bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surgical Treatment for Anomalous Origin of the Right Coronary Artery from the Pulmonary Artery: A Case Report with Five-Year Follow-up
Authors: Teng Peng , Li Weidong , Ni Yiming 
Published: October 2020

Abstract
Background Anomalous origin of the right coronary artery from the pulmonary artery (ARCAPA) is a rare congenital heart disease affecting about 0.002% of the population. Knowledge of ARCAPA is almost collected from case reports. The aim of this study was to provide a rare case to better understand this rare congenital coronary anomaly. Case presentation We report a rare case of an 18-year-old male who was initially referred because of heart murmur. Dilated and tortuous coronary arteries were detected by echocardiography and congenital coronary anomaly was suggested. Further coronary CT angiography confirmed the diagnosis of ARCAPA. Although dual coronary system provides favorable long-term outcome, bypass surgery was considered technically difficult due to the huge mismatch of caliber between the right coronary artery and graft vessels. Eventually, simple right coronary artery ligation was performed. The patient was followed up for about 5 years without evidence of atherosclerosis or myocardial ischemia.Conclusions ARCAPA presents as a rare congenital heart disease with variable clinical manifestations. Surgical treatment is highly recommended to re-establish dual coronary system and prevent further complications. To our best knowledge, only about 200 cases of ARCAPA has been reported.
Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)
Authors: Cao Yi-Fan , Chen Hui-Xia , Li Yang , Zhou Dang-Wei , Chen Shi-Long , Li Liang 
Published: October 2020

Abstract
Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites from the Tibetan antelope remains limited. Methods: Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences [i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1)] were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes.Results: Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum sp. n. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions: A new species of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum sp. n. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.
Recovery of preoperative absolute knee extension and flexion strength after ACL reconstruction
Authors: Piussi Ramana , Broman Daniel , Musslinder Erik , Beischer Susanne , Thomée Roland , Senorski Eric Hamrin 
Published: October 2020

Abstract
BackgroundThe recovery of muscle function after an Anterior Cruciate Ligament (ACL) reconstruction is most commonly reported as limb-to-limb differences using the Limb Symmetry Index (LSI), which is not free from limitations. The purpose of this study was to compare the proportion of patients who recover their Preoperative Absolute Muscle Strength (PAMS) 8 and 12 months after ACL reconstruction with the proportion of patients who recover their symmetrical knee strength. A secondary aim was to assess the relationship between psychological Patient-Reported Outcomes (PROs) and recovering PAMS at 8 and 12 months after ACL reconstruction and rehabilitation.MethodPreoperative, 8- and 12-month results from quadriceps and hamstring strength tests and PROs for 117 patients were extracted from a rehabilitation registry. Individual preoperative peak torques from strength tests were compared with results from the 8- and 12-month follow-ups respectively. Patients were defined as having recovered their PAMS upon reaching 90% of their preoperative peak torque for both quadriceps and hamstring strength. Patients were defined as having recovered their LSI upon reaching a value ≥ 90% when comparing the results for their injured knee with those of their healthy knee. Correlations between the recovery of PAMS and PROs at 8 and 12 months were analyzed.ResultsThere was no difference in the proportion of patients who recovered their PAMS compared with patients who recovered their LSI. In all, 30% and 32% of the patients who recovered their LSI had not recovered their PAMS at 8 months and 12 months respectively. In the patients who had recovered their PAMS, 24% and 31% had not recovered their symmetrical LSI at 8 months and 12 months respectively. There was no significant correlation between the recovery of PAMS and psychological PROs. ConclusionThe use of both PAMS and LSI provides more detailed information on the recovery of muscle strength after ACL reconstruction. The recovery of PAMS was not correlated with psychological traits, which implies that both PROs and PAMS are important when evaluating patients after ACL reconstruction.Trial RegistrationEthical approval has been obtained from the Regional Ethical Review Board in Gothenburg, Sweden (registration numbers: 265-13, T023-17).
Changes in Serum Homocysteine Concentrations and their Prognostic Value in Pregnant Chinese Women with Preeclampsia : A Retrospective Study
Authors: Qin Wanyuan , Hu Xuehua , Fu Chunyun , Tang Yuqun , Lu Xiangjun , Deng Zuoxin , Huang Qiang , Wei Xiaofen , Chen Yan , Wang Junlong , Huang Kangkai , Jing Junpeng 
Published: October 2020

