bluuebunny/crossref_metadata_2025_split huggingface dataset:
Currency's reversed marginal role in children's literature: Loans, debts, Mum Bucks and their subversion inDiary of a Wimpy Kid
Authors: Hui Haifeng 
Published: August 2020

AbstractMonetary issues abound in the best‐seller graphic novelDiary of a Wimpy Kid, where Heffley, his little brother Manny, and his friend Rowley often try to borrow or use others' money, the failure of which often develops into stealth or abuse of money, not to mention Heffley's mom's failed effort of disciplining him by introducing Mum Bucks. Money plays the role of currency and is one of the regulating and most fundamental principles in the adult society. Those who disregard the commonly accepted way of the use of money are often marginalized or even become criminals. On the contrary, in the children's world, money is often marginalized and destabilized—it is not respected with reference to its proper function in the adult world and it is often abused, counterfeited, or denied, the degree of which often indicates the degree of maturity of the character. This article analyzes different characters' regards and uses of money inDiary of a Wimpy Kidin an attempt to reveal the marginalized or even destabilizing role money plays in the children's world. It argues that the least mature character holds the most careless attitude toward money and this behavior often upsets the more mature character, who to a certain extent has partly internalized the central role of money in the adult world. It is, therefore, not surprising that Heffley's mom's introduction of Mom Bucks fail to work in the end because it symbolizes adults' futile enforcement of the disciplinary power of money in the children's world where it is not recognized as such.
Classifying rodent diets for comparative research
Authors: Verde Arregoitia Luis D. , D’Elía Guillermo 
Published: August 2020

AbstractThe foods consumed by animals reflect their adaptations and ecological roles, which makes diet an important trait for ecological, evolutionary, and paleobiological research. Rodents are the most species‐rich order of mammals and are also ecologically diverse, consuming a wide range of foods.To relate form with function, and to study diversification in the context of diet and feeding, researchers should follow classification schemes that capture diet composition.We evaluated existing diet datasets for mammals as well as previous classification schemes developed for rodents. To propose a suitable categorical scheme, we defined splits within plant‐eating and animal‐eating rodents, aiming to capture the intrinsic differences in diet composition in terms of the accessibility, nutrient content, and digestibility of various food items.Our proposal includes eight categories (Carnivore, Folivore, Frugivore, Granivore, Omnivore, Specialist Herbivore, Unknown, and Vermivore) in which species can be assigned with information from species accounts and field guides. To avoid ambiguity, we provide examples and detailed criteria for each diet category.For biologically tractable comparative research, we ask researchers to use our diet categories for rodent species. The categories can also be applied at other taxonomic levels or to different populations, sexes, or ontogenetic stages. We call for further study of the natural history of small mammals and the uptake of primary data into open databases.
Endogenous vertical structure with network externalities
Authors: Lee DongJoon , Choi Kangsik , Nariu Tatsuhiko 
Published: August 2020

AbstractThis study examines the endogenous vertical structure in which each manufacturer sells its product to its exclusive retailer who sells network goods to consumers (i.e. a duopoly in the upstream market) under Bertrand competition and Cournot competition with network externalities. We show that with strong (weak) network externalities under Bertrand competition, (a) it is a dominant strategy for each manufacturer to integrate (separate) its retailer; (b) with strong network externalities, the manufacturers’ profits, consumers’ surplus and social welfare are higher under vertical integration than under vertical separation. Under Cournot competition, (a) vertical separation is a unique subgame perfect Nash equilibrium; (b) with strong network externalities, the manufacturers’ profits, consumers’ surplus and social welfare are higher under vertical separation than under vertical integration.
Differential effects of a school‐based obesity prevention program: A cluster randomized trial
Authors: Nickel Nathan C. , Doupe Malcolm , Enns Jennifer E. , Brownell Marni , Sarkar Joykrishna , Chateau Dan , Burland Elaine , Chartier Mariette , Katz Alan , Crockett Leah , Azad Meghan B. , McGavock Jon M. , Santos Robert 
Published: August 2020

