bluuebunny/crossref_metadata_2025_split huggingface dataset:
A highly specific and sensitive serological assay detects SARS-CoV-2 antibody levels in COVID-19 patients that correlate with neutralization
Authors: Peterhoff David , Glück Vivian , Vogel Matthias , Schuster Philipp , Schütz Anja , Neubert Philip , Albert Veruschka , Frisch Stefanie , Kiessling Mara , Pervan Philip , Werner Maren , Ritter Nicole , Babl Leon , Deichner Maria , Hanses Frank , Lubnow Matthias , Müller Thomas , Lunz Dirk , Hitzenbichler Florian , Audebert Franz , Hähnel Viola , Offner Robert , Müller Martina , Schmid Stephan , Burkhardt Ralph , Glück Thomas , Koller Michael , Niller Hans Helmut , Graf Bernhard , Salzberger Bernd , Wenzel Jürgen J. , Jantsch Jonathan , Gessner André , Schmidt Barbara , Wagner Ralf 
Published: August 2020

AbstractObjectiveThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic challenges national health systems and the global economy. Monitoring of infection rates and seroprevalence can guide public health measures to combat the pandemic. This depends on reliable tests on active and former infections. Here, we set out to develop and validate a specific and sensitive enzyme linked immunosorbent assay (ELISA) for detection of anti-SARS-CoV-2 antibody levels.MethodsIn our ELISA, we used SARS-CoV-2 receptor-binding domain (RBD) and a stabilized version of the spike (S) ectodomain as antigens. We assessed sera from patients infected with seasonal coronaviruses, SARS-CoV-2 and controls. We determined and monitored IgM-, IgA- and IgG-antibody responses towards these antigens. In addition, for a panel of 22 sera, virus neutralization and ELISA parameters were measured and correlated.ResultsThe RBD-based ELISA detected SARS-CoV-2-directed antibodies, did not cross-react with seasonal coronavirus antibodies and correlated with virus neutralization (R2 = 0.89). Seroconversion started at 5 days after symptom onset and led to robust antibody levels at 10 days after symptom onset. We demonstrate high specificity (99.3%;N = 1000) and sensitivity (92% for IgA, 96% for IgG and 98% for IgM; > 10 days after PCR-proven infection;N = 53) in serum.ConclusionsWith the described RBD-based ELISA protocol, we provide a reliable test for seroepidemiological surveys. Due to high specificity and strong correlation with virus neutralization, the RBD ELISA holds great potential to become a preferred tool to assess thresholds of protective immunity after infection and vaccination.
Copeptin – Stellenwert in der Diagnostik des Polyurie-Polydipsie-Syndroms
Authors: Christ-Crain Mirjam 
Published: August 2020

ZusammenfassungDas Polyurie-Polydipsie-Syndrom beinhaltet drei verschiedene Entitäten, den zentralen Diabetes insipidus, den nephrogenen Diabetes insipidus und die primäre Polydipsie. Eine zuverlässige Unterscheidung ist essenziell, da die jeweilige Therapie unterschiedlich ist und eine unkorrekte Behandlung zu potenziell gefährlichen Komplikationen führen kann. Jahrzehntelang war der klassische Durstversuch der Goldstandard, hatte jedoch diverse Limitationen und eine eingeschränkte diagnostische Zuverlässigkeit.Copeptin ist ein stabiler und zuverlässiger Surrogatmarker von Vasopressin und spiegelt die Vasopressinkonzentration in der Zirkulation wider. Mit der Verfügbarkeit von Copeptinassays wurde die Differenzialdiagnose des Polyurie-Polydipsie-Syndroms neu evaluiert. Ein basaler Copeptinwert ohne vorheriges Dursten kann einen nephrogenen Diabetes insipidus diagnostizieren. Für die Unterscheidung eines zentralen Diabetes insipidus von einer primären Polydipsie braucht es eine osmotische (mittels hypertoner Kochsalzlösung) oder nichtosmotische (mittels Arginin) Stimulation. Beide Tests können mit höherer diagnostischer Zuverlässigkeit als der Durstversuch diese beiden Entitäten unterscheiden. Entsprechend sollte ein Copeptin-basierter Algorithmus als der neue Goldstandard für die Differenzialdiagnose des Polyurie-Polydipsie-Syndroms eingeführt werden.
Teachers’ Self-efficacy in Preventing and Intervening in School Bullying: a Systematic Review
Authors: Fischer Saskia M. , John Nancy , Bilz Ludwig 
Published: August 2020

