bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intermittent fasting from dawn to sunset for four consecutive weeks induces anticancer serum proteome response and improves metabolic syndrome
Authors: Mindikoglu Ayse L. , Abdulsada Mustafa M. , Jain Antrix , Jalal Prasun K. , Devaraj Sridevi , Wilhelm Zoe R. , Opekun Antone R. , Jung Sung Yun 
Published: October 2020

AbstractMetabolic syndrome is characterized by central obesity, insulin resistance, elevated blood pressure, and dyslipidemia. Metabolic syndrome is a significant risk factor for several common cancers (e.g., liver, colorectal, breast, pancreas). Pharmacologic treatments used for the components of the metabolic syndrome appear to be insufficient to control cancer development in subjects with metabolic syndrome. Murine models showed that cancer has the slowest progression when there is no food consumption during the daily activity phase. Intermittent fasting from dawn to sunset is a form of fasting practiced during human activity hours. To test the anticancer effect of intermittent fasting from dawn to sunset in metabolic syndrome, we conducted a pilot study in 14 subjects with metabolic syndrome who fasted (no eating or drinking) from dawn to sunset for more than 14 h daily for four consecutive weeks. We collected serum samples before 4-week intermittent fasting, at the end of 4th week during 4-week intermittent fasting and 1 week after 4-week intermittent fasting. We performed serum proteomic analysis using nano ultra-high performance liquid chromatography-tandem mass spectrometry. We found a significant fold increase in the levels of several tumor suppressor and DNA repair gene protein products (GP)s at the end of 4th week during 4-week intermittent fasting (CALU, INTS6, KIT, CROCC, PIGR), and 1 week after 4-week intermittent fasting (CALU, CALR, IGFBP4, SEMA4B) compared with the levels before 4-week intermittent fasting. We also found a significant reduction in the levels of tumor promoter GPs at the end of 4th week during 4-week intermittent fasting (POLK, CD109, CAMP, NIFK, SRGN), and 1 week after 4-week intermittent fasting (CAMP, PLAC1) compared with the levels before 4-week intermittent fasting. Fasting from dawn to sunset for four weeks also induced an anti-diabetes proteome response by upregulating the key regulatory proteins of insulin signaling at the end of 4th week during 4-week intermittent fasting (VPS8, POLRMT, IGFBP-5) and 1 week after 4-week intermittent fasting (PRKCSH), and an anti-aging proteome response by upregulating H2B histone proteins 1 week after 4-week intermittent fasting. Subjects had a significant reduction in body mass index, waist circumference, and improvement in blood pressure that co-occurred with the anticancer, anti-diabetes, and anti-aging serum proteome response. These findings suggest that intermittent fasting from dawn to sunset actively modulates the respective genes and can be an adjunct treatment in metabolic syndrome. Further studies are needed to test the intermittent fasting from dawn to sunset in the prevention and treatment of metabolic syndrome-induced cancers.
A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice
Authors: Ihara Kensuke , Sasano Tetsuo , Hiraoka Yuichi , Togo-Ohno Marina , Soejima Yurie , Sawabe Motoji , Tsuchiya Megumi , Ogawa Hidesato , Furukawa Tetsushi , Kuroyanagi Hidehito 
Published: October 2020

AbstractDilated cardiomyopathy (DCM) is a fatal heart disease characterized by left ventricular dilatation and cardiac dysfunction. Recent genetic studies on DCM have identified causative mutations in over 60 genes, including RBM20, which encodes a regulator of heart-specific splicing. DCM patients with RBM20 mutations have been reported to present with more severe cardiac phenotypes, including impaired cardiac function, atrial fibrillation (AF), and ventricular arrhythmias leading to sudden cardiac death, compared to those with mutations in the other genes. An RSRSP stretch of RBM20, a hotspot of missense mutations found in patients with idiopathic DCM, functions as a crucial part of its nuclear localization signals. However, the relationship between mutations in the RSRSP stretch and cardiac phenotypes has never been assessed in an animal model. Here, we show that Rbm20 mutant mice harboring a missense mutation S637A in the RSRSP stretch, mimicking that in a DCM patient, demonstrated severe cardiac dysfunction and spontaneous AF and ventricular arrhythmias mimicking the clinical state in patients. In contrast, Rbm20 mutant mice with frame-shifting deletion demonstrated less severe phenotypes, although loss of RBM20-dependent alternative splicing was indistinguishable. RBM20S637A protein cannot be localized to the nuclear speckles, but accumulated in cytoplasmic, perinuclear granule-like structures in cardiomyocytes, which might contribute to the more severe cardiac phenotypes.
Effects of long-lasting social isolation and re-socialization on cognitive performance and brain activity: a longitudinal study in Octodon degus
Authors: Rivera Daniela S. , Lindsay Carolina B. , Oliva Carolina A. , Codocedo Juan Francisco , Bozinovic Francisco , Inestrosa Nibaldo C. 
Published: October 2020

