bluuebunny/crossref_metadata_2025_split huggingface dataset:
P53 in RhoA regulation
Authors: Barabutis Nektarios 
Published: February 2020

AbstractRecent evidence suggest that dysregulation of the tumor suppressor P53, in combination with disharmonious activities of the small GTPase RhoA, may lead to irreversible progression of human disease. The purpose of the present study is to communicate the most recent information regarding the highly interrelated P53/RhoA network. Laborious investigations in the association between both components, may reveal new targets for opposing severe human disease, including the acute respiratory distress syndrome.
Rates of Ring Opening of Radical Cation Intermediates Govern Differences in Thermoluminescence between 1‐ and 2‐Naphthyl‐Substituted Methylenecyclopropanes
Authors: Matsui Yasunori , Shimono Kazuki , Takae Kosuke , Namai Hayato , Sera Toshiki , Ogaki Takuya , Ohta Eisuke , Mizuno Kazuhiko , Ikeda Hiroshi 
Published: February 2020

AbstractThe front cover artwork is provided by the group of Prof. Dr. Hiroshi Ikeda in Osaka Prefecture University (Japan). The picture represents the difference in reactivity between 1‐ and 2‐naphthyl‐substituted methylenecyclopropanes. The weak bond in the former substance can be broken even by a small samurai resulting in pink fluorescence, whereas the strong bond in the latter cannot be broken even by a big samurai (resulting in green phosphorescence). Read the full text of the Communication at 10.1002/cptc.201900230.
Modeling and experimental validation of parasitic capacitance effects on emulated bioimpedance measurements with high‐impedance residuals
Authors: Mathews Robert Joseph , Freeborn Todd J. 
Published: February 2020

SummaryParasitic capacitances and high‐residual impedances (typical of electrode/tissue interfaces) have significant effects on estimated electrical impedances of tissues under study. This leads to deviations (especially at high frequency) from the true tissue impedance. This work outlines the circuit theory describing estimation errors occurring when one and two parasitic capacitances are present in tetrapolar electrode configurations typical for bioimpedance measurements. The described circuit theory was validated using both simulations and measurements of a fabricated printed circuit board that implemented the tetra‐polar model. Using the presented model, correction procedures using (a) measurements of the source and sink excitation currents and (b) averaging measurements of forward and reverse electrode configurations have been validated in simulation showing reductions in the estimation errors at frequencies greater than 1 MHz.
Low‐frequency mechanical vibration induces apoptosis of A431 epidermoid carcinoma cells
Authors: Anggayasti Wresti L. , Imashiro Chikahiro , Kuribara Taiki , Totani Kiichiro , Takemura Kenjiro 
Published: February 2020

AbstractCancer research is increasingly focused on discovering strategies to induce cancer cell apoptosis without affecting surrounding normal cells. One potential biocompatible method is mechanical vibration, which has been developed as part of the emerging field of mechanomedicine. Previous studies of mechanical vibration have employed high‐frequency vibration, which damages healthy cells. In this study, we examined the effects of brief (1 h) low‐frequency (20 Hz) mechanical vibration on glucose consumption and survival (apoptosis, necrosis, HMGB1 release) of the human epidermoid carcinoma cell line A431. We found that apoptosis, but not necrosis, was significantly increased at 48 h after mechanical vibration compared with cells maintained in static culture. In keeping with this, extracellular release of HMGB1, a necrosis marker, was lower in cultures of A431 cells subjected to mechanical vibration compared with control cells. Glucose consumption was increased in the first 24 h after mechanical vibration but returned to control levels before the onset of apoptosis. Although the precise intracellular mechanisms by which low‐frequency mechanical vibration triggers apoptosis of A431 cells is unknown, these results suggest a possible role for metabolic pathways. Mechanical vibration may thus represent a novel application of mechanomedicine to cancer therapy.
A Markovian reliability approach for offshore wind energy system analysis in harsh environments
Authors: Adumene Sidum , Okoro Aghatise 
Published: February 2020

AbstractFor an effective monitoring strategy of offshore energy systems in harsh environments, it is vital the system reliability dynamics be fully understood. This article presents the application of a Markovian process for dynamic reliability prediction of an offshore energy system. In the proposed approach, a three‐state Markovian process is developed for the analysis of the subsystem states, which dynamically determines the overall system reliability performance, overcoming the static limitation of fault tree analysis. The multistate subsystems are discretized into normal, degraded, and failed states to demonstrate the performance dynamics of the system for condition monitoring. The proposed approach is tested on a case study and the most critical influencing elements are identified. The application of the methodology efficiently identifies the system vulnerability path so as to assist in system integrity management and to provide a guide to early remedial action against total failure and downtime in offshore energy generation.
On the forecasting of high‐frequency financial time series based on ARIMA model improved by deep learning
Authors: Li Zhenwei , Han Jing , Song Yuping 
Published: February 2020

