bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiplex Lateral Flow Immunochromatographic Assay Is an Effective Method to Detect Carbapenemases Without Risk of OXA-48-like Cross Reactivity
Authors: Kon Hadas , Abramov Shirin , Frenk Sammy , Schwartz David , Shalom Ohad , Adler Amos , Carmeli Yehuda , Lellouche Jonathan 
Published: January 2021

Abstract
Background: It is essential to detect carriers of carbapenemase-producing Enterobacterales in order to implement infection control measures. The objectives of this study was to evaluate the NG-Test® CARBA 5 (CARBA 5) assay for detection of five carbapenemases and to assess the cross reactivity of other OXA-type carbapenemases with the OXA-48-like specific antibodies. Methods: A total of 197 Enterobacterales isolates were tested. To evaluate the cross reactivity, 73 carbapenem-resistant A. baumannii, harboring OXA-type variants, were tested. Polymerase chain reaction (PCR) served as gold standard for carbapenemase identification. Results: Excellent agreement was found between PCR and CARBA 5, for all but one isolate. The single false positive result (a blaSME positive S. marcescens isolate) was incorrectly positive for blaOXA-48 by CARBA 5. No cross reactivity was observed. The sensitivity and specificity were 100.0% and 98.0%, respectively. Conclusions: The CARBA 5 assay is highly sensitive and specific and is recommended as a tool for the detection of five carbapenemases.
Surgical Treatment of Primary Intracranial and Extracranial Communicating Leiomyosarcoma with One Case Recurred
Authors: wang zeyi , pu kuairong , zhang wencai , chen peng , shao dongchuan , zhao nan 
Published: January 2021

Abstract
backgroundTo analyze and summarize the clinical features and diagnosis and treatment experience on primary intracranial and extracranial communicating leiomyosarcoma to deepen clinicians’understanding of the rare disease.Cases presentationThe clinical data and recurrence of a patient who was diagnosed with primary intracranial and extracranial communicating leiomyosarcoma admitted to the Neurosurgery Department, The Affiliated Ganmei Hospital of Kunming Medical University in May 2015 were retrospectively analyzed. According to the patient data, two successful surgical operations were performed and the surgeries went well, with more than 2-year and 8-month follow-up so far. No obvious complications were found after hospital discharge, and follow-up was continued.ConclusionsSurgical removal is the most important and effective treatment means for primary intracranial and extracranial communicating leiomyosarcoma currently.
System Coupling and Coordinating Evaluation of Green Low-carbon Development in Coal Enterprises
Authors: Xu Yinna , Zhao Guohao 
Published: January 2021

Abstract
Green low-carbon development is the direction for coal enterprises to cope with overcapacity, industrial restructuring and enterprise transformation. Based on the connotation of green low-carbon development, this paper deconstructed the green low-carbon development of coal enterprises into a process of coupling and coordinated development of green low-carbon system (GLCS) and innovation development system (IDS) from a systematic perspective, further deconstructed the green low-carbon system into two subsystems of environment (ES) and resources(RS), and deconstructed the innovation development system into two subsystems of innovation (IS) and growth(GS), built the coupling and coordination evaluation model and grey relational degree model of green low-carbon development of coal enterprises, and made an empirical analysis of China Shenhua Energy Company Limited ("CSEC") and China National Coal Group Corp (“CNCGC”) in 2010-2017. The empirical analysis showed that the coupling and coordinating level of GLCS and IDS of CSEC and CNCGC is both not high, but there are differences in evolutionary trends and causes, and the coupling and interaction mechanism needs to be strengthened; the coupling coordination degree between ES and IS is good, and between RS and GS is the worst in CSEC, and the coupling coordination degree between RS and GS is highly correlated with the coupling coordination degree between GLS and IDS, and improving the level of IS is an important direction for CSEC; But in CNCGC the coupling coordination degree between subsystems fluctuates greatly, and there is no persistent good and persistent bad coupling, but in the past two years, the level of coupling coordination among subsystems has a rising trend, and the level of RS and the degree of coupling coordination between ES and IS have the highest correlation with the degree of coupling coordination between GLS and IDS. Coal enterprises should improve the interaction mechanism among subsystems, strengthen the driving role of green innovation, and promote green low-carbon development.
E2F2 Inhibition Induces Autophagy to Impair Metastasis via the PI3K/Akt/mTOR Pathway in Gastric Cancer
Authors: Li Hui , Zhao Shufen , Shen Liwei , Wang Peige , Liu Shihai , Ma Yingji , Liang Zhiwei , Wang Gongjun , Lv Jing , Qiu Wensheng 
Published: January 2021

