bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early detection of COVID-19 mortality risk using non-invasive clinical characteristics
Authors: Mahdavi Mahdi , Choubdar Hadi , Zabeh Erfan , Rieder Michael , Safavi-Naeini Safieddin , Khanlarzadeh Vida , Jobbagy Zsolt , Ghorbani Amirata , Abedini Atefeh , Kiani Arda , Lashgari Reza , Kamrani Ehsan 
Published: October 2020

Abstract
With no effective treatment currently available and maximum preventive measures already in place, more interventions in the clinical field are needed to decrease COVID-19 patient mortality. Early prediction of mortality risk in COVID-19 patients can decrease mortality by assuring efficient resource allocation and treatment planning. This study conducts an early prediction of COVID-19 prognosis using laboratory, clinical, and demographic data collected from patients in the first day of admission. Three machine learning models were developed to investigate and compare the prediction power of invasive and noninvasive biomarkers. The results suggest that early mortality prediction of patients via non-invasive biomarkers provides significant accuracy and can be used as a triage assisting tool without the need for
additional costs or waiting time of laboratory tests.
Development of EphA2 siRNA-loaded lipid nanoparticles and combination with a small-molecule histone demethylase inhibitor in prostate cancer cells and tumor spheroids
Authors: Oner Ezgi , Kotmakci Mustafa , Baird Anne-Marie , Gray Steven G. , Butuner Bilge Debelec , Bozkurt Emir , Kantarci Ayse Gulten , Finn Stephen P. 
Published: October 2020

Abstract
Background: siRNAs hold a great potential for cancer therapy, however, poor stability in body fluids and low cellular uptake limit their use in the clinic. To enhance the bioavailability of siRNAs in tumors, novel, safe, and effective carriers are needed. Results: Here, we developed cationic solid lipid nanoparticles (cSLNs) to carry siRNAs targeting EphA2 receptor tyrosine kinase (siEphA2), which is overexpressed in many solid tumors including prostate cancer (PCa). Using DDAB cationic lipid instead of DOTMA reduced nanoparticle size and enhanced both cellular uptake and gene silencing in PCa cells. DDAB-cSLN showed better cellular uptake efficiency with similar silencing compared to commercial transfection reagent Dharmafect-2. After verifying the efficacy of siEphA2-loaded nanoparticles, we further evaluated a potential combination with a histone lysine demethylase inhibitor, JIB-04. Silencing EphA2 by siEphA2-loaded DDAB-cSLN did not affect the viability (2D and 3D), migration, and clonogenicity of PC-3 cells alone. However, upon co-administration, there was a decrease in the aforementioned cellular responses due to JIB-04. Furthermore, JIB-04 decreased EphA2 expression, and thus, silencing efficiency of siEphA2-loaded nanoparticles was further increased with co-treatment. Conclusions: We have successfully developed a novel siRNA-loaded lipid nanoparticle for targeting EphA2. Moreover, detailed preliminary results of the effects of JIB-04, alone and in combination with siEphA2, on PCa cells and tumor spheroids were presented for the first time. Our delivery system provides high transfection efficiency and shows a great promise for targeting other genes and cancer types in further in vitro and in vivo studies.
The Physics of Detonation Chemistry: A Radical Theory in Predicting the Deflagration to Detonation Transition
Authors: Kumar V R Sanal , Chandrasekaran Nichith , Sankar Vigneshwaran , Sukumaran Ajith , Mani Sivabalan , kumar Tharikaa Ramesh , Padmanabhan Sathyan , Baskaran Roshan Vignesh , Natarajan Vishnu , Doddi Hema Sai Nagaraju , Nejaamtheen Mohammed Niyasdeen , Rafic Sulthan Ariﬀ Rahman Mohamed , Baskaran Roshan Vignesh , Murugesh Pavithra , Mariappan Amrith , K. Thianesh U. , moorthi Anand , Elumalai Mohan , R.S. Bharath , Oommen Charlie , Radhakrishnan Pradeep Kumar , Choudhary Shiv Kumar 
Published: October 2020

