bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plant cell cultures as food—aspects of sustainability and safety
Authors: Häkkinen Suvi T. , Nygren Heli , Nohynek Liisa , Puupponen-Pimiä Riitta , Heiniö Raija-Liisa , Maiorova Natalia , Rischer Heiko , Ritala Anneli 
Published: September 2020

Abstract
Key message
Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.

Abstract
Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agriculturally derived commodities. Plant cell cultures used for food could supplement current food production. However, still many aspects need to be resolved before this new food concept can enter the market. Issues related to sustainability and safety for human consumption are relevant for both consumers and regulators. In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose. Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively. Furthermore, nutritional composition or sensory properties were not compromised. Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles. Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
Leaving and staying with the employer—Changes in work, health, and work ability among older workers
Authors: Garthe Nina , Hasselhorn Hans Martin 
Published: September 2020

AbstractObjectiveThe aim of this prospective study was to examine employer changes among older workers and to relate them to psychosocial work factors, health, and work ability. Four groups of employees as elaborated by Hom et al. (2012) were distinguished: Enthusiastic leavers (EL), reluctant leavers (RL), enthusiastic stayers (ES), and reluctant stayers (RS).MethodsRepeated Measures ANOVA analyses were based on data from the second and third waves (2014, 2018) of the German lidA Cohort Study, a representative study of employees born in 1959 or 1965.ResultsThe largest proportion of participants was ES (73.3%), 13.2% stayed with their employer although they would have preferred to leave (RS). 7.1% changed employer between 2014 and 2018 voluntarily (EL), 6.4% involuntarily (RL). Analyses confirmed that the four groups already differed in 2014 in terms of health, work ability, and psychosocial work factors and that these outcomes change in different characteristic patterns over time. Most outcomes improved substantially following the change among EL. RS already reported poor outcomes in 2014 and exhibited a further deterioration while staying at the undesired workplace.ConclusionOur findings indicate that an employer change is followed by improvements of work, health, and work ability. We conclude that an inclusive labor market policy for older workers allowing for high job mobility may have the potential to contribute to considerable improvements of workers’ individual working conditions, health, and work ability, thereby increasing the work participation. Also, the considerable group of RL requires increased political and scientific attention.
Basic psychological need satisfaction across the retirement transition: Changes and longitudinal associations with depressive symptoms
Authors: Stenling Andreas , Henning Georg , Bjälkebring Pär , Tafvelin Susanne , Kivi Marie , Johansson Boo , Lindwall Magnus 
Published: September 2020

AbstractDrawing on self-determination theory, the present study examined how satisfaction of the basic psychological needs (autonomy, competence, and relatedness) changes across the retirement transition and how need satisfaction was related to depressive symptoms across the retirement transition. Participants (N = 2655) were drawn from the HEalth, Ageing and Retirement Transitions in Sweden (HEARTS) study. Latent growth curve modeling showed that autonomy need satisfaction increased across the retirement transition, whereas competence and relatedness remained relatively stable. Higher need satisfaction was related to less depressive symptoms at baseline, however, pre-retirement need satisfaction was not a statistically significant predictor of subsequent changes in depressive symptoms (or vice versa) across the retirement transition. At the within-person level, higher than usual need satisfaction at a specific time point was related to less than usual depressive symptoms. Need satisfaction may be an important factor to consider across the retirement transition and need satisfying activities prior, during, and after the transition may ease peoples’ adjustment to retirement.
Acacetin improves endothelial dysfunction and aortic fibrosis in insulin-resistant SHR rats by estrogen receptors
Authors: Wei Yaxin , Yuan Peipei , Zhang Qi , Fu Yang , Hou Ying , Gao Liyuan , Zheng Xiaoke , Feng Weisheng 
Published: September 2020

