bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response of soybean and corn to halauxifen-methyl
Authors: Quinn Jessica , Soltani Nader , Ashigh Jamshid , Hooker David C. , Robinson Darren E. , Sikkema Peter H. 
Published: February 2020

AbstractPreplant (PP) herbicide applications are an important tool within an integrated weed management system, specifically in no-till production. An understanding of crop tolerance regarding PP applications is important for effectively integrating a new herbicide into no-till cropping systems. Twelve field trials (six in corn and six in soybean) were conducted over a 2-yr period (2018 and 2019) near Exeter and Ridgetown, ON. The purpose of these studies was to evaluate the tolerance of soybean and corn to halauxifen-methyl applied PP, PRE, or POST at the registered rate (5 g a.i. ha−1) and twice the registered rate (10 g a.i. ha−1), hereafter referred to as the 1× and 2× rate, respectively. All trials were kept weed-free throughout the growing season to remove the confounding effect of weed interference. Halauxifen-methyl applied 14 d preplant (DPP), 7 DPP, 1 DPP, and 5 d after seeding (DAS) at the 1× and 2× rates caused ≤10% visible soybean injury. In contrast, halauxifen-methyl applied POST (cotyledon–unifoliate stage, VE-VC) caused 67% to 87% visible soybean injury, a 50% to 53% reduction in height, 65% to 81% decrease in population, 56% to 67% lower biomass, and 53% to 63% decline in yield. Halauxifen-methyl applied 10 DPP, 5 DPP, 1 DPP, 5 DAS, and POST (spike–one leaf stage, VE-V1) at the 1× and 2× rate caused ≤3% visible corn injury and caused no effect on corn height or biomass. Halauxifen-methyl applied at VE-V1 at the 2× rate reduced corn yield 10%. Based on these studies, the current application restriction of 7 DPP in soybean and 5 DPP in corn is conservative and could be expanded. Expanding the application window of halauxifen-methyl would increase the utility of this herbicide for producers.
Multiscale MHD‐Kinetic PIC Study of Energy Fluxes Caused by Reconnection
Authors: Lapenta Giovanni , El Alaoui Mostafa , Berchem Jean , Walker Raymond 
Published: February 2020

AbstractWe present an analysis of the energy partitioning in the magnetotail during a substorm at 03:58:00 UT on 7 February 2009. The analysis employs a multiscale approach where we use a state from a global magnetohydrodynamics (MHD) model to spawn a kinetic particle‐in‐cell (PIC) simulation of a large portion of the tail. We directly investigate the energy fluxes resulting from magnetic reconnection. The kinetic run provides information on the additional processes absent in the MHD description. The ion bulk energy and enthalpy fluxes carry the greatest energy, but the Poynting flux and electron enthalpy flux also carry a significant portion. The other fluxes (e.g., heat flux) are relatively small but are especially important because they allow us to identify the extra processes present only in the kinetic description. The energy fluxes present in the MHD approximation (Poynting flux, enthalpy flux, and bulk energy flux) are quantitatively accurate, and the kinetic correction does not greatly alter the MHD picture. However, there are two unique effects resulting from the kinetic physics. First, the formation of a rarefaction of the plasma flow into the reconnection site leads to a progressive decline in time of the particle energy fluxes with respect to the Poynting flux. Second, we observe that the instabilities developing in the kinetic reconnection outflows form structures absent from the MHD description. These structures reveal themselves as fluctuations within the energy fluxes. Especially notable are regions of inverted heat flux, where the heat flux is in the opposite direction to the total energy and mass flow.
Development, Productivity, and Seasonality of Living Planktonic Foraminiferal Faunas and Limacina helicina in an Area of Intense Methane Seepage in the Barents Sea
Authors: Ofstad Siri , Meilland Julie , Zamelczyk Katarzyna , Chierici Melissa , Fransson Agneta , Gründger Friederike , Rasmussen Tine L. 
Published: February 2020

