bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and Characterization of Staphylococcus Aureus Phage and Its Anti-Biofilm Activity Individually or Collaborative with Streptomycin
Authors: Jiang Liming , Zheng Rui 
Published: May 2020

Abstract
Background: Staphylococcus aureus was a widespread of Gram-positive pathogen bacteria which causes a wide range of symptoms. Bacteria biofilm was the multicellular community of microorganisms that attached to non-biological and biological surfaces. Method: Here, we aimed to isolation and characterization of S. aureus phage and research its bactericidal activity that individually or collaborative with streptomycin.Results: In this study, virulent phage WX was isolated from slaughter house in Jiangsu, China. It’s belonged to the Siphoviridae family and optimal growth temperature was 37 ℃, the pH of optimal preservation buffer was 6~7, optimal multiplicity of infection (MOI) was 0.01 and the genome size was 141, 342 bp. Phage WX has can sterilize most clinical strains of S. aureus which was isolated from clinical patients in the first people's hospital of Yunnan province laboratory. Streptomycin has better anti-biofilm effect than phage WX in low concentration culture of bacteria, nonetheless, phage WX has better anti-biofilm effect than streptomycin in high concentration culture of bacteria. Collaboration of phage WX and streptomycin have better anti-biofilm effect than alone of WX or streptomycin in low concentration culture of bacteria and phage WX have better anti-biofilm effect than streptomycin in high concentration culture of bacteria. Conclusion: The data of this study provided a strong evidence of application phage for reduce the growth of S. aureus biofilm, this study was important for clinic and replace antibiotics in some extent.
Stand structural diversity and species with leaf nitrogen conservative strategy drive aboveground carbon storage in tropical old-growth forests
Authors: Wang Genzhu , Liu Yuguo , Wu Xiuqin , Pang Danbo , Yang Xiao , Hussain Azfar , Zhou Jinxing 
Published: May 2020

Abstract
Background: Tropical old-growth forest ecosystems are essential for global carbon regulation. Even there are mounting evidences for the significance of species and functional composition, stand structure and elevation gradients on aboveground carbon storage, the relative strengths of these drivers and whether elevation effects via biotic factors are not clear. Furthermore, the mechanisms (the mass-ratio hypothesis or niche complementarity hypothesis) are still poorly understood.Methods: We analyzed aboveground carbon storage, species diversity, stand structural diversity, community-weighted mean (CWM) of functional traits and functional diversity (FDvar) using date from 56 old-growth forest communities with different elevation gradients in Dawei mountain of southwestern China. Multiple regression models were used to test the relative importance of the predictor variables and structural equation model was used to explore the direct and indirect influences on the aboveground carbon storage.Results: Our optimal multiple regression model show aboveground carbon storage is mostly affected by diameter at breast height (DBH) diversity, followed by FDvar of dry matter concentration in mature leaves and CWM nitrogen concentration in young leaves. The final structural equation model indicates elevation indirectly affected aboveground carbon storage via DBH diversity. The stand structural diversity, but not species diversity or functional diversity, enhanced aboveground carbon storage.Conclusions: Our results indicate mass-ratio and niche complementarity effect promote aboveground carbon storage simultaneously. The complex stand structure and species with leaf nitrogen conservative strategy were the crucial drivers of aboveground carbon storage in tropical old-growth forests.
Deciphering miRNAs involved in crosstalk between auxin and cold stress in Arabidopsis roots
Authors: Aslam Mohammad , Sugita Kenji , Qin Yuan , Rahman Abidur 
Published: May 2020

