bluuebunny/crossref_metadata_2025_split huggingface dataset:
The histone methyltransferase Setd8 alters the chromatin landscape and regulates the expression of key transcription factors during erythroid differentiation
Authors: Myers Jacquelyn A. , Couch Tyler , Murphy Zachary , Malik Jeffrey , Getman Michael , Steiner Laurie A. 
Published: March 2020

Abstract
Background
SETD8 is the sole methyltransferase capable of mono-methylating histone H4, lysine 20. SETD8 and H4K20me1 play a role in a number of essential biologic processes, including cell cycle progression, establishment of higher order chromatin structure, and transcriptional regulation. SETD8 is highly expressed in erythroid cells and erythroid deletion of Setd8 is embryonic lethal by embryonic day 11.5 (E11.5) due to profound anemia, suggesting that it has an erythroid-specific function. The function of SETD8 in the hemopoietic system is poorly understood. The goal of our study was to gain insights into the function of SETD8 during erythroid differentiation.

Results
We performed ATAC-seq (assay for transposase-accessible chromatin) on sorted populations of E10.5 Setd8 mutant and control erythroblasts. Accessibility profiles were integrated with expression changes and a mark of heterochromatin (H3K27me3) performed in wild-type E10.5 erythroblasts to further understand the role of SETD8 in erythropoiesis. Data integration identified regions of greater chromatin accessibility in Setd8 mutant cells that co-located with H3K27me3 in wild-type E10.5 erythroblasts suggesting that these regions, and their associated genes, are repressed during normal erythropoiesis. The majority of these more accessible regions were located in promoters and they frequently co-located with the NFY complex. Pathway analysis of genes identified through data integration revealed stemness-related pathways. Among those genes were multiple transcriptional regulators active in multipotent progenitors, but repressed during erythroid differentiation including Hhex, Hlx, and Gata2. Consistent with a role for SETD8 in erythroid specification, SETD8 expression is up-regulated upon erythroid commitment, and Setd8 disruption impairs erythroid colony forming ability.

Conclusion
Taken together, our results suggest that SETD8 is an important regulator of the chromatin landscape during erythroid differentiation, particularly at promoters. Our results also identify a novel role for Setd8 in the establishment of appropriate patterns of lineage-restricted gene expression during erythroid differentiation.
Relationship of Leishmania RNA Virus (LRV) and treatment failure in clinical isolates of Leishmania major
Authors: Abtahi Mohsen , Eslami Gilda , Cavallero Serena , Vakili Mahmood , Hosseini Saeedeh Sadat , Ahmadian Salman , Boozhmehrani Mohammad Javad , Khamesipour Ali 
Published: March 2020

AbstractObjectiveLeishmaniasis is caused by differentLeishmaniaspp. Treatment failure (TF) of cutaneous leishmaniasis (CL) is a serious issue that may be due to various reasons, previous studies suggested Leishmania RNA virus (LRV) as a potential cause of TF. Two variant groups of LRV1 and LRV2 are reported. In this study, the presence of LRV1/LRV2 was compared in TF with treatment response (TR) isolates ofL. major. Clinical isolates of 15 TF and 15 TR were collected from CL patients referred to the Health Centers of Isfahan. Genomic DNA was extracted to identifyLeishmaniaspp. using ITS1-PCR–RFLP. Identification of LRV1/LRV2 was performed using SYBR Green Real-Time PCR. The statistical analysis to test relationship between the treatment response with Glucantime and the presence of LRV were performed using SPSS 16.0 with Fisher’s Exact test.Pvalue of less than 0.05 was considered significant.ResultsITS1-PCR–RFLP results showed that every isolate was identified asL. major. The results showed no LRV1 in any of the samples but 7 TR isolates and 2 TF isolates showed positive for LRV2. Statistical analysis showed no significant difference between the presence of LRV2 and response to Glucantime (p-value = 0.1086). Therefore, other mechanisms might be responsible for TF.
Downregulation of circulating miR-320a and target gene prediction in patients with diabetic retinopathy
Authors: Prado Marcelle SanJuan Ganem , de Jesus Mirthz Lemos , de Goes Thaline Cunha , Mendonça Lucilla Silva Oliveira , Kaneto Carla Martins 
Published: March 2020

Abstract
Objective
To evaluate the expression of a set of miRNAs to identify differentially expressed miRNAs that might be considered reliable biomarkers on Diabetic Retinopathy (DR) blood samples.

