bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between physical function and onset of coronary heart disease in a cohort of community-dwelling older populations: The Septuagenarians, Octogenarians, Nonagenarians Investigation with Centenarians study
Authors: Klinpudtan Nonglak , Kabayama Mai , Godai Kayo , Gondo Yasuyuki , Masui Yukie , Akagi Yuya , Srithum Werayuth , Kiyoshige Eri , Sugimoto Ken , Akasaka Hiroshi , Takami Yoichi , Takeya Yasushi , Yamamoto Koichi , Ikebe Kazunori , Yasumoto Saori , Ogawa Madoka , Inagaki Hiroki , Ishizaki Tatsuro , Arai Yasumichi , Rakugi Hiromi , Kamide Kei 
Published: August 2020

Abstract
Objective: Physical function is a strong predictor of the adverse outcomes in older populations. We prospectively examined the association of walking speed and handgrip strength with CHD in the community-dwelling older populations. The study cohort in Japan included 1272 older people free from heart disease at the baseline. Physical function was identified based on walking speed and handgrip strength assessment at the survey site. Any new case of CHD was obtained based on a self-reported doctor's diagnosis. Cox-proportion hazard models were adjusted for covariate factors to examine the CHD risk. Results: During the 7-year follow-up, 45 new cases of CHD were documented. Slow walking speed was strongly associated with CHD risk after adjusting for all confounding factors in the total participants and women (hazard ratio (HR)= 2.53, 95%confidence interval (CI), 1.20-5.33, p=0.015, and HR= 4.78, 95% CI,1.07-21.35, p=0.040, respectively), but not in men. Weak grip strength was associated with CHD after age-adjustment (HR= 2.45, 95%CI, 1.03-5.81, p=0.043) only in men. However, after additional multivariate adjustment, the associations were weaker. The results lead to the conclusion that, after multivariate adjustment for coronary heart disease, walking speed is a strong surrogate predictor of the CHD risk among older people.
SHED-Derived Exosomes Regulate Microglial Polarization in the Treatment of Traumatic Brain Injury in Rats via miR-330-5p
Authors: Li Ye , Wang Xinxin , Cao Xiaoyu , Li Na , Meng Sun , Li Ang 
Published: August 2020

Abstract
Background: Traumatic brain injury (TBI) causes structural damage and impairs motor and cognitive function of the brain. Our previous study suggested that exosomes (EXs) secreted by stem cells from human exfoliated deciduous teeth (SHED) extenuated motor damage in TBI rats by regulating microglia. The molecular mechanism of SHED-EXs was investigated in the present study. Methods: The miRNA array was performed to determine the differential miRNA expression in SHED-EXs treating microglia. The key miRNA was selected. Flow cytometry, immunofluorescence, enzyme linked immunosorbent assay (ELISA) and Griess assay were performed to detect the function of key miRNA. Real-time PCR, Western blotting and dual luciferase reporter assay were used to confirm the relationship between key miRNA and the target gene. Chromatin immunoprecipitation (ChIP) was performed to determine the downstream pathway of EXs-miRNA. Traumatic brain injury rat model was established and local injection of EXs-miRNA was performed to evaluate the effect.Results: SHED-EXs delivery of miR-330-5p was the key in the regulation of microglia polarization by inhibiting M1 polarization and promoting M2 polarization. Mechanistically, miR-330-5p had an inhibitory effect on Ehmt2, and miR-330-5p/Ehmt2 promoted the transcription of CXCL14 through H3K9me2. In vivo data showed that SHED-EXs/miR-330-5p reduced neuro-inflammation and repaired neurological function of TBI rats. Conclusions: SHED-EXs/miR-330-5p improved the motor function of rats after TBI by inhibiting M1 polarization and promoting M2 polarization of microglia through Ehmt2/H3K9me2/CXCL14 pathway.
Inferences From Dysregulated Long Non-Coding RNA-Mediated Competing Endogenous RNAs in Various Chemotherapy Drugs and Evaluation of Drug Response in Breast Cancer
Authors: Zhang Ziwen , Li Dongbo , Zhang Han , Qin Qi , Zhang Qingyuan 
Published: August 2020

