bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decreased hsa_circ_0001944 expression is associated with improved bladder cancer prognoses
Authors: Jin Mingming , Lu Shengjie , Wu Yue , Yang Chen , Shi Chunzi , Wang Yanqiu , Huang Gang 
Published: August 2020

Abstract
Background: Bladder cancer (BC) is a common genitourinary malignancy worldwide. Circular RNAs (circRNAs) participate in cancer development, including BC; thus, the roles of circRNAs in this process have attracted significant attention. Methods: In this study, high-throughput sequencing was used to analyze circRNA expression profiles in BC tissues. We performed RT-qPCR to determine hsa_circ_0001944 expression in BC tissues. We used fluorescence in situ hybridization (FISH) to detect hsa_circ_0001944 expression and hsa_circ_0001944 subcellular localization in BC tissues. hsa_circ_0001944 expression in BC cells was selectively regulated. We employed CCK8, transwell, and wound healing assays to monitor cell proliferation, invasion, and migration, respectively. We employed the dual-luciferase reporter and RNA pulldown assays to verify the relationships among hsa_circ_0001944, miR-548, and PROK2. We examined the effects of hsa_circ_0001944 on BC cell metastasis and proliferation in vivo using a subcutaneous xenograft model and an intravenous tail injection model in nude mice. Results: The results showed that hsa_circ_0001944 expression was significantly increased in BC samples. Furthermore, high hsa_circ_0001944 expression predicted unfavorable prognoses in BC. Functional assays validated that downregulating hsa_circ_0001944 decreased BC invasion and proliferation in vivo and in vitro. Further studies showed that hsa_circ_0001944 expression promoted BC progression via sponging miR-548 and enhancing PROK2 expression. Luciferase reporter experiments validated the interactions between hsa_circ_0001944, miR-548, and PROK2. This study also found that downregulating miR-548 or overexpressing PROK2 restored BC cell invasion and proliferation after silencing hsa_circ_0001944. Conclusions: Taken together, we found that hsa_circ_0001944 is a tumor-promoting circRNA in BC that functions as a competing endogenous RNA to regulate PROK2 expression via sponging miR-548.
Identification of collagen genes related to immune infiltration and epithelial-mesenchymal transition in glioma
Authors: Yin Wen , Tan Jun , Xin Zhaoqi , Zhou Quanwei , Cao Yudong , Wu Zhaoping , Lei Wang , Jiang Xingjun , Ren Caiping , Tang Guihua 
Published: August 2020

Abstract
Background: Gliomas account for the majority of fatal primary brain tumors, and there is much room for research in the underlying pathogenesis, the multistep progression of glioma, and how to improve survival. In our study, we aimed to identify potential biomarkers or therapeutic targets of glioma and study the mechanism underlying the tumor progression. Methods: We downloaded the microarray datasets (GSE43378 and GSE7696) from the Gene Expression Omnibus (GEO) database. Then, we used weighted gene co-expression network analysis (WGCNA) to screen potential biomarkers or therapeutic targets related to the tumor progression. ESTIMATE (Estimation of STromal and Immune cells in MAlignant Tumors using Expression data) algorithm and TIMER (Tumor Immune Estimation Resource) database were used to analyze the correlation between the selected genes and the tumor microenvironment. Real-time reverse transcription polymerase chain reaction was used to measure the selected gene. Transwell and wound healing assay were used to measure the cell migration and invasion capacity.Results: We identified specific module genes that were positively correlated with the WHO grade but negatively correlated with OS of glioma. Importantly, we identified that 6 collagen genes (COL1A1, COL1A2, COL3A1, COL4A1, COL4A2, and COL5A2) could regulate the immunosuppressive microenvironment of glioma. Moreover, we found that these collagen genes were significantly involved in the epithelial-mesenchymal transition (EMT) process of glioma. Finally, taking COL5A2 as a further research object, the results showed that knockdown of COL5A2 significantly inhibited the migration and invasion of SHG44 and A172 cells. Conclusions: In summary, our study demonstrated that collagen genes play an important role in regulating the immunosuppressive microenvironment and EMT process of glioma and could serve as potential therapeutic targets for glioma management.
Incidence and Risk Factors of postoperative Dysphagia following Cervical Spine Surgery
Authors: Yu Jie , Tao Xiaohui 
Published: August 2020