AbstractAlthough the plankton communities in the Barents Sea have been intensely studied for decades, little is known about the living planktonic foraminiferal (LPF) and pteropod faunas, especially those found at methane seep sites. Along a repeated transect in the “crater area” (northern Barents Sea, 74.9°N, 27.7°E) in spring and summer 2016 the flux of LPF and of the pteropod species Limacina helicina showed a high degree of variability. The LPF had low concentration (0–6 individuals m−3) and small tests (x̄ = 103.3 μm) in spring and a 53‐fold increase (43–436 individuals m−3) and larger tests (x̄ = 188.6 μm) in summer. Similarly, the concentration of L. helicina showed a tenfold increase between spring and summer. The LPF species composition remained stable with the exception of the appearance of subtropical species in summer. No relationship was observed between the spatial distribution of LPF, L. helicina, and methane concentrations in the area. The methane plumes in April coincided with elevated dissolved inorganic carbon, low pH, and calcium carbonate saturation states, and the methane concentration seemed to be controlled by lateral advection. The δ13C and δ18O of Neogloboquadrina pachyderma and Turborotalita quinqueloba are comparable to previous observations in the Arctic and do not show any influence of methane in the isotopic signals of the shells. Although no evidence of direct impact of high methane concentrations on the LPF (size and concentration) were found, we speculate that methane could indirectly enhance primary productivity, and thus biomass, through several potential pathways.
On-chip parallel Fourier transform spectrometer for broadband selective infrared spectral sensing
Authors: Fathy Alaa , Sabry Yasser M. , Nazeer Sébastien , Bourouina Tarik , Khalil Diaa A. 
Published: February 2020

AbstractOptical spectrometers enable contactless chemical analysis. However, decreasing both their size and cost appears to be a prerequisite to their widespread deployment. Chip-scale implementation of optical spectrometers still requires tackling two main challenges. First, operation over a broad spectral range extending to the infrared is required to enable covering the molecular absorption spectrum of a broad variety of materials. This is addressed in our work with an Micro-Electro Mechanical Systems (MEMS)-based Fourier transform infrared spectrometer with an embedded movable micro-mirror on a silicon chip. Second, fine spectral resolution Δλ is also required to facilitate screening over several chemicals. A fundamental limit states that Δλ is inversely proportional to the mirror motion range, which cannot exceed the chip size. To boost the spectral resolution beyond this limit, we propose the concept of parallel (or multi-core) FTIR, where multiple interferometers provide complementary optical paths using the same actuator and within the same chip. The concept scalability is validated with 4 interferometers, leading to approximately 3 times better spectral resolution. After the atmospheric contents of a greenhouse gas are monitored, the methane absorption bands are successfully measured and discriminated using the presented device.
An aptamer-based shear horizontal surface acoustic wave biosensor with a CVD-grown single-layered graphene film for high-sensitivity detection of a label-free endotoxin
Authors: Ji Junwang , Pang Yiquan , Li Dongxiao , Huang Zheng , Zhang Zuwei , Xue Ning , Xu Yi , Mu Xiaojing 
Published: February 2020

AbstractThe thickness of the sensitive layer has an important influence on the sensitivity of a shear horizontal surface acoustic wave (SH-SAW) biosensor with a delay-line structure and lower layer numbers of graphene produce better sensitivity for biological detection. Therefore, a label-free and highly sensitive SH-SAW biosensor with chemical vapor deposition (CVD-)-grown single-layered graphene (SLG) for endotoxin detection was developed in this study. With this methodology, SH-SAW biosensors were fabricated on a 36° Y-90° X quartz substrate with a base frequency of 246.2 MHz, and an effective detection cell was fabricated using acrylic material. To increase the surface hydrophilicity, chitosan was applied to the surface of the SLG film. Additionally, the aptamer was immobilized on the surface of the SLG film by cross-linking with glutaraldehyde. Finally, the sensitivity was verified by endotoxin detection with a linear detection ranging from 0 to 100 ng/mL, and the detection limit (LOD) was as low as 3.53 ng/mL. In addition, the stability of this type of SH-SAW biosensor from the air phase to the liquid phase proved to be excellent and the specificity was tested and verified by detecting the endotoxin obtained from Escherichia coli (E. coli), the endotoxin obtained from Pseudomonas aeruginosa (P. aeruginosa), and aflatoxin. Therefore, this type of SH-SAW biosensor with a CVD-grown SLG film may offer a promising approach to endotoxin detection, and it may have great potential in clinical applications.
Crenigacestat, a selective NOTCH1 inhibitor, reduces intrahepatic cholangiocarcinoma progression by blocking VEGFA/DLL4/MMP13 axis
Authors: Mancarella Serena , Serino Grazia , Dituri Francesco , Cigliano Antonio , Ribback Silvia , Wang Jingxiao , Chen Xin , Calvisi Diego F. , Giannelli Gianluigi 
Published: February 2020

