bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanosensitive Vaginal Epithelial Adenosine Triphosphate Release and Pannexin 1 Channels in Healthy, in Type 1 Diabetic, and in Surgically Castrated Female Mice
Authors: Harroche Jessica , Urban-Maldonado Marcia , Thi Mia M. , Suadicani Sylvia O. 
Published: March 2020

Abstract

Background
Distension of hollow organs is known to release adenosine triphosphate (ATP) from the lining epithelium, which triggers local responses and activates sensory nerves to convey information to the central nervous system. However, little is known regarding participation of ATP and mediators of ATP release, such as Pannexin 1 (Panx1) channels, in mechanisms of vaginal mechanosensory transduction and of changes imposed by diabetes and menopause, conditions associated with vaginal dysfunction and risk for impaired genital arousal.

Aim
To investigate if intravaginal mechanical stimulation triggers vaginal ATP release and if (a) this response involves Panx1 channels and (b) this response is altered in animal models of diabetes and menopause.

Methods
Diabetic Akita female mice were used as a type 1 diabetes (T1D) model and surgical castration (ovariectomy [OVX]) as a menopause model. Panx1-null mice were used to evaluate Panx1 participation in mechanosensitive vaginal ATP release. Vaginal washes were collected from anesthetized mice at baseline (non-stimulated) and at 5 minutes after intravaginal stimulation. For the OVX and Sham groups, samples were collected before surgery and at 4, 12, 22, 24, and 28 weeks after surgery. ATP levels in vaginal washes were measured using the luciferin-luciferase assay. Panx1 mRNA levels in vaginal epithelium were quantified by quantitative polymerase chain reaction.

Outcomes
The main outcome measures are quantification of mechanosensitive vaginal ATP release and evaluation of impact of Panx1 deletion, OVX, and T1D on this response.

Results
Intravaginal mechanical stimulation–induced vaginal ATP release was 84% lower in Panx1-null (P &lt; .001) and 76% lower in diabetic (P &lt; .0001) mice compared with controls and was reduced in a progressive and significant manner in OVX mice when compared with Sham. Panx1 mRNA expression in vaginal epithelium was 44% lower in diabetics than that in controls (P &lt; .05) and 40% lower in OVX than that in the Sham (P &lt; .05) group.

Clinical Translation
Panx1 downregulation and consequent attenuation of mechanosensitive vaginal responses may be implicated in mechanisms of female genital arousal disorder, thereby providing potential targets for novel therapies to manage this condition.

Strengths & Limitations
Using animal models, we demonstrated Panx1 involvement in mechanosensitive vaginal ATP release and effects of T1D and menopause on this response and on Panx1 expression. A limitation is that sex steroid hormone levels were not measured, precluding correlations and insights into mechanisms that may regulate Panx1 expression in the vaginal epithelium.

Conclusions
Panx1 channel is a component of the vaginal epithelial mechanosensory transduction system that is essential for proper vaginal response to mechanical stimulation and is targeted in T1D and menopause.
Responsibility and the Problem of So-Called Marginal Agents
Authors: SVIRSKY LARISA 
Published: March 2020

AbstractPhilosophical views of responsibility often identify responsible agency with capacities such as rationality and self-control. Yet in ordinary life, we frequently hold individuals responsible who are deficient in these capacities, such as children or people with mental illness. The existing literature that addresses these cases has suggested that we merely pretend to hold these agents responsible or that they are responsible to a diminished degree. In this paper, I demonstrate that neither of these approaches is satisfactory, and I offer an alternative focused on the role relationships play in determining whether it is appropriate to hold someone responsible. I argue that relationships are sources of normative expectations about how parties in that relationship ought to behave and that we can be responsible in virtue of being subject to these norms. This is so not only for those who are impaired or immature, but for all of us.
Epidemiological Characteristics on the Clustering Nature of COVID-19 in Qingdao City, 2020: A Descriptive Analysis
Authors: Jia Jing , Hu Xiaowen , Yang Feng , Song Xin , Dong Liyan , Zhang Jingfei , Jiang Fachun , Gao Ruqin 
Published: March 2020

