bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating effects of preoperative inflammatory biomarkers on predicting survival outcomes of peripheral intrahepatic cholangiocarcinoma after curative resection.
Authors: Zhang Zeyu , Zhou Yufan , Hu Kuan , Huang Yun 
Published: September 2020

Abstract
Introduction: Intrahepatic cholangiocarcinoma (ICC) stands as the second most common malignant tumor in liver with poor patient prognosis. Increasing evidences have shown that inflammation plays a significant role in tumor progression, angiogenesis and metastasis. However, the prognosis significance of inflammatory biomarkers on recurrence-free survival (RFS) and overall survival (OS) in peripheral ICC patients is poorly recognized. Methods: Peripheral ICC patients who underwent curative hepatectomy and diagnosed pathologically were retrospectively analyzed. Inflammatory biomarkers, including neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), lymphocyte-to-monocyte ratio (LMR), systemic immune-inflammation index (SII), were investigated. Results: Receiver operating characteristic (ROC) curves showed no significance in NLR, PLR and LMR in RFS and OS, while significant results were shown on SII in both RFS (P=0.035) and OS (P=0.034) with areas under ROC curve as 0.63 (95%CI: 0.52-0.74) and 0.62 (95%CI: 0.51-0.72), respectively. Kaplan-Meier curves revealed statistically significant better survival data in SII-low groups on both RFS (P<0.001) and OS (P<0.001). The univariate and multivariate analyses revealed that higher level of SII was independently associated with both poorer RFS time and OS time. However, no significant result was shown on NLR, PLR or LMR. Conclusion: SII is an effective prognostic factor for predicting the prognosis of peripheral ICC patient undergone curative hepatectomy, while NLR, PLR and LMR are not associated with clinical outcomes of these patients.
Mainz Foot-school: a course concept for prevention and treatment of foot disorders
Authors: Betz Martin , Konradi Jürgen , Betz Ulrich , Drees Philipp 
Published: September 2020

Abstract
Background: Physiotherapy offers an active approach to prevent and treat foot dysfunction, but it is currently rarely used. A Spiraldynamik®-inspired 6‒8-h educational program, called “Mainzer Fußschule” (Mainz Foot-school, MFS), was established to offer a framework for effective implementation. Elements of the courses are: knowledge transfer about anatomy and function, perception training, mobilization, as well as strengthening and coordination. In order to evaluate opinions of former participants about the course format and their subjective effects of course participation, we collected patient-reported outcomes (PROs).Methods: In mid-2018, we conducted a retrospective, monocentric, questionnaire-based cohort study. The online-questionnaire comprised 23-items. All MFS attendees in 2015‒2017 who were contactable via e-mail (522; 90.3%) were approached. Finally, 350 completed questionnaires (67%) were returned.Results: The overall framework of the course was evaluated very positively by most participants, with 94.8% expressing a positive opinion about the course format, 97.2 % about the theory vs. practice ratio, and 97.2% about the information content. PROs regarding the offering were equally positive, with 84.3 % stating that their feet are now used and treated differently, and 63,9 % that they still perform exercises learned in the MFS. Furthermore, the majority (67.9 %) believes that the procedure had a positive effect on their existing foot problem. Feedback on the various other examined aspects was similarly positive.Conclusions: The courses were rated positively by the majority of former participants, both in terms of format and subjective effects. Thus the MFS is a promising therapy option that should be included in an extended treatment concept with individual orthopedic examination and therapy options. Our results should be verified in a multicenter, randomized controlled study.Trial registration: The Trial was approved by the ethical review committee of the Rhineland-Palatinate state chamber of physicians (18.04.2018). International Clinical Trials Registry Platform (ICTRP), DRKS00013890. Registered 11 May 2018, http://apps.who.int/trialsearch/Trial2.aspx?TrialID=DRKS00013890.
Maternal and Perinatal Outcomes in Women with Eclampsia by Mode of delivery at Riley Mother Baby Hospital: A longitudinal case-series study
Authors: Poli Philippe Amubuomombe , Irene Koech MMED , Mogeni Richard , Mwangi Ann , Cheruiyot Andrew , Omenge Orang'o Elkanah 
Published: September 2020

Abstract
Background

Eclampsia, considered a serious complication of preeclampsia, remains a life-threatening condition among pregnant women. It accounts for 12% of maternal deaths and 16–31% of perinatal deaths worldwide. Most deaths from eclampsia occurred in resource-limited settings of sub-Saharan Africa. This study was performed to determine the optimum mode of delivery, as well as factors associated with the mode of delivery, in women admitted with eclampsia at Riley Mother and Baby Hospital.
Methods

