bluuebunny/crossref_metadata_2025_split huggingface dataset:
How antibiotics are used in pig farming: a mixed-methods study of pig farmers, feed mills and veterinarians in Thailand
Authors: Lekagul Angkana , Tangcharoensathien Viroj , Mills Anne , Rushton Jonathan , Yeung Shunmay 
Published: February 2020

BackgroundRising global concern about antimicrobial resistance has drawn attention to the use of antibiotics in livestock. Understanding the current usage of antibiotics in these animals is essential for effective interventions on the optimisation of antibiotic use. However, to date few studies have been conducted in low- and middle-income countries. This study aimed to explore the use of antibiotics and estimate the total amount of antibiotics used in pig production in Thailand.MethodsThis was a mixed-methods study including a cross-sectional questionnaire-based survey of 84 pig farmers, secondary analysis of data from a survey of 31 feed mills to estimate the amount of antibiotics mixed in pig feed and interviews with five veterinarians involved in the feed mill industry to gain an understanding of medicated feed production.FindingsHalf of the farmers reported using antibiotics for disease prevention. Use was significantly associated with farmers’ experience in raising pigs, farm income, having received advice on animal health and belonging to a farm cooperative. The estimated total amount of active ingredients mixed into medicated feed for pigs for the whole country was 843 tonnes in 2017. Amoxicillin was the most commonly used antibiotic reported by both pig farms and feed mills. The use of Critically Important Antimicrobials including colistin was common, with one-third of farmers reporting their use as oral or as injectable medication, and accounting for nearly two-thirds of antibiotics contained in medicated feed.ConclusionA majority of antibiotics used in Thai pig farms belonged to the category of Critically Important Antimicrobials. Progressive restriction in the use of antibiotics in pigs is recommended through using prescriptions to control the distribution of certain antibiotics. The government should strengthen veterinary services to improve access of farmers to animal health advice and explore alternative interventions.
The differential impact of economic recessions on health systems in middle-income settings: a comparative case study of unequal states in Brazil
Authors: Andrietta Lucas Salvador , Levi Maria Luiza , Scheffer Mário C , Alves Maria Teresa Seabra Soares de Britt , Carneiro Alves de Oliveira Bruno Luciano , Russo Giuliano 
Published: February 2020

IntroductionAlthough economic crises are common in low/middle-income countries (LMICs), the evidence of their impact on health systems is still scant. We conducted a comparative case study of Maranhão and São Paulo, two unevenly developed states in Brazil, to explore the health financing and system performance changes brought in by its 2014–2015 economic recession.MethodsDrawing from economic and health system research literature, we designed a conceptual framework exploring the links between macroeconomic factors, labour markets, demand and supply of health services and system performance. We used data from the National Health Accounts and National Household Sample Survey to examine changes in Brazil’s health spending over the 2010–2018 period. Data from the National Agency of Supplementary Health database and the public health budget information system were employed to compare and contrast health financing and system performance of São Paulo and Maranhão.ResultsOur analysis shows that Brazil’s macroeconomic conditions deteriorated across the board after 2015–2016, with São Paulo’s economy experiencing a wider setback than Maranhão’s. We showed how public health expenditures flattened, while private health insurance expenditures increased due to the recession. Public financing patterns differed across the two states, as health funding in Maranhão continued to grow after the crisis years, as it was propped up by transfers to local governments. While public sector staff and beds per capita in Maranhão were not affected by the crisis, a decrease in public physicians was observed in São Paulo.ConclusionOur case study suggests that in a complex heterogeneous system, economic recessions reverberate unequally across its parts, as the effects are mediated by private spending, structure of the market and adjustments in public financing. Policies aimed at mitigating the effects of recessions in LMICs will need to take such differences into account.
Impact of a pilot NHS-funded sore throat test and treat service in community pharmacies on provision and quality of patient care
Authors: Mantzourani Efi , Evans Andrew , Cannings-John Rebecca , Ahmed Haroon , Hood Kerenza , Reid Nicholas , Howe Robin , Williams Emma , Way Cheryl 
Published: February 2020

