bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSIV-B-42 Late-Breaking: Effects of dietary inactivated probiotics on growth performance, nutrient digestibility, and immune responses of weaned pigs
Authors: Kang Joowon , Chae Jong Pyo , Kim S-H , Kim J-W , Park Sangwoo , Mun Daye , Kim Byeonghyeon , Kim Sheena , Lee Jeongjae , Kim Soyun , Kyoung Hyunjin , Choe Jeehwan , Song Minho 
Published: December 2019

Abstract
The study was conducted to investigate effects of dietary inactivated probiotics on growth performance, nutrient digestibility, and immune responses of weaned pigs. A total of 96 weaned pigs (initial BW = 6.95 ± 0.25 kg) were randomly assigned to 4 dietary treatments with increasing levels of inactive probiotics (4 pigs/replicate; 6 replicates/treatment; 0, 0.1, 0.2, and 0.4%; CON, T1, T2, and T3) in a randomized complete block design (BW and sex as blocks). The inactivated probiotics used in this experiment was a commercial product (CJ CheilJedang Biotechnology Research Institute, Seoul, Korea) containing 1 x 106 CFU/g on stains of Lactobacillus rhamnosus. Pigs were fed respective dietary treatments for 4 weeks. During the last week of the experimental period, pigs were fed their respective dietary treatments containing 0.2% chromic oxide. Fecal samples were collected by rectal palpation daily for the last 3 days after the 4-day adjustment period during the last week of experiment. Blood was collected from randomly selected one pig each pen on d 1, 3, 7, and 14 after weaning. Measurements were growth performance, apparent total tract digestibility (ATTD), TNF-α, TGF-β, CRP, and cortisol by ELISA. Data were analyzed using the MIXED procedure of SAS. The inactivated probiotics increased ADG (471, 501, and 513 vs. 428 g/d; P &lt; 0.05) and G:F (0.65, 0.69, and 0.71 vs. 0.58 g/g; P &lt; 0.05) during overall experimental period compared with CON. The inactivated probiotics increased ATTD of GE (86.87, 87.29, and 88.53 vs. 85.25%; P &lt; 0.05) compared with CON. The inactivated probiotics decreased TNF-α (600, 542, and 523 vs. 849 pg/ml; P &lt; 0.05) and cortisol (5.58, 5.56, and 5.44 vs. 7.25 ng/ml; P &lt; 0.05) on d 7 compared with CON. In conclusion, addition of inactivated probiotics improved growth performance, and nutrient digestibility, and modified immune responses.
PSXI-10 Influence of monensin in the diet of temperate and tropically adapted cattle on the ruminal and fecal metabolome and microbiome
Authors: Rabalais Madeline M , Somenahally Anil C , Wellman Catherine L , Long Charles R , Randel Ronald D 
Published: December 2019

Abstract
The effect of monensin on the ruminal and fecal metabolome and microbiome of temperate and tropically-adapted cattle (264±6 kg BW) consuming Tifton bermudagrass hay plus a supplement was evaluated. During Period 1, 10 Angus, 10 Brahman, and 10 BrahmanXAngus steers were fed Tifton bermudagrass hay ad libitum for a 21-d period with 5 steers per pen within breedtype at the conclusion of which rumen fluid (100-to-250 mL) and fecal samples were collected and stored at -80C. During Period 2 all steers were fed Tifton bermudagrass hay ad libitum plus 1.185 kg of supplement (908 g corn, 217 g soybean meal, 60 g dried molasses) with or without monensin (220 mg·steer−1·d−1) individually for 21 d. Rumen fluid and fecal samples were stored at -80C until DNA was extracted and total prokaryotes, methanogens and fungi determined by qPCR. Rumen and fecal samples were extracted to quantify short chain fatty acids (SCFA) and monensin content by GC and LCMS, respectively. After 21 d of consumption of bermudagrass hay, neither rumen nor fecal prokaryotic copy number, fungal copy number, nor methanogen copy number differed among breedtypes. The rumen, but not fecal, fungi:bacterial ratio was least for Angus (0.77) and similar between Brahman (1.31) and BrahmanXAngus (1.21) steers (P &lt; 0.0032). Ruminal SCFA concentrations were similar among breeds as was the acetate:propionate ratio (Angus: 3.52; Brahman: 3.64; BrahmanXAngus: 3.43). Monensin was not detected in control rumen fluid but averaged 363±24 ng/mL for monensin steers. Monensin reduced the acetate:propionate ratio (P &lt; 0.001) by 18, 25.1, and 18.4% for the Angus, Brahman, and BrahmanXAngus steers, respectively. Rumen prokaryotic copy number (P &lt; 0.10), methanogen copy number (P &lt; 0.035), and methanogen:prokaryote ratio (P &lt; 0.001) were reduced within each breedtype by monensin. Addition of monensin reduced methanogen copy number and improved utilization of the diet in temperate and tropically-adapted beef cattle.
PSXI-28 Reducing dietary crude protein and supplementation with amino acids helps mitigate the environmental impacts of growing pigs production determined by the life cycle assessment
Authors: Esteves Lucas Antonio C , Sitanaka Natália Y , Monteiro Alessandra N T R , Einsfeld Suelen M , Bonagurio Lucas P , Pozza Paulo Cesar 
Published: December 2019

