bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long interspersed nuclear element 1 hypomethylation has novel prognostic value and potential utility in liquid biopsy for oral cavity cancer
Authors: Misawa Kiyoshi , Yamada Satoshi , Mima Masato , Nakagawa Takuya , Kurokawa Tomoya , Imai Atsushi , Mochizuki Daiki , Shinmura Daichi , Yamada Taiki , Kita Junya , Ishikawa Ryuji , Yamaguchi Yuki , Misawa Yuki , Kanazawa Takeharu , Kawasaki Hideya , Mineta Hiroyuki 
Published: August 2020

Abstract
Background New biomarkers are urgently needed to improve personalized treatment approaches for head and neck squamous cell carcinoma (HNSCC). Global DNA hypomethylation has wide-ranging functions in multistep carcinogenesis, and the hypomethylation of long interspersed nucleotide element-1 (LINE-1) has been generally considered to be related with increased retrotransposon activity and induced genome instability. However, little information is available regarding LINE-1 hypomethylation and its prognostic implications in HNSCC. Methods In this study, we analyzed LINE-1 hypomethylation levels in a well-characterized dataset of 317 matched pairs of HNSCC tissues and oral cavity cancer (OCC) circulating tumor DNA (ctDNA) using quantitative real-time methylation and unmethylation PCR. This analysis was performed according to various clinical characteristics and prognostic implications. Results Our results demonstrated that LINE-1 hypomethylation levels were significantly higher in HNSCC tissues than in corresponding normal tissues from the same individuals (P < 0.001). Univariate analysis revealed that high levels of LINE-1 hypomethylation were correlated with poor disease-free survival (DFS; log-rank test, P = 0.038), whereas multivariate analysis demonstrated that it is a significant independent prognostic factor for DFS (hazard ratio: 2.10, 95% confidence interval: 1.02–4.36; P = 0.045). Moreover, patients with high LINE-1 hypomethylation levels exhibited the greatest decrease in 5-hydroxymethylcytosine (5-hmC) levels and increase in tumor-suppressor gene methylation index (P = 0.006 and P < 0.001, respectively). Further ctDNA studies also showed that LINE-1 hypomethylation had high predictive ability for OCC. Conclusions LINE-1 hypomethylation is associated with a higher risk of early OCC relapse, and is hence, a potential predictive biomarker for OCC. Furthermore, 5-hmC levels also exhibited predictive potential in OCC, based on its inverse correlation with LINE-1 hypomethylation levels. LINE-1 hypomethylation analysis, therefore, has applications in determining patient prognosis and real-time surveillance for disease recurrence, and could serve as an alternative method for OCC screening.
Oral Health Matters- Tooth Problem and its Treatment-seeking Behaviour Among Older Adults in India
Authors: Srivast Shobhit , Kumar Pradeep , Sinha Debashree , Mishra Prem Shankar 
Published: August 2020

Abstract
Background: Growing untreated morbidities among the older adults on geriatric issues especially an optimal dental treatment has become an unfinished agenda and a long-neglected area for care and management in many low and middle-income countries. There is a continuous rise in the older population in India and at the same time, the rising of co-morbidities or multi-morbidities in the aging population creates catastrophic challenges at the individuals, household, community, and country levels, and therefore, it is important to address dental health problems and related issues in the aged population in India. The study aims to understand the prevalence of dental health problems and their health-seeking behaviour among older adults in India. Further, the study determines the factors associated with low access and under-coverage of dental health care services among the elderly populations in IndiaMethods: The present research used data from Building a Knowledge Base on Population Aging in India (BKPAI) which was a national level survey and was conducted in 2011, across seven states of India. The effective sample size of this study was 9541 older adults aged 60+ years. Descriptive statistics and bivariate analysis were used to fulfill the study objectives. Additionally, the study employed the Heckprobit selection model which is a two-equation model to understand the determinants of dental health problems.Results: Older adults in the age group of 70-79 years (36.9%) and 80+ years (51.9%) reported higher dental problems compared to 60-69 years (19.9%), older women reported more dental problems (29.2%) and also used more dental aids (12.6%) compared to older men. Older adults in the age group 70-79 and 80+ years were 0.15 times and 0.40 times less likely to use dental aids respectively, as compared to 60-69 years older adults. Moreover, older women were 0.28 times more likely to use dental aids than older men. Education and wealth of older adults have a negative relationship with dental problems.Conclusion: This study clearly emphasized that awareness of smoking-related health problems should be promoted vigorously. Further, a holistic approach is needed to prevent dental diseases that occur because of other co-morbidities. For that, integration of the oral health program with programs dealing with morbidities can act as a solution to the existing problem.
Fabrication and Characterization of Copper Nanoparticles by Green Synthesis Approach Using Plectranthus Amboinicus Leaves Extract
Authors: Parthasarathy Srividya , Jayacumar Sanjana , Chakraborty Sudestna , Soundararajan Prathyusha , Joshi Darshani , Gangwar Kaumudi , Bhattacharjee Astha , Venkatesh M.Pandima Devi 
Published: August 2020

