bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wie lässt sich die Eliminierung von Hepatitis B, C und D in Deutschland messen? Ergebnisse eines interdisziplinären Arbeitstreffens
Authors:   die Hepatitis-Monitoring-Arbeitsgruppe, Zimmermann Ruth , Külper-Schiek Wiebe , Steffen Gyde , Gillesberg Lassen Sofie , Bremer Viviane , Dudareva Sandra 
Published: December 2020

Zusammenfassung
Hintergrund
Die Weltgesundheitsorganisation (WHO) hat 2016 eine Strategie zur Eliminierung von Hepatitis-B-, -C- und -D-Virusinfektionen verfasst und Indikatoren zum Monitoring des Fortschritts definiert. Das Robert Koch-Institut hat 2019 ein interdisziplinäres Arbeitstreffen zur Verbesserung der Datenlage veranstaltet.

Ziele
Ziele waren die Vernetzung der Akteure, die Erstellung einer Übersicht zu den in Deutschland vorhandenen Datenquellen zu Hepatitis B, C und D und die Diskussion methodischer Aspekte.

Material und Methoden
Die für Deutschland relevanten WHO-Indikatoren wurden extrahiert und es wurde bestimmt, wie diese anhand vorliegender Daten konstruiert werden können. Bei dem Arbeitstreffen mit AkteurInnen aus dem öffentlichen Gesundheitsdienst, aus Kliniken, Laboren, von Krankenkassen, Forschungsinstituten, Datenhaltern und Registern wurden in Arbeitsgruppen Erhebungsmethoden diskutiert, welche dazu dienen können, fehlende Daten zu ermitteln. Die Datenquellen und Daten wurden hinsichtlich Qualität, Vollständigkeit sowie praktischer Umsetzbarkeit evaluiert und priorisiert.

Ergebnisse
Für die Allgemeinbevölkerung können die Indikatoren zu Prävention, Testung, Diagnose, Behandlung, Heilung, Folgeschäden und Mortalität aus Diagnose‑, Versorgungs- und Registerdaten, Daten aus Laboren und klinischen Zentren sowie einzelnen Studien konstruiert werden. Datenquellen für vulnerable Gruppen beschränken sich auf einzelne Studien zu Drogengebrauchenden, Männern, die Sex mit Männern haben, und HIV-Ko-Infizierten. Daten für MigrantInnen, Inhaftierte und SexarbeiterInnen sind kaum verfügbar; ebenso fehlen aktuelle Daten zur Krankheitslast chronischer Hepatitisinfektionen in der Allgemeinbevölkerung.

Diskussion
Für alle ausgewählten Indikatoren konnten Datenquellen, ihre Besonderheiten und Limitationen identifiziert werden. Im nächsten Schritt gilt es, die entwickelten Ideen in konkrete Projekte mit einzelnen Datenhaltern umzusetzen.
Risikofaktoren für Krebserkrankungen im Kindes- und Jugendalter
Authors: Stanulla M. , Erdmann F. , Kratz C. P. 
Published: December 2020

ZusammenfassungDie Ursachen von Krebs im Kindes- und Jugendalter sind weitestgehend unbekannt. Beispiele für konsistent beobachtete Risikofaktoren, die in umfangreichen epidemiologischen Untersuchungen identifiziert wurden, sind Strahlenexposition, Chemotherapie, niedriges und hohes Geburtsgewicht, höheres Alter der Mutter, Geschlecht und das Vorhandensein angeborener Anomalien. Diese Faktoren erklären jedoch nur einen geringen Anteil aller inzidenten Fälle. Die derzeit vielversprechendsten Ansätze zur Verbesserung des Verständnisses über die Ursachen von Krebserkrankungen im Kindes- und Jugendalter stammen von genetischen Analysen. Abgesehen von den durch genomweite Assoziationsstudien identifizierten Allelen mit geringem Krebsrisiko wurde festgestellt, dass seltene Keimbahnvarianten, die Krebsprädispositionsyndrome verursachen, mit einem erhöhten Risiko für die Entwicklung von Krebs im Kindes- und Jugendalter verbunden sind. Dieser Beitrag fasst das aktuelle Wissen über die exogenen und endogenen Ursachen von Krebserkrankungen in den frühen Lebensjahren zusammen und gibt einen Ausblick darauf, wie stetig wachsendes Wissen in diesem Bereich zukünftige präventive, diagnostische und therapeutische Strategien beeinflussen könnte.
Design and overview of the Origins of Alzheimer’s Disease Across the Life course (ORACLE) study
Authors: Lamballais Sander , Adank Maria C. , Hussainali Rowina F. , Schalekamp-Timmermans Sarah , Vernooij Meike W. , Luik Annemarie I. , Steegers Eric A. P. , Ikram Mohammad Arfan 
Published: December 2020

