bluuebunny/crossref_metadata_2025_split huggingface dataset:
Me-LLaMA: Medical Foundation Large Language Models for Comprehensive Text Analysis and Beyond
Authors: Xie Qianqian , Chen Qingyu , Chen Aokun , Peng Cheng , Hu Yan , Lin Fongci , Peng Xueqing , Huang Jimin , Zhang Jeffrey , Keloth Vipina , Zhou Xinyu , Qian Lingfei , He Huan , Shung Dennis , Ohno-Machado Lucila , Wu Yonghui , Xu Hua , Bian Jiang 
Published: December 2024

Abstract

Recent advancements in large language models (LLMs) like ChatGPT and LLaMA have shown significant potential in medical applications, but their effectiveness is limited by a lack of specialized medical knowledge due to general-domain training. In this study, we developed Me-LLaMA, a new family of open-source medical LLMs that uniquely integrate extensive domain-specific knowledge with robust instruction-following capabilities. Me-LLaMA comprises foundation models (Me-LLaMA 13B and 70B) and their chat-enhanced versions, developed through comprehensive continual pretraining and instruction tuning of LLaMA2 models using both biomedical literature and clinical notes. Me-LLaMA utilized the largest and most comprehensive medical data, including 129B pre-training tokens and 214K instruction tuning samples from diverse biomedical and clinical data sources. Training the 70B models required substantial computational resources, exceeding 100,000 A100 GPU hours. We applied Me-LLaMA to six medical text analysis tasks and evaluated its performance on 12 benchmark datasets. To further assess Me-LLaMA’s potential clinical utility, we evaluated its performance on complex clinical case diagnosis compared with other commercial LLMs, using both automatic and human evaluations. Me-LLaMA models outperform LLaMA, and other existing open-source medical LLMs in both zero-shot and supervised learning settings for most text analysis tasks. With task-specific instruction tuning, Me-LLaMA models also surpass leading commercial LLMs, outperforming ChatGPT on 7 out of 8 datasets and GPT-4 on 5 out of 8 datasets. Moreover, Me-LLaMA’s performance is comparable to ChatGPT and GPT-4 for diagnosing complex clinical cases. Our findings underscore combining domain-specific continual pretraining with instruction tuning is essential for developing effective domain-specific large language models in healthcare, significantly enhancing performance across diverse medical text analysis tasks and applications. By publicly releasing our models and resources under appropriate user agreements, we aim to foster innovation and facilitate advancements in medical AI, benefiting researchers and practitioners within the community.
Analysis of risk factors for gastrointestinal bleeding in percutaneous coronary intervention patients treated with dual antiplatelet therapy after surgery
Authors: Chen Caiping , Fan Huimin , Shuai Renya 
Published: December 2024

Abstract

Background: Following percutaneous coronary intervention (PCI), dual antiplatelet therapy (DAPT) is key to preventing thrombosis. However, DAPT use is strongly associated with an increased risk of Gastrointestinal bleeding. This complication not only affects the patient's recovery but also increases the healthcare burden. Therefore, identifying risk factors for Gastrointestinal bleeding in postoperative PCI patients is essential to optimise DAPT management and improve patient safety.
Objective:The aim of this study was to collect and analyse data from patients who underwent PCI at our hospital and were regularly treated with DAPT after the procedure. To determine the influence of previous medical history and factors during hospitalisation on the development of postoperative Gastrointestinal bleeding in patients undergoing PCI with DAPT, and to develop a risk prediction model accordingly. Provide cardiovascular physicians with a more accurate basis for identifying high-risk patients and guiding treatment.
Methods: A total of 380 patients were included in this study, out of which 42 patients presented with Gastrointestinal bleeding. Pre- and postoperative data and past medical history of patients were collected to study the correlation factors affecting the emergence of Gastrointestinal bleeding postoperatively in patients undergoing PCI with DAPT, and to establish a prediction model.
Results:Potentially relevant factors were included in the Univariate Logistic Regression Analysis. After analysis of the results: gender, age, History of Bleeding history, History of cancer, Smoking history, History of Heart failure, History of ProtonPump Inhibitors, Renal insufficiency, and Hypoproteinemia are the nine P < 0.2, which are potential risk factors that may affect the occurrence of Gastrointestinal bleeding after surgery in patients who underwent PCI with DAPT treatment. The data obtained were further included in a multifactorial logistic regression analysis: six factors such as gender (female), History of cancer, History of bleeding, History of cancer, History of smoking, Heart failure, and Renal insufficiency were found to be independent risk factors influencing the development of Gastrointestinal bleeding postoperatively in patients undergoing PCI with DAPT (P < 0.05).
Conclusion: The findings of this study confirmed six factors such as gender (female), History of Bleeding history, History of cancer, Smoking history, Heart failure and Renal insufficiency as independent risk factors for postoperative development of Gastrointestinal bleeding in PCI patients undergoing DAPT. The predictive model developed in this study can help cardiovascular physicians to some extent to accurately identify patients at high risk of bleeding and to be more cautious in the treatment as well as in the administration of medication.
The Influence of Thermal Tempering on the Fracture Resistance of Four Heat Pressed Lithia-based glass ceramic Crowns
Authors: Nasser Khaled , EL-Etreby Amr S. , Nabih Soha Osama 
Published: December 2024

