bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustainability indices for energy utilization using a multi-criteria decision model
Authors: Suganthi L. 
Published: March 2020

Abstract
Background
Energy and water availability are considered vital for a country’s development. An exhaustive review of literature has been undertaken on indicators for sustainable energy development. In addition to energy and water, the resources of the country namely land availability, human capital, and nation’s wealth were also found to be vital for sustainable energy development of any country. These resources have to be optimally used for sustainable energy development which is measured in terms of technical, economic, social, environmental, and institutional indices.

Methods
In this research, a multi-criteria decision model was used to measure sustainable energy development efficiency. The natural resource of the country was considered as the input criteria, while the indices were used as the output criteria. This research is unique in finding if the country’s resources are being utilized in an efficient manner for sustainable development using the multi-criteria fuzzy-analytical hierarchical processing—data envelopment analysis model.

Results
From the fuzzy analytical hierarchical processing, it was found that experts were of the opinion that water availability and energy reserves were the most important parameters among the input resources, while environmental and social indices were opined to be the most important parameters among the output indices. Data envelopment analysis model was carried out using assurance region Charnes, Cooper, and Rhodes output-oriented method. The results revealed that Togo was the most efficient country with maximum sustainable energy development efficiency. Sensitivity analysis was also carried out to find which among the resources were sensitive with respect to energy sustainability for a specific country.

Conclusion
The analysis can help countries to benchmark themselves against the country lying in the efficient frontier and draft policies to improve their energy sustainability indices. Similar analysis and comparison can be done for other countries, since this research analysis was carried out only for 48 developing countries.
miR-34a targets PAI-1 to regulate urinary microalbumin and renal function in hypertensive mice
Authors: Liu Ruitao , Yang Lihong , Wei Qingmin 
Published: March 2020

Abstract
Background
The aim of the study is to investigate the effects of miR-34a targeted at PAI-1 on urinary microalbumin and renal function in hypertensive mice.

Methods
Twenty specific-pathogen-free (SPF) BPN/3J mice were selected in normal group, and 120 SPF BPH/2J mice were evenly divided into model group, negative control group, miR-34a mimic group, miR-34a inhibitor group, Si-PAI-1 group, and miR-34a inhibitor + Si-PAI-1 group. qRT-PCR was used to detect the expression of miR-34a and PAI-1 mRNA. The protein expressions of PAI-1, angiotensin-converting enzyme (ACE) and ACE2 were detected by Western blot. Serum levels of AngII and Ang1-7 were detected by ELISA.

Results
miR-34a negatively regulated the expression of PAI-1. Compared with the normal group, mice in the other groups had significantly lower body weight, increased systolic blood pressure and 24-h urinary microalbumin content, decreased miR-34a expression, superoxide dismutase (SOD) and nitric oxide (NO) content, and ACE2 protein expression, and increased PAI-1 expression, serum creatinine (Scr), blood urea nitrogen (BUN) malondialdehyde (MDA), AngII and Ang1-7 levels, and ACE protein expression (all P < 0.05). Compared with the model group, mice in the miR-34a mimic group and Si-PAI-1 group had no significant changes in body weight (all P > 0.05), while they had significantly lower systolic blood pressure and 24-h urinary microalbumin content, increased SOD and NO levels and ACE2 protein expression, and decreased PAI-1 expression, Scr, BUN, MDA, AngII and Ang1-7 levels, and ACE protein expression (all P < 0.05). Compared with the miR-34a inhibitor group, symptoms in miR-34a inhibitor + Si-PAI-1 group were significantly improved (all P < 0.05).

Conclusions
miR-34a can inhibit the expression of PAI-1, thereby reducing urinary microalbumin content in hypertensive mice and protecting their renal function.
Constitutive Model for Aggregate Interlock in FEM Analyses of Concrete Interfaces with Embedded Steel Bars
Authors: Figueira Diogo , Sousa Carlos , Neves Afonso Serra 
Published: March 2020

AbstractA nonlinear finite element model (FEM) is developed to assess the behaviour of a cracked concrete interface, reinforced with embedded steel bars and subjected to monotonic loading. A dowel action finite element modelling approach is conceived for that purpose. The bond between the steel bars and the surrounding concrete is also considered in the model and an interface finite element is included to simulate aggregate interlock. Then, the comparison of the model results with experimental values allowed the calibration of aggregate interlock constitutive relations for cracks in monolithic concrete restrained by embedded steel bars. New constitutive relations are also proposed for shear transfer by aggregate interlock in a concrete joint.
A case of ruptured mucinous cystadenoma of the pancreas with recurrence-free survival for 8 years
Authors: Fujinaga Atsuro , Hirashita Teijiro , Iwashita Yukio , Nakanuma Hiroaki , Tada Kazuhiro , Masuda Takashi , Endo Yuichi , Ohta Masayuki , Ono Hideki , Murakami Kazunari , Inomata Masafumi 
Published: March 2020