Abstract
Backgroud: Differences in individual drug response, especially drug resistance, present an obstacle to the treatment of breast cancer (BRCA). Thus, the ability to predict drug response would contribute to developing novel treatment strategies. Accumulating evidence have suggested that tumor molecular profiles and drug response data provide opportunities and challenges for the discovery of new molecular characteristics and mechanisms of drug response in BRCA. Methods: In the present study, an integrated pipeline was developed to explore drug response-related long non-coding RNA (lncRNA)-mediated competing endogenous RNAs (ceRNAs) motifs in BRCA. Results: Drug response-specific ceRNAs indicated that lncRNAs play an essential role in various drug treatments for BRCA. Several key drug-resistant and -sensitive dysregulated ceRNAs were identified in Adriamycin, Cytoxan, and Tamoxifen. The interactions in these ceRNAs showed strong correlations in BRCA. Most drug response-related dysregulated ceRNAs were only present in one kind of drug. A number of drug response-related ceRNAs presented diverse dysregulation patterns. We also extracted some key drug response-related lncRNAs, such as HCP5 and FAM182A. These lncRNAs were associated with certain cancer hallmarks and survival in BRCA. Conclusions: Ultimately, understanding the underlying lncRNA-mediated ceRNAs in drug responses will facilitate improved individual reactions to chemotherapy and overall outcomes of BRCA treatment.
ZNF674-AS1 Suppresses Non-Small Cell Lung Cancer Growth Through the miR-423-3p/p21 Axis
Authors: Liu Yu , Huang Risheng , Xie Deyao , Lin Xiaoming , Zheng Liangcheng 
Published: August 2020

Abstract
Background: ZNF674-AS1, a recently characterized long noncoding RNA, shows prognostic significance in hepatocellualar carcinoma and glioma. However, the expression and function of ZNF674-AS1 in non-small cell lung cancer (NSCLC) is unclear. Methods: In this work, we investigated the expression of ZNF674-AS1 in 83 pairs of NSCLC specimens and adjacent noncancerous lung tissues. The clinical significance of ZNF674-AS1 in NSCLC was analyzed. The role of ZNF674-AS1 in NSCLC growth and cell cycle progression was explored. Results: Our data show that ZNF674-AS1 expression is decreased in NSCLC compared to normal tissues. ZNF674-AS1 downregulation is significantly correlated with advanced TNM stage and decreased overall survival of NSCLC patients. Overexpression of ZNF674-AS1 inhibits NSCLC cell proliferation, colony formation, and tumorigenesis, which is accompanied by a G0/G1 cell cycle arrest. Conversely, knockdown of ZNF674-AS1 enhances the proliferation and colony formation of NSCLC cells. Biochemically, ZNF674-AS1 overexpression increases the expression of p21 through downregulation of miR-423-3p. Knockdown of p21 or overexpression of miR-423-3p blocks ZNF674-AS1-mediated growth suppression and G0/G1 cell cycle arrest. In addition, ZNF674-AS1 expression is negatively correlated with miR-423-3p in NSCLC specimens. Conclusions: ZNF674-AS1 suppresses NSCLC growth by downregulating miR-423-3p and inducing p21. This work suggests the therapeutic potential of ZNF674-AS1 in the treatment of NSCLC.
CD4/CD8 ratio can predict the risk of septicemia in HIV infection
Authors: ZHU YINGCHUN , Zheng Ping , Cai Lin , Zhou Ruifeng , Wang Yin , Wang Zongzheng 
Published: August 2020

Abstract
Objectives

There are few reports about the effect of CD4/CD8 ratio on the infection of HIV with sepsis. Here we analyze the correlation between the baseline CD4 / CD8 ratio and the pathogenic spectrum of AIDS patients with sepsis and the effect on the prognosis.