AbstractThe aim of the work was to investigate the effects of acacetin on endothelial dysfunction and aortic fibrosis in insulin-resistant SHR rats and explore its mechanism. Seven-week-old male spontaneously hypertensive rats (SHR) were selected to establish a rat model of hypertension with insulin resistance induced by 10% fructose. The nuclear factor kappa B p65 (NF-κB p65) and Collagen I were observed by Immunohistochemistry. Immunofluorescence was used to observe estrogen receptor-alpha (ERα), estrogen receptor-beta (ERβ), and G protein-coupled receptor 30 (GPR30). Western blotting was used to detect interleukin (IL-1β), Arginase 2 (ARG2), Nostrin, endothelial nitric oxide synthase (eNOS), TGF-β, Smad3, ERK pathway proteins such as p-c-Raf, p-MEK1/2, p-ERK, ERK, p-P90RSK and p-MSK1. We found that acacetin did have an improvement on endothelial dysfunction and fibrosis. Meanwhile, it was also found to have a significant effect on the level of estrogen in this model by accident. Then, the experiment of uterine weight gain in mice confirmed that acacetin had a certain estrogen-like effect in vivo and played its role through the estrogen receptors pathway. In vitro experience HUVEC cells were stimulated with 30 mM/L glucose and 100 mM/L NaCl for 24 h to establish the endothelial cell injury model. HUVEC cells were treated with 1 μM/L estrogen receptors antagonist (ICI 182780) for 30 min before administration. Cell experiments showed that acacetin could reduce the apoptosis of HUVEC cells, the levels of inflammatory cytokines and the expression of TGF-β, Collagen I and Smad3 in endothelial cell injury model. After treatment with ICI 182780, the improvement of acacetin was significantly reversed. The results showed that acacetin relieved endothelial dysfunction and reduced the aortic fibrosis in insulin-resistant SHR rats by reducing the release of inflammatory factors and improving vasodilatory function through estrogen signaling pathway.
Human segmentation in surveillance video with deep learning
Authors: Gruosso Monica , Capece Nicola , Erra Ugo 
Published: September 2020

AbstractAdvanced intelligent surveillance systems are able to automatically analyze video of surveillance data without human intervention. These systems allow high accuracy of human activity recognition and then a high-level activity evaluation. To provide such features, an intelligent surveillance system requires a background subtraction scheme for human segmentation that captures a sequence of images containing moving humans from the reference background image. This paper proposes an alternative approach for human segmentation in videos through the use of a deep convolutional neural network. Two specific datasets were created to train our network, using the shapes of 35 different moving actors arranged on background images related to the area where the camera is located, allowing the network to take advantage of the entire site chosen for video surveillance. To assess the proposed approach, we compare our results with an Adobe Photoshop tool called Select Subject, the conditional generative adversarial network Pix2Pix, and the fully-convolutional model for real-time instance segmentation Yolact. The results show that the main benefit of our method is the possibility to automatically recognize and segment people in videos without constraints on camera and people movements in the scene (Video, code and datasets are available at http://graphics.unibas.it/www/HumanSegmentation/index.md.html).
The role of the comprehensive complication index for the prediction of survival after liver transplantation
Authors: Lai Quirino , Melandro Fabio , Nowak Greg , Nicolini Daniele , Iesari Samuele , Fasolo Elisa , Mennini Gianluca , Romano Antonio , Mocchegiani Federico , Ackenine Kevin , Polacco Marina , Marinelli Laura , Ciccarelli Olga , Zanus Giacomo , Vivarelli Marco , Cillo Umberto , Rossi Massimo , Ericzon Bo-Göran , Lerut Jan 
Published: September 2020

