bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tibiofemoral rotation alignment in the normal knee joints among Chinese adults: A CT analysis
Authors: Lu Yufeng , Ren Xiaoyu , Liu Benyin , Xu Peng , Hao Yangquan 
Published: April 2020

Abstract
Background: Consensus on tibial rotation in total knee arthroplasty (TKA) remains controversial. The present study aimed to investigate the closest anatomical reference to surgical epicondylar axis (SEA) among 10 tibial markers in Chinese adults.Methods: This study included examination of 122 normal lower extremities. Briefly, 10 axes were drawn on the axial sections: transverse axis of tibia (TAT), axis of medial edge of patellar tendon (MEPT), axis of medial 1⁄3 of patellar tendon attachment (M1/3), Akagi line, Insall line, axis of medial border of tibial tubercle (MBTT), and axis of anterior border of the tibia 1-4 (ATC1-4). The mean angles between TAT and SEA and that between other axes and the line perpendicular to SEA were measured. Pairwise differences among the 10 tibial axes were examined by applying one-way analysis of variance (ANOVA) and paired t-test.Results: In all the knees, the mean angles of M1/3, Akagi line, Insall line, MBTT, ATC1, ATC2, ATC3, and ATC4 axes were compared to the line perpendicular to the projected SEA and found to be 10.2 ± 5.1°, 1.4 ± 5.0°, 11.9 ± 5.4°, 3.6 ± 4.8°, 12.0 ± 6.9°, 7.2 ± 8.6°, 7.1 ± 10.4°, and 6.6 ± 13.5° external rotation, respectively, and the MEPT axis was 1.6 ± 4.5° internal rotation. The mean angle for TAT was 4.1 ± 5.3° external rotation. The M1⁄3 and Insall line were significantly more externally rotated than Akagi line, MEPT, MBTT, TAT, ATC2, ATC3, and ATC4 axes. No significant differences were noted between the TAT axis and the MBTT axis and among the ATC2, ATC3, and ATC4 axes.Conclusion: The Akagi line, MBTT, and TAT showed good consistency with SEA in the axial femorotibial alignment with knee in extension. The middle segment of the anterior tibial crest also demonstrated good alignment consistency with SEA for the axial femorotibial alignment. Hence, these markers can be used as reliable references for rotational alignment of the tibial component in TKA.
Markers for monitoring hydrogen peroxide resistance in the salmon louse, Lepeophtheirus salmonis
Authors: Agusti-Ridaura Celia , Bakke Marit Jørgensen , Helgesen Kari Olli , Sundaram Arvind YM , Bakke Sigrid Jørgensen , Kaur Kiranpreet , Horsberg Tor Einar 
Published: April 2020

Abstract
The authors have withdrawn this preprint due to erroneous posting.
Functional hyperemia drives fluid exchange in the paravascular space
Authors: Kedarasetti Ravi , Turner Kevin L. , Echagarruga Christina , Gluckman Bruce J. , Drew Patrick J. , Costanzo Francesco 
Published: April 2020

Abstract
The brain lacks a conventional lymphatic system to remove metabolic waste. It has been proposed that fluid movement through the arterial paravascular space (PVS) promotes metabolite clearance. We performed simulations to understand how arterial pulsations and dilations, and brain deformability affect PVS fluid flow. In simulations with compliant brain tissue, arterial pulsations did not drive appreciable flows in the PVS. However, when the artery dilated as in functional hyperemia, there was a marked movement of fluid. Simulations suggest that functional hyperemia may also serve to increase fluid exchange between the PVS and the subarachnoid space. We measured blood vessels and brain tissue displacement simultaneously in awake, head-fixed mice using two-photon microscopy. Measurements show that brain deforms in response to fluid movement in PVS, as predicted by simulations. Our results show that the deformability of the brain tissue needs to be accounted for when studying fluid flow and metabolite transport.
Antimicrobial resistance and toxigenic profiles of bacteria isolated from tropical shrimps (Farfantepenaeus notialis and Penaeus monodon) in Cameroun
Authors: OMBEDE Sabine Ninelle NGA , KOUDOKPON Hornel , DOUGNON Victorien Tamègnon , DEGUENON Esther , NGOMO Rajeunie Pernelle Jaelle MINDZIE , TCHIBOZO Carine , GNIMATIN Jean Pierre , TCHOUMBOUGNANG François , YADOULETON Anges , DOUGNON Jacques 
Published: April 2020

