bluuebunny/crossref_metadata_2025_split huggingface dataset:
The haloes and environments of nearby galaxies (HERON) – II. The outer structure of edge-on galaxies
Authors: Mosenkov Aleksandr , Rich R Michael , Koch Andreas , Brosch Noah , Thilker David , Román Javier , Müller Oliver , Smirnov Anton , Usachev Pavel 
Published: March 2020

ABSTRACT
The haloes and environments of nearby galaxies (HERON) project is aimed at studying haloes and low surface brightness (LSB) details near galaxies. In this second HERON paper, we consider in detail deep imaging (down to surface brightness of ∼28 mag arcsec−2 in the r band) for 35 galaxies, viewed edge-on. We confirm a range of LSB features previously described in the literature but also report new ones. We classify the observed outer shapes of the galaxies into three main types (and their prototypes): disc/diamond-like (NGC 891), oval (NGC 4302), and boxy (NGC 3628). We show that the shape of the outer disc in galaxies does not often follow the general 3D model of an exponential disc: 17 galaxies in our sample exhibit oval or even boxy isophotes at the periphery. Also, we show that the less flattened the outer disc, the more oval or boxy its structure. Many galaxies in our sample have an asymmetric outer structure. We propose that the observed diversity of the galaxy outer shapes is defined by the merger history and its intensity: if no recent multiple minor or single major merging took place, the outer shape is diamond-like or discy. On the contrary, interacting galaxies show oval outer shapes, whereas recent merging appears to transform the outer shape to boxy.
Introduction to immunotherapy for brain tumor patients: challenges and future perspectives
Authors: Montoya Megan L , Kasahara Noriyuki , Okada Hideho 
Published: March 2020

AbstractMalignant gliomas, including glioblastoma (GBM) as the most aggressive type of adult CNS tumors, are notoriously resistant to current standard of care treatments, including surgery, systemic chemotherapy, and radiation therapy (RT). This lack of effective treatment options highlights the urgent need for novel therapies, including immunotherapies. The overarching goal of immunotherapy is to stimulate and activate the patient’s immune system in a targeted manner to kill tumor cells. The success of immunotherapeutic interventions in other cancer types has led to interest in and evaluation of various experimental immunotherapies in patients with malignant gliomas. However, these primary malignant brain tumors present a challenge because they exist in a vital and sensitive organ with a unique immune environment. The challenges and current status of experimental immunotherapeutic approaches, including vaccines, immune-checkpoint blockade, chimeric antigen receptor T-cell therapy, and oncolytic viruses will be discussed, as well as the potential for combinatorial therapies.
A Pilot Randomized Clinical Trial of Brief Interventions to Encourage Quit Attempts in Smokers From Socioeconomic Disadvantage
Authors: Steinberg Marc L , Rosen Rachel L , Versella Mark V , Borges Allison , Leyro Teresa M 
Published: March 2020

Abstract

Introduction
Cigarette smoking disproportionately affects communities of low socioeconomic status where greater smoking prevalence and poorer cessation rates have been observed. Utilizing brief evidence-based interventions to increase cessation attempts may be an effective and easily disseminable means by which to mitigate undue burden in this population.

Aims and Methods
The current intervention randomized daily smokers (N = 57) recruited from a local community soup kitchen to receive either Brief (eg, 30 m) Motivational Interviewing, Nicotine Replacement Therapy (NRT) sampling, or a Referral-Only intervention. Approximately half of participants (50.9%) reported not completing high school and many reported either just (41.4%) or not (40.4%) meeting basic expenses. Follow-up was completed approximately 1-month postintervention.

Results
Nonsignificant group differences indicated that participants randomized to the NRT sampling condition were more likely to make a quit attempt (moderate effect size). Approximately 40% of the sample reported making a serious quit attempt at follow-up. Significant differences in cigarettes per day at follow-up, controlling for baseline, were observed, with participants in the Motivational Interviewing condition, only, reporting significant reductions. Participants randomized to the NRT condition were significantly more likely to report using NRT patch and lozenge at follow-up (large effect). There were no differences between groups with respect to seeking behavioral support. Finally, we found that subjective financial strain moderated the effect of condition on change in cigarette consumption where NRT sampling was more effective for participants reporting less financial strain.

