bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osthole Inhibits Osteoclast Formation and Enhances Bone Mass of Bone Marrow Mesenchymal Stem cells by Activating β-catenin-OPG Signaling Pathway
Authors: Jin Zhen-Xiong , Liao Xin-Yuan , Da Wei-Wei , Zhao Yong-Jian , Li Xiao-Feng , Tang Dezhi 
Published: December 2020

Abstract
Summary Osthole has potential therapeutic applications due to its antiosteoporotic. Our study suggested that osthole attenuates osteoclast formation by stimulating the activation of β-catenin-OPG signaling and could be a potential agent to inhibit bone resorption. Introduction Osthole has potential therapeutic applications due to its antiosteoporotic. we performed study to test if OPG is the target gene of osthole-attenuated osteoclastogenesis. Methods In vivo, using 12-month-old male mice to evaluate the effect of osthole on bone mass. In vitro, Bone marrow stem cells (BMSCs) were isolated, extracted from 3-month-old C57BL/6J mice, 3-month-old β-cateninfx/fx mice, or 3-month-old OPG−/− mice and its littermates of OPG+/+ mice. Results we found that osthole significantly increased the gene and protein levels of OPG expression in primary BMSCs dose-dependently. The deletion of the OPG gene did not affect β-catenin expression and the deletion of the β-catenin gene inhibited OPG expression in BMSCs, which indicated that osthole stimulated the expression of OPG through activation of β-catenin signaling. Conclusion Osthole attenuates osteoclast formation by stimulating the activation of β-catenin-OPG signaling and could be a potential agent to inhibit bone resorption.
Intelligent Long-Term Performance Analysis in Power Electronics Systems
Authors: Peyghami Saeed , Dragicevic Tomislav , Blaabjerg Frede 
Published: December 2020

Abstract
This paper proposes a long-term performance indicator for power electronic converters based on their reliability. The converter reliability is represented by the proposed constant lifetime curves, which have been developed using Artificial Neural Network (ANN) under different operating conditions. Unlike the state-of-the-art theoretical reliability modeling approaches, which employ detailed electro-thermal characteristics and lifetime models of converter components, the proposed method provides a nonparametric surrogate model of the converter based on limited non-linear data from theoretical reliability analysis. The proposed approach can quickly predict the converter lifetime under given operating conditions without a further need for extended, time-consuming electro-thermal analysis. Moreover, the proposed lifetime curves can present the long-term performance of converters facilitating optimal system-level design for reliability, reliable operation and maintenance planning in power electronic systems. Numerical case studies evaluate the effectiveness of the proposed reliability modeling approach.
Public Support for Health Coverage for Migrant Populations in The COVID-19 Pandemic: The Roles of Infection Externalities and Impressions of Migrants' Home Place
Authors: HUANG Genghua , Peng Zhaiwen 
Published: December 2020

Abstract
Background: International population mobility is a significant challenge for the management of the coronavirus (COVID-19) pandemic. The suspension of flights and other traffic has been adopted to avoid more cases of imported infection by many countries, but these measures mean that most migrant populations, including immigrants, migrant and seasonal workers, international students, and others, have to stay in their host places. Therefore, equitable access to health services in the host places is crucial for them. Immigrant-receiving areas may encounter a dilemma when considering whether to extend health coverage of COVID-19 testing and treatment to immigrants. In this context, it is vital to understand public attitudes towards this issue since they explain and validate the policy responses. Methods: The data used in this study came from an survey conducted in March 2020. The survey targeted adults aged 18 and older who were local residents of nine cities in Guangdong province, China. The sample size was 1,040, and STATA 15.0 was used in the statistical analysis.Results: The results show that individuals with higher health security ratings, lower demand for health services, and greater concern about the risk of infectious diseases are more supportive of extending health services to immigrants. In addition, individuals who rate immigrants’ home place as more immigrant-friendly are also more supportive of it.Conclusions: Public support for health coverage for immigrants in the COVID-19 Pandemic is influenced by infection externalities and impression of immigrants’ home countries. This study attempts to contribute to the body of literature related to welfare attitudes and immigration. It also outlines a series of crucial implications for the global task of managing COVID-19.
Prevalence of Anxiety and Depression Among Cancer Patients During the COVID-19 Pandemic: a Systematic Review and Meta-analysis
Authors: Yu Hongping , Zhou Zewen , Mo Qiuyan , Zhou Xianguo , Liu Yingchun , Feng Shixiong 
Published: December 2020

