bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression
Authors: Liu Yifei , Xie Pengfei , Jiang Daishang , Liu Jian , Zhang Jianguo , Bian Tingting , Shi Jiahai 
Published: December 2020

BackgroundEndoplasmic reticulum lipid raft-associated protein 2 (ERLIN2) is protein contained in the membrane of the endoplasmic reticulum. In lung adenocarcinoma (LUAD), the molecular function of ERLIN2 and the correlation between ERLIN2 and tumor-infiltrating immune cells have been unclear. The aim of our study was to determine the role of ERLIN2 in LUAD development to provide a better understanding of the molecular pathogenesis of this disease and identify new therapeutic targets for its treatment.MethodsImmunohistochemistry, Western blotting, and real-time quantitative polymerase chain reaction were used to detect protein and mRNA levels of ERLIN2 in LUAD and adjacent normal tissues. Using the A549, H1299 cell line, ERLIN2-short hairpin RNA was applied to silence ERLIN2 to determine its role in LUAD cell proliferation and invasion. Based on mRNA expression of ERLIN2 from the Cancer Genome Atlas (TCGA) database, we identified ERLIN2-related protein-coding genes and analyzed the Kyoto Encyclopedia of Genes and Genomes pathway to explore its potential biological functions and determined the correlation between ERLIN2 and tumor-infiltrating immune cells.ResultsERLIN2 was abnormally expressed in a variety of tumor tissues and is highly expressed in LUAD. This overexpression was associated with histological grade (P = 0.044), TNM stage (P = 0.01), and lymph node metastasis (P = 0.038). Patient overall survival was poorer with ERLIN2 overexpression. Downregulation of ERLIN2 inhibited LUAD cell proliferation and invasion in vitro. Based on mRNA expression of ERLIN2 from the TCGA database, 13 ERLIN2-related genes and 10 pathways were identified and showed a correlation between ERLIN2 and naive B cells and neutrophils.ConclusionERLIN2 could serve as a potential diagnostic and prognostic biomarker for LUAD and has demonstrated to be correlated with immune infiltrates, which suggests that it may represent a new therapeutic target for LUAD.
The Impact of SARS-CoV-2 Outbreak on Primary Sjögren's Syndrome: An Italian Experience
Authors: Carubbi Francesco , Alunno Alessia , Ferri Claudio , Gerli Roberto , Bartoloni Elena 
Published: December 2020

Objective:Since no data is available about the personal experience of people with primary Sjögren's syndrome (pSS) with regard to disease burden and management during the novel Severe Acute Respiratory Syndrome coronavirus (SARS-CoV)-2 outbreak, we aimed to explore these aspects with the ultimate goal to identify unmet needs and priorities.Methods:A telephone consultation was scheduled with patients with pSS and information regarding the disease status, ongoing treatment and symptoms/diagnosis of coronavirus disease 2019 (COVID-19) were collected. Clinical records were retrospectively evaluated to gather pre-COVID-19 information.Results:One hundred and two patients with pSS were contacted. Most rheumatology consultations and other pSS-related tests were canceled during the SARS-CoV-2 outbreak. Less than 30% of patients contacted the rheumatologist via telemedicine despite experiencing disease flares or therapy shortage. Disease activity and patient reported symptoms significantly worsened during the closure period. All patients practiced social distancing, most of those employed switched to smart working and different work settings impacted on the type of symptom worsening.Conclusion:This is the first study addressing the personal experience of pSS patients resulting from the impact of the SARS-CoV2 outbreak and it identifies unmet needs and priorities requiring to be addressed. Our findings may help designing individualized strategies
Diagnostic Strategies for Recurrent Cervical Cancer: A Cohort Study
Authors: Chao Xiaopei , Fan Junning , Song Xiaochen , You Yan , Wu Huanwen , Wu Ming , Li Lei 
Published: December 2020