ObjectiveA National Health Service (NHS)-funded sore throat test and treat (STTT) service was introduced in selected pharmacies in two local health boards in Wales, as an extension to the national pharmacy common ailment scheme. The aim of this study was to evaluate the impact of STTT on provision and quality of patient care, namely antibiotic use, patient safety and general practitioner (GP) consultation rates.MethodsSecondary analyses of STTT consultation data to describe service outcomes, and routine data to explore changes in antibiotic prescribing and the prevalence of complications. Data were also collected from one GP practice to explore the feasibility of measuring changes in sore throat consultation rates in general practice.ResultsLess than 20% of 1725 consultations resulted in antibiotic supply. The availability of STTT was associated with greater reductions in prescriptions for phenoxymethylpenicillin than in areas where STTT was not available (−3.8% and −3.4%, difference 0.4%). When pharmacy supplies were included, the reductions in the supply of the antibiotic were similar. No increase in the monthly number of incidents of quinsy was detected, and patients were appropriately referred to other healthcare professionals during pharmacy consultations. GP consultation rates since introduction of STTT were found to be lower than the equivalent monthly average since 2014.ConclusionsData from the first 5 months of the STTT service suggest that it may have a role in safely rebalancing uncomplicated sore throat management from general practice to community pharmacies while continuing to promote antibiotic stewardship.
Mitochondrial impairment drives intestinal stem cell transition into dysfunctional Paneth cells predicting Crohn’s disease recurrence
Authors: Khaloian Sevana , Rath Eva , Hammoudi Nassim , Gleisinger Elisabeth , Blutke Andreas , Giesbertz Pieter , Berger Emanuel , Metwaly Amira , Waldschmitt Nadine , Allez Matthieu , Haller Dirk 
Published: February 2020

ObjectiveReduced Paneth cell (PC) numbers are observed in inflammatory bowel diseases and impaired PC function contributes to the ileal pathogenesis of Crohn’s disease (CD). PCs reside in proximity to Lgr5+ intestinal stem cells (ISC) and mitochondria are critical for ISC-renewal and differentiation. Here, we characterise ISC and PC appearance under inflammatory conditions and describe the role of mitochondrial function for ISC niche-maintenance.DesignIleal tissue samples from patients with CD, mouse models for mitochondrial dysfunction (Hsp60Δ/ΔISC) and CD-like ileitis (TNFΔARE), and intestinal organoids were used to characterise PCs and ISCs in relation to mitochondrial function.ResultsIn patients with CD and TNFΔARE mice, inflammation correlated with reduced numbers of Lysozyme-positive granules in PCs and decreased Lgr5 expression in crypt regions. Disease-associated changes in PC and ISC appearance persisted in non-inflamed tissue regions of patients with CD and predicted the risk of disease recurrence after surgical resection. ISC-specific deletion of Hsp60 and inhibition of mitochondrial respiration linked mitochondrial function to the aberrant PC phenotype. Consistent with reduced stemness in vivo, crypts from inflamed TNFΔARE mice fail to grow into organoids ex vivo. Dichloroacetate-mediated inhibition of glycolysis, forcing cells to shift to mitochondrial respiration, improved ISC niche function and rescued the ability of TNFΔARE mice-derived crypts to form organoids.ConclusionWe provide evidence that inflammation-associated mitochondrial dysfunction in the intestinal epithelium triggers a metabolic imbalance, causing reduced stemness and acquisition of a dysfunctional PC phenotype. Blocking glycolysis might be a novel drug target to antagonise PC dysfunction in the pathogenesis of CD.
Bile acid composition regulates GPR119-dependent intestinal lipid sensing and food intake regulation in mice
Authors: Higuchi Sei , Ahmad Tiara R , Argueta Donovan A , Perez Pedro A , Zhao Chen , Schwartz Gary J , DiPatrizio Nicholas V , Haeusler Rebecca A 
Published: February 2020