Abstract
BackgroundE2F2 is a member of the E2F transcription factor family and has important but not fully understood biological functions in cancers. The pro- and antitumor functions of E2F2 in some cancer types are controversial. However, the biological role of E2F2 in gastric cancer (GC) remains unclear.MethodsE2F2 expression from multiple gene expression databases was analyzed. Kaplan-Meier plots and Cox regression were used to analyze the prognostic value of E2F2 expression. The correlation between E2F2 and tumor immune infiltration was investigated using the Tumor Immune Estimation Resource (TIMER) database. The functions and pathways of E2F2 and its 50 most highly correlated genes in terms of expression pattern were analyzed using Database Annotation, Visualization, and Integrated Discovery (DAVID) software. We used immunohistochemistry, real-time quantitative polymerase chain reaction, and western blotting to detect the expression level of E2F2 in GC. We further investigated the effects of E2F2 on phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling, autophagy, and the migration and invasion of GC cells by the wound healing assay, Transwell assay, western blotting, and transmission electron microscopy.ResultsE2F2 was highly expressed in both GC tissues and cells compared with normal gastric tissues/cells in public datasets and our validation experiments. High E2F2 expression was associated with poor overall survival (OS). In addition, the expression of E2F2 in GC had a strong correlation with a variety of immune markers. E2F2 overexpression promoted the migration and invasion of GC cells in vitro through the inhibition of PI3K/Akt/mTOR-mediated autophagy. In contrast, inhibition of E2F2 inhibited the migration and invasion of GC cells in vitro by activating PI3K/Akt/mTOR-mediated autophagy.ConclusionThis study provides multilevel evidence for the significance of E2F2 in the pathogenesis of GC and its potential as a biomarker for GC. E2F2 was highly expressed in GC, and high E2F2 expression was associated with the characteristics of invasive tumors and poor prognosis. E2F2 had potential modulatory effects on tumor immunity. We found a novel function of E2F2 in the regulation of PI3K/Akt/mTOR-mediated autophagy and the downstream process of cell migration and invasion. Our results may provide novel targets and strategies for the diagnosis and treatment of GC.
Spatial epidemiology of COVID-19 infection through the first outbreak (March-May 2020) in the city of Mashhad, Iran
Authors: Mansouritorghabeh Hasan , Bagherimoghaddam Ahmad , MohammadEbrahimi Shahab , Eslami Saeid , Raouf-Rahmati Amene , Hamer Davidson H , Kiani Behzad 
Published: January 2021

Abstract
Background: Since December 2019, SARS-CoV-2 infection has converted to a severe threat to global health. It is now considered as the fifth worldwide pandemic problem. This study aims to explore spatial-time distribution of COVID-19 in the first outbreak of COVID-19 in the second major city of Iran (Mashhad). The results will pave the way for better tracking of COVID-19.Methods: Data were collected from two tertiary hospitals in Mashhad in June 2020. They included demographic findings and residential address of the patients with confirmed COVID-19 disease by polymerase chain reaction test. The univariate logistic regression model was used to assess the influence of age and sex on mortality. For spatial-time analysis, after calculating empirical Bayesian rate for every neighborhood, the local Moran's I statistic was used to quantify spatial autocorrelation of COVID-19 frequency at the city neighborhood level.Results: Of 1,535 confirmed cases of COVID-19 included in this study, 951 (62%) were male. Odds of death for patients over 60 years of age was more than three times higher (odds ratio [OR]: 3.7, CI [2.8-4.8]) than for those under the 60 years. In addition, the ratio of relative mortality for male patients was significantly higher than the female (OR: 1.58, CI [1.2-2]). The univariate regression model also revealed that odds of death increased along with increase in duration of hospitalization secondary to COVID-19 disease (OR: 1.02, IQR [1.01-1.02]). The downtown area had a significant high-high cluster throughout much of the study period (March-May 2020). Conclusions: Collection of geographic information system (GIS) map data on SARS-CoV-2 provides insight into clusters of infection and high risk places for COVID-19 transmission. GIS-
Psycho-social wellbeing among in-school adolescents of Sri Lanka; prevalence of selected attributes and associated factors
Authors: Wellappuli Nalinda , Gunawardena Nalika Sepali , Mahesh PK Buddhika , Gunawardena Shanthi , Wickramasinghe Champika , Lokubalasooriya Ayesha , Peiris Renuka , Liyanage Chinthika , Hathurusinghe Lalindi , Mahagamage Kanchana , Senanayake Sameera 
Published: January 2021

