bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microclonal propagation and callus induction of some species of Carlina L. genus
Authors: Kravets N. B. , Tulaidan N. V. , Mosula M. Z. , Drobyk N. M. 
Published: March 2020

Aim. The aim of the research was to choose the conditions for microclonal propagation and obtain callus cultures from Carlina аcaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl plants in vitro. Methods. For microclonal propagation of С. acaulis, C. cirsioides and C. onopordіfolia we used rosettes of 2–3-month specimens and planted them on semi-solid Murashige and Skoog (MS) medium with decreased macro- and microsalts concentrations (MS/2) supplemented with kinetin (Кin) (from 1–3 mg/l) and 0.1 mg/l of 1-naphthaleneacetic acid (NAA). For induction of callus formation, we used root, stem explants from С. acaulis, C. cirsioides and C. onopordіfolia, and planted them on nutrient media MS, MS/2, and Gamborg and Eveleigh (В5) supplemented with different concentrations of cytokinins – 6-benzylaminopurine (BAP) or Кin and auxins – 2.4-dichlorophenoxyacetic acid (2.4-D) or NAA and indole-3-acetic acid (IAA). Results. MS/2 medium supplemented with growth regulators of NAA and Кin were the most efficient to provide the formation of microclones. For C. сirsioides plants, this indicator was 6.6–6.8 rosettes per graft after 6 months of cultivation and for С. acaulis and C. onopordіfolia – 4.2–5.0 and 4.8–5.2 respectively. To raise the percentage of rooting for microclones of Carlina species, it was expedient to steep them preliminarily in the solution of indole-3-butyric acid (IBA) with 1000 mg/l concentration for a minute. Optimal for obtaining callus tissue from Carlina plants was nutrient medium MS supplemented with 3 mg/l IAA, 0.5 mg/l NAA and 0.5 mg/l Kin and MS/2 with 0.1 mg/l BAP and 0.5 mg/l 2.4-D; under such conditions the percentage of callus induction exceeded 90 % for all types of explants. Conclusions. There were chosen the conditions for microclonal propagation of С. acaulis, C. cirsioides and C. onopordіfolia and worked out the schemes for enrooting obtained microclones in vitro. Capable of growing rapidly callus cultures from root and stem explants of the investigated plant species were obtained.
Keywords: Carlina аcaulis L., Carlina cirsioides Klok, Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl, in vitro, microclonal propagation, callus induction.
Cloning of sequence of homologous crt-cluster in Streptomyces globisporus 1912-бп
Authors: Lukyanchuk V. V. , Polishchuk L. V. 
Published: March 2020

Aim. The aim was to set influence to the additional homologous crt-cluster on carotenogenesis of cells of streptomycete recipient. Methods. For this purpose transformation by the hybrid plasmid pWC 9,6 was conducted. This plasmid contained the fragment of the crt-cluster sequence (9576 bp) of Crt+-mutant S. globisporus Crt4 in a Crt--recipient S. globisporus 1912-бп. To construct this hybrid plasmid, a fragment of PLR-copies of sequence of the crt-cluster of mutant S. globisporus 1912 Crt4 was cloned in the shuttle vector pWHM4 (6.6 kb). Insertion was done into unique restriction sites for endonucleases XbaI and HindIII in a polylinker of this vector. These endonucleases have not restriction sites into the crt-cluster sequence. Results. The plasmid pWC 9,6 (16.2 kb) that contains the crt-cluster sequence (9576 bp) of the Crt+-variant Crt4 of the strain S. globisporus 1912 was constructed. The plasmid successfully functions in the cells of both recipients (E. coli XL1 Blue and S. globisporus 1912-бп). It provides to them resistance to the corresponding antibiotics. The plasmid pWC 9,6 stably keeps its molecular size (16.2 kb). However, indisputable proofs of expression of the crt-clusters in transformants were not got. Conclusions. The plasmid pWC 9,6, that is able to transform and stably function in the cells of both recipient microorganisms (Streptomyces and E. coli) was constructed.
Keywords: crt-cluster, shuttle vector, cloning, resistance, PCR.
Optimization of conteining dermal coverages production for burn wound treatment at in vivo model
Authors: Matsevych L. L. , Papuga A. Ye. , Ruban T. P. , Beregova T. V. , Lukash L. L. 
Published: March 2020