Abstract
The phytohormone auxin and microRNA-mediated regulation of gene expressions are key regulators for plant growth and development at both optimal and under low-temperature stress conditions. However, the mechanistic link between microRNA and auxin in regulating plant cold stress response remains elusive. To better understand the role of microRNA in the crosstalk between auxin and cold stress responses, we took advantage of the mutants of Arabidopsis thaliana with altered response to auxin transport and signal. Screening of the mutants for root growth recovery after cold stress at 4°C revealed that the auxin signaling mutant, solitary root 1 ( slr1; mutation in Aux/IAA14), shows a hypersensitive response to cold stress. Genome-wide expression analysis of miRNA in wild-type and slr1 mutant roots using next-generation deep sequencing revealed 180 known and 86 novel cold-responsive microRNAs. Cold stress also increased the abundance of 26nt-31nt small RNA population in slr1 compared with wild-type. Comparative analysis of microRNA expression shows differential expression of 13 miRNAs in slr1 compared with wild-type. Target gene expression analysis of one of the potential candidate miRNAs, miR169 revelaed the possible involvement of miR169- NF-YA module in the auxin-mediated cold stress response. Taken together, these results indicate that SLR/IAA14, a transcriptional repressor of auxin signaling, plays a crucial role in integrating miRNA in auxin and cold responses.
Identification of Mitochondrial-related Prognostic Biomarkers Associated with Metabolic Processes of Hepatocellular Carcinoma
Authors: Zhang Tao , Nie Yingli , Cai Kailin , Chen Xiangdong , li huili , Wang Jiliang 
Published: May 2020

Abstract
Background

Hepatocellular carcinoma (HCC) is one of the leading causes of tumor-associated deaths worldwide. Despite the great progress in early diagnosis and multidisciplinary tumor management, the long-term prognosis of HCC remains unfavorable. Currently, metabolic reprogramming is widely found during tumor development to satisfy the growth and proliferation of cancer cells. And several metabolic targets have been identified and used as cancer biomarkers. Liver and mitochondrion are the two hubs of metabolism in humans in the organic and cellular levels, respectively. Thus, identifying prognostic biomarkers in mitochondrial-related genes (Mito-RGs) that reflect the metabolic alteration of HCC could lead to better interventions for HCC patients.
Methods

mRNA expression profiles and clinicopathological data of hepatocellular carcinoma (HCC) were obtained from The Cancer Genome Atlas (TCGA) database and Mito-RGs were obtained from mSigDB database. Weighted gene coexpression network analysis (WGCNA) was used to identify the interesting gene module that associated with HCC prognosis. Based on a univariate and the least absolute shrinkage and selection operation (LASSO) Cox regression analyses, a Mito-RGs based classifier was established and used for predicting the overall survival (OS) in HCC patients. Receiver operating characteristic (ROC) curve and multivariate Cox regression analysis were used to evaluate the prognosis performance of our classifier. Gene Set Enrichment Analysis (GSEA), Gene Set Variation Analysis (GSVA) and Consensus ClusterPlus algorithms were used to identify metabolic pathways that were significantly different between the high and low-risk groups based on the risk score calculated by the classifier.
Results

We constructed a classifier containing 10 Mito-RGs (ACOT7, ADPRHL2, ATAD3A, BSG, FAM72A, PDK3, PDSS1, RAD51C, TOMM34, and TRMU) for predicting the prognosis of HCC. And the classifier is an independent prognostic signature in predicting HCC patients' survival. Besides, the metabolic processes about fatty acid, amino acid, and bile acid were significantly downregulated in high-risk group, which may play notable roles in the development and unfavorable prognosis of HCC.
Conclusion

The Mito-RGs based classifier could act as an independent biomarker for survival prediction of HCC patients. Moreover, our research may provide a novel method for HCC metabolic therapy based on the modulation of mitochondrial function.
KLF5-mediated Eppk1 expression promotes cell proliferation in cervical cancer via the p38 signaling pathway
Authors: Pan Zhe , Liu Xiao , Chang Quan , Zhang Jin-jin , Hua Na , Li Guo-Hua , Ma Dong 
Published: May 2020

