bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANTIHYPERLIPIDEMIC ACTIVITY OF ECLIPTA ALBA IN HIGH FAT DIET-INDUCED HYPERLIPIDEMIC ALBINO RATS
Authors: NAIK K SATHEESH , M GURUSHANTHAIAH , RAJU G NAGESH , S LOKANADHAM , REDDY V SESHADRI 
Published: October 2019

Objective: The objective is to study the antihyperlipidemic activity of Eclipta alba in hyperlipidemic albino rats and the use of herbal plants and their parts such as seeds, berries, leaves, roots, barks, or flowers for the development of drugs for therapeutic purposes which have an effective role in recent years.
Methods: A total of 30 adult albino rats of Wistar strain weighing 165–215 g were utilized. Animals were treated with high fat diet for 8 weeks followed by post-treatment of E. alba for 1 week, 2 weeks, and 3 weeks, respectively. After 12 h of fasting on the last day of experiment, serum blood samples were collected in EDTA vials and processed for biochemical analysis.
Results: A significant decrease in levels of total cholesterol and triglycerides was noted on animals treated with E. alba compared to high fat diet animals. Treatment of hypercholesterolemic rats with E. alba showed a marked decrease of serum low-density lipoprotein (LDL) and very LDL-cholesterol concentrations compared to the hypercholesterolemic rats. High fat diet feeding worsened the levels of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, and alkaline phosphatase enzymes, whereas the same markers were significantly improved by supplementation with E. alba compared to normal group.
Conclusion: E. alba acts as antihyperlipidemic agent in hyperlipidemic conditions and helps for better health.
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF DOLUTEGRAVIR AND RILPIVIRINE IN BULK AND PHARMACEUTICAL DOSAGE FORM AND ITS APPLICATION TO RAT PLASMA
Authors: B VEERASWAMI , VMK NAVEEN 
Published: October 2019

Objective: The present paper describes a simple, accurate, and precise reversed-phase high-performance liquid chromatography (HPLC) method for rapid and simultaneous quantification of dolutegravir (DTG) and rilpivirine (RPV) in bulk and pharmaceutical dosage form and rat plasma.
Methods: The chromatographic separation was achieved on Phenomenex C18 (150x4.6mm, 5μm). Mobile phase contained a mixture of 0.1% Ortho phosphoric acid and acetonitrile in the rato of 60:40 v/v, flow rate 1.0ml/min and ultraviolet detection at 262nm.
Results: The retention time of DTG and RPV was 4.35 min and 7.73 min, respectively. The proposed method shows a good linearity in the concentration range of 10–150 μg/ml for DTG and 5–75 μg/ml for RPV under optimized conditions. Precision and recovery study results are in between 98 and 102%. In the entire robustness conditions, percentage relative standard deviation is <2.0%. Degradation has minimum effect in stress condition and solutions are stable up to 24 h. DTG and RPV drugs are release 98% at 2 h in rat body.
Conclusion: This method is validated for different analytical performance parameters like linearity. Precision, accuracy, limit of detection, limit of quantification, robustness, and pharmacokinetic study were determined according to the International Conference of Harmonization (ICH) Q2B guidelines. All the parameters of validation were found in the acceptance range of ICH guidelines. The same method is also applied for plasma samples study in bioanalytical work.
PHYTOCHEMICAL SCREENING AND ANTIAMEBIC STUDIES OF TAMARINDUS INDICA OF LEAVES EXTRACT
Authors: H MEHDI MANSOUR ABDULNABI , YS ALARABI FADEL , FAROOQUI MAZAHAR , PRADHAN VIDYA 
Published: October 2019

Objective: The present study deals with preliminary phytochemical screening of Tamarindus indica extracts and investigates its antiamebic effect against Entamoeba histolytica in vitro.
Methods: E. histolytica was isolated from stools of patients with amebic dysentery and cultivated in lock-egg medium. The leaves extract was added to check its antiamebic activity.
Results: The phytochemical screening shows that T. indica contains alkaloids, flavonoids, phenol, and tannins. T. indica extracts (aqueous and ethanolic) were added to culture media E. histolytica. It was observed that E. histolytica count reduced to zero after 72 h and 96 h when 15 mg/ml of aqueous and ethanolic extracts were added, respectively. It is also revealed that there is no cytotoxicity against erythrocytes even when high concentration of plant leaves extract is used.
Conclusion: The in vitro sensitivity of T. indica leaves extract against the E. histolytica is established.
QUANTITATIVE PHYTOCHEMICAL SCREENING, THIN-LAYER CHROMATOGRAPHY ANALYSIS, HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINTING, AND ANTIOXIDANT ACTIVITY OF LEAVES OF DIOSPYROS MONTANA (ROXB.)
Authors: V PURI ABHIJEET 
Published: October 2019

