bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of sex, socio‐economic status, and remoteness on therapy and survival in heart failure
Authors: Gutman Sarah J. , Costello Ben T. , Papapostolou Stavroula , Iles Leah , Ja Johnson , Hare James L. , Ellims Andris , Marwick Thomas H. , Taylor Andrew J. 
Published: October 2019

AbstractAimsThis study aims to determine if traditional markers of disadvantage [female sex, low socio‐economic status (SES), and remoteness] are associated with lower prescription of evidence‐based therapy and higher mortality among patients with moderate–severe heart failure with reduced ejection fraction.Methods and resultsWe recruited 452 consecutive class II–III heart failure with reduced ejection fraction patients. Baseline clinical data were recorded prospectively. The primary outcome was the association of female sex on overall survival. Secondary outcomes included association between evidence‐based therapy delivery and sex and association of SES and remoteness on heart failure therapy and survival. The Australian Bureau of Statistics generated all indices. Median follow‐up was 37.9 months. One hundred and nine patients (24.3%) were women. There was no difference in overall survival based on sex (hazard ratio = 1.19, 95% confidence interval: 0.74–1.92, 0.48). There was no difference in prescription of beta‐blockers [χ2(1) = 0.91, 0.66], angiotensin‐converting enzyme inhibitors [χ2(1) = 0.001, 0.97], nor aldosterone antagonists [χ2(1) = 2.71, 0.10]. There was no difference in rates of primary prevention implantable cardioverter‐defibrillator implantation in men compared with women [χ2(1) = 0.35, 0.56]. Neither higher SES nor inner city residence conferred an overall survival benefit.ConclusionsIn this Australian cohort of heart failure patients, delivery of care and likelihood of death are comparable between the sexes, SES groups, and rural vs. city residents.
Polyaniline Sheathed Black Phosphorous: A Novel, Advanced Platform for Electrochemical Sensing Applications
Authors: Durai L. , Gopalakrishnan A. , Vishnu Nandimalla , Badhulika Sushmee 
Published: October 2019

AbstractDespite its excellent properties, the inherent unstable nature of black phosphorus (BP) in ambient atmosphere has severely restricted its use in electrochemical sensing applications. In this work, polyaniline (PANI) sheathed BP was prepared via the electrochemical polymerisation of aniline on BP coated screen printed carbon electrode (i. e., SPCE/BP) which resulted in an efficient, stable electrochemical platform (i. e., SPCE/BP@PANI) with improved properties which was evaluated for electrochemical detection of two model bioanalytes namely, ascorbic acid (AA) and Hydrazine (Hy). The formation of PANI on the SPCE/BP exhibited a pair of stable and well‐defined redox peaks indicating the better adsorption energy and fast electron transfer nature of BP as compared to other 2D materials like graphene and transitional metal dichalcogenides. FESEM and XPS studies revealed the formation and uniform growth of PANI on BP surface without any aggregation. Electrochemical impedance spectroscopy analyses revealed that SPCE/BP@PANI can act as a suitable electrocatalyst material for the sensing of AA and Hy. Thus, SPCE/BP@PANI electrode exhibited low limit of detection (DL; 1.69 μM), excellent reproducibility and better selectivity towards AA oxidation over glucose, sucrose, urea, citric acid, sodium, nitrate, nitrite and magnesium with a sensitivity of 3.38 A M−1 cm−2 (R2=0.98) in the dynamic range of 10–1100 μM. The excellent analytical performance of the BP@PANI is plausible due to better adsorption energy and fast electron transfer of BP. Further, SPCE/BP@PANI was also used for successful detection of AA in processed fruit juice with good recovery. Under the optimal DPV conditions, the modified electrode was extended for detection of Hy in a linear range of 100–1500 μM with sensitivity of 0.09 A M−1 cm−2 (R2=0.99) and DL=89 μM validating the potential of BP based composites in wide range of electrochemical applications.
Individual differences in pain sensitivity are associated with cognitive network functional connectivity following one night of experimental sleep disruption
Authors: Letzen Janelle E. , Remeniuk Bethany , Smith Michael T. , Irwin Michael R. , Finan Patrick H. , Seminowicz David A. 
Published: October 2019

