bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses
Authors: Davó Laura , Herrero Laura , Sánchez-Seco Maria Paz , Labiod Nuria , Roiz David , Gómez-Díaz Elena , Hernandez Lourdes , Figuerola Jordi , Vázquez Ana 
Published: May 2020

Abstract
Background
Granada virus belongs to the genus Phlebovirus within the Naples serocomplex and was detected for the first time in sand flies from Spain in 2003. Seroprevalence studies have revealed that Granada virus may infect humans with most cases being asymptomatic. Moreover, recent studies in vector samples revealed that the related Massilia and Arrabida phleboviruses could be also circulating in Spain. The objective of this study was to develop and assess a new sensitive real-time RT-PCR assay for Granada virus diagnosis able to detect the related phleboviruses Massilia and Arrabida.

Methods
Two specific primers and one unique probe to detect Granada, Massilia and Arrabida viruses, without differentiating between them, were designed targeting the conserved L-segment of their genome. Sensitivity was assessed using 10-fold serial dilutions of quantified in vitro DNA samples. Specificity was evaluated by testing different genomic RNA extracted from other representative phleboviruses. The new assay was used for virus detection in sand flies collected in 2012 from the Balearic Archipelago, a touristic hotspot in the Mediterranean.

Results
The real-time RT-PCR assay exhibited a sensitivity per reaction of 19 copies for Granada and Arrabida, and 16 copies for Massilia. No other related phleboviruses were detected. From the 37 pools of sand fly samples studied from four different Balearic Islands, we detected one positive in the island of Cabrera.

Conclusions
To our knowledge, the method described here is the first real-time RT-PCR designed to detect Granada virus and the related Massilia and Arrabida phleboviruses. The study demonstrated that this is a rapid, robust and reliable assay for the accurate diagnosis of human infections as well as for virus surveillance in vectors.
Bacterial community characterization of the rhizobiome of plants belonging to Solanaceae family cultivated in desert soils
Authors: Barraza Aarón , Caamal-Chan María Goretty , Castellanos Thelma , Loera-Muro Abraham 
Published: May 2020

Abstract
Purpose
The plant Solanaceae family is one of the most important for global agriculture and nutrition. Within this plant family, two plant species stand out for their economic importance and for human consumption, which are tomato (Solanum lycopersicum) and chili pepper (Capsicum annuum). Moreover, those plants support diverse and characteristic microbial communities that are uniquely suited to the plant habitat and intimately connected to plant health. The main objective of this work is the bacterial community characterization in the rhizobiome of tomato and chili pepper, cultivated in arid environments.

Methods
Five crop fields located in the south of the peninsula of Baja California, Mexico, were sampled. Total DNA was extracted from rhizosphere, rhizoplane, and endophytic root compartment and sequenced by Illumina MiniSeq platform technology applied to 16S rRNA gene V3 region.

Results
We were able to obtain 1,195,426 total reads and 1,725,258 total reads for tomato and chili pepper samples, respectively. The analysis of the bacterial community structures confirmed that the two plant species showed differences in their microbial community structures. Nonetheless, the microbial community structures were directly and equally influenced by the crop field localization of each plant species. Interestingly, we determined that in both plant species, the Proteobacteria was the main phylum.

Conclusion
In conclusion, we found that several bacterial families are part of the core rhizobiome (28 OTUs) for both tomato and chili pepper, but the most abundant were the Pseudomonadaceae family and the Pseudomonas genus, which most probably play a pivotal role in the microbial ecology to benefit both crop plants.
Camshift tracking method based on correlation probability graph for model pig
Authors: Zhang Xiangnan , Gong Wenwen , He Qifeng , Xiang Haolong , Li Dan , Wang Yawei , Chen Yifei , Liu Yongtao 
Published: May 2020

