bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reflectarray antennas: a smart solution for new generation satellite mega-constellations in space communications
Authors: Imaz-Lueje Borja , Prado Daniel R. , Arrebola Manuel , Pino Marcos R. 
Published: December 2020

AbstractOne of the most ambitious projects in communications in recent years is the development of the so-called satellite mega-constellations. Comprised of hundreds or thousands of small and low-cost satellites, they aim to provide internet services in places without existing broadband access. For the antenna subsystem, reflectarrays have been proposed as a cheap solution due to their low profile and manufacturing costs, while still providing good performance. This paper presents a full design of a reflectarray antenna for mega-constellation satellites with a shaped-beam isoflux pattern for constant power flux in the surface of the Earth. A unit cell consisting of two stacked rectangular microstrip patches backed by a ground plane is employed, providing more than 360° of phase-shift. The generalized intersection approach optimization algorithm is employed to synthesize the required isoflux pattern in a 2 GHz bandwidth in Ku-band. To that purpose, a full-wave electromagnetic analysis is employed for the wideband design. The optimized reflectarray layout complies with the specifications of the isoflux pattern in the frequency band 16 GHz–18 GHz, demonstrating the capabilities of this type of antenna to provide a low-cost, low-profile solution for the user beam segment, including different types of shaped beams.
BEX2 suppresses mitochondrial activity and is required for dormant cancer stem cell maintenance in intrahepatic cholangiocarcinoma
Authors: Tamai Keiichi , Nakamura-Shima Mao , Shibuya-Takahashi Rie , Kanno Shin-Ichiro , Yasui Akira , Mochizuki Mai , Iwai Wataru , Wakui Yuta , Abue Makoto , Yamamoto Kuniharu , Miura Koh , Mizuma Masamichi , Unno Michiaki , Kawamura Sadafumi , Sato Ikuro , Yasuda Jun , Yamaguchi Kazunori , Sugamura Kazuo , Satoh Kennichi 
Published: December 2020

AbstractCancer stem cells (CSCs) define a subpopulation of cancer cells that are resistant to therapy. However, little is known of how CSC characteristics are regulated. We previously showed that dormant cancer stem cells are enriched with a CD274lowfraction of cholangiocarcinoma cells. Here we found that BEX2 was highly expressed in CD274lowcells, and that BEX2 knockdown decreased the tumorigenicity and G0phase of cholangiocarcinoma cells. BEX2 was found to be expressed predominantly in G0phase and starvation induced the USF2 transcriptional factor, which induced BEX2 transcription. Comprehensive screening of BEX2 binding proteins identified E3 ubiquitin ligase complex proteins, FEM1B and CUL2, and a mitochondrial protein TUFM, and further demonstrated that knockdown of BEX2 or TUFM increased mitochondria-related oxygen consumption and decreased tumorigenicity in cholangiocarcinoma cells. These results suggest that BEX2 is essential for maintaining dormant cancer stem cells through the suppression of mitochondrial activity in cholangiocarcinoma.
Late Quaternary range shifts of marcescent oaks unveil the dynamics of a major biogeographic transition in southern Europe
Authors: Vila-Viçosa Carlos , Gonçalves João , Honrado João , Lomba Ângela , Almeida Rubim S. , Vázquez Francisco Maria , Garcia Cristina 
Published: December 2020

AbstractMarcescent forests are ecotones distributed across southern Europe that host increased levels of biodiversity but their persistence is threatened by global change. Here we study the range dynamics of these forests in the Iberian Peninsula (IP) during the Late Quaternary, a period of profound climate and anthropic changes. We modeled and compared the distribution of eight oak taxa for the present and two paleoclimatic environments, the Last Glacial Maximum (LGM, ~ 21 kya) and the Mid-Holocene (MH, ~ 6 kya). Presence records were combined with bioclimatic and topographic data in an ensemble modelling framework to obtain spatial projections for present and past conditions across taxa. Substantial distribution shifts were projected between the three studied periods, that were explained by precipitation, winter cold and terrain ruggedness. Results were congruent with paleoclimatic records of the IP and showed that range shifts of these contact zones concurred with range dynamics of both Submediterranean and Temperate oaks. Notably, the distribution ranges of hybrid oaks and marcescent forests matched throughout the late Quaternary. This study contributes to unveil the complex Late-Quaternary biogeography of the ecotone belt occupied by marcescent forests and, more broadly, of Mediterranean oaks. Improved knowledge of species’ responses to climate dynamics will allow us to anticipate and manage future range shifts driven by climate change.
Phage lysin that specifically eliminates Clostridium botulinum Group I cells
Authors: Zhang Zhen , Lahti Meeri , Douillard François P. , Korkeala Hannu , Lindström Miia 
Published: December 2020

