bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness Study of a Cultural Adaptation of Cognitive‐Behavioural Therapy‐Based Application for Depressive Symptoms in College Students: A Randomised Controlled Trial
Authors: Lin Yueh‐Hsiu , Wu Chia‐Yi , Gau Bih‐Shya , Lin Cheng‐Han , Ho Hsiu‐Yu , Lou Meei‐Fang 
Published: December 2024

ABSTRACTIntroductionStudies on mental health applications among college students lack empirical evidence regarding mobile application usage's intensity, duration and frequency.AimTo evaluate the effectiveness of a cognitive‐behavioural therapy (CBT)‐based mobile application in ameliorating depressive symptoms among college students.MethodsA randomised controlled trial was conducted at three universities. Undergraduates aged ≥ 20 years with self‐reported depressive symptoms were recruited and randomly assigned to the control and intervention groups. The outcomes include changes in depressive symptoms, suicidal ideation, help‐seeking attitudes and emotional self‐awareness at 4 (T1), 8 (T2) and 12 (T3) weeks. An intention‐to‐treat analysis was conducted.ResultsOf the 91 participants, 46 in the intervention group exhibited a significant decline in depressive symptoms at T1 and lower patterns of symptom changes at T2 and T3. No significant differences were observed in the other outcomes; however, higher help‐seeking attitude, emotional self‐awareness and lower suicidal ideation after T0 were observed for those who used the application for 12 weeks.DiscussionThe advantages of a brief application‐based intervention to promote college students' psychological health are outlined.ImplicationsSchool nursing managers could use the application to improve the college mental health systems and support collegial teacher–student relationships.RecommendationsFurther studies should enhance application usage to manage depressive symptoms.Trial RegistrationThe study was approved by the Research Ethics Committee of the National Taiwan University Hospital (No. 202108098RINB, Date of approval: Oct 27, 2021) and the National Institutes of Health (No. NCT05188950).
Are alarm symptoms of the Rome IV criteria enough to rule out organic pathology?
Authors: Gürcan Kaya Neslihan , Öztürk Hakan 
Published: December 2024

AbstractBackgroundThe Rome IV criteria offer symptom‐based recommendations for diagnosing functional gastrointestinal diseases (FGID) in children and adolescents without esophagogastroduodenoscopy (EGD). The aim of this study is to evaluate the usefulness of Rome IV criteria for ruling out organic disease in patients with and without alarm symptoms.MethodsRetrospective research was conducted on children and adolescents who had dyspepsia and underwent EGD. The patients were categorized into groups according to the Rome IV criteria, based on the presence or absence of alarm signs. The esophagogastroduodenoscopy findings in these groups were then compared and classified as either altered or normal.ResultsSeventy‐seven of 124 cases (62%) had altered EGD findings, and 97.2% (75/77) of altered EGD patients had histopathological findings. In the study population, the prevalence of Helicobacter pylori was 46.7%. Seventy‐four of 124 cases (59.7%) had no alarm symptoms and were diagnosed with FGID according to Rome IV criteria. Thirty‐six (48%) of these patients had altered EGD, and five of these patients had duodenal ulcers. Patients without alarm symptoms were compared based on altered versus normal EGD findings. Helicobacter pylori was significantly more abundant in the altered EGD group. Fifty (40.3%) of 124 patients had one or more alarm symptoms and 41 of 50 (82%) cases with alarm symptoms had altered EGD. When patients with altered and normal EGD findings were compared, alarm symptoms, H. pylori, and histopathological findings were higher in the altered EGD group.ConclusionThe Rome IV criteria are effective for alarm‐symptom‐positive patients. However, in patients lacking alarm symptoms, the Rome IV criteria are insufficient to rule out organic causes.
Early eosinophilia after hematopoietic stem cell transplantation is associated with chronic graft‐versus‐host disease
Authors: Uemura Suguru , Kishimoto Kenji , Fujikawa Tomoko , Nakamura Sayaka , Kozaki Aiko , Saito Atsuro , Ishida Toshiaki , Mori Takeshi , Hasegawa Daiichiro , Kosaka Yoshiyuki 
Published: December 2024

