bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Detection of sAMY1α in Ovarian Cancer Ascites During First-Line Chemotherapy Predict the Response of Patients to Treatment
Authors: Kong Dan , Liu Yu , Li Junyi , Liu Duanyang , Liu Miao , Qiao Cong , Tang Jing , Zhao Shuang , Lv Haixu , Xing Yanling , Jin Shi , Zhang Lei , Jin Xiaoming 
Published: August 2020

Abstract
Background: Ovarian cancer ascites are a clinical conundrum in the process of diagnosis and treatment, and chemotherapy is the main treatment. The efficacy and prognostic assessment of chemotherapy has been the subject of continuous exploration. Methods: Affymetrix HTA2.0 microarray analysis of ovarian cancer ascites without and with chemotherapy, as well as metabolomics techniques, showed that AMY1A gene expression in the chemotherapy was significantly higher than that without chemotherapy.Results: The analysis of ovarian cancer tissues, ascitic precipitated cells and supernatants, showed that the high expression of sAMY-1α was negatively correlated with HE4 (p<0.01), and was also negatively correlated with the expression of the Beclin1 and LC3 (p<0.05, p<0.001) in group with chemotherapy. The results of ovarian cancer cell lines OVCAR3 and A2780, which were treated with autophagic inhibitor (3MA), inducer (rapamycin) or cisplatin (CDDP), were consistent with it. The results of 3MA group showed that as the drug concentration increased, there were decreased in the levels of Beclin1 and LC3II, and sAMY1α levels increased. There was opposite result in rapamycin group. CDDP group revealed significantly decreased levels of LC3II, Beclin1, and increased levels of sAMY1α; when rapamycin was added, autophagy inhibition was alleviated, and slightly reduced levels of sAMY1α. The results indicated that chemotherapy induced autophagic death of tumor cells, and the expression of sAMY1α in ascites of chemotherapy reflected the degree of autophagic death of tumor cells. Conclusions: During the diagnosis and treatment of ovarian cancer ascites, the expression level of sAMY1α was detected at any time, compared with the levels of HE4, Beclin1 and LC3 in the ascites with chemotherapy, and compared with the serum level of CA125 in the patients, so that the recurrence of ascites could be predicted. Therefore, the adjustment of treatment regimen has important guiding significance.
The enrolment gap: who is not enrolling with primary health organizations in Aotearoa New Zealand and what are the implications? An exploration of 2015-2019 administrative data
Authors: Irurzun-Lopez Maite , Jeffreys Mona , Cumming Jacqueline 
Published: August 2020

Abstract
Background - Primary Health Care (PHC) is the entry point to accessing health services in many countries. Having a high proportion of the population enrolled with a PHC provider is key to ensuring PHC fulfils this role and that it contributes to achieving better equity in health. We aimed to understand the extent to which people in Aotearoa New Zealand are enrolling with Primary Health Organisations (PHOs), how enrolment rates have evolved over time, variations across District Health Boards (DHBs), and socio-demographic groups.Methods - We analysed administrative data on the proportion of people enrolled in PHOs and breakdowns across DHBs, and by age, ethnicity and deprivation, for the years 2015-2019.Results - About 6% of the population was not enrolled in 2019. There are persistent differences across socio-demographic groups as well as geographically. Māori have lower enrolment rates than New Zealand European/Other groups. Young people (15-24 years) are the least likely to be enrolled. The most affluent areas have the highest enrolment rates. Auckland DHB shows the lowest enrolment rates.Conclusions - Enrolments remain below full population coverage and inequities exist between socio-demographic and geographic groups. Potential reasons explaining these trends include methodological limitations as well as real issues in accessing services. We recommend (a) work towards minimising data issues in relation to this indicator to improve its accuracy and value in signalling trends in access to PHC services, and (b) investigating the reasons for the potential widening of the inequities identified, in particular issues preventing Māori and younger people from enrolling. This study deepens our understanding of the enrolment system and its potential pitfalls specially in relation to equity. Other countries can learn from the Aotearoa New Zealand case to draw lessons for improving equity in health care.
WITHDRAWN: Effect of Li2O on Structure and Properties of Glass-Ceramic Bonds
Authors: Guo Zichen , He Feng , Li Zuhao , Yan Dongyang , Zhang Bing , Xie Junlin 
Published: August 2020

