bluuebunny/crossref_metadata_2025_split huggingface dataset:
MYC-regulated pseudogene HMGA1P6 promotes ovarian cancer malignancy via augmenting the oncogenic HMGA1/2
Authors: Tian Xiaoxue , Song Jianping , Zhang Xiyu , Yan Mingyao , Wang Shourong , Wang Yuqiong , Xu Limei , Zhao Ling , Wei Jian-jun , Shao Changshun , Kong Beihua , Liu Zhaojian 
Published: March 2020

AbstractPseudogenes have long been considered as nonfunctional genomic sequences. Recent studies have shown that they can potentially regulate the expression of protein-coding genes and are dysregulated in diseases including cancer. However, the potential roles of pseudogenes in ovarian cancer have not been well studied. Here we characterized the pseudogene expression profile in HGSOC (high-grade serous ovarian carcinoma) by microarray. We identified 577 dysregulated pseudogenes and most of them were up-regulated (538 of 577). HMGA1P6 (High mobility group AT-hook 1 pseudogene 6) was one of the overexpressed pseudogenes and its expression was inversely correlated with patient survival. Mechanistically, HMGA1P6 promoted ovarian cancer cell malignancy by acting as a ceRNA (competitive endogenous RNA) that led to enhanced HMGA1 and HMGA2 expression. Importantly, HMGA1P6 was transcriptionally activated by oncogene MYC in ovarian cancer. Our findings reveal that MYC may contribute to oncogenesis through transcriptional regulation of pseudogene HMGA1P6 in ovarian cancer.
Meningeal lymphatic vessels regulate brain tumor drainage and immunity
Authors: Hu Xueting , Deng Qiuping , Ma Lu , Li Qingqing , Chen Yidong , Liao Yuhan , Zhou Fan , Zhang Chen , Shao Linlin , Feng Jun , He Tubao , Ning Weihai , Kong Yan , Huo Yingqing , He Aibin , Liu Bing , Zhang Jingjing , Adams Ralf , He Yulong , Tang Fuchou , Bian Xiuwu , Luo Jincai 
Published: March 2020

AbstractRecent studies have shown that meningeal lymphatic vessels (MLVs), which are located both dorsally and basally beneath the skull, provide a route for draining macromolecules and trafficking immune cells from the central nervous system (CNS) into cervical lymph nodes (CLNs), and thus represent a potential therapeutic target for treating neurodegenerative and neuroinflammatory diseases. However, the roles of MLVs in brain tumor drainage and immunity remain unexplored. Here we show that dorsal MLVs undergo extensive remodeling in mice with intracranial gliomas or metastatic melanomas. RNA-seq analysis of MLV endothelial cells revealed changes in the gene sets involved in lymphatic remodeling, fluid drainage, as well as inflammatory and immunological responses. Disruption of dorsal MLVs alone impaired intratumor fluid drainage and the dissemination of brain tumor cells to deep CLNs (dCLNs). Notably, the dendritic cell (DC) trafficking from intracranial tumor tissues to dCLNs decreased in mice with defective dorsal MLVs, and increased in mice with enhanced dorsal meningeal lymphangiogenesis. Strikingly, disruption of dorsal MLVs alone, without affecting basal MLVs or nasal LVs, significantly reduced the efficacy of combined anti-PD-1/CTLA-4 checkpoint therapy in striatal tumor models. Furthermore, mice bearing tumors overexpressing VEGF-C displayed a better response to anti-PD-1/CTLA-4 combination therapy, and this was abolished by CCL21/CCR7 blockade, suggesting that VEGF-C potentiates checkpoint therapy via the CCL21/CCR7 pathway. Together, the results of our study not only demonstrate the functional aspects of MLVs as classic lymphatic vasculature, but also highlight that they are essential in generating an efficient immune response against brain tumors.
245 MHz bandwidth organic light-emitting diodes used in a gigabit optical wireless data link
Authors: Yoshida Kou , Manousiadis Pavlos P. , Bian Rui , Chen Zhe , Murawski Caroline , Gather Malte C. , Haas Harald , Turnbull Graham A. , Samuel Ifor D. W. 
Published: March 2020