AbstractSelf-efficacy, commonly seen as an important competence for teachers when intervening in bullying, is a heterogeneous construct. Differences in the specific kinds of self-efficacy under review, its assessment, theoretical foundations, and the samples used when researching it generate diverse results that can be hard to integrate. This systematic review surveys existing literature on the extent of teachers’ self-efficacy in managing bullying and its connection to the likelihood that teachers will intervene in bullying, to their intervention strategies, and the prevention measures they employ, as well as students’ bullying behavior and their experiences of victimization. Thirty-six relevant publications based on 33 studies were included. The quality of their results was assessed, and their findings were systematized and are discussed here. Many studies are of low quality because of incomplete descriptions of underlying theoretical as well as methodological foundations. Almost all results are based on descriptive study designs. Consistent findings show that teachers generally feel confident in managing bullying and that teachers who are more confident intervene more often. Findings were mixed on the connections between teachers’ self-efficacy and intervention strategies, and the prevention measures they used, as well as students’ bullying behavior and experiences of victimization. It was also discovered that the connections between teachers’ self-efficacy and their prevention measures, their students’ bullying behavior, and collective efficacy in general have rarely been addressed in the literature.
Updated Cariaco Basin14C Calibration Dataset from 0–60 cal kyr BP
Authors: Hughen Konrad A , Heaton Timothy J 
Published: August 2020

ABSTRACTWe present new updates to the calendar and radiocarbon (14C) chronologies for the Cariaco Basin, Venezuela. Calendar ages were generated by tuning abrupt climate shifts in Cariaco Basin sediments to those in speleothems from Hulu Cave. After the original Cariaco-Hulu calendar age model was published, Hulu Cave δ18O records have been augmented with increased temporal resolution and a greater number of U/Th dates. These updated Hulu Cave records provide increased accuracy as well as precision in the final Cariaco calendar age model. The depth scale for the Ocean Drilling Program Site 1002D sediment core, the primary source of samples for14C dating, has been corrected to account for missing sediment from a core break, eliminating age-depth anomalies that afflicted the earlier calendar age models. Individual14C dates for the Cariaco Basin remain unchanged from previous papers, although detailed comparisons of the Cariaco calibration dataset to those from Hulu Cave and Lake Suigetsu suggest that the Cariaco marine reservoir age may have shifted systematically during the past. We describe these recent changes to the Cariaco datasets and provide the data in a comprehensive format that will facilitate use by the community.
The Relevance of Pyrogenic Carbon for Carbon Budgets From Fires: Insights From the FIREX Experiment
Authors: Santin Cristina , Doerr Stefan H. , Jones Matthew W. , Merino Agustin , Warneke Carsten , Roberts James M. 
Published: August 2020

AbstractVegetation fires play an important role in global and regional carbon cycles. Due to climate warming and land use shifts, fire patterns are changing and fire impacts increasing in many of the world's regions. Reducing uncertainties in carbon budgeting calculations from fires is therefore fundamental to advance our current understanding and forecasting capabilities. Here we study 20 chamber burns from the FIREX FireLab experiment, which burnt a representative set of North American wildland fuels, to assess the following: (i) differences in carbon emission estimations between the commonly used “consumed biomass” approach and the “burnt carbon” approach; (ii) pyrogenic carbon (PyC) production rates; and (iii) thermal and chemical recalcitrance of the PyC produced, as proxies of its biogeochemical stability. We find that the “consumed biomass” approach leads to overestimation of carbon emissions by 2–27% (most values between 2% and 10%). This accounting error arises largely from not considering PyC production and, even if relatively small, can therefore have important implications for medium‐ and long‐term carbon budgeting. A large fraction (34–100%) of this PyC was contained in the charred fine residue, a postfire material frequently overlooked in fire carbon research. However, the most recalcitrant PyC was in the form of woody charcoal, with estimated half‐lives for most samples exceeding 1,000 years. Combustion efficiency was relatively high in these laboratory burns compared to actual wildland fire conditions, likely leading to lower PyC production rates. We therefore argue that the PyC production values obtained here, and associated overestimation of carbon emissions, should be taken as low‐end estimates for wildland fire conditions.
Tearing Instability in Alfvén and Kinetic‐Alfvén Turbulence
Authors: Boldyrev Stanislav , Loureiro Nuno F. 
Published: August 2020

