bluuebunny/crossref_metadata_2025_split huggingface dataset:
BSE‐IA reveals retardation mechanisms of polymer powders on cement hydration
Authors: Peng Yu , Zeng Qiang , Xu Shilang , Zhao Guorong , Wang Peiming , Liu Xianping 
Published: December 2019

AbstractThe retardation effects of two polymer powders, styrene butadiene rubber and ethylene‐vinyl acetate, on cement hydration and the mechanisms in terms of hydration degree and microstructure characteristics were investigated by the combination of backscattered electron image analysis, scanning electron microscopy, energy dispersive spectrometer, and X‐ray diffraction. Results show that the cement hydration can be significantly retarded by both polymers with the reductions in cement hydration degrees and hydration products thickness. Polymers retard cement hydration by agglomerating the polymer particles on the surfaces of cement grains, forming films in different thickness and bonding the calcium ions in the pore solutions. Consequentially, the formation of C‐S‐H and CH is depressed and the Ca/Si ratios in the hydration product layer are raised. The retardation is enhanced with the increase in polymer content. This work helps understand the retardation effect of polymers on cement hydration in depth, which enables rational development of polymer‐modified cement‐based materials for further applications.
Brief report: Follow‐up outcomes of multisystemic therapy for adolescents with an intellectual disability and the relation with parental intellectual disability
Authors: Blankestein Annemarieke , Lange Aurelie , van der Rijken Rachel , Scholte Ron , Moonen Xavier , Didden Robert 
Published: December 2019

AbstractResearch on follow‐up outcomes of systemic interventions for family members with an intellectual disability is scarce. In this study, short‐term and long‐term follow‐up outcomes of multisystemic therapy for adolescents with antisocial or delinquent behaviour and an intellectual disability (MST‐ID) are reported. In addition, the role of parental intellectual disability was examined.Outcomes of 55 families who had received MST‐ID were assessed at the end of treatment and at 6‐month, 12‐month and 18‐month follow‐up. Parental intellectual disability was used as a predictor of treatment outcomes. Missing data were handled using multiple imputation.Rule‐breaking behaviour of adolescents declined during treatment and stabilized until 18 months post‐treatment. The presence or absence of parental intellectual disability did not predict treatment outcomes.This study was the first to report long‐term outcomes of MST‐ID. The intervention achieved similar results in families with and without parents with an intellectual disability.
NFAT5 promotes arteriogenesis via MCP‐1‐dependent monocyte recruitment
Authors: Lin Xing‐Chi , Pan Miao , Zhu Ling‐Ping , Sun Quan , Zhou Zheng‐Shi , Li Chuan‐Chang , Zhang Guo‐Gang 
Published: December 2019

AbstractStudies have demonstrated that nuclear factor of activated T cells 5 (NFAT5) is not only a tonicity‐responsive transcription factor but also activated by other stimuli, so we aim to investigate whether NFAT5 participates in collateral arteries formation in rats. We performed femoral artery ligature (FAL) in rats for hindlimb ischaemia model and found that NFAT5 was up‐regulated in rat adductors with FAL compared with sham group. Knockdown of NFAT5 with locally injection of adenovirus‐mediated NFAT5‐shRNA in rats significantly inhibited hindlimb blood perfusion recovery and arteriogenesis. Moreover, NFAT5 knockdown decreased macrophages infiltration and monocyte chemotactic protein‐1 (MCP‐1) expression in rats adductors. In vitro, with interleukin‐1β (IL‐1β) stimulation and loss‐of‐function studies, we demonstrated that NFAT5 knockdown inhibits MCP‐1 expression in endothelial cells and chemotaxis of THP‐1 cells regulated by ERK1/2 pathway. More importantly, exogenous MCP‐1 delivery could recover hindlimb blood perfusion, promote arteriogenesis and macrophages infiltration in rats after FAL, which were depressed by NFAT5 knockdown. Besides, NFAT5 knockdown also inhibited angiogenesis in gastrocnemius muscles in rats. Our results indicate that NFAT5 is a critical regulator of arteriogenesis and angiogenesis via MCP‐1‐dependent monocyte recruitment, suggesting that NFAT5 may represent an alternative therapeutic target for ischaemic diseases.
Imperatorin promotes osteogenesis and suppresses osteoclast by activating AKT/GSK3 β/β‐catenin pathways
Authors: Yan De‐Yi , Tang Jiahao , Chen Liang , Wang Bingzhang , Weng Sheji , Xie Zhongjie , Wu Zong‐Yi , Shen Zijian , Bai Bingli , Yang Lei 
Published: December 2019

