bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disentangling the Genetics of Sarcopenia: prioritization of NUDT3 and KLF5 as genes for lean mass and HLA-DQB1-AS1 for hand grip strength based on associated SNPs
Authors: Singh Abhishek N. , Gasman Bili 
Published: January 2020

Abstract
Background: Sarcopenia is a skeletal muscle disease of clinical importance that occurs commonly in old age and in various disease sub-categories. Widening the scope of knowledge of the genetics of muscle mass and strength is important because it may allow to identify patients with an increased risk to develop a specific musculoskeletal disease or condition such as sarcopenia based on genetic markers. We used bioinformatics tools to identify gene loci responsible for regulating muscle strength and lean mass, which can then be a target for downstream lab experimentation validation. Single nuclear polymorphisms (SNPs) associated with various disease traits of muscles and specific genes were chosen according to their muscle phenotype association p-value, as traditionally done in Genome Wide Association Studies, GWAS. We've developed and applied a combination of expression quantitative trait loci (eQTLs) and GWAS summary information, to prioritize causative SNP and point out the unique genes associated in the tissues of interest (muscle). Results: We found NUDT3 and KLF5 for lean mass and HLA-DQB1-AS1 for hand grip strength as candidate genes to target for these phenotypes. The associated regulatory SNPs are rs464553, rs1028883 and rs3129753 respectively. Conclusion: Transcriptome Wide Association Studies, TWAS, approaches of combining GWAS and eQTL summary statistics proved helpful in statistically prioritizing genes and their associated SNPs for the disease phenotype of study, in this case, Sarcopenia. Potentially regulatory SNPs associated with these genes can be then wet-lab verified, depending on the phenotype it is hypothesized to affect.
Pulmonary microbiome patterns correlate with the course of the disease in patients with sepsis-induced ARDS
Authors: Schmitt Felix Carl Fabian , Lipinski Anna , Hofer Stefan , Uhle Florian , Nusshag Christian , Hackert Thilo , Dalpke Alexander H. , Weigand Markus A. , Brenner Thorsten , Boutin Sébastien 
Published: January 2020

Abstract
The authors have withdrawn this preprint from Research Square
Modified-ramped position: a new position for intubation of obese females. A randomized controlled pilot study
Authors: hasanin ahmed , Tarek Hager , Mostafa Maha MA , Arafa Amany , Safina Ahmed G , Elsherbiny Mona H , Hosny Osama , Gado Ahmed A , Almenesey Tarek , Hamden Ghada Adel , Mahmoud Mohamed , Amin Sarah 
Published: January 2020

Abstract
Background
Endotracheal intubation requires optimum positioning of the head and neck. In obese females, the usual ramped position might not provide adequate intubating conditions. We hypothesized that a new position, termed modified-ramped position, during induction of anesthesia would facilitate endotracheal intubation through shifting the breasts away from the laryngoscope and also improve the laryngeal visualization.
Methods
Sixty obese female patients scheduled for general anesthesia were randomly assigned into either ramped or modified-ramped position during induction of anesthesia. In the ramped position (n=30), the patient’s head and shoulders were elevated to achieve alignment of the sternal notch and the external auditory meatus; while in the modified-ramped position (n=30), the patient’s shoulders were elevated using a special pillow, and the head was extended to the widest possible range. Our primary outcome was the incidence of failed laryngoscopic insertion in the oral cavity (the need for patient repositioning). Other outcomes included time till vocal cord visualization, time till successful endotracheal intubation, difficulty of the mask ventilation, and Cormack-Lehane grade for laryngeal view.
Results
Fourteen patients (47%) in the ramped group required repositioning to facilitate introduction of the laryngoscope in the oral cavity, in comparison to one patient (3%) in the modified-ramped position (p<0.001). Modified-ramped position showed a lower incidence of difficult mask ventilation, shorter time for glottic visualization, and shorter time for endotracheal tube insertion compared to the ramped position. The Cormack-Lehane grade was better in the modified-ramped position.
Conclusion
Modified-ramped position provided better intubating conditions, improved the laryngeal view, and eliminated the need for repositioning of obese female patients during insertion of the laryngoscope compared to the ramped position.
ADL Recovery Trajectory after Discharge and its Predictors among Baseline-Independent Older Inpatients
Authors: Li Xiuyue , Zheng Tingting , Guan Yaqi , Li Hui , Zhu Kexin , Shen Lu , Yin Zhiqin 
Published: January 2020

