bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comparative Study of Vitrectomy Combined with Internal Limiting Membrane Peeling for Idiopathic Macular Hole of different hole diameters with Air or C3F8 Intraocular Tamponade
Authors: Tang Yong Ping , Wu Bei Lei , Lin Zhong , Wu Ronghan 
Published: December 2019

Abstract
Background：Vitrectomy and membrane peeling followed by gas filling technique has become a standard procedure of macular hole surgery, the outcomes are affected by many factors, and which kind of intraocular tamponade is the best choice still has some conflicts. The purpose of this study is to investigate whether air filling can achieve comparable effects to long-acting inert gases when different macular hole sizes are enrolled. Methods：116 patients with idiopathic macular hole were enrolled in this retrospective study, 44 received C3F8 tamponade and 72 received sterile air tamponade. Before and after vitrectomy surgery, the best corrected visual acuity, slit lamp examination, fundus examination and intraocular pressure were analyzed. Results：No statistically significant difference was fund in age, gender, axial length, intraocular pressure, or preoperative visual acuity between groups. After the surgery, the closure rates of air group and C3F8 group with macular hole diameter ≤400 microns were 94.4% and 100.0%, with no significant difference between the two groups (P = 0.701). The closure rates of macular hole diameters lager than 400 microns are 66.7% and 91.3%, P = 0.029. The total closure rates of the two groups are 80% and 95.6%, P =0.018. Conclusions：In idiopathic macular hole surgery, the effect of air as an intraocular tamponade material is similar to that of C3F8 in macular hole with diameters less than 400 microns. In patients with larger macular hole (diameter over 400 microns) performed therapeutic surgery with air filling, internal limiting membrane insertion or flap inversion may be recommended get better outcomes.
Family Planning for Women with Severe Mental Illness in Rural Ethiopia: A Qualitative Study
Authors: Zerihun Tigist , Sorsdahl Katherine , Hanlon Charlotte 
Published: December 2019

Abstract
Background: Family planning is a crucial issue for all women of reproductive age, but in women with severe mental illness (SMI) there may be particular challenges and concerns. As primary care-based mental health services are expanding in low- and middle-income countries (LAMICs), there is an opportunity to improve family planning services for women with SMI. However, research exploring unmet family planning needs of women with SMI in such settings is scarce. Therefore, the present study explored the family planning experiences, unmet needs and preferences of women with SMI who reside in a predominantly rural area of Ethiopia Methods: A qualitative study design was used. Women with SMI who were participating in the ongoing population-based cohort study in Butajira were selected purposively on the basis of responses to a quantitative survey of current family planning utilization. In-depth interviews were conducted with 16 women with SMI who were of reproductive age. Audio files were transcribed in Amharic, translated into English and analyzed using the Framework Approach using Open Code qualitative data analysis software. Results: Participants reported pervasive effects of SMI upon the intimate relationships and sexual life of women. Although women with SMI felt that family planning was important, they had limited knowledge of family planning generally and a lack of understanding of the specific family planning needs relevant to having SMI. None of the women with SMI in the present study had received any recommendations to use family planning services while accessing mental health care services. The participants identified ways in which primary care-based mental health services could better meet their family planning needs. Conclusion: This study has provided in-depth perspectives from women with SMI about the broader context of their family planning experience, needs, and barriers and how integrated primary care services could better meet their needs. Empowerment of women with SMI to access information and services needs to be an important focus of future efforts to improve the reproductive experiences of this vulnerable group. Key words: Family planning, severe mental illness, Contraceptives, Reproductive age
Osteoprotegerin prompts cardiomyocyte hypertrophy via FAK/BECLIN1 mediated autophagy inhibition
Authors: shen anna , Zheng Dezhong , Liu Tingrong , Zhou Tao 
Published: December 2019

