bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cómo generar conocimiento a partir de la innovación docente: Estudio de las villae romanas en Asturias
Authors: Piay Augusto Diego 
Published: October 2020

El proyecto que se describe nació con el firme propósito de introducir al alumnado de 2º curso del grado de Historia de la Facultad de Filosofía y Letras de la Universidad de Oviedo, que cursa la materia Historia Antigua de Roma, en los resortes de la investigación histórica, acercándolos al estudio de un fenómeno de gran interés para la historia antigua de Asturias como lo es la difusión de las villae (haciendas de carácter rural propiedad de individuos pudientes de la sociedad romana).
Durante el proyecto que se describirá, el alumnado, asistido en todo momento por el docente, aprendió el funcionamiento de los catálogos arqueológicos de Asturias, vaciando la información de interés para el cometido que nos habíamos marcado; elaboró fichas, empleando una base de datos creada ex profeso para este proyecto; revisó y trabajó con la bibliografía de referencia; y expuso los resultados de la investigación de forma coherente. Todo ello persiguiendo una triple motivación: la superación de la asignatura a partir del desarrollo de un proyecto motivador; la experiencia altamente formativa de trabajar con el método histórico; y el fin último del proyecto: la publicación de los resultados junto con el equipo docente, que supervisó toda la actividad.
Quantitative proteomics analysis of FFPE tumor samples reveals the influences of NET-1 siRNA nanoparticles and sonodynamic therapy on tetraspanin protein involved in HCC
Authors: Wu Bolin , Shang Haitao , Liang Xitian , Yang Huajing Yang Huajing , Jing Hui , Li Bo , Cheng Wen 
Published: October 2020

Abstract
Hepatocellular carcinoma (HCC) poses a severe threat to human health. The NET-1 protein has been proved to be strongly associated with HCC proliferation and metastasis in our previous study. Here, we established and validated NET-1 siRNA nanoparticles system to conduct targeted gene therapy of HCC xenograft in vivo with the aid of sonodynamic therapy (SDT). Then, a label-free proteome mass spectrometry workflow to analyze formalin-fixed and paraffin-embedded HCC xenograft samples collected in this study. The result showed that 78 proteins were differentially expressed after NET-1 protein inhibited. Among them, the expression of 61 proteins up-regulated and the expression of 17 proteins were significantly down-regulated. Of the differentially expressed proteins, the vast majority of Gene Ontology enrichment terms belong to the biological process. The KEGG pathway enrichment analysis showed that the 78 differentially expressed proteins significantly enriched in 45 pathways. We concluded that the function of the NET-1 gene is not only to regulate HCC but also to participate in a variety of biochemical metabolic pathways in the human body. Furthermore, the protein-protein interaction analysis indicated that the interactions of differentially expressed proteins are incredibly sophisticated. All the protein-protein interactions happened after the NET-1 gene has been silenced. Finally, our study also provides a useful proposal for targeted therapy based on tetraspanin proteins to treat HCC, and further mechanism investigations are needed to reveal a more detailed mechanism of action for NET-1 protein regulation of HCC.
Addressing the interaction between food insecurity, depression risk and informal work: findings of a cross-sectional survey among informal women workers with young children in South Africa.
Authors: Horwood Christiane , Lyn Haskins , Rachael Hinton , Catherine Connolly , Silondile Luthuli , Nigel Rollins 
Published: October 2020