Abstract
Background

An optimum adolescence is associated with a psycho-social and cognitive development. These developments affects the interactions in the day-to-day activities of young people and have impacts on their lives beyond the adolescence age-group. The prevalence of psycho-social problems among adolescents are on the rise. The aim of this study was to estimate the prevalence and factors associated with the selected attributes of psycho-social well-being among 13 to 17-year in-school adolescents of Sri Lanka using the Global School Health Survey (GSHS).

Methods

GSHS, was a cross-sectional study conducted among 3,650 adolescents of aged 13-17, attending government schools. Data were collected using a self-administered questionnaire. Four selected psychosocial well-being outcomes (i.e. being bullied, loneliness, feeling anxious, suicidal ideation and practices) were assessed with questions for 11 potential associated factors of those. The sample was selected through a two-staged cluster sampling technique. Multivariable analysis was done with multiple logistic regression to determine the independent associated factors.

Results

Majority of the respondents belonged to the 13-15 age group (66.5%) and with 51.1% were females. More than one third of the participants (37.7%, 95%CI 36.1-39.5) had experienced bullying 30 days prior to the study. ‘Loneliness’ and ‘feeling anxious’ had been experienced by 9.0% (95%CI 8.0-10.0) and 4.7% (95%CI 3.9- 5.4), respectively. The prevalence of planning an attempt of suicide was 6.3%, while 6.6% had at least attempted suicide once. Multivariable analysis revealed that ‘lacked parental support’ and ‘being engaged in a fight’ were independently associated with being bullied, feeling lonely and considering attempting suicide. Mutual associations of the four outcomes with each other indicate that being bullied was significantly associated with loneliness and feeling anxious. In addition, loneliness was independently associated ‘considering an attempt of suicide’ (OR=2.9, 95%CI=2.0 to 4.3). Feeling anxious was also associated with considering attempting suicide (OR=3.4, 95%CI= 2.1 to 5.4).

Conclusion

This study demonstrated that the prevalence of psycho-social wellbeing is among school going adolescents is a major public health problem in Sri Lanka. School-based interventions to address these issues should be designed with the goal of increasing adolescents’ awareness of the measures of raising the psycho-social wellbeing.
Online single-shot pulse reconstruction for optimizing a seeded X-ray free-electron laser
Authors: Zeng Li , Gu Duan , Feng Chao , Liu Bo , Zhao Zhentang 
Published: January 2021

Abstract
X-ray free-electron lasers (FELs) hold promising prospects for opening up opportunities for ultra-fast sciences at the atomic and molecular system. A precise knowledge of temporal information of FEL pulses is the central issue for experiments. Here we proposed and demonstrated an online diagnostic method to determine the FEL temporal profiles at the Shanghai Soft X-ray FEL facility. This robust method, designed for seeded FELs, allows researchers to acquire real-time longitudinal profiles of FEL pulses with a resolution better than 3 fs. Based on this method, for the first time, we can directly measure various properties of FEL pulses from two stages and correlations between them online. This helps us to further understand the physics and realize the lasing of a stable, nearly fully coherent soft X-ray FEL through a two-stage harmonic up-shift configuration. This method also provides an intuitive way for precise detection and control of the relative timing between electron beams and external optical lasers.
Fotografi sebagai Media Kreativitas Anggota Dekranasda Jawa Barat untuk Memasarkan Produk Unggulannya
Authors: Haqqu Rizca , Nugrahani Rah Utami , Nurfebiaraning Sylvie , Yusanto Freddy 
Published: January 2021