Abstract
The field of nanotechnology is gaining interest among the researchers towards the eco-friendly way of synthesis of nanoparticles. In this project, green synthesis technique was employed to induce the synthesis of copper nanoparticles using Plectranthus amboinicus, i.e. Mexican mint, identified as Coleus amboinicus leaf extract. We report an eco-friendly synthesis of copper nanoparticle using Plectranthus amboinicus leaf extract, which is a simple and an ostentatiously rapid method which produces stable nanoparticles. The copper sulphate solution was naturally employed as a precursor for synthesizing the copper nanoparticles. The extract of the plant Plectranthus amboinicus was found to showcase excellent reducing and stabilizing properties. By using Ultraviolet-Visible spectroscopy, Zeta Potential, and X-Ray Diffraction (XRD) studies, it was confirmed that copper nanoparticles have been synthesized. The UV-Spectrometer analysis shows the characteristic peak indicating the synthesis of copper nanoparticles. The pattern of XRD analysis showed particle size of 16 - 25 nm and it reveals high crystallinity of the copper nanoparticles. Zeta potential was done to find the charge of the nanoparticles and size distribution which showed to have significant stability. This method proves to be cost-effective, can be performed at ease, and it’s also free of pollutants.
High Serum Asprosin Levels Are Associated with Presence of Metabolic Syndrome
Authors: Hong Tao , Li Jiao-Yang , Wang Ya-Di , Qi Xiao-Yan , Liao Zhe-Zhen , Bhadel Poonam , Ran Li , Yang Jing , Yan Bin , Liu Jiang-Hua , Xiao Xin-Hua 
Published: August 2020

Abstract
Objective: Asprosin, a new adipocytokine, has been reported to be related with glucose release, dyslipidemia and insulin resistance (IR). However, the relationship of asprosin with metabolic syndrome (MetS) remains unknown. This study aims to investigate serum asprosin levels in MetS as well as its association with various metabolic parameters in humans.Methods: The consecutive 131 patients with MetS and age-matched 162 healthy subjects were recruited for this study. Serum asprosin concentrations were determined by ELISA. Lipid profile, glucose, insulin, and inflammatory markers were also measured.Results: Serum asprosin levels were higher in subjects with MetS 23.52 (16.70, 32.05) ng/ml compared to 16.70 (12.87, 22.38) ng/ml in the controls (P < 0.01), and showed an increasing trend with the increased numbers of metabolic components (P for trend < 0.01). In all studied subjects, serum asprosin levels were positive correlated with body mass index, waist circumference, percentage of body fat (%), triglyceride, fasting plasma glucose, 2h plasma glucose, fasting insulin, HOMA-IR, interleukin (IL) -6 and monocyte chemoattractant protein (MCP)-1, and negative correlated with HDL cholesterol (P < 0.05). In a multiple linear regression, asprosin was independently and positively correlated with triglyceride and HOMA-IR (P < 0.05). Binary logistic regression revealed that asprosin was independently and positively correlated with the occurrence of MetS and IR even after controlling for anthropometric variables, lipid profiles and inflammatory markers. Conclusion: Asprosin may be a metabolic - related adipokine and related to insulin resistance and MetS. Trial Registration: ChiCTR, ChiCTR1800018347. Registered 12 September 2018, http://www.chictr.org.cn/showproj.aspx?proj=31050.
Age Distribution and Associated Factors of Cornea Biochemical Parameter Stress-Strain Index in Chinese Healthy Population
Authors: Liu Guihua , Rong Hua , Pei Ruxia , Du Bei , Jin Nan , Wang Di , Jin Chengcheng , Wei Ruihua 
Published: August 2020

