bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a Prognostic Model for Predicting Overall Survival of Patients with Colorectal Cancer
Authors: Han Shuwen , Ding Kefeng 
Published: May 2020

Abstract
Background: Colorectal cancer (CRC) is one of the most common malignancies. The purpose of this study is to construct a prognostic model for predicting the overall survival (OS) in patients with CRC. Methods: The mRNA-seq and miRNA-seq data of colon adenocarcinoma (COAD) and rectal adenocarcinoma (READ) were downloaded from The Cancer Genome Atlas (TCGA) database. The differentially expressed RNAs (DE-RNAs) between tumor and normal tissues were screened. The Kaplan-Meier and univariate Cox regression analysis were used to screen the survival-related genes. Functional enrichment analysis of survival-related genes was conducted, followed by protein-protein interaction (PPI) analysis. Subsequently, the potential drugs targeting differentially expressed mRNAs (DE-mRNAs) were investigated. Multivariate Cox regression analysis was then conducted to screen the independent prognostic factors, and these genes were used to establish a prognostic model. A receiver operator characteristic (ROC) curve was constructed, and the area under the curve (AUC) value of ROC was calculated to evaluate the specificity and sensitivity of the model. Results: A total of 855 survival-related genes were screened. These genes were mainly enriched in Gene Ontology (GO) terms, such as methylation, synapse organization, and methyltransferase activity; and pathway analysis showed that these genes were significantly involved in N-Glycan biosynthesis and the calcium signaling pathway. PPI analysis showed that aminolevulinate dehydratase (ALAD) and cholinergic receptor muscarinic 2 (CHRM2) served vital roles in the development of CRC. Aminolevulinic acid, levulinic acid, and loxapine might be potential drugs for CRC treatment. The prognostic models were built and the patients were divided into high-risk and low-risk groups based on the median of risk score (RS) as screening threshold. The OS for patients in the high-risk group was markedly shorter than that for patients in the low-risk group. Meanwhile, kazal type serine peptidase inhibitor domain 1 (KAZALD1), hippocalcin like 4 (HPCAL4), cadherin 8 (CDH8), synaptopodin 2 (SYNPO2), cyclin D3 (CCND3), and hsa_mir_26b may be independent prognostic factors that could be considered as therapeutic targets for CRC.Conclusion: We established prognostic models that could predict the OS for CRC patients and may assist clinicians in providing personalized and precision treatment in this patient population.Highlights:1. ALAD served a vital role in the development of CRC.2. CHRM2 played a role in CRC development by affecting the calcium signaling pathway.3. Aminolevulinic acid, levulinic acid, and loxapine might be potential drugs for treating CRC.4. KAZALD1 and HPCAL4 were associated with the OS of CRC.5. CDH8, SYNPO2, CCND3, and hsa-mir-26b were closely related to the prognostic of CRC staging.
Cryopreservating Human Dental Pulp Tissue at Tooth Extraction for Later Culturing Proliferative and Multipotent Cells
Authors: Yan Ming , Fu Lingling , Kluwe Lan , Li Xiangjun , Ren Guiyun , Smeets Ralf , Gosau Martin , liu xiao 
Published: May 2020

Abstract
Background Teeth extracted are usually disposed as bio-waste whereas they could serve as an autologous tissue for culturing multipotent dental pulp cells which have application potential in regenerative medicine. The present study aims to examine the feasibility of cryopreservating dental pulp tissue at teeth extraction for later culturing cells. Methods The pulp tissue from each of a total of 10 teeth were cut into small fragments which were then divided into two portions. One portion was directly used for culturing pulp cells using the explant method. The other portion was cryopreserved with 10% DMSO in liquid nitrogen for at least one month and then thawed for culturing pulp cells. Results Vital cells were obtained from all the 10 pulp fragment suspensions which went through cryopreservation. The cell outgrowth from the explants of cryopreserved pulp fragments was two days later than that of corresponding fresh pulp tissue. Otherwise, no difference was observed in proliferation, expression of stem cell markers and differentiation into adipose cells and osteoblasts between the two groups of cells cultured from the fresh or the cryopreserved pulp fragments. Conclusions Cryopreservating fragmented dental pulp tissue therefore provides a feasible option for saving pulp tissues as autologous cells sources for possible later application.
Dental caries experience in adults: a cross-sectional community survey within Ethiopia
Authors: Bogale Birke , Engida Fasikawit , Hanlon Charlotte , Prince Martin J , Gallagher Jennifer E 
Published: May 2020

