bluuebunny/crossref_metadata_2025_split huggingface dataset:
IMAGOLOGIA ROBÓTICA: robôs e inteligência artificial nas narrativas para crianças e jovens
Authors: D'Alte Pedro Gabriel 
Published: January 2020

As narrativas para a infância e juventude absorvem os discursos sobre a inteligência artificial e, por meio de processos literários abeirados ora de um certo mimetismo ora da mais virtuosa reconfiguração da realidade, apresentam cenários que convidam o leitor, desde tenra idade, a repensar um mundo permeado pelo digital. As narrativas escolhidas para o presente exercício intelectual intentam demonstrar um caleidoscópio de imagens variado e que dê conta de diferentes perspetivas face à inteligência artificial promovendo, desta forma, um exercício crítico e exploratório sobre a mesma.

PALAVRAS-CHAVE: Literatura para crianças e jovens; Inteligência artificial; Imagologia robótica; robôs na literatura para a infância.
ABSTRACTThe narratives for childhood and youth absorb the discourses about artificial intelligence and, through the use of literary processes either close to a certain mimicry or to the most virtuous reconfiguration of reality, present scenarios that invite the reader, since an early age, to rethink a world permeated by digital. The narratives chosen for the present intellectual exercise attempt to demonstrate a kaleidoscope of images and to account for different perspectives in relation to artificial intelligence, thus promoting a critical and exploratory exercise on this matter.

KEYWORDS: Children’s literature; Artificial intelligence; Robotic imagery; Robots in children’s literature.

RESUMEN
Las narrativas para la infancia y la juventud absorben discursos sobre inteligencia artificial y, a través de procesos literarios bien conocidos, a veces de una cierta imitación, a veces de la reconfiguración más virtuosa de la realidad, escenarios actuales que invitan al lector, desde una edad temprana, a repensar un mundo. impregnado por digital. Las narraciones elegidas para el presente ejercicio intelectual pretenden demostrar un caleidoscopio de imágenes variadas que ofrece diferentes perspectivas sobre la inteligencia artificial, promoviendo así un ejercicio crítico y exploratorio sobre el mismo.

PALABRAS CLAVE: Literatura para niños y jóvenes; Inteligencia artificial; Imagen robótica; robots en literatura para la infancia.
The Effect of Casing-Pipe Roughness on Cement-Plug Integrity
Authors: Corina Anisa Noor , Opedal Nils , Vrålstad Torbjørn , Skorpa Ragnhild , Sangesland Sigbjørn 
Published: January 2020

Summary
In permanently abandoned wells, cement plugs serve as a well-barrier element that is essential for providing long-term zonal isolation. Poorly plugged wells might provide leakage pathways that pose risks to the surrounding environment. It is well-known that the final quality of set cement is influenced by the cement placement, but the influence of downhole-pipe roughness to the hydraulic sealing of a cement plug has not been specifically investigated.
This paper presents an experimental study assessing the cement-plug sealing of neat- and silica-cement systems placed in pipes with three different levels of surface roughness. The experimental test uses a small-scale laboratory setup, consisting of a test cell filled with a cement plug, which can simulate realistic wellbore curing and operating conditions. By subjecting the cement plug to gas differential pressure, the setup detects any gas leak through the cement plug and measures the leak rate. As a complement to the experimental study, a computational-fluid-dynamics (CFD) simulation is conducted to study fluid flow around the cement plug.
Experimental tests detect an instant gas leak in all samples at low differential pressure (&lt;0.01 bar) through leak sources at the cement/steel interface, presumed to be microannuli. Furthermore, experimental results discover that placing cement plugs in rough-surface pipes could reduce the gas-leak rate, and the most significant reduction was found from samples cured in the roughest pipe. The CFD simulation results, along with pressure-buildup-curve observation, show that the leak path of samples placed in rough-surface pipes tends to be tortuous and less connected, thereby adding resistance for gas to flow.
Identification and functional characterization of NbMLP28, a novel MLP-like protein 28 enhancing Potato virus Y resistance in Nicotiana benthamiana
Authors: Song Liyun , Wang Jie , Jia Haiyan , Kamran Ali , Qin Yuanxia , Liu Yingjie , Hao Kaiqiang , Han Fei , Zhang Chaoqun , Li Bin , Li Yongliang , Shen Lili , Wang Fenglong , Wu Yuanhua , Yang Jinguang 
Published: January 2020

