bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Genome-wide Analysis and Expression Profiling of Histone Acetyltransferases and Histone Deacetylases Involved in the Response to Drought in Wheat
Authors: Li Hua , Liu Huajie , Pei Xinxin , Chen Hongyu , Li Xiao , Wang Jiarui , Wang Chenyang 
Published: December 2020

Abstract
Background: Histone acetyltransferases (HATs) and histone deacetylases (HDACs) contribute to plant growth, development, and stress responses. A number of HAT and HDAC genes have been identified in several plants. However, wheat HATs and HDACs have not been comprehensively characterized. In this study, we identified TaHATs and TaHDACs in the wheat genome using bioinformatics tools. Result: In total, 30 TaHAT genes and 53 TaHDAC genes were detected in the wheat genome. As described in other plants, TaHATs were classified into four subfamilies (i.e., GNAT, p300/CBP, MYST, and TAFII250) and TaHDACs were divided into three subfamilies (i.e., RPD3/HDA1, HD2, and SIR2). Phylogenetic and conserved domain analyses showed that TaHATs and TaHDACs are highly similar to those in Arabidopsis and rice; however, divergence and expansion from Arabidopsis and rice were also observed. We detected many stress-related cis-regulatory elements in the promoter regions of these genes (i.e., ABRE, STRE, MYB et al.). Further, based on a comparative expression analyses of three varieties with different degrees of drought resistance under drought stress, we found that TaHAG2, TaHAG3, TaHAC2, TaHDA18, TaHDT1, and TaHDT2 are likely regulate drought stress in wheat. Conclusions: In this study, TaHATs and TaHDACs from the wheat genome were identified. Three TaHATs and three TaHDACs were very likely to regulate drought stress based on a promoter analysis and gene expression analysis. These results provide a foundation for further research on the regulation of acetylation in wheat and its role in the response to drought stress.
A High Burden of Cerebral white Matter Lesion: Evidences from 9 Asian Cities
Authors: Lam Bonnie Yin Ka , Yiu Brian , Ampil Encarnita , Chen Christopher Li-Hsian , Dikot Yustiani , Dominguez Jacqueline , Ganeshbhai Patel Vishal , Hilal Saima , Kandiah Nagaendran , Kim SangYun , Lee Jun-Young , Ong Paulus Anam , Senanarong Vorapun , Leung Kam Tat , Wang Huali , Yang Yuan-Han , Yong Tingting , Arshad Faheem , Alladi Suvarna , Wong Samuel , Ko Ho , Lau Alexander Yuk Lun , Mok Vincent Chung Tong 
Published: December 2020

Abstract
Background Age-related white matter lesion (WML) is considered a manifestation of sporadic cerebral small vessel disease and an important pathological substrate for dementia. Asia is notable for its large population with a looming dementia epidemic. Yet, the burden of WML and its associated risk factors across different Asian societies are unknown. Methods Subjects from 9 Asian cities (Bangkok, Bandung, Beijing, Bengaluru, Hong Kong, Kaohsiung, Manila, Seoul, and Singapore) were recruited (n = 5,701) and classified into i) stroke/transient ischemic attack (TIA), ii) Alzheimer’s disease (AD)/mild cognitive impairment (MCI), or iii) control groups. Data on vascular risk factors and cognitive performance were collected. The severity of WML was visually rated on MRI or CT. ResultsThe prevalence of moderate-to-severe WML was the highest in subjects with stroke/TIA (43.3 %). Bandang Indonesia showed the highest prevalence of WML, adjusted for age, sex, education and disease groups. Hypertension and hyperlipidemia were significant risk factors for WML, and WML was negatively associated with MMSE in all groups. ConclusionsWML is highly prevalent in Asia and is associated with increasing age, hypertension, hyperlipidemia and worse cognitive performance. Concerted efforts to prevent WML will alleviate the huge dementia burden in the rapidly aging Asian societies.
Development and Validation of a Customer Satisfaction Measuring Instrument With Laboratory Services at The University Hospital of Kinshasa
Authors: Alain CHABO , Rostin Mabela Matendo , Joël Konde Numbi , Hippolyte Muhindo , Donatien Kayembe , Koffi Tanon , Jérémie Muwonga , Situakibanza Hippolyte 
Published: December 2020

