bluuebunny/crossref_metadata_2025_split huggingface dataset:
MYC Targeted Rad50 Drives Progression of High-Grade Serous Ovarian Cancer via NF-κB Activation
Authors: Li Yinuo , Wang Shourong , Li Peng , Li Yingwei , Liu Yao , Fang Haiya , Zhang Xiyu , Liu Zhaojian , Kong Beihua 
Published: November 2020

Abstract
BackgroundRad50 is a component of MRN complex, which consists of Mre11-Rad50-Nbs1. The MRN complex participates in DNA double-strand break repair and DNA-damage checkpoint activation. We sought to investigate the clinical and functional significance of Rad50 in high-grade serous ovarian cancer. MethodsChromatin immunoprecipitation and luciferase assays were performed to evaluate the regulatory roles of MYC on Rad50 expression. Association between Rad50 expression and clinical outcome in HGSOCs was evaluated by Kaplan-Meier analysis. Invasion, clonogenic assay and xenograft mice model were conducted to determine to functional role of Rad50 in ovarian cancer. Protein immunoprecipitation and immunofluorescence were used to explore the underlying mechanisms. ResultsMYC proto-oncogene transcriptionally activated Rad50 expression in high-grade serous ovarian cancer. Next, we provided evidences that Rad50 was frequently upregulated in HGSOCs and enhanced Rad50 expression inversely correlated with patient’s survival. In addition, ectopic expression of Rad50 promoted proliferation/invasion and induced EMT of ovarian cancer cells, whereas knockdown of Rad50 led to decreased aggressive behaviors. Mechanistic investigations revealed that Rad50 induced aggressiveness in HGSOC via activation NF-κB signaling pathway. Moreover, we identified CARD9 as an interacting protein of Rad50 in ovarian cancer cells and activation of NF-κB pathway by Rad50 is CARD9 dependent. ConclusionsOur findings provide evidence that MYC targeted Rad50 exhibits oncogenic property via NF-κB activation in high-grade serous ovarian cancer.
Anaphylaxis following the consumption of pumpkin seeds in a two-year-old child tolerant to its pulp – a case study and a minireview
Authors: Gawryjołek Julia , Ludwig Hanna , Żbikowska-Gotz Magdalena , Bartuzi Zbigniew , Krogulska Aneta 
Published: November 2020

Abstract
BackgroundPumpkin and its seeds are increasingly consumed by children for their potential health benefits. Each day, around 30% of teenagers consume nuts and seeds, including pumpkin seeds. However, there is some evidence that pumpkin seeds may exert allergenic effects and induce severe life-threatening anaphylaxis. Allergy for melon, cucumber, and zucchini, which belong to the same Cucurbitaceae family as pumpkin, are well known, opposite to pumpkin allergy. Few descriptions of allergic reactions associated with pumpkin have been published, especially in children. To date, three cases of pumpkin pulp and two seed cases have been reported among children. Our case report describes a case of pumpkin seed anaphylaxis in a child with good tolerance of pulp.Case presentationIn the present study, a two-year-old child experienced anaphylaxis: generalized urticaria, swollen lips and increasing dyspnea following the consumption of pumpkin seeds. The history showed that although the child had been receiving elimination diet because of atopic dermatitis, the basic dietary element was pumpkin pulp. The skin-prick test was positive for pumpkin seeds (5 mm) but negative for pumpkin pulp (1 mm); allergen-specific IgE was 1.34 kUA/L for seed, and 0.37 kUA/L for pulp. The component resolved diagnosis found IgE to sesame seed, buckwheat and walnut (storage proteins). Based on these findings, severe anaphylactic reaction to pumpkin seeds was identified.ConclusionsIt is possible to develop pumpkin seed anaphylaxis in a child with a tolerance to pumpkin pulp. Pumpkin seeds allergens have not been well characterized. Homology between the amino acid sequences in storage proteins may indicate cross reactivity between different edible seeds and nuts.
A Framework of Evidence-Based Decision-Making in Health System Management: A Best-Fit Framework Synthesis and a Scoping Review
Authors: Shafaghat Tahereh , Bastani Peivand , Nasab Mohammad Hasan Imani , Bahrami Mohammad Amin , Kavosi Zahra , Montazer Mahsa Roozrokh Arshadi , Zarchi Mohammad Kazem Rahimi , Edirippulige Sisira 
Published: November 2020

