bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication and Performance of Atmospheric Plasma Spraying Solid Oxide Fuel Cells with Liquid Antimony Anodes
Authors: Jiang Yidong , Mo Wenfei , Cao Tianyu , Shi Yixiang , Cai Ningsheng 
Published: July 2020

Abstract
Solid oxide fuel cells (SOFCs) with liquid antimony anodes (LAAs) are potential energy conversion technology for impurity-containing fuels. Atmospheric plasma spraying (APS) technology has become a promising LAA-SOFC preparation method because of its economy and convenience. In this paper, button SOFCs with different cathode materials and pore former ratio were prepared by the APS method and measured at 750℃. The effect of cathode structure on the electrochemical performance of the LAA-SOFCs was analyzed, and an optimized spraying method for LAA-SOFCs was developed. A tubular LAA-SOFC was prepared by the APS method based on the optimized spraying method and a peak power of 2.5 W was reached. The tubular cell was also measured at a constant current of 2 A for 20 hours and fed with a sulfur-containing fuel to demonstrate the impurity resistance and the long-term stability.
Neoadjuvant systemic and hyperthermic intraperitoneal chemotherapy combined with cytoreductive surgery for gastric cancer patients with limited peritoneal metastasis: a prospective cohort study
Authors: Yu Pengfei , Ye Zeyao , Dai Gaiguo , Zhang Yanqiang , Huang Ling , Du Yian , Cheng Xiangdong 
Published: July 2020

Abstract
Background
There is no currently available treatment for peritoneal metastasis of gastric cancer. This phase II study aimed to evaluate the efficacy and safety of neoadjuvant systemic chemotherapy and hyperthermic intraperitoneal chemotherapy (HIPEC) combined with cytoreductive surgery (CRS) for the treatment of these patients.
Methods
Neoadjuvant chemotherapy comprised two cycles of HIPEC and four cycles of S-1 plus paclitaxel. HIPEC was administered intraperitoneally with paclitaxel (75 mg/m2). For systemic chemotherapy, paclitaxel was administered intravenously(150 mg/m2) on day 1,and S-1 was administered orally(80 mg/m2/day)on days 1–14 of a 3-week cycle. Another two cycles of HIPEC and four cycles of S-1 plus paclitaxel were administered after second diagnostic staging laparoscopy or CRS. The primary endpoint was treatment efficiency and safety; the secondary endpoint was 3-year overall survival (OS).
Results
A total of 40 patients were enrolled and 38 patients have been analyzed. Of these, 18 (47.4%) patients received neoadjuvant systemic chemotherapy, HIPEC and CRS (conversion therapy group), while 20 patients received only chemotherapy and HIPEC (palliative chemotherapy group). Median OS was markedly improved in the conversion therapy group (21.1 months, 95% confidence interval [CI] 16.7–25.6 months) in comparison with the palliative chemotherapy group(10.8 months, 95%CI 7.3–14.2 months, p = 0.002). After neoadjuvant systemic chemotherapy and HIPEC, a second laparoscopic exploration was performed, and the prognosis of patients with low peritoneal cancer index (PCI) (PCI < 6) was significantly better than that of patients with high PCI (PCI ≥ 6)(20.1 vs.11.3 months, p = 0.006).
Conclusion
Neoadjuvant systemic chemotherapy and HIPEC combined with CRS was safe and feasible, and could potentially improve the prognosis of gastric cancer patients with limited peritoneal metastasis. However, further clinical trials are still warranted.
Trial registration:
This study is registered with ClinicalTrials.gov as NCT02549911, registered on 15 September 2015.
Integration of RNA-Seq and proteomics data identifies glioblastoma multiforme surfaceome signature
Authors: Syafruddin Saiful Effendi , Nazarie Wan Fahmi Wan Mohamad , Moidu Nurshahirah Ashikin , Soon Bee Hong , Mohtar M. Aiman 
Published: July 2020

