bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of distal tibial osteotomy with distraction arthroplasty in varus ankle osteoarthritis
Authors: Nozaka Koji , Miyakoshi Naohisa , Kashiwagura Takeshi , Kasukawa Yuji , Saito Hidetomo , Kijima Hiroaki , Chida Shuichi , Tsuchie Hiroyuki , Shimada Yoichi 
Published: December 2019

Abstract
Background: Advanced to end-stage ankle osteoarthritis in highly active older individuals has traditionally been treated using tibiotalar arthrodesis. With tibiotalar arthrodesis, high levels of pain-free function are possible; however, there is a loss of ankle joint movement and a risk of future arthrosis in the adjacent joints. Distraction arthroplasty is a simple method that allows joint cartilage repair; however, the results are currently mixed, with some reports showing improved pain scores and others showing no improvement. Distal tibial osteotomy (DTO) without fibular osteotomy, a type of joint preservation surgery, has garnered attention in recent years. However, to our knowledge, there are no reports on DTO with joint distraction using a circular external fixator. Therefore, the purpose of this study was to examine the effect of DTO with joint distraction using a circular external fixator on ankle osteoarthritis. Methods: A total of 21 patients with medial ankle arthritis were examined. Arthroscopic synovectomy and a microfracture procedure were performed. Subsequently, angled osteotomy and correction of the distal tibia were performed. After ankle conditions improved, stabilization of the ankle joint was performed. An external fixator was used in all patients. In addition, joint distraction of about 5.8 mm was performed. All patients were allowed full weight-bearing walking immediately after surgery. Results: The anteroposterior mortise angle during weight-bearing, lateral mortise angle during weight-bearing, and talar tilt angle and anterior translation of the talus on ankle stress radiography significantly improved (P < 0.05). In addition, signal changes on magnetic resonance imaging improved in all patients. Visual analogue scale and American Orthopedic Foot & Ankle Society scores also improved significantly (P < 0.05). No severe complications were observed. Conclusion: DTO with joint distraction may be useful for older patients with a high physical activity level as a joint-preserving surgery for medial ankle osteoarthritis. Level of evidence: Level IV, retrospective case series Key words : distal tibial osteotomy, medial ankle arthritis, joint distraction, circular external fixator
Investigating dementia literacy among community-dwelling adults and older adults in Macau: A mixed methods study
Authors: Leung Angela YM , Leong Cindy S. U , Cheong Pak Leng , Tsang Alice S. M. , Nogueira Bernice O. C. Lam , Liu Ming , Van Iat Kio , Cheung Daphne , Xie Yao Jie , Loke Alice Yuen , Molassiotis Alex 
Published: December 2019

Abstract
Background: As population ageing and dementia incidence continue to increase worldwide, health systems are urged to empower the public to address factors related to dementia. This study aims to assess Macau citizens’ knowledge of dementia, attitudes towards persons with dementia, and help-seeking behaviours and intention toward dementia.Methods: This is a mixed-methods study with both quantitative (cross-sectional survey) and qualitative components (focus group interviews). The Alzheimer’s Disease Knowledge Scale (ADKS) and Dementia Attitude Scale (DAS) were utilized in the quantitative data collection, whereas focus group interviews were employed to gather context-specific understanding of dementia in the local setting. Descriptive statistics and Chi-square tests were used to analyze quantitative data, while content analysis was used for qualitative data.Results: The overall level of knowledge on dementia was low (ADKS mean total score = 17.38, SD = 3.31), with the least amount of knowledge in the caregiving domain. Meanwhile, attitudes toward persons with dementia was generally positive (mean [SD] = 89.07 [11.99]). Many of the focus group participants were also uncertain regarding the availability of dementia services in the region. The general public was interested to obtain dementia information through social media.Conclusions: Efforts should be made to build up dementia literacy in Macau, especially in terms of caregiving. Information about dementia could be disseminated in social media and by healthcare professionals.
Patient perspectives on treatment, care and rehabilitation after hip fracture: A meta-aggregative systematic review
Authors: Abrahamsen Charlotte , Nørgaard Birgitte 
Published: December 2019