Objective: The objective of this study was to investigate important phytochemical constituents and antioxidant potential of Diospyros montana Roxb. leaves belonging to the family Ebenaceae.
Methods: Leaves were exhaustively extracted with ethanol and fractionated into petroleum ether, chloroform, and ethyl acetate extracts. The various fractions were further analyzed for phytochemical composition and concentration-dependent antioxidant activity using conventional methods and high-performance thin-layer chromatography (HPTLC) fingerprinting. Since leaves contained phenolic compounds, extracts were evaluated for total phenolic content, flavonoids contents, and in-vitro antioxidant activity. Antioxidant potential was assessed using parameters such as superoxide radical scavenging, nitric oxide inhibition, and β-carotene/linoleic acid antioxidant activity.
Results: Primitive phytochemical investigation highlighted the presence of steroids, saponins, flavonoids, alkaloids, and tannins which were confirmed by TLC and HPTLC fingerprinting. The antioxidant activity of leaf extracts decreased in the following order ethyl acetate > ethanolic > chloroform > petroleum ether and it was comparable with standards such as ascorbic acid and butylated hydroxytoluene.
Conclusion: The present study concludes that the ethanolic extract and fractions of D. montana (Roxb.) leaves have prominent antioxidant activity comparable to standards. Therefore, D. montana (Roxb.) leaves may be used as a probable source of natural antioxidants in the pharmaceutical industry.
SOLUBILITY ENHANCEMENT OF CELECOXIB BY SOLID DISPERSION TECHNIQUE AND INCORPORATION INTO TOPICAL GEL
Authors: SHAIKH AMRIN , BHIDE PRASHANT , NACHINOLKAR REESHWA 
Published: October 2019

Objective: The aim of the present investigation was to design gels for the topical delivery of celecoxib and evaluate with an aim to increase its penetration through the skin and thereby its flux.
Method: The solubility of celecoxib is shown to be increased by preparing solid dispersions (SDs) using carriers such as mannitol, polyvinylpyrrolidone (PVP-K30), polyethylene glycol (PEG) 6000 and urea by solvent evaporation, fusion, and coevaporation methods. In vitro release profile of all SD was comparatively evaluated and studied against the pure drug. The prepared SD was subjected for percent practical yield, drug content, infrared spectroscopy, differential scanning calorimetry analysis, X-ray diffraction studies, and scanning electron microscopy (SEM) imaging. The celecoxib gel was prepared using hydroxypropyl methyl cellulose (HPMC) and Carbopol containing a permeation enhancer dimethyl sulfoxide (DMSO) at different proportions and evaluated for drug content, pH, viscosity, spreadability, extrudability, stability, and in vitro drug release.
Results: Faster dissolution rate was exhibited by SD containing 1:5 ratio of celecoxib: PVP K-30 prepared by coevaporation method. In vitro drug release of celecoxib, gels revealed that formulation with HPMC has higher drug release as compared to Carbopol.
Conclusion: The increase in dissolution rate for SD is observed in the following order of PVP K-30>urea>mannitol>PEG 6000. The CPD5 gel containing a SD CP5 and 20% DMSO showed the best in vitro release 74.13% at the end of 6 h.
ASSESSMENT OF SELF-EXTRACTED CELLULOSE FROM ORYZA SATIVA FOR DESIGN OF CONTROLLED DRUG DELIVERY SYSTEM OF DALFAMPRIDINE
Authors: JYOTHI B JEEVANA , M MOUNIKA 
Published: October 2019

