bluuebunny/crossref_metadata_2025_split huggingface dataset:
142 Mineral requirements of fed cattle
Authors: Hansen Stephanie L 
Published: November 2020

Abstract
The purpose of this presentation is to summarize state of the art data related to our understanding of feedlot cattle mineral requirements. To date, mineral requirements of cattle have been estimated based on historical research, some conducted as early as the 1930s and 40s. In comparison to earlier editions of the beef cattle nutritional requirements, relatively few alterations in mineral feeding recommendations were noted in NASEM (2016). However, consultants working in the industry commonly report feeding minerals far in excess of the NASEM (2016) recommendations. In addition, an abundance of mineral supplementation strategies has contributed to misunderstandings associated with cattle mineral requirements. Trace minerals, in particular, are available in a variety of chemical forms that affect site of solubility, potential bioavailability and may allow supplementation via various routes (dietary, injection, bolus, oral drench). Published and unpublished data comparing national vs. industry recommendations of trace minerals on beef cattle performance and data pertaining to source comparisons in fed cattle will be presented.
293 Evaluation of the Water Footprint of Beef Cattle Production in Nebraska
Authors: Spore Tyler , Mekonnen Mesfin , Neale Christopher , Watson Andrea K , MacDonald James C , Erickson Galen E 
Published: November 2020

Abstract
Data were compiled from an experiment evaluating the effects of month of calving, wintering system, and calf management on cow and calf performance to model the amount of water (green and blue) required to produce beef in Nebraska production systems. The referenced study was conducted over four years utilizing 217 cow/calf pairs per year. Cows were wintered on either native range or corn residue and month of calving was March, June, or August. Calves were managed as calf-feds or yearlings and only steer calves were included in the model. A 365-d period was utilized to estimate the cow’s contribution of water inputs based on dry matter intake throughout the year varying by production system. Diet characteristics, dry-matter intake, days on feed, average daily gain, and hot carcass weight were measurements used to estimate water utilization by the calves. The total water footprint was calculated by dividing the total amount of water used for each system (L) by the amount of boneless beef produced (kg). Production systems where cows were wintered on corn residue utilized 18% less water than systems utilizing native range as a wintering source, because of water allocations using the value fraction method. Increasing the dietary inclusion of distillers grains from 0% to 40% decreased the water footprint in the finishing phase by 29%. Utilizing by-products in replacement of primary crops decreases the overall water footprint of production. Across all scenarios modeled, more than 50% of the water footprint was allocated to the cow grazing rainfed pasture (green water) and over 98% of the water footprint was allocated to growing feed. Improvements in crop water use efficiency will benefit livestock production. The water footprint of the beef systems analyzed was 80% green water as rain, minimizing the environmental impact of beef production on freshwater use and ecological water balance.
295 Impact of increasing levels of fumonisin on performance of finishing beef steers
Authors: Jennings Jenny , Ensley Steve , Lawrence Ty , Lockard Cathy 
Published: November 2020

Abstract
To address the gaps in current scientific knowledge, the objective of the present study was to investigate the impact of fumonisin exposure on feedlot cattle intake and performance. Fifty steers were received (d 0; 361 ± 6.4 kg), and housed individually in 1.8 x 11.0 m pens and fed once daily at 0800 h. Steers were transitioned to a dry-rolled corn-based finishing diet from d 0 to 21 and then were fed the control finishing diet until d 50. Treatment diets were formulated to achieve ≤ 5 ppm (CON), 15 (15PPM), 30 (30PPM), 60 (60PPM), and 90 ppm (90PPM) of total dietary fumonisin. Steers were fed the fumonisin treatment diets from d 50 until harvest on d 160; individual animal body weights were taken on days 0, 50, 100, 150 and 160. Animal performance and carcass data were analyzed using PROC MIXED in SAS with animal as the experimental unit and PROC GLIMMIX was used to analyze categorical carcass data. Contrasts were used to test for linear and quadratic responses. Throughout the study, there was no effect of treatment (P &gt; 0.60), or a linear response (P &gt; 0.16) from increasing fumonisin levels, on BW or DMI. However, CON tended to have lower ADG than the other treatments during the fumonisin treatment period (P = 0.10), and there was a positive linear response (P = 0.02) of ADG to fumonisin dose during the treatment period. Similarly, ADG tended to increase linearly over the entire feeding period (P = 0.06), as did G:F (P = 0.07). There were no differences between treatments regarding HCW, DP, marbling score, ribeye area, or YG. There were no effects of treatment on either liver abscesses (P = 0.95) or telangiectasis (P = 0.13). No differences in body weight, intake, carcass, or liver characteristics were observed in steers fed dietary levels of fumonisin ranging from 8 to 108 ppm for 110 days prior to harvest.
56 Impact of Electronically-Controlled Cooling Pads on Physiological Signs of Heat Stress in Early Lactation Sows
Authors: Whitmore Flannery J , Schinckel Allan P , Stwalley Robert , Field Tyler , Burgett Morgan , Garcia Ricardo M , Johnson Jay S 
Published: November 2020