Methods

A retrospective analysis study(May 2010 to June 2017) was carried out using routinely colleted data from HIV with spesis at Chengdu Public Health Clinical Center. The values of clinical data, CD4/CD8 ratio and pathogenic spectrum were evaluated in the study.

Results

Among 322 positive blood culture HIV patients with septicemia, 208 (64.60%) were fungal infection, 64 (19.90%) were gram-negative bacterial infection, 47 (14.60%) were Gram-positive bacterial infection, and 3 (0.90%) were fungal and bacterial infection. Among the fungal infections, Cryptococcus was found in 152 cases (73.08%), followed by cyanobacteria marneffei in 53 cases (25.48%) and Candida in 3 cases (1.44%); Salmonella in 38 cases (59.38%) and Escherichia coli in 17 cases (26.56%); Staphylococcus in 28 cases (59.58%) were positive. When the CD4 / CD8 ratio≤0.3, the risk of sepsis infection was significantly higher than that of CD4 / CD8 > 0.3. The lower the ratio of CD4 / CD8 (< 0.1), the higher the risk (χ 2 =188.968,P = 0.00).When the ratio of CD4 / CD8 was less than or equal to 0.3, the incidence of fungal infection was significantly increased (67.14%), and the incidence of gram-negative bacterial infection (18.02%) was significantly higher than that of Gram-positive bacterial infection (13.78%), (χ 2 = 283.277, P = 0.00); when the ratio of CD4 / CD8 was greater than or equal to 0.3, the incidence of septicemia was significantly reduced, and there was no significant difference between the original spectrum of infection (χ 2 = 1.750, P=0.42)。 286 patients (88.82%) were discharged after treatment, 36 patients (11.18%) died after treatment.

Conclusions

In Chengdu of China, the main pathogens of septicemia in AIDS patients are fungi, including Cryptococcus and cyanobacteria marneffei, next are bacteria containing Salmonella and Staphylococcus. The lower the ratio of CD4 / CD8, the higher the risk of infection with sepsis, the higher the risk of multiple infection, and the higher the mortality. Therefore, we think that CD4 / CD8 ratio is an important risk factor for HIV infection with septicemia. We should pay more attention to role of the CD4 / CD8 ratio in clinical work, and it may be help us predicting progress of HIV disease.
Relevance of Regional Lymph Node Invasion in Radical Hepatectomy and Lymphadenectomy for Alveolar Echinococcosis: A Retrospective Cohort Study
Authors: Yang Xianwei , Wang Tao , Shen Shu , Wang Wentao 
Published: August 2020

Abstract
Background Hepatic Alveolar echinococcosis (AE) is also known as worm cancer, parasitic cancer, and it often invades the regional lymph nodes of the liver. The present study was to investigate the clinical value of radical hepatectomy and lymphadenectomy in AE patients. Methods Our study enrolled consecutive AE patients who underwent radical hepatectomy with removal the regional lymph nodes between January 2009 and April 2019. Patients with inflammatory lymph node enlargement was included in group A, and patients with AE lymph node invasion were included in group B. The clinical characteristics, survival and recurrence rates were compared.Results A total of 103 patients were enrolled group A, and 24 in group B. Preoperative computed tomography showed that the lymph node positivity rate in group B was 70.8% while that in group A was 43.7% (p=0.017). The lymph node diameter was 1.8±0.9 cm in group A vs 2.5±1.1 cm in group B (p=0.004), and the lymph node number (p=0.035) and lymph node location (p=0.001). A total of 10 patients (7.9%) had recurrent lesions, and 6 patients (4.7%) died (P>0.05). Conclusions lymph node diameter, number, and distance from the liver were important characteristics for describing lymph node invasion. There was no difference in the long-term efficacy of lymphadenectomy between patients with lymph node enlargement and those with invasion.
Тернопольский национальный медицинский университет имени И.Я. Горбачевского Министерства здравоохранения Украины, г. Тернополь, Украина
Authors: Kovalchuk T.A. , Boyarchuk O.R. 
Published: August 2020

