bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Influence of Perioperative Fluid Therapy on N-terminal-pro-brain Natriuretic Peptide and the Association With Heart and Lung Complications in Patients Undergoing Colorectal Surgery
Authors: Brandstrup Birgitte , Beier-Holgersen Randi , Iversen Lene H. , Starup Christian B. , Wentzel Loui N. , Lindorff-Larsen Karen , Petersen Troels C. , Tønnesen Hanne 
Published: December 2019

Objective:
To investigate the influence of intravenous (iv) fluid volumes on the secretion of N-terminal-pro-brain natriuretic peptide (NT-Pro-BNP) in colorectal surgical patients and its association with cardiopulmonary complications (CPC). In addition, to examine if preoperative NT-Pro-BNP can predict the risk for postoperative CPC.

Methods:
Blood samples from patients enrolled in a previously published clinical randomized assessor-blinded multicenter trial were analyzed.
1
Included were adult patients undergoing elective colorectal surgery with the American-Society-of-Anesthesiologists-scores of 1–3. Samples from 135 patients were available for analysis. Patients were allocated to either a restrictive (R-group) or a standard (S-group) iv-fluid regimen, commencing preoperatively and continuing until discharge. Blood was sampled every morning until the fourth postoperative day. The primary outcome for this study was NT-Pro-BNP changes and its association with fluid therapy and CPC.

Results:
The S-group received more iv-fluid than the R-group on the day-of-surgery [milliliter, median (range) 6485 (4401–10750) vs 3730 (2250–8510); P < 0.001] and on the first postoperative day. NT-Pro-BNP was elevated in the S-group compared with the R-group on all postoperative days [area under the curve: median (interquartile range) pg/mL: 3285 (1697–6179) vs 1290 (758–3719); P < 0.001 and in patients developing CPC vs no-CPC (area under the curve), median (interquartile range): 5196 (1823–9061) vs 1934 (831–5301); P = 0.005]. NT-pro-BNP increased with increasing fluid volumes all days (P < 0.003). Preoperative NT-Pro-BNP predicted CPC [odds ratio (confidence interval): 1.573 (0.973–2.541), P = 0.032; positive predictive value = 0.257, negative predictive value = 0.929].

Conclusions:
NT-pro-BNP increases with iv-fluid volumes given to colorectal surgical patients, and the level of NT-Pro-BNP is associated with CPC. Preoperative NT-Pro-BNP is predictive for CPC, but the diagnostic value is low.
Clinicaltrials.gov NCT03537989.
Wide Variation in Surgical Spending Within Hospital Systems
Authors: Chhabra Karan R. , Sheetz Kyle H. , Regenbogen Scott E. , Dimick Justin B. , Nathan Hari 
Published: December 2019

Objective:
We sought to measure the extent of variation in episode spending around total hip replacement within and across hospital systems.

Summary of Background Data:
Bundled payment programs are pressuring hospitals to reduce spending on surgery. Meanwhile, many hospitals are joining larger health systems with the stated goal of improved care at lower cost.

Methods:
Cross-sectional study of fee-for-service Medicare patients undergoing total hip replacement in 2016 at hospital systems identified in the American Hospital Association Annual Survey. We calculated risk- and reliability-adjusted average 30-day episode payments at the hospital and system level.

Results:
Average episode payments varied nearly as much within hospital systems ($2515 between the lowest- and highest-cost hospitals, 95% confidence interval $2272–$2,758) as they did between the lowest- and highest-cost quintiles of systems ($2712, 95% confidence interval $2545–$2879). Variation was driven by post-acute care utilization. Many systems have concentrated hip replacement volume at relatively high-cost hospitals.

Conclusions:
Given the wide variation in surgical spending within health systems, we propose tailored strategies for systems to maximize savings in bundled payment programs.
Prediction of Suicide and Nonfatal Self-harm After Bariatric Surgery: A Risk Score Based on Sociodemographic Factors, Lifestyle Behavior, and Mental Health
Authors: Konttinen Hanna , Sjöholm Kajsa , Jacobson Peter , Svensson Per-Arne , Carlsson Lena M. S. , Peltonen Markku 
Published: December 2019

Objective:
To identify preoperative sociodemographic and health-related factors that predict higher risk of nonfatal self-harm and suicide after bariatric surgery.

