Expression-Based Cell Lineage Analysis inDrosophilaThrough a Course-Based Research Experience for Early Undergraduates
Authors: Olson John M , Evans Cory J , Ngo Kathy T , Kim Hee Jong , Nguyen Joseph Duy , Gurley Kayla G H , Ta Truc , Patel Vijay , Han Lisa , Truong-N Khoa T , Liang Letty , Chu Maggie K , Lam Hiu , Ahn Hannah G , Banerjee Abhik Kumar , Choi In Young , Kelley Ross G , Moridzadeh Naseem , Khan Awais M , Khan Omair , Lee Szuyao , Johnson Elizabeth B , Tigranyan Annie , Wang Jay , Gandhi Anand D , Padhiar Manish M , Calvopina Joseph Hargan , Sumra Kirandeep , Ou Kristy , Wu Jessie C , Dickan Joseph N , Ahmadi Sabrena M , Allen Donald N , Mai Van Thanh , Ansari Saif , Yeh George , Yoon Earl , Gon Kimberly , Yu John Y , He Johnny , Zaretsky Jesse M , Lee Noemi E , Kuoy Edward , Patananan Alexander N , Sitz Daniel , Tran PhuongThao , Do Minh-Tu , Akhave Samira J , Alvarez Silverio D , Asem Bobby , Asem Neda , Azarian Nicole A , Babaesfahani Arezou , Bahrami Ahmad , Bhamra Manjeet , Bhargava Ragini , Bhatia Rakesh , Bhatia Subir , Bumacod Nicholas , Caine Jonathan J , Caldwell Thomas A , Calica Nicole A , Calonico Elise M , Chan Carman , Chan Helen H-L , Chang Albert , Chang Chiaen , Chang Daniel , Chang Jennifer S , Charania Nauman , Chen Jasmine Y , Chen Kevin , Chen Lu , Chen Yuyu , Cheung Derek J , Cheung Jesse J , Chew Jessica J , Chew Nicole B , Chien Cheng-An Tony , Chin Alana M , Chin Chee Jia , Cho Youngho , Chou Man Ting , Chow Ke-Huan K , Chu Carolyn , Chu Derrick M , Chu Virginia , Chuang Katherine , Chugh Arunit Singh , Cubberly Mark R , Daniel Michael Guillermo , Datta Sangita , Dhaliwal Raj , Dinh Jenny , Dixit Dhaval , Dowling Emmylou , Feng Melinda , From Christopher M , Furukawa Daisuke , Gaddipati Himaja , Gevorgyan Lilit , Ghaznavi Zunera , Ghosh Tulika , Gill Jaskaran , Groves David J , Gurara Kalkidan K , Haghighi Ali R , Havard Alexandra L , Heyrani Nasser , Hioe Tanya , Hong Kirim , Houman Justin J , Howland Molly , Hsia Elaine L , Hsueh Justin , Hu Stacy , Huang Andrew J , Huynh Jasmine C , Huynh Jenny , Iwuchukwu Chris , Jang Michael J , Jiang An An , Kahlon Simran , Kao Pei-Yun , Kaur Manpreet , Keehn Matthew G , Kim Elizabeth J , Kim Hannah , Kim Michelle J , Kim Shawn J , Kitich Aleksandar , Kornberg Ross A , Kouzelos Nicholas G , Kuon Jane , Lau Bryan , Lau Roger K , Law Rona , Le Huy D , Le Rachael , Lee Carrou , Lee Christina , Lee Grace E , Lee Kenny , Lee Michelle J , Lee Regina V , Lee Sean H K , Lee Sung Kyu , Lee Sung-Ling D , Lee Yong Jun , Leong Megan J , Li David M , Li Hao , Liang Xingfu , Lin Eric , Lin Michelle M , Lin Peter , Lin Tiffany , Lu Stacey , Luong Serena S , Ma Jessica S , Ma Li , Maghen Justin N , Mallam Sravya , Mann Shivtaj , Melehani Jason H , Miller Ryan C , Mittal Nitish , Moazez Carmel M , Moon Susie , Moridzadeh Rameen , Ngo Kaley , Nguyen Hanh H , Nguyen Kambria , Nguyen Thien H , Nieh Angela W , Niu Isabella , Oh Seo-Kyung , Ong Jessica R , Oyama Randi K , Park Joseph , Park Yaelim A , Passmore Kimberly A , Patel Ami , Patel Amy A , Patel Dhruv , Patel Tirth , Peterson Katherine E , Pham An Huynh , Pham