Objectives: The main objective of the present work includes extraction of cellulose from Oryza sativa (OS), characterization of cellulose, development of controlled release tablet dalfampridine using cellulose of OS, and in vitro evaluation.
Methods: Dry powdered OS husk sample was extracted with a mixture of hexane and methanol (2:1, v/v) using soxhlation method and was characterized by solubility, melting point determination, differential scanning calorimetry (DSC), and Fourier-transform infrared (FTIR) analysis. Compatibility between dalfampridine and the mixture of cellulose with dalfampridine was confirmed by FTIR and DSC analysis. Then, controlled drug delivery system of dalfampridine were prepared as directly compressed tablets using various compositions containing OS cellulose, hydroxypropyl methylcellulose (HPMC), dicalcium phosphate, and magnesium stearate (8 nos. F1 to f8) and were evaluated.
Results: Cellulose was extracted from OS extract to possess its ideal characteristics. Dalfampridine and its mixture with cellulose were compatible according to FTIR and DSC analysis. Directly compressed tablets made with 10 mg of dalfampridine and OS cellulose, HPMC, dicalcium phosphate, and magnesium stearate evidenced prolonged and controlled drug delivery of dalfampridine for 12 h. Formulation made with OS cellulose 250 mg, HPMC 20 mg, dicalcium phosphate 40 mg, and magnesium stearate 5 mg was ideal without burst release.
Conclusion: Cellulose extracted from OS is used successfully for the production of directly compressed tablets of dalfampridine to elicit optimum characteristics including controlled drug delivery for 12 h.
BIOCHEMICAL SCREENING OF MARINE MICROMONOSPORA MARINA KPMS1 (MH036351) AND ITS ANTIBACTERIAL ACTIVITY AGAINST MULTIDRUG RESISTANT BACTERIA
Authors: M MOHAMED MAHROOP RAJA , A RAJA , N REEHANA 
Published: October 2019

Objective: The present investigation aimed at the screening of pharmaceutically potential antimicrobial metabolite isolated from marine Micromonospora sp. to combat the multidrug‑resistant bacteria.
Materials and Methods: The Marine sediments were randomly collected for the isolation of Micromonospora sp., from Gulf of Mannar, East Coastal Region, located at Kayalpatnam, Tuticorin district, Tamil Nadu, India. The Micromonospora sp. was cultivated by serial dilution and crowed plating method on actinomycetes isolation agar. The isolated colonies were identified by morphological, cultural, and biochemical methods. The antibacterial study of Micromonospora sp. was performed on Mueller‑Hinton agar medium against multidrug‑resistant bacteria isolated from urinary tract infection. The antibacterial compound was separated and characterized by Fourier‑transform infrared (FT‑IR) and nuclear magnetic resonance (NMR) spectrum.
Results: Based on the morphological, cultural, and biochemical studies, the isolated colonies were found to be the genera of Micromonospora. Among Micromonospora genera, Micromonospora marina KPMS1 strain showed potent antibacterial activity against multidrug‑resistant Escherichia coli, Pseudomonas aeruginosa, and Enterococcus faecalis. The FT‑IR and NMR studies showed the structural elucidation of active compounds derived from M. marina KPMS1. The 16S rRNA sequences of M. marina KPMS1 (MH036351) strain were blasted and deposited in the GenBank of National Center for Biotechnology Information.
Conclusion: The results of the study were concluded that microbial compounds are the promising sources of nearly all of the antibiotics produced by marine Micromonospora sp. The detection of new biological compounds was considered to represent a novel species of the genus Micromonospora which have been used for clinical treatment against multidrug‑resistant bacteria and were pharmaceutically important.
EVALUATION OF PHARMACOLOGICAL STABILITY OF PERILLA OIL AND PERILLA OIL CAPSULE
Authors: CHAIYASUT CHAIYAVAT , SIVAMARUTHI BHAGAVATHI SUNDARAM , KESIKA PERIYANAINA , SIRILUN SASITHORN , MAKHAMRUEANG NETNAPA , PEERAJAN SARTJIN 
Published: October 2019

Objective: The present study explained the effect of storage conditions on the stability (total phenolic content (TPC), acid and peroxide values, and antioxidant capacity) of free perilla oil (PO) and capsulated PO (POC).
Methods: PO from Mae Hong Son cultivar was used for making POC. The capsules were prepared by Nature Nutri Co., Ltd., Pathum Thani, Thailand. PO and POC samples were stored in different containers (plastic, glass, clear, and amber bottles) at various temperatures (4, 30, and 40°C) for 3 months. The physical changes (color, odor, sedimentation, and separations), TPC, and antioxidant capacity of the samples were assessed by organoleptic, Folin–Ciocalteu colorimetric, and 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid assay methods, respectively. The acid and peroxide values of the samples were also studied by the titration method.
Results: The color and odor of PO were not changed during storage, and also, there was no sedimentation and layer separation. The acid values were not significantly changed during the storage period, but the peroxide values were significantly increased in PO samples stored at 40°C while no changes were observed in POC. The TPC and antioxidant capacity of the samples were not influenced by any of the storage conditions.
Conclusion: The results suggested that POC was relatively more stable than free oil in terms of peroxidation.
INFLUENCE OF EXTRACTION PROCESS ON YIELD, TOTAL PHENOLIC CONTENT, AND ANTIOXIDANT PROPERTIES OF AVOCADO (PERSEA AMERICANA MILL.) OIL AND STABILITY ASSESSMENT
Authors: CHAIYASUT CHAIYAVAT , KESIKA PERIYANAINA , Sirilun Sasithorn , Makhamrueang Netnapa , Peerajan Sartjin , Sivamaruthi Bhagavathi Sundaram 
Published: October 2019

