bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of nicotine content in tobacco through microbial degradation: research progress and potential applications
Authors: Li Zi-Jia , Yang Dong-Dong , Wei Zhi-Yun , Huang Jie , Chi Yi-Qian , Lu You-Xuan , Yin Feng-Wei 
Published: December 2024

AbstractOriginally native to South America, tobacco and is now distributed worldwide as a major cash crop. Nicotine is the main harmful component of tobacco leaves, cigarette smoke and tobacco waste, which severely affects not only the flavor of the tobacco leaf, but also causes great damage to human health. As the anti-smoking movement continued to grow since the 1950s, and consumers become more aware of their health and environmental protection, the world tobacco industry has been committed to research, develop and produce low nicotine cigarette products with relatively low risk to human health. Among various approaches, the use of microorganisms to reduce nicotine content and improve tobacco quality has become one of the most promising methods. Due to increasing interest in nicotine-degrading microorganisms (NDMs), this article reviews recent reports on NDMs, nicotine-degrading enzymes, regulation of nicotine-degrading bacterial consortia and optimization of fermentation conditions, aiming to provide updated references for the in-depth research and application of microorganisms for the degradation of nicotine.
Graphical Abstract
FcRγIIA attenuates pathology of cutaneous leishmaniasis and modulates ITAMa/i balance
Authors: Hammi Ikram , Giron-Michel Julien , Riyad Myriam , Akarid Khadija , Arnoult Damien 
Published: December 2024

Abstract
Background
Leishmania is the causal parasite of leishmaniasis, a neglected tropical disease affecting millions of individuals worldwide, and its dissemination is linked to climate change. Despite the complexity and effectiveness of the immune response, the parasite has developed many strategies to evade it and take control of the host cell to replicate. These evasion strategies start at early stages of infection by hijacking immune receptors to mitigate the cellular response. In this study, we examined whether Leishmania uses the Fc receptor FcγRIIA/CD32a and its downstream signaling pathways to evade the host immune response.

Methods
Regarding in vivo studies, CD32a transgenic mice and the corresponding wild types were infected with Leishmania major Friedlin strain. For the in vitro experiments, BMDMs isolated from WT or CD32a transgenic mice and control or CD32a knockdown differentiated THP-1s were infected with two species of Leishmania, Leishmania major and L. tropica.

Results
In vivo, expression of FcγRIIA/CD32a was found to accelerate the signs of inflammation while simultaneously preventing the formation of necrotic lesions after Leishmania infection. In infected macrophages, the presence of FcγRIIA/CD32a did not affect the secretion of proinflammatory cytokines, while the balance between ITAMa and ITAMi proteins was disturbed with improved Fyn and Lyn activation. Unexpectedly, infection with L. tropica but not L. major triggered an intracytoplasmic processing of FcγRIIA/CD32a.

Conclusions
Our observations underscore the significance of FcγRIIA/CD32a in cutaneous leishmaniasis and its potential use as a therapeutic target.

Graphical Abstract
Fecal carriage of ESBL-producing E. coli and genetic characterization in rural children and livestock in the Somali region, Ethiopia: a one health approach
Authors: Muhummed Abdifatah , Alemu Ashenafi , Hosch Salome , Osman Yahya , Tschopp Rea , Yersin Simon , Schindler Tobias , Hattendorf Jan , Zinsstag Jakob , Cissé Guéladio , Vonaesch Pascale 
Published: December 2024

Abstract
Background
The emergence and spread of Extended-Spectrum Beta-Lactamase (ESBL)-producing Escherichia coli pose significant challenges for treatment of infections globally. This challenge is exacerbated in sub-Saharan African countries, where the prevalence of ESBL-producing E. coli is high. This, combined with the lack of a strong and supportive healthcare system, leads to increased morbidity and mortality due to treatment failures. Notably, studies in Ethiopia have primarily focused on hospital settings, leaving a gap in understanding ESBL prevalence in rural communities, where human-animal proximity may facilitate microbial exchange.

Methods
We conducted a community-based study in the rural Somali region of Ethiopia, simultaneously examining the fecal carriage of ESBL-producing E. coli in children aged 2–5 years and their livestock (cattle, camel, goat). Fecal samples from 366 children and 243 animals underwent phenotypic screening for ESBL-producing E. coli. Following phenotypic confirmation, ESBL resistance genes were identified via conventional PCR. Whole-genome sequencing (WGS) was performed on a subset of isolates from human feces.