Abstract
Background. Dysphagia is a known complication of anterior cervical spine surgery and may be prolonged or occasionally serious. However, the association between posterior cervical spine surgery and the development of dysphagia is rare discussed. Furthermore, the risk factors of postoperative dysphagia are not well understood.Methods. Three hundred and fifty four patients were reviewed in this study, including 172 patients undergoing AC procedure, and 182 patients with PC procedure between June 2007 and May 2012. All patients were given the same postoperative management. The presence and severity of dysphagia were reported during the telephone interviews performed at 1, 3, 6, and 12 months after the procedure. The incidence and risk factors of dysphagia after cervical spine surgery were studied. Results. Of all 354 patients, 12.8% AC and 9.4% PC patients reported dysphagia at early postoperative assessment. The overall incidence for dysphagia at 1, 3, 6 and 12 months were decreased to 9.3%, 7.0%, 4.6% and 3.5% in AC group, and 6.0%, 4.4%, 2.2% and 1.6% in PC group, respectively. Long term use of Neck Collar after surgery may lead to postoperative dysphagia. Other risk factors such as age, gender, BMI, operative time, blood loss, procedure type, revision surgery and operative levels did not signiﬁcantly influence the incidence of postoperative dysphagia.Conclusions. Postoperative dysphagia is common after anterior and posterior cervical spine surgery. The incidence and severity of dysphagia both decrease over time. Long term use of neck collar after surgery is related of postoperative dysphagia. The neck collar should be worn no longer than four weeks after surgery. Age, gender, BMI, operative time, blood loss, procedure type, revision surgery and operative levels are not risk factors of postoperative dysphagia.
Parthenolide inhibits the growth of non-small cell lung cancer by targeting epidermal growth factor receptor
Authors: Li Xiaoling , Huang Riming , Li Mingyue , Zhu Zheng , Chen Zhiyan , Cui Liao , Luo Hui , Luo Lianxiang 
Published: August 2020

Abstract
Background: EGFR tyrosine kinase inhibitors (TKIs) have been developed for the treatment of EGFR mutated NSCLC. Parthenolide, a natural product of parthenolide, which belongs to the sesquiterpene lactone family and has a variety of biological and therapeutic activities, including anti-cancer effects. However, its effect on non-small cell lung cancer is little known. Methods: The CCK‑8 assay and colony formation assays were used to assess cell viability. Flow cytometry was used to measure the cell apoptosis. In silico molecular docking was used to evaluate the binding of parthenolide to EGFR. Network pharmacology analysis was was used to evaluate the key gene of parthenolide target NSCLC. Western blotting was used to evaluate the key proteins involved apoptosis and EGFR signalling. The effect of parthenolide treatment in vivo was determined by using a xenograft mouse model.Results: In this study, parthenolide could induce apoptosis and growth inhibition in the EGFR mutated lung cancer cells. Parthenolide also reduces the phosphorylation of EGFR as well as its downstream signaling pathways MAPK/ERK and PI3K/Akt. Molecular docking analysis of EGFR binding site with parthenolide show that the anti-cancer effect of parthenolide against NSCLC is mediated by a strong binding to EGFR. Network pharmacology analysis show parthenolide suppresses NSCLC via inhibition of EGFR expression. In addition, parthenolide inhibits the growth of H1975 xenografts in nude mice, which is associated with the inhibition of the EGFR signaling pathway. Conclusions: Taken together, these results demonstrate effective inhibition of parthenolide in NSCLC cell growth by targeting EGFR through downregulation of ERK and AKT expression, which could be promisingly used for patients carrying the EGFR mutation.
Impact of fibrinogen level on the prognosis of patients with traumatic brain injury: A single-center analysis of 2570 patients
Authors: Lv Ke , Yuan Qiang , Fu Pengfei , Wu Gang , Wu Xing , Du Zhuoying , Yu Jian , Li Zhiqi , Hu Jin 
Published: August 2020