Aim. The aim was to estimate the influence of cell suspension quality on the therapeutic efficiency of cell-containing dermal coverages in animal model in vivo. Methods. We carried out the application of gel wound coverages with different quality of cellular compound on the third degree burns of ICR line mice. In the negative control group animals were treated with fresh medium-containing gel, and in positive control – by gel containing high quality cell suspension. Photo fixation of burn wound status was carried out once a day. The results were estimated by ANOVA approach. Results. There was a statistically significant difference of burn wounds development and healing between positive control and both experimental groups. The decreasing of alive cell fraction in prepared gel within 5 hours has been shown as well. Conclusions. It has been shown the dependence of wound healing properties of coatings containing cells on the cell compound quality, in particular, from the viability and integrity of cells. These results are important for developing of clinical protocols using such cell‑containing dermal equivalents.
Keywords: burn wound, dermal equivalent, stem cells, skin substitute, tissue engineering.
Cell selection with heavy metal ions for obtaining wheat and maize forms tolerant to osmotic stresses
Authors: Sergeeva L. E. , Bronnikova L. I. 
Published: March 2020

Aim. The obtaining tolerant to salt and water stresses wheat and maize variants via cell selection with barium and cadmium heavy metal cations were the idea. Barium cations disrupt the cell potassium transport. Cadmium cations affect the dehydrins. The maintenance of these parameters under stress conditions provides the plant tolerance. Methods. Modified media for initial selection were developed by the supplement of the lethal doses of heavy metal ions. Wheat and maize suspension cultures were plated on selective media. The relative fresh biomass growth was the marker of variant stress tolerance. Results. Ba-resistant and Cd‑resistant plant cell lines were obtained. Those variants were tested under salinity and water stress pressure at lethal for wild type cells doses. Cell lines demonstrated the combined tolerance to abiotic stresses. Conclusions. The cell selection with heavy metal ions – is a new approach for isolation cell variants with higher levels of osmotic stresses tolerance.
Keywords: cereals, osmotic stresses, cell selection with heavy metal ions, combined tolerance.
Assessment of structural peculiarities of glaziovianin A interaction with human α-, β and γ-tubulins
Authors: Karpov P. A. , Spivak S. I. , Rayevsky O. V. , Nyporko O. Yu. , Ozheredov S. P. , Blume Ya. B. 
Published: March 2020

Aim. To determine the features of the ligand-protein interaction of glaziovianin A and human α-, β- and γ-tubulin. Methods. Protein and ligand spatial structure modelling (I-Tasser, Grid), molecular docking (CCDC Gold), molecular dynamics simulation (GROMACS). Results. Using the method of molecular docking in CCDC Gold ligand-protein complexes of glaziovianin A and human α-, β- and γ-tubulin were reconstructed. Studied ligand interactions in GTP/GDP-exchange and colchicine binding sites of different tubulin isotypes. The built ligand-protein complexes were studied using molecular dynamics simulations. Conclusions. Binding of glaziovianin A with human tubulin was confirmed exposing its derivatives as perspective tubulin effectors. The binding energies of ligand-protein interaction confirm higher affinity for β-tubulin molecules, and it was suggested that glazovianin A binding may occur at two alternative sites: GTP/GDP-exchange site and site of colchicine binding.
Keywords: tubulin, glaziovianin A, binding, antitumor activity.
Evolution of the stochastic and structural type ecosystem
Authors: Mikhyeyev A. N. , Protasov A. A. 
Published: March 2020

The article attempts to apply a systematic approach to the consideration of types of existing systems to identify the specific evolution of ecosystems at different levels of organization. The conceptual main consideration of the problem of the specifics of the evolution of biotic systems at different levels of organization was the idea of the alternation of the types of their organization, namely, the statistical (C-system) and deterministic (D-system). For evolutionary transformations of C-systems (for example, populations), the influence of altered environmental factors is of primary importance, and for internal systems, for systems (for example, communities). The idea that the D-systems evolve according to the type of nomogenesis, and the C-system – selectogenesis is substantiated. Within the framework of the proposed isolation of different types of systems, the evolution of the biosphere probably proceeds according to the type of evolution of D-systems. Structural elements of the biosphere are diverse biogeocenoses, each of which represents a C-type system formed by the corresponding communities and elements of the environment.
Keywords: evolution, levels of organization, ecosystems, biosphere.
Genetic analysis of oleic acid сontent in the oil of maize on the basis of mutation Sugary-1
Authors: Tymchuk D. S. , Potapenko G. S. , Tymchuk N. F. , Myzhylko V. V. 
Published: March 2020