Актуальность. Результаты последних научных исследований позволяют предположить, что витамин D может оказывать косвенное, а в некоторых случаях и прямое действие в патогенезе вазовагальных синкопе у детей. Цель исследования: изучить уровни витамина D в сыворотке крови детей с вазовагальными синкопе. Материалы и методы. Основную группу составили 30 детей в возрасте 8–17 лет с как минимум одним эпизодом вазовагального синкопе в течение последнего месяца. В контрольную группу вошли 24 практически здоровых ребенка. 25(ОН)D в сыворотке крови определяли методом иммуноферментного анализа с использованием тест-системы Monobind (США). Оценку витамин-D-статуса осуществляли в соответствии с практическими рекомендациями по добавкам витамина D и лечению дефицита в Центральной Европе: дефицит — < 20 нг/мл; субоптимальный уровень (недостаточность) — 20–30 нг/мл; оптимальный уровень — 30–50 нг/мл; высокое содержание — 50–100 нг/мл; опасный уровень — > 100 нг/мл. Результаты. Дети обеих групп были сопоставимы по результатам демографических и клинических показателей, что позволило нивелировать влияние пола, возраста, дефицита и избыточной массы тела, ожирения или артериальной гипертензии на уровень 25(ОН)D. Показатель 25(ОН)D в группе вазовагальных синкопе составил 19,17 ± 1,33 нг/мл и был достоверно ниже по сравнению со здоровыми детьми — 30,91 ± 1,20 нг/мл (р = 0,000001). Оптимальный уровень витамина D регистрировали только у 6,7 % больных с вазовагальными синкопе, тогда как дефицит и недостаточность витамина D — у 33,3 и 60,0 %. Показатели кальция, фосфора и щелочной фосфатазы в сыворотке крови обследованных обеих групп не отличались (p > 0,05). Выводы. У 93,3 % детей с вазовагальными синкопе наблюдается достоверное снижение уровня 25(OH)D в сыворотке крови. Распространенность дефицита витамина D составляет 60,0 %, субоптимального уровня 25(ОН)D — 33,3 %. Дефицит и недостаточность витамина D ассоциируются с сохранением традиционных показателей кальций-фосфорного гомеостаза в пределах референтных значений.
Нарушения репродуктивной функции и гормонального профиля у пациенток с АКТГ-секретирующими аденомами гипофиза
Authors: Halimova Z.Yu. , Irgasheva O.B. 
Published: August 2020

Актуальность. Синдром Кушинга (СК) — тяжелая нейроэндокринная патология, которая уже многие годы находится под пристальным вниманием различных специалистов вследствие разноплановости ее осложнений. Гиперандрогения и нарушения менструального цикла, такие как аменорея и олигоменорея, дополняют клиническую картину СК. Цель исследования: оценить состояние фертильной функции и гормонального профиля у пациенток с синдромом Кушинга. Материалы и методы. Под наблюдением по поводу СК находились в период с 2000 по 2019 г. 163 пациентки фертильного возраста. Средний возраст обследуемых составил 27,58 ± 3,40 года (от 17 до 49 лет), длительность заболевания — 4,2 ± 0,2 года. 20 здоровых женщин соответствующего возраста составили группу контроля. Результаты. Пациентки с СК были распределены следующим образом: первая группа — с АКТГ-зависимым СК — 130 (79,7 %), вторая группа — с АКТГ-независимым СК — 30 (18,4 %) и третья группа — с АКТГ-эктопированным СК — 3 (1,8 %). Среди различных репродуктивных нарушений у пациенток с СК преобладали нарушения менструального цикла и вторичная аменорея. Для пациенток первой группы на 14-й день менструального цикла характерным было достоверное снижение базальных уровней лютеинизирующего и фолликулостимулирующего гормонов, снижение уровня овариальных гормонов (эстрадиол, прогестерон) на фоне гиперандрогенемии и достоверное повышение уровня кортизола крови при увеличении показателя АКТГ. Гиперплазия обоих надпочечников диагностирована у 110 (84,6 %) больных с АКТГ-зависимым СК, узелковая гиперплазия обоих надпочечников — у 13 (10 %), инциденталома — у 3 (2,3 %), узелковая гиперплазия одного надпочечника — у 2 (1,5 %). Выводы. У пациенток с синдромом Кушинга наблюдались репродуктивные расстройства: нарушение менструального цикла — у 32,9 %, вторичная аменорея — у 20,4 %. Большая часть нарушений менструального цикла установлена в группе больных с АКТГ-зависимым синдромом Кушинга. Нарушения менструального цикла проявлялись в виде олигоменореи (30 % случаев), аменореи (35 %), полименореи (7,5 %) и вариабельности цикла (7,5 %).
Дисфункция щитовидной железы и сердечно-сосудистые заболевания: состояние проблемы, пути решения
Authors: Netyazhenko V.Z. , Liakhotska A.V. 
Published: August 2020

