bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphorylation influences water and ion channel function of AtPIP2;1
Authors: Qiu Jiaen , McGaughey Samantha A. , Groszmann Michael , Tyerman Stephen D. , Byrt Caitlin S. 
Published: July 2020

AbstractThe phosphorylation state of two serine residues within the C‐terminal domain of AtPIP2;1 (S280, S283) regulates its plasma membrane localization in response to salt and osmotic stress. Here, we investigated whether the phosphorylation state of S280 and S283 also influence AtPIP2;1 facilitated water and cation transport. A series of single and double S280 and S283 phosphomimic and phosphonull AtPIP2;1 mutants were tested in heterologous systems. In Xenopus laevis oocytes, phosphomimic mutants AtPIP2;1 S280D, S283D, and S280D/S283D had significantly greater ion conductance for Na+ and K+, whereas the S280A single phosphonull mutant had greater water permeability. We observed a phosphorylation‐dependent inverse relationship between AtPIP2;1 water and ion transport with a 10‐fold change in both. The results revealed that phosphorylation of S280 and S283 influences the preferential facilitation of ion or water transport by AtPIP2;1. The results also hint that other regulatory sites play roles that are yet to be elucidated. Expression of the AtPIP2;1 phosphorylation mutants in Saccharomyces cerevisiae confirmed that phosphorylation influences plasma membrane localization, and revealed higher Na+ accumulation for S280A and S283D mutants. Collectively, the results show that phosphorylation in the C‐terminal domain of AtPIP2;1 influences its subcellular localization and cation transport capacity.
Divisions of regulatory labor, institutional closure, and structural secrecy in new regulatory states: The case of neglected liquidity risks in market‐based banking
Authors: Wansleben Leon 
Published: July 2020

AbstractMonetary policy and financial regulation have been regarded one of the paradigmatic domains where states can reap the benefits of delegating clearly delineated and unequivocal policy tasks to specialized technocrats. This article discusses the negative side‐effects and unintended consequences of particular ways to partition such tasks. The separation between monetary policy formulation, central banks' money market management, and financial supervision has weakened policy makers' capacities to address risks associated with banks' active liability management and to mitigate pro‐cyclical dynamics in money markets. These adverse effects derive from the neglect of liquidity as a regulatory problem in formal task descriptions; from the obstacles to positive coordination between specialized technocrats in the self‐contained domains of monetary policy formulation, implementation, and prudential regulation; and from a dissociation between formal responsibilities, which lie with regulators, and the resources residing in money market divisions – market expertise and influence over counterparties – that are critical to influence bank behavior. I explore these mechanisms with an in‐depth case study of British monetary and financial governance between 1970 and 2007. I posit that we can generalize the respective mechanisms to explore unintended effects of ‘agencification’ and to contribute to a broader re‐assessment of those organizing principles that have guided the construction of ‘new regulatory states’.
Robust estimation for discrete‐time state space models
Authors: Aeberhard William H. , Cantoni Eva , Field Chris , Künsch Hans R. , Mills Flemming Joanna , Xu Ximing 
Published: July 2020

AbstractState space models (SSMs) are now ubiquitous in many fields and increasingly complicated with observed and unobserved variables often interacting in nonlinear fashions. The crucial task of validating model assumptions thus becomes difficult, particularly since some assumptions are formulated about unobserved states and thus cannot be checked with data. Motivated by the complex SSMs used for the assessment of fish stocks, we introduce a robust estimation method for SSMs. We prove the Fisher consistency of our estimator and propose an implementation based on automatic differentiation and the Laplace approximation of integrals which yields fast computations. Simulation studies demonstrate that our robust procedure performs well both with and without deviations from model assumptions. Applying it to the stock assessment model for pollock in the North Sea highlights the ability of our procedure to identify years with atypical observations.
Business cycle duration dependence and foreign recessions
Authors: de Bondt Gabe , Vermeulen Philip 
Published: July 2020

AbstractThis study revisits business cycle duration dependence in G7 countries by controlling for foreign recessions, defined as the number of other G7 countries in a recession. Estimates from regime switching logit models show that the monthly likelihood of ending an expansion roughly doubles for every extra G7 country in recession, but the end of foreign recessions do not affect the ending of recessions. They also show that recessions are duration dependent in all G7 countries, but expansions only in the United States and Germany. The economic importance of foreign recessions and duration in driving business cycle phase changes vary across countries.
Suppurative otitis media in Angola: clinical and demographic features
Authors: Filipe Matuba , Karppinen Mariia , Kuatoko Palmira , Reimer Åke , Riesbeck Kristian , Pelkonen Tuula 
Published: July 2020

