bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic, clinical, and therapeutic importance of RANTES‐CCR5 axis in hepatitis A infection: A multiapproach study
Authors: Baruah Vargab , Tiwari Diptika , Hazam Rajib Kishore , Bose Moumita , Bujarbaruah Dipankar , Saikia Anjan Kumar , Kar Premashish , Dutta Sangit , Bose Sujoy 
Published: September 2020

AbstractFulminant hepatic failure (FHF) is a lethal manifestation of hepatitis A virus (HAV) infection, whose underlying mechanisms are poorly understood. We aimed to evaluate the importance of the modulation of the RANTES‐chemokine receptor type 5 (CCR5) signaling axis and its immunomodulatory effects in directing hepatitis A disease pathogenesis using an in silico, in vitro and patient cohort‐based approach. In silico interaction studies were performed using computation approaches with suitable software. Differential expression of relevant cytokines and immune cell markers were studied using real‐time quantitative reverse transcription PCR (qRT‐PCR), enzyme‐linked immunosorbent assay, and flow‐cytometry‐based methods. In the HepG2 cell line, we studied inflammatory responses and susceptibility to HAV infection following RANTES stimulation and antibody blockade of CCR5. The HAV‐VP3 region exhibited high interaction in CCR5: HAV complexes. RANTES levels were significantly increased in FHF cases. Reduced monocyte and T‐cell activation were observed in FHF cases. RANTES expression inversely correlated with viremia but positively correlated with proinflammatory responses. Hyper Th1‐biased immune responses, marked by high interleukin (IL)‐12/IL‐10 ratio were observed in FHF cases, which were also characterized by upregulated tumor necrosis factor‐alpha (TNF‐α) expression and reduced interferon‐gamma expression. In vitro, RANTES was protective against HAV infection but resulted in upregulated TNF‐α expression. Although viral load increased upon the regulation of inflammatory responses by CCR5 blocking, it was still significantly lower compared to control HAV‐infected cells. Our study suggests the importance of RANTES‐CCR5 signaling and linked‐immunomodulation in HAV disease pathogenesis, as well as highlights the utility of CCR5 antagonists as a risk‐reduction strategy in FHF patients. Our findings, therefore, have important implications for the management of high‐risk HAV infections.
Efficiency of Dodecenylsuccinic Amide of n‐Phenyl‐p‐Phenylenediammine as Novel Multifunctional Lubricant Additive for Deposit Control and Lubricity
Authors: Faujdar Ekta , Negi Himani , Bhonsle Aman , Atray Neeraj , Singh Raj K. 
Published: September 2020

AbstractA novel multifunctional amide additive N‐phenyl‐p‐phenylenediamine‐dodecenylsuccinic anhydride (PPA‐DDSA) was synthesized by the reaction of DDSA and N‐phenyl‐p‐phenylenediamine. The successful synthesis of amide additive was confirmed by various analytical techniques like Fourier Transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and thermogravimetric analysis. Performance of prepared additive was evaluated as multifunctional additive for anti‐oxidant, detergent dispersant, anti‐corrosion, and lubricity properties. The MAK‐500® mineral base oil and synthetic polyol base oil were used as a reference. The antioxidant property was evaluated by using the 2,2‐diphenyl‐1‐picrylhydrazyl method, which revealed that PPA‐DDSA was better antioxidant than PPA and gallic acid but less effective than butylated hydroxytoluene. PPA‐DDSA was found to be excellent detergent dispersant in polyol in comparison to the MAK‐500. At 4000 ppm additive concentration, the coking value is reduced to 0.0205 g from the value of 0.0840 g for blank polyol while the % dispersancy increased to 40.67 from 20.00 for polyol base oil. The anti‐wear property was observed to be better in the MAK‐500 while the antifriction potential was found to be better in polyol base oil. The average wear scar is reduced from 881 to 550 μM at 2000 ppm concentration in MAK‐500 base oil, while at 3000 ppm PPA‐DDSA concentration, the average friction coefficient reduces to 0.0826 from 0.1276 for blank polyol.
Aggregation and Adsorption Behavior of Cationic Surfactants (Cetyltrimethylammonium Bromide and Dodecyltrimethylammonium Bromide) in Aqueous Solutions of Antibiotic Drug (Amikacin Sulphate) at Different Temperatures
Authors: Chauhan Suvarcha , Chaudhary Poonam , Sharma Vivek 
Published: September 2020

