bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic and metabolomic analysis provides insights into anthocyanin and procyanidin accumulation in pear
Authors: Zhang Zhen , Tian Changping , Zhang Ya , Li Chenzhiyu , Li Xi , Yu Qiang , Wang Shuo , Wang Xinyu , Chen Xuesen , Feng Shouqian 
Published: March 2020

Abstract
Background Pear is one of the most important fruit crops worldwide. Anthocyanins and procyanidins (PAs) are important secondary metabolites that affect the appearance and nutritive quality of pear. However, few studies have focused on the molecular mechanism underlying anthocyanin and PA accumulation in pear . Results We conducted metabolome and transcriptome analyses to identify candidate genes involved in anthocyanin and PA accumulation in young fruits of the pear cultivar ‘Clapp Favorite’ (CF) and its red mutation cultivar ‘Red Clapp Favorite’ ( RCF ). Gene–metabolite correlation analyses revealed a ‘core set’ of 20 genes that were strongly correlated with 10 anthocyanin and seven PA metabolites. Of these, PcGSTF12 was confirmed to be involved in anthocyanin and PA accumulation by complementation of the tt19-7 Arabidopsis mutant. Interestingly, PcGSTF12 was found to be responsible for the accumulation of procyanidin A3, but not petunidin 3, 5-diglucoside, opposite to the function of AtGSTs in Arabidopsis . Transformation with PcGSTF12 greatly promoted or repressed genes involved in anthocyanin and PA biosynthesis, regulation, and transport. Electrophoretic mobility shift and luciferase reporter assays confirmed positive regulation of PcGSTF12 by PcMYB114 . Conclusion These findings identify a core set of genes for anthocyanin and PA accumulation in pear. Of these, PcGSTF12 , was confirmed to be involved in anthocyanin and PA accumulation. Our results also identified an important anthocyanin and PA regulation node comprising two core genes, PcGSTF12 and PcMYB114 . These results provide novel insights into anthocyanin and PA accumulation in pear and represent a valuable data set to guide future functional studies and pear breeding.
Social, economic and family factors associated with binge drinking in Spanish adolescents
Authors: Vargas-Martínez Ana Magdalena , Trapero-Bertran Marta , Mora Toni , Lima-Serrano Marta 
Published: March 2020

Abstract
Background: The main aim of this study was to determine the socioeconomic and family factors associated with binge drinking (BD) in Spanish adolescents who participated in a web-based computer intervention for the prevention of binge drinking known as Alerta Alcohol .
Methods: Longitudinal analyses were carried out in a sample of Andalusian adolescents aged 15 to 19 enrolled in public schools, which was part of a two-arm cluster randomized controlled trial with an intervention group (IG) who received the Alerta Alcohol programme and a control group (CG) who did not receive any active intervention. Panel count data and the following econometric procedures were used: negative binomial, a two-part model and a finite mixture model. The endogenous variable in all models was the number of BD occasions in the last 30 days. A total of 1,247 subjects in the pre-intervention period, with an average age of 16.8 years, plus 612 adolescents in the follow-up period (four months later), were included in the analysis.
Results: In relation to findings, being older ( ≥ 17 years old), having more pocket money and higher family alcohol consumption were associated with greater BD. By contrast, subjects who completed the questionnaire on Wednesday, Thursday or Friday, further from the previous weekend, indicated a lower number of BD occasions.
Conclusions: Our results suggest the need to include families, especially parents and siblings, in interventions aimed at preventing alcohol use among adolescents, given the association shown between BD and both family alcohol consumption and weekly pocket money or availability of money to adolescents. Given the findings with regard to age, future research aimed at intervening in early adolescence to prevent BD would be justified.
Adjunctive therapies for the nonsurgical treatment of peri-implant diseases: systemic review and meta-analysis
Authors: xiao yuhan , Qin Yichun , Yu Haiyang 
Published: March 2020

