bluuebunny/crossref_metadata_2025_split huggingface dataset:
Requirement of Mucosa‐Associated Lymphoid Tissue Lymphoma Translocation Protein 1 Protease Activity for Fcγ Receptor–Induced Arthritis, but Not Fcγ Receptor–Mediated Platelet Elimination, in Mice
Authors: Martin Kea , Touil Ratiba , Cvijetic Grozdan , Israel Laura , Kolb Yeter , Sarret Sophie , Valeaux Stéphanie , Degl'Innocenti Elena , Le Meur Thomas , Caesar Nadja , Bardet Maureen , Beerli Christian , Zerwes Hans‐Guenter , Kovarik Jiri , Beltz Karen , Schlapbach Achim , Quancard Jean , Régnier Catherine H. , Bigaud Marc , Junt Tobias , Wieczorek Grazyna , Isnardi Isabelle , Littlewood‐Evans Amanda , Bornancin Frédéric , Calzascia Thomas 
Published: January 2020

ObjectiveFcγ receptors (FcγR) play important roles in both protective and pathogenic immune responses. The assembly of the CBM signalosome encompassing caspase recruitment domain–containing protein 9, B cell CLL/lymphoma 10, and mucosa‐associated lymphoid tissue lymphoma translocation protein 1 (MALT‐1) is required for optimal FcγR‐induced canonical NF‐κB activation and proinflammatory cytokine release. This study was undertaken to clarify the relevance of MALT‐1 protease activity in FcγR‐driven events and evaluate the therapeutic potential of selective MALT‐1 protease inhibitors in FcγR‐mediated diseases.MethodsUsing genetic and pharmacologic disruption of MALT‐1 scaffolding and enzymatic activity, we assessed the relevance of MALT‐1 function in murine and human primary myeloid cells upon stimulation with immune complexes (ICs) and in murine models of autoantibody‐driven arthritis and immune thrombocytopenic purpura (ITP).ResultsMALT‐1 protease function is essential for optimal FcγR‐induced production of proinflammatory cytokines by various murine and human myeloid cells stimulated with ICs. In contrast, MALT‐1 protease inhibition did not affect the Syk‐dependent, FcγR‐mediated production of reactive oxygen species or leukotriene B4. Notably, pharmacologic MALT‐1 protease inhibition in vivo reduced joint inflammation in the murine K/BxN serum–induced arthritis model (mean area under the curve for paw swelling of 45.42% versus 100% in control mice; P = 0.0007) but did not affect platelet depletion in a passive model of ITP.ConclusionOur findings indicate a specific contribution of MALT‐1 protease activity to FcγR‐mediated events and suggest that MALT‐1 protease inhibitors have therapeutic potential in a subset of FcγR‐driven inflammatory disorders.
Accretion‐induced collapse to third family compact stars as trigger for eccentric orbits of millisecond pulsars in binaries
Authors: Alvarez‐Castillo David Edwin , Antoniadis John , Ayriyan Alexander , Blaschke David , Danchev Victor , Grigorian Hovik , Largani Noshad Khosravi , Weber Fridolin 
Published: January 2020

AbstractA numerical rotating neutron star solver is used to study the temporal evolution of accreting neutron stars using a multi‐polytrope model for the nuclear equation of state named ACB5. The solver is based on a quadrupole expansion of the metric, but confirms the results of previous works, revealing the possibility of an abrupt transition of a neutron star from a purely hadronic branch to a third‐family branch of stable hybrid stars, passing through an unstable intermediate branch. The accretion is described through a sequence of stationary rotating stellar configurations which lose angular momentum through magnetic dipole emission, while, at the same time, gaining angular momentum through mass accretion. The model has several free parameters which are inferred from observations. The mass accretion scenario is studied in dependence on the effectiveness of angular momentum transfer which determines at which spin frequency the neutron star will become unstable against gravitational collapse to the corresponding hybrid star on the stable third‐family branch. It is conceivable that the neutrino burst which accompanies the deconfinement transition may trigger a pulsar kick which results in the eccentric orbit. A consequence of the present model is the prediction of a correlation between the spin frequency of the millisecond pulsar in the eccentric orbit and its mass at birth.
Approaching Reactive KFePO4 Phase for Potassium Storage by Adopting an Advanced Design Strategy
Authors: Sultana Irin , Rahman M. Mokhlesur , Mateti Srikanth , Sharma Neeraj , Huang Shaoming , Chen Ying 
Published: January 2020

