bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pull-out and Critical Embedment Length of Grouted Rebar Rock Bolts-Mechanisms When Approaching and Reaching the Ultimate Load
Authors: Høien Are Håvard , Li Charlie C. , Zhang Ning 
Published: January 2021

AbstractRock bolts are one of the main measures used to reinforce unstable blocks in a rock mass. The embedment length of fully grouted bolts in the stable and competent rock stratum behind the unstable rock blocks is an important parameter in determining overall bolt length. It is required that the bolt section in the stable stratum must be longer than the critical embedment length to ensure the bolt will not slip when loaded. Several series of pull tests were carried out on fully grouted rebar bolts to evaluate the pull-out mechanics of the bolts. Bolt specimens with different embedment lengths and water/cement ratios were installed in either a concrete block of one cubic meter or in steel cylinders. Load displacement was recorded during testing. For some of the bolts loaded beyond the yield load, permanent plastic steel deformation was also recorded. Based on the test results, three types of failure mechanisms were identified, corresponding to three loading conditions: (1) pull-out below the yield strength of the bolt steel; (2) pull-out between the yield and ultimate loads, that is, during strain hardening of the steel; and (3) steel failure at the ultimate load. For failure mechanisms 2 and 3, it was found that the critical embedment length of the bolt included three components: an elastic deformation length, a plastic deformation length and a completely debonded length due to the formation of a failure cone at the borehole collar.
Complications of external cerebrospinal fluid drainage in aneurysmal subarachnoid haemorrhage
Authors: Arts Sebastian , van Lindert Erik J. , Aquarius Rene , Bartels Ronald H. M. A. , Boogaarts Hieronymus D. 
Published: January 2021

Abstract
Background
The need for external cerebrospinal fluid (CSF) drains in aneurysmal subarachnoid haemorrhage (aSAH) patients is common and might lead to additional complications.

Objective
A relation between the presence of an external CSF drain and complication risk is investigated.

Methods
A prospective complication registry was analysed retrospectively. We included all adult aSAH patients admitted to our academic hospital between January 2016 and January 2018, treated with an external CSF drain. Demographic data, type of external drain used, the severity of the aSAH and complications, up to 30 days after drain placement, were registered. Complications were divided into (1) complications with a direct relation to the external CSF drain and (2) complications that could not be directly related to the use of an external CSF drain referred to as medical complications

Results
One hundred and forty drains were implanted in 100 aSAH patients. In total, 112 complications occurred in 59 patients. Thirty-six complications were drain related and 76 were medical complications. The most common complication was infection (n = 34). Drain dislodgement occurred 16 times, followed by meningitis (n = 11) and occlusion (n = 9). A Poisson model showed that the mean number of complications raised by 2.9% for each additional day of drainage (95% CI: 0.6–5.3% p = 0.01).

Conclusion
Complications are common in patients with aneurysmal subarachnoid haemorrhage of which 32% are drain-related. A correlation is present between drainage period and the number of complications. Therefore, reducing drainage period could be a target for further improvement of care.
Simple fistula-in-ano: is it all simple? A systematic review
Authors: Litta F. , Parello A. , Ferri L. , Torrecilla N. O. , Marra A. A. , Orefice R. , De Simone V. , Campennì P. , Goglia M. , Ratto C. 
Published: January 2021

Abstract
Background
Simple anal fistula is one of the most common causes of proctological surgery and fistulotomy is considered the gold standard. This procedure, however, may cause complications. The aim of this systematic review was to assess the surgical treatment of simple anal fistula with traditional and sphincter-sparing techniques.

Methods
A literature research was performed using PubMed, Cochrane, and Google Scholar to identify studies on the surgical treatment of simple anal fistulas. Observational studies and randomized clinical trials were included. We assessed the risk of bias of included studies using the Jadad scale for randomized controlled trials, and the MINORS Scale for the remaining studies.