Conclusions
Findings provide initial evidence for personalizing brief interventions to promote quit attempts in low-income smokers.

Implications
While most clinical research on tobacco use and dependence focuses on successful sustained abstinence, the current study is novel because it examined three brief interventions designed to increase the number of quit attempts made by a nontreatment-seeking group suffering from health disparities (ie, smokers from socioeconomic disadvantage). These data suggest that nontreatment-seeking smokers from socioeconomic disadvantage can be influenced by Brief MIs and these interventions should be used to motivate smokers from socioeconomic disadvantage to make a quit attempt. Future studies should examine combined MIs including pharmacological and behavioral interventions.
Relapsing Uveitis due to Human T-lymphotropic Virus Type 1 in a Patient Living With HIV Diagnosed by Metagenomic Deep Sequencing
Authors: Phadke Varun K , Shantha Jessica G , O’Keefe Ghazala 
Published: March 2020

Abstract
HIV infection can result in vision loss from different causes, including HIV retinopathy and uveitis secondary to other infections, such as toxoplasmosis and viral retinitis. It is imperative to identify any infectious causes of uveitis to successfully treat the condition and prevent further vision loss. Metagenomic deep sequencing (MDS) is an emerging technology that presents an unbiased approach to the evaluation of clinical syndromes, including uveitis, that have not been diagnosed by pathogen-specific testing. Herein we present a case of a woman living with HIV with 11 years of relapsing bilateral uveitis refractory to systemic corticosteroid therapy who was diagnosed with human T-lymphotropic virus type 1 (HTLV-1)–associated uveitis by this technology. We also briefly review the literature of MDS as a diagnostic tool and the epidemiology, pathogenesis, and diagnosis of HTLV-1-associated uveitis.
Mitochondrial Pentatricopeptide Repeat Protein, EMB2794, Plays a Pivotal Role in NADH Dehydrogenase Subunit nad2 mRNA Maturation in Arabidopsis thaliana
Authors: Marchetti Fernanda , Cainzos Maximiliano , Shevtsov Sofía , Córdoba Juan Pablo , Sultan Laure Dora , Brennicke Axel , Takenaka Mizuki , Pagnussat Gabriela , Ostersetzer-Biran Oren , Zabaleta Eduardo 
Published: March 2020

AbstractThe Arabidopsis genome encodes &gt;450 proteins containing the pentatricopeptide repeat (PPR) motif. The PPR proteins are classified into two groups, termed as P and P Long-Short (PLS) classes. Typically, the PLS subclass proteins are mainly involved in the RNA editing of mitochondrial and chloroplast transcripts, whereas most of the analyzed P subclass proteins have been mainly implicated in RNA metabolism, such as 5′ or 3′ transcript stabilization and processing, splicing and translation. Mutations of PPR genes often result in embryogenesis and altered seedling developmental defect phenotypes, but only a limited number of ppr mutants have been characterized in detail. In this report, we show that null mutations in the EMB2794 gene result in embryo arrest, due to altered splicing of nad2 transcripts in the Arabidopsis mitochondria. In angiosperms, nad2 has five exons that are transcribed individually from two mitochondrial DNA regions. Biochemical and in vivo analyses further indicate that recombinant or transgenic EMB2794 proteins bind to the nad2 pre-mRNAs in vitro as well as in vivo, suggesting a role for this protein in trans-splicing of nad2 intron 2 and possibly in the stability of the second pre-mRNA of nad2. Homozygous emb2794 lines, showing embryo-defective phenotypes, can be partially rescued by the addition of sucrose to the growth medium. Mitochondria of rescued homozygous mutant plants contain only traces of respiratory complex I, which lack the NADH-dehydrogenase activity.
Feeding and growth efficiency in a pelagic chaetognath, Zonosagitta nagae reared in the laboratory
Authors: Otake Shiori , Shimode Shinji , Takahashi Kazutaka 
Published: March 2020