Abstract

Background: Major latex proteins (MLPs) belong to the MLP subfamily in Bet v 1 protein family and respond to both biotic and abiotic stresses, which play critical roles in plant disease resistance. As the type species of widely distributed and economically devastating Potyvirus, Potato virus Y (PVY) is one of the major constraints to important crop plants including tobacco ( Nicotiana benthamiana ) worldwide. Despite the great losses owing to PVY infection in tobacco, there is no previous study investigating the potential role of MLPs in developing resistance to viral infection. Results: In this study, for the first time we have identified and functionally analyzed the MLP-like protein 28 from N. benthamiana , denoted as NbMLP28 and investigated its role in conferring resistance to N. benthamiana against PVY infection. NbMLP28 was localized to the plasmalemma and nucleus, with the highest level in the root. NbMLP28 gene was hypothesized to be triggered by PVY infection and was highly expressed in jasmonic acid (JA) signaling pathway. Further validation was achieved through silencing of NbMLP28 through virus-induced gene silencing (VIGS) that rendered N. benthamiana plants more vulnerable to PVY infection, contrary to overexpression that enhanced resistance. Conclusions: Taken together, this is the first study describing the role of NbMLP28 in tobacco against PVY infection and provide a pivotal point towards obtaining pathogen-resistant tobacco varieties through constructing new candidate genes of MLP subfamily.
Metagenomics insight into microbial dynamics in field-scale remediation of crude oil-polluted soil
Authors: Chikere Chioma Blaise , Tekere Memory , Adeleke Rasheed 
Published: January 2020

Abstract
Background: The frequency of crude oil pollution has been on the increase following increased exploration, exploitation and production of energy from fossil fuel. Bioremediation has been shown to be eco-friendly and cost-effective method of oil spill remediation. In the Niger Delta, Landfarming has been the most used technique. The aim of this research was to employ metagenomic techniques to understand microbial dynamics during field-scale remediation in the Niger Delta in order to improve and reduce the time of remediation. Results: The surface (0.0 – 0.5m) sample had an extractable TPH value of 6231 mg/kg. The subsurface samples from 1m, 1.5m and 2.0m depths had extractable TPH concentration of 4836 mg/kg, 9112 mg/kg and 7273 mk/kg respectively. Proteobacteria dominated the soil microbial profile in all the samples studied as it made up at least 50% of each sample and mostly comprised of the class Alphaproteobacteria with variation only on day 18 and 36 which was mostly dominated by the class Gammaproteobacteria and Betaproteobacteria. Alpha diversity analysis revealed the presence of crude oil in the soil reduced microbial diversity. Principal coordinate analysis showed the microbial structure continually changed following changes in the chemical composition of the soil. Mycobacterium, Burkholderia, Rhodoplanes, Methylobacterium and Bacillus were the core OTUs detected during the period of remediation. Significant variation in pathway abundance particularly pathways for propanoate degradation, benzoate degradation, naphthalene degradation, fatty acid metabolism, polycyclic aromatic hydrocarbon degradation and degradation of xenobiotics were observed when the unpolluted soil was compared to the samples obtained during remediation. Conclusions: The findings from this study will greatly advance an already preferred landfarming oil spill recovery technique in the Niger Delta.
Developing a routine lab test for absolute quantification of Her2 in Formalin Fixed Paraffin Embedded (FFPE) breast cancer tissues using Quantitative Dot Blot (QDB) method
Authors: Yu Guohua , Zhang Wenfeng , Zhang Yunyun , Lv Jiahong , Wu Shishou , Sui Xiaolong , Zhang Jiandi , Tang Fangrong 
Published: January 2020

Abstract
Background: Developing immunoassay for Her2 assessment suitable for Formalin Fixed Paraffin Embedded (FFPE) samples promises improved objectivity, consistency and accuracy in daily clinical practice. Yet, this effort is hindered by lacking available technique. The feasibility of Quantitative Dot Blot (QDB) method to meet this demand was evaluated in this study. Methods: QDB-based Immunoassay was developed for quantitative measurement of Her2 protein levels in this retrospective study using clinically validated antibody for immunohistochemistry (IHC antibody). Total protein lysates were extracted from 2X15 um FFPE slices collected from 332 breast cancer patients sequentially and non-selectively from local hospital. The absolutely quantitated Her2 levels in these samples were compared with the results from methods of Immunohistochemistry (IHC) and Fluorescence in situ hybridization (FISH) analyses using Receiver Operative Characteristics (ROC) analysis. Results: Her2 levels measured with two IHC antibodies of EP3 and 4B5 were strongly correlated (r=0.963, p=0.0000). We also achieved area under the curve (AUC) at 0.9753±0.01026 (95% CI: 0.9551 to 0.9954, p<0.0001, n=322) using provided results from IHC and FISH analyses, with 99.6% concordance with IHC and 88.1% with FISH at optimized cutoff of 0.261 nmole/g. The concordance rate with FISH analysis was improved to 94.1% when disagreed samples were re-analyzed by third party independently. Conclusions: We introduced the first immunoassay for absolute quantitation of protein biomarker levels in FFPE samples. This method fits well with daily clinical practice with its objectivity, simplicity and consistency, especially for local hospitals and hospitals in developing countries. The quantitatively measured Her2 protein levels in FFPE samples also allow statistically analysis at population level to meet the need of precision medicine. .
Epileptic seizure discharges in patients after open chamber cardiac surgery – A prospective prevalence study using continuous electroencephalography
Authors: Tschernatsch Marlene , Juenemann Martin , Alhaidar Fouad , Shazly Jasmin El , Butz Marius , Meyer Marco , Gerriets Tibo , Schoenburg Markus , Schramm Patrick 
Published: January 2020

