bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aquaporin 3 promotes human extravillous trophoblast migration and invasion
Authors: Nong Yingqi , Li Shifen , Liu Wenjuan , Zhang Xiqian , Fan Lin , Chen Ye , Huang Qianwen , Zhang Qianyu , Liu Fenghua 
Published: January 2021

Abstract
Problem: Does aquaporin 3 (AQP3) affect the migration and invasion of human extravillous trophoblast (HTR8/Svneo) cells?Method of Study: A lentivirus infection system was used to construct stable cell lines with either AQP3 knockdown or overexpression. RT-PCR and western blotting were used to verify the efficiencies of AQP3 knockdown or overexpression in HTR8/Svneo cells at mRNA and protein levels, respectively. Cell Counting Kit-8 and flow cytometry assays were used to detect the influence of AQP3 knockdown or overexpression on proliferation and apoptosis of HTR8/Svneo cells. In addition, wound healing and Transwell invasion assays were used to detect the effects of AQP3 knockdown or overexpression on migration and invasion capabilities of HTR8/Svneo cells. An Agilent gene chip was used to screen for significant differentially expressed genes after AQP3 knockdown. Finally, mechanisms by which AQP3 influences the migration and invasion of HTR8/Svneo cells were explored using bioinformatic analysis.Results: Compared with controls, migration and invasion capabilities of HTR8/Svneo cells were significantly reduced after AQP3 knockdown, and significantly increased after AQP3 overexpression. Subsequent bioinformatic analysis of gene chip expression profiles indicated downregulation of genes related to adhesion such as PDGF-B, as well as signaling pathways (such as PIK3/AKT, NF-κB, and TNF) after AQP3 knockdown. Conclusions: AQP3 could significantly promote migration and invasion capabilities of human extravillous trophoblasts, it may mediate embryo invasion and adhesion to endometrium by regulating PDGF-B, PIK3/AKT signaling pathways, although this requires further verification.
Efficient production of human growth factors in Escherichia coli by fusing with small protein 6HFh8
Authors: Kim Young Su , Lee Hye-Jeong , Han Man-ho , Yoon Nam-kyung , Kim Yeu-chun , Ahn Jung-Oh 
Published: January 2021

Abstract
Background: Growth factors (GFs) are signaling proteins that affect cellular processes such as growth, proliferation, and differentiation. GFs are used as cosmeceuticals, exerting anti-wrinkle, anti-aging, and whitening effects, and also as pharmaceuticals to treat wounds, growth failure, and oral mucositis. However, in mammalian and bacterial cells, low productivity and expression in inclusion bodies, respectively, of GFs does not satisfy the consumer demand. Here, we aimed to develop a bacterial expression system that produces high yields of soluble GFs that can be purified in their native forms.Results: We present Fh8, an 8-kDa peptide from Fasciola hepatica with an N-terminal hexa-histidine (6HFh8), as a fusion partner for enhanced human GF production in recombinant Escherichia coli. The fusion partner harboring a tobacco etch virus (TEV) protease cleavage site was fused to the N-terminus of 10 human GFs: acidic and basic fibroblast growth factors (aFGF and bFGF, respectively), epidermal growth factor (EGF), human growth hormone (hGH), insulin-like growth factor 1 (IGF-1), vascular endothelial growth factor 165 (VEGF165), keratinocyte growth factor 1 (KGF-1), placental growth factor (PGF), stem cell factor (SCF), and tissue inhibitor of metalloproteinase 1 (TIMP-1). The fusion proteins were expressed in E. coli under the control of T7 promoter at three temperatures (25 °C, 30 °C, and 37 °C). All individual fusion proteins, except for SCF and TIMP-1, were successfully overexpressed in cytoplasmic soluble form at more than one temperature. Further, the original aFGF, IGF-1, EGF, and VEGF165 proteins were cleaved from the fusion partner by TEV protease. Five-liter fed-batch fermentation approaches for the 6HFh8-aFGF (lacking disulfide bonds) and 6HFh8-VEGF165 (a cysteine-rich protein) were devised to obtain the target protein at concentrations of 9.7 g/l and 3.4 g/l, respectively. The two GFs were successfully highly purified (>99% purity). Furthermore, they exerted similar cell proliferative effects as those of their commercial equivalents.Conclusions: We demonstrated that 6HFh8-GF fusion proteins could be overexpressed on a g/l scale in the cytoplasm of E. coli, with the GFs subsequently highly purified and maintaining their biological activity. Hence, the small protein 6HFh8 can be used for efficient mass-production of various GFs.
Generowanie MostkÓW Disiarczkowych W BiaŁKach – RÓŻNorodnoŚĆ Strukturalna I Funkcjonalna BiaŁEk Dsba
Authors: Banaś Anna Marta , Petrykowska Anna , Jagusztyn-Krynicka Elżbieta Katarzyna 
Published: January 2021

