bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary fibre and protein do not synergistically influence insulin, metabolic or inflammatory biomarkers in young obese Göttingen minipigs
Authors: Xu Yetong , Curtasu Mihai Victor , Bach Knudsen Knud Erik , Hedemann Mette Skou , Theil Peter Kappel , Lærke Helle Nygaard 
Published: August 2020

AbstractThe effects of dietary fibre (DF) and protein on insulin response, lipidaemia and inflammatory biomarkers were studied in a model experiment with juvenile obese Göttingen minipigs. After 20 weeks feeding on a high-fat fructose-rich low-DF diet, forty-three 30-week-old minipigs (31·3 (sem 4·0) kg body weight) were allocated to low- or high-DF and -protein diets for 8 weeks in a 2 × 2 factorial design. High DF contents decreased (P = 0·006) while high protein increased (P < 0·001) the daily gain. High protein contents increased fasting plasma concentrations of glucose (P = 0·008), NEFA (P = 0·015), ghrelin (P = 0·008) and non-fasting LDL:HDL ratios (P = 0·015). High DF increased ghrelin (P = 0·036) and C-peptide levels (P = 0·011) in the non-fasting state. High protein increased the gene expression of fructose-bisphosphatase 1 in liver tissue (P = 0·043), whereas DF decreased fatty acid synthase expression in adipose tissue (P = 0·035). Interactions between DF and protein level were observed in the expression of leptin receptor in adipose tissue (P = 0·031) and of PPARγ in muscle (P = 0·018) and adipose tissue (P = 0·004). In conclusion, high DF intake reduced weight gain and had potential benefit on β-cell secretory function, but without effect on the lipid profile in this young obese model. High dietary protein by supplementing with whey protein did not improve insulin sensitivity or lipidaemia, and combining high DF with high protein did not alleviate the risk of metabolic abnormalities.
Short- and long-term effects of rearing dairy calves with contact to their mother on their reactions towards humans
Authors: Waiblinger Susanne , Wagner Kathrin , Hillmann Edna , Barth Kerstin 
Published: August 2020

AbstractIn this Research Communication we address the hypotheses that reduced contact with humans during the first week of life would impair the relationship of dairy calves reared in dam-calf-contact systems to humans in comparison with artificially reared animals, but that this difference would vanish over time. Artificially reared calves (Artificial)that had been separated from their mother within 12 h after birth were bottle-fed with colostrum for 5 d and thereafter sucked milk from an automatic milk feeder. Animals reared with dam-calf contact (Dam-contact) were kept in the calving pen with their dam for 5 d, and then had permanent access to the cow barn and thus to their dam. Calves were weaned at an age of 12 weeks and kept in young stock groups mixed of both treatments until integration into the cow herd. We tested the animals’ relationship with humans by assessing the animals' responses towards an unfamiliar person in an avoidance distance (AD) test in the home environment at 4 weeks of age, at 15 months and at 33 months. In calves, we additionally measured AD in a novel arena after a stationary person test.Artificialanimals had lower AD, i.e. showed lower level of fear, thanDam-contactcalves. However, the AD inDam-contactcalves decreased with increasing number of days they experienced assistance for suckling. Further, there was no significant difference in later ages. In conclusion, gentle human contact in combination with feeding during the first 5 d of life improved calves' relationship to humans leading to differences between the two treatments as well as within theDam-contactcalves. Potential effects under different conditions regarding quantity and quality of human-animal interactions need further research.
Investigating data-driven biological subtypes of psychiatric disorders using specification-curve analysis
Authors: Beijers Lian , van Loo Hanna M. , Romeijn Jan-Willem , Lamers Femke , Schoevers Robert A. , Wardenaar Klaas J. 
Published: August 2020

