bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Benefits of Dark Tourism Experience among Visitors in Malaysia
Authors: Jamin Aziean , Mohd Zain Zainab , Rosnita Sakarji Siti , Ahmad Nurbarirah , Mayang Delima Mohd Beta Raja 
Published: March 2020

Tourists are continuously searching for new trends, exciting ways of traveling and a variety of adrenaline rush experiences. Dark tourism is one of many examples of tourism products that able to fulfill these objectives. Although Malaysia has many dark tourism sites, they are not globally recognized yet and able to attract international tourists. Many focused on the negative impacts of the sites rather than the potential benefits of the industry. Thus, this study is prepared to investigate the benefits of dark tourism among visitors. The study adopted a model from Kang, Scott, Lee, and Ballantyne (2012). Three factors including social benefits, learning benefits, and psychological benefits were discussed. The findings have shown that dark tourism contributes sufficient benefits which may aid the rapid development of this tourism sector in the future.
Keywords: Dark tourism, Paranormal, Tourism trend, Malaysia
Bankruptcy Prediction Analysis of PT Garuda Indonesia Compared to Four Airlines Companies in Asia
Authors: Fitri Elda , Darmansyah Asep , Maya Damayanti Sylviana 
Published: March 2020

Airline industry is one of the promising industry for investors, it considered a big deal as it predicted to grow rapidly during for the next 20 years. Asia Pacific forecasted as the main player for airline in 2034, which will surpass Europe and North America. SKYTRAX Global Airlines Awards 2018 are announced Garuda Indonesia as one of the top ten best airlines in the world and the Best Cabin Crew in the world. The awards are followed by the other airlines in Asia. Nevertheless, airline industry also known for its volatility business, due to the big amount of operating cost, such as unstable fuel price and the high cost of labor. Those issues affect Indonesia’s best airlines, Garuda Indonesia, experienced loss for the past 5 consecutive years. The purpose of this research is to predict the bankruptcy possibilities of PT. Garuda Indonesia compared to five listed airlines company in Asia, which included as the best airlines in the world. This research will also analyze the relationship of Altman Z”-Score between PT. Garuda Indonesia to other companies by using Coefficient Correlation method. This research will be conducted by using Revised Altman Z” Score for non-manufacturers company which will predict the bankruptcy event for the next two or three years. The sample of this research are Garuda Indonesia (GIAA), Singapore Airlines (SING), THAI Airways (THAI), All Nippon Airways (ANA), and EVA Air (EVA) during the period of 2014-2018. Altman Z”-Score indicates that GIAA, THAI, and EVA predicted to bankrupt, while SING and ANA are predicted to be in the grey zone for the next 2 – 3 years.
Keywords: Airline Industry, Bankruptcy, Asia, Garuda Indonesia, Altman Z-Score
An Analysis of Economic Performance on Community Welfare
Authors: Yuyun Ka’arieni , . Meilida , Hukom Alexandra 
Published: March 2020

One of the ways in which the government in Central Kalimantan Province moves the regional economy is to develop an expansionary region which is expected to be able to further improve the economy of the community with the potential to support the pace of economic growth in the region for the welfare of its people. The aim of this study was to analyze The Economic Performance of Community Welfare in the Development District of Central Kalimantan in 2010-2017. This study uses a quantitative method and panel data. Proxy Economic Performance is a variable of Economic Growth, Unemployment Rate and Poverty Level as an independent variable, Community Welfare is proxied to be the Human Development Index as the dependent variable. The results of the study show that partially Economic Growth and Unemployment Rate do not significantly influence Community Welfare while the Poverty Level affects the Welfare of the Community. Simultaneously Economic Growth, Unemployment Rate,and Poverty Level have a significant effect on communityWelfare.

