bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of increasing the levels of insoluble fiber and on the method of diet formulation measures of energy and nutrient digestibility in growing pigs
Authors: Acosta Jesus A , Stein Hans H , Patience John F 
Published: April 2020

Abstract
The objective of this study was to determine the differences in response to distillers dried grains with solubles (DDGS) level under constant nutrient or floating nutrient concentrations. A total of 21 ileal-cannulated gilts (33.1 ± 0.4 kg body weight) were randomly allotted to one of seven dietary treatments in a 3-period incomplete Latin square design (n = 9). Treatments consisted of a 0% DDGS basal diet, plus diets containing 15%, 30%, or 45% DDGS. Diets were formulated using one of two different formulation methods: 1) constant nutrient (CNU) where nutrients were held equal to the basal diet or 2) constant ingredients (CIN) where DDGS were added at the expense of corn and all other ingredients remained constant, so nutrient levels were allowed to “float.” Chromic oxide was added to the diets at 0.5% as an indigestible marker. Increasing the level of DDGS decreased the apparent ileal digestibility (AID) of dry matter (DM), gross energy (GE), starch, dispensable amino acids (AA), and fiber components (P &lt; 0.050). The decrease in the AID of Lys, Met, Thr, and Trp was more pronounced under CNU compared with the CIN formulation method (P &lt; 0.050). The decrease in the AID of hemicellulose was less pronounced under CNU compared with the CIN formulation method (P = 0.045). There was a DDGS level × formulation method interaction for the AID of acid hydrolyzed ether extract (AEE; P = 0.015); for the CNU formulation method, increasing level of DDGS decreased the AID of AEE from 0% to 30% and remained similar from 30% to 45% DDGS, whereas the CIN had no effect on the AID of AEE. Increasing the level of DDGS decreased the apparent total tract digestibility (ATTD) of DM, GE, and fiber components (P &lt; 0.050), except for acid detergent fiber, which was not affected. The decrease in the ATTD of insoluble dietary fiber and total dietary fiber was less pronounced under CNU compared with CIN (P &lt; 0.050). The ATTD of AEE decreased for CNU compared with CIN (P &lt; 0.010). In conclusion, increasing the insoluble fiber level in the form of DDGS decreased the digestibility of most dietary components, including DM, GE, starch, insoluble fiber, and AA. The CNU and CIN formulation methods are equivalent when evaluating the digestibilities of DM, GE, starch, crude protein, and AA (when they were not added in purified synthetic forms). Differences between CNU and CIN formulation methods were detected for the digestibility of insoluble fiber, fat, and essential AA (when added as crystalline AA).
First arrivalQtomography based on an adjoint-state method
Authors: Huang Xinwei , Guo Zhenbo , Zhou Huawei , Yue Yubo 
Published: April 2020

AbstractUnder the assumption of invariant ray path in a weakly dissipative (high quality factor Q) subsurface medium, a tomographic inversion approach composed of two cascading applications of first arrival traveltime and Q tomography is proposed for compensating amplitude loss caused by near-surface anomalies, such as unconsolidated soils or the overburden gas cloud. To improve the computational efficiency, these two related tomography methods were adopted with an adjoint-state technique. First, arrival traveltime tomography will be performed to provide an inverted velocity model as one of the inputs for the following first arrival Q tomography. Then, the synthetic first break generated by the inverted velocity model will be used as a stable guidance of accessing the scopes of first arrival waveforms in the time domain where the potential attenuated time information is contained. The attenuated time will be estimated through a logarithmic spectral ratio linear regression corresponding to frequency-dependent propagation responses of different wave types. All these estimated attenuated times will be applied with reference signals to generate synthetic attenuated seismic data in the time domain, and their discrepancies with real data will be evaluated using similarity coefficients. The ones with larger values will be selected as optimal attenuated time inputs for the following Q tomographic inversion. Examples of both synthetic and field data reveal the feasibility and potential of this method.
Fundamentals of Anorectal Technical Skills: A Concise Surgical Skills Course
Authors: Kucera Walter B , Nealeigh Matthew D , Dyke Christopher , Ritter E Matthew , Artino Anthony R , Durning Steven J , Sweeney W Brian 
Published: April 2020

ABSTRACT

Introduction
Anorectal diseases, among the most common surgical conditions, are underrepresented in medical training. The Fundamentals of Anorectal Technical Skills course was developed to provide cost-effective formal training in diagnosis of common anorectal conditions and in commonly performed anorectal procedures using the theories of deliberative practice and perceptual and adaptive learning.

