bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dehydroxymethylepoxyquinomicin, a novel nuclear factor-κB inhibitor, prevents the development of cyclosporine A nephrotoxicity in a rat model
Authors: Morita Shinya , Shinoda Kazunobu , Yoshida Tadashi , Shimoda Masayuki , Kanno Yoshihiko , Mizuno Ryuichi , Kono Hidaka , Asanuma Hiroshi , Nakagawa Ken , Umezawa Kazuo , Oya Mototsugu 
Published: August 2020

AbstractBackgroundCyclosporine A (CsA) is an essential immunosuppressant in organ transplantation. However, its chronic nephrotoxicity is an obstacle to long allograft survival that has not been overcome. Nuclear factor-κB (NF-κB) is activated in the renal tissue in CsA nephropathy. In this study, we aimed to investigate the effect of the specific NF-κB inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ), in a rat model of CsA nephrotoxicity.MethodsWe administered CsA (15 mg/kg) daily for 28 days to Sprague-Dawley rats that underwent 5/6 nephrectomy under a low-salt diet. We administered DHMEQ (8 mg/kg) simultaneously with CsA to the treatment group, daily for 28 days and evaluated its effect on CsA nephrotoxicity.ResultsDHMEQ significantly inhibited NF-κB activation and nuclear translocation due to CsA treatment. Elevated serum urea nitrogen and creatinine levels due to repeated CsA administration were significantly decreased by DHMEQ treatment (serum urea nitrogen in CsA + DHMEQ vs CsA vs control, 69 ± 6.4 vs 113.5 ± 8.8 vs 43.1 ± 1.1 mg/dL, respectively,p < 0.0001; serum creatinine in CsA + DHMEQ vs CsA vs control, 0.75 ± 0.02 vs 0.91 ± 0.02 vs 0.49 ± 0.02 mg/dL, respectively, p < 0.0001), and creatinine clearance was restored in the treatment group (CsA + DHMEQ vs CsA vs control, 2.57 ± 0.09 vs 1.94 ± 0.12 vs 4.61 ± 0.18 ml/min/kg, respectively, p < 0.0001). However, DHMEQ treatment did not alter the inhibitory effect of CsA on urinary protein secretion. The development of renal fibrosis due to chronic CsA nephrotoxicity was significantly inhibited by DHMEQ treatment (CsA + DHMEQ vs CsA vs control, 13.4 ± 7.1 vs 35.6 ± 18.4 vs 9.4 ± 5.4%, respectively, p < 0.0001), and these results reflected the results of renal functional assessment. DHMEQ treatment also had an inhibitory effect on the increased expression of chemokines, monocyte chemoattractant protein-1, and chemokine (c-c motif) ligand 5 due to repeated CsA administration, which inhibited the infiltration of macrophages and neutrophils into the renal tissue.ConclusionsThese findings suggest that DHMEQ treatment in combination therapy with CsA-based immunosuppression is beneficial to prevent the development of CsA-induced nephrotoxicity.
Method development for acetyl octapeptide-3 analysis by liquid chromatography-tandem mass spectrometry
Authors: Ji Moongi , Lee Hyeon-Seong , Kim Youngbae , Seo Chan , Choi Subin , Oh Songjin , Min Jeuk , Park Hyung-Jin , Kim Jung Dong , Jeong Do Hyeon , Paik Man-Jeong 
Published: August 2020

Abstract
Background
Acetyl octapeptide-3 (SNAP-8) is an antiaging peptide that is more effective than acetyl hexapeptide-3, which is more stable than botulinum toxin and effectively relieves facial wrinkles. Products containing SNAP-8 such as patch are producing, but analytical method has not been reported to determination of SNAP-8.

Method
Mobile phase, collision energy, and desolvation line temperature were optimized, and mass spectral data set for SNAP-8 was newly constructed by liquid chromatography-triple quadrupole mass spectrometer in multiple reaction monitoring mode.

Results
The developed method showed good linearity (r ≥ 0.9971) with limit of quantification of 0.0125 ng/mL, repeatability (% relative standard deviation = 0.02 to 0.12) and accuracy (% relative error = − 1.68 to 1.44) under optimal conditions. This method was successfully applied to a biodegradable microneedle patch loaded with SNAP-8.