Keywords: Economic Performance; Economic Growth; Unemployment Rate; Poverty Level; Community Welfare
Radiation study techniques in diagnosing the causes of opisthorchiasis-induced obstructive jaundice
Authors: Klimova N. V. , Ilkanich A. Ya. , Darvin V. V. , Koctrubin A. L. , Vardanyan T. S. , Aliev F. S. 
Published: March 2020

Objective:to analyze the diagnostic value of radiation techniques in patients with opisthorchiasis-induced obstructive jaundice and to determine the types of bile duct (BD) changes characteristic of this disease.Subjects and methods.The investigation enrolled 103 patients with chronic opisthorchiasis complicated by obstructive jaundice. For BD visualization, the investigators used radiation diagnostic methods, such as ultrasonography (USG), magnetic resonance cholangiopancreatography (MRCP), and endoscopic retrograde cholangiopancreatography (ERCP); their efficiency was evaluated.Results.ERCP and MRCP could identify 5 types of BD architectonics in opisthorchiasis-induced obstructive jaundice. The sensitivity, specificity, and overall accuracy of MRCP in diagnosing opisthorchiasis-induced sclerotic changes were 98.1, 87.5, and 96.8%, respectively.Conclusion.Among instrumental methods for diagnosing sclerotic BD changes in prolonged opisthorchiasis invasion, it is preferable to use MRCP, which is determined by its high informative value. Five types of cholangioarchitectonics are detectable in chronic opisthorchiasis complicated by obstructive jaundice.
Difficulties of being young, reflections on anti‑aging
Authors: Mojs Ewa 
Published: March 2020

Youth is undeniably a positive value, something that we consider to be good and which we would like to possess forever. However, time prevents us from it. We are getting older. Ageing is a process affecting every living organism, without exception, and death is a natural part of life. The aim of this work is to present cultural and psychological conditions for the development of aesthetic medicine. Progress in this area contributes to achieving the objectives of maintaining a young appearance. However, the technology development may result in some health issues. The use of aesthetic medicine treatments is influenced by motivational mechanisms, which are responsible for activating, directing, maintaining and ending the behaviour.Sometimes, however, these mechanisms are disrupted and there may be an increase in forced activities and addiction. Advances in medicine therefore require the development of gold standards of practice. They should be aimed at improving people's health, but also at stopping the aging process, prolonging life, adding healthy years to life. Therefore, we should look for methods of preserving youth, physical, mental, emotional, naturalness, activity and self‑awareness. And these should not be separable objectives.
Technology Focus: History Matching and Forecasting (April 2020)
Authors: Emerick Alexandre 
Published: March 2020

Technology Focus
In project management, there is a model known as the quality triangle, which states that a project is constrained by three aspects: quality, time, and cost. Usually, one of these constraints is fixed. For example, if the cost is fixed, the quality of the project will depend heavily on the time available for the conclusion. Of course, this is an idealized and incomplete model of reality, but it has its merits.
In the course of my career, I have had the opportunity to work on sever-al history-matching cases. Every time I think about these cases, the quality triangle comes to mind. Most history-matching studies have fixed resources—that is, the team of engineers and geoscientists is predetermined. (So, the cost vertex of the triangle is settled.) Moreover, the deadlines are always very strict. This constrained scenario often leads to an unfortunate result: The quality of the study suffers. In some cases, this quality reduction comes in the form of a reduction in scope. For example, instead of matching data from all wells of the reservoir, we limit the analysis to the wells near the area of interest of the project. However, we must remember that history matching is never the end of the history. Creeping the quality in a reservoir study can result in very serious consequences for a project by harming the decision-making, which surely can cost a lot of money.
So, what is the way around this problem? The answer is twofold: people and technology. Training is important, and integration is paramount. A talented and engaged team is the main component for success. Technology is the other component. Recent advances in seismic inversion, geostatistics, reservoir simulation, assisted history matching, and high-performance computing (just to mention a few) should always be on the table. In good hands, they can make a difference.
As usual, we had a nice collection of papers on history matching and forecasting published this past year. This feature summarizes some good examples, but you can find many other interesting papers on the OnePetro.org online library. I hope you enjoy the reading.
Recommended additional reading at OnePetro: www.onepetro.org.
IPTC 19128 Benchmarking of State-of-the-Art Assisted History-Matching Methods Under Reservoir Uncertainty on a Complex Waterflooding Process by Marko Maucec, Saudi Aramco, et al.
SPE 195549 A Proper Data Comparison for Seismic History-Matching Processes by Alessandra Davolio, University of Campinas, et al.
SPE 195837 Methods for Probabilistic Uncertainty Quantification With Reliable Subsurface Assessment and Robust Decision-Making by Shusei Tanaka, Chevron, et al.
Genomic heterogeneity of Dichelobacter nodosus within and between UK sheep flocks and between age groups within a flock
Authors: DAVIES PEERS , Blanchard Adam , Staley Ceri , Bollard Nikki , Coffey Tracey , Tötemeyer Sabine 
Published: March 2020

