bluuebunny/crossref_metadata_2025_split huggingface dataset:
A traffic light enzyme: acetate binding reversibly switches chlorite dismutase from a red- to a green-colored heme protein
Authors: Mahor Durga , Püschmann Julia , van den Haak Menno , Kooij Pepijn J. , van den Ouden David L. J. , Strampraad Marc J. F. , Srour Batoul , Hagedoorn Peter-Leon 
Published: April 2020

Abstract
Chlorite dismutase is a unique heme enzyme that catalyzes the conversion of chlorite to chloride and molecular oxygen. The enzyme is highly specific for chlorite but has been known to bind several anionic and neutral ligands to the heme iron. In a pH study, the enzyme changed color from red to green in acetate buffer pH 5.0. The cause of this color change was uncovered using UV–visible and EPR spectroscopy. Chlorite dismutase in the presence of acetate showed a change of the UV–visible spectrum: a redshift and hyperchromicity of the Soret band from 391 to 404 nm and a blueshift of the charge transfer band CT1 from 647 to 626 nm. Equilibrium binding titrations with acetate resulted in a dissociation constant of circa 20 mM at pH 5.0 and 5.8. EPR spectroscopy showed that the acetate bound form of the enzyme remained high spin S = 5/2, however with an apparent change of the rhombicity and line broadening of the spectrum. Mutagenesis of the proximal arginine Arg183 to alanine resulted in the loss of the ability to bind acetate. Acetate was discovered as a novel ligand to chlorite dismutase, with evidence of direct binding to the heme iron. The green color is caused by a blueshift of the CT1 band that is characteristic of the high spin ferric state of the enzyme. Any weak field ligand that binds directly to the heme center may show the red to green color change, as was indeed the case for fluoride.

Graphic abstract
Associations between road traffic noise exposure at home and school and ADHD in school-aged children: the TRAILS study
Authors: Zijlema W. L. , de Kluizenaar Y. , van Kamp I. , Hartman C. A. 
Published: April 2020

AbstractEnvironmental noise may play a role in the manifestation and severity of attention deficit/hyperactivity disorder (ADHD) symptoms, but evidence is limited. We investigated the cross-sectional associations between residential and school road traffic noise exposure and ADHD symptoms and diagnosis. The sample included n = 1710, 10–12-year-old children from the TRAILS study in The Netherlands. ADHD symptoms were measured using a DSM-IV based subscale from the Child Behavior Checklist. Children with diagnosed ADHD originated from the clinic-referred cohort. Road traffic noise (Lden) was estimated at the residence and school level, by model calculation. Risk ratios for ADHD symptoms and ADHD diagnoses, and regression coefficients for symptom severity were estimated separately and simultaneously for residential and school road traffic noise. Adjusted multinomial models with residential road traffic noise showed that residential noise was not associated with ADHD symptoms, but was associated with lower risks for ADHD diagnosis (RR = 0.93; 95% CI 0.89, 0.97). Similar associations were observed for models including school road traffic noise and models including both exposures. No clear exposure response relationship was observed for associations between residential or school noise and ADHD symptom severity. We found no evidence for a harmful association between road traffic noise and ADHD. Associations between noise and lower risks for ADHD were observed only in referred cases with a confirmed ADHD diagnosis and may be due to residual confounding or selection bias. Future studies should focus on residential and school noise exposure, and study associations with ADHD symptoms and diagnosis over time.
A randomized, phase 2 study of deoxyuridine triphosphatase inhibitor, TAS-114, in combination with S-1 versus S-1 alone in patients with advanced non-small-cell lung cancer
Authors: Yamamoto Nobuyuki , Hayashi Hidetoshi , Planchard David , Morán Teresa , Gregorc Vanesa , Dowell Jonathan , Sakai Hiroshi , Yoh Kiyotaka , Nishio Makoto , Cortot Alexis B. , Benhadji Karim A. , Soni Nital , Huang Jinhong , Makris Lukas , Cedres Susana 
Published: April 2020