AbstractSocial isolation is considered a stressful situation that results in increased physiological reactivity to novel stimuli, altered behaviour, and impaired brain function. Here, we investigated the effects of long-term social isolation on working memory, spatial learning/memory, hippocampal synaptic transmission, and synaptic proteins in the brain of adult female and male Octodon degus. The strong similarity between degus and humans in social, metabolic, biochemical, and cognitive aspects, makes it a unique animal model that can be highly applicable for further social, emotional, cognitive, and aging studies. These animals were socially isolated from post-natal and post-weaning until adulthood. We also evaluated if re-socialization would be able to compensate for reactive stress responses in chronically stressed animals. We showed that long-term social isolation impaired the HPA axis negative feedback loop, which can be related to cognitive deficits observed in chronically stressed animals. Notably, re-socialization restored it. In addition, we measured physiological aspects of synaptic transmission, where chronically stressed males showed more efficient transmission but deficient plasticity, as the reverse was true on females. Finally, we analysed synaptic and canonical Wnt signalling proteins in the hypothalamus, hippocampus, and prefrontal cortex, finding both sex- and brain structure-dependent modulation, including transient and permanent changes dependent on stress treatment.
Experimental adaptation of dengue virus 1 to Aedes albopictus mosquitoes by in vivo selection
Authors: Bellone Rachel , Lequime Sebastian , Jupille Henri , Göertz Giel P. , Aubry Fabien , Mousson Laurence , Piorkowski Géraldine , Yen Pei-Shi , Gabiane Gaelle , Vazeille Marie , Sakuntabhai Anavaj , Pijlman Gorben P. , de Lamballerie Xavier , Lambrechts Louis , Failloux Anna-Bella 
Published: October 2020

AbstractIn most of the world, Dengue virus (DENV) is mainly transmitted by the mosquito Aedes aegypti while in Europe, Aedes albopictus is responsible for human DENV cases since 2010. Identifying mutations that make DENV more competent for transmission by Ae. albopictus will help to predict emergence of epidemic strains. Ten serial passages in vivo in Ae. albopictus led to select DENV-1 strains with greater infectivity for this vector in vivo and in cultured mosquito cells. These changes were mediated by multiple adaptive mutations in the virus genome, including a mutation at position 10,418 in the DENV 3′UTR within an RNA stem-loop structure involved in subgenomic flavivirus RNA production. Using reverse genetics, we showed that the 10,418 mutation alone does not confer a detectable increase in transmission efficiency in vivo. These results reveal the complex adaptive landscape of DENV transmission by mosquitoes and emphasize the role of epistasis in shaping evolutionary trajectories of DENV variants.
An in-silico layer-by-layer adsorption study of the interaction between Rebaudioside A and the T1R2 human sweet taste receptor: modelling and biosensing perspectives
Authors: Arodola Olayide A. , Kanchi Suvardhan , Hloma Phathisanani , Bisetty Krishna , Asiri Abdullah M. , Inamuddin  
Published: October 2020