AbstractThe identification and tracking for model pigs, as a vital research content for studying the habits of model pigs, drawed more and more considerable attention. To fulfill people requirements for the effectiveness of the non-significant model pig tracking in breeding environment, a Camshift tracking approach based on correlation probability graph, i.e., CamTracor−PG, is proposed in this paper, in which the correlation probability graph is introduced to achieve target positioning and tracking. Technically, acquiring images through a vision sensor, according to the circular arrangement of pixels in the inverse probability projection graph, and multiplying the inverse projection probability value of a pixel by its surrounding pixels could obtain the weighted sum. Then, the target projection grayscale graph is established by utilizing the correlation probability value for positioning, identification, and tracking of model pigs. Finally, extensive experiments are conducted to validate reliability and efficiency of our approach.
Sex differences in the association between self-reported sleep duration, insomnia symptoms and cardiometabolic risk factors: cross-sectional findings from Brazilian longitudinal study of adult health
Authors: Silva-Costa Aline , Rotenberg Lucia , Nobre Aline A. , Chor Dora , Aquino Estela M. , Melo Enirtes C. , Barreto Sandhi M. , Schmidt Maria Inês , Griep Rosane H. 
Published: May 2020

Abstract
Background
The U-shaped associations between sleep durations and cardiometabolic risk factors (glycated hemoglobin levels, obesity, hypertriglyceridemia, hypertension and cholesterol levels) are still inconclusive. Moreover, as sleep is comprised of quantitative and qualitative aspects, exploring both insomnia symptoms and sleep duration are relevant when evaluating the potential effects of sleep problems on health. The aim was to evaluate sex-specific associations between sleep problems and cardiometabolic risk factors.

Methods
This cross-sectional study used data from wave two of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil), including 7491 women and 6232 men. Questionnaires were administered to provide information about socioeconomic conditions, lifestyle, and sleep characteristics. A 12-h fasting blood sample was drawn to measure serum cholesterol, triglycerides, and glycated hemoglobin. Blood pressure, weight and height were also measured using standard equipment. Generalized additive models were used to evaluate the curve shape of the relationship between self-reported sleep duration and the outcomes. Logistic regression was performed to investigate the magnitude of the associations of self-reported sleep duration, insomnia symptoms, and short sleep plus insomnia symptoms with cardiometabolic risk factors.

Results
For women, self-reported sleep duration and insomnia symptoms (either separately or linked to short sleep duration) were associated with obesity, hypertension and glycated hemoglobin after adjusting for the confounders. The magnitudes of the associations between self-reported short sleep duration plus insomnia symptoms and the outcomes were slightly increased, considering sleep duration or insomnia symptoms separately. For men, both long sleep duration and insomnia symptoms were associated with hypertriglyceridemia after adjusted for the confounders.

Conclusion
These findings suggest possible sex-specific patterns, since obesity, hypertension and high glycated hemoglobin were associated with self-reported sleep duration and insomnia symptoms in women, but not in men, and reinforce the importance of considering quantitative and qualitative aspects of sleep for the prevention and management of the outcomes.
The spatiotemporal changes of marshland and the driving forces in the Sanjiang Plain, Northeast China from 1980 to 2016
Authors: Li Zhen , Liu Miao , Hu Yuanman , Xue Zhenshan , Sui Jinling 
Published: May 2020

Abstract
Background
Wetland loss is a global concern due to its enormous ecosystem services. Marshland, a typical natural wetland, which is concentrated in the Sanjiang Plain, has undergone dramatic loss in the last several decades. The spatiotemporal changes in marshland were studied based on Landsat images of the Sanjiang Plain from 1980 to 2016 with the land use maps in 1980, 1995, 2000, 2005, 2010, and 2016 using land use dynamic degree and landscape indices. The driving forces of marshland loss, including biophysical factors, socio-economic factors, and land management, were analyzed with boosted regression trees (BRTs) methods.