Abstract
BackgroundGlioblastoma multiforme (GBM) is a highly lethal, stage IV brain tumor with a prevalence of approximately 2 per 10000 people globally. The cell surface proteins or surfaceome play significant roles in modulating cancer phenotypes and acting as information gateway in many oncogenic signaling pathways. Hence, surfaceome are attractive targets for cancer therapy due to their direct accessibility to drugs. Nonetheless, a comprehensive GBM surfaceome landscape has not been fully defined. Thus, the aim of this study is to define GBM-specific surfaceome genes and identify key cell surface genes that could potentially be developed as novel GBM biomarkers, or therapeutically targeted.MethodsWe integrated the RNA-Seq data from TCGA GBM (n=166) and GTEx normal brain cortex (n=408) databases to identify the significantly dysregulated surfaceome in GBM. This was followed by integrative analysis that combines transcriptomics, proteomics and protein-protein interaction network data to prioritize the high-confidence GBM surfaceome signature.ResultsAmong the 2,381 significantly dysregulated genes in GBM, 395 genes were classified as surfaceome. Via the integrative analysis, we identified 6 high-confidence GBM molecular signature, HLA-DRA, CD44, SLC1A5, EGFR, ITGB2, PTPRJ, which were significantly upregulated in GBM. The expression of these genes were validated in an independent transcriptomics database, which confirmed their upregulated expression in GBM. Importantly, high expression of CD44, PTPRJ and HLA-DRA is significantly associated with poor disease-free survival. Lastly, using the Drugbank database, we identified several clinically-approved drugs targeting the GBM molecular signature suggesting potential drug repurposing.ConclusionsIn summary, we identified and highlighted the key GBM surface-enriched repertoires that could be biologically relevant in supporting GBM pathogenesis. These genes could be further interrogated experimentally in future studies that could lead to efficient GBM diagnostic/prognostic markers or a therapeutic regimen to treat GBM.
The Ecological Cost Of Consumption
Authors: Peterson Isaac , Selinske Matthew , Lenzen Manfred , Moilanen Atte 
Published: July 2020

Abstract
The link between global ecosystem decline, trade, and human consumption suggests that trade-based biodiversity footprints should be regarded as a critical indicator of planetary impacts. Here we integrate a global input-output economic framework that encompasses global trade between 15909 sectors, with range and impact data on 10518 terrestrial plant, 17234 terrestrial animal, 6101 freshwater and 5059 marine species, to specify the biodiversity footprints associated with global trade and consumption across domestic and international supply chains. Our framework characterises global species loss as driven by domestic trade in emerging market economies including China, Brazil, Mexico, India, and Ecuador, and exacerbated by consumption in high-income countries, especially those in the G7, that are driving species loss in emerging markets and low-income nations. We attribute the largest sector-scope footprints to construction in China, Colombia, and India, agriculture commodity trade in Madagascar, Mexico, Tanzania, and Peru, and food manufacture in Mexico, Germany and France.
Vagal Afferent Fibers Contribute to the Anti-Inflammatory Reactions by Vagus Nerve Stimulation in Concanavalin A Model of Hepatitis in Rats
Authors: Jo Byung Gon , Namgung Uk 
Published: July 2020

