bluuebunny/crossref_metadata_2025_split huggingface dataset:
No effect of a dopaminergic modulation fMRI task by amisulpride and L-DOPA on reward anticipation in healthy volunteers
Authors: Grimm Oliver , Nägele Magdalena , Küpper-Tetzel Lea , de Greck Moritz , Plichta Michael , Reif Andreas 
Published: November 2020

Abstract
Rationale
Dysregulation of dopaminergic neurotransmission, specifically altered reward processing assessed via the reward anticipation in the MID task, plays a central role in the etiopathogenesis of neuropsychiatric disorders.

Objectives
We hypothesized to find a difference in the activity level of the reward system (measured by the proxy reward anticipation) under drug administration versus placebo, in that amisulpride reduces, and L-DOPA enhances, its activity.

Methods
We studied the influence of dopamine agonist L-DOPA and the antagonist amisulpride on the reward system using functional magnetic resonance imaging (fMRI) during a monetary incentive delay (MID) task in n = 45 healthy volunteers in a randomized, blinded, cross-over study.

Results
The MID paradigm elicits strong activation in reward-dependent structures (such as ventral striatum, putamen, caudate, anterior insula) during reward anticipation. The placebo effect demonstrated the expected significant blood oxygen level–dependent activity in reward-dependent brain regions. Neither amisulpride nor L-DOPA led to significant changes in comparison with the placebo condition. This was true for whole-brain analysis as well as analysis of a pre-defined nucleus accumbens region-of-interest mask.

Conclusion
The present results cast doubt on the sensitivity of reward anticipation contrast in the MID task for assessing dopamine-specific changes in healthy volunteers by pharmaco-fMRI. While our task was not well-suited for detailed analysis of the outcome phase, we provide reasonable arguments that the lack of effect in the anticipation phase is not due to an inefficient task but points to unexpected behavior of the reward system during pharmacological challenge. Group differences of reward anticipation should therefore not be seen as simple representatives of dopaminergic states.
The future of self-selecting and stable fermentations
Authors: Rugbjerg Peter , Olsson Lisbeth 
Published: November 2020

Abstract
Unfavorable cell heterogeneity is a frequent risk during bioprocess scale-up and characterized by rising frequencies of low-producing cells. Low-producing cells emerge by both non-genetic and genetic variation and will enrich due to their higher specific growth rate during the extended number of cell divisions of large-scale bioproduction. Here, we discuss recent strategies for synthetic stabilization of fermentation populations and argue for their application to make cell factory designs that better suit industrial needs. Genotype-directed strategies leverage DNA-sequencing data to inform strain design. Self-selecting phenotype-directed strategies couple high production with cell proliferation, either by redirected metabolic pathways or synthetic product biosensing to enrich for high-performing cell variants. Evaluating production stability early in new cell factory projects will guide heterogeneity-reducing design choices. As good initial metrics, we propose production half-life from standardized serial-passage stability screens and production load, quantified as production-associated percent-wise growth rate reduction. Incorporating more stable genetic designs will greatly increase scalability of future cell factories through sustaining a high-production phenotype and enabling stable long-term production.
Diel vertical migration of copepods and its environmental drivers in subtropical Bahamian blue holes
Authors: Sha Yongcui , Zhang Huan , Lee Marcus , Björnerås Caroline , Škerlep Martin , Gollnisch Raphael , Herzog Simon David , Ekelund Ugge Gustaf , Vinterstare Jerker , Hu Nan , Pärssinen Varpu , Hulthén Kaj , Nilsson P. Anders , Rengefors Karin , Brönmark Christer , Langerhans R. Brian , Hansson Lars-Anders 
Published: November 2020