SummaryIntroduction TAS-114 is a potent inhibitor of deoxyuridine triphosphatase, which is a gatekeeper protein preventing uracil and 5-fluorouracil (5-FU) misincorporation into DNA. TAS-114 has been suggested to enhance the antitumor activity of 5-FU. This randomized, phase 2 study investigated TAS-114 plus S-1 (TAS-114/S-1) vs. S-1 in non-small-cell lung cancer (NSCLC) patients. Methods Patients with advanced NSCLC, previously treated with ≥ 2 regimens, were randomized 1:1 to receive TAS-114 (400 mg)/S-1 (30 mg/m2) or S-1 (30 mg/m2). Progression-free survival (PFS, independent central review) was the primary endpoint. Secondary endpoints included disease control rate (DCR), overall survival (OS), overall response rate (ORR), and safety. Results In total, 127 patients received treatment. Median PFS was 3.65 and 4.17 months in the TAS-114/S-1 and S-1 groups, respectively (hazard ratio [HR] 1.16, 95% confidence interval [CI] 0.71–1.88; P = 0.2744). DCR was similar between groups (TAS-114/S-1 80.3%, S-1 75.9%) and median OS was 7.92 and 9.82 months for the TAS-114/S-1 and S-1 groups, respectively (HR 1.31, 95% CI 0.80–2.14; P = 0.1431). The ORR was higher in the TAS-114/S-1 group than the S-1 group (19.7% vs. 10.3%), and more patients with tumor shrinkage were observed in the TAS-114/S-1 group. Incidence rates of anemia, skin toxicities, and Grade ≥ 3 treatment-related adverse events were higher in the TAS-114/S-1 group compared with the monotherapy group. Conclusions Although the TAS-114/S-1 combination improved the response rate, this did not translate into improvements in PFS. Clinical Trial Registration No. NCT02855125 (ClinicalTrials.gov) registered on 4 August 2016.
Wavelet-based discrimination of isolated singularities masquerading as multifractals in detrended fluctuation analyses
Authors: Oświȩcimka Paweł , Drożdż Stanisław , Frasca Mattia , Gȩbarowski Robert , Yoshimura Natsue , Zunino Luciano , Minati Ludovico 
Published: April 2020

AbstractThe robustness of two widespread multifractal analysis methods, one based on detrended fluctuation analysis and one on wavelet leaders, is discussed in the context of time-series containing non-uniform structures with only isolated singularities. Signals generated by simulated and experimentally-realized chaos generators, together with synthetic data addressing particular aspects, are taken into consideration. The results reveal essential limitations affecting the ability of both methods to correctly infer the non-multifractal nature of signals devoid of a cascade-like hierarchy of singularities. Namely, signals harboring only isolated singularities are found to artefactually give rise to broad multifractal spectra, resembling those expected in the presence of a well-developed underlying multifractal structure. Hence, there is a real risk of incorrectly inferring multifractality due to isolated singularities. The careful consideration of local scaling properties and the distribution of Hölder exponent obtained, for example, through wavelet analysis, is indispensable for rigorously assessing the presence or absence of multifractality.
Automatic intraoperative optical coherence tomography positioning
Authors: Grimm Matthias , Roodaki Hessam , Eslami Abouzar , Navab Nassir 
Published: April 2020

Abstract
Purpose
Intraoperative optical coherence tomography (iOCT) was recently introduced as a new modality for ophthalmic surgeries. It provides real-time cross-sectional information at a very high resolution. However, properly positioning the scan location during surgery is cumbersome and time-consuming, as a surgeon needs both his hands for surgery. The goal of the present study is to present a method to automatically position an iOCT scan on an anatomy of interest in the context of anterior segment surgeries.

Methods
First, a voice recognition algorithm using a context-free grammar is used to obtain the desired pose from the surgeon. Then, the limbus circle is detected in the microscope image and the iOCT scan is placed accordingly in the X–Y plane. Next, an iOCT sweep in Z direction is conducted and the scan is placed to centre the topmost structure. Finally, the position is fine-tuned using semantic segmentation and a rule-based system.

Results
The logic to position the scan location on various anatomies was evaluated on ex vivo porcine eyes (10 eyes for corneal apex and 7 eyes for cornea, sclera and iris). The mean euclidean distances (± standard deviation) was 76.7 (± 59.2) pixels and 0.298 (± 0.229) mm. The mean execution time (± standard deviation) in seconds for the four anatomies was 15 (± 1.2). The scans have a size of 1024 by 1024 pixels. The method was implemented on a Carl Zeiss OPMI LUMERA 700 with RESCAN 700.