ABSTRACTObjectives:As an emerging infectious disease, COVID-19 has involved many countries and regions. With the further development of the epidemic, the proportion of clusters has increased.Methods:In our study, we collected information on COVID-19 clusters in Qingdao City. The epidemiological characteristics and clinical manifestations were analyzed.Results:Eleven clusters of COVID-19 were reported in Qingdao City between January 29, and February 23, 2020, involving 44 confirmed cases, which accounted for 73.33% of all confirmed cases. From January 19 to February 2, 2020, the cases mainly concentrated in the district that had many designated hospitals. Patients aged 20-59 y old accounted for the largest proportion (68.18%) of cases; the male-to-female sex ratio was 0.52:1. Three cases were infected from exposure to confirmed cases. The average incubation period was 6.28 d. The median number of cases per cluster was 4, and the median duration time was 6 d. The median cumulative number of exposed persons was 53.Conclusion:More attention should be paid to the epidemic of clusters in prevention and control of COVID-19. In addition to isolating patients, it is essential to track, screen, and isolate those who have come in close contact with patients. Self-isolation is the key especially for healthy people in the epidemic area.
Probing the relationship between BTBD9 and MEIS1 in C. elegans and mouse
Authors: Lyu Shangru , Doroodchi Atbin , Sheng Yi , DeAndrade Mark P. , Yang Youfeng , Liu Yuning , Miller Michael A. , Xiao Rui , Li Yuqing 
Published: March 2020

AbstractRestless legs syndrome (RLS) is a neurological disorder characterized by an urge to move and uncomfortable sensations. Genetic studies have identified polymorphisms in up to 19 risk loci, including MEIS1 and BTBD9. Rodents deficient in either homolog show RLS-like phenotypes. However, whether MEIS1 and BTBD9 interact in vivo is unclear. Here, with C. elegans, we observed that the hyperactive egg-laying behavior caused by loss of BTBD9 homolog was counteracted by knockdown of MEIS1 homolog. This was further investigated in mutant mice with Btbd9, Meis1, or both knocked out. The double knockout mice showed an earlier onset of the motor deficit in a wheel running test but did not have increased sensitivity to heat stimuli as observed in single knock outs. Meis1 protein level was not influenced by Btbd9 deficiency, and Btbd9 transcription was not affected by Meis1 haploinsufficiency. Our results demonstrate that MEIS1 and BTBD9 do not regulate each other.
SANOM-HOBBIT: simulated annealing-based ontology matching on HOBBIT platform
Authors: Mohammadi Majid , Hofman Wout , Tan Yao-Hua 
Published: March 2020

Abstract
Ontology alignment is an important and inescapable problem for the interconnections of two ontologies stating the same concepts. Ontology alignment evaluation initiative (OAEI) has been taken place for more than a decade to monitor and help the progress of the field and to compare systematically existing alignment systems. As of 2018, the evaluation of systems is partly transitioned to the HOBBIT platform. This paper contains the description of our alignment system, simulated annealing-based ontology matching (SANOM), and its adaption into the HOBBIT platform. The outcomes of SANOM on the HOBBIT for several OAEI tracks are reported, and the results are compared with other competing systems in the corresponding tracks.
Meningococcal carriage in Norwegian teenagers: strain characterisation and assessment of risk factors
Authors: Watle S. V. , Caugant D. A. , Tunheim G. , Bekkevold T. , Laake I. , Brynildsrud O. B. , Næss L. M. 
Published: March 2020

Abstract
Teenagers have a higher risk of invasive meningococcal disease (IMD) than the general population. This cross-sectional study aimed to characterise strains of Neisseria meningitidis circulating among Norwegian teenagers and to assess risk factors for meningococcal carriage. Oropharyngeal swabs were collected from secondary-school students in southeastern Norway in 2018–2019. Meningococcal isolates were characterised using whole genome sequencing. Risk factors for meningococcal carriage were assessed from questionnaire data. Samples were obtained from 2296 12–24-year-olds (majority 13–19-year-olds). N. meningitidis was identified in 167 (7.3%) individuals. The highest carriage rate was found among 18-year-olds (16.4%). Most carriage isolates were capsule null (40.1%) or genogroup Y (33.5%). Clonal complexes cc23 (35.9%) and cc198 (32.3%) dominated and 38.9% of carriage strains were similar to invasive strains currently causing IMD in Norway. Use of Swedish snus (smokeless tobacco) (OR 1.56, 95% CI 1.07–2.27), kissing >two persons/month (OR 2.76, 95% CI 1.49–5.10) and partying >10 times/3months (OR 3.50, 95% CI 1.45–8.48) were associated with carriage, while age, cigarette smoking, sharing of drinking bottles and meningococcal vaccination were not. The high meningococcal carriage rate among 18-year-olds is probably due to risk-related behaviour. Use of Swedish snus is possibly a new risk factor for meningococcal carriage. Almost 40% of circulating carriage strains have invasive potential.
Risk of bacteremia in patients presenting with shaking chills and vomiting - a prospective cohort study
Authors: Holmqvist M. , Inghammar M. , Påhlman L. I. , Boyd J. , Åkesson P. , Linder A. , Kahn F. 
Published: March 2020