Pengabdian banyarakat dengan mitra sasar pelaku UKM yang tergabung dalam anggota Dekranasda Jawa Barat bertujuan untuk memberikan transfer knowledge. Adapun pelatihan yang diberikan adalah memberikan wawasan tentang fotografi dan pemasaran melalui media sosial. Pelatihan ini dilakukan dengan konsep workshop fotografi dengan materi seperti komposisi, shot size, pengenalan metode pemotretan objek, prinsip dan teknik untuk melakukan setting eksposure pada kamera yang akan dipakai, teknik pencahayaan (lighting), pemilihan properti yang tepat untuk meningkatkan nilai sebuah produk hingga trik memotret produk dengan kualitas profesional namun dengan perangkat seadanya seperti smartphone ataupun camera pocket. Dengan pelatihan ini, diharapkan peserta mampu meningkatkan kualitas foto produk mereka sehingga mampu menarik lebih banyak calon pembeli yang berimbas pada pengingkatan penghasilan para anggota UKM Dekranasda Jawa Barat dalam menjalankan bisnis onlinenya. antusiasme para pelaku UKM untuk mendapatkan ilmu tentang fotografi dan pemasaran online sangat tinggi. Rata-rata materi yang disampaikan mudah dipahami dan dapat dipalikasikan secara langsung. Dengan kata lain pelatihan yang diberikan efektif meningkatkan kualitas foto produk sehingga akan berdampak pada naiknya jumlah minat pembeli.
VELHICE E CONTEMPORANEIDADE: REFLEXÕES PSICOSSOCIAIS SOBRE O APRENDIZADO DE INFORMÁTICA EM UM GRUPO DE IDOSOS
Authors: Pontin de Medeiros Eduardo , Hübner Wolff Suzana 
Published: January 2021

Este artigo se propõe a refletir sobre o significado do aprendizado da informática para um grupo de idosos participantes de um programa social de uma universidade, situada na região metropolitana de Porto Alegre, Rio Grande do Sul. Por meio de entrevistas, análise de conteúdo e com o auxilio de um referencial teórico de abordagem biopsicossocial, observamos que o curso representa principalmente a necessidade de exercitar a autonomia pessoal, com o uso do computador. Refletimos ainda que o fato de o idoso estar em grupo, nesse processo de aprendizagem, contribui, segundo eles, para a compreensão e aceitação do processo de envelhecimento humano.palavras-chave Envelhecimento Humano. Informática. Autonomia. Aprendizado. Grupo. abstract This article intends to reflect on the learning of informatics among a group of elderly participants in a university located in the metropolitan region of Porto Alegre, Rio Grande do Sul. Through content analysis of interviews and with the help of a biopsychosocial reference, we note that the main reason for these seniors pursuing this course was due to the need for autonomy in the computer and how being in a group contributes to the understanding and acceptance the procedures of human aging. keywords Aging. Informatics. Autonomy. Learning. Group.
MOTIVAÇÕES E EXPECTATIVAS DAS IDOSAS COM O CURSO “AGENTES SOCIAIS DE LAZER”
Authors: Martins Gomes Clarissa , Kunzler Mateus , Dos Santos Cardoso Stephanie , Kruger Gonçalves Andréa , Pettenuzzo Santiago Diná , Jost Blessmann Eliane 
Published: January 2021

Com o objetivo de incentivar a participação das pessoas idosas como sujeitos das ações desenvolvidas no projeto foi desenvolvido o curso “agentes sociais de lazer”. Para investigar sobre a motivação para participar do curso e a expectativa em ser agente social foram realizadas entrevistas semi-estruturadas com 8 alunas do curso. Da análise do conteúdo das entrevistas emergiram 3 categorias: a aprendizagem como motivação, o protagonismo das alunas nas ações do projeto e o significado do ser agente social. Os resultados obtidos sugerem que as pessoas idosas querem continuar aprendendo, querem participar, querem aplicar o conhecimento, querem ser reconhecidas como úteis e capazes. A vontade de aprender serviu como motivação para participarem do curso e a atuação como agente social está proporcionando a participação ativa na sociedade. palavras-chaveAgente Social. Lazer. Idosas. Motivações. Expectativas. abstract In order to encourage the participation of older people as subjects of the actions developed in the project we developed the course “social agents of leisure”. In order to investigate the motivation to attend the course and expectations on being a social agent, semi-structured interviews were conducted involving eight students of the course. From the analysis of the interviews emerged three categories: learning as motivation, the role of students in the project’s actions and the meaning of being a social agent. The results suggest that older people want to keep learning, want to participate, they want to apply their knowledge, and they want to be recognized as useful and capable. The willingness to learn has served as motivation to attend the course and acting as the agent is providing social participation in society. Keywords Social Agent. Leisure. Elderly. Motivation. Expectations.
VALORACIÓN GERONTOLÓGICA MULTIDIMENSIONAL EN COMUNIDAD
Authors: Tuzzo Rosario , Araújo Nybia , Sosa Ana Paula , Achard Paula , García Leticia 
Published: January 2021

