bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and functional characterization of ABCC transporters for Cd tolerance and accumulation in Sedum alfredii Hance
Authors: Feng Tongyu , He Xuelian , Zhuo Renying , Qiao Guirong , Han Xiaojiao , Qiu Wenmin , Chi Linfeng , Zhang Dayi , Liu Mingying 
Published: December 2020

AbstractCd is one of the potential toxic elements (PTEs) exerting great threats on the environment and living organisms and arising extensive attentions worldwide. Sedum alfredii Hance, a Cd hyperaccumulator, is of great importance in studying the mechanisms of Cd hyperaccumulation and has potentials for phytoremediation. ATP-binding cassette sub-family C (ABCC) belongs to the ABC transporter family, which is deemed to closely associate with multiple physiological processes including cellular homeostasis, metal detoxification, and transport of metabolites. In the present work, ten ABCC proteins were identified in S. alfredii Hance, exhibiting uniform domain structure and divergently clustering with those from Arabidopsis. Tissue-specific expression analysis indicated that some SaABCC genes had significantly higher expression in roots (Sa23221 and Sa88F144), stems (Sa13F200 and Sa14F98) and leaves (Sa13F200). Co-expression network analysis using these five SaABCC genes as hub genes produced two clades harboring different edge genes. Transcriptional expression profiles responsive to Cd illustrated a dramatic elevation of Sa14F190 and Sa18F186 genes. Heterologous expression in a Cd-sensitive yeast cell line, we confirmed the functions of Sa14F190 gene encoding ABCC in Cd accumulation. Our study performed a comprehensive analysis of ABCCs in S. alfredii Hance, firstly mapped their tissue-specific expression patterns responsive to Cd stress, and characterized the roles of Sa14F190 genes in Cd accumulation.
Insights into genomic structure and evolutionary processes of coastal Suaeda species in East Asia using cpDNA, nDNA, and genome-wide SNPs
Authors: Park Jong-Soo , Jin Dong-Pil , Choi Byoung-Hee 
Published: December 2020

AbstractSpecies in the genus Suaeda have few diagnostic characters and substantial morphological plasticity. Hence, regional floras do not provide clear taxonomic information for Suaeda spp. in East Asia. In order to assess the taxonomy of four species in the genus Suaeda (S. australis, S. maritima, S. japonica, and S. heteroptera), cpDNA (rpl32-trnL and trnH-psbA), nDNA (ITS), and MIG-seq analyses were carried out. Genome-wide SNP results indicated three lineages: (1) S. australis in Korea and S. maritima in Japan, (2) S. maritima in Korea and S. heteroptera in China, and (3) S. japionica. In phylogenetic trees and genotype analyses, cpDNA and nDNA results showed discrepancies, while S. japonica and S. maritima in Korea, and S. heteroptera in China shared the same haplotype and ribotype. We suggest that the shared haplotype may be due to chloroplast capture. Based on our results, we assume that S. japonica was formed by homoploid hybrid speciation between the two lineages.
Vitamin D3 and erythropoietin protect against renal ischemia-reperfusion injury via heat shock protein 70 and microRNA-21 expression
Authors: Golmohammadi Mohammad Ghasem , Banaei Shokofeh , Nejati Kazem , Chinifroush-Asl Mir Mehdi 
Published: December 2020

AbstractKidney ischemia reperfusion (IR) contributes to the development of acute kidney injury. The hypoxic conditions in ischemic damage lead to oxidative stress and apoptotic cell death. We investigated the effects of vitamin D3 (Vit D) and erythropoietin (EPO) on microRNA-21(miR-21) expression in renal IR. Wistar rats were divided into five groups including the control, vehicle + IR, Vit D + IR, EPO + IR, and Vit D + EPO + IR groups. The animals were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 h reperfusion. Vitamin D3 and EPO were administered prior to ischemia. After 24 h reperfusion, the kidney samples were collected for the detection of miR-21, heat shock protein 70 (hsp70) and caspase-3 expression levels. Kidney IR significantly increased the expression of miR-21, hsp70 and capase-3 and blood urea nitrogen (BUN)-Cr levels. Treatment with vitamin D3 and EPO significantly decreased the BUN-Cr levels and hsp70 and caspase-3 expression. Also, the co-administration of two drugs significantly increased miR-21 expression. It seems that vitamin D3 or EPO administration could protect the kidney against IR injury. However, vitamin D3 and EPO co-treatment was the most effective compared with the other treatment groups.
Clustering of CODEX clusters
Authors: Lindholm V. , Finoguenov A. , Comparat J. , Kirkpatrick C. C. , Rykoff E. , Clerc N. , Collins C. , Damsted S. , Ider Chitham J. , Padilla N. 
Published: December 2020