AbstractPrevious work suggests that sleep disruption can contribute to poor pain modulation. Here, we used experimental sleep disruption to examine the relationship between sleep disruption‐induced pain sensitivity and functional connectivity (FC) of cognitive networks contributing to pain modulation. Nineteen healthy individuals underwent two counterbalanced experimental sleep conditions for one night each: uninterrupted sleep versus sleep disruption. Following each condition, participants completed functional MRI including a simple motor task and a noxious thermal stimulation task. Pain ratings and stimulus temperatures from the latter task were combined to calculate a pain sensitivity change score following sleep disruption. This change score was used as a predictor of simple motor task FC changes using bilateral executive control networks (RECN, LECN) and the default mode network (DMN) masks as seed regions of interest (ROIs). Increased pain sensitivity after sleep disruption was positively associated with increased RECN FC to ROIs within the DMN and LECN (F(4,14) = 25.28, pFDR = 0.05). However, this pain sensitivity change score did not predict FC changes using LECN and DMN masks as seeds (pFDR > 0.05). Given that only RECN FC was associated with sleep loss‐induced hyperalgesia, findings suggest that cognitive networks only partially contribute to the sleep‐pain dyad.
Age affects the contribution of ipsilateral brain regions to movement kinematics
Authors: Tscherpel Caroline , Hensel Lukas , Lemberg Katharina , Freytag Jana , Michely Jochen , Volz Lukas J. , Fink Gereon R. , Grefkes Christian 
Published: October 2019

AbstractHealthy aging is accompanied by changes in brain activation patterns in the motor system. In older subjects, unilateral hand movements typically rely on increased recruitment of ipsilateral frontoparietal areas. While the two central concepts of aging‐related brain activity changes, “Hemispheric Asymmetry Reduction in Older Adults” (HAROLD), and “Posterior to Anterior Shift in Aging” (PASA), have initially been suggested in the context of cognitive tasks and were attributed to compensation, current knowledge regarding the functional significance of increased motor system activity remains scarce. We, therefore, used online interference transcranial magnetic stimulation in young and older subjects to investigate the role of key regions of the ipsilateral frontoparietal cortex, that is, (a) primary motor cortex (M1), (b) dorsal premotor cortex (dPMC), and (c) anterior intraparietal sulcus (IPS) in the control of hand movements of different motor demands. Our data suggest a change of the functional roles of ipsilateral brain areas in healthy age with a reduced relevance of ipsilateral M1 and a shift of importance toward dPMC for repetitive high‐frequency movements. These results support the notion that mechanisms conceptualized in the models of “PASA” and “HAROLD” also apply to the motor system.
Utility of negative‐pressure wound therapy for orocutaneous and pharyngocutaneous fistula following head and neck surgery
Authors: Inatomi Yusuke , Kadota Hideki , Yoshida Sei , Kamizono Kenichi , Shimamoto Ryo , Fukushima Seita , Miyashita Kayo , Matsuo Mioko , Yasumatsu Ryuji , Tanaka Shunichiro , Fukushima Junichi 
Published: October 2019

AbstractBackgroundBecause of the difficulty of airtight sealing and risk of salivary contamination, negative‐pressure wound therapy (NPWT) has rarely been applied for postoperative fistula following head and neck surgery; thus, its utility remains unclear.MethodsWe applied NPWT in 34 patients who developed orocutaneous and pharyngocutaneous fistula after head and neck surgery. Here we retrospectively analyzed the utility of NPWT for managing those fistulas.ResultsThirty‐two patients (94.1%) underwent NPWT as scheduled without adverse events. In 28 patients (82.4%), fistula closure was completed only by NPWT, and the mean period to fistula closure was 30.4 days. The mean period to closure did not differ significantly between fistulas with (21.7 days) and without (39.1 days) previous irradiation.ConclusionsAirtight sealing can be maintained and postoperative fistula can be closed by NPWT with a high success rate, even after previous irradiation. NPWT is an effective and minimally invasive treatment for postoperative fistula.
Learning, memory and consolidation mechanisms for behavioral control in hierarchically organized cortico‐basal ganglia systems
Authors: Rusu Silviu I. , Pennartz Cyriel M. A. 
Published: October 2019

