bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term impacts of mid-Holocene drier climatic conditions on Bolivian tropical dry forests
Authors: Plumpton Heather J. , Mayle Francis E. , Whitney Bronwen S. 
Published: October 2019

AbstractThe Bolivian Chiquitano dry forest is the largest block of intact seasonally dry tropical forest in South America and is a priority ecoregion for conservation due to its high threat status. However, the long-term impacts of drier climatic conditions on tropical dry forests are not well understood, despite climate models predicting increased droughts over Bolivia in the coming century. In this paper, we assess the impacts of drier climatic conditions during the mid-Holocene on the Bolivian Chiquitano tropical dry forest using fossilised pollen, phytoliths, macro-charcoal, and geochemical proxies from a sediment core from a large lake (Laguna Mandioré) on the Bolivia–Brazil border. Our results show that drier climatic conditions during the mid-Holocene caused a local-scale, ecotonal expansion of upland savannah at the expense of dry forest. Interaction between drier climatic conditions and fire regime likely exerted a stronger control over the position of the dry forest–savannah ecotone than edaphic factors. However, the majority of the dry forest within the lake catchment maintained a closed canopy throughout the drier conditions of the mid-Holocene, despite floristic turnover towards more drought-tolerant taxa. These findings imply overall resilience of the Chiquitano dry forest biome to future drought, albeit with floristic changes and upland savannah encroachment at ecotones.
Late Miocene changes in C3, C4 and aquatic plant vegetation in the Indus River basin: evidence from leaf wax δ13C from Indus Fan sediments
Authors: Suzuki Kenta , Yamamoto Masanobu , Seki Osamu 
Published: October 2019

AbstractVegetation changes in the Indus River basin within the past 10.8 million years were investigated based on the analysis of n-fatty acids and their carbon isotopes in sediments from IODP Site U1457 in the Laxmi Basin of the Arabian Sea. The δ13C of long-chain n-C32 fatty acid shifted from −34 to −22 ‰ from 10 to 6.3 Ma, while the δ13C of mid-chain n-C24 fatty acid was nearly constant at around −23 to −22 ‰ over the same period. This large difference in the δ13C values suggests that the mid-chain fatty acids reflect the contribution of aquatic vascular C3 plants. Before 6.3 Ma, the average chain length of n-fatty acids and the δ13C values of long-chain fatty acids were negatively correlated, suggesting that the δ13C values reflected the relative abundance of terrestrial C3 versus aquatic C3 plants in the Indus River basin and western India. After 5.8 Ma, the average chain length was variable, but the δ13C values remained nearly the same, suggesting that the δ13C values reflected heavier δ13C values of both aquatic C3 and C4 plants. A three-end-member model calculation suggests that terrestrial C3 plants were replaced by C4 plants in the Indus River basin and western India from 9.7 or 8.2 to 6.3 Ma. Aridification in those areas during the late Miocene period may have driven the replacement of terrestrial C3 plants by C4 plants. An episodic increase in the abundance of terrestrial plants around 8 Ma is attributed to elevated precipitation by regionally enhanced moisture transport.
Social inclusion of people with dementia – an integrative review of theoretical frameworks, methods and findings in empirical studies
Authors: Pinkert Christiane , Köhler Kerstin , von Kutzleben Milena , Hochgräber Iris , Cavazzini Christoph , Völz Silke , Palm Rebecca , Holle Bernhard 
Published: October 2019

AbstractThe social inclusion of people with dementia (PwD) is recognised as a global goal of legislation, societal initiatives and service provision. Ensuring the social inclusion of PwD in these areas implies that its dimensions and domains are clear and unambiguous. However, the concept of social inclusion as it is currently used by researchers and practitioners is often vague or acts as a container concept for a variety of different approaches. This paper reports on an integrative review that analysed qualitative and quantitative studies on social inclusion and exclusion of PwD. It focused not only on the empirical results of the included studies but also on the theoretical embedding and methodological approaches to the concept of social inclusion and exclusion. We find that empirical studies on the social inclusion of PwD are scarce and largely characterised by a lack of or inconsistent conceptualisation. Against this background, the operationalisation of the concept and the assessment of the individual aspects of social inclusion with standardised instruments seem to be premature. Substantial theoretical and methodological work is needed to guide research on the social inclusion of PwD. The empirical results show that relationships with other people and being integrated into social networks are essential aspects of social inclusion. Likewise, the strategies and attitudes of caring persons can help to create or reinforce exclusion.
What contributes to successful whole-class Ensemble Tuition?
Authors: Hallam Susan 
Published: October 2019

