bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation on the Interaction between Tetrakis (4-carboxylphenyl) Porphyrin and CopC by Spectroscopy and Docking Methods
Authors: Song Zhen , Liu Jin , Yuan Wen , Bai Ze , Gao Nvwa 
Published: December 2019

Background:
Recently, the small molecule that inhibits the human copper-trafficking
proteins Atox1 and CCS was reported, which suggested that small molecule has an effect on the
copper regulation system in the cell. The copper chaperones CopC is regarded as a redox switch
and possess barrel structure, thus the interaction between CopC and small molecules could give
helpful information to elucidate the copper regulation mechanism. In addition, porphyrins play an
important role in the metabolism of living body. In the early-stage tumors, porphyrins were usually
used to diagnosis. After the amphiphilic porphyrins were given by intravenous injection, serum
albumins and serum proteins were the most usual carrier to transfer them. Then these molecules can
accumulate in malignant tumours and contact with cancer cells. Obviously, in drug distribution and
efficacy, investigation of the interaction between the porphyrins and protein is an important
research area. Obviously, in drug distribution and efficacy, investigation of the interaction between
the porphyrins and protein is an important research area.

Objective:
In this article, our motivation is to establish a relation between Tetrakis (4-
carboxylphenyl) porphyrin and CopC.

Methods:
In this article, we propose a framework for achieving our aforementioned object. Firstly,
FTIR spectra and CD were used to detect the structure changes of CopC. Secondly, the
fluorescence spectroscopic and UV-Vis spectra were used to measure quenching mechanism,
binding distance, binding site and binding distance. Using Tb 3+ as a probe to detect the interaction
between CopC and TCPP. Finally, molecular docking methods was used to show the results more
vivid.

Result:
Following the proposed framework, firstly, FTIR and CD results indicated that the CopC
conformation was changed by TCPP. The β-sheet content was reduced and the random coil content
was increased. Secondly, fluorescence spectra data indicated that the combination ratio of TCPPCopC
was 1:1, and the inclusion constant is (5.88 ± 0.12) × 10 5 M -1 . In addition, Tb 3+ was used
as a probe to detect the interaction between CopC and TCPP. The result further verified that CopC
can interact with TCPP. The thermodynamic parameters of interaction between CopC and TCPP
(ΔH, ΔS) indicated that the force between CopC and TCPP was mainly hydrophobic interaction.
Finally, the distance between tryptophan in CopC and TCPP was calculated through forster energy
transfer and molecular docking.

Conclusion:
The results revealed that TCPP can form 1:1 complex with CopC, and the binding
constant has been calculated to be (5.88 ± 0.12) × 10 5 M -1 . In addition, it was revealed that
TCPP quench the fluorescence of CopC by the static quenching mechanism and the binding site n
equals one. The formation of CopC-TCPP complex depended on the hydrophobic force and the
distance between TCPP and tryptophan residue in CopC was 2.07 nm.
Dual Inhibition of DPP-4 and Cholinesterase Enzymes by the Phytoconstituents of the Ethanolic Extract of Prosopis cineraria Pods: Therapeutic Implications for the Treatment of Diabetes-associated Neurological Impairments
Authors: Ram Heera , Jaipal Noopur , Kumar Pramod , Deka Purbajyoti , Kumar Shivani , Kashyap Priya , Kumar Suresh , Singh Bhim P. , Alqarawi Abdulaziz A. , Hashem Abeer , Tabassum Baby , Abd-Allah Elsayed Fathi 
Published: December 2019