Materials and Methods
First- through third-year general surgery and internal medicine residents and third- and fourth-year medical students participated in a course consisting of didactic instruction and hands on skills stations. The course covered common anorectal conditions, including internal and external hemorrhoids, fissures, condylomata, abscesses, fistula-in-ano, rectal prolapse, pilonidal disease, pruritis ani, and anal and rectal cancer, as well as common procedures such as anoscopy, excision of thrombosed external hemorrhoids, banding of internal hemorrhoids, rigid proctoscopy, incision and drainage of an abscess, administration of local anesthesia, and reduction of rectal prolapse. Before the course, participants completed a questionnaire consisting of demographics; previous anorectal experience, as measured by procedural case volume; confidence diagnosing and treating anorectal conditions; and a clinical knowledge multiple-choice quiz. Immediately following the course, participants took an additional survey reassessing their confidence and testing their clinical knowledge. This study was granted an educational exception by the Institutional Review Board at Walter Reed National Military Medical Center.

Results
Forty-three learners participated in this course. Forty-six percent of participants had not participated in any anorectal cases, 26% had participated in 1 to 5 cases, 17% had participated in 6 to 10 cases, 6% had been involved with 11 to15 cases, and 6% had been involved with more than 15 cases. For learners who had no prior experience, 1 to 5 prior cases, or 6 to 10 cases, there were statistically and educationally significant increases in confidence for all diagnoses and procedures. Additionally, there were statistically and educationally significant increases between pre-course and post-course quiz scores for learners who had no prior experience (7.8 ± 2.0 vs. 11.8 ± 2.5, P &lt; 0.01, Cohen’s d = 1.8) and for those who had only participated in 1 to 5 cases (11.0 ± 3.7 vs. 14.2 ± 2.0, P = 0.04, Cohen’s d = 1.1). The changes in quiz scores for learners who previously had been involved with six or more cases were not statistically significant.

Conclusion
This course provides a cost-effective training that significantly boosts learners’ confidence in diagnosis of common anorectal procedures and confidence in performance of common anorectal procedures, in addition to improving objectively measured anorectal clinical knowledge.
An Attempt to Utilize the Body Composition Analyzer and the Functional Movement Screen (FMS) Test to Determine Injury Risk in Soldiers
Authors: Bertrandt Jerzy , Szarska Ewa , Łakomy Roman , Lepionka Tomasz , Anyżewska Anna , Lorenz Katarzyna , Maculewicz Ewelina 
Published: April 2020

Abstract

Introduction
Injuries are the most significant health problem of military services. Military medical surveillance data are useful for determining the magnitude and causes of the injury problem, identifying possible prevention targets and monitoring trends among military personnel. Soldiers serving in the Polish Armed Forces took part in the research. The aim of the study was to identify the most common injuries of the musculoskeletal system and to analyze causes of injuries. An additional goal was to examine whether there is a relationship between selected values of body composition indexes, the result of the functional movement screen (FMS) test and occurrence of an injury.

Material and Methods
The Polish Armed Forces soldiers serving in the land forces, military police, air forces took part in the research. The first stage was to complete the questionnaire regarding injuries in the last 5 years, their reasons, and the circumstances of their occurrence. After completing the survey, subjects were measured and weighed on the body composition analyzer. The next step was to perform a FMS to evaluate the locomotor system.

Results
In the groups of examined Polish soldiers, the most frequent injuries concerned lower limbs and, above all, a knee joint. The leading causes of injuries were activities resulting from the service as well as playing football and volleyball.

