bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-Wide Identification and Characterization of GATA Family Genes in Brassica Napus
Authors: Zhu Weizhuo , Guo Yiyi , Chen Yeke , Wu Dezhi , Jiang Lixi 
Published: June 2020

Abstract
Background: GATA transcription factors are involved in plant developmental processes, which also respond to environmental stresses through binding DNA regulatory regions to regulate the downstream genes. However, little information of GATA genes is available in Brassica napus, one of important oil crops. The release of B. napus genome sequences provides a good opportunity to perform a genome-wide characterization of GATA family genes in the rapeseed genome. Results: In this study, 96 GATA genes randomly distributing on 19 chromosomes were identified in B. napus. They were classified into four subfamilies on the basis of phylogenetic analysis and the domain structures. The sequences of BnGATAs were obvious divergence among four subfamilies based on their GATA domains, structures and motif compositions. Gene duplication and the synteny between the genomes of B. napus and A. thaliana were also analyzed to provide insights into the evolutionary characteristics. Moreover, BnGATAs showed different expression patterns in various tissues and under diverse abiotic stresses. Single nucleotide polymorphisms (SNPs) distribution analysis suggests functional disparity of GATA family genes in different genotypes of a core collection germplasm.Conclusion: This study examined genomic structures, evolution features, expression patterns and SNP distribution of 96 BnGATAs. The results enriched our knowledge of the GATA genes in rapeseed.
The corticosterone-glucocorticoid receptor-AP1/CREB axis inhibits luteinizing hormone receptor expression in mouse granulosa cells
Authors: Wei Yinghui , Shen Ming 
Published: June 2020

Abstract
Background

Under stress conditions, luteinizing hormone (LH)-mediated ovulation is inhibited, resulting in insufficient oocyte production and excretion during follicular development. When the body is stressed, a large amount of corticosterone (CORT) is generated, which will lead to disorder of the body's endocrine system and damage to the body. Our previous work showed that corticosterone can block follicular development in mice. Since LH acts through binding with the LH receptor (LHR), the present study aimed to investigate whether and how CORT influence luteinizing hormone receptor expression in mouse ovarian granulosa cells (GCs).
Methods

For this purpose, 3-week-old ICR female mice were injected intraperitoneally with pregnant horse serum gonadotropin (PMSG). Meanwhile, the treatment group were injected with corticosterone corticosterone (1 mg/mouse) at intervals of 8 h; while the control group was injected with same volume of methyl sulfoxide (DMSO). Granulosa cells were collected at 24 h, 48 h, and 55 h after PMSG injection. For in vitro experiments, mice granulosa cells obtained from healthy follicles were treated with corticosterone alone, or together with inhibitors against glucocorticoid receptor (GR).
Results

The results showed that corticosterone caused down-regulation of luteinizing hormone receptor expression in granulosa cells, which was accompanied by impaired cell viability. Moreover, the effects of corticosterone was mediated by binding to its receptor in granulosa cells. Further investigations revealed that glucocorticoid receptor might regulated the transcription of luteinizing hormone receptor through inhibiting the expression of luteinizing hormone receptor transcription factors, including AP1 and CREB.
Conclusions

Our findings suggested a possible mechanism of corticosterone-induced anovulation involving the inhibition of luteinizing hormone receptor expression in granulosa cells by corticosterone-glucocorticoid receptor-AP1 / CREB axis.
Application of Biological Pretreatment using Enzyme Extracts from Filamentous Fungi to Improve Ruminal Degradability of Oil Palm Fronds.
Authors: Azmi Mohammad Azri , Yusof Mohd. Termizi , Zakaria Zunita , Mustapha Noordin Mohamed , Saad Mohd Zamri , Hassim Hasliza Abu 
Published: June 2020

Abstract
Background

Malaysia is one of the largest producer of palm oil in the world with yearly yield of 19 000 million tonnes. With its large scale production of palm oil, Malaysia produces approximately 30 million tonnes of oil palm fronds (OPF) annually. The OPF as the main agricultural by-product in Malaysia have limited use especially as ruminant feed. It is due to its poor degradability which is caused by its high lignin content. One of the most promising method to increase the utilization of OPF as ruminant feed is via biological pretreatment. Previous study has shown an increase of 12% of ruminal degradability when pretreated using enzyme extract from white rot fungi
Results

