bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical utility of polyethylene glycol conjugated granulocyte colony-stimulating factor (PEG-G-CSF) for preventing severe neutropenia in metastatic colorectal cancer patients treated with FOLFOXIRI plus bevacizumab: A single-center retrospective study
Authors: Yusuke Kitagawa , Osumi Hiroki , Shinozaki Eiji , Ota Yumiko , Nakayama Izuma , Suzuki Takeshi , Wakatsuki Takeru , Ogura Mariko , Ooki Akira , Takahari Daisuke , Suenaga Mitsukuni , Chin Keisho , Yamaguchi Kensei 
Published: February 2020

Abstract
Background: This study aimed to evaluate the efficacy and safety of polyethylene glycol conjugated granulocyte colony-stimulating factor (PEG-G-CSF) for preventing neutropenia in metastatic colorectal cancer (mCRC) patients that received fluorouracil, leucovorin, oxaliplatin, and irinotecan (FOLFOXIRI) plus bevacizumab (Bev) in clinical practice. Methods: We retrospectively analyzed mCRC patients who received FOLFOXIRI plus Bev between December 2015 and December 2017. We evaluated the efficacy of PEG-G-CSF as preventing or treating grade 3/4 neutropenia, the overall response rate (ORR) according to the Response Evaluation Criteria in Solid Tumors version 1.1, progression-free survival (PFS), overall survival (OS), and adverse events of FOLFOXIRI plus Bev based on the Common Terminology Criteria for Adverse Events version 4.0. Results A total of 26 patients (median age 53.5 years) were included. The ORR rate was 65.3%, the median PFS was 9.6 months (7.2–16.9), and the median OS was 24.2 months (13.6–NA). Grade 3 or 4 neutropenia occurred in 53.8% of the patients, and febrile neutropenia occurred in 7.7%. PEG-G-CSF was given to 77.0% of the patients, including prophylactically (n = 9) and after the development of grade 3 or 4 neutropenia (n = 11). No patients experienced grade 3 or higher neutropenia after the administration of PEG-G-CSF. In seven of the nine patients who received PEG-G-CSF prophylactically (77.8%), no dose adjustment was required. Conclusions PEG-G-CSF is useful in preventing severe neutropenia in mCRC patients treated with FOLFOXIRI plus Bev.
Pneumocystis pneumonia occurrence and prophylaxis duration in kidney transplant recipients according to perioperative treatment with rituximab: a single-center, retrospective study
Authors: Kim Young Hoon , Kim Jee Yeon , Kim Dong Hyun , Ko Youngmin , Choi Ji Yoon , Shin Sung , Jung Joo Hee , Park Su-Kil , Kim Sung-Han , Kwon Hyunwook , Han Duck Jong 
Published: February 2020

Abstract
Background

Pneumocystis pneumonia (PCP) is a life-threatening fungal infection that can occur in kidney transplantation (KT) recipients. A growing number of KT recipients are receiving perioperative treatment with rituximab, which is associated with prolonged B-cell depletion and possible risk of PCP occurrence; however, the optimal prophylaxis duration according to rituximab treatment is yet unknown. We compared the occurrence of PCP and the duration of prophylaxis in KT recipients according to rituximab treatment.

Methods

We retrospectively analyzed 2110 patients who underwent KT between January 2009 and December 2016, who were divided into non-Rituximab group (n = 1588, 75.3%) and rituximab group (n = 522, 24.7%).

Results

In the rituximab group, the estimated number needed to treat (NNT) for prophylaxis prolongation from 6 to 12 months was 29.0 with a relative risk reduction of 90.0%. In the non-rituximab group, the estimated NNT value was 133.3 and the relative risk reduction was 66.4%. Rituximab treatment (hazard ratio (HR) = 3.09; P < 0.01) and acute rejection (HR = 2.19; P = 0.03) were significant risk factors for PCP in multivariate analysis.

Conclusions

Our results suggest that maintaining PCP prophylaxis for 12 months may be beneficial in KT recipients treated with rituximab for desensitization or acute rejection treatment.
First evidence of the deletion in the Pfhrp2 and Pfhrp3 genes in Plasmodium falciparum from Equatorial Guinea
Authors: Berzosa Pedro , González V , Taravillo L , Mayor A , Romay-Barja M , Garcia L , Ncogo P , Riloha M , Benito Agustín 
Published: February 2020

Abstract
Background

The World Health Organization (WHO) recommends rapid diagnostic tests (RDTs) as a good alternative malaria-diagnosis method in remote parts of sub-Saharan Africa. The majority of commercial RDTs currently available detect the Plasmodium falciparum protein histidine-rich protein 2 (PfHRP2). There have also been recent reports of pfhrp2 gene deletions being found in parasites collected from several African countries. The WHO has concluded that lacking the pfhrp2 gene must be monitored in Africa. The purpose of the study was to analyse why the samples that were positive by PCR were negative by RDTs and, therefore, to determine whether there have been deletions in the pfhrp2 and/or pfhrp3 genes.