AbstractIntrahepatic cholangiocarcinoma (iCCA) is a deadly disease with rising incidence and few treatment options. An altered expression and/or activation of NOTCH1–3 receptors has been shown to play a role in iCCA development and progression. In this study, we established a new CCA patient-derived xenograft model, which was validated by immunohistochemistry and transcriptomic analysis. The effects of Notch pathway suppression by the Crenigacestat (LY3039478)-specific inhibitor were evaluated in human iCCA cell lines and the PDX model. In vitro, LY3039478 significantly reduced Notch pathway components, including NICD1 and HES1, but not the other Notch receptors, in a panel of five different iCCA cell lines. In the PDX model, LY3039478 significantly inhibited the Notch pathway and tumor growth to the same extent as gemcitabine. Furthermore, gene expression analysis of iCCA mouse tissues treated with LY3039478 revealed a downregulation of VEGFA, HES1, and MMP13 genes. In the same tissues, DLL4 and CD31 co-localized, and their expression was significantly inhibited in the treated mice, as it happened in the case of MMP13. In an in vitro angiogenesis model, LY3039478 inhibited vessel formation, which was restored by the addition of MMP13. Finally, RNA-sequencing expression data of iCCA patients and matched surrounding normal liver tissues downloaded from the GEO database demonstrated that NOTCH1, HES1, MMP13, DLL4, and VEGFA genes were significantly upregulated in tumors compared with adjacent nontumorous tissues. These data were confirmed by our group, using an independent cohort of iCCA specimens. Conclusion: We have developed and validated a new iCCA PDX model to test in vivo the activity of LY3039478, demonstrating its inhibitory role in Notch-dependent angiogenesis. Thus, the present data provide new knowledge on Notch signaling in iCCA, and support the inhibition of the Notch cascade as a promising strategy for the treatment of this disease.
A novel nonsense mutation of ERCC2 in a Vietnamese family with xeroderma pigmentosum syndrome group D
Authors: Bui Chi-Bao , Duong Thao Thi Phuong , Tran Vien The , Pham Thuy Thanh T. , Vu Tung , Chau Gia Cac , Vo Thanh-Niem Van , Nguyen Vinh , Trinh Dieu-Thuong Thi , Hoang Minh Van 
Published: February 2020

AbstractXeroderma pigmentosum (XP) group D, a severe disease often typified by extreme sun sensitivity, can be caused by ERCC2 mutations. ERCC2 encodes an adenosine triphosphate (ATP)-dependent DNA helicase, namely XP group D protein (XPD). The XPD, one of ten subunits of the transcription factor TFIIH, plays a critical role in the nucleotide-excision repair (NER) pathway. Mutations in XPD that affect the NER pathway can lead to neurological degeneration and skin cancer, which are the most common causes of death in XP patients. Here, we present detailed phenotypic information on a Vietnamese family in which four members were affected by XP with extreme sun sensitivity. Genomic analysis revealed a compound heterozygous mutation of ERCC2 that affected family members and single heterozygous mutations in unaffected family members. We identified a novel, nonsense mutation in one allele of ERCC2 (c.1354C > T, p.Q452X) and a known missense mutation in the other allele (c.2048G > A, p.R683Q). Fibroblasts isolated from the compound heterozygous subject also failed to recover from UV-driven DNA damage, thus recapitulating aspects of XP syndrome in vitro. We describe a novel ERCC2 variant that leads to the breakdown of the NER pathway across generations of a family presenting with severe XP.
The hornwort genome and early land plant evolution
Authors: Zhang Jian , Fu Xin-Xing , Li Rui-Qi , Zhao Xiang , Liu Yang , Li Ming-He , Zwaenepoel Arthur , Ma Hong , Goffinet Bernard , Guan Yan-Long , Xue Jia-Yu , Liao Yi-Ying , Wang Qing-Feng , Wang Qing-Hua , Wang Jie-Yu , Zhang Guo-Qiang , Wang Zhi-Wen , Jia Yu , Wang Mei-Zhi , Dong Shan-Shan , Yang Jian-Fen , Jiao Yuan-Nian , Guo Ya-Long , Kong Hong-Zhi , Lu An-Ming , Yang Huan-Ming , Zhang Shou-Zhou , Van de Peer Yves , Liu Zhong-Jian , Chen Zhi-Duan 
Published: February 2020