Abstract
The theoretical finding of the Sanal-flow-choking [PMCID: PMC7267099] is a methodological advancement in predicting the deflagration-to-detonation-transition (DDT) in the real-world-fluid flows (continuum/non-continuum) with credibility.[1,2] Herein, we provide a proof of the concept of the Sanal-flow-choking and streamtube-flow-choking causing DDT in wall-bounded and free-external flows. Once the streamlines compacted, the considerable pressure difference attains inside the streamtube and the flow gets accelerated to the constricted region for satisfying the continuity condition set by the conservation law of nature. If the shape of the streamtube in the internal/external flow is similar to the convergent-divergent (CD) duct the phenomenon of the Sanal-flow-choking and supersonic flow development occurs at a critical-total-to-static pressure ratio (CPR) in yocto to yotta scale systems and beyond, which leads to shock wave generation or detonation as the case may me. At the lower critical detonation or hemorrhage index, the CPR of the reacting flow and the critical blood-pressure-ratio (BPR) of the subjects (human being/animal) are unique functions of the heat-capacity-ratio (HCR) of the evolved gas in the CD duct. In silico results are presented herein to establish the proof of the concept of the Sanal-flow-choking and streamtube-flow-choking causing shock-wave/detonation in diabatic flow systems and asymptomatic-hemorrhagic-stroke in biological systems. The physics of detonation chemistry presented herein sheds light for exploring supernova explosions.[107]
Unilateral Pedicle Fracture Accompanying Spondylolisthesis and Contralateral Spondylolysis: A Case Report
Authors: Liu Xiaofeng , Baiketuerxun Palihati , Zhang Junxin , Liu Tao 
Published: October 2020

Abstract
Study design Case reportObjective To report a rare case with unilateral pedicle stress fracture accompanying spondylolytic spondylolisthesis. Summary of Background Data Unilateral pedicle stress fracture accompanying spondylolytic spondylolisthesis is rare. Most are association with major trauma, previous spine surgery, osteopetrosis or stress-related activities.Methods We report a patient with spontaneous pedicle fracture associated with contralateral spondylolysis and spondylolisthesis at the L5 level, complaining severe back pain, radicular lower limb pain and intermittent claudication. The pathophysiological mechanism is discussed, and a review of relevant literature is included.Results This patient was successfully treated by decompression laminectomy with transforaminal lumbar interbody fusion (TLIF) and pedicle screw fixation. To our knowledge, cases like this have rarely been reported in the literature.Conclusions Unilateral pedicle stress fracture in patients with spondylolytic spondylolisthesis is rare. It may be related to redistribution of forces in an unstable neural arch resulting from defect in the contralateral pars interarticularis. Our experience suggests that limited decompression and instrumented fusion surgery led to a successful outcome.
Identification of the ASPM-miR-26b-5p network associated with the aggressive traits of HCC cells
Authors: Li Hong-Guang , Gao Heng-Jun , Liu Fang-Feng , Liu Jun 
Published: October 2020