Abstract
Background: Scientific evidence is the basis for improving public health; decision-making without sufficient attention to evidence may lead to unpleasant consequences. Despite efforts to create comprehensive guidelines and models for evidence-based decision-making (EBDM), there isn`t any to make the best decisions concerning scarce resources and unlimited needs. The present study aimed to develop a comprehensive applied framework for EBDM. Methods: This was a meta-synthesis including two phases of a Scoping Review (SR) and a Best-Fit Framework (BFF) synthesis conducted in 2019. A scoping review was done for the comprehensive review of the existing published studies in this area. The six-stage approach of Arksey and O’Malley was applied. Six main databases including PUBMED, Scopus, Web of Science, Science Direct, EMBASE, and ProQuest were searched using related keywords. Data were extracted and analyzed via thematic analysis. Results of the scoping review were then synthesized to achieve the best-fit framework applying Carroll et al (2013) approach. Results: Based on the SR, 3751 studies were found, and due to the full-text screening of the studies, 30 final articles were selected for extracting the components and steps of EBDM in Health System Management (HSM). After collecting, synthesizing, and categorizing key information, the framework of EBDM in HSM was developed in the form of four general scopes. These comprised inquiring, inspecting, implementing, and integrating, which included 10 main steps and 56 sub-steps. Conclusions: The present framework provided a comprehensive guideline that can be well adapted for implementing EBDM in health systems and related organizations especially in underdeveloped and developing countries where there is usually a lag in updating and applying evidence in their decision-making process. In addition, this framework by providing a complete, well-detailed, and sequential process can be tested in the organizational decision-making process by developed countries to improve their EBDM cycle.
Functional and radiological outcomes after treatment with custom-made acetabular components in patients with Paprosky type 3 acetabular defects: short-term results
Authors: Gruber Michael Stephan , Jesenko Michael , Burghuber Julia , Hochreiter Josef , Ritschl Peter , Ortmaier Reinhold 
Published: November 2020

Abstract
Background Severe acetabular defects require special treatment with either impaction bone grafting, metal augmented cups or cup-cage constructs. Even these options are often not adequate, especially in hips with Paprosky type 3 defects with loss of anterior and posterior columns. This study investigates the clinical and radiological outcomes of custom-made acetabular components (© Materialise NV, Leuven, Belgium) for Paprosky type 3 defects.Methods Sixteen patients were eligible for this trial, nine of whom agreed to be included. All of them completed one year of follow-up. The Harris hip score and the Oxford hip score were used to compare pre- and postoperative functional outcomes. Radiological follow-up comprised anteversion and inclination of the implanted cup and offset measurements in both hips (femoral, medial, ischial offset and center of rotation). Statistical analyses were performed with IBM SPSS Statistics.Results The mean follow-up time of the nine patients was 12.2 months (range: 10 - 18). The Oxford hip score and Harris hip score improved from 19.8 and 50.1 to 29.4 and 68.8, respectively (p = 0.009 and 0.01). There were complications in three cases (33.3%), which led to one re-revision (11.1%). Radiologic follow-up showed restoration of the height of the center of rotation and of the global offset. Significant difference was detected in the femoral offset.Conclusions The functional and radiological outcomes are promising. However, long-term outcomes still need to be examined.Level of Evidence: Therapeutic Level IV.
Accelerated fetal growth in early pregnancy and risk of preterm birth: a prospective cohort study
Authors: Elenis Evangelia , Wikström Anna-Karin , Simic Marija 
Published: November 2020

