bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ring Finger Protein 11 acts on ligand‐activated EGFR via the direct interaction with the UIM region of ANKRD13 protein family
Authors: Mattioni Anna , Boldt Karsten , Auciello Giulio , Komada Masayuki , Rappoport Joshua Z. , Ueffing Marius , Castagnoli Luisa , Cesareni Gianni , Santonico Elena 
Published: January 2020

RING finger protein 11 (RNF11) is an evolutionary conserved Really Interesting New Gene E3 ligase that is overexpressed in several human tumours. Although several reports have highlighted its involvement in crucial cellular processes, the mechanistic details underlying its function are still poorly understood. Utilizing stable isotope labelling by amino acids in culture (SILAC)‐based proteomics analysis, we identified 51 proteins that co‐immunoprecipitate with wild‐type RNF11 and/or with its catalytically inactive mutant. We focused our attention on the interaction of RNF11 with Ankyrin repeat domain‐containing protein 13 (ANKRD13)s family. Members of the ANKRD13 family contain ubiquitin‐interacting motifs (UIM) that recognize the Lys‐63‐linked ubiquitin (Ub) chains appended to Epidermal growth factor receptor (EGFR) soon after ligand binding. We show that ANKRD13A, ANKRD13B and ANKRD13D form a complex with RNF11 in vivo and that the UIMs are required for complex formation. However, at odds with the conventional UIM binding mode, Ub modification of RNF11 is not required for the interaction with ANKRD13 proteins. We also show that the interaction between ANKRD13A and RNF11 is modulated by the EGF stimulus and that a complex formed by ANKRD13A, RNF11 and activated EGFR is transiently assembled in the early phases of receptor endocytosis. Moreover, loss of function of the E3 ligases Itchy E3 ubiquitin‐protein ligase (ITCH) or RNF11, respectively, abrogates or increases the ubiquitination of endogenous ANKRD13A, affecting its ability to bind activated EGFR. We propose a model whereby the ANKRD13 proteins act as molecular scaffolds that promote the transient formation of a complex between the activated EGFR and the E3 ligases ITCH and RNF11. By regulating the ubiquitination status of ANKRD13A and consequently its endocytic adaptor function, RNF11 promotes sorting of the activated EGFR for lysosomal degradation.
Explaining regulatory autonomy in EU network sectors: Varieties of utility regulation?
Authors: Wassum Moritz , De Francesco Fabrizio 
Published: January 2020

AbstractThis article explains variation in the autonomy in the range of activities that European regulators perform. By focusing on 102 regulators of four network sectors (electricity, gas, telecom, and railways), we test for functional and institutional explanations. The findings indicate that the inclusion of institutional factors matters for our understanding of recent changes in the governance of European network sectors. Reforms toward the independent agency form of governance and the range of competencies granted to sector regulators seem to be shaped not only by international functional pressures but also by domestic institutional factors. Beyond the credibility hypothesis, we find that national governments grant less regulatory autonomy to utility regulators the more coordinated an economy is and the more veto players are present. On the contrary, common law countries are associated with higher levels of regulatory autonomy.
The varieties of banking regulation in the EU: An empirical analysis
Authors: Kudrna Zdenek 
Published: January 2020

AbstractThe EU strives to harmonize banking regulation, while its member states continue to insist on a degree of differentiation to accommodate national specifics. A new data set of national options and discretions (O&Ds) embedded in EU banking legislation facilitates systematic analysis of member states' policy preferences across multiple policy cycles. Its results suggest that states' O&D choices are related to their respective variety of banking capitalism. Coordinated and liberal market economies choose O&Ds to protect distinct subsets of regulatory parameters delaying full harmonization. Dependent market economies on the Eastern periphery use O&Ds to prevent the outflow of capital from foreign‐owned subsidiaries under their jurisdiction. These deep institutional roots suggest that many O&Ds will be carried over to the upcoming generation of EU banking legislation, despite the increased harmonization pressures in the banking union.
Endometrial intravascular thrombi are typically associated with shedding but may be the sentinel feature of an underlying thrombotic disorder
Authors: Stewart Colin J R 
Published: January 2020