Abstract
Aim It has been reported that Osteoprotegerin (OPG) induces cardiomyocyte hypertrophy, but the mechanism remains unclear. This study was to investigate the role of Focal Adhesion Kinase (FAK) pathway in the OPG induced hypertrophy in cultured cardiomyocytes.Methods The H9C2 line of rat cardiomyocytes were treated with OPG at different concentrations and the cellular hypertrophy was evaluated. Meanwhile, the activity of FAK and other the phosphorylation kinases were detected. Autophagy flux assay was performed in absence and presence OPG. The interaction between proteins was analyses using Co-Immunoprecipitation assay.Results We found that OPG induced cardiomyocyte hypertrophic response, accompanied by dramatic increases a series of inflammatory factors and cytokines, as well as collagen synthesis. Also OPG inhibits autophagy and induces FAK phosphorylation. FAK silencing using si-RNA abrogates the effect of OPG on autophagy and cellular hypertrophy. Furthermore, Co-Immunoprecipitation assay reveals that OPG inhibits autophagy through enhancing the binding of FAK and Beclin1 Tyr 233.Conclusion The FAK/Beclin1 signal pathway is essential for the OPG induced autophagy inhibition and hypertrophic response in cultured H9C2 cells.
Predictors of acute intracranial hemorrhage and recurrence of chronic subdural hematoma following burr hole drainage
Authors: Chen FuMei , Wang Ke , Xu KangLi , Wang Li , Zhan TianXiang , Cheng Fei , Wang Hao , Chen Zuo-Bing , Gao Liang , Yang Xiaofeng 
Published: December 2019

Abstract
Objective To investigate predictors of postoperative acute intracranial hemorrhage (AIH) and recurrence of chronic subdural hematoma (CSDH) after burr hole drainage.MethodsA multicenter retrospective study of patients who underwent burr hole drainage for CSDH between January 2013 and March 2019. Univariate and multiple logistic regression analyses were performed to determine the relationship between CSDH recurrence and postoperative AIH and clinical, radiological, and surgical variables. Results A total of 456 CSDH patients (361 males, 95 females) were enrolled in the study. The median (IQR) age was 68 (61–77) years (range: 8–98 years). CSDH recurrence occurred in 63 patients (56 males, seven females), with a recurrence rate of 13.8%. The median time interval between initial burr hole drainage and recurrence was 37 (27–46) days (range: 12–180 days). Postoperative AIH developed in 23 patients (15 males, eight females), with an incidence of 5.0%. The median time interval between initial burr hole drainage and postoperative AIH was 4 (2–6) days (range: 1–10 days). Bilateral hematoma, hyperdense hematoma and anticoagulant drug use were independent predictors of recurrence in the multiple logistic regression analyses. Preoperative headache was an independent risk factor of postoperative AIH in the multiple logistic regression analyses, however, intraoperative irrigation reduced the incidence of postoperative AIH. Conclusions This study found that bilateral hematoma, hyperdense hematoma and anticoagulant drug use were independently associated with CSDH recurrence. Clinical presentation of headache was the strongest predictor of postoperative AIH, and intraoperative irrigation decreased the incidence of postoperative AIH.
Country of first birth and neonatal outcomes in migrant and Norwegian-born parous women in Norway: a population-based study
Authors: Vik Eline Skirnisdottir , Nilsen Roy Miodini , Aasheim Vigdis , Small Rhonda , Moster Dag , Schytt Erica 
Published: December 2019

Abstract
Background: This study compares subsequent birth outcomes in migrant women who had already had a child before arriving in Norway with those in migrant women whose first birth occurred in Norway. The aim of this study was to investigate the associations between country of first birth and adverse neonatal outcomes (very preterm birth, moderately preterm birth, post-term birth, small for gestational age, large for gestational age, low Apgar score, stillbirth and neonatal death) in parous migrant and Norwegian-born women. Methods: National population-based study including second and subsequent singleton births in Norway from 1990-2016. Data were retrieved from the Medical Birth Registry of Norway and Statistics Norway. Neonatal outcomes were compared between births to: 1) migrant women with a first birth before immigration to Norway (n=30,062) versus those with a first birth after immigration (n=66,006), and 2) Norwegian-born women with a first birth outside Norway (n=6,205) versus those with a first birth in Norway (n=514,799). Associations were estimated as crude and adjusted odds ratios (aORs) with 95% confidence intervals (CIs) using multiple logistic regression. Results: Migrant women with a first birth before immigrating to Norway had increased odds of adverse outcomes in subsequent births relative to those with a first birth after immigration: very preterm birth (22-31 gestational weeks (gwks); aOR=1.27; CI 1.09-1.48), moderately preterm birth (32-36 gwks; aOR=1.10; CI 1.02-1.18), post-term birth (≥42 gwks; aOR=1.19; CI 1.11-1.27), low Apgar score (<7 at 5 minutes; aOR=1.27; CI 1.16-1.39) and stillbirth (aOR=1.29; CI 1.05-1.58). Similar results were found in the sample of births to Norwegian-born women. Conclusions: The increased odds of adverse neonatal outcomes for migrant and Norwegian-born women who had their first births outside Norway should serve as a reminder of the importance of taking a careful obstetric history in these parous women to ensure appropriate care for their subsequent pregnancies and births in Norway. Keywords: immigration, parous women, neonatal outcomes, obstetric history, predictor
Knowledge, attitudes, and practices of tertiary education students in regard to air pollution in Windhoek, Namibia: a cross sectional study
Authors: KEMBA HAUSHIKU , Nkwenika Tshifhiwa , Nkosi Vusumusi 
Published: December 2019

