bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transforming family dynamics: unexpected positive effects of treatment for adolescent gaming problems on family relationships
Authors: Kapetanovic Sabina , Due Nielsen Maiken , Gurdal Sevtap , Claesdotter-Knutsson Emma 
Published: December 2024

IntroductionWhile parenting is important for the development of adolescent problem gaming, it is unknown whether treatment of such problems in adolescents has a bearing on parenting. This study aims to explore the effects of individual relapse prevention (RP) treatment for adolescent problematic gaming on parenting practices and family dynamics.MethodsA total of 72 adolescents (74% male; Mage = 14.5 SD = 1.4), participated in the study, with n = 39 in the intervention group and n = 33 in the control group. Pre- and post-treatment data were collected on parenting practices (e.g., monitoring and communication) and family dynamics. Changes in parenting measures were analyzed using the Wilcoxon signed-rank test and Svensson's method of change analysis.ResultsAdolescents in the intervention group reported reduced family conflict and changes in specific parenting practices, including less inquiry, fewer rules and intrusion regarding gaming post-treatment. In contrast, the control group reported a decline in family cohesion at post-treatment assessment.DiscussionThe results highlight adolescents' active role in shaping family dynamics and underscore the positive spillover effects of treating adolescent problematic gaming on parenting practices and family relationships. These findings are relevant to clinical practice and deepen our understanding of the relationship between adolescent gaming, parent-child interactions, and treatment spillover effects.Trial registration numberClinicalTrials.gov, NCT05506384, https://clinicaltrials.gov/ct2/show/NCT05506384.
Durable and deep response to CVD chemotherapy in SDHB-mutated metastatic paraganglioma: case report
Authors: Zhang Chenyan , Wei Yuanfeng , Cheng Ke , Cao Dan 
Published: December 2024

IntroductionSuccinate dehydrogenase subunit B (SDHB)-mutated paragangliomas (PGLs) are rare neuroendocrine tumors characterized by increased malignancy, readily metastasizing, and poorer prognosis. Here we report a case of SDHB-mutated metastatic PGL, wherein the patient showed significant tumor shrinkage and complete symptom remission following chemotherapy. We aim to contribute additional evidence to the existing knowledge associated with SDHB-mutated PGLs.Case reportA 40-year-old male patient presented with recurrent hypoglycemia and hypertension crisis. Imaging revealed a huge left retroperitoneal tumor and multiple diffuse metastases in lungs. Catecholamine was also elevated, aligning with a diagnosis of metastatic PGL. Pathology also confirmed this diagnosis. Additionally, the immunohistochemistry indicated negative expression of SDHB and gene test showed somatic SDHB mutation. Given the SDHB mutation, cyclophosphamide-vincristine-dacarbazine (CVD) chemotherapy was initiated in critical conditions. Subsequently, a significant tumor shrinkage and complete biochemical response were observed after two treatment cycles. In September 2024, CT scan revealed new pulmonary lesions. The progression-free survival (PFS) with CVD chemotherapy was 24 months.ConclusionThis report reviews the distinct clinical and biochemical characteristics and treatment approaches of SDHB-mutated paragangliomas, emphasizing that the significance of incorporating both genetic testing and immunohistochemical analysis in clinical practice.
Identification of hub programmed cell death-related genes and immune infiltration in Crohn’s disease using bioinformatics
Authors: Wang Biyao , Liu Hailing , Guo Qin , Gao Xiang , Chao Kang , Yang Qingfan 
Published: December 2024

BackgroundCrohn’s disease (CD) is an immune-mediated disorder characterized by immune cell infiltration that induces persistent chronic inflammation of the gastrointestinal tract. Programmed cell death (PCD) plays a critical role in the pathogenesis of CD. This study identified vital PCD-related genes in CD based on immune infiltration using bioinformatic analysis.MethodsWe obtained two CD datasets from the Gene Expression Omnibus (GEO) database and examined immune cell infiltration to investigate immune cell dysregulation in CD. PCD-related genes were retrieved from the GeneCards database. Based on the differentially expressed genes (DEGs) and PCD gene sets, PCD-related DEGs were identified. Candidate hub genes were identified using a protein-protein interaction (PPI) network, and their diagnostic effectiveness was predicted using receiver operating characteristic (ROC) curve analysis. Functional enrichment and immune infiltration analyses were used to assess the distinct roles of the hub genes. Finally, the miRWalk and ENCORI databases were used to predict which microRNAs (miRNAs) regulated the hub genes and to verify gene expression in CD colonic tissues via transcriptome sequencing.ResultsA total of 335 PCD-related DEGs and 3 hub genes (MMP1, SAA1, and PLAU) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analyses indicated the enrichment of these genes in the immune response. Infiltration analysis of immune cells showed abundant endothelial cells, plasma cells, dendritic cells, and monocytes in the CD samples. Based on the correlation analysis, the three hub genes were positively correlated with monocytes and negatively correlated with CD8 naïve T-cells. MMP1, SAA1, and PLAU correlated with the pathogenicity of CD and had good diagnostic value for CD. The three hub genes were highly expressed in the CD tissues, as confirmed using transcriptome sequencing.ConclusionThis study identified MMP1, SAA1, and PLAU as hub genes involved in PCD in patients with CD. These genes regulate immune cell function and their expression levels are closely related to immune cell infiltration. These findings provide novel insights into the mechanisms underlying CD pathogenesis. The identified PCD genes and regulatory miRNAs are potential biomarkers and therapeutic targets for CD.
Assessing the causal relationships between circulating metabolic biomarkers and breast cancer by using mendelian randomization
Authors: Wang Bowen , Ling Yue , Zhang Hui , Yang Ming 
Published: December 2024