AbstractThe human sweet taste receptor (T1R2) monomer—a member of the G-protein coupled receptor family that detects a wide variety of chemically and structurally diverse sweet tasting molecules, is known to pose a significant threat to human health. Protein that lack crystal structure is a challenge in structure-based protein design. This study focused on the interaction of the T1R2 monomer with rebaudioside A (Reb-A), a steviol glycoside with potential use as a natural sweetener using in-silico and biosensing methods. Herein, homology modelling, docking studies, and molecular dynamics simulations were applied to elucidate the interaction between Reb-A and the T1R2 monomer. In addition, the electrochemical sensing of the immobilised T1R2-Reb-A complex with zinc oxide nanoparticles (ZnONPs) and graphene oxide (GO) were assessed by testing the performance of multiwalled carbon nanotube (MWCNT) as an adsorbent experimentally. Results indicate a strong interaction between Reb-A and the T1R2 receptor, revealing the stabilizing interaction of the amino acids with the Reb-A by hydrogen bonds with the hydroxyl groups of the glucose moieties, along with a significant amount of hydrophobic interactions. Moreover, the presence of the MWCNT as an anchor confirms the adsorption strength of the T1R2-Reb-A complex onto the GO nanocomposite and supported with electrochemical measurements. Overall, this study could serve as a cornerstone in the development of electrochemical immunosensor for the detection of Reb-A, with applications in the food industry.
Potential energy of complex networks: a quantum mechanical perspective
Authors: Amoroso Nicola , Bellantuono Loredana , Pascazio Saverio , Lombardi Angela , Monaco Alfonso , Tangaro Sabina , Bellotti Roberto 
Published: October 2020

AbstractWe propose a characterization of complex networks, based on the potential of an associated Schrödinger equation. The potential is designed so that the energy spectrum of the Schrödinger equation coincides with the graph spectrum of the normalized Laplacian. Crucial information is retained in the reconstructed potential, which provides a compact representation of the properties of the network structure. The median potential over several random network realizations, which we call ensemble potential, is fitted via a Landau-like function, and its length scale is found to diverge as the critical connection probability is approached from above. The ruggedness of the ensemble potential profile is quantified by using the Higuchi fractal dimension, which displays a maximum at the critical connection probability. This demonstrates that this technique can be successfully employed in the study of random networks, as an alternative indicator of the percolation phase transition. We apply the proposed approach to the investigation of real-world networks describing infrastructures (US power grid). Curiously, although no notion of phase transition can be given for such networks, the fractality of the ensemble potential displays signatures of criticality. We also show that standard techniques (such as the scaling features of the largest connected component) do not detect any signature or remnant of criticality.
Magnetism and ion diffusion in honeycomb layered oxide $${\hbox {K}_2\hbox {Ni}_2\hbox {TeO}_6}$$
Authors: Matsubara Nami , Nocerino Elisabetta , Forslund Ola Kenji , Zubayer Anton , Papadopoulos Konstantinos , Andreica Daniel , Sugiyama Jun , Palm Rasmus , Guguchia Zurab , Cottrell Stephen P. , Kamiyama Takashi , Saito Takashi , Kalaboukhov Alexei , Sassa Yasmine , Masese Titus , Månsson Martin 
Published: October 2020

AbstractIn the quest for developing novel and efficient batteries, a great interest has been raised for sustainable K-based honeycomb layer oxide materials, both for their application in energy devices as well as for their fundamental material properties. A key issue in the realization of efficient batteries based on such compounds, is to understand the K-ion diffusion mechanism. However, investigation of potassium-ion (K$$^+$$

+

) dynamics in materials using e.g. NMR and related techniques has so far been very challenging, due to its inherently weak nuclear magnetic moment, in contrast to other alkali ions such as lithium and sodium. Spin-polarised muons, having a high gyromagnetic ratio, make the muon spin rotation and relaxation ($$\mu ^+$$

μ
+

SR) technique ideal for probing ions dynamics in these types of energy materials. Here we present a study of the low-temperature magnetic properties as well as K$$^+$$

+

dynamics in honeycomb layered oxide material $${\hbox {K}_2\hbox {Ni}_2\hbox {TeO}_6}$$

K
2

Ni
2

TeO
6

using mainly the $$\mu ^+$$

μ
+

SR technique. Our low-temperature $$\mu ^+$$

μ
+

SR results together with complementary magnetic susceptibility measurements find an antiferromagnetic transition at $$T_{\mathrm{N}}\approx 27$$

T
N

≈
27

K. Further $${\mu}^{+}$$

μ

+

SR studies performed at higher temperatures reveal that potassium ions (K$$^+$$

+

) become mobile above 200 K and the activation energy for the diffusion process is obtained as $$E_{\mathrm{a}}=121 (13)$$