AbstractIn the last years, several scoring systems based on pre- and post-transplant parameters have been developed to predict early post-LT graft function. However, some of them showed poor diagnostic abilities. This study aims to evaluate the role of the comprehensive complication index (CCI) as a useful scoring system for accurately predicting 90-day and 1-year graft loss after liver transplantation. A training set (n = 1262) and a validation set (n = 520) were obtained. The study was registered at https://www.ClinicalTrials.gov (ID: NCT03723317). CCI exhibited the best diagnostic performance for 90 days in the training (AUC = 0.94; p < 0.001) and Validation Sets (AUC = 0.77; p < 0.001) when compared to the BAR, D-MELD, MELD, and EAD scores. The cut-off value of 47.3 (third quartile) showed a diagnostic odds ratio of 48.3 and 7.0 in the two sets, respectively. As for 1-year graft loss, CCI showed good performances in the training (AUC = 0.88; p < 0.001) and validation sets (AUC = 0.75; p < 0.001). The threshold of 47.3 showed a diagnostic odds ratio of 21.0 and 5.4 in the two sets, respectively. All the other tested scores always showed AUCs < 0.70 in both the sets. CCI showed a good stratification ability in terms of graft loss rates in both the sets (log-rank p < 0.001). In the patients exceeding the CCI ninth decile, 1-year graft survival rates were only 0.7% and 23.1% in training and validation sets, respectively. CCI shows a very good diagnostic power for 90-day and 1-year graft loss in different sets of patients, indicating better accuracy with respect to other pre- and post-LT scores.Clinical Trial Notification: NCT03723317.
Confronting hidden COVID-19 burden: a telemedical solution for elective urological outpatient clinics
Authors: Westhofen Thilo , Magistro Giuseppe , Lennartz Simon , Casuscelli Jozefina , Stief Christian , Rodler Severin 
Published: September 2020

AbstractMaintaining high-quality care for urological patients is a challenge during and after the Coronavirus disease 2019 (COVID-19) pandemic. We observe an increasing volume of postponed elective visits at our tertiary care hospital, holding the risk for deterioration of non-emergency disease conditions. As it is unclear for how long the pandemic will last, we propose to implement telehealth as a solution to provide regular symptom monitoring compatible with social distancing guidelines during the pandemic and beyond. Telemedical assessment and prioritizing of high-risk patients for individual consults at outpatient services will have to be aligned with available outpatient capacity and local outbreak severity.
Breakage behavior of biomass pellets: an experimental and numerical study
Authors: Gilvari Hamid , de Jong Wiebren , Schott Dingena L. 
Published: September 2020

AbstractThe presence and generation of fines and dust in the bulk of biomass pellets have inflicted several problems in the supply chain during transportation and storage, and the breakage behavior of pellets has been scarcely studied so far. Fines and dust are the consequences of impact and abrasive forces through the whole supply chain; however, the breakage happens at the particle level. Therefore, to study the fines generation, first, the breakage behavior of individual pellets should be understood, and then, the behavior of the bulk materials in operational conditions can be investigated. This paper aims to investigate the breakage behavior of individual pellets under experimental compression tests and to introduce a calibrated numerical model using discrete element method (DEM) in order to pave the way for further studies on pellet breakage. For that purpose, seven different types of biomass pellets were studied experimentally, and then, a calibrated model was introduced via the Timoshenko–Ehrenfest beam theory using DEM. Results show that the model could reasonably predict the breakage behavior of pellets under uniaxial and diametrical compressions. The findings could help to develop a new design of the equipment for transportation and handling of biomass pellets with the aim to reduce the amount of generating fines and dust.
Cardiometabolic health, visceral fat and circulating irisin levels: results from a real-world weight loss study
Authors: Miazgowski T. , Kaczmarkiewicz A. , Miazgowski B. , Kopeć J. 
Published: September 2020

