bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analog of multiple electromagnetically induced transparency using double-layered metasurfaces
Authors: Liu Siyuan , Xu Zhixia , Yin Xiaoxing , Zhao Hongxin 
Published: May 2020

AbstractWe reported an analog of electromagnetically induced transparency (A-EIT) featured by double transparent peaks in the spectrum. The A-EIT is realized by double-layered metasurface which consists of spoof localized surface plasmons (S-LSP) and cut-wire (CW)-square rings (SR) hybrid. Electric and magnetic S-LSP are excited as bright and dark modes respectively then couple with resonant modes of CW and SR simultaneously to achieve multiple A-EIT. Two bright modes of the electric S-LSP and SR are excited by external electric field directly that produce a bright-bright mode A-EIT. Moreover, the magnetic S-LSP, which cannot be excited by external field directly, is excited through near field coupling from CW, inducing another bright-dark mode A-EIT. Theoretical analysis with corresponding experiment in microwave band are introduced for better insights into physical essence of the double-peaks A-EIT.
Combination of Serological, Antigen Detection, and DNA Data for Plasmodium falciparum Provides Robust Geospatial Estimates for Malaria Transmission in Haiti
Authors: Oviedo Adan , Knipes Alaine , Worrell Caitlin , Fox LeAnne M. , Desir Luccene , Fayette Carl , Javel Alain , Monestime Franck , Mace Kimberly , Chang Michelle A. , Udhayakumar Venkatachalam , Lemoine Jean F. , Won Kimberly , Lammie Patrick J. , Rogier Eric 
Published: May 2020

AbstractMicroscopy is the gold standard for malaria epidemiology, but laboratory and point-of-care (POC) tests detecting parasite antigen, DNA, and human antibodies against malaria have expanded this capacity. The island nation of Haiti is endemic for Plasmodium falciparum (Pf) malaria, though at a low national prevalence and heterogenous geospatial distribution. In 2015 and 2016, serosurveys were performed of children (ages 6–7 years) sampled in schools in Saut d’Eau commune (n = 1,230) and Grand Anse department (n = 1,664) of Haiti. Children received malaria antigen rapid diagnostic test and provided a filter paper blood sample for further laboratory analysis of the Pf histidine-rich protein 2 (HRP2) antigen, Pf DNA, and anti-Pf IgG antibodies. Prevalence of Pf infection ranged from 0.0–16.7% in 53 Saut d’Eau schools, and 0.0–23.8% in 56 Grand Anse schools. Anti-Pf antibody carriage exceeded 80% of students in some schools from both study sites. Geospatial prediction ellipses were created to indicate clustering of positive tests within the survey areas and overlay of all prediction ellipses for the different types of data revealed regions with high likelihood of active and ongoing Pf malaria transmission. The geospatial utilization of different types of Pf data can provide high confidence for spatial epidemiology of the parasite.
Soybean yield, nutrient uptake and stoichiometry under different climate regions of northeast China
Authors: Zhao Shicheng , Xu Xinpeng , Wei Dan , Lin Xiaomao , Qiu Shaojun , Ciampitti Ignacio , He Ping 
Published: May 2020

AbstractClimate and soil fertility influence seed yield, nutrient uptake, and nutrient stoichiometry in the plant. We collected soybean [Glycine max (L.) Merr.] data were collected from field experiments in northeast China (warm and cold regions) to study the effect of temperature variations during the crop growing season on seed yield, nutrient uptake and stoichiometry from 2001 to 2017. Soybean seed yield has been increased in the cold region but not in the warm region, where average seed yield was higher. The indigenous nitrogen (N) supply followed the same trend as yield, greater in warmer environments but also increasing over time. The internal efficiency (IE) of N and potassium (K) performed similarly in both climate regions, but phosphorus (P) IE was 30% greater in the warm region than the cold region. For soybean nutrient uptake ratio, the N/K ratio was similar between both regions; however, the N/P ratio was greater in the warmer region relative to the colder region. Overall, the higher temperature experienced in the warm region increased soybean seed yield relative to the cold region, and high soil P accumulation caused soybean P luxury uptake in the cold region of northeast China.
Thermodynamic interference with bile acid demicelleization reduces systemic entry and injury during cholestasis
Authors: de Oliveira Cristiane , Khatua Biswajit , El-Kurdi Bara , Patel Krutika , Mishra Vivek , Navina Sarah , Grim Bradley J. , Gupta Srishti , Belohlavek Marek , Cherry Brian , Yarger Jeffery , Green Matthew D. , Singh Vijay P. 
Published: May 2020