Abstract
SiO2-B2O3-Al2O3-CaO vitrified bonds are widely used in the diamond abrasive tools preparation. The effect of Li2O on structure and properties of the glass-ceramic bonds was investigated. The structure of the glass-ceramic bonds was analyzed by XRD and FTIR. The bending strength, the sintering properties of different glass-ceramic bonds and the thermal expansion coefficient were tested at meantime. The sintering and interfacial bonding state between L-4 glass-ceramic bonds and diamond grains were observed by SEM. The results showed that with the increasing of Li2O, the sintering temperature of the glass-ceramic bonds was impactful reducing. When the content of Li2O was 4 wt%, the sintering temperature corresponding to the optimal bending strength was 630 °C, which had a certain reduction compared with other studies. The main crystal phase precipitated in the glass-ceramic bonds was LixAlxSi3−xO6. With the increase of Li2O, the number of crystals in the glass-ceramic bonds gradually increased. The highest bending strength could attain about 136 MPa. Meanwhile, the bending strength performed regular change as the rising of temperature accompanied by the linear shrinkage rate. The L-4 glass-ceramic bonds and diamond grains were found to have very good wetting property and the interfacial bonding strength was insured enhance. The average bending strength of the composite sinter of the glass-ceramic bonds and diamond was up to 87.8 MPa.
Elovanoid-N32 or RvD6-isomer decrease ACE2 and binding of S protein RBD after injury or INFγ in the eye
Authors: Pham Thang L. , He Jiucheng , Kakazu Azucena H. , Calandria Jorgelina , Do Khanh V. , Nshimiyimana Robert , Petasis Nicos A. , Bazan Haydee E.P. , Bazan Nicolas G. 
Published: August 2020

Abstract
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection that causes coronavirus disease 2019 (COVID-19) has resulted in a pandemic affecting the most vulnerable in society, triggering a public health crisis and economic tall around the world. Effective treatments to mitigate this virus infection are needed. Since the eye is a route of virus entrance, we use an in vivo rat model of corneal inflammation as well as human corneal epithelial cells in culture challenged with IFNγ to study this issue. We explore ways to block the receptor-binding domain (RBD) of SARS-CoV-2 spike (S) protein to angiotensin-converting enzyme 2 (ACE2). Elovanoid (ELV)-N32 or Resolvin D6-isomer (RvD6i), among the lipid mediators studied, consistently decreased the expression of the ACE2 receptor, furin, and integrins in damaged corneas or IFNγ stimulated human corneal epithelial cells (HCEC). There was also a concomitant decrease in the binding of spike RBD with the lipid treatments. Concurrently, we uncovered that the lipid mediators also attenuated the expression of cytokines that participate in the cytokine storm, hyper-inflammation and senescence programming. Thus, the bioactivity of these lipid mediators will contribute to opening therapeutic avenues for COVID-19 by counteracting virus attachment and entrance to the eye and other cells and the ensuing disruptions of homeostasis.
Investigation of Quality Assessment for Different Grades of Velvet Antler: a Strategy Combined Microscopic Observation, Delayed Luminescence and Chemical Analysis
Authors: Fan Shasha , Zhu Jianxun , He Min , Wang Nan , Xu Xiaoru , Pang Jingxiang , Yan Yu , Li Li , Yang Jiale , Chang Wen-Te , Chang Tung-Ti , Han Jinxiang , Sun Mengmeng 
Published: August 2020

Abstract
Background

As a famous animal-derived herbal medicine, the pharmacological functions of velvet antler are widely recognized in the world. However, there are still great limitations on quality standard and control system of velvet antler.
Methods

In this research, microscopic observation, delayed luminescence (DL) and chemical analysis were used to evaluate the four grades of velvet antler slices obtained from Cervus nippon Temminck and Cervus elaphus Linnaeu. In addition, those three measurement platforms have been used to compared the two of the four grades among Cervus nippon Temminck, Cervus elaphus Linnaeu and Rangifer tarandus.
Results

We assessed the quality grades of velvet antler slices by using microscopic observation, delayed luminescence and chemical analysis. These techniques are able to indicate the difference of different grades of velvet antler slices to a certain extent, and has their own advantages, respectively.
Conclusion

As a new, rapid and systemic tool, DL provides a technique for studying the overall property of different grades of velvet antler slices, and the integrated assessment by measuring microscopic observation, DL and chemical components may provide a novel means to measure animal-derived herbal quality control.
Under Pressure: Comparing Fish Survivorship Between in Situ and Boat Tagging Methods Using Internal Acoustic Tags
Authors: Keller Jessica A. , Morley Danielle , Herbig Jennifer , Barbera Paul , Feeley Michael W. , Acosta Alejandro 
Published: August 2020

