bluuebunny/crossref_metadata_2025_split huggingface dataset:
No Evidence for Long-range Male Sex Pheromones in Two Malaria Mosquitoes
Authors: Poda Serge Bèwadéyir , Buatois Bruno , Lapeyre Benoit , Dormont Laurent , Diabaté Abdoulaye , Gnankiné Olivier , Dabiré Roch K. , Roux Olivier 
Published: July 2020

AbstractCues involved in mate seeking and recognition prevent hybridization and can be involved in speciation processes. In malaria mosquitoes, females of the two sibling speciesAnopheles gambiae s.s.andAn. coluzziimate in monospecific male swarms and hybrids are rare. Long-range sex pheromones driving this behavior have been debated in literature but to date, no study has proven their existence or their absence. Here, we attempted to bring to light their existence. To put all the odds in our favor, we used different chemical ecology methods such as behavioral and electrophysiological assays as well chemical analyses, and we worked with mosquitoes at their optimal physiological mating statei.e.with swarming males during their natural swarming windows. Despite all our efforts, our results support the absence of long-range sex pheromones involved in swarm detection and recognition by females. We briefly discuss the implications of this finding in ecology, evolution and for control strategies.
Comparison of manual and machine learning image processing approaches to determine fungiform papillae on the tongue
Authors: Cattaneo Camilla , Liu Jing , Wang Chenhao , Pagliarini Ella , Sporring Jon , Bredie Wender L.P. 
Published: July 2020

AbstractHuman taste perception is associated with the papillae on the tongue as they contain a large proportion of chemoreceptors for basic tastes and other chemosensation. Especially the density of fungiform papillae (FP) is considered as an index for responsiveness to oral chemosensory stimuli. The standard procedure for FP counting involves visual identification and manual counting of specific parts of the tongue by trained operators. This is a tedious task and automated image analysis methods are desirable. In this paper a machine learning image processing method based on a convolutional neural network is presented. This automated method was compared with three standard manual FP counting procedures using tongue pictures from 132 subjects. Automated FP counts, within the selected areas and the whole tongue, significantly correlated with the manual counting methods (all ρs ≥ 0.76). When comparing the images for gender and PROP status, the density of FP predicted from automated analysis was in good agreement with data from the manual counting methods, especially in the case of gender. Moreover, the present results reinforce the idea that caution should be applied in considering the relationship between FP density and PROP responsiveness since this relationship can be an oversimplification of the complexity of phenomena arising at the central and peripherical levels. Indeed, no significant correlations were found between FP and PROP bitterness ratings using the automated method for selected areas or the whole tongue. Besides providing estimates of the number of FP, the machine learning approach used a tongue coordinate system that normalizes the size and shape of an individual tongue and generated a heat map of the FP position and normalized area they cover. The present study demonstrated that the machine learning approach could provide similar estimates of FP on the tongue as compared to manual counting methods and provide estimates of more difficult-to-measure parameters, such as the papillae’s areas and shape.
The Impact of Gag Non-Cleavage Site Mutations on HIV-1 Viral Fitness from Integrative Modelling and Simulations
Authors: Samsudin Firdaus , Gan Samuel Ken-En , Bond Peter J. 
Published: July 2020

SummaryThe high mutation rate in retroviruses is one of the leading causes of drug resistance. In human immunodeficiency virus type-1 (HIV-1), synergistic mutations in its protease and the protease substrate – the Group-specific antigen (Gag) polyprotein – work together to confer drug resistance against protease inhibitors and compensate the mutations affecting viral fitness. Some Gag mutations can restore Gag-protease binding, yet most Gag-protease correlated mutations occur outside of the Gag cleavage site. To investigate the molecular basis for this, we now report multiscale modelling approaches to investigate various sequentially cleaved Gag products in the context of clinically relevant mutations that occur outside of the cleavage sites, including simulations of the largest Gag proteolytic product in its viral membrane-bound state. We found that some mutations, such as G123E and H219Q, involve direct interaction with cleavage site residues to influence their local environment, while certain mutations in the matrix domain lead to the enrichment of lipids important for Gag targeting and assembly. Collectively, our results reveal why non-cleavage site mutations have far-reaching implications outside of Gag proteolysis, with important consequences for drugging Gag maturation intermediates and tackling protease inhibitor resistance.
The ER membrane chaperone Shr3 acts in a progressive manner to assist the folding of related plasma membrane transport proteins
Authors: Myronidi Ioanna , Ring Andreas , Ljungdahl Per O. 
Published: July 2020