Abstract
Background: There is a high burden of depression globally, including in South Africa. Maternal depression is associated with poverty, unstable income, food insecurity, and lack of partner support, and may lead to poor outcomes for mothers and children. In South Africa one-third of working women are in informal work, which is associated with socioeconomic vulnerability. Methods: A cross sectional survey explored work setting and conditions, food security and risk of depression among informal working women with young children (0-3years). Depression risk was assessed using the Edinburgh Postnatal Depression Score (EPDS) and Whooley score. Food insecurity was evaluated using USAID Household Food Insecurity Access Scale (HFIAS). Data was analysed using SPSS and Stata. Results: Interviews were conducted with 265 informal women workers. Types of work included domestic work, home-based work, informal employees and own account workers, most of whom were informal traders. Most participants (149/265; 56.2%) earned between US$70-200 per month, but some participants (79/265; 29.8%) earned less than US$70 per month, and few earned >US$200 per month (37/265; 14.0%). Many participants experienced mild (38/267; 14.3%), moderate (72/265; 27.2%) or severe (43/265; 16%) food insecurity. Severe food insecurity was significantly higher among participants with the lowest income compared to those with the highest income (p= 0.027). Women who received financial support from the baby’s father were less likely to be food insecure (p=0.03). Using EPDS scores, 22/265 (8.3%) women were designated as being at risk of depression. This was similar among postnatal women and women with older children. Household food insecurity was significantly associated with depression risk (p<0.001).Conclusions: Informal women workers were shown to be vulnerable with low incomes and high rates of food insecurity, thus increasing the risk for poor maternal health. However, levels of depression risk were low compared to previous estimates in South Africa, suggesting that informal workers may have high levels of resilience. Interventions to improve social protection, access to health services, and support for safe childcare in the workplace could improve the health and wellbeing of these mothers and support them to care for their children.
Inter-limb differences in patients following total hip arthroplasty four to five years after surgery: a cross-sectional study
Authors: John Stefanie , Weizel David , Heumann Anna Sophie , Fischer Anja , Orlowski Katja , Mrkor Kai-Uwe , Edelmann-Nusser Jürgen , Witte Kerstin 
Published: October 2020

Abstract
Backgroundː Total hip arthroplasty (THA) is a successful procedure to reduce pain and restore function in patients with end-stage hip osteoarthritis. However, whether or not pre-operatively existing deficits in strength, balance, range of motion (ROM) and gait are persisting several years post-surgery in the affected limb has not been thoroughly researched. Therefore, the aim of the study was to investigate inter-limb differences in patients who had undergone THA four to five years ago and to compare the values of the operated leg to controls. Methodsː Sixteen patients (age: 65.20±5.32 years) following unilateral THA (post-operation time: 4.7±0.7 years) and ten, healthy, age-matched controls (age: 60.85±7.57 years) were examined for maximum isometric hip muscle strength, active ROM of the hip joint, balance and gait on both limbs. Paired t-tests were used to assess the inter-limb differences in the THA group. Analyses of covariance (ANCOVA) were performed to compare values of the operated leg to controls, using age as a covariate. Results: The analysis of inter-limb differences in patients following THA revealed significant deficits on the operated side for hip abduction strength (p=0.02), for hip flexion ROM (p<0.01) and for balance in terms of the length of center of pressure (COP) (p=0.04). Compared to values of the controls, the patients demonstrated significantly reduced hip strength in flexion, extension and abduction (p<0.05) on the operated leg as well as reduced ROM measures in hip flexion, extension and abduction (p<0.05). Conclusions: Inter-limb differences as well as reduced hip strength and hip ROM were still present four to five years after THA. These persisting asymmetries and deficits in patients following THA may be one explanation for the decrease in health-related quality of life (HRQoL) seen in patients over the years after surgery. Postoperative training focusing on strengthening hip muscles should be continued months and years after hip replacement in order to compensate persisting deficits and asymmetries. Trial registration: DRKS, DRKS00016945. Registered 12 March 2019 – Retrospectively registered, http://www.drks.de/DRKS00016945
Frailty and healthcare utilisation across care settings among community-dwelling older adults in Singapore
Authors: LIXIA GE , Yap Chun Wei , Heng Bee Hoon , Tan Woan Shin 
Published: October 2020

