bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of the Comparison of Endoscopic Closure and Laparoscopic Repair of Defect of Gastric Wall
Authors: Qiao Qiao , Tu Huiming , Fei Bojian , Xu Kebin , Yang Fan , Li Jie , Gao Qizhong 
Published: December 2020

Abstract
Objective: To make analysis on the curative effect on the technique of endoscopic closure and laparoscopic closure, and explore the effect and safety of acute perforation of the endoscopic closure technique. Methods: Analysis on the 40 therapeutic cases of laparoscopic repair and endoscopic closure treatments, respectively, to submucosal tumours (SMT) of the stomach removed through ESE/EFR. According to the double-sample t-test, the differences of operation time and medical expenses between the two closure methods were compared and analyzed. And used the chi-square test to compare the difference in terms of operational difficulty and effects. Results: Postoperative pathology of 76 therapeutic cases operated through ESE and EFR was diagnosed as mesenchymal tumor and 4 cases as leiomyoma, among which 34 cases were in fundus ventriculi, 30 in corpus ventriculi and 16 in antrum. Endoscopic closure is significantly lower than laparoscopic closure in operation time span (endoscopic closure VS laparoscopic repair 74.70±23.55min vs 178.35±38.98min, p < 0.001) , medical expenses (endoscopic closure VS laparoscopic repair 28463.55±8228.96rmb vs 61848.75±8812.12rmb, p < 0.001) and inpatient days (endoscopic closure VS laparoscopic repair 10.50±3.49days vs. 16.95±4.58days, P < 0.01), while there is no significant difference in terms of technical difficulty and effects (P > 0.05). Conclusion: With the training and technical progress of endoscopic closure, it is safe, effective and quick in recovery to have non-laparoscopic assisted surgery on gastric wall closure.
WITHDRAWN: Near-infrared Intraoperative Imaging of Pelvic Autonomic Nerves: Clinical Trial
Authors: Jin Hao , Lu Ligong , Cui Min 
Published: December 2020

Abstract
Background: The pelvic autonomic nerves control and regulate anorectal and urogenital function. The dysfunction of pelvic autonomic nerves lead to disorders of anorectum, bladder and male sex organs. Thus the intraoperative identification of pelvic autonomic nerves could be crucial in complications prevention and diseases treatment. Our clinical trial aims at estimating the effectiveness and validity of intraoperative indocyanine green fluorescence imaging in pelvic autonomic nerves identification.Methods: Intraoperative fluorescence imaging using indocyanine green was performed in 10 patients and the feasibility was determined. From February 2019 to June 2019, the 10 patients undergoing laparoscopic colorectectomy was administrated 4.5 mg/Kg indocyanine green 24 hours before surgery. The near-infrared fluorescence imaging was conducted during surgery. A novel white light and near-infrared dual-channel laparoscopic equipment was applied. For each patient, signal background ratio values for pelvic autonomic nerves were recorded and analyzed.Results: We confirmed the best dose and timing of indocyanine green administration was 4.5 mg/Kg and 24 hours before surgery. Using the dual laparoscopic equipment, we could observe the splanchnic plexus, inferior mesenteric artery plexus, and sacral plexus successfully with a high signal background ratio value of 3.18 (standard deviation: 0.48). Conclusion: Pelvic autonomic nerves could be observed using indocyanine green fluorescence imaging during surgery. The novel method may replace the current visual identification method and become the standard clinical practice.
Exploration and Verification of a Six-RNA&nbsp;Binding Proteins-based Prognosis Evaluating Model for Hepatocellular Carcinoma
Authors: Chen Miao , Li Shujie , Zhang Jiakang , Han Jianbo , Wang Lili , Yin Dandan , Yi Changhua , Yi Yongxiang 
Published: December 2020

