bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pedagogia em Performance: Uma abordagem do ensino do teatro na escola básica
Authors: Martins Pedro Haddad 
Published: December 2019

O presente artigo busca contextualizar, articulando teoria e prática no dia-a-dia da sala de aula, o que seria uma pedagogia em performance – um ensino do teatro que se afirma como prática contemporânea a partir do teatro performativo, e não esquece elementos de sua tradição. Aqui, a performatividade da criança é tomada como material poético para a construção teatral, e efetiva a experiência como prática de elaboração do conhecimento em arte. A pesquisa, realizada com 38 turmas de teatro do sexto ano do ensino básico entre 2012 e 2017, procura, através do relato de experiências, uma possível resposta para a pergunta: quais abordagens do ensino/aprendizagem do teatro são potentes hoje?
Para pensar a escola como espaço teatral: o teatro como ação tática no/do cotidiano
Authors: Silva Renata Patrícia 
Published: December 2019

Neste artigo propõe-se a discussão do teatro como ação tática no/do cotidiano da escola, a fim de contribuir para que ela seja um lugar praticado pelo fazer teatral. Para tanto, parte-se da compreensão de que a escola é um lugar que abriga práticas formalizadas e saberes-fazeres cotidianos. Diante disso, aponta-se que o teatro, se insere dentro das formalidades institucionais e dos fazeres ordinários, ponderando sua condição de prática formalizada, que se (re)inventa a partir das ações dos sujeitos no/do cotidiano escolar. Desta forma, ao discorrer sobre a docência como ação tática, compartilha-se a proposta de ações com o teatro na escola, que atuaram enquanto micropolítica cotidiana, provocando pequenas rupturas e movimentos de (re)existência, apropriando-se do território das formalidades da educação institucionalizada, a fim de legitimar o lugar da arte dentro da escola e criar espaços outros.
O grotesco, a commedia dell’arte e o clown: visão de dentro e de fora
Authors: Araújo Raíssa Guimarães de Souza , Sperber Suzi 
Published: December 2019

As linguagens da commedia dell’arte e do clown, em diálogo com o conceito de grotesco, no Barracão Teatro (Campinas - SP). Parte-se das observações e vivências destas linguagens - e das entrevistas com Tiche Vianna e Ésio Magalhães, que abordam especialmente a montagem da comédia “Zabobrim: O rei vagabundo” - para as influências e atravessamentos no trabalho de uma das autoras enquanto atriz, palhaça e pesquisadora da comicidade de cunho popular. Conclusão da análise da linguagem do cômico do Barracão Teatro feito por receptora, i.e., público - e outra autora do presente artigo.
Benefits of Multiple Sclerosis Adult Day Program Participation for People with Multiple Sclerosis
Authors: Marrow Jocelyn , Roeser Allison , Gasper Joseph , LaRocca Nicolas G. , Frankel Debra 
Published: December 2019

Abstract

Background:
Multiple sclerosis adult day programs (MSADPs) are nonmedical service programs that provide care and support to people with multiple sclerosis (MS) experiencing levels of impairment that interfere with employment and activities of daily living. The purpose of this study was to identify how program participants think MSADPs benefit them.

Methods:
We conducted in-depth interviews with 95 people with MS who were MSADP participants. Interviews occurred as part of site visits to ten programs across the country. We supplemented participant interviews with interviews of program staff and ethnographic observation of activities at each site over 1 to 3 days.

Results:
Participants reported several benefits from participating in an MSADP. Almost universally, participants explained that receiving and giving social support were the most important benefits. Other positive outcomes described included increased emotional well-being, better understanding and acceptance of MS, practical know-how for accomplishing ordinary tasks, and increased mobility and improved motor functioning.

