bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic Value of Various Nutritional Assessment Indicators on Long Term and Short Term Outcomes for Patients with High Grade Osteosarcoma Receiving Surgical Resection
Authors: Yang Yuhan , Xuelei Ma , Ding Xinyan 
Published: August 2020

Abstract
Background: Many researchers have focused on exploring the association between patients’ nutritional status and clinical outcomes with some easy-to-reach indicators, especially in some carcinoma with high incidence. However, there was little attention on sarcomas and the objective of this study was to evaluate the prognostic value of some innovative nutrition associated indexes on patients with high grade osteosarcoma receiving surgical excision. Method: We retrospectively included patients’ clinical characteristics diagnosed as high grade osteosarcomas histologically receiving surgical excision from January 2008 to June 2018. Body mass index (BMI), Glasgow prognostic score (GPS), systematic inflammatory index (SII), and controlling nutritional (CONUT) score were calculated as nutritional associated factors to evaluate their prognostic value. The primary outcome was overall survival (OS) while the secondary outcome was the postoperative length of hospitalization. The relationship between clinical features and outcomes were performed by Cox and logistic regression analysis, respectively. The independent prognostic factors were chosen to construct predicted model whose internal and external accuracy were validated by concordance index (C-index), Brier score, and calibration plots.Results: High score of GPS predicted worse OS [HR (95% CI): 3.122 (1.982-4.918) versus 2.208 (1.014-4.804)] and higher rank of CONUT predicted poorer prognosis [HR (95% CI): 2.573 (1.616-4.097)] independently. The CONUT score was selected as the only prognostic factor on the length of hospitalization [HR (95% CI): 2.137 (1.270-3.596)]. The nomogram plots were used to visualize the results of predicted models whose performance was evaluated from the aspects of calibration and discrimination. Conclusion: Our study suggested prognostic value of nutritional assessment indexes including GPS and CONUT score that appropriate preoperative intervention which could optimize patients’ nutrition associated indicators may improve prognosis on patients with high grade osteosarcoma receiving surgical excision.Level of evidence: Level Ⅲ, prognostic study
Will Virtual Multidisciplinary Team Meetings Become the Norm for Musculoskeletal Oncology Care following the COVID-19 Pandemic? - Experience from a tertiary sarcoma centre
Authors: Rajasekaran Raja Bhaskara , Whitwell Duncan , Cosker Thomas D.A. , Gibbons Christopher L.M.H , Carr Andrew 
Published: August 2020

Abstract
Background: Like with all cancers, multidisciplinary team (MDT) meetings are the norm in bone tumour and soft tissue sarcoma (BST) management too. Problem in attendance of specialists due to geographical location is the one of the key barriers to effective functioning of MDTs. To overcome this problem, virtual MDTs involving videoconferencing or telemedicine have been proposed, but however this has been seldom used and tested. The COVID-19 pandemic forced the implementation of virtual MDTs in the Oxford sarcoma service in order to maintain normal service provision. We conducted a survey among the participants to evaluate its efficacy.Methods: An online questionnaire comprising of 24 questions organised into 4 sections was circulated among all participants of the MDT after completion of 8 virtual MDTs. Opinions were sought comparing virtual MDTs to the conventional face-to-face MDTs on various aspects. A total of 36 responses were received and were evaluated.Results: 72.8% were satisfied with the depth of discussion in virtual MDTs and 83.3% felt that the decision making in diagnosis had not changed following the switch from face-to-face MDTs. About 86% reported to have all essential patient data was available to make decisions and 88.9% were satisfied with the time for discussion of patient issues over virtual platform. Three-fourths of the participants were satisfied (36.1% - highly satisfied; 38.9% - moderately satisfied) with virtual MDTs and 55.6% of them were happy to attend MDTs only by the virtual platform in future. Regarding future, 77.8% of the participants opined that virtual MDTs would be the future of cancer care and an overwhelming majority (91.7%) felt that the present exercise would serve as a precursor to global MDTs involving specialists from abroad in future.Conclusion: Our study shows that the forced switch to virtual MDTs in sarcoma care following the unprecedented COVID-19 pandemic to be a viable and effective alternative to conventional face-to-face MDTs. With effective and efficient software in place, virtual MDTs would also facilitate in forming extended MDTs in seeking opinions on complex cases from specialists abroad and can expand cancer care globally.
Serotyping of sub-Saharan Africa Salmonella Strains Isolated from Different Sources Using Multiplex PCR and Capillary Electrophoresis Analysis and whole Genome Sequencing
Authors: Kagambèga Assèta , Hiott Lari M. , Boyle David S. , McMillan Elizabeth A. , Haukka Kaisa , Sharma Poonam , Gupta Sushim K. , Ramadan Hazem , Cho Sohyun , Humayoun Shaheen B. , Woodley Tiffanie A. , Barro Nicolas , Jackson Charlene R. , Frye Jonathan G. 
Published: August 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
LncRNA EPIC1 Promotes Cancer Cell Proliferation and Inhibits Apoptosis in Gallbladder Cancer Interacting with lncRNA LET
Authors: Fu Changbo , Nie Lei , Yin Tao , Xu Xuan , lu weijun 
Published: August 2020