AimsFollowing the identification of endometrial intravascular thrombi (IVT) as the presenting feature in a patient with antiphospholipid syndrome, additional biopsy specimens were reviewed to determine the frequency and histological associations of IVT in the endometrium.Methods and ResultsEighty‐five additional biopsies were reviewed including 44 consecutive cases demonstrating shedding changes (both menstrual and non‐cyclical), 30 cases without shedding (normal and non‐cyclical), and 11 cases in which unusually prominent IVT had been recorded upon initial histological assessment. In the shedding group, IVT were significantly more common in biopsies showing disordered proliferative endometrium (DPE, 4/7 cases) than normal menstrual appearances (4/22 cases), and organising vascular changes were seen only in the former. IVT in DPE cases were also commonly multifocal and sometimes involved abnormal ectatic vessels. None of the 30 non‐shedding cases demonstrated IVT. Eight of the 11 biopsies with prominent IVT demonstrated DPE and shedding but three cases demonstrated otherwise normal cyclical appearances.ConclusionsIVT may be seen occasionally in normal menstrual endometrium but are more characteristic of DPE, and organising vascular changes are suggestive of abnormal haemostasis. An underlying thrombotic disorder may be considered when endometrial IVT occur in the absence of shedding changes.
Gastric‐type gene expression and phenotype in non‐terminal respiratory unit type adenocarcinoma of the lung with invasive mucinous adenocarcinoma morphology
Authors: Koh Myoung Ju , Shin Dong Hoon , Lee So‐Jeong , Hwang Chung‐Su , Lee Hyun Jung , Kim Ahrong , Park Won Young , Lee Jung Hee , Choi Kyung Un , Kim Jee Yeon , Lee Chang Hun , Sol Mee Young 
Published: January 2020

AimsWe sought to determine if non‐terminal respiratory unit (TRU) type adenocarcinoma of lung with invasive mucinous adenocarcinoma (IMA) morphology shows gastric differentiation.Methods and resultsWe reviewed whole‐section images of 489 cases of lung adenocarcinoma from The Cancer Genome Atlas (TCGA). TCGA data were classified into 426 TRU type adenocarcinoma, 49 IMA and 14 unclassifiable. Their RNA sequencing data was analysed by DESeq2 and WGCNA R packages. Gene expression in patients’ samples was measured by NanoString assay. Overexpression of genes including REG4, TFF2, MUCL3, FER1L6, B3GALT5, ANXA10 was observed by TCGA analysis in IMA compared to TRU type adenocarcinoma. Many of these genes are those expressed in normal gastric glands and selected for NanoString experiment on 14 IMA and 10 TRU type adenocarcinoma cases. The expression of genes, including ANXA10, FER1L6, HNF4a, MUC5AC, REG4, TFF1, TFF2 and VSIGI, was increased> 15‐fold in IMA. Immunohistochemistry of ANXA10, TFF2 and FER1L6 performed on 31 IMA and 135 TRU type adenocarcinomas showed a predominant expression in IMA, but are not in TRU type adenocarcinoma.ConclusionOur results showed the level of genes expressed in stomach mucosa was increased in IMA compared to TRU type adenocarcinoma, supporting gastric differentiation of IMA. This finding may help the understanding of the pathogenesis of IMA and discovery of therapeutic targets.
Promoting spinel formation and growth for preparation of refractory materials from ferronickel slag
Authors: Gu Foquan , Peng Zhiwei , Zhang Yuanbo , Tang Huimin , Tian Weiguang , Lee Joonho , Rao Mingjun , Li Guanghui , Jiang Tao 
Published: January 2020

AbstractThe study provides a method for improving the quality of the refractory material prepared from ferronickel slag by promoting the spinel formation and growth in the slag which was sintered with sintered magnesia and chromium oxide in a broad sintering temperature range from 1200°C to 1500°C. According to the thermodynamic analysis, except for forsterite due to the addition of sintered magnesia, a number of high‐melting point spinel phases can also be formed in the presence of chromium oxide and this trend becomes more apparent with increasing sintering temperature, along with declined presence of low‐melting point clinopyroxene, mainly enstatite. This expectation was verified by conversion of a part of the original phase of ferronickel slag, olivine, to two main spinel phases, including magnesium aluminate spinel and donathite which was produced by the replacement of nontoxic Cr3+ ions with Fe3+ ions in the octahedral vacancies of magnesium chromate spinel. The formation and growth of these spinel phases were promoted by elevating temperature from 1200°C to 1500°C, which accelerated the transition of initially generated enstatite to a glassy phase, in favor of densification. The formation and growth of spinel during sintering contributed to high refractoriness and compressive strength of the resulting refractory materials
Structure, viscosity, and crystallization of glass melt from molten blast furnace slag
Authors: Zhang Wentao , He Feng , Xiao Yongli , Xie Mengqin , Li Fengxiang , Xie Junlin , Yang Hu , Li Zuhao 
Published: January 2020

