bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New Derivative‐Free Linear Approximation for Solving the Network Water Flow Problem With Convergence Guarantees
Authors: Wang Shen , Taha Ahmad F. , Sela Lina , Giacomoni Marcio H. , Gatsis Nikolaos 
Published: January 2020

AbstractAddressing challenges in urban water infrastructure systems, including aging infrastructure, supply uncertainty, extreme events, and security threats, depends highly on water distribution networks modeling emphasizing the importance of realistic assumptions, modeling complexities, and scalable solutions. In this study, we propose a derivative‐free, linear approximation for solving the network water flow problem. The proposed approach takes advantage of the special form of the nonlinear head loss equations, and, after the transformation of variables and constraints, the water flow problem reduces to a linear optimization problem that can be efficiently solved by modern linear solvers. Ultimately, the proposed approach amounts to solving a series of linear optimization problems. We demonstrate the proposed approach through several case studies and show that the approach can model arbitrary network topologies and various types of valves and pumps, thus providing modeling flexibility. Under mild conditions, we show that the proposed linear approximation converges. We provide sensitivity analysis and discuss in detail the current limitations of our approach and suggest solutions to overcome these. All the codes, tested networks, and results are freely available on Github for research reproducibility.
Perspectives of patients and physicians regarding hypertensive management from an online survey for excellence: a subanalysis of the PARADOX study by physician categories
Authors: Yoshida Takuo , Nishigaki Nobuhiro , Saita Shun , Shimasaki Yukio , Hasebe Naoyuki 
Published: January 2020

AbstractAn existing clinical problem in Japan is the high prevalence of uncontrolled hypertension despite the availability of various effective therapies. Here, we analyzed survey data to gain insight into this paradox from physicians’ perspectives, with results categorized according to specialty (i.e., with or without certification by the Japanese Society of Hypertension [JSH]), institution type, gender, and age. A web-based survey of typical educational activities for patients regarding hypertension management was conducted in Japan between October 19 and 31, 2017. Differences between physician groups were investigated per category. Survey results from 541 physicians were analyzed: 59 JSH certified (i.e., ‘specialist’) vs 482 non-JSH certified (i.e., ‘nonspecialist’) physicians; 192 general practitioners vs 349 hospital physicians; 500 males vs 41 females; and 178 younger (mean age: 40.7 years), 174 middle-aged (52.0 years) or 189 older (61.3 years) physicians. The most statistically significant differences between groups were observed in the category of physician specialty. Compared with nonspecialists, specialist physicians were more conscious of providing education on patient lifestyle modifications, more aware of patient- and physician-derived issues, and understood and followed the treatment guidelines. General practitioners cared more about the patient’s burden than did hospital physicians. Younger physicians identified the need to incorporate the patient’s perspective into their treatment. This analysis shows that the provision and perceptions of education differ between physician categories. Compared with specialist physicians, nonspecialists were less likely to provide adequate guidance on lifestyle modifications, possibly due to their uncertainty in understanding treatment guideline recommendations. Further education of nonspecialists on hypertension management may be warranted.
Validation of preferred salt concentration in soup based on a randomized blinded experiment in multiple regions in Japan—influence of umami (l-glutamate) on saltiness and palatability of low-salt solutions
Authors: Hayabuchi Hitomi , Morita Rieko , Ohta Masanori , Nanri Akiko , Matsumoto Hideki , Fujitani Shoji , Yoshida Shintaro , Ito Sadayoshi , Sakima Atsushi , Takase Hiroyuki , Kusaka Miho , Tsuchihashi Takuya 
Published: January 2020

