bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome wide association analysis on semen volume and milk yield using different strategies of imputation to whole genome sequence in French dairy goats
Authors: Talouarn Estelle , Bardou Philippe , Palhière Isabelle , Oget Claire , Clément Virginie , Tosser-Klopp Gwenola , Rupp Rachel , Robert-Granié Christèle 
Published: January 2020

Abstract

Background:Goats were domesticated 10,500 years ago to supply humans with useful resources. Since then, specialized breeds that are adapted to their local environment have been developed and display specific genetic profiles. The VarGoats project is a 1,000 genomes resequencing program designed to cover the genetic diversity of theCapragenus. In this study, our main objective was to assess the use of sequence data to detect genomic regions associated with traits of interest in French Alpine and Saanen breeds.Results:Direct imputation from the GoatSNP50 BeadChip genotypes to sequence level was investigated in these breeds using FImpute and different reference panels: within-breed, allCapra hircussequenced individuals, European goats and French mainland goats. The best results were obtained with the French goat panel with allele and genotype concordance rates reaching 0.86 and 0.75 in the Alpine and 0.86 and 0.73 in the Saanen breed respectively. Mean correlations tended to be low in both breeds due to the high proportion of variants with low frequencies.For association analysis, imputation was performed using FImpute for 1,129 French Alpine and Saanen males using within-breed and French panels on 23,338,436 filtered variants. The association results of both imputation scenarios were then compared. In Saanen goats, a large region on chromosome 19 was significantly linked to semen volume and milk yield in both scenarios. Significant variants for milk yield were annotated for 91 genes on chromosome 19 in Saanen goats. For semen volume, the annotated genes include YBOX2 which is related to azoospermia or oligospermia in other species. New signals for milk yield were detected on chromosome 2 in Alpine goats and on chromosome 5 in Saanen goats when using a multi-breed panel.Conclusion:Even with very small reference populations, an acceptable imputation quality can be achieved in French dairy goats. GWAS on imputed sequences confirmed the existence of QTLs and identified new regions of interest in dairy goats. Adding identified candidates to a genotyping array and sequencing more individuals might corroborate the involvement of identified regions while removing potential imputation errors.
Spouse’s participation in perinatal care: a qualitative study
Authors: mehran nahid , Hajian Sepideh , simbar Masoumeh , majd hamid alavi 
Published: January 2020

Abstract
Background: Pregnancy is one of the most important and sensitive periods for any woman that in the case of the support of her relatives, especially her spouse, feels more ability to tolerate it’s difficulties and gives them good memory. However, there are very few studies about the participation of spouses/partners in perinatal period in Iran. Therefore, the present study aimed to explain the concept of spouses' participation in perinatal care.
Methods: This is a qualitative study that was carried out in Qom, Iran in 2018. Purposive sampling was done from pregnant or postpartum women, spouses, midwifery care providers and key informants according to study inclusion criteria. Semi-structured in-depth interviews were carried out until data saturation. Data analysis was done based on conventional content analysis approach according to Graneheim & Lundman steps and using MaxQDA software (v.10). To ensure the trustworthiness of data, the five Guba and Lincoln criteria were used. Also, COREQ checklist was used for reporting this qualitative research.
Results: fifty-three final codes were classified into 18 sub-categories, 7 main categories and 3 themes include of empathy (emotional understanding, cognitive understanding), accountability (supporting, position management, compassion) and consequences (help improve family function, improve maternal-neonatal health).
Conclusion: since, spouses’ participatory behaviors in perinatal period can lead to improve maternal and neonatal health and family function, it is necessary to be considered in family-based health promotion policies and programming based on these policies such as improving the public culture of spouse participation in the process of child-rearing.
Key words: spouse, partner, participation, perinatal care
Targeting of immunosuppressive myeloid cells from glioblastoma patients by modulation of size and surface charge of lipid nanocapsules
Authors: Pinton Laura , Magri Sara , Masetto Elena , Vettore Marina , Schibuola Ilaria , Ingangi Vincenzo , Marigo Ilaria , Matha Kevin , Benoit Jean-Pierre , Puppa Alessandro Della , Bronte Vincenzo , Lollo Giovanna , Mandruzzato Susanna 
Published: January 2020