Abstract
Background Frailty is frequently found to be associated with increased healthcare utilisation in western countries, but little is known in Asian population. This study was conducted to investigate the association between frailty and healthcare utilisation in different care settings among community-dwelling older adults in Singapore.Methods Data from a population health survey among community-dwelling adults were linked with an administrative database to retrieve data of healthcare utilisation (including government primary care clinic visits, specialised outpatient clinic visits, emergency department visits, day surgery and hospitalisations) occurred during a six-month look-back period and six-month post-baseline respectively. Baseline frailty status was measured using the five-item FRAIL scale, which was categorised into three groups: robust (0), pre-frail (1–2), and frail (3–5). Negative binomial regression was applied to examine the association between frailty with respective healthcare utilisation (dependent variables), controlling for other confounding variables. Results In our sample of 701 older adults, 64.8% were of robust health, 27.7% were pre-frail, and 7.6% were frail. Compared to the robust group, frail individuals had a higher rate of specialised outpatient clinic visits (incidence rate ratio (IRR): 2.8, 95% confidence interval (CI): 1.2-6.5), emergency department visits (IRR: 3.1, 95%CI: 1.1-8.1), day surgery attendances (IRR: 6.4, 95%CI: 1.3-30.9), and hospitalisations (IRR: 6.7, 95%CI: 2.1-21.1) in the six-month period prior to the baseline and in subsequent six months (IRR: 3.3, 95%CI: 1.6-7.1; 6.4, 2.4-17.2; 5.8, 1.3-25.8; 13.1, 4.9-35.0; respectively), controlling for covariates. Conclusions Frailty was positively associated with the number of specialised outpatient clinic visits, emergency department visits, day surgeries and hospitalisations occurred during 6 months prior to and after the baseline. As frailty is a potentially reversible health state with early screening and intervention, providing preventive activities that delay the onset or progression of frailty should have potential effect on delaying secondary and tertiary care utilisation.
The Cerebral Mechanism of Acupuncture for Chronic Insomnia with Gastrointestinal Disorder: Protocol for a Randomized Controlled Trial
Authors: Peng Wei , Hong Xiaojuan , Huangfu Yaru , Sun Zhao , Shen Wei , Feng Fen , Gong Liang , Shen Zhifu , Guo Baojun , Zhang Leixiao , Wang Yanan , Zhao Ying , Zhu Tianmin , Hu Youping , Yu Siyi 
Published: October 2020

Abstract
BackgroundMany patients with chronic insomnia disorder (CID) have gastrointestinal (GI) symptoms. First-line insomnia medications do not treat GI problems. Acupuncture has a comprehensive regulative action on both CID and GI disorder and is receiving increasing attention. Recent studies indicate that both CID and GI diseases may cause abnormal brain activity. However, the neurological mechanism underlying the effect of acupuncture on such diseases is still unclear. The aim of this study is to explore the pathological mechanisms of CID with GI discomfort, as well as the main response characteristics of acupuncture treatment from multiple perspectives using multimodal magnetic resonance imaging (MRI).MethodsA total of 60 participants with CID and GI disorders will be randomly divided into two groups (real acupuncture group and sham acupuncture group; ratio of 1:1). Patients will receive 20 sessions (five sessions per week) of real acupuncture treatment or sham acupuncture treatment. The primary outcome is the aggregate score on the Pittsburgh Sleep Quality Index. Secondary outcomes are scores on the Gastrointestinal Symptom Rating Scale, Self-Rating Anxiety Scale, and Self-Rating Depression Scale. Multimodal MRI scans and clinical assessments will be performed both at baseline and post-treatment. Another 30 age-, sex-, and education-matched healthy subjects will be recruited as controls and will receive MRI scans and clinical evaluations. DiscussionThis study aims to provide scientiﬁc evidence for the mechanism of acupuncture in treating CID with GI disorder using multimodal MRI imaging data on brain structure, function, and metabolism. Trial registrationThe study was registered in the Chinese Clinical Trial Registry on July 11, 2018. (ChiCTR1800017092)
Genome-Wide Characterization of B-BOX Gene Family and Their Responses to Light Quality and Cold Stress in Tomato
Authors: Bu Xin , Wang Xiujie , Yan Jiarong , Zhang Ying , Zhou Shunyuan , Sun Xin , Yang Youxin , Ahammed Golam Jalal , Liu Yufeng , Xu Tao , Qi Hongyan , Qi Mingfang , Wang Feng , Li Tianlai 
Published: October 2020

