bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceived and Actual Growth Following Hematopoietic Stem-Cell Transplantation: Longitudinal Evidence of Greater Decline than Growth
Authors: Corman M. , Rubio M.-T. , Cabrespine A. , Brindel I. , Bay J.-O. , Tour Peffault De La , Dambrun Michael 
Published: August 2020

Abstract
Background. This prospective longitudinal study examined perceived and actual changes in post-traumatic growth following Hematopoietic Stem-Cell Transplantation (HSCT) and their relationships with mental health and psychological disposition. We also tested the hypothesis that unwillingness to be in contact with distressing thoughts and feelings—i.e. experiential avoidance—would moderate the relationship between Post-Traumatic Stress Disorder (PTSD) and growth.Methods. This study was carried out with 187 patients. All patients completed the Post-Traumatic Growth Inventory (PTGI) five months after HSCT and also scales tapping into the five domains of PTGI during and 5 months after HSCT. Mental health and psychological disposition were also assessed prior to hospitalization. A PTSD scale was administrated at the five-month follow-up.Results. Perceived and actual change were weakly correlated. Bayesian pre/post-HSCT comparisons in actual growth revealed substantial to very strong decline in four of the five dimensions assessed. Overall, RCI indicated a reliable increase for 5.6% of patients and a reliable decrease for 40.8% of patients. Confirming that perceived and actual growth reflect different processes, they were not related to the same mental health and psychological disposition variables. Moreover, the hypothesis that acquiring positive outcomes from a traumatic event, such as HSCT, requires direct confrontation with the source of distress that was supported in the case of perceived growth but not in the case of actual growth.Conclusions. Retrospective measures such as the PTGI do not appear to assess actual pre- to post-HSCT change. HSCT seems more linked to psychological decline than to growth.
Bioinformatic Analysis Revealing Mitotic Spindle Assembly regulated NDC80 and MAD2L1 as Prognostic Biomarkers in Non-Small Cell Lung Cancer Development
Authors: Wei Rong , Wang Ziyue , Zhang Yaping , Wang Bin , Shen Ningning , E Li , Li Xin , Shang Lifang , Shang Yangwei , Yan Wenpeng , Zhang Xiaoqin , Ma Wenxia , Wang Chen 
Published: August 2020

Abstract
Background Lung cancer has been the leading cause of tumor related death, and 80%~85% of it is non-small cell lung cancer (NSCLC). Even with the rising molecular targeted therapies, for example EGFR, ROS1 and ALK, the treatment is still challenging. The study is to identify credible responsible genes during the development of NSCLC using bioinformatic analysis, developing new prognostic biomarkers and potential gene targets to the disease. Methods Firstly, three genes expression profiles GSE44077, GSE18842 and GSE33532 were picked from Gene Expression Omnibus (GEO) to analyze the genes with different expression level (GDEs) between NSCLC and normal lung samples, and the cellular location, molecular function and the biology pathways the GDEs enriched in were analyzed. Then, gene function modules of GDEs were explored based on the protein-protein interaction network (PPI), and the top module which contains most genes was identified, followed by containing genes annotation and survival analysis. Moreover, multivariate cox regression analysis was performed in addition to the Kaplan meier survival to narrow down the key genes scale. Further, the clinical pathological features of the picked key genes were explored using TCGA data. Results Three GEO profiles shared a total of 664 GDEs, including 232 up-regulated and 432 down-regulated genes. Based on the GDEs PPI network, the top function module containing a total of 69 genes was identified, and 31 of 69 genes were mitotic cell cycle regulation related. And survival analysis of the 31 genes revealed that 17/31 genes statistical significantly related to NSCLC overall survival, including 4 spindle assembly checkpoints, namely NDC80, BUB1B, MAD2L1 and AURKA. Further, multivariate cox regression analysis identified NDC80 and MAD2L1 as independent prognostic indicators in lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) respectively. Interestingly, pearson correlation analysis indicated strong connection between the four genes NDC80, BUB1B, MAD2L1 and AURKA, and their clinical pathological features were addressed. Conclusions Using bioinformatic analysis of GEO combined with TCGA data, we revealed two independent prognostic indicators in LUAD and LUSC respectively and analyzed their clinical features. However, more detailed experiments and clinical trials are needed to verify their drug targets role in clinical medical use.
Development and psychometric validity of the Perioperative Anxiety Scale-7 (PAS-7)
Authors: Zhang Chengjiao , Liu Xitong , Hu Tianran , Zhang Fei , Pan Lingyi , Luo Yan , Wang Zhen 
Published: August 2020