AbstractThrough empirical research, it is found that the traditional autoregressive integrated moving average (ARIMA) model has a large deviation for the forecasting of high‐frequency financial time series. With the improvement in storage capacity and computing power of high‐frequency financial time series, this paper combines the traditional ARIMA model with the deep learning model to forecast high‐frequency financial time series. It not only preserves the theoretical basis of the traditional model and characterizes the linear relationship, but also can characterize the nonlinear relationship of the error term according to the deep learning model. The empirical study of Monte Carlo numerical simulation and CSI 300 index in China show that, compared with ARIMA, support vector machine (SVM), long short‐term memory (LSTM) and ARIMA‐SVM models, the improved ARIMA model based on LSTM not only improves the forecasting accuracy of the single ARIMA model in both fitting and forecasting, but also reduces the computational complexity of only a single deep learning model. The improved ARIMA model based on deep learning not only enriches the models for the forecasting of time series, but also provides effective tools for high‐frequency strategy design to reduce the investment risks of stock index.
The Patatin‐Like Phospholipase Domain Containing Protein 7 Facilitates VLDL Secretion by Modulating ApoE Stability
Authors: Wang Xiuyun , Guo Min , Wang Qian , Wang Qingjie , Zuo Shasha , Zhang Xu , Tong Hui , Chen Jizheng , Wang Huimin , Chen Xiaowei , Guo Junyuan , Su Xiong , Liang Hui , Zhou Hongwen , Li John Zhong 
Published: February 2020

Background and Aims
The regulation of hepatic very‐low‐density lipoprotein (VLDL) secretion is vital for lipid metabolism whose pathogenetic status is involved in fatty liver disease and dyslipidemia seen in hepatic steatosis. Accumulated evidence suggest that apolipoprotein E (ApoE) is closely related to hepatic VLDL secretion. Here, we report that the expression of patatin‐like phospholipase domain containing protein 7 (PNPLA7) is strongly induced by hepatic steatosis and positively correlates with plasma triacylglycerol (TAG) levels in the human subjects, whereas the role of PNPLA7 in hepatic VLDL secretion is unknown.

Approach and Results
Herein, with genetic manipulation in the mice, the deficiency of hepatic PNPLA7 expression resulted in reduced VLDL secretion accompanied by enhanced hepatic lipid accumulation and decreased hepatic ApoE expression. Furthermore, knockdown of PNPLA7 in the livers of the db/db mice also resulted in significant reduction in plasma TAG level but aggravated hepatic steatosis. Importantly, we observed that PNPLA7 interacted with ApoE and presumably at the site of endoplasmic reticulum. Mechanistically, we have shown that PNPLA7 could modulate polyubiquitination and proteasomal‐mediated degradation of ApoE. Overexpressed ApoE restored the impaired VLDL‐TAG metabolism in PNPLA7‐knockdown primary hepatocytes.

Conclusion
PNPLA7 plays a critical role in regulating hepatic VLDL secretion by modulating ApoE stability through its interaction with ApoE.
The distribution of hepatitis B virus surface antigen polymorphisms at positions associated with vaccine escape
Authors: Raheel Mahad , Choga Wonderful T. , Blackard Jason T. 
Published: February 2020

