bluuebunny/crossref_metadata_2025_split huggingface dataset:
A prolonged multispecies outbreak of IMP-6 carbapenemase-producing Enterobacterales due to horizontal transmission of the IncN plasmid
Authors: Yamagishi Takuya , Matsui Mari , Sekizuka Tsuyoshi , Ito Hiroaki , Fukusumi Munehisa , Uehira Tomoko , Tsubokura Miyuki , Ogawa Yoshihiko , Miyamoto Atsushi , Nakamori Shoji , Tawa Akio , Yoshimura Takahisa , Yoshida Hideki , Hirokawa Hidetetsu , Suzuki Satowa , Matsui Tamano , Shibayama Keigo , Kuroda Makoto , Oishi Kazunori 
Published: March 2020

AbstractA multispecies outbreak of IMP-6 carbapenemase-producing Enterobacterales (IMP-6-CPE) occurred at an acute care hospital in Japan. This study was conducted to understand the mechanisms of IMP-6-CPE transmission by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing and whole-genome sequencing (WGS), and identify risk factors for IMP-6-CPE acquisition in patients who underwent abdominal surgery. Between July 2013 and March 2014, 22 hospitalized patients infected or colonized with IMP-6-CPE (Escherichia coli [n = 8], Klebsiella oxytoca [n = 5], Enterobacter cloacae [n = 5], Klebsiella pneumoniae [n = 3] and Klebsiella aerogenes [n = 1]) were identified. There were diverse PFGE profiles and sequence types (STs) in most of the species except for K. oxytoca. All isolates of K. oxytoca belonged to ST29 with similar PFGE profiles, suggesting their clonal transmission. Plasmid analysis by WGS revealed that all 22 isolates but one shared a ca. 50-kb IncN plasmid backbone with blaIMP-6 suggesting interspecies gene transmission, and typing of plasmids explained epidemiological links among cases. A case-control study showed pancreatoduodenectomy, changing drains in fluoroscopy room, continuous peritoneal lavage and enteric fistula were associated with IMP-6-CPE acquisition among the patients. Plasmid analysis of isolates in an outbreak of IMP-6-CPE suggested interspecies gene transmission and helped to clarify hidden epidemiological links between cases.
Colored Polymeric Nanofiber Loaded with Minoxidil Sulphate as Beauty Coverage and Restoring Hair Loss
Authors: Sfouq Aleanizy Fadilah , Yahya Alqahtani Fulwah , Alkahtani Hamad M. , Alquadeib Bushra , Eltayeb Esraa K. , Aldarwesh Amal , Abdelhady Hosam G. , Alsarra Ibrahim A. 
Published: March 2020

AbstractPolymeric nanofibers fabricated by electrospinning either blank (PVA) or loaded with minoxidil sulphate have yielded optimum fibers with an average diameter 273 nm, and 511 nm, respectively. Thermal analysis of nanofibers indicated no chemical interaction. The NMR spectrum confirmed stability of nanofiber as there were no interactions between functional groups. Prepared nanofibers showed a 47.4% encapsulation efficiency and 73% yield. In vitro drug release of minoxidil sulphate from nanofiber exhibited an initial burst release followed by a slower release pattern. Stability studies revealed that minoxidil nanofiber was stable if stored at room temperature and protected from light with only loss of 9.6% of its nominal concentration within 6 months. As a result, the prepared solid/colored formula serves as an ideal formulation for such instable drug in liquid formula taking the advantage of the attractiveness of beauty colored coverage, and the simple, and non-tousled application.
Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India
Authors: Sharma Priyanka , Thakur Debajit 
Published: March 2020