Context. The clustering of galaxy clusters links the spatial nonuniformity of dark matter halos to the growth of the primordial spectrum of perturbations. The amplitude of the clustering signal is widely used to estimate the halo mass of astrophysical objects. The advent of cluster mass calibrations enables using clustering in cosmological studies.
Aims. We analyze the autocorrelation function of a large contiguous sample of galaxy clusters, the Constrain Dark Energy with X-ray (CODEX) sample, in which we take particular care of cluster definition. These clusters were X-ray selected using the ROentgen SATellite All-Sky Survey and then identified as galaxy clusters using the code redMaPPer run on the photometry of the Sloan Digital Sky Survey. We develop methods for precisely accounting for the sample selection effects on the clustering and demonstrate their robustness using numerical simulations.
Methods. Using the clean CODEX sample, which was obtained by applying a redshift-dependent richness selection, we computed the two-point autocorrelation function of galaxy clusters in the 0.1 < z < 0.3 and 0.3 < z < 0.5 redshift bins. We compared the bias in the measured correlation function with values obtained in numerical simulations using a similar cluster mass range.
Results. By fitting a power law, we measured a correlation length r0 = 18.7 ± 1.1 and slope γ = 1.98 ± 0.14 for the correlation function in the full redshift range. By fixing the other cosmological parameters to their nine-year Wilkinson Microwave Anisotropy Probe values, we reproduced the observed shape of the correlation function under the following cosmological conditions: Ωm0 = 0.22−0.03+0.04 and S8 = σ8(Ωm0/0.3)0.5 = 0.85−0.08+0.10 with estimated additional systematic errors of σΩm0 = 0.02 and σS8 = 0.20. We illustrate the complementarity of clustering constraints by combining them with CODEX cosmological constraints based on the X-ray luminosity function, deriving Ωm0 = 0.25 ± 0.01 and σ8 = 0.81−0.02+0.01 with an estimated additional systematic error of σΩm0 = 0.07 and σσ8 = 0.04. The mass calibration and statistical quality of the mass tracers are the dominant source of uncertainty.
Possible approach to detecting the mysterious Saturnian convective dynamo through gravitational sounding
Authors: Liu Shanhong , Kong Dali , Yan Jianguo 
Published: December 2020

Context.Planetary dynamo research is mathematically and numerically difficult. Forward calculations are numerically expensive and subject to much uncertainty in key magnetohydrodynamics parameters. For a gaseous planet such as Saturn, even the precise location of its dynamo and typical convective strength are unknown, which further complicates studies.Aims.We test the idea of inversely probing Saturnian convective dynamo through gravitational sounding, based on the principle that the convective fluid motion can distort the internal density distribution and hence induce the gravitational anomaly.Methods.TheCassiniGrand Finale mission has reported unprecedentedly accurate measurements of the gravitational field of Saturn. An unexplained nonaxisymmetric component of the gravitational field was detected in the data. By performing precise orbit determination (POD) simulations, we studied the possibility that theCassinispacecraft might sense the dynamo-related nonaxisymmetric gravitational signature in the Grand Finale phase. In addition, further extensively simulated missions of various orbit configurations were carried out in order to explore promising mission strategies that might fulfill the objective of detecting the Saturnian convective dynamo.Results.Our POD simulations show that the gravity science carried out in theCassiniGrand Finale mission is insufficient to determine weak nonaxisymmetric gravitational moments because good subspacecraft-point coverage is lacking. The origin of the unexplained Saturnian gravity remains a puzzle. However, it is positively indicated by our simulations that future gravitational sounding is probably able to detect dynamo-related gravity when the subspacecraft-point coverage of a mission is sufficient. We suggest that the mission orbits be purposely designed into a near-polar orientation with a height of about 6000 km at periapsis and a moderate eccentricity of 0.5. A total POD tracking time of five months would enable the detection of the secular nonaxisymmetric gravitational moments that are caused by the deep convective dynamo of Saturn. The orbit strategy can facilitate engineering implementation by keeping the spacecraft marginally away from the Saturn radiation belt throughout the mission.
Study on the culture of Escherichia coli with different hydrolysis depth of globin
Authors: Song Zurong , Tao Ali , Xuehua Feng 
Published: December 2020

