bluuebunny/crossref_metadata_2025_split huggingface dataset:
The combined application of rat bone marrow mesenchymal stem cells and bioceramic materials in the regeneration of dental pulp-like tissues
Authors: Wen Ruixue , Wang Xin , Lu Yongchao , Du Yi , Yu Xijiao 
Published: January 2020

Abstract
Background: To observe the effects of combined application of rat bone marrow mesenchymal stem cells (rBMSCs) and a bioceramic material on pulp-like tissue formation. Methods: Rat incisor root fragments without pulp tissues were prepared and filled with a collagen scaffold seeded with rBMSCs, while one side of the root segment was covered by a bioceramic material (iRoot BP). After culture for 12 h, the root fragments were implanted subcutaneously for 3 months. Hematoxylin and eosin (HE) staining was applied to observe biocompatibility and the formation of pulp-like tissues. Incisor root fragments were divided into three parts (BP1/3 , M1/3, and D1/3) to analyze the area and number of new vessels. Immunohistochemical staining of neuroendocrine marker PGP9.5, dentin sialophosphoprotein ( DSPP ), and vascular endothelial growth factor (VEGF) was applied to observe the formation of pulp-like tissues. Root fragments filled with the collagen scaffold only were used as a control. Results: Three months after implantation, root fragments were collected, which were surrounded by a transparent tissue membrane with a good blood supply. The root fragment cavity was filled with pink vascularized pulp-like tissue. According to HE results, iRoot BP had good biocompatibility with new pulp-like tissues and few infiltrating inflammatory cells. Increases in the number and area of new blood vessels were observed in BP1/3 compared with the other two parts. Expression of PGP9.5 and DSPP showed that the newly formed tissues were similar to normal pulp tissues. Conclusion: iRoot BP has good biocompatibility and increases the number and area of new blood vessels. The combined application of stem cells and bioceramic materials may be a better method for pulp revascularization .
Motivational Interviewing and Self-care in Type 1 Diabetes:a randomized controlled clinical trial study protocol
Authors: Alvarado-Martel Dacil , Boronat Mauro , Alberiche María del Pino , Algara María Andrea , Wägner Ana M 
Published: January 2020

Abstract
BackgroundType 1 diabetes is a chronic disease with complex therapeutic recommendations that require day-to-day lifestyle changes. People with type 1 diabetes need to be involved in their health care in order to achieve satisfactory control of the condition. Motivational Interviewing is a communication tool that has been shown to be effective in changing behaviors in people with addictions, obesity and type 2 diabetes.Our objective is to evaluate the impact of a Motivational Interviewing intervention in patients with type 1 diabetes, through a randomized controlled clinical trial.MethodsSixty-six patients with type 1 diabetes and hemoglobin A1c>= 8% have been included and randomly assigned (computer-generated sequence, sealed envelopes, ratio 1:1) either to the intervention group or to the control group. In the intervention group, appointments every four months with the endocrinologist include Motivational Interviewing; in the control group, the appointments proceed as usual. Patients will be followed for 16 months.The primary outcome will be self-care behaviors, assessed according to a validated questionnaire, the Diabetes Self-Care Inventory-Revised Version. Secondary outcomes include: HbA1c, motivation for self-care, self-efficacy, health-related quality of life, satisfaction with professional-patient relationship, fulfillment of patients' own objectives and other sociodemographic and clinical variables related to disease control.The practitioners will receive training in Motivational Interviewing in order to help them promote adherence to self-care, encourage patient motivation and improve the doctor-patient relationship. The Motivational Interviewing intervention will be evaluated through videorecordings of the sessions and the administration of a purpose-built questionnaire, the EVEM 2.0 scale, by two psychologists, blinded to the assigned treatment. DiscussionThere is evidence that MI can improve self-care in type 2 diabetes. In T1D, however, its application has focused on adolescents, but, at present, there are no published data on the effect of MI in adults with T1D. In this study, we aim to evaluate the effect of MI on self-care and HbA1c.
Overexpression of Sideroflexin1 (SFXN1) Predicts Worse Overall Survival in Lung Adenocarcinoma
Authors: Liu Wenting , Jiang Kaiting , Wang Jingya , Mei Ting , Fan Xuehan , Zhang Yujia , Huang Dingzhi 
Published: January 2020