Abstract
Background Patients undergoing cardiac surgery often develop delirium and postoperative cognitive deficits (POCD), which lead to a higher postoperative morbidity, mortality and a reduced quality of life. Retrospective studies show a higher incidence of delirium, stroke and mortality in patients with convulsive and non-convulsive seizures after cardiac surgery. However, these studies do not systematically detect subclinical seizures in the early postoperative period; so, the incidence of seizures after cardiac surgery remains speculative. To investigate the real frequency of seizures, we conducted this prospective pilot study on patients with open-chamber cardiac surgery using continuous electroencephalography (EEG) monitoring utilizing widely distributed electrodes and 10-channel registration. The main objective of the study is to determine the prevalence of seizure-specific patterns in EEG after elective open-chamber cardiac surgery. Methods The prospective, blinded, monocentric, observational study investigated patients scheduled for elective open-chamber aortic or mitral valve reconstruction or replacement. The anaesthetic, surgical and postoperative treatment was standardized and not influenced by the presented observation. After surgery, all patients arrived at the ICU, and EEG monitoring started within the first hour after admission to the ICU (10 channel registration: Fp1,Fp2,C3,C4,P3,P4,T3,T4,Fz,Cz). EEG recording was continuously performed for up to 24 hours, and the EEG results were independently analysed with a focus on epileptic discharges and seizure activity by two blinded EEG board-certified physicians Results One hundred patients were included, 76% of whom underwent aortic valve replacement, 24% of whom endured mitral valve reconstruction or replacement. Early postoperative EEG recording lasted 12.9±7.2 hours. Epileptic EEG patterns were present in 38% of patients and seizure-specific EEG patterns were present in 22% of patients. Main EEG activity at the beginning of recording was suppressed or showed a burst-suppression pattern, and at the end of recording, all patients had an alpha/theta-rhythm. Conclusion This pilot study reveals a surprisingly high prevalence of seizure-specific EEG patterns (22%) in patients undergoing open-chamber cardiac surgery. As seizures potentially induce delirium and POCD, this finding is a relevant phenomenon in the post-cardiac surgery population, representing a promising target for the treatment and prevention of postoperative delirium and POCD.
Potentially Inappropriate Medications Use among Older Adults with Comorbid Diabetes and Hypertension
Authors: Alwhaibi Monira 
Published: January 2020

Abstract
Background Potentially inappropriate medications (PIMs) are medications that should be avoided among older adults due to their risk which offsets their benefit. The objective of this study is to estimate the prevalence of PIMs use and to evaluate its associated factors among older adults with comorbid diabetes and hypertension using the 2019 Beers criteria.Methods A cross-sectional retrospective study design was used. This study included 1,853 older adults (age ≥ 65 years) with comorbid diabetes and hypertension visited an ambulatory care setting in a large hospital in the central region of Saudi Arabia. The primary outcome was the prevalence of PIMs use based on the updated 2019 American Geriatric Society (AGS) Beers Criteria. The secondary outcome was the factors associated with the presence of PIMs use (use of one or more PIMs) by referencing the Beers Criteria list.Results Almost one out of two individuals had PIMs use with the average number of medication taken was seven; where 40.3% of the older adults taken one PIMs, and about 16% were using two or more PIMs. The most commonly prescribed PIMs were the use of gastrointestinal and endocrine medications. High risk of PIMs use was among those with ischemic heart disease and anxiety comorbidities and those using multiple medications (i.e., polypharmacy).Conclusions Given higher PIMs use among older adults with diabetes and hypertension comorbidities, tailored strategies and interventions to minimize the PIMs use in this population are warranted. There is a need for greater vigilance when managing patients with comorbid conditions to avoid the use of inappropriate medications.
The relationship of destinezite to the acid sulfate alteration at the El Laco magnetite deposit, Chile
Authors: Velasco Francisco , de la Pinta Noelia , Tornos Fernando , Briezewski Thomas , Larrañaga Aitor 
Published: January 2020

