bluuebunny/crossref_metadata_2025_split huggingface dataset:
Double plating with autogenous bone grafting as a salvage procedure for recalcitrant humeral shaft nonunion
Authors: Feng Dongxu , Wang Xiaolong , Sun Liang , Cai Xiao , Zhang Kun , Wang Zhan , Zhu Yangjun 
Published: August 2020

Abstract
Background: Despite the majority of humeral shaft nonunions respond well to surgical intervention, a surgeon still encounters a patient with humeral shaft nonunion who had already undergone repeated surgeries for nonunion. This study is a retrospective analysis of the efficacy of the treatment of recalcitrant humeral shaft nonunions using double locking compression plate (LCP) fixation in combination with autogenous iliac crest bone grafting.Methods: A consecutive series of aseptic recalcitrant humeral shaft nonunion underwent surgical treatment between May 2010 and August 2017 in the authors’ institute. Standardized treatment included a thorough debridement, double LCP and screw fixation, and autogenous iliac bone graft. The injury type, the bone affected by nonunion, and the duration of nonunion were recorded for all patients. The main outcome measurements were Constant and Murley scale for shoulder function, Mayo elbow performance index (MEPI) for elbow function, and the visual analog scale (VAS) for pain. In addition, all complications were documented.Results: The study consisted of six females and nine males with a mean age of 45.3±13.1 years. Each patient had already undergone at least once failed surgical management for nonunion. The average duration that the bone remained ununited before the index intervention was 126.8±124.2 months. All patients achieved bone union without implant failure. At the final follow-up, both the mean Constant and Murley joint function score and the mean MEPI were significantly improved, and the mean VAS score significantly decreased. Each patient was highly satisfied with the treatment. Complications were only seen in four patents, including one super wound infection, one radial nerve palsy, one ulnar never palsy, and one discomfort at the iliac crest.Conclusion: Double plate fixation combined with autogenous iliac crest bone grafting can result in successful salvage of humerus nonunions in patients who have failed prior surgical interventions.
Effect of Acupotomy in Knee Osteoarthritis Patients: Study Protocol for A Randomized Controlled Trial
Authors: Xu Danghan , Lee Minghui , Huang Cihui , Wei Jia , Zhou Mengxue , Yao Taotao , Lu Jingjing , Zhao Wenjing , Xu Nuo , Huang Ruina , He Jun , Zheng Liang 
Published: August 2020

Abstract
BackgroundSymptomatic knee osteoarthritis patients are common in China. Pharmacologic therapy is not the first recommendation because of its safety issues. Non-pharmacologic therapy, such as lifestyle adjustments, weight loss, and proper exercise is strongly recommended to use in knee osteoarthritis. But the adherance rate is very poor. Acupotomy therapy, as an effective treatment for knee osteoarthritis, is lack of high quality randomized controlled trials. This study set out to investigate the efficacy of acupotomy in patients with knee osteoarthritis.Methods136 patients will be enrolled in the First Affiliated Hospital of Guangzhou University of Chinese Medicine, and assigned into acupotomy group or sham acupotomy group according to the block randomization number. The acupotomy group will receive 2 sessions of acupotomy for 2 weeks(once a week). The sham group will receive 2 sessions of sham stimulation for 2 weeks༈once 2 week. All the patients will use indomethacin cream externally. The primary outcome is the WOMAC index and the secondary outcomes are VAS, plantar pressure distribution test, X-ray examination, musculoskeletal ultrasound, maximum knee circumference, and joint mobility. We will measure them at baseline, one week after the end of treatments, the third month and sixth month follow up.DiscussionAs we know, this is the first single-blinding and sham controlled design in acupotomy research. We aim to prove the efficacy of acupotomy in treating knee osteoarthritis.Trial registrationChinese Clinical Trial Registry: ChiCTR2000033047(2020-5-18).
A genome-wide screening for RNAi pathway proteins in Acari
Authors: Nganso Beatrice T , Sela Noa , Soroker Victoria 
Published: August 2020