Results
The search returned 456 records, and 66 studies were found to be eligible. The quality of the studies was generally low. A total of 4883 patients with a simple anal fistula underwent a sphincter-cutting procedure, mainly fistulotomy, with a weighted average healing rate of 93.7%, while any postoperative continence impairment was reported in 12.7% of patients. Sphincter-sparing techniques were adopted to treat 602 patients affected by simple anal fistula, reaching a weighted average success rate of 77.7%, with no study reporting a significant postoperative incontinence rate. The postoperative onset of fecal incontinence and the recurrence of the disease reduced patients’ quality of life and satisfaction.

Conclusions
Surgical treatment of simple anal fistulas with sphincter-cutting procedures provides excellent cure rates, even if postoperative fecal incontinence is not a negligible risk. A sphincter-sparing procedure could be useful in selected patients.
Taxonomic study of a new green alga, Annulotesta cochlephila gen. et sp. nov. (Kornmanniaceae, Ulvales, Ulvophyceae), growing on the shells of door snails
Authors: Namba Noriaki , Nakayama Takeshi 
Published: January 2021

AbstractSome algae are known to grow on shellfish shells. Most of these have been reported in aquatic environments. The species specificity for substrate shells varies, and some algae grow only on the shells of a certain species of shellfish, such asPseudocladophora conchopheria(Cladophorales, Ulvophyceae) onLunella coreensis(Trochida, Gastropoda). There are very few reports of algae that grow on land snails. In this study, we discovered green algae growing on the shells of six species of door snails (Clausiliidae) from nine localities in Japan. These green algae formed a green mat composed of thalli embedded in the extracellular matrix. The thallus was composed of aggregated oval cells and peripheral branched filaments. The cells possessed a single parietal chloroplast with a pyrenoid surrounded by two starch sheaths and transversed by a thylakoid. Oil droplets in the cell and ring-like structures on the cell wall surface were frequently observed. The 18S rDNA sequences of all shell-attached algae on different clausiliid species from different localities were almost identical and formed a new clade in the family Kornmanniaceae (Ulvales, Ulvophyceae). No other algae forming visible colonies on the clausiliid shell were found. These findings indicate the presence of specificity between the alga and clausiliid shells. Based on the results of morphological observation and molecular phylogenetic analysis, we propose a new genus and new species of shell-attached green alga,Annulotesta cochlephila.
Estimation of Parental Effects Using Polygenic Scores
Authors: Balbona Jared V. , Kim Yongkang , Keller Matthew C. 
Published: January 2021

AbstractOffspring resemble their parents for both genetic and environmental reasons. Understanding the relative magnitude of these alternatives has long been a core interest in behavioral genetics research, but traditional designs, which compare phenotypic covariances to make inferences about unmeasured genetic and environmental factors, have struggled to disentangle them. Recently, Kong et al. (2018) showed that by correlating offspring phenotypic values with the measured polygenic score of parents’ nontransmitted alleles, one can estimate the effect of “genetic nurture”—a type of passive gene–environment covariation that arises when heritable parental traits directly influence offspring traits. Here, we instantiate this basic idea in a set of causal models that provide novel insights into the estimation of parental influences on offspring. Most importantly, we show how jointly modeling the parental polygenic scores and the offspring phenotypes can provide an unbiased estimate of the variation attributable to the environmental influence of parents on offspring, even when the polygenic score accounts for a small fraction of trait heritability. This model can be further extended to (a) account for the influence of different types of assortative mating, (b) estimate the total variation due to additive genetic effects and their covariance with the familial environment (i.e., the full genetic nurture effect), and (c) model situations where a parental trait influences a different offspring trait. By utilizing structural equation modeling techniques developed for extended twin family designs, our approach provides a general framework for modeling polygenic scores in family studies and allows for various model extensions that can be used to answer old questions about familial influences in new ways.
Uremic toxin indoxyl sulfate promotes proinflammatory macrophage activation by regulation of β-catenin and YAP pathways
Authors: Li Ying , Yan Jing , Wang Minjia , Lv Jing , Yan Fei , Chen Jin 
Published: January 2021