ObjectiveThe effectiveness of various strategies for the post-treatment monitoring of cervical cancer is unclear. This pilot study was conducted to explore recurrence patterns in and diagnostic strategies for patients with uterine cervical cancer who were meticulously followed using a customized monitoring plan.MethodsThe epidemiological and clinical data of patients with recurrent cervical cancer treated from March 2012 to April 2018 at a tertiary teaching hospital were retrospectively collected. The diagnostic methods and their reliability were compared across patients with various clinicopathological characteristics and were associated with survival outcomes.ResultsTwo hundred sixty-four patients with recurrent cervical cancer were included in the study, among which recurrence occurred in the first three years after the last primary treatment in 214 patients (81.06%). Half of the recurrence events (50.76%) occurred only within the pelvic cavity, and most lesions (78.41%) were multiple in nature. Among all recurrent cases, approximately half were diagnosed based on clinical manifestations (n=117, 44.32%), followed by imaging examinations (n=76, 28.79%), serum tumor markers (n=34, 12.88%), physical examinations (n=33, 12.50%) and cervical cytology with or without high-risk human papillomavirus (hrHPV) testing (n=4, 1.52%). The reliability of the diagnostic methods was affected by the stage (p&lt;0.001), primary treatment regimen (p=0.001), disease-free survival (p=0.022), recurrence site (p=0.002) and number of recurrence sites (p=0.001). Primary imaging methods (sonography and chest X-ray) were not inferior to secondary imaging methods (computed tomography, magnetic resonance imaging and positron emission tomography-computed tomography) in the detection of recurrence. The chest X-ray examination only detected three cases (1.14%) of recurrence. Patients assessed with various diagnostic strategies had similar progression-free and overall survival outcomes.ConclusionsA meticulous evaluation of clinical manifestations might allow recurrence to be discovered in a timely manner in most patients with cervical cancer. Specific diagnostic methods for revealing recurrence were not associated with the survival outcomes.
Nomogram Predicting Survival to Assist Decision-Making of Metastasectomy in Patients With Metastatic Renal Cell Carcinoma
Authors: Wu Kan , Liu Zhihong , Shao Yanxiang , Li Xiang 
Published: December 2020

The survival benefit of metastasectomy (MSX) in patients with metastatic renal cell carcinoma (mRCC) remains unclear. A reliable model to predict an individuals’ risk of cancer-specific mortality (CSM) and to identify optimal candidates for MSX is needed. We identified 2,911 mRCC patients who underwent cytoreductive nephrectomy from the Surveillance, Epidemiology, and End Results database (2010–2015). Based on the Fine and Gray competing risks analyses, we created a nomogram to predict the survival of mRCC patients. Decision tree analysis was useful for patient stratification. The impact of MSX was assessed among three different subgroups. Overall, 579 (19.9%) cases underwent MSX. In the entire patients, the 1-, 2-, and 3-year cumulative incidence of CSM were 32.8, 47.2, and 57.9%, respectively. MSX was significantly associated with improved survival (hazard ratio [HR] = 0.875, 95% confidence interval [CI] 0.773–0.991; P = 0.015). Based on risk scores, patients were divided into three risk groups using decision tree analysis. In the low-risk group, MSX was significantly associated with a 12.8% risk reduction of 3-year CSM (HR = 0.689, 95% CI 0.507–0.938; P = 0.008), while MSX was not associated with survival in intermediate- and high-risk groups. We proposed a novel nomogram and patient stratification approach to identify suitable patients for MSX. The newly identified patient subgroup with a low-risk of CSM might benefit more from aggressive surgery. These results should be further validated and improved by the prospective trials.
Comparison of Manual and Semi-Automatic [18F]PSMA-1007 PET Based Contouring Techniques for Intraprostatic Tumor Delineation in Patients With Primary Prostate Cancer and Validation With Histopathology as Standard of Reference
Authors: Spohn Simon K. B. , Kramer Maria , Kiefer Selina , Bronsert Peter , Sigle August , Schultze-Seemann Wolfgang , Jilg Cordula A. , Sprave Tanja , Ceci Lara , Fassbender Thomas F. , Nicolay Nils H. , Ruf Juri , Grosu Anca L. , Zamboglou Constantinos 
Published: December 2020

