bluuebunny/crossref_metadata_2025_split huggingface dataset:
XYLAN O-ACETYLTRANSFERASE 6 promotes xylan synthesis by forming a complex with IRX10 and governs wall formation in rice
Authors: Wen Zhao , Xu Zuopeng , Zhang Lanjun , Xue Yi , Wang Hang , Jian Lin , Ma Jianing , Liu Zhuolin , Yang Hanlei , Huang Shaohui , Kang Xue , Zhou Yihua , Zhang Baocai 
Published: December 2024

Abstract
Xylan, a pivotal polymer with diversified structures, is indispensable for cell wall integrity and contributes to plant growth and biomass recalcitrance. Xylan is synthesized by multienzyme complexes named xylan synthase complexes (XSCs). However, the biochemical mechanism of XSCs and the functions of core components within XSC remain unclear. Here, we report that rice (Oryza sativa) XYLAN O-ACETYLTRANSFERASE 6 (XOAT6) and the xylan synthase IRREGULAR XYLEM10 (IRX10) represent core components of the XSC, acting together to biosynthesize acetyl-xylans. Co-fractionation mass spectrometry and protein–protein interaction analyses revealed that IRX10 and XOAT6 physically interact within XSC, corroborated by similar xylan defects in xoat6 and irx10 mutants. Biochemical assays showed that XOAT6 is an O-acetyltransferase of the xylan backbone and facilitates chain polymerization catalyzed by IRX10. Fluorescence correlation spectroscopy further visualized the xylooligomer polymerization process at a single-molecule level. Solid-state NMR analysis, electron microscopy observations, and nanoindentation examinations identified the altered xylan conformation, disorganized cellulosic structure, and increased wall rigidity and cellulose accessibility in the mutants, leading to brittleness and improved saccharification efficiency. Our findings provide insights into the assembly of XSCs and xylan biosynthesis and offer a framework for tailoring xylans to improve crop traits and biomass.
Temperature-driven changes in membrane fluidity differentially impact FILAMENTATION TEMPERATURE-SENSITIVE H2-mediated photosystem II repair
Authors: Zhang Jingzhi , Lee Keun Pyo , Liu Yanling , Kim Chanhong 
Published: December 2024

Abstract
The Arabidopsis (Arabidopsis thaliana) yellow variegated2 (var2) mutant, lacking functional FILAMENTATION TEMPERATURE-SENSITIVE H2 (FtsH2), an ATP-dependent zinc metalloprotease, is a powerful tool for studying the photosystem II (PSII) repair process in plants. FtsH2, forming hetero-hexamers with FtsH1, FtsH5, and FtsH8, plays an indispensable role in PSII proteostasis. Although abiotic stresses like cold and heat increase chloroplast reactive oxygen species (ROS) and PSII damage, var2 mutants behave like wild-type plants under heat stress but collapse under cold stress. Our study on transgenic var2 lines expressing FtsH2 variants, defective in either substrate extraction or proteolysis, reveals that cold stress causes an increase in membrane viscosity, demanding more substrate extraction power than proteolysis by FtsH2. Overexpression of FtsH2 lacking substrate extraction activity does not rescue the cold-sensitive phenotype, while overexpression of FtsH2 lacking protease activity does in var2, with other FtsH isomers present. This indicates that FtsH2's substrate extraction activity is indispensable under cold stress when membranes become more viscous. As temperatures rise and membrane fluidity increases, substrate extraction activity from other isomers suffices, explaining the var2 mutant's heat stress resilience. These findings underscore the direct effect of membrane fluidity on the functionality of the thylakoid FtsH complex under stress. Future research should explore how membrane fluidity impacts proteostasis, potentially uncovering strategies to modulate thermosensitivity.
Cyclic di-AMP regulates genome stability and drug resistance in Mycobacterium through RecA-dependent and RecA-independent recombination
Authors: Mudgal Sudhanshu , Goyal Nisha , Kasi Manikandan , Saginela Rahul , Singhal Anusha , Nandi Soumyadeep , Mahmud A K M Firoj , Muniyappa Kalappa , Sinha Krishna Murari 
Published: December 2024

