bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bactericidal efficacy of meropenem in combination with cefmetazole against IMP-producing carbapenem-resistant Enterobacteriaceae
Authors: Abe Ryuichiro , Hagiya Hideharu , Akeda Yukihiro , Yamamoto Norihisa , Ishii Yoshikazu , Tomono Kazunori 
Published: November 2019

Abstract

Objective
Carbapenem-resistant Enterobacteriaceae (CRE) are among the most severe threats to public and clinical health because of their high levels of resistance to various antibiotics. We assessed the efficacy of combination therapy with meropenem (MEM) and cefmetazole (CMZ) against Imipenemase (IMP)-producing CRE, using the checkerboard method and time-killing assay on 13 Enterobacteriaceae isolates harboring blaIMP-1 (4 Enterobacter hormaechei, 5 Escherichia coli, and 4 Klebsiella pneumoniae isolates) and 13 isolates harboring blaIMP-6 (8 E. coli and 5 K. pneumoniae isolates).

Results
Minimum inhibitory concentrations (MICs) of MEM and CMZ ranged from 2 to 64 and 64 to 2048 μg/mL, respectively. Checkerboard method demonstrated the synergy of the MEM/CMZ combination in all the tested IMP-producing CRE isolates, and the time-kill assay indicated a bactericidal effect for both blaIMP-1 and blaIMP-6 positive CRE when MEM/CMZ combination was used. In vitro, the MEM/CMZ combination was potentially effective against IMP-1- or IMP-6-producing CRE. Further investigations including in vivo animal studies and clinical studies are warranted to corroborate the clinical utility of the novel combination therapy.
An exploration of the rapid transformation method for Dunaliella salina system
Authors: Song Guannan , Wang Wan , Hu Lina , Liu Yu , Li Aifang , Du Jingxia , Wang Jiao , Jia Mengyuan , Feng Shuying 
Published: November 2019

Abstract
As a new expression system, Dunaliella salina (D. salina) has bright prospects and applications in various fields. However, its application is currently restricted because of the low expression and instability of foreign gene in D. salina cells. During genetic operation, transformation is a crucial step for genes expression in D. salina system. Although several transformation methods are existing currently, many inherent deficiencies and limitations still can be found in actual practice. Thus, we attempted to set up a rapid transformation method using the change of salt concentrations for D. salina. Based on osmotic pressure difference, exogenous genes can be spontaneously transferred into D. salina cells. After that, transformed D. salina cells were subjected to histochemical and molecular analysis. The results showed that the reporter gene, beta-glucuronidase genes were successfully expressed in the positive transformants, and detected in all of transformed cells by PCR analysis. Moreover, different transformation parameters, containing the salt gradient, time, dye dosage and Triton X-100 concentration, were optimized to obtain an optimal transformation result. Taken together, we preliminarily established a rapid transformation method with the features of fast, simple, economic, and high-efficient. This method will provide a strong genetic manipulation tool for the future transformation of D. salina system.
Healthcare trajectories before and after critical illness: population-based insight on diverse patients clusters
Authors: Jouan Youenn , Grammatico-Guillon Leslie , Teixera Noémie , Hassen-Khodja Claire , Gaborit Christophe , Salmon-Gandonnière Charlotte , Guillon Antoine , Ehrmann Stephan 
Published: November 2019

Abstract

Background
The post intensive care syndrome (PICS) gathers various disabilities, associated with a substantial healthcare use. However, patients’ comorbidities and active medical conditions prior to intensive care unit (ICU) admission may partly drive healthcare use after ICU discharge. To better understand retative contribution of critical illness and PICS—compared to pre-existing comorbidities—as potential determinant of post-critical illness healthcare use, we conducted a population-based evaluation of patients’ healthcare use trajectories.