AbstractBackgroundThis study aimed to evaluate whether an early elevation of absolute eosinophil count after allogeneic hematopoietic stem cell transplantation (allo‐HSCT) was associated with the development of chronic graft‐versus‐host disease (cGVHD) in children and adolescents.MethodsA total of 165 consecutive patients who received allo‐HSCT were included in the study. Patients who had previously received allo‐HSCT, relapsed or died before Day 100, and did not achieve engraftment, were excluded. Eosinophilia was defined as an eosinophil count exceeding 500/μL in peripheral blood within 60 days of stem cell infusion.ResultsEosinophilia was observed in 67 patients (40.6%). There were no significant differences in disease type, graft source, donor type, conditioning regimen, and GVHD prophylaxis and treatment between the patients with or without eosinophilia. The 3‐year cumulative incidence rate of cGVHD in the entire cohort was 39.3% (95% confidence interval (CI): 32.2–47.4). Patients with eosinophilia had a higher 3‐year cumulative incidence rate of cGVHD compared to those without eosinophilia (49.1% vs. 32.7%, p = 0.007). Cox proportional hazards model analysis revealed that eosinophilia was associated with an increased risk of cGVHD (adjusted hazard ratio: 2.12; 95% CI: 1.16–3.85, p = 0.014).ConclusionsEarly eosinophilia after allo‐HSCT may serve as a predictor for the development of cGVHD in children and adolescents.
Metabolic‐Engineering Approach to Enhance Vanillin and Phenolic Compounds in Ocimum Sanctum (CIM‐Angana) via VpVAN Overexpression
Authors: Husain Zakir , Khan Sana , Sarfraz Aqib , Iqbal Zafar , Chandran Ashish , Khatoon Kahkashan , Parween Gazala , Deeba Farah , Afroz Shama , Khan Feroz , Ch Ratnasekhar , Rahman Laiq ur 
Published: December 2024

AbstractTransgenic Ocimum sanctum plants were engineered to produce vanillin by overexpressing the VpVAN gene using Agrobacterium‐mediated transformation. Positive transformants developed shoots within 4–5 weeks and were transferred to a root induction medium and four independent transformants with no observed adverse effects were kept for anlysis. Quantitative RT‐PCR indicated significantly higher VpVAN expression in transgenic lines AG_3 and AG_1, impacting the phenylpropanoid pathway and phenolic compound accumulation. Molecular docking studies indicated ferulic acid's higher binding affinity to vanillin synthase than eugenol. LC–MS/MS analysis revealed a marked increase in vanillin production in transgenic lines compared to wild type, with AG_3 exhibiting the highest vanillin content (1.98 ± 0.0047 mg/g extract) and AG_1 following (1.49 ± 0.0047 mg/g extract). AG_3 also showed elevated levels of benzoic acid, 4‐hydroxy benzyl alcohol, and ferulic acid. This study highlights the potential of metabolic engineering in O. sanctum for enhanced vanillin production, suggesting pathways for large‐scale production of natural vanillin and other valuable compounds in transgenic plants.
Modern Plant Breeding for Achieving Global Food Security
Authors: Fiaz Sajid , Uzair Muhammad , Zhang Baohong 
Published: December 2024

AbstractModern plant breeding technologies have played a central role in addressing global food security challenges. These technologies, including next‐generation sequencing (NGS) and multi‐omics analysis, genome‐wide association analysis (GWAS), genome editing and transgenics, machine learning, and speed breeding, have been improving crop yield and quality as well as crop adaptability under climate change conditions, such as tolerance to both biotic and abiotic stresses. Furthermore, identification, searching, assessment, and combining desirable integrated (morphological, physiological, and biochemical) attributes have been achieved with greater accuracy, efficiency, time, and cost‐effectiveness, all of which are essential to meeting global food demands. These advancements hold promises for increasing food security in the face of population growth and climate change, ensuring a more resilient and sustainable food production system. Therefore, this topical collection was developed to feature the latest developments in modern plant breeding approaches to understand and improve plant attributes essential for ensuring sustainable crop production.
Autonomous Freight Takes to the Rail, Road, Sea, and Air
Authors: Tom Gibson 
Published: December 2024

