bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gastrointestinal nematodes of European wild boar from distinct agricultural and forest habitats in Poland
Authors: Nosal Paweł , Kowal Jerzy , Wyrobisz-Papiewska Anna , Wajdzik Marek 
Published: February 2020

AbstractThe study aimed to compare the gastrointestinal helminthofauna of free-ranging wild boars from arable lands and forests, which are the natural habitats for wild boar in Poland and further to investigate if wild boars living in agricultural environments could acquire helminths commonly detected in domestic pigs. In 2011–2014, a total of 57 wild boars were examined post-mortem for the presence of gastrointestinal nematodes. Altogether, all but two of the animals were infected, and seven nematode species were found. The mean infection burden was 68.9 parasites, ranging from 1 to 381 worms. In forest areas, Ascarops strongylina, Physocephalus sexalatus, and Globocephalus urosubulatus were common, whereas on arable lands, the animals were more frequently infected (P < 0.05) by Ascaris suum and Trichuris suis, which are parasites that commonly occur in domestic pigs. Oesophagostomum dentatum was observed only in wild boars on arable lands, and Bourgelatia diducta, which is alien to European suids, appeared irrespective of habitat type. These results show significant differences in parasite spectra among wild boars living in forests or arable lands in Poland and indicates the risks of parasite transfer from domestic pigs to free-ranging wild boars. Furthermore, in farmed game, organic farming, or in the case of agritourism farms, one should be aware of the risk of related animals acquiring new and alien parasite infections by being kept outdoors.
Considerations on the taxonomy and morphology of Microcotyle spp.: redescription of M. erythrini van Beneden & Hesse, 1863 (sensu stricto) (Monogenea: Microcotylidae) and the description of a new species from Dentex dentex (L.) (Teleostei: Sparidae)
Authors: Víllora-Montero María , Pérez-del-Olmo Ana , Georgieva Simona , Raga Juan Antonio , Montero Francisco Esteban 
Published: February 2020

AbstractBackgroundMicrocotyle erythrinivan Beneden & Hesse, 1863 (Platyhelminthes: Monogenea) and other closely related species of the genus are often considered as cryptic. Records in hosts other than the type-host with no species confirmation by molecular analyses have contributed to this situation.MethodsGill parasites of five sparid fishes,Boops boops(L.),Pagellus erythrinus(L.),P. acarne(Risso), Dentex dentex(L.) andPagrus pagrus(L.), from the Western Mediterranean off Spain were collected. Specimens ofMicrocotylespp. were characterised both molecularly and morphologically. Partial fragments (domains D1-D3) of the28SrRNA gene and the cytochromecoxidase subunit 1 (cox1) gene were amplified and used for molecular identification and phylogenetic reconstruction. Principal components analysis was used to look for patterns of morphological separation.ResultsMolecular analyses confirmed the identity of three species:M. erythriniexP. erythrinusandPa. pagrus;M. isyebiBouguerche, Gey, Justine & Tazerouti, 2019 exB. boops; and a species new to science described herein,M. whittingtonin. sp. exD. dentex.The specific morphological traits and confirmed hosts (P. erythrinusandPa. pagrus) are delimited here in order to avoid misidentifications ofM. erythrini(sensu stricto).Microcotyle erythrini(s.s.) is mostly differentiated by the shape of its haptor, which is also longer than in the other congeners. New morphological and molecular data are provided forM. isyebifrom the Spanish Mediterranean enlarging the data on its geographical range.Microcotyle whittingtonin. sp. is described fromD. dentexand distinguished from the remaining currently recognised species of the genus by the number and robustness of the clamps.ConclusionsNew diagnostic morphological traits useful to differentiateMicrocotylespp. are suggested: (i) haptor dimensions including lobes; (ii) the thickness of the clamps; (iii) the size and shape of spines of the genital atrium; (iv) the extension of the posterior extremities of vitelline fields; and (v) the shape of egg filaments. The use of new morphological approaches may allow considering these species ofMicrocotyleas being pseudocryptic. The use of representative undamaged specimens that have been genetically confirmed as conspecific is considered crucial to avoid abnormally wide morphological ranges that prevent species differentiation.
Treating medically unexplained symptoms via improving access to psychological therapy (IAPT): major limitations identified
Authors: Geraghty Keith , Scott Michael J. 
Published: February 2020