Conclusion
The present method for the quantification of SNAP-8 may be useful for quality control in the cosmetic fields.
BLAST-QC: automated analysis of BLAST results
Authors: Torkian Behzad , Hann Spencer , Preisner Eva , Norman R. Sean 
Published: August 2020

AbstractBackgroundThe Basic Local Alignment Search Tool (BLAST) from NCBI is the preferred utility for sequence alignment and identification for bioinformatics and genomics research. Among researchers using NCBI’s BLAST software, it is well known that analyzing the results of a large BLAST search can be tedious and time-consuming. Furthermore, with the recent discussions over the effects of parameters such as ‘-max_target_seqs’ on the BLAST heuristic search process, the use of these search options are questionable. This leaves using a stand-alone parser as one of the only options of condensing these large datasets, and with few available for download online, the task is left to the researcher to create a specialized piece of software anytime they need to analyze BLAST results. The need for a streamlined and fast script that solves these issues and can be easily implemented into a variety of bioinformatics and genomics workflows was the initial motivation for developing this software.ResultsIn this study, we demonstrate the effectiveness of BLAST-QC for analysis of BLAST results and its desirability over the other available options. Applying genetic sequence data from our bioinformatic workflows, we establish BLAST_QC’s superior runtime when compared to existing parsers developed with commonly used BioPerl and BioPython modules, as well as C and Java implementations of the BLAST_QC program. We discuss the ‘max_target_seqs’ parameter, the usage of and controversy around the use of the parameter, and offer a solution by demonstrating the ability of our software to provide the functionality this parameter was assumed to produce, as well as a variety of other parsing options. Executions of the script on example datasets are given, demonstrating the implemented functionality and providing test-cases of the program. BLAST-QC is designed to be integrated into existing software, and we establish its effectiveness as a module of workflows or other processes.ConclusionsBLAST-QC provides the community with a simple, lightweight and portable Python script that allows for easy quality control of BLAST results while avoiding the drawbacks of other options. This includes the uncertain results of applying the -max_target_seqs parameter or relying on the cumbersome dependencies of other options like BioPerl, Java, etc. which add complexity and run time when running large data sets of sequences. BLAST-QC is ideal for use in high-throughput workflows and pipelines common in bioinformatic and genomic research, and the script has been designed for portability and easy integration into whatever type of processes the user may be running.
Comparative evaluation of Salicornia bigelovii oil planted under different treatments
Authors: El-Araby R. , Rezk A. I. , El-Enin S. A. Abo , Nofal O. A. , El-Nasharty A. B. 
Published: August 2020

AbstractBackgroundAssessment of the oil % in a representative sample ofSalicorniabigeloviiseeds with different extraction methods using some of the organic solvents to reach the best economic one and determination of the effect ofS.bigeloviicultivation under different conditions of irrigation and fertilization on the percentage, composition, and nutrition value of oil were done. From the International Training Centre-Mariut, Alexandria, we obtained representative samples ofSalicornia bigeloviiseeds which are collected from the sea coast and 17 samples ofS.bigeloviiseeds which are subjected to different treatments of irrigation and fertilization. The oil % in a blank sample (B) was determined using different solvents (hexane, hexane-isopropanol, and ethanol) with variant methods (shaker, conventional, and ultrasonic). Oil % in 17 samples was evaluated by the ultrasonic probe technique using ethanol. The oil composition was determined in all samples by GC analysis. The phenolic and flavonoid contents in the residue were determined with the Folin-Ciocalteu reagent and colorimetric method, respectively.ResultsThe ethanol has higher extraction efficiency and the ultrasonic probe recovered higher oil % in the blank sample. Seventeen samples ofS.bigeloviicultivated under different combined treatments of fertilization and irrigation recorded oil % less than the blank sample. The fatty acids profile cleared that the oil of cultivated samples (17 samples) had power for biodiesel production with high ignition properties and good stability due to the high cetane number and a high percentage of palmitic acid. The nutrition value of the blank sample is higher than that of the other 17 samples. The polyunsaturated fatty acids in the blank sample represent > 63% of the total fatty acids.ConclusionThe different combined treatments of fertilization and irrigation ofS.bigeloviichanged the fatty acid profiles of all samples, palmitic, stearic, oleic, and linoleic acids were recognized as the most abundant saturated fatty acids. The total phenolic and flavonoid contents differed in the sample to another one but less than their contents in the blank sample.
Alternative uses for tannery wastes: a review of environmental, sustainability, and science
Authors: Rigueto Cesar Vinicius Toniciolli , Rosseto Marieli , Krein Daniela Dal Castel , Ostwald Bruna Elisangela Pessini , Massuda Lillian Avila , Zanella Bárbara Belém , Dettmer Aline 
Published: August 2020