AbstractProteins with multiple membrane-spanning segments (MS) co-translationally insert into the endoplasmic reticulum (ER) membrane of eukaryotic cells. InSaccharomyces cerevisiae, Shr3 is an ER membrane-localized chaperone that is specifically required for the functional expression of amino acid permeases (AAP), a family of eighteen transporters comprised of 12 MS. Here, comprehensive scanning mutagenesis and deletion analysis of Shr3, combined with a modified split-ubiquitin approach, were used to probe chaperone-substrate interactions with seven different AAPin vivo. Our findings indicate that Shr3 specifically recognizes AAP substrates, largely independent of sequence-specific interactions involving membrane and luminally oriented domains. Shr3 selectively and robustly interacts with nested C-terminal AAP truncations in marked contrast to similar truncations of non-Shr3 substrate polytopic sugar transporters. Strikingly, Shr3-AAP interactions initiate with the first 4 MS of AAP and successively strengthen, but abruptly weaken when all 12 MS are present. The data are consistent with Shr3 acting in a temporal manner as a scaffold preventing AAP translation intermediates from engaging in non-productive interactions.
STAMP: a multiplex sequencing method for simultaneous evaluation of mitochondrial DNA heteroplasmies and content
Authors: Guo Xiaoxian , Wang Yiqin , Zhang Ruoyu , Gu Zhenglong 
Published: July 2020

ABSTRACTHuman mitochondrial genome (mtDNA) variations, such as mtDNA heteroplasmies (the co-existence of mutated and wild-type mtDNA), have received increasing attention in recent years for their clinical relevance to numerous diseases. But large-scale population studies of mtDNA heteroplasmies have been lagging due to the lack of a labor- and cost-effective method. Here, we present a novel human mtDNA sequencing method called STAMP (sequencing bytargetedamplification ofmultiplexprobes) for measuring mtDNA heteroplasmies and content in a streamlined workflow. We show that STAMP has high mapping rates to mtDNA, deep coverage of unique reads, and high tolerance to sequencing and PCR errors when applied to human samples. STAMP also has high sensitivity and low false positive rates in identifying artificial mtDNA variants at fractions as low as 0.5% in genomic DNA samples. We further extend STAMP, by including nuclear DNA-targeting probes, to enable assessment of relative mtDNA content in the same assay. The high cost-effectiveness of STAMP, along with the flexibility of using it for measuring various aspects of mtDNA variations, will accelerate the research of mtDNA heteroplasmies and content in large population cohorts, and in the context of human diseases and aging.
Novel algorithms for PFGE bacterial typing: Number of co-migrated DNA fragments, linking PFGE to WGS results and computer simulations for evaluation of PulseNet international typing protocols
Authors: Adam Ibrahim-Elkhalil M. , Abdokashif Isam , Elrashid Asia , Bayoumi Hiba , Musa Ahmed , Abdulgyom Eithar , Mamoun Safaa , Alnagar Sittana , Mohammed Wafaa , El-khateeb Amna , Oshi Musaab , El-bakri Faris 
Published: July 2020

