bluuebunny/crossref_metadata_2025_split huggingface dataset:
More Twins in the Scientific Literature of the 21st Century
Authors: Mainkar Prathama S. , Vankamamidi Ambica , Chandrasekhar Srivari 
Published: March 2020

AbstractThis Essay highlights the complex issue of twinning in science publications. Historical accounts present cases where two scientists focused on the same problem and came up with the same solution following different paths. This has changed in the present day. The concurrent publication of rather similar research papers from different groups has increased in frequency since 2010. In the past, twinning in research publications was serendipitous, and there was a healthy competition among teams working on similar projects. Today, twinning has become more frequent. This can be attributed to the urge of researchers to have publications on popular topics, the tendency to base research programs on popular keywords, and funding agencies preferentially supporting certain areas of research. With vast amounts of literature being generated, editorial offices and referees may not be able to find these twins very easily. As we inch away from human ingenuity towards artificial intelligence, twinning may become even more frequent.
Multifunctional mussel‐inspired Gelatin and Tannic acid‐based hydrogel with pH‐controllable release of vitamin B12
Authors: Nath Jayashree , Saikia Priyanka Pratim , Handique Junali , Gupta Kuldeep , Dolui Swapan Kumar 
Published: March 2020

AbstractHydrogels have emerged to be an impeccable material for a large variety of applications over the past few decades. In the field of biomedical applications, remarkable progress has been observed in the effort of fabricating numerous hydrogel systems. In this work, gelatin and tannic acid‐based stretchable and adhesive hydrogel has been synthesized to study the release behavior of vitamin B12. Successful formation of the synthesized hydrogels was confirmed by Fourier transform infrared and X‐ray diffraction analysis. The morphology of the surfaces and the cross section of such hydrogels were studied with Scanning electron microscopy analysis. Swelling behavior of our hydrogel was studied with Design Expert software. The maximum swelling of the hydrogel was found to be around 137 g/g. Adhesive property was demonstrated on various surfaces to observe the adhesiveness of the fabricated hydrogel. Blood compatibility study was also performed. The release behavior of vitamin B12was performed in two different pH media and it was found to have enhanced value in the fluid mimicking the intestine. This work provides a new prospect for designing hydrogels with stretchable and adhesive properties with pH‐controllable drug delivery applications along with other promising applications in various fields of research.
Physical–chemical and structural stability of PHBV/wheat straw fibers based biocomposites under food contact conditions
Authors: Angellier‐Coussy Hélène , Kemmer Diana , Gontard Nathalie , Peyron Stéphane 
Published: March 2020

AbstractThis aricle aims at investigating for the first time the ability of using poly(3‐hydroxylbutyrate‐co‐3‐hydroxyvalerate) (PHBV)/wheat straw fibers (WSF) biocomposites as food contact materials for packaging applications. For that purpose, the impact of the filler size and content on overall migration and mechanical properties was assessed under standardized testing conditions using the food simulant liquids (FSL). Very high overall migration values were noticed in the case of hydrophilic FSL, that is, water, acetic acid 3% (wt/vol), ethanol 20% (vol/vol), and ethanol 95% (vol/vol), related to the leaching of water extractable components of WSF. Low migration values were obtained in contact with isooctane and olive oil, demonstrating the possibility of using biocomposites in contact with fatty products. To answer the lack of methodologies to simulate different types of food for which PHBV‐based composites could be more specifically dedicated, including food products of low or intermediate water activity such as fruits and vegetable, bread, and cheese, a new range of new solid food models based on agar‐agar gels have been developed. Results showed that the water activity (aw) was the main parameter governing the overall migration of PHBV/WSF materials, suggesting that such composite materials could only be used as food contact materials in the case of food products with an awequal or lower than 0.90.
Fishers, dams, and the potential survival of the world's rarest turtle, Rafetus swinhoei, in two river basins in northern Vietnam
Authors: Le Duc Olivier , Van Thong Pham , Leprince Benjamin , Bordes Cedric , Tuan Anh Nguyen , Benansio John Sebit , Pacini Nic , Luu Vinh Quang , Luiselli Luca 
Published: March 2020

