bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utility of Dehydrated Human Amniotic Membrane (DHAM) in Total Ankle Arthroplasty
Authors: Horn Andrew , Saller Jeremy , Cuttica Daniel J. , Geng Xue , Neufeld Steven 
Published: February 2020

Background: Wound complications after total ankle arthroplasty (TAA) are a common postoperative complication occurring in 14% to 66% of all surgeries. Soft tissue breakdown along the anterior incision can cause exposure of anterior tendons and implant, and adhesions of the extensor tendons of the foot. Recent publications have advocated for the implantation of dehydrated human amniotic membrane (DHAM) allograft during closure of anterior ankle incisions during TAA. The goal of this study was to determine whether implantation of DHAM allograft in TAAs decreased overall postoperative wound complications. Methods: One hundred seventy patients with end-stage ankle arthritis refractory to conservative management underwent TAA with a standard anterior approach by 1 of 3 board-certified foot and ankle orthopedic surgeons. Ninety-one patients underwent closure of the anterior incision with addition of DHAM, whereas 79 patients served as the control (no addition of DHAM). The primary endpoints considered were postoperative complications and reoperation. Included in the postoperative complications was return to the operating room, postoperative plastic surgery intervention, wound communication with the implant, removal of the implant, neurolysis, tendon debridement, and extensor hallucis longus contracture/adhesions. Results: In the analysis of our demographically homogenous cohorts, there was no statistically significant difference in any postoperative complications between patients closed with DHAM and controls. Return to the operating room occurred in 8.9% of controls and 15.4% of the DHAM group ( P = .291). Similarly, there was no statistically significant difference in postoperative plastic surgery, wound communication with the implant, implant removal, neurolysis, and tendon debridement between the control and DHAM groups. Conclusion: The application of DHAM theoretically acts to decrease overall wound complications in TAA. The use of DHAM preceding wound closure in TAA did not show a statistically significant reduction in overall wound complications in our retrospective analysis. Further study, including prospective randomized studies, is needed to further investigate the effectiveness of DHAM in reducing wound complications in TAAs. Level of Evidence: Level III, retrospective cohort study.
Aspirin-Exacerbated Respiratory Disease With Allergic Fungal Rhinosinusitis: A Case Series of Overlapping Sinonasal Endotypes
Authors: Studer Matthew B. , Roland Lauren T. , Ochsner Matthew C. , Cox Daniel , DelGaudio John M. , Wise Sarah K. , Levy Joshua M. 
Published: February 2020

Background Chronic rhinosinusitis with nasal polyposis (CRSwNP) is a heterogeneous inflammatory disease with multiple underlying etiologies. Although several CRSwNP endotypes have been detailed, the current classification system does not adequately represent all patients with nasal polyps. We describe 2 patients meeting established diagnostic criteria for both aspirin-exacerbated respiratory disease (AERD) and allergic fungal rhinosinusitis (AFRS), which are considered distinct CRSwNP endotypes. Objective To describe patients meeting established diagnostic criteria for both AERD and AFRS in order to highlight deficits in the current CRSwNP endotype classification system as well as to expand awareness of the potential for aspirin desensitization as a treatment option in a subset of patients with AFRS. Methods Retrospective chart review with description of patient and diagnostic characteristics. AERD was diagnosed based on the presence of nasal polyps, asthma, and a documented history of sensitivity to nonsteroidal anti-inflammatory drugs. AFRS was diagnosed based on Bent and Kuhn criteria. Results Patients met established diagnostic criteria for both AERD and AFRS. Microscopic examination of sinus tissue revealed reactive bone, tissue eosinophilia (>10/high-powered field), and allergic mucin with noninvasive fungal elements. Subjects had an average of 4 endoscopic sinus surgeries and are currently maintained with adjunctive aspirin desensitization and/or anti-IL-5 biologic therapy. Conclusions This case series suggests that although they are treated as distinct CRSwNP endotypes, AERD and AFRS may have more overlap than was previously appreciated. Further research is needed to accurately identify discrete endotypes and associated treatment outcomes as we seek individualized patient care.
A Comprehensive Coronal and Axial Bone Dimension and Cartilage Thickness Evaluation of the Distal Humerus: Age and Sex Differences
Authors: Misir Abdulhamit , Gunay Ali Eray , Oguzkaya Sinan , Uzun Erdal , Kizkapan Turan Bilge , Yildiz Kadir Ilker , Ozcamdalli Mustafa 
Published: February 2020