Abstract
Bacterial proteins of the Dsb (disulfide bond) system catalyze the formation of disulfide bridges, a post-translational modification of extra-cytoplasmic proteins, which leads to stabilization of their tertiary and quaternary structures and often influences their activity. DsbA – Escherichia coli monomeric oxidoreductase is the best studied protein involved in this process. Recent rapid advances in global analysis of bacteria have thrown light on the enormous diversity among bacterial Dsb systems. The set of Dsb proteins involved in the oxidative pathway, varies, depending on the microorganism. In this article we have focused on characterization of structural and phylogenetic groups of monomeric DsbAs. This review discuss their physicochemical features and interactions with redox partners as well as with substrate proteins. The last part of the review concentrates on dimeric oxidoreductases responsible for disulfide generation. Many virulence factors are the substrates of the Dsb proteins. Thus unraveling the machinery that introduces disulfide bonds and expanding knowledge about Dsb protein structures and their activities may facilitate the discovery of an effective anti-bacterial drugs.
1. Introduction. 2. Escherichia coli Dsb system. 2.1. Characteristic of the E. coli thiol oxidoreductase – DsbA. 2.2. Izomerization / reduction pathway proteins. 3. Classification of the monomeric DsbAs. 3.1. Physicochemical features of different classes of DsbAs. 4. DsbA interactions with redox partner and substrates. 4.1. DsbA interactions with redox partner. 4.2. DsbA interactions with substrates. 5. Dimeric Dsb proteins with oxidative activity. 6. Conclusions. 7. References
Bacillus Thuringiensis – Nowy PotencjaŁ Aplikacyjny
Authors: Gęsicka Aleksandra , Henschke Agata , Barańska Zuzanna , Wolna-Maruwka Agnieszka 
Published: January 2021

Abstract
One of essential bacteria used in modern agriculture, in particular because of its ability to eradicate insects, is Bacillus thuringiensis. Cry and Cyt proteins produced by Bt are selective, therefore using those proteins eliminates only larvae of target insects. There are various other known substances produced by Bt bacteria, that may help with further elimination of pests and promoting plant growth. Furthermore, there are attempts being made to use Bt strains in bioremediation of contaminated sites as well as in medicine, especially in combating human and animal pathogens, or cancer cells.
1. Introduction. 2. Characteristics of Bacillus thuringiensis. 3. Virulence factors of Bacillus thuringiensis. 4. Applications of Bacillus thuringiensis in modern agriculture 5. Novel possible applications of Bacillus thuringiensis. 6. Conclusions
Enhanced removal of Cr(VI) using a modified environment-friendly adsorbent
Authors: Liu Shan , Cao Lei , Tian Xincheng , Li Xuanni , Liao Lei , Zhao Chunpeng 
Published: January 2021

Abstract
Chitosan (CS) was modified with ferric chloride hexahydrate (FeCl3·6H2O) and sodium laurylsulfonate (SLS) to prepare the compound adsorbent CS-Fe-SLS. Taking Cr(VI) as the target pollutant, the influence of different factors (solution pH, adsorption time, initial adsorption concentration and coexisting ions) were investigated. The results showed that the optimal pH value was 3.0; the adsorption equilibrium was reached at 120 min, with both physical and chemical process being involved in the adsorption process; the maximum adsorption value was 131.91 mg/g, with the adsorption being both single-layer and multi-layer adsorption; Cl−, and had little effect on adsorption, reduced it and Cu(II) increased it. The characterization results of scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction showed that CS-Fe-SLS and chitosan had different morphological characteristics: in contrast with chitosan, CS-Fe-SLS had a larger volume and more flakes on the surface. CS-Fe-SLS was significantly lower in crystallinity than chitosan, and was more irregular and amorphous in shape, and the experimental results showed that CS-Fe-SLS had better adsorption capacity for Cr(VI). The adsorption Cr(VI) was related to -NH and -OH on the material. Some Cr(VI) was reduced to Cr(III) during the adsorption process.
Benefits of using Blockchain technology as an accounting auditing instrument
Authors: Maervelym Pâmella de Andrade Simões  , Janeide Albuquerque Cavalcanti  , Janaina Ferreira Marques de Melo  , Cristiane Queiroz Reis  
Published: January 2021