Abstract
Background Ethiopia is a large and diverse developing sub-Saharan African country with increasing prevalence of non-communicable diseases, including oral conditions. Oral health and dental care have been given little consideration, and there is limited information relating to population oral health and use of dental services in the country. The aim of this study was to examine the burden and impact of dental caries experience and investigate access to dental care amongst adults within Ethiopia. Methodology This community-based oral health survey is a baseline study for the ASSET - Health System Strengthening in sub-Saharan Africa project undertaken in the Butajira area, south-central Ethiopia. A stratified random sample of households and individuals were participated in the study. The survey was informed by the World Health Organization (WHO) Oral Health Survey Methods (5th ed.) at International Caries Detection and Assessment System (ICDAS) threshold 4 and the UK Adult Dental Health Survey 2009. Face-to-face interviews and clinical dental examinations were conducted. The data were analysed using descriptive statistics; and a binary logistic regression model was built to assess the association of dental caries and predictor variables: demographics, health behaviours, quality of life, dentition status and consequences of untreated caries data in adults (≥18 years).Result Most of the study population (n=626) were female (63.9%), married (71.4%) and Muslim (76.0%). Just over half (53.2%) lived in rural areas and many (44.4%) had no formal education. A majority (74.0%) reported never attending a dental service. Sixty percent (n=377) of the adults had experienced dental caries, 88.0% (n=332) of whom had untreated carious teeth. In the fully adjusted logistic regression model; increasing age, dental attendance and Khat chewing had a positive significant association with dental caries experience, whilst education status was negatively associated (p<0.05).Conclusion This study demonstrated a high burden of dental caries and considerable impact resulting from untreated disease in this population of adults. There was also evidence of social inequity and limited overall access to dental care, highlighting the need for health system strengthening.
SENP1 Knockdown Suppresses Tumor Progression in Lung Adenocarcinoma by Regulating AAR Genes Expression
Authors: Cheng Hong , Li Renda , Wang Feng , Yu Xiangyang , Wang Feng , Gao Yi-Bo , Mu Juwei , He Jie 
Published: May 2020

Abstract
Background SUMO specific peptidase 1(SENP1) is an important factor involved in the regulation of small ubiquitin-related modifier (SUMO) modification. Our previous research has shown that SENP1 could be a potential tumor-promoting factor in non-small cell lung cancer (NSCLC). However, its role in tumor progression remains largely unknown. This study aims to characterize the role of SENP1 in lung adenocarcinoma. Methods The TCGA database provided us expression profiles of SENP1 and overall survival rates. loss-of-function assays were performed to examine the effect of SENP1 on proliferation, migration and invasion of lung adenocarcinoma cells in vitro and in vivo. Immunoprecipitation (IP), western blot and quantitative real time PCR (qRT-PCR) were carried out to reveal the interrelation between SENP1, SIRT6 and AAR (amino acid response) genes signal pathway. Results In this study, we found that SENP1 was expressed at high levels in lung adenocarcinoma tissues and advanced TNM stages and was significantly associated with poor prognosis. we also found that SENP1 knockdown inhibited lung adenocarcinoma cell progression in vitro and in vivo. SUMOylation of SIRT6 was also observed in lung adenocarcinoma, and it was reduced by SENP1. SUMOylation of SIRT6 specifically increased its deacetylation of histone H3 on lysine 56 instead of that of lysine 9 (H3K9) in an in vitro model. Mechanistically, we found that knockdown of SENP1 reduced the expression of AAR genes by decreasing H3K56 acetylation through increasing SIRT6 SUMOylation. Moreover, mutation of the SUMOylation sites of Sirt6 reduced its tumor-suppressive effects. Conclusions These results revealed that SENP1 promotion of tumor progression in lung adenocarcinoma and its tumor-promoting effects might be attributed to its important role in the regulation of Sirt6 SUMOylation and the expression of AAR genes.
Downregulation of the lncRNA RP11-432I5.4 inhibits tumorigenesis in the JAK2V617F-positive classic myeloproliferative neoplasms
Authors: Zhou Jie , Wu Hao , Zhang Wen-Jun , Fu Jian-Fei , Liang Ai-Bin 
Published: May 2020