AbstractChaetognaths are ubiquitous in the marine environment and are among the most abundant carnivorous plankton. Nevertheless, characterization of physiological parameters remains limited largely due to the difficulty in obtaining the data from pelagic chaetognaths in laboratory studies. This study therefore aimed to determine the feeding and growth rates of Zonosagitta nagae under laboratory rearing condition. A total of 54 field-collected chaetognaths with maturity Stage I and II ranging from 5 to 12 mm in body length collected in June, August and October were individually incubated at 20–22°C with excess copepod prey and growth parameters were obtained from 26 individuals that survived ~10–46 days. For Z. nagae, ingestion and growth rates increased with body dry weight, and both parameters were significantly related, indicating that gross growth efficiency was ~47%. Specific ingestion rates ranged from 0.03 to 0.43 d−1 and tended to decrease with an increase of size of the chaetognaths. Individual specific growth rate varied widely in small-sized individuals (~6 mm), ranging from −0.103 to 0.135, but became relatively constant (~0.032) with increasing size. Overall the study results suggest that Z. nagae is characterized by high gross growth efficiency, which is in striking contrast with previously studied inshore species.
Can Patient Expectations of Returning to Work Documented Before, During, or at the End of Treatment Predict Actual Return to Work Post-treatment? An Evidence-Based Structured Systematic Review
Authors: Fishbain David A , Pulikal Aditya 
Published: March 2020

Abstract

Background
There is significant evidence that patient expectations can impact their treatment outcomes. The hypothesis of this systematic review was then the following. There will be significant consistent evidence as specified by the Agency for Health Care Policy and Research (AHCPR) guidelines that chronic pain patient (CPP) expectations for returning to work documented before, during, or at the end of treatment will predict actual return to work post-treatment.

Methods
Of 316 references, 12 studies fulfilled inclusion criteria. These studies analyzed expectations of returning to work documented before, during, or at the end of treatment and utilized these for predicting return to work post-treatment. Relevant aspects of these studies were abstracted into tabular form for numerical analysis. All studies were rated independently by two reviewers for quality. The percentage of the 12 studies supporting the hypothesis was determined. This was then utilized to determine an AHCPR guideline rating for consistency.

Results
No studies had a rejection quality score. All studies were type 4. The hypothesis was supported by 91.6% of the studies. According to the AHCPR guidelines, this translated into an A rating: consistent findings from multiple type 4 studies.

Conclusions
CPP expectations of returning to work as documented before, during, or at the end of treatment may predict actual return to work post-treatment.
A systematic review exploring the evidence reported to underpin exercise dose in clinical trials of rheumatoid arthritis
Authors: Boniface Graham , Gandhi Varsha , Norris Meriel , Williamson Esther , Kirtley Shona , O’Connell Neil E 
Published: March 2020

Abstract
We aimed to evaluate the evidence reported to underpin exercise dose in randomised controlled trials (RCTs) using strengthening exercise in RA. We searched six different databases between 1 January 2000 and 3 April 2019. We included RCTs, where a main component of the intervention and/or control used strengthening exercise. Evidence sources cited to underpin dose were judged for their quality, consistency and applicability. Thirty-two RCTs were reviewed. Four (12.5%) piloted the intervention without using dose-escalation designs to determine optimal dose-response. Twenty (62.5%) reported no evidence underpinning dose. Where reported, quality, consistency and applicability of the underpinning evidence was a cause for methodological concern. The majority of RCTs did not report the evidence underpinning dose. When reported, the evidence was often not applicable to the clinical population. Frequently, the dose used differed to the dose reported/recommended by the underpinning evidence. Our findings illustrate exercise dose may not be optimised for use with clinical populations prior to evaluation by RCT.
DOES LOSS OF 131I COUNTS NEED CORRECTION IN GAMMA-CAMERA IMAGING IN CHILDREN AND YOUNG ADULTS IN POST-THERAPEUTIC SCANS? COMPARISON OF PHANTOM VERSUS PATIENT STUDY
Authors: Kumar Praveen , Bal Chandrasekhar , Damle Nishikant A , Dwivedi S N , Agarwala Sandeep 
Published: March 2020

