bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low serum miR‐223 expression predicts poor outcome in patients with acute myeloid leukemia
Authors: Yu Guopan , Yin Zhao , He Han , Zheng Zhongxin , Chai Yanyan , Xuan Li , Lin Ren , Wang Qiang , Li Jie , Xu Dan 
Published: November 2019

AbstractBackgroundIdentification of biomarkers for acute myeloid leukemia (AML) is important for treating this malignancy. Recent studies have reported that microRNAs (miRNAs) are stably detectable in the blood/plasma and can be used as biomarkers for various types of cancer including AML. The aim of this study was to analyze miR‐223 level in serum as a potential indicator for AML diagnosis and prognosis prediction.MethodsQuantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) was used to detect the levels of miR‐223 in the serum samples from 131 patients and 70 healthy individuals.ResultsThe results revealed that serum miR‐223 was underexpressed in AML patients, particularly those in intermediate and unfavorable cytogenetic risk groups. Further analysis revealed that serum miR‐223 could yield a receiver operating characteristic (ROC) area under the curve (AUC) of 0.849 with 83.2% sensitivity and 81.4% specificity. Moreover, a significant increase in serum miR‐223 level was observed in AML subjects after their treatment. Reduced serum miR‐223 level was highly associated with aggressive clinical variables and shorter survival of patients. Furthermore, miR‐223 expression was identified to be an independent prognostic predictor of worse overall survival.ConclusionIn conclusion, miR‐223 may be a reliable diagnostic and prognostic biomarker for AML.
miR‐4286 promotes prostate cancer progression by targeting the expression of SALL1
Authors: Li Zhanqi , Zhao Shaoxiong , Wang Hui , Zhang Binbin , Zhang Peibo 
Published: November 2019

AbstractBackgroundProstate cancer (PCa) is a serious health threat for humans worldwide. Recent studies have revealed that microRNAs are associated with the progression of human cancers, including PCa. However, no study has been performed aiming to investigate the role of microNA‐4286 (miR‐4286) on PCa.MethodsA quantitative reverse transcriptase‐polymerase chain reaction was conducted to analyze the expression level of miR‐4286 in PCa cells. The connection of miR‐4286 and spalt like transcription factor 1 (SALL1) was analyzed with a bioinformatic analysis tool, a dual‐luciferase activity reporter assay and western blotting. The effects of miR‐4286 and SALL1 on PCa cell behaviors were examined in vitro.ResultsWe showed miR‐4286 expression was significantly increased in PCa cells compared to a normal cell line. Knockdown of miR‐4286 could inhibit PCa cell proliferation but promote cell apoptosis by targeting SALL1.ConclusionsThe results of the present study suggest that miR‐4286 overexpression represents a tumor promoter role in PCa.
Interplay between the RNA binding‐protein Musashi and developmental signaling pathways
Authors: das Chagas Pablo Ferreira , Baroni Mirella , Brassesco María Sol , Tone Luiz Gonzaga 
Published: November 2019

AbstractMusashi comprises an evolutionarily conserved family of RNA‐binding proteins (RBP) that regulate cell fate decisions during embryonic development and play key roles in the maintenance of self‐renewal and differentiation of stem cells and adult tissues. More recently, several studies have shown that any dysregulation of MSI1 and MSI2 can lead to cellular dysfunctions promoting tissue instability and tumorigenesis. Moreover, several reports have characterized many molecular interactions between members of the Musashi family with ligands and receptors of the signaling pathways responsible for controlling normal embryonic development: Notch, Transforming Growth Factor Beta (TGF‐β), Wingless (Wnt) and Hedgehog Signaling (Hh); all of which, when altered, are strongly associated with cancer onset and progression, especially in pediatric tumors. In this context, the present review aims to compile possible cross‐talks between Musashi proteins and members of the above cited molecular pathways for which dysregulation plays important roles during carcinogenesis and may be modulated by these RBP.
Neural substrates of habit
Authors: Malvaez Melissa 
Published: November 2019