Conclusions:
Participants believe that MSADPs have a profound influence on their psychosocial health, much of which they attribute to membership in a socially cohesive community. Clinicians and policy makers should consider recommending these programs for people with MS who have unmet psychosocial needs and experience functional impairments.
Studying the impact of CI on pull request delivery time in open source projects—a conceptual replication
Authors: Guo Yunfang , Leitner Philipp 
Published: December 2019

Nowadays, continuous integration (CI) is indispensable in the software development process. A central promise of adopting CI is that new features or bug fixes can be delivered more quickly. A recent repository mining study by Bernardo, da Costa & Kulesza (2018) found that only about half of the investigated open source projects actually deliver pull requests (PR) faster after adopting CI, with small effect sizes. However, there are some concerns regarding the methodology used by Bernardo et al., which may potentially limit the trustworthiness of this finding. Particularly, they do not explicitly control for normal changes in the pull request delivery time during a project’s lifetime (independently of CI introduction). Hence, in our work, we conduct a conceptual replication of this study. In a first step, we replicate their study results using the same subjects and methodology. In a second step, we address the same core research question using an adapted methodology. We use a different statistical method (regression discontinuity design, RDD) that is more robust towards the confounding factor of projects potentially getting faster in delivering PRs over time naturally, and we introduce a control group of comparable projects that never applied CI. Finally, we also evaluate the generalizability of the original findings on a set of new open source projects sampled using the same methodology. We find that the results of the study by Bernardo et al. largely hold in our replication. Using RDD, we do not find robust evidence of projects getting faster at delivering PRs without CI, and we similarly do not see a speed-up in our control group that never introduced CI. Further, results obtained from a newly mined set of projects are comparable to the original findings. In conclusion, we consider the replication successful.
A novel approach to immunoapheresis of C3a/C3 and proteomic identification of associates
Authors: Winnicki Wolfgang , Pichler Peter , Mechtler Karl , Imre Richard , Steinmacher Ines , Sengölge Gürkan , Knafl Daniela , Beilhack Georg , Wagner Ludwig 
Published: December 2019

BackgroundComplement factor C3 represents the central component of the complement cascade and its activation split product C3a plays an important role in inflammation and disease. Many human disorders are linked to dysregulation of the complement system and alteration in interaction molecules. Therefore, various therapeutic approaches to act on the complement system have been initiated.Methods and ResultsAiming to develop a tool to eliminate C3a/C3 from the circulation, in a first step a high affine murine monoclonal antibody (mAb) (3F7E2-mAb) was generated against complement factor C3 and selected for binding to the C3a region to serve as immunoaffinity reagent. Functional testing of the 3F7E2-mAb revealed an inhibition of Zymosan-induced cleavage of C3a from C3. Subsequently, a C3a/C3 specific 3F7E2-immunoaffinity column was developed and apheresis of C3a/C3 and associates was performed. Finally, a proteomic analysis was carried out for identification of apheresis products. C3a/C3 was liberated from the 3F7E2-column together with 278 proteins. C3a/C3 interaction specificity was validated by using a haptoglobin immunoaffinity column as control and biostatistic analysis revealed 39 true C3a/C3 interactants.ConclusionA novel and functionally active mAb was developed against complement factor C3a/C3 and used in a specific immunoaffinity column that allows apheresis of C3a/C3 and associates and their identification by proteomic analysis. This methodological approach of developing specific antibodies that can be used as immunoaffinity reagents to design immunoaffinity columns for elimination and further identification of associated proteins could open new avenues for the development of tailored immunotherapy in various complement-mediated or autoimmune diseases.
Algorithms for efficiently collapsing reads with Unique Molecular Identifiers
Authors: Liu Daniel 
Published: December 2019

Background
Unique Molecular Identifiers (UMI) are used in many experiments to find and remove PCR duplicates. There are many tools for solving the problem of deduplicating reads based on their finding reads with the same alignment coordinates and UMIs. However, many tools either cannot handle substitution errors, or require expensive pairwise UMI comparisons that do not efficiently scale to larger datasets.

Results
We reformulate the problem of deduplicating UMIs in a manner that enables optimizations to be made, and more efficient data structures to be used. We implement our data structures and optimizations in a tool called UMICollapse, which is able to deduplicate over one million unique UMIs of length 9 at a single alignment position in around 26 s, using only a single thread and much less than 10 GB of memory.