Abstract
Background
Pancreatic mucinous cystic neoplasm (MCM) presenting with rupture is extremely rare, and very few studies have followed up patients over the long term after ruptured mucinous cystadenoma (MCA). We report a case of ruptured MCA of the pancreas with recurrence-free survival for 8 years.

Case presentation
A 28-year-old Japanese woman was admitted to the emergency department of a local hospital after experiencing acute abdominal pain. Abdominal computed tomography revealed massive ascites and the presence of a cystic tumor measuring 60 mm in diameter in the pancreatic tail. Conservative therapy with antibiotics and abdominal drainage were performed to treat peritonitis that occurred secondary to the ruptured pancreatic cystic tumor, after which the patient’s symptoms improved. The patient was referred to our department for further examination and treatment. We diagnosed a ruptured MCN and performed laparoscopic spleen-preserving distal pancreatectomy. Histopathological findings revealed ovarian-type stroma, which tested positive for estrogen and progesterone receptors by immunohistochemistry. The histopathological diagnosis was MCA. The postoperative course was uneventful, and the patient remains alive without any evidence of recurrence at 8 years postoperatively.

Conclusion
A good prognosis is possible even in cases of ruptured MCA. Because of the risk of peritoneal dissemination after ruptured MCA, long-term follow-up is important.
Role of three-dimensional transvaginal sonography compared with magnetic resonance imaging in diagnosis of Mullerian duct anomalies
Authors: Abd elsalam Sahar Mahmoud , Abd elmegeed Naglaa Ezzat , Said Ahmed Hesham Mohammed , Sayed Mohamed Abd elghafar 
Published: March 2020

Abstract
Background
Mullerian duct anomalies are a heterogenous group of congenital anomalies due to abnormalities during the ductal development, fusion, or alteration of septal resorption. These lead to the occurrence of different uterine anomalies. The present study aimed to assess the value of three-dimensional transvaginal sonography (3DTVS) in comparison with pelvic MRI to diagnose uterine anomalies. We prospectively evaluated 30 female patients, from January 2016 to May 2017. Their ages ranged from 18 to 40 years. Cases were referred from obstetrics and gynecology clinic with suspected uterine anomalies by 2DUS or with HSG done for infertility workup. 3DTVS and MRI examination were done for all patients.

Results
The final diagnosis of patients by MRI according to The European Society of Human Reproduction and Embryology–European Society for Gynaecological Endoscopy consensus classification included 2/30 (6.7%) patients classified as class U0, 4/30 (13.3%) patients classified as class U1, 17/30 (56.7%) cases classified as class U2, 2/30 (6.7%) patients classified as class U3, 3/30 (10%) patients classified as class U4, and 2/30 (6.7%) patients were classified as class U5. There was a significant strong agreement between the diagnosis by 3D-transvaginal ultrasound and MRI (P < 0.01).

Conclusion
3DTVS proved to be highly accurate in the diagnosis of uterine anomalies and showed strong agreement with MRI, as both can provide valuable information about both the internal details and the external contour of the uterus.
Customer gender prediction system on hierarchical E-commerce data
Authors: Khan Mohammad Masud , Sohrab Mohammad Golam , Yousuf Mohammad Abu 
Published: March 2020

Abstract
Background
E-commerce services provide online shopping sites and mobile applications for small and medium sellers. To provide more efficient buying and selling experiences, a machine learning system can be applied to predict the optimal organization and display of products that maximize the chance of bringing useful information to user that facilitate the online purchases. Therefore, it is important to understand the relevant products for a gender to facilitate the online purchases. In this work, we present a statistical machine learning (ML)-based gender prediction system to predict the gender “male” or “female” from transactional E-commerce data. We introduce different sets of learning algorithms including unique IDs decomposition, context window-based history generation, and extract identical hierarchy from training set to address the gender prediction classification system from online transnational data.

Results
The experiment result shows that different feature augmentation approaches as well as different term or feature weighting approaches can significantly enhance the performance of statistical machine learning-based gender prediction system.

