bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transaction records as a tool for bird trade monitoring in supporting bird’s conservation and sustainable use
Authors: Irham M , Yohanna  , Kurniawan P T 
Published: March 2020

Abstract
Birds market survey has been proofed to be effective as a complementary method to evaluate the population of birds in the wild. We implemented this method to monitor the birds trade in Jambi. Rather than direct observation to the market, we examine the transaction records from three wholesalers to obtain data. Our results show that during the period of January-September 2017 the total birds traded is 11,660 specimens and transaction value is Rp 941,932,900.00. Number of generic species is 76 in which some names refer to same species. From those generic species, we can identify about 26 species and the rest of birds is grouped according to their genus or family by which consist of 11 kind of birds. There are 4 species (and group of species) that traded over 1000 specimens i.e Acridotheres javanicus (2,824), Chloropsis sp. (2,185), Garrulax mitratus (1,266), and Zosterops sp. (1,355). The expensive songbirds such as Pycnonotus zeylanicus and Kittacincla malabarica are absent but the least expensive species but favorable, Copsychus saularis, is only traded in small number (157 specimens). Combine field survey and market survey may give better picture of overall bird population that critical for setting up conversation strategy of Indonesian birds.
Genetic analysis and molecular phylogeny of zigzag leafhopper Maiestas dorsalis (Motschulsky) using mitochondrial COI gene
Authors: Manurung B , Hasairin A , Daulae A H 
Published: March 2020

Abstract
The zigzag leafhopper Maiestas dorsalis (Hemiptera) is a tungro virus vector that cause damage on rice plants. Genetic analysis of M. dorsalis that be isolated from Samosir island, North Sumatra, Indonesia using partial DNA sequence of mitochondrial cytochrome oxidase subunit I (COI) DNA is still limited. This study aims to identify and to find out the genetic data of M. dorsalis that be derived from region of its mitochondrial cytochrome oxidase I gene (COI). Analysis was done by four steps, namely: Hopper collection on paddy field by using insect net, DNA extraction by using Zymo Tissue & Insect DNA Mini Preparation, amplification by PCR using My TaqTM HS Red Mix and DNA sequence analysis using ABI PRISM 3730xl Genetic Analyzer. Primer cocktail tRWF-Mlep was used in DNA amplification step. The research result pointed out that COI DNA fragment of M. dorsalis has length 521 bp. This COI DNA sequence was dominated by A and T(U) bases with concentration 74.30%. The concentration of T(U), C, A and G nucleotides in the COI sequence were 35.90%, 13.40%, 38.44%, and 12.30%, respectively. Identification of M. dorsalis based on this COI DNA sequence confirmed the identification result based on its morphological characters.
Revisiting diversity of coccinellid beetle in Mount Gede-Pangrango National Park: a preliminary observation
Authors: Maulana A , Wardaningsih A S , Widayanti M , Prawasti T S 
Published: March 2020

Abstract
Declines in insect population and diversity particularly in the tropical forest region have stimulated wide interest among researchers worldwide. Loss of insect diversity and abundance is expected to provoke cascading effect on the ecosystem, but few data and documentation are available. So far, the coccinellid beetle study in Mount Gede-Pangrango National Park, Indonesia was conducted more than 40 years ago with last well-documented exploration by 2004. We aimed to update and deliver the newest data on the diversity of coccinellids of Mount Gede-Pangrango National Park. We tracked along the interpretation path and beetles were collected using hand method during observation between July 2018 to October 2019. A total of 17 species coccinellids were identified. Seven species belong to the phytophagous group of Diekeana, Epilachna, and Henosepilachna. Two mycophagous species from Psylloborini and Sticholotidini tribe, while others species (8 species) are a member of the predatory group, including Coccinellini and Chilocorini tribe. In this study, plenty of them are common species and widely distributed in the open area of the buffer zone. Our temporary findings indicate the possibility of species loss compared to past surveys that discover rare taxa, such as Ryszardia, and other specialist Epilachna group infesting wild host-plants.
Contradictory between morphology and phylogenetic trees of Orthosiphon spp. (Lamiaceae) from Indonesia
Authors: Sudarmono  , Kim S Y , Paik J H 
Published: March 2020