Results
Using discharge databases in a 2.5-million-people region in France, we retrieved, over 3 years, all adult patients admitted in ICU for septic shock or acute respiratory distress syndrome (ARDS), intubated at least 5 days and discharged alive from hospital: 882 patients were included. Median duration of mechanical ventilation was 11 days (interquartile ranges [IQR] 8;20), mean SAPS2 was 49, and median hospital length of stay was 42 days (IQR 29;64). Healthcare use (days spent in healthcare facilities) was analyzed 2 years before and 2 years after ICU admission. Prior to ICU admission, we observed, at the scale of the whole study population, a progressive increase in healthcare use. Healthcare trajectories were then explored at individual level, and patients were assembled according to their individual pre-ICU healthcare use trajectory by clusterization with the K-Means method. Interestingly, this revealed diverse trajectories, identifying patients with elevated and increasing healthcare use (n = 126), and two main groups with low (n = 476) or no (n = 251) pre-ICU healthcare use. In ICU, however, SAPS2, duration of mechanical ventilation and length of stay were not different across the groups. Analysis of post-ICU healthcare trajectories for each group revealed that patients with low or no pre-ICU healthcare (which represented 83% of the population) switched to a persistent and elevated healthcare use during the 2 years post-ICU.

Conclusion
For 83% of ARDS/septic shock survivors, critical illness appears to have a pivotal role in healthcare trajectories, with a switch from a low and stable healthcare use prior to ICU to a sustained higher healthcare recourse 2 years after ICU discharge. This underpins the hypothesis of long-term critical illness and PICS-related quantifiable consequences in healthcare use, measurable at a population level.
Optimal two-parameter geometric and arithmetic mean bounds for the Sándor–Yang mean
Authors: Qian Wei-Mao , Yang Yue-Ying , Zhang Hong-Wei , Chu Yu-Ming 
Published: November 2019

Abstract
In the article, we provide the sharp bounds for the Sándor–Yang mean in terms of certain families of the two-parameter geometric and arithmetic mean and the one-parameter geometric and harmonic means. As applications, we present new bounds for a certain Yang mean and the inverse tangent function.
The partially shared values and small functions for meromorphic functions in a k-punctured complex plane
Authors: Xu Hong Yan , Li Yong Ming , Liu Shan 
Published: November 2019

Abstract
The main aim of this article is to discuss the uniqueness of meromorphic functions partially sharing some values and small functions in a k-punctured complex plane Ω. We proved the following: Let $f_{1},f_{2}$f1,f2 be two admissible meromorphic functions in Ω and $\alpha _{j}\ (j=1,2,\ldots ,l)$αj(j=1,2,…,l) be $l(\geq 5)$l(≥5) distinct small functions with respect to f and g. If $\widetilde{E}(\alpha _{j},\varOmega ,f_{1})\subseteq \widetilde{E}(\alpha _{j},\varOmega , f_{2})\ (j=1,2,\ldots ,l)$E˜(αj,Ω,f1)⊆E˜(αj,Ω,f2)(j=1,2,…,l) and
$$ \liminf_{r\rightarrow +\infty }\frac{\sum_{j=1}^{l}\overline{N} _{0} (r,\frac{1}{f_{1}-\alpha _{j}} )}{\sum_{j=1} ^{l}\overline{N}_{0} (r,\frac{1}{f_{2}-\alpha _{j}} )}> \frac{5}{2l-5}, $$lim infr→+∞∑j=1lN‾0(r,1f1−αj)∑j=1lN‾0(r,1f2−αj)>52l−5, then $f_{1}\equiv f_{2}$f1≡f2. Our results are some improvements and extension of previous theorems given by Cao–Yi and Ge–Wu.
Efficacy of medium-chain fatty acid salts distilled from coconut oil against two enteric pathogen challenges in weanling piglets
Authors: López-Colom Paola , Castillejos Lorena , Rodríguez-Sorrento Agustina , Puyalto Mónica , Mallo Juan José , Martín-Orúe Susana María 
Published: November 2019

Abstract

Background
The search for alternatives to antibiotics in pig production has increased the interest in natural resources with antimicrobial properties, such as medium-chain fatty acids (MCFA) as in-feed additives. This study evaluated the potential of a novel blend of MCFA salts (DIC) from distilled coconut oil with a lauric acid content to reduce enteropathogens and control intestinal diseases around weaning. Two experimental disease models were implemented in early-weaned piglets, consisting of two oral challenges: Salmonella Typhimurium (1.2 × 108 CFU) or enterotoxigenic Escherichia coli (ETEC) F4 (1.5 × 109 CFU). The parameters assessed were: animal performance, clinical signs, pathogen excretion, intestinal fermentation, immune-inflammatory response, and intestinal morphology.