Abstract
Background: Knowing the residents' knowledge, attitude, and practices to air pollution is necessary for developing interventions specific to that population. Air pollution remains a neglected environmental concern in Windhoek, Namibia and people’s perceptions about air pollution and health have not been documented. This study investigated the knowledge, attitudes and practices of tertiary students to air pollution in Windhoek. Methods: Students from five tertiary institutions in Windhoek, Namibia were included in cross sectional study. A previously validated structured questionnaire; designed to collect information on knowledge, attitudes, and practices regarding air pollution was used to collect data from 321 randomly selected study participants.Results: More than half (59.4%) of the study participants thought that air pollution was a major problem, while over 90% knew that air pollution could negatively impact health. The media was found to be the main source of information for study participants (91.1%). Despite having the knowledge, less than a third (27%) of the respondents regularly took action out of concern of air pollution. There was a statistically significant association between knowledge, attitudes, and practices to air pollution (all P < 0.05). Conclusions: Air pollution interventions targeting tertiary students should be aimed at informing them on ways in which they could contribute individually and collectively to reducing air pollution and its negative effects.
IgE reactivity to components of fish allergens in Pacific cod (Gadus macrocephalus) in atopic dogs
Authors: Imanishi Ichiro , Uchiyama Jumpei , Mizukami Keijiro , Kamiie Junichi , Kurata Keigo , Fujimura Masato , Shimakura Kuniyoshi , Nishifuji Koji , Sakaguchi Masahiro 
Published: December 2019

Abstract
Background IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude and purified Pacific cod (Gadus macrocephalus) allergens in atopic dogs to identify the allergenic components of cod. Methods Specific IgE to crude cod allergens in the sera of 179 atopic dogs, including 27 dogs with cod allergy, were measured using enzyme-linked immunosorbent assay (ELISA). The allergenic components of crude cod antigen were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry (LC-MS/MS). IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that were positive for specific IgE to crude cod allergens. Results Specific IgE to crude cod allergens were present in 36 (20%) of the 179 atopic dogs and 12 (44%) of the 27 dogs with cod allergy. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs had specific IgE to parvalbumin, collagen, and tropomyosin, respectively. Conclusions The dogs exhibited IgE reactivity to the cod allergen components similar to that observed in humans, providing support for the use of atopic dogs with fish allergy as a model for fish allergy in humans.
The development of early ascites is associated with shorter overall survival in patients with hepatocellular carcinoma treated with drug-eluting embolic chemoembolization
Authors: Pipa-Muniz María , Sanmartino Susana , Mesa Alicia , Alvarez-Navascués Carmen , González-Diéguez Maria Luisa , Cadahía Valle , Rodríguez José Eduardo , Vega Florentino , Rodríguez Manuel , Costilla-García Serafín Marcos , Calvo Maria Varela 
Published: December 2019