The isolation of fungi yielded 11 fungi isolates. Upon the determination of their laccase, manganese peroxidase, lignin peroxidase, carboxylmethylcellulase, avicelase and xylanase, fungi F1, F2 and F4 were selected as the fungi with desirable enzyme activity. The fungi F1, F2 and F4 were then identified as Trichoderma harzianum MK027304, Trichoderma harzianum MK027305 and Fusarium solani MK027307 respectively. The highest total gas production was produced by OPF pretreated with enzyme extract from 15 days of solid state fermentation of Trichoderma harzianum MK027304. The highest methane was produced by OPF pretreated with enzyme extract from 30 days of solid state fermentation of Trichoderma harzianum MK027305. Highest volatile acid produced by OPF pretreated with enzyme extract from Fusarium solani MK027307 after 45 days of solid state fermentation. The apparent rumen degradable carbohydrate of the pretreatment also showed the highest increase over the unpretreated OPF with increment of 35.29%.
Conclusions

The pretreatment using enzyme extract from 45 days of solid state fermentation of Fusarium solani MK027307 have shown the ability to increase the degradability of OPF by 35.29%. This finding may decrease the feeding cost of ruminant by increasing the effectiveness of utilization of OPF.
LncRNA HCG11 inhibits adipocyte differentiation in human adipose-derived mesenchymal stem cells by sponging miR-204-5p to upregulate SIRT1
Authors: Li Dandan , Liu Yang , Gao Wei , Han Jiakai , Yuan Rongrong , Zhang Mengdi , Ge Zhenying 
Published: June 2020

Abstract
Background: LncRNAs have been discovered to play a key role in adipogenesis, vital in regulating adipose developmen t. Numerous evidences show that adipogenesis is the leading cause of obesity, while the role of lncRNA HLA complex group 11 ( HCG11 ) in adipocyte differentiation has not been elucidated. Methods: hAdMSCs were used to establish a model of cell differentiation in vitro . Expression of lncRNA HCG11 was detected by RT-qPCR analysis. Transfection of the shRNA targeting HCG11 or pcDNA-HCG11 into hAdMSCs was also assessed. The adipogenic marker proteins C/EBPα, FABP4 and PPARγ2 and important inflammatory factors IL-6 and TNF-α were detected by Western blot. Bioinformatics analysis predicted the target genes of HCG11 and mir-204-5p, which was confirmed by luciferase reporter gene analysis and RNA pull-down analysis. Results: Here we show that lncRNA HCG11 was decreased as the degree of adipogenesis. The expression of C/EBPα, FABP4 and PPARγ2 were significantly downregulated transfected with pcDNA-HCG11 in hAdMSCs at different stages, while knockdown lncRNA HCG11 can promote adipocyte differentiation. In addition, miR-204-5p was a potential target gene of HCG11 and SIRT1 could directly target miR-204-5p. Overexpression of SIRT1 or transfected with agonists of SIRT1 (Res) significantly inhibited adipogenic marker protein levels and inflammatory responses, and the proliferation of hAdMSCs were also inhibited. pcDNA-HCG11 and miR-204-5p mimic were co-transfected into hAdMSCs, we found that miR-204-5p mimic reversed the suppressor effect of pcDNA-HCG11. Conclusion: Our findings showed that HCG11 negatively regulated cell proliferation, inflammatory responses and adipogenesis by miR-204-5p/SIRT1 axis. And our findings may provide a new target for the study of adipogenesis in hAdMSCs and obesity.
An Efficient Method to Detect Communities in Social Networks
Authors: KHATOON MEHJABIN , Banu W. Aisha 
Published: June 2020