Abstract
BackgroundRNA binding protein (RBP) plays a crucial role in tumorigenesis at post-transcriptional level in various cancer types. Nevertheless, the role of RBPs in liver hepatocellular carcinoma (LIHC) remains obscure. We attempted to uncover the association between RBPs and the prognosis of LIHC patients. MethodsWe analyzed the transcriptome and corresponding clinical data of LIHC patients from the cancer genome atlas (TCGA) (training cohort) and international cancer genome consortium (ICGC) (validating cohort) database with a series of bioinformatics methods. Differently expressed RNA-binding proteins (DERBPs) were screened and subjected to functional enrichment analysis and co-expression network establishment. Overall survival (OS) related DERBPs and our prognosis risk model were confirmed by univariate, LASSO and multivariate regression analysis in training cohort. Survival analysis, Receiver operating characteristic curve (ROC) and nomogram were conducted in both training and validating groups to confirm the performance of our model. Human protein atlas (HPA) database and Kaplan-Meier plotter were used to verify the expression and prognostic significance of the hub RBPs respectively.Results There were 330 RBPs were found significantly different in TCGA. Functional analysis indicated most of the DERBPs were majored in RNA processing, alternative splicing and metabolism, etc. 6 RBPs (UPF3B, MRPL54, ZC3H13, DHX58, PPARGC1A, EIF2AK4) were recognized as OS related and enrolled into our prognostic model. Survival analysis showed the risk signature was negatively correlated with the OS of LIHC patients in both training (p = 5.808e-06) and validating (p = 3.38e-03) groups. The area under curves (AUC) of the receiver operating characteristics (ROC) curve in training and validating cohorts was 0.756, 0.781 respectively which indicating the good performance of our model. The risk signature was an independent hazardous factor in multivariate COX regression analysis either in TCGA (HR = 1.626;95% CI 1.394 -1.897, p < 0.001) or ICGC (HR = 1.939;95% CI 1.324 -2.838, p < 0.001). Nomogram and calibration curve indicated our model had best performance in predicting 3-year survival rate.ConclusionsWe constructed a six-RBPs based risk signature model which had moderate efficiency in LIHC patients’ prognosis forecasting which may assist practitioners to make better decision in the management of LIHC.
Associated Factories and Time to Occurrence of Tuberculosis Among Children on HIV/AIDS Care in Assosa and Pawe General Hospitals North West Ethiopia: A Retrospective Cohort Study 2020
Authors: Bizuneh Fassikaw kebede , Daba Tadese Tollosa , Mitike Belayneh Mengist , Fikretsion Tamrat sheawno , Negese Belete Negese 
Published: December 2020

Abstract
Background: Tuberculosis (TB) incidence in peadtrics and children living with human immune-deficiency virus (HIV) is an emerging global concern. Although, the incidence of TB among adult HIV patients is exhaustively studied in Ethiopia, but among children on HIV/AIDS care is overlooked. Knowledge of the time when TB develops during successive follow up could be helpful for time relevant intervention strategies.Methods: health institution based retrospective cohort study conducted among 421 children on HIV/AIDS from 2009-2018. Time to develop TB was defined as time from enrollment for ART care until development of TB among children on ART. Proportional hazard assumption was checked for each variable and no variable was found with Schoenfeld test <0.05. Variables with P-value <0.25 at bivariate Cox regression analysis were entered into multivariable Cox model. Multivariable Cox regression model with 95%CI and AHR was used to identify significant predictor variables to develop TB at P< 0.05.Result: Totally 421 children were followed for a total of 662.5 Person Years of observation (PYO). The maximum and minimum follow up time on ART was 0.37 and 4.49 years, respectively. The median age of the children on ART at enrollment was 8 years (IQR=2-15). The Overall incidence density of tuberculosis in HIV infected children was 9.6/ 100 PYOs 95%CI (8.06-10.3). Tuberculosis occurrence among HIV infected children was significantly associated within TB history of contact AHR=3.7, 95%CI (2.89-7.2), not started on cotrimoxazole(CPT) AHR=2.4: 95%CI (1.84-4.74), incomplete vaccination AHR=2.4, 95%CI (1.32-4.5), sever stunting AHR =2.99:95%CI (1.2-7.81), having hemoglobin (Hgb) ≤10 mg/dl AHR = 4.02, 95%CI (2.01-8.1).Conclusion: More than 80% of TB incidences occurred during two years of follow up after ART started. So intensified screening of CPT& therapeutic feeding is highly recommended for all children.
Physiological crosstalk between the Mel1a and Mel1c pathways modulates melatonin-mediated, monochromatic light combination-induced bursa B-lymphocyte proliferation in chickens
Authors: Zhang Yijia , Wang Zixu , Cao Jing , Dong Yulan , Chen Yaoxing 
Published: December 2020