Methods

Malaria NM-PCR was carried out on all the samples collected in the field. A group of 128 samples was positive by PCR but negative by RDT; these samples were classified as RDT false-negatives. PCR was carried out for exon2 of pfhrp2 and pfhrp3 genes to detect the presence or absence of these two genes. Frequencies with 95% confidence intervals (CIs) were used for prevalence estimates. Associations were assessed by the Chi-square test or Fisher´s exact test. The level of significance was set at p ≤0.05. Statistical analyses were performed using the software package SPSSv.15.0.

Results

After PCR, 81 samples were identified (4.7%, 95% CI: 3.8-5.8) which had deletion in both genes, pfhrp2 and pfhrp3 . Overall, however, 11 samples (0.6%, 95% CI: 0.36-1.14) had deletion only in pfhrp2 but not in pfhrp3 , and 15 (0.9%, 95% CI: 0.6-1.5) presented with deletion only in pfhrp3 but not in pfhrp2 . Considering the pfhrp2 gene separately, within the total of 1,724 samples, 92 (5.3%, 95% CI: 4.37-6.5) had evidence of deletion.

Conclusion

The present study provides the first evidence of deletion in the pfhrp2 and pfhrp3 genes in P. falciparum isolates from Equatorial Guinea. However, larger studies across different regions within the country and across different seasonal profiles are needed to determine the full extent of pfhrp2 and pfhrp3 deletion. It is strongly recommended to implement an active surveillance programme in order to detect any increases in pfhrp2 and pfhrp3 deletion frequencies.
Profiling the urinary microbiome in men with calcium-based kidney stones
Authors: Xie Jing , Huang Jian-sheng , Huang Xiang-jiang , Peng Jun-ming , Yu Zhou , Yuan Ye-qing , Xiao Ke-feng , Guo Ji-nan 
Published: February 2020

Abstract
Background

The dogma that urine is sterile in healthy individuals has been overturned by recent studies applying molecular-based methods. Mounting evidences indicate that dysbiosis of the urinary microbiota is associated with several urological diseases. In this study, we aimed to investigate the characteristics of the urinary microbiome of patients with calcium-based kidney stones and compare it with those of healthy individuals.

Method

We collected bladder urine samples from 22 adult male patients with calcium-based kidney stones and 21 age-matched healthy controls by transurethral catheterization. For kidney stone patients, paired renal pelvis urine was also collected via ureteral catheter using aseptic technique. The V3-V4 region of the bacterial 16S rRNA gene was sequenced, and the urinary microbiota was analyzed by kidney stone status and specimen-type.

Results

The diversity of the urinary microbiota in kidney stone patients was significantly lower than that of healthy controls based on the Shannon and Simpson index (P<0.001 for both indices). The urinary microbiota structure also significantly differed between kidney stone patients and healthy controls (ANOSIM, R = 0.11, P <0.001). Differential representation of inflammation associated bacteria (e.g., Acinetobacter ) and several enriched functional pathways were identified in the urine of kidney stones patients. Meanwhile, we found the species diversity, overall composition of microbiota and predicted functional pathways were similar between bladder urine and renal pelvis urine in kidney stone patients.

Conclusion

A marked dysbiosis of urinary microbiota in male patients with calcium-based kidney stones was observed, which may be helpful to interpret the association between bacteria and calcium-based kidney stones.
Transcriptome and metabolite analyses provide insights into zigzag-shaped stem formation in tea plants (Camellia sinensis)
Authors: Cao Hongli , Wang Feiquan , Lin Hongzheng , Ye Yijun , Zheng Yucheng , Li Jiamin , Hao Zhilong , Ye Naixing , Yue Chuan 
Published: February 2020