Abstract
Background: Fibrinogen may play an important role in the survival of trauma patients; however, its role in traumatic brain injury (TBI) and its correlation with disease prognosis remain poorly understood. The aims of this study were to determine the incidence of TBI-associated hypofibrinogenemia in patients with TBI and to evaluate the prognostic value of fibrinogen level with respect to mortality and clinical outcomes. Methods: A total of 2570 consecutive TBI patients were retrospectively studied. Prognostic evaluations were determined using the Glasgow Outcome Score (GOS) assessment 3 months after injury. The shape of the relationship between fibrinogen level and mortality or outcome was examined using cubic spline functions. Logistic regression analyses were conducted to identify the association between fibrinogen level and 3-month functional outcomes.Results: Fibrinogen concentrations < 2 g/L were observed in 992 (38.6%) patients at the time of admission. Multivariate analyses showed that for patients with fibrinogen levels < 2.0 g/L, those levels were an independent prognostic factor for 3-month mortality (odds ratio [OR], 0.91; 95% confidence interval [CI], 0.89–0.93; P < .001). By contrast, for patients with fibrinogen levels < 2.5 g/L, the levels were an independent prognostic factor for favorable outcomes at 3 months (OR, 1.654; 95% CI, 1.186–2.306; P = .003). Similar results were also seen for patients with fibrinogen levels > 3.0 g/L, with the levels being an independent prognostic factor for favorable outcomes at 3 months (OR, 0.771; 95% CI, 0.607–0.979; P = .033). Conclusions: Fibrinogen is an independent prognostic factor for clinical outcomes in TBI patients. Maintaining the level of fibrinogen between 2.5 and 3 g/L may improve clinical outcomes in patients with TBI.
Cardiac metastases from primary myxoid liposarcoma of the thigh: A case report
Authors: Ikuta Kunihiro , Sakai Tomohisa , Koike Hiroshi , Okada Tohru , Imagama Shiro , Nishida Yoshihiro 
Published: August 2020

Abstract
BackgroundMyxoid liposarcoma is well known to have an unusual proclivity for extrapulmonary metastasis. However, cardiac metastasis of myxoid liposarcoma is very rare, even in patients with advanced disease.Case presentationA 40-year-old man was diagnosed with myxoid liposarcoma of the right thigh and treated with wide resection. Two years after the surgery, a low-density area in the left ventricle was found on follow-up chest computed tomography, and was suspected of being metastatic disease. He underwent surgical treatment, and the lesion was pathologically confirmed as metastasis of myxoid liposarcoma. Fifteen months later, he complained of slight dyspnea and developed metastatic disease in the right atrium. He was treated with surgical excision, followed by radiotherapy. Although there was no recurrence in the heart since the second cardiac metastasectomy, multiple metastases occurred in the abdominal cavity, lungs, and muscles. He finally died of the disease two years after the second cardiac metastasectomy.ConclusionWe experienced a case of primary myxoid liposarcoma in the thigh, accompanied by ectopic and metachronous cardiac metastases. Although this condition is rare, we should follow-up patients with myxoid liposarcoma, considering the possibility of cardiac metastasis.
Perceived Stress and Its Impact on Health Behavior of Chinese Residents during the Epidemic of COVID-19: An Internet Survey
Authors: Li Yuerong , Yao Lili , Luo Yetao , Yuan Feng , Yan Lupei 
Published: August 2020

Abstract
Background: The outbreak of 2019 novel coronavirus disease(COVID-19) caused severe respiratory illnesses, which might be resulted from air-borne droplets or direct contact, posing a great threat to human life. Meanwhile, the epidemic might trigger excessive stresses and produce terrible impacts on mental health even health behaviors, especially of the vulnerable individuals. So this study paved the way for psychological interventions focused on vulnerable groups during the COVID-19 epidemic.Methods: An internet survey was conducted among 2,449 residents in 20 Provinces of China, collecting the data about the perceived stress, the cognition of COVID-19 and the health behaviors. SAS9.4 was used to analyze the relationship between health behaviors and perceived stress, and logistic regression was used to explore the influencing factors of health risk stress.Results: The perceived stress score was 22.25±7.2(total56), and the incidence of health risk stress was 39.89% (977/2449). Perceived stress increased, while the frequency of health behaviors decreased, such as washing hands and wearing masks. Age, cognition of susceptibility to COVID-19, life-threatening level, cognition of the importance of home isolation, and cognition of the difference between common cold and COVID-19 were positively related to the occurrence of health risk stress.Conclusions: A negative correlation was found between health behaviors and perceived stress.Therefore, it is of great significance to improve residents’ knowledge of the COVID-19, to provide good social support and psychological interventions for those how are experiencing health risk stress and to promote their health behaviors.
PHOENIX: A new framework for applying psychological theories to the adoption of innovations by healthcare professionals
Authors: Fahy Nick , Greenhalgh Trish , Shaw Sara 
Published: August 2020