AbstractActive reward pursuit is supported by the balance between the cognitive and habitual control of behavior. The cognitive, goal‐directed strategy relies on the prospective evaluation of anticipated consequences, which allows behavior to readily adapt when circumstances change. Repetition of successful actions promotes less cognitively taxing habits, in which behavior is automatically executed without prospective consideration. Disruption in either of these behavioral regulatory systems contributes to the symptoms that underlie many psychiatric disorders. Here, I review recently identified neural substrates, at multiple neural levels, that contribute to habits and outline gaps in knowledge that must be addressed to fully understand the neural mechanisms of behavioral control.
Sulfasalazine Resolves Joint Stiffness in a Rabbit Model of Arthrofibrosis
Authors: Atluri Keerthi , Brouillette Marc J. , Seol Dongrim , Khorsand Behnoush , Sander Edward , Salem Aliasger K. , Fredericks Douglas , Petersen Emily , Smith Sonja , Fowler Timothy P. , Martin James A. 
Published: November 2019

ABSTRACTJoint stiffness due to fibrosis/capsule contracture is a seriously disabling complication of articular injury that surgical interventions often fail to completely resolve. Fibrosis/contracture is associated with the abnormal persistence of myofibroblasts, which over‐produce and contract collagen matrices. We hypothesized that intra‐articular therapy with drugs targeting myofibroblast survival (sulfasalazine), or collagen production (β‐aminopropionitrile and cis‐hydroxyproline), would reduce joint stiffness in a rabbit model of fibrosis/contracture. Drugs were encapsulated in poly[lactic‐co‐glycolic] acid pellets and implanted in joints after fibrosis/contracture induction. Capsule α‐smooth muscle actin (α‐SMA) expression and intimal thickness were evaluated by immunohistochemistry and histomorphometry, respectively. Joint stiffness was quantified by flexion‐extension testing. Drawer tests were employed to determine if the drugs induced cruciate ligament laxity. Joint capsule fibroblasts were tested in vitro for contractile activity and α‐SMA expression. Stiffness in immobilized joints treated with blank pellets (control) was significantly higher than in non‐immobilized, untreated joints (normal) (p = 0.0008), and higher than in immobilized joints treated with sulfasalazine (p = 0.0065). None of the drugs caused significant cruciate ligament laxity. Intimal thickness was significantly lower than control in the normal and sulfasalazine‐treated groups (p = 0.010 and 0.025, respectively). Contractile activity in the cells from controls was significantly increased versus normal (p = 0.001). Sulfasalazine and β‐aminopropionitrile significantly inhibited this effect (p = 0.005 and 0.0006, respectively). α‐SMA expression was significantly higher in control versus normal (p = 0.0021) and versus sulfasalazine (p = 0.0007). These findings support the conclusion that sulfasalazine reduced stiffness by clearing myofibroblasts from fibrotic joints. Statement of clinical significance: The results provide proof‐of‐concept that established joint stiffness can be resolved non‐surgically. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:629–638, 2020
Association between periodontitis and metabolic syndrome: A case‐control study
Authors: Campos Julya Ribeiro , Costa Fernando Oliveira , Cota Luís Otávio Miranda 
Published: November 2019

AbstractBackgroundOver the past few years, the association between metabolic disorders and periodontitis has been widely studied and recognized. However, the relationship between metabolic syndrome (MetS) and periodontitis remains controversial. The aim of the present case‐control (1:3) study is to evaluate the potential association between periodontitis and MetS, as well as the influence of risk variables on this association.MethodsSamples comprised 122 individuals with MetS (cases) and 366 controls. Plaque index, probing depth, clinical attachment level, and bleeding on probing were evaluated through a full‐mouth periodontal examination. The association between risk variables, MetS, and periodontitis was tested through univariate analysis and multivariate logistic regression.ResultsA high prevalence of periodontitis was observed among cases (54.6%) when compared with controls (45.4%). Significant variables associated with the occurrence of periodontitis in the final logistic model were: MetS (odds ratio [OR] = 2.02; P = 0.003), number of teeth ≤14 (OR = 1.78; P = 0.034), and smoking (OR = 2.19; P = 0.001).ConclusionsAn important risk association between MetS and periodontitis was reported, being that individuals with MetS presented worse periodontal status and higher prevalence, severity, and extension of periodontitis.
Recent advances and trends in miniaturized sample preparation techniques
Authors: Soares da Silva Burato Juliana , Vargas Medina Deyber Arley , de Toffoli Ana Lúcia , Vasconcelos Soares Maciel Edvaldo , Mauro Lanças Fernando 
Published: November 2019

