bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interleukin (IL)-1 family cytokine in primary immune thrombocytopenia: a comparison with systemic lupus erythematosus-associated thrombocytopenia
Authors: Zhan Yanxia , Wu Boting , liu Chanjuan , Cheng Luya , Ji Lili , Li Feng , Ke Yang , Chen Pu , Hua Fanli , Yuan Ling , Min Zhihui , Sun Lihua , Chen Hao , Cheng Yunfeng 
Published: March 2020

Abstract
Background : Primary immune thrombocytopenia (ITP) is an autoimmune-mediated disorder characterized by decreased platelet count. Systemic lupus erythematosus (SLE) is also an autoimmune disease which thrombocytopenia is a common hematologic manifestation. Interleukin (IL)-1 family cytokines are major proinflammatory and immunoregulatory mediators. This study aimed to investigate the role of IL-1 cytokines in patients with ITP and SLE and the potential pathophysiologic mechanism to differentiate SLE-associated thrombocytopenia (SLE-TP) from ITP. Methods : Multiplex cytokine assay and real-time polymerase chain reaction (RT-PCR) were used to measure the IL-1 cytokines in 17 newly diagnosed ITP patients, 17 SLE-TP patients, 19 SLE patients without thrombocytopenia (SLE-NTP) and 10 healthy controls. Results : The serum levels of IL-1β, IL-18, IL-36α, IL-36β, IL-36γ and IL-33 were decreased significantly in ITP patients as compared with SLE-TP, SLE-NTP patients and healthy controls ( p <0.05). There was no significantly difference in the serum level of IL-37 between ITP and SLE-TP patients, however, there is a positive correlation between platelet count with IL-37 level in ITP patients. Our data suggested that serum IL-1β, IL-18, IL-36α, IL-36β, IL-36γ, IL-33 and IL-37 were involved in the pathogenesis of ITP. Conclusions : Serum IL-1β, IL-18, IL-36α, IL-36β, IL-36γ and IL-33 could be considered biomarkers to differentiate SLE-TP from ITP patients.
TREM-1 modulates dendritic cell maturation and dendritic cell–mediated T cell activation induced by ox-LDL
Authors: Wang Yunkai , Wang Jie , Han Lu , Shen Yun Li , You Jie Yun , Zhang Chun Yu , Liu Xian Dong , Zhang Qi 
Published: March 2020

Abstract
Background: Triggering receptor expressed on myeloid cells (TREM)-1is identified as a major upstream proatherogenic receptor. However, the cellular processes modulated by TREM-1 in the development of atherosclerosis and plaque destabilization has not been fully elucidated. In this study, we investigated the effects of TREM-1 on dendritic cell maturation and dendritic cell–mediated T-cell activation induced by oxidized low-density lipoprotein (ox-LDL) in atherogenesis. Methods: Human peripheral blood monocytes were differentiated to dendritic cells and stimulated by ox-LDL. Naive autologous T cells were co-cultured with pretreated dendritic cells.The expressionof TREM-1 and the production of inflammatory cytokines were assessed by real-time PCR, western blot and ELISA.The expression of immune factors was determined with FACS to evaluate dendritic cell maturation and T-cell activation. Results: Stimulation with ox-LDL promoted dendritic cell maturation, TREM-1 expression and T-cell activation, and exposure of T cells to ox-LDL-treated dendritic cells induced production of interferon-γ and IL-17. Blocking TREM-1 suppressed dendritic cell maturation with low expression of CD1a, CD40, CD86 and HLA-DR, decreased production of TNF-α, IL-1β, IL-6 and MCP-1, and increased secretion of TGF-β and IL-10. In addition, stimulation of ox-LDL induced miR-155, miR-27, Let-7c and miR-185 expression, whereas inhibition of TREM-1 repressed miRNA-155. Silencing TREM-1 or miRNA-155 increased SOCS1 expression induced by ox-LDL. T cells derived from carotid atherosclerotic plaques or healthy individuals showed similar result patterns. Conclusion: These data suggest that TREM-1 modulates maturation of dendritic cells and activation of plaque T cells induced by ox-LDL, a pivotal player in atherogenesis.
Uni-port total thoracoscopic surgery versus median sternotomy for redo tricuspid valve replacement: A retrospective study
Authors: Yan Licheng , Zheng Fuzhen , Chen Haiyu , Bao Jiayin , Weng Guoxing 
Published: March 2020

