bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal and partner’s level of satisfaction on the delivery room service in University of Gondar Referral Hospital, northwest, Ethiopia: a comparative cross-sectional study
Authors: Taddese Asefa Adimasu , Gashaye Kiros Terefe , Dagne Henok , Andualem Zewudu 
Published: March 2020

Abstract
Background
Asking patients/clients what they perceive about the care and treatment they have received is one of the important steps towards improving the quality of health care. In the scientific world, a number of efforts have been tried to understand about what laboring mothers perceive about the care provided. However, little is known about the birth experiences of partners in Ethiopia. Therefore, this study was aimed to assess the maternal and partner’s level of satisfaction on the delivery room service in the study area.

Methods
A comparative cross-sectional study was conducted from December 2018 to January 2019 in University of Gondar referral hospital. The birth satisfaction scale is used for the mother, and it was adapted to the partners’ perspective. Paired-samples t tests were used for comparing mothers and partners for the birth satisfaction scales global and thematic scores. A binary logistic regression model was fitted to identify predicting factors for mothers’ and partners satisfaction.

Results
The overall satisfaction of mothers in this study was 47.6%. Whereas, 41.2% of partners were satisfied by delivery room services. There were mean difference between mothers and partners’ birth satisfaction scale (p = 0.02). Maternal satisfaction scale was affected by age [OR = 0.36, 95%CI (0.18, 0.73)], perception [OR = 0.02, 95%CI (0.001,0.09)], waiting time [OR = 0.11, 95%CI (0.001, 0.09)],visiting mode [OR = 0.01, 95%CI (0.001,0.08)], pregnancy status [OR = 0.04, 95%CI (0.01,0.33)] and fatal outcome [OR = 0.001, 95%CI (0.001,0.018)] .whereas, partners satisfaction was associated with age [OR = 0.16,95%CI (0.05 0.49)], occupational status [OR = 0.02, 95%CI (0.001, 0.24), amount of money to pay for service [OR = 2.87, 95%CI (1.07, 7.71), visiting mode of his wife [OR = 0.08, 95%CI (0.01, 0.35)], waiting time [OR = 0.12, 95%CI (0.04, 0.33)], privacy [OR = 10.61, 95%CI (3.00, 37.52)], mode of delivery of his wife [OR = 7.69, 95%CI (3.00, 19.69)].

Conclusion
This finding would alert the health care system to design a client-friendly approach. It will provide insight to hospital administrators and providers in formulating a policy that would enhance the support of partners during labour and delivery process.
Identification and engineering of 32 membered antifungal macrolactone notonesomycins
Authors: Goh Falicia , Zhang Mingzi M. , Lim Tian Ru , Low Kia Ngee , Nge Choy Eng , Heng Elena , Yeo Wan Lin , Sirota Fernanda L. , Crasta Sharon , Tan Zann , Ng Veronica , Leong Chung Yan , Zhang Huibin , Lezhava Alexander , Chen Swaine L. , Hoon Shawn S. , Eisenhaber Frank , Eisenhaber Birgit , Kanagasundaram Yoganathan , Wong Fong T. , Ng Siew Bee 
Published: March 2020

AbstractNotonesomycin A is a 32-membered bioactive glycosylated macrolactone known to be produced by Streptomyces aminophilus subsp. notonesogenes 647-AV1 and S. aminophilus DSM 40186. In a high throughput antifungal screening campaign, we identified an alternative notonesomycin A producing strain, Streptomyces sp. A793, and its biosynthetic gene cluster. From this strain, we further characterized a new more potent antifungal non-sulfated analogue, named notonesomycin B. Through CRISPR–Cas9 engineering of the biosynthetic gene cluster, we were able to increase the production yield of notonesomycin B by up to 18-fold as well as generate a strain that exclusively produces this analogue.
Effects of spatial–temporal land cover distribution on gross primary production and net primary production in Schleswig-Holstein, northern Germany
Authors: Ma Liwei 
Published: March 2020