Abstract
The manufacture of leather covers a wide productive chain and beyond contributes to the economic flow. The various stages of leather processing result in high volumes of solid waste. In Brazil, a daily generation of 375 tons of solid waste is estimated, and landfills are still the most used route to its destination. In this review, emphasis will be given to researches that have sought alternatives for the use of solid waste from the tannery industry. Among the main applications of solid tannery wastes, the following stand out production of adsorbent materials, biodiesel, biogas, biopolymers, applications in agriculture and other applications involving extraction/recovery of compounds of industrial/commercial interest, isolation of microorganisms and production of enzymes and applications in the animal diet. In each alternative of waste application, the technologies used, the opportunities, and the challenges faced are mentioned. We hope that this review can provide valuable information to promote the broad understanding of the possibilities that tannery solid wastes has for the development of biodegradable and agricultural products, wastewater treatment, extraction of compounds of industrial and commercial interest, among others.

Graphical abstract
Tolerance analysis of non-depolarizing double-pass polarimetry
Authors: Yu Yimin , Baba-Ali Nabila , Gallatin Gregg M. 
Published: August 2020

AbstractDouble-pass polarimetry measures the polarization properties of a sample over a range of polar angles and all azimuths. Here, we present a tolerance analysis of all the optical elements in both the calibration and measurement procedures to predict the sensitivities of the double-pass polarimeter. The calibration procedure is described by a Mueller matrix based on the eigenvalue calibration method (ECM) [1]. Our numerical results from the calibration and measurement in the Mueller matrix description with tolerances limited by systematic and stochastic noise from specifications of commercially available hardware components are in good agreement with previous experimental observations. Furthermore, by using the orientation Zernike polynomials (OZP) which are an extension of the Jones matrix formalism, similar to the Zernike polynomials wavefront expansion, the pupil distribution of the polarization properties of non-depolarizing samples under test are expanded. Using polar angles ranging up to 25∘, we predict a sensitivity of 0.5% for diattenuation and 0.3∘for retardance using the root mean square (RMS) of the corresponding OZP coefficients as a measure of the error. This numerical tool provides an approach for further improving the sensitivities of polarimeters via error budgeting and replacing sensitive components with those having better precision.
Is Subacute Thyroiditis an Underestimated Manifestation of SARS-CoV-2 Infection? Insights From a Case Series
Authors: Brancatella Alessandro , Ricci Debora , Cappellani Daniele , Viola Nicola , Sgrò Daniele , Santini Ferruccio , Latrofa Francesco 
Published: August 2020

Abstract

Context
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected more than 18 million people worldwide and the pandemic is still spreading. After the first case we reported, we observed 4 additional cases of subacute thyroiditis (SAT) related to SARS-CoV-2 infection.

Objectives
The objective of this work is to describe additional cases of SAT associated with SARS-CoV-2 infection to alert physicians that SAT may be a manifestation of SARS-CoV-2 infection.

Methods
We describe clinical, biochemical, and imaging features of 4 patients with SAT related to SARS-CoV-2 infection.

Results
All patients were female (age, 29-46 years). SAT developed 16 to 36 days after the resolution of coronavirus disease 2019 (COVID-19). Neck pain radiated to the jaw and palpitations were the main presenting symptoms and were associated with fever and asthenia. One patient was hospitalized because of atrial fibrillation. Thyroid function tests (available for 3 individuals) were suggestive of destructive thyroiditis, and inflammatory markers were high. At neck ultrasound the thyroid was enlarged, with diffuse and bilateral hypoechoic areas and (in 3 patients) absent vascularization at color Doppler. Symptoms disappeared a few days after commencement of treatment (prednisone in 3 patients and ibuprofen in 1). Six weeks after the onset of SAT, all patients were asymptomatic and inflammatory markers had returned to normal range. Two patients were euthyroid, whereas 2 were diagnosed with subclinical hypothyroidism.