Abstract
Social networks represent the social structure, which is composed of individuals having social interactions among them. The interactions between the units in a social network represent the relations of the various social contacts and aim at finding different individuals in that network, with similar interests. It is a challenging problem to detect the social interactions between individuals with comparable considerations and desires from a large social network, which can be termed as community detection. Detection of the communities from social networks has been done by other authors previously, and many community identification algorithms were also proposed, but those communities' identification has been achieved on the online available data sets. The proposed algorithm in this paper has been named as Average Degree Newman Girvan (ADNG) algorithm, which can easily identify the communities from the real-time data sets, collected from the social network websites. The approach presented here is based on first determining the average degree of the network graph and then identifying the communities using the Newman Girvan algorithm. The proposed algorithm has been compared with three community detection algorithms, i.e., Leading eigenvector (LEC) algorithm, Fastgreedy (FG) algorithm, and Kernighan-Lin (KL) algorithm based on a few metric functions. This algorithm helps to detect communities for different domains, like for any proposed government policy, online shopping products, newly launched products in a market, etc.
Does a Preoperative Cone Beam Ct Reduce Complication Rates in the Surgical Removal of Complex Lower Third Molars? A Retrospective Study Including 486 Cases
Authors: Klatt Jan , Sorowka Tony , Kluwe Lan , Smeets Ralf , Gosau Martin , Hanken Henning 
Published: June 2020

Abstract
BackroundThis study was designed to analyse the value of preoperative Cone Beam CTs prior to the surgical removal of complex lower third molars. Furthermore, the aim was to assess injuries to the inferior alveolar nerve bundle and postoperative neurological disorders depending on the position of the lower third molar and theinferior alveolar nerve bundle.MethodsPreoperative Cone Beam CTs and Orthopantomographs of 324 patients were analysed concerning the location of the lower third molars in relation to the mandible and the inferior alveolar nerve bundle. Surgery protocols of all patients who underwent the surgical removal of at least one complex lower third molar were analysed concerning patient data, length of surgery, intraoperative haemorrhage, intraoperative exposure of the inferior alveolar nerve bundle, postoperative swelling and postoperative neurological disorders. The data was then compared to data from international studies.ResultsIn all 324 patients a permanent neurological damage was not found. Temporary neurological damage was recorded in 13 cases (2.6%). A caudal nerve position with no measurable distance to the root of the lower third molar was associated with the highest risk of a temporal neurological damage. A vestibular touching nerve route also correlated with postoperative sensitivity impairment.ConclusionsThree-dimensional radiographic imaging,in our patient group, does not significantly affect the risk for complications during the surgical removal of complex lower third molars,but allows the surgeon to choose the best surgical approch for a fast and most atraumatic operation. Therefore, it should only be utilized for risk assessment, especially in cases of symptom-free lower third molars. A preoperative orthopantomogram still can be accepted as standard for radiographic imaging. The interpretation of the orthopantomogram may lead to the indication for three-dimensional imaging, if signs of increased surgery risk are present.
The risk of new-onset atrial fibrillation in patients with type 2 diabetes mellitus treated with sodium glucose cotransporter 2 inhibitors versus dipeptidyl peptidase-4 inhibitors
Authors: Ling Ann Wan-Chin , Chan Cze-Ci , Chen Shao-Wei , Kao Wei-Yi , Huang Chien-Ying , Chan Yi-Hsin , Chu Pao-Hsien 
Published: June 2020

Abstract
Purpose: Sodium glucose cotransporter 2 inhibitor (SGLT2i) reduced the risk of hard cardiovascular endpoints in type 2 diabetes mellitus (T2DM) patients with/without established cardiovascular diseases. Whether SGLT2i is associated with a lower risk of new-onset atrial fibrillation (AF) in T2DM patients is unclear. We aimed to evaluate the risk of new-onset AF associated with the use of SGLT2i compared to dipeptidyl peptidase-4 inhibitors (DPP4i) among a longitudinal cohort of diabetic patients. Methods: We used medical data from a multi-center healthcare provider in Taiwan, which included a total of 21,480 and 22,989 patients treated with SGLT2i and DPP4i, respectively, from June 1, 2016 to December 31, 2018. We used propensity-score weighting to balance covariates across study groups. Patients were followed up from the drug index date until the occurrence of new-onset AF, discontinuation of the index drug, or the end of the study period, whichever occurred first. Results: Overall, 56%, 42%, and 2% of the patients were treated with empagliflozin, dapagliflozin, and canagliflozin, respectively. Most patients in the DPP4i group were prescribed with linagliptin (51%), followed by sitagliptin (24%), saxagliptin (13%), vildagliptin (8%) and alogliptin (4%). The use of SGLT2i was associated with a lower risk of new-onset AF compared with DPP4i after propensity-score weighting [adjusted hazard ratio: 0.69; 95% confidential interval: 0.64-0.74; P < 0.001]. Subgroup analysis revealed that the use of SGLT2i was associated with a lower risk of new-onset AF compared with DPP4i across several subgroups including old age, the presence of congestive heart failure, cardiovascular disease, overweight patients, hemoglobin A1c 8%, and chronic kidney disease. The advantage of SGLT2i over DPP4i persisted with different SGLT2i (dapagliflozin or empagliflozin) and either low- or standard-dose SGLT2i. Conclusions: SGLT2i was associated with a lower risk of new-onset AF compared with DPP4i among T2DM patients in real-world practice.
Angiopoietin-like protein 4 and clinical outcomes in ischemic stroke patients
Authors: Zheng Xiaowei , Shen Suwen , Wang Aili , Zhu Zhengbao , Peng Yanbo , Peng Hao , Zhong Chongke , Guo Daoxia , Xu Tan , Chen Jing , Ju Zhong , Geng Deqin , Zhang Yonghong , He Jiang 
Published: June 2020