Abstract
BACKGROUNDː This study compared the perioperative and follow-up period data of patients who underwent redo tricuspid valve replacements performed via thoracoscopic surgery or median sternotomy. The purpose was to evaluate the feasibility, safety, and surgical outcomes of redo tricuspid valve replacement via uni-port thoracoscopic surgery. METHODSː Forty-nine patients with severe tricuspid valve regurgitation after left-side valve replacement underwent redo tricuspid valve replacements in our hospital from April 2012 to September 2019. 26 patients underwent uni-port total thoracoscopy surgery, whereas 23 patients had the surgery performed via median sternotomy. We collected perioperative and 3- to 36-month postoperative data. RESULTSː No deaths occurred in the intraoperative period. Time of cardiopulmonary bypass in the study group was significantly longer than that in the control group (P<0.05), but the operative times in the study and control groups were not significantly different. Thoracic drainage, length of ICU stay, postoperative hospital stay and complication rates in the study group were significantly different from those in the control group (P<0.05). Throughout the follow-up period, uni-port total thoracoscopic TVR is not inferior to traditional surgery with respect to cardiac function and recurrence of tricuspid valve regurgitation. CONCLUSIONSː Uni-port total thoracoscopic tricuspid valve replacement is safe, feasible and effective, and that can be considered as a primary treatment strategy for patients with severe TR after previous left-sided valve procedure.
Long non-coding RNA LINC00662 facilitates bladder cancer progression through modulating miR-195-5p/VEGFA/Ras/Raf/MEK/ERK signaling pathway
Authors: Wang Hengbing , Niu Xiaobing , Jiang Hesong , Zhong Bing , Jiang Xi , Fu Guangbo 
Published: March 2020

Abstract
Background: Bladder cancer (BC) is one of the most common malignant tumors in the urinary system. Long non-coding RNA (lncRNA) plays an important role in BC. Methods: LINC00662 expression was identified by quantitative real-time polymerase chain reaction (qPCR) and in situ hybridization (ISH). The effect of LINC00662 on cell proliferation, apoptosis, migration and invasion was measured by CCK8 assay, colony formation assay, transwell assay, and western blot. Dual-luciferase reporter gene assay, RNA pull-down and RIP assay confirm the interaction between LINC00662 and miR-195-5p/VEGFA. The in vivo effect of LINC00662 on BC was investigated using a mouse tumorigenicity model. Results: LINC00662 was overexpressed in BC tissues and cell lines, and negatively correlated with the survival of BC patients. Overexpression of LINC00662 promoted proliferation, migration and invasion and inhibited apoptosis of BC cells, and LINC00662 knockdown abrogated this effect. Silencing LINC00662 inhibited BC growth in a subcutaneous BC tumor mouse model. LINC00662 acted as an oncogene through regulating miR-195-5p/VEGFA axes. Decreased VEGFA expression caused by LINC00662 knockdown inhibited the phosphorylation of Raf-1, MEK1/2, and ERK1/2, while this regulation effect was abrogated by miR-195-5p inhibitor. Conclusion: LINC00662 regulated the proliferation, apoptosis, migration, and invasion of BC cells probably via miR-195-5p-mediated VEGFA/Ras/Raf/MEK/ERK signaling pathway.
Synthesis and Structural characterization of hydroxyapatite nanorods: Antibacterial activity and dermal irritation
Authors: Domínguez G. García , Hernández Aristeo Garrido , Torre S. Díaz de la , Guitrón L. Chávez , Quezada M. Cruz , Hernández E. Vergara 
Published: March 2020

Abstract
Hydroxyapatite (HA) nanoparticles with homogeneous rod morphology were successfully synthesized by the hydrothermal method. The use of cationic and anionic surfactants (cetyltrimethylammonium bromide, CTAB, and sodium dodecyl sulfate, SDS) does not change the morphology, but the length/diameter ratio of HA nanorods did. The structural characterizations revealed well-crystallized samples in the hexagonal phase. The HA samples with a smaller Ca/P ratio presented larger crystalline size. The CTAB- sample present antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Streptococcus anginosus, Staphylococcus aureus, Micrococcus luteus, and Klebsiella pneumoniae suggesting that the bacteria nature and surface charge of nanoparticles affect the susceptibility.
Estimating hemodynamic risks of tandem stenoses in posterior cerebral circulation: a computational study
Authors: Liu Haipeng , Wang Defeng , Leng Xinyi , Lan Linfang , Ip Vicent Hing Lung , Wong Lawrence Ka Sing , Zheng Dingchang , Chen Fei , Shi Lin , Leung Thomas Wai Hong 
Published: March 2020

