bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compression and stretch sensitive submucosal neurons of the porcine and human colon
Authors: Filzmayer Anna Katharina , Elfers Kristin , Michel Klaus , Buhner Sabine , Zeller Florian , Demir Ihsan Ekin , Theisen Jörg , Schemann Michael , Mazzuoli-Weber Gemma 
Published: August 2020

AbstractThe pig is commonly believed to be a relevant model for human gut functions—however, there are only a few comparative studies and none on neural control mechanisms. To address this lack we identified as one central aspect mechanosensitive enteric neurons (MEN) in porcine and human colon. We used neuroimaging techniques to record responses to tensile or compressive forces in submucous neurons. Compression and stretch caused Ca-transients and immediate spike discharge in 5–11% of porcine and 15–24% of human enteric neurons. The majority of these MEN exclusively responded to either stimulus quality but about 9% responded to both. Most of the MEN expressed choline acetyltransferase and substance P; nitric oxide synthase-positive MEN primarily occurred in distal colon. The findings reveal common features of MEN in human and pig colon which we interpret as a result of species-independent evolutionary conservation rather than a specific functional proximity between the two species.
Effect of kaempferol on the transgenic Drosophila model of Parkinson’s disease
Authors: Rahul  , Naz Falaq , Jyoti Smita , Siddique Yasir Hasan 
Published: August 2020

AbstractThe present study was aimed to study the effect of kaempferol, on the transgenic Drosophila model of Parkinson’s disease. Kaempferol was added in the diet at final concentration of 10, 20, 30 and 40 µM and the effect was studied on various cognitive and oxidative stress markers. The results of the study showed that kaempferol, delayed the loss of climbing ability as well as the activity of PD flies in a dose dependent manner compared to unexposed PD flies. A dose-dependent reduction in oxidative stress markers was also observed. Histopathological examination of fly brains using anti-tyrosine hydroxylase immunostaining has revealed a significant dose-dependent increase in the expression of tyrosine hydroxylase in PD flies exposed to kaempferol. Molecular docking results revealed that kaempferol binds to human alpha synuclein at specific sites that might results in the inhibition of alpha synuclein aggregation and prevents the formation of Lewy bodies.
Morphological landscape of endothelial cell networks reveals a functional role of glutamate receptors in angiogenesis
Authors: Sailem Heba Z. , Al Haj Zen Ayman 
Published: August 2020

AbstractAngiogenesis plays a key role in several diseases including cancer, ischemic vascular disease, and Alzheimer’s disease. Chemical genetic screening of endothelial tube formation provides a robust approach for identifying signalling components that impact microvascular network morphology as well as endothelial cell biology. However, the analysis of the resulting imaging datasets has been limited to a few phenotypic features such as the total tube length or the number of branching points. Here we developed a high content analysis framework for detailed quantification of various aspects of network morphology including network complexity, symmetry and topology. By applying our approach to a high content screen of 1,280 characterised drugs, we found that drugs that result in a similar phenotype share the same mechanism of action or common downstream signalling pathways. Our multiparametric analysis revealed that a group of glutamate receptor antagonists enhances branching and network connectivity. Using an integrative meta-analysis approach, we validated the link between these receptors and angiogenesis. We further found that the expression of these genes is associated with the prognosis of Alzheimer’s patients. In conclusion, our work shows that detailed image analysis of complex endothelial phenotypes can reveal new insights into biological mechanisms modulating the morphogenesis of endothelial networks and identify potential therapeutics for angiogenesis-related diseases.
Campylobacter infections expected to increase due to climate change in Northern Europe
Authors: Kuhn Katrin Gaardbo , Nygård Karin Maria , Guzman-Herrador Bernardo , Sunde Linda Selje , Rimhanen-Finne Ruska , Trönnberg Linda , Jepsen Martin Rudbeck , Ruuhela Reija , Wong Wai Kwok , Ethelberg Steen 
Published: August 2020

