bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Expression Levels of KIF18A is an Independent Predictor of Poor Survival in Glioma: A Study Using Multi Public Data
Authors: Chang Haigang , Wang Yaxiao , Li Yan , Yang Weilong , Wang Lei , Ma Pengju , Li Xinxiao , Zhou Wenke , Liu Zhendong , Wang Feng 
Published: September 2020

Abstract
Background: Kinesin family member 18A (KIF18A) belongs to a member of the kinesin superfamily and is significantly overexpressed and abnormally functions in a variety of cancers. However, the expression profiling and the role of KIF18A has so far remained unclear in glioma. To investigate the relationship of KIF18A with clinical practice and its role in gliomas, the data from multi public datasets were used and analyzed in present study.Methods: Patients with gliomas were enrolled from CGGA RNA-seq, TCGA-seq, CGGA-microarray, GSE4290 and GSE50161 databases. The expression of KIF18A in glioma and normal tissues was analyzed through Wilcoxon rank sum test, and the relationship between KIF18A and clinicopathological features was evaluated using logistic regression. The effect of KIF18A expression in the survival of glioma patients was explored by Kaplan-Meier and Cox analyses. Gene set enrichment analysis was also conducted to annotate biological function of KIF18A.Results: KIF18A expression was significantly increased in glioma tissues compared with the normal counterparts. High KIF18A expression shortened the overall survival in all grades gliomas and the survival period of patients with WHO grade Ⅲ glioma, but not significantly for grade Ⅱ and grade Ⅳ glioma. Univariate and multivariate analyses demonstrated that evaluated KIF18A expression is closely related to poor prognosis. Increased KIF18A expression was significantly correlated grades, age, IDH wide type and may serve as a biomarker of poor prognosis in gliomas. GSEA showed that the KEGG_DNA_REPLICATION, the KEGG_CELL_CYCLE, the KEGG_MISMATCH_REPAIR and the KEGG_NUCLEOTIDE_EXCISION_REPAIR were differentially enriched in KIF18A high expression phenotype.Conclusions: High expression KIF18A might be potential biomarker for the diagnosis and an independent risk factor for poor prognosis in glioma.
Aqp2 may affect the occurrence of acute kidney injury after cardiopulmonary bypass
Authors: Xiao Yingjie , Li Yanli , Dong Wenpeng , Gui Long , Yu Xu , Lin Min 
Published: September 2020

Abstract
BACKGROUND

Acute kidney injury (AKI) is one of the most important postoperative complications of cardiac surgery, and despite complete recovery of renal function after surgery, AKI is independently associated with high mortality within 10 years after surgery. Experiments show that Aquaporin 2(AQP2) is associated with postoperative AKI. Results of AQP2 release patterns after acute kidney injury have not been the same in different studies.
METHODS

We selected all patients undergoing extracorporeal circulation surgery, collected their urine samples before and after surgery, centrifuged at 3000 rpm for ten minutes, and detected the expression of AQP2 in urine by ELISA.
RESULTS

Most of the patients without AKI showed an upward trend of AQP2, while the patients with AKI showed a downward trend of AQP2. Patients with lower AQP2 levels before surgery have a lower chance of developing AKI.
CONCLUSION

AQP2 can be used to predict and prevent postoperative AKI.
Interference From Haematuria Renders the Urinary BTA Test Not Specific Enough for Diagnosing Bladder Cancer: Evidence From Clinical Practice
Authors: Xiong Xiong , He Wei , Xiong Bin , Yao Feng 
Published: September 2020

Abstract
Background The urinary bladder tumour antigen (BTA) stat test has already been used for the diagnosis and monitoring of bladder cancer (BC). However, more evidence is needed regarding its efficacy and utility in the clinic. In this study, we investigated the influence of haematuria on the performance of the BTA stat test in a clinical cohort.Methods Urine samples from 836 subjects, including 50 healthy volunteers, 553 patients with benign urologic disorders, 124 patients with histologically proven BC, and 109 patients with other histologically proven urologic cancers, were analysed by the BTA stat test and urinalysis. We detected the sensitivity and specificity of the BTA stat test in each group, and analysed the effect of haematuria on the specificity.Results Our data showed that 58.06% of patients had haematuria in the BC group. Haematuria with benign prostatic hyperplasia (BPH), renal hamartoma (RH) and urolithiasis were identified in 39.01%, 42.86% and 66.49% of patients with benign urologic disorders, respectively. Haematuria was identified in 48.72% of prostatic cancer patients and 67.74% of renal cancer patients. The overall sensitivity of the BTA stat test was 90.32%. The sensitivity was 97.22% in BC patients with haematuria and 80.77% in BC patients without haematuria. The overall specificity in healthy individuals, patients with benign urologic disorders and other urologic cancers was 50.84%. In all patients with haematuria, the specificity of the BTA stat test was 15.82%, while it was 72.6% in patients without haematuria.Conclusions Haematuria has a significant influence on the BTA stat test. Our study suggested that the BTA stat test is not an ideal diagnostic tool for BC.
Soluble advanced glycosylation receptor is a potential target for the treatment of neutrophilic asthma
Authors: Zhang Xiaobo , Xie Jun , Sun Hongmei , Wei Qing , Tao Ying , Nong Guangmin 
Published: September 2020