Results
The area of marshland loss was 7372 km2, which accounted for 65.7% of the area of marshland in 1980; however, the paddy field area was expanded by 22,313 km2. The lost marshland was mainly converted to dry farmland (47.5%) and paddy field (47.2%) during 1980–2016. Both the landscape pattern of the study area and marshland became increasingly fragmented. The relatively important factors responsible for marshland loss were biophysical factors, socio-economic factors, and land management, which accounted for 65.2%, 25.5%, and 8.4%, respectively. The most important driving forces with high “relative influence” were “distance to river,” which accounted for 20.6% of the total variance explained. The “relative influence” of potential crop yield and ditch density reached 20.2% and 8.4%, respectively.

Conclusion
Significant land use changes have occurred in the Sanjiang Plain over the past 37 years, with characteristics of rapid paddy field expansion and drastic marshland loss. Meanwhile, marshland fragmentation continued to increase. Reclamation was the main reason for the large-scale marshland loss. Biophysical factors influenced the decisions regarding the locations of marshland loss. Moreover, climate factors (i.e., average annual precipitation and average annual temperature) also played an important role in marshland loss. These results can provide helpful knowledge for understanding the patterns and reasons for marshland loss and protecting and managing strategies for wetlands restoration.
Prognostic value of lymph node ratio in laryngeal and hypopharyngeal squamous cell carcinoma: A systematic review and meta-analysis
Authors: Abdeyrim Arikin , He Shizhi , Zhang Yang , Mamtali Gulbostan , Asla Aibadla , Yusup Mirkamil , Liu Jiang 
Published: May 2020

Background Several recent studies have indicated that the lymph node ratio (LNR) is an independent prognostic factor for laryngeal and hypopharyngeal squamous cell carcinoma (LHSCC). The purpose of this paper is to assess the prognostic value of LNR and explore appropriate cutoff values by conducting a systematic review and meta-analysis. Methods Pubmed, Embase (via Ovid), and Cochrane library were systematically searched for studies on the prognostic value of LNR in LHSCC up to October 31, 2019. Then, Literature review, data extraction, and quality assessment of eligible studies were performed by two independent reviewers back-to-back. Lastly, Stata 14.0 software was hired to conduct a meta-analysis. Results A total of 445 articles were retrieved, and 13 studies published in English between 2013 and 2019 were included after the title/abstract and full-text screening. Among the 13 studies contributed to 4197 patients, seven studies were about hypopharyngeal squamous cell carcinoma (HPSCC), four studies about laryngeal squamous cell carcinoma (LSCC), and the remaining two studies about LHSCC. The meta-analysis results showed that shorter overall survival (OS) (HR 1.49; 95%CI: 1.18 to 1.88), disease-specific survival (DSS) (HR 1.66; 95%CI: 1.32 to 2.07) and disease-free survival (DFS) (HR 2.04; 95%CI: 1.54 to 2.71) were significantly correlated with a higher LNR in a random-effect model. The cutoff values of eligible studies were varied from 0.03 to 0.14, and the lowest significant LNR was 0.044. Conclusion LNR is a valuable prognostic factor in the survival of LHSCC and may be used to improve the tumor staging systems, which, however, requires the solid support of more high-quality studies.
Unusual presentation of a first Branchial cleft cyst associated with an abnormal bony canal -a case report-
Authors: Fanous A. , Couloigner V. , Gorphe P. , Galmiche L. , Alexandru M. , Garabedian E-N , Coffinet L. , Blanc T. , Leboulanger N. , Denoyelle F. 
Published: May 2020

Background First branchial cleft anomalies are rare, accounting for only 10% of all branchial cleft anomalies. We report an even more rare and unique case of a branchial cleft cyst with features of both first and second arch derivatives. Case presentation A 6-year-old boy presented to us with a left conductive hearing loss associated with pre-tympanic keratin debris and an ipsilateral painful cervical mass. He had a past medical history of left ear surgery for presumed cholesteatoma 2 years prior and left neck abscess drainage 6 months prior. CT and MRI revealed a lesion originating in the external auditory canal and extending cervically through a bony canal located medial to the facial nerve and terminating as a parapharyngeal cyst. The complete removal was accomplished in one surgical stage consisting of three distinct steps: robotic assisted transoral resection of the pharyngeal cyst, an endaural approach and a parotidectomy approach. Conclusion We believe that our detailed description of this rare first branchial cleft cyst with pharyngeal extension, possibly a hybrid case between a first and second branchial cyst, can serve as a valuable tool to Otolaryngologists – Head and Neck Surgeons who come across a similar unusual presentations.
When didactics meet data science: process data analysis in large-scale mathematics assessment in France
Authors: Salles Franck , Dos Santos Reinaldo , Keskpaik Saskia 
Published: May 2020