Abstract
Background
Preoperative anxiety is a common psychological reaction in perioperative patients. The absence of a valid measurement tool of it hinders the evaluation of interventions to treat preoperative anxiety in China. This study aims to develop the Perioperative Anxiety Scale-7 (PAS-7) and to test its reliability, validity and cut-off value.
Methods
A total of 280 patients over 16 years old who were undergoing elective surgery were recruited to complete the PAS-7 and the Generalized Anxiety Disorder-7 scale (GAD-7) one day before surgery.
Results
The PAS-7 included 7 items, which were divided into two dimensions: mental anxiety and somatic anxiety. These two dimensions could explain 74.294% of the population variance. The internal consistency of each dimension ranged from 0.761–0.933. The confirmatory factor analysis showed that the model fit of the scale was good (χ2= 34.798, df = 13, χ2/df = 2.677, CFI = 0.949, GFI = 0.924, RMSEA = 0.115). The correlations between the GAD-7 and each dimension and the total score of the scale were significant. A cut-off score of 8, maximizing the Youden Index, yielded a sensitivity of 75% and a specificity of 84.6% (95% CI: 0.88–0.97).
Conclusions
The PAS-7 had good reliability and validity and could be used as an effective tool to evaluate preoperative anxiety.
Attenuated Self-serving Bias in People with Internet Gaming Disorder is Related to Altered Neural Activity in Subcortical-Cortical Midline Structures
Authors: Wang Yifan , Zheng Li , Wang Chenggong , Guo Xiuyan 
Published: August 2020

Abstract
Background
To protect and maintain the positivity of self-concept, normal people usually show a self-serving bias (internal attribution of positive events and external attribution of negative events) by the motives of self-enhancement and self-protection. And self-serving assessments predominantly activate the subcortical-cortical midline structures (CMS) in healthy individuals. However, little is known about the self-serving bias and its underlying neural correlates among people with Internet gaming disorder (IGD).
Methods
Twenty-four IGD and 25 recreational internet gaming users (RGU) were scanned while attributing causes of positive/negative self- and other-related events that could happen in both game world and real world contexts. Regions of interest (within CMS) analysis and parametric analysis were performed to investigate the neural correlates of self-serving bias in IGD.
Results
Behaviorally, the IGD participants attributed more negative and less positive events to themselves than RGU in both contexts. Neurally, during attributions of negative events, the IGD participants exhibited increased ventromedial prefrontal cortex (vmPFC) activation in both contexts compared to RGU. Higher vmPFC activation was associated with weaker self-protective motivation in IGD group. Meanwhile, during attributions of positive events, the IGD participants exhibited decreased precuneus/posterior cingulate cortex activation in real world compared to RGU. Parametric analysis showed reduced positive correlation between precuneus activation and self-attribution ratings of positive events in real world in IGD group relative to RGU group.
Conclusion
These results suggest that the IGD individuals show an attenuated self-serving bias and altered activations within CMS regions involved in self-attribution, providing evidence for the negative self-concept and weakened abilities in both self-enhancement and self-protection in IGD.
Gemini surfactant abetted synthesis of mesoporous Mn/Mg bimetal doped TiO2 nanomaterial: Characterization and photocatalytic activity studies under visible light irradiation
Authors: Miditana Sankara Rao , Tirukkovalluri Siva Rao , Raju Imandi Manga , Alim Shaik Abdul , Jaishree Genji , Chippada M.L.V. Prasanna 
Published: August 2020

