bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oncologic results in rectal cancer patients with a subcentimeter distal margin after laparoscopic-assisted sphincter-preserving surgery: a propensity-score matched cohort analysis
Authors: Zhang Chenghai , Yang Hong , Cui Ming , Xing Jiadi , Yao Zhendan , Tan Fei , Zhang Nan , Chen Lei , Liu Maoxing , Xu Kai , Su Xiangqian 
Published: June 2020

Abstract
Background: Distal resection margin (DRM) is closely associated with sphincter-preserving surgery and oncologic safety for patients with mid-low rectal cancers. However, the optimal DRM has not been determined. The purpose of this study to assess the impact of a DRM of ≤ 1 cm on oncologic safetyMethods: Data of 378 rectal cancer patients who underwent laparoscopic-assisted sphincter-preserving surgery from 2009 to 2015 were retrospectively analyzed. Patients were divided into two groups based on DRM: ≤ 1 cm (n=74) and >1 cm (n=304). To minimize the differences between the two groups, propensity-score matching on baseline features was performed. Stratified analysis was performed according to neoadjuvant chemoradiotherapy.Results: Before propensity-score matching, no significant differences in 5-year disease-free survival (DFS) (92.8 vs. 81.3%; P=0.128) and 5-year overall survival (OS) (83.7 vs. 82.2%; p=0.892) were observed in patients with DRMs of ≤1(n=74) and >1cm (n=304), respectively. After propensity-score matching (1:1), there were also no significant differences in DFS (88.1 vs. 78.2%; P=0.162) and OS (84.5vs. 84.9%; P=0.420) between the DRM of ≤1 cm group (n=65) and >1 cm group (n=65), respectively. A total of 44 patients received preoperative chemoradiotherapy. In this cohort, the 5-year local recurrence (LR) rates ( p=0.118) and the 5-year DFS rates ( p=0.298) were not significantly different between two groups. A total of 334 patients received surgery without neoadjuvant chemoradiotherapy. There were also no significant differences in the 5-year LR rates ( p=0.150) and 5-year DFS rates ( p=0.172) between two groups.Conclusions: No matter whether patients with rectal cancers receiving neoadjuvant therapy or not, sphincter-preserving surgery with a DRM of ≤1 cm may be acceptable in mid-low rectal cancer without jeopardizing oncologic safety.
Genetic Engineering of Pseudomonas Chlororaphis Lzh-T5 to Enhance Production of Trans-2,3-Dihydro-3-Hydroxyanthranilic Acid
Authors: Li Ling , Li Zhenghua , Zhang Xuehong , Wang Wei , Huang Yujie , Wang Ruiming , Wang Tengfei , Liu Kaiquan 
Published: June 2020

Abstract
The authors have withdrawn this preprint due to erroneous posting.
MicroRNA Expression Signature and Target Prediction in Familial and Sporadic ACTH-Independent Macronodular Adrenal Hyperplasia
Authors: Xiaogang Tan , Zhu Jie , Liang Cui 
Published: June 2020

Abstract
Background: ACTH independent macronodular adrenal hyperplasia (AIMAH) is a rare disorder characterized by bilateral macronodular hyperplasia of the adrenal glands and increased cortisol production with subclinical or overt Cushing's syndrome. To screen and analyze the microRNA profile of AIMAH, so as to elucidate the possible pathogenesis of the disease. Methods: MiRNA microarray was used to test the tissue samples from familial AIMAH patients, sporadic AIMAH patients and normal controls of other non-tumor adrenocortical tissues, to identify characteristic microRNAs expression signatures. Some miRNAs were validated by RT-PCR. Furthermore, the key signal pathways and miRNAs involved in AIMAH pathogenesis were analyzed by gene ontology and pathway analysis.Results: Characteristic microRNA expression signatures were identified for familial AIMAH patients including 16 differentially expressed microRNAs and sporadic AIMAH patients including 8 differentially expressed microRNAs respectively. RT-PCR assay confirmed the chosed miRNAs expression, suggesting the high reliability of miRNA array. Pathway analysis showed that the most enriched pathway was renal cell carcinoma pathway. Overexpression of miR-17，miR-20a and miR-130b can inhibit glucocorticoid-induced apoptosis in the AIMAH pathogenesis.Conclusion: We have identified the miRNA signature in in Familial and Sporadic AIMAH patients, The differentially expressed miRNAs may be involved in the mechanisms of AIMAH pathogenesis. Specific miRNAs, such as miR-17，miR-20a and miR-130b may be new potential targets for further functional studies of AIMAH.
Cytological Effects of Herbicidal and Fungicidal Pesticides on Root Apex Meristem Cells of Triticum Aestivum
Authors: Menzyanova Natalia , Shishatskaya Ekaterina , Pyatina Svetlana , Volova Tatiana 
Published: June 2020

