bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and implementation of a nurse-led allergy clinic model in primary care: feasibility trial protocol
Authors: Kelman Margaret , Hammersley Victoria , Kendall Marilyn , Mukherjee Mome , Morrice Lynn , Harley Susan , Schwarze Jürgen , Sheikh Aziz 
Published: December 2019

AbstractIn the United Kingdom, there are acknowledged short comings in allergy care provision for patients seen in primary care. There is a lack of allergy training for healthcare professionals and this leads to inappropriate referrals to the limited number of allergy specialists. The primary aims of this study are to assess the feasibility of delivering and evaluating a new nurse-led allergy service in primary care, measured by recruitment, retention and quality of life. This is a single arm feasibility trial in which up to 250 participants referred to the nurse-led allergy clinic will receive the intervention and complete 6–12 weeks follow-up before being referred back to their usual care. Primary outcomes for this study will be establishment of clinics, recruitment and retention rates, and estimates of change in disease-specific quality of life measures. Secondary outcomes will be acceptability of the new service to participants/carers and healthcare professionals. A sample of participants and professional stakeholders will take part in more in-depth semi-structured qualitative interviews. Data from this feasibility trial will be used to inform plans for a pilot randomised controlled trial of nurse-led allergy clinics.
A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples
Authors: deWaard Jeremy R. , Ratnasingham Sujeevan , Zakharov Evgeny V. , Borisenko Alex V. , Steinke Dirk , Telfer Angela C. , Perez Kate H. J. , Sones Jayme E. , Young Monica R. , Levesque-Beaudin Valerie , Sobel Crystal N. , Abrahamyan Arusyak , Bessonov Kyrylo , Blagoev Gergin , deWaard Stephanie L. , Ho Chris , Ivanova Natalia V. , Layton Kara K. S. , Lu Liuqiong , Manjunath Ramya , McKeown Jaclyn T. A. , Milton Megan A. , Miskie Renee , Monkhouse Norm , Naik Suresh , Nikolova Nadya , Pentinsaari Mikko , Prosser Sean W. J. , Radulovici Adriana E. , Steinke Claudia , Warne Connor P. , Hebert Paul D. N. 
Published: December 2019

AbstractThe reliable taxonomic identification of organisms through DNA sequence data requires a well parameterized library of curated reference sequences. However, it is estimated that just 15% of described animal species are represented in public sequence repositories. To begin to address this deficiency, we provide DNA barcodes for 1,500,003 animal specimens collected from 23 terrestrial and aquatic ecozones at sites across Canada, a nation that comprises 7% of the planet’s land surface. In total, 14 phyla, 43 classes, 163 orders, 1123 families, 6186 genera, and 64,264 Barcode Index Numbers (BINs; a proxy for species) are represented. Species-level taxonomy was available for 38% of the specimens, but higher proportions were assigned to a genus (69.5%) and a family (99.9%). Voucher specimens and DNA extracts are archived at the Centre for Biodiversity Genomics where they are available for further research. The corresponding sequence and taxonomic data can be accessed through the Barcode of Life Data System, GenBank, the Global Biodiversity Information Facility, and the Global Genome Biodiversity Network Data Portal.
Hybrid Organic/Inorganic Nano-I-Beam for Structural Nano-mechanics
Authors: Elmoselhy Salah A. M. 
Published: December 2019

AbstractFor years Carbon nano-tube has shown merits in industrial applications including high structural strength-to-weight ratio. However, from structural mechanics perspective the tube geometrical cross-section is less favored for providing high structural stiffness and strength. Hybrid Organic/Inorganic Nano-I-Beam is thus introduced for improved Structural Nano-mechanics. It has been found that both Wide Flange Nano-I-Beam and Equal Flange & Web Nano-I-beam provide higher structural stiffness and less induced stress and thus longer service life than Nano-Tube. It has been also found that Wide Flange Nano-I-Beam provides higher structural stiffness and less induced stress and thus longer service life than Equal Flange & Web Nano-I-beam. A thermodynamic model of the growth of nano-tubes accounting for vibrational entropy is presented. The results have cost-effectively potential benefit in applications such as nano-heat engines & sensors.
Lithium alters expression of RNAs in a type-specific manner in differentiated human neuroblastoma neuronal cultures, including specific genes involved in Alzheimer’s disease
Authors: Maloney Bryan , Balaraman Yokesh , Liu Yunlong , Chopra Nipun , Edenberg Howard J. , Kelsoe John , Nurnberger John I. , Lahiri Debomoy K. 
Published: December 2019