Abstract
Background: Myeloid Derived Suppressor Cells (MDSCs) and Tumor-Associated Macrophages (TAMs) are two of the major players involved in the inhibition of anti-tumor immune response in cancer patients, leading to poor prognosis. Selective targeting of myeloid cells has therefore become an attractive therapeutic strategy to relieve immunosuppression and, in this frame, we previously demonstrated that lipid nanocapsules (LNCs) loaded with lauroyl-modified gemcitabine efficiently target monocytic MDSCs in melanoma patients. In this study, we investigated the impact of the physico-chemical characteristics of LNCs, namely size and surface potential, towards immunosuppressive cell targeting. We exploited myeloid cells isolated from glioblastoma patients, which play a relevant role in the immunosuppression, to demonstrate that tailored nanosystems can target not only tumor cells but also tumor-promoting cells, thus constituting an efficient system that could be used to inhibit their function.
Results: The incorporation of different LNC formulations with a size of 100 nm, carrying overall positive, neutral or negative charge, was evaluated on leukocytes and tumor-infiltrating cells freshly isolated from glioblastoma patients. We observed that the maximum LNC uptake was obtained in monocytes with neutral 100 nm LNCs, while positively charged 100 nm LNCs were more effective on macrophages and tumor cells, maintaining at low level the incorporation by T cells. The mechanism of uptake was elucidated, demonstrating that LNCs are incorporated mainly by caveolae-mediated endocytosis.
Conclusions: We demonstrated that LNCs can be directed towards immunosuppressive cells by simply modulating their size and charge and provided a novel approach to exploit nanosystems for anticancer treatment in the frame of immunotherapy.
Characterization of Cystoisospora suis sexual stages in vitro
Authors: Feix Anna Sophia , Cruz-Bustos Teresa , Ruttkowski Bärbel , Joachim Anja 
Published: January 2020

Abstract
Background:
The porcine coccidium Cystoisospora suis is characterized by a complex life cycle during which asexual multiplication is followed by sexual development with two morphologically distinct cell types, the micro- and macrogametes. Genes related to the sexual stages and cell cycle progression were previously identified in related Apicomplexa. Here, the sexual stages of C. suis were characterized in vitro morphologically and for temporal expression changes of the mentioned genes to gain insight into this poorly known phase of coccidian development.
Methods:
Sexual stages of C. suis (micro- and macrogametes) developing in vitro in porcine intestinal epithelial cells were examined by light and electron microscopy. The transcriptional levels of genes related to merozoite multiplication and sexual development were evaluated by quantitative real-time PCR at different time points of cultivation. Dynein light chain and male gamete fusion factor HAP2 expression is presumably restricted to microgametes. Tyrosin-rich proteins are a part of the wall-forming bodies and oocyst wall proteins. The RAD51/Dmc1 protein plays a role during the cell division process, and Nima-related protein kinases regulate the cell cycle and are associated with male or female Plasmodium gametocytes. Transcription levels were compared for parasites in culture supernatants at 6–9 doc (day of cultivation) and intracellular parasites at 6–15 doc.
Results:
Sexual stage of C. suis could be found during 8–11 doc in vitro . Microgametes had a spherical body (3.0–5.0 µm) and two flagella (10.8–12.3 µm). Macrogametes were spherical with a diameter of 11.5–13.0 µm. Merozoite gene transcription peaked on 10 doc and then declined. Genes related to the sexual stages and cell cycle showed an upregulation with a peak on 13 doc, after which they declined.
Conclusions:
The present work linked gene expression changes to the detailed morphological description of C. suis sexual development in vitro , including fertilization, meiosis and oocyst formation in vitro . Following this process at the cellular and molecular level will elucidate details on potential bottlenecks of development of C. suis (and coccidian parasites in general) which could be exploited as novel targets for control.
Echocardiographic signs of subclinical cardiac function impairment in Duchenne dystrophy gene carriers
Authors: Kincl Vladimír , Panovský Roman , Pešl Martin , Máchal Jan , Juříková Lenka , Haberlová Jana , Masárová Lucia 
Published: January 2020