Abstract
Using the example of 2-day-old Triticum aestivum seedlings, grown in hydroponic culture, the cytological effects of pesticides with various mechanisms of the damaging were studied: the herbicides metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl and the fungicides tebuconazole, epoxiconazole, and azoxystrobin. All pesticides showed a dose-dependent inhibitory effect on the development of the roots of Triticum aestivum, but did not affect the mitotic activity and did not reduce the mitotic index of root apex meristem cells. Herbicides and fungicides affected the duration of metaphase, anophase, prophase, and cytokinesis activity in the root apex meristem of the wheat, and these changes were specific for the six studied preparations. Under the influence of all pesticides, the number of abnormal cells increased significantly, to the greatest extent (10–12 times compared with control - distilled water) under the influence of herbicides tribenuron-methyl and phenoxaprop-ethyl and fungicides azoxystrobin and epoxiconazole; to a lesser extent - under the influence of metribuzin and tebuconazole (6–7 times). For all studied pesticides the manifestation of cyto- and genotoxicity, including both clastogenic and aneugenic effects, was revealed in relation to the non-target plant Triticum aestivum. Under the influence of pesticides, structural anomalies, caused by chromosome breakdown (bridges, fragments, micronuclei) and spindle damage (lagging chromosomes and their fragments, uneven chromosome separation) were detected in the cells of the Triticum aestivum root apex meristem.
Intravenous Thrombolysis after Reversal of Dabigatran with Idarucizumab in Acute Ischemic Stroke: A Case Report
Authors: XIE DAN , Wang Xuefan , Li Yao , Chen Ruiling , Zhao Yingying , Xu Chunling , Zhang Qian , Zhang Yongbo 
Published: June 2020

Abstract
Background: As there is a growing concern about the cerebral embolism events secondary to non-valvular atrial fibrillation, direct oral anticoagulant (DOAC) has been more and more widely used as an anticoagulation treatment for prevention of stroke. However, in the face of life-threatening bleeding or emergency surgery/treatment, doac-related antagonists such as idarucizumab need to be urgently used to reverse the DOAC. Using rt-PA intravenous thrombolysis for acute ischemic stroke requires a time window of 4.5 hours. This case reports rt-PA intravenous thrombolysis after reversal of dabigatran anticoagulation with idarucizumab in patients with acute ischemic stroke. Case presentation: We report the case of 62-year-old Chinese female with nonvalvular atrial fibrillation treated with dabigatran 110mg twice daily, and missed a dose on the eve of the stroke. The patient presented with acute ischemic stroke causing the angle of mouth deviated to right side and left limb weakness in the early morning of the next day. Due to the last dosing time of dabigatran was between 24-48 hours, the patients were given rt-PA intravenous thrombolysis after reversal of dabigatran anticoagulation with idarucizumab, while all contraindications had been excluded by laboratory test and CT scan. NIHSS score was reduced from 4 to 1, and the patient was discharged after 2 weeks. Conclusion: Our case report adds to the evidence that idarucizumab administration is safe and effective in the setting of patients with atrial fibrillation treated with dabigatran who develop acute ischemic stroke requiring rt-PA intravenous thrombolysis.
Between Tales, Myths And Mysteries: Beliefs and Superstitions of A Quilombola Community From The Countryside of Northeastern Brazil
Authors: MACÊDO ERMÍNIA MEDEIROS , Barros Roseli Farias de Melo , Figueiredo Luciano Silva , Batista Márcio Luciano Pereira 
Published: June 2020