Abstract
Background: Shoot orientation is important for plant architecture formation, and zigzag-shaped shoots are a special trait found in many plants. Zigzag-shaped shoots have been selected and thoroughly studied in Arabidopsis; however, the regulatory mechanism underlying zigzag-shaped shoot development in other plants, especially woody plants, is largely unknown.
Results: In this study, tea plants with zigzag-shaped shoots, namely, Qiqu (QQ) and Lianyuanqiqu (LYQQ), were investigated and compared with the erect-shoot tea plant Meizhan (MZ) in an attempt to reveal the regulation of zigzag-shaped shoot formation. Tissue section observation showed that the cell arrangement and shape of zigzag-shaped stems were aberrant compared with those of normal shoots. Moreover, a total of 2175 differentially expressed genes (DEGs) were identified from the zigzag-shaped shoots of the tea plants QQ and LYQQ compared to the shoots of MZ using transcriptome sequencing, and the DEGs involved in the “Plant-pathogen interaction”, “Phenylpropanoid biosynthesis”, “Flavonoid biosynthesis” and “Linoleic acid metabolism” pathways were significantly enriched. Additionally, the DEGs associated with cell expansion, vesicular trafficking, phytohormones, and transcription factors were identified and analysed. Metabolomic analysis showed that 13 metabolites overlapped and were significantly changed in the shoots of QQ and LYQQ compared to MZ.
Conclusions: Our results suggest that zigzag-shaped shoot formation might be associated with the gravitropism response and polar auxin transport in tea plants. This study provides a valuable foundation for further understanding the regulation of plant architecture formation and for the cultivation and application of horticultural plants in the future.
Continuous Renal Replacement Therapy in patients with HIV/AIDS
Authors: Guo Hebing , Liu Jingyuan , Pu Lin , Hao Jingjing , Yin Ningning , Liu Yufeng , Xiong Haofeng , Li Ang 
Published: February 2020

Abstract
Background: Acute kidney injury (AKI) is a common complication among human immunodeficiency virus (HIV)-infected patients resulting in increased morbidity and mortality. Continuous renal replacement therapy (CRRT) is a useful method and instrument in critically ill patients with fluid overload and metabolic disarray, especially in those who are unable to tolerate the intermittent hemodialysis. However, the epidemiology, influence factors of CRRT and mortality in patients with HIV/AIDS are still unclear in China. This study aims to study the HIV-infected patients admitted in Intensive Care Unit (ICU) and explore the influence factors correlated with CRRT and their prognosis. Methods: We performed a retrospective case-control study in the ICU of the Beijing Ditan Hospital Capital Medical University. From June 1, 2005 to May 31, 2017, 225 cases were enrolled in this clinical study. Results: 122 (54.2%) patients were diagnosed with AKI during their stay in ICU, the number and percentage of AKI stage 1, 2 and 3 were 38 (31.1%), 21(17.2%) and 63(51.7%), respectively. 26.2% of AKI patients received CRRT during the stay of ICU. 56.25% CRRT patients died in ICU. The 28-day mortality was 62.5%, and the 90-day mortality was 75%. By univariate logistics analysis, it showed that higher likelihood of diagnosis for respiratory failure (OR=7.333,95% CI 1.467-36.664, p=0.015), higher likelihood of diagnosis for septic shock (OR=1.005,95% CI 1.001-1.01, p=0.018), and higher likelihood to use vasoactive agents (OR=10.667,95% CI 1.743-65.271, p=0.001), longer mechanical ventilation duration (OR=1.011,95% CI 1.002-1.019, p=0.011), higher likelihood for diagnosis for PCP (OR=7.50,95% CI 1.288-43.687, p=0.025), higher SOFA score at ICU admission (OR=1.183,95% CI 1.012-1.383, p=0.035), longer duration of CRRT (OR=1.014,95% CI 1.001-1.028, p=0.034) contributed to a higher mortality at ICU. The Cox Analysis for the cumulative survival of AKI 3 patients between the CRRT and non-CRRT groups shows no significant differences (p =0.595).Conclusion: There is a high incidence of AKI in HIV-infected patients admitted in our ICU. Patients with severe AKI were more prone to be admitted for CRRT and have a consequent poor prognosis.
Peripheral Anterior Chamber Depth and Screening Techniques for Primary Angle Closure Disease in Community Elderly Chinese
Authors: Xue Wenwen , Luo Qin , Yuan Yin , Fu Chaowei , He Jiangnan , Zou Haidong , Tong Xiaowei , Lee Richard K. , Ge Ling 
Published: February 2020

Abstract
Purpose

To investigate the distribution of peripheral anterior chamber depth (ACD) and the relationship between peripheral ACD and gonioscopy compared to other ocular parameters (peripheral ACD, spherical equivalent [SE], intraocular pressure [IOP]) for primary angle closure disease (PACD) screening in an elderly Chinese community.