AbstractSodium reduction is an important public health goal. Individual and population approaches are necessary for reducing the sodium content of processed foods and meals. The aim of the present study is to affirm the effect of monosodium l-glutamate (MSG), an umami substance, on the saltiness or palatability of low-salt solutions and to explore the preferred salt concentration in soup. Five hundred and eighty-four healthy participants from nineteen regions in Japan tasted 0.3, 0.6, and 0.9% NaCl solutions with or without 0.3% MSG. Evaluations of saltiness and palatability for each solution were conducted using a visual analog scale in a double-blinded randomized manner. Saltiness gradually increased depending on the concentration of NaCl. The saltiness of the 0.3% NaCl solution with MSG was rated significantly higher than that without MSG. The palatability ratings were higher for the solutions with MSG than for those without MSG for all NaCl concentrations. In particular, the palatability rating of the 0.3% NaCl solution with MSG was twice as high as that without MSG and was significantly higher than that of the other five test solutions. Furthermore, these results were observed to be approximately the same, irrespective of sex, age, region, etc. Salt reduction is believed to result in a loss of palatability. However, our results suggest that umami can compensate for the loss of palatability caused by salt reduction and that the addition of an appropriate amount of an umami substance can facilitate salt reduction from 0.9 to 0.3% without sacrificing palatability.
Arginine π-stacking drives binding to fibrils of the Alzheimer protein Tau
Authors: Ferrari Luca , Stucchi Riccardo , Konstantoulea Katerina , van de Kamp Gerarda , Kos Renate , Geerts Willie J. C. , van Bezouwen Laura S. , Förster Friedrich G. , Altelaar Maarten , Hoogenraad Casper C. , Rüdiger Stefan G. D. 
Published: January 2020

AbstractAggregation of the Tau protein into fibrils defines progression of neurodegenerative diseases, including Alzheimer’s Disease. The molecular basis for potentially toxic reactions of Tau aggregates is poorly understood. Here we show that π-stacking by Arginine side-chains drives protein binding to Tau fibrils. We mapped an aggregation-dependent interaction pattern of Tau. Fibrils recruit specifically aberrant interactors characterised by intrinsically disordered regions of atypical sequence features. Arginine residues are key to initiate these aberrant interactions. Crucial for scavenging is the guanidinium group of its side chain, not its charge, indicating a key role of π-stacking chemistry for driving aberrant fibril interactions. Remarkably, despite the non-hydrophobic interaction mode, the molecular chaperone Hsp90 can modulate aberrant fibril binding. Together, our data present a molecular mode of action for derailment of protein-protein interaction by neurotoxic fibrils.
Templated growth of oriented layered hybrid perovskites on 3D-like perovskites
Authors: Wang Jifei , Luo Shiqiang , Lin Yun , Chen Yifu , Deng Yehao , Li Zhimin , Meng Ke , Chen Gang , Huang Tiantian , Xiao Si , Huang Han , Zhou Conghua , Ding Liming , He Jun , Huang Jinsong , Yuan Yongbo 
Published: January 2020

AbstractThe manipulation of crystal orientation from the thermodynamic equilibrium states is desired in layered hybrid perovskite films to direct charge transport and enhance the perovskite devices performance. Here we report a templated growth mechanism of layered perovskites from 3D-like perovskites which can be a general design rule to align layered perovskites along the out-of-plane direction in films made by both spin-coating and scalable blading process. The method involves suppressing the nucleation of both layered and 3D perovskites inside the perovskite solution using additional ammonium halide salts, which forces the film formation starts from solution surface. The fast drying of solvent at liquid surface leaves 3D-like perovskites which surprisingly templates the growth of layered perovskites, enabled by the periodic corner-sharing octahedra networks on the surface of 3D-like perovskites. This discovery provides deep insights into the nucleation behavior of octahedra-array-based perovskite materials, representing a general strategy to manipulate the orientation of layered perovskites.
Chromatin mapping and single-cell immune profiling define the temporal dynamics of ibrutinib response in CLL
Authors: Rendeiro André F. , Krausgruber Thomas , Fortelny Nikolaus , Zhao Fangwen , Penz Thomas , Farlik Matthias , Schuster Linda C. , Nemc Amelie , Tasnády Szabolcs , Réti Marienn , Mátrai Zoltán , Alpár Donát , Bödör Csaba , Schmidl Christian , Bock Christoph 
Published: January 2020

