bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Systems Model of Repeat Court-Ordered Removals: Responding to Child Protection Challenges Using a Systems Approach
Authors: Wise Sarah 
Published: March 2020

Abstract
Repeat removals, where parents lose successive infants and children to out-of-home care, is a systems problem requiring a systems approach to broaden our thinking and expand our choices for action. A transdisciplinary group of key stakeholders in Australia jointly constructed a causal loop diagram to bring forth the systemic structure underlying the issue and identify system conditions that need to be altered. Analysis of the causal loop diagram surfaced eight dynamic positive feedback loops which result in a self-reinforcing system. While the model reflects the understanding of a relatively small group and requires further testing, it adds to current conceptualisations of repeat removal by hypothesising systemic causes and offers new directions for prevention. Stakeholders homed in on stigmatised stereotypes of recurrent birth mothers and the child protection culture, practitioner decision-making, parent–practitioner interactions and parental participation in child protection case planning as ways of correcting against vicious feedback loops. A systems approach contributed useful insights into the problem of repeat removals and could productively be used in respect of a range of other complex child protection challenges.
Nebulized heparin for inhalation injury in burn patients: a systematic review and meta-analysis
Authors: Lan Xiaodong , Huang Zhiyong , Tan Ziming , Huang Zhenjia , Wang Dehuai , Huang Yuesheng 
Published: March 2020

AbstractBackgroundSmoke inhalation injury increases overall burn mortality. Locally applied heparin attenuates lung injury in burn animal models of smoke inhalation. It is uncertain whether local treatment of heparin is benefit for burn patients with inhalation trauma. We systematically reviewed published clinical trial data to evaluate the effectiveness of nebulized heparin in treating burn patients with inhalation injury.MethodsA systematic search was undertaken in PubMed, the Cochrane Library, Embase, Web of Science, the Chinese Journals Full-text Database, the China Biomedical Literature Database and the Wanfang Database to obtain clinical controlled trails evaluating nebulized heparin in the treatment of burn patients with inhalation injury. Patient and clinical characteristics, interventions and physiological and clinical outcomes were recorded. Cochrane Risk of Bias Evaluation Tool and the Newcastle–Ottawa Scale were used to evaluate data quality. Potential publication bias was assessed by Egger’s test. A sensitivity analysis was conducted to assess the stability of the results. The meta-analysis was conducted in R 3.5.1 software.ResultsNine trials were eligible for the systematic review and meta-analysis. Nebulized heparin can reduce lung injury and improve lung function in burn patients with inhalation injury without abnormal coagulation or bleeding, but the findings are still controversial. Mortality in the heparin-treated group was lower than that of the traditional treatment group (relative risk (RR) 0.75). The duration of mechanical ventilation (DOMV) was shorter in the heparin-treated group compared to the traditional treatment group (standardized mean difference (SMD) −0.78). Length of hospital stay was significantly shorter than that in the traditional treatment group (SMD −0.42), but incidence rates of pneumonia and unplanned reintubation were not significantly different in the study groups (RRs 0.97 and 0.88, respectively). No statistically significant publication biases were detected for the above clinical endpoints (p &gt; 0.05).ConclusionsBased on conventional aerosol therapy, heparin nebulization can further reduce lung injury, improve lung function, shorten DOMV and length of hospital stay, and reduce mortality, although it does not reduce the incidence of pneumonia and/or the unplanned reintubation rate.
Measuring and validating concentrations of steroid hormones in the skin of bottlenose dolphins (Tursiops truncatus)
Authors: Bechshoft Thea , Wright Andrew J , Styrishave Bjarne , Houser Dorian 
Published: March 2020