AbstractEvidence has been shown that indoxyl sulfate (IS) could impair kidney and cardiac functions. Moreover, macrophage polarization played important roles in chronic kidney disease and cardiovascular disease. IS acts as a nephron-vascular toxin, whereas its effect on macrophage polarization during inflammation is still not fully elucidated. In this study, we aimed to investigate the effect of IS on macrophage polarization during lipopolysaccharide (LPS) challenge. THP-1 monocytes were incubated with phorbol 12-myristate-13-acetate (PMA) to differentiate into macrophages, and then incubated with LPS and IS for 24 h. ELISA was used to detect the levels of TNFα, IL-6, IL-1β in THP-1-derived macrophages. Western blot assay was used to detect the levels of arginase1 and iNOS in THP-1-derived macrophages. Percentages of HLA-DR-positive cells (M1 macrophages) and CD206-positive cells (M2 macrophages) were detected by flow cytometry. IS markedly increased the production of the pro-inflammatory factors TNFα, IL-6, IL-1β in LPS-stimulated THP-1-derived macrophages. In addition, IS induced M1 macrophage polarization in response to LPS, as evidenced by the increased expression of iNOS and the increased proportion of HLA-DR+ macrophages. Moreover, IS downregulated the level of β-catenin, and upregulated the level of YAP in LPS-stimulated macrophages. Activating β-catenin signaling or inhibiting YAP signaling suppressed the IS-induced inflammatory response in LPS-stimulated macrophages by inhibiting M1 polarization. IS induced M1 macrophage polarization in LPS-stimulated macrophages via inhibiting β-catenin and activating YAP signaling. In addition, this study provided evidences that activation of β-catenin or inhibition of YAP could alleviate IS-induced inflammatory response in LPS-stimulated macrophages. This finding may contribute to the understanding of immune dysfunction observed in chronic kidney disease and cardiovascular disease.
The Roles of Familiarity and Context in Processing Chinese Xiehouyu: An ERP Study
Authors: Wang Xiaolu , Wang Yizhen , Tian Wanning , Zheng Wei , Chen Xiaoli 
Published: January 2021

AbstractThis study conducts an ERP experiment to explore the online processing mechanism of Chinese xiehouyu, a subcategory of Chinese idiomatic expressions with a metaphorical two-part allegorical saying, regarded as a non-literal language construct. Using a 2 × 2 design, (high familiarity (HF)/low familiarity (LF)) × (literally-biasing context (LC)/metaphorically-biasing context (MC)), the researchers have obtained the following findings: (1) familiarity plays an important role in Chinese xiehouyu processing, i.e. the metaphorical meaning of a HF Chinese xiehouyu can be directly activated while that of a LF one has to be derived from its literal meaning first; (2) contextual information also weighs in the process, i.e. the metaphorical meaning of a Chinese xiehouyu can be promoted in MC condition but suppressed in LC condition; (3) the interactive effect of familiarity and contextual information can be explained by the career of metaphor hypothesis; and (4) the Standard Pragmatic Model (SPM) of non-literal languages can explain the processing of LF xiehouyu, and the Direct Access Model (DAM) may to some extent account for the mechanism of HF one but fails to explain the case of LF one, while the Graded Salience Hypothesis (GSH) can provide an acceptable explanation for the processing mechanism of Chinese xiehouyus of varied familiarity.
Fracture formation due to differential compaction under glacial load: a poro-elastoplastic simulation of the Hugin Fracture
Authors: Landschulze Karin , Landschulze Marcus 
Published: January 2021

