bluuebunny/crossref_metadata_2025_split huggingface dataset:
Species specific gene expression dynamics during harmful algal blooms
Authors: Metegnier Gabriel , Paulino Sauvann , Ramond Pierre , Siano Raffaele , Sourisseau Marc , Destombe Christophe , Le Gac Mickael 
Published: April 2020

AbstractHarmful algal blooms are caused by specific members of microbial communities. Understanding the dynamics of these events requires comparing the strategies developed by the problematic species to cope with environmental fluctuations to the ones developed by the other members of the community. During three consecutive years, the meta-transcriptome of micro-eukaryote communities was sequenced during blooms of the toxic dinoflagellate Alexandrium minutum. The dataset was analyzed to investigate species specific gene expression dynamics. Major shifts in gene expression were explained by the succession of different species within the community. Although expression patterns were strongly correlated with fluctuation of the abiotic environment, and more specifically with nutrient concentration, transcripts specifically involved in nutrient uptake and metabolism did not display extensive changes in gene expression. Compared to the other members of the community, A. minutum displayed a very specific expression pattern, with lower expression of photosynthesis transcripts and central metabolism genes (TCA cycle, glucose metabolism, glycolysis…) and contrasting expression pattern of ion transporters across environmental conditions. These results suggest the importance of mixotrophy, cell motility and cell-to-cell interactions during A. minutum blooms.
Efficient Biocatalytic Preparation of Rebaudioside KA: Highly Selective Glycosylation Coupled with UDPG Regeneration
Authors: Zhang Yunyun , Xu Shaohua , Jin Yue , Dai Yan , Chen Yijun , Wu Xuri 
Published: April 2020

AbstractRebaudioside KA is a diterpene natural sweetener isolated in a trace amount from the leaves of Stevia rebaudiana. Selective glycosylation of rubusoside, a natural product abundantly presented in various plants, is a feasible approach for the biosynthesis of rebaudioside KA. In this study, bacterial glycosyltransferase OleD was identified to selectively transfer glucose from UDPG to 2′-hydroxyl group with a β-1,2 linkage at 19-COO-β-D-glucosyl moiety of rubusoside for the biosynthesis of rebaudioside KA. To eliminate the use of UDPG and improve the productivity, a UDPG regeneration system was constructed as an engineered Escherichia coli strain to couple with the glycosyltransferase. Finally, rubusoside at 22.5 g/L (35.0 mM) was completely converted to rebaudioside KA by the whole cells without exogenous addition of UDPG. This study provides an efficient and scalable method for highly selective biosynthesis of rebaudioside KA.
Atemstörungen im Schlaf
Authors: Möller A. 
Published: April 2020

ZusammenfassungAtemstörungen im Schlaf haben bei Kindern eine hohe klinische Relevanz. Sie betreffen nicht nur einen großen zeitlichen Anteil im Leben der Kinder, sondern haben auch negative Auswirkungen auf das Gedeihen, die kardiovaskuläre Funktion und die kognitive Entwicklung. Verschiedene entwicklungsspezifische Faktoren müssen in der Beurteilung von Schlafuntersuchungen bei Kindern berücksichtigt werden. Die Adeno-Tonsillenhyperplasie ist sicherlich der häufigste Grund für eine Atemstörung im Schlaf, aber das Spektrum an Ursachen ist sehr breit. Gerade syndromale kranio-faziale Missbildungen und metabolische Erkrankungen sind mit einem sehr hohen Risiko einer relevanten Atemstörung assoziiert. Eine korrekte und kindergerechte Diagnostik ist essenziell, um eine adäquate Therapie zu ermöglichen.
Großer Pneumothorax unter CPAP-Therapie beim Schlafapnoepatienten ohne vorbekannte Lungen- und Thoraxerkrankungen – ein Fallbericht
Authors: Langner S. , Kolditz M. , Kleymann J. , Tausche K. , Almeida A. B. , Schweigert M. , Koschel D. 
Published: April 2020