Abstract
In Escherichia coli, RecA plays a central role in the rescue of stalled replication forks, double-strand break (DSB) repair, homologous recombination (HR), and induction of the SOS response. While the RecA-dependent pathway is dominant, alternative HR pathways that function independently of RecA do exist, but relatively little is known about the underlying mechanism. Several studies have documented that a variety of proteins act as either positive or negative regulators of RecA to ensure high-fidelity HR and genomic stability. Along these lines, we previously demonstrated that the second messenger cyclic di-AMP (c-di-AMP) binds to mycobacterial RecA proteins, but not to E. coli RecA, and inhibits its DNA strand exchange activity in vitro via the disassembly of RecA nucleoprotein filaments. Herein, we demonstrate that Mycobacterium smegmatis ΔdisA cells, which lack c-di-AMP, exhibit increased DNA recombination, higher frequency of mutation, and gene duplications during RecA-dependent and RecA-independent DSB repair. We also found that c-di-AMP regulates SOS response by inhibiting RecA-mediated self-cleavage of LexA repressor and its absence enhances drug resistance in M. smegmatis ΔdisA cells. Together, our results uncover a role of c-di-AMP in the maintenance of genomic stability through modulation of DSB repair in M. smegmatis.
Cluster Configurations in Li Isotopes in the Variation of Multi-Bases of the Antisymmetrized Molecular Dynamics
Authors: Myo Takayuki , Lyu Mengjiao , Zhao Qing , Isaka Masahiro , Wan Niu , Takemoto Hiroki , Horiuchi Hisashi , Doté Akinobu 
Published: December 2024

Abstract
We investigate the cluster configurations in Li isotopes, which are described in the optimization of the multi-Slater determinants of the antisymmetrized molecular dynamics. Each Slater determinant in the superposition is determined simultaneously in the variation of the total energy. The configurations of the excited states are obtained by imposing the orthogonal condition onto the ground-state configurations. In Li isotopes, various cluster configurations are confirmed and are related to the thresholds of the corresponding cluster emissions. For $^5$Li, we predict the $^3$He+$d$ clustering in the excited state as well as the mirror state of $^5$He with $^3$H+$d$. For $^{6-9}$Li, various combinations of the clusters are obtained in the ground and excited states, and the superposition of these basis states reproduces the observed energy spectra. For $^9$Li, we predict the linear-chain states consisting of various cluster configurations at excitation energy levels of 10–13 MeV.
Theory-Informed Development of a Multicomponent Intervention to Implement Clinical Practice Guideline Recommendations in the Management of Shoulder Pain
Authors: Lowry Véronique , Desmeules François , Lavigne Patrick , Décary Simon , Tousignant-Laflamme Yannick , Martel Marylie , Roy Jean-Sébastien , Perreault Kadija , Lefebvre Marie-Claude , Kilpatrick Kelley , Hudon Anne , Zidarov Diana 
Published: December 2024

ABSTRACT

Objective
Suboptimal primary health care management of shoulder pain has been reported in previous studies. Implementing clinical practice guidelines (CPGs) recommendations using a theoretical approach is recommended to improve shoulder pain management. This study aims to identify determinants of implementing recommendations from shoulder CPGs to help develop an intervention based on the identified determinants.

Methods
Family physicians and physical therapists managing patients with shoulder pain in primary care were invited to participate in a qualitative study to identify determinants to implementing recommendations from shoulder CPGs. The Theoretical Domains Framework (TDF) was used to inform the creation of the semi-structured interview guide and for deductive coding of transcriptions. The determinants were mapped to intervention functions and behavior change techniques (BCT) using the Behavior Change Wheel method and strategies for implementing CPGs recommendations were identified.

Results
Interviews were conducted with 16 family physicians and 19 physical therapists. We identified 12 barriers and 6 facilitators within 7 TDF domains: knowledge, skills, beliefs about capabilities, beliefs about consequences, intentions, environmental context and resources, and social influence. We identified 6 intervention functions and 12 BCT addressing the relevant determinants. The 11 implementation strategies identified include the development and distribution of educational material, interactive educational outreach visits, and audit and feedback. Other components to consider are the identification and preparation of champions in primary care clinical settings, revision of professional roles, and creation of interdisciplinary clinical teams.

Conclusions
The identification of barriers and facilitators to implementing recommendations from shoulder CPGs allowed us to select implementation strategies at individual and organizational levels.

