bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole-genome bisulfite sequencing of abdominal adipose reveals DNA methylation pattern variations in broiler lines divergently selected for fatness
Authors: Gong Pengfei , Jing Yang , Liu Yumeng , Wang Lijian , Wu Chunyan , Du Zhiqiang , Li Hui 
Published: December 2020

AbstractThe methylation status of pivotal genes involved in fat deposition in chickens has been extensively studied. However, the whole-genome DNA methylation profiles of broiler abdominal adipose tissue remain poorly understood. Using whole-genome bisulfite sequencing, we generated DNA methylation profiles of chicken abdominal adipose tissue from Northeast Agricultural University broiler lines divergently selected for abdominal fat content. We aimed to explore whether DNA methylation was associated with abdominal fat deposition in broilers. The whole-genome DNA methylation profiles of fat- and lean-line broilers abdominal adipose tissue were constructed. The DNA methylation levels of functional genomic regions in the fat broiler were higher than those in the lean broiler, especially in the 3′ untranslated regions (UTRs) and exons in the non-CG contexts. Additionally, we identified 29,631 differentially methylated regions and, subsequently, annotated 6,484 and 2,016 differentially methylated genes (DMGs) in the gene body and promoter regions between the two lines, respectively. Functional annotation showed that the DMGs in promoter regions were significantly enriched mainly in the triglyceride catabolic process, lipid metabolism-related pathways, and extracellular matrix signal pathways. When the DMG in promoter regions and differentially expressed genes were integrated, we identified 30 genes with DNA methylation levels that negatively correlated with their messenger RNA (mRNA) expression, of which CMSS1 reached significant levels (false discovery rate &lt; 0.05). These 30 genes were mainly involved in fatty acid metabolism, peroxisome-proliferator-activated receptor signaling, Wnt signaling pathways, transmembrane transport, RNA degradation, and glycosaminoglycan degradation. Comparing the DNA methylation profiles between fat- and lean-line broilers demonstrated that DNA methylation is involved in regulating broiler abdominal fat deposition. Our study offers a basis for further exploring the underlying mechanisms of abdominal adipose deposition in broilers.
Are Yeast Autolysate Attractants for Ceratitis Species (Diptera: Tephritidae) in South Africa More Attractive and Palatable Than a Currently Used Protein Attractant?
Authors: Grout Timothy G , Stephen Peter R 
Published: December 2020

Abstract
Due to increasing usage of copper fungicides in citrus orchards shortly before the application of protein baits for fruit fly control and the risk of this combination causing fruit phytotoxicity, an investigation of attractants containing higher amounts of yeast autolysate rather than protein hydrolysate was conducted. A commercial yeast autolysate from Australia was less attractive than a South African product HymLure (with a mixture of autolysate and hydrolysate) to three Ceratitis species: Ceratitis capitata (Wiedemann), Ceratitis rosa s.s. Karsch, and Ceratitis cosyra (Walker). Other custom-formulated autolysates were also inferior to HymLure for these species. Consumption of autolysates during the first hour of feeding was similar to that of HymLure. The inferior attraction of these three species of fruit flies to autolysates, relative to one containing hydrolysate, did not warrant further investigation of autolysates for use in baits to control these quarantine pests.
First come, first served: fruit availability to keystone bat species is potentially reduced by invasive macaques
Authors: Reinegger Raphael D , Oleksy Ryszard Z , Bissessur Prishnee , Naujeer Houshna , Jones Gareth 
Published: December 2020