AbstractThe Bruton tyrosine kinase (BTK) inhibitor ibrutinib provides effective treatment for patients with chronic lymphocytic leukemia (CLL), despite extensive heterogeneity in this disease. To define the underlining regulatory dynamics, we analyze high-resolution time courses of ibrutinib treatment in patients with CLL, combining immune-phenotyping, single-cell transcriptome profiling, and chromatin mapping. We identify a consistent regulatory program starting with a sharp decrease of NF-κB binding in CLL cells, which is followed by reduced activity of lineage-defining transcription factors, erosion of CLL cell identity, and acquisition of a quiescence-like gene signature. We observe patient-to-patient variation in the speed of execution of this program, which we exploit to predict patient-specific dynamics in the response to ibrutinib based on the pre-treatment patient samples. In aggregate, our study describes time-dependent cellular, molecular, and regulatory effects for therapeutic inhibition of B cell receptor signaling in CLL, and it establishes a broadly applicable method for epigenome/transcriptome-based treatment monitoring.
Gold clay from self-assembly of 2D microscale nanosheets
Authors: Yue Youfeng , Norikane Yasuo 
Published: January 2020

AbstractNature has always demonstrated incredible ability to create amazing materials such as soft clay which are built from nanoplatelet packing structures. It is challenging to produce artificial clays owing to the difficulty in obtaining large volume fractions of nanoplatelets and the lack of structural control in layer-by-layer packing. Here, single-crystalline Au nanosheets are synthesized by controlled growth in the bilayer membranes of succinic acid surfactants. Then, a self-assembly strategy is used to make {111}-oriented gold nanostructures at the liquid−liquid interface. The stiffness of the nanosheet assemblies are six orders of magnitude softer than bulk gold. The Au nanosheet aggregates show high plasticity and deformable into macroscale free-standing metallic architectures. They show a stress/strain-dependent conductivity owing to morphological changes. Our study provides valuable insights on the chemical synthesis of 2D nanostructures as well as for the self-assembly strategy on fabrication of mouldable metals for producing free-standing metallic architectures with microscale resolutions.
Disappearance of superconductivity due to vanishing coupling in the overdoped Bi$$_{2}$$Sr$$_{2}$$CaCu$$_{2}$$O$$_{8+\delta }$$
Authors: Valla T. , Drozdov I. K. , Gu G. D. 
Published: January 2020

AbstractIn cuprate superconductors, superconductivity is accompanied by a plethora of orders and phenomena that complicate our understanding of superconductivity in these materials. Prominent in the underdoped regime, these orders weaken or vanish with overdoping. Here, we approach the superconducting phase from the more conventional overdoped side. We present angle-resolved photoemission spectroscopy studies of Bi$$_{2}$$2Sr$$_{2}$$2CaCu$$_{2}$$2O$$_{8+\delta }$$8+δ, cleaved and annealed in ozone to increase the doping all the way to the non-superconducting phase. We show that the mass renormalization in the antinodal region of the Fermi surface that possibly reflects the pairing, weakens with doping and completely disappears precisely where superconductivity disappears. This is the evidence that in the overdoped regime, superconductivity is determined primarily by the coupling strength. A doping dependence and an abrupt disappearance above the transition temperature eliminate phononic mechanism of the observed renormalization and identify the onset of spin-fluctuations as its likely origin.
Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility
Authors: Washburn Halley R. , Xia Nan L. , Zhou Wei , Mao Yu-Ting , Dalva Matthew B. 
Published: January 2020

AbstractLocalization of the N-methyl-D-aspartate type glutamate receptor (NMDAR) to dendritic spines is essential for excitatory synaptic transmission and plasticity. Rather than remaining trapped at synaptic sites, NMDA receptors undergo constant cycling into and out of the postsynaptic density. Receptor movement is constrained by protein-protein interactions with both the intracellular and extracellular domains of the NMDAR. The role of extracellular interactions on the mobility of the NMDAR is poorly understood. Here we demonstrate that the positive surface charge of the hinge region of the N-terminal domain in the GluN1 subunit of the NMDAR is required to maintain NMDARs at dendritic spine synapses and mediates the direct extracellular interaction with a negatively charged phospho-tyrosine on the receptor tyrosine kinase EphB2. Loss of the EphB-NMDAR interaction by either mutating GluN1 or knocking down endogenous EphB2 increases NMDAR mobility. These findings begin to define a mechanism for extracellular interactions mediated by charged domains.
mTORC2-AKT signaling to ATP-citrate lyase drives brown adipogenesis and de novo lipogenesis
Authors: Martinez Calejman C. , Trefely S. , Entwisle S. W. , Luciano A. , Jung S. M. , Hsiao W. , Torres A. , Hung C. M. , Li H. , Snyder N. W. , Villén J. , Wellen K. E. , Guertin D. A. 
Published: January 2020