Abstract

Next to cetaceans and megafishes, freshwater turtles are the most iconic endangered freshwater species.
A detailed questionnaire survey conducted with more than 100 individuals from fishing communities in northern Vietnam was used to investigate the current status of Southeast Asian turtles and provides new hope concerning the survival of Rafetus swinhoei, for which recent official records in the wild are limited to a single individual in Vietnam.
The survey included the entire Vietnamese portion of the Da River in Hoa Binh and Son La provinces, as well as the Chu and Ma river system in Thanh Hoa Province, as they are the last sites where the world's rarest and largest Asian softshell turtle has been seen. The questionnaire, conducted in Vietnamese, focused on demographic details, fishing intensity and gear, the status of fishing grounds, and the frequency of interaction with turtles.
The great majority of fishers could recognize different turtle species from photographs and describe their preferential breeding habitats; not all knew that they are protected. A few confirmed that more than once each year they still encounter freshwater turtles during their fishing activities.
This survey provides detailed information on sites where freshwater turtles are still seen in northern Vietnam and broadens our hope that wild individuals of the extremely rare R. swinhoei may still be present in the remaining riparian wetlands of these biodiverse, dammed, and controlled river basins in North Vietnam.
The personal information sphere: An integral approach to privacy and related information and communication rights
Authors: Eskens Sarah 
Published: March 2020

AbstractData protection laws, including the European Union General Data Protection Regulation, regulate aspects of online personalization. However, the data protection lens is too narrow to analyze personalization. To define conditions for personalization, we should understand data protection in its larger fundamental rights context, starting with the closely connected right to privacy. If the right to privacy is considered along with other European fundamental rights that protect information and communication flows, namely, communications confidentiality; the right to receive information; and freedom of expression, opinion, and thought, these rights are observed to enable what I call a “personal information sphere” for each person. This notion highlights how privacy interferences affect other fundamental rights. The personal information sphere is grounded in European case law and is thus not just an academic affair. The essence of the personal information sphere is control, yet with a different meaning than mere control as guaranteed by data protection law. The personal information sphere is about people controlling how they situate themselves in information and communication networks. It follows that, to respect privacy and related rights, online personalization providers should actively involve users in the personalization process and enable them to use personalization for personal goals.
Multi‐informant International Perspectives on the Facilitators and Barriers to Employment for Autistic Adults
Authors: Black Melissa H. , Mahdi Soheil , Milbourn Benjamin , Scott Melissa , Gerber Alan , Esposito Christopher , Falkmer Marita , Lerner Matthew D. , Halladay Alycia , Ström Eva , D'Angelo Axel , Falkmer Torbjorn , Bölte Sven , Girdler Sonya 
Published: March 2020

AbstractEmployment rates for autistic individuals are poor, even compared to those from other disability groups. Internationally, there remains limited understanding of the factors influencing employment across the stages of preparing for, gaining, and maintaining employment. This is the third in a series of studies conducted as part of an International Society for Autism Research (INSAR) policy brief intended to improve employment outcomes for autistic individuals. A multi‐informant international survey with five key stakeholder groups, including autistic individuals, their families, employers, service providers, and researchers, was undertaken in Australia, Sweden, and the United States to understand the facilitators and barriers to employment for autistic adults. A total of 687 individuals participated, including autistic individuals (n = 246), family members (n = 233), employers (n = 35), clinicians/service providers (n = 123), and researchers (n = 50). Perceptions of the facilitators and barriers to employment differed significantly across both key stakeholder groups and countries, however, ensuring a good job match and focusing on strengths were identified by all groups as important for success. Key barriers to employment included stigma, a lack of understanding of autism spectrum disorder (ASD) and communication difficulties. Results suggest that a holistic approach to employment for autistic individuals is required, aimed at facilitating communication between key stakeholders, addressing attitudes and understanding of ASD in the workplace, using strength‐based approaches and providing early work experience. Autism Res 2020, 13: 1195‐1214. © 2020 International Society for Autism Research, Wiley Periodicals, Inc.Lay SummaryAutistic individuals experience significant difficulty getting and keeping a job. This article presents a survey study involving autistic individuals, their families, employers, service providers and researchers in Australia, Sweden, and the United States to understand their perspectives on the factors that support or act as barriers to employment. While perspectives varied across key stakeholders, strategies such as using a holistic approach, targeting workplace attitudes and understanding, focusing on strengths, and providing early work experience are important for success.
A Cyclin D1‐Specific Single‐Chain Variable Fragment Antibody that Inhibits HepG2 Cell Growth and Proliferation
Authors: Wu Yan , Tang Weiwei , Cao Yuhua , Jiang Dazhi , Zhao Liangzhong , Zhao Jialiang , Zhang Ying , Li Chengjuan , Cheng Cheng , Wang Shuai , Yang Fang , Zhu Xun , Li Guiying 
Published: March 2020