AbstractBackgroundCluster analyses have become popular tools for data-driven classification in biological psychiatric research. However, these analyses are known to be sensitive to the chosen methods and/or modelling options, which may hamper generalizability and replicability of findings. To gain more insight into this problem, we used Specification-Curve Analysis (SCA) to investigate the influence of methodological variation on biomarker-based cluster-analysis results.MethodsProteomics data (31 biomarkers) were used from patients (n = 688) and healthy controls (n = 426) in the Netherlands Study of Depression and Anxiety. In SCAs, consistency of results was evaluated across 1200 k-means and hierarchical clustering analyses, each with a unique combination of the clustering algorithm, fit-index, and distance metric. Next, SCAs were run in simulated datasets with varying cluster numbers and noise/outlier levels to evaluate the effect of data properties on SCA outcomes.ResultsThe real data SCA showed no robust patterns of biological clustering in either the MDD or a combined MDD/healthy dataset. The simulation results showed that the correct number of clusters could be identified quite consistently across the 1200 model specifications, but that correct cluster identification became harder when the number of clusters and noise levels increased.ConclusionSCA can provide useful insights into the presence of clusters in biomarker data. However, SCA is likely to show inconsistent results in real-world biomarker datasets that are complex and contain considerable levels of noise. Here, the number and nature of the observed clusters may depend strongly on the chosen model-specification, precluding conclusions about the existence of biological clusters among psychiatric patients.
Higher fire frequency impaired woody species regeneration in a south-eastern Amazonian forest
Authors: Cury Roberta Thays dos Santos , Balch Jennifer Kakareka , Brando Paulo Monteiro , Andrade Rafael Barreto , Scervino Renata Picolo , Torezan José Marcelo Domingues 
Published: August 2020

AbstractUnderstorey wildfires harm tropical forests by affecting natural regeneration, but the trajectories of fire-disturbed forests after disturbance are poorly understood. To fill this gap, we conducted experimental burns in a transitional forest between the Amazon forests and the Brazilian Savanna (Cerrado) and investigated their effects on plant community diversity of regeneration. The experiment consisted of three 50-ha plots that between 2004 and 2010 were burned either annually (six times), every three years (thrice) or not at all (Control). To evaluate early post-fire recovery, we recorded grass occurrence and regenerating stems (≤1 cm in diameter at breast height). We noted that high fire-frequency plots had a reduction of species richness (62%) and abundance (84%) and were associated with floristic and structural changes, dominance of few species and increase of grass colonization when compared with low fire-frequency. We observed that resprouts were the main pathway for forest restoration in both burned regimes, particularly in low fire-frequency. However, the forest can recover from fires by means of resprouting, until a threshold in fire frequency is reached, when resprouts and seedlings declined for most of the species, with a few fire-tolerant species becoming dominant.
COVID-19 in India: transmission dynamics, epidemiological characteristics, testing, recovery and effect of weather
Authors: Chanda Arnab 
Published: August 2020

Abstract
The spread of COVID-19 is recent in India, which has within 4 months caused over 190 000 infections, as of 1 June 2020, despite four stringent lockdowns. With the current rate of the disease transmission in India, which is home to over 1.35 billion people, the infection spread is predicted to be worse than the USA in the upcoming months. To date, there is a major lack of understanding of the transmission dynamics and epidemiological characteristics of the disease in India, inhibiting effective measures to control the pandemic. We collected all the available data of the individual patients, cases and a range of parameters such as population distribution, testing and healthcare facilities, and weather, across all Indian states till May 2020. Numerical analysis was conducted to determine the effect of each parameter on the COVID-19 situation in India. A significant amount of local transmission in India initiated with travellers returning from abroad. Maharashtra, Tamil Nadu and Delhi are currently the top three infected states in India with doubling time of 14.5 days. The average recovery rate across Indian states is 42%, with a mortality rate below 3%. The rest 55% are currently active cases. In total, 88% of the patients experienced symptoms of high fever, 68% suffered from dry cough and 7.1% patients were asymptomatic. In total, 66.8% patients were males, 73% were in the age group of 20–59 years and over 83% recovered in 11–25 days. Approximately 3.4 million people were tested between 1 April and 25 May 2020, out of which 4% were detected COVID-19-positive. Given the current doubling time of infections, several states may face a major shortage of public beds and healthcare facilities soon. Weather has minimal effect on the infection spread in most Indian states. The study results will help policymakers to predict the trends of the disease spread in the upcoming months and devise better control measures.
Interventional recanalisation of long segment aortic atresia
Authors: Huntgeburth Michael , Ahmad Wael , Brunkwall Jan , Sreeram Narayanswami 
Published: August 2020

AbstractWe report the case of a long-segment aortic atresia as the cause for therapy resistant arterial hypertension in a young adult. Recanalization was achieved interventionally by wire-crossing and stent implantation with subsequent normalization of blood pressure.
Dietary total antioxidant capacity (TAC), general and central obesity	indices and serum lipids among adults: An updated systematic review and	meta-analysis
Authors: Farhangi Mahdieh Abbasalizad , Vajdi Mahdi , Fathollahi Pourya 
Published: August 2020