Conclusions
Parameters from the body composition analyzer such as body mass index, muscle mass distribution on lower limbs as well as evaluation in the FMS test may be helpful for the initial assessment of predisposition to injury.
Kinematic analysis of eagle simulations: evolution of λRe and its connection with mergers and gas accretion
Authors: Walo-Martín D , Falcón-Barroso J , Dalla Vecchia C , Pérez I , Negri A 
Published: April 2020

ABSTRACTWe have developed a new tool to analyse galaxies in the eagle simulations as close as possible to observations. We investigated the evolution of their kinematic properties by means of the angular momentum proxy parameter, λRe, for galaxies with M⋆ ≥ 5 × 109 M⊙ in the RefL0100N1504 simulation up to redshift two (z = 2). Galaxies in the simulation show a wide variety of kinematic features, similiar to those found in integral-field spectroscopic studies. At z = 0 the distribution of galaxies in the λRe–ε plane is also in good agreement with results from observations. Scaling relations at z = 0 indicate that there is a critical mass, Mcrit /M⊙ = 1010.3, that divides two different regimes when we include the λRe parameter. The simulation shows that the distribution of galaxies in the λRe–ε plane evolves with time until z = 2 when galaxies are equally distributed in both λRe and ε. We studied the evolution of λRe with time and found that there is no connection between the angular momentum at z = 2 and z = 0. All systems reach their maximum λRe at z = 1 and then steadily lose angular momentum regardless of their merger history, except for the high star-forming systems that sustain that maximum value over time. The evolution of λRe in galaxies that have not experienced any merger in the last 10 Gyr can be explained by their level of gas accretion.
Centrifugally driven mass-loss and outbursts of massive stars
Authors: Zhao Xihui , Fuller Jim 
Published: April 2020

ABSTRACT
Rotation and mass-loss are crucially interlinked properties of massive stars, strongly affecting their evolution and ultimate fate. Massive stars rotating near their break-up limit shed mass centrifugally, creating Be stars with circumstellar discs and possibly driving outbursts. Using the mesa stellar evolution code, we examine the effects of efficient angular momentum transport on the main-sequence and post-main-sequence rotational evolution of massive stars. In rapid rotators, angular momentum transported from the contracting core to the expanding envelope can spin-up the surface layers past the break-up rate, particularly for stars near (or beyond) the end of the main-sequence and in low-metallicity environments. We also demonstrate that centrifugal instabilities could arise in rapidly rotating massive stars, potentially triggering the S Doradus outbursts observed in luminous blue variable stars. Prior mass accretion from a binary companion increases both the likelihood and the intensity of centrifugal mass-loss. We discuss implications for massive stellar evolution, Be stars, and luminous blue variables.
Tidal evolution of exoplanetary systems hosting potentially habitable exoplanets. The cases of LHS-1140 b-c and K2-18 b-c
Authors: Gomes G O , Ferraz-Mello S 
Published: April 2020

ABSTRACT
We present a model to study secularly and tidally evolving three-body systems composed by two low-mass planets orbiting a star, in the case where the bodies rotation axes are always perpendicular to the orbital plane. The tidal theory allows us to study the spin and orbit evolution of both stiff Earth-like planets and predominantly gaseous Neptune-like planets. The model is applied to study two recently discovered exoplanetary systems containing potentially habitable exoplanets (PHE): LHS-1140 b-c and K2-18 b-c. For the former system, we show that both LHS-1140 b and c must be in nearly circular orbits. For K2-18 b-c, the combined analysis of orbital evolution time-scales with the current eccentricity estimation of K2-18 b allows us to conclude that the inner planet (K2-18 c) must be a Neptune-like gaseous body. Only this would allow for the eccentricity of K2-18 b to be in the range of values estimated in recent works (e = 0.20 ± 0.08), provided that the uniform viscosity coefficient of K2-18 b is greater than 2.4 × 1019 Pa s (which is a value characteristic of stiff bodies) and supposing that such system has an age of some Gyr.
On Spin dependence of the Fundamental Plane of black hole activity
Authors: Ünal Caner , Loeb Abraham 
Published: April 2020

ABSTRACT
The Fundamental Plane (FP) of black hole (BH) activity in galactic nuclei relates X-ray and radio luminosities to BH mass and accretion rate. However, there is a large scatter exhibited by the data, which motivated us for a new variable. We add BH spin as a new variable and estimate the spin dependence of the jet power and disc luminosity in terms of radio and X-ray luminosities. We assume the Blandford–Znajek process as the main source of the outflow, and find that the jet power depends on BH spin stronger than quadratically at moderate and large spin values. We perform a statistical analysis for 10 active galactic nuclei (AGNs) which have sub-Eddington accretion rates and whose spin values are measured independently via the reflection or continuum-fitting methods, and find that the spin-dependent relation describes the data significantly better. This analysis, if supported with more data, could imply not only the spin dependence of the FP relation, but also the Blandford–Znajek process in AGN jets.
Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins
Authors: Lindenburg Laurens , Huovinen Tuomas , van de Wiel Kayleigh , Herger Michael , Snaith Michael R , Hollfelder Florian 
Published: April 2020

