bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of prognostic genes in uveal melanoma microenvironment
Authors: Luo Huan , Ma Chao 
Published: November 2020

Background
Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. Many previous studies have demonstrated that the infiltrating of immune and stromal cells in the tumor microenvironment contributes significantly to prognosis.

Methods
Dataset TCGA-UVM, download from TCGA portal, was taken as the training cohort, and GSE22138, obtained from GEO database, was set as the validation cohort. ESTIMATE algorithm was applied to find intersection differentially expressed genes (DEGs) among tumor microenvironment. Kaplan-Meier analysis and univariate Cox regression model were performed on intersection DEGs to initial screen for potential prognostic genes. Then these genes entered into the validation cohort for validation using the same methods as that in the training cohort. Moreover, we conducted correlation analyses between the genes obtained in the validation cohort and the status of chromosome 3, chromosome 8q, and tumor metastasis to get prognosis genes. At last, the immune infiltration analysis was performed between the prognostic genes and 6 main kinds of tumor-infiltrating immune cells (TICs) for understanding the role of the genes in the tumor microenvironment.

Results
959 intersection DEGs were found in the UM microenvironment. Kaplan-Meier and Cox analysis was then performed in the training and validation cohorts on these DEGs, and 52 genes were identified with potential prognostic value. After comparing the 52 genes to chromosome 3, chromosome 8q, and metastasis, we obtained 21 genes as the prognostic genes. The immune infiltration analysis showed that Neutrophil had the potential prognostic ability, and almost every prognostic gene we had identified was correlated with abundances of Neutrophil and CD8+ T Cell.

Conclusions
Identifying 21 prognosis genes (SERPINB9, EDNRB, RAPGEF3, HFE, RNF43, ZNF415, IL12RB2, MTUS1, NEDD9, ZNF667, AZGP1, WARS, GEM, RAB31, CALHM2, CA12, MYEOV, CELF2, SLCO5A1, ISM1, and PAPSS2) could accurately identify patients' prognosis and had close interactions with Neutrophil in the tumor environment, which may provide UM patients with personalized prognosis prediction and new treatment insights.
Malaria and curable sexually transmitted infections in pregnant women: A two-years observational study in rural Burkina Faso
Authors: Zango Serge Henri , Lingani Moussa , Valea Innocent , Samadoulougou Ouindpanga Sékou , Bihoun Biebo , Rouamba Toussaint , Derra Karim , Rouamba Eli , Donnen Phillipe , Dramaix Michele , Tinto Halidou , Robert Annie 
Published: November 2020

Background
Malaria and curable sexually transmitted infections (STI) are the most common curable infections known to have a severe impact on pregnancy outcomes in sub-Saharan Africa. This study aims to assess the marginal and joint prevalence of symptomatic cases of malaria and STI in pregnant women living in rural settings of Burkina Faso and their associated factors, after more than a decade of the introduction of intermittent preventive treatment (IPT-SP).

Methods
We carried out an observational study in two health districts in rural Burkina, namely Nanoro and Yako. Routine data were collected during antenatal and delivery visits for all women who delivered in the year 2016 and 2017. Logistic regression models were used to assess factors associated with infections.

Results
We collected data from 31639 pregnant women attending health facilities. Malaria, curable STI and their coinfections were diagnosed in 7747 (24.5%; 95%CI: 24.0–25.0%), 1269 (4.0%; 95%CI: 3.8–4.2%) and 388 (1.2%; 95%CI: 1.1–1.4%) women, respectively. In multivariate logistic regression, malaria occurrence was significantly higher in pregnant women < 20 years (Adjusted OR = 2.36; 95% CI: 2.07–2.69) than in women ≥30 years. The prevalence of curable STI was also significantly higher in students (Adjusted OR = 1.93; 95% CI: 1.26–2.95) and compensated workers (Adjusted OR = 1.52; 95% CI: 1.01–2.17) than in uncompensated workers. Women who received no IPT-SP had higher prevalence of malaria (Adjusted OR = 3.33; 95%CI: 3.00–3.70), curable STI (Adjusted OR = 1.96 95%CI: 1.60–2.39) and coinfections (Adjusted OR = 2.11; 95% CI: 1.50–2.95) compared to women who received SP.

