bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustainability at a cost: An inceptive analysis of “extended” fishing ban on the livelihoods of fishers of Tamil Nadu
Authors: Amali Infantina John , Priya Parasuram , Deepak Samuel Vijay Kumar , Abhilash Kottarathil Rajendiran , Purvaja Ramchandran , Ramesh Ramachandran 
Published: February 2020

AbstractAn emphasis on sustainable marine fish production has paved the way for the enactment of Marine Fisheries Regulation Acts and subsequent regulations in India. A closed season ban on fishing has been one of the very few successful regulatory measures since its introduction in 2001 in Tamil Nadu. Since 2017, the fishing ban period has been extended from 45 to 60 days for the east coast of India, for ecosystem‐based marine fisheries management. This study analyses the socioeconomic impacts of change in the fishing ban (closed season) across the four coastal zones of Tamil Nadu. For the past 10 years, CPUE has been stable or increased marginally. Among the four coasts, the relative change in employment and labour income loss was highest for Palk Bay (36.84%). Transaction costs of implementation of the ban caused an additional expense of Rs 496.5 million to the Government in 2017 and the overall total labour income loss has leaped from Rs 1,638.2 to 2,100.2 million due to the extension of the ban. The fishing ban has aided in the recovery of habitat and regeneration of stock through recruitment. Training and capacity building on alternative livelihood options are highly recommended to enable the workers to cope with the ban period.
Lung inflammation in the pathogenesis of rheumatoid arthritis
Authors: Demoruelle M. Kristen , Wilson Timothy M. , Deane Kevin D. 
Published: February 2020

AbstractThe primary manifestation of rheumatoid arthritis (RA) is articular disease; however, extra‐articular disease can also occur. In particular, pulmonary disease is a leading cause of morbidity and mortality in individuals with RA. Herein, we will review the types, prevalence, risk factors, and potential pathophysiology of lung disease in individuals with established RA. We will also discuss the emerging understanding of potential role of the lung in the generation of RA‐related autoantibodies during a period of disease development termed “pre‐RA.” Finally, we will discuss a research agenda outlining the next steps to improve our understanding and management of lung inflammation and lung disease throughout the natural history of RA.
Medication errors in prescription and administration in critically ill patients
Authors: Suclupe Stefanie , Martinez‐Zapata Maria Jose , Mancebo Jordi , Font‐Vaquer Assumpta , Castillo‐Masa Ana María , Viñolas Iris , Morán Indalecio , Robleda Gemma 
Published: February 2020

AbstractAimTo determine the prevalence and magnitude of medication errors and their association with patients' sociodemographic and clinical characteristics and nurses' work conditions.DesignAn observational, analytical, cross‐sectional and ambispective study was conducted in critically ill adult patients.MethodsData concerning prescription errors were collected retrospectively from medical records and administration errors were identified through direct observation of nurses during drug administration. Those data were collected between April and July 2015.ResultsA total of 650 prescription errors were identified for 961 drugs in 90 patients (mean error 7[SD 4.1] per patient) and prevalence of 47.1% (95% CI 44–50). The most frequent error was omission of the prescribed medication. Intensive care unit stay was a risk factor associated with omission error (OR 2.14; 1.46–3.14: p < .01). A total of 294 administration errors were identified for 249 drugs in 52 patients (mean error 6 [SD 6.7] per patient) and prevalence of 73.5% (95% CI 68–79). The most frequent error was interruption during drug administration. Admission to the intensive care unit (OR 0.37; 0.21–0.66: p < .01), nurses’ morning shift (OR 2.15; 1.10–4.18: p = .02) and workload perception (OR 3.64; 2.09–6.35: p < .01) were risk factors associated with interruption.ConclusionsMedication errors in prescription and administration were frequent. Timely detection of errors and promotion of a medication safety culture are necessary to reduce them and ensure the quality of care in critically ill patients.ImpactMedication errors occur frequently in the intensive care unit but are not always identified. Due to the vulnerability of seriously ill patients and the specialized care they require, an error can result in serious adverse events. The study shows that medication errors in prescription and administration are recurrent but preventable. These findings contribute to promote awareness in the proper use of medications and guarantee the quality of nursing care.
Nusinersen treatment and cerebrospinal fluid neurofilaments: An explorative study on Spinal Muscular Atrophy type 3 patients
Authors: Faravelli Irene , Meneri Megi , Saccomanno Domenica , Velardo Daniele , Abati Elena , Gagliardi Delia , Parente Valeria , Petrozzi Lucia , Ronchi Dario , Stocchetti Nino , Calderini Edoardo , D’Angelo Grazia , Chidini Giovanna , Prandi Edi , Ricci Giulia , Siciliano Gabriele , Bresolin Nereo , Comi Giacomo Pietro , Corti Stefania , Magri Francesca , Govoni Alessandra 
Published: February 2020