ObjectivePrevious studies have established a causal relationship between metabolites and breast cancer (BC), but the underlying mechanisms remain unclear. Thus, we aimed to investigate the genetic relationship between metabolites and BC, including its subtypes, using Mendelian randomization (MR) analysis.MethodsUtilizing the latest and most comprehensive summary statistics from genome-wide association studies we conducted an Mendelian randomization study. Data on 233 metabolites, used as exposure variables, were obtained from a study involving 136,016 participants. BC data, used as outcome variables, were sourced from a study comprising 122,977 cases and 105,974 controls. We used the inverse-variance weighted method as the primary approach, along with three supplementary methods, to assess the causal relationship. We also used Cochran’s Q test to detect heterogeneity and MR-Egger regression to examine the presence of horizontal pleiotropy.ResultsUpon analyzing 233 metabolites across 11 classes in relation to BC, we found six classes of metabolites (fatty acids glycerides and phospholipids, lipoprotein subclasses, lipids, apolipoproteins, and lipoprotein particle size) associated with overall BC. Five classes of metabolites (fatty acids glycerides and phospholipids, lipoprotein subclasses, lipids, and lipoprotein particle size) were related to estrogen receptor (ER) + BC, and eight classes of metabolites (fatty acids, amino acids, glycerides and phospholipids, lipoprotein subclasses, lipids, apolipoproteins, glycolysis-related metabolites, and lipoprotein particle size) were linked to ER- BC.ConclusionOur study demonstrates a genetic causal relationship between most metabolites and BC, confirming the link between these factors. This research provides a significant foundation for the prevention and treatment of BC.
Exercise preconditioning and resveratrol reduce the susceptibility of rats with obstructive jaundice to endotoxin and alleviate lung injury
Authors: Li Xujiong , Li Wei , Wang Tao , Li Yan , Zhao Li 
Published: December 2024

IntroductionEndotoxemia is a common issue for patients with biliary obstruction. The lung is the most affected organ by endotoxins. Exercise training can alleviate lipopolysaccharide (LPS)-induced lung inflammation and resveratrol has biological effects similar to exercise. In this study, we evaluated the protective effects of exercise preconditioning, resveratrol, and their combination on LPS-induced lung injury and mortality in rats with obstructive jaundice.MethodsEndotoxemia was simulated in rats by common bile duct ligation (CBDL) and intraperitoneal injection of low-dose LPS. The treatment groups were pretreated with exercise and/or resveratrol to assess their effects on lung injury and mortality. Immunohistochemistry, immunofluorescence, and ELISA were subsequently used to evaluate the impact of exercise and/or resveratrol on inflammation in lung tissue and bronchoalveolar lavage fluid.ResultsWe found that even in the early stages, compared to sham-LPS rats, low-dose LPS induced excessive systemic inflammatory responses in CBDL rats, as evidenced by a significant increase in TNF-α and IL-6, severe lung inflammation, lung injury, and higher mortality rates, indicating that cholestasis increased rats’ susceptibility to endotoxins. Exercise training reduced neutrophil infiltration in the lungs of model rats and IL-6 levels in bronchoalveolar lavage fluid. Both exercise training and resveratrol exhibited synergistic effects in reducing macrophage accumulation in lung tissues, lowering TNF-α and IL-6 levels in the lungs, and decreasing TNF-α concentration in bronchoalveolar lavage fluid. Additionally, exercise and combined interventions both significantly increased the expression of IL-10. The interventions induced a marked improvement in lung tissue pathological damage and lung edema in model rats and prolonged the survival time of rats with obstructive jaundice.DiscussionThis study demonstrates that exercise preconditioning and/or resveratrol can significantly reduce rats’ susceptibility to endotoxins after CBDL and alleviate lung injuries through their anti-inflammatory effects, thereby decreasing the mortality risk.
Diagnostic value of multi-tumor-associated autoantibody expression in esophageal squamous cell carcinoma and correlation of clinical features
Authors: Zhou Sihao , Liu Kejun , Yang Yuyu , Yuan Chuan , Liu Yi , Liang Yi , Li Wenhao , Zhang Jingjing , Ye Hongyu , Gong Sheng , Wu Yingmeng , Huang Weizhao 
Published: December 2024