Abstract
The study objective was to evaluate the impact of electronically controlled cooling pads on physiological measurements of heat stress during early lactation. Twelve sows (d 3 to 11 of lactation) were provided with a specially designed cooling pad. Sows randomly received no cooling (CONTROL, n=6) or pads set to flush 2L cool water every 30s when aluminum plate reached 29.5°C (TREAT, n=6). Respiration rates (RR), rectal temperatures (RT) and skin temperatures (ST, 15 cm posterior to the ear) were recorded daily (0700 and 1500 h) for seven days of early lactation. Feed intake (DFI) was measured daily at 0700 h. RR, RT, and ST were fitted to a model that included the effects of day, time, parity, cooling pad treatment, and interactions. DFI was fitted to the same model, excluding time of day. For RR: treatment, day, time, treatment by day, treatment by time, and day by time interactions were significant (P&lt; 0.0004, CONTROL=49, TREAT= 30). Pad treatment had greater impact on RR during the first two days of the trial when the temperature was greater (32°C maximum CONTROL mean=66 vs. TREAT mean=30) than the following five days: 42, and 30 respectively). There was greater treatment impact on RR at 1500 versus 0700 h (0700 h: CONTROL= 35, TREAT= 25; 1500 h: CONTROL= 61, TREAT= 36). For RT: treatment, day, time, parity and interaction of day by time were significant (P&lt; 0.01, CONTROL= 37, treat= 35.). For ST: treatment, day, time, parity and the interactions of time by parity, treatment by time, and day by time were significant (P&lt; 0.0006, mean CONTROL=36.5, TREAT= 35.1). For DFI: day and the interaction of day by treatment were significant. The difference in feed intake between treatments increased as day increased. The use of electronically controlled cooling pads reduced measures of heat stress in early lactating sows.
PSV-15 Genomic basis of reproductive performance in PRRSV-infected sows
Authors: Hickmann Felipe , Neto José Braccini , Kramer Luke , Gray Kent , Huang Yijian , Dekkers Jack , Sanglard Letícia P , Serão Nick 
Published: November 2020

Abstract
Previous studies have shown that reproductive traits in sows during porcine reproductive and respiratory syndrome (PRRS) outbreaks are lowly heritable. However, little is known about the genomic basis of reproductive performance in PRRS virus (PRRSV)-infected sows. Thus, the objective of this work was to evaluate the genomic basis of reproductive performance in PRRSV-infected sows. Farrowing data from 475 Duroc and 405 Landrace sows that experienced a PRRS outbreak were used. Heritability and genome-wide association studies (GWAS) based on ~30K SNP genotypes were performed for each breed separately, for number of piglets born alive (NBA), stillborn piglets (NSB), mummified piglets (NM), piglets born dead (NBD; NSB+NM), total number of piglets born (TNB; NBA+NBD), and piglets weaned (NW). Heritabilities were estimated with ASReml using a model with the fixed-effect of parity and an animal random-effect, using a genomic-relationship matrix. GWAS was performed using Bayes-B in GenSel with a model fitting parity as fixed-effect and SNP effects as random. Heritability estimates (± standard error) for NBA, NSB, NM, NBD, TNB, and NW were 0.07±0.07, 0.02±0.11, 0.04±0.10, 0.08±0.08, 0.08±0.07, 0.06±0.04, respectively, for Duroc, and 0.09±0.07, 0.10±0.07, 0.10±0.08, 0.11±0.07, 0.07±0.08, 0.07±0.08, respectively, for Landrace. For Duroc, GWAS identified a quantitative trait locus (QTL) on chromosome (chr) 3 [114-120 megabases (Mb)] explaining 3.9% of the genetic variance (GV) for NBA, on chr 13 (189-193 Mb) explaining 2.7% GV for NBD, and on chr 5 (36-41 Mb) explaining 4.3% GV for TNB. For Landrace, GWAS identified a QTL on chr 13 (154-162 Mb) explaining 12.8% GV for NBA, on chr 14 (59-69 Mb) explaining 2.5% GV for NBD, and on chr 10 (7-9 Mb) explaining 4.1% GV for NW. These results indicate that reproductive traits are lowly heritable during a PRRS outbreak with few QTL identified in Duroc and Landrace sows.
PSV-11 Accuracies of genomic prediction for reproductive traits in PRRSV-infected sows
Authors: Hickmann Felipe , Neto José Braccini , Kramer Luke , Gray Kent , Huang Yijian , Dekkers Jack , Sanglard Letícia P , Serão Nick 
Published: November 2020