Abstract
Background Footrot and interdigital dermatitis are endemic infectious diseases in all sheep farming regions, impairing welfare and production. The development of efficacious vaccines against the primary causative pathogen has been hampered by the extensive antigenic diversity of Dichelobacter nodosus . Understanding the heterogeneity of the pathogen within and between flocks is essential if the feasibility of bespoke vaccine production is to be assessed for use in the U.K. Results In this study 56 ewe and lamb isolates from 9 flocks were compared by D. nodosus serogroup and Multi Locus Sequence Type which provides significantly enhanced discriminatory power for molecular epidemiology. Serogroup heterogeneity between flocks ranged from two to five unique serogroups per flock. Three flocks contained isolates of two serogroups, two flocks contained isolates of three serogroups and one flock included isolates of five serogroups. Analysis of 25 isolates from one flock with high prevalence of lameness, identified that serogroup and sequence type was significantly correlated with age. Significantly higher proportion of lambs were infected with serogroup B (principally ST85) as opposed to serogroup H (principally ST86), which predominated amongst adult sheep. Conclusions Genomic heterogeneity of the pathogen was significantly lower within flock compared to heterogenicity observed between flocks. Furthermore, this study indicates that within a flock, the host-pathogen dynamics and susceptibility to particular D. nodosus strains may be age dependent. Keywords D. nodosus; Footrot; interdigital dermatitis; lameness; sheep; MLST; Serogroup; molecular epidemiology
Comparison of methods for the detection of in vitro synergy in multidrug-resistant Gram-negative bacteria
Authors: Gaudereto Juliana Januario , Neto Lauro Vieira Perdigão , Leite Gleice Cristina , Sanchez Evelyn , Martins Roberta Cristina Ruedas , Prado Gladys Villas Boas do , Rossi Flavia , Guimarães Thais , Levin Anna Sara , costa silvia figueiredo 
Published: March 2020

Abstract
Background
The use of combined antibiotic therapy has become an option for infections caused by multidrug-resistant (MDR) bacteria. The time-kill (TK) assay is considered the gold standard method for the evaluation of in vitro synergy, but it is a time-consuming and expensive method.
The purpose of this study was to evaluate two methods for testing in vitro antimicrobial combinations: the disk diffusion method through disk approximation (DA) and the agar gradient diffusion method via the MIC:MIC ratio. The TK assay was included as the gold standard. MDR Gram-negative clinical isolates (n=62; 28 Pseudomonas aeruginosa , 20 Acinetobacter baumannii , and 14 Serratia marcescens ) were submitted to TK, DA, and MIC:MIC ratio synergy methods.
Results
Overall, the agreement between the DA and TK assays ranged from 20 to 93%. The isolates of A. baumannii showed variable results of synergism according to TK, and the calculated agreement was statistically significant in this species against fosfomycin with meropenem including colistin-resistant isolates. The MIC:MIC ratiometric agreed from 35 to 71% with TK assays. The kappa test showed good agreement for the combination of colistin with amikacin (K = 0.58; P = 0.04) among the colistin-resistant A. baumannii isolates.
Conclusions
The DA and MIC:MIC ratiometric methods are easier to perform and might be a more viable tool for clinical microbiology laboratories.
Transcriptomic and proteomic analyses of ovarian follicles reveal the role of VLDLR in chicken follicle selection
Authors: Chen Qiuyue , Wang Yiya , Liu Zemin , Guo Xiaoli , Sun Yi , Kang Li , Jiang Yunliang 
Published: March 2020