AbstractClostridium botulinum poses a serious threat to food safety and public health by producing potent neurotoxin during its vegetative growth and causing life-threatening neuroparalysis, botulism. While high temperature can be utilized to eliminate C. botulinum spores and the neurotoxin, non-thermal elimination of newly germinated C. botulinum cells before onset of toxin production could provide an alternative or additional factor controlling the risk of botulism in some applications. Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the concentration of 5 µM, but shows little or no lytic activity against C. botulinum Group II or III or other Firmicutes strains. CBO1751 is active at pH from 6.5 to 10.5. The lytic activity of CBO1751 is tolerant to NaCl (200 mM), but highly susceptible to divalent cations Ca2+ and Mg2+ (50 mM). CBO1751 readily and effectively eliminates C. botulinum during spore germination, an early stage preceding vegetative growth and neurotoxin production. This is the first report of an antimicrobial lysin against C. botulinum, presenting high potential for developing a novel antibotulinal agent for non-thermal applications in food and agricultural industries.
Chimeric flavivirus enables evaluation of antibodies against dengue virus envelope protein in vitro and in vivo
Authors: Kurosu Takeshi , Hanabara Keiko , Asai Azusa , Pambudi Sabar , Phanthanawiboon Supranee , Omokoko Magot Diata , Ono Ken-ichiro , Saijo Masayuki , Ramasoota Pongrama , Ikuta Kazuyoshi 
Published: December 2020

AbstractIn a secondary dengue virus (DENV) infection, the presence of non-neutralizing antibodies (Abs), developed during a previous infection with a different DENV serotype, is thought to worsen clinical outcomes by enhancing viral production. This phenomenon is called antibody-dependent enhancement (ADE) of infection, and it has delayed the development of therapeutic Abs and vaccines against DENV, as they must be evaluated for the potential to induce ADE. Unfortunately, limited replication of DENV clinical isolates in vitro and in experimental animals hinders this evaluation process. We have, therefore, constructed a recombinant chimeric flavivirus (DV2ChimV), which carries premembrane (prM) and envelope (E) genes of type 2 DENV (DENV-2) R05-624 clinical (Thai) isolate in a backbone of Japanese encephalitis virus (Nakayama strain). DENV E-protein is the most important viral target, not only for neutralizing Abs, but also for infection-enhancing Abs. In contrast to DENV-2 R05-624, DV2ChimV replicated efficiently in cultured mammalian cells and was lethal in interferon-α/β–γ-receptor double-knockout mice. With DV2ChimV, we were able to perform neutralization assays, in vitro and in vivo ADE assays, and in vivo protection assays. These results suggest that the chimeric virus is a powerful tool for evaluation of Abs against DENV.
Effect of straw and inhibitors on the fate of nitrogen applied to paddy soil
Authors: Yu Chunxiao , Xie Xueshi , Yang Hengzhe , Yang Lijie , Li Wentao , Wu Kaikuo , Zhang Weiming , Feng Chen , Li Dongpo , Wu Zhijie , Zhang Lili 
Published: December 2020