Abstract
Background: This study evaluated whether changes in homocysteine concentrations in pregnant women with preeclampsia might be useful for predicting pregnancy-related outcomes.Methods: This retrospective study evaluated 2,745 pregnant Chinese women (1,368 women with preeclampsia and 1377 healthy pregnant controls) at two Chinese centres. Medical records were reviewed to collect data regarding maternal age, homocysteine concentrations, gestational weight gain, body mass index (BMI), neonatal weight, Apgar score, previous abortions, previous deliveries, adverse pregnancy history, and pregnancy-related outcomes.Results: Maternal serum homocysteine concentrations were significantly higher in the preeclampsia group (vs. the control group) during the second trimester (P<0.0001) and the third trimester (P<0.0001). Foetal death was also significantly more common in the preeclampsia group. Significant differences (P<0.05) were also observed between the preeclampsia and control groups in terms of maternal age, gestational weight gain, BMI, neonatal weight, Apgar score, previous abortions, previous deliveries, gestation length, adverse pregnancy history, and pregnancy outcomes. Women with preeclampsia had elevated homocysteine concentrations that increased from the first trimester until the third trimester. Among women with preeclampsia, the risk of foetal death was significantly associated with maternal age, BMI, neonatal weight, Apgar score, and serum homocysteine concentrations during the second trimester (P=0.022) and the third trimester (P=0.034). Conclusion: Our results indicate that foetal death in women with preeclampsia was associated with maternal age, BMI, neonatal weight, and Apgar score. Furthermore, foetal death was associated with high maternal homocysteine concentrations during the second and third trimesters.
TNFAIP3&nbsp;Suppresses Lung Cancer Progression via Regulating Cell Proliferation and Apoptosis
Authors: Chen Han , Gao Jie , Yu Yongsheng , Zhou Qian , yongqi Shan 
Published: October 2020

Abstract
Background: The ubiquitin-editing enzyme TNF inducible protein 3 (TNFAIP3) is a crucial regulator of inflammation and immunity. It is also involved in tumorigenesis of various cancers such as lymphomas, colorectal tumors and breast cancer. In this study, we aimed to explore the role and regulatory mechanism of TNFAIP3 in lung cancer. Methods: The expression of TNFAIP3 was determined in the Cancer Genome Atlas (TCGA) database. The levels of TNFAIP3 in lung cancer tissues was determined by immunohistochemistry (IHC) assay. TNFAIP3 knockdown and overexpression were performed, followed by further evaluation of cell viability, cell cycle and apoptosis. Cell cycle and apoptosis were observed by using flow cytometry and the key regulatory proteins were detected by western blotting. Colony formation assessment and EdU assay were adopted to check cell proliferation. Results: TNFAIP3 expression was downregulated in lung cancer tissues at both mRNA and protein levels, comparing with that in adjacent non-tumor tissues. Consequently, the colony formation ability of lung cancer cells was enhanced, and the number of EdU positive lung cancer cells was increased. By contrast, elevated TNFAIP3 expression resulted in decreased colony formation ability of lung cancer cells. Mechanistically, TNFAIP3 overexpression rendered cell cycle of lung cancer cells halted at G0/G1 phase and caused apoptosis of lung cancer cells.Conclusion: Our data suggested that TNFAIP3 exhibits tumor suppressive roles in lung cancer.
Metabolome and Transcriptome Analysis of Flavor Components and Flavonoid Biosynthesis in Fig Fruit (Ficus Carica L.) After Bagging
Authors: Wang Ziran , Song Miaoyu , Wang Zhe , Chen Shangwu , Ma Huiqin 
Published: October 2020