ZusammenfassungEine CPAP-Therapie ist die häufigste Behandlungsform der obstruktiven Schlafapnoe.Schwerwiegende Komplikation dieser Behandlung sind sehr selten. Pneumothorax als Lungen-Barotrauma unter der CPAP-Therapie wird in Einzelfallberichten und ausschließlich bei vorbestehenden Lungen- und Thoraxerkrankungen beschrieben.Ein 68-jähriger, pulmonal nicht vorerkrankter Schlafapnoe-Patient mit einer langjährig etablierten CPAP-Therapie wird nach einem heftigen thorakalen Schmerzereignis mit anhaltender Luftnotsymptomatik stationär aufgenommen. Radiologisch und computertomografisch bestand ein ausgedehnter rechtsseitiger Pneumothorax mit rechts basal betontem bullösem Emphysem. Nach operativer Versorgung des sekundären Spontanpneumothorax konnte am 3. postoperativen Tag die nächtliche Überdruckatmung unter reduziertem CPAP-Druck mit zufriedenstellendem Schlafapnoe-Befund und ohne Pneumothorax-Rezidiv im Verlauf wiederaufgenommen werden.Als mögliche Pneumothorax-Ursache bei dem Patienten können alveoläre entzündliche Veränderungen infolge von Überdehnung und Drucksteigerung in Alveolen, die unter jahrelanger CPAP-Behandlung mit schrittweiser Drucksteigerung entstanden sind und folglich zu ausgedehnten Lungenveränderung mit Ruptur geführt haben, angenommen werden.Zusammenfassend und schlussfolgernd soll bei Patienten, die aufgrund einer Schlafapnoe über Jahre mittels Überdruckatmung behandelt werden, bei plötzlich einsetzenden thorakalen Schmerzen mit einer Luftnotsymptomatik an einen möglichen Spontanpneumothorax gedacht werden.
Effects of Homoeopathic Single and Minimum Dose on Non-Radiographic Axial Spondyloarthritis—BASDAI Assessment
Authors: Kumar Deepak , Suchiang Eiphrangdaka L. , Pal Gautam 
Published: April 2020

AbstractEarly cases of ankylosing spondylitis do not have the typical presentation of radiographic sacroiliitis but present as non-radiographic axial spondyloarthritis (nr-axSpA) where both are varieties of axSpA. Homoeopathic prescription based on totality of symptoms offers a promising relief to nr-axSpA where peripheral joints are also affected. Here, a 27-year-old male patient presented with bilateral heel pain, pain in low back, pain on base of right toes, pain in neck with stiffness for 1 year had refractive response to conventional medication. Diagnosis was confirmed by Assessment of SpondyloArthritis International Society diagnostic criteria for axSpA. Single medicine and minimum dose of Silicea showed its effectiveness on the symptoms' improvement and Bath Ankylosing Spondylitis Disease Activity Index score whereby score of 8.8 (active disease) before treatment changed to 1.2 (inactive or mild disease) after treatment. Various other parameters were assessed accordingly before and after treatment. This case report encourages further exploring the beneficial effects of homoeopathic treatment in clinical condition like axSpA utilising validated scale.
Corticosteroid Guidance for Pregnancy during COVID-19 Pandemic
Authors: McIntosh Jennifer Jury 
Published: April 2020

The novel coronavirus disease 2019 (COVID-19) pandemic is causing a necessary, rapid adjustment within the field of obstetrics. Corticosteroid use is a mainstay of therapy for those women delivering prematurely. Unfortunately, corticosteroid use has been associated with worse outcomes in COVID-19 positive patients. Given this information, it is necessary that obstetricians adjust practice to carefully weigh the fetal benefits with maternal risks. Therefore, our institution has examined the risks and benefits and altered our corticosteroid recommendations.
Key Points
Gender-wise Long-term Predictors for Major Adverse Cardiac Events Following Percutaneous Coronary Intervention in the Elderly Population
Authors: Ramakrishna A. Siva , Rao V. Satish Kumar , Indrani Garre 
Published: April 2020

