bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do Liturgical Vestments Have Gender?
Authors: Leslie Thomas M. 
Published: August 2020

AbstractThis article questions whether traditional Christian liturgical vesture has any intrinsic gendered identity. Vestments are worn by the clergy of various denominations, including in traditions where women are ordained into all orders. For some early female clergy, there was a discomfort about wearing garments traditionally associated solely with male figures, and even today certain vestment manufacturers distinguish between the type of products available for female clergy and for male clergy, or target select gendered clientele. This brief cross-disciplinary examination, of some scriptural, historic and socio-cultural understandings of vesture, concludes that, despite some seeming modern misconceptions to the contrary, vestments are inherently non-gendered, and that they appear predominantly to have been regarded as such at various stages of history. This is consistent with the liturgical understanding that vesture is not meant to be a statement of personal identity, but a symbol of ritual function and office within the gathered assembly.
Revelation and Reason: Theological Epistemology in John Macquarrie’s Thought
Authors: Burge Stephen R. 
Published: August 2020

AbstractJohn Macquarrie’s contribution to Anglican systematic theology has been long acknowledged and his impact is still being felt both within and outside Anglicanism. His existentialist theology, with its roots in German philosophy, as well as Christian mysticism, can at times seem quite distant from ‘traditional’ Anglican theology, but when his way of engaging in theological reflection is examined closely, his epistemology does not appear to be as remote from the ‘traditional’ Anglican hermeneutic of Scripture, tradition reason as it might seem at first sight. This article will argue that Macquarrie’s epistemology is rooted in the Anglican three-fold hermeneutic inherited from Richard Hooker, albeit in a way that is adapted for the modern age. In some respects, Macquarrie’s hermeneutic is a development of Hooker’s ‘three-legged stool’, but a ‘stool’ that has been heavily renovated in light of, and in response to, the existentialist crisis seen in continental philosophy from Søren Kierkegaard onwards. Macquarrie offers a resolution to the tension between individual and corporate identities, and his epistemology may offer Anglican thought a means of negotiating some controversial contemporary theological issues.
Interannual Variability of Lower Equatorial Intermediate Current Response to ENSO in the Western Pacific
Authors: Ma Qiang , Wang Jianing , Wang Fan , Zhang Dongxiao , Zhang Zhixiang , Lyu Yilong 
Published: August 2020

AbstractThe ocean currents of the tropical Pacific Ocean vary with El Niño‐Southern Oscillation (ENSO) cycles. A mooring time series obtained during 2014–2018 in the western Pacific reveal that interannual variability extends to Lower Equatorial Intermediate Current (LEIC). The LEIC velocity anomalies are significantly correlated with the Niño‐3.4 index at an 11‐month lag. Monthly velocity data from the global ocean physical reanalysis product and from a linear continuously stratified ocean model during 1993–2018 capture the 2015–2016 signal and are used to identify the underlying mechanism. During El Niño (La Niña) events, the direct wind forcing in the central tropical Pacific and the reflection of Kelvin waves in the eastern Pacific during the first autumn‐winter period contribute comparably to an eastward (westward) current anomaly in the western Pacific during the second autumn‐winter period. This process is achieved mainly through the generation of the second baroclinic mode and off‐equatorial westward‐propagating downwelling (upwelling) Rossby waves.
The first evidence for Late Pleistocene dogs in Italy
Authors: Boschin Francesco , Bernardini Federico , Pilli Elena , Vai Stefania , Zanolli Clément , Tagliacozzo Antonio , Fico Rosario , Fedi Mariaelena , Corny Julien , Dreossi Diego , Lari Martina , Modi Alessandra , Vergata Chiara , Tuniz Claudio , Moroni Adriana , Boscato Paolo , Caramelli David , Ronchitelli Annamaria 
Published: August 2020