AbstractCyclin D1 is a key regulatory factor of the G1 to S transition during cell cycle progression. Aberrant cyclin D gene amplification and abnormal protein expression have been linked to hepatocellular carcinoma (HCC) tumorigenesis. Intrabodies, effective anticancer therapies that specifically inhibit target protein function within all intracellular compartments, may block cyclin D1 function. Here, a single‐chain variable fragment (scFv) antibody against cyclin D1 (ADκ) selected from a human semi‐synthetic phage display scFv library is expressed in Escherichia coli as soluble ADκ. Purified ADκ specifically binds to recombinant and endogenous cyclin D1 with high affinity. To enable blocking of intracellular cyclin D1 activity, an endoplasmic reticulum (ER) retention signal sequence is added to the ADκ sequence to encode anti‐cyclin D1 intrabody ER‐ADκ. Transfection of HepG2 cells with expression vector encoding ER‐ADκ elicited intracellular ER‐ADκ expression leading to cyclin D1 binding, significant G1 phase arrest, and apoptosis that are mechanistically tied to decreased intracellular phosphorylated retinoblastoma protein (Rb) levels. Meanwhile, ER‐ADκ dramatically inhibited subcutaneous human HCC xenografts growth in nude mice in vivo after injection of tumors with expression vector encoding ER‐ADκ. These results demonstrate the potential of intrabody‐based cyclin D1 targeting therapy as a promising treatment for HCC.
Multi‐Omics Reveals Impact of Cysteine Feed Concentration and Resulting Redox Imbalance on Cellular Energy Metabolism and Specific Productivity in CHO Cell Bioprocessing
Authors: Ali Amr S. , Chen Rachel , Raju Ravali , Kshirsagar Rashmi , Gilbert Alan , Zang Li , Karger Barry L. , Ivanov Alexander R. 
Published: March 2020

AbstractChinese hamster ovary (CHO) cells are currently the primary host cell lines used in biotherapeutic manufacturing of monoclonal antibodies (mAbs) and other biopharmaceuticals. Cellular energy metabolism and endoplasmic reticulum (ER) stress are known to greatly impact cell growth, viability, and specific productivity of a biotherapeutic; but the molecular mechanisms are not fully understood. The authors previously employed multi‐omics profiling to investigate the impact of a reduction in cysteine (Cys) feed concentration in a fed‐batch process and found that disruption of the redox balance led to a substantial decline in cell viability and titer. Here, the multi‐omics findings are expanded, and the impact redox imbalance has on ER stress, mitochondrial homeostasis, and lipid metabolism is explored. The reduced Cys feed activates the amino acid response (AAR), increases mitochondrial stress, and initiates gluconeogenesis. Multi‐omics analysis reveals that together, ER stress and AAR signaling shift the cellular energy metabolism to rely primarily on anaplerotic reactions, consuming amino acids and producing lactate, to maintain energy generation. Furthermore, the pathways are demonstrated in which this shift in metabolism leads to a substantial decline in specific productivity and altered mAb glycosylation. Through this work, meaningful bioprocess markers and targets for genetic engineering are identified.
Systems metabolic engineering of Bacillus subtilis for efficient biosynthesis of 5‐methyltetrahydrofolate
Authors: Yang Han , Liu Yanfeng , Li Jianghua , Liu Long , Du Guocheng , Chen Jian 
Published: March 2020