Abstract
Background: Epiplakin1 (Eppk1) is part of the EGF signal and is involved in cytoskeleton reorganization and cell proliferation. However, the role of Eppk1 in cervical cancer remains unknown.Objective: To determine the role of EPPK1 on cell proliferation in cervical cancer.Methods: The expression of Eppk1 and KLF5 as well as their correlation were assessed by RNA-seq, qRT-PCR, TCGA database and immunofluorescence staining. In CC cell lines, adenovirus-mediated overexpression or knockdown of KLF5 and Eppk1 as well as corresponding assessment of cell proliferation and signaling were determined by western blot and CCK8 experiments. Assays of lucifase reporter gene and CHIP were used to investigate mechanism between KLF5 and Eppk1.Results: Eppk1 expression was markedly in CC tissues and cell lines companied by KLF5 upregulation. The results of immunofluorescence staining further showed that the increased expression of Eppk1 and KLF5 correlated with progression of cervical tumorigenesis. Overexpression of KLF5 significantly increased Eppk1 expression at transcription and translation levels. Conversely, the knockdown of KLF5 by siRNA against KLF5 decreased Eppk1 expression. Mechanical studies showed that KLF5 activated Eppk1 transcription by direct binding to the Eppk1 promoter. Gain- and loss-of-function experiments showed that KLF5 promoted cell proliferation in Hela by upregulating Eppk1 expression. Moreover, KLF5-mediated the activation of EGFR and p38 signaling significantly decreased after Eppk1 knockdown companied with reduction of proliferating activity, suggesting that Eppk1 lies upstream of p38 signaling affecting cell proliferation in CC. Finally, the expression of Eppk1 positively correlated with tumor size.Conclusions: Eppk1 may be an effective therapeutic target on affecting EGFR-associated p38 signaling pathway and cell proliferation in cervical cancer.
Overexpression of IL-8 in patients with abdominal tumor after radiotherapy leads to ureteral obstructive renal dysfunction: a case-control study
Authors: Bai Liang , Luo Li , Gao Weicheng , Bu Chenfeng , Li Jun , Liu Huang 
Published: May 2020

Abstract
Purpose: To analyze the expression of interleukin-8 in patients with post-renal insufficiency after radiotherapy of abdominal tumors.Methods: 60 patients (experimental group) with post-renal insufficiency and 30 patients (control group) without renal insufficiency after the radiotherapy for abdominal tumors were collected for case-control study. Serum interleukin-8 levels were measured before and after the cystoscopy from September 2017 to March 2019, and the relationship between interleukin-8 levels and serum creatinine, and urea nitrogen, and hydronephrosis was analyzed.Results: The level of serum interleukin-8 levels were significantly increased in patients with post-renal insufficiency after radiotherapy for abdominal tumors(P<0.05).After treatment, the level of interleukin-8 in the experimental group was significantly lower than that before the treatment (P<0.05). The expression of interleukin-8 was positively correlated with serum creatinine (r=0.297, P=0.004), but not with urea nitrogen level and hydronephrosis (r1=-0.055, P1=0.605; r2=0.116, P2=0.277). Cox proportional risk regression model showed that interleukin-8 was a high risk factor for the occurrence of post-renal insufficiency (R2=0.826, P<0.05).Conclusion: Interleukin-8 is helpful to judge the renal function of patients with abdominal tumors after radiotherapy, In the early stage of renal insufficiency, it will overexpresses, and its evaluation is better than urea nitrogen.
Chromatin structure changes in Daphnia populations upon exposure to environmental cues – or – The discovery of Wolterecks “Matrix”
Authors: AUGUSTO Ronaldo de Carvalho , Minoda Aki , Rey Oliver , Cosseau Celine , Chaparro Cristian , Vidal-Dupiol Jeremie , Allienne Jean-Francois , Duval David , Pinaud Silvain , Tönges Sina , Andriantsoa Ranja , Luquet Emilien , Aubret Fabien , Sow Mamadou Dia , David Patrice , Thomson Vicki , Joly Dominique , Lima Mariana Gomes , Federico Deboraj , Danchin Etienne , Grunau Christoph 
Published: May 2020