Abstract
Destinezite, ideally Fe23+(PO4)(SO4)(OH)·6(H2O), is found as nodular lumps in hematite-rich epiclastic sediments accumulated in small crater lakes on the slopes of El Laco volcano. These lumps are almost entirely dominated by fine-grained destinezite replacing earlier lipscombite, and associated with gray hematite. The crystal structure of destinezite has been re-examined to test for possible differences with respect to the earthy and poorly crystalline destinezite that forms by weathering in cave soils and mine waste dumps. The structural refinements confirm that the differences are minor. The El Laco destinezite was refined in space group P1 with a = 9.5828(2), b = 9.7440(3), c = 7.3302(3) Å, and α = 98.796(3)°, β = 107.985(3)°, γ = 63.898(2)°, V = 584.50(4) Å3, and Z = 1. We measured by calorimetry the enthalpy and derived the entropy and the Gibbs free energy of formation of destine-zite (–4051.7 ± 4.3, –1518.5 ± 20.0, and –3598.9 ± 7.1 kJ/mol, respectively). This has allowed us to estimate the equilibrium constant in the temperature range 0–300 °C (log K = –27.97 ± 1.1). We can predict that destinezite coexists with hematite over the range of conditions that typically encompass the steam-heated hydrothermal environments recognized at El Laco. The presence of destinezite along with hematite and the occurrence of alunite, jarosite, and variscite in the system provide evidence of intense hydrothermal alteration during the extrusion of Fe-rich melts at El Laco volcano. The mineral assemblage and the available geochemical data suggest that destinezite formed in hydrothermal conditions by “maturation” of an immiscible Fe–P-rich melt. An unknown Fe–P–O phase produced by this melt was first replaced by lipscombite and later, owing to intense sulfidation, by destinezite. This replacement took place in the vadose zone above the paleowater table by relatively cool (&lt;150 °C) groundwaters acidified by oxidation of magmatic SO2 to aqueous sulfate in a steam-heated system. Our model precludes other modes of formation, such as devitrification of hypothetical Fe–P–S–O-rich melts ejected from the volcano and supergene crystallization.
REE redistributions during granite weathering: Implications for Ce anomaly as a proxy for paleoredox states
Authors: Ichimura Koji , Sanematsu Kenzo , Kon Yoshiaki , Takagi Tetsuichi , Murakami Takashi 
Published: January 2020

Abstract
Different responses of Ce to the redox state from those of the other light rare earth elements (LREEs) can be used to understand paleoredox states. To establish the possibility of using the Ce anomaly as a proxy for paleo-environments, we examined the mineralogical and chemical characteristics of bulk samples and REE-bearing minerals of a modern weathering profile developed on granite, by X-ray fluorescence analysis, laser-ablation inductively coupled plasma mass spectrometry, field emission electron microprobe analysis, field emission scanning electron microscopy, and X-ray diffractometry. Bulk samples showed no significant Ce-anomalies except for the topmost layer that had a positive Ce-anomaly reflecting significant loss of LREEs except for Ce. Allanite-(Ce), primary REE-bearing mineral, contributed to ~100% of La, Ce, Pr, and Nd in the parent rock, and gradually decreased in amount toward the topmost layer. Secondary cerianite-(Ce) [Ce(IV)O2] was observed in the weathering profile, especially at shallower depths. Secondary rhabdophane-(La), -(Ce), -(Nd), and -(Y) were also observed in the weathering profile but in less amounts in the topmost layer. The occurrences of rhabdophane-(La) and -(Nd) in contact with halloysite, a secondary clay mineral, suggest probable adsorption of REEs onto halloysite prior to their formation. Similar formation mechanisms are likely for rhabdophane-(Ce) that commonly occurred in grain boundaries and was usually formed in contact with halloysite. Rhabdophane-(Y) occurred in association with fluorapatite. The ratios of La, Pr, and Nd of rhabdophane-(La), -(Ce), and -(Nd) were similar to that of allanite-(Ce), suggesting that these LREEs are inherited from allanite-(Ce) and behave similarly before the formation of rhabdophane. Different negative Ce-anomaly values of rhabdophane [i.e., ~0.03–0.34 for rhabdophane-(La), -(Nd), and -(Y), and ~0.6 for rhabdophane-(Ce)] can result from a difference in intensity of the formation of cerianite-(Ce) prior to the precipitation of rhabdophane. We have classified LREE redistributions in both secondary minerals and bulk weathered samples during oxic weathering and suggested that Ce anomaly can provide useful information on anoxic weathering and thus atmospheric oxygen evolution in the Precambrian if Ce anomalies of both bulk samples and secondary REE-bearing minerals are determined.
In-situ measurements of magmatic volatile elements, F, S, and Cl, by electron microprobe, secondary ion mass spectrometry, and heavy ion elastic recoil detection analysis
Authors: Rose-Koga Estelle F. , Koga Kenneth T. , Devidal Jean-Luc , Shimizu Nobumichi , Le Voyer Marion , Dalou Celia , Döbeli Max 
Published: January 2020