Abstract
Background: Although aberrant expression of long non-coding RNA (lncRNA) is associated with many human cancers, little is known about its role in the JAK2V617F-positive classic myeloproliferative neoplasms (cMPNs). Methods: In this study, we performed a comprehensive analysis of lncRNAs in human cMPNs cells using a lncRNA cDNA microarray and identified the lncRNA RP11-432I5.4 acted as an effective factor in JAK2V617F-positive cMPN cells. Results: The lncRNA RP11-432I5.4 showed higher expression in cMPN patients, especially higher in JAK2V617F-positive cells compared with JAK2V617F-negative cells. Overexpression of lncRNA RP11-432I5.4 increased the proliferation of JAK2V617F-positive cells while downregulation of it decreased proliferation, promoted apoptosis and triggered S phase arrest of JAK2V617F-positive cells. Furthermore, in a mouse xenograft model, the silencing of lncRNARP11-432I5.4 repressed tumor formation in vivo. Conclusions: Taken together, these results revealed that lncRNA RP11-432I5.4 plays an important role in cMPN tumorigenesis and may be a potential novel target for treatment of JAK2V617F-positive cMPN patients.
Effect of diversified meals only, or either enriched with Amaranthus tricolor and Moringa oleifera leaf powder or commercial micronutrient powder on prevention/treatment of anemia in Adivasi children, West Bengal, India
Authors: Stiller Caroline Katharina , Golembiewski Silvia Konstanze Ellen , Stuetz Wolfgang , Golembiewski Monika , Rincon Daniela Gomez , Mondal Srikanta , Biesalski Hans Konrad , Scherbaum Veronika 
Published: May 2020

Abstract
Background and objectives: Malnutrition associated with anemia remains a leading cause of morbidity and mortality among Adivasi children in India. The present trial aimed to test three possible ways of designing improved supplementary meals and to define their role in decreasing rates of anemia, thus increasing hemoglobin (Hb) concentrations as primary health objective: diversified meals only (intervention group 1 (IG 1)), with the addition of locally producible Amaranthus tricolor/Moringa oleifera leaf powders (ALP/MLP) in the ratio 2:1 (IG2) or with an adjusted amount of commercially produced micronutrient powder TopNutri (IG3). Methods: Cluster-randomization of 21 villages resulted in the inclusion of n=293 children aged 6-39 months after baseline assessment. The trial duration was 18 months, beginning in February 2015 (baseline assessment), with application of study meals three times a week at community level. Anthropometric, Hb (HemoCue201+) and morbidity data were collected in a total of four assessment points. At baseline a socio-economic questionnaire was performed. Results: Adjusted for age and Hb concentrations at baseline, time between assessment points, and gender; IG1 showed significant higher Hb concentrations as compared to the control group (CG) throughout the intervention period. The Hb of the remaining intervention groups IG2 or IG3, remained comparable to the CG at all assessment points. The effect on growth indices was less consistent, however most positive tendencies related to nutrition status (stunting, underweight, wasting) and morbidity reduction were attributable to IG3, indicating the beneficial role of a holistic nutrient composition in addressing undernutrition and infectious diseases. Conclusion: This low-dose intervention trial proofed the feeding of diversified diets alone (IG1) to be sufficient to significantly increase Hb concentrations of study children. On the way of achieving diversified diets for Santal children the promotion of kitchen garden programs combined with interactive awareness trainings may be a key measure. Trial registration: the trial was retrospectively registered at the German Clinical Trials Register on the 1 st July 2019 (DRKS00017388). URL: https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00017388
A study on COVID-19 transmission dynamics: Stability analysis of SEIR model with Hopf bifurcation for effect of time delay
Authors: M.Rasha  , S.Balamuralitharan  
Published: May 2020

