bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profiling of chromatin accessibility across Aspergillus species and identification of transcription factor binding sites in the Aspergillus genome using filamentous fungi ATAC-seq
Authors: Huang Lianggang , Li Xuejie , Dong Liangbo , Wang Bin , Pan Li 
Published: November 2019

AbstractTo identify cis-regulatory elements (CREs) and motifs of TF binding is an important step in understanding the regulatory functions of TF binding and gene expression. The lack of experimentally determined and computationally inferred data means that the genome-wide CREs and TF binding sites (TFBs) in filamentous fungi remain unknown. ATAC-seq is a technique that provides a high-resolution measurement of chromatin accessibility to Tn5 transposase integration. In filamentous fungi, the existence of cell walls and the difficulty in purifying nuclei have prevented the routine application of this technique. Herein, we modified the ATAC-seq protocol in filamentous fungi to identify and map open chromatin and TF-binding sites on a genome-scale. We applied the assay for ATAC-seq among different Aspergillus species, during different culture conditions, and among TF-deficient strains to delineate open chromatin regions and TFBs across each genome. The syntenic orthologues regions and differential changes regions of chromatin accessibility were responsible for functional conservative regulatory elements and differential gene expression in the Aspergillus genome respectively. Importantly, 17 and 15 novel transcription factor binding motifs that were enriched in the genomic footprints identified from ATAC-seq data of A. niger, were verified in vivo by our artificial synthetic minimal promoter system, respectively. Furthermore, we first confirmed the strand-specific patterns of Tn5 transposase around the binding sites of known TFs by comparing ATAC-seq data of TF-deficient strains with the data from a wild-type strain.
Bringing terroir back to the roots? A methodological proposal for studying local food products
Authors: Garçon Lucile 
Published: November 2019

Purpose
In line with various scientific papers warning against an inconsistent use of this adjective for food qualification, the purpose of this paper is to point out the sweeping assertion that “local” equates to “ecological”.

Design/methodology/approach
Looking beyond the measurement of carbon emissions to assess impacts on the environment, this paper addresses ecological issues in terms of interactions with the environment. To this end, it enhances an under-the-skin approach that goes through “local” fruit and vegetables to look into seed management and plant breeding practices.

Findings
This method, tested with 2 vegetative species – apple and potato – on 12 case studies in Europe, allows to build a typology that discriminates between: producing food without reproducing plants, grafting trees and storing tubers for maintaining landraces, and sowing seeds to restart the breeding process from the early beginning, trying in this way to enhance the capacity of plants to better fit with their environment. The typology matches a gradient that describes various degrees of intensity of environment–society relationships, from disconnection to adaptation – conceived on the one hand as already stabilized and on the other hand as still evolving.

Research limitations/implications
This analytical framework sheds light on contradictions that many local food networks have to face while yearning for a recognition by a geographical indication.

Originality/value
The paper argues that vegetal material might be a fruitful research object for tracking the controversies that unfold along the construction of local food products. It discusses social constructivist approaches of terroir while advocating for a materialist approach.
Political CSR initiatives as levers of marginalisation: the disconnect between representatives and the so-called represented in the mining industry
Authors: Roussey Clara , Balas Nicolas , Palpacuer Florence 
Published: November 2019

Purpose
The transformative potential of CSR is a far-reaching question. It has been analysed through the lens of the inclusion of stakeholders concerned by social and environmental issues in political CSR fora such as multi-stakeholder initiatives or, on the contrary, their exclusion from these processes. This paper aims to highlight the transformation or status quo produced by political corporate social responsibility (PCSR) initiatives, the extent of transformation being a function of the degree of inclusiveness, or conversely of exclusion, of these initiatives. From a promise of inclusion to the inability of corporate-society fora to act on the actual levers of marginalisation, PCSR scholars have developed contrasted views on these initiatives.

Design/methodology/approach
This led us to elaborate a hypothesis that such initiatives intrinsically act as levers in the recurring marginalisation of directly affected stakeholders. Drawing on an empirical study of the CSR discourses of mining industry stakeholders – both corporations and civil society – involved in an informal multi-stakeholder initiative, this paper discusses the disconnect between its representatives and the needs of the directly affected stakeholders.

