bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane
Authors: Stevens Vincent , Thijs Sofie , Vangronsveld Jaco 
Published: December 2020

Abstract
BackgroundA diverse community of microbes naturally exists on the phylloplane, the surface of leaves. It is one of the most prevalent microbial habitats on earth and bacteria are the most abundant members, living in a community that is highly dynamic. While culture-independent approaches greatly increased our knowledge of microbial communities such as the phylloplane, one of the challenges for microbiologists today remains to develop strategies to culture the vast diversity of microorganisms.ResultsWe isolated bacteria from the phylloplane of Hedera helix (common ivy), a widespread evergreen that constitutes an excellent model for studying the phylloplane in the field, using the growth media LB, LB01, YMA, YFlour and YEx. We also included a comparison with the uncultured phylloplane, which contained the highest intra-sample (alpha) diversity. Inter-sample (beta) diversity shifts from LB and LB01 containing the highest amount of resources to YMA and YFlour which are more selective, and YEx which is more limited but also more varied in resources. We show the H. helix phylloplane is dominated by Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes. Further, all growth media more or less equally favored Actinobacteria and Gammaproteobacteria, whereas Bacteroidetes could only be found on LB01, YEx and YMA. LB and LB01 greatly favored Firmicutes and YFlour was most selective for Betaproteobacteria. At genus level, LB favored the growth of Bacillus and Stenotrophomonas, while YFlour was most selective for Burkholderia and Curtobacterium. The in vitro plant growth promotion (PGP) profile that we obtained by testing 200 isolates constitutes an important first step to find candidates with advantageous traits within microbe-assisted approaches. Our isolation effort also resulted in a significant collection of bacterial strains underrepresented in public databases, mostly from the phylum Actinobacteria.ConclusionsThis study contributes as a case study of bacterial culturability including an evaluation of five different growth media, a comparison with the uncultured H. helix phylloplane community and its relation with functional characteristics such as PGP potential which help us to understand the putative ecological and functional role of microbial members living in the phylloplane.
Inhibition of Mitochondrial Carrier Homolog 2 (MTCH2) Suppresses Tumor Invasion and Enhances Sensitivity to Temozolomide in Malignant Glioma
Authors: Yuan Qiuyun , Yang Wanchun , Zhang Shuxin , Li Tengfei , Zuo Mingrong , Zhou Xingwang , Li Junhong , Li Mao , Xia Xiaoqiang , Chen Mina , Liu Yanhui 
Published: December 2020

Abstract
Background: Malignant glioma exerts a metabolic shift from oxidative phosphorylation (OXPHOs) to aerobic glycolysis, with suppressed mitochondrial functions. This phenomenon offers a proliferation advantage to tumor cells and decrease mitochondria-dependent cell death. However, the underlying mechanism for mitochondrial dysfunction in glioma is not well elucidated. MTCH2 is a mitochondrial outer membrane protein that regulates mitochondrial metabolism and related cell death. This study aims to clarify the role of MTCH2 in glioma.Methods: Bioinformatic analysis from TCGA and CGGA databases were used to investigate the association of MTCH2 with glioma malignancy and clinical significance. The expression of MTCH2 was verified from clinical specimens using real-time PCR and western blots in our cohorts. siRNA-mediated MTCH2 knockdown were used to assess the biological functions of MTCH2 in glioma progression, including cell invasion and temozolomide-induced cell death. Biochemical investigations of mitochondrial and cellular signaling alternations were performed to detect the mechanism by which MTCH2 regulates glioma malignancy.Results: Bioinformatic data from public database and our cohort showed that MTCH2 expression was closely associated with glioma malignancy and poor patient survival. Silencing of MTCH2 expression impaired cell migration/invasion and enhanced temozolomide sensitivity of human glioma cells. Mechanistically, MTCH2 knockdown may increase mitochondrial OXPHOs and thus oxidative damage, decreased migration/invasion pathways, and repressed pro-survival AKT signaling.Conclusion: Our work establishes the relationship between MTCH2 expression and glioma malignancy, and provides a potential target for future interventions.
The association between retinal vessel abnormalities and H-type hypertension
Authors: Huang Kuankuan , Zhang Zhixiang , Huang Shan , Jia Yanwen , Zhang Min , Yun Wenwei 
Published: December 2020