Abstract
Background: Preterm birth (occurring before 37 completed weeks of gestation) affects 15 million infants annually, 7.5% of which die due to related complications. The detection and early diagnosis are therefore paramount in order to prevent the development of prematurity and its consequences. So far, focus has been laid on the association between reduced intrauterine fetal growth during late gestation and prematurity. The aim of the current study was to investigate the association between accelerated fetal growth in early pregnancy and the risk of preterm birth. Methods: This prospective cohort study included 69 617 singleton pregnancies without congenital malformations and with available biometric measurements during the first and second trimester. Estimation of fetal growth was based on measurements of biparietal diameter (BPD) at first and second trimester scan. We investigated the association between accelerated fetal growth and preterm birth prior to 37 weeks of gestation. The outcome was further stratified into very preterm birth (before 32 weeks of gestation) or moderate preterm birth (between 32 and 37 weeks of gestation) and medically induced or spontaneous preterm birth and was further explored. Results: The odds of prematurity were increased among fetuses with accelerated BPD growth (> 90th centile) estimated between first and second ultrasound scan, even after adjustment for possible confounders (aOR 1.36; 95% CI 1.20-1.54). The findings remained significant what regards moderate preterm births but not very preterm births. Regarding medically induced preterm birth, the odds were found to be elevated in the group of fetuses with accelerated growth in early pregnancy (aOR 1.34; 95% CI 1.11-1.63). On the contrary, fetuses with delayed fetal growth exhibited lower odds for both overall and spontaneous preterm birth.Conclusions: Fetuses with accelerated BPD growth in early pregnancy, detected by ultrasound examination during the second trimester, exhibited increased odds of being born preterm. The findings of the current study suggest that fetal growth in early pregnancy should be taken into account when assessing the risk for preterm birth.
Life Cycle Assessment of opencast lignite mining
Authors: Lelek Lukasz , Kulczycka Joanna 
Published: November 2020

Abstract
The life cycle phase of fossil fuel extraction is mainly considered in the LCA when evaluating the energy production processes. It is then only one of many unit processes, which contribute to the blurring of mining-relevant results. There are few items in the literature focusing exclusively on the lignite mining phase and analysing the specific mining conditions and associated environmental impacts. The article focuses on the LCA of lignite mining pro-cesses on the basis of data coming from a Polish mine. The technology for opencast lignite mining is noted for its high production efficiency, high level of recovery and lower risk as regards the safety of workers when compared with underground mining systems. However, the need to remove large amounts of overburden to uncover the deposit contributes to a much greater degradation of the landscape. Analysing the results obtained, several key (hot spot) elements of the lignite mining operations were distinguished for modelling the environmental impact, i.e.: calorific value, the amount of electricity consumption, the manner in which waste and overburden are managed. As a result there is a high sensitivity of the final indicator to changes in these impacts.
The Performance of Membrane Sweep in Conjunction with the Induction of Labor with Sublingual Misoprostol
Authors: Lella Mounica , Shetty Jyothi , Kyalakond Keerti , Pai Murlidhar V 
Published: November 2020

Objective:
To study the outcomes of membrane sweep done during induction of labor with sublingual misoprostol
at term pregnancy such as the mode of delivery and the induction delivery interval.

Methods:
This prospective descriptive study was conducted in 305 term women (237 primigravida and 68 multigravida)
who received sublingual misoprostol followed by membrane sweep (single or double sweep based on the progress of labor).
Main outcome measures included mode of delivery, induction to delivery interval, duration of labor, neonatal outcomes and
maternal complications.

Results:
Among 237 primigravida, 108 (45.5%) women delivered after a single sweep and 129 (54.4%) women needed a
second sweep. Among 68 multigravida, 47 (69.1%) delivered after a single sweep and only 21 (30.8%) required a second
sweep. Maximum number (75.5%) of primigravida had pre-induction Bishop score of 2 while maximum number (67.6%)
of multigravida had pre-induction Bishop score of 3. Vaginal deliveries (including instrumental delivery) were more in both
primigravida (55.6%) and multigravida (88.2%) who received membrane sweep in conjunction with induction of labor. The
mean time from the first dose of sublingual misoprostol to the onset of contraction was 6.9 hours in primigravida and 4.2
hours in multigravida while the meantime from the onset of contractions till vaginal delivery (duration of labor) was 11.2
hours in primigravida and 5.8 hours in multigravida. The mean interval from induction to the vaginal delivery was 18.8
hours in primigravida and 14.4 hours in multigravida.

Conclusion:
Sublingual misoprostol, along with membrane sweep at the initiation of labor induction is an effective
intervention despite its association with increased rate of cesarian section among primigravida as compared to the literature.
Deep Transfer Learning for COVID-19 Prediction: Case Study for Limited Data Problems
Authors: Albahli Saleh , Albattah Waleed 
Published: November 2020

Objective:
Automatic prediction of COVID-19 using deep convolution neural networks
based pre-trained transfer models and Chest X-ray images.

Methods:
This research employs the advantages of computer vision and medical image analysis to
develop an automated model that has the clinical potential for early detection of the disease. Using
Deep Learning models, the research aims at evaluating the effectiveness and accuracy of different
convolutional neural networks models in the automatic diagnosis of COVID-19 from X-ray images
as compared to diagnosis performed by experts in the medical community.