AbstractThe Hugin Fracture, discovered in 2011, is an approximately 3.5 km long seafloor fracture in the North Sea. This fracture was unexpected and, due to the geology in the North Sea no obvious explanation could be found. In our study, we adopt the hypothesis that the Hugin fracture was formed by differential compaction controlled by glacial load. We construct a simplified 2D geomechanical model partly covered by top load (ice sheet) and test this hypothesis. We employ transient poro-elastoplastic simulation with a finite element method. For the simulations, we had to make assumptions regarding the material properties, because the fracture is located in-between well locations. We used descriptions from drilling site survey reports and literature values and performed seismic matching form well paths to the Hugin Fracture. Nearby well data were only partly useful due to incomplete logging in the first 400 m below seafloor. To overcome this problem, we introduced a mixing k-value which allows us to easily change the material properties from pure clay to sand. Changing the mixing k-value for each simulation provided information about the limits and robustness of the simulation results. Simulation results show isotropic stress and strain distribution in the horizontally layered, isotropic part of the model that is totally covered by the ice. In the central, channelized part of the model a composite stress and strain pattern develops with sub-vertical focus areas tangential to channel edges. Low stress, strain and deformation values under total load increase drastically soon after the load starts to decrease, resulting in the development of fractures along the focussed zones. Surface deformation such as formation of compaction ridges above stiff clay-filled channels and depression associated with plastic deformation is observed. A fracture and associated surface deformation develop above the shallowest sand-filled channel, very much resembling the observed geometry at the Hugin Fracture. The simulation supports the formation hypothesis for the Hugin Fracture as a compaction fracture and suggests that thin ice sheets may induce differential compaction to a depth of several hundred meters.
Biochemical markers for clinical monitoring of tissue perfusion
Authors: Janotka Marek , Ostadal Petr 
Published: January 2021

AbstractThe assessment and monitoring of the tissue perfusion is extremely important in critical conditions involving circulatory shock. There is a wide range of established methods for the assessment of cardiac output as a surrogate of oxygen delivery to the peripheral tissues. However, the evaluation of whether particular oxygen delivery is sufficient to ensure cellular metabolic demands is more challenging. In recent years, specific biochemical parameters have been described to indicate the status between tissue oxygen demands and supply. In this review, the authors summarize the application of some of these biochemical markers, including mixed venous oxygen saturation (SvO2), lactate, central venous–arterial carbon dioxide difference (PCO2 gap), and PCO2 gap/central arterial-to-venous oxygen difference (Ca–vO2) for hemodynamic assessment of tissue perfusion. The thorough monitoring of the adequacy of tissue perfusion and oxygen supply in critical conditions is essential for the selection of the most appropriate therapeutic strategy and it is associated with improved clinical outcomes.
Mesenchymal glioblastoma-induced mature de-novo vessel formation of vascular endothelial cells in a microfluidic device
Authors: Amemiya Takeo , Hata Nobuhiro , Mizoguchi Masahiro , Yokokawa Ryuji , Kawamura Yoichiro , Hatae Ryusuke , Sangatsuda Yuhei , Kuga Daisuke , Fujioka Yutaka , Takigawa Kosuke , Akagi Yojiro , Yoshimoto Koji , Iihara Koji , Miura Takashi 
Published: January 2021

AbstractHigh vascularization is a biological characteristic of glioblastoma (GBM); however, an in-vitro experimental model to verify the mechanism and physiological role of vasculogenesis in GBM is not well-established. Recently, we established a self-organizing vasculogenic model using human umbilical vein endothelial cells (HUVECs) co-cultivated with human lung fibroblasts (hLFs). Here, we exploited this system to establish a realistic model of vasculogenesis in GBM. We developed two polydimethylsiloxane (PDMS) devices, a doughnut-hole dish and a 5-lane microfluidic device to observe the contact-independent effects of glioblastoma cells on HUVECs. We tested five patient-derived and five widely used GBM cell lines. Confocal fluorescence microscopy was used to observe the morphological changes in Red Fluorescent Protein (RFP)-HUVECs and fluorescein isothiocyanate (FITC)-dextran perfusion. The genetic and expression properties of GBM cell lines were analyzed. The doughnut-hole dish assay revealed KNS1451 as the only cells to induce HUVEC transformation to vessel-like structures, similar to hLFs. The 5-lane device assay demonstrated that KNS1451 promoted the formation of a vascular network that was fully perfused, revealing the functioning luminal construction. Microarray analysis revealed that KNS1451 is a mesenchymal subtype of GBM. Using a patient-derived mesenchymal GBM cell line, mature de-novo vessel formation could be induced in HUVECs by contact-independent co-culture with GBM in a microfluidic device. These results support the development of a novel in vitro research model and provide novel insights in the neovasculogenic mechanism of GBM and may potentially facilitate the future detection of unknown molecular targets.
Role of MDA5 in regulating CXCL10 expression induced by TLR3 signaling in human rheumatoid fibroblast-like synoviocytes
Authors: Saruga Tatsuro , Imaizumi Tadaatsu , Kawaguchi Shogo , Seya Kazuhiko , Matsumiya Tomoh , Sasaki Eiji , Sasaki Norihiro , Uesato Ryoko , Ishibashi Yasuyuki 
Published: January 2021

