provide more information on the factors affecting pH values throughout the reproductive cycle of the sow.
Optimization of medium using response surface methodology to enhance the growth of Effrenium voratum (Symbiodiniaceae, Dinophyceae)
Authors: Yang Fangfang , Long Chao , Wei Zhangliang , Long Lijuan 
Published: April 2020

Survival of coral reef‐associated Symbiodiniaceae is vital to maintain the healthy coral community in coral reefs. However, knowledge about cultivation of free‐living or symbiotic Symbiodiniaceae has been limited. In this study, the response surface methodology was applied to optimize the medium for Effrenium voratum. The results showed that the impacts of nutrient components on algal growth were: FeCl3> NaH2PO4>MnSO4> MgSO4/CoSO4 > KCl>ZnSO4> CaCl2/NaNO3, among which NaH2PO4 and FeCl3 significantly affected algal growth. The optimal medium was: natural seawater supplemented with NaH2PO4·2H2O 0.25 mM,FeCl3·6H2O 14.24 μM, NaNO3 0.94 mM, MgSO4·7H2O 40.63 mM, KCl 5.37 mM, CaCl2·2H2O 4.08 mM, ZnSO4·7H2O 0.35 μM, MnSO4 9.93 μM, and CoSO4 0.36 μM. The use of the optimized medium resulted in an increase of biomass yield (0.76 g dry weight · L−1) by 46% over that using the initial medium, which agreed with the predicted value (0.71 g · L−1). Additionally, fatty acids, mainly consisting of palmitic acid (C16:0) and ethyl carbonate (C20:0), accounted for approximately 50% of the total fatty acids in E. voratum. Interestingly, docosahexaenoic acid (DHA) accounted for 6% of total fatty acids, a high proportion that makes E. voratum a potential candidate feedstock in aquaculture for DHA production.
Dimethyl fumarate ameliorates hepatic inflammation in alcohol related liver disease
Authors: Sangineto Moris , Grabherr Felix , Adolph Timon E. , Grander Christoph , Reider Simon , Jaschke Nikolai , Mayr Lisa , Schwärzler Julian , Dallio Marcello , Moschen Alexander R. , Moschetta Antonio , Sabbà Carlo , Tilg Herbert 
Published: April 2020

AbstractBackground & AimsAlcohol‐related liver disease (ALD) comprises different liver disorders which impose a health care issue. ALD and particularly alcoholic steatohepatitis, an acute inflammatory condition, cause a substantial morbidity and mortality as effective treatment options remain elusive. Inflammation in ALD is fuelled by macrophages (Kupffer cells [KCs]) which are activated by intestinal pathogen associated molecular patterns, eg lipopolysaccharide (LPS), disseminated beyond a defective intestinal barrier. We hypothesized that the immunomodulator dimethyl‐fumarate (DMF), which is approved for the treatment of human inflammatory conditions such as multiple sclerosis or psoriasis, ameliorates the course of experimental ALD.MethodsDimethyl‐fumarate or vehicle was orally administered to wild‐type mice receiving a Lieber‐DeCarli diet containing 5% ethanol for 15 days. Liver injury, steatosis and inflammation were evaluated by histology, biochemical‐ and immunoassays. Moreover, we investigated a direct immunosuppressive effect of DMF on KCs and explored a potential impact on ethanol‐induced intestinal barrier disruption.ResultsDimethyl‐fumarate protected against ethanol‐induced hepatic injury, steatosis and inflammation in mice. Specifically, we observed reduced hepatic triglyceride and ALT accumulation, reduced hepatic expression of inflammatory cytokines (Tnf‐α, Il‐1β, Cxcl1) and reduced abundance of neutrophils and macrophages in ethanol‐fed and DMF‐treated mice when compared to vehicle. DMF protected against ethanol‐induced barrier disruption and abrogated systemic LPS concentration. In addition, DMF abolished LPS‐induced cytokine responses of KCs.ConclusionsDimethyl‐fumarate counteracts ethanol‐induced barrier dysfunction, suppresses inflammatory responses of KCs and ameliorates hepatic inflammation and steatosis, hallmarks of experimental ALD. Our data indicates that DMF treatment might be beneficial in human ALD and respective clinical trials are eagerly awaited.
Cross‐species transmission and evolutionary dynamics of canine distemper virus during a spillover in African lions of Serengeti National Park
Authors: Weckworth Julie K. , Davis Brian W. , Dubovi Edward , Fountain‐Jones Nicholas , Packer Craig , Cleaveland Sarah , Craft Meggan E. , Eblate Ernest , Schwartz Michael , Mills L. Scott , Roelke‐Parker Melody 
Published: April 2020