Abstract
Background and aims

Angiopoietin-like protein 4 (ANGPTL-4) had been reported to be associated with the risk of ischemic stroke, but its prognostic value remained unclear. The aim of this study was to investigate the association between plasma ANGPTL-4 concentrations and prognosis of ischemic stroke.

Methods

Baseline plasma ANGPTL-4 concentrations were measured in 3379 acute ischemic stroke patients. The primary outcome was a combination of death or major disability (modified Rankin Scale score, ≥3) at 3 months after ischemic stroke, and secondary outcomes included major disability, death, stroke recurrence and vascular events.

Results

At 3 months after ischemic stroke, 850 (26.16%) participants experienced major disability or died (750 major disabilities and 100 deaths). After adjusting for important covariates, odds ratios for the highest tertile of plasma ANGPTL-4 concentrations were 1.59(1.22-2.06) for primary outcome, 1.53(1.18-1.97) for major disability and 2.03(1.03-4.00) for death when compared to the lowest tertile of plasma ANGPTL-4 concentrations. For 1-SD increase of log-ANGPTL-4 concentrations (0.44 ng/ml), the adjusted odds ratios were 1.24(1.11-1.38), 1.14(1.03-1.27) and 1.72(1.32-2.23), respectively. Adding ANGPTL-4 to a model containing conventional risk factors improved risk prediction for composite outcome of death and major disability (net reclassification improvement index=11.36%, p =0.014; integrated discrimination improvement=0.22%, p =0.033). There were no significant associations between plasma ANGPTL-4 concentrations and risks of stroke recurrence and vascular events.

Conclusion

Higher plasma ANGPTL-4 concentration was associated with poor prognosis in acute ischemic stroke patients, suggesting that ANGPTL-4 might be a prognostic marker for ischemic stroke.
Sortilin levels correlate with major cardiovascular events of diabetic patients with peripheral artery disease following revascularization: a prospective study
Authors: Biscetti Federico , Nardella Elisabetta , Rando Maria Margherita , Cecchini Andrea Leonardo , Bonadia Nicola , Bruno Piergiorgio , Angelini Flavia , Stasi Carmine Di , Contegiacomo Andrea , Santoliquido Angelo , Pitocco Dario , Landolfi Raffaele , Flex Andrea 
Published: June 2020

