bluuebunny/crossref_metadata_2025_split huggingface dataset:
TUG1 long non‐coding RNA enlists the USF1 transcription factor to overexpress ROMO1 leading to hepatocellular carcinoma growth and metastasis
Authors: Liu Shihai , Qiu Jing , He Weitai , Geng Chao , He Guifang , Liu Changchang , Cai Duo , Liu Xiangping , Tian Ben , Pan Huazheng 
Published: November 2020

AbstractHepatocellular carcinoma (HCC) is a prevalent and highly aggressive cancer. Long non‐coding RNAs (lncRNAs) are recognized as potential molecular targets for HCC and are currently under increased research focus. Here, we investigate the regulatory processes underlying the axis of the lncRNA taurine upregulated gene 1 (TUG1), Upstream Transcription Factor 1 (USF1), and reactive oxygen species modulator 1 (ROMO1) in the propagation and metastasis of HCC cells. Distribution of lncRNA TUG1 was found to be prominent in HCC cell cytoplasm and nuclei. LncRNA TUG1 conscripted the USF1 transcription factor to enhance the promoter function of ROMO1. Enlisting the USF1 transcription factor to increase ROMO1 expression following upregulation of TUG1 lncRNA enhanced HCC Huh7 cell proliferation, motility, and metastasis. Rapid tumor proliferation in nude mice provided in vivo verification. The importance of the lncRNA TUG1/USF1/ROMO1 complex as a target for HCC therapy is a key result of this investigation which is exemplified by its role in regulating the proliferation, motility, and metastasis of HCC cells.
Downregulation of gene expression and the outcome of ICSI in severe oligozoospermic patients: A preliminary study
Authors: Sadakierska‐Chudy Anna , Patrylak J. , Janeczko J. , Chudy J. 
Published: November 2020

AbstractPreimplantation embryo development might be influenced by a specific set of transcripts that are delivered to the oocyte by the sperm. The aim of the study was to determine the relationship between the level of selected transcripts in spermatozoa and preimplantation development of the embryos in couples with severe oligozoospermia undergoing intracytoplasmic sperm injection (ICSI) procedure. Therefore, we assessed messenger RNA (mRNA) levels of genes involved in fertilization events, oocyte activation, chromatin remodeling, and DNA repair in severe oligozoospermic compared with normozoospermic men as well as morphokinetic parameters of embryos using the time‐lapse imaging system. mRNA profiling (44 genes), in mature sperm, was carried out with custom‐designed 384‐well TLDA Cards. The morphokinetic parameters of zygotes and embryos were recorded by using a time‐lapse imaging system. The transcript levels of 21 genes were significantly decreased in the severe oligozoospermic group. Most were associated with fertilization events, oocyte activation and embryonic genome activation. Among them, mRNA of AKAP4 and PTK7 was greatly reduced, moreover, the transcripts of PLCζ and POU5F1, essential for OA and EGA, were not detected at all in patients with severe oligozoospermia. Moreover, the reduced expression of genes important for spermatogenesis, chromatin remodeling and DNA repair was also observed in this group. Time‐lapse analysis revealed that fertilization failure occurred in 14% of retrieved oocytes and 90% of all degenerated embryos did not reach morula stage. This study provides preliminary results indicating a significant decrease in transcripts of genes important for spermatogenesis and early preimplantation development in the mature sperm of men with severe oligozoospermia.
A feasibility study of an integrated couples‐based supportive programme for Chinese couples living with colorectal cancer
Authors: Luo Xingjuan , Li Jieyu , Cao Qian , Sun Liya , Chen Ying , Zhao Jie , Li Qiuping 
Published: November 2020