Abstract
Aim to assess subclinical cardiac function impairment in Duchenne dystrophy (DMD) female carriers Methods Forty-four female subjects proved as DMD carriers underwent echocardiographic examination including tissue Doppler imaging (TDI) of mitral and tricuspid annulus. Seventeen age-matched healthy female subjects served as controls. Results A significant differences in peak systolic annular velocity (Sa) between carriers and controls were found for lateral and septal part of the mitral annulus and for tricuspid annulus (0.09 vs 0.11 m/s, p<0.001, 0.08 vs 0.09 m/s, p<0.01 and 0.13 vs 0.14 m/s, p=0.02 respectively). There was also difference in early diastolic velocity (Ea) of the septal part of the mitral annulus (0.11 vs 0.13 m/s, p=0.03). Conclusion The subclinical deterioration of systolic function is presented even in asymptomatic DMD female carriers.
The efficacy of Enhanced recovery after surgery (ERAS) for elderly patients with intertrochanteric fractures received surgery: study protocol for a randomized, blinded, controlled trial
Authors: Yin Mengchen , Yan Yinjie , Fan Zhaoxiang , Fang Niankang , Wan Hongbo , Mo Wen , Wu Xuequn 
Published: January 2020

Abstract
Background: Intertrochanteric fracture (ITF) is increasing with the rapid increase in the aging population, often causes a high mortality rate in old patients and increases the economic burden of the family and society. ERAS (Enhanced Recovery after Surgery) is a powerful guarantee for patients to accelerate their recovery after surgery. TCM (traditional Chinese medicine) promote repair of injured tissues and eliminate traumatic aseptic inflammation. Therefore, this prospective randomized controlled clinical trial aims to evaluate the clinical effect of the evidence-based ERAS pathway of integrating TCM with and western medicine on perioperative outcomes in ITF patients undergoing intramedullary fixation, and provide reliable evidence-based data for applying the program to clinical practice.
Methods/design: We will conduct a prospective randomized, blinded, controlled trial to compare the effectiveness of ERAS care pathway with traditional care pathway, and to investigate whether the ERAS care pathway can improve the perioperative outcome in ITF patients undergoing intramedullary fixation. A total of 60 patients with ITF will be enrolled and treated with the two care pathway, respectively. Length of stay, economic indicators, Harris score, VAS score, time get out of bed, 30-day readmission rates, postoperative transfusion rates, discharge to home and mortality will be evaluated. Any signs of acute adverse reactions will be recorded at each visit during treatment.
Discussion: Although an evidence-based process using the best available literature and Delphi expert-opinion method has been used to establish an ERAS pathway of integrating TCM with western medicine. But, there is a lack of consensus about its effectiveness. This trial will provide convincing evidence about the effect of ERAS pathway
Trial registration: Registered on 12 Oct 2019; Trial number is ChiCTR190t0026487
Keywords: intertrochanteric fracture; enhanced recovery after surgery; perioperative period; integrating TCM with and western medicine; randomized controlled trial
Walking in the Hibbot, an innovative walking aid improves gait characteristics in children with cerebral palsy: a cross-sectional study
Authors: Hallemans Ann , Cuppers Ria , Truijen Steven , Ego Frank , Moens Marian , Wenmakers Dirk , Caers Philippe , Lebeer Jo 
Published: January 2020

Abstract
Background: Crouch gait is a common gait impairment in children with cerebral palsy (CP). Growing up and gaining weight, lever arm dysfunctions further deteriorate and walking efficiency progressively worsens over time with risk of losing ambulant capacity in adolescent life. The purpose of this study is to evaluate whether walking in an innovative walking aid can reduce the flexed gait pattern by improving alignment of the lower extremity joints and improving weight bearing. Methods: A semi-robotic walking aid called “Hibbot has been designed, which can be used in rehabilitation and daily-life settings. In a cross-sectional study, five children with CP, aged 3-7 years, were familiarized with the Hibbot, by using the walking aid for 30 minutes, twice a week during 3 months under the supervision of their physiotherapists, who were instructed how to use the Hibbot. Using 3D gait analysis kinematics of the lower limb and trunk as well peak vertical ground reaction forces were compared between walking in their habitual (or no) walking aid and walking in the Hibbot, using a paired samples t-test (p < 0.05, spm1d.org). Results: After the familiarization period of 3 months, a significant improvement in hip extension (mean difference 9.4 ± 1.4°) and knee extension (mean difference 10.1 ± 0.5°) during stance was observed when walking in the Hibbot compared to habitual walking. Improvements were also observed in trunk posture. However, differences in peak forces between habitual walking and walking in the Hibbot could not be confirmed. Conclusions: These results are promising as to the possibility of the Hibbot walking aid in counteracting a flexed gait pattern. Further research is necessary to investigate the net joint moments and muscle forces explaining the higher knee and hip extension and improved trunk posture while walking in the Hibbot. Furthermore research should focus on determining which children with CP would benefit most, what are contraindications and what dose effects are to be expected. Trial Registration: ClinicalTrials.gov, NCT04172324. Registered November 21 st , 2019 - Retrospectively registered. https://clinicaltrials.gov/ct2/show/NCT04172324
Psychometric Evaluation of Persian Version of the Prenatal Health Behavior Scale
Authors: ghiasvand malihe , Ozgoli Giti , kariman Nourossadat 
Published: January 2020