Abstract
This study aimed to verify whether there is whole-body (WB) count loss due to dead time of gamma camera when high amount of 131I is administered to patients. Planar views of a phantom containing 5751 MBq of 131I were acquired at 24-h intervals for 68 d. Eighty-two patients ≤21 y old were given diagnostic activity (74 MBq) followed by therapeutic activity (1110–5772 MBq). WB scans of patients were acquired at 2 h after diagnostic and therapeutic activity administration. Count loss in patients and phantom were compared. In phantom, there was no count loss up to 139 MBq. At maximum activity of 5751 MBq 131I, the loss was 46%. In patients, the average WB count loss was insignificant after the administration of therapeutic activity. Count loss due to dead time in phantom differed significantly from patient results that can probably be explained by the distribution of activity over a large area in vivo.
Modeling multipartite virus evolution: the genome formula facilitates rapid adaptation to heterogeneous environments†
Authors: Zwart Mark P , Elena Santiago F 
Published: March 2020

Abstract
Multipartite viruses have two or more genome segments, and package different segments into different particle types. Although multipartition is thought to have a cost for virus transmission, its benefits are not clear. Recent experimental work has shown that the equilibrium frequency of viral genome segments, the setpoint genome formula (SGF), can be unbalanced and host-species dependent. These observations have reinvigorated the hypothesis that changes in genome-segment frequencies can lead to changes in virus-gene expression that might be adaptive. Here we explore this hypothesis by developing models of bipartite virus infection, leading to a threefold contribution. First, we show that the SGF depends on the cellular multiplicity of infection (MOI), when the requirements for infection clash with optimizing the SGF for virus-particle yield per cell. Second, we find that convergence on the SGF is very rapid, often occurring within a few cellular rounds of infection. Low and intermediate MOIs lead to faster convergence on the SGF. For low MOIs, this effect occurs because of the requirements for infection, whereas for intermediate MOIs this effect is also due to the high levels of variation generated in the genome formula (GF). Third, we explored the conditions under which a bipartite virus could outcompete a monopartite one. As the heterogeneity between environments and specificity of gene-expression requirements for each environment increased, the bipartite virus was more likely to outcompete the monopartite virus. Under some conditions, changes in the GF helped to exclude the monopartite competitor, highlighting the versatility of the GF. Our results show the inextricable relationship between MOI and the SGF, and suggest that under some conditions, the cost of multipartition can be outweighed by its benefits for the rapid tuning of viral gene expression.
Niche divergence corresponds to genetic differentiation within the parrot-beaked tortoiseHomopus areolatus(Reptilia: Testudinidae), endemic to South Africa
Authors: Hofmeyr Margaretha D , Ihlow Flora , Fouche Pierre , Daniels Savel R 
Published: March 2020

AbstractWe assessed genetic differentiation and habitat suitability for Homopus areolatus during current and Last Glacial Maximum (LGM) conditions. The ND4 locus retrieved two monophyletic mtDNA clades with lower diversity in clade 1, in the west, than in clade 2, in the south-east. Clade 1 showed a north–south and clade 2 a west-to-east genetic divergence, and the clades co-occur in the syntaxis zone of the Cape Fold Mountains. The clades occupy distinct niches with limited overlap. Rainfall seasonality contributed most to habitat suitability, with clade 1 being restricted to winter rainfall and clade 2 to all-year rainfall regions. Precipitation variables contributed 90% and 60%, respectively, to habitat suitability of clades 1 and 2, with temperature, particularly mean temperature of the driest quarter, being of greater importance for clade 2 than clade 1. Suitable habitat shrank from the LGM to current conditions, probably due to reduced rainfall in the west and higher temperatures in most regions. We conclude that patterns of genetic divergence are strongly associated with ecological niche divergence of H. areolatus clades. More studies are needed to assess the taxonomic status of clade 1, particularly in view of its shrinking habitat due to climate change and anthropogenic factors.
Sex Bias and Genotype Influence on Opioid Safety Profile in Chronic Low Back Pain
Authors: Margarit César , Roca Reyes , Inda María-del-Mar , Muriel Javier , Ballester Pura , Flor Andrea , Morales Domingo , Peiró Ana M. 
Published: March 2020