AbstractC-X-C motif chemokine 10 (CXCL10) is an inflammatory chemokine and a key molecule in the pathogenesis of rheumatoid arthritis (RA). Melanoma differentiation-associated gene 5 (MDA5) is an RNA helicase that plays a role in innate immune and inflammatory reactions. The details of the regulatory mechanisms of CXCL10 production and the precise role of MDA5 in RA synovitis have not been fully elucidated. The aim of this study was to examine the role of MDA5 in regulating CXCL10 expression in cultured human rheumatoid fibroblast-like synoviocytes (RFLS). RFLS was stimulated with Toll-like receptor 3 (TLR3) ligand polyinosinic:polycytidylic acid (poly I:C), a synthetic double-stranded RNA mimetic. Expression of interferon beta (IFN-β), MDA5, and CXCL10 was measured by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), western blotting, and enzyme-linked immunosorbent assay. A neutralizing antibody of IFN-β and siRNA-mediated MDA5 knockdown were used to determine the role of these molecules in regulating CXCL10 expression downstream of TLR3 signaling in RFLS. Poly I:C induced IFN-β, MDA5, and CXCL10 expression in a concentration- and time-dependent manner. IFN-β neutralizing antibody suppressed the expression of MDA5 and CXCL10, and knockdown of MDA5 decreased a part of CXCL10 expression (p < 0.001). The TLR3/IFN-β/CXCL10 axis may play a crucial role in the inflammatory responses in RA synovium, and MDA5 may be partially involved in this axis.
Anthracycline-induced cytotoxicity in the GL261 glioma model system
Authors: Tavener Amber M. , Phelps Megan C. , Daniels Richard L. 
Published: January 2021

AbstractGlioblastoma (GBM) is a lethal astrocyte-derived tumor that is currently treated with a multi-modal approach of surgical resection, radiotherapy, and temozolomide-based chemotherapy. Alternatives to current therapies are urgently needed as its prognosis remains poor. Anthracyclines are a class of compounds that show great potential as GBM chemotherapeutic agents and are widely used to treat solid tumors outside the central nervous system. Here we investigate the cytotoxic effects of doxorubicin and other anthracyclines on GL261 glioma tumor cells in anticipation of novel anthracycline-based CNS therapies. Three methods were used to quantify dose-dependent effects of anthracyclines on adherent GL261 tumor cells, a murine cell-based model of GBM. MTT assays quantified anthracycline effects on cell viability, comet assays examined doxorubicin genotoxicity, and flow cytometry with Annexin V/PI staining characterized doxorubicin-induced apoptosis and necrosis. Dose-dependent reductions in GL261 cell viability were found in cells treated with doxorubicin (EC50 = 4.9 μM), epirubicin (EC50 = 5.9 μM), and idarubicin (EC50 = 4.4 μM). Comet assays showed DNA damage following doxorubicin treatments, peaking at concentrations of 1.0 μM and declining after 25 μM. Lastly, flow cytometric analysis of doxorubicin-treated cells showed dose-dependent induction of apoptosis (EC50 = 5.2 μM). Together, these results characterized the cytotoxic effects of anthracyclines on GL261 glioma cells. We found dose-dependent apoptotic induction; however at high concentrations we find that cell death is likely necrotic. Our results support the continued exploration of anthracyclines as compounds with significant potential for improved GBM treatments.