AbstractOsteoporosis is caused by disturbance in the dynamic balance of bone remodelling, a physiological process, vital for maintenance of healthy bone tissue in adult humans. In this process, a new bone is formed by osteoblasts and the pre‐existing bone matrix is resorbed by osteoclasts. Imperatorin, a widely available and inexpensive plant extract with antioxidative and apoptotic effects, is reported to treat osteoporosis. However, the underlying mechanism and specific effects on bone metabolism have not been elucidated. In this study, we used rat bone marrow‐derived mesenchymal stem cells and found that imperatorin can activate RUNX2, COL1A1 and osteocalcin by promoting the Ser9 phosphorylation of GSK3β and entry of β‐catenin into the nucleus. Imperatorin also enhanced the production of phospho‐AKT (Ser473), an upstream factor that promotes the Ser9 phosphorylation of GSK3β. We used ipatasertib, a pan‐AKT inhibitor, to inhibit the osteogenic effect of imperatorin, and found that imperatorin promotes osteogenesis via AKT/GSK3β/β‐catenin pathway. Next, we used rat bone marrow‐derived monocytes, to check whether imperatorin inhibits osteoclast differentiation via AKT/GSK3β/β‐catenin pathway. Further, we removed the bilateral ovaries of rats to establish an osteoporotic model. Intragastric administration of imperatorin promoted osteogenesis and inhibited osteoclast in vivo. Our experiments showed that imperatorin is a potential drug for osteoporosis treatment.
An outcome model for human bladder cancer: A comprehensive study based on weighted gene co‐expression network analysis
Authors: Xiong Yaoyi , Yuan Lushun , Xiong Jing , Xu Huimin , Luo Yongwen , Wang Gang , Ju Lingao , Xiao Yu , Wang Xinghuan 
Published: December 2019

AbstractThe precision evaluation of prognosis is crucial for clinical treatment decision of bladder cancer (BCa). Therefore, establishing an effective prognostic model for BCa has significant clinical implications. We performed WGCNA and DEG screening to initially identify the candidate genes. The candidate genes were applied to construct a LASSO Cox regression analysis model. The effectiveness and accuracy of the prognostic model were tested by internal/external validation and pan‐cancer validation and time‐dependent ROC. Additionally, a nomogram based on the parameter selected from univariate and multivariate cox regression analysis was constructed. Eight genes were eventually screened out as progression‐related differentially expressed candidates in BCa. LASSO Cox regression analysis identified 3 genes to build up the outcome model in E‐MTAB‐4321 and the outcome model had good performance in predicting patient progress free survival of BCa patients in discovery and test set. Subsequently, another three datasets also have a good predictive value for BCa patients' OS and DFS. Time‐dependent ROC indicated an ideal predictive accuracy of the outcome model. Meanwhile, the nomogram showed a good performance and clinical utility. In addition, the prognostic model also exhibits good performance in pan‐cancer patients. Our outcome model was the first prognosis model for human bladder cancer progression prediction via integrative bioinformatics analysis, which may aid in clinical decision‐making.
Evaluation of process indicators of a medication review service between pharmacists and physicians
Authors: Marques Tatiane C. , Silva Rafaella de Oliveira Santos , dos Santos Júnior Genival A. , de Jesus Júnior Francisco C. , Silvestre Carina C. , Rocha Kérilin S. S. , da Rocha Chiara E. , Brito Giselle de Carvalho , de Lyra‐Jr Divaldo P. 
Published: December 2019