Background:
Evidence is emerging that bariatric surgery is related to an increased risk of suicide and self-harm, but knowledge on whether certain preoperative characteristics further enhance the excess risk is scarce.

Methods:
The nonrandomized, prospective, controlled Swedish Obese Subjects study was linked to 2 Nationwide Swedish registers. The bariatric surgery group (N = 2007, per-protocol) underwent gastric bypass, banding or vertical banded gastroplasty, and matched controls (N = 2040) received usual care. Participants were recruited from 1987 to 2001, and information on the outcome (a death by suicide or nonfatal self-harm event) was retrieved until the end of 2016. Subhazard ratios (sub-HR) were calculated using competing risk regression analysis.

Results:
The risk for self-harm/suicide was almost twice as high in surgical patients compared to control patients both before and after adjusting for various baseline factors [adjusted sub-HR = 1.98, 95% confidence interval (CI) = 1.34–2.93]. Male sex, previous healthcare visits for self-harm or mental disorders, psychiatric drug use, and sleep difficulties predicted higher risk of self-harm/suicide in the multivariate models conducted in the surgery group. Interaction tests further indicated that the excess risk for self-harm/suicide related to bariatric surgery was stronger in men (sub-HR = 3.31, 95% CI = 1.73–6.31) than in women (sub-HR = 1.54, 95% CI = 1.02–2.32) (P = 0.007 for adjusted interaction). A simple-to-use score was developed to identify those at highest risk of these events in the surgery group.

Conclusions:
Our findings suggest that male sex, psychiatric disorder history, and sleep difficulties are important predictors for nonfatal self-harm and suicide in postbariatric patients. High-risk patients who undergo surgery might require regular postoperative psychosocial monitoring to reduce the risk for future self-harm behaviors.
HomozygousSTAT2gain-of-function mutation by loss of USP18 activity in a patient with type I interferonopathy
Authors: Gruber Conor , Martin-Fernandez Marta , Ailal Fatima , Qiu Xueer , Taft Justin , Altman Jennie , Rosain Jéremie , Buta Sofija , Bousfiha Aziz , Casanova Jean-Laurent , Bustamante Jacinta , Bogunovic Dusan 
Published: December 2019

AbstractType I interferonopathies are monogenic disorders characterized by enhanced Type I interferon (IFN-I) activity. Inherited ISG15 and USP18 deficiencies underlie type I interferonopathies by preventing the regulation of late responses to IFN-I. Specifically, ISG15/USP18 are induced by IFN-I and sterically hinder JAK1 from binding to the IFNAR2 subunit of IFN-I receptor. We report an infant who died of autoinflammation due to a homozygous missense mutation (R148Q) inSTAT2. The variant is gain-of-function (GOF) for ISGF3-dependent induction of late but not early response to IFN-I. Surprisingly, the mutation does not enhance the intrinsic transcriptional activity of ISGF3. Rather, the STAT2 R148Q variant is GOF because it fails to appropriately interact with and traffic USP18 to IFNAR2, preventing USP18 from negatively regulating responses to IFN-I. Overall, a STAT2 missense mutation that fails to facilitate USP18-mediated signal termination in the homozygous state underlies a novel genetic etiology of type I interferonopathy.
Quorum sensing regulation inErwinia carotovoraaffects development ofDrosophila melanogasterinfected larvae
Authors: Vieira Filipe J. D. , Nadal-Jimenez Pol , Teixeira Luis , Xavier Karina B. 
Published: December 2019