Conclusions
We present a new formulation of the UMI deduplication problem, and show that it can be solved faster, with more sophisticated data structures.
Decellularization Techniques for Preparation of Decellularized Extracellular Matrices in Tissue Engineering Applications
Authors: Chen Guoping , Kawazoe Naoki 
Published: December 2019

Abstract
Decellularized extracellular matrix (ECM) scaffolds have been broadly used in tissue engineering because of their versatile bioactive nature and biomimetic properties. The ECM can be derived from various tissues, organs and cultured cells. A variety of decellularization methods have been developed to maximize the decellularization effect while minimizing the effect on ECM structures and compositions. The methods can be categorized into chemical, biological, and physical methods and their combinations. The properties and applications of ECM scaffolds are dependent on decellularization methods. This article summarizes the decellularization methods for preparation of decellularized ECM scaffolds for tissue engineering applications.
Determination of Validation Errors Precisely Using Bootstrapped Latin Partitions
Authors: de Boves Harrington Peter 
Published: December 2019

AbstractA history of nonparametric and distribution‐free validation methods is presented. Historically, only single measures of error have been used for chemometric model optimization and validation. With the advent of modern personal computing, multiple validation methods become realizable using resampling techniques such as bootstrapping. However, bootstrapping requires random sampling of the data objects, so some sophisticated splitting methods are not amenable to bootstrapping. Furthermore, methods such as computer‐aided design of experiments (CADEX) (aka Kennard–Stone) provide biased estimates of the measured error. The Latin partition method constrains random splitting so that the class or property distribution is maintained between the splits of the data. Bootstrap analysis combined with Latin partitions has had a successful history for validating chemometric methods and developing self‐optimized chemometric methods that use the procedure internally. Several demonstrations with synthetic data reveal the advantages of the bootstrapped Latin partition method.
The Role of the Phyllosphere Microbiome in Plant Health and Function
Authors: Stone Bram W. G. , Weingarten Eric A. , Jackson Colin R. 
Published: December 2019

AbstractThe above‐ground surfaces of plants (the phyllosphere) harbour a diverse variety of microorganisms, and this phyllosphere microbiome interacts with the host plant affecting its health and function. Phyllosphere microorganisms, predominantly bacteria and fungi, can act as mutualists promoting plant growth and tolerance of environmental stressors, commensals using the leaf habitat for their own growth and reproduction, or as antagonistic pathogens. Although much of the literature has focused on plant–pathogen interactions and disease mechanisms, we expand this discussion to the structural and functional dynamics of the whole phyllosphere community, rather than single‐species populations. We highlight the particular challenges posed to microorganisms living on the leaf surface, describe the structure of the phyllosphere microbiome, and discuss how these microbial constituents interact with the plant with respect to stress tolerance, growth promotion, nutrient acquisition, and disease resistance. Lastly, we pose new directions for the field made possible by advances in sequencing and computational technologies.
High‐Performance Ionic‐Polymer–Metal Composite: Toward Large‐Deformation Fast‐Response Artificial Muscles
Authors: Ma Suqian , Zhang Yunpeng , Liang Yunhong , Ren Lei , Tian Wenjing , Ren Luquan 
Published: December 2019

AbstractAs promising candidates in the field of artificial muscles, ionic‐polymer–metal composites (IPMCs) still cannot simultaneously provide large deformations and fast responses, which has limited their practical applications. In this study, to overcome this issue, a Nafion‐based IPMC with high‐quality metal electrodes is fabricated via novel isopropanol‐assisted electroless plating. The IPMC exhibits a large tip displacement (35.3 mm, 102.3°) under a low direct‐current driving voltage and ultrafast response (>10 Hz) under an alternating‐current (AC) voltage. Furthermore, the simultaneous integration of a large deformation and fast response can be achieved by the IPMC under a high‐frequency (19 Hz) AC voltage, where the largest bending amplitude is 5.9 mm and the highest bending speed reaches 224.2 mm s−1 (596.2° s−1). Additionally, the lightweight IPMC exhibits a decent load capacity and can lift objects 20 times heavier. The outstanding performances of the Nafion IPMC are demonstrated by mimicking biological motions such as petal opening/closing, tendril coiling/uncoiling, and high‐frequency wing flapping. This study paves the way for the fabrication of lightweight actuators with simultaneous large displacements and fast responses for promising applications in biomedical devices and bioinspired robotics.
Low‐Operating Temperature, High‐Rate and Durable Solid‐State Sodium‐Ion Battery Based on Polymer Electrolyte and Prussian Blue Cathode
Authors: Du Guangyuan , Tao Mengli , Li Jie , Yang Tingting , Gao Wei , Deng Jianhua , Qi Yuruo , Bao Shu‐Juan , Xu Maowen 
Published: December 2019

