bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asian management of hypertension: Current status, home blood pressure, and specific concerns in Indonesia
Authors: Turana Yuda , Tengkawan Jeslyn , Soenarta Arieska Ann 
Published: November 2019

AbstractOver one‐third of the population in Indonesia has hypertension, almost two‐thirds of treated patients have uncontrolled blood pressure (BP), and the majority of patients with hypertension also have comorbidities. Home BP monitoring (HBPM) is a useful tool for diagnosing and managing hypertension. The use of HBPM is recommended by the latest consensus from the Indonesian Society of Hypertension (2019), and nearly, all doctors recommend HBPM for hypertensive patients. However, the use of HBPM in Indonesia is limited by the cost of devices and a perception that these devices are unreliable. In addition, knowledge about proper procedures is lacking. Withdrawal of mercury sphygmomanometers from the end of 2018 is expected to encourage the use of digital sphygmomanometers and the implementation of HBPM. The most common antihypertensive agent used in Indonesia varies by patient age, being angiotensin‐converting enzyme (ACE) inhibitors in those aged 40‐60 years and calcium channel blockers (CCBs) in older patients. Across all age groups, combination therapy with a CCB plus an angiotensin receptor blocker was common (41%‐42% of patients). The high prevalence of hypertension in Indonesia has an important economic impact, and approaches to increase awareness of the disease and adherence to therapy are needed, particularly given data showing the benefits of strict BP control.
Integrative transcriptomic and proteomic analysis reveals CD9/ITGA4/PI3K‐Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma
Authors: Yan Junwei , Yang Xuejiao , Jiao Xuefei , Yang Xian , Guo Mingjin , Chen Yunqing , Zhan Lu , Chen Wenshi 
Published: November 2019

AbstractGlaucoma has been the leading cause of irreversible blindness worldwide. High intraocular pressure (IOP) is a high‐risk factor of glaucoma, repression of which has been the important treatment of glaucoma in clinic. Trabecular meshwork is crucial for maintaining IOP in aqueous humour out‐flow system. It is urgent to reveal the molecular mechanism of trabecular meshwork in glaucoma. Previous studies found that some pathways were related to glaucoma, such as extracellular matrix (ECM)‐receptor interaction, phosphatidylinositol 3‐kinase (PI3K)‐protein kinase B (Akt) and apoptosis. To identify novel molecules in glaucoma, we performed high‐throughput transcriptome and proteome analysis to immortal human trabecular meshwork cells (iHTM) and glaucomatous human trabecular meshwork cells (GTM3), respectively. Twenty‐six up‐regulated genes/proteins and 59 down‐regulated genes/proteins were identified as the high‐risk factors based on differential analysis, including some known factors of glaucoma. Furthermore, a glaucoma‐related protein‐protein interaction (PPI) network was constructed for investigating the function roles of risk factors. Some genes were identified as potential regulator in the pathogenesis of glaucoma based on the topology analysis and module analysis to the network. Importantly, we identified and demonstrated that CD9 played key roles in glaucoma by biological experiment. CD9 is down‐regulated in glaucoma, overexpression of CD9 can active integrin α4 (ITGA4), PI3K and Akt, which lead to the decreased apoptosis and attenuate glaucoma. All these results provide a novel molecular therapy of glaucoma.
Improved Quality of Death and Dying in Care Homes: A Palliative Care Stepped Wedge Randomized Control Trial in Australia
Authors: Liu Wai‐Man , Koerner Jane , Lam Lawrence , Johnston Nikki , Samara Juliane , Chapman Michael , Forbat Liz 
Published: November 2019

