bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artesunate induces mitochondria-mediated apoptosis of human retinoblastoma cells by upregulating Kruppel-like factor 6
Authors: Yang Ying , Wu Nandan , Wu Yihui , Chen Haoting , Qiu Jin , Qian Xiaobing , Zeng Jieting , Chiu Kin , Gao Qianying , Zhuang Jing 
Published: November 2019

AbstractRetinoblastoma (RB) is the most common primary intraocular malignancy in children. Intravitreal chemotherapy achieves favorable clinical outcomes in controlling RB vitreous seeds, which are a common reason for treatment failure. Thus, a novel, effective and safe intravitreal chemotherapeutic drug is urgently required. The malaria drug artesunate (ART) recently demonstrated remarkable anticancer effects with mild side effects. The purpose of this study is to investigate the anti-RB efficacy, the underlying mechanism and the intraocular safety of ART. Herein, we verified that ART inhibits RB cell viability and induces cell apoptosis in a dose- and time-dependent manner. Microarray analysis revealed that Kruppel-like factor 6 (KLF6) was upregulated after ART treatment, and this was further confirmed by real-time PCR and western blot assays. Silencing of KLF6 expression significantly reversed ART-induced RB cell growth inhibition and apoptosis. Furthermore, ART activated mitochondria-mediated apoptosis of RB cells, while silencing KLF6 expression significantly inhibited this effect. In murine xenotransplantation models of RB, we further confirmed that ART inhibits RB tumor growth, induces tumor cell apoptosis and upregulates KLF6 expression. In addition, KLF6 silencing attenuates ART-mediated inhibition of tumor growth in vivo. Furthermore, we proved that intravitreal injection of ART in Sprague-Dawley (SD) rats is safe, with no obvious retinal function damage or structural disorders observed by electrophysiology (ERG), fundal photographs, fundus fluorescein angiography (FFA) or optical coherence tomography (OCT) examinations. Collectively, our study revealed that ART induces mitochondrial apoptosis of RB cells via upregulating KLF6, and our results may extend the application of ART to the clinic as an effective and safe intravitreal chemotherapeutic drug to treat RB, especially RB with vitreous seeds.
The Firre locus produces a trans-acting RNA molecule that functions in hematopoiesis
Authors: Lewandowski Jordan P. , Lee James C. , Hwang Taeyoung , Sunwoo Hongjae , Goldstein Jill M. , Groff Abigail F. , Chang Nydia P. , Mallard William , Williams Adam , Henao-Meija Jorge , Flavell Richard A. , Lee Jeannie T. , Gerhardinger Chiara , Wagers Amy J. , Rinn John L. 
Published: November 2019

AbstractRNA has been classically known to play central roles in biology, including maintaining telomeres, protein synthesis, and in sex chromosome compensation. While thousands of long noncoding RNAs (lncRNAs) have been identified, attributing RNA-based roles to lncRNA loci requires assessing whether phenotype(s) could be due to DNA regulatory elements, transcription, or the lncRNA. Here, we use the conserved X chromosome lncRNA locus Firre, as a model to discriminate between DNA- and RNA-mediated effects in vivo. We demonstrate that (i) Firre mutant mice have cell-specific hematopoietic phenotypes, and (ii) upon exposure to lipopolysaccharide, mice overexpressing Firre exhibit increased levels of pro-inflammatory cytokines and impaired survival. (iii) Deletion of Firre does not result in changes in local gene expression, but rather in changes on autosomes that can be rescued by expression of transgenic Firre RNA. Together, our results provide genetic evidence that the Firre locus produces a trans-acting lncRNA that has physiological roles in hematopoiesis.
eIF4A supports an oncogenic translation program in pancreatic ductal adenocarcinoma
Authors: Chan Karina , Robert Francis , Oertlin Christian , Kapeller-Libermann Dana , Avizonis Daina , Gutierrez Johana , Handly-Santana Abram , Doubrovin Mikhail , Park Julia , Schoepfer Christina , Da Silva Brandon , Yao Melissa , Gorton Faith , Shi Junwei , Thomas Craig J. , Brown Lauren E. , Porco John A. , Pollak Michael , Larsson Ola , Pelletier Jerry , Chio Iok In Christine 
Published: November 2019