AbstractMolten blast furnace slag (BF slag) was modified using liquid‐liquid mixing method for glass ceramics preparation, and the structure and viscosity of glass melt after modification were investigated. The BF slag proportion increased from 52.72 wt. % to 67.05 wt. %, and CaO/(SiO2 + Al2O3) also varied from 0.33 to 0.47. The structure of BF slag melt after modification was investigated using 29Si and 27Al MAS NMR spectra. With increasing CaO/(SiO2 + Al2O3), the amount of Q1 remarkably increased while that of Q2 decreased significantly. Specifically, when CaO/(SiO2 + Al2O3) was more than 0.38, Q3 disappeared in glass melt. The viscosity of glass melt with CaO/(SiO2 + Al2O3) of 0.47 exhibited a steep change at 1169°C, which was mainly caused by the sufficient crystallization of gehlenite confirmed by DTA cooling analysis, XRD, and FE‐SEM‐EDS analysis.
Workplace violence against emergency department nurses in Oman: a cross‐sectional multi‐institutional study
Authors: Al‐Maskari S.A. , Al‐Busaidi I.S. , Al‐Maskari M.A. 
Published: January 2020

AbstractAimTo examine the prevalence, characteristics and factors associated with workplace violence against emergency department nurses in Oman.BackgroundViolence against healthcare professionals, including nurses, is a global public health problem. Understanding the epidemiology of violence is crucial for the implementation of appropriate preventative and management policies.MethodsWe conducted a multi‐centre cross‐sectional study in February 2019 utilizing a widely employed self‐report questionnaire developed by the World Health Organization. We invited all emergency department nurses working in four governmental hospitals in Al‐Sharqiyah and Al‐Dakhiliyah Governorates of Oman to participate in the study.ResultsOf the 103 respondents, most were Omani (57.3%), female (73.8%), and 30 years or older (65%). The majority (87.4%) were exposed to violence in the past 12 months, and non‐physical violence (84.5%) was over fourfold greater than physical violence (18.4%). Most physical and non‐physical incidents took place during weekends (68.4% and 82.8%, respectively) and in the afternoon or night shifts (78.9‬% and 93.1%, respectively), and family members and visitors were the most common perpetrators of violence (62.6% physical and 66.7% non‐physical). Non‐Omani nurses (P = 0.03) and those with education less than a bachelor’s degree (P = 0.007) were less likely to experience physical violence.Discussion/ConclusionWorkplace violence is highly prevalent among emergency department nurses in Oman. Future studies should investigate violent incident reporting, and the causes and consequences of workplace violence on nurses’ psychological and physical well‐being, work functioning and the quality of care provided to patients.Implications for Nursing PolicyThe high prevalence of workplace violence is alarming and demonstrates the need for additional interventions to reduce violence and limit its consequences on healthcare professionals and patients’ quality of care. Findings from this study can be used to implement changes in existing anti‐violence policies.
Pedigree‐free quantitative genetic approach provides evidence for heritability of movement tactics in wild roe deer
Authors: Gervais Laura , Hewison Aidan J. M. , Morellet Nicolas , Bernard Maria , Merlet Joël , Cargnelutti Bruno , Chaval Yannick , Pujol Benoit , Quéméré Erwan 
Published: January 2020

AbstractAssessing the evolutionary potential of animal populations in the wild is crucial to understanding how they may respond to selection mediated by rapid environmental change (e.g. habitat loss and fragmentation). A growing number of studies have investigated the adaptive role of behaviour, but assessments of its genetic basis in a natural setting remain scarce. We combined intensive biologging technology with genome‐wide data and a pedigree‐free quantitative genetic approach to quantify repeatability, heritability and evolvability for a suite of behaviours related to the risk avoidance‐resource acquisition trade‐off in a wild roe deer (Capreolus capreolus) population inhabiting a heterogeneous, human‐dominated landscape. These traits, linked to the stress response, movement and space‐use behaviour, were all moderately to highly repeatable. Furthermore, the repeatable among‐individual component of variation in these traits was partly due to additive genetic variance, with heritability estimates ranging from 0.21 ± 0.08 to 0.70 ± 0.11 and evolvability ranging from 1.1% to 4.3%. Changes in the trait mean can therefore occur under hypothetical directional selection over just a few generations. To the best of our knowledge, this is the first empirical demonstration of additive genetic variation in space‐use behaviour in a free‐ranging population based on genomic relatedness data. We conclude that wild animal populations may have the potential to adjust their spatial behaviour to human‐driven environmental modifications through microevolutionary change.
Effects of short‐term heat stress on the growth and development of Bradysia cellarum and Bradysia impatiens
Authors: Gou Yuping , Guo Sufan , Wang Guang , Liu Changzhong 
Published: January 2020