AbstractLithium (Li) is a medication long-used to treat bipolar disorder. It is currently under investigation for multiple nervous system disorders, including Alzheimer’s disease (AD). While perturbation of RNA levels by Li has been previously reported, its effects on the whole transcriptome has been given little attention. We, therefore, sought to determine comprehensive effects of Li treatment on RNA levels. We cultured and differentiated human neuroblastoma (SK-N-SH) cells to neuronal cells with all-transretinoic acid (ATRA). We exposed cultures for one week to lithium chloride or distilled water, extracted total RNA, depleted ribosomal RNA and performed whole-transcriptome RT-sequencing. We analyzed results by RNA length and type. We further analyzed expression and protein interaction networks between selected Li-altered protein-coding RNAs and common AD-associated gene products. Lithium changed expression of RNAs in both non-specific (inverse to sequence length) and specific (according to RNA type) fashions. The non-coding small nucleolar RNAs (snoRNAs) were subject to the greatest length-adjusted Li influence. When RNA length effects were taken into account, microRNAs as a group were significantly less likely to have had levels altered by Li treatment. Notably, several Li-influenced protein-coding RNAs were co-expressed or produced proteins that interacted with several common AD-associated genes and proteins. Lithium’s modification of RNA levels depends on both RNA length and type. Li activity on snoRNA levels may pertain to bipolar disorders while Li modification of protein coding RNAs may be relevant to AD.
Deterministic networks for probabilistic computing
Authors: Jordan Jakob , Petrovici Mihai A. , Breitwieser Oliver , Schemmel Johannes , Meier Karlheinz , Diesmann Markus , Tetzlaff Tom 
Published: December 2019

AbstractNeuronal network models of high-level brain functions such as memory recall and reasoning often rely on the presence of some form of noise. The majority of these models assumes that each neuron in the functional network is equipped with its own private source of randomness, often in the form of uncorrelated external noise. In vivo, synaptic background input has been suggested to serve as the main source of noise in biological neuronal networks. However, the finiteness of the number of such noise sources constitutes a challenge to this idea. Here, we show that shared-noise correlations resulting from a finite number of independent noise sources can substantially impair the performance of stochastic network models. We demonstrate that this problem is naturally overcome by replacing the ensemble of independent noise sources by a deterministic recurrent neuronal network. By virtue of inhibitory feedback, such networks can generate small residual spatial correlations in their activity which, counter to intuition, suppress the detrimental effect of shared input. We exploit this mechanism to show that a single recurrent network of a few hundred neurons can serve as a natural noise source for a large ensemble of functional networks performing probabilistic computations, each comprising thousands of units.
DeepSynth: Three-dimensional nuclear segmentation of biological images using neural networks trained with synthetic data
Authors: Dunn Kenneth W. , Fu Chichen , Ho David Joon , Lee Soonam , Han Shuo , Salama Paul , Delp Edward J. 
Published: December 2019

AbstractThe scale of biological microscopy has increased dramatically over the past ten years, with the development of new modalities supporting collection of high-resolution fluorescence image volumes spanning hundreds of microns if not millimeters. The size and complexity of these volumes is such that quantitative analysis requires automated methods of image processing to identify and characterize individual cells. For many workflows, this process starts with segmentation of nuclei that, due to their ubiquity, ease-of-labeling and relatively simple structure, make them appealing targets for automated detection of individual cells. However, in the context of large, three-dimensional image volumes, nuclei present many challenges to automated segmentation, such that conventional approaches are seldom effective and/or robust. Techniques based upon deep-learning have shown great promise, but enthusiasm for applying these techniques is tempered by the need to generate training data, an arduous task, particularly in three dimensions. Here we present results of a new technique of nuclear segmentation using neural networks trained on synthetic data. Comparisons with results obtained using commonly-used image processing packages demonstrate that DeepSynth provides the superior results associated with deep-learning techniques without the need for manual annotation.
Fire and brief human occupations in Iberia during MIS 4: Evidence from Abric del Pastor (Alcoy, Spain)
Authors: Mallol Carolina , Hernández Cristo , Mercier Norbert , Falguères Christophe , Carrancho Ángel , Cabanes Dan , Vidal-Matutano Paloma , Connolly Rory , Pérez Leopoldo , Mayor Alejandro , Ben Arous Eslem , Galván Bertila 
Published: December 2019

