bluuebunny/crossref_metadata_2025_split huggingface dataset:
protein-sol pKa: prediction of electrostatic frustration, with application to coronaviruses
Authors: Hebditch Max , Warwicker Jim 
Published: April 2020

AbstractEvolution couples differences in ambient pH to biological function through protonatable groups, in particular those that switch from buried to exposed and alter protonation state in doing so. We present a tool focusing on structure-based discovery and display of these groups. Since prediction of buried group pKas is computationally intensive, solvent accessibility of ionisable groups is displayed, from which the user can iteratively select pKa calculation centers. Results are color-coded, with emphasis on buried groups. Utility is demonstrated with coronaviruses, which exhibit variable dependence on the acidic pH of the endocytotic pathway. After benchmarking with variants of murine hepatitis virus, a pair of conserved histidine residues are identified that are predicted to be electrostatically frustrated at acidic pH in a common structural core of pre- and post-fusion coronavirus spike proteins. We suggest that an intermediate expanded conformation at endosomal pH could relax the frustration, allowing histidine protonation, and facilitating conformational conversion. This tool is available athttp://www.protein-sol.manchester.ac.uk/pka/.
Localized mRNA translation mediates maturation of cytoplasmic cilia in Drosophila spermatogenesis
Authors: Fingerhut Jaclyn M , Yamashita Yukiko M 
Published: April 2020

AbstractCytoplasmic cilia, a specialized type of cilia in which the axoneme resides within the cytoplasm rather than within the ciliary compartment, are proposed to allow the efficient assembly of very long cilia. Despite being found diversely in male gametes (e.g. Plasmodium microgametocytes and human and Drosophila sperm), very little is known about cytoplasmic cilia assembly. Here we show that a novel RNP granule containing the mRNAs for axonemal dynein motor proteins becomes highly polarized to the distal end of the cilia during cytoplasmic ciliogenesis in Drosophila sperm. This allows for the localized translation of these axonemal dyneins and their incorporation into the axoneme directly from the cytoplasm. We found that this RNP granule contains the proteins Reptin and Pontin, loss of which perturbs granule formation and prevents incorporation of the axonemal dyneins, leading to sterility. We propose that cytoplasmic cilia require the local translation of key protein constituents such that these proteins are incorporated efficiently into the axoneme.Author SummaryCytoplasmic cilia, which are found in human and Drosophila sperm, are unique in that the axoneme is exposed to the cytoplasm. The authors show that a novel RNP granule containing axonemal dynein mRNAs facilitates localized translation of these axonemal proteins, facilitating cytoplasmic cilia formation.
GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells
Authors: Vitillo Loriana , Durance Catherine , Hewitt Zoe , Moore Harry , Smith Austin , Vallier Ludovic 
Published: April 2020

ABSTRACTA major challenge for the clinical use of human pluripotent stem cells is the development of safe, robust and controlled differentiation protocols. Adaptation of research protocols using reagents designated as research-only to those which are suitable for clinical use, often referred to as Good Manufacturing Practice (GMP) reagents, is a crucial and laborious step in the translational pipeline. However, published protocols to assist this process remain very limited. Here we present a new GMP-compliant protocol to derive long-term neuroepithelial stem cell progenitors (lt-NES), which are multipotent, bankable, and karyotypically stable. This protocol resulted in robust and reproducible differentiation of several clinical-grade embryonic stem cells, deposited in the UK Stem Cell Bank, from which we derived lt-NES. Furthermore, GMP-derived lt-NES demonstrated a high neurogenic potential while retaining the ability to be redirected to several neuronal sub-types. Overall, we report the feasibility of derivation and differentiation of clinical grade embryonic stem cell lines into lt-NES under GMP-compliant conditions. Our protocols could be used as a flexible tool to speed up translation-to-clinic of pluripotent stem cells for a variety of neurological therapies or regenerative medicine studies.
TBX3 acts as tissue-specific component of the Wnt/β-catenin enhanceosome
Authors: Zimmerli Dario , Borrelli Costanza , Jauregi-Miguel Amaia , Söderholm Simon , Brütsch Salome , Doumpas Nikolaos , Reichmuth Jan , Murphy-Seiler Fabienne , Aguet Michel , Basler Konrad , Moor Andreas E. , Cantù Claudio 
Published: April 2020

