bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic ability of OCT parameters and retinal ganglion cells count in identification of glaucoma in myopic preperimetric eyes
Authors: Rolle Teresa , Bonetti Beatrice , Mazzucco Alberto , Dallorto Laura 
Published: August 2020

Abstract
BACKGROUND: The aim of the study is to evaluate the diagnostic ability of OCT parameters and retinal ganglion cells (RGCs) count in identify glaucomatous disease in myopic preperimetric eyes.METHODS: This was a cross-sectional observational study. The study group consisted of 154 eyes: 36 controls, 64 preperimetric (PPG), and 54 primary openangle glaucoma (POAG) eyes. Each group was divided into three subgroups based on axial length: emmetropic, myopic with axial length (AL) <25 mm, and myopic with AL>25 mm, to analyze the effect of myopia. The RGCs count was obtained using a model described later. As regard the influence of myopia on OCT parameters and RGC count, we performed Pearson’s correlation. The Area Under Receiver Operator Characteristics Curves (AUROC curves) evaluated which parameter had the best sensitivity and specificity in identifying glaucoma in myopic eyes.RESULTS: In Pearson’s test, all Ganglion Cell Complex (GCC) thicknesses showed the weakest and less significant correlation with AL in all groups. All the AUROCs were statistically significant, and above 0.5. Inferior GCC and Global Loss Volume (GLV) showed the highest AUCs in all myopic group and the best diagnostic ability in distinguishing control from glaucomatous eyes. RGCcount showed good AUROC in all groups, with sensitivities of about 83% in myopic eyes, and specificity over 91% in all groups. CONCLUSIONS: GCC is the parameter less influenced by the AL, and the inferior GCC and the GLV have the best diagnostic performance. The RGCcount has good sensitivity and specificity, so it can be used as a complementary test in the diagnosis of glaucoma in myopic preperimetric eyes.
Risk Factors for Sub–therapeutic Serum Concentrations of Magnesium Sulfate in Severe Preeclampsia of Chinese Patients
Authors: Li Jingjing , Tang Lian , Tang Ruiheng , Peng Lan , Chai Liqiang , Zhu Liping , Yu Yanxia 
Published: August 2020

Abstract
Background: Magnesium sulfate (MgSO4) is the standard drug for eclampsia prophylaxis and treatment. In China, the effective therapeutic serum magnesium level is 1.8–3.0 mmol/L. There is little information on how to achieve and maintain effective therapeutic concentrations. This study aimed to investigate risk factors for sub-therapeutic serum concentrations of MgSO4 in patients with severe preeclampsia. Methods: Patients with severe preeclampsia who received MgSO4 intravenous infusion were retrospectively reviewed. The maternal demographic characteristics, regimens for the administration of MgSO4, and lab test results of patients were collected. Multivariate logistic regression analysis and receiver operating characteristic (ROC) curve analysis were conducted for the risk factors influencing the serum magnesium concentration. Results: A total of 93 patients with severe preeclampsia were included in the study. 52 (55.91%) patients did not attain therapeutic serum magnesium levels. A multivariate logistic regression analysis identified creatinine clearance (Ccr), whether the loading dose was given, and measurement time of serum magnesium concentration (referring to the time from start of MgSO4 infusion to blood draw for serum sampling) as independent risk factors for sub-therapeutic serum magnesium concentration (P < 0.05). ROC curve analysis indicated that the continuous variable Ccr had a significant predictive value for the serum magnesium concentration, which resulted in a cutoff point of 133 mL/min; while measurement time had limited predictive value, with cutoff point of 2.375 h. Conclusions: Ccr, whether the loading dose was given, and measurement time were independent risk factors for sub-therapeutic serum magnesium concentration. A loading dose of MgSO4 everytime before the maintenance dose, as well as the duration of MgSO4 maintenance dose of more than 2.375 hours are recommended for all the patients with severe PE. Routine evaluation of serum magnesium levels is a recommended practice for women with severe PE and whose Ccr is ≥ 133 mL/min.
Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum
Authors: Jiang Heng , Zhang Nan , Ji Caihong , Meng Xiangkun , Qian Kun , Zheng Yang , Wang Jianjun 
Published: August 2020