AbstractThere is a relatively low amount of Middle Paleolithic sites in Europe dating to MIS 4. Of the few that exist, several of them lack evidence for anthropogenic fire, raising the question of how this period of global cooling may have affected the Neanderthal population. The Iberian Peninsula is a key area to explore this issue, as it has been considered as a glacial refugium during critical periods of the Neanderthal timeline and might therefore yield archaeological contexts in which we can explore possible changes in the behaviour and settlement patterns of Neanderthal groups during MIS 4. Here we report recent data from Abric del Pastor, a small rock shelter in Alcoy (Alicante, Spain) with a stratified deposit containing Middle Palaeolithic remains. We present absolute dates that frame the sequence within MIS 4 and multi-proxy geoarchaeological evidence of in situ anthropogenic fire, including microscopic evidence of in situ combustion residues and thermally altered sediment. We also present archaeostratigraphic evidence of recurrent, functionally diverse, brief human occupation of the rock shelter. Our results suggest that Neanderthals occupied the Central Mediterranean coast of the Iberian Peninsula during MIS 4, that these Neanderthals were not undergoing climatic stress and they were habitual fire users.
Randomised trial on clinical performances and biocompatibility of four high-flux hemodialyzers in two mode treatments: hemodialysis vs post dilution hemodiafiltration
Authors: Morena Marion , Creput Caroline , Bouzernidj Mouloud , Rodriguez Annie , Chalabi Lotfi , Seigneuric Bruno , Lauret Céline , Bargnoux Anne-Sophie , Dupuy Anne-Marie , Cristol Jean-Paul 
Published: December 2019

AbstractThis prospective multicenter randomized comparative cross-over trial aimed at evaluating the influence of hemodialysis vs post-dilution hemodiafiltration with high-flux dialyzers in solute clearance and biocompatibility profile. 32 patients were sequentially dialyzed with Leoceed-21HX, Polypure-22S+, Rexsys-27H and VIE-21A. Primary outcome was β2-microglobulin removal. Secondary outcomes were (i) extraction of other uremic solutes (ii) parameters of inflammation and nutrition and (iii) comparative quantification of perdialytic albumin losses (using total ‘TDC’ vs partial ‘PDC’ collection of dialysate). Significant increases in removal rates of β2-microglobulin (84.7 ± 0.8 vs 71.6 ± 0.8 mg/L), myoglobin (65.9 ± 1.3 vs 38.6 ± 1.3 µg/L), free immunoglobulin light chains Kappa (74.9 ± 0.8 vs 55.6 ± 0.8 mg/L), β-trace protein (54.8 ± 1.3 vs 26.8 ± 1.4 mg/L) and orosomucoid (11.0 ± 1.1 vs 6.0 ± 1.1 g/L) but not myostatin (14.8 ± 1.5 vs 13.0 ± 1.5 ng/mL) were observed in HDF compared to HD when pooling all dialyzers. Rexsys and VIE-A use in both HD and HDF subgroups was associated to a better removal of middle/large-size molecules compared to Leoceed and Polypure, except β2-microglobulin for Rexsys. Inflammatory parameters were unchanged between dialyzers without any interaction with dialysis modality. Mean dialysate albumin loss was comparable between TDC and PDC (1.855 vs 1.826 g/session for TDC and PDC respectively). In addition, a significant difference in albumin loss was observed between dialyzers with the highest value (4.5 g/session) observed using Rexsys. Use of all dialyzers was associated with good removals of the large spectrum of uremic toxins tested and good biocompatibility profiles, with an additional gain in removal performances with HDF. Larger surface area, thinner wall and resultant very high ultrafiltration coefficient of Rexsys should be taken into account in its clear performance advantages.
Determinants of Brain Rhythm Burst Statistics
Authors: Powanwe Arthur S. , Longtin André 
Published: December 2019