AbstractPristine KFePO4 is electrochemically inactive towards potassium, which suggests that K+ ion migration is unfavorable in the KFePO4 structure. Therefore, it is a challenge to create a reactive structure of KFePO4 towards reversible potassium insertion/extraction. In this study, we have fabricated reactive KFePO4 phase by adopting high‐energy ball milling technique where tiny nanoparticles (∼1.0‐1.5 nm) of KFePO4 are created and embedded in the Super P carbon black matrix, which provides conductivity and helps to stabilize the electrode structure. The combination of nano‐sizing and the conductive matrix appears to activate electrochemical reactivity. The obtained carbon incorporated KFePO4 material appears to be amorphous. The electrode produces an average operational potential of ∼2.5 V (vs K+/K) with high capacity retention of 90 mAh g−1. It is anticipated that this work could pave the way for further studies on cathodes and generate huge attention to the role of ball milling and particle size control on electrochemical activity of KFePO4 materials for the development of PIBs.
Production and purification of xylitol by Scheffersomyces amazonenses via sugarcane hemicellulosic hydrolysate
Authors: Silva Débora D.V. , Dussán Kelly J. , Idarraga Angela , Grangeiro Luana , Silva Silvio S. , Cardona Carlos A. , Quintero Julián , Felipe Maria G.A. 
Published: January 2020

AbstractXylitol was produced by Scheffersomyces amazonensis UFMG‐HMD‐26.3 in hemicellulosic hydrolysate obtained from a mixture of sugarcane bagasse and straw (1:1) in batch fermentation, and was recovered using supercritical fluid. The xylitol produced was 20.11 g L−1 and the extraction approach reached a xylitol recovery of 40.51% at a high purity level of 99.59%. An analysis of energy consumption for the xylitol production process showed that xylitol production from sugarcane biomass hemicellulosic hydrolysate is feasible, highlighting the potential use of this biomass in sustainable xylitol production and supercritical fluids as a robust proposal for xylitol separation. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd
“To hope was to expect”: The impact of perspective taking and forecast type on wishful thinking
Authors: Rose Jason P. , Aspiras Olivia 
Published: January 2020

AbstractWhen forecasting future outcomes, people tend to believe that the outcomes they want to happen are also likely to happen. Despite numerous attempts, few systematic factors have been identified that consistently and robustly reduce wishful thinking (WT) effects. Using elections and sporting event outcomes as contexts, three experiments examined whether taking the perspective of a political rival or opposing fan reduced WT effects. We also examined whether making deliberative (vs. intuitive‐based) forecasts was associated with lower WT effects. Online adult samples of U.S. citizens from Mechanical Turk and U.S. college students provided their preferences and forecasts for the U.S. presidential election (Experiments 1 and 2) and a sports competition outcome (Experiment 3). Critically, some participants received perspective taking prompts immediately before providing forecasts. First, results revealed reductions in WT effects when participants engaged in perspective taking. Interestingly, this effect only emerged when intuitive‐based forecasts were made first (Experiment 3). Second, intuitive‐based forecasts revealed stronger evidence of WT effects. Finally, we found that perspective taking and forming forecasts deliberately promoted a shift in focus away from preferences and toward a consideration of the relative strengths and weaknesses of the entities (i.e., candidates and teams). Theoretical implications for understanding WT effects and applied implications for developing interventions are discussed.
Novel spectrofluorimetric quantification of linagliptin in biological fluids exploiting its interaction with 4‐chloro‐7‐nitrobenzofurazan
Authors: Aref Heba A. , Hammad Sherin F. , Elgawish Mohamed Saleh , Darwish Khaled M. 
Published: January 2020

