bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation and Characterization of Injectable Augmentation Gels Containing Polycaprolactone and Hyaluronic Acid
Authors: Turkmen Meryem Erken , Ghaffarlou Mohammadreza , Kilic Busra , Karaaslan Cagatay , Aydin Halil Murat 
Published: December 2024

ABSTRACTBackgroundInjectable augmentation gels are widely used in the treatment of soft tissue. The composition of these gels has to be continuously improved due to the limitations of the currently available formulations.AimsThis study focuses on the development of an innovative injectable gel designed to address current trends and specific needs within the field.MethodsThe current study utilized a safer hyaluronic acid (HA) gel carrier, created with a less toxic cross‐linker, in combination with polycaprolactone (PCL) microspheres at various concentrations. PCL microspheres were prepared using an emulsification‐solvent evaporation technique. Six different gel formulations were developed using PCL microspheres and biphasic HA gel structures.ResultsThe produced microspheres had non‐agglomerated, smooth surfaces with an average particle size of approximately 45 ± 0.14 μm. The rheological results of the PCL‐HA6 such as storage modulus (G', Pa), loss modulus (G", Pa), complex viscosity (η*), and phase angle (°) at 1 Hz frequency were measured as 553.97 ± 32.48, 368.4 ± 12.24, 105.9 ± 5.27, and 33.65 ± 0.92, respectively, and the injection force was measured as 9.64 ± 1.46. In vitro tests revealed that the PCL‐HA6 group showed the highest cell viability compared to the other groups and provided a relative increase in collagen production over time, as demonstrated by the relevant gene expression.ConclusionsThe developed PCL/HA gel exhibited biocompatibility and non‐toxicity, making it a safe option for soft tissue augmentation. It demonstrated potential for medical applications and exhibited favorable rheological characteristics and injectability.
The Role of Patient‐ and Drug‐Related Factors in Oral Minoxidil and Pericardial Effusion: Analyses of Data From the United States Food and Drug Administration Adverse Event Reporting System
Authors: Gupta Aditya K. , Bamimore Mary A. , Haber Robert , Williams Greg , Piguet Vincent , Talukder Mesbah 
Published: December 2024

ABSTRACTBackgroundWhile oral minoxidil (OM) has been associated with pericardial effusion (PE), its etiology is presently inconclusive.AimsWe characterized patient‐ and drug‐related factors across reports from the United States Food and Drug Administration Adverse Event Reporting System (FAERS) for PE and OM.MethodsOur observation period spanned 18.5 years. Parametric and non‐parametric analyses were used; we stratified our findings according to two groups of adverse events (AEs), namely, PE and all other AEs.ResultsAcross reports of OM (n = 2747), positive dechallenge (complete resolution or subsiding of AE upon discontinuation of OM) was significantly more likely to occur for PE than for all other AEs (p < 0.05). Furthermore, OM was significantly more likely to play a primary role in PE compared to all other AEs (p < 0.05). The proportion of men was significantly higher in OM reports of PE than in OM reports of all other AEs (p < 0.05). We also identified six reports of PE and topical minoxidil.ConclusionsThough findings from spontaneously reported data never prove causality, our findings on dechallenge and purported role may suggest one. There were no reports of PE at a dose < 2.5 mg/day, 2/35 reports at 2.5 mg/day, and 8/35 reports at 5 mg/day. Overall, the results of statistical analyses support that the relationship between OM and PE is dose independent. Caution should also be taken when applying minoxidil topically because of reports of PE associated with this route of administration.
The effects of age and sex on reference intervals for cobalamin, homocysteine, and serum and urinary methylmalonic acid in healthy adult dogs
Authors: Proksch Anna‐Lena , Schaefer Sophia , Dreller Vanessa , Langenstein Judith , Fingerhut Ralph , Bauer Natali , Moritz Andreas 
Published: December 2024