Abstract
The nuclear ribosomal Internal Transcribed Spacer region (ITS), and three chloroplast loci (trnL-trnF, rps16 and trnL) were also carried out in this study. Morphological analysis of leaves, stems, and flowers is used to identify visual differences. This study was the purpose of analyzed morphological and phylogenetic relationships among ten taxa of Orthosiphon from the analysis of DNA sequences. The results that the difference in leaves, flowers and stems is very striking in seven taxa of Orthosiphon aristatus (OGP and OGW) and O. endanghidayatae (OWW, OG + W and OW + W). The phylogenetic trees constructed from cpDNA (trnL gene, trnL-trnF intergenic spacer region, and rps16 region) of using NJ methods among taxa of Orthosiphon aristatus and O. endanghidayatae collected from Indonesia showed constant topologies with high bootstrap values (BS 86% and 100%, respectively), but were different from those ITS of nrDNA that the phylogenetic trees supported low bootstrap values (65% by NJ). Although differing from morphology to species of Orthosiphon spp. but still similar in DNA analysis. The results of this study indicate that the speciation process of O. endanghidayatae (OWW, OG + W and OW + W) is as a process of morphological changes faster than the process of genetic change.
Diversity of dragonflies (Insecta: Odonata) in Ujung Kulon National Park
Authors: Sugiman U , Atmowidi T , Priawandiputra W 
Published: March 2020

Abstract
Ujung Kulon National Park is a lowland tropical forest that was still preserved. There was limited information about the diversity of dragonflies in Ujung Kulon National Park. The purpose of this study was to study species diversity of dragonfly in Ujung Kulon. The research was conducted in Ujung Kulon Peninsula, Banten, Indonesia in five locations and three types of aquatic habitats i.e., natural ponds, streams in forests, and rivers. The transect line method is used to collect adult dragonflies. Thirty-one species of dragonflies belong to two anisopteran families and six zygopteran families were reported in this study. Twenty-two species were as new records based on previous publications. There were different species compositions in all habitat types (R = 1, p = 0.0016, one-way ANOSIM with Bray-Curtis similarity index). Natural ponds have the highest anisopteran species richness compared to other habitats. Eight species Anisoptera only were found in natural ponds. Meanwhile, zygopteran species prefer in flowing water habitat.
The effect of different concentrations of ammonium sulfate and pH extraction on the production of phycocyanin from Galdieria sp
Authors: Rahman D Y , Syafindra A M , Rosananda N , Sasongko A , Susilaningsih D 
Published: March 2020

Abstract
The alternative blue pigment of phycocyanin sources other than Spirulina is Galdieria from Cyanidiales order. Galdieria sp. is unicellular red microalgae which naturally found in volcanic area with a high temperature and low pH (0.5–3). In this study, Galdieria sp. 009 has grown autotrophically in Allen medium and on different concentrations of ammonium sulfate. The growth and in vivo phycocyanin content were quantified in the cultures. Phycocyanin was extracted using a physical method with different extraction pH (3, 5, and 7). Highest specific phycocyanin content up to 100 mg g−1 was observed in cell grown on Allen medium with two times ammonium sulfate concentration and extracted with pH 7. The different concentrations of ammonium sulfate used in the Allen medium impacted the yield of phycocyanin. Although extraction at pH 7 caused high phycocyanin content, the phycocyanin tends to have high thermostability (>60°C) and purity index at pH 5. The phycocyanin extracted from Galdieria sp.009 may potentially be an alternative blue food colorant beside Spirulina, as it has higher thermostability that Spirulina phycocyanin (>47°C).
Initial study of polymer-based nanoparticles effect on carotenogenesis of Haematococcus lacustris
Authors: Widyaningrum D , Ohama T 
Published: March 2020