AbstractGlobal climate change is predicted to alter precipitation and temperature patterns across the world, affecting a range of infectious diseases and particularly foodborne infections such as Campylobacter. In this study, we used national surveillance data to analyse the relationship between climate and campylobacteriosis in Denmark, Finland, Norway and Sweden and estimate the impact of climate changes on future disease patterns. We show that Campylobacter incidences are linked to increases in temperature and especially precipitation in the week before illness, suggesting a non-food transmission route. These four countries may experience a doubling of Campylobacter cases by the end of the 2080s, corresponding to around 6,000 excess cases per year caused only by climate changes. Considering the strong worldwide burden of campylobacteriosis, it is important to assess local and regional impacts of climate change in order to initiate timely public health management and adaptation strategies.
Analysis of small RNA populations generated in peanut leaves after exogenous application of dsRNA and dsDNA targeting aflatoxin synthesis genes
Authors: Power Imana L. , Faustinelli Paola C. , Orner Valerie A. , Sobolev Victor S. , Arias Renee S. 
Published: August 2020

AbstractPreviously, we have shown that RNA interference (RNAi) can prevent aflatoxin accumulation in transformed peanuts. To explore aflatoxin control by exogenous delivery of double-strand RNA (dsRNA) it is necessary to understand the generation of small RNA (sRNA) populations. We sequenced 12 duplicate sRNA libraries of in-vitro-grown peanut plants, 24 and 48 h after exogenous application of five gene fragments (RNAi-5x) related to aflatoxin biosynthesis in Aspergillus flavus. RNAi-5x was applied either as double-stranded RNA (dsRNA) or RNAi plasmid DNA (dsDNA). Small interfering RNAs (siRNAs) derived from RNAi-5x were significantly more abundant at 48 h than at 24 h, and the majority mapped to the fragment of aflatoxin efflux-pump gene. RNAi-5x-specific siRNAs were significantly, three to fivefold, more abundant in dsDNA than dsRNA treatments. Further examination of known micro RNAs related to disease-resistance, showed significant down-regulation of miR399 and up-regulation of miR482 in leaves treated with dsDNA compared to the control. These results show that sRNA sequencing is useful to compare exogenous RNAi delivery methods on peanut plants, and to analyze the efficacy of molecular constructs to generate siRNAs against specific gene targets. This work lays the foundation for non-transgenic delivery of RNAi in controlling aflatoxins in peanut.
Salt coatings functionalize inert membranes into high-performing filters against infectious respiratory diseases
Authors: Rubino Ilaria , Oh Euna , Han Sumin , Kaleem Sana , Hornig Alex , Lee Su-Hwa , Kang Hae-Ji , Lee Dong-Hun , Chu Ki-Back , Kumaran Surjith , Armstrong Sarah , Lalani Romani , Choudhry Shivanjali , Kim Chun Il , Quan Fu-Shi , Jeon Byeonghwa , Choi Hyo-Jick 
Published: August 2020

AbstractRespiratory protection is key in infection prevention of airborne diseases, as highlighted by the COVID-19 pandemic for instance. Conventional technologies have several drawbacks (i.e., cross-infection risk, filtration efficiency improvements limited by difficulty in breathing, and no safe reusability), which have yet to be addressed in a single device. Here, we report the development of a filter overcoming the major technical challenges of respiratory protective devices. Large-pore membranes, offering high breathability but low bacteria capture, were functionalized to have a uniform salt layer on the fibers. The salt-functionalized membranes achieved high filtration efficiency as opposed to the bare membrane, with differences of up to 48%, while maintaining high breathability (> 60% increase compared to commercial surgical masks even for the thickest salt filters tested). The salt-functionalized filters quickly killed Gram-positive and Gram-negative bacteria aerosols in vitro, with CFU reductions observed as early as within 5 min, and in vivo by causing structural damage due to salt recrystallization. The salt coatings retained the pathogen inactivation capability at harsh environmental conditions (37 °C and a relative humidity of 70%, 80% and 90%). Combination of these properties in one filter will lead to the production of an effective device, comprehensibly mitigating infection transmission globally.
Redox-active, luminescent coordination nanosheet capsules containing magnetite
Authors: Arai Ryo , Li Mengjuan , Toyoda Ryojun , Maeda Hiroaki , Nishihara Hiroshi 
Published: August 2020

AbstractTwo-dimensional coordination nanosheets (CONASHs) are grown at the spherical liquid–liquid interface of a dichloromethane droplet in water to form zero-dimensional nano- and micro-capsules using a simple dropping method, a syringe-pump method, and an emulsion method. Reaction of 1,3,5-tris[4-(4′-2,2′:6′,2″-terpyridyl)phenyl]benzene (1) with Fe(BF4)2 affords electrochromic Fe(tpy)2 CONASH capsules and that of ligand 1 with ZnSO4 does photoluminescent Zn2(μ-O2SO2)2(tpy)2 CONASH capsules. Fe(tpy)2 CONASH capsules containing magnetite particles were produced by the syringe-pump method by adding magnetite to the aqueous phase, with the assembly and dispersion of the magnetite-containing CONASH capsules being easily controlled with a magnet. This indicates that physicochemically functional CONASH capsules are suitable for incorporating other functional materials to develop hybrid systems.
Spectral dependence of third-order susceptibility of Au triangular nanoplates
Authors: Zhang Boyi , Sato Rodrigo , Tanaka Miyoko , Takeda Yoshihiko 
Published: August 2020