Abstract
Background: A single-centre cohort study was performed to identify the independent factors associated withthe overall survival (OS) of hepatocellular carcinoma (HCC) patients treated with transarterial chemoembolization with drug-eluting beads (DEB-TACE). Methods: A total of 216 HCC patients who underwent DEB-TACE from October 2008 to October 2015 at a tertiary hospital were consecutively recruited. The analysis of prognostic factors associated with DEB-TACE complications was performed. Results: The objective response (OR) rate (Modified Response Evaluation Criteria in Solid Tumors (mRECIST) criteria) to the first DEB-TACE (DEB-TACE-1) was 70.3%; the median OS from DEB-TACE-1 was 27 months (95% confidence interval (CI), 24-30). In the multivariate analysis, basal Barcelona Clinic Liver Cancer (BCLC) stage and serum alkaline phosphatase were independent factors for survival following DEB-TACE-1. The most important clinical event associated with poor survival was the development of early ascites after DEB-TACE-1 (median OS, 17 months), which was closely related to the history of ascites, albumin and hemoglobin but not to tumour load or to response to therapy. Conclusions: Early ascites post-DEB-TACE is associated with the survival of patients despite adequate liver function and the use of a supra-selective technical approach. History of ascites, albumin and hemoglobin are major determinants of the development of early ascites post-DEB-TACE.
Effect of low-intensity ultrasound on the colonization of spermatogonial stem cells
Authors: Moghaddam Zeinab Hormozi , Mokhtari-Dizaji Manijhe , Movahedin Mansoureh 
Published: December 2019

Abstract
Background: Low-level-intensity ultrasound waves contribute to the proliferation, differentiation, and increasing the number of cells in vitro. However, the interaction of ultrasonic waves involving thermal effects, mechanical vibration, or cavitation as a cell proliferation factor has not been determined. Therefore, the aim of this study was to investigate the effect of cavitation (mechanical index) on spermatogonial stem cell proliferation and colonization by removing heat effects caused by ultrasound radiation. Isolated spermatogonial stem cells from neonatal mice were cultured in a DMEM culture medium with 10% FBS. Spermatogonial stem cells were stimulated by low-level ultrasound for five days and colonization and viability were evaluated on the 7th day.Results: Regarding the low intensity of ultrasound, the Rayleigh integral model was used for acoustic pressure computation. According to the results of modeling, the intensities of 0.28, 0.45, 0.96, and 1.34 W/cm2 were selected at 0.5 cm of distance with the mechanical indices of 0.40, 0.51, 0.75, and 0.89. The mechanical indices of 0.40 and 0.89 resulted in 93±4 and 32±4 colonies, respectively. An increase in colony diameter was observed for the mechanical index of 0.40 during all days of the culture. In the culture on the 7th day, it had the largest average colony diameter of 134.05±1.22 μm in comparison with other groups (p<0.05). Cell viability did not significantly differ across groups (p=0.08). Conclusion: The results suggest that a low-intensity ultrasound of 40 kHz with a 0.40 mechanical index can be effective in increasing the proliferation and colonization of spermatogonial stem cells during culture. In other words, a mechanical index within the threshold of cavitation increases the proliferation and colonization of spermatogonial stem cells.
Factors predictive of invasive ductal carcinoma in cases preoperatively diagnosed as ductal carcinoma in situ
Authors: Takada Koji , Kashiwagi Shinichiro , Asano Yuka , Goto Wataru , Morisaki Tamami , Takahashi Katsuyuki , Fujita Hisakazu , Takashima Tsutomu , Tomita Shuhei , Hirakawa Kosei , Ohira Masaichi 
Published: December 2019