Abstract
The present work mainly aimed to synthesize different weight percentages (0.25-1.00 wt%) of Manganese (Mn2+) and Magnesium (Mg2+) bimetal ions doped TiO2 nanomaterial assisted with different weight percentages (5-15 wt%) of Gemini Surfactant (GS) using sol-gel method. The bimetal doped and undoped TiO2 photocatalysts were characterized by X-ray Diffraction, Scanning Electron Microscopy, Energy Dispersive X-ray Spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), UV-Visible Diffused Reflectance Spectroscopy, Transmission Electron Microscopy, Brunauer-Emmett-Teller surface area analyzer, and Photoluminescence Spectroscopy. Characterization results evinced that Mn2+/Mg2+ bimetal ions doping and encapsulation of GS on TiO2 nanoparticles promote the formation of mesoporous multi-particle anatase TiO2 nanocatalysts with a narrowed band gap, less particle size, and high surface area. The surface elemental composition of the 0.25 wt% Mn2+& 1.00 wt% Mg2+ bimetal doped TiO2 in presence of 10 wt% of Gemini surfactant (after calcination) revealed the presence of both the metal dopants Mn2+ and Mg2+ along with the Ti and O and their chemical interactions were further confirmed by FT-IR results. The photocatalytic activity of these catalysts was assessed by the degradation of Methyl Red (MR) using visible light irradiation. To understand the effect of different reaction parameters on the photocatalytic activity of the nanocatalysts such as the dopant concentration, GS concentration, catalyst dosage, solution pH, and initial dye concentrations were investigated and optimized to achieve the best performance. The photoluminescence results conclude that OH radicals are the crucial reactive species responsible for oxidative photocatalytic degradation of MR.
Pathological α-synuclein triggers synaptic NMDA receptor dysfunction through altered trafficking
Authors: Huang Tingting , Gou De-Hai , Li Wen , Svanbergsson Alexander , Bouchet Delphine , Li Jia-Yi , Groc Laurent 
Published: August 2020

Abstract
Background

α-Synuclein misfolding and aggregation contribute to synaptic dysfunction in synucleinopathies, including Parkinson’s disease. However, the mechanism underlying the effect of α-synuclein on synaptic components remains unclear. Since the N-methyl-D-aspartic acid receptor (NMDAR) plays a key role in glutamate synapse pathophysiology, we here investigated its surface dynamics and functional distribution in neurons exposed to various pathological α-synuclein forms.
Methods

A combination of single-molecule tracking, immunochemistry, immunoblot and calcium imaging approaches were used to assess the changes in NMDAR membrane dynamics and functions. The NMDAR alterations were evaluated in rat cultured hippocampal networks, in which α-synuclein mutants were overexpressed or exposed to α-synuclein proteins (monomeric/PFF α-synuclein). The surface dynamics of NMDAR subtype was artificially tuned in order to test its instrumental role.
Results

We observed that mutant α-synuclein (A53T-α-synuclein) restricted NMDAR surface trafficking and impaired synaptic function. In contrast, wild-type α-synuclein did not affect synaptic NMDAR. Further, we found that chronic exposure to α-synuclein preformed fibrils induced molecular dysfunctions that mainly targeted the GluN2B-NMDAR subtype. The deficits of synaptic NMDAR have also been found in A53T transgenic mice α-synuclein. Upon fine-tuning of the surface dynamics of GluN2B-NMDAR, pathological α-synuclein gradually lost its synaptic toxicity.
Conclusions

Our findings indicate that pathological α-synuclein alters GluN2B-NMDAR synaptic dynamics and organization, which leads to glutamate synapse dysfunction.
Natural Malaria Infection in Anopheline Vectors and Their Incrimination in Local Malaria Transmission in Darién, Panama
Authors: Torres-Cosme Rolando , Rigg Chystrie , Santamaria Ana Maria , Vazquez Vanessa , Victoria Carlos , Ramirez Jose Luis , Calzada Jose E , Carrera Lorenzo Caceres 
Published: August 2020