Objective: The present study evaluated the impact of drying and storage conditions on the quality of the avocado oil.
Methods: The fresh avocado was obtained from local farmers of Chiang Mai province, Thailand. The avocado pulp was collected and blended. The blended samples were dried at different temperatures (60, 80, and 100°C) and the oil was extracted by manual or mechanical pressing. The avocado oil samples were stored in clear and amber glass bottles at various temperatures (4, 30, and 40°C) for 3 months. The organoleptic, Folin–Ciocalteu colorimetric, 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid assay, and titration methods were used to measure the physical changes (color, odor, and precipitation), total phenolic content (TPC), antioxidant activity, and acid value (AV) and peroxide values (PVs) of the samples, respectively.
Results: The manual squeezing and mechanical pressing methods yielded about 1.17 and 24.91% of avocado oil from fresh, undried avocado flesh, and the pH of both samples was 5. The AV of manually squeezed and mechanically pressed oil samples was 2.91 and 2.88 mg KOH/g of oil, respectively. The PV of manually squeezed and mechanically pressed oil samples was 7.96 and 8.03 milliequivalent of oxygen per Kg of oil, respectively. The quality of the avocado oil dried at 60°C was high compared to other samples regarding TPC and antioxidant capacity. The storage of oil samples for 3 months at various temperatures and in different containers has not affected the quality significantly, except at 40°C.
Conclusion: The study proposed that the drying process at more than 80°C interferes with the quality of the avocado oil, and the storage conditions have a greater impact on the stability of the avocado oil. The storage at a low temperature in the absence of light may extend the shelf life of the avocado oil.
PREVALENCE OF IMPAIRMENT IN PULMONARY FUNCTION TEST IN SUGAR DISTILLERY INDUSTRY WORKERS IN KARAD TALUKA
Authors: PAWAR PRAJAKTA MAHADEO , SHINDE SANDEEP BABASAHEB 
Published: October 2019

Objective: The objective of this study was to find the prevalence of impairments in pulmonary function test (PFT) in sugar distillery industry workers in Karad taluka.
Methods: A total of 88 sugar distillery industry workers, both male and female with 5 years of the duration of exposure to sugar distillery industry in Karad taluka, were selected as per the inclusion criteria in this study. They were aged between 40 and 60 years. The outcome measures were PFT and 6 min walk test. Based on the outcome measures and conclusion were made.
Results: The study showed that among the 88 workers, 34 workers had normal pulmonary function, 25 of them had mild obstructive type of pulmonary impairments, 26 workers had mild restrictive type of pulmonary impairments, and remaining three workers had mixed type of lung disease for PFT.
Conclusion: The total 61.36% of workers were suffering from various impairments in PFT. The study concluded that statistically significant impairments in PFT are existed among workers of sugar distillery industry from Karad taluka.
PHARMACOGNOSTIC, PHYSICOCHEMICAL, AND PHYTOCHEMICAL STUDIES ON STEM BARK OF ZANTHOXYLUM ARMATUM DC.
Authors: G JOTHI , K KEERTHANA , G SRIDHARAN 
Published: October 2019

Objectives: To analyze the microscopic, macroscopic, physicochemical, and phytochemical standards of the Zanthoxylum armatum DC. (stem bark).
Methods: Pharamacognostic studies, namely macroscopic, microscopic, and powder microscopic analysis of stem bark were carried out. Physicochemical standards - ash content including total ash content, water soluble, and acid insoluble ash values, moisture content by loss on drying, solubility and extractive values of Z. armatum DC. were determined. Preliminary phytochemical screening, fluorescence analysis and quantification of alkaloids, flavonoids, phenol, tannins, saponins, and terpenoids were also studied.
Results: The macroscopic studies of stem bark showed the stem and its branches, armed with long, sharp prickles with variable size. The transverse section of bark showed the phellogen, phelloderm, cortex, phloem, and medullary rays that are characteristics to Z. armatum DC. The results of physicochemical standards give the identity and purity of the selected sample. Phytochemical studies revealed the presence of alkaloids, flavonoids, phenols, tannins, saponins, and terpenoids.
Conclusion: The present findings provide the pharmacognostic, physicochemical, and phytochemical information about the stem bark of Z. armatum and this might be useful by providing additional support with regard to its identification and standardization parameters.
ISOLATION AND IDENTIFICATION OF PHYTOSTEROLS FROM BIGNONIA VENUSTA (L.)
Authors: Sharma Manoj 
Published: October 2019

