bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parthenolide inhibits the growth of non-small cell lung cancer by targeting epidermal growth factor receptor
Authors: Li Xiaoling , Huang Riming , Li Mingyue , Zhu Zheng , Chen Zhiyan , Cui Liao , Luo Hui , Luo Lianxiang 
Published: July 2020

Abstract
Background: EGFR tyrosine kinase inhibitors (TKIs) have been developed for the treatment of EGFR mutated NSCLC. Parthenolide, a natural product of parthenolide, which belongs to the sesquiterpene lactone family and has a variety of biological and therapeutic activities, including anti-cancer effects. However, its effect on non-small cell lung cancer is little known. Methods: The CCK-8 assay and colony formation assays were used to assess cell viability. Flow cytometry was used to measure the cell apoptosis. In silico molecular docking was used to evaluate the binding of parthenolide to EGFR. Network pharmacology analysis was was used to evaluate the key gene of parthenolide target NSCLC. Western blotting was used to evaluate the key proteins involved apoptosis and EGFR signalling. The effect of parthenolide treatment in vivo was determined by using a xenograft mouse model. Results: In this study, parthenolide could induce apoptosis and growth inhibition in the EGFR mutated lung cancer cells. Parthenolide also reduces the phosphorylation of EGFR as well as its downstream signaling pathways MAPK/ERK and PI3K/Akt. Molecular docking analysis of EGFR binding site with parthenolide show that the anti-cancer effect of parthenolide against NSCLC is mediated by a strong binding to EGFR. Network pharmacology analysis show parthenolide suppresses NSCLC via inhibition of EGFR expression. In addition, parthenolide inhibits the growth of H1975 xenografts in nude mice, which is associated with the inhibition of the EGFR signaling pathway. Conclusions: Taken together, these results demonstrate effective inhibition of parthenolide in NSCLC cell growth by targeting EGFR through downregulation of ERK and AKT expression, which could be promisingly used for patients carrying the EGFR mutation.
A modular multi-agent framework for innovation diffusion in changing business environments: conceptualization, formalization and implementation
Authors: Johanning Simon , Scheller Fabian , Abitz Daniel , Wehner Claudius , Bruckner Thomas 
Published: July 2020

Abstract
Understanding how innovations are accepted in a dynamic and complex market environment is a crucial factor for competitive advantage. To understand the relevant factors for this diffusion and to predict success, empirically grounded agent-based models have become increasingly popular in recent years. Despite the popularity of these innovation diffusion models, no common framework that integrates their diversity exists. This article presents a flexible, modular and extensible common description and implementation framework that allows to depict the large variety of model components found in existing models. The framework aims to provide a theoretically grounded description and implementation framework for empirically grounded agent-based models of innovation diffusion. It identifies 30 component requirements to conceptualize an integrated formal framework description. Based on this formal description, a java-based implementation allowing for flexible configuration of existing and future models of innovation diffusion is developed. As a variable decision support tool in decision-making processes on the adoption of innovations the framework is valuable for the investigation of a range of research questions on innovation diffusion, business model evaluation and infrastructure transformation.
Sequential use of hemadsorption using Cytosorb® and Biosky® filter- technology in a COVID-19 patient suffering from severe ARDS
Authors: Mezger Matthias , Eitel Ingo , Ensminger Stephan , Pogorzalek Dirk , Huang Zhipan , Graf Tobias 
Published: July 2020

Abstract
In March 2020, the World Health Organization (WHO) declared the novel coronavirus disease (COVID- 19) pandemic. Here, we present the case of a patient who was admitted to our hospital with acute respiratory distress syndrome (ARDS) following infection with COVID-19. After initial stabilization through restrictive fluid management, hemadsorption using Cytosorb® was performed and finally temporary extubation of the patient was possible. However, the patient again clinically deteriorated and needed ventilation and finally ECMO-support and high catecholamine application. Whilst being on VV- ECMO, hemadsorption using Biosky® MG 350 filter was performed. In this manuscript, after a brief overview of the role of hemadsorption in ARDS, a detailed case presentation is followed by a critical discussion of the current literature.
Early Recurrent Dislocation and Dissociation of Bipolar Cup: Two Intricate Cases and Review of Literature.
Authors: Fan Xiao-lei , Wang Jian , Zhang De-hua , Mao Feng , Liao Yi 
Published: July 2020