Abstract
Background The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In this study, RNA interference (RNAi) was conducted to investigate the effects of knockdown of TcAMPKα on lipid and carbohydrate metabolism in the red flour beetle, Tribolium castaneum, and the transcriptome profiles of dsTcAMPKα-injected and dsEGFP-injected beetles under normal conditions were compared by RNA-sequencing.Results RNAi-mediated suppression of TcAMPKα increased whole-body triglyceride (TG) level and the ratio between glucose and trehalose, as was confirmed by in vivo treatment with the AMPK-activating compound, 5-Aminoimidazole-4-carboxamide1-β-D-ribofuranoside (AICAR). A total of 1184 differentially expressed genes (DEGs) were identified between dsTcAMPKα-injected and dsEGFP-injected beetles. These include genes involved in lipid and carbohydrate metabolism as well as insulin/insulin-like growth factor signaling (IIS). Real-time quantitative polymerase chain reaction analysis confirmed the differential expression of selected genes. Interestingly, metabolism-related transcription factors such as sterol regulatory element-binding protein 1 (SREBP1) and carbohydrate response element-binding protein (ChREBP) were also significantly upregulated in dsTcAMPKα-injected beetles. Conclusions AMPK plays a critical role in the regulation of beetle metabolism. The findings of DEGs involved in lipid and carbohydrate metabolism provide valuable insight into the role of AMPK signaling in the transcriptional regulation of insect metabolism.
Bioinformatic analysis reveals hub genes and pathways that promote melanoma metastasis
Authors: su wenxing , guan yi , huang biao , wang juanjuan , wei yuqian , zhao ying , Jiao qingqing , ji jiang , yu daojiang , Xu Longjiang 
Published: August 2020

Abstract
Background: Melanoma has the highest mortality rate of all skin tumors, and metastases are the major cause of death from it. The molecular mechanism leading to melanoma metastasis is currently unclear. Methods: With the goal of revealing the underlying mechanism, three data sets with accession numbers GSE8401, GSE46517 and GSE7956 were downloaded from the Gene Expression Omnibus (GEO) database. After identifying the differentially expressed gene (DEG) of primary melanoma and metastatic melanoma, three kinds of analyses were performed, namely functional annotation, protein‐protein interaction (PPI) network and module construction, and co-expression and drug-gene interaction prediction analysis. Results: A total of 41 up-regulated genes and 79 down-regulated genes was selected for subsequent analyses. Results of pathway enrichment analysis showed that extracellular matrix organization and proteoglycans in cancer are closely related to melanoma metastasis. In addition, seven pivotal genes were identified from PPI network, including CXCL8, THBS1, COL3A1, TIMP3, KIT, DCN, and IGFBP5, which have all been verified in the TCGA database and clinical specimens, but only CXCL8, THBS1 and KIT had significant differences in expression. Conclusions: To conclude, CXCL8, THBS1 and KIT may be the hub genes in the metastasis of melanoma and thus may be regarded as therapeutic targets in the future.
A comparison of the balance skills, personality, and temperament of elite sports athletes and football players
Authors: Wojciechowska-Maszkowska Bożena , Borzucka Dorota , Rogowska Aleksandra 
Published: August 2020

