bluuebunny/crossref_metadata_2025_split huggingface dataset:
Necrotizing Funisitis As an Intrauterine Manifestation of Cryopyrin-Associated Periodic Syndrome: A Case Report and Review of The Literature
Authors: Yokoi Kyoko , Minamiguchi Sachiko , Honda Yoshitaka , Kobayashi Mizuho , Kobayashi Satoru , Nishikomori Ryuta 
Published: September 2020

Abstract
Background: Cryopyrin-associated periodic syndrome (CAPS) is a life-long, autoinflammatory disease associated with a gain-of-function mutation in the nucleotide-binding domain, leucine-rich repeat family, pyrin domain containing 3 (NLRP3) gene, which result in uncontrolled production of IL-1β and chronic inflammation. Chronic infantile neurologic cutaneous and articular (CINCA) syndrome/neonatal-Onset multisystem inflammatory disease (NOMID) is the most severe form of CAPS. Although the first symptoms may be presented at birth, there are few reports on the involvement of the placenta and umbilical cord in the disease. Therefore, we present herein a preterm case of CINCA/NOMID syndrome and confirms intrauterine-onset inflammation with conclusive evidence by using fetal and placental histopathological examination. Case presentation: The female patient was born at 33weeks of gestation by emergency caesarean section and weighted at 1,514 g. The most common manifestations of CINCA/NOMID syndrome including recurrent fever, urticarial rash, and ventriculomegaly due to aseptic meningitis were presented. She also exhibited atypical symptoms such as severe hepatosplenomegaly with cholestasis. The genetic analysis of NLRP3 revealed a heterozygous c.1698C>A (p.Phe566Leu) mutation, and she was diagnosed with CINCA/NOMID syndrome. Further, a histopathological examination revealed necrotizing funisitis, mainly inflammation of the umbilical artery, along with focal neutrophilic and lymphocytic villitis. Conclusion: The necrotizing funisitis, which only involved the artery, was an unusual observation for chorioamnionitis. These evidences suggest that foetal inflammation, probably due to overproduction of IL-1β, caused tissue damage in utero, and the first symptom of a newborn with CINCA/NOMID.
Vibration Preconditioning as a Method to Reduce Hyperemia Responses to Plantar Pressure-Induced Ischemia
Authors: Liu Hongmei , Ren Weiyan , Duan Yijie , Ye Wenqiang , Jan Yih-Kuen , Pu Fang , Fan Yubo 
Published: September 2020

Abstract
Background: One of the main causes for foot ulcers in diabetics is an excessive, constant load on the foot which over time leads to ischemia. The aim of this study is to explore whether vibration preconditioning can alleviate pressure-induced hyperemia responses in foot tissue.Methods: Fifteen healthy subjects were subjected to compression stimulation with or without vibration preconditioning, determined randomly. Skin blood flow and temperature were measured under the first metatarsal head of the right foot for each subject and compared across the test group.Results: The results showed that both test groups displayed a reactive hyperemia response, but the peak hyperemia was significantly decreased when vibration was used in combination with compression. In the group subjected to compression only (no vibration), the plantar skin temperature during the first minute after compression was significantly higher than the basal temperature, but this was not so when vibration was applied.Conclusions: The results of this study suggest that vibration preconditioning before the application of compression can decrease the degree of reactive hyperemia and alleviate pressure-induced ischemic damage. These findings may be used to develop methods to protect against pressure-induced foot lesions in diabetic people.
A Treatment Combined Prussian Blue Nanoparticles With Low-intensity Pulsed Ultrasound Alleviates Cartilage Damage in Knee Osteoarthritis by Initiating PI3K/Akt/mTOR Pathway
Authors: Zuo Deyu , Tan Botao , Jia Gongwei , Wu Dandong , Yu Lehua , Jia Lang 
Published: September 2020