AbstractOrganic optoelectronic devices combine high-performance, simple fabrication and distinctive form factors. They are widely integrated in smart devices and wearables as flexible, high pixel density organic light emitting diode (OLED) displays, and may be scaled to large area by roll-to-roll printing for lightweight solar power systems. Exceptionally thin and flexible organic devices may enable future integrated bioelectronics and security features. However, as a result of their low charge mobility, these are generally thought to be slow devices with microsecond response times, thereby limiting their full scope of potential applications. By investigating the factors limiting their bandwidth and overcoming them, we demonstrate here exceptionally fast OLEDs with bandwidths in the hundreds of MHz range. This opens up a wide range of potential applications in spectroscopy, communications, sensing and optical ranging. As an illustration of this, we have demonstrated visible light communication using OLEDs with data rates exceeding 1 gigabit per second.
Very high resolution, altitude-corrected, TMPA-based monthly satellite precipitation product over the CONUS
Authors: Hashemi Hossein , Fayne Jessica , Lakshmi Venkat , Huffman George J. 
Published: March 2020

AbstractThe Tropical Rainfall Measuring Mission (TRMM) Multisatellite Precipitation Analysis (TMPA) product provided over 17 years of gridded precipitation datasets. However, the accuracy and spatial resolution of TMPA limits the applicability in hydrometeorological applications. We present a dataset that enhances the accuracy and spatial resolution of the TMPA monthly product (3B43). We resample the TMPA data to a 1 km grid and apply a correction function derived from the Parameter-elevation Regressions on Independent Slopes Model (PRISM) to reduce bias in the data. We confirm a linear relationship between bias and elevation above 1,500 meters where TMPA underestimates measured precipitation, providing a proof-of-concept of how simple linear scaling can be used to augment existing satellite datasets. The result of the correction is the High-Resolution Altitude-Corrected Precipitation product (HRAC-Precip) for the CONUS. Using 9,200 precipitation stations from the Global Historical Climatology Network (GHCN), we compare the accuracy of TMPA 3B43 versus the new HRAC-Precip product. The results show an improvement of the mean absolute error of 12.98% on average.
Microwave radiation-induced grafting of 2-methacryloyloxyethyl trimethyl ammonium chloride onto lentil extract (LE-g-DMC) as an emerging high-performance plant-based grafted coagulant
Authors: Chua Siong Chin , Chong Fai Kait , Ul Mustafa Muhammad Raza , Mohamed Kutty Shamsul Rahman , Sujarwo Wawan , Abdul Malek Marlinda , Show Pau Loke , Ho Yeek Chia 
Published: March 2020

AbstractThe importance of graft copolymerization in the field of polymer science is analogous to the importance of alloying in the field of metals. This is attribute to the ability of the grafting method to regulate the properties of polymer ‘tailor-made’ according to specific needs. This paper described a novel plant-based coagulant, LE-g-DMC that synthesized through grafting of 2-methacryloyloxyethyl trimethyl ammonium chloride (DMC) onto the backbone of the lentil extract. The grafting process was optimized through the response surface methodology (RSM) using three-level Box-Behnken Design (BBD). Under optimum conditions, a promising grafting percentage of 120% was achieved. Besides, characterization study including SEM, zeta potential, TGA, FTIR and EDX were used to confirm the grafting of the DMC monomer chain onto the backbone of lentil extract. The grafted coagulant, LE-g-DMC outperformed lentil extract and alum in turbidity reduction and effective across a wide range of pH from pH 4 to pH 10. Besides, the use of LE-g-DMC as coagulant produced flocs with excellent settling ability (5.09 mL/g) and produced the least amount of sludge. Therefore, from an application and economic point of views, LE-g-DMC was superior to native lentil extract coagulant and commercial chemical coagulant, alum.
Mathematical model of broadly reactive plasma cell production
Authors: Erwin Samantha , Childs Lauren M. , Ciupe Stanca M. 
Published: March 2020

AbstractStrain-specific plasma cells are capable of producing neutralizing antibodies that are essential for clearance of challenging pathogens. These neutralizing antibodies also function as a main defense against disease establishment in a host. However, when a rapidly mutating pathogen infects a host, successful control of the invasion requires shifting the production of plasma cells from strain-specific to broadly reactive. In this study, we develop a mathematical model of germinal center dynamics and use it to predict the events that lead to improved breadth of the plasma cell response. We examine scenarios that lead to germinal centers that are composed of B-cells that come from a single strain-specific clone, a single broadly reactive clone or both clones. We find that the initial B-cell clonal composition, T-follicular helper cell signaling, increased rounds of productive somatic hypermutation, and B-cell selection strength are among the mechanisms differentiating between strain-specific and broadly reactive plasma cell production during infections. Understanding the contribution of these factors to emergence of breadth may assist in boosting broadly reactive plasma cells production.
Novel roles of PRK1 and PRK2 in cilia and cancer biology
Authors: Patel Hitesh , Li Jun , Herrero Ana , Kroboth Jakob , Byron Adam , Kriegsheim Alex Von , Brunton Valerie , Carragher Neil , Hurd Toby , Frame Margaret 
Published: March 2020