Abstract
Background: Follicle selection in chickens refers to the process of selecting one follicle from a group of small yellow follicles (SY, 6-8 mm in diameter) for development into 12-15 mm hierarchical follicles (usually F6 follicles), which is an important process affecting laying performance in the poultry industry. Although transcriptomic analysis of chicken ovarian follicles has been reported, integrated analysis of chicken follicles for selection by using both transcriptomic and proteomic approaches is still rarely performed. In this study, we compared the proteomes and transcriptomes of SY and F6 follicles in laying hens and identified several genes involved in chicken follicle selection.
Results: Transcriptomic analysis revealed 855 differentially expressed genes (DEGs) between SY follicles and F6 follicles in laying hens, among which 202 were upregulated and 653 were downregulated. Proteomic analysis revealed 259 differentially expressed proteins (DEPs), including 175 upregulated and 84 downregulated proteins. Among the identified DEGs and DEPs, changes in the expression of seven genes, including VLDLR1, WIF1, NGFR, AMH, BMP15, GDF6 and MMP13 , and nine proteins, including VLDLR, VTG1, VTG3, PSCA, APOB, APOV1, F10, ZP2 and ZP3L2, were validated. Further analysis indicated that the mRNA level of chicken VLDLR was higher in F6 follicles than in SY follicles and was also higher in granulosa cells (GCs) than in thecal cells (TCs), and it was stimulated by FSH in GCs of prehierarchical follicles.
Conclusions: By comparing the proteomes and transcriptomes of SY and F6 follicles in laying hens, we identified several differentially expressed proteins/genes that might play certain roles in chicken follicle selection. These data may contribute to the identification of functional genes and proteins involved in chicken follicle selection.
High-throughput Sequencing Study of the Effect of Transabdominal Hysterectomy on Intestinal Flora in Patients with Uterine Fibroids
Authors: Wang Wantong , Li Yibing , Wu Qijun , Pan Xin , He Xinhui , Ma Xiaoxin 
Published: March 2020

Abstract
Background: To investigate the effect of transabdominal hysterectomy on the diversity of the intestinal flora in patients with uterine fibroids. Patients with uterine fibroids were selected from September 2018 to December 2018, in the Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, and stool specimens were collected from patients before and after surgery.

Results :High-throughput sequencing of the 16S rRNA gene was used to detect the changes in microbial community structure and diversity, and the effects of total hysterectomy on the intestinal flora were further analyzed. Estrogen levels decreased after trans-abdominal hysterectomy. High-throughput sequencing showed that after abdominal hysterectomy, the abundance and diversity of the intestinal flora decreased. The abundance changes were mainly due to Proteobacteria, where their abundance increased.

Conclusions :Trans-abdominal hysterectomy changes the intestinal flora of the body by lowering the level of estrogen in the body, which reduces the diversity and abundance of the intestinal flora .

Key words: intestinal flora, uterine fibroids, hysterectomy, estrogen,high-throughput sequencing,16sRNA
Australian Aedes aegypti mosquitoes are susceptible to infection with a highly divergent and sylvatic strain of dengue virus type 2 but are unlikely to transmit it
Authors: Pickering Paul , Hugo Leon , Devine Gregor J , Aaskov John G , Liu wenjun 
Published: March 2020

Abstract
Background: Humans are the primary hosts of dengue viruses (DENV). However, sylvatic cycles of transmission can occur among non-human primates and human encroachment into forested regions can be a source of emergence of new strains such as the highly divergent and sylvatic strain of DENV2, QML22, recovered from a dengue fever patient returning to Australia from Borneo. The objective of the present study was to evaluate the vector competence of Australian Aedes aegypti (Ae. aegypti) mosquitoes for this virus.