Abstract
Background We aimed to recognize the predictors of long-term major adverse cardiac events (MACE) in the elderly candidates for elective percutaneous coronary intervention (PCI) in relation to gender at our center.
Methods In this retrospective cohort study, we reviewed the data of the elderly patients (age ≥70 years) who underwent elective PCI who met our study criteria in our institution during 2008 and 2018. Demographical data, clinical history, angiographic details, PCI procedure, and follow-up data of the patients enrolled in the study were studied by using the angiographic and PCI procedure details. Patients were characterized in the study group as those with or without MACE, which were then compared and analyzed using the statistical analysis in a univariable and binary linear regression analysis.
Results A total of 355 elderly patients (older than 70 years) undergoing elective PCI were selected who fulfilled the inclusion criteria; 277 patients were men and had more comorbidities, including hypertension, diabetes mellitus (DM), chronic obstructive pulmonary disease, history of heart failure, previous coronary artery bypass graft, and presentation with acute coronary syndrome. MACE occurred in 24 events patients of whom 20 were suffering from DM. Binary logistic regression showed that the only determinant for the 1-year follow-up outcome is diabetes (p = 0.000). Even in univariate analysis, DM (0.01) is the determinant. DM is a strong predictor for death in univariate analysis (p = 0.00).
Conclusion PCI is a safe and effective method of coronary revascularization in elderly patients, and some risk factors can predict long-term MACE in this group of patients.
La evolución de las marcas ortográficas y tipográficas en los procesos de lexicalización de neologismos
Authors: Renau Irene , Nazar Rogelio , Lecaros Valesca 
Published: April 2020

Resumen
Presentamos un estudio correlacional de la asociación entre el uso de marcas ortotipográficas y la neologicidad de
un término en un corpus de prensa diacrónico. La pregunta es si es posible observar empíricamente un patrón de evolución en el
porcentaje de veces que una palabra nueva es marcada con comillas o cursiva entre otras y si las marcas declinan conforme la
palabra se asimila al sistema lingüístico. Estudiamos una muestra de 40 neologismos del ámbito de la economía utilizados en prensa
en el periodo 2006–2016, que incluye la crisis económica española. Los resultados, obtenidos a partir del análisis de 41.695
contextos de uso, indican una tendencia descendente de las marcas de los neologismos en el periodo estudiado que, sin embargo, no
es estadísticamente significativa. Junto con ello, no todos los tipos de neologismos se comportan de la misma manera, ya que unos
están más asimilados que otros.
Supresión y adición en textos literarios a la luz del corpus COVALT
Authors: Guzmán Pitarch Josep 
Published: April 2020

Resumen
La omisión y la adición son conceptos definidos en diversos trabajos. Sin embargo, estos estudios se han centrado en una pequeña serie de ejemplos, sin tener en cuenta el alcance del fenómeno. Este trabajo analiza la omisión o adición de segmentos textuales incluidos en un corpus, COVALT, formado por traducciones y originales de textos literarios, cuyo idioma de origen es el alemán y el idioma de destino es el catalán.
Los hallazgos del estudio muestran que todos los textos analizados omiten algún segmento, de modo que podríamos inferir que la omisión está mucho más generalizada de lo que se podría pensar. Además, aunque todos los textos tienen omisiones, no todos tienen adiciones. Así, también podemos concluir que la omisión es más usual que la adición. Del mismo modo, no parece haber correlación significativa entre omisiones y adiciones. Finalmente, nuestro análisis parece señalar que los criterios por los cuales los traductores usan estas técnicas son generalmente más justificables en el caso de la omisión que en el de la adición.
Análisis de las pautas para la enseñanza del inglés en Educación Infantil mediante la técnica Deplhi
Authors: Andúgar Ana , Cortina-Pérez Beatriz , Tornel Abellán María 
Published: April 2020

