bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uptake of 137Cs in legumes plants (Vicia sativa L.) at the influence of complex bacterial preparations
Authors: Illienko V. V. , Pareniuk O.Y. , Shavanova K.E. , Nesterova N. G. , Ruban Y. V. , Shpyrka N. F. , Gudkov I. M. 
Published: November 2019

Aim. Main goal is to determine the role of individual strains of microorganisms in the accumulation of 137Cs by plants of vetch (Vicia sativa L.), under the influence of seed pre-sowing inoculation by microorganisms used in agriculture as biofertilizers and cultivated on substrate contaminated by radionuclides. Methods. Laboratory and field experiments, microbiological, gamma spectrometry. Results. The changes of morphometric indices of plants due to bacterial microflora activity are described. The modification of the radionuclide accumulation coefficient under the influence of microorganisms-inoculants has been calculated. The most effective complexes of strains of microorganisms that can reduces the accumulation of radionuclide by green mass of plants are determined. Conclusions. Using seed inoculation by bacterial strains while cultivating on radionuclide-contaminated soil was proposed as an additional measure to reduce the accumulation of radionuclides in green mass of plants.
Keywords: accumulation coefficient, Vicia sativa L., 137Cs, bacterial preparations.
Genetic patterns of breeding upon creation of the first Ukrainian waxy barley cultivar Shedevr
Authors: Kozachenko M. R. , Naumov A. G. , Vasko N. I. , Solonechnyi P. N. , Solonechna O. V. , Vazhenina O. Ye. , Zymohliad A. V. 
Published: November 2019

Aim. The study’s purpose was to establish genetic patterns of waxy barley breeding on the basis of determination of genetic and breeding characteristics of accessions with different fractional composition of starch during 10/11-year cycles of the breeding process upon creation of waxy barley cultivars. Methods. Analysis of variance, calculus of variations, correlation analysis, genetic and breeding methods were used to investigate peculiarities of genotypes in topcross, diallel and pair-crossing designs. Results. The important scientific problem concerning establishment of genetic patterns of waxy spring barley breeding was solved. We determined morpho-biological features, variability, correlations and genetic peculiarities of inheritance, heritability, genetic variation and combining ability for quantitative traits of plants as well as for amylopectin content in starch of F1 hybrids derived from forms with different fractional composition of starch. As a result of establishing the patterns, the effectiveness of creation of valuable lines and waxy barley cultivar Shedevr with amylopectin starch was demonstrated. Conclusions. The morpho-biological, breeding and genetic peculiarities of plant traits with different fractional composition of starch were determined. On this basis, the genetic patterns of waxy barley breeding were established, and the first Ukrainian waxy barley cultivar Shedevr (with amylopectin starch) was created.
Keywords: Hordeum vulgare L., accession, cultivar, amylopectin starch, genetic peculiarities.
Mutants at gliadin loci on the basis of the common wheat cultivar Bezostaya 1
Authors: Kozub N. A. , Sozinov I. A. , Bidnyk H. Ya. , Demianova N. A. , Sozinova O. I. , Karelov A. V. , Blume Ya. B. 
Published: November 2019

Aim. The aim of this study was to isolate and propagate mutants at gliadin loci developed on the basis of the common wheat cultivar Bezostaya 1. Methods. We searched for spontaneous and gamma-irradiation induced mutations at gliadin loci among the progeny of F1 and F2 plants from crosses between near-isogenic lines by gliadin loci on the basis of the cultivar Bezostaya 1, including lines with the wheat-rye 1BL.1RS translocation. To identify mutations, we performed acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins. Results. On the basis of the common wheat cultivar Bezostaya 1, five mutants (six mutations) at gliadin loci were isolated and propagated, four of which were described for the first time. Three mutations occurred at the Gli-R1 locus involved in the wheat-rye 1BL.1RS translocation (the loss of secalins, intensification of a secalin component, and increased mobility of a secalin component). Two mutations were identified in the allele Gli-B1b, one caused the null-allele at the Gli-A2 locus. Conclusions. The material of mutants is of importance for studying the role of certain groups of storage proteins and their components in quality determination, as well as mechanisms of regulation of storage protein synthesis.
Keywords: Triticum aestivum, gliadin, secalin, mutation, 1BL.1RS translocation.
Constitution of swine in ontogenesis and phylogenesis
Authors: Khokhlov A. M. , Baranovskyi D. I. , Danilovа T. M. 
Published: November 2019

