bluuebunny/crossref_metadata_2025_split huggingface dataset:
Attenuating ischemia/reperfusion injury in rat cardiac transplantation by intracoronary infusion with siRNA cocktail solution
Authors: Yang Bo , Wang Jin , Zhao Yuanyuan , Duan Wu , Dai Chen , Han Zhenyi , Wang Meixi , Zhang Bo , Wei Lai , Chen Zhishui , Chen Dong 
Published: July 2020

Abstract
Tumor necrosis factor-α (TNF-α), caspase-8, and complement component 5a receptor (C5aR) are known to play a crucial role in the myocardial ischemia/reperfusion (I/R) injury in cardiac transplantation. We hypothesized that the intracoronary infusion of TNF-α, caspase-8, and C5aR small interfering RNAs (siRNA) would protect cardiac allograft function and improve graft survival from I/R injury-induced organ failure. I/R injury of cardiac allograft was induced by syngeneic rat cardiac transplantation, in which the transplanted hearts were infused with saline or different amounts of siRNA cocktail solution targeting TNF-α, caspase-8, and C5aR via coronary arteries, and subsequently subjected to 18 h of preservation at 4°C in histidine–tryptophan–ketoglutarate (HTK) solution. The effects of siRNA cocktail solution on prolonged cold I/R injury were determined by assessing graft survival, histopathological changes, myeloperoxidase (MPO) activity, and malondialdehyde (MDA) concentration. The perfused siRNA cocktail solution successfully knocked down the expression of TNF-α, caspase-8, and C5aR in vitro and in vivo. Approximately 91.7% of control hearts that underwent 18 h of cold ischemia ceased their function after transplantation; however, 87.5% of cardiac allografts from the highest dose siRNA cocktail solution-pretreated hearts survived &gt;14 days and exhibited minimal histological changes, with minimal cellular infiltration, interstitial edema, and inflammation and maximal reduced MPO activity and MDA concentration in the cardiac allograft. We demonstrated the feasibility and efficiency of infusion of TNF-α, caspase-8, and C5aR siRNA via the intracoronary route as a promising strategy for gene silencing against I/R injury in cardiac transplantation.
Endostatin attenuates heart failure via inhibiting reactive oxygen species in myocardial infarction rats
Authors: Xu Xuguang , Jiang Tingbo , Li Yong , Kong Liusha 
Published: July 2020

Abstract
The purpose of the present study was to evaluate whether endostatin overexpression could improve cardiac function, hemodynamics, and fibrosis in heart failure (HF) via inhibiting reactive oxygen species (ROS). The HF models were established by inducing ischemia myocardial infarction (MI) through ligation of the left anterior descending (LAD) artery in Sprague–Dawley (SD) rats. Endostatin level in serum was increased in MI rats. The decrease in cardiac function and hemodynamics in MI rats were enhanced by endostatin overexpression. Endostatin overexpression inhibited the increase in collagen I, collagen III, α-smooth muscle actin (α-SMA), connective tissue growth factor (CTGF), matrix metalloproteinase (MMP)-2 and MMP9 in the hearts of MI rats. MI-induced cardiac hypertrophy was reduced by endostatin overexpression. The increased levels of malondialdehyde (MDA), superoxide anions, the promoted NAD(P)H oxidase (Nox) activity, and the reduced superoxide dismutase (SOD) activity in MI rats were reversed by endostatin overexpression. Nox4 overexpression inhibited the cardiac protective effects of endostatin. These results demonstrated that endostatin improved cardiac dysfunction and hemodynamics, and attenuated cardiac fibrosis and hypertrophy via inhibiting oxidative stress in MI-induced HF rats.
Low circulating 25-hydroxyvitamin D level is associated with increased colorectal cancer mortality: a systematic review and dose–response meta-analysis
Authors: Wu Guanghai , Xue Mei , Zhao Yongjie , Han Youkui , Zhang Shuai , Zhang Judong , Li Chao , Xu Jing 
Published: July 2020

Abstract
Epidemiological studies have suggested inconclusive associations between 25-hydroxyvitamin D (25(OH)D) and survival in patients with colorectal cancer (CRC). The aim of the present study was to quantitatively assess these associations. PubMed, EMBASE, and Web of Science databases were systematically searched for eligible studies. Subgroup analyses based on study geographic location, publication year, length of follow-up time, sample size, and stage were conducted to explore the potential sources of heterogeneity. Dose–response relationships and pooled hazard ratios (HR) for overall and CRC-specific survival comparing the highest versus the lowest categories of circulating 25(OH)D concentrations were assessed. Overall, 17 original studies with a total of 17,770 CRC patients were included. Pooled HR (95% confidence intervals) comparing highest versus lowest categories were 0.64 (0.55–0.72) and 0.65 (0.56–0.73) for overall and CRC-specific survival, respectively. Studies conducted in the U.S.A., with median follow-up time ≥ 8 years, larger sample size, and including stage I-III patients showed a more prominent association between 25(OH)D concentrations and overall survival. The dose–response analysis showed that the risk of all-cause mortality was reduced by 7% (HR = 0.93; 95% CI: 0.90, 0.95), and the risk of CRC-specific mortality was reduced by 12% (HR = 0.88; 95% CI: 0.84, 0.93) for each 20 nmol/l increment of 25(OH)D concentration. This meta-analysis provides evidences that a higher 25(OH)D concentration is associated with lower overall mortality and CRC-specific mortality.
Relationship between lncRNA-Ang362 and prognosis of patients with coronary heart disease after percutaneous coronary intervention
Authors: Wang Hui , Gong Huichao , Liu Yingwu , Feng Limin 
Published: July 2020