Abstract
This study aimed to evaluate, through life cycle assessment and using experimental data, the environmental impacts of growing pigs’ production (30 to 50 kg) fed diets with reduced crude protein (CP) levels, and supplemented with industrial amino acids. Experiment I (nitrogen and phosphorus balance): 20 crossbred barrows, averaging 31.80 ± 2.39 kg of initial body weight, were housed in metabolic cages and allotted in a randomized block design with four treatments, five replicates and one animal per experimental unit. Experiment II (performance): 44 crossbred barrows, averaging 30.10 ± 0.63 kg of initial body weight, were housed in a randomized block design, with four treatments, 11 replicates and one animal per experimental unit. The treatments for both experiments consisted of four diets containing 18.15, 17.15, 16.15 and 15.15% of CP, and supplemented with industrial amino acids, in order to meet the pig’s requirements of SID amino acids. According to the obtained data (Experiments I and II), the environmental impacts for global warming potential (GWP), acidification potential (AC), eutrophication potential (EU), cumulative energy demand (CED), terrestrial ecotoxicity (TE) and land occupation (LO), per kg of body weight gain, were calculated (CML 2001 method). There was a reduction in some studied environmental impacts as the dietary CP reduced from 18.15 to 15.15%, observing a reduction in the AC from 35.34 to 31.58 g SO2-eq (P = 0.015), there was also a reduction in the EU from 11.90 to 10.31 g PO4-eq (P = 0.001), and the LO was reduced from 2.15 to 1.89 m2-year (P = 0.005), respectively. GWP, CED and TE were not affected (P &gt; 0.05) by the dietary CP reduction, although some variations were observed. It was concluded that the dietary CP reduction, supplementing with industrial amino acids, may help to mitigate the environmental impacts caused by growing pig production for the AC, EU and LO categories.
PSXI-27 Association of an increased marbling score by dietary glycerol supplementation with lipid metabolism gene expression in Korean cattle steers
Authors: Yoo Seon Pil , Fassah Dilla , Baik Myunggi , Na sang Weon , Jeong Inhyuk , Jung Da Jin Sol , Seok-Hyeon Beak , Kang Hyeok Joong , Kim Hyun Jin 
Published: December 2019