Abstract
BackgroundMitochondria are the core organelle of cellular energy and metabolism, also closely related to the growth and survival of cancer cells. This research aimed to discover and evaluate a promising prognostic biomarker, a novel mitochondrial gene—Sideroflexin1 (SFXN1), for lung adenocarcinoma (LUAD).ResultsAnalysis of 594 samples from The Cancer Genome Atlas (TCGA) Lung Cancer Cohort, this present study indicated that SFXN1/2/4/5 were overexpressed in LUAD tissue compared with normal lung tissue whereas only the SFXN1 was associated significantly with the overall survival (OS) from the Kaplan-Meier survival analysis (p=0.00093), suggesting that SFXN1 could be a potential prognostic biomarker. Furthermore, the logistic regression of the correlation of clinicopathological features and the expression status of SFXN1 from 522 patients with LUAD in TCGA revealed that the expression level of SFXN1 was related to Eastern Cooperative Oncology Group (ECOG), clinical stage, tumor size and lymph nodes invasion (all p<0.05). Additionally, overexpression of SFXN1 remained associated positively and independently with a worse prognosis after the multivariate Cox regression (HR=1.486, p=0.022). Functionally, SFXN1 involved in the cell cycle, specifically in DNA replication and meiosis, ATPase activity and folate-dependent one-carbon metabolism from the outcomes of Eastern Cooperative Oncology Group (KEGG) and Gene Ontology (GO) enrichment. ConclusionSFXN1 might promote the proliferation and metabolism of cancer cells and function as a prognostic indicator for LUAD, which may contribute to the development of targeted therapy and the emergence of metabolism-based treatment in clinical practice.
Association study of GFAP Polymorphisms with schizophrenia in Han Chinese population
Authors: Yu Wenjuan , he sidi , Yu Yimin , He Shen , Xu Qingqing , Yu Shunying , Huang Jingjing , Li huafang 
Published: January 2020

Abstract
INTRODUCTION Glial fibrillary acidic protein (GFAP) is considered to be an astrocyte activation marker. Alterations in structural and functional molecules expressed by astrocytes may play a role in the pathophysiology of schizophrenia (SCZ). In the present study, the single nucleotide polymorphisms (SNPs) of GFAP were analyzed for association with schizophrenia in Han population. METHODS Three tag SNPs within GFAP were genotyped in 629 SCZ patients and 655 healthy controls in the Han Chinese population.The schizophrenia database(SCZB) was used to investigate whether GFAP is differentially expressed in the brain regions of schizophrenia and healthy control individuals.In addition, expression quantitative trait loci(eQTL) analysis was used to investigate differential GFAP expression between different genotypes. RESULTSIt was shown that the rs3785891 GFAP polymorphism may be associated with schizophrenia ( p =0.04). There were no significant differences in the expression of hippocampal, prefrontal cortex or cortical GFAP between schizophrenia patients and control individuals. Furthermore,the brain eQTL analysis revealed a significant association between the rs3785891 polymorphism and GFAP expression in the intralobular white matter ( p = 0.04), thalamus ( p =0.024), hippocampus ( p =0.011), and occipital cortex ( p =0.0075). CONCLUSIONS This study shows that rs3785891 may be linked to schizophrenia susceptibility in the Han population. Further study is required for clarification the role of genetic variation around these SNPs in expression pattern of the GFAP gene, which may be involved in schizophrenia pathogenesis.
Incidence, bacteriological profile and antibiotic sensitivity pattern of neonatal sepsis in a tertiary health facility in Abuja, North-central Nigeria.
Authors: Oluyinka Olutunde , Airede Kareem I. , Olateju Kudi E. , Stephen Obaro K. , Izevbigie Nosakhare , Iloh Kenechukwu K. , Igbokwe Obianuju , Osuorah Chidiebere Donatus Ignatius 
Published: January 2020

