bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barriers of Antenatal Care Service Utilization in Somali Regional State Using Social Ecological Framework, Eastern Ethiopia, Ethiopia: a Qualitative Study
Authors: Mohammed Abdurehman , Teklu Alula , Beyene Senait , Hashi Abdiwahab , Abebe Zerihun , Gezahegn Wondimagegn , Mulugeta Mengestie , Metaferia Gesit , Shekabdulahi Muktar , Tolera Moti , Gebru Teklemichael 
Published: January 2020

Abstract
Background: A good Antenatal Care during pregnancy is important to ensure the health of the mother and the healthy development of the fetus. In 2016, at Ethiopian Somali region (43.6 %) of pregnant mothers had an Antenatal Care (ANC) visit at least once during their last pregnancy and only (11.8%) had a history of four or more visits for Antenatal Care, which are the lowest rates among all regions in Ethiopia.Objective: The objective of the study was to explore the barriers and determine enabling factors affecting Antenatal Care service utilization in Somali Regional state.Methods: A qualitative exploration was conducted from February 18 to March 11, 2017. A pre-tested semi-structured interview guides, and facility abstractions with checklists were used to collect the data. A total of 31 individual interviews, 12 focus group discussions, and 21 facility abstractions were collected. The population of the region were stratified into three areas based on their settlement characteristics as agro-pastoralist, pastoralist, and urban. Two districts were selected from each category based on their performance. The data was entered, coded, categorized, and analyzed by utilizing Nvivo version 11software. A thematic analysis was conducted using themes that were developed based on the constructs of the socio-ecological model.Results: According to the study, economic constraints, place of residence, mothers’ being lack of awareness, preference of female health care provider, husband’s disapproval, lack of acceptance by the community, absence of full ANC services packages related with shortage of manpower, medical supply in majority of the health facilities and lack of perceived benefits from ANC service were the main barriers of ANC service utilization. Conclusion: Individual, interpersonal, and organizational level factors were the most dominant barriers for ANC service utilization. Therefore, FMOH, regional, zonal and district level health administrators and other stakeholders should design effective IEC, community mobilization and work on supply-related problems to .increase ANC utilization in the region.
Evolutionary history of two cryptic species of Northern African jerboas
Authors: Moutinho Ana Filipa , Serén Nina , Paupério Joana , Silva Teresa Luísa , Martínez-Freiría Fernando , Sotelo Graciela , Faria Rui , Mappes Tapio , Alves Paulo Célio , Brito José Carlos , Boratyński Zbyszek 
Published: January 2020

Abstract
Background: Climatic variation and geologic change both play significant roles in shaping species distributions, thus affecting their evolutionary history. In Sahara-Sahel, climatic oscillations shifted the desert extent during the Pliocene-Pleistocene interval, triggering the diversification of several species. Here, we investigated how these biogeographical and ecological events have shaped patterns of genetic diversity and divergence in African Jerboas, desert specialist rodents. We focused on two sister and cryptic species, Jaculus jaculus and J. hirtipes, where we (1) evaluated their genetic differentiation, (2) reconstructed their evolutionary and demographic history; (3) tested the level of gene flow between them, and (4) assessed their ecological niche divergence.Results: The analyses based on 231 individuals sampled throughout North Africa, 8 sequence fragments (one mitochondrial and seven single copy nuclear DNA, including two candidate genes for fur coloration: MC1R and Agouti), 6 microsatellite markers and ecological modelling revealed: (1) two distinct genetic lineages with overlapping distributions, in agreement with their classification as different species, J. jaculus and J. hirtipes, with (2) low levels of gene flow and strong species divergence, (3) high haplotypic diversity without evident geographic structure within species, and (4) a low level of large-scale ecological divergence between the two taxa, suggesting species micro-habitat specialization. Conclusions: Overall, our results suggest a speciation event that occurred during the Pliocene-Pleistocene transition. The contemporary distribution of genetic variation suggests ongoing population expansions. Despite the largely overlapping distributions at a macrogeographic scale, our genetic results suggest that the two species remain reproductively isolated, as only negligible levels of gene flow were observed. The overlapping ecological preferences at a macro-geographic scale and the ecological divergence at the micro-habitat scale suggest that local adaptation may have played a crucial role in the speciation process of these species.
A Probabilistic Approach for Economic Evaluation of Occupational Health and Safety Interventions: A Case Study of Silica Exposure Reduction Interventions in the Construction Sector
Authors: Mofidi Amirabbas , Tompa Emile , Mortazavi SeyedBagher , Esfahanipour Akbar , Demers Paul A. 
Published: January 2020

