bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates
Authors: Ma Qiong , Zhang Qi , Yuan Youhua , Yan Wenjuan , Wang Shanmei , Xu Junhong , Zhang Jiangfeng , Wang Yuming , Li Yi 
Published: August 2020

Abstract
Background: To evaluate the accuracy and performance of the Autof MS1000 mass spectrometer in bacteria and yeast identification, 2,342 isolates were obtained from microbial cultures of clinical specimens (e.g., blood, cerebrospinal fluid, respiratory tract samples, lumbar puncture fluid, wound samples, stool, and urine) collected in 2019 in Henan Provincial People’s Hospital. Repetitive strains from the same patient were excluded. We tested the Autof MS1000 and Bruker Biotyper mass spectrometry systems and the classical biochemical identification system VITEK 2/API 20C AUX. Strains with inconsistent results between the three systems were identified by sequencing the 16S rDNA gene and others.Results: At the species level, the Autof MS1000 and Bruker Biotyper systems had isolate identification accuracies of 98.9% and 98.5%, respectively. At the genus level, the Autof MS1000 and Bruker Biotyper systems were 99.7% and 99.4% accurate, respectively. The instruments did not significantly differ in identification accuracy at either taxonomic level. The frequencies of unreliable identification were 1.1% (26/2,342) for the Autof MS1000 and 1.5% (34/2,342) for the Bruker Biotyper. In vitro experiments demonstrated that the coincidence rate of the Autof MS1000 mass spectrometer in the identification of five types of bacteria was >93%, the identification error rate was <3%, and the no identification rate was 0. This indicates that the Autof MS1000 system is an acceptable identification method.Conclusions: The Autof MS1000 mass spectrometer can be utilized to identify clinical isolates. However, an upgradation of the database is recommended to correctly identify rare strains.
The COVID-19 pandemic in Norway and Sweden – threats, trust, and impact on daily life: A comparative survey
Authors: Helsingen Lise , Refsum Erle , Gjøstein Dagrun Kyte , Løberg Magnus , Bretthauer Michael , Kalager Mette , Emilsson Louise 
Published: August 2020

Abstract
Background: Norway and Sweden have similar populations and health care systems, but different reactions to the COVID-19 pandemic. Norway closed educational institutions, and banned sports and cultural activities; Sweden kept most institutions and training facilities open. We aimed to compare peoples’ attitudes towards authorities and control measures, and perceived impact of the pandemic and implemented control measures on life in Norway and Sweden.
Methods: Anonymous web-based surveys for individuals age 15 or older distributed through Facebook using the snowball method, in Norway and Sweden from mid-March to mid-April, 2020. The survey contained questions about perceived threat of the pandemic, views on infection control measures, and impact on daily life. We performed descriptive analyses of the responses and compared the two countries.
Results: 3,508 individuals participated in the survey (Norway 3000; Sweden 508). 79% were women, the majority were 30-49 years (Norway 60%; Sweden 47%), and about 45% of the participants in both countries had more than four years of higher education.
Participants had high trust in the health services, but differed in the degree of trust in their government (High trust in Norway 17%; Sweden 37%). More Norwegians than Swedes agreed that school closure was a good measure (Norway 66%; Sweden 18%), that countries with open schools were irresponsible (Norway 65%; Sweden 23%), and that the threat from repercussions of the mitigation measures were large or very large (Norway 71%; Sweden 56%). Both countries had a high compliance with infection preventive measures (> 98%). Many lived a more sedentary life (Norway 69%; Sweden 50%) and ate more (Norway 44%; Sweden 33%) during the pandemic.
Conclusion: Sweden had more trust in the authorities, while Norwegians reported a more negative lifestyle during the pandemic. The level of trust in the health care system and self-reported compliance with preventive measures was high in both countries despite the differences in infection control measures.
CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK
Authors: Xue Jimeng , Xu Liling , Zhu Huaqun , Bai Mingxin , Li Xin , Zhao Zhen , Zhong Hua , Cheng Gong , Li Xue , Hu Fanlei , Su Yin 
Published: August 2020

