bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nonverbal leadership emergence in walking groups
Authors: Lombardi Maria , Warren William H. , di Bernardo Mario 
Published: November 2020

AbstractThe mechanisms underlying the emergence of leadership in multi-agent systems are under investigation in many areas of research where group coordination is involved. Nonverbal leadership has been mostly investigated in the case of animal groups, and only a few works address the problem in human ensembles, e.g. pedestrian walking, group dance. In this paper we study the emergence of leadership in the specific scenario of a small walking group. Our aim is to propose a rigorous mathematical methodology capable of unveiling the mechanisms of leadership emergence in a human group when leader or follower roles are not designated a priori. Two groups of participants were asked to walk together and turn or change speed at self-selected times. Data were analysed using time-dependent cross correlation to infer leader-follower interactions between each pair of group members. The results indicate that leadership emergence is due both to contextual factors, such as an individual’s position in the group, and to personal factors, such as an individual’s characteristic locomotor behaviour. Our approach can easily be extended to larger groups and other scenarios such as team sports and emergency evacuations.
Field pea (Pisum sativum L.) shows genetic variation in phosphorus use efficiency in different P environments
Authors: Powers Sarah , Mirsky Emily , Bandaranayake Anuruddha , Thavarajah Pushparajah , Shipe Emerson , Bridges William , Thavarajah Dil 
Published: November 2020

AbstractField pea is important to agriculture as a nutritionally dense legume, able to fix nitrogen from the atmosphere and supply it back to the soil. However, field pea requires more phosphorus (P) than other crops. Identifying field pea cultivars with high phosphorus use efficiency (PUE) is highly desirable for organic pulse crop biofortification. This study identified field pea accessions with high PUE by determining (1) the variation in P remobilization rate, (2) correlations between P and phytic acid (PA), and (3) broad-sense heritability estimates of P concentrations. Fifty field pea accessions were grown in a completely randomized design in a greenhouse with two replicates under normal (7551 ppm) and reduced (4459 ppm) P fertilizer conditions and harvested at two time points (mid-pod and full-pod). P concentrations ranged from 332 to 9520 ppm under normal P and from 83 to 8473 ppm under reduced P conditions across all tissues and both time points. Field pea accessions showed variation in remobilization rates, with PI 125840 and PI 137119 increasing remobilization of P under normal P conditions. Field pea accessions PI 411142 and PI 413683 increased P remobilization under the reduced P treatment. No correlation was evident between tissue P concentration and seed PA concentration (8–61 ppm). Finally, seed P concentration under limited P conditions was highly heritable (H2 = 0.85), as was mid-pod lower leaf P concentrations under normal P conditions (H2 = 0.81). In conclusion, breeding for PUE in field pea is possible by selecting for higher P remobilization accessions in low P soils with genetic and location sourcing.
Glucocorticoid-dependent transcription in skin requires epidermal expression of the glucocorticoid receptor and is modulated by the mineralocorticoid receptor
Authors: Sevilla Lisa M. , Bigas Judit , Chiner-Oms Álvaro , Comas Iñaki , Sentandreu Vicente , Pérez Paloma 
Published: November 2020

AbstractGlucocorticoid (GC) actions are mediated through two closely related ligand-dependent transcription factors, the GC receptor (GR) and the mineralocorticoid receptor (MR). Given the wide and effective use of GCs to combat skin inflammatory diseases, it is important to understand the relative contribution of these receptors to the transcriptional response to topical GCs. We evaluated the gene expression profiles in the skin of mice with epidermal-specific loss of GR (GREKO), MR (MREKO), or both (double KO; DKO) in response to dexamethasone (Dex). The overall transcriptional response was abolished in GREKO and DKO skin suggesting dependence of the underlying dermis on the presence of epidermal GR. Indeed, the observed dermal GC resistance correlated with a constitutive decrease in GR activity and up-regulation of p38 activity in this skin compartment. Upon Dex treatment, more than 90% of differentially expressed genes (DEGs) in CO overlapped with MREKO. However, the number of DEGs was fourfold increased and the magnitude of response was higher in MREKO vs CO, affecting both gene induction and repression. Taken together our data reveal that, in the cutaneous transcriptional response to GCs mediated through endogenous receptors, epidermal GR is mandatory while epidermal MR acts as a chief modulator of gene expression.
Authentification of fruit spirits using HS-SPME/GC-FID and OPLS methods
Authors: Bajer Tomáš , Hill Martin , Ventura Karel , Bajerová Petra 
Published: November 2020