Abstract

Background Among the previous studies about the ADL recovery and its predictors, the researches and resources used to study and protect the baseline-independent older patients from being permanently ADL-dependent was few. We aimed to describe the level of activities of daily living (ADL) at discharge and ADL change within 6 months after discharge in older patients who were ADL-independent before admission but became dependent because of acute illness, and to identify the predictors of early rehabilitation,so as to provide the basis to early intervention. Methods Stratified cluster sampling was used to recruit 520 hospitalised older patients who were ADL-independent from departments of internal medicine at two tertiary hospitals from August 2017 to May 2018. Demographics, clinical data, and ADL status at 1, 3, and 6 months after discharge were collected. Data were analysed using descriptive statistics, Student’s t-test, Pearson’s chi-square test,Spearman’s correlation analysis, binary logistic regression analysis, and receiver operating characteristic (ROC) curve analysis. Results There were 403 out of 520 patients completing the 6-month follow-up, and 229 (56.8%) regained independence at 6 months after discharge. There was an overall increasing trend in ADL with time. The recovery rate was the highest within the first month after discharge, gradually declined after 1 month, and changed less obviously from 3 to 6 months after discharge (p<0.001). ADL score at discharge (OR=1.034, p<0.001), age (OR=0.269, p=0.001), post-discharge residence (OR=0.390, p<0.05), and cognition status at discharge (OR=1.685, p<0.05) were predictors of ADL recovery. The area under the curve of the four predictors combined was 0.763 (p<0.001). Conclusion Studying ADL recovery rate and its predicting indicators of the baseline independent inpatients at different time points provide a theoretical reference for the formulation of nursing plans and allocation of care resources.
Performance, metabolic and hormonal responses of grazing Nellore cows to an energy-protein supplementation during the pre-partum phase
Authors: Ferreira Matheus Fellipe de Lana , Rennó Luciana Navajas , Detmann Edenio , Paulino Mário Fonseca , Filho Sebastião de Campos Valadares , Moreira Samira Silveira , Martins Hudson Caio , de Oliveira Bruno Inácio Correa , Marquez Julia Avansi , Cidrine Isabela de Paula 
Published: January 2020

Abstract

Background: Supplementation programs for prepartum beef cows are usually adopted because the nutritional status of the cows upon calving is a main factor impacting reproductive performance of the next production cycle. Metabolic parameters that relate nutritional status to physiological processes can be used to better understand the effects of supplementation. This study evaluated the effects of 60-d pre-partum energy-protein supplementation on performance, metabolic and hormonal responses during the peripartum phase of grazing beef cows. Thirty-eight pregnant multiparous Nellore cows were assigned to a completely randomized design with two treatments: control (no supplement) and supplementation (1.5 kg of energy-protein per d) during the last 60 d of gestation. Results: The supplemented cows had higher ADG pre-partum (P<0.10), but postpartum ADG did not differ between treatments. Nonsupplemented cows’ ADG did not change during these periods (P>0.10). Supplementation did not affect BCS and calf BW upon calving on days 45 and 90, milk yield and composition (P>0.10). No differences were found for forage intake and neutral detergent fiber digestibility (P>0.10). The intake and digestibility of CP and OM increased with supplementation (P<0.10). An interaction occurred (P>0.10) between supplementation and peripartum days for BUN, βHB, T3 and T4. Concentration of others blood parameters significantly changed (P<0.10) along peripartum days. There was no difference in pregnancy rates and days from calving to conception among treatments (P>0.10). Conclusions: Providing an energy and protein supplement to grazing Nellore cows over the last 60 d of gestation improved their pre-partum energy balance. However, no post-partum carryover effects were detected. Keywords: nutrition, metabolism, parturition, Zebu
Access to and Use of Health and Social Services among People who Inject Drugs in two urban areas of Mozambique, 2014: Qualitative Results from a Formative Assessment
Authors: Dengo-Baloi Liliana Candida , Boothe Makini Aida , Semá-Baltazar Cynthia Amino , Sathane Isabel , Langa Denise Chitsondzo , Condula Manuel , Ricardo Helena , Inguane Celso , Teodoro Eugénia , Gouveia Lídia , Raymond Henry F. , Horth Roberta 
Published: January 2020