Abstract
Background: Our previous study demonstrated that a combination of green and blue light (G→B or B→G) can improve the antibody titer in chickens compared with single monochromatic light, and melatonin is a critical signaling in the coordination between external light stimulation and the cellular response in the body. This study further clarifies the role of melatonin receptors in monochromatic lights combinations-induced bursa B-lymphocyte proliferation in chickens. Results: A total of 192 chickens were exposed to single monochromatic light (red [R], green [G], blue [B] or white [W] lights) or various monochromatic light combinations (B→G, G→B, R→B) from post-hatching day (P) 0 to P42. With consistency in the upregulation in melatonin level of plasma and melatonin receptors Mel1a, Mel1b and Mel1c expression of bursa, we observed increases in organ index, follicle area, lymphocyte density, B-lymphocyte proliferation, PCNA-positive cells and cyclin D1 expression in bursa of the G→B and B→G group compared with other light-treated groups. Melatonin bound to Mel1a and Mel1c and upregulated p-PKC, p-ERK and p-AKT expression, thereby activating PI3K/AKT and PKC/ERK signaling and inducing B-lymphocyte proliferation in vitro and in vivo. Conclusion: These findings suggested that melatonin modulates a combination of green and blue light-induced B-lymphocyte proliferation in chickens via the Mel1a/PI3K/AKT and Mel1c/PKC/ERK pathways.
Exploring the Caring Incorporating Yoga Program in Promoting Physical Recovery of Stroke Patients in Acute Phase
Authors: Aryal Kalpana Paudel , Hatthakit Urai , Boonyoung Nongnut 
Published: December 2020

Abstract
Background: Caring science integrating cultural practices has become an important health resource to get positive health outcomes for hospitalized acutely ill patients. Yoga, a cultural practice, has the power to bring multiple physical improvements among chronic stroke patients. The purpose of the study is to analyze the experiences of participants by focusing on their roles and perceptions of the power of caring incorporating yoga to promote physical recovery among stroke patients during the acute phase. Methods: The study is part of an action research entitled "Development of a Caring Model Incorporating Yoga for Promoting Physical Recovery and Wisdom of People Living with Stroke" conducted in a neurology unit of a university hospital in Nepal. Purposeful sample was used to select participants consisting of 16 nurses, 16 stroke patients and their family caregivers. Data was collected using semi-structured interview guidelines and was continued until data saturation. An inductive content analysis approach was used for data analysis.Results: Three main themes and nine sub-themes emerged from the qualitative data in terms of experiences of the participants of the program. The three main themes were "caring as a tool to establish trusting relationship in acute phase"; "yoga as a practical and powerful tool for physical recovery from stroke during acute phase"; and "perceived physical recovery as a result of the caring incorporating yoga program". Conclusions: The findings of the study showed that nurses can successfully facilitate early physical recovery of stroke patients using caring incorporating yoga during acute phase. As yoga is well accepted by Hindu stroke patients due to it being culturally congruent with their sociocultural background, it may be effective for physical recovery and healing of the whole person.
Trichoderma asperellum as a promising mycofungicide for managing the dieback disease of tea (Camellia sinensis)
Authors: kumhar kishor , Babu Azariah , Arulmarianathan John Peter , Deka Bhabesh , Bordoloi Mitali , Rajbongshi Hirakjyoti , Dey Pritam 
Published: December 2020

