bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic investigation of atypical malaria cases in Kanel, northern Senegal
Authors: Sy Mouhamad , Badiane Aida Sadikh , Siddle Katherine J. , Gaye Amy , Ndiaye Tolla , Deme Awa Bineta , Dieye Baba , Ndiaye Yaye Die , Diallo Mamadou Alpha , Diongue Khadim , Seck Mame Cheikh , Ndiaye Ibrahima Mbaye , Fall Fatou Ba , Cissé Moustapha , Gueye Alioune Badara , Sène Doudou , Dieye Yakou , Souané Tamba , MacInnis Bronwyn , Volkman Sarah K. , Wirth Dyann F , Ndiaye Daouda 
Published: August 2020

Abstract
BackgroundDiagnosis of malaria cases in regions where the malaria burden has decreased significantly and prevalence is very low becomes more challenging, in part because of reduced clinical presumption of malaria. The appearance of a cluster of malaria cases with atypical symptoms in Mbounguiel, a village in northern Senegal where malaria transmission is low, in September 2018 exemplifies this scenario. The collaboration between the national malaria control program (NMCP) at the Senegal ministry of health and the laboratory of parasitology mycology at Cheikh Anta Diop University worked together to evaluate this cluster of malaria cases using molecular and serology tools.ResultsNine of the fifteen patients were evaluated, and all nine samples were found to be positive for P. falciparum only. The 24 SNP molecular barcode showed the predominance of polygenomic infections, with identifiable strains being different from one another. All patients tested positive for the P. falciparum antigens. No other pathogenic infection was detected by either the serological panel or metagenomic sequencing. ConclusionsThis work undertaken locally within Senegal as a collaboration between the NMCP and a research malaria laboratory at University of Cheikh Anta Diop (UCAD) revealed that a cluster of malaria cases were caused by different strains of P. falciparum. The public health response in real time demonstrates the value of local molecular and genomics capacity in affected countries disease control and elimination.
Two-dimensional ferroelectric channel transistors integrating ultra-fast memory and neural computing
Authors: Wang Shuiyuan , Liu Lan , Gan Lurong , chen Huawei , Hou Xiang , Ding Yi , Ma Shunli , Zhang Wei , Zhou Peng 
Published: August 2020

Abstract
With the advent of big data era, applications are more data-centric, and energy efficiency issues caused by frequent data interactions due to the physical separation of memory and computing will become more severe. Emerging technologies have been proposed to perform analog computing with memory to address the dilemma. Ferroelectric memory has become a promising technology due to field-driven fast switching and non-destructive readout, but endurance and miniaturization are limited. Here, we demonstrate the α-In2Se3 ferroelectric semiconductor channel device that integrates non-volatile memory (NVM) and neural computation functions. Remarkable performance includes NVM ultra-fast write speed of 40ns, improved endurance through internal electric field, flexible adjustment of neural plasticity, energy consumption as low as 40fJ per event, and thermally modulated 94.74% high-precision iris recognition classification simulation. This prototypical demonstration laid the foundation for an integrated memory computing system with high density and energy efficiency.
Influence of MIF Polymorphisms on CpG Island Hyper-methylation of CDKN2A in the Patients With Ulcerative Colitis
Authors: Sakurai Naoko , Shibata Tomoyuki , Nakamura Masakatsu , Takano Hikaru , Hayashi Tasuku , Ota Masafumi , Nomura-Horita Tomoe , Hayashi Ranji , Shimasaki Takeo , Otsuka Toshimi , Tahara Tomomitsu , Arisawa Tomiyasu 
Published: August 2020