AbstractThis article aims to provide a synthesis on the question how brain structures cooperate to accomplish hierarchically organized behaviors, characterized by low‐level, habitual routines nested in larger sequences of planned, goal‐directed behavior. The functioning of a connected set of brain structures—prefrontal cortex, hippocampus, striatum, and dopaminergic mesencephalon—is reviewed in relation to two important distinctions: (a) goal‐directed as opposed to habitual behavior and (b) model‐based and model‐free learning. Recent evidence indicates that the orbitomedial prefrontal cortices not only subserve goal‐directed behavior and model‐based learning, but also code the “landscape” (task space) of behaviorally relevant variables. While the hippocampus stands out for its role in coding and memorizing world state representations, it is argued to function in model‐based learning but is not required for coding of action–outcome contingencies, illustrating that goal‐directed behavior is not congruent with model‐based learning. While the dorsolateral and dorsomedial striatum largely conform to the dichotomy between habitual versus goal‐directed behavior, ventral striatal functions go beyond this distinction. Next, we contextualize findings on coding of reward‐prediction errors by ventral tegmental dopamine neurons to suggest a broader role of mesencephalic dopamine cells, viz. in behavioral reactivity and signaling unexpected sensory changes. We hypothesize that goal‐directed behavior is hierarchically organized in interconnected cortico‐basal ganglia loops, where a limbic‐affective prefrontal‐ventral striatal loop controls action selection in a dorsomedial prefrontal–striatal loop, which in turn regulates activity in sensorimotor‐dorsolateral striatal circuits. This structure for behavioral organization requires alignment with mechanisms for memory formation and consolidation. We propose that frontal corticothalamic circuits form a high‐level loop for memory processing that initiates and temporally organizes nested activities in lower‐level loops, including the hippocampus and the ripple‐associated replay it generates. The evidence on hierarchically organized behavior converges with that on consolidation mechanisms in suggesting a frontal‐to‐caudal directionality in processing control.
Association between dietary inflammatory index and risk of cardiovascular disease in the Mashhad stroke and heart atherosclerotic disorder study population
Authors: Asadi Zahra , Yaghooti‐Khorasani Mahdiyeh , Ghazizadeh Hamideh , Sadabadi Fatemeh , Mosa‐Farkhany Ehsan , Darroudi Susan , Shabani Niloofar , Kamel‐khodabandeh Atiyeh , Bahrami Afsane , Khorrami‐Mohebbseraj Mohammad‐Sadegh , Heidari‐Bakavoli Sahar , Heidari‐Bakavoli Alireza , Esmaily Habibollah , Moohebati Mohsen , Oladi Mohammad R. , Shivappa Nitin , Hébert James R. , Ferns Gordon A. , Ghayour‐Mobarhan Majid 
Published: October 2019

AbstractThe dietary inflammatory index (DII) is a novel way of describing diet that has been studied in relation to various health conditions, including cardiovascular disease (CVD) in several populations. We aimed to investigate the association between DII and CVD events among a representative population sample in northeastern Iran. This prospective cohort study was a subsample of 4,672 adults aged 35–65 years, and recruited as part of Mashhad stroke and heart atherosclerotic disorder cohort study population. The DII was computed at baseline according to a 65‐item validated food frequency questionnaire. Cox regression was used to determine the association of DII with incident CVD. One hundred twenty‐four participants developed CVD (including 24 cases of myocardial infarction [MI], 34 cases of stable angina [SA], and 66 cases of unstable angina [UA]). After adjusting for potential confounding factors, a hazard ratios of 1.06 (95% confidence interval: 0.70–1.60), 1.36 (95% confidence interval: 0.52–3.52), 1.33 (95% confidence interval: 0.60–2.94), and 0.86 (95% confidence interval: 0.48–1.53) were found for total CVD, MI, SA, and UA events, respectively, among the participants with proinflammatory diet (DII ≥ 0) versus those with anti‐inflammatory diet (DII < 0). There was no statistically significant association between the DII and total CVD, MI, SA, or UA in this population of middle‐aged Iranian men and women.
Acellular polycaprolactone scaffolds laden with fibroblast/endothelial cell‐derived extracellular matrix for bone regeneration
Authors: Junka Radoslaw , Quevada Kristian , Yu Xiaojun 
Published: October 2019

AbstractInconsistencies in graft osteoconduction and osteoinduction present a clinical challenge in regeneration of large bone defects. Deposition of decellularized extracellular matrix (dECM) on tissue engineered scaffolds offers an alternative approach that can enhance these properties by mimicking bone's molecular complexity and direct infiltrating cells to repair damaged bone. However, dECMs derived from homogenous cell populations do not adequately simulate the heterogeneous and vascularized microenvironment of the bone. In this study, successive culture and decellularization of fibroblasts and endothelial cells (ECs) grown on polycaprolactone microfibers was used to develop a bioactive scaffold with heterogeneous dECM mimicking endothelial basement membrane. These scaffolds had greater amount of protein and minimally increased nucleic acid content than scaffolds with homogenous culture dECM. Coomassie Blue and antibody staining revealed extensive tube formation by ECs on fibroblast dECM. Fibroblast/endothelial dECM significantly enhanced osteoblast attachment, alkaline phosphatase activity, and osteocalcin‐ and osteopontin‐positive extracellular mineral deposits. We demonstrated that the osteoconduction of dECMs can be tailored with the appropriate combination of cells to accelerate osteoblast mineral secretion. The overall concept can be expanded to generate increasingly more complex tissue constructs for regeneration of bone defects and other vascularized tissues.
The influence of ultrafine‐grained structure on the mechanical properties and biocompatibility of austenitic stainless steels
Authors: Rybalchenko Olga V. , Anisimova Natalia Yu. , Kiselevsky Mikhail V. , Belyakov Andrey N. , Tokar Aleksei A. , Terent'ev Vladimir F. , Prosvirnin Dmitry V. , Rybalchenko Georgy V. , Raab Georgi I. , Dobatkin Sergey V. 
Published: October 2019