AbstractBackgroundStandard protocols for Pulsed-field gel electrophoresis (PFGE) were adopted and being used in a global scale for surveillance of many bacterial food-borne diseases. Matched PFGE bands are considered regardless of co-migration of different DNA fragments. Molecular epidemiology is turning toward whole genome sequencing (WGS). Although, WGS results can be digestedIn-silico, PFGE and WGS data are being compared separately. We describe a new image analysis algorithm that enables identification of how many DNA fragments co-migrate during PFGE. We built a database that compare described PFGE results toin-silicoobtained digestion models (from WGS). Reliability of the method was assessedin-silicousing novel computer simulation approach. From WGS, 1,816 digestion model (DMs) were obtained as recommended by PulseNet international. Simulation codes were designed to predict PFGE profiles when DMs are separated at 5% PFGE resolution in addition to expected co-migration levels.ResultsPFGE simulation has shown that about 35% of DNA fragments co-migrate at 5% PFGE resolution. Similar result was obtained when wet-lab PFGE profiles were analyzed using image analysis algorithm mentioned earlier. When image analysis results were compared to DMs, results returned bygeltowgs.uofk.edudatabase revealed reasonable relatedness to DMs. In terms of number of PFGE typable DNA fragments, 45,517 were typable (representing 46.54% out of 97,801). Previously mentioned typable fragments (in terms of typable sizes) comprised 91.24% of the sum of nucleotides of all chromosomes tested (7.24 billion bp). However, significant variations were shown within and between different digestion protocols.ConclusionsIdentification of co-migration levels will reveal the third dimension of PFGE profiles. This will provide a better way for evaluating isolate relationships. Linking old PFGE results to WGS by means of simulation demonstrated here will provide a chance to link millions of PFGE epidemiological data accumulated during the last 24 years to the new WGS era. Evaluation of population dynamics of pathogenic bacteria will be deeper through space and time. Selection of restriction enzymes for PFGE typing will have a powerfulin-silicoevaluation tool.
Impact of climate on COVID-19 transmission: A case study with Indian states
Authors: Manik Souvik , Mandal Manoj , Pal Sabyasachi , Patra Subhradeep , Acharya Suman 
Published: July 2020

AbstractCoronavirus Disease 2019 (COVID-19) started in Wuhan province of China in November 2019 and within a short time, it was declared as a worldwide pandemic by the World Health Organisation due to the very fast worldwide spread of the virus. There were a few studies that look for the correlation with infected individuals and different environmental parameters using early data of COVID-19 but there was no study that deal with the variation of effective reproduction number and environmental factors. Effective reproduction number is the driving parameter of the spread of a pandemic and it is important to study the effect of various environmental factors on effective reproduction numbers to understand the effect of those factors on the spread of the virus. We have used time-dependent models to investigate the variation of different time-dependent driving parameters of COVID-19 like effective reproduction number and contact rate using data from India as a test case. India is a large population country that was highly affected due to the COVID-19 pandemic and has a wide span of different temperature and humidity regions and is ideal for such study. We have studied the impact of temperature and humidity on the spread of the virus of different Indian states using time-dependent epidemiological models SIRD, and SEIRD for a long time scale. We used a linear regression method to look for any dependency between the effective reproduction number with the relative humidity, absolute humidity, and temperature. The effective reproduction number showed a negative correlation with both relative and absolute humidity for most of the Indian states, which are statistically significant. This implies that relative and absolute humidity may have an important role in the variation of effective reproduction numbers. There was no conclusive evidence of a correlation between effective reproduction numbers and average air temperature.
Attenuated Anticipation of Social and Monetary Rewards in Autism Spectrum Disorders
Authors: Baumeister Sarah , Moessnang Carolin , Bast Nico , Hohmann Sarah , Tillmann Julian , Goyard David , Charman Tony , Ambrosino Sara , Baron-Cohen Simon , Beckmann Christian , Bölte Sven , Bourgeron Thomas , Rausch Annika , Crawley Daisy , Dell’Acqua Flavio , Dumas Guillaume , Durston Sarah , Ecker Christine , Floris Dorothea L. , Frouin Vincent , Hayward Hannah , Holt Rosemary , Johnson Mark H. , Jones Emily J.H. , Lai Meng-Chuan , Lombardo Michael V. , Mason Luke , Oldehinkel Marianne , Persico Tony , Cáceres Antonia San José , Wolfers Thomas , Spooren Will , Loth Eva , Murphy Declan G. M. , Buitelaar Jan K. , Tost Heike , Meyer-Lindenberg Andreas , Banaschewski Tobias , Brandeis Daniel ,   the AIMS-2-TRIALS group
Published: July 2020