E
a

=
121

(
13
)

meV. This is the first time that K$$^+$$

+

dynamics in potassium-based battery materials has been measured using $$\mu ^+$$

μ
+

SR. Assisted by high-resolution neutron diffraction, the temperature dependence of the K-ion self diffusion constant is also extracted. Finally our results also reveal that K-ion diffusion occurs predominantly at the surface of the powder particles. This opens future possibilities for potentially improving ion diffusion as well as K-ion battery device performance using nano-structuring and surface coatings of the particles.
Lightweight design and static analysis of lattice compressor impeller
Authors: Zhang Yuan , Li Fanchun , Jia Dejun 
Published: October 2020

AbstractTaking the compressor impeller as the research object and the lightweight design as the research goal, a lattice filled lattice cell suitable for the application of rotating periodic symmetric structure is designed. Its purpose is to make the rigidity and strength of impeller adjustable and reduce the mass of impeller on the premise of meeting the design requirements. The analysis and comparison of unfilled impeller, solid impeller and lattice filled impeller with different diameters were carried out under the limit condition of 80,000 r/min. The results showed that the average circumferential deformation of lattice impeller tip with beam diameter of 0.2 mm, 0.4 mm and 1 mm was 4.84%, 3.49% and 3.71% lower than that of solid impeller. For the impeller with a lattice beam diameter of 0.4 mm, its weight can be reduced by 22.68% compared with the solid impeller. The average circumferential deformation of the tip of the lattice impeller lies between the unfilled impeller and the solid impeller. The results show that the impeller with lattice filling hub can not only reduce the weight effectively, but also improve the efficiency of the compressor.
Observation of broad-band water waveguiding in shallow water: a revival
Authors: Sepúlveda-Soto Fabián , Guzmán-Silva Diego , Rosas Edgardo , Vicencio Rodrigo A. , Falcón Claudio 
Published: October 2020

AbstractWe report on the observation and characterization of broad-band waveguiding of surface gravity waves in an open channel, in the shallow water limit. The waveguide is constructed by changing locally the depth of the fluid layer, which creates conditions for surface waves to propagate along the generated guide. We present experimental and numerical results of this shallow water waveguiding, which can be straightforwardly matched to the one-dimensional water wave equation of shallow water waves. Our work revitalizes water waveguiding research as a relevant and controllable experimental setup to study complex phenomena using waveguide geometries.
Additive effect of atropine eye drops and short-term retinal defocus on choroidal thickness in children with myopia
Authors: Chiang Samuel T.-H. , Turnbull Philip R. K. , Phillips John R. 
Published: October 2020

AbstractAtropine eye drops and myopic retinal defocus each slow progression of myopia (short-sight). They also cause thickening of the choroid, and it has been suggested that the thickening is a precursor for reduced eye growth and slowed myopia progression. We investigated whether choroidal thickening due to optical defocus would add to thickening due to atropine when both were applied simultaneously. Addition would suggest that combining the two clinical treatments may improve efficacy of myopia control. We studied 20 children receiving 0.3% atropine daily for myopia control, over a period of 6 months. We imposed short periods of retinal defocus (1 h of myopic or hyperopic defocus (± 2.00D)) both before, and after 1 week and 3 and 6 months of atropine treatment. Prior to atropine, myopic or hyperopic defocus caused significantly thicker or thinner choroids respectively (± 12 µm, p < 0.001). After one week of atropine alone, thickness had increased (+ 21 µm; SD 17 µm; p < 0.001), and it increased further (by + 13 µm; SD 6 µm; p < 0.001) when exposed to myopic defocus. Atropine abolished choroidal thinning in response to hyperopic defocus. These effects remained the same after 3 and 6 months of atropine treatment. Our results show that additive effects of atropine and optical defocus are present at the level of the choroid, and suggest that combining optical and pharmaceutical treatments is likely to enhance efficacy of clinical myopia control.
Superfluid density from magnetic penetration depth measurements in Nb–Cu 3D nano-composite films
Authors: Gupta Chandan , Parab Pradnya , Bose Sangita 
Published: October 2020