AbstractRecently, it has been realized that magnetic plasma turbulence and magnetic field reconnection are inherently related phenomena. Turbulent fluctuations generate regions of sheared magnetic field that become unstable to the tearing instability and reconnection, thus modifying turbulence at the corresponding scales. In this contribution, we give a brief review of some recent results on tearing‐mediated magnetic turbulence. We illustrate the main ideas of this rapidly developing field of study by concentrating on two important examples—magnetohydrodynamic Alfvén turbulence and small‐scale kinetic‐Alfvén turbulence. Due to various potential applications of these phenomena in space physics and astrophysics, we specifically try not to overload the text by heavy analytical derivations but rather present a qualitative discussion accessible to a non‐expert in the theories of turbulence and reconnection.
Atomic‐Scale Simulations of Meteor Ablation
Authors: Guttormsen Gabrielle , Fletcher Alex C. , Oppenheim Meers M. 
Published: August 2020

AbstractMeteoroids smaller than a microgram constantly bombard the Earth, depositing material in the mesosphere and lower thermosphere. Meteoroid ablation, the explosive evaporation of meteoroids due to erosive impacts of atmospheric particles, consists of sputtering and thermal ablation. This paper presents the first atomic‐scale modeling of sputtering, the initial stage of ablation where hypersonic collisions between the meteoroid and atmospheric particles cause the direct ejection of atoms from the meteoroid surface. Because meteoroids gain thermal energy from these particle impacts, these interactions are important for thermal ablation as well. In this study, a molecular dynamics simulator calculates the energy distribution of the sputtered particles as a function of the species, velocity, and angle of the incoming atmospheric particles. The sputtering yield generally agrees with semi‐empirical equations at normal incidence but disagrees with the generally accepted angular dependence. Λ, the fraction of energy from a single atmospheric particle impact incorporated into the meteoroid, was found to be less than 1 and dependent on the velocity, angle, atmospheric species, and meteoroid material. Applying this new Λ to an ablation model results in a slower meteoroid temperature increase and mass loss rate as a function of altitude. This alteration results in changes in the expected electron line densities and visual magnitudes of meteoroids. Notably, this analysis leads to the prediction that meteoroids will generally ablate 1–4 km lower than previously predicted. This affects analysis of radar and visual measurements, as well as determination of meteoroid mass.
Origin and Distribution of Daytime Electron Density Irregularities in the Low‐Latitude F Region
Authors: Kil Hyosub , Lee Woo Kyoung , Paxton Larry J. 
Published: August 2020

AbstractElectron density irregularities on the dayside in the low‐latitude F region are understood as remnants (or fossils) of nighttime plasma bubbles. We provide observational evidence of the connection of daytime irregularities to nighttime bubbles and the transport of the daytime irregularities by the vertical motion of the background ionosphere. The distributions of irregularities are derived using the measurements of the ion density by the first Republic of China satellite from March 1999 to June 2004. The seasonal and longitudinal distributions of daytime and nighttime irregularities in low latitudes show a close similarity. The high occurrence rate of daytime irregularities at the longitudes where strong irregularities occur frequently at night provides strong evidence of the association of daytime irregularities with nighttime bubbles. Nighttime irregularities are concentrated in the equatorial region, whereas daytime irregularities spread over broader latitudes. The seasonal and longitudinal variation of the latitudinal spread of daytime irregularities is consistent with the morphologies of plasma density and vertical plasma velocity. The zonal wave number 4 pattern, which corresponds to that in plasma density, is identified in the distribution of daytime irregularities. These observations lead to the conclusion that the morphology of daytime irregularities in the low‐latitude F region is dominated by the morphology of bubbles at night and the ionospheric fountain process on the dayside.
Modeling the Evolution of Aquatic Organisms in Dynamic River Basins
Authors: Stokes Maya F. , Perron J. Taylor 
Published: August 2020