Abstract
Background Peri-implant diseases are caused by biofilms around the implant and may lead to implant failure. Non-surgical mechanical debridement (MD) with different adjunctive therapies has been applied in the treatment of peri-implant diseases. This systematic review aimed to deduce the optimal adjunctive therapy.Methods Two independent authors screened the literature using MEDLINE and Cochrane Library. Only clinical randomized controlled trials (RCTs) about adjunctive therapies for non-surgical treatment of peri-implant diseases were included in this review. Studies selected were published before February 2020. The clinical outcomes were compared in this meta-analysis.Results: A total of 31 RCTs met the inclusion criteria. The following adjunctive interventions were compared in the included studies: modification of the prosthesis; air abrasive; Er:YAG laser; diode laser; photodynamic therapy; local antibiotics; system antibiotics; probiotics; and enamel matrix derivative. Follow-up ranged from 3 months to 1 year. A statistically significant difference was observed between MD with photodynamic therapy and MD alone at 3 months follow-up ( P < 0.01). However, such a difference was not detected between MD with chlorhexidine and MD alone at 3 months follow-up ( P = 0.61), between MD with probiotics and MD alone ( P = 0.47), and between systemic antibiotics and MD alone ( P = 0.96).Conclusion Currently, the optimal non-surgical intervention is not known. Also, among the interventions with similar efficiency, that with fewer side effects, easy to use, and cost-effective is yet to be identified. Thus, well-designed RCTs with prolonged follow-ups to assess the accurate effectiveness of therapies are imperative.
Mentoring in the clinical training of midwifery students. A focus study of the experiences and opinions of midwifery students at the Medical University of Warsaw participating in a mentoring programme
Authors: Stefaniak Małgorzata , Dmoch-Gajzlerska Ewa 
Published: March 2020

Abstract
Background: The current system of clinical training for midwifery students in Poland is in need of considerable revision to adapt it to the global standards and the expectations of healthcare providers, healthcare users and student midwives themselves. Aim of this study was report the experiences of midwifery students participating in a mentor-led clinical training programme and their opinions of mentoring as a novel training method.Methods: A qualitative descriptive study that used a focus group was undertaken in the period from October 2017 to June 2019. The participants were 12 second- and third-year midwifery students at the Medical University of Warsaw who at various times during the study period had their clinical training in the Department of Obstetrics, Solec Hospital in Warsaw. All students had previous experience of clinical training other than clinical mentorship. At the end of the study, a focus group interview was conducted with all 12 participants. Five questions were selected to guide the focus group discussion: Did you get any valuable learning experience during your clinical training? How did this clinical training differ from your previous clinical training? What was your experience of one-on-one mentoring? Did the mentoring programme meet your expectations? What do you think could be changed to make the proposed mentor-led clinical training more effective? Results: Four themes were identified. The study demonstrated that mentoring was perceived by the participants as an innovative and effective method of clinical training for midwifery students. All students positively evaluated the quality of the mentor-led clinical training which allowed improving their clinical skills and building new competencies. Students believed they could effectively use their clinical skills and make informed decisions in a safe and supportive clinical learning environment. They felt that their inclusion in the therapeutic team contributed to better patient care.Conclusions: The use of innovative forms of clinical training at undergraduate level improves its effectiveness and in the future should be reflected in a high-quality maternity care. Mentoring has its advantages for both, mentor and mentee, but the main goal is to develop and improve professional competencies of the junior partner.
Evaluation of the survival of patients with cirrhosis of viral etiology B / C in 3 hospitals of Kinshasa in the Democratic Republic of Congo
Authors: Nkodila Aliocha , Mbendi Charles , Malumba Vanessa , Tukadila Hervé Alex , Kisalambote Grace , Kumbu Clovis , Tshiamala Pascal , Tshimpi Antoine , Mbendi Sébastien , Ennaji Moustapha Moulay , Longo Benjamin 
Published: March 2020