Findings
To explore this disconnect, the authors draw on the voices and causes framework developed by Boltanski et al. (1984), which provided us with a relational system involving victims, guilty parties, complainants and judges.

Originality/value
Accordingly, the authors highlight a set of three interrelated marginalisation mechanisms (i.e. the capture of the role of the judge by PCSR initiatives, the side-lining of victims’ needs by complainants, the intertwining of the guilty party and the judge), which empirically support the lack-of-inclusiveness hypothesis.
Entertain me, I’ll stay longer! The influence of types of entertainment on mall shoppers' emotions and behavior
Authors: Elmashhara Maher Georges , Soares Ana Maria 
Published: November 2019

PurposeThe purpose of this paper is to provide a broader typology and inventory of recreational facilities and activities in shopping malls. The paper then investigates the relation between the proposed entertainment types and shopper behavior both directly and indirectly by considering the mediation of shopper emotional states.Design/methodology/approachA survey-based study with a sample size of 484 mall shoppers is used to test the proposed model.FindingsThe results indicate that permanent entertainment predicts shopper satisfaction directly and indirectly when considering the emotional state of pleasure as a mediator. Moreover, findings indicate that permanent entertainment indirectly influences the desire to stay at the mall through pleasure and arousal. In addition, pleasure mediates the relation of temporary and special event entertainment with satisfaction and the desire to stay at the mall.Practical implicationsThis study adds to a better understanding of the role of entertainment types in shopping malls. The study also reveals how each type of entertainment can enhance different emotions and behaviors. Practical suggestions to this end are offered.Originality/valueThere is a lack of studies taking into consideration the different types of entertainment in shopping malls. This study is among the first to examine the effects of each entertainment type on specific behavioral responses. Moreover, the study addresses the mediating role played by shoppers’ emotional states on the impact of entertainment types in satisfaction and desire to stay at the mall.
What drives green experiential loyalty towards green restaurants?
Authors: Wu Hung-Che , Cheng Ching-Chan , Ai Chi-Han 
Published: November 2019

Purpose
The purpose of this paper is to use a multi-level model as a framework to examine the relationships among experiential quality, green emotional attachment, green image, green switching experience, green experiential satisfaction and green experiential loyalty for the green restaurant industry.

Design/methodology/approach
The data used in this study were based on a sample of 515 customers at a green restaurant in Taipei City of Taiwan. Data were analyzed using structural equation modeling and hierarchical regression analysis.

Findings
The findings identify that interaction quality, peer-to-peer quality, physical environment quality, outcome quality, enjoyment quality and management quality influence experiential quality, which in turn results in green emotional attachment and green image respectively. Experiential quality, green image and green switching experience influence green experiential satisfaction, which in turn results in green emotional attachment. Green loyalty is influenced by green emotional attachment, green image, green experiential satisfaction and green switching experience. Green image moderates the relationships between experiential quality, physical environment quality and outcome quality.

Practical implications
To increase experiential quality and green emotional attachment, enhance green image, green experiential satisfaction and green loyalty and decrease green switching experience, the study findings will help green restaurant management develop and implement market-orientated service strategies.

Originality/value
This is the first study to investigate the effects of experiential quality, green emotional attachment, green image, green switching experience and green experiential satisfaction on green experiential loyalty in a green restaurant setting.
Performance management: a qualitative study of relational boundaries in personal assistance
Authors: Porter Tom , Shakespeare Tom , Stöckl Andrea 
Published: November 2019