AbstractBats provide important pollination and seed-dispersal services to native angiosperms. However, many bat species are increasingly threatened by human disturbance, including the Mauritian flying fox (Pteropus niger), an endemic, keystone seed disperser. Native forests are scarce and P. niger frequently feeds in commercial plantations, where it now is considered a pest and subjected to frequent culling, thereby hindering conservation efforts. The invasive long-tailed macaque (Primates: Cercopithecidae, Macaca fascicularis) potentially competes with P. niger for scarce native fruits. We investigated the extent of dietary overlap between M. fascicularis and P. niger on Mauritius by sampling fruit drop for 17 tree species and identifying additional food species along line transects. Fruits of 13 of 17 species were eaten by animals and fruit production across tree replicates generally was low but highly variable. Although M. fascicularis ate only 4% of fruit overall, they consumed 20–100% of the fruits of seven species. Approximately 39% of dropped fruits were intact; based on field observations, most probably were dropped by M. fascicularis. Unlike P. niger, M. fascicularis ate mostly unripe fruit and depleted all fruit of certain species at an unripe stage. Hence, M. fascicularis may restrict P. niger’s diet and potentially disrupt seed dispersal of some tree species. Furthermore, small trees are more prone to fruit depletion at an unripe stage by macaques. In addition, asynchronous fruiting phenology across forest fragments may modulate the provision of native fruits to P. niger throughout the year. Although competition can be demonstrated only by controlled experimental studies that are logistically impossible in our scenario, our results highlight potential detrimental consequences that introduced frugivores may have on keystone seed dispersers. Finally, our results suggest that a more integrative and island-wide approach to forest restoration may be valuable for the conservation of P. niger.
Child Marriage in Yemen: A Mixed Methods Study in Ongoing Conflict and Displacement
Authors: Hunersen Kara , Attal Bothaina , Jeffery Allison , Metzler Janna , Alkibsi Tareq , Elnakib Shatha , Robinson W Courtland 
Published: December 2020

Abstract
Objectives: This study assesses the prevalence of and risk factors for child marriage in Yemen, which was experiencing a nationwide conflict at the time of the study. Study Design: We conducted a survey of internally displaced persons (IDPs) and host communities using a stratified multistage cluster sampling design. Each household included an interview with a female adult (n=1210), a household roster (n=8400), and one female adolescent interview (n=1210). We used multivariate logistic regression to assess the association between child marriage and various risk factors. We also used data from focus groups (n=411) and key informant interviews (n=30) to explore community perspectives and understand contextual factors relating to child marriage. Results: Prevalence of child marriage among IDP females aged 10-19 was 18.1% compared to 12.7% among hosts. In the regression model, being older (aOR=1.95), never attending school (aOR=3.94), place of origin of Saada (aOR=4.41), and unemployment of the female adult (aOR=2.84) showed increased odds of child marriage. Head of household unemployment (aOR=0.58) and completed higher education (aOR=0.42) showed decreased odds. Qualitatively, economic factors were cited as central factors in decision-making, both for host communities and even more so for IDPs. Perceptions of marriage readiness and negative consequences of child marriage were dependent on gender normative expectations. Conclusions: Displaced girls experience child marriage more than boys or host girls . Displacement effects economic security and household power dynamics, which affects marriage decision-making and girl s ability to self-advocate. Efforts to address child marriage in Yemen should include livelihood support, with awareness and conflict management components that start in pre-adolescence and include married and unmarried girls.
Challenges in Oncologic Preparedness: A Retrospective Review of Incident Surgical Cancers During Pacific Partnership Missions, 2008-2016
Authors: Reichl Allison , Schutt Ryan , Walsh John , Prieto James , Sykes Alicia G , Bickler Stephen , Ignacio Romeo 
Published: December 2020

ABSTRACT

Introduction
For over 30 years, the USNS Mercy hospital ship has provided surgical care on multiple humanitarian aid and disaster relief missions. During these missions, surgical support varies according to host nation needs, and the operative treatment of cancer patients remains controversial. We report the number of incidentally discovered surgical oncologic cases treated aboard the USNS Mercy on four missions and discuss challenges regarding oncologic care on these missions.

Materials and Methods
Between 2008 and 2016, operative cases and surgical pathology results from four multinational humanitarian missions were analyzed according to organ system, patient’s geographic location, and diagnosis. Primary outcomes were total number and proportion of malignant cases, analyzed yearly and over all four missions. Secondary outcomes were malignant diagnoses by organ system and host nation health capacities (based on indicators from the WHO).

Results
A total of 2,767 operations were performed during 18 port visits in 8 countries in Southeast Asia. In total, 1,193 pathology specimens (surgical biopsies, fine needle aspirations, etc.) were obtained. Overall malignancy rate across all organ systems was 9%. Yearly malignancy rates ranged from 2% to 13%. The highest malignancy rates were found in thyroid (33%), breast (20%), and parotid and salivary gland cases (19%). All host nations had operational strategies for cancer in place (n = 8, 100%), but few had national infrastructures to treat noncommunicable diseases (n = 2, 25%).