Abstract. Background: In the present meta-analysis, we aimed to summarize the relationship between dietary total antioxidant capacity (TAC), general and central obesity indices and lipid profile in adult population. Methods: The electronic databases of Web of Sciences, PubMed, Scopus and Cochrane library were searched for relevant studies from inception to October 2019. The effect size was indicated as weighted mean difference (WMD) and 95% confidence intervals (CI) by using random effects model. The I2 index and Cochran’s Q-test were used for evaluating heterogeneity. Results: From 2,469 studies identified, thirty-four studies (nineteen cross-sectional studies, thirteen cohort studies, two case-control studies) were included in the meta-analysis. According to our results, higher categories of TAC were associated with significantly lower serum triglyceride concentartions (TG; WMD: −7.58; CI: −11.42, −3.75; P < 0.001) and waist circumference (WC; WMD: −1.17; 95% CI: −1.47, −0.87; P < 0.001); while no significant change in body mass index (BMI; WMD: −0.17; 95% CI: −0.35, 0.01; P = 0.12), high density lipoprotein cholesterol (HDL-C; WMD: 0.61; 95% CI: −0.16, 1.40; P = 0.12), low density lipoprotein cholesterol (LDL-C; WMD: 1.34; 95% CI: −0.61, 3.30; P = 0.17) and total cholesterol (TC; WMD: 1.19; 95% CI: −1.46, 3.855; P = 0.37) was reported. Conclusion: Higher dietary TAC was related to reduced prevalence of central obesity, reduced WC and TG concentrations in the current meta-analysis. Moreover, subgroup analysis showed that TAC measurement index, geographical area, dietary assessment tool, health status and gender were potential sources of heterogeneity.
Schnittstellenkoordination von systemübergreifenden Hilfen für Kinder und Jugendliche mit psychischen Beeinträchtigungen
Authors: Böge Isabel , DeKeijzer Deborah , Fegert Jörg M. , Schulze Ulrike 
Published: August 2020

Zusammenfassung. Schnittstellen zwischen psychosozialen Hilfesystemen stellen Sollbruchstellen einer gelingenden systemübergreifenden Behandlung psychisch erkrankter Kinder/Jugendlicher dar. Daten zu Schnittstellenmanagement in Deutschland liegen bisher nicht vor. Ziel der Studie ASpeKT war es, zu vier Zeitpunkten (T1 Aufnahme, T2 Entlassung, T3 6 Mo und T4 12 Mo nach Entlassung) den Ist-Stand von installierten Hilfen bei psychisch erkrankten Kindern und Jugendlichen ( n=191) zu erheben, Patientenzufriedenheit zu evaluieren sowie dies an zwei, strukturell unterschiedlichen Standorten (Klinik A/Klinik B) zu vergleichen. Zu T2 erhielten 13 % der Patienten keine Hilfen. 43,6 % (A) bzw. 70,4 % (B) erhielten Psychotherapie (PT), 67,3 % (A) bzw. 80,3 % (B) Jugendhilfemaßnahmen (JH), 37,6 % (A) bzw. 16.9 % (B) schulische Unterstützung. Gute Schnittstellenkoordination ist prognostisch essentiell, um den meist noch komplexen Hilfebedarf psychisch erkrankter Kinder/Jugendlicher nach stationärer Behandlung abzubilden.
The Tidal Response in the Mesosphere/Lower Thermosphere to the Madden‐Julian Oscillation Observed by SABER
Authors: Kumari Komal , Oberheide Jens , Lu Xian 
Published: August 2020

AbstractA statistical study of 18 years of diurnal temperature tides observed by the SABER instrument on board the TIMED satellite reveals a substantial response of the tides in the upper atmosphere (>60 km) to the Madden‐Julian Oscillation (MJO) in the tropical troposphere. Nonmigrating tidal amplitudes are modulated at the intraseasonal MJO periods up to ~25% relative to the seasonal mean, twice as much as for the migrating tides (~10%). We fully characterize the tidal response for active MJO days as a function of season and MJO location as prescribed by the MJO index. The MJO modulation of the tides was predicted by models but could not be unequivocally observed before. Our results further point to an important role of background winds that partly cause a different response for equatorial and nonequatorial tidal modes in different seasons, which has implications for the MJO imprint on the ionospheric dynamo region.
Vegetation Response to Elevated CO2 Slows Down the Eastward Movement of the 100th Meridian
Authors: Zhang Cicheng , Yang Yuting , Yang Dawen , Wang Zhengrong , Wu Xiuchen , Zhang Shulei , Zhang Wenjie 
Published: August 2020