AbstractBradysia cellarum Frey and Bradysia impatiens Johansen are major pests of vegetable crops, as well as edible mushrooms and ornamental plants, and damage to hosts resulting in economic losses. Temperatures above the optimum levels for these pests have been predicted to regulate their population growth during summer. The aim of the present study was to examine the effects of both heat stress and exposure time on the growth and development of eggs, larvae and pupae for two Bradysia species. The egg stage, egg hatching rate, 4th instar larval stage, pupation rate, pupal stage and adult emergence rate were observed after exposing at high temperatures of 34°C, 37°C and 40°C for 1, 2, 4 and 6 hr. The results showed that 34°C, 37°C and 40°C for 1‐, 2‐, 4‐ and 6‐hr exposure treatments prolonged the developmental stage of egg, 4th instar larva and pupa, while decreasing the egg hatching rate, pupation rate and adult emergence rate. This suggests that increasing temperature or prolonging exposure time to the heat stress could significantly affect insect survival, growth and development. Our study could provide an ecological basis for pests’ management strategy by using short‐term heat stress.
Using life stage‐structured matrix models to determine natural enemy:pest release ratios for augmentative biological control
Authors: Gontijo Lessando M. , Carvalho Rayana M. R. 
Published: January 2020

AbstractAugmentative biological control (ABC) is crucial for the integrated pest management (IPM) of greenhouse pests. The proper calculation of natural enemy:pest release ratio (NEPRR) stands out among the various challenges of ABC. Often, most NEPRR calculations only take into account the predation/parasitism efficiency of natural enemies, while disregarding pest population growth capacity, population structure and/or economic thresholds. In a model system consisting of Trichogramma pretiosum Riley parasitizing eggs of Tuta absoluta (Meyrick) on greenhouse tomatoes, we investigated whether a stage‐structured Lefkovitch matrix could be used to determine suitable NEPRRs. The results suggested that these matrix models can potentially provide more precise and realistic predictions of NEPRRs since they account for differential pest population growth over time as a response to natural enemy action. The matrix models could also allow us to choose an adaptable NEPRR based on parameters such as the period the grower wishes to keep the pest population below economic threshold.
Freezing ovarian fluid does not alter how it affects fish sperm swimming performance: creating a cryptic female choice ‘spice rack’ for use in split‐ejaculate experimentation
Authors: Purchase Craig F. , Rooke Anna C. 
Published: January 2020

AbstractCryptic female choice is often mediated chemically in external fertilizers by ovarian fluid (OF), which can change sperm swimming performance and bias paternity under sperm competition. Assessing cryptic female choice is hindered by the necessity of using fresh gametes and the short time window available to obtain diverse samples from wild animals. Using split‐ejaculate experimental designs and samples from lake trout, brown trout and Atlantic salmon, we evaluated whether freezing OF alters the way in which it modifies sperm swimming. Sperm had improved swimming performance in the presence of OF over plain water, and the effect did not depend on whether the OF had previously been frozen. Freezing OF does not seem to alter the way it influences sperm. This allows the researcher to create a ‘spice rack’ of OF samples that can be used in studies on cryptic female choice, and opens up the possibility to compare animals mating under large spatial and temporal variability.
An annotated checklist of the fish fauna of the river systems draining the Kahuzi‐Biega National Park (Upper Congo: Eastern DR Congo)
Authors: Kisekelwa Tchalondawa , Snoeks Jos , Vreven Emmanuel 
Published: January 2020