Abstract
Background: Although implementation science has borrowed concepts from psychology to explain how individuals think and act in relation to innovation adoption, the use of psychology has been limited and not well adapted to the aims and obligations of professionals. We aimed to enrich implementation science with a broader set of psychological theories relevant for professional behaviour, drawing particularly on the diffusion of innovations and stages of change models.Methods : The study had two parallel components. The first was a hermeneutic review of how psychological theory has and could be used to understand innovation adoption by health professionals. The second component was an empirical case study of the implementation of guidance on routine HIV screening in three sites across two London hospitals. Primary data sources were synthesised and combined with emerging secondary research findings, each feeding into the other. Analysis focused empirically on how the stages of the diffusion of innovations adoption model (prior conditions, knowledge, persuasion, decision, implementation, confirmation) could be enriched by psychological theories. Results: The hermeneutic review identified at least 20 relevant psychological theories from social, organisational, developmental and sports psychology; many of these were not single theories but contributions from domains of evolving research, making this an underestimate of potentially relevant theory. We developed a model (PHOENIX) to encapsulate how psychological theory can help illuminate each stage of adoption from diffusion of innovations theory:· Person and History (prior conditions stage) – individuals’ characteristics and past experiences;· Overload avoidance (knowledge stage) – filtering strategies when faced with overwhelming amounts of new knowledge;· Evolving attitudes (persuasion stage) – a social process of collectively emerging views within a team;· Networked decisions (decision stage) – decisions at two levels of the department combined with individual judgements;· Implementation support (implementation stage) to turn an intention to act into actual action, with external supports valuable to reduce the cognitive effort required;· uneXceptional (confirmation stage) – sustained effort to turn the new behaviour from a conscious innovation to a habit. Conclusions: The PHOENIX model, if affirmed in further studies, has potential to enrich the study of innovation adoption by healthcare staff in organisational and professional contexts.
Comparing Patient Experiences Before and After Re-Organizing Inpatient Pain Care: A Before and After Study
Authors: Germossa Gugsa Nemera , Hellesø Ragnhild , Strømseng Sjetne Ingeborg 
Published: August 2020

Abstract
Background: Inadequate pain care is a significant problem for patients during hospitalization, particularly in Ethiopia. Evidence has shown that work process re-organization improves patient-reported outcomes.This study aimed to compare patients’ experiences before and after re-organizing inpatient pain care Method: A quasi-experimental design on a separate sample was conducted between October 1, 2016, and June 15, 2017. Participants of the study were patients admitted to the four inpatient units (medical, surgical, maternity, and gynaecology) of Jimma Medical Centre. Seven hundred eighty-two (Survey 1: N=256; Survey 2: N=259; Survey 3: N= 267) patients out of 845 patients invited to participate (92.5%) in three consecutive surveys were included in the analysis. A patient survey questionnaire adapted from a Brief Pain Inventory, the American Pain Society Patient Outcome Questionnaire, and Pain Treatment Satisfaction Scale was used. Data were mainly analysed using ANOVA. A significant difference between samples was declared at a P-value of less than 0.05.Results: Patients reported experiences with pain care significantly increased after re-organizing in-hospital pain care. More specifically, although there was a growing misfit between information provided and adequacy, staff responsiveness within 30 minutes, and patient satisfaction with pain management significantly increased. These increments were statistically significant with p < 0.05. Conclusion: Re-organizing in-hospital pain care improves patient-reported pain care experiences by changing both nurses’ technical capacity and the work process at inpatients’ units
Open Versus Arthroscopic Release for Lateral Patellar Compression Syndrome: A Randomized Controlled Trial
Authors: Hamawandi Sherwan , Amin Hazhar , Al-Humairi Ameer Kadhim 
Published: August 2020