AbstractDuring this digital era, France, like many other countries, is undergoing a transition from paper-based assessments to digital assessments in education. There is a rising interest in technology-enhanced items which offer innovative ways to assess traditional competencies, as well as addressing problem solving skills, specifically in mathematics. The rich log data captured by these items allows insight into how students approach the problem and their process strategies. Educational data mining is an emerging discipline developing methods suited for exploring the unique and increasingly large-scale data that come from such settings. Data-driven methods can be helpful when trying to make sense of process data. However, studies have shown that didactically meaningful findings are most likely generated when data mining techniques are guided by theoretical principles on subjects’ skills. In this study, theoretical didactical grounding has been essential for developing and describing interactive mathematical tasks as well as defining and identifying strategic behaviors from the log data. Interactive instruments from France’s national large-scale assessment in mathematics have been pilot tested in May 2017. Feature engineering and classical machine learning analysis were then applied to the process data of one specific technology-enhanced item. Supervised learning was implemented to determine the model’s predictive power of students’ achievement and estimate the weight of the variables in the prediction. Unsupervised learning aimed at clustering the samples. The obtained clusters are interpreted by the mean values of the important features. Both the analytical model and the clusters enable us to identify among students two conceptual approaches that can be interpreted in theoretically meaningful ways. If there are limitations to relying on log data analysis in order to determine learning profiles, one of them is the fact that this information remains partial when it comes to describing the complete cognitive activity at play, the potential of technology-enriched problem solving situations in large-scale assessments is nevertheless obvious. The type of findings this study produced is actionable from teachers’ perspective in order to address students’ specific needs.
On the cloud radiative effect for tropical high clouds overlying low clouds
Authors: Kang Hyoji , Choi Yong-Sang , Hwang Jiwon , Kim Hye-Sil 
Published: May 2020

AbstractSince high and low clouds ubiquitously overlie the Tropical Western Pacific (TWP) region, the cloud radiative effect (CRE) cannot be influenced by either high or low clouds, but by combinations of the clouds. This study investigates the CRE of multi-layered clouds in TWP via a radiative transfer model, Streamer. We assumed that multi-layered clouds are composed of full coverage of high clouds overlying low clouds with fractional coverage. The simulation results show that low clouds readily change CREs from positive to negative in the case of optically thin high clouds, even if the fraction of low clouds takes 10% of that of high clouds. Also, various combinations of physical properties of multi-layered high and low clouds allow more CRE variability (− 253.76 to 93.10 W m−2) than single-layered clouds do (− 101.62 to 96.95 W m−2). Even in the same conditions (total column cloud optical thickness = 15 and high cloud top pressure = 200 hPa), the multi-layer clouds have various CREs from − 180.55 to 45.64 W m−2, while the single-layer high clouds − 2.00 W m−2. These findings are also comparable with satellite observations from CERES and CALIPSO. The present study suggests that considerable uncertainty of radiative effects of high clouds over TWP can attribute to low clouds below high clouds.
Biofabrication of silver nanoparticles from Pseudomonas fluorescens to control tobacco mosaic virus
Authors: Ahsan Taswar 
Published: May 2020