AbstractBCL9 and PYGO are β-catenin cofactors that enhance the transcription of Wnt target genes. They have been proposed as therapeutic targets to diminish Wnt signalling output in intestinal malignancies. Here we find that, in colorectal cancer cells and in developing mouse forelimbs, BCL9 proteins sustain the action of β-catenin in a largely PYGO-independent manner. Our genetic analyses implied that BCL9 necessitates other interaction partners in mediating its transcriptional output. We identified the transcription factor TBX3 as a candidate tissue-specific member of the β-catenin transcriptional complex. In developing forelimbs, TBX3 and BCL9 co-occupy a large number of Wnt-responsive regulatory elements, genome-wide. Moreover, mutations inBcl9affect the expression of TBX3 targets in vivo, and modulation of TBX3 abundance impacts on Wnt target genes transcription in a β-catenin- and TCF/LEF-dependent manner. Finally, TBX3 overexpression exacerbates the metastatic potential of Wnt-dependent human colorectal cancer cells. Our work implicates TBX3 as a new, context-dependent component of the Wnt/β-catenin-dependent enhanceosome.
The number of growing microtubules and nucleus-nucleus interactions uniquely regulate nuclear movement inDrosophilamuscle
Authors: Collins Mary Ann , Coon L. Alexis , Thomas Riya , Mandigo Torrey R. , Wynn Elizabeth , Folker Eric S. 
Published: April 2020

ABSTRACTNuclear movement is a fundamental process of eukaryotic cell biology. Skeletal muscle presents an intriguing model to study nuclear movement because its development requires the precise positioning of multiple nuclei within a single cytoplasm. Furthermore, there is a high correlation between aberrant nuclear positioning and poor muscle function. Although many genes that regulate nuclear movement have been identified, the mechanisms by which these genes act is not known. UsingDrosophila melanogastermuscle development as a model system, and a combination of live-embryo microscopy and laser ablation of nuclei, we have found that phenotypically similar mutants are based in different molecular disruptions. Specifically, ensconsin (DrosophilaMAP7) regulates the number of growing microtubules that are used to move nuclei whereas bocksbeutel (Drosophilaemerin) and klarsicht (DrosophilaKASH-protein regulate interactions between nuclei.
Radiochromic film dosimetry in synchrotron radiation breast computed tomography: a phantom study
Authors: Mettivier Giovanni , Masi Marica , Arfelli Fulvia , Brombal Luca , Delogu Pasquale , Di Lillo Francesca , Donato Sandro , Fedon Christian , Golosio Bruno , Oliva Piernicola , Rigon Luigi , Sarno Antonio , Taibi Angelo , Russo Paolo 
Published: April 2020

This study relates to the INFN project SYRMA-3D for in vivo phase-contrast breast computed tomography using the SYRMEP synchrotron radiation beamline at the ELETTRA facility in Trieste, Italy. This peculiar imaging technique uses a novel dosimetric approach with respect to the standard clinical procedure. In this study, optimization of the acquisition procedure was evaluated in terms of dose delivered to the breast. An offline dose monitoring method was also investigated using radiochromic film dosimetry. Various irradiation geometries have been investigated for scanning the prone patient's pendant breast, simulated by a 14 cm-diameter polymethylmethacrylate cylindrical phantom containing pieces of calibrated radiochromic film type XR-QA2. Films were inserted mid-plane in the phantom, as well as wrapped around its external surface, and irradiated at 38 keV, with an air kerma value that would produce an estimated mean glandular dose of 5 mGy for a 14 cm-diameter 50% glandular breast. Axial scans were performed over a full rotation or over 180°. The results point out that a scheme adopting a stepped rotation irradiation represents the best geometry to optimize the dose distribution to the breast. The feasibility of using a piece of calibrated radiochromic film wrapped around a suitable holder around the breast to monitor the scan dose offline is demonstrated.
Investigation and multi-scale optimization design of woven composite cut-out structures
Authors: Nguyen Duc Hai , Wang Hu , Ye Fan , Hu Wei 
Published: April 2020

Purpose
The purpose of this paper is to investigate the mechanical properties’ behaviors of woven composite cut-out structures with specific parameters. Because of the complexity of micro-scale and meso-scale structure, it is difficult to accurately predict the mechanical material behavior of woven composites. Numerical simulations are increasingly necessary for the design and optimization of test procedures for composite structures made by the woven composite. The results of the proposed method are well satisfied with the results obtained from the experiment and other studies. Moreover, parametric studies on different plates based on the stacking sequences are investigated.

Design/methodology/approach
A multi-scale modeling approach is suggested. Back-propagation neural networks (BPNN), radial basis function (RBF) and least square support vector regression are integrated with efficient global optimization (EGO) to reduce the weight of assigned structure. Optimization results are verified by finite element analysis.

Findings
Compared with other similar studies, the advantage of the suggested strategy uses homogenized properties behaviors with more complex analysis of woven composite structures. According to investigation results, it can be found that 450/−450 ply-orientation is the best buckling load value for all the cut-out shape requirements. According to the optimal results, the BPNN-EGO is the best candidate for the EGO to optimize the woven composite structures.