Results
We found that 43% (159/366) of children and 3.7% (9/244) of livestock harbored ESBL-producing E. coli. The ESBL gene blaCTX-M-15 was predominant in human (82.7%, 120/145) and livestock (100%) isolates. In the 48 human E. coli isolates subjected to WGS, a high diversity resulting in 40 sequence types (STs) was observed. Among these, ST-2353 was the most prevalent (5/48), followed by ST-10 and ST-48 (3/48) and ST-38, ST-450, and ST-4750 (2/48). These STs were associated with multiple resistance genes, such as blaCTX-M-15, blaTEM-1B, blaOXA-1, blaCTX-M-14 and blaTEM-35.

Conclusion
We report a high prevalence of ESBL E. coli in rural children, which outnumbers its prevalence in livestock. These isolates displayed a high diversity of sequence types (STs) with ST-2353 being the dominant ST. Our study is the first to report the association of ST-2353 with multi-drug resistance genes in Ethiopia. Further research using an integrated approach including other domains such as water and food products is needed to truly understand and combat AMR transmission and acquisition in this region.
Characteristics, opportunities, and challenges of osteopathy (COCO) in the perceptions of osteopaths in Germany, Austria, and Switzerland: a metasynthesis
Authors: Manschel Jonas , Porthun Jan , Beuckels Jean Marie A. T. , Martin David 
Published: December 2024

Abstract
Background
The practice of osteopathy in Europe is not uniformly regulated. Even despite the topographical and cultural proximity, the regulation of the osteopathic profession also differs in the German-speaking countries. In contrast to Germany and Austria, both without any recognized osteopathic profession, Switzerland has already created legal regulations for the practice of osteopathy. The aim of this analysis and the project itself is to gain knowledge about the characteristics, challenges and opportunities of osteopaths in German-speaking countries.

Methods
The COCO project examines osteopaths in Germany, Austria, and Switzerland, considering their view on the practice of osteopathy. Within the framework of a metasynthesis, a systematic literature search was conducted first to identify further relevant studies. Subsequently, a qualitative synthesis was followed after having applied the inclusion and exclusion criteria to the literature found.

Results
This study was able to draw on a total of 30 content-analyzed interviews with osteopaths. It was possible to gain deeper insights into the characteristics, challenges and opportunities of osteopaths in German-speaking countries.
Challenges were diagnosed, for example, regarding professional identity, as well as in the intraprofessional conflicts and the question of standards in training and research. The chances were described as leading a fulfilling professional life and meeting a great interest in osteopathy among the population.

Conclusions
Regarding the characteristics of osteopathy in Germany, Austria and Switzerland, it can be said that osteopaths have difficulties to define osteopathy. The resources mentioned most often are manual work on the patient, a holistic treatment approach and generous treatment durations. Lecturers and prominent figures can be seen as role models and greatly influence the perspective of osteopaths. The central challenge is the problem of identity among osteopaths. In addition, different training programs and qualifications exist, which also presents the greatest country-specific differences. The satisfaction of osteopaths in their work and the extreme demand for osteopathy in society should be seen as an opportunity for the field.
A mobile-based randomized controlled trial on the feasibility and effectiveness of screening for major depressive disorder: study protocol
Authors: Zandbergen M. M. E. , Jansen E. E. L. , Jabbarian L. J. , de Koning H. J. , de Kok I. M. C. M. 
Published: December 2024

Abstract
Background
Mobile-based screening interventions to detect and treat Major Depressive Disorder (MDD) at an early stage might be a promising approach for reducing its societal burden. In the present study, we will evaluate the feasibility and effectiveness of screening for MDD using a mobile-based screening protocol.