AbstractHornworts, liverworts and mosses are three early diverging clades of land plants, and together comprise the bryophytes. Here, we report the draft genome sequence of the hornwort Anthoceros angustus. Phylogenomic inferences confirm the monophyly of bryophytes, with hornworts sister to liverworts and mosses. The simple morphology of hornworts correlates with low genetic redundancy in plant body plan, while the basic transcriptional regulation toolkit for plant development has already been established in this early land plant lineage. Although the Anthoceros genome is small and characterized by minimal redundancy, expansions are observed in gene families related to RNA editing, UV protection and desiccation tolerance. The genome of A. angustus bears the signatures of horizontally transferred genes from bacteria and fungi, in particular of genes operating in stress-response and metabolic pathways. Our study provides insight into the unique features of hornworts and their molecular adaptations to live on land.
Survey data on factors that influence the adoption of soil carbon enhancing practices in Western Kenya
Authors: Kanyenji George Magambo , Ng’ang’a Stanley Karanja , Girvetz Evan Hartunian 
Published: February 2020

AbstractThe data described in this paper were collected in Western Kenya, specifically in Kakamega and Vihiga Counties. The data were collected from 334 households with the aim of assessing factors that facilitate or constrain the adoption of practices that enhance the sequestration of soil carbon. The data were collected through a structured questionnaire that was designed in SurveyCTO. The data were later downloaded from SurveyCTO servers and exported to STATA version 14 for cleaning and analysis. This data can be used by researchers to assess the probability and extent of adoption of specific soil carbon enhancing practices in the two counties of Western Kenya. Additionally, it can be utilized to access the impact of adopting soil carbon enhancing practices on maize and beans yield at both the plot and the farm level.
Gaze, visual, myoelectric, and inertial data of grasps for intelligent prosthetics
Authors: Cognolato Matteo , Gijsberts Arjan , Gregori Valentina , Saetta Gianluca , Giacomino Katia , Hager Anne-Gabrielle Mittaz , Gigli Andrea , Faccio Diego , Tiengo Cesare , Bassetto Franco , Caputo Barbara , Brugger Peter , Atzori Manfredo , Müller Henning 
Published: February 2020

AbstractA hand amputation is a highly disabling event, having severe physical and psychological repercussions on a person’s life. Despite extensive efforts devoted to restoring the missing functionality via dexterous myoelectric hand prostheses, natural and robust control usable in everyday life is still challenging. Novel techniques have been proposed to overcome the current limitations, among them the fusion of surface electromyography with other sources of contextual information. We present a dataset to investigate the inclusion of eye tracking and first person video to provide more stable intent recognition for prosthetic control. This multimodal dataset contains surface electromyography and accelerometry of the forearm, and gaze, first person video, and inertial measurements of the head recorded from 15 transradial amputees and 30 able-bodied subjects performing grasping tasks. Besides the intended application for upper-limb prosthetics, we also foresee uses for this dataset to study eye-hand coordination in the context of psychophysics, neuroscience, and assistive robotics.
Nuclear Translocation of Glutaminase GLS2 in Human Cancer Cells Associates with Proliferation Arrest and Differentiation
Authors: López de la Oliva Amada R. , Campos-Sandoval José A. , Gómez-García María C. , Cardona Carolina , Martín-Rufián Mercedes , Sialana Fernando J. , Castilla Laura , Bae Narkhyun , Lobo Carolina , Peñalver Ana , García-Frutos Marina , Carro David , Enrique Victoria , Paz José C. , Mirmira Raghavendra G. , Gutiérrez Antonia , Alonso Francisco J. , Segura Juan A. , Matés José M. , Lubec Gert , Márquez Javier 
Published: February 2020