Abstract
Driverless cars are already on the road. Now, a parallel trend is now on the rise, bringing autonomy to freight transportation. Engineers are launching companies that utilize driverless trucks, trains, boats, and drones to ship and deliver goods. While these modes of autonomous transit may not be as provocative as an air taxi, they are already improving supply chains.
Soil microbiome interventions for carbon sequestration and climate mitigation
Authors: Beattie Gwyn A. , Edlund Anna , Esiobu Nwadiuto , Gilbert Jack , Nicolaisen Mette Haubjerg , Jansson Janet K. , Jensen Paul , Keiluweit Marco , Lennon Jay T. , Martiny Jennifer , Minnis Vanessa R. , Newman Dianne , Peixoto Raquel , Schadt Christopher , van der Meer Jan Roelof 
Published: December 2024

ABSTRACT

Mitigating climate change in soil ecosystems involves complex plant and microbial processes regulating carbon pools and flows. Here, we advocate for the use of soil microbiome interventions to help increase soil carbon stocks and curb greenhouse gas emissions from managed soils. Direct interventions include the introduction of microbial strains, consortia, phage, and soil transplants, whereas indirect interventions include managing soil conditions or additives to modulate community composition or its activities. Approaches to increase soil carbon stocks using microbially catalyzed processes include increasing carbon inputs from plants, promoting soil organic matter (SOM) formation, and reducing SOM turnover and production of diverse greenhouse gases. Marginal or degraded soils may provide the greatest opportunities for enhancing global soil carbon stocks. Among the many knowledge gaps in this field, crucial gaps include the processes influencing the transformation of plant-derived soil carbon inputs into SOM and the identity of the microbes and microbial activities impacting this transformation. As a critical step forward, we encourage broadening the current widespread screening of potentially beneficial soil microorganisms to encompass functions relevant to stimulating soil carbon stocks. Moreover, in developing these interventions, we must consider the potential ecological ramifications and uncertainties, such as incurred by the widespread introduction of homogenous inoculants and consortia, and the need for site-specificity given the extreme variation among soil habitats. Incentivization and implementation at large spatial scales could effectively harness increases in soil carbon stocks, helping to mitigate the impacts of climate change.
Effect of Endophytic Bacteria Bacillus subtilis 26D and Bacillus velezensis M66 on Resistance of Potato Plants to the Causative Agent of Early Blight Alternaria solani
Authors: Sorokan A. V. , Gabdrakhmanova V. F. , Mardanshin I. S. , Maksimov I. V. 
Published: December 2024

Abstract
The effect of Bacillus velezensis M66 and Bacillus subtilis 26D bacteria on the resistance of potato plants to the necrotrophic fungus Alternaria solani (the causative agent of early blight) was studied. The accumulation of viable bacterial cells of these strains in the internal tissues of the potato stem, roots, and tubers over a long period of time was demonstrated for the first time. A significant reduction in the area affected by the early blight on leaves inoculated with plant endophytes, as well as inhibition of the pathogen growth under the action of bacterial strains, was detected, which can be explained by the synthesis of lipopeptide antibiotics (the genes responsible for the synthesis of which were found by the PCR method) and proteolytic enzymes (the activity of which was demonstrated in vitro). The formation of plant resistance under the influence of inoculation with B. subtilis 26D and B. velezensis M66 was accompanied by an accumulation of hydrogen peroxide in the first hours after plant infection with A. solani spores and by a decrease in this index at the late stages of pathogenesis due to an increase in the activity of catalase and peroxidases. Limitation of the fungus spread was accompanied by an increase in the activity of proteinase inhibitors in plants, which probably decreased the negative effect of proteolytic enzymes of the necrotrophic pathogen A. solani. It can be assumed that the inoculation of plants with bacterial cells of the B. velezensis M66 strain contributed to the formation of resistance of potato plants to the early blight by efficient priming of the phytoimmune potential, which is comparable to the B. subtilis 26D strain (successfully used in the field conditions), an active component of the Fitosporin-M biopreparation.
Peroxidase of Trametes hirsuta LE-BIN 072: Purification, Characteristics, and Application for Dye Decolorization
Authors: Savinova O. S. , Fedorova T. V. 
Published: December 2024