Conclusion
Malaria and curable STI remain highly prevalent in rural settings of Burkina Faso, with young pregnant women and women who received no IPT-SP being the most affected. Prevention must be reinforced to improve maternal and infant health.
Cost-effectiveness of Interventions to Increase HPV Vaccine Uptake
Authors: Spencer Jennifer C. , Brewer Noel T. , Trogdon Justin G. , Weinberger Morris , Coyne-Beasley Tamera , Wheeler Stephanie B. 
Published: November 2020

OBJECTIVES:
We sought to prioritize interventions for increasing human papillomavirus (HPV) vaccination coverage based on cost-effectiveness from a US state perspective to inform decisions by policy makers.

METHODS:
We developed a dynamic simulation model of HPV transmission and progression scaled to a medium-sized US state (5 million individuals). We modeled outcomes over 50 years comparing no intervention to a one-year implementation of centralized reminder and recall for HPV vaccination, school-located HPV vaccination, or quality improvement (QI) visits to primary care clinics. We used probabilistic sensitivity analysis to assess a range of plausible outcomes associated with each intervention. Cost-effectiveness was evaluated relative to a conservative willingness-to-pay threshold; $50 000 per quality-adjusted life-year (QALY) .

RESULTS:
All interventions were cost-effective, relative to no intervention. QI visits had the lowest cost and cost per QALY gained ($1538 versus no intervention). Statewide implementation of centralized reminder and recall cost $28 289 per QALY gained versus QI visits. School-located vaccination had the highest cost but was cost-effective at $18 337 per QALY gained versus QI visits. Scaling to the US population, interventions could avert 3000 to 14 000 future HPV cancers. When varying intervention cost and impact over feasible ranges, interventions were typically preferred to no intervention, but cost-effectiveness varied between intervention strategies.

CONCLUSIONS:
Three interventions for increasing HPV vaccine coverage were cost-effective and offered substantial health benefits. Policy makers seeking to increase HPV vaccination should, at minimum, dedicate additional funding for QI visits, which are consistently effective at low cost and may additionally consider more resource-intensive interventions (reminder and recall or school-located vaccination).
Sexual experience among postmenopausal women in Yogyakarta: a qualitative study
Authors: Vidayanti Venny , Retnaningsih Listyana Natalia 
Published: November 2020

Background: Women in the postmenopausal period will experience a hormonal shift that makes them facing difficulties ranging from symptoms to complication. Vaginal dryness, hot flashes, decreased thinned vaginal mucosa may cause painful intercourse. This problem may adversely affect sexual activity among postmenopausal women such as a decrease in libido or sexual desire, inability to reach orgasm, pain during sexual intercourse, and fatigue. This study aims to explore the sexual experience among postmenopausal women in a rural area in Yogyakarta.Methods: This was a descriptive qualitative study. Twelve participants aged 50-60 years old who met the criteria, were purposively enrolled in this study. Individual face to face, semi-structured and in-depth interviews were performed for data collection. All conversations were audio-recorded and transcribed. We employed a conventional content analysis to derive coding categories from raw data.Results: Three main themes emerged from data analysis: (1)â€œnegative changes in sexual functionâ€ (decreased in frequency of sex, low libido, vaginal dryness, anorgasmia, and fatigue), (2)â€ intimacy with the coupleâ€ (couple communication and romance, lack of physical intimacy),Â (3)â€ coping with the symptoms in postmenopausal periodâ€ (acceptance of negative changes in sexual function, improve healthy diet and physical activity).Conclusion: Postmenopausal women experienced negative changes in sexual function. Most of them related to the lack of intimacy with the couple and having a positive coping strategy to improve their sexual relationship. Health education intervention strategy is one of the alternative approaches to enhance womenâ€™s attitude and coping in the postmenopausal period.
Correlation between Stress Scale and Severity of Acne Vulgaris
Authors: Regina Sutrisno Angie , Karmila Jusuf Nelva , Budi Putra Imam 
Published: November 2020