AbstractA solid‐state polymer electrolyte (PFSA‐Na membrane) for solid‐state sodium‐ion batteries (SSIBs) to overcome severe safety issues caused by traditional liquid electrolytes is explored. The PFSA‐Na membranes, synthesized by an environmentally and economically friendly method, display high ionic conductivity, excellent thermal stability, and outstanding mechanical flexibility in a wide temperature range. SSIBs based on the PFSA‐Na membranes and Prussian blue cathode exhibit a superior rate performance of 87.5 mA h g−1 at 8 C and a durable cycling life of up to 1100 cycles at 1 C with only a slight capacity decay of ≈0.014% per cycle. Furthermore, due to the intrinsic advantages of the PFSA‐Na membranes, the cyclic performance of the proposed SSIBs is more stable than that of its liquid counterpart even at the rather low temperature of −35 °C.
Evaluation of the relationships of the WBP1L gene with schizophrenia and the general psychopathology scale based on a case–control study
Authors: Guan Fanglin , Ni Tong , Han Wei , Lin Huali , Zhang Bo , Chen Gang , Zhu Li , Liu Dan , Zhang Tianxiao 
Published: December 2019

AbstractWBP1L is a target of microRNA 137 (miR‐137) and has been considered a candidate gene for schizophrenia (SCZ). To investigate the relationships between WBP1L and SCZ and its related symptom scales, a total of 5,993 Chinese Han subjects, including 2,128 SCZ patients and 3,865 controls, were enrolled. In addition, an independent sample set for replication study including 1,052 SCZ patients and 2,124 controls were also recruited. Thirty‐two tag single nucleotide polymorphisms (SNPs) located within gene region of WBP1L were selected for genotyping and analyzing. The expression quantitative trait loci (eQTL) effects for the targeted SNPs were investigated with gene expression data from multiple human tissues. Rs4147157 (OR = 0.84, p = 1.51 × 10−5) and rs284854 (OR = 1.14, p = 7.00 × 10−4) were significantly associated with SCZ disease status and these association signals were replicated in our replication sample. A significant association was identified between rs4147157 and the general (β = −.66, p = .001) and total (β = −.8, p = .0042) scores of positive and negative syndrome scale scores in SCZ patients. Both SNPs were significant eQTL for genes around WBP1L in human brain tissues including ARL3 and AS3MT. To conclude, SNPs rs4147157 and rs284854 were associated with SCZ in the Chinese Han population. Additionally, rs4147157 was significantly associated with specific symptom features of SCZ.
Regioselective Hydroiodination of Alkynes by in situ‐Generated HI
Authors: Li Jiayan , Devi Laishram Ronibala , Chen Jingchao , Xu Dandan , Shi Guangrui , Lv Haiping , Fan Ruifeng , Fan Baomin 
Published: December 2019

AbstractAn efficient and highly selective hydroiodination of alkynes has been reported. The reaction utilizes in situ‐generated HI from [Rh]‐complex, molecular hydrogen, and iodine under ambient temperature. The methodology provided the Markovnikov products exclusively from terminal alkynes, and predominately E‐isomers from the internal alkynes in good yields. The reaction could be performed on a gram‐scale with good yield.
Dibortetraiodid (B2I4) ist im Festkörper ein Polymer aus sp3‐hybridisiertem Bor
Authors: Muessig Jonas H. , Lisinetskaya Polina , Dewhurst Rian D. , Bertermann Rüdiger , Thaler Melanie , Mitrić Roland , Braunschweig Holger 
Published: December 2019

AbstractAnhand der ersten Festkörperstrukturen von Dibortetraiodid (B2I4) wird gezeigt, dass dieses nicht, wie lange angenommen, analog zu den leichteren Dibortetrahalogeniden B2F4, B2Cl4 und B2Br4 in allen Aggregatzuständen in Form diskreter Moleküle mit planaren, dreifach koordinierten Boratomen vorliegt. Röntgenstrukturanalysen, Festkörper‐NMR‐ und IR‐Messungen zeigen, dass B2I4 im Festkörper in zwei polymeren Konformeren vorkommt, die tetraedrisch koordinierte Boratome enthalten. Anhand von DFT‐Rechnungen werden die IR‐Spektren in Lösung und im Festkörper simuliert und mit den experimentellen Daten verglichen.
Preventing thermal degradation of PVC insulation by mixtures of cross‐linking agents and antioxidants
Authors: Brostow Witold , Hnatchuk Nathalie , Kim Taehwan 
Published: December 2019