AbstractThe antisense oligonucleotide Nusinersen has been recently licensed to treat spinal muscular atrophy (SMA). Since SMA type 3 is characterized by variable phenotype and milder progression, biomarkers of early treatment response are urgently needed. We investigated the cerebrospinal fluid (CSF) concentration of neurofilaments in SMA type 3 patients treated with Nusinersen as a potential biomarker of treatment efficacy. The concentration of phosphorylated neurofilaments heavy chain (pNfH) and light chain (NfL) in the CSF of SMA type 3 patients was evaluated before and after six months since the first Nusinersen administration, performed with commercially available enzyme‐linked immunosorbent assay (ELISA) kits. Clinical evaluation of SMA patients was performed with standardized motor function scales. Baseline neurofilament levels in patients were comparable to controls, but significantly decreased after six months of treatment, while motor functions were only marginally ameliorated. No significant correlation was observed between the change in motor functions and that of neurofilaments over time. The reduction of neurofilament levels suggests a possible early biochemical effect of treatment on axonal degeneration, which may precede changes in motor performance. Our study mandates further investigations to assess neurofilaments as a marker of treatment response.
miR24‐2 accelerates progression of liver cancer cells by activating Pim1 through tri‐methylation of Histone H3 on the ninth lysine
Authors: Yang Yuxin , Song Shuting , Meng Qiuyu , Wang Liyan , Li Xiaonan , Xie Sijie , Chen Yingjie , Jiang Xiaoxue , Wang Chen , Lu Yanan , Xin Xiaoru , Pu Hu , Gui Xin , Li Tianming , Xu Jie , Li Jiao , Jia Song , Lu Dongdong 
Published: February 2020

AbstractSeveral microRNAs are associated with carcinogenesis and tumour progression. Herein, our observations suggest both miR24‐2 and Pim1 are up‐regulated in human liver cancers, and miR24‐2 accelerates growth of liver cancer cells in vitro and in vivo. Mechanistically, miR24‐2 increases the expression of N6‐adenosine‐methyltransferase METTL3 and thereafter promotes the expression of miR6079 via RNA methylation modification. Furthermore, miR6079 targets JMJD2A and then increased the tri‐methylation of histone H3 on the ninth lysine (H3K9me3). Therefore, miR24‐2 inhibits JMJD2A by increasing miR6079 and then increases H3K9me3. Strikingly, miR24‐2 increases the expression of Pim1 dependent on H3K9me3 and METTL3. Notably, our findings suggest that miR24‐2 alters several related genes (pHistone H3, SUZ12, SUV39H1, Nanog, MEKK4, pTyr) and accelerates progression of liver cancer cells through Pim1 activation. In particular, Pim1 is required for the oncogenic action of miR24‐2 in liver cancer. This study elucidates a novel mechanism for miR24‐2 in liver cancer and suggests that miR24‐2 may be used as novel therapeutic targets of liver cancer.
Treatment of keratinocyte carcinoma in elderly patients – a review of the current literature
Authors: Leus A.J.G. , Frie M. , Haisma M.S. , Terra J.B. , Plaat B.E.C. , Steenbakkers R.J.H.M. , Halmos G.B. , Rácz E. 
Published: February 2020

