bluuebunny/crossref_metadata_2025_split huggingface dataset:
A quick UPLC–MS/MS method for therapeutic drug monitoring of abiraterone and delta(4)‐abiraterone in human plasma
Authors: Weiss Thaís Luise Dillenburg , Furtado Carolina Mesquita , Antunes Marina Venzon , Gössling Gustavo , Schwartsmann Gilberto , Linden Rafael , Verza Simone Gasparin 
Published: July 2020

AbstractAbiraterone acetate efficacy against prostate cancer is dependent on the circulating levels of abiraterone and its active metabolites, which present significant pharmacokinetic variability among patients. Thus, therapeutic drug monitoring can be performed to improve treatment outcomes. To support such studies, there are only a limited number of bioanalytical methods in current literature. This work presents a fast method to quantify abiraterone and D4A in plasma in 4 min by UPLC–MS/MS. Bioanalytical method validation was performed according to the recommendations of the US Food and Drug Administration. The method was linear within the range of 1–400 ng/ml for abiraterone and 0.2–20 ng/ml for D4A (r2 > 0.99). Based on the analysis of quality control samples at the lower limit of quantification, low, medium and high concentrations, the method was precise (CVabiraterone ≤ 9.72%; CVD4A ≤ 14.64%) and accurate (CVabiraterone 95.51–107.59%; CVD4A 98.04–99.89%). Application of the method to the quantification of abiraterone and D4A in 10 clinical samples revealed important variability in the conversion ratio of abiraterone to D4A (CV 90.85%). Considering the current literature, this is the fastest method to quantify abiraterone and D4A in plasma, allowing for optimization of the analytical routine.
Appraisal of urgent transcatheter aortic valve replacement
Authors: Kornowski Ran 
Published: July 2020

Key Points
Urgent transcatheter aortic valve replacement (TAVR) is needed in unstable patients with severe aortic stenosis and acute decompensated heart failure who are refractory to initial medical management.
The outcome of urgent TAVR is worse and heavily influenced by the severity of the clinical status prior to intervention.
Careful patient evaluation, procedural planning, and postoperative management should be optimized to improve the clinical results.
Can percutaneous coronary interventions be performed safely in leukemia patients?
Authors: Grines Cindy L. , Cox David 
Published: July 2020

Key Points
Patients with leukemia are often denied percutaneous coronary intervention (PCI), and those who do undergo PCI are older and sicker.
There is a minor increase in vascular complications and no increase in retroperitoneal bleeds, suggesting transfusions may be given for reasons unrelated to the PCI.
Since cardiac complications are not increased in leukemia patients, the increased mortality observed in after PCI in acute leukemia may be related more to underlying malignancy.
Belt and suspenders: Why it pays to protect and cover during carotid stenting
Authors: Hering Dagmara , Heuser Richard R. 
Published: July 2020

Key Points
Carotid stenting results show equivalence to carotid endarterectomy.
Most patients undergoing carotid intervention are asymptomatic.
Using embolic protection with a covered mesh stent shows promise in this first large multicenter study.
State‐of‐the‐art percutaneous coronary interventions applied to the treatment of bifurcation lesions: Are we there yet?
Authors: Cheney Amy , Dean Larry S. 
Published: July 2020

Key Points
Treatment of bifurcation lesions has advanced with the development of second‐generation drug‐eluting stents and state‐of‐the‐art percutaneous coronary interventions techniques.
This subanalysis of SYNTAX II demonstrates similar major adverse cardiac or cerebrovascular events in patients with bifurcation and nonbifurcation lesions, with a trend toward higher target lesion failure in the bifurcation cohort.
The results, while compelling, require larger studies with longer follow‐up, stratified by bifurcation strategy.
Revascularization for multivessel disease: Getting closer, but…
Authors: Butman Samuel M. 
Published: July 2020

Key Points
In a registry, it is all about any differences.
PCI and CABG for multivessel disease are comparable with regard to risk of death/MI/stroke, but
PCI has a significantly higher risk of return to Cath Lab for PCI or even CABG in follow‐up.
Assessing atherothrombotic burden with optical coherence tomography analysis may facilitate post‐PCI prognostication and antiplatelet therapy
Authors: Costello Briana , Krajcer Zvonimir 
Published: July 2020