ObjectiveThis study aimed to investigate the diagnostic value of 7-tumor associated autoantibodies (7-TAAB) and to evaluate the relationship between 7-TAAB and clinical features in esophageal squamous cell carcinoma (ESCC), which can be used to guide clinical diagnosis and treatment and achieve its clinical value.Methods(1) Blood specimens were collected from patients with ESCC who had not previously received antitumor therapy (ESCC group) and those who had normal medical check-ups in the hospital during the same period (control group). The concentrations of 7-TAAB (P53, PGP9.5, SOX2, GAGE7, GBU4-5, MAGE A1, and CAGE) in serum were determined by enzyme-linked immunosorbent assay. The concentrations of 7-TAAB were compared between the ESCC and control groups, and the positive rate of 7-TAAB was calculated to determine the sensitivity, specificity, and accuracy of 7-TAAB. The diagnostic value of 7-TAAB was analyzed using the receiver operating characteristic (ROC) curve. (2) The clinical data of patients with ESCC were collected and the correlation between the rate of 7-TAAB and clinical features was analyzed.Results(1) The serum levels and positivity rates of five antibodies (PGP9.5, SOX2, GBU4-5, MAGE-A1, and CAGE) were higher in the ESCC group than in the control group (P &lt; 0.05) and the positive expression rate of the combined serum 7-TAAB in the ESCC group was significantly higher than that in the control group (P &lt; 0.05). (2) The sensitivity of single antibody detection was 4.20%–17.65%, with a specificity of 96.49%–100%, and accuracy of 51.07%–57.94%. The sensitivity of 7-TAAB combined detection was 49.58%, the specificity was 92.98%, and the accuracy was 70.81%. (3) The ROC curve showed that the 7-TAAB combined test had a certain diagnostic value for ESCC and that its diagnostic efficacy was significantly higher than that of the single autoantibody tests. The diagnostic efficacy of the combined test with the remaining five antibodies (PGP9.5, SOX2, GBU4-5, MAGE-A1, and CAGE) was similar to that of the 7-TAAB combined test after eliminating the two antibodies with low expression rates. (4) Univariate analysis revealed significant differences in the positive expression rates of the 7-TAAB combination test in terms of age, hemoglobin level, albumin level, tumor location, tumor length, lymph node stage, and tumor clinical stage (P &lt; 0.05), and multivariate analysis revealed that age and lymph node stage were independent factors affecting antibody expression.ConclusionThe multi-tumor-associated autoantibody combination test not only has a good auxiliary diagnostic value but also closely correlates with the clinical features of ESCC.
Pseudomonas oryzihabitans D1-104/3 and P. gessardii C31-106/3 differentially modulate the antioxidative response of duckweed (Lemna minor L.) to salt stress
Authors: Popržen Tatjana , Antonić Reljin Dragana , Uzelac Branka , Milovančević Marija , Paunović Danijela , Trifunović-Momčilov Milana , Marković Marija , Raspor Martin , Nikolić Ivan , Stanković Slaviša , Radulović Olga 
Published: December 2024