AbstractIn this study, equal‐channel angular pressing (ECAP) of austenitic 316L and Cr–Ni–Ti stainless steels was carried out. Effect of ECAP at 400°C on the evolution of the microstructure, mechanical properties, and biocompatibility of these steels was investigated. The biocompatibility of samples with the ultrafine grain structure obtained in the ECAP process did not deteriorate in comparison with an austenitic 316L stainless steel in coarse‐grained state. However, this treatment enhances the multipotent mesenchymal stromal/stem cell proliferation by 26% for 316L steel and by 17% for Cr–Ni–Ti stainless steel in comparison with coarse‐grained counterparts. At the same time, ECAP contributes to a significant improvement in performance and weight reduction of medical devices, which is especially important for the creation of implanted prostheses for replacement of skeletal defects, due to significant increase in specific strength of steels. The strength properties of austenitic stainless steels were remarkably improved due to the grain refinement and deformation twinning resulted from ECAP at 400°C. After ECAP, the yield strength of 316L and Cr–Ni–Ti stainless steels increased by 4.2 and 2.9 times up to 950 and 900 MPa, and the fatigue limit by 2 and 1.7 times up to 500 and 475 MPa, respectively, comparing to coarse‐grained counterparts.
Self‐protective transcriptional alterations in ZF4 cells exposed to Pb(NO3)2 and AgNO3
Authors: Liu Guangxing , Tian Jingjing , Yin Huancai , Yin Jian , Tang Yuguo 
Published: October 2019

AbstractIn this study, gene expression alterations of phase I to III enzymes, transcription factors, and microRNA (miRNA) in embryonic zebrafish fibroblasts (ZF4) cells after the treatment of Pb(NO3)2 and AgNO3 were investigated, to illustrate the possible detoxification pathway of heavy metal ions. It was observed that both metals caused concentration‐dependent death and moderate elevation of oxidative stress in ZF4 cells. In response to such toxicity, upregulation of multidrug resistance protein (mdr)4 and multiresistance‐associated protein (mrp)1 were found. However, enhanced expression of glutathione S‐transferase (gst) and cytochrome P450 (cyp)1a could only be detected during the exposure of Pb2+. In addition, both metals induced extensive upregulation of pregnane X receptor (pxr), but only moderate elevation of E2‐related factor (nrf2), while they suppressed the expression of miR‐122 and miR‐126. In conclusion, Pb2+ and Ag+ shared the same detoxification mechanism including ABC transporters, Pxr, and miRNA in ZF4 cells, which needs further investigation.
Elevated serum procalcitonin level in patients with chronic kidney disease without infection: A case‐control study
Authors: Wu Sen‐Chao , Liang Cai‐Xia , Zhang Yan‐Lin , Hu Wei‐Ping 
Published: October 2019

AbstractBackgroundInflammation is a necessary component of chronic kidney disease (CKD) that can be attributed to an accumulation of toxins and a reduced clearance of proinflammatory cytokines. Procalcitonin (PCT) is a widely applied biomarker in the diagnosis of infection, and considering the presence of pre‐existing inflammation in CKD patients, the PCT level could be high in such a population; however, no reference value for PCT in CKD patients has been available to date.MethodsDuring the present study period, 361 CKD patients and 119 healthy controls were included. The PCT level and other biochemistry parameters were assayed by using a COBAS system. Statistical analysis was conducted to compare the differences in PCT levels and other biochemistry parameters between the two groups, and linear regression was used to assess the correlation between two variables. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the performance of PCT and the optimal cutoff value to differentiate between CKD patients and healthy controls.ResultsThe PCT level in CKD patients was significantly higher than that in healthy controls, and among the CKD patients, the PCT level was increased with advanced clinical stage. Moreover, PCT was moderately correlated with CysC. The optimal off‐value was 0.075 with a sensitivity of 94.7% and specificity of 90.8%.ConclusionThe PCT level was significantly higher in CKD patients than in healthy controls, and the reference value for CKD patients should be adjusted to avoid unnecessary antibiotic treatments which may pose a negative impact on residual renal function.
circRNA‐associated ceRNA network construction reveals the circRNAs involved in the progression and prognosis of breast cancer
Authors: Ma Xiaoran , Liu Cun , Gao Chundi , Li Jie , Zhuang Jing , Liu Lijuan , Li Huayao , Wang Xue , Zhang Xiaoming , Dong Shixia , Zhou Chao , Sun Changgang 
Published: October 2019