AbstractA previously published analytical method demonstrated the quantification of the hormone cortisol in cetacean skin. However, little is known about the transfer of hormones between blood and skin. Recognizing that such information is essential to effectively using skin samples within marine mammal stress research, the primary goals of this study were to (i) expand on the number of steroid hormones proved quantifiable in the cetacean skin matrix and (ii) validate the use of cetacean skin as a matrix for measuring stress-related hormones. Five adult bottlenose dolphins were subjected to an out of water stress test. Non-invasive sloughed skin samples were collected from each dolphin: once ~3 and once ~1 week prior to the stress test; at the time of the stress test; and twice weekly for 11 to 17 weeks subsequent to the stress test. LCMS/MS analysis of the samples recovered consistent data on three corticosteroids (cortisol, aldosterone, corticosterone), two androgens (testosterone, DHEA) and one progestagen (progesterone). A range of other hormones were also quantifiable, although not consistently so across samples. Results demonstrated that the hormonal response to an acute stressor could be detected in skin: the time from stress test to skin cortisol peak was an average of 46 days, whereas it was 55 days for corticosterone and 47 days for aldosterone. Results also showed that baseline hormonal concentrations were obtainable from skin samples collected during or immediately after the animals were subjected to the acute stressor. This study further develops and validates a non-invasive method for measuring cortisol and other hormones related to stress, health, and reproduction in the skin of cetaceans, potentially supporting investigations of acute and chronic stress, such as cetacean endocrine responses to distinct (e.g. naval sonar exposure) or prolonged stressors (e.g. shipping noise).
Non-coding RNA therapeutics for cardiac regeneration
Authors: Braga Luca , Ali Hashim , Secco Ilaria , Giacca Mauro 
Published: March 2020

AbstractA growing body of evidence indicates that cardiac regeneration after myocardial infarction can be achieved by stimulating the endogenous capacity of cardiomyocytes (CMs) to replicate. This process is controlled, both positively and negatively, by a large set of non-coding RNAs (ncRNAs). Some of the microRNAs (miRNAs) that can stimulate CM proliferation is expressed in embryonic stem cells and is required to maintain pluripotency (e.g. the miR-302∼367 cluster). Others also govern the proliferation of different cell types, including cancer cells (e.g. the miR-17∼92 cluster). Additional miRNAs were discovered through systematic screenings (e.g. miR-199a-3p and miR-590-3p). Several miRNAs instead suppress CM proliferation and are involved in the withdrawal of CMs from the cell cycle after birth (e.g. the let-7 and miR-15 families). Similar regulatory roles on CM proliferation are also exerted by a few long ncRNAs. This body of information has obvious therapeutic implications, as miRNAs with activator function or short antisense oligonucleotides against inhibitory miRNAs or lncRNAs can be administered to stimulate cardiac regeneration. Expression of miRNAs can be achieved by gene therapy using adeno-associated vectors, which transduce CMs with high efficiency. More effective and safer for therapeutic purposes, small nucleic acid therapeutics can be obtained as chemically modified, synthetic molecules, which can be administered through lipofection or inclusion in lipid or polymer nanoparticles for efficient cardiac delivery. The notion that it is possible to reprogramme CMs into a regenerative state and that this property can be enhanced by ncRNA therapeutics remains exciting, however extensive experimentation in large mammals and rigorous assessment of safety are required to advance towards clinical application.
Failure rates among metal brackets cured with two high-intensity LED light-curing lamps: anin vivostudy
Authors: Albertin Silvia Americo , Pinzan-Vercelino Célia Regina Maio , Flores-Mir Carlos , Gurgel Júlio de Araújo 
Published: March 2020

SummaryObjectiveThe objective of this split-mouth clinical trial was to compare bonding failure rates of metal brackets bonded with two different light-emitting diode (LED) light-curing lamps with different high-intensity power outputs.Materials and methodsForty patients were included for a total of 800 brackets that were randomly bonded (left and right sides in a 1:1 ratio) in maxillary and mandibular arches using two different LED devices. An LED of 3200 mW/cm2 and an LED of 5000 mW/cm2 were used in this split-mouth clinical trial. Bonding failures during the initial 6 months of orthodontic treatment were recorded as maxillary versus mandibular, anterior teeth versus posterior teeth, and left side versus right side.ResultsFive dropouts were recorded for discontinuing orthodontic treatment and 700 brackets were analysed in total. The bonding failure rates for 3200 and 5000 mW/cm2 LEDs were 6.0 and 7.4 per cent, respectively (P = 0.450), which were not statistically significantly different. There were no significant differences in bracket survival rates between the LEDs used (P = 0.866). The posterior teeth presented a higher index of bond failures (odds ratio, 3.14; 95% confidence interval, 1.68–5.87; P &lt; 0.001).LimitationsDirect comparison was only done between two high-intensity LED lights rather than against conventionally used halogen lights.ConclusionSimilar bonding failures were recorded using both LED devices (3200 and 5000 mW/cm2). Significantly more bonding failures occurred in premolar teeth than in anterior teeth.
Parental consumption of ultra-processed, high-fat products has no association with childhood overweight/obesity: an epidemiological study among 10–12-years-old children in Greece
Authors: Kanellopoulou Aikaterini , Antoniou Eleni , Notara Venetia , Antonogeorgos George , Rojas-Gil Andrea-Paola , Kornilaki Ekaterini , Kordoni Maria-Eleni , Velentza Anna , Mesimeri Magdalini , Lagiou Areti , Panagiotakos Demosthenes B 
Published: March 2020