AbstractPersonal assistance (PA) is a model of support where disabled people take control of recruiting, training and managing the people that support them. Personal assistance differs from other forms of care, such as domiciliary or informal care, because the disabled person is in control of how, when and by whom they are supported. With the advent of personal health budgets, PA is no longer limited to social care but is also central to future NHS services and funding arrangements. The aims of this study were to gain a deeper understanding of PA relationships, and to explore how both parties manage interpersonal challenges. We report on data from 58 qualitative interviews with disabled employers and personal assistants. Applying concepts from Goffman's (1959) scheme of impression management, we present an analysis of the relational dynamics that occur when two people cooperate in shared endeavours. Goffman's concepts of team members and non‐persons, in addition to the themes of regions and information control, aid a more fundamental understanding of the relational dynamics that occur between disabled employers and their PAs.
Taxonomic status of Chinese blue sheep (Pseudois nayaur): new evidence of a distinct subspecies
Authors: GAO Hui , LI Nannan , HUANG Yongjie , QIAO Fujie , LI Junle , LI Zongzhi , LI Yanxiang , WANG Zhenghuan , TENG Liwei , LIU Zhensheng 
Published: November 2019

AbstractThe blue sheep is an endemic species to the Qinghai‐Tibet Plateau and surrounding regions. It has been regarded as having 2 subspecies: Pseudois nayaur nayaur and P. n. szechuanensis. However, such a classification remains controversial. Herein, we analyze 10 microsatellite loci and part of the mitochondrial control region for clarification in such taxonomic debates. We use samples from 168 individuals from 6 geographic populations covering almost all the distribution areas of the species in China to carry out comparisons. Phylogenetic trees derived from both the microsatellite and mitochondrial markers combined with the discriminant analysis of principal components (DAPC) and the STRUCTURE analysis reveal that the individuals in the Helan Mountains are well grouped with a distinct evolutionary lineage and are significantly different from the other populations of P. n. szechuanensis according to Fst values, implying that this isolated population should be categorized as a valid subspecies; namely, Pseudois nayaur alashanicus. The isolation‐by‐distance (IBD) analysis shows a significant positive relationship between genetic and geographical distances among the populations.
Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression
Authors: Dang Yao , An Yongpan , He Jinzhao , Huang Boyue , Zhu Jie , Gao Miaomiao , Zhang Shun , Wang Xin , Yang Baoxue , Xie Zhengwei 
Published: November 2019

AbstractAlthough aging and senescence have been extensively studied in the past few decades, however, there is lack of clinical treatment available for anti‐aging. This study presents the effects of berberine (BBR) on the aging process resulting in a promising extension of lifespan in model organisms. BBR extended the replicative lifespan, improved the morphology, and boosted rejuvenation markers of replicative senescence in human fetal lung diploid fibroblasts (2BS and WI38). BBR also rescued senescent cells with late population doubling (PD). Furthermore, the senescence‐associated β‐galactosidase (SA‐β‐gal)‐positive cell rates of late PD cells grown in the BBR‐containing medium were ~72% lower than those of control cells, and its morphology resembled that of young cells. Mechanistically, BBR improved cell growth and proliferation by promoting entry of cell cycles from the G0 or G1 phase to S/G2‐M phase. Most importantly, BBR extended the lifespan of chemotherapy‐treated mice and naturally aged mice by ~52% and ~16.49%, respectively. The residual lifespan of the naturally aged mice was extended by 80%, from 85.5 days to 154 days. The oral administration of BBR in mice resulted in significantly improved health span, fur density, and behavioral activity. Therefore, BBR may be an ideal candidate for the development of an anti‐aging medicine.
When to consider electroconvulsive therapy (ECT)
Authors: Kellner C. H. , Obbels J. , Sienaert P. 
Published: November 2019

ObjectiveTo familiarize the reader with the role of electroconvulsive therapy (ECT) in current psychiatric medicine.MethodWe review clinical indications for ECT, patient selection, contemporary ECT practice, maintenance treatment and ECT in major treatment guidelines.ResultsECT is underutilized largely due to persisting stigma and lack of knowledge about modern ECT technique.ConclusionECT remains a vital treatment for patients with severe mood disorders, psychotic illness and catatonia.
The complex TIE between macrophages and angiogenesis
Authors: Du Cheyne Charis , Tay Hanna , De Spiegelaere Ward 
Published: November 2019