AbstractmTORC2 phosphorylates AKT in a hydrophobic motif site that is a biomarker of insulin sensitivity. In brown adipocytes, mTORC2 regulates glucose and lipid metabolism, however the mechanism has been unclear because downstream AKT signaling appears unaffected by mTORC2 loss. Here, by applying immunoblotting, targeted phosphoproteomics and metabolite profiling, we identify ATP-citrate lyase (ACLY) as a distinctly mTORC2-sensitive AKT substrate in brown preadipocytes. mTORC2 appears dispensable for most other AKT actions examined, indicating a previously unappreciated selectivity in mTORC2-AKT signaling. Rescue experiments suggest brown preadipocytes require the mTORC2/AKT/ACLY pathway to induce PPAR-gamma and establish the epigenetic landscape during differentiation. Evidence in mature brown adipocytes also suggests mTORC2 acts through ACLY to increase carbohydrate response element binding protein (ChREBP) activity, histone acetylation, and gluco-lipogenic gene expression. Substrate utilization studies additionally implicate mTORC2 in promoting acetyl-CoA synthesis from acetate through acetyl-CoA synthetase 2 (ACSS2). These data suggest that a principal mTORC2 action is controlling nuclear-cytoplasmic acetyl-CoA synthesis.
Ocean circulation causes the largest freshening event for 120 years in eastern subpolar North Atlantic
Authors: Holliday N. Penny , Bersch Manfred , Berx Barbara , Chafik Léon , Cunningham Stuart , Florindo-López Cristian , Hátún Hjálmar , Johns William , Josey Simon A. , Larsen Karin Margretha H. , Mulet Sandrine , Oltmanns Marilena , Reverdin Gilles , Rossby Tom , Thierry Virginie , Valdimarsson Hedinn , Yashayaev Igor 
Published: January 2020

AbstractThe Atlantic Ocean overturning circulation is important to the climate system because it carries heat and carbon northward, and from the surface to the deep ocean. The high salinity of the subpolar North Atlantic is a prerequisite for overturning circulation, and strong freshening could herald a slowdown. We show that the eastern subpolar North Atlantic underwent extreme freshening during 2012 to 2016, with a magnitude never seen before in 120 years of measurements. The cause was unusual winter wind patterns driving major changes in ocean circulation, including slowing of the North Atlantic Current and diversion of Arctic freshwater from the western boundary into the eastern basins. We find that wind-driven routing of Arctic-origin freshwater intimately links conditions on the North West Atlantic shelf and slope region with the eastern subpolar basins. This reveals the importance of atmospheric forcing of intra-basin circulation in determining the salinity of the subpolar North Atlantic.
Urban areas as hotspots for bees and pollination but not a panacea for all insects
Authors: Theodorou Panagiotis , Radzevičiūtė Rita , Lentendu Guillaume , Kahnt Belinda , Husemann Martin , Bleidorn Christoph , Settele Josef , Schweiger Oliver , Grosse Ivo , Wubet Tesfaye , Murray Tomás E. , Paxton Robert J. 
Published: January 2020

AbstractUrbanisation is an important global driver of biodiversity change, negatively impacting some species groups whilst providing opportunities for others. Yet its impact on ecosystem services is poorly investigated. Here, using a replicated experimental design, we test how Central European cities impact flying insects and the ecosystem service of pollination. City sites have lower insect species richness, particularly of Diptera and Lepidoptera, than neighbouring rural sites. In contrast, Hymenoptera, especially bees, show higher species richness and flower visitation rates in cities, where our experimentally derived measure of pollination is correspondingly higher. As well as revealing facets of biodiversity (e.g. phylogenetic diversity) that correlate well with pollination, we also find that ecotones in insect-friendly green cover surrounding both urban and rural sites boost pollination. Appropriately managed cities could enhance the conservation of Hymenoptera and thereby act as hotspots for pollination services that bees provide to wild flowers and crops grown in urban settings.
Annular flexible thermoelectric devices with integrated-module architecture
Authors: Qu Dawei , Huang Xuan , Li Xin , Wang Hanfu , Chen Guangming 
Published: January 2020