AbstractAdvances in the area of sample preparation are significant and have been growing significantly in recent years. This initial step of the analysis is essential and must be carried out properly, consisting of a complicated procedure with multiple stages. Consequently, it corresponds to a potential source of errors and will determine, at the end of the process, either a satisfactory result or a fail. One of the advances in this field includes the miniaturization of extraction techniques based on the conventional sample preparation procedures such as liquid‐liquid extraction and solid‐phase extraction. These modern techniques have gained prominence in the face of traditional methods since they minimize the consumption of organic solvents and the sample volume. As another feature, it is possible to reuse the sorbents, and its coupling to chromatographic systems might be automated. The review will emphasize the main techniques based on liquid‐phase microextraction, as well as those based upon the use of sorbents. The first group includes currently popular techniques such as single drop microextraction, hollow fiber liquid‐phase microextraction, and dispersive liquid‐liquid microextraction. In the second group, solid‐phase microextraction techniques such as in‐tube solid‐phase microextraction, stir bar sorptive extraction, dispersive solid‐phase extraction, dispersive micro solid‐phase microextraction, and microextraction by packed sorbent are highlighted. These approaches, in common, aim the determination of analytes at low concentrations in complex matrices. This article describes some characteristics, recent advances, and trends on miniaturized sample preparation techniques, as well as their current applications in food, environmental, and bioanalysis fields.
Joint forcing by heat waves and mowing poses a threat to grassland ecosystems: Evidence from a manipulative experiment
Authors: Qu Luping , Dong Gang , De Boeck Hans J. , Tian Li , Chen Jiquan , Tang Huajun , Xin Xiaoping , Chen Jinqiang , Hu Yalin , Shao Changliang 
Published: November 2019

AbstractThe frequency and intensity of heat waves (HWs) have increased in recent years, but it remains unclear how grassland ecosystems respond to such extreme weather. A 3‐year manipulative field experiment was conducted to simulate HWs under different mowing intensities in a Stipa krylovii steppe on the Mongolian Plateau to examine their effects on plant morphology, phenology, and community. At the species level, the morphology and phenology of the three main herb species (S. krylovii, Melilotoides ruthenica, and Potentilla tanacetifolia) showed species‐specific responses to the HW and mowing treatments. The major dominant species S. krylovii shed ~50% of the tiller outer layer to protect the internal tiller from HW stress thereby directly decreasing the heat load and water loss from green plant tissue and indirectly increasing the litter biomass. HWs also caused increases of community index (richness, diversity, and evenness) but also associated with a 30% decrease in the importance value of S. krylovii, whereas mowing enhanced this value by 27%. When HWs were combined with mowing, the joint forcing of mechanical damage and low carbon accumulation aggravated negative effects of stress on plant health and growth, which further decreased community index. We constructed a framework to fully describe the effects of HWs and mowing and their interrelationship on different ecological levels and explain how short‐term effects, such as extreme climate, produce long‐term effects on ecosystems. In conclusion, we found that synergisms between climate extremes (HWs) and human activities (mowing) can reduce ecosystem stability posing a threat to the grasslands.
Dry‐braking properties of resin based friction materials by different fibres
Authors: Cui Gongjun , Liu Huiqian , Shi Ruibo , Li Sai , Han Junrui 
Published: November 2019

AbstractIn this study, the cashew nut shell oil modified phenolic resin based friction materials reinforced by aramid pulp/glass/carbon fibres (constant content 17 vol.%) are prepared by hot pressed sintering method, and the effect of different fibres on tribological properties of resin matrix materials is studied. The tribological properties of friction materials are carried out by a ring‐on‐block tribo‐tester sliding against AISI 51200 steel ring under different braking loads and speeds. The friction coefficient and wear rate increase with the increase of load and speed. The synergistic effect of aramid pulp fibre and carbon fibre improves the tribological properties of friction materials. However, the friction coefficient and wear rate increase as the glass fibre increases. The specimen with 9%/1%/7% of aramid pulp/glass/carbon fibres shows more reasonable friction coefficient (0.31–0.50) and lower wear rate (4.5–7.9 × 10−6 mm3 N−1 m−1) than that of commercial friction material.
The Development and Outcome of Acute‐on‐Chronic Liver Failure After Surgical Interventions
Authors: Klein Leah Maria , Chang Johannes , Gu Wenyi , Manekeller Steffen , Jansen Christian , Lingohr Philipp , Praktiknjo Michael , Kalf Jörg C. , Schulz Martin , Spengler Ulrich , Strassburg Christian , Cárdenas Andrés , Arroyo Vicente , Trebicka Jonel 
Published: November 2019