Abstract
Background: The COVID-19 pandemic has significantly affected patients’ access to healthcare. Patients with malignant tumors have experienced treatment interruptions and difficulties in seeking medical attention, and these have affected their mental health. The objective of this review is to evaluate the current evidence on the prevalence of depression and anxiety in cancer patients during the COVID-19 pandemic.Methods: We searched the EMBASE, PubMed, WHO COVID-19 database, Cochrane Library, Web of Science, CBM, CNKI, Wanfangdata, CQVIP, and CBM electronic databases for relevant studies published from the first available day to June 30, 2020. Two researchers independently screened the literature based on predetermined criteria and assessed the risk of bias in each study. Random-effects meta-analyses was used to estimate the pooled prevalence of anxiety and depression among patients with malignant tumors. The review protocol has been registered in PROSPERO (CRD42020196421).Results: Nine eligible studies on 2,335 patients with malignant tumors were included, all of which were cross-sectional studies. The pooled prevalence of anxiety was 52.94% (95% CI: 36.98%-68.91%, I2=98.6%) in 9 studies, and the pooled prevalence of depression was 43.25% (95% CI: 25.85%-60.85%, I2=98.8%) in 6 studies.Conclusions: Evidence shows that a considerable proportion of patients with malignant tumors suffered from emotional distress during the COVID-19 pandemic. It is important to solve the problem of interruption or delay of treatment and strengthen psychological interventions for patients with malignant tumors during the pandemic.
Analysis of Merged Whole Blood Transcriptomic Datasets to Identify Circulating Molecular Biomarkers of Feed Efficiency in Growing Pigs
Authors: Messad Farouk , Louveau Isabelle , Renaudeau David , Gilbert Hélène , Gondret Florence 
Published: December 2020

Abstract
Background: Improving feed efficiency (FE) is an important breeding goal due to its economic and environmental significance for farm animal production. The phenotypic value is obtained by measuring individual feed consumption and average daily gain during a test period, which is costly and time-consuming. The identification of reliable predictors of FE may be a relevant strategy to reduce phenotyping efforts. Results: Gene expression data in the whole blood from three originally separated experiments were combined and analyzed by machine learning algorithms to propose predictive molecular biomarkers of FE traits in growing pigs. The original datasets included pure Large White pigs (females and barrows) of two lines divergently selected for residual feed intake (RFI), a measure of net FE, and in which individual feed conversion ratio (FCR) and blood microarray data were available. Merging the three datasets allowed considering FCR values (Mean = 2.85; Min = 1.92; Max = 5.00) for a total of n = 148 pigs with a large range of body weight (15 kg to 115 kg) and different test period duration (2 weeks to 9 weeks). Random forest (RF) and gradient tree boosting (GTB) were applied on the whole blood transcripts (26,322 annotated molecular probes) to identify the most important variables for binary classification on RFI groups and for a quantitative prediction of FCR, respectively. Samples have been partitioned between learning (n = 74) and validation sets (n = 74). Iterative steps in variable selection led to identify about three hundreds (328 to 391) molecular probes as important predictors for RFI or FCR and participating to various biological pathways. With the GTB algorithm, simpler models combining 34 expressed unique genes to classify pigs on RFI (100% of success), and 25 expressed unique genes to predict FCR values (R² = 0.80, RMSE = 8%) were proposed. Accuracy performance of RF models were slightly lower in classification and markedly lower in regression.Conclusion: From small subsets of genes expressed in the whole blood, it is possible to predict feed efficiency traits in growing pigs. This offers good perspectives for microchip design in selection or precision farming applications.
Genome-Wide&nbsp;Identification of Cyclin Dependent Kinase (CDK) Family Genes Influencing Adipocyte Differentiation in&nbsp;Cattle
Authors: Pan Cuili , Lei Zhaoxiong , Wang Shuzhe , Wang Xingping , Wei Dawei , Cai Xiaoyan , Luoreng Zhuoma , Wang Lei , Ma Yun 
Published: December 2020