Abstract
Background: Bipolar hemiarthroplasty (BHA) is one of the best options to treat femoral neck fracture in elderly patients. However, the early dislocation problem also happens in the bipolar cup. Furthermore, BHA has a specific implant-related complication, namely bipolar cup dissociation, in which the femoral head dissociates from the polyethylene (PE) liner. Early dislocation and dissociation are relatively uncommon but devastating complications and almost always lead to reoperation with revision of the prosthesis. Dislocation and dissociation of the bipolar cup can be challenging to manage and easily turn to recurrent episodes in case of improper handling. We hope to find out the best strategy for treatment early recurrent dislocation and dissociation of the bipolar cup.Methods: We retrospectively reviewed 2 patients who had a history of early recurrent posterior dislocations following primary BHA from 2016 to 2019. What’s more, they had early recurrent bipolar cup dissociation during the attempt of closed reduction maneuvers within 3 months of the original surgery.Results: All the patients were treated with open reduction and revision of Bipolar hemiarthroplasty (BHA). One patient has a good outcome, and no recurrence of dislocation was recorded.Conclusion: Recognition of the risk of dislocation and dissociation of bipolar cup is essential to its prevention and treatment. Complete revision of bipolar parts and the femoral head may be the minimum solution necessary to avoid the early recurrence of bipolar cup dissociation. Further investigations are recommended to strengthen these results.
Is COVID-19 a Risk Factor of DVT in Orthopaedic Trauma Surgery? A Prospective Study
Authors: Agar Anıl , Gunes Orhan , Sahin Adem , Kılıc Bulent , Dogan Sumeyra , Kocatas Ali , Erturk Cemil , Gulabi Deniz 
Published: July 2020

Abstract
Objective: The aim of this prospective study was to evaluate the radiological and laboratory parameters of patients diagnosed with COVID-19 who underwent surgery for a lower extremity fracture in our COVID-19 care units in terms of DVT.Patients and Methods: Patients who were operated on due to lower extremity fracture between 10 March and 1 May and diagnosed with COVID-19 were evaluated prospectively. Patients’ age, gender, affected side, fracture location, fracture type, COVID-19 radiological or clinical symptoms and Doppler USG and biochemical markers screening for DVT were evaluated.Results: Thirty patients, ages 39-88, were included in the study. Preoperative D-dimer value of the patients was mean 5.9 mg / L. In all the patients, the D-dimer level was above the normal range. The mean troponin value was 0.025 ng / mL preoperatively. The troponin value was found to be normal in 8 patients and above the normal value in 22 patients. On physical examination, DVT findings were present in 1 patient and DVT was detected in 2 patients on doppler ultrasound.Conclusion: It can be recommended that extra attention should be given to vascular complications in COVID-19 positive trauma patients, as both the effect of trauma itself increases hypercoagulability and COVID-19 disease seems to have the potential to increase hypercoagulability.
Prevalence of Bedbugs Infestation, Crowding and Awareness Level Among Government Boarding Secondary School Students in Moshi Urban, Tanzania
Authors: Massawe Peter Raphael , Wahhay Ezekiel Emmanuel , Gideon Baraka , KITAU JOVIN , Mushi Amedeus 
Published: July 2020