ObjectivesLipid mediators in the GI tract regulate satiation and satiety. Bile acids (BAs) regulate the absorption and metabolism of dietary lipid in the intestine, but their effects on lipid-regulated satiation and satiety are completely unknown. Investigating this is challenging because introducing excessive BAs or eliminating BAs strongly impacts GI functions. We used a mouse model (Cyp8b1–/–mice) with normal total BA levels, but alterations in the composition of the BA pool that impact multiple aspects of intestinal lipid metabolism. We tested two hypotheses: BAs affect food intake by (1) regulating production of the bioactive lipid oleoylethanolamide (OEA), which enhances satiety; or (2) regulating the quantity and localisation of hydrolysed fat in small intestine, which controls gastric emptying and satiation.DesignWe evaluated OEA levels, gastric emptying and food intake in wild-type and Cyp8b1–/–mice. We assessed the role of the fat receptor GPR119 in these effects using Gpr119–/–mice.ResultsCyp8b1–/–mice on a chow diet showed mild hypophagia. Jejunal OEA production was blunted in Cyp8b1–/–mice, thus these data do not support a role for this pathway in the hypophagia of Cyp8b1–/–mice. On the other hand, Cyp8b1 deficiency decreased gastric emptying, and this was dependent on dietary fat. GPR119 deficiency normalised the gastric emptying, gut hormone levels, food intake and body weight of Cyp8b1–/–mice.ConclusionBAs regulate gastric emptying and satiation by determining fat-dependent GPR119 activity in distal intestine.
Association of increased duration of legislated paid maternity leave with childhood diarrhoea prevalence in low-income and middle-income countries: difference-in-differences analysis
Authors: Chai Yan , Nandi Arijit , Heymann Jody 
Published: February 2020

Background
Diarrhoea is the second-leading infectious cause of death in children younger than age 5 years. The global burden of severe diarrhoeal disease is concentrated in Africa and Southeast Asia, where a significant percentage of the population resides in low-resource settings. We aimed to quantitatively examine whether extending the duration of legislated paid maternity leave affected the prevalence of childhood diarrhoea in low-income and middle-income countries (LMICs).

Methods
We merged longitudinal data measuring national maternity leave policies with information on the prevalence of bloody diarrhoea related to 884 517 live births occurring between 1996 and 2014 in 40 LMICs that participated at least twice in the Demographic and Health Surveys between 2000 and 2015. We used a difference-in-differences approach to compare changes in the percentage of children with bloody diarrhoea across eight countries that lengthened their paid maternity leave policy between 1995 and 2013 to the 32 countries that did not.

Results

The prevalence of bloody diarrhoea in the past 2 weeks was 168 (SD=40) per 10 000 children under 5 years in countries that changed their policies and 136 (SD=15) in countries that did not. A 1-month increase in the legislated duration of paid maternity leave was associated with 61 fewer cases of bloody diarrhoea (95% CI −98.86 to
−
22.86) per 10 000 children under 5 years of age, representing a 36% relative reduction.

Conclusion
Extending the duration of paid maternity leave policy appears to reduce the prevalence of bloody diarrhoea in children under 5 years of age in LMICs.
Novel, genetically induced mouse model that recapitulates the histological morphology and immunosuppressive tumor microenvironment of metastatic peritoneal carcinomatosis
Authors: Tseng Ssu-Hsueh , Park Sung-Taek , Lam Brandon , Tsai Ya-Chea , Cheng Max A , Farmer Emily , Xing Deyin , Hung Chien-Fu 
Published: February 2020

BackgroundPeritoneal carcinomatosis is a hallmark of advanced peritoneal tumor progression, particularly for tubal/ovarian high-grade serous carcinomas (HGSCs). Patients with peritoneal carcinomatosis have poor survival rates and are difficult to treat clinically due to widespread tumor dissemination in the peritoneal cavity.MethodsWe developed a clinically relevant, genetically induced, peritoneal carcinomatosis model that recapitulates the histological morphology and immunosuppressive state of the tumor microenvironment of metastatic peritoneal HGSCs by intraperitoneally injecting shp53, AKT, c-Myc, luciferase and sleeping beauty transposase, followed by electroporation (EP) in the peritoneal cavity of immunocompetent mice (intraperitoneal (IP)/EP mice).ResultsSimilar to the spread of human ovarian cancers, IP/EP mice displayed multiple tumor nodules attached to the surface of the abdomen. Histopathological analysis indicated that these tumors were epithelial in origin. These IP/EP mice also displayed a loss of CD3+T cell infiltration in tumors, highly expressed inhibitory checkpoint molecules in tumor-infiltrating and global CD4+and CD8+T cells, and increased levels of transforming growth factor-β in the ascites, all of which contribute to the promotion of tumor growth.ConclusionsOverall, our tumor model recapitulates clinical peritoneal HGSC metastasis, which makes it ideal for preclinical drug screening, testing of immunotherapy-based therapeutics and studying of the tumor biology of peritoneal carcinomatosis.
Support from superiors reduces depression in Republic of Korea military officers
Authors: Woo Seon Yeong , Kim H J , Kim B R , Ahn H C , Jang B N , Park E-C 
Published: February 2020