OBJECTIVESMortality in care homes is high, but care of dying residents is often suboptimal, and many services do not have easy access to specialist palliative care. This study examined the impact of providing specialist palliative care on residents' quality of death and dying.DESIGNUsing a stepped wedge randomized control trial, care homes were randomly assigned to crossover from control to intervention using a random number generator. Analysis used a generalized linear and latent mixed model. The trial was registered with ANZCTR: ACTRN12617000080325.SETTINGTwelve Australian care homes in Canberra, Australia.PARTICIPANTSA total of 1700 non‐respite residents were reviewed from the 12 participating care homes. Of these residents, 537 died and 471 had complete data for analysis. The trial ran between February 2017 and June 2018.INTERVENTIONPalliative Care Needs Rounds (hereafter Needs Rounds) are monthly hour‐long staff‐only triage meetings to discuss residents at risk of dying without a plan in place. They are chaired by a specialist palliative care clinician and attended by care home staff. A checklist is followed to guide discussions and outcomes, focused on anticipatory planning.MEASUREMENTSThis article reports secondary outcomes of staff perceptions of residents' quality of death and dying, care home staff confidence, and completion of advance care planning documentation. We assessed (1) quality of death and dying, and (2) staff capability of adopting a palliative approach, completion of advance care plans, and medical power of attorney.RESULTSNeeds Rounds are associated with staff perceptions that residents had a better quality of death and dying (P < .01; 95% confidence interval [CI] = 1.83‐12.21), particularly in the 10 facilities that complied with the intervention protocol (P < .01; 95% CI = 6.37‐13.32). Staff self‐reported perceptions of capability increased (P < .01; 95% CI = 2.73‐6.72).CONCLUSIONThe data offer evidence for monthly triage meetings to transform the lives, deaths, and care of older people residing in care homes. J Am Geriatr Soc 68:305–312, 2020
A randomized, patient/evaluator‐blinded, split‐face study to compare the efficacy and safety of polycaprolactone and polynucleotide fillers in the correction of crow's feet: The latest biostimulatory dermal filler for crow's feet
Authors: Jeong Guk Jin , Ahn Ga Ram , Park Su Jung , Hong Ji Yeon , Kim Beom Joon 
Published: November 2019

AbstractBackgroundNo data on the clinical results and safety profiles of the polycaprolactone (PCL) ‐based dermal filler for crow's feet correction have been published.AimsThis study was designed to compare the efficacy and safety of a novel PCL‐based dermal filler, DLMR01, with that of RJR, a purified polynucleotide dermal filler.Patients/MethodsA total of 30 subjects with symmetric crow's feet of 2‐4 points on the Crow's Feet Grading Scale (CFGS) were enrolled in this randomized, patient/evaluator‐blinded, split‐face study. Each subject was randomized to receive injections of DLMR01 or RJR in their right or left crow's feet. At 4 and 12 weeks, all participants were evaluated via CFGS, Global Aesthetic Improvement Scale (GAIS), and PRIMOS software system.ResultsNo significant difference in CFGS, GAIS, and Ra value was detected between DLMR01 side and RJR at 12 weeks (improvement rate in CFGS from baseline at week 12—DLMR01: 48.28% [14/29], RJR: 41.38% [12/29]).ConclusionThe novel PCL‐based dermal filler DLMR01 shows suitable efficacy and safety, widening the selection possibilities for clinicians and patients in the treatment crow's feet.
Phosphorus in the culture of the Amazon river prawn (Macrobrachium amazonicum) and tambaqui (Colossoma macropomum) farmed in monoculture and in integrated multitrophic systems
Authors: Flickinger Dallas L. , Dantas Daniela P. , Proença Danilo C. , David Fernanda S. , Valenti Wagner C. 
Published: November 2019

AbstractInputs, outputs, and accumulation of phosphorus were evaluated for monoculture and polyculture grow‐outs of the Amazon river prawn, Macrobrachium amazonicum and tambaqui, Colossoma macropomum in stagnant earthen ponds using a hypereutrophic water source. A completely randomized experiment was designed with four treatments and three replications: prawn monoculture—monoculture with 30 prawns/m2, fish monoculture—monoculture with 3 fish/m2, integrated multitrophic aquaculture (IMTA)—polyculture with 30 prawns/m2 and 3 fish/m2 reared free swimming, and POLY‐CAGE—polyculture with 30 prawns/m2 and 40 fish/m3 reared in net cages. Samples of commercial diet, water, rain, animals, accumulated sludge, total suspended solids, and settleable solids were collected throughout the experiment to determine the total phosphorus contents of the inputs and outputs and to calculate the phosphorus budget. Results showed high variability in the phosphorus content of each variable in all treatments. Much of the phosphorus was accumulated as solid material at the bottom of the pond (ca. 31–73%). The integrated grow‐outs showed the best phosphorus conversion from the commercial diet (ca. 24–34%) and phosphorus use efficiency from all inputs (ca. 12–18%). Bioturbation from both species appeared to influence the liberation of phosphorus to the water column, stimulating plankton productivity and promoting the turnover of nutrients. A mud‐feeder species should be added to the IMTA systems to take advantage of the large amounts of nutrients in the bottom sediments and increase the uptake of phosphorus by the farmed species.
A detailed mineralogical, petrographic, and geochemical study of the highly reduced chondrite, Acfer 370
Authors: Pratesi Giovanni , Caporali Stefano , Greenwood Richard C. , Moggi Cecchi Vanni , Franchi Ian A. 
Published: November 2019