Methods

Cross-sectional study. Adults 50 and older were enrolled from a population-based study using cluster random sampling in Pudong New District, Shanghai. Remote ocular screening was performed with digital anterior eye structure photography. Van Herrick measurements were used to evaluate the peripheral ACD, the depth of the peripheral anterior chamber, and corneal thickness (CT), and the ACD to CT ratio was calculated. Subjects with peripheral ACD less than 0.5 CT were made follow-up appointments for clinical examination with gonioscopy. Receiver operating characteristic curves (ROC) were generated to show the performance of different tests in screening for primary angle closure disease (PACD).

Results

2528 adults participated in the study with 91 patients diagnosed with PACD. 2463 subjects had valid data in the right eye available for analysis. The mean peripheral ACD values for men and women were significantly different: 1.04±0.46 (range 0.11-2.93) CT and 0.87±0.41 (range 0.12-2.96) CT respectively (t=-4.18; P<0.0001). Multivariate logistic regression analysis reveals that peripheral ACD declined by 0.31 CT (P<0.0001) per diopter of SE and was 0.19 CT (P<0.0001) shallower in women than in men (r2=0.1304, P<0.0001). Peripheral ACD performed best in screening for PACD.

Conclusions

Peripheral ACD measurement is recommended for PACD screening in community elderly Chinese.
Functionally enhanced placenta-derived mesenchymal stem cells inhibit adipogenesis in orbital fibroblast with graves’ ophthalmopathy
Authors: Kim Jae Yeon , Park Sohae , Lee Hyun-Jung , Lew Helen , Kim Gi Jin 
Published: February 2020

Abstract
Background: Placenta-derived mesenchymal stem cells (PD-MSCs) have unique immunomodulatory properties, and Phosphatase of regenerating liver-1 (PRL-1) regulates self-renewal ability of stem cells and promotes proliferation. Graves’ ophthalmopathy (GO) is an autoimmune inflammatory disease of the orbit and is characterized by increased orbital contents involving adipose tissue. Because the mechanism of inhibiting adipogenesis in orbital fibroblast (OF) with GO patients remains uncertain, the major objective is to investigate mechanisms alleviating adipogenesis by PRL-1 overexpressing PD-MSCs (PD-MSCsPRL-1, PRL-1+) in OF derived from GO patients.

Methods: primary OFs from patients with GO were isolated from orbital adipose tissue specimens. After maturation as adipogenic differentiation, normal and GO-derived OF were cocultured with naïve and PD-MSCsPRL-1. Western blotting were conducted for evaluating molecular mechanisms for inhibiting adipogenesis in GO.

Results: The characterizations of PD-MSCsPRL-1 were similar to naïve. OF with GO patients stimulated adipocyte differentiation were significantly decreased lipid accumulation by cocultivation with PD-MSCsPRL-1 comparing with naïve. The mRNA and protein expression of adipogenic markers was declined in PD-MSCsPRL-1. The expression of pPI3K/AKT/mTOR protein in OF with GO patients was downregulated by cocultivation with PD-MSCsPRL-1 secreted IGFBPs. Interestingly, IGFBP2, 4, and 7 expressions through integrin alpha 4 (ITGA4) and beta 7 (ITGB7) in PD-MSCsPRL-1 were higher than naïve and upregulated pFAK downstream factor.

Conclusion: Taken together, secreted IGFBPs by PD-MSCsPRL-1 via upregulating FAK and blocking IGF inhibit adipogenesis of OF with GO patients, providing novel therapeutic approach using functionally enhanced MSCs potential for degenerative diseases.
LncRNA SNHG5 promotes the proliferation and cancer stem cell-like properties of hepatocellular carcinoma by regulating UPF1 and Wnt signaling pathway
Authors: Li Yarui , Hu Junbi , Lu Guifang , Ren Mudan , Zhang Zhiyong , Guo Dan , Lu Xinlan , He Shuixiang 
Published: February 2020

Abstract
Background: It have been revealed that lncRNAs had a critical role in human cancers, including HCC. Our previous study found a lncRNA SNHG5 related to the development of HCC. However, the role and exact mechanism of SNHG5 in HCC proliferation and the liver CSC-like properties is still unclear. Materials and Methods: We investigated the HCC cells proliferation and CSC-like properties in vitro and in vivo by knockdown or upregulate SNHG5, and detected underlying mechanisms by qRT-PCR, Western blotting, bioinformatic analysis, luciferase reporter assay, RNA immunoprecipitation. Results: Knockdown of SNHG5 repressed HCC cells proliferation and CSC-like properties, while overexpression of SNHG5 promoted cells growth. At the same time, CSCs markers ( CD44, CD133 and ALDH1) and related transcription factors (OCT4, SOX2 and NANOG) were downregulated when SNHG5 knockdown. Further investigations showed that SNHG5 regulated the proliferation and CSC-like properties of HCC by binding UPF1 and regulating Wnt/β-catenin pathway. Conclusions: SNHG5 plays critical role to promote HCC cells proliferation and cancer stem cell-like properties via UPF1 and Wnt/β-catenin pathway.
Prevalence and perceived importance of racial matching in the psychotherapeutic dyad: A national survey of addictions treatment clinical practices
Authors: Steinfeldt Jesse A. , Clay Shondra L. , Priester Paul E. 
Published: February 2020