Abstract
Chills and vomiting have traditionally been associated with severe bacterial infections and bacteremia. However, few modern studies have in a prospective way evaluated the association of these signs with bacteremia, which is the aim of this prospective, multicenter study. Patients presenting to the emergency department with at least one affected vital sign (increased respiratory rate, increased heart rate, altered mental status, decreased blood pressure or decreased oxygen saturation) were included. A total of 479 patients were prospectively enrolled. Blood cultures were obtained from 197 patients. Of the 32 patients with a positive blood culture 11 patients (34%) had experienced shaking chills compared with 23 (14%) of the 165 patients with a negative blood culture, P = 0.009. A logistic regression was fitted to show the estimated odds ratio (OR) for a positive blood culture according to shaking chills. In a univariate model shaking chills had an OR of 3.23 (95% CI 1.35–7.52) and in a multivariate model the OR was 5.9 (95% CI 2.05–17.17) for those without prior antibiotics adjusted for age, sex, and prior antibiotics. The presence of vomiting was also addressed, but neither a univariate nor a multivariate logistic regression showed any association between vomiting and bacteremia. In conclusion, among patients at the emergency department with at least one affected vital sign, shaking chills but not vomiting were associated with bacteremia.
Clinical characteristics and factors influencing the prognosis of Behçet’s disease complicated with vascular involvement
Authors: Lin Chen-Hong , Luo Dan , Ma Hai-Fen , Shen Yan , Zou Jun , Guan Jian-Long 
Published: March 2020

Summary: Background: Vascular Behçet's disease (VBD) might involve all sizes of arterial and venous vessels. Major vascular involvement caused the primary death in Behçet's syndrome (BS). We aimed to investigate the clinical characteristics and factors influencing the prognosis of VBD. Patients and methods: A retrospective analysis of the prospectively collected data of the Shanghai BS database from October 2012 to October 2018 was conducted. Patients who were diagnosed with BS and merged with venous thrombosis, arterial aneurysms, and arterial stenosis/occlusions were enrolled. Results: There were 47 patients with vascular involvement among 836 BS patients, 38 males and 9 females. The numbers of patients with venous thrombosis, arterial aneurysm, and arterial stenosis/occlusion were 25 (53.2 %), 21 (44.7 %), and 12 (25.5 %), respectively. Nearly half of the venous thromboses were located in limbs (n = 22, 46.8 %). Arterial aneurysm was the main form of arterial lesion. Most of the patients (93.6 %) were treated with corticosteroids and immunosuppressants. Late onset of BS or with arterial involvement had lower treatment response. Therapy with biological agents had significantly better results than that in the group without biological treatment (94.1 % vs. 80 %, P = 0.005). Conclusions: VBD showed a male preponderance and more than half of the patients presented with venous thrombosis. Late onset and arterial involvement were associated with poor prognosis. Therapy with biological agents is a viable alternative treatment to improve the prognosis.
miR-4651 inhibits cell proliferation of gingival mesenchymal stem cells by inhibiting HMGA2 under nifedipine treatment
Authors: Han Xiao , Yang Ruzhuang , Yang Haoqing , Cao Yangyang , Han Nannan , Zhang Chen , Shi Ruitang , Zhang Zhengting , Fan Zhipeng 
Published: March 2020