AbstractAmong the many ungrouped meteorites, Acfer 370, NWA 7135, and El Médano 301—probably along with the chondritic inclusion in Cumberland Falls and ALHA 78113—represent a homogeneous grouplet of strongly reduced forsterite‐rich chondrites characterized by common textural, chemical, mineralogical, and isotopic features. All of these meteorites are much more reduced than OCs, with a low iron content in olivine and low‐Ca pyroxene. In particular, Acfer 370 is a type 4 chondrite that has olivine and low‐Ca pyroxene compositional ranges of Fa 5.2–5.8 and Fs 9.4–33.4, respectively. The dominant phase is low‐Ca pyroxene (36.3 vol%), followed by Fe‐Ni metal (16.3 vol%) and olivine (15.5 vol%); nevertheless, considering the Fe‐oxyhydroxide (due to terrestrial weathering), the original metal content was around 29.6 vol%. Finally, the mean oxygen isotopic composition Δ17O = +0.68‰ along with the occurrence of a silica phase, troilite, Ni‐rich phosphides, chromite, and oldhamite confirms that these ungrouped meteorites have been affected by strong reduction and are different from any other group recognized so far.
The UDP‐GalNAcA biosynthesis genes gna‐gne2 are required to maintain cell envelope integrity and in vivo fitness in multi‐drug resistant Acinetobacter baumannii
Authors: Crépin Sébastien , Ottosen Elizabeth N. , Chandler Courtney E. , Sintsova Anna , Ernst Robert K. , Mobley Harry L. T. 
Published: November 2019

SummaryAcinetobacter baumannii infects a wide range of anatomic sites including the respiratory tract and bloodstream. Despite its clinical importance, little is known about the molecular basis of A. baumannii pathogenesis. We previously identified the UDP‐N‐acetyl‐d‐galactosaminuronic acid (UDP‐GalNAcA) biosynthesis genes, gna‐gne2, as being critical for survival in vivo. Herein, we demonstrate that Gna‐Gne2 are part of a complex network connecting in vivo fitness, cell envelope homeostasis and resistance to antibiotics. The ∆gna‐gne2 mutant exhibits a severe fitness defect during bloodstream infection. Capsule production is abolished in the mutant strain, which is concomitant with its inability to survive in human serum. In addition, the ∆gna‐gne2 mutant was more susceptible to vancomycin and unable to grow on MacConkey plates, indicating an alteration in cell envelope integrity. Analysis of lipid A by mass spectrometry showed that the hexa‐ and hepta‐acylated species were affected in the gna‐gne2 mutant. Finally, the ∆gna‐gne2 mutant was more susceptible to several classes of antibiotics. Together, this study demonstrates the importance of UDP‐GalNAcA in the pathobiology of A. baumannii. By interrupting its biosynthesis, we showed that this molecule plays a critical role in capsule biosynthesis and maintaining the cell envelope homeostasis.
Dynamic Change of Lumbar Structure and Associated Factors: A Retrospective Study
Authors: Wei Jian‐lu , Zhu Yan‐bin , Zhao Da‐wang , Chen Wei , Wang Juan , Wang Hong , Lv Jia‐li , Zhang Tao , Cheng Lei , Zhang Ying‐ze 
Published: November 2019