Aim. Determination of the peculiarities of the formation of constitutional types in ontogenesis in animals of the modern large white breed of pigs in comparison with the wild european boar (Sus scrofa ferus) is a form of the breeding process, that has both theoretical and practical significance. Methods. The objects of the study are embryos, fetuses, newborns and adult animals of large white breed of pigs and wild boar in different periods of ontogenesis using zootechnical, morphological and mathematical-statistic methods of research. Results. Individual development of animals can be conventionally divided into two main periods: morphogenetic (or embryonic prenatal) and postmorphogenetic (or post-embryonic, postnatal). The morphogenetic period is the most crucial period in the ontogenesis of animals, the period of the highest activity of genes, which provides a fundamental placement and development of the basic functional systems of the body. Therefore, for the consideration of signs of embryonic development becomes one of the criteria of artificial selection of individuals for their natural hereditarily determined type of exteriors, constitutions, and metabolism. It particularly reflects the leading role of embryogenesis in the formation of the body structure and productive qualities of animals. Conclusions. For the species practice of selection, breeding and domestication consisted of changes in quantitative and qualitative relationships in growth and development, which, combined with subsequent targeted selection, contributed to the formation of modern breeds of pigs. The constitutional type analysis of the animals formation in ontogenesis has the most selective significance, which is primarily manifested in the exterior features. Domestication of the Sus scrofa consisted of consequently the exterior is expediently evaluated at different stages of ontogenesis, starting with the embryonic period and, especially, from birth.
Keywords: phylogeny, domestics, ontogenesis, constitution, selection, species, breed.
Genealogical analysis of patients with mental disorders
Authors: Bagatska N. V. , Glotka L. I. , Sukhov V. A. 
Published: November 2019

Aim. To determine the family accumulation of multifactorial diseases in genealogies of patients with mental disorders (MD). Methods. Genealogical analysis was carried out in the families of patients with MD by the generally accepted method. Statistical analysis of the results of research was carried out using Excel software package and SPSS Statistics 17.0. Results. The genealogical analysis of patients showed that 58.8 % of families of probands with phobic-anxiety disorders (PAD) and 74.4 % of families of patients with anxiety-depressive disorders (ADD) had genetic burden for MD. In both groups, in relatives of sick probands, psychiatric, neurological and cardiovascular diseases were significantly more often, compared to the frequency of these diseases among members of families of healthy peers, moreover in families of patients with PAD endocrine diseases were more frequent. Conclusions. The genetic burden in children and adolescents with PAD and ADD has been determined. Among the multifactorial diseases in the families of patients with PAD and ADD mental, neurological and cardiovascular diseases and unfavorable factors of environment significantly prevailed.
Keywords: genealogies, patients, mental disorders, multifactorial diseases.
The role of folate metabolism genome in the formation of triiodothyronine in children living in areas affected by the Chernobyl nuclear power plant accident
Authors: Bandazhevsky Yu. I. , Dubovaya N. F. 
Published: November 2019

Aim. Aim of this study was to determine the role of the genome of folate metabolism in the formation of triiodothyronine in children living in areas affected by the Chernobyl nuclear power plant accident. Methods. Immunochemical, mathematical and statistical. Results. The proportion of cases of elevated blood levels of triiodothyronine is statistically significantly higher in the group of children who are carriers of the G risk allele of the MTR:А2756G genetic polymorphism associated with the В12-dependent methionine synthase enzyme than in the group of children who have no this allele (MTR:2756 A/A genotype). The formation of triiodothyronine is associated with the metabolic conversion of homocysteine involving vitamin В6 as a cofactor of cystathionine β-synthase. Conclusions. The cause of thyrotoxic effects with damage to the cardiovascular system may lie in high levels of homocysteine occurring due to impaired functioning of В12-dependent methionine synthase.
Keywords: folate metabolism, triiodothyronine, genetic polymorphisms, radiation-contaminated areas.
Lactose intolerance and its association with the exogamy degree among the population of eastern Ukraine
Authors: Fedota O. M. , Babalian V. O. , Borozenets V. V. , Mazniakov S. M. , Puzik N. G. 
Published: November 2019