Background: Acne vulgaris (AV) is a chronic inflammatory disease of pilosebaceous follicles with polymorphic lesions, consist of blackheads, papules, pustules and nodules with varying degree of severity. Stress is one of the triggers for acne vulgaris.Aim: This study aimed to determine the correlation between stress scale with the severity of acne vulgaris. Â Methods: This study was an observational analytic study with a cross-sectional design involving 100 patients with acne vulgaris. The dermatological examination was performed in each patient to determine the severity of acne vulgaris with Acne Grading Indonesian Acne Expert Meeting 2015 based on Lehmann Criteria, followed by Holmes-Rahe stress scale questionnaire to evaluate stress scale. The collected data were analysed using the Spearman correlation test.Results: Of the total 100 samples examined, the majority of patients with acne vulgaris were 17-25 years old (71%) and most of them were female (68%). The majority of research subjects were classified with moderate acne vulgaris (45%), followed by mild (43%) and severe (12%). Low-stress scale (44%) is mostly found in this study, followed by a medium stress scale (29%) and high (27%). There is a positive correlation between stress scale and the severity of acne vulgaris with moderate strength (r = 0.456; p = 0.001).Conclusion: It can be concluded in this study that there is an association between stress scale and the severity of acne vulgaris.
Tie versus clipping typed of cystic duct and artery ligation in laparoscopic cholecystectomy
Authors: Ismaeil Deari Ahmad , Shkor Fazladdin N. , Salih Abdulwahid Mohammed , Ahmed Muhsin Muhammad , Said Ary Hama , Ali Halkawt Omar , Jalal Hardi Kareem 
Published: November 2020

Background: In laparoscopic cholecystectomy (LC) cystic duct and cystic artery (CD-CA) are usually secured by using Titanium clips. Because of some complications may occur in application of clips; like dislodgement and migration of the clips, so other different techniques have been used, like intra or extracorporeal ligation, using of absorbable clips, harmonic scalpel or LigaSure. Intra-corporeal ligation takes more time than applying a clip, and it needs well training. In the other hand clipping have a challenge in cases with wide cystic duct. The objective of the study is to compare the efficacy and safety of suture ligation versus clipping, in securing CD-CA in LC, and also time difference of operations in both methods.Methods: A Prospective study included 94 females, and 32 males underwent LC; in 51Â patients CD-CA were secured by intra-corporeal ligation separately, and in 75Â patients CD-CA were secured by Titanium clips. Postoperatively the patients followed up in the outpatient clinic at the end of the first and second postoperative weeks for any complication.Results: There were no significant differences in the operation times between the two groups (p-value: 0.08), and also no significant difference found between the operation times and the different presentations, history of upper abdominal surgery, present of umbilical hernias, and different BMI. There was no per-operative bile duct injury and no postoperative bile leak, intraperitoneal collection, or jaundice, in both groups.Conclusion: Suture ligation for CD-CA is safe and effective. Learning intra-corporeal knotting is important and plays an important role even in addition to the use of the clipping, and should be the recommended training in basic laparoscopic surgery.
Awake craniotomy procedure for near eloquent cortical area for brain tumor case series: initial experience and the anesthetic challenges
Authors: Arifin Muhamad Thohar , Setiawan Fariz Eka , Prihastomo Krisna T. , Bintoro Aris Catur , Harahap Sofyan , Sasongko Himawan , Bakhtiar Yuriz , Priambada Dody , Risdianto Ajid , Brotoarianto Happy Kurnia Brotoarianto Kurnia , Andar Erie BPS , Kusnarto Gunadi , Karlowee Vega , Muttaqin Zainal 
Published: November 2020

Introduction: The main objective of the surgical procedure is to achieve maximum resections with minimal functional neurological deficits for the patient with intrinsic tumors near the eloquent cortical area. The awake craniotomy procedure is currently the key option for achieving optimum safe resection. We report our two years of experience in establishing an awake craniotomy in the Kariadi General Hospital, evaluating the adoption of the technique and the outcome of the surgery.Methods: This is a retrospective study analyzed data from all patient's medical records, who have performed an awake craniotomy from January 2018 to January 2020 at Dr Kariadi General Hospital, Semarang, Indonesia. The specific anesthesia technique designated for this procedure was adopted.Â Sonography was introduced to determine the border of the tumor before and after surgery. Phase reversal using the cortical grid was used to recognize the central sulcus, motor and sensory cortex. Cortical stimulation using a monopolar stimulator was used to recognize the eloquent region surrounding the tumor. Enbloc resection was done with a fully conscious patient as well as with careful neurological testing during surgery.Result: The pre-operative Karnofsky Performance Status (KPS) mean 63, with remarkable improvement to 70. The length of surgery was varying from 120 to 420 minutes with mean was 270 min. Our procedure was done for tumors situated in the Broca area in 3 cases, motor gyrus in 7 cases and premotor gyrus in 3 cases. None of the patients needed intensive post-operative care. Pathological findings show glioma in 9 patients, metastases in 3 patients and tuberculoma in 1 patient.Conclusion: Based on our experience, Awake craniotomy is a technique designed to preserve the eloquent cortex and improving our knowledge of the functional structure of brainÂ centers. Together with the neurosurgeon, neurophysiologist, neuro anesthesia, and operating room (OR) nurse team, these advanced neurosurgery procedures can be performed without hesitation in developing countries.
Uji efektivitas ekstrak air daun ubi jalar ungu (Ipomoea batatas) terhadap profil lipid tikus putih jantan galur wistar yang diinduksi pakan dislipidemia
Authors: Heriwijaya I Pande Putu Deny , Jawi I Made , Satriyasa Bagus Komang 
Published: November 2020