Results
Expression levels of MiR-320a, MiR-342-3p, MiR-155, MiR-99a, MiR-29a and MiR-27b were analyzed in 60 healthy controls, 48 Diabetes Melitus (DM) without DR patients and 62 DR patients by qRT-PCR. MiR-320a was shown to be downregulated in the plasma of DR patients compared with DM patients without DR and healthy subjects. Target genes were predicted using miRWalk3.0, miR targeting data and target gene interaction data were imported to Cytoscape to visualize and merge networks and top ranked predicted genes were run through Ontology Genes to perform enrichment analysis on gene sets and classification system to identify biological processes and reactome pathways associated with DR. Highly scored target genes of miR-320a were categorized for various biological processes, including negative regulation of cell aging, negative regulation of cellular protein metabolic process and regulation of cellular response to stress that are critical to the development of DR. Our findings suggest that MiR-320a may have a role in the pathogenesis of DR and may represent novel biomarkers for this disease.
Fanconi anemia pathway as a prospective target for cancer intervention
Authors: Liu Wenjun , Palovcak Anna , Li Fang , Zafar Alyan , Yuan Fenghua , Zhang Yanbin 
Published: March 2020

AbstractFanconi anemia (FA) is a recessive genetic disorder caused by biallelic mutations in at least one of 22 FA genes. Beyond its pathological presentation of bone marrow failure and congenital abnormalities, FA is associated with chromosomal abnormality and genomic instability, and thus represents a genetic vulnerability for cancer predisposition. The cancer relevance of the FA pathway is further established with the pervasive occurrence of FA gene alterations in somatic cancers and observations of FA pathway activation-associated chemotherapy resistance. In this article we describe the role of the FA pathway in canonical interstrand crosslink (ICL) repair and possible contributions of FA gene alterations to cancer development. We also discuss the perspectives and potential of targeting the FA pathway for cancer intervention.
Zoom motion estimation for color and depth videos using depth information
Authors: Kwon Soon-kak , Lee Dong-seok 
Published: March 2020

AbstractIn this paper, two methods of zoom motion estimation for color and depth videos by using depth information are proposed. Color and depth videos are independently estimated for zoom motion. Zoom for color video is scaled by spatial domain, and depth video is scaled by both spatial and depth domains. For color video, instead of existing methods of zoom motion estimation that apply all of possible zoom ratios for a current block, the zoom ratio of the proposed method is determined as the ratio of the average depth values of the current and reference blocks. Then, the reference block is resized by multiplying the zoom ratio and the reference block is mapped to the current block. For depth video, the reference block is first scaled in the spatial direction by the same methodology used for color video and then scaled by a distance ratio from a camera to the objects. Compared to the conventional motion estimation method, the proposed method reduces MSE by up to about 30% for the color video and up to about 85% for the depth video.
On Copson’s inequalities for $0< p<1$
Authors: Gao Peng , Zhao HuaYu 
Published: March 2020

AbstractLet $(\lambda_{n})_{n \geq1}$(λn)n≥1 be a positive sequence and let $\varLambda_{n}=\sum^{n}_{i=1}\lambda_{i}$Λn=∑i=1nλi. We study the following Copson inequality for $0< p<1$0<p<1, $L>p$L>p: $$\begin{aligned} \sum^{\infty}_{n=1} \Biggl(\frac{1}{\varLambda_{n}} \sum^{\infty }_{k=n}\lambda_{k} x_{k} \Biggr)^{p} \geq \biggl( \frac{p}{L-p} \biggr)^{p} \sum^{\infty}_{n=1}x^{p}_{n}. \end{aligned}$$ ∑n=1∞(1Λn∑k=n∞λkxk)p≥(pL−p)p∑n=1∞xnp. We find conditions on $\lambda_{n}$λn such that the above inequality is valid with the constant being the best possible.
Spatial distribution and associated factors of health insurance coverage in Ethiopia: further analysis of Ethiopia demographic and health survey, 2016
Authors: Kebede Sewnet Adem , Liyew Alemneh Mekuriaw , Tesema Getayeneh Antehunegn , Agegnehu Chilot Desta , Teshale Achamyeleh Birhanu , Alem Adugnaw Zeleke , Yeshaw Yigizie 
Published: March 2020