Key Points
Atherothrombotic burden (ATB) correlates with myocardial blush grade (MBG), a known predictor of mortality and outcome after ST‐elevation myocardial infarction.
Quantitative or semiquantitative analysis of ATB with optical coherence tomography may assist interventional cardiologists in post‐PCI prognostication and, perhaps, in rendering more aggressive antiplatelet therapy.
Future studies comparing treatment strategies for post‐PCI patients with elevated ATB or depressed MBG may identify best practices after acute coronary syndromes.
Morphine in primary percutaneous coronary intervention—No pain, questionable gain
Authors: Sridharan Aadhavi , Kimmelstiel Carey 
Published: July 2020

Key Points
The clinical impact of concurrently administered morphine and P2Y12 inhibitors in primary percutaneous coronary intervention (PCI) is not well understood.
Large, randomized clinical trials are required to assess clinical outcomes with concurrent morphine and P2Y12 inhibitor use in ST‐segment elevation myocardial infarction patients undergoing PCI.
Based on the currently available pharmacologic data, cautious use of morphine in primary PCI would seem prudent.
Recent Advances in Silver‐Mediated Radical Difunctionalization of Alkenes
Authors: Wu Yan‐Chen , Xiao Yu‐Ting , Yang Yong‐Zheng , Song Ren‐Jie , Li Jin‐Heng 
Published: July 2020

AbstractRadical‐initiated difunctionalization of alkenes is one of the most important methods in organic synthesis and medicinal chemistry, which can be applied to synthesize value complex compounds as well as structural motifs that found in bioactive natural products and pharmaceuticals. In recent years, impressive progress have been made in this area with ideal silver catalysis. Here, we summarize recent advances in silver‐mediated radical difunctionalization of alkenes for the formation of diverse bonds, including 1) two‐component radical difunctionalization reactions enabled by an intramolecular cyclization process toward various cyclic compounds and 2) three‐component radical difunctionalization reactions leading to complex linear compounds. These silver‐mediated radical alkene difunctionalization transformations are general initiated by different radicals, such as carbon‐, oxygen‐, sulfur‐, phosphinyl‐, and halogen‐center radicals, followed by terminated with nucleophiles to form two new bonds in a single reaction.
Tuning the Adsorption Selectivity of ZIF‐8 by Amorphization
Authors: Ma Qiang , Jin Hua , Li Yanshuo 
Published: July 2020

AbstractAmorphous metal–organic frameworks (amMOFs) with a partially collapsed structure are a new category of porous hybrid materials. Here, solid‐state amorphization of ZIF‐8 was achieved by mechanical compression at 0.75 GPa. The compression‐induced amorphous ZIF‐8 (amZIF‐8) had a collapsed structure, but retained partial porosity. Benefiting from the deformed channel, the resultant amZIF‐8 exhibited preferable adsorption of C3H6, resulting in higher thermodynamic adsorption selectivity of C3H6/C3H8 (6.72) than the crystalline counterparts (1.06). Further, amZIF‐8 achieved complete separation of an equimolar C3H6/C3H8 mixture with the first breakthrough of C3H8. amZIF‐8 also displayed an enhancement in CO2/N2 and CO2/CH4 adsorption selectivities. More importantly, a self‐standing amZIF‐8 membrane with boundary‐free microstructure was constructed for the first time, and exhibited separation potential for H2/CH4, CO2/N2, CO2/CH4, and C3H6/C3H8 with ideal selectivities of 14.79, 12.83, 16.23, and 2.67, respectively.
Photophysical Properties of 4‐Dicyanomethylene‐2‐methyl‐6‐(p‐dimethylamino‐styryl)‐4H‐pyran Revisited: Fluorescence versus Photoisomerization
Authors: Casimiro Lorenzo , Maisonneuve Stéphane , Retailleau Pascal , Silvi Serena , Xie Juan , Métivier Rémi 
Published: July 2020

AbstractAlthough 4‐dicyanomethylene‐2‐methyl‐6‐(p‐dimethylamino‐styryl)‐4H‐pyran (DCM) has been known for many decades as a bright and photostable fluorophore, used for a wide variety of applications in chemistry, biology and physics, only little attention has been paid so far to the presence of multiple isomers and conformers, namely s‐trans‐(E), s‐cis‐(E), s‐trans‐(Z), and s‐cis‐(Z). In particular, light‐induced E–Z isomerization plays a great role on the overall photophysical properties of DCM. Herein, we give a full description of a photoswitchable DCM derivative by a combination of structural, theoretical and spectroscopic methods. The main s‐trans‐(E) isomer is responsible for most of the fluorescence features, whereas the s‐cis‐(E) conformer only contributes marginally. The non‐emitting Z isomers are generated in large conversion yields upon illumination with visible light (e.g., 485 or 514 nm) and converted back to the E forms by UV irradiation (e.g., 365 nm). Such photoswitching is efficient and reversible, with high fatigue resistance. The E→Z and Z→E photoisomerization quantum yields were determined in different solvents and at different irradiation wavelengths. Interestingly, the fluorescence and photoisomerization properties are strongly influenced by the solvent polarity: the fluorescence is predominant at higher polarity, whereas photoisomerization becomes more efficient at lower polarity. Intermediate medium (THF) represents an optimized situation with a good balance between these two features.
Controlling Mirror Symmetry Breaking and Network Formation in Liquid Crystalline Cubic, Isotropic Liquid and Crystalline Phases of Benzil‐Based Polycatenars
Authors: Reppe Tino , Poppe Silvio , Tschierske Carsten 
Published: July 2020