AbstractThe identification of the earliest dogs is challenging because of the absence and/or mosaic pattern of morphological diagnostic features in the initial phases of the domestication process. Furthermore, the natural occurrence of some of these characters in Late Pleistocene wolf populations and the time it took from the onset of traits related to domestication to their prevalence remain indefinite. For these reasons, the spatiotemporal context of the early domestication of dogs is hotly debated. Our combined molecular and morphological analyses of fossil canid remains from the sites of Grotta Paglicci and Grotta Romanelli, in southern Italy, attest of the presence of dogs at least 14,000 calibrated years before present. This unambiguously documents one of the earliest occurrence of domesticates in the Upper Palaeolithic of Europe and in the Mediterranean. The genetic affinity between the Palaeolithic dogs from southern Italy and contemporaneous ones found in Germany also suggest that these animals were an important common adjunct during the Late Glacial, when strong cultural diversification occurred between the Mediterranean world and European areas north of the Alps. Additionally, aDNA analyses indicate that this Upper Palaeolithic dog lineage from Italy may have contributed to the genetic diversity of living dogs.
Whole genome analysis reveals aneuploidies in early pregnancy loss in the horse
Authors: Shilton Charlotte A. , Kahler Anne , Davis Brian W. , Crabtree James R. , Crowhurst James , McGladdery Andrew J. , Wathes D. Claire , Raudsepp Terje , de Mestre Amanda M. 
Published: August 2020

AbstractThe first 8 weeks of pregnancy is a critical time, with the majority of pregnancy losses occurring during this period. Abnormal chromosome number (aneuploidy) is a common finding in human miscarriage, yet is rarely reported in domestic animals. Equine early pregnancy loss (EPL) has no diagnosis in over 80% of cases. The aim of this study was to characterise aneuploidies associated with equine EPL. Genomic DNA from clinical cases of spontaneous miscarriage (EPLs; 14–65 days of gestation) and healthy control placentae (various gestational ages) were assessed using a high density genotyping array. Aneuploidy was detected in 12/55 EPLs (21.8%), and 0/15 healthy control placentae. Whole genome sequencing (30X) and digital droplet PCR (ddPCR) validated results. The majority of these aneuploidies have never been reported in live born equines, supporting their embryonic/fetal lethality. Aneuploidies were detected in both placental and fetal compartments. Rodents are currently used to study how maternal ageing impacts aneuploidy risk, however the differences in reproductive biology is a limitation of this model. We present the first evidence of aneuploidy in naturally occurring equine EPLs at a similar rate to human miscarriage. We therefore suggest the horse as an alternative to rodent models to study mechanisms resulting in aneuploid pregnancies.
A chimeric mouse model to study human iPSC-derived neurons: the case of a truncating SHANK3 mutation
Authors: Vitrac Aline , Pons Stéphanie , Balkota Marta , Lemière Nathalie , Raïs Célia , Bourgeois Jean-Pierre , Maskos Uwe , Bourgeron Thomas , Cloëz-Tayarani Isabelle 
Published: August 2020

AbstractUsing human induced pluripotent stem cells (iPSC), recent studies have shown that the events underlying autism spectrum disorders (ASD) can occur during neonatal development. We previously analyzed the iPSC-derived pyramidal cortical neurons of a subset of patients with ASD carrying de novo heterozygous mutations in postsynaptic SHANK3 protein, in culture. We reported altered spinogenesis of those neurons. The transplantation of human iPSC-derived neuronal precursors into mouse brain represents a novel option for in vivo analysis of mutations affecting the human brain. In this study, we transplanted the neuronal precursor cells (NPC) into the cortex of newborn mice to analyze their integration and maturation at early stages of development and studied axonal projections of transplanted human neurons into adult mouse brain. We then co-transplanted NPC from a control individual and from a patient carrying a de novo heterozygous SHANK3 mutation. We observed a reduction in cell soma size of selective neuronal categories and in axonal projections at 30 days post-transplantation. In contrast to previous in vitro studies, we did not observe any alteration in spinogenesis at this early age. The humanized chimeric mouse models offer the means to analyze ASD-associated mutations further and provide the opportunity to visualize phenotypes in vivo.
A panel of anti-influenza virus nucleoprotein antibodies selected from phage-displayed synthetic antibody libraries with rapid diagnostic capability to distinguish diverse influenza virus subtypes
Authors: Yu Chung-Ming , Chen Ing-Chien , Tung Chao-Ping , Peng Hung-Pin , Jian Jhih-Wei , Chiu Yi-Kai , Tsou Yueh-Liang , Chen Hong-Sen , Huang Yi-Jen , Hsiao Wesley Wei-Wen , Wang Yong Alison , Yang An-Suei 
Published: August 2020