AbstractIn 2001, the government in England pledged in the white paper Schools Achieving Success that all primary school pupils who wanted to should have the opportunity to learn to play a musical instrument. The research reported here aimed to establish what contributed to the success of the implementation of this policy. The findings showed that success depended on all school staff being committed to the programme; children having experience of high-quality provision; opportunities to participate in performance; and accessible progression routes.
A Simple and Rapid Staining Technique for Sex Determination of Trichinella Larvae Parasites by Confocal Laser Scanning Microscopy
Authors: Gavarane Inese , Kirilova Elena , Rubeniņa Ilze , Mežaraupe Ligita , Osipovs Sergejs , Deksne Gunita , Pučkins Aleksandrs , Kokina Inese , Bulanovs Andrejs , Kirjušina Muza 
Published: October 2019

AbstractThe roundworms of Trichinella genus are worldwide distributed and their prevalence in nature is high. Trichinella genus parasites are the causative agents of foodborne zoonosis trichinellosis. The main prevention and control of the infection are meat inspection by the magnetic stirrer method for the detection of Trichinella larvae in muscle samples. The treatment can be effective if the parasite is discovered early in the intestinal phase. Once the Trichinella larva has reached the muscle tissue, the parasite remains therein and there is no treatment for this life cycle stage. The Trichinella species is dioecious with separate male and female individuals. The developed staining technique that uses confocal laser scanning microscopy (CLSM) displays sufficient results for Trichinella larvae examination and this protocol is applicable to study the internal and external structures and for the sex determination of T. britovi and T. spiralis larvae samples. In the present study, a luminescent derivative was synthesized and used for staining of T. spiralis and T. britovi larvae samples for the examination by CLSM. Various fixatives, such as AFA, 70% ethanol, and Bouin's and Carnoy's solutions were tested for sample preparation. The synthesized luminescent compound demonstrates best visualization results for samples fixed in Bouin's fixative.
In VivoMultivesicular Body and Exosome Secretion in the Intestinal Epithelial Cells of Turtles During Hibernation
Authors: Vistro Waseem Ali , Huang Yufei , Bai Xuebing , Yang Ping , Haseeb Abdul , Chen Hong , Liu Yifei , Yue Zhang , Tarique Imran , Chen Qiusheng 
Published: October 2019

AbstractThe present study was designed to investigate thein vivobiological processes of multivesicular bodies (MVBs) and exosomes in mitochondria-rich cells (MRCs), goblet cells (GCs), and absorptive cells (ACs) in turtle intestines during hibernation. The exosome markers, cluster of differentiation 63 (CD63) and tumor susceptibility gene 101 (TSG101), were positively expressed in intestinal villi during turtle hibernation. The distribution and formation processes of MVBs and exosomes in turtle MRCs, GCs, and ACs were further confirmed by transmission electron microscopy. During hibernation, abundantly secreted early endosomes (ees) were localized in the luminal and basal cytoplasm of the MRCs and ACs, and late endosomes (les) were dispersed with the supranuclear parts of the MRCs and ACs. Many “heterogeneous” MVBs were identified throughout the cytoplasm of the MRCs and ACs. Interestingly, the ees, les, and MVBs were detected in the cytoplasm of the GCs during hibernation; however, they were absent during nonhibernation. Furthermore, the exocytosis pathways of exosomes and autophagic vacuoles were observed in the MRCs, GCs, and ACs during hibernation. In addition, the number of different MVBs with intraluminal vesicles (ILVs) and heterogeneous endosome–MVB–exosome complexes was significantly increased in the MRCs, GCs, and ACs during hibernation. All these findings indicate that intestinal epithelial cells potentially perform a role in the secretion of MVBs and exosomes, which are essential for mucosal immunity, during hibernation.
Spatial Accessibility and Social Inclusion: The Impact of Portugal's Last Health Reform
Authors: Lopes H. S. , Ribeiro V. , Remoaldo P. C. 
Published: October 2019