Background: The conventional drugs for dyslipidemia theraphy needed to be concerned in the usage because of the side effect of the drugs. The Anthocyanin was one of the flavonoid compound that can be used as an alternative theraphy for dyslipidemia. The leaf of the purple sweet potato have been proved containing anthocyanin.Aim: The study aims to know the effectivity of the sweet potato leaf on lipid profile of male wistar rats induced by dyslipidemia feed extract.Method: The study design was pre and post-test control group design. Thirty white male rats were divided into five groups. All of the groups were given high-cholesterol diet for three months. Negative control groups of rats were given only high-cholesterol diet alone, positive control groups were given dyslipidemia drugs and the treatment groups were given sweet potato leaf water extract with the doses of each group were 3cc, 6cc and 9cc. Before and after treatment, lipid profile levels were measured.Results: There was a decrease in total cholesterol, triglyseride and LDL in treatmen group with 6cc and 9cc dose significantly (p<0,05) compared with the negative control group.Conclusion: The sweet potato leaf extract can improve the lipid profile of the dyslipidemia rats.Â Latar Belakang: Obat konvensional yang biasa dipakai untuk terapi dari dislipidemia masih harus diperhatikan dalam pemakaiannya karena masih adanya efek samping yang tidak diinginkan. Senyawa antosianin merupakan salah satu senyawa flavonoid yang dapat berpotensi sebagai terapi alternatif untuk dislipidemia. Pada daun ubi jalar ungu terbukti terdapat senyawa antosianin yang dapat digunakan sebagi terapi alternatif dislipidemia. Tujuan: untuk mengetahui efektivitas dari ekstrak daun ubi jalar ungu (Ipomoea batatas) terhadap profil lipid tikus putih jantan galur wistar yang diinduksi pakan dislipidemia. Metode: Rancangan penelitian adalah pre and post-test control group design. Penelitian dilakukan pada 30 ekor tikus putih jantan yang dibagi menjadi lima kelompok. Kelima kelompok tikus diberi makanan tinggi kolesterol selama tiga bulan. Pada kelompok tikus kontrol negatif hanya diberi pakan kolesterol, kontrol positif diberikan juga obat dislipidemia dan tiga kelompok perlakuan diberikan ekstrak air daun ubi ungu dengan dosis masing-masing kelompok 3cc, 6cc dan 9cc. Sebelum dan setelah mendapat perlakuan dilakukan pengukuran profil lipid. Hasil: Terdapat penurunan pada kolesterol total, trigliserida dan LDL pada kelompok perlakuan dengan dosis 6cc dan 9cc secara signifikan (p<0,05) dibandingkan dengan kontrol negatif. Kesimpulan: Pemberian ekstrak air daun ubi jalar ungu dapat memperbaiki profil lipid tikus dislipidemia.
Stefan Wyszyński’s Personalistic Vision of Upbringing
Authors: Gruca-Miąsik Urszula , Parzyszek Magdalena 
Published: November 2020

The personalistic concept of man, especially the one based on the philosophy of St. Thomas Aquinas, underscores the full autonomy of the human person, his freedom, the gradual discovery of the truth and good, and choices in accord with this cognition. The key issue in Stefan Wyszyński’s personalism is the integral vision of the human person. This personalism is a system of upbringing that respects the nature and value of man who ‘humanizes’ the world and expresses himself while constantly evolving. He gives a personal, social and dynamic character to the community in which he functions. He has a right to be brought up in keeping with his nature and ultimate destiny.
Estimation of depth and degree of mill roll surface hardening during grit texturing
Authors: Zvyagina E. Yu. , Ogarkov N. N. , Polyakova M. A. , Sukhova M. D. 
Published: November 2020