Abstract
Background: Neonatal sepsis is commonly caused by bacteria in the first 28 days of life. If prompt management is not instituted, it could lead to death within hours of onset. Due to diagnostic limitation in developing settings, prompt laboratory identification of causative organism is usually a challenge. To prevent mortality, clear knowledge of bacteria and their antibiotics sensitivity pattern is important for prompt empirical treatment. Methods: This prospective and cross-sectional study enrolled 339 newborns and were admitted for probable sepsis to the special care unit of the university of Abuja Teaching Hospital. Socio-demographic profile and Blood culture was done from every enrolled newborn using BACTEC 9050. The pattern of the clinical features, incidence, bacteriological profile and antibiotic sensitivity pattern of newborns with confirmed neonatal sepsis were documented. Results: A total of 645 newborn were admitted for probable sepsis during the study period based on clinical features and initial laboratory work-up. Forty-six of the 645 newborns (46/645) had laboratory confirmed sepsis based on blood culture resulting in a neonatal sepsis incidence rate of 71.3 (95%CI 50.7-91.9) per 1000 admitted newborn. Seventeen of the 46 confirmed sepsis cases were among the 1322 newborns delivered within the study facility during the study period giving an in-hospital neonatal sepsis incidence rate of 12.9 (95% CI 6.7-19.0) per 1000 live birth. Amongst the 46 babies with positive blood culture, 27/46 (58.7%) had normal white cell count while the remaining 19/46 (41.3%) had abnormal results. In all, 52 counts of bacteria categorized into 11 bacteria species were isolated from the 46 positive blood cultures. Enterococcus spp and streptococcus species were the commonest gram-positive while Escherichia coli and Chryseomonas luteola were the commonest gram-negative bacteria isolates. Imipenem (all bacterial isolates except Vibrio fluvialis), Augmentin (Streptococcus spp, Staphylococcus spp, Escherichia spp, Enterococcus spp, Klebsiella spp, Moraxella catarrhalis, Acinetobacter baumannii), Vancomycin (Streptococcus spp, Staphylococcus spp, Escherichia spp, Enterococcus spp), and Ofloxacin (all except Vibrio fluvialis and Citrobacter freundii) had the widest coverage of bacteria isolated from newborn with sepsis. Conclusion: Sepsis in newborn is still prevalent in our environment and compared to previous documented isolates and sensitivity pattern, the bacteria causes, and their antibiotic sensitivity patterns appears to be changing.
Randomized controlled trial on the effect of Internet-based Cognitive Behavioral Therapy with Real-Time Therapist Support Via a Video Conference for patients with chronic pain: A study protocol
Authors: Taguchi Kayoko , Numata Noriko , Kutsuzawa Kana , Yoshimura Kensuke , Shimizu Eiji 
Published: January 2020

Abstract
Background: Chronic pain, which persists for at least 12 weeks, is a serious medical condition that not only causes numerous physical and mental conditions, but also affects a person's entire life. Previous studies concerning cognitive behavioral therapy for patients with chronic pain have shown low to intermediate effectiveness. This paper describes the study protocol for a randomized controlled trial (RCT) to evaluate the effectiveness of our newly developed Internet-based Cognitive Behavioral Therapy with Real-Time Therapist Support Via a Video Conference (vCBT) as an adjunct to usual care compared with only usual care for chronic pain. Methods: The RCT comprises two parallel groups (vCBT + usual care and usual care alone) of 20 participants each (n=40) who were diagnosed with chronic pain. We aim to evaluate the effectiveness of the intervention over 16 weeks. The primary outcome is pain intensity, which will be assessed by the Numerical Rating Scale (NRS) at week 16. Secondary outcomes are pain catastrophic perception and daily life impairment degree, each assessed by the Pain Catastrophizing Scale (PCS) and Pain Disability Assessment Scale (PADS). In addition, anxiety, depression, and quality of life will be assessed. All measures will be assessed at weeks 1 (baseline), 8 (middle), and 16 (post). Discussion: This study aims to demonstrate the effectiveness of Internet-based CBT with Real-Time Therapist Support Via a Video Conference for patients with chronic pain. The findings of this study will show a feasibility and safety of online CBT for chronic pain, valuable addition to the treatment options for these patients. And we expect that many therapists will be able to universally provide CBT of comparable quality in the treatment of chronic pain by using the CBT protocol which we have developed.
Ethanol in Food affects behaviour and HPA axis activity during development in zebrafish larvae Subhead: Ethanol in Food affects Nerves during development in zebrafish larvae
Authors: du wenxiao , Chen Xiaoli , Zhao Zhenjun , shi min , bian fuhua 
Published: January 2020