AbstractThe outcome of pathogen spillover from a reservoir to a novel host population can range from a “dead‐end” when there is no onward transmission in the recipient population, to epidemic spread and even establishment in new hosts. Understanding the evolutionary epidemiology of spillover events leading to discrete outcomes in novel hosts is key to predicting risk and can lead to a better understanding of the mechanisms of emergence. Here we use a Bayesian phylodynamic approach to examine cross‐species transmission and evolutionary dynamics during a canine distemper virus (CDV) spillover event causing clinical disease and population decline in an African lion population (Panthera leo) in the Serengeti Ecological Region between 1993 and 1994. Using 21 near‐complete viral genomes from four species we found that this large‐scale outbreak was likely ignited by a single cross‐species spillover event from a canid reservoir to noncanid hosts <1 year before disease detection and explosive spread of CDV in lions. Cross‐species transmission from other noncanid species probably fuelled the high prevalence of CDV across spatially structured lion prides. Multiple lines of evidence suggest that spotted hyenas (Crocuta crocuta) could have acted as the proximate source of CDV exposure in lions. We report 13 nucleotide substitutions segregating CDV strains found in canids and noncanids. Our results are consistent with the hypothesis that virus evolution played a role in CDV emergence in noncanid hosts following spillover during the outbreak, suggest that host barriers to clinical infection can limit outcomes of CDV spillover in novel host species.
Demography and adaptation promoting evolutionary transitions in a mammalian genus that diversified during the Pleistocene
Authors: de Jong Menno J. , Li Zhipeng , Qin Yanli , Quéméré Erwan , Baker Karis , Wang Wen , Hoelzel A. Rus 
Published: April 2020

AbstractSpecies that evolved in temperate regions during the Pleistocene experienced periods of extreme climatic transitions. Consequent population fragmentation and dynamics had the potential to generate small, isolated populations where the influence of genetic drift would be expected to be strong. We use comparative genomics to assess the evolutionary influence of historical demographics and natural selection through a series of transitions associated with the formation of the genus Capreolus, speciation within this genus during the Quaternary and during divergence among European roe deer (C. capreolus) populations. Our analyses were facilitated by the generation of a new high‐coverage reference genome for the Siberian roe deer (C. pygargus). We find progressive reductions in effective population size (Ne), despite very large census sizes in modern C. capreolus populations and show that low Ne has impacted the C. capreolus genome, reducing diversity and increasing linkage disequilibrium. Even so, we find evidence for natural selection shared among C. capreolus populations, including a historically documented founder population that has been through a severe bottleneck. During each phylogenetic transition there is evidence for selection (from dN/dS and nucleotide diversity tests), including at loci associated with diapause (delayed embryonic development), a phenotype restricted to this genus among the even‐toed ungulates. Together these data allow us to assess expectations for the origin and diversification of a mammalian genus during a period of extreme environmental change.
Implementing, embedding and sustaining simulation‐based education: What helps, what hinders
Authors: Ferguson Jane , Astbury Jayne , Willis Sarah , Silverthorne Jennifer , Schafheutle Ellen 
Published: April 2020