AbstractBrain development and deterioration across the lifespan are integral to the etiology of late-life neurodegenerative disease. Factors that influence the health of the adult brain remain to be elucidated and include risk factors, protective factors, and factors related to cognitive and brain reserve.
To address this knowledge gap we designed a life-course study on brain health, which received funding through the EU ERC Programme under the name Origins of Alzheimer’s Disease Across the Life course (ORACLE) Study. The ORACLE Study is embedded within Generation R, a prospective population-based cohort study of children and their parents, and links this with the Rotterdam Study, a population-based study in middle-aged and elderly persons. The studies are based in Rotterdam, the Netherlands. Generation R focuses on child health from fetal life until adolescence with repeated in-person examinations, but has also included data collection on the children’s parents. The ORACLE Study aims to extend the parental data collection in nearly 2000 parents with extensive measures on brain health, including neuroimaging, cognitive testing and motor testing. Additionally, questionnaires on migraine, depressive symptoms, sleep, and neurological family history were completed. These data allow for the investigation of longitudinal influences on adult brain health as well as intergenerational designs involving children and parents. As a secondary focus, the sampling is enriched by mothers (n = 356) that suffered from hypertensive disorders during pregnancy in order to study brain health in this high-risk population. This article provides an overview of the rationale and the design of the ORACLE Study.
Relative Deprivation and Hope: Predictors of Risk Behavior
Authors: Keshavarz Shahriar , Coventry Kenny R. , Fleming Piers 
Published: December 2020

AbstractThe belief that one is in a worse situation than similar others (Relative Deprivation) has been associated with involvement in a range of maladaptive escape behaviors, including excessive risk taking. Yet not everyone scoring high on measures of relative deprivation makes maladaptive choices. We hypothesized thathopemay ameliorate the negative effects of relative deprivation. In two laboratory-based experiments using a novel risk-taking task (N = 101) we show that hope reduces risk-taking behavior in relatively deprived participants. A third study (N = 122) extended the moderating effect of hope on relative deprivation to real-world risk behavior; increased hope was associated with decreased likelihood of loss of control of one’s gambling behavior in relatively deprived individuals. Nurturing hope in relatively deprived populations may protect them against maladaptive behaviors with potential applications for harm reduction.
Cryostratigraphy of mid-Miocene permafrost at Friis Hills, McMurdo Dry Valleys of Antarctica
Authors: Verret Marjolaine , Dickinson Warren , Lacelle Denis , Fisher David , Norton Kevin , Chorley Hannah , Levy Richard , Naish Tim 
Published: December 2020

AbstractThe origin and stability of ground ice in the stable uplands of the McMurdo Dry Valleys remains poorly understood, with most studies focusing on the near-surface permafrost. The 2016 Friis Hills Drilling Project retrieved five cores reaching 50 m depth in mid-Miocene permafrost, a period when Antarctica transitioned to a hyper-arid environment. This study characterizes the cryostratigraphy of arguably the oldest permafrost on Earth and assesses 15 Myr of ground ice evolution using the REGO model. Four cryostratigraphic units were identified: 1) surficial dry permafrost (0–30 cm), 2) ice-rich to ice-poor permafrost (0.3–5.0 m) with high solute load and δ18O values (-16.2 ± 1.8‰) and low D-excess values (-65.6 ± 4.3‰), 3) near-dry permafrost (5–20 m) and 4) ice-poor to ice-rich permafrost (20–50 m) containing ice lenses with low solute load and δ18O values (-34.6 ± 1.2‰) and D-excess of 6.9 ± 2.6‰. The near-surface δ18O profile of ground ice is comparable to other sites in the stable uplands, suggesting that this ice is actively responding to changing surface environmental conditions and challenging the assumption that the surface has remained frozen for 13.8 Myr. The deep ice lenses probably originate from the freezing of meteoric water during the mid-Miocene, and their δ18O composition suggests mean annual air temperatures ~7–11°C warmer than today.
Isolated right subclavian artery in a left aortic arch with ventricular septal defect
Authors: Shati Ayed A. 
Published: December 2020

