bluuebunny/crossref_metadata_2025_split huggingface dataset:
PECULIARITIES OF THE FUNCTIONAL ACTIVITY OF BLOOD EOSINOPHIL GRANULOCYTES IN PULMONARY TUBERCULOSIS
Authors: Kolobovnikova Yu. V. , Urazova O. I. , Novitskiy V. V. 
Published: June 2020

Eosinophils are polyfunctional leukocytes detected in excess in blood and in the focus of granulomatous inflammation in pulmonary TB.The research objective was to evaluate the adhesive properties as well as cytokine-secretory and antibacterial activity of blood eosinophils in pulmonary TB.The research has been conducted on eosinophils isolated from peripheral blood of 43 patients with freshly identified progressive destructive TB with and without eosinophilia. Using flow cytometry and ELISA, expression of CD9 and CD18 adhesion molecules on blood eosinophils has been studied along with the phagocyte and cytokine-secretory functions and activity of eosinophil granulocyte peroxidase.As a result of the research it has been established that in TB patients with eosinophilia the number of CD18-expressing eosinophils rises, whereas the amount of CD9+ remains within norm. Activation of the phagocyte function of blood eosinophil granulocytes is associated with the decrease in eosinophil peroxidase activity, while the increase in IL-5 and TNFα secretory reactivity is connected with oppositely directed changes in IL-2 basal secretion by eosinophils in vitro (a fall in infiltrative TB and a rise in disseminated TB).
ULTRASTRUCTURE OF SYNAPSES IN THE HIPPOCAMPUS OF RATS IN AGING
Authors: Maksimova K. Yu. , Logvinov S. V. , Stefanova N. A. 
Published: June 2020

The aim of the study was to examine age-related changes in the ultrastructure of synapses in the CA1 region of the hippocampus of Wistar rats and premature aging OXYS rats.To assess changes of the sinaptoarhitectonics photographed on 15 randomly chosen fields of view of the pyramidal layer of the CA1 region of the hippocampus with five slices at a standard magnification of 10000. It was determined the amount of mineral contacts (size of the field of view is 50 μm2) and calculated numerical density of synapses per 100 μm2. The number of contacts with asymmetric and symmetric organization sistemasubsidiaries units, perforated, hypertrophic contacts are counted.The study showed that there are significant differences in the ultrastructural organization of the synapses between the lines, already 4 months old. The numerical density of snaps in this age group OXYS rats was significantly higher than that of Wistar. Ultrastructure of synapses in the CA1 region of the hippocampus of rats at the age of 18 months more bulupodadan pathological essentiaompetencies processes and membrane prematurely senescent rats in contrast to the control line. The numerical indicator of protect contents 18-month-old rats OXYS was significantly lower than in Wistar.Identified interstrain differences in the degree of changes of synapses can testify in favor of a different sensitivity to oxidative stress in rats investigated lines.
COMPUTER SIMULATION OF GLICOPHORIN A AND 4-METHYL-2,6-DIISOBORNILFENOL INTERACTION BY AUTODOCK AND HEXSERVER PROGRAMS
Authors: Ostrikova O. I. 
Published: June 2020

4-methyl-2,6-diisobornilphenol (dibornol) – a promising drug with a hemorheological activity. Glycophorin A is one of erythrocyte membrane proteins involved in the aggregation and possibly mediating rheological effects of dibornol.Objective: to conduct a modeling of the interaction dibornol and glycophorin A by AutoDock and HexServer programs.Material and methods. We used three-dimensional models of molecules dibornol and glycophorin A. Information on the three-dimensional model of glycophorin A was received from the database RCSB Protein Data Bank – 1AFO. Modeling the three-dimensional model of a dibornol (4-methyl-2,6- diisobornilfenol) was modeling by PRODRG Server.Results. This paper presents the results of computer modeling of interaction dibornol and glycophorin A by HexServer and AutoDock programs. We used the electrostatic properties of the molecule glycophorin A, site of interaction is position chain A VAL83, chain B – ALA82, GLY83, GLY86, THR87. The energy of binding was –6.73 kcal/mol by AutoDock program, HexServer – –2.89 kcal/mol. The charge of the molecular complex dibornol-glycophorin A decreased to –4.126 (the charge of the native molecule glycophorin A – –4.003).Conclusion. Integrated use of the program AutoDock and HexServer helps significantly reduce the time and computational resources in the modeling. The study identified the amino acids that may play a key role in the interaction with dibornol glycophorin A. This study has given us reason to believe that as a result of such interaction dibornol may prevent adhesion of red blood cells.
STREPTOCOCCUS PNEUMONIAE MOLECULAR EPIDEMIOLOGICAL FEATURES IN CHILDREN OF KRASNOYARSK REGION
Authors: Protasova I. N. , Bakhareva N. V. , Peryanova O. V. , Yelistratova T. A. , Koval M. V. 
Published: June 2020