Abstract
Background: Prior to 2014, data about health seeking behaviors or service uptake for People who inject drugs (PWID) in Mozambique did not exist. We present the results from the formative assessment component of the Biological and Behavioral Survey (BBS). Methods: Standardized interview guides were used during key informant interviews (KII) and focus group discussions (FGD) in Maputo and Nampula/Nacala to discuss issues related to risk behaviors and access to and utilization of health and social services by PWID. The target sample size was not defined a priori, but instead KII and FGD were conducted until responses reached saturation. Data analysis was based on the principles of grounded theory related to qualitative research. Results: Eighty-eight respondents, ages 15 to 60, participated in KIIs and FGDs. Participants were majority male from diverse income and education levels and included current and former PWID, non-injection drug users, health and social service providers, peer educators, and community health workers. Respondents reported that PWID engage in high-risk behaviors such as needle and syringe sharing, exchange of sex for drugs or money, and low condom use. According to participants, PWID would rather rent, share or borrow injection equipment at shooting galleries than purchase them due to stigma, fear of criminalization, transportation and purchase costs, restricted pharmacy hours, personal preference for needle sharing, and immediacy of drug need. Barriers to access and utilization of health and social services include distance, requirements to represent identification documents and fear of criminalization, the limited availability of programs for PWID, lack of knowledge of the few programs that exist, concerns about the quality of care provided by health providers, lack of readiness as a result of addiction and perceived stigma related to the use of mental health services offering treatment to PWID. Conclusions: Mozambique urgently needs to establish specialized harm reduction programs for PWID and improve awareness of available resources. Services should be located in hot spot areas to address issues related to distance, transportation and the planning required for safe injection. Specific attention should go to the creation of PWID-focused health and social services outside of state-sponsored psychiatric treatment centers.
Prevalence of sarcopenia in multi ethnics adults and the association with cognitive impairment: findings from West-China Health and Aging Trend Study
Authors: Liu Xiaolei , Hou Lisha , Xia Xin , Liu Yang , Zuo Zhiliang , Zhang Yan , Zhao Wanyu , Hao Qiukui , Yue Jirong , Dong Birong 
Published: January 2020

Abstract
Background Sarcopenia is a condition that is characterized by loss of muscle mass, muscle strength and muscle functional impairment with ageing. It is associated with poor health outcomes, premature death and a significant burden on the global health economy. The prevalence of sarcopenia in China is unknown since most of the studies are lack of uniform standard. The study was undertaken to study the prevalence of sarcopenia and the association with cognitive impairment among multi-ethnic adults aged 50 years old or older in western China. Methods We measured gait speed, handgrip strength and muscle mass by using bioelectrical impedance analysis (BIA) for all eligible participants and 4500 participants were eligible for the analysis. We defined sarcopenia using the diagnostic algorithm recommended by the Asian Working Group for Sarcopenia (AWGS) . We assessed the participants' cognitive functions using the 10-item Short Portable Mental Status Questionnaire (SPMSQ) . Relationships between sarcopenia and cognitive impairment were analyzed using univariate and multivariate analyses. Results Of 4500 participants (mean age 62.4 ± 8.3 years), 869 (19.31%) adults were sarcopenia. 446 (9.9%) participants were identified as having mild cognitive impairment, 144 (3.2%) adults were identified as having moderate/severe cognitive impairment. After adjusting for age, gender, ethnics and other potential cofounders, cognitive impairment was found to be independently associated with sarcopenia with a dosage effect (mild cognitive impairment: odds ratio [OR] : 1.52, 95% CI 1.18–1.95; moderate/severe cognitive impairment: OR: 3.26, 95% CI 2.23–4.76). After gender stratification, the association between mild cognitive impairment with sarcopenia in male is not significant, while is still significant in female. While the association between moderate/severe cognitive impairment is independently associated with sarcopenia in both male and female. Conclusions The prevalence rates of sarcopenia, mild cognitive impairment, moderate/severe cognitive impairment among the communities aged 50 or older in western China were 19.31%, 9.9% and 3.2%, respectively. Cognitive impairment was significantly associated with sarcopenia with a dosage effect, especially in female.
Correlation between detergent activity and anti-herpes simplex virus-2 activity of commercially available vaginal gels
Authors: Szöllősi Andrea , Raffai Tímea , Bogdanov Anita , Endrész Valéria , Párducz László , Somogyvári Ferenc , Janovák László , Burián Katalin , Virok Dezső Peter 
Published: January 2020