AbstractRivers are conduits for aquatic organisms and host an exceptional number of species. Over geologic time, rivers and the aquatic organisms that live in them are subject to changes in topography that can alter where rivers flow. Differences in erosion rates across drainage divides cause some river basins to grow and others to shrink. Occasionally, rivers are abruptly rerouted by river captures that create both new dispersal corridors and barriers for aquatic organisms. These changes in habitat connectivity can lead to the evolution of new species, which has prompted suggestions that river captures may be a mechanism to produce high freshwater biodiversity. We test this hypothesis by building a model, Bio‐SLANT (Biodiversity on Simulated LAndscapes using Neutral Theory). Bio‐SLANT couples a computational landscape model that simulates river basin reorganization to a macroevolutionary model that simulates the dispersal, speciation, and extinction of organisms. We first show that modeled basin area exerts a primary control on within‐basin species richness due to the species‐area relationship. We then describe the effects of drainage area exchange between river basins. River capture increases species richness, but only temporarily, whereas elevated rates of speciation and extinction provide a persistent biological record of river network reorganization. When river captures are frequent, speciation rates increase more than extinction rates, resulting in a positive diversification rate under most of the biological parameters tested. We explore the implications of our results for species richness in landscapes with basins of different relative sizes and for diversification in tectonically active and inactive settings.
Retreating Glacier and Advancing Forest Over the Past 200 Years in the Central Himalayas
Authors: Sigdel Shalik Ram , Zhang Hui , Zhu Haifeng , Muhammad Sher , Liang Eryuan 
Published: August 2020

AbstractThe Himalayan glaciers, as unique reservoirs of freshwater for densely populated Asian countries, have been receiving considerable attention in public and scientific research. However, little is known about their long‐term fluctuations as related to climate variability. Herein, we reconstructed glacier fluctuation events by tree ring‐based moraine dating and repeated photographs. We assessed these fluctuations over the past 200 years in the Gangapurna and Annapurna III glaciers, located in the central Himalayas. We detected five major glacier fluctuation events in the 1790s, 1920s, 1930s, 1960s, and 1970s. A higher rate of glacier recession was observed from the 1980s onward, coinciding with climate warming. As shown by repeated photographs, the retreating rate of the Gangapurna glacier from 1979 to 2016 (up to 24.4 m yr−1) is much higher than the Annapurna III glacier (7.54 m yr−1) from 1950 to 2016. Their different frontal retreat rates were likely related to microtopography and glacier sizes. Older moraines developed during and after the Little Ice Age (LIA) were already covered by dense forest, presenting the evidence for retreating glaciers and advancing forests in the central Himalayas. Ongoing warming tends to speed up such ecological processes by resetting biophysical environment of the deglaciated forefields. In general, glaciers have shown retreating trends over the last 200 years, presenting a risk for preserving water resources in the Himalayas and nearby regions. Alternatively, advancing forest tends to amplify warming rate by reducing albedo, which is a new issue for investigating ecohydrological feedbacks between mountain forests, climate, and water resources.
Loss of symbiont infectivity following thermal stress can be a factor limiting recovery from bleaching in cnidarians
Authors: Kishimoto Mariko , Baird Andrew H , Maruyama Shinichiro , Minagawa Jun , Takahashi Shunichi 
Published: August 2020

Abstract
Increases in seawater temperature can cause coral bleaching through loss of symbiotic algae (dinoflagellates of the family Symbiodiniaceae). Corals can recover from bleaching by recruiting algae into host cells from the residual symbiont population or from the external environment. However, the high coral mortality that often follows mass-bleaching events suggests that recovery is often limited in the wild. Here, we examine the effect of pre-exposure to heat stress on the capacity of symbiotic algae to infect cnidarian hosts using the Aiptasia (sea-anemone)-Symbiodiniaceae model system. We found that the symbiont strain Breviolum sp. CS-164 (ITS2 type B1), both free-living and in symbiosis, loses the capacity to infect the host following exposure to heat stress. This loss of infectivity is reversible, however, a longer exposure to heat stress increases the time taken for reversal. Under the same experimental conditions, the loss of infectivity was not observed in another strain Breviolum psygmophilum CCMP2459 (ITS2 type B2). Our results suggest that recovery from bleaching can be limited by the loss of symbiont infectivity following exposure to heat stress.
Long noncoding RNA UCA1 from hypoxia-conditioned hMSC-derived exosomes: a novel molecular target for cardioprotection through miR-873-5p/XIAP axis
Authors: Sun Ling , Zhu Wenwu , Zhao Pengcheng , Wang Qingjie , Fan Baohan , Zhu Yeqian , Lu Yao , Chen Qiushi , Zhang Jian , Zhang Fengxiang 
Published: August 2020