Objectives:The use of opioids to relieve pain is a challenge because of the high variability in dose requirements and tolerance profiles. Among potential modulators are the individual’s genetic background and being female. Our aim was to evaluate sex bias and genotype-related influence on opioid titration safety, in chronic low back pain (CLBP), the most frequent chronic noncancer pain.Methods:A 3-year prospective study was developed in opioid-naive CLBP patients. Data were self-reported by patients (pain [Visual Analogy Scale], adverse events [AEs], and health care resource utilization) and physicians (analgesic prescription, morphine equivalent daily dose, and suspected adverse drug reactions [ADRs]). Outcomes were analyzed as patients with AEs (case) or without (control) together with patients’ sex and genotype. Gene variants inOPRM1(rs1799971),COMT(rs4680),ABCB1(rs1045642),UGT2B7(rs12233719 and rs7438135),KCNJ6(rs2070995 and rs6517442), andCYP3A5*3(rs776746) were assessed. The hospital ethics committee approved the study, and statistical analyses were performed with R, v.3.2.4.Results:A total of 179 patients were included (64% female, mean pain intensity 73±16 mm), and 90% of them presented at least 1 AE (median of 3 (1 to 6) AEs/patient) with a rate of 5 AEs: 1 ADR without differences due to sex. However, there is a significant delay in referral of female patients (a mean of 6 years) to the Pain Unit, being significantly 3 to 5 times more likely to present sleep or psychiatric disorders. Meanwhile male individuals showed more sexual and reproductive system disorders. Genotypes influenced skin (COMT, G472A-GG) and gastrointestinal (ABCB1, C3435T-CC) related problems.Conclusions:Sex bias affects female patients resulting in a CLBP diagnostic delay and a different analgesic safety profile. Moreover, the individual’s genetic background might be useful to predict certain AEs in opioid-naive patients under an opioid titration procedure. Addressing sex in necessary to resolve inequalities in health care access.
Dantrolene Ameliorates Impaired Neurogenesis and Synaptogenesis in Induced Pluripotent Stem Cell Lines Derived from Patients with Alzheimer’s Disease
Authors: Wang Yong , Liang Ge , Liang Shuqing , Mund Rachel , Shi Yun , Wei Huafeng 
Published: March 2020

Abstract

Background
Overactivation of ryanodine receptors and the resulting impaired calcium homeostasis contribute to Alzheimer’s disease–related pathophysiology. This study hypothesized that exposing neuronal progenitors derived from induced pluripotent stems cells of patients with Alzheimer’s disease to dantrolene will increase survival, proliferation, neurogenesis, and synaptogenesis.

Methods
Induced pluripotent stem cells obtained from skin fibroblast of healthy subjects and patients with familial and sporadic Alzheimer’s disease were used. Biochemical and immunohistochemical methods were applied to determine the effects of dantrolene on the viability, proliferation, differentiation, and calcium dynamics of these cells.

Results
Dantrolene promoted cell viability and proliferation in these two cell lines. Compared with the control, differentiation into basal forebrain cholinergic neurons significantly decreased by 10.7% (32.9 ± 3.6% vs. 22.2 ± 2.6%, N = 5, P = 0.004) and 9.2% (32.9 ± 3.6% vs. 23.7 ± 3.1%, N = 5, P = 0.017) in cell lines from sporadic and familial Alzheimer’s patients, respectively, which were abolished by dantrolene. Synapse density was significantly decreased in cortical neurons generated from stem cells of sporadic Alzheimer’s disease by 58.2% (237.0 ± 28.4 vs. 99.0 ± 16.6 arbitrary units, N = 4, P = 0.001) or familial Alzheimer’s disease by 52.3% (237.0 ± 28.4 vs.113.0 ± 34.9 vs. arbitrary units, N = 5, P = 0.001), which was inhibited by dantrolene in the familial cell line. Compared with the control, adenosine triphosphate (30 µM) significantly increased higher peak elevation of cytosolic calcium concentrations in the cell line from sporadic Alzheimer’s patients (84.1 ± 27.0% vs. 140.4 ± 40.2%, N = 5, P = 0.049), which was abolished by the pretreatment of dantrolene. Dantrolene inhibited the decrease of lysosomal vacuolar-type H+-ATPase and the impairment of autophagy activity in these two cell lines from Alzheimer’s disease patients.

Conclusions
Dantrolene ameliorated the impairment of neurogenesis and synaptogenesis, in association with restoring intracellular Ca2+ homeostasis and physiologic autophagy, cell survival, and proliferation in induced pluripotent stem cells and their derived neurons from sporadic and familial Alzheimer’s disease patients.

Editor’s Perspective

What We Already Know about This Topic

What This Article Tells Us That Is New