bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causal effects of serum levels of n-3 or n-6 polyunsaturated fatty acids on coronary artery disease: Mendelian randomization study
Authors: Park Sehoon , Lee Soojin , Kim Yaerim , Lee Yeonhee , Kang Min Woo , Kim Kwangsoo , Kim Yong Chul , Han Seung Seok , Lee Hajeong , Lee Jung Pyo , Joo Kwon Wook , Lim Chun Soo , Kim Yon Su , Kim Dong Ki 
Published: October 2020

AbstractBackgroundAdditional studies on the causal effects of 3-n and 6-n polyunsaturated fatty acids (PUFAs) on the risk of coronary artery disease (CAD) are warranted.MethodsThis Mendelian randomization (MR) study utilized a genetic instrument developed from previous genome-wide association studies for various serum 3-n and 6-n PUFA levels. First, we calculated the allele scores for genetic predisposition of PUFAs in individuals of European ancestry in the UK Biobank data (N=337,129). The allele score-based MR was obtained by regressing the allele scores to CAD risks. Second, summary-level MR was performed with the CARDIoGRAMplusC4D data for CAD (N=184,305). The inverse variance-weighted or Wald ratio method was the main analysis for the summary-level MR, and when multiple single nucleotide polymorphisms were utilized (e.g., linoleic acid), MR-Egger and weighted median methods were implemented as sensitivity analyses.ResultsHigher genetically predicted eicosapentaenoic acid and dihomo-gamma-linolenic acid levels were significantly associated with a lower risk of CAD both in the allele-score-based and summary-level MR analyses. Higher allele scores for linoleic acid level were significantly associated with lower CAD risks, and in the summary-level MR, the causal estimates by the MR-Egger and weighted median methods also indicated that higher linoleic acid levels cause a lower risk of CAD. Arachidonic acid was the 6-n PUFA that showed significant causal estimates for a higher risk of CAD. Higher docosapentaenoic acid and adrenic acid levels showed inconsistent findings in the MR analysis results.ConclusionsThis study supports the causal effects of certain 3-n and 6-n PUFA types on the risk of CAD.
Single cell resolution of SARS-CoV-2 tropism, antiviral responses, and susceptibility to therapies in primary human airway epithelium
Authors: Fiege Jessica K. , Thiede Joshua M. , Nanda Hezkiel , Matchett William E. , Moore Patrick J. , Montanari Noe Rico , Thielen Beth K. , Daniel Jerry , Stanley Emma , Hunter Ryan C. , Menachery Vineet D. , Shen Steven S. , Bold Tyler D. , Langlois Ryan A. 
Published: October 2020

AbstractThe human airway epithelium is the initial site of SARS-CoV-2 infection. We used flow cytometry and single cell RNA-sequencing to understand how the heterogeneity of this diverse cell population contributes to elements of viral tropism and pathogenesis, antiviral immunity, and treatment response to remdesivir. We found that, while a variety of epithelial cell types are susceptible to infection, ciliated cells are the predominant cell target of SARS-CoV-2. The host protease TMPRSS2 was required for infection of these cells. Importantly, remdesivir treatment effectively inhibited viral replication across cell types, and blunted hyperinflammatory responses. Induction of interferon responses within infected cells was rare and there was significant heterogeneity in the antiviral gene signatures, varying with the burden of infection in each cell. We also found that heavily infected secretory cells expressed abundant IL-6, a potential mediator of COVID-19 pathogenesis.
A novel viral protein translation mechanism reveals mitochondria as a target for antiviral drug development
Authors: Cheng Zhenguo , Zhang Danhua , Chen Jingfei , Wu Yifan , Liu XiaoWen , Si Lingling , Zhang Zhe , Zhang Na , Zhang Zhongxian , Liu Wei , Liu Hong , Zhang Lirong , Song Lijie , Dunmall Louisa S Chard , Dong Jianzeng , Lemoine Nicholas R , Wang Yaohe 
Published: October 2020

AbstractThe ongoing Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) pandemic has acutely highlighted the need to identify new treatment strategies for viral infections. Here we present a pivotal molecular mechanism of viral protein translation that relies on the mitochondrial translation machinery. We found that rare codons such as Leu-TTA are highly enriched in many viruses, including SARS-CoV-2, and these codons are essential for the regulation of viral protein expression. SARS-CoV-2 controls the translation of its spike gene by hijacking host mitochondria through 5’ leader and 3’UTR sequences that contain mitochondrial localization signals and activate the EGR1 pathway. Mitochondrial-targeted drugs such as lonidamine and polydatin significantly repress rare codon-driven gene expression and viral replication. This study identifies an unreported viral protein translation mechanism and opens up a novel avenue for developing antiviral drugs.One Sentence SummaryMitochondria are a potential target for antiviral therapy
Targeting of CXCR4 by the naturally occurring CXCR4 antagonist EPI-X 4 in Waldenström’s Macroglobulinemia
Authors: Kaiser Lisa M. , Harms Mirja , Sauter Daniel , Rawat Vijay PS , Glitscher Mirco , Hildt Eberhard , Tews Daniel , Hunter Zachary , Münch Jan , Buske Christian 
Published: October 2020