Abstract
Background RNA interference (RNAi) is a highly conserved, sequence-specific gene silencing mechanism present in Eukaryotes. Three RNAi pathways critical for organismal development and survival are known, namely micro-RNA (miRNA), Piwi-interacting RNA (piRNA) and short interfering RNA (siRNA) pathways. Little knowledge exist about the genes involved in these pathways in Acari. Moreover, variable successes has been obtained in gene knockdown via siRNA pathway in functional genomics and management of Acari species. We hypothesized that the clue may be in the variability in the composition and the efficacy of siRNAi machinery among Acari. Results Both comparative genomic analyses and domain annotation suggest that all the analyzed species have orthologs of genes that mediate gene silencing via the three RNAi pathways though gene duplication and/or loss have occurred in the different species. We also identified orthologs of Caenorhabditis elegans RNA-dependent RNA polymerase (RdRP) gene in all Acari species though no secondary Argonaute homologs that operate with this gene in siRNA amplification mechanism were found. This finding suggests that the siRNA amplification mechanism present in Acari may be distinct from that described in C. elegans . Moreover, the genomes of these Acari species encode no ortholog of C. elegans systemic RNAi defective 1 (Sid-1) that mediate systemic RNAi, suggesting that the phenomena of systemic and parental RNAi that has been reported in some Acari species probably occur through a different mechanism. Orthologs of RNAi spreading defective-3 (Rsd-3) gene and scavenger receptors namely Eater and SR-CI that mediate endocytosis cellular update of dsRNA in C. elegans and Drosophila melanogaster were found in Acari genomes. This result suggests that cellular dsRNA uptake in Acari is endocytosis-dependent. Detailed phylogenetic analyses of core RNAi pathway genes in the studied Acari species revealed that their evolution is compatible with the proposed monophyletic evolution of this group. Conclusions Taken together, our in silico comparative analyses have revealed the potential activity of all three pathways in Acari. However, much experimental work remains to be done in elucidating the operating mechanisms behind these pathways in particular those that govern systemic/parental RNAi and siRNA amplification in Acari.
Recombinant human bone morphogenetic protein -2 in combination with&nbsp;β-Tricalcium Phosphate and&nbsp;Platelet Rich Fibrin for Socket Preservation before Implant Placement
Authors: Durge Khushboo Jeevan , Baliga Vidya Sudhindra , Srid Shilpa Banglore , Dhadse Prasad Vijayrao , Ragit Gayatri Chandrakant 
Published: August 2020

Abstract
Objectives –Periosteum is a rich source of osteoprogenitor cells, and flapless extraction technique allows for preservation of periosteum. Recombinant Bone Morphogenetic Protein-2 has osteoinductive capacity and has been exploited for bone augmentation. The aim of this study is to determine the effectiveness of rhBMP-2 for socket preservation along with β-TCP and PRF to enhance bone quality, clinically and radiographically.Results- Minimal crestal bone changes were seen at 3 months compared to baseline values; bone loss of 0.6 ± 0.13 mm at mesio-distal aspect, 0.5 ± 0.13 mm at bucco-lingual aspect. At 3 months reentry, no residual bone particulate graft were seen in any of site. Clinical hardness of bone evaluated through tactile perception while drilling, prior to implant placement, was analogous to drilling into spruce or white pine wood. Histologic analysis confirmed the absence of residual bone graft indicating new bone formation .Complete radiographic fill was seen at 3 months. The patients in current study showed adequate bone formation in grafted sockets and required no additional augmentation during implant placement. Therefore, based on the results and within the limitations of study it can be suggested that, rhBMP-2 with PRF and β-TCP can be recommended for socket preservation prior to implant placement
Transcription Factor CTCFL Promotes cell Proliferation, Migration and Invasion in Gastric Cancer Via Activating DPPA2
Authors: Yao Haibo , Shao Qinshu , Shao Yanfei 
Published: August 2020