AbstractWe experimentally investigated the spectral dependence of the third-order susceptibility $$\chi^{\left( 3 \right)}$$

χ

3

of Au triangular nanoplates in a broad wavelength region (400–1,000 nm). Complex shaped plasmonic nanoparticles provide a promising route to achieve control of their optical properties at the nanoscale. However, little is known about the effects of geometrical parameters to the optical nonlinearities and underlying mechanisms of the plasmon modes. Here, we obtained the $$\chi^{\left( 3 \right)}$$

χ

3

of Au triangular nanoplates featuring a narrow plasmon resonance that is tunable in the visible and near-IR regions. This work demonstrates that the plasmonic triangular nanoplates simultaneously shows self-focusing and -defocusing, and saturable and reverse-saturable absorption properties at specific wavelength regions. Maximum amplitudes of real and imaginary components are − 6.8 × 10−18 m2/V2 at 668 nm and − 6.7 × 10−18 m2/V2 at 646 nm, respectively. Spectral dependence of the quantity $$\chi^{\left( 3 \right)}$$

χ

3

enables comparison between different shaped plasmonic NPs to boost active plasmonic applications performance.
Versatile formation of supported lipid bilayers from bicellar mixtures of phospholipids and capric acid
Authors: Sut Tun Naw , Yoon Bo Kyeong , Park Soohyun , Jackman Joshua A. , Cho Nam-Joon 
Published: August 2020

AbstractOriginally developed for the structural biology field, lipid bicelle nanostructures composed of long- and short-chain phospholipid molecules have emerged as a useful interfacial science tool to fabricate two-dimensional supported lipid bilayers (SLBs) on hydrophilic surfaces due to ease of sample preparation, scalability, and versatility. To improve SLB fabrication prospects, there has been recent interest in replacing the synthetic, short-chain phospholipid component of bicellar mixtures with naturally abundant fatty acids and monoglycerides, i.e., lauric acid and monocaprin. Such options have proven successful under specific conditions, however, there is room for devising more versatile fabrication options, especially in terms of overcoming lipid concentration-dependent SLB formation limitations. Herein, we investigated SLB fabrication by using bicellar mixtures consisting of long-chain phospholipid and capric acid, the latter of which has similar headgroup and chain length properties to lauric acid and monocaprin, respectively. Quartz crystal microbalance-dissipation, epifluorescence microscopy, and fluorescence recovery after photobleaching experiments were conducted to characterize lipid concentration-dependent bicelle adsorption onto silicon dioxide surfaces. We identified that uniform-phase SLB formation occurred independently of total lipid concentration when the ratio of long-chain phospholipid to capric acid molecules (“q-ratio”) was 0.25 or 2.5, which is superior to past results with lauric acid- and monocaprin-containing bicelles in which cases lipid concentration-dependent behavior was observed. Together, these findings demonstrate that capric acid-containing bicelles are versatile tools for SLB fabrication and highlight how the molecular structure of bicelle components can be rationally finetuned to modulate self-assembly processes at solid–liquid interfaces.
Decreased expression of the thyroid hormone-inactivating enzyme type 3 deiodinase is associated with lower survival rates in breast cancer
Authors: Goemann Iuri Martin , Marczyk Vicente Rodrigues , Recamonde-Mendoza Mariana , Wajner Simone Magagnin , Graudenz Marcia Silveira , Maia Ana Luiza 
Published: August 2020