This was a hospital-based longitudinal case-series study conducted at the largest and busiest obstetric unit of the tertiary hospital of western Kenya. Maternal and perinatal variables, such as age, parity, medications, initiation of labour, mode of delivery, admission to the intensive care unit, admission to the newborn care unit, organ injuries, and mortality, were analysed using the Statistical Package for the Social Sciences software version 20.0. Quantitative data were described using frequencies and percentages. The significance of the obtained results was judged at the 5% level. The chi-square test was used for categorical variables, and Fisher’s exact test or the Monte Carlo correction was used for correction of the chi-square test when more than 20% of the cells had an expected count of less than 5.
Results

During the study period, 53 patients diagnosed with eclampsia were treated and followed up to 6 weeks postpartum. There was zero maternal mortality; however, perinatal mortality was reported in 9.4%. Parity was statistically associated with an increased odds of adverse perinatal outcomes (p = 0.004, OR = 9.1, 95% CI = 2.0-40.8) and caesarean delivery (p = 0.020, OR = 4.7, 95% CI = 1.3–17.1). In addition, the induction of labour decreased the risk of adverse outcomes (p = 0.232, OR = 0.3, 95% CI = 0.1-2.0).
Conclusion

There is no benefit of emergency caesarean section for women with eclampsia. Instead, it increases the risk of perinatal adverse outcomes, including the risk of admission to the newborn unit and perinatal death.
The Effects of Contralateral Throwing Practice on Throwing Parameters, Isometric Strength, Grip Strength, and Shoulder Range of Motion in High School Baseball Athletes
Authors: Gheith Raad , Sharp Matthew H. , Stefan Matthew W. , Reber Dallen D. , LoDuca Salvatore , Lowery Ryan P. , Wilson Jacob M. 
Published: September 2020

Abstract
Background

Baseball throwing is typically done with one dominant arm and after years of throwing the skills becomes autonomous and improvement is slowed. Incorporating training of the non-dominant arm could induce a cross-education effect and assist in improving the dominant throwing arm. Therefore, the purpose of this study was to determine the effect of contralateral (CL) throwing practice on dominant arm throwing accuracy and other parameters in experienced baseball players.
Methods

16 male high school baseball players participated in contralateral throwing practice. Each participant was randomly assigned to either contralateral (CL) or control (CON) following a pre-test. Sessions consisted of 30 non-dominant arm throws from a randomized location to a standard catch net, twice a week for 4 weeks. Following the 4 weeks of training, participants were post-tested to determine the effects of the contralateral throwing program. Accuracy percentage, throwing velocity, Accuracy:Velocity ratio, and non-specific throwing parameters were collected to determine the effect of the contralateral throwing program.
Results

For dominant side throwing accuracy %, significant pre- to post-testing differences were demonstrated in the CL condition (p < 0.05, Pre = 47.4, Post = 54.9). Accuracy:Velocity ratio was statistically significant in the CL condition in pre- to post-test (p < 0.05, Pre = 0.71, Post = 0.86). There were no significant differences detected in non-throwing specific parameters.
Conclusion

Contralateral throwing practice improved accuracy and Accuracy:Velocity ratio in the dominant arm of the CL group. Use of a contralateral throwing program can be used to improve performance in a well learned, complex, open skill such as baseball throwing.
A new hybrid record linkage process to render epidemiological databases interoperable: application to the GEMO and GENEPSO studies involving BRCA1 and BRCA2 mutation carriers
Authors: JIAO YUE , Lesueur Fabienne , Azencott Chloé-Agathe , Laurent Maïté , Mebirouk Noura , Laborde Lilian , Beauvallet Juana , Dondon Marie-Gabrielle , Eon-Marchais Séverine , Laugé Anthony , Collaborators GEMO Study , Collaborators GENEPSO Study , Noguès Catherine , Andrieu Nadine , Stoppa-Lyonnet Dominique , Caputo Sandrine M. 
Published: September 2020