AbstractObjectiveTo describe the demographics and clinical findings in patients with otorrhoea in Angola.MethodsA total of 411 patients with otorrhoea presenting in the ear, nose and throat clinic in Luanda and healthcare centres in other Angolan provinces underwent interview and clinical examination. We describe the demographics and clinical characteristics of the patients.ResultsThe majority (64%) of patients were children <15 years (age ranged from 1 month to 77 years; median age 10.9 years) while 31% were children <5 years. In 83% of the patients, otorrhoea had lasted >14 days at the time of the examination indicating chronic suppurative otitis media (CSOM), which was confirmed with otoscopy in 72% of patients. Acute otitis media occurred in 16% of patients and was more common in children than in adults (22% vs. 10%; P = 0.007). Median duration of otorrhoea was >12 months. Earache (67%), fever (20%), dizziness (17%), nausea and/or vomiting (6%) were the main symptoms. Adult patients reported noticing hearing impairment (HI) more often than the parents of child patients (72% vs. 50%; P < 0.0001). Reported HI correlated with otorrhoea duration (P < 0.0001), presence of earache, dizziness, and measles or meningitis in history. The level of education in the family did not correlate with symptom duration.ConclusionsOtorrhoea is mainly due to CSOM and affects patients long‐term in Angola. Otorrhoea duration is the strongest predictor of HI. Education on OM and its treatment is needed to prevent HI.
Iron overload is related to elevated blood glucose levels in obese children and aggravates high glucose-induced endothelial cell dysfunction in vitro
Authors: Wu Wei , Yuan Jinna , Shen Yu , Yu Yunxian , Chen Xuefeng , Zhang Li , Huang Ke , Zhan Jianying , Dong Guan-Ping , Fu Junfen 
Published: July 2020

IntroductionThis study was performed to investigate the role of iron overload in the early stage of hyperglycemia-induced vascular functional impairment.Research design and methodsA total of 196 obese children were enrolled, and data regarding ferritin levels, blood glucose levels, intima-media thickness of carotid arteries, liver function and fibrosis index, hemoglobin, blood pressure, blood lipids, and inflammation indicators were collected. Ferritin levels were compared with a control group, which consisted of 148 healthy non-obese children who were age-matched and gender-matched. Endothelial cells were cultured in high glucose medium and supplemented with ferric citrate with or without iron remover (deferoxamine), a reducing agent (N-acetyl-cysteine), or a nuclear factor-κB (NF-κB) inhibitor (BAY 11-7082). Apoptosis, oxidative stress, nitric oxide levels, and endothelin content were evaluated. DNA microarray analysis was performed to analyze the expression of genes in the NF-κB signaling pathway.ResultsObese children have significantly higher ferritin levels compared with the control group. Ferritin level was positively correlated with hemoglobin and was related to metabolic disorders, including impaired glucose tolerance, higher blood pressure, dyslipidemia, and impaired hepatic function. Endothelial cells treated with ferric citrate showed a significantly higher rate of apoptosis, higher levels of oxidative stress, and impaired vasomotor function under high glucose conditions. The above effects were rescued by treatment with an iron remover, reducing agent, or NF-κB inhibitor. Further, detection of phosphorylated-p65 distribution in cells confirmed activation of the NF-κB pathway. DNA microarrays and subsequent gene oncology enrichment analyses revealed the main processes activated in cells.ConclusionIncreased ferritin levels are related to impaired glucose tolerance and other metabolic disorders in obese children. At the cellular level, iron overload aggravated the endothelial cell dysfunction caused by high glucose.
Improved outcomes in cystic fibrosis using modified Re-Education of Airway Clearance Technique (REACT) programme
Authors: Reamer Courtney , O'Malley Catherine , Nufer Julie , Savant Adrienne 
Published: July 2020