AbstractCXCR4 expression and downstream signaling have been identified as key factors in malignant hematopoiesis. Thus, up to 40% of all patients with Waldenström’s Macroglobulinemia (WM) carry an activating mutation of CXCR4 that leads to a more aggressive clinical course and inferior outcome upon treatment with the Bruton’s tyrosine kinase inhibitor ibrutinib. Nevertheless, little is known about physiological mechanisms counteracting CXCR4 signaling in hematopoietic neoplasms. Recently, the endogenous human peptide EPI-X4 was identified as a natural CXCR4 antagonist that effectively blocks CXCL12-mediated receptor internalization and suppresses the migration and invasion of cancer cells towards a CXCL12 gradient. Here, we demonstrate that EPI-X4 efficiently impairs growth of WM cellsin vitroandin vivoand blocks their migration towards CXCL12. The CXCR4 inhibitory activity of EPI-X4 is accompanied by decreased expression of genes involved in MAPK signaling and energy metabolism. Notably, the anti-WM activity of EPI-X4 could be further augmented by the rational design of EPI-X4 derivatives showing higher binding affinity to CXCR4. In summary, these data demonstrate that a naturally occurring anti-CXCR4 peptide is able to interfere with WM growth, and that optimized derivatives of EPI-X4 may represent a promising approach in suppressing growth promoting CXCR4 signaling in WM.
Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts
Authors: Kepser Lara-Jane , Hinojosa Laura Soto , Macchi Chiara , Ruscica Massimiliano , Marcello Elena , Culmsee Carsten , Grosse Robert , Rust Marco B. 
Published: October 2020

AbstractRecent studies identified cyclase-associated proteins (CAPs) as important regulators of actin dynamics that control assembly and disassembly of actin filaments (F-actin). While these studies significantly advanced our knowledge of their molecular functions, the physiological relevance of CAPs largely remained elusive. Gene targeting in mice implicated CAP2 in heart physiology and skeletal muscle development. Heart defects in CAP2 mutant mice were associated with altered activity of serum response factor (SRF), a transcription factor involved in multiple biological processes including heart function, but also skeletal muscle development. By exploiting mouse embryonic fibroblasts (MEFs) from CAP2 mutant mice, we aimed at deciphering the CAP2-dependent mechanism relevant for SRF activity. Reporter assays and mRNA quantification by qPCR revealed reduced SRF-dependent gene expression in mutant MEFs. Reduced SRF activity was associated with moderately increased G-actin levels and reduced nuclear levels of MRTF-A, a transcriptional SRF coactivator that is shuttled out of the nucleus and, hence, inhibited upon G-actin binding. Moreover, pharmacological actin manipulation restored MRTF-A distribution in mutant MEFs. Our data are in line with a model in which CAP2 controls the MRTF-SRF pathway in an actin-dependent manner. While MRTF-A localization and SRF activity was impaired under basal conditions, serum stimulation induced nuclear MRTF-A translocation and SRF activity in mutant MEFs similar to controls. In summary, our data revealed that in MEFs CAP2 controls basal MRTF-A localization and SRF activity, while it was dispensable for serum-induced nuclear MRTF-A translocation and SRF stimulation.
ICT as a solution for the revitalization of public open space in private developments
Authors: Jian Izzy Yi , Chan Edwin H.W. , Yao Terry Ye Peng 
Published: October 2020

PurposePOSPD, as supplementation of public open spaces (POS), has become a common policy to moderate the intensification of urbanization. However, some access restrictions, both physical and information-wise, were deliberately designed by private developers to reserve the POSPD for their own gains, which further hampers POSPD’s publicness and leads to their failure to bear social responsibilities.Design/methodology/approachBy analyzing the current situation of the availability of public open space in private developments (POSPD) from the perspective of information justice, this research aims at proposing a policy framework for an “accessible and interactive platform” which advocates promoting informational justice by integrating public participation into the establishment of an interaction loop to promote the revitalization of POSPD. The methodology includes the review of previous solutions and platforms, the establishment of a POSPD database and geographic information system (GIS) analysis.FindingsThe POSPD in Hong Kong are unevenly distributed physically while the information about them is injustice and inadequate. Understanding the existing informational injustice associated with POSPD and revitalizing the stock spaces is timely and vital. Using the user-generated data from volunteers as the information flow, the proposed responsive POSPD platform will provide continuous positive feedback for policy improvement to help realize the collaborative management and sustainable development of the POS.Originality/valueMaking use of information and communication technology (ICT) to extend the “public” to the “internet-based”, the proposed framework regards the exploitation of ICT to enhance information justice as a novel way to revitalize POSPD. It involves collaborative operation among citizen participation and official POS management.
The asymmetric impact of institutional ownership on firm performance: panel smooth transition regression model
Authors: Daryaei Abbas Ali , Fattahi Yasin 
Published: October 2020