AbstractDrug-induced gingival overgrowth (DIGO) is recognized as a side effect of nifedipine (NIF); however, the underlying molecular mechanisms remain unknown. In this study, we found that overexpressed miR-4651 inhibits cell proliferation and induces G0/G1-phase arrest in gingival mesenchymal stem cells (GMSCs) with or without NIF treatment. Furthermore, sequential window acquisition of all theoretical mass spectra (SWATH-MS) analysis, bioinformatics analysis, and dual-luciferase report assay results confirmed that high-mobility group AT-hook 2 (HMGA2) is the downstream target gene of miR-4651. Overexpression of HMGA2 enhanced GMSC proliferation and accelerated the cell cycle with or without NIF treatment. The present study demonstrates that miR-4651 inhibits the proliferation of GMSCs and arrests the cell cycle at the G0/G1 phase by upregulating cyclin D and CDK2 while downregulating cyclin E through inhibition of HMGA2 under NIF stimulation. These findings reveal a novel mechanism regulating DIGO progression and suggest the potential of miR-4651 and HMGA2 as therapeutic targets.
Coriander Genomics Database: a genomic, transcriptomic, and metabolic database for coriander
Authors: Song Xiaoming , Nie Fulei , Chen Wei , Ma Xiao , Gong Ke , Yang Qihang , Wang Jinpeng , Li Nan , Sun Pengchuan , Pei Qiaoying , Yu Tong , Hu Jingjing , Li Xinyu , Wu Tong , Feng Shuyan , Li Xiu-Qing , Wang Xiyin 
Published: March 2020

AbstractCoriander (Coriandrum sativum L.), also known as cilantro, is a globally important vegetable and spice crop. Its genome and that of carrot are models for studying the evolution of the Apiaceae family. Here, we developed the Coriander Genomics Database (CGDB, http://cgdb.bio2db.com/) to collect, store, and integrate the genomic, transcriptomic, metabolic, functional annotation, and repeat sequence data of coriander and carrot to serve as a central online platform for Apiaceae and other related plants. Using these data sets in the CGDB, we intriguingly found that seven transcription factor (TF) families showed significantly greater numbers of members in the coriander genome than in the carrot genome. The highest ratio of the numbers of MADS TFs between coriander and carrot reached 3.15, followed by those for tubby protein (TUB) and heat shock factors. As a demonstration of CGDB applications, we identified 17 TUB family genes and conducted systematic comparative and evolutionary analyses. RNA-seq data deposited in the CGDB also suggest dose compensation effects of gene expression in coriander. CGDB allows bulk downloading, significance searches, genome browser analyses, and BLAST searches for comparisons between coriander and other plants regarding genomics, gene families, gene collinearity, gene expression, and the metabolome. A detailed user manual and contact information are also available to provide support to the scientific research community and address scientific questions. CGDB will be continuously updated, and new data will be integrated for comparative and functional genomic analysis in Apiaceae and other related plants.
The basic helix-loop-helix transcription factor MdbHLH3 modulates leaf senescence in apple via the regulation of dehydratase-enolase-phosphatase complex 1
Authors: Hu Da-Gang , Sun Cui-Hui , Zhang Quan-Yan , Gu Kai-Di , Hao Yu-Jin 
Published: March 2020

AbstractBasic helix−loop−helix (bHLH) domain-containing transcription factors are known for their roles in regulating various plant growth and developmental processes. Previously, we showed that MdbHLH3 from apple (Malus domestica) has multiple functions, modulating both anthocyanin biosynthesis and cell acidification. Here, we show that MdbHLH3 also regulates ethylene biosynthesis and leaf senescence by promoting the expression of dehydratase-enolase-phosphatase complex 1 (MdDEP1). Therefore, we propose a model whereby MdbHLH3 acts as a crucial factor that modulates anthocyanin biosynthesis and cell acidification in addition to fruit ripening and leaf senescence by regulating distinct target genes.
Novel ARX mutation identified in infantile spasm syndrome patient
Authors: Takeshita Yohei , Ohto Tatsuyuki , Enokizono Takashi , Tanaka Mai , Suzuki Hisato , Fukushima Hiroko , Uehara Tomoko , Takenouchi Toshiki , Kosaki Kenjiro , Takada Hidetoshi 
Published: March 2020