Abstract

Background This in-vitro study aimed to evaluate the impact of thermal tempering and ceramic type on the fracture resistance of four heat-pressed glass ceramics.
Methods A total of 84 glass-ceramic crowns were pressed and randomly allocated into four equal groups (n=21) according to the ceramic type: Group (E): IPS e.max Press, Group (L): GC initial LiSi Press, Group (C): Celtra Press and Group (A): VITA Ambria. The crowns of each group were equally allocated into three subgroups (n = 7) regarding the subsequent thermal tempering temperature. Subgroup (T0): No tempering. Subgroup (T1): Tempering at 9% below pressing temperature. Subgroup (T2): Tempering at 5% below pressing temperature. Samples were tested for fracture resistance using a universal testing machine. A scanning electron microscope, X-ray diffraction and Energy Dispersive x-ray analysis were utilized to disclose the microstructural features.
Results When there is no tempering, IPS e.max press showed a significant elevated fracture resistance. There was an insignificant difference between other ceramics. While with tempering (T2) as well as (T1), Lisi press (L) showed a significant elevated fracture resistance. There was an insignificant difference between other ceramics.
Conclusions Incorporation of zirconia oxide into the lithium disilicate glass matrix did not show improvement in the fracture resistance. Thermal tempering procedure had significant effect on fracture resistance.
ARMH4 accelerates aging by maintaining a positive-feedback growth signaling circuit
Authors: Wang Zhihua , Fang Yu , wang baosen , Guo Qiuxiao , Lv Jian , Li Jiajie , Wang Junmei , Guo Ningning , Bai Yu , Xu Anqi , Ma Xiao , Tan Hui , Li Hong 
Published: December 2024

Abstract

Aging is an inevitable process integrating chronological alterations of multiple organs. A growing aging population necessitates feasible anti-aging strategies to deal with age-associated health problems. We previously performed a proteomics analysis in a healthy-aging cohort, and revealed an age-related downregulation of ARMH4. Here we generated a whole-body Armh4-knockout mouse line, and investigated its impact on systemic aging. Under normal feeding conditions, Armh4 deficiency significantly lowered spontaneous mortality and extended maximum lifespan. In the female mice, Armh4 deficiency postponed sexual maturity for one week. At the organ level, the age-related pathologies of the heart, liver, kidney, and spleen were substantially alleviated by Armh4 deletion. Mechanistically, ARMH4 interacted with IGF1R/FGFR1 to sensitize the activation of PI3K-AKT-mTORC1 and Ras-MEK-ERK pathways, consequently promoting protein synthesis and inhibiting autophagy. Moreover, ARMH4 was required for the maintenance of IGF1R/FGFR1 expressions through regulating transcription factor c-Myc. Therefore, ARMH4 maintains a positive-feedback growth signaling to promote aging.
Assessing the variability effect of land use patterns, rainfall and temperature trends on land degradation across Ibadan, Nigeria
Authors: Areola Abiodun Ayooluwa 
Published: December 2024