Abstract
This study investigated effects of dietary glycerol supplementation on liver, muscle, and adipose gene expression related with gluconeogenesis and lipid metabolism and association of gene expression levels with marbling score in Korean cattle steers. Fourteen Korean cattle steers (average age 28.4 months; average body weight 733 kg) were equally assigned to two groups (0 and 5% glycerol supplementation). Glycerol was provided with glycerol (63%)-adsorbed ground wheat bran (37%, DM) by top dressing during roughage feeding. A concentrate (1.2% of body weight) and 1.0 kg of ryegrass were individually fed twice daily. After four months of study, steers were slaughtered, and marbling score was evaluated. Longissimus thoracis (LT) and subcutaneous adipose tissue at the 13th thoracic vertebra area and liver were collected and analyzed for mRNA levels by quantitative real-time PCR. Statistical significance was analyzed by analysis of variance. Correlations were analyzed using Pearson’s correlation analysis. Glycerol supplementation increased (P = 0.01) marbling score. In the LT, glycerol supplementation tended to increase (0.05 &lt; P ≤ 0.10) lipid uptake CD36 and lipoprotein lipase (LPL) mRNA levels. In subcutaneous adipose tissues, glycerol supplementation increased (P ≤ 0.05) LPL, adipogenic sterol regulatory element binding protein 1 (SREBP1), and lipogenic acetyl CoA carboxylase (ACC) mRNA levels and tended to increase (0.05 &lt; P &lt; 0.10) CD36, adipogenic peroxisome proliferator-activated gamma (PPARG), and lipogenic fatty acid synthase (FASN) expression. It did not affect (P &gt; 0.05) mRNA levels of hepatic gluconeogenesis genes. Marbling score showed significant positive correlations (0.57 &lt; r &lt; 0.68; P &lt; 0.05) with mRNA levels of several genes including LPL, PPARG, SREBP1, and ACC in adipose tissues, but not with any genes examined in the LT. Our study demonstrates that lipid uptake, adipogenesis and lipogenesis may mainly contribute to the increased marbling score by glycerol supplementation.
PSXI-32 Potential improvements in milk production of mid lactating Holstein cows through changes in the defoliation intensity on a mixed pasture
Authors: Hinz Oliver Fast , Chilibroste Pablo , Menegazzi Gabriel , Oborsky Matías , Soca Pablo , Genro Cristina , Mattiauda Diego A 
Published: December 2019

Abstract
An experiment was performed to study the effect of two contrasting defoliation intensities of a fescue based pasture on milk production of 24 mid lactating multiparous cows. The animals were blocked according to parity, body weight (587 ± 42 kg), body condition score (2.77 ± 0.16) and calving date, and were randomly allocated to one of these two treatments: lax treatment (TL) 12 cm and control treatment (TC) 6 cm, with four replications. It was used a second-year pasture of Festuca arundinacea and Lotus corniculatus (2530 ± 180 kgDM/ha), divided into eight plots of 0.3 ha. Each plot was grazed by three cows during as many days as necessary until the defoliation height of each treatment was achieved. This would be determined by daily measures of the pasture height. Animals had one grazing session from 8:00 to 16:00 h. Both treatments received 7.5 kgDM of supplementation (17% Crude Protein; 2.81 Mcal/kgDM) in individual troughs. The cows were milked twice a day (05:00 and 17:00 h). Milk production was registered daily while milk composition was measured at the beginning and at the end of the experiment (samples from two consecutive milking). For milk yield results, a mixed model was used (Glimmix procedure, SAS 9.2, 2010) to analyse with treatment, day and their interaction as fixed effects and block as a random effect. While milk composition was analysed with only treatment as fixed effect. Milk yield was greater for TL than TC, while there was no difference in milk composition between treatments (Table 1). There is a significant effect of the day and an interaction between treatment and day (Figure 1), as difference was greater for TL to the end of the grazing period. This leads to the conclusion that it is possible to improve the milk production through less defoliation intensity.
PSIX-11 Impact of a phytogenic feed additive on growth performance, feed intake and carcass traits of finishing steers
Authors: Brand Tassilo , Hünerberg Martin , McAllister Tim , Saleem Atef , Shen YiZhao , Miller Bryan , Yang Wenzhu 
Published: December 2019