Abstract
Background: Reactive oxidative stress (ROS) related apoptosis in chondrocytes and extracellular matrix (ECM) degradation play crucial roles in the process of osteoarthritis (OA). Prussian blue nanoparticles (PBNPs) are known to scavenge ROS in cellular. Low-intensity pulsed ultrasound (LIPUS) has been used as a non-invasive modality for the is widely used in clinical rehabilitation management of OA. Methods: In this study, we aim to investigate the effects of PBNPs/LIPUS combined treatment on knee osteoarthritis (KOA) and to determine whether phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway mediates this process. Use LPS to process primary cells of knee joint cartilage to establish a cartilage knee arthritis model. After treated with LIPUS and PBNPs, cell viability was rated by CCK-8 and ROS levels were assessed by DCFH-DA. Cell apoptosis was estimated by flow cytometry and TUNEL staining. Articular pathological changes were observed by naked eyes, H&E, and Safranin O staining, then monitored by cartilage lesion grades and Mankin’s score. Protein levels of cleaved caspase-3, Bcl-2, Bax, p-PI3K, PI3K, p-Akt, Akt, p-mTOR, mTOR, IL-1β, MMP3, MMP13, p-JINK, JINK, p-c-Jun, and c-Jun were subjected to western blot. Results: Cellular ROS, apoptosis rate, and TUNEL staining of chondrocytes were fairly decreased in the PBNPs group and the LIPUS group but drastically down-regulated in the PBNPs/LIPUS combination treatment group when compared with the LPS group. Both PBNPs and LIPUS decreased the grades of cartilage lesions and Mankin scores of knee articular cartilage, while combined administration achieved more reduction. Western blot results showed that the cleaved caspase-3, Bax, IL-1β, MMP3 and MMP13 in the PBNPs and LIPUS groups slightly decreased, and Bcl2 increased slightly, while in the combination treatment group, the former was significantly decreased, and Bcl2 was Significantly increased. Conclusion: The PBNPs/LIPUS combination treatment increased protein levels of p-PI3K, p-Akt, and p-mTOR but decreased the protein levels of p-JINK and p-c-Jun, in vitro and vivo. The PBNPs/LIPUS combination treatment reduced cellular ROS, apoptosis, and matrix metalloproteinases (MMPs), as a consequence, alleviated articular cartilage damage in KOA. Moreover, the PBNPs/LIPUS combination treatment suppressed the JINK/c-Jun signal pathway.
Prostate Cancer-Secreted Exosomal miR-1275 Modulates Osteoblast Proliferation and Differentiation by Targeting SIRT2/RUNX2 Cascade
Authors: Zou Zihao , Dai Ranran , Deng Nan , Su Wei , Liu Ping 
Published: September 2020

Abstract
BackgroundCancer-derived exosomes can promote tumor metastasis by delivering miRNAs from cancer cells to recipient cells. miR-1275 is suggested to be an important regulator involved in prostate cancer (PCa) metastasis; however, the association of miR-1275 with the microenvironment of PCa bone metastasis is unclear.Materials and methodsThe level of relative genes and proteins were detected by qPCR and western-blot, cell ability were examined by CCK-8 , the identification of target gene was analyzed by dual-luciferase reporter assay,exosomes were isolated from the PC3-derived conditioned medium by ultracentrifugation.Resultwe found that miR-1275 could be transferred from PCa cells to osteoblasts via exosomes. Exosomal miR-1275 significantly accelerated the proliferation of osteoblasts and the expression levels of osteoblast-specific genes, such as osteocalcin (OCN), type I collagen (COL-1), and osteopontin (OPN). Moreover, exosomal miR-1275 increased the expression of RUNX2, a master modulator of osteoblast differentiation, by down-regulation of SIRT2, which in turn influenced the proliferation and differentiation of osteoblasts.ConclusionsOur findings indicate that exosomal miR-1275 is a promoter of osteoblast activity, which may contribute to PCa bone metastasis.
Respiratory Rehabilitation After Blood Transfusion in a COVID-19 Patient: A Case Report
Authors: Mousavi Mohammad Javad , Obeidi Narges , keshmiri Saeed , Azodi Farzan , Kiyani Jamile , Abbasi Farhad 
Published: September 2020