AbstractGlutaminase (GA) catalyzes the first step in mitochondrial glutaminolysis playing a key role in cancer metabolic reprogramming. Humans express two types of GA isoforms: GLS and GLS2. GLS isozymes have been consistently related to cell proliferation, but the role of GLS2 in cancer remains poorly understood. GLS2 is repressed in many tumor cells and a better understanding of its function in tumorigenesis may further the development of new therapeutic approaches. We analyzed GLS2 expression in HCC, GBM and neuroblastoma cells, as well as in monkey COS-7 cells. We studied GLS2 expression after induction of differentiation with phorbol ester (PMA) and transduction with the full-length cDNA of GLS2. In parallel, we investigated cell cycle progression and levels of p53, p21 and c-Myc proteins. Using the baculovirus system, human GLS2 protein was overexpressed, purified and analyzed for posttranslational modifications employing a proteomics LC-MS/MS platform. We have demonstrated a dual targeting of GLS2 in human cancer cells. Immunocytochemistry and subcellular fractionation gave consistent results demonstrating nuclear and mitochondrial locations, with the latter being predominant. Nuclear targeting was confirmed in cancer cells overexpressing c-Myc- and GFP-tagged GLS2 proteins. We assessed the subnuclear location finding a widespread distribution of GLS2 in the nucleoplasm without clear overlapping with specific nuclear substructures. GLS2 expression and nuclear accrual notably increased by treatment of SH-SY5Y cells with PMA and it correlated with cell cycle arrest at G2/M, upregulation of tumor suppressor p53 and p21 protein. A similar response was obtained by overexpression of GLS2 in T98G glioma cells, including downregulation of oncogene c-Myc. Furthermore, human GLS2 was identified as being hypusinated by MS analysis, a posttranslational modification which may be relevant for its nuclear targeting and/or function. Our studies provide evidence for a tumor suppressor role of GLS2 in certain types of cancer. The data imply that GLS2 can be regarded as a highly mobile and multilocalizing protein translocated to both mitochondria and nuclei. Upregulation of GLS2 in cancer cells induced an antiproliferative response with cell cycle arrest at the G2/M phase.
Theoretical Model of Helium Bubble Growth and Density in Plasma-Facing Metals
Authors: Hammond Karl D. , Maroudas Dimitrios , Wirth Brian D. 
Published: February 2020

AbstractWe present a theoretically-motivated model of helium bubble density as a function of volume for high-pressure helium bubbles in plasma-facing tungsten. The model is a good match to the empirical correlation we published previously [Hammond et al., Acta Mater. 144, 561–578 (2018)] for small bubbles, but the current model uses no adjustable parameters. The model is likely applicable to significantly larger bubbles than the ones examined here, and its assumptions can be extended trivially to other metals and gases. We expect the model to be broadly applicable and useful in coarse-grained models of gas transport in metals.
Temperature dependence of afterglow in zirconia and its optically-stimulated luminescence by bone-through irradiation for biological temperature probe
Authors: Ohashi Masaharu , Takahashi Yoshihiro , Terakado Nobuaki , Onoue Noriko , Shinozaki Tsuyoshi , Fujiwara Takumi 
Published: February 2020

AbstractDevelopment of minimally invasive and site-selective biological temperature sensing is quite important in medical field. This study presents a novel temperature sensing technique based on afterglow and optically-stimulated luminescence (OSL). The dependence of afterglow photoluminescent intensity on the environmental temperature of zirconia (ZrO2) phosphor is examined to validate its use as a sensing probe. In addition, assuming the measurement in deep-part of human body, we have applied the information gathered from our validation to observe OSL from the ZrO2 by irradiation with near-infrared laser through a bone sample. This study demonstrates an alternative medical application of phosphor, and introduces an elemental-technology for the temperature sensing.
Determination of the concentration of impurities in GaN from photoluminescence and secondary-ion mass spectrometry
Authors: Reshchikov M. A. , Vorobiov M. , Andrieiev O. , Ding K. , Izyumskaya N. , Avrutin V. , Usikov A. , Helava H. , Makarov Yu. 
Published: February 2020

AbstractPhotoluminescence (PL) was used to estimate the concentration of carbon in GaN grown by hydride vapor phase epitaxy (HVPE). The PL data were compared with profiles of the impurities obtained from secondary ion mass spectrometry (SIMS) measurements. Comparison of PL and SIMS data has revealed that apparently high concentrations of C and O at depths up to 1 µm in SIMS profiles do not represent depth distributions of these species in the GaN matrix but are rather caused by post-growth surface contamination and knocking-in impurity species from the surface. In particular, PL analysis supplemented by reactive ion etching up to the depth of 400 nm indicates that the concentration of carbon in nitrogen sites is below 2–5 × 1015 cm−3 at any depth of GaN samples grown by HVPE. We demonstrate that PL is a very sensitive and reliable tool to determine the concentrations of impurities in the GaN matrix.