Abstract
The purpose of this study was to evaluate the effect of a phytogenic feed additive (Digestarom® (DA), Biomin, Getzersdorf, Austria) on growth performance, feed intake, carcass traits, and liver abscesses of finishing beef steers. One hundred and twenty Angus × Charolais crossbred steers (initial BW 488 ± 26.5 kg) were used in a 110-d feeding experiment. Steers were blocked by weight and randomly assigned to 12 pens with 10 steers per pen. Each pen was allocated to 1 of 3 diets. Each diet contained 86.5% barley, 10.0% barley silage and 3.5% vitamin and mineral supplement on a dry matter basis. Digestarom® replaced barley grain in the vitamin and mineral supplement at 1.41 and 2.82 g/kg, to achieve average daily intakes of DA of 0 (Control), 0.5 (LowDA) and 1.0 g (HighDA) per steer. Diets were prepared once daily and offered ad libitum intake. Two pens per treatment were equipped to record individual feed intake ehavior. Steers were weighed every 28 d and carcass traits and liver scores were recorded at slaughter. Dry matter intake (average 9.34 kg/d) and final BW (average 669 kg) did not differ among treatments. Average daily gain (ADG) tended (P &lt; 0.09) to increase in response to DA supplementation (control, 1.82; LowDA, 1.87; and HighDA, 1.95 kg/d), but gain:feed ratio was not affected. Supplementation of DA affected rib-eye area quadratically (P &lt; 0.05) with the largest area observed for LowDA. Total abscessed livers were not affected, whereas proportion of severe liver abscesses was numerically lower (P &lt; 0.15) with DA (31 and 43% for LowDA and HighDA, respectively) compared to the control (50%). The positive effect of DA on ADG and percentage of severely abscessed livers should be validated in larger scale feedlot studies.
PSXII-26 Withdrawing monensin by the end of finishing period increases intake and changes ruminal fermentation patterns of cannulated Nellore cattle
Authors: Dellaqua João Victor T , Rigueiro André Luiz N , Silvestre Antonio M , Silva Leandro Aparecido F , Souza Katia Lirian R , Costa Victor Carvalho M , Millen Danilo D 
Published: December 2019

Abstract
This study, conducted at São Paulo State University feedlot, Dracena, Brazil, was designed to evaluate the effects of increasing diet energy content and withdrawing monensin (MON) by the end of the finishing period, while combined with virginiamycin (VM), on rumen fermentation patterns and DMI of Nellore cattle. The experiment was designed as a 5 x 5 Latin square, in which five 32-mo-old Nellore steers (531 ± 22 kg) cannulated in the rumen were utilized. Each period lasted 28-d, which was divided into phases as follows: 14-d adaptation (66% to 78% concentrate), 7-d finishing 1 (84% concentrate) and 7-d finishing 2 (88% concentrate). The treatments were: MON throughout phases, VM throughout phases, MON+VM throughout phases, MON+VM during adaptation and only VM during finishing 1 and 2, and MON+VM during adaptation and finishing 1 and only VM during finishing 2. Monensin and VM were added into diets at 30 and 25 ppm, respectively (DM basis). Rumen fermentation pattern was assessed by measuring ruminal pH and by collecting samples for SCFA determination on the last day of each phase. No treatment effect was observed (P &gt; 0.05) for any of the SCFA variables measured, except for acetate:propionate, where cattle fed MON+VM throughout phases had greater ratio than those fed MON throughout phases on finishing 1 (P = 0.02; Table 1). Cattle consuming only VM in the study presented greater DMI throughout phases (P &lt; 0.01), which did not negatively impact rumen pH (Table 2). Feeding MON+VM and withdrawing MON on finishing 2 did not affect SCFA concentrations, but decreased DMI, when compared to cattle fed VM either throughout phases or just during finishing, without affecting rumen pH. Thus, increasing diet energy content and withdrawing MON by the end of finishing period negatively impacted DMI without altering ruminal fermentation pattern.
PSXII-16 High and medium ADG on LH profile in early-weaned heifers
Authors: Biava Janaina S , Ferraz Marcos V C , Ferreira Evandro M , Polizel Daniel M , Oliveira Gabriela B , Nogueira Guilherme D , Pires Alexandre Vaz 
Published: December 2019