AbstractThis research provides an accurate description of the origin for fruit spirits. In total, 63 samples of various kinds of fruit spirits (especially from apples, pears, plums, apricots and mirabelle) were analysed using headspace-solid phase microextraction and gas chromatography with flame-ionization detector. Obtained volatile profiles were treated and analysed by multivariate regression with a reduction of dimensionality-orthogonal projections to latent structure for the classification of fruit spirits according to their fruit of origin. Basic result of statistical analysis was the differentiation of spirits to groups with respect to fruit kind. Tested kinds of fruit spirits were strictly separated from each other. The selection was achieved with a specificity of 1.000 and a sensitivity of 1.000 for each kind of spirit. The statistical model was verified by an external validation. Hierarchical cluster analysis (calculation of distances by Ward’s method) showed a similarity of volatile profiles of pome fruit spirits (apple and pear brandies) and stone fruit spirits (especially mirabelle and plum brandies).
Transformation of nonencapsulated Streptococcus pneumoniae during systemic infection
Authors: Bradshaw Jessica L. , Rafiqullah Iftekhar M. , Robinson D. Ashley , McDaniel Larry S. 
Published: November 2020

AbstractStreptococcus pneumoniae (pneumococcus) is a principal cause of bacterial middle ear infections, pneumonia, and meningitis. Capsule-targeted pneumococcal vaccines have likely contributed to increased carriage of nonencapsulated S. pneumoniae (NESp). Some NESp lineages are associated with highly efficient DNA uptake and transformation frequencies. However, NESp strains lack capsule that may increase disease severity. We tested the hypothesis that NESp could acquire capsule during systemic infection and transform into more virulent pneumococci. We reveal that NESp strains MNZ67 and MNZ41 are highly transformable and resistant to multiple antibiotics. Natural transformation of NESp when co-administered with heat-killed encapsulated strain WU2 in a murine model of systemic infection resulted in encapsulation of NESp and increased virulence during bacteremia. Functional capsule production increased the pathogenic potential of MNZ67 by significantly decreasing complement deposition on the bacterial surface. However, capsule acquisition did not further decrease complement deposition on the relatively highly pathogenic strain MNZ41. Whole genome sequencing of select transformants demonstrated that recombination of up to 56.7 kbp length occurred at the capsule locus, along with additional recombination occurring at distal sites harboring virulence-associated genes. These findings indicate NESp can compensate for lack of capsule production and rapidly evolve into more virulent strains.
Population genetic portrait of Pakistani Lahore-Christians based on 32 STR loci
Authors: Rubab Aqsa , Shafique Muhammad , Javed Faqeeha , Saleem Samia , Zahra Fatima Tuz , McNevin Dennis , Shahid Ahmad Ali 
Published: November 2020

AbstractPhylogenetic relationship and the population structure of 500 individuals from the Christian community of Lahore, Pakistan, were examined based on 15 autosomal short tandem repeats (STRs) using the AmpFℓSTR Identifiler Plus PCR Amplification Kit and our previously published Y-filer kit data (17 Y-STRs) of same samples. A total of 147 alleles were observed in 15 loci and allele 11 at the TPOX locus was the most frequent with frequency value (0.464). The data revealed that the Christian population has unique genetic characteristics with respect to a few unusual alleles and their frequencies relative to the other Pakistani population. Significant deviations from Hardy–Weinberg equilibrium were found at two loci (D13S317, D18S51) after Boneferroni’s correction (p ≤ 0.003). The combined power of discrimination, combined power of exclusion and cumulative probability of matching were 0.999999999999999978430815060354, 0.999995039393942 and 2.15692 × 10−17, respectively. On the bases of genetic distances, PCA, phylogenetic and structure analysis Lahore-Christians appeared genetically more associated to south Asian particularly Indian populations like Tamil, Karnataka, Kerala and Andhra Pradesh than rest of global populations.
Morphological and physiological changes in Lentilactobacillus hilgardii cells after cold plasma treatment
Authors: Niedźwiedź Iwona , Juzwa Wojciech , Skrzypiec Krzysztof , Skrzypek Tomasz , Waśko Adam , Kwiatkowski Michał , Pawłat Joanna , Polak-Berecka Magdalena 
Published: November 2020