AbstractBackgroundReward processing has been proposed to underpin atypical social behavior, a core feature of autism spectrum disorder (ASD). However, previous neuroimaging studies have yielded inconsistent results regarding the specificity of atypicalities for social rewards in ASD. Utilizing a large sample, we aimed to assess altered reward processing in response to reward type (social, monetary) and reward phase (anticipation, delivery) in ASD.MethodsFunctional magnetic resonance imaging during social and monetary reward anticipation and delivery was performed in 212 individuals with ASD (7.6-30.5 years) and 181 typically developing (TD) participants (7.6-30.8 years).ResultsAcross social and monetary reward anticipation, whole-brain analyses (p<0.05, family-wise error-corrected) showed hypoactivation of the right ventral striatum (VS) in ASD. Further, region of interest (ROI) analysis across both reward types yielded hypoactivation in ASD in both the left and right VS. Across delivery of social and monetary reward, hyperactivation of the VS in individuals with ASD did not survive correction for multiple comparisons. Reward type by diagnostic group interactions, and a dimensional analysis of autism trait scores were not significant during anticipation or delivery. Levels of attention-deficit/hyperactivity disorder (ADHD) symptoms did not affect reward processing in ASD.ConclusionsOur results do not support current theories linking atypical social interaction in ASD to specific alterations in processing of social rewards. Instead, they point towards a generalized hypoactivity of VS in ASD during anticipation of both social and monetary rewards. We suggest that this indicates attenuated subjective reward value in ASD independent of social content and ADHD symptoms.
A failure case of P110 steel tubing in CO2 flooding well
Authors: Song Longfei , Liu Zhiyong , Lu Lin , Li Xiaogang , Sun BaoZhuang , Cheng Huanlin 
Published: July 2020

Purpose
This paper aims to analyze a failure case of a P110 tube in a CO2 flooding well.

Design/methodology/approach
The chemical composition, microstructure and mechanical properties of the failed P110 tubing steel were tested, and met the API Spec 5CT standard. The fractures were investigated by scanning electron microscopy and energy dispersive spectroscopy.

Findings
Fracture was induced by stress corrosion cracking (SCC) and that the stress concentration caused by the mechanical damage played an important role in the failure. The failure case is a SCC failure affected by mechanical damage and galvanic corrosion.

Originality/value
The effect of the infiltration of groundwater was studied in the failure case. The stress concentration caused by the mechanical damage played an important role in the failure.
AHP versus DEA: a comparative analysis for the gradual improvement of unsustainable suppliers
Authors: Rashidi Kamran 
Published: July 2020

PurposeData envelopment analysis (DEA) and analytical hierarchy process (AHP) are two widely applied methods to evaluate and rank suppliers in terms of sustainability. In this study, to investigate the extent to which potential differences in the outcomes of these two methods influence the benchmarking strategies, a comparative analysis based on a common set of data gathered from 19 logistics service providers is implemented.Design/methodology/approachAs suppliers' sustainability cannot be improved in a single-step process due to several limitations, improvement needs to proceed gradually. Therefore, using the self-organising map method, the suppliers were classified into clusters within a novel framework for gradually improving their sustainability. Then, the two processes of gradual improvement based on the outcomes of DEA and AHP were compared.FindingsThe findings show that although the rankings of suppliers guided by the methods correlated to a high degree, the benchmarking strategies provided by the methods for gradually improving the sustainability of suppliers differed considerably. In particular, whereas AHP suggests a benchmarking policy better suited for unsustainable or less sustainable suppliers with limited access to resources, DEA proposes one for suppliers able to dramatically boost their sustainability with few quick, significant leaps in performance.Originality/valueFirst, this study revealed a novel gradual improvement framework using the self-organising map method. Second, it clarified the extent to which the benchmarking policies are influenced by the type of evaluation method.
Tackling the HR digitalization challenge: key factors and barriers to HR analytics adoption
Authors: Fernandez Vicenc , Gallardo-Gallardo Eva 
Published: July 2020