Abstract
Background: This study aimed to investigate the relationship between H-type hypertension and retinal vessel abnormalities.Methods: Hypertensive patients were retrospectively enrolled in this study. According to plasma homocysteine (HCY), patients were divided into isolated hypertension and H-type hypertension groups. The diameter of retinal vessels and retinopathy were evaluated by retinal fundus photography. The differences of retinal vessel abnormalities between H-type hypertension and isolated hypertension were investigated by univariate and multivariate regression.Results: A total of 191 hypertensive patients were included, of which 86 were with isolated hypertension and 105 with H-type hypertension. The H-type hypertension group had a higher ratio of retinopathy(P=0.004) and higher degree of retinal arteriosclerosis (P=0.005) than the isolated hypertension group. CRAE (107.47±13.99mm vs. 113.49±11.72mm, P=0.002) and AVR (0.55±0.06 vs. 0.58±0.06, P=0.001) were smaller in H-type hypertension group than those in isolated hypertension group. Multivariate analysis showed that after adjusting for age, sex, course of hypertension and diabetes, H-type hypertension was an independent risk factor of retinopathy (OR, 2.259; 95%CI, 1.165—4.378; P=0.016), CRAE (β=-5.669; 95%CI, -9.452—-1.886; P=0.004), and AVR (β=-0.023; 95%CI, -0.039—-0.007; P=0.005).Conclusion: H-type hypertension is closely related to more retinal vessel abnormalities than isolated hypertension. Controlling H-type hypertension may reduce the risk of small vascular damage.
Internal fixation of anterior acetabular fractures with a limited pararectus approach and the anatomical plates: preliminary results
Authors: Yao Sheng , Chen Kaifang , Zhu Fengzhao , Liu Jia , Wang Yulong , Zeng Lian , Wan Yizhou , Qu Yanzhen , Yang Liang , Yang Xu , Guo Xiaodong 
Published: December 2020

Abstract
Background The surgical treatment of acetabular fracture has adverse outcomes and high risk, and minimally invasive method is a good way to reduce complications and improve hip joint function. This study is to investigate the treatment of certain acetabular fractures primarily involving the anterior column and quadrilateral plate using a limited pararectus approach and the anatomical plates. Methods A consecutive cohort of 17 patients with anterior displaced acetabular fractures were managed operatively with a limited approach and the anatomical plates. Ten patients had anterior column fractures, 1 patient had anterior wall fracture, 4 patients had transverse fractures and 2 patients had anterior column with posterior hemi-transverse fractures. The inferior half of the pararectus approach was adopted to open the medial window and to access the anterior column and the quadrilateral plate. The anatomical plates were used for internal fixation. Residual displacements were assessed on the postoperative CT scans using a standardized digital method. The surgical details, hip functional outcomes, and complications were noted. Results All of the patients were operated using the limited pararectus approach and the anatomical plates successfully. The mean operative time and blood loss were 90.9 minutes and 334.1 ml, respectively. The average postoperative residual gap and step displacement on CT were 2.9 mm and 0.7 mm, respectively. The radiological outcome was estimated according to the Matta score, ten of the cases were graded anatomical, six were graded imperfect, and one was graded poor. Follow up averaged 15 months. Functional outcomes were excellent for nine, good for six, and fair for two. It was noted that one case of peritoneal injury was repaired intraoperatively.Conclusions The limited pararectus approach with the advantages of less trauma, direct exposure to the anterior column and quadrilateral plate. The anatomical plates can fit with the surface of the acetabulum, which saves the time of remodeling plates during operation and facilitate fracture reduction. The combination approach can be a good choice for limited surgery of displaced anterior acetabular fractures especially involving the quadrilateral plate.
Genome-wide identification of GH3 genes in Brassica oleracea and identification of a promoter region for anther-specific expression of a GH3 gene
Authors: Jeong Jiseong , Park Sunhee , Im Jeong Hui , Yi Hankuil 
Published: December 2020