The purpose of the research was to estimate the prevalence of Streptococcus pneumoniae nasopharyngeal carriage in 250 children aged under 5 years and 215 school-aged children in Krasnoyarsk Region, and also investigate molecular epidemiological features of the strains circulating inKrasnoyarsk city and in Krasnoyarsk Region. This study is carried out for the first time in the region and provides a foundation for the choice of optimal vaccination strategy of pneumococcal disease.S. pneumoniae identification was carried out using culture method as well as PCR (revealing of cpsA gene), serotypes were determined with PCR (according to CDC protocol).The prevalence of S. pneumoniae carriage in under-five children was 51,6% with predominated pneumococcal serotypes 23F, 19F and 6В; most of these strains (up to 90%) belonged to those included in pneumococcal vaccines which are permitted for use in Russian Federation; the greatest conformity was observed in 13-valent conjugated vaccine. In school-aged children the S. pneumoniae prevalence was 12,1% with predominated pneumococcal serotypes 6В, 9VA and 15АF, most of these strains (up to 60%) belonged to those included in approved pneumococcal vaccines.Considering high level of S. pneumoniae carriage in children under 5 years in organized groups and unorganized children from families with two or more children it is necessary to carry out vaccine prevention of pneumococcal diseases with of 13-valent pneumococcal conjugated vaccine application.
THE GLUTATHIONE SYSTEM IS INVOLVED IN REGULATION OF TUMOR CELLS APOPTOSIS
Authors: Ryazantseva N. V. , Nosareva O. L. , Stepovaya Ye. A. , Zakirova Ye. V. , Naumova A. I. , Vesnina O. N. , Shakhristova Ye. V. , Orlov D. S. , Yakushina V. D. , Novitsky V. V. 
Published: June 2020

The research objective is to determine the role of the gluthatione system components in realization of the receptor pathway of Jukart tumor cell apoptosis.Apoptosis realization using FITC-labeled annexin V and propidium iodide as well as the amount of TNF R1- and Fas-presenting cells has been evaluated by flow cytofluorometry; activity caspase-3 registered a spektroflyuorimetrichesky method. The concentration of reduced and oxidated gluthatione has been determined by spectrophotometry.The material for the research was intact Jukart tumor cells and the ones incubated in the presence of a selective inhibitor of the key gluthatione synthesis enzyme – buthionine-sulfoximine.The research has shown that the gluthatione system plays an important regulatory role in activation of the receptor pathway of Jukart tumor cell apoptosis.The gluthatione system components are targets for activation of programmed cell death in tumor growth.
CLINICAL AND SOCIAL RISK FACTORS OF TARDIVE DYSKINESIA IN PATIENTS WITH SCHIZOPHRENIA DURING ANTIPSYCHOTIC TREATMENT
Authors: Kornetova Ye. G. , Semke A. V. , Dmitrieva Ye. G. , Borodyuk Yu. N. , Boyko A. S. 
Published: June 2020

The purpose of the present work was to study the clinical features and risk factors of tardive dyskinesia among the schizophrenia patients who durably receive the antipsychotic therapy. 180 of the 18 to 65 age bracket schizophrenia patients, who were treated in a residential psychiatric treatment facility, were examined with the use of the Positive and Negative Syndrome Scale (PANSS), Abnormal Involuntary Movement Scale (AIMS), and the basic chart of formalized sociodemographic and clinico-dynamic features developed at the Tomsk Mental Health Research Institute. The acquired data were processed by the Mann–Whitney U-Test and χ2. The average age of the tardive dyskinesia patients turned out to be conclusively older than that of the patients without this derangement. People who have tardive dyskinesia statistically often happen to be single in comparison with other variants of marital status. It was found out that women happen to have tardive dyskinesia more often, which allows us to see the female gender as a risk factor. The tardive dyskinesia patients had certain negative symptoms. The patients were arranged into groups according to the prepotency of symptom-complexes over the subgroups: with orofacial, thoracolumbar and combined tardive dyskinesia. The average age of the orofacial dyskinesia patients turned out to be conclusively older than that of the patients without tardive dyskinesia. The negative symptoms level in the subgroup was conclusively higher than among those without tardive dyskinesia. The average age of the thoracolumbar dyskinesia patients was conclusively older than that of the patients without tardive dyskinesia. The average age of the combined dyskinesia patients was conclusively older than the patients without the tardive dyskinesia. The patients having schizophrenia for longer than 10 years prevailed in the combined dyskinesia group. Such characteristics as education level and social status, age of when the medical problem started, dominance of the positive symptoms, duration of antipsychotic agents administration, somatic condition, use of psychoactive substances, suicidal and hetero-aggressive behaviors make no contribution to the risk of tardive dyskinesia development in the presence of schizophrenia, and they are not protective factors either.
ENDOTHELIAL DYSFUNCTION IN STABLE ANGINA AND MYOCARDIAL INFARCTION COMBINED WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE
Authors: Popova M. A. , Terentyeva N. N. , Dolgopolova D. A. , Marenina T. V. 
Published: June 2020