AbstractBackgroundIn dogs, data on reference intervals (RIs) for cobalamin, markers of metabolism (markersB12met), age and sex effects are limited.Hypothesis/ObjectivesEstablish RI for serum cobalamin, homocysteine, and methylmalonic acid (sMMA) concentrations, urinary methylmalonic acid‐to‐creatinine ratio (uMMA:crea), and determine effects of sex and age.MethodsProspective study using healthy dogs (1‐10 years). Cobalamin and markersB12met were determined using chemiluminescence immunoassay (cobalamin) and liquid chromatography/tandem mass spectrometry (homocysteine, sMMA, uMMA:crea). In dogs with outlying data, changes in health, markersB12met, and onset of gastrointestinal signs were reevaluated after 9‐15 months.ResultsTwelve of 120 healthy dogs had abnormal uMMA:crea ratios. No other cobalamin analyte outliers were found. Outlying data re‐examination (odRE) was performed in 10/12 dogs. Chronic gastrointestinal signs occurred in 64% of odRE‐dogs, whereas 36% remained healthy. In total, 112 dogs (67 females, 45 males; median ages, 3.5 and 3.75 years, respectively) were included in RI analyses. Reference intervals were 178.5‐851 pmol/L (cobalamin), 5.8‐29.0 μmol/L (homocysteine), 45.3‐159.5 μg/L (sMMA), and ≤22.4 mg/g (uMMA:crea). Only age affected cobalamin concentrations (significant decrease). Compared by sex and neuter status, intact male dogs had significantly higher uMMA:crea ratios (median, 13.5; range, 1.9‐83.6 mg/g) than the other groups (median, 2.5; range, 0.7‐9.7 mg/g; P < .0001). Sex‐specific RI were ≤58.9 mg/g (intact male) vs ≤5.2 mg/g (females and neutered males).Conclusion and Clinical ImportanceIntact male dogs had significantly higher uMMA:crea ratios than the other groups. Thus, sex‐specific RI are recommended for uMMA:crea. Because of the wide distribution of uMMA:crea ratios, careful interpretation in intact male dogs is advised.
Implementing Equality: State (Non)compliance With Judicial Revisions to Public Policy on Gay Rights
Authors: Peterson Jordan Carr 
Published: December 2024

ABSTRACTBecause the civil rights movement accomplished many successes via litigation, advocacy communities regard courts as bulwarks against the legal enshrinement of oppression targeting vulnerable minorities. Advocates of LGBTQ equality are no different, identifying the judiciary as critical to eradicating discrimination on the basis of sexual orientation. Standing alone, however, Supreme Court holdings revising the scope of civil rights for sexual minorities represent a change in policy formulation rather than implementation. Judicially directed reformulations of public policy require cooperation by officials responsible for implementation, and compliance rates with judicial directives vary. Here, I analyze state responses to two Supreme Court decisions involving sexual minorities: Lawrence v. Texas, decriminalizing sodomy, and Obergefell v. Hodges, invalidating state same‐sex marriage bans. Although adherence to Obergefell was swift and nearly universal, compliance outcomes for Lawrence have been uneven, as some states continue to enforce statutes that are materially equivalent to the “homosexual conduct” law reviewed in Lawrence. I argue compliance with Lawrence has lagged compared to Obergefell for the following three reasons: (1) linguistic imprecision in Lawrence; (2) divergent views of the right(s) at issue in each case; and (3) the absence of a federal executive regime credibly committed to the case's outcome.
Phenotypic and Genomic Signatures of Latitudinal Local Adaptation Along With Prevailing Ocean Current in a Coastal Goby
Authors: Hirase Shotaro , Nagano Atsushi J. , Nohara Kenji , Kikuchi Kiyoshi , Kokita Tomoyuki 
Published: December 2024

ABSTRACTIn the marine realm, unidirectional ocean currents often lead to high migration rates of marine organisms and, therefore, inhibit the formation of their latitudinal genetic structure. In contrast, cryptic latitudinal structures associated with local adaptation may frequently exist in widespread species generally exposed to a strong environmental heterogeneity. However, our understanding of the evolvability of locally adapted populations in open marine environments still needs to be completed. The coastal area along the Sea of Japan, where the Tsushima Warm Current flows from south to north in the Japanese Archipelago, provides a good model system for exploring this question. This study explored evidence for latitudinal local adaptation along with the prevailing ocean current in the ice goby Leucopsarion petersii at the phenotypic and genomic levels. Common garden experiments clearly showed genetically based clinal variation in growth rate, strongly suggesting local adaptation through conutergradient selection of this fitness‐related trait. Analyses based on reduced‐representation sequencing revealed a slight signal of genetic differentiation between the southern and northern populations, although continuous historical gene flow between them was supported by demographic modelling. Also, whole‐genome resequencing showed their independent demographic history during the last glacial period. Thus, these results suggest that gene flow along with the prevailing ocean current is somewhat limited, and the populations are not completely panmictic. Furthermore, the selection scan based on low‐coverage genome‐wide sequencing detected putative genomic signatures of latitudinal adaptation of growth‐related genes. Thus, our integrative study provided a novel example of marine local adaptation under a large ocean current.
Two‐step rapid inspection of underwater concrete bridge structures combining sonar, camera, and deep learning
Authors: Sun Weihao , Hou Shitong , Wu Gang , Zhang Yujie , Zhao Luchang 
Published: December 2024