AbstractBackgroundThe aim of this pragmatic intervention study was to investigate changes in cardiometabolic outcomes, irisin plasma concentration, and body composition during a 4-month intervention in unselected obese individuals.Materials and methodsIn 111 obese women aged 36.73 ± 7.2 years, we measured changes in weight, lipid profiles, glucose, insulin, Homeostatic Model Assessment-Insulin Resistance Index (HOMA-IR), uric acid, aminotransferases, and irisin. Body composition including lean mass (LM) and total (TF), gynoid (GF), android (AF), and visceral fat (VF) was assessed using densitometry. Physical activity was assessed using the International Physical Activity Questionnaire (IPAQ). The participants received tailored written advice targeting lifestyle according to current guidelines. At follow-up, patients rated their adherence in the self-administered questionnaire.ResultsMean weight loss in the whole group was 3.12 kg (− 3.3%); 26% of the women achieved the desired target of weight loss (> 5% of the initial weight), whereas weight decreased moderately in 50% and increased in 14%. In 86 women with weight loss, there were significant changes in HOMA-IR (− 13.8%), insulin (− 11.2%), alanine aminotransferase (− 8.0%), VF (− 7.0%), AF (− 5.4%), TF (− 4.7%), GF (− 2.8%) and LM (− 1.5%), whereas irisin and HDL-C levels and the mean IPAQ score did not change.ConclusionsIn this real-world evidence study, a successful weight loss achieved only 26% of patients, with overall much better adherence to diet restriction than to exercise. However, even mild to moderate weight loss resulted in significant improvements in cardiometabolic health. Weight loss was associated with a modest LM decrease but did not influence plasma irisin.
A new theory on the cause of anal fissure – impaction theory
Authors: Chen Shaoming , Yu Qinghuan 
Published: September 2020

AbstractThe internal hemorrhoid, rectal tumor, hypertrophic anal papilla, and secret fecal mass are regarded as the blocks embedded in the rectum. The above blocks hinder defecation, which will inevitably lead to excessive opening of the anal caliber. Once the limit is exceeded, the skin of the anal canal will tear and form anal fissure. Based on the study of historical evolution, hypothesis reasoning, clinical verification and comparison with other theories, a new concept of anal fissure etiology–impaction theory is proposed. The so-called impaction theory refers to the impaction (various primary lesions) in anorectum, which hinders defecation. When defecating, the anal canal expands beyond the limit, and the whole layer of anal canal skin splits, that is to say, anal fissure is formed.
The Vulvodynia Experience Questionnaire: Qualitative Development of a New Patient-Reported Outcome Measure for Vulvodynia
Authors: Goldstein Andrew T. , Diez Pedro M. Quintana , Kapanadze Sofia , Cala Mary Lynn , Evans Christopher J. , Whyte Julie L. , Christoph Annette 
Published: September 2020

Abstract

Introduction
Vulvodynia is defined as vulvar pain of at least 3 months duration without a clear identifiable cause. There are currently no validated questionnaires that assess the experience of women with localized vulvodynia of the vestibule (vestibulodynia, previously known as vulvar vestibulitis) that meet the requirements of the Food and Drug Administration's Patient Reported Outcome (PRO) Guidance.

Aim
To develop a new content-valid PRO assessment in accordance with the Food and Drug Administration's PRO guidance to assess the symptoms and impacts of localized vulvodynia.

Material and Methods
Participants were recruited for concept elicitation interviews (ie, interviews with open-ended questions with the goal of eliciting volunteered data about the symptoms and impacts of vulvodynia). Participants were identified as having localized vulvodynia by clinicians who were experts in treating vulvar disorders. Eligibility was confirmed by the recruiting clinician, and informed consent was obtained; participants were then scheduled for in-person interviews. 25 participants were interviewed from United States (US). After concept elicitation interviews, the draft Vulvodynia Experience Questionnaire (VEQ) was developed based on the results. Cognitive interviews were conducted with 20 participants from US sites to assess the content validity of the VEQ (eg, interpretation and clarity of the items, relevance of concepts). The VEQ was further revised after cognitive interviews. All interviews were conducted face-to-face, audio-recorded, transcribed verbatim, anonymized, and analyzed using a qualitative data analysis software program.

Results
17 unique symptoms and 32 unique impacts were reported during concept elicitation interviews. Pain (n = 25, 100%) and burning (n = 24, 96%) were the most frequently reported symptoms of localized vulvodynia, and negative impact on emotional well-being (n = 25, 100%) was the most frequently reported impact. After analysis, item generation, and cognitive interviews, the resulting VEQ v2.0 contains 3 parts (part 1, pain; part 2, associated symptoms; part 3, impacts) with a total of 25 items that measure the most frequently reported symptoms and impacts of localized vulvodynia.