AbstractActinobacteria is a goldmine for the discovery of abundant secondary metabolites with diverse biological activities. This study explores antimicrobial biosynthetic potential and diversity of actinobacteria from Pobitora Wildlife Sanctuary and Kaziranga National Park of Assam, India, lying in the Indo-Burma mega-biodiversity hotspot. A total of 107 actinobacteria were isolated, of which 77 exhibited significant antagonistic activity. 24 isolates tested positive for at least one of the polyketide synthase type I, polyketide synthase type II or non-ribosomal peptide synthase genes within their genome. Their secondary metabolite pathway products were predicted to be involved in the production of ansamycin, benzoisochromanequinone, streptogramin using DoBISCUIT database. Molecular identification indicated that these actinobacteria predominantly belonged to genusStreptomyces, followed byNocardiaandKribbella. 4 strains, viz.Streptomycessp. PB-79 (GenBank accession no. KU901725; 1313 bp),Streptomycessp. Kz-28 (GenBank accession no. KY000534; 1378 bp),Streptomycessp. Kz-32 (GenBank accession no. KY000536; 1377 bp) andStreptomycessp. Kz-67 (GenBank accession no. KY000540; 1383 bp) showed ~89.5% similarity to the nearest type strain in EzTaxon database and may be considered novel.Streptomycessp. Kz-24 (GenBank accession no. KY000533; 1367 bp) showed only 96.2% sequence similarity toS. malaysiensisand exhibited minimum inhibitory concentration of 0.024 µg/mL against methicilin resistantStaphylococcus aureusATCC 43300 andCandida albicansMTCC 227. This study establishes that actinobacteria isolated from the poorly explored Indo-Burma mega-biodiversity hotspot may be an extremely rich reservoir for production of biologically active compounds for human welfare.
Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6
Authors: Rodríguez Miguel , Torres Marta , Blanco Lydia , Béjar Victoria , Sampedro Inmaculada , Llamas Inmaculada 
Published: March 2020

AbstractGiven the major threat of phytopathogenic bacteria to food production and ecosystem stability worldwide, novel alternatives to conventional chemicals-based agricultural practices are needed to combat these bacteria. The objective of this study is to evaluate the ability of Pseudomonas segetis strain P6, which was isolated from the Salicornia europaea rhizosphere, to act as a potential biocontrol agent given its plant growth-promoting (PGP) and quorum quenching (QQ) activities. Seed biopriming and in vivo assays of tomato plants inoculated with strain P6 resulted in an increase in seedling height and weight. We detected QQ activity, involving enzymatic degradation of signal molecules in quorum sensing communication systems, against a broad range of N-acylhomoserine lactones (AHLs). HPLC-MRM data and phylogenetic analysis indicated that the QQ enzyme was an acylase. The QQ activity of strain P6 reduced soft rot symptoms caused by Dickeya solani, Pectobacterium atrosepticum and P. carotovorum on potato and carrot. In vivo assays showed that the PGP and QQ activities of strain P6 protect tomato plants against Pseudomonas syringae pv. tomato, indicating that strain P6 could have biotechnological applications. To our knowledge, this is the first report to show PGP and QQ activities in an indigenous Pseudomonas strain from Salicornia plants.
Tuning up microbiome analysis to monitor WWTPs’ biological reactors functioning
Authors: de Celis Miguel , Belda Ignacio , Ortiz-Álvarez Rüdiger , Arregui Lucía , Marquina Domingo , Serrano Susana , Santos Antonio 
Published: March 2020

AbstractWastewater treatment plants (WWTPs) are necessary to protect ecosystems quality and human health. Their function relies on the degradation of organic matter and nutrients from a water influent, prior to the effluent release into the environment. In this work we studied the bacterial community dynamics of a municipal WWTP with a membrane bioreactor through 16S rRNA gene sequencing. The main phyla identified in the wastewater were Proteobacteria, Bacteroidetes, Chloroflexi, Planctomycetes and Actinobacteria. The WWTP is located in Spain and, like other studied WWTP in temperate climate zones, the temperature played a major role in community assembly. Seasonal community succession is observed along the two years sampling period, in addition to a continual annual drift in the microbial populations. The core community of the WWTP bioreactor was also studied, where a small fraction of sequence variants constituted a large fraction of the total abundance. This core microbiome stability along the sampling period and the likewise dissimilarity patterns along the temperature gradient makes this feature a good candidate for a new process control in WWTPs.
Genotypic characterization of multiple drug resistant Escherichia coli isolates from a pediatric cancer hospital in Egypt
Authors: Hassan Reem , Tantawy Marwa , Gouda Nouran A. , Elzayat Mariam G. , Gabra Sara , Nabih Amena , Diab Aya A. , El-Hadidi Mohamed , Bakry Usama , Shoeb Mohamed R. , Elanany Mervat , Shalaby Lobna , Sayed Ahmed A. 
Published: March 2020