AbstractConductivity and spectroscopy techniques have been accomplished to comprehend the mechanism of supramolecular assembly of cetyltrimethylammonium bromide (CTAB) and dodecyltrimethylammonium bromide (DTAB) in aqueous solution of amikacin sulphate (0.001, 0.005, and 0.010 mol kg−1). For CTAB, the normal boost of the CMC value with rise in temperature manifests the significant role of aquaphilic dehydration. However, the aquaphobic dehydrations become prominent with temperature and depict typical U‐shaped behavior of CMC for DTAB. The thermodynamic parameters of micellization have been derived from CMC values. The outcomes have been conferred in terms of solvation of hydrophobic part of surfactants by hydrophobic part of amikacin sulphate and micellization becomes more favorable for surfactant with more hydrophobic character in the presence of drug. The alteration in micro‐environment of the ternary (drug/surfactant/water) system has been explained in terms of fluorescence emission intensity of surfactant solutions which has been found to decrease by the addition of drug. The obtained absorbance spectrum by varying concentrations of surfactant/drug affords noteworthy information regarding the diverse interactions in studied systems. Moreover, the exhaustive understanding of surfactant micellar behavior have been discussed in consideration of use of surfactants as drug delivery agents and hence to amplify drug bioavailability consequently remodeling its treatment efficacy.
Structural characterization of calcium‐binding sunflower seed and peanut peptides and enhanced calcium transport by calcium complexes in Caco‐2 cells
Authors: Bao Xiaolan , Yuan Xingyu , Feng Guoxue , Zhang Meili , Ma Sarina 
Published: September 2020

AbstractBACKGROUNDPeptide–Ca complexes can promote Ca absorption. The present study aimed to determine the transport mechanism and structural characteristics of sunflower seed and peanut peptides with high Ca binding capacity with respect to developing third‐generation Ca supplements and functional food ingredients.RESULTSHigh Ca‐binding fractions of 1–3 kDa sunflower seed peptide (SSP4) and ≥ 10 kDa peanut peptide (PP1) had higher amount of Ca transported than CaCl2 and two hydrolyzed proteins in Caco‐2 cells. SSP4 and PP1 were separated by Ca ion metal chelate affinity chromatography, and high Ca‐binding fractions were observed for SSP4‐P2 and PP1‐P2. The amino acid sequences of SSP4‐P2 and PP1‐P2 were characterized by high‐performance liquid chromatography‐electrospray ionization‐time of flight mass spectrometry. Seven and eight peptides were identified from SSP4‐P2 and PP1‐P2, respectively. These peptides had molecular weights ranging from 1500 Da to 2500 Da and a large number of characteristic amino acid sequences, such as EEEQQQ, EQ–QQQ–QQ, QQ–QQQQQ, E–EEE, EE–EEQ, RR, Q–QQ–QQQ, EE–EQ–EE–Q, QQ–QQQQ, and Q–QQQQ, where ‘E’ is glutamic acid and ‘Q’ is glutamine.CONCLUSIONSSP4 and PP1 can promote Ca transport in Caco‐2 cells without affecting cell permeability. The amino acid sequences of SSP4‐P2 and PP1‐P2 with high Ca‐binding abilities contain characteristic sequences, such as continuous glutamic acid and glutamine, and have low molecular weights. © 2020 Society of Chemical Industry
Can drip irrigation under mulch be replaced with shallow‐buried drip irrigation in spring maize production systems in semiarid areas of northern China?
Authors: Geng Yuhui , Cao Guojun , Wang Lichun , Wang Meng , Huang Jinxin 
Published: September 2020

AbstractBACKGROUNDIn recent years, shallow‐buried drip irrigation in spring maize production has gradually replaced drip irrigation under plastic films and has become a common irrigation method in semiarid areas of northern China.RESULTTwo years of field experiments were carried out in the semiarid area of western Jilin province to compare the two drip irrigation methods in the spring maize production system. The treatments included MW1 (drip irrigation under mulch + moderate irrigation amount), MW2 (drip irrigation under mulch + high irrigation amount), SM1 (shallow‐buried drip irrigation + moderate irrigation amount), and SM2 (shallow‐buried drip irrigation + high irrigation amount). The maize yields were significantly higher under mulch than under shallow‐buried drip irrigation, but there was no interaction between mulch and the irrigation amount. Drip irrigation under mulch greatly improved spring maize N, P and K uptake compared with that under shallow‐buried drip irrigation. The agronomic‐use efficiency, recovery‐use efficiency, and partial factor productivity were ranked in the order of MW2 > MW1 > SW2 > SW1. The water‐use efficiency of drip irrigation under mulch was 7.44% and 6.82% higher than that of shallow‐buried drip irrigation under the moderate and high irrigation levels, respectively. However, considering the costs of the plastic mulch, there was no significant (P < 0.05) difference in economic benefits between the SW and MW treatments.CONCLUSIONDrip irrigation under plastic film provides greater advantages for production, but shallow‐buried drip irrigation may be a suitable method for farmers until fertigation technology is further optimized and the problem of plastic film pollution is solved. © 2020 Society of Chemical Industry
Combining ethanol pre‐treatment and ultrasound‐assisted drying to enhance apple chips by fortification with black carrot anthocyanin
Authors: Rojas Meliza Lindsay , Augusto Pedro Esteves Duarte , Cárcel Juan Andrés 
Published: September 2020