AbstractThyroid hormones (THs) are critical regulators of cellular processes, while changes in their levels impact all the hallmarks of cancer. Disturbed expression of type 3 deiodinase (DIO3), the main TH-inactivating enzyme, occurs in several human neoplasms and has been associated with adverse outcomes. Here, we investigated the patterns of DIO3 expression and its prognostic significance in breast cancer. DIO3 expression was evaluated by immunohistochemistry in a primary cohort of patients with breast cancer and validated in a second cohort using RNA sequencing data from the TCGA database. DNA methylation data were obtained from the same database. DIO3 expression was present in normal and tumoral breast tissue. Low levels of DIO3 expression were associated with increased mortality in the primary cohort. Accordingly, lowDIO3mRNA levels were associated with an increased risk of death in a multivariate model in the validation cohort. DNA methylation analysis revealed that theDIO3gene promoter is hypermethylated in tumors when compared to normal tissue. In conclusion, DIO3 is expressed in normal and tumoral breast tissue, while decreased expression relates to poor overall survival in breast cancer patients. Finally, loss of DIO3 expression is associated with hypermethylation of the gene promoter and might have therapeutic implications.
Historical total ozone radiative forcing derived from CMIP6 simulations
Authors: Skeie Ragnhild Bieltvedt , Myhre Gunnar , Hodnebrog Øivind , Cameron-Smith Philip J. , Deushi Makoto , Hegglin Michaela I. , Horowitz Larry W. , Kramer Ryan J. , Michou Martine , Mills Michael J. , Olivié Dirk J. L. , Connor Fiona M. O’ , Paynter David , Samset Bjørn H. , Sellar Alistair , Shindell Drew , Takemura Toshihiko , Tilmes Simone , Wu Tongwen 
Published: August 2020

AbstractRadiative forcing (RF) time series for total ozone from 1850 up to the present day are calculated based on historical simulations of ozone from 10 climate models contributing to the Coupled Model Intercomparison Project Phase 6 (CMIP6). In addition, RF is calculated for ozone fields prepared as an input for CMIP6 models without chemistry schemes and from a chemical transport model simulation. A radiative kernel for ozone is constructed and used to derive the RF. The ozone RF in 2010 (2005–2014) relative to 1850 is 0.35 W m−2 [0.08–0.61] (5–95% uncertainty range) based on models with both tropospheric and stratospheric chemistry. One of these models has a negative present-day total ozone RF. Excluding this model, the present-day ozone RF increases to 0.39 W m−2 [0.27–0.51] (5–95% uncertainty range). The rest of the models have RF close to or stronger than the RF time series assessed by the Intergovernmental Panel on Climate Change in the fifth assessment report with the primary driver likely being the new precursor emissions used in CMIP6. The rapid adjustments beyond stratospheric temperature are estimated to be weak and thus the RF is a good measure of effective radiative forcing.
Neglecting irrigation contributes to the simulated summertime warm-and-dry bias in the central United States
Authors: Qian Yun , Yang Zhao , Feng Zhe , Liu Ying , Gustafson William I. , Berg Larry K. , Huang Maoyi , Yang Ben , Ma Hsi-Yen 
Published: August 2020

AbstractA vast number of weather forecast and climate models have a common warm-and-dry bias, accompanied by the underestimation of evapotranspiration and overestimation of surface net radiation, over the central United States during boreal summer. Various theories have been proposed to explain these biases, but no studies have linked the biases with the missing representation of human perturbations, such as irrigation. Here we argue that neglecting the impact of irrigation contributes to the longstanding warm surface temperature and lack of precipitation biases over this region. By using convection-permitting multi-season simulations over the contiguous United States coupled with an operational-like irrigation scheme, we show that irrigation increases surface evapotranspiration and decreases surface temperature by increasing evaporative fraction. By increasing the frequency of mesoscale convective systems, irrigation reduces the summertime model precipitation deficit and improves the simulated precipitation diurnal cycle over the Great Plains. The increased precipitation also alleviates the warm bias in our simulation setup, likely by damping the positive feedback between soil moisture and temperature.
Profiles of overall survival-related gene expression-based risk signature and their prognostic implications in clear cell renal cell carcinoma
Authors: He Zihao , Deng Tuo , Duan Xiaolu , Zeng Guohua 
Published: August 2020

