bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-Resolution Flow-Simulation in Typhoon 21, 2018: Massive Loss of Submerged Macrophytes in Lake Biwa
Authors: Nakada Satoshi , Haga Hiroki , Iwaki Maho , Hatano Keisuke , Mabuchi Kohji , Takamura Noriko 
Published: December 2020

Abstract
Global activities of typhoons and hurricanes are gradually changing, and these storms can drastically affect lake ecosystems through the recession of submerged macrophytes that regulate the water quality in lakes. Using an echosounder, we captured the short-term, massive loss of submerged macrophytes attributed to the abnormal fluctuation of the water level induced by the approach of a catastrophic super typhoon in the south basin of Lake Biwa, Japan. This paper investigates the physical processes responsible for the loss of vegetation using a high-resolution circulation model in Lake Biwa as a pilot study area. The circulation model was coupled with dynamical models of the fluid force and erosion acting on the vegetation. Our simulation successfully reproduced the water level fluctuation and high-speed current (torrent) generated by the typhoon gale. The simulated results demonstrate that the fluid force driven by the gale-induced torrent uprooted submerged macrophytes during the typhoon approach and that this fluid force (rather than erosion) caused the outflow of vegetation. As a result, this uprooting attributed to the fluid force induced the massive loss of submerged macrophytes in a large area of the south basin, which might have increased primary production and reduced the stock of fish such as bluegill in the lake. Our approach is practical for evaluating changes in lake environments attributed to the massive outflow of submerged macrophytes under various climate change scenarios. (227 words)
Malignant Tumor Is the Greatest Risk Factor for Pulmonary Embolism in Hospitalized Patients: A Single-Center Study
Authors: Fujieda Kaoru , Nozue Akiko , Watanabe Akie , Shi Keiko , Itagaki Hiroya , Hosokawa Yoshihiko , Nishida Keiko , Tasaka Nobutaka , Satoh Toyomi , Nishide Ken 
Published: December 2020

Abstract
BackgroundThis study aimed to investigate the background of patients who presented with pulmonary embolism (PE) on contrast-enhanced chest computed tomography (CT) and to explore the risk factors for PE.MethodsThis study included a review of the medical records of all 50,621 patients who were admitted to one community hospital between January 1, 2013 and December 31, 2017. Data on sex, age, risk factors related to blood flow stagnation (obesity, long-term bed rest, cardiopulmonary disease, cast fixation, long-term sitting), risk factors related to vascular endothelial disorder (surgery, trauma/fracture, central venous catheterization, catheter tests/treatments, vasculitis, antiphospholipid antibody syndrome, history of venous thromboembolism (VTE)), and risk factors related to hypercoagulability (malignant tumor, use of oral contraceptives/low-dose estrogen progestin/steroids, infection, inflammatory enteric disease, polycythemia, protein C or protein S deficiency, dehydration) were evaluated.ResultsOf all inpatients, 185 (0.37%) were diagnosed with PE after contrast-enhanced chest CT examination. The rate of discovering PE was significantly greater in women (0.48%, 93/19,409) than men (0.29%, 92/31,212) (P=0.0008). Of the 185 patients with PE, 173 (94%) had some type of risk factor. For both men and women, the most frequent risk factor was a malignant tumor, followed by infection and obesity for men and long-term bed rest and obesity for women. The most common malignant tumor was lung cancer. Of the 12 patients whose clear PE-inducing factor could not be identified, but there is possible involvement of antipsychotic agent.ConclusionsThe risk factors for PE were identified in this single-center, retrospective study.
Spatial and Temporal Distribution Changes of Human Brucellosis in Inner Mongolia, China, effects of Husbandry and Control Measures
Authors: Zhai Jingbo , zhang qi , Wang Ying , Wang Dali , Song Lihua , Chen Zeliang 
Published: December 2020