AbstractThe Kahuzi‐Biega National Park (KBNP), situated mainly in the Eastern Highlands Ecoregion of the Upper Congo basin, is drained by the Lowa and Ulindi rivers, and some western affluents of Lake Kivu. In this study, the first list of the fish diversity of these systems is provided based on museum collections and complemented, for the Lowa River system and the western Lake Kivu affluents, with recently collected specimens (2013–2017). A total of 118 species are reported from the Lowa basin, 22 from the Ulindi basin and seven from these Lake Kivu affluents. Within the Lowa and Ulindi, respectively, five and one species, all cichlids, have been introduced. Currently, 51 species are reported from within the park, only two of which have been reported from the highlands,i.e.,Amphilius kivuensisfrom the Luha, the source of the Luka River, andClarias liocephalusfrom the headwaters of the Lake Kivu’ affluents. With a total of 30 species, Cyprinidae is by far the largest family, representing 25% of the total species diversity of the Lowa basin. It is followed by Mormyridae with 13 species (11%), Alestidae and Mochokidae with 10 species each (8%), Clariidae and Amphiliidae with eight species (7%), and Distichodontidae with six species (5%). Seven new species for science were discovered and 11 species were found to be endemic to the Lowa system. Although further exploration is needed, this underscores the importance of the KBNP in protecting the fish fauna of the Lowa basin but also highlights the park's limited coverage of the fish fauna of the Lowa basin.
Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E‐cadherin: Turbot against gilthead sea bream
Authors: Ronza Paolo , Estensoro Itziar , Bermúdez Roberto , Losada Ana Paula , Pérez‐Cordón Gregorio , Pardo Belén G. , Sitjà‐Bobadilla Ariadna , Quiroga Mª Isabel 
Published: January 2020

AbstractEnteromyxoses are relevant diseases for turbot and gilthead sea bream aquaculture. The myxozoan parasites invade the intestinal mucosa, causing a cachectic syndrome associated with intestinal barrier alteration; nonetheless, their pathological impact is different. Turbot infected by Enteromyxum scophthalmi develop more severe intestinal lesions, reaching mortality rates of 100%, whereas in E. leei‐infected gilthead sea bream, the disease progresses slowly, and mortality rates are lower. The mechanisms underlying the different pathogenesis are still unclear. We studied the distribution and expression changes of E‐cadherin, a highly conserved protein of the adherens junctions, in the intestine of both species by immunohistochemistry and quantitative PCR, using the same immunohistochemical protocol and common primers. The regular immunostaining pattern observed in control fish turned into markedly irregular in parasitized turbot, showing an intense immunoreaction at the host–parasite interface. Nevertheless, E‐cadherin gene expression was not significantly modulated in this species. On the contrary, no evident changes in the protein distribution were noticed in gilthead sea bream, whereas a significant gene downregulation occurred in advanced infection. The results contribute to the understanding of the different host–parasite interactions in enteromyxoses. Host and parasite cells appear to establish diverse relationships in these species, which could underlie the different pathological picture.
The diagnostic usefulness of the combined COMPASS 31 questionnaire and electrochemical skin conductance for diabetic cardiovascular autonomic neuropathy and diabetic polyneuropathy
Authors: D'Amato Cinzia , Greco Carla , Lombardo Giorgio , Frattina Valentina , Campo Mariagrazia , Cefalo Chiara M. A. , Izzo Valentina , Lauro Davide , Spallone Vincenza 
Published: January 2020

AbstractThe study investigated the diagnostic performance for diabetic cardiovascular autonomic neuropathy (CAN) and diabetic polyneuropathy (DPN) of the combined use of composite autonomic symptom score (COMPASS) 31, validated questionnaire for autonomic symptoms of CAN, and electrochemical skin conductance (ESC), proposed for detecting DPN and CAN. One‐hundred and two participants with diabetes (age 57 ± 14 years, duration 17 ± 13 years) completed the COMPASS 31 before assessing cardiovascular reflex tests (CARTs), neuropathic symptoms, signs, vibratory perception threshold (VPT), thermal thresholds (TT), and ESC using Sudoscan. Two patterns were evaluated: (a) the combined abnormalities in both tests (COMPASS 31+ESC), and (b) the abnormality in COMPASS 31 and/or ESC (COMPASS 31 and/or ESC). CAN (≥1 abnormal CART) and confirmed CAN (≥2 abnormal CARTs) were present in 28.1% and 12.5%, DPN (two abnormalities among symptoms, signs, VPT, and TT) in 52%, abnormal COMPASS 31 (total weighted score >16.44) in 48% and abnormal ESC (hands ESC <50 μS and/or feet ESC <70 μS) in 47.4%. Both the patterns—COMPASS 31+ESC and COMPASS 31 and/or ESC—were associated with CAN and DPN (P < .01). COMPASS 31 and ESC reached a sensitivity of 75% and 83% for confirmed CAN, and a specificity of 65% and 67% for DPN. When combining the tests, the sensitivity for CAN rose by up to 100% for CAN and the specificity up to 89% for DPN. The combination of the tests can allow a stepwise screening strategy for CAN, by suggesting CAN absence with combined normality, and prompting to CARTs with combined abnormality.