bluuebunny/crossref_metadata_2025_split huggingface dataset:
An exploratory clinical trial on the safety and efficacy of iNKT cell and PD-1+CD8+ T cell-based innovative immunotherapy in patients with lung adenocarcinoma
Authors: Cheng Xiaobo , Wang Jing , Qiu Chenli , Jin Yanling , Xia Bili , Qin Ran , Hu Huiliang , Yan Jia , Zhang Xiaoyan , Xu Jianqing 
Published: September 2020

Abstract
Background and purposeThe antitumor activities of invariant natural killer T (iNKT) cells and CD8+ T cells have been confirmed. As tumor-specific CD8+ T cells, PD-1+CD8+ T cells kill tumor cells relatively effectively and specifically. We aimed to assess the feasibility, toxicity, antitumor efficacy, and biological correlates of response in patients with advanced lung adenocarcinoma treated with iNKT cells and PD-1+CD8+ T cells for the first time. MethodsWe performed a single-arm, prospective, single-center exploratory clinical trial that is ongoing and registered at ClinicalTrials.gov (NCT03093688). We expanded autologous iNKT cells and PD-1+CD8+ T cells in vitro using our patented technology. Patients were infused with autologous iNKT cells (1×10 8 ~1×10 10 ), PD-1+CD8+ T cells (1×10 7 ~1×10 9 ) and dendritic cells (DCs) (5×10 5 ) every 3-5 weeks, which was considered 1 cycle. The study was conducted from March 1, 2017. The primary endpoint were safety and objective tumor response. ResultsAn analysis of the completed first stage is reported here. A total of 3 consecutive patients were enrolled and received autologous iNKT cell and PD-1+CD8+ T cell-based immunotherapy (≥4 cycles). The first patient received 16 cycles. Computed tomography (CT) examination revealed a stable disease (SD) response after 4 cycles and a progressive disease (PD) response after 11 cycles. The overall survival (OS) time was 20 months. The second patient received 10 cycles. CT examinaton revealed an SD response after 4 cycles and a PD response after 9 cycles. The OS time was 10 months. The third patient received 6 cycles. CT examination revealed an SD response. The OS time was 5.5 months. The patients suffered from only fever, chills, soreness, headache, dizziness, fatigue, sweating, a sick feeling, nausea, vomiting and shortness of breath and received symptomatic treatment. ConclusionsiNKT cell and PD-1+CD8+ T cell-based immunotherapy showed a manageable tolerability proﬁle and effective antitumor activity and was selected as a prospective antitumor therapy.Trial registrationThe protocol had retrospectively registered at clinical trial.gov, Unique identifer: NCT03093688, date of registration: March 01, 2017;https://www.clinicaltrials.gov/ct2/show/NCT03093688?term=NCT03093688&rank=1
Clinical and Prognostic Role of Vasohibin-1 Expression in Solid Tumors
Authors: Tian Ye , Miao Chenkui , Ren Xiaohan , Xu Aiming , Zhu Shenhao , Gu Qi , Hou Chao , Qin Chao , Wang Zengjun 
Published: September 2020

Abstract
BackgroundThe role of Vasohibin-1 (VASH1) expression in solid tumors remains controversial. We thus performed this meta-analysis to elucidate the associations between VASH1 expression and the prognosis of solid tumors.MethodsWe searched relevant literature in PubMed, Web of Science and EMBASE. The hazard ratio (HR) or odds ratio (OR) and 95% confidence intervals (CIs) were measured by fixed-effects or random-effects models. Publication bias was assessed using funnel plots and Egger's regression test.ResultsThe results showed that VASH1 expression exhibited a significantly decreased overall survival (OS) time (HR = 1.85; 95% Cl = 1.27-2.69) and disease-free survival (DFS) (HR = 1.80; 95% Cl = 1.41-2.29) time. Meanwhile, VASH1 expression was found significantly associated with TNM stage (OR = 1.96; 95% CI = 1.57-2.46), tumor stage (OR = 2.35; 95% CI = 1.88-2.93), lymph node metastasis (OR = 2.02; 95% CI = 1.37-2.98), venous invasion (OR = 1.63; 95% CI = 1.00-2.65), tumor grade (OR = 1.74; 95% CI = 1.13-2.67) and microvessel density (MVD) (OR = 4.30; 95% CI = 2.31-8.03). However, no significant association was found between the VASH1 expression and distant metastasis (OR = 1.81; 95% Cl = 0.74-1.41). ConclusionThis study demonstrated that VASH1 expression was relevant to more aggressive clinicopathological parameters and might predict inferior DFS and OS in solid tumor patients.
Identification of Key Candidate Biomarkers and Pathways in Atherosclerosis
Authors: Meng Zhijun , Gao Jia , Liang Hongping , Liu Caihong , Zhao Jianli , Wang Zixuan , Ma Xinliang , Jiao Xiangying , Cao Jimin , Wang Yajing 
Published: September 2020