AbstractA large percentage of the patients with keratinocyte carcinoma (KC, formerly known as non‐melanoma skin cancer) is of advanced age and often too frail for standard therapies. However, no specific treatment recommendations are given for this population. This review aimed to give an overview of the current literature on the best practice for the treatment of elderly patients with KC. A literature search was performed in MEDLINE, using ‘keratinocyte carcinoma’, ‘elderly’, ‘treatment’ and various synonyms. Case reports, reviews, comments, non‐English literature and studies with a sample size <15 were excluded. After selection, a total of 47 studies were reviewed. Two types of studies were identified, focusing on (I) the effect of age on treatment outcomes and (II) alternative treatment schedules for elderly patients. Studies on surgery, the gold standard, describe larger lesions and defect size in the elderly population. Recurrence rate, complication rate and disease‐specific survival were not affected by age. Depending on the expected morbidity of a suggested (re‐)excision and patient preferences, a conservative watchful waiting policy can be agreed upon as a shared decision. Other common treatment modalities, such as adjuvant radiotherapy, photodynamic therapy and systemic therapy for basal cell carcinoma (BCC), show comparable results in the elderly and younger population. Alternative treatment schedules for elderly patients include primary hypofractionated radiotherapy, which seems effective and well‐tolerated, although research is limited to case series. Additionally, localized and topical treatments seem safe and effective especially for low‐risk tumours. Data are lacking on the efficacy of systemic therapies of metastatic KC in elderly patients. Efficacy of most treatments (with the exception of photodynamic therapy) is not dependent on age. There is need for more research on the efficacy of adjusted treatment modalities, such as hypofractionated radiotherapy and palliative or curative systemic treatment.
Aging exacerbates high‐fat diet‐induced steatohepatitis through alteration in hepatic lipid metabolism in mice
Authors: Ishizuka Kei , Kon Kazuyoshi , Lee‐Okada Hyeon‐Cheol , Arai Kumiko , Uchiyama Akira , Yamashina Shunhei , Yokomizo Takehiko , Ikejima Kenichi 
Published: February 2020

AbstractBackground and AimAging is an independent risk factor for the progression of non‐alcoholic steatohepatitis. Here, we investigated the role of age‐related alterations in fatty acid metabolism in dietary steatohepatitis using lipidomics analysis.MethodsMale 8‐week and 55‐week‐old C57BL/6 J mice were fed a high‐fat diet (HFD) for 8 weeks. The quality and quantity of lipid molecular species in the liver were evaluated using the lipidomics approach.ResultsElder mice fed an HFD developed more severe steatohepatitis than young mice. Oxidative stress and inflammatory cytokines in the liver were exacerbated following HFD feeding in elder mice compared with young mice. In elder mice, de novo fatty acid synthesis was promoted, whereas β oxidation was blunted following HFD feeding, and lipid secretion from the liver was reduced. The expression of sirtuin 1 was not only reduced with age as expected but also significantly decreased due to intake of HFD. In the lipidomics analysis, the concentrations of diacylglycerol and TAG molecular species containing monounsaturated fatty acids were markedly increased following HFD feeding in elder mice compared with young mice. In contrast, the concentration of phosphatidylethanolamine and phosphatidylcholine molecular species containing polyunsaturated fatty acids were remarkably decreased following HFD feeding in elder mice compared with young mice, and the expression of fatty acid desaturase was blunted.ConclusionsAging‐dependent alterations in lipid metabolism under excessive lipid supply most likely enhance hepatic lipotoxicity, thereby exacerbating metabolic steatohepatitis in elderly.
Hurricane exposure and personality development
Authors: Damian Rodica Ioana , Serrano Surizaday , Hill Patrick L. 
Published: February 2020

AbstractObjectiveWe evaluated the role of natural disaster adversity on personality development in the wake of a hurricane, and the moderating role of previous hurricane exposure.MethodWe used a two‐wave longitudinal design and a diverse sample of emerging adults (n = 691; mean age = 22 years; 72% females, 27% European American, 29% Latino, 23% Asian American, 15% African American, 6% Multiracial/Other) who were exposed to one of the most damaging hurricanes on record, though to differing degrees. Immediately after the hurricane, we assessed objective individual‐level hurricane exposure, previous exposure to hurricanes, demographics, socioeconomic status, and Big Five personality traits. One year later, we re‐assessed Big Five personality traits.ResultsUsing latent change models, we found significant individual differences both in participants’ initial levels of personality traits at baseline, as well as in their developmental patterns of change in the year following the hurricane. However, there was no evidence of mean‐level change. Moreover, neither hurricane exposure level, nor its interaction with previous exposure showed statistically significant associations with the rates of change in any personality trait.ConclusionsThe present findings support a stability account, whereby individuals largely maintain their personality dispositions following an adverse life event, in this case a hurricane.
Associations between materials used and work‐related musculoskeletal hand complaints among haemodialysis nurses
Authors: Westergren Eva , Ludvigsen Mette Spliid , Lindberg Magnus 
Published: February 2020