Abstract
Site-saturation libraries reduce protein screening effort in directed evolution campaigns by focusing on a limited number of rationally chosen residues. However, uneven library synthesis efficiency leads to amino acid bias, remedied at high cost by expensive custom synthesis of oligonucleotides, or through use of proprietary library synthesis platforms. To address these shortcomings, we have devised a method where DNA libraries are constructed on the surface of microbeads by ligating dsDNA fragments onto growing, surface-immobilised DNA, in iterative split-and-mix cycles. This method—termed SpliMLiB for Split-and-Mix Library on Beads—was applied towards the directed evolution of an anti-IgE Affibody (ZIgE), generating a 160,000-membered, 4-site, saturation library on the surface of 8 million monoclonal beads. Deep sequencing confirmed excellent library balance (5.1% ± 0.77 per amino acid) and coverage (99.3%). As SpliMLiB beads are monoclonal, they were amenable to direct functional screening in water-in-oil emulsion droplets with cell-free expression. A FACS-based sorting of the library beads allowed recovery of hits improved in Kd over wild-type ZIgE by up to 3.5-fold, while a consensus mutant of the best hits provided a 10-fold improvement. With SpliMLiB, directed evolution workflows are accelerated by integrating high-quality DNA library generation with an ultra-high throughput protein screening platform.
Ubiquitin stimulated reversal of topoisomerase 2 DNA-protein crosslinks by TDP2
Authors: Schellenberg Matthew J , Appel C Denise , Riccio Amanda A , Butler Logan R , Krahn Juno M , Liebermann Jenna A , Cortés-Ledesma Felipe , Williams R Scott 
Published: April 2020

Abstract
Tyrosyl-DNA phosphodiesterase 2 (TDP2) reverses Topoisomerase 2 DNA–protein crosslinks (TOP2-DPCs) in a direct-reversal pathway licensed by ZATTZNF451 SUMO2 E3 ligase and SUMOylation of TOP2. TDP2 also binds ubiquitin (Ub), but how Ub regulates TDP2 functions is unknown. Here, we show that TDP2 co-purifies with K63 and K27 poly-Ubiquitinated cellular proteins independently of, and separately from SUMOylated TOP2 complexes. Poly-ubiquitin chains of ≥ Ub3 stimulate TDP2 catalytic activity in nuclear extracts and enhance TDP2 binding of DNA–protein crosslinks in vitro. X-ray crystal structures and small-angle X-ray scattering analysis of TDP2-Ub complexes reveal that the TDP2 UBA domain binds K63-Ub3 in a 1:1 stoichiometric complex that relieves a UBA-regulated autoinhibitory state of TDP2. Our data indicates that that poly-Ub regulates TDP2-catalyzed TOP2-DPC removal, and TDP2 single nucleotide polymorphisms can disrupt the TDP2-Ubiquitin interface.
The omics discovery REST interface
Authors: Dass Gaurhari , Vu Manh-Tu , Xu Pan , Audain Enrique , Hitz Marc-Phillip , Grüning Björn A , Hermjakob Henning , Perez-Riverol Yasset 
Published: April 2020

Abstract
The Omics Discovery Index is an open source platform that can be used to access, discover and disseminate omics datasets. OmicsDI integrates proteomics, genomics, metabolomics, models and transcriptomics datasets. Using an efficient indexing system, OmicsDI integrates different biological entities including genes, transcripts, proteins, metabolites and the corresponding publications from PubMed. In addition, it implements a group of pipelines to estimate the impact of each dataset by tracing the number of citations, reanalysis and biological entities reported by each dataset. Here, we present the OmicsDI REST interface (www.omicsdi.org/ws/) to enable programmatic access to any dataset in OmicsDI or all the datasets for a specific provider (database). Clients can perform queries on the API using different metadata information such as sample details (species, tissues, etc), instrumentation (mass spectrometer, sequencer), keywords and other provided annotations. In addition, we present two different libraries in R and Python to facilitate the development of tools that can programmatically interact with the OmicsDI REST interface.
The Multifunctional Dioxygenases of Gibberellin Synthesis
Authors: Lange Theo , Pimenta Lange Maria Jo�o 
Published: April 2020