Abstract
Background: The cyclin dependent kinases (CDKs) are protein kinases regulating important cellular processes such as cell cycle and transcription. A variety of studies have shown that many CDK genes also played a critical role during adipogenic differentiation. However, there is a lack of systematic research on the CDK gene family regulating bovine adipocyte differentiation.Therefore, this study aimed to characterize CDK family genes in bovine and study the expression pattern during adipocyte differentiation.Results: We performed a genome-wide analysis and identified 25, 25, 22, 21, 22, 24, 22 and 24 CDK genes in Bos taurus, Bos indicus, Hybrid-Bos taurus, Hybrid bos indicus, Bos grunniens, Bos mutus, Bison and Bubalus bubalis, respectively. All the CDK genes classified into 8 subfamilies through phylogenetic analysis. Chromosome localization displayed 25 bovine CDK genes distributed on 16 chromosomes. Collinearity analysis revealed that CDK family genes of Bos taurus were extensively homologous with Bos indicus, Hybrid-Bos taurus, Hybrid bos indicus, Bos grunniens and Bubalus bubalis. Tanscriptome analysis showed that several of the CDK family genes had relatively high expression levels in preadipocytes compared with differentiated adipocytes, which is generally similar to qPCR, indicating that it could have a significant function in the growth of the emerging lipid droplets.Conclusion: We performed a comprehensive analysis for the CDK family genes including identification, phylogenetic classification, structural characterization, chromosomal distribution, collinearity analysis and expression profile analysis by tanscriptome sequencing and qPCR. The results provide a basis for further study to determine the roles of CDK family genes in regulating adipocyte differentiation, which is beneficial for beef quality improvement.
Comprehensive Analysis of Differentially Expressed CircRNAs and CeRNA Regulatory Network&nbsp;in Porcine Skeletal Muscle
Authors: Li Meng , Zhang Na , Zhang Wanfeng , Hei Wei , Cai Chunbo , Yang Yang , Lu Chang , Gao Pengfei , Guo Xiaohong , Cao Guoqing , Li Bugao 
Published: December 2020

Abstract
Background: Circular RNA (circRNA), a novel class of non-coding RNA, has a closed-loop structure with important functions in skeletal muscle growth. The purpose of this study was to investigate the role of differentially expressed circRNAs (DEcircRNAs), as well as the DEcircRNA-miRNA-mRNA regulatory network, at different stages of porcine skeletal muscle development. Here, we present a panoramic view of circRNA expression in porcine skeletal muscle from Large White and Mashen pigs at 1, 90, and 180 days of age. Results: We identified a total of 5,819 circRNAs. DEcircRNA analysis at different stages showed 327 DEcircRNAs present in both breeds. DEcircRNA host genes were concentrated predominately in TGF-β, MAPK, FoxO, and other signaling pathways related to skeletal muscle growth and fat deposition. Further prediction showed that 128 DEcircRNAs could bind to 253 miRNAs, while miRNAs could target 945 mRNAs. The constructed ceRNA network plays a vital role in skeletal muscle growth and development, and fat deposition. Circ_0015885/miR-23b/SESN3 in the ceRNA network attracted our attention. miR-23b and SESN3 were found to participate in skeletal muscle growth regulation, also playing an important role in fat deposition. Using convergent and divergent primer amplification, RNase R digestion, and qRT-PCR, circ_0015885, an exonic circRNA derived from Homer Scaffold Protein 1 (HOMER1), was confirmed to be differentially expressed during skeletal muscle growth. In summary, circ_0015885 may further regulate SESN3 expression by interacting with miR-23b to function in skeletal muscle. Conclusions: This study not only enriched the circRNA library in pigs, but also laid a solid foundation for the screening of key circRNAs during skeletal muscle growth and intramural fat deposition. In addition, circ_0015885/miR-23b/SESN3, a new network regulating skeletal muscle growth and fat deposition, was identified as important for increasing the growth rate of pigs and improving meat quality.
Decreased Clock Gene Bmal1 Mediates Epileptogenesis via PCDH19 in Temporal Lobe Epilepsy
Authors: Wu Hao , Liu Yong , Liu Lishuo , Meng Qiang , Du Changwang , Li Kuo , Dong Shan , Zhang Yong , Li Huanfa , Zhang Hua 
Published: December 2020