Aim. The aim of our study was to evaluate the relationship between exogamy degree and lactose assimialation among the population of Eastern Ukraine in the Kharkiv region. Methods. The analysis included information on consumption and assimilation of milk and dairy products by 733 people aged from 14 to 79 years were. Statistical analysis included Shapiro-Wilk test, Pearson fitting (χ2) criterion and Spearman’s correlation analysis. Results. The lactose tolerance (LT) phenotype was found in 69.5%, whereas lactose intolerance (LI) – in 8.8% (from 3.5% to 24.1% in different age groups). In the age group 30-39 years: LI frequency was 24.1% being 2.5-6 times higher than in other groups. The exogamy degree of parents (EDP) showed in the group aged from 30 to 39 years the first EDP was 15.4%, being 1.4-2.5 times less (p = 0,05) than in other groups. Among people with bone marrow disorders the proportion of people with the first and second EDP was 60.9%, being 2.1 to 6.0 times higher than in other groups. Reduced EDP or increased inbreeding level causes the similarity of the chromosomes and affects the density of contact points between them during meiosis, level of recombination can be increased and associated with multifactorial traits. Conclusions. The results can be explained by the effect of the inbreeding level reduction in the presence of a high level of exogamy of parents, which causes high genetic diversity.
Keywords: lactose intolerance, lactose tolerance, exogamy degree of parents, single nucleotide polymorphisms 13910C-T and 22018G-A, gene МСМ6.
Biomass as a factor contributing to winter wheat yield increase
Authors: Morgun V. V. , Priadkina G. A. , Stasik O. O. , Zborіvskaіa O. V. 
Published: November 2019

Aim. The search of factors influencing grain productivity, based on the comparison of the mass of dry matter in the aboveground parts of modern winter wheat varieties at the early stages of ontogenesis. Methods. Morphometric determination of biomass of the above-ground plant parts. Results. The varieties and lines of winter wheat with higher yields exceeded the less productive ones by the number of shoots per 1 m2 of soil on 8–12 % and by the dry matter weight of the above-ground plant parts on 23–34 % at the early stages of spring vegetation. According to two-year experiments, it was established a linear positive correlation (r = 0.85–0.86) of the dry matter weight of the above-ground plant parts per 1 m2 of soil during the period of stem elongation (BBCH 31-49) with the yield. Conclusions. The close relationship between yield and dry matter weight of the above-ground plant parts at the early stages of spring vegetation makes it possible to rank winter wheat varieties by potential yield. Keywords: Triticum aestivum L., grain productivity, biomass, early stages of ontogenesis.
Assessment of the grain quality indicators in introgression wheat lines by the near infrared spectroscopy method
Authors: Моtsnyi I. I. , Molodchenkova O. O. , Bezlyudnyi V. N. , Solomonov R. V. , Kulbida M. P. 
Published: November 2019

Aim. The variation of protein content and its relationship to agronomic traits of introgression wheat lines were studied to determine the differentiating ability of environment, a value of breeding traits from new sources and the relevancy of assessment methods. Methods. Growing plants under different conditions was combined with the laboratory methods for determining quality. Results. An average protein content of the lines (9.7–15.5 %) was higher in the wide-row crop and in drouth years, and the range of variation was wider in dry years in the wide-row crop, but in favorable years in the solid crop. A stable positive correlation of protein content with gluten content (0.87–0.96) and sedimentation value (0.58–0.90), and the negative correlation with yield was established. Conclusions. The parameters of plant resistance to diseases and harsh agricultural backgrounds, the determination of protein content by several methods and the differentiating ability of the environments with optimal conditions and a high agricultural background should be used for selection and objective evaluation of material. Weather conditions may limit the use of some indicators as breeding traits. The method of near-infrared spectroscopy requires constant calibrations, following measurement instructions may overestimate the protein content and affect the efficiency of the selection process.
Keywords: wheat, introgression lines, protein content, methods.
Chromosomal localization patterns of beta-galactosidase genes in streptomycetes
Authors: Polishchuk L. V. 
Published: November 2019