AbstractPRK1 and PRK2 are two closely related AGC-family serine/threonine protein kinases. Here we demonstrate novel roles for them at cilia and in cancer biology. In both instances serum withdrawal leads to increased activating PRK1 and PRK2 phosphorylation (pPRK1/pPRK2) and their depletion results in reduced spheroid growth. pPRK1/pPRK2 localise to the transition zone of cilia and their co-depletion results in reduced cilia size, impaired planer polarity and impaired cilia associated signalling. High PRK2 (but not PRK1) expression correlates with poor outcome in patients with basal-like/Triple Negative (TN) Breast Cancer (BC) where there is also higher expression relative to other BC tumour subtypes. In agreement, depletion of PRK1 and PRK2 in mouse TNBC cells, or CRISPR/Cas9 mediated deletion of PRK2 alone, significantly reduces cell proliferation and spheroid growth. Finally proteomic analysis to identify PRK2 binding partners in mouse TNBC cells revealed proteins that are important for both cilia and BC biology. Taken together these data demonstrate novel roles for PRK1 and PRK2 at cilia and in BC biology and in the case of PRK2 in particular, identifies it as a novel TNBC therapeutic target.
Phylogenomics of Rhodocyclales and its distribution in wastewater treatment systems
Authors: Wang Zhongjie , Li Wenqing , Li Hao , Zheng Wei , Guo Feng 
Published: March 2020

AbstractRhodocyclales is an abundant bacterial order in wastewater treatment systems and putatively plays key roles in multiple functions. Its phylogenomics, prevalence of denitrifying genes in sub-lineages and distribution in wastewater treatment plants (WWTPs) worldwide have not been well characterized. In the present study, we collected 78 Rhodocyclales genomes, including 17 from type strains, non-type strains and genome bins contributed by this study. Phylogenomics indicated that the order could be divided into five family-level lineages. With only a few exceptions (mostly in Rhodocyclaceae), nirS-containing genomes in this order usually contained the downstream genes of norB and nosZ. Multicopy of denitrifying genes occurred frequently and events of within-order horizontal transfer of denitrifying genes were phylogenetically deduced. The distribution of Rhodocyclaceae, Zoogloeaceae and Azonexaceae in global WWTPs were significantly governed by temperature, mixed liquor suspended solids, etc. Metagenomic survey showed that the order generally ranked at the top or second for different denitrifying genes in wastewater treatment systems. Our results provided comprehensive genomic insights into the phylogeny and features of denitrifying genes of Rhodocyclales. Its contribution to the denitrifying gene pool in WWTPs was proved.
Caveats on the use of rotenone to estimate mixotrophic grazing in the oceans
Authors: Ferreira Guilherme D. , Calbet Albert 
Published: March 2020

AbstractPhagotrophic mixotrophs (mixoplankton) are now widely recognised as important members of food webs, but their role in the functioning of food webs is not yet fully understood. This is due to the lack of a well-established technique to estimate mixotrophic grazing. An immediate step in this direction would be the development of a method that separates mixotrophic from heterotrophic grazing that can be routinely incorporated into the common techniques used to measure microplankton herbivory (e.g., the dilution technique). This idea was explored by the addition of rotenone, an inhibitor of the respiratory electron chain that has been widely used to selectively eliminate metazoans, both in the field and in the laboratory. Accordingly, rotenone was added to auto-, mixo-, and heterotrophic protist cultures in increasing concentrations (ca. 24 h). The results showed that mixotrophs survived better than heterotrophs at low concentrations of rotenone. Nevertheless, their predation was more affected, rendering rotenone unusable as a heterotrophic grazing deterrent. Additionally, it was found that rotenone had a differential effect depending on the growth phase of an autotrophic culture. Altogether, these results suggest that previous uses of rotenone in the field may have disrupted the planktonic food web.
Applicability of common inflammatory markers in diagnosing infections in early period after liver transplantation in intensive care setting
Authors: Figiel Wojciech , Grąt Michał , Niewiński Grzegorz , Patkowski Waldemar , Zieniewicz Krzysztof 
Published: March 2020