Abstract
Lignin peroxidase (LiP9) from the basidiomycete Trametes hirsuta LE-BIN 072, an effective lignin destructor, was purified to a homogeneous state (with an RZ purity index of 1.8) and characterized for the first time. The molecular weight of LiP9 was 43 kDa and its pI was 3.2. The enzyme showed the highest activity at pH 2.5 and 35°C when veratryl alcohol was used as a substrate. The analysis of the substrate specificity showed that LiP9 oxidized phenol derivatives much faster than those of benzoic and cinnamic acids with the same substituents in the benzene ring. The highest specific activity of the enzyme was observed for catechol oxidation. The ability of LiP9 to decolorize recalcitrant dyes (reactive black 5, congo red, remazol brilliant blue R, phenol red, indigo carmine, and bromocresol green) was assessed. The highest decolorization efficiency was shown for indigo carmine (in the presence of veratryl alcohol) and bromocresol green (directly) up to 80 and 60%, respectively, in 1 hour.
Sustainable Distributed Scheduling Problem
Authors: Saraeian Shideh 
Published: December 2024

Many strategies have been devised to facilitate scheduling determinations per the dynamic production landscape. Also, the repercussions of environmental degradation intensify, and the focus on sustainable production methodologies has acquired substantial recognition. In addition, production managers are required to carefully determine the suitable schedule for each machine, considering environmental criteria. In such cases, another challenge in the manufacturing system alongside the minimization of job completion time which is important is reducing the environmental parameters impacts. Hence, this study focuses on addressing the challenge of distributed scheduling problems within the context of sustainable production. For energy-efficient factory selection, in this research, support vector machine (SVM) algorithm and improved particle swarm optimization (IPSO) have been used. In this case, first, factories with the least amount of pollution, waste, and energy consumption were selected. Second, the composition of intelligent algorithms such as gravitational search algorithm (GSA) and genetic algorithm (GA) was used to propose an appropriate distributed jobs scheduling in selected distributed factories. The simulation results show that this intelligent scheduling of distributed sustainable factories has significant potential to minimize environmental parameters inside production cycle optimization.
Analysis of Fibroblast Growth Factor 2 Impact and Mechanism on Broncho-Pulmonary Dysplasia
Authors: Lu Ling , Liu Ye-Dan , Liu Dong-Yun , Jin Rong , Qu Zheng-Hai 
Published: December 2024

Objective:Epithelial-mesenchymal transition (EMT) of alveolar epithelial cells is an important mechanism for the onset and development of broncho-pulmonary dysplasia (BPD).The role of FGF-2 in BPD is currently unclear. The aim of our study is to investigate the expression of FGF-2 in lung tissue of BPD mice, to further clarify the effect of FGF-2 on EMT in alveolar epithelial cells and to actively search for possible signaling pathways. Methods: The BPD model was induced by exposure to hyperoxia. Lung tissue samples were collected and Hematoxylin and eosin (HE) staining was used to determine the modelling effect. Quantitative Real-time Polymerase Chain Reaction (QRT-PCR), immunohistochemistry were used to detect FGF-2 expression in BPD mice. To further investigate the effect of FGF-2 supplementation and deficiency on EMT in alveolar epithelial cells, A549 cells were cryopreserved, resuspended, cultured and passaged. Transforming growth factor-β1 (TGFβ1) was used to induce EMT. FGF-2 small interfering RNA fragments were synthesised and screened. Fbroblast growth factor receptor1 (FGFR1) expression was inhibited by BGJ398. (3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium) (MTS) assay was used to detect the effect of FGF-2 and Infigratinib (BGJ398) on cell proliferation. We used qRT-PCR and Western blot to detect the expression of epithelial cell markers, mesenchymal cell markers and EMT-related signaling pathway proteins. Results: Our results showed that the successful established hyperoxia mice model were characteristic by BPD. Hyperoxia decreased FGF-2 on day 4, upregulated FGF-2 on day 21, which resulted in EMT. In vitro, we found that FGF-2 alone increased the expression of mesenchymal markers, decreased the expression of epithelial markers and activated Phosphatidylinositol 3-kinase/Protein kinase B (PI3K/AKT), Small mother against decapentaplegic (Smad), mitogen-activated protein kinase (P38) and extracellular signal-regulated kinase (ERK) signaling pathways. FGF-2 could not reverse but synergistically promote TGF-β1-induced EMT of alveolar epithelial cells. Silencing FGF-2 increased the expression of epithelial marker E-cadherin, inhibited the PI3K/AKT, Smad, and P38 signaling pathways activated by TGF-β1, but activated ERK signaling. FGF-2 receptor inhibitor BGJ398 reversed TGF-β1-induced EMT, decreased the expression of FGFR1, and inhibited ERK signaling pathway activation. Conclusions: FGF2 was closely associated with EMT in BPD mice. Both high and low levels of FGF2 promoted EMT in A549. The FGF-2 receptor inhibitor BGJ398 reversed TGF-β1-induced EMT in A549 by inhibiting the FGFR1/P-ERK signaling pathway.
Profilaxia pós-exposição ocupacional contra o vírus da imunodeficiência humana para trabalhadores da área de saúde vítimas de acidentes com material biológico
Authors: Frison Fernanda Sucasas , Alonzo Herling Gregório Aguilar , Pavan Maria Helena , Guerreiro Inajara de Cássia 
Published: December 2024