BackgroundThe prevalence of depression is relatively high in the Korean military. Social support is a protective factor against depression and is classified into four categories: emotional support—having the sense of feeling loved; instrumental support—receiving material assistance; informational support—receiving advice; appraisal support—feeling valued and respected for one's abilities.ObjectiveTo investigate the effect of support from one’s superior on depression among Republic of Korea (ROK) military officers.Methods2047 participants from the 2015 Military Health Survey were included in the study. The Korean version of the Beck Depression Inventory was used to measure depression, and a self-reported questionnaire was used to assess support from one’s superior. A chi-squared test and multiple logistic regression were used to analyse the data.ResultsOf the 2047 participants, 177 (8.6%) had depression. Military officers who did not receive support from their superior were more likely to have depression than than those who did receive support (OR=2.09, 95% CI 1.30 to 3.36). Additionally, military personnel who did not receive emotional or appraisal support were more likely to have depression (emotional support: OR=2.37, 95% CI 1.31 to 4.29; appraisal support: OR=1.56, 95% CI 1.48 to 2.75).ConclusionsOur study found that depression in military officers was associated with lack of support from superiors. In particular, emotional support and appraisal support had a statistically significant effect. Therefore, we suggest that the ROK armed forces consider early intervention and management for high-risk groups. A social support programme and organisational atmosphere are also needed to improve supportive ability and skills of superiors.
Testing dark matter distributions by neutrino–dark matter interactions
Authors: Penacchioni A.V. , Civitarese O. , Argüelles C. R. 
Published: February 2020

AbstractAt present, a variety of dark matter (DM) density profiles are available in the literature, able to fit the observed rotation velocity curves in galaxies. These distributions may be classified according to nature and mass of the DM candidate, and their estimation of the concentration of DM on halo scales, as well as through their central regions. Examples of these distributions are the (empiric) Einasto or isotropic, the (N-body-simulation-based) Navarro–Frenk–White (NFW), or the (elementary-particle-based) Ruffini–Argüelles–Rueda (RAR), among others. In this work, we calculate the cross-sections for the interaction between neutrinos coming from astrophysical sources and DM particles subject to some of these distributions. Furthermore, we explore the changes in the neutrino oscillation patterns due to the different DM profiles used to represent the environment. From the results of the calculations it is concluded that both the cross-section and the neutrino oscillation pattern depend on the features of each of the adopted models for the DM distributions. For the case of the NFW and isotropic DM distributions the expected effects at Earth, for neutrinos coming from an extragalactic source, are noticeable while in the case of the RAR distribution the effects are restricted to a narrow region around the galactic center.
Why $$\alpha _s$$ cannot be determined from hadronic processes without simultaneously determining the parton distributions
Authors: Forte Stefano , Kassabov Zahari 
Published: February 2020

AbstractWe show that any determination of the strong coupling $$\alpha _s$$αs from a process which depends on parton distributions, such as hadronic processes or deep-inelastic scattering, generally does not lead to a correct result unless the parton distributions (PDFs) are determined simultaneously along with $$\alpha _s$$αs. We establish the result by first showing an explicit example, and then arguing that the example is representative of a generic situation which we explain using models for the shape of equal $$\chi ^2$$χ2 contours in the joint space of $$\alpha _s$$αs and the PDF parameters.
Ginkgo biloba Induces Thrombomodulin Expression and Tissue-Type Plasminogen Activator Secretion via the Activation of Krüppel-Like Factor 2 within Endothelial Cells
Authors: Chiu Yi-Lin , Tsai Wei-Che , Wu Chih-Hsien , Wu Chun-Hsien , Cheng Cheng-Chung , Lin Wei-Shing , Tsai Tsung-Neng , Wu Lian-Shan 
Published: February 2020