AbstractImmunoassays based on sandwich immuno-complexes of capture and detection antibodies simultaneously binding to the target analytes have been powerful technologies in molecular analyses. Recent developments in single molecule detection technologies enable the detection limit of the sandwich immunoassays approaching femtomolar (10–15 M), driving the needs of developing sensitive and specific antibodies for ever-increasingly broad applications in detecting and quantifying biomarkers. The key components underlying the sandwich immunoassays are antibody-based affinity reagents, for which the conventional sources are mono- or poly-clonal antibodies from immunized animals. The downsides of the animal-based antibodies as affinity reagents arise from the requirement of months of development timespan and limited choices of antibody candidates due to immunodominance of humoral immune responses in animals. Hence, developing animal antibodies capable of distinguishing highly related antigens could be challenging. To overcome the limitation imposed by the animal immune systems, we developed an in vitro methodology based on phage-displayed synthetic antibody libraries for diverse antibodies as affinity reagents against closely related influenza virus nucleoprotein (NP) subtypes, aiming to differentiating avian influenza virus (H5N1) from seasonal influenza viruses (H1N1 and H3N2), for which the NPs are closely related by 90–94% in terms of pairwise amino acid sequence identity. We applied the methodology to attain, within four weeks, a panel of IgGs with distinguishable specificities against a group of representative NPs with pairwise amino acid sequence identities up to more than 90%, and the antibodies derived from the antibody libraries without further affinity refinement had comparable affinity of mouse antibodies to the NPs with the detection limit less than 1 nM of viral NP from lysed virus with sandwich ELISA. The panel of IgGs were capable of rapidly distinguishing infections due to virulent avian influenza virus from infections of seasonal flu, in responding to a probable emergency scenario where avian influenza virus would be transmissible among humans overlapping with the seasonal influenza infections. The results indicate that the in vitro antibody development methodology enables developing diagnostic antibodies that would not otherwise be available from animal-based antibody technologies.
Nonstationary El Niño teleconnection on the post-summer upwelling off Vietnam
Authors: Wang You-Lin , Wu Chau-Ron 
Published: August 2020

AbstractSummer upwelling has often been observed off Vietnam in the South China Sea (SCS). Occasional disappearance of the upwelling has attracted much attention because it modulates the regional climate and harms surrounding fisheries. Fluctuations of the East Asian monsoon associated with El Niño are considered responsible for the weakened or abolished upwelling. However, analyses of observations performed in the present study were equivocal in terms of the dominant influence of El Niño. Based on long-term sea surface temperature data, we demonstrated that weak upwelling off Vietnam occurs more frequently during periods of accelerated global warming compared to warming hiatus periods. Warming signals in the Indian Ocean vanished relatively quickly during the hiatus period. The accompanying easterly anomalies south of the anomalous anticyclone (AAC) in the northwestern Pacific were also weakened, reducing the impact of the El Niño teleconnection on the SCS summer monsoon and thus preserving the regular post-summer upwelling off Vietnam during warming hiatus periods.
Signaling via the p75 neurotrophin receptor facilitates amyloid-β-induced dendritic spine pathology
Authors: Patnaik Abhisarika , Zagrebelsky Marta , Korte Martin , Holz Andreas 
Published: August 2020

AbstractSynapse and dendritic spine loss induced by amyloid-β oligomers is one of the main hallmarks of the early phases of Alzheimer’s disease (AD) and is directly correlated with the cognitive decline typical of this pathology. The p75 neurotrophin receptor (p75NTR) binds amyloid-β oligomers in the nM range. While it was shown that µM concentrations of amyloid-β mediate cell death, the role and intracellular signaling of p75NTR for dendritic spine pathology induced by sublethal concentrations of amyloid-β has not been analyzed. We describe here p75NTR as a crucial binding partner in mediating effects of soluble amyloid-β oligomers on dendritic spine density and structure in non-apoptotic hippocampal neurons. Removing or over-expressing p75NTR in neurons rescues or exacerbates the typical loss of dendritic spines and their structural alterations observed upon treatment with nM concentrations of amyloid-β oligomers. Moreover, we show that binding of amyloid-β oligomers to p75NTR activates the RhoA/ROCK signaling cascade resulting in the fast stabilization of the actin spinoskeleton. Our results describe a role for p75NTR and downstream signaling events triggered by binding of amyloid-β oligomers and causing dendritic spine pathology. These observations further our understanding of the molecular mechanisms underlying one of the main early neuropathological hallmarks of AD.
Thyroid cancer diagnosis by Raman spectroscopy
Authors: Sbroscia Marco , Di Gioacchino Michael , Ascenzi Paolo , Crucitti Pierfilippo , di Masi Alessandra , Giovannoni Isabella , Longo Filippo , Mariotti Davide , Naciu Anda Mihaela , Palermo Andrea , Taffon Chiara , Verri Martina , Sodo Armida , Crescenzi Anna , Ricci Maria Antonietta 
Published: August 2020