Abstract
Objective
Post-harvest shrimp losses are a big problem due to the proliferation of spoilage bacteria. Presence and multiplication of these bacteria promotes the emergence of food-borne illnesses. This study was carried out to characterize specifics spoilage bacteria from tropical brackish water shrimp and black tiger shrimp shrimps stored in ambient temperature (25 °C).
Result
22 Bacillus spp; 09 Coagulase negative staphylococci (SCN) and 04 enterobacteria such as 01 Pantoea genus; 01 Serratia plymutica and 02 Serratia rubidaea have been identified. Resistance and virulence genes were searched. All isolates expressed resistance to at least three of antibiotics tested. 03 strains of enterobacteria were succeptible to cetfazidim and Amoxycyclin-Clavulanic Acid. Bacillus showed complete succeptibility to Cefixim, ertapenem and cetfazidim. Staphylocococci were susceptible to clyndamycine. Pantoea spp was resistant to all antibiotics but exhibited intermediate succeptibility to Amoxycyclin-Clavulanic Acid. 04 strains of staphycolocci were positives to mecA resistances genes. None enterobacteria were positives to tetracycline resistance genes. All the strains of Bacillus contained enterotoxin genes, high prevalence of 21 strain to hemolytic enterotoxins and 17 strains to cell-lyse factor production like sphingomyelinase activities. The majority of Bacillus strains present in the study poses a potential risk of food poisoning due to the prevalence of toxin genes found.
Revealing unique inscriptions of a Nazi collaborator in Doodencel 601 of the Oranjehotel
Authors: Wempe Joost , Brink Rick van den , Mooldijk Esmee , Feirabend Nienke , Wiersma Ruben , Sietsma Jilt , Dik Joris 
Published: April 2020

Abstract
During the Second World War the German occupants of the Netherlands made ample use of the Scheveningen prison near The Hague, popularly nicknamed the Oranjehotel . One former death cell in this infamous prison ( Doodencel 601 ) has been preserved in its original condition, showing wartime inscriptions on the cell walls. Interestingly, a small section of the wall has been re-plastered, presumably covering inscriptions. Here, we report on the visualization of this enigmatic text, which so far had escaped the reach of historians. Our visualization methodology was threefold. First, we prepared a cross-section from a drill core to determine the cell-wall stratigraphy. Second, we prepared a physical model wall, mimicking the layering of the original cell wall. Third, we tested a combination of raking-light photography and infrared thermography on the model wall. Applying this methodology on the original wall revealed the inscriptions, including the author’s name Daniël de Blocq van Scheltinga , a prominent Nazi collaborator, as well as a calendar and an important date of his post-war trial in the fall of 1945. Our visualizations flawlessly dovetail with archival findings. Together, they offer an intimate view of an early post-war inmate of the Scheveningen prison, whose message was covered up once the cell was transformed into a war monument in 1946.
Performance of BioFire Array or QuickVue Influenza A+B Test versus a validation qPCR assay for detection of influenza A during a volunteer A/California/2009/H1N1 challenge study
Authors: McIlwain David R. , Chen Han , Apkarian Maria , Affrime Melton , Bock Bonnie , Kim Kenneth , Mukherjee Nilanjan , Nolan Garry P. , McNeal Monica M. 
Published: April 2020

Abstract
Background: Influenza places a significant burden on global health and economics. Individual case management and public health efforts to mitigate the spread of influenza are both strongly impacted by our ability to accurately and efficiently detect influenza viruses in clinical samples. Therefore, it is important to understand the performance characteristics of available assays to detect influenza in a variety of settings. We provide the first report of relative performance between two products marketed to streamline detection of influenza virus in the context of a highly controlled volunteer influenza challenge study. Methods: Nasopharyngeal swab samples were collected during a controlled A/California/2009/H1N1 influenza challenge study and analyzed using for detection of virus shedding using a validated qRT-PCR (qPCR) assay, a sample-to-answer qRT-PCR device (BioMerieux BioFire FilmArray RP), and an immunoassay based rapid test kit (Quidel QuickVue Influenza A+B Test).Results: Relative to qPCR, the sensitivity and specificity of the BioFire assay was 72.1% (63.7%-79.5%, 95% Confidence Interval (CI)) and 93.5% (89.3%-96.4%, 95% CI) respectively. For the QuickVue rapid test the sensitivity was 8.5% (4.8%-13.7%, 95% CI) and specificity was 99.2% (95.6%-100%, 95% CI).Conclusion: Relative to qPCR, the BioFire assay had superior performance compared to rapid test in the context of a controlled influenza challenge study.
A New Method to Better Measure and Interpret Non-Verbal Communication in Patient-Therapist Interactions
Authors: Datz Felicitas , Wong Guoruey , Löffler-Stastka Henriette 
Published: April 2020