AbstractBackgroundAnnual total Gross Primary Production (GPP) and Net Primary Production (NPP) and the annual total stored GPP and NPP are tightly coupled to land cover distributions because the distinct vegetation conditions of different land cover classes strongly affect GPP and NPP. Spatial and statistical analysis tools using Geographic Information Systems (GIS) were used to investigate the spatial distribution of each land cover class and the GPP and NPP based on the CORINE land cover classification in the federal state, Schleswig-Holstein, Germany for the years 2000, 2006 and 2012.Results“Non-irrigated arable land” and “pastures” were the dominant land cover classes. Because of their large area, “non-irrigated arable land” and “pastures” had higher annual total stored GPP and NPP values than the other land cover classes. Annual total GPP and NPP hotspots were concentrated in the central-western part of Schleswig-Holstein. Cold spots were mainly located in the western and eastern Schleswig-Holstein. The distributions of the annual total GPP and NPP hotspots and cold spots were primarily determined by land cover and land cover changes among the investigated years. The average annual total NPP/GPP ratios were 0.5647, 0.5350 and 0.5573 in the years 2000, 2006 and 2012, respectively. The calculated respiration in 2006 was the highest, followed by those in 2012 and 2000.ConclusionsThe land cover classes with high-ability of carbon stocks in 2000, 2006 and 2012 in Schleswig-Holstein were identified in this study. Furthermore, it is recommendable to enhance the annual total GPP and NPP and the annual total stored GPP and NPP in Schleswig-Holstein by replacing the land cover classes showing low carbon stock capabilities with the classes showing high abilities for the purpose of increasing greenhouse gas fixation.
Integrated analysis of gene expression and DNA methylation profiles in ovarian cancer
Authors: Gong Guanghui , Lin Ting , Yuan Yishu 
Published: March 2020

AbstractBackgroundOvarian cancer is an epithelial malignancy that intrigues people for its poor outcome and lack of efficient treatment, while methylation is an important mechanism that have been recognized in many malignancies. In this study, we attempt to assess abnormally methylated gene markers and pathways in ovarian cancer by integrating three microarray datasets.MethodsThree datasets including expression (GSE26712 and GSE66957) and methylation (GSE81224) datasets were accessed. GEO2R platform was used to detect abnormally methylated-differentially expressed genes. Protein-protein interaction (PPI) networks were built and analysed for hypermethylated and hypermethylated differentially expressed genes using Cytoscape software and Mcode app. GEPIA and cBioPortal platforms were used to validate the expression of the hub genes and the correlation between their mRNA expressions and methylation levels. Kaplan Meier-plotter platform were used to assess the prognostic significance of the hub genes.ResultsSix hundred eighty-one hypomethylated-upregulated genes were detected and involved in Rap1 signaling pathway, biosynthesis of amino acids, endocrine resistance, apoptosis, pathways in cancer. The hub genes were TNF, UBC, SRC, ESR1, CDK1, PECAM1, CXCR4, MUC1, IKBKG. Additionally, 337 hypermethylated-downregulated genes were detected and involved in pathways in cancer, focal adhesion, sphingolipid signaling pathway, EGFR tyrosine kinase inhibitor resistance, cellular senescence. The hub genes were BDNF, CDC42, CD44, PPP2R5C, PTEN, UBB, BMP2, FOXO1, KLHL2. TNF, ESR1, MUC1, CD44, PPP2R5C, PTEN, UBB and FOXO1 showed significant negative correlation between their mRNA expressions and methylation levels. TNF, ESR1 and FOXO1 showed prognostic significance.ConclusionsTwo novel gene networks were found for ovarian cancer. TNF, ESR1, MUC1 and FOXO1 are our candidate genes that might take part in ovarian cancer progression in an epigenetic approach, TNF, ESR1 and FOXO1 may serve as potential markers for ovarian cancer prognosis evaluation.
Effects of low- and high-intensity physical exercise on physical and cognitive function in older persons with dementia: a randomized controlled trial
Authors: Sanders L. M. J. , Hortobágyi T. , Karssemeijer E. G. A. , Van der Zee E. A. , Scherder E. J. A. , van Heuvelen M. J. G. 
Published: March 2020

Abstract
Background
Potential moderators such as exercise intensity or apolipoprotein-E4 (ApoE4) carriership may determine the magnitude of exercise effects on physical and cognitive functions in patients with dementia (PwD). We determined the effects of a 24-week aerobic and strength training program with a low- and high-intensity phase on physical and cognitive function.