Objective There are limited data on bone dimension and cartilage thickness of the distal humeral articular surface. This study aimed to evaluate sex- and age-related bone dimension and cartilage thickness differences and assess the effect of cartilage thickness on distal humeral shape. Design Elbow magnetic resonance images of 180 healthy participants were evaluated. Cartilage thicknesses of the trochlea and capitellum were measured at 19 points using coronal and axial images. In addition, bone diameters were measured from the flexion-extension axis to the 19 points on the coronal and axial magnetic resonance images. Sex differences were evaluated, and the correlation between age and measurement parameters was assessed. Results Significant sex differences regarding the diameters of the axial trochlear bone, coronal lateral trochlear bone, and medial capitellar bone, cartilage thickness at the apex of the lateral trochlear ridge in the axial and coronal plane and at the most lateral point of the capitellar articular surface in the axial plane were observed. A negative correlation was observed between age and axial plane trochlear bone dimensions and between age and coronal plane lateral trochlear and medial capitellar bone dimensions. No significant correlation was found between cartilage thickness and bone dimensions. Conclusions Bone dimension and cartilage thickness at the distal humerus vary according to sex and age. The data could be used in the donor site selection and graft preparation while osteochondral autograft transfer and allograft transplantation, and in the development of gender-compatible hemiarthroplasty implants.
Clinical Outcomes After Medial Patellofemoral Ligament Reconstruction With Suture Fixation of the Gracilis Tendon via Transosseous Tunnels
Authors: Zhang Hangzhou , Ye Mao , Liang Qingwei 
Published: February 2020

Background: Several fixation methods have been introduced in medial patellofemoral ligament (MPFL) reconstruction. However, the optimal management of patients with recurrent patellar dislocation remains controversial. Purpose: To present a case series with a minimum 2-year follow-up of 29 patients with recurrent patellar dislocation who underwent a new transosseous suture fixation technique for MPFL reconstruction. Study Design: Case series; Level of evidence, 4. Methods: From January 2014 through February 2016, a total of 29 patients with recurrent patellar dislocation for which the MPFL was reconstructed with transosseous suture patellar fixation were studied. All patients were available for follow-up (mean, 37.52 months; range, 26-48 months). The patellar attachment was fixed by transosseous patellar sutures. The International Knee Documentation Committee (IKDC) subjective knee score, Kujala score, Tegner score, range of motion, congruence angle, patellar tilt angle, and complications were assessed both pre- and postoperatively. Results: No recurrent dislocation was observed in any of the 29 patients for a minimum of 2 years. All outcome scores improved significantly from preoperatively to postoperatively: the average IKDC subjective knee evaluation score from 53 to 87, Kujala from 54 to 90, Lysholm from 50 to 89, and Tegner from 3 to 5 ( P < .001 for all). The congruence angle significantly decreased from 22° preoperatively to –3° postoperatively, and the patellar tilt angle (Merchant) decreased from 23° preoperatively to 5° postoperatively ( P < .001 for both). In total, 25 patients (25/29; 86.21%) were completely pain-free when performing activities of daily living at the last follow-up, and 27 patients (93.1%) rated themselves as very satisfied or satisfied with the results. Conclusion: In patients with chronic recurrent patellar dislocation, transosseous patellar suture fixation for MPFL reconstruction can significantly improve patellar stability and achieve good results at short-term follow-up.
Clonal tracking in gene therapy patients reveals a diversity of human hematopoietic differentiation programs
Authors: Six Emmanuelle , Guilloux Agathe , Denis Adeline , Lecoules Arnaud , Magnani Alessandra , Vilette Romain , Male Frances , Cagnard Nicolas , Delville Marianne , Magrin Elisa , Caccavelli Laure , Roudaut Cécile , Plantier Clemence , Sobrino Steicy , Gregg John , Nobles Christopher L. , Everett John K. , Hacein-Bey-Abina Salima , Galy Anne , Fischer Alain , Thrasher Adrian J. , André Isabelle , Cavazzana Marina , Bushman Frederic D. 
Published: February 2020