Abstract
Objective(s): To develop a novel method of examining facial affects in psychotherapeutic settings and interpreting the subliminal microexpressions and emotions they represent.Results: Therapy sessions are videotaped, and verbal content is evaluated with computer assistance from ATLAS.ti. Visual content is screened for facial action units/micro-expressions using the Facial Action Coding System (FACS) and reevaluated using the Emotional Facial Action Coding System (EmFACS) in order to discern the underlying emotions. Working Alliance Inventory scores are compiled to assess their impact on the quality of the therapeutic relationship. Reliability of facial action unit coding is ensured by a training course and an independently evaluated standardized test at Innsbruck University. Interrater reliability was excellent (Cohen’s κ > 0.80). Application of this method on a small study population to measure non-verbal communication and affects demonstrated its feasibility and usability. We believe this methodology to be widely implementable. Its application in psychotherapy may provide greater insights in investigations into important aspects of therapeutic interaction (e.g. verbal/non-verbal communication between clinicians and patients in psychiatric contexts, quality of psychotherapeutic relationships, etc.), and may be exploitable in clinical psychotherapeutic settings to better understand mechanisms of change in therapy.
Determinants of Micro and Small Scale Enterprises’ financing preference in line with POH and Access to Credit: Empirical Evidence from Entrepreneurs in Ethiopia, Benishangul-Gumuz Regional State
Authors: Meressa Hayelom Abrha 
Published: April 2020

Abstract
Purpose: The purpose of this study was to examine factors that determine micro and small scale enterprises’ financing preference in line with pecking order theory and access to credit in Benishangul-Gumuz Regional State of Ethiopia.Design / Methodology / Approach: The study used primary data collected using cross sectional survey questionnaire followed by mixed research approach. The sample of this study was 296 enterprises selected using proportional stratified random sampling technique. The data was analyzed using descriptive and logistic regression analysis.Findings: The results of logistic regression analysis revealed that business experience, collateral, gender, motivation and enterprises’ sectoral engagement affect financing preference of enterprises in line with pecking order hypothesis. On the second step, only enterprises that need to raise capital through credit were considered to investigate access to credit determinants. Accordingly, the logistic regression result revealed that business experience, size, sectoral engagement, collateral, interest rate, loan repayment period, financial reporting, preparation of business plan, location and educational background of entrepreneurs affect access to credit of enterprises. Research limitations/Implications: More evidence is needed on enterprises’ financing preference and access to credit determinants before any generalization of the results can be made. In addition, the empirical tests were conducted only on 296 entrepreneurs since 2019. Therefore, the results of the study cannot be assumed to extend beyond this group of entrepreneurs to different study periods.Originality/value: This paper adds value to the literature on the determinants of micro and small scale enterprises’ financing preference in line with pecking order hypothesis and access to credit.
Incidence of Idiopathic Cardiomyopathy in Type 2 Diabetes Patients: Age, Sex, and Urbanization status stratified Analysis
Authors: Chen Hua-Fen , Chang Ya-Hui , Lo Hsien-Jung Lo , Li Chung-Yi 
Published: April 2020