AbstractBackgroundOverweight and obesity during childhood consist two of the most important public health issues in the 21st century. Consumption of high-fat processed food has been increased alarmingly.ObjectiveTo examine the association between parental ultra-processed, high-fat products’ consumption and childhood overweight/obesity.MethodsA cross-sectional survey, conducted among 422 children, aged 10–12 years, and their parents, during school years 2014–16. Parental and child data were collected through self-administered, anonymous and validated questionnaires. Among others, high-fat ultra-processed food consumption was also recorded. Children’s weight status was based on gender- and age-specific tables derived from the International Obesity Task Force body mass index (BMI) cut-offs.ResultsThe prevalence of obesity in the reference population was 2.9%, whereas the prevalence of overweight was 19.3%. A strong correlation was observed between children’s and their parents’ BMI status (P &lt; 0.001). Multi-adjusted data analysis revealed no association between parental intake of ultra-processed, high-fat products and children overweight/obesity. Similarly, when the data analysis accounted for family income and physical activity status of the children, the aforementioned results remained insignificant.ConclusionDespite the fact that parents’ specific dietary habits seem not to affect their children’s weight status, public health programs should consider parental nutrition education and mobilization as a preventive measure for childhood overweight/obesity.
Long-term combined administration of Bifidobacterium bifidum TMC3115 and Lactobacillus plantarum 45 alleviates spatial memory impairment and gut dysbiosis in APP/PS1 mice
Authors: Wang Feng , Xu Tong , Zhang Yujie , Zheng Tingting , He Yunling , He Fang , Jiang Yugang 
Published: March 2020

ABSTRACT
This study aimed to determine the effects of Bifidobacterium bifidum TMC3115, Lactobacillus plantarum 45 (LP45) and their combined use on cognitive performance and gut microbiota in APP/PS1 mice. The APP/PS1 mice were randomly divided into four groups: Alzheimer's disease (AD) model group, TMC3115 group [1 × 109 colony forming unit (CFU)], LP45 group (1 × 109 CFU) and a mixture group of TMC3115 (5 × 108 CFU) and LP45 (5 × 108 CFU). The wild-type littermates were chosen as normal control. The mice were sacrificed at the end of 22 weeks after behavioral evaluation. Collected cecum content was analyzed using 16S rRNA sequencing. Combined use of TMC3115 and LP45 significantly increased the times across the platform, time spent in the target quadrant compared with the AD, TMC3115 and LP45 groups in Morris water maze test. Microbiota analysis showed that combined TMC3115 and LP45 supplementation significantly increased observed species and beta diversity, and reversed gut dysbiosis by decreasing the abundance of Bacteroides and increasing the abundance of Acetatifactor and Millionella. These results indicate the long-term combined administration of TMC3115 and LP45 can improve spatial memory impairment in APP/PS1 mice and suggest that modifying the gut microbiome may provide potential benefits for AD patients.
Diabetes mellitus type 2 drives metabolic reprogramming to promote pancreatic cancer growth
Authors: Velazquez-Torres Guermarie , Fuentes-Mattei Enrique , Choi Hyun Ho , Yeung Sai-Ching J , Meng Xiangqi , Lee Mong-Hong 
Published: March 2020