AbstractSpontaneous development of chirality in systems composed of achiral molecules is important for new routes to asymmetric synthesis, chiral superstructures and materials, as well as for the understanding of the mechanisms of emergence of prebiotic chirality. Herein, it is shown that the 4,4′‐diphenylbenzil unit is a universal transiently chiral bent building block for the design of multi‐chained (polycatenar) rod‐like molecules capable of forming a wide variety of helically twisted network structures in the liquid, the liquid crystalline (LC) and the crystalline state. Single polar substituents at the apex of tricatenar molecules support the formation of the achiral (racemic) cubic double network phase with Ia
d symmetry and relatively small twist along the networks. The combination of an alkyl chain with fluorine substitution leads to the homogeneously chiral triple network phase with I23 space group, and in addition, provides a mirror symmetry broken liquid. Replacing F by Cl or Br further increases the twist, leading to a short pitch double gyroid Ia
d phase, which is achiral again. The effects of the structural variations on the network structures, either leading to achiral phases or chiral conglomerates are analyzed.
Anchoring BaII to Pd/HyWO3−x Nanowires Promotes a Photocatalytic Reverse Water–Gas Shift Reaction
Authors: Li Young Feng , Lu Waylon , Chen Kai , Xia Meikun , Feysal Ali M. , Ozin Geoffrey A. 
Published: July 2020

AbstractSurface deposition of BaII on Pd/HyWO3−x nanowires was developed by using a solution‐phase atomic layer deposition process. The procedure involves the generation of Brønsted surface OH sites by H2 spillover on Pd/WO3, which can then hydrolytically condense with Ba(OEt)2 to produce surface BaII. At just 0.2 at % Ba, CO production by the light‐assisted activity of the reverse water–gas shift (RWGS) reaction was observed to increase by about 300 %. In situ DRIFTS studies suggested enhanced CO2 capturing capabilities of a Ba‐decorated surface. This study further exemplifies the importance of surface chemistry in optimizing materials for catalysis.
The Impact of Leaving Group Anomericity on the Structure of Glycosyl Cations of Protected Galactosides
Authors: Greis Kim , Mucha Eike , Lettow Maike , Thomas Daniel A. , Kirschbaum Carla , Moon Sooyeon , Pardo‐Vargas Alonso , von Helden Gert , Meijer Gerard , Gilmore Kerry , Seeberger Peter H. , Pagel Kevin 
Published: July 2020

AbstractIt has been reported that fragments produced by glycosidic bond breakage in mass spectrometry‐based experiments can retain a memory of their anomeric configuration, which has major implications for glycan sequencing. Herein, we use cryogenic vibrational spectroscopy and ion mobility‐mass spectrometry to study the structure of B‐type fragments of protected galactosides. Cationic fragments were generated from glycosyl donors carrying trichloroacetimidate or thioethyl leaving groups of different anomeric configuration. The obtained infrared signatures indicate that the investigated fragments exhibit an identical structure, which suggests that there is no anomeric memory in B‐type ions of fully protected monosaccharides.
Mandibular undifferentiated pleomorphic sarcoma: Molecular analysis of a primary cell population
Authors: Amm Hope M. , DeVilliers Patricia , Srivastava Ambika R. , Diniz Marina G. , Siegal Gene P. , MacDougall Mary 
Published: July 2020