Abstract
Background: The dieback disease of tea caused by Fusarium solani is one of the major constraints in determining its production and quality. Genus Trichoderma is a promising biocontrol agent to control the dieback without any residual effect and is the most suitable option for integrated disease management approach. A few local Trichoderma spp were isolated from tea rhizosphere of the Dooars zone and preliminary identified. Based on dual culture bioassay, the most efficient isolate got re-identified from well reputed national institutes and its liquid formulation (2% Aqueous Suspension) was prepared. Different concentrations of this formulation, T. harzianum and Hexaconazole were evaluated for the control of dieback disease and other parameters for two seasons in three zones under field conditions.Results: The fungal antagonist (KBN-29) was identified as T. asperellum based on characteristics such as regular mycelial branching, typically paired conidiophores with straight phialides and globose to sub-globose shaped conidia. It had the maximum control of dieback pathogen in lab experiment. Plots sprayed with T. asperellum formulation at 1000 and 1200ml/ha concentration conferred better disease control and enhanced the yield of green leaves. The formulation was safe to non-target beneficial insects in all three zones without any phytotoxicity to tea leaves at 4, 8, and 16ml/L concentrations.Conclusions: The present study confirms that T. asperellum 2% AS formulation was significantly superior in managing the dieback disease of tea plantations in Darjeeling, Dooars, and Assam zones at concentration of 1200 followed by 1000 ml / ha during both seasons. The maximum made tea yield was achieved in plots treated with T. asperellum at 1200 followed 1000 ml/ha. This formulation was safe to beneficial insects viz., C. carnea, O. javanus, and S. gilvifrons, furthermore it was not phytotoxic to tea leaves at 4 to 16ml/L concentrations.
XIST: A Meaningful Long Noncoding RNA in NSCLC Process
Authors: Shen Yujie , Lin Yexiang , Liu Kai , Chen Jinlan , Zhong Juanjuan , Gao Yisong , Yuan Chengfu 
Published: December 2020

Background:
A number of studies have proposed that lncRNA XIST plays a role in the development
and chemosensitivity of NSCLC. Besides, XIST may become a potential therapeutic target for NSCLC patients.
The aim of this review is to reveal the biological functions and exact mechanisms of XIST in NSCLC.

Methods:
In this review, relevant researches involving the relationship between XIST and NSCLC are collected
through systematic retrieval of PubMed.

Results:
XIST is an oncogene in NSCLC and is abnormally upregulated in NSCLC tissues. Considerable evidence
has shown that XIST plays a critical role in the proliferation, invasion, migration, apoptosis and chemosensitivity
of NSCLC cells. XIST mainly functions as a ceRNA in the NSCLC process, while XIST also functions
at transcriptional levels.

Conclusion:
LncRNA XIST has the potential to become a novel biomolecular marker of NSCLC and a therapeutic
target for NSCLC.
Remdesivir (GS-5734) in COVID-19 Therapy: The Fourth Chance
Authors: Moirangthem Dinesh S. , Surbala Laishram 
Published: December 2020

Background:
Since its initial start in December 2019 at Wuhan, China, the coronavirus
disease 2019 (COVID-19) has been rapidly spreading and labelled as a pandemic by the World
Health Organization. The rate of human to human transmission of COVID-19 is far higher than severe
acute respiratory syndrome (SARS) and Middle East respiratory syndrome coronavirus
(MERS). With no drugs or vaccines approved for the treatment of the disease, physicians have been
using pre-existing drugs to curb the disease. One potential anti-viral agent currently undergoing
numerous clinical trials is remdesivir, a nucleotide analog that inhibits RNA-dependent RNA polymerase
of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Objective:
In this mini-review, we provide an overview of remdesivir’s journey, mechanism of action,
pharmacokinetics, used in patients with COVID-19 under compassionate use principle and
clinical trials to understand the effect of remdesivir in the treatment of patients with COVID-19.