Abstract
Background: The purpose of this study was to explore the relationship between CTCFL and DPPA2, and validate the positive role of CTCFL/DPPA2 in cell proliferation, migration and invasion in gastric cancer.Methods: Bioinformatics methods were applied for the prediction of gastric cancer-related transcription factors and corresponding target mRNAs. qRT-PCR and western blot were performed to test the levels of CTCFL and DPPA2. Then a series of in vitro experiments were conducted to assay the cell biological behaviors, including CCK-8, colony formation assay, wound healing assay and Transwell invasion assay. CHIP was carried out for assessment of the targeted relationship between CTCFL and DPPA2.Results: CTCFL and DPPA2 were both highly expressed in gastric cancer cells, and high CTCFLL and DPPA2 could promote cell proliferation, migration and invasion. CHIP validated that DPPA2 was a target of CTCFL. In addition, high DPPA2 could reverse the inhibitory effect of CTCFL silencing on the cell proliferation, migration and invasion in gastric cancer.Conclusion: The transcription factor CTCFL promotes cell proliferation, migration and invasion in gastric cancer via activating DPPA2.
miR-145-5p Overexpression Inhibits the Proliferation, Migration and Invasion of Esophageal Carcinoma Cells by Targeting ABRACL
Authors: Fan Shengming , Chen Pei , Li Shugang 
Published: August 2020

Abstract
Background: The study aimed to investigate the regulatory relationship between miR-145-5p and ABRACL, and tried to clarify the mechanisms underlying the proliferation, migration and invasion of esophageal cancer (EC) cells.Methods: Gene expression data related to EC were accessed from TCGA database and the “edgeR” package was used to screen the differentially expressed miRNA (DEmiRNAs) and genes (DEGs). TargetScan, miRDB and miRTarBase databases were used to predict potential targets for the target miRNA miR-145-5p. qRT-PCR and Western blot were performed to assess the expression of miR-145-5p and ABRACL in EC cells. Dual-luciferase reporter assay was performed for verification of the targeting relationship between miR-145-5p and ABRACL. Functional experiments including colony formation assay, Transwell migration and invasion assays were used to detect the proliferation, migration and invasion abilities of EC cells. Results: The expression of miR-145-5p was significantly decreased in EC, while ABRACL was remarkably increased. In addition, there was a negative correlation identified between miR-145-5p and ABRACL expression levels. Overexpressing miR-145-5p was able to suppress cell proliferation, migration and invasion, whereas silencing miR-145-5p posed an opposite effect. In the meantime, ABRACL was identified as a direct target of miR-145-5p by dual-luciferase reporter assay. Furthermore, miR-145-5p could inhibit the expression of ABRACL, in turn inhibiting the proliferation, migration and invasion of EC cells. Conclusion: miR-145-5p functions on the proliferation, migration and invasion of EC cells via targeting ABRACL, and it may be a novel therapeutic target for EC treatment.
The monocyte-to-lymphocyte ratio and depression in diabetes patients
Authors: Zhou Depu , Wang Jie , Li Xiaokun 
Published: August 2020

Abstract
Background: The purpose of this study was to determine the association between The monocyte-to-lymphocyte ratio (MLR) and depression with diabetes mellitus.Method: We examined data from the US National Health and Nutrition Examination Survey from 2009 to 2016. Cox proportional hazard models were used to calculate the associations between MLR and depression. For precise investigation of the relationship, we also plotted the smooth curve fit and generated a two-piecewise linear regression model using the penalized spline method.Result: We enrolled 2820 diabetes patients in the database. Diabetes patients who had high MLR tended to be young, female, obese, unmarried, and had low levels of education. For tertile analysis, the ORs and 95% CIs of clinically relevant depression in tertile analysis were 1.03 (0.91, 1.17) for the second group and 1.62 (1.44, 1.82) for the third group in the unadjusted model compared to the control group. A similar trend was observed for the adjusted model and the quartiles analysis. We found the inflection point of MLR was 2.7. There is a positive association between MLR and depression above the threshold, and no relationship was found when MLR was below the threshold in diabetes patients. Conclusion: There is a nonlinear relationship between MLR and depression in diabetes patients. High level of MLR more than the inflection point may add prognostic information for depression in diabetes patients.
Riverbank Erosion and Migration, Inter-linkage: With Special Focus on Assam, India
Authors: Dekaraja Dimpal , Mahanta Ratul 
Published: August 2020