The effects of thrombo-prevention, such as antiplatelet and anticoagulant activity, have been reported with the usage of Ginkgo biloba extract (GbE); however, the detailed mechanism has not yet been fully investigated, especially the role of Krüppel-like factor 2 (KLF2). This study aimed to investigate whether GbE can activate KLF2 and then induce thrombomodulin (TM) and tissue-type plasminogen activator (t-PA) secretion to enhance the effects of thrombo-prevention. Different concentrations of GbE were incubated with human umbilical vein endothelial cells (HUVECs) to evaluate its effect on endothelial cells. We found that KLF2 expression is correlated to the risk of atherosclerosis and venous thromboembolism in clinical practice. In the HUVEC cell model, GbE stimulated the expression of KLF2 in a dose-dependent manner. Moreover, TM and t-PA secretion increased when the cells were cultured with GbE. Both the expressions and activities of TM and t-PA in the GbE-treated cells declined after KLF2 was blocked by shKLF2. In sum, with GbE treatment, KLF2 expression in human endothelial cells was significantly activated, which in turn induced an increase in the protein expression and activity of TM and t-PA. After shRNA inhibited the KLF2 expression, GbE stopped inducing the expression and activity of TM and t-PA. These findings suggest that GbE exerts an antithrombotic effect on endothelial cells by increasing the TM expression and t-PA secretion; further, KLF2 is a key factor in this mechanism.
Probing the Glashow resonance at electron–positron colliders
Authors: Alikhanov I. 
Published: February 2020

The Glashow resonance is a rapid enhancement of the cross-section for scattering of electron antineutrinos on electrons at the [Formula: see text] boson production threshold due to the [Formula: see text]-channel contribution. This resonance is being searched for at large volume neutrino detectors in which the reaction [Formula: see text] to be initiated by cosmic ray antineutrinos of energies about 6.3 PeV. The relatively small neutrino flux reaching the Earth together with the necessity of analyzing ultra-high-energy final states make such searches challenging. We argue here that the Glashow resonance may contribute to the process [Formula: see text]. By extending the method of the effective (equivalent) particles to the neutral leptons, we relate the distribution of the effective neutrinos in the electron to the total cross-section for [Formula: see text]. Our approach gives a good fit to the existing experimental data measured at the collider LEP at CERN and allows one to interpret these measurements as an observation of the Glashow resonance. We also discuss an advantage of future electron–positron colliders for probing the resonance.
The sedimentology and tsunamigenic potential of the Byron submarine landslide off New South Wales, Australia
Authors: Mollison Kendall C. , Power Hannah E. , Clarke Samantha L. , Baxter Alan T. , Lane Emily M. , Hubble Thomas C. T. 
Published: February 2020

Abstract

Extensive evidence for submarine landslide failure is found along the east Australian continental margin. This paper assesses the sedimentological properties and models the failure event that created the Byron landslide scar, located on the SE Australian continental margin,
c.
34 km off the coast of Byron Bay, New South Wales. Sedimentological analyses and dating (radiocarbon and biostratigraphic) were conducted on three gravity cores collected from within the Byron landslide scar. A paraconformity, identified in one of the three cores by a distinct colour change, was found to represent a distinct radiocarbon age gap of at least 25 ka and probably represents the detachment surface of the slide plane. The core-derived sediment properties for the Byron landslide scar were used to inform hydrodynamic modelling using the freely available numerical modelling software, Basilisk. Model results highlight the important role of sediment rheology on the tsunamigenic potential of the slide and on the resulting inundation along the east Australian coastline, therefore providing a greater understanding of the modern hazard posed by comparable future submarine landslide events for the east Australian coastline.