Abstract5‐Methyltetrahydrofolate (5‐MTHF) is the major form of folate in human plasma and is the only folate form that can penetrate the blood–brain barrier. It has been widely used for the prevention and treatment of various diseases. It is mainly produced by chemical synthesis. However, the low production rate cannot meet the increasing demand. In addition, chemical synthesis is potentially detrimental to the environment. Despite various microorganisms synthetizing 5‐MTHF, an efficient 5‐MTHF bioproduction approach is lacking because of the tight regulation of the 5‐MTHF pathway and limited metabolic flux toward the folic acid pathway. In this study, the 5‐MTHF synthetic pathway in Bacillus subtilis was systematically engineered to realize 5‐MTHF accumulation and further improve 5‐MTHF production. Specifically, the 5‐MTHF synthesis pathway with dihydrofolate (DHF) as the precursor was strengthened to shift the metabolic flux to 5‐MTHF biosynthesis by replacing the native yitJ gene with Escherichia coli metF, knockout of purU, and overexpressing dfrA. The intracellular level of 5‐MTHF increased 26.4‐fold, reaching 271.64 µg/L. Next, the 5‐MTHF precursor supply pathway was strengthened by co‐overexpression of folC, pabB, folE, and yciA. This resulted in a 93.2‐fold improvement of the 5‐MTHF titer, which reached 960.27 µg/L. Finally, the clustered regularly interspaced short palindromic repeats interference system was used to identify key genes in the competitive and catabolic pathways for repression to further shift the metabolic flux toward 5‐MTHF biosynthesis. The repression of genes thyA (existing in the purine metabolic pathway), pheA (existing in the competitive metabolic pathway), trpE (existing in the competitive metabolic pathway), and panB (existing in the pantoate synthesis pathway) significantly increased the titer of 5‐MTHF. By repressing the pheA gene, the 5‐MTHF titer reached 1.58 mg/L, which was 153.8‐fold that of the wild‐type strain of B. subtilis 168. Through medium optimization, the 5‐MTHF titer reached 1.78 mg/L, which was currently the highest titer of 5‐MTHF in B. subtilis. Apart from the highest titer of 5‐MTHF, the highest titer of total folates including 5‐MTHF, 5‐FTHF, folic acid, and THF could reach 3.31 mg/L, which was 8.5‐fold that in B. subtilis. To the best of our knowledge, the 5‐MTHF and total folate titers reported here are the highest using a Generally regarded as safe (GRAS) bacterium as the production host. Overall, this study provides a good starting point for further metabolic engineering to achieve efficient biosynthesis of 5‐MTHF by GRAS bacteria.
Immobilization of horseradish peroxidase onto Purolite® A109 and its anthraquinone dye biodegradation and detoxification potential
Authors: Šekuljica Nataša Ž. , Jovanović Jelena R. , Jakovetić Tanasković Sonja M. , Ognjanović Nevena D. , Gazikalović Ivana V. , Knežević‐Jugović Zorica D. , Mijin Dušan Ž. 
Published: March 2020

AbstractHorseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite® A109 with a special focus on enzymatic decolorization and toxicity of artificially colored wastewater. The immobilized preparations with an activity of 156.21 ± 1.41 U g−1 and 85.71 ± 1.62 U g−1 after the HRP adsorption and covalent immobilization, respectively, were obtained. Stability and reusability of the immobilized preparations were also evaluated. A noteworthy decolorization level (~90%) with immobilized HRP was achieved. Phytotoxicity testing using Mung bean seeds and acute toxicity assay with Artemia salina has confirmed the applicability of the obtained immobilized preparation in industrial wastewater plants for the treatment of colored wastewater.
Efficacy and safety of anlotinib, a multikinase angiogenesis inhibitor, in combination with epirubicin in preclinical models of soft tissue sarcoma
Authors: Wang Zhi‐Ming , Zhang Shi‐Long , Yang Hua , Zhuang Rong‐Yuan , Guo Xi , Tong Han‐Xing , Zhang Yong , Lu Wei‐Qi , Zhou Yu‐Hong 
Published: March 2020