Abstract
Background. Bedbug infestation is the worldwide problem according to World Health Organization, and the infestation is characterized by biting reactions including systemic, cutaneous reactions and sometimes psychological disturbance. Prevalence of bedbug infestation is low (6%) in developed countries while in Sierra Leone is 98% and Tanzania is 56% in which is tremendously high. As the bedbug infestation is among neglected conditions and because of high prevalence in Tanzania, the studies to determine this burden, some risk factors (crowding) and awareness among specific populated communities especially schools is inevitable for planning strategies in eradication of bedbug.Objective. To determine the prevalence of bedbug infestation, crowding and awareness level among boarding government secondary school students in Moshi Urban.Methodology. A cross-sectional study was conducted from May to July 2018 at Moshi Urban in Kilimanjaro region. The structured standard questionnaire was used to obtain the demographics information of participants, asses awareness. Personal observation was used to determine prevalence and crowding in the corresponding student’s dormitories. Results. The prevalence of bedbug infestation was 58.1% (223) among 384 study participants. Logistic regression analysis showed that 72.6% of the students with bedbug infestation were found to be living in the overcrowded dormitories. There was association between overcrowd and bedbug infestation (OR=2.50, 95%CI (1.63-3.83). Only 32.7% of the infested students involved in this study were aware with bedbugs (OR=0.8795% CI (0.56-1.35) P=0.54. Conclusion. The overall prevalence of bedbug infestation was found to be high among students living in the overcrowded dormitories and those who were not aware on bedbug biology and behavior, transmission, control and prevention ways. Education concerning bedbugs is needed to increase the community awareness together with management of number of student in accordance to the available facilities and resources.
PPARα and PPARβ/δ Expression is Associated with Proinflammatory Markers in an Obese Pediatric Population
Authors: Vargas-Sánchez Karina , Vargas Laura , Urrutia Yenny , Beltrán Iván , Rossi Ana Beatriz , Lozano Hernán Yupanqui , Guarín Jorge , Losada-Barragán Monica 
Published: July 2020

Abstract
Background
Obesity configures a pathophysiological profile that predisposes the development of metabolic and cardiovascular diseases, critically impacting public health. The chronic dysregulation of immuno-metabolic components triggered by obesity directly affects the metabolism of various tissues. Peroxisome proliferator-activated receptors (PPARs) are a group of nuclear receptors and transcription factors essential for energy homeostasis of different tissues. Despite its wide-ranging expression and function, the functional mismatch of the PPAR subtypes establish biological mechanisms responsible for metabolic diseases from childhood. In addition, the glucagon-like peptide-1 receptor (GLP-1R) activation can influence insulin secretion, but also the cytokine profile possibly mediated through PPARγ activation. However, to date, the role of PPARs and GLP-1R in leukocytes from obese pediatric patients has been unclear. Therefore, we examined the expression of PPARs isotypes and GLP-1R, and its correlation with metabolic, hormonal, inflammatory, and anthropometric markers in an obese pediatric population.
Results
Obese children and adolescents presented a significant increase in anthropometric and body composition parameters, TG, VLDL, TG/HDL, A/G index, and HOMA score when compared to the control group. Obese participants exhibited a pro-inflammatory profile with an augment of IL-8 (p = 0,0081), IL-6 (p = 0,0005), TNF-α (p = 0,0004), IFN-γ (p = 0,0110), MCP-1 (p = 0,0452), and adipsin (p = 0,0397), whereas displayed a reduction of adiponectin (p = 0,0452). The expression of PPARα and GLP-1R was lower in the leukocytes from obese participants than in lean subjects. Furthermore, PPARα correlates negatively with TNF-α (p = 0,0383), while GLP-1R did not show correlation with any inflammatory variable. However, both receptors correlate negatively with the abdominal skinfold. Although PPARβ/δ expression was similar between groups, it was negatively associated with IL-8 levels (p = 0,0085).
Conclusions
PPARα and PPARβ/δ expression are negatively associated with the proinflammatory markers TNF-α and IL-8, respectively, suggesting participation in the regulation of inflammation observed in pediatric obesity. Furthermore, PPARα and GLP-1R are downregulated in leukocytes from obese children and adolescents, and both receptors are associated with the abdominal skinfold suggesting a role in fat distribution that could indirectly affect the function of different immune cells and the secreted cytokines. Thereby, these findings may impact the understanding and implementation of PPARα or GLP-1R agonists in the clinic.
Circ0120816 Acts As An Oncogene of Esophageal Squamous Cell Carcinoma via Inhibiting miR-1305 and Releasing TXNRD1
Authors: Li Xiaoyong , Song Laichun , Wang Bo , Tao Chao , Shi Lei , Xu Ming 
Published: July 2020