Abstract
Background: Even though earlier reports have revealed that abnormal spindle-like microcephaly associated (ASPM) exert essential roles in diverse malignancies, its relationship between specific microRNAs (miRNAs) in regulation of hepatocellular carcinoma (HCC) progression has never been elaborated. Methods: Bioinformatics analysis detected differentially expressed genes in HCC and normal. qRT-PCR was performed to detect expression of miR-26b-5p in HCC tissues and cells. HCC cells were transfected with plasmids and their proliferative ability and colony formation were detected with loss-of-function assay. The invasion of HCC cells was determined using Transwell assay. The expression of ASPM was detected by western blotting. Luciferase reporter gene assay was performed to detect the interaction between miR-26b-5p and ASPM. ASMP silencing cells were injected into mice to establish xenograft tumor model.Results: Herein, we proved that ASPM was upregulated in HCC and higher level of ASPM was significantly associated with worse survival in HCC patients. ASPM silencing restrained HCC cell proliferation, migration and invasion capacities in vitro. In vivo, downregulation of ASPM also suppressed HCC cells growth. Mechanistic analyses illustrated that ASPM was a directly target of miR-26b-5p. The expression of ASPM was negatively modulated by miR-26b-5p. Rescues assays displayed that miR-26b-5p inhibited HCC cells growth and invasion via modulating the expression of ASPM. Conclusions: Our work validated that miR-26b-5p restrained the aggressiveness of HCC cells through targeting ASPM.
Transcription Elongation Factor A-Like 7, regulated by miR-758-3p inhibits the malignant process of melanoma through decreasing the expression levels of c-Myc and AKT1
Authors: Liu Xilin , Song Xianji , Li Hong 
Published: October 2020

Abstract
Background: The ectopic expression of transcription elongation factor A (SII)-like 7 (TCEAL7) has been observed in several kinds of cancers, but its role in melanoma is still unclear. This study was carried out to investigate TCEAL7 role in melanoma progression, and uncover the underlying mechanisms. Methods: TCEAL7 expression level in melanoma tissues and cells were determined by using RT-PCR and western blotting. CCK-8, transwell chambers, flow cytometry, starch assay and tumorigenesis assay were applied to detect cell growth, invasion, apoptosis, migration and tumorigenesis, respectively. Results: A low expression level of TCEAL7 was observed in melanoma tissues and cells, which associated with malignant process and poor prognosis. TCEAL7 negatively modulated AKT1, AKT2 and c-Myc expression and inhibited cancer progression via decreasing AKT1 and c-Myc expression. In addition, TCEAL7 was negatively modulated by miR-758-3p which promoted melanoma progression. Moreover, TCEAL7 overexpression abolished miR-758-3p-mediated melanoma progression. Conclusion: This study demonstrated that TCEAL7, regulated by miR-758-3p inhibited the malignant process of melanoma through decreasing the expression levels of c-Myc and AKT1.
CD44, A Marker of Cancer Stem Cells, is Positively Correlated With PD-L1&nbsp;Expression and Immune Cells Infiltration in Lung Adenocarcinoma
Authors: Zhang Chenyue , Wang Hui , Wang Xia , Zhao Chenglong , Wang Haiyong 
Published: October 2020

Abstract
Background: PD-L1 inhibitors is widely applied in lung adenocarcinoma patients. Tumor cells with high PD-L1 expression could trigger immune evasion. Cancer stem cells (CSCs) can evade from immunesurveillance due to their immunomodulating effects. However, the correlation between CSC and PD-L1 and some immune-related markers is seldom reported in patients with lung adenocarcinoma. Therefore, we aimed to ascertain their association in lung adenocarcinoma patients. Methods: We assessed CD44 expression and its association with PD-L1 in lung adenocarcinoma, using Tumor Immune Estimation Resource (TIMER), which was further validated in our patient cohort. The immune cells infiltration was depicted by CIBERSORT using GEO database. The correlation between CD44 and immune cells was also analyzed. We further evaluated the prognostic role of CD44 in patients with lung adenocarcinoma both using Kaplan-Meier plotter and validated in our patient cohort. Results: Positive association between CD44 and PD-L1 were found in lung adenocarcinoma patients. T cells CD4 memory resting cells and mast cells resting cells varied significantly between patients with CD44 high and those with CD44 low. Furthermore, positive association could be found between CD44 expression and immune cells. Arm-level depletion of CD44 was linked with B cell, CD4+ T cell, neutrophil and dendritic cell infiltration. Patients with higher CD44 levels had worsened overall survival (OS). Conclusions: In summary, these results demonstrate that CD44 was associated with PD-L1 and infiltration of immune cells, and was a negative prognostic factor for predicting worsened OS in lung adenocarcinoma.
A Tall and Thin Boy with a Bad Headache:&nbsp;A Case Report of Homocystinuria
Authors: Wiel Luisa Cortellazzo , Caddeo Giulia , Zanchi Chiara , Gortani Giulia , Faletra Flavio , Bruno Irene , Magnolato Andrea , Barbi Egidio 
Published: October 2020