Background and objectivesCystic fibrosis (CF) is known to reduce lung function as measured by per cent predicted for the forced expiratory volume in the first second (ppFEV1) over time. Our paediatric CF programme demonstrated significant gaps in benchmarked ppFEV1predicted compared with the national median. Our objective was to assess whether the implementation of a modifiedRe-Education ofAirwayClearanceTechniques (REACT) programme could lead to an improvement in lung function as measured by ppFEV1.MethodsThis 2-year prospective quality improvement study at Lurie Children’s CF Center for children aged >6 years used improvement methodology to implement a modified REACT programme. Outcome measures were assessed for our entire programme via the CF Foundation Patient Registry (CFFPR) and statistical process control. Comparisons were also made before and after REACT for outcome measures.ResultsBy the end of implementation, monthly participation rate achieved 100%. Using CFFPR data and SPC, median ppFEV1increased by 3.9%, whereas only body mass index (BMI) as a secondary outcome increased. Comparison of pre and post REACT showed improvements in average ppFEV1(95% vs 96%, p<0.0001), FEF25%−75%(82% vs 83%, p=0.0590), rate of ppFEV1 decline (+2% vs −4%, p=0.0262) and BMI percentile (57% vs 60%, p<0.0001).ConclusionsImplementation of a modified REACT at Lurie Children’s paediatric CF programme led to an increase in ppFEV1, FEF25%−75%and BMI percentile.
Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells
Authors: Iida Yuichi , Yoshikawa Rintaro , Murata Akihiko , Kotani Hitoshi , Kazuki Yasuhiro , Oshimura Mitsuo , Matsuzaki Yumi , Harada Mamoru 
Published: July 2020

BackgroundMesenchymal stem/stromal cells (MSC) accumulate and reside in tumor sites.MethodsTaking advantage of this feature in anticancer therapy, immortalized murine MSC (iMSC) were genetically altered to produce chemokine (C-C motif) ligand 19 (iMSC/CCL19), which attracts dendritic cells (DC) and T lymphocytes. Thereafter, iMSC/CCL19 were examined for their therapeutic efficacy using a syngeneic CT26 colon carcinoma cell line.ResultsCo-injection of iMSC/CCL19 into mice significantly suppressed the in vivo growth of CT26 cells compared with that of CCL19-expressing immortalized fibroblasts (iFib/CCL19). This anticancer effect was not observed when injected in CT26-bearing nude mice. Co-injected iMSC/CCL19 survived longer than iFib/CCL19 in the tumor sites. In a therapeutic model, local injection of iMSC/CCL19 suppressed the tumor growth, and increased IFN (interferon)-γ+CD8+T cells and CCR7+DC infiltration in tumor site was observed when treated with iMSC/CCL19, but not with iMSC. This antitumor effect was completely negated by depletion of CD4+cells and partially negated by depletion of CD8+cells. Furthermore, the antitumor effects induced by local injection of iMSC/CCL19 were augmented by additional therapy with anti-programmed death (PD)-ligand 1 (PD-L1) antibody, but not with anti-PD-1 antibody. This combination therapy cured most of the tumors in CT26-bearing mice.ConclusionThese results suggest that local therapy with iMSC/CCL19 can suppress tumor growth via effective recruitment of CCR7+DC into tumor sites and increase IFN-γ+CD8+T cells, and that combination with anti-PD-L1 antibody therapy can be a powerful anticancer therapy.
Jeans instability in non-minimal matter-curvature coupling gravity
Authors: Gomes Cláudio 
Published: July 2020

AbstractThe weak field limit of the nonminimally coupled Boltzmann equation is studied, and relations between the invariant Bardeen scalar potentials are derived. The Jean’s criterion for instabilities is found through the modified dispersion relation. Special cases are scrutinised and considerations on the model parameters are discussed for Bok globules.
Reliability Analysis of the Generalized Exchanged Hypercube
Authors: Zhang Qifan , Xu Liqiong , Yang Weihua , Yin Shanshan 
Published: July 2020