Result:
Due to the fact that the dataset available for COVID-19 is still limited, the best model to
use is the InceptionNetV3. Performance results show that the InceptionNetV3 model yielded the
highest accuracy of 98.63% (with data augmentation) and 98.90% (without data augmentation)
among the three models designed. However, as the dataset gets bigger, the Inception ResNetV2
and NASNetlarge will do a better job of classification. All the performed networks tend to over-fit
when data augmentation is not used, this is due to the small amount of data used for training and
validation.

Conclusion:
A deep transfer learning is proposed to detecting the COVID-19 automatically from
chest X-ray by training it with X-ray images gotten from both COVID-19 patients and people with
normal chest X-rays. The study is aimed at helping doctors in making decisions in their clinical
practice due its high performance and effectiveness, the study also gives an insight to how transfer
learning was used to automatically detect the COVID-19.
Design, Characterization and Clinical Evaluation of Curcumin Dental Film for the Treatment of Periodontitis
Authors: Samal Himansu B. , Patra Ch. Niranjan , Boyeena Lavanya , Sreenivas S. A. , Das Itishree Jogamaya 
Published: November 2020

Background::Periodontitis is one of the most widespread oral diseases. Curcumin, a hydrophobic polyphenolic compound derived from the rhizomes of Curcuma longa has a great potential for biological and pharmacological activities. The introduction of local controlled delivery of curcumin directly into the periodontal pocket is a promising approach for the treatment of periodontitis.Objective::The study has been designed to formulate intrapocket dental film loaded with curcumin to be used for insertion into the periodontal pockets and evaluate its clinical efficacy.Methods::Curcumin dental films were prepared by the solvent casting technique using polymers such as Ethylcellulose, HPMC K4M, Eudragit RL 100, Chitosan with Dibutyl Phthalate and Poly Ethylene Glycol 400 as the plasticizer. The prepared films (CDF1-CDF16) were evaluated for various physicochemical parameters employing established pharmaceutical procedures such as surface pH, folding endurance, tensile strength, swelling index, drug content, in-vitro drug release, antibacterial efficacy, stability studies, and clinical efficacy.Results::Experimental parameters of the obtained film exhibited results within the desired limit. Fourier Transform Infrared Spectroscopy and Differential Scanning Calorimetry studies revealed the formulated film to be stable during drug stability and compatible between drugs and excipients. The surface morphology of the formulation using Scanning Electron Microscope images showed the presence of free drug particles on the surface, causing a burst effect at the desired site. In-vitro dissolution studies showed an initial burst release to achieve an immediate therapeutic level of drug in the periodontal pocket followed by a progressive fall and extended-release of the drug for 10 days. The therapeutics effects, such as antibacterial activity of films on P. gingivalis, were also observed. Furthermore, the clinical trials of formulation CDF16 on affected patients proved the therapeutic efficacy of the dental films showing a statistically significant (p < 0.05) decrease in the clinical markers of periodontitis, i.e. periodontal probing depth, plaque index, and sulcular bleeding index.Conclusion::The experimental results suggest that Curcumin dental film can be experimentally identified as a potential drug delivery device. Hence, curcumin dental film can be used for topical treatment of periodontal diseases and to achieve optimum therapeutic efficacy.
Development of Antibacterial Xanthan/Chitosan Biguanidine Hydrochloride Polyelectrolyte Complexes Decorated with Eco-friendly Prepared Silver Nanoparticles
Authors: Salama Hend Ezzat , Abdel Aziz Mohamed Samir 
Published: November 2020

Background:
Novel eco-friendly silver nanocomposites of xanthan/chitosan biguanidine
hydrochloride polyelectrolyte complexes were successfully prepared.

Methods:
Silver nanoparticles (AgNPs) were formed through an insitu eco-friendly reduction by the
non-toxic polysaccharides without the usage of toxic reagents. FTIR confirmed the successful preparation
of the nanocomposites while XRD confirmed the presence of AgNPs with face-centered cubic
structures. TEM confirmed the homogeneous distribution of AgNPs with an average size of 14.1 nm.
SEM was used to study the surface morphology of the nanocomposites while the energy-dispersive
X-ray spectroscopy (EDX) confirmed the presence of AgNPs.