Abstract
Background: CDKN2A hypermethylation is among of the major events associated with carcinogenesis and is also observed in non-neoplastic colonic mucosa in patients with ulcerative colitis (UC). Macrophage migration inhibitory factor (MIF) plays a crucial role in promoting gastrointestinal inflammation characteristic of UC. The aim of this study is to explore associations between CDKN2A methylation status and MIF polymorphisms (rs755622 and rs5844572). Methods: One hundred and fifty-nine patients diagnosed with UC were enrolled in this study. The methylation status of p14ARF and p16INK4a was determined by MSP; MIF genotypes were identified by PCR-SSCP. Results: The frequencies of the rs755662 GC + CC genotype and rs5844572 7-repeat allele were significantly higher in group with methylated p14ARF and methylated p16INK4a, respectively. Carrying the rs755622 C allele indicated a significantly higher risk for p14ARF methylation (odds ratio (OR), 2.16; 95% confidence interval (CI), 1.08-4.32); similarly, carrying the rs5844572 7-repeat allele indicated a significantly higher risk for p16INK4a methylation (OR, 2.57; 95% CI, 1.26–5.24) after an adjusted regression analysis. The carriers of the rs755662 C allele and the rs5844572 7-repeat allele were both at a significantly higher risk for methylation of both p14ARF and p16INK4a when compared to the cohort in which neither of the genes were methylated (OR, 2.70; 95% CI, 1.22–6.01 and OR, 2.87; 95% CI, 1.25–6.62, respectively). Conclusions: Taken together, our findings suggest that MIF genotypes associated with inflammation may also be involved in promoting carcinogenesis via CDKN2A hyper-methylation in patients diagnosed with UC.
Peripheral nerve activity potential difference among TaiYang, TaiYin, ShaoYang and ShaoYin
Authors: JIN Li-yan , JIANG Jin-long , ZHENG Dao-xuan , JIN Chunyu 
Published: August 2020

Abstract
Objects

Explain the mechanism of PNAP Characteristic changes for each Korean Medicine Sasang typology.
Methods

By ways of question form and expert’s deliberation, 1000 healthy volunteers are distinguished into corresponding Sasang types, then detect median, ulnar, peroneal, tibial nerves’ CMAP and F-Wave, to analyze nerve conduction’s characteristic changes between groups or within group.
Results

① Within group, TaiYin: Median nerve’s right side MCV is declined than left side; Ulnar and Peroneal and tibial nerve’s left side MCV is declined than right side. ShaoYang: Ulnar and tibial nerve’s left side MCV is declined than right side. ( P < 0.05 ). ② Between groups, TaiYin & ShaoYang: About median and tibial nerve’s Distal Amlitude ( DA ), MCV, TaiYin is declined than that of ShaoYang; TaiYin & ShaoYin: About tibial nerve’s DA, MCV, The shortest latency of F-wave ( FL ), TaiYin is declined or extended than that of ShaoYin; ShaoYang & ShaoYin: For median nerve’s MCV, FL, ShaoYin is declined or extended than that of ShaoYang ( P < 0.05 ). ③ Nerve amount rate of FL ≥ 2SD ( ShaoYang as standard), for peroneal and tibial nerves, TaiYin is greater than ShaoYang and ShaoYin ( P < 0.01). ④ Nerve amount rate which conduction is under-activity, for tibial nerve, TaiYin is greater than ShaoYin ( right ) and ShaoYang ( left ) ( P < 0.01). ⑤ AMP. / DUR. rate of median nerve: ShaoYin > ShaoYang > TaiYin ( P < 0.05 ).
Conclusions

Median nerve’s left side has advantage than right side, however the ulnar and tibial nerve’s right side is well than left side, this characteristic is much more obvious in TaiYin; PNAP of ShaoYang is well than TaiYin and ShaoYin; AMP. / DUR. rate of median nerve shows sensitively about constitutional differences.
Significance

Provide foundation of clinical diagnosis and treatment on a constitutional basis.
Assessing a national policy on strengthening chronic care in primary care settings of a low-resource country using patients' perspectives.
Authors: Suriyawongpaisal Paibul , Aekplakorn Wichai , Srithamrongsaw Samrit , Kaewkamjonchai Phanuwich 
Published: August 2020