Abstract
Background: In clinical laboratory, monitoring customers’ satisfaction is an important indicator of the quality management system and required by laboratory quality standards, such as ISO 15189: 2012 and ISO17025: 2017. However, there is no reliable and valid scale to measure clinical laboratory customers’ satisfaction in Democratic Republic of the Congo. In this article, an instrument for measuring customer satisfaction with clinical laboratory services is developed and validated.Methods: In order to develop a reliable and valid measurement tool, the general methodological approach recommended by Churchill was followed. Principal component analysis (PCA) with varimax rotation was used to study the dimensionality of the construct. The developed questionnaire was checked for reliability and validity using exploratory and confirmatory analysis. The reliability checks were done using the internal consistency reliability by analyzing the Cronbach’s Alpha, composite reliability and Jöreskog Rhô values. Confirmatory Factor Analysis (CFA) was used to determine whether the hypothesis of the conceptual framework is acceptable in measuring customer satisfaction.Results: The PCA results showed a three-dimensionality of Customer Satisfaction. Cronbachs alpha coefficients (0.983, 0.981 and 0.981), Jöreskog Rhô (0.973, 0.970 and 0.967) and composite reliability values (0.95, 0.92 and 0.93) of the latent variables were greater than 0.9, which confirms the very high reliability of the model. Indicator loadings were all greater than the threshold of 0.7 or higher. Also, all the latent variables have average variance extracted (AVE) greater than 0.5, therefore, convergent validity has been achieved. Both the Maximum Shared Variance (0.195, 0.297 and 0.234) and the Average Shared Variance (0.805, 0.703 and 0.766) were lower than the AVE (0.897, 0.839 and 0.875) for all the constructs in the scale. Therefore, Discriminant validity has been achieved. Fit indices used to assess CFA and structural equation model were found to be at an acceptable level for the two-factor model where chi-square/df was 1.6, p=0.476, GFI = .99, AGFI= .99, SRMR= .069 , RMSEA= .000 , CFI= 1.00, NFI= .98, RFI= .98, IFI= .98, TLI= .98. Conclusion: The instrument demonstrated acceptable psychometric properties and thus the tool is fit for measuring customer satisfaction with laboratory services.
Person-Centred Quality, Provider Involvement and Family Planning Continuation in India
Authors: Diamond-Smith Nadia , Phillips Beth , Afulani Patience , Srivast Aradhana , Golub Ginger , Sudhinaraset May 
Published: December 2020

Abstract
Background: Quality of care of family planning provision has many dimensions, including those related to a woman’s experience, or person-centered care, and measuring and understanding these different components is challenging. Understanding which components are most important for women’s experiences and method continuation is essential for improving the quality of care provision. The objective of this paper is to explore weather a recently developed scale to measure person-centered family planning experiences is associated with method continuation in India. The secondary objective is to compare this scale to other commonly used measures of quality, and if other measures are more associated with continuation. Methods: We use longitudinal data collected from women in Uttar Pradesh, India (N=225 at baseline, N=179 6 weeks later) to explore the association between different measures of quality (provider preference, provider involvement, and a newly developed scale of person-centered care experiences) and method continuation. Results: We find that a woman’s person-centered care experience is not associated with continuation in this population in India. Providers having a strong method preference is associated with continuation. Conclusion: Certain measures of person-centered quality appear to impact family planning continuation, but the validated person-centered care measure is not. Socio-cultural factors such as power dynamics and gender norms likely impact expectations, and need to be considered in interpreting and choosing quality measures.
Analysis of the Demand for Older Adult Care Services and Related Influencing Factors among Older Adults Living Alone in China
Authors: Zeng Yanbing , Lin Chenxi , Fang Ya 
Published: December 2020