AbstractExosomes (Exo) secreted from mesenchymal stem cells (hMSCs) are protective against myocardial injury. The purpose of the study was to investigate the role and mechanisms by which exosomes promote cardiomyocyte survival and function following myocardial infarction (MI). hMSCs were cultured under hypoxic and normoxic conditions. Hypoxia-conditioned hMSC-derived exosomes (Hypo-Exo) and normoxic-conditioned hMSC-derived exosomes (Nor-Exo) were collected and intramyocardially injected into rats with MI. The therapeutic effects of Hypo-Exo and Nor-Exo were evaluated after 4 weeks. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of candidate long noncoding RNA urothelial carcinoma associated 1 (lncRNA-UCA1) in Nor-Exo and Hypo-Exo. Intramyocardial injection of lncRNA-UCA1-knockdown-Hypo-Exo in a rat model of MI was then performed and the cardiac function was characterized. The target and downstream of the molecular mechanism lncRNA-UCA1 was disclosed by luciferase reporter assays and western blot. Circulating exosomal lncRNA-UCA1 level in AMI patients and healthy volunteers was assessed. We found that (1) hMSC exosomal (from hypoxic and normoxic conditions) cardioprotection in vitro and in vivo correlated with the presence of encapsulated lncRNA-UCA1 in exosomes; (2) lncRNA-UCA1 targeted miR-873 via sponging, reducing the latter’s suppressive effects on its target XIAP, and this translated into AMPK phosphorylation and increased level of the antiapoptotic protein BCL2; and (3) plasma derived from patients with AMI contained exosomes enriched with the lncRNA-UCA1, unlike that from normal subjects. This study demonstrates that Hypo-Exo lncRNA-UCA1 plays a cardioprotective role via the miR-873-5p/XIAP axis and circulating exosomal lncRNA-UCA1 may be a promising novel biomarker for the diagnosis of AMI.
Combined treatment with CBP and BET inhibitors reverses inadvertent activation of detrimental super enhancer programs in DIPG cells
Authors: Wiese Maria , Hamdan Feda H. , Kubiak Klaudia , Diederichs Christopher , Gielen Gerrit H. , Nussbaumer Gunther , Carcaboso Angel M. , Hulleman Esther , Johnsen Steven A. , Kramm Christof M. 
Published: August 2020

AbstractDiffuse intrinsic pontine gliomas (DIPG) are the most aggressive brain tumors in children with 5-year survival rates of only 2%. About 85% of all DIPG are characterized by a lysine-to-methionine substitution in histone 3, which leads to global H3K27 hypomethylation accompanied by H3K27 hyperacetylation. Hyperacetylation in DIPG favors the action of the Bromodomain and Extra-Terminal (BET) protein BRD4, and leads to the reprogramming of the enhancer landscape contributing to the activation of DIPG super enhancer-driven oncogenes. The activity of the acetyltransferase CREB-binding protein (CBP) is enhanced by BRD4 and associated with acetylation of nucleosomes at super enhancers (SE). In addition, CBP contributes to transcriptional activation through its function as a scaffold and protein bridge. Monotherapy with either a CBP (ICG-001) or BET inhibitor (JQ1) led to the reduction of tumor-related characteristics. Interestingly, combined treatment induced strong cytotoxic effects in H3.3K27M-mutated DIPG cell lines. RNA sequencing and chromatin immunoprecipitation revealed that these effects were caused by the inactivation of DIPG SE-controlled tumor-related genes. However, single treatment with ICG-001 or JQ1, respectively, led to activation of a subgroup of detrimental super enhancers. Combinatorial treatment reversed the inadvertent activation of these super enhancers and rescued the effect of ICG-001 and JQ1 single treatment on enhancer-driven oncogenes in H3K27M-mutated DIPG, but not in H3 wild-type pedHGG cells. In conclusion, combinatorial treatment with CBP and BET inhibitors is highly efficient in H3K27M-mutant DIPG due to reversal of inadvertent activation of detrimental SE programs in comparison with monotherapy.