Methods
This study will be a three-arm, parallel randomized control trial (RCT) performed in a multi-ethnic population within the municipality of Rotterdam (the Netherlands). The trial includes two intervention groups that will be screened 4-weekly for MDD for 12 months using the Patient Health Questionnaire (PHQ-9) and a control group who does not receive mobile-based screening for MDD. Participants in the one-test intervention arm will be referred for further diagnosis and treatment, if necessary, after a single positive test score for moderate-severe major depression symptoms (PHQ-9 > 10). Participants in the multiple-test intervention arm will only be referred after three consecutive positive test scores. 1786 eligible participants will be included in the RCT, with 446 and 447 in the one-test and multiple-test referral arms, respectively, and 893 in the control arm. Primary outcome is participants’ QoL after 12 months (EQ-5D-5L). Secondary outcomes include participants’ QoL after 24 months (EQ-5D-5L), evaluating the occurrence and severity of MDD symptoms (PHQ-9), intervention engagement, and identifying public mental health differences based on sociodemographic characteristics, including age, gender, ethnicity, financial situation, educational background, and living area. Long-term results of the RCT will be incorporated into a microsimulation model to determine the long-term benefits, harms, and costs of MDD screening.

Discussion
The information gained from examining the feasibility and (cost-) effectiveness of mobile-based screening for MDD could be of guidance for mental health policy implementations and support the introduction of mobile-based screening for MDD in the Netherlands and/or other nations.

Trial registration
ClinicalTrials.gov: NL84280.078.23, NCT05989412, August 8, 2024.
Mothers balancing work and family: the associations with emotional well-being, sleep–wake problems and the role of basic needs
Authors: Olivieri Rosalia , Lo Presti Alessandro , Costa Sebastiano , Ariemma Lucia , Fabbri Marco 
Published: December 2024

Abstract
Background
The transition to motherhood involves significant changes, extending from pregnancy to the early years of a child's life. This period, characterized by multiple stressors and adjustments, can profoundly impact maternal well-being. For this reason, the aim of this study was to investigate the associations between work-family balance, need satisfaction, depression, anger, vitality and sleep–wake problems, using Self-determination Theory (SDT) as a theoretical framework.

Methods
Participated in this study 218 working mothers in Italy with children aged between 1 and 36 months. Using a correlational design, SEM with latent variables was used to test an integrative model.

Results
Results revealed significant positive associations between work-family balance and need satisfaction, and vitality, and negative direct associations of need satisfaction with sleep–wake problems, depression, and anger. Indirect associations were also visible between work-family balance and psychological outcomes through need satisfaction.
Conclusions.
Overall, findings underscore the importance of conditions that support the satisfaction autonomy, competence, and relatedness of working mothers during this critical life stage. These insights have implications for organizational policies and interventions aimed at supporting the mental health of working mothers.
mRNA cancer vaccines from bench to bedside: a new era in cancer immunotherapy
Authors: Shariati Alireza , Khani Pouria , Nasri Farzad , Afkhami Hamed , Khezrpour Arya , Kamrani Sina , Shariati Fatemeh , Alavimanesh Sajad , Modarressi Mohammad Hossein 
Published: December 2024

AbstractHarnessing the power of the immune system to target cancer cells is one of the most appealing approaches for cancer therapy. Among these immunotherapies, messenger ribonucleic acid (mRNA) cancer vaccines are worthy of consideration, as they have demonstrated promising results in clinical trials. These vaccines have proven to be safe and well-tolerated. They can be easily mass-produced in a relatively short time and induce a systemic immune response effective against both the primary tumor and metastases. Transcripts encoding immunomodulatory molecules can also be incorporated into the mRNA, enhancing its efficacy. On the other hand, there are some challenges associated with their application, including mRNA instability, insufficient uptake by immune cells, and intrinsic immunogenicity, which can block mRNA translation. Many innovations have been suggested to overcome these obstacles, including structural modification (such as 5’ cap modification), optimizing delivery vehicles (especially dendritic cells (DCs) and nanoparticles), and using antigens that can enhance immunogenicity by circumventing tolerance mechanisms. A popular approach is to combine mRNA cancer vaccines with traditional and novel cancer treatments like chemotherapy, radiotherapy, and immune checkpoint blockade (ICB). They are most efficacious when combined with other therapies like ICBs. There is still a long way to go before these vaccines enter the standard of care for cancer patients, but with the incredible pace of development in this field, their clinical application will soon be witnessed. This review highlights the recent advances and challenges of mRNA cancer vaccines. Finally, some of the most prominent clinical applications of these vaccines will be reviewed.
1,2-DCA biodegradation potential of an aquifer assessed in situ and in aerobic and anaerobic microcosms
Authors: Cruciata Ilenia , Scirè Calabrisotto Laura , Carpani Giovanna , Poppa Lucia , Modica Alfonso , Pace Andrea , Catania Valentina , Quatrini Paola 
Published: December 2024