Abstract
Background: Increasing number of studies provide evidence that the vagus nerve stimulation (VNS) dampens inflammation in peripheral visceral organs. However, the effects of afferent fibers of the vagus nerve (AFVN) on anti-inflammation have not been clearly defined. Here, we investigate whether AFVN are involved in VNS-mediated regulation of hepatic production of proinflammatory cytokines. Methods: An animal model of hepatitis was generated by intraperitoneal injection of concanavalin A (ConA) into rats, and electrical stimulation was given to the hepatic branch of the vagus nerve. AFVN was selectively eliminated by treating animals with capsaicin (CAP). mRNA and protein expression in target tissues was analyzed by RT-PCR, real-time PCR, western blotting and immunofluorescence staining.Results: TNF-α, IL-1β, and IL-6 mRNAs and proteins that were induced by ConA in the liver macrophages were significantly reduced by the electrical stimulation of the hepatic branch of the vagus nerve (hVNS). hVNS also induced an increase in phospho-STAT3 in the liver macrophages of ConA-injected animals, suggesting the activation of STAT3 transcription factor associated with cholinergic anti-inflammation. Capsaicin (CAP) treatment deteriorated transient receptor potential vanilloid 1 (TRPV1)-positive neurons and also induced an increase in apoptotic caspase-3 signals in nodose ganglion (NG) neurons. Concomitantly, CAP suppressed choline acetyltransferase (ChAT) expression that was induced by hVNS in DMV neurons of ConA-injected animals. Furthermore, hVNS-mediated downregulation of TNF-α, IL-1β, and IL-6 expression was reduced by CAP administration. Conclusions: Our data indicate that the activity of AFVN contributes to hepatic anti-inflammatory responses mediated by hVNS in ConA model of hepatitis in rats.
Long Lasting Insecticide-treated Bed Net Ownership, Utilization and Associated Factors Among School-age Children in Dara Mallo and Uba Debretsehay Districts, Southern Ethiopia
Authors: Zerdo Zerihun , Bastiaens Hilde , Anthierens Sibyl , Massebo Fekadu , Masine Matios , Biresawu Gelila , Shewangizaw Misgun , Tunje Abayneh , Chisha Yilma , Yohannes Tsegaye , geertruyden Jean-Pierre Van 
Published: July 2020

Abstract
Background
Malaria is one of the major causes of morbidity and mortality among school-aged children (SAC) in Sub-Saharan Africa. SAC account for more than 60% of the reservoir of malaria transmission, but they were given less emphasis on prioritizing malaria prevention interventions. This study was aimed at assessing the ownership of bed nets, its utilization and factors associated with ownership of bed nets by households and bed net utilization among SAC in malaria prone areas of Dara Mallo and Uba Debretsehay districts in Southern Ethiopia, October to December 2019.
Methods
This study is part of baseline assessment in a cluster randomized controlled trial. The data were collected through interview and observation following structured questionnaire from 2261 SAC households. Univariable and multivariable logistic regressions were used to assess the association between bed net ownership or utilization and potential predictor variables. Odds Ratio (OR) and corresponding 95% Confidence Interval (CI) were used to determine the strength and statistical significance of association.
Results
The ownership of at least one bed net by households of SAC was about 19.3% (95% CI: 17.7%-21.0%). It was affected by living in altitude ranged from 1100 to 1250 meters above sea level with Adjusted OR (AOR) of 0.5 (95%CI: 0.3–0.9) and presence of child age less than 5 years (AOR = 2.1; 95%CI: 1.2–3.7). About 7.8% (95% CI: 6.7%-10.0%) of all SAC participated in the study and 40.4% (95% CI: 57.4% − 66.7%) of children in households owning at bed net passed the previous night under bed net. Bed net utilization by SAC conditional to its presence was lower among children age > 9 years (AOR = 0.2; 95%CI: 0.03–0.8); higher in children with mothers who had an education level above grade six (AOR = 5.2; 95%CI: 1.8–17.0); and when the household size to bed net ratio was ≤ 2 (AOR = 37.8; 95%CI: 4.1–1106).
Conclusion
Ownership of bed net was lower than universal coverage of at least one bed net for two individuals. It is important to monitor replacement needs and educate mothers with low education level and their SAC on the benefit of consistent utilization of bed nets.
Identification of Atypical Mitogen-Activated Protein Kinase MAPK4 as A Novel Regulator in Acute Lung Injury
Authors: Mao Ling , Zhou Ya , Chen Longqing , Hu Lin , Liu Shiming , Zheng Wen , Zhao Juanjuan , Guo Mengmeng , Chen Chao , He Zhixu , Xu Lin 
Published: July 2020