Methods: Four to five day old mosquitoes from two strains of Ae. aegypti from Queensland, Australia, were feed a meal of sheep blood containing 108 50% cell culture infectious dose per ml (CCID50/ml) of either QML22 or an epidemic strain of DENV serotype 2 (QML16) isolated from a dengue fever patient in Australia in 2015. Mosquitoes were maintained at 28°C, 75% relative humidity and sampled 7, 10 and 14 days post-infection (DPI). Live virions in mosquito bodies (abdomen/thorax), legs and wings and saliva expectorates from individual mosquitoes were quantified using a Cell Culture Enzyme-linked Immunosorbant Assay (CCELISA) to determine infection, dissemination and transmission rates.

Findings: The infection and dissemination rates of the sylvatic DENV2 strain, QML22, were significantly lower than that for QML16. While the titres of virus in the bodies of mosquitoes infected with either of these viruses were similar, titres in legs and wings were significantly lower in mosquitoes infected with QML22 at most time points although they reached similar levels by 14 DPI. QML16 was detected in 16% (n = 25) and 28% (n = 25) of saliva expectorates at 10 and 14 DPI, respectively. In contrast, no virus was detected in the saliva expectorates of QML22 infected mosquitoes.

Conclusions: Australia urban/peri-urban Ae. aegypti species are susceptible to infection by the sylvatic and highly divergent DENV 2 QML22 but replication of QML22 is attenuated relative to the contemporary strain, QML16. A salivary gland infection or escape barrier may be acting to prevent infection of saliva and would prevent onward transmission of this highly divergent virus in Australia.
Assessment of risk factors for early childhood caries at different ages in Shandong, China and reflections on oral health education: a cross-sectional study
Authors: Zhang Meng , Zhang Xinyue , Zhang Yuan , Li Yanan , Shao Chunchun , Xiong Shijiang , Lan Jing , Wang Zhifeng 
Published: March 2020

Abstract
Background: The high prevalence of early childhood caries (ECC) is widespread around the world, and oral health education (OHE) plays a vital role in preventing ECC. Numerous studies on ECC risk factor assessment have assisted us in enriching the content of OHE. The objective of this study was to further assess independent risk factors for ECC at different ages to provide evidence and insights for OHE.Methods: Children aged 3-5 years old (N=1301) in Shandong Province were enrolled in this cross-sectional study. Data about oral health status and caregivers’ oral health knowledge, attitude, and practice (KAP) were extracted from the 4th National Oral Health Survey of China. The associations between ECC prevalence and various KAP variables were tested with chi-square tests, bivariate analysis and multivariable logistic regression analyses.Results: The ECC prevalence in Shandong Province was 64.6%, and the dmft mean was 3.15. The independent variables with an increased risk for ECC were age, feeding method within 6 months of birth, bedtime sugar frequency, experience of toothache over the past year and dental visits (P<0.05, chi-square tests). Complete breastfeeding within 6 months of birth primarily contributed to the high ECC risk of the 3-year-old group (OR: 3.39, 95% CI: 1.41-8.17), while high frequency bedtime sweet consumption mainly contributed to that of the 5-year-old group (OR: 3.22, 95% CI: 1.03-10.06; logistic regression analysis). Tooth brushing was not associated with ECC in this study, and some positive knowledge and attitude variables were positively correlated with a high risk of ECC.Conclusion: These data provide evidence to suggest that the ECC-related risk factors at different ages are inconsistent, which provides some insights for OHE. We should highlight the effects of feeding methods in the early stages of deciduous dentition and sugar habits in the late stages of deciduous dentition on ECC, as well as encourage preventive dental visit and supplemental training for oral health practices.