AbstractInfections remain an important cause of morbidity and mortality early after liver transplantation. The aim of this prospective longitudinal study was to evaluate clinical utility of c-reactive protein (CRP), procalcitonin, and neutrophil-to-lymphocyte ratio (NLR) in surveillance of infections early after liver transplantation in intensive care setting. A total of 60 liver transplant recipients were included. CRP, procalcitonin, and NLR assessed at 12-hour intervals were primary variables of interest. Infections and severe complications during postoperative intensive care unit stay were the primary and secondary end-points, respectively. Infections and severe complications were diagnosed in 9 and 17 patients, respectively. Only peak CRP beyond first 48 hours was associated with infections (p = 0.038) with AUC, positive and negative predictive value of 0.728, 42.9% and 92.2%, respectively (cut-off: 142.7 mg/L). Peak procalcitonin over first 60 hours was the earliest predictor (p = 0.050) of severe complications with AUC, positive and negative predictive value of 0.640, 53.3% and 80.0%, respectively (cut-off: 42.8 ng/mL). In conclusion, while CRP, procalcitonin, and NLR cannot be used for accurate diagnosis of infections immediately after liver transplantation, peak CRP beyond 48 hours and peak procalcitonin over first 60 hours may be used for initial exclusion of infections and prediction of severe complications, respectively.
Combined detection of miR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 for screening of early heart failure diseases
Authors: Ding Han , Wang Yin , Hu Longgang , Xue Sheng , Wang Yu , Zhang Lei , Zhang Yuan , Qi Hongzhao , Yu Hua , Aung Lynn Htet Htet , An Yi , Li Peifeng 
Published: March 2020

AbstractThe use of circulating microRNAs as biomarkers opens up new opportunities for the diagnosis of cardiovascular diseases because of their specific expression profiles. The aim of the present study was to identify circulating microRNAs in human plasma as potential biomarkers of heart failure and related diseases. We used real-time quantitative PCR to screen microRNA in plasma samples from 62 normal controls and 62 heart failure samples. We found that circulating miR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 expressed differently between healthy controls and heart failure patients. Plasma levels of miR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 were unaffected by hemolysis. Correlation analysis showed any two of these miRNAs possess a strong correlation, indicating a possibility of combined analysis. MiR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 could be combined in two or three or more combinations. The results suggest that miR-21-5p, miR-30a-3p, miR-30a-5p, miR-155-5p, miR-216a and miR-217 may be a new diagnostic biomarker for heart failure and related diseases.
Knockdown of long non-coding RNA SOX21-AS1 attenuates amyloid-β-induced neuronal damage by sponging miR-107
Authors: Xu Wanru , Li Kai , Fan Qian , Zong Biyun , Han Ling 
Published: March 2020

Abstract
Background: Alzheimer’s disease (AD), which has no effective drugs to delay or prevent its progression, is a multifactorial complex neurodegenerative disease. Long non-coding RNA SOX21 antisense RNA1 (SOX21-AS1) is associated with the development of AD, but the underlying molecular mechanism of SOX21-AS1 in AD is still largely unclear.
Methods: To construct the AD model, SH-SY5Y and SK-N-SH cells were treated with amyloid-β1-42 (Aβ1-42). Quantitative real-time polymerase chain reaction (qRT-PCR) was executed to detect the expression of SOX21-AS1 and miRNA-107. Western blot analysis was utilized to assess the levels of phosphorylated Tau (p-Tau). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) or flow cytometry assay was employed to determine the viability and apoptosis of SH-SY5Y and SK-N-SH cells. The relationship between SOX21-AS1 and miRNA-107 was verified with the dual-luciferase reporter assay.
Results: SOX21-AS1 expression was augmented while miR-107 expression was decreased in Aβ1-42-treated SH-SY5Y and SK-N-SH cells. Moreover, Aβ1-42 elevated the levels of p-Tau and impeded viability and induced apoptosis of SH-SY5Y and SK-N-SH cells. Also, SOX21-AS1 silencing attenuated Aβ1-42 mediated the levels of p-Tau, viability, and apoptosis of SH-SY5Y and SK-N-SH cells. Importantly, SOX21-AS1 acted as a sponge for miR-107 in SH-SY5Y and SK-N-SH cells. Furthermore, the increase in p-Tau levels and apoptosis and the repression of viability of Aβ1-42-treated SH-SY5Y and SK-N-SH cells mediated by miR-107 inhibition were partly recovered by SOX21-AS1 depletion.
Conclusion: SOX21-AS1 silencing could attenuate Aβ1-42-induced neuronal damage by sponging miR-107, which provided a possible strategy for the treatment of AD.
Ginkgo leaf extract and dipyridamole injection for chronic cor pulmonale: a PRISMA-compliant meta-analysis of randomized controlled trials
Authors: Qiu Jian , Guo Yijun , Xu Xin , Yue Hongmei , Yang Yapei 
Published: March 2020