AbstractUnderwater defects in piers pose potential hazards to the safety and durability of river‐crossing bridges. The concealment and difficulty in detecting underwater defects often result in their oversight. Acoustic methods face challenges in directly achieving accurate measurements of underwater defects, while optical methods are time‐consuming. This study proposes a two‐step rapid inspection method for underwater concrete bridge piers by combining acoustics and optics. The first step combines macroscopic sonar scanning with an enhanced YOLOv7 to detect and locate piers and defects. Second, the camera approaches the defects for image acquisition, and an enhanced DeepLabv3+ is used for defect identification. The results demonstrate an average mean average precision@0.5 of 95.10% for defect and pier detection, and an mean intersection over union of 0.914 for exposed reinforcement and spalling identification. The method was applied to a real river‐crossing bridge and reduced inspection time by 51.2% compared to traditional methods for assessing a row of 11 piers.
Prevalence of interocular symmetry in corneal astigmatism and the possible influence of age, sex and refractive error
Authors: Salvador‐Roger Raquel , Micó Vicente , Esteve‐Taboada José J. 
Published: December 2024

AbstractPurposeThe aim of this study was to investigate, using a power vector approach, whether corneal astigmatism follows a mirror symmetry pattern considering both the magnitude and axis, and whether age, sex and spherical equivalent refractive error can influence the pattern.MethodsThe IOLMaster 700 optical biometer was used to measure the radii of curvature of the anterior corneal surface. Refractive error was determined by non‐cycloplegic subjective refraction. Descriptive statistical analyses and inferential logistic regression were applied over the dichotomous variable of mirror symmetry using J0 and J45 power vector components. An evaluation was carried out based on the subject's age, sex and spherical equivalent refractive error.ResultsA total of 2974 Caucasian adults were evaluated. This cross‐sectional study revealed that axis orientation follows the isorule symmetry pattern, and in terms of both magnitude and axis orientation, mirror symmetry was present in 70.9% of cases. Age, sex and spherical equivalent refractive error were not significant factors and did not contribute to the clinical improvement of the model despite its statistical significance (refractive error, p = 0.001; age and sex, p = 0.23 and 0.36, respectively).ConclusionsAmong an adult Caucasian population, the prevalence of corneal astigmatism mirror symmetry was 70.9% and isorule symmetry was the most common pattern considering axis orientation only. The inclusion of age, sex and spherical equivalent refractive error did not improve the model.
Diverse functional interactions between ABA and ethylene in plant development and responses to stress
Authors: Liu Xu‐Dong , Zeng Yuan‐Yuan , Hasan Md. Mahadi , Ghimire Shantwana , Jiang Hui , Qi Shi‐Hua , Tian Xue‐Qian , Fang Xiang‐Wen 
Published: December 2024

AbstractAbscisic acid (ABA) and ethylene are two essential hormones that play crucial roles throughout the entire plant life cycle and in their tolerance to abiotic or biotic stress. In recent decades, increasing research has revealed that, in addition to their individual roles, these two hormones are more likely to function through their interactions, forming a complex regulatory network. More importantly, their functions change and their interactions vary from synergistic to antagonistic depending on the specific plant organ and development stage, which is less focused, compared and systematically summarized. In this review, we first introduce the general synthesis and action signaling pathways of these two plant hormones individually and their interactions in relation to seed dormancy and germination, primary root growth, shoot development, fruit ripening, leaf senescence and abscission, and stomatal movement regulation under both normal and stress conditions. A better understanding of the complex interactions between ABA and ethylene will enhance our knowledge of how plant hormones regulate development and respond to stress and may facilitate the development of crops with higher yields and greater tolerance to stressful environments through tissue‐specific genetic modifications in the future.
Cannabis Expression Atlas: a comprehensive resource for integrative analysis of Cannabis sativa L. gene expression
Authors: Barbosa‐Xavier Kevelin , Pedrosa‐Silva Francisnei , Almeida‐Silva Fabricio , Venancio Thiago M. 
Published: December 2024