Abstract

Background:Construction workers are at a high risk of exposure to various types of hazardous substances such as crystalline silica. Though multiple studies indicate the evidence regarding the effectiveness of different silica exposure reduction interventions in the construction sector, the decisions for selecting a specific silica exposure reduction intervention are best informed by an economic evaluation. Economic evaluation of interventions is subjected to uncertainties in practice, mostly due to the lack of precise data on important variables. In this study, we aim to identify the most cost-beneficial silica exposure reduction intervention for the construction sector under uncertain situation.Methods:We apply a probabilistic modeling approach that covers a large number of variables relevant to the cost of lung cancer, as well as the costs of silica exposure reduction interventions. To estimate the societal lifetime cost of lung cancer, we use an incidence cost approach. To estimate the net benefit of each intervention, we compare the expected cost of lung cancer cases averted, with expected cost of implementation of the intervention in one calendar year. Sensitivity analysis is used to quantify how different variables effects interventions net benefit.Results:A positive net benefit is expected for all considered interventions. The highest number of lung cancer cases are averted by combined use of wet method, local exhaust ventilation and personal protective equipment, about 107 cases, with expected net benefit of $45.9 million. Results also suggest that the level of exposure is an important determinant for the selection of the most cost-beneficial intervention.Conclusions:This study provides important insights for decision makers about silica exposure reduction interventions in the construction sector. It also provides an overview of the potential advantages of using probabilistic modeling approach to undertake economic evaluations, particularly when researchers are confronted with a large number of uncertain variables.
Prevalence of postpartum depression and associated factors among postnatal care attendees in Debre Berhan, Ethiopia, 2018.
Authors: dargie abate , Engidaw Nigus Alemnew , Gizachew Kefyalew Dagne 
Published: January 2020

Abstract
Background: Postpartum depression explains various groups of depressive symptoms and syndromes that can take place during the first 6 weeks following birth. The postpartum period is a critical time where both mild and severe mood disorders can occur. The familiar forms are baby blues and postpartum depression. Understanding the prevalence and associated factors of postpartum depression is mandatory for early detection and treatment.
Methods: Institution based cross-sectional study was conducted from 1st May to June 30, 2018. The study participants were eligible women who came to Debre Berhan referral hospital and health centers for postnatal care and vaccination service. The Edinburgh postnatal depression scale was used to assess postpartum depression. A systematic random sampling technique was used to collect the data after determining the skip fraction (k=2). The collected data were coded and entered into Epi-info version 7 and transported to SPSS version 20 for analysis. Both bivariate and multivariate binary logistic regression were done to identify associated factors. During bivariate analysis, variables with p-value < 0.05 were included in multivariate analysis. Odds ratios and their 95% confidence intervals were computed and variables with p-value less than 0.05 were considered to declare significantly associated factors (multivariate analysis).
Results: A total of 308 mothers who attended postpartum care included, which was a 100% response rate. The prevalence of postpartum depression was found to be 15.6% ( 95%CI=11.7, 19.8). Being widowed/widower, have poor social support, have currently hospitalized child, and had experienced a death of family member or close relative were significantly associated with postpartum depression.
Conclusions: the prevalence of postpartum depression was lower than most studies done in different areas. Major life events and traumas are associated with an increased risk of postpartum depression. Health professionals should be aware of the mother’s circumstances during the puerperium, they should initiate support to reduce the risk of depression in the postpartum period. Health care professionals working postpartum care clinics should give special attention to mothers who were widowed/widower, have poor social support, have currently hospitalized children, and had experienced a death of a family member or close relative.
Occurrence, distribution and virulence factors of clinically important Acinetobacter species recovered from selected freshwater resources in the Eastern Cape Province, South Africa
Authors: Adewoyin Mary Ayobami , Okoh Anthony Ifeanyi 
Published: January 2020