La multidimensionalidad del proceso de envejecimiento, se encuentra determinada por factores biológicos, psicológicos, sociales, culturales, ambientales y económicos. Para el estudio de este proceso complejo, se requiere de la integración de equipos interdisciplinarios que aborden sus múltiples aristas. Las diferentes profesiones sanitarias que integran el equipo gerontológico que trabaja en comunidad, deben realizar una adecuada selección y adaptación de los instrumentos de evaluación, para su empleo en este ámbito, incluyendo aspectos como: sencillez, rapidez, bajo costo, portabilidad y posibilidad de aplicación por distintas profesiones de la salud, así como de aprendizaje por parte de estudiantes. Estas técnicas no sustituyen los estudios psicométricos específicos, pero son de utilidad para detectar la presencia de factores de riesgo que ameriten una evaluación más profunda y también para uniformizar y comparar los resultados de los diferentes equipos participantes. Para la valoración multidimensional del adulto mayor en el ámbito comunitario, se ha diseñado un instrumento portátil. “La Ficha del Residente”, integrada por los siguientes ítems: Datos sociodemográficos, antecedentes de salud, valoración social y ambiental, valoración funcional, valoración cognitiva y valoración afectiva El objetivo de este trabajo ha sido evaluar a una muestra de 54 residentes de viviendas públicas de la ciudad de Montevideo, de edades entre los 65 y 94 años y realizar un diagnóstico preliminar, como forma de despistar a tiempo y de forma objetiva alteraciones en las distintas esferas. Se exponen los ítems y técnicas que integran la Ficha, los resultados obtenidos de su aplicación y las estrategias de abordaje a implementarse.
ROBÓTICA NA ASSISTÊNCIA AO IDOSO COM DOENÇA DE ALZHEIMER: AS VANTAGENS E OS DESAFIOS DESSA INTERVENÇÃO.
Authors: Do Carmo Elisangela Gisele , Zazzetta Marisa Silvana , Costa José Luiz Riani 
Published: January 2021

A crescente demanda de idosos com doença de Alzheimer (DA) faz da dependência da assistência uma realidade cada vez maior, neste sentido, a robótica surge como uma das possíveis alternativas de amparo a estes idosos. O objetivo deste trabalho foi identificar mediante revisão de literatura a robótica de assistência para os idosos com DA, no mundo e no Brasil e, discutir o impacto social e as limitações causados pela utilização dessas tecnologias. Utilizou-se o método de revisão sistemática com pesquisa em bases de dados científicos, como SciELO, PubMED, LILACS, IEEE Xplore, Web of Science, SAGE, Scopus, entre outros. A ferramenta StArt (State of the Art through Systematic Review) foi utilizada com a finalidade de sumarizar os estudos. Selecionamos artigos de acesso livre e acesso restrito. Os descritores controlados e não controlados foram pesquisados em portais de terminologias em saúde: Ciências da Saúde (DeCS) e Medical Subject Headings (MeSH). Identificou-se 132 artigos científicos, destes, 19 foram selecionados pela ferramenta StArt e considerados elegíveis para esta revisão. Dentre os projetos desta revisão, observa-se que a grande maioria podem ser utilizada em idosos nos estágios leves e moderada da DA, dado que, nestes estágios, funções motoras e cognitivas ainda podem se encontrar preservadas. Conforme visto nos resultados desta revisão, diversas tecnologias apresentadas necessitam da assistência do cuidador, para programações prévias dos equipamentos. Assim, preparar os cuidadores torna-se importante para o correto funcionamento destas tecnologias. A presença do cuidador é fator chave e não pode ser substituída. Embora a aquisição de tecnologias avançadas, como por exemplo, um robô facilite a autonomia e possibilite maior independência, o fortalecimento de vínculos afetivos e das relações interpessoais são aspectos insubstituíveis para a qualidade de vida do idoso. A pesquisa evidencia de forma clara, como vários fatores socioeconômicos e culturais de um país, influenciam no processo de implementação da robótica de assistência para idosos acometidos por DA.