AbstractBrain rhythms recorded in vivo, such as gamma oscillations, are notoriously variable both in amplitude and frequency. They are characterized by transient epochs of higher amplitude known as bursts. It has been suggested that, despite their short-life and random occurrence, bursts in gamma and other rhythms can efficiently contribute to working memory or communication tasks. Abnormalities in bursts have also been associated with e.g. motor and psychiatric disorders. It is thus crucial to understand how single cell and connectivity parameters influence burst statistics and the corresponding brain states. To address this problem, we consider a generic stochastic recurrent network of Pyramidal Interneuron Network Gamma (PING) type. Using the stochastic averaging method, we derive dynamics for the phase and envelope of the amplitude process, and find that they depend on only two meta-parameters that combine all the model parameters. This allows us to identify an optimal parameter regime of healthy variability with similar statistics to those seen in vivo; in this regime, oscillations and bursts are supported by synaptic noise. The probability density for the rhythm’s envelope as well as the mean burst duration are then derived using first passage time analysis. Our analysis enables us to link burst attributes, such as duration and frequency content, to system parameters. Our general approach can be extended to different frequency bands, network topologies and extra populations. It provides the much needed insight into the biophysical determinants of rhythm burst statistics, and into what needs to be changed to correct rhythms with pathological statistics.
Epidermal biopolysaccharides from plant seeds enable biodegradable turbulent drag reduction
Authors: Rajappan Anoop , McKinley Gareth H. 
Published: December 2019

AbstractThe high cost of synthetic polymers has been a key impediment limiting the widespread adoption of polymer drag reduction techniques in large-scale engineering applications, such as marine drag reduction. To address consumable cost constraints, we investigate the use of high molar mass biopolysaccharides, present in the mucilaginous epidermis of plant seeds, as inexpensive drag reducers in large Reynolds number turbulent flows. Specifically, we study the aqueous mucilage extracted from flax seeds (Linum usitatissimum) and compare its drag reduction efficacy to that of poly(ethylene oxide) or PEO, a common synthetic polymer widely used as a drag reducing agent in aqueous flows. Macromolecular and rheological characterisation confirm the presence of high molar mass (≥2 MDa) polysaccharides in the extracted mucilage, with an acidic fraction comprising negatively charged chains. Frictional drag measurements, performed inside a bespoke Taylor-Couette apparatus, show that the as-extracted mucilage has comparable drag reduction performance under turbulent flow conditions as aqueous PEO solutions, while concurrently offering advantages in terms of raw material cost, availability, and bio-compatibility. Our results indicate that plant-sourced mucilage can potentially serve as a cost-effective and eco-friendly substitute for synthetic drag reducing polymers in large scale turbulent flow applications.
Nanoparticle-Mediated Cavitation via CO2 Laser Impacting on Water: Concentration Effect, Temperature Visualization, and Core-Shell Structures
Authors: Hu Man , Wang Feng , Huo Peng , Pan Xueqin , Johnson Steven G. , Fink Yoel , Deng Daosheng 
Published: December 2019

AbstractBy taking advantage of seeded polymer nanoparticles and strong photo energy absorption, we report CO2 laser impacting on water to produce cavitation at the air/water interface. Using a high-speed camera, three regimes (no cavitation, cavitation, and pseudo-cavitation) are identified within a broad range of nanoparticles concentration and size. The underlying correlation among cavitation, nanoparticles and temperature is revealed by the direct observation of spatiotemporal evolution of temperature using a thermal cameral. These findings indicate that nanoparticles not only act as preexisted nuclei to promote nucleation for cavitation, but also likely affect temperature to change the nucleation rate as well. Moreover, by exploiting a compound hexane/water interface, a novel core-shell cavitation is demonstrated. This approach might be utilized to attain and control cavitations by choosing nanoparticles and designing interfaces while operating at a lower laser intensity, for versatile technological applications in material science and medical surgery.
Cross-Modal Coordination of Face-Directed Gaze and Emotional Speech Production in School-Aged Children and Adolescents with ASD
Authors: Sorensen Tanner , Zane Emily , Feng Tiantian , Narayanan Shrikanth , Grossman Ruth 
Published: December 2019