Abstract
Background: LncRNA EPIC1 is likely involved in human cancer by promoting cell cycle progression. Our study was carried out to investigate the involvement of EPIC1 in gallbladder cancer (GBC). Methods: Expression levels of EPIC1 in two types of tissues (GBC and paracancerous) and plasma were measured by performing qPCR. GBC-SD and SGC-996 cells were transfected with LET and EPIC1 expression vectors.Results: In the preset study we found that EPIC1 was upregulated in tumor tissues than in paracancerous tissues of GBC patients, and plasma levels of EPIC1 were significantly correlated with levels of EPIC1 in tumor tissues. LncRNA LET was downregulated in tumor tissues than in paracancerous tissues and was inversely correlated with EPIC1 in both tumor tissues and paracancerous tissues. Overexpression of EPIC1 led to downregulated LET, and LET overexpression also mediated the downregulation of EPIC1. EPIC1 led to accelerated GBC cell proliferation and inhibited apoptosis. Overexpression of LET played opposites roles. In addition, overexpression of LET also attenuated the effects of EPIC1 overexpression on cancer cell proliferation and apoptosis. Conclusion: Therefore, therefore, lncRNA EPIC1 may promote cancer cell proliferation and inhibit apoptosis in GBC by interacting with LET.
Comparable Safety of ERCP in Symptomatic and Asymptomatic Patients with Common Bile Duct Stones: A Propensity-Matched Analysis
Authors: Xiao Lina , Geng Chong , Li Xiao , Li Yanni , Wang Chunhui 
Published: August 2020

Abstract
Background: The safety of endoscopic retrograde cholangiopancreatography (ERCP) for asymptomatic common bile duct (CBD) stones patients has not been fully studied. This study aimed to compare the incidence and severity of ERCP complications in patients with asymptomatic and symptomatic CBD stones and to provide evidence for the treatment of asymptomatic CBD stones.Methods: The clinical data of patients were retrospectively analyzed. These patients were divided into the asymptomatic CBD stones group and the symptomatic CBD stones group. Propensity score matching (PSM) was used to match the two groups. The incidence and severity of postoperative complications of ERCP in the two groups were analyzed.Results: A total of 79 patients who had asymptomatic CBD stones and 795 patients who had symptomatic CBD stones were included in this study. After PSM, 79 patients from asymptomatic CBD group and 316 patients from the symptomatic CBD stones group were identified. Before and after PSM, there were no significant differences in the incidence and severity of post-ERCP pancreatitis (PEP) between the two groups (P> 0.05). Besides, the incidence and severity of other complications including acute cholangitis, bleeding and perforation in the two groups was no differences before and after PSM (P>0.05).Conclusions: Patients with asymptomatic CBD stones are not at higher risk of getting ERCP-related complications when compared with those with symptomatic ones. ERCP is as safe and effective for asymptomatic CBD stones as for symptomatic patients.
Dynamic Space Use of Andalusian Rice Fields by Overwintering Lesser Black-Backed Gulls is Driven by Harvest-Related Flooding
Authors: Rees Charles B. van , Aragonés David , Bouten Willem , Thaxter Chris B. , Stienen Eric W.M. , Bustamante Javier , Green Andy 
Published: August 2020