AbstractAimTo test the feasibility, acceptability and preliminary efficacy of using an integrated approach combined online and in‐person delivery to support colorectal cancer (CRC) patients and their spousal caregivers coping positively with cancer together.DesignA single‐arm pre–post‐feasibility design was used.MethodsChinese CRC patient‐partner dyads (N = 24) accessed the blended intervention combined online platform and face‐to‐face sessions for six weeks between October 2019 to January 2020. Feasibility was measured through recruitment and retention and acceptability was examined by intervention engagement and post‐treatment programme evaluation. Effect sizes were calculated using the complete data (N = 20 couple dyads) to evaluate preliminary treatment effect.ResultsRecruitment (70.6%) and retention rates (83.3%) supported programme feasibility. Participants’ positive intervention engagements and evaluations indicated acceptability. The overall small‐to‐medium improvements in most outcome measures verified preliminary efficacy of the integrated couples‐based supportive programme. The findings support its feasible and acceptable for couples coping with CRC and show potential efficacy.
Teaching bioscience to nursing students—What works?
Authors: Knutstad Unni , Småstuen Milada Cvancarova , Jensen Kari Toverud 
Published: November 2020

AbstractAimTo compare the effects of flipped classroom and traditional auditorium lectures, on nursing students’ examination results in bioscience.DesignAn educational intervention study.MethodsAll the first‐year students in the bachelor programme (N = 493) were entered into a database and randomly assigned to the intervention or the control group in a course in bioscience. The outcome measures are the proportion of students who passed the examination, and the distribution of grades from A to E. Chi‐square tests and Mann–Whitney Wilcoxon test were used. The odds to pass versus fail were modelled using binary logistic regression.ResultsThe proportion of students who did not pass the final examination was very similar in the intervention and the control groups, 21.4% and 23.6% (p = .574). Our data did not reveal any statistically significant differences concerning the distribution of grades (p = .691). Students with biology and/or natural science had higher odds for passing.
Extracardiac Doppler indices predict perinatal mortality in fetuses with Ebstein anomaly and tricuspid valve dysplasia
Authors: Wang Shuo , Freud Lindsay R. , Detterich Jon , Moon‐Grady Anita J. , Donofrio Mary T. , Jaeggi Edgar T. , Szwast Anita L. , Morris Shaine A. , Kavanaugh‐Mchugh Ann , Howley Lisa W. , van der Velde Mary E. , Cuneo Bettina F. , Phoon Colin K. , Tworetzky Wayne , Pruetz Jay D. 
Published: November 2020

AbstractObjectivesEbstein anomaly and tricuspid valve dysplasia (EA/TVD) carry high perinatal mortality. Past studies have focused on cardiac predictors of mortality; we sought to describe the fetal echo (FE) extracardiac Dopplers in this cohort and determine their association with perinatal mortality.MethodFetuses with EA/TVD at 23 centers from 2005–2011 were included for retrospective study. Doppler pattern and velocity of the umbilical artery (UA), umbilical vein (UV), ductus venosus (DV), and middle cerebral artery (MCA) were collected. Bivariate and multivariate analyzes were performed. The primary outcome measure was perinatal mortality, defined as fetal demise or neonatal death.ResultsOf 190 cases that met eligibility criteria, alterations were seen in 50% of UA, 16% of UV, 48% of DV, and 8% of MCA Doppler indices on the last FE (median 27.4 weeks). Independent predictors of perinatal mortality included abnormal UA Doppler pattern of absence or reversed end diastolic flow (OR 9.7) and UV velocity z score <1 (OR 2.5), in addition to diagnosis <32 weeks (OR 4.2) and tricuspid valve (TV) annulus z score ≥6 (OR 5.3).ConclusionAbnormal UA Doppler pattern and decreased UV velocity are independent predictors of perinatal mortality in EA/TVD fetuses and should be used to refine mortality risk and guide perinatal management.
Study of psychological state of cancer patients undergoing radiation therapy during novel coronavirus outbreak and effects of nursing intervention
Authors: Lou Shuchang , Xu Dejing , Li Xiaodong , Huan Yan , Li Jun 
Published: November 2020