Abstract
Gibberellin (GA) hormones regulate the development of plants and their responses to environmental signals. The final part of GA biosynthesis is catalyzed by multifunctional 2-oxoglutarate-dependent dioxygenases, which are encoded by multigene families. According to their enzymatic properties and physiological functions, GA-oxidases are classified as anabolic or catabolic enzymes. Together they allow complex regulation of the GA biosynthetic pathway, which adapts the specific hormonal needs of a plant during development and interaction with its environment. In this review, we combine recent advances in enzymatic characterization of the multifunctional GA-oxidases, in particular, from cucumber and Arabidopsis that have been most comprehensively investigated.
Interrelationships Between BDNF, Superoxide Dismutase, and Cognitive Impairment in Drug-Naive First-Episode Patients With Schizophrenia
Authors: Xiu Mei Hong , Li Zezhi , Chen Da Chun , Chen Song , Curbo Maile E , Wu Hanjing Emily , Tong Yong Sheng , Tan Shu Ping , Zhang Xiang Yang 
Published: April 2020

Abstract
The pathogenesis and etiology of schizophrenia (SCZ) remains unclear. Accumulating studies showed that complex interrelationships between brain-derived neurotrophic factor (BDNF) and an imbalanced redox system has a crucial role in the psychopathology of SCZ. However, the influence of the interrelationships of BDNF and superoxide dismutase (SOD) on cognitive impairment and clinical symptomatology in drug-naive first-episode (DNFE) SCZ patients has not been studied thoroughly. Serum BDNF levels, plasma total SOD, manganese-SOD (Mn-SOD), copper/zinc-containing SOD (CuZn-SOD) activities, and malondialdehyde (MDA) levels were measured in 327 DNFE patients with SCZ and 391 healthy controls. Cognitive functions were measured using the Repeatable Battery for the Assessment of Neuropsychological status (RBANS) and clinical symptoms were evaluated by the Positive and Negative Syndrome Scale (PANSS). Compared with the controls, the DNFE patients had increased activities of total SOD and CuZn-SOD, and reduced levels of BDNF and MDA. BDNF levels were positively correlated with CuZn-SOD activity in patients. In addition, we found that elevated Mn-SOD and CuZn-SOD activities were related to PANSS depression factor. Moreover, an interactive effect of BDNF levels and Mn-SOD activity was associated with attentional index score in the patients. Therefore, our findings suggested that interrelationships between BDNF and antioxidant mechanisms might underlie the pathological mechanisms of cognitive impairments and symptomatology in the DNFE patients with SCZ.
Antipsychotic Dose in Acute Schizophrenia: A Meta-analysis
Authors: Takeuchi Hiroyoshi , MacKenzie Nicole E , Samaroo Dominic , Agid Ofer , Remington Gary , Leucht Stefan 
Published: April 2020

AbstractLittle is known regarding optimal antipsychotic doses in the acute phase of schizophrenia. The aim of the present study was to employ the concept of minimum effective dose (MED) in examining efficacy and tolerability within this population. MED was identified for each antipsychotic through a previous systematic review. We then identified double-blind placebo-controlled randomized trials that involved fixed-dose antipsychotic monotherapy in acute schizophrenia and compared the identified MED vs higher doses of the same oral antipsychotic. Studies were selected from a recent meta-analysis examining dose–response relationship of second-generation antipsychotics and haloperidol. We extracted the data on study discontinuation, psychopathology, extrapyramidal symptoms, and treatment-emergent adverse events. For each antipsychotic, we conducted a meta-analysis to compare outcomes between MED and 2-fold MED, and MED and 3-fold MED. A total of 26 studies involving 5618 patients were included in the meta-analysis. In terms of study discontinuation, significant differences were found in study discontinuation due to lack of efficacy between MED and higher doses, in favor of 2-fold and 3-fold MEDs. Regarding psychopathology, both 2-fold and 3-fold MEDs were superior to MED for total and positive symptom scores. As for side effects, 2-fold MED proved inferior to MED for parkinsonism scores and diarrhea, whereas 3-fold MED was inferior for akathisia, somnolence, and vomiting. Findings suggest that clinicians can dose an antipsychotic at 2-fold or 3-fold MED for patients with acute schizophrenia but should closely monitor side effects.