AbstractA pot experiment was used to explore the distribution of fertilizer N and agronomic effects in a paddy soil-rice (Oryza sativa L.) system. Five treatments were set: without nitrogen, straw and inhibitor (C), urea (U), urea + straw (US), urea + urease + nitrification inhibitor (UI) and urea + urease + nitrification inhibitor + straw (UIS). Soil and urea-derived microbial biomass N increased significantly in US and UIS compared with straw-free treatments at seedling and tillering, indicating that biotic process play an important role in the retention of fertilizer N with straw addition. About 10% urea-N was recovered as fixed ammonium (FA) at seedling stage, subsequently released at tillering and maturation regardless of treatments, which emphasizes the importance of FA in the retention and supply of fertilizer N in paddy soils. Compared with U, rice yield and N uptake in US decreased by 7.8% and 25.2% respectively, while inhibitors (UIS) alleviated the reduction by 16.4% and 31.6%. The current research indicated UIS is recommended as the most appropriate management strategy in paddy soils of Northeast China based on N dynamics. But the economic effect as well as the field-scale validation need to be further evaluated.
Microbubble formulation influences inflammatory response to focused ultrasound exposure in the brain
Authors: McMahon Dallan , Lassus Anne , Gaud Emmanuel , Jeannot Victor , Hynynen Kullervo 
Published: December 2020

AbstractFocused ultrasound and microbubble (FUS + MB)-mediated blood–brain barrier (BBB) permeability enhancement can facilitate targeted brain-drug delivery. While controlling the magnitude of BBB permeability enhancement is necessary to limit tissue damage, little work has attempted to decouple these concepts. This work investigated the relationship between BBB permeability enhancement and the relative transcription of inflammatory mediators 4 h following sonication. Three microbubble formulations, Definity, BG8774, and MSB4, were compared, with the dose of each formulation normalized to gas volume. While changes in the transcription of key proinflammatory mediators, such as Il1b, Ccl2, and Tnf, were correlated to the magnitude of BBB permeability enhancement, these correlations were not independent of microbubble formulation; microbubble size distribution may play an important role, as linear regression analyses of BBB permeability magnitude versus differential gene expression for these proinflammatory mediators revealed significantly greater slopes for MSB4, a monodisperse microbubble with mean diameter of 4 μm, compared to Definity or BG8774, both polydisperse microbubbles with mean diameters below 2 μm. Additionally, the function of an acoustic feedback control algorithm, based on the detection threshold of ultraharmonic emissions, was assessed. While this control strategy was effective in limiting both wideband emissions and red blood cell extravasation, microbubble formulation was found to influence the magnitude of BBB leakage and correlations to acoustic emissions. This work demonstrates that while the initial magnitude of FUS + MB-mediated BBB permeability enhancement has a clear influence on the subsequent inflammatory responses, microbubble characteristics influence these relationships and must also be considered.
Long non-coding RNA levels can be modulated by 5-azacytidine in Schistosoma mansoni
Authors: Amaral Murilo S. , Maciel Lucas F. , Silveira Gilbert O. , Olberg Giovanna G. O. , Leite João V. P. , Imamura Lucas K. , Pereira Adriana S. A. , Miyasato Patricia A. , Nakano Eliana , Verjovski-Almeida Sergio 
Published: December 2020

AbstractSchistosoma mansoni is a flatworm that causes schistosomiasis, a neglected tropical disease that affects more than 200 million people worldwide. There is only one drug indicated for treatment, praziquantel, which may lead to parasite resistance emergence. The ribonucleoside analogue 5-azacytidine (5-AzaC) is an epigenetic drug that inhibits S. mansoni oviposition and ovarian development through interference with parasite transcription, translation and stem cell activities. Therefore, studying the downstream pathways affected by 5-AzaC in S. mansoni may contribute to the discovery of new drug targets. Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides with low or no protein coding potential that have been involved in reproduction, stem cell maintenance and drug resistance. We have recently published a catalog of lncRNAs expressed in S. mansoni life-cycle stages, tissues and single cells. However, it remains largely unknown if lncRNAs are responsive to epigenetic drugs in parasites. Here, we show by RNA-Seq re-analyses that hundreds of lncRNAs are differentially expressed after in vitro 5-AzaC treatment of S. mansoni females, including intergenic, antisense and sense lncRNAs. Many of these lncRNAs belong to co-expression network modules related to male metabolism and are also differentially expressed in unpaired compared with paired females and ovaries. Half of these lncRNAs possess histone marks at their genomic loci, indicating regulation by histone modification. Among a selected set of 8 lncRNAs, half of them were validated by RT-qPCR as differentially expressed in females, and some of them also in males. Interestingly, these lncRNAs are also expressed in other life-cycle stages. This study demonstrates that many lncRNAs potentially involved with S. mansoni reproductive biology are modulated by 5-AzaC and sheds light on the relevance of exploring lncRNAs in response to drug treatments in parasites.
Lack of PTEN in osteocytes increases circulating phosphate concentrations by decreasing intact fibroblast growth factor 23 levels
Authors: Kawai Masanobu , Kinoshita Saori , Ozono Keiichi , Michigami Toshimi 
Published: December 2020