AbstractIsolated origin of the subclavian artery is a congenital anomaly of the aortic arch in which one subclavian artery is attached to the ipsilateral pulmonary artery through ligamentous arteriosus. An isolated right subclavian artery with the left-sided aortic arch is an extremely rare condition. We report on an asymptomatic 2-year-old-girl, who was referred because of an incidental cardiac a murmur. She was diagnosed by echocardiography to have an isolated right subclavian artery connected to the right pulmonary artery in a left aortic arch with a ventricular septal defect. MRI confirmed the findings.
The expectations of transgender people in the face of their health-care access difficulties and how they can be overcome. A qualitative study in France
Authors: Allory Emmanuel , Duval Ellie , Caroff Marion , Kendir Candan , Magnan Raphaël , Brau Bernard , Lapadu-Hargue Elinore , Chhor Sidonie 
Published: December 2020

Abstract

Aim:
Our objective was to explore the difficulties experienced by transgender people in accessing primary health-care services and their expectations towards primary care providers to improve their health-care access.

Background:
Because transgender people are exposed to many discriminations, their health-care access is particularly poor. Guidelines recommend greater involvement of primary care providers in the processes because of the accessibility feature of primary care services.

Methods:
A qualitative study using semi-directed interviews was conducted among 27 transgender people (February 2018 – August 2018). These voluntary participants were recruited through different means: local trans or LGBTI (lesbian, gay, bisexual, trans, and/or intersex) associations, primary care providers, and social networks. The data analysis was based on reflexive thematic analysis in an inductive approach.

Findings:
Difficulties in accessing health-care occurred at all the levels of the primary health-care system: primary care providers – transgender people interaction, access to the primary care team facility (starting with the secretariat), access to secondary care specialists, and continuity of care. Transgender people report ill-adapted health-care services as a result of gender-based identification in health-care settings. Their main expectation was depsychiatrization and self-determination. They supported mixed health network comprising primary care providers and transgender people with a coordinating role for the general practitioner. These expectations should be priorities to consider in our primary health-care system to improve access to health-care for transgender people.
An Analytical Four‐Dimensional Ensemble‐Variational Data Assimilation Scheme
Authors: Liang Kangzhuang , Li Wei , Han Guijun , Shao Qi , Zhang Xuefeng , Zhang Liang , Jia Binhe , Bai Yang , Liu Siyuan , Gong Yantian 
Published: December 2020

AbstractThe usage of four‐dimensional variational (4DVar) scheme is limited by the static background error covariance and the adjoint model. In a hybrid frame of the four‐dimensional ensemble‐variational data assimilation scheme (4DEnVar), being able to avoid the tangent linear and adjoint models in the 4DVar and nowadays developed into a cutting‐edge research topic of the next‐generation data assimilation methods, an analytical 4DEnVar (A‐4DEnVar) scheme is designed. First, an analytical expression for explicit evolution of the background error covariances is derived. The expression collects the innovation of observations over an assimilation window simultaneously and propagates information to the initial background field by temporal cross covariances. Second, to estimate the adjoint model, the temporal covariances are constructed with ensemble members being centralized with respect to the model states integrated from the initial condition. Third, an iterative linear search process is introduced to minimize the cost function to update the analysis field until convergence. Twin experiments based on the Lorenz chaos model with three variables are conducted for the validation of the A‐4DEnVar scheme. Comparisons to the conventional 4DVar show that the A‐4DEnVar is comparable in accuracy even with a long assimilation window and sparse observations. The assimilation results also show that the A‐4DEnVar scheme can be implemented with a very small ensemble size which means that under circumstances without the tangent linear and adjoint models it can be easily incorporated into data assimilation systems in use.
Computed tomography chest imaging offers no advantage over chest X-ray in the initial assessment of gestational trophoblastic neoplasia
Authors: Parker Victoria L. , Winter Matthew C. , Whitby Elspeth , Parker William A. E. , Palmer Julia E. , Tidy John A. , Pacey Allan A. , Hancock Barry W. , Harrison Robert F. 
Published: December 2020

Abstract
Background
The International Federation of Gynaecology and Obstetrics (FIGO) score identifies gestational trophoblastic neoplasia (GTN) patients as low- or high-risk of single-agent chemotherapy resistance (SACR). Computed tomography (CT) has greater sensitivity than chest X-ray (CXR) in detecting pulmonary metastases, but effects upon outcomes remain unclear.

Methods
Five hundred and eighty-nine patients underwent both CXR and CT during GTN assessment. Treatment decisions were CXR based. The number of metastases, risk scores, and risk category using CXR versus CT were compared. CT-derived chest assessment was evaluated as impact upon treatment decision compared to patient outcome, incidence of SACR, time-to-normal human chorionic gonadotrophin hormone (TNhCG), and primary chemotherapy resistance (PCR).