Conclusions
Despite current policies to screen out cancer patients on USNS Mercy missions, 9% of surgical biopsies were malignant. Cancer management during these missions presents a unique challenge because of limited resources for surgery, chemoradiotherapy, and follow-up care. Contingency plans must be considered to provide completion of care for these patients whose cancers are discovered incidentally. Furthermore, an understanding of host nation capabilities in relation to medical and surgical care is crucial to providing treatment in resource-limited areas.
The Glossopharyngo-Cochlear Triangle—Part II: Case Series Highlighting the Clinical Application to High-Riding Posterior Inferior Cerebellar Artery Aneurysms Exposed Through the Extended Retrosigmoid Approach
Authors: Baranoski Jacob F , Koester Stefan W , Przybylowski Colin J , Zhao Xiaochun , Catapano Joshua S , Gandhi Sirin , Tayebi Meybodi Ali , Cole Tyler S , Lee Jonathan , Frisoli Fabio A , Lawton Michael T , Mascitelli Justin R 
Published: December 2020

Abstract

BACKGROUND
Use of the far lateral transcondylar (FL) approach and vagoaccessory triangle is the standard exposure for clipping most posterior inferior cerebellar artery (PICA) aneurysms. However, a distal PICA origin or high-lying vertebrobasilar junction can position the aneurysm beyond the vagoaccessory triangle, making the conventional FL approach inappropriate.

OBJECTIVE
To demonstrate the utility of the extended retrosigmoid (eRS) approach and a lateral trajectory through the glossopharyngo-cochlear triangle as the surgical corridor for these cases.

METHODS
High-riding PICA aneurysms treated by microsurgery were retrospectively reviewed, comparing exposure through the eRS and FL approaches. Clinical, surgical, and outcome measures were evaluated. Distances from the aneurysm neck to the internal auditory canal (IAC), jugular foramen, and foramen magnum were measured.

RESULTS
Six patients with PICA aneurysms underwent clipping using the eRS approach; 5 had high-riding PICA aneurysms based on measurements from preoperative computed tomography angiography (CTA). Mean distances of the aneurysm neck above the foramen magnum, below the IAC, and above the jugular foramen were 27.0 mm, 3.7 mm, and 8.2 mm, respectively. Distances were all significantly lower versus the comparison group of 9 patients with normal or low-riding PICA aneurysms treated using an FL approach (P < .01). All 6 aneurysms treated using eRS were completely occluded without operative complications.

CONCLUSION
The eRS approach is an important alternative to the FL approach for high-riding PICA aneurysms, identified as having necks more than 23 mm above the foramen magnum on CTA. The glossopharyngo-cochlear triangle is another important anatomic triangle that facilitates microsurgical dissection.
A Case Series of Surgically Treated Spinal Dumbbell Tumors of Critical Parent Nerve Roots: To Cut or Not to Cut?
Authors: Butenschoen Vicki Marie , Kögl Nikolaus , Meyer Bernhard , Thomé Claudius , Wostrack Maria 
Published: December 2020

Abstract

BACKGROUND
Dumbbell tumors present challenging cases, with either an incomplete tumor resection or a need to sacrifice nerve roots. Published literature suggests encouraging neurological outcomes after nerve root amputation.

OBJECTIVE
To determine the incidence of postoperative neurological deficits after amputating the parent nerve root.

METHODS
A retrospective consecutive analysis of all patients treated for dumbbell nerve sheet tumors with a reported amputation of the functional relevant parent nerve roots C5-Th1 and L3-S1.

RESULTS
Among 21 evaluated patients, minor postoperative neurological motor function deterioration occurred in 4 patients (19%). Most patients recovered to the preoperative level at the follow-up examination, and only one patient retained a new Medical Research Council (MRC) scale of 4/5 for deltoid weakness. The majority of tumors were resected at the lumbar level (nerve root L3: 28.6%, L5: 19%). Gross total resection was achieved in 90.5% of patients. Neuropathic pain was reported in one third of the patients during the long-term follow-up.

CONCLUSION
Amputating critical parent nerve roots during the dumbbell tumor resections seems to result in a low incidence of postoperative motor deficits and may offer an acceptable sacrifice in otherwise only incompletely resectable dumbbell tumors. The cross-innervation of neighboring nerve roots and its, probably, per-se-reduced functionality may be a possible mechanism for maintaining motor function.
Retroperitoneal Exposure of the Anterior Lumbar Spine
Authors: Burkett Daniel J , Burkett Jeffrey S 
Published: December 2020

Abstract

BACKGROUND
Anterior lumbar spine procedures such as anterior lumbar interbody fusion (ALIF) are used commonly to treat multiple pathologies, including pseudoarthrosis and degenerative disk disease. It is generally a safe and effective procedure, but an anterior approach to the lumbar spine requires critical navigation of the surgical window to avoid delicate structures. An operative technique should maximize the exposure without an increased risk of iatrogenic injury.