Abstract
Background: The Gretchen Hagen 3 (GH3) genes encode acyl acid amido synthetases, many of which have been shown to modulate the amount of active plant hormones or their precursors. GH3 genes, especially Group Ⅲ subgroup 6 GH3 genes, and their expression patterns in economically important B. oleracea var. oleracea have not been systematically identified. Results: As a first step to understand regulation and molecular functions of Group Ⅲ subgroup 6 GH3 genes, 34 GH3 genes including four subgroup 6 genes were identified in B. oleracea var. oleracea. Synteny found around subgroup 6 GH3 genes in B. oleracea var. oleracea and Arabidopsis thaliana indicated that these genes are evolutionarily related. Although expression of four subgroup 6 GH3 genes in B. oleracea var. oleracea is not induced by auxin, gibberellic acid, or jasmonic acid, the genes show different organ-dependent expression patterns. Among subgroup 6 GH3 genes in B. oleracea var. oleracea, only BoGH3.13-1 is expressed in anthers when microspores, polarized microspores, and bicellular pollens are present, similar to two out of four syntenic A. thaliana subgroup 6 GH3 genes. Detailed analyses of promoter activities further showed that BoGH3.13-1 is expressed in tapetal cells and pollens in anther, and also expressed in leaf primordia and floral abscission zones. Conclusions: Sixty-two base pairs (bp) region (-340 ~ -279 bp upstream from start codon) and about 450 bp region (-1489 to -1017 bp) in BoGH3.13-1 promoter are important for expressions in anther and expressions in leaf primordia and floral abscission zones, respectively. The identified anther-specific promoter region can be used to develop male sterile transgenic Brassica plants.
Remoção cirúrgica de lipoma por acesso pré-auricular
Authors: Boscaine Evelyn de Freitas , Mendonça José Carlos Garcia de , Paiva-Oliveira Janayna Gomes , Pelissaro Gustavo Silva , Herculano Aline Bergman de Souza , Figueiredo Francielly Thomas , Castillo Daisilene Baena , Gaetti-Jardim Ellen Cristina 
Published: December 2020

Os lipomas são tumores benignos do tecido adiposo mais frequentes no tronco e nas extremidades do corpo, com incidência baixa na região orofacial. Clinicamente apresentam-se com lesão única ou lobular, assintomático, com crescimento lento, de base séssil, bem delimitados e macios, com coloração amarelada quando mais superficiais, a rósea em regiões mais profundas. O trabalho tem o objetivo de relatar um caso de lipoma pré-auricular em um paciente de sexo masculino, leucoderma encaminhado ao Serviço de Residência Bucomaxilofacial do Hospital Universitário da Universidade Federal de Mato Grosso do Sul queixando-se de dor na região. O tratamento foi cirúrgico sob anestesia geral, com acesso cirúrgico pré-auricular e exérese total da peça para análise histopatológica. O paciente segue em acompanhamento de 6 meses sem queixas ou alterações funcionais configurando até o momento o sucesso da terapêutica empregada.
Descritores: Lipoma; Neoplasias; Biópsia.
Referências

Costa DFN, Inaoka SD, Silverira KG, Souza NL, Santos LAM. Tratamento cirúrgico de lipoma: relato de dois casos. Rev cir traumatol buco-maxilo-fac. 2017;17(3):25-8.
Resende R, Meirelles M, Varella R. Remoção cirúrgica de lipoma de grande proporção: relato de caso. Rev cir traumatol buco-maxilo-fac. 2013;13(2):37-42.
Osterne RLV, Lima-Verde RMB, Turatti E, Cavalcante R. Lipoma de cavidade oral: um estudo de 101 casos em uma população brasileira. J Bras Patol Med Lab. 2019;55(2):148-59.
Tenório JR, Paiva KM, Nogueria PTB, Oliveira e Silva ED. Exérese de Extenso Lipoma em Região Submandibular: Relato de caso. Rev cir traumatol buco-maxilo-fac. 2013;13(3):37-40.
Menezes RO, Tavares SS, Peixoto TS, Aragão MS, Godoy GP. Unusual facial lipoma. RGO. 2014;62(4):425-30.
Mandarino SCA, Guimarães, MA, Coutinho MA. Lipoma em região parotídea: Relato de caso. Rev cir traumatol buco-maxilo-fac. 2014;14(3):33-8.
Shetty N, Shabari UB, Jaydeep NA, Patnaik P. Solitary lipoma in the retromandibular region. Indian J Dent. 2015;6(1):49-52.
Chuengue EK, Oliveira WD, Silva JPQ, Freitas DL. Remoção cirúrgica de lipoma volumoso em região temporal: Relato de caso. Rev Saberes. 2019;10(1).
Chia CY, Rovaris DA, Fontana R. Lipoma gigante do coxim adiposo bucal: relato de caso e revisão da literatura. Rev Bras Cir Plast. 2016;31(1):112-17.
de Souza Batista FR, Figueira JA, Lustosa RM, Eidt JM, Piacentini M, Pavan ÂJ. Lipoma in the Face Associated With Maxillofacial Trauma. J Craniofac Surg. 2017;28(1):295-96.
Mello DF, Manica MZ, Helene AJ. Lipomas gigantes: Séries de 14 casos. Rev Bras Cir Plast. 2015;30(1):33-7.
METASTATIC MULTIPLE PRIMARY GASTROINTESTINAL MELANOMA: A CASE REPORT
Authors: Kostromitsky D. N. , Dobrodeev A. Yu. , Afanasyev S. G. , Li N. A. , Vasilyev N. V. , Podyablonsky A. S. 
Published: December 2020