AbstractPancreatic ductal adenocarcinoma (PDA) is a lethal malignancy with limited treatment options. Although metabolic reprogramming is a hallmark of many cancers, including PDA, previous attempts to target metabolic changes therapeutically have been stymied by drug toxicity and tumour cell plasticity. Here, we show that PDA cells engage an eIF4F-dependent translation program that supports redox and central carbon metabolism. Inhibition of the eIF4F subunit, eIF4A, using the synthetic rocaglate CR-1-31-B (CR-31) reduced the viability of PDA organoids relative to their normal counterparts. In vivo, CR-31 suppresses tumour growth and extends survival of genetically-engineered murine models of PDA. Surprisingly, inhibition of eIF4A also induces glutamine reductive carboxylation. As a consequence, combined targeting of eIF4A and glutaminase activity more effectively inhibits PDA cell growth both in vitro and in vivo. Overall, our work demonstrates the importance of eIF4A in translational control of pancreatic tumour metabolism and as a therapeutic target against PDA.
Optical vector analysis with attometer resolution, 90-dB dynamic range and THz bandwidth
Authors: Qing Ting , Li Shupeng , Tang Zhenzhou , Gao Bindong , Pan Shilong 
Published: November 2019

AbstractOptical vector analysis (OVA) capable of achieving magnitude and phase responses is essential for the fabrication and application of emerging optical devices. Conventional OVA often has to make compromises among resolution, dynamic range, and bandwidth. Here we show an original method to meet the measurement requirements for ultra-wide bandwidth, ultra-high resolution, and ultra-large dynamic range simultaneously, based on an asymmetric optical probe signal generator (ASG) and receiver (ASR). The ASG and ASR remove the measurement errors introduced by the modulation nonlinearity and enable an ultra-large dynamic range. Thanks to the wavelength-independence of the ASG and ASR, the measurement range can increase by 2 N times by applying an N-tone optical frequency comb without complicated operation. In an experiment, OVA with a resolution of 334 Hz (2.67 attometer in the 1550-nm band), a dynamic range of > 90 dB and a measurement range of 1.075 THz is demonstrated.
Pipetting-based immunoassay for point-of-care testing: Application for detection of the influenza A virus
Authors: Noh Ji Yeong , Yoon Sun-Woo , Kim Youngji , Lo Thi Van , Ahn Min-Ju , Jung Min-Chul , Le Tran Bac , Na Woonsung , Song Daesub , Le Van Phan , Haam Seungjoo , Jeong Dae Gwin , Kim Hye Kwon 
Published: November 2019

AbstractPoint-of-care tests (POCT) for pathogens are considered important for low-resource countries and facilities. Although lateral flow immunoassays (LFIA) have many advantages including speed and ease of use, their sensitivity is limited without specific equipment. Furthermore, their response cannot be enhanced through enzymatic reactions. Owing to these limitations, LFIAs have not yet been generally adopted as the standard protocol for in vitro analysis of infectious pathogens. We aimed to develop a novel pipetting-based immunoassay using a removable magnetic ring-coupled pipette tip. The “magnetic bead-capture antibody-targeted protein complex” was simply purified by pipetting and quantified by enzymatic colour development or using a lateral flow system. This pipetting-based immunoassay was applied to detect the nucleoprotein (NP) of the influenza A virus. Using an HRP-conjugated monoclonal antibody as a probe, the assay allowed for specific and sensitive detection. Furthermore, when this assay was applied exclusively for antigen capture in the lateral flow system, the limit of detection improved 100-fold and displayed greater sensitivity than the lateral flow system alone. Therefore, the pipetting-based immunoassay may be potentially used as a sensitive POCT to clinically detect a target antigen.
Early Life Trauma Has Lifelong Consequences for Sleep And Behavior
Authors: Lewin Monica , Lopachin Jenna , Delorme James , Opendak Maya , Sullivan Regina M. , Wilson Donald A. 
Published: November 2019