Abstract
Background: The number of older adults living alone in China is rising sharply and their care needs have become an increasing public health concern. This study aimed to explore the anticipated living arrangements and demand for home and community-based care services (HCBS) of older adults living alone and examine the related influencing factors.Methods: The data of 2217 participants living alone were extracted from the 2018 Chinese Longitudinal Health Longevity Survey (CLHLS). A chi-square test was used to examine the differences in older adult care services between respondents with different characteristics. Using the Andersen model as the analysis framework, multinomial and binary logistic regressions were used to analyze the influencing factors of anticipated living arrangements and HCBS, respectively. Results: 75.4% of the respondents hoped to maintain living alone when considering the future. Those who were older, had more children, income source was family members, always felt lonely and isolated and had limitation in activities of daily living (ADL) were inclined to choose the arrangement of “co-residence with children”. Childless and loneliness had significant impact on older adults’ willingness to live in institution. There were significant differences between urban and rural areas for provision of HCBS and the demand exceeds supply in all service types. Those who were younger, lived in urban areas, farmers, income source was family members, had poor economic status, had access to social services, with higher sense of loneliness and chronic diseases tend to show anticipated demands for various HCBS.Conclusions: The demand for older adult care services were affected by predisposing, enabling and need variables. There is a need for an overall improvement of the community care system to create a supportive environment for older adults living alone.
Wide Range Detector of Plasma Induced Charging Effect for Advanced CMOS BEOL Processes
Authors: Chao Yi-Jie , Su Chi , Yang Kai-Wei , Lin Chrong-Jung , King Ya-Chin 
Published: December 2020

Abstract
This work proposed a modified plasma induced charging recorder to widen detection range, for monitoring the possible plasma damage across a wafer during advanced CMOS BEOL processes. New antenna designs for plasma induced damage patterns with extended capacitance are investigated. By adapting the novel PID recorders, maximum charging levels of the detectors have been enhanced.
Genetic diversity of Entamoeba Species among Children Under 5 years in the Vhembe District, Limpopo Province, South Africa
Authors: Maponya Marc , Bessong Pascal , Ngobeni R , Elbakri Ali , Samie Amidou 
Published: December 2020

Abstract
Background: Our understanding of the disease caused by the various Entamoeba species and its epidemiology is changing with time, and very little is known about their genetic diversity. Therefore, this study sought to investigate the prevalence and genetic diversity of Entamoeba species among children under 5 years in the Vhembe District, South Africa.Methods: A total of 534 stool samples from 313 children (both males and females) aged under 5 were collected from 12 villages in Vhembe District, Limpopo. The prevalence of Entamoeba infections was examined by microscope and PCR, followed by Sanger sequencing for specific regions of 18S rRNA gene to identify and differentiate the circulating species of Entamoeba in the study population. Results: Of the 313 children recruited in the study, 163 were females and 150 were males, the ages ranged from 1 to 3 years. Of the 534 samples, 130/534 (24.3%) were microscopically positive for Entamoeba cysts. However, Entamoeba genus-specific DNA ampliﬁcation using PCR identiﬁed 43/534 (8%) of Entamoeba species. Twenty positive amplicons were sequenced by Sanger sequencing technologies. Out of the twenty samples, twelve (60%) were confirmed to be Entamoeba species. The Entamoeba species identified in the study as evidenced by BLAST calls in the NCBI database and phylogenetic tree after narrowing the search using the option Entamoeba taxid (5758) from the NCBI database included 4 E. polecki (33.3%), 6 E. coli (50%), one E. muris (8.3%) and one E. hartmanni (8.3%). The phylogenetic tree showed the close relationship between isolated species and the ones in the GenBank.Conclusion: The current study has shown for the first time the presence of E. polecki in humans and the existence of possibly two types of E. coli infecting humans. Our findings further emphasize the need for the re-evaluation of the pathogenicity of species such as E. polecki which are quite common in the study population and might be responsible for some of the health complications.
Optimum Composition of Developed Additive Based Insect Repellent Paint
Authors: Ahmad Zeeshan , Noor Sana , Ahmad Munir , Rashid Khalid 
Published: December 2020

Abstract
Active compounds of Cymbopogon winterianius, Cymbopogoncitratus, Rosmarinus officinalis, Cedrus, and Eugenol, have been reported to have different insect repellant compounds. Control and development of natural insect repellent additive based decorative coating has driven this work to incorporate the essential oils with long chain fatty acids in water-based lab developed conventional paint. In this work, the Additive based Paint was formulated and its ability to repel different insects was determined. The optimum composition of developed additive based paint was determined by three standard paint analyses which were adhesion, elasticity and insect repellency. As per challenge of Pigment powder and Polymer use for such paint is achieved by testing on Latest instruments. The best composition of additive in paint was found to be 15%. Where it able to repel Pavement Ants, non-biting Flies, Mosquitoes and Black field ant with 80% efficiency. It is envisioned that the formulated paint is effectively function as insect repellent thus as an alternative way to reduce the insect-borne diseases.
Far Infrared Light Irradiation Enhances Microglial Aβ Clearance and Ameliorates Cognitive Deficit in Alzheimer’s Disease-like Mice
Authors: Wang Zhi , Li Qingyong , Wu Wensi , Lu Yanan , Wen Jing , Ren Jiaoyan , Zhao Huiying , Zuo Zhiyi 
Published: December 2020