AbstractBackgroundImproving Access to Psychological Therapies is a UK Government funded initiative to widen access to psychological treatment for a range of common mental health complaints, such as depression and anxiety. More recently, the service has begun to treat patients with medically unexplained symptoms. This paper reports on a review of treatment protocols and early treatment data for medically unexplained symptoms, specifically the illness myalgic encephalomyelitis/chronic fatigue syndrome.Main textA series of seven core problems and failings are identified, including an unproven treatment rationale, a weak and contested evidence-base, biases in treatment promotion, exaggeration of recovery claims, under-reporting of drop-out rates, and a significant risk of misdiagnosis and inappropriate treatment.ConclusionsThere is a pressing need for independent oversight of this service, specifically evaluation of service performance and methods used to collect and report treatment outcomes. This service offers uniform psycho-behavioural therapy that may not meet the needs of many patients with medically unexplained health complaints. Psychotherapy should not become a default when patients’ physical symptoms remain unexplained, and patients should be fully informed of the rationale behind psychotherapy, before agreeing to take part. Patients who reject psychotherapy or do not meet selection criteria should be offered appropriate medical and psychological support.
H3F3A mutant allele specific imbalance in an aggressive subtype of diffuse midline glioma, H3 K27M-mutant
Authors: Maeda Sachi , Ohka Fumiharu , Okuno Yusuke , Aoki Kosuke , Motomura Kazuya , Takeuchi Kazuhito , Kusakari Hironao , Yanagisawa Nobuyuki , Sato Shinya , Yamaguchi Junya , Tanahashi Kuniaki , Hirano Masaki , Kato Akira , Shimizu Hiroyuki , Kitano Yotaro , Yamazaki Shintaro , Yamashita Shinji , Takeshima Hideo , Shinjo Keiko , Kondo Yutaka , Wakabayashi Toshihiko , Natsume Atsushi 
Published: February 2020

AbstractDiffuse midline glioma, H3 K27M-mutant is a lethal brain tumor located in the thalamus, brain stem, or spinal cord. H3 K27M encoded by the mutation of a histone H3 gene such as H3F3A plays a pivotal role in the tumorigenesis of this type of glioma. Although several studies have revealed comprehensive genetic and epigenetic profiling, the prognostic factors of these tumors have not been identified to date. In various cancers, oncogenic driver genes have been found to exhibit characteristic copy number alterations termed mutant allele specific imbalance (MASI). Here, we showed that several diffuse midline glioma, H3 K27M-mutant exhibited high variant allele frequency (VAF) of the mutated H3F3A gene using droplet digital polymerase chain reaction (ddPCR) assays. Whole-genome sequencing (WGS) revealed that these cases had various copy number alterations that affected the mutant and/or wild-type alleles of the H3F3A gene. We also found that these MASI cases showed a significantly higher Ki-67 index and poorer survival compared with those in the lower VAF cases (P < 0.05). Our results indicated that the MASI of the H3F3A K27M mutation was associated with the aggressive phenotype of the diffuse midline glioma, H3 K27M-mutant via upregulation of the H3 K27M mutant protein, resulting in downregulation of H3K27me3 modification.
Thermophysical properties of surficial rocks: a tool to characterize geothermal resources of remote northern regions
Authors: Miranda M. M. , Giordano N. , Raymond J. , Pereira A. J. S. C. , Dezayes C. 
Published: February 2020