AbstractOrganic and composite thermoelectric (TE) materials have witnessed explosive developments in recent years. Design strategy of their flexible devices is vital to achieve high performance and suit various application environments. Here, we propose a design strategy of annular flexible TE devices with integrated-module architecture, where the independent modules made up of alternatively connected p-n couples are connected in series, and then rounded head-to-tail into annular configuration. The achieved devices can not only save plenty of space owing to their highly integrated structure design, but also be directly mounted on cylindrical objects (like pipes) to suit versatile applications. More importantly, the annular TE devices display excellent performances, superior to most previous work and the traditional serial single-layer film structure. For example, the annular device with eight modules consisting of three p-n couples reveals an output power of 12.37 μW at a temperature gradient of 18 K, much higher than that of the corresponding single-layer film structure (1.74 μW). The integration process is simple and easy to scale up. This architecture design strategy will greatly speed up the TE applications and benefit the research of organic and composite TE materials.
A Hyaluronan-binding Peptide (P15-1) Reduces Inflammatory and Catabolic Events in IL-1β-treated Human Articular Chondrocytes
Authors: Shortt Claire , Luyt Leonard G. , Turley Eva A. , Cowman Mary K. , Kirsch Thorsten 
Published: January 2020

AbstractInflammation plays a critical role in osteoarthritis (OA). It stimulates catabolic events in articular chondrocytes and prevents chondrogenic precursor cells from repairing cartilage lesions, leading to accelerated cartilage degradation. Therefore, the identification of novel factors that reduce catabolic events in chondrocytes and enhances chondrogenic differentiation of precursor cells in an inflammatory environment may provide novel therapeutic strategies for the treatment of OA. The goal of this study was to determine whether a hyaluronan (HA)-binding peptide (P15-1), via interacting with high molecular weight (HMW)HA can enhance the anti-inflammatory properties of HMWHA and decrease catabolic events in interleukin-1beta (IL-1β)-treated human articular chondrocytes. Treatment with P15-1 decreased catabolic events and stimulated anabolic events in articular chondrocytes cultured in an inflammatory environment. P15-1 pre-mixed with HMWHA was more effective in inhibiting catabolic events and stimulating anabolic events than P15-1 or HMWHA alone. Our findings suggest that P15-1 together with HMWHA inhibits catabolic events in articular chondrocytes via the inhibition of p38 mitogen-activated protein kinases (MAPK) and increasing the thickness of the pericellular matrix (PCM) around chondrocytes thereby decreasing catabolic signaling. Finally, conditioned medium from IL-1β and P15-1-treated human articular chondrocytes was less inhibitory for chondrogenic differentiation of precursor cells than conditioned medium from chondrocytes treated with IL-1β alone. In conclusion, P15-1 is proposed to function synergistically with HMWHA to enhance the protective microenvironment for chondrocytes and mesenchymal stem cells during inflammation and regeneration.
Associations of lymphotoxin-a (LTA) rs909253 A/G gene polymorphism, plasma level and risk of ankylosing spondylitis in a Chinese Han population
Authors: Zhu Aiping , Yang Zhicheng , Zhang Hui , Liu Ruiping 
Published: January 2020

AbstractLymphotoxin-a (LTA) may be associated with the pathogenesis of inflammatory diseases. To assess the association of the LTA rs909253 A/G polymorphism with plasma level and risk of ankylosing spondylitis (AS) in a Chinese Han population. Genotyping and LTA plasma were tested by mass spectroscopy and enzyme-linked immunosorbent assay (ELISA), respectively. The results showed that the average plasma level of LTA in AS was significantly lower than in the controls (P = 0.000). Our results also indicated that LTA rs909253 A/G was associated with a decreased risk of AS (G vs. A: P = 0.014). Significant differences were also found between the rs909253 A/G genotype and down-regulated plasma level in AS patients, compared with controls. After stratification analysis, a decreased risk of AS was associated with the LTA rs909253 G allele (G vs. A) among female patients, younger patients (Yr. < 30), HLA-B27-positive patients. In addition, In conclusion, LTA rs909253 A/G genotype has a significant relationship with decreased susceptibility to AS.