Abstract
Background: Although balance skills and personality have been explored in the field of sports science, little is known about the relationship between the two factors. This study aims to investigate the association of postural balance with traits of personality and temperament. The differences between elite athletes, football players, and the control sample will also be examined.Methods: There were 73 participants in the cross-sectional study, aged between 16 and 30 years (M = 21.29, SD = 2.47), including 59% men. Three samples of participants consisted of elite athletes (AE, n = 17, 23.29%) from the Polish National Team; football players (FP, n = 32, 43.84%); and the control sample (CS, n = 24, 32.88%). Postural control was assessed on a force platform (Type 9286AA, Kistler Instrumente AG, Winterthur, Switzerland) with a sampling frequency of 100 Hz. The center of pressure (COP) parameter was measured for 20 s in both conditions with eyes open, and eyes closed, in both directions, anterior‒posterior (AP) and medial‒lateral (ML). The COP parameters include standard deviation (SD), mean range (RA), mean velocity (MV), and entropy (SE). The NEO-FFI questionnaire was used to assess the Big Five personality traits, whereas the FCB-TI was used to assess temperament.Results: One-way ANOVA revealed that the EA and CS groups were more stable in the ML direction than the FP group. In the AP direction, the athletes from the EA group were more stable than the CS group. Neither personality nor temperament differentiated the three groups of participants, apart from agreeableness, which was significantly lower in both the EA and FP groups. Openness to experience is positively associated with COP parameters, as was shown by the regression analysis. All together, COP parameters can explain for about 40% of openness variability.Conclusions: The results of this study showed that EA and CS have better stability than footballers in both the ML and AP directions. Openness seems to be related to postural balance skills.
Design and validation of Brucellosis prevention questionnaire focused on animal vaccination
Authors: Bahadori Farhad , Ghofranipour Fazlollah , Ghaﬀarifar Saeideh , Ziaei Reza 
Published: August 2020

Abstract
Background: Inadequate awareness of livestock breeders concerning the transmission of brucellosis and their improper knowledge with regard to the prevention of brucellosis are among important barriers to vaccinate animals against brucellosis. The aim of this study is design and validation of a Brucellosis prevention questionnaire focused on animal vaccination . This valid questionnaire is used to design, implement and evaluate an interventional training program. Methods: In this exploratory psychometric study, a brucellosis prevention questionnaire (BPQ) was developed. Face-to-face interviews were conducted to develop the initial items of BPQ. The findings from interviews were merged with those of literature review. Face, content and construct validity of BPQ were assessed by participation of livestock breeders, veterinarians and health educationists. Impact Score (IS), Content Validity Ratio (CVR) and Content Validity Index (CVI) of the items were calculated. Construct validity of BPQ was evaluated through factor analysis. Cronbach’s alpha, Intra-class correlation coefficient (ICC), composite reliability and standard error of measurement (SEM) were measured to assess the reliability of the findings by BPQ. Results: BPQ was finalized by 53 items and its validity was confirmed by CVI, CVR, and IS of 0.90, 0.74 and 4.30, respectively. The items were loaded into three constructs of awareness, attitude and practice. The predictive power of the awareness, attitude and practice was 43.43%, 15.81% and 15.78%, respectively. The fitness of the proposed model among those constructs was confirmed by Root Mean Square Error of Approximation (RMSEA) <0.08, Normed chi-square (x 2/ df) < 5.0, Comparative Fit Index (CFI) ≥ 0.90, Tucker- Lewis Index (TLI) ≥ 0.9. Conclusion: The brucellosis prevention questionnaire has acceptable psychometric properties. Applying BPQ, veterinarians and educational planners can identify factors influencing preventive behavior of livestock breeders.
A 31-bp indel in the 5' UTR region of GNB1L is significantly associated with chicken body weight and carcass traits
Authors: Ren Tuanhui , Yang Ying , Lin Wujian , Li Wangyu , Xian Mingjian , Fu Rong , Zhang Zihao , Mo Guodong , Luo Wen , Zhang Xiquan 
Published: August 2020