AbstractDiel vertical migration (DVM) is the most common behavioral phenomenon in zooplankton, and numerous studies have evaluated DVM under strong seasonality at higher latitudes. Yet, our understanding of the environmental drivers of DVM at low latitudes, where seasonal variation is less pronounced, remains limited. Therefore, we here examined patterns of vertical distribution in copepods in six subtropical Bahamian blue holes with different food web structure and tested the role of several key environmental variables potentially affecting this behavior. Day and night samplings showed that copepods generally performed DVM, characterized by downward migration to deeper depths during the day and upward migration to surface waters at night. Across all blue holes, the daytime vertical depth distribution of calanoid copepods correlated positively with both predation risk and depth of food resources (Chlorophylla), but was less affected by ultraviolet radiation (UVR). A potential explanation is that since UVR is a continuous threat across seasons, zooplankton have established photoprotective pigmentation making them less vulnerable to this threat. The copepods also showed a size-structured depth segregation, where larger individuals were found at deeper depths during the day, which further strengthens the suggestion that predation is a major driver of DVM in these systems. Hence, in contrast to studies performed at higher latitudes, we show that despite the constant exposure to UVR, predator avoidance and food availability are the most pronounced drivers of copepod DVM at those low latitudes, suggesting that the main driver of DVM may vary among systems, but also systematically by latitude.
High Glucose and Hypoxia-Mediated Damage to Human Brain Microvessel Endothelial Cells Induces an Altered, Pro-Inflammatory Phenotype in BV-2 Microglia In Vitro
Authors: Iannucci Jaclyn , Rao Haripriya Vittal , Grammas Paula 
Published: November 2020

AbstractDiabetes is strongly linked to the development of Alzheimer’s disease (AD), though the mechanisms for this enhanced risk are unclear. Because vascular inflammation is a consistent feature of both diabetes and AD, the cerebral microcirculation could be a key target for the effects of diabetes in the brain. The goal of this study is to explore whether brain endothelial cells, injured by diabetes-related insults, glucose and hypoxia, can affect inflammatory and activation processes in microglia in vitro. Human brain microvascular endothelial cells (HBMVECs) were either treated with 5 mM glucose (control), 30 mM glucose (high glucose), exposed to hypoxia, or exposed to hypoxia plus high glucose. HBMVEC-conditioned medium was then used to treat BV-2 microglia. Alterations in microglia phenotype were assessed through measurement of nitric oxide (NO), cytokine production, microglial activation state markers, and microglial phagocytosis. HBMVECs were injured by exposure to glucose and/or hypoxia, as assessed by release of LDH, interleukin (IL)-1β, and reactive oxygen species (ROS). HBMVECs injured by glucose and hypoxia induced increases in microglial production of NO, tumor necrosis factor-α (TNFα) and matrix metalloproteinase (MMP)-9. Injured HBMVECs significantly increased microglial expression of CD11c and CLEC7A, and decreased expression of the homeostatic marker P2RY12. Finally, bead uptake by BV-2 cells, an index of phagocytic ability, was elevated by conditioned media from injured HBMVECs. The demonstration that injury to brain endothelial cells by diabetic-associated insults, glucose and hypoxia, promotes microglial inflammation supports the idea that the cerebral microcirculation is a critical locus for the deleterious effects of diabetes in the AD brain.
Supervisor synthesis and throughput optimization of partially-controllable manufacturing systems
Authors: van Putten Berend Jan Christiaan , van der Sanden Bram , Reniers Michel , Voeten Jeroen , Schiffelers Ramon 
Published: November 2020

AbstractOne of the challenges in the design of supervisors with optimal throughput for manufacturing systems is the presence of behavior outside the control of the supervisor. Uncontrollable behavior is typically encountered in the presence of (user) inputs, external disturbances, and exceptional behavior. This paper introduces an approach for the modeling and synthesis of a throughput-optimal supervisor for manufacturing systems with partially-controllable behavior on two abstraction levels. Extended finite automata are used to model the high abstraction level in terms of system activities, where uncontrollability is modeled by the presence of uncontrollable activities. In the lower abstraction level, activities are modeled as directed acyclic graphs that define the constituent actions and dependencies between them. System feedback from the lower abstraction level, including timing, is captured using variables in the extended finite automata of the higher abstraction level. For throughput optimization, game-theoretic methods are employed on the state space of the synthesized supervisor to determine a guarantee to the lower-bound system performance. This result is also used in a new method to automatically compute a throughput-optimal controller that is robust to the uncontrollable behavior.
Future proofing the principle of no significant harm
Authors: Gupta Joyeeta , Schmeier Susanne 
Published: November 2020