Abstract
BackgroundMore than 85% of the malaria cases in Panama occur in characteristically poor, rural and indigenous regions like Darien Province. Vector diversity, vector infection rate and spatial distribution are important entomological parameters of malaria transmission dynamics. Their understanding is crucial for the development of effective disease control strategies. The objective of this study was to determine the composition of Anopheles species, their natural infection rate and their geographic distribution to better understand the malaria transmission dynamics in Darién, Panama.MethodsAnopheline mosquitoes were captured during the rainy and dry season of 2016. We selected five communities where adult anophelines were collected using CDC light-traps, and through protective human-baited traps. Detection of natural infection and Plasmodium genotype in collected mosquitoes were detected via nested PCR through the amplification of Plasmodium 18s rRNA and the circumsporozoite protein gene, respectively.ResultsA total of 1,063 mosquitoes were collected, and nine Anopheline species were identified, with the predominant species being: An. (Nys.) darlingi (45.0%) and An. (Nys.) albimanus (42.6%). Among these mosquitoes, An. (Nys.) albimanus has historically presented an elevated frequency and abundance in all Panamanian regions. Natural infection with P. vivax was detected in a mosquito pool from the community Pueblo Tortuga (0.6%), three mosquito pools from Marraganti (1.7%), two pools from Bajo Chiquito (1.1%) and three pools from Alto Playona (1.7%). For An. (Nys.) darlingi mosquitoes, we detected seven positive pools from the community Bajo Chiquito (4.0%), two pools from Marraganti (1.1%) and two pools from Alto Playona (1.1%). This study was able to detect the P. vivax allelic variant VK210 in infected mosquitoes.ConclusionThe results from this study provide new information on the transmission dynamics associated with anopheline vectors in the Darién region. This is the first report of natural P. vivax infection in An. (Nys.) darlingi and its incrimination as a potential malaria vector in Panama. Additional studies are necessary to expand our knowledge and determine crucial parameters in malaria transmission in Darién, which in turn will aid the National Malaria Program in attaining an adequate malaria control strategy towards malaria elimination.
GRAPES-DD: Exploiting Decision Diagrams for Index-Driven Biomedical Databases Search
Authors: Licheri Nicola , Bonnici Vincenzo , Beccuti Marco , Giugno Rosalba 
Published: August 2020

Abstract
Background: Graphs are mathematical structures widely used for expressing relationships among elements when representing biomedical and biological information. On top of these representations, several analyses are conducted. A common task is the search of one substructure, called query, within one graph, called target. The problem is referred to as one-to-one subgraph search, and it is shown to be NP-complete. However, heuristics and indexing techniques can be applied to facilitate the search. Such indexing techniques are also exploited in the context of searching in a collection of target graphs, referred to as one-to-many subgraph problem. Filter-and-veriﬁcation methods that use indexing approaches provide a fast pruning of target graphs or parts of them that do not contain the query. The expensive veriﬁcation phase is then performed only on the subset of promising targets. Indexing strategies extract graph features at a suﬃcient granularity level for performing a powerful ﬁltering step. Features are memorized in data structures allowing an eﬃcient access. Indexing size, querying time and ﬁltering power are key points for the development of eﬃcient subgraph searching solutions. Results: An existing subgraph approach, GRAPES, has been shown to have good performance in term of speed-up for both one-to-one and one-to-many cases. However, it suﬀers in the size of the built index. For this reason, we propose GRAPES-DD, a modiﬁed version of GRAPES in which the indexing structure has been replaced with a Decision Diagram. Decision Diagrams are a broad class of data structures widely used to encode and manipulate functions eﬃciently. Our experiments on real biomedical structures and synthetic graphs have conﬁrmed our expectation showing that GRAPES-DD has substantially reduced the memory utilization with respect to GRAPES without worsening the searching time. Conclusion: The use of Decision Diagrams for searching in biomedical graphs is completely new and potentially promising thanks to their ability to encode compactly sets by exploiting their structure and regularity, and (ii) to manipulate entire sets of elements at once, instead of exploring each single element explicitly. This work shows that search strategies based on Decision Diagram makes the indexing for biochemical graphs, and not only, more scalable and aﬀordable allowing us to potentially deal with huge and ever growing collections of biomedical structures.
NPM1 mutation mediated HOXBLINC lncRNA activation promotes AML leukemogenesis
Authors: Zhu Ganqian , Luo Huacheng , Feng Yang , Guryanova Olga , Xu Jianfeng , Chen Shi , Lai Qian , Xu Bing , Zhao Zhigang , Ni Hongyu , Claxton David , Guo Ying , Qiu Yi , Yang Feng-Chun , Li Wei , Nimer Stephen , Huang Suming , Xu Mingjiang 
Published: August 2020

