bluuebunny/crossref_metadata_2025_split huggingface dataset:
SLICK: A Sandwich-LIke Culturing Kit forin situCryo-ET Sample Preparation
Authors: Li Qiuye , Zhang Li , Xu Qin , Zhang Peng , Zhu Shiwei 
Published: February 2025

AbstractIn situcryo-electron tomography (cryo-ET) has recently been widely used in observing subcellular structures and macromolecules in their native states at high resolution. One of the reasons that it has not been more widely adopted by cell biologists and structural biologists is the difficulties in sample preparation. Here we present the Sandwich-LIke Culturing Kit (SLICK), simplifying the procedure and increasing the throughput for sample preparation forin situcryo-ET (69 words).
Nanopore sequencing-based measurement of paramyxovirus RNA editing reveals virus-specific differences in editing efficiency of mRNA, antigenome and genome
Authors: Masuda Yusuke , Rakib Tofazzal Md. , Akter Lipi , Nakagawa Keisuke , Naitou Kiyotada , Saito Akatsuki , Yamaguchi Ryoji , Matsumoto Yusuke 
Published: February 2025

AbstractParamyxovirus polymerase recognizes an RNA editing signal on the viral genome and transcribes mRNA in which guanine nucleotides are inserted in a template-independent manner. This enables the synthesis of multiple proteins from a single gene, which is important for viral growth. We developed a method to quantify RNA editing efficiency using Oxford Nanopore Technologies’ MinION platform. We performed sequence analysis of reverse transcription (RT)-PCR amplicons with the RNA editing sites in cells infected with Sendai virus (SeV) and canine distemper virus (CDV). By modifying RT primers, we simultaneously assessed RNA editing efficiency in mRNA, antigenome and genome. We observed distinct differences in mRNA editing efficiency between SeV and CDV. Notably, while RNA editing in SeV is confined to mRNA, in CDV it is also observed in antigenome/genome. (Anti)genomes harboring extra nucleotides may deviate from a multiple-of-six sequence, suggesting that RNAs not following the “Rule of Six” are produced in CDV-infected cells.HighlightsA method was established to quantify RNA editing efficiency in RNAs from paramyxovirus-infected cells using the MinION platform.RNA editing efficiency in mRNA differs between Sendai virus and canine distemper virus.Although RNA editing is confined to mRNA during Sendai virus infection, it is also observed in the antigenome and genome during canine distemper virus infection.
Pooled screening identifies combinatorial CAR signaling domains for next-generation CAR-M immunotherapies
Authors: Wang Yuxin , Zuo Shiman , Bao Hanjing , Zhang Zehui , Chen Yijun , Zhang Wenlong , Liu Qi , Lu Yan , Huang Yahong , Zheng Wei , Yang Nanfei , Ye Lupeng , Shen Pingping 
Published: February 2025

AbstractChimeric antigen receptor-engineered macrophages (CAR-Ms) hold great promise for solid tumor immunotherapy. The intracellular domains (ICDs) of CARs determine the phenotypic output of therapeutic macrophages but remain largely unexplored. Here, we constructed a CAR library containing 131 unique signaling domains derived from native immune receptors and identified 17 ICDs that enhance macrophage phagocytosis, inflammatory responses, or tumor infiltrationin vitroandin vivo. We further developed a scalable 3’ barcode technology, CARode, to uniquely label and trace ICD variants within large-scale combinatorial CAR library and applied it to single-cell RNA sequencing and single-cell CAR analysis to assess the synergetic effects of ICD combinations on macrophage activation. Our approach uncovered a novel CD40-LY9-FCRL1 chimeric receptor that modulates the tumor microenvironment and improves solid tumor clearance. In conclusion, our findings demonstrate that pooled screening can accelerate the discovery of complex ICD constructs, providing a powerful platform for engineering macrophage-based immunotherapies.
Structure-based analysis unveils co-origin of LPOR and nitrogenase-like proteins
Authors: Sun Xizhe , Ma Lisong , Zeng Fanli , Hong Yiguo , Dixon Ray , Rao Zihe , Zhao Guogang , Zhao Jin , Zhang Chao , Wu Meng , Ma Chukang , Yu Xiaolan , Yang Ji , Purton Saul , Ermilova Elena , Scrutton Nigel , Silva Pedro , Zhao Jianjun , Cheng Qi 
Published: February 2025