AbstractHealth policies seek to promote access to health care and should provide appropriate geographical accessibility to each demographical functional group. The dispersal demand of health‐care services and the provision for such services at fixed locations contribute to the growth of inequality in their access. Therefore, the optimal distribution of health facilities over the space/area can lead to accessibility improvements and to the mitigation of the social exclusion of the groups considered most vulnerable. Requiring for such, the use of planning practices joined with accessibility measures. However, the capacities of Geographic Information Systems in determining and evaluating spatial accessibility in health system planning have not yet been fully exploited. This paper focuses on health‐care services planning based on accessibility measures grounded on the network analysis. The case study hinges on mainland Portugal. Different scenarios were developed to measure and compare impact on the population's accessibility. It distinguishes itself from other studies of accessibility measures by integrating network data in a spatial accessibility measure: the enhanced two‐step floating catchment area. The convenient location for health‐care facilities can increase the accessibility standards of the population and consequently reduce the economic and social costs incurred. Recently, the Portuguese government implemented a reform that aimed to improve, namely, the access and equity in meeting with the most urgent patients. It envisaged, in terms of equity, the allocation of 89 emergency network points that ensured more than 90% of the population be within 30 min from any one point in the network. Consequently, several emergency services were closed, namely, in rural areas. This reform highlighted the need to improve the quality of the emergency care, accessibility to each care facility, and equity in their access. Hence, accessibility measures become an efficient decision‐making tool, despite its absence in effective practice planning. According to an application of this type of measure, it was possible to verify which levels of accessibility were decreased, including the most disadvantaged people, with a larger time of dislocation of 12 min between 2001 and 2011.
Maximum Sprite Streamer Luminosity Near the Stratopause
Authors: Füllekrug Martin , Nnadih Stanislaus , Soula Serge , Mlynarczyk Janusz , Stock Micheal , Lapierre Jeff , Kosch Michael 
Published: October 2019

AbstractSprites are composed of numerous streamers which exhibit transient luminosities in the upper middle atmosphere above thunderclouds after initiation by an intense positive lightning discharge, often followed by lightning continuing current. Here we report the discovery of a sprite which exhibits its main luminosity near the stratopause. This novel phenomenon is attributed to a sudden surge of intracloud lightning leader activity, based on a rigorous analysis of our observed electromagnetic waveforms. Each lightning leader discharge causes an additional electric field that generates a small amount of electromagnetic energy near the stratopause and thereby contributes to the overall sprite luminosity morphology. The observation of sprite streamers near the stratopause is important because it is relevant for the ongoing assessment of the lightning impact on N2 and CO2 with emissions from the near to far infrared part of the spectrum.
Provenance of Block Fields Along Lunar Wrinkle Ridges
Authors: French Renee A. , Watters Thomas R. , Robinson Mark S. 
Published: October 2019

AbstractBlock fields and associated relatively high reflectance material along wrinkle ridge summits are revealed in meter‐scale Lunar Reconnaissance Orbiter Camera images. Wrinkle ridges with block fields in Mare Australe, Crisium, Fecunditatis, Frigoris, Nubium, the northern half of Oceanus Procellarum, Serenitatis, and Tranquillitatis are evenly distributed, and block density generally increases with wrinkle ridge slope. The median cross‐sectional area (proxy for diameter) of the 1,368 blocks measured in this study is ~7 m2, and 75% of these blocks are ≤~14 m2. We propose that the largest variation in cross‐sectional area of the blocks is related to substrate physical properties of the mare basalt and not a function of the slope on which they occur, suggesting that physical properties rather than slope are a stronger control on block size. The maximum length of blocks may constrain the minimum basalt flow thickness or joint width; our block measurements suggest basalt flows ~2–14 m thick, agreeing with previous estimates. The data suggest that blocks originate from mare basalt layers that buckle and break as a result of movement along ridge‐forming thrust faults. High reflectance material associated with wrinkle ridge blocks likely represents freshly exposed rock and soil. Meter‐scale blocks may erode relatively quickly due to collisional disruption, indicating recent downslope movement of regolith exposing preexisting blocks or blocks formed and exposed by recent activity on ridge‐forming faults.
Further evidence of involvement of TMEM132E in autosomal recessive nonsyndromic hearing impairment
Authors: Liaqat Khurram , Hussain Shabir , Bilal Muhammad , Nasir Abdul , Acharya Anushree , Ali Raja Hussain , Nawaz Shoaib , Umair Muhammad , Schrauwen Isabelle , Ahmad Wasim , Leal Suzanne M. 
Published: October 2019