Abstract
Background: G-protein subunit beta 1 like ( GNB1L ) encodes a G-protein beta-subunit-like polypeptide. Chicken GNB1L is upregulated in the breast muscle of high feed efficiency chickens, and its expression is 1.52-fold that in low feed efficiency chickens. However, no report has described the effects of GNB1L indels on the chicken carcass and growth traits. Results: This study identified a 31-bp indel in the 5' untranslated region (UTR) of GNB1L and elucidated the effect of this gene mutation on the carcass and growth traits in chickens. The 31-bp indel showed a highly significant association with the body weight at 8 different stages and was significantly correlated with daily gains at 0 to 4 weeks and 4 to 8 weeks. Similarly, the mutation was significantly associated with small intestine length, breast width, breast depth and breast muscle weight. Moreover, DD and ID were superior genotypes for chicken growth and carcass traits. Conclusions: These results show that the 31-bp indel of GNB1L significantly affects chicken body weight and carcass traits and can serve as a candidate molecular marker for chicken genetics and breeding programs.
Did Granny know best? Evaluating the Antibacterial, Antifungal and Antiviral Efficacy of Acetic Acid for Home Care Procedures.
Authors: Zinn Marc-Kevin , Bockmuehl Dirk 
Published: August 2020

Abstract
Background: Acetic acid has been used to clean and disinfect surfaces in the household for many decades. The antimicrobial efficacy of cleaning procedures can be considered particularly important for young, old, pregnant, immunocompromised people, but may also concern other groups, particularly with regards to the COVID-19 pandemics. This study aimed to show that acetic acid exhibit an antibacterial and antifungal activity when used for cleaning purposes and is able to destroy certain viruses. Furthermore, a disinfecting effect of laundry in a simulated washing cycle has been investigated.Results: At a concentration of 10% and in presence of 1.5% citric acid, acetic acid showed a reduction of >5-log steps according to the specifications of DIN EN 1040 and DIN EN 1275 for the following microorganisms: P. aeruginosa, E. coli, S. aureus, L. monocytogenes, K. pneumoniae, E. hirae and A. brasiliensis. For MRSA a logarithmic reduction of 3.19 was obtained. Tests on surfaces according to DIN EN 13697 showed a complete reduction (> 5-log steps) for P. aeruginosa, E. coli, S. aureus, E. hirae, A. brasiliensis and C. albicans at an acetic acid concentration of already 5%. Virucidal efficacy tests according to DIN EN 14476 and DIN EN 16777 showed a reduction of ≥ 4-log-steps against the Modified Vaccinia virus Ankara (MVA) for acetic acid concentrations of 5% or higher.The results suggest that acetic acid does not have a disinfecting effect on microorganisms in a dosage that is commonly used for cleaning. However, this can be achieved by increasing the concentration of acetic acid used, especially when combined with citric acid.Conclusions: Our results show a disinfecting effect of acetic acid in a concentration of 10% and in presence of 1.5% citric acid against a variety of microorganisms. A virucidal effect against enveloped viruses could also be proven. Furthermore, the results showed a considerable antimicrobial effect of acetic acid when used in domestic laundry procedures.
Prediction of Treatment Failure and Compliance in Patients with Tuberculosis
Authors: Koo Hyeon-Kyoung , Min Jinsoo , Kim Hyung Woo , Lee Joosun , Kim Ju Sang , Park Jae Seuk , Lee Sung-Soon 
Published: August 2020

Abstract
Background To improve treatment outcomes for tuberculosis (TB) , efforts to reduce treatment failure are necessary. The aim of our study was to describe the characteristics of subjects who had failed treatment of tuberculosis and identify the risk factors for treatment failure and poor compliance using national data. Methods A multicenter cross-sectional study was performed on tuberculosis subjects whose final outcome was reported as treatment failure during 2015-2017. The same number of subjects with treatment success during the same study period were randomly selected for comparison. Demographics, microbiological, radiographic, and clinical data were collected based on in-depth interviews by TB nurse specialists at all Public Private Mix (PPM) participating hospitals in South Korea. Results A total of 52 tuberculosis patients with treatment failure were enrolled. In a multivariable analysis, the presence of diabetes, previous history of tuberculosis, and cavity were identified as risk factors for treatment failure; and Medicaid support was a favorable factor for treatment success (area under the curve [AUC]: 0.79). Age, low body mass index (BMI), presence of diabetes, preexisting lung disease, positive sputum acid-fast bacilli (AFB) smear result, and the presence of multidrug-resistant tuberculosis ( MDR-TB) were significantly associated with presence of cavities. Younger age, lower BMI and previous history of TB were associated with poor compliance during treatment (AUC: 0.76). Conclusion To reduce treatment failure, careful evaluation of the presence of diabetes, previous TB history, underlying lung disease, cavity, results of sputum AFB smears, and socioeconomic status are needed. To enhance treatment compliance, more attention should be paid to younger patients with lower BMIs during follow-up.
Increased Incidence Of Susac Syndrome: A Case Series Study
Authors: Wilf-Yarkoni Adi , Elkayam O. , Aizenstein O. , Yahav O. , Furer V. , Zur D. , Goldstein M. , Barequet D. , Hallevi H. , Karni A. , Habot-Wilner Z , Regev K 
Published: August 2020