AbstractRationale, aims and objectivesLiterature have showed inconclusive or contradictory results regarding medication review services effectiveness in optimizing process indicators. Thus, this study aimed to evaluate the process indicators of a medication review service between pharmacists and physicians.MethodThis quasi‐experimental study was conducted between March 2013 and February 2014 with patients who were receiving care in a medication review service in a teaching hospital in northeastern Brazil. The main process indicators were number of pharmaceutical consultations; identification and resolution of drug‐related problems (DRP) and pharmaceutical interventions that were classified according to type and degree of acceptance. Descriptive statistics were used to report data. The statistical significance of the association between variables was evaluated using the Mantel‐Haenszel chi‐square test. The 95% confidence interval was considered, and differences were deemed statistically significant if P ≤ .05.ResultsA total of 146 patients attended the medication review service. The number of consultations per patient ranged from one to five (2.1 ± 1.1). The service identified 366 DRP, most of which were indication (67.5%). Patients who had four to five pharmaceutical consultations were 1.14 times more likely to have their DRP identified (χ2 = 33.83, P < .0001). Of the DRP identified, 183 (42.33%) were resolved. Patients who had between one and two pharmaceutical consultations were 1.22 times more likely not to have their DRP resolved compared with the group with more than three consultations (χ2 = 3.44, P < .05). Of the 173 pharmaceutical interventions made to the medical students and physicians, the majority (98.7%) was accepted.ConclusionThe collaborative medication review service optimized the process indicators. Drug‐related problems identification and resolution required more than three pharmaceutical consultations. Most of the pharmaceutical interventions were accepted by prescribers. Thus, collaborative medication review services may be fundamental to the construction of more effective and safe health systems.
Analysis of factors associated with the prognosis of cirrhotic patients who were treated with tolvaptan for hepatic edema
Authors: Atsukawa Masanori , Tsubota Akihito , Takaguchi Koichi , Toyoda Hidenori , Iwasa Motoh , Ikegami Tadashi , Chuma Makoto , Nozaki Akito , Uojima Haruki , Hiraoka Atsushi , Fukunishi Shinya , Yokohama Keisuke , Tada Toshifumi , Kato Keizo , Abe Hiroshi , Tani Joji , Okubo Hironao , Watanabe Tsunamasa , Hattori Nobuhiro , Tsutsui Akemi , Senoh Tomonori , Yoshida Yuji , Okubo Tomomi , Itokawa Norio , Nakagawa‐Iwashita Ai , Kondo Chisa , Arai Taeang , Michitaka Kojiro , Iio Etsuko , Kumada Takashi , Tanaka Yasushito , Takei Yoshiyuki , Iwakiri Katsuhiko 
Published: December 2019

AbstractBackground and AimThe prognosis of cirrhotic patients with hepatic edema is poor. Although several short‐term predictors of tolvaptan (novel diuretic agent) treatment for such patients have been reported, the factors related to long‐term survival are still unclear.MethodsAmong 459 patients with hepatic edema enrolled in a retrospective, multicenter collaborative study, we analyzed 407 patients who received tolvaptan.ResultsPatients consisted of 266 men and 141 women, with the median age of 68 years (range, 28–93 years). The frequency of short‐term responders to tolvaptan was 59.7% (243/407). In the Cox regression analysis, short‐term response to tolvaptan, low average dosages of furosemide and spironolactone during tolvaptan treatment, Child–Pugh classification A and B, and absence of hepatocellular carcinoma were independent factors contributed to 1‐year survival. The 1‐year and long‐term cumulative survival rates in short‐term responders were significantly higher than those in non‐responders (P = 0.011 and 0.010, respectively). Using a receiver operating characteristic curve analysis, the optimal cut‐off values of average daily dosages of furosemide and spironolactone for predicting 1‐year survival were 19 and 23 mg/day, respectively. The long‐term cumulative survival rates in patients who received a mean dosage of spironolactone < 23 mg/day during tolvaptan treatment were significantly higher than those receiving a mean dosage of ≥ 23 mg/day (P = 0.001).ConclusionsThe present study suggests that the short‐term response to tolvaptan and low dosages of conventional diuretics during tolvaptan treatment might improve the 1‐year and long‐term survival rates in cirrhotic patients with hepatic edema.
Direct investigation of current transport in cells by conductive atomic force microscopy
Authors: ZHAO W. , CHEONG L.‐Z. , XU S. , CUI W. , SONG S. , ROURK C.J. , SHEN C. 
Published: December 2019