SUMMARYBackgroundOne in every two haemodialysis nurses has reported musculoskeletal complaints concerning their hands, which is twice that reported for hospital nurses in general. It is possible that there is an association between the materials used by haemodialysis nurses and the occurrence of hand complaints.ObjectivesTo examine the association between the type of dialysis machine and disposables used with the occurrence of hand complaints among haemodialysis nurses. To compare occupational risks of developing work‐related musculoskeletal disorders based on the materials used for haemodialysis.DesignCross‐sectional.ParticipantsTwo hundred and eighty‐two nurses working in 27 haemodialysis centres in Sweden participated in a survey, and 19 nurses at five centres were observed during priming procedures.MeasurementsNurses supplied demographic data and answered the Nordic Musculoskeletal Questionnaire. Centre level data regarding machines and disposables used for haemodialysis during the past year were also collected.ResultsThere were no differences in the prevalence of hand complaints based on the type of haemodialysis machines, dialysers or tubing used. There were no differences found in physical exposure to the hands during priming, based on machine type used.ConclusionThe results of this study could not reveal any association between disposable materials used and the occurrence of hand complaints among haemodialysis nurses. Additionally, there were no occupational risks detected based on the types of machines used. Hence, the results of the present study strongly indicate that a deeper ergonomic analysis of the work environment is needed to understand the prevalence of hand complaints among nurses working in haemodialysis settings.
Cell-based, animal and H1 receptor binding studies relative to the sedative effects of ketotifen and norketotifen atropisomers
Authors: Feng Feifei , Fawcett J Paul , Zhang Hu , Tucker Ian G 
Published: February 2020

Abstract

Objectives
Ketotifen (K) and its active metabolite norketotifen (N) exist as optically active atropisomers. They both have antihistaminic and anti-inflammatory properties but the S-atropisomer of N (SN) causes less sedation than K and RN in rodents. This study investigated whether this could be related to a lower concentration of SN in brain or a lower affinity of SN for rat brain H1 receptors.

Methods
Ketotifen and norketotifen atropisomers were quantified using a validated chiral HPLC assay. RBE4 and Caco-2 cell monolayers were used in uptake and permeability studies, respectively. Free and total brain-to-plasma (B/P) ratios were determined after injecting racemic K and N into rat tail veins. Affinity for rat brain H1 receptors (KI) was determined using the [3H]mepyramine binding assay.

Key findings
Uptake and permeation studies indicate no stereoselective transport for K or N. B/P ratios reveal the brain concentration of N is lower than K with no stereoselective transport into brain. Finally, the [3H]mepyramine binding assay shows SN has the lowest affinity for rat brain H1 receptors.

Conclusion
The lower sedative effect of SN in rodents is probably due to a combination of a lower uptake of N than K into the brain and less affinity of SN for CNS H1 receptors.
Effect of replacing fish meal protein by crystalline amino acid and soy protein concentrate on growth, feed utilization, and metabolism of tambaqui Colossoma macropomum juveniles
Authors: Martins Graciela P. , Mazini Bruno S. M. , Campos Milena A. F. , Oliveira Denise S. , Guimarães Igo G. 
Published: February 2020