Abstract
BackgroundInner Mongolia has the highest incidence rate, causing great public health and economic losses. A number of policies to guide the prevention and control of human brucellosis had been issued and have made some achievements. Analysis of the distribution characteristics and changes of human brucellosis is important for the targeted prevention and control of this disease.MethodsWe collected various policies and measures related to brucellosis promulgated, and comprehensively analyzed the spatial and temporal distribution of human brucellosis in Inner Mongolia from 2005 to 2019 using the Bayesian theory of space-time modeling. ResultsWith the implementation of various policies and measures, great achievements have been made and distribution trends changed. Some western regions have changed into stable or cold-spot counties, and most cities in the eastern region have become hot-spot counties during the time period. The rising trend of risk in three cities is slowing down, while the risk in two cities is growing faster than the overall trend.ConclusionThe effective prevention and control measures are essential. The analysis results of this study may provide a theoretical and scientific basis for the public health department to develop targeted effective prevention and control measures for human brucellosis.
Over-expression of HSP70 induces apoptosis of intestinal epithelial cells in heat-stressed pigs: A proteomic approach
Authors: Li Junyu , Yong Yanhong , Yu Tianyue , Fang Biao , Wu Lianyun , Hu Canying , Liu Xiaoxi , Yu Zhichao , Ma Xingbin , Gooneratne Ravi , El-Aty A. M. Abd , Ju Xianghong 
Published: December 2020

Abstract
Background

Heat stress (HS)-induced intestinal epithelial cell apoptosis may play a pivotal role in intestinal barrier function injury in animals. However, the underlying molecular mechanism by which HS induces apoptosis of intestinal epithelial cells is still poorly understood.
Methods

The trial uses a prospective study. Firstly, a eukaryotic expression vector for the HSP70 gene was constructed and transfected into intestinal porcine epithelial cells (IPEC-J2) and then analyzed with a functional proteomics approach followed by liquid-chromatography-tandem mass spectrometry (LC-MS/MS) identification. Next, heat stress treatment of IPEC-J2 cells and pigs were performed. Immunoblotting and flow cytometry were used to examine the protein changes and cellular apoptosis.
Results

246 differentially expressed proteins (DEPs) were identified in HSP70-overexpressed IPEC-J2 cells, functional annotation suggested that most of the DEPs were related to ECM-receptor interaction, focal adhesion, and apoptosis. Moreover, apoptosis rate was increased in IPEC-J2 cells transfected with porcine HSP70 overexpressing plasmid accompanied with the expression of apoptosis-related proteins, Caspase-3, PARP, and Bax were up-regulated, whereas Bcl-2 protein expression was down-regulated. Interestingly, increased Caspase-3, PARP, and Bax with decreased Bcl-2 were observed in IPEC-J2 cells under heat stress conditions. In an in vivo porcine model, HS-induced cellular apoptosis in the duodenum, cecum, and colon, as well the up-regulation of HSP70 in the intestinal tissues.
Conclusions

HSP70 may play a regulatory role in cellular apoptosis within IPEC-J2 cells. Taken together, our findings provide new insights for understanding the molecular mechanisms of cellular apoptosis associated with HSP70.
The Global Fund: Anti-Corruption, Transparency and Accountability
Authors: Chang Zhihao , Kohler Jillian 
Published: December 2020

Abstract
Background: The Global Fund to Fight AIDS, Malaria and Tuberculosis has been a key international organization in improving the health of those affected by the three big diseases. It was created during a time of health crisis and did not have the necessary anti-corruption, transparency, and accountability (ACTA) structures in place to prevent fraud and corruption in its grants, which resulted in misuse of funds by grant recipients and loss of donor confidence in 2011. Almost one decade later, this paper seeks to describe the ACTA mechanisms within the Global Fund and their results. Results: At the highest level, the Board of Directors has restructured the Global Fund’s governing committees in 2011 and in 2016 to its current Audit and Finance, Ethics and Governance, and Strategy Committees. This has helped to delineate committee mandates and to strengthen the Board’s oversight and direction on operations. In addition, the Global Fund has adopted a rigorous risk management framework and has worked risk mitigation into all aspects of functioning. An Ethics and Integrity Framework was adopted in 2014 and an Ethics Office was established in 2016, resulting in increased conflict of interest disclosures and greater ethics considerations within the Global Fund. The Office of the Inspector General of the Global Fund has been effective in performing internal and external audits and investigations on fraud and corruption, suggesting changes to mitigate future risks, and implementing novel initiatives, such as the I Speak Out Now! campaign to encourage whistleblowing and to educate on signs of fraud and corruption. Finally, the “eyes and ears” of the Global Fund, the Local Fund Agents, have been involved in exposing fraud and corruption during the implementation of Global Fund grants. These mechanisms have reduced grant-related risks and procurement fraud in particular. Conclusions: Over the past decade, the Global Fund has developed a number of ACTA mechanisms. It will be critical that the Global Fund continues to monitor and evaluate how effective these mechanisms are and to make changes, when and where needed.
Health care workers depression and associated factors during COVID-19. Health facility-based study in Eastern Ethiopia
Authors: Yadeta Tesfaye Assebe , Baca Yadeta Desie , Balis Bikila 
Published: December 2020