Abstract
This study evaluated the effects of ADG on number of pulses, amplitude and concentration of LH in pre-pubertal early-weaned heifers. Twenty ½Angus x ½Nellore were weaned (86±3 d of age and 91±1 kg BW), and allocated in 2×2 factorial design. Heifers were submitted to high (H) and medium (M) ADG in two periods. The first period occurred from 3 to 7 mo and the second from 7 mo to puberty (HH, HM, MH and MM). Diets in the first (CP 19%, NDF 24% and TDN 79%) and second periods (CP 14%, NDF 24% and TDN 78 %) were the same for all treatments, but the amount was controlled to get the ADG. Weekly, heifers were weighed and puberty status assessed by US. Serial blood samples were collected every 15 min for 12h at 6.5, 9.5 and 11.5 mo of age. The data were evaluated using SAS. The ADG was higher in H than M group in the first period (0.99±0.06 kg vs. 0.51±0.04 kg). In the second period, the ADG was 0.81±0.03 (HH), 0.5±0.03 (HM), 1.0±0.04 (MH) and 0.68±0.03 (MM). At 6.5 mo of age, heifers in H had an increase in LH pulses (2.1±0.5) compared with M (0.7±0.5) (period x age interaction P = 0.04). There was no effect of treatments on LH pulses in other ages, and in amplitude of LH pulse. The LH concentration was higher in HH than HM, MH and MM at 9.5 mo (1.72 vs. 1.07, 1.46 and 1.25±0.2 ng/mL) and 11.5 mo of age (2.01 vs. 1.65, 1.48 and 1.53±0.2 ng/mL), respectively. Despite differences in LH patterns, HH heifers reached puberty at a similar age to HM and MH (12±0.2 mo), however MM heifers (14.4±0.4 mo) reached puberty later (P = 0.09). Increasing ADG on juvenile period increased LH pulses but did not induce precocious puberty.
PSXIV-20 Effects of Saccharomyces cerevisiae CNCM I-1077 on growth efficiency, feeding behavior, physiology, and carcass quality of yearling steers fed a high-grain diet
Authors: Forehand Lydia R , Kayser William , Carstens Gordon E , Chevaux Eric 
Published: December 2019

Abstract
The objective of this study was to evaluate the effects of Levucell SC (LY; Saccharomyces cerevisiae CNCM I-1077) supplementation on performance, feed efficiency, ruminal temperature, feeding behavior, and carcass quality traits in yearling steers. Forty-eight crossbred steers were randomly allocated into two treatments: (1) Control and (2) LY with two pen replicates per treatment (12 steers per pen). Steers were housed in pens equipped with GrowSafe and fitted with reticulo-rumen boluses. All steers were maintained on their respective dietary treatments until harvest to assess carcass traits. There were no differences amongst treatments in DMI, ADG, morbidity or mortality rates, ruminal temperature or F:G. Live-yeast supplemented steers had 17% greater (P = 0.05) backfat thickness than control steers, which resulted in a 13% increase in yield grade, with no differences in all other carcass traits. Live-yeast steers exhibited different feeding behavior patterns than control steers, such that LY steers approached the bunk 25 minutes earlier (P = 0.01) than control steers, had 22% greater (P &lt; 0.05) bunk visit (BV) duration, 41% increased (P &lt; 0.05) head down duration, 18% slower (P &lt; 0.05) BV eating rates, and a tendency for 9% lower BV frequency. Meal criterion was 47% longer (P = 0.01) for LY steers resulting in a reduction (P = 0.07) in meal frequency. Additionally, LY steers consumed 27% longer meals (P &lt; 0.05) that tended to be larger in size (P = 0.09). Although DMI was not different throughout the trial, LY displayed 10% decreased (P &lt; 0.05) meal-eating rates due to longer meal lengths. Overall, LY steers approached the feed bunk sooner following feed delivery, ate fewer, but larger meals at a slower rate, and spent more time eating compared to control steers. Live-yeast supplementation clearly altered feeding behavior patterns, suggesting more favorable fermentation and the capability to mitigate metabolic stress in steers fed high-grain diets by altering meal patterns.
PSXIV-28 Performance of lambs of sheep fed diets containing narasin
Authors: Assis Rhaissa Garcia de , Biava Janaina S , Eckermann Nathalia R , Sturion Thamires U , Souza Terezinha T de , Cerutti Maria Fernanda , Paula Matheus F F V de , Polizel Daniel M , Pires Alexandre Vaz , Ferreira Evandro M 
Published: December 2019