Abstract
Clock genes not only regulate the circadian rhythm of physiological activities but also participate in the pathogenesis of many diseases. Previous studies have found the abnormal expression of clock genes in epilepsy. However, as the core clock gene, the molecular mechanism of Bmal1 in the epileptogenesis and seizures of temporal lobe epilepsy (TLE) is still unclear. To define the function of Bmal1, we firstly investigated the levels of Bmal1 and other clock proteins in the hippocampus in epilepsy. In the latency and chronic phases, the levels of Bmal1 were decreased compared with the control group. Knockout of Bmal1 in hippocampal dentate gyrus (DG) neurons of Bmal1flox/flox mice by Synapsin 1 (Syn1) promoter AAV (adeno-associated virus) lowered the threshold of seizures induced by pilocarpine administration. High throughput sequencing analysis showed that PCDH19 (protocadherin 19), a gene associated with epilepsy, was regulated by Bmal1. And the expression of PCDH19 was also decreased in the hippocampus of epileptic mice. Furthermore, the levels of Bmal1 and PCDH19 were higher in the patients with no hippocampal sclerosis (no HS), compared to HS International League Against Epilepsy (ILAE) type I and III. Altogether, these data suggest that decreased expression of clock gene Bmal1 may participate in the epileptogenesis and seizures via PCDH19 in TLE.
Side Effects of Azathioprine in Patients with Ocular Behçet’s Syndrome
Authors: Masoumi Maryam , Davatchi Fereydoun , Shahram Farhad , Akhlaghi Maassoumeh , Faezi Seyedeh Tahereh , Kavosi Hoda , Shadmanfar Soraya , Alikhani Majid , Moradi Soroush , Balasi Javad 
Published: December 2020

Abstract
BackgroundBehçet’s syndrome (BS) is a multifactorial, polygenic, autoinflammatory vasculitis characterized by recurrent oral and genital ulcers, uveitis, skin lesions, and arthritis. Azathioprine has been well established as an effective therapy among other immunosuppressive drugs; however, concerns remain about its safety. This study was conducted to determine the types and prevalence of adverse events related with azathioprine in patients with ocular BS.MethodsWe carried out a cross-sectional study of 165 patients with a confirmed diagnosis of BS who had ocular involvement. Data were collected retrospectively on disease-related characteristics and events including severity, recurrence, relapse, recovery and flare-up, as well as on azathioprine dosage and the duration of use.ResultsOf 165 enrolled patients, 103 (62.43%) were male and 62 (37.57%) were female. Adverse events of treatment with azathioprine occurred in 11 patients (6.67%). These side effects included elevation of liver enzymes in 4 patients (2.43%), hypersensitivity reaction in 2 patients (1.21%), leukopenia in 2 patients (1.21%), nausea in 1 patient (0.61%), skin tumor in 1 patient (0.61%), and concomitant pancreatitis and hepatitis in 1 patient (0.61%). Six of these patients discontinued treatment, and 5 of them tapered the dose. In addition, we observed severe leukopenia in 2 patients.ConclusionAdministration of azathioprine with due attention to the signs, symptoms, and severity of the disease, results in a reduced rate of adverse events and resolution of the ocular involvement in patients with BS.
Metabolic Engineering of Escherichia Coli for Ectoine Production With the Fermentation Strategy of Supplementing Amino Donor
Authors: Zhang Hao , Cao Yingying , Dong Yingsheng , Zhao Ming , Ma Yanqin , Luo Zhengshan , Li Sha , Xu Hong 
Published: December 2020