AbstractThe principle of ‘no significant harm’ as a way of addressing transboundary environmental challenges is both inadequately researched and inadequately implemented in many parts of the world. This paper addresses the questions: What is the nature of transboundary harm in the Anthropocene? Is the principle of no significant harm able to address current and pre-empt future transboundary harm in the field of water and environmental law? This special issue has focused on this principle in the arena of water law. This article integrates the findings in the context of a broader understanding of global harm in the Anthropocene. We draw 4 conclusions. First, conceptually harm is moving beyond direct inter-state harm between neighbouring countries to a multi-directional, multi-actor/multi-level harm, which is increasingly creeping and cumulative, with growing spatial and temporal characteristics. It thus requires moving beyond quibbling over what is ‘significant’ harm to recognize the climate emergency, the sixth biodiversity extinction, the huge damage to water systems and to realize that the threshold of ecosystem and human tolerance of damage are reducing rapidly. Second, however, the no-harm principle tends to be better developed in qualifying sovereignty in relation to transboundary harm on rivers than in the broader environmental and development arena as demonstrated by agenda 2030 which reverts to full permanent sovereignty. Third, legal scholarship, however, does provide a wide range of instruments for addressing harm before it occurs, after it has happened, and considering the differentiated economic capacity of the actors. Finally, the larger problem is that it is not individual projects or programmes that cause problems as much as national prioritization of economic growth which has led to externalizing the environment. The no-harm principle will be ineffective if it cannot be used to question the content of ‘growth’-led policies. There is need to future proof the no-harm principle.
CZTS thin film solar cells on flexible Molybdenum foil by electrodeposition-annealing route
Authors: Khalil M. I. , Bernasconi R. , Lucotti A. , Le Donne A. , Mereu R. A. , Binetti S. , Hart J. L. , Taheri M. L. , Nobili L. , Magagnin L. 
Published: November 2020

Abstract
Earth-abundant and non-toxic Kesterite-based Cu2ZnSnS4 (CZTS) thin film solar cells are successfully fabricated on flexible Molybdenum (Mo) foil substrates by an electrodeposition-annealing route. A well-adherent, densely packed, homogeneous, compact, and mirror-like CZT precursor is initially produced through electrodeposition by using a rotating working electrode. Subsequently, the co-electrodeposited CuZnSn (CZT) precursor is sulfurized in quartz tube furnace at 550 °C for 2 h in N2 atmosphere with the presence of elemental sulfur in order to form CZTS. Different characterization techniques like XRD, SEM, HR-TEM, Raman, and Photoluminescence demonstrate that almost phase-pure CZTS formed after sulfurization. A flexible Al/Al-ZnO/i-ZnO/CdS/CZTS/Mo foil solar cell is produced, where CdS is deposited by chemical bath deposition and transparent conducting oxide (TCO) is deposited by DC sputtering. The CZTS solar device shows a 0.55% power conversion efficiency on flexible Mo foil substrate and it constitutes the first prototype of this kind of solar cell produced by electrodeposition-annealing route without any surface modification of the Mo substrate.

Graphic abstract
Locust bean gum as green and water-soluble binder for LiFePO4 and Li4Ti5O12 electrodes
Authors: Jakóbczyk Paweł , Bartmański Michał , Rudnicka Ewelina 
Published: November 2020

Abstract
Locust Bean Gum (LBG, carob bean gum) was investigated as an environmentally friendly, natural, and water-soluble binder for cathode (LFP) and anode (LTO) in lithium-ion batteries (Li-ion). For the first time, we show LBG as an electrode binder and compare to those of the most popular aqueous (CMC) and conventional (PVDF) binders. The electrodes were characterized using TGA/DSC, the galvanostatic charge–discharge cycle test, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Thermal decomposition of LBG is seen to begin above 250 °C with a weight loss of about 60 wt% observed at 300 °C, which is sufficient to ensure stable performance of the electrode in a Li-ion battery. For CMC, weight loss at the same temperature is about 45%. Scanning electron microscopy (SEM) shows that the LFP–LBG system has a similar distribution of conductive carbon black particles to PVDF electrodes. The LTO–LBG electrode has a homogeneous dispersion of the electrode elements and maintains the electrical integrity of the network even after cycling, which leads to fast electron migration between LTO and carbon black particles, as well as ion conductivity between LTO active material and electrolyte, better than in systems with CMC and PVDF. The exchange current density, obtained from impedance spectroscopy fell within a broad range between 10−4 and 10−2 mA cm−2 for the LTO|Li and LFP|Li systems, respectively. The results presented in this paper indicate that LBG is a new promising material to serve as a binder.