Let [Formula: see text] be a non-complete graph, a subset [Formula: see text] is called a [Formula: see text]-component cut of [Formula: see text], if [Formula: see text] is disconnected and has at least [Formula: see text] components. The cardinality of the minimum [Formula: see text]-component cut is the [Formula: see text]-component connectivity of [Formula: see text] and is denoted by [Formula: see text]. The [Formula: see text]-component connectivity is a natural extension of the classical connectivity. As an application, the [Formula: see text]-component connectivity can be used to evaluate the reliability and fault tolerance of an interconnection network structure based on a graph model. In a previous work, E. Cheng et al. obtained the [Formula: see text]-component connectivity of the generalized exchanged hypercube [Formula: see text] for [Formula: see text] and [Formula: see text]. In this paper, we continue the work and determine that [Formula: see text] for [Formula: see text]. Moreover, we show that every optimal [Formula: see text]-component cut of [Formula: see text] is trivial for [Formula: see text] and [Formula: see text].
Searching for diboson resonances in the boosted all-hadronic final state at s = 13 TeV with CMS
Authors: Aarrestad Thea 
Published: July 2020

This article summarizes three searches for diboson resonances in the all-hadronic final state using data collected at a center-of-mass energy of [Formula: see text] = 13 TeV with the CMS experiment at the CERN LHC. The boson decay products are contained in one large-radius jet, resulting in dijet final states which are resolved using jet substructure techniques. The analyses presented use 2.3 fb[Formula: see text], 35.9 fb[Formula: see text] and 77.3 fb[Formula: see text] of data collected between 2015 and 2017. These include the first search for diboson resonances in data collected at a 13 TeV collision energy, the introduction of a new algorithm to tag vector bosons in the context of analyzing the data collected in 2016, and the development of a novel multidimensional fit improving on the sensitivity of the previous search method with up to 30%. The results presented here are the most sensitive to date of all diboson resonance searches in the dijet final state. An emphasis on improvements in technique for vector boson tagging is made.
A new pressure-parametric cosmological model
Authors: Yao Yan-Hong , Meng Xin-He 
Published: July 2020

We put forward a pressure-parametric model to study the tiny deviation from cosmological constant(CC) behavior of the dark sector accelerating the expansion of the Universe. Data from cosmic microwave background (CMB) anisotropies, baryonic acoustic oscillations (BAO), Type Ia supernovae (SN Ia) observation are applied to constrict the model parameters. The constraint results show that such model suffers with [Formula: see text] tension as well. To realize this model more physically, we reconstruct it with the quintessence and phantom scalar fields, and find out that although the model predicts a quintessence-induced acceleration of the Universe at past and present, at some moment of the future, dark energy’s density have a disposition to increase.
Probing kinematics and fate of Bianchi type V Universe
Authors: Goswami G. K. , Yadav Anil Kumar , Mishra B. 
Published: July 2020

In this paper, we have constrained the cosmological parameters of Bianchi type V universe by bounding the model under consideration with recent observational [Formula: see text] and Pantheon data. It is assumed that the energy conservation law holds separately for barotropic fluid and dark energy fluid i.e. dark energy component does not interact with barotropic matter. We obtain the present value of deceleration parameter and age of universe as [Formula: see text] and 13.329 Gyrs respectively. The analysis of [Formula: see text] and jerk parameters concur that the derived model approaches [Formula: see text]CDM universe at late time. We find that estimated values of Hubble’s constant and energy density parameters have pretty consistency with their corresponding values, obtained by recent observations of WMAP and PLANCK collaborations. It is also observed that the universe shows signature flipping from decelerating to accelerating phase for transitional red-shift value [Formula: see text] in past.
Effectiveness of Stroke Early Supported Discharge
Authors: Fisher Rebecca J. , Byrne Adrian , Chouliara Niki , Lewis Sarah , Paley Lizz , Hoffman Alex , Rudd Anthony , Robinson Thompson , Langhorne Peter , Walker Marion F. 
Published: July 2020

Background:
Implementation of stroke early supported discharge (ESD) services has been recommended in many countries’ clinical guidelines, based on clinical trial evidence. This is the first observational study to investigate the effectiveness of ESD service models operating in real-world conditions, at scale.

