bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Analysis of the Differences in Metabolic Disorder and Atherosclerotic Cardiovascular Disease (Ascvd) Risk Between Han and Kazakh Populations in Xinjiang
Authors: Sun Yu-ping , Mayina Kahaer , Zhang Xiao-bo , Zhang Bei , Lu Ying , Song Rui-rui , Yang San-san , Yan Dandan , Hu Cheng 
Published: May 2020

Abstract
Background: Our study aimed to analyse the characteristics of glucose and lipid metabolism in a healthy Han and Kazakh population in Barkol Kazakh Autonomous County. Methods: We enrolled 4,400 subjects in Barkol Kazakh Autonomous County. The patients’ basic characteristics were recorded, chemical parameters were tested, and the characteristics of glucose and lipid metabolism between the Han and Kazakh ethnic groups were evaluated.Results: We found significant differences in glucose and lipid metabolism between Han and Kazakh people; individuals of the Han ethnicity had higher FPG and TGs but lower BMI, blood pressure, TC and LDL-C than those of the Kazakh ethnicity. Similar results remained after the participants were divided by sex. Regarding the detection rate of metabolic disorders, we observed higher rates of glucose and TG disorders but lower rates of TC, HDL-C and LDL-C disorders in individuals of the Han ethnicity than in those of the Kazakh ethnicity. For atherosclerotic cardiovascular disease (ASCVD) risk, the detection rate of an LDL-C level ≥ 4.1 mmol/L was significantly higher in Kazakh individuals than in Han individuals. However, regarding hyperglycaemic patients aged 40 years orolder with 1.8 ≤ LDL-C ≤ 4.9 mmol/L, the detection rate was higher in the Han individuals than in the Kazakh individuals. Similar results were also observed after we divided these participants by sex.Conclusion: Our study evaluated metabolic disorders in the Han and Kazakh people in Balikun County and found that metabolic disorders showed sex, ethnic and metabolite component heterogeneity.
NEMPD: A Network Embedding-Based miRNA-Protein-Disease Network Method for the miRNA-Disease Association Prediction
Authors: Ji Bo-Ya , You Zhu-Hong , Chen Zhan-Heng , Wong Leon , Yi Hai-Cheng 
Published: May 2020

Abstract
Background: As an important non-coding RNA newly discovered in recent years, MicroRNA (miRNA) plays an important role in a series of life processes and is closely associated with a variety of human diseases. Hence, the identification of potential miRNA-disease associations can make great contributions to the research and treatment of human diseases. However, to our knowledge, many of the existing state-of-the-art computational methods only utilize the single type of known association information between miRNAs and diseases to predict their potential associations, without focusing on their interactions or associations with other types of molecules.Results: In this paper, a network embedding-based the tripartite miRNA-protein-disease network (NEMPD) method was proposed for the prediction of miRNA-disease associations. Firstly, a tripartite miRNA-protein-disease network is created by integrating known miRNA-protein and protein-disease associations. Then, we utilize the network representation method-Learning Graph Representations with Global Structural Information (GraRep) to obtain the behavior information (associations with proteins in the network) of miRNAs and diseases. Secondly, the behavior information of miRNAs and diseases is combined with the attribute information of them (disease semantic similarity and miRNA sequence information) to represent miRNA-disease pairs. Thirdly, the prediction model was established based on these known miRNA-disease pairs and the Random Forest algorithm. In the results, under five-fold cross validation, the prediction accuracy, sensitivity, and AUC of NEMPD is 85.41%, 80.96%, and 91.58%. Furthermore, the performance of NEMPD was also validated by the case studies. Among the top 50 predicted disease-related miRNAs, 48 (breast neoplasms), 47 (colon neoplasms), 47 (lung neoplasms) were confirmed by two other databases.Conclusions: NEMPD has a good performance in predicting the potential associations between miRNAs and diseases and has great potency in the field of miRNA-disease association prediction in the future.
Long noncoding RNA NEAT1 regulates glioma cells proliferation and apoptosis by competitively binding with miR-324-5p and upregulating KCTD20 expression
Authors: Zhang Jiale , Li Yangyang , Liu Yuqi , Xu Guangzhi , Hei Yue , Lu Xiaoming , Weiping Liu 
Published: May 2020