AbstractBackgroundHealth insurance is one of the instruments to achieve universal health coverage. However, in Ethiopia, the coverage of health insurance is very low and varies from place to place as well. Therefore, exploring the spatial distribution of health insurance is important to prioritize and design targeted intervention programs in the country.MethodsA total of 16,583 reproductive age group women (15–49 years) were included in this study. The Bernoulli model was used by applying Kulldorff methods using the SaTScan software to analyse the purely spatial clusters of health insurance coverage. ArcGIS version 10.3 was used to visualize the distribution of health insurance coverage across the country. Mixed-effect logistic regression analysis was also used to identify predictors of health insurance coverage.ResultsHealth insurance coverage among women aged 15–49 years had spatial variations across the country (Moran’s I: 0.115,p < 0.001). Health insurance coverage in Amhara (p < 0.001) and Tigray (p < 0.001) National Regional States clustered spatially. Reading newspapers at least once a week (Adjusted Odds Ratio (AOR) = 1.78, 95% CI: (1.18–2.68))), 40–44 years of age (AOR = 2.14, 95% CI: (1.37–3.35)), clerical working mothers (AOR = 4.33, 95% CI: (2.50–7.49)), mothers’ with secondary school education (AOR = 1.77; 95% CI: (1.21–2.58)), mothers’ with higher school education (AOR = 2.62; 95% CI: (1.63–4.23)), having more than 5 family members (AOR = 1.25; 95% CI: (1.01–1.55)) and richest wealth quantile (AOR = 3.43, 95% CI: (1.96–6.01)) were predictors of health insurance coverage among reproductive age group women in Ethiopia.ConclusionHealth insurance coverage was very low in Ethiopia and had spatial variations across the country. The hot spot areas with low health insurance coverage need more coherent and harmonized action such as strengthening financial protection through national health packages, sharing experience from regions which have better health insurance coverage and using mass media to increase awareness and confidence of potentials in the systems, which may encourage them to enrol.
Probabilistic upscaling and aggregation of wind power forecasts
Authors: Henze Janosch , Siefert Malte , Bremicker-Trübelhorn Sascha , Asanalieva Nazgul , Sick Bernhard 
Published: March 2020

Abstract
Background
Wind power forecasts of the expected wind feed-in for the next hours or days are necessary to integrate the generated volatile wind energy into power systems. Most forecasting models predict in some sense the best value, but they ignore the other possible outcomes which may arise because of forecasting uncertainties. Probabilistic forecasts, on the other hand, also predict a distribution of possible outcomes with their respective probabilities that specific power values will occur and therefore have higher information content. In this work, we address two problems that hinder the introduction of probabilistic forecasts in practice: (1) no measurement data are available for some wind farms and (2) the flexible aggregation of probabilistic forecasts for changing wind farm portfolios.

Methods
We present an approach based on copulas that can solve both problems by modeling the spatial correlation structure between reference wind farms. By sampling from the resulting joint probability distribution, probability forecasts can be upscaled from reference wind farms to wind farms without power measurements. Furthermore, the results can be aggregated to probability forecasts of portfolios of arbitrary and changing size.

Results
We perform experiments by applying our procedure to three use cases. The results are quantitatively evaluated with different probabilistic scores. For single target wind farms, our approach is as good as a state-of-the-art reference even if no data are available for the wind farm under consideration. For portfolios, our approach also allows forecasts to be made if no data is available for some wind farms and also to aggregate flexible portfolios of changing sizes, which was not possible before.

Conclusion
Our work solves two problems that hindered the introduction of quantile probabilistic forecasts in the application. This work opens a pathway for many different applications, e.g., predictive grid securities with stochastic optimization, better marketing of renewable energies, or allow to compensate for forecast errors in various applications.
Isoniazid causes heart looping disorder in zebrafish embryos by the induction of oxidative stress
Authors: Ni Jie , Wang Hongye , Wei Xiyi , Shen Kangjie , Sha Yeqin , Dong Yuxiang , Shu Yimei , Wan Xiaojie , Cheng Jingwen , Wang Fang , Liu Yihai 
Published: March 2020