AbstractRecently, increasing evidences show that circular RNAs (circRNAs) are important regulators of various diseases, especially cancer. However, the regulatory role and the potential mechanism of action of circRNAs in breast cancer remain largely unknown. In this study, weighted gene co‐expression network analysis was conducted with the differentially expressed miRNAs and mRNAs in breast cancer from The Cancer Genome Atlas database to identify the key modules associated with the carcinogenesis of breast cancer. In the significant turquoise and brown modules, 22 miRNAs and 1877 mRNAs were identified, respectively. Then, We compared and predicted the target genes and performed survival analysis to identify the miRNAs and mRNAs related to the prognosis of breast cancer. A circRNA‐related competitive endogenous RNA network was identified by database co‐screening, and deleted in liver cancer 1 (DLC1) was identified as a key gene. Finally, to assess how genes in key modules and key genes contribute to the development of breast cancer, relevant pathway information was obtained through DAVID and Gene Set Enrichment Analysis. These data demonstrated that three circRNAs (hsa‐circ‐0083373, hsa‐circ‐0083374, and hsa‐circ‐0083375) that regulate DLC1 expression via hsa‐mir‐511 and are involved in the pathogenesis and development of breast cancer.
Comparative anatomy on the vegetative organs of genus Ziziphora L. (Lamiaceae) from Turkey
Authors: Selvi Selami , Satil Fatih 
Published: October 2019

AbstractThe genus Ziziphora L. (Lamiaceae) is represented by five species (nine taxa) in the Turkish Flora. These taxa are Z. clinopodioides Lam. (subsp. elbursensis, subsp. filicaulis, subsp. kurdica, subsp. rigida), Z. capitata L., Z. persica Bunge, Z. tenuior L., Z. taurica Bieb. subsp. taurica, and Z. taurica Bieb. subsp. cleonioides (Boiss.) Davis which to be an endemic taxon for Turkey. They are strongly aromatic herbs which contain rich pulegone and used as herbal teas and spices and for this reason. In this study, comparative anatomy of the genus Ziziphora growing in Turkey is presented for the first time. In anatomical studies, cross sections of vegetative organs such as the root, stem, and leaf (lamina and petiole) were examined. In addition, to exhibit stomatal distribution and anatomy on adaxial and abaxial leaves were taken surface sections of the lamina and calculated stomatal index. Lamina and petiole anatomy were shown to be of great importance in the taxonomy of the Ziziphora taxa. The presence or absence of sclerenchyma in midrib of lamina and petiole, cortex parenchyma layer, mesophyll structure, and epidermal surface were found to be important characters for identification of Ziziphora taxa.
Protein‐losing gastroenteropathy with severe hypoalbuminemia associated with Sjögren’s syndrome: A case report and review of the literature
Authors: Akaishi Tetsuya , Yasaka Ken , Abe Michiaki , Fujii Hiroshi , Watanabe Mika , Shirai Tsuyoshi , Ishizawa Kota , Takayama Shin , Kagaya Yutaka , Harigae Hideo , Ishii Tadashi 
Published: October 2019

AbstractA 30‐year‐old man with severe hypoalbuminemia (serum albumin: 0.9 g/dL) was admitted with severe bilateral leg edema and unilateral pleural effusion. Serum anti‐SS‐A and SS‐B antibody levels were abnormally elevated, and his symptoms fulfilled the diagnostic criteria for Sjögren's syndrome. Technetium‐99m albumin scintigraphy revealed protein leakage from a large area of the small intestine. Immunohistochemistry revealed perivascular deposition of C1q, C3d, and immunoglobulin G in the duodenal mucosa. The patient was diagnosed with protein‐losing gastroenteropathy associated with Sjögren's syndrome. Within 2 months of treatment with oral prednisolone and mycophenolate mofetil, the clinical symptoms of hypoalbuminemia and Sjögren's syndrome disappeared completely.