Abstract
In gene therapy with human hematopoietic stem and progenitor cells (HSPCs), each gene-corrected cell and its progeny are marked in a unique way by the integrating vector. This feature enables lineages to be tracked by sampling blood cells and using DNA sequencing to identify the vector integration sites. Here, we studied 5 cell lineages (granulocytes, monocytes, T cells, B cells, and natural killer cells) in patients having undergone HSPC gene therapy for Wiskott-Aldrich syndrome or β hemoglobinopathies. We found that the estimated minimum number of active, repopulating HSPCs (which ranged from 2000 to 50 000) was correlated with the number of HSPCs per kilogram infused. We sought to quantify the lineage output and dynamics of gene-modified clones; this is usually challenging because of sparse sampling of the various cell types during the analytical procedure, contamination during cell isolation, and different levels of vector marking in the various lineages. We therefore measured the residual contamination and corrected our statistical models accordingly to provide a rigorous analysis of the HSPC lineage output. A cluster analysis of the HSPC lineage output highlighted the existence of several stable, distinct differentiation programs, including myeloid-dominant, lymphoid-dominant, and balanced cell subsets. Our study evidenced the heterogeneous nature of the cell lineage output from HSPCs and provided methods for analyzing these complex data.
A T-cell–redirecting bispecific G-protein–coupled receptor class 5 member D x CD3 antibody to treat multiple myeloma
Authors: Pillarisetti Kodandaram , Edavettal Suzanne , Mendonça Mark , Li Yingzhe , Tornetta Mark , Babich Alexander , Majewski Nate , Husovsky Matt , Reeves Dara , Walsh Eileen , Chin Diana , Luistro Leopoldo , Joseph Jocelin , Chu Gerald , Packman Kathryn , Shetty Shoba , Elsayed Yusri , Attar Ricardo , Gaudet François 
Published: February 2020

Abstract
T-cell–mediated approaches have shown promise in myeloma treatment. However, there are currently a limited number of specific myeloma antigens that can be targeted, and multiple myeloma (MM) remains an incurable disease. G-protein–coupled receptor class 5 member D (GPRC5D) is expressed in MM and smoldering MM patient plasma cells. Here, we demonstrate that GPRC5D protein is present on the surface of MM cells and describe JNJ-64407564, a GPRC5DxCD3 bispecific antibody that recruits CD3+ T cells to GPRC5D+ MM cells and induces killing of GPRC5D+ cells. In vitro, JNJ-64407564 induced specific cytotoxicity of GPRC5D+ cells with concomitant T-cell activation and also killed plasma cells in MM patient samples ex vivo. JNJ-64407564 can recruit T cells and induce tumor regression in GPRC5D+ MM murine models, which coincide with T-cell infiltration at the tumor site. This antibody is also able to induce cytotoxicity of patient primary MM cells from bone marrow, which is the natural site of this disease. GPRC5D is a promising surface antigen for MM immunotherapy, and JNJ-64407564 is currently being evaluated in a phase 1 clinical trial in patients with relapsed or refractory MM (NCT03399799).
Mobile health application to support CPAP therapy in obstructive sleep apnoea: design, feasibility and perspectives
Authors: Suarez-Giron Monique , Garmendia Onintza , Lugo Vera , Ruiz Concepción , Salord Neus , Alsina Xavier , Farré Ramón , Montserrat Josep M. , Torres Marta 
Published: February 2020