Abstract
Background To improve care for patients with chronic diseases, a recent policy initiative in Thailand focuses on strengthening primary care including training of the team to deliver healthcare based on the concept of Chronic Care Model(CCM). This study conducted a cross-sectional survey of 4,071 patients with hypertension and/or diabetes registered to 25 primary care units and 16 hospital NCD clinics in 11 provinces (76 in total) to examine the effects of provider training and local health systems settings on patients’ perception of the chronic care quality.Methods A home-based interview with questionnaire was conducted on the patients in primary care settings. The questionnaire was adopted from the Thai version of the Patient Assessment of Chronic Illness Care (PACIC+) developed by the MacColl Institute for Healthcare Innovation. The questionnaire contains 20 items from the original PACIC, which measure different parts of the CCM, and an additional 6 items assess the 5A Model.Mixed effect models were employed to compare subscale of patient perception of the care quality between trained upgraded PCUs, upgraded PCUs, ordinary PCUs and NCD clinics. Upgraded PCUs were ordinary PCUs with the multiprofessional team including a physician. Trained upgraded PCUs were upgraded PCUs with the training input.Results Mixed effect models depicted an independent association between every PACIC subscale (as a measure of CCM) and facility type with the maximum likelihood for patients of ordinary PCU reporting high to highest scores (ORs: 1.52-1.76; p<0.05) compared to hospital NCD clinics. This is also the case for patients: seeing the same doctor on repeated visits (ORs: 1.66-1.87; p<0.05) or having phone contacts of the providers (ORs:1.42-1.63; p<0.05). Similarly, across all of the 5A model subscales, ORs for patients attending ordinary PCU responded with high to highest scores were 1.4-2.0 times compared to those for patients attending hospital NCD clinics(p<0.05). Conclusions We could not find evidence to support effectiveness of the training approach. The training failure might indicate a need to address mismatch between health workforce and workload. It also indicates a need to incorporate fidelity check into any training program for chronic care addressing the complex healthcare needs.
Transplantation of a Heart Donated After Circulatory Death via Thoraco-Abdominal Normothermic Regional Perfusion. Results from the first Spanish case.
Authors: Redondo Marina Pérez , Carmona Sara Alcántara , García Susana Villar , Gil Alberto Forteza , Fernández Hector Villanueva , Hernández-Pérez Francisco José , Cruz Jose Luis Campo-Cañaveral de la , Calvo Rocío Velasco , Segovia-Cubero Javier , Menárguez Beatriz Alonso , Gallegos Francisco del Río , Coll Elisabeth , Gonzalez Beatriz Domínguez-Gil , Muñoz Juan José Rubio 
Published: August 2020

Abstract
BackgroundControlled donation after circulatory death (cDCD) has emerged as one of the main strategies for increasing the organ donor pool. Because of the ischemic injury that follows the withdrawal of life-sustaining therapies, hearts from cDCD donors have not been considered for transplantation until recently. The ex-situ perfusion of hearts directly procured from cDCD donors has been used to allow the continuous perfusion of the organ and the assessment of myocardial viability prior to transplantation.Based on our experience with abdominal normothermic regional perfusion in cDCD, we designed a protocol to recover and validate hearts from cDCD donors using thoraco-abdominal normothermic regional perfusion without any ex-situ device.Case presentationWe describe the first case of a cDCD heart transplant performed with this approach in Spain. The donor was a 43-year-old asthmatic female diagnosed with severe hypoxic encephalopathy. She was considered the potential cDCD donor as a suitable candidate for multiorgan procurement including the heart via thoraco-abdominal normothermic regional perfusion. The heart recipient was a 60-year-old male diagnosed with amyloid cardiomyopathy. Cold ischemia time was 55 minutes. The surgery was uneventful.ConclusionsThis case report has revealed the feasibility of evaluating and successfully transplanting cDCD hearts without the need for ex-situ perfusion based on the use of thoraco-abdominal normothermic regional perfusion. It opens up the way for multiorgan donation in cDCD.
Biofilm prevention potential of 0.02% polyhexanide irrigation solution in urethral catheters under practice-like in vitro conditions
Authors: Brill Florian H. H. , Hambach Julia , Utpatel Christian , Mogrovejo Diana , Gabriel Henrik , Klock Jan-Hendrik , Steinmann Joerg , Arndt Andreas 
Published: August 2020