Abstract
The present work aimed to evaluate the prognostic value of overall survival (OS)-related genes in clear cell renal cell carcinoma (ccRCC) and to develop a nomogram for clinical use. Transcriptome data from The Cancer Genome Atlas (TCGA) were collected to screen differentially expressed genes (DEGs) between ccRCC patients with OS &gt; 5 years (149 patients) and those with &lt;1 year (52 patients). In TCGA training set (265 patients), seven DEGs (cytochrome P450 family 3 subfamily A member 7 (CYP3A7), contactin-associated protein family member 5 (CNTNAP5), adenylate cyclase 2 (ADCY2), TOX high mobility group box family member 3 (TOX3), plasminogen (PLG), enamelin (ENAM), and collagen type VII α 1 chain (COL7A1)) were further selected to build a prognostic risk signature by the least absolute shrinkage and selection operator (LASSO) Cox regression model. Survival analysis confirmed that the OS in the high-risk group was dramatically shorter than their low-risk counterparts. Next, univariate and multivariate Cox regression revealed the seven genes-based risk score, age, and Tumor, lymph Node, and Metastasis staging system (TNM) stage were independent prognostic factors to OS, based on which a novel nomogram was constructed and validated in both TCGA validation set (265 patients) and the International Cancer Genome Consortium cohort (ICGC, 84 patients). A decent predictive performance of the nomogram was observed, the C-indices and corresponding 95% confidence intervals of TCGA training set, validation set, and ICGC cohort were 0.78 (0.74–0.82), 0.75 (0.70–0.80), and 0.70 (0.60–0.80), respectively. Moreover, the calibration plots of 3- and 5 years survival probability indicated favorable curve-fitting performance in the above three groups. In conclusion, the proposed seven genes signature-based nomogram is a promising and robust tool for predicting the OS of ccRCC, which may help tailor individualized therapeutic strategies.
The effect of copy number on α-synuclein’s toxicity and its protective role in Bax-induced apoptosis, in yeast
Authors: Akintade Damilare D. , Chaudhuri Bhabatosh 
Published: August 2020

Abstract
Apoptosis is a form of programmed cell death which is essential for the growth of dividing human cells whereas, in contrast, it is deleterious for post-mitotic cells such as neurons. Bax and α-synuclein are two human proteins which play a role in the induction of neuronal apoptosis in neurodegenerative diseases like Alzheimer’s and Parkinson’s. Human Bax and α-synuclein also induce cell death when expressed in baker’s yeast, Saccharomyces cerevisiae. Quite unexpectedly, the human α-synuclein gene had been identified as an inhibitor of pro-apoptotic Bax using a yeast-based screen of a human hippocampal cDNA library. Plasmids were constructed with different promoters, which allow expression of wildtype and Parkinson’s disease (PD)-related mutant α-synuclein genes, from (i) multi-copy 2µ (episomal) plasmids and (ii) integrative plasmids that compel expression of genes from chromosomal sites in varying copy numbers (1–3). All α-synuclein-containing plasmids were introduced, through transformation, into a yeast strain which already contained a chromosomally integrated copy of Bax. It is for the first time that it was observed that, depending on gene dosage, only wildtype α-synuclein is anti-apoptotic while mutant α-synuclein is not. The results also indicate that wildtype α-synuclein has a remarkable ability to manifest two contrasting effects depending on its level of expression: (i) normally, it would negate apoptosis but (ii) when overexpressed, it tends to induce apoptosis which is probably what happens in PD.
Transplantation of platelet-derived mitochondria alleviates cognitive impairment and mitochondrial dysfunction in db/db mice
Authors: Ma Hongli , Jiang Tao , Tang Wenxin , Ma Zhi , Pu Kairui , Xu Fuxing , Chang Haiqing , Zhao Ge , Gao Wei , Li Yansong , Wang Qiang 
Published: August 2020

AbstractDiabetes-associated cognitive impairment (DACI) can increase the risk of major cardiovascular events and death. Neuronal functionality is highly dependent on mitochondria and emerging evidence has shown that mitochondrial transplantation is a potential and effective strategy that can reduce brain injury and associated disorders. Platelets are abundant in blood and can be considered a readily available source of small-size mitochondria. These cells can be easily acquired from the peripheral blood with minimal invasion via simple venipuncture. The present study aimed to investigate whether transplantation of platelet-derived mitochondria (Mito-Plt) could improve DACI. Cognitive behaviors were assessed using the Morris water maze test in db/db mice. The results demonstrated that Mito-Plt was internalized into hippocampal neurons 24 h following intracerebroventricular injection. Importantly, one month following Mito-Plt transplantation, DACI was alleviated in db/db mice and the effect was accompanied with increased mitochondrial number, restored mitochondrial function, attenuated oxidative stress and neuronal apoptosis, as well as decreased accumulation of Aβ and Tau in the hippocampus. Taken together, the data demonstrated that transplantation of Mito-Plt attenuated cognitive impairment and mitochondrial dysfunction in db/db mice. This method may be a potential therapeutic application for the treatment of DACI.