Abstract
Background and objective : The causes of declining survival in cirrhotic patients are dominated by complications in developing countries. But data on survival among cirrhotics in Sub-Saharan Africa are sketchy. The general objective was to assess the survival and predictors of mortality in patients with viral cirrhosis of the liver in 3 hospitals in the Democratic Republic of Congo. Methods: A historic cohort study from 2009 to December 2018. Patients were recruited and followed up at the Ngaliema Clinic, CUK and HPGRK for 10 years. Death was the endpoint and patients lost to follow-up were censored. The Kaplan Méier curves made it possible to describe the survival of children at the threshold of p <0.05. Results : One hundred and four, 124 were male (75.6%), mean age 50.1 ± 16.1 years, 54.9% HBV viral etiology and 18.9% HBV-HCV co-infection were included. 37.8% cirrhotic had died. The probability of survival for cirrhotic patients at the start of follow-up was 81.7%, 69.5%, 62.2%, 62.2% and 62.2%, respectively, at 1 year, 2 years, 3 years, 4 years and 10 years. The predictors of mortality were age ≥60 years, hepatocellular carcinoma, encephalopathy, Child Pugh class B and C and low Hb. Conclusion: complications in cirrhotics are the factors of poor prognosis for the survival of patients in this cohort. Early detection of cirrhosis increases the survival of cirrhotic patients.
Depressive Symptoms And Stigmatization Among Parents Of Children With Intellectual Disabilities: Cross-Sectional Survey In Public Primary Special Schools In Nairobi, Kenya
Authors: Githara Mercy , Obondo Anne , Tele Albert , Thornicroft Graham , Kumar Manasi 
Published: March 2020

Abstract
Background Parents of children with intellectual disabilities may develop depressive symptoms and are prone to stigmatization due to stressful challenges encountered when providing care for their children. The study objective was to determine the prevalence of depressive symptoms and stigmatization among parents of children with intellectual disabilities. Method A cross-sectional descriptive study design was used to sample 121 parents of children with intellectual disabilities in four public primary special schools in Nairobi, Kenya Data were collected using a researcher designed socio-demographic questionnaire, Beck Depression Inventory (BDI-II) to screen depressive symptoms and the DISC-12 used to determine stigmatization among parents of children with intellectual disabilities. Descriptive statistics were used to examine the depression scores, discrimination and stigma scores. Independent samples t-test, and one-way analyses of variance (ANOVA) were used to identify group differences at the bivariate level. Generalized linear models were used to identify independent predictors of discrimination and Stigma. Results Of the 121 parents assessed for depression 24% (n=29) of parents met the criterion for being at risk of depression using BDI scores (score of 21 and above). Stigma and discrimination were significantly related to parent’s depressive symptoms, independently of other variables. On multivariate linear regression individual predictors showed that being female (P=<0.001), younger age of between 18-26 (P=<0.003), being divorced/ separated (p=<0.0001), lower education (P=<0.0001) and caregiver burden (P=0.033) were predictors of depression and high levels of stigmatization. Conclusions The risk of depression is high among parents of children with intellectual disabilities. Results of this study suggest that interventions could be developed that have a focus on the health or well- being of parents. Reduction of societal stigma could reduce psychological distress to parents.
Comparison between buttress plate and anteroposterior screw internal fixation for posterior pilon fracture: a retrospective study
Authors: zhang linyuan , liu zhiqing , wang yueting , yu chao , tang jian , sun yuehua , zhu zhen'an , xu chen , liu fengxiang 
Published: March 2020