Abstract
Background: Perceiving incoming environmental information is critical for optimizing plant growth and development. Multiple B-box proteins (BBXs) play essential roles in light-dependent developmental processes in plants. However, whether BBXs function as a signal integrator between light and temperature in tomato plants remains elusive.Results: In this study, 31 SlBBX genes were identified from the newly released tomato (Solanum lycopersicum) genome sequences, and were clustered into five subgroups with phylogenetic analysis. Gene structure and protein motif analyses showed relatively high conservation of closely clustered SlBBX genes within each subgroup; however, genome mapping analysis indicated the uneven distribution of the SlBBX genes on tomato chromosomes. Synteny analysis indicated that segmental duplication events happened in the expansion of the SlBBX genes in tomato. Promoter cis-regulatory elements prediction indicated that SlBBX genes were highly responsive to light, hormones, and stress conditions. Furthermore, the transcript analysis revealed that various SlBBX genes differed significantly in expression after exposure to different light quality and low temperatures, while 61.3% of SlBBX genes were responsive to both light and low temperatures. Conclusions: Our study presented a genome-wide survey of SlBBX gene family in tomato, and emphasized their functions in perceiving light quality and low temperature, which may improve the current understanding of SlBBX gene functions in integrating light and temperature signals for plant adaptation to adverse environments.
Association of Intrinsic Factors with Non-contact Low Back Pain Among Fast Bowlers Aged between 15 – 19 Years in Division 1 Boys’ Schools in Colombo
Authors: Jayasinghe Udeesh Satheesha , Swaris Imasha , Weerasooriya Diluka , Mayura Dushan , Bandara Sachinda , Disanayake Kavindi , Perera Angage Dilani Priyashanthi 
Published: October 2020

Abstract
Objective: Cricket fast bowlers are identified as the greatest injury risk players who are more prone to sustain with low back pain (LBP). The current study was aimed to investigate how intrinsic factors associate with LBP among fast bowlers aged between 15-19 years. A descriptive cross-sectional study was conducted with one hundred and two (102) fast bowlers in Colombo division 1 boys’ schools in Sri Lanka. Bowlers were prospectively monitored over the competition period of 2019 cricket season and the demographic data, severity of LBP, general characteristics and intrinsic factors related data were recorded.Results: Thirty-seven (43.5%) of the subjects were presented with LBP. Reduction of quadriceps strength of dominant side, hamstring strength of non-dominant side and increased ankle dorsiflexion of non-dominant side were found to be significantly associated with non-contact LBP (P<0.05). There were significant differences for the quadriceps strength of dominant side and hamstring strength and ankle dorsiflexion of non-dominant side between the fast bowlers with and without LBP. The results indicated that reduced quadriceps muscle strength of dominant side, reduced hamstring muscle strength and high ankle dorsiflexion of non-dominant side have an important role in predisposing a fast bowler to have an increase in non-contact LBP.
Molecular clamp stabilised Spike protein for protection against SARS-CoV-2
Authors: Watterson Daniel , Wijesundara Danushka , Modhiran Naphak , Mordant Francesca , Li Zheyi , Avumegah Michael , McMillan Christopher , Lackenby Julia , Guilfoyle Kate , Amerongen Geert van , Stittelaar Koert , Cheung Stacey , Bibby Summa , Daleris Mallory , Hoger Kym , Gillard Marianne , Radunz Eve , Jones Martina , Hughes Karen , Hughes Ben , Goh Justin , Edwards David , Scoble Judith , Pearce Lesley , Kowalczyk Lukasz , Phan Tram , La Mylinh , Lu Louis , Pham Tam , Zhou Qi , Brockman David , Morgan Sherry , Lau Cora , Tran Mai , Tapley Peter , Letelier Fernando Villalon , Barnes James , Young Andrew , Jaberolansar Noushin , Scott Connor , Isaacs Ariel , Amarilla Alberto , Khromykh Alexander , Reading Patrick , Ranasinghe Charani , Subbarao Kanta , Munro Trent , Young Paul , Chappell Keith 
Published: October 2020

Abstract
Efforts to develop and deploy effective vaccines against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) continue at pace with more than 30 candidate vaccines now in clinical evaluation. Here we describe the preclinical development of an adjuvanted, prefusion-stabilised Spike (S) protein “Sclamp” subunit vaccine, from rational antigen design through to assessing manufacturability and vaccine efficacy. In mice, the vaccine candidate elicits high levels of neutralising antibodies to epitopes both within and outside the receptor binding domain (RBD) of S, as well as broadly reactive and polyfunctional S-specific CD4+ and cytotoxic CD8+ T cells. We also show protection in Syrian hamsters, which has emerged as a robust animal model for pulmonary SARS-CoV-2 infection. No evidence of vaccine enhanced disease was observed in animal challenge studies and pre-clinical safety was further demonstrated in a GLP toxicology study in rats. The Sclamp vaccine candidate is currently progressing rapidly through clinical evaluation in parallel with large-scale manufacture for pivotal efficacy trials and potential widespread distribution.
Coronary Artery Bypass Graft Surgery in Tanzania: 5 Years of Experience at Jakaya Kikwete Cardiac Institute
Authors: Janabi Mohamed Yakubu , Nyawawa Evarist , Nyangasa Bashir , Ramadhan William Ramadhani , Hamis Ramadhani Hassan , Hussein Hassanali , Sharau Godwin , Naizibijwa Majani , Sulende Khuboja , Mlawi Vivienne Aiyana , Shemu Tulizo , Bhalia Smita , Kisenge Peter , Kimambo Delilah , Kifai Engerasia , Tatizo Waane , Mayala Henry , Khanbhai Khuzema , Waandwi Boniface , Muhozya Angela 
Published: October 2020