Abstract
Background

Neutrophilic asthma (NA) was a subtype of asthma. Soluble advanced glycosylation receptor (sRAGE) was considered to be associated with the neutrophilic airway. However, the role of sRAGE in NA still limited.
Methods

A NA mouse model was established and the levels of sRAGE in the bronchoalveolar lavage fluid (BALF) were measured by ELISA. Hematoxylin-eosin (HE) and Masson trichrome staining were used to identifying airway remodeling. Adeno-associated virus 9 (AAV9) overexpressed sRAGE and inhibitors for HMGB1, RAGE, and PI3K were used to intervene NA mouse model via tail-vein injection and intraperitoneally injection. Expressions of airway remodeling, EMT, and signaling markers were detected using qRT-PCR or western blotting. The levels of IL-17 and IL-6 in BALF were measured by ELISA. HMGB1 was applied to induce EMT of human bronchial epithelial cells (16HBE), then E-cadherin and vimentin expressions were examined after sRAGE, RAGE inhibitor, and PI3K inhibitor administration.
Results

sRAGE levels were significantly reduced in BALF and the airway remodeling was observed in the NA mouse model. AAV9-sRAGE significantly inhibited the neutrophilic airway inflammation, airway remodeling, and the expression of IL-17, IL-6, TGF-β1, RAGE, PI3K, and EMT markers -E-cadherin and vimentin in vivo. HMGB1 inhibitor, RAGE inhibitor, and PI3K inhibitor upregulated E-cadherin level. Moreover, HMGB1 promoted the EMT process via RAGE/PI3K in 16HBE cells and sRAGE reversed HMGB1- induced EMT in vitro.
Conclusion

sRAGE levels decrease in the mouse model with NA. sRAGE treatment attenuates neutrophilic airway inflammation, airway remodeling, and EMT. This suggests sRAGE may yield benefits in the treatment of NA.
Pain in Nursing Homes Residents With and Without Cognitive Impairment. Results From Five Consecutive Multicenter Cross-sectional Surveys Carried Out Between 2014 and 2018 in Germany
Authors: Centmayer Rainer , Leiske Manfred , Lahmann Nils Axel 
Published: September 2020

Abstract
Background: There is evidence that knowledge about the prevalence of pain and quality of pain management particularly in nursing home residents (NHR) with severe cognitive impairment (CI) is poor.Methods: The multicenter cross-sectional surveys explored the prevalence of pain of NHR with or without CI from nursing homes in Germany. Actual pain intensity in rest and stress were documented. NHR were asked about their daily restrictions due to pain. Data about the pain management were collected and analyzed.Results: A total of 3437 residents were interviewed with respect to feeling pain, including one third each with mild and severe CI. The prevalence of actual pain was 31,8%. Women reported pain more often. Prevalence of NHR without CI or with self-report on pain was significantly higher than NHR with severe CI or with external report on pain. About 20% of all NHR were dependent on external pain-recording. Nearly 10% of all NHR with pain confirmed pain in stress above 5 on a scale of 0 - 10. 85% of all NHR with pain reported that they had pain for longer than 3 months. Residents with severe CI are 0.55 times less likely to take painkillers than NHR without CI.Conclusion: The study points out a significant deficit in pain management in German NHR with severe CI. Intensive training in pain management for employees in nursing homes is recommended.
Nucleolin rescues TDP-43 toxicity in yeast and human cell models
Authors: Peggion Caterina , Massimino Maria Lina , Stella Roberto , Bortolotto Raissa , Agostini Jessica , Maldi Arianna , Sartori Geppo , Tonello Fiorella , Bertoli Alessandro , LOPREIATO RAFFAELE 
Published: September 2020

