bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urine Proteome of COVID-19 Patients
Authors: Li Yanchang , Wang Yihao , Liu Huiying , Sun Wei , Ding Baoqing , Zhao Yinghua , Chen Peiru , Zhu Li , Li Zhaodi , Li Naikang , Chang Lei , Wang Hengliang , Bai Changqing , Xu Ping 
Published: May 2020

SUMMARYThe atypical pneumonia (COVID-19) caused by SARS-CoV-2 is an ongoing pandemic and a serious threat to global public health. The COVID-19 patients with severe symptoms account for a majority of mortality of this disease. However, early detection and effective prediction of patients with mild to severe symptoms remains challenging. In this study, we performed proteomic profiling of urine samples from 32 healthy control individuals and 6 COVID-19 positive patients (3 mild and 3 severe). We found that urine proteome samples from the mild and severe COVID-19 patients with comorbidities can be clearly differentiated from healthy proteome samples based on the clustering analysis. Multiple pathways have been compromised after the COVID-19 infection, including the dysregulation of immune response, complement activation, platelet degranulation, lipoprotein metabolic process and response to hypoxia. We further validated our finding by directly comparing the same patients’ urine proteome after recovery. This study demonstrates the COVID-19 pathophysiology related molecular alterations could be detected in the urine and the potential application of urinary proteome in auxiliary diagnosis, severity determination and therapy development of COVID-19.
A comprehensive portrait of antimicrobial resistance in the zoonotic pathogenStreptococcus suis
Authors: Hadjirin Nazreen F. , Miller Eric L. , Murray Gemma G. R. , Yen Phung L. K. , Phuc Ho D. , Wileman Thomas M. , Hernandez-Garcia Juan , Williamson Susanna M. , Parkhill Julian , Maskell Duncan J. , Zhou Rui , Fittipaldi Nahuel , Gottschalk Marcelo , Tucker A.W. (Dan) , Hoa Ngo Thi , Welch John J. , Weinert Lucy A. 
Published: May 2020

AbstractAntimicrobial resistance (AMR) is among the gravest threats to human health and food security worldwide. Pigs receive more antimicrobials than most other livestock, and are a known source of zoonotic disease. We studied AMR inStreptococcus suis, a commensal found in most pigs, but which can also cause serious disease in both pigs and humans. We obtained replicated measures of Minimum Inhibitory Concentration (MIC) for 16 antibiotics, across a panel of 678 isolates, from the major pig-producing regions of the world. For several drugs, there was no natural separation into “resistant” and “susceptible”, highlighting the need to treat MIC as a quantitative trait. We found differences in MICs between countries, consistent with their patterns of antimicrobial usage. AMR levels were high even for drugs not used to treatS. suis, with many multi-drug resistant isolates. And similar levels of resistance were found in pigs and humans from zoonotic regions. We next used whole genome sequences for each isolate to identify 43 candidate resistance determinants, 22 of which were novel inS. suis. The presence of these determinants explained most of the variation in MIC. But there were also complications, including epistatic interactions, where known resistance alleles had no effect in some genetic backgrounds. Beta-lactam resistance involved many variants of small effect, appearing in a characteristic order. Our results confirm the potential for genomic data to aid in the fight against AMR, but also demonstrate that it cannot be tackled one species or one drug at a time.
Repurposing low–molecular-weight drugs against the main protease of severe acute respiratory syndrome coronavirus 2
Authors: Gao Jia , Zhang Liang , Liu Xiaodan , Li Fudong , Ma Rongsheng , Zhu Zhongliang , Zhang Jiahai , Wu Jihui , Shi Yunyu , Pan Yueyin , Ge Yushu , Ruan Ke 
Published: May 2020

AbstractThe coronavirus disease (COVID-19) pandemic caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected the global healthcare system. Drug repurposing is a feasible method for emergency treatment. As low–molecular-weight drugs have high potential to completely match interactions with essential SARS-CoV-2 targets, we propose a strategy to identify such drugs using the fragment-based approach. Herein, using ligand- and protein-observed fragment screening approaches, we identified niacin and hit1binding to the catalytic pocket of the main protease of the SARS-CoV-2 (Mpro), thereby modestly inhibiting the enzymatic activity of Mpro. Chemical shift perturbations induced by niacin and hit1indicate a partial overlap of their binding sites, i.e., the catalytic pocket of Mpromay accommodate derivatives with large molecular sizes. Therefore, we searched for drugs containing niacin or hit1pharmacophores and identified carmofur, bendamustine, triclabendazole, and emedastine; these drugs are highly capable of inhibiting protease activity. Our study demonstrates that the fragment-based approach is a feasible strategy for identifying low–molecular-weight drugs against the SARS-CoV-2 and other potential targets lacking specific drugs.
Lack ofKcnn4improves mucociliary clearance in muco-obstructive lung disease
Authors: Vega Génesis , Guequén Anita , Philp Amber R. , Gianotti Ambra , Arzola Lilian , Villalón Manuel , Zegarra-Moran Olga , Galietta Luis J.V. , Mall Marcus A. , Flores Carlos A. 
Published: May 2020