Abstract
Cardiac surgery is not widely available in most developing countries, and most patients have no choice but to live in morbid conditions and managed conservatively or the few who are referred abroad for surgical procedures costs the respective countries millions of hard earned foreign currency. The World Health Organization projects that over the next ten years the continent of Africa will experience the largest increase in death rates from cardiovascular disease. The Jakaya Kikwete Cardiac Institute (JKCI) is a government owned National Specialized and Teaching Hospital that serves patients from all the regions of the United Republic of Tanzania with a population of nearly 60,000,000 people and also serves beyond the borders (Rwanda, Burundi, DR Congo, South Sudan, Comoro, Malawi and Zambia) for advanced cardiovascular medical, intervention, vascular and open heart surgery, the Institute was established in 2015. Methods: Here we report all patients who underwent coronary artery bypass surgery grafting only performed at the Centre since its inauguration in 2015- till 2019. Data were collected for basic demography, diagnosis, investigations, clinical and surgical outcome parameters. Results: A total of 85 patients with heart diseases and underwent coronary artery bypass surgery grafting (CABG) are analysed in this study. There were 64 (75%) male and 21 (25%) female patients. Their age ranged from 41–85 years old with almost half 42 (49%) of the cohort being between the age between 61–70 years old. Most of the patients had two or more grafts and an internal mammary artery graft was used over 80% of the procedures. The overall 30-day mortality was 7.1%, incidence of stroke 0.2%, duration of mechanical ventilation was an average of 9.98 hours and intensive care unit (ICU) stay post CABG was an average of 6.48 days and final discharge from the centre ranged from 10–16 days.Conclusion: This study has demonstrated that coronary artery bypass surgery grafting in low/middle income country is safe and feasible. A sustainable program demands highest level of governmental support as seen in this case, and a dedicated multidisciplinary team with profound know how in cardiac pathologies. Furthermore, a need for good local data to know the prevalence of coronary disease is mandatory to determine the magnitude of coronary artery disease in each country.
Down-Regulation of LAMP3 Inhibits the Proliferation and Migration of HSCC
Authors: Qiu Xiaoxia , Li Juanjuan , Yang Keke , Jiang Song , Yin Yong , Bian Longjun , Huang Xiaorong , Sun Yi , Zhang Zhenxin 
Published: October 2020

Abstract
Background: Lysosomal-associated membrane glycoprotein 3 (LAMP3) has been shown to be highly expressed in various types of tumors. It is associated with their poor prognosis, proliferation, invasion, and metastasis, respectively. However, the role of LAMP3 in hypopharyngeal squamous cell carcinoma (HSCC) is unclear. Objective:This article aims to investigate the role of LAMP3 in the proliferation and metastasis of HSCC. Method: Detection of the expression of LAMP3 in clinical HSCC and paired adjacent healthy tissue samples by using immunohistochemistry. Furthermore, LAMP3 was knocked out of the HSCC cell line, FaDu, using a lentivirus vector and the in vitro biological effects of LAMP3 knockdown in FaDu cells were studied. Results: Immunohistochemistry results showed that LAMP3 was highly expressed in HSCC. Additionally, it was verified at the mRNA and protein levels that lentiviral transduction effectively down-regulated the expression of LAMP3 in FaDu cells. Cell colony formation assays and CCK8 proliferation assays showed that down-regulation of LAMP3 on FaDu cells inhibited cell proliferation and growth rate. Wound healing experiments and transwell experiments showed that significant down-regulation of LAMP3 on FaDu cells inhibited cell migration. Conclusion:Collectively, our results suggest that LAMP3 is a useful therapeutic target for the treatment of hypopharyngeal cancer.