Abstract
Recent studies on host-genetics of response to porcine reproductive and respiratory syndrome virus (PRRSV) infection have shown that genetic markers can be used to predict performance in growing pigs. On the other hand, no work has been done on the genomic prediction of reproductive performance in PRRSV-infected sows. Thus, the objective of this work was to assess the accuracies of genomic prediction for reproductive traits in PRRSV-infected sows. A total of 475 Duroc and 405 Landrace sows with ~30K SNP genotypes had farrowing performance data during the outbreak on: number of piglets born alive (NBA), stillborn piglets (NSB), mummified piglets (NM), piglets born dead (NBD; NSB+NM), total number of piglets born (TNB; NBA+NBD), and piglets weaned (NW). Genomic prediction was performed by Bayes-B with a model fitting parity as fixed-effect and SNP effects as random. Analyses were performed for two scenarios: within-breed and between-breed. For the within-breed prediction, 4-fold cross-validation was used. For the between-breed prediction, all data for each breed were used. Accuracy of genomic prediction (AGP) was calculated as the correlation between genomic estimated breeding values and pre-adjusted phenotype, divided by square-root of heritability. For the within-breed prediction, AGP (± standard deviation) for NBA, NBD, NM, NSB, NW, and TNB were 0.24±0.05, 0.07±0.10, -0.27±0.06, -0.24±0.09, -0.05±0.05, 0.19±0.03, respectively, for Duroc, and 0.37±0.02, 0.38±0.11, 0.30±0.07, 0.60±0.10, 0.09±0.11, 0.15±0.10, respectively, for Landrace. For the between-breed prediction, AGP for NBA, NBD, NM, NSB, NW, and TB were 0.28, -0.17, -0.01, 0.37, 0.27, and 0.29, respectively, when training on Landrace and validating on Duroc, and 0.28, -0.14, 0.01, 0.15, 0.23, and -0.01, respectively, when training on Duroc and validating on Landrace. Results show that the accuracies of genomic prediction for reproductive traits during a PRRSV outbreak are low to moderate.
PSV-16 Influence of the late gestation maternal rumen microbiome on the calf meconium and early rumen microbiome
Authors: Woodruff Kelly , Hummel Gwendolynn , Austin Kathleen , Smith Travis , Cunningham-Hollinger Hannah 
Published: November 2020

Abstract
Understanding the development of the calf rumen microbiome is important in developing manipulation strategies to improve efficiency as the animal ages. We hypothesized that the cow maternal microbiome would influence the colonization of the calf rumen microbiome. Our objective was to relate the microbiomes of the cow rumen fluid (RFC) to the calf meconium (M) and calf rumen fluid (RFN) at twenty-eight days of age. Mature, multiparous Angus crossbred cows (n = 10) from the University of Wyoming beef herd were used in this study. Rumen fluid was collected from the cows prior to parturition. Immediately following parturition, meconium was collected from the calf and at 28 days post-parturition, rumen fluid was collected from the calves. Microbial DNA was isolated using a lysis buffer and mechanical bead-beating procedure and purified using the QIAamp DNA Stool Mini Kit (Qiagen). Amplicon sequencing of the 16S rRNA V4 region was completed on the MiSeq and analyzed with QIIME2. Both alpha and beta diversity were evaluated by sample type and day. Richness and evenness differed by sample type. The greatest richness and evenness was in RFC (q &lt; 0.01) followed by RFN and M, which did not differ from each other (q ³ 0.5). Bray-Curtis and Jaccard beta diversity differed by each sample type (q &lt; 0.01). These data indicate that the M and RFN do not differ in number and distribution of features, but the samples are compositionally different. Additionally, the RFC differed in both alpha and beta diversity from both calf samples. These profiles can be used to develop hypotheses for the pathway of colonization in the early gut yet still reflect the vast differences in the developmental stage between the cow rumen microbiome and the early calf gastrointestinal microbiome.
PSVII-2 Differentially expressed genes and their biological function in skeletal muscle of calves born from cows with or without protein supplementation during mid-gestation
Authors: Carvalho Elisa B , Sanglard Letícia P , Nascimento Karolina B , Meneses Javier M , Casagrande Daniel R , Duarte Marcio , Gionbelli Mateus P , Serão Nick 
Published: November 2020