Originality/value
A multi-scale approach is used to investigate the mechanical properties of a complex woven composite material architecture. Buckling of different cut-out shapes with the same area is surveyed. According to investigation, 45°/−45° ply-orientation is the best for all cut-out shapes. Different surrogate models are integrated in EGO for optimization. The BPNN surrogate model is the best choice for EGO to optimization difficult problems of woven composite materials.
Does political instability influence dividend payout policy: evidence from Tunisian Stock Exchange?
Authors: Loukil Nadia 
Published: April 2020

PurposeThe purpose of this study tests whether political instability influence financial decision-making behavior of Tunisian-listed firms, in particular dividend payout policy.Design/methodology/approachThis paper uses dividend payout decisions announced over the period 2008–2015 by nonfinancial firms listed on the Tunisian Stock Exchange. A logistic regression is applied to analyze the relationship between political instability and dividend payout decision “changes. These latter are: past non-payers” dividend initiation, past payers' dividend termination, dividend payout “increasing and dividend payout” decreasing. Political instability variables used are as follows: number of changes in government head and dummy variables indicating the changes of ruling party and election year.FindingsThis study shows that government head changes are positively related to dividend initiation decisions while changes in ruling party are negatively related to termination dividend decisions except for family controlled ones. These firms are more likely to stop dividend on period of ruling party changes. Moreover, firms become unwilling to increase dividend payment on the period of political instability (changes in ruling party and government head and elections) and become willing to decrease dividend payment only when the government head changes.Practical implicationsThe empirical findings contribute to the current debate on the signaling power of dividend policy in emerging market where raising equity capital is difficult and controlling shareholders prefer reinvest benefit to pay dividends. In addition, this study has important implications for regulators and governments struggling to design policies to improve investors' confidence and boost market activity. Indeed, investors may use corporate payout as a signal for better governance.Originality/valueTo the author' best knowledge, this paper is the first to investigate and to compare the effect of three political instability sources; government head changes, changes in ruling party and elections, on dividend payout decision changes. This paper provides evidence that firms facing political unstable environment seek to achieve two goals when they make dividend policy: reducing financial distress probability and attracting minority owners.
HRM practices and innovation: an empirical systematic review
Authors: Easa Nasser Fathi , Orra Haitham El 
Published: April 2020

Purpose
The relationship between human resource management practices (HRMP) and innovation has been described as a black box, where a lot still needs to be investigated. Thus, the aim of this paper is to investigate the nature of the link that exists between HRMP and innovation in both public and private organizations. To do so, theoretical underpinnings and existence of a mediating or a moderating mechanism is inspected.

Design/methodology/approach
Based on an empirical systematic review of research conducted between 2010 and 2018, content analysis has been conducted for 31 peer-reviewed articles in the English language.

Findings
Inspecting the nature of relations existed in the chosen articles, interesting findings are addressed relative to the nature of the human resource management systems (HRMS) used, practices encompassed and their different utility. HRMS has been shown to be associated with product innovation yet more evidence is needed for supporting process innovation.

Practical implications
The HRMS/HRMP and innovation relationship is inspected, important practices that would guide managers to induce innovation are highlighted. Usage of multiple HRMS and contingency in constructing such systems is indicated.

Originality/value
Contribution to comprehend the black box and areas for future research has been offered.
Discomfort and organizational change as a part of becoming a world-class university
Authors: Nadia Fiona Niska Dinda , Sukoco Badri Munir , Susanto Ely , Sridadi Ahmad Rizki , Nasution Reza Ashari 
Published: April 2020

PurposeThis study examined organizational change in universities as it relates to discomfort among the organization's members.Design/methodology/approachUsing the critical incident technique (CIT), data was collected from the informants in an Indonesian public university that had been mandated by the government to enter the top 500 world university ranking. This would make it a “World-Class” university.FindingsThe findings describe the causes, courses and consequences of the discomfort felt in response to the organizational change in the university context. The causes of discomfort were categorized as a fear of loss, organizational culture, systems and policies, work overload and a lack of resources. Discomfort can manifest through negative affective, cognition and behavioral tendencies. Meanwhile, the consequences result in active and passive participation in the process of the organizational change itself.Originality/valueDiscomfort with organizational change is a new variable that has rarely been explored, thus it requires testing and validation using different methods and contexts, as offered by this study. We have also shown that in the initial stage of organizational change (unfreezing), discomfort will always emerge that must be immediately managed in order not to trigger resistance to change. Furthermore, this study exhibits the use of the critical incident technique in the context of organizational change. Finally, we offer comprehensive views by exhibiting the causes, the reactions shown and the consequences of discomfort with the change.
Exploring the relationship between school leadership and teacher professional learning through structural equation modeling
Authors: Tayag Jarrent , Ayuyao Nunilon 
Published: April 2020