Abstract
Background: This study therefore describes the classical experimental system postulated by Richard Woltereck 100 years ago: the adaptive morphological phenotypic plasticity of daphnia . Phenotypic plasticity is an important feature of biological systems that is likely to play a major role in the adaptation of organisms exposed to an environmental stimulus and it is increasingly related to epigenetic mechanisms. Several studies have started to identify the epigenetic basis of phenotypic plasticity of daphnia including non-coding RNAs, covalent modifications at the histone tails and DNA methylation however no study has yet investigated those effect on the genome-wide chromatin structure. The aim of this work was to study for the first time the overall genome-wide chromatin structure of Daphnia pulex in the context of the iconic complex defense response to predation. We developed a robust and rapid ATAC-seq technique that allows for analyzing chromatin of individual daphnia and show here (i) that this technique can be used with minimal expertise in molecular biology, and (ii) we used it to identify open chromatin structure in daphnia exposed to different environmental cues.Results: Our results encouraged the expected induction of anti-predatory morphological changes in the stress treatment was significantly higher than that of daphnia from the control treatment. The developed ATAC-seq technique can be used to characterize chromatin structures of individuals even those that are small and thus with few biological material, making it possible to determine epigenetic polymorphisms relatively easily and at reasonable cost in full populations. In addition, we deliver evidence that chromatin structure changes upon stimuli from the environment.Conclusion: We report here an extremely fast and straightforward method to map the chromatin status of individuals using small amounts of input biological material. W e show here that changes in the environment, such as predator presence the chromatin structure is profoundly reorganised confirming Woltereck ’s classical postulate.
Acutely Altered Mental Status as the Main Clinical Presentation of Multiple Strokes in Critically Ill Patients With COVID-19.
Authors: Díaz-Pérez Carolina , Ramos Carmen , López-Cruz Alberto , Olmedo José Muñoz , González Jimena Lázaro , de Vega-Ríos Enrique , González-Ávila Carmen , Hervás Carlos , Trillo Santiago , Vivancos José 
Published: May 2020

Abstract
Background and aims:Cerebral infarction in COVID-19 patients might be associated with a hypercoagulable state related to a systemic inflammatory response. Its diagnosis might be challenging. We present two critically ill patients with COVID-19 who presented acutely altered mental status as the main manifestation of multiple strokes.Methods:Clinical presentation and diagnostic work-up of the patients.Results:Two patients in their sixties were hospitalized with a bilateral pneumonia COVID-19. They developed respiratory failure and were admitted to ICU for mechanical ventilation and intense medical treatment. They were started on low-molecular-weight heparin since admission. Their laboratory results showed lymphopenia and increased levels of C-reactive protein and D-dimer. Case 1 developed hypofibrinogenemia and presented several cutaneous lesions with biopsy features of thrombotic vasculopathy. Case 2 was performed a CT pulmonary angiogram at ICU showing a bilateral pulmonary embolism. When waking up, both patients were conscious but with a remarkable global altered mental status without focal neurological deficits. A brain MRI revealed multiple acute bilateral ischemic lesions with areas of hemorrhagic transformation in both patients (Case 1: affecting the left frontal and temporal lobes and both occipital lobes; Case 2: affecting both frontal and left occipital lobes). Cardioembolic source and acquired antiphospholipid syndrome were ruled out. COVID-19-associated coagulopathy was suspected as the possible main etiology of the strokes.Conclusion:Acutely altered mental status might be the main manifestation of multiple brain infarctions in critically ill COVID-19 patients. It should be specially considered in those with suspected COVID-19-associated coagulopathy. Full-dose anticoagulation and clinical-radiological monitoring might reduce their neurological consequences.
Leakage reduction in WDNs through optimal setting of PATs with a derivative-free optimizer
Authors: Cimorelli Luigi , D'Aniello Andrea , Cozzolino Luca , Pianese Domenico 
Published: May 2020

Abstract
Excessive pressure in water distribution networks (WDNs) may lead to undesirable effects, such as increased pipe failure rate and leakages. Pressure management (PM) techniques are indeed attractive to address these issues, reducing energy and water losses. Among the most recent PM techniques, pumps working as turbines (PATs) can be employed to both control pressure and recover energy. However, finding the best location, setting, and number of PATs to maximize both leakage reduction and energy production within a WDN is particularly challenging due to the severe nonlinearity of the problem and the large number of decision variables. To address the setting problem, a promising derivative-free nonlinear programming method is herein presented. The proposed method, modified to account for bound-type constraints, is capable of finding the optimal setting of a chosen number of PATs, given their position, direction, and machine type (characteristic curve), accounting for both energy and saved water volumes costs. In addition, this method is also able to establish whether the installed PATs must be bypassed or not. The proposed method capabilities are tested on a hypothetical complex WDN taken from the literature.
Estilo de vida dos trabalhadores da saúde atuantes no norte de Minas Gerais
Authors: Rocha Suelen Ferreira , Silva Carla Silvana de Oliveira e , Carrasco Viviane , Andrade João Marcus Oliveira , De Almeida Emerson Willian Santos , Junior Renê Ferreira da Silva , Dias Orlene Veloso 
Published: May 2020