Resumen
El Consejo de Europa ha impulsado la introducción de lenguas en etapas muy tempranas con la intención de conformar
una Europa plurilingüe. En España, la enseñanza del inglés como lengua extranjera (LE) se inicia habitualmente en el segundo ciclo
de Infantil (3–6 años), aunque hay una falta de acuerdo sobre cómo esta introducción temprana debe realizarse. Esta investigación
tiene como objetivo la búsqueda de consenso sobre las pautas más acertadas para enseñar inglés como LE en Infantil. Se ha
realizado un estudio cualitativo utilizando la técnica Delphi en el que 99 expertos a nivel nacional han tenido que posicionarse
en relación con las pautas didácticas que llevarían a conseguir el éxito en este tipo de programas. Las conclusiones sugieren que
los expertos abogan por una enseñanza de la LE similar al aprendizaje de la lengua materna, creando un contexto de aprendizaje lo
más natural y lúdico posible.
“Me vais a permitir que me pongaen planprofesor”
Authors: Rodríguez-Abruñeiras Paula 
Published: April 2020

ResumenA partir de un estudio de corpus, en este trabajo se analiza el empleo de la locuciónen plan (de)desde una perspectiva funcional. En términos generales, se distinguen un total de siete usos distintos, los cuales pueden agruparse en dos macrofunciones: la adverbial y la discursiva. Los usos discursivos tan solo se registran desde finales del siglo XX, y aparecen de forma más recurrente en textos orales. El estudio también muestra la variación existente entre la locución prepositivaen plan dey la adverbialen plan, la cual gana terreno en los textos más recientes. Por último, se ofrece un análisis detallado del uso deen plan (de)en el habla de los adolescentes españoles de comienzos del siglo XXI, pues es en este sector demográfico donde la fórmula tiene una frecuencia más elevada.
Algoritmos fonéticos para la detección de palabras fonéticamente similares en el español del centro de México
Authors: Hernández-Mena Carlos Daniel , Meza-Ruiz Ivan V. , Ramírez-Orta Juan Antonio , Herrera-Camacho José Abel 
Published: April 2020

ResumenEn la actualidad, la detección de palabras fonéticamente similares se ha logrado de forma exitosa gracias a la utilización de algoritmos fonéticos. Sin embargo, tales algoritmos dependen del lenguaje al que pertenecen, por lo que generalmente no están optimizados para el español. Por esta razón, en el siguiente artículo se presentará el algoritmo PFS y su variante PFS-US, los cuales son algoritmos fonéticos que consideran la fonología del español hablado en el centro de México, y fueron diseñados para detectar palabras fonéticamente similares en grandes conjuntos de palabras. Ahora bien, a través de un análisis comparativo entre otros cuatro algoritmos fonéticos de estado del arte, analizaremos la consideración fonológica mencionada. Para ello, se definieron métricas independientes de la lengua para evaluar algoritmos fonéticos en general. Dichas métricas se basan en la estructura de los grupos de palabras fonéticamente similares entre sí y su relación con palabras que no son similares con ninguna otra. Adicionalmente, los recursos generados se comparten de forma libre para su uso y análisis.
Poisson-Commutative Subalgebras of 𝒮(𝔤) associated with Involutions
Authors: Panyushev Dmitri I , Yakimova Oksana S 
Published: April 2020