SUMMARYStructures of nitrogenases, dark-operative protochlorophyllide oxidoreductases, and light-dependent protochlorophyllide oxidoreductases (LPOR) have been resolved. However, their evolutionary relatedness remains elusive. Here, we show, through structural alignment, that all subunits of nitrogenase-like proteins originated from a co-ancestral archaic one-subdomain precursor. LPOR evolved from the BchX/BchY subunits of nitrogenase-like chlorophyllideaoxidoreductase (COR), and the intermediary retinol dehydrogenase through possible genetic recombination. We thus establish previously unknown structural links among key enzymes involved in biological nitrogen-fixation (BNF) and photosynthesis, unraveling structure-guided functional evolution from a single-subunit iron protein to multi-subunit nitrogenase-like COR, and to the single-subunit LPOR for phototrophic metabolismviabacteriochlorophyll, retinal, and chlorophyll. This work also demonstrates structural similarities are imperative for inferring distant origins of functionally divergent proteins, particularly those lacking primary amino-acid sequence identity. Moreover, our findings coupled with AI may be exploited to design innovative light-driven CORs and/or light-utilizing nitrogenases with enhanced efficacy of photosynthesis and BNF.Graphical abstractVideo abstracthttps://www.youtube.com/watch?v=b0qBE1JMaH0HighlightsLPOR evolves from BchX and BchY subunits of the nitrogenase-like protein (NGLP) CORAll subunits of NGLPs can be traced back to the ancestral one-subdomain precursorRetinol dehydrogenase acts as a crucial intermediate in the COR to LPOR evolutionStructure-based design of LUN is showcased to revolutionize protein engineering
Artificial intelligence in action: shaping the future of public sector
Authors: Panda Mahanish , Hossain Munshi Maksud , Puri Roma , Ahmad Anees 
Published: February 2025

Purpose
Artificial intelligence (AI) has transformed various sectors, including automotive, finance, media, travel and retail by leveraging new-age technologies. Education, banking, health care, social policy and regulation, within the public sector have witnessed significant AI applications and substantial benefits. The importance of AI in the public sector includes enhanced efficiency, improved decision-making, cost savings, citizen-centric services, etc. Despite these advancements, a mindful discussion on the societal impact of AI in the public sector demands comprehension regarding its subjugation. Therefore, this study aims to analyze the role of AI in transforming the public sector using a bibliometric analysis of recent trends and challenges.

Design/methodology/approach
This study has used bibliometric analysis to trace the intellectual patterns of previous research. It comprises 231 articles from 2000 to 2024 from Scopus through the Scientific Procedures and Rationales for Systematic Literature Reviews protocol. This protocol has adopted a three-step process for identifying articles, i.e. assembling, arranging and assessing.

Findings
The publication trend shows an upward trajectory since 2017, whereas network visualization protrudes with the recent trends and thematic expressions, namely, Global AI ethics and policy challenges in public sectors, AI adoption and governance in public sector, challenges and opportunities of implementing AI in public administration and AI’s role in economic and public transformation.

Research limitations/implications
The findings suggest AI adoption in the public sector enhances transparency and efficiency but demands ethical guidelines, legal frameworks and stakeholder governance to address challenges such as data privacy, algorithmic bias and public trust. Policies should promote responsible AI use, balancing innovation with accountability to improve public service delivery and uphold democratic values.