AbstractMulti-host bacteria must rapidly adapt to drastic environmental changes, relying on integration of multiple stimuli for an optimal genetic response.Erwinia spp.are phytopathogens that cause soft-rot disease in plants.Erwinia carotovora Ecc15is used as a model for bacterial oral-route infection inDrosophila melanogasteras it harbors a gene, theErwiniavirulence factor (Evf), which has been previously shown to be a major determinant for infection ofD. melanogastergut. However, the factors involved in regulation ofevfexpression are poorly understood. We investigated whetherevfcould be controlled by quorum sensing since, in theErwiniagenus, quorum sensing regulates pectolytic enzymes, the major virulence factors needed to infect plants. Here, we show that transcription ofevfis positively regulated by quorum sensing inEcc15 viathe acyl-homoserine lactone (AHL) signal synthase ExpI, and the AHL receptors ExpR1 and ExpR2. Moreover, we demonstrate that the GacS/A two-component system is partially required forevfexpression. We also show that the load ofEcc15in the gut depends upon the quorum sensing-mediated regulation ofevf. Furthermore, we demonstrate that larvae infected withEcc15suffer a developmental delay as a direct consequence of the regulation ofevf viaquorum sensing. Overall, our results show thatEcc15relies on quorum sensing to control production of both pectolytic enzymes and Evf. This regulation influences the interaction ofEcc15with its two known hosts, indicating that quorum sensing and GacS/A signaling systems may impact bacterial disseminationviainsect vectors that feed on rotting plants.SignificanceIntegration of genetic networks allows bacteria to rapidly adapt to changing environments. This is particularly important in bacteria that interact with multiple hosts.Erwinia carotovora Ecc15is a plant pathogen that usesDrosophila melanogasteras a vector. To interact with these two hosts,Ecc15uses two different sets of virulence factors: plant cell wall-degrading enzymes to infect plants and theErwiniavirulence factor (evf) to infectDrosophila. Our work shows that, despite the virulence factors being different, both are regulated by homoserine lactone quorum sensing and the two component GacS/A system. Moreover, we show that these pathways are essential forEcc15loads in the gut ofDrosophilaand that this interaction carries a cost to the vector in the form of a developmental delay. Our findings provide evidence for the importance of quorum sensing regulation in the establishment of multi-host interactions.
Stone Alkaline Water Induces Apoptosis of Prostate Cancer Cells and Inhibits Tumor Cell Induced Angiogenesis In Vitro
Authors: Appak-Baskoy Sila , Sahin lknur Kulcanay , Teksoy Ozgun , Cengiz Mustafa , Ozkan Asuman Deveci , Eskiler Gamze Guney , Bilici Namık , Oztopcu-Vatan Pinar , Altuner Yılmaz , Ayhanci Adnan 
Published: December 2019

AbstractTreatment options to improve overall survival rate of prostate cancer patients are limited since tumor cells acquire resistance to the chemotherapeutic drugs. We aimed to determine anticancer effects of stone alkaline water (SAW) on PC-3 and DU-145 prostate adenocarcinoma cell lines. SAW was obtained by triturating high stones under vacuum at 3000 °C. High mineral and trace element containing fraction of SAW was used for the experiments. Viability of the tumor cells was analyzed using tetrazolium based WST-1 cell proliferation assay, cell cycle analysis was carried out with Propidum Iodide staining (Muse™ Cell Cycle Kit). Acridine Orange and Annexin V stainings were done to analyze the cellular morphology and to determine apoptosis. Tumor cell derived angiogenesis was analyzed with migration and tube formation assays. SAW treatment resulted in accumulation of cells at G0/G1 phase and inhibited tumor cell induced HUVEC tube formation and migration. SAW treatment significantly decreased viability of PC-3 and DU-145 prostate adenocarcinoma cells and induced apoptotic cell death. Intriguingly, treatment of the prostate cancer cells with SAW inhibited tumor cell derived angiogenesis. SAW may aid in treating prostate cancer and molecules important for SAW’s apoptotic and anti-angiogenic effects need to be determined.
Proteomics Analysis of Differential Expression of Lung Proteins in Response to Highly Pathogenic Avian Influenza Virus Infection in Chicken
Authors: Vijayakumar Periyasamy , Raut Ashwin Ashok , Chingtham Santhalembi , Murugkar Harshad V , Kulkarni Diwakar D. , Singh Vijendra Pal , Mishra Anamika 
Published: December 2019