Abstract
Background

Recent studies have pointed out that long non-coding RNAs (lncRNAs) play a key role in tumorigenesis, including glioma. Nuclear paraspeckle assembly transcript 1 (NEAT1), a lncRNA, has been reported to be participated in the development and progression of many types of tumors and promotes cancer cell proliferation, migration, invasion, and drug resistance. The exact role and regulatory mechanism of NEAT1 in gliomas still need to be further explored.
Methods

NEAT1 expression was detected in paired glioma tissues and adjacent normal tissues, as well as in glioma cell lines by quantitative real-time PCR (qRT-PCR). Cell viability and apoptosis were measured using flow cytometry, colony formation assays and TdT-mediated dUTP nick-end labeling (TUNEL) assay. The mechanism of competing endogenous RNA (ceRNA) between NEAT1 and miR-324-5p was determined using bioinformatics analysis, RIP and luciferase reporter assay.
Results

Here we demonstrated that lncRNA NEAT1 was upregulated and significantly associated with poor prognosis in glioma tissues. Through gain- and loss-of NEAT1 expression, we found that knockdown of NEAT1 inhibited the abilities of cell proliferation and induced G0/G1 arrest and apoptosis in vitro, suppressed tumorigenesis in vivo via sponging miR-324-5p and then upregulated KCTD20 expression. In addition, NEAT1 reversed the effects of miR-324-5p on the proliferation and apoptosis of glioma cells, and involved the inhibition of potassium channel tetramerization protein domain containing 20 (KCTD20) expression.
Conclusion

Collectively, our findings demonstrate that NEAT1 epigenetically up-regulates KCTD20 expression through competitively binding miR-324-5p, and also provides a potential therapeutic target for human glioma.
Oncogenic ZEB2/miR-637/HMGA1 signaling axis targeting vimentin promotes the malignant phenotype of glioma
Authors: Zeng Yu , Que Tianshi , Lin Jie , Xu Anqi , Wu Zhiyong , Xie Cheng , Luo Jie , Ding Shengfeng , Zhan Zhengming , Long Hao , Zhang Xian , Song Ye 
Published: May 2020

Abstract
Background

Glioma is the most common primary tumor of the central nervous system. We previously confirmed that zinc finger E-box binding homeobox (ZEB) 2 promotes the malignant progression of glioma, while microRNA-637 (miR-637) is associated with favorable prognosis in glioma. This study investigated the potential interaction between ZEB2 and miR-637 and its downstream signaling pathway in glioma.

Methods

Chromatin immunoprecipitation, luciferase reporter, and electrophoretic mobility shift assays were used to confirm ZEB2 binding to miR-637. Microarray and bioinformatic analyses were used to investigate targets of miR-637. MTT and EdU assays, transwell assays, and Boyden assays were utilized to assess cell viability, migration, and invasion, respectively. A subcutaneous xenograft model was utilized for analyzing the role of miR-637/high mobility group A1 (HMGA1) interaction in vivo. Immunohistochemistry staining of clinical samples and bioinformatic analysis of the Chinese Glioma Genome Atlas and The Cancer Genome Atlas databases were performed to further confirm the ZEB2/miR-637/HMGA1/vimentin axis.

Results

ZEB2 was bound directly bind to the E-box elements in the miR-637 promoter and promoted cell proliferation, migration, and invasion via miR-637 downregulation. Subsequent screening confirmed that HMGA1 was a direct target of miR-637, while miR-637 could propagate the malignant phenotype of glioma by suppressing HMGA1 both in vitro and in vivo. Furthermore, the interaction between cytoplasmic HMGA1 and vimentin was observed, and vimentin inhibition could abolish increased migration and invasion induced by HMGA1 overexpression. Notably, the ZEB2/miR-637/HMGA1/vimentin axis was also evident in glioma tissue samples and public glioma datasets, while both HMGA1 and vimentin were associated with an unfavorable prognosis in glioma. Additionally, upregulated HMGA1 and vimentin were found in isocitrate dehydrogenase (IDH) wild-type and 1p/19q non-codeletion diffusely infiltrating glioma.