Abstract
The symmetric algebra ${\mathcal{S}}({{\mathfrak{g}}})$ of a reductive Lie algebra ${{\mathfrak{g}}}$ is equipped with the standard Poisson structure, that is, the Lie–Poisson bracket. Poisson-commutative subalgebras of ${\mathcal{S}}({{\mathfrak{g}}})$ attract a great deal of attention because of their relationship to integrable systems and, more recently, to geometric representation theory. The transcendence degree of a Poisson-commutative subalgebra ${\mathcal C}\subset{\mathcal{S}}({{\mathfrak{g}}})$ is bounded by the “magic number” ${\boldsymbol{b}}({{\mathfrak{g}}})$ of ${{\mathfrak{g}}}$. There are two classical constructions of $\mathcal C$ with ${\textrm{tr.deg}}\,{\mathcal C}={\boldsymbol{b}}({{\mathfrak{g}}})$. The 1st one is applicable to $\mathfrak{gl}_n$ and $\mathfrak{so}_n$ and uses the Gelfand–Tsetlin chains of subalgebras. The 2nd one is known as the “argument shift method” of Mishchenko–Fomenko. We generalise the Gelfand–Tsetlin approach to chains of almost arbitrary symmetric subalgebras. Our method works for all types. Starting from a symmetric decompositions ${{\mathfrak{g}}}={{\mathfrak{g}}}_0\oplus{{\mathfrak{g}}}_1$, Poisson-commutative subalgebras ${{\mathcal{Z}}},\tilde{{\mathcal{Z}}}\subset{\mathcal{S}}({{\mathfrak{g}}})^{{{\mathfrak{g}}}_0}$ of the maximal possible transcendence degree are constructed. If the ${{\mathbb{Z}}}_2$-contraction ${{\mathfrak{g}}}_0\ltimes{{\mathfrak{g}}}_1^{\textsf{ab}}$ has a polynomial ring of symmetric invariants, then $\tilde{{\mathcal{Z}}}$ is a polynomial maximal Poisson-commutative subalgebra of ${\mathcal{S}}({{\mathfrak{g}}})^{{{\mathfrak{g}}}_0}$ and its free generators are explicitly described.
Baseline and multinormal distribution of ex vivo susceptibilities of Plasmodium falciparum to methylene blue in Africa, 2013–18
Authors: Gendrot Mathieu , Madamet Marylin , Mosnier Joel , Fonta Isabelle , Amalvict Rémy , Benoit Nicolas , Briolant Sébastien , Pradines Bruno ,   French National Reference Centre for Imported Malaria Study Group, Augis V , Bastien P , Berry A , Brouqui P , Chauvin P , Cividin M , Courtier F , Delaunay P , Delhaes L , Drancourt M , Dubosc N , Gaillard T , Genin A , Garnotel E , Javelle E , L’Ollivier C , Lagier J C , Ledault E , Leveque M , Malvy D , Marty P , Ménard G , Menu E , Millet P , Minodier P , Parola P , Picot S , Pomares-Estran C , Ranque S , Receveur M-C , Robin A , Sappa E , Savini H , Sevestre J , Simon F , Sterkers Y , Surcouf C , Varlet E , Wolff A 
Published: April 2020

Abstract

Background
Plasmodium falciparum resistance to most antimalarial compounds has emerged in Southeast Asia and spread to Africa. In this context, the development of new antimalarial drugs is urgent.

Objectives
To determine the baseline in vitro activity of methylene blue (Proveblue®) on African isolates and to determine whether parasites have different phenotypes of susceptibility to methylene blue.

Methods
Ex vivo susceptibility to methylene blue was measured for 609 P. falciparum isolates of patients hospitalized in France for malaria imported from Africa. A Bayesian statistical analysis was designed to describe the distribution of median effective concentration (EC50) estimates.

Results
The EC50 ranged from 0.16 to 87.2 nM with a geometric mean of 7.17 nM (95% CI = 6.21–8.13). The 609 EC50 values were categorized into four components: A (mean = 2.5 nM; 95% CI = 2.28–2.72), B (mean = 7.44 nM; 95% CI = 7.07–7.81), C (mean = 16.29 nM; 95% CI = 15.40–17.18) and D (mean = 38.49 nM; 95% CI = 34.14–42.84). The threshold value for in vitro reduced susceptibility to methylene blue was estimated at 35 nM using the geometric mean of EC50 plus 2 SDs of the 609 isolates. This cut-off also corresponds to the lower limit of the 95% CI of the methylene blue EC50 of component D. Thirty-five isolates (5.7%) displayed EC50 values above this threshold.

Conclusions
Methylene blue exerts a promising efficacy against P. falciparum and is a potential partner for triple combinations.
Efferocytosis during myocardial infarction
Authors: Yoshimura Chikashi , Nagasaka Akiomi , Kurose Hitoshi , Nakaya Michio 
Published: April 2020