Results
The Salmonella challenge promoted an acute course of diarrhea, with most of the parameters responding to the challenge, whereas the ETEC F4 challenge promoted a mild clinical course. A consistent antipathogenic effect of DIC was observed in both trials in the hindgut, with reductions in Salmonella spp. plate counts in the cecum (P = 0.03) on d 8 post-inoculation (PI) (Salmonella trial), and of enterobacteria and total coliform counts in the ileum and colon (P < 0.10) on d 8 PI (ETEC F4 trial). When analyzing the entire colonic microbiota (16S rRNA gene sequencing), this additive tended (P = 0.13) to reduce the Firmicutes/Bacteroidetes ratio and enriched Fibrobacteres after the Salmonella challenge. In the ETEC F4 challenge, DIC prompted structural changes in the ecosystem with increases in Dialister, and a trend (P = 0.14) to increase the Veillonellaceae family. Other parameters such as the intestinal fermentation products or serum pro-inflammatory mediators were not modified by DIC supplementation, nor were the histological parameters. Only the intraepithelial lymphocyte (IEL) counts were lowered by DIC in animals challenged with Salmonella (P = 0.07). With ETEC F4, the IEL counts were higher with DIC on d 8 PI (P = 0.08).

Conclusions
This study confirms the potential activity of this MCFA salts mixture to reduce intestinal colonization by opportunistic pathogens such as Salmonella or E. coli and its ability to modulate colonic microbiota. These changes could explain to some extent the local immune cell response at the ileal level.
Resonant Kuiper belt objects: a review
Authors: Malhotra Renu 
Published: November 2019

Abstract
Our understanding of the history of the solar system has undergone a revolution in recent years, owing to new theoretical insights into the origin of Pluto and the discovery of the Kuiper belt and its rich dynamical structure. The emerging picture of dramatic orbital migration of the planets driven by interaction with the primordial Kuiper belt is thought to have produced the final solar system architecture that we live in today. This paper gives a brief summary of this new view of our solar system’s history and reviews the astronomical evidence in the resonant populations of the Kuiper belt.
Old nest material functions as an informative cue in making nest-site selection decisions in the European Kestrel (Falco tinnunculus)
Authors: E Mingju , Wang Tuo , Wang Shangyu , Gong Ye , Yu Jiangping , Wang Lin , Ou Wei , Wang Haitao 
Published: November 2019

Abstract

Background
For secondary cavity-nesting bird species that do not add lining materials to nests, the presence of old nest material or organic remains that have accumulated within nest cavities from previous breeding events may be a cue of nest-site quality. These materials potentially contain information about past breeding success in con- and heterospecifics and may improve the thermal insulation of eggs during incubation. However, few studies have addressed whether the presence of old nest materials serves as a cue for cavity-nesting raptors when choosing specific nest sites.

Methods
We conducted a 9-year nest box experiment to test whether old nest materials from con- and heterospecifics serve as informative cues to the European Kestrel (Falco tinnunculus) when making nest selection decisions, as this species uses nest boxes without adding nesting material.

Results
The presence of old nest materials and entrance size best discriminated nest boxes occupied by European Kestrels from unoccupied boxes. Nest boxes containing conspecific organic remains, artificial dry leaf and branch material, and material left behind by Great Tits (Parus major) were reused at higher rates, especially those containing conspecific nest material, than nest boxes containing true or simulated nest materials from predators. In 2010, no single nest box was occupied by the same banded individual that occupied the box in the previous year (10 females and 2 males were banded in 2009).