AbstractSchool‐based healthy living interventions are widely promoted as strategies for preventing obesity. The peer‐led Healthy Buddies™ curriculum has been shown to improve obesity‐related outcomes in school‐aged children. We examined whether these improvements existed among subgroups of children stratified by sex, income level and urban/rural geography. In a cluster‐randomized controlled trial, elementary schools in Manitoba, Canada, were randomly allocated to Healthy Buddies™ (10 schools, 340 students) or standard curriculum (10 schools, 347 students). Healthy Buddies™ participants had 21weekly lessons on healthy eating, physical activity and self‐efficacy, delivered by children age 9–12 to children age 6–8. We assessed pre‐ and post‐intervention body mass index (BMI) z‐scores, waist circumference, healthy living knowledge, dietary intake and self‐efficacy among the younger children. Compared to standard curriculum (n = 154), Healthy Buddies™ participants (n = 157) experienced a greater reduction in waist circumference (−1.7 cm; 95% confidence interval [CI][−2.8, −0.5 cm]) and improved dietary intake (4.6; 95% CI [0.9, 8.3]), healthy living knowledge (5.9; 95% CI [2.3, 9.5]) and self‐efficacy (5.3; 95% CI [1.0, 9.5]) scores. In subgroup analyses, effects for waist circumference (−2.0 cm; 95% CI [−3.6, −0.5]), healthy living knowledge (9.1; 95% CI [4.4, 13.8]) and self‐efficacy (8.3; 95% CI [3.3, 13.3]) were significant among boys. Dietary intake (10.5; 95% CI [5.5, 15.4]), healthy living knowledge (9.8; 95% CI [4.5, 15.0]) and self‐efficacy (6.7; 95% CI [0.7, 12.7]) improved among urban‐dwelling but not rural‐dwelling children. Healthy Buddies™ was effective for boys and children living in urban settings. Enhanced curricula may be needed to improve program effectiveness for select subgroups of school‐aged children.
Overweight in childhood of exclusively breastfed infants with a high weight at 5 months
Authors: Morgen Camilla S. , Larsson Melanie W. , Ängquist Lars , Sørensen Thorkild I. A. , Michaelsen Kim F. 
Published: August 2020

AbstractHigh infant weight increases the risk of childhood overweight, while breastfeeding may reduce the risk. However, some infants have a very high weight gain even though they are exclusively breastfed. We examined the risk of a high body mass index (BMI) and overweight in childhood for infants ≥2.5 SD above the median weight‐for‐age (WAZ) at age 5 months according to duration of exclusive breastfeeding (≤2, >2 to <4 or ≥4 months). The study is based on 13,401 7‐year‐old and 9,819 11‐year‐old children enrolled into the Danish National Birth Cohort (born 1997–2003). Linear and logistic regression analyses were used to examine the associations while adjusting for presumed confounders including birth weight. The results showed that infants ≥2.5 SD at 5 months, breastfed exclusively ≤2, >2 to <4 or ≥4 months had adjusted odds ratios (ORs) for overweight at age 7 at 3.67 (95% confidence interval [CI] [2.10, 6.43]), 3.42 (95% CI [2.32, 5.04]) and 3.19 (95% CI [1.90, 5.36]) respectively, when compared with infants <2.5 SD WAZ exclusively breastfed ≥4 months. The corresponding results for BMIz‐scores were 0.82 (95% CI [0.60, 1.04]), 0.63 (95% CI [0.48, 0.78]) and 0.57 (95% CI [0.38, 0.77]). For the ≥2.5 SD infants, the differences in risk of overweight and BMI according to duration of exclusive breastfeeding were neither significantly different among the 7‐year nor among the 11‐year‐old children. A high infant weight increases the odds of overweight and is associated with a higher BMI in childhood. Whereas the odds and BMIz‐scores tended to be lower for those exclusively breastfed longer, the differences were not statistically significant.
Population collapse in viviparid gastropods of the Lake Victoria ecoregion started before the Last Glacial Maximum
Authors: Van Bocxlaer Bert , Clewing Catharina , Duputié Anne , Roux Camille , Albrecht Christian 
Published: August 2020