Resumo Objetivo: analisar o estilo de vida dos trabalhadores da saúde que prestam assistência direta a pacientes críticos/crônicos. Métodos: estudo transversal realizado com 235 trabalhadores da área da saúde que atuam nos serviços hospitalares referência em oncologia, nefrologia, terapia intensiva neonatal e pronto socorro do Norte de Minas Gerais. Foi utilizado instrumento autoaplicável, com questões referentes as características sociodemográficas, de formação e ocupacionais dos participantes, bem como questionário denominado Estilo de Vida Fantástico. Os dados foram tabulados e analisados por meio de software estatístico SPSS (Statistical Package Social Science), versão 20.0. Resultados: o valor médio global do estilo de vida foi de 72,38 pontos, o qual prediz em um estilo de vida “Muito bom”. Quanto as categorias agrupadas 17 (7,2%) trabalhadores apresentaram estilo de vida pouco saudável, e os domínios do questionário Fantástico que alcançaram as menores médias foram o de atividade física, seguido pelo tipo de comportamento e nutrição, estes que mais requerem mudanças. Conclusão: os trabalhadores da saúde apresentaram um estilo de vida muito bom e o Técnico de Enfermagem apresentou o estilo de vida menos saudável. Palavras-chave: estilo de vida; saúde do trabalhador; ambiente de trabalho.
Risk Models and Scores in Patients with Peripheral Artery Disease and Chronic Limb-threatening Ischemia: A Comprehensive Review
Authors: Kokkinidis Damianos G. , Katamreddy Adarsh , Giannopoulos Stefanos , Schizas Dimitrios , Georgopoulos Sotirios , Liakakos Theodore , Armstrong Ehrin J. , Bakoyiannis Christos 
Published: May 2020

:
Peripheral artery disease (PAD) affects more than 200 million patients worldwide and chronic limbthreatening
ischemia (CLTI) is the most advanced stage of PAD with very high morbidity and mortality rates.
Cardiovascular medicine is trending towards a more personalized approach where each individual patient will
be managed according to specific risk factors, disease characteristics, expectations related to their disease and
individualized assessment of potential outcomes. For this reason, a number of risk models and scores have
been developed during the last few years. Our aim in this comprehensive review article is to provide an overview
of selected risk models and scores for patients with PAD and CLTI. Given that some of the published
scores were of low quality (minimal discriminatory ability), we included scores that were already externally
validated or scores that had promising initial findings.

:
Available scoring systems were grouped in the five following categories according to their utility: i) scores that
can detect asymptomatic patients who should be screened for PAD, ii) scores for assessment of functional status
and quality of life in patients with PAD, iii) scores assessing risk for amputation and other major adverse limb
events among patients with CLTI, iv) scores for the optimal revascularization strategy in each patient and
scores predicting successful procedural outcomes; v) scores predicting short or long-term cardiovascular and
limb related outcomes after either revascularization or at least angiographic assessment. Limitations of available
scoring systems include development and validation in specific populations, lack of external validation (for
some of them) and also lack of synchrony with current era endovascular technology. However, with further optimization
of current scores and the development of new scores, the field of PAD and CLI can be transitioned to
a personalized medicine approach.
Highly Efficient Ultrasound Promoted Synthesis of 2-phenylquinoxaline in Glycerol- Water
Authors: Shaikh Mujahed , Wagare Devendra , Sonone Ashvini , Durrani Ayesha 
Published: May 2020

Introduction:
Quinoxalines show diversified applications in the field of medicinal chemistry.

Materials and Methods:
Therefore, we have designed a highly efficient, environmentally benign and one-pot
protocol for the synthesis of 2-phenylquinoxaline from the reaction of Acetophenone, N-bromosuccinimide and
1,2-phenylenediamine under ultrasound irradiation in glycerol-water.

Results and Discussion:
We observed that, although the reaction efficiently completed in all of these solvents,
the use of glycerol-water with different ratios gives consistently higher yields (89–94%) and decreases reaction
times.

Conclusion:
The main advantages of this protocol are that it is a green method, avoids the use of toxic
catalysts and volatile organic medium and the product is obtained with excellent yield.