Abstract
Background
Long-term use of indwelling urethral catheters is associated with high risk of urinary tract infection (UTI) and blockage. Microbial biofilms are a common cause of catheter blockage, reduce their lifetime and significantly increase morbidity of UTIs. A 0.02% polyhexanide irrigation solution developed for routine mechanical rinsing shows potential for bacterial decolonization of suprapubic and indwelling urethral catheters and has the potential to reduce or prevent biofilm formation.
Methods
Using a practice-like in vitro assay and standard silicon catheters, artificially contaminated with clinically relevant bacteria, assays were carried out to evaluate the biofilm reduction and prevention potential of polyhexanide vs. no intervention (standard approach) and irrigation with saline solution (NaCl 0.9%). The biofilm mass was measured by crystal violet staining and fluorescence microscopy.
Results
Irrigation with a 0.02% polyhexanide solution reduced the biofilm mass by approx. 85% vs. non-treated catheters. Standard 0.9% saline solution was able to reduce the biofilm mass by approx. 50%. Fluorescence microscopy showed that polyhexanide is able to destroy bacteria in the biofilm, albeit only those cells on the upper layers.
Conclusions
The polyhexanide and standard saline solutions are able to reduce bacterial biofilm from urinary catheters, showing a combination of mechanical and antibacterial effects. The data supports a prevention strategy to avoid the formation of a thick biofilm, which is characteristically difficult to be efficiently removed. Further research is required to evaluate the long-term tolerability and efficacy of polyhexanide in clinical practice.
Improvement of Renal Function After Transcatheter Aortic Valve Replacement and Its Impact on Survival
Authors: Frank Derk , Kylies Dominik , Freitag-Wolf Sandra , Fulisch Florian , Seoudy Hatim , Kuhn Christian , Kihm Lars Philipp , Pühler Thomas , Lutter Georg , Dempfle Astrid , Frey Norbert , Feldkamp Thorsten 
Published: August 2020

Abstract
BackgroundChronic kidney disease as well as acute kidney injury are associated with adverse outcomes after transcatheter aortic valve replacement (TAVR). However, little is known about the prognostic implications of an improvement in renal function after TAVR. Methods Renal improvement (RI) was defined as a decrease in postprocedural creatinine in µmol/l of ≥ 1% compared to its preprocedural baseline value. A propensity score representing the likelihood of RI was calculated to define patient groups which were comparable regarding potential confounders (age, sex, BMI, NYHA classification, STS score, log. EuroSCORE, history of atrial fibrillation/atrial flutter, pulmonary disease, previous stroke, CRP, creatinine, hsTNT and NT-proBNP). The cohort was stratified into 5 quintiles according to this propensity score and the survival time after TAVR was compared within each subgroup. Results Patients in quintile 5 (n=93) had the highest likelihood for RI. They were characterized by higher creatinine, lower eGFR, higher NYHA class, higher NT-proBNP, being mostly female and having shorter overall survival time. Within quintile 5, patients without RI had significantly shorter survival compared to patients with RI (p=0.002). There was no survival time difference between patients with and without RI in the whole cohort (p=0.12) and in quintiles 1 to 4 (all p > 0.16).Conclusions We here describe a propensity score-derived specific subgroup of patients in which RI after TAVR correlated with a significant survival benefit.
Evaluation of optimal fermentation conditions for volatile fatty acids production from artisanal fish waste
Authors: Cadavid-Rodríguez Luz Stella , Castro-López Viviana E. , PLACIDO JERSSON 
Published: August 2020