Abstract
Objective Prenatal behavior, including nutrition, exercise, drug use and smoking, affects health of a pregnant woman and her fetus. Psychometric evaluation of the Prenatal Health Behavior Scale (PHBS) is important for developing research on measurement of such this behavior during pregnancy. Methods The present descriptive study was conducted in 2018. The original version was in English, and was then translated into Persian after obtaining permission from tool designer and using Backward-Forward translation.The final Persian version included 240 pregnant mothers who were selected using random cluster sampling from hospitals affiliated to Shahid Beheshti University of Medical Sciences in Tehran. The factor analysis methods (exploratory and confirmatory), Pearson correlation, and Cronbach's alpha coefficient by SPSS V19 and LISREL V8.80 were used to analyze research findings. Results Content validity and face validity were assessed by survey of 10 experts and 20 pregnant mothers.In general, the CVI was 94%.The confirmatory factor analysis indicated that all factor loadings were appropriate and significant in the initial model. Furthermore, coefficient alpha was 0.932 for prenatal health promoting behavior and 0.935 for Health-impairing behaviors. The values were acceptable and indicated the high validity of tool in the Iranian sample. Conclusion The research findings indicated that the Prenatal Health Behavior Scale (PHBS) had acceptable psychometric properties among Iranian pregnant women and could be used as a valid tool for assessing prenatal health behavior in Iran.
Prediction of a new fracture after a wrist fracture – a five year follow up
Authors: Hansson Eva Ekvall , Dahlberg Leif E , Magnusson Måns , Beckman Anders 
Published: January 2020

Abstract
Background Falls and ensuing fractures are major challenges in our ageing population. The aim of this study was to study if clinical balance measures, function of the inner ear, self-rated health or fracture risk assessed by FRAX ® could predict future admission to hospital because of a fracture among a group of older persons with previous wrist fracture. Methods This was a longitudinal study with a 5-year follow-up. Searches in the local health authority’s patient administrative system (PAS) were performed 5 years after inclusion and baseline measurements were taken. Information was extracted about whether participants had been treated for a fracture or hospitalized other reasons during the 5-year period. Persons, 50 years and above, with previous wrist fracture (n=83). Five different clinical balance measures was assessed, postural sway was assessed by means of a force plate, vestibular asymmetry was assessed with the head- shake test, self-rated health by EuroQol 5 Dimension visual analogue scale and risk of future fracture by the Fracture Risk Assessment Tool (FRAX ® ). Age and body mass index was also used in the risk analysis. Results Age was associated with risk of future fracture, OR 1,06 (95% CI 1,01-1,12). The ability to stand on one leg with eyes open correlated significantly with future fracture (p=0.011) and so did FRAXosteo, however on the limits of significance (p=0.052). Conclusion This follow-up study showed that the one-leg standing time-test was a stronger predictor for future facture within five-years after a wrist fracture than FRAX not including a measure of balance.
Nonstructural versus structural bone grafting in the treatment of lumbar spinal tuberculosis combined with single-stage posterior debridement and instrumentation: a retrospective cohort study
Authors: Zhao Zenghui , Li Jianxiao , Peng Qiqi , Zhu Yong , Luo Wei , Du Xing , Ou Yunsheng 
Published: January 2020