Conclusion
The present study introduces a method to fully automatically position an iOCT scanner. Providing the possibility of changing the OCT scan location via voice commands removes the burden of manual device manipulation from surgeons. This in turn allows them to keep their focus on the surgical task at hand and therefore increase the acceptance of iOCT in the operating room.
Tensile and compressive behaviour of additively manufactured AlSi10Mg samples
Authors: Sert Enes , Hitzler L. , Hafenstein S. , Merkel M. , Werner E. , Öchsner A. 
Published: April 2020

AbstractLaser powder-bed fusion has become one of the most important techniques in additive manufacturing. For guaranteeing the possibility of manufacturing highly specialized and advanced components, currently intensive research is carried out in this field. One area of this research is the material-specific macroscopic anisotropy, which is investigated in our work by comprehensive static mechanical experiments. The material which was tested within this study was the precipitation-hardenable AlSi10Mg alloy, with the focus on installation space orientation. Tensile and compression tests were performed, the results for the Young's modulus in compressive loading exceeded the previously known values of this material in tensile loading and achieved values of up to 79.8 GPa. As a result of this investigation, a chemical spectroscopic analysis was undertaken and from the actual chemical composition, a relative density of 99.86% of the samples was determined.
Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture
Authors: Zhang Xiao-Hua , He Xinxin , Austin Brian 
Published: April 2020

AbstractVibrio harveyi, which belongs to family Vibrionaceae of class Gammaproteobacteria, includes the species V. carchariae and V. trachuri as its junior synonyms. The organism is a well-recognized and serious bacterial pathogen of marine fish and invertebrates, including penaeid shrimp, in aquaculture. Diseased fish may exhibit a range of lesions, including eye lesions/blindness, gastro-enteritis, muscle necrosis, skin ulcers, and tail rot disease. In shrimp, V. harveyi is regarded as the etiological agent of luminous vibriosis in which affected animals glow in the dark. There is a second condition of shrimp known as Bolitas negricans where the digestive tract is filled with spheres of sloughed-off tissue. It is recognized that the pathogenicity mechanisms of V. harveyi may be different in fish and penaeid shrimp. In shrimp, the pathogenicity mechanisms involved the endotoxin lipopolysaccharide, and extracellular proteases, and interaction with bacteriophages. In fish, the pathogenicity mechanisms involved extracellular hemolysin (encoded by duplicate hemolysin genes), which was identified as a phospholipase B and could inactivate fish cells by apoptosis, via the caspase activation pathway. V. harveyi may enter the so-called viable but nonculturable (VBNC) state, and resuscitation of the VBNC cells may be an important reason for vibriosis outbreaks in aquaculture. Disease control measures center on dietary supplements (including probiotics), nonspecific immunostimulants, and vaccines and to a lesser extent antibiotics and other antimicrobial compounds.
Impact of Disasters on Community Medical Screening Examination and Vaccination Rates: The Case of the Sewol Ferry Disaster in Ansan, Korea
Authors: Kang EunKyo 
Published: April 2020

ABSTRACTObjective:In April 2014, the Sewol ferry sank off the coast of Korea, resulting in 304 deaths. Of these, 250 were local high school students from Ansan, and the disaster severely affected their community. This study investigated the association of this disaster with health examination, cancer screening rate, and vaccination rate.Methods:The study subjects were adults ages ≥19 years (11 026 Ansan residents and 1 361 624 non-residents of Ansan) who participated in the 2011–2016 Korea Community Health Survey. The national health screening program examination rate, cancer screening rate, and influenza vaccination rate in Ansan residents and non-residents were assessed and their responses compared using chi-square tests, multiple logistic regression analyses, and a stratification analysis according to depression.Results:After the disaster (2014–2016), non-residents received more health screening (adjusted odds ratio [aOR] = 1.13, P < 0.001), cancer screening (aOR = 1.41, P < 0.001), and vaccination (aOR = 1.10, P = 0.002) than Ansan residents. This difference was more evident in the group without a depressive mood.Conclusion:People living in disaster areas show lower rates of medical screening examination and receive fewer vaccinations after the disaster. To decrease health impacts by disaster, efforts to increase community health screenings and vaccinations may be needed.
Egonets as systematically biased windows on society
Authors: Feld Scott L. , McGail Alec 
Published: April 2020