AbstractDirect determination of linagliptin (LIN) using fluorimetry has been limited because LIN releases a very weak fluorescence signal. Accordingly, it should be derivatized first with a fluorogenic reagent to enhance its fluorescence and consequentially the sensitivity required for its bioanalysis. This is the first description of a spectrofluorimetric method for LIN quantification in human plasma. The suggested method exploits the nucleophilic nature of its amino group to react with 4‐chloro‐7‐nitrobenzofurazan (NBD‐Cl) in borate buffer at pH 8.5 to yield a strong fluorescent product with excitation and emission wavelengths of 459 and 529 nm, respectively. Experimental variables concerning the conditions of reaction and fluorescence intensity were carefully investigated and optimized. The linearity range was from 1.0–100 ng ml−1 with a lower detection limit and a lower quantification limit of 0.60 ng ml−1 and 1.82 ng ml−1, respectively. Validation of the suggested method has been accomplished and the application to LIN analysis in commercial tablets as well as in human plasma resulted in a mean per cent recovery of 100.12 ± 1.57 and 99.65 ± 1.22, respectively. The developed method was proven to be a promising, simple and fast alternative bioanalytical method for LIN quantification in clinical and bioequivalence research.
Integrated analysis of a ceRNA network reveals potential prognostic lncRNAs in gastric cancer
Authors: Qi Mingran , Yu Bingxin , Yu Huiyuan , Li Fan 
Published: January 2020

AbstractLong noncoding RNAs (lncRNAs) have important biological functions as competing endogenous RNAs (ceRNAs) in tumors, yet the functions and regulatory mechanisms of lncRNA‐related ceRNAs in gastric cancer have not been fully elucidated. In this study, we constructed a lncRNA‐miRNA‐mRNA ceRNA network and identified potential lncRNA biomarkers in gastric cancer. Basing on the RNA profiles downloaded from The Cancer Genome Atlas (TCGA) platform, the gastric cancer‐specific differentially expressed lncRNAs, miRNAs, and mRNAs were screened for constructing a ceRNA network using bioinformatic tools. The enrichment analysis of the biological processes in Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathways was performed on the ceRNA‐related DEmRNAs. According to the modularization of protein‐protein interaction (PPI) network, we extracted a ceRNA subnetwork and analyzed the correlation between the expression of the lncRNAs involved and specific clinical features of patients. Next, the expression of highly up‐regulated in liver cancer (HULC) and RP11‐314B1.2 showed significant changes in several pathological processes involved in gastric cancer, and nine lncRNAs were found to be correlated with the overall survival of patients with gastric cancer. Through the univariate and multivariate Cox regression analyses, two lncRNAs (LINC00106 and RP11‐999E24.3) were identified and utilized to establish a risk score model for assessing the prognosis of patients. The analysis results were also partially verified using quantitative real‐time PCR. The findings from this study indicate that HULC, RP11‐314B1.2, LINC00106, and RP11‐999E24.3 could be considered as potential therapeutic targets or prognostic biomarkers in gastric cancer, and provide a new perspective for cancer pathogenesis research.
The microRNA‐155 mediates hepatitis B virus replication by reinforcing SOCS1 signalling–induced autophagy
Authors: Chen Liyan , Ming Xiaoyu , Li Wensong , Bi Manru , Yan Bingzhu , Wang Xiaoren , Yang Pengfei , Yang Baoshan 
Published: January 2020