Abstract
Background: Homocystinuria is a rare inborn error of metabolism, resulting in the accumulation of homocysteine and methionine in tissues, which manifests as disorders of the connective tissues and increased risk of thromboembolism. The latter is the primary cause of morbidity and mortality, and can be prevented by the prompt start of the specific therapy aimed at lowering homocysteine plasma levels.Case presentation: A nine-year-old boy was admitted to the Emergency Department for a severe headache associated with torticollis, dysarthria, difficulty in walking and extreme irritability. His past medical history was relevant for bilateral lens ectopia and epilepsy, associated with a “marfanoid habitus”. A brain CT scan was consistent with cerebral venous thrombosis. The association between the marfanoid habitus and the thromboembolic event raised the suspicion of homocystinuria, which was eventually confirmed by the high levels of serum homocysteine, and the genetic testing. Anticoagulation therapy was started, together with pyridoxine and folate, and eventually betaine and dietary protein restriction, with a consequent satisfactory fall in homocysteine levels, along with clinical recovery.Conclusions: This case enhances the importance of dosing serum homocysteine in every child investigated for a marfanoid habitus, given the impact of the early diagnosis and treatment in the prevention of thromboembolic events.
Clinical Characteristics and Outcomes of Tuberculosis Infection in Adult Patients with MDS
Authors: Ren Yanling , Mei Chen , Ye Li , Zhou Xinping , Xu Gaixiang , Ma Liya , Lu Chenxi , Wang Lu , Yang Haiyang , Luo Yingwan , Zhu Shuanghong , Jiang Lingxu , Jin Jie , Tong Hongyan 
Published: October 2020

Abstract
Background and aim Despite the high incidence of tuberculosis infection (TBI) in patients with hematological diseases, there were few researches on TBI in MDS patients. We retrospectively analyzed the characteristics of MDS patients with TBI in our center to improve the clinical diagnosis and treatment of these patients.Methods Primary MDS patients diagnosed between 2014 and 2019 at the First Affiliated Hospital of Zhejiang University were retrospectively reviewed, TBI was diagnosed by positive culture(s) or acid fast bacilli on modified Ziehl-Neelsen staining on clinical samples as well as compatible symptoms and signs.Results Thirty-four of 1347 MDS patients were diagnosed with TBI, nine patients had blast＜5%, twelve patients were MDS with excess blast-1(MDS-EB1), and the rest 13 patients were MDS with excess blast-2 (MDS-EB2). Ten patients had lower risk (low and intermediate) and 24 patients had higher risk (high and very high) according to revised international scoring system (IPSS-R). Moreover, fifteen patients were classified into synchronous group (defined as TBI occurring within 3 months of MDS diagnosis), and the rest 19 patients were non-synchronous group (defined as TBI developed after MDS more than 3 months). Patients in synchronous group have higher rate of persistent fever (11/15 vs. 7/19, p=0.03), higher risk classification ( 14/15 vs. 8/19, P=0.002 ), and higher tendency to transform to acute myeloid leukemia (AML) (8/15 vs. 4/19, P=0.04 ) than those in non-synchronous group. The median time to AML was significantly shorter in synchronous group (7.68 months vs. 23.83 months, P<0.01). Most patients received regular anti-tuberculosis treatment (ATT)(33/34) and support care(22/34) for MDS, patients received support care were more prone to proceed to AML than those received active therapy such as decitabine (12/22 vs. 2/12, P=0.03). The median OS of all patients in synchronous group was just 14.17 months, while in non-synchronous group, it was 34.07 moths ( P = 0.01). However, treatments for MDS had no effect on OS(P=0.67).Conclusion TBI is not uncommon among patients with MDS, patients with synchronous TBI and MDS progress faster and have shorter OS, which may be associated with genetic susceptibility and lack of active therapy.
Comprehensive Analysis of lincRNA-Related ceRNA Network in Glioblastoma
Authors: Liu Guangdong , Liu Danian , Huang Jingjing , Li Jianxin , Wang Chuang , Liu Guangyao , Ge Shiqiang , Gong Haidong 
Published: October 2020