AbstractBACKGROUNDAn interesting approach to improve dried foods nutritional properties, functionality, and sensorial attributes, is by taking advantage of pre‐treatments for incorporating components into the food matrix. Based on this, this work studied the incorporation of black carrot anthocyanins in apple tissue by using ethanol (concentrations 0–300 mL L−1) as a pre‐treatment to ultrasound‐assisted convective drying. Samples were pre‐treated in acidified ethanol solutions, with and without anthocyanins, and then dried (50 °C, 1 m s−1) by convective and ultrasound‐assisted convective (21.77 kHz, 20.5 kW m−3) drying. Both the drying process improvement and the obtained product properties were studied.RESULTSThe anthocyanins did not influence the drying kinetics. In contrast, time reduction was > 50% by using both ethanol pre‐treatments and ultrasound. Ethanol pre‐treatments decreased the external resistance to mass transfer, while ultrasound decreased both internal and external resistances. The impregnation increased the anthocyanins (above 947%), which were retained after drying. Colour modifications after pre‐treatments and after drying (L*, b*, h° decrease, and a* increase), and antioxidant capacity retention were observed in samples with anthocyanin addition.CONCLUSIONThe results point that ethanol pre‐treatments and ultrasound application can accelerate drying, and through the natural colouring incorporation during pre‐treatments, the nutritional properties of dried samples were better retained. © 2020 Society of Chemical Industry
Patient generated health data and electronic health record integration in oncologic surgery: A call for artificial intelligence and machine learning
Authors: Melstrom Laleh G. , Rodin Andrei S. , Rossi Lorenzo A. , Fu Paul , Fong Yuman , Sun Virginia 
Published: September 2020

AbstractIn this review, we aim to assess the current state of science in relation to the integration of patient‐generated health data (PGHD) and patient‐reported outcomes (PROs) into routine clinical care with a focus on surgical oncology populations. We will also describe the critical role of artificial intelligence and machine‐learning methodology in the efficient translation of PGHD, PROs, and traditional outcome measures into meaningful patient care models.
Nodal characteristics associated with adverse prognosis in oral cavity cancer are linked to host immune status
Authors: Valero Cristina , Zanoni Daniella K. , Pillai Anjali , Xu Bin , Katabi Nora , Ghossein Ronald A. , Ganly Ian , Morris Luc G. T. , Shah Jatin P. , Wong Richard J. , Patel Snehal G. 
Published: September 2020

AbstractBackground and ObjectiveNodal metastasis is one of the strongest predictors of outcomes in oral cavity squamous cell carcinomas (OSCC). The aim was to analyze the interplay of nodal characteristics in OSCC prognosis.MethodsIn this retrospective cohort study we included OSCC patients treated with primary surgery including neck dissection between 2005 and 2015 (n = 619). Disease‐specific survival (DSS) was the primary endpoint. Optimal cutoffs were identified using recursive‐partitioning analysis (RPA). A novel characteristic—metastatic focus‐to‐lymph node size ratio (MLR)—was introduced. We compared the American Joint Committee on Cancer, Eighth Edition (AJCC8) pN categories to a new categorization.ResultsPatients with higher neutrophil‐to‐lymphocyte ratio had more adverse nodal characteristics. All nodal characteristics were significant predictors of DSS in univariable analysis. In multivariable analysis, only number of positive nodes and MLR remained significant. An RPA including all nodal covariates confirmed the results. Compared with AJCC8, our RPA categorization had better hazard discrimination (0.681 vs. 0.598), but poorer balance value (0.783 vs. 0.708).ConclusionPatients with higher neutrophil‐to‐lymphocyte ratio had more adverse nodal characteristics. Total number of metastatic lymph nodes is the strongest predictor of outcomes in OSCC. MLR is a more powerful predictor than metastatic lymph node size or metastatic focus size alone.
Impact of ablation algorithm versus tumor‐dependent parameters on local control after microwave ablation of malignant liver tumors
Authors: Erten Ozgun , Li Pengpeng , Gokceimam Mehmet , Akbulut Serkan , Berber Eren 
Published: September 2020