PurposeAccurate contouring of intraprostatic gross tumor volume (GTV) is pivotal for successful delivery of focal therapies and for biopsy guidance in patients with primary prostate cancer (PCa). Contouring of GTVs, using 18-Fluor labeled tracer prostate specific membrane antigen positron emission tomography ([18F]PSMA-1007/PET) has not been examined yet.Patients and MethodsTen Patients with primary PCa who underwent [18F]PSMA-1007 PET followed by radical prostatectomy were prospectively enrolled. Coregistered histopathological gross tumor volume (GTV-Histo) was used as standard of reference. PSMA-PET images were contoured on two ways: (1) manual contouring with PET scaling SUVmin-max: 0–10 was performed by three teams with different levels of experience. Team 1 repeated contouring at a different time point, resulting in n = 4 manual contours. (2) Semi-automatic contouring approaches using SUVmax thresholds of 20–50% were performed. Interobserver agreement was assessed for manual contouring by calculating the Dice Similarity Coefficient (DSC) and for all approaches sensitivity, specificity were calculated by dividing the prostate in each CT slice into four equal quadrants under consideration of histopathology as standard of reference.ResultsManual contouring yielded an excellent interobserver agreement with a median DSC of 0.90 (range 0.87–0.94). Volumes derived from scaling SUVmin-max 0–10 showed no statistically significant difference from GTV-Histo and high sensitivities (median 87%, range 84–90%) and specificities (median 96%, range 96–100%). GTVs using semi-automatic segmentation applying a threshold of 20–40% of SUVmax showed no significant difference in absolute volumes to GTV-Histo, GTV-SUV50% was significantly smaller. Best performing semi-automatic contour (GTV-SUV20%) achieved high sensitivity (median 93%) and specificity (median 96%). There was no statistically significant difference to SUVmin-max 0–10.ConclusionManual contouring with PET scaling SUVmin-max 0–10 and semi-automatic contouring applying a threshold of 20% of SUVmax achieved high sensitivities and very high specificities and are recommended for [18F]PSMA-1007 PET based focal therapy approaches. Providing high specificities, semi-automatic approaches applying thresholds of 30–40% of SUVmax are recommend for biopsy guidance.
Mediating Effect of Self-Efficacy on the Relationship Between Medication Literacy and Medication Adherence Among Patients With Hypertension
Authors: Shen Zhiying , Shi Shuangjiao , Ding Siqing , Zhong Zhuqing 
Published: December 2020

Background: Studies have reported that medication literacy had a positive effect on medication adherence in patients with hypertension. However, little is known about the mechanism underlying this relationship in patients with hypertension.Objective: The purpose of this study was to investigate the mediating effect of self-efficacy between medication literacy and medication adherence.Methods: A total of 790 patients with hypertension were investigated using the Chinese Medication Literacy Scale for Hypertensive Patients (C-MLSHP), the Morisky Medication Adherence Scale-8 (MMAS-8) and the Medication Adherence Self-efficacy Scale-Revision (MASES-R). Hierarchical regression and the bootstrap approach were used to analyze the mediating effect of self-efficacy on the relationship between medication literacy and medication adherence.Results: A total of 60.9% of hypertensive patients were low adherent to their antihypertensive drug regimens. Self‐efficacy had a significant positive correlation with medication literacy (r= 0.408, p &lt; 0.001) and medication adherence (r = 0.591, p &lt; 0.001). Self-efficacy accounts for 28.7% of the total mediating effect on the relationship between medication literacy and adherence to antihypertensive regimens for hypertensive patients.Conclusion: More than half of the hypertensive patients in the study were low adherent to antihypertensive regimens. Self-efficacy had a partial significant mediating effect on the relationship between medication literacy and medication adherence. Therefore, it was suggested that hypertensive patients’ medication adherence might be improved and driven by increasing self-efficacy. Targeted interventions to improve patients’ self-efficacy should be developed and implemented. In addition, health care providers should also be aware of the importance of medication literacy assessment and promotion in patients with hypertension.
Changes in Efficacy Indicators for Adalimumab Biosimilar Candidate (HS016) for the Treatment of Active Ankylosing Spondylitis at Various Time Points
Authors: Su Jinmei , Li Mengtao , He Lan , Zhao Dongbao , Wan Weiguo , Liu Yi , Xu Jianhua , Xu Jian , Liu Huaxiang , Jiang Lindi , Wu Huaxiang , Zuo Xiaoxia , Huang Cibo , Liu Xiumei , Li Fen , Zhang Zhiyi , Liu Xiangyuan , Dong Lingli , Li Tianwang , Chen Haiying , Li Jingyang , He Dongyi , Lu Xin , Huang Anbin , Tao Yi , Wang Yanyan , Zhang Zhuoli , Wei Wei , Li Xiaofeng , Zeng Xiaofeng 
Published: December 2020