AbstractBile acids (BA), with their large hydrophobic steroid nucleus and polar groups are amphipathic molecules. In bile, these exist as micelles above their critical micellar concentration (CMC). In blood at low concentrations, these exist as monomers, initiating cellular signals. This micellar to monomer transition may involve complex thermodynamic interactions between bile salts alone or with phospholipids, i.e. mixed micelles and the aqueous environment. We therefore went on to test if therapeutically relevant changes in temperature could influence micellar behavior of bile salts, and in turn whether this affected the biological responses in cells, and in vivo. Sodium taurocholate (STC) belongs to a major class of bile salts. STC has a CMC in the 5–8 mM range and its infusion into the pancreatic duct is commonly used to study pancreatitis. We thus studied micellar breakdown of STC using isothermal titration calorimetry (ITC), dynamic light scattering and cryogenic transmission electron microscopy. Under conditions relevant to the in vivo environment (pH 7.4, Na 0.15 M), ITC showed STC to have a U shaped reduction in micellar breakdown between 37 °C and 15 °C with a nadir at 25 °C approaching ≈90% inhibition. This temperature dependence paralleled pancreatic acinar injury induced by monomeric STC. Mixed micelles of STC and 1-palmitoyl, 2-oleyl phosphatidylcholine, a phospholipid present in high proportions in bile, behaved similarly, with ≈75% reduction in micellar breakdown at 25 °C compared to 37 °C. In vivo pancreatic cooling to 25 °C reduced the increase in circulating BAs after infusion of 120 mM (5%) STC into the pancreatic duct, and duct ligation. Lower BA levels were associated with improved cardiac function, reduced myocardial damage, shock, lung injury and improved survival independent of pancreatic injury. Thus micellar breakdown of bile salts is essential for their entry into the systemic circulation, and thermodynamic interference with this may reduce their systemic entry and consequent injury during cholestasis, such as from biliary pancreatitis.
Genetic regulators of mineral amount in Nelore cattle muscle predicted by a new co-expression and regulatory impact factor approach
Authors: Afonso Juliana , Fortes Marina Rufino Salinas , Reverter Antonio , Diniz Wellison Jarles da Silva , Cesar Aline Silva Mello , Lima Andressa Oliveira de , Petrini Juliana , de Souza Marcela M. , Coutinho Luiz Lehmann , Mourão Gerson Barreto , Zerlotini Adhemar , Gromboni Caio Fernando , Nogueira Ana Rita Araújo , Regitano Luciana Correia de Almeida 
Published: May 2020

AbstractMineral contents in bovine muscle can affect meat quality, growth, health, and reproductive traits. To better understand the genetic basis of this phenotype in Nelore (Bosindicus) cattle, we analysed genome-wide mRNA and miRNA expression data from 114 muscle samples. The analysis implemented a new application for two complementary algorithms: the partial correlation and information theory (PCIT) and the regulatory impact factor (RIF), in which we included the estimated genomic breeding values (GEBVs) for the phenotypes additionally to the expression levels, originally proposed for these methods. We used PCIT to determine putative regulatory relationships based on significant associations between gene expression and GEBVs for each mineral amount. Then, RIF was adopted to determine the regulatory impact of genes and miRNAs expression over the GEBVs for the mineral amounts. We also investigated over-represented pathways, as well as pieces of evidences from previous studies carried in the same population and in the literature, to determine regulatory genes for the mineral amounts. For example,NOX1expression level was positively correlated to Zinc and has been described as Zinc-regulated in humans. Based on our approach, we were able to identify genes, miRNAs and pathways not yet described as underlying mineral amount. The results support the hypothesis that extracellular matrix interactions are the core regulator of mineral amount in muscle cells. Putative regulators described here add information to this hypothesis, expanding the knowledge on molecular relationships between gene expression and minerals.
GSEA-assisted gene signatures valid for combinations of prognostic markers in PCNSL
Authors: Takashima Yasuo , Hamano Momoko , Fukai Junya , Iwadate Yasuo , Kajiwara Koji , Kobayashi Tsutomu , Hondoh Hiroaki , Yamanaka Ryuya 
Published: May 2020