Objective: To explore the effect of culture medium made of peptide fragments of different sizes of globin hydrolyzed by protease on the culture of Escherichia coli.
Method: with fresh pig blood as raw material, hydrolyze pig blood protein under fixed conditions, and screen out the enzyme with the highest degree of hydrolysis. Then conduct orthogonal test on the enzyme to determine the best hydrolysis conditions; the beads were obtained under the best hydrolysis conditions. The Escherichia coli was cultured on different peptone culture bases that were prepared by hydrolysate going through different pore size ultrafiltration membrane samples. The culture of Escherichia coli at different hydrolysis depths is determined by detecting the total amount with Escherichia coli detection method.
Results: Trypsin has the best hydrolysis effect. The best hydrolysis conditions are as follows: enzyme dosage of 0.2%, hydrolysis time of 12h, temperature of 50°C, initial pH of 7.5 and mass fraction of substrate protein of 8%. Through growth curves of Escherichia coli, it is known that the smaller the pore size of the ultrafiltration membrane, the smaller the molecular weight of the peptide fragment, and the greater the density of the Escherichia coli bacterial solution.
Conclusion: Through analyzing the production curves of Escherichia coli in different culture medium, it is concluded that the smaller the molecular weight of the protein peptides, the higher the density of Escherichia coli solution in the LB liquid medium prepared by it, and the better the Escherichia coli culture effect.
Sexual Practices During Adolescence
Authors: Arruda Emanoela Priscila Toledo , Brito Luiz Gustavo Oliveira , Prandini Tatiana Rocha , Lerri Maria Rita , Reis Rosana Maria dos , Barcelos Thays Marina Roncato , Lara Lúcia Alves Silva 
Published: December 2020

AbstractAdolescence is characterized by significant biological and psychological changes. During this time, the increased production of androgens leads to increased sexual behavior, and this may contribute to early initiation of sexual activity. The objectives of the present cross-sectional study of adolescents enrolled in state schools in the city of Ribeirão Preto, state of São Paulo, Brazil, were to determine the average age at the first sexual intercourse (sexarche), the average number of sexual partners, and the frequency of contraceptive and condom use. Information on the age at sexarche, number of sexual partners, use of different contraceptive methods, and use of condoms were obtained using a semistructured questionnaire. Quantitative variables are expressed as means and standard deviations (SDs), and qualitative variables as absolute and relative frequencies. The chi-squared test was used for comparisons of qualitative variables, and the Student t-test for comparisons of continuous variables. All statistical analyses were performed using SAS (version 9.4, North Carolina State University, USA). We evaluated 202 students who answered the questionnaire, 69 males (36.36%) and 133 females (63.64%). The age at sexarche for men ranged from 7 to 18 years old, and for women from 7 to 17 years old. Forty-eight girls (36.01%) and 21 boys (30.43%) were in the first year of high school, 66.94% of adolescents reported sexual intercourse, and 56.25% used a condom during the first sexual intercourse. A total of 36.72% of students said they had safe sex most of the time, and 83.59% said that the first sexual intercourse happened because they “had a crush on” the other person.
Cytotoxic Activity of Antineoplastic Agents on Fertility: A Systematic Review
Authors: Moura Gabriel Acácio de , Monteiro Paula Bruno 
Published: December 2020