Abstract
Background: Hip fracture surgery is a distressing and life-changing event for patients. The treatment, care and rehabilitation of hip fracture patients governed by evidence-based recommendations, the patients’ preferences are often not represented. The aim of this systematic review is to identify elderly hip fracture patients’ priorities during their care trajectory. Methods: A meta-aggregative approach was applied to present findings on patient perspectives. Data were extracted from the findings in the Results section of each study, categorized by similarity in meaning by both authors as either “health-related outcomes” or “healthcare-related experiences”. The quality of the presented evidence was evaluated, and all studies were assessed using the Critical Appraisal Skills Programme (CASP) checklist.Results: Sixteen qualitative studies met the inclusion criteria. The health-related outcomes category included: 1) symptoms and complications, 2) physical health, 3) mental health and 4) social relationships and 5) personal goals. Healthcare-related experiences revolved around: 1) waiting time, 2) information, 3) being treated with respect, 4) participation and 5) discharge.Conclusions: This systematic review provides an overview of hip fracture patients’ perspectives on important aspects of care, treatment and training during their trajectory, thereby contributing to the development of a patient-derived measure of hip fracture.
Trehalose increases jejunum cytoplasmic lipid droplets and suppresses adipocyte hypertrophy
Authors: Arai Chikako , Suyama Aki , Arai Shigeyuki , Arai Norie , Yoshizane Chiyo , Koya-Miyata Satomi , Mizote Akiko , Endo Shin , Ariyasu Toshio , Mitsuzumi Hitoshi , Ushio Shimpei 
Published: December 2019

Abstract
Background: Trehalose is a functional disaccharide that has anti-metabolic activities such as suppression of adipocyte hypertrophy in mice and alleviation of impaired glucose tolerance in humans. Trehalase hydrolyzes trehalose in the small intestine into two glucose molecules. In this study, we investigated whether trehalose can suppress adipocyte hypertrophy in mice in the presence or absence of trehalase. Methods: Trehalase knockout (KO) mice and wild-type (WT) mice were fed a high fat diet (HFD) and administered water with 0.3% (w/v) or without trehalose for 8 weeks. At the end of the experimental period, mesenteric adipose tissues and the small intestine were collected and the adipocyte size and proportion of cytoplasmic lipid droplets (CLDs, %) in jejunum epithelium were measured by image analysis. Results: Trehalose treatment was associated with suppressed adipocyte hypertrophy in both trehalase KO and WT mice. The rate of CLDs in the jejunal epithelium was increased in both trehalase KO and WT mice given water containing trehalose relative to untreated control mice. Since there was a negative correlation between jejunal epithelial lipid droplet volume and mesenteric adipocyte size, together with these results, trehalose treatment would suppress adipocyte hypertrophy. Because of jejunal epithelium containing lipid droplets falled into the intestinal lumen, triglyceride (TG) levels in feces tended to be higher in the KO/HFD/Tre group than in the KO/HFD/Water group. Whereas feces from trehalose-treated trehalase KO and WT mice tended to have more free fatty acids (FFA) than the untreated groups. Chylomicron-TG tended to be decreased in both trehalose-treated trehalase KO and WT mice. In vitro , addition of trehalose to differentiated Caco-2 cells increased intracytoplasmic lipid droplets and decreased secretion of the chylomicron marker ApoB48. Conclusions: The suppression of adipocyte hypertrophy in the presence and absence of trehalase indicates that trehalose mediates effects prior to being hydrolyzed into glucose. In both trehalase KO and WT mice, trehalose treatment increased the rate of CLDs in jejunal epithelium, reduced chylomicron migration from the intestinal epithelium to the periphery, and suppressed adipocyte hypertrophy. Thus, trehalose ingestion could prevent metabolic syndrome by trapping fat droplets in the intestinal epithelium and suppressing rapid increases in chylomicrons.
Comparative&nbsp;Mathematical&nbsp;Radiotherapy&nbsp;of&nbsp;Some&nbsp;Dog&nbsp;and&nbsp;Human&nbsp;Cancers
Authors: Saganuwan Saganuwan Alhaji 
Published: December 2019