Abstract
Background: Tales, myths, beliefs and superstitions are part of the daily life of traditional communities, which strongly believe in the interference of both natural forces and activities, as well as supernatural ones in their social manifestations. Whether in the imaginary facts or real situations experienced by residents, this set of relationships and symbolisms underlie the cosmology of that social group, through which it is possible to identify traces of the cultural and identity constitution of a people. In quilombola communities, the recurrence of these cultural elements is a common practice. In this perspective, the objective was to recognize the tales, myths, beliefs and superstitions of the Quilombola Custaneira/Tronco community in the state of Piauí, as instruments for reaffirmation and strengthening of traditional knowledge and local culture. Method: The methodological path was based on the ethnographic approach and the life history through semi-structured interviews with 23 residents, being older or living longer in the locality. Results: By entering the cosmological universe of the Custaneira/Tronco community and accessing the accounts involving their tales, myths, beliefs and superstitions, we can identify, as in other quilombola communities, the presence of three worlds: the dead, the living and the enchanted.Conclusions: The conclusive aspects point out that the tales, myths, beliefs and superstitions, like the “Father of the Forest”, the visions and the “Mother of Water”, greatly influence the social and cultural practices of the community, especially in hunting rituals, in the relationship with animals and with local natural resources. The cosmological arrangement performs functions that organize its social structure and maintain the connection with its ancestors and its traditionality.
Land Cover Dynamics (from 1986 to 2015) and its Relation with Area Closure and Slope in Hita-Borkena Watershed, Northeastern Ethiopia
Authors: Ayele Alemayehu Assefa , Yimer Assefa Abegaz , Mini Simphiwe E. 
Published: June 2020

Abstract
Background: Land cover dynamics take place as a result of the integration of both physical and man-made factors. This situation is common in Ethiopia and in the study watershed as well. The study was intended to assess the land cover dynamics through 1986 – 2015 and to identify its relation with slope and landscape restoration that incorporates area closure. The analysis was made based on the interpretation of satellite images. Methods: Both ERDAS IMAGINE 9.1 and ARC GIS 10.3 software were employed in order to generate land cover maps of the study area from satellite images. Also, slope map of the study area was processed from DEM of Ethiopia. Results: The study found out that forestlands and shrublands shrunk through 1986 – 2015, grasslands expanded mainly due to the implementation of landscape restoration including area closure since 2001. Cropland expanded remarkably. Similarly, the coverage of bareland and settlement also increased. Conclusions: Based on the results of the study, it is concluded that similar restoration activities shall be implemented in similar environments in Ethiopia to keep the natural resources, and the vegetation cover of Hita-Borkena watershed shall be improved.
The hidden link between plasma mitochondrial DNA level and cardiac dysfunction after cardiopulmonary bypass
Authors: Fan Jing Xiu , Zeng Ling , Chen Lu , Li Ye , Pu Huqiong , Shen Jiayu , Kang Yan 
Published: June 2020

Abstract
Background: To investigate the hidden link between mitochondrial DNA (mtDNA) and cardiac function after cardiopulmonary bypass (CPB), we compared the relationship of plasma mtDNA level and the prevalence of cardiac dysfunction in patients receiving cardiac surgery with CPB.Methods: From September 1st, 2015 to December 1st, 2016, 962 patients who received cardiac surgery with CPB were prospectively comprised in this study. Routine blood test and examinations for blood biochemistry, NT-proBNP and mtDNA were arranged in half an hour when patients were transferred into intensive care unit (ICU). The clinical outcomes related to the cardiac function were determined by at least two senior doctors responsible for ICU. The data were inputted with double check and the inner logic of these data was set to establish the database. SAS 9.4, R 3.2.4 and SPSS 21.0 were used in data analysis.Results: Of the 962 patients (mean [SD] age, 51.6 [12.5] years; 459 [47.7%] male) comprised in this study, 692 patients received cardiac valve surgery, 83 patients received coronary artery bypass grafting (CABG), 58 patients received CABG and cardiac valve surgery at the same time, 70 patients received surgery on ascending aorta, 35 patients received surgery for simple congenital heart diseases and 15 patients received other types of surgery. The prevalence of cardiac dysfunction in this study is 9.25%. The level of mtRNA among patients with cardiac dysfunction and patients without cardiac dysfunction has no statistical significance (t=-1.42, P=0.1565). There was an obvious positive liner correlation between the level of plasma mtDNA and NT-proBNP in patients receiving CPB (r=0.1291, P=0.0386). There was an obvious positive liner correlation between the level of plasma mtDNA and APACHE II score in patients receiving CPB (r=0.2133, P<0.0001).Conclusions: The level of plasma mtDNA was closely related with the cardiac function and illness severity in patients receiving cardiac surgery with CPB.
Proteomic Profiling of Du145 Cell Line Exosomes Identifies Icam1 as Aputative molecular Mechanism by Which Relb Promotes The proliferation, Migration, and Invasion of Prostate cancer Cells
Authors: Li Wenjing , Xu Jingjing , Cheng Li , Zhang Lianjun , Shao Qiang , Guo Feng 
Published: June 2020