Conclusion:
Initially, remdesivir was granted an emergency use authorization (EUA) by the U.S.
Food and Drug Administration for the treatment of COVID-19 with severe disease. But now,
remdesivir has been granted for use under EUA to treat all hospitalized COVID-19 patients, irrespective
of their severity of disease.
Screening and Optimization of Protease Enzyme Produced by Strains of Alkalihalobacillus Sp. and Bacillus Sp.
Authors: Saberianpour Shirin , Abkhooie Leila , Elyasifar Babak , Dilmaghani Azita 
Published: December 2020

Background :
Proteases are the most important industrial enzymes with diverse applications
in bacteria, such as Bacillus, commonly used to produce protease for industrial purposes. Proteases
are commercially exploited in a larger scale, especially in pharmaceutical, food, leather, and
detergent industries.

Objective:
The present study sought to screen and optimize protease enzyme activity produced by
local bacteria.

Methods:
The effects of incubation time, temperature, and initial pH were investigated to improve
the extracellular protease enzyme activity by two bacteria, named Bacillus subtilis strain DAR and
Alkalihalobacillus hwajinpoensis strain 3NB. These two isolates have already been isolated and
registered from Iran.

Results:
The results indicated that the optimum incubation time for the protease activity in B. Subtilis
strain DAR is 36 h in contrast to 40 h in Alkalihalobacillus hwajinpoensis strain 3NB. The optimum
incubation temperatures for enzyme activity for B. Subtilis and Alkalihalobacillus hwajinpoensis
are 50°C and 40°C, respectively. Optimum pH values for protease activity of both bacteria
are 8.

Conclusion:
The optimum incubation time, pH, and temperature were investigated for better protease
activity. Further studies are recommended to improve protease activity by changing enzyme
substrates.
Evaluation of a Serious Video Game to Facilitate Conversations About Human Papillomavirus Vaccination for Preteens: Pilot Randomized Controlled Trial
Authors: Cates Joan R , Fuemmeler Bernard F , Stockton Laurie L , Diehl Sandra J , Crandell Jamie L , Coyne-Beasley Tamera 
Published: December 2020

Background
In the United States, the most common sexually transmitted infection, human papillomavirus (HPV), causes genital warts and is associated with an estimated 33,700 newly diagnosed cancer cases annually. HPV vaccination, especially for preteens aged 11 to 12 years, is effective in preventing the acquisition of HPV and HPV-associated cancers. However, as of 2018, completion of the 2- or 3-dose HPV vaccination series increased only from 48.6% to 51.1% in teens aged 13 to 17 years, and this increase was observed only in boys. By comparison, 88.7% of teens had more than one dose of the recommended vaccine against tetanus, diphtheria, and acellular pertussis (Tdap), and 85.1% of teens had more than one dose of meningococcal vaccine. Immunizations for Tdap, meningococcal disease, and HPV can occur at the same clinical visit but often do not.

Objective
Vaccination against HPV is recommended for routine use in those aged 11 to 12 years in the United States, yet it is underutilized. We aimed to develop an educational video game to engage preteens in the decision to vaccinate.

Methods
Land of Secret Gardens is a metaphor for protecting seedlings (body) with a potion (vaccine). We screened 131 dyads of parents and preteens from 18 primary practices in North Carolina who had not initiated HPV vaccination. We measured vaccination intentions, story immersion, and game play and documented HPV vaccination rates. A total of 55 dyads were enrolled, and we randomly assigned 28 (21 completed) to play the game and 27 (26 completed) to the comparison group.

Results
In total, 18 preteens reported playing the game. The vaccination self-efficacy score was higher in the comparison group than the intervention group (1.65 vs 1.45; P=.05). The overall mean decisional balance score trended toward greater support of vaccination, although differences between the groups were not significant.. Vaccine initiation and completion rates were higher in the intervention group (22% vs 15%; P=.31) than in the comparison group (9% vs 2%; P=.10), although the difference was not significant.

Conclusions
Video games help preteens in the decision to pursue HPV vaccination. A serious video game on HPV vaccination is acceptable to parents and preteens and can be played as intended. Gamification is effective in increasing preteen interest in HPV vaccination, as game features support decision making for HPV vaccination.

Trial Registration
ClinicalTrials.gov NCT04627298; https://www.clinicaltrials.gov/ct2/show/NCT04627298