AbstractAutism spectrum disorder involves persistent difficulties in social communication. Although these difficulties affect both verbal and nonverbal communication, there are no quantitative behavioral studies to date investigating the cross-modal coordination of verbal and nonverbal communication in autism. The objective of the present study was to characterize the dynamic relation between speech production and facial expression in children with autism and to establish how face-directed gaze modulates this cross-modal coordination. In a dynamic mimicry task, experiment participants watched and repeated neutral and emotional spoken sentences with accompanying facial expressions. Analysis of audio and motion capture data quantified cross-modal coordination between simultaneous speech production and facial expression. Whereas neurotypical children produced emotional sentences with strong cross-modal coordination and produced neutral sentences with weak cross-modal coordination, autistic children produced similar levels of cross-modal coordination for both neutral and emotional sentences. An eyetracking analysis revealed that cross-modal coordination of speech production and facial expression was greater when the neurotypical child spent more time looking at the face, but weaker when the autistic child spent more time looking at the face. In sum, social communication difficulties in autism spectrum disorder may involve deficits in cross-modal coordination. This finding may inform how autistic individuals are perceived in their daily conversations.
3D Smith charts scattering parameters frequency-dependent orientation analysis and complex-scalar multi-parameter characterization applied to Peano reconfigurable vanadium dioxide inductors
Authors: Muller Andrei A. , Moldoveanu Alin , Asavei Victor , Khadar Riyaz A. , Sanabria-Codesal Esther , Krammer Anna , Fernandez-Bolaños Montserrat , Cavalieri Matteo , Zhang Junrui , Casu Emanuele , Schuler Andreas , Ionescu Adrian M. 
Published: December 2019

AbstractRecently, the field of Metal-Insulator-Transition (MIT) materials has emerged as an unconventional solution for novel energy efficient electronic functions, such as steep slope subthermionic switches, neuromorphic hardware, reconfigurable radiofrequency functions, new types of sensors, terahertz and optoelectronic devices. Employing radiofrequency (RF) electronic circuits with a MIT material like vanadium Dioxide, VO2, requires appropriate characterization tools and fabrication processes. In this work, we develop and use 3D Smith charts for devices and circuits having complex frequency dependences, like the ones resulting using MIT materials. The novel foundation of a 3D Smith chart involves here the geometrical fundamental notions of oriented curvature and variable homothety in order to clarify first theoretical inconsistencies in Foster and Non Foster circuits, where the driving point impedances exhibit mixed clockwise and counter-clockwise frequency dependent (oriented) paths on the Smith chart as frequency increases. We show here the unique visualization capability of a 3D Smith chart, which allows to quantify orientation over variable frequency. The new 3D Smith chart is applied as a joint complex-scalar 3D multi-parameter modelling and characterization environment for reconfigurable RF design exploiting Metal-Insulator-Transition (MIT) materials. We report fabricated inductors with record quality factors using VO2 phase transition to program multiple tuning states, operating in the range 4 GHz to 10 GHz.
Formaldehyde quantification using ampicillin is not selective
Authors: Reinbold Raphael , John Tobias , Spingardi Paolo , Kawamura Akane , Thompson Amber L. , Schofield Christopher J. , Hopkinson Richard J. 
Published: December 2019

AbstractFormaldehyde (HCHO) is a simple and highly reactive human metabolite but its biochemistry is poorly defined. A limiting factor in HCHO research is lack of validated quantification methods for HCHO relevant to biological samples. We describe spectroscopic studies on a reported fluorescence-based HCHO detection method involving its reaction with ampicillin. The results validate the structure and fluorescence properties of the HCHO-ampicillin reaction product. However, the same adduct is observed after reaction of ampicillin with glyoxylate. Related fluorophores were formed with other biologically relevant carbonyl compounds. Overall, our studies suggest the ampicillin method is not reliable for selective detection and quantification of HCHO in biological samples.