bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frequency of HPV detection in chagasic megaesophagus associated or not with esophageal squamous cell carcinoma
Authors: Munari Fernanda Franco , Sichero Laura , Cruvinel-Carloni Adriana , Lacerda Croider Franco , Nunes Emily Montosa , Oliveira Antônio Talvane Torres de , Scapulatempo-Neto Cristovam , Silva Sandra Regina Morini da , Crema Eduardo , Adad Sheila Jorge , Rodrigues Maria Aparecida Marchesan , Henry Maria Aparecida Coelho Arruda , Guimarães Denise Peixoto , Reis Rui Manuel , Villa Luisa Lina , Longatto-Filho Adhemar 
Published: July 2020

Abstract
Background: Chagasic megaesophagus (clinical manifestation of chagasic disease) has been reported as an etiological factor for squamous cell carcinoma of the esophagus, as well as the presence of human papillomavirus (HPV). Objective: We accessed the prevalence of HPV DNA in a series of squamous cell carcinomas of the esophagus associated or not with the chagasic megaesophagus, and within samples of chagasic megaesophagus without cancer. Data obtained was further correlated to the pathological clinical data of affected individuals. Methods: Retrospective study that used a total 92 samples tissue/biopsy specimens of formalin fixed and paraffin embedded tissues were retrospectively collected from the southeast region of Brazil from patients treated in three hospitals: Barretos Cancer Hospital, Barretos, São Paulo; Federal University of Triângulo Mineiro (UFTM), Uberaba, Minas Gerais; and São Paulo State University (UNESP), Botucatu, São Paulo. Cases were divided in three groups: i) 24 patients with chagasic megaesophagus associated with esophageal ESCC (CM/ESCC); ii) 37 patients with esophageal ESCC without chagasic megaesophagus (ESCC); iii) 31 patients with chagasic megaesophagus without esophageal ESCC (CM). Results: We detected a higher prevalence of high-risk HPVs in patients from both CM (12/31, 38.8%) and CM/ESCC groups (8/24, 33.3%), as compared to individuals of the ESCC group (6/37, 16.3%), although data was not statistically significant. We further observed that HPV-16 was more prevalent in patients of the ESCC (4/9, 44.5%) and CM/ESCC groups (2/8, 25.0%). In addition, some of these samples presented infection by multiple HPV types. High-risk HPVs detected were HPV-31, 45, 51, 53, 56, 66, and 73, of which the majority was identified in patients from the CM group. Furthermore, low-risk HPV-11 and HPV70 were identified in individuals from both ESCC and CM groups. Conclusion: This is the first report regarding the presence of HPV DNA in megaesophagus associated with esophageal squamous cell carcinoma. In the present study, HPV infection appears to be directly related to the development of esophageal squamous cell carcinoma in patients with chagasic megaesophagus. Further studies are warrantee to confirm and better understand the role of oncogenic HPV persistent infection in these patients.
Detecting the optimal condition of Zeolite utilization in industrial wastewater treatment by using the fuzzy simulation model
Authors: Abolpour Behrouz , Sheibani Sahar , Eskandari Amir 
Published: July 2020

Abstract
To define optimal condition of Zeolite utilization was used the maximum industrial pollution load from the industrial wastewater discharged to treatment plant lied on the industrial town of Shiraz, Fars, Iran. In this study, chemical oxygen demand (COD) load and electrical conductivity (EC) load from this wastewater treatment plant (WWTP) along with the parameters of received industrial effluents, e.g., temperature, total suspended solid (TSS), total dissolved solid (TDS) and PH were monitored. Using the several mathematical models that defined on their relationships was resulted the correlation coefficients of 83% and 90% for COD with TDS, and COD with TSS, respectively, thus the best regression coefficient was 0.5 under linear and nonlinear forms. Autoregressive integrated moving average model (ARIMA) was also used for obtaining the better results, such that the exceeded values of TSS and TDS at before day were defined as input variables. In this log-time of bioreactor process, curve fitting approach and clustering analysis with and without normalized data had not improved the regression coefficient of linear and nonlinear functions. The simulation model based on fuzzy inference system (FIS) has been good corresponding with the distribution of estimated and observed data of COD, so that comparing such distribution of COD with line of 1:1 resulting the regression coefficient is 0.764. This study indicated that fixed value of the soluble solids concentration on industrial wastewater discharged into treatment plant has the important role in effectiveness of Zeolite filtration, i.e., it's threshold is occurred in 1746 ppm in this case study.
Genome Sequences of Brucella Melitensis Strains QH2019001 and QH2019005 Provides Insight Into Different Evolutionary Origins Within Qinghai Province, China
Authors: Zhao Zhijun , Li Jiquan , Ma Li , Xue Hongmei , Yang Xuxin , Zhao Yuanbo , Qin Yumin , Yang Xiaowen , Piao Dongri , Zhao Hongyan , Tian Guozhong , Li Qiang , Tian Guang , Wang Jianling , Jiang Hai , Xu Liqing 
Published: July 2020