Abstract
Background: Exposure to sunlight may decrease the risk of developing Alzheimer’s disease (AD). However, the wavelength of the light with this therapeutic effect and the related mechanism remain elusive. Failure to clear amyloid-β (Aβ), the main component of amyloid plaque, has been considered as a key risk to cause the development of AD. As the resident immune cell in the brain, microglia is able to carry out Aβ clearance. We hypothesize that a component of sunlight improves AD-related cognitive dysfunction and that this beneficial effect may be via Aβ clearance by microglia. Method: The APP/PS1 mice by 8.5 months of age were exposed to the visible light (λ = 500 nm), near infrared light (λ = 800 nm) and far infrared light (λ = 3 - 25 µm) for 60 min per day. After 5-week treatment with different light, all mice began to be subjected to Morris water maze behavior test under SPF environment. Western blotting was carried out to detect the expression of postsynaptic density-95 protein and synaptophysin in the hippocampus. The protein amount of interleukin (IL)-1β and IL-6 in the cerebral cortex were determined by using ELISA kits. Immunostaining was performed to characterize the Aβ, microglia, cluster of differentiation 68. Under the condition of Aβ existing, primary cultured microglia were treated with light for 2 h before Aβ phagocytosis assay.Results: The APP/PS1 mice with different light (VIS, NIR and FIR) treatments showed a trend of improvement in learning compared to those without light treatment during training stage. Moreover, FIR light-treated APP/PS1 mice had better spatial memory than APP/PS1 mice in the probe test. Simultaneously, FIR light treatment restored synaptophysin protein expression, promoted the recruitment of microglia to Aβ plaques, enhanced the phagocytosis of Aβ, reduced Aβ burden and alleviated neuroinflammation.Conclusions: Taken together, FIR light treatment ameliorates the learning and memory impairment in AD-like mice. Our findings uncovered a previously unappreciated function of FIR light, suggesting that FIR light treatment may be a potential therapeutic strategy for AD.
Perceived Fear of COVID-19 and its Associated Factors Among Nepalese Older Adults in Eastern Nepal: Findings From a Cross-Sectional Study
Authors: Yadav Uday , Yadav Om Prakash , Singh Devendra Raj , Ghimire Saruna , Rayamajhee Binod , Mistry Sabuj Kanti , Rawal Lal Bahadur , Ali ARM Mehrab , Tamang Man Kumar , Mehta Suresh 
Published: December 2020

Abstract
BackgroundCoronavirus disease 2019 (COVID-19) has impacted all age groups, but older adults may have greater distress given their increased risk for severe illness and mortality. In Nepal, most stories of older adults are untold ̶ both in pre-COVID-19 and the COVID-19 era. In this study, we aimed to assess the perceived fear of COVID-19, and associated factors, among older adults in eastern Nepal.MethodsA cross-sectional study was conducted between July and September 2020 among 847 older adults (≥60 years) residing in three districts of eastern Nepal. Perceived fear of COVID-19 was measured using the seven-item Fear of COVID-19 Scale (FCV-19S). In order to examine the factors associated with the COVID 19 fear, a generalized estimating equation, adjusting the sampling design was used. STATA 15 and JASP 0.13.1 were used for data analyses.ResultsThe mean score of the FCV-19S was 18.1±5.2, and a sizeable proportion of participants, ranging between 12%-34%, agreed to the seven items of the fear scale. Increasing age, Dalit ethnicity remoteness to the health facility, and being concerned or overwhelmed with the COVID-19 were associated with greater fear of COVID-19. Surprisingly, pre-existing health conditions were inversely associated with fear of COVID-19.ConclusionGreater fear of the COVID-19 pandemic among the older population suggests that during unprecedented times such as the current pandemic, the psychological needs of most vulnerable groups should be prioritized. Fear among the most vulnerable groups could be reduced with the flow of adequate genuine information as well as better preparedness and psychosocial interventions.
Development and Validation of Nomograms to Predict Prognosis of Oral and Oropharyngeal Mucoepidermoid Carcinoma: A SEER-Based Study
Authors: liu muyuan , Tong Litian , Xu Manbin , Xu Xiang , Liang Bin , Song Xuhong , Xie Lingzhu , Su Ting , Peng Hanwei , Huang Dongyang 
Published: December 2020