AbstractBackgroundUndifferentiated pleomorphic sarcomas are one of the most common subtypes of soft tissue sarcomas. These are aggressive mesenchymal tumors and are devoid of the major known biomarkers except vimentin. Our objective was to establish and characterize a primary cell population from a mandibular UPS specimen.MethodsThe tumor was surgically removed from the right mandible of a 24‐year‐old male with IRB approved signed consent. Tumor was dissected, cultured ex vivo, and a cell population, MUPS‐1, were isolated from outgrowths. Gene and protein expression profiles of both the primary tumor and the derived there from cells were obtained by quantitative RT‐PCR and immunohistochemistry and included markers of epithelial, endothelial, and mesenchymal differentiation. To better define potential biomarkers, MUPS‐1 cells were additionally characterized by RNA sequencing analysis.ResultsPathological analysis of primary tumor tissue revealed a sarcoma demonstrating multiple pathways of differentiation simultaneously with myxoid, fibrous, and osseous tissue. The isolated cells had a spindle cell‐like morphology, were maintained in culture for greater than 20 passages, and formed colonies in soft agar indicating tumorigenicity. The cells, similar to the primary tumor, were strongly positive for vimentin and moderately expressed alkaline phosphatase. RNA‐seq analysis revealed the tumor over‐expressed several genes compared to normal tissue, including components of the Notch signaling pathway, NOTCH3 and JAG1.ConclusionsWe have successfully established an undifferentiated pleomorphic sarcoma cell population, which will provide a valuable resource for studying fundamental processes and potentially serving as a platform for exploring therapeutic strategies for sarcomas.
Complications of cutaneous surgery
Authors: Rothstein Brooke E. , Cohen Joel L. 
Published: July 2020

AbstractBackgroundCutaneous surgery is associated with multiple complications including hemorrhage, surgical site infection, necrosis, and scarring. As demand for dermatologic surgery rises, it is imperative that dermatologists have a comprehensive understanding of the potential surgical risks to enhance patient safety and outcomes.AimsThe purpose of this review is to highlight the rates, prevention, and management of common complications associated with cutaneous surgery, with a focus on scalpel‐based surgeries including Mohs micrographic surgery and standard surgical excision.Material and MethodsArticles from PubMed are highlighted and our clinical experience is presented.ResultsCutaneous surgery is overall safe and associated with low rates of complications that are predominantly minor and not life‐threatening. Prevention and management strategies vary according to the specific complication being addressed.DiscussionDermatologists should be familiar with the complications of cutaneous surgery. As our knowledge of surgical complications expands, the outcomes of our patients will continue to improve.
Stimulations inducing tethered lipid bilayer formation on protein‐modified substrate
Authors: Tero Ryugo , Sato Junichi 
Published: July 2020

AbstractSupported lipid bilayer (SLB) is one artificial biomembrane system used to study transportation reactions through biomembranes using solid devices and surface scientific methods. A key step in the SLB formation is the transformation of spherical lipid vesicles to a planar membrane. We investigated stimulation methods for adsorbed lipid vesicles on a streptavidin‐modified SiO2/Si substrate to induce tethered‐type SLB. We describe the effects of osmotic stress, Ca2+ addition, and a freeze–thaw cycle on the adsorbed vesicles, and on the condition of t‐SLB.
Bcl‐3 inhibits lupus‐like phenotypes in BL6/lpr mice
Authors: Tang Wanhu , Wang Hongshan , Tian Ruxiao , Saret Sun , Cheon HeeJin , Claudio Estefania , Siebenlist Ulrich 
Published: July 2020

AbstractBcl‐3 is an atypical member of the IκB family that modulates NF‐κB activity in nuclei. lpr mice carry the lpr mutation in Fas, resulting in functional loss of this death receptor; they serve as models for lupus erythematosus and autoimmune lymphoproliferation syndrome (ALPS). To explore the biologic roles of Bcl‐3 in this disease model, we generated BL6/lpr mice lacking Bcl‐3. Unlike lpr mice on an MRL background, BL6/lpr mice present with very mild lupus‐ or ALPS‐like phenotypes. Bcl‐3 KO BL6/lpr mice, however, developed severe splenomegaly, dramatically increased numbers of double negative T cells — a hallmark of human lupus, ALPS, and MRL/lpr mice — and exhibited inflammation in multiple organs, despite low levels of autoantibodies, similar to those in BL6/lpr mice. Loss of Bcl‐3 specifically in T cells exacerbated select lupus‐like phenotypes, specifically organ infiltration. Mechanistically, elevated levels of Tnfα in Bcl‐3 KO BL6/lpr mice may promote lupus‐like phenotypes, since loss of Tnfα in these mice reversed the pathology due to loss of Bcl‐3. Contrary to the inhibitory functions of Bcl‐3 revealed here, this regulator has also been shown to promote inflammation in different settings. Our findings highlight the profound, yet highly context‐dependent roles of Bcl‐3 in the development of inflammation‐associated pathology.
Functionally distinct IFN‐γ+IL‐17A+ Th cells in experimental autoimmune uveitis: T‐cell heterogeneity, migration, and steroid response
Authors: Chen Yi‐Hsing , Eskandarpour Malihe , Gondrand Aurelia , Zhang Xiaozhe , Gu Renyang , Galatowicz Grazyna , Lightman Sue L. , Calder Virginia L. 
Published: July 2020

