bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asymptomatic Carotid Atherosclerosis Cardiovascular Risk Factors and Common Hypertriglyceridemia Genetic Variants in Patients with Systemic Erythematosus Lupus
Authors: Fanlo-Maresma Marta , Candás-Estébanez Beatriz , Esteve-Luque Virginia , Padró-Miquel Ariadna , Escrihuela-Vidal Francesc , Carratini-Moraes Monica , Corbella-Ingles Emili , Corbella-Virós Xavier , Pintó-Sala Xavier 
Published: August 2020

Abstract
Background and aims: SLE is a systemic autoimmune disease associated with an increased cardiovascular risk which is related with the characteristic dyslipidemia of SLE. This consists of an alteration of triglyceride-rich lipoprotein metabolism and an increased concentration of apoB containing particles. The objective of this study is to know the prevalence of carotid atherosclerosis and to analyze its relationship with dyslipidemia and its related genetic factors in a population of SLE patients.Methods: Seventy-one SLE female were recruited. A carotid ultrasound was performed to evaluate the presence of atheromatous plaques and cIMT. Lipid profile and analysis of ZPR1, APOA5 and GCKR genes were carried out. SLE patients were analyzed according to the presence or absence of carotid plaques. Statistical analyses were performed to evaluate the relationship between lipid parameters and these allelic variants involved in triglyceride metabolism.Results: SLE patients with carotid plaque had higher concentrations of plasma triglyceride than SLE patients without carotid plaque (1.5 vs 0.9 mmol/L, respectively, p=0.001), Non-HDLC (3.5 vs 3.1 mmol/L, p= 0.025) and apoB (1.0 vs 0.9 g/L, p=0.010). GCKR (c.1337C>T) C-allele was observed in 83.3% and 16.7% (p=0.047) of patients with and without carotid plaque, respectively. The GCKR (c.1337C>T) CC genotype (OR= 0.03; [95% CI] [0.002 to 0.53], p=0.016), and triglyceride concentrations (OR= 7.57; [95% CI] [1.43 to 40.19], p=0.017) were independently associated with the diagnosis of carotid plaque.Conclusions: plasma triglyceride concentration and CGKR CC homozygosity for CGKR gene are independent predictive factors of carotid atherosclerosis in women with systemic lupus erythematosus.Keywords: triglycerides, CGKR gene, Non-HDLC, atherosclerosis, systemic lupus erythematosus.
Investigation Risk Factors for Breast Cancer in Women: A Case–Control Study in Arak, Iran
Authors: Pezeshki Milad , Ansari Jamshid , Rezaie Jafar , Ahmadloo Mojtaba 
Published: August 2020

Abstract
BackgroundBreast cancer is the most common malignant in women globally. In Iran, breast cancer incidence rate is continuously increasing. This study aimed to investigate the risk factors for breast cancer in Iranian women.MethodsA hospital-based case-control study was conducted between September 2016 and July 2019 in Arak, Iran. The sample size was 400 breast cancer patients and 400 healthy women. Demographical records and risk factor related data were collected. Logistic regression analysis used to calculate odds ratios (ORs) and 95% confidence intervals (CIs).ResultsData showed that among various factors, urban life (OR = 1.361, 95% CI 1.025–1.808, P = 0.033), height (OR = 3.347, 95% CI 2.0443–5.480, P = 0.000), BMI (OR = 0.397, 95% CI 0.273–0.577, P = 0.000), education level (OR = 7.048, 95% CI 3.985–12.467, P = 0.000), awareness level (OR = 0.507, 95% CI 0.349–0.736, P = 0.000), job status (OR = 0.321, 95% CI 0.122–0.846, P = 0.022), economic status (OR = 4.333, 95% CI 1.424–13.184, P = 0.010), early menarche (OR = 2.815, 95% CI 1.745–4.541, P = 0.000), Stillbirth status (OR = 1.935, 95% CI 1.087–3.446, P = 0.025), family history (OR = 10.281, 95% CI 3.628–29.134, P = 0.000), behavioral habits (OR = 0.554, 95% CI 0.386–0.796, P = 0.001), and second-hand smoking (OR = 1.472, 95% CI 1.108–1.955, P = 0.008) significantly were associated with an increased risk for breast cancer.ConclusionThe data suggest that lifestyle may have more impact on the incidence of breast cancer in Iranian women, suggesting change unhealthy lifestyle and screening for preventing breast cancer.
Exosomes Derived from Anoxia Preconditioned Mesenchymal Stem Cells alleviate Myocardial Ischemia Reperfusion Injury by Inhibiting Pyroptosis/ Caspase-1 Induced Apoptosis in The Deficiency of Gasdermin D
Authors: Mao Chengyu , Li Dongjiu , Zhou En , Gao Erhe , Zhang Tiantian , Gao Lin , Chen Kan , Wang Yue , Wang Changqian 
Published: August 2020