AbstractAtmospheric cold plasma (ACP) inactivation of Lentilactobacillus hilgardii was investigated. Bacteria were exposed to ACP dielectric barrier discharge with helium and oxygen as working gases for 5, 10, and 15 min. The innovative approach in our work for evaluation of bacterial survival was the use in addition to the classical plate culture method also flow cytometry which allowed the cells to be sorted and revealed different physiological states after the plasma treatment. Results showed total inhibition of bacterial growth after 10-min of ACP exposure. However, the analysis of flow cytometry demonstrated the presence of 14.4% of active cells 77.5% of cells in the mid-active state and 8.1% of dead cells after 10 min. In addition, some of the cells in the mid-active state showed the ability to grow again on culture medium, thus confirming the hypothesis of induction of VBNC state in L .hilgardii cells by cold plasma. In turn, atomic force microscopy (AFM) which was used to study morphological changes in L. hilgardii after plasma treatment at particular physiological states (active, mid-active, dead), showed that the surface roughness of the mid-active cell (2.70 ± 0.75 nm) was similar to that of the control sample (2.04 ± 0.55 nm). The lack of considerable changes on the cell surface additionally explains the effective cell resuscitation. To the best of our knowledge, AFM was used for the first time in this work to analyze cells which have been sorted into subpopulations after cold plasma treatment and this is the first work indicating the induction of VBNC state in L. hilgardii cells after exposure to cold plasma.
Performance controlled via surface oxygen-vacancy in Ti-based oxide catalyst during methyl oleate epoxidation
Authors: Praserthdam Supareak , Rittiruam Meena , Maungthong Kanokpon , Saelee Tinnakorn , Somdee Siriwimol , Praserthdam Piyasan 
Published: November 2020

AbstractThe catalytic performance with high conversion and high selectivity of Ti-based oxide catalysts have been widely investigated. Besides, stability, which is an essential parameter in the industrial process, lacked fundamental understanding. In this work, we combined computational and experimental techniques to provide insight into the deactivation of P25 and TS-1 Ti-based oxide catalysts during the methyl oleate (MO) epoxidation. The considered deactivation mechanisms are fouling and surface oxygen vacancy (OV). The fouling causes temporary catalyst deactivation through active site blockage but can be removed via calcination in air at high temperature. However, in this work, the OV formation plays an important role in the overall performance of the spent catalyst as the deactivated catalyst after regeneration, cannot be restored to the initial activity. Also, the effects of OV in spent catalysts caused (i) the formation of more Ti3+ species on the surface as evident by XPS and Bader charge analysis, (ii) the activity modification of the active region on the catalyst surface as the reduction in energy gap (Eg) occurred from the formation of the interstates observed in the density of states profiles of spent catalyst modeled by the O-vacant P25 and TS-1 models. This reduction in Eg affects directly the strength of Ti–OOH active site and MO bonding, in which high binding energy contributes to a low conversion because the MO needed an O atom from Ti–OOH site to form the methyl-9,10-epoxy stearate. Hence, the deactivation of the Ti-based oxide catalysts is caused not only by the insoluble by-products blocking the active region but also mainly from the OV. Note that the design of reactive and stable Ti-based oxide catalysts for MO epoxidation needed strategies to prevent OV formation that permanently deactivated the active region. Thus, the interrelation and magnitude between fouling and OV formation on catalyst deactivation will be investigated in future works.
The study of automatic machine learning base on radiomics of non-focus area in the first chest CT of different clinical types of COVID-19 pneumonia
Authors: Tan Hui-Bin , Xiong Fei , Jiang Yuan-Liang , Huang Wen-Cai , Wang Ye , Li Han-Han , You Tao , Fu Ting-Ting , Lu Ran , Peng Bi-Wen 
Published: November 2020

AbstractTo explore the possibility of predicting the clinical types of Corona-Virus-Disease-2019 (COVID-19) pneumonia by analyzing the non-focus area of the lung in the first chest CT image of patients with COVID-19 by using automatic machine learning (Auto-ML). 136 moderate and 83 severe patients were selected from the patients with COVID-19 pneumonia. The clinical and laboratory data were collected for statistical analysis. The texture features of the Non-focus area of the first chest CT of patients with COVID-19 pneumonia were extracted, and then the classification model of the first chest CT of COVID-19 pneumonia was constructed by using these texture features based on the Auto-ML method of radiomics, The area under curve(AUC), true positive rate(TPR), true negative rate (TNR), positive predictive value(PPV) and negative predictive value (NPV) of the operating characteristic curve (ROC) were used to evaluate the accuracy of the first chest CT image classification model in patients with COVID-19 pneumonia. The TPR, TNR, PPV, NPV and AUC of the training cohort and test cohort of the moderate group and the control group, the severe group and the control group, the moderate group and the severe group were all greater than 95% and 0.95 respectively. The non-focus area of the first CT image of COVID-19 pneumonia has obvious difference in different clinical types. The AUTO-ML classification model of Radiomics based on this difference can be used to predict the clinical types of COVID-19 pneumonia.
Identification prognosis-associated immune genes in colon adenocarcinoma
Authors: Miao Yandong , Wang Jiangtao , Ma Xueping , Yang Yuan , Mi Denghai 
Published: November 2020