Abstract
Background

Brucellosis, which is cause by Brucella, is one of the most important zoonoses and is considered a “forgotten, neglected zoonosis” by the WHO.
Methods

Blood samples were isolated from two brucellosis males that were confirmed B. melitensis positive following multiple loci variable-number tandem repeat analysis (MLVA) and strains QH2019001 and QH2019005 were identified. Genomic DNA was extracted from these samples and whole genome sequencing (WGS) was performed. Next, SNP-based phylogenetic analysis was performed to compare the two strains to 94 B. melitensis strains (complete genome and draft genome) retrieved from online databases.
Results

The QH2019001 and QH2019005 strains were found to have differences in their variable-number tandem repeats. Phylogenetic examination of the 96 strains assigned them to 5 genotype groups, with QH2019001 and QH2019005 assigned to the same group, but to different subgroups; with the QH2019005 strain assigned to a new subgenotype, IIj. These findings were then combined to determine strain origin.
Conclusions

Utilizing a whole genome SNP-based approach highlights the diversity between B. melitensis strains, and it can facilitate the elucidation of different evolutionary histories. This approach also revealed that the QH2019005 is part of a new subgenotype with an ancient origin in the eastern Mediterranean Sea.
Human atlas of cardiac promoters and enhancers reveals important role of regulatory elements in heritable diseases
Authors: Deviatiiarov Ruslan , Gams Anna , Syunyaev Roman , Tatarinova Tatiana , Gusev Oleg , Efimov Igor 
Published: July 2020

Abstract
Genome regulatory elements play a critical role during cardiac development and maintenance of normal physiological homeostasis, and genome-wide association studies identified a large number of SNPs associated with cardiovascular diseases localized in intergenic zones. We used cap analysis of gene expression (CAGE) to identify transcription start sites (TSS) with one nucleotide resolution that effectively maps genome regulatory elements in a representative collection of human heart tissues. Here we present a comprehensive and fully annotated CAGE atlas of human promoters and enhancers from four chambers of the non-diseased human donor hearts, including both atria and ventricles. We have identified 10,528 novel regulatory elements, where 2,750 are classified as TSS and 4,258 novel enhancers, which were validated with ChIP-seq libraries and motif enrichment analysis. We found that heart-region specific expression patterns are primarily based on the alternative promoter and specific enhancer activity. Our study significantly increased evidence of the association of regulatory elements-located variants with heart morphology and pathologies. The precise location of cardiac disease-related SNPs within the regulatory regions and their correlation with a specific cell type offers a new understanding of genetic heart diseases.
Acupuncture as a complementary therapy for cancer care: acceptability and preferences of patients and informal caregivers
Authors: Lefebvre Tessa , Tack Laura , Blieck Virginie , Cool Lieselot , Pottel Hans , Eygen Koen Van , Derijcke Sofie , Vergauwe Philippe , Schofield Patricia , Chandler Rebecca , Lane Pauline , Boterberg Tom , Debruyne Philip 
Published: July 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Dynamic Alterations on Synthesis and Transportation of Vitamin C and Deposition Status in Produced Eggs Induced by Dietary Vitamin C Supplementation in Hy-Line Brown Layer Model
Authors: Zhu Yufei , Zhao Jianfei , Guo Wei , Qin Kailong , Yan Jiakun , Huang Xinhuo , Ren Zhouzheng , Yang Xin , Yang Xiaojun 
Published: July 2020