AbstractSleep quality varies widely across individuals, especially during normal aging, with impaired sleep contributing to deficits in cognition and emotional regulation. Sleep can also be impacted by a variety of adverse events, including childhood adversity. Here we examined how early life adverse events impacted later life sleep structure and physiology using an animal model to test the relationship between early life adversity and sleep quality across the life span. Rat pups were exposed to an Adversity-Scarcity model from postnatal day 8–12, where insufficient bedding for nest building induces maternal maltreatment of pups. Polysomnography and sleep physiology were assessed in weaning, early adult and older adults. Early life adversity induced age-dependent disruptions in sleep and behavior, including lifelong spindle decreases and later life NREM sleep fragmentation. Given the importance of sleep in cognitive and emotional functions, these results highlight an important factor driving variation in sleep, cognition and emotion throughout the lifespan that suggest age-appropriate and trauma informed treatment of sleep problems.
Harmonic light scattering study reveals structured clusters upon the supramolecular aggregation of regioregular poly(3-alkylthiophene)
Authors: Moris Michèle , Van Den Eede Marie-Paule , Koeckelberghs Guy , Deschaume Olivier , Bartic Carmen , Van Cleuvenbergen Stijn , Clays Koen , Verbiest Thierry 
Published: November 2019

AbstractSolubilized poly(3-alkylthiophene)s are known to self-assemble into well-ordered supramolecular aggregates upon lowering the solvent quality. This supramolecular organization largely determines the optical and electronic properties of these polymers. However, despite numerous studies the exact mechanism and kinetics of the aggregation process and the role of external stimuli are still poorly understood. Classical characterization techniques such as electronic spectroscopy, dynamic light scattering, and diffraction-based techniques have not been able to provide a full understanding. Here we use second-harmonic scattering (SHS) and third-harmonic scattering (THS) techniques to investigate this supramolecular aggregation mechanism. Our results indicate that the actual supramolecular aggregation is preceded by the formation of structured polymer-solvent clusters consistent with a nonclassical crystallization pathway.
Niobium pentoxide nanomaterials with distorted structures as efficient acid catalysts
Authors: Skrodczky Kai , Antunes Margarida M. , Han Xianying , Santangelo Saveria , Scholz Gudrun , Valente Anabela A. , Pinna Nicola , Russo Patrícia A. 
Published: November 2019

AbstractNiobium pentoxides are promising acid catalysts for the conversion of biomass into fuels and chemicals. Developing new synthesis routes is essential for designing niobium pentoxide catalysts with improved activity for specific practical processes. Here we show a synthesis approach in acetophenone, which produces nanostructured niobium pentoxides with varying structure and acidity that act as efficient acid catalysts. The oxides have orthorhombic structures with different extents of distortions and coordinatively unsaturated metal atoms. A strong dependence is observed between the type and strength of the acid sites and specific structural motifs. Ultrasmall niobium pentoxide nanoparticles, which have strong Brønsted acidity, as well as Lewis acidity, give product yields of 96% (3 h, 140 °C, 100% conversion), 85% (3 h, 140 °C, 86% conversion), and 100% (3 h, 110 °C, 100% conversion) in the reactions of furfuryl alcohol, 5-(hydroxymethyl)furfural, and α-angelica lactone with ethanol, respectively.
Camptodactyly Caused by an Anomalous Origin of the Flexor Digitorum Superficialis Tendon: A Case Report and Review of the Literature
Authors: Duci Shkelzen B. 
Published: November 2019

AbstractCamptodactyly is a flexion contracture of the proximal interphalangeal joints and is known as an isolated malformation that affects 1 in 300 in the population and can be inherited as an autosomal dominant trait with variable expression.A 17-year-old female was referred to the Clinic of Plastic Surgery, University Clinical Center of Kosovo, Prishtina, for the first time with camptodactyly of the little finger in the right hand. She was presented with a progressive flexion contracture of the proximal interphalangeal joint greater than 110 degrees of her right little finger.According to our observations from outpatient consultations, we concluded that the case of camptodactyly in the little finger in the flexible form (>110 degrees), which underwent surgical treatment, presented excellent result. Therefore, we think that the surgical technique used in our case report will contribute to treating this complicated deformity.
The effect of craniectomy size on mortality, outcome, and complications after decompressive craniectomy at a rural trauma center
Authors: Sedney Cara L. , Julien Terrence , Manon Jacinto , Wilson Alison 
Published: November 2019