As small conserved RNAs without a coding function, microRNAs are expressed in multicellular organisms and contribute to the modulation of multiple cellular reactions, such as viral replication, as well as autophagy. microRNAs can regulate host gene expression and inhibit or reinforce hepatitis B virus (HBV) replication. Hepatic cells express miR‐155 noticeably. Consequently, our study explored miR‐155 modulation of HBV replication and investigated the potential mechanism involved. miR‐155 was inhibited on HBV infection. miR‐155 transfection remarkably reinforced HBV replication, antigen expression, and progeny secretion in HepG2215 cells. Moreover, miR‐155 impaired the inhibition of the cytokine signalling 1 (SOCS1)/Akt/mTOR axis and reinforced HepG2215 autophagy. Additionally, the autophagy inhibitor (3‐MA) eliminated HBsAg secretion triggered by miR‐155. Taken together, miR‐155 reinforced HBV replication by reinforcing SOCS1‐triggered autophagy.Significance of the studyThe research studied the potential mechanism involved in HBV replication and miR‐155 that miR‐155 reinforces HBV replication by reinforcing the SOCS1/Akt/mTOR axis–stimulated autophagy, and therefore, it can provide medical practitioners with the inspiration that chronic HBV might be cured or improved by regulating the activation of miR‐155 in cells. In the study, the experiments show that autophagy inhibitors (3‐MA) counteracted miR‐155 contribution to HBV replication, and it might be a practicable way to improve HBV through some therapies that can repress the autophagy in related cells.
Quantitative MS‐Based Proteomics: Comparing the MCF‐7 Cellular Response to Hypoxia and a 2‐Oxoglutarate Analogue
Authors: Bush Jacob T. , Chan Mun Chiang , Mohammed Shabaz , Schofield Christopher J. 
Published: January 2020

AbstractThe hypoxia‐inducible factors (HIFs) are key transcription factors in determining cellular responses involving alterations in protein levels in response to limited oxygen availability in animal cells. 2‐Oxoglutarate‐dependent oxygenases play key roles in regulating levels of HIF and its transcriptional activity. We describe MS‐based proteomics studies in which we compared the results of subjecting human breast cancer MCF‐7 cells to hypoxia or treating them with a cell‐penetrating derivative (dimethyl N‐oxalylglycine; DMOG) of the stable 2OG analogue N‐oxalylglycine. The proteomic results are consistent with reported transcriptomic analyses and support the proposed key roles of 2OG‐dependent HIF prolyl‐ and asparaginyl‐hydroxylases in the hypoxic response. Differences between the data sets for hypoxia and DMOG might reflect context‐dependent effects or HIF‐independent effects of DMOG.
Percutaneous transluminal angioplasty with selective stenting for the treatment of renal artery stenosis caused by fibromuscular dysplasia: 18 years' experience from the China Center for Cardiovascular Disease
Authors: Chen Yang , Dong Hui , Jiang Xiongjing , Deng Yu , Zou Yubao , Che Wuqiang , Xiong Hongliang , Xu Bo , Yang Yuejin , Gao Runlin 
Published: January 2020

AbstractObjectivesThis study aimed to evaluate the safety and efficiency of percutaneous transluminal angioplasty (PTA) with selective stenting treating Chinese patients with renal artery stenosis (RAS) due to fibromuscular dysplasia (FMD).BackgroundEndovascular data of patients with RAS caused by FMD are scarce in China.MethodsClinical data of 105 hypertensive patients with RAS caused by FMD underwent endovascular therapy at a single‐site between June 2001 and October 2018 were analyzed retrospectively. Baseline blood pressure, use of antihypertensive medications, renal function, and adverse events at a 1‐year follow‐up were evaluated.ResultsThe patients were aged between 10 and 64 years (mean age 26.7 ± 8.2 years) and 52 (49.5%) were female. In total, 105 patients undergoing endovascular therapy involving 124 RASs. Thirty (24.2%), sixty‐four (51.6%), and thirty (24.2%) RASs were multifocal, unifocal, and tubular types, respectively. Ninety‐four (89.5%) and eleven (10.5%) patients underwent PTA alone and PTA plus stenting, respectively. The technical success rate for endovascular therapy was 95.2% (100/105) without severe complications. During 1‐year follow‐up (n = 100), the mean systolic and diastolic blood pressure decreased from 157.6 ± 17.5 and 102.3 ± 14.2 to 129.6 ± 12.3 and 81.3 ± 11.1 mmHg, respectively, and the number of antihypertensive medications reduced from 2.2 ± 1.2 to 0.8 ± 1.0 (all p < .001). The cure rate and improved rate of blood pressure was 49.0 and 40.0%, respectively. The serum creatinine levels remained stable. The primary and secondary restenosis rate was 13.4 and 5.8%, respectively.ConclusionEndovascular therapy for treating RAS caused by FMD was technically safe and effective for reducing blood pressure in Chinese patients.
Evaluation of a risk index for predicting short‐term and long‐term outcomes in patients with ST‐elevation myocardial infarction
Authors: Shao Chunli , Wang Jingjia , Li Ping , Yang Jingang , Wang Wenyao , Wang Yang , Zhao Yanyan , Ni Lin , Tian Jian , Zhang Kuo , Gao Jun , Tang Yi‐Da , Yang Yuejin 
Published: January 2020