Abstract
Ginkgo leaf extract and dipyridamole injection (GLED), a kind of Chinese herbal medicine preparation, has been considered as a promising supplementary treatment for chronic cor pulmonale (CCP). Although an analysis of the published literature has been performed, the exact effects and safety of GLED have yet to be systematically investigated. Therefore, a wide-ranging systematic search of electronic databases from which to draw conclusions was conducted. All randomized controlled trials concerning the GLED plus conventional treatments for CCP were selected in the present study. Main outcomes were treatment efficacy, blood gas and hemorrheology indexes, and adverse events. Data from 28 trials with 2457 CCP patients were analyzed. The results indicated that, compared with conventional treatments alone, the combination of conventional treatments with GLED obviously improved the markedly effective rate (RR = 1.44, 95% CI = 1.31–1.58, P &lt; 0.00001) and total effective rate (RR = 1.28, 95% CI = 1.18–1.38, P &lt; 0.00001). Moreover, the hemorrheology (PaO2, P &lt; 0.00001; PaCO2, P &lt; 0.00001; SaO2, P &lt; 0.00001; pH value, P = 0.05) and blood gas indexes (PV, WBHSV, WBMSV, WBLSV, hematocrit and FBG, P &lt; 0.01) of CCP patients were also significantly ameliorated after the combined therapy. The frequency of adverse events did not differ significantly between the two groups (P &gt; 0.05). In summary, evidence from the meta-analysis suggested that the combination of conventional treatments and GLED appeared to be effective and relatively safe for CCP. Therefore, GLED mediated therapy could be recommended as an adjuvant treatment for CCP.
Schätzen Entscheider im Gesundheitswesen die Niederlassungssituation falsch ein?
Authors: Redaèlli Marcus , Cizmowski Maren , Vollmar Horst Christian , Tamayo Miguel , Shukri Arim , Stock Stephanie , Bödecker August Wilhelm 
Published: March 2020

Zusammenfassung
Hintergrund Ziel der Untersuchung war die Abbildung von Motiven zur hausärztlichen Niederlassung aus der aktuellen Sicht nach Niederlassung (Erwartungen erfüllt) sowie vor Niederlassung (Motivation). Zudem sollten hinderliche bzw. förderliche Faktoren einer Niederlassung identifiziert werden.
Methoden Mittels Mixed-Methods-Ansatz (Fokusgruppen, Einzelinterviews) der Zielgruppe wurden Erfahrungen, Meinungen und Motivationen zur hausärztlichen Niederlassung erfasst, anschließend wurde ein prä-getesteter Fragebogen eingesetzt. Eine Vollerhebung von 675 identifizierten Hausärzten aus der Datenbank der Kassenärztlichen Vereinigung Nordrhein wurde durchgeführt. Das Einschlusskriterium war: Niederlassung 5 Jahren vor Befragungszeitraum (09/2015). Der Fragebogen enthielt 17 Fragen zur Niederlassungsmotivation, 11 zu biografischen Aspekten.
Ergebnisse Die Anzahl der auswertbaren Datensätze betrug 437 (64,7 %). Die höchsten Zustimmungswerte, im Sinne von förderlich für die Niederlassungsmotivation, erhielten „Wegfall der Dienste im Krankenhaus“ (97,2 %) und „eigener Chef sein“ (96,2 %). Als eher hinderlich wurden „Rechte und Pflichten als Vertragsarzt“ (8,5 %) und „vertragsärztlicher Notdienst/Bereitschaftsdienst“ (22,7 %) wahrgenommen. Im Sinne der Erfüllung von Erwartungen stellten sich „Wegfall der Dienste“ (95,6 %) und „Vielfalt der zu behandelnden Altersgruppen“ (88,9 %) heraus. Insgesamt würden sich 97 %, unabhängig vom Erfüllungsgrad ihrer Wünsche, wieder niederlassen und fast 3 Viertel entschieden sich erst nach dem Studium für die hausärztliche Tätigkeit.
Schlussfolgerungen Die Tatsache, dass die Flucht aus dem stationären Sektor als Hauptantrieb zur Niederlassung zählt, muss nachdenklich stimmen. Da dieser Befragungsansatz eine Vollerhebung von hausärztlich Niedergelassenen aus einem 5-jährigen Zeitraum darstellt, kann festgestellt werden, dass aufgrund der Altersstruktur der Befragten die derzeitigen Anreizstrukturen einer Niederlassung neu überdacht werden sollten.