AbstractCannabis sativa L., a plant originating from Central Asia, is a versatile crop with applications spanning textiles, construction, pharmaceuticals, and food products. This study aimed to compile and analyze publicly available Cannabis RNA‐Seq data and develop an integrated database tool to help advance Cannabis research in various topics such as fiber production, cannabinoid biosynthesis, sex determination, and plant development. We identified 515 publicly available RNA‐Seq samples that, after stringent quality control, resulted in a high‐quality dataset of 394 samples. Utilizing the Jamaican Lion genome as reference, we constructed a comprehensive database and developed the Cannabis Expression Atlas (https://cannatlas.venanciogroup.uenf.br/), a web application for visualization of gene expression, annotation, and functional classification. Key findings include the quantification of 27,640 Cannabis genes and their classification into seven expression categories: not‐expressed, low‐expressed, housekeeping, tissue‐specific, group‐enriched, mixed, and expressed‐in‐all tissues. The study revealed substantial variability and coherence in gene expression across different tissues and chemotypes. We found 2,382 tissue‐specific genes, including 177 transcription factors. The Cannabis Expression Atlas constitutes a valuable tool for exploring gene expression patterns and offers insights into Cannabis biology, supporting research in plant breeding, genetic engineering, biochemistry, and functional genomics.
The Impact of Metrics in Mechanical Imaging
Authors: Chabib Ahmed , Witz Jean‐François , Gosselet Pierre , Magnier Vincent 
Published: December 2024

ABSTRACTOptical flow (OF) is an alternative technique to the more classical digital image correlation (DIC) methods, able to measure the motion between two images. It is a pixel‐wise method, in the sense that the displacement is defined at each pixel and its computation does not require overpixel grids. This paper examines the impact on the identified strain field, the main quantity of interest for solid mechanics, of the metrics used to quantify the conservation of the OF and to impose the regularity of the displacement. The Charbonnier and Lorentzian loss functions are inspected in this work. A new regularization approach is used to locally vary the Tikhonov parameter using a mask in order to preserve the discontinuities and encourage the appearance of local phenomena while smoothing the computed fields elsewhere. Finally, an open‐source GPU‐accelerated python code has been implemented.
Effect of concurrent pathogen reduction (amotosalen/UVA) and gamma/x‐ray irradiation on biochemical characteristics of apheresis platelets in additive solution
Authors: Khoshi M. Reza , Skripchenko Andrey , Seifu Robel , Byrne Karen , West‐Mitchell K. , Conry‐Cantilena Cathy , Villa Carlos H. , Simak Jan , Vostal Jaroslav G. 
Published: December 2024

AbstractBackgroundPathogen reduction (PR) may be used as an alternative to gamma or x‐ray irradiation (I) to prevent transfusion associated graft versus host disease (TA‐GVHD) if the pathogen reduction technology has been shown to inactivate residual lymphocytes. However, as I is considered the gold standard for reducing the risk of TA‐GVHD, some centers continue to perform I in addition to PR. This study investigated the effect of concurrent pathogen reduction and irradiation (PR/I) on the biochemical characteristics of apheresis platelets at day 1, 5, and 7 of storage at room temperature.MethodsWe compared in vitro characteristics of apheresis platelets (PLTs), PR PLTs, I PLTs, and PR/I PLTs at storage day 1, 5, and 7. PLTs from six healthy volunteers were suspended in 65% PAS‐3/35% plasma prior to splitting and treatment with PR, I, or PR/I. Parameters measured were: PLT loss, mean PLT volume (MPV), pH, glucose consumption, lactate production, CD62P, annexin V binding, PLT aggregation, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS).ResultsPR/I PLTs did not show significant changes in measured parameters when compared to PR PLTs. However, when compared to control PLTs, PR and PR/I PLTs showed significant declines in PLT content, pH, MMP, aggregation and significant increases in MPV, CD62P, annexin V binding, and ROS production, mostly on day 7 of storage. Irradiation did not cause significant changes in measured parameters in comparison to control PLTs.Conclusions/SummaryWhile PR impacts PLTs' biochemical characteristics and function, irradiation of PR PLTs did not cause additional significant changes.
Long‐term outcome following multimodality treatment in a cat with recurrent laryngeal adenocarcinoma
Authors: Sesanto Christine , Lawrence Jessica , Holkham Jocelyn , Serra Juan Carlos , Bavcar Spela , Parys Magdalena 
Published: December 2024