SummaryCurrents play critical roles in neurons. Direct observation of current flows in cells at nanometre dimensions and picoampere current resolution is still a daunting task. In this study, we investigated the current flows in hippocampal neurons, PC12 cells and astrocytes in response to voltages applied to the cell membranes using conductive atomic force microscopy (CAFM). The spines in the hippocampal neurons play crucial roles in nerve signal transfer. When the applied voltage was greater than 7.2 V, PC12 cells even show metallic nanowire‐like characteristics. Both the cell body and glial filaments of astrocytes yielded CAFM test results that reflect different electrical conductance. To our best knowledge, the electrical characteristics and current transport through components of cells (especially neurons) in response to an applied external voltage have been revealed for the first time at nanometre dimensions and picoampere current levels. We believe that such studies will pave new ways to study and model the electrical characteristics and physiological behaviours in cells and other biological samples.
Relating ligand binding to activation gating in P2X2 receptors using a novel fluorescent ATP derivative
Authors: Sattler Christian , Schmauder Ralf , Schwabe Tina , Schweinitz Andrea , Unzeitig Christopher , Schwede Frank , Otte Maik , Benndorf Klaus 
Published: December 2019

AbstractIonotropic purinergic receptors (P2X receptors) are non‐specific cation channels that are activated by the binding of ATP at their extracellular side. P2X receptors contribute to multiple functions, including the generation of pain, inflammation, or synaptic transmission. The channels are trimers and structural information on several of their isoforms is available. In contrast, the cooperation of the subunits in the activation process is poorly understood. We synthesized a novel fluorescent ATP derivative, 2‐[DY‐547P1]‐AET‐ATP (fATP) to unravel the complex activation process in P2X2 and mutated P2X2 H319K channels with enhanced apparent affinity by characterizing the relation between ligand binding and activation gating. fATP is a full agonist with respect to ATP that reports the degree of binding by bright fluorescence. For quantifying the binding, a fast automated algorithm was employed on human embryonic kidney cell culture images. The concentrations of half maximum occupancy and activation as well as the respective Hill coefficients were determined. All Hill coefficients exceeded unity, even at an occupancy <10%, suggesting cooperativity of the binding even for the first and second binding step. fATP shows promise for continuative functional studies on other purinergic receptors and, beyond, any other ATP‐binding proteins.
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A case of cervical adenocarcinoma arising from endometriosis in the absence of human papilloma virus infection
Authors: Uehara Takashi , Yoshida Hiroshi , Kondo Atsushi , Kato Tomoyasu 
Published: December 2019

AbstractEndometriosis‐related cervical neoplasms are rare, and their clinicopathological features and association with human papilloma virus infection are unclear. A postmenopausal woman with recently diagnosed cervical adenocarcinoma was referred to our hospital. After further investigation, we suspected a stage IIB neoplasm that originated from endometriosis of the cervix or left parametrium. A radical hysterectomy was performed, and pathological examination confirmed a stage IIB cervical endometrioid carcinoma arising from the endometriosis in the cervix to the left parametrium that invaded the cervix; human papilloma virus infection of tumor cells was not detected. Endometriosis‐related neoplasms can occur in the cervix or parametrium and present as cervical cancer. The neoplasm described here was not associated with human papilloma virus infection.
Characteristics and influence of Mycoplasma/Ureaplasma cultures in amniotic fluid on perinatal outcomes
Authors: Takakura Sho , Kodama Yuki , Yamashita Rie , Kino Emi , Kawano Noriko , Tomimori Kayo , Maki Yohei , Doi Koutarou , Kaneko Masatoki , Sameshima Hiroshi 
Published: December 2019