Background:Insulin resistance causes decreased uptake of glucose which promotes the susceptibility of type 2 associated neurological impairments.Methods:The study was aimed to evaluate the inhibition potential of the ethanolic extract of Prosopis cineraria (EPC) pods against DPP-4 and cholinesterase enzymes by in-vitro, in-vivo and in-silico assessments. The present study consists of in vivo studies on a diabetes-induced rat model by HOMA (Homeostasis model assessment) and related parameters, in vitro studies through the DPP-4 enzyme assay and cholinesterase assays using Ellman’s reaction. The in-silico studies were conducted by the molecular docking of Cinerin C with targeted enzymes. The phytochemical characterization of the extract was demonstrated through LCMS studies. The antioxidant studies on the extract were performed by FRAP and TEAC assays.Results:The extract showed 64.8% maximum inhibition of DPP-4, 34.91% inhibition of AChE and 74.35% inhibition of BuChE. The antioxidant capacity of the extract was observed to be 847.81±16.25μM Fe2+ equivalent in the FRAP assay and 0.40 ± 0.08 mmol/l of Trolox equivalent in the TEAC assay. The in vivo study showed competent glycaemic control against significant HOMA IR (1.5), HOMA % β (26.5) and HOMA % S (68.8) as well as pancreatic cell mass proliferation. The insilico analysis also revealed positive interactions of Cinerin C with targeted enzymes (DPP4 and cholinesterase).Conclusion:It can be concluded that the phytoconstituents of Prosopis cineraria pod extract can be significantly considered in neuropharmacology to resolve insulin resistance-induced neurological complications as it showed inhibition against DPP-4, AChE and BuChE target enzymes.
Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate
Authors: Samini Laleh , Khoubnasabjafari Maryam , Alimorad Mohamad M. , Jouyban-Gharamaleki Vahid , Chan Hak-Kim , Jouyban Abolghasem 
Published: December 2019

Background:
Analysis of drug concentrations in biological fluids is required in clinical
sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is
a potential sample for follow up of drug concentrations.

Methods:
A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated
liquid chromatography method was employed for the determination of budesonide (BDS) in EBC
samples collected using a homemade setup. EBC is a non-invasive biological sample with possible
applications for monitoring drug concentrations. The proposed analytical method is validated according
to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected
from healthy volunteers receiving a single puff of BDS.

Results:
The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent,
chloroform (200 μL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The
method was validated over a concentration range of 21-210 μg·L-1 in EBC. Inter- and intra-day precisions
were less than 10% where the acceptable levels are less than 20%. The validated method was
successfully applied for the determination of BDS in EBC samples.

Conclusion:
The findings of this study indicate that the developed method can be used for the extraction
and quantification of BDS in EBC samples using a low cost method.
CNTs Supercapacitor Based on the PVDF/PVA Gel Electrolytes
Authors: Pour Gobad B. , Aval Leila F. , Mirzaee Masoud 
Published: December 2019

Background:
In this paper, the supercapacitor based on the carbon nanotubes (CNTs) electrodes
has been fabricated.

Objective:
The Polyvinylidene Fluoride (PVDF) and Polyvinyl Alcohol (PVA) were used as a gel electrolyte.

Methods:
The electrodes and electrolytes thin films were characterized by Scanning Electron Microscopy
(SEM) and Transmission Electron Microscopy (TEM). The specific Capacitance (Cs) of the
CNTs-based supercapacitor has been measured using the cyclic voltammetry and galvanostatic methods.
For the scan rate, 20 mV s-1 the Cs of the CNTs-based supercapacitor was 173 F g-1.

Results:
Using the electrochemical impedance spectroscopy the Nyquist curve has been plotted. The
reactance capacitance and the equivalent series resistance of the CNTs-based supercapacitor with
PVDF/PVA gel electrolytes were 90 Ω and 25 Ω respectively.

Conclusion:
Also, few patents for the CNTs-based supercapacitor have been reviewed and cited. The
CNTs-based supercapacitor proposed a new structure solid-state and flexible supercapacitor with high
performance.
Smooth Coated Otter Optimizer: A New Nature Inspired Optimizer
Authors: Rajani  , Kumar Naresh , Kaswan Kuldeep Singh 
Published: December 2019

Background:
Nature inspired Algorithms have become predominant from the last few decades due to their derivative free nature and capability of avoiding the local optima problem. These algorithms have already shown humongous success in software reliability estimation. Objective: A new nature inspired algorithm inspired by the hunting capability of smooth coated otter’s for parameter optimization of software reliability models is proposed. Hunting in group is the most striking feature of the otters. In this paper, smart hunting behavior of otter’s is implemented to optimize software reliability model parameters.

Method:
Otters strongly co-operate together and show ideal team cooperation. Intelligent fish hunting capability of smooth coated otters makes them different from other swarm intelligence based algorithms. Three phases of otter hunting mainly moving in V formation in the direction of movement of the prey, approaching violently through the creek and then attacking the prey on the shore are implemented. Three software reliability models are used for showing the suitability of proposed algorithm.

Result:
The evaluation of study shows that proposed algorithm beat ABC, GA and PSO algorithms by 75% and 50% in terms of lower value of SSE and lower value of MSE.