Methods
In an assessor-blinded randomized trial, 91 PwD (all-cause dementia, recruited from daycare and residential care facilities, age 82.3 ± 7.0 years, 59 women, Mini-Mental State Examination 20.2 ± 4.4) were allocated to the exercise or control group. In the exercise group, PwD participated in a walking and lower limb strength training program with 12 weeks low- and 12 weeks high-intensity training offered three times/week. Attention-matched control participants performed flexibility exercises and recreational activities. We assessed adherence, compliance, and exercise intensity for each session. We assessed physical (endurance, gait speed, mobility, balance, leg strength) and cognitive (verbal memory, visual memory, executive function, inhibitory control, psychomotor speed) functions with performance-based tests at baseline and after 6, 12, 18, 24, and 36 weeks (follow-up). ApoE4 carriership was determined post-intervention.

Results
Sixty-nine PwD were analyzed. Their mean attendance was ~ 60% during the study period. There were no significant effects of the exercise vs. control intervention on endurance, mobility, balance, and leg strength in favor of the exercise group (Cohen’s d = 0.13–0.18). Gait speed significantly improved with ~ 0.05 m/s after the high-intensity phase for exercise participants (Cohen’s d = 0.41) but declined at follow-up. There were no significant effects of the exercise vs. control intervention on any of the cognitive measures (Cohen’s d ~ − 0.04). ApoE4 carriership did not significantly moderate exercise effects on physical or cognitive function.

Conclusions
Exercise was superior to control activities for gait speed in our sample of PwD. However, the training effect provided no protection for mobility loss after detraining (follow-up). There were no beneficial effects of the exercise vs. control group on cognitive function. Exercise intensity moderated the effects of exercise on gait speed. ApoE4 carriership moderated the effect of exercise on global cognition only (trend level).

Trial registration
Netherlands Trial Register, NTR5035. Registered on 2 March 2015.
Donor information in research and drug evaluation with induced pluripotent stem cells (iPSCs)
Authors: Orzechowski Marcin , Schochow Maximilian , Kühl Michael , Steger Florian 
Published: March 2020

Abstract
Background
The discovery of induced pluripotent stem cells (iPSCs) opened the possibilities for reprogramming cells back to a pluripotent state. Because of no apparent ethical issues connected with donation and derivation of biomaterial, iPSCs are considered as a research alternative to ethically highly disputed human embryonic stem cells (hESCs). However, the unique character of iPSCs leads to numerous ethical considerations, which mainly concern the issue of donor information and consent for the use of biospecimen in research and drug evaluation.

Methods
For the purpose of this analysis, we conducted a review of the literature in the PubMed/MEDLINE and Web of Science databases. The search algorithm led to the identification of 1461 results. After removing duplicates and screening of title and abstract, 90 articles were found to be relevant to the study’s objective. Full texts of these articles were apprised and 62 articles were excluded at this step for not properly addressing the study’s objective. In the final step, 28 articles were included in the analysis. Analyzed were both research and non-research manuscripts published in peer-reviewed journals.

Results
In the case of iPSC research, the information process should be guided by general frameworks established for research on human subjects but also by specific characteristics of iPSCs. We determined four main domains and 12 thematic subdomains that should be included in donor information. Our results show that majority of authors agree to the content of information with regard to the areas of general information, storage of cells, and protection of privacy. Two main issues that are discussed in the literature are donor’s consent for use in future studies and the process of donor information.

Conclusions
Given the unique character of iPSCs and the possibility of their various uses in the future, the content of donor information should contain specific information central to iPSC research. Effective methods of communicating information to donors should combine written and oral information with the possible use of multimedia.
Quantitative γ-H2AX immunofluorescence method for DNA double-strand break analysis in testis and liver after intravenous administration of 111InCl3
Authors: Stenvall Anna , Larsson Erik , Holmqvist Bo , Strand Sven-Erik , Jönsson Bo-Anders 
Published: March 2020

Abstract
Background
It is well known that a severe cell injury after exposure to ionizing radiation is the induction of DNA double-strand breaks (DSBs). After exposure, an early response to DSBs is the phosphorylation of the histone H2AX molecule regions adjacent to the DSBs, referred to as γ-H2AX foci. The γ-H2AX assay after external exposure is a good tool for investigating the link between the absorbed dose and biological effect. However, less is known about DNA DSBs and γ-H2AX foci within the tissue microarchitecture after internal irradiation from radiopharmaceuticals. Therefore, in this study, we aimed to develop and validate a quantitative ex vivo model using γ-H2AX immunofluorescence staining and confocal laser scanning microscopy (CLSM) to investigate its applicability in nuclear medicine dosimetry research. Liver and testis were selected as the organs to study after intravenous administration of 111InCl3.