AbstractAimTo investigate the effects of Mycoplasma/Ureaplasma cultured in amniotic fluid on perinatal characteristics in preterm delivery between 22 and 33 weeks of gestation.MethodsThe study was conducted in a tertiary perinatal center and involved 38 pregnant women who had undergone amniocentesis to evaluate intrauterine infection due to preterm labor or premature rupture of membranes. The subjects were divided into three groups based on the culture results: negative (Negative Group, n = 24), positive for Mycoplasma/Ureaplasma (M/U Group, n = 6), and positive for other pathogens (Other Pathogens Group, n = 8). One‐way analysis of variance was used to compare the three groups.ResultsThe incidence of histological chorioamnionitis and neonatal sepsis was significantly different among the three groups (the Negative Group and the Other Pathogens Group, P < 0.01; the M/U Group and the Other Pathogens Group, P = 0.03). In the M/U Group, no infants had sepsis, severe intraventricular hemorrhage, cystic periventricular leukomalacia, or poor neurological outcomes, but one infant developed bronchopulmonary dysplasia and needed home oxygen treatment. Although one died of gastrorrhexis, the remaining five patients had normal brain magnetic resonance imaging findings and developed normally.ConclusionThe presence of Mycoplasma/Ureaplasma isolated from amniotic fluid did not cause neonatal sepsis or poor prognosis. In some infants, there was no histological chorioamnionitis in the placenta. These pathogens thus seem to be less invasive than any other microbes with respect to perinatal outcomes.
Digital psychosocial assessment: An efficient and effective screening tool
Authors: Ho Jane , Fong Cameron K , Iskander Andrew , Towns Susan , Steinbeck Katharine 
Published: December 2019

AimA large proportion of young people with chronic health conditions are surviving into adulthood. They face the same challenges as their healthy peers and are at increased risk of mental health problems. Psychosocial assessment is a crucial aspect of clinical care. Interviews using the internationally used and accepted HEEADSSS (home environment, education/employment, eating, peer‐related activities, drugs, sexuality, suicide/depression, and safety) framework require trained clinicians, rapid interpersonal engagement, time and manual documentation. HEEADSSS‐derived digital self‐report surveys can be initiated by non‐trained staff. This study compares the utility and information recorded using both methods.MethodsA retrospective analysis comparing documentation from HEEADSSS guided face‐to‐face interview and a digital survey tool was conducted using 146 records collected by the Trapeze transition service across the two locations of the Sydney Children's Hospital Network (NSW, Australia) between 2013 and 2016. A panel of four experts used an iterative process to identify 29 data verification points, falling into seven categories. Wilcoxon signed‐rank tests were used to compare category scores.ResultsThe digital survey took an average of 15 min and showed a significantly higher rate of disclosure across all psychosocial categories, particularly in the sensitive areas of emotions, drug use, sex and safety, compared to electronic medical record documentation of interview.ConclusionsDigital survey provided a time‐efficient psychosocial screening tool that was self‐administered, able to be introduced by non‐trained staff, had a consistent record of responses, and elicited a substantially higher disclosure rate for important areas of strength and risk that may otherwise be avoided or not recorded.
Top 10 research priorities for human milk banking and use of donor human milk: A partnership between parents and clinicians
Authors: Matthews Eliza , Collins Carmel T , Ellison Vanessa , Hussey Lee , Slade Jo , Keir Amy , Miller Jacqueline 
Published: December 2019

AimThis study sought to establish research priorities in human milk banking and use of pasteurised donor human milk. It aimed to (i) collaborate with national stakeholders, including parents of preterm infants, human milk donors and health‐care professionals, to identify evidence uncertainties and (ii) agree by consensus on a consolidated prioritised list of research questions.MethodsA consensus approach modelled on the James Lind Alliance was used. A steering group was formed, and key stakeholder organisations identified. Evidence uncertainties were gathered through an online survey and literature search. An iterative process was used to consolidate and rank questions. A final workshop was held to identify the top 10 research priorities.ResultsA total of 391 evidence uncertainties were gathered from 202 respondents (38% parents of preterm infants/milk donors, 50% health‐care professionals, 12% who identified as both a parent of preterm and health professional) and a further 15 were identified from literature. The steering group consolidated these to 39 evidence uncertainties, which were ranked via another survey. The top 24 questions were workshopped by 13 participants (four parents and nine clinicians) to determine the top 10 research priorities. These included the risks and benefits of using donor milk, optimum techniques for processing and the effects of these on the properties of milk, the nutrient profile of the milk and clinical criteria for prioritising receipt of milk.ConclusionThe top 10 research priorities in human milk banking and use of donor milk were identified and can be used to guide researchers.