Conclusions

We identified an oncogenic ZEB2/miR-637/HMGA1 signaling axis targeting vimentin that promotes both migration and invasion in glioma. This not only extends the current understanding of miR-637 regulation in glioma, but also unravels a novel signaling pathway that integrates a transcriptional factor, microRNA, and a chromatin remodeling factor to modulate the malignant phenotype of glioma.
CD147 promotes collective invasion through cathepsin B in hepatocellular carcinoma
Authors: Wang Shi-Jie , Chao Dong , Wei Wei , Nan Gang , Li Jia-Yue , Liu Fen-Ling , Li Ling , Jiang Jian-Li , Cui Hong-Yong , Chen Zhi-Nan 
Published: May 2020

Abstract
Background: Mounting evidence suggests that solid tumors display the features of collective invasion, however, the molecular mechanisms are far from clear. This study aims to verify the role and the underlying mechanisms of CD147 in collective invasion in hepatocellular carcinoma.Methods: Immunostaining was used to analyze human hepatocellular carcinoma specimens and three-dimensional cultures. Three-dimensional invasion model was established to mimic in vivo invasion. RNA-sequencing was used to identify downstream effectors.Results: Human hepatocellular carcinoma undergone collective invasion and CD147 was observed to be upregulated at the invasive front of tumor cell groups. CD147 was demonstrated to promote collective invasion using the modified three-dimensional invasion model, which recapitulated the main features of collective invasion. Through transcriptome analysis and enzyme activity assay, we found that CD147 enhanced cathepsin B expression and activation. Upregulated cathepsin B in hepatocellular carcinoma cells facilitated migration and invasion, which mediated CD147-induced invasive phenotype in hepatocellular carcinoma. In terms of mechanism, we found that CD147 promoted cathepsin B transcription by activating β-catenin signaling as a result of reduced GSK-3β expression. Furthermore, we found that elevated expression of CD147 as well as cathepsin B were correlated with poor prognosis in patients with hepatocellular carcinoma.Conclusions: CD147 promotes hepatocellular carcinoma cells collective invasion via upregulating cathepsin B expression and targeting CD147 would be valuable for the development of novel therapeutic modalities against invasion and metastasis of cancer.
Dietary Diversity Practice and Associated Factors Among Pregnant Women Attending Anc in Kolfe Keranyo Sub City Health Center, Addis Ababa, Ethiopia.
Authors: Brhanie Tsegahun Worku , Tefera Walelgn , Dereje Mamo 
Published: May 2020

Abstract
Background: Adequate and healthy diet during pregnancy is essential for the health of both mother and newborn. Dietary diversity is a proxy indicator of maternal nutrient adequacy. However, little is documented on dietary diversity among pregnant women. Objectives: This study was designed to assess the dietary diversity practice and associated factors among pregnant women attending ANC in health centers of the coffee keranyo sub city, Addis Ababa, Ethiopia. Methods: Institution based cross-sectional study was conducted on 406 randomly selected pregnant women attending ANC in health centers of the coffee keranyo sub city, Addis Ababa from March 2-April 2/ 2018. Data were collected by using interviewer and 24 H dietary recall method. Data had entered and analyzed using SPSS version 21. Multiple logistic regression was run to assess factors associated with the dependent variable at P<0.05. Result: The mean DDS was 5.45 +-1.83. About 60.9% of pregnant women had good dietary diversity practice. Pregnant women learned collage and above had more dietary diversity practice than the illiterate one [AOR=2.26, 95% CI: (1.066, 4.808)]. Pregnant women with monthly income more than 5,000 ETB had more dietary diversity than income less than 2,000 ETB [AOR=2.33, 95% CI: (1.234, 4.416)]. Pregnant women at second ANC visit had more dietary diversity than at the first visit [AOR=2.42, 95% CI: (1.183, 4.952)]. Having nutrition information during pregnancy increases 2 times dietary diversity practice than none informed ones [AOR=2.10, 95% CI: (1.294, 3.422)].Conclusion and Recommendation: The mean DDS among the pregnant mothers was 5.45. 60.9% of pregnant women had a good dietary diversity score and 39.1 % had poor dietary diversity. Mothers’ education, monthly income, second and third ANC visit and nutrition information had a positive significant with pregnant mothers’ dietary diversity (P<0.05. Early initiation of ANC visit and incorporation of nutrition education at each visit should be practiced. Health extension workers should provide nutritional education to every pregnant woman.
Trade-off between stochastic and deterministic processes shifts from soil to leaf microbiome of tea plant
Authors: Xu Ping , Stirling Erinne , Li Wenbing , Lv Xiaofei , Chen Haiyan , Xu Anan , Lai Wanyi , Wang Yuefei , Liu Xingmei , Ma Bin , Xu Jianming 
Published: May 2020