Abstract
Background: River-bank erosion becomes a vulnerable phenomenon in the bank of the Brahmaputra river and its tributaries. Around 17 riverine districts of Assam are affected by riverbank erosion and lost large plots of land. Due to riverbank erosion the victims’ loss their homestead and crop land as well as their survival strategy in the eroded areas. Moreover, farmers largely affected due to riverbank erosion as they loss their sources of income. This forces the farmer to migrate to another place for their survival. The paper examines the linkage between river bank erosion and migration based on secondary information. To examine the linkage, information collected from government published source ‘Census of India’ and ‘Statistical handbook of Assam’. On the basis of the information, 10 indicators constructed from 26 variables and then factor analysis method applied to examine the linkage between riverbank erosion and migration. Results: Four variables that are agricultural worker, industrial worker, cropped area and livestock population are loaded into the first factor, for which the first factor is labeled as the socio-economic indicator. In case of second factor the two variables i.e. MGI and urban population are loaded. On the basis of the loaded variables this factor labeled as demographic indicator. In case of third factor also two variables are loaded that is, NGI (Natural Growth Index) and Literacy rate. The loaded variables indicate that both NGI and Literacy rate are positively related. Two variables are loaded in this fourth factor i.e. river bank eroded area and the district population growth. This factor labeled as environmental indicator on the basis of the variables loaded in this factor. The factor correlation matrix indicate the opposite relation between first and fourth factor.Conclusion: The results obtained from Factor component analysis reveals that the first and fourth factor component mainly established the linkage between riverbank erosion and migration. Besides this the component correlation matrix also reveals the inter-linkage between the variables. Thus we can say that there is positive relation between riverbank erosion and migration. However, it can be interpret that farmers mostly affected due to riverbank erosion and migrate more, because farmers mostly inhabited in floodplain areas.
Does persistent meditation and physical activity mediate the relationship between perceptions of COVID-19 and mental health? A cross sectional survey of mobile app users in the United States.
Authors: Green Jeni , Huberty Jennifer , Puzia Megan , Stecher Chad 
Published: August 2020

Abstract
Background: The Coronavirus Disease 2019 (COVID-19) pandemic has been declared an international public health emergency with potential long-lasting effects on mental health. The purpose of this survey was to examine the regional differences in mental health and perceptions of COVID-19 in light of the state-level prevalence of COVID-19 cases, estimate the associations between perceptions of COVID-19 and health behavior engagement (i.e., physical activity, mindfulness meditation) and mental health, and explore the mediating effect of health behavior engagement on the relationship between perceptions of COVID-19 and mental health.Methods: A cross-sectional survey was distributed to a sample of US adult paying subscribers to the Calm app (data collection April 22 to June 3, 2020). The survey assessed perceptions of COVID-19, health behavior engagement, and mental health (i.e., perceived stress, post-traumatic stress disorder, and anxiety and depression). All statistical analyses were performed using R software. Differences in perceptions of COVID-19 and mental health by location were assessed using t-tests and chi-square tests. Logistic and OLS models regressed mental health and health behavior on COVID-19 perceptions, and Causal Mediation Analysis was used to estimate the significance of mediation effects. Results: Median age (N=8392) was 47 (SD=13.8) years. Mid-Atlantic region participants reported higher levels of stress, more severe depression symptoms, were more worried about COVID-19, paid more attention to COVID-19 news, and had more stress related to social distancing recommendations than participants living in other regions. The relationship between worry about COVID-19 and perceived stress was significantly mediated by changes in physical activity (p<.001) and strength of meditation habit (p<.001). The relationship between worry about COVID-19 and PTSD symptoms was significantly mediated by changes in physical activity (p<.001) and strength of meditation habit (p<.001). Conclusions: Our findings describe the mental health impact of COVID-19 and outline how continued participation in health behaviors such as physical activity and mindfulness meditation may buffer against worsening mental health due to the COVID-19 pandemic. These data have important implications for public health agencies and health organizations to promote the maintenance of health habits to reduce the residual mental health burden of the COVID-19 pandemic.
Accuracy of remote diagnoses using intraoral scans captured in approximate true color: a pilot and validation study in teledentistry
Authors: Steinmeier Sabrina Jenny , Wiedemeier Daniel , Hämmerle Christoph HF , Mühlemann Sven 
Published: August 2020