AbstractPrimary central nervous system lymphoma (PCNSL) is a brain malignant non-Hodgkin’s B-cell lymphoma. The standard treatments are high-dose methotrexate (MTX)-based chemotherapies and deferred whole brain radiotherapy. However, MTX resistance-dependent global expression and signaling pathway changes and their relationship with prognoses have not yet been elucidated. Here, we conducted a global expression analysis with next-generation sequencing and gene set enrichment analysis (GSEA) in MTX-resistant PCNSL cell lines (HKBML-MTX and TK-MTX) and PCNSL tissues. In rank scores, genes listed in HKBML-MTX and TK-MTX were enriched in PCNSL with poor prognoses. In fold changes, a part of differentially-expressed genes in PCNSL tissues were also detected in HKBML-MTX and TK-MTX cells; FOXD2-AS1 and MMP19 were commonly expressed in both HKBML-MTX and TK-MTX, FABP5 and CD70 were HKBML-MTX-specifically expressed, and CLCN2, HOXB9, INE1, and LRP5L were TK-MTX-specifically expressed, which may provide a combination of prognostic markers on MTX-sensitivities in PCNSL. Additionally, PCNSL subgroups, divided with hierarchical clustering and Kaplan-Meier methods, included twenty commonly expressed genes in both HKBML-MTX and TK-MTX, ten HKBML-MTX-specifically expressed genes, and two TK-MTX-specifically expressed genes. These results suggest that the GSEA-assisted gene signatures can provide a combination for prognostic markers in recurrent PCNSL with MTX resistances.
Arm-pull thrust in human swimming and the effect of post-activation potentiation
Authors: Barbosa Tiago M. , Yam Jia Wen , Lum Danny , Balasekaran Govindasamy , Marinho Daniel A. 
Published: May 2020

AbstractThe aim of this study was to analyse the front-crawl arm-pull kinetics and kinematics, comparing it before and after post-activation potentiation (PAP), and the associations between variables describing of the arm-pull kinetics. Twelve male competitive swimmers were randomly assigned to perform two different warm-ups in a crossover manner: (i) non-PAP (control condition); and (ii) PAP (experimental condition). PAP consisted of 2 × 5 arm-pulls with resistance bands by both upper-limbs. Eight minutes later, participants underwent a 25 m all-out trial in front-crawl arm-pull. Kinetics (i.e., peak thrust, mean thrust and thrust-time integral) and kinematics (i.e., speed and speed fluctuation) were collected by an in-house customised system composed of differential pressure sensors, speedo-meter and underwater camera. There was a significant and large improvement of the arm-pull kinetics after completing the warm-up with PAP sets (0.010 < P < 0.054, 0.50 < d < 0.74). There were non-significant and small effects of PAP on speed (P = 0.307, d = 0.18) and speed fluctuation (P = 0.498, d = 0.04). Correlation coefficients among kinetic variables were significant with large associations (0.51 < R < 0.90, 0.001 < P < 0.088). In conclusion, warm-ups including PAP conditioning sets elicit a large improvement in the thrust, but with small improvement in performance. Variables used to characterise thrust are strongly correlated and hence can be used interchangeably.
Restrictive intraoperative fluid management was associated with higher incidence of composite complications compared to less restrictive strategies in open thoracotomy: A retrospective cohort study
Authors: Kim Jie Ae , Ahn Hyun Joo , Oh Ah Ran , Choi Jisun 
Published: May 2020