AbstractObjectiveTo investigate psychological state of hospitalized cancer patients undergoing radiation therapy and evaluate effects of customized psychological intervention on patients' psychological state during novel Coronavirus (COVID‐19) outbreak.MethodFifty‐eight hospitalized head and neck cancer patients undergoing radiation therapy were included and received online and offline psychological intervention. General information questionnaire and the Self‐Report Symptom Inventory, Symptom Check‐List90 (SCL‐90) were utilized to investigate and analyze psychological state of hospitalized head and neck cancer patients undergoing radiation therapy before and after intervention. Self‐Rating Depression Scale (SDS) and Self‐Rating Anxiety Scale (SAS) were used to evaluate depression severity and anxiety severity of them.ResultsOverall psychological state of patients (include nine symptom dimensions: somatization, obsessive, interpersonal sensitivity, depression, anxiety, hostility, phobic anxiety, paranoid ideation, and psychoticism) was improved significantly after intervention (P < .05). Moreover, scores of SAS and SDS were lowered.ConclusionCustomized psychological intervention helped to improve overall psychological state of hospitalized cancer patients undergoing radiation therapy during COVID‐19 outbreak and showed encouraging effects on reduction severity of depression and anxiety.
Next generation sequencing based detection of 15 target genes mutations in papillary thyroid carcinoma
Authors: Wang Zhuo , Jing Changwen , Cao Haixia , Liu SiWen , Wu Jianzhong , Ma Rong 
Published: November 2020

AbstractObjectivesNext generation sequencing (NGS) method provided a valuable tool for a comprehensive understanding of papillary thyroid carcinoma (PTC) biology. We explored the application of NGS based detection for a panel of 15 target genes mutations and analyzed the gene spectrum in PTC.MethodsA total of 211 formalin‐fixed, paraffin‐embedded (FFPE) tissue specimens from surgically removed PTC samples were collected and detected with 15 target genes by NGS.ResultsIn general, 181 mutations of all types of 15 target genes were detected in 164 samples (77.73% of 211 samples). There were 22.27% samples with no mutation, and 70.14% samples carried mutations in single gene. A total of 7.11% samples simultaneously harboured two gene mutations and 0.47% sample carried triple different gene mutations. The BRAF mutation was the most common mutation type, followed by TERT, RET fusion, TP53, PIK3CA, GNAS, NTRK1 fusion, CTNNB1, NRAS, and HRAS. TSHR, AKT1, PETN, KRAS, and PAX8 mutations were negative among PTC patients. All point mutations found in PTC samples were heterozygous mutations with allelic frequency ranged from 1.12% to 48.04% of alleles. The fusion mutations showed the percent of reads ranged from 2.30% to 55.54%. For samples with carried two mutations, the allelic frequency of mutations was similar. However, for the sample harboured triple gene mutations, the allelic frequency of NRAS mutation was similar to TERT mutation (33.62%‐39.67%) and TP53 mutation showed much lower abundance (4.13%).ConclusionsNGS‐based detection method showed advantages in detecting multiple gene mutations simultaneously, economically and efficiently, and providing quantitative assessment of mutation frequency using targeted sequencing panel. It can improve the accuracy of diagnosis and prognostication and is useful for providing personalized treatments for PTC patients.
‘Light‐Up’ AIE‐Active Materials: Self‐Assembly, Molecular Recognition and Catalytic Applications
Authors: Kaur Mandeep , Kaur Harpreet , Kumar Manoj , Bhalla Vandana 
Published: November 2020

AbstractAggregation induced emission enhancement (AIEE) is one of the most widely explored phenomena to develop ‘light up’ (fluorescent) materials having potential applications in the field of supramolecular chemistry, analytical chemistry and material chemistry. By applying the principles of host‐guest chemistry, we have developed a variety of aggregation induced emission (AIE/AEE) active materials having specific affinity for metal ions, electron deficient/electron rich analytes. The interactions between AIE active assemblies and metal ions are further tuned to prepare nanohybrids having potential applications as catalytic/photocatalytic systems in various organic transformations under eco‐friendly conditions. This account summarizes various design strategies developed in our labortary for the preparation of AIE/AEE active building blocks having sensing and catalytic applications.
Chiral N‐tert‐Butylsulfinyl Imines: New Discoveries
Authors: Foubelo Francisco , Yus Miguel 
Published: November 2020