AbstractAutosomal-recessive (AR) nonsyndromic hearing impairment (NSHI) displays a high degree of genetic heterogeneity with >100 genes identified. Recently, TMEM132E, which is highly expressed in inner hair cells, was suggested as a novel ARNSHI gene for DFNB99. A missense variant c.1259G>A: p.(Arg420Gln) in TMEM132E was identified that segregated with ARNSHI in a single Chinese family with two affected members. In the present study, a family of Pakistani origin with prelingual profound sensorineural hearing impairment displaying AR mode of inheritance was investigated via exome and Sanger sequencing. Compound heterozygous variants c.382G>T: p.(Ala128Ser) and c.2204C>T: p.(Pro735Leu) in TMEM132E were observed in affected but not in unaffected family members. TMEM132E variants identified in this and the previously reported ARNSHI family are located in the extracellular domain. In conclusion, we present a second ARNSHI family with TMEM132E variants which strengthens the evidence of the involvement of this gene in the etiology of ARNSHI.
No more helper adenovirus: production of gutless adenovirus (GLAd) free of adenovirus and replication-competent adenovirus (RCA) contaminants
Authors: Lee Dongwoo , Liu Jida , Junn Hyun Jung , Lee Eun-Joo , Jeong Kyu-Shik , Seol Dai-Wu 
Published: October 2019

AbstractGene therapy is emerging as an effective treatment option for various inherited genetic diseases. Gutless adenovirus (GLAd), also known as helper-dependent adenovirus (HDAd), has many notable characteristics as a gene delivery vector for this particular type of gene therapy, including broad tropism, high infectivity, a large transgene cargo capacity, and an absence of integration into the host genome. Additionally, GLAd ensures long-term transgene expression in host organisms owing to its minimal immunogenicity, since it was constructed following the deletion of all the genes from an adenovirus. However, the clinical use of GLAd for the treatment of inherited genetic diseases has been hampered by unavoidable contamination of the highly immunogenic adenovirus used as a helper for GLAd production. Here, we report the production of GLAd in the absence of a helper adenovirus, which was achieved with a helper plasmid instead. Utilizing this helper plasmid, we successfully produced large quantities of recombinant GLAd. Importantly, our helper plasmid-based system exclusively produced recombinant GLAd with no generation of helper plasmid-originating adenovirus and replication-competent adenovirus (RCA). The recombinant GLAd that was produced efficiently delivered transgenes regardless of their size and exhibited therapeutic potential for Huntington’s disease (HD) and Duchenne muscular dystrophy (DMD). Our data indicate that our helper plasmid-based GLAd production system could become a new platform for GLAd-based gene therapy.
PR55α regulatory subunit of PP2A inhibits the MOB1/LATS cascade and activates YAP in pancreatic cancer cells
Authors: Hein Ashley L. , Brandquist Nichole D. , Ouellette Caroline Y. , Seshacharyulu Parthasarathy , Enke Charles A. , Ouellette Michel M. , Batra Surinder K. , Yan Ying 
Published: October 2019