AbstractThe 100th meridian is a conceptual arid‐humid divide that effectively bisects the continent of the United States into the humid east and arid west. Here, we examine the historical and future shifts of the 100th meridian from atmospheric, hydrologic, and agroecological perspectives. We find that the effective arid‐humid divide regarding vegetation greenness and runoff has been moving toward west with a mean shift of 0.73° (1982–2015) and 2.08° (1961–2010), respectively, suggesting decreased agroecological and hydrologic aridity in the region. For future periods, the agroecological divide shows a persistent westward movement, whereas eastward shifts of the 100th meridian are detected from the atmospheric and hydrologic perspectives. Nevertheless, the eastward movements of the atmospheric and hydrologic divide substantially slow down when the vegetation response to elevated atmospheric CO2 is accounted for. This finding demonstrates that CO2 fertilization on vegetation could largely alleviate the warming‐induced drying tendency around the 100th meridian region.
Storm‐Time Modeling of the African Regional Ionospheric Total Electron Content Using Artificial Neural Networks
Authors: Okoh Daniel , Habarulema John Bosco , Rabiu Babatunde , Seemala Gopi , Wisdom Joshua Benjamin , Olwendo Joseph , Obrou Olivier , Matamba Tshimangadzo Merline 
Published: August 2020

AbstractThis paper presents the development of a storm‐time total electron content (TEC) model over the African sector for the first time. The storm criterion used was |Dst| ≥ 50 nT and Kp ≥ 4. We have utilized Global Positioning System (GPS) observations from 2000 to 2018 from about 252 receivers over the African continent and surroundings within spatial coverage of 40°S–40°N latitude and 25°W–60°E longitude. To increase data coverage in areas devoid of ground‐based instrumentation including oceans, we used the available radio occultation Constellation Observing System for Meteorology, Ionosphere, and Climate (COSMIC) TEC from 2008 to 2018. The model is based on artificial neural networks which are used to learn the relationship between TEC and the corresponding physical/geophysical input parameters representing factors which influence ionospheric variability. An important result from this effort was the inclusion of the time history of the geomagnetic activity indicators
which improved TEC modeling by about 5% and 12% in middle and low latitudes, respectively. Overall, the model performs comparatively well with, and sometimes better than, the earlier single station modeling efforts even during quiet conditions. Given that this is a storm‐time model, this result is encouraging since it is challenging to model ionospheric parameters during geomagnetically disturbed conditions. Statistically, the average root‐mean‐square error (RMSE) between modeled and GPS TEC is 5.5 TECU (percentage error = 30.3%) and 5.0 TECU (percentage error = 30.4%) for the Southern and Northern Hemisphere midlatitudes respectively compared to 7.5 TECU (percentage error = 22.0%) in low latitudes.
The oxytocin receptor signalling system and breast cancer: a critical review
Authors: Liu Huiping , Gruber Christian W. , Alewood Paul F. , Möller Andreas , Muttenthaler Markus 
Published: August 2020

AbstractBreast cancer is making up one-quarter of all new female cancer cases diagnosed worldwide. Breast cancer surgeries, radiation therapies, cytotoxic chemotherapies and targeted therapies have made significant progress and play a dominant role in breast cancer patient management. However, many challenges remain, including resistance to systemic therapies, tumour recurrence and metastasis. The cyclic neuropeptide oxytocin (OT) elicits a plethora of biological responses via the oxytocin receptor (OTR) in both the central and peripheral nervous system, including social bonding, stress, maternal behaviour, sexual activity, uterus contraction, milk ejection and cancer. As a typical member of the G protein-coupled receptor family, OTR represents also an intriguing target for cancer therapy. There is emerging evidence that OTR plays a role in breast cancer development and progression, and several breast cancer cell lines express OTR. However, despite supporting evidence that OT lowers breast cancer risks, its mechanistic role in breast cancer development and the related signalling pathways are not fully understood. Here, we review the current knowledge of the OT/OTR signalling system in healthy breast tissue as well as in breast cancer, and discuss OTR as a potential therapeutic target for breast cancer management.
CD146, from a melanoma cell adhesion molecule to a signaling receptor
Authors: Wang Zhaoqing , Xu Qingji , Zhang Nengwei , Du Xuemei , Xu Guangzhong , Yan Xiyun 
Published: August 2020