AbstractThe energetic framework of Canadian remote communities relies on fossil fuels. This has adverse environmental and energy security issues. In order to offset diesel consumption, the search for local, sustainable and carbon-free energy sources is of utmost importance. Unfortunately, in such remote regions, subsurface data to evaluate the geothermal potential is often nonexistent. This raises a key question: how to characterize geothermal resources associated to petrothermal systems based on surface data? Answering this question is the purpose of this work highlighting how outcrops can be used as deep subsurface analogues. The variability induced by laboratory methods to characterize thermophysical properties is further evaluated in the estimation of the present-day temperature at depth. The community of Kuujjuaq, Canada, is used as an example where guidelines are defined to evaluate the steady-state geotherm. Rock samples were collected and analyzed with a guarded heat flow meter and an optical scanner to determine thermal conductivity. Radiogenic elements concentration was evaluated with gamma-ray and mass spectrometry. 2D temperature models were built taking into account the regional geology and the results obtained from the different laboratory methods. A base-case temperature of 57–88 °C at 5 km is predicted below Kuujjuaq. This range is based on different methods used to evaluate both thermal conductivity and internal heat generation. The work conducted in Kuujjuaq shows that the combination of gamma-ray spectrometry and optical scanning gives lower base-case temperature predictions when compared to mass spectrometry combined with the guarded heat flow meter. Despite the nonexistence of deep temperature measurements in northern regions, the assessment of thermophysical properties from outcrops is shown to be a useful tool for a preliminary assessment of geothermal resources in remote areas facing critical energy issues.
Initial implementation of active learning strategies in large, lecture STEM courses: lessons learned from a multi-institutional, interdisciplinary STEM faculty development program
Authors: Borda Emily , Schumacher Emily , Hanley Daniel , Geary Edward , Warren Shannon , Ipsen Cassidy , Stredicke Lauren 
Published: February 2020

Abstract
Background
A faculty development program was implemented over four years at a 4-year regional comprehensive university and two partnering community colleges. This project was focused on improving student learning in introductory Science, Technology, Engineering, and Math (STEM) courses at each institution, by helping faculty adopt inclusive, student-centered pedagogies. Survey data were combined with data from classroom videos, faculty interviews, and student questionnaires collected during the first two years of the project to give insight into how faculty initially implemented the theory and strategies they learned and how the students perceived instruction from participating faculty.

Results
These data sources were combined to generate four overall themes to characterize our project and guide future projects. These are: (1) implementation of student-centered learning took a variety of forms; (2) quality implementation of student-centered teaching practices lagged behind understanding of the theory behind those practices; (3) the most robust perceived barriers to implementation of student-centered teaching stayed constant, while more moderate barriers were ranked differently from year 1 to year 2; and (4) faculty perceptions of student-centered learning practices were not always the same as students’ perceptions. These themes build from the extant faculty development literature in that they are drawn from the unique context of a multidisciplinary, multi-institutional project, and that they represent an “on the ground” perspective from case studies combined with “big picture” findings from surveys.

Conclusions
This paper describes the faculty development project, as well as our collection and interpretation of data from surveys and case studies, to ultimately develop the four themes. Recommendations deriving from these themes are also described. These include modeling a variety of pedagogies; adopting realistic expectations for faculty change; institutionalizing faculty development so it can take place over multiple years; being transparent with faculty about known barriers and aligning supports with those barriers; and helping faculty develop strategies for transparency with students about student-centered pedagogies.
Meningioma in mature cystic teratoma of the ovary: clinical and computed tomography findings
Authors: Liu Yi-yang , Liang Pan , Ji Jiang , Chen Kui-sheng , Li Li-ming , Gao Jian-bo , Yong Liu-liang 
Published: February 2020

Abstract
Background
Mature cystic teratoma (MCT) with meningioma of the ovary is a very rare benign tumor. There is only 3 reports of this disease until June 2019. The aim of the present study was to describe a ovarian mature cystic teratoma containing meningioma and nests of neuroblasts in a 15-year-old girl.

Methods
The method used in the present study consists of description of the clinical history, image lab features, and pathological result.