Abstract
Background: Bagging can improve the appearance of fruits and increase the food safety and commodification, it also has effects on intrinsic quality of the fruits, which was commonly reported negative changes. Fig can be regarded as a new model fruit with its relatively small genome size and long fruit season. Results: In this study, widely targeted metabolomics based on HPLC MS/MS and RNA-seq of the fruit tissue of the ‘Zibao’ fig before and after bagging were analyzed to reveal the metabolites changes of the edible part of figs and the underneath gene expression network changes. A total of 771 metabolites were identified in the metabolome analysis using fig female flower tissue. Of these, 88 metabolites (including one carbohydrate, eight organic acids, seven amino acids, and two vitamins) showed significant differences in fruit tissue before and after bagging. Changes in 16 structural genes, 13 MYB transcription factors, and endogenous hormone (ABA, IAA, and GA) metabolism and signal transduction-related genes in the biosynthesis pathway of flavonoids after bagging were analyzed by transcriptome analysis. KEGG enrichment analysis also determined significant differences in flavonoid biosynthesis pathways in female flower tissue before and after bagging. Conclusions: This work provided comprehensive information on the composition and abundance of metabolites in the female flower tissue of fig. The results showed that the differences in flavor components of the fruit before and after bagging could be explained by changes in the composition and abundance of carbohydrates, organic acids, amino acids, and phenolic compounds. This study provides new insights into the effects of bagging on changes in the intrinsic and appearance quality of fruit.
Exploring the Mechanisms Underlying the Therapeutic Effect of Salvia Miltiorrhiza Against Diabetic Nephropathy Using Network Pharmacology and Molecular Docking
Authors: Zhang Li-Li , Han Lin , Wang Xin-Miao , Wei Yu , Zheng Jing-Hui , Zhao Lin-Hua , Tong Xiaolin 
Published: October 2020

Abstract
BackgroundThe mechanisms underlying the therapeutic effect of Salvia Miltiorrhiza (SM) against diabetic nephropathy (DN) using systematic network pharmacology and molecular docking methods were examined.MethodsTCMSP database was used to screen the active ingredients of SM. Gene targets were obtained using Swiss Target Prediction and TCMSP databases. Related targets of DN were retrieved from the Genecards and DisGeNET databases. Next, a PPI network was constructed using the common targets of SM-DN in the STRING database. The Metascape platform was used for GO function analysis and Cytoscape plug-in ClueGO was used for KEGG pathway enrichment analysis. Molecular docking was performed using iGEMDOCK and AutoDock Vina software. Pymol and LigPlos were used for mapping the network. ResultsSixty-six active ingredients and 189 targets were screened from SM. Among them, 64 targets overlapped with DN targets. The PPI network diagram revealed that AKT1, VEGFA, IL6, TNF, MAPK1, TP53, EGFR, STAT3, MAPK14, and JUN were the top 10 relevant targets. GO and KEGG analyses mainly focused on advanced glycation end products, oxidative stress, inflammatory response, and immune regulation. Molecular docking revealed that the potential target genes closely related to DN, including TNF, NOS2, and AKT1, were more stable in combination with salvianolic acid B, and their stability was better than that of tanshinone IIA.ConclusionThis study reveals the active components and potential molecular mechanisms involved in the therapeutic effect of SM against DN and provides a reference for the wide application of SM in clinically managing DN.
The Mechanism of Aidi Injection on Breast Cancer Based on Network Pharmacologyanalysis
Authors: Su Jing , Liu Kedi , Tao Xingru , Li Fei , Zhao Shi , Bai Yang , Lu Xinming , Li Jing , Chen Sha , Duan Jialin , Wei Peifeng , xi Miaomiao 
Published: October 2020