Impact
The implementation strategies will be adapted to specific primary care contexts in consultation with stakeholders and operationalized into a multicomponent implementation intervention. Implementing the intervention has the potential to improve shoulder pain management in primary care and facilitate the use of evidence-based recommendations from CPGs.
On the scanning map and the space of smooth complex projective hypersurfaces
Authors: Alonso Ángel Javier , Cantero-Morán Federico 
Published: December 2024

Abstract
The space of degree d smooth projective hypersurfaces of $\mathbb{C}P^n$ admits a scanning map to a certain space of sections. We compute a rational homotopy model of the action by conjugation of the group $U(n+1)$ on this space of sections, from which we deduce that the scanning map induces a monomorphism on cohomology when d &gt; 2. Our main technique is the rational homotopy theory of Sullivan and, more specifically, a Sullivan model for the action by conjugation of a connected topological group on a space of sections.
A deep dive into monogenic lupus: insights on DNASE1L3 mutation
Authors: Abdwani Reem , Mal Mahadev Jetho , Al Masroori Eman , Jaju Sanjay , Al Abrawi Safiya 
Published: December 2024

Abstract

Objective
In this study, we aim to describe the largest cohort of monogenic lupus caused by DNASE1L3 yet reported, describing its phenotypic characteristics and outcomes.

Methods
We performed a multicentre retrospective chart review for enrolled patients with childhood-onset SLE (cSLE) followed in paediatric rheumatology tertiary centers in the Sultanate of Oman. We included cSLE patients with genetically confirmed DNASE1L3 mutation. The demographic, clinical and laboratory data of the monogenic lupus cohort was compared with sporadic cSLE cohort.

Results
We recruited 33 patients from 15 families with confirmed homozygous DNASE1L3 mutation. The median age of disease onset was 4 years, with 18 (55%) of the cohort presenting before the age of 5 years. There was a higher proportion of boys 20 (61%) affected. A significant proportion of cohort had hypocomplementemic urticarial vasculitis (HUV) symptoms including arthritis (82%), urticarial vasculitis (79%), fever (49%), abdominal pain (36%) and conjunctivitis (25%). Compared with the sporadic cohort, there was higher frequency of nephritis (60%) and pulmonary haemorrhage (15%). SLE disease activity index score on presentation was 13.3 ± 3.64 (s.d.), while disease damage was identified in n = 14 (42%) of patients with mean damage index score of 2.14 ± 1.12 (s.d.). Despite treatment, a significant proportion continued to display HUV symptoms that were refractory to standard treatment.

Conclusion
Given the early onset, aggressive nature and refractory natures of cSLE caused by DNASE1L3 mutation, further research aimed at targeted therapies that could restore the function of DNASE1L3 or compensate for its deficiency is warranted.
Central banks, monetary stability, and the varieties of capital control liberalization
Authors: Rademacher Inga 
Published: December 2024

Abstract
This article explores the differential liberalization of capital controls in advanced market economies. It shows that although finance-led economies abolished controls by the 1980s, export-led economies retained significant restrictions into the 1990s, contributing to differential financial market development. A historical comparison of Germany and the UK (1961–1985) finds that differential control use resulted from central bankers’ varying expectations of controls’ monetary functions in the domestic economic context. As capital became mobile in the 1960s, sudden capital in- and outflows jeopardized price and currency stability. In the German context of macroeconomic stability, controls effectively limited capital inflows and arrested destabilizing inflationary and exchange rate pressures. However, in the British context of macroeconomic instability and capital outflows, controls could not sufficiently restore stability: as loose fiscal and monetary choices persisted, monetary conditions deteriorated. Thus, Bank of England officials became fervent advocates for control liberalization to enforce restrictive fiscal and monetary choices via market pressures.
Impacts of birth season and production system on gastrointestinal parasitism and growth in Katahdin lambs
Authors: Murphy Thomas W , Burke Joan M , Hess Andrew S , Miller James E , Wood Erin L , Acharya Mohan 
Published: December 2024