Abstract
Background: Acute lung injury (ALI) is a serious disease with highly morbidity and mortality that causes serious health problems worldwide. Atypical mitogen activated protein kinases (MAPKs) play critical roles in the development of tissues and have been proposed as promising therapeutic targets for various diseases. However, the potential role of atypical MAPKs in ALI remains elusive. In this study, we investigated the role of atypical MAPKs family member MAPK4 in ALI using LPS-induced murine ALI model. Results: We found that MAPK4 deficiency mice exhibited prolonged survival time after LPS challenge, accompanied by alleviated pathology in lung tissues, decreased levels of pro-inflammatory cytokines and altered composition of immune cells in BALF. Furthermore, the transduction of related signaling pathways, including MK5, AKT, JNK, and p38 MAPK pathways, was reduced obviously in LPS-treated MAPK4-/- mice. Notably, the expression of MAPK4 was up-regulated in lung tissues of ALI model, which was not related with MAPK4 promoter methylation, but negatively orchestrated by transcriptional factors NFKB1 and NR3C1. Further studies have shown that the expression of MAPK4 was also increased in LPS-treated macrophages. Meanwhile, MAPK4 deficiency reduced the expression of related pro-inflammatory cytokines in macrophage in response to LPS treatment. Finally, MAPK4 inhibition using shRNA pre-treatment could ameliorate the pathology of lung tissues and prolong the survival time of mice after LPS challenge. Conclusions: Collectively, these findings reveal an important biological function of atypical MAPK in mediating the pathology of ALI, indicating that MAPK4 might be a novel potential therapeutic target for ALI treatment.
The Path Linking Disease Severity and Cognitive Function with Quality of Life in Parkinson's Disease: the Mediating Effect of Activities of Daily Living and Depression.
Authors: He Yao , Tian Yuling , Han Hongjuan , Cui Jing , Ge Xiaoyan , Qin Yao , Luo Yanhong , Yu Hongmei 
Published: July 2020

Abstract
Background: Research on quality of life (QOL) with Parkinson’s disease (PD) has examined direct influencing factors, not mediators. The study aim was to explore whether PD severity and poor cognitive function may decrease physical and mental QOL by reducing activities of daily living (ADL) and increasing depression in sequence. Methods: We conducted a cross-sectional questionnaire study of 150 PD hospital patients in China. PD severity, cognitive function, ADL, depression, and QOL were evaluated. We used structural equation modeling to analyze the mediating effects of ADL and depression on the association between PD severity/cognition and physical (PCS) and mental (MCS) QOL. Results:There was a significant mediating effect of PD severity on PCS via ADL and depression (95%CI: −0.669, −0.026), and a significant direct effect (p<0.001). The mediating effect of PD severity on MCS via ADL and depression was significant (95%CI: −2.135, −0.726), but there was no direct effect (p = 0.548). There was a significant mediating effect of cognitive function on PCS via ADL and depression (95%CI: 0.025, 0.219) and a significant direct effect (p<0.001). The mediating effect of cognitive function on MCS via ADL and depression was significant (95%CI: 0.256, 0.645), but there was no direct effect (p = 0.313). The PCS models showed a partial mediation, and the MCS models showed a complete mediation, of ADL and depression.Conclusions:PD severity and cognitive function increase depression by reducing ADL, leading to lower QOL, and directly or indirectly affect PCS and MCS through different pathways.
Role of IL-22 in Persistent Allergic Airway Diseases Caused by House Dust Mite: A Pilot Study
Authors: Tamasauskiene Laura , Gintauskiene Vilte Marija , Bastyte Daina , Sitkauskiene Brigita 
Published: July 2020