Abstract
Background: Monocytes as precursors of osteoclasts in rheumatoid arthritis (RA) are well demonstrated, while monocyte subsets in osteoclast formation are still controversial. Tyro3 tyrosine kinase (Tyro3TK) is a member of the receptor tyrosine kinase family involved in immune homeostasis, the role of which in osteoclast differentiation was reported recently. This study aimed to compare the osteoclastic capacity of CD14+CD16+ and CD14+CD16- monocytes in RA and determine the potential involvement of Tyro3TK in their osteoclastogenesis.Methods: Osteoclasts were induced from CD14+CD16+ and CD14+CD16- monocyte subsets isolated from healthy control (HC) and RA patients in vitro and evaluated by tartrate-resistant acid phosphatase (TRAP) staining. Then, the expression of Tyro3TK on CD14+CD16+ and CD14+CD16- monocyte subsets in peripheral blood of RA, osteoarthritis (OA) patients, and HC were evaluated by flow cytometry and qPCR, and their correlation with RA patient clinical and immunological features was analyzed. The role of Tyro3TK in CD14+CD16- monocytes-mediated osteoclastogenesis was further investigated by osteoclast differentiation assay with Tyro3TK blockade.Results: The results revealed that CD14+CD16- monocytes were the primary source of osteoclasts. Compared with HC and OA patients, the expression of Tyro3TK on CD14+CD16- monocytes in RA patients was significantly upregulated, and positively correlated with the disease manifestations, such as IgM level, tender joint count and the disease activity score. Moreover, anti-Tyro3TK antibody could inhibit Gas6-mediated osteoclast differentiation from CD14+CD16- monocytes in a dose-dependent manner.Conclusions: These findings indicate that elevated Tyro3TK on CD14+CD16- monocytes serves as a critical signal for osteoclast differentiation in RA.
Performance test on a 5kW SOFC system under high fuel utility with practical syngas feeding
Authors: xu ming , Wang Hanlin , Liu Mingxian , Zhao Jianning , Zhang Yuqiong , Li Pingping , Shi Mingliang , Gong Siqi , Zhang Zhaohuan , Li Chufu 
Published: August 2020

Abstract
With increasing demand of green energy supply with high efficiency and low CO2 emission, Solid oxide fuel cell (SOFC) has been intensively developed in recent years. And the integration of gasification with fuel cell (IGFC) shows potential in large scale power generation to further increase the system efficiency. Reliable design of multi-stacks for large system and long term stability of stacks with practical fuel gas from industrial equipment are the key for commercial application of IGFC. In this work, a test rig of 5kW SOFC system was fabricated using practical syngas from industrial gasifiers as fuel and long term test under high fuel utility was conducted to investigate the system performance. The results show that the maximum steady output power of system is 5700W for hydrogen case and 5660W for syngas case, and the maximum steady electrical efficiency is 61.24% while the fuel utility efficiency is 89.25%. The test lasted for more than 500h as the fuel utility efficiency was larger than 83%. The performances of each stack tower are almost identical at both initial stage and after long term operation. After 500h operation, the performances of stack towers just slight decrease under lower current and almost not change under higher current. Therefore, the results illustrate that the reliability of multi-stacks design and the prospect of SOFC power generation system for further enlarging its application in a MWth demonstration.
Enrichment of apolipoprotein A-IV and apolipoprotein D in the HDL proteome is associated with HDL functions in diabetic kidney disease without dialysis
Authors: Santana Monique FM , Lira Aécio LA , Pinto Raphael , Minanni Carlos A , Silva Amanda RM , Sawada Maria IBAC , Nakandakare Edna R , Correa-Giannella Maria LC , Queiroz Marcia S , Ronsein Graziella E , Passarelli Marisa 
Published: August 2020