AbstractRestrictive fluid management has been recommended for thoracic surgery. However, specific guidelines are lacking, and there is always concern regarding impairment of renal perfusion with a restrictive policy. The objective of this study was to find the net intraoperative fluid infusion rate which shows the lowest incidence of composite complications (either pulmonary complications or acute kidney injury) in open thoracotomy. We hypothesized that a certain range of infusion rate would decrease the composite complications within postoperative 30 days. All patients (n = 1,031) who underwent open thoracotomy at a tertiary care university hospital were included in this retrospective study. The time frame of fluid monitoring was from the start of operation to postoperative 24 hours. The cutoff value of the intraoperative net fluid amount was 4–5 ml.kg−1.h−1 according to the minimum p-value method, thus, patients were divided into Low (≤3 ml.kg−1.h−1), Cutoff (4–5 ml.kg−1.h−1) and High (≥6 ml.kg−1.h−1) groups. The Cutoff group showed the lowest composite complication rate (19%, 12%, and 13% in the Low, Cutoff, and High groups, respectively, P = 0.0283; Low vs. Cutoff, P = 0.0324, Bonferroni correction). Acute respiratory distress syndrome occurred least frequently in the Cutoff group (7%, 3%, and 6% for the Low, Cutoff, and High groups, respectively, P = 0.0467; Low vs. Cutoff, P = 0.0432, Bonferroni correction). In multivariable analysis, intraoperative net fluid infusion rate was associated with composite complications, and the Cutoff group decreased risk (odds ratio 0.54, 95% confidence interval: 0.35–0.81, P = 0.0035). In conclusion, maintaining intraoperative net fluid infusion at 4–5 ml.kg−1.h−1 was associated with better results in open thoracotomy, in terms of composite complications, compared to more restrictive fluid management.
Evaluation of an augmented reality platform for austere surgical telementoring: a randomized controlled crossover study in cricothyroidotomies
Authors: Rojas-Muñoz Edgar , Lin Chengyuan , Sanchez-Tamayo Natalia , Cabrera Maria Eugenia , Andersen Daniel , Popescu Voicu , Barragan Juan Antonio , Zarzaur Ben , Murphy Patrick , Anderson Kathryn , Douglas Thomas , Griffis Clare , McKee Jessica , Kirkpatrick Andrew W. , Wachs Juan P. 
Published: May 2020

AbstractTelementoring platforms can help transfer surgical expertise remotely. However, most telementoring platforms are not designed to assist in austere, pre-hospital settings. This paper evaluates the system for telementoring with augmented reality (STAR), a portable and self-contained telementoring platform based on an augmented reality head-mounted display (ARHMD). The system is designed to assist in austere scenarios: a stabilized first-person view of the operating field is sent to a remote expert, who creates surgical instructions that a local first responder wearing the ARHMD can visualize as three-dimensional models projected onto the patient’s body. Our hypothesis evaluated whether remote guidance with STAR could lead to performing a surgical procedure better, as opposed to remote audio-only guidance. Remote expert surgeons guided first responders through training cricothyroidotomies in a simulated austere scenario, and on-site surgeons evaluated the participants using standardized evaluation tools. The evaluation comprehended completion time and technique performance of specific cricothyroidotomy steps. The analyses were also performed considering the participants’ years of experience as first responders, and their experience performing cricothyroidotomies. A linear mixed model analysis showed that using STAR was associated with higher procedural and non-procedural scores, and overall better performance. Additionally, a binary logistic regression analysis showed that using STAR was associated to safer and more successful executions of cricothyroidotomies. This work demonstrates that remote mentors can use STAR to provide first responders with guidance and surgical knowledge, and represents a first step towards the adoption of ARHMDs to convey clinical expertise remotely in austere scenarios.
Active metabolism unmasks functional protein–protein interactions in real time in-cell NMR
Authors: Breindel Leonard , Burz David S. , Shekhtman Alexander 
Published: May 2020

AbstractProtein–protein interactions, PPIs, underlie most cellular processes, but many PPIs depend on a particular metabolic state that can only be observed in live, actively metabolizing cells. Real time in-cell NMR spectroscopy, RT-NMR, utilizes a bioreactor to maintain cells in an active metabolic state. Improvement in bioreactor technology maintains ATP levels at >95% for up to 24 hours, enabling protein overexpression and a previously undetected interaction between prokaryotic ubiquitin-like protein, Pup, and mycobacterial proteasomal ATPase, Mpa, to be detected. Singular value decomposition, SVD, of the NMR spectra collected over the course of Mpa overexpression easily identified the PPIs despite the large variation in background signals due to the highly active metabolome.
Pentraxin 3 promotes the osteoblastic differentiation of MC3T3-E1 cells through the PI3K/Akt signaling pathway
Authors: Liu Yong , Wang Hui , Zhou Xiao-zhe , Li Ning , Guo Yi-chao , Chen Tao-ping 
Published: May 2020