Abstract
Background: The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been identified as the most crucial threat of the century. Due to severe pneumonia and acute respiratory distress syndrome (ARDS), the SARS-CoV-2 can cause shortness of breath, hypoxemia, and the need to mechanical ventilation, intensive care unit (ICU) management, and eventual death. We have tried to use a non-invasive approach to prevent patient from needing respiratory support with invasive ventilation (IV). Here, for the first time, improvement of oxygen delivery and oxygen saturation levels were observed in a COVID-19 patient using packed red blood cells (PRBCs) transfusion.Case presentation: A 63-year-old man with a history of smoking and addiction who came to our hospital facility with fever, shortness of breath and decreased blood oxygen saturation. High-resolution chest CT revealed bilateral and multifocal ground-glass opacities consistent with COVID-19. Subsequently, the COVID-19 infection was confirmed by real-time polymerase chain reaction (qRT-PCR) assay of the upper respiratory tract. Conclusions: Oxygen delivery and oxygen saturation improvement were observed in the COVID-19 patient, after PRBCs transfusions.
TÜRKİYE'DE HANEHALKI SORUMLUSUNUN TOPLAM GELİRİNE ETKİ EDEN BELİRLEYİCİLERİN KANTİL REGRESYON YÖNTEMİ İLE ANALİZİ
Authors: ÇOBANOĞLU Ferit , YILMAZ Halil İbrahim 
Published: September 2020

Bu çalışmada, Türkiye’de hanehalkı sorumlusunun
elde etmiş olduğu yıllık toplam gelirindeki farklılık, kantil regresyon yöntemi
ile analiz edilmiştir. Araştırmada, Türkiye İstatistik Kurumu (TÜİK) Başkanlığı'ndan
sağlanan “Hanehalkı Bütçe Araştırması 2015 yılı” mikro veri setinden yararlanılmıştır.
Çalışmada, hanehalkı sorumlusunun yıllık toplam geliri (TYG) bağımlı değişken
olarak alınırken, bağımsız değişkenler olarak ise hanehalkı sorumlusu için; cinsiyet
(C), yaş (Y) sağlık sigortasına sahip olma durumu (SS), günlük faaliyete engel
fiziksel ya da zihinsel problem olup-olmadığı (GFFZP), çalışmaya engel fiziksel
ya da zihinsel problem olup-olmadığı (CFZP), eğitim durumu (ED), medeni durum
(MD) ve çalışma durumu (CD) alınmıştır. Koşullu
medyan regresyonu analizine göre, hanehalkı sorumlusunun elde etmiş olduğu
yıllık toplam gelirinin medyanı 18880 TL olarak belirlenmiştir. Hanehalkı
sorumlusunun elde etmiş olduğu yıllık toplam gelirinin 25. yüzdebirlik değeri
12360 TL olarak belirlenirken, 75. yüzdebirlik değeri 30000 TL olarak
belirlenmiştir. Hanehalkı sorumlusunun elde etmiş olduğu yıllık toplam
gelirinin 5. yüzdebirlik değeri 3810 TL olarak hesaplanmış iken, 95.
yüzdebirlik değeri 60000 TL olarak belirlenmiştir. Sabit (robust) kantil
regresyon analizinde, bağımsız değişkenlerin hepsinin, TYG değişkeni üzerindeki
etkisinin istatiksel olarak önemli olduğu belirlenmiştir.
Subsistencia, dieta, salud y enfermedad en el pasado: del paradigma osteológico a la era molecular en Colombia
Authors: Corcione María Antonieta , Rodríguez Freddy 
Published: September 2020