Abstract
Background: With the increase in telemetry studies over the past decade, improving understanding of how different tagging methods influence fish survivorship is critical. By examining the effects of tagging methods, we can maximize the information gained from telemetry studies. Mortality resulting from internally tagging fish on a boat may be due to barotrauma injuries, increased stress from prolonged handling times, or predation after fish have been released back into the water. Conducting in situ internal acoustic tagging at depth of capture completely removes barotrauma stresses and simplifies the release method, which may improve fish survival. In this study, we used 8 years of acoustic tagging data to determine if the tagging method (in situ versus on the boat) influenced fish survivorship and evaluated the role of other tagging variables.Results: At 6 days after tagging, Kaplan–Meier survival curves revealed that the survival probability of fish tagged on the boat was 66% while survival probability of fish tagged in situ was 90%. Tagging method was the only variable to significantly affect survival probability based on Cox proportional hazards models, with fish tagged in situ ~75% less likely to have an “event” (mortality, tag loss, or emigration) compared to fish tagged on the boat at both 4 and 6 days after tagging. Examining tagging methods separately, handling time only marginally influenced survival probability of boat-tagged fish and no variables had a significant effect on survival of in situ tagged fish. Conclusions: In this study, tagging method was the only variable to significantly affect survival of internally tagged fish. Implanting internal acoustic tags in situ is not a practical method for every species and for every environment, but given the increased fish survivorship demonstrated here, we strongly suggest it be considered as the preferred tagging methodology where applicable.
Community groups to increase Human papillomavirus (HPV) vaccination through Participatory Learning and Action, Reducing barriers and increasing community Mobilisation in Zambia (CHARMZ) project.
Authors: Hoernke Katarina 
Published: August 2020

Abstract
Background Zambia has a high incidence of cervical cancer. Uptake of the human papillomavirus (HPV) vaccine has been low since it first became available in Zambia in 2013. Community mobilisation and engagement with civil society has been identified as a key component of HPV vaccination programs in low and middle-income countries. Of the evidence available on community-based interventions in the context of HPV, participatory approaches which work closely with local stakeholders to identify and overcome barriers to HPV vaccination uptake have shown promising results. This is a proposal for a community-based cluster randomised controlled trial to increase HPV vaccination uptake in Zambia through a participatory learning and action (PLA) cycle approach in community groups. PLA is an approach in which participants identify and prioritise problems, and subsequently develop, implement and evaluate solutions to improve the health needs of their community. Key stakeholders to be engaged in this project include government members, policymakers, healthcare, donors and relevant non-governmental organisations (NGO), religious leaders, and civil society members. Formative research will be carried out with key stakeholders through semi-structured interviews and focus group discussions. The community group intervention will be facilitated by local healthcare workers from the cervical cancer prevention program in Zambia (CCPPZ).Results Success will be indicated by an increase in HPV vaccination uptake. The primary outcome will be HPV vaccination rates measured quantitatively. Data will be gathered once at the start of the study, once midway through the PLA phase and once during the post-intervention phase. Questionnaires and semi-structured interviews will allow for measurement of secondary outcomes both quantitatively and qualitatively, including perceived (i) HPV vaccination and cervical cancer knowledge, (ii) acceptability, accessibility and equitability of the HPV vaccination, as well as (iii) attitudes of female group members towards other cervical cancer screening and treatment services.Conclusions There is a need to address the high cervical cancer disease burden in Zambia. This would be the first intervention aiming to increase HPV vaccination uptake in Zambia through a low-cost, sustainable and scalable community-based participatory learning and action cycle approach.
Value of laboratory tests in COVID-19 hospitalized patients for clinical decision-makers: a predictive model, using data mining approach
Authors: Mousavi Atefeh , Rezaei Soheyla , Salamzadeh Jamshid , Mirzazadeh Ali , Peiravian Farzad , Yousefi Nazila 
Published: August 2020