AbstractThis research aimed to determine the upper limit level of crystalline amino acid (CAAs) inclusion in tambaqui nutrition. Additionally, we investigated the possibility of total replacement of fish meal (FM) by soy protein concentrate (SPC). An FM‐based control diet was formulated to meet the known nutrient requirements of tambaqui, and three additional diets were prepared by replacing FM protein with 250, 500, and 750 g/kg nitrogen of CAA mixture. The fifth diet was formulated to completely replace the FM by SPC. A growth and a digestibility trial were performed in a completely randomized design with five treatments and three and four replicates, respectively. The optimum replacement level of FM protein by CAA was 280 g/kg based on daily weight gain (DWG). The apparent digestibility coefficients of the nutrients were higher in fish fed the SPC diet than the fish fed the FM diet. The whole‐body lipid and adiposity significantly declined with the increase of CAA in tambaqui diets. Protein and amino acid retention of tambaqui linearly declined with the increase of CAA. Blood chemistry metabolites were not affected by CAA inclusion. We concluded that the SPC could replace FM in tambaqui diets without affecting growth and nutrient digestibility. CAAs could replace FM intact protein up to 250 g/kg (or 97 g/kg inclusion level) without showing any adverse effect on growth and metabolism of tambaqui.
Local adaptation across a complex bioclimatic landscape in two montane bumble bee species
Authors: Jackson Jason M. , Pimsler Meaghan L. , Oyen Kennan J. , Strange James P. , Dillon Michael E. , Lozier Jeffrey D. 
Published: February 2020

AbstractUnderstanding evolutionary responses to variation in temperature and precipitation across species ranges is of fundamental interest given ongoing climate change. The importance of temperature and precipitation for multiple aspects of bumble bee (Bombus) biology, combined with large geographic ranges that expose populations to diverse environmental pressures, make these insects well‐suited for studying local adaptation. Here, we analyzed genome‐wide sequence data from two widespread bumble bees, Bombus vosnesenskii and Bombus vancouverensis, using multiple environmental association analysis methods to investigate climate adaptation across latitude and altitude. The strongest signatures of selection were observed in B. vancouverensis, but despite unique responses between species for most loci, we detected several shared responses. Genes relating to neural and neuromuscular function and ion transport were especially evident with respect to temperature variables, while genes relating to cuticle formation, tracheal and respiratory system development, and homeostasis were associated with precipitation variables. Our data thus suggest that adaptive responses for tolerating abiotic variation are likely to be complex, but that several parallels among species can emerge even for these complex traits and landscapes. Results provide the framework for future work into mechanisms of thermal and desiccation tolerance in bumble bees and a set of genomic targets that might be monitored for future conservation efforts.
Selective transmission of the seedling yellows‐type genotypic variants of Citrus tristeza virus by Aphis gossypii in northern Iran
Authors: Pourhamedi Saeedeh , Barzegar Ali , Rahimian Heshmatollah 
Published: February 2020

AbstractCitrus tristeza virus (CTV) has existed in northern Iran for more than five decades. The long‐time interaction of different virus genotypes with Aphis gossypii, as the only aphid vector of CTV in northern Iran, has led to the emergence of highly virulent subpopulations, among others, in the established foci. Here, we studied the population structure of the originally established CTV isolates present in Satsuma mandarin (Citrus unshiu) trees imported from Japan, and subisolates thereof, formed following experimental transmission by A. gossypii, as well as those evolved through natural transmission by this aphid species in the groves. Symptoms of the naturally spread and the experimentally aphid‐transmitted isolates were similar to those of the Satsuma CTV source isolates for all indicator plants except for sour orange (Citrus aurantium) and grapefruit (Citrus paradisi), with the aphid‐transmitted isolates additionally inducing severe seedling yellows and stunting in these two indicators. Studies on the population heterogeneity of these isolates through comparison of their single‐strand conformational polymorphism profiles and nucleotide sequences of the 25 kDa capsid protein gene from the predominant haplotypes, and dot‐blot hybridization signals, revealed the presence of two major T36‐ and SY568‐ (or NUagA‐) like genotypes along with a minor poorly characterized one in the originally infected Satsuma trees; in contrast, only a certain genomic variant having the highest similarity to the isolate SY568 (and NUagA) was predominant both in the naturally infected trees and in those infected experimentally by A. gossypii. It seems that transmission by A. gossypii to sweet orange (Citrus sinensis) has led to the preponderance of the CTV genomic variants inducing severe seedling yellows in northern Iran.