Abstract
Background: Prostate cancer (PC) is a serious health issue in men. Exosome plays essential roles in modulating the oncogenesis and progression of PC. RelB is highly expressed in PC and plays oncogenic parts in DU145 cells. We aim to uncover the protein panel of exosomes derived from RelB-knockdown DU145 cells (siRelB) as compared to control cells (sictrl) and explore a potential mechanism that RelB conferring the more aggressive phenotype to DU145 cells.Methods: Exosomes derived from sictrl and siRelB were subjected to Liquid Chromatography-Mass Spectrometry for proteomics profiling. Label-free quantification strategy iBAQ (intensity-based absolute quantification) was used to quantify and Fold Change (FC) was calculated to identify the differentially expressed proteins (DEPs). The characterization of proteins was conducted by bioinformatics analysis. ICAM1 over-expressing DU145 cells (hICAM1) and control cells (hctrl) were established by transfection using Lipofectamine 2000. The cell growth, migration, and invasion capabilities were measured by the xCelligence real-time monitoring system. Annexin V/PI-staining was adopted to assess apoptosis. CCK-8 assay was applied for proliferation evaluation. Integrin β-1, MMP9, and uPA were detected by Western blot.Results: 1259 exosomal proteins were identified, with 160 and 105 proteins unique to the siRelB and sictrl, respectively, while 994 proteins were present in both. We identified 137 upregulated and 55 downregulated proteins in siRelB. Gene Ontology (GO) analysis revealed that some DEPs had the cell adhesion molecular activity and participated in the cell adhesion process. Kyoto Encyclopedia of Genes and Genomes (KEGG) enriched that intercellular adhesion molecule-1 (ICAM1) was downregulated targeted by the NF-κB signaling. The FC of exosomal ICAM1 was 2.136. The expression of ICAM1 was positively related to RelB in PC by UALCAN. ICAM1 was shown to be co-expressed with RelB by GeneMANIA. The protein abundance of exosomal ICAM1 was lower in siRelB. hICAM1 had enhanced abilities of proliferation, migration, and invasion, with higher expression of Integrin β-1 when compared to hctrl.Conclusions: Our study identified 192 exosomal DEPs downstream of RelB in the DU145 cells. Exosomal ICAM1, conferring a more aggressive phenotype to DU145 cells, is a potential molecular mechanism modulating the tumorigenesis and progression of PC cells.
Characterization of the Fecal Microbiota in Gastrointestinal Cancer Patients and Healthy Controls
Authors: Li Ningning , Bai Chunmei , Guan Yuanzhi , Zhao Lin , Ge Yuping , Li Xiaoyuan 
Published: June 2020

Abstract
Background The incidence and mortality of gastrointestinal tumors are high in China. Some studies suggested that the gut microbiota is also related to the occurrence and development of tumors. At present, there are no prospective studies based on the correlation between gastrointestinal tumors and gut microbiota in the Chinese population. The objective of this report is to characterize the fecal microbiota in patients with esophageal cancer, gastric cancer, and colorectal cancer and healthy controls. Methods Patients with locally advanced or metastatic esophageal, gastric, and colorectal cancer were enrolled, and healthy people were included as controls. 16S rRNA sequencing was used to analyze the characteristics of fecal microbiota. PICRUSt software was used for functional prediction.Results Significant differences in the composition and abundance of fecal microbiota were identified between gastrointestinal cancer patients and healthy controls. The abundance of F. prausnitzii, C. clostridioforme, and B. adolescents in three different tumor groups was all significantly lower than in the control group (P<0.05). The abundance of R. gnavus, B. obeum, and R. faecis in the gastric and colorectal cancer groups was significantly lower than in the control group (P<0.05). The abundance of Dorea in the gastric cancer group was significantly lower than in the control group (P<0.05). B. fragilis, P. corpi, and E. coli were overabundant in the different tumor groups compared with the control (P<0.05). There were significant differences in functional metabolism and cell biological function between the tumor and control groups (P<0.05). The feces of cancer patients with more advanced staging had higher abundance of Prevotella and fewer Clostridium. Conclusions Patients with esophageal or gastric cancer had similar features of fecal bacteria as those with colorectal cancer. The metabolic function of fecal bacteria in the gastrointestinal cancer patients and the healthy controls were different.
Association of Serum Adipokines Levels with Glycemic Control and Metabolic Dyslipidemia in Sudanese Patients with Type 2 Diabetes Mellitus
Authors: Eltahir Halima Babiikir , Ali Elmahdi Mohamed , Mohamed Abdelrahim Osman 
Published: June 2020