Abstract
Purpose: Because of the rapid increase in confirmed cases of COVID-19, in particular those with severe or critical status, overwhelming of health systems is a worldwide concern. Therefore, identifying high-risk COVID-19 patients, can help service providers for priority setting and hospital resource allocation. Methods: 4542 adult patients with confirmed COVID-19 admitted in 15 hospitals in Tehran, Iran, from Feb 20 to April 18, 2020 were included in this retrospective cohort study with final outcomes of survived and died patients. Demographic features including age and sex, and laboratory data measured at admission were extracted and compared between recovered and died patients. Data analysis was performed applying SPSS modeler software using a logistic regression method.Results: Of 4542 hospitalized adult patients, 822 patients (18.09%) died during hospitalization, and 3720 (81.90%) recovered and discharged. Based on logistic regression model, older age, 40-49 (RR= 1.80, CI: 1.13-2.87), 50-59 (RR=2.63, CI: 1.71-4.02), 60-69 (RR= 4.40, CI: 2.92-6.63), 70-79 (RR=7.49, CI: 5.01-11.19), Above 80 (RR=13.85, CI: 9.23-2.77), ALT ≥ 55 IU/ (RR=2.20, CI: 1.69-2.86), AST ≥ 100 IU/L (RR=5.93, CI: 4.75-7.39), ALP ≥ 200 IU/L (RR=2.46, CI: 1.80-3.37), sodium < 135 mEq/l (RR=1.69, CI: 1.35-2.11) or more than 145 mEq/l (RR=7.24, CI: 5.07-10.33), potassium > 5.50 mEq/l (RR=7.53, CI: 4.15-13.64), and calcium < 8.50 mEq/l (RR=3.39, CI: 2.81-4.09), CPK between 307-600 IU/L (RR=2.73, CI: 2.12-3.53) and above 600 IU/L (RR=4.41, CI: 3.40-5.71) in men, and 192-400 IU/L (RR=2.73, CI: 2.12-3.53), and above 400 (RR=4.41, CI: 3.40-5.71) in women, CRP > 3 mg/l (RR=3.22, CI: 1.99-5.20), and creatinine > 1.5 mg/l (RR=6.37, CI: 5.30-7.66) were significantly associated with COVID-19 mortality. Conclusion: Our findings suggested less than one in five hospitalized patients with COVID-19 die mostly due to electrolyte disbalance, liver, and renal dysfunctions. Better supportive care is needed to improve outcomes for patients with COVID-19.
Two novel QTLs for harvest index contribute to high yield production in rice (Oryza sativa L.)
Authors: Saito Hiroki , Fukuta Y. , Obara Mitsuhiro , Tomita Asami , Ishimaru Tsutomu , Sasaki Kazuhiro , Fujita Daisuke , Kobayashi Nobuya 
Published: August 2020

Abstract
Background: Harvest index (HI) is a measure of the biological success of forming harvestable products. However, our understanding of the genetic basis of HI in rice (Oryza sativa L.) is limited, because it is a complex trait consisting of various yield-related traits and physiological attributes. YTH183 is a high yielding line with large panicles and high HI derived from a cross between the Indica Group variety IR 64 and the NPT line IR 69093-41-2-3-2 (YP5).Results: Here, we detected two novel QTLs for HI, designated qHI5.1 on chromosome 5 and qHI8.1 on chromosome 8. The YTH183 allele at qHI5.1 contributed to wide grain, resulting in heavy grain weight and panicle weight, and was consistently effective under the different environmental conditions of subtropical (Ishigaki) and temperate (Tsukuba) regions. Genetic polymorphism revealed that qHI5.1 was identical to GSE5/GW5, which is known to control the grain weight. On the other hand, although qHI8.1 functioned additively with qHI5.1 for higher HI, it did not show any significant effect on grain or panicle weight. In addition, its effects on HI were shown only in the first seasons at Ishigaki but not at Tsukuba or in the second season at Ishigaki. Conclusion: Our results indicate that qHI5.1 controls the grain size, regardless of whether environmental conditions are of subtropical or temperate regions, while qHI8.1 might be involved in controlling the physiological processes of source ability or the translocation of photosynthesis products from vegetative organs to grains depending on environmental conditions during the maturing stage. These QTLs will be useful genetic resources for future breeding programs to break through the ceiling of maximum yield in Indica Group varieties.
High-pressure and high-temperature vibrational properties and anharmonicity of carbonate minerals up to 6 GPa and 500 °C by Raman spectroscopy
Authors: Farsang Stefan , Widmer Remo N. , Redfern Simon A.T. 
Published: August 2020