AbstractA person’s egonet, the set of others with whom that person is connected, is a personal sample of society which especially influences that person’s experience and perceptions of society. We show that egonets systematically misrepresent the general population because each person is included in as many egonets as that person has “friends.” Previous research has recognized that this unequal weighting in egonets leads many people to find that their friends have more friends than they themselves have. This paper builds upon that research to show that people’s egonets provide them with systematically biased samples of the population more generally. We discuss how this ubiquitous egonet bias may have far reaching implications for people’s experiences and perceptions of frequencies of other people’s ties and traits in ways that may influence their own feelings and behaviors. In particular, these egonet biases may help explain people’s tendencies to disproportionately experience and overestimate the prevalence of certain types of deviance and other social behaviors and consequently be influenced toward them. We illustrate egonet bias with analyses of all friends among 63,731 Facebook users. We call for further empirical investigation of egonet biases and their consequences for individuals and society.
AN UPDATED HISTORY OF PRE-CONTACT NEW ENGLAND: NEW AMS DATES FOR THE HORNBLOWER II AND FRISBY-BUTLER ARCHAEOLOGICAL SITES
Authors: Watson Jessica E 
Published: April 2020

ABSTRACTFaunal assemblages from the pre-Contact period sites Frisby-Butler and Hornblower II on Marthaʼs Vineyard, Massachusetts, USA, remain unstudied since excavation during the 1980s. This project establishes radiocarbon (14C) dates from faunal remains and evaluates occupation and abandonment at each site. 14C measurements were collected from 17 specimens and 13 dates from previous analyses were re-examined. Dates were identified from the archaeological time periods Transitional Archaic (2700–3700 BP), Early Woodland (2000–2700 BP), and Late Woodland (450–1200 BP) at Frisby-Butler. Occupation likely represented seasonal visitations during autumn and winter to hunt based on white-tailed deer (Odocoileus virginianus) demographic profiles. A combined dataset of new and re-calibrated 14C measurements from Hornblower II date to the Late Archaic (3700–6000 BP), Early Woodland, Middle Woodland (1200–2000 BP), and Late Woodland periods. Settlement was focused on gathering warm-weather foods like demersal fish and lakebirds. Together, the sites demonstrate periodic seasonal use of the southwest coast of the island throughout the Late Holocene and fit within an established regional pattern in southern New England.
Translating the promise of 5HT4 receptor agonists for the treatment of depression
Authors: Murphy Susannah E , de Cates Angharad N , Gillespie Amy L , Godlewska Beata R , Scaife Jessica C , Wright Lucy C , Cowen Philip J , Harmer Catherine J 
Published: April 2020

AbstractAnimal experimental studies suggest that 5-HT4 receptor activation holds promise as a novel target for the treatment of depression and cognitive impairment. 5-HT4 receptors are post-synaptic receptors that are located in striatal and limbic areas known to be involved in cognition and mood. Consistent with this, 5-HT4 receptor agonists produce rapid antidepressant effects in a number of animal models of depression, and pro-cognitive effects in tasks of learning and memory. These effects are accompanied by molecular changes, such as the increased expression of neuroplasticity-related proteins that are typical of clinically useful antidepressant drugs. Intriguingly, these antidepressant-like effects have a fast onset of their action, raising the possibility that 5-HT4 receptor agonists may be a particularly useful augmentation strategy in the early stages of SSRI treatment. Until recently, the translation of these effects to humans has been challenging. Here, we review the evidence from animal studies that the 5-HT4 receptor is a promising target for the treatment of depression and cognitive disorders, and outline a potential pathway for the efficient and cost-effective translation of these effects into humans and, ultimately, to the clinic.
Ambiguity and Conflict in Policy Implementation: The Case of the New Care Models (Vanguard) Programme in England
Authors: COLEMAN ANNA , BILLINGS JENNY , ALLEN PAULINE , MIKELYTE RASA , CROKE SARAH , MACINNES JULIE , CHECKLAND KATH 
Published: April 2020