AbstractColon adenocarcinoma (COAD) is one of the most prevalent malignant tumors worldwide. Immune genes (IGs) have a considerable correlation with tumor initiation and prognosis. The present paper aims to identify the prognosis value of IGs in COAD and conduct a prognosis model for clinical utility. Gene expression data of COAD were downloaded from The Cancer Genome Atlas (TCGA), screening and analyzing differentially expressed IGs by bioinformatics. Core genes were screened by univariate and multivariate Cox regression analyses. Survival analysis was appraised by the Kaplan–Meier method and the log-rank test. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Gene Set Enrichment Analysis (GSEA) were used to identify IGs’ relevant signal pathways. We predicted the overall survival (OS) by nomogram. Finally, a prognosis model was conducted based on 12 IGs (SLC10A2, CXCL3, NOX4, FABP4, ADIPOQ, IGKV1-33, IGLV6-57, INHBA, UCN, VIP, NGFR, and TRDC). The risk score was an independent prognostic factor, and a nomogram could accurately predict the OS of individual COAD patients. These results were validated in GSE39582, GSE12945, and GSE103479 cohorts. Functional enrichment analysis demonstrated that these IGs are mainly enriched in hormone secretion, hormone transport, lipid transport, cytokine–cytokine receptor interaction, and peroxisome proliferators-activated receptor signaling pathway. In summary, the risk score is an independent prognostic biomarker. We also excavated several IGs related to COAD’s survival and maybe potential biomarkers for COAD diagnosis and treatment.
Activity time series of old stars from late F to early K
Authors: Meunier N. , Lagrange A.-M. , Borgniet S. 
Published: November 2020

Context. Stellar activity strongly affects and may prevent the detection of Earth-mass planets in the habitable zone of solar-type stars with radial velocity technics. Astrometry is in principle less sensitive to stellar activity because the situation is more favourable: the stellar astrometric signal is expected to be fainter than the planetary astrometric signal compared to radial velocities.
Aims. We quantify the effect of stellar activity on high-precision astrometry when Earth-mass planets are searched for in the habitable zone around old main-sequence solar-type stars.
Methods. We used a very large set of magnetic activity synthetic time series to characterise the properties of the stellar astrometric signal. We then studied the detectability of exoplanets based on different approaches: first based on the theoretical level of false positives derived from the synthetic time series, and then with blind tests for old main-sequence F6-K4 stars.
Results. The amplitude of the signal can be up to a few times the solar value depending on the assumptions made for activity level, spectral type, and spot contrast. The detection rates for 1 MEarth planets are very good, however, with extremely low false-positive rates in the habitable zone for stars in the F6-K4 range at 10 pc. The standard false-alarm probability using classical bootstrapping on the time series strongly overestimates the false-positive level. This affects the detection rates.
Conclusions. We conclude that if technological challenges can be overcome and very high precision is reached, astrometry is much more suitable for detecting Earth-mass planets in the habitable zone around nearby solar-type stars than radial velocity, and detection rates are much higher for this range of planetary masses and periods when astrometric techniques are used than with radial velocity techniques.
Konzeptentwicklung zum Materialtransport per Drohne zwischen den drei Kreisspitalstiftungskliniken im Kreis Neu-Ulm
Authors: Stitz Marcel S.A. , Swoboda Walter , Holl Felix 
Published: November 2020