AbstractHepatitis B virus (HBV) infects over 250 million people worldwide. Vaccination is effective at preventing infection, although several mutations within the “a” determinant region of the HBV surface antigen (HBsAg) are associated with vaccine escape. We evaluated the frequency, genotype, and global distribution of polymorphisms at sites associated with vaccine escape in 4244 unique full‐length HBV genomes. The “a” determinant within the Surface gene was inspected for polymorphisms at sites identified previously associated with vaccine escape. Nearly, 268 (6.3%) sequences from 36 countries contained a polymorphism at a site associated with vaccine escape including 22 genotype A, 99 genotype B, 93 genotype C, 32 genotype D, 14 genotype E, 3 genotype F, 2 genotype G, and 3 genotype I. In genotype A, the most common polymorphism occurred at M133. In genotype B, Q129 and M133 occurred 45 and 51 times, respectively, accounting for 94% of polymorphisms. Polymorphisms at G145 were most frequent in genotype C, while P120 was most common in genotype D. Among all genotypes, polymorphisms at M133 were the most common and accounted for 30.9% of polymorphisms. Polymorphisms at T116, P120, F134, K141, and P142 occurred in geographically diverse locations, whereas polymorphisms at Q129, M133, D144, and G145 were concentrated in East Asia. While the sample size is large, this approach relied on convenience sampling within each country, and many countries have no data available, thereby highlighting the need for additional routine surveillance of surface antigen mutations associated with vaccine escape.
Evolutionary history, potential intermediate animal host, and cross‐species analyses of SARS‐CoV‐2
Authors: Li Xingguang , Zai Junjie , Zhao Qiang , Nie Qing , Li Yi , Foley Brian T. , Chaillon Antoine 
Published: February 2020

AbstractTo investigate the evolutionary history of the recent outbreak of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in China, a total of 70 genomes of virus strains from China and elsewhere with sampling dates between 24 December 2019 and 3 February 2020 were analyzed. To explore the potential intermediate animal host of the SARS‐CoV‐2 virus, we reanalyzed virome data sets from pangolins and representative SARS‐related coronaviruses isolates from bats, with particular attention paid to the spike glycoprotein gene. We performed phylogenetic, split network, transmission network, likelihood‐mapping, and comparative analyses of the genomes. Based on Bayesian time‐scaled phylogenetic analysis using the tip‐dating method, we estimated the time to the most recent common ancestor and evolutionary rate of SARS‐CoV‐2, which ranged from 22 to 24 November 2019 and 1.19 to 1.31 × 10−3 substitutions per site per year, respectively. Our results also revealed that the BetaCoV/bat/Yunnan/RaTG13/2013 virus was more similar to the SARS‐CoV‐2 virus than the coronavirus obtained from the two pangolin samples (SRR10168377 and SRR10168378). We also identified a unique peptide (PRRA) insertion in the human SARS‐CoV‐2 virus, which may be involved in the proteolytic cleavage of the spike protein by cellular proteases, and thus could impact host range and transmissibility. Interestingly, the coronavirus carried by pangolins did not have the RRAR motif. Therefore, we concluded that the human SARS‐CoV‐2 virus, which is responsible for the recent outbreak of COVID‐19, did not come directly from pangolins.
Temperature dependence of strain–phonon coefficient in epitaxial Ge/Si(001): A comprehensive analysis
Authors: Manganelli C.L. , Virgilio M. , Skibitzki O. , Salvalaglio M. , Spirito D. , Zaumseil P. , Yamamoto Y. , Montanari M. , Klesse W.M. , Capellini G. 
Published: February 2020

AbstractWe investigate the temperature dependence of the Ge Raman mode strain–phonon coefficient in Ge/Si heteroepitaxial layers. By analyzing the temperature‐dependent evolution of both the Raman Ge─Ge line and of the Ge lattice strain, we obtain a linear dependence of the strain–phonon coefficient as a function of temperature. Our findings provide an efficient method for capturing the temperature‐dependent strain relaxation mechanism in heteroepitaxial systems. Furthermore, we show that the rather large variability reported in the literature for the strain–phonon coefficient values might be due to the local heating of the sample due to the excitation laser used in μ‐Raman experiments.
Improvement of texture properties and syneresis of arrowroot (Maranta arundinacea) starch gels by using hydrocolloids (guar gum and xanthan gum)
Authors: da Silva Costa Rafael Alves , Bonomo Renata Cristina Ferreira , Rodrigues Luciano Brito , Santos Leandro Soares , Veloso Cristiane Martins 
Published: February 2020