Abstract
Background: Due to the low incidence of mucoepidermoid carcinoma, there lacks sufficient studies for determining optimal treatment and predicting prognosis. The purpose of this study was to develop prognostic nomograms, to predict overall survival and disease-specific survival (DSS) of oral and oropharyngeal mucoepidermoid carcinoma patients, using the National Cancer Institute’s Surveillance, Epidemiology, and End Results (SEER) database. Methods: Clinicopathological and follow-up data of patients diagnosed with oral and oropharyngeal mucoepidermoid carcinoma between 2004 and 2017 were collected from the SEER database. The Kaplan-Meier method with the log-rank test was employed to identify single prognostic factors. Multivariate Cox regression was utilized to identify independent prognostic factors. C-index, area under the ROC curve (AUC) and calibration curves were used to assess performance of the prognostic nomograms. Results: A total of 1230 patients with oral and oropharyngeal mucoepidermoid carcinoma were enrolled in the present study. After multivariate Cox regression analysis, age, sex, tumor subsite, T stage, N stage, M stage, grade and surgery were identified as independent prognostic factors for overall survival. T stage, N stage, M stage, grade and surgery were identified as independent prognostic factors for disease-specific survival. Nomograms were constructed to predict the overall survival and disease-specific survival based on the independent prognostic factors. The fitted nomograms possessed excellent prediction accuracy, with a C-index of 0.899 for OS prediction and 0.893 for DSS prediction. Internal validation by computing the bootstrap calibration plots, using the validation set, indicated excellent performance by the nomograms. Conclusion: The prognostic nomograms developed, based on individual clinicopathological characteristics, in the present study, accurately predicted the overall survival and disease-specific survival of patients with oral and oropharyngeal mucoepidermoid carcinoma.
Rhythm of 24-h Intraocular and Perfusion Pressure in Patients With Ocular Hypertension
Authors: Xia Ran , Shu Na , Cui Huixian , PERDOMO JORGE AGUSTÍN TRUJILLO , Zhang Hong , Chen Zhiqi 
Published: December 2020

Abstract
AIMThe aim of the present study was to characterize the rhythm of 24-h intraocular pressure (IOP) and ocular perfusion pressure (OPP) in patients with ocular hypertension (OHTN), in order to provide a reference for the clinical diagnosis and treatment of OHTN. METHODSAccording to the diagnostic criteria, 107 patients with OHTN were included, and an age- and sex-matched healthy control group (71 patients) was selected. The IOP and blood pressure (BP) of the OHTN and the healthy control groups were recorded every 2 h over a 24-h period. BP was measured using a digital automatic BP monitor, and IOP was measured using a non-contact tonometer. RESULTSThe maximum, minimum and mean IOP were significantly higher in subjects with OHTN than in the healthy control group (P<0.05), and the maximum, minimum and mean MOPP were significantly lower in the OHTN group than in the healthy control group (P<0.05). The minimum and mean SOPP and DOPP values of the OHTN group were lower than those of the healthy control group (P<0.05), and the IOP, MOPP, SOPP and DOPP diurnal and nocturnal fluctuation values were significantly greater in the OHTN group than in the healthy control group (P<0.05). The peak and trough IOP times of the two groups coincided, which tended to be low during the day and higher at night. The peak and trough MOPP and SOPP times of the two groups also coincided, and were primarily higher during the day and lower at night. The 24-h DOPP in the healthy control group was generally higher during the daytime and lower at night, with peak values between 19:00-23:00 h, and trough values between 3:00-7:00 h. No obvious day-to-night fluctuations were observed in the OHTN group. CONCLUSIONThe OPP of patients with OHTN is lower, and the 24-h OPP fluctuates more than that of healthy control subjects. This may be an important blood flow factor for the progression to primary open angle glaucoma in patients with OHTN.