Abstract
Background
1,2-dichloroethane (1,2-DCA) biodegradation can occur through aerobic or anaerobic pathways that can be exploited in bioremediation strategies. Bioremediation interventions are site specific and generally based on anaerobic pathways, nevertheless expanding knowledge on proper conditions favoring the biodegradation and especially on 1,2-DCA degrading microorganisms is crucial. In this work the intrinsic biodegradation potential of an aquifer impacted by Chlorinated Aliphatic Hydrocarbons (mainly 1,2-DCA) was evaluated by characterizing the aquifer microbiome across space and time and by setting up biostimulation treatments in microcosms under different aerobic and anaerobic conditions, in parallel.

Results
The microbial profiling of the aquifer revealed noticeable alpha and beta diversity across the sampling sites within the aquifer and strong fluctuations over time. Surprisingly both the anaerobic and aerobic biostimulation treatments led to the successful removal of 1,2-DCA in microcosms, the enrichment of known 1,2-DCA degraders and the detection of reductive or hydrolytic dehalogenases. Ancylobacter and Starkeya were enriched in aerobic microcosms. Desulfovibrio and Desulfuromonas, known as perchloroethylene degraders, were enriched in anaerobic microcosms, suggesting they could be yet unknown 1,2-DCA respirers.

Conclusions
Our results demonstrate the occurrence of both aerobic and anaerobic bioremediation potential in the aquifer despite its negative redox potential. Due to the feasibility of direct oxidation with oxygen insufflation, we propose that an enhanced bioremediation strategy based on direct oxidation of 1,2-DCA could be applied to the contaminated aquifer as an ecofriendly, efficient and cost-effective approach as an alternative to anaerobic biodegradation.
Impact of model physiotherapy centres in reducing the burden among the caregivers of children with neurodevelopmental disorders in the rural area of Tamil Nadu: a study protocol
Authors: Kanniappan Vadivelan , Muthuperumal Prakash , Venkataraman P. , Veeragoudhaman T. S. , Chinnasami Balaji , Muthiah Manikumar , Jayapal Rajeswari Abishek , Manoharan Manju Bashini , Suresh Shrisruthi , Natesan Ashok 
Published: December 2024

Abstract
Background
Approximately 1 in 8 children under the age of 6 in Tamil Nadu are diagnosed with neurodevelopmental disorders (NDD), yet only a fraction of these children receives proper medical care. The unequal distribution of healthcare services is mainly due to the lack of accessibility, especially in rural areas, leading to a significant burden on caregivers. This research aims to alleviate caregiver burden and reduce disability in preschool children by establishing model clinics or specialized outreach centers in rural villages, supported by SRM Institute of Science and Technology under the Unnat Bharat Abhiyan Scheme.(UBA-SRMIST).

Methods
The study will commence with screening all preschool children registered in Anganwadi in the designated villages. Tailored model clinics will be set up in these villages based on the prevalence of NDD. Once established, children diagnosed with NDD will undergo rehabilitation for a year. Baseline and endline assessments will be conducted to evaluate the effectiveness of the model center on both the child's disability and the caregiver burden.

Discussion
This research will demonstrate the effectiveness of a model outreach center in rural villages in reducing disability levels in children and alleviating caregiver burden by eliminating the need for long travels to access rehabilitation services. The findings of this study will not only contribute to the objectives of UBA-SRMIST in uplifting villages but also facilitate the creation of registries and provision of data to the government for the implementation of policies that address the current disparities in healthcare access.
Trial registration Trail has been registered under Clinical Trials Registry—India (CTRI/2024/06/069196).
When common cognitive biases impact debriefing conversations
Authors: Meguerdichian Michael J. , Trottier Dana George , Campbell-Taylor Kimberly , Bentley Suzanne , Bryant Kellie , Kolbe Michaela , Grant Vincent , Cheng Adam 
Published: December 2024