Abstract
In this research, operational parameters were assessed to optimise the production of volatile fatty acids (VFA) from artisanal fish waste (FW) using mixed-culture fermentation. The experiments were performed using a randomised block design with pH and Inoculum/Substrate (I/S) ratio as factors with three pH levels (5, 7 and 9) and five I/S ratios levels (0.20, 0.15, 0.10, 0.05, 0.00) with VFA production, total ammonia nitrogen (TAN), biogas and soluble chemical oxygen demand (sCOD) as response variables. The results indicated that pH and I/S ratio significantly influenced VFA production and the other variables evaluated. The highest VFA concentration (54.5 g/L) was obtained at pH 7 and with an I/S ratio of 0.05. These conditions were also optimal for TAN and sCOD. External pH control was proved as unnecessary as the highest VFA production (70.3 g VFA/L) was obtained without pH control. In these conditions, the fermentation broth was able to maintain its pH between 6.6 and 7.7. The VFA profile had acetic (35%) and butyric (32%) acids as the dominant species, however, propionic, iso-valeric and iso-butyric were also produced in small amounts (< 10%). These results evidence the potential of artisanal FW as a raw material for producing high concentrations of VFA and smooth the path for the establishment of a VFA and biogas biorefinery from artisanal FW.
IL-17A Polymorphism (rs2275913) and Levels Are Associated With Preeclampsia Pathogenesis in Chinese Patients&nbsp;&nbsp;
Authors: Lang Xiao , Liu Wei , Hou Yanyan , Zhao Wenxia , Yang Xingyu , Chen Lan , Yan Qi , Cheng Weiwei 
Published: August 2020

Abstract
BackgroundPreeclampsia (PE) is a pregnant related syndrome affecting both child and mother. Although, the role of various inflammatory molecules in PE has been demonstrated, importance of proinflammatory molecules such as IL-17A, IL-23 are poorly understood. In the present investigation, we tested a possible association of common genetic variants in IL-17A and IL-23 gene with PE. MethodsClinically diagnosed 115 PE patients who reported to the International Peace Maternity and Child Health Hospital were enrolled in the present study. Age, sex-matched 102 pregnant women, and 147 healthy Chinese women were also included. ELISA was used to quantify serum levels of IL-17A and IL-23 in all enrolled subjects. Common genetic polymorphisms in IL-17A (rs 2275913, rs1974226, and rs1974226), IL-23A (rs11171806), and IL-12B (rs3212227) were genotyped by PCR-RFLP or TaqMan probe-based method. ResultsElevated levels of serum IL-17A was observed in PE patients compared to pregnant (P<0.0001) and healthy women (P<0.0001). However, IL-23 levels were comparable among different clinical categories. Furthermore, heterozygous (GA) and minor allele (A) for IL-17A (rs2275913) and IL-23A (rs11171806) were more prevalent in PE patients in comparison to pregnant ladies or healthy women suggesting an essential role in predisposition to PE development. Interestingly, IL-17A (rs 2275913) mutants were linked with elevated IL-17A levels in comparison to wildtype (GG). ConclusionsIL-17A (rs2275913) variants are associated with higher serum levels of cytokine, and predisposed PE development.
Multiple waves of freshwater colonization of the three-spined stickleback in the Japanese Archipelago
Authors: Kakioka Ryo , Mori Seiichi , Kokita Tomoyuki , Hosoki Takuya K , Nagano Atsushi J , Ishikawa Asano , Kume Manabu , Toyoda Atsushi , Kitano Jun 
Published: August 2020