Results
The patient complained of a 2-month history of irregular vaginal bleeding. Abdominal computed tomography (CT) showed a large oval cystic-solid mass with septations and fat density shadow, in abdomen pelvic cavity. The cystic part was the main component in the mass. The tumoral solid parts and its internal division could be seen intensified from slight to moderate on contrast-enhanced CT images compared with those on precontrast images, and the solid parts showed heterogeneous enhancement. Neighbouring intestinal tract and the uterus displaced by compression. The pathological examination confirmed the diagnosis.

Conclusions
The clinical feature of ovarian mature cystic teratoma with meningioma includes a lack of specificity. Only meticulous recording of the gross features, histopathological examination including immunohistochemistry and supportive clinical and radiological findings to arrive at a correct diagnosis in case of unconventional tumours. If necessary, preoperative puncture can be performed.
Foraging behavior of the Greater White-fronted Goose (Anser albifrons) wintering at Shengjin Lake: diet shifts and habitat use
Authors: Fan Yanguang , Zhou Lizhi , Cheng Lei , Song Yunwei , Xu Wenbin 
Published: February 2020

Abstract
Background
The habitat use and foraging behaviors of waterbirds are closely related to the distribution and abundance of their food resources. Reductions in food supply can cause waterbirds to shift their habitats and adjust their foraging behaviors to meet their nutritional requirements and increase fitness. Seasonal withdraw of the water levels in the river-connected lakes in the middle and lower Yangtze River floodplain provides abundant food resources for the wintering Greater White-fronted Goose (Anser albifrons). Sedge (Carex) meadows are critical foraging habitats for herbivorous waterbirds in the hydro-fluctuation belt, which changes with hydrological conditions and climate. This study aimed to examine the behavioral responses of the Greater White-fronted Goose to temporal- spatial changes of food availability in the Sedge meadows.

Methods
Fields surveys were carried out at Shengjin Lake from November 2017 to April 2018. According to the phenology of Shengjin Lake, we divided the wintering season into three periods. The food density, minimum temperature, food items, grass height, and number of foraging geese were surveyed, and samples of the foraging behavior were collected. We analyzed the relationship of the foraging behavior and habitat use relative to the food resources, using correlation and linear regression analyses.

Results
Along with the temporal-spatial variation and exploitation of food resources, the food abundance and items varied widely among the foraging sites. Over the whole wintering period, the foraging habitat with the highest utilization rate was the meadows, followed by the paddy fields, and then mudflats. Furthermore, the utilization of the meadows showed a bimodal distribution trend, while the paddy fields showed a unimodal trend, and a decreasing trend was seen in the mudflats over the whole wintering period. The results of the generalized linear model showed that the foraging rate was related to the food density and grass height, with a linearly increasing trend during the winter.

Conclusions
With the change of food resources in the three habitats, the habitats used by the Greater White-fronted Geese shifted from meadows in the hydro-fluctuation belt to the paddy fields, and then back to the meadows. The time budget for foraging activities increased correspondingly, and there was an increase in the foraging rate to compensate for food shortages.
Categorization of lower body shapes of abdominal obese men using a script-based 3D body measurement software
Authors: Lee Kyu Sun , Song Hwa Kyung , Kim Sungmin 
Published: February 2020

AbstractThe objectives of this study are to identify the principal components that represent distinctive shapes from the silhouette and profile views of the lower body shapes of abdominal obese Korean men and to categorize their body types. Using 3D scans of 625 men aged 35–64 in the 6th SizeKorea dataset, 173 scans (27.7%) of men in ‘abdominal obese’ category (BMI value of 25, waist girth to height ratio of 0.53, and waist girth to hip girth ratio of 0.9 or higher) were utilized. We developed a script to measure 38 items such as front/back crotch length and front/back depths and angles using the SNU-BM program, which is a script-based automated 3D body scan measurement software. The measurements used for principal component (PC) analysis were 31 drops, 2 heights, 2 lengths and 4 angles. Ten PCs representing distinctive silhouettes and profiles of lower body shapes were extracted. The PCs were interpreted as follows: abdomen prominence, thigh to knee profile, upper buttocks prominence, waist to hip drop, thigh to knee silhouette, lower body tilt angle, waist to crotch length, vertical height, abdomen to crotch height, and lower buttocks slope. The three body shape groups were categorized using a K means cluster analysis with ten PC scores. Group 1 had a flat abdomen but prominent buttocks. Group 2 had a developed abdomen and buttocks with vertical thighs. Group 3 had drooped buttocks with tilted thighs.
A new redescription of Richtersius coronifer, supported by transcriptome, provides resources for describing concealed species diversity within the monotypic genus Richtersius (Eutardigrada)
Authors: Stec Daniel , Krzywański Łukasz , Arakawa Kazuharu , Michalczyk Łukasz 
Published: February 2020