Abstract
Background and aims: Diabetic kidney disease (DKD) is associated with lipid derangements that worsen kidney function and enhance cardiovascular (CVD) risk. The management of dyslipidemia, hypertension and other traditional risk factors does not completely prevent CVD complications, bringing up the participation of nontraditional risk factors such as advanced glycation end products (AGEs), carbamoylation and changes in the HDL proteome and functionality. The HDL composition, proteome, chemical modification and functionality were analyzed in nondialysis subjects with DKD categorized according to the estimated glomerular filtration rate (eGFR) and urinary albumin excretion rate (AER). Methods: Individuals with DKD were divided into eGFR>60 mL/min/1.73 m2 plus AER stages A1 and A2 (n=10) and eGFR<60 plus A3 (n=25) and matched by age with control subjects (eGFR>60; n=8). Results: Targeted proteomic analyses quantified 28 proteins associated with HDL in all groups, although only 2 were more highly expressed in the eGFR<60+A3 group than in the controls: apolipoprotein D (apoD) and apoA-IV. HDL from the eGFR<60+A3 group presented higher levels of total AGEs (20%), pentosidine (6.3%) and carbamoylation (4.2 x) and a reduced ability to remove 14C-cholesterol from macrophages (33%) in comparison to HDL from controls. The antioxidant role of HDL (lag time for LDL oxidation) was similar among groups, but HDL from the eGFR<60+A3 group presented a greater ability to inhibit the secretion of IL-6 and TNF-alpha (95%) in LPS-elicited macrophages in comparison to the control group. Conclusion: The increase in apoD and apoA-IV seems to counteract the HDL chemical modification by AGEs and carbamoylation, which contributes to HDL loss of function in well-established DKD.
Utilization of Integrated Angiography-CT Interventional Radiology Suites at a Tertiary Cancer Center
Authors: Erinjeri Joseph P. , Doustaly Raphael , Avignon Grégoire , Bendet Achiude , Petre Elena , Ziv Etay , Yarmohammadi Hooman , Solomon Stephen B. 
Published: August 2020

Abstract
Background: Integrated Angiography-Computed Tomography (ACT) suites were initially designed in the 1990’s to perform complex procedures requiring high-resolution cross-sectional imaging and fluoroscopy. Since then, there have been technology developments and changes in patient management. The purpose of this study was to review the current usage patterns of a single center’s integrated ACT suites. Methods: All procedures performed in 2017 in 3 ACT suites (InterACT Discovery RT, GE Healthcare) at a tertiary cancer center were reviewed retrospectively. Usage was classified as: Standard, in which the patient underwent a single procedure using either fluoroscopy, CT, or ultrasound (US); Combined, in which the patient underwent a single procedure utilizing both fluoroscopy and CT; or Staged, in which the patient underwent 2 separate but successive procedures using fluoroscopy and CT individually. The most frequently performed Combined and Staged procedures were further reviewed to determine how the different modalities were used. The duration of the most common Staged procedures was compared to analogous procedures’ durations in single modality rooms over the period Jan 2016 to Sep 2019.Results: A total of 3591 procedures were performed on 2678 patients in the 3 ACT Suites. 80% of patients underwent a Standard procedure using fluoroscopy (38%), CT (32%) or US (10%) and accounted for 70% of the room occupation time. 14% and 3% of the patients underwent Combined or Staged procedures, occupying 19% and 5% of the room time, respectively. The remaining procedures were classified as both Combined and Staged, representing 3% of the patients and 6% of the room occupation time. The most common Combined procedures were drainages, hepatic arterial embolizations or radioembolizations, arterial, and biliary interventions. The most common Staged procedures were multiple drainages and hepatic arterial embolizations followed by biopsies or ablations. The room occupation time for liver tumor embolization and ablation was significantly shorter (p<0.01) when performed in a Staged fashion versus the analogous procedures in single modality room.Conclusion: An integrated ACT system provides the capability to perform complex Combined or Staged procedures as well as scheduling flexibility by allowing any type of case to be performed in the IR suite.
Genomic Regions Associated with Important Seed Quality Traits in Food-grade Soybeans
Authors: Whiting Rachel M. , Torabi Sepideh , Lukens Lewis , Eskandari Mehrzad 
Published: August 2020

