bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal ω3 docosapentaenoic acid inhibits infant allergic dermatitis through TRAIL‐expressing plasmacytoid dendritic cells in mice
Authors: Hirata So‐ichiro , Nagatake Takahiro , Sawane Kento , Hosomi Koji , Honda Tetsuya , Ono Sachiko , Shibuya Noriko , Saito Emiko , Adachi Jun , Abe Yuichi , Isoyama Junko , Suzuki Hidehiko , Matsunaga Ayu , Tomonaga Takeshi , Kiyono Hiroshi , Kabashima Kenji , Arita Makoto , Kunisawa Jun 
Published: February 2020

AbstractBackgroundMaternal dietary exposures are considered to influence the development of infant allergies through changes in the composition of breast milk. Cohort studies have shown that ω3 polyunsaturated fatty acids (PUFAs) in breast milk may have a beneficial effect on the preventing of allergies in infants; however, the underlying mechanisms remain to be investigated. We investigated how the maternal intake of dietary ω3 PUFAs affects fatty acid profiles in the breast milk and their pups and reduced the incidence of allergic diseases in the pups.MethodsContact hypersensitivity (CHS) induced by 2,4‐dinitrofluorobenzene (DNFB) and fluorescein isothiocyanate was applied to the skin in pups reared by mother maintained with diets mainly containing ω3 or ω6 PUFAs. Skin inflammation, immune cell populations, and expression levels of immunomodulatory molecules in pups and/or human cell line were investigated by using flow cytometric, immunohistologic, and quantitative RT‐PCR analyses. ω3 PUFA metabolites in breast milk and infant's serum were evaluated by lipidomics analysis using LC‐MS/MS.ResultsWe show that maternal intake of linseed oil, containing abundant ω3 α‐linolenic acid, resulted in the increased levels of ω3 docosapentaenoic acid (DPA) and its 14‐lipoxygenation products in the breast milk of mouse dams; these metabolites increased the expression of TNF‐related apoptosis‐inducing ligand (TRAIL) on plasmacytoid dendritic cells (pDCs) in their pups and thus inhibited infant CHS. Indeed, the administration of DPA‐derived 14‐lipoxygenation products to mouse pups ameliorated their DNFB CHS.ConclusionThese findings suggest that an inhibitory mechanism in infant skin allergy is induced through maternal metabolism of dietary ω3 PUFAs in mice.
The role of testosterone in menopausal hormone treatment. What is the evidence?
Authors: Johansen Nora , Lindén Hirschberg Angelica , Moen Mette H. 
Published: February 2020

AbstractAbout 40% of postmenopausal women have decreased sexual desire, causing distress. Estrogen therapy attenuates vaginal complaints but has no effect on sexual desire. Although sexual function has been linked to testosterone, there is no clear relation between sexual desire and circulating levels of testosterone. Nevertheless, treatment with transdermal (patch) testosterone improved sexual function in several randomized controlled trials. Women with hypoactive sexual desire disorder who were treated with testosterone reported more satisfying sexual episodes and sexual desire compared with the placebo group. Adverse effects were mild. However, there is no testosterone drug designed for women available on the European market. Consequently, women who opt for testosterone treatment have to use preparations made for men with a high drug concentration. Adequate dosage for women is therefore challenging. A trial of 5 mg transdermal testosterone (gel or cream) daily or less has been suggested, followed by close monitoring of side effects and hormone level.
Strategies discussed at the XIIth international conference on Kangaroo mother care for implementation on a countrywide scale
Authors: Charpak Nathalie , Angel María I. , Banker Deepa , Bergh Anne‐Marie , María Bertolotto Ana , De Leon‐Mendoza Socorro , Godoy Natalia , Lincetto Ornella , Lozano Juan M. , Ludington‐Hoe Susan , Mazia Goldy , Mokhachane Mantoa , Montealegre Adriana , Ramirez Erika , Sirivansanti Nicole , Solano Jose Maria , Day Louise-Tina , Uy Maria Esterlita 
Published: February 2020