Abstract
Background

Depression of health care workers was related to work absences, resignations, and poor work performance, affecting the quality of patient care and the health care system. It may be at higher risk during the Coronavirus disease (COVID-19) pandemic. There is limited evidence that assesses depression and its associated factors in the study area. Therefore this study aimed to assess depression and associated factors among health care workers (HCW) in eastern Ethiopia.
Method:

The cross-sectional study design was conducted from October 26th to November 10, 2020. To assess depression among health care workers. Patient Health Questionnaire (PHQ-19) questionnaire was used for the collection of depressive symptoms. The data were analyzed by using STATA version 14 software. Adjusted Odds Ratio (AOR) along with 95% CI was estimated to examine factors associated with depression symptoms using logistic regression analysis.
Results

Of the total 265 study participant, 176 (66.4%) and 95% CI: 60.4%, 71.8% of them reported depressive symptom. Of 176 reported symptoms of depression 27.9%, 24.1%, 9.4%, 3.7%, and 1.1% were had minimal, mild, moderate, moderate-severe, and severe depressive symptoms respectively. Being female [AOR: 3.40, (95%CI: (1.79, 6.45)], participants who perceived susceptibility to COVID-19 [AOR: 2.30, (95%CI: (1.12, 4.71)], and perceived severity to COVID-19 [AOR: 3.06, (95%CI: (1.35, 6.91)] were higher risks of experiencing depression symptoms. Participants who had work experience 3–5 years [AOR: 0.21, (95%CI: (0.08, 0.56)], and the participant who had 6–10 years’ work experience [AOR: 0.30, (95%CI: (0.11, 0. 84)] were had less likely to experience depression symptoms when compared with a participant who had two and fewer years of work experience.
Conclusions

A high proportion of health care workers experienced depression symptoms. Health care workers' mental health needs to be protected during the COVID-19 pandemic. Female health care workers, junior health care workers, and HCWs perceived susceptibility, and perceived severity of COVID-19 need attention.
Correlation between the coagulation test and neonatal hyperbilirubinemia
Authors: zhou fangye , Li Lei , Fu Rong 
Published: December 2020

Abstract
Background

The coagulation test is used to diagnose the hematological diseases. Little is known about correlation between the coagulation test and neonatal hyperbilirubinemia. The aim of the study was to describe neonatal coagulation test in infants with neonatal hyperbilirubinemia.
Methods

Data were collected from newborns who had neonatal hyperbilirubinemia and healthy in Chengdu Second People’s Hospital. Prothrombin time(PT), Thrombin time(TT), Fibrinogen(Fbg), activated partial thromboplastin time(APTT), and calculated international normalized ration(INR) values were recorded. Linear relationship between INR and level of total bilirubin and indirect bilirubin were analysed by linear regression. Comparisons of two groups were made by GraphPad Prism 8.
Results

In this case-control study, the mean PT, APTT level were significant higher in the infants with hyperbilirubinemia group compared to the healthy infants. We found the correlation between INR and total bilirubin(R = 0.3327; P < 0.0001). There was also correlation between INR and indirect bilirubin(R = 0.3402; P < 0.0001).
Conclusions