Aim. Genetic analysis of oleate content in the carriers of maize mutation su1. Methods. Experiments were conducted on a series of inbreds – carriers of mutation su1, as well as hybrids derived from their diallel and top cross crossings. Results. Inbreds – carriers of mutation su1 were notable as having the increased content of oleate, which was inherited by the type of incomplete dominance with the prevailing contribution of additive effects to the variance. Conclusions. Increasing the content of the oleic acid glycerides in the carriers of mutation su1 caused by the spatial coupling of the locus su1 with the oleate – encoding locus, the effect of which can be modified by the polygenic complex.
Keywords: Zea mays L., mutation su1, oleic acid, genetic analysis.
Susceptibility of siberian hybrids new races of zarazhi
Authors: Hablak S. G. , Abdullaeva Ya. A. , Ryabovol L. O. , Ryabovol Ya. S. 
Published: March 2020

Aim. A study of the racial composition of broomrape on sunflower crops in the northern part of the Steppe of Ukraine. Methods. Assessment of the resistance of hybrids and sunflower test lines to broomrape was carried out by a modified soil method. Results. The broomrape parasitizing on the fields of sunflower has a high degree of virulence, which overcomes the immunity of the best hybrids of domestic and foreign breeding, resistant to the E, F and G races of this parasite. Conclusions. The emergence of new very aggressive broomrape races (E, F and G) indicates the important need to solve the problem of creating breeding material resistant to new races of this parasitic plant. Intensive with the accumulation of parasite races E, F and G in the sunflower crops is associated with the disruption of crop rotations and the saturation of fields with hybrids of this culture, resistant mainly to 4 (D) and 5 (E) races of the parasite.
Keywords: Orobanche сumana Wallr., race, sunflower, hybrid, root system, root allocation, srigolactones.
Microsatellite alteration and metylation of RASSF1 gene in renal cell carcinoma
Authors: Dubrovska H. V. , Onyshchenko K. V. , Pereta L. V. , Kashparova O. V. , Grygorenko V. M. , Skrypkina I. Ya. 
Published: March 2020

Aim. It becomes more and more relevant to study as the promising diagnostic and prognostic markers the epigenetic and genetic changes that determine the malignant transformation of cells and biological behavior of tumors. The purpose of our research was to study the loss of heterozygosity of the RASSF1 gene and the status of methylation of the locus of the RASSF1A gene in malignant tumors of the kidneys and to assess the potential for its use as a marker in clinical practice. Methods. The determination of the allele imbalance in the RASSF1 gene locus was performed by PCR using highly polymorphic markers D3S966, D3S1568 for renal cancer (RC) in paired samples of tumor and conditionally normal tissue and the PCR products detection in polyacrylamide gels. The epigenetic variability analysis of the RASSF1A gene was performed by using methyl-specific PCR, which was preceded by bisulfite DNA treatment. The statistical significance of the differences between the study groups was analyzed by using F-test and the U-test. Results. Our studies showed the predictive value of inactivation of RASSF1A in RC. The methylation level of the genomic DNA of the RASSF1A loci of the RASSF gene was 72 %. The loss of the heterozygosity of the RASSF1 gene in this type of oncological disease was about 35.4 %. Conclusions. The results may indicate that the gene RASSF1can become a candidate for inclusion in the prognostic system of clinical course of the disease.
Keywords: kidney cancer, epigenetic changes, methylation, loss of heterozygosity, RASSF1.
Cytogenetic aspects instability to the genome in newborns from different ecological districts of Ivano-Frankivsk region
Authors: Kocherha Z. P. , Kovalchuk L. Ye. 
Published: March 2020

Aim. Оf the research work was to establish the frequency and spectrum of chromosomal aberrations, associations of acrocentric chromosomes in infants from different ecological regions of Ivano-Frankivsk region. Methods. Metaphase plates of 187 infants from different ecological districts have been analyzed with the aim to determine the frequency and spectrum of chromosomal aberrations, associations of acrocentric chromosomes in newborns. Results. The obtained results of the frequency of associations of acrocentric chromosomes correlated with the frequency indices of chromosomal aberrations (r correlated from 0.68 to 0.84), that proved the negative influence of ecological living conditions on immunogenetic status and adaptive human capabilities. Conclusions.It was determined that the total number of chromosomal aberrations in newborns from districts of ecological comfort was 2.0 and 2.3 times lower as compared to newborns from zones with chemical and radiation contamination. The frequency of cells with associations of acrocentric chromosomes was 7.8 % higher in children from zones of chemical contamination and 9.9 % from zones of radiation pollution as compared to the children from districts of ecological comfort.
Keywords: chromosomal aberrations, associations of acrocentric chromosomes, newborns, ecological zones.
EMT-mechanizm induces the leukemic stemness phenotype in myeloid leukemias
Authors: Shvachko L. P. 
Published: March 2020