AbstractAimBuilding strategies for the country‐level dissemination of Kangaroo mother care (KMC) to reduce the mortality rate in preterm and low birth weight babies and improve quality of life. KMC is an evidence‐based healthcare method for these infants. However, KMC implementation at the global level remains low.MethodsThe international network in Kangaroo mother brought 172 KMC professionals from 33 countries together for a 2‐day workshop held in conjunction with the XIIth International KMC Conference in Bogota, Colombia, in November 2018. Participants worked in clusters to formulate strategies for country‐level dissemination and scale‐up according to seven pre‐established objectives.ResultsThe minimum set of indicators for KMC scale‐up proposed by the internationally diverse groups is presented. The strategies for KMC integration and implementation at the country level, as well as the approaches for convincing healthcare providers of the safety of KMC transportation, are also described. Finally, the main aspects concerning KMC follow‐up and KMC for term infants are presented.ConclusionIn this collaborative meeting, participants from low‐, middle‐ and high‐income countries combined their knowledge and experience to identify the best strategies to implement KMC at a countrywide scale.
Comparison of MALDI‐TOF mass spectrometry analysis of peripheral blood and bone marrow‐based flow cytometry for tracking measurable residual disease in patients with multiple myeloma
Authors: Eveillard Marion , Rustad Even , Roshal Mikhail , Zhang Yanming , Ciardiello Amanda , Korde Neha , Hultcrantz Malin , Lu Sydney , Shah Urvi , Hassoun Hani , Smith Eric , Lesokhin Alexander , Mailankody Sham , Landgren Ola , Thoren Katie 
Published: February 2020

SummaryMatrix‐assisted laser desorption ionisation time‐of‐flight mass spectrometry (MALDI‐TOF MS) may soon replace routine electrophoretic methods for monitoring monoclonal proteins in patients with multiple myeloma. To further evaluate the clinical utility of this assay, we compared the performance of MALDI‐TOF‐MS head‐to‐head with an established bone marrow‐based measurable residual disease assay by flow cytometry (Flow‐BM‐MRD), using Memorial Sloan Kettering Cancer Center’s 10‐color, single‐tube method. Our results suggest that MALDI‐TOF‐MS adds value to bone marrow‐based MRD testing and may be most useful for early detection of relapse in peripheral blood compared to current electrophoretic methods.
Geological mapping reveals the role of Early Jurassic rift architecture in the dispersal of calciturbidites: New insights from the Central and Northern Apennines
Authors: Cipriani Angelo , Caratelli Martina , Santantonio Massimo 
Published: February 2020

AbstractThe role of sea‐bottom topography in the dispersal of shallow water‐derived calciturbidites across a submarine rift, as determined by the local extensional architecture, is under‐investigated, namely with pelagic settings along ancient passive continental margins. A comparison with modern carbonate platform/basin analogues, or with siliciclastic systems, is not always feasible, as ancient carbonate systems were commonly home to anachronistic environments (e.g. the Western Tethyan Mesozoic). Our study focuses on: (a) a reconstruction of the palaeotectonic architecture of the Umbria‐Marche Basin in the Jurassic and on (b) how this architecture produced a submarine topography which governed the dispersal of sediment, shed by the neighbouring Lazio‐Abruzzo Carbonate Platform, for >40 million years. A geological mapping project was performed in the Apennines of Central Italy, a region which in the Jurassic displayed a pattern of intrabasinal highs (pelagic carbonate platforms) and intervening basins which was exceedingly complex due to the high density, and oddly variable trends, of faults rooted in a shallow detachment layer corresponding to thick Triassic salt. A map pairing the occurrences of these resedimented beds with an updated palaeogeography becomes the natural descriptor of the itineraries followed by sediment gravity flows. This qualitative method represents a companion, or even alternative, approach to the one strictly based on physical stratigraphy, and it greatly improves our knowledge of regional geology and rift‐basin analysis. Our case history potentially represents the analogue of hydrocarbon fields both inland and in the offshore. Geological mapping shows that the marginal palaeoescarpments of pelagic carbonate platforms formed obstacles to the gravity flows as sediment load was discharged at their toes. While turbidity flows were locally vigorous enough to climb the escarpments, leaving overbank deposits on the pelagic carbonate platform‐tops, a ‘shelter’ effect is evidenced by the resediment‐free nature of those basins lying downflow, which were shielded by the highs.
Cellular calcium and redox regulation: the mediator of vertebrate environmental sex determination?
Authors: Castelli Meghan A. , Whiteley Sarah L. , Georges Arthur , Holleley Clare E. 
Published: February 2020