Abstract
Background: Research on the space use and behavior of waterbirds—as highly mobile of wetland habitats—yields important insights on human-wildlife interactions of ecological and societal importance under global change. The extent to which dynamic (within-season) changes in anthropogenic landscapes affects these interactions is poorly understood. Lesser black-backed gulls (Larus fuscus) are prominent biovectors of biological and artificial materials, and have exhibited large population increases in parts of southern Europe in recent decades. Methods: We combined GPS tracking, earth observation, accelerometry and field observations to study the space use of overwintering in a mixed rice field landscape in Andalusia, Southern Spain. We used Manly selectivity metrics and classified remote sensing imagery to directly evaluate space use and habitat selection for these gulls and how it changed throughout the rice harvest cycle. Results: Analysis of over 45,000 GPS fixes and 14 classified remotely-sensed images from winter 2016-17 showed dynamic space use driven by the harvest-related flooding across the rice harvest cycle. Prior to harvest, gulls foraged in rice paddies during the day and roosted in adjacent waterbodies (the River Guadalquivir, and fish ponds) at night. During harvest, they spent nearly 100% of their daily cycle within the rice fields, foraging in harvested paddies and roosting in post-harvest, flooded paddies. After harvest, they roosted in flooded fields at night and foraged at landfills in the surrounding landscape. Conclusion: Gull space use at landscape and paddy scales was closely linked to dynamic land and water management over the rice agricultural cycle, illustrating the detailed scales at which human activities influence the movements of ecologically important, human-associated biovectors like gulls. The frequent early-spring movement patterns of these increasingly numerous gulls between landfills and agricultural fields an important biotransport link for potentially toxic materials between waste sites and places where food is grown for human consumption.
Sedation Practices for Routine Gastrointestinal Endoscopy: A Systematic Review of Recommendations
Authors: Dossa Fahima , Megetto Olivia , Yakubu Mafo , Zhang David D.Q. , Baxter Nancy N. 
Published: August 2020

Abstract
Background: Sedation is commonly used in gastrointestinal endoscopy; however, considerable variability in sedation practices has been reported. The objective of this review was to identify and synthesize existing recommendations on sedation practices for routine gastrointestinal endoscopy procedures.Methods: We systematically reviewed guidelines and position statements identified through a search of PubMed, guidelines databases, and websites of relevant professional associations from January 1, 2005 to May 10, 2019. We included English-language guidelines/position statements with recommendations relating to sedation for adults undergoing routine gastrointestinal endoscopy. Documents with guidance only for complex endoscopic procedures were excluded.We extracted and synthesized recommendations relating to: 1) choice of sedatives, 2) sedation administration, 3) personnel responsible for monitoring sedated patients, 4) skills and training of individuals involved in sedation, and 5) equipment required for monitoring sedated patients. We assessed the quality of included documents using the Appraisal of Guidelines for Research & Evaluation (AGREE) II tool.Results: We identified 19 guidelines and 7 position statements meeting inclusion criteria. Documents generally agreed that a single, trained registered nurse can administer moderate sedation, monitor the patient, and assist with brief, interruptible tasks. Documents also agreed on the routine use of pulse oximetry and blood pressure monitoring during endoscopy. However, recommendations relating to the drugs to be used for sedation, the healthcare personnel capable of administering propofol and monitoring patients sedated with propofol, and the need for capnography when monitoring sedated patients varied. Only 9 documents provided a grade or level of evidence in support of their recommendations.Conclusions: Recommendations for sedation practices in routine gastrointestinal endoscopy differ across guidelines/position statements and often lack supporting evidence with potential implications for patient safety and procedural efficiency.Registration Number: CRD42019141076
Validity and Reliability of Smartphone Applications for Measurement of Hip Rotation, Compared With Three-dimensional Motion Analysis
Authors: Ganokroj Phob , Sompornpanich Nuchanun , Kerdsomnuek Pichitpol , Vanadurongwan Bavornrat , Lertwanich Pisit 
Published: August 2020