BackgroundCurrent continuous positive airway pressure (CPAP) devices can be monitored remotely; however, in-person visits are kept for clinical follow-up in order to promote CPAP use and resolve potential side-effects. Mobile health is a promising way to provide remote and easy clinical control for CPAP follow-up and support. We aimed to evaluate the feasibility and acceptance by obstructive sleep apnoea (OSA) patients and healthcare professionals of a newly designed mobile app (Appnea-Q) to promote clinical control through a self-monitoring tool for patients with CPAP supervised by sleep professionals.MethodsAppnea-Q incorporates a simple follow-up questionnaire with automated responses, together with frequent problems and lifestyle recommendations sections. Feasibility, acceptance and usefulness were assessed. First, an internal validation was performed during outpatient CPAP follow-up visits with sleep professionals from various sleep units. Second, an external validation was performed in a subgroup of 15 patients at home.ResultsMost patients (n=75) considered the app useful and were willing to use it and recommend it (72–88%). Up to 64.87% agreed on its capacity to reduce hospital visits. Appnea-Q was rated as acceptable (79.37±19.29) by the system usability score. Sleep professionals (n=30) concurred on its usefulness for OSA patient follow-up, particularly during the first month of CPAP therapy. The external validation showed its feasibility among 11 out of 15 patients and their data were received accordingly on the professionals' web platform.ConclusionsAccording to our validation process, and the viewpoints of the patients and professionals, our new mobile app is a feasible and well-received tool for personal OSA management. Future clinical trials should substantiate its performance and cost-effectiveness in the clinical arena.
Developing a core outcome set for children with protracted bacterial bronchitis
Authors: Gilchrist Francis J. , Ali Imran , Brodlie Malcolm , Carroll Will D. , Kemball Bridget , Walker James , Sinha Ian 
Published: February 2020

BackgroundProtracted bacterial bronchitis (PBB) is a chronic endobrochial infection and a leading cause of chronic wet cough in children. There is an urgent need for a randomised controlled trial to investigate the optimal treatment but there is no core outcome set (COS) to inform choice of outcomes. A COS is a standardised set of outcomes representing the minimum that should be measured and reported in clinical trials of a specific condition. We have developed a COS for PBB.MethodsPotential core outcomes were collated from a systematic review, interviews with parents and a clinician survey. A two-round Delphi survey of healthcare professionals identified which outcomes had consensus for inclusion. The final COS was agreed at a consensus meeting of parent representatives and clinicians.Results20 outcomes were identified for the Delphi survey. After two rounds, 10 reached consensus. These were combined and edited at the consensus meeting into the final six: 1) Resolution of cough assessed using a cough score/diary recorded daily by parent(s) during treatment; 2) relapse of chronic wet cough and/or cumulative antibiotic treatment during ≥12 months follow-up; 3) change in child's quality of life (parent-proxy reporting for young children); 4) emergence of antibiotic resistance; 5) development of bronchiectasis diagnosed on clinically indicated computed tomography scans; and 6) microbiological clearance of identified respiratory pathogen if samples readily available.ConclusionsWe have developed a COS for PBB which will reduce the outcome heterogeneity and bias of future clinical trials, as well as promoting comparison between studies.
Enhanced soil aggregate stability limits colloidal phosphorus loss potentials in agricultural systems
Authors: Li Fayong , Liang Xinqiang , Li Hua , Jin Yingbin , Jin Junwei , He Miaomiao , Klumpp Erwin , Bol Roland 
Published: February 2020

Abstract
Background
Colloid-facilitated phosphorus (P) transport is recognized as an important pathway for the loss of soil P in agricultural systems; however, information regarding soil aggregate-associated colloidal P (Pcoll) is lacking. To elucidate the effects of aggregate size on the potential loss of Pcoll in agricultural systems, soils (0–20 cm depth) from six land-use types were sampled in the Zhejiang Province in the Yangtze River Delta region, China. The aggregate size fractions (2–8 mm, 0.26–2 mm, 0.053–0.26 mm and < 0.053 mm) were separated using the wet sieving method. Colloidal P and other soil parameters in aggregates were analyzed.

Results
Our study demonstrated that 0.26–2 mm small macroaggregates had the highest total P (TP) content. In acidic soils, the highest Pcoll content was observed in the 0.26- to 2-mm-sized aggregates, while the lowest was reported in the < 0.053 mm (silt + clay)-sized particles, the opposite of that revealed in alkaline and neutral soils. Paddy soils contained less Pcoll than other land-use types. The proportion of Pcoll in total dissolved P (TDP) was dominated by < 0.053 mm (silt + clay)-sized particles. Aggregate size strongly influenced the loss potential of Pcoll in paddy soils, where Pcoll contributed up to 83% TDP in the silt + clay-sized particles. The Pcoll content was positively correlated with TP, Al, Fe, and the mean weight diameter. Aggregate-associated total carbon (TC), total nitrogen (TN), C/P, and C/N had significant negative effects on the contribution of Pcoll to potential soil P loss. The Pcoll content of the aggregates was controlled by the aggregate-associated TP and Al content, as well as the soil pH value. The potential loss of Pcoll from aggregates was controlled by its organic matter content.