Abstract
Background: Intraoral scans (IOS) provide three-dimensional images with approximate true colors representing a possible tool in teledentistry for remote examination. The aim of the present cross-sectional validation study was, therefore, to evaluate the levels of agreement between remote diagnoses derived from IOS and diagnoses based on clinical examinations for assessing dental and periodontal conditions.Methods: The test sample comprised 10 patients representing different clinical conditions. Following the acquisition of IOS (Trios, 3Shape), a full-mouth dental and periodontal examination was done and periapical radiographs were taken. Ten dentists were asked to perform dental and periodontal scorings for each of the ten patients on a tablet computer presenting the IOS. Scores included diagnosis of gingivitis/periodontitis, and evaluated presence as well as amount of plaque and calculus, and presence of teeth exhibiting gingival recession, furcation involvement, erosion, tooth wear, stain, and non-carious cervical lesion, as well as presence of decayed, filled, and crowned teeth and implants. In a second round of assessments, the periapical radiographs were provided and the dentists were able to change the scores. The time for the remote assessment was recorded. The agreement between remote and clinical scorings (reference) was then analyzed descriptively.Results: The mean time for the tele assessment was 3.17 minutes and the additional consultation of the radiographs accounted for another 1.48 minutes. The sensitivity and specificity values were 0.61 and 0.39 for gingivitis and 0.67 and 0.33 for periodontitis, with no relevant changes when radiographs were provided for the diagnosis of periodontitis (0.72 and 0.28). The agreement for dichotomized dental and periodontal indices ranged between 78 and 95%. With the provision of radiographs, the remote examiners were able to detect existing filled teeth, crowned teeth, and implants, whereas the detection of decayed teeth (70%) was not improved.Conclusions: The remote examination using IOS was effective in detecting dental findings, whereas periodontal conditions could not be assessed with the same accuracy. Still, remote assessment of IOS would allow a time-efficient screening and triage of patients. Improvement of the image quality of IOS may further allow to increase the accuracy of remote assessments in dentistry.Trial registration: BASEC-Nr. Req-2019-01277
Mediating role of prenatal depression in attachment and maternal-fetal attachment in women who receive prenatal education
Authors: Zhang Ling , Wang Lei , Yuan Qiuyu , Cui Shu , Huang Cui , Zhang Kai , Zhou Xiaoqin 
Published: August 2020

Abstract
Background

Prenatal depression and attachment are factors that affect the establishment of an intimate relationship between a mother and fetus, and prenatal education plays an important role in helping pregnant women adapt to a maternal role and changes during pregnancy. The study explored differences in prenatal depression and maternal-fetal attachment (MFA) scores between a prenatal education group and no prenatal education group, as well as the effects of maternal depression scores and attachment dimensions on maternal intimacy with the fetus.
Methods

The Edinburgh Postnatal Depression Scale (EPDS), Experience of Close Relationship (ECR) scale, Maternal Antenatal Attachment Scale (MAAS) and a general data scale were used to investigate 222 pregnant women who received prenatal education and 118 pregnant women who did not receive prenatal education in the third trimester of pregnancy. An exploratory analysis was performed to analyze the effects of depression score and attachment on MFA in pregnant women who received prenatal education.
Results

The results showed that pregnant women who received prenatal education had higher MFA and lower depression scores than those who did not receive prenatal education, and prenatal depression partially mediated the relationship between attachment avoidance and MFA quality.
Conclusions

Women who received prenatal education had low prenatal depression scores and high MFA. Maternal depression and attachment avoidance may affect the emotional bond between mother and fetus and should be taken seriously; they can be prevented by prenatal education and early intervention.