AbstractBackgroundThe cardiotoxicity of isoniazid on zebrafish embryos and its underlying mechanism is unclear.MethodsHere, we exposed zebrafish embryos at 4 h post-fertilization to different levels of isoniazid and recorded the morphology and number of malformed and dead embryos under the microscope.ResultsThe high concentration of isoniazid group showed more malformed and dead embryos than the low concentration of isoniazid group and control group. The morphology of the heart and its alteration were visualized using transgenic zebrafish (cmlc2: GFP) and confirmed by in situ hybridization. The negative effects of isoniazid on the developing heart were characterized by lower heart rate and more heart looping disorders. Mechanistically, PCR showed decreased expression of heart-specific transcription factors when exposed to isoniazid. Oxidative stress was induced by isoniazid in cardiomyocytes, mediated by decreased activities of catalase and superoxide dismutase, which were rescued by scavengers of reactive oxygen species.ConclusionIn conclusion, this study demonstrated that isoniazid led to heart looping disturbance by the downregulation of cardiac-specific transcription factors and induction of cardiomyocyte apoptosis.
Investigating the potential effects of selective histone deacetylase 6 inhibitor ACY1215 on infarct size in rats with cardiac ischemia-reperfusion injury
Authors: Lin Chao-Feng , Hsu Kai-Cheng , HuangFu Wei-Chun , Lin Tony Eight , Huang Han-Li , Pan Shiow-Lin 
Published: March 2020

Abstract
Background
Despite the fact that histone deacetylase (HDAC) inhibitors have been tested to treat various cardiovascular diseases, the effects of selective HDAC6 inhibitor ACY1215 on infarct size during cardiac ischemia-reperfusion (IR) injury still remain unknown. In the present study we aimed to investigate the effects of ACY1215 on infarct size in rats with cardiac IR injury, as well as to examine the association between HDAC6 inhibitors and the gene expression of hypoxia inducible factor-1α (HIF-1α), a key regulator of cellular responses to hypoxia.

Methods
By using computational analysis of high-throughput expression profiling dataset, the association between HDAC inhibitors (pan-HDAC inhibitors panobinostat and vorinostat, and HDAC6 inhibitor ISOX) and their effects on HIF-1α gene-expression were evaluated. The male Wistar rats treated with ligation of left coronary artery followed by reperfusion were used as a cardiac IR model. ACY1215 (50 mg/kg), pan-HDAC inhibitor MPT0E028 (25 mg/kg), and vehicle were intraperitoneally injected within 5 min before reperfusion. The infarct size in rat myocardium was determined by 2,3,5-triphenyltetrazolium chloride staining. The serum levels of transforming growth factor-β (TGF-β) and C-reactive protein (CRP) were also determined.

Results
The high-throughput gene expression assay showed that treatment of ISOX was associated with a more decreased gene expression of HIF-1α than that of panobinostat and vorinostat. Compared to control rats, ACY1215-treated rats had a smaller infarct size (49.75 ± 9.36% vs. 19.22 ± 1.70%, p < 0.05), while MPT0E028-treated rats had a similar infarct size to control rats. ACY-1215- and MPT0E028-treated rats had a trend in decreased serum TGF-β levels, but not statistically significant. ACY1215-treated rats also had higher serum CRP levels compared to control rats (641.6 μg/mL vs. 961.37 ± 64.94 μg/mL, p < 0.05).

Conclusions
Our research indicated that HDAC6 inhibition by ACY1215 might reduce infarct size in rats with cardiac IR injury possibly through modulating HIF-1α expression. TGF-β and CRP should be useful biomarkers to monitor the use of ACY1215 in cardiac IR injury.
Exploring the role of social media in collaborative learning the new domain of learning
Authors: Ansari Jamal Abdul Nasir , Khan Nawab Ali 
Published: March 2020

AbstractThis study is an attempt to examine the application and usefulness of social media and mobile devices in transferring the resources and interaction with academicians in higher education institutions across the boundary wall, a hitherto unexplained area of research. This empirical study is based on the survey of 360 students of a university in eastern India, cognising students’ perception on social media and mobile devices through collaborative learning, interactivity with peers, teachers and its significant impact on students’ academic performance. A latent variance-based structural equation model approach was followed for measurement and instrument validation. The study revealed that online social media used for collaborative learning had a significant impact on interactivity with peers, teachers and online knowledge sharing behaviour.Additionally, interactivity with teachers, peers, and online knowledge sharing behaviour has seen a significant impact on students’ engagement which consequently has a significant impact on students’ academic performance. Grounded to this finding, it would be valuable to mention that use of online social media for collaborative learning facilitate students to be more creative, dynamic and research-oriented. It is purely a domain of knowledge.
Omori-like slow decay (p < 1) of postseismic displacement rates following the 2011 Tohoku megathrust earthquake
Authors: Morikami Shunsuke , Mitsui Yuta 
Published: March 2020