Abstract
BackgroundMeasurement of hip rotation is a crucial clinical parameter for the identification of hip problems and the monitoring of symptoms. The objective of this study was to determine whether the use of two smartphone applications is valid and reliable for the measurement of hip rotation.MethodsAn experimental, cross-sectional study was undertaken to assess passive hip internal and external rotation in three positions by two examiners. The hip rotational angles were measured by a smartphone clinometer application in the sitting and prone positions, and by a smartphone compass application in the supine position; their results were compared with those of the standard, three-dimensional, motion analysis system. The validities and inter-rater and intra-rater reliabilities of the smartphone applications were evaluated.ResultsThe study involved 24 participants. The validities were good to excellent for the internal rotation angles in all positions (ICC 0.81–0.94), good for the external rotation angles in the prone position (ICC 0.79), and fair for the sitting and supine positions (ICC 0.70–0.73). The measurement of the hip internal rotation in the supine position had the highest ICC value of 0.94 (0.91, 0.96). The two smartphone applications showed good-to-excellent intra-rater reliability, but good-to-excellent inter-rater reliability for only three of the six positions (two other positions had fair reliability, while one position demonstrated poor reliability).ConclusionsThe two smartphone applications have good-to-excellent validity and intra-rater reliability, but only fair-to-good inter-rater reliability for the measurement of the hip rotational angle.Trial registration: Number20181022003 at the Thai Clinical Trials Registry (http://www.clinicaltrials.in.th) which was retrospectively registered at 2018-10-18 15:30:29.http://www.clinicaltrials.in.th/index.php?tp=utf8&task=regtrial&id=4100&menu=regtrial&smenu=trialmodify&ts=20200711091413
Differing Perspectives of Learning and Teaching in Culturally Diverse Postgraduate Classrooms: A Qualitative Descriptive Study.
Authors: markey kathleen , Graham Margaret M , Tuohy Dympna , McCarthy Jane , O’Donnell Claire , Hennessy Therese , Fahy Anne , Brien Brid O’ 
Published: August 2020

Abstract
Background:Higher Education Institutes are experiencing a widening cultural diversity of student populations and campus communities. With expanding globalisation, growing international education initiatives and increasing incoming international postgraduate student numbers, the cultural diversity of the classroom will continue to magnify. The expanding diversity of the student population provides a fertile environment for intercultural learning and can support the development of intercultural competence if structured and facilitated appropriately. However, meeting the individual and collective needs of postgraduate students in a widening culturally diverse learning environment is not without its challenges. A greater understanding of factors that enhance and constrain quality and inclusive learning in culturally diverse classrooms is required as a means of identifying structures, supports and educational approaches needed. The purpose of this study is to describe the perceptions and experiences of students (home and international students) and faculty of learning within a culturally diverse postgraduate programme, delivered through blended learning approaches. Methods: This qualitative descriptive study explored and triangulated data from three data sets. This approach guided the exploration of experiences of faculty and students (both home and international students) in capturing detailed descriptions of perspectives and experiences of learning in culturally diverse environments.Results: Three themes were generated from the data, namely representing students (both home and international students) and faculty participants’ perspectives of learning in culturally diverse environments: early apprehension, cautious engagement and shared acceptance. Conclusions: This study highlights the multifaceted responses of teaching faculty and students to cultural diversity in the learning environment, adding new dimensions to the existing discourse on intercultural learning and development. It illuminates the individuality of participants' learning experiences and draws attention to the complexities associated with meeting individual and collective learning needs of postgraduate students who come from diverse cultural, linguistic, educational and clinical experience backgrounds. However, this study illuminates how perceptions of cultural difference can also impact on learning behaviours and cohesive learning. Creating positive intercultural inclusiveness, culturally responsive teaching and nurturing capacity to see differing perspectives, oneself and others in more inclusive ways is essential in supporting quality postgraduate student learning.
Genome-Wide Identification of MicroRNAs Associated With the Somatic Embryogenesis in Eucalyptus
Authors: Qin Zihai , Li Junji , Zhang Ye , Xiao Yufei , Zhang Xiaoning , Liu Hailong , Chen Bowen 
Published: August 2020