Abstract
Objective Herpes simplex virus-2 (HSV-2) infections are almost exclusively sexually transmitted. The presence of vaginal gels during the sexual activity may have a significant positive or negative impact on the viral transmission. Therefore we investigated three off-the-shelf vaginal lubricants and one pH restoring gel to evaluate their impact on HSV-2 replication.

Results HeLa cells were infected with untreated virions and virions incubated with the particular gels. The accumulation of viral genomes was monitored by quantitative PCR (qPCR) method at 24 hours post infection. Two of the tested gels had no significant effect on HSV-2 replication at the maximum applied concentration, while two had a strong inhibitory effect (~ 98% reduction of replication). The replication inhibitory effect was observed at various multiplicity of infection (MOI 0.4-6.4) and the two inhibitory gels were also capable of inhibiting the HSV-2 induced cytopathic effect on HeLa cells. The surface tension decreasing activity - a sign of detergent activity - was strongly correlated with the anti-HSV-2 activity of the gels (R 2 : 0.88). Our results indicate that off-the-shelf vaginal gels have a markedly different anti-HSV-2 activity that may influence HSV-2 transmission.
Efficacy and safety of lanreotide autogel compared with lanreotide 40 mg prolonged release in Chinese patients with active acromegaly: Results from a phase 3, prospective, randomized, and open-label study (LANTERN)
Authors: An Zhenmei , Lei Ting , Duan Lian , Hu Pei , Gou Zhongping , Zhang Lihui , Durand-Gasselin Lucie , Wang Nan , Wang Yan , Gu Feng 
Published: January 2020

Abstract
Background: Lanreotide autogel is a somatostatin analog (SSA) approved for the treatment of acromegaly in 73 countries worldwide; however, it is not yet approved in China. The aim of this study was to evaluate the efficacy and safety of lanreotide autogel compared with lanreotide 40 mg prolonged release (PR) in Chinese patients with active acromegaly. Methods: LANTERN was a Phase 3, randomized, open-label, non-inferiority study. Patients with active acromegaly who had undergone surgery ≥3 months prior, or were unlikely or unable to undergo surgery, were treated with lanreotide autogel 60/90/120 mg (monthly deep subcutaneous injection) or lanreotide 40 mg PR (intramuscular injection every 7, 10, or 14 days) for 32 weeks. Primary endpoint was mean change-from-baseline in age-adjusted insulin-like growth factor-1 (IGF-1) standard deviations scores (SDS) at the end-of-study. Secondary endpoints included: growth hormone (GH) levels ≤2.5 µg/L or ≤1.0 µg/L, ≥20% reduction in tumor volume (TV) and safety. Results: In total, 128 patients were randomized and received study treatment. Lanreotide autogel was non-inferior to lanreotide 40 mg PR: treatment difference (95% CI) for IGF-1 SDS between groups was −0.32 (−0.74, 0.11; per protocol population) and −0.27 (−0.63, 0.09; intention-to-treat [ITT] population), respectively. Reductions in IGF-1 (−6.453 vs −7.003) and GH levels (−9.548 µg/L vs −13.182 µg/L), and the proportion of patients with ≥1 acromegaly symptom (−20.3% vs −32.5%) were observed from baseline to end-of-study in lanreotide autogel and lanreotide 40 mg PR groups, respectively. In the lanreotide autogel group, 45.5% (25/55) patients achieved ≥20% reduction in TV compared with 50.9% (25/53) in lanreotide 40 mg PR group (ITT). Safety profiles were similar in both treatment groups. Conclusions: Lanreotide autogel was non-inferior to lanreotide 40 mg PR in Chinese patients with active acromegaly after 32 weeks of treatment.
The paradox on declining job satisfaction trend with the intention to remain employed in the nursing profession in the Ethiopian nursing workforce. Systematic review and meta-analysis
Authors: kassahun Chanyalew Worku , Ewunitie Amare Wondim 
Published: January 2020