Abstract
Background : Plant microbiome plays an important role in promoting plant health and production. However, even though the microbiomes in various compartments have been widely investigated, the association across plant compartments from soil to leaf remain unclear. Tea is a globally popular beverage due to its flavor and health benefits associating with secondary metabolites; the microbiomes of tea plant ( Camellia sinensis ) play a significant role in the production of these secondary metabolites. Here, we sequenced the microbiomes of bulk and rhizosphere soils, roots and leaves of C. sinensis collected from tea plantations across over 2000 km to investigate the association and driving mechanisms for microbiomes in the compartments. Results : Camellia sinensis microbiomes differed between the compartments with α-diversity gradually decreasing from soils to roots and leaves. The core leaf microbiome comprised Bacilli, Sphingobacteriia and α-Proteobacteria, which we suggest might ascendingly migrate from soils to leaves. Microbial community assembly processes were dominated by deterministic processes in bulk and rhizosphere soils; these assembly processes were dominated by stochastic processes in roots and leaves. Dispersal limitation was stronger in old leaves than in other compartments. Amino acids were also critical drivers for environmental selection. The microbiomes in C. sinensis roots and leaves possessed a lower intensity of microbial associations and more negative microbial associations than in bulk and rhizosphere soils, suggesting that the contribution of microbial interactions varied in different compartments. Conclusion : In summary, there is a trade-off between stochastic and deterministic processes in microbiomes community assembly along from soil to leaf of C. sinensis . These results provide valuable information for understanding the associations and driving mechanism of microbiomes in various C. sinensis compartments, which could be used to predict C. sinensis microbiome and harness its power to improve tea production and quality.
Optimized Combination of Circulating Biomarkers as Predictors of Prognosis in AECOPD Patients Complicated with Heart Failure
Authors: Yao Can , Wang Lingwei , Shi Fei , Chen Rongchang , Li Binbin , Liu Wenwen , Feng Mengjie , Li Sinian 
Published: May 2020

Abstract
BackgroundSystematic inflammation, nutritional status, and cardiovascular function have been associated with the outcomes of acute exacerbation of chronic obstructive pulmonary disease (AECOPD) patients with heart failure (HF). However, the value of their relevant biomarkers in predicting mortality has not been well defined yet. We aimed to investigate the prognostic value of circulating biomarkers including C-reaction protein (CRP) /albumin (ALB), neutrophil–lymphocyte ratio (NLR), platelet–lymphocyte ratio (PLR), and N-terminal pro-brain natriuretic peptide (NT-proBNP) for AECOPD patients with HF.Methods A total of 146 cases of AECOPD complicated with HF were enrolled and classified into survivor group (n=94) and non-survivor group (n=52). The baseline characteristics and blood-based biomarkers were collected. The predictors for prognosis were analyzed by multivariate logistic regression, and the ability to predict 28-day mortality was evaluated by receiver operating characteristics curve (ROC) and the area under the curve (AUC).ResultsThe patients in non-survivors had significantly higher levels of CRP, CRP/ALB, NLR, PCT and NT-proBNP, but lower ALB levels compared to the survivors [145.8±110.1 VS. 66.6±70.2mg/L, 5.9±4.9 VS. 2.3±2.6, 22.2 (11.1, 40.1) VS. 12.0 (6.2, 24.8), 2.6 (0.2, 10.3) VS. 0.08 (0.1, 0.5)ng/ml, 17912.5 (9344.0, 34344.5) VS. 9809.0 (4415.9, 16387.2)ng/ml, 26.8±6.4 VS. 31.0±4.6g/L; P < 0.001, <0.001, 0.001, <0.001, <0.001, and < 0.001, respectively]. No significant difference in PLR was found between the two groups (P=0.413). The logistic analysis revealed that CRP/ALB (OR=1.303, 95%CI: 1.145-1.483, P<0.001), NT-proBNP (OR=1.041, 95%CI: 1.010-1.073, P=0.009) and NLR (OR=1.010, 95%CI: 0.999-1.022, P<0.001) are independent risk factors for predicting the 28-day mortality. The AUC of the ROC curves were 0.768, 0.767, 0.757, 0.723, 0.716, and 0.668 for CRP/ALB, PCT, CRP, NT-proBNP, ALB, and NLR, respectively. The combination of CRP/ALB, NLR and NT-proBNP as biomarkers was shown to have better accuracy for predicting prognosis (AUC=0.830, 95%CI: 0.761-0.899, P<0.001), with a higher specificity of 80.8% and specificity of 77.7% as compared with each single biomarkers.ConclusionsHigh levels of NLR, CRP/ALB and NT-proBNP may be clinical usefully predictors for death in AECOPD patients with HF. Combination of NLR with CRP/ALB and NT-proBNP can provide a higher accuracy for predicting 28-day mortality in these patients.
The Effect of Family-Centered Interactive Education Using Social Networks on the Lifestyle of Patients with Acute Coronary Syndrome: A Randomized Clinical Trial
Authors: Shojaefar Fahimeh , Dahesh Tania , Nouhi Esmat 
Published: May 2020