Results
Metastasis detection (p < 0.0001) and FIGO score (p = 0.001) were higher using CT versus CXR. CT would have increased FIGO score in 188 (31.9%), with 43 re-classified from low- to high-risk, of whom 23 (53.5%) received curative single-agent chemotherapy. SACR was higher when score (p = 0.044) or risk group (p < 0.0001) changed. Metastases on CXR (p = 0.019) but not CT (p = 0.088) lengthened TNhCG. Logistic regression analysis found no difference between CXR (area under the curve (AUC) = 0.63) versus CT (AUC = 0.64) in predicting PCR.

Conclusions
CT chest would improve the prediction of SACR, but does not influence overall treatment outcome, TNhCG, or prediction of PCR. Lower radiation doses and cost mean ongoing CXR-based assessment is recommended.
Epithelial loss of mitochondrial oxidative phosphorylation leads to disturbed enamel and impaired dentin matrix formation in postnatal developed mouse incisor
Authors: Imhof Thomas , Rosenblatt Katharina , Pryymachuk Galyna , Weiland Daniela , Noetzel Nicolas , Deschner James , Baris Olivier R. , Kimoloi Sammy , Koch Manuel , Wiesner Rudolf J. , Korkmaz Yüksel 
Published: December 2020

AbstractThe formation of dentin and enamel matrix depends on reciprocal interactions between epithelial-mesenchymal cells. To assess the role of mitochondrial function in amelogenesis and dentinogenesis, we studied postnatal incisor development in K320E-TwinkleEpi mice. In these mice, a loss of mitochondrial DNA (mtDNA), followed by a severe defect in the oxidative phosphorylation system is induced specifically in Keratin 14 (K14+) expressing epithelial cells. Histochemical staining showed severe reduction of cytochrome c oxidase activity only in K14+ epithelial cells. In mutant incisors, H&E staining showed severe defects in the ameloblasts, in the epithelial cells of the stratum intermedium and the papillary cell layer, but also a disturbed odontoblast layer. The lack of amelogenin in the enamel matrix of K320E-TwinkleEpi mice indicated that defective ameloblasts are not able to form extracellular enamel matrix proteins. In comparison to control incisors, von Kossa staining showed enamel biomineralization defects and dentin matrix impairment. In mutant incisor, TUNEL staining and ultrastructural analyses revealed differentiation defects, while in hair follicle cells apoptosis is prevalent. We concluded that mitochondrial oxidative phosphorylation in epithelial cells of the developed incisor is required for Ca2+ homeostasis to regulate the formation of enamel matrix and induce the differentiation of ectomesenchymal cells into odontoblasts.
Morphological effect of side chain on H3O+ transfer inside polymer electrolyte membranes across polymeric chain via molecular dynamics simulation
Authors: Cha JinHyeok 
Published: December 2020

AbstractPerformance and durability of polymer electrolyte membrane are critical to fuel cell quality. As fuel cell vehicles become increasingly popular, membrane fundamentals must be understood in detail. Here, this study used molecular dynamic simulations to explore the morphological effects of perfluorosulfonic acid (PFSA)-based membranes on ionic conductivity. In particular, I developed an intuitive quantitative approach focusing principally on hydronium adsorbing to, and desorbing from, negatively charged sulfonate groups, while conventional ionic conductivity calculations featured the use of mean square displacements that included natural atomic vibrations. The results revealed that shorter side-chains caused more hydroniums to enter the conductive state, associated with higher ion conductivity. In addition, the hydronium path tracking showed that shorter side-chains allowed hydroniums to move among host groups, facilitating chain adsorption, in agreement with a mechanism suggested in earlier studies.
Hemocompatibility of new magnetically-levitated centrifugal pump technology compared to the CentriMag adult pump
Authors: Schibilsky David , Takatani Setsuo , Schibilsky Barbara , Graf Tobias , da Silva Diana Michels , Wendel Hans Peter , Avci-Adali Meltem , Schlensak Christian 
Published: December 2020