Acute‐on‐chronic liver failure (ACLF) is a syndrome with high short‐term mortality. Precipitating events, including hemorrhage and infections, contribute to ACLF development, but the role of surgery remains unknown. We investigated the development of ACLF in patients with cirrhosis undergoing surgery. In total, 369 patients with cirrhosis were included in the study. The clinical and laboratory data were collected prior to and on days 1‐2, 3‐8, and 9‐28, and at 3 and 12 months after surgery. Surgery type was classified as limited or extensive, as well as liver and nonliver surgery. A total of 39 patients had baseline ACLF. Surgery was performed during acute decompensation in 35% of the rest of the 330 patients, and 81 (24.5%) developed ACLF within 28 days after surgery. Surrogate markers of systemic inflammation were similar in patients who developed ACLF or not. Age, sex, serum sodium, baseline bacterial infection, and abdominal nonliver surgery were independent predictors for the development of ACLF after surgery. Patients who developed ACLF within 28 days after surgery had a higher mortality at 3, 6, and 12 months. Survival did not differ between patients with ACLF at surgery and those developing ACLF after surgery. Development of ACLF within 28 days after surgery and elevated alkaline phosphatase and international normalized ratio were independent predictors of 90‐day mortality. Independent predictors of 1‐year all‐cause mortality were alkaline phosphatase, Model for End‐Stage Liver Disease score, and preoperative hepatic encephalopathy, whereas nonliver surgery was associated with improved survival. ACLF frequently develops in patients with cirrhosis undergoing surgery, especially in those with active bacterial infection, lower serum sodium, and kidney or coagulation dysfunction. Prognoses of ACLF both at and after surgery are similarly poor. Patients with cirrhosis should be carefully managed perioperatively.
Polymerization of Bulky of Oxirane Monomers Leading to Polyethers Exhibiting Intramolecular Charge Transfer Interactions
Authors: Merlani Maia , Song Zhiyi , Wang Yuting , Yuan Yuehui , Luo Jiyue , Barbakadze Vakhtang , Chankvetadze Bezhan , Nakano Tamaki 
Published: November 2019

AbstractThree novel oxirane monomers, 2‐methoxycarbonyl‐3‐(3,4‐dibenzyloxyphenyl)oxirane (MDBPO), 2‐benzyloxycarbonyl‐3‐(3,4‐dibenzyloxyphenyl)oxirane (BDBPO), and 2‐t‐butyloxycarbonyl‐3‐(3,4‐dibenzyloxyphenyl)oxirane (TBDBPO) are prepared and polymerized using BF3‐OEt2 in CH2Cl2 to yield polyethers. The monomers smoothly lead to polymers under the cationic conditions in spite of the monomers' rather bulky structures. The obtained polymers exhibit characteristic UV spectra with intramolecular charge transfer (ICT) bands through hetero π‐stacked structure formed between the side‐chain carbonyl group and the phenyl group directly connects to the main chain. The relative intensity of the ICT bands is the strongest for poly(TBDBPO) having bulky t‐butyl ester group. This spectral feature is considered to have connection with rigidity of polymer chain. Poly(TBDBPO) having the bulkiest side‐chain group is considered to possess the most rigid chain conformation that can account for its strongest ICT interactions that may occur between the carbonyl group and the phenyl group directly attached to the main chain. The proposed rigid chain conformation of poly(TBDBPO) is supported by the fact that the polymer does not show clear Tg while the other two polymers do.
The picture of luxury: A comprehensive examination of college student consumers' relationship with luxury brands
Authors: Eastman Jacqueline K. , Shin Hyunju , Ruhland Kristen 
Published: November 2019