AbstractElectron probe and ion probe are the two most used instruments for in situ analysis of halogens in geological materials. The comparison of these two methods on widely distributed glass standards (example: MPI-DING glasses, Jochum et al., G-cubed, 2006) provides a basis for establishing laboratory method, independent geochemical data sets for these elements. We report analyses of F, S, and Cl concentrations in three geological glass samples (EPMA) and 10 referenced standards (EPMA and SIMS). Furthermore, F and Cl absolute abundances have been determined independently for three of the standards (KL2-G, ATHO-G, and KE12), via heavy ion elastic recoil detection analysis (HIERDA), to certify the accuracy of the cross-calibration EPMA-SIMS. The detection limits for EPMA are a 150 μg·g-1 for F, 20 μg·g-1 for S and Cl, and for SIMS &lt; 48 μg·g-1 for F, &lt; 3 μg·g-1 for S, and &lt;19 μg·g-1 for Cl. On SiO2-rich glass-standards, F and Cl measurements by HIERDA highlight a weak matrix effect during SIMS analysis of F and Cl. With the HIERDA independently measured value, we therefore propose an alternative calibration function to empirically correct this matrix effect on the SIMS measurements of F, S, and Cl.
Treatment with the Ketone Body D-β-hydroxybutyrate Attenuates Autophagy Activated by NMDA and Reduces Excitotoxic Neuronal Damage in the Rat Striatum In Vivo
Authors: Montiel Teresa , Montes-Ortega Luis A. , Flores-Yáñez Susana , Massieu Lourdes 
Published: January 2020

Background:
The ketone bodies (KB), β-hydroxybutyrate (BHB) and acetoacetate, have been proposed
for the treatment of acute and chronic neurological disorders, however, the molecular mechanisms involved
in KB protection are not well understood. KB can substitute for glucose and support mitochondrial metabolism
increasing cell survival. We have reported that the D-isomer of BHB (D-BHB) stimulates autophagic degradation
during glucose deprivation in cultured neurons increasing cell viability. Autophagy is a lysosomal degradation
process of damaged proteins and organelles activated during nutrient deprivation to obtain building blocks and
energy. However, impaired or excessive autophagy can contribute to neuronal death.

Objective:
The aim of the present study was to test whether D-BHB can preserve autophagic function in an in
vivo model of excitotoxic damage induced by the administration of the glutamate receptor agonist, N-methyl-Daspartate
(NMDA), in the rat striatum.

Methods:
D-BHB was administered through an intravenous injection followed by either an intraperitoneal injection
(i.v+i.p) or a continuous epidural infusion (i.v+pump), or through a continuous infusion of D-BHB alone.
Changes in the autophagy proteins ATG7, ATG5, BECLIN 1 (BECN1), LC3, Sequestrosome1/p62 (SQSTM1/
p62) and the lysosomal membrane protein LAMP2, were evaluated by immunoblot. The lesion volume was
measured in cresyl violet-stained brain sections.

Results:
Autophagy is activated early after NMDA injection but autophagic degradation is impaired due to the
cleavage of LAMP2. Twenty-four h after NMDA intrastriatal injection, the autophagic flux is re-established, but
LAMP2 cleavage is still observed. The administration of D-BHB through the i.v+pump protocol reduced the
content of autophagic proteins and the cleavage of LAMP2, suggesting decreased autophagosome formation and
lysosomal membrane preservation, improving autophagic degradation. D-BHB also reduced brain injury. The
i.v+i.p administration protocol and the infusion of D-BHB alone showed no effect on autophagy activation or
degradation.