Abstract
Background : Several Acinetobacter species live in different ecosystems such as soil, freshwater, wastewater, and solid wastes. In this study, we assessed the occurrence of A. baumannii and A. nosocomialis , which are the major two clinically important species of the genus Acinetobacter , in three freshwater resources (Great Fish, Keiskemma, and Tyhume rivers) in the Eastern Cape Province, South Africa over a one year sampling regime (April 2017 - March 2018). Presumptive Acinetobacter species were subjected to molecular identification by using Acinetobacter genus-specific primers targeting the recA gene. The confirmed Acinetobacter species were further delineated into A. baumannii and A. nosocomialis using species-specific primer sets. Similarly, virulence genes, namely; afa/draBC, epsA, fimH, OmpA, PAI, sfa/focDE , and traT in the two Acinetobacter species were also determined using molecular techniques. Result : A total of 1107 presumptive Acinetobacter isolates were recovered from the freshwater resources of which 844 was confirmed positive for the Acinetobacter genus. Of the 844 Acinetobacter isolates, 285 (77%), 219 (70.9%) and 340 (79%) were recovered from Great Fish, Keiskemma and Tyhume rivers respectively. Our finding revealed that 410 (48.58%) and 23 (2.7%) of the isolates were confirmed to be A. baumannii and A. nosocomalis , respectively. The presence of these clinically-important Acinetobacter species in the freshwater studied suggests possible contamination of the selected rivers and also that A. baumannii and A. nosocomialis can thrive in aquatic environments. Besides, 308 (75.12%) A. baumannii and 3 (13.04%) A. nosocomialis isolates exhibited one or more virulence genes out of the seven tested, whereas 102 (24.88%) and 20 (86.95%) of the A. baumannii and A. nosocomialis isolates did not harbour any virulence gene. Additionally, OmpA was the most prevalent (p<0.05) virulence gene in A. baumannii with 69 (45.10%), 52 (50.98%) and 77 (49.68%) isolates in Great Fish, Keiskamma and Tyhume rivers respectively. Conclusion : The occurrence of these pathogens in rivers which are consumed by humans and livestock, as well as being used for irrigation system constitutes a risk to public health. Keywords: Freshwater resources, Molecular characterisation, Acinetobacter species, virulence gene s.
Impact of a Postcrash First Aid Educational Program on Knowledge, Perceived Skills Confidence, and Skills Utilization Among Traffic Police Officers: A Single-Arm Before-After Intervention Study
Authors: Ndile Menti Lastone , Lukumay Gift G. , Bolenius Karin , Outwater Anne H. , Saveman Britt-Inger , Backteman-Erlanson Susann 
Published: January 2020