AbstractBackgroundAnlotinib is a novel, orally administered, multitarget receptor tyrosine kinase inhibitor. It functions by inhibiting tumor angiogenesis and proliferative signaling pathways. In this study, we aimed to investigate the efficacy and safety of anlotinib plus epirubicin in a sarcoma patient‐derived xenografts (PDX) model.MethodsWe firstly established a PDX model using fresh tumor tissues that were surgically removed from a patient diagnosed with malignant fibrous histiocytoma. Thirty‐six PDX models were divided into six groups and treated with anlotinib alone (low‐dose, 1.5 or high‐dose, 3.0 mg/kg/day, oral gavage), or with anlotinib plus epirubicin (3.0 mg/kg/once weekly, i.p.) when the tumors grew to 150‐200 mm3. After 5 weeks of treatment, the mice were sacrificed, and the tumors were measured by weight and processed for IHC and H&E staining. IHC staining was performed to detect CD31, EGFR, MVD, and Ki‐67 on paraffin sections. H&E stainings were performed to examine the microcosmic changes that occurred in the tumor tissues and myocardium, respectively.ResultsAfter 5 weeks, treatment with anlotinib or epirubicin alone significantly inhibited tumor growth in the sarcoma PDX model compared with the vehicle control. Tumor volume in the high‐dose anlotinib group was significantly smaller than the low‐dose anlotinib group (P < .001). Combined high‐dose anlotinib and epirubicin treatment resulted in the most pronounced tumor inhibition. In the groups treated with the anlotinib‐containing regimen, the expression levels of CD31, EGFR, MVD, and Ki‐67 were significantly low. The weight in each group had no statistical differences; the same applied to the hepatic function, cardiac function, and toxicity.ConclusionsHigh‐dose anlotinib combined with epirubicin was an effective and safe therapy for STS.
Identification of aberrantly methylated differentially expressed genes and associated pathways in endometrial cancer using integrated bioinformatic analysis
Authors: Liu JinHui , Wan YiCong , Li Siyue , Qiu HuaiDe , Jiang Yi , Ma Xiaoling , Zhou ShuLin , Cheng WenJun 
Published: March 2020

AbstractEndometrial cancer (EC) is a fatal female reproductive tumor. Bioinformatic tools are increasingly developed to screen out molecular targets related to EC. In this study, GSE17025 and GSE40032 were obtained from Gene Expression Omnibus (GEO). “limma” package and Venn diagram tool were used to identify hub genes. FunRich was used for functional analysis. Retrieval of Interacting Genes Database (STRING) was used to analyze protein‐protein interaction (PPI) complex. Cancer Genome Atlas (TCGA), GEPIA, immunohistochemistry staining, and ROC curve analysis were carried out for validation. Univariate and multivariate regression analyses were performed to predict the risk score. Compound muscle action potential (CMap) was used to find potential drugs. GSEA was also done. We retrieved seven oncogenes which were upregulated and hypomethylated and 12 tumor suppressor genes (TSGs) which were downregulated and hypermethylated. The upregulated and hypomethylated genes were strikingly enriched in term “immune response” while the downregulated and hypermethylated genes were mainly focused on term “aromatic compound catabolic process.” TCGA and GEPIA were used to screen out EDNRB, CDO1, NDN, PLCD1, ROR2, ESPL1, PRAME, and PTTG1. Among them, ESPL1 and ROR2 were identified by Cox regression analysis and were used to construct prognostic risk model. The result showed that ESPL1 was a negative independent prognostic factor. Cmap identified aminoglutethimide, luteolin, sulfadimethoxine, and maprotiline had correlation with EC. GSEA results showed that “hedgehog signaling pathway” was enriched. This research inferred potential aberrantly methylated DEGs and dysregulated pathways may participate in EC development and firstly reported eight hub genes, including EDNRB, CDO1, NDN, PLCD1, ROR2, ESPL1, PRAME, and PTTG1 that could be used to predict EC prognosis. Aminoglutethimide and luteolin may be used to fight against EC.
DPP‐4 Cleaves α/β‐Peptide Bonds: Substrate Specificity and Half‐Lives
Authors: Turalić Amila , Đeđibegović Jasmina , Hegedüs Zsófia , Martinek Tamás A. 
Published: March 2020

AbstractThe incorporation of β‐amino acids into a peptide sequence has gained particular attention as β‐ and α/β‐peptides have shown remarkable proteolytic stability, even after a single homologation at the scissile bond. Several peptidases have been shown to cleave such bonds with high specificity but at a much slower rate compared to α‐peptide bonds. In this study, a series of analogs of dipeptidyl peptidase‐4 (DPP‐4) substrate inhibitors were synthesized in order to investigate whether β‐amino acid homologation at the scissile bond could be a valid approach to improving peptide stability towards DPP‐4 degradation. DPP‐4 cleaved the α/β‐peptide bond after the N‐terminal penultimate Pro with a broad specificity and retained full activity regardless of the β3‐amino acid side chain and peptide length. Significantly improved half‐lives were observed for β3Ile‐containing peptides. Replacing the penultimate Pro with a conformationally constrained Pro mimetic led to proteolytic resistance. DPP‐4 cleavage of α/β‐peptide bonds with a broad promiscuity represents a new insight into the stability of peptide analogs containing β‐amino acids as such analogs were thought to be stable towards enzymatic degradation.