Conclusions
This work presents a ML-based implementational approach to address E-commerce-based gender prediction system. Different session augmentation approaches with support vector machines (SVMs) classifier can significantly improve the performance of gender prediction system.
Magnetic target recognition and localization method unaffected by attitude
Authors: Zhi Huiqiang , Ma Tiehua , Pei Dongxin , Sun Hexuan 
Published: March 2020

Abstract
This letter presents a magnetic target inversion method that does not vary with changes in the coordinate system and is based on the cross-product of the intermediate eigenvectors of any two points in the dipole field which is in the same/opposite direction as the magnetic moment vector. We used tensor geometric invariants to interpret this new physical property and obtain the unit magnetic moment vector. Using this, the unit vector of the measurement point-source displacement vector was derived. The distance between the measurement point and the source was obtained via the Frobenius norm of the gradient tensor matrix. Simulations verified that the proposed method is unaffected by attitudes and yields unique inversion results, and the results revealed that the inversion accuracy of the proposed method is high. The simulation results also show that conditional cosine and measurement noise have considerable influence on the inversion accuracy in the proposed method.
Insulin Autoimmune Syndrome: A Case of Clopidogrel-induced Autoimmune Hypoglycemia
Authors: Calder Genevieve L , Ward Glenn M , Sachithanandan Nirupa , MacIsaac Richard J 
Published: March 2020

Abstract

Context
Insulin autoimmune syndrome (IAS) is characterized by hyperinsulinemic hypoglycemia with elevated anti-insulin antibodies. Most commonly observed in the Japanese population, elsewhere it is rare and associated with autoimmune diseases, plasma cell dyscrasias, or sulfhydryl group medications. The active metabolite of clopidogrel has a sulfhydryl group and here we report a case of clopidogrel-induced IAS.

Case Description
A 67-year-old man was admitted with severe hyperinsulinemic hypoglycemia requiring continuous intravenous infusion of 10% dextrose to sustain euglycemia. His symptoms of hypoglycemia had started after commencing dual antiplatelet therapy (including clopidogrel) for ischemic heart disease 9 months earlier. The hypoglycemia was associated with elevated insulin, proinsulin, c-peptide, and anti-insulin antibody titers as well as the HLA-DRB1*04 haplotype. Multiple localizing studies were negative for an insulinoma. A diagnosis of IAS was thus made. Clopidogrel cessation, oral dexamethasone, and diazoxide therapy were not sufficient to safely wean the dextrose infusion. Plasma exchange was ultimately effective.

Conclusions
This case highlights a case of severe IAS. Given the ubiquity of clopidogrel, IAS should be remembered as a rare adverse effect.
Dynorphin and GABAA Receptor Signaling Contribute to Progesterone’s Inhibition of the LH Surge in Female Mice
Authors: Liu Yali , Li Xiaofeng , Shen Xi , Ivanova Deyana , Lass Geffen , He Wen , Chen Qiuju , Yu Sha , Wang Yun , Long Hui , Wang Li , Lyu Qifeng , Kuang Yanping , O’Byrne Kevin T 
Published: March 2020

Abstract
Progesterone can block estrogen-induced luteinising hormone (LH) surge secretion and can be used clinically to prevent premature LH surges. The blocking effect of progesterone on the LH surge is mediated through its receptor in the anteroventral periventricular nucleus (AVPV) of the hypothalamus. However, the underlying mechanisms are unclear. The preovulatory LH surge induced by estrogen is preceded by a significant reduction in hypothalamic dynorphin and gamma-aminobutyric acid (GABA) release. To test the detailed roles of dynorphin and GABA in an LH surge blockade by progesterone, ovariectomized and 17β-estradiol capsule-implanted (OVX/E2) mice received simultaneous injections of estradiol benzoate (EB) and progesterone (P) or vehicle for 2 consecutive days. The LH level was monitored from 2:30 pm to 8:30 pm at 30-minute intervals. Progesterone coadministration resulted in the LH surge blockade. A continuous microinfusion of the dynorphin receptor antagonist nor-BNI or GABAA receptor antagonist bicuculline into the AVPV from 3:00 pm to 7:00 pm reversed the progesterone-mediated blockade of the LH surge in 7 of 9 and 6 of 10 mice, respectively. In addition, these LH surges started much earlier than the surge induced by estrogen alone. However, 5 of 7 progesterone-treated mice did not show LH surge secretion after microinfusion with the GABAB receptor antagonist CGP-35348. Additionally, peripheral administration of kisspeptin-54 promotes LH surge-like release in progesterone treated mice. These results demonstrated that the progesterone-mediated suppression of the LH surge is mediated by an increase in dynorphin and GABAA receptor signaling acting though kisspeptin neurons in the AVPV of the hypothalamus in female mice.
Is Mitochondrial Dysfunction a Common Root of Noncommunicable Chronic Diseases?
Authors: Diaz-Vegas Alexis , Sanchez-Aguilera Pablo , Krycer James R , Morales Pablo E , Monsalves-Alvarez Matías , Cifuentes Mariana , Rothermel Beverly A , Lavandero Sergio 
Published: March 2020