Abstract
Background: Tandem stenoses were widely observed in acute stroke cases. High pressure drop and low fractional flow (FF) indicate the risk of ischemia caused by intracranial atherosclerotic stenosis. Low wall shear stress (WSS) and high low-density lipoprotein (LDL) filtration rate indicate the risk of atherosclerotic plaque growth. High WSS is related to the risk of plaque rupture. The hemodynamic parameters (pressure drop, FF, WSS, and LDL filtration rate) could be estimated using computational fluid dynamics (CFD) simulation. Few studies have investigated the hemodynamics of intracranial tandem stenoses. This study aims to estimate the clinical risks of intracranial tandem stenoses using CFD simulation.Methods: With identical radius and length, 4 cylindrical tube models were developed with different stenoses (severity in area: 75% single, 75%-84% tandem, 75%-84%-89% tandem, and 89% single). The pressure drop and FF of each stenosis were derived from static CFD simulation and compared between models. A transient CFD simulation was performed on a patient-specific intracranial arterial model with three (56.2%, 51.3%, and 89.8%) tandem stenoses using boundary conditions derived from patient-specific measurements. For the three stenoses, pressure drop, FF, WSS, and LDL filtration rate were calculated and compared.Results: Independent from other stenoses, 89% single stenosis caused comparable pressure drops in four tube models (difference less than 10%), larger than those of 75%-84% tandem stenoses. 89% stenosis had the lowest FF (less than 0.85). In the patient-specific model, the 56.2% and 51.3% stenoses had pressure drops less than 2mmHg with FFs higher than 0.98, while the 89.8% stenosis caused a pressure drop more than 10mmHg with FF lower than 0.90, where a high WSS belt extended distally. Compared with low WSS (less than 0.5Pa), high LDL filtration rate appeared in smaller areas on arterial wall, with less temporal fluctuations.Conclusions: 1. Tandem stenoses are independent in pressure drop and FF. Pressure drop increases sharply with the severity of stenosis. 2. Compared with low WSS, high LDL filtration rate is more specific and stable in reflecting the risk of intracranial atherosclerotic plaque growth. 3. The risks of both plaque growth and rupture should be considered in severe stenoses.
Investigation of Human Cardiac Fiber Architecture with Multiple diffusion MRI schemes at Multi-Scales based on Simulation Data
Authors: DU Hongbo , wang Lihui , HUANG Jianping 
Published: March 2020

Abstract
Background Diffusion tensor imaging (DTI), diffusion spectrum imaging (DSI) and Q-ball imaging (QBI) are currently three main diffusion MRI (dMRI) schemes available for non-invasive investigation of cardiac fiber architecture. Although DSI and QBI have undoubtedly greater potential to reveal complex cardiac fiber structures than DTI, it however remains unclear to which level and at which scale they provide more gain for investigating cardiac fiber structure.Method This work attends to provide a quantitative description of cardiac fiber architecture derived from different schemes at various scales. Due to the limit of the spatial resolution of clinical MRI scanner and with the absence of the ground-truth, it is difficult to give the accurate description. To deal with this issue, we simulate firstly DTI, DSI and QBI of a cardiac fiber model with the structure a priori known at different scales, and then the estimation accuracy, the diffusion metrics and the helix and transverse angles of cardiac fiber obtained by different schemes at different scales are calculated. Results The results show that although DSI and QBI can distinguish multiple fiber orientations, they are readily to generate false positive and false negative fibers which influence therefore the estimation accuracy. When there are multiple fiber orientations in one voxel, the diffusion anisotropy detected by DTI is higher than DSI and QBI, the range of helix and transverse angle decreases with increasing of the scales, and that detected by DSI is larger than DTI and QBI. Conclusion The results showed that the proposed dMRI simulator provides a valuable tool for simulating realistic DW images of whole human hearts, which can be used as the gold standard to study the fiber structures of the heart.
Effects of Wearing Raised Heel Insoles (RHI) for a Long Term on Physical Functions: Focused on an Adult Male in Their Twenties
Authors: Cha Yu-Jin 
Published: March 2020