Abstract
Nucleophosmin (NPM1) is the most commonly mutated gene in acute myeloid leukemia (AML) resulting in aberrant cytoplasmic translocation of the encoded nucleolar protein (NPM1c+). NPM1c+ maintains a unique leukemic gene expression program, characterized by activation of HOXA/B clusters and MEIS1 oncogene to facilitate leukemogenesis. However, the mechanisms by which NPM1c+ controls such gene expression patterns to promote leukemogenesis remain largely unknown. Here, we show that the activation of HOXBLINC, a HOXB locus-associated long non-coding RNA (lncRNA), is a critical downstream mediator of NPM1c+-associated leukemic transcription program and leukemogenesis. HOXBLINC loss attenuates NPM1c+-driven leukemogenesis by rectifying the signature of NPM1c+ leukemic transcription programs. Furthermore, overexpression of HoxBlinc (HoxBlincTg) in mice enhances HSC self-renewal and expands myelopoiesis, leading to the development of AML-like disease, reminiscent of the phenotypes seen in the Npm1 mutant knock-in (Npm1c/+) mice. HoxBlincTg and Npm1c/+ HSPCs share significantly overlapped transcriptome and chromatin structure. Mechanistically, HoxBlinc binds to the promoter regions of NPM1c+ signature genes to control their activation in HoxBlincTg HSPCs, via MLL1 recruitment and promoter H3K4me3 modification. Our study reveals that HOXBLINC lncRNA activation plays an essential oncogenic role in NPM1c+ leukemia. HOXBLINC and its partner MLL1 are potential therapeutic targets for NPM1c+ AML.
Predictors of Favorable Outcomes for Vertebrobasilar Artery Occlusion after Endovascular Therapy within 24 Hours of Symptom Onset
Authors: Jiang Changchun , Fan Yu , Li Yuechun , Hao Fei , Yang Junfeng , Wang Baojun , Zhang Tianyou , Li Xia , Liu Jiahui , Wang Shuguang 
Published: August 2020

Abstract
Objective
The aim of the present study was to describe our results of endovascular therapy (EVT) for vertebrobasilar artery occlusion (VBAO) within 24 h of symptom onset, and to evaluate prognostic factors associated with favorable outcomes.
Methods
The present study enrolled patients who underwent EVT for acute ischemic stroke (AIS) caused by VBAO. Inclusion criteria for EVT to treat VBAO were as follows: (1) computed tomography angiography (CTA) or magnetic resonance angiography (MRA) confirmed acute VBAO; (2) baseline National Institutes of Health Stroke Scale (NIHSS) score ≥ 2; (3) premorbid modified Rankin scale (mRS) score ≤ 2; (4) onset or last known time to puncture within 24 h; and (5) posterior-circulation Acute Stroke Prognosis Early CT score (pc-ASPECTS) ≥ 6. Favorable outcomes were defined as mRS scores of 0–3 at three months following EVT. The associations among baseline parameters, procedural parameters, and favorable outcomes were assessed.
Results
A total of 67 patients were recruited in this study, of which 40 patients (59.7%) had favorable outcomes. Of the 50 patients with a late-window (6–24 h), 29 patients (58%) had favorable outcomes. Univariate analysis revealed significant associations of the following parameters with favorable outcomes in the enrolled patients: age, sex, smoking status, baseline NIHSS score, baseline Glasgow coma scale (GCS) score, Pons-midbrain index (PMI), and intracranial atherosclerosis (ICAS). Multivariate logistic regression indicated that only age (OR 0.914, 95% CI: 0.849 to 0.984; p = 0.017), baseline GCS score (OR 1.234, 95% CI: 1.061 to 1.435; p = 0.006), and PMI (OR 0.448, 95% CI: 0.252 to 0.798; p = 0.006) were independently correlated with favorable outcomes at three months following EVT. After adjustments for confounding factors in patients with a late-window, only age (OR 0.879, 95% CI: 0.799 to 0.967; p = 0.008) was associated with favorable outcomes.
Conclusions
Younger age, lower PMI, and higher GCS scores in patients with VBAO-induced AIS were associated with more favorable outcomes. In late-window (6–24 h) patients, younger age correlated to favorable outcomes, and lower NIHSS scores and lower PMI each also had a tendency to correlate with favorable outcomes.