Abstract
Background Susac syndrome (SuS) is a rare condition characterized by a clinical triad of sensorineural hearing loss, branch artery occlusion and encephalopathy. This study reports an increased incidence of SuS in Israel. We describe the clinical characteristics of these patients, diagnostic procedures and the use and subsequent outcomes of newly published treatment guidelines. Methods This is a single center retrospective study. Patients who were diagnosed with SuS between July 2017 and August 2018 were enrolled in this study. Results Seven patients were diagnosed with SuS according to the diagnostic criteria in a time period of 13 months. The annual incidence was recently evaluated in Austria to be 0.024/100000, therefore, our case series represent at least a 5.4- fold increase in the annual incidence of SuS expected in Israel and a 7-fold increase in the annual incidence expected in our medical center. Mean time from the onset of the symptoms to diagnosis was three weeks and follow-up time was twenty four months. Recent exposure to cytomegalovirus was serologically evident in three patients and one patient had high titer of anti-streptolysin antibody. All patients underwent brain MRI, fluorescein angiography and audiometry. All patients were treated according to the newly recommended guidelines. All patients achieved clinical and radiological stability. Conclusions We report of an increased incidence of SuS in Israel. Infectious serological findings may imply a post infectious mechanism. The use of the recommended diagnostic procedures reduced the time to diagnosis. Newly published treatment guidelines led to favorable clinical outcomes.
Lipid metabolic gene-wide profile and survival signature of lung adenocarcinoma&nbsp;
Authors: Li Jinyou , Li Qiang , Su Zhenyu , Sun Qi , Zhao Yong , Feng Tienan , Jiang Jiayuan , Ma Haitao , Zhang Feng 
Published: August 2020

Abstract
Background: Lung cancer is the cancer with high morbidity and mortality across the globe, and lung adenocarcinoma (LUAD) is the most common histologic subtype. The disorder of lipid metabolism is related to the development of cancer. Analysis of lipid-related transcriptome helps shed light on diagnosis and prognostic biomarkers of LUAD. Methods: In this study, we performed an expression analysis of 1045 lipid metabolism-related genes between LUAD tumors and normal tissues from the Cancer Genome Atlas Lung Adenocarcinoma (TCGA-LUAD) cohort. The interaction network of differential expression genes (DEGs) was constructed to identify. The association between hub genes and overall survival was evaluated and formed a model to predict the prognosis of LUAD using a nomogram, and the model was validated by another cohort (GSE13213). Results: Finally, a total of 217 lipid metabolism-related DEGs were detected in LUAD. They were significantly enriched in Glycerophospholipid metabolism, fatty acid metabolic process, and Eicosanoid Signaling. Then we identified 6 hub genes through network and cytoHubba, including INS, LPL, HPGDS, DGAT1, UGT1A6, and CYP2C9. The high expression of CYP2C9, UGT1A6, and INS, whereas low expressions of DGAT1, HPGDS, and LPL, were associated with worse overall survival (OS) for 1925 LUAD patients. Our model found that the high-risk score group had a worse OS, and the validated cohort had the same result.Conclusion: This study constructed a signature of six lipid metabolic genes, which was significantly associated with the diagnosis and prognosis of LUAD patients. The gene signature can be used as a biomarker for LUAD in the term of lipid metabolic.