Заболевания щитовидной железы широко распространены и являются наиболее частой эндокринной патологией, особенно в регионах с недостаточным содержанием йода в окружающей среде. Дисфункция щитовидной железы диагностируется у 5–10 % населения, чаще у женщин и в возрастной группе старше 60 лет. С увеличением возраста возникновение коморбидных заболеваний у пациентов значительно возрастает. Среди них первое место занимает патология сердечно-сосудистой системы. В качестве одного из факторов можно выделить эндотелиальную дисфункцию, которая является одним из предикторов морфологических изменений сосудистой стенки при многих хронических заболеваниях, в частности при атеросклерозе, артериальной гипертензии, заболеваниях щитовидной железы и др.
METHOD OF NAHWU IN ARABIC LEARNING
Authors: M. Asy'ari M. Asy'ari 
Published: August 2020

Language is very important and the impression that Nahwu is a difficult science to learn, especially by Muslims. As one of the basic sciences in Arabic, Nahwu's science cannot be ignored because without Nahwu's knowledge, Arabic will become chaotic and the order of words and sentences will be disorganized. Therefore, in learning Arabic, it is important to know Nahwu's knowledge, even though this method of teaching science is quite widely practiced by nahwu teachers, but students continue to face difficulties in learning it. Therefore, it is necessary to use suitable teaching methods and appropriate steps and subject matter that must be prioritized to be taught to students, so as to facilitate them in learning Nahwu science. The function of the learning method is one tool to achieve the goal. By using methods accurately the teacher will be able to achieve the learning objectives. When goals are formulated so that students have certain skills, the methods used must be adapted to the goals. Language learning methods, including Arabic, have evolved over time along with the development of the underlying theories, the results of research and experimentation in language teaching, as well as the development of the demands of the community's needs in the language field.
لترقية قدرة الطالبات على فهم المقروء بمعهد إنسان قرآني آتشيه بسار Quantum Reading تطبيق أسلوب
Authors: Izzah Febilia Nurul , Muslim Buhori , Ninoersy Tarmizi 
Published: August 2020