Abstract
Background Atherosclerosis is the leading cause of cardiovascular disease worldwide for which lacks effective prevention and therapeutic strategy. Therefore, clinical indicators for early diagnosis and screening are in great need. The present study aimed to elucidate the key genetic signatures and pathways identifying the key candidate biomarker in atherosclerosis by integrative bioinformatics analysis combining with experimental assay. Methods The gene expression profiles (GSE30169, GSE6584) were achieved from the Gene Expression Omnibus database. Functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to examine the biological functions of identified differentially expressed genes (DEGs). A protein-protein interaction (PPI) network was mapped using Cytoscape software. Results 91 DEGs were identified, including 68 up-regulated genes and 23 down-regulated genes. Functional enrichment analysis indicated that DEGs genes were significantly enriched in ferroptosis, TNF signaling pathway, IL-17 signaling pathway. 12 nodes with the highest degrees were selected as hub genes. CCL2, CXCL1, IL6, and DUSP1 can serve as a sensitive diagnostic indicator for the early stage of atherosclerosis; CEBPB and HMOX1 can serve as a diagnostic indicator for diabetic atherosclerosis; TRIB3 is a sensitive marker indicating atherosclerosis risks in diabetic women group.Conclusions In conclusion, we have identified key candidate genes that indicate the diagnosis of patients with atherosclerosis, and these genes may serve as potential therapeutic or drug development targets for atherosclerosis.
A Proteomic and Transcriptomic Analysis Reveals That hsa_circ_0004182 Promotes the Expression of Glycerol-3-phosphate Dehydrogenase 1 Like via Targeting mir-155 in Coronary Artery Disease
Authors: Jia Qiaowei , Ji Wenfeng , Zhao Chenhui , Zhou Yaqing , Hou Can , Jia Enzhi 
Published: September 2020

Abstract
Background: Coronary artery disease (CAD) is one of the leading causes of mortality in the world, and its early diagnosis remains a huge challenge in clinical medicine. Genetic and environmental factors both contribute to the occurrence and development of CAD. It is reported that the inhibition of mitophagy plays an important role in CAD, while the molecular mechanism is still uncertain. In our previous studies, circulating mir-155 in plasma showed a significant differential expression in CAD populations and controls. Method: In this study, we detected the expression of RNAs and proteins in human coronary artery samples with genome sequencing. Bioinformatics analysis was conducted to find the binding sites of circRNAs, mir-155 and protein coding genes.Result: 2135 protein groups, 21703 unique peptides and 1640 circRNAs were identified in the samples. 174 differential expressed proteins and 66 differential expressed circRNAs were screened according to the pathological grading and staging of the samples. GO and KEGG analysis showed that the SDEPs were significantly associated with metabolism, oxidative respiration, and mitochondrial function. Based on the gene-gene interaction and gene-protein interaction analysis, a novel ceRNA network may play a regulatory role in CAD. Conclusion: Hsa_circ_0004182 may inhibit the expression of mir-155 by acting as miRNA sponges, and repress the post-transcriptional repression of GPD1L mediated by mir-155, and promote GPD1L expression in coronary artery disease.
Risk of Proximal Femoral Nail Antirotation Implant Failure Upon Different Lateral Femoral Wall Thickness in Intertrochanteric Fracture :A Finite Element Analysis.
Authors: Zheng Liqin , Wong Duo Wai-Chi , Chen Xinming , Chen Yuanzhuang , Li Pengfei 
Published: September 2020