Abstract
BackgroundExosomes derived from adipose-derived mesenchymal stem cells can potentially protect cardiomyocytes from myocardial ischemia reperfusion injury. It's notable that exosomes derived from adipose-derived mesenchymal stem cells underwent anoxia preconditioning showed a better cardioprotective effect than that without anoxia. Here, in vitro and in vivo studies were used to investigate the cardioprotective effects against myocardial ischemia reperfusion injury of exosomes derived from adipose-derived mesenchymal stem cells with (Int-EXO) or without anoxia (NC-EXO), respectively. Methods: Adipose-derived mesenchymal stem cells and exosomes were identified by western blot, flow cytometry, transmission electron microscopy, and nanosight. An exosome tracer assay identified exosomes absorbed by cells. An in vitro model using mice cardiomyocytes for studying anoxia-reoxygenation and an in vivo mice model of MIRI were used to investigate the cardioprotective effects of NC-Exo and Int-Exo, respectively.ResultsWe discovered that treatment with NC-EXO and Int-EXO significantly reduced the infarct size and attenuated cardiomyocyte apoptosis, In addition, Int-EXO group had a less infarct size and apoptosis degree. The mechanism revealed by RNA sequencing showed that 40 miRNAs were up-regulated in Int-EXO compared to NC-EXO. 10 of these miRNAs could bind thioredoxin-interacting protein as a downstream target gene; among these, the top-discrepant miRNA224-5p was selected for further study. Dual luciferase reporter assay and rescue study verified TXNIP as a target gene for miR-224-5p. Furthermore, the cellular death signaling pathway which Int-EXO involved in mediating was in a special form of apoptosis, not pyroptosis, induced by activated thioredoxin-interacting protein-pyroptosis-caspase1 pathway in gasdermin D-deficient cells. ConclusionThe research demonstrated adipose-derived mesenchymal stem cells exosomes attenuated MIRI by inhibiting pyroptosis-induced apoptosis in cardiomyocytes which are lack of gasdermin D. The cardioprotective effect of Int-EXO was more significant than that of NC-EXO, possibly due to treated with anoxia preconditioning, adipose-derived mesenchymal stem cells product more miRNAs targeting thioredoxin-interacting protein in exosomes to alleviate pyroptosis-induced apoptosis. These findings provide new insights into the pathogenesis and methods for intervention of myocardial ischemia reperfusion injury.
Diabetic Patients with Comorbidities had Worse Outcomes When Suffered with COVID-19 and Acarbose might have Protective Effects
Authors: Hu Weihua , Luo Shunkui , Lu Zhanjin , Li Chang , Chen Qijian , Fan Yameng , Chen Zaishu , Wu Longlong , Ye Jianfang , Chen Shiyan , Tong Junlu , Wang Lingling , Mei Jin , Lu Hongyun 
Published: August 2020

Abstract
Background: Previous studies showed that diabetes was a common comorbidity of COVID-19, but the effects of diabetes or anti-diabetic drugs on the mortality of COVID-19 have not been well described. To investigate the outcome of different status (with or without comorbidity) and anti-diabetic medication before admission of diabetic patients after SARS-CoV-2 infected, we collected clinical data of COVID-19 patients from Hubei Province and compared between diabetes and non-diabetes.Methods: In this multicenter and retrospective study, we enrolled 1,422 cases of consecutive hospitalized patients from January 21, 2020 to March 25, 2020 at six hospitals in Hubei Province, China. The primary endpoint was in-hospital mortality.Results: Diabetes patients were 10-years older than non-diabetes (p<0.001), had higher prevalence of comorbidities such as hypertension (p<0.001), coronary heart disease (p<0.001), cerebrovascular disease (CVD) (p<0.001), chronic kidney disease (CKD) (p=0.007). The incidence of mortality (p=0.003) were more prevalent among the diabetes group. Further analysis revealed that diabetes patients who took alpha-glucosidase inhibitor (AGI) had lower mortality rate(p<0.01). Multivariable Cox regression showed that male sex, hypertension, CKD, CVD, age were risk factors for the mortality of COVID-19. Survival curve revealed that, compared with diabetes only group, the mortality was increased in diabetes with comorbidities (p=0.009), but had no significant difference in the non-comorbidity group, p=0.59).Conclusions: Patients with diabetes had worse outcome when suffered with COVID-19, however, it was not associated with diabetes itself but the comorbidities. Furthermore, the administration of AGI could reduce the risk of death in patients with diabetes.
Physicians’ Views on the Usefulness and Feasibility of Identifying and Disclosing Patients’ Last Phase of Life and their Experiences with Using the Surprise Question: A Focus Group Study
Authors: Owusuaa Catherine , Beelen Irene van , Heide Agnes van der , Rijt Carin C.D. van der 
Published: August 2020