AbstractMitochondrial damage is implicated as a major contributing factor for a number of noncommunicable chronic diseases such as cardiovascular diseases, cancer, obesity, and insulin resistance/type 2 diabetes. Here, we discuss the role of mitochondria in maintaining cellular and whole-organism homeostasis, the mechanisms that promote mitochondrial dysfunction, and the role of this phenomenon in noncommunicable chronic diseases. We also review the state of the art regarding the preclinical evidence associated with the regulation of mitochondrial function and the development of current mitochondria-targeted therapeutics to treat noncommunicable chronic diseases. Finally, we give an integrated vision of how mitochondrial damage is implicated in these metabolic diseases.
STRIPAK–PP2A regulates Hippo-Yorkie signaling to suppress retinal fate in the Drosophila eye disc peripodial epithelium
Authors: Neal Scott J. , Zhou Qingxiang , Pignoni Francesca 
Published: March 2020

ABSTRACT
The specification of organs, tissues and cell types results from cell fate restrictions enacted by nuclear transcription factors under the control of conserved signaling pathways. The progenitor epithelium of the Drosophila compound eye, the eye imaginal disc, is a premier model for the study of such processes. Early in development, apposing cells of the eye disc are established as either retinal progenitors or support cells of the peripodial epithelium (PE), in a process whose genetic and mechanistic determinants are poorly understood. We have identified protein phosphatase 2A (PP2A), and specifically a STRIPAK–PP2A complex that includes the scaffolding and substrate-specificity components Cka, Strip and SLMAP, as a critical player in the retina-PE fate choice. We show that these factors suppress ectopic retina formation in the presumptive PE and do so via the Hippo signaling axis. STRIPAK–PP2A negatively regulates Hippo kinase, and consequently its substrate Warts, to release the transcriptional co-activator Yorkie into the nucleus. Thus, a modular higher-order PP2A complex refines the activity of this general phosphatase to act in a precise specification of cell fate.
SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network
Authors: Chakraborty Archan , Lin Wei-Cheng , Lin Yu-Tsun , Huang Kuang-Jing , Wang Pei-Yu , Chang Ian Yi-Feng , Wang Hsiang-Iu , Ma Kung-Ting , Wang Chun-Yen , Huang Xuan-Rong , Lee Yen-Hsien , Chen Bi-Chang , Hsieh Ya-Ju , Chien Kun-Yi , Lin Tzu-Yang , Liu Ji-Long , Sung Li-Ying , Yu Jau-Song , Chang Yu-Sun , Pai Li-Mei 
Published: March 2020

ABSTRACT
Under metabolic stress, cellular components can assemble into distinct membraneless organelles for adaptation. One such example is cytidine 5′-triphosphate synthase (CTPS, for which there are CTPS1 and CTPS2 forms in mammals), which forms filamentous structures under glutamine deprivation. We have previously demonstrated that histidine (His)-mediated methylation regulates the formation of CTPS filaments to suppress enzymatic activity and preserve the CTPS protein under glutamine deprivation, which promotes cancer cell growth after stress alleviation. However, it remains unclear where and how these enigmatic structures are assembled. Using CTPS–APEX2-mediated in vivo proximity labeling, we found that synaptosome-associated protein 29 (SNAP29) regulates the spatiotemporal filament assembly of CTPS along the cytokeratin network in a keratin 8 (KRT8)-dependent manner. Knockdown of SNAP29 interfered with assembly and relaxed the filament-induced suppression of CTPS enzymatic activity. Furthermore, APEX2 proximity labeling of keratin 18 (KRT18) revealed a spatiotemporal association of SNAP29 with cytokeratin in response to stress. Super-resolution imaging suggests that during CTPS filament formation, SNAP29 interacts with CTPS along the cytokeratin network. This study links the cytokeratin network to the regulation of metabolism by compartmentalization of metabolic enzymes during nutrient deprivation.
Characterization of unconventional kinetochore kinases KKT10 and KKT19 in Trypanosoma brucei
Authors: Ishii Midori , Akiyoshi Bungo 
Published: March 2020