Abstract
Background. Persistent allergic airway diseases cause a great burden worldwide. Their pathogenesis is not clear enough. There is evidence that one of the recently described cytokine interleukin (IL) 22 may be involved in the pathogenesis of these diseases. Scientists argue if this cytokine acts as proinflammatory or anti-inflammatory agent. The aim of this study was to investigate IL-22 level in patients with persistent allergic airway diseases caused by house dust mite (HDM) in comparison with healthy individuals and to evaluate its relationship with IL-13 and IL-10 level, symptoms score and quality of life.Methods. Patients with persistent allergic rhinitis caused by HDM and having symptoms for at least 2 years with or without allergic asthma were involved into the study. Measurements of IL-22, IL-13 and IL-10 and in serum and nasal lavage was performed by ELISA. Questionnaires assessing symptoms severity and quality of life were used.Results. A tendency was observed that IL-22 in serum and nasal lavage was higher in patients with allergic airway diseases compared to control group (14.86 pg/ml vs. 7.04 pg/ml and 2.67 pg/ml vs. 1.28 pg/ml, respectively). Positive statistically significant correlation was estimated between serum IL-22 and serum IL-10 (rs=0.57, p<0.01) and IL-13 (rs=0.44, p<0.05) level. Moreover, positive significant correlation was found between IL-22 in nasal lavage and IL-10 in nasal lavage (rs=0.37, p<0.05). There was a negative statistically significant correlation between serum IL-22 and RQLQ (rs=-0.42, p<0.05).Conclusion. Our study showed a possible anti-inflammatory effect of IL-22 in patients with persistent allergic airway diseases caused by HDM.
Plasmodium Falciparum Multidrug Resistance Gene-1 N86Y-Y184F-D1246Y Polymorphisms in Northern Nigeria: Implications for the Continued Use of Artemether-Lumefantrine in the Region
Authors: Adamu Auwal , Jada Mahmoud Suleiman , Haruna Hauwa Mohammed Sani , Yakubu Bassa Obed , Ibrahim Mohammed Auwal , Balogun Emmanuel Oluwadare , Inaoka Daniel Ken , Kita Kiyoshi , Hirayama Kenji , Culleton Richard , Shuaibu Mohammed Nasir 
Published: July 2020

Abstract
Background
The analysis of single nucleotide polymorphism (SNPs) in drug-resistance associated genes is a commonly used strategy for the surveillance of antimalarial drug resistance in populations of parasites. The present study was designed and performed to provide genetic epidemiological data of the prevalence of N86Y-Y184F-D1246Y SNPs in Plasmodium falciparum multidrug resistance 1 (pfmdr1) in the malaria hotspot of Northern Nigeria.
Methods
Plasmodium falciparum-positive blood samples on Whatman-3MM filter papers were collected from 750 symptomatic patients from four states (Kano, Kaduna, Yobe and Adamawa) in Northern Nigeria, and genotyped via BigDye (v3.1) terminator cycle sequencing for the presence of three SNPs in pfmdr1. SNPs in pfmdr1 were used to construct NYD, NYY, NFY, NFD, YYY, YYD, YFD and YFY haplotypes, and all data were analyzed using Pearson Chi-square and Fisher's exact (FE) tests.
Results
The prevalence of the pfmdr1 86Y allele was highest in Kaduna (12.5%, 𝜒² = 10.47, P < 0.05), whilst the 184F allele was highest in Kano (73.1%, 𝜒² = 13.20, P < 0.05), and the pfmdr1 1246Y allele was highest in Yobe (5.26%, 𝜒² = 9.18, P < 0.05). The NFD haplotype had the highest prevalence of 69.81% in Kano (𝜒² = 36.05, P < 0.05), followed by NYD with a prevalence of 49% in Adamawa, then YFD with prevalence of 11.46% in Kaduna. The YYY haplotype was not observed in any of the studied states.
Conclusion
The present study shows that P. falciparum strains with reduced sensitivity to artemether-lumefantrine exist in Northern Nigeria and predominate in the North-West region.
Comparison of adherence and persistence between fixed and unfixed topical combination glaucoma therapies using Japanese healthcare/pharmacy claims database: a retrospective non-interventional cohort study
Authors: Shirai Chikako , Matsuoka Nobushige , Nakazawa Toru 
Published: July 2020