Abstract
BackgroundLinking independent sources of data related to same individuals enable innovative epidemiological and health studies but requires a robust record linkage approach. We describe a hybrid record linkage process to link databases from two independent ongoing national studies, GEMO (Genetic Modifiers of BRCA1 and BRCA2), which focuses on the identification of genetic factors modifying cancer risk of BRCA1 and BRCA2 mutation carriers, and GENEPSO (prospective cohort of BRCAx mutation carriers), which focuses on environmental and lifestyle risk factors.MethodsTo identify the maximum individuals participating in the two studies but may not be registered by a common number, we combined Probabilistic Record Linkage (PRL) and supervised Machine Learning (ML). This combined linkage was named “PRL+ML”. We built the ML model using a first version of the two databases as a training dataset on which matching status was assigned by PRL followed manual review. ResultsThe Random Forest (RF) algorithm showed a highest sensitivity (0.985) among six widely used ML algorithms: RF, Bagged trees, AdaBoost, Support Vector Machine, Neural Network.Therefore, RF was selected to build the ML model since our goal was to identify the maximum of true matches. Our combined linkage PRL+ML showed a higher sensitivity (range 0.988-0.992) than either PRL (range 0.916-0.991) or ML (0.981) alone. It identified 2,068 individuals participating in both GEMO (6,375 participants) and GENEPSO (4,925 participants).ConclusionsOur hybrid linkage process represents an efficient tool for linking GEMO and GENEPSO. It may be generalizable to other epidemiological studies involving other databases and registries.
Quantification and Confirmation of Zearalenone Using a LC-MS/MS QTRAP System in Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes.
Authors: lv Shencong , Wu Xiaoqiong , Zhu Guoying , Guan Jian , Yan Yong , Ge Miaohua 
Published: September 2020

Abstract
Background: A simple, rapid, and efficient liquid chromatography tandem mass spectrometry (LC–MS/MS) method, operated in electrospray ionization (ESI) and quadrupole linear ion trap modes, has been developed for the identification and structural characterization of zearalenone (ZEN) in corn oil. Methods: Samples (5 g) were extracted with acetonitrile/water (80:20, v/v). After centrifugation and dilution, the extracts were separated on a C18 analytical column by gradient elution (acetonitrile/water) and analyzed by UPLC–MS/MS. Enhanced product ion mode was used for qualitative analysis, while multiple reaction monitoring mode was used for quantitative analysis. Results: Calibration curve showed good linearity with correlation coefficients (r) higher than 0.995. Limit of detection was determined to be below 0.20 μg kg-1 for ZEN. The recovery for ZEN was in the acceptable range of 86.6% to 97.2%. 82.4 % of the samples were found to contain ZEN among the 51 samples.Conclusion: The sample pretreatment and LC–MS methods developed in this research, from a convenience and analysis time perspective, are simple, efficient, cheaper, and less time-consuming than existing methods.
A Tablet ‐ Based Intervention for Activating Nursing Home Residents with Dementia: Results from a Cluster-Randomised Controlled Trial
Authors: O'Sullivan Julie Lorraine , Lech Sonia , Gellert Paul , Grittner Ulrike , Voigt-Antons Jan-Niklas , Möller Sebastian , Kuhlmey Adelheid , Nordheim Johanna 
Published: September 2020

Abstract
Background: Non-pharmacological interventions (NPI) can improve cognitive and non-cognitive symptoms in nursing home residents living with dementia. However, delivery of suitable NPI can be challenging in everyday nursing home settings. Internet and communication technologies (ICT) may be promising tools for supporting NPI delivery in nursing homes. Methods: A two-arm cluster-randomised controlled trial was conducted to investigate global and momentary effects of a novel ICT-based NPI for nursing home residents with dementia. Ten nursing homes were randomly allocated to the tablet-based intervention (TBI) or conventional activity sessions (CAS) group (each with five nursing homes) between April 2016 and May 2017. A total of N = 162 participants received either regular TBI (n = 80) or CAS (n = 82) over a period of eight weeks. Linear mixed models were used to analyse group differences regarding the primary outcome apathy (AES-I), and secondary outcomes quality of life (QOL-AD, QUALIDEM), neuropsychiatric (NPI-NH, psychotropic medication) and depressive symptoms (GDS). Ecological Momentary Assessments (EMA) of quality of life were also conducted in both groups before and after each activity session.Results: No significant group difference in the change of apathy (AES-I score, primary outcome) was found post intervention (mean group difference: B = .19; 95% CI: -3.90 to 4.28, p = .93). Regarding secondary outcomes, a reduction of psychotropic medication was found for TBI compared to CAS (B = .42; 95% CI: .15 to .69, p < .01). Further analyses revealed a post-intervention improvement of informant-rated quality of life across both groups (B = 3.69; 95% CI: .68 to 6.69, p = .02). Analysis of EMA also rendered short-term post-session improvements of quality of life in the CAS group (B = .43; 95% CI: .30 to .57, p < .001). Conclusions: These findings suggest that NPI involving individually tailored activities have a beneficial impact on quality of life in nursing home residents with dementia. Although we found no clear advantage of TBI compared to CAS, ICT have the potential to support NPI delivery and facilitate regular assessments of fluctuating momentary states in nursing home residents with dementia. Trial registration: The trial was retrospectively registered with the ISRCTN registry (Trial registration number: ISRCTN98947160) on 01/09/2016 http://www.isrctn.com/ISRCTN98947160.
Annotation of Gene Alteration in Amniotic Fluid Cell Free RNA Transcriptome via Weighted Gene Co-expression Network Analysis
Authors: Yang Shufa , Yin Chenghong , Liu Yan , Zhuang Taifeng 
Published: September 2020