The research objective is to determine the state of endothelium-dependent and endothelium-independent vasodilatation in patients with coronary heart disease (CHD) associated with chronic obstructive pulmonary disease (COPD).Material and methods.In the cross-sectional study included 122 patients with CHD associated with COPD: 68 people of them are patients with stable angina without acute coronary events in history and 54 patients with acute ST segment elevation myocardial infarction (STEMI). Comparison group comprised 53 patients with stable angina and 51 patients after STEMI without concomitant COPD. Patients were included if they met the following inclusion criteria: male, age <60 years, verified forms of CHD (stable angina, STEMI), documented with COPD without exacerbation and forced expiratory volume in 1 second > 30% in the groups with CHD and COPD. Arterial endothelial function was tested with high-resolution ultrasonography: brachial artery diameter was measured at rest, after flow increase (which causes endothelium-dependent dilatation), and after administration of sublingual nitroglycerin (an endothelium-independent dilator).Results.We found that endothelial dysfunction in patients with acute and chronic forms of CHD in combination with COPD are more pronounced than in isolated CHD.Conclusion.Expressed depression functional vascular reserve in patients with CHD associated with COPD, should be taken into account when conducting individualized therapy of these patients.
HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTURE
Authors: Ratkin A. V. , Pfarger Yu. A. , Ivanov V. V. , Adekenov S. M. , Kaidash O. A. , Chuchalin V. S. , Novitsky V. V. 
Published: June 2020

Objective.Investigation of hypolipidemic effect of sesquiterpene γ-lactone Arglabin in hepatoma tissue culture (HTC).Materials and methods.In this study we’ve evaluated the effect of sesquiterpene γ-lactone Arglabin and gemfibrozil (reference drug) on the lipid content in the hepatoma tissue culture (HTC) which were incubated with a fat emulsion “Lipofundin” by fluorescent method with vital dye Nile Red. The cell viability was investigated using the MTT-test and staining by Trypan blue.Results.Cultivation of cell cultures of rat’s hepatoma cell line HTC with Arglabin and gemfibrozil in concentrations from 10 to 50 μmol and from 0.25 to 0.5 mmol, respectively, had no cytotoxic effect. HTC cell viability did not change compared with the corresponding rate in the control culture. Experimental hyperlipidemia in hepatoma culture was induced by the addition in the incubation medium of fat emulsion “Lipofundin” in a final concentration of 0.05 %. The fluorescence intensity of Nile Red in the cells was increased 4-fold (p < 0.05), which indicates a significant accumulation of lipids in the cytosol of cells. In these steady-state Arglabin and gemfibrozil at concentrations 75–100 μM and 0.25–1.0 mM, respectively, reduced the content of lipid in cells. Conclusion.In the model of hyperlipidemia induced by lipofundin, sesquiterpene γ-lactone Arglabin prevents the accumulation of lipids in the HTC cell line, as evidenced by a decrease in Nile Red fluorescence. However hypolipidemic effect of Arglabin is associated with cytotoxic effects, which is typical for anticancer drugs.
EXPRESSION OF IGFBP-6 IN COLORECTAL CANCER: THE RELATION WITH INSULIN-LIKE GROWTH FACTOR RECEPTOR, ADIPONECTIN LEVEL AND ITS RECEPTORS
Authors: Yunusova N. V. , Spirina L. V. , Afanasiev S. G. , Kondakova I. V. 
Published: June 2020