Steven V , Phuphanich Melissa E , Poria Neil D , Pourzia Alexandra , Ragland Victoria , Ranat Riki D , Rice Cameron M , Roh David , Rojhani Solomon , Sadri Lili , Saguros Agafe , Saifee Zainab , Sandhu Manjot , Scruggs Brooke , Scully Lisa M , Shih Vanessa , Shin Brian A , Sholklapper Tamir , Singh Harnek , Singh Sumedha , Snyder Sondra L , Sobotka Katelyn F , Song Sae Ho , Sukumar Siddharth , Sullivan Halley C , Sy Mark , Tan Hande , Taylor Sara K , Thaker Shivani K , Thakore Tulsi , Tong Gregory E , Tran Jacinda N , Tran Jonathan , Tran Tuan D , Tran Vivi , Trang Cindy L , Trinh Hung G , Trinh Peter , Tseng Han-Ching H , Uotani Ted T , Uraizee Akram V , Vu Kent K T , Vu Kevin K T , Wadhwani Komal , Walia Paluk K , Wang Rebecca S , Wang Shuo , Wang Stephanie J , Wiredja Danica D , Wong Andrew L , Wu Daniel , Xue Xi , Yanez Griselda , Yang Yung-Hsuan , Ye Zhong , Yee Victor W , Yeh Cynthia , Zhao Yue , Zheng Xin , Ziegenbalg Anke , Alkali Jon , Azizkhanian Ida , Bhakta Akash , Berry Luke , Castillo Ryen , Darwish Sonja , Dickinson Holly , Dutta Ritika , Ghosh Rahul Kumar , Guerin Riley , Hofman Jonathan , Iwamoto Garrick , Kang Sarah , Kim Andrew , Kim Brian , Kim Hanwool , Kim Kristine , Kim Suji , Ko Julie , Koenig Michael , LaRiviere Alejandro , Lee Clifton , Lee Jiwon , Lung Brandon , Mittelman Max , Murata Mark , Park Yujin , Rothberg Daniel , Sprung-Keyser Ben , Thaker Kunal , Yip Vivian , Picard Paul , Diep Francie , Villarasa Nikki , Hartenstein Volker , Shapiro Casey , Levis-Fitzgerald Marc , Jaworski Leslie , Loppato David , Clark Ira E , Banerjee Utpal 
Published: November 2019

AbstractA variety of genetic techniques have been devised to determine cell lineage relationships during tissue development. Some of these systems monitor cell lineages spatially and/or temporally without regard to gene expression by the cells, whereas others correlate gene expression with the lineage under study. The GAL4 Technique for Real-time and Clonal Expression (G-TRACE) system allows for rapid, fluorescent protein-based visualization of both current and past GAL4 expression patterns and is therefore amenable to genome-wide expression-based lineage screens. Here we describe the results from such a screen, performed by undergraduate students of the University of California, Los Angeles (UCLA) Undergraduate Research Consortium for Functional Genomics (URCFG) and high school summer scholars as part of a discovery-based education program. The results of the screen, which reveal novel expression-based lineage patterns within the brain, the imaginal disc epithelia, and the hematopoietic lymph gland, have been compiled into the G-TRACE Expression Database (GED), an online resource for use by the Drosophila research community. The impact of this discovery-based research experience on student learning gains was assessed independently and shown to be greater than that of similar programs conducted elsewhere. Furthermore, students participating in the URCFG showed considerably higher STEM retention rates than UCLA STEM students that did not participate in the URCFG, as well as STEM students nationwide.