Abstract
Background Many types of bone grafting have been reported for successful use in achieving anterior column support and bone fusion after one stage posterior debridement in the treatment of lumbar spinal tuberculosis. However, none-structural bone grafting has rarely been studied. This study was aimed to identify the feasibility of none-structural bone grafting from comparing the advantages and disadvantages with structural bone grafting in the treatment of lumbar spinal tuberculosis. Patients and methods We retrospectively reviewed patients with lumbar spinal tuberculosis who had undergone none-structural (n=27) and structural (n=22) bone grafting after single-stage posterior debridement and instrumentation with at least 24 months of follow-up. Plain radiographs, magnetic resonance imaging, and computed tomography scans, as well as health-related outcomes, including Visual Analog Scale for back pain, the Oswestry Disability Index were collated before surgery and at follow-up. Results Both none-structural and structural bone grafting were associated with significant improvements in quality of life parameters, the laboratory tests and the Cobb angle of local kyphosis. A slight loss of Cobb angle correction was in both two groups, without any associated complications. The operation duration and blood loss in none-structural bone grafting group was significantly less. The bone fusion rate was higher in structural bone grafting group. There were three complications in the none-structural bone grafting and four complications in the structural bone grafting group; the incidence of complications between the two groups was not significantly different. Conclusions Based on single-stage posterior debridement and pedicle screws fixation, none-structural bone grafting can achieve anterior column support with reducing surgical trauma, simplifying surgical procedure and decreasing intraoperative hemorrhage, but the lower rate of grafted bone fusion should be taken into consideration when choosing the aforementioned method. Key words Lumbar spinal tuberculous; Single-stage posterior debridement; Anterior column support; Bone fusion; None-structural bone grafting; Structural bone grafting
Cutaneous anthrax associated with handling carcasses of animals that died suddenly of unknown cause: Arua District, Uganda, January 2015–August 2017
Authors: Aceng Freda Loy , Ario Alex Riolexus , Alitubeera Phoebe Hilda , Kadobera Daniel , Sekamatte Musa , Okethwangu Denis , Bulage Lilian , Harris Julie R. , Nguma Willy , Ndumu Deo Birungi , Buule Joshua , Nyakarahuka Luke , Zhu Bao-Ping 
Published: January 2020

Abstract
Background Anthrax is a vaccine-preventable disease that can be transmitted to humans from infected animals. During May–June 2017, three persons with suspected cutaneous anthrax were reported in Arua District, Uganda; one died. All had recently handled carcasses of livestock that died suddenly. During July, a bull in the same community died suddenly. A skin lesion from the deceased person and a blood sample from the bull tested positive by PCR for Bacillus anthracis. The aim of this investigation was to establish the scope of the problem, identify exposures associated with illness, and recommend evidence-based control measures.Methods We defined a probable case as acute onset of a papulo-vesicular skin lesion subsequently forming an eschar in a resident of Arua District during January 2015–August 2017. A confirmed case was a probable case with a skin sample testing positive by polymerase chain reaction (PCR) for B. anthracis . We identified cases by medical record review and active community search. In a case-control study, we compared exposures between case-patients and frequency- and village-matched asymptomatic controls. We interviewed key animal health staff to learn about livestock deaths.Results We identified 68 case-patients (67 probable, one confirmed), and two deaths. Cases occurred throughout the three-year period, peaking during dry seasons. All cases occurred following sudden livestock deaths in the villages. Case-patients came from two neighboring sub-counties: Rigbo (attack rate (AR)=20.1/10,000 population) and Rhino Camp (AR=2.1/10,000). Males (AR=17/100,000) were more affected than females (AR=0.52/100,000). Persons aged 30-39 years (AR=26/100,000 population) were most affected. Among all cases and 136 controls, skinning (OR M-H =5.0, 95%CI: 2.3–11), butchering (OR M-H =22, 95%CI: 5.5–89), and carrying the carcass of livestock that died suddenly (OR M-H =6.9, 95%CI: 3.0–16) were associated with illness.Conclusions Exposure to carcasses of animals that died suddenly was a risk factor for cutaneous anthrax in Arua District during 2015-2017. We recommended public education about signs and symptoms of anthrax, prompt treatment of illness following exposure to animals that died suddenly, and safe disposal of carcasses of animals that die suddenly. In addition, the anthrax burden in livestock should be investigated to inform the cost-effectiveness of livestock vaccination.