AbstractEcosystems of Lake Victoria and riparian communities have been strongly disrupted by the introduction of the invasive Nile perch and its fishing industry. Beyond this invasion and other recent anthropogenic stressors, the Lake Victoria ecoregion also underwent phases of pronounced aridity over the Late Pleistocene, lastly during the Last Glacial Maximum (LGM). The consequences of recent and historic environmental change have been canvassed for the adaptive radiation of haplochromine cichlids occupying the ecoregion, but their effect on freshwater invertebrate diversity remains largely unknown. Here, we use 15 microsatellite loci and approximate Bayesian computation to test whether viviparid gastropods experienced a population bottleneck during the LGM, as did cichlids. Clustering analyses support three viviparid gene pools in the Lake Victoria ecoregion, gathering specimens from 1) Lake Albert and the White Nile, 2) the Victoria Nile and Lake Kyoga and 3) Lake Victoria and tributaries. The last group contains the highest genetic diversity, but all groups have a considerable number of private alleles and are inferred to predate the LGM. Examinations of demographic history reveal a 190‐ to 500‐fold population decline that started ~ 125–150 ka ago, thus substantially before the LGM bottleneck documented in haplochromine cichlids. Population collapses in viviparids are an order of magnitude more severe than declines in cichlids and have not been halted by the re‐establishment of freshwater ecosystems since the LGM. Recent anthropogenic ecosystem deterioration is causing homogenization of previously diversified microhabitats, which may contribute to (local) extinction and enhanced gene flow among species within gene pools.
Instability of natural selection at candidate barrier loci underlying speciation in wood ants
Authors: Kulmuni Jonna , Nouhaud Pierre , Pluckrose Lucy , Satokangas Ina , Dhaygude Kishor , Butlin Roger K. 
Published: August 2020

AbstractSpeciation underlies the generation of novel biodiversity. Yet, there is much to learn about how natural selection shapes genomes during speciation. Selection is assumed to act against gene flow at barrier loci, promoting reproductive isolation. However, evidence for gene flow and selection is often indirect and we know very little about the temporal stability of barrier loci. Here we utilize haplodiploidy to identify candidate male barrier loci in hybrids between two wood ant species. As ant males are haploid, they are expected to reveal recessive barrier loci, which can be masked in diploid females if heterozygous. We then test for barrier stability in a sample collected 10 years later and use survival analysis to provide a direct measure of natural selection acting on candidate male barrier loci. We find multiple candidate male barrier loci scattered throughout the genome. Surprisingly, a proportion of them are not stable after 10 years, natural selection apparently switching from acting against to favouring introgression in the later sample. Instability of the barrier effect and natural selection for introgressed alleles could be due to environment‐dependent selection, emphasizing the need to consider temporal variation in the strength of natural selection and the stability of the barrier effect at putative barrier loci in future speciation work.
Detoxification of plant defensive glucosinolates by an herbivorous caterpillar is beneficial to its endoparasitic wasp
Authors: Sun Ruo , Gols Rieta , Harvey Jeffrey A. , Reichelt Michael , Gershenzon Jonathan , Pandit Sagar S. , Vassão Daniel G. 
Published: August 2020

AbstractPlant chemical defences impact not only herbivores, but also organisms in higher trophic levels that prey on or parasitize herbivores. While herbivorous insects can often detoxify plant chemicals ingested from suitable host plants, how such detoxification affects endoparasitoids that use these herbivores as hosts is largely unknown. Here, we used transformed plants to experimentally manipulate the major detoxification reaction used by Plutella xylostella (diamondback moth) to deactivate the glucosinolate defences of its Brassicaceae host plants. We then assessed the developmental, metabolic, immune, and reproductive consequences of this genetic manipulation on the herbivore as well as its hymenopteran endoparasitoid Diadegma semiclausum. Inhibition of P. xylostella glucosinolate metabolism by plant‐mediated RNA interference increased the accumulation of the principal glucosinolate activation products, the toxic isothiocyanates, in the herbivore, with negative effects on its growth. Although the endoparasitoid manipulated the excretion of toxins by its insect host to its own advantage, the inhibition of herbivore glucosinolate detoxification slowed endoparasitoid development, impaired its reproduction, and suppressed the expression of genes of a parasitoid‐symbiotic polydnavirus that aids parasitism. Therefore, the detoxification of plant glucosinolates by an herbivore lowers its toxicity as a host and benefits the parasitoid D. semiclausum at multiple levels.
Captive environment influences the composition and diversity of fecal microbiota in Indo‐Pacific bottlenose dolphins, Tursiops aduncus
Authors: Suzuki Akihiko , Akuzawa Koyuki , Kogi Kazunobu , Ueda Keiichi , Suzuki Miwa 
Published: August 2020