Abstract
Background: Aidi injection (ADI)is a Chinese patent medicine with anti-cancer effect, which has been used to treat breast cancer (BC) in China for many years, but its potential pharmacological mechanism is still indeterminacy. In this resaearch, network pharmacology, a systematic and comprehensive approach, was used to reveal ADI's potential pharmacological mechanism in treating BC for the first time.Methods: Databases were used to collect targets related to the bioactive components of ADI and BC. the relevant networks were established by the string database, and were screened potential bioactive components and core targets. Eventually, core targets and pathway enrichment were analyzed by DAVID database.Results: As the results showed, we collected 99 bioactive ingredients,345 ADI-related targets after deduplication and 368 BC-related targets. Of these, 108 common targets were overlapped. We then performed an enrichment analysis on the common target network and the protein-protein interaction (PPI) network.Conclusion: The results showed that ADI may inhibit breast cancer through seven important signal pathways involved in the "regulation of vascular endothelial function", "inflammatory response" and "apoptosis” biological processes. Through further clustering and enrichment analysis of the PPI network of ADI’s bioactive component targets and BC-related targets, we found that cancer, ErbB, MAPK, TLR, chemokine, p53 and cell cycle signaling pathway, mainly contributed to the effects of ADI in treating BC. In conclusion, this study reveals the possible mechanism of ADI in treating BC, and provides a new direction for drug development for ADI in treating BC.
Long noncoding RNA Gm44593 attenuates oxidative stress from age-related hearing loss by regulating miR-29b/WNK1
Authors: Li Qian , Zang Yanzi , Sun Zhanwei , Zhang Wenqi , Liu Hongjian 
Published: October 2020

Abstract
AmisLong noncoding RNA has been reported to play important role in various disease. However, the function of lncRNA in age-related hearing loss still unclear. The aim of our study is to investigate the function and mechanism of lncRNA Gm44593 in AHLMaterials and methodsATP content, JC-1 assay, mitochondrial content, cell death rates and dual-luciferase reporter assay were performed to assess the function of lncRNA Gm44593 in HEI-OC1 cells and the mouse cochlea.ResultsThe expression of lncRNA Gm44593 was significantly upregulated upon H2O2 and starvation treatment. Overexpression of lncRNA Gm44593 manifestly reduced the cell death rates. The ATP content, mtDNA content and mitochondrial membrane potential were alleviated upon overexpression of lncRNA Gm44593. We also proved that miR-29b is the direct target of lncRNA Gm44593. Overexpression of miR-29b completely restored the effect induced by lncRNA Gm44593. In addition, we provided evidences that WNK1 is the direct target of miR-29b.Conclusionour research uncovers the role of lncRNA Gm44593 in age-related hearing loss. We provide new insights into potential therapeutic targets for the amelioration of age-related hearing loss.
SSACBKM: An Integration Model for Biomedical Relationship Extraction
Authors: Shi Huiping , Zhou Xiaobing 
Published: October 2020

Abstract
Background: The cause of the disease is one of the main contents of biomedical research. Extracting effective relational information from a large number of biomedical texts has important applications for biomedical research. At present, most of the work of biomedicine is to use manual screening or use rule-based or feature-based pipeline network models to obtain screening characteristics. These methods require a lot of time to design specific rules or features to complete specific tasks, resulting in some features that non-compliant features cannot be filtered out.Results: The model gets micro-F1 scores of 0.802 and 0.876 on the Chemprot data set and DDI data set, respectively. The resources that can be used in this project can be found in https://github.com/HunterHeidy/DDICPI-.Conclusions: Experiments have proved that without Bert, you can get good results by learning from Bert<s core ideas.
Atypical Resting-State Functional Connectivity of Intra/Inter-Sensory Networks is Related to Symptom Severity in Young Boys with Autism Spectrum Disorder
Authors: Wang Jia , Wang Xiaomin , Wang Runshi , Duan Xujun , Chen Heng , He Changchun , Zhai Jinhe , Chen Huafu , Wu Lijie 
Published: October 2020