Abstract
Background

TDP-43 is a nuclear protein involved in pivotal processes, extensively studied for its implication in neurodegenerative disorders. TDP-43 cytosolic inclusions are a common neuropathologic hallmark in amyotrophic lateral sclerosis (ALS) and related diseases, and it is now established that TDP-43 misfolding and aggregation play a key role in their etiopathology. TDP-43 neurotoxic mechanisms are not yet clarified, but the identification of proteins able to modulate TDP-43-mediated damage may provide crucial information to unveil the molecular basis of TDP-43 proteinopathies.
Methods

Here we generated and characterized novel models of TDP-43 toxicity in the yeast S. cerevisiae, which were used to investigate the effect of the nucleolar protein nucleolin (NCL) on TDP-43-damaged yeast cells, by employing multiple approaches (genetics, biochemistry, microscopy). We further characterized the NCL-TDP-43 relationship in human HEK293T cells, by the combination of biochemical and microscopy-based assays.
Results

We show for the first time that NCL acts as a potent suppressor of TDP-43 toxicity in yeast models, since NCL overexpression is able to rescue TDP-43-dependent damage on cell viability and morphology, by reducing the levels of TDP-43 aggregates, thus proteostatic stress. Interestingly, data in yeast cells point to the implication of the extra-nuclear fraction of NCL in the suppressive effect. We further provide evidence that NCL co-expression alleviates the TDP-43-induced toxicity also in HEK293T cells, as indicated by the restoration of cell viability, and the diminished apoptosis activation. Importantly, biochemical and microscopy data indicate that NCL protein in human cells reduces the amount of TDP-43 inclusions. Collectively, results in HEK293T cells further support the beneficial effects of NCL on TDP-43-dependent toxicity in a more consistent pathophysiological context.
Conclusions

Altogether, data in yeast and human cell models demonstrate that NCL potently supresses the cytotoxicity caused by the TDP-43 protein, and further suggest that NCL could act by promoting the TDP-43 nuclear retention, and thus reducing the formation of cytosolic TDP-43 toxic aggregates. Pinpointing NCL as a novel player in mediating TDP-43 toxicity, experimental evidence could support NCL as promising therapeutic target in ALS and ALS-related disorders.
Effect of Poverty Volatility on Tax Revenue Instability in Developing Countries
Authors: GNANGNON SENA KIMM 
Published: September 2020

Abstract
This paper complements the relatively few existing studies on the macroeconomic effects of poverty in developing countries, by investigating the effect of poverty volatility on tax revenue instability. The empirical analysis has been conducted using an unbalanced panel dataset of 112 developing countries covering the period 1980-2017, and primarily the two-step system Generalized Method of Moments technique. Findings have revealed that, on average, over the full sample, poverty volatility is associated with lower tax revenue instability. However, this reflects differentiated effect across countries, as low-income countries tend to experience a positive tax revenue instability effect of poverty volatility, while poverty volatility results in lower tax revenue instability in relatively advanced countries (among developing countries). Additionally, these outcomes hide the fact that poverty volatility exerts a higher positive effect on tax revenue instability in the context of increasing poverty rates. From a policy perspective, this analysis shows that it is essential for policymakers to dampen the volatility of poverty rates (notably in countries with high poverty rates) if they were to ensure the stability of tax revenue or reduce its instability, given the adverse effect of tax revenue instability on economic growth.
Modulation of Visceral Sensitivity by Faecal Microbiota Transplantation (FMT): The Active Role of Gut Microbiota in the Persistence of Abdominal Pain
Authors: Lucarini Elena , Pilato Vincenzo Di , Parisio Carmen , Micheli Laura , Toti Alessandra , Pacini Alessandra , Bartolucci Gianluca , Baldi Simone , Niccolai Elena , Amedei Amedeo , Rossolini Gian M. , Nicoletti Claudio , Cryan John F. , O'Mahony Siobhain M. , Ghelardini Carla , Mannelli Lorenzo Di Cesare 
Published: September 2020