Aim. The aim is to identify trends of localization of genes encoding beta-galactosidase (β-gal genes) in the genomes of streptomycetes. Methods. Genetic maps of 94 strains of streptomycetes from the NCBI server database were analyzed. Results. Molecular sizes of genomes from the strains sample were from 6.84 Mbp up to 12.7 Mbp. Most of genomes (43.7 %) in the sample have molecular sizes 7.0 Mbp – 8.5 Mbp. As determined, the number of β-gal genes on genetic maps ranged from 0 to 13. 61.3 % of the strains contained 1 - 3 β-gal genes in their genomes. It has been established that these genes are located in the genomes of streptomycetes, as a rule, in terminal regions. 79 % of the studied β-gal genes had a molecular size from 1.5 kb. up to 2.1 kb. Conclusions. The following patterns of localization of β-gal genes in the genomes of streptomycetes were established: 1) Tendency of localization of the overwhelming majority of β-gal genes in the terminal regions of streptomycete genomes; 2) Trend has been established for the localization in the core region of individual β-gal genes, both in genomes of large molecular size and containing more than 8 genes; 3) The streptomycetes genomes contain the β-gal genes mainly of the size from 1.5 kb up to 2.1 kb regardless of molecular sizes of their genomes and the number of β-gal genes in them.
Keywords: β-gal gene, genome, Streptomyces, molecular size.
Assessment of the potential of Artemisia annua L. as a source of biologically active substances in Belarus
Authors: Shutava H. G. , Shysh S. N. , Shabunya P. S. , Fatykhava S. A. , Skakovski E. D. , Tychinskaya L. Yu. 
Published: November 2019

Aim. The aim of the work was to assess the composition of biologically active compounds in plants of Artemisia annua L. Methods. The plants were grown on the experimental plot of the Central Botanical Garden of the National Academy of Sciences of Belarus. Essential oil from the above-ground mass was isolated by the method of water-steam distillation. To analyze the content of flavonoids and hydroxycinnamic acids, we used the method of quantitative extraction and spectrophotometric determination of the total content of hydroxycinnamic acids in the presence of flavonoids in plant extractive substances, and the method of Folin-Chocalteu was used to determine the content of phenolic compounds. Analysis of the essential oils was performed by GC and NMR. The analysis of the content of phenolic acids in extracts was performed by HPLC. Results. The genetic potential of A. annua was evaluated under conditions of the central agroclimatic zone of Belarus. The yield and composition of essential oils, the content of phenolic compounds and flavonoids, the composition of phenolic acids were studied. Conclusions. A. annua in the conditions of the central agroclimatic zone of Belarus contains essential oil, which is dominated by isoartemisia ketone, β-selinene, β-myrcene and camphor. In the above-ground mass phenolic acids including chlorogenic acid and isomers of caffeoylquinic acid were predominant in the composition of phenolic compounds.
Keywords: annual wormwood, essential oil, phenolic compounds, phenolic acids.
Antiradiation properties of extracts from Hypericum perforatum L.
Authors: Shamilov E. N. , Abdullaev A. S. , Shamilli V. E. , Azizov I. V. 
Published: November 2019

Aim. Considering the high biological activity of the components of the St. John’s wort, we conducted studies to identify its radioprotective effect. Methods. Studies were conducted on adult Vistar rats with an average weight of 100-120 grams.3 hours before irradiation, the extract from St. John’s wort was intraperitoneally administered to animals at a concentration of 50 mg/kg. Irradiation was carried out once with doses of 5 and 7 Gy on the “Rhund-20000” facility with an average radiation dose rate MD = 0.3069 Gr/s. The frequency of chromosomal abnormalities was recorded on the mitotic cells of the bone marrow. Results. Acute irradiation with a dose of 5 Gy led to cell division inhibition. The number of chromosomal aberrations increased due to structural disorders (deletions, fragments, inversions) up to 6.8%.In animals that received a dose of 7 Gy after a single irradiation, the frequency of chromosomal abnormalities increased to 11.02%. The introduction of the Hypericum perforatum L. extract softened the effect of ionizing radiation, reducing the mutational effect of radiation. It not only contributed to the restoration of the cell division, but also reduced the proportion of structural disorders. Conclusions. Hypericum extract can be considered to be promising as an anti-radiation agent of plant origin.
Keywords: rats, chromosomal abnormalities, marrow, irradiation, extract.
Bioinformatic identification of Ty1/Copia-like transposable elements in Deschampsia antarctica E. Desv.
Authors: Andreev I. O. , Konvalyuk I. I. , Kunakh V. A. 
Published: November 2019