Aim. To study the targeted expression EMT-induced markers N-cadherin, Snail and Twist in patients with the chronic and acute myeloid leukemias. Methods. RT-PCR, electroforesic in agarose gel, TotalLab v. 2.01 densitometry. Results. Have been investigated the relative levels of mRNA expression of N-cadherin and transcriptional factors Snail and Twist, associated with epithelial-to-mesenchymal induction (EMT) in patients with the essential polycytemia (EP), the chronic mieloid leukemia CML), the acute myeloid leukemia (AML) and the acute lymphoblastic leukemia (ALL). Conclusions. Have been highlighted the EMT stemness mechanism in Leukemic stem cell progression.
Keywords: the epithelial-to-mesencymal transition (EMT), EMT-inducer marker, N-cadherin, Snail, Twist, myeloid leukemias, leuklemic stem cell progression.
The determination of the stability of nitrogen-fixing microorganisms of the soil of Ecuador to toxic metals CrO42–, Ni2+, Cu2+
Authors: Bielikova O. Yu. , Matvieieva N. A. , Yastremskaya L. S. , Tashyrev A. B. 
Published: March 2020

Aim. The aim of the work was to determine the stability of nitrogen-fixing microorganisms isolated from the rhizosphere of bromelia (Ecuador), to the effect of toxic metals. Methods. Microorganisms were isolated on the Ashby nutrient medium. The selected strains were cultured on a medium with Cu2+ (Cu (ІІ) citrate) from 50 to 500 mg/l by cation in steps of 50; Ni2+ (NiCl2) from 20 to 200 mg/l by cation in steps of 20; Cr (VI) (K2CrO4) from 20 to 100 mg/l with Cr (VI) in increments of 20. The growth of microorganisms in the presence of metals was characterized by the maximum permissible concentration (MPC) of metals, duration of lag phase and the number of colony-forming units (CFU) of nitrogen-fixing microorganisms with increasing concentration of toxic metals. Results. The selected dominant nitrogen-fixing microorganisms from the soil of Ecuador were resistant to toxic metals (Cu2+, Ni2+, Cr (VI)) in high concentrations. It was found that MPC for microbial communities of nitrogen-fixing microorganisms were: 40 mg/l Cr (VI), 300 mg/l Cu2+ and 100 mg/l Ni2+. Conclusions. It was shown that the selected dominant nitrogen-fixing microorganisms from the Ecuadorian soil were resistant to toxic metals (Cu2+, Ni2+, Cr (VI)) in high concentrations, which in 4–30 times exceed the damage or bactericidal concentrations for the majority of known organophosphate microorganisms of natural ecosystems.
Keywords: metalresistance, nitrogen-fixing microorganisms, Cu2+, Ni2+, Cr (VI).
Use of DNA markers for studying the genetic polymorphism of the bread wheat (T. aestivum Desf.) genotypes of Azerbaijan
Authors: Hajiyev E. S. , Akparov Z. I. , Aliyev R. T. , Babayeva S. M. , Izzatullayeva V. I. , Abbasov M. A. , Mamedova A. D. 
Published: March 2020

Aim. The study was devoted to molecular-genetic evaluation of 110 bread wheat genotypes collected from different regions of Azerbaijan. Methods. Molecular-genetic. Results. The mean polymorphism level for the studied genotypes of bread wheat, using 8 ISSR markers, was 72 %. An index of genetic diversity calculated for the studied collection of T. aestivum specie was equal to 0.91. Among the bread wheat accessions, the highest genetic diversity was established for botanical variety var. erythrospermum (GDI=0.90). Conclusions. Primers UBC 112, UBC 841, UBC 857 and UBC 873 were identified to be more effective in identification of bread wheat accessions. As a result of the GGE biplot analysis, a trait collection was created by selecting 20 bread wheat samples with broad genetic variation. А core collection of bread wheat was created, which coversthe most distant genotypes and represents the genetic diversity of all 110 accessions studied.
Keywords: Triticum aestivum Desf., genetic polymorphism, ISSR markers.
Dependence of grain productivity of the main shoot ear on the stem dry matter weight in varieties of winter wheat
Authors: Zborivska O. V. , Priadkina G. O. , Sokolovska-Sergienko O. G. , Makharinska N. M. 
Published: March 2020