Originality/value
This paper enhances the limited literature on the integration of AI in the public sector, focusing on emerging themes and trending topics with future research directions to furnish a holistic perspective. It aims to guide researchers and policymakers in exploring areas for further investigation in this domain.
Intercultural sensitivity and sociocultural adaptation among cabin attendants: direct, indirect and interaction effects on organizationally valued job outcomes
Authors: Lee Gyehee , Karatepe Osman M. , Kim Jieun , Olorunsola Victor Oluwafemi , Kim Taegoo Terry 
Published: February 2025

Purpose
This paper aimed to propose a research model that investigated job satisfaction (JSAT) and affective organizational commitment (AOC) as the serial mediators linking intercultural sensitivity (IS) to service-oriented organizational citizenship behavior (SOCB). It also explored sociocultural adaptation (SA) as a moderator of the influence of IS on JSAT.

Design/methodology/approach
Data gathered from Korean cabin attendants employed at Middle Eastern airlines were utilized to gauge the aforementioned relationships via structural equation modeling.

Findings
Cabin attendants high on IS were satisfied with their jobs and therefore exhibited affective commitment to their airline company. Such employees in turn displayed heightened SOCB. SA strengthened the positive influence of IS on JSAT.

Practical implications
The presence of interactive training, scenario-based simulations and cross-cultural communication exercises would enable cabin attendants to manage passengers with different cultural background successfully.

Originality/value
This empirical piece contributes to the pertinent literature by investigating the mechanisms underlying the link between IS and organizationally valued behavioral outcomes such as SOCB. It also enhances the understanding about SA which increases the positive impact of IS on JSAT.
Multi-dimensional organizational motivations: the catalyst for interorganizational knowledge sharing in megaprojects
Authors: He Hui , He Qinghua , Chen Yajiao , Wang Ge , Chen Xiaoyan 
Published: February 2025

PurposeInterorganizational knowledge sharing (IKS) is an important strategy for organizations to obtain exterior knowledge resources to enhance innovation and value co-creation in megaprojects. However, little research has deeply investigated multiple attributes of organizational motivations and the combinatorial effects of these motivations on facilitating IKS. Based on resource dependency theory, this study examined the net and combinatorial effects of four types of motivations (i.e. image motivations, reactive motivations, project-based co-creative economic motivations and cross-project co-creative economic motivations).Design/methodology/approachQuestionnaire surveys were administered to 244 practitioners with over 10 years of experience in construction megaprojects. The collected data were analyzed using a partial least square-structural equation model (PLS-SEM) and fuzzy set qualitative comparative analysis (fsQCA).FindingsThe findings of PLS-SEM support that image and project-based co-creative economic motivations positively drive IKS. The results of fsQCA reveal three types of efficacious configurations, including comprehensive benefits-integrated driven, short-term benefits-dominated driven and long-term benefits-dominated driven configurations.Originality/valueThis study provides insight into broadening the understanding of multidimensional organizational motivations for IKS and fostering the configurational perspective to their effects with the idea that one size does not fit all. Managers should develop effective interventions considering the combination of various motivations and use multi-layered incentive structures that reward both immediate project-specific outcomes and long-term relationship-building efforts.
The impact of exogenous shocks on strategy, business models and product development in the Portuguese footwear industry
Authors: Oliveira Jorge , Pereira Cláudia , Oliveira Ana 
Published: February 2025

Purpose
This study aims to investigate the footwear industry’s resilience to external changes in an attempt to provide insights into strategies that can define effective organizational responses to environmental stimuli. In this sense, it is important to understand how companies have adapted to the exogenous shocks. Thus, this paper addresses the impact of the exogenous shocks like COVID-19 pandemic, the Russia–Ukraine war and energy crisis in the European Union and focuses on the potential resilience strategies implemented by the footwear industry.

Design/methodology/approach
Through a qualitative approach, 3 interviews were carried out on the structural basis of the footwear industry, namely at an associative and technological level, and 11 in footwear companies.

Findings
This study shows that the exogenous shocks forced changes in the nature of business, namely: (1) manufacturers entered the B2C segment, shortening the supply chain; (2) it provided changes at the strategic level (greater focus on the development of operational and logistics strategies) and digitization (digital transformation to support the execution of essential activities); (3) development of new products (emergence of a market opportunity that combines the concepts of sporty and classic shoes).