Abstract

Land degradation remains a significant global challenge, affecting ecosystems, agriculture, and human livelihoods. This study employed remote sensing technology to investigate vegetation patterns and trends in rainfall and temperature in Ibadan, Nigeria, from 2013 to 2023, with a focus on their impact on land degradation. The objectives were to assess spatiotemporal changes in vegetation cover, temperature, and rainfall distribution; identify areas affected by land degradation; and evaluate the influence of weather variables on vegetation dynamics. Data from NASA Power and the Nigeria Meteorological Agency (NIMET) were combined with Landsat 8 imagery processed using Google Earth Engine (GEE). The analysis revealed substantial rainfall variability across Ibadan, with higher levels in the northern and central regions affecting vegetation health, as reflected by the Normalized Difference Vegetation Index (NDVI). Land surface temperature (LST) analysis indicated a moderate negative correlation with NDVI (r = -0.39), demonstrating that rising temperatures adversely impact vegetation health. Additionally, a weak positive correlation (r = 0.11) between land use and land cover (LULC) and rainfall highlighted the role of urbanisation in shaping temperature and precipitation patterns. The findings underscore the importance of sustainable urban planning and land management strategies, including green infrastructure and eco-friendly building practices, to mitigate the effects of weather variability on vegetation and land degradation.
The miR-3156-3p/K+ channel axis regulates replication and release of non-enveloped RNA viruses
Authors: Shih Shin-Ru , Lin Yueh-Te , Kung Yu-An , Gong Yu-Nong , Tang Wen-Fang , Horng Jim-Tong 
Published: December 2024

Abstract

Viruses rely on egress machinery for cell exit, which is crucial for their transmission. While non-enveloped RNA viruses, such as picornaviruses, are typically associated with the lytic release pathway, emerging evidence suggests they can also be packaged within vesicles for non-lytic release. However, the molecular mechanisms regulating this remain unclear. Here, we performed a genome-wide CRISPR-Cas9 screen and identified the microRNA hsa-miR-3156-1 as a critical host factor for picornavirus replication. Both the primary and mature forms of miR-3156-1 were upregulated following infection, with miR-3156-3p serving as a positive regulator of viral replication by directly targeting the 3′-untranslated regions of K+ channel-associated genes, KCNV1, KCNJ2, KCNK3, and KCNK5, causing plasma membrane depolarization and Ca2+ influx, which facilitate non-lytic release. Administration of a miR-3156-3p antagomir alleviated virus-associated acute flaccid paralysis in a mouse model. Our findings reveal how the miR-3156-3p/K+ channel regulatory axis regulates picornavirus release, highlighting potential therapeutic targets.
Face parsing and blending-based analysis model for makeup style transfer
Authors: Wang Xiaofeng , Zhang YiChen , Han Tianbo , Liu Wei , Liu Songling , Zhang Yongqin , Liu Yonghuai 
Published: December 2024

Abstract

Makeup is a fundamental element of traditional opera culture, embodying profound artistic connotations and aesthetic values. Incorporating patterns and decorative elements into makeup has also become an essential aspect of contemporary society. Makeup transfer technology enables the reproduction of existing makeup designs onto specified facial images. However, current methodologies face several challenges, including incomplete pattern replication and suboptimal results, particularly in transferring patterned makeup. Consequently, this paper proposes a novel makeup transfer model, termed Opera-MT, which integrates facial parsing with image-fusion techniques. The Opera-MT model first maps facial images onto UV space to establish a pixel-accurate correspondence between the reference face and the source face, effectively addressing issues related to pattern distortion. Subsequently, it integrates facial parsing techniques with Poisson image fusion methods to preserve the texture details of the pattern, thereby overcoming the challenge of incomplete pattern migration in the reference makeup. Finally, the model utilizes a color transfer network to apply the color style of the reference makeup to the source face, resulting in the final output. The effectiveness of Opera-MT has been validated on three datasets, including our collected PM dataset and two publicly available datasets, namely the MT and makeup datasets. The results demonstrate that Opera-MT successfully captures makeup color and pattern texture, producing high-quality, realistic, and natural results. Our code will be available at https://github.com/xiaozhang0927/Opera-MT.
Melittin induces apoptosis, Mitochondrial membrane potential, Reactive Oxygen species, and cell cycle arrest against T-cell acute lymphoblastic leukemia (T-ALL) in vitro.
Authors: Kumar Ajeet , Tilak Vijai , Raju S.V.S , Ali Akhtar 
Published: December 2024