AbstractHealthcare debriefing is a cognitively demanding conversation after a simulation or clinical experience that promotes reflection, underpinned by psychological safety and attention to learner needs. The process of debriefing requires mental processing that engages both “fast” or unconscious thinking and “slow” intentional thinking to be able to navigate the conversation. “Fast” thinking has the potential to surface cognitive biases that impact reflection and may negatively influence debriefer behaviors, debriefing strategies, and debriefing foundations. As a result, negative cognitive biases risk undermining learning outcomes from debriefing conversations. As the use of healthcare simulation is expanding, the need for faculty development specific to the roles bias plays is imperative. In this article, we hope to build awareness about common cognitive biases that may present in debriefing conversations so debriefers have the chance to begin the hard work of identifying and attending to their potential detrimental impacts.
GLP-1R Gene Polymorphisms and Metabolic Traits During Childhood and Adolescence: The EPOCH Study
Authors: Harrall Kylie K , Glueck Deborah H , Lange Leslie A , Litkowski Elizabeth M , Vanderlinden Lauren A , Konigsberg Iain R , Cree Melanie G , Perng Wei , Dabelea Dana 
Published: December 2024

Abstract

Context
This is the first study to examine the association between variants of the glucagon-like-peptide-1 receptor gene (GLP-1R) and metabolic characteristics among youth.

Objective
We explored separate associations of 3 GLP-1R polymorphisms (rs10305420, rs6923761, and rs1042044) with body mass index (BMI) trajectories and markers of glucose-insulin homeostasis.

Methods
Mixed models examined associations between GLP-1R polymorphisms and trajectories of BMI. Linear models examined associations of GLP-1R polymorphisms with glucose and insulin concentrations across oral glucose tolerance test (OGTT), insulin sensitivity (HOMA2-IR), insulin secretion (insulinogenic index and HOMA2-%B), and β-cell function (oral disposition index).

Results
Rs10305420 and rs6923761, but not rs1042044, were associated with growth and metabolic characteristics in early life. Rs6923761 genotype GG was associated with faster BMI growth velocity, when compared to carriers of the minor allele (difference in velocity [95% CI]: 0.16/year [0.07-0.24] at age 10), which led to significantly higher average BMI by age 16 (average difference [95% CI]: 1.29 [0.22-2.37]). Rs10305420 CC and rs6923761 GG genotypes had higher HOMA2-IR (β [95% CI]: 1.19% [1.06-1.32] and 1.13% [1.01-1.26], respectively) compared to minor allele carriers. Rs10305420 CC had higher HOMA2-%B (β [95% CI]: 1.09% [1.01-1.17]), and higher stimulated insulin secretion at 30 minutes (β [95% CI]: 27.62 μIU/mL [3.00-25.24]) and 120 minutes (β [95% CI]: 18.94 μIU/mL [1.04-36.84]), when compared to carriers of the minor allele.

Conclusion
GLP-1R polymorphisms are associated with faster BMI growth across development, and lower estimated insulin sensitivity and higher compensatory insulin secretion during adolescence. GLP-1R polymorphisms should be considered in future pediatric studies of genetic susceptibility for obesity and diabetes.
Diagnosing Hypoparathyroidism, Sensorineural Deafness, and Renal Dysplasia Syndrome and a Novel GATA3 Variant
Authors: Valenciaga Anisley , Brock Pamela , O’Donnell Benjamin , Ing Steven W 
Published: December 2024

Abstract
Hypoparathyroidism (hypoPTH), sensorineural deafness, and renal dysplasia (HDR) syndrome is a rare autosomal dominant condition with approximately 200 cases published. HDR syndrome is caused by variants of GATA binding protein 3 gene (GATA3), which encodes a transcription factor, with multiple types of GATA3 variants reported. We present the case of a 76-year-old woman who was diagnosed with hypoPTH when she was aged 40 years and transferred care to our institution. Further history elucidated presence of deafness at age 1 year and chronic kidney disease with a left atrophic kidney diagnosed in her 60 seconds. Genetic testing identified a novel GATA3 missense variant of unknown significance (c.791G &gt; A, p.Cys264Tyr). There was no family history of hypoPTH, deafness, or renal disease, which might indicate incomplete penetrance or de novo mutation. Advanced modeling of protein sequence and biophysical properties predicts abnormal protein function, suggesting possible pathogenicity. In addition, a likely pathogenic variant in the same amino acid was previously described in a patient with HDR, supporting the in silico prediction of pathogenicity in our patient's variant. Syndromic hypoPTH should be considered in patients even if presenting later in life with presumed chronic isolated conditions. Genetic testing can guide further disease screening and family testing when appropriate.