PurposeThis study aims to investigate the relationship between leadership and teacher professional learning considering two mediating variables – teacher trust and teacher agency.Design/methodology/approachThe study utilized structural equation modeling in analyzing the responses from 1,654 secondary public teachers from 43 schools in the Philippines.FindingsLeadership does not have a direct relationship with teacher professional learning. The established relationship of leadership and professional learning from literature exists through the mediation of teacher trust and teacher agency.Originality/valueThe results point out that school leaders must affect teacher trust and teacher agency to influence the engagement of teachers to professional learning. Contrary to what has been accepted in educational management that leaders can directly impact teachers, the findings of this study dictate that the full effects of leadership are coursed through mediating pathways.
Exploring employee performance dimensionality in Indian public sector units
Authors: Pattnaik Subhra , Pattnaik Susmita 
Published: April 2020

PurposePerformance is considered to be a multi-dimensional construct with three underlying dimensions such as Task Performance (TP), Interpersonal Facilitation (IPF), and Job Dedication (JD). Yet, these dimensions exhibit high inter-correlations in many studies. This study explores the dimensionality of performance as perceived by managers in Indian Public Sector Units (PSUs) and its implications on the sustainability of performance practices in these organizations.Design/methodology/approachPerformance data of 588 PSU executives was obtained from their respective managers (162 in number) using a pen-paper survey. Exploratory Factor Analysis (EFA) followed by a Confirmatory Factor Analysis (CFA) was employed to test the dimensionality of the performance construct using SPSS 25 and AMOS 24.FindingsThe data obtained had best fit when performance was modelled as a second order factor with the three dimensions drawing on it as first order indicators. However, the measurement model with employee performance modelled as a one-dimensional first order factor had poor fit. This indicates that PSU managers do perceive an implicit differentiation between the performance dimensions, but the performance ratings do not explicitly reflect that differentiation.Originality/valueTo the best of the authors' knowledge, this is the first ever study in Indian context that explores the perception of PSU managers on performance dimensionality. It discusses if Indian PSUs could sustain the dynamism of the future workplace with the present performance management practices and makes relevant suggestions in that direction.
Therapy with exonerated clients: review and recommendations
Authors: Heilbrun Kirk , Fishel Sarah , Lankford Claire , Ratkalkar Mina 
Published: April 2020

Purpose
The conviction of innocent individuals has emerged as an international concern, resulting in substantial attention to the legal needs that stem from exoneration. However, many other challenges can also arise in the aftermath of an exoneration, including financial, psychosocial and mental health needs. Relatively little has been written about the particular reentry needs of individuals who are exonerated of their charges, and even fewer studies have considered the effectiveness of various treatment approaches. The purpose of this paper is to reviews the available literature, identifies gaps and provides clinical recommendations for the development of treatment interventions for exonerees.

Design/methodology/approach
The research addressing the needs and challenges that arise in the aftermath of exoneration is reviewed and analysed for implications that can guide treatment-planning in this area.

Findings
This paper reviews key finds from the literature and provides recommendations for developing a semi-structured approach to treating exonerees.

Practical implications
Practical applications for the development of effective therapeutic interventions for exonerated individuals are identified and discussed.

Originality/value
Currently, there is very limited literature addressing the specific reentry needs and effective therapeutic interventions for exonerated individuals.
Complainers' resource investment and mobilization in digital environments using Conservation of Resources theory
Authors: Beh Yean Shan , Sajtos Laszlo , Cao Joanne T. 
Published: April 2020

PurposeThe purpose of this paper is to consider whether consumers can recover from a service failure by utilizing internal and external energy resources that are available to them at the time of an online complaint. Based on the Conservation of Resources (COR) theory, this research conceptualizes the complainers' act of complaining through internal and external energy resources. By investing (direct utilization of resources) and mobilizing (utilizing resources to change the trajectory of a loss) these resources, this study aims to understand which resources (internal or external) and what strategies (investment or mobilization) are effective in the face of a resource loss.Design//methodology/approachStudy 1 aimed to test the impact of energy resources (motivation and affordance) on consumers' negative emotions and satisfaction with their complaints through an online panel survey. Study 2 was a between-subjects design experiment aimed to overcome the diversity of the circumstances around a service failure, complaint motivation and complaints that were captured in Study 1.FindingsThis study provides evidence of the negative and positive effects of internal and external energy resources, respectively, in altering the consumer's emotions and behavioral intentions. The findings of this study underline the role of affordances of features, specifically perceived conversationality of digital features, in improving consumers' relationship with the defaulting firm.Practical implicationsBased on the findings related to the perceived conversationality of digital features, managers are urged to explore the affordances of online features that consumers use for communications, in general, or for complaints, in particular.Originality/valueTo our understanding, this paper is the first study to employ COR theory as a conceptual background, and in turn, the first to conceptualize complaint motivations and online complaint features as internal and external resources, respectively. As such, this study is the first of its kind to examine complaint media systematically.