Objective: To isolate phytosterols from Bignonia venusta (L.) and its antimicrobial activity.
Introduction: B. venusta is an important medicinal plant known for its vast potential. It is a valuable plant which is commonly used in traditionalsystem of medicine for relieving pain and inflammation, as well as in a number of metabolic disorders such as diabetes and obesity.
Methods: In the present study, phytosterols from B. venusta was identified and quantified in vivo. Phytosterols were identified using chromatographic and spectral studies.
Results: Trimethyl (3,3-difluoro-2-propenyl) silane, butanoic acid, 3-methyl-3-nitroso-, methyl ester, peroxide, dibutyl, and 1,2-epoxy-5,5-dimethyl1-phenyl-3-hexyne were identified by infrared and gas chromatography-mass spectroscopy (GC-MS). GC-MS profiling showed various compounds. It is the first report on phytosterols from the experimental plant. Further, we studied antimicrobial potential of isolated compounds against clinically important microbes.
Conclusion: B. venusta (L.) is an ideal source of phytosterols and act as antimicrobial agent
SURGICAL MANAGEMENT OF FRACTURE BOTH BONES FOREARM WITH LOCKING COMPRESSION PLATE IN ADULT PATIENTS
Authors: BHARGHAVA VEM KRISHNA , KUMAR CHALLA SHIVA , MURTHY D SRINIVAS 
Published: October 2019

Objective: The objective of this study was to assess the time taken for surgery, tourniquet time, and clinical outcome in diaphyseal fractures of both forearms managed with locking compression plate (LCP).
Methods: Patients underwent open reduction and internal fixation with 3.5-mm LCP. Proximal radius was approached by dorsal Thompson incision and middle and distal radius by volar henry approach. A minimum of 6 cortices were engaged with screw fixation in each fragment. Ulna was approached directly over the subcutaneous border.
Results: Of 20 patients, 70% were men; fracture was the most common in second and third decades of life. Road traffic accident (50%) and fall (40%) were the main causes. Fractures at mid-diaphysis (70%) and transverse/short oblique (72.5%) were the most common. Closed head injury, unilateral pubic bone fracture, olecranon fracture, fracture of both bones of leg, and ipsilateral fracture shaft humerus were the associated injuries. There was no intraoperative complication. Superficial infection (n=01) and transient posterior interosseous nerve injury, in the immediate postoperative period (n=01), were the reported complications. Average time for surgery was 77 min (60–90 min) and that for tourniquet was 54 min (40–60 min). All had complete union in <6 months; 80% had healing in <4 months. 20% healed in 4–6 months. Excellent and satisfactory results were seen in 85% and 15% patients, respectively.
Conclusion: The 3.5-mm LCP, when applied properly, yields excellent outcome in fractures of forearm bones. The use of tourniquet, separate incisions for radius and ulna, and preservation of the natural curves of radius will reduce complications. Clinical outcome in terms of healing and union is excellent.
La relevancia del pensamiento de Gadamer en la filosofía: más allá de la modernidad y la posmodernidad
Authors: Velasco Gómez Ambrosio 
Published: October 2019

This paper discusses some of the main contributions of Hans-Georg Gadamer to the philosophical debate at the end of the twentieth century. Two of his contributions stand out in the paper: Gadamer's criticism of dogmatic faith in the scientific method and his criticism of a transcendental a-historical individual subject. In the elaboration of such criticisms, there is an emphasis in the reconstruction of the concept of traditions, as well as the notion of phronesis. The paper concludes that these Gadamerian notions offer an alternative way of conceiving rationality, which overcomes the dilemma between the a-historical and universalistic views that are common in modern philosophy, and the historical, but relativistic, conceptions that dominate postmodern philosophy.
Hermenéutica e historicismo
Authors: Palazón María Rosa 
Published: October 2019

The epistemologies had been based on a notion of time which registers the characters of change or recurrence of processes; in opposition to this, contemporary historicism and hermeneutics use the holistic or systemic paradigm to study contemporary differences and the deferring of processes. Gadamer supports the context-giving labor proposed by philological hermeneutics since it deals with historical change or deferring. He departs, however, from this tendency, for in his view historical interpretation is not exhausted in the context-giving labor. Gadamer's also differs from Dilthey's historicism, on the ground that the task of the historian is not to look back into the consciousness of an agent which has disappeared historically. Gadamer criticizes a version of historicism which does not take into account the historicity of the historian. That is, history is written from her present, from the effects of the historical processes which, in addition, imply the futuristic projections of the historian herself.