Methods and Results:
Using historical prospective data from the United Kingdom Sentinel Stroke National Audit Programme (January 1, 2016–December 31, 2016), measures of ESD effectiveness were “days to ESD” (number of days from hospital discharge to first ESD contact; n=6222), “rehabilitation intensity” (total number of treatment days/total days with ESD; n=5891), and stroke survivor outcome (modified Rankin scale at ESD discharge; n=6222). ESD service models (derived from Sentinel Stroke National Audit Programme postacute organizational audit data) were categorized with a 17-item score, reflecting adoption of ESD consensus core components (evidence-based criteria). Multilevel modeling analysis was undertaken as patients were clustered within ESD teams across the Midlands, East, and North of England (n=31). A variety of ESD service models had been adopted, as reflected by variability in the ESD consensus score. Controlling for patient characteristics and Sentinel Stroke National Audit Programme hospital score, a 1-unit increase in ESD consensus score was significantly associated with a more responsive ESD service (reduced odds of patient being seen after ≥1 day of 29% [95% CI, 1%–49%] and increased treatment intensity by 2% [95% CI, 0.3%–4%]). There was no association with stroke survivor outcome measured by the modified Rankin Scale.

Conclusions:
This study has shown that adopting defined core components of ESD is associated with providing a more responsive and intensive ESD service. This shows that adherence to evidence-based criteria is likely to result in a more effective ESD service as defined by process measures.

Registration:

URL:
http://www.isrctn.com/
; Unique identifier: ISRCTN15568163.
Persistent Renin‐Angiotensin System Sensitization Months After Body Weight Recovery From Severe Food Restriction in Female Fischer Rats
Authors: de Souza Aline M. A. , Ji Hong , Wu Xie , Sandberg Kathryn , West Crystal A. 
Published: July 2020

Background
Prior exposure to periods of severe food restriction (sFR) is associated with increased risk of developing hypertension and cardiovascular disease later in life.

Methods and Results

To investigate the mechanism of these long‐term adverse effects of sFR, 4‐month‐old female Fischer rats were divided in 2 groups and maintained on a normal diet ad libitum (control) or on an sFR diet with 60% reduction in daily food intake for 2 weeks that resulted in a 15% reduction in body weight. After the 2‐week sFR period ended, both groups received normal chow ad libitum for 3 months. Within 2 weeks after refeeding was initiated in the sFR group, body weight was restored to control levels; however, plasma angiotensinogen (1.3‐fold;
P
<0.05), Ang‐[1‐8] (2.0‐fold;
P
<0.05), and angiotensin‐converting enzyme activity (1.1‐fold;
P
<0.01) were all elevated 3 months after refeeding. Angiotensin type 1 receptor activity was also increased as evidenced by augmented pressor responses to angiotensin‐[1‐8] (
P
<0.01) and depressor responses to the angiotensin type 1 receptor antagonist, losartan (
P
<0.01) in the sFR group.

Conclusions
These results indicate that sensitization of the renin‐angiotensin system persisted months after the sFR period ended. These findings may have implications for women who voluntarily or involuntarily experience an extended period of sFR and thus may be at increased risk of developing cardiovascular disease through sensitization of the renin‐angiotensin system even though their body weight, mean arterial pressure, and heart rate appear normal.
Tropical Pacific Surface Wind Energy Spectra and Coherence: Basinwide Observations and Their Observing System Implications
Authors: Chiodi Andrew M. , Harrison D. E. 
Published: July 2020

AbstractThe tropical Pacific moored-buoy array spacing was based on wind coherence scales observed from low-lying islands in the western-central tropical Pacific. Since the array was deployed across the full basin in the mid-1990s, winds from the array have proven critical to accurately monitoring for decadal-scale changes in tropical Pacific winds and identifying spurious trends in wind analysis products used to monitor for long-term change. The array observations have also greatly advanced our ability to diagnostically model (hindcast) and thereby better understand the observed development of central Pacific sea surface temperature anomaly development associated with El Niño and La Niña events, although the eastern equatorial Pacific is not yet accurately hindcast. The original array-design assumptions that the statistics calculated from the western-central Pacific island records are representative of open-ocean conditions and other regions of the tropical Pacific have not been thoroughly reexamined. We revisit these assumptions using the basinwide wind observations provided by the array and find that key wind statistics change across the tropical Pacific basin in ways that could not be determined from the original island wind study. The island results provided a best-case answer for mooring zonal spacing with minimally redundant coherence between adjacent buoys. Buoy-observed meridional coherence scales are longer than determined from the islands. Enhanced zonal sampling east of 140°W and west of 180° is needed to obtain minimal redundancy (optimal spacing). Reduced meridional sampling could still yield minimal redundancy for wind and wind stress fields over the ocean waveguide.