AbstractObjectiveThis study aimed to evaluate the usefulness of the admission risk index (RI) to predict short‐term and long‐term outcomes in a broad population with ST‐elevation myocardial infarction (STEMI) using data from the Chinese Acute Myocardial Infarction Registry.BackgroundThe RI was developed as a simple tool to predict risk of death in STEMI patients. The performance in predicting short‐term and long‐term risk of death in Chinese patients receiving percutaneous coronary intervention and conservative treatment for STEMI remains unclear.MethodsAge, heart rate (HR), and systolic blood pressure (SBP) were used to calculate RI using (HR×[age/10]2)/SBP. We used the prediction tool to predict mortality over 12 months.ResultsTheC‐index of the admission RI for predicting in‐hospital, 1‐, 6‐, and 12‐months mortality were 0.78, 0.78, 0.78, and 0.77, respectively, compared with 0.75 of the Global Registry in Acute Coronary Events score. Based on the receiver operating characteristic curve analysis, the RI was categorized into quintiles for convenient clinical use, and it revealed a nearly 15‐fold gradient of increasing mortality from 2.29 to 32.5% (p < .0001) while RI >34 had the highest mortality. By categorizing into five different risk groups, the short‐term and long‐term mortality of patients receiving different treatments could be distinguished.ConclusionsRI based on three routine variables and easily calculated by any medical practitioner is useful for predicting in‐hospital and long‐term mortality in patients with STEMI at the initial consultation with clinicians.
Northwell intracoronary brachytherapy for the treatment of recurrent drug eluting stent in‐stent restenosis (NITDI study group)
Authors: Meraj Perwaiz M. , Patel Krunalkumar , Patel Amitkumar , Doshi Rajkumar , Srinivas Guruprasad , Jauhar Rajiv , Kaplan Barry , Garzon Ruby , Sharma Anurag , Cao Yijian , Diaz Molina Ferney , Sharma Rajiv 
Published: January 2020

AbstractObjectiveThe purpose of this study is to report on safety, short‐term and long‐term clinical efficacy following intracoronary brachytherapy (ICBT) for restenosis (ISR) in patients with drug eluting stents (DES).BackgroundICBT is an effective treatment for ISR of bare metal stents (BMS) but its utilization has waned due to the advent of DES. ISR following DES occurs at a frequency of 8% or greater.MethodA retrospective analysis was performed on patients treated on an institutional review board (IRB) approved protocol using ICBT for DES ISR between January 2011 and October 2016. All patients were followed for 24 months for procedural complications, mortality, clinical ISR/target lesion revascularization (TLR) and stroke.ResultsA total of 290 patients were identified with a mean age of 66.6 years. All of them had high rates of typical coronary artery disease risk factors. Our primary outcome, composite of in‐hospital mortality, myocardial infarction (MI), safety outcomes and procedural failure was noted in 1(0.3%) patient who had a MI. No other secondary outcome was noted in‐hospital. At 1‐year follow up, 12.4% patients had ISR, 1.7% patients died, and 1 (0.3%) had ischemic stroke. At 2‐year, 14.7% had ISR, and total 6 (2.1%) patients had MI.ConclusionICBT demonstrates excellent technical success rates for treatment, safety, and reasonable efficacy over 2‐years to be free from recurrent clinical ISR. This study represents the largest ICBT data for DES ISR to date among very complex lesion subsets, however, more prospective data will be needed to determine the optimal patient for treatment.
Organophotoredox‐Mediated Amide Synthesis by Coupling Alcohol and Amine through Aerobic Oxidation of Alcohol
Authors: Shah Sk. Sheriff , Shee Maniklal , Venkatesh Yarra , Singh Amit Kumar , Samanta Samya , Singh N. D. Pradeep 
Published: January 2020