Abstract
Background: Accurate assessment that a patient is in the last phase of life is a prerequisite for timely initiation of palliative care in patients with a life-limiting disease, such as advanced cancer or advanced organ failure. Several palliative care quality standards recommend the surprise question to identify those patients.Methods: Physicians’ views of identifying and disclosing the last phase of life and their experiences with using the surprise question for patients with advanced cancer or chronic obstructive pulmonary disease (COPD) were explored in a qualitative focus group study. Data were analyzed using thematic analysis.Results: Fifteen medical specialists and general practitioners participated in two focus groups. Themes discussed in the focus groups were: prediction and disclosure of the imminence of death. Participants thought prediction of imminent death, within one year, was important. The surprise question was considered a useful prognostic tool; its use is facilitated by its simplicity but hampered by its subjective character. The medical specialist was considered mainly responsible for prognosticating and gradual disclosing a patient’s imminent death. Physicians’ reluctance to disclose the imminence of death to a patient was related to the uncertainty around prognostication, concerns about depriving patients of hope or affecting the physician–patient relationship, or about a lack of time or of palliative care services. Conclusions: Physicians consider the assessment of patients’ imminent death important and support the use of the surprise question. However, they experience uncertainty and other barriers in disclosing imminent death. Future studies should examine patients’ preferences for those discussions. Trial registration: not applicable.
Bibliometric analysis of Scopus indexed publications on polymyxin resistance (2009-2018)
Authors: Quincho-Lopez Alvaro , Pacheco-Mendoza Josmel 
Published: August 2020

Abstract
Background: Antimicrobial resistance is a serious public health problem that has become a global threat. Special attention should be given to polymyxins (polymyxin B and colistin) which, since their reintroduction into clinical practice, are considered "last resort" drugs. The objective of this study is to perform a bibliometric analysis of scientific research on polymyxin resistance.Methods: Scopus database was used to retrieve documents relevant to polymyxin resistance. At the beginning, all kinds of documents without year restrictions were included. Subsequently, the study period (2009-2018) was limited and analyzed using SciVal. Specifically, the 10 institutions and countries with the highest production, authors, journals, and most cited articles were analyzed. The growth of publications and citations was graphed, in addition to an analysis of the keywords using VOSviewer.Results: A total of 1,306 documents were retrieved (1947-2019). Original articles (76.95%) and letters to the editor (15.47%) were the most frequent types of documents. English was the predominant language with 1,270 documents (97.2%). In the period 2009-2018, there was a significant growth in publications (p-value < 0.001) retrieving 833 (64%) documents. The received citations were 23,974, with a peak in 2016 (8,033 citations). The United States and China lead the scientific production with 146 (17.5%) and 137 (16.4%) publications, respectively. University of Fribourg (Switzerland) was the most productive institution on the subject (44 documents), although University of Zhejiang (China) has caused the greatest impact (73.5 citations/article). Antimicrobial Agents and Chemotherapy ranked first with 140 documents. Most of the documents were published in quartile 1 journals (82.7% and 69.1%, according to Scimago Journal & Country Rank and CiteScore, respectively).Conclusions: The number of documents on polymyxin resistance has increased significantly in the recent years. In the last few years alone, 64% of all documents have been published. The United States and China lead the scientific production. Greater efforts are still needed to tackle this global problem.
Long Noncoding RNA NORAD/MiR-26a-5p/NAMPT (Visfatin) Axis Regulates Proliferation and Apoptosis of Human Osteosarcoma Cells
Authors: Cheng Gong , Huang Botao , Cao Zhilin , Zhang Tongqing , Zhao Yuchi , Wu Hao 
Published: August 2020