ZusammenfassungDie Logistik stellt Klinken im ländlichen Raum mit mehreren Standorten vor große organisatorische und finanzielle Herausforderungen. Mit einer Machbarkeitsstudie wurde der Einsatz von drohnengetriebenem Materialtransport untersucht. Die wirtschaftliche und rechtliche Machbarkeit eines drohnengetriebenen Materialtransportes zwischen drei Kliniken in Bayern gestaltet sich schwierig. Föderalismus, komplexe Gesetzgebung und geringe Transportkapazitäten verhindern einen sinnvollen Einsatz der Technologie zum Materialtransport im beleuchteten Szenario.
Antiproliferative Effects of Pterodon pubescens Extract and Isolated Diterpenes in HaCaT Cells
Authors: Basting Rosanna Tarkany , de Oliveira Sousa Ilza Maria , Butterweck Veronika , Foglio Mary Ann 
Published: November 2020

Abstract
Pterodon pubescens fruits are popularly used because of their analgesic and anti-inflammatory actions, which are attributed to the isolated compounds with a vouacapan skeleton. This work aimed to evaluate the antiproliferative and anti-inflammatory effects of a P. pubescens fruit dichloromethane extract and the vouacapan diterpene furan isomerʼs mixture (1 : 1) (6α-hydroxy-7β-acetoxy-vouacapan-17β-oate methyl ester and 6α-acetoxy-7β-hydroxy-vouacapan-17β-oate methyl ester isomers) in HaCaT cells using the cell migration and the BrDU incorporation assay. Levels of IL-8 were measured by ELISA after TNF-α stimulation. HPLC/DAD analysis of the extract revealed the expressive presence of vouacapan diterpene furan isomerʼs mixture. P. pubescens extract (1.5625 – 25 µg/mL) and vouacapan diterpene furan isomerʼs mixture (3.125 – 50 µM) inhibited cell proliferation as indicated by a decreased
BrdU-incorporation. For the evaluation of cell migration, time-lapse microscopy was used. P. pubescens presented inhibition on cell migration at all concentrations tested (3.125 – 12.5 µg/mL), whereas for the VDFI mixture, the inhibition was only observed at the highest concentrations (12.5 and 25 µM) tested. Furthermore P. pubescens extract and vouacapan diterpene furan isomerʼs mixture significantly decreased IL-8 levels. Our results showed antiproliferative and anti-inflammatory effects on HaCaT cells treated with the extract and the vouacapan isomerʼs mixture, without affecting cell viability. These activities could be attributed to the voucapan molecular structures. In conclusion, topical products developed of P. pubescens extract or the voucapan isomerʼs mixture should be further studied as a potential product for local treatment against hyperproliferative lesions as in psoriasis vulgaris, representing an alternative treatment approach.
Synthesis of Optically Active Maresin 2 and Maresin 2n-3 DPA
Authors: Ogawa Narihito , Amano Takahito , Kobayashi Yuichi 
Published: November 2020

AbstractMaresins are among the most potent antiinflammatory lipid metabolites. We report stereoselective syntheses of maresin 2 and maresin 2n-3 DPA. The anti-diol was constructed through epoxide ring opening of an optically active β,γ-epoxy aldehyde, synthesized in situ by Swern oxidation of the corresponding alcohol. Finally, the target compounds were synthesized through a Sonogashira coupling of a C9–C22 iodide and methyl (Z)-oct-4-en-7-ynoate or methyl oct-7-ynoate, respectively.
Opportunities Using Boron to Direct Reactivity in the Organic Solid State
Authors: MacGillivray Leonard R. , Campillo-Alvarado Gonzalo 
Published: November 2020

AbstractThis Account describes work by our research group that highlights opportunities to utilize organoboron molecules to direct chemical reactivity in the organic solid state. Specifically, we convey a previously unexplored use of hydrogen bonding of boronic acids and boron coordination in boronic esters to achieve [2+2]-photocycloadditions in crystalline solids. Organoboron molecules act as templates or ‘shepherds’ to organize alkenes in a suitable geometry to undergo regio- and stereoselective [2+2]-photocycloadditions in quantitative yields. We also provide a selection of publications that served as an inspiration for our strategies and offer challenges and opportunities for future developments of boron in the field of materials and solid-state chemistry.1	Introduction1.1	Template Strategy for [2+2]-Photocycloadditions in the Solid State2	Boronic Acids as Templates for [2+2]-Photocycloadditions in the Solid State2.1	Supramolecular Catalysis of [2+2]-Photocycloadditions in the Solid State Using Boronic Acids3	Boronic Esters as Templates for [2+2]-Photocycloadditions in the Solid State3.1	Application of Photoproducts: Separation of Thiophene from Benzene through Crystallization3.2	Crystal Reactivity of B←N-Bonded Adducts: The Case of Styryl­thiophenes4	Conclusions and Perspectives