Conclusions
SAT may be an underestimated manifestation of COVID-19. Clinicians should keep in mind the possible occurrence of SAT during and after SARS-CoV-2 infection.
Como permanecer relevante?
Authors: Wilner Adriana , Dos Santos Aline Lilian 
Published: August 2020

Nos anos 1950, a Fundação Getulio Vargas foi pioneira na criação de cursos de administração pública e de empresas no Brasil. Formou gerações que transformaram a gestão no país. Hoje, as escolas de administração, em conjunto, têm mais alunos do que qualquer outra faculdade. Luiz Artur Ledur Brito, diretor da Escola de Administração de Empresas de São Paulo da Fundação Getulio Vargas (FGV EAESP), ao refletir sobre a trajetória da escola, levanta a seguinte questão: “Como podemos ambicionar um impacto para a comunidade que relembre a relevância histórica que tivemos?”.Brito aponta, nesta entrevista à GV-executivo, dois caminhos para a FGV EAESP ermanecer influenciando as organizações e, de forma mais ampla, a sociedade brasileira. O primeiro é formar alunos que tenham, além de habilidades técnicas, a capacidade de interpretar, de forma ampla e estratégica, um mundo que se transforma rapidamente. O outro é não só gerar conhecimento, mas transformar esse conhecimento em recomendações que possam ser aplicadas em organizações públicas e privadas.
Liderança 360 graus
Authors: Wilner Adriana , Dos Santos Aline Lilian 
Published: August 2020

Growth and comfort do not coexist”. A frase, em inglês, está escrita na parede da sede da International Business Machines Corporation (IBM) para a América Latina, em um moderno prédio da Avenida Juscelino Kubitschek, em São Paulo. De autoria da chief executive officer (CEO) mundial da empresa, Ginni Rometty, a afirmaçãode que não é possível ficar em uma posição confortável para conseguir crescimento ilustra os desafios de quem atua no mercado de tecnologia.Primeira mulher a liderar a operação na América Latina, Ana Paula Assis está na IBM há 24 anos, desde que conseguiu entrar em um programa de estágio da companhia em sua cidade natal, Goiânia. Ter construído carreira na mesma empresa, no entanto, não foi nada monótono. A executiva viveu enormes transformações ao longo de sua trajetória profissional, como a IBM passar de uma empresa de infraestrutura e hardware para um negócio de serviços e software, inserindo-se em mercados como o de computadores pessoais e, mais recentemente, despontando em inteligência artificial, blockchain e quantum computing. “É um constante processo de reinvenção e transformação. Na nossa indústria, não tem perdão; se você parar, vai morrer”, diz Ana Paula nesta entrevista exclusiva à GV-executivo.
DNA Gyrase and Topoisomerase IV Mutations and their effect on Quinolones Resistant Proteus mirabilis among UTIs Patients
Authors: Abdelkreem Randa Hassan , Yousuf Amjad Mohammed , Elmekki Miskelyemen Abdelatti , Elhassan Mogahid Mohammed 
Published: August 2020

Objective: This study aimed to highlight the importance of mutations within Proteus mirabilis genome that are related to fluoroquinolone resistance.
Methods: This is a cross sectional study performed in different teaching hospitals in Khartoum State from June 2016 to May 2017. A total of (120) P mirabilis isolates from patients with symptoms of UTIs attending different hospitals in Khartoum State were examined. First, modified Kurby Bauer method was performed for phenotypical detection of resistant isolates. Then polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) followed by sequencing were applied for detection of mutations in GyrA, GyrB, ParC and ParE genes of isolates.
Results: P. mirabilis showed 30% resistance to ciprofloxacin. All samples revealed mutation at (serine 83) of GyrA and (serine 84) of ParC by Hinf1 restriction endonuclease digestion. Sequencing was performed for 12 samples. For each gene, two resistant and one susceptible strains were randomly selected. The mutations associated with ciprofloxacin resistant P. mirabilis were as follows; (1/3) GyrA (Ser 83 to Ile) and (2/3) ParC (Ser 81 to Ile). Also it revealed silent mutations at codons of GyrB 474 leucine (3/3), 585 valine (2/3), 612 histidine (1/3) and 639 asparagine (1/3) and ParE 469 isoleucine (2/3), 531 aspartic (2/3) and 533 glycine (1/3).
Conclusions: Ciprofloxacin resistance in P. mirabilis could be monitored through detection of mutations within DNA gyrase (encoded by gyrA and gyrB) and topoisomerase IV (encoded by parC and parE).
doi: https://doi.org/10.12669/pjms.36.6.2207
How to cite this:Abdelkreem RH, Yousuf AM, Elmekki MA, Elhassan MM. DNA Gyrase and Topoisomerase IV Mutations and their effect on Quinolones Resistant Proteus mirabilis among UTIs Patients. Pak J Med Sci. 2020;36(6):1234-1240. doi: https://doi.org/10.12669/pjms.36.6.2207
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Case Report: Cryptococcal meningitis in Hodgkin’s Lymphoma patient receiving brentuximab-vedotin therapy
Authors: Cunha Pereira Tatiana , Rb-Silva Rita , Félix Soares Rita , Domingues Nelson , Mariz José 
Published: August 2020