AbstractInfection with multiple drug resistant (MDR) Escherichia coli poses a life threat to immunocompromised pediatric cancer patients. Our aim is to genotypically characterize the plasmids harbored in MDR E. coli isolates recovered from bacteremic patients of Children’s Cancer Hospital in Egypt 57357 (CCHE 57357). In this study, 21 carbapenem-resistant E. coli (CRE) isolates were selected that exhibit Quinolones and Aminoglycosides resistance. Plasmid shot-gun sequencing was performed using Illumina next- generation sequencing platform. Isolates demonstrated resistant to all beta-lactams, carbapenems, aminoglycosides and quinolones. Of the 32 antimicrobial resistant genes identified that exceeded the analysis cutoff coverage, the highest represented genes were aph(6)-Id, sul2, aph(3″)-Ib, aph(3′)-Ia, sul1, dfrA12, TEM-220, NDM-11. Isolates employed a wide array of resistance mechanisms including antibiotic efflux, antibiotic inactivation, antibiotic target replacements and antibiotic target alteration. Sequenced isolates displayed diverse insertion sequences, including IS26, suggesting dynamic reshuffling of the harbored plasmids. Most isolates carried plasmids originating from other bacterial species suggesting a possible horizontal gene transfer. Only two isolates showed virulence factors with iroA gene cluster which was found in only one of them. Outside the realms of nosocomial infections among patients in hospitals, our results indicate a transfer of resistant genes and plasmids across different organisms.
Transcriptomic signatures of brain regional vulnerability to Parkinson’s disease
Authors: Keo Arlin , Mahfouz Ahmed , Ingrassia Angela M. T. , Meneboo Jean-Pascal , Villenet Celine , Mutez Eugénie , Comptdaer Thomas , Lelieveldt Boudewijn P. F. , Figeac Martin , Chartier-Harlin Marie-Christine , van de Berg Wilma D. J. , van Hilten Jacobus J. , Reinders Marcel J. T. 
Published: March 2020

AbstractThe molecular mechanisms underlying caudal-to-rostral progression of Lewy body pathology in Parkinson’s disease remain poorly understood. Here, we identified transcriptomic signatures across brain regions involved in Braak Lewy body stages in non-neurological adults from the Allen Human Brain Atlas. Among the genes that are indicative of regional vulnerability, we found known genetic risk factors for Parkinson’s disease: SCARB2, ELOVL7, SH3GL2, SNCA, BAP1, and ZNF184. Results were confirmed in two datasets of non-neurological subjects, while in two datasets of Parkinson’s disease patients we found altered expression patterns. Co-expression analysis across vulnerable regions identified a module enriched for genes associated with dopamine synthesis and microglia, and another module related to the immune system, blood-oxygen transport, and endothelial cells. Both were highly expressed in regions involved in the preclinical stages of the disease. Finally, alterations in genes underlying these region-specific functions may contribute to the selective regional vulnerability in Parkinson’s disease brains.
Behandlungsfehler bei der Therapie der Rhizarthrose – Eine Analyse der Daten der Norddeutschen Schlichtungsstelle für Arzthaftpflichtfragen
Authors: Güven Asim , Asiri Mohammed Nasser , Allert Sixtus 
Published: March 2020

Zusammenfassung
Hintergrund Die Rhizarthrose ist eine der häufigsten Erkrankungen der Hand, die durch multiple medizinische und paramedizinische Fachdisziplinen behandelt wird. Vor allem die interventionellen und operativen Therapieoptionen bergen aufgrund ihrer Invasivität ein Risiko für Komplikationen und ebendann auch für Behandlungsfehler. Ziel der vorliegenden Arbeit ist die Beurteilung der Qualität der Therapie der Rhizarthrose anhand von Behandlungsfehlervorwürfen.
Patienten und Methoden Von der Norddeutschen Schlichtungsstelle für Arzthaftpflichtfragen wurden die Daten von Behandlungsfehlervorwürfen bei der Hauptdiagnose Rhizarthrose (ICD-10 M18) der Jahre 2010 bis einschließlich 2018 zur Verfügung gestellt. Diese wurden nach behandelnder Fachabteilung aufgeteilt und analysiert. Die nachgewiesenen Behandlungsfehler wurden näher betrachtet und statistisch ausgewertet.
Ergebnisse Insgesamt wurden 44 Behandlungsfehlervorwürfe in den 9 Jahre geäußert. In 16 Fällen wurde ein Behandlungsfehler nachgewiesen (36,4 %): Handchirurgie 8 von 24 (30,0 %), Unfallchirurgie und Orthopädie 6 von 14 (42,9 %), Plastische Chirurgie 1 von 2 (50,0 %), Allgemeinchirurgie 1 von 1 (100,0 %) sowie je 0 von 1 (0,0 %) für die Fachabteilungen Diagnostische Radiologie, Strahlentherapie und Anästhesie. Bei chirurgischen Fachärzten mit der Zusatzqualifikation Handchirurgie kam es deutlich, jedoch nicht signifikant weniger zu Behandlungsfehlern (8 von 24 Fälle, 30 %) als bei chirurgischen Fachärzten ohne Zusatzqualifikation Handchirurgie (8 von 17 Fälle, 47,1 %) haben (p = 0,286).
Schlussfolgerung Bedankt man, dass Operationen der Rhizarthrose zu den häufigsten Eingriffen an der Hand gehören, sprechen die vorliegenden Daten für eine fehlerarme Behandlung der Rhizarthrose in Deutschland. Dabei ist die Rate an Behandlungsfehlern von Handchirurgen deutlich geringer als bei chirurgischen Fachärzten ohne Zusatzbezeichnung Handchirurgie.
Carotid Intima-Media Thickness is a Predictor of Subclinical Myocardial Damage in Men with Type 2 Diabetes Mellitus
Authors: Hörber Sebastian , Lehn-Stefan Angela , Hieronimus Anja , Hudak Sarah , Fritsche Louise , Fritsche Andreas , Heni Martin , Häring Hans-Ulrich , Peter Andreas , Randrianarisoa Elko 
Published: March 2020