Abstract

Background An overwhelming proportion of road traffic deaths and injuries in low- and middle-income countries (LMICs) occur in prehospital environments. Lay first responders such as police officers play an important role in providing initial assistance to victims of road traffic injuries either alone or in collaboration with others. The present study evaluated a postcrash first aid (PFA) educational program developed for police officers in Tanzania. Method A 16-hour PFA educational program was conducted in Dar es Salaam, Tanzania, for 135 police officers. Participants completed training surveys before, immediately and six months after the training (before, N = 135; immediately after, N = 135; after six months, N = 102). The primary outcome measures were PFA knowledge, perceived skills confidence, and skills utilization. Parametric and nonparametric tests were used to analyse changes in outcome. Results The mean PFA knowledge score increased from 44.73% before training ( SD = 20.70) to 72.92% six months after training ( SD = 18.12), p ˂ .001, N = 102. The mean PFA perceived skills confidence score (measured on a 1–5 Likert scale) increased from 1.96 before training ( SD = 0.74) to 3.78 six months after training ( SD = 0.70), p ˂ .001, N = 102. Following training, application of the recovery position skill ( n = 42, 46%) and application of the bleeding control skill ( n = 45, 49%) were reported by nearly half of the responding officers. Less than a quarter of officers reported applying head and neck immobilization skills ( n = 20, 22%) following training. Conclusion A PFA educational program has shown to improve police officers’ knowledge and perceived skills confidence on provision of first aid. However qualitative research need to be conducted to shed more light regarding reasons for low utilization of trained first aid skills during follow-up.
Protocol for a Randomized Controlled Trial: Peer to Peer Group Problem Management Plus (PM+) for Adult Syrian Refugees in Turkey
Authors: Uygun Ersin , Ilkkursun Zeynep , Sijbrandij Marit , Aker A. Tamer , Bryant Richard , Cuijpers Pim , Fuhr Daniela C. , de Graaff Anne M. , de Jong Joop , McDaid David , Morina Naser , Park A-La , Roberts Bayard , Ventevogel Peter , Yurtbakan Taylan , Acarturk Ceren 
Published: January 2020

Abstract
Background: A large proportion of Syrians have been exposed to potentially traumatic events, multiple losses, breakdown of supportive social networks and many of them have sought refuge in host countries where they also face post migration living difficulties such as discrimination or/and integration problems. These adversities may put Syrian refugees at high risk for common mental disorders. In response to this, the World Health Organization (WHO) developed a transdiagnostic scalable psychological intervention called Problem Management Plus (PM+) to reduce psychological distress among populations exposed to adversities. PM+ has been adapted for Syrian refugees and can be delivered by non-specialist peer lay persons in the community. Methods: A randomized controlled trial (RCT) will be conducted with 380 Syrian refugees in Turkey. After providing informed consent, participants with high levels of psychological distress (scoring >15 on the Kessler-10 Psychological Distress Scale (K10)) and functional impairment (scoring >16 on the WHO Disability Assessment Schedule 2.0 (WHODAS 2.0)) will be randomly assigned to Group PM+/Enhanced Care As Usual (Group PM+/E-CAU) (n = 190) or enhanced-care-as-usual (E-CAU) (n = 190). Outcome assessments will take place 1-week after the 5 th session (post assessment), 3 months after the 5 th session and 12 months after baseline assessment. The primary outcome is psychological distress as measured by the Hopkins Symptom Checklist (HSCL-25). Secondary outcomes include functional impairment, posttraumatic stress symptoms, self-identified problems, as well as health system and productivity costs. A process evaluation will be conducted to explore the feasibility, challenges and success of the intervention with 25 participants including participants, facilitators, policy makers and mental health professionals. Discussion: The treatment manual of the Syrian-Arabic Group PM+ and training materials will be made available through the WHO once the effectiveness and cost-effectiveness of group PM+ has been established. Keywords: Cognitive behavioural therapy, depression, anxiety, posttraumatic stress, refugee, mental health, group interventions, task shifting, trans-diagnostic
Current Status of Periodontal Disease in Adults in Takahagi, Japan:A Cross-sectional Study
Authors: Sekino Satoshi , Takahashi Ryoichi , Numabe Yukihiro , Okamoto Hiroshi 
Published: January 2020