Abstract
PurposePFNA has been commonly used to treat intertrochanteric fractures, despite the risk of implant failure. The integrity of the femur could influence the risk of implant failure. This study aims to evaluate the influence of lateral femoral wall thickness on potential implant failure using a computational modeling approach. MethodsFinite element model of the hip was reconstructed from the Computed Tomography of a female patient. Five intertrochanteric fracture models at different lateral femoral wall thickness (T1 = 27.6 mm, T2 = 25.4 mm, T3 = 23.4 mm, T4 = 21.4 mm, and T5 = 19.3 mm) were created and fixed with PFNA. A critical loading condition was simulated that mimicked a high loading scenario during walking. The implant failure condition, stress and displacement of the PFNA implant and fracture femur were predicted for analysis. ResultsImplant failure of PNFA occurred at the sides of the proximal nail canal especially for the thinner wall models (T4 and T5).The maximum von Mises stress of the nail for T4 changed abruptly to 298.1 MPa. However, thinner wall decreased the displacement of the PFNA implant. There was approximately opponent trend of stress and displacement on proximal and distal fragments with decreasing thickness possibly due to the adaptation after failure.ConclusionA thinner wall increased the risk of PFNA implant failure. Our prediction showed that complete failure occurred when the thickness was 21.4 mm which was close to the value suggested to determine the stability type.
The Role of Dual-Stacked Oxide Semiconductor in High Performance Thin Film Transistors
Authors: Kim Youn Sang , Im Changik , Cho Nam-Kwang , Park Jintaek , Lee Eun Goo , Lee Sung-Eun , Na Hyun-Jae 
Published: September 2020

Abstract
Oxide thin film transistors (TFTs) have attracted much attention because they can be applied to flexible and large-scaled switching devices. Especially, Oxide semiconductors (OSs) have been developed as active layers of TFTs and, among them, Indium-Gallium-Zinc-Oxide (IGZO) is actively used in the OLED display field. However, IGZO TFTs are limited by low field-effect mobility, which critically affects display resolution and power consumption, despite superior off-state properties. Herein, we prevailed new working mechanisms in dual-stacked OS and, based on this, developed dual-stacked OS-based TFT with high field-effect mobility (~80 cm2/V·s), ideal threshold voltage near 0 V, high on-off current ratio (>109), and good stability at bias stress. In dual-stacked OS, induced areas are formed at interface by band-offset: band-offset-induced area (BOIA) and BOIA-induced area (BIA). They connect gate-bias-induced area (GBIA) and electrode-bias-induced area (EBIA), resulting in high current flow. Such mechanism will provide new design rules for high performance OS-based TFTs.
Copeptin combined with National Early Warning Score for predicting survival in elderly patients with acute and critical illness
Authors: Wang Fan , An Wen , Zhang Xinchao 
Published: September 2020

Abstract
Purpose

Copeptin, reflecting vasopressin release, as well as the National Early Warning Score (NEWS), reflecting the severity of critical illness, might qualify for survival prediction in elderly patients with acute and critical illness. This prospective observational study aimed at assessing the predictive value of copeptin combined with NEWS on the prognosis of elderly patients with acute and critical illness.
Methods

We analyzed serum copeptin levels and the NEWS at admission to the emergency department (ED) in a prospective, single-center and observational study comprising 205 elderly patients with acute and critical illness. Death within 30 days after admission to the ED was the primary end point.
Results

The serum copeptin levels and the NEWS in the non-survivor patients group were higher than those in the survivor group [30.35 (14.20, 38.91) vs 17.53 (13.01, 25.20), P = 0.001 and 9.0 (7.0–10.0) vs 7.0 (6.0–8.0), P = 0.001]. Multivariate logistic regression analysis showed that Copeptin, NEWS and copeptin combined with NEWS were all independent risk factors for 30-day mortality in elderly patients with acute and critical illness. Copeptin, NEWS and copeptin combined with NEWS all performed well in predicting 30-day survival, with area under the ROC curve (AUC) values of 0.766 (95%CI, 0.702–0.822), 0.797 (95%CI, 0.744–0.877) and 0.854 (95%CI, 0.798–0.899) respectively. Using the Z test to compare the areas under the above three curves, copeptin combined with NEWS showed a higher predictive value for 30-day survival (P < 0.05). As we calculated, the optimal cut-off value of copeptin and NEWS using the Youden index were 19.78 pg/ml and 8.5 points respectively. Risk stratification analysis showed that patients with both higher copeptin levels and higher NEWS had the highest risk of death.
Conclusions

Copeptin combined with NEWS have a stronger predictive power on the prognosis of elderly patients with acute and critical illness, comparing to either factor individually.
Optical Gating Electron Transport through Nonphotoresponisive Molecular Junctions
Authors: Zhao Zhikai , Guo Chengyang , Sun Feng , Ning Tingyin , Li Zongliang , Xu Bingqian , Zhao Xueyan , Ni Lifa , Wang Qingling , Kang Keehoon , Lee Takhee , Xiang Dong 
Published: September 2020