AbstractImmunopathogenic roles for both Th1 (CD4+IFN‐γ+) and Th17 (CD4+IL‐17A+) cells have been demonstrated in experimental autoimmune uveitis (EAU). However, the role for Th17/Th1 (CD4+ T cells co‐expressing IFN‐γ and IL‐17A) cells in EAU is not yet understood. Using interphotoreceptor retinoid‐binding protein peptide‐induced EAU in mice, we found increased levels of Th17/Th1 cells in EAU retinae (mean 9.6 ± 4.2%) and draining LNs (mean 8.4 ± 3.9%; p = 0.01) relative to controls. Topical dexamethasone treatment effectively reduced EAU severity and decreased retinal Th1 cells (p = 0.01), but had no impact on retinal Th17/Th1 or Th17 cells compared to saline controls. Using in vitro migration assays with mouse CNS endothelium, we demonstrated that Th17/Th1 cells were significantly increased within the migrated population relative to controls (mean 15.6 ± 9.5% vs. 1.9 ± 1.5%; p = 0.01). Chemokine receptor profiles of Th17/Th1 cells (CXCR3 and CCR6) did not change throughout the transendothelial migration process and were unaffected by dexamethasone treatment. These findings support a role for Th17/Th1 cells in EAU and their resistance to steroid inhibition suggests the importance of targeting both Th17 and Th17/Th1 cells for improving therapy.
Effect of the application of an edible film with turmeric (Curcuma longa L.) on the oxidative stability of meat
Authors: Bojorges Hylenne , Ríos‐Corripio M. A. , Hernández‐Cázares Aleida S. , Hidalgo‐Contreras Juan Valente , Contreras‐Oliva Adriana 
Published: July 2020

AbstractThe aim of this study was to develop an edible alginate‐based film produced with turmeric (EFT), as an active compound, and evaluate its antioxidant capacity for application in fresh pork loin, beef loin, and chicken breast. The EFT was characterized by barrier parameters, color, and mechanical, structural, and antioxidant properties. Meat samples with and without EFT were stored at 4°C and analyzed at 2‐day intervals. The meat samples with EFT showed significant differences (p < .05) in color (CIE L*a*b*) and exhibited lower TBARS values compared with those without EFT. The addition of turmeric in the film, besides affecting its physicochemical and structural properties, contributed an important antioxidant effect for the meat.
Functional connectome fingerprinting accuracy in youths and adults is similar when examined on the same day and 1.5‐years apart
Authors: Jalbrzikowski Maria , Liu Fuchen , Foran William , Klei Lambertus , Calabro Finnegan J. , Roeder Kathryn , Devlin Bernie , Luna Beatriz 
Published: July 2020

AbstractPioneering studies have shown that individual correlation measures from resting‐state functional magnetic resonance imaging studies can identify another scan from that same individual. This method is known as “connectotyping” or functional connectome “fingerprinting.” We analyzed a unique dataset of 12–30 years old (N= 140) individuals who had two distinct resting state scans on the same day and again 12–18 months later to assess the sensitivity and specificity of fingerprinting accuracy across different time scales (same day, ~1.5 years apart) and developmental periods (youths, adults). Sensitivity and specificity to identify one's own scan was high (average AUC = 0.94), although it was significantly higher in the same day (average AUC = 0.97) than 1.5‐years later (average AUC = 0.91). Accuracy in youths (average AUC = 0.93) was not significantly different from adults (average AUC = 0.96). Multiple statistical methods revealed select connections from the Frontoparietal, Default, and Dorsal Attention networks enhanced the ability to identify an individual. Identification of these features generalized across datasets and improved fingerprinting accuracy in a longitudinal replication data set (N= 208). These results provide a framework for understanding the sensitivity and specificity of fingerprinting accuracy in adolescents and adults at multiple time scales. Importantly, distinct features of one's “fingerprint” contribute to one's uniqueness, suggesting that cognitive and default networks play a primary role in the individualization of one's connectome.