AbstractCaptive environments impact the microbiota of captive animals; however, the comparison of microbiota between wild and captive dolphins has been poorly investigated. To explore the impact of a captive environment, we characterized the fecal microbiota of nine wild and four captive Indo‐Pacific bottlenose dolphins, Tursiops aduncus, using a next‐generation sequencing and revealed differences in the fecal microbiota between the analyzed groups. Statistical differences in abundances of the phyla Firmicutes and Proteobacteria were found between the wild and captive dolphins. Thirty‐six genera (22.9% of the total genera detected in all dolphins) were shared between the groups, whereas 79 (50.3%) and 42 (26.8%) genera were found only in the wild or captive dolphins, respectively. Several pathogenic bacterial genera, including Morganella and Mycoplasma, were detected only in the captive dolphins, and the genus Lactobacillus was found only in the wild dolphins. LefSe and SIMPER analyses revealed that the genus Clostridium sensu stricto 1 was significantly more abundant in the captive dolphins than in the wild ones and contributed the most to the dissimilarity of fecal microbiota between the groups. Our results indicate that the captive environment impacts the fecal microbiota of dolphins and reinforces the importance of monitoring potentially pathogenic bacteria in captivity.
In vitro activities of antifungal drugs against a large collection of Trichophyton tonsurans isolated from wrestlers
Authors: Kermani Firoozeh , Javidnia Javad , Hedayati Mohammad T. , Abastabar Mahdi , Haghani Iman , Didehdar Mojtaba , Fami Zaghrami Mahmoud , Shokohi Tahereh 
Published: August 2020

SummaryBackgroundTrichophyton tonsurans is the most common agent causing tinea gladiatorum in wrestlers, and limited data on susceptibility profiles of Trichophyton tonsurans are available.ObjectivesWe aimed to assess the in vitro activity of the common antifungal drug against a large collection of T tonsurans.Materials/MethodsThe in vitro activities to eight common antifungal drugs (sertaconazole, itraconazole, clotrimazole, fluconazole, butenafine, tolnaftate, terbinafine and griseofulvin) against 128 clinical isolates of T tonsurans strains, obtained from wrestlers with dermatophytosis, were performed according to CLSI M38‐A2 broth microdilution document.ResultsThe geometric mean minimum inhibitory concentration was the lowest for tolnaftate (0.022 µg/mL), followed by itraconazole (0.026 µg/mL), terbinafine (0.033 µg/mL), butenafine (0.088 µg/mL), griseofulvin (0.566 µg/mL), sertaconazole (2.875 µg/mL), clotrimazole (3.419 µg/mL) and fluconazole (12.540 µg/mL).ConclusionsEvaluation of antifungal susceptibility of dermatophytes showed that tolnaftate and itraconazole were the most effective drugs against T tonsurans and fluconazole had the least effect.
Toxoplasma gondii infection impairs the colonic motility of rats due to loss of myenteric neurons
Authors: Machado Camila Cristina Alves , Watanabe Paulo da Silva , Mendes Joana D’Arc de Lima , Pupim Andréia Carla Eugenio , Ortigoza Silvia Miyazaki , Bergoc Heloisa Gonçalves , Nino Beatriz de Souza Lima , Góis Marcelo Biondaro , Garcia João Luis , Blackshaw L. Ashley , Sant´Ana Débora de Mello Gonçales , Araújo Eduardo José de Almeida 
Published: August 2020