AbstractSuperconductivity in 3D Nb–Cu nanocomposite granular films have been studied with varying thickness for two different compositions, Nb rich with 88 at% of Nb and Cu rich with 46 at% of Nb. For both compositions, the superconducting transition temperature (Tc) decreases with decreasing film thickness. For any thickness, doubling the Cu content in the films decreases the Tc by about 2 K. To explore if phase fluctuations play any role in superconductivity in these 3D films, the superfluid stiffness (JS) of the films was measured using low frequency two-coil mutual inductance (M) technique. Interestingly, the measurement of M in magnetic fields showed two peaks in the imaginary component of M for both Nb rich and Cu rich films. The two peaks were associated with the pair-breaking effect of the magnetic field on the intra and inter-granular coupling in these films consisting of random network of superconductor (S) and normal metal (N) nano-particles. Furthermore, JS was seen to decrease with decreasing film thickness and increasing Cu content. However, for all films studied JS remained higher than the superconducting energy gap (∆) indicating that phase fluctuations do not play any role in superconductivity in the film thickness and composition range investigated. Our results indicate that an interplay of quantum size effects (QSE) and superconducting proximity effect (SPE) controls the Tc with composition in these 3D nano-composite films.
Fast kinetics of environmentally induced α-synuclein aggregation mediated by structural alteration in NAC region and result in structure dependent cytotoxicity
Authors: Srivastava Tulika , Raj Ritu , Dubey Amit , Kumar Dinesh , Chaturvedi Rajnish K. , Sharma Sandeep K. , Priya Smriti 
Published: October 2020

AbstractAggregation of α-synuclein (α-syn) is associated with the manifestation of various pathogenic synucleinopathies, including Parkinson’s disease attributed to both genetic and environmental stress factors. The initial events triggering α-syn aggregation and disease initiation due to environmental stress factors are still largely unknown. Here, to understand the mechanism of misfolding and aggregation initiation, we induced α-syn aggregation with rotenone, an established chemical inducer of PD like symptoms. We found that rotenone accelerates the formation of structurally distinct oligomers and fibrils that act as templates and increase the formation of conformers capable of spreading to the neighboring neuronal cells. Molecular dynamics simulations and NMR studies revealed the involvement of NAC region and formation of helical conformations resulting in structural variations in oligomers and fibrils. These structural variations affect the cytotoxic potential of oligomers and fibrils, where, the beta sheet rich oligomers and fibrils alter the membrane potential of neuronal cells and lead to early apoptosis. Our results describe the initial mechanistic events in pathogenic protein aggregation, where initial structural alterations in response to external stress factors dictate the toxicity of resulting conformers. This information will further provide insights in the understanding of protein aggregation, disease progression and pathogenesis.
Phosphorylation of Connexin36 near the C-terminus switches binding affinities for PDZ-domain and 14–3–3 proteins in vitro
Authors: Tetenborg Stephan , Wang Helen Y. , Nemitz Lena , Depping Anne , Espejo Alexsandra B. , Aseervatham Jaya , Bedford Mark T. , Janssen-Bienhold Ulrike , O’Brien John , Dedek Karin 
Published: October 2020

AbstractConnexin36 (Cx36) is the most abundant connexin in central nervous system neurons. It forms gap junction channels that act as electrical synapses. Similar to chemical synapses, Cx36-containing gap junctions undergo activity-dependent plasticity and complex regulation. Cx36 gap junctions represent multimolecular complexes and contain cytoskeletal, regulatory and scaffolding proteins, which regulate channel conductance, assembly and turnover. The amino acid sequence of mammalian Cx36 harbors a phosphorylation site for the Ca2+/calmodulin-dependent kinase II at serine 315. This regulatory site is homologous to the serine 298 in perch Cx35 and in close vicinity to a PDZ binding domain at the very C-terminal end of the protein. We hypothesized that this phosphorylation site may serve as a molecular switch, influencing the affinity of the PDZ binding domain for its binding partners. Protein microarray and pulldown experiments revealed that this is indeed the case: phosphorylation of serine 298 decreased the binding affinity for MUPP1, a known scaffolding partner of connexin36, and increased the binding affinity for two different 14–3–3 proteins. Although we did not find the same effect in cell culture experiments, our data suggest that phosphorylation of serine 315/298 may serve to recruit different proteins to connexin36/35-containing gap junctions in an activity-dependent manner.