Abstract
Gestating cows have an increased nutrient demand to meet the needs of developing the fetus and the mid-gestation is a critical period for the fetal skeletal muscle development. The aim of this study was to evaluate the skeletal muscle transcriptome in the progeny as a function of the maternal protein nutrition during mid-gestation. Eleven Tabapuã cows and their male calves were used in this study. In the first third of gestation (0 to 100 days of gestation; dg), all cows were kept on pasture. From 100 to 200 dg, the control group (CTRL; 7 animals) received a basal diet achieving 5.5% crude protein (CP), whereas the supplemented group (SUPPL; 4 animals) received a basal diet plus protein supplementation (40% CP). After 200 dg, all animals received the same diet. Weaning was performed at 205 ± 7.5 days of age and animals were kept on pasture until reaching 240 days of age, when they were transferred to a feedlot. Muscle samples were collected at 260 days of age and RNA was extracted for RNA-seq analysis. Gene expression data was analyzed with a negative binomial model to identify (q-value ≤ 0.05) differentially expressed genes (DEG) between treatments. A total of 716 DEG were identified (289 DEG up-regulated and 427 down-regulated in SUPPL group; q-value ≤ 0.05). From the 10 most significant down-regulated DEG in the SUPPL group, two genes associated with apoptotic process were identified: MAPK8IP1 and GRINA, with log2 Fold-Changes (log2FC) of 1.04 and 0.49, respectively. From the 10 most significant up-regulated DEG in the SUPPL group, mTOR was identified, with log2FC=0.31. This is a well-known gene involved in muscle protein synthesis. In conclusion, maternal protein supplementation during mid-gestation affects the expression of genes related to energy metabolism and muscle development, which can lead to long-term impacts on production efficiency.
PSVII-10 Skeletal muscle transcriptome reveals gene expression differences in newborn goats' as a result of maternal feed restriction at different stages of gestation
Authors: Costa Thaís , Mendes Tiago , Moura Felipe , Souza Ranyeri , Fontes Marta , Lopes Mariana , Serão Nick , Silva Fabyano , Gionbelli Mateus P , Duarte Marcio 
Published: November 2020

Abstract
The current study aimed to identify differentially expressed genes and their biological process in the skeletal muscle of newborn goats as a function of maternal feed restriction during different stages of gestation. A total of 14 pregnant dams were randomly divided into one of the follow dietary treatments: Animals fed at 50% of maintenance requirement from 8-84 d of gestation and then fed at 100% of maintenance requirement from day 85 of gestation to parturition (RM, n = 6), and animals fed at 100% of maintenance requirement from 8-84 d of gestation and then fed at 50% of maintenance requirement from day 85 of gestation to parturition (MR, n = 8). At birth, all male offspring were euthanized and a sample of Logissimus muscle was collected for total RNA extraction and sequencing. Cuffdiff tool (Cufflinks 2.2.1) was used to count reads, normalize transcript expression (FPKM), and identify differentially expressed genes (DEG) between treatments. Network analysis was performed with String 11.0 using the available genome from the closest specie (Ovis aries). A total of 66 DEG (q-value &lt; 0.05) were identified, with 6 up-regulated genes in skeletal muscle of RM compared to MR newborn goats. These genes are related to tissue development, such as CYTL1, UGT8, and NPNT. The 60 down-regulated genes in skeletal muscle of RM compared with MR newborn goats are related to transcription factors (TF) complex. Among the TF, FOS and JUNB families forms heterodimers in response to several stimuli (cytokines,growth factors, stress), and play key roles controlling a number of cellular processes, including signal transduction, cell proliferation, differentiation and apoptosis. In conclusion, maternal feed restriction at different stages of gestation affects tissue development and changes the transcription pattern in the skeletal muscle of newborn goats, which may lead to negative consequences in animal growth and performance.
PSIV-22 Supplemental effects of dietary lysophospholipids in lactation diets on sow performance, milk composition, and intestinal health of piglets
Authors: Jang Kibeom , Purvis Jerry , Kim Sung Woo 
Published: November 2020