AbstractFibroblast growth factor 23 (FGF23) has been centric to the regulation of phosphate (Pi) metabolism; however, the regulatory network of FGF23 in osteocytes has not yet been defined in detail. We herein investigated the role of PTEN (phosphatase and tensin homolog deleted from chromosome 10) in this regulation. We created mice lacking PTEN expression mainly in osteocytes by crossingPten-flox mice withDmp1-Cre mice. The lack of PTEN in the osteocytes of these mice was associated with decreased skeletal and serum intact FGF23 levels, which, in turn, resulted in reductions of urinary Pi excretion and elevations of serum Pi levels. Mechanistically, the knockdown of PTEN expression in osteoblastic UMR106 cells activated the AKT/mTORC1 (mechanistic target of rapamycin complex 1) pathway and this was associated with reductions inFgf23expression. Furthermore, the suppression ofFgf23expression by PTEN knockdown or insulin simulation in UMR106 cells was partially restored by the treatment with the mTORC1 inhibitor, rapamycin. These results suggest that FGF23 expression in osteoblastic cells is in part regulated through the AKT/mTORC1 pathway and provide new insights into our understanding of the regulatory network of Pi metabolism.
Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
Authors: Larzábal Mariano , Da Silva Wanderson Marques , Multani Anmol , Vagnoni Lucas E. , Moore Dadin P. , Marin Maia S. , Riviere Nahuel A. , Delgado Fernando O. , Vilte Daniel A. , Victorica Matias Romero , Ma Tao , Le Guan Luo , Talia Paola , Cataldi Angel , Cobo Eduardo R. 
Published: December 2020

AbstractThe zoonotic enterohemorrhagicEscherichia coli(EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance ofNegativibacillusandErysipelotrichaceaein mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.
Deoiled sunflower seeds ameliorate depression by promoting the production of monoamine neurotransmitters and inhibiting oxidative stress
Authors: Lu Xiaomeng , Ce Qi , Jin Long , Zheng Jie , Sun Mei , Tang Xue , Li Duo , Sun Jin 
Published: December 2020

SFS may alleviate depression by increasing aromatic neurotransmitters, improving oxidative stress and inflammation and regulating metabolism.
A large-area, flexible, high contrast and long-life stable solid-state electrochromic device driven by an anion-assisted method
Authors: Wang Lebin , Zhang Xiang , Chen Xi , Li Xiaobai , Zhao Yingming , Li Wenjie , Zhao Jiupeng , Chen Zhiyong , Li Yao 
Published: December 2020

The H2O content in Li+ electrolyte can regulate the EC properties of NiO and ECDs. Large area flexible ECDs are investigated, exhibiting high optical modulation and long-life stability.
Identification of immune-based prostate cancer subtypes using mRNA expression
Authors: Song Jukun , Wang Wei , Yuan Yiwen , Ban Yong , Su Jiaming , Yuan Dongbo , Chen Weihong , Zhu Jianguo 
Published: December 2020