Conclusion:
Intelligent foraging behavior of smooth coated otter provides significant gain capability in parameter estimation of software reliability models. The results are motivating. Further smooth coated otter optimizer can be applied to other optimization problems.
Hubungan somatotype dengan kesegaran jasmani atlet sepak bola
Authors: Anggitasari Elok Dwi , Dieny Fillah Fithra , Candra Aryu 
Published: December 2019

Penelitian ini bertujuan untuk mengetahui hubungan somatotype dengan kesegaran jasmnai atlet sepak bola. Desain penelitian cross sectional dengan sampel sebanyak 42 subjek dipilih secara random sampling. Data somatotype didapatkan dari pengukuran antropometri terdiri dari berat badan, tinggi badan, trisep, suprailiaca, subscapular, calf skinfold, humerus width, fumerus width, dan flixed arm girth yang dihitung dengan metode antropometri Heath-Carter untuk menghasilkan skor somatotype yaitu endomorph, mesomorph, dan ectomorph. Data kesegaran jasmani diperoleh dengan tes ACSPFT (Asian Committee on the Standarization of Physical Fitness Test) terdiri dari tes kecepatan, daya ledak otot, ketangkasan, kelenturan, dan kekuatan. Data asupan zat gizi diperoleh dengan metode food recall 3x24 jam. Data dianalisis menggunakan uji chi-square dan kolmogorov-smirnov. Dari semua subjek diperoleh 19% memiliki tipe tubuh endomorph, 26.2% mesomorph, dan 54.8% ectomorph. Secara keseluruhan subjek memiliki kesegaran jasmani 73.8% baik, 21.4% sedang, dan 4.8 % kurang. Ada hubungan somatotype dengan kecepatan (p=0,034), daya ledak otot (p=0.0001), ketangkasan (p=0.0001), kelenturan (p=0.041), kekuatan (p=0.003) dan kesegaran jasmani (p=0.045). Tipe ectomorph dan dan mesomorph memiliki komponen kesegaran jasmani lebih baik dibandingkan tipe endomorph. Asupan energi (p=0.035) dan somatotype (p=0.045) merupakan variabel yang paling berpengaruh terhadap kesegaran jasmani Correlation of Somatotype with Physical Fitness of Football AthletesAbstractThe aim of this study is to analyze the correlation of somatotype with physical fitness in football athletes. A cross sectional study with 42 subjects was selected by random sampling. Somatotype assessed with anthropometric measurements consists of weight, height, tricep, suprailiaca, subscapular, skinfold calf, humerus width, fumerus width, flixed arm girth. The somatotype components were calculating Heath-Carter anthropometric method to obtained somatotype score endomorph, mesomorph, and ectomorph. Physical fitness were obtained by the ACSPFT (Asian Committee on the Standarization of Physical Fitness Test) consists of speed, muscular explosive power, agility, flexibility, and strength. Data of nutrient intake were obtained by food recall method 3x24 hours. Data analyzed by chi-square and kolmogorov-smirnov test. Subjects were 19% endomorph, 26.2% mesomorph, and 54.8% ectomorph. Overall subjects had a good physical fitness 73.8%, 21.4% moderate, and 4.8% less. There were various somatotype correlation with speed (p=0.034), muscle explosive power (p=0.0001), agility (p=0.0001), flexibility (p=0.041), strength (p=0.003) and physical fitness (p=0.045). The ectomorph and mesomorph types had better physical fitness components than the endomorph type. Energy intake (p=0.035) and somatotype (p=0.045) were the most influential variables on physical fitness.
Factors Associated With Trial Completion and Adherence in App-Based N-of-1 Trials: Protocol for a Randomized Trial Evaluating Study Duration, Notification Level, and Meaningful Engagement in the Brain Boost Study
Authors: Bobe Jason R , Buros Jacqueline , Golden Eddye , Johnson Matthew , Jones Michael , Percha Bethany , Viglizzo Ryan , Zimmerman Noah 
Published: December 2019

Background
N-of-1 trials promise to help individuals make more informed decisions about treatment selection through structured experiments that compare treatment effectiveness by alternating treatments and measuring their impacts in a single individual. We created a digital platform that automates the design, administration, and analysis of N-of-1 trials. Our first N-of-1 trial, the app-based Brain Boost Study, invited individuals to compare the impacts of two commonly consumed substances (caffeine and L-theanine) on their cognitive performance.