Abstract
Background: Some previous studies have indicated that in ovo feeding (IOF) of vitamin C (VC) had positive effects on the performance in poultry. In order to realize embryonic VC supplementation, an idea about hen’s dietary VC supplementation to achieve VC enrichment in produced eggs was proposed. And this study was executed to investigate the effects of dietary VC supplementation on synthesis and transportation of VC in layers and VC deposition status in produced eggs.Results: Compared with Arbor Acres breeder eggs, egg VC content was lower in Isa Brown breeder eggs and Hy-Line Brown layer eggs (P < 0.05). Sodium-dependent vitamin C transporter 1 (SVCT1) and SVCT2 expression was higher in ileum than in duodenum and jejunum (P < 0.05). SVCT1 expression was extremely higher in magnum than in ovary, while SVCT2 expression was lower (P < 0.05). L-gulonolactone oxidase (GLO) expression was extremely higher and SVCT1 expression was higher in kidney than in liver, while SVCT2 was lower (P < 0.05). 400 mg/kg VC supplementation increased SVCT1 expression in duodenum, ovary and magnum, while decreased GLO and SVCT1 expression in liver (P < 0.05). 200 and 400 mg/kg VC supplementation increased SVCT2 expression in duodenum, while decreased GLO and SVCT1 expression in kidney and SVCT2 expression in liver (P < 0.05).Conclusions: Hy-Line Brown layer was a useful model for investigating effects of dietary VC supplementation on VC deposition in produced eggs. Dietary VC supplementation promoted VC absorption in duodenum and jejunum, but reduced endogenous VC synthesis in liver and kidney. Although dietary VC supplementation enhanced VC transportation in ovary and magnum, it finally failed to increase VC deposition in produced eggs.
A Cross-sectional Study: Association Between sarcopenia and Osteoporosis in Type 2 Diabetes Mellitus Patients With a High Glycated Hemoglobin Level
Authors: Lingna Fang , Zhong Shao , Ma Dan , Lu Bing , Shen Liwen , Zhang Li , Tang Fengyan , Sun Heping 
Published: July 2020

Abstract
Background: Sarcopenia and osteoporosisare commonly observed in individuals with type 2 diabetes mellitus (T2DM).Thus, this study investigated the association between sarcopenia and osteoporosis in T2DM patients with a highglycated hemoglobin (HbA1c)level.Methods: This study recruited 98 Chinese patients with T2DM who were aged ≥ 50 years, and their body compositions were evaluated using dual-energy X-ray absorptiometry. Moreover, the fFasting blood glucose, HbA1c, B collagen-specific sequences (B-CTX), osteocalcin(OC), propeptide of type 1 procollagen(P1NP), 25-hydroxy vitamin Dlevels were evaluated.Sarcopenia was defined as skeletal muscle mass index (SMI) <7.0 kg/m2 for men and < 5.4 kg/m2 for women.Results: There were 42 men and 56 women, with an average age of 65.86 ± 8.32 years and HbA1c level of 8.59% ± 1.87%. The prevalence rates ofsarcopenia, osteopenia, and osteoporosis were48.0%, 37.8%, and 33.6%, respectively.Moreover, the prevalence of osteoporosis was significantly higher in women than in men (50% vs. 11.9%, P = 0.000).The prevalence of sarcopenia was significantly higher in patients with HbA1c levels> 9.0% than in those with HbA1c levels < 9.0% (62.2% vs. 39.3%, P = 0.037). However, the prevalence of osteopenia and osteoporosis in patients with HbA1c levels > 9.0% differed from those with HbA1c levels < 9.0%(29.5% vs.51.4% for osteopenia, 32.8% vs.35.1% for osteoporosis; P = 0.022). The height and weight of the osteoporosis group significantly decreased compared with those of the normal bone mineral density (BMD), whereas the serum BCTX, OC, and PINP levels significantly increased.The SMI, trunk muscles, skeletal muscle parameters, lumbar spine bone mineral content (BMC), lumbar spine BMD, femoral BMC, and femoral BMD decreased significantly in T2DM patients with osteoporosis.Sarcopeniawas associated with a higher height (odds ratio [OR] = 1.524, 95% confidence interval [CI] = 1.196-1.698, P = 0.000), lower body weight (OR = 0.735, 95% CI = 0.640-0.844, P = 0.000), and higher body fat content (OR = 1.211, 95% CI = 1.046-1.402, P = 0.011). Meanwhile, osteoporosis was correlated to a lower SMI (OR = 0.178, 95% CI = 0.044-0.817, P = 0.015).Conclusions: Ahigh HbA1c level was associated with a higher prevalence of sarcopenia and osteoporosis in T2DM patients, and low muscle masswas considered a risk factor for osteoporosis inthis group of patients.
Ellagic Acid Attenuates BLM-Induced Pulmonary Fibrosis via Inhibiting Wnt Signaling Pathway
Authors: Li Xiaohe , huang kai , Liu Xiaowei , Ruan Hao , Ma Ling , Cui Yunyao , Wang Yanhua , Wu Shuyang , Li Hailong , Wei Yuli , Liang Jingjing , Li Zeping , Gao Jingjing , Yang Bo , Li Xiaoping , Zhou Honggang , Yang Cheng 
Published: July 2020