ABSTRACTPoly(vinyl chloride) (PVC) is widely used—in spite of HCl formed from it at elevated temperatures. PVC wire and cable insulation has poor thermal stability, causing the plasticizer to separate from the PVC chains and produce an oily residue, lowering the tensile elongation at break and thus increasing brittleness. One uses cross‐linking agents and antioxidants, as well as mixtures of both, to improve the thermal stability of the plasticizer and tensile properties of PVC after thermal exposure. We performed tensile tests, tribological tests, profilometry, scanning electron microscopy (SEM), and water absorption determination before and after thermal exposure at 136 °C for 1 week. After adding the agents, elongation at break increased by 10 to 20% while the wear rate and water absorption were lower than for the control sample. Less voids are seen in the SEM images after adding these two kinds of agents. The thermal resistance of the PVC cable insulation is best enhanced by combinations of cross‐linking agents and antioxidants. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48816.
In‐depth characterization of genome‐scale network reconstructions for the in vitro synthesis in cell‐free systems
Authors: Schuh Lisa Katharina , Weyler Christian , Heinzle Elmar 
Published: December 2019

AbstractCell‐free systems containing multiple enzymes are becoming an increasingly interesting tool for one‐pot syntheses of biochemical compounds. To extensively explore the enormous wealth of enzymes in the biological space, we present methods for assembling and curing data from databases to apply them for the prediction of pathway candidates for directed enzymatic synthesis. We use Kyoto Encyclopedia of Genes and Genomes to establish single organism models and a pan‐organism model that is combining the available data from all organisms listed there. We introduce a filtering scheme to remove data that are not suitable, for example, generic metabolites and general reactions. In addition, a valid stoichiometry of reactions is required for acceptance. The networks created are analyzed by graph theoretical methods to identify a set of metabolites that are potentially reachable from a defined set of starting metabolites. Thus, metabolites not connected to such starting metabolites cannot be produced unless new starting metabolites or reactions are introduced. The network models also comprise stoichiometric and thermodynamic data that allow the definition of constraints to identify potential pathways. The resulting data can be directly applied using existing or future pathway finding tools.
ECM concentration and cell‐mediated traction forces play a role in vascular network assembly in 3D bioprinted tissue
Authors: Zhang Guangliang , Varkey Mathew , Wang Zhan , Xie Beibei , Hou Ruixing , Atala Anthony 
Published: December 2019

AbstractTissue vascularization is critical to enable oxygen and nutrient supply. Therefore, establishing expedient vasculature is necessary for the survival of tissue after transplantation. The use of biomechanical forces, such as cell‐induced traction forces, may be a promising method to encourage growth of the vascular network. Three‐dimensional (3D) bioprinting, which offers unprecedented versatility through precise control over spatial distribution and structure of tissue constructs, can be used to generate capillary‐like structures in vitro that would mimic microvessels. This study aimed to develop an in vitro, 3D bioprinted tissue model to study the effect of cellular forces on the spatial organization of vascular structures and tissue maturation. The developed in vitro model consists of a 3D bioprinted polycaprolactone (PCL) frame with a gelatin spacer hydrogel layer and a gelatin–fibrin–hyaluronic acid hydrogel layer containing normal human dermal fibroblasts and human umbilical vein endothelial cells printed as vessel lines on top. The formation of vessel‐like networks and vessel lumens in the 3D bioprinted in vitro model was assessed at different fibrinogen concentrations with and without inhibitors of cell‐mediated traction forces. Constructs containing 5 mg/ml fibrinogen had longer vessels compared to the other concentrations of fibrinogen used. Also, for all concentrations of fibrinogen used, most of the vessel‐like structures grew parallel to the direction the PCL frame‐mediated tensile forces, with very few branching structures observed. Treatment of the 3D bioprinted constructs with traction inhibitors resulted in a significant reduction in length of vessel‐like networks. The 3D bioprinted constructs also had better lumen formation, increased collagen deposition, more elaborate actin networks, and well‐aligned matrix fibers due to the increased cell‐mediated traction forces present compared to the non‐anchored, floating control constructs. This study showed that cell traction forces from the actomyosin complex are critical for vascular network assembly in 3D bioprinted tissue. Strategies involving the use of cell‐mediated traction forces may be promising for the development of bioprinting approaches for fabrication of vascularized tissue constructs.