AbstractThe combination of an organic photocatalyst [4CzIPN (1,2,3,5‐tetrakis(carbazol‐9‐yl)‐4,6 dicyanobenzene) or 5MeOCzBN (2,3,4,5,6‐pentakis(3,6‐dimethoxy‐9 H‐carbazol‐9‐yl)benzonitrile)], quinuclidine, and tetra‐n‐butylammonium phosphate (hydrogen‐bonding catalyst) was employed for amide bond formations. The hydrogen‐bonded OH group activated the adjacent C−H bond of alcohols towards hydrogen atom transfer (HAT) by a radical species. The quinuclidinium radical cation, generated through single‐electron oxidation of quinuclidine by the photocatalyst, employed to abstract a hydrogen atom from the α‐C−H bond of alcohols selectively due to a polarity effect‐produced α‐hydroxyalkyl radical, which subsequently converted to the corresponding aldehyde under aerobic conditions. Then the coupling of the aldehyde and an amine formed a hemiaminal intermediate that upon photocatalytic oxidation produced the amide.
Photo Cross‐Linking Probes Containing ϵ‐N‐Thioacyllysine and ϵ‐N‐Acyl‐(δ‐aza)lysine Residues
Authors: Bæk Michael , Martín‐Gago Pablo , Laursen Jonas S. , Madsen Julie L. H. , Chakladar Saswati , Olsen Christian A. 
Published: January 2020

AbstractPosttranslational modifications (PTMs) are important in the regulation of protein function, trafficking, localization, and marking for degradation. This work describes the development of peptide activity/affinity‐based probes for the discovery of proteins that recognize novel acyl‐based PTMs on lysine residues in the proteome. The probes contain surrogates of ϵ‐N‐acyllysine by introduction of either hydrazide or thioamide functionalities to circumvent hydrolysis of the modification during the experiments. In addition to the modified PTMs, the developed chemotypes were analyzed with respect to the effect of peptide sequence. The photo cross‐linking conditions and subsequent functionalization of the covalent adducts were systematically optimized by applying fluorophore labeling and gel electrophoresis (in‐gel fluorescence measurements). Finally, selected probes, containing the ϵ‐N‐glutaryllysine and ϵ‐N‐myristoyllysine analogues, were successfully applied for the enrichment of native, endogenous proteins from cell lysate, recapitulating the expected interactions of SIRT5 and SIRT2, respectively. Interestingly, the latter mentioned was able to pull down two different splice variants of SIRT2, which has not been achieved with a covalent probe before. Based on this elaborate proof‐of‐concept study, we expect that the technology will have broad future applications for pairing of novel PTMs with the proteins that target them in the cell.
Hydrogen Atom Transfer‐Mediated Domino Cyclisation Reaction to Access (Spiro)Quinazolinones
Authors: Turner Oliver J. , Hirst David J. , Murphy John A. 
Published: January 2020

AbstractA radical domino cyclisation reaction of N‐cyanamide alkenes, mediated by hydrogen atom transfer (HAT) has been developed. This method, using PhSiH3 and catalytic Fe(acac)3, allows for the synthesis of challenging (spiro)quinazolinone scaffolds from simple, tractable (hetero)aryl carboxylic acid and cyanamide building blocks.