AbstractOver the last 50 years, the incidence of human thyroid cancer disease has seen a significative increment. This comes along with an even higher increment of surgery, since, according to the international guidelines, patients are sometimes addressed to surgery also when the fine needle aspiration gives undetermined cytological diagnosis. As a matter of fact, only 30% of the thyroid glands removed for diagnostic purpose have a post surgical histological report of malignancy: this implies that about 70% of the patients have suffered an unnecessary thyroid removal. Here we show that Raman spectroscopy investigation of thyroid tissues provides reliable cancer diagnosis. Healthy tissues are consistently distinguished from cancerous ones with an accuracy of $$\sim $$
∼
90%, and the three cancer typology with highest incidence are clearly identified. More importantly, Raman investigation has evidenced alterations suggesting an early stage of transition of adenoma tissues into cancerous ones. These results suggest that Raman spectroscopy may overcome the limits of current diagnostic tools.
A fully open-source framework for deep learning protein real-valued distances
Authors: Adhikari Badri 
Published: August 2020

AbstractAs deep learning algorithms drive the progress in protein structure prediction, a lot remains to be studied at this merging superhighway of deep learning and protein structure prediction. Recent findings show that inter-residue distance prediction, a more granular version of the well-known contact prediction problem, is a key to predicting accurate models. However, deep learning methods that predict these distances are still in the early stages of their development. To advance these methods and develop other novel methods, a need exists for a small and representative dataset packaged for faster development and testing. In this work, we introduce protein distance net (PDNET), a framework that consists of one such representative dataset along with the scripts for training and testing deep learning methods. The framework also includes all the scripts that were used to curate the dataset, and generate the input features and distance maps. Deep learning models can also be trained and tested in a web browser using free platforms such as Google Colab. We discuss how PDNET can be used to predict contacts, distance intervals, and real-valued distances.
Myeloid-derived suppressor cell depletion therapy targets IL-17A-expressing mammary carcinomas
Authors: Dawod Bassel , Liu Jinghua , Gebremeskel Simon , Yan Chi , Sappong Antonia , Johnston Brent , Hoskin David W. , Marshall Jean S. , Wang Jun 
Published: August 2020

AbstractTriple-negative breast cancer (TNBC) is an invasive subtype of breast cancer but paradoxically associated with increased tumor-infiltrating leukocytes. The molecular and cellular mechanisms underlying TNBC immunobiology are incompletely understood. Interleukin (IL)-17A is a pro-inflammatory cytokine that has both pro- and anti-tumor effects and found in 40–80% of TNBC samples. We report here that IL-17A mRNA and protein are detectable in some human TNBC cell lines and further upregulated by IL-23 and LPS stimulation. Furthermore, the impact of tumor-derived IL-17A in host immune response and tumor growth was examined using murine TNBC 4T1 mammary carcinoma cells transduced with an adenoviral vector expressing IL-17A (AdIL-17A) or control vector (Addl). Compared to Addl-transduction, AdIL-17A-transduction enhanced 4T1 tumor growth and lung metastasis in vivo, which was associated with a marked expansion of myeloid-derived suppressor cells (MDSCs). However, AdIL-17A-transduction also induced strong organ-specific and time-dependent immune activation indicated by dynamic changes of NK cells, B cells, CD4, and CD8 T cells in peripheral blood, lung, and tumor site, as well as the plasma levels of IFNγ. Such findings highlight that tumor-associated IL-17A induces concurrent immune activation and immune suppression. Administration of anti-Gr1 or anti-G-CSF antibody effectively depleted MDSCs in vivo, markedly reducing the growth of AdIL-17A-transduced 4T1 tumors, and eliminating lung metastasis. Collectively, our study demonstrates that MDSC depletion is an effective and practical approach for treating IL-17A-enriched mammary carcinomas.
Optical coherence tomography angiographic findings of lamellar macular hole: comparisons between tractional and degenerative subtypes
Authors: Yeo Joon Hyung , Oh Richul , Lee Joo Yong , Kim June-Gone , Yoon Young Hee , Kim Yoon Jeon 
Published: August 2020