Abstract
Background: To investigate the new cornea biochemical parameter stress-strain index (SSI) in Chinese healthy people and the factors associated with SSIMethods: A total of 175 eyes from 175 participants were recruited in this study. Axial length was measured with the Lenstar LS-900. Pentacam was used to measure curvature of the cornea and ACV and cornea biomechanical properties were measured by corneal visualization Scheimpflug technology (Corvis ST). Student’s t-test, one-way ANOVA, univariate and multivariate linear regression were used in the statistical analysis.Results: The mean (±SD) SSI was 1.14 ± 0.22 (range, 0.66–1.78) in all subjects and affected by age significantly after age of 35 (P < 0.05). In univariate regression models, SSI did not vary with biomechanical intraocular pressure (bIOP) (P=0.989), steepest radius of anterior corneal curvature (RsF) (P=0.984) or central corneal thickness (CCT) (P=0.651). In multivariate regression models, SSI was significantly associated with age (β=0.557, P<0.001), axial length (AL) (β=-0.550, P<0.001), intraocular pressure (IOP) (β=0.377, P<0.001) and flattest radius of anterior corneal curvature (RfF) (β=0.222, P<0.001) but not with anterior chamber volume (ACV).Conclusions: SSI was increased by age after the age of 35. In addition to age, SSI and RfF, IOP is positively correlated and negatively correlated with AL.
Group-level Regional Cerebral Uptake Quantification in Micro PET-CT
Authors: Leatherday Christopher , Lam Virginie , Boucek Jan , Celliers Liesl 
Published: August 2020

Abstract
The lack of soft tissue CT contrast is an impediment to accurate quantification of regional cerebral uptake on micro PET-CT. Co-registration to an atlas can aid with quantitative analysis, particularly when MRI is not available. Methods: A subject CT is cropped to the skull, the brain void is then filled to create a “skull cavity mask”. An MRI mouse brain volumetric template is then co-registered to the skull cavity mask via affine transformation. Each subsequent subject CT is then skull cropped and co-registered to the first, these transformations are then applied to each corresponding PET for group level co-registration. Results: The method was tested on eight mice with each possible registration permutation; resulting in small variations in regional cerebral PET counts. Conclusion: We present a semi-automated method that allows for quantification of regional cerebral PET uptake from micro PET-CT images without subject MRI data.
Increased Risk of Brain Metastases Among Patients with Melanoma and PROM2 Expression in Metastatic Lymph Nodes
Authors: Nguyen Thuy Thi , Gapihan Guillaume , Tetu Pauline , Pamoukdjian Frédéric , Bouchtaoui Morad El , Lebœuf Christophe , Feugeas Jean-Paul , Paris Justine , Baroudjian Barouyr , Delyon Julie , Mourah Samia , Lebbé Céleste , Janin Anne , Bousquet Guilhem , Battistella Maxime 
Published: August 2020

Abstract
Background: Melanoma brain metastases are the main cause of specific death among patients with metastatic melanoma. The biology of melanoma brain metastases remains largely to be deciphered, as there have been only a few genomic studies on brain metastatic samples. In this study, melanoma metastatic lymph nodes were used with the aim to identify biomarkers associated with the occurrence of brain metastases. Methods: Fifty-one patients with melanoma lymph node metastasis and a median follow-up of 48 months were included in the development cohort. Transcriptomic data were obtained from these metastatic lymph nodes and patients who developed brain metastases and those who did not were compared. Recommendations for tumour marker prognostic studies (REMARK recommendations) were followed.Results: From transcriptomic data, we identified PROM2 which was significantly overexpressed in metastatic lymph nodes of patients who developed brain metastases compared to those who did not. Using immunohistochemistry with two different anti-PROM2 antibodies, a PROM2 score was developed for metastatic lymph nodes. Using a cut-off of 5, a PROM2 mean score ≥5 was significantly associated with an increased risk of brain metastases and an increased hazard risk of death by 4.These results were confirmed in an internal validation cohort of 50 additional patients with melanoma lymph node metastases.Conclusions: In this study, we identified PROM2 expression as a biomarker predictive of the occurrence of distant metastases, particularly brain metastases, among patients with stage III melanoma. Our findings open new perspectives to validate PROM2 as a useful biomarker for clinical trials in the adjuvant setting, and as a potential biotarget for the treatment of metastatic melanoma.
Understanding the implementation of evidence-informed policies and practices from a policy perspective: a critical interpretive synthesis
Authors: Bullock Heather L , Lavis John N , Wilson Michael G , Mulvale Gillian , Miatello Ashleigh 
Published: August 2020