ObjectiveTo determine whether lumbar anatomy parameters are in dynamic change and related factors.MethodsThis is a retrospective study. Participants who did lumbar computed tomography (CT) scanning in Shandong University Qilu Hospital from October 2017 to March 2019 were selected. The 476 participants were randomly selected as male or female, with the age ranging from 17 to 87 years (mean, 55.19; standard deviation, 14.28 years). All the measurements were taken based on the CT scanning image and the measurement of lumbar morphology was conducted using picture archiving and communication systems (PACS). The angle between the horizontal alignment and pedicle center on median sagittal view, the angle between upper endplate and lower endplate on median sagittal view as well as transverse section angle (TSA) using Magerl point in the axial view was determined by reconstructive CT analysis.ResultsIn the overall participants, the angle between the horizontal alignment and pedicle center on median sagittal view of lumbar one to three was significantly decreased with aging, from 3.90° ± 2.81° to −4.18° ± 6.86° (P = 0.002), 5.60° ± 2.89° to −4.14° ± 5.90° (P = 0.030), and 4.75° ± 2.95° to −2.87° ± 4.68° (P < 0.001), respectively. Additionally, the angle between the horizontal alignment and pedicle center on median sagittal view in male participants of lumbar two was dramatically decreased, from 4.83° ± 2.79° to −4.45° ± 5.97° (P = 0.30). And that of lumbar three in female participants was significantly decreased, from 4.56° ± 2.52° to −2.88° ± 5.03° (P = 0.029). Furthermore, of the overall participants, the angle between upper endplate and lower endplate on median sagittal view of lumbar one to four was associated with aging (P < 0.001, P < 0.001, P = 0.015, P < 0.001, respectively). The angle of lumbar one, two and four in male participants and lumbar one to four in female participants were all significantly related to aging (all P < 0.05). Moreover, in the participants overall, the TSA of lumbar one to three was significantly associated with aging (P = 0.015, P = 0.006 and P = 0.007, respectively). In addition, this angle in lumbar one to lumbar four in male participants were all negatively associated with aging (P = 0.017, P = 0.001, P = 0.005 and P = 0.036, respectively).ConclusionLumbar anatomy parameters are in dynamic change in an age and gender dependent manner. During spine surgery in elderly patients, more attention should be paid to these anatomic changes.
Illness perception of adolescents with allergic conditions under specialist care
Authors: James Polly , Caballero Maria Rosario 
Published: November 2019

AbstractBackgroundOur understanding of how adolescents perceive and cope with their allergic condition/s is limited. This study used the Brief Illness Perception Questionnaire (BIPQ) in a group of adolescents with allergies and correlates the findings with demographic and clinical conditions.MethodsIn this retrospective questionnaire‐based study, we analysed 100 valid questionnaires from adolescents (11‐18 years) attending our service after receiving management and treatment for their allergic condition/s.ResultsThe overall BIPQ score was 4.8 (IQR 3.6‐5.6). The highest item‐related scores were for “timeline” (illness duration) followed by “concern.” The lowest scores were found for “coherence/understanding,” demonstrating that adolescents do not perceive that they have a sound understanding of their condition/s. We found a significant difference in the overall BIPQ scores between the genders and in the emotional representation score, with females showing a highest score. Older adolescents reported a more chronic perception of the allergic disease/s.ConclusionOur results suggest that a better understanding and support of the allergic condition/s in adolescents, particularly in females, are likely an important target for intervention intended to improve their quality of life. BIPQ can be used as adjunct to routine allergy clinic appointments for further exploration of the wider impact of allergies on adolescents’ lives.
Rachis brittleness in a hybrid–parent barley (Hordeum vulgare) breeding germplasm with different combinations at the non‐brittle rachis genes
Authors: Fernández‐Calleja Miriam , Casas Ana M. , Pérez‐Torres Antonio , Gracia Maria Pilar , Igartua Ernesto 
Published: November 2019

AbstractTwo dominant, closely linked and complementary genes, Btr1 and Btr2, control rachis brittleness in barley. Recessive mutations in any of these genes turn the fragile rachis (brittle) into a tough rachis phenotype (non‐brittle). The cross of parents with alternative mutations in the btr genes leads to a brittle F1 hybrid that presents grain retention problems. We evaluated rachis fragility through a mechanical test and under natural conditions, in F1 crosses with different compositions at the btr genes. Brittleness was significantly higher in Btr1btr1Btr2btr2 crosses compared to hybrids and inbred parents carrying one of the mutations (btr1btr1Btr2Btr2/Btr1Btr1btr2btr2). This fact could jeopardize the efficient harvest of hybrids bearing alternative mutations, reducing the choice of possible crosses for hybrid barley breeding and hindering the exploitation of potential heterotic patterns. Furthermore, non‐brittle hybrids showed higher brittleness than inbreds, suggesting the presence of other dominant factors affecting the trait. In conclusion, this work encourages a deeper study of the genetic control of the rachis brittleness trait and urges the consideration of rachis tenacity as a target for hybrid barley breeding.
GNAQQ209L expression initiated in multipotent neural crest cells drives aggressive melanoma of the central nervous system
Authors: Urtatiz Oscar , Cook Courtney , Huang Jenny L.‐Y. , Yeh Iwei , Van Raamsdonk Catherine D. 
Published: November 2019