Abstract
The manipulation of electron transport through single-molecule junctions via light illumination is a critical step towards molecular hybrid devices. However, most kinds of molecules are nonphotoresponsive without photo absorption upon a specific light illumination. Here, a strategy for high efficiently gating electron transport through a nonphotoresponsive molecular junction with a general light source is provided by introducing nanogap plasmons and molecular design. It is found the conductance of the triphenylamine-based molecules, a nonphotoresponsive molecule with buried anchoring groups, can be enhanced by two orders of magnitude under a general light illumination, which should be the greatest enhancement in the family of nonphotoresponsive molecules. It is further revealed that the giant conductance modulation originates from the coupling of the buried anchoring groups and plasmon-excited hot electrons. This work would contribute to the understanding of the interaction mechanisms between light and bridged molecules, assisting the development of the molecule-based hybrid optoelectronic devices.
Why Residents in Southern China Live Longer Than Those in Northern China?
Authors: Wang Mengqi , huangyi yi 
Published: September 2020

Abstract
Background: Life span of human beings is closely related to the natural geographical environment and regional lifestyle. Since 1982, the life expectancy and longevity level in southern China is always higher than northern China, and the standardized mortality rate in northern China is always higher than southern China.
Method: By calculating the contribution of four diseases to longevity in 31 provinces of China, this study found that cardiovascular disease is the main reason for the longevity disparity between northern China and southern China. The population attribution fraction of nine major risk factors of cardiovascular disease in each province were calculated based on their exposure value and relative risk.
Results: residents live in northern China eat more sodium, less vegetable and less sea products, they are more likely to be overweight. PM2.5 in northern China is much higher than in southern China. Cold temperature causes more number of death than hot temperature because of the longer cumulative risk, all these factors lead to higher CVD mortality rate, shorter life expectancy and lower life span in northern China. Diet high in sodium is the leading cause of north-south life span differential.
Conclusions: Changing diet habits, controlling air pollution can effectively reduce the loss of health and life span in northern China.
Examining A Stepped-Care Telehealth Program for Parents of Young Children With Autism: A Proof of Concept Trial
Authors: Wainer Allison L , Arnold Zachary Enos , Leonczyk Caroline , Soorya Latha 
Published: September 2020

Abstract
Background Intervention during the first years of life for children with autism spectrum disorder (ASD) may have the strongest impact on brain development, behavior and long-term functioning. Yet, barriers such as a shortage of trained professionals contribute to significant delays in service. The goal of this proof-of-concept study was to explore strategies that support timely and equitable deployment of ASD-specific interventions. Methods We conducted a 15-week, randomized control trial (RCT) comparing a stepped-care model (i.e. parents first used an interactive website, and then received parent coaching if warranted) of Online Reciprocal Imitation Training (RIT; a naturalistic developmental behavioral intervention) versus a waitlist control condition (WLC). To be eligible, children had to be between 18 to 60 months, meet the cutoff for ASD on the Autism Diagnostic Observation Schedule-2 Edition and demonstrate significant social imitation deficits. Primary outcome measures included child imitation skills (Unstructured Imitation Assessment (UIA)), parental self-efficacy (Early Intervention Parenting Self-Efficacy Scale (EIPSES)), and parent fidelity (RIT Parent Fidelity Form (RIT-PFF)) to the intervention. Secondary outcome measures included child social communication (Social Communication Checklist (SCC)) and family quality of life (Beach Center Family Quality of Life Scale (FQOL)). Results Twenty participants were randomized in a 1:1 fashion, after participant drop out, eight were in the WLC and seven were in Online RIT. Among the primary outcomes, there were significant group differences and improvements in parent fidelity (p= .000), self-efficacy (p=.029), and ratings of child social communication (p=.048) in Online RIT relative to wait-list controls. There was no significant difference of improvement in imitation ability (p=.05) or family quality of life (p=.220)Limitations There are several limitations with this study, including the small sample size, lack of enactment data, and a lack of website engagement data. The WLC design limits the strength of the conclusions that can be drawn.Conclusions Overall, the novel use of stepped-care telehealth shows strong acceptability and holds promise as an innovative delivery model.Trial Registration Trial Registration: ClinicalTrials.gov, NCT04467073. Registered 10 July 2020- Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT04467073
Comprehensive Analysis Using CT&nbsp;Anthropometry Verifies the Association of Psoas and Masseter Muscle With Traumatic Brain Injury
Authors: Cho Hang Joo , Hwang Yunsup , Yang Sei-Yun , Kim Maru 
Published: September 2020