Strength and Limitations
The VEQ is a multidimensional assessment of the core symptoms and impacts of localized vulvodynia that, after additional psychometric testing including the ability to detect change, may be used in clinical trials to characterize the benefits of novel treatments. The VEQ requires additional testing to establish its cultural relevance and linguistic validity in other countries.

Conclusion
The VEQ is a novel method of collecting information on localized vulvodynia symptoms and impacts that may be suitable for use in clinical trials after psychometric testing.
AlN MEMS filters with extremely high bandwidth widening capability
Authors: Gao Anming , Liu Kangfu , Liang Junrui , Wu Tao 
Published: September 2020

AbstractThis paper presents radio frequency (RF) microelectromechanical system (MEMS) filters with extremely high bandwidth widening capability. The proposed filtering topologies include hybrid configurations consisting of piezoelectric MEMS resonators and surface-mounted lumped elements. The MEMS resonators set the center frequency and provide electromechanical coupling to construct the filters, while the lumped-element-based matching networks help widen the bandwidth (BW) and enhance the out-of-band rejection. Aluminum nitride (AlN) S0 Lamb wave resonators are then applied to the proposed filtering topologies. AlN S0 first- and second-order wideband filters are studied and have shown prominent performance. Finally, the AlN S0 first-order wideband filter is experimentally implemented and characterized. The demonstrated first-order filter shows a large fractional bandwidth (FBW) of 5.6% (achieved with a resonator coupling of 0.94%) and a low insertion loss (IL) of 1.84 dB. The extracted bandwidth widening factor (BWF) is 6, which is approximately 12 times higher than those of the current ladder or lattice filtering topologies. This impressive bandwidth widening capability holds great potential for satisfying the stringent BW requirements of bands n77, n78, and n79 of 5G new radio (NR) and will overcome an outstanding technology hurdle in placing 5G NR into the marketplace.
Electrochemical measurement of serotonin by Au-CNT electrodes fabricated on microporous cell culture membranes
Authors: Chapin Ashley A. , Rajasekaran Pradeep R. , Quan David N. , Hu Liangbing , Herberholz Jens , Bentley William E. , Ghodssi Reza 
Published: September 2020

AbstractGut–brain axis (GBA) communication relies on serotonin (5-HT) signaling between the gut epithelium and the peripheral nervous system, where 5-HT release patterns from the basolateral (i.e., bottom) side of the epithelium activate nerve afferents. There have been few quantitative studies of this gut-neuron signaling due to a lack of real-time measurement tools that can access the basolateral gut epithelium. In vitro platforms allow quantitative studies of cultured gut tissue, but they mainly employ offline and endpoint assays that cannot resolve dynamic molecular-release patterns. Here, we present the modification of a microporous cell culture membrane with carbon nanotube-coated gold (Au-CNT) electrodes capable of continuous, label-free, and direct detection of 5-HT at physiological concentrations. Electrochemical characterization of single-walled carbon nanotube (SWCNT)-coated Au electrodes shows increased electroactive surface area, 5-HT specificity, sensitivity, and saturation time, which are correlated with the CNT film drop-cast volume. Two microliters of CNT films, with a 10-min saturation time, 0.6 μA/μM 5-HT sensitivity, and reliable detection within a linear range of 500 nM–10 μM 5-HT, can be targeted for high-concentration, high-time-resolution 5-HT monitoring. CNT films (12.5 μL) with a 2-h saturation time, 4.5 μA/μM 5-HT sensitivity, and quantitative detection in the linear range of 100 nM–1 μM can target low concentrations with low time resolution. These electrodes achieved continuous detection of dynamic diffusion across the porous membrane, mimicking basolateral 5-HT release from cells, and detection of cell-released 5-HT from separately cultured RIN14B cell supernatant. Electrode-integrated cell culture systems such as this can improve in vitro molecular detection mechanisms and aid in quantitative GBA signaling studies.