Abstract
Background: Patient education is a key factor in promoting the health of people with acute coronary syndrome. Families are crucial to implement educational programs. Therefore, this research aimed at determining the family-centered interactive education efficacy using social networks on the lifestyle of patients with acute coronary syndrome (ACS). Materials andMethods: The present clinical trial was conducted on 96 cases with ACS, who were randomly assigned to control and intervention groups. The former only was provided with routine training before discharge, while the latter, in addition to routine training before discharge, received education on social networking and sending text/visual messages. At baseline and three months after the intervention, the Walker’s Lifestyle Questionnaire was completed. The paired t-test was employed for data analysis.Results: the average lifestyle value of the intervention group was significantly higher compared with the control group after the intervention (P˂0.001). Moreover, the lifestyle score was significantly different pre- and post- intervention in the intervention group (P˂0.001). Conclusion: Interactive education using social networks is useful to promote the lifestyle in cases with ACS, which seems effective in planning the follow-up for these patients.
Correlation between spatial resolution and ball distortion rate of panoramic radiography
Authors: Yeom Han-Gyeol , Lee Sam-Sun , Kim Jo-Eun , Huh Kyung-Hoe , Yi Won-Jin , Heo Min-Suk , Choi Soon-Chul 
Published: May 2020

Abstract
BackgroundThe purpose of this study was to analyze the correlation between spatial resolution and ball distortion rate of panoramic radiography and to elucidate the minimum criterion for ball distortion rate, which is very relevant to clinical readability.MethodsHorizontal and vertical spatial resolution and ball distortion rates were calculated in the same position, such as the incisor, premolar, molar, and temporomandibular joint area with various object depths corresponding to 48 mm. Three devices were evaluated. A region showing spatial resolution above the reference standard was selected, and the ball distortion rate corresponding to the same region was divided into horizontal and vertical phantom groups.The mean and standard deviation of the obtained ball distortion rates were calculated. Student’s t-test was used to statistically analyze the mean difference in ball distortion rates between vertical and horizontal phantom groups.ResultsIn all devices, the horizontal line pair phantom, but not the vertical line pair phantom, was readable in all areas measured at the line pair value of at least 1.88 lp/mm. The line pair value tended to be higher toward the center and lower toward the outside. The ball distortion rate tended to decrease closer to the center and increased further away. In addition, ball distortion rates could not be measured at some areas as they were not recognized as balls due to the high degree of distortion at the outermost and innermost sides. The number of balls satisfying the reference value using the horizontal line pair phantom was 102 (mean of ball distortion rates, 20.98; standard deviation, 15.25). The number of balls satisfying the reference value using the vertical line pair phantom was 49 (mean of ball distortion rates, 16.33; standard deviation, 14.25). However, mean ball distortion rate was not significantly different between the two groups.ConclusionsFocal layer of panoramic radiography could be evaluated by the spatial resolution using horizontal and vertical line pair phantoms and by assessing ball distortion rates through a ball-type panorama phantom. A ball distortion rate of 20% could be used as a threshold to evaluate the focal layer of panoramic radiography.