Research limitations/implications
This study is limited to the footwear industry, does not address how cultural, social or behavioral differences in specific markets may impact adaptation to exogenous shocks, and focuses on recent exogenous shocks, without analyzing similar past events for historical comparisons. It also does not explore the direct financial impact of the suggested strategies.

Practical implications
This study focuses on strategic business adaptation in crisis scenarios, showing the importance of flexibility and resilience, suggesting specific practices to improve supply chain resilience. This can help companies reduce vulnerabilities in the face of global disruptions.

Social implications
This study provides an important contribution to contingency theory, by demonstrating that companies need to adjust their structures and strategies in response to exogenous shocks.

Originality/value
This study focuses on the concept of resilience to find out what strategies should be adopted to respond to external changes.
Unveiling excellence in Indian healthcare: a patient-centric PRISMA analysis of hospital service quality, patient satisfaction and loyalty
Authors: Divya Divya , Savita Savita , Kaur Sandeepa 
Published: February 2025

Purpose
This paper aims to provide a conceptual framework containing SERVQUAL original dimensions and add two additional dimensions: patient satisfaction and loyalty in the hospital SQ model that demonstrates the relationship between hospital service quality, patient satisfaction and loyalty from patients’ perspective.

Design/methodology/approach
This research conducted a thorough literature review using specific keywords and electronic databases, adhering to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Through analysis, the key dimensions of service quality in Indian hospitals were identified, with the addition of patient satisfaction and loyalty as variables. Of 1,000 initially downloaded papers, 497 were included.

Findings
While many researchers rely on the SERVQUAL model, some introduce new or modified dimensions, often renaming existing ones. This study identifies the RATER factors as the main dimensions patients use to evaluate hospital services. This study finds a positive relationship between service quality, patient satisfaction and loyalty.

Practical implications
An understanding of how health-care service quality dimensions, directly and indirectly, affect patient satisfaction and loyalty is important for hospital marketing managers. This study helps them take action to improve patient satisfaction, which encourages patients to be loyal.

Originality/value
This research provides a comprehensive framework for measuring health-care service quality, combining SERVQUAL dimensions and new variables. This study offers useful insights for academics and health-care professionals, promoting more accurate measurement and enhancement of service quality. The use of PRISMA in this context is also innovative, as it is less common in administrative health-care research.
Unveiling the mediating mechanism of burnout in relationship between work–family conflict and job outcomes among millennials workers at work
Authors: Zainal Badri Siti Khadijah , Ngo Michelle She Min 
Published: February 2025

PurposeThis paper investigates the linkage between work–family conflict, turnover intention (TOI) and job satisfaction (JS) in the context of millennial workers. It also examines the role of burnout as a mediator in this relationship, acknowledging the unique struggles of this generation.Design/methodology/approachAbout 352 millennials were recruited for this study. Data were collected using an online survey, and analysis was performed using path analysis and structural equation modeling.FindingsThis study found a significant association between work-to-family conflict with TOI and JS but not family-to-work conflict. Burnout is found to be a partial mediator for the relationship between work-to-family conflict and the outcomes. In comparison, it is a full mediator for family-to-work conflict.Practical implicationsFindings from this study reveal that work–family conflict influences millennials’ job satisfaction and turnover intention through their burnout experience. Hence, organizations should focus on strategies aiming to mitigate work-family conflict and burnout.Originality/valueThis study unveils the mechanism of burnout in explaining work–family conflict and the JS and TOI of millennial workers. It outlines suggestions and ways to manage millennials at work through a responsive environment toward both their work and life aspects.
Does time orientation improve Islamic cultural finance
Authors: Al-Hersh Nafith Fayez 
Published: February 2025

Purpose
Time orientation as a proxy of culture can play an important role in promoting the industry and underpin the new field of “Islamic cultural finance.” This paper aims to investigate the effect of time orientation on Islamic finance.

Design/methodology/approach
The dynamic generalized method of moments was used to study the influence of time orientation in 45 countries from 2012–2020 in the main analysis. Fixed-Effect Model was applied for the regional analysis.