The aim of the study was to investigate the expression of insulin-like growth factor binding protein 6 (IGFBP-6) in cancer tissues in relation with clinical and morphological parameters, IGF-IR expression, serum adiponectin level and its receptors (AdipoR1, AdipoR2) in patients with colorectal cancer.Material and Methods. The study included 31 patients with colorectal cancer (T2–4N0–2M0). Serum adiponectin level, AdipoR1 and AdipoR2 expression were evaluated with ELISA. IGF-IR expression was evaluated in tumor tissue by flow cytometry. IGFBP-6 expression was evaluated with Western blotting.Results. The dependence of IGFBP-6 expression, AdipoR1, AdipoR2 on tumor invasion and lymph nodes status were revealed. There is no association IGFBP-6 expression, AdipoR1 and AdipoR2 expression and serum adiponectin level with metabolic syndrome. The revealed positive relationships between IGFBP-6 expression and AdipoR1 expression, between IGFBP-6 expression and AdipoR2 expression, between IGF-IR and AdipoR1 expression show cross-talk between IGF-IR and adiponectin/AdipoR1 pathways in colorectal carcinomas.
Computational experiment of error diffusion dithering for depth reduction in images
Authors: Rezende Costa Leonardo 
Published: June 2020

The halftone technique is a process that employs patterns formed by black and white dots to reduce the number of gray levels in an image. Due to the tendency of the human visual system to soften the distinction between points with different shades, the patterns of black and white dots produce a visual effect as if the image were composed of shades of gray and dark. This technique is quite old and is widely used in printing images in newspapers and magazines, in which only black (ink) and white (paper) levels are needed. There are several methods for generating halftone images. In this article we explore dithering with error diffusion and an analysis of different halftone techniques is presented using error diffusion to change the depth of the image. The results showed that the depth of the image changes 1/8 per channel, this halftone technique can be used to reduce an image weight, losing information but achieving good results, depending on the context.
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Basement Flood Control with Adaptive Neuro Fuzzy Inference System Using Ultrasonic Sensor
Authors: Kusumah Raden Muhamad Yuda Pradana , Abdurohman Maman , Putrada Aji Gautama 
Published: June 2020

This paper proposes a basement flood management system based on Adaptive Neuro Fuzzy Inference System (ANFIS). Basement is one of the main parts of a building that has a high potential for flooding. Therefore, the existence of a flood control system in the basement can be a solution to this threat. Water level control is the key to solving the problem. Fuzzy Inference System (FIS) has proven to be a reliable method in the control system but this method has limitations, that is, it needs to have a basis or a reference when determining the fuzzy set. When there is no basis or reference, Adaptive Neuro FIS (ANFIS) can be a solution. The Neuron aspect in ANFIS determines fuzzy sets through training data. In terms of the Internet of Things (IoT), this system uses an ultrasonic sensor, Node Red IoT platform, and Matlab Server. Then the water pump will turn on to control the water level when there is rainfall. By undergoing a comparative test with the FIS method, ANFIS provides a lower Root Mean Square Error (RMSE) and is recommended for use in basement flood management systems.
Influence of the quality of compounds on the adhesion properties of metal cord.
Authors: Khrol O. N. 
Published: June 2020

The adhesive strength of the metal cord, i. e. the strength of its adhesion to the compound, is one of the most important indicators of the quality of the metal cord. Increasing the amount of adhesive strength allow to improve the performance of finished tires.Determination of the adhesive strength value can be performed by different methods. All methods are based on extracting (pulling) metal cord from a block of vulcanized rubber and fixing the required load. Each method has its own characteristics that affect the amount of adhesive strength. Therefore, when conducting comparative tests, it is necessary to use one of the selected methods.The study of factors that affect the amount of adhesive strength is necessary to identify the causes that lead to its change.The article analyzes how the quality of the used compound affects the adhesive strength. Tests with the use of compounds with various defects were considered and carried out; probable factors leading to deviations in the adhesion strength indicators were determined.
Decrease of Spermatogonia, Spermatocytes and Sertoli Cell Counts Male Mice (Mus musculus) Induced by Ethylene Glycol
Authors: Latief Nuraini 
Published: June 2020

The experiment was conducted to study the effect of ethylene glycol (EG) on disturbance of spermatogenesis process of the male mice. Sixteen mice at the age of 2 months were randomly into 4 groups of 4 each. The mice of control group were only given drinking water without EG. Group KPI, KP2, and KP3 were given EG 5, 10, and 15 ml/liter drinking water, respectively. On day 31 of post treatment, all mice were killed for their testis. The testis were collected and processed for microscopic examination and haematoxylin-eosin staining. The result showed that EG given orally for 30 days on male mice have negative effect on the process of spermatogenesis as the numbers of spermatogonia, spermatocyte and Sertoli cells decreased significantly (P0.01)
PENGARUH LAMA MATURASI DAN LAMA INKUBASI FERTILISASI TERHADAP ANGKA FERTILITAS OOSIT SAPI PERANAKAN ONGOLE SECARA IN VITRO
Authors: Kusindarta Dwi Liliek 
Published: June 2020