AbstractBackgroundThe aim of this study is to assess the effect of tumor versus ablation‐algorithm dependent parameters on local recurrence (LR) after microwave ablation (MWA) of liver malignancies.MethodsThis was an institutional review board‐approved study of patients who underwent laparoscopic or open MWA of malignant liver tumors. The impact of ablation algorithm (stepwise or direct heating, single or overlapping ablations, and ablation margin) and tumor‐dependent (type, size, location, and blood vessel proximity) parameters on LR was analyzed using Kaplan–Meier and Cox proportional hazards.ResultsA total of 179 patients with 602 liver tumors underwent 200 MWA procedures. Colorectal liver metastasis (CLM) was the most frequent tumor type followed by neuroendocrine liver metastasis (NELM), other metastatic tumors, and hepatocellular cancer (HCC). For patients followed at least a year with imaging, LR rate was 8.8% per lesion and 13.1%,1.3%, 11.7%, and 12.6%, for CLM, NELM, HCC, and other tumor types, respectively. On multivariate analysis, independent predictors of LR included tumor type, tumor size, and ablation margin.ConclusionLR after MWA for malignant liver tumors is predicted by both tumor and surgeon‐dependent factors. Variations in the ablation algorithm did not affect LR, leaving the ablation margin as the only parameter that could be modified to optimize local tumor control.
Simultaneous detection and quantification of 57 compounds in Spatholobi Caulis applying ultra‐fast liquid chromatography with tandem mass spectrometry
Authors: Liu Xiao‐Yan , Zhang Lei , Yang Xiu‐Wei , Zhang You‐Bo , Xu Wei , Zhang Peng , Zhao Wei , Peng Kai‐Feng , Gong Yun , Liu Ni‐Fu 
Published: September 2020

AbstractA method of ultra‐fast liquid chromatography in series with tandem mass spectrometry for the rapid and sensitive detection of 57 compounds in Spatholobi Caulis (the vine stem of Spatholobus suberectus Dunn) within 35 min was established. This assay can simultaneously determine a variety of compounds without matrix interference in multiple reaction monitoring mode including evaluating the quality of different batches of Spatholobi Caulis from several areas and further identifying the characteristic compounds efficiently. After comprehensive validation, this method can be used to determinate samples rapidly, precisely, accurately, repeatably, and sensitivity. There were significant content differences in 12 batches of Spatholobi Caulis, which were further classified and systematically differentiated applying multivariate statistical analysis. Furthermore, orthogonal partial least squares discrimination analysis results indicated that (−)‐gallocatechin (10), (−)‐epiafzelechin (20), 4,7,2′‐trihydroxy‐4′‐methoxyisoflavanol (51), and biochanin A (53) characterize compounds to discernment internal quality of Spatholobi Caulis, and recommended as quality control indicators. Hence, presented work provides a method for further study on pharmaceutic preparation, metabolism, as well as for the design, production optimization process, and clinical application.
Recent developments in the characterization of nucleic acids by liquid chromatography, capillary electrophoresis, ion mobility, and mass spectrometry (2010–2020)
Authors: Santos Inês C. , Brodbelt Jennifer S. 
Published: September 2020

AbstractThe development of new strategies for the analysis of nucleic acids has gained momentum due to the increased interest in using these biomolecules as drugs or drug targets. The application of new mass spectrometry ion activation techniques and the optimization of separation methods including liquid chromatography, capillary electrophoresis, and ion mobility have allowed more detailed characterization of nucleic acids and oligonucleotide therapeutics including confirmation of sequence, localization of modifications and interaction sites, and structural analysis as well as identification of failed sequences and degradation products. This review will cover tandem mass spectrometry methods as well as the recent developments in liquid chromatography, capillary electrophoresis, and ion mobility coupled to mass spectrometry for the analysis of nucleic acids and oligonucleotides.
A randomized pilot study on self‐management in head and neck lymphedema
Authors: McLaughlin Tess M. , Broadhurst Jane J. , Harris Courtenay J. , McGarry Sarah , Keesing Sharon L. 
Published: September 2020