AbstractBackgroundToxoplasma gondii infection causes intestinal inflammation and diarrhea indicating possible intestinal motor dysfunction. Anatomical studies have shown alterations in the colonic myenteric plexus, but it is unknown whether this impacts motility and therefore whether motility is a target for treatment. We determined whether colonic coordinated movements are compromised by toxoplasmic infection and how it is associated with anatomical changes.MethodsMale Wistar rats were evaluated at 6, 12, 24, 48, and 72 hours and 30 days postinfection (dpi) and controls. Infected rats received orally 5 × 103 sporulated oocysts of strain ME‐49 (genotype II) of T gondii. The colon was collected for anatomical analysis (including the myenteric plexus immunolabeled with HuC/D, nNOS, and ChAT) and motility analysis in vitro (conventional manometry). Fecal output was measured daily.Key ResultsAt 12 hours postinfection, T gondii caused hypertrophy of the muscularis externa layer of the distal colon. There was loss of total, nitrergic, and cholinergic myenteric neurons in the proximal colon at 30 day postinfection (dpi); however, only loss of cholinergic neurons was found in the distal colon. Contractile complexes in the middle and distal colon were longer in duration in infected animals, which was associated with slower migration of the colonic motor complex. However, gastrointestinal transit time and fecal pellet output remained unchanged during the T gondii infection.Conclusions and InferencesToxoplasma gondii caused myenteric neuronal loss in the proximal and distal colon and altered the motility pattern in the middle and distal colon to a more propulsive phenotype.
Genome‐wide functional analysis of hot pepper immune receptors reveals an autonomous NLR clade in seed plants
Authors: Lee Hye‐Young , Mang Hyunggon , Choi Eunhye , Seo Ye‐Eun , Kim Myung‐Shin , Oh Soohyun , Kim Saet‐Byul , Choi Doil 
Published: August 2020

Summary

Plants possess hundreds of intracellular immune receptors encoding nucleotide‐binding domain leucine‐rich repeat (NLR) proteins. Full‐length NLRs or a specific domain of NLRs often induce plant cell death in the absence of pathogen infection.

In this study we used genome‐wide transient expression analysis to identify a group of NLRs (ANLs; ancient and autonomous NLRs) carrying autoactive coiled‐coil (CCA) domains in pepper (Capsicum annuum). CCA‐mediated cell death mimics hypersensitive cell death triggered by the interaction between NLRs and pathogen effectors.

Sequence alignment and mutagenesis analyses revealed that the intact α1 helix of CCAs is critical for both CCA‐ and ANL‐mediated cell death. Cell death induced by CCAs does not require NRG1/ADR1 or NRC type helper NLRs, suggesting ANLs may function as singleton NLRs. We also found that CCAs localize to the plasma membrane, as demonstrated for Arabidopsis singleton NLR ZAR1. Extended studies revealed that autoactive CCAs are well conserved in other Solanaceae plants as well as in rice, a monocot plant. Further phylogenetic analyses revealed that ANLs are present in all tested seed plants (spermatophytes).

Our study not only uncovers the autonomous NLR clade in plants but also provides powerful resources for dissecting the underlying molecular mechanism of NLR‐mediated cell death in plants.
Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana
Authors: Li Hongjiang , von Wangenheim Daniel , Zhang Xixi , Tan Shutang , Darwish‐Miranda Nasser , Naramoto Satoshi , Wabnik Krzysztof , De Rycke Riet , Kaufmann Walter A. , Gütl Daniel , Tejos Ricardo , Grones Peter , Ke Meiyu , Chen Xu , Dettmer Jan , Friml Jiří 
Published: August 2020

Summary

Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear.

Here, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze‐fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains.

Pharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall components as well as connections between the cell wall and the plasma membrane.

This study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems.
Gut–adipose tissue crosstalk: A bridge to novel therapeutic targets in metabolic syndrome?
Authors: Rosendo‐Silva Daniela , Matafome Paulo 
Published: August 2020

SummaryThe gut is one of the main endocrine organs in our body, producing hormones acknowledged to play determinant roles in controlling appetite, energy balance and glucose homeostasis. One of the targets of such hormones is the adipose tissue, a major energetic reservoir, which governs overall metabolism through the secretion of adipokines. Disturbances either in nutrient and metabolic sensing and consequent miscommunication between these organs constitute a key driver to the metabolic complications clustered in metabolic syndrome. Thus, it is essential to understand how the disruption of this crosstalk might trigger adipose tissue dysfunction, a strong characteristic of obesity and insulin resistance. The beneficial effects of metabolic surgery in the amelioration of glucose homeostasis and body weight reduction allowed to understand the potential of gut signals modulation as a treatment for metabolic syndrome‐related obesity and type 2 diabetes. In this review, we cover the effects of gut hormones in the modulation of adipose tissue metabolic and endocrine functions, as well as their impact in tissue plasticity. Furthermore, we discuss how the modulation of gut secretome, either through surgical procedures or pharmacological approaches, might improve adipose tissue function in obesity and metabolic syndrome.