Objectives: A phase III, 24-weeks Chinese clinical trial demonstrated that efficacy and safety outcomes of treatments with 40 mg/0.8 ml HS016 (n = 416) or adalimumab (n = 232) for active ankylosing spondylitis (AS) patients was comparable. In the present study, a subanalysis of the clinical trial was conducted to determine whether also individual efficacy indicators were comparable between HS016 and adalimumab.Methods: The individual efficacy indicators total and nocturnal back pain, global assessment of disease activity, swollen joint count, Maastricht AS Enthesitis Score, Bath AS Disease Activity Index, Bath AS Functional Index, Bath AS Metrology Index and chest expansion, were assessed at baseline and every 2 weeks during the treatment period.Results: This subanalysis revealed no significant difference between the patient groups treated with HS016 or adalimumab for any individual efficacy indicator investigated at any time point (all p &gt; 0.05) beside faster total back pain score improvements in the adalimumab group on week 10, 12 and 22, which became equal at week 24. Among these indicators, chest expansion showed a significant increase at each time point compared with baseline, whereas all other efficacy indicators showed significant decreases compared with baseline at each time point (all p &lt; 0.05). All efficacy indicators had increased or decreased rapidly by week 2, and the values continued to increase/decrease up to week 12, with subsequent smaller changes thereafter up to week 24 of treatment.Conclusion: The response trajectory of most individual efficacy indicators was comparable between HS016 and adalimumab at each time point during the 24 weeks of the trial.Clinical Trial Registration:http://www.chictr.org.cn/showproj.aspx?proj=37910, identifier [ChiCTR1900022520]
Measurement of the Emission Spectra of Protein Solutions in the Infrared Range. Description of the Method and Testing Using Solution of Human Interferon Gamma as an Example
Authors: Penkov Nikita , Penkova Nadezda 
Published: December 2020

This paper describes a new method for measuring the spectra of infrared radiation emitted by protein solutions in the native state without any external excitation. Radiation is detected using a vacuum Fourier-transform infrared spectrometer, and the tested sample itself is a source of radiation. The necessary conditions for detecting radiation from a sample are the use of a highly sensitive cooled detector and the presence of a cold background. In this work, the background was a black body at the boiling point of nitrogen. It is also important to select the optimal vacuum pumping depth for the spectrometer and sample thickness. Radiation occurs due to spontaneous radiative transitions from excited vibrational energy states to the ground state of molecules. The intensity of radiation is determined by the population of the respective energy states, which, according to the Boltzmann distribution, depends on temperature and frequency. Using solution of human interferon gamma as an example, it has been shown for the first time that proteins have intrinsic radiation. The described method allows detecting spectral lines with a radiation power of about 10−8 W or even less. It has also been demonstrated that emission spectroscopy offers advantages in the signal-to-noize ratio compared to absorption spectroscopy and allows analyzing the structural characteristics of a protein, in particular, providing information about its secondary structure. Another significant advantage of the method described in the article is its noninvasiveness. At the sample temperature of 25°С, emission spectra can be detected in the range from 400 to 3,600 cm−1, which covers almost the entire frequency range of existing stretching and bending vibrations of molecules. At the same time, in the fingerprint region from 500 to 1,600 cm−1 (the most informative part of the infrared spectrum), the highest sensitivity of the method is demonstrated. There is also potential for extending the available frequency range into the far infrared and terahertz ranges. Being applicable to the study of protein solutions in low concentrations, the proposed approach is not only interesting from the point of view of fundamental science but also can have applied significance in biological and medical research.
The Relationship Between Homeownership and the Utilization of Local Public Health Services Among Rural Migrants in China: A Nationwide Cross-Sectional Study
Authors: Wang Zicheng , Wu Qiushi , Ming Juan 
Published: December 2020