AbstractA 9.5‐year‐old male neutered domestic short‐haired cat received two courses of postoperative, definitive‐intent conformal radiation therapy (RT) for recurrent laryngeal adenocarcinoma (LACA). Adjuvant RT was prescribed (16 × 3.0 Gy, total 48 Gy) following incomplete resection. Following tumor recurrence and subsequent incomplete resection 31.5 months after the first course, a second course was prescribed (20 × 2.5 Gy, total 50 Gy). Acute and late adverse events were mild. No evidence of local recurrence was documented 42 months following the second course when the cat was euthanized for renal disease. This first report of long‐term control in a cat with LACA supports further evaluation of surgery and definitive‐intent RT.
Computed tomographic findings in dogs with ovarian tumors: A tortuous ovarian artery consistently identifies ovarian origin in complex abdominal masses
Authors: Manfredi Martina , Morabito Simona , Fournier Quentin , Panopoulos Ioannis , Thierry Florence , Schwarz Tobias , Lopez Cristobal , Baldinetti Manuela , Massarenti Chiara , Zani Davide Danilo , Longo Maurizio 
Published: December 2024

AbstractThe aim of this retrospective multicentric case series is to describe the CT findings of ovarian neoplasia in dogs. Twenty dogs with pre‐ and postcontrast CT exams and cytological/histological diagnosis of ovarian neoplasia were included. Five dogs presented with bilateral tumors, for a total of 25 neoplasms: 15 carcinomas (4 bilateral), 4 granulosa cell tumors, 2 poorly differentiated malignant neoplasia (bilateral), 2 luteomas, 1 teratoma, 1 dysgerminoma. In two dogs, the tumor developed from an ovarian remnant. Ovarian tumors showed variable size, lobulated shape, and precontrast heterogenous appearance. Mineral foci and/or fat components were rare, observed in teratoma, granulosa cell tumors (2), and ovarian carcinoma. Tumor type was not found to be associated with any CT features. Larger masses were more likely located in the central abdomen ventral to the ipsilateral kidney, demonstrated signs of tumor rupture, and were associated with abdominal or sternal lymphadenopathy and peritoneal effusion. A tortuous ovarian artery was constantly detectable, associated with an enlarged gonadal vein (12 cases). Related cavitary changes were peritoneal effusion (14 dogs) and sternal lymphadenopathy (7 dogs). Presumed or confirmed metastasis was reported in 9 of 20 cases, with CT evidence of transcoelomic (serosal thickening, peritoneal nodules, omental cake, implant lesions to the liver, spleen, and diaphragm), lymphatic and hematogenous spread (lungs, liver, bone, muscles, and spleen). In conclusion, the present study reports the CT features of different canine ovarian neoplasia. A tortuous ovarian artery may be useful to consistently recognize the ovarian origin of a large abdominal mass.
Synergistic effects of incorporated additives in multifunctional dressings for chronic wound healing: An updated comprehensive review
Authors: Sadeghi‐avalshahr Alireza , Nazarnezhad Simin , Hassanzadeh Halimeh , Kazemi Noughabi Mahboubeh , Namaei‐Ghasemnia Negar , Jalali Mehdi 
Published: December 2024

AbstractDetailed reviewing of the complicated process of wound healing reveals that it resembles an orchestrated symphony via a precise and calculated collaboration of relevant cells at the wound site. The domino‐like function of various cytokines, chemokines, growth factors and small biological molecules such as antibacterial peptides all come together to successfully execute the wound healing process. Therefore, it appears that the use of a wound dressing containing only a single additive with specific properties and capabilities may not be particularly effective in treating the complex conditions that are usual in the environment of chronic wounds. The use of multifunctional dressings incorporating various additives has shown promising results in enhancing wound healing processes. This comprehensive review article explores the synergistic effects of integrated additives in such dressings, aiming to provide an updated understanding of their combined therapeutic potential. By analysing recent advancements and research findings, this review sheds light on the intricate interactions between different additives, their mechanisms of action and their cumulative impact on wound healing outcomes. Moreover, the review discusses the importance of utilising combined therapies in wound care and highlights the potential future directions and implications for research and clinical practice in the field of wound healing management.