Purpose: Analyze the possible benefits of using Blockchain technology as a tool for accounting auditing.
Methodology: In order to achieve the objective of this study, a theoretical essay was conducted through a selection of national and international literature on accounting auditing and the potential benefits of Blockchain technology, analyzing the relationship of these potential benefits with the procedures adopted by the accounting auditor.
Results: Findings from the literature show that despite the complexities, Blockchain technology offers an opportunity to improve financial reporting and audit processes. Adoption of this technology may allow auditors to develop procedures to obtain audit evidence directly from Blockchains and to adapt procedures to reap its benefits, as well as address incremental risks and that while the audit process may become more continuous, auditors may They will still have to apply professional judgment in analyzing accounting estimates and other judgments made by management in preparing the financial statements.
Contributions of the Study: It is believed that this work contributes to an active and continuous dialogue regarding the use of Blockchain in audit processes, as well as the knowledge of advantages that this technology offers to professionals, as well as arouse the interest of research on the use of Blockchain technology in accounting auditing.
Institutionalization of management accounting rules and routines in a private educational foundation in Santa Catarina: institutional approach of the Brazilian third sector
Authors: Renícia Maria Innocenti  , Rogério João Lunkes  , Valdirene Gasparetto  
Published: January 2021

Purpose: To verify the change of rules and routines of management accounting of a third sector organization in the light of the Old Institutional Economy (OIE).
Methodology: To analyze the institutional change in management accounting, documentary, descriptive and qualitative research was carried out. The research was carried out at a private educational foundation in Santa Catarina, selected for being a member of the third sector and for its accessibility. Data collection took place through interviews and documents, between the months of September 2018 and February 2019. For data analysis, qualitative content analysis was used.
Results: In the institutional approach, the change in management accounting for the Foundation was classified as formal, revolutionary and progressive. The institutionalization of management accounting rules and routines was promoted by the need for management to have more agile and secure tools to support decision making. The rules and routines of management accounting have been modified and / or abandoned, over time, with the reconfiguration of existing practices, for their innovation. The integration of the Foundation’s systems based on the standardization and sharing of accounting and tax information has provided an improvement in the quality of information, better integration with the tax authorities and faster access to information, giving rise to more efficient, safe and useful management controls.
Contributions of the Study: The research contributes to the accounting literature by presenting empirical evidence on the process of institutionalization of management accounting over time, in Brazilian third sector organizations, suggesting new studies. As a practical contribution, there is the possibility of improving the management models of third sector organizations through the understanding of the dynamics used in the process of change in management accounting, by the agents involved.
The Cytotoxic and Immunomodulatory Effects of Titanium Dioxide Nanoparticles and Sargassum oligocystum on Toxoplasma gondii In Vitro and In Vivo
Authors: Asadi Negar , Khademvatan Shahram , Hajipirloo Habib Mohammadzadeh , Heshmatiyan Behnam , Asghari Sadegh , Foroutan Masoud 
Published: January 2021

Objective:
This study was undertaken to evaluate the effect of titanium dioxide (TiO2)
nanoparticles (NPs) and methanolic extract of Persian Gulf brown algae (Sargassum oligocystum)
on the growth and cell death of T. gondii tachyzoites in vitro and in vivo.

Methods:
Six- to eight-week-old female BALB/c mice (n = 28) were used for the treatment experiment
and infected with 105 T. gondii tachyzoites. Four days after treatment, IFN-γ and the levels of
splenic lymphocyte proliferation were measured. All the groups were challenged with T. gondii,
and the survival rate of experimental mice was assessed. The effects of TiO2NPs and S. oligocystum
on the proliferation of T. gondii were evaluated by MTT and annexin V staining in vitro.

Results:
Based on the results, the combination of S. oligocystum extract and TiO2NPs exhibited a
more cytotoxic effect compared to their use separately. The results of the challenge test also revealed
that mice which received combined treatment had the highest life time expectancy than
those receiving the treatment alone.