Primary gastrointestinal melanoma is a rare tumor with a frequency of 1 new case per 5–7 years. Over the past 100 years, there have been no more than 25 reported cases of primary melanoma of the stomach and colon. The rarity of the disease and nonspecific clinical features pose diagnostic challenges. The tumor is characterized by an aggressive growth and early metastasis. Case description. We present a rare clinical case of primary gastrointestinal melanoma with metastatic involvement of the adrenal glands, liver and retroperitoneal lymph nodes. Conclusion. To date, treatment standards for primary gastrointestinal melanoma have not been developed and are the subject of debate. However, palliative chemotherapy used for the treatment of primary skin melanoma may lead to disease stabilization in patients with gastrointestinal melanoma.
THE PRESENCE OF VARIOUS POPULATIONS OF CIRCULATING TUMOR CELLS IN THE BLOOD OF BREAST CANCER PATIENTS BEFORE TREATMENT: ASSOCIATION WITH FIVE-YEAR METASTASIS-FREE SURVIVAL
Authors: Kaigorodova E. V. , Tarabanovskaya N. A. , Surkova P. V. , Zelchan R. V. , Garbukov E. Yu. 
Published: December 2020

Localized and metastatic tumors are known to lead to the formation of circulating tumor cell (CTC ) clusters in the blood. Currently, there is a heightened interest in the study of molecular and biological characteristics of CTC s. Recent studies have shown the presence of different populations of CTC s in the blood of cancer patients. Some cells are cancer stem cells, some tumor cells undergo epithelial-mesenchymal transition (EMT), and most CTC s do not have features of either stem cells or EMT.The aim of the study was to evaluate the five-year metastasis-free survival rate in patients with invasive breast carcinoma, depending on the presence of various populations of circulating tumor cells in the blood before treatment.Material and Methods. A prospective study included 47 patients with newly diagnosed invasive breast cancer (T1–4N0–3M0), who were treated at Cancer Research Institute, Tomsk National Research Medical Center. The patients aged 31 to 69 years. The presence of different populations of CTC s in the blood of patients before treatment was determined by multicolor flow cytometry on the BD FACS Canto system, using different fluorochrome-labeled monoclonal antibodies to EpCam, CD 45, CD 44, CD 24, and N-cadherin. Five-year metastasis-free survival was evaluated by the Kaplan–Meier method. The differences were considered significant at p<0.05.Results. The results obtained demonstrated that the presence of both stem-like and non-stem CTC s showing signs of EMT with Epcam+CD 45-CD 44-CD 24-Ncadherin+, Epcam+CD 45-CD 44+CD 24-Ncadherin+, and Epcam(m)- CD 45-CD 44+CD 24-Ncadherin+ phenotypes in the blood of breast cancer patients before treatment reduced the five-year metastasis-free survival rate (p=0.0016, p=0.017 and p=0.011, respectively).Conclusion. Thus, CTC s in the EMT state are informative for liquid biopsy to assess the risk of hematogenous metastasis and can be considered as targets for selection of personalized chemotherapy.
ASSOCIATION OF NKT- AND ACTIVATED CD25<sup>+</sup> PERIPHERAL BLOOD LYMPHOCYTES WITH DISEASE FREE AND OVERALL SURVIVAL OF TRIPLE NEGATIVE BREAST CANCER PATIENTS
Authors: Chertkova A. I. , Slavina E. G. , Zabotina T. N. , Kadagidze Z. G. , Shoua E. K. , Gordeeva O. O. , Kolyadina I. V. , Zhukova L. G. , Gan’shina I. P. , Meshcheryakov A. A. 
Published: December 2020