Abstract
Background: Despite conflicting results in the literature concerning its efficacy in practice, racial matching has been identified as a component of culturally sensitive treatment. Methods: This study examined the perceived importance and prevalence of racial matching by surveying a national sample of substance abuse treatment centers from the Substance Abuse and Mental Health Services Administration (SAMHSA). Results: Using univariate statistical analysis, results for the prevalence of racial matching revealed that in 58% of the clinics, there was the potential to match a counselor with a racially similar client, while in 39% of the clinics, there was no potential to provide such a match. Among the agencies that displayed a potential for racial matching, 26% of the respondents indicated that they never racially matched clients and therapists, 71% reported that they sometimes practice racial matching, 15% indicated that they usually racially match, and only 7% purported to always racially match clients and therapists. Results for the perceived importance of racial matching revealed that in both situations where treatment centers had the potential for racial matching and did not have the potential for racial matching, supervisors reported that it was relatively important to provide culturally sensitive treatment but that it was not as important to match clients in substance abuse centers with racially/ethnically similar counselors. Conclusion: The topic of racial matching can be very complex and has shown variation amongst substance abuse centers; however, this study emphasizes the importance of providing culturally sensitive treatment and an appreciation of differences among members within each racial group. Keywords: culturally responsive counseling; cross-racial counseling; addictions treatment
Recurrence of low-risk vaginal embryonal rhabdomyosarcoma: A case report and literature review
Authors: Peng Hongfa , You Yan , Li Lei 
Published: February 2020

Abstract
Background Low-risk vaginal embryonal rhabdomyosarcoma (ERMS) requires no radiotherapy (RT) for local control. Case summary A 32-month-old girl presented with an exophytic vaginal botryoid mass, which was confirmed of be ERMS. She was given two courses of vincristine, topotecan, and cyclophosphamide (VAC) as neoadjuvant therapy, after which she underwent a hysteroscopy and conservative resection of the vaginal lesion with a negative margin. She was diagnosed with low-risk ERMS (stage I, subgroup A and Group I) and was discharged after another four courses of VAS. However, twenty-eight months after the last treatment, she presented with a giant mass protruding through the vaginal introitus, which was confirmed to be a recurrence of ERMS. Despite multiple rounds of therapy, the patient died 39 months after her diagnosis, at 5 years of age. Conclusion When making the decision to eliminate RT for low-risk vaginal ERMS patients, the risk of local recurrence should be considered and emphasized.
Overall survival in patients with metastatic pancreatic cancer after surgically primary tumor resected: a SEER-based nomogram analysis
Authors: Pan Maoen , Yang Yuanyuan , Wu Xiaoting , Huang Heguang 
Published: February 2020

Abstract
Background This study aimed to establish and validate a nomogram to predict overall survival in patients with metastatic pancreatic cancer (mPC) after surgically primary tumor resected. Methods All mPC patients who underwent primary tumor resection at SEER database between 2004 and 2016 were identified. We randomly assigned two-thirds of the patients to the training group and one third to the validation group. In the training group, the Kaplan–Meier survival analysis was used to analyze survival outcomes. A univariate and multivariate cox regression analysis was used to identify significant prognostic factors for establishing a nomogram. The predictive accuracy and discriminative ability were measured by the concordance index (C-index) and risk group stratification. Results A total of 742 patients were included for analysis. Four significant prognostic factors were obtained and included in the nomogram. The nomogram showed an acceptable discrimination ability (C- index:0.711) and good calibration and was further validated in the validation cohort (C- index: 0.727). The nomogram total points (NTP) had the potential to stratify patients into 2-risk groups with a median OS of 11 and 4.5 months (P < 0.001), respectively. Conclusions The nomogram can provide considerable accuracy individual prediction OS outcomes in patients with metastatic pancreatic cancer undergone primary tumor surgery and it can guide clinicians to make decisions in the clinical therapies.