Conclusion
We concluded that management practices that increase soil aggregate stability or its organic carbon content will limit Pcoll loss in agricultural systems.
A genome-wide scan study identifies a single nucleotide substitution in the tyrosinase gene associated with white coat colour in a red deer (Cervus elaphus) population
Authors: Reiner Gerald , Tramberend Kirsten , Nietfeld Florian , Volmer Klaus , Wurmser Christine , Fries Ruedi , Willems Hermann 
Published: February 2020

Abstract
Background
Red deer with very pale coat colour are observed sporadically. In the red deer (Cervus elaphus) population of Reinhardswald in Germany, about 5% of animals have a white coat colour that is not associated with albinism. In order to facilitate the conservation of the animals, it should be determined whether and to what extent brown animals carry the white gene. For this purpose, samples of one white hind and her brown calf were available for whole genome sequencing to identify the single nucleotide polymorphism(s) responsible for the white phenotype. Subsequently, samples from 194 brown and 11 white animals were genotyped.

Results
Based on a list of colour genes of the International Federation of Pigment Cell Societies, a non-synonymous mutation with exchange of a glycine residue at position 291 of the tyrosinase protein by arginine was identified as the cause of dilution of the coat colour. A gene test led to exactly matching genotypes in all examined animals. The study showed that 14% of the brown animals carry the white gene. This provides a simple and reliable way of conservation for the white animals. However, results could not be transferred to another, unrelated red deer population with white animals. Although no brown animals with a white tyrosinase genotype were detected, the cause for the white colouring in this population was different.

Conclusions
A gene test for the conservation of white red deer is available for the population of the Reinhardswald. While mutations in the tyrosinase are commonly associated with oculocutaneous albinism type 1, the amino acid exchange at position 291 was found to be associated with coat colour dilution in Cervus elaphus.
Comparative genomics applied to Mucor species with different lifestyles
Authors: Lebreton Annie , Corre Erwan , Jany Jean-Luc , Brillet-Guéguen Loraine , Pèrez-Arques Carlos , Garre Victoriano , Monsoor Misharl , Debuchy Robert , Le Meur Christophe , Coton Emmanuel , Barbier Georges , Meslet-Cladière Laurence 
Published: February 2020

Abstract
Background
Despite a growing number of investigations on early diverging fungi, the corresponding lineages have not been as extensively characterized as Ascomycota or Basidiomycota ones. The Mucor genus, pertaining to one of these lineages is not an exception. To this date, a restricted number of Mucor annotated genomes is publicly available and mainly correspond to the reference species, Mucor circinelloides, and to medically relevant species. However, the Mucor genus is composed of a large number of ubiquitous species as well as few species that have been reported to specifically occur in certain habitats. The present study aimed to expand the range of Mucor genomes available and identify potential genomic imprints of adaptation to different environments and lifestyles in the Mucor genus.

Results
In this study, we report four newly sequenced genomes of Mucor isolates collected from non-clinical environments pertaining to species with contrasted lifestyles, namely Mucor fuscus and Mucor lanceolatus, two species used in cheese production (during ripening), Mucor racemosus, a recurrent cheese spoiler sometimes described as an opportunistic animal and human pathogen, and Mucor endophyticus, a plant endophyte. Comparison of these new genomes with those previously available for six Mucor and two Rhizopus (formerly identified as M. racemosus) isolates allowed global structural and functional description such as their TE content, core and species-specific genes and specialized genes. We proposed gene candidates involved in iron metabolism; some of these genes being known to be involved in pathogenicity; and described patterns such as a reduced number of CAZymes in the species used for cheese ripening as well as in the endophytic isolate that might be related to adaptation to different environments and lifestyles within the Mucor genus.

Conclusions
This study extended the descriptive data set for Mucor genomes, pointed out the complexity of obtaining a robust phylogeny even with multiple genes families and allowed identifying contrasting potentially lifestyle-associated gene repertoires. The obtained data will allow investigating further the link between genetic and its biological data, especially in terms of adaptation to a given habitat.