PurposeThis study aims to test the asymmetric impact of institutional ownership on firm performance. This study does it through an examination of the hypotheses of efficient monitoring and convergence of interests from the Tehran Stock Exchange (TSE).Design/methodology/approachUsing a panel smooth transition regression model, as a new econometric technique, this paper examined the data to explore the asymmetric impact of institutional ownership on firm performance. With regard to 177 firms for the period 2009 to 2018 from TSE. Performance proxies are returned on asset (ROA), return on equity (ROE) and Tobins’ Q.FindingsThe empirical for three performance proxies results strongly rejected the null hypothesis of linearity and the test for no remaining nonlinearity indicated a model with one transition function and one threshold parameters. The first regime (levels of institutional ownership below 28.5% and 43.5% for ROA and Tobins’ Q) showed that performance increases with institutional ownership while the trend was reversed in the second regime (levels of institutional ownership above 28.5% and 43.5% for ROA and Tobins’ Q percent). Also, institutional shareholders percent between 4.2 and 14.1 explain the positive relationship between institutional shareholders and ROE.Originality/valueFurthermore, the findings of this study suggest that the application of institutional ownership theories calls for more inquiry.
Mentoring reality: from concepts and theory to real expertise and the mentor’s point of view
Authors: Zentgraf Laura Lidija 
Published: October 2020

Purpose
The purpose of this paper is to illustrate the reality of mentoring in higher education from the mentor’s point of view. The goal is to elaborate best practice of mentoring on issues such as advanced training, professionalization, experience exchange, networking, de facto tasks and levels of interaction with mentees.

Design/methodology/approach
This paper examines the gap between theoretical mentoring methods, for example, with regard to timely planning and the reality of being a mentor. Data were generated by qualitative interviews of up to 45 min with mentors with several years of experience. The qualitative findings will help interested people to get a feel for what it means to be a mentor.

Findings
The findings showed that each mentor found his or her individual way of mentoring and that each was eager to personally improve. The paper shows influencing factors beyond project planning, such as emotional involvement, dealing with student denial and the time it actually takes to be a good mentor beyond office hours.

Research limitations/implications
As matriculation numbers must not be documented due to German data protection law, the de facto effectiveness of mentoring was close to impossible to measure.

Social implications
Text.

Originality/value
This work is original because the Aachener Mentoring Modell entails 35 professional mentors working in all but one faculty of RWTH Aachen University; it is an entire schooling concept, with technical documentation especially implemented for this project.
Subjectivity in incentive schemes, cognitive orientations and counterproductive knowledge behavior: an experimental study
Authors: Mursita Lufi Yuwana , Almilia Luciana Spica 
Published: October 2020

Purpose
This study aims to examine the causal relationship of subjective incentive schemes on counterproductive knowledge behavior. Besides, this study also identifies the moderating role of cognitive orientation on the relationship between those two variables.

Design/methodology/approach
This study used a 2 × 2 between-subjects laboratory experiment with accounting undergraduate students as the subjects.

Findings
Subjective-based incentive schemes reduce the tendency for counterproductive knowledge behavior. Also, the collectivist cognitive orientation negatively influences the behavior. However, cognitive orientation does not act as a moderator in the causal relationship of incentive schemes and counterproductive knowledge behavior.

Originality/value
To the best of the authors’ knowledge, this study is the first that investigates and finds the effect of inclusion of subjectivity in incentive schemes and the level of individual’s collectivism on the reluctance to share knowledge in the workplace. This study has also strived to reduce an overlapping between the concept of knowledge sharing and counterproductive knowledge behavior by applying the right basic concept during the experiment.
Do local circumstances affect attitudes towards local social policies? Exploring the role of economic, political and demographic conditions
Authors: Broekema Babs , Fenger Menno , van der Waal Jeroen 
Published: October 2020