OBJECTIVE
To describe in detail a retroperitoneal exposure of the anterior lumbar spine.

METHODS
This surgical approach is a unique variation of standard anterior lumbar spine exposure techniques. This technique is described and illustrated in detail with an accompanying Supplemental Digital Content: video. Institutional Review Board (IRB) approval was not required because this is a variation of current techniques. Patient consent was obtained for the procedure and use of operative pictures and videos.

RESULTS
Precise details of the technique are described. The surgical video demonstrates the technique for the L5-S1 ALIF approach.

CONCLUSION
This technique is a novel variation of the standard retroperitoneal exposure of the anterior lumbar spine. The incision placement, size, and dynamic blunt retraction of this approach limit tissue disruption and provide an efficient exposure that has not been previously described in the literature.
Fenestration of Symptomatic Ventriculus Terminalis: 2-Dimensional Operative Video
Authors: Helal Ahmed , Pirina Alessandro , Sorenson Thomas J , Palandri Giorgio 
Published: December 2020

Abstract
This is a case of a 73 yr-old female presenting with low back pain and bilateral sciatic pain, which progressed to bilateral lower extremity weakness about 1 yr after initial presentation. Imaging of her thoraco-lumbar spine revealed a ventriculus terminalis extending from the level of the eleventh thoracic vertebra (T11) to the first lumbar vertebra (L1). Ventriculus terminalis is an embryological ependymal lined cavity that normally regresses with development. In individuals with persistent ventriculus terminalis, it is usually incidentally noted during spine imaging for other purposes. Surgery is seldom indicated except for patients presenting with progressive neurological deficits. Patient was taken to surgery for fenestration of the ventriculus and decompression of the spinal canal. The patient was positioned prone, and after proper localization the vertebral column was exposed through a midline approach and a central laminectomy was performed from T11-L1. Intraoperative monitoring was used throughout the procedure. This video illustrates the gross appearance of a ventriculus terminalis, dissection of surrounding neural elements, and decompression of the ventriculus using a wide fenestration technique.
All relevant patient identifiers have been removed from the video. Nevertheless, patient consent was obtained regarding video recording and redistribution of procedure for educational purposes.
Single- versus Dual-Attending Surgeon Approach for Spine Deformity: A Systematic Review and Meta-Analysis
Authors: Lak Asad M , Abunimer Abdullah M , Goedmakers Caroline M W , Aglio Linda S , Smith Timothy R , Makhni Melvin , Mekary Rania A , Zaidi Hasan A 
Published: December 2020

Abstract

BACKGROUND
Surgical management of spine deformity is associated with significant morbidity. Recent literature has inconsistently demonstrated better outcomes after utilizing 2 attending surgeons for spine deformity.

OBJECTIVE
To conduct a systematic review and meta-analysis on studies reporting outcomes following single- vs dual-attending surgeons for spine deformity.

METHODS
MEDLINE, Embase, Web of science, and Cochrane databases were last searched on July 16, 2020. A total of 1013 records were identified excluding duplicates. After screening, 10 studies (4 cohort, 6 case series) were included in the meta-analysis. Random-effect models were used to pool the effect estimates by study design. When feasible, further subgroup analysis by deformity type was conducted.

RESULTS
A total of 953 patients were analyzed. Pooled results from propensity score-matched cohort studies revealed that the single-surgeon approach was unfavorably associated with a nonstatistically significant higher blood loss (mean difference = 421.0 mL; 95% CI: −28.2, 870.2), a statistically significant higher operative time (mean difference = 94.3 min; 95% CI: 54.9, 133), length of stay (mean difference = 0.84 d; 95% CI: 0.46, 1.22), and an increased risk of complications (Mantel-Haenszel risk ratio = 2.93; 95% CI: 1.12, 7.66). Data from pooled case series demonstrated similar results for all outcomes. Moreover, these results did not differ significantly between deformity types (adolescent idiopathic scoliosis and adult spinal deformity).