Abstract

Acute lymphoblastic leukemia originating from early T-cell progenitors is called T-cell acute lymphoblastic leukemia (T-ALL). Chemoresistant T-ALL cancer is gaining interest in less hazardous drugs and therapy. These days, pyrimidine and phytochemical analogs have unique qualities that can be used to develop novel medications. Research has shown that melittin has cytotoxic effects on cancer, however, these effects are yet unclear. Our present study aims to evaluate melittin’s ability to induce apoptosis in Acute lymphoblastic leukemia cells. Our goal was to find out how Melittin affected the DNA fragmentation test, hemolytic assay, reactive oxygen species, cell proliferation, and apoptosis in Jurkat cells. The effects of Melittin on apoptosis were examined using propidium iodide and acridine orange. The effects of Melittin on cell viability were determined by (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) MTT assay for 24–48 h. Using MTT, we assessed Melittin's cytotoxic activity on Jurkat cells and established the drug's IC50 value (1µg/mL). Melittin suppressed the growth of Jurkat cells in a dose-dependent manner, as the results demonstrate. Melittin promoted pro-apoptotic genes and downregulated anti-apoptotic ones to cause apoptosis. Our findings demonstrated that different doses of Melittin decreased cell viability in Jurkat cells, cell cycle arrest in G2-M Phase, Annexin-V PI apoptosis in late apoptotic and Mitochondrial membrane potential decrease in Rh-123 fluorescence intensity indicates disrupted membrane potential compared to the control. According to our research, melittin may be an effective anti-tumor agent in ALL by inhibiting the induction of apoptosis and reducing antioxidant levels in Jurkat cells.
Application of fertigation using biotreated agro-industrial wastewater (with Bacillus paramycoides MT477810) on the growth of two vegetables Capsicum annuum (var. Astra) and Lycopersicon esculentum (var. Kristina)
Authors: Afzal Aqsa , Mirza Safdar Ali , Rashid Aneeba , Aslam Tahira , Campos Luiza C. 
Published: December 2024

ABSTRACT

This research explored the use of the fertigation technique with treated agro-industrial wastewater to enhance the growth of Capsicum annuum (var. Astra) and Lycopersicon esculentum (var. Kristina). The wastewater, collected from Bajwa Agro Industries in Lahore, was biotreated using the bacterial strain Bacillus paramycoides MT477810, with a 10% inoculum incubated at 37 °C for 48 h. The bacterial strain achieved an 87% decolorization of the wastewater. Physicochemical analysis before and after biotreatment showed reductions in pH (60%), EC (22%), salinity (16%), turbidity (48%), COD (40%), BOD (73%), TDS (62%), and TSS (25%). The two vegetable varieties were fertigated with both biotreated and untreated wastewater to assess their effects on seed germination and plant growth. Results indicated a significant reduction in phytotoxicity (over 50%) in plants grown with biotreated wastewater. Additionally, plants irrigated with biotreated wastewater showed a 9–30% increase in fresh and dry weights compared to those irrigated with untreated wastewater. Nutritional analysis revealed that crops irrigated with treated wastewater had 12–48% higher nutrient content. These findings emphasize the effectiveness of biological wastewater treatment and fertigation in reducing pollutants and enhancing nutrient availability for vegetable crops.
Water balance and hydropluviometric variability of dams in Eastern Algeria
Authors: Boutouatou Farah , Mebarki Azeddine , Laignel Benoit , Nouaceur Zeineddinne 
Published: December 2024

ABSTRACT

Eastern Algeria, the wettest region of the country, has experienced a vast program of dam construction, distributed across basins marked by strong physio-climatic disparities. The hydrological functioning studied through the use of data from the regulation balance sheet for the period from September 1990 to August 2013, concerned a sample of 10 dams (capacity varying from 3 to 200 hm³) whose year of impoundment varies from 1939 to 1987. The results of the statistical and spectral analysis applied to two main terms of the water balance (inputs and rainfalls) indicate a strong relationship between the studied parameters, reflecting a net increase in dry years from September 1990 to August 2002, with a return of wet years from the year 2003, however, interrupted by other very dry years like that of 2007/2008. The outflows (outputs) of the dams reflect considerable water losses in the form of evaporation and also in the form of leaks on karstic sites. However, it is the flood phenomena that are at the origin of remarkable overflows and bottom outlets. In addition to these losses, there are significant variations in the regulated volume of dams, particularly in semi-arid zones and during periods of drought, particularly.
Microbiological Assessment and Evaluation of Virus Yield in Germ-free Chicken Eggs
Authors: Adeyemi Oluwapelumi , dos Santos Ariane G B , Sadeyen Jean-Rémy , Carter Toby , Murphy Martin , Caulfield Catherine , Moran Leonard , Adeyemi Modupeore , Iqbal Munir 
Published: December 2024

Background
Increasing yield will have major implications for vaccine manufacturers. Internal bacterial contamination of specific pathogen-free (SPF) eggs results in batches of vaccines being rejected and may negatively impact vaccine yield.