Abstract
Background: The production of soy-based food products requires specific physical and chemical characteristics of the soybean seed. Identification of quantitative trait loci (QTL) associated with value-added traits, such as seed weight, seed protein and sucrose concentration, could accelerate the development of competitive high-protein soybean cultivars for the food-grade market through marker-assisted selection (MAS). The objectives of this study were to identify and validate QTL associated with these value-added traits in two high-protein recombinant inbred line (RIL) populations. Results: The RIL populations were derived from the high-protein cultivar ‘AC X790P’ (49% protein, dry weight basis), and two high-yielding commercial cultivars, ‘S18-R6’ (41% protein) and ‘S23-T5’ (42% protein). Fourteen large-effect QTL (R2 >10%) were identified associated with seed protein concentration. Of these QTL, seven QTL were detected in both populations, and eight of them were co-localized with QTL associated with either seed sucrose concentration or seed weight. None of the protein-related QTL was found to be associated with seed yield in either population. Sixteen candidate genes with putative roles in protein metabolism were identified within seven of these protein-related regions: qPro_Gm02-3, qPro_Gm04-4, qPro_Gm06-1, qPro_Gm06-3, qPro_Gm06-6, qPro_Gm13-4 and qPro-Gm15-3.Conclusion: The use of RIL populations derived from high-protein parents created an opportunity to identify four novel QTL that may have been masked by large-effect QTL segregating in populations developed from diverse parental cultivars. In total, we have identified nine protein QTL that were detected either in both populations in the current study or reported in other studies. These QTL may be useful in the curated selection of new soybean cultivars for optimized soy-based food products.
Association of diurnal temperature range with daily hospitalization for exacerbation of chronic respiratory diseases in 21 cities, China
Authors: Wang Zihui , Zhou Yumin , Luo Ming , Yang Huajing , Xiao Shan , Huang Xiaoliang , Ou Yubo , Zhang Yongbo , Duan Xianzhong , Hu Wei , Liao Chenghao , Zheng Yijia , Wang Long , Xie Min , Tang Longhui , Zheng Jinzhen , Liu Sha , Wu Fan , Deng Zhishan , Tian Heshen , Peng Jieqi , Wang Xinwang , Zhong Nanshan , Ran Pixin 
Published: August 2020

Abstract
Background: The association between diurnal temperature range (DTR) and hospitalization for exacerbation of chronic respiratory diseases (CRD) was rarely reported. Objectives: To examine the association between DTR and daily hospital admission for exacerbations of CRD and find out the potential effect of modifications on this association.Method: Data on daily hospitalization for exacerbation of chronic obstructive pulmonary disease (COPD), asthma and bronchiectasis and meteorology measures from 2013 through 2017 were obtained from 21 cities in South China. After controlling the effects of daily mean temperature, relative humidity (RH), particulate matter < 2.5 µm diameter (PM2.5) and other confounding factors, a standard generalized additive model (GAM) with a quasi-Poisson distribution was performed to evaluate the relationships between DTR and daily hospital admissions of CRD in a two-stage strategy. Subgroup analysis was performed to find potential modifications, including seasonality and population characteristics.Result: Elevated risk of hospitalization for exacerbation of CRD (RR=1.09 [95%CI: 1.08 to 1.11]) was associated with interquartile range (IQR) increase in DTR. The effects of DTR on hospital admissions for CRD were strong at low DTR in the hot season and high DTR in the cold season. The relative risk (RR) of hospitalization was 1.11 (95%CI: 1.08 to 1.12) for exacerbations of COPD and 1.09 (95%CI: 1.05 to 1.13) for asthma per IQR increase in DTR. The adverse effect of DTR on hospitalization for bronchiectasis was only observed in female patients (RR=1.06 [95%CI: 1.03 to 1.10]). Conclusion: Our study provided additional evidence for the association between DTR and daily hospitalization for exacerbation of CRD, and these associations are especially stronger in COPD patients and in the cold season than the hot season. Preventive measures to reduce the adverse impacts of DTR were needed for CRD patients.
Characteristics of secondary-ruptured faults in the Aso Caldera triggered by the 2016 Mw 7.0 Kumamoto earthquake
Authors: Fukushima Yo , Ishimura Daisuke 
Published: August 2020

Abstract
The 16 April 2016 Mw 7.0 Kumamoto earthquake caused prominent fault displacements and crustal deformation, not only around the main rupture faults but also around numerous secondary-ruptured faults. The physics and characteristics of such secondary faulting have not yet been studied in detail. We investigated a set of two secondary faults that appeared at the timing of the Mw 7.0 quake in the Aso Caldera by mainly using synthetic aperture radar interferometry and fault slip modeling. The two faults were found to be associated with surface displacement offsets of several centimeters or more, in the oblique sense of right-lateral and vertical motion. Fault slip inversions found that the slip was dominantly in normal sense with smaller contribution from the right-lateral component. The deeper limit of the slips was estimated to be around 1.3 km, which may coincide with the boundary between the superficial sediment layer and the basement rock. The shallowness of the slip and the difference in the dip angles of the main secondary fault and the Mw 7.0 seismogenic fault suggest separation of the two fault systems, although the fault strike and sense of motions were similar. The amount of slip on the two secondary faults was larger than that expected from the scaling law derived from seismogenic faults, which may indicate the difference in the physics of seismogenic and secondary faultings.
The segment IV approach: a useful method for achieving the critical view of safety during laparoscopic cholecystectomy in patients with anomalous bile duct
Authors: Fujioka Shuichi , Nakashima Keigo , Kitamura Hiroaki , Takano Yuki , Misawa Takeyuki , Kumagai Yu , Hata Taigo , Akiba Tadashi , Ikegami Toru , Yanaga Katsuhiko 
Published: August 2020