PT, APTT were significant high in neonatal hyperbilirubinemia group. Correlation were observed between the coagulation test and neonatal hyperbilirubinemia.
Characterisation of National Immunisation Programmes (NIPs) in the context of Public Health Emergencies (PHEs): A Case Study of 13 Countries in the WHO African Region
Authors: Chepkurui Viola , Amponsah-Dacosta Edina , Eposi Haddison Christiana , Kagina Benjamin Mugo 
Published: December 2020

Abstract
Background In the World Health Organisation African region (WHO-Afro), multiple public health emergencies (PHEs) are experienced annually. PHEs are known to affect the provision of health services including immunisation. To our knowledge, there is limited information on the characterisation of PHEs and the performance of national immunisation programmes (NIPs) in countries within the WHO-Afro. This study assessed PHEs (armed conflicts, disasters, and disease outbreaks) and the performance of NIPs within PHE contexts using global and regional immunisation targets.MethodsCountries recorded to have benefitted from PHE mitigation funds from the African Public Health Emergency Fund (APHEF) were used as case studies. Data on PHEs and immunisation indicators between 2010 and 2019 in the selected countries were extracted from different PHE electronic databases and the WHO/UNICEF immunisation database, respectively.The PHEs and immunisation indicators were stratified by country and summarised using descriptive statistics. The Mann-Whitney U test was carried out to determine the association between the frequency of PHEs and the performance of NIPs. Statistical significance was defined at p-value < 0.05. ResultsBetween 2010 and 2019 there were a total of 175 disease outbreaks, 288 armed conflicts, and 318 disasters in the 13 countries selected as case studies. The Democratic Republic of Congo had the highest total PHE count (n=208), while Liberia had the lowest (n=20). Only three of the 13 countries had a median coverage value for the third dose of the combined Diphtheria, Tetanus, and Pertussis vaccine (DTP3) that had attained the target for ≥90% immunisation coverage.Higher counts of armed conflict and total PHEs were associated with not meeting the immunisation targets for national DTP3 coverage of ≥90% and Maternal and Neonatal Tetanus (MNT) elimination, p<0.01. Higher disaster counts were also associated with not attaining MNT elimination, p=0.03.ConclusionPHEs are prevalent in the WHO-Afro, irrespective of the level of a country’s immunisation maturity. In absence of effective interventions, the PHEs have the potential to derail the progress of NIPs in the WHO-Afro. As we transition towards the Immunisation Agenda 2030, we recommend that the WHO-Afro prioritises interventions to mitigate the impacts of PHEs on the NIPs.
A Report of Two Cases of Bacteremia by Leuconostoc lactis in Immunocompromised Patients
Authors: Ge Yanmei , Ji Wenli , Hu Song , Tong Huacheng 
Published: December 2020

Abstract
Background Leuconostoc lactis (L lactis) are gram positive cocci and generally considered to be environmental organisms. They were widely regarded as non-pathogenic and therefore of limited clinical significance. Molecular identification was an important technique to guide antibiotic treatment.Case reports We presented two cases of bacteremia caused by L lactis. They were both advanced age, history of various diseases, intravenous catheter implantation, parenteral nutrition, longer hospital stay and using of multiple antibiotics. The 16S rRNA genes of the isolates of these two patients´ blood were sequenced. Conclusions When immunocompromised patients with risk factors develop bacteremia caused by L lactis, adequate attention and effective treatment measures should given timely.
LncRNA SNHG16 Facilitates Liver Metastases of Colorectal Cancer by Modulating Circulating Tumor Cells (CTCs) Epithelial-Mesenchymal Transition (EMT) via a Positive Feedback Loop with YAP1/TEAD1 Complex
Authors: Xiang Zhenxian , Huang Guoquan , Wu Haitao , He Qiuming , Yang Chaogang , Dou Rongzhang , Liu Qing , Song Jialing , Fang Yan , Wang Shuyi , Xiong bin 
Published: December 2020