Abstract
Background: Recent findings linked gastrointestinal disorders characterized by abdominal pain to gut microbiota composition. The present work aimed to evaluate the power of gut microbiota as a visceral pain modulator and, consequently, the relevance of its manipulation as a therapeutic option in reversing the persistence of visceral hypersensitivity consequent to colitis induced by the intra-rectal injection of 2,4-dinitrobezenesulfonic acid (DNBS) in rats. Results: The effect of faecal microbiota transfer (FMT) from viscerally hypersensitive DNBS and naïve donors was evaluated in control rats after an antibiotic-mediated microbiota depletion. FMT from DNBS donors induced a long-lasting visceral hypersensitivity in control rats. Pain threshold trend correlated with major modifications in the composition and structure of the gut microbiota at phylum (Proteobacteria and Firmicutes to Bacteroides ratio) and family levels (Enterobacteriaceae, Akkermansiaceae and Lachnospiraceae). Acetic acid was significantly increased in the recipients FMT from DNBS donors. Gut cytokine profile, as well as tryptophan metabolism were similarly altered after FMT from both DNBS and naïve donors. By contrast, no significant alterations of colon histology, permeability and monoamines levels were detected. Finally, following FMT from healthy donors to DNBS-treated animals, a counteraction of persistent visceral pain was achieved. Conclusions: The present results provide novel insights into the relationship between intestinal microbiota and visceral hypersensitivity, highlighting the therapeutic potential of microbiota modulation on persistent abdominal pain.
Multiple sulfur isotopic reservoirs in the Moon and implications for the evolution of planetary interiors
Authors: Faircloth Samantha , Anand Mahesh , Franchi Ian , Zhao Xuchao , Russell Sara 
Published: September 2020

Abstract
Very few in situ lunar sulfur studies exist, with the major focus being on bulk-rocks in which a relatively restricted sulfur isotope fractionation is observed, leading to suggestions that the source of sulfur in the lunar interior is homogeneous. Using a novel approach, we present for the first time two complementary datasets combining in situ secondary ion mass spectrometry and X-ray absorption near-edge structure spectroscopy of lunar apatite, to investigate the late-stage behaviour of sulfur in lunar basaltic melts. Our measurements reveal varied sulfur contents of ~20–2,800 ppm and δ34S values of -33.3 ± 3.8‰ to +36.4 ± 3.2‰ (2σ). The apatites have S6+/ΣStot ratios of >0, with average values as high as 0.55, providing evidence for the existence of relatively oxidized late-stage silicate melts on the Moon. We propose the existence of multiple, previously unrecognised, distinct sulfur isotopic reservoirs in the lunar interior and atypical oxidizing conditions in late-stage silicate melts. These findings are important for our understanding of lunar formation processes and the evolution of redox conditions during the formation of terrestrial bodies.
Correlations of PD-1/PD-L1 Gene Polymorphisms with Susceptibility and Prognosis in Non-Hodgkin lymphoma in Iranian Population
Authors: hoseini hosnie , Yaghmaei Parichehreh , Bahari Gholamreza , aminzadeh saeed 
Published: September 2020

Abstract
Background,

Programed cell death 1 (PD-1) and its ligand (PD-L1) activity have already detected in various cancers. In non-Hodgkin lymphoma (NHL), however, the prognostic value of PD-1/PD-L1 genes polymorphisms and expression levels remains unclear. In the present study we aimed to investigate the relationship between genetic polymorphisms of PD-1/PD-L1 genes and non-Hodgkin lymphoma in Iranian papulation.

Methods

, four single nucleotide polymorphisms of the PD-1/PD-L1 genes, including 134 NHL patients and 125 healthy controls were examined using PCR-RFLP method. The expression level of PD-1/PD-L1 genes were analyzed using real time PCR method.

Results

, our data demonstrated that the PD-L1 rs2890685 (A>C) SNP (p<0.0001) significantly was associated with increased risk of NHL. The AA genotype of PD-L1 rs2890685 polymorphism was found to be more prevalent in NHL patents compared to healthy controls. No significant association were found between PD-L1 rs4143815, PD-1 rs11568821, PD-1rs2227981 SNPs and the risk of NHL incidence. Furthermore, our data showed that the mRNA transcription levels of both PD-1 and PD-L1 were significantly higher than normal healthy controls (p<0.001).