AbstractWe report a 7-year-old boy with infantile spasms caused by a novel mutation in theAristaless-related homeobox(ARX) gene. He showed infantile spasms and hypsarrhythmia on electroencephalogram from early infancy. Brain MRI did not reveal severe malformation of the brain except mild hypoplasia of the corpus callosum. Two-fold adrenocorticotropic hormone (ACTH) therapy failed to control the seizures, and ketogenic diet therapy and multi-antiepileptic drug therapy were required as he showed intractable daily tonic-clonic seizures. Exome sequencing identified a hemizygous mutation in theARXgene, NG_008281.1(ARX_v001):c.1448 + 1 G > A, chrX: 25025227 C > T (GRCh37). To our knowledge, this mutation has not been reported previously.
Modification of Adenovirus vaccine vector-induced immune responses by expression of a signalling molecule
Authors: Rollier Christine S. , Spencer Alexandra J. , Sogaard Karen Colbjorn , Honeycutt Jared , Furze Julie , Bregu Migena , Gilbert Sarah C. , Wyllie David , Hill Adrian V. S. 
Published: March 2020

AbstractAdenoviral vectors are being developed as vaccines against infectious agents and tumour-associated antigens, because of their ability to induce cellular immunity. However, the protection afforded in animal models has not easily translated into primates and clinical trials, underlying the need for improving adenoviral vaccines-induced immunogenicity. A Toll-like receptor signalling molecule, TRAM, was assessed for its ability to modify the immune responses induced by an adenovirus-based vaccine. Different adenovirus vectors either expressing TRAM alone or co-expressing TRAM along with a model antigen were constructed. The modification of T-cell and antibody responses induced by TRAM was assessed in vivo in mice and in primates. Co-expression of TRAM and an antigen from adenoviruses increased the transgene-specific CD8+ T cell responses in mice. Similar effects were seen when a TRAM expressing virus was co-administered with the antigen-expressing adenovirus. However, in primate studies, co-administration of a TRAM expressing adenovirus with a vaccine expressing the ME-TRAP malaria antigen had no significant effect on the immune responses. While these results support the idea that modification of innate immune signalling by genetic vectors modifies immunogenicity, they also emphasise the difficulty in generalising results from rodents into primates, where the regulatory pathway may be different to that in mice.
Scalable diagnostic screening of mild cognitive impairment using AI dialogue agent
Authors: Tang Fengyi , Uchendu Ikechukwu , Wang Fei , Dodge Hiroko H. , Zhou Jiayu 
Published: March 2020

AbstractThe search for early biomarkers of mild cognitive impairment (MCI) has been central to the Alzheimer’s Disease (AD) and dementia research community in recent years. To identify MCI status at the earliest possible point, recent studies have shown that linguistic markers such as word choice, utterance and sentence structures can potentially serve as preclinical behavioral markers. Here we present an adaptive dialogue algorithm (an AI-enabled dialogue agent) to identify sequences of questions (a dialogue policy) that distinguish MCI from normal (NL) cognitive status. Our AI agent adapts its questioning strategy based on the user’s previous responses to reach an individualized conversational strategy per user. Because the AI agent is adaptive and scales favorably with additional data, our method provides a potential avenue for large-scale preclinical screening of neurocognitive decline as a new digital biomarker, as well as longitudinal tracking of aging patterns in the outpatient setting.
Enhancing photoluminescence of carbon quantum dots doped PVA films with randomly dispersed silica microspheres
Authors: Zhao Xun , Wang Ailin , Gao Sili , Yan Duanting , Guo Wanying , Xu Yingyue , Meng Yanli , Wang Chunliang , Shan Guiye 
Published: March 2020

AbstractAs a kind of excellent photoluminescent material, carbon quantum dots have been extensively studied in many fields, including biomedical applications and optoelectronic devices. They have been dispersed in polymer matrices to form luminescent films which can be used in LEDs, displays, sensors, etc. Owing to the total internal reflection at the flat polymer/air interfaces, a significant portion of the emitted light are trapped and dissipated. In this paper, we fabricate free standing flexible PVA films with photoluminescent carbon quantum dots embedded in them. We disperse silica microspheres at the film surfaces to couple out the total internal reflection. The effects of sphere densities and diameters on the enhancement of photoluminescence are experimentally investigated with a homemade microscope. The enhancement of fluorescence intensity is as high as 1.83 when the film is fully covered by spheres of 0.86 $${\boldsymbol{\mu }}$$
µ
m diameter. It is worth noting that the light extraction originates from rather the scattering of individual spheres than the diffraction of ordered arrays. The mechanism of scattering is confirmed by numerical simulations. The simulated results show that the evanescent wave at the flat PVA/air interface can be effectively scattered out of the film.