AbstractObjectivesAlthough there is much evidence to support the use of simulation‐based education (SBE) in undergraduate education of health care professionals, less attention has been paid to how SBE, viewed as a complex intervention, is implemented and becomes embedded and sustained. This paper aims to explore factors that inhibited or promoted SBE becoming normal practice in undergraduate health care professional programmes.MethodsParticipants involved in the organisation, design and delivery of SBE in the north of England were recruited purposefully from higher education institutions (HEI) and National Health Service (NHS) Trusts through local networks for qualitative telephone interviews. Transcripts were analysed inductively using a hybrid approach involving simultaneous inductive open coding and deductive coding using normalisation process theory (NPT) as a theoretical lens.FindingsA total of 12 NHS staff from 11 trusts and seven individuals from four HEIs were interviewed. There was considerable variation in the approach taken to implementation across organisations, which resulted in varying degrees of embeddedness. Implementation was challenged or enabled by organisational leadership, professional buy‐in and the development and maturity of the strategic approach. Variation in understanding of the scope and pedagogical aims of SBE led to inequity between professions and organisations in investment and participation, as well as design and delivery of SBE.ConclusionsGiven the complexity of SBE, best practice in implementation should be considered fundamental to the successful delivery of SBE. The findings provide an explanation of how contextual factors can support or hinder implementation to maximise potential benefits and learning outcomes; this understanding can be used to better inform development of SBE strategies and highlight potential factors needed to navigate contextual barriers so that learning outcomes can be maximised.
Retrieval of lingually displaced root of lower third molar report of two cases and review of literature
Authors: Dhanrajani Parmanand 
Published: April 2020

AbstractAccidental displacement of teeth or roots can occur during extractions. Lingually inclined or deeply impacted mandibular third molar teeth may have a higher risk of being displaced into soft tissues. A case of lingually displaced root while removing lower third molar is described. The techniques used to minimise the risk of accidental displacement of teeth and roots during extraction are discussed. The importance of recognising this complication and methods of retrieval are highlighted.
Pediatric anesthetic implications of COVID‐19—A review of current literature
Authors: Lee‐Archer Paul , von Ungern‐Sternberg Britta S. 
Published: April 2020

AbstractPediatric anesthetists have an important role to play in the management of patients suspected or confirmed to have COVID‐19. In many institutions, the COVID‐19 intubation teams are staffed with anesthetists as the proceduralists working throughout the hospitals also in the ICU and Emergency Departments. As practitioners who perform aerosol generating procedures involving the airway, we are at high risk of exposure to the virus SARS‐CoV‐2 and need to ensure we are well prepared and trained to manage such cases. This article reviews the relevant pediatric literature surrounding COVID‐19 and summarizes the key recommendations for anesthetists involved in the care of children during this pandemic.
Reduction in Migraine and Headache Frequency and Intensity With Combined Antioxidant Prophylaxis (N‐acetylcysteine, Vitamin E, and Vitamin C): A Randomized Sham‐Controlled Pilot Study
Authors: Visser Eric John , Drummond Peter D. , Lee‐Visser Julia L. A. 
Published: April 2020

AbstractObjectiveTo investigate the preventive effects of a combined antioxidant drug (N‐acetylcysteine, vitamin E, and vitamin C [NEC]) on migraine outcomes. Migraine is characterized by increased oxidative stress and neurogenic inflammation in the brain; therefore, antioxidants may have a migraine preventive effect.DesignRandomized, double‐blind, sham‐controlled pilot study. SettingAustralian community.SubjectsAdults reporting 2 to 8 migraines per month for at least a year.MethodsAfter a 1‐month baseline period, 35 subjects completed 3 months of treatment with NEC (n = 19) or sham (n = 16) capsules. The primary outcome was the difference in mean number of headaches per month between baseline and final month of the trial for NEC and sham groups; secondary outcomes are listed below.ResultsFor NEC there was a significant decrease in mean number of headaches by 3.0 per month (P = 0.004) compared with 1.4 for sham (P = 0.073); there was no significant difference in these changes between the 2 groups (P = 0.052). Average monthly headache (P = 0.041) and migraine frequency (P = 0.018) were significantly less for NEC vs. sham. In NEC subjects, there was a significant decrease in average monthly migraine days (−3.1), moderate/severe headache days (−3.2), migraine duration, headache pain scores, and acute headache medication use.ConclusionsThis is the first randomized controlled trial to find that combined antioxidant therapy with NEC reduces headaches and migraines in adult migraineurs. Given the limitations of this pilot study, an adequately powered randomized controlled trial is planned to further investigate antioxidant prophylaxis in migraine.