Abstract
Background: The objective of this study was to evaluate the effects of the long-term wear of raised heel insoles (RHI) on the changes in overall body functions and mechanical adaption.Methods: This study measured pelvic range of motion (ROM), balance, sensory, ankle joint ROM, lower back pain (LBP), muscle tone, and the muscle stiffness and decrement of male subjects in their 20s. The subjects wore RHI (5cm) for 8 hours per day for 8 weeks (60 days) and measurements were conducted three times (0th, 30th, and 60th days from the experiment start date).Results: Pelvic ROM increased and static balance decreased when the duration of wearing the RHI increased. Moreover, when the duration of wearing the RHI increased, the sensory function of two-point discrimination (2PD) decreased, the state of tension of the medial gastrocnemius (MG) increased, and the dynamic stiffness worsened. Conclusions: This study provides baseline data for presenting guidelines for preventing the musculoskeletal deformity and degeneration of the body by revealing changes in overall physical functions due to the extended wearing of RHI.
Non-steroidal anti-inflammatory drug-induced enteropathy as a major risk factor for small bowel bleeding
Authors: Lim Doo-Ho , Jung Kyoungwon , Lee Seung Bum , Park In Kyu , Cha Hee Jeong , Park Jae Ho , Kim Byung Gyu , Jung Seok Won , Du Jeong In , Kim Jae Hyun , Kim Sung Eun , Moon Won , Park Moo In , Park Seun Ja 
Published: March 2020

Abstract
Background: Small bowel (SB) bleeding accounts for 5% of all gastrointestinal (GI) bleeding cases and 80% of obscure GI bleeding cases. Although angioectasia is the common etiology of SB bleeding, nonsteroidal anti-inflammatory drug (NSAID)-induced SB lesions are also reported as a major cause in studies from Eastern countries. Herein, we assessed the frequency of occurrence of NSAID-induced SB lesions in Korean patients with obscure GI bleeding.Methods: We retrospectively analyzed medical records of all consecutive patients aged ≥18 years that underwent capsule endoscopy from March 2018 to February 2019 at Ulsan University Hospital and Kosin University Gospel Hospital.Results: Of the 83 subjects (all were Korean; mean age ± standard deviation: 59 ± 18 years; age range: 18–84 years; men: n=52; women: n=31), 55 (66.2%) had stools with clear blood and 28 (33.8%) had normal stools with iron deficiency anemia . The detection rate of SB bleeding and lesions using capsule endoscopy was 72.3% (60 of 83 patients). A significantly higher frequency (40 of 51) of ulcerative/erosive lesions than other causes was observed in patients with inactive bleeding but visible SB lesions. As a result, NSAID-induced enteropathy accounted for 41.7% (25 of 60) of all SB bleeding cases.Conclusions: Contrary to what is reported for patients in Western countries, this study in Korean patients showed an improved diagnostic yield of capsule endoscopy for obscure GI bleeding and that NSAID-induced enteropathy was the most common etiology of SB bleeding. Aggressive small intestine examination is required for patients with unexplained GI bleeding.
The influence of time of elimination communication on the prevalence of infantile daytime urination control - a survey from Mainland China
Authors: Wang JianJian , Wang Yan , Wang Yi He , Wen Yi Bo , Jiang Chun Zi , Wen Jian Guo 
Published: March 2020

Abstract
Background: Child self-administration of urination is one of the most challenging stages of development in early childhood, and it is also an important manifestation of family development in parenting. But a pilot survey shows that urination control has decreased significantly during the past decade in Mainland China.Whether it is related to delay of elimination communication (EC) is unclear.Methods A cross-sectional study in children (aged 3-10 years) was performed by distributing 10 010 anonymous self-administered questionnaires to parents in Mainland China from March to September 2017. T The questionnaires included sociodemographic data,caregivers’ information,and details about the disposable diapers (DD) usage,EC commencement date.Results A total of 8 629 ( 86.22% ) children were qualified to enter the final statistical analysis.The urinary control rates at 2 years old in children with EC starting before 12 months of age was significantly higher than those who start after 12 months of age ( 70.72 %vs 59.02 %,p<0.001),and significantly higher than those no EC ( 70.72 %vs 42.48 %,p<0.001). In addition,there was no significant difference in the rate of urinary control at 2 years old between the subgroups at different EC start times within 12 months. After 12 months, the urinary control rate at 2 years old of different subgroups decreased with the start time of EC, which were: 59.97% and 54.10%. The results of urinary control rates at several other ages (ie, 0.5 years,1 year,and 1.5 years) are similar to those at 2 years of age. There was no difference between groups of different genders. The later the beginning of EC, the lower the urination control was found . EC helps infants move quite easily into traditional toilet training when they are old enough.Conclusions Infants and young children start EC as soon as possible at the age of 3 to 12 months is conducive to the development of daytime urination control. A later onset of EC may be risk factors for urination control.
Is Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy Indicated in Hepatobiliary Malignancies?
Authors: Leigh Natasha , Solomon Daniel , Pletcher Eric , Labow Daniel M. , Magge Deepa R. , Sarpel Umut , Golas Benjamin J. 
Published: March 2020