Purpose
This paper aims to contribute to the literature on human resources (HR) digitalization, specifically on HR analytics, disentangling the concept of analytics applied to HR and explaining the factors that hinder companies from moving to analytics. Therefore, the central research questions addressed in this study are: what does HR analytics encompass? What impedes the adoption of analytics in HR within organizations?

Design/methodology/approach
The authors performed a comprehensive literature review on analytics as applied in HR. The authors relied on two of the major multidisciplinary publication databases (i.e. Scopus and WoS). A total of 64 manuscripts from 2010 to 2019 were content analyzed.

Findings
The results reveal that there is an ongoing confusion on HR analytics conceptualization. Yet, it seems that there is an emerging consensus on what HR analytics encompasses. The authors have identified 14 different barriers for HR analytics adoption grouped into four categories, namely, data and models, software and technology, people and management. Grounding on them the authors propose a set of 14 key factors to help to successfully adopt HR Analytics in companies.

Originality/value
This paper brings clarity over the conceptualization of HR analytics by offering a comprehensive definition. Additionally, it facilitates business and HR leaders in making informed decisions on adopting and implementing HR analytics. Moreover, it assists HR researchers in positioning their paper more explicitly in current debates and encouraging them to develop some future avenues of research departing from some questions posed.
Analysis of sponsorship networks and cross-domain knowledge exchange: an empirical study on Zhihu
Authors: Qi Tuotuo , Wang Tianmei , Chen Nuo 
Published: July 2020

PurposeThis study aims to analyze the structural characteristics of knowledge exchange in Zhihu Lives to provide feasible suggestions for improving the creative enthusiasm of knowledge providers.Design/methodology/approachThis paper uses the domain classification of Zhihu Lives to construct a cross-domain knowledge exchange network.FindingsThis research makes the following findings: the small-world effect exists in the sponsorship network and is conducive to enhancing the learning willingness of knowledge providers; significant sponsorships and strong learning willingness exist among knowledge providers; the knowledge exchange is obvious among the fields of education, reading and writing, business and lifestyle and the fields of music, film, games, art, the internet, science and technology, design, financial economy and occupation; and knowledge exchange is obvious among the internal fields of education, reading and writing, and business and life style, between the internal fields of music, film, and games and art and between the internal fields of the internet, science and technology, design, financial economy and occupation.Originality/valueThis study can provide practical suggestions for the following development of the platform by analyzing the special phenomenon of knowledge exchange in the present stage of knowledge exchange.
Gratitude, social intelligence, and leadership among university teachers: mediation and moderation analysis
Authors: Garg Naval , Jain Anuradha , Punia B.K. 
Published: July 2020

Purpose
The purpose of this study is to investigate the linkage between gratitude and leadership among university teachers. It also tended to examine mediating and moderating effect of social intelligence amidst the relationship between gratitude and leadership. As social intelligence is a multi-dimensional concept, this paper explored mediating and moderating effect of three dimensions of social intelligence, i.e. social awareness, social skills and social information processing.

Design/methodology/approach
Mediation effect was examined with help of Baron and Kenny’s (1986) technique, Sobel test and bootstrapping estimates. Significance of interaction between independent variable (gratitude) and moderator (social intelligence) was investigated for moderation analysis.

Findings
The findings reported partial mediating effect of all three dimensions of social intelligence. No significant moderation effect was observed.

Originality/value
This is one of the pioneer studies that explored interrelationship between gratitude, social intelligence and leadership among Indian University teachers.