Cryptococcus neoformans infections occur mostly in immunodeficient individuals, being the most common opportunistic fungal central nervous system (CNS) infection in HIV seropositive patients. Moreover, other conditions affecting host immunity, such as hematologic malignancies, organ transplantation and immunosuppressive drugs are implicated as risk factors. The authors present a case of a 48-year-old male with Hodgkin Lymphoma for 26 years and submitted to several lines of treatment, diagnosed with cryptococcal meningitis while on therapy with brentuximab. The patient presented with positive cerebral spinal fluid (CSF) cryptococcal antigen plus positive blood cultures. He was put under induction antifungal treatment with liposomal amphotericin B and flucytosine, as well as corticosteroid therapy with dexamethasone with headache improvement and a favorable clinical evolution. There are no reported cases of cryptococcal meningoencephalitis under CD30-directed monoclonal antibody. Furthermore, this case illustrates the risk of Cryptococcus neoformans infection in immunocompromising conditions other than HIV, underlining the need of considering this differential diagnosis when physicians face an opportunistic neuroinfection.
ALGUMAS INFLUÊNCIAS TEÓRICAS DETERMINANTES DO CONCEITO DE DESENVOLVIMENTO EM JOSUÉ DE CASTRO: UM PRELÚDIO
Authors: Ribeiro Junior José Arnaldo Dos Santos 
Published: August 2020

Investiga algumas influências teóricas determinantes do conceito de desenvolvimento em Josué de Castro (1908-1973), caracterizando sua origem histórica, suas principais influências teóricas, hipóteses básicas e proposições fundamentais. Particularmente, procura-se reconstituir historicamente e dialeticamente a trajetória teórica do escrito: Crise social e desenvolvimento econômico do mundo. Os aspectos do subdesenvolvimento em Josué foram primeiramente estudados por Giuseppe Di Taranto (1993 [1978]) que apontou a dupla influência do dualismo econômico – através da obra do economista William Arthur Lewis (1915-1991) e da Comissão Econômica para América Latina e Caribe (CEPAL) – e do neomarxismo do também economista André Gunder Frank (1929-2005) na obra de Castro. Embora Di Taranto conheça o texto Crise social e desenvolvimento econômico do mundo ele não se debruça sob o mesmo. Esta lacuna na importante obra de Giuseppe anuvia as insignes contribuições teóricas advindas do economista Ragnar Nurkse (1907-1959), Vladimir Ilyich Ulyanov – Lênin – (1870-1924) e Karl Marx (1818-1883). Por conseguinte, aponta-se a contradição entre as citadas influências teóricas que resultarão num desenlace no mínimo inesperado: Josué de Castro – defensor dos camponeses e operários, crítico do colonialismo/imperialismo – acaba por suster o capitalismo enquanto saída para os problemas dos países subdesenvolvidos.
High-performance electron-blocking-layer-free deep ultraviolet light-emitting diodes implementing a strip-in-a-barrier structure
Authors: Velpula Ravi Teja , Jain Barsha , Velpula Swetha , Nguyen Hoang-Duy , Nguyen Hieu Pham Trung 
Published: August 2020

In this Letter, the electron-blocking-layer (EBL)-free AlGaN ultraviolet (UV) light-emitting diodes (LEDs) using a strip-in-a-barrier structure have been proposed. The quantum barrier (QB) structures are systematically engineered by integrating a 1 nm intrinsic

A
l

x

G
a

(
1
−
x
)

N

strip into the middle of QBs. The resulted structures exhibit significantly reduced electron leakage and improved hole injection into the active region, thus generating higher carrier radiative recombination. Our study shows that the proposed structure improves radiative recombination by

∼

220

%

, reduces electron leakage by

∼

11

times, and enhances optical power by

∼

225

%

at 60 mA current injection compared to a conventional AlGaN EBL LED structure. Moreover, the EBL-free strip-in-a-barrier UV LED records the maximum internal quantum efficiency (IQE) of

∼

61.5

%

which is

∼

72

%

higher, and IQE droop is

∼

12.4

%

, which is

∼

333

%

less compared to the conventional AlGaN EBL LED structure at

∼

284.5

n
m

wavelength. Hence, the proposed EBL-free AlGaN LED is the potential solution to enhance the optical power and produce highly efficient UV emitters.