AbstractThe specific hemocompatibility properties of mechanical-circulatory-support (MCS)-pump technologies have not previously been described in a comparable manner. We thus investigated the hemocompatibility-indicating marker of a new magnetically-levitated (MagLev) centrifugal pump (MT-Mag) in a human, whole-blood mock-loop for 360 min using the MCS devices as a driving component. We compared those results with the CentriMag adult (C-Mag) device under the same conditions according to ISO10993-4. Blood samples were analyzed via enzyme-linked-immunosorbent-assay (ELISA) for markers of coagulation, complement system, and the inflammatory response. The time-dependent activation of the coagulation system was measured by detecting thrombin-anti-thrombin complexes (TAT). The activation of the complement system was determined by increased SC5b-9 levels in both groups. A significant activation of neutrophils (PMN-elastase) was detected within the C-Mag group, but not in the MT-Mag group. However, the amount of PMN-elastase at 360 min did not differ significantly between groups. The activation of the complement and coagulation system was found to be significantly time-dependent in both devices. However, coagulation activation as determined by the TAT level was lower in the MT-Mag group than in the C-Mag group. This slight disparity could have been achieved by the optimized secondary flow paths and surface coating, which reduces the interaction of the surface with blood.
Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein
Authors: Kumari Priyanka , Kumar Jitesh , Kumar Ravi Ranjan , Ansar Mohammad , Rajani Kumari , Kumar Sunil , Ranjan Tushar 
Published: December 2020

AbstractViruses cause many severe plant diseases, resulting in immense losses of crop yield worldwide. Therefore, developing novel approaches to control plant viruses is crucial to meet the demands of a growing world population. Recently, RNA interference (RNAi) has been widely used to develop virus-resistant plants. Once genome replication and assembly of virion particles is completed inside the host plant, mature virions or sometimes naked viral genomes spread cell-to-cell through plasmodesmata by interacting with the virus-encoded movement protein (MP). We used the RNAi approach to suppress MP gene expression, which in turn prevented potato leafroll virus (PLRV) systemic infection in Solanum tuberosum cv. Khufri Ashoka. Potato plants agroinfiltrated with MP siRNA constructs exhibited no rolling symptoms upon PLRV infection, indicating that the silencing of MP gene expression is an efficient method for generating PLRV-resistant potato plants. Further, we identified novel ATPase motifs in MP that may be involved in DNA binding and translocation through plasmodesmata. We also showed that the ATPase activity of MP was stimulated in the presence of DNA/RNA. Overall, our findings provide a robust technology to generate PLRV-resistant potato plants, which can be extended to other species. Moreover, this approach also contributes to the study of genome translocation mechanisms of plant viruses.
Appropriate time to apply control input to complex dynamical systems
Authors: Ebrahimi Ali , Nowzari-Dalini Abbas , Jalili Mahdi , Masoudi-Nejad Ali 
Published: December 2020

AbstractControlling a network structure has many potential applications many fields. In order to have an effective network control, not only finding good driver nodes is important, but also finding the optimal time to apply the external control signals to network nodes has a critical role. If applied in an appropriate time, one might be to control a network with a smaller control signals, and thus less energy. In this manuscript, we show that there is a relationship between the strength of the internal fluxes and the effectiveness of the external control signal. To be more effective, external control signals should be applied when the strength of the internal states is the smallest. We validate this claim on synthetic networks as well as a number of real networks. Our results may have important implications in systems medicine, in order to find the most appropriate time to inject drugs as a signal to control diseases.
Effects of different irrigation protocols on dentin surfaces as revealed through quantitative 3D surface texture analysis
Authors: Elbahary Shlomo , Haj-yahya Sohad , Khawalid Majd , Tsesis Igor , Rosen Eyal , Habashi Waseem , Pokhojaev Ariel , Sarig Rachel 
Published: December 2020

AbstractThe combination of ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite (NaOCl) has been advocated as an effective irrigation methodology to remove organic and inorganic matter in root canal therapy. Yet, it was suggested that both solutions might lead to structural changes of the dentinal wall surface, depending on the order of application which might affect sealer mechanical retention. This study aims to evaluate the effect of different irrigating protocols on dentin surface roughness using quantitative 3D surface texture analysis. Data stems from 150 human root dentin sections, divided into five groups, each prepared according to one of the following protocols: Negative control; 17% EDTA; 17% EDTA followed by 5.25% NaOCl; 5.25% NaOCl; and 5.25% NaOCl followed by 17% EDTA. Each dentin sample was examined for its three-dimensional surface texture using a high-resolution confocal disc-scanning measuring system. EDTA 17% and the combined EDTA 17% with NaOCl 5.25% showed considerably higher roughness properties compared to the control and to NaOCl 5.25% alone. However, the irrigation sequence did not affect the dentin roughness properties. Therefore, mechanical retention is probably not dependent upon the selection of irrigation protocol sequence.