Abstract
BackgroundCancer is a serious disease in human and canine species and can be managed using surgical, chemotherapeutic, immunotherapeutic and radiotherapeutic interventions. But for radiotherapy, determination of radiotherapeutic doses for specific cancer treatment is a serious problem both in medical and veterinary oncology.ResultsIn view of this, a number of formulas used in medical oncology has been adopted and applied for determination of effective radiotherapeutic doses especially for dogs. Findings revealed that the formulas could be used to estimate radiotherapeutic doses for a number of cancer diseases in dogs. More so, factors such as age, sex, type of cancer, location of cancer and biological effective dose should be considered. The most relevant formula is BED = nd [1 + (d)/(α/β)] where BED = biologically equivalent dose; d = dose per fraction; α/β = ratio for tumor (10) and n = number of fractions.ConclusionsMetronomic radiotherapy which may be defined as repetitive exposure of cytotoxic radio-active rays of < 2 Gy at regular and frequent intervals of 10-25 fractions of total 45 Gy may be safer for the treatment of malignancies in dog and human. But BED of 45 60 Gy has cancer control rate of 48 – 67%, and 50% probabilities of complications was caused by radiation rays of 54 Gy. Whereas a dose of > 2Gy in 2 fractions may improve quality of life and reduce chronic pain associated with terminal malignancies.
Expression of gallus epidermal growth factor (gEGF) with food-grade Lactococcus lactis expression system and its biological effects on broiler chickens
Authors: Zhou Yu , Chen Pinpin , Shi Shuai , Li Xiaowen , Shi Deshi , Zhou Zutao , Li Zili , Xiao Yuncai 
Published: December 2019

Abstract
Background Epidermal growth factor (EGF) is a multifunctional polypeptide, which could be utilized to solve problems in the industry such as increasing growth performance of commercial broilers or enhancing resistance to diseases under adverse conditions. Thus far, very few studies have focused on the gallus epidermal growth factor (gEGF) despite the availability of a plethora of studies on mammalian EGF. The aim of this study was to express gallus epidermal growth factor (gEGF) using a food-grade Lactococcus lactis expression system and to investigate its biological effects on broiler chickens. Results A recombinant Lactococcus lactis producing the secretary form of bioactive gEGF at 2.67 μg/ml in culture supernatant was generated. In vitro testing denoted that gEGF promoted the proliferation of UMNSAH/DF-1 cells. Sixty-three 5-day-old broiler chickens were divided into three groups and treated with either M17 medium (Control), supernatant of LL-pNZ8149 fermentation product (P-LL), or supernatant of LL-pNZ8149-gEGF fermentation product (gEGF-P-LL). Final body weight (FBW), average daily gain (ADG), and the gain:feed ratio of the gEGF-P-LL group were significantly (P<0.05) higher than those of the other groups during the two-week study. In addition, two weeks after therapy, the spleen and thymus indices, levels of serum IgA, IgG, and duodenum mucosal sIgA were all significantly (P<0.05) higher in the gEGF-P-LL group than in the other groups. Moreover, the villus height of the duodenum or jejunum and crypt depth of the jejunum of the gEGF-P-LL group were significantly (P<0.05) higher than those of the other groups, while the crypt depth of the duodenum showed the opposite pattern during the two-week study. Conclusions The bioactive gEGF was expressed with Lactococcus lactis expression system. Furthermore, gEGF has the potential to enhance growth performance, improve immune function, and promote intestinal development in broiler chickens. Keywords: gallus epidermal growth factor, Lactococcus lactis, broiler chickens, growth performance, immune function, intestinal development
Amblyomma hebraeum is the predominant tick species on goats in the Mnisi Community Area of Mpumalanga Province South Africa and is co-infected with Ehrlichia ruminantium and Rickettsia africae
Authors: Jongejan Frans , Berger Laura , Busser Suzanne , Deetman Iris , Jochems Manon , Leenders Tiffany , de Sitter Barry , Steen Francine van der , Wentzel Jeanette , Stoltsz Hein 
Published: December 2019