Abstract
Background Adherence and persistence with chronic therapies is crucial to prevent disease progression, such as in glaucoma. However, only a limited number of studies have reported them using real-world data in Japan. This study aimed to evaluate Japanese patients’ adherence and persistence to fixed- and unfixed-combination eye drops as a second-line therapy for glaucoma in real-world practice.Methods This retrospective, non-interventional cohort study utilized a commercially available Japanese healthcare database (MinaCare database). Medical/pharmacy claims data were collected from 2011 to 2016. The primary endpoint was adherence to medications, assessed by proportion of days covered (PDC) with medication during a 12-month post-index period. Meanwhile, the secondary endpoints included the persistence rate.Results A total of 738 patients were included in this study: 309 and 329 in the fixed- and unfixed-combination cohorts, respectively. Prostaglandin analog (PG)/β-blocker (BB) was most commonly claimed in 241/309 (78.0%) patients in the fixed-combination cohort. In the unfixed-combination cohort, PG and BB were claimed in 130/329 (39.5%) patients, whereas PG and α2-agonist were claimed in 87/329 (26.4%) patients. Patients were more adherent to the fixed-combination than the unfixed combinations (mean PDCs [SD], 79.1% [32.1] vs. 62.2% [38.0]; P < 0.0001). The proportion of patients with good adherence (PDC ≥ 80%) was also higher in the fixed-combination cohort (69.6%) than in the unfixed-combination cohort (48.6%) (P < 0.0001). During the 12-month post-index period, the persistence rate was higher in the fixed-combination cohort than in the unfixed-combination cohort (47.6% [95% confidence intervals (CI): 41.9–53.0] vs. 24.9% [95% CI: 20.4–29.7], P < 0.0001).Conclusions Japanese patients with glaucoma preferred the fixed-combination therapies over the unfixed-combination therapies. Hence, fixed-combination therapies would contribute to the improvement of adherence.
RASSF1A Regulates the Abnormal Cell Proliferation in Psoriasis via Inhibition of YAP
Authors: Jia Jinjing , Wang Ning , Zheng Yan , Mo Xiumei , Zhang Yu , Ye Siqi , Liu Junfeng , Yan Fenggen , Li Hongyi , Chen Dacan 
Published: July 2020

Abstract
Background: Psoriasis is a chronic, inflammatory skin disease with high incidence, treatment resistance, and high recurrence. Currently, the exact etiology and pathogenesis are unclear. The goal of this study was to characterize the role of the upstream negative regulator RAS-association domain family 1A (RASSF1A) on Yes-associated protein (YAP) in psoriasis.Methods: Skin lesions of 22 psoriasis patients and 19 normal skin tissue controls were used. Human epidermal keratinocytes were stimulated with M5 (IL-1 α, IL-17, IL-22, TNF-α, oncostatin M), to establish the psoriatic cell model. Methylation inhibitor, 5-Aza-CdR was prepared at different concentrations (5, 10, 20 μmol/L). Cells were infected with lentivirus vector overexpressing RASSF1A. Twenty-five 6-8-week-old female BALB/c mice were used to establish the psoriatic mouse model. Mice were randomly divided into five groups: control (Vaseline applied daily), psoriasis (imiquimod applied daily), and the three different 5-Aza-CdR concentrations (applied daily with imiquimod). Methylation specific PCR (MSP) was used to detect RASSF1A methylation, and immunohistochemistry was used to detect RASSF1A expression in skin lesions. After adding 5-Aza-CdR or lentivirus vector overexpressing RASSF1A, YAP expression, cell proliferation, cell cycle, apoptosis, inflammatory cytokines and related signal pathway activity were detected.Results: As RASSF1A methylation level increased, its expression in psoriasis patients and mice with skin lesions decreased. Addition of 5-Aza-CdR or lentivirus vector overexpressing RASSF1A increased the expression of RASSF1A, reduced the expression of YAP and inflammatory cytokines, cell proliferation, as well as AKT, ERK, STAT3 and NF-κB signaling pathway activities, induced cell cycle arrest in G0/G1 phase, increased apoptosis, and improved skin lesions.Conclusions: RASSF1A inhibited the proliferation of psoriatic cells, induced apoptosis, and reduced the expression of inflammatory factors by inhibiting YAP expression. Based on our findings, targeted drugs that can inhibit RASSF1A methylation and increase its expression may be useful in the treatment of psoriasis.