En las últimas décadas, la bioarqueología colombiana ha tenido un desarrollo prominente tanto en la aplicación de nuevas técnicas como en la maduración de las conceptualizaciones teóricas en líneas temáticas sobre la subsistencia, la dieta y la salud, y enfermedad de las poblaciones prehispánicas que habitaron el territorio nacional. A diferentes escalas de análisis, los investigadores colombianos han resuelto interrogantes desde perspectivas multidisciplinares como la ecológica, evolutiva y biocultural utilizando cada vez más, técnicas radiológicas, histológicas y moleculares para la consecución de información. Este artículo de revisión tuvo como objetivo realizar un barrido de las investigaciones generadas en torno a los debates bioarqueológicos sobre adaptación al medio, migración y colonización de nuevos nichos, dieta, marcadores de actividad y condiciones de vida, principalmente. Sin que sea una exploración exhaustiva de la gran producción investigativa en el país, esta revisión ha permitido comprender los nuevos panoramas analíticos que se están generando entre los antropólogos de las diferentes regiones y de esta forma realizar una reflexión acerca de las nuevas sinergias que se promueven en la ciencia bioarqueológica colombiana.
El aporte de las colecciones osteológicas documentadas: líneas de investigación en la Colección “Prof. Dr. Rómulo Lambre” (La Plata, Argentina)
Authors: Plischuk Marcos , Garizoain Gonzalo , Petrone Selene , Desántolo Bárbara , García Mancuso Rocío , Salceda Susana , Inda Ana María 
Published: September 2020

Las colecciones osteológicas documentadas contribuyen a resolver problemas en la bioantropología desde su particular aporte a partir de la combinación de rasgos esqueléticos e información individual. Es por esto que posibilitan la generación de métodos estandarizados para la caracterización biológica de los individuos y permiten el análisis de características anatómicas, histológicas y patológicas desde una perspectiva poblacional, considerando su contexto socio-histórico. En el año 2005 en la Facultad de Ciencias Médicas (Universidad Nacional de La Plata, La Plata, Argentina), a fin de formar una colección documentada regional, se integró la Colección Osteológica Prof. Dr. R. Lambre a partir de restos esqueletéticos donados por el Cementerio Municipal La Plata (CMLP). Dichos restos provienen de cadáveres no reclamados, destinados a cremación u osario y exhumados por personal de la institución. La Colección está compuesta en la actualidad por 435 individuos con información documental asociada, (58,16% de sexo masculino y 41,84% de sexo femenino), con edades de muerte desde nonatos a 101 años, mientras que las fechas de muerte abarcan el período 1900-2003. Este artículo tiene como propósito describir las líneas de investigación desarrolladas en la colección Lambre desde su conformación y los principales resultados alcanzados. Para esto, se describen las tareas realizadas en relación con la caracterización biológica de individuos subadultos a partir de su esqueleto, se detallan los análisis histológicos en hueso y diente, principalmente enfocados en los métodos de estimación de edad de muerte, se comentan los estudios realizados en antropología dental, y finalmente, se describe el análisis osteopatológico. Se destacan tres aportes específicos hacia la bioantropología. En primer lugar, el aporte académico en la generación de conocimiento acerca de la variación histomorfológica del esqueleto durante la ontogenia. En segundo lugar, la formación de recursos humanos; el trabajo en la colección permitió la consolidación de un equipo interdisciplinario que se constituyó como formador de formadores en la investigación científica, docencia y extensión. Por último, y principalmente durante los últimos años, el incremento de transferencia de los resultados hacia organismos gubernamentales y no gubernamentales.
A Review on Catastrophic Evolution of SARS-CoV to SARS-CoV2: A Global Pandemic
Authors: Krishnan Sushmita , Subramanian Darshini , Rajamani Sri Sakthi Priyadarshini 
Published: September 2020