Abstract
This paper deals with a general SEIR model for the coronavirus disease 2019 (COVID-19) with the effect of time delay proposed. We get the stability theorems for disease-free equilibrium and provide adequate situations of the COVID-19 transmission dynamics equilibrium of present and absent cases. A Hopf bifurcation parameter $\tau$ is the effects of time delay and we demonstrate that the locally asymptotic stable is present equilibrium. The Reproduction number is brief in less than or greater than one, and it effectively controlling the COVID-19 infection outbreak, and subsequently reveals insight into understanding the patterns of the flare-up. The numerical experiment is calculated to help the theoretical outcomes.
A REPRESENTATIVIDADE DA BANCADA EVANGÉLICA NO ESTADO LAICO: Análise de projetos apresentados ao Congresso Nacional.
Authors: ALMEIDA Bruno Lopes de 
Published: May 2020

O presente projeto de pesquisa pode ser justificado, vez que, a Constituição Federal de 1988 preconizou um Estado laico paralelo ao desrespeito dos mesmos princípios e garantias fundamentais nela contidos. Significa conferir historicamente e legalmente as ações tomadas pela bancada evangélica e tem como norteador o texto constitucional e a Declaração Universal dos Direitos Humanos (DUDH). Todo esse processo justifica a necessidade crescente de análises profunda na sociedade e como refletem as políticas públicas nas parcelas excluídas (ou com menor representatividade) como religiões de origem africanas, a sociedade LGBTQI+ e o Conservadorismo imposto.
La dietética como una elección de vida: una mirada desde la medicina hipocrática
Authors: Maca Sanchez Marlyn Patricia 
Published: May 2020

El Corpus Hippocraticum, corresponde a la primera colección de escritos médicos griegos; donde se halla la medicina hipocrática. Esta colección se colocó sin excepción bajo el nombre de Hipócrates de Cos, al reunirse en la Biblioteca de Alejandría a mediados o finales del siglo III a.C. Hallamos en él, que el médico hipocrático para el tratamiento de la enfermedad así como para la conservación de la salud formula una dieta, que está conformada por un conjunto de prácticas diarias. En el presente estudio se analizan algunas obras del Corpus, que versan sobre dietética, y se intenta evidenciar que la dieta (díaita) en la antigüedad al estar constituida por alimentos (sîta), bebidas (potá) y ejercicios (pónoi), prácticas que recorren la vida diaria del hombre; expresan una elección de vida, que surge de una conciencia sobre una salud que se debe cuidar, y es sinónimo de equilibrio; es lo que conviene al hombre.
Application of unsupervised learning and process simulation for energy optimization of a WWTP under various weather conditions
Authors: Borzooei Sina , Miranda Gisele H. B. , Abolfathi Soroush , Scibilia Gerardo , Meucci Lorenza , Zanetti Maria Chiara 
Published: May 2020

Abstract
This paper outlines a hybrid modeling approach to facilitate weather-based operation and energy optimization for the largest Italian wastewater treatment plant (WWTP). Two clustering methods, K-means algorithm and Gaussian mixture model (GMM) based on the expectation-maximization (EM) algorithm, were applied to an extensive dataset of historical and meteorological records. This study addresses the problem of determining the intrinsic structure of clustered data when no information other than the observed values is available. Two quantitative indexes, namely the Bayesian information criterion (BIC) and the Silhouette coefficient using Euclidean distance, as well as two general criteria, were implemented to assess the clustering quality. Furthermore, seven weather-based influent scenarios were introduced to the process simulation model, and sets of aeration strategies are proposed. The results indicate that incorporating weather-based aeration strategies in the operation of the WWTP improves plant energy efficiency.
Prompt nitrogen removal by controlling the oxygen concentration in sediment microbial fuel cell systems: the electrons allocation and its microbial mechanism
Authors: Sima Weiping , Ma Ruixiang , Yin Feixian , Zou Haodong , Li Hong , Ai Hainan , Ai Tao 
Published: May 2020