Abstract
Gastrointestinal nematode (GIN) infection adversely affects the performance and well-being of forage-based sheep throughout the world. The study objectives were to estimate longitudinal differences between birth seasons and production systems for lamb postweaning growth and indicators of GIN infection. Data were collected on Katahdin lambs within a single flock from 2006 to 2022. Lambs were born in either the winter or fall and reared in conventional or certified organic production systems. Four sub-flocks were defined as the combination of birth season and production system (winter-conventional = W-C, n = 1766; winter-organic = W-O, n = 190; fall-conventional = F-C, n = 846; and fall-organic = F-O, n = 189). All lambs were naturally infected with GIN from previously grazed pastures, weaned at approximately 60 d of age, and remained on pasture throughout the postweaning phase. At approximately 90, 120, and 150 d of age lamb body weight (BW) was recorded, and fecal egg count and blood packed cell volume (PCV) were quantified. Fecal egg count was log-transformed (LFEC) prior to analyses. Traits were initially analyzed within collection timepoint to compare all 4 sub-flocks. Final BW at 150 d and LFEC and PCV throughout the postweaning phase were similar between conventional and organic lambs born in the same season. Repeated measures models were then used to analyze records from W-C and F-C lambs over time. The collection timepoint × deworming treatment × sub-flock interaction effect was significant for all traits (P &lt; 0.01). Within lambs that did not require deworming, no difference in BW between birth seasons was observed. However, LFEC trends for untreated F-C and W-C lambs were nearly exact opposite of one another, being greater for F-C at 90 d, (P &lt; 0.01) not different at 120 d, and greater for W-C at 150 d (P &lt; 0.01). Additionally, PCV of untreated F-C lambs was lower at 90 d but greater at 120 and 150 d than untreated W-C lambs (P ≤ 0.04). This was the first study conducted in the U.S. to compare longitudinal performance between lambs born in different seasons and reared in different production systems. Under the conditions of this study, organically managed lambs had similar performance to their conventionally managed counterparts. However, large differences in GIN infection risk over time existed between birth seasons and deworming regimens which have important implications for producer management decisions and genetic improvement programs.
Cracking etymological enigmas: unravelling the Greek and Latin languages’ contributions in marine taxa nomenclature
Authors: Kazanidis Georgios 
Published: December 2024

Abstract
Etymology is a key component in zoological nomenclature, often carrying key information for an organism. Greek and Latin languages have been used for centuries in synthesizing zoological names. However, there are surprisingly few studies about each language’s contribution, which is crucial for avoiding misunderstandings around names’ meaning and origin. This study examined the publications that described 1328 valid Echinodermata genera and presents results for 425 genera where etymology was provided by the authors. The analysis showed that 63.8% of genera names were composed exclusively of Greek elements, 28.0% were hybrid, 6.1% ‘other’, and 2.1% Latin. Language elements used in the formation of names were Greek (68.1%), ‘other’ (17.2%), or Latin (14.7%). The contribution of the Greek elements decreased after 1960, while the presence of hybrid increased. A decrease in the contribution of morphological elements was also observed after 1960, accompanied by an increased use of scientists’ surnames. Etymological analysis has unravelled and addressed long-lasting misconceptions about commonly used terminology. The number of studies where etymology is provided increased after 1960; this practice needs to be sustained as etymological transparency advances zoological knowledge and minimizes the risk for the development of misleading concepts in zoological nomenclature.
Recurrent evolution of breathing microtunnel system in terrestrial operculate snails (Gastropoda: Caenogastropoda: Cyclophoroidea)
Authors: Páll-Gergely Barna , Ruthensteiner Bernhard , Harl Josef , Magonyi Nóra M , Asami Takahiro , Krizsik Virág , Schwaha Thomas , Fehér Zoltán 
Published: December 2024