Abstract
Background The critical view of safety (CVS) method can be achieved by avoiding vasculo-biliary injury resulting from misidentification during laparoscopic cholecystectomy (LC). Although achieving the CVS has become popular worldwide, there is no established standardized technique to achieve the CVS in patients with an anomalous bile duct (ABD). We recently reported our original approach for securing the CVS using a new landmark, the diagonal line of the segment IV of the liver (D-line). The D-line is an imaginary line that lies on the right border of the hilar plate. The cystic structure can be securely isolated along the D-line without any misidentification, regardless of the existence of an ABD. We named this approach the segment IV approach in LC. Methods In this study, we adopted the segment IV approach in patients with an ABD. Results From October 2015 to June 2020, 209 patients underwent LC using the segment IV approach. Among them, three (1.4%) were preoperatively diagnosed with an ABD.The branching point of the cystic duct was the posterior sectional duct, anterior sectional duct, or left hepatic duct in each patient. The CVS was achieved in all cases without any complications. Conclusion It is a promising technique, especially even for patients with an ABD during LC.
A study and identification of COVID-19 viruses using N-grams with Naïve Bayes, K-Nearest Neighbors, Artificial Neural Networks, Decision tree and Support Vector Machine
Authors: Boujnouni Mohamed El 
Published: August 2020

Abstract
Coronavirus disease 2019 or COVID-19 is a global health crisis caused by a virus officially named as severe acute respiratory syndrome coronavirus 2 and well known with the acronym (SARS-CoV-2). This very contagious illness has severely impacted people and business all over the world and scientists are trying so far to discover all useful information about it, including its potential origin(s) and inter-host(s). This study is a part of this scientific inquiry and it aims to identify precisely the origin(s) of a large set of genomes of SARS-COV-2 collected from different geographic locations in all over the world. This research is performed through the combination of five powerful techniques of machine learning (Naïve Bayes, K-Nearest Neighbors, Artificial Neural Networks, Decision tree and Support Vector Machine) and a widely known tool of language modeling (N-grams). The experimental results have shown that the majority of techniques gave the same global results concerning the origin(s) and inter-host(s) of SARS-COV-2. These results demonstrated that this virus has one zoonotic source which is Pangolin.
Molecular dynamics simulation and experimental study of the surface-display of SPA protein via Lpp-OmpA system for screening of IgG
Authors: Vahed Majid , Ramezani Fatemeh , Tafakori Vida , Mirbagheri V.S. , Najafi Akram , Ahmadian Gholamreza 
Published: August 2020

Abstract
Staphylococcal protein A (SpA) is a major virulence factor of Staphylococcus aureus. S. aureus is able to escape detection by the immune system by the surface display of protein A. The SpA protein is broadly used to purify immunoglobulin G (IgG) antibodies. This study investigates the fusion ability of Lpp’-OmpA (46−159) to anchor and display five replicate domains of protein A with 295 residues length (SpA295) of S. aureus on the surface of Escherichia coli to develop a novel bioadsorbent.First, the binding between Lpp’-OmpA-SPA295 and IgGFc and the three-dimensional structure was investigated using molecular dynamics simulation. Then high IgG recovery from human serum by the surface-displayed system of Lpp’-OmpA-SPA295 performed experimentally. In silico analysiswas demonstrated the binding potential of SPA295 to IgG after expression on LPP-OmpA surface. Surface-engineered E. coli displaying SpA protein and IgG-binding assay with SDS-PAGE analysis exhibited high potential of the expressed complex on the E. coli surface for IgG capture from human serum which is applicable to conventional immune precipitation.