Aim. Identification of Ty1/Copia-like transposable elements in Deschampsia antarctica E Desv. in silico. Methods. Bioinformatic analysis of sequence read archives of D. antarctica genome and transcriptome contained in the GenBank database was conducted. The search was carried out using the rice Ty1/Copia TE Tos 17 as a reference sequence. Results. The search revealed the sequences of Ty1/Copia retrotransposons in the D. antarctica genome to show a high level of identity (up to 75% in the homologous regions) to Tos 17. The uneven distribution of the found reads along the reference sequence indicates the existence of a group of the sequences in the genome to contain elements typical of the family and have varying degrees of identity to Tos 17, with more conservative ones being represented in correspondingly greater numbers among the found reads. The presence of the reads identical to Tos 17 in transcriptome indicates that the TE sequences identified in genome have a certain background level of transcriptional activity. Conclusions. Transcriptionally active Ty1/Copia-like transposable elements were identified in silico in D. antarctica genome using methods of bioinformatics. The sequences found can be used to construct primers for PCR and to analyze the activity of the TE of this family in D. antarctica in further studies.
Keywords: bioinformatic analysis, Deschampsia antarctica E Desv., transposable elements, Ty1/Copia.
Conservation of dinitroani-line/phosphorothioamidate site of α-tubulin in Plasmodium species distributed in India
Authors: Karpov P. A. , Demchuk O. M. , Ozheredov S. P. , Spivak S. I. , Yemets A. I. , Blume Ya. B. 
Published: November 2019

Aim. To evaluate polymorphism of dinitroaniline/phosphorothioamidate binding site in α-tubulin molecules from Plasmodium species (P. falciparum, P. vivax, P. ovale, and P. malariae) and strains discovered in India. Methods. Literature and database search. Bioinformatical comparison of protein sequences and structures. Multiple sequence alignment, phylogenetic profiling, protein structure modeling, etc. Results. 14 complete sequences of α-tubulin from four Plasmodium species were selected from UniProtKB. Despite certain differences in complete sequences of α-tubulin molecules from different Plasmodium species and strains, sites of their interaction with dinitroaniline and phosphorothioamidate compounds were shown to be identical. Conclusions. Complete identity of dinitroaniline/phosphorothioamidate binding sites in all studied isotypes of α-tubulin molecules from P. falciparum, P. vivax, P. ovale, and P. malariae was confirmed. This suggests identity of the ligand-protein interaction mechanism and similar destabilizing effect of dinitroaniline and phosphorothioamidate compounds on microtubules of all Plasmodium species officially registered in India.
Keywords: malaria, Plasmodium, α-tubulin, dinitroanilines, phosphorothioamidates, binding site.
Identification of plant α-tubulin amino acids playing a key role in specific binding of nitroaniline compounds
Authors: Ozheredov S. P. , Demchuk O. M. , Karpov P. A. , Spivak S. I. , Blume Ya. B. 
Published: November 2019

Aim. Computational prediction of amino acid residues critical for specific binding of nitro- and dinitroaniline compounds in plant α-tubulin. Methods. Protein structure modeling (I-Tasser, Grid-computing) and ligand library preparation, molecular docking (CCDC Gold), molecular dynamics (MD, Gromacs computing in Grid). Evaluation of the amino acid ensemble associated with ligand binding based on results of MD energy perturbations of protein-ligand complex. Results. The structural model of plant α-tubulin from Avena sativa was build. Also, the virtual library of 25 nitroaniline compounds was prepared. The docking of ligands into the interdimer contact of α-tubulin and MD simulations of the leading complexes reveal differences in ligands conformational energy during the exchange between free and binding states. The mean number of hydrogen bonds and dynamics of their formation in complex were compared. These computations allow us to select a.a. residues playing key role in specific interaction with nitro- and dinitroaniline compounds in plant α-tubulin. Conclusions. Computational prediction specify 28 a.a. residues playing the main role in binding of nitro- and dinitroaniline compounds with plant α-tubulin from Avena sativa: Arg2, Glu3, Ile4, Cys129, Thr130, Gly131, Leu132, Gln133, Gly134, Gly162, Lys163, Lys164, Ser165, Leu242, Arg243, Asp245, Gly246, Ala247, Ile248, Asn249, Val250, Asp251,Val252, Thr253, Glu254, Phe255, Thr257, Asn258.
Keywords: plant, α-tubulin, nitroaniline compounds, molecular docking, molecular dynamics, ligand binding.