AbstractCarbonate minerals play a dominant role in the deep carbon cycle. Determining the high-pressure and high-temperature vibrational properties of carbonates is essential to understand their anharmonicity and their thermodynamic properties under crustal and upper mantle conditions. Building on our previous study on aragonite, calcite (both CaCO3 polymorphs), dolomite [CaMg(CO3)2], magnesite (MgCO3), rhodochrosite (MnCO3), and siderite (FeCO3) (Farsang et al. 2018), we have measured the pressure- and temperature-induced frequency shifts of Raman-active vibrational modes up to 6 GPa and 500 °C for all naturally occurring aragonite- and calcite-group carbonate minerals, including cerussite (PbCO3), strontianite (SrCO3), witherite (BaCO3), gaspeite (NiCO3), otavite (CdCO3), smithsonite (ZnCO3), and spherocobaltite (CoCO3). Our Raman and XRD measurements show that cerussite decomposes to a mixture of Pb2O3 and tetragonal PbO between 225 and 250 °C, smithsonite breaks down to hexagonal ZnO between 325 and 400 °C, and gaspeite to NiO between 375 and 400 °C. Spherocobaltite breaks down between 425 and 450 °C and otavite between 375 and 400 °C. Due to their thermal stability, carbonates may serve as potential reservoirs for several metals (e.g., Co, Ni, Zn, Cd) in a range of crustal and upper mantle environments (e.g., subduction zones). We have determined the isobaric and isothermal equivalents of the mode Grüneisen parameter and the anharmonic parameter for each Raman mode and compare trends in vibrational properties as a function of pressure, temperature, and chemical composition with concomitant changes in structural properties. Finally, we use the anharmonic parameter to calculate the thermal contribution to the internal energy and entropy, as well as the isochoric and isobaric heat capacity of certain carbonates.
Ligand-Linked Association-Dissociation in Transport Proteins and Hormone Receptors
Authors: Saccoccia Fulvio , Bellelli Andrea 
Published: August 2020

:
Ligand-linked changes in the aggregation state of biological macromolecules occur and
have importance in several physiological processes, e.g., the response of hormone receptors, cooperative
ligand binding, and others. The mathematical formalisms that express the thermodynamics governing
these processes are complex, as they are required to describe observations made under experimental
conditions in which many parameters may be simultaneously varied. The description of the
functional behaviour of proteins that present ligand-linked association-dissociation events must accommodate
cases where both the binding stoichiometries and reaction mechanisms are variable. In this
paper, we review some paradigmatic cases that cover different structural arrangements and binding
modes, with special attention to the case of dissociating homodimeric transport proteins and receptors.
Even though we cannot pretend to be comprehensive on the proteins presenting this behaviour, we
believe that we can attempt to be comprehensive on the structural arrangements and thermodynamic
properties of these systems, which fall into a limited set of possible types.
Spatio-temporal Distribution of Meningitis in HIV Patients in Northern Egypt (2000-2018)
Authors: Abd El-Wahab Ekram W. , Hegazy Yamen , Farrag Talaat , Metwally Mohammed 
Published: August 2020

Background:Meningitis is a leading cause of death among patients living with HIV. There is no adequate tracking of the disease occurrence, distribution and etiologic agents among this risk group in Egypt, although the pattern could differ than that of the general population.Objectives:We aimed to describe the spatio-temporal distribution of meningitis in HIV patients in a region of Northern Egypt over an 18-years period (2000-2018).Methods:We conducted a retrospective study of 352 adults HIV patients admitted to a tertiary care fever hospital with neurological manifestations suggesting meningitis. We retrieved from inpatient records all data relevant to patient demographics, clinical presentation, diagnostic work-up, results of laboratory investigations (CSF, blood, imaging), definitive diagnosis, and in-hospital mortality.Results:The overall trend over 2 decades showed fluctuating incidence of meningitis in HIV infected patients and increasingly spread into rural areas, with a uniform circulation among adult males. Cryptococcal meningitis was the most common etiologic agent (26.9%) and was associated with worse outcomes. Focal neurological deficit (38.5%), cranial nerve involvement (48.1%) were common features in TB Meningitis. The mortality was high (56.8%) and was significantly associate with low CD4+ count, advanced AIDs clinical stage and the presence of co-morbidities.Conclusion:Despite the availability of cART, meningitis particularly cryptococcal is common in HIV/AIDS population in Egypt. Continued efforts are desperately needed to improve outcomes of HIV-infected patients.