AbstractIt has been proved that the nitrogen can be removed from the sediment in a sediment microbial fuel cell system (SMFCs), but the competition between nitrate and oxygen for electrons would be a key factor that would affect the removal efficiency, and its mechanism is not clear. Based on organic sediment fuel, an SMFC was constructed, and the influence of dissolved oxygen (DO) on nitrogen transformation and cathodic microbial communities was investigated. The results showed that the best total nitrogen removal efficiency of 60.55% was achieved at DO level of 3 mg/L. High DO concentration affected the removal efficiency through the electrons' competition with nitrate, while low DO concentration suppressed the nitrification. Comamonas, Diaphorobacter and Brevundimonas were the three dominant genera responsible for denitrification at DO concentration of 3 mg/L in this study. The establishment of SMFCs for nitrogen removal by regulating DO level would offer a promising method for sediment treatment.
DISSOLUTION ENHANCEMENT AND FORMULATION OF FILM COATED TABLETS OF LORNOXICAM BY PHASE TRANSITION METHOD: IN VITRO AND IN VIVO EVALUATION
Authors: ABDELMONEM REHAB AHMED , GALIL RANIA MOSTAFA ABD EL , EL-SETOUHY DOAA AHMED , EL-MILIGI MOHAMED FARID , EL-NABARAWI MOHAMED AHMED 
Published: May 2020

Objective: This study aimed to enhance the oral solubility and dissolution of poorly soluble lornoxicam by anti-solvent precipitation, and the manufacture of oral tablets by the phase transition method.
Methods: The solvent was mixture of polyethylene glycol 400 and absolute ethanol. Three stabilizers Inutec SP1, Pluronic F127, Sucrose ester S1670 at two concentrations and two matrix formers Mannitol, and Avicel PH102 were used to obtain 12 formulae. The formulae were characterized regarding their infrared spectroscopy (IR), differential scanning calorimetry (DSC), particle size (PS) measurement, drug content and dissolution. Further characterizations were done for the optimum formula by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Four tablet formulae were manufactured by phase transition method. The optimum tablets (T3) were evaluated through hardness, drug content, disintegration, dissolution, IR, and stability studies. Finally, (T3) was compared to conventional tablets in New Zealand rabbits using crossover design.
Results: The dissolution rate for the prepared formulae was enhanced, from 3.44 to 5.96 folds. Statistical significance was obtained using one and two way ANOVA among formulae. The optimum tablet formula (T3) had hardness 5.637±1.57 kg, drug content 90.424±1.19%, disintegration time 341.5±9.62 s and the drug dissolved 72.107±0.0025%. Stability, after one month storage of the selected tablets at (25 °c/60% relative humidity), was satisfactory. The absorption extent of lornoxicam from (T3) compared to the conventional tablets was higher.
Conclusion: Taken together, the obtained results confirmed successfully the potential of the promising formula (T3), over the conventional tablets of lornoxicam.
The applicability of bio-carrier BioChip in Sequencing Batch Reactors
Authors: Tran Ha Quan  , Gogina Elena S. 
Published: May 2020

Introduction. All over the world, the microbial biofilm has been regularly applied to modify urban and industrial wastewater treatment facilities that have aeration tanks. Unlike the activated sludge technology, the biofilm contains a substantial number of bacteria because microorganisms get attached to the surface of the material and grow there. The co-authors share their opinion about the BioChip, applied inside SBR, a sequencing batch reactor. This technology contemplates the use of free-floating activated sludge when all water treatment phases are concentrated inside one tank.
Materials and methods. To assess the usability of the BioChip inside the SBR reactor, experiments were conducted in an attempt to remove two types of contaminants: organic substances inside the standard model of an SBR reactor, and biogenic elements, in particular, ammonium nitrogen, in the course of developing an anoxic zone in a modified model of the SBR reactor. The per-unit speed of substrate recycling, nitrification and denitrification is determined as a result of the analysis of the quality of wastewater and treated water.
Results.The experiment conducted using the laboratory model of the SBR reactor has proven the water treatment efficiency within the range of 80–90 % in terms of organic substances and 75–85 % in terms of ammonium nitrogen. Inside the SBR reactor, that had a BioChip installed, the per-unit denitrification speed inside the reactor reached 0.3 to 0.4 N/kg of sludge/day, for the process of nitrification, the value of speed varies from 0.18 to 0.2 kg N/kg of sludge/day. Per-unit speed values and the nitrogen balance equation inside the SBR reactor suggest the SBR operating mode at the capacity of 1.5–2.5 kg of the biological oxygen demand–TKN/kg of sludge/day.
Conclusions. Low costs, smooth transportation and use in combination with the pre-set SBR reactor with a view to intensification of water treatment efficiency are the main strengths of the BioChip. The operation of the SBR reactor in combination with the BioChip and nitrification/denitrification phases requires highly skilled personnel.