PurposeThis article aims to explore whether and how economic, political and demographic municipal conditions shape citizens' attitudes regarding decentralised social policies.Design/methodology/approachThe authors analysed the 2018 wave of the Dutch Local Election Studies, which includes a novel survey item asking respondents whether they prefer local social policies to be primarily: (1) protection-based, (2) cohesion-building or (3) activation-based. The authors appended context indicators to that survey and performed multilevel logistic regression analyses (1,913 respondents nested in 336 municipalities).FindingsAt the individual level, these preferences are affected by gender, age, income, education and political inclination, as expected. However, preferences towards local social policies are not shaped by local economic, demographic or political conditions. The authors discuss the implications of these findings for future research.Originality/valueBy using unique data, including a newly developed survey item, this study is the first to explore whether and how municipal conditions shape preferences regarding local welfare. Understanding those preferences is increasingly important as many Western European countries have decentralised swathes of social policies from the national to the local level in recent decades.
I am you, you are me: game character congruence with the ideal self
Authors: Ko Dong Woo , Park Jihye 
Published: October 2020

PurposeThe purpose of this research is to examine the effects of congruence between the ideal self-image of a game player and the game character on identification and interaction with the game character, perceived game power and performance, character attachment and willingness to spend money on the game character.Design/methodology/approachA total of 347 online game players participated in an online survey posted via the nationwide crowdsourcing web service Mechanical Turk in the US. A structural equation modelling was conducted using a maximum-likelihood estimation procedure to test the relationships among the variables.FindingsThe results revealed a significant positive impact of congruence between a game character and the ideal self-image of a game player on identification and interaction with the game character, perceived game power, game performance, attachment to the game character and willingness to spend money on the game character.Originality/valueAlthough significant research has been conducted in the area of online gaming, limited attention has been given to the strategic game content that stimulates a player's intention to purchase game items. Due to the challenges in sales growth in the game industry caused by business model shifts from a subscription-based model to a free-to-play one, it is important for marketing practitioners to motivate game players to continue playing the game and purchase game items. The results of this study provide valuable strategic insights to overcome the limitations of existing marketing strategies in the online game business.
Jaroslav Vanek, participation and labor management: an assessment including some bibliometric analysis
Authors: Jones Derek C. 
Published: October 2020

PurposeThe main aim of this paper is to provide an assessment of the intellectual impact of the work of Jaroslav Vanek in the related fields of participation and labor management (hereafter, PLM) and participation and employee ownership (hereafter, PEO).Design/methodology/approachThis paper used mixed methods including bibliometric analysis.FindingsVanek's work, particularly the General Theory of Labor-Managed Market Economies, (Vanek, 1970) is the building block for the modern scientific study of cooperatives and for helping to establish the fields of PLM and PEO. Vanek (1970) continues to be the highest cited publication each year that investigates the pure case of a labor-managed firm. Arguably his work has played a significant role is setting the stage for the development of adjacent fields in economics such as the new institutional economics. For an economist, his work has had an unusually strong impact on work outside of economics.Originality/valueNo similar assessment has been undertaken before.
Adaptive and noncyclic preventive maintenance to augment production activities
Authors: Dutta Sunil , Reddy Narala Suresh Kumar 
Published: October 2020

PurposeProduction schedules, if not met as per timelines may result in heavy losses to a company in terms of its standing and the overall profit. Production scheduling is generally planned by not taking preventive maintenance schedules into consideration. Most of the plants allocate discrete hours/time for preventive maintenance activities. These hours allocated for preventive maintenance will be in addition to the hours which would be lost during breakdown maintenance. These lost hours may be reduced if production scheduling and preventive maintenance activities are integrated. This advocates that we need to devise a methodology which can take care of lost hours.Design/methodology/approachAdaptive and noncyclic maintenance strategy describes the modification of existing maintenance practices, policies and procedures to meet new dynamic tasks/opportunities. It demands a high degree of flexibility and mental agility from maintenance staff members. The maintenance team has to be on a lookout for an opportunity message received from the central server and has to act promptly. The moment an opportunity arises, a message is forwarded to a central maintenance server (opportunity is captured). The central server then assigns individuals/team, based on their expertise and the maintenance task due on that machine/equipment. This action is completely automated and implemented without delay.FindingsThe total man-hours saved by executing adaptive and noncyclic preventive maintenance methodology comes to 705 h during 15 days on 30 machines installed in three different sections. There was a contribution of 71 innovative ideas from the repair teams. Out of these 71 innovative ideas, 16 were found suitable for execution. A quantum jump in the morale and motivation of the maintenance team was noticed from the feedback forms. Mutual understanding and respect for each other among employees has been enhanced. The optimization of resources and infrastructure including tools, gauges, testing equipment, etc. could truly be attained.Practical implicationsThe developed adaptive and noncyclic preventive maintenance model assists in capturing lost hours and make the system proactive and lean. The suggested model optimizes the preventive and predictive maintenance activities and results in substantial saving of efforts, manpower, resources and allocated budget.Originality/valueThe adaptive and noncyclic preventive maintenance model discussed in the article is a novel approach for the optimization of resources. The technique assists in capturing lost hours and utilization of these hours for preventive maintenance tasks. The model will also encourage creative and innovative ideas from employees and take the organization toward Continual Maintenance Optimization.