ABSTRACT
Introduction: Decompressive craniectomy (DC) has increasing support with current studies suggesting an improvement in both survival rates and outcomes with this intervention. However, questions surround this procedure; specifically, no evidence has indicated the optimal craniectomy size. Larger craniectomy is thought to better decrease intracranial pressure, but with a possible increase in complication rates. Our hypothesis is that a larger craniectomy may improve mortality and outcome, but may increase complication rates. Materials and Methods: A retrospective observational therapeutic study was undertaken to determine if craniectomy size is related to complication rates, mortality, or outcome. Our institution's Trauma Registry was searched for patients undergoing DC. Craniectomy size was measured by antero-posterior (AP) diameter. Mortality, outcome (through admission and discharge Glasgow Coma Score and Glasgow Outcome Scale), and complications (such as re-bleeding, re-operation, hygroma, hydrocephalus, infection, and syndrome of the trephined) were noted. Complications, mortality, and outcome were then compared to craniectomy size, to determine if any relation existed to support our hypothesis. Results: 20 patients met criteria for inclusion in this study. Craniectomy size as measured by AP diameter was correlated with a statistically significant improvement in mortality within the group. All patients with a craniectomy size less than 10 cm died. However, outcome was not significantly related to craniectomy size in the group. Similarly, complication rates did not differ significantly compared to craniectomy size. Discussion: This study provides Level 3 evidence that craniectomy size may be significantly related to improved mortality within our group, supporting our initial hypothesis; however, no significant improvement in outcome was seen. Similarly, in contrast to our hypothesis, complication rates did not significantly correlate with craniectomy size.
Organophosphate intermediate syndrome with neurological complications of extrapyramidal symptoms in clinical practice
Authors: Detweiler Mark B. 
Published: November 2019

ABSTRACTOrganophosphates (OPs) are ubiquitous in the world as domestic and industrial agricultural insecticides. Intentional poisoning as suicides attempts are clinical phenomena seen in emergency departments and clinics in agricultural areas. Intermediate syndrome with the neurological complication of extra pyramidal symptoms following acute OP ingestion may occur in pediatric and adult cases. While death is the most serious consequence of toxic OP doses, low levels of exposure and nonfatal doses may disrupt the neurobehavioral development of fetuses and children in addition to bring linked to testicular cancer and male and female infertility. These are disturbing. Chronic and acute toxicity from OPs are barriers to the health of our present and future generations. Symptoms and treatment of acute and chronic OP exposure are briefly referenced with inclusion of the intermediate syndrome. Suggestions for local and systemic reduction of the acute and long term consequences of OP ingestion are opined.
Multivalued solutions of hyperbolic Monge-Ampère equations: solvability, integrability, approximation
Authors: Tunitsky D. V. 
Published: November 2019

Abstract
Solvability in the class of multivalued solutions is investigated for Cauchy problems for hyperbolic Monge-Ampère equations. A characteristic uniformization is constructed on definite solutions of this problem, using which the existence and uniqueness of a maximal solution is established. It is shown that the characteristics in the different families that lie on a maximal solution and converge to a definite boundary point have infinite lengths. In this way a theory of global solvability is developed for the Cauchy problem for hyperbolic Monge-Ampère equations, which is analogous to the corresponding theory for ordinary differential equations. Using the same methods, a stable explicit difference scheme for approximating multivalued solutions can be constructed and a number of problems which are important for applications can be integrated by quadratures.
Bibliography: 23 titles.
Boundary behaviour of open discrete mappings on Riemannian manifolds. II
Authors: Ilyutko D. P. , Sevost’yanov E. A. 
Published: November 2019