Graphic abstract
Family-Centered Care: How Close Do We Get When Talking to Parents of Children Undergoing Diagnosis for Autism Spectrum Disorders?
Authors: Myers Lynnea , Karp Sharon M. , Dietrich Mary S. , Looman Wendy S. , Lutenbacher Melanie 
Published: November 2020

AbstractAutism spectrum disorder (ASD) affects 1:59 children, yet little is known about parents’ perceptions of family-centered care (FCC) during the diagnostic process leading up to diagnosis. This mixed-methods study explored key elements of FCC from 31 parents of children recently diagnosed with ASD using parallel qualitative and quantitative measures. Parents rated highly their receipt of FCC and discussed ways providers demonstrated FCC. However, the majority of parents indicated that the period when their child was undergoing diagnosis was stressful and reported symptoms of depression and anxiety. The study points to ways in which health care providers can enhance FCC provided to families when a child is undergoing ASD diagnosis.
Risk for Premature Mortality and Intentional Self-harm in Autism Spectrum Disorders
Authors: Jokiranta-Olkoniemi Elina , Gyllenberg David , Sucksdorff Dan , Suominen Auli , Kronström Kim , Chudal Roshan , Sourander Andre 
Published: November 2020

AbstractTo examine the risk for premature mortality and intentional self-harm in autism spectrum disorders (ASD). Based on a national birth cohort. Children born in 1987–2005, diagnosed with ASD by 2007 (n = 4695) were matched with four non-ASD subjects (n = 18,450) and followed until 2015 for mortality and intentional self-harm. The risk among ASD subjects was elevated only for natural cause of death. The risk for intentional self-harm was increased in the unadjusted analyses, but decreased to non-significant after adjusting for comorbid psychiatric disorders. ASD subjects are at increased risk for premature mortality due to natural causes of death. While ASD subjects die of suicide and present with more self-harm, the association is explained by comorbid psychiatric disorders.
Molecular chaperones and their denaturing effect on client proteins
Authors: Hiller Sebastian 
Published: November 2020

AbstractAdvanced NMR methods combined with biophysical techniques have recently provided unprecedented insight into structure and dynamics of molecular chaperones and their interaction with client proteins. These studies showed that several molecular chaperones are able to dissolve aggregation-prone polypeptides in aqueous solution. Furthermore, chaperone-bound clients often feature fluid-like backbone dynamics and chaperones have a denaturing effect on clients. Interestingly, these effects that chaperones have on client proteins resemble the effects of known chaotropic substances. Following this analogy, chaotropicity could be a fruitful concept to describe, quantify and rationalize molecular chaperone function. In addition, the observations raise the possibility that at least some molecular chaperones might share functional similarities with chaotropes. We discuss these concepts and outline future research in this direction.
Translation, cultural adaptation and construct validity of the German version of the Adult Social Care Outcomes Toolkit for informal Carers (German ASCOT-Carer)
Authors: Trukeschitz Birgit , Hajji Assma , Litschauer Judith , Malley Juliette , Schoch Adiam , Rand Stacey , Linnosmaa Ismo , Forder Julien 
Published: November 2020