Abstract
Background: High attrition rates, low job satisfaction and motivation are great concern that pose major challenges for nurses in Ethiopia where it has unfavorable influences on the nursing system and the quality of nursing care. But, different authors reported inconsistent findings about the proportion of job satisfaction and intention to remain employed. In addition; the trends and national pooled estimate of proportion of job satisfaction and intention to remain employed in nursing profession is no assessed. Therefore; the aim of this systemic review and meta-analysis is to show the trends, and estimate the national pooled proportion of job satisfaction and intention to remain employed in nursing profession in Ethiopia. Methods: Major databases; PubMed, Medline and African Journals Online were included in the review. Grey literatures like Web of Science, Google scholar and repository online library in universities in Ethiopian were searched. Joanna Briggs Institute Meta-Analysis of Statistics Assessment and Review Instrument was used for critical appraisal. The meta-analysis was conducted using STATA-11. Descriptive information of studies was presented in narrative form and quantitative results were presented in forest plots. The Cochran Q test and I 2 test statistics were used to survey heterogeneity among studies. The pooled estimate prevalence with 95% confidence intervals were computed. Results: A total seven and five studies were included to estimate job satisfaction and intention to be employed respectively. The proportion of job satisfaction is declining from 2014 (59.6%) to 2019 (48%) while the proportion of intention to remain employed in nursing profession is increasing from 2014 (39%) to 2017 (86.7%). The overall national pooled proportion of job satisfaction among nurses was 54.96 % (95% CI: 48.83%, 61%) while the intention to remain employed in nursing profession was 54.94 % (95% CI: 36.44%, 73.45%). Conclusions: There is declining of job satisfaction and increasing of intention to remain employed trends across publication years. But, the national pooled proportion of job satisfaction and intention to remain employed are low. Interventions aiming to increases the job satisfaction and intention to remain employed is important. Keywords: Job satisfaction, Intention to remain employed, Nurses, Ethiopia.
Integrated omics approach to understand Pistacia - aphid gall development
Authors: Narendran Pavithra , Begum Noorunnisa , Bhatt Amit , Kukkupuni Subrahmanya Kumar , Venkatasubramanian Padma , Prasad Thottethodi Subrahmanya Keshava , Najar Mohd Altaf , Arunkumar Kallare P , JLN Sastry , S Badrinarayan , Gowda Malali 
Published: January 2020

Abstract
Background: Pistacia chinensis subsp. integerrima (J. L. Stewart ex Brandis) Rech. f. belongs to family Anacardiaceae, is well known for usage of galls in traditional medicine. The galls in the apical meristem of plants are induced by aphid Baizongia pistaciae L infestation. The Pistacia galls are used in Ayurvedic formulations for the treatment of cough and respiratory diseases, loss of appetite, dyspeptic vomiting and dysentery. The drastic decrease in the gall formation in recent decades is possibly due to climate change or human interventions, making it an important species from conservation point of view. There is not much work carried out at molecular level to understand the gall development. In this study, we obtained molecular insight on insect and plant through genomic, transcriptomic and proteomic approaches. Results: We sequenced the whole genome of Pistacia genome using Illumina sequencing platform. The assembly genome size of Pistacia was 549 Mb with 80688 scaffolds at N50 = 12607 nts. A total of 51290 genes were from Pistacia genome annotation. The transcriptome analysis revealed of 76186 and 46327 transcripts from gall and leaf, respectively. The ethylene responsive transcription factor families were induced abundantly in galls including GATA, bHLH, MYB, BZIP, Trihelix, MADS, B3 domain. In addition, we have also obtained highly expressed genes in gall for biosynthesis of secondary metabolites, plant-aphid interactions, stress responses, phytohormone signal transduction and terpene biosynthesis. We have identified 21 proteins against Cajanus cajan , 10 proteins against Arabidopsis thaliana and 14 proteins against Drosophila melanogaster . We also identified abundant peptides for actin and tubulin of aphid in the gall tissue. Conclusion: This study provides a basis for the understanding of the genes expressed in gall when compared to leaf and also genes required for gall induction and development. We hypothesed that actin and microtubule proteins are from aphids origin as we found that transcript TRINITY_DN180182_c0_g1_i1 mapped to Acyrthosiphon pisum tubulin beta-1 (Tub1), mRNA with 75 % homology.