Abstract
Background: In sub-Saharan Africa, ticks of the genus Amblyomma are vectors of heartwater disease in domestic ruminants, caused by the rickettsial pathogen Ehrlichia ruminantium. Immature stages of these ticks often bite humans, whereby they act as vectors of tick-bite fever caused by Rickettsia africae. Moreover, Amblyomma ticks cause considerable damage to livestock due to their feeding behaviour. In South Africa, we studied the relative abundance of Amblyomma hebraeum ticks on goats of emerging farmers in Mpumalanga Province. A selected number of A. hebraeum nymphs and adult ticks was tested for co-infection with E. ruminantium and R. africae. Methods: A total of 630 indigenous goats, belonging to farmers in the Mnisi Community area, were examined for ticks in 2013 and 2014. All ticks were identified, and a selected number was tested by PCR with reverse line blot hybridisation (RLB).Results: In total, 13,129 ticks were collected from goats distributed over 17 different households. Amblyomma hebraeum was the predominant species collected, followed by Rhipicephalus microplus. Small numbers of Rhipicephalus appendiculatus, Rhipicephalus simus and Rhipicephalus zambeziensis were also identified. A. hebraeum was present throughout the year, with peak activity of adults in summer (November) and nymphs in winter (July). The ratio between adults and nymphs ranged from 1:2.7 in summer to 1:55.1 in winter. The mean prevalence of infection for E. ruminantium by PCR/RLB in adult ticks was 17.4% (31/178), whereas 15.7% (28/178) were infected with R. africae. In nymphs, 28.4% were infected with E. ruminantium and 38.8% carried R. africae infection. Co-infections of E. ruminantium and R. africae in adult and nymphal ticks were 3.9% (7/178) and 10% (14.9), respectively. Lameness of goats due to predilection of ticks for the interdigital space of their feet was observed in 89% of the households.Conclusions: Goats act as important alternative hosts for cattle ticks, which underscored the necessity to include goats in tick control programs. It is suggested to use acaricide-impregnated leg-bands as a sustainable method to kill ticks and prevent lameness in goats. The continuous challenge of goats by considerable numbers of E. ruminantium- infected ticks is a major obstacle for upgrading the indigenous goat breeds. Humans appear to be at risk to contract tick-bite fever in this area.
Prognostic Factors of the Postoperative Pancreatic Carcinoma and Implications for Identification of Potential Long-term Survivors
Authors: Hu Wei , Zhou Jiao , Zhou Wenbo , Wu Lun , Sun Shaohua , Zhang Zhen , Hu Hong-Sheng , Shen Feng 
Published: December 2019

Abstract
Background Patients with Pancreatic cancer (PC) have worse survival than patients with any other gastrointestinal malignancy. In present study, it is aim to investigate the prognostic factors of pancreatic carcinoma after curative resection . Methods 72 cases suffered from pancreatic carcinoma or periampullary carcinoma received curative, nine clinicopathologic factors that could possibly influence survival for postoperative mortality and overall survival were selected for univariate analysis and multivariate analysis using Cox proportional hazard mode. Results Univariate analysis showed that major factors of influence survival were size of the tumor, lymph node metastasis, and grade of differentiation (P<0.05). Multivariate analysis showed that lymph node metastasis and size of the tumor were the most important prognostic factors by multivariate analysis using the Cox proportional hazard model (P<0.01). Conclusions Prognostic factors of pancreatic carcinoma after resection are closed related to lymph node metastasis and the size of the tumor.
Hyperglycemia and hyperinsulinemia stimulate cervical cancer cell proliferation in vitro and in vivo study
Authors: Gutiérrez-Gutiérrez Miriam , Figueroa-Martínez Ishell Aline , Jurado Rafael , Uribe Norma , García-López Patricia , Rojas Jazmin Marlen Pérez 
Published: December 2019

Abstract
Background: Diabetes mellitus and malignant tumor are the second and third causes of women death in Mexico. Hyperglycemia, insulin and insulin-like growth factor 1 are the main risk factors involved in cancer development in patient with diabetes. The aim of this study was to evaluate the effect of hyperglycemia and hyperinsulinemia over cell proliferation and tumor growth in cervical cancer. Methods: Cell proliferation, apoptosis and cell cycle of cervical cancer cell lines (HeLa, SiHa and CaSki) in presence of hyperglycemia and/or insulin were evaluated. Xenograft model for cervical cancer was done in diabetic female nu/nu mice; biochemical parameters, body weight, tumoral volume and cell doubling time were evaluated. Results: Hyperglycemia and hyperinsulinemia significantly increase cell proliferation and decreases apoptosis with no change in cell cycle. Insulin treatment increase tumor volume and diminish cell doubling time, this group also developed hyperinsulinemia and in Langerhans pancreatic islet hypertrophy; whereas, hyperglycemic groups show the same effects but in lesser degree than the insulin treated group. Conclusion: Glucose and insulin stimulates both, proliferation and tumoral growth of cervical cancer, so this should be a possible explanation for the low survival of diabetic patients with cervical cancer in compare to non-diabetic patients with cervical cancer.
MiR-223 down-regulates RHOB to inhibit progression of papillary thyroid cancer
Authors: Zhang Wei , Ji Wenyue , Zhao Xudong 
Published: December 2019