Reading is an important element in the process of learning Arabic and is one of the language skills. Reading helps students to absorb knowledge and increase interest in writing. Then qiraah can improve students' knowledge with vocabulary, sentences, and editors that can be used in speaking and writing skills. The fahmul maqruk is one of the subjects in the Insan Qur'ani boarding school which aims to enrich students in understanding the reading text. Through observations that the researchers did, it was found that second grade students of the senior high school did not yet master Arabic, especially reading skills. They can read Arabic texts, but do not understand it and are they are unable to answer quetions in the book text. Although the teacher has explained them some of the vocubulary in the book related to the text. This study aims to find out response of teacher and students at Insan Qur’ani Islamic Boarding School in teaching the language using this model and to find out the effectiveness of Quantum Reading model implementation to improve students in reading comprehension. The research method of this study is experimental research. Researcher conducted this study in a group called “One Group Pre-Test Post Test Design”. The result of this research is the teacher and students response in reading teaching using this model is excellent with the achievement of observation value of teacher activity is P = 87,5% between 81-100% with excellent category and observation value of students activity is P = 82,5% between 81-100% with excellent category too. And the aplication of this model effectuve to improve students’ ability in reading comprehension, the proof is that the value of T-test with p -value sig. (2-tailed) is smaller than the value of alpha ⍺ = 0,05 or (T-test < ⍺ = 0,05) with value is (0,000 < 0,05).
GERENCIAMENTO DO ABSENTISMO DE CURTO PRAZO NO LOCAL DE TRABALHO
Authors: Alves de Castro Charles 
Published: August 2020

This report outlines and discusses the insights on how to manage shot-term absenteeism within the workplace, as well as point out themes related to human resource management. Additionally, management considerations have been discussed such as “Fit Notes” and “Presenteeism”. As well as, a focus was placed on additional areas of concern within the U.S.A. in the form of bullying and harassment. Finally, was conducted a survey method within a class at the University of Limerick where 100% of the sample are workers. Therefore, the trends and patterns section outlined a Class Survey, methods for measuring absenteeism and triggers associated with the concept.
The Effect of Tourists' Motivations on Cognitive, Affective, and Unique Image of Bali
Authors: Prameswari Alita , Honantha Christina Rahardja , Anandya Dudi 
Published: August 2020

This research examines the effect of tourists’ motivations on cognitive, affective and unique image of Bali. The model used for this study adopted from Llodra-Riere, et al. (2015). The variable of the study consists of information source, motivation, cognitive image, affective image and unique image. The model in this study used six hypotheses. This research is quantitative and causal type research. This study used non-probability and purposive sampling approach, sample characteristics are the Surabaya tourists who had experience in using information sources from internet platform and other information sources which is non-internet platform that been collected information before visiting Bali and have visited Bali at least once in the past one year. The data is obtained from questionnaire that is distributed to 310 respondents and analyze using Structural Equation Modeling (SEM), with SPSS 18 and LISREL 8.8 for measurement and structural model. The result shows that motivation did not influence affective image of Bali. There are five hypotheses are significant. Information sources influence motivation; cognitive image and unique image influence affective image; motivation influences cognitive image and unique image of Bali. The benefit of this research can help tourism industry in Bali to improve their marketing communication by using social media, website platform and online advertisement.
Relationship between extreme obesity and mortality in patients with reduced ejection fraction
Authors: Siegel Robert M. , Ostfeld Robert J. , Aboodi Michael S. , Vittorio Timothy J. , Mehlman Yonatan , Travin Mark I. 
Published: August 2020

Aims
Although obesity is associated with increased mortality, epidemiologic studies in heart failure have reported lower mortality in obese patients compared with matched nonobese patients (the ‘obesity paradox’). However, the relationship between survival and extreme (morbid) obesity (BMI ≥ 40) is poorly understood. We evaluate survival in low ejection fraction patients across a range of BMI categories, including extreme obesity.

Methods
In a retrospective review, 12 181 consecutive patients receiving nuclear stress testing at a tertiary care center were stratified based on BMI and ejection fraction. Eight-year mortality data were collected using the social security death index.

Results
Normal ejection fraction patients (internal control, ejection fraction ≥50%) exhibited the J-shaped association between mortality and BMI that is observed in the general population. Among patients with reduced ejection fraction (<50%), survival improved as obesity increased (P < 0.0001). Those with extreme obesity had the lowest mortality (n = 1134, P < 0.05).

Conclusion
In this cohort of reduced Ejection fraction patients, the obesity paradox was observed in all weight categories, with the highest survival of all observed in the extremely obese BMI category. This further supports hypotheses that an obesity-related physiologic phenomenon affects mortality in reduced ejection fraction patients.