AbstractMyocardial infarction is one of the major causes of death worldwide. Many heart cells die during myocardial infarction through various processes such as necrosis, apoptosis, necroptosis, autophagy-related cell death, pyroptosis and ferroptosis. These dead cells in infarcted hearts expose the so-called ‘eat-me’ signals, such as phosphatidylserine, on their surfaces, enhancing their removal by professional and non-professional phagocytes. Clearance of dead cells by phagocytes in the diseased hearts plays a crucial role in the pathology of myocardial infarction by inhibiting the inflammatory responses caused by the leakage of contents from dead cells. This review focuses on the rapidly growing understanding of the molecular mechanisms of dead cell phagocytosis, termed efferocytosis, during myocardial infarction, which contributes to the pathophysiology of myocardial infarction.
Clinical and predictive value of simplified creatinine index used as muscle mass surrogate in end-stage kidney disease haemodialysis patients—results from the international MONitoring Dialysis Outcome initiative
Authors: Canaud Bernard , Ye Xiaoling , Usvyat Len , Kooman Jeroen , van der Sande Frank , Raimann Jochen , Wang Yuedong , Kotanko Peter 
Published: April 2020

AbstractBackgroundProtein-energy wasting, muscle mass (MM) loss and sarcopenia are highly prevalent and associated with poor outcome in haemodialysis (HD) patients. Monitoring of MM and/or muscle metabolism in HD patients is of paramount importance for timely detection of muscle loss and to intervene adequately. In this study we assessed the reliability and reproducibility of a simplified creatinine index (SCI) as a surrogate marker of MM and explored its predictive value on outcome.MethodWe included all in-centre HD patients from 16 European countries with at least one SCI. The baseline period was defined as 30 days before and after the first multifrequency bioimpedance spectroscopy measurement; the subsequent 7 years constituted the follow-up. SCI was calculated by the Canaud equation. Multivariate Cox proportional hazards models were applied to assess the association of SCI with all-cause mortality. Using backward analysis, we explored the trends of SCI before death. Bland–Altman analysis was performed to analyse the agreement between estimated and measured MM.ResultsWe included 23 495 HD patients; 3662 were incident. Females and older patients have lower baseline SCI. Higher SCI was associated with a lower risk of mortality [hazard ratio 0.81 (95% confidence interval 0.79–0.82)]. SCI decline accelerated ∼5–7 months before death. Lean tissue index (LTI) estimated by SCI was correlated with measured LTI in both sexes (males: R2 = 0.94; females: R2 = 0.92; both P &lt; 0.001). Bland–Altman analysis showed that measured LTI was 4.71 kg/m2 (±2 SD: −12.54–3.12) lower than estimated LTI.ConclusionSCI is a simple, easily obtainable and clinically relevant surrogate marker of MM in HD patients.
A systematic literature review of efficacy, effectiveness and safety of biologic therapies for treatment of familial Mediterranean fever
Authors: Kuemmerle-Deschner Jasmin B , Gautam Raju , George Aneesh T , Raza Syed , Lomax Kathleen G , Hur Peter 
Published: April 2020

Abstract

Objectives
To identify and summarize the existing evidence on the efficacy, effectiveness and safety of biologic therapies used, either as indicated or off-label, in the treatment of FMF.

Methods
A systematic literature review was conducted using Embase®, MEDLINE®, MEDLINE®-In Process, and Cochrane databases to identify randomized/non-randomized controlled trials (RCTs/non-RCTs) and real-world observational studies of FMF published as full-text articles (2000–September 2017) or conference abstracts (2014–September 2017). Studies with data for ≥1 biologic were included. Studies with &lt;5 patients were excluded.

Results
Of the 3342 retrieved records, 67 publications, yielding 38 unique studies, were included. All studies were published after the year 2010, and the majority (21) were full-text articles. Most studies (33/38) were prospective/retrospective observational; three were double-blind, placebo-controlled RCTs (one each of anakinra, canakinumab and rilonacept); and two were non-RCTs (both canakinumab). Anakinra (26), canakinumab (21) and etanercept (6) were the most frequently used biologics across studies, whereas use of adalimumab, tocilizumab, rilonacept and infliximab was limited (1–2 studies). The available evidence suggested benefits of anakinra and canakinumab in FMF.

Conclusion
Anti-IL-1 therapies (i.e. anakinra and canakinumab) appear to be effective and safe options in the treatment of overall FMF, including patients with colchicine resistance and FMF-related amyloidosis. There is a need for properly designed prospective or controlled studies to conclude the superiority of one anti-IL-1 therapy over another. Evidence on the use of TNF-α and IL-6 inhibitors is limited, and further research is suggested.