Abstract
Immune infiltration in Prostate Cancer (PCa) was reported to be strongly associated with clinical outcomes. However, previous research could not elucidate the diversity of different immune cell types that contribute to the functioning of the immune response system. In the present study, the CIBERSORT method was employed to evaluate the relative proportions of immune cell profiling in PCa samples, adjacent tumor samples and normal samples. Three types of molecular classification were identified in tumor samples using the ‘CancerSubtypes’ package of the R software. Each subtype had specific molecular and clinical characteristics. In addition, functional enrichment was analyzed in each subtype. The submap and Tumor Immune Dysfunction and Exclusion (TIDE) algorithms were also used to predict clinical response to the immune checkpoint blockade. Moreover, the Genomics of Drug Sensitivity in Cancer (GDSC) database was employed to screen for potential chemotherapeutic targets for the treatment of PCa. The results showed that Cluster I was associated with advanced PCa and was more likely to respond to immunotherapy. The findings demonstrated that differences in immune responses may be important drivers of PCa progression and response to treatment. Therefore, this comprehensive assessment of the 22 immune cell types in the PCa Tumor Environment (TEM) provides insights on the mechanisms of tumor response to immunotherapy and may help clinicians explore the development of new drugs.
The nature of VX Sagitarii
Authors: Tabernero H. M. , Dorda R. , Negueruela I. , Marfil E. 
Published: December 2020

Aims. We present a spectroscopic analysis of the extremely luminous red star VX Sgr based on high-resolution observations combined with AAVSO light curve data. Given the puzzling characteristics of VX Sgr, we explore three scenarios for its nature: a massive red supergiant (RSG) or red hypergiant (RHG), a Thorne Żytkow object, and an extreme asymptotic giant branch (AGB) star.
Methods. Sampling more than one whole cycle of photometric variability, we derive stellar atmospheric parameters by using state-of-the-art PHOENIX atmospheric models. We compare them to optical and near-infrared spectral types. We report on some key features due to neutral elemental atomic species such as Li I, Ca I, and Rb I.
Results. We provide new insights into its luminosity, its evolutionary stage, and its pulsation period. Based on all the data, there are two strong reasons to believe that VX Sgr is some sort of extreme AGB star. Firstly, it has Mira-like behaviour during active phases. VX Sgr shows light variation with amplitude that is much larger than any known RSG and clearly larger than all RHGs. In addition, it displays Balmer line emission and, as shown here for the first time, line doubling of its metallic spectrum at maximum light, both characteristics typical of Miras. Secondly, unlike any known RSG or RHG, VX Sgr displays strong Rb I lines. In addition to the photospheric lines that are sometimes seen, it always shows circumstellar components whose expansion velocity is compatible with that of the OH masers in the envelope, demonstrating a continuous enrichment of the outer atmosphere with s-process elements, a behaviour that can only be explained by a third dredge-up during the thermal pulse phase.
Visualizing sporadic E using aeronautical navigation signals at VHF frequencies
Authors: Hosokawa Keisuke , Kimura Kotaku , Sakai Jun , Saito Susumu , Tomizawa Ichiro , Nishioka Michi , Tsugawa Takuya , Ishii Mamoru 
Published: December 2020

To understand the dynamical properties of sporadic E layer (Es), we have developed a method to visualize the two-dimensional spatial structure of Es by monitoring the occurrence of anomalous propagation of VHF waves used for aeronautical navigation systems in combination with the electron density disturbance index ROTI, that is the Rate of total electron content (TEC) Index obtained from ground-based GPS receivers. We introduce a case of strong Es layer which occurred for ~4 h during daytime of July 4, 2019. In this interval, we succeeded in imaging the structure of Es elongating more in the east–west direction and moving northward gradually. This result indicates that the combination of network observations of aeronautical navigation signals and ROTI is capable of imaging the 2D structure of Es in a wide area including the sea surface, which enables us to discuss the dynamical characteristics and generation mechanism of Es.
Recent Advancement of Synthesis of Isatins as a Versatile Pharmacophore: A review
Authors: Kumar Gajendra , Singh Netra Pal , Kumar Kaushal 
Published: December 2020

AbstractIsatin (1 H-indole-2, 3-Dione) and its derivatives are versatile compounds which acts as a precursor for a large number of pharmacologically active compounds. Therefore isatins have a significant importance in the synthesis of different heterocyclic compounds. Isatins show variety of biological activities. In this review we focus on synthetic methods of isatins and their biological activities such as antimicrobial, anticonvulsant, anti-inflammatory and analgesic activity, antitubercular activity.