:
The coronaviruses, belonging to the family Coronaviridae, have caused a massive pandemic
in December 2019 after their previous outbreaks as SARS-CoV and MERS. The outbreak is believed to
have originated from the seafood and live market in the Hubei province of China. The Rhinolophus
species are the natural hosts of this virus. This virus caused pneumonia and took away many lives before
it was recognized as the novel Coronavirus. Very little information is available about the biology
and nature of the novel Coronavirus. This article reviews multiple aspects encompassing its origin, epidemiology,
pathogenesis, symptoms, and the global statistics of spread. Acute respiratory distress syndrome
(ARDS) is the key symptom of this condition. Angiotensin-converting enzyme 2 (ACE2) helps
in the penetration of the virus into the target cells. Deeper research and understanding are essential for
the identification of antibodies that inhibit ACE2 and can prevent viral replication. Drug design and
control of disease are crucial. In countries like India, where plant diversity is extensive, it is prudent to
focus on plant-based alternative drugs. Many attempts have been made to review and curate the drug
discovery attempts using immuno-informatic and bioinformatic tools.
Developing a Blockchain-Based Supply Chain System for Advanced Therapies: Protocol for a Feasibility Study
Authors: Lam Ching , van Velthoven Michelle Helena , Meinert Edward 
Published: September 2020

Background
Advanced therapies, including cell and gene therapies, have shown therapeutic promise in curing life-threatening diseases, such as leukemia and lymphoma. However, these therapies can be complicated and expensive to deliver due to their sensitivity to environment; troublesome tissue, cell, or genetic material sourcing; and complicated regulatory requirements.

Objective
This study aims to create a novel connected supply chain logistics and manufacturing management platform based on blockchain, with cell and gene therapy as a use case. Objectives are to define the requirements and perform feasibility evaluations on the use of blockchain for standardized manufacturing and establishment of a chain of custody for the needle-to-needle delivery of autologous cell and gene therapies. A way of lowering overall regulatory compliance costs for running a network of facilities operating similar or parallel processes will be evaluated by lowering the monitoring costs through publishing zero-knowledge proofs and product release by exception.

Methods
The study will use blockchain technologies to digitally connect and integrate supply chain with manufacturing to address the security, scheduling, and communication issues between advanced therapy treatment centers and manufacturing facilities in order to realize a transparent, secure, automated, and cost-effective solution to the delivery of these life-saving therapies. An agile software development methodology will be used to develop, implement, and evaluate the system. The system will adhere to the EU and US good manufacturing practices and regulatory requirements.

Results
This is a proposed study protocol, and upon acceptance, grant funding will be pursued for its execution in 2021.

Conclusions
The successful implementation of the integrated blockchain solution to supply chain and manufacturing of advanced therapies can push the industry standards toward a safer and more secure therapy delivery process.

International Registered Report Identifier (IRRID)
PRR1-10.2196/17005
STUDENTS’ KNOWLEDGE AND RISK OF COMMITTING MEDICATION ERRORS IN TWO SCHOOLS OF PHARMACY IN NIGERIA
Authors: CM UBAKA , C EKWE , A ISAH , AB MUKHTAR , CV UKWE 
Published: September 2020