Abstract
Background: Lateral patellar compression syndrome is one of the causes of anterior knee pain in young adults and resulted from tight lateral patellar retinaculum, which can be treated by surgical release of tight retinaculum after failure of conservative measures. Surgical release can be done by open or arthroscopic procedures. There was no randomized control trial to compare between these two procedures in considering of functional outcome and complications. The aim of our study is to compare between open and arthroscopic release of lateral patellar compression syndrome in relation of functional outcome, time of surgical procedure, length of hospital stays, intraoperative and postoperative complications as bleeding, infection, recurrence and patellar instability with 2 years of follow up. Methods: 80 patients, age (21-49 years), were divided randomly into 2 groups (A and B). Group A (40 patients) were treated with open release after diagnostic arthroscopy. Group B (40 patients) were treated by arthroscopic release. All these patients are diagnosed as lateral patellar compression syndrome depending on clinical features, MRI and diagnostic arthroscopy and they had failure of conservative measures of quadriceps strengthening and analgesics for 6 months. All patients were assessed by Lysholm knee scoring scale before surgery and at periods of 2 weeks, 6 weeks, 6 months, 12 months and 24 months after surgery. Results: There is significant difference in functional outcome, measured by Lysholm knee scoring scale, between preoperative and postoperative assessment periods in both groups (P<0.001). There is significantly better functional outcome at 2 years of follow up with the arthroscopic release (P=0.018). There is no recurrence in both groups, but there were 4 patients develop medial patellar instability in the group that was treated by open release. There are 2 patients develop hemarthrosis in arthroscopic group and one patient develop superficial wound infection in the group of open release. There is no significant difference in the postoperative complications between two groups. Conclusion: Both open and arthroscopic lateral release for patients with lateral patellar compression syndrome (without instability, limb malalignment or dysplasia) are effective surgical procedures but arthroscopic release can achieve better functional outcome than open release with less risk of development of post-operative patellar instability.Trial registration: NCT, NCT04130412. Registered 15 October 2019 -Retrospectively registered, https://www.clinicaltrials.gov/NCT04130412
The Validation of Conventional Non-Invasive Fibrosis Scoring Systems in Patients with Metabolic Associated Fatty Liver Disease
Authors: Wu Yinlian , Kumar Rahul , Wang Mingfang , Singh Medha , Huang Jiaofeng , Zhu Yueyong , Lin Su 
Published: August 2020

Abstract
Background：Non-invasive fibrosis scores are not yet validated in the newly defined metabolic associated fatty liver disease (MAFLD). This study evaluated the diagnostic performance of four non-invasive scores including AST to platelet ratio index (APRI), fibrosis-4 index (FIB-4), BMI, AST/ALT ratio, and diabetes score (BARD), and NAFLD fibrosis score(NFS) in patients with MAFLD.Methods: Consecutive patients with histologically-confirmed MAFLD were included. The discrimination ability of different non-invasive scores was compared.Results: A total of 417 patients were included, 156 (37.4%) of them had advanced fibrosis (METAVIR ≥F3). The area under receiver operating characteristic curve (AUROC) of FIB-4, NFS, APRI and BARD for predicting advanced fibrosis were 0.736, 0.724, 0.671 and 0.609 respectively. The AUROC between FIB-4 and NFS were similar (P=0.523), while the difference between FIB-4 and APRI (P=0.001) and FIB-4 and BARD (P<0.001) was statistically significant. The best thresholds of FIB-4，NFS，APRI and BARD for diagnosis of advanced fibrosis in MAFLD were 1.05, -2.1, 0.42 and 2. A subgroup analysis showed that FIB-4, APRI and NFS performed worse in pure MAFLD than HBV-MAFLD group.Conclusions: APRI and BARD score do not perform well in MAFLD. The FIB-4 and NFS could be more useful but new threshold is needed. Novel non-invasive scoring system for fibrosis is required for MAFLD.