ABSTRACTAirway mucociliary clearance (MCC) is the main mechanism of lung defense keeping the airways free of infection and mucus obstruction. Airways surface liquid (ASL) volume, ciliary beating and mucus are central for proper MCC, and are critically regulated by sodium (Na+) absorption and anion secretion. Impaired MCC is a key feature of muco-obstructive disease. The calcium-activated potassium (K+)channel KCa.3.1, encoded by theKcnn4gene, participates in intestinal ion secretion and previous studies showed that its activation increase Na+absorption in airway epithelia, suggesting that hyperpolarization induced by KCa3.1 was sufficient to drive Na+absorption. However, its role in airway epithelial function is not fully understood. We therefore aimed to elucidate the role of KCa3.1 in MCC in a genetically engineered mouse model. We show that KCa3.1 inhibition reduced Na+absorption in mouse and human airway epithelium. Furthermore, the genetic deletion ofKcnn4enhanced cilia beating frequency (CBF) and MCCex vivoandin vivo.Kcnn4was silenced in theScnn1b-transgenic mouse (Scnn1btg/+), a model of muco-obstructive lung disease triggered by increased epithelial Na+-absorption, leading to improvements in MCC and reduction of Na+-absorption. KCa3.1 deletion did not change the amount of mucus but did reduce mucus adhesion, neutrophil infiltration and emphysema. Our data support that KCa3.1 inhibition attenuated muco-obstructive disease in theScnn1btg/+mice. K+-channel modulation may be a novel therapeutic strategy to treat muco-obstuctive lung diseases.
What am I tipping you for? Customer response to tipping requests at limited-service restaurants
Authors: Karabas Ismail , Orlowski Marissa , Lefebvre Sarah 
Published: May 2020

Purpose
Tipping within the foodservice industry has traditionally been reserved for full-service restaurants. However, there is a growing trend of tip requests at limited-service restaurants, where tipping occurs prior to consuming the product. This research aims to examine the effect of a point-of-sale tip request at limited-service restaurants on return intentions via customer irritation. It also aims to analyze the moderating effects of check amount and perceived deservingness.

Design/methodology/approach
Four online scenario-based experiments were conducted to test the hypotheses. Participants were recruited from MTurk for all experiments (NStudy 1 = 152; NStudy 2 = 296; NStudy 3 = 206; NStudy 4 = 134).

Findings
Studies 1 and 2 suggested a negative impact of presenting a tip request on return intentions, with customer irritation as the underlying mechanism. Study 3 found the indirect effect was significant only when the check amount was low. Study 4 found that perceived deservingness of a tip also moderated this effect; the indirect effect was significant only when customers felt the employee did not deserve a tip. The effect was attenuated when customers felt the employee deserved a tip.

Originality/value
This paper contributes to the underexplored area of tipping behavior in the limited-service context. The findings contrast extant research on voluntary tipping at full-service restaurants, thus advancing theory by suggesting the consequences of tip requests are contextual and providing practical insights to limited-service establishments contemplating whether to begin requesting tips.
Do financial instruments or grants have a bigger effect on SMEs’ access to finance? Evidence from Hungary
Authors: Nyikos Györgyi , Béres Attila , Laposa Tamás , Závecz Gergő 
Published: May 2020

Purpose
The economic situation in Europe is improving, nevertheless in Central and Eastern Europe (CEE) entrepreneurs and small and medium enterprises (SMEs) are still lacking in finances. In this situation, public funding can play an important role. Besides grants, the use of financial instruments (FIs) has become increasingly popular lately in CEE as well. This paper aims to examine the micro-level effects of the different financial tools to understand which type of finance could be most recommended for policymakers in relation to improving access to finance for SMEs, and thus achieving long-term, sustainable economic growth.

Design/methodology/approach
The database used is a panel with firm-years as the units of analysis, the variables contain firm-level characteristics, yearly aggregated information on European Union (EU) subsidies and yearly aggregated information on credits received by the firms. The analyses are done using propensity score matching. The ultimate goal is to show whether the EU funds – grants and FIs – have contributed to the development of the Hungarian post-communist economy at micro level or not.

Findings
The result shows that the use of subsidies has a positive impact on employment, sales and in certain settings on productivity. It is very important to notice, that grants seem to be used effectively. However, the results also show that the provision of the FI holds more direct relevance to advanced productivity. The conclusion is that FIs have more positive impact on the Hungarian economy.