ABSTRACTMany reptiles and some fish determine offspring sex by environmental cues such as incubation temperature. The mechanism by which environmental signals are captured and transduced into specific sexual phenotypes has remained unexplained for over 50 years. Indeed, environmental sex determination (ESD) has been viewed as an intractable problem because sex determination is influenced by a myriad of genes that may be subject to environmental influence. Recent demonstrations of ancient, conserved epigenetic processes in the regulatory response to environmental cues suggest that the mechanisms of ESD have a previously unsuspected level of commonality, but the proximal sensor of temperature that ultimately gives rise to one sexual phenotype or the other remains unidentified. Here, we propose that in ESD species, environmental cues are sensed by the cell through highly conserved ancestral elements of calcium and redox (CaRe) status, then transduced to activate ubiquitous signal transduction pathways, or influence epigenetic processes, ultimately to drive the differential expression of sex genes. The early evolutionary origins of CaRe regulation, and its essential role in eukaryotic cell function, gives CaRe a propensity to be independently recruited for diverse roles as a ‘cellular sensor’ of environmental conditions. Our synthesis provides the first cohesive mechanistic model connecting environmental signals and sex determination pathways in vertebrates, providing direction and a framework for developing targeted experimentation.
Effects of affordances in the home environment on children's personal‐social, problem‐solving, and communication skills
Authors: Valadi Saeed , Gabbard Carl , Hooshyari Faramarz 
Published: February 2020

AbstractBackgroundAmong the various factors forming the environment, most developmentalists are in agreement that the home is the primary agent for change in child growth and development. The present study investigated the effect of affordances in the home on personal‐social, problem‐solving, and communication skills of children between 18 and 42 months of age. This investigation extends work focusing on “motor” affordances as an environmental feature.MethodsUsing cross‐sectional design, 251 families (124 boys and 127 girls) were evaluated using the Persian translation of Affordances in the Home Environment for Motor Development and the Ages and Stages Inventories to determine the three skill competencies.ResultsResults showed that the level of home affordances significantly impacted all three skill areas. And the total variance of predictive values in the final model had a moderate and significant ratio. Other factors such as attendance and non‐attendance of children in kindergarten, family income, and education of parents were also contributing factors.ConclusionsIn general, these findings indicate that the home environment plays an important role in the development of personal‐social, problem‐solving, and communication skills.
IgG antibody response against Plasmodium falciparum aminopeptidase 1 antigen in Gabonese children living in Makokou and Franceville
Authors: Oyegue-Liabagui S L , Imboumy-Limoukou R-K , Kouna C L , Bangueboussa F , Schmitt M , Florent I , Lekana-Douki J B 
Published: February 2020

Summary
The search for novel chemical classes of anti-malarial compounds to cope with the current state of chemoresistance of malaria parasites has led to the identification of Plasmodium falciparum aminopeptidase 1 (PfA-M1) as a new therapeutic target. PfA-M1, known to be involved in the hemoglobin digestion cascade which helps to provide most of the amino acids necessary to the parasite's metabolism, is currently considered as a promising target for anti-malarial chemotherapy. However, its immunogenic properties have not yet been tested in the Gabonese population. In Gabon, the prevalence of malaria remains three times higher in semi-urban areas (60·12%) than in urban areas (17·06%). We show that malaria-specific PfA-M1 antibodies are present in children and increase with the level of infection. Children living in semi-urban areas have higher anti-PfA-M1 antibody titers (0·14 ± 0·02 AU) than those living in urban areas (0·08 ± 0·02 AU, P = 0·03), and their antibody titers increase with age (P &lt; 0·0001). Moreover, anti-PfA-M1 antibody titers decrease in children with hyperparasitemia (0·027 ± 0·055 AU) but they remain high in children with low parasite density (0·21 ± 0·034 AU, P= 0·034). In conclusion, our results suggest that malaria-specific PfA-M1 antibodies may play an important role in the immune response of the host against P. falciparum in Gabonese children. Further studies on the role of PfA-M1 during anemia are needed.
A 3‐year longitudinal prospective study assessing microbial profile and clinical outcomes of single‐unit cement‐retained implant restorations: Zirconia versus titanium abutments
Authors: de Oliveira Silva Thalisson S. , de Freitas Alice R. , de Albuquerque Rubens F. , Pedrazzi Vinícius , Ribeiro Ricardo F. , do Nascimento Cássio 
Published: February 2020