ABSTRACT
The kinetochore is a macromolecular protein complex that drives chromosome segregation in eukaryotes. Unlike most eukaryotes that have canonical kinetochore proteins, evolutionarily divergent kinetoplastids, such as Trypanosoma brucei, have unconventional kinetochore proteins. T. brucei also lacks a canonical spindle checkpoint system, and it therefore remains unknown how mitotic progression is regulated in this organism. Here, we characterized, in the procyclic form of T. brucei, two paralogous kinetochore proteins with a CLK-like kinase domain, KKT10 and KKT19, which localize at kinetochores in metaphase but disappear at the onset of anaphase. We found that these proteins are functionally redundant. Double knockdown of KKT10 and KKT19 led to a significant delay in the metaphase to anaphase transition. We also found that phosphorylation of two kinetochore proteins, KKT4 and KKT7, depended on KKT10 and KKT19 in vivo. Finally, we showed that the N-terminal part of KKT7 directly interacts with KKT10 and that kinetochore localization of KKT10 depends not only on KKT7 but also on the KKT8 complex. Our results reveal that kinetochore localization of KKT10 and KKT19 is tightly controlled to regulate the metaphase to anaphase transition in T. brucei.
This article has an associated First Person interview with the first author of the paper.
The ubiquitin hydrolase Doa4 directly binds Snf7 to inhibit recruitment of ESCRT-III remodeling factors in S. cerevisiae
Authors: Buysse Dalton , Pfitzner Anna-Katharina , West Matt , Roux Aurélien , Odorizzi Greg 
Published: March 2020

ABSTRACT
The ESCRT-III protein complex executes reverse-topology membrane scission. The scission mechanism is unclear but is linked to remodeling of ESCRT-III complexes at the membrane surface. At endosomes, ESCRT-III mediates the budding of intralumenal vesicles (ILVs). In Saccharomyces cerevisiae, ESCRT-III activity at endosomes is regulated through an unknown mechanism by Doa4, an ubiquitin hydrolase that deubiquitylates transmembrane proteins sorted into ILVs. We report that the non-catalytic N-terminus of Doa4 binds Snf7, the predominant ESCRT-III subunit. Through this interaction, Doa4 overexpression alters Snf7 assembly status and inhibits ILV membrane scission. In vitro, the Doa4 N-terminus inhibits association of Snf7 with Vps2, which functions with Vps24 to arrest Snf7 polymerization and remodel Snf7 polymer structure. In vivo, Doa4 overexpression inhibits Snf7 interaction with Vps2 and also with the ATPase Vps4, which is recruited by Vps2 and Vps24 to remodel ESCRT-III complexes by catalyzing subunit turnover. Our data suggest a mechanism by which the deubiquitylation machinery regulates ILV biogenesis by interfering with ESCRT-III remodeling.
EML4–ALK V3 oncogenic fusion proteins promote microtubule stabilization and accelerated migration through NEK9 and NEK7
Authors: O'Regan Laura , Barone Giancarlo , Adib Rozita , Woo Chang Gok , Jeong Hui Jeong , Richardson Emily L. , Richards Mark W. , Muller Patricia A. J. , Collis Spencer J. , Fennell Dean A. , Choi Jene , Bayliss Richard , Fry Andrew M. 
Published: March 2020

ABSTRACT
EML4–ALK is an oncogenic fusion present in ∼5% of non-small cell lung cancers. However, alternative breakpoints in the EML4 gene lead to distinct variants of EML4–ALK with different patient outcomes. Here, we show that, in cell models, EML4–ALK variant 3 (V3), which is linked to accelerated metastatic spread, causes microtubule stabilization, formation of extended cytoplasmic protrusions and increased cell migration. EML4–ALK V3 also recruits the NEK9 and NEK7 kinases to microtubules via the N-terminal EML4 microtubule-binding region. Overexpression of wild-type EML4, as well as constitutive activation of NEK9, also perturbs cell morphology and accelerates migration in a microtubule-dependent manner that requires the downstream kinase NEK7 but does not require ALK activity. Strikingly, elevated NEK9 expression is associated with reduced progression-free survival in EML4–ALK patients. Hence, we propose that EML4–ALK V3 promotes microtubule stabilization through NEK9 and NEK7, leading to increased cell migration. This represents a novel actionable pathway that could drive metastatic disease progression in EML4–ALK lung cancer.