AbstractRichtersius coronifer, the nominal species for the family Richtersiidae and a popular laboratory model, exemplifies a common problem in modern tardigrade taxonomy. Despite undeniable progress in the field, many old and incomplete descriptions of taxa hinder both species delimitation and the estimation of species diversity and distribution. Although for over a century this species has been recorded throughout the world, recent research indicates that records to date are likely to represent a species complex rather than a single cosmopolitan species. However, in order to recognise and name species diversity within the complex, an integrative redescription of the nominal species is first needed. Here, we describe an R. coronifer population collected from Spitsbergen, i.e., one of the two localities mentioned in the original description, with detailed morphological and morphometric data associated with standard DNA sequences of four standard genetic markers (18S rRNA, 28S rRNA, ITS-2, and COI) and supported by transcriptome sequencing. We propose replacement of the neotype designated in 1981 by Maucci and Ramazzotti, as it is impossible to verify whether the existing neotype is conspecific with specimens studied by Richters in 1903 and 1904. Finally, using newly obtained cytochrome c oxidase subunit I (COI) sequences of populations from Spitsbergen, Italy, Poland, and Greece together with sequences deposited in GenBank (China, Greenland, Italy, Mongolia), we performed genetic species delimitation, which indicated seven distinct potential species within the genus Richtersius, in addition to the nominal taxon. This study marks a starting point for further research on the taxonomy of and species diversity within the genus. Moreover, this work has the potential to be the first tardigrade redescription to provide both genetic barcodes and a transcriptome of the species in question.
Nicotine attenuates global genomic DNA methylation by influencing DNMTs gene expression in human endometrial stromal cells
Authors: Zal Fatemeh , Yarahmadi Amir , Totonchi Hamidreza , Barazesh Mahdi , Moradi Sarabi Mostafa 
Published: February 2020

Abstract
Background
There is increasing evidence indicating an incidence of infertility and also the risk of endometrial cancers among smokers. However, the mechanism underlying nicotine adverse effect on female reproduction remains unclear. Growing evidence has suggested that environmental exposures such as nicotine could modulate the epigenome. No study has yet been published to evaluate the direct effect of nicotine on the epigenome profiling of human endometrial stromal cells (HESC). Herein, we decided to examine the direct effects of nicotine on global genomic DNA methylation status and DNA methyl- transferases (DNMTs) gene expression in HESC. HESC were treated with different doses of nicotine (0 or control, 10− 11, 10− 8 and 10− 6) M for 24 h and their genomic global DNA methylation and gene expression of DNMTs (DNMT1, DNMT3A, and DNMT3B) were investigated using ELISA and real-time PCR, respectively.

Results
Nicotine treatments reduced the average level of DNMTs gene expression by 90, 79, and 73.4% in 10− 11, 10− 8 and 10− 6 M of nicotine treated cells as compared to control cells, respectively (p < 0.05). Also, 10− 8 and 10− 6 M of nicotine concentrations effectively reduced the amounts of 5-methylated cytosine (5-mC) by 1.09 and 1.87% compared to control cells, respectively (p < 0.05). The 5-mC percentages were positively correlated with the relative cellular DNMTs expression in HESC as verified by the Pearson correlation test.

Conclusion
An interesting possibility raised by the current study is that the reduced genomic global DNA methylation level in HESC may be partly due to the suppression of DNMTs gene expression caused by nicotine in these cells.

Graphical abstract