AbstractPolicy driven change is challenging, with a significant gap between theory and practice. A key tension in enacting such change is achieving a balance between bottom-up development of local, context-specific approaches, and top-down, centrally determined policy solutions and their mutual sequencing. Ideal type models of the policy-making process envisage a rational ordered approach, driven by evidence and accompanied by ongoing evaluation of outcomes (Parsons, 1995, p77); however, the reality is far more complex. We examine the implementation and early operation of the New Care Models (NCM) Vanguard programme in England, using Matland’s (1995) ambiguity-conflict model, to explore the aims and expectations of the programme. We consider the relationship between top-down and bottom-up approaches to policy development and draw attention to the pressures coming from what was initially perceived as a permissive policy approach of encouraging experimentation, whilst also requiring rapid learning, scale and spread. We suggest that future programmes for large-scale policy implementation initiatives could be crafted differently to take account of the environment of implementation and render ambitions more realistic. Rather than aiming to create a set of definite products and templates, it may be that a set of principles for design and implementation should be developed and spread.
The challenges and considerations of community-based preparedness at the onset of COVID-19 outbreak in Iran, 2020
Authors: Moradzadeh Rahmatollah 
Published: April 2020

Abstract
COVID-19 as an emerging disease has spread to 183 countries and territories worldwide as of 20 March 2020. The first COVID-19 case (i.e. the index case) in Iran was observed in the city of Qom on 19 February 2020. One of the cities of Markazi Province is Delijan, which shares a border with Qom. Consequently, COVID-19 has quickly spread in this city because a large population commutes daily between the two cities. This study aimed to report the challenges and considerations of community-based preparedness at the onset of COVID-19 outbreak in a city of Iran in 2020.
SMT-based verification of data-aware processes: a model-theoretic approach
Authors: Calvanese Diego , Ghilardi Silvio , Gianola Alessandro , Montali Marco , Rivkin Andrey 
Published: April 2020

AbstractIn recent times, satisfiability modulo theories (SMT) techniques gained increasing attention and obtained remarkable success in model-checking infinite-state systems. Still, we believe that whenever more expressivity is needed in order to specify the systems to be verified, more and more support is needed from mathematical logic and model theory. This is the case of the applications considered in this paper: we study verification over a general model of relational, data-aware processes, to assess (parameterized) safety properties irrespectively of the initial database (DB) instance. Toward this goal, we take inspiration from array-based systems and tackle safety algorithmically via backward reachability. To enable the adoption of this technique in our rich setting, we make use of the model-theoretic machinery of model completion, which surprisingly turns out to be an effective tool for verification of relational systems and represents the main original contribution of this paper. In this way, we pursue a twofold purpose. On the one hand, we isolate three notable classes for which backward reachability terminates, in turn witnessing decidability. Two of such classes relate our approach to conditions singled out in the literature, whereas the third one is genuinely novel. On the other hand, we are able to exploit SMT technology in implementations, building on the well-known MCMT (Model Checker Modulo Theories) model checker for array-based systems and extending it to make all our foundational results fully operational. All in all, the present contribution is deeply rooted in the long-standing tradition of the application of model theory in computer science. In particular, this paper applies these ideas in an original mathematical context and shows how these techniques can be used for the first time to empower algorithmic techniques for the verification of infinite-state systems based on arrays, so as to make such techniques applicable to the timely, challenging settings of data-aware processes.
Stem cell-derived polarized hepatocytes
Authors: Dao Thi Viet Loan , Wu Xianfang , Belote Rachel L. , Andreo Ursula , Takacs Constantin N. , Fernandez Joseph P. , Vale-Silva Luis Andre , Prallet Sarah , Decker Charlotte C. , Fu Rebecca M. , Qu Bingqian , Uryu Kunihiro , Molina Henrik , Saeed Mohsan , Steinmann Eike , Urban Stephan , Singaraja Roshni R. , Schneider William M. , Simon Sanford M. , Rice Charles M. 
Published: April 2020

AbstractHuman stem cell-derived hepatocyte-like cells (HLCs) offer an attractive platform to study liver biology. Despite their numerous advantages, HLCs lack critical in vivo characteristics, including cell polarity. Here, we report a stem cell differentiation protocol that uses transwell filters to generate columnar polarized HLCs with clearly defined basolateral and apical membranes separated by tight junctions. We show that polarized HLCs secrete cargo directionally: Albumin, urea, and lipoproteins are secreted basolaterally, whereas bile acids are secreted apically. Further, we show that enterically transmitted hepatitis E virus (HEV) progeny particles are secreted basolaterally as quasi-enveloped particles and apically as naked virions, recapitulating essential steps of the natural infectious cycle in vivo. We also provide proof-of-concept that polarized HLCs can be used for pharmacokinetic and drug-drug interaction studies. This novel system provides a powerful tool to study hepatocyte biology, disease mechanisms, genetic variation, and drug metabolism in a more physiologically relevant setting.