Abstract
Background Hepatopancreaticobiliary malignancies with peritoneal carcinomatosis (PC) exhibit poor survival with current therapies; HCC 11 months with sorafenib, pancreaticobiliary 9-14 months with systemic chemotherapy. Methods We retrospectively reviewed our institutional hepatopancreaticobiliary malignancies with PC which underwent CRS/HIPEC from 2007-2017 and analyzed perioperative and oncologic outcomes. Results 17 patients were included; 9 HCC, 8 pancreaticobiliary (4 cholangiocarcinoma, 3 gallbladder, 1 pancreatic). PCI (12 vs. 9), completeness of cytoreduction (78% vs. 75%) and 30-day morbidity (11% vs. 13%) were equivalent. HCC received less neoadjuvant therapy (11% vs. 75%, p=0.008), though adjuvant therapy rates were similar (78% vs. 100%, p=0.156). At a median follow-up of 15 months, PFS was similar (7 vs. 10 months p=0.957). However, OS was longer in HCC (42 vs. 15 months p=0.206) with 59% 3-year OS (vs. 0% pancreaticobiliary). Conclusions CRS/HIPEC may offer a survival benefit in select HCC patients with PC, though has dubious utility in PB malignancies.
Coexistence of lung squamous cell carcinoma and pulmonary tuberculosis within a single lesion: a case report
Authors: Xiao Guoliang , Chen Shengjie 
Published: March 2020

Abstract
Background: Tuberculosis and lung cancer are common high-mortality lung diseases, but it is rare that lung cancer and tuberculosis exist in the same lesion. In addition, lung cancer with pulmonary tuberculosis is similar in morphology to lung cancer without tuberculosis. For these reasons, lung cancer patients with tuberculosis are often misdiagnosed and mistreated, so these patients have a poorer prognosis than lung cancer patients without tuberculosis. Therefore, a full understanding of such cases can help early diagnosis, so that appropriate treatments can be performed timely. Case presentations: A 52-year-old male. The physical examination revealed a cavity-like nodule in the upper left lung and the first diagnosis was tuberculosis. There was no significant change in lung nodules after anti-tuberculosis treatment. So the patient was admitted to the hospital for surgical treatment of lung cancer. Postoperative pathological showed that the lesion was lung squamous cell carcinoma with tuberculosis.Conclusion: the survival rate of patients with active pulmonary tuberculosis and lung cancer is lower than those with lung cancer or tuberculosis alone. Which is mainly due to incomplete diagnosis. if we find hollow shadows in the lungs on CT, we should think about the possibility of coexistence of lung cancer and tuberculosis, and then the appropriate treatment and management strategies should be developed.
INTRABEAM intraoperative radiotherapy combined with portal vein infusion chemotherapy for treating hepatocellular carcinoma with portal vein tumor thrombus
Authors: Song Xiaodong , He Yong , Liang Huihong , Han Menling , Shao Zili 
Published: March 2020

Abstract
Background: Portal vein tumor thrombus (PVTT) is common in hepatocellular carcinoma (HCC). Recent studies indicate that more aggressive treatments, including surgical resection or locoregional treatment, may benefit select HCC patients with PVTT. External radiation therapy and infusion chemotherapy were found to achieve good outcomes; however, the use of low-energy x-ray radiation system (INTRABEAM), intraoperative radiation therapy, and portal vein infusion chemotherapy for PVTT has not been reported. Case Summary: We present a case of HCC with PVTT. The patient underwent hemihepatectomy and thrombectomy along with intraoperative radiotherapy (IORT) using a portable INTRABEAM radiation system. Subsequently, to treat PVTT, portal vein infusion chemotherapy with of FOLFOX (leucovorin [Folinic acid], fluorouracil, and oxaliplatin) regimen was administered. There were no obvious post-operative complications. After 14 months follow-up period, no obvious tumor recurrence had been observed, and PVTT gradually disappeared completely. Conclusions: IORT using the INTRABEAM radiation system combined with portal vein infusion chemotherapy is promising for select patients with PVTT.