Abstract
Background: MicroRNAs (miRNAs) are a class of small noncoding RNAs with 18-24 nucleotides in length and function in many biological processes in plant. Although Eucalyptus trees are widely planted across the world, our understanding of the miRNA regulation in the somatic embryogenesis of Eucalyptus is still poor. Here we reported for the first time the miRNA profiles of differentiated and dedifferentiated tissues of two Eucalyptus cultivars and identified miRNAs involved in the somatic embryogenesis of Eucalyptus.Results: Stem and tissue-culture induced callus were obtained from the subculture seedlings of E. camaldulensis and E. grandis x urophylla, and were used as differentiated and dedifferentiated samples, respectively. We generated 346.4 million reads for 12 samples (n=3) and identified 888 miRNA precursors (197 known and 691 novel) which can produce 1,067 mature miRNAs. These miRNAs were mainly distributed in chromosomes Chr03, Chr05 and Chr08, and can produce 46 miRNA clusters. In these samples we detected 998 miRNAs with TPM (transcripts per million reads) > 5 and found that highly expressed miRNAs varied across samples. We identified 327 and 343 differentially expressed miRNAs in the dedifferentiation process of E. camaldulensis and E. grandis x urophylla, respectively. Dysregulated miRNAs shared by the two cultivars might be involved in the development of embryonic callus of Eucalyptus, such as MIR156, MIR159, MIR160, MIR164, MIR166, MIR169, MIR171, MIR399 and MIR482. We also identified 81 up-regulated (e.g., miR159c-3p, miR167a-5p, miR397a-3p, miR397c-5p, miR397d-3p, miR397d-5p, N-miR1-5p and N-miR5-5p) and 67 down-regulated (e.g., miR482b-3p, N-miR3-3p, miR156a-3p, N-miR40-3p and N-miR18-5p) miRNAs specific to E. camaldulensis. Target prediction and functional analysis showed they might be involved in longevity regulating and plant hormone signal transduction pathways. Then, the expression patterns of these miRNAs were confirmed by qRT-PCR. Conclusions: This is the first time to study the miRNAs profiles in the dedifferentiation process of Eucalyptus and it will provide a valuable resource for future studies. More importantly, our findings will improve our understanding of miRNA regulation and molecular mechanisms during the somatic embryogenesis of Eucalyptus, and the output of this study will benefit the Eucalyptus breeding program.
A Nursing Innovation Workshop to Improve Clinical Nurses' Innovation Ability: a Quasi-experimental Study
Authors: Gao Limei , Lu Qiaocong , Ou Jiefen , Hou Xiangchuan , Wang Mingzhu 
Published: August 2020

Abstract
Background: Currently, the innovation ability of nurses in China is at a medium to low level. Most studies focus on nurses' innovation ability and its influencing factors, while few interventional studies consider how to improve nurses' innovation ability. The aim of the study is to explore the effects of nursing innovation workshops in improving clinical nurses' innovation ability.Methods: This Quasi-experimental study was conducted in a tertiary hospital of Guangdong. Thirty-seven recruited nurses participated in a nursing innovation workshop for half a year. Questionnaire data were collected on nurses’ self-rated innovation ability and research ability before the training and 3 months after finishing the training.Results: Nurses’ self-rated innovation ability and research ability improved significantly after the training (P < 0.001).Conclusions: A nursing innovation workshop is an effective and feasible teaching method that can promote the generation of innovative thinking and improve the innovative ability of nurses.