Results
In this study, we developed and validated a method that combines ex vivo γ-H2AX foci labeling of tissue sections with in vivo systemically irradiated mouse testis and liver tissues. The method includes CLSM imaging for intracellular cell-specific γ-H2AX foci detection and quantification and absorbed dose calculations. After exposure to ionizing radiation from 111InCl3, both hepatocytes and non-hepatocytes within the liver showed an absorbed dose-dependent elevation of γ-H2AX foci, whereas no such correlation was seen for the testis tissue.

Conclusion
It is possible to detect and quantify the radiation-induced γ-H2AX foci within the tissues of organs at risk after internal irradiation. We conclude that our method developed is an appropriate tool to study dose–response relationships in animal organs and human tissue biopsies after internal exposure to radiation.
$L_{1}$-Estimation for covariate-adjusted regression
Authors: Sun Yaodong , Wang Dehui 
Published: March 2020

AbstractWe study a covariate-adjusted regression(CAR) model that is proposed for such situations where both predictors and response in a regression model are not directly observable but are distorted by a multiplicative factor that is determined by an unknown function of some observable covariate. By establishing a connection to varying-coefficient models, we present the local linear$L_{1}$L1-estimation method when the underlying error distribution deviates from a normal distribution. The robust estimators of parameters are proposed in the underlying regression model. The consistency and asymptotic normality of the robust estimators are investigated. Since the limit distribution depends on the unknown components of the errors, an empirical likelihood ratio method based on$L_{1}$L1estimator is proposed. The confidence intervals for the regression coefficients are constructed. Simulation results demonstrate the superiority of the proposed estimators over other classical estimators when the underlying errors have heavy tails. Pima Indian diabetes data set is conducted to illustrate the performance of the proposed method, where the response and predictors are potentially contaminated by body mass index.
New refinement of the Jensen inequality associated to certain functions with applications
Authors: Adil Khan Muhammad , Pečarić Ðilda , Pečarić Josip 
Published: March 2020

AbstractThis article proposes a new refinement of the celebrated Jensen inequality. Some refinements have been obtained for quasi-arithmetic means, Hölder and Hermite–Hadamard inequalities. Several applications are given in information theory. A more general refinement of Jensen inequality is presented associated tonfunctions.
Parameter identification of genetic regulatory network with time-varying delays via adaptive synchronization method
Authors: Liu Chunlin , Wang Fei 
Published: March 2020

AbstractIn this paper, the parameter identification of gene regulatory network with time-varying delay is studied. Firstly, we introduce the differential equation model of gene regulatory network with unknown parameters and time delay. Secondly, for the unknown parameters in the time-varying model, a corresponding system with adaptive parameters and adaptive controller is introduced, and the parameter identification problem of the original model is transformed into the synchronization problem of the two systems. Thirdly, we design an effective adaptive controller and an adaptive law for parameters and construct a Lyapunov functional. Then we give a strict theoretical proof that the adaptive parameters can converge to unknown parameters by Barbalat’s lemma. Finally, a numerical example is given to verify the validity of the theoretical results.
Scalloped hammerhead sharks swim on their side with diel shifts in roll magnitude and periodicity
Authors: Royer Mark , Maloney Kelsey , Meyer Carl , Cardona Edward , Payne Nicholas , Whittingham Kate , Silva Guilherme , Blandino Chloe’ , Holland Kim 
Published: March 2020