AbstractCD146 was originally identified as a melanoma cell adhesion molecule (MCAM) and highly expressed in many tumors and endothelial cells. However, the evidence that CD146 acts as an adhesion molecule to mediate a homophilic adhesion through the direct interactions between CD146 and itself is still lacking. Recent evidence revealed that CD146 is not merely an adhesion molecule, but also a cellular surface receptor of miscellaneous ligands, including some growth factors and extracellular matrixes. Through the bidirectional interactions with its ligands, CD146 is actively involved in numerous physiological and pathological processes of cells. Overexpression of CD146 can be observed in most of malignancies and is implicated in nearly every step of the development and progression of cancers, especially vascular and lymphatic metastasis. Thus, immunotherapy against CD146 would provide a promising strategy to inhibit metastasis, which accounts for the majority of cancer-associated deaths. Therefore, to deepen the understanding of CD146, we review the reports describing the newly identified ligands of CD146 and discuss the implications of these findings in establishing novel strategies for cancer therapy.
Cytogenetic abnormalities in multiple myeloma: association with disease characteristics and treatment response
Authors: Abdallah Nadine , Rajkumar S. Vincent , Greipp Patricia , Kapoor Prashant , Gertz Morie A. , Dispenzieri Angela , Baughn Linda B. , Lacy Martha Q. , Hayman Suzanne R. , Buadi Francis K. , Dingli David , Go Ronald S. , Hwa Yi L. , Fonder Amie , Hobbs Miriam , Lin Yi , Leung Nelson , Kourelis Taxiarchis , Warsame Rahma , Siddiqui Mustaqeem , Lust John , Kyle Robert A. , Bergsagel Leif , Ketterling Rhett , Kumar Shaji K. 
Published: August 2020

AbstractCytogenetic abnormalities are found in most multiple myeloma (MM) patients. Although their prognostic value has been well studied, there are limited data on the association of primary cytogenetic abnormalities with disease characteristics and treatment response. This study was designed to evaluate these associations. This is a retrospective study including 2027 Mayo Clinic patients diagnosed with MM between February 2004 and February 2018 who had cytogenetic testing by FISH at diagnosis. Translocations t(4;14), t(14;16), t(6;14), and t(14;20) were associated with anemia, beta2microglobulin >5.5 µg/ml and ≥50% bone marrow plasma cells; t(4;14) was associated with higher serum monoclonal protein and plasma cell proliferation. Overall response rate to proteasome inhibitor (PI)-based treatment was higher for IgH translocations compared to trisomies (83% vs. 71%, P = 0.002), but was higher for trisomies with immunomodulatory drug (IMiD)-based treatment (87% vs. 75%, P < 0.001). Time to next treatment was longer with trisomies than IgH translocation with IMiD-based (32.1 vs. 18.4 months, P < 0.001) and PI + IMiD-based (44.0 vs. 27.4 months, P = 0.003) treatments. Outcomes were superior with PI + IMiD combinations in all groups. Our results show that t(4;14), t(14;16), t(6;14), and t(14;20) are associated with high-risk disease characteristics, and IgH translocations and trisomies may be associated with better responses to PIs and IMiDs, respectively.
Plasma-based early screening and monitoring of EGFR mutations in NSCLC patients by a 3-color digital PCR assay
Authors: Song Xiang , Gong Jian , Zhang Xiaoling , Feng Xiaoyan , Huang Hui , Gao Min , Chu Li 
Published: August 2020

Abstract
Background
Noninvasive plasma-based detection of EGFR mutations using digital PCR promises a fast, sensitive and reliable approach to predicting the efficiency of EGFR-TKI. However, the low throughput and high cost of digital PCR restricts its clinical application.

Methods
We designed a digital PCR assay, which can simultaneously detect 39 mutations of exons 18–21 of the EGFR gene. To assess overall performance, retrospective FFPE tissues from 30 NSCLC patients and plasma from 33 NSCLC patients were collected and analysed.

Results
The LoD of the EGFR mutations was as low as 0.308 copies/μL, and the linear correlation between the detected and expected values at different concentrations (0.01–10%) was low as well. Compared to ARMS-PCR in FFPE, the accuracy values of the dEGFR39 assay in plasma from 33 patients was 87.88% (29/33, 95% CI 72.67–95.18%). While monitoring the 33 patients, the EGFR mutation load as assessed by dEGFR39 was associated with the objective response to treatment. Thirteen samples from eight patients were identified by dEGFR39 to harbour the T790M mutation over time; of these patients, only nine (69%) were detected using SuperARMS.

Conclusion
Our results indicate that dEGFR39 assay is reliable, sensitive and cost-efficient. This method is beneficial for profiling EGFR mutations for precision therapy and prognosis after TKI treatment, especially in patients with insufficient tissue biopsy samples.