AbstractMacrophages are primarily known as phagocytic immune cells, but they also play a role in diverse processes, such as morphogenesis, homeostasis and regeneration. In this review, we discuss the influence of macrophages on angiogenesis, the process of new blood vessel formation from the pre‐existing vasculature. Macrophages play crucial roles at each step of the angiogenic cascade, starting from new blood vessel sprouting to the remodelling of the vascular plexus and vessel maturation. Macrophages form promising targets for both pro‐ and anti‐angiogenic treatments. However, to target macrophages, we will first need to understand the mechanisms that control the functional plasticity of macrophages during each of the steps of the angiogenic cascade. Here, we review recent insights in this topic. Special attention will be given to the TIE2‐expressing macrophage (TEM), which is a subtype of highly angiogenic macrophages that is able to influence angiogenesis via the angiopoietin–TIE pathway.
Endotoxin‐induced changes in expression of cyclooxygenase isoforms in the lamellar tissue of extracorporeally haemoperfused equine limbs
Authors: Patan‐Zugaj Bianca , Egerbacher Monika , Licka Theresia F. 
Published: November 2019

AbstractAngiogenesis and sepsis‐related equine laminitis have several features in common. Both events can be induced by endotoxin (lipopolysaccharide— LPS) and both are associated with increased expression of the enzyme cyclooxygenase (COX), of which two isoforms (COX‐1 and COX‐2) exist. To examine the causal relationship between LPS exposure and COX expression and to investigate the tissue distribution of COX in the LPS‐exposed tissue, the technique of extracorporeal haemoperfusion of isolated equine forelimbs was utilized. Perfusion was performed for 10 hr under physiological conditions (control‐perfused limbs,n = 5) and with addition of 80 ng/L of endotoxin (LPS‐perfused limbs;n = 5). After perfusion, samples of lamellar tissue were collected from the dorsal aspect of the hoof wall. Additional control samples were collected from three non‐perfused limbs. Immunohistochemical analysis was performed using antibodies against COX‐1 and COX‐2, and intensity of immunohistochemical staining was scored for each isoform. In the lamellar tissue of control‐ and LPS‐perfused limbs, there was no significant difference in COX‐1 staining intensity and distribution, whereas COX‐2 expression was significantly increased in LPS‐perfused limbs (especially in endothelial cells, fibroblasts and intravasal leucocytes as well as in epidermal basal cells at the base of the primary epidermal lamellae). These results suggest that COX‐2 and its metabolites are involved in the initiation of pathological changes seen in sepsis‐associated events such as sepsis‐related laminitis. In such cases, COX‐2 could therefore be an important therapeutic target; however, early therapy may be required as increase in COX‐2 expression occurs within 10 hr after LPS exposure.
Systematic use of the serum C‐reactive protein concentration and computed tomography for the detection of intestinal anastomotic leaks
Authors: Ho Yiu Ming , Laycock Juliet , Kirubakaran Arangan , Hussain Labib , Clark Jeremy 
Published: November 2019

AbstractBackgroundThis study aimed to characterize the time‐dependent relationship between serum C‐reactive protein (CRP) and anastomotic integrity in the early post‐operative period and to develop a systematic use of CRP and computed tomography.MethodsPatients aged 18 years or over who had the formation of a left‐sided colonic or a colorectal anastomosis, in Royal Sussex County Hospital, were included. The post‐operative day (POD) CRP cut‐off values were calculated according to receiver operating characteristic analysis to evaluate the sensitivities and specificities of the proposed cut‐off parameters.ResultsA total of 125 left‐sided colonic and colorectal anastomoses were recruited and analysed. When comparing to POD1 CRP cut‐off, the calculated CRP ratio cut‐off values of all the rest of PODs (2–5) were highly significant in the laparoscopic group and the overall group (P < 0.001). This statistically significant ratio was also demonstrated in the open group at POD2 (P < 0.0001).ConclusionCRP and CRP ratios cut‐off values were sensitive to detect an anastomotic leak in the early post‐operative period. The cut‐off values could facilitate the development of systematic use of CRP and computed tomography.
Non‐obstetric abdominal surgery during pregnancy and birth outcomes: A Danish registry‐based cohort study
Authors: Rasmussen Anne S. , Christiansen Christian F. , Ulrichsen Sinna P. , Uldbjerg Niels , Nørgaard Mette 
Published: November 2019