AbstractBackgroundThe purpose of this pilot trial was to determine the feasibility of a self‐managed lymphedema randomized control trial to test the effectiveness of a head and neck‐specific exercise protocol.MethodsNine participants were randomized to receive usual treatment provided by an Australian metropolitan teaching hospital (n = 4) or usual treatment with an added head and neck exercise regime (n = 5). Feasibility was assessed through ease of recruitment, adherence, and safety. Lymphedema reduction and quality of life (QOL) data were assessed at baseline (0 week) and follow‐up (6 weeks).ResultsThe study was feasible in terms of safety and participant retention. However, a slow recruitment rate and low adherence may impact future trials. There were no significant differences in lymphedema reduction or QOL between groups.ConclusionThis pilot feasibility study demonstrated that a self‐management trial can be implemented, however, modifications will be required due to the slow recruitment and poor adherence rates.Level of evidence1b: Individualized randomized control trial.
Habitat‐specific biogenic production and erosion influences net framework and sediment coral reef carbonate budgets
Authors: Brown Kristen T. , Bender‐Champ Dorothea , Achlatis Michelle , van der Zande Rene M. , Kubicek Andreas , Martin Storm B. , Castro‐Sanguino Carolina , Dove Sophie G. , Hoegh‐Guldberg Ove 
Published: September 2020

AbstractCarbonate budgets are increasingly being used as a key metric to establish reef condition. To better understand spatial variations in framework and sediment net carbonate budgets, we quantified biogenic carbonate production, erosion, and dissolution within and between five distinct geomorphological habitats of Heron Reef on the southern Great Barrier Reef. The protected reef slope had the greatest estimated net framework carbonate budget (22.6 kgCaCO3 m−2 yr−1 ± 2.4 SE), driven by abundant, fast‐growing acroporid corals coupled with low levels of macro‐ and micro‐bioerosion. The estimate of the exposed reef slope was significantly lower due to localized damage from a single tropical cyclone that occurred 7 years prior to this study (9.7 kgCaCO3 m−2 yr−1 ± 2.8 SE). Within the extensive lagoon, net framework carbonate budgets ranged from 0.24 kgCaCO3 m−2 yr−1 (± 0.1 SE) to 3.0 kgCaCO3 m−2 yr−1 (± 0.7 SE). The greatest net sediment carbonate budget was estimated within the reef crest (6.0 kgCaCO3 m−2 yr−1 ± 1.1 SE) and the lowest in the shallow lagoon (1.2 kgCaCO3 m−2 yr−1 ± 0.2 SE). Chemical dissolution of the sediments exhibited spatial variability, with reef crest and reef flat sediments in a state of net production. Considering the area of each habitat, the net reef framework and sediment budgets across Heron Reef were 4.06 kgCaCO3 m−2 yr−1 and 2.82 kgCaCO3 m−2 yr−1, respectively. The results of this study improve our understanding of spatial variability in carbonate production and bioerosion and provide a comprehensive reef‐scale carbonate budget for a relatively undisturbed coral reef ecosystem.
Dextroplantation of Left Liver Graft in Infants
Authors: Ahn Sung Woo , Yi Nam‐Joon , Kim Hyo‐Cheol , Ahn Hye Young , Hong Suk Kyun , Lee Jeong‐Moo , Cho Jae‐Hyung , Jin Ung Sik , Chang Hak , Lee Myungsu , Hur Saebeom , Jae Hwan Jun , Jung Chul‐Woo , Yang Seong‐Mi , Ko Jae Sung , Lee Kwang‐Woong , Suh Kyung‐Suk 
Published: September 2020

The position of the left side liver graft is important, and it could lead to complications of the hepatic vein (HV) and portal vein (PV), especially in a small child using a variant left lateral section (vLLS) graft. The purpose of this study was to evaluate the outcome of a novel technique for the implantation of a vLLS graft to the right side (dextroplantation) in infants. For 3 years, 10 consecutive infants underwent dextroplantation using a vLLS graft (group D). The graft was implanted to the right side of the recipient after 90° counterclockwise rotation; the left HV graft was anastomosed to inferior vena cava using the extended right and middle HV stump, and PV was reconstructed using oblique anastomosis without angulation. Surgical outcomes were compared with the historical control group (n = 17, group C) who underwent conventional liver transplantation using a vLLS during infancy. Group D recipients were smaller than group C (body weight <6 kg: 50.0% versus 11.8%; P = 0.03). The rate of graft‐to‐recipient weight ratio >4% was higher in group D (60.0%) than C (11.8%; P = 0.01). Surgical drains were removed earlier in group D than in group C (15 versus 18 postoperative days [PODs]; P = 0.048). Each group had 1 PV complication (10.0% versus 5.9%); no HV complication occurred in group D, but 3 HV complications (17.6%) occurred in group C (P > 0.05). Hospital stay was shorter in group D than in group C (20 versus 31 PODs; P = 0.02). Dextroplantation of a vLLS graft, even a large‐for‐size one, was successful in small infants without compromising venous outcomes, compared with conventional vLLS transplantation. We could remove the surgical drains earlier and reduce hospital stays in cases of dextroplantation.