IntroductionThe common duckweed (L. minor) is a model organism for investigation of plant physiology, especially stress-related responses. Its two physiological characteristics are of special interest: (1) salt-stressed duckweeds may accumulate starch, a precursor for biofuel; (2) duckweeds are associated with various beneficial (plant-growth promoting, PGP) bacterial strains. In this paper, we analyzed the role of two bacterial strains: Pseudomonas oryzihabitans D1-104/3 and P. gessardii C31-106/3 in regulation of duckweed's growth and antioxidative responses to salt (10 and 100 mM NaCl) and hypothesized that they alleviate salt-induced oxidative stress.MethodsFresh and dry weight, frond number, photosynthetic pigments, malondialdehyde (MDA) and hydrogen peroxide (H2O2), ascorbic acid (AsA), proline, total polyphenol (TPC) and starch content, as well as antioxidant capacity and antioxidant enzymatic activity were measured after 14 days. Fluorescence microscopy was used to visualize bacterial presence on duckweeds.ResultsFluorescence microscope revealed that Pseudomonas bacteria colonized all duckweed surfaces. The doubling time of duckweeds inoculated with P. gessardii C31-106/3 was significantly longer. Additionally, at 0 and 10 mM NaCl, this strain decreased chlorosis in duckweeds. Moreover, P. gessardii C31-106/3 increased dry-to-fresh-weight ratio, proline, chlorophyll a, b and carotenoid content at 100 mM, as well as AsA content in plants in NaCl-free medium, while P. oryzihabitans D1-104/3 increased AsA at 100 mM NaCl. Both bacterial strains decreased lipid peroxidation, while P. gessardii C31-106/3 increased and P. oryzihabitans D1-104/3 decreased H2O2 content at 100 mM and 0 mM NaCl, respectively. Bacteria significantly increased TPC and antioxidant capacity at 100 mM NaCl, particularly P. oryzihabitans D1-104/3. After 14 days, the SOD and POX activities were at the same level in all samples. At 100 mM NaCl, CAT activity was increased in all plants.DiscussionThe results of this study show that two Pseudomonas strains had markedly different effects on duckweed: while P. oryzihabitans D1-104/3 supported growth, P. gessardii C31-106/3 prioritized salt stress tolerance in duckweeds.
The impact of interactions between heavy metals and smoking exposures on the formation of oral microbial communities
Authors: Zheng Qiwen , Zhang Yonghua , Li Jia , Pei Shuwei , Liu Jiangyun , Feng Lu , Zhang Li , Liu Xingrong , Luo Bin , Ruan Ye , Hu Weigang , Niu Jingping , Tian Tian 
Published: December 2024

IntroductionThe primary objective of our investigation was to assess the repercussions of prolonged exposure to heavy metals and smoking on the microbiome of the oral buccal mucosa. Concurrently, we aimed to elucidate the intricate interplay between external environmental exposures and the composition of the oral microbial ecosystem, thereby discerning its potential implications for human health.MethodsOur study cohort was stratified into four distinct groups: MS (characterized by concurrent exposure to heavy metals and smoking), M (exposed solely to heavy metals), S (exposed solely to smoking), and C (comprising individuals serving as a control group). Specimens of buccal mucosa and blood were systematically acquired from the participants, facilitating subsequent microbial diversity analysis across the four oral buccal mucosa sample cohorts through 16S rRNA gene sequencing techniques. Simultaneously, blood samples were tested for heavy metal concentrations. In addition, we performed topological analyses by constructing microbial networks.ResultsOur findings notably indicate that co-exposure to heavy metals and smoking yielded a more pronounced alteration in the diversity of oral microflora when compared to singular exposures to either heavy metals or smoking. By comparing the oral bacterial communities and functional pathways between the four groups, we found significant differences in bacterial communities and functional pathways between the groups. Notably, the impact of heavy metal exposure overshadowed that of smoking, with concurrent exposure to heavy metals and smoking eliciting marginally greater effects than exposure to heavy metals alone. In addition, our analysis of the correlation between microbiota and blood heavy metal concentrations showed that the heavy metal cadmium (Cd) had a significantly greater effect on oral microbiota than other heavy metals.DiscussionChronic exposure to heavy metals and smoking disrupts the normal bacterial communities in the oral mucosa of residents of contaminated areas. This exposure reduces the complexity and stability of microbial networks and increases the risk of various diseases reduces the complexity and stability.
TP53 oncogenic variants as prognostic factors in individuals with glioblastoma: a systematic review and meta-analysis
Authors: Esperante Diego , Galicia Kena Daza , Rivas-Cuervo Kalu Gabriel , Cacho-Díaz Bernardo , Trejo-Becerril Catalina , Taja-Chayeb Lucia , Aboud Orwa , Carlos-Escalante José Alberto , Wegman-Ostrosky Talia 
Published: December 2024