Abstract
Background Posterior pilon fracture (PPF) is a serious ankle injury caused by combined vertical and rotational trauma. Surgical treatment includes buttress plate (BP), anteroposterior screw (APS) and posteroanterior screw (PAS) fixation. This study aimed to compare the therapeutic effects after PPF internal fixation with BPs and APSs. Methods From January 2015 to June 2018, 37 patients with PPFs underwent surgical treatment in our institution. The patients were divided into the BP (11 patients) and APS (26 patients) groups according to the internal fixation method. Bone healing time and postoperative complications were recorded. At the last follow-up, American Orthopedic Foot and Ankle Society (AOFAS) scores, visual analog scale (VAS) scores and Burwell-Charnley scores were used to evaluate ankle joint function, ankle joint pain, and fracture reduction on imaging, respectively. Results No significant difference in bone healing time was found between the two groups ( P > 0.05). Local necrosis of the posterolateral incision and chronic ankle pain occurred in 1 case each in the BP group. Chronic ankle joint pain occurred in 4 cases in the APS group. Two cases of soft tissue irritation were caused by screws that were too long. Local numbness of the posterolateral incision occurred in 1 case. At the last follow-up, no significant difference in VAS scores between the two groups at rest ( P = 0.477), but a significant difference during exercise was noted ( P = 0.033). AOFAS scores were better in the BP group than those in the APS group ( P = 0.002). According to the Burwell-Charnley scoring system, 11 cases were anatomically reduced in the BP group. In the APS group, anatomical reduction was achieved in 19 cases and fair in 7 cases. Conclusion Compared with APSs, BPs resulted in better reduction, ankle joint functional recovery and complication rates. BPs are recommended for internal fixation of PPFs.
Human epididymis protein 4 is a new biomarker to predict the prognosis of progressive fibrosing interstitial lung disease
Authors: Nishiyama Naoki , Masuo Masahiro , Nukui Yoshihisa , Tateishi Tomoya , Kishino Mitsuhiro , Tateishi Ukihide , Morota Kaori , Ohbo Kazuyuki , Miyazaki Yasunari 
Published: March 2020

Abstract
BACKGROUND The clinical course and prognosis of progressive fibrosing interstitial lung diseases ( PF-ILDs) vary from individual to individual. Predictive serum biomarkers for the management of the disease are needed. Serum human epididymis protein 4 (HE4) has been reported to be elevated in patients with IPF, yet its clinical utility has not been elucidated. We evaluated whether serum HE4 could be a biomarker for patients with PF-ILD.METHODS Serum HE4 was measured in a retrospective study that consisted of 34 patients with PF-ILD and 40 healthy volunteers. The relationship between serum HE4 levels and clinical parameters or prognosis was investigated. To validate the significance of the results, a prospective observational study that consisted of 37 patients with PF-ILD and 40 control patients without PF-ILD was performed.RESULTS Serum HE4 was higher in patients with PF-ILD than in health volunteers ( P < 0.01). A correlation of the serum HE4 levels with the extent of honeycombing on chest high-resolution computed tomography was identified (r = 0.41, P = 0.015). In multivariate analysis by the Cox proportional hazard model, higher HE4 levels (> 238 pmol/l) were associated with elevated mortality risk (HR 7.27, 95% CI 1.56-34.0, P = 0.01 in the derivation cohort; HR 44.3, 95% CI 4.19-468, P < 0.01 in validation cohort).CONCLUSIONS Serum HE4 levels may serve as a new diagnostic and prognostic biomarker for patients with PF-ILD.
The new nutrition label is a combination of the physical activity, traffic light and warning labels: a qualitative study
Authors: Seyedhamzeh Shirin , Nedjat Saharnaz , Shakibazadeh Elham , Hosseini Hedayat , Doustmohammadian Azam , Motlagh Ahmad-reza Dorosty 
Published: March 2020

Abstract
Background Unhealthy diets and inadequate physical activity are important factors in the increasing trend of obesity. In order to mitigate this harmful trend, an effective policy must be developed to target both unhealthy diets and insufficient physical activity. The implementation of physical activity recommendations in nutrition labeling may influence the consumers’ food choices. In this qualitative study, we designed a new type of physical activity calorie equivalent (PACE) label by considering the viewpoints of household representatives and experts. Method Ten focus group discussions (FGDs) were conducted with 63 mothers from different regions of Tehran based on the participants’ socioeconomic status. Furthermore, ten interviews were conducted with food quality control (FQC) experts to examine the new PACE label. The preliminary designs were based on the mothers’ viewpoints. We then presented these new labels to the nutritionists and food industry experts in two separate FGDs. After taking their opinions into consideration, we applied certain changes to the labels and emailed them to the mothers. Consequently, the final versions of the new labels were designed. Conventional content analysis was applied with the MAXQDA10 software. Result Two main themes were defined: (1) the stakeholders’ perspectives on PACE labeling (2) the new PACE label’s characteristics including the appearance and the nutritional information on the new label. Conclusion The new label is a combination of the physical activity, traffic light and warning labels.
Care taker’s compliance and Associated factors in integrated management of newborn and childhood illness with respect to Counseling in Enebise Sarmidir woreda, West Amhara, Ethiopia.
Authors: Zeru Taye , Alemirew Dehabo 
Published: March 2020