Abstract
Background: Circulating tumor cells are important precursor of colorectal cancer metastasis, which attributes to the main cause of cancer-related death. The ability to adopt epithelial-mesenchymal transition (EMT) process facilitates CTCs generation, thereby overcoming metastatic bottlenecks and realizing distant metastasis. However, the potential molecular mechanism of CRC EMT remains largely unknown.Methods: RT-qPCR, immunohistochemical staining, and western blot were used to detect the expression of mRNA and protein in CRC. Loss- and gain-of-function approaches were performed to investigate the effect of SNHG16 on CRC cell phenotypes. Function assays, including wounding healing, transwell assay, and clone formation were used to assess the effect of SNHG16 on tumor biological behavior. Then, RNA immunoprecipitation, Chromatin Immunoprecipitation, Co-Immunoprecipitation, GST-pull down, biotin-labeled miR-195-5p pull down, and dual-luciferase assay were performed to uncover the underlying mechanism for molecular interaction. Finally, CRC nude mice xenograft model experiment was performed to evaluate the influence of SNHG16 on tumor progression in vivo Results: Compared with normal tissue and cell line, SNHG16 was significantly upregulated in CRC. Clinical investigation revealed that SNHG16 high expression was correlated with advanced TNM stage, distant metastasis, and poor prognosis of cancer patients. According to Loss- and gain-of-function experiment, SNHG16 could promote CRC proliferation, migration, invasion, EMT, mesenchymal-type CTCs (MCTCs) generation, and liver metastasis through YAP1 in vitro and in vivo. Mechanistic research indicates that, SNHG16 could act as miRNA sponge to sequester miR-195-5p on Ago2, thereby protecting YAP1 from repression and facilitating CRC liver metastasis and tumor progression. Moreover, YAP1 could combine with TEA Domain Transcription Factor 1 (TEAD1) to form a YAP1/TEAD1 complex, which could in turn bind to the promoter of SNHG16 and regulate its transcription. In addition, both of YAP1 and TEAD1 are indispensable during this process. Finally, we demonstrated that YAP1 significantly promoted the tumor progression, and SNHG16 could rescue the effect of YAP1 on tumor progressionConclusion: Herein, we clarified a hitherto unexplored positive feedback loop between SNHG16 and YAP1/TEAD1. These findings provided new sights in CRC liver metastasis, and it may act as a potential candidate in the treatment of CRC.
The Impact of Altered Metabolism on the Regulation of IGF-II/H19 Imprinting Status in Prostate Cancer.
Authors: Kingshott Georgina , Biernacka Kalina , Sewell Alex , Gwiti Paida , Barker Rachel , Zielinska Hanna , Gilkes Amanda , McCarthy Kathryn , Martin Richard M , Lane J Athene , McGeagh Lucy , Koupparis Anthony , Rowe Edward , Oxley Jon , Holly Jeff , Perks Claire M 
Published: December 2020

Abstract
Background: Prostate cancer is the most frequently diagnosed cancer type and the second major cause of cancer deaths amongst men. A link exists between obesity, type 2 diabetes, and cancer risk. Insulin-like growth factor II (IGF-II) plays a role in numerous cellular events, including proliferation and survival. The IGF-II gene shares its locus with the lncRNA, H19. IGF-II/H19 was also the first gene to be identified as being ‘imprinted’ – where the paternal copy is not transcribed. This silencing phenomenon is lost in many cancer types. Methods: We disrupted imprinting behaviour in vitro through the alteration of metabolic conditions and quantified it using RFLP, qPCR and pyrosequencing; changes to peptide were measured using RIA. Prostate tissue samples were analysed using ddPCR, pyrosequencing and IHC. We then compared with in silico data, provided by TGCA on the cBIO Portal.Results: Disruption of imprinting behaviour, in vitro, occurs at the molecular level with no changes to peptide. In vivo, most specimens primarily retained imprinting status, apart from a small subset which showed reduced imprinting. A positive correlation was seen between IGF-II and H19 mRNA expression, which concurred with findings of larger Cancer Genome Atlas (TGCA) cohorts. This positive correlation did not affect IGF-II peptide. Conclusions: Type 2 diabetes and / or obesity directly affect regulation growth factors involved in carcinogenesis.Trial registration: Prostate Cancer Evidence of Exercise and Nutrition Trial: nutritional and physical activity interventions for men with localised prostate cancer – feasibility study (ISRCTN99048944). Registered on 17 November 2014