Findings
Results indicate the existence of a significant effect of “time orientation” on Islamic finance. In regional comparison analysis, time orientation is not significant in some regions, such as non-Asian and Arab countries. The market is very limited in non-Asian countries, while similarities in scope between Islamic and conventional finance reduce the long-term orientation in Arab countries.

Practical implications
Improving the time orientation of weak countries enhances the industry’s status in those countries and pushes it to compete with the leading countries in the industry. Culture is a comprehensive framework that includes all factors located within it. While governance is imposed through regulation and procedures and raises the cost of business, culture is integral and built-in, and thus it is less expensive, and its presence is stronger, facilitating the applications of Islamic finance.

Originality/value
This paper proposed time orientation as a proxy for culture and extended the new domain of Islamic cultural finance using quantum (econometric) methods instead of surveys and questionnaires.
Intention toward sustainable entrepreneurship in Vietnam
Authors: Bui Thi Thu Trang , Delladio Silvia , Serpico Davide , Trento Sandro 
Published: February 2025

Purpose
This study aims to explore the factors influencing sustainable entrepreneurial intentions in Vietnam.

Design/methodology/approach
A survey of 330 university students in Vietnam was conducted, and the data were analyzed using multiple regressions. Applying the theory of planned behavior and the entrepreneurial event model as theoretical lens, the findings suggest that attitude toward sustainability and perceived entrepreneurial feasibility influence positively sustainability-oriented entrepreneurial intentions.

Findings
Contrary to what expected, perceived entrepreneurial desirability has no impact on sustainability-oriented entrepreneurial intentions. In addition, attitude toward sustainability is positively influenced by altruism and intrinsic rewards, whereas perceived entrepreneurial feasibility is positively related to self-efficacy and negatively related to job security. Finally, perceived entrepreneurial desirability is positively influenced by intrinsic rewards.

Originality/value
The research applies the entrepreneurial intention model to sustainable entrepreneurship in the Vietnamese context, highlighting the need to consider variations in entrepreneurial opportunities in intention models and the potential conflict between different work values.
Gender impact on business growth in Europe and the Middle East and North Africa
Authors: Gilani Sayed Abdul Majid , Mohamed Hashim Mohamed Ashmel , Tlemsani Issam , Tantry Ansarullah , Whitehead James 
Published: February 2025

Purpose
The purpose of this study is to analyse how gender diversity within corporate boardrooms influences business growth, specifically comparing the contexts of Europe and the Middle East and North Africa (MENA) region. It aims to explore the dynamics of gender representation in leadership roles, evaluate its impact on organizational performance and assess how cultural and socio-economic factors affect gender diversity in these distinct regions. The study seeks to provide insights that can inform policies promoting gender equality in leadership, contributing to improved decision-making and potentially enhancing business outcomes.

Design/methodology/approach
Using a quantitative and comparative approach, this study includes responses from 430 business leaders, with 210 from MENA and 220 from Europe. Data were collected through structured surveys and analysed using SPSS to determine the correlation between gender diversity in boardrooms and business growth. Data were collected through structured surveys and analysed using analysis of variance in SPSS to determine the potential gender diversity in boardrooms and business growth.

Findings
The analysis reveals a strong male dominance in decision-making roles in both European and MENA businesses, with men holding most power and influence. European boardrooms show slightly more gender diversity than MENA, though this does not consistently correlate with higher business growth. Interestingly, MENA reports stronger growth despite less diversity. Women leaders in both regions are effective and dedicated but remain underrepresented.

Research limitations/implications
The findings call for region-specific policies to enhance gender diversity in leadership, with Gender Equality Action Plans tailored to Europe and MENA. Businesses should adopt mentorship and inclusive policies to support growth through diverse leadership. Promoting diversity can drive workplace equality, supported by education. Further research on long-term impacts and stakeholder engagement is recommended to foster change.

Originality/value
This research enhances literature by examining women’s representation in gender-balanced boardrooms and addressing the East-West divide. It fills gaps with practical, policy and theoretical recommendations, offering valuable insights for businesses and policymakers promoting gender equality in leadership.