Conclusions
European Kestrels preferred nest boxes containing old nest material over empty boxes, which is consistent with previous findings that they exploit con- and heterospecific cues when deciding where to settle and breed, as old nest or organic material provides substrate for incubating females. Kestrels may be able to assess the predation risks associated with a specific nest site based on experience or the presence of prey remains. The repeated use of nest boxes across breeding seasons by kestrels cannot be entirely ascribed to philopatry. This study provides evidence that old nest materials are potentially used as informative cues when making nest-site selection decisions in European Kestrels.
Functional exploration of co-expression networks identifies a nexus for modulating protein and citric acid titres in Aspergillus niger submerged culture
Authors: Cairns Timothy C. , Feurstein Claudia , Zheng Xiaomei , Zhang Li Hui , Zheng Ping , Sun Jibin , Meyer Vera 
Published: November 2019

Abstract

Background
Filamentous fungal cell factories are used to produce numerous proteins, enzymes, and organic acids. Protein secretion and filamentous growth are tightly coupled at the hyphal tip. Additionally, both these processes require ATP and amino acid precursors derived from the citric acid cycle. Despite this interconnection of organic acid production and protein secretion/filamentous growth, few studies in fungi have identified genes which may concomitantly impact all three processes.

Results
We applied a novel screen of a global co-expression network in the cell factory Aspergillus niger to identify candidate genes which may concomitantly impact macromorphology, and protein/organic acid fermentation. This identified genes predicted to encode the Golgi localized ArfA GTPase activating protein (GAP, AgeB), and ArfA guanine nucleotide exchange factors (GEFs SecG and GeaB) to be co-expressed with citric acid cycle genes. Consequently, we used CRISPR-based genome editing to place the titratable Tet-on expression system upstream of ageB, secG, and geaB in A. niger. Functional analysis revealed that ageB and geaB are essential whereas secG was dispensable for early filamentous growth. Next, gene expression was titrated during submerged cultivations under conditions for either protein or organic acid production. ArfA regulators played varied and culture-dependent roles on pellet formation. Notably, ageB or geaB expression levels had major impacts on protein secretion, whereas secG was dispensable. In contrast, reduced expression of each predicted ArfA regulator resulted in an absence of citric acid in growth media. Finally, titrated expression of either GEFs resulted in an increase in oxaloacetic acid concentrations in supernatants.

Conclusion
Our data suggest that the Golgi may play an underappreciated role in modulating organic acid titres during industrial applications, and that this is SecG, GeaB and AgeB dependent in A. niger. These data may lead to novel avenues for strain optimization in filamentous fungi for improved protein and organic acid titres.
Smoothness: an Unexplored Window into Coordinated Running Proficiency
Authors: Kiely John , Pickering Craig , Collins David J. 
Published: November 2019

Abstract
Over the expanse of evolutionary history, humans, and predecessor Homo species, ran to survive. This legacy is reflected in many deeply and irrevocably embedded neurological and biological design features, features which shape how we run, yet were themselves shaped by running.
Smoothness is a widely recognised feature of healthy, proficient movement. Nevertheless, although the term ‘smoothness’ is commonly used to describe skilled athletic movement within practical sporting contexts, it is rarely specifically defined, is rarely quantified and remains barely explored experimentally. Elsewhere, however, within various health-related and neuro-physiological domains, many manifestations of movement smoothness have been extensively investigated. Within this literature, smoothness is considered a reflection of a healthy central nervous system (CNS) and is implicitly associated with practiced coordinated proficiency; ‘non-smooth’ movement, in contrast, is considered a consequence of pathological, un-practiced or otherwise inhibited motor control.
Despite the ubiquity of running across human cultures, however, and the apparent importance of smoothness as a fundamental feature of healthy movement control, to date, no theoretical framework linking the phenomenon of movement smoothness to running proficiency has been proposed. Such a framework could, however, provide a novel lens through which to contextualise the deep underlying nature of coordinated running control. Here, we consider the relevant evidence and suggest how running smoothness may integrate with other related concepts such as complexity, entropy and variability. Finally, we suggest that these insights may provide new means of coherently conceptualising running coordination, may guide future research directions, and may productively inform practical coaching philosophies.
A feasibility cluster randomised controlled trial of a preschool obesity prevention intervention: ToyBox-Scotland
Authors: Malden Stephen , Reilly John. J. , Gibson Ann-Marie , Bardid Farid , Summerbell Carolyn , De Craemer Marieke , Cardon Greet , Androutsos Odysseas , Manios Yannis , Hughes Adrienne 
Published: November 2019

Abstract

Background
High levels of childhood obesity have been observed globally over the last three decades. Preschools are promising settings to implement obesity prevention interventions in the early years. The aim of this study was to test the feasibility of a cluster randomised controlled trial of the ToyBox-Scotland preschool obesity prevention intervention.