Abstract
Background: The pathogenesis of type 2 diabetes mellitus is due to two major abnormalities including insulin resistance and dysfunction, which lead to the inability to regulate blood glucose level. Adiponectin is a hormone secreted by the adipose tissue and it takes part in glucose metabolism with insulin-sensitising properties. Low levels of adiponectin leads to reduction of fatty acid oxidation decreased glucose uptake in skeletal muscle cells and increased level of free fatty acids leading to insulin resistance. Leptin is another adipokine produced by adipose tissue involved in the control of food intake via its action on the hypothalamus, suppressing appetite and stimulating energy expenditure. Leptin plays a critical role in pathophysiology of type 2 diabetes mellitus.The aim of the study was to investigate the association of serum adipokines levels with glycemic control and metabolic dyslipidemia in Sudanese patients with type 2 diabetes mellitus.Methods: This was a case control study. 202 patients with type 2 diabetes and 102 non-diabetic controls participated after signing written consent. Weight (kg) and height (m) were measured then the body mass index (kg/m2) was determined. Blood samples were collected after an overnight fasting. FBG, HbA1c and lipid profiles were measured using enzymatic methods. Adiponectin and leptin were measured using sandwich ELISA.Results: Adiponectin concentrations was significantly lower in patients with type 2 diabetes compared with the controls (p<0.001) and it was inversely correlated with HbA1c (Pearson Correlation -.160, P value = 0.005), total cholesterol and LDL levels (P = 0.05) and direct correlated HDL levels (P = 0.05). Leptin concentrations was significantly higher in patients with type 2 diabetes compared with the controls (p<0.002) and it was positively correlated with HbA1c (Pearson Correlation .155, P value = 0.02), total cholesterol and LDL levels (P = 0.05), there were no correlation with HDL and TG levels. Patients had significantly higher fasting blood glucose, HbA1c levels, total cholesterol and LDL levels compared with the controls.Conclusion: Patients with type 2 diabetes mellitus had decreased levels of serum adiponectin, high levels of serum leptin. There were significant correlations found between adiponectin and leptin levels with glycemic control and metabolic dyslipidemia .
Prognostic Significance of SQSTM1 in Breast Cancer: A Comprehensive Analysis
Authors: Liu Yang , Du Qian , Sun Dan , Han Ruiying , Teng Mengmeng , Zhang Ying , Dong Yuzhu , Chen Siying , You Haisheng , Dong Yalin 
Published: June 2020

Abstract
Background: SQSTM1 (Sequestosome 1, p62) is degraded by activated autophagy and involved in the progression of in various types of cancers. However, the prognostic role and underlying regulation mechanism of SQSTM1 in the progression and development of breast cancer remain unclear.Methods: In this study, 1336 samples with available mRNA data from Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) database and 27 formalin fixation and paraffin embedding (FFPE) tissue samples from the First Affiliated Hospital of Xi’an Jiaotong University were collected to evaluate SQSTM1 expression in mRNA and protein levels. Kaplan–Meier and Cox regression were used for revealing prognostic value in three independent breast cancer independent datasets. Tumor Immune Estimation Resource (TIMER) database and Gene Set Variation Analysis (GSVA) was used to explore the relationship of SQSTM1 mRNA expression and immune infiltration level in breast cancer. Dysregulation mechanisms of SQSTM1 were also explored including copy number variation (CNV), somatic mutation, epigenetic alterations and other transcription and post-transcription level using multiple datasets. Finally, Gene Set Enrichment Analysis (GSEA) was constructed to elucidate functional regulating performance of SQSTM1 in breast cancer.Results: The results showed that mRNA and protein level of SQSTM1 were significantly elevated in breast cancer and receiver operating characteristic (ROC) curve showed that p62 may act as diagnostic biomarker. Lower expression of SQSTM1 predicted better outcome through multiple datasets. It was also found that SQSTM1 correlated with immune infiltrates in breast cancer. Moreover, CNV and methylation of SQSTM1 DNA was correlated with SQSTM1 dysregulation and act as prognostic factors for breast cancer patients. Yet, somatic mutation status of SQSTM1 didn’t show any prognostic relevance. We also identified diverse transcription factors that directly bound to SQSTM1 DNA and the miRNAs which may regulate SQSTM1 mRNA. Finally, functional enrichment analysis revealed that SQSTM1 is related to cell signal transduction, oxidative stress and autophagy in breast cancer.Conclusion: Our findings revealed that overexpression of SQSTM1 significantly to poor survival and immune infiltrations in breast cancer. In addition, SQSTM1 plays a key role in the progression of breast cancer and might be a promising biomarker for the diagnosis and personalized treatment of breast cancer patients.