Abstract
Background: Psoas and masseter muscle are known markers for sarcopenia. However, superiority between them is controversial. Authors planned study to analyze them with prognosis of patients with traumatic brain injury. (TBI)Methods: Patients with TBI visiting regional trauma center between 2017 January to 2018 December were selected and their medical records were reviewed. TBI patients with abbreviated injury score (AIS) 4 or 5 were selected. Patients with AIS 4 or 5 at chest, abdomen and extremity were excluded. And Patient with hospital stay 1 or 2 days were excluded. Both muscle area were measured at their initial computed tomograpy and psoas muscle index (PMI) and masseter muscle index (MMI) were calculated by both muscle area divided by height squared. (cm2/m2) These muscular parameters along with other medical information were analyzed with mortality and Glasgow outcome scale. (GOS)Results: After exclusion process, total 179 patients were included to statistical analyses. Their mean age was 58.0 years, and there are 147 male patients. (82.1%) There were 30 patients with mortality. (16.8%) Mean GOS were 3.7 retrospectively. Parameters were analyzed with mortality which is defined as a qualitative outcome. PMA (16.9 vs. 14.4 cm2, P = 0.028) and PMI (5.9 vs. 5.1 cm2/m2, P = 0.004) showed statistical difference. PMI also showed its statistical significance as a risk factor of mortality in logistic regression analysis. (B = -0.336, P = 0.023, Odds ratio (95% confidence interval) = 0.715 (0.535 – 0.954) Also analyses was performed with GOS which is defined as a quantitative outcome. Bivariate correlation analysis showed statistical correlation between PMI and GOS. (correlation coefficient = 0.168, P = 0.003) And PMI (B = 0.184, P = 0.004, variation inflation factor 1.001) showed its significance in multiple regression analysis additionally.Conclusions: Bigger PM was associated survival and better GOS statistically and could show its superiority as a prognostic marker. Further analyses with more number of patients, more parameters and more precise setting could yield better outcome and increase understanding of relation between sarcopenia and TBI.
Carriage of Cdt-B Encoding Campylobacter spp., Salmonella Enterica, and Yersinia Entercolitica in Patients With Gastroenteritis and Irritable Bowel Syndrome
Authors: Ganji Leila , Shirazi Mohammad Hassan , Alebouyeh Masoud , Eslami Parisa , Rahbar Mohammad , Daryani Nasser Ebrahimi , Zali Mohammad Reza 
Published: September 2020

Abstract
Introduction: Cytolethal distending toxin (Cdt) is one of the bacterial toxins that present in a variety of Gram-negative human pathogens, such as E. coli, Salmonella spp., and Campylobacter spp. CDT composed of three subunits encoded by three adjacent genes, including cdtA, cdtB and cdtC. It is approved that cdtB had toxic activity and caused DNA damage of the host cell. Despite its presence in different bacterial species, role of Cdt in acute and chronic infections, such as gastroenteritis and irritable bowel syndrome (IBS) is unclear. To analyze this correlation, we studied the prevalence of cdtB among different enteropathogenic bacteria in patients with gastroenteritis and IBS compared with healthy people.Materials and Methods: In this cross-sectional descriptive study, 230 stool samples were collected from patients with gastroenteritis, IBS, and healthy people. The presence of Cdt-B encoding bacteria, including Escherichia coli, Campylobacter spp., Yersinia entercolitica, Providencia alkalifacience, and Salmonella enterica was examined by polymerase chain reaction using genus specific primers. Results: Out of 230 stool samples, Cdt-B encoding Campylobacter spp. were found in 34.6% (52/150), 6.25% (5/80), and 4% (2/50) of the patients with gastroenteritis, IBS, and the control group, respectively. Carriage of Cdt-B encoding Salmonella enterica was characterized among 5.3% (8/150) of the patients with gastroenteritis and 17.5% (14/80) of the IBS patients. Although none of the patients carried cdtB of E. coli and Providencia spp., cdtB of Y. enterocolitica was detected in 1 of the patients with gastroenteritis (0.6%). Statistical analysis showed significant correlation between infection with CdtB-encoding Campylobacter spp. and IBS-D subtype. No significant correlation was found between infection with Cdt-B encoding bacteria, and other clinical and demographic data.Conclusion: Our results confirmed relatively higher frequency of Cdt-B encoding bacteria in the intestine of IBS patients and those with gastroenteritis compared with healthy individuals. Regarding the frequency of Cdt-B encoding Salmonella and Campylobacter bacteria, it was proposed that infection with these enteropathogens could be considered as a risk factor for the development or progression of IBS among the Iranian patients. Further studies are needed to establish this involvemet.