AbstractThis study examines college student consumers' relationship with luxury brands through two studies. Study 1 analyzes collages to determine what represents luxury to them, how consumers perceive their relationships with luxury, and who they are as luxury consumers. Study 2 analyzes qualitative interviews to validate the findings of Study 1 and to add further insights. Results of Study 1 suggest that college student consumers represent a vibrant segment in the luxury market. These consumers perceive a wide variety of products and brands as meeting their luxury needs. They are currently interested in luxury and their potential will only increase as their incomes do. Results of Study 2 confirms their views of luxury and emphasizes the critical roles social media, peers, and family play in influencing college student consumers' luxury consumption and provide insights for how to build an emotional bond with them. Luxury marketers can build brand relationships with college student consumers by offering them entry‐level products as they are current luxury consumers and see their consumption expanding in the future. Given that college student consumers are both vulnerable and savvy in recognizing when they are being manipulated, caution needs to be taken in approaching this segment in relationship‐building efforts.
Nano‐Crystalline Sandwich Formed in Polylactic Acid Fibers
Authors: Zhao Renhai , He Hongwei , Cai Ming , Miao Dagang , Yuan Ding , Ming Jingfa , Wang Na , Ning Xin 
Published: November 2019

AbstractFibers have traditionally been made through melt or solution processes from macromolecules. Most of these fibers have crystalline domains where the segregation of different crystalline features is extremely difficult due to the statistical nature of the formation and growth of these domains. A fibrous nano‐crystalline sandwich is reported where distinctly different crystalline regions are formed in layers along the continuous fiber direction during the spinning process and locked in place. This approach employs side‐by‐side bicomponent nanofiber electrospinning where the components are the enantiomeric pair of poly(l‐lactic acid) and poly(d‐lactic acid). The formation of the poly(lactic acid) (PLA) stereo‐complexes at the junction interphase of the two components is demonstrated through diffusion, which subsequently crystallize into continuous sandwich domains. The stereo‐complex crystalline core in the fiber possesses a melting point 50 °C higher than, and properties substantially different from, the regular PLAs at the fringe areas of the fiber. This nano‐crystalline sandwich fiber structure can be scaled to the micrometers in a commercial bicomponent process.
Blood Biomarkers With Parkinson's Disease Clusters and Prognosis: The Oxford Discovery Cohort
Authors: Lawton Michael , Baig Fahd , Toulson Greg , Morovat Alireza , Evetts Samuel G. , Ben‐Shlomo Yoav , Hu Michele T. 
Published: November 2019

AbstractBackgroundPredicting prognosis in Parkinson's disease (PD) has important implications for individual prognostication and clinical trials design and targeting novel treatments. Blood biomarkers could help in this endeavor.MethodsWe identified 4 blood biomarkers that might predict prognosis: apolipoprotein A1, C‐reactive protein, uric acid and vitamin D. These biomarkers were measured in baseline serum from 624 Parkinson's disease subjects (median disease duration, 1.0 years; interquartile range, 0.5–2.0) from the Oxford Discovery prospective cohort. We compared these biomarkers against PD subtypes derived from clinical features in the baseline cohort using data‐driven approaches. We used multilevel models with MDS‐UPDRS parts I, II, and III and Montreal Cognitive Assessment as outcomes to test whether the biomarkers predicted subsequent progression in motor and nonmotor domains. We compared the biomarkers against age of PD onset and age at diagnosis. The q value, a false‐discovery rate alternative toPvalues, was calculated as an adjustment for multiple comparisons.ResultsApolipoprotein A1 and C‐reactive protein levels differed across our PD subtypes, with severe motor disease phenotype, poor psychological well‐being, and poor sleep subtype having reduced apolipoprotein A1 and higher C‐reactive protein levels. Reduced apolipoprotein A1, higher C‐reactive protein, and reduced vitamin D were associated with worse baseline activities of daily living (MDS‐UPDRS II).ConclusionBaseline clinical subtyping identified a pro‐inflammatory biomarker profile significantly associated with a severe motor/nonmotor disease phenotype, lending biological validity to subtyping approaches. No blood biomarker predicted motor or nonmotor prognosis. © 2019 The Authors.Movement Disorderspublished by Wiley Periodicals, Inc. on behalf of International Parkinson and Movement Disorder Society.