Abstract
Backgrounds The incidence of thyroid carcinoma continues to increase every year. The incidence of papillary thyroid carcinoma (PTC) is the largest of all thyroid cancers, and is the most widely seen. Thus, Do some identification and testing of biomarkers related to tumorigenesis and development, which will help understand and treat PTC.Methods The expression of miR-223 and RHOB needs to be detected by polymerase chain reaction and western blot, respectively. Thyroid cell-line BCPAP and K1 cells were cultured, transfected for overexpression of miR-223or RHOB, and evaluated using MTT, Transwell, and apoptosis assays. In order to better understand the relationship between miR-223 and RHOB, dual luciferase reporter gene detection and RHOB rescue experiments were performed. In addition, clinical pathological parameters and correlation analysis of patients with miR-223 were also performed.Results Aberrant expression of miR-223 was detected in PTC samples. We also determined that miR-223 expression was associated with TNM staging and cervical metastasis. Moreover, miR-223 inhibits the increase in cell number and position, as well as defense against cancer cells and accelerates cell death. Furthermore, miR-223 excessive expression was associated with a significant inhibition of RHOB levels. According to the luciferase test report, it can be known miR-223 was able to bind RHOB 3′-UTR, it is possible to change its stability through these, and finally achieve the purpose of reducing RHOB. Finally,excessive expression of RHOB has undergone earth-shaking changes in the effect of miR-223 overexpression on cell number growth, invasion and disappearance.Conclusion Aberrant miR-223/RHOB expression took part in PTC make headway and miR-223 inhibition caused RHOB overexpression and cancer progression. Therefore, miR-223 may be a potential therapeutic target for PTC.
Social inequalities in body weight and dental caries experience among adolescents in Northern Norway: a cross-sectional study from the Tromsø Study Fit Futures cohort.
Authors: Stangvaltaite-Mouhat Lina , Furberg Anne-Sofie , Trovik Tordis A. 
Published: December 2019

Abstract
Background Non-communicable general and oral health conditions share common social determinants. The aim of the present study was to investigate and compare social inequalities in body weight (as a general health condition) and dental caries experience (as an oral health condition) based on several social determinants in a sample of adolescents from Northern Norway. Methods The present analysis included data from 477 girls and 508 boys from the population-based Tromsø study Fit Futures, which included first-year students attending upper secondary school in 2010-2011 from two municipalities in Norway (1038 participants in total, 93% participation rate). Multivariable binary logistic regression analysis stratified by sex was used to investigate the association between socioeconomic position (SEP) indicators (mother’s education, parents’ employment, and adolescent’s study program) and body weight (as a general health outcome), and dental caries experience (as an oral health outcome). Comparative models were adjusted for age, birth country, household composition (with or without adults), and a “composite health/health behavior” variable. Results Girls enrolled in the sports and physical education program (versus the vocational program) had lower odds of being overweight/obese (OR 0.20, 95% CI 0.04-0.96) and of having high dental caries experience (OR 0.26, 95% CI 0.10-0.67). For boys, demographic characteristic, i.e. living in a household without adults was strongly associated with being overweight/obese (OR=2.78, 95% CI 1.23-6.29) and having high dental caries experience (OR=2.29, 95% CI 1.08-4.84). Conclusions Social inequalities in body weight and dental caries experience might be expected only in adulthood, but our findings show that they were present as early as adolescence in Northern Norway, and that they were based on different social determinants for girls and boys. Similar magnitudes of social inequalities in body weight and dental caries experience among girls were observed based on the SEP indicator study program, and, among boys, on the demographic characteristic household composition. This suggests that the relationship between body weight and dental caries experience is based on socioeconomic and demographic factors rather than directly on general-oral health. Our findings support the broader concept of social context as a common risk factor for general and oral health.