Abstract
Objective To analyze the long-term effects of antineoplastic treatments on patient fertility.
Selection of Studies The studies were selected through the New PubMed, Scielo and Lilacs databases along with references used for the creation of the present work. For the selection of studies, articles published between the periods from January 1, 2015 to April 6, 2020 in the English, Portuguese and Spanish languages were used. As inclusion criteria: cohort studies and studies conducted in vitro. As exclusion criteria: review articles, reported cases, studies that do not address thematic reproduction, studies that do not address the cancer theme, articles that used animals, articles that address the preservation of fertility and articles in duplicate in the bases.
Data Collection The collected data included: age of the patient at the beginning of treatment, type of neoplasm, type of antineoplastic treatment, chemotherapy used, radiotherapy dosage, radiotherapy site, effect of antineoplastic agents on fertility and number of patients in the study.
Data Synthesis Thirty studies were evaluated, antineoplastic chemotherapy agents and radiotherapy modulate serum hormone levels, reduces germ cell quantities and correlated with an increase in sterility rates. The effects mentioned occur in patients in the prepubertal and postpubertal age.
Conclusion Antineoplastic treatments have cytotoxic effects on the germ cells leading to hormonal modulation, and pubertal status does not interfere with the cytotoxic action of therapies.
Spectrum of F8 Genotype and Genetic Impact on Inhibitor Development in Patients with Hemophilia A from Multicenter Cohort Studies (J-HIS) in Japan
Authors: Shinozawa Keiko , Yada Koji , Kojima Tetsuhito , Nogami Keiji , Taki Masashi , Fukutake Katsuyuki , Yoshioka Akira , Shirahata Akira , Shima Midori ,   study group on JAPAN HEMOPHILIA INHIBITOR STUDY (J-HIS)
Published: December 2020

AbstractSome genetic and treatment-related factors are risk factors for inhibitor development in patients with hemophilia A (PwHA). However, the genotype distribution of the factor VIII gene (F8) and genetic impact on inhibitor development in Japanese PwHA remain unknown. In 2007, the Japan Hemophilia Inhibitor Study 2 (J-HIS2) was organized to establish a nationwide registry system for hemophiliacs and to elucidate risk factors for inhibitor development, designed for prospective investigation following a retrospective study (J-HIS1) which had already finished. Patients, newly diagnosed after January 2007, were enrolled in J-HIS2 and followed up for inhibitor development and clinical environments since 2008 onward. In the present study, F8 genotypes of PwHA were investigated in the patients recruited from the J-HIS2 cohort as well as those with inhibitor from the J-HIS1 cohort. F8 variants identified in 59 PwHA with inhibitor in J-HIS1 were: 20 intron-22 inversions, 5 intron-1 inversions, 9 large deletions, 4 nonsense, 8 missense, 11 small in/del, and 2 splice-site variants. F8 variants identified in 267 (67 with inhibitor) PwHA in J-HIS2 were: 76(28) intron–22 inversions, 3(2) intron–1 inversion, 1(0) duplication, 8(5) large deletions, 21(7) nonsense, 109(7) missense, 40(11) small in/del, and 9(7) splice-site variants. Forty variants were novel. The cumulative inhibitor incidence rate in the severe group with null changes was 42.4% (95% confidence interval [CI]: 33.7–50.8), higher than that with nonnull changes (15.6% [95%CI: 6.8–27.8]), in J-HIS2. Relative risk for inhibitor development of null changes was 2.89. The spectrum of F8 genotype and genetic impact on inhibitor development in Japanese PwHA were consistent with the previous reports.
Fruiting body form, not nutritional mode, is the major driver of diversification in mushroom-forming fungi
Authors: Sánchez-García Marisol , Ryberg Martin , Khan Faheema Kalsoom , Varga Torda , Nagy László G. , Hibbett David S. 
Published: December 2020

Significance
The Agaricomycetes is a conspicuous and successful group of Fungi, containing ∼36,000 described species. The group presents striking diversity in fruiting bodies, including those with a pileus (cap) and stipe (stalk), puffballs, coral fungi, crust-like forms, etc. Agaricomycetes also comprise ecologically diverse species, including decayers, mycorrhizal symbionts, and pathogens. We assembled a “megaphylogeny” with 8,400 species that represent ∼23% of the known diversity of Agaricomycetes and used it to investigate the relative impact of fruiting body forms and nutritional modes on diversification rates. Across all Agaricomycetes, a pileate-stipitate fruiting body is associated with increased diversification compared to other forms. No such relationship was found for nutritional modes, including mycorrhizal symbiosis. We conclude that morphological innovation has driven diversification in Agaricomycetes.
Does political settlements analysis capture the unsettling politics of oil in Africa?
Authors: Oppong Nelson 
Published: December 2020