Conclusion:
The simultaneous use of immunomodulatory compounds for the stimulation of the immune system as well as
S. oligocystum and TiO2NPs with antiparasitic activity can be promising to develop an effective drug for the treatment of
toxoplasmos.
Avaliando entidades mencionadas na coleção ELTeC-por
Authors: Santos Diana , Bick Eckhard , Wlodek Marcin 
Published: January 2021

Este artigo relata a preparação da anotação da coleção ELTeC-por com entidades mencionadas apropriadas ao género textual "romances e novelas publicadas entre 1840 e 1920", para possibilitar a leitura distante em português.
Em primeiro lugar apresentamos a coleção ELTeC-por, compilada no âmbito da ação COST "Distant Reading for European Literary History" para estudar a literatura europeia, e explicamos as diversas restrições e escolhas necessárias, fornecendo uma caracterização inicial segundo vários eixos: a origem e tamanho das obras, o seu (sub)género literário, o género do autor, o local de publicação e a existência ou não de mais edições.
Em seguida apresentamos o sistema PALAVRAS-NER, com o qual anotaremos a coleção, explicando detalhadamente o seu funcionamento.
Passamos então à descrição da criação de uma subcoleção de oito obras revistas, que servem, por um lado, para avaliar o desempenho do sistema de REM automático, e, por outro, para caracterizar o tipo de população esperada. As obras podem classificar-se segundo dois eixos diferentes: romances históricos vs. romances contemporâneos; e obras com grafia original ou grafia modernizada. Além disso, algumas obras são obviamente canónicas, outras não.
Além da descrição quantitativa do resultado de anotação e revisão, apresentamos algumas considerações qualitativas sobre o processo.
Também fornecemos uma análise detalhada de algumas categorias, tentando mostrar como os lugares, profissões e gentílicos mais mencionados podem ser indicadores numa leitura distante.
Concluímos comparando com o trabalho internacional feito na análise de entidades mencionadas de obras literárias, explicando as diferenças e sugerindo trabalho futuro.
Clinical Characterization of Patients With COVID-19 in Primary Care in Catalonia: Retrospective Observational Study
Authors: Mayer Miguel Angel , Vidal-Alaball Josep , Puigdellívol-Sánchez Anna , Marín Gomez Francesc X , Leis Angela , Mendioroz Peña Jacobo 
Published: January 2021

Background
The country of Spain has one of the highest incidences of COVID-19, with more than 1,000,000 cases as of the end of October 2020. Patients with a history of chronic conditions, obesity, and cancer are at greater risk from COVID-19; moreover, concerns surrounding the use of angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin type II receptor blockers (ARBs) and its relationship to COVID-19 susceptibility have increased since the beginning of the pandemic.

Objective
The objectives of this study were to compare the characteristics of patients diagnosed with COVID-19 to those of patients without COVID-19 in primary care; to determine the risk factors associated with the outcome of mortality; and to determine the potential influence of certain medications, such as ACEIs and ARBs, on the mortality of patients with COVID-19.

Methods
An observational retrospective study of patients diagnosed with COVID-19 in the Catalan Central Region of Spain between March 1 and August 17, 2020, was conducted. The data were obtained from the Primary Care Services Information Technologies System of the Catalan Institute of Health in Barcelona, Spain.

Results
The study population included 348,596 patients (aged >15 years) registered in the Primary Care Services Information Technologies System of the Catalan Central Region. The mean age of the patients was 49.53 years (SD 19.42), and 31.17% of the patients were aged ≥60 years. 175,484/348,596 patients (50.34%) were women. A total of 23,844/348,596 patients (6.84%) in the population studied were diagnosed with COVID-19 during the study period, and the most common clinical conditions of these patients were hypertension (5267 patients, 22.1%) and obesity (5181 patients, 21.7%). Overall, 2680/348,596 patients in the study population (0.77%) died during the study period. The number of deaths among patients without COVID-19 was 1825/324,752 (0.56%; mean age 80.6 years, SD 13.3), while among patients diagnosed with COVID-19, the number of deaths was 855/23,844 (3.58%; mean age 83.0 years, SD 10.80) with an OR of 6.58 (95% CI 6.06-7.15).