Background. We previously found that a decrease in the number of NKT cells and activated CD 25+ peripheral blood lymphocytes (PBLs) before neoadjuvant chemotherapy was associated with an increased likelihood of disease progression in patients with locally advanced triple-negative breast cancer (TN BC).The purpose of this study was to determine the relationship between the initial number of NKT-and CD 25+ PBLs and relapsefree survival (RFS)/overall survival (OS ) in patients with TN BC who received neoadjuvant chemotherapy with cisplatin and paclitaxel followed by surgery.Material and Methods. The study included patients with stage II and III TN BC. The follow-up time was 36 and 66.9 months. Immediately before chemotherapy, the percentage of CD 3+CD 16+CD 56+ (NKT) -, CD 25+- and CD 8+ PBLs was determined by flow cytometry. Statistical analysis of the data was carried out using the Statistics 7 software package. The Kaplan-Meier method was used to determine the relationship between immunological parameters and RFS/ OS .Results. The decreased level of NKT cells before treatment was associated with a decrease in the 3-year RFS [Me: 20.1 (0.533 and 39.7) months] compared to that observed in patients with higher percentage of these cells than in the control (Me was not achieved). There were no statistically significant differences in the 3-year OS between the groups. The initially reduced number of CD 25+ lymphocytes in comparison with the control was associated with decreased rates of both RFS and OS . The difference in DFS and OS was more significant between the groups of patients who simultaneously had an increased initial number of both NKT and CD 25+ cells and patients in whom both cell populations were below normal levels.Conclusion. The initial (prior to chemotherapy) number of NKT and activated CD 25+ PBLs can apparently be a predictive factor in TN BC patients, who received neoadjuvant chemotherapy with cisplatin and paclitaxel.
METHODOLOGICAL ASPECTS OF PHOTODYNAMIC THERAPY OF EHRLICH SOLID CARCINOMA IN BALB/C MOUSE STRAIN WITH VARIOUS TUMOR LOCALIZATION
Authors: Kruglov S. S. , Gelfond M. L. , Tyndyk M. L. , Maydin M. A. , Grishacheva T. G. , Basina R. M. , Gubareva E. A. , Plakhov E. A. , Kireeva G. S. , Panchenko A. V. 
Published: December 2020

Background. Photodynamic Therapy is one of the treatment methods used in modern oncology. Evaluation of the efficacy in vivo of photosensitizers on tumor models is generally accepted, but the photodynamic therapy technique in mice is not without drawbacks.The purpose of the study was evaluation of the efficacy of photodynamic therapy in mice with Ehrlich tumor model after subcutaneous and intracutaneous injection of tumor cells.Material and Methods. The study was conducted on BAL B/C mice of both sexes. Fotoditazin® and Radachlorin® were used as photosensitizers. For photoactivation, the Alod laser apparatus with a wavelength of 662 nm was used.Results. A comparison of photodynamic therapy with subcutaneous and intracutaneous localization of Ehrlich tumor was performed. It was shown that depending on the location and depth of inoculation of Ehrlich tumor, the pharmacokinetics (both the fluorescence intensity over time and the contrast ratio of the tumor/surrounding tissue) and pharmacodynamics (tumor growth inhibition, survival) of photosensitizers are significantly different. Higher contrast of the tumor/surrounding tissue is observed with intracutaneous localization of the tumor.Conclusion. A model with intracutaneous localization of Ehrlich tumor can be recommended for a primary assessment of efficacy; it allows the use of fewer animals in the experiment. When planning experiments to study photosensitizers and evaluating their results, the advantages and disadvantages of different methods for modeling tumors in mice should be taken into account.
EFFECT OF THE MYCELIUM EXTRACT FROM DUDDINGTONIA FLAGRANS FUNGUS ON SUBCUTANEOUS XENOGRAFT OF HUMAN C33a CERVICAL CARCINOMA CELLS
Authors: Solovieva O. I. , Zavjalov E. L. , Teplyakova T. V. , Lebedev L. R. , Razumov I A. 
Published: December 2020

The purpose of the study was to analyze the antitumor effects of the extract of mycelium from Duddingtonia flagrans (strain F-882) on xenografts of human C33a cervical cancer cells.Material and Methods. To evaluate the antitumor effect, we used the absolute values of xenograft volumes and calculated the tumor growth inhibition and the index of tumor growth.Results. At the first stage of the experiment, a 4-week subcutaneous injection of the water extract of F-882 resulted in an almost twofold slowdown in xenograft growth, with the tumor growth inhibition value of 50.6 %. In the second stage of the experiment, a 2.5-week subcutaneous injection followed by a 1.5-week intratumoral injection of F-882 also caused the tumor growth inhibition. After completing F-882 injections, the effect of tumor growth inhibition continued for 2.5 weeks and the tumor growth inhibition value was 58.7 %.Conclusion. The mycelium extract F-882 was shown to have an antitumor effect on subcutaneous xenografts of human C33a cervical carcinoma cells.