Abstract
BackgroundHuman amniotic fluid (AF) cells are commonly used in prenatal diagnosis. The AF cell-free mRNA (cfRNA) derived from necrotic or apoptotic cells of fetus may provide feasible marker of organ development and fetal malformation. Analysis of gene co-expression network will help to annotate AF cfRNA gene variations in fetal diseases.MethodsDatasets of amniotic fluid free RNA were downloaded from the Gene Expression Omnibus database. Co-expressed modules based on normal fetus AF cfRNA transcriptome were established via Weighted Gene Co-expression Network Analysis. The relationship between modules and tissues were set up via tissue-specific gene expression analysis of genes in modules. Differential expressed genes in Down syndrome (DS), Edwards syndrome (ES), and Turner syndrome (TS) were analyzed via linear models implemented in the limma package. Gene Ontology (GO) analysis of modular specific differential expressed genes for these three syndrome fetus was performed separately. Based on relationship of GO terms, GO graph of all enriched GO terms were constructed. The associated enriched GO terms in GO graph were considered as the same subset. Numbers of GO terms of the same subset with diseases and modules were calculated.ResultsA total of 22 co-expressed modules were constructed. Most of co-expressed module eigengenes showed no correlation with gestation weeks. Probe sets with higher expression values showed significant clustering tendencies. Dominant tissues and modules were different for different modules and tissues respectively through analysis of tissue-specific genes distribution in modules. The total numbers of enriched GO terms in six major modules were related to diseases severity for ES, DS, and TS. Disease-specific modules for ES, DS, and TS were detected in relation to biological processes through GO analysis. Differential expressed genes in disease-specific module were identified for DS, ES, and TS.ConclusionsThe gene co-expression network for functional classification provides a potential tool for annotating AF cfRNA variations in fetal diseases.
Concurrent cerebral arterial and venous sinus thrombosis revealing celiac disease - A case report and literature review
Authors: Alhosain Dalia , Kouba Lamia 
Published: September 2020

Abstract
Background: Celiac disease is an autoimmune condition characterized by an inappropriate immune reaction against gluten. It classically presents as chronic diarrhea, bloating, and nausea in addition to malabsorption symptoms such as weight loss and micronutrient deficiency. We report the first case of coinciding cerebral infarction and venous sinus thrombosis unveiling the diagnosis of celiac disease.Case Presentation: A 40-year old female patient with a four-day history of severe diarrhea presented with right hemiplegia and altered mental status. Imaging revealed left middle cerebral artery occlusion and left transverse and sigmoid venous sinus thrombosis, along with left jugular vein thrombosis. Her laboratory evaluation was notable for profound iron deficiency anemia, thrombocytosis, and hyperhomocysteinemia. Her positive anti-tissue transglutaminase IgA antibodies and ensuing duodenal biopsy confirmed the diagnosis of celiac disease.Conclusions: Celiac disease has a wide range of intestinal and extra-intestinal manifestations and can present with thrombotic events in young patients with iron deficiency and hyperhomocysteinemia.
Examining the Determinants of Contraceptive Use in Northern Nigeria
Authors: Aduloju-Ajijola Natasha M , Idogho Omokhudu , Yusuf Farouk , Muhammed Fatima , Anyanti Jennifer 
Published: September 2020