CONCLUSION
Dual-attending surgeon approach appeared to be associated with reduced operative time, shorter hospital stays, and reduced risk of complications. These findings may potentially improve outcomes in surgical treatment of spine deformity.
Construction, characterization and crystal structure of a fluorescent single-chain Fv chimera
Authors: Velappan Nileena , Close Devin , Hung Li-Wei , Naranjo Leslie , Hemez Colin , DeVore Natasha , McCullough Donna K , Lillo Antonietta M , Waldo Geoffrey S , Bradbury Andrew R M 
Published: December 2020

Abstract
In vitro display technologies based on phage and yeast have a successful history of selecting single-chain variable fragment (scFv) antibodies against various targets. However, single-chain antibodies are often unstable and poorly expressed in Escherichia coli. Here, we explore the feasibility of converting scFv antibodies to an intrinsically fluorescent format by inserting the monomeric, stable fluorescent protein named thermal green, between the light- and heavy-chain variable regions. Our results show that the scTGP format maintains the affinity and specificity of the antibodies, improves expression levels, allows one-step fluorescent assay for detection of binding and is a suitable reagent for epitope binning. We also report the crystal structure of an scTGP construct that recognizes phosphorylated tyrosine on FcεR1 receptor of the allergy pathway.
Smoking during pregnancy: changes and associated risk factors in Spain, 1980-2016
Authors: Salinas-Vilca Ana , Cuevas Lourdes ,   ECEMC Peripheral Group, Bermejo-Sánchez Eva , Galán Iñaki 
Published: December 2020

AbstractBackgroundTrends for maternal smoking rates have varied substantially across industrialized countries. The objective was to describe how the prevalence of maternal smoking evolved in Spain during 1980–2016.MethodsData came from the Spanish Collaborative Study of Congenital Malformations. Our sample consisted of 40 934 mothers of newborns with no congenital defects from hospitals all across Spain. We estimated change points in trend and the mean annual change in smoking prevalence using ‘joinpoint’ regression. Relevant potential factors (age, country of birth, education, parity, planned pregnancy and alcohol consumption) were examined using multivariate logistic regression.ResultsMaternal smoking prevalence in 1980 and 2016 were 14.3% (95% confidence interval [CI]: 11.9–17.0) and 20.4% (95% CI: 15.9–25.8). We identified four periods with distinct trends: a sharp increase during the 80s, a plateau during the 90s, a decrease starting in 2000 and a slowdown of such decrease from 2009 on. Smoking was significantly higher among young women, Spain-born, with low education, unplanned pregnancy, and alcohol consumption.ConclusionsCurrently in Spain maternal smoking remains very high. Tobacco consumption trend showed an increase during the 80s, a plateau during the 90s, and a reduction in the 2000s. Several sociodemographic and behavioural factors were associated to greater likelihood of smoking.
Neurological Soft Signs Are Associated With Altered Cerebellar-Cerebral Functional Connectivity in Schizophrenia
Authors: Cai Xin-Lu , Wang Yong-Ming , Wang Yi , Zhou Han-Yu , Huang Jia , Wang Ya , Lui Simon S Y , Møller Arne , Hung Karen S Y , Mak Henry K F , Sham Pak C , Cheung Eric F C , Chan Raymond C K 
Published: December 2020

Abstract
Cerebellar dysfunction is associated with neurological soft signs (NSS), which is a promising endophenotype for schizophrenia spectrum disorders. However, the relationship between cerebellar-cerebral resting-state functional connectivity (rsFC) and NSS is largely unexplored. Moreover, both NSS and cerebellar-cerebral rsFC have been found to be correlated with negative symptoms of schizophrenia. Here, we investigated the correlations between NSS and cerebellar-cerebral rsFC, explored their relationship with negative symptoms in a main dataset, and validated the significant findings in a replication dataset. Both datasets comprised schizophrenia patients and healthy controls. In schizophrenia patients, we found positive correlations between NSS and rsFC of the cerebellum with the inferior frontal gyrus and the precuneus, and negative correlations between NSS and rsFC of the cerebellum with the inferior temporal gyrus. In healthy controls, NSS scores were positively correlated with rsFC of the cerebellum with the superior frontal gyrus and negatively correlated with rsFC between the cerebellum and the middle occipital gyrus. Cerebellar-prefrontal rsFC was also positively correlated with negative symptoms in schizophrenia patients. These findings were validated in the replication dataset. Our results suggest that the uncoupling of rsFC between the cerebellum and the cerebral cortex may underlie the expression of NSS in schizophrenia. NSS-related cerebellar-prefrontal rsFC may be a potential neural pathway for possible neural modulation to alleviate negative symptoms.