Abstract
The carotenogenesis in Haematococcus lacustris, accompanying by encystment starting from green motile cells to aplanospores, is suggested mediating by reactive oxygen species (ROS). Our previous study showed that isobutyl-cyanoacrylate nanoparticles (IBCA- NPs) induced the cell mortality, preceding by intracellular ROS accumulation, in most of Volvocales species but not H. lacustris. This study aimed to investigate the effect of IBCA- NPs exposure to H. lacustris on carotenogenesis. The cells were cultured on C medium then exposed to 100 mg · L−1 -1 of 180 nm of IBCA-NPs for the 14 days. The accumulation of astaxanthin was started after four days of IBCA-NPs exposure. The ratio of intermediate cells reached to 46% after 14 days of IBCA-NPs exposure, while the ratio of intermediate cells of untreated culture was only 11%. The results suggested that the addition of nanoparticles could enhance the production of astaxanthin. Further studies are required to investigate the correlation between nanoparticle exposure and ROS generation that leads to the biosynthesis of astaxanthin. The condition of cell growth and the addition of nanoparticles shall be optimized to increase the production of ketocarotenoid and to minimalize the negative effect of longer exposure time of IBCA-NP that could lead the cell mortality.
Identification of anticancer compounds in leaves extracts of agarwood (Aquilaria malaccensis (Lamk.))
Authors: Millaty I N K , Wijayanti N , Hidayati L , Nuringtyas T R 
Published: March 2020

Abstract
Cancer is still become the main causes of death in the world. The leaves of Aquilaria malaccensis have an antioxidant and cytotoxic activity against several cancer cell lines. It can be developed as an alternative medicine. However, comprehensive information about the database of metabolites in A. malaccensis leaves is not yet available. This study aimed to screen the metabolites of chloroform and ethanol extracts of A. malaccensis, which has been reported in the database as an anticancer. Determination of metabolites contained in both chloroform and ethanol extracts were conducted using Gas Chromatography–Mass Spectrometry (GC–MS). GC–MS analysis detected nine metabolites in the chloroform extract while twenty one metabolites in the ethanol extract. The most common compounds observed was fatty acids and terpenoids. Among those detected, metabolites that have potential as anticancer in the chloroform extracts were 9–Hexadecanoic acid and Tetracosanoic acid. While in the ethanol extracts include 2,6–Octadien–1–ol, 3,7–dimethyl; 3,6–Octadecadiynoic acid, 3–Octadecyne, Lauric acid, Myristic acid, Nonadecanoic acid, Oleic Acid, Phytol, Loliolide dan Squalene. Further analysis to confirm which compounds most responsible for the anticancer activity in the A. malaccensis extract is planned as the next steps of this study.
Antioxidant activity of soluble protein from natural fermented buffalo milk
Authors: Kusumaningtyas E , Utami A 
Published: March 2020

Abstract
Curd from buffalo milk, called dadih, is famous fermented milk from West Sumatra, Indonesia. The milk is fermented naturally by relying on microorganisms which exist nature. Dadih is commonly consumed as breakfast or eaten together with rice. This study aimed to determine the antioxidant activities of dadih from different regency in West Sumatra by using ABTS, DPPH and Fe reducing power methods. The soluble protein of dadih was separated from insoluble protein and fat by centrifugation at 11,000 x g. The supernatant was then assayed for protein content and antioxidant activities. The result showed that among dadih from Agam, Sijunjung and Solok regency, dadih from Agam regency showed lowest protein content but highest antioxidant activities in Fe reducing power with absorbance 1.28 and scavenging activity against radical ABTS (86.46%) or DPPH (82.24%). The result indicated that dadih, especially from Agam regency is very potent as an antioxidant.
Selection and characterization yeast potential from pineapple for bioethanol production using some sugar sources
Authors: Chasanah N , Meryandini A , Sunarti T C 
Published: March 2020

Abstract
Bioethanol can be produced through fermentation process of sugars based on carbohydrate biomass such as lignosellulosa by microorganism such as yeast. Yeast changes sugar as a carbon source to produce ethanol and CO2 in anaerobic condition. Pineapple as a fruit commodity that is widely cultivated in Indonesia contain high sugar and carbohydrates so it might contain yeast which has the potential in bioethanol production. This research aims to select and characterize potential yeast from pineapple for bioethanol production from some sugars. The method included fermentation test, ethanol production (measurement of reduction sugar, dry cell biomass, and ethanol contain), and molecular identification. Yeast utilize substrates as carbon source for producing cell or ethanol. Ethanol production influenced by substrates, microorganism, and total cell. The results showed that NHC3 used as selected isolate based on the degree of coloration in Oxidative Fermentative Media. The largest ethanol production was 10,63 g/L obtained from mannose where the highest value of substrate utilization efficiency was 95%. The value of yield product is 0,27 g bioethanol/g substrate and yield biomass is 0,14 g biomass/g substrate. BLAST result shows NHC3 had homology >99% with Saccharomycetales and Kodamaea ohmeri.
Selection of potential Indonesian plant species for antioxidant
Authors: Kartini K , Setiawan F , Sukweenadhi J , Yunita O , Avanti C 
Published: March 2020