AbstractPP2A holoenzyme complexes are responsible for the majority of Ser/Thr phosphatase activities in human cells. Each PP2A consists of a catalytic subunit (C), a scaffold subunit (A), and a regulatory subunit (B). While the A and C subunits each exists only in two highly conserved isoforms, a large number of B subunits share no homology, which determines PP2A substrate specificity and cellular localization. It is anticipated that different PP2A holoenzymes play distinct roles in cellular signaling networks, whereas PP2A has only generally been defined as a putative tumor suppressor, which is mostly based on the loss-of-function studies using pharmacological or biological inhibitors for the highly conserved A or C subunit of PP2A. Recent studies of specific pathways indicate that some PP2A complexes also possess tumor-promoting functions. We have previously reported an essential role of PR55α, a PP2A regulatory subunit, in the support of oncogenic phenotypes, including in vivo tumorigenicity/metastasis of pancreatic cancer cells. In this report, we have elucidated a novel role of PR55α-regulated PP2A in the activation of YAP oncoprotein, whose function is required for anchorage-independent growth during oncogenesis of solid tumors. Our data show two lines of YAP regulation by PR55α: (1) PR55α inhibits the MOB1-triggered autoactivation of LATS1/2 kinases, the core member of the Hippo pathway that inhibits YAP by inducing its proteasomal degradation and cytoplasmic retention and (2) PR55α directly interacts with and regulates YAP itself. Accordingly, PR55α is essential for YAP-promoted gene transcriptions, as well as for anchorage-independent growth, in which YAP plays a key role. In summary, current findings demonstrate a novel YAP activation mechanism based on the PR55α-regulated PP2A phosphatase.
Drivers of the composition of active rhizosphere bacterial communities in temperate grasslands
Authors: Vieira Selma , Sikorski Johannes , Dietz Sophie , Herz Katharina , Schrumpf Marion , Bruelheide Helge , Scheel Dierk , Friedrich Michael W , Overmann Jörg 
Published: October 2019

Abstract
The active bacterial rhizobiomes and root exudate profiles of phytometers of six plant species growing in central European temperate grassland communities were investigated in three regions located up to 700 km apart, across diverse edaphic conditions and along a strong land use gradient. The recruitment process from bulk soil communities was identified as the major direct driver of the composition of active rhizosphere bacterial communities. Unexpectedly, the effect of soil properties, particularly soil texture, water content, and soil type, strongly dominated over plant properties and the composition of polar root exudates of the primary metabolism. While plant species-specific selection of bacteria was minor, the RNA-based composition of active rhizosphere bacteria substantially differed between rhizosphere and bulk soil. Although other variables could additionally be responsible for the consistent enrichment of particular bacteria in the rhizosphere, distinct bacterial OTUs were linked to the presence of specific polar root exudates independent of individual plant species. Our study also identified numerous previously unknown taxa that are correlated with rhizosphere dynamics and hence represent suitable targets for future manipulations of the plant rhizobiome.
Caspase-8, receptor-interacting protein kinase 1 (RIPK1), and RIPK3 regulate retinoic acid-induced cell differentiation and necroptosis
Authors: Someda Masataka , Kuroki Shunsuke , Miyachi Hitoshi , Tachibana Makoto , Yonehara Shin 
Published: October 2019

AbstractAmong caspase family members, Caspase-8 is unique, with associated critical activities to induce and suppress death receptor-mediated apoptosis and necroptosis, respectively. Caspase-8 inhibits necroptosis by suppressing the function of receptor-interacting protein kinase 1 (RIPK1 or RIP1) and RIPK3 to activate mixed lineage kinase domain-like (MLKL). Disruption ofCaspase-8expression causes embryonic lethality in mice, which is rescued by depletion of eitherRipk3orMlkl, indicating that the embryonic lethality is caused by activation of necroptosis. Here, we show that knockdown ofCaspase-8expression in embryoid bodies derived from ES cells markedly enhances retinoic acid (RA)-induced cell differentiation and necroptosis, both of which are dependent onRipk1andRipk3; however, the enhancement of RA-induced cell differentiation is independent ofMlkland necrosome formation. RA treatment obviously enhanced the expression of RA-specific target genes having the retinoic acid response element (RARE) in their promoter regions to induce cell differentiation, and induced marked expression of RIPK1, RIPK3, and MLKL to stimulate necroptosis.Caspase-8knockdown induced RIPK1 and RIPK3 to translocate into the nucleus and to form a complex with RA receptor (RAR), and RAR interacting with RIPK1 and RIPK3 showed much stronger binding activity toRAREthan RAR without RIPK1 or RIPK3. InCaspase-8-deficient as well asCaspase-8- andMlkl-deficient mouse embryos, the expression of RA-specific target genes was obviously enhanced. Thus, Caspase-8, RIPK1, and RIPK3 regulate RA-induced cell differentiation and necroptosis both in vitro and in vivo.