Abstract
Background Type 2 diabetes mellitus (T2DM) promotes the development of atherosclerosis and is a major risk factor for cardiovascular disease. High-sensitivity cardiac troponin I (hs-cTnI) assays fundamentally improved the diagnosis of myocardial injury and even enable the prediction of future cardiovascular events in the general population. However, data about the association of hs-cTnI with cardiovascular risk factors and carotid intima media thickness (cIMT) as a marker of atherosclerosis are limited, especially in patients with T2DM.
Methods In this cross-sectional study we analyzed clinical and laboratory parameters of 234 patients (43% women) with T2DM and a median age of 65 years (interquartile range: 57–71). The median duration of diabetes mellitus was 10 years (6–17). Anthropometric data, blood pressure, glycemic parameters and lipid profiles were determined. Hs-cTnI plasma concentrations were measured on an ADVIA Centaur XPT immunoassay analyzer and cIMT was evaluated by high-resolution ultrasound.
Results Hs-cTnI plasma concentrations were below the gender-specific 99th  percentile in 93% of T2DM patients with a median concentration of 4.0 ng/l (interquartile range: 2.0–10.0). Hs-cTnI was significantly associated with gender, renal function and C-reactive protein in the entire study cohort. Gender-specific analyses revealed cIMT and renal function to be significantly associated with hs-cTnI in men. Contrary, only age was significantly associated with hs-cTnI in women.
Conclusion In a real-world clinical setting in patients with T2DM, cIMT is a predictor of subclinical myocardial damage in men, but not in women.
Acute Mesenteric Ischemia Remains a Highly Morbid Diagnosis after Initial Hospitalization Survival
Authors: Erben Y. , Spaulding A. C. , Oderich G. S. , Da Rocha-Franco J. A. , Farres H. , Cochuyt J. J. , Sorrells W. S. , Oldenburg A. W. , Frey G. T. , Toskich B. B. , Becher R. , Hakaim A. G. 
Published: March 2020

AbstractAcute mesenteric ischemia (AMI) remains a vascular emergency. Our aim was to explore readmission for AMI. We identified all patients admitted for AMI from the state of California through the Healthcare and Utilization Project from 2005 to 2011. Our primary end point was the rate and etiology for readmission. Our secondary end points were the length of hospitalization and in-hospital mortality. Cox proportional hazard regression was utilized to assess risk of 30-day readmission. There were 534 (9.9%) readmissions at 30 days. The mean age was 67 ± 17 years and 209 (39.1%) were male. The five most common etiologies for readmission were AMI (7.6%), cardiac events (5.3%), severe sepsis (1.2%), dehydration (1.1%), and acute kidney failure (1.1%). Once readmitted, these patients were most likely to experience cardiac catheterizations (25.4%), red blood cell transfusions (23.6%), intubation and mechanical ventilation (17.6%), biopsy of the large intestine (13.9%), reoperation for small bowel resection (10.9%), administration of total parenteral nutrition (10.5%), and transfusion of other blood products (6.9%). This hospitalization was 8.8 ± 12.7 days long. In-hospital mortality was 36 patients (6.7%). On multivariable Cox-regression analysis, severe (hazard ratio [HR]: 2.1 [1.4–3.2], p = 0.0005) and moderate (HR: 1.5 [1.03–2.13], p = 0.04) Elixhauser Comorbidity Group, complications (HR: 1.5 [1.2–1.9], p = 0.0007), and longer index hospitalization (HR: 1.02 [1.01–1.02], p < 0.0001) were predictors of readmission. Conclusion AMI remains a vascular emergency. Readmissions have a significant rate of morbid invasive procedures and can lead to an in-hospital mortality of 6.7%. The adoption of guidelines similar to the European Society for Trauma and Emergency Surgery should be considered.
Development and Deployment of an Intraoperative Clinical Summary Document: A Veterans Health Care Affairs Perspective
Authors: Mudumbai Seshadri C. , Tanaka Troy , Mariano Edward R. 
Published: March 2020