Abstract
Diabetic foot ulcer (DFU) is commonly complication of diabetes mellitus. Antimicrobial and antioxidant herbs can be used as complementary therapy in DFU and these properties will be strengthened by silver nanoparticles (AgNPs). This study was performed for screening of Indonesian herbs promising as antioxidant which can be subsequently involved in the green synthesis of AgNPs. Antioxidant of eight herbs extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl method. Their total group of compounds were quantified as well. Phyllanthus niruri and Orthosiphon stamineus exhibited the strongest antioxidant power with IC50 of 102 and 133 μg/ml, followed by Curcuma domestica, Stelechocarpus burahol, and Curcuma xanthorriza with the IC50 of 363, 481, and 540 μg/ml, respectively. Sonchus arvensis, Apium graveolens, Centella asiatica did not have antioxidants activity. Total phenolics from the highest is: O. stamineus > C. domestica > C. xanthorrizha > P. niruri > S. burahol > S. arvensis > A. graveolens > C. asiatica, whereas total flavonoids order is O. stamineus > S. burahol > P. niruri > A. graveolens > C. asiatica > S. arvensis > C. xanthorrizha > C. domestica. We concluded that O. stamineus was the most suitable herb to be used as a bioreductor in the green synthesis of AgNPs.
Isolation and characterization of indole-3-acetic acid producing bacteria from red onion rhizosphere
Authors: Zahroya I U , Mubarik N R , Tjahjoleksono A 
Published: March 2020

Abstract
There are many bacteria classified as growth enhancer in plants which known as Plant Growth Promoting Rhizobacteria (PGPR). PGPR can directly induce plant growth by producing of phytohormone Indole-3-Acetic Acid (IAA). The objective of this study was to obtain bacterial isolates of IAA producing rhizobacteria from red onion rhizosphere and to characterize the potential isolates. The methods used in the study were a sampling of soil, isolation of IAA producing rhizobacteria, measurement of IAA by using colorimetric assay, morphological identification of bacterial isolates, measurement of bacterial growth and IAA production, and test of hypersensitivity on tobacco leaves. There were fourteen IAA producing bacterial from red onion rhizosphere. The isolates could produce IAA by colorimetric assay detection. Three isolates produced IAA was higher than other isolates i.e. BIT 2,4 (61.72 ppm), BIS 3,4 (60.92 ppm) and BIT 2,1 (49.3 ppm). Meanwhile, isolate BIT 4,1 produced the lowest IAA as much as 3 ppm. Four isolates were gram-positive bacteria. Isolates BIS 3,4 and BIT 2,1 produced exogenous (extracellular) IAA on stationary phase. Two isolates potential (BIS 3,4 and BIT 2,1) did not cause necrotic symptoms or negative results for the hypersensitivity test on tobacco leaves.
Bioprospecting of fruit extract and endophytic bacteria isolated from dewandaru (Eugenia uniflora L.) as antibacterial against colorectal bacteria
Authors: Indrawati I , Safitri F A L , Rossiana N 
Published: March 2020

Abstract
Colorectal cancer (CRC) is the third most commonly diagnosed malignancy and the fourth leading cause of cancer-related deaths in the world. The gut bacteria are an important player in the development of colorectal cancer. Dewandaru (Eugenia uniflora L.) have been used as a traditional medicine to treat various diseases. Many of antibacterial metabolites produced by the fruit and its endophytic bacteria. This study aimed to find Antibacterial activity of ethanolic extract of dewandaru fruit using Kirby - Bauer disk diffusion method and to determine Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) using Dilution Tube Method (DTM). Endophytic bacteria were isolated from dewandaru fruit by using sterilization treatment followed by serial dilution agar plate and streak technique. All the isolates and ethanolic extract with different concentration were evaluated for the antimicrobial activity against colorectal bacteria (Streptococcus bovis, Enterococcus faecalis, Escherichia coli and Salmonella enterica). The results showed resistant– strong inhibitory zone category of fruit extract and isolated endophytic bacteria. The isolates were identified as Bacillus cereus, Bacillus amyloliquefaciens, and Bacillus sp.
Screening and characterization of molecular markers for sugarcane stem borer (Saccharum officinarum L.) resistance genes
Authors: Aristya G R , Larasati B , Prabowo H , Husna N , Kasiamdari R S 
Published: March 2020