AbstractBackgroundDiabetes mellitus type 2 (DM2) is a modifiable risk factor associated with pancreatic carcinogenesis and tumor progression on the basis of epidemiology studies, but the biological mechanisms are not completely understood. The purpose of this study is to demonstrate direct evidence for the mechanisms mediating these epidemiologic phenomena. Our hypothesis is that DM2 accelerates pancreatic cancer growth and that metformin treatment has a beneficial impact.MethodsTo determine the effect of glucose and insulin in pancreatic cancer proliferation, we used conditioned media to mimic DM2 conditions. Also, we studied the effect of anti-diabetic drugs, particularly metformin and rosiglitazone on pancreatic cancer growth. We established orthotopic/syngeneic (Leprdb/db) mouse cancer models to evaluate the effect of diabetes on pancreatic tumor growth and aggressiveness.ResultsOur results showed that diabetes promotes pancreatic tumor growth. Furthermore, enhanced tumor growth and aggressiveness (e.g. epithelial–mesenchymal transition) can be explained by functional transcriptomic and metabolomic changes in the mice with diabetes, namely via activation of the AKT/mTOR pathway. Metformin treatment suppressed the diabetes-induced AKT/mTOR pathway activation and tumor growth. The metabolic profile determined by mass spectrum showed important changes of metabolites in the pancreatic cancer derived from diabetic mice treated with metformin.ConclusionsDiabetes mellitus type 2 has critical effects that promote pancreatic cancer progression via transcriptomic and metabolomic changes. Our animal models provide strong evidence for the causal relationship between diabetes and accelerated pancreatic cancers. This study sheds a new insight into the effects of metformin and its potential as part of therapeutic interventions for pancreatic cancer in diabetic patients.
Analysis of pneumothorax recurrence risk factors in 843 patients who underwent videothoracoscopy for primary spontaneous pneumothorax: results of a multicentric study
Authors: Cattoni Maria , Rotolo Nicola , Mastromarino Maria Giovanna , Cardillo Giuseppe , Nosotti Mario , Mendogni Paolo , Rizzi Alessandro , Raveglia Federico , Siciliani Alessandra , Rendina Erino A , Cagini Lucio , Matricardi Alberto , Filosso Pier Luigi , Passone Erika , Margaritora Stefano , Vita Maria Letizia , Bertoglio Pietro , Viti Andrea , Imperatori Andrea 
Published: March 2020

Abstract

OBJECTIVES
Risk factors for pneumothorax recurrence after videothoracoscopy for primary spontaneous pneumothorax are still being debated. The goal of this study was to assess whether the pleurodesis technique and other variables are possibly associated with the postoperative ipsilateral recurrence of pneumothorax.

METHODS
We retrospectively collected data of 1178 consecutive ≤40-year-old patients who underwent videothoracoscopy for primary spontaneous pneumothorax in 9 centres between 2007 and 2017. We excluded patients with hybrid pleurodesis and/or incomplete follow-up, leaving for analysis 843 cases [80% men; median age (interquartile range) 22 (18–28) years]. Univariable and multivariable analyses were performed by logistic regression and tested by Cox regression model to assess factors related to ipsilateral pneumothorax recurrence including age, gender, body mass index, smoking habit, cannabis smoking, respiratory comorbidity, dystrophic severity score, surgical indication, videothoracoscopy port number and side, lung resection, pleurodesis technique and postoperative prolonged air leak (&gt;5 days).

RESULTS
Blebs/bullae resection was performed in 664 (79%) patients. Pleurodesis was achieved by partial pleurectomy in 228 (27%) cases; by pleural electrocauterization in 176 (21%); by pleural abrasion in 121 (14%); and by talc poudrage in 318 (38%). During a median follow-up period of 70.0 months (95% confidence interval 66.6–73.4), pneumothorax recurred in 79 patients (9.4%); among these, 29 underwent redo surgery; 34, chest drain/talc slurry; and 16, clinicoradiological observation. The only independent risk factor for recurrence was postoperative prolonged air leak (P &lt; 0.001) that was significantly related to blebs/bullae resection (P = 0.03).