Objective
The purpose of this study is to evaluate critical factors that may impact the completion of N-of-1 trials to inform the design of future app-based N-of-1 trials. We will measure study completion rates for participants that begin the Brain Boost Study and assess their associations with study duration (5, 15, or 27 days) and notification level (light or moderate).

Methods
Participants will be randomized into three study durations and two notification levels. To sufficiently power the study, a minimum of 640 individuals must begin the study, and 97 individuals must complete the study. We will use a multiple logistic regression model to discern whether the study length and notification level are associated with the rate of study completion. For each group, we will also compare participant adherence and the proportion of trials that yield statistically meaningful results.

Results
We completed the beta testing of the N1 app on a convenience sample of users. The Brain Boost Study on the N1 app opened enrollment to the public in October 2019. More than 30 participants enrolled in the first month.

Conclusions
To our knowledge, this will be the first study to rigorously evaluate critical factors associated with study completion in the context of app-based N-of-1 trials.

Trial Registration
ClinicalTrials.gov NCT04056650; https://clinicaltrials.gov/ct2/show/NCT04056650

International Registered Report Identifier (IRRID)
PRR1-10.2196/16362
Влияние артериальной гипертензии на течение аортального стеноза у пациентов, перенесших протезирование аортального клапана
Authors: Zhadan A.V. 
Published: December 2019

Цель работы: изучить влияние артериальной гипертензии (АГ) на течение стеноза аортального клапана (АК), оценить выраженность процессов ремоделирования сердца в зависимости от наличия АГ у пациентов, перенесших протезирование АК. Материалы и методы. В исследование были включены 72 пациента, перенесшие протезирование аортального клапана по поводу аортального стеноза. Средний возраст включенных в исследование больных составил 63,9 ± 11,8 года; мужчин было 50, женщин — 22. У 6 пациентов отмечался двухстворчатый АК. В зависимости от наличия или отсутствия АГ пациенты были разделены на 2 группы. Первую группу составили пациенты, у которых была диагностирована АГ (37 человек). Средний возраст пациентов данной группы — 67,9 ± 8,5 года; мужчин было 27, женщин — 10. Вторая группа пациентов не имела АГ в анамнезе. Количество пациентов данной группы — 35 человек, средний возраст которых составил 59,3 ± 13,5 года; мужчин было 22, женщин — 13. Результаты. В группе пациентов без АГ через 12 мес. после кардиохирургического вмешательства отмечалось статистически значимое снижение конечно-систолического размера левого желудочка по сравнению с исходным дооперационным показателем: 41,6 ± 4,6 мм — до операции и 34,0 ± 6,0 мм — через 1 год. Также в этой группе пациентов наблюдалось статистически значимое увеличение фракции выброса: 47,0 ± 8,2 % — до вмешательства и 59,8 ± 7,8 % — через 12 мес. после вмешательства. Подобных изменений не отмечалось в группе пациентов с АГ. Также в группе пациентов с АГ после протезирования клапана не отмечалось уменьшение массы миокарда левого желудочка, тогда как во второй группе произошло статистически значимое снижение массы миокарда левого желудочка с 237,0 ± 57,5 г до 192,2 ± 53,4 г через 12 мес. после операции. Выводы. Наличие АГ при аортальном стенозе не влияет на предоперационное течение аортального стеноза и не является дополнительным фактором, способствующим развитию более выраженной гипертрофии левого желудочка. Наличие АГ влияет на постоперационное ремоделирование сердца.
Нейропротекція після системного тромболізису в пацієнтів з ішемічним інсультом: огляд клінічних випадків
Authors: Pasichnyk H.P. , Martynchuk Yu.N. , Orel V.M. 
Published: December 2019

У даній роботі поданий огляд клінічних випадків ішемічного інсульту, лікування яких проводилося з використанням системного тромболізису й високих доз Церебролізину з метою нейропротекції. Особливу увагу автор приділяє ефективності такої комбінації. Поєднання тромболітичної терапії та нейропротекції — безпечний і ефективний метод лікування інсульту. Поєднання тромболітичної терапії з Церебролізином у максимальних лікувальних дозах демонструє високу потужність і швидкість терапевтичного ефекту, відсутність побічної дії та ускладнень, дозволяє зменшити розмір інфаркту, запобігає утворенню й поширенню набряку мозку, стабілізує мікроциркуляцію, нормалізує неврологічні й когнітивні порушення.
Синдром избыточного бактериального роста у больных с хроническими диффузными заболеваниями печени в зависимости от этиологии и морфологических особенностей
Authors: Didenko V.I. , Zigalo, E.V. , Gaidar Yu.A. , Yagmur V.B. 
Published: December 2019