Abstract
Background: The fields of implementation science and knowledge translation have evolved somewhat independently from the field of policy implementation research, despite calls for better integration. As a result, implementation theory and empirical work do not often reflect the implementation experience from a policy lens nor benefit from the scholarship in all three fields. This means policy-makers, researchers and practitioners may find it challenging to draw from theory that adequately reflects their implementation efforts.Methods: We developed an integrated theoretical framework of the implementation process from a policy perspective by combining findings from these fields using the critical interpretive synthesis method. We began with the compass question: how is policy currently described in implementation theory and processes and what aspects of policy are important for implementation success? We then searched 12 databases as well as grey literature and supplemented these documents with other sources to fill conceptual gaps. Using a grounded and interpretive approach to analysis, we built the framework constructs, drawing largely from the theoretical literature and then tested and refined the framework using empirical literature.Results: A total of 7850 documents were retrieved and assessed for eligibility and 34 additional documents were identified through other sources. Eighty-two unique documents were ultimately included in the analysis. Our findings indicate that policy is described as: 1) the context; 2) a focusing lens; 3) the innovation itself; 4) a lever of influence; 5) an enabler/facilitator or barrier; or 6) an outcome. Policy actors were also identified as important participants or leaders of implementation. Our analysis led to the development of a two-part conceptual framework, including process and determinant components. Conclusions: This framework begins to bridge the divide between disciplines and offers a new way of thinking about implementation processes at the systems level. It offers researchers, policy-makers and implementers with a new way of thinking about implementation and can be used for planning or evaluating implementation efforts.
Discoidin domain-containing receptor 2 is present in human atherosclerotic plaques and involved in the expression and activity of MMP-2
Authors: Qi Yu , Liu Ruihan , Chen Ying , Waqar Ahmed Bilal , Liu Fuqiang , Yang Juan , Lian Ting , Zhang Guangwei , Guan Hua , Cui Yuanyuan , Xu Cangbao 
Published: August 2020

Abstract
Background: DDR2 has been suggested to be involved in atherosclerotic progression, but its pathological role remains unknown. Methods: Using immunochemical staining, we located and compared the expression of DDR2 in the atherosclerotic plaques of humans and various animal models. Then, siRNA was applied to knockdown the expression of DDR2 in smooth muscle cells (VSMCs), and the migration, proliferation and collagen I-induced expression of matrix metalloproteinases (MMPs) were evaluated. Results: We found that an abundance of DDR2 was present in the atherosclerotic plaques of humans and various animal models and was distributed around fatty and necrotic cores. After incubation of ox-LDL, DDR2 was upregulated in VSMCs in response to such a pro-atherosclerotic condition. Next, we found that decreased DDR2 expression in VSMCs inhibited the migration, proliferation and collagen I-induced expression of matrix metalloproteinases (MMPs). Moreover, we found that DDR2 strongly associated with the protein expression and activity of MMP-2, suggesting that DDR2 might play a role in the etiology of unstable plaques. Conclusion: Considering that DDR2 is present in the atherosclerotic plaques of humans and is associated with collagen I-induced secretion of MMP-2, the clinical role of DDR2 in cardiovascular disease should be elucidated in further experiments.
A Nomogram to Predict Breast Tumor Regression Patterns after Neoadjuvant Chemotherapy Based on Baseline Characteristics
Authors: Zhuang Xiaosheng , Cheng Minyi , Zhang Liulu , Ji Fei , Zhu Teng , Yang Ciqiu , Yang Mei , Gao Hongfei , Li Jieqing , Wang Kun 
Published: August 2020