Abstract
Background: The Northern part of Nigeria, has higher rates of child marriage, lower levels of contraceptive use, lower rates of antenatal care, fewer births delivered in a health facility, and higher total fertility rates and adolescent fertility rates than Southern regions. The purpose of this study is to identify predictors of current and intentions to use contraception, among young women of Northern Nigeria. Methods: The data analyzed in this study are from 2018 Nigeria Demographic and Health Survey, part of the Demographic and Health Survey by USAID. Results: Participants (N = 10066) ranged in age from 15 to 24 (mean = 19, sd = 2.76) from the three northern regions of Nigeria, primarily lived in rural areas (68.7%, n = 7107). We found that, 83.3 percent of the sample had heard of modern contraceptive methods, though only 3.6 (n = 357.2) were currently using a modern contraceptive method, and 43.4 (n = 4286.4) intended to use them. Conclusion: The primary predictors of contraceptive use include literacy, wealth, educational levels, being the decision maker regarding contraceptives, age of first sex, wanting more children, and decisional autonomy in the home. Since the literacy is low, interventions should utilize pictures and radio programs, traditional and religious leaders, and incorporate men.
Large carbon sink potential of Amazonian Secondary Forests to mitigate climate change
Authors: Heinrich Viola , Dalagnol Ricardo , Cassol Henrique , Rosan Thais , Almeida Catherine Torres de , Junior Celso H. L. Silva , Campanharo Wesley , House Joanna , Sitch Stephen , Hales Tristram , Adami Marcos , Anderson Liana , Aragão Luiz 
Published: September 2020

Abstract
Secondary forests (SF) have a large climate mitigation potential, given their ability to sequester carbon up to 20 times faster than old-growth forests. Environmental variability and anthropogenic disturbances lead to uncertainties in estimating spatial patterns of SF carbon sequestration rates. Here we quantify the influence of environmental and disturbance drivers on the rate and spatial patterns of regrowth in the Brazilian Amazon, by integrating a 33-year land cover timeseries with a 2017 Aboveground Biomass dataset. Carbon sequestration rates of young Amazonian SF (<20 years old) are at least twice as high in the west (3.0±1.0 MgC ha-1 yr-1) than in the east (1.3±0.3 MgC ha-1 yr-1). Disturbances reduce SF regrowth rates by 8–50% (0.6 – 1.3 MgC ha-1 yr-1). We estimate the 2017 SF carbon stock to be 294 TgC, which could be 8% higher by avoiding fires and repeated deforestation. Maintaining the 2017 SF area has the potential to accumulate ~15 TgC yr-1 until 2030, contributing ~5% to Brazil’s 2030 net emissions reduction target. Supporting SF and old-growth forests conservation alongside the expansion of SF in deforested areas is therefore a viable nature-based climate mitigation solution.
LncRNA&nbsp;Profiling of Exosomes Derived From Staphylococcus Aureus-induced Bovine Mammary Epithelial Cell Lines MAC-T
Authors: Chen Yu , Jing Hongyuan , Chen Miaoyu , Liang Wan , Guo Mengyao 
Published: September 2020

Abstract
Background: Exosomes (carrying proteins, miRNA, lncRNA, etc.) play a vital role in participating in the occurrence and development of mastitis. LncRNA can play a variety of regulatory roles by combining with protein, RNA, and DNA. The expression of mRNA and lncRNA in exosomes derived from bovine mammary epithelial cells infected by S. aureus is rarely understood.Methods: We used ultracentrifugation to separate exosomes derived from S. aureus- stimulated MAC-T cells. After extracting exosomal RNA, RNA sequencing was performed. We screened differentially expressed genes, mRNA and lncRNA, and predicted their functions through KEGG and GO analysis. 6 mRNA and lncRNA were randomly selected for RT-qPCR verification.Results: Analysis of the sequencing results showed that there were 186 differentially expressed genes, 431 differentially expressed mRNAs and 19 differentially expressed lncRNAs in the exosomes derived from S. aureus-infected and non-infected MAC-T acells. By predicting lncRNA target genes, it was found that 19 differentially expressed lncRNAs all acted on multiple mRNAs in cis and trans. GO analysis revealed that differentially expressed genes and lncRNA target genes played significant roles in such metabolism, transmembrane transport, cellular response to DNA damage stimulus, response to cytokines. KEGG enrichment indicated that lncRNA target genes gathered in the TNF pathway, Notch pathway, phosphatidylinositol signaling system and p53 pathway. Conclusion: The data suggested that S. aureus induced changes in genes, mRNA, and lncRNA in exosomes secreted by MAC-T cells, which furtherly triggered other cells damage through exosomal delivery. This study would provide valuable resource for understanding the lncRNA information in exosomes derived from dairy cow mammary epithelial cells, and conduced to the study of S. aureus infection in dairy cow mammary glands.