Abstract
Background: Visfatin is a novel adipokine, also known as a nicotinamide phosphorybosyltransferase (NAMPT) that is reported to promote the progression of osteosarcoma. The research sought to determine the regulatory network underlying NAMPT on osteosarcoma (OS).Method: The OS tissues and paired normal controls were collected from 45 OS patients. The binding relationship between microRNA-26a-5p (miR-26a-5p) and two genes (NAMPT and NORAD) were predicted by TargetScan V7.2 and confirmed by dual-luciferase reporter assay. To reveal the function of long noncoding RNA NORAD/miR-26a-5p/NAMPT axis on cell viability, cell cycle and apoptosis of U2OS cells, CCK-8 and flow cytometry assays, examination of apoptosis-associated molecules (Bcl-2, Bax, cleaved (C)-caspase-3) were performed. The mRNA and protein levels were separately examined by RT-qPCR and Western blot. Results: NAMPT and NORAD expressions were increased in OS tissue samples, while miR-26a-5p expression was decreased. Functionally, NORAD functions as a ceRNA of miR-136-5p to competitively target NAMPT. Furthermore, miR-26a-5p overexpression inhibited viability, cell cycle and apoptosis resistance of U2OS cells by down-regulating NAMPT along with change the expressions of apoptosis-related molecules. NORAD overexpression promoted viability, cell cycle and apoptosis resistance of U2OS cells by down-regulating MiR-26a-5p along with changes of the expressions of apoptosis-related molecules.Conclusion: LncRNA NORAD, serving as a ceRNA of miR-26a-5p, promoted proliferation and apoptosis resistance of U2OS cells by upregulation of NAMPT.
High Expression of Growth Factor Receptor-bound Protein 14 Predicts Clinical Progression and Poor Prognosis in Patients with Lung Adenocarcinoma
Authors: Chen Qixian , Liu Zhaohui , Zhong Lintao , Liang Zhike , Song Haoyu , Li Jun , Yang Jian , Chen Lijun , Weng Yuqing 
Published: August 2020

Abstract
Background: Growth factor receptor-bound protein 14 (Grb14) is an adaptor molecule of the Grb7/10/14 family with a characteristic “between PH and SH2” (BPS) domain that serves to tightly bind tyrosine kinases. Previous studies have demonstrated that Grb14 upregulation may be used as a marker of proliferation, invasion and malignant cell growth in tumors. The overexpression of Grb14 has also been reported to be associated with a poor prognosis in cancer patients. However, the clinical significance of Grb14 in lung adenocarcinoma has not yet been fully elucidated.Methods: Grb14 protein expression in human lung adenocarcinoma and noncancerous lung tissues was detected by immunohistochemistry analyses. Then, the associations of Grb14 expression with clinicopathological features and clinical outcomes of lung cancer patients were validated by analyzing a microarray-based TCGA dataset at the mRNA level and statistically evaluating the results.Results: Immunohistochemistry and dataset analyses revealed that Grb14 expression was significantly increased in lung adenocarcinoma tissues compared with noncancerous lung tissues [immunoreactivity score (IRS): lung adenocarcinoma, 6.07±1.01 vs benign, 4.80±1.22, P<0.001]. Additionally, as revealed by analysis of the dataset, the upregulation of Grb14 mRNA expression in the lung adenocarcinoma tissues was significantly correlated with poor overall survival (P<0.001). Furthermore, univariate analysis revealed that higher pathological stage [hazard ratio (HR), 1.925, 95% CI, 0.912-6.301; P<0.05], higher tumor stage (HR, 2.436, 95% CI, 1.659-5.551; P<0.001), higher surgical margin resection status (HR, 3.035, 95% CI, 1.305-37.51; P<0.01) and prior diagnosis status (HR, 0.4893, 95% CI, 0.1818-0.8197; P<0.01) were independent predictors for a shorter survival. Multivariate analysis also revealed that higher pathological stage (HR, 3.730, 95% CI, 1.784-7.796; P<0.01), higher surgical margin resection status (HR, 6.914, 95% CI, 2.063-23.171; P<0.01) and prior diagnosis status were related to a shorter survival. Conclusion: Our data suggest that elevated Grb14 expression plays an important role in the progression of lung adenocarcinoma. More importantly, the increased expression of Grb14 may efficiently predict poor survival in lung adenocarcinoma patients.
Comparative analysis of whole chloroplast genomes of Ligusticum sinense and L. jeholense, Umbelliferae
Authors: Wang Shijie , Zhang Tingting , Xu Liang , Zhan Zhilai , Bao Guihua , Zhang Tong , Ding Zixuan , Sun Nan , Sun Shaobin , Xie Ming , Kang Tingguo 
Published: August 2020