Abstract
Background : To date, a few studies have documented the detailed periodontal conditions of a Japanese population. It is important to know if the awareness of Japanese nationals and dentists regarding oral hygiene and prevention of periodontal disease have improved when compared with the past in Japan for the development of future scenarios regarding prevention. The aim of this study was to investigate the severity, prevalence, and extent of periodontal disease in the adult population of the city of Takahagi, Japan. Results were also compared with those of an epidemiological study performed in Japan in the 1980s.
Methods : A total of 582 (aged 20 to 89 years) randomly sampled Takahagi residents answered a comprehensive questionnaire and participated in clinical examinations.
Results : The mean percentages of tooth surfaces harboring plaque and exhibiting BOP were 59.5 ± 24.9% and 31.1 ± 21.1%, respectively. The mean PPD and CAL were 2.5 ± 0.5 mm and 2.9 ± 1.0 mm, respectively. Compared with results of the 1980s survey, the mean percentages of plaque and bleeding on probing were lower in the current population. The mean CAL and prevalence of attachment loss of ³5 mm in some age groups were higher in the present study than in the 1980s study. There were no statistically significant differences with respect to mean probing depth between the 1980s and current age groups.
Conclusions : Periodontal disease was still prevalent in the current Japanese population, even though some improvement occurred. Proper public health programs therefore need to be established.
Study determinants of increased Z-Score of Body Mass Index in preschool-age children
Authors: El-Gamal Fathi Mahmoud , Babader R , Al-Shaikh M , Al-Harbi A , Al-Kaf J , Al-Kaf W 
Published: January 2020

Abstract
Objective : To determine the association between socioeconomic level, gender, stunting and other characteristics with the presence of overweight/obesity in the preschool children . Result : BMI/Age Z score > + 2 SD was found in 19.5% of the children. It was more common among the children from areas with high socio-economic level (OR: 2.43; 95% CI 1.54, 3.84, and p < 0.000) . obesity was higher among the males (OR 1.76; 95% CI 1.09, 2.8, and p < 0.02) compared to females. The increased duration of breast feeding, was significantly associated with increased BMI/Age Z-score (b= 0.027, p < 0.004). Decreased age of the child was significantly associated with increased BMI/Age Z-score (b= - 0.013, p < 0.004). The children with stunted growth were 6.7 times fold likely to have BMI/Age Z Score > + 2 SD compared to the normal children (OR 6.73; 95% CI 3.79, 10.80, and p < 0.000), after allowing for other factors. No significant association was found between allergic disorders and BMI/Age Z score > + 2 SD. Thus male gender, high socioeconomic condition, increased duration of breast feeding and stunting were significantly associated with overweight/obesity in preschool children
Build a Bioinformatics Analysis Platform and Apply it to Routine Analysis of Microbial Genomics and Comparative Genomics
Authors: Liu Hualin , Xin Bingyue , Zheng Jinshui , Zhong Hao , Yu Yun , Peng Donghai , Sun Ming 
Published: January 2020

Abstract
Genomics and comparative genomics have been increasingly used as routine methods for general microbiological researches. However, it is usually necessary to call several tools or even write some scripts to complete some simple analysis, which is complicated for most biological researchers. To simplify the operation process, especially for the convenience of microbiologists in the analysis, here we have developed PGCGAP, a comprehensive, malleable and easily-installed prokaryotic genomics and comparative genomics analysis pipeline, which implements genome assembly, gene prediction and annotation, average nucleotide identity (ANI) calculation, phylogenetic analysis, COG annotation, pan-genome analysis, inference of orthologous gene groups, variants calling and annotation and screening for antimicrobial and virulence genes. Although we have tried our best to simplify the installation and usage of PGCGAP, it may be difficult for non-bioinformatician users to master it. So, a protocol was created to help microbiologists without any experience in bioinformatics to establish their own bioinformatics platform and perform routine analysis. This protocol shows how to choose equipment, to install a Linux subsystem on a laptop with windows 10 system, to install PGCGAP and perform all analysis with an example dataset. The protocol requires a basic understanding of Linux, so an additional web page was written to help uninitiated users learn Linux and whole-genome sequencing (http://bcam.hzau.edu.cn/linuxwgs.php).
In vitro fertilization of mouse oocytes
Authors: SANKAR ADITYA , Gonzalez Javier Martin 
Published: January 2020

Abstract
this protocol describes the procedure of in vitro fertilisation of mouse oocytes. The procedure is performed at the transgenic core facility by a specialist according to the protocols found here: https://www.infrafrontier.eu/knowledgebase/protocols/cryopreservation-protocols