Background: Rural–urban migrants frequently suffer from overrepresented health risks but have poor access to public health services. In China, homeownership status may play a vital role in obtaining local welfare. However, the relationship between homeownership and utilization of public health services has remained largely unexplored. This study aims to address the direct linkage between homeownership and utilization of local public health services among rural migrants in China.Methods: We applied the dataset from the 2017 National Migrants Population Dynamic Monitoring Survey (NMPDMS-2017) to explore the direct relationship between homeownership and the utilization of local public health services. Logit regression was conducted to discuss the associations and to explore the interaction effect.Results: The logit estimations reveal that homeownership is positively related to the establishment of a health record and participation in health education. The interaction term of homeownership and household location and the interaction between homeownership and healthcare center location are related to the increased establishment of a health record. However, the interaction of homeownership and household location merely reveals significant correlations with the health education model.Conclusion: Homeownership is positively associated with the utilization of local public health services among rural migrants in China. Furthermore, homeowners living in urban residential communities and within the vicinity of the healthcare center are more likely to access public health services than those living in other locations.
Quantitative SARS-CoV-2 Antibody Screening of Healthcare Workers in the Southern Part of Kyoto City During the COVID-19 Pre-pandemic Period
Authors: Fujita Kohei , Kada Shinpei , Kanai Osamu , Hata Hiroaki , Odagaki Takao , Satoh-Asahara Noriko , Tagami Tetsuya , Yasoda Akihiro 
Published: December 2020

Background: The coronavirus disease-2019 (COVID-19) pandemic is associated with a heavy burden on the mental and physical health of patients, regional healthcare resources, and global economic activity. While understanding of the incidence and case-fatality rates has increased, there are limited data concerning seroprevalence of antibodies against the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) in healthcare workers during the pre-pandemic period. This study aimed to quantitatively evaluate seroprevalence of SARS-CoV-2 antibodies in healthcare workers in the southern part of Kyoto city, Japan.Methods: We prospectively recruited healthcare workers from a single hospital between April 10 and April 20, 2020. We collected serum samples from these participants and quantitatively evaluated SARS-CoV-2 IgG antibody levels using enzyme-linked immunosorbent assays.Results: Five (5.4%), 15 (16.3%), and 72 (78.3%) participants showed positive, borderline, and negative serum SARS-CoV-2 IgG antibody status, respectively. We found the mean titer associated with each antibody status (overall, positive, borderline, and negative) was clearly differentiated. Participants working at the otolaryngology department and/or with a history of seasonal common cold symptoms had a significantly higher SARS-CoV-2 IgG antibody titer (p = 0.046, p = 0.046, respectively).Conclusions: Five (5.4%) and 15 (16.3%) participants tested positive and borderline, respectively, for SARS-CoV-2 IgG antibody during the COVID-19 pre-pandemic period. These rates were higher than expected, based on government situation reports. These findings suggest that COVID-19 had already spread within the southern part of Kyoto city at the early stage of the pandemic.
Становлення та удосконалення механізмів інноваційного розвитку державного управління в Україні
Authors: Sychenko Viktor , Martynenko Olga , Yakimenko Sergyi 
Published: December 2020

В статті досліджуються теоретичні підходи та практичний досвід становлення механізмів інноваційного розвитку державного управління в Україні. Зокрема, обгрунтовано підхід до формування механізмів інноваційного розвитку державного управління в країні. Крім того дослідження містить аналіз існуючих механізмів інноваційного розвитку державного управління. Розглянуто електронне врядування, як один з напрямів інноваційного розвитку електронного державного управління в державі. Уточнено класифікацію реальних проектів державно-управлінських інновацій.
Доведено що адміністративна реформа реалізується як концептуально-модельний процес, вона орієнтує трансформацію органів державної влади на реалізацію науково обґрунтованої моделі державного управління, що має конституюватися в результаті системних інновацій.
Показано що стратегія розвитку електронного урядування у контексті інноваційного процесу адміністративного реформування має полягати у проектуванні інформаційно-технологічних змін діяльності органів державної влади як механізму реалізації процесу переходу на модель публічного управління, і лише на цій основі вирішувати проблеми забезпечення доступності якісних державних послуг.
В статті доведено що науковці вважають метою інноваційної перебудови системи органів влади не просто галузеву ефективність, оптимізацію процесу прийняття і реалізації управлінських рішень, а «реалізацію основних функцій інноваційного державного управління», до яких насамперед доцільно віднести стратегічне планування і прогнозування; інтеграцію та координацію дій усіх суб’єктів, зацікавлених в інноваціях.
На основі проведеного дослідження визначено, що важливе значення має формування інноваційної інфраструктури, яку можна розглядати як інтегруючий механізм, що об’єднує в єдине ціле діяльність органів державної влади та інноваційні технології.