Objective: The aim of this study was to determine the knowledge of prescription errors, the certainty of such knowledge, and the risk of committing medication errors among pharmacy students in two universities in Nigeria.
Methods: This study was a cross-sectional comparative survey between pharmacy students of two universities in Nigeria: University of Nigeria, Nsukka (UNN) and Nnamdi Azikiwe University (NAU), Awka. Study variables were measured using four simulated prescriptions and questionnaires. Chi-square test, independent t-test, and ordinal regression analyses were used to assess study outcomes.
Results: A total of 339 pharmacy students (239 in UNN and 100 in NAU), with a mean age of 24 (2.8) years and 57.2% (n=194) male students, participated in this study. Their accurate knowledge of each of the prescriptions were 294 (86.72%), 166 (48.97%), 199 (58.70%), and 248 (73.16%) for prescriptions with error of drug allergy, error of drug interaction, no error, and wrong dose of a drug, respectively. Students from UNN were more likely to commit a statistically significant high risk of error compared to NAU students in prescriptions with a drug allergy and wrong dose, while students in the 4th year class had a statistically significant higher odds of committing a drug interaction prescription error compared to final year students.
Conclusion: Pharmacy students evaluated in this study had good knowledge of medication error detection. The risk of these pharmacy students committing a prescription error was evident in nearly all prescriptions tested and the students’ school was the major predictor of these risks.
SPECTROPHOTOMETRIC DETERMINATION OF CEFPIROME IN PHARMACEUTICAL PREPARATION
Authors: CHAFLE DILIP M 
Published: September 2020

Objective: A simple, sensitive and precise visible spectrophotometric method has been proposed for the determination of cefpirome (CFM) in pure and oral injectable dosage form.
Methods: A spectrophotometric method is based on the formation of stable red color product by oxidation of drugs by ferric nitrate and subsequent complexation with 1, 10 – phenanthroline with maximum absorption at 515 nm.
Result: The red color complex was formed between Fe (II) and 1, 10 – phenanthroline after reduction of Fe (III) to Fe (II) in the presence of CFM drug. The phosphoric acid solution was used only for quenching the complex formation reaction. Several parameters such as the maximum wavelength of absorption, the volume of reagents, sequence of addition and effect of temperature and time of heating were optimized to achieve high sensitivity, stability and reproducible results. Under the optimum conditions, linear relationship with good correlation coefficient (0.994) was found over the concentration range from 0.20 to 6.00 μg/mL with a molar extinction coefficient 7.7813 × 104 L/mol/cm, limit of detection 0.2026 and limit of quantification 0.6141 μg/mL, respectively.
Conclusion: The proposed method was evaluated statistically for linearity, accuracy, and precision in terms of standard deviation, percentage recovery, percentage error and relative standard deviation. The proposed method can be applied for the routine estimation of CFM in the laboratory.
ANTIMICROBIAL POTENTIALS OF SOIL FUNGI METABOLITES, MOLECULAR DYNAMICS AND THEIR 3D PROTEIN STRUCTURAL PREDICTION USING BIOINFORMATICS TOOL
Authors: O. N. MAJOLAGBE , D. A. AINA , I. O. OMOMOWO , A. THOMAS 
Published: September 2020

Objective: To determine the antimicrobial potentials of secondary metabolite of soil fungi and predict their 3D structure and molecular identity.
Methods: Pure soil fungi were isolated from soil samples and cultured under submerged fermentation (Smf) for their metabolites using Potato Dextrose Agar and Broth. The secondary metabolites of the isolated fungi were obtained intracellularly after 21 d of incubation in a rotary shaker incubator. The antimicrobial potentials of the metabolites were investigated against four (4) clinical isolates, namely: Staphylococcus aureus, Klebsiella spp, Candida albicans and Escherichia coli. These soil fungi were further characterized to the molecular level and their evolutionary relationships established using bioinformatics tools. Protein structure of each of the fungi isolates was predicted using PHYRE-2.
Results: Out of all the soil fungi isolated, the metabolite of Aspergillus aculeatus showed the highest antimicrobial activities against Staphylococcus aureus (23.00±2.34 mm), Escherichia coli (9.00±1.44 mm) and Klebsiella spp (24.00±3.45 mm). The 3D protein structure predicted showed that each of the organisms consists of different amino-acid compositions such as: serine, tyrosine, proline, arginine, glycine, phenylalanine leucine with other notable biological properties.
Conclusion: The work revealed that secondary metabolites of the isolated fungi carry an important role in combating infectious agents thereby, providing roadmaps for the biosynthesis of many synthetic and semi-synthetic drugs and bio-products which are environmentally friendly.