Originality/value
At the time of the programming for the EU 2021-2027 multiannual financial framework, the paper presents original research in the field of access to finance showing evidence and evaluating the effect of using grants versus FIs, emphasiing differences between the two development tools. It is providing an invaluable insight to the policymaker for planning policy tools and use of funds in a most effective and efficient way.
Determinants of Muslim travellers Halal food consumption attitude and behavioural intentions
Authors: Hanafiah Mohd Hafiz , Hamdan Nurul Alia Aqilah 
Published: May 2020

PurposeThe demand for Muslim friendly tourism industry has been gaining momentum from the increasing number of Muslim travellers globally. This paper aims to examine the role of religiosity in Muslim travellers Halal food consumption attitude and behaviour while travelling to the non-Muslim destination.Design/methodology/approachA total of 384 respondents participated in this study through an online survey. This study used the partial least square structural equation modelling to assess the survey measurements and hypotheses testing.FindingsThis study asserts that the Muslim travellers’ Halal consumption attitude relies heavily on their level of religiosity, subjective norms and perceived control behaviour. Besides, the results provide evidence that consumption attitude mediates the relationship between religiosity, subjective norm, perceived behavioural control and intention in consuming Halal food products while travelling abroad.Practical implicationsThis study highlighted the critical aspects of an emerging Muslim market that travel for leisure with the urge to comply with their Islamic teachings and religiosity. It is vital for non-Muslim tourism destination marketers to tailor their marketing strategies and consider promoting Islamic dietary rules when planning their travel packages.Originality/valueThis study is one of the few that focus on Muslim-friendly tourism and the role of religiosity in Muslim traveller’s Halal food consumption behaviour. This study confirms that the theory of planned behaviour model can be used to explain Muslim travellers Halal food consumption attitude while travelling to a non-Muslim destination.
New directions for service research: refreshing the process of theorizing to increase contribution
Authors: Brodie Roderick J. , Peters Linda D. 
Published: May 2020

PurposeFor service research to develop as an applied social science there is the need to refresh the process of theorizing so it focuses not only on increasing new academic knowledge but also on knowledge that is managerially relevant. This paper aims to provide guidelines to achieve this.Design/methodology/approachA theorizing process that integrates general theoretic perspectives and contextual research to develop midrange theory is developed. The process is based on the philosophical foundations of pragmatism and abductive reasoning, which has the origins in the 1950s when the management sciences were being established.FindingsA recent research stream that develops midrange theory about customer and actor engagement is used to illustrate the theorizing process.Practical implicationsPracticing managers, customers and other stakeholders in a service system use theory, so there is a need to focus on how theory is used in specific service contexts and how this research leads to academic knowledge that is managerially relevant. Thus, as applied social science, service research needs to explicitly focus on bridging the theory–praxis gap with midrange theory by incorporating a general theoretic perspective and contextual research.Originality/valueThe contribution comes from providing a broader framework to guide the theorizing process that integrates general theoretic perspectives and applied research to develop midrange theory. While general theories operate at the most abstract level of conceptualization, midrange theories are context-specific and applied theory (theories-in-use) is embedded in empirical research.
A proposal for the role of the arts in a new phase of second-order cybernetics
Authors: Scholte Tom 
Published: May 2020

Purpose
The purpose of this paper is to suggest a more central role for reflexive artistic practices in a clarified research agenda for second-order cybernetics (SOC). This is offered as a way to assist the field in the further development of its theoretical/methodological “core” and, subsequently, enhance its impact on the world.

Design/methodology/approach
The argument begins by reviewing Karl Müller’s account of the failure of SOC to emerge as a mainstream endeavor. Then, Müller’s account is recontextualized within recent developments in SOC that are traced through the Design Cybernetics movement inspired by Ranulph Glanville. This alternate narrative frames a supposedly moribund period as a phase of continuing refinement of the field’s focus upon its “proper object of study,” namely, the observer’s mentation of/about their mentation. The implications of this renewed focus are then positioned within Larry Richard’s vision of the cybernetician, not as “scientist” per se but rather as a “craftsperson in and with time” capable of productively varying the dynamics of their daily interactions. Having centered widespread capacity building for this “craft” as a proposed research agenda for a new phase of SOC, the paper concludes by pointing to the unique and necessary role to be played by the arts in this endeavor. Personal reflections upon the author’s own artistic and theoretical activities are included throughout.

Findings
The development and application of artistic methods for the enhancement of individual capacity for second-order observation is consistent with the purpose of SOC, namely, “to explain the observer to himself.” Therefore, it is in the field’s interest to more fulsomely embrace non-scientific, arts-based forms of research.

Research limitations/implications
In a truly reflexive/recursive fashion, the very idea that first-person, arts-based narratives are seen, from a mainstream scientific point of view, as an insufficiently rigorous form of research is, itself, a research limitation. This highlights, perhaps ironically, the need for cybernetics to continue to pursue its own independent definitions and standards of research beyond the boundaries of mainstream science rather than limiting its own modes of inquiry in the name of “scientific legitimacy.”