Abstract
Background: The morbidity and mortality of esophageal squamous cell carcinoma (ESCC) remains stubbornly high, in spite of emerging new diagnoses methods. The role of circular RNAs (circRNAs) in ESCC progression is still in need of more exploration. Methods: In this research, we gathered 36 patients’ ESCC tissues to analyze the expression of circ0120816, miR-1305 and TXNRD1. KYSE450 and KYSE510 cells were used to conduct the transfection. Aiming to verify our hypothesis, qRT-PCR, RNase R treatment, nuclear extraction, luciferase reporter assay, RNA immunoprecipitation assay, CCK-8, BrdU, cell adhesion, caspase 3 activity assay were used. Results: Circ0120816, upregulated in ESCC, acted as a sponge for miR-1305. Circ0120816 combined miR-1305 to enhance cell viability, proliferation adhesion and repress cell apoptosis in ESCC cell lines. On the contrary, miR-1305 exerted reversed effect in ESCC cells through decreasing TXNRD1. Conclusions: This research demonstrated that circ0120816 promoted ESCC development by competitive binding miR-1305 which could increase the expression of TXNRD1.
hsa_circ_0000231 Promotes Colorectal Cancer Cell Growth Through Upregulation of CCND2 by IGF2BP3/miR-375 Dual Pathway
Authors: Zhang Wei , Wang Bo , Yang Yang , Zhang Zhen , Wang Quan , Zhang Haoran , Jiang Kewei , Ye Yingjiang , Wang Shan , Shen Zhanlong 
Published: July 2020

Abstract
Background
circular RNAs (circRNAs) recently have been emerged as vital regulators for involvement of initiation and progression of diverse kinds of human cancers. This study aimed to investigate the role of circRNAs in colorectal cancer (CRC).
Methods
The expression profile of circRNAs in 5 pairs of CRC tissues and adjacent normal tissues were analyzed by Microarray. Quantitative real-time PCR and in situ hybridization and Base Scope Assay were used to determine the level and prognostic values of hsa_circ_0000231. Then, functional experiments in vitro and in vivo were performed to investigate the effects of hsa_circ_0000231 on cell proliferation. Mechanistically, fluorescent in situ hybridization, dual luciferase reporter assay, RNA pull-down and RNA immunoprecipitation experiments were performed to confirm the interaction between hsa_circ_0000231 and IGF2BP3 or has_miR-375.
Results
hsa_circ_0000231 was evidently up-regulated in CRC primary tissues, which was indicated to poor prognosis of CRC patients. The results demonstrated that hsa_circ_0000231 could promote CRC cell proliferation as well as tumorigenesis in vitro and in vivo. Mechanistic analysis showed that hsa_circ_0000231 might on the one hand act as a ceRNA (competing endogenous RNA) of miR-375 to regulate cyclin D2 (CCND2), and on the other hand bind to IGF2BP3 protein to protect CCND2 from being degraded.
Conclusion
Our findings suggest that hsa_circ_0000231 facilitated CRC progression by sponging miR-375 or binding to IGF2BP3 to modulate CCND2. This discovery implied that has_circ_0000231 may be a potential new diagnostic and therapeutic biomarker for CRC.
Plant Nutrient Quality Promotes Survival and Reproductive Fitness of the Dengue Vector Aedes Aegypti
Authors: Nyasembe Vincent Odhiambo , Tchouassi David P. , Muturi Martha N. , Pirk Christian W. W. , Sole Catherine L. , Torto Baldwyn 
Published: July 2020