Abstract
Dietary lysophospholipids could enhance nutrient utilization through a structural change of enterocyte membrane with increasing permeability. The objective of this study was to determine supplemental effects of dietary lysophospholipids in lactation diets on sow performance, milk characteristics, and intestinal health of piglets. The 52 pregnant sows were allotted to 2 treatments in randomized complete block design with parity and BW as blocks at d 110 of pregnancy. The treatments were CON (no added lysophospholipids) and LPL (at 0.05% lysophospholipids; Lipidol-Ultra, Pathway Intermediates, Shrewsbury, UK). The lactation diets were formulated to meet or exceed nutrient requirements suggested by NRC (2012). Milk samples from 12 sows per treatment were collected to measure gross energy, protein, fat, fatty acid profile, and immunoglobulins (IgG and IgA) on d 1 and d 18 of lactation. Twelve piglets per treatment were euthanized on d 18 to collect tissues to measure tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), malondialdehyde, protein carbonyl, IgA, microbiota in jejunal and colonic mucosa, morphology and crypt cell proliferation rate in the jejunum. Data were analyzed using the MIXED procedure of SAS. Sows fed LPL tended to increase (P = 0.084) litter size (11.9 vs. 12.6) on d 18 of lactation and decrease (P = 0.079) ADFI (8.72 vs. 8.02 kg) during d 9 to d 18 of lactation. Sows fed LPL tended to increase (P = 0.092) IgG (1.14 vs. 1.94 g/L) in the milk. Sows fed LPL increased (P &lt; 0.05) crypt cell proliferation rate (39.38 vs. 40.94%) in the jejunum. Supplementation of lysophospholipids in lactation diet did not affect proinflammatory cytokines, oxidative stress markers, and microbiota in jejunum and colon of piglets on d 18 of lactation. In conclusion, supplementation of dietary lysophopholipids improved productive performance and the intestinal cell proliferation of piglets with enhancing IgG concentration in the milk.
PSIV-19 Evaluation of Enogen® high amylase corn on growth performance and carcass characteristics of finishing pigs
Authors: Ochonski Patricia , Wu Fangzhou , Tokach Mike D , DeRouchey Joel M , Dritz Steve S , Goodband Robert D , Woodworth Jason C , Lattimer James M 
Published: November 2020

Abstract
A total of 288 pigs (Line 600 241, DNA, Columbus, NE; initially 41.6 kg) were used in an 82-d trial to determine if replacing conventional yellow dent corn with high amylase corn (Enogen®, Syngenta Seeds, LLC, Downers Grove, IL) in diets with or without distillers dried grains with solubles (DDGS) influences growth performance and carcass characteristics. Diets were arranged in a 2 × 2 factorial with 2 corn sources (yellow dent or high amylase) and 2 levels of DDGS (0 or 25%). Experimental diets were fed in meal form in 3 phases: d 0 to 29, 29 to 47, and 47 to 82. There were 9 pens/treatment each containing an equal number of barrows and gilts. Pigs were weighed approximately every 2 wk and at the beginning of each phase. On d 82, pigs were transported to a commercial packing plant for processing and carcass data collection. Data were analyzed using PROC GLIMMIX procedure of SAS with pen as the experimental unit. There were no interactions (P &gt; 0.05) between corn source and DDGS inclusion for overall performance or carcass characteristics. Overall, average daily gain (ADG) was marginally greater (P &lt; 0.089) for pigs fed high amylase than yellow dent corn with no evidence (P &gt; 0.196) for difference in feed intake, feed efficiency (G/F), hot carcass weight (HCW), or other carcass traits. Addition of DDGS resulted in decreased (P &lt; 0.037) overall ADG and G/F. Pigs fed DDGS had marginally lower (P &lt; 0.071) HCW, less (P &lt; 0.050) backfat, greater (P &lt; 0.026) loin depth, and greater (P &lt; 0.020) percentage lean and iodine values. In summary, high amylase corn tended to improve overall ADG; however, feed efficiency and carcass characteristics were unchanged between corn sources. Consistent with previous research, addition of DDGS decreased ADG and increased IV.