Abstract
Osteoblast cells are responsible for synthesizing new bone tissue, and determining how to control osteoblastic differentiation is vital to the treatment of osteoporosis. In the present study, we show that pentraxin 3 (PTX3) signaling is involved in the regulation of osteoblastic differentiation in MC3T3-E1 cells. Our data reveal that PTX3 is abundantly expressed in MC3T3-E1 cells and that its expression is inducible by the introduction of osteogenic induction medium (OIM). Overexpression of PTX3 was observed to significantly increase the expression of four osteoblast signature genes, including Runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteocalcin (OCN) and osterix (OSX), suggesting that the overexpression of PTX3 promotes osteoblastic differentiation. The relative level of gene expression between OIM and OIM plus overexpressed PTX3 was evaluated using the Affymetrix Gene Chip® mouse gene microarray. PTX3-related differentially expressed genes (DEGs) were screened. Gene ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes database (KEGG) pathway enrichment analyses were performed, and the PI3K/Akt signaling pathway was primarily involved in the osteogenic differentiation of PTX3. Protein–protein interactions (PPIs) were also constructed, and the molecular complex detection (MCODE) plugin calculated modules of PPI networks. Moreover, we show that the effect of PTX3 is mediated by its induction of the PI3K/Akt signaling pathway. Mechanistically, we show that the action of PTX3 requires the activation of PI3K and Akt, and deactivation of PI3K by its inhibitor LY294002 weakens the PTX3-mediated induction of osteoblast signature genes, ALP and matrix mineralization. The present study revealed a new role played by PTX3 and suggest a potential mechanism governing the osteoblastic differentiation of MC3T3-E1 cells.
Clinical Implementation of Transcranial Direct Current Stimulation in Aphasia: A Survey of Speech-Language Pathologists
Authors: Keator Lynsey M. , Basilakos Alexandra , Rorden Christopher , Elm Jordan , Bonilha Leonardo , Fridriksson Julius 
Published: May 2020

PurposeThe objectives of this study are to (a) identify speech-language pathologists' (SLPs') familiarity with transcranial direct current stimulation (tDCS), (b) quantify what SLPs consider necessary tDCS-related improvement in aphasia severity (i.e., tDCS enhancement; desired improvement above and beyond traditional behavioral therapy) to implement this adjuvant therapy for the clinical management of aphasia, and (c) identify concerns that could potentially hinder the clinical adoption of tDCS.MethodA brief (14-question) survey was disseminated via e-mail and social media outlets targeting SLPs working with individuals with aphasia.ResultsTwo hundred twenty-one individuals responded, and 155 valid surveys were analyzed. Seventy-one percent of participants reported familiarity with tDCS prior to taking the survey. Clinicians reported a desired mean enhancement of 22.9% additional points on the Western Aphasia Battery–Revised Aphasia Quotient. Importantly, 94.2% of SLPs reported concerns regarding the implementation of tDCS in clinical settings (i.e., safety, cost, administrative approval, reimbursement and training).ConclusionsThis is the first study to identify SLPs' perspectives regarding the clinical adoption of tDCS. Results suggest the majority of queried SLPs were familiar with tDCS prior to taking the survey. Although SLPs report a desired improvement of approximately 23% additional points on the Western Aphasia Battery–Revised Aphasia Quotient to consider adopting tDCS into practice, many SLPs reported concerns regarding clinical adoption. Responses from the current survey offer important preliminary evidence to begin bridging the research-to-practice gap as it relates to the clinical implementation of tDCS. Relatedly, these results will inform future clinical trials.
Detection of exomoons in simulated light curves with a regularized convolutional neural network
Authors: Alshehhi Rasha , Rodenbeck Kai , Gizon Laurent , Sreenivasan Katepalli R. 
Published: May 2020

Context.Many moons have been detected around planets in our Solar System, but none has been detected unambiguously around any of the confirmed extrasolar planets.Aims.We test the feasibility of a supervised convolutional neural network to classify photometric transit light curves of planet-host stars and identify exomoon transits, while avoiding false positives caused by stellar variability or instrumental noise.Methods.Convolutional neural networks are known to have contributed to improving the accuracy of classification tasks. The network optimization is typically performed without studying the effect of noise on the training process. Here we design and optimize a 1D convolutional neural network to classify photometric transit light curves. We regularize the network by the total variation loss in order to remove unwanted variations in the data features.Results.Using numerical experiments, we demonstrate the benefits of our network, which produces results comparable to or better than the standard network solutions. Most importantly, our network clearly outperforms a classical method used in exoplanet science to identify moon-like signals. Thus the proposed network is a promising approach for analyzing real transit light curves in the future.