AbstractElucidation of molecular pathogenesis underlying virus-host interaction is important for the development of new diagnostic and therapeutic strategies against highly pathogenic avian influenza (HPAI) infection in chicken. However, chicken HPAI viral pathogenesis is not completely understood. To elucidate the intracellular signaling pathways and critical host proteins associated with influenza pathogenesis, we characterized the lung proteome of chicken infected with HPAI H5N1 virus (A/duck/India/02CA10/2011/Agartala). The chicken mass spectra data sets comprised1, 47, 451 MS scans and 19, 917 MS/MS scans. At local FDR 5% level, we identified total 3313 chicken proteins with presence of at least one unique peptide. At 12 hrs, 247 proteins are downregulated while 1754 proteins are downregulated at 48 hrs indicating that the host has succumbed to infection. There is expression of proteins of the predominant signaling pathways, such as TLR, RLR, NLR and JAK-STAT signaling. Activation of these pathways is associated with cytokine storm effect and thus may be the cause of severity of HPAI H5N1 infection in chicken. Further we identified proteins like MyD88, IKBKB, IRAK4, RELA, and MAVS involved in the critical signaling pathways and some other novel proteins(HNF4A, ELAVL1, FN1, COPS5, CUL1, BRCA1 and FYN) as main hub proteins that might play important roles in influenza pathogenesis in chicken. Taken together, we characterized the signaling pathways and the proteomic determinants responsible for disease pathogenesis in chicken infected with HPAI H5N1 virus.
Precision in a sea of profusion: Myelin replacement triggered by single-cell cortical demyelination
Authors: Snaidero Nicolas , Schifferer Martina , Mezydlo Aleksandra , Kerschensteiner Martin , Misgeld Thomas 
Published: December 2019

SUMMARYMyelin—rather than being a static insulator of axons—is emerging as an active participant in circuit plasticity. This requires precise regulation of oligodendrocyte numbers and myelination patterns. Here, by devising a laser ablation approach of single oligodendrocytes, followed byin vivoimaging and correlated ultrastructural reconstruction, we show that in mouse cortex demyelination as subtle as loss of a single oligodendrocyte can trigger robust cell replacement and remyelination timed by myelin breakdown. This results in reliable reestablishment of the original myelin pattern along continuously myelinated axons, while in parallel profuse isolated internodes emerge on previously unmyelinated axons. Thus, in mammalian cortex, internodes along partially myelinated cortical axons are typically not re-established, suggesting that the cues that guide ‘patchy’ myelination are not preserved through cycles of de- and remyelination. In contrast, continuous ‘obligatory’ myelin shows remarkable homeostatic resilience with single axon precision.
Predation by Himalayan Wolves: Understanding conflict, culture and co-existence amongst Indo-Tibetan community and large carnivores in High Himalaya
Authors: Lyngdoh Salvador , Habib Bilal 
Published: December 2019

AbstractThe wolves in the Hindukush-Himalayan region belong to one the most basal lineages withinCanis lupus, yet little is known about its ecology, distribution, and behavior. To understand ecological aspects of wolves in this landscape, we predict wolf distribution, diet patterns and conflict perception in Spiti, India using field and remotely sensed information. We collected scats (n = 283) of canid species namely, Wolves, and other predators over a period of 3 years (2014-17) [66]. Wolf diet constituted mostly of domestic prey (79.02 %) while wild prey constituted to 17.80% of wolf diet over the three years. Village surveys recorded only 4% of the respondents confirmed wolf presence and perceived them as a possible threat to various livestock. Over, 98% of the respondents claimed that wolves were not safe for livestock and were averse to its presence. Marginal response curves depicted the model to have positive responses to animal location, LULC, village population, village density and wolf depredation. We found perceived presence/threat distribution wolves in the area significantly differed from actual ecological presence and distribution of wolves. The Himalayan wolf is an apex flagship predator in this fragile high altitude system, whose role is intricately linked with the ecology of the region. The use of such methods can aid in understanding such aspects as well as designing effective long-term conservation strategies for the species.
The genome polishing tool POLCA makes fast and accurate corrections in genome assemblies
Authors: Zimin Aleksey V. , Salzberg Steven L. 
Published: December 2019

AbstractThe introduction of third-generation DNA sequencing technologies in recent years has allowed scientists to generate dramatically longer sequence reads, which when used in whole-genome sequencing projects have yielded better repeat resolution and far more contiguous genome assemblies. While the promise of better contiguity has held true, the relatively high error rate of long reads, averaging 8–15%, has made it challenging to generate a highly accurate final sequence. Current long-read sequencing technologies display a tendency toward systematic errors, in particular in homopolymer regions, which present additional challenges. A cost-effective strategy to generate highly contiguous assemblies with a very low overall error rate is to combine long reads with low-cost short-read data, which currently have an error rate below 0.5%. This hybrid strategy can be pursued either by incorporating the short-read data into the early phase of assembly, during the read correction step, or by using short reads to “polish” the consensus built from long reads. In this report, we present the assembly polishing tool POLCA (POLishing by Calling Alternatives) and compare its performance with two other popular polishing programs, Pilon and Racon. We show that on simulated data POLCA is more accurate than Pilon, and comparable in accuracy to Racon. On real data, all three programs show similar performance, but POLCA is consistently much faster than either of the other polishing programs.
Interaction between satisfaction and incentives on online reviews
Authors: Dai Qi , Zhang Jingyi 
Published: December 2019