AbstractPurposeThe aim of this study was to assess the microbiological and clinical outcomes of implant‐supported restorations on zirconia or titanium abutments after 3 years in function.Materials and methodsTwenty two‐part dental implants were placed in 20 healthy individuals in need of single‐tooth replacement. Ceramic‐based reconstructions were cemented in either zirconia or titanium abutments. Clinical, radiographic, and microbiological outcomes were examined at implant loading and then yearly up to 3 years post‐loading.ResultsCumulative survival/success implant rates were 95% after 3 years. Mean total marginal bone loss was 0.76 ± 0.21 mm for zirconia and 0.99 ± 0.41 mm for titanium, with no significant differences (P > .05). Overall, titanium and zirconia abutments presented similar values of probing depth, gingival recession, and bleeding on probing over time (P < .05). Microbial profile of implants restored with titanium or zirconia is quite similar to that found in the remaining teeth.ConclusionsZirconia and titanium presented different microbial profile and genome counts. Clinical findings for both zirconia and titanium abutments were similar and consistent with a healthy condition, reflecting a high survival rate and low bone loss. Microbiota did not impact the clinical outcomes after 3 years of function.
Prognostic factors influencing survival in patients undergoing cytoreductive surgery with hyperthermic intraperitoneal chemotherapy for isolated colorectal peritoneal metastases: a systematic review and meta‐analysis
Authors: Narasimhan V. , Tan S. , Kong J. , Pham T. , Michael M. , Ramsay R. , Warrier S. , Heriot A. 
Published: February 2020

AbstractAimPeritoneal metastases from colorectal cancer confer the worst survival among all metastatic sites. The adoption of cytoreductive surgery (CRS) with hyperthermic intraperitoneal chemotherapy (HIPEC) can offer selected patients with isolated colorectal peritoneal metastases (CRPM) a favourable long‐term survival. There are numerous factors postulated to influence survival in patients undergoing CRS and HIPEC. The aim of this study was to identify the key perioperative prognostic factors that influence survival in patients undergoing CRS and HIPEC for isolated CRPM.MethodA systematic review and meta‐analysis were conducted to evaluate prognostic factors influencing survival in patients undergoing CRS and HIPEC for isolated CRPM.ResultsThirty‐three studies fitted the inclusion criteria for the systematic review, with 25 studies included in the meta‐analysis. On pooled analysis, incomplete cytoreduction, increasing peritoneal carcinoma index (PCI) and lymph node involvement were significantly associated with a worse survival. Additionally, a rectal primary [hazard ratio (HR) 1.93, 95% CI 1.10–3.37], adjuvant chemotherapy (HR 0.71, 95% CI 0.54–0.93) and perioperative grade III/IV morbidity (HR 1.59, 95% CI 1.17–2.16) were also found to significantly influence survival. Notably, tumour differentiation and signet ring cell histology did not influence survival on pooled analysis.ConclusionThis meta‐analysis confirms that in patients undergoing CRS and HIPEC for isolated CRPM, incomplete cytoreduction, high PCI and lymph node involvement have a negative influence on survival. In addition, a rectal primary, adjuvant chemotherapy use and grade III/IV morbidity are important factors that also significantly influence survival.
A20 inhibits osteoclastogenesis via TRAF6‐dependent autophagy in human periodontal ligament cells under hypoxia
Authors: Yan Ke , Wu Chengyu , Ye Yu , Li Lu , Wang Xiaoqian , He Wei , Ren Shuangshuang , Xu Yan 
Published: February 2020

AbstractObjectivesA20 exerts an anti‐osteoclastogenic effect through the inhibition of NF‐κB signalling in periodontitis. It also regulates autophagy via ubiquitin modification. This study was aimed at exploring the relationship between A20 and autophagy in anti‐osteoclastogenesis in human periodontal ligament cells (hPDLCs) under hypoxia.Materials and MethodsReal‐time PCR and Western blot were used to detect relative mRNA and protein levels. The formation of autophagosomes was measured by TEM. Osteoclastic differentiation was assessed by TRAP staining and hydroxyapatite resorption assay. The interactions between different proteins were observed by co‐IP.ResultsCells cultured under 2% O₂ exhibited decreased A20 expression and increased RANKL/OPG (R/O) ratio. There was a negative correlation between A20 and TRAF6, and similar results were found with autophagic flux. A20 delayed the increase in R/O ratio under hypoxia. Autophagy in hPDLCs and osteoclast differentiation and hydroxyapatite resorption areas in mouse bone marrow mononuclear cells (BMMCs) were inhibited by A20. Moreover, inhibition of autophagy using 3‐MA resulted in increased expression of A20 and decreased number and function of osteoclasts. In addition, A20 inhibited polyubiquitination at K63 and enhanced that at K48 in TRAF6 to suppress autophagy under hypoxic conditions.ConclusionsA20 inhibits osteoclastogenesis via inhibition of TRAF6‐dependent autophagy in hPDLCs under hypoxia. These findings suggest that A20 may be a key gene target during bone loss in periodontitis via TRAF6‐mediated inhibition of autophagy.
Treatment outcomes in oligometastatic non‐small‐cell lung cancer: A single‐centre experience
Authors: Akın Kabalak Pınar , Kızılgöz Derya , Yılmaz Ülkü , İnal Cengiz Tuba , Tunç Evrim , Yaman Şebnem , Gülhan Erkmen 
Published: February 2020