AbstractBackgroundGreat hammerhead sharks (Sphyrna mokarran) routinely swim on their sides and periodically roll from side to side. A previous study used wind tunnel tests with a rigid model hammerhead shark to demonstrate that the rolling behavior could improve swimming efficiency using the tall first dorsal fin as a lift-generating surface. Scalloped hammerhead sharks (Sphyrna lewini) also have proportionally taller dorsal fins compared to pectoral fins than most shark species and similar to that of great hammerhead sharks, and thus might exhibit similar rolling behavior. This was assessed by deploying multi-sensor accelerometer instrument packages on free-swimming adult scalloped hammerhead sharks to directly measure swimming depth, body orientation and swimming performance. Specific objectives were to (1) determine whether scalloped hammerhead sharks exhibit side swimming and rolling behavior, (2) characterize the patterns of these behaviors, and (3) evaluate the purpose of these behaviors.ResultsWe obtained 196.7 total days (4720 h) of data from 9 free-swimming adult scalloped hammerhead sharks equipped with multi-instrument biologgers with deployment durations ranging from 7 to 29 days. All sharks exhibited rolling behavior throughout the entire period of observation. The roll angle magnitude and periodicity of rolling showed a clear diel pattern. During daytime, the sharks spent an average of 48% of the time swimming at a roll angle > 30°, with an average roll angle of 41° and rolling periodicity of around 4 min. At night, the sharks spent an average 82% of their time at an angle > 30°, with an average roll angle of 60° and rolling periodicity of around 13 min. In addition to an increase in degree of roll and roll duration, overall dynamic body acceleration (ODBA) also increased at night, and tailbeat frequency was more regular and consistent than during daytime.ConclusionWe observed rolling behavior in scalloped hammerhead sharks similar to that observed in great hammerhead sharks. The diel changes in roll angle and periodicity were accompanied by other changes in swimming behavior. These changes are possibly due to interplay between reducing cost of transport and social interactions with conspecifics.
Cancer Imaging Phenomics via CaPTk: Multi-Institutional Prediction of Progression-Free Survival and Pattern of Recurrence in Glioblastoma
Authors: Fathi Kazerooni Anahita , Akbari Hamed , Shukla Gaurav , Badve Chaitra , Rudie Jeffrey D. , Sako Chiharu , Rathore Saima , Bakas Spyridon , Pati Sarthak , Singh Ashish , Bergman Mark , Ha Sung Min , Kontos Despina , Nasrallah MacLean , Bagley Stephen J. , Lustig Robert A. , O’Rourke Donald M. , Sloan Andrew E. , Barnholtz-Sloan Jill S. , Mohan Suyash , Bilello Michel , Davatzikos Christos 
Published: March 2020

PURPOSE To construct a multi-institutional radiomic model that supports upfront prediction of progression-free survival (PFS) and recurrence pattern (RP) in patients diagnosed with glioblastoma multiforme (GBM) at the time of initial diagnosis. PATIENTS AND METHODS We retrospectively identified data for patients with newly diagnosed GBM from two institutions (institution 1, n = 65; institution 2, n = 15) who underwent gross total resection followed by standard adjuvant chemoradiation therapy, with pathologically confirmed recurrence, sufficient follow-up magnetic resonance imaging (MRI) scans to reliably determine PFS, and available presurgical multiparametric MRI (MP-MRI). The advanced software suite Cancer Imaging Phenomics Toolkit (CaPTk) was leveraged to analyze standard clinical brain MP-MRI scans. A rich set of imaging features was extracted from the MP-MRI scans acquired before the initial resection and was integrated into two distinct imaging signatures for predicting mean shorter or longer PFS and near or distant RP. The predictive signatures for PFS and RP were evaluated on the basis of different classification schemes: single-institutional analysis, multi-institutional analysis with random partitioning of the data into discovery and replication cohorts, and multi-institutional assessment with data from institution 1 as the discovery cohort and data from institution 2 as the replication cohort. RESULTS These predictors achieved cross-validated classification performance (ie, area under the receiver operating characteristic curve) of 0.88 (single-institution analysis) and 0.82 to 0.83 (multi-institution analysis) for prediction of PFS and 0.88 (single-institution analysis) and 0.56 to 0.71 (multi-institution analysis) for prediction of RP. CONCLUSION Imaging signatures of presurgical MP-MRI scans reveal relatively high predictability of time and location of GBM recurrence, subject to the patients receiving standard first-line chemoradiation therapy. Through its graphical user interface, CaPTk offers easy accessibility to advanced computational algorithms for deriving imaging signatures predictive of clinical outcome and could similarly be used for a variety of radiomic and radiogenomic analyses.
Model systems for regeneration: Xenopus
Authors: Phipps Lauren S. , Marshall Lindsey , Dorey Karel , Amaya Enrique 
Published: March 2020

ABSTRACT
Understanding how to promote organ and appendage regeneration is a key goal of regenerative medicine. The frog, Xenopus, can achieve both scar-free healing and tissue regeneration during its larval stages, although it predominantly loses these abilities during metamorphosis and adulthood. This transient regenerative capacity, alongside their close evolutionary relationship with humans, makes Xenopus an attractive model to uncover the mechanisms underlying functional regeneration. Here, we present an overview of Xenopus as a key model organism for regeneration research and highlight how studies of Xenopus have led to new insights into the mechanisms governing regeneration.