SUMMARY
Analysis of political settlements has emerged from the shadows of new institutionalism and moved to the epicentre of political economy analysis across Africa. This debate takes on the framework by scrutinising its applicability to the politics of oil through the combined lens of critical political economy and contentious politics. It argues that contrary to the postulations of strategic elite bargains by political settlement researchers, Africa's oil landscape is marked by pluralistic politics and contestations at multiple scales.
Enhancement of luminescence and thermal stability in Sr2.92-xTmxSiO5:Eu2+ for white LEDs
Authors: Peng Lingling , Qinping Qiang , Cao Shixiu , Chen Wenbo , Han Tao 
Published: December 2020

Abstract
Sr2.92-xTmxSiO5:Eu2+ (x = 0–0.09) orange emitting phosphors for white LED were prepared by solid-state method. The crystal structure and morphology of Sr2.92-xTmxSiO5 were determined by XRD and SEM. The phosphor showed strong absorption spectra and can be excited by near-ultraviolet (NUV) light in the range of 200−550 nm which matched well with the NUV light-emitting diode (LED) chips. The emission spectra showed asymmetric band around 585 nm under NUV and assigned to the 4f65d-4f7 transition of Eu2+ occupied Sr2+ sites in the host lattice. It is observed that 5d electron state of Eu2+ which easily influenced by the crystal field was not changed after Tm3+ co-doping in the host. Enhanced emission intensity, improved thermostability and fluorencence decay time have been made via crystal chemical unequivalence substitution of Tm3+ in Sr2+ site. The energy transfer took place between the Tm3+ and Eu2+ activator, the underlying mechanisms of enhanced luminescence and decay fluorescence properties by energy transfer were analyzed.
Analysis of Bc
→ τντ
at CEPC *
Authors: Zheng Taifan , Xu Ji , Cao Lu , Yu Dan , Wang Wei , Prell Soeren , Cheung Yeuk-Kwan E. , Ruan Manqi 
Published: December 2020

Abstract
Precise determination of the

branching ratio provides an advantageous opportunity for understanding the electroweak structure of the Standard Model, measuring the CKM matrix element

, and probing new physics models. In this paper, we discuss the potential of measuring the process

with

decaying leptonically at the proposed Circular Electron Positron Collider (CEPC). We conclude that during the Z pole operation, the channel signal can achieve five-

significance with

Z decays, and the signal strength accuracies for

can reach around 1% level at the nominal CEPC Z pole statistics of one trillion Z decays, assuming the total

yield is

. Our theoretical analysis indicates the accuracy could provide a strong constraint on the general effective Hamiltonian for the

transition. If the total

yield can be determined to

(1%) level of accuracy in the future, these results also imply

could be measured up to

(1%) level of accuracy.
Intelligent detection system of asset security vulnerability hidden danger under multiple and heterogeneous Web network
Authors: Li Yonggang , Li Jie 
Published: December 2020

Abstract
—Traditional network security vulnerability detection system is affected by detection tools, and the detection accuracy of complex network vulnerability is low. Therefore, this paper designs an intelligent detection system of asset security vulnerabilities under the multi heterogeneous web network. By crawling the asset security data in web network by crawler, the crawling data is integrated by using the characteristics of multi heterogeneous network. According to different types of security vulnerabilities, vulnerability detection is carried out to realize the intelligent detection of network asset security vulnerabilities. In order to verify the detection effect of system security vulnerabilities, comparative experiments are designed. The results show that the average false alarm rate of the designed system is only 12.3%, which is nearly 40% lower than that of the traditional system, which effectively improves the vulnerability detection accuracy.
Construction of a neural network model for performance prediction in shot put athletes
Authors: Tuo Xinling , Li Tao 
Published: December 2020

Abstract
This paper is to investigate the role of artificial neural network models in the prediction of sports performance and to establish artificial neural network models to evaluate the correlation between athletes’ special performance and special physical quality. The results show that the artificial neural network model overcomes the shortcomings of multiple regression models and gray models that require a pre-determined mathematical model, and more accurately reflects the functional relationship between the training indexes of the special quality of shot put athletes and the special athletic performance, and more accurately predicts the special performance of shot put athletes. It further showed that the special physical quality of shot put athletes is an important basis for their special athletic ability. With the physical quality index as the independent variable and the special performance as the dependent variable, the prediction model of the athletes’ special performance can be established, which can accurately diagnose and evaluate the development level of the athletes’ physical quality, and clarify the key points and objectives of the training content, so as to improve the scientific level of the training of shot put.