Abstract
Background
There are limited data regarding epidemiology of diabetes and idiopathic cardiomyopathy. We investigated the overall and age, sex, and urbanization-specific incidence and relative hazard of idiopathic cardiomyopathy in association with type 2 diabetes in Taiwan.
Methods
A total of 474,268 type 2 diabetes patients were identified from ambulatory care and inpatient claims in 2007–2009 from Taiwan’s National Health Insurance (NHI) database, and 474,266 age-, sex- and diagnosis date-matched controls were randomly selected from the registry of NHI beneficiaries. All study subjects were linked to ambulatory care and inpatient claims (up to the end of 2016) to identify possible diagnosis of idiopathic cardiomyopathy. The person-year approach with Poisson assumption was used to estimate the incidence; and Cox proportional hazard regression model with Fine and Gray’s method was used to estimate the relative hazards of idiopathic cardiomyopathy in relation to type 2 diabetes.
Results
The overall incidence of idiopathic cardiomyopathy for men and women patients, respectively, was 4.26 and 3.34/10,000 person-years, which were higher than the corresponding men and women controls (2.40 and 1.69 per 10,000 person-years). Compared with control group, type 2 diabetes patients were significantly associated with an increased hazard of idiopathic cardiomyopathy (adjusted hazard ratio [aHR] 1.39, 95% confidence interval [CI] 1.28–1.51] in all age and sex stratifications except in those aged > 64 years. Type 2 diabetes aged < 45 years confronted the greatest increase in hazard of idiopathic cardiomyopathy, with an aHR of 2.36 (95% CI 1.68–3.32) and 3.72 (95% CI 1.86–7.44) for men and women, respectively. Patients living in rural areas tended to have higher HRs of idiopathic cardiomyopathy.
Conclusions
In Taiwan, diabetes increased the risk of idiopathic cardiomyopathy in both sexes as well as in all age groups except in those > 64 years. Younger patients and those living in rural areas were especially vulnerable to have higher HRs of idiopathic cardiomyopathy.
Pharmacokinetics comparison of four major bio-active components in normal and blood stasis rats after administration of Naoxintong Capsule by UPLC-TQ/MS
Authors: Li Wei-Xia , Chen Pan-pan , Zhang Shuqi , Li Man-man , Zhang Hui , Wang Xiaoyan , Niu Lu , Tang Jinfa , Li Xuelin 
Published: April 2020

Abstract
Background Blood stasis is one major cause of cardiovascular and cerebrovascular diseases. Naoxintong capsule (NXTC), a Chinese patent medicine, has been widely employed in the prevention and treatment against cardiovascular and cerebrovascular diseases. However, the pharmacokinetics comparison of NXTC in normal and blood stasis rats were remain obscured. Methods Acute blood stasis rats were induced by being placed in ice-cold water during the interval between two injections of adrenaline hydrochloride. Normal and blood stasis rats were administrated of NXTC suspension at the dosage of 5 g⋅kg -1 , and blood was collected at different time points after then. Concentrations of four main components including caffeic acid, ferulic acid, formononetin and tanshinone IIA in rat plasma were quantified by UPLC-TQ/MS. The pharmacokinetic parameters were calculated by Phoenix WinNonlin v6.2 software. Results It was found that C max , AUC all , AUC INF_obs , Vz_F_obs and MRT last of ferulic acid, AUC all, Vz_F_obs and MRT last of caffeic acid in blood stasis rats were significantly different ( P < 0.05) from normal rats. Compared with normal rats, C max of ferulic acid and formononetin decreased significantly in the plasma of acute blood stasis rats, AUC all of caffeic acid and ferulic acid decreased notably, AUC INF_obs of ferulic acid decreased remarkably, Vz_F_obs and MRT last of ferulic acid and caffeic acid increased reversely. It is suggested that the absorption of the four components of NXTC in acute blood stasis rats was weakened, and the elimination time was prolonged. Conclusions The significant difference in some different parameters of the 4 NXTC components in normal and blood stasis rats might be caused by increasing of blood viscosity and slowing down of blood flow in acute blood stasis rats. The pharmacokinetic study under pathological condition provided important information for more rational use of NXTC in clinical situations.
mir-23b: A New Molecular Marker For Neonatal Sepsis In Haemoculture
Authors: Fatmi Ahlam , Rebiahi Sid Ahmed , Chabni Nafissa , Zerrouki Hanane , Azzaoui Hafsa , Elhabiri Yamina , Benmassour Souheila , Ibáñez-Cabellos José Santiago , Aribi Mourad , García-Giménez José Luis , Pallardó Federico V 
Published: April 2020