Conclusion

, Collectively, our finding demonstrated that the functional PD-L1 rs2890685 polymorphism was associated with NHL risk, suggesting that genetic variant of PD-L1 might be a possible prognosis marker for prediction of NHL risk and its development.
Evaluating Context-Specific Evidence-Based Quality Improvement Intervention on Lymphatic Filariasis Mass Drug Administration in Northern Ghana Using the RE-AIM Framework.
Authors: Manyeh Alfred Kwesi , Chirwa Tobias , Ramaswamy Rohit , Baiden Frank , Ibisomi Latifat 
Published: September 2020

Abstract
BackgroundOver a decade of implementing a global strategy to eliminate lymphatic filariasis in Ghana through mass drug administration, the disease is still being transmitted in 22 districts out of an initial 98 endemic districts identified in 2000. A context-specific evidence-based quality improvement intervention was implemented in the Bole District of Northern Ghana after an initial needs assessment. This study aimed to evaluate the process and impact of the intervention on implementation of lymphatic filariasis mass drug administration in Bole District of Northern Ghana. MethodThe study used the RE-AIM (Reach, Effectiveness, Adoption, Implementation, and Maintenance) framework based on a mixed-methods approach to evaluate the context-specific evidence-based quality improvement intervention. Results The findings of the evaluation showed that there was an improvement in social mobilization and sensitization, knowledge about lymphatic filariasis and mass drug administration process, willingness to ingest the medication, and adherence to the directly observe treatment strategy. We observed a 6.3% increase in the district mass drug administration coverage and reduction in self-reported adverse drug reaction. The level of reach, effectiveness and adoption as the district, sub-district and at the individual participants’ level suggest that the context-specific evidence-based quality improvement intervention is feasible to implement in LF hotspot districts based on initial context-specific needs assessment. ConclusionThe study provided the groundwork for future application of the RE-AIM framework to evaluate the implementation of context-specific evidence-based quality improvement intervention to improve mass drug administration towards the elimination of lymphatic filariasis as a public health problem.
Postpartum depressive symptoms during the COVID-19 pandemic in Japan: The first quantitative evidence
Authors: Matsushima Midori , Horiguchi Hanna 
Published: September 2020

Abstract
Purpose: This study explores postpartum depression during the COVID-19 pandemic in Japan.Methods: An online survey was conducted from May 31 to June 6, 2020 and obtained 2,878 responses from mothers with infants less than 12 months. Results: The point prevalence of the Edinburgh Postnatal Depression Scale score of ≥ 9 was 26.96%. Logistic regression analysis indicated a negative association between the COVID-19 pandemic and mental well-being. Conclusion: In particular, our results indicate the importance of social support and economic recovery in ameliorating mental health of mothers in the postpartum period.
Cost-Effectiveness Analysis of A Quality Improvement Program to Reduce Cesarean Sections in Brazilian Private Hospitals: A Case Study
Authors: Domingues Rosa Maria Soares Madeira , Luz Paula Mendes , Ayres Bárbara Vasques da Silva , Torres Jacqueline Alves , Leal Maria do Carmo 
Published: September 2020

Abstract
Background: In 2015, a quality improvement project of childbirth care called Adequate Birth Project (“Projeto Parto Adequado”- PPA) was implemented in Brazilian public and private hospitals, aiming to improve the quality of childbirth care and to reduce cesarean sections without clinical indications. The objective of this study is to conduct an economic analysis of two models of care existing in a private Brazilian hospital - the model following the recommendations of the PPA and the standard of care model - in reducing the proportion of cesarean sections. Methods: We conducted a case study in one of the private hospitals included in the PPA project. The main outcome was the proportion of cesarean section. We used total cost of hospitalization for women and newborns, from the perspective of the health care provider, during the length of the observed hospital stay. We did not apply discount rates and inflation rate adjustments due to the short time horizon. We conducted univariate sensitivity analysis using the minimum and maximum costs observed in hospitalizations and variation in the probabilities of cesarean section and of maternal and neonatal complications. Results: 238 puerperal women were included in this analysis. The PPA model of care resulted in a 56.9 percentage point reduction in the cesarean section probability (88.6% vs 31.7%, p <0.001) and an increase in the total cost of US$ 67,346.25, which an incremental cost-effectiveness ratio of US$ 1,183.59 per avoided cesarean section. Women in the PPA model of care also had a higher proportion of spontaneous and induced labor and a lower proportion of early term births. There were no maternal, fetal or neonatal deaths and no significant differences in cases of maternal and neonatal nearmiss. The cost of uncomplicated vaginal births and cesarean sections was the parameter with the greatest impact on the cost-effectiveness ratio of the PPA model of care. Conclusion: The quality improvement project of childbirth care “PPA” was cost-effective in reducing cesarean sections in women assisted in a Brazilian private hospital, without increasing severe negative maternal and neonatal outcomes and reducing the frequency of early term births.