Abstract
BackgroundLong intergenic non-coding RNAs (lincRNAs) are capable of regulating several tumours, while competitive endogenous RNA (ceRNA) networks are of great significance in revealing the biological mechanism of tumours. Currently, there is a dearth of studies on the ceRNA network of lincRNAs in glioblastoma (GBM), which we aimed to assess in the present study. MethodsWe obtained GBM and normal brain tissue samples from TCGA, GTEx, and GEO databases, and performed weighted gene co-expression network analysis and differential expression analysis on all lincRNA and mRNA data. Subsequently, we predicted the interaction between lincRNAs, miRNAs, and target mRNAs. Univariate and multivariate Cox regression analyses were performed on the mRNAs using CGGA data, and a Cox proportional hazards regression model was constructed. ResultsAccording to the Cox model, we assembled a ceRNA network consisting of 23 lincRNAs, 14 miRNAs, and 13 mRNAs. Gene Set Enrichment Analysis was carried out on four lincRNAs with obvious differential expressions and relatively few studies in GBM. ConclusionWe identified four lincRNAs that have the most research values for GBM and obtained the ceRNA network. Our research is expected to facilitate in-depth understanding and study of the molecular mechanism of GBM, and provide new insights into targeted therapy and prognosis of the tumour.
​Short-term changes in and preoperative factors affecting vaulting after posterior chamber phakic Implantable Collamer Lens implantation
Authors: Zhu Qiujian , Chen Wen-Jing , Zhu Wei-Jian , Xiao Hai-Xiang , Zhu Man-Hui , Ma Lie , Yuan You , Song E. 
Published: October 2020

Abstract
Background: To describe the very early changes in vault in the first month after Implantable Collamer Lens (ICL) implantation and to evaluate the effect of preoperative biometric factors on vault.Methods: A total of 83 eyes from 83 subjects with complete data who met follow-up requirements were recruited in this retrospective study between May 2019 and March 2020. We quantitatively assessed postoperative vault at 2 hours, 1 day, 1 week, and 1 month following implantation. Associations between postoperative vault and age, ICL size, spherical equivalent (SE), axial length (AL), central corneal thickness (CCT), flat K, steep K, mean K, anterior chamber depth (ACD), crystalline lens thickness (LT), white-to-white (WTW) diameter obtained by three devices, horizontal and vertical sulcus-to-sulcus (STS) diameter, bright and dark pupil sizes (BPS and DPS) and DPS-BPS were investigated using Spearman’s correlation analysis and stepwise multiple regression analysis.Results: The mean vault values at 2 hours, 1 day, 1 week, and 1 month after ICL implantation were 672.05 ± 30.72, 389.15 ± 28.33, 517.23 ± 30.76 and 530.12 ± 30.22 μm, respectively. There were significant differences in vault values between 2 hours, 1 day and 1 week after the operation. ICL size (β = 0.942; p < 0.001), followed by horizontal STS (β = -0.517; p < 0.001), crystalline LT (β = -0.376; p < 0.001) and vertical STS (β = -0.257; p = 0.017), significantly influenced vault at 1 month after the operation. The multiple regression equation was expressed as follows: central vault (μm) = -1369.05+ 657.121 × ICL size- 287.408 × horizontal STS - 432.497 × crystalline LT - 137.33 × vertical STS (adjusted R2 = 0.643).Conclusions: After ICL implantation, vault decreased and then increased, but it did not return to the vault value at 2 hours after surgery. ICL size, horizontal and vertical STS and crystalline LT were key factors for predicting postoperative vaulting.
Factors Affecting Final Functional Outcomes in Open-Globe Injuries and Use of Ocular Trauma Score in Nepalese population
Authors: Shrestha Saurav Man , Anthony Casey Leigh , Grant Justin A. , Thapa Madhu , Shrestha Jyoti Baba , Khatri Anadi , Hoskin Annette K. , Agrawal Rupesh 
Published: October 2020