Penelitian ini dilakukan untuk mengetahui pengaruh lama maturasi dan lama inkubasi fertilisasi terhadap angka fertilitas oosit sapi peranakan Ongole (PO) secara in vitro. Oosit diaspirasi dari folikel ovarium yang berdiameter 2-6 mm. Oosit dirandom untuk penelitian menggunakan rancangan blok acak sempurna (RCBD), dengan lama maturasi 20, 22 dan 24 jam sebagai blok dan lama inkubasi fertilisasi 4, 5 dan 6 jam sebagai perlakuan. Hasil penelitian menunjukkan angka fertilitas oosit sapi PO dengan lama maturasi 20 jam dan lama inkubasi fertilisasi 4, 5 dan 6 jam masing-masing adalah 48,86%+3,88, 48,08%+2,22 dan 51,96%+ 2,27. Angka fertilitas oosit sapi PO dengan lama maturasi 22 jam dan lama inkubasi fertilisasi 4, 5 dan 6 jam masing-masing adalah 50,14%+4,18, 51,14%+4,35 dan 52,27%+2,26. Angka fertilitas oosit sapi PO dengan lama maturasi 24 jam dan lama inkubasi fertilisasi 4, 5 dan 6 jam masingmasing adalah 51,21%+4,49, 53,49%+2,43 dan 50,96%+4,18. Tidak ada perbedaan yang nyata (P0,05) angka fertilitas antara lama maturasi 20, 22 dan 24 jam dan tidak ada perbedaan yang nyata (P0,05) angka fertilitas antara lama inkubasi fertilisasi 4, 5 dan 6 jam.
KONSENTRASI PROTEIN DAN PENENTUAN BERAT MOLEKUL EKSKRETORI/SEKRETORI L3 Ascaridia galli
Authors: D Darmawi , Balqis Ummu , Tiuria Risa , Soejoedono Retno D , Pasaribu Fachriyan H 
Published: June 2020

Penelitian ini bertujuan menentukan konsentrasi dan berat molekul protein ekskretori/sekretori larva (L3) Ascaridia galli (A. galli). Larva L3 diperoleh dari usus halus 100 ayam tujuh hari setelah pemberian dosis 6000 L2 melalui esofagus ayam. Sebanyak 5–10 L3 dikultur secara in vitro dalam setiap ml medium Rosswell Park Memorial Institute (RPMI 1640), pH 6,8, tanpa merah fenol dalam inkubator pada temperatur 37 0C dan 5% CO2 selama 3 hari. Ke dalam medium ditambahkan 100 unit ml-1 penisilin G, 100 µg ml-1 streptomisin, 5 µg ml-1 gentamisin dan 0,25 µg ml-1 kanamisin. Ekskretori/sekretori dipreparasi dari produk metabolisme L3 yang dilepaskan ke dalam medium kultur. Untuk mendapatkan protein ekskretori/sekretori, medium kultur dipekatkan dengan vivaspin 30.000 MWCO, dan kuantitas protein dihitung dengan metode Bradford. Berat molekul protein ekskretori/sekretori divisualisasikan dengan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Hasil penelitian menunjukkan bahwa konsentrasi protein ekskretori/sekretori adalah 0,595 mg/ml dengan berat molekul 28 kDa.
Pathogenicity of Eimeria mivati Inoculation in Broiler Chicken
Authors: Hasan M 
Published: June 2020

The propagation of E. mivati local strain was used single cell inoculation. The objective of this study was to determine the pathogenicity of Eimeria mivati inoculation in broiler chicken by measuring the growth rate, oocysts production and levels of changes in small intestine. The chickens were devided into two age groups of infection, three and teen days old. Each group was received 1x104, 1x105 and 1x106 oocysts/chick. The chichen were raised in wire cages and sampled accordingly. Data collected was analyzed using analysis of variance. A large number of oocysts production, intestinal villi lesions and depression of growth rate were seen after infection. Most of the peak production of oocysts were accured after the 9th day of infection. However, after the peak production of oocysts, oocysts production decreased extremely. The choice of parasitic development were in the surface epithelial cells of the villi and the region dominantly effected was the third anterior of small intestine. The result revealed no significant differences regarding photogenicity findings (P0.05) between chicken aged three and ten days after infected with E. mivati.
The Development of Ascaridia galli Infective Eggs by In Vitro Culture
Authors: Balqis Ummu , D Darmawi , Hambal Muhammad , Tiuria Risa 
Published: June 2020

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli, embrionated eggs