Practical implications
A general uptake of the view presented here would expand the horizon of what might be considered legitimate, rigorous and valuable research in the field.

Social implications
The view presented here implies that many valuable contributions that SOC can make to society take place beyond the constraints of academic publication and within the realm of personal growth and social development.

Originality/value
The very clearly defined and “refocused” vision of SOC in this paper can be of substantial utility in developing a more robust, distinctive and concrete research agenda across this field.
Effects of different concentrations of guar, xanthan and locust bean gums on physicochemical quality and rheological properties of corn flour tarhana
Authors: Anil Munir , Durmus Yusuf , Tarakci Zekai 
Published: May 2020

Purpose
Celiac disease patients cannot consume gluten-containing diets; thus, gluten-free products should be offered to meet the nutritional needs of these patients. The purpose of this study was to produce gluten-free tarhana for celiac disease patients using corn flour instead of wheat flour and investigate some physicochemical properties of tarhana. Hydrocolloids were used to compensate for technological deficiencies caused by the absence of gluten.

Design/methodology/approach
Hydrocolloids including guar gum, xanthan gum and locust bean gum were added at concentrations of 0.5% and 1.0% to the corn flour. The substituted corn flour samples were used to produce tarhana powder.

Findings
The pH and acidity measurements were carried out in 0th, 24th and 48th h of fermentation, and for all samples, the pH gradually decreased during fermentation, whereas the acidity increased. According to the color measurements (L, a and b values), it was observed that there was no significant difference (p = 0.588) between the gums in terms of L values in tarhana dough samples. Water retention capacity values of control, guar gum 1%, xanthan gum 1% and locust bean gum 1% were found to be 1.1, 1.1, 0.7 and 1.2 mL/g, respectively. The viscosity measurements were carried out at three different temperatures (30°C, 45°C and 60°C), and the viscosity values were found to decrease significantly (p = 0.000) with the increase in temperature for all the samples studied. The highest viscosity values were obtained by 1.0% xanthan gum (4,333 mPa s) and 0.5% locust bean gum (3,575 mPa s) added tarhana samples for 3 rpm at 30°C. Xanthan gum addition showed the lowest foam capacity values (0.04 mL/mL) among the samples. The samples with guar gum, xanthan gum and locust bean gum are recommended with regard to consistency and foam stability in the production of tarhana.

Originality/value
This study confirms that the use of gums in tarhana, a gluten-free system, is beneficial for the technological aspect. The unfavorable properties that can be seen because of the absence of gluten in corn flour tarhana can be compensated with the use of hydrocolloids, and tarhana can be recommended to celiac disease patients.
Fire safety management of public buildings: a systematic review of hospital buildings in Asia
Authors: Muhamad Salleh Naziah , Agus Salim Nuzaihan Aras , Jaafar Mastura , Sulieman Mohd Zailan , Ebekozien Andrew 
Published: May 2020

PurposeThere is increasing recognition amongst healthcare providers on the necessity to improve fire safety management in healthcare facilities. This is possibly not yet satisfactory because of recent fire incidents in Asia. This paper set out to analyse the literature because of the paucity of systematic reviews on fire safety management of public healthcare facilities and proffer preventive measures.Design/methodology/approachThirty related studies were identified with the support of the Preferred Reporting Items for Systematic reviews and Meta-Analyses via Scopus and Web of Science databases.FindingsInfluencing factors, hindrances to fire safety management and preventive measures for fire-related occurrence in Asian hospital buildings were the three themes that emerged from the reviewed. The factors that influence fire in Asian hospital buildings were categorised into technical, management and legislation factors.Research limitations/implicationsThe recommendations of this paper were based on literature that was systematically reviewed but does not compromise the robustness concerning fire safety management in hospital buildings across Asian countries. Much is needed to be known regarding fire safety in healthcare buildings across Asian countries. This paper recommended exploratory sequential mixed-methods approach as part of the implications for further studies. This will allow in-depth face-to-face interviews and increase the generalisability of future findings concerning fire safety management in hospital buildings across Asian countries to a larger population.Practical implicationsAs part of the practical implications, this paper recommends fire safety management plan as one of the practical possible measures for addressing technical, management and legislation factors. Also recommended is training and fire safety education of healthcare staff in collaboration with safety firefighters to address major issues that may arise from management factors. The government should upgrade the safety technology equipment in healthcare facilities as part of measures to mitigate issues concerning technical and legislation factors. Also, the identified factors are part of the theoretical contributions to the advancement of knowledge and this brings to the front burners new opening.Originality/valueThis is probably the first systematic review paper on fire safety hospital buildings in Asia.