AbstractBACKGROUNDThe incorporation of hydrocolloids into starch dispersions modifies their techno‐functional properties, such as gelatinization, retrogradation, syneresis, and texture, among others. Their main function is to improve these properties and to promote greater stability of starch gels. Thus, the main objective of this study was to evaluate the effect of adding colloids (guar gum and xanthan gum) on the texture properties (hardness, elasticity, cohesiveness, and gumminess) and syneresis of the starch gels made from the common variety of arrowroot. Analysis of variance (ANOVA) and regression were carried out to analyze the effects of the treatments and variables with their respective interactions.RESULTSThe addition of guar gum and xanthan gum influenced the stability of the starch gels studied, and it was capable of reducing syneresis even at low concentrations, with a greater effect for xanthan gum. Both gums were capable of inhibiting syneresis at concentrations above 0.5%, throughout the storage time studied (5 days). The addition of these hydrocolloids was also shown to influence the following texture parameters: hardness, cohesiveness, and gumminess, but showed no effect on gel elasticity.CONCLUSIONThe addition of hydrocolloids was shown to be an alternative way of increasing the stability and enhancing the textural properties of the starch gels in arrowroot. © 2020 Society of Chemical Industry
Topical capsaicin cream with moderate exercise protects against hepatic steatosis, dyslipidemia and increased blood pressure in hypoestrogenic obese rats
Authors: de Lourdes Medina‐Contreras Juana Maria , Mailloux‐Salinas Patrick , Colado‐Velazquez Juventino III , Gómez‐Viquez Norma Leticia Gomez Viquez , Velázquez‐Espejel Rodrigo , del Carmen Susunaga‐Notario Ana , Arias‐Chávez David Julian , Bravo Guadalupe 
Published: February 2020

AbstractBACKGROUNDThe aim of this study was to evaluate the effects of capsaicin (Cap), moderate exercise (Ex), and their combination on arterial blood pressure (BP) and metabolic complications in hypoestrogenic (HE) obese (HEOb) rats. Female Wistar rats were ovariectomized and given 300 g L−1 sucrose solution (HEOb), or purified water (HE) ad libitum, for 28 weeks. After shaving the abdominal skin, cold cream vehicle was applied to sedentary (Sed) and exercise (Ex) groups, and 0.75 g kg−1 Cap cream was applied to Ex groups. Ex groups ran on a treadmill every day for 20 min at speeds from 0.15 to 0.3 m s−1. For combination groups (Cap + Ex), topical Cap was applied 90 min before Ex. The treatments were performed for 6 weeks, and BP was recorded before and at the end of the experimental protocol. The animals were killed by decapitation, and blood and tissues were obtained to perform oxidative profile, as well as to undertake biochemical and histologic studies.RESULTSCompared with individual treatments, the combined therapy (Cap + Ex) in HEOb rats caused a higher reduction in the caloric intake, body weight, abdominal fat percentage, oxidative stress, and hepatic steatosis. In HEOb groups, Cap was the only treatment that reduced BP and prevented dyslipidemia and oxidative stress.CONCLUSIONThe present data show that Cap improves the metabolic alterations induced by obesity and hypoestrogenism, suggesting that Cap can be considered as an excellent candidate for therapy of these clinical conditions. © 2020 Society of Chemical Industry
Real‐time near‐infrared fluorescence imaging mediated by blue dye in breast cancer patients
Authors: Jiang Lili , Liu Taiyuan , Wang Xuan , Li Jun , Zhao Haidong 
Published: February 2020

AbstractBackgroundNear‐infrared (NIR) fluorescence imaging has recently been introduced to the sentinel lymph node (SLN) mapping because of the benefits of the SLN biopsy, such as providing real‐time and high‐resolution optical guidance. Methylene blue is available and less expensive as an SLN mapping tracer. Our study aims to identify SLN through the NIR fluorescence imaging system mediated by blue dye.MethodsEarly‐stage breast cancer patients were prospectively enrolled. All participants received a subareolar or peritumoral injection of 1 mL methylene blue (MB) before surgery. The MB fluorescence system was set immediately after injection. SLNs were searched and removed under the guidance of fluorescence and blue dye.ResultsWe identified SLN adequately with the help of real‐time lymphography and blue dye. Symbolic lymphatic drainage patterns were also observed.ConclusionNIR fluorescence imaging mediated by blue dye has benefits on the identification of lymph vessels, the location of SLN, and the patterns of breast lymphatic flow.
Hexagonal ring electromagnetic band gap‐based slot antenna for circular polarization and performance enhancement
Authors: Verma Alka , Singh Anil Kumar , Srivastava Neelam , Patil Shilpee , Kanaujia Binod Kumar 
Published: February 2020