Abstract
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with high mortality, which characterized by epithelial cell damage and fibroblasts activation. Ellagic acid (EA) is a natural polyphenol compound widely found in fruits and nuts which has demonstrated multiple pharmacological activities. Herein we showed that Ellagic acid significantly alleviated bleomycin(BLM)-induced pulmonary fibrosis in mice, and also inhibited the Wnt/β-catenin signal in primary pulmonary fibroblasts. In vitro experiments indicated that Ellagic acid apparently suppressed Wnt3a-induced myofibroblasts activation and ECM accumulation mainly via inhibiting the phosphorylation of Erk2 and Akt. Further studies showed that Ellagic acid could induce autophagy formation of myofibroblasts mainly by suppressing mTOR signaling and promote apoptosis of myofibroblasts. In vivo experiments reveled that Ellagic acid significantly inhibited myofibroblasts activation and promoted autophagy formation. Taken together, our results showed that Ellagic acid effectively attenuated BLM-induced pulmonary fibrosis in mice by suppressing myofibroblasts activation, promoting autophagy and apoptosis of myofibroblasts mainly via inhibiting Wnt signaling pathway.
Interannual and Intra-seasonal Oscillations in the Length of Day, Atmospheric Angular Momentum and Atmospheric Surface Pressure Time Series Observed at Chosen Meteorological Stations Near the Equator
Authors: Ma Lihua , Kosek Wieslaw , Han Yanben 
Published: July 2020

Abstract
The atmospheric surface pressure time series of Madras, Darwin, and Tahiti together with non-tidal length-of-day (LODR) variations and axial component of atmospheric angular momentum (AAM) were analyzed by wavelet transform as well as the combination of the Fourier transform band pass filter with the Hilbert transform (FTBPF + HT) to detect interannual and intra-seasonal oscillations in them. It was found that annual oscillations in the atmospheric surface pressure variations of Darwin and Tahiti stations are in phase and are about 180o out of phase in the atmospheric surface pressure variations of Madras station. The phase of the annual oscillation in atmospheric surface pressure variations of Madras station is slightly greater (~ 20o) than the phase of the annual oscillation in the LODR time series. The amplitude and phase variations of the annual and semi-annual oscillations computed by the FTBPF + HT combination in LODR and the axial component of AAM are very similar. The mean amplitudes of the semi-annual oscillation in the atmospheric surface pressure variations of Madras and Tahiti are of the order of 0.4 hPa, the phases of these oscillations are stable and the amplitude of the semi-annual oscillation in the atmospheric surface pressure variations of Darwin is negligible due to unstable phase of this oscillation. The atmospheric surface pressure variations of Madras, Darwin, and Tahiti stations show similar amplitude wideband signals with a central period of ~ 4 years (cutoff periods ranging from about 2.2 to 20 years) related to El Niño phenomenon. The amplitude maxima of this signal corresponding to the strongest El Niño events in 1982-83, 1997-98, and 2014-15 are also present in amplitude variations of this signal in the LODR and AAM χ3 time series.
The identification of a potential prognostic model of colon cancer using integrated bioinformatics analysis
Authors: Fang Zhengyu , Xu Sumei , Xie Yiwen , Yan Wenxi 
Published: July 2020

Abstract
Background

This study aimed to construct prognostic model by screening prognostic gene signature of colon cancer.
Methods

The gene expression profile data of colon cancer were obtained from The Cancer Genome Atlas (TCGA) and gene expression omnibus (GEO) and differently expressed genes (DEGs) between tumor and control samples were identified. Prognosis-associated genes were then identified and used for the construction of prognostic model. The independent factors that associated with the prognosis of colon in the TCGA cohort was identified.
Results

Totally, 1153 consistent DEGs were screened out between tumor and normal tissues in the TCGA cohort, GSE44861 and GSE44076 datasets. Among these genes, 12 DEGs were related to the prognosis of colon cancer and were used for constructing the prognostic model. This model presented a high predictive power for the prognosis of colon cancer both in the training dataset and in the validation datasets (AUC > 0.8). Statistical analysis showed that age, pathological T, tumor recurrence, and model status were the independent factors for prognosis of patients with colon cancer in TCGA.
Conclusions