Abstract
The boundary behaviour of classes of ring mappings, which generalize quasiconformal mappings in the sense of Gehring, is under investigation. Theorems proving that they have continuous boundary extensions are established in terms of prime ends of regular domains. Results on the equicontinuity of mappings in these classes in the closure of a fixed domain are also established in these terms.
Bibliography: 45 titles.
Spatial and temporal pulse shaping for lateral and depth resolved two-photon excited fluorescence contrast
Authors: Kussicke A , Tegtmeier M , Büchau F , Heyne K , Lindinger A 
Published: November 2019

Abstract
We report combined temporal and spatial laser pulse shaping to perform lateral and depth dependent two-photon excited fluorescence of dyes. For generating the specific spatially and temporally phase tailored pulses a temporal pulse shaper and a subsequent spatial pulse shaper are employed. Simultaneous spatial and temporal shaping is presented for two-photon excited fluorescence by applying temporal third order phase functions on spatially different light field components. Moreover, the prospects of spatial shaping are demonstrated by applying various lateral two-photon fluorescence pattern. In particular, a depth dependent excitation of different dyes is performed which leads to a high axially resolved fluorescence contrast. The introduced spatial and temporal shaping technique provides new perspectives for biophotonic imaging applications.
Conference key agreement with single-photon interference
Authors: Grasselli Federico , Kampermann Hermann , Bruß Dagmar 
Published: November 2019

Abstract
The intense research activity on Twin-Field (TF) quantum key distribution (QKD) is motivated by the fact that two users can establish a secret key by relying on single-photon interference in an untrusted node. Thanks to this feature, variants of the protocol have been proven to beat the point-to-point private capacity of a lossy quantum channel. Here we generalize the main idea of the TF-QKD protocol introduced by Curty et al to the multipartite scenario, by devising a conference key agreement (CKA) where the users simultaneously distill a secret conference key through single-photon interference. The new CKA is better suited to high-loss scenarios than previous multipartite QKD schemes and it employs for the first time a W-class state as its entanglement resource. We prove the protocol’s security in the finite-key regime and under general attacks. We also compare its performance with the iterative use of bipartite QKD protocols and show that our truly multipartite scheme can be advantageous, depending on the loss and on the state preparation.
The probability determination of the queuing system state of the motor transport enterprise
Authors: Bulatov S V 
Published: November 2019

Abstract
The article describes the queuing system by the example of one-passenger motor transport enterprises for the purpose of carrying out the analysis of the applications flow for spare parts, the conditions identifications of the formation of the waiting list. All this including the determination of the optimum number of the service stations for units and sets will allow serving in due time the rolling stock without idle times.
The synthesis of motion laws for cam mechanisms with additional movement of the follower
Authors: Podgornyj Yu I , Skeeba V Yu , Kirillov A V , Martyushev N V , Borisov M A 
Published: November 2019

Abstract
The research discusses the design of cam mechanisms for special purposes. The analysis of the scientific literature on this issue indicates that at present the synthesis of the cam mechanisms motion laws is carried out mainly using a standard set of acceleration curves or displacements. In some cases, the designer needs to synthesize an acceleration law which should be task-specific and provide for a certain production step. Additional areas on the cam profile which perform specified motions of the follower are very important for the synthesis of such mechanisms. There may be several areas of this type. The relevance of the study is due to the fact that the mechanisms that work using a time-compressed cyclic diagram cannot be modernized on the basis of the existing methods for the synthesis of cam mechanisms; therefore, it is not possible to enhance the performance of such mechanisms without a significant change in the laws of motion.
Determining the operational loads of the hybrid metalworking machines drive
Authors: Skeeba V Yu , Skeeba P Yu 
Published: November 2019

Abstract
Numerous studies are devoted to determining the operational loads of metalworking machines. They employ various approaches including those based on analytical methods of calculation. However, their use is not always appropriate, since they do not take into account many features characteristic of the actual operating conditions of hybrid metalworking machines. The article proposes a universal method of substantiating the performance specification of the integrated machine-tool equipment based on mathematical modeling of its operating conditions. This technique allows generating objective data on the design loads of machine tools, which enables providing the necessary level of reliability of newly designed machines that have no analogues. The proposed approach provides the possibility of using automated design systems at the initial stage of creating the general concept of new process equipment.