Abstract
Sugarcane (Saccharum officinarum L.) constitutes a main crop for production of sugar that serves as the main calorie source for public consumption. It is a particular challenge for the Government and related parties to ensure its availability. A decline in sugarcane production on agricultural land occurs due to disturbances by plant pests, especially stem borers. Stem borers can reduce sugar production by 52 to 73%. The purposes of the present study were to screen and detect genes resistant to stem borer attacks in 22 sugarcane cultivars and to characterize SacBBI4 as among the resistance gene markers in sugarcane. The present study produced 3 primer designs. The primer P-SacBBI4-A successfully amplified SacBBI4 gene at 729 bp, the primer P-SacBBI4-B at 624 bp, while the primer P-SacBBI4-C successfully amplified the target gene at 671 bp and same value were also obtained when analyzing the sequences on the Genstudio. As shown by the phylogenetic tree, two large clusters were formed. The first cluster was the WIP1 coding cluster, while the second one was the BBI coding cluster. The phylogenetic tree and genetic distance showed that the primer P-SacBBI4-C was the primer design closest to the target gene SacBBI4 on the NCBI data.
Introgression of bacterial blight resistance gene Xa7 into popular Indonesian rice varieties through backcross and molecular breeding
Authors: Hairmansis A , Warsono  , Supartopo  , Yullianida  , Nasution A , Utami D W , Suwarno  
Published: March 2020

Abstract
Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of major diseases in rice production. The BB resistance gene Xa7 is one of effective genes against Xoo strains in Indonesia. This study aimed to introgress BB resistance gene Xa7 into submergence tolerant rice variety Inpara 5 (IR64 Sub1) and brown plant hopper (BPH) resistant rice variety Inpari 13. Stable BB resistant rice lines have been successfully developed after three backcrossing and four generations of fixation (BC3F4). Screening for BB resistance were conducted in generative stage using Xoo strains IV and VIII. The reaction pattern of the backcross lines against these two Xoo strains were similar to the BB resistant parent Conde. The introgression of the Xa7 gene in Inpara 5 and Inpari 13 genome have been confirmed by using SSR markers RM20589 and RM20590; and Xa7 gene specific marker Xa7-LD34. Agronomic characters and grain quality properties of the backcross lines were comparable to the recurrent parents. Other desirable traits from recurrent parents including submergence tolerance and BPH resistance retained in the selected backcross lines. The BB resistance rice lines with popular rice varieties genetic background developed through this study have potential to be released for farmers adoption.
Influence of phosphorus and organic fertilizers on the growth and yield of Indonesian new superior variety of soybean in dry climate rice fields
Authors: Herawati N , Hidayah B N , Ghulammahdi M 
Published: March 2020

Abstract
The response of soybean crop to organic fertilizers has not been fully understood in Indonesia. This research aims to investigate the response of new superior variety of Anjasmoro soybean to phosphorus and organic fertilizers in the dry climate ricefields. The experiment was conducted in Sesela Village, West Nusa Tenggara Province, Indonesia from August - November 2015 using a randomized 2-factor design. The first factor was phosphorus fertilizer with 4 levels: 0 kg/ha, 36 kg/ha, 72 kg/ha and 108 kg/ha. The second factor was organic fertilizer with 4 levels of fertilizer dosage: 0 ton/ha, 2.5 tons/ha, 5 tons/ha and 7.5 tons/ha. Parameter observed were plant height, number of leaves, number of nodules, number of productive branches, number of pods, number of empty pods, dry weight, weight of 100 seeds, and production. The results showed that the optimum dose of organic fertilizer was 7.5 tons/ha with the highest number of pods of 100. This was confirmed by the regression value (R2) of 0.97 which indicates that organic fertilizer affects the increase in the number of pods in a quadratic. Phosphorus fertilizer has an effect on the number of empty pods, number of productive pods, number of pods, productivity and production of soybean.