Abstract
Background: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae) on survival, fecundity, and egg viability of the dengue vector Aedes aegypti. Methods: We tested these effects using females that received a) an initial three rations of bloodmeal, and b) received no bloodmeal at all. Two controls were included; age-matched females fed on glucose solution with or without initial bloodmeal, and those fed exclusively on bloodmeal. Data was collected daily over a 30-day period. The amino acid content of Ae. aegypti guts and the amino acid content of their respective diets was detected by coupled liquid chromatography – mass spectrometry.Results: Females fed on P. dulce and exclusive bloodmeal had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on bloodmeal, whereas those fed on O. ficus-indica had the shortest survival time. With initial bloodmeal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs, compared to those fed exclusively on bloodmeal. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. Conclusion: Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors which may impact their disease transmission potential.
Quality of Road Trauma Data in Malawi: The Case of Road Trauma Surveillance in Ntcheu District, Malawi.
Authors: Manyozo Steven Derrick , Sichembe Wantula , Moodie Rob 
Published: July 2020

Abstract
Background Road trauma represents a major but neglected public health challenge in Malawi that requires concerted efforts for effective and sustainable prevention. In order to make the road traffic system safer, it is important to develop an understanding of the whole system and its elements including vehicles, roads and road-users along with their physical, social and environmental circumstances. In this study, we measured data quality in terms of database and variable completeness of road trauma case reports to determine if the data are sufficient for informing, monitoring and evaluating road safety interventions. Methods This was a quantitative retrospective study based in Ntcheu district, Malawi. Data were collected for year 2018 from both police and hospital sources. Categorical data exploration from these two sources was done using frequency distribution tables. Continuous data were summarized using means and standard deviation. Data source completeness was assessed using the capture–recapture methodology, while variable completeness was assessed using a checklist developed from the World Health Organization’s minimum injury surveillance core data set. Results The hospital data source was incomplete in areas of road user type, time of injury, mechanism of injury and place of injury. Thirteen case matches were identified between the two databases. Using the capture-recapture methods, the estimated road trauma events in Ntcheu district for the year 2018 is 954 (95% CI: 457, 1451) and an estimated 173 deaths (95% CI: 89, 257) in the same year. These estimates indicate 11% and 14% ascertainment of fatalities for the police and hospital data sources respectively.Discussion and conclusion There is significant underreporting for road deaths and injuries in Ntcheu district, which means there are critical data quality challenges in the respective data sources. It is therefore imperative for the road safety agencies and partners to resolve these data reporting and acquisition challenges.
Das «mittlere Einkommen» im Sinne von Art. 65 Abs. 1bis KVG
Authors: Petrik Andreas 
Published: July 2020

Besprechung des Urteils des Bundesgerichts 8C_228/2018 vom 22. Januar 2019 (zur Publikation vorgesehen)Das Bundesrecht sieht für untere und mittlere Einkommen eine prozentuale Verbilligung der Krankenkassenprämien von Kindern und jungen Erwachsenen in Ausbildung vor. Liegt die durch einen Kanton festgelegte Einkommensgrenze nur in einem geringen Ausmass über der Schwelle zwischen den unteren und mittleren Einkommen, ist dies mit den bundesrechtlichen Vorgaben nicht vereinbar.
Study on the organization of water-conservation activities and guiding strategies for urban communities based on public participation in China
Authors: Xue Binxia , Li Tongyu , Hou Xiaohe , Guo Jiayi 
Published: July 2020

Abstract
Water shortages and pollution in China are severe situations caused by rapid economic development and urbanization. The current water-conservation policies focus on implementing new technologies and management strategies at important spatial nodes while neglecting the significance of the response from the community and the public. In this paper, the elements that influence the public participation of water conservation within the community are analyzed and divided into three levels: the internal world elements of the residents, the different individual characters of the residents, and external world elements. Among these three levels, the internal world element level, including the desire to realize oneself, is crucial, as it will significantly strengthen an individual's participation activity once motivated. Based on Maslow's five-level theory of human needs, to establish a model of public participation in water-conservation communities, economic benefits, environmental improvement benefits, and the self-fulfilling satisfaction of participation will become the motivation for the model to function. In a new project, reclaimed water landscapes are established in the community, and eco-recreational activities with water-conservation themes are organized to guide and encourage public participation to foster water-conservation consciousness and to establish aging water-conservation guiding policies and implementation methods in the community.