Abstract
The objective of this trial was to evaluate the effects of increasing levels of narasin in the diet of lactating ewes on dry matter intake (DMI) and average daily gain (ADG) of their lambs. Forty crossbred ewes Dorper x Santa Inês with their lambs were assigned to a randomized complete block design (10 blocks and 4 treatments). The treatments were defined by the inclusion of narasin to a base diet (16.8 ± 0.66 of CP) containing 50% of concentrate and 50% of Brachiaria brizanta hay, as follow: 0N - control diet; 13N - 13 mg of narasin/kg of DM; 20N - 20 mg of narasin/kg of DM; 27N - 27 mg of narasin/kg of DM. The experiment lasted from 14 to 70 days of lactation. From the second to the tenth week of age the lambs received initial concentrate (57.0% corn; 25.0% soybean meal; 1.5% limestone; 1.5% of mineral mix; 5.0% of sugarcane molasses and 10% of milk replacer; composition: DM=89.3%; CP = 21.1%; FDN = 8.9%; FDA=2.7%) (ad libitum) in a private feeder. All data were analyzed using the MIXED procedure of SAS. The lambs were weighed weekly after 5-hours fasting. The orts were quantified weekly to determine the DMI. There was no effect of the supply of narasin to the ewes on the DMI by the lambs (0N = 0.126; 13N = 0.088; 20N = 0.153; 27N = 0.145 kg/d; P &gt; 0.05). However, the supply of narasin decreased the ADG (0N = 269.3; 13N = 259.60; 20N = 264.02; 27 = 228.01 g; P &lt; 0.01) of lambs. In conclusion, the use of narasin for the ewes did not benefit lamb performance.
Effect of Different Fruit Developmental Stages and Biochemical Metabolites of Pomegranate (Ponicaceae) on Life History Parameters of Ectomyelois ceratoniae (Lepidoptera: Pyralidae)
Authors: Abedi Zahra , Golizadeh Ali , Soufbaf Mahmoud , Hassanpour Mahdi , Jafari-Nodoushan Ali , Akhavan Hamid-Reza 
Published: December 2019

AbstractPlant–herbivore interactions can be influenced by plant developmental stages. Effects of four different developmental stages of pomegranate fruit (Hazelnut size, Walnut size, Juicy, and Ripe) were investigated on life table parameters, nutritional yield, and energy contents (protein, glycogen, and lipid) of Ectomyelois ceratoniae (Zeller) under controlled conditions in two pomegranate cultivars, namely Shahvare-Danesefid and Esfahani-Daneghermez as susceptible and resistant cultivars, respectively. Biochemical characteristics of the four developmental stages were determined, and a correlation was made with life-history and nutritional responses of E. ceratoniae. Significant differences were observed in developmental time and adult fecundity of E. ceratoniae across various tested stages in both pomegranate cultivars. The highest value of intrinsic rate of increase (rm) was found in Juicy and lowest in Hazelnut-size stage of Shahvare-Danesefid. The highest rm value was on Walnut-size and Juicy stages, and the lower rate was obtained from the feeding of E. ceratoniae on Ripe and Hazelnut-size stages in Esfahani-Daneghermez. A higher relative growth rate (RGR) was observed in Juicy (in Shahvare-Danesefid) and Walnut-size stages (in Esfahani-Daneghermez) when compared with other developmental stages tested. Life-history parameters of E. ceratoniae were correlated with biochemical qualities of pomegranate stages. Results indicated Juicy (Shahvare-Danesefid) and Walnut-size stages (Esfahani-Daneghermez) were relatively susceptible pomegranate stages, and Hazelnut-size and Ripe stages were unsuitable for feeding of E. ceratoniae in Shahvare-Danesefid and Esfahani-Daneghermez, respectively. Findings of this research could aid in the development of integrated management programs of E. ceratoniae in pomegranate orchards.