Abstract
Long-term alcohol intake from food can lead to numerous mental disorders in humans, and cause serious problems for governments and families worldwide. However, currently, it is unclear how alcohol affects the hypothalamic–pituitary–adrenal (HPA) axis. In the present study, using zebrafish larvae exposed to 1% ethanol, we made Zebrafish behavioural analysis, samples were collected for the enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR) experiments,and made statistical analyses at last. we found that ethanol decreases the locomotor activity of zebrafish larvae,showed a more intense reaction to external stimuli,increases the secretion of HPA axis hormones in zebrafish larvae,influences the secretion of neurotransmitters,alters key gene expression during neurotransmitter metabolism. Ethanol exposure reduced zebrafish locomotor activity, increased their HPA axis activity, and led to significant changes in the secretion of dopamine and serotonin. These findings provide us with a new understanding of the effects of ethanol on the HPA axis.
Uninformed Decision Making and Regret About the Delay in Childbearing Decision Among Japanese Women and Men Seeking Fertility Treatment: A Cross-Sectional Study
Authors: Adachi Tomoko , Endo Masayuki , Ohashi Kazutomo 
Published: January 2020

Abstract
Background: In Japan, mean maternal and paternal ages at first birth have steadily increased over the past two decades, and more and more women and men seek fertility treatment. The aim of this study was to examine regret over the timing of the childbearing decision and reasons for its delay.Methods: This cross-sectional study included 219 women and 169 men referred to fertility facilities in Japan from July to December 2018. Participants completed a questionnaire on the reasons for their delay in childbearing decision and the degree of regret regarding their decision. Multiple linear regression was used to analyze the association between degree of regret and the reasons for the delay.Results: The top three reasons for the delay in childbearing decision in women were “Establishing the relationship,” “Health problems,” and “Financial security.” The top three reasons in men were “Establishing the relationship,” “Financial security,” and “Lack of fertility knowledge.” Lack of fertility knowledge was associated with regret over the timing of the childbearing decision in women (β = 0.232, 95% CI = 0.075-0.318, p = 0.002) and men (β = 0.238, 95% CI = 0.083-0.371, p = 0.002). In men, health problems was also associated with regret over the timing of the childbearing decision (β = 0.196; 95% CI = 0.039-0.332, p = 0.013).Conclusions: Uninformed decision making based on lack of fertility knowledge was significantly associated with regret later in life. It is important for early reproductive-aged women and men to have fertility knowledge in order to make an informed decision about the timing of childbearing to alleviate the possibility of experiencing regret later in life.Keywords: uninformed decision making, regret, delay in childbearing, fertility knowledge, preconception health
Oral prednisolone administration in postoperative period improves results of TKA in patient with severe range of motion limitation – a paired outcome study
Authors: Grzelecki Dariusz , Kowalczewski Jacek , Kordasiewicz Bartłomiej , Sibinski Marcin , Olewnik Łukasz , Marczak Dariusz 
Published: January 2020

Abstract
Background One of the most common problems related to total knee arthroplasty (TKA) is postoperatively limited range of motion, especially in the most difficult cases. The aim of the study was to assess the influence of oral use of prednisolone in the postoperative period on the final results in a patient after TKA with severely limited postoperative range of motion. Methods Thirty-two patients (32 knees) with a mean age of 61.2 years were selected from our prospectively-collected data base with severe knee range of motion limitation (rheumatoid arthritis, psoriasis, ankylosing spondylitis, posttraumatic and postoperative osteoarthritis). Prednisolone was administrated in two doses per day (1 mg per kg of body mass) and then the dose was slowly reduced for no more than three months. Results The prednisolone group demonstrated significantly better results one year after the operation than the non-prednisolone group in terms of range of motion (mean extension 0.5˚ and 3.0˚; p=0.02, mean flexion 100.1˚ and 88˚; p= 0.01), WOMAC (mean 85.0 and 76.8 points; p=0.02) and KSS clinical score (mean 86.9 and 81.7; p=0.001) and KSS functional score (79.3 and 71.0 points; p=0.01). No significant differences were observed between the groups in terms of postoperative systemic and surgical site complications; therefore, no complications could be defined as steroid related. Conclusions The administration of oral prednisolone for a longer postoperative time in patients with severe preoperative range of motion following TKA significantly improves functional results without steroid related complications at one year follow-up.
Levels of Biological Markers of Nitric Oxide in Serum of Patients with Mandible Factures
Authors: Ratajczak-Wrona Wioletta , Wozniak Lukasz , Borys Jan , Antonowicz Bozena , Nowak Karolina , Bortnik Piotr , Jablonska Ewa 
Published: January 2020