CONCLUSIONS
In this multicentric series, postoperative ipsilateral pneumothorax recurrence was remarkable and independently related to prolonged postoperative air leak; besides the retrospective study setting, the pleurodesis method did not have an impact on recurrence. To prevent prolonged air leak, blebs/bullae treatment should be accurate and performed only if indicated.
Construct-Specific Loop-Mediated Isothermal Amplification: Rapid Detection of Genetically Modified Crops with Insect Resistance or Herbicide Tolerance
Authors: Singh Monika , Pal Deepa , Sood Payal , Randhawa Gurinderjit 
Published: March 2020

Abstract

Background
Insect resistant and herbicide tolerant genetically modified (GM) events have been approved in many countries. Screening methods could facilitate preliminary testing to check the GM status, which may target control elements, transgenes, and marker genes or construct regions. Among these, methods targeting the construct region, i.e., the junction between two genetic elements of a transgenic cassette are more specific.

Objective
Loop-mediated isothermal amplification (LAMP) assays targeting three construct regions were developed; between Cauliflower Mosaic Virus 35S promoter and cry1Ac gene (p35S-cry1Ac), cry2Ab2 gene and nos terminator (cry2Ab2-tnos), and cp4-epsps gene and nos terminator (cp4epsps-tnos).

Method
LAMP assays were performed by incubation at constant temperatures for selected targets. Positive amplification was detected as a change in color from orange to green on addition of SYBR® Green dye in visual LAMP and as real-time amplification curves in real-time LAMP.

Results
These assays showed acceptable specificity and sensitivity. Visual LAMP was found to be sensitive enough to detect as low as 0.005%, equivalent to two target copies. Real-time LAMP assays were able to detect as low as four copies of the target within 40 min, making them suitable for rapid on-site testing for GM organisms (GMO). Practical utility was also verified using spiked test samples.

Conclusions
These assays could be employed to address some of the biosafety or post-release monitoring issues, as well as to check for approved and unapproved GM events in a country.

Highlights
LAMP assays targeting three construct regions have been developed, enabling screening for approved or unapproved GMO.
The relationship between feed intake and liveweight in domestic animals
Authors: Lewis Ronald M , Emmans Gerry C 
Published: March 2020

Abstract
Feed intake changes as animals age and grow. A constraint of most functional forms used to describe this relationship is that intake is maximum only once an animal reaches its mature weight. Often such is not the case and maximum intake is achieved earlier. Our aim was to describe a form unburdened by such a constraint and to determine its utility to describe the relationship between feed intake and liveweight across multiple species. Twelve data sets representing seven domestic animal species (cattle, chicken, dog, pig, rat, sheep, and turkey) with a wide range of mature weights were used. Average daily ad libitum feed intakes and liveweights were available on either a weekly or fortnightly basis. Rates of intake were scaled to mature intake. Within each set, the quadratic regression of scaled intake on the degree of maturity in weight was fitted. This form provided a very good description of the relationship between these variables (R2 &gt; 0.86) and, for all but one case, a realistic prediction of mature intake. With one exception, intake reached its maximum value at a liveweight below its mature value. Furthermore, by appropriately scaling the relationship between intake and liveweight, the data could be described by a function with a single parameter with general relevance across species. By expressing the rate of intake as a function of its value at maturity, a quadratic form provides a robust and general description of the relationship between feed intake scaled to mature intake and degree of maturity in weight.
“Awake” Extracorporeal Membrane Oxygenation Combined With Continuous Renal Replacement Therapy For the Treatment of Severe Chemical Gas Inhalation Lung Injury
Authors: Li Yang , Cao ChunShui , Huang Liang , Xiong HuaWei , Mao HongTao , Yin Qin , Luo XiaoLong 
Published: March 2020

Abstract
Lung injury caused by chemical gas inhalation is a common clinically severe disease that very easily progresses to acute respiratory distress syndrome (ARDS). Traditional respiratory support consists mainly of mechanical ventilation, but the prognosis of this condition is still poor. “Awake” extracorporeal membrane oxygenation (ECMO) maintains oxygenation, improves ventilation, adequately allows the injured lungs to rest, and avoids complications associated with sedation, intubation, and mechanical ventilation. Continuous renal replacement therapy (CRRT) can provide better fluid management and reduce pulmonary edema. Herein, we describe the case of a patient with severe chemical gas inhalation lung injury who failed to respond to traditional mechanical ventilation and was subsequently treated with awake ECMO combined with CRRT.