Abstract
Background: - The Integrated Management of Newborn and Childhood Illness strategy encompasses a range of interventions to prevent and manage the major childhood illness, both in health facilities and in the home. Information on actual compliance of care takers in integrated management of newborn and childhood illness is important for program planners, policy makers, health care providers and care takers to design appropriate local and national intervention plan, to increase community participation and to improve knowledge of care takers. Based on our knowledge there are limited studies regarding to care takers compliance towards integrated management of new born and childhood illness in the study area. Therefore the result of this study used for design appropriate intervention.Objective: - The objective of this study was to assess the care takers compliance and associated factors in integrated management of newborn and childhood illness with respect to antibiotic treatment.Methods:-Community based cross-sectional study was conducted. The study populations were all care takers of under-five children who attended integrated management of newborn and childhood illness clinic at health center. A total of 394 respondents were randomly selected by using systematic random sampling method from the seven health centers. Information on the care takers compliance was collected using structured, pre-tested questionnaire. Data entry and analysis were conducting using Statistical Package for Social Science version 16. Significance of the study was presented by using adjust odd ratio, confidence interval and p value. The result was presented in texts, tables and graphs.Result:-Three hundred seventy three care takers with under-five year children were voluntary to be interviewed, making the response rate 94.7 %. The findings of this study showed that 144(39%) were compliance to antibiotic treatment. Compliance to antibiotic treatment was significantly associated with post-secondary education with (AOR=5.33, 95%CI: 2.557, 11.109), private/NGO employee with (AOR=5.936, 95%CI: 1.632, 21.582), knowledge with (AOR=1.806, 95%CI: 1.096, 2.976) and distance with (AOR=2.366, 95%CI: 1.419, 3.945). Conclusion and recommendation: The finding of this study revealed that the high rates of noncompliance with counseling were identified. Therefore, the implementation of integrated management of childhood illness activities at all levels should be strengthened. There is a great need to improve the social status of care takers in order to reduce child hood morbidity and mortality.
WITHDRAWN: Correlation between cardiorespiratory coupling and six-minute walking tests in patients with chronic obstructive pulmonary disease
Authors: Huang Yu-Chen , Lin Ting-Yu , Wu Hau-Tieng , Chang Po-Jui , Lo Chun-Yu , Wang Tsai-Yu , Kuo Chih-Hsi Scott , Lin Shu-Min , Chung Fu-Tsai , Lin Horng-Chyuan , Hsieh Meng-Heng , Lo Yu-Lun 
Published: March 2020

Abstract
Background : Cardiovascular disease is a common comorbidity and cause of mortality among patients with chronic obstructive pulmonary disease (COPD). However, the interaction between the heart and lungs in COPD patients has yet to be fully elucidated.Aim : Our objective in this study was to characterize cardiorespiratory interactions in terms of cardiorespiratory coupling (CRC) using the synchrogram index of the heart rate and respiration flow signals.Methods : This prospective study examined 10 normal subjects and 55 COPD patients. Linear regression and forward stepwise regression were used to determine the correlation between the synchrogram index and the six-minute walking test.Results : K-means clustering analysis was used to separate the 55 COPD patients into a synchronized group (median 0.89 (0.64-0.97), n=43) and a desynchronized group (median 0.23 (0.02-0.51), n=12) based on the synchrogram index. In this study, the synchrogram index was significantly correlated with the six-minute walking distance (r 2 =0.3, sigma T=0.02) and the distance saturation product (r 2 =0.3, sigma T =0.03). Note that age was a significant confounding factor.Conclusion : The synchrogram index shows clinical potential for the stratification of COPD patients for treatment.