Abstract
Background

Gaoben has a long history of application as medicine. There are few records of Liaogaoben in ancient books. The varieties of Ligusticum in practical application are confused. Therefore, it is very important to identify Ligusticum accurately. The phylogenetic position of Ligusticum in Umbelliferae needs to be determined. It is also of great significance to analyze the phylogeny of Ligusticum in Umbelliferae the difference of somatic genome.
Methods

Chloroplast (cp) genomic DNA was extracted from two species of Ligusticum and sequenced on Hiseq4000 light source. The sequence was assembled into contrags by soapenovo 2.04, aligned with reference genome by blast, and then corrected manually. Genereanation is performed by online dogma tools. The general characteristics of cp genomes of two species were analyzed, and compared with the relative species. The DNA of chloroplasts of higher plants is double stranded covalently closed loop molecule, and its length varies with species. According to the assembly genome sequence of the sequenced samples, combined with the prediction results of the coding genes, the genome of the samples was displayed in circles. After alignment, the evolutionary tree was constructed based on the single nucleotide polymorphism (SNP) of cp genome in 31 species by ML method.
Results

The whole chloroplast genome of Gaoben and Liaogaoben is 148,515 bp and 148,493 bp, both of which contain IR (IRa and IRb) LSC and SSC. 127 genes have been annotated, including 83 protein coding genes, 8 tRNA and 36 rRNA of all species, and 28 coding genes in IR region. There are six genes in the ATPase subunit of photosynthesis gene group, and there are obvious differences in the types of introns in NADH dehydrogenase subunit between them. In the comparative analysis of Pi value, two significant gene variation points are petG gene and psaL-ycf4 gene. The phylogenetic tree of the whole cp genome of SNP Umbelliferae was constructed, including 28 Umbelliferae and 3 Ligusticum.
Conclusions

In this study, the cp genomic characteristics of Ligusticum sinense and L. jeholense were identified, which provided a theoretical basis and documentation for the identification and phylogenetic analysis of Ligusticum.
Helicobacter pylori-induced Rev-erbα fosters gastric bacterial colonization by impairing host innate and adaptive defense
Authors: Zhuang Yuan , Mao Fangyuan , Lv Yipin , Hao Chuanjie , Teng Yongsheng , Liu Yugang , Cheng Ping , Yang Shiming , Chen Weisan , Liu Tao , Zou Quanming 
Published: August 2020

Abstract
Background: Helicobacter pylori (H. pylori) is a human pathogen that infects nearly half of the world’s population, however, the persistent colonization of H. pylori in gastric mucosa remains poorly understood. Nowadays it is believed that impairment of host defense of gastric epithelium induced by H. pylori plays key roles in H. pylori-associated pathology. The nuclear receptor Rev-erbα represents a powerful transcriptional repressor involved in host immunity. However, the regulation, function, and clinical relevance of Rev-erbα in H. pylori infection are presently unknown. Here we demonstrated a pro-colonization role of Rev-erbα in H. pylori infection.Results: Rev-erbα was increased in gastric mucosa of H. pylori-infected patients and mice. H. pylori induced gastric epithelial cells (GECs) to express Rev-erbα via the phosphorylated cagA that activated extracellular signal-regulated kinase (ERK) signaling pathway to mediate transcription factor nuclear factor kappa-B (NF-κB) directly binding to Rev-erbα promoter. Human gastric Rev-erbα expression correlated with H. pylori colonization, and mouse Rev-erbα from non-bone marrow-derived cells promoted gastric H. pylori burden. Importantly, H. pylori colonization was attenuated in Rev-erbα-/- mice and the mice with in vivo pharmacological inhibition of Rev-erbα. Mechanistically, Rev-erbα in GECs not only directly suppressed Reg3b and β-defensin-1 expression via binding to Reg3b and β-defensin-1 promoter respectively, which resulted in impaired bactericidal effects against H. pylori of these antibacterial proteins in vitro and in vivo; but also directly inhibited chemokine CCL21 expression via binding to CCL21 promoter, which led to decreased gastric influx of CD45+CD11c-Ly6G-CD11b+CD68- myeloid cells by CCL21-CCR7-dependent migration and, as a direct consequence, reduced bacterial clearing capacity of H. pylori-specific T helper type 1 (Th1) cell response. Conclusions: Overall, this study identifies a model involving Rev-erbα, which collectively ensures gastric bacterial persistence by suppressing host gene expression required for local innate and adaptive defense against H. pylori, and also highlight a pathological role and an immunosuppressive mechanism of Rev-erbα in persistent H. pylori infection.