Abstract
Background Clinical summary documents that abstract details of episodes of care are essential to Advancing Care Information requirements for electronic health records. Few examples exist for intraoperative clinical summary documents (ICSD).
Methods We describe the development and deployment of an ICSD at a tertiary hospital within the United States. Development included identification of needs of key stakeholders, evaluation of current clinical and data workflows, iterative development of prototypes with primary stakeholders, i.e., anesthesiologists and evaluation of prototypes with test patients. We deployed the ICSD over 6-months with tracking of (1) usage (number and types of end users and surgery types) and (2) written and oral feedback.
Results Current workflows involved accessing a 10 to 40 page document presenting all surgery details with review described as burdensome. The ICSD prototype was a separate one-page summary optimized for viewing on a monitor or paper. The document had four sections: (1) administrative data, allergies, and precautions; (2) medications, infusions, and fluid intake and output; (3) airway assessments and a graphical presentation of hemodynamic trends (blood pressure), and (4) standardized text for events (hypotension) occurring intraoperatively. During the 6-month deployment, postanesthesia care unit and intensive care unit nurses were most prominent users followed by anesthesiologists. While overall well received, our pilot users reported challenges for readability, font size, and the lack of customization.
Conclusion While the ICSD was designed for anesthesiologists, postanesthesia care unit and intensive care unit nurses were the most frequent users. Future development will involve customization for different stakeholders.
Platelet Dysfunction in Noonan and 22q11.2 Deletion Syndromes in Childhood
Authors: Ruiz-Llobet Anna , Isola Ignacio , Gassiot Susanna , Català Albert , Díaz-Ricart Maribel , Martínez-Monseny Antonio F. , Serrano Mercedes , Berrueco Rubén 
Published: March 2020

Abstract
Introduction An underlying thrombocytopathy seems to be responsible for hemorrhagic symptoms in patients diagnosed with 22q11.2 deletion syndrome (22q11DS) or Noonan syndrome (NS). In 22q11DS, it is explained by a defect in the membrane glycoprotein (GP) complex Ib-V-IX. The cause of thrombocytopathy in NS remains unclear.
Aim The objective is to study the incidence of thrombocytopathy in pediatric patients diagnosed with 22q11DS or NS assessing the utility of ISTH-BAT questionnaire and laboratory techniques.
Materials and Methods Prospective study between March and December 2018 in children (2–18 years old) diagnosed with 22q11DS or NS. Hemorrhagic symptoms using ISTH-BAT score, total cell blood count, platelet indices, PFA-200 closure times, and platelet aggregation were evaluated in all patients and membrane GP expression in 22q11DS patients.
Results Nearly 70% of NS patients (n = 22) had a platelet aggregation defect without thrombocytopenia. A defect of platelet aggregation with adenosine diphosphate (ADP) and epinephrine was the most frequent pattern. A statistically significant inverse correlation between closure times and aggregation with arachidonic acid (p = 0.049, p = 0.043) and epinephrine (p = 0.021, p = 0.035), and ADP (p = 0.117, p = 0.05) was found. Total 5 out of 29 patients diagnosed with 22q11DS had macrothrombocytopenia; more noteworthy in older patients. Twenty-six patients showed an impairment in ristocetin-induced platelet aggregation that correlated with prolonged collagen/epinephrine (p = 0.034) and collagen/ADP (p = 0.01). A significant association between ISTH-BAT score >3 and closure times (p = 0.022, p = 0.002) and aggregation defect with ristocetin (p = 0.043) was also demonstrated.
Conclusion Most NS and 22q11DS patients show an impairment of platelet aggregation that correlates with closure times. In 22q11DS patients, these results were also related to hemorrhagic symptoms.