AbstractWe infer the postseismic deformation mechanisms following the 2011 Tohoku megathrust earthquake via an analysis of onshore geodetic observations. We focus on the temporal decay characteristics of postseismic deformation using continuous time-series data at time scales that span many orders of magnitude by means of high-rate GNSS data. Our analysis indicates Omori-like power-law decay of the horizontal ground displacement rates, withp-value (0.69) that is significantly smaller than that of the aftershock occurrence (~ 1). This slow decay implies a (non-Maxwellian) viscoelastic relaxation mechanism other than afterslip since immediately after the mainshock, which is inferred using only onshore geodetic data. Spatial distribution of the Omori parameters implies that the postseismic deformation will continue over 100 years in a down-dip area of the northern part of the mainshock fault. The decay characteristics of vertical displacement rates are also almost Omori-like, but data deviation from the fitting line several 100 days after the mainshock might reflect the change of the dominant mechanism of the postseismic deformation. This multi-time scale geodetic approach will provide important constraints for future viscoelastic models of Earth’s interior.
Open-wedge high tibial osteotomy for spontaneous osteonecrosis of the medial tibial plateau shows excellent clinical outcomes
Authors: Goshima Kenichi , Sawaguchi Takeshi , Shigemoto Kenji , Iwai Shintaro , Fujita Kenji , Yamamuro Yuki 
Published: March 2020

AbstractPurposeThere have been few reports on medial tibial plateau osteonecrosis, and treatment options remain controversial. This study aimed to evaluate the clinical outcomes of open-wedge high tibial osteotomy (OWHTO) for osteonecrosis of the medal tibial plateau.MethodsPatients who underwent OWHTO for spontaneous osteonecrosis of the medial tibial plateau from November 2013 to September 2017 at our institution and followed up for at least 2 years after surgery were included in this study. Patients with history of alcohol abuse and corticosteroid therapy were excluded. Clinical evaluations, including the Japanese Orthopedic Association (JOA) score and the Oxford Knee Score (OKS), were measured preoperatively and at the final followup. Radiological evaluations included the weight-bearing line ratio (WBLR) and the lesion stage of the osteonecrosis according to Carpintero, Lotke, and the modified Ficat and Arlet classification. The area and size of the necrosis and the type of meniscus tear were also evaluated using preoperative magnetic resonance imaging (MRI). Additionally, cartilage regeneration was evaluated at plate removal.ResultsTwelve cases that underwent OWHTO for spontaneous osteonecrosis of the medial tibial plateau were enrolled. Eleven cases had isolated medial tibial osteonecrosis, and one case had both femoral and tibial osteonecrosis. The mean age was 59.6 ± 9.0 years, and the mean follow-up period was 41.8 ± 17.6 months.The WBLR significantly changed after OWHTO (24.0% ± 10.7% to 66.3% ± 6.7%, P < 0.001), and all clinical scores significantly improved after surgery: JOA score 63.3 ±12.3 to 95.0 ± 4.8, OKS 27.4 ± 7.8 to 42.6 ± 4.1, both 0.001. There were no adverse complications requiring additional surgery. The MRI findings revealed that all cases had meniscal lesions in addition to a necrotic lesion. Second-look arthroscopy was performed at plate removal in 11 cases, and cartilage regeneration was observed in 9/11 cases (81.8%).ConclusionsThis study’s results demonstrated that OWHTO is an effective procedure for spontaneous osteonecrosis of the medial tibial plateau with respect to subjective and objective clinical outcomes.
Staging of the estrous cycle and induction of estrus in experimental rodents: an update
Authors: Ajayi Ayodeji Folorunsho , Akhigbe Roland Eghoghosoa 
Published: March 2020

Abstract
Background
Determination of the phases of the estrous cycle and induction of estrus (heat) in experimental animals remains useful, especially in reproductive function research.

Main body of the abstract
This review provides a detailed description and discusses extensively the variations observed in different phases of the estrous cycle in laboratory animals using rats and mice as examples. It also illustrates how these phases can be determined and how to induce estrus ‘heat’ when required. The phases of the estrous cycle can be determined using various methods such as visual assessment, vaginal smear/cytology, histology of female reproductive organs (vagina, uterus and ovaries), vaginal wall impedance assessment and determination of urine biochemical parameters. Female animals can be artificially brought to estrus phase ‘heat’ to make them receptive to male counterparts.

Conclusion
Determination of the length and phases of the estrous cycle and induction of estrus are useful in teaching and research and evaluating the effects of drugs/chemicals on the reproductive functions.