Abstract
Background: Open globe injury (OGI) is one of the most devastating form of ocular trauma. The aim of the study is to identify the epidemiology and predict visual outcomes in traumatic open globe injuries using ocular trauma score (OTS) and correlate with final visual acuity (VA) at 3 months. Methods: Patients older than 5 years, presenting to B.P. Koirala Lions Centre for Ophthalmic Studies (BPKLCOS) from March 2016- March 2017 with OGI that met inclusion criteria were evaluated. Patient profile, nature and cause of injury, and time to presentation were recorded. Patients were managed accordingly and followed up to 3 months. An OTS score for each patient was calculated and raw scores were categorized accordingly. The VA after 3 months were compared to the predicted OTS values. Results: Seventy-three eyes of 72 patients were examined. 76% were male, and the mean age was 26.17 years (median, 23.5 years). The mean time from injury to presentation was <6 hours (30 patients, 41%). Thirty-seven eyes (51%) had zone I trauma, followed by twenty eyes (27%) with zone II, and sixteen eyes (22%) with zone III trauma. Sixty-five patients (90%) were managed surgically, and fifty (68%) received intravitreal antibiotics with steroid. When compared, the projected VA as per OTS were able to predict actual final visual outcomes in 60% of the eyes with OGI of various zones (p<0.05). Conclusion: OTS can be an accurate predictive tool for final visual acuity even with a short follow up period of 3 months; with poor presenting visual acuity, delayed presentation, posterior zones of injury, need for intravitreal injections, endophthalmitis, and globe rupture associated with poorer prognosis.
Failure of labor induction in Tigray region, Ethiopia: a retrospective record review study
Authors: Abraha Teklehaymanot Huluf , Kasahun Wondwossen Wollie , Fisseha Gezienesh Kahsay 
Published: October 2020

Abstract
Background: Failed induction of labour is defined as when there is failure to generate regular contractions (i.e, every 3 minute) and cervical change after artificial membrane rupture and at least 24 hour of oxytocin use However, the magnitude of failed induction labor in Tigray is not well documented. Therefore, the objective of this study was to assess the magnitude and factors associated with failed induction of labor in the study area. Methods: We conducted institution-based- retrospective record review study of all inductions of labor conducted from April 9, 2017- April 8, 2018(n=380) in Axum St.Mary hospital, Tigray, Ethiopia. Data were entered and analyzed using SPSS version 21 software. The data were cleaned before analysis. Descriptive statistics such as frequency and mean were used to describe the study participants. For the multivariable logistic, binary logistic regression a two tailed p-value <0.05 was performed to show statistical significant. Results: The magnitude of failure of labor induction was found 11.8% (95% CI: 8.7-15.3%). The multivariable binary logistic regression analysis showed that primi-parity (AOR=4.31, 95% CI; 1.22-15.18) and poor Bishop Score (AOR=10.67, 95%CI: 1.31-86.87) were significantly associated with failed induction of labor. Conclusion and recommendation: The failed of labor induction was found high in the study area. The health care providers should assess and document the status of the cervix (using the Bishop score) to determine the probability of success and to select the appropriate method of induction. In addition, they need to consider induction of women with an unfavorable cervix is associated with high failure and a higher caesarean section.