Objective
The objectives of this study were to analyze Germ-Free (GF) eggs microbiologically and evaluate the yield of H1N1 when compared with SPF eggs from 2 different suppliers.

Methods
The contents of GF eggs were analyzed using three different methods (sterility test, total viable count, and bioluminescence). Eggs from each source were inoculated with H1N1 virus. Chick Embryo Fibroblast (CEF) cell cultures were generated from GF and SPF eggs from both suppliers and infected with H1N1. The supernatant was titrated for viral Haemagglutinin activity and virus infectivity while cellular RNA extracts were assayed for Interferon (α and β) gene expression by RT-qPCR using gene-specific primers.

Results
The analysis of GF eggs confirmed that the contents were sterile. The batch yield of GF eggs was ~1.2-fold and ~1.8-fold higher than SPF supplier 1 and SPF supplier 2 virus yields, respectively. Replication of H1N1 was 2-fold higher in CEF derived from GF eggs than CEF derived from SPF supplier 1 or SPF supplier 2. IFN-β antiviral response of GF-derived CEF cells was 2-fold lower than SPF supplier 1-derived and ~3-fold lower than SPF supplier 2-derived CEF cells.

Conclusion
The content of GF eggs was sterile. The yield of H1N1 was higher in GF eggs than in SPF eggs, and the higher yield was associated with a significant reduction in interferon Beta levels.
TRABALHO E EDUCAÇÃO: INTERFACES ENTRE PROCESSOS EDUCATIVOS, SABERES SOCIOAMBIENTAIS E EXPERIÊNCIAS DE TRABALHO DA COLETA DO AÇAÍ NO MUNICÍPIO DE IGARAPÉ-MIRI/PA
Authors: Cardoso da Silva Rodrigo 
Published: December 2024

Trata-se de uma pesquisa que tem como fenômeno social de estudo, processos educativos e saberes socioambientais que se configuram, informam e/ou orientam experiências de trabalho da coleta do açaí, no nordeste da Amazônia paraense. A referida pesquisa integra a linha de pesquisa Saberes Culturais e Educação na Amazônia, do Programa de Pós-Graduação em Educação da Universidade do Estado do Pará (PPGED-UEPA).
Trata-se de saberes socioambientais e processos educativos que sustentam relações não predatórias com a natureza e conformam modos de vida demarcados pela dualidade estrutural no processo de produção e reprodução à existência das lideranças na comunidade, que com o passar do tempo, essas práticas de trabalho realizadas pelos moradores passaram a incorporar novos processos educativos e saberes socioambientais, em decorrência as mudanças que foram ocorrendo na dinamização da produção capitalista e nas suas inovações tecnológicas. E para não concluir, os sujeitos históricos da comunidade Salento lutam e (re) existem com as vozes contra a estrutura opressora, eurocêntrica, negacionistas e colonialista, imposta pelo capital. Portanto, trata-se de um modo de vida que desafia a ciência e a educação.
TRABALHO-EDUCAÇÃO, EXPERIÊNCIA DE CLASSE E RELAÇÕES PEDAGÓGICAS TECIDAS NOS ESPAÇOS DE OCUPAÇÃO DO MOVIMENTO DOS TRABALHADORES SEM-TETO (MTST)
Authors: Gomes Luiz Augusto de Oliveira 
Published: December 2024

O esforço de síntese aqui apresentado é fruto da trajetória de pesquisa realizada no Doutorado em Educação, da Faculdade de Educação, na Universidade Federal Fluminense, sob orientação de Lia Tiriba. Nosso trabalho de investigação junto ao Movimento dos Trabalhadores Sem-Teto surge a partir da minha inserção como coordenador estadual do movimento, acompanhando os espaços de ocupação, tais como assembleias, Cozinhas Solidárias, atos públicos e ocupações de moradia. Partindo da premissa de que a questão da habitação é um elemento fundamental para a garantir existência humana, o objetivo da pesquisa foi o de analisar processos de ‘ocupação’ do Movimento dos Trabalhadores Sem Teto (MTST), entendidos como experiência de classe e como mediação na formação política de homens e mulheres que integram a parcela mais precarizada da classe trabalhadora.
Palavras-Chave: Trabalho-educação. Movimentos sociais. Ocupação. Experiência de classe. Relações pedagógicas.