AbstractWe investigated the microvascular changes in eyes with lamellar macular holes (LMHs) using optical coherence tomography angiography (OCTA), compare them between two subtypes of LMH. Tractional and degenerative LMH were differentiated based on the morphological characteristics of OCT. In OCTA images, foveal and parafoveal vessel density (VD) in the superficial and deep capillary plexus (SCP, DCP) and foveal avascular zone (FAZ) area were measured. Eyes that underwent vitrectomy for LMH were included in subgroup analysis. We analysed 63 LMH (42 tractional and 21 degenerative) eyes and 63 control eyes. Compared with degenerative LMH, tractional LMH had better BCVA (p = 0.010), smaller FAZ area (p = 0.001), and higher foveal VD in the SCP (p = 0.130) and DCP (p = 0.002). In degenerative LMH, better BCVA was associated with greater foveal VD in the SCP (p = 0.040) and DCP (p = 0.005), and parafoveal VD in the SCP (p = 0.006). In subgroup analysis, only the tractional LMH group showed significant increases in foveal and parafoveal VDs in the SCP after vitrectomy (p = 0.001 and p = 0.026, respectively). Significant differences in microvascular changes were noted between tractional and degenerative LMH, suggesting that two subtypes are distinct pathogenetic entities.
Ice thickness and volume changes across the Southern Alps, New Zealand, from the little ice age to present
Authors: Carrivick Jonathan L. , James William H. M. , Grimes Michael , Sutherland Jenna L. , Lorrey Andrew M. 
Published: August 2020

AbstractRapid changes observed today in mountain glaciers need to be put into a longer-term context to understand global sea-level contributions, regional climate-glacier systems and local landscape evolution. In this study we determined volume changes for 400 mountain glaciers across the Southern Alps, New Zealand for three time periods; pre-industrial “Little Ice Age (LIA)” to 1978, 1978 to 2009 and 2009 to 2019. At least 60 km3 ± 12 km3 or between 41 and 62% of the LIA total ice volume has been lost. The rate of mass loss has nearly doubled from − 0.4 m w.e year−1 during 1,600 to 1978 to − 0.7 m w.e year−1 at present. In comparison Patagonia has lost just 11% of it’s LIA volume. Glacier ice in the Southern Alps has become restricted to higher elevations and to large debris-covered ablation tongues terminating in lakes. The accelerating rate of ice loss reflects regional-specific climate conditions and suggests that peak glacial meltwater production is imminent if not already passed, which has profound implications for water resources and riverine habitats.
Rapid prototyping of 3D Organic Electrochemical Transistors by composite photocurable resin
Authors: Bertana Valentina , Scordo Giorgio , Parmeggiani Matteo , Scaltrito Luciano , Ferrero Sergio , Gomez Manuel Gomez , Cocuzza Matteo , Vurro Davide , D’Angelo Pasquale , Iannotta Salvatore , Pirri Candido F. , Marasso Simone L. 
Published: August 2020

AbstractRapid Prototyping (RP) promises to induce a revolutionary impact on how the objects can be produced and used in industrial manufacturing as well as in everyday life. Over the time a standard technique as the 3D Stereolithography (SL) has become a fundamental technology for RP and Additive Manufacturing (AM), since it enables the fabrication of the 3D objects from a cost-effective photocurable resin. Efforts to obtain devices more complex than just a mere aesthetic simulacre, have been spent with uncertain results. The multidisciplinary nature of such manufacturing technique furtherly hinders the route to the fabrication of complex devices. A good knowledge of the bases of material science and engineering is required to deal with SL technological, characterization and testing aspects. In this framework, our study aims to reveal a new approach to obtain RP of complex devices, namely Organic Electro-Chemical Transistors (OECTs), by SL technique exploiting a resin composite based on the conductive poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) and the photo curable Poly(ethylene glycol) diacrylate (PEGDA). A comprehensive study is presented, starting from the optimization of composite resin and characterization of its electrochemical properties, up to the 3D OECTs printing and testing. Relevant performances in biosensing for dopamine (DA) detection using the 3D OECTs are reported and discussed too.