Abstract
Background Autism spectrum disorder (ASD), a neurodevelopmental disorder, has been reported with an altered brain connectivity pattern in the sensory network using resting-state functional magnetic imaging (rs-fMRI) compared to typical developing participants (TDs). However, there is still no consistent conclusion. In the current study, we investigated the alterations of the intra-network and inter-network connectivity pattern relating to sensory in children with ASD compared with TDs, and further assessed whether these alterations are associated with autistic behavioral symptoms. Methods rs-fMRI was used to assess young boys with ASD (N=29) and TD (N=29), aged 3-7-years. Groups were matched for age and handiness. Spatial patterns of the sensory rest state networks (RSNs) were obtained using group Independent component analysis (ICA) method, and between-groups differences were evaluated within each sensory network. Then, the time series of each RSN were extracted from each participant preprocessed data. Correlation analysis was assessed among intra- and inter-network functional connectivity (FC) and symptom severity in children with ASD. Results Four sensory components were identified, including auditory network (AN), higher visual network (HVN), primary visual network (PVN) and sensorimotor network (SMN). Functional images revealed two sensory networks exhibiting significant increased FCs in ASD group, located within AN and SMN. Higher positive connectivity between PVN and HVN in ASD group is associated with symptom severity. Conclusion Current study might shed light that the abnormal connectivity patterns of sensory network regions may underlie impaired higher-order multisensory integration in ASD children, and social impairment of ASD are caused probably by aberrant FCs involving inter/intra- sensory network.
Botulinum Toxin Injections as an Effective Treatment for Patients with Hailey-Hailey or Darier Disease: An open-label 6-month pilot interventional study
Authors: Dreyfus Isabelle , Maza Aude , Rodriguez Lauriane , Merlos Margot , Texier Hélène , Rousseau Vanessa , Sommet Agnès , Mazereeuw-Hautier Juliette 
Published: October 2020

Abstract
Background: Patients with Hailey-Hailey and Darier diseases present with disabling inflammatory lesions located in large skin folds, which are often exacerbated or induced by sweating. Quality of life is highly impaired because of pain and recurrent skin infections. An improvement in skin lesions after botulinum toxin A injections has previously been reported in some patients but no prospective interventional studies are available. The aim of this open-label, 6-month, interventional pilot study (NCT02782702) was to evaluate the effectiveness and safety of botulinum toxin A injections. Thirty patients with moderate to very severe skin lesions located in folds were enrolled in the study. Results: Thirty patients (26 Hailey-Hailey / 4 Darier) were included. Botulinum toxin A proved effective within the first month in two-thirds of patients, taking all study parameters (itchiness, cutaneous pain, sweating and odour, infections, psychosocial impairment and quality of life) into account and persisted during the 6-month follow-up period. No patient was classed as a BtxA non-responder, but 11 (37%) patients, who were the most severe ones, experienced a relapse during the study. No serious side effects were reported. Mild transient clear fluid discharge at the site of the injections was reported for 27% of patients. Conclusions: Botulinic toxin is an effective and safe treatment for Hailey-Hailey and Darier diseases. Further studies should evaluate the benefit of increased doses or combined treatments for the most severe patients.Timing of approvals:- 25, February 2015: approval by the institutional ethics committee- 2, June 2015: registration under the European Eudract identifier 2015-000379-29 (https://eudract.ema.europa.eu/index.html)- 31, July 2015: approval by the French national health authority, ANSM - 31, August 2015: implementation agreement signed by the sponsor. - 25, May 2016: registration under the ClinicalTrials.gov identifier NCT02782702 (https://clinicaltrials.gov/ct2/show/NCT02782702?cond=NCT02782702&draw=2&rank=1).