Abstract
Background: The three-spined stickleback (Gasterosteus aculeatus) is a remarkable system to study the genetic mechanisms underlying parallel evolution during the transition from marine to freshwater habitats. Although the majority of previous studies on the parallel evolution of sticklebacks have mainly focused on postglacial freshwater populations in the Pacific Northwest of North America and northern Europe, we recently use Japanese stickleback populations for investigating shared and unique features of adaptation and speciation between geographically distant populations. However, we currently lack a comprehensive phylogeny of the Japanese three-spined sticklebacks, despite the fact that a good phylogeny is essential for any evolutionary and ecological studies. Here, we conducted a phylogenomic analysis of the three-spined stickleback in the Japanese Archipelago.Results: We found that freshwater colonization occurred in multiple waves, each of which may reflect different interglacial isolations. Some of the oldest freshwater populations from the central regions of the mainland of Japan (Hariyo populations) were estimated to colonize freshwater approximately 170,000 years ago. The next wave of colonization likely occurred approximately 100,000 years ago. The inferred origins of several human-introduced populations showed that introduction occurred mainly from nearby habitats. We also found a new habitat of the three-spined stickleback sympatric with the Japan Sea stickleback (Gasterosteus nipponicus).Conclusions: These Japanese stickleback systems differ from those in the Pacific Northwest of North America and northern Europe in terms of divergence time and history. Stickleback populations in the Japanese Archipelago offer valuable opportunities to study diverse evolutionary processes in historical and contemporary timescales.
Bariatric surgery can acutely modulate ER-stress and inflammation on subcutaneous adipose tissue in non-diabetic patients with obesity
Authors: Ferraz-Bannitz Rafael , Welendorf Caroline Rossi , Coelho Priscila Oliveira , Salgado Wilson , Nonino Carla Barbosa , Beraldo Rebeca , Foss-Freitas Maria 
Published: August 2020

Abstract
Background
Bariatric surgery, especially Roux-en-Y gastric bypass (RYGB) is the most effective and durable treatment option for population with severe obesity. The mechanisms involving adipose tissue may be important to explain the effects of surgery.
Methods
We aimed to identify the genetic signatures of adipose tissue in patients undergoing RYGB. We evaluated 13 obese, non-diabetic patients (mean age 37 years, 100% women, Body mass index (BMI) 42.2 kg/m2) one day before surgery, 3 and 6 months (M) after RYGB.
Results
Analysis of gene expression in adipose tissue collected at surgery compared with samples collected at 3M and 6M Post-RYGB showed that interleukins (Interleukin 6, Tumor necrosis factor-α (TNF-α), and Monocyte chemoattractant protein-1(MCP1)) and endoplasmic reticulum stress (ERS) genes (Eukaryotic translation initiation factor 2 alpha kinase 3 (EIF2AK3) and Calreticulin (CALR)) decreased during the follow-up (P ≤ 0.01 for all). Otherwise, genes involved in energy homeostasis (Adiponectin and AMP-activated protein kinase (AMPK)), cellular response to oxidative stress (Sirtuin 1, Sirtuin 3, and Nuclear factor erythroid 2-related factor 2 (NRF2)), mitochondrial biogenesis (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α)) and amino acids metabolism (General control nonderepressible 2 (GCN2)) increased from baseline to all other time points evaluated (P ≤ 0.01 for all). Also, expression of Peroxisome proliferator activated receptor gamma (PPARϒ) (adipogenesis regulation) was significantly decreased after RYGB (P < 0.05) We also observed a strong positive correlation between PGC1α, SIRT1 and AMPK with BMI at 3M (P ≤ 0.01 for all) and ADIPOQ and SIRT1 with BMI at 6M (P ≤ 0.01 for all).
Conclusions
Our findings demonstrate that weight loss is associated with amelioration of inflammation and ERS and increased protection against oxidative stress in adipose tissue. These observations are strongly correlated with a decrease in BMI and essential genes that control cellular energy homeostasis, suggesting an adaptive process on a gene expression level during the caloric restriction and weight loss period after RYGB.