Abstract
Background Breast cancers show different regression patterns after neoadjuvant chemotherapy. Certain regression patterns are associated with more reliable margins in breast-conserving surgery. Our study aims to establish a nomogram based on clinicopathological factors and laboratory indicators to predict regression patterns in breast cancer patients.Methods We retrospectively reviewed 320 patients with stage II-III breast cancer who received neoadjuvant chemotherapy and had definitive surgery in our center from January 2016 to December 2019. Tumor regression patterns were categorized as type 1 (concentric regression + pCR) and type 2 (multifocal residues + SD + PD) based on mass pathological results. A multivariate logistic regression model was applied to distinguish predictive factors for tumor regression patterns. A nomogram was built based on five predictive factors from the multivariate logistic regression model.Results Molecular subtypes were not significantly associated with tumor regression patterns. Multivariate analysis identified five independent indicators: menopausal status, estrogen receptor status, T stage, N stage and lymphocyte to monocyte ratio. The nomogram yielded an area under the curve (AUC) of 0.70 (95% confidence interval 0.62-0.78) based on these factors. The calibration plot accompanied by the Hosmer–Lemeshow goodness-of-fit (GOF) test (p-value =0.9) showed good consistency between the estimated probabilities and the actual rate of type 1 cases.Conclusion HR+/HER2- breast cancers are more likely to have type 2 regression after neoadjuvant chemotherapy. Five baseline factors, including clinicopathological factors and laboratory indexes, were incorporated to establish a nomogram, which exhibited a satisfactory discriminatory ability for predicting different patterns of tumor regression.
Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy for Peritoneal Metastasis from Breast Cancer: A Preliminary Report on 4 Cases
Authors: Yu Jun-Hui , Feng Yu , Li Xin-Bao , Zhang Cheng-Yan , Shi Feng , An Song-Lin , Liu Gang , Zhang Yan-Bin , Zhang Kai , Ji Zhong-He , Li Bing , Yan Guo-Jun , Li Yan-Ping , Li Yan 
Published: August 2020

Abstract
Background: Breast cancer (BC) has the highest morbidity and the fifth mortality rate among women in China. Peritoneal metastases from BC is a rare disease and no guideline or international consensus for it. Objective: To summarise our experiences in cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) to treat breast cancer peritoneal carcinomatosis (BC PC).Methods: 4 BC PC patients underwent CRS+HIPEC were enrolled in this study. The clinic-pathologic characteristics and overall survival (OS) were collected and analysed.Result: The average age at CRS+HIPEC was 59.8 years. The average time of CRS+HIPEC was 8.8 h. The median number of resected organ areas was 7. OS from CRS+HIPEC were 31, 28, 16 and 52 months. There were no serious adverse events (SAEs) during the perioperative period.Conclusions: The 4 cases provided evidence that integrated therapy with CRS+HIPEC may be a promising strategy to improve the outcome for BC PC patients.
Upregulation of Bag3 Exacerbates Cervical Cancer Progression by Impairing Immune Response and Inhibiting Cell Ferroptosis
Authors: Zhang Yan , Zhang Jun , Jiang Yanping , Zhao Yuzi , Tan Aili , Wang Lu , Wang Shujun , Pi Jie , Mao Lin , Li Xing 
Published: August 2020

Abstract
Background: Cervical cancer remains a serious threat to women worldwide. Thus, effective strategies to treat cervical cancer are urgently needed. Bcl-2-associated athanogene 3 (Bag3) has been shown to be increased in several malignant neoplasms. However, little is known about the function of Bag3 in cervical cancer. We aimed to evaluate the function of Bag3 in cervical cancer progression.Method: qRT-PCR was carried out to test mRNA expression of Bag3, SLC7A11 and SLC3A2. Western blot analysis was conducted to detect protein expression of Bag3, SLC7A11 and SLC3A2. Cell proliferation was assessed using CCK-8, EdU and Colony formation assay. Flow cytometry assay was used to determine the frequency of IFN-γ- or TNF-α-producing CD8+ T cells. Transwell migration and invasion assay were carried out to detect cell migration and invasion capacity. Immunohistochemical staining was carried out to assess Bag3 and CD3 expressionResults: Bag3 was obviously elevated in cervical cancer tissues than in the adjacent normal tissues. Bag3 mRNA was upregulated in different cervical cancer cells (SiHa, C-33A, HT-3, and HeLa cells). SiHa cell proliferation, colony formation, and migration/invasion capacity were enhanced by Bag3 overexpression. Bag3 inhibited the immune response in cervical cancer. After C57BL6 mice were injected with Bag3-overexpressing SiHa cells, infiltrating CD3+ T cells around tumors were reduced. IFN-γ- and TNF-α-producing CD8+ T cells in tumor sections were remarkably inhibited by Bag3. Moreover, Ki-67+‑ and CD107a-producing CD8+ T cells were also suppressed. Bag3 repressed SiHa cell ferroptosis. Bag3 deletion inhibited cervical cancer development by improving the immune response and inducing ferroptosis. Conclusions: Taken together, these results indicated that Bag3 contributes to cervical cancer progression by impairing immune response and repressing cell ferroptosis.