BackgroundThis systematic review and meta-analysis investigated the relationship between somatic TP53 oncogenic variants and prognosis, specifically with overall survival (OS) and progression-free survival (PFS) in patients diagnosed with supratentorial glioblastoma.MethodsWe included longitudinal studies and clinical trials involving a minimum of 40 adult participants diagnosed with supratentorial glioblastoma, wherein the status of TP53 variants was assessed. We conducted searches in multiple databases. We assessed bias risk using a modified version of the Quality in Prognosis Studies tool, and the certainty of evidence was evaluated following the principles of the GRADE approach.Results and conclusionThis study encompassed 23 papers involving 2,555 patients, out of which 716 had reported oncogenic variants. TP53 oncogenic variants were associated with a reduced likelihood of 1-year survival (OR 0.52, 95% CI 0.29–0.94). However, our analysis did not reveal any significant impact of TP53 variants on overall survival, progression-free survival, or 2-year survival. Therefore, this comprehensive analysis demonstrates that the presence of genetic variants in TP53 does not provide useful information for the prognosis of glioblastoma.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42021289496.
Feasibility of large language models for CEUS LI-RADS categorization of small liver nodules in patients at risk for hepatocellular carcinoma
Authors: Huang Jiayan , Yang Rui , Huang Xiaotong , Zeng Keyu , Liu Yan , Luo Jun , Lyshchik Andrej , Lu Qiang 
Published: December 2024

BackgroundLarge language models (LLMs) offer opportunities to enhance radiological applications, but their performance in handling complex tasks remains insufficiently investigatedPurposeTo evaluate the performance of LLMs integrated with Contrast-enhanced Ultrasound Liver Imaging Reporting and Data System (CEUS LI-RADS) in diagnosing small (≤20mm) hepatocellular carcinoma (sHCC) in high-risk patients.Materials and MethodsFrom November 2014 to December 2023, high-risk HCC patients with untreated small (≤20mm) focal liver lesions (sFLLs), were included in this retrospective study. ChatGPT-4.0, ChatGPT-4o, ChatGPT-4o mini, and Google Gemini were integrated with imaging features from structured CEUS LI-RADS reports to assess their diagnostic performance for sHCC. The diagnostic efficacy of LLMs for small HCC were compared using McNemar test.ResultsThe final population consisted of 403 high-risk patients (52 years ± 11, 323 men). ChatGPT-4.0 and ChatGPT-4o demonstrated substantial to almost perfect intra-agreement for CEUS LI-RADS categorization (κ values: 0.76-1.0 and 0.7-0.94, respectively), outperforming ChatGPT-4o mini (κ values: 0.51-0.72) and Google Gemini (κ values: -0.04-0.47). ChatGPT-4.0 had higher sensitivity in detecting sHCC than ChatGPT-4o (83%-89% vs. 70%-78%, p &lt; 0.02) with comparable specificity (76%-90% vs. 83%-86%, p &gt; 0.05). Compared to human readers, ChatGPT-4.0 showed superior sensitivity (83%-89% vs. 63%-78%, p &lt; 0.004) and comparable specificity (76%-90% vs. 90%-95%, p &gt; 0.05) in diagnosing sHCC.ConclusionLLM integrated with CEUS LI-RADS offers potential tool in diagnosing sHCC for high-risk patients. ChatGPT-4.0 demonstrated satisfactory consistency in CEUS LI-RADS categorization, offering higher sensitivity in diagnosing sHCC while maintaining comparable specificity to that of human readers.
Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm
Authors: Miao Yang , Shuang Wang , Qianwei Qu , Xin Liu , Wei Peng , Hai Yang , Yonghui Zhou , Xinbo Yu 
Published: December 2024

IntroductionThe mechanism of tannic acid (TA) intervention on methicillin-resistant Staphylococcus aureus (MRSA, USA 300) biofilm formation was explored using proteomics.MethodsThe minimum inhibitory concentration (MIC) of TA against the MRSA standard strain USA 300 was determined by two-fold serial dilution of the microbroth. The effects of TA were studied using crystal violet staining. The morphology of TA-treated USA 300 cells was observed by scanning electron microscopy and confocal laser scanning microscopy. Differentially expressed proteins (DEPs) were screened using proteomic and biological information analyses, and their transcriptional levels were verified using real-time quantitative polymerase chain reaction.ResultsThe MIC of TA was 0.625 mg/mL, whereas 1/2 MIC (0.3125 mg/mL) of TA significantly inhibited biofilm formation without affecting the bacterial growth (p &lt; 0.01) and prevented the formation of a complete three-dimensional biofilm structure. Using 1/2 MIC of TA, 208 DEPs were identified, of which 127 were upregulated and 81 were downregulated. The transcriptional levels of the genes corresponding to five randomly selected DEPs (glnA, ribD, clpB, gap, and lukE) were consistent with the proteomics data (p &lt; 0.05). Bioinformatic analysis showed that the changes in the MRSA strains after TA intervention primarily involved pyrimidine and purine metabolisms, arginine biosynthesis, and the citric acid cycle.ConclusionTA exerts an antibacterial effect on MRSA and can be used as a potential candidate for the development of anti-biofilm drugs, thereby laying a foundation for the treatment of MRSA biofilm-induced infections.