Abstract
Background Nitric oxide is a small gaseous molecule with significant bioactivity. It has been observed that NO may have a dual role dependent on its production and concentrations in the bone microenvironment. The objective of the study was to assess the concentration of total nitric oxide malonyldialdehyde, nitrotyrosine, and asymmetric dimethylarginine in the serum of patients with mandibular fractures and to understand the relationship between these compounds, in order to expand the knowledge base of the role of nitric oxide and its activity indicators in the process of bone fracture healing.Material and methods The study included patients with mandibular fractures who were undergoing inpatient and outpatient treatments. Results were analyzed with respect to the measurement time. Total nitric oxide concentration in the blood serum was determined according to the Griess reaction, while the concentration of malonyldialdehyde, nitrotyrosine, and asymmetric dimethylarginine was estimated using the immunoenzymatic method (i.e. enzyme-linked immunosorbent assay).Results Before procedure as well as on the first day and 2 and 6 weeks after the procedure, higher concentrations of total nitric oxide and lower concentrations of malonyldialdehyde were observed in the blood serum of patients with mandibular fractures compared to the control group. No statistically significant differences were found in nitrotyrosine concentrations in the blood serum of patients throughout the measurement period. However, a significantly higher asymmetric dimethylarginine concentration was observed in the patient serum before the procedure and on the first day of operation as compared with the control group. Analysis of the results observed in patient serum with respect to the number of fractures within the mandible demonstrated the same trend of concentrations for the tested compounds for the entire study group.Conclusions In summary, our results revealed that the intensity of local processes resulting from mandibular fractures is associated with the concentration of nitric oxide, confirming its significant role, as well as that of its indicators, in the process of bone fracture healing in this patient population.
Bone mineral density and bone metabolism in postmenopausal women with type 2 diabetes in a Chinese community: a cross-sectional study
Authors: Han Wu , Zhang Yufan , Zhou Wenbin , Dai Jing , Yao Tao , Xu Jingjing , He Wei 
Published: January 2020

Abstract
AMI: There is growing evidence of a complex interaction between T2DM and osteoporosis. The purpose of this study was to further study the relationship between BTMs and fasting blood glucose (FBG) in postmenopausal patients with type 2 diabetes and to analyze the effect of hyperglycemia on bone metabolism. Methods: Six hundred and twelve (612) postmenopausal women were included, including one hundred and seven (107) subjects with T2DM and five hundred and five (505) subjects without diabetes. BMD was measured by DXA (dual-energy X-ray absorptiometry). Markers of bone formation (P1NP) and resorption ( CTX ) were quantified. Results: Compared to controls, postmenopausal women with diabetes had a higher prevalence of previous osteoporosis fracture (27.1% vs. 17.4% for diabetic and nondiabetic women, respectively) and a higher BMD. The P1NP level in women with T2DM was 49.451 ng/ml, while in N-DM individuals, it was 58.633 ng/ml, (p = 0.017). The CTX level in women with T2DM was 0.325 ng/ml, while in N-DM individuals, it was 0.412 ng/ml (p=0.039). In addition, P1NP was significantly negatively associated with age (β=-0.590; p= 0.002) and FBG (β=-1.950; p = 0.035). CTX was negatively associated with FBG (β=-0.029; p = 0.015). Conclusions: T2DM was associated with higher BMD and paradoxically, with an increased risk of fracture. Postmenopausal women with T2DM had lower bone turnover than controls. With increased levels of FBG, bone formation and bone resorption were reduced, and the overall bone turnover level was reduced. Keywords Type 2 diabetes mellitus · Bone mineral density · Bone turnover markers · Osteoporosis fracture