AbstractPrimary leptomeningeal melanocytic neoplasms represent a spectrum of rare tumors originating from melanocytes of the leptomeninges, which are the inner two membranes that protect the central nervous system. Like other non‐epithelial melanocytic lesions, they bear frequent oncogenic mutations in the heterotrimeric G protein alpha subunits, GNAQ or GNA11. In this study, we used Plp1‐creERT to force the expression of oncogenic GNAQQ209L in the multipotent neural crest cells of the ventro‐medial developmental pathway, beginning prior to melanocyte cell differentiation. We found that this produces leptomeningeal melanocytic neoplasms, including cranial melanocytomas, spinal melanocytomas, and spinal melanomas, in addition to blue nevus‐like lesions in the dermis. GNAQQ209L drove different phenotypes depending upon when during embryogenesis (E9.5, E10.5, or E11.5) it was induced by tamoxifen and which Cre driver (Plp1‐creERT, Tyr‐creERT2, or Mitf‐cre) was used. Given these differences, we propose that melanocytes go through temporary phases where they become sensitive to the oncogenic effects of GNAQQ209L. R26‐fs‐GNAQQ209L; Plp1‐creERT mice will be useful for defining biomarkers for potentially aggressive leptomeningeal melanocytomas and for developing new therapeutics for advanced disease.
Genital vitiligo in children: Factors associated with generalized, non‐segmental vitiligo
Authors: Sink Jacquelyn R. , Silverberg Nanette B. 
Published: November 2019

AbstractBackground/ObjectivesIt remains uncertain as to whether genital vitiligo of childhood is segmental or a forme fruste of non‐segmental disease. This type of vitiligo is sometimes termed figure‐of‐eight disease due to female predominance and perineal and perianal hypopigmentation or depigmentation around two orifices, the anal and vaginal introitus. The objective of this series was to categorize the features of genital‐limited vitiligo of childhood including comorbidities, family history, and long‐term course.MethodsIRB‐approved retrospective chart review of vitiligo cases.ResultsEight cases of vitiligo that began in childhood in the genital area were identified, including clinical course and comorbidities. Involvement of the contiguous skin, including inner thighs, buttocks, and scrotum, was noted in all males. In females, only 1 patient had other sites of vitiligo, which was a solitary lesion on the chest. Therapeutic response to topical corticosteroids for perianal skin was seen in five of six children and topical calcineurin inhibitors with or without calcipotriene in four out of four patients.ConclusionsSegmental disease limited to the genitalia was more common in females than males, suggesting that perhaps intervention and work‐up for comorbid autoimmune diseases may be required for males with genital‐limited vitiligo at onset, but may be deferred in females and added if observation of spread is noted. Differentiation from lichen sclerosus et atrophicus may require biopsy.
Incidence, risk factors, and outcome of blood stream infections during the first 100 days post‐pediatric allogeneic and autologous hematopoietic stem cell transplantations
Authors: Youssef Ahmed , Hafez Hanafy , Madney Youssef , Elanany Mervat , Hassanain Omneya , Lehmann Leslie E. , El Haddad Alaa 
Published: November 2019

AbstractBloodstream infections (BSI) are a frequently observed complication after hematopoietic stem cell transplant (HSCT). Retrospective analysis of clinical and microbiological data during the first 100 days from 302 consecutive pediatric patients who underwent HSCT for a malignant disease at our institute between January 2013 and June 2017. A total of 164 patients underwent autologous and 138 allogeneic HSCT. The overall incidence of BSI was 37% with 92% of infectious episodes occurring during the pre‐engraftment phase. Gram‐positive bacteria (GPB) accounted for 54.6% of the isolated pathogens, gram‐negative bacteria (GNB) for 43.9%, and fungi for 1.4%. Coagulase‐negative staphylococci and Escherichia coli were the most commonly isolated GPB and GNB, respectively. Forty‐five percent of GNB were extended‐spectrum beta‐lactamase producers and 21% were multidrug‐resistant organisms. Fluoroquinolone resistance was 92% and 68%, among GPB and GNB, respectively. Risk factors for BSI in univariate analysis were allogeneic HSCT, delayed time to engraftment more than 12 days, previous BSI before HSCT, and alternative donor. In multivariate analysis, only HSCT type (allogeneic vs autologousP = .03) and previous BSI within 6 months before HSCT (P = .016) were significant. Overall survival at day 100 was 98% and did not differ significantly between patients with and without BSI (P = .76). BSI is common in children undergoing HSCT for malignant diseases. Allogeneic HSCT recipients and previous BSI within 6 months before HSCT are associated with increased risk of post‐transplant BSI. With current supportive measures, BSI does not seem to confer an increased risk for 100‐day mortality.