Abstract
Background: Ectoine, a compatible solute, has broad application prospects in food biotechnology, agriculture, medicine, and cosmetics because of its protective action on biological compounds. Industrially, ectoine is produced by halophilic bacteria in a complex process. Recently, various works focus on improving ectoine production by using engineered strains, but there are still problems of low yield and low ectoine production efficiency.Results: To overcome the drawback, a final metabolic engineered strain E. coli ET08 was constructed by eliminating lysine synthesis branch and by-product metabolic pathways, and ectoine production reached 10.2 g/L through culture medium optimization. Compared with nitrate, addition of ammonium salt contributed more to the ectoine synthesis. Furthermore, the ammonium sulphate boosted more ectoine titers than ammonium chloride and sodium glutamate. The analysis of transcriptional levels revealed that the ammonium sulfate enhanced ectoine biosynthesis by enhancing metabolic flux toward ectoine biosynthesis and providing affluent synthetic precursors. Ultimately, the ectoine production and yield of the E. coli ET08 reached 36.5 g/L and 0.3 g/g glucose with supplementing amino donor in a 7.5 L bioreactor.Conclusions: a novel potential metabolic engineered Escherichia coli for ectoine production was constructed. optimizing amino donor and analyzing the transcription levels conclude that ammonium sulfate, as the optimal amino donor, has a positive effect on ectoine synthesis. It is the first report about the effect of exogenous amino donor on ectoine fermentation by metabolic engineered strain. The maximum ectoine production and yield from glucose synthesized by E. coli were obtained by two-stage feeding fermentation with supplementing amino donor. It provides a novel strategy for the synthesis of ectoine by engineered strain in industry. This research provides the basis for an effective process for ectoine production, together with the further applications of ectoine in food and cosmetics, and could also be used to produce other high value amino acid derivative.
Correlation of Early Total Leucocyte Count and Red Cell Distribution Width With Outcome in Trauma
Authors: Madineni Bhavana Chowdary , N Sreeramulu P 
Published: December 2020

Abstract
Background: Trauma is the leading cause of death in young adults. Risk stratification in patients with trauma remains challenging. There is a need for a readily available prognostic method to categorise these patients.Aims & Objectives: We aim to measure and correlate red cell distribution width and total leucocyte count in patients presenting with trauma within 24 hours of injury with the outcome.Methods: We included 52 trauma patients who were admitted in RLJH from November 2019 to April 2020, who did not require emergency surgery and analysed prospectively. Complete blood picture within 24 hours of injury was analysed and outcome measured in terms of length of hospital stay. Results: There was a statistically significant correlation found between TLC and Length of hospital stay (p<0.001). The correlation between RDW and Length of hospital stay was not statistically significant. The analysis showed that increased hospital stay was associated with high TLC and RDW% done within 24 hours of injury. Conclusion: TLC and RDW can be used as readily available and simple markers in early prediction of higher morbidity in patients presenting with trauma.
Epidemiological Investigation on Symptoms and Influencing Factors of Noise-Induced Deafness in Officers and Soldiers After Live Firing Training
Authors: Shi Lei , Li Dong-Yan , Ma Li-Sha , Qi Guo-Wei , Zhao Hui , Yu Ning , Yang Shi-Ming 
Published: December 2020

Abstract
Objective To study the symptom and influencing factors of acute acoustic injury in soldiers after shooting training. Methods A questionnaire survey was conducted among 571 soldiers who participated in live firing training. The necessary information of soldiers, the history of noise exposure before enlistment, and acute acoustic injury symptoms after shooting practice were collected and analyzed statistically. Result The soldiers seldom contact with impulse noise before enlistment. Another environmental noise intensity is medium, and contact time is short; after enlistment, most of them have received shooting training. The most common symptom of acute acoustic injury is tinnitus. The low-frequency tinnitus is more common, and the degree of discomfort is low; A few soldiers wear protective equipment. Statistics found that military age, whether to wear protective equipment, blood type, is related to acute acoustic injury. Conclusion Acute acoustic injury is more likely to occur after exposure to impulse sound in shooting training, and tinnitus is the most common symptom. With the increase of military age, it is more likely to have acoustic injury symptoms. Wearing personal protective equipment can effectively reduce acoustic damage and the susceptibility to the noise of type A blood is low.