Актуальность. Ряд исследований указывают на повышение риска появления нарушений со стороны печени при дисбиотических нарушениях в кишечнике. В свою очередь, изменение физиологических процессов в печени может стать толчком для возникновения синдрома избыточного бактериального роста (СИБР). Доказано, что СИБР в тонком кишечнике может играть определенную роль в патогенезе алкогольной болезни печени и осложнений, связанных с хроническим гепатитом С. Целью исследования было оценить частоту возникновения синдрома избыточного бактериального роста у больных хроническими диффузными заболеваниями печени (ХДЗП) с учетом степени стеатоза, стадии фиброза и степени активности воспалительного процесса в печени. Материалы и методы. 27 обследованных пациентов были распределены на 2 группы: I — 12 больных с хроническим гепатитом, ассоциированным с вирусом С (ХГС); II — 15 больных с алкогольной болезнью печени (АБП) в зависимости от этиологии при формировании и прогрессировании стеатоза и фиброза печени с использованием данных гистологических методов исследования, компьютерной морфометрии, транзиентной эластометрии и показателя степени стеатоза САР на аппарате FibroScan 502. Синдром избыточного бактериального роста определяли с помощью водородного дыхательного теста (ВДТ) с глюкозой. Результаты. СИБР был выявлен у 51,8 % больных с ХДЗП, достоверно почти в 1,5 раза преобладал у больных с АБП (60,0 %) по сравнению с ХГС, у которых только в 41,7 % случаев выявлен СИБР. Выявлено превалирование положительных случаев ВДТ в группе S3 (70,0 %), что достоверно отличалось от группы S1 (37,5 %). У больных ХГС с прогрессированием стеатоза увеличивается количество случаев СИБР, и наоборот, у большинства больных с АБП, у которых был выявлен гистологически подтвержденный микровезикулярный и мелкокапельный стеатоз печени, выявляли при ВДТ нарушения микробиоты в тонком кишечнике. Выводы. Между показателями водородного дыхательного теста и индексом гистологической активности, гистологическими показателями прогрессирования стеатоза печени у больных хроническим гепатитом С выявлена четкая взаимо­связь: с увеличением активности воспалительного процесса (9 баллов) и степени стеатоза печени (S3) повышается уровень нарушения микробиоты в тонком кишечнике (> 20 ppm), то есть при ХГС уровень выделения водорода адекватно отображает степень активности воспалительного процесса в печени.
Glucose and Counterregulatory Responses to Exercise in Adults With Type 1 Diabetes and Impaired Awareness of Hypoglycemia Using Closed-Loop Insulin Delivery: A Randomized Crossover Study
Authors: Lee Melissa H. , Vogrin Sara , Paldus Barbora , Jayawardene Dilshani , Jones Hannah M. , McAuley Sybil A. , Obeyesekere Varuni , Gooley Judith , La Gerche André , MacIsaac Richard J. , Sundararajan Vijaya , Jenkins Alicia J. , Ward Glenn M. , O’Neal David N. 
Published: December 2019

OBJECTIVE
To evaluate exercise-related glucose and counterregulatory responses (CRR) in adults with type 1 diabetes with impaired awareness of hypoglycemia (IAH) using hybrid closed-loop (HCL) insulin delivery to maintain glucose homeostasis.

RESEARCH DESIGN AND METHODS
Twelve participants undertook 45-min high-intensity intermittent exercise (HIIE) and moderate-intensity exercise (MIE) in random order. The primary outcome was continuous glucose monitoring (CGM) time in range (70–180 mg/dL) for 24-h post–exercise commencement.

RESULTS
CGM time in range was similar for HIIE and MIE (median 79.5% [interquartile range 73.2, 87.6] vs. 76.1% [70.3, 83.9], P = 0.37), and time with levels &lt;54mg/dL post–exercise commencement was 0%. HIIE induced greater increases in cortisol (P = 0.002), noradrenaline (P = 0.005), and lactate (P = 0.002), with no differences in adrenaline, dopamine, growth hormone, or glucagon responses.

CONCLUSIONS
IAH adults using HCL undertaking HIIE and MIE exhibit heterogeneity in CRR. Novel findings were a preserved cortisol response and variable catecholamine responses to HIIE.