The paper presents shot blasting (SB) as the most common method of arrangement of temper mills rolls surface microgeometry, providing required roughness of the cold-rolled strip. The surface riffing studies were carried out at the Willibrator industrial unit; metallographic studies were performed using MEIJI 2700 optical microscope and JSM-6490LV scanning electron microscope. Refinement of the surface layer structure during interaction of the roll material with the grit was revealed. In the work the lower bound method, one of the methods of plasticity theory, is used for theoretical analysis of the SB process. To make computations easier, the roll microcave is approximated to spherical shape. Quantitative estimates of deformation degree and the depth of hardened layer were determined by speed of the grit and hardness of the roll surface. The depth of hardened layer is obtained by the size of used grit, and to a lesser extent, by the speed of grit impact on the roll surface and textured surface hardness. It has been searched out in literature that increase in hardness by one unit leads in average to 3 % increase in resistance. Thus, SB application allows reduction of time of rolls rehandling of temper mills by 6.0 – 10.5 %, depending on application modes of rolls riffing with grit. Analysis of microstructure have shown that increase in hardness of the surface layer is a consequence of refinement of its structure in process of interaction with the grit. It has been revealed that increase in hardness of the surface layer leads to an increase in its wear resistance and fatigue strength. The authors of the work have found that at speed of 60 m/s the hardness increases by 3.5 units.
Predictive Pharmacological Activity of Galangal Rhizome (Alpinia galanga (L.) Willd.) Through in Silico Analysis as an Effort to Accelerate The Research of Indonesian Medicinal Plants
Authors: Jamil Ahmad Shobrun , Ahmad Mujahidin 
Published: November 2020

Indonesia has high biodiversity, especially plant species. There are many benefits that can be Obtained from various plants that grow in Indonesia, one of which is as a health supplement or medicinal raw material. Fast researches are important in the use of these plants so that bio-based products can be widely accepted. One of the important fast methods in analysing the benefits of plant chemical compounds is the in-silico prediction utilizing metadata spread over various pages providing scientific data about plants, their chemical compound content and biological activity. This study was focused on predictively observing the biological activity of the compounds in the rhizome of Alpinia galanga. The research method is by analysing metadata from various sources. Data on the content of chemical compounds can be accessed through the page https://phytochem.nal.usda.gov/, classification of metabolite compounds contained in plants using http://classyfire.wishartlab.com/, prediction of absorption, distribution, metabolism and excretion (ADME) uses http://www.swissadme.ch/, to determine the relationship between plant compounds and body proteins, http://www.swisstargetprediction.ch/ and prediction of cellular mechanisms seen through https://string-db.org. Based on in silico analysis by utilizing some of the above software, it can be seen that the rhizome of the Alpinia galangal plant has 80 active compounds, 47 have high bioavailability and 9 compounds with tight cell proteins. Based on in silico exploration, it is also known that A. galangal has potential as an antioxidant, antimicrobial, anti-cancer and various other pharmacological activities
FTIR and Chemometrics Application on Determination of Total Flavonoid Content of Pasak Bumi Root Extract (Eurycoma longifolia Jack.)
Authors: Triyasmono Liling , Ulfah Ana , Rizki Muhammad Ikhwan , Anwar Khoerul , Wianto Totok , Santoso Heri Budi 
Published: November 2020

The combination of FTIR and chemometrics is an alternative method on determination of total flavonoid content of pasak bumi (Eurycoma longifolia Jack.) root extract. This study aims to determine the method of FTIR and chemometrics can be used for determination of total flavonoid content and determine the total flavonoid content of E. longifolia root extract using FTIR and chemometrics. The samples from three growing area were determined their flavonoid content by colorimetric method and measured their absorbance by FTIR spectrophotometer. The analysis was done by PCA chemometrics to grouping IR spectra based on growing area and PLSR to determine prediction model of total flavonoid content of E. longifolia root extract. The best grouping and prediction model is shown by IR spectra in the range of wavenumbers 1800-1540 cm-1 with total variation is 99% and prediction model with equation y = 0.995x + 0.002 (R2 calibration = 0.995; R2 validation = 0.970; RMSEC = 0.008; RMSECV = 0.021). The total flavonoid content of E. longifolia root extract (% b/b ± SD) from Mandiangin, Condong, and Sabuai is 0.225 ± 0.009; 0.437 ± 0.007; and 0.466 ± 0.016 (R2 = 0.995 and RMSEP = 0.008). Based on this, the combination of FTIR and chemometrics can be used to predict the total flavonoid content of unknown E. longifolia root extract