Aim. Estimation of the mass of dry matter of the stem of the main shoots of different varieties of winter wheat in the phases of flowering and full ripeness as a criterion for the grain productivity of its ear in years with different meteorological conditions. Methods. Field, morphometric, mathematical and statistical. Results. The mass of dry matter of the stem of the main shoots of winter wheat varieties was studied in years that differed significantly in weather conditions in the flowering phase and at full grain maturity. It was established that the mass of dry matter of the stem of the main shoot at the flowering and the full grain maturity varied significantly, respectively 0.6–1.2 g and 0.5–1.0 g in 2014, and 1.0–2.0 g and 0.8–1.5 g in 2016 depending on the variety, and under different growing conditions. Limits of variation of grain yield of the ear in 2014, ranged from 0.9 to 2.2 g and from 1.5 to 2.5 g in 2016. It was established that at the flowering, the correlation between the mass of grain from the head and the mass of the dry substance of the stem was significant at 99 % probability under growing conditions (r = 0.8–0.9). Conclusions. It is shown that grain productivity of main shoot in winter wheat is related to the ability of its stem to store assimilates during the vegetative period. It was established that the mass of the dry matter of the stem of the main shoots in the flowering phase can serve as a criterion for assessing its grain productivity.
Keywords: Triticum aestivum L., mass of stem, grain yield.
Biochemical analysis and assessment of modern monogamous varieties of hemp Cannabis sativa L. by the content of cannabinoid substances
Authors: Polyakova A. S. 
Published: March 2020

Aim. Defining the peculiarities of forming the cannabinoid substances in modern monogamous varieties of hemp is of both theoretical and practical importance. Methods. Modern monogamous varieties of hemp bred in Hlukhiv, Zolotonosha and specimens receive from France; were used for the chromatographically analysis of «damp» and dried in the shadow plant samples was conducted by the method of thin layer chromatography (TLC). Results. It was founding, that in the initial phase of growth and development the Ukrainian hemp varieties with the practical absence of neutral compounds and the French varieties with the sufficient content of these substances formed only natural acids. During the generative phase of growth and development, the varieties from France formed not only natural acids but also neutral compounds. The Ukrainian varieties demonstrated only insufficient increase of natural acids. Conclusions. In the initial phase of growth and development, the investigated hemp varieties containing both large and insignificant amount of cannabinoid substances formed only natural acids. Separate plants demonstrated the decrease of natural acids up to their total absence, which proves their biological interconnections and possible forming cannabidiol, tetrahydrocannabinol and сannabinol by way of fermentative decarboxylation the respective acids.
Keywords: Cannabinol (CBN), cannabidiol (CBD), tetrahydrocannabinol (THC), cannabidiolic acid (CBDA), tetrahydrocannabinolic acid (THCA).
Genetic diversity of spring common wheat varieties at storage protein loci
Authors: Sobko T. O. , Sirant L. V. , Lisova G. M. 
Published: March 2020

Aim. The aim of this study was to investigate genetic diversity of storage protein loci in spring common wheat varieties licensed for growing in Ukraine in 2017 year. Methods. SDS and APAG electrophoresis were used to identify genotypes at the gliadin loci Gli-1 and the high-molecular-weight glutenin subunit loci Glu-1. Results. Genotypes at the gliadin loci Gli A1, Gli B1, Gli D1 and glutenin loci Glu A1, Glu B1, Glu D1 were identified in 31 spring common wheat. The wheat-rye translocation AL/1RS was identified in two varieties Etyud and Struna mironivsyka. Differences in frequencies of alleles at storage protein loci were revealed. Conclusions. The high level of allelic variation was observed at the Gli-1 loci in spring common wheat varieties. Predominant alleles (one or two per locus) were revealed: Gli A1f, Gli A1а, Gli B1e, Gli D1b, Gli D1і, Gli D1f , Glu А1а, Glu Bс , Glu D1d, Glu D1a.
Keywords: Triticum aestivum L., spring wheat, storage protein.