AbstractPurposeThe Adult Social Care Outcomes Toolkit for Carers (ASCOT-Carer), developed in England, measures the effects of long-term care (LTC) services and carer support on informal carers’ quality of life (QoL). Translations of the ASCOT-Carer into other languages are useful for national and cross-national studies. The aim of this paper was to report on the translation and cultural adaptation of the original English ASCOT-Carer into German, to assess its content validity and to test for its construct validity (convergent and discriminative/known-group validity).MethodsTranslation and cultural adaptation followed the ISPOR TCA guidelines. As part of the translation and adaptation process, five cognitive debriefing interviews with informal carers were used for evaluating linguistic and content validity. In addition, a sample of 344 informal carers of older adults, who received home care services in Austria, was used for hypothesis testing as suggested by the COSMIN checklist to assess convergent and discriminative/known-group validity as part of construct validity.ResultsCognitive interviews provided evidence that questions and response options of the German ASCOT-Carer were understood as intended. Associations between ASCOT-Carer scores/domains and related outcome measures (convergent validity) and expected groups of informal carers and the care service users they care for (discriminative validity) supported construct validity of the translated instrument.ConclusionThe German ASCOT-Carer instrument meets the required standards for content and construct validity which supports its usefulness for (cross-)national studies on LTC-service-related QoL-outcomes in informal carers. Research is encouraged to assess further measurement properties of the translated instrument.
Oxidation of glucose and arabinose mixtures over Au/Al2O3
Authors: Franz Sebastian , Shcherban Nataliya D. , Simakova Irina L. , Peurla Markus , Eränen Kari , Wärnå Johan , Salmi Tapio , Murzin Dmitry Yu. 
Published: November 2020

AbstractOxidation of a mixture of glucose and arabinose over 1% Au deposited on alumina was investigated in a semi-batch reactor varying pH, temperature and partial pressure of oxygen. Elevation of the latter enhancing the rate induced also losses in selectivity to aldonic acids. A kinetic model representing the catalytic oxidation reactions of arabinose and glucose along with respective isomerization to fructose and ribulose was developed. Calculations based on the model were able to describe experimental data in a reliable way.
Procurement policy and SME participation in public purchasing
Authors: Hoekman Bernard , Taş Bedri Kamil Onur 
Published: November 2020

AbstractThis study investigates the relationship between regulatory policies governing public procurement and participation by small and medium enterprises (SMEs), using a large dataset on European procurement. We find that better quality procurement regulation is associated with greater SME participation and higher probability that SMEs win contracts. Dividing contracts into smaller lots, a key feature of 2014 EU procurement regulation reform, bolsters participation by SMEs but only increases the probability of SMEs winning contracts for small value lots (€25,000 or less). Our results suggest governments seeking to enhance participation by SMEs in public procurement without explicitly favoring SMEs can do so by improving the overall quality of procurement processes.
Bleeding risk in patients with venous thromboembolic events treated with new oral anticoagulants
Authors: Wallvik Niklas , Renlund Henrik , Själander Anders 
Published: November 2020

AbstractNew oral anticoagulants (NOACs) is the preferred treatment in secondary prophylaxis of venous thromboembolic events (VTE). The aim of this study was to investigate possible risk factors associated with major bleeding in VTE-patients treated with NOACs. In this retrospective register-based study we screened the Swedish anticoagulation register Auricula (during 2012.01.01–2017.12.31) to find patients and used other national registers for outcomes. Primary endpoint was major bleeding defined as bleeding leading to hospital care. Multivariate Cox-regression analysis was used to reveal risk factors. 18 219 patients with NOAC due to VTE were included. 85.6% had their first VTE, mean age was 69.4 years and median follow-up time was 183 days. The most common NOAC was rivaroxaban (54.8%), followed by apixaban (42.0%), dabigatran (3.2%) and edoxaban (0.1%). The rate of major bleeding was 6.62 (95% CI 6.19–7.06) per 100 treatment years in all patients and 11.27 (CI 9.96–12.57) in patients above 80 years of age. Statistically independent risk factors associated with major bleeding were age (normalized HR 1.38, CI 1.27–1.50), earlier major bleeding (HR 1.58, Cl 1.09–2.30), COPD (HR 1.28, CI 1.04–1.60) and previous stroke (HR 1.28, Cl 1.03–1.58) or transient ischemic attack (TIA) (HR 1.33, Cl 1.01–1.76). Prior warfarin treatment was protective (HR 0.67, CI 0.58–0.78). This real world cohort shows a high bleeding rate especially among the elderly and in patients with previous major bleeding, COPD and previous stroke or TIA. This should be considered when deciding on treatment duration and NOAC dose in these patients.