AbstractIn this account the reactions of chiral N‐tert‐butylsulfinyl imines with organometallic reagents such as organoalkaline (lithium, sodium, potassium and cesium derivatives), organomagnesium, organozinc, organoboron, organoaluminium, organoindium and organosilicon compounds is comprehensively described. The reactivity in all cases is derived to synthetic applications in order to prepare interesting organic nitrogenated molecules, especially in the field of alkaloid compounds.
Coordination Self‐Assembly Processes Revealed by Collaboration of Experiment and Theory: Toward Kinetic Control of Molecular Self‐Assembly
Authors: Hiraoka Shuichi , Takahashi Satoshi , Sato Hirofumi 
Published: November 2020

AbstractThe importance of the collaboration of experiment and theory has been proven in many examples in science and technology. Here, such a new example is shown in the investigation of molecular self‐assembly process, which is a complicated multi‐step chemical reaction occurring in the reaction network composed of a huge number of intermediates. An experimental method, QASAP (quantitative analysis of self‐assembly process), developed by us and a numerical approach, NASAP (numerical analysis of self‐assembly process), that analyzes the experimental data obtained by QASAP to draw detail molecular self‐assembly pathways, which was also developed by us, are introduced, and their application to the investigation of Pd(II)‐mediated coordination assemblies are presented. Further, the possibility of the prediction of the outcomes of molecular self‐assembly by varying the reaction conditions is also demonstrated. Finally, a future direction in the field of artificial molecular self‐assembly based on pathway‐dependent self‐assembly, that is, kinetic control of molecular self‐assembly is discussed.
Synthesis of β‐Lactams and β‐Homoprolines by Fragmentative Rearrangement of 5‐Spirocyclopropaneisoxazolidines Mediated by Acids
Authors: Cordero Franca M. , Brandi Alberto 
Published: November 2020

AbstractAzetidinones and β‐amino acids serve as useful building blocks in synthetic organic chemistry and their structural motifs are often found in biologically active compounds. Due to the importance of these compounds, several synthetic strategies have been developed and availability of new synthetic approaches is highly desirable. In this account, we describe the development of an original method that allows the preparation of β‐lactam and β‐homoproline derivatives not easily accessible through traditional processes. The serendipitous discovery made in our lab in 2000 involved the formation of a β‐lactam by heating a mixture of an alkylidenecyclopropane tethered to a formyl group with N‐methylhydroxylamine hydrochloride. Investigation of the process resulted in disclosing an alternative synthetic method of azetidinones based on an acid induced fragmentative rearrangement of cycloadducts of nitrones with suitable methylenecyclopropane derivatives. Herein, the scope of this process is reviewed. In addition, both experimental and computational studies of the mechanism for this peculiar fragmentative rearrangement are presented.
Targeting abnormal metabolism in Alzheimer's disease: The Drug Repurposing for Effective Alzheimer's Medicines (DREAM) study
Authors: Desai Rishi J. , Varma Vijay R. , Gerhard Tobias , Segal Jodi , Mahesri Mufaddal , Chin Kristyn , Nonnenmacher Edward , Gabbeta Avinash , Mammen Anup M. , Varma Sudhir , Horton Daniel B. , Kim Seoyoung C. , Schneeweiss Sebastian , Thambisetty Madhav 
Published: November 2020

AbstractDrug discovery for disease‐modifying therapies for Alzheimer's disease and related dementias (ADRD) based on the traditional paradigm of experimental animal models has been disappointing. We describe the rationale and design of the Drug Repurposing for Effective Alzheimer's Medicines (DREAM) study, an innovative multidisciplinary alternative to traditional drug discovery. First, we use a systems biology perspective in the "hypothesis generation" phase to identify metabolic abnormalities that may either precede or interact with the accumulation of ADRD neuropathology, accelerating the expression of clinical symptoms of the disease. Second, in the "hypothesis refinement" phase we propose use of large patient cohorts to test whether drugs approved for other indications that also target metabolic drivers of ADRD pathogenesis might alter the trajectory of the disease. We emphasize key challenges in population‐based pharmacoepidemiologic studies aimed at quantifying the association between medication use and ADRD onset and outline robust causal inference principles to safeguard against common pitfalls. Candidate ADRD treatments emerging from this approach will hold promise as plausible disease‐modifying therapies for evaluation in randomized controlled trials.
POD‐based analysis of a wind turbine wake under the influence of tower and nacelle
Authors: De Cillis Giovanni , Cherubini Stefania , Semeraro Onofrio , Leonardi Stefano , De Palma Pietro 
Published: November 2020