AbstractIntroductionSurgery during pregnancy may increase the risk of adverse birth outcomes. In this nationwide registry‐based cohort study including women aged 15‐54 years with singleton birth or miscarriage, we examined the association between non‐obstetric abdominal surgery during pregnancy and the birth outcomes small‐for‐gestational‐age (SGA), preterm birth, and miscarriage.Material and methodsThe study used data on births or miscarriages from the large national Danish registries in 1997‐2015. We calculated absolute risks and risk differences for the main outcomes and used Cox regression analysis with non‐obstetric abdominal surgery as a time‐varying exposure, adjusting for maternal age, year of last menstrual period, major abdominal surgery before pregnancy, maternal smoking status, rheumatoid arthritis, diabetes and inflammatory bowel disease. Our main outcome measures were risks and hazard ratios (HRs) for SGA, very preterm or preterm birth, and miscarriage after gestational week 7 overall, stratified by calendar year, and, for SGA, trimester of pregnancy. Finally, absolute risk of miscarriage stratified by time since surgery.ResultsAbsolute risks in surgically treated vs untreated were 3.4% vs 2.7% for SGA (adjusted HR 1.3, 95% CI 1.1‐1.5), 2.2% vs 0.8% for very preterm birth (adjusted HR 2.8, 95% CI 2.2‐3.5), 8.3% vs 4.3% for preterm birth (adjusted HR 2.1, 95% CI 1.9‐2.3), and 8.2% vs 6.1% for miscarriage (adjusted HR 3.1, 95% CI 2.7‐3.5). For miscarriage, the risk was highest the first week after surgery and levelled out after 2 weeks.ConclusionsSurgery during pregnancy is associated with an increased risk of SGA, very preterm birth, preterm birth and miscarriage, and the risk of miscarriage is highest the first week after surgery.
Ciliary muscle dimensions by swept‐source optical coherence tomography and correlation study in a large population
Authors: Fernández‐Vigo José Ignacio , Shi Hang , Kudsieh Bachar , Arriola‐Villalobos Pedro , De‐Pablo Gómez‐de‐Liaño Lucía , García‐Feijóo Julián , Fernández‐Vigo José Ángel 
Published: November 2019

AbstractPurposeTo examine ciliary muscle (CM) dimensions in vivo by swept‐source optical coherence tomography (SS‐OCT) in a large healthy population. To assess the reproducibility of CM measurements and correlations with different parameters.MethodsIn this cross‐sectional study, the following CM measurements were made in 495 eyes of 495 subjects in the temporal and nasal quadrants: length (CML), area (CMA) and thickness at distances of 1000 μm (CMT1), 2000 μm (CMT2) and 3000 μm (CMT3) from the scleral spur using the SS‐OCT DRI‐Triton® (Topcon, Tokyo, Japan). These dimensions were then assessed for correlations with the factors age, gender, refractive error, anterior chamber angle (ACA) and intraocular pressure (IOP). The reproducibility of CM measurements was determined in 85 of the participants.ResultsMean age was 41.4 ± 17.5 years (range 5–83). The following means were recorded as follows: CML = 4.57 ± 0.73 mm (range 2.16–6.97), CMA = 1.35 ± 0.31 mm2 (2.04–5.45), CMT1 = 558 ± 98 μm (299–891), CMT2 = 366 ± 100 μm (89–629) and CMT3 = 210 ± 75 μm (36–655). Negative correlation was detected between CML/CMA and age (R = −0.516/R = −0.404; p < 0.001) and refractive error (R = −0.519/R = −0.538; p < 0.001). No gender differences were observed (p ≥ 0.150). Both CML and CMA were correlated with ACA (R = 0.498 and 0.546; p < 0.001) and slightly with IOP (R = −0.175; p < 0.001). The reproducibility of the CM measurements was excellent (intraclass correlation coefficient ≥0.878).ConclusionsSwept‐source optical coherence tomography is a valid tool for in vivo CM measurements. Our data indicate a larger CM in myopes and its diminishing size with age.