Methods
Six preschools in predominantly deprived areas of Glasgow, UK, were randomised to either the ToyBox intervention (n = 3) or usual curriculum control group (n = 3). The intervention ran for 18 weeks from March–June 2018, and consisted of practitioner-led physical activity and sedentary behaviour sessions in preschools, with an additional interactive home component. Primary outcome measures were intervention fidelity, recruitment rates, attrition rates, and compliance with trial procedures. Secondary outcomes were body mass index (BMI) z-score, bioelectrical impedance analysis (BIA), objectively measured physical activity and sedentary time via activPAL accelerometer, and parent-reported home eating, snacking, and water consumption.

Results
The preschool component of the intervention was implemented with high fidelity (64%), while the home component was implemented with low fidelity (41%). A cluster-level recruitment rate of 10% was achieved, and the individual-level recruitment rate was 18% (42/233 children, mean age 4.4 years; 17 girls). The attrition rate was 14%, and compliance rates varied considerably by the outcome. Compliance was highest for BMI (86%), while 19% of the sample returned valid accelerometer data for both baseline and follow-up and the parental questionnaire response rate was 23%. Both intervention and control groups showed small increases in BMI z-scores at follow-up of 0.02 and 0.06, respectively. Both groups had small decreases in physical activity and increases in sedentary time at follow-up.

Conclusions
Before progression to an effectiveness trial, additional procedures should be considered to improve recruitment rates, compliance with outcome measures, and implementation of the home-based component of the ToyBox-Scotland intervention.

Trial registration
ISRCTN12831555
An analysis of passengers’ ride needs of urban rail transit services: application of quality function deployment
Authors: Chan Catherine Y. P. , Chin Kwai-Sang , Chan Candace , Tsui Kwok L. 
Published: November 2019

Abstract
This study aims to suggest using the interpretation type of deployment of quality function deployment (QFD) as a methodology to understand the passengers’ needs when they situate in the train cars of urban rail, using the East Rail Line (ERL) in Hong Kong as a case study. The result shows that behaved environment and safe stand are the two most important ride needs demanded by the everyday ordinary passengers of the rail line. In addition to this, the members of the study team reflected that the deployment process has greatly helped them on acquiring a thorough understanding of the passengers’ needs, which is very essential for deriving innovative solutions. Besides the rail company could make use of the case study for improving its train ride quality, the suggested methodology is applicable to understand the passengers’ ride needs of other kinds of public transport as well as the recipient needs of those public services that are of similar nature.
Preanesthetic severe postural hypotension following 5-aminolevulinic acid pretreatment in patients for photodynamic diagnosis-assisted urological surgery
Authors: Shiratori Tohru , Hotta Kunihisa , Satoh Masaaki , Kiuchi Chiaki , Ogawa Noriyuki , Kamigaito Takayuki 
Published: November 2019

Abstract

Background
5-Aminolevulinic acid (5-ALA) is utilized for photodynamic diagnosis-assisted (PDD) surgery. However, it has been associated with vasodilation, hence, occasional hypotension.

Case presentation
We encountered two patients who had severe postural hypotension following 5-ALA pretreatment prior to an operation. They were scheduled for urological PDD surgery, but upon standing to walk to the operation room, they felt sick because of severe hypotension. One of them underwent the surgery after recovery, but the other surgery was canceled due to a prolonged hypotension that lasted for more than a day.

Conclusions
Severe postural hypotension may develop as a result of the high concentration of porphyrin precursors, which may affect the nervous system. Severe postural hypotension may be due to 5-ALA-induced autonomic dysfunction as well as vasodilative action of 5-ALA. These observations suggest that in addition to the careful monitoring of patients’ vital signs, standing should be avoided following 5-ALA pretreatment.