AbstractThe wake produced by a model wind turbine is investigated using proper orthogonal decomposition (POD) of numerical data obtained by large eddy simulations at a diameter‐based Reynolds number
. The blades are modeled employing the actuator line method and an immersed boundary method is used to simulate tower and nacelle. Two simulations are performed: one accounts only for the blades effect; the other includes also tower and nacelle. The two simulations are analyzed and compared in terms of mean flow fields and POD modes that mainly characterize the wake dynamics. In the rotor‐only case, the most energetic modes in the near wake are composed of high‐frequency tip and root vortices, whereas in the far wake, low‐frequency modes accounting for mutual inductance instability of tip vortices are found. When tower and nacelle are included, low‐frequency POD modes are present already in the near wake, linked to the von Karman vortices shed by the tower. These modes interact nonlinearly with the tip vortices in the far wake, generating new low‐frequency POD modes, some of them lying in the frequency range of wake meandering. An analysis of the mean kinetic energy (MKE) entrainment of each POD mode shows that tip vortices sustain the wake mean shear, whereas low‐frequency modes contribute to wake recovery. This explains why tower and nacelle induce a faster wake recovery.
Symmetry resolved entanglement in integrable field theories via form factor bootstrap
Authors: Horváth Dávid X. , Calabrese Pasquale 
Published: November 2020

AbstractWe consider the form factor bootstrap approach of integrable field theories to derive matrix elements of composite branch-point twist fields associated with symmetry resolved entanglement entropies. The bootstrap equations are determined in an intuitive way and their solution is presented for the massive Ising field theory and for the genuinely interacting sinh-Gordon model, both possessing a ℤ2symmetry. The solutions are carefully cross-checked by performing various limits and by the application of the ∆-theorem. The issue of symmetry resolution for discrete symmetries is also discussed. We show that entanglement equipartition is generically expected and we identify the first subleading term (in the UV cutoff) breaking it. We also present the complete computation of the symmetry resolved von Neumann entropy for an interval in the ground state of the paramagnetic phase of the Ising model. In particular, we compute the universal functions entering in the charged and symmetry resolved entanglement.
Accounting for the Heisenberg and Pauli principles in the kinetic approach to neutrino oscillations
Authors: Kartavtsev A. 
Published: November 2020

Abstract
While oscillations of solar neutrinos are usually studied using the single-particle quantum-mechanical approach, flavor conversions of supernovae neutrinos are typically analyzed using the kinetic equation for the matrix of densities due to the necessity of including also the scattering processes. Using the Wigner formulation of quantum mechanics we show the equivalence of the quantum-mechanical and kinetic approaches in the limit of collision-less neutrino propagation (in a background medium). Based on this observation we also argue that solutions of the kinetic equation account for the Heisenberg uncertainty principle and the related effect of wave packet separation (for single neutrinos), as well as the Pauli exclusion principle, if the initial conditions are consistent with these fundamental quantum principles. Such initial conditions can be constructed e.g. by identifying the matrix of densities with the (reduced) single-particle Wigner function computed using initial conditions for the neutrino wave function. Hence the neutrino momentum uncertainty is an integral part of the initial conditions for the matrix of densities, that may have an impact on the phenomenology of supernovae neutrinos via the effect of wave packet separation.
Fully exclusive heavy quark-antiquark pair production from a colourless initial state at NNLO in QCD
Authors: Somogyi Gábor , Tramontano Francesco 
Published: November 2020

Abstract
We present a local subtraction scheme for computing next-to-next-to-leading order QCD corrections to the production of a massive quark-antiquark pair from a colourless initial state. The subtraction terms are built following the CoLoRFulNNLO method and refined in such a way that their integration gives rise to compact, fully analytic expressions. All ingredients necessary for a numerical implementation of our subtraction scheme are provided in detail. As an example, we calculate the fully differential decay rate of the Standard Model Higgs boson to massive bottom quarks at next-to-next-to-leading order accuracy in perturbative QCD.