AbstractA microbe-based silver nanoparticle could inhibit the tobacco mosaic virus. In the present study, AgNO3 reacts with Pseudomonas fluorescens CZ strain fermentative broth and formed silver nanoparticles. The crystallinity and purity of silver nanoparticles (AgNPs) were examined by an X-ray diffractometer. Number of Bragg reflection was indexed in the face-centered AgNPs to show a cubic structure. Observation of scan electron microscope (SEM) confirmed the formation of AgNPs. Antiviral effects determined by percentage inhibition of local lesion of tobacco mosaic virus (TMV). Spray of AgNPs and inoculation with TMV assayed analysis revealed percentage inhibition of local lesion 92.70. Effects of AgNPs on the morphology of TMV were observed by a transmission electron microscope (TEM). Micrograph images confirmed the significant effects on the morphology of TMV. Biogenic NP from AgNO3 and fermented broth from Pseudomonas fluorescens could be a potent approach to control the TMV.
COVID-19 pandemic: a viewpoint from Asia
Authors: Abdullah-Al-Shafi Md. 
Published: May 2020

AbstractThe expansion of severe acute respiratory syndrome (SARS) coronavirus 2 (SARSCoV-2) has now procured on epidemic percentages, affecting more than 190 nations in a matter of weeks. A widespread SARSCoV-2 contagion begun in Wuhan, Hubei Province, China, and circulate through China and beyond in December 2019. The containment events in China have lessened new instances by more than 90%, but this diminution is not the case to a different place. European countries like Italy and Spain have been the most affected. In Asia, the COVID-19 brings a catastrophe where after China mainland, countries like Iran and South Korea have been affected. There is now severe apprehension concerning the Asian health care system’s ability to effectually counter to the necessities of patients who are infected and need rigorous precaution for COVID-19. The patient’s ratio in special care reported in Iran has perpetually been between 6 and 8% who are rapidly infected. As about South Korea, the ratio is 3% and 4% who are rapidly infected. This analysis emphasizes the epidemiology of COVID-19, its effects in the Asia continent, and active case study of COVID-19 including the distinct countries.
Disparities of demographics, clinical characteristics, and hospital outcomes of AMI pilgrims vs non-pilgrims—tertiary center experience
Authors: Khaled Sheeren , Ahmed Walaa Eldeen , Shalaby Ghada , Alqasimi Hadeel , Ruzaizah Rahaf Abu , Haddad Mryam , Alsabri Mroj , Almalki Seham , Kufiah Heba , Aboul Elnein Fatma , Jaha Najeeb 
Published: May 2020

AbstractBackgroundAcute myocardial infarction (AMI) is usually caused by rupture of an atherosclerotic plaque leading to thrombotic occlusion of a coronary artery. Cardiovascular disease has recently emerged as the leading cause of death during hajj. Our aim is to demonstrate the AMI pilgrim’s related disparities and comparing them to non-pilgrim patients.ResultOut of 3044 of patients presented with AMI from January 2016 to August 2019, 1008 (33%) were pilgrims. They were older in age (P< 0.001) and showed significantly lower rates cardiovascular risk factors (P< 0.001 for DM, smoking, and obesity). Pilgrims were also less likely to receive thrombolytic therapy (P< 0.001), show lower rate of late AMI presentation (P< 0.001), develop more LV dysfunction post AMI (P< 0.001), and have critical CAD anatomy in their coronary angiography (P< 0.001 for MVD and = 0.02 for LM disease) compared to non-pilgrim AMI patients. Despite AMI pilgrims recorded higher rate of primary percutaneous coronary intervention (PPCI) procedures, they still showed poor hospital outcomes (P< 0.001, 0.004, < 0.001, 0.05, and 0.001, respectively for pulmonary edema, cardiogenic shock, mechanical ventilation, cardiac arrest, and in-hospital mortality, respectively). Being a pilgrim and presence of significant left ventricular systolic dysfunction, post AMI was the two independent predictors of mortality among our studied patients (P= 0.005 and 0.001, respectively).ConclusionAlthough AMI pilgrims had less cardiovascular risk factors and they were early revascularized, they showed higher rates of post myocardial infarction complication and poor hospital outcomes. Implementation of pre-hajj screening, awareness and education programs, and primary and secondary preventive measures should be taken in to consideration to improve AMI pilgrim’s outcome.