Abstract
Background: Peripheral artery disease (PAD) represents one of the most relevant vascular complications of type 2 diabetes mellitus (T2DM). Moreover, T2DM patients suffering from PAD have an increased risk of major adverse cardiovascular events (MACE) and major adverse limb events (MALE). Sortilin, a protein involved in apolipoproteins trafficking, is associated with lower limb PAD in T2DM patients.Objective: To evaluate the relationship between baseline level of Sortilin levels, MACE and MALE occurrence after revascularization of T2DM patients with PAD and chronic limb-threatening ischemia (CLTI).Research Design and Methods: We performed a prospective non-randomized study including 230 statin-free T2DM patients with PAD and CLTI. Sortilin serum levels were measured before the endovascular intervention and incident outcomes were assessed during a 12-month follow-up.Results: Sortilin levels were significantly increased in individuals with more aggressive PAD (2.25 ± 0.51 ng/mL vs 1.44 ± 0.47 ng/mL, p < 0.001). During follow-up, 83 MACE and 116 MALE occurred. In patients, who then developed MACE and MALE, Sortilin was higher. In particular, 2.46 ± 0.53 ng/mL vs 1.55 ± 0.42 ng/mL, p < 0.001 for MACE and 2.10 ± 0.54 ng/mL vs 1.65 ± 0.65 ng/mL, p < 0.001 for MALE. After adjusting for traditional atherosclerosis risk factors, the association between Sortilin and vascular outcomes remained significant in a multivariate analysis. In our receiver operating characteristics (ROC) curve analysis using Sortilin levels the prediction of MACE incidence improved [area under the curve (AUC) = 0.94] and MALE (AUC = 0.72).Conclusions: This study demonstrates that Sortilin correlates with incidence of MACE and MALE after endovascular revascularization in a diabetic population with PAD and CLTI.
Vibration Characteristics of Rotor System with Loose Disc Caused by the Insufficient Interference Force
Authors: Li Zhi-Nong , Qiao Fang , Lu Wen Xiu , Liu Jie , Wang Dong , Chu Fu Lei 
Published: June 2020

Abstract
The loose of mechanical parts is one of the common failures in rotating machinery. The current researches of loose fault mainly focus on non-rotating components. However, the loose of disc, which is the main work part in the rotor system, is less paid attention, and the mechanism and dynamics characteristics of the loose fault are also almost ignored. In this paper, a dynamic contact model of the rotor system with loose disc is established considering the microscopic surface topography. Through the numerical simulation, the vibration characteristics of the disc-shaft rotor system are analyzed and discussed. The simulation results are further verified by experiments. The results show that the rotation state of the disc is affected by the rotation speed of the shaft, contact stiffness, a gap between the disc and shaft, damping of the disc, and the rotational damping. When the speed difference between the disc and shaft is zero or large, the collision frequency is mainly composed of one frequency component. When the rotational speed of the disc approaches the shaft, the beating vibration phenomenon of the disc occurs in the horizontal direction. As the decreases of relative speed between the disc and shaft, the disc trajectory changes from ‘circular’ to ‘double ring’ and then ‘circular’. The research results on nonlinear dynamics characteristics of the loose disc has important theoretical value and practical application value, and makes up for its shortcomings in the rotor system with loose disc.
Self-managed medication abortion outcomes: results from a prospective pilot study
Authors: Moseson Heidi , Jayaweera Ruvani , Raifman Sarah , Keefe-Oates Brianna , Filippa Sofia , Motana Relebohile , Egwuatu Ijeoma , Grosso Belén , Kristianingrum Ika , Nmezi Sybil , Zurbriggen Ruth , Gerdts Caitlin 
Published: June 2020

Abstract
Background: To evaluate the feasibility of conducting a prospective study to measure self-managed medication abortion outcomes, and to collect preliminary data on safety and effectiveness of self-managed medication abortion, we recruited callers to accompaniment groups (volunteer networks that provide counselling through the out-of-clinic medication abortion process by trained counselors over the phone or in-person).Methods: In 2019, we enrolled callers to three abortion accompaniment groups in three countries into a prospective study on the safety and effectiveness of self-managed medication abortion with accompaniment support. Participants completed up to five interview-administered questionnaires from baseline through six-weeks after taking the pills. Primary outcomes included: (1) the proportion that had a complete abortion; and (2) the proportion who experienced any warning signs of potential or actual complications.Results: Over the 30-day recruitment period, we enrolled 227 participants. At the one-week follow-up, two participants (1%) reported a miscarriage prior to taking the pills, and 202 participants (89% of those enrolled and 99% of those who participated in the one-week survey) had obtained and taken the medications. Three weeks after taking the medications, 192 (95%) participants reported feeling that their abortion was complete, and 189 (94%) felt their abortion was complete and had not reported a surgical intervention. Three (1.5%) received a surgical intervention, two (1%) received antibiotics, and five (3%) other medications. Participants did not report any major adverse events.Conclusion: These results establish the feasibility of conducting prospective studies of self-managed medication abortion in legally restrictive settings. Further, these prospectively collected pilot data suggest that self-managed medication abortion with accompaniment support may be safe and effective. Trial Registration: ISRCTN95769543