Introduction: Postexposure prophylaxis (PEP) reduces the risk of human immunodeficiencyvirus (HIV) transmission after workplace exposure of health care workers. Objectives: Toevaluate adherence to postexposure prophylaxis for HIV, incident characteristics, post-incidentinitiation of therapy, and reported adverse effects among treated health care workers. Methods: Epidemiologic, descriptive, retrospective study based on analysis of workplace incident reports involving exposure to biological materials from 2011 to 2020 at a university hospital. Data were analyzed using descriptive statistics, odds ratio using simple logistic regression, and comparison of variables using Fisher’s exact test (p<0.05). Results: A total of 516 cases eligible for prophylaxis were analyzed and divided into three risk groups: 168 (32.6%) involving HIV-positive source patients, 263 (50.9%) involving source patients with unknown infection status, and 85 (16.4%) involving source patients in the window period. Most cases involved females (74.80%), injuries from percutaneous exposure (80.42%), contact with blood (56.59%), and nursing technicians (30.46%). Full compliance and adherence with drug therapy (28 days) was reported in 77.45% of the workers, and the time between the incident and the start of prophylaxis was within 24 hours of the incident in 94.27% of the workers. In total, 205 (39.73%) of the workers discontinued outpatient follow-up, which was associated with education, risk group, and occupation. Conclusions: Incidents involving contaminated sharp instruments pose an increased risk of infection by pathogens transmitted primarily by blood. Workplace HIV infection can be prevented by providing mandatory training programs for workers on proper work practices, use of devices with retractable needles and needle protection systems, safe and proper disposal of materials,use of personal protective equipment, and appropriate workplace postexposure interventions, including PEP. None of the workers who completed outpatient follow-up seroconverted to HIV, thus demonstrating the protective effect of PEP. Regular training is recommended, with an emphasis on incident prevention, infection control, and health education, combined with case reporting strategies.
Finite to Non-finite through Impersonalization
Authors: Kozhanov Kirill 
Published: December 2024

Abstract
This study examines the contact-induced emergence of an infinitive in Russian Romani, a northeastern Romani dialect spoken in Russia and neighboring countries. Romani, like other Balkan languages, lacks an infinitival verbal form and instead uses finite subjunctive phrases in complements and purpose clauses. However, in some Romani dialects that have been in contact with infinitival languages (e.g., Slavic, Germanic, Finnish), a new infinitive form has emerged. This new form, derived from the subjunctive, lacks agreement with the controller. Drawing on spoken and literary corpus data, this article explores the development of several new non-agreeing verbal forms from generic impersonal uses. Unlike other Romani dialects, in Russian Romani, this new form has not fully grammaticalized into an infinitive but mainly functions as a default in generic and agent-demoted contexts.
Sacred Spaces
Authors: Wibowo Waskito 
Published: December 2024

Abstract
It is familiarly known among Muslims that Sharīʿa suggests the prohibition of participating in church construction. However, in historical accounts, a number of churches in Arab countries were built during the era of Islam. This led to ambiguities and the perception that the caliphs did not abide by Sharīʿa law then. Therefore, this essay aims to reveal the reasons and motives of those caliphs or other Muslim authority holders allowing the building of churches in Cairo during the Mamluk dynasty. Furthermore, this article seeks the extent to which Muslim authority holders contribute to the church building for Christians and the responses, attitudes and dynamics of Muslim residents around the churches built in their area. By employing the qualitative library method, by making primary and secondary pieces of literature as objects of study and then interpreting them from a historical point of view, this article indicates that life under the Mamluk dynasty was so dynamic. There was a different application for the restriction of building, rebuilding, or reopening churches between society and some sultans. While citizens acted massive attacks and destruction of the churches provoked by the fatwā of the elite, it seems there were also defenses from the authorities for this act of vandalism.