The 12-gene signature prognostic model had a high predictive power for colon cancer prognosis.
CARE: An Observational Study of Adherence to Home Nebulizer Therapy Among Children with Asthma
Authors: Zhao Deyu , Chen Dehui , Li Ling , Zou YingXue , Shang Yunxiao , Zhang Chonglin , Zhang Li , Pan Jiahua , Chen Qiang , Ai Tao , Ni Qian 
Published: July 2020

Abstract
Background The prevalence of pediatric asthma in China is approximately 3%, and asthma remains poorly controlled in many of these patients. This study assessed the rate of adherence to home nebulizer treatment in pediatric patients in China.Methods The CARE study was a 12-week, multicentre, prospective, observational study across 12 tertiary hospitals in China. Patients were aged 0–14 years, clinically diagnosed with asthma and prescribed home nebulizer inhaled corticosteroid (ICS) therapy for ³ 3 months. The primary endpoint was electronically monitored treatment adherence. Patients attended onsite visits at 0, 4, 8 and 12 weeks to assess asthma control, severity and treatment adherence (recorded by electronic monitoring devices and caregivers).Results The full analysis set included 510 patients. Median treatment adherence reported by electronic monitoring devices was 69.9%, and median caregiver-reported adherence was 77.9%. The proportion of patients with well-controlled asthma increased from 12.0% at baseline to 77.5% at Visit 4. Increased time between asthma diagnosis and study enrolment was a significant predictor for better adherence (coefficient: 0.01 [p = 0.0138, 95% confidence interval: 0.00, 0.01]) and asthma control (odds ratio = 1.001, p = 0.0498, 95% CI: 1.000, 1.002). Negative attitude to treatment by the caregiver was associated with poorer asthma control.ConclusionsAdherence to home nebulization, a widely-used treatment for asthma, was high among Chinese pediatric patients. Asthma control improved with increasing treatment duration. These results suggest that home nebulization of ICS is an effective and recommendable long-term treatment for pediatric patients with asthma. Trial registrationClinicalTrials.gov, NCT03156998.
Prevalence of Intestinal Protozoa Infections and Associated Risk Factors Among Preschool Aged Children in Mkuranga District, Tanzania: A Community Based Cross-Sectional Study
Authors: Mustafa Ummul-Khair , Zacharia Abdallah , Mushi Vivian 
Published: July 2020

Abstract
Background: Intestinal protozoa infections are among the serous public health problems in preschool aged children (PSAC) and can lead to considerable health problems such as growth retardation, anemia, nutrition deficiency, physical weakness and mental health problems. In Tanzania, there is a paucity of data on intestinal protozoa infections though the evidence shows that PSAC can be infected. Therefore, this study aimed to determine the prevalence of intestinal protozoa infections among PSAC and their associated risk factors in Mkuranga district, Tanzania.Methodology: A community based cross-sectional study was conducted between April and June 2019 to determine the prevalence of intestinal protozoa. A total of 525 stool samples were collected from PSAC and processed using formal-ether concentration method. Risk factors were identified using questionnaire interview conducted among parents/guardians. All participants were randomly selected from two villages (one located in rural and another in suburban settings). Data were entered and analysed by using SPSS version 23.Results: A total of 20 (3.8%) out of 525 PSAC were infected with intestinal protozoa (Entamoeba coli or Giardia lamblia). Among the assessed risk factors the following were significantly associated with intestinal protozoa: age of PSAC (AOR = 1.05, p = 0.048), age group of parents/guardians (AOR 30-39 years/50 -59years = 0.16, p = 0.031), household population (AOR 1-5/11+ = 0.15, P = 0.018 and AOR 6-10/11+ = 0.10, P = 0.010) and source of water ((AOR tape within household/spring = 0.004, p = 0.015), (AOR protected well/spring = 0.006, p = 0.023), (AOR bore hole/spring/spring = 0.006, p = 0.012), (AOR tape away of home/spring = 0.009, p = 0.020) and (ARO tape at home/spring = 0.004, p = 0.015)).Conclusion: There was a low prevalence of intestinal protozoa infection among PSAC in the two communities studied. Despite low prevalence of intestinal protozoa, the transmission is present hence the need to initiate control measures. Health education should be provided to parents/guardians of PSAC on protecting water sources, treatment of drinking water and supervision of latrine usage by PSAC and introduction of hand washing facilities are crucial.