Abstract
The Cyclophoroidea are a group of land snails possessing an operculum that seals the aperture when the snail withdraws its body into the shell. Several groups have developed snorkel-like tubes, which are open at their outer ends, for gas exchange when the operculum closes the aperture. The tube of the cyclophorid subfamily Alycaeinae is, however, closed at its outer end. Recent investigations revealed that the tube of Alycaeinae, which starts to develop in subadults, is connected to several long microtunnels that run perpendicular to the tube and open near the umbilicus. We examined snails similar to Alycaeinae and discovered three types of gas-exchange systems that differ from the system in Alycaeinae. In Laotia, the microtunnels run to separate chambers, whereas in Platyrhaphe the microtunnels run to an internal tube. In both genera, the breathing device is already present in juveniles. Lastly, in Boucardicus there is no tube, but the microtunnels reach the common opening individually. Our molecular phylogeny, based on sequence data from the 28S, H3 and COI genes indicates that the microtunnel systems of these four groups have evolved independently. Based on these findings, three new subfamilies are proposed: Boucardicinae Páll-Gergely subfam. nov. (Hainesiidae), Laotiinae Páll-Gergely subfam. nov. (Diplommatinidae), and Platyrhaphidinae Páll-Gergely subfam. nov. (Cyclophoridae).
Patterns of Medication for Opioid Use Disorder During Pregnancy, 7 Clinical Sites, MATernaL and Infant clinical NetworK (MAT-LINK), 2014–2021
Authors: Tran Emmy L. , Dorsey Amanda N. , Miele Kathryn , Gilboa Suzanne M. , Gosdin Lucas , Terplan Mishka , Sanjuan Pilar M. , Seligman Neil S. , Wright Tanner , Wachman Elisha M. , Smid Marcela , Henninger Michelle , Leeman Lawrence , Schneider Patrick D. , Rood Kara , Louis Judette M. , Caveglia Sarah , Davidson Autumn , Shakib Julie , Shrestha Hira , Meaney-Delman Dana M. , Kim Shin Y. 
Published: December 2024

Objectives
To describe patterns of medication for opioid use disorder (MOUD) during pregnancies in the opioid use disorder (OUD) cohort of MAT-LINK, a sentinel surveillance network of pregnancies at US clinical sites.

Methods
Seven clinical sites providing care for pregnant people with OUD collected electronic health record data. Pregnancies were included in this analysis if (1) the pregnancy outcome occurred between January 2014 and August 2021, (2) the person had OUD, and (3) there was any electronic health record–documented MOUD during pregnancy. Analyses describing MOUD type, demographic characteristics, and timing during pregnancy were performed.

Results
Among 3911 pregnancies with any documented MOUD, more than 90% of pregnancies with methadone were to publicly insured people, which was greater than percentages for pregnancies with other MOUD. Buprenorphine with naloxone and naltrexone were two MOUD types that were increasingly common among pregnant people in recent years. In most pregnancies, prenatal care and MOUD were first documented in the same trimester. During the first, second, and third trimesters, there were 37%, 61%, and 91% of pregnancies with MOUD, respectively. Approximately 87% (n = 3412) had only 1 documented MOUD type, versus 2 or 3 types. However, discontinuity in MOUD across trimesters was still observed.

Conclusions
In MAT-LINK’s OUD cohort, the overall frequency of MOUD improved over the course of pregnancy. Contextual factors, such as insurance status and year of pregnancy outcome, might influence MOUD type. Prenatal care and MOUD might be facilitators for one another; however, there are still opportunities to improve early linkage and continuous access to both prenatal care and MOUD during pregnancy.
Validity of a Classification System for the Levels of Maternal Care
Authors: Vanderlaan Jennifer , Shen Jay , McDonough Ian K. 
Published: December 2024

OBJECTIVE:
To assess the content validity of the classification of maternal level of care of the American Hospital Association Database for research use.

METHODS:
This was a secondary data analysis where we classified the maternal level of care in the 2018 American Hospital Association Database and linked this to birth hospitalizations from five states in the 2016 and 2017 State Inpatient Databases: Delaware, Florida, Kentucky, Maryland, and Washington. We compared maternal level of care classification with birth volume quartiles, hospital size quartiles, and teaching status to predict the birth hospital for women with high OCI (Obstetric Comorbidity Index) scores and hospital-to-hospital transfers. We calculated the odds of birth at the highest-level hospital, controlling for maternal race, rural residence, primary payer, and state.

RESULTS:
People with high OCI scores and hospital-to-hospital transfer had increased odds of birth at hospitals classified as maternal level III or IV, large hospitals, and teaching hospitals. The probability of birth at the highest-level hospital for people with high OCI scores was increased 4.9% for a level III or IV hospital, 2.6% for a large hospital, and 1.2% for a teaching hospital. The probability of birth at the highest-level hospital for people with hospital transfer was increased 5.2% for a level III or IV hospital, 1.4% for a large hospital, and 14.4% for a teaching hospital.

CONCLUSION:
Researchers can classify the maternal level of care using the American Hospital Association Database to study maternal risk-appropriate care.