PurposeThe purpose of this paper is to investigate the interaction effect between customer satisfaction and monetary incentives on online reviews and test the moderating effect of personal characteristics, filling the research gap in online review behavior from the senders.Design/methodology/approachUsing a project role-playing technique that is widely applied in the marketing field, the authors conducted two experimental studies in a laboratory setting with student subjects and collected 390 and 362 acceptable samples for analysis in Studies 1 and 2, respectively.FindingsThis research confirms the positive effects of satisfaction and incentives on review scores and tests the interaction effect between satisfaction and incentives on review scores with the moderating effects of moral judgment and sensitivity of promotion. Incentives could strengthen customers’ review scores except under small incentives situation where dissatisfied customers decrease scores instead. Additionally, the moderating effects of moral judgment and sensitivity of promotion are more significant in the case of dissatisfaction.Research limitations/implicationsAs this study focuses exclusively on a single service context and uses student samples, limitations persist regarding the generalizability of the results.Practical implicationsThe research provides new insights for marketers on designing effective incentive programs, as well as how to better balance costs and benefits in promotion strategies.Originality/valueThis is one of the first studies to explore the interaction effect between satisfaction and incentives on online reviews considering the moderating effects of moral judgment and sensitivity of promotion. As a result, a new model is forwarded.
Lessons from the “BPO journey” in a public housing company: toward a strategy for BPO
Authors: Christiansson Marie-Therese , Rentzhog Olof 
Published: December 2019

PurposeDespite many efforts within organizations toward business process orientation (BPO), research on real-world experiences remains in its infancy. The purpose of this paper is to redress the existing knowledge gap by analyzing a Swedish public housing company that has made notable effects regarding BPO and to explore lessons learned from the BPO journey (from 1998 to 2013).Design/methodology/approachThe point of departure is principles in the BPO foundation, principles of successful BPM and effects in empirically based literature. The reconstruction of the narrative case study describes milestones and critical junctions, as well as effects based on quantitative and qualitative data.FindingsEffects in BPO are demonstrated in terms of higher customer satisfaction, increased innovative ability, improved operational performance, higher employee satisfaction and, as a result of these, increased profitability. Theoretical constructs with implications for the theory building on BPO are suggested in a three-layer management framework – with capabilities and abilities emerging from the case study used as an illustrative example.Practical implicationsLessons are learned regarding critical practices related to advancement in BPO. A strategy-building process based on eight design propositions is suggested to define the pre-conditions for BPO in an organization.Originality/valueThis is the first longitudinal case study to provide a comprehensive view and detailed insights of a BPO journey and top management performance toward a business process-oriented organization. Practitioners and BPM community get valuable insights into how the temporality and the context shape the BPO maturity process in terms of new organizational structure and roles during the journey.
Development of a time-variant causal model of human error in construction with dynamic Bayesian network
Authors: Ma Zhangming , Chong Heap-Yih , Liao Pin-Chao 
Published: December 2019

Purpose
Human error is among the leading causes of construction-based accidents. Previous studies on the factors affecting human error are rather vague from the perspective of complex and changeable working environments. The purpose of this paper is to develop a dynamic causal model of human errors to improve safety management in the construction industry. A theoretical model is developed and tested through a case study.

Design/methodology/approach
First, the authors defined the causal relationship between construction and human errors based on the cognitive reliability and error analysis method (CREAM). A dynamic Bayesian network (DBN) was then developed by connecting time-variant causal relationships of human errors. Next, prediction, sensitivity analysis and diagnostic analysis of DBN were applied to demonstrate the function of this model. Finally, a case study of elevator installation was presented to verify the feasibility and applicability of the proposed approach in a construction work environment.

Findings
The results of the proposed model were closer to those of practice than previous static models, and the features of the systematization and dynamics are more efficient in adapting toward increasingly complex and changeable environments.

Originality/value
This research integrated CREAM as the theoretical foundation for a novel time-variant causal model of human errors in construction. Practically, this model highlights the hazards that potentially trigger human error occurrences, facilitating the implementation of proactive safety strategy and safety measures in advance.