Abstract
Background Invasion is often found in the postoperative pathological examination of cases diagnosed as ductal carcinoma in situ (DCIS) by histological examinations such as core needle biopsy (CNB) or vacuum-assisted biopsy (VAB). A meta-analysis reported that 25.9% of invasive ductal carcinoma (IDC) cases are diagnosed as DCIS preoperatively by CNB. Risk factors for invasion by postoperative examination have been studied, but no factors have been found that could be assessed preoperatively from blood tests. In this study, we investigated factors predictive of invasion based on preoperative blood tests in patients diagnosed with DCIS by preoperative biopsy. MethodsIn this study, 118 patients who were diagnosed with DCIS by preoperative biopsy were included. Biopsies were performed with 16-gauge CNB or VAB. Peripheral blood was obtained at the time of diagnosis. This study evaluated absolute platelet count, absolute lymphocyte count, lactate dehydrogenase, carcinoembryonic antigen, and cancer antigen 15-3 (CA15-3). The platelet–lymphocyte ratio (PLR) was calculated by dividing the absolute platelet count by the absolute lymphocyte count, and patients were grouped into high PLR (≥160.0) and low PLR (<160.0) groups. ResultsInvasion was found more frequently after surgery in pathologically high-grade cases than in pathologically not-high-grade cases (p = 0.015). The median PLR was 138.9, and 48 patients (40.7%) were classified into the high PLR group. The high PLR group was significantly more likely to have invasion in the postoperative pathology than the low PLR group (p = 0.018). In multivariate analysis of factors predictive of invasion in postoperative pathology, a high PLR (p = 0.007, odds ratio [OR] = 3.212), larger tumor size (p = 0.044, OR = 2.758), and biopsy method (VAB vs CNB, p = 0.001, OR = 0.206) were independent risk factors. Conclusions The PLR may be a predictor of invasion in the postoperative pathology for patients diagnosed with DCIS by preoperative biopsy.
Mineralization mechanisms of groundwater in a semi-arid area in Algeria: statistical and hydrogeochemical approaches
Authors: Bouteldjaoui F. , Bessenasse M. , Taupin J.-D. , Kettab A. 
Published: December 2019

Abstract
The study area of Ain Oussera (3,790 km2) is located in the semi-arid high plains of the Saharian Atlas (200 km south of Algiers). Groundwater investigated in the present study is from the Albian formations which are considered as a major source for drinking and irrigation water. The objective of this study is to identify the different hydrochemical processes controlling the groundwater mineralization. For this purpose, chemical analyses were performed on 31 wells sampled during May 2014. The chemical study (total dissolved solids (TDS), Piper, chemical correlation) allowed the origins of groundwater mineralization to be identified. The dissolution of evaporate minerals, precipitation of carbonate minerals, and ion exchange reactions have been identified as major sources of mineralization processes. Anthropogenic processes due to human activities (sewage efﬂuents and agricultural fertilizers) also contribute to the mineralization of the water. The results of principal component analysis also confirm that both natural and anthropogenic processes contribute to the chemical composition of groundwater in the study area.
Comparison of Vascular Responses to Vasoconstrictors in Human Placenta in Preeclampsia between Preterm and Later Term
Authors: Feng Xueqin , Zhang Yumeng , Tao Jianying , Lu Likui , Zhang Yingying , Liu Jingliu , Zhao Meng , Guo Jun , Zhu Dan , Zhu Jianguo , Xu Zhice 
Published: December 2019

Background:
Placental blood vessels play important roles in maternal-fetal circulation. Although
pathologic mechanisms of preeclampsia are unclear, it is known that placental vascular dysfunction
could contribute to pregnant hypertension. However, placental micro-vessel function or dysfunction
at preterm has not been investigated.

Methods:
Human placentas from normal and preeclamptic pregnancies at preterm and term were obtained.
Placental micro-vessels were used for determining vascular tension and responses to various
vasoconstrictors as well as intracellular calcium store capability. It was the first time to show vascular
responses in placental arteries to angiotensin II, endothelin-1, and other vascular drugs at preterm.

Results:
Compared to the control, placental vascular contractile responses to angiotensin II and caffeine
were significantly decreased, while placental vascular responses to KCl, endothelin-1, and bradykinin
were not significantly altered in the later term group in preeclampsia. In comparison of placental
micro-vessel tension between the preterm and later term, caffeine- and serotonin-induced vascular
contractions were significantly weaker in the preterm than that in the later term. On the contrary, vascular
response to angiotensin II was increased in the preterm preeclampsia, while KCl-, endothelin-1,
and bradykinin-mediated placental vessel responses in the preterm preeclampsia were similar to that in
later term preeclampsia.

Conclusion:
New data showed that micro-vessel responses to angiotensin II and serotonin, not endothelin-
1 or bradykinin, were significantly reduced in the human placentas at preterm, and intracellular
Ca2+ store capacity was damaged too, providing important information on possible contributions of
placental vascular dysfunction to pregnant hypertension.