Abstract
Background: Neonatal sepsis remains an important cause of morbidity and mortality. The ability to quickly and accurately diagnose neonatal sepsis based on clinical assessments and laboratory blood tests remains difficult, where haemoculture is the gold standard for detecting bacterial sepsis in blood culture. It is also very difficult to study because neonatal samples are lacking. Methods : Fifty-four newborns suspected of sepsis admitted to the Neonatology Department of the Mother-Child Specialized Hospital of Tlemcen. From each newborn, a minimum of 1-2 ml of blood was drawn by standard sterile procedures for blood culture. The miRNA-23b level in haemoculture was evaluated by RT-qPCR. Results : miR-23b levels increased in premature and full-term newborns in early onset sepsis ( p < 0.001 and p < 0.005 respectively), but lowered in late onset sepsis in premature and full-term neonates ( p < 0.005) compared to the respective negative controls. miR-23b levels also increased in late sepsis negative versus early sepsis negative controls ( p < 0.05). miR-23b levels significantly lowered in the newborns who died from both sepsis types ( p < 0.0001 and p < 0.05 respectively). Conclusions : The drop in miR-23b levels is an important factor that favours sepsis development, which would confirm their vital protective role, and strongly suggest that they act as a good marker in both molecular diagnosis and patient monitoring.
Incomplete Staging in Ovarian Cancer
Authors: Beltman Jogchum , Laven P , Bense JE , Aa Maaike van der , Vos MC , Boll D , Arts HGJ , Reesink N , Trimbos JB , Kruitwagen RFPM 
Published: April 2020

Abstract
Objective

Incomplete surgical staging of patients with early stage epithelial ovarian cancer (EOC) has been reported in up to 85% of cases, when based on the International Federation of Obstetrics and Gynaecology (FIGO) staging procedure. The aim of the present retrospective study was to clarify the reasons for incomplete staging.

Methods

The PRISMA (Prevention Recovery Information System for Monitoring and Analysis) technique was used to evaluate cases with FIGO I-IIa EOC based on incomplete staging from five gynecologic oncologic center hospitals in the Netherlands in the period 2010-2014.

Results

Fifty cases with an incomplete surgical staging of EOC according to national guidelines were included. The most common reasons for incomplete staging were insufficient random biopsies of the peritoneum (n=34, 68%), and less than ten lymph nodes being resected and/or found at pathology (n=16, 32%). The most mentioned reasons for not performing biopsies were forgetting to do so, and believing that after careful inspection and palpation, taking biopsies is irrelevant and/or already are being taken while performing a hysterectomy (cul-de-sac, bladder). The value of contralateral pelvic lymph node dissection in case of a unilateral ovarian malignancy was also doubted, influencing the number of lymph nodes resected.

Conclusion

The most important reasons for incomplete staging in EOC are, besides omitting elements by accident, questioning the importance of obligatory elements of the staging procedure. A structured list of staging steps during surgery and more evidence based consensus concerning these obligatory elements might increase the number of complete staging procedures in EOC.
Urine neutrophil gelatinase-associated lipocalin and urine output as predictors of the successful discontinuation of continuous renal replacement therapy in critically ill patients with acute kidney injury
Authors: Thomsen Josefine , Sprogøe Ulrik , Toft Palle 
Published: April 2020

Abstract
Background: Continuous renal replacement therapy (CCRT) is a frequently used modality for the support of intensive care patients with acute kidney failure (AKI). Nevertheless, there are no objective criteria for the discontinuation of CRRT. The purpose of this study was to investigate whether urine neutrophil gelatinase-associated lipocalin (uNGAL) alone or in combination with urine output could be used as a diagnostic test for renal function recovery in ICU patients on CRRT.Methods: This was a single-centre prospective observational study including patients with acute kidney failure needing CRRT. Sixty-nine patients were enrolled, and 54 completed the study. Of the 54 patients, 22 recovered renal function (REC), defined as dialysis independency at 72 hours from discontinuation, while 32 patients did not (NREC). Urine NGAL was measured at 0, 6, 12, and 24 hours after CRRT discontinuation. The cumulated urine output was measured for 24 hours prior to discontinuation and at 6, 12, and 24 hours after discontinuation. Logistic regression was used to calculate the missing 6-hour uNGAL values by interpolation. The Youden index was used to calculate cut-off values. A P-value <0.05 was considered statistically significant.Results: Baseline characteristics at CRRT initiation were similar between groups. Compared to the NREC group, REC patients had significantly higher urine output (p<0.0001) and lower uNGAL (p<0.001) at all time points, except for uNGAL at 24 hours (p<0.24). The best single predictor for renal recovery was the cumulated urine output 24 hours after discontinuation (predictive value 83%). Combining uNGAL at 6 hours (cut-off 1650 µg/L) with the cumulated urine output during the 24 hours prior to discontinuation (cut-off 210 ml) proved superior, with predictive values of 92% (CRRT dependency) and 93% (renal function recovery).Conclusions: With predictive values up to 93%, the combination of uNGAL at 6 hours and the cumulatated urine output during the 24 hours prior to CRRT cessation proved to be the best diagnostic test for renal function recovery in ICU patients.Clinical trial registration: N/A