Results:
Thermogravimetric analysis showed that the thermal stability was improved in the presence
of AgNPs as detected from the calculated integral procedure decomposition temperature. Antibacterial
activity against different bacteria species was significantly improved upon increasing the content
of AgNPs.

Conclusion:
Due to their interesting properties, the prepared polyelectrolyte complexes and their
AgNPs nanocomposites could be employed potentially in many biomedical applications like drug delivery.
Fabrication and Mechanical Properties of POSS Coated CNTs Reinforced Expancel Foam Core Sandwich Structures
Authors: Jones Wanda , Sapkota Bedanga , Simpson Brian , Hassan Tarig A. , Jeelani Shaik , Rangari Vijaya 
Published: November 2020

Background:
Sandwich structures are progressively being used in various engineering
applications due to the superior bending-stiffness-to-weight ratio of these structures. We adapted a
novel technique to incorporate carbon nanotubes (CNTs) and polyhedral oligomeric silsesquioxanes
(POSS) into a sandwich composite structure utilizing a sonochemical and high temperature
vacuum assisted resin transfer molding technique.

Objective:
The objective of this work was to create a sandwich composite structure comprising of a
nanophased foam core and reinforced nanophased face sheets, and to examine the thermal and mechanical
properties of the structure. To prepare the sandwich structure, POSS nanoparticles were
sonochemically attached to CNTs and dispersed in a high temperature resin system to make the
face sheet materials and also coated on expandable thermoplastic microspheres for the fabrication
of foam core materials.

Methods:
The nanophased foam core was fabricated with POSS infused thermoplastic microspheres
(Expancel) using a Tetrahedron MTP-14 programmable compression molder. The reinforced
nanophased face sheet was fabricated by infusing POSS coated CNT in epoxy resin and
then curing into a compression stainless steel mold.

Result:
Thermal analysis of POSS-infused thermoplastic microspheres foam (TMF) showed an increase
in thermal stability in both nitrogen and oxygen atmospheres, 19% increase in thermal residue
were observed for 4 wt% GI-POSS TMF compared to neat TMF. Quasi-static compression results
indicated significant increases (73%) in compressive modulus, and an increase (5%) in compressive
strength for the 1 wt% EC-POSS/CNTs resin system. The nanophased sandwich structure
constructed from the above resin system and the foam core system displayed an increase (9%) in
modulus over the neat sandwich structure.

Conclusion:
The incorporation of POSS-nanofillier in the foam core and POSS-coated nanotubes in the face sheet significantly improved the thermal and mechanical properties of sandwich structure. Furthermore, the sandwich structure that was constructed from nanophased resin system showed an increase in modulus, with buckling in the foam core but no visible cracking.
5-Fluorouracil Loaded Orally Administered WGA-decorated Poly(lacticco- glycolic Acid) Nanoparticles for Treatment of Colorectal Cancer: In Vivo Evaluation
Authors: Pandey Aditya N. , Rajpoot Kuldeep , Jain Sunil K. 
Published: November 2020

Background::
Several studies have suggested the potential aptitude of polylactic-co-glycolic
acid (PLGA)-derived nanoparticles (NPs) to improve the antitumor efficacy of anticancer
drugs against colon cancer. Further, conjugation of lectins over the surface of the NPs may ameliorate
interaction and thus enhance the attachment of NPs with receptors.

Objective::
The main goal of the study was to prepare and evaluate the targeting potential (in vivo)
of the optimized NPs against colorectal cancer.

Methods::
The 5-fluorouracil (5-FU) loaded and wheat germ agglutinin (WGA)-conjugated PLGA-
NPs (WFUNPs) were prepared and then they were evaluated in vivo for targeting aptitude of
formulation using gamma scintigraphy after oral delivery. The WGA-conjugated and non-conjugated
optimized NPs were compared for any significant results. Further, optimized formulations were
also assessed for different parameters such as radiolabeling efficiency, sodium pertechnetate uptake,
stability of NPs, and organ distribution study.

Results::
Findings suggested prolonged retention of 99mTc-tagged WFUNPs in the colonic region after
24 h study. Eventually, the outcome from conjugated formulation revealed enhanced bioavailability
of the drug in blood plasma for up to 24 h.

Conclusion::
In conclusion, WGA-conjugation to NPs could improve the performance of the PLGA-
NPs in the treatment of colorectal cancer.