AbstractIn this article, a hexagonal ring electromagnetic band gap (HREBG) structure‐based circularly polarized (CP) slot antenna for improving performance is proposed. The proposed antenna has a compact structure with a low profile of 0.517λo × 0.517λo × 0.055λo (λo stands for the free space wavelength at 5.10 GHz), is coplanar waveguide fed, and comprises of a circular microstrip patch with a T‐shaped slot. An inclined coupling slot loaded feed line is used on the ground for the generation of CP. Furthermore, eight HREBG's are implemented on the substrate to enhance the performance of the antenna, thereby achieving a measured impedance bandwidth of 18%, 3‐dB axial ratio bandwidth of 6.4%, and a peak gain of 5.93 dBi in the operating bandwidth. A close agreement is reached between the experimental results and the simulated results to verify the proposed antenna's performance.
Post‐Procedure Protocols Following Platelet‐Rich Plasma Injections for Tendinopathy: A Systematic Review
Authors: Townsend Christine , Von Rickenbach Kristian J. , Bailowitz Zachary , Gellhorn Alfred C. 
Published: February 2020

ObjectivePlatelet‐rich plasma (PRP) has been increasingly studied as a treatment for tendinopathy. Many factors may influence outcomes after PRP, including different protocols following administration. It was hypothesized that there would be heterogeneity in post‐PRP protocols.Literature SurveyA systematized review of the literature on post‐PRP protocols for tendinopathy was conducted using an electronic search of MEDLINE and Embase databases through September 2018.MethodologyAfter duplicates were removed, English language articles involving adult patients who received PRP for tendinopathy were reviewed. Exclusion criteria included studies with fewer than 10 patients, PRP used to treat pathology other than tendinopathy, multiple protocols in one study, and surgical settings. Protocol specifics were extracted including nonsteroidal anti‐inflammatory drugs (NSAID) restrictions before and after injection, postinjection restrictions on movement and weight bearing, use of orthoses, activity modifications, and postinjection rehabilitation protocols. Given limitations in the data, a meta‐analysis was not performed.SynthesisEighty‐four studies met inclusion criteria. Following PRP injection, weight‐bearing restrictions were mentioned rarely (12% of protocols). Orthosis use was uncommon overall (18%) but more common in Achilles tendinopathy protocols (53%). The majority of protocols instituted a period of stretching (51%) and strengthening (54%). Stretching programs generally began 2‐7 days following injection, and strengthening programs began within 2‐3 weeks. Preinjection NSAID restriction was reported rarely (20%), whereas postinjection NSAID restriction was more common (56%), with a typical restriction of greater than 2 weeks (38%). Return to play or full activity was reported in 42% of protocols, most commonly at 4‐6 weeks following injection.ConclusionAlthough the clinical effectiveness of PRP remains controversial, even less is known about the effect of post‐PRP protocols, which may affect the outcomes attributed to PRP itself. No studies directly compare post‐PRP protocols, and the protocols studied demonstrate substantial heterogeneity. Some consensus regarding post‐PRP protocols exists, although the rationale for these recommendations is limited.
Synthesis of Double‐Layered NiCo2O4‐Nanosheet‐Loaded PAN/Lignin‐Based Hollow Carbon Nanofibers for High‐Performance Supercapacitor
Authors: Yang Xiaoxiao , Mao Linchang , Peng Wei , Jin Junhong , Yang Shenglin , Li Guang 
Published: February 2020

AbstractPolyacrylonitrile (PAN) /lignin based hollow carbon nanofibers (HCNF) were fabricated by coaxial electrospinning method followed by carbonization procedure. The results show that the NiCo2O4 oxides were successfully deposited on the surface of HCNF by a facile hydrothermal method without any toxic reagents and NiCo2O4 nanosheets are uniformly deposited on the inner and outer layers of HCNF. The electrochemical results show that NiCo2O4@HCNF composite had excellent electrochemical properties. The electrochemical measurements indicate that the NiCo2O4@HCNF possessed considerable capacitance 1998.18 mF/cm2 at 2 mA/cm2 (1665 F/g with the mass loading of 1.2 mg/cm2) and an excellent cycling stability, specific capacitance can retain 87% after 5000 cycle numbers under the current density of 10 mA/cm2. In addition, an asymmetric supercapacitor (NiCo2O4@HCNF//active carbon (AC)) was assembled by NiCo2O4@HCNF (positive electrode) and AC (negative electrode). The device exhibits high capacitance 111 F/g at 1 A/g, excellent energy density (40.3 Wh/kg at 810W/kg), and excellent cycling stability (92% capacitance can retain after 5000 charge‐discharge at 5 A/g). The experimental results show that the hollow structure of the hollow carbon nanofiber benefits the double layered growth of NiCo2O4, which can improve the capacitance performance. Therefore, the composite exhibits excellent electrochemical properties.