Conclusions
We observed that women were more likely to contract COVID-19 than men. In addition, our study did not show that hypertension, obesity, or being treated with ACEIs or ARBs was linked to an increase in mortality in patients with COVID-19. Age is the main factor associated with mortality in patients infected with SARS-CoV-2.
CRECIMIENTO ECONÓMICO Y DESIGUALDAD EN ASIA, EUROPA Y AMÉRICA LATINA, 1990-2019
Authors: Cerezo García Verónica , Landa Díaz Heri Oscar 
Published: January 2021

<p>El objetivo de este trabajo es analizar el efecto de la liberalización comercial sobre la productividad, la distribución del ingreso y el crecimiento económico, además de examinar la capacidad de absorción que este proceso ha concedido a los países ante choques externos, como el Covid-19. Empíricamente, tomamos pie en la taxonomía de crecimiento y desigualdad de Fajnzylber (1990) y en un modelo panel para evaluar esta relación en Asia, América Latina y Europa durante el periodo 1990-2019. Los principales resultados muestran: 1) co-movimiento entre crecimiento y equidad en Asia, mientras que en América Latina hay rezagos significativos, y 2) la productividad y la competitividad no precio constituyen el factor dinamizante en Asia y Europa.</p><p align="center"><strong> </strong></p><p align="center">ECONOMIC GROWTH AND INEQUALITY IN ASIA, EUROPE, AND LATIN AMERICA, 1990-2019</p><p align="center"><strong>ABSTRACT</strong></p><p>The paper’s aim is to analyse the effect of trade liberalization on productivity, income distribution and economic growth. The ability of a free-market oriented economy to fence off an exogenous shock such as the Covid-19 pandemic is also dealt with. Following Fajnzylber’s (1990) taxonomy of growth and inequality, we assess the relationship between trade liberalisation, growth and income distribution for a sample set of Asian, Latin American, and European countries over the period 1990-2019. Our main empirical results show that there exist: 1) a co-movement between growth and equality in Asia, but significant lags in both respects prevail in Latin America; 2) productivity and non-price competitiveness are the dynamizing factors in both Asia and Europe</p>
Phenetic Kinship Relationship of Apocynaceae Family Based on Morphological and Anatomical Characters
Authors: Agustiar Ahsanul Buduri , Dewi Masyitoh  , Irda Dwi Fibriana  , Adesilvi Saisatul Khumairoh  , Kurnia Alfi Rianti  , Norma Fitriani  , Muhammad Harissuddin  , Hafidha Asni Akmalia  
Published: January 2021

Background: Biodiversity in Indonesia is so diverse, including in Apocynaceae plants that is why it is important to study the kinship relationship to find out the kinship of Apocynaceae. The purpose of this study was to determine phenetic kinship through morphological and anatomical evidence from four members of the Apocynaceae family. Methods: The method used in this research is descriptive qualitative and quantitative method. The samples in this study were four species of Apocynaceae family members, including Adenium obesum, Plumeria rubra, Catharanthus roseus, and Allamanda cathartica. The indicators used were the morphological traits of stems, leaves, and flowers and the anatomical trait of stomata. Results: The result showed that the phenetic kinship of the four species of the Apocynaceae family member namely Alamanda cathartica had a distant kinship relationship with the other species with a similarity value of 31%. Conclusions: Thus, the familial relationship between species in the Apocynaceae family in terms of morphological and anatomical characters that have a close relationship with Plumeria rubra and Adenium obesum with a similarity value of 44% and the most distant Alamanda cathartica with a similarity value of 31%.
Subchronic Toxicity Test of Indian Almond (Terminalia catappa) Leaves Water Extract on The Liver Histology of Mice (Mus musculus)
Authors: Rudy Nugroho , Ni Cening Sri Puspa  , Retno Aryani  , Widha Prahastika  , Rudianto  , Hetty Manurung  
Published: January 2021

Background: Indian almond (Terminalia catappa) is used as an alternative medicine by the community to treat various diseases. However, limited research has been conducted on the subchronic toxicity of Indian almond water extracts on the liver. Present study aimed to determine the subchronic toxic effects of Indian almond leaf water extract on the liver histology of mice and to evaluate the safe dose of Indian almond leaf water extract with various doses (125, 250, 500, 750 and 1000 mg/kg). Methods: In total of 33 male mice were randomly divided into 11 groups, each group consisting of three mice as replication. For 30 days, mice in five treatment groups were orally treated every day with water extracts of green Indian almond leaf while other five groups were given water extracts of brown Indian almond leaves and compare with control group. At the end of day 30, all mice were sacrificed, and hepar was taken out for histological preparations. The histopathological changes in the liver was observed and assessed based on histological damage in the term of degeneration and necrosis. Results: The administration of green and brown Indian almond water extract at doses of 125, 250, 500, 750 and 1000 mg / kg BB showed a toxic effect on the liver of mice. The toxic effect was due to the high doses, causing a cell swelling damage and increased liver necrosis. Conclusion: Indian almond leaf water extract can cause toxic effects on the liver of mice at the doses implemented.