AbstractBackgroundEven in oligometastatic stage 4 disease, survival rates are higher when curative approaches focus on both the primary tumour and metastasis. So, we aim to analyse our results of oligometastatic disease retrospectively.MethodsIn total, data on 52 non‐small‐cell lung cancer (NSCLC) patients with limited metastasis (one to three synchronous/metachronous) were retrospectively analysed. All treatment modalities associated with various treatment modalities [surgery, chemoradiotherapy (CRT), supportive care and palliative chemotherapy] were compared in terms of survival. Curative treatment consisted of surgery or CRT (concurrent or sequential).ResultsThe median overall survival (OS) time was 35.2 ± 4.1 months. Surgery was superior to CRT in terms of OS (36.7 months vs 27.4 months, P > 0.05). Progression‐free survival was 29.4 ± 3.9 months, and survival after first progression (SAFP) was 15.6 ± 2.8 months. Patients in whom a metastasectomy was performed had significantly higher rate of SAFP as compared with those who did not have a metastasectomy (20.07 ± 3.8 months vs 7.9 ± 1.7 months P = 0.046). According to pathological type, an adenocarcinoma was associated with better SAFP than a non‐adenocarcinoma (23 ± 4.1 vs 6.4 ± 1.5, P = 0.002). The 1‐ and 2‐years OS rates were 67% and 50.4%, respectively. Among the curative treatment group, the OS of patients younger than 65 years (n = 25) was 31 months, whereas that of patients older than 65 years (n = 13) was 22 months (P = 0.88).ConclusionIn well‐selected NSCLC patients with limited metastasis, survival rates can reach up to 3 years, even in a geriatric population. Clinical N staging and co‐morbidity are important prognostic factors.
Anticonvulsant Activity of Essential Oil From Leaves of Zhumeria majdae (Rech.) in Mice: The Role of GABAA Neurotransmission and the Nitric Oxide Pathway
Authors: Aghamiri Helia , Shafaroodi Hamed , Asgarpanah Jinous 
Published: February 2020

The essential oil from the leaves of Zhumeria majdae Rech. (ZMEO) has been shown to have several beneficial effects in the clinic. In this work we examined the anticonvulsant activities of ZMEO in an experimental mouse model of seizure and aimed to identify any possible underlying mechanisms. ZMEO (5, 10, 20, and 40 mg/kg intraperitoneally (i.p.)) or diazepam, as the reference anticonvulsant drug (25, 50 and 100 µg/kg i.p.), were administered 60 minutes prior to pentylenetetrazol (PTZ) injection (intravenously (i.v.) or i.p.) and changes in threshold, latency, and frequency of clonic seizure were examined. The PTZ i.p.‐induced model of seizure was also applied for examining the protective effects of ZMEO pretreatment against PTZ‐induced mortality. In some studies, the anticonvulsant effect of the combination of diazepam and ZMEO was also studied. The protective effects of ZMEO against hindlimb tonic extensions (HLTEs) were also examined by maximal electroshock (MES) seizure testing. The γ‐aminobutyric acid (GABA)ergic mechanism and nitric oxide (NO) pathway involvement in anticonvulsant activity of ZMEO were assessed by pretreating animals with flumazenil, Nω‐nitro‐L‐arginine methyl ester (L‐NAME), aminoguanidine, and L‐arginine in a PTZ‐induced model of seizure. Administration of 20 mg/kg ZMEO significantly increased chronic seizure threshold and latency while reducing frequency of convulsions and mortality in the PTZ‐induced model. In the doses studied, ZMEO could not protect mice from HLTE and mortality induced by MES. Pretreatment with L‐arginine and diazepam potentiated the anticonvulsant effects of ZMEO, whereas pretreatment with L‐NAME, aminoguanidine, and flumazenil reversed anticonvulsant activity. The anticonvulsant activity of ZMEO may be mediated in part through a GABAergic mechanism and the NO signaling pathway.