Abstract
The severity and complexity evaluation of coronary artery disease in patients with coronary heart disease (CHD) require objective and accurate prognosis indexes. We assessed the relationship between lncRNA-Ang362 and prognosis of CHD patients after percutaneous coronary intervention (PCI). Clinical follow-up data of CHD patients were prospectively collected. LncRNA-Ang362 levels were detected by real-time quantitative polymerase chain reaction. Survival rate was calculated by the Kaplan–Meier method, and risk ratios and 95% confidence intervals were computed using univariate and multivariate COX proportional hazard models. Finally, 434 patients were included in the follow-up cohort. The median follow-up time was 24.8 months (6.7–40). The incidence of adverse cardiovascular events was 13.6%. The high expression group significantly tended to be smoker and higher body mass index, low-density lipoprotein cholesterol, high-sensitivity C-reactive protein, creatinine, and uric acid levels compared with the low expression group. According to the SYNTAX grade, the high-risk and medium-risk groups had significantly higher lncRNA expression levels than the low-risk group. The univariate COX regression analysis indicated that high lncRAN-Ang362 expression significantly increased the risk of adverse cardiovascular events in CHD patients after PCI (hazard risk (HR) = 3.19, 95% confidence interval (CI): 1.29–7.92). Multivariate analysis found high lncRNA-Ang362 expression was independently related to worse prognosis in CHD patients after PCI (HR = 2.83, 95%CI: 1.34–6.02). Plasma lncRNA-Ang362 may be a prognosis factor in CHD patients after PCI. The patients with higher lncRNA-Ang362 expression usually have poor prognosis.
Tratamiento quirúrgico de las fractura de pared posterior de acetábulo. Resultados a corto y mediano plazo
Authors: Villalón Ignacio E. , Cancino Jaime , Bravo Óscar , Vigueras Cristóbal , Piriz Julio , del Río Javier 
Published: July 2020

Resumen
Objetivo Evaluar los resultados radiológicos a corto y mediano plazo de pacientes operados de fractura de pared posterior de acetábulo, describir la morfología de la fractura, presencia de lesiones asociadas y la necesidad de artroplastia total de cadera (ATC) durante la evolución.
Material y métodos Estudio retrospectivo-descriptivo de una serie consecutiva de 40 pacientes operados entre julio de 2012 y julio de 2017 por fractura de pared posterior de acetábulo. Treinta y siete hombres y tres mujeres con una edad promedio de 41 años fueron operados (mediana de seguimiento 17,7 meses). Se registró el mecanismo del accidente y presencia de lesiones asociadas. Se registraron el número de fragmentos que presentaba la fractura, presencia de impactación marginal, compromiso de la cabeza femoral, presencia de fragmentos intra-articulares y complicaciones postoperatorias. Para el análisis estadístico se utilizó el test de Wilcoxon.
Resultados Todos los casos fueron secundarios a un accidente de alta energía. Un 70% de los pacientes presentó luxación posterior. La mayoría de los pacientes presentó una fractura entre 2 y 5 fragmentos (70%), 47,5% de los pacientes presentó impactación marginal, 37,5% compromiso de la cabeza femoral en zona de carga, 30% fragmentos intra-articulares. La complicación que más frecuente se observó fue la artrosis post-traumática la cual se presentó en un 22,5%. Cinco pacientes (12,5%) requirieron ATC.
Conclusión Aunque se categorizan dentro de los patrones simples, las fracturas de pared posterior son lesiones complejas. La tomografía axial computada preoperatoria es esencial para determinar lesiones intra-articulares dada su alta frecuencia y también permite realizar un buen plan preoperatorio.
Valor diagnóstico de las clasificaciones outerbridge e ICRS para la clasificación artroscópica de lesiones condrales de rodilla
Authors: Tuca María , Pineda Tomás , Oyarce Javier , Etchegaray Felix , Figueroa David 
Published: July 2020

Resumen
Objetivos Determinar el rendimiento de las clasificaciones de Outerbridge (OB) e International Cartilage Repair Society (ICRS).
Método Estudio de test diagnóstico, diseño y recolección de datos prospectivo. Siete traumatólogos sub-especialistas observaron un mismo video donde se exponían 30 lesiones condrales bajo visión artroscópica, para luego clasificarlas según OB e ICRS y consignar el tratamiento de elección para cada una de las lesiones, eligiendo entre 6 alternativas: observación, debridamiento mecánico o térmico, microfractura, OATS o terapias biológicas. Tras 7 días, los evaluadores debían repetir el mismo procedimiento.
Resultados La concordancia entre los observadores fue débil para clasificación de ICRS (k 0,25 p < 0,001) y moderada para la clasificación de OB (k 0,45 p < 0,001). La concordancia intra-observador para ICRS oscilaba entre moderada y excelente (k promedio de 0,67), y para la clasificación de OB entre buena y excelente (k promedio 0,83). Ninguna de las dos clasificaciones mostró correlación con la experiencia del cirujano. En la elección de tratamiento, la concordancia entre observadores fue débil (k 0,33 p < 0,001), sin embargo, la concordancia intra-observador fue en todos los casos buena o excelente (k 0,82), mostrando una correlación directamente proporcional a la experiencia del cirujano. La capacidad de discriminación terapéutica, evaluada mediante una regresión logística, mostró un área bajo la curva roc en el rango del no-efecto.
Conclusión Ambas clasificaciones mostraron una baja correlación inter-observador y una elevada concordancia intra-observador. En ambas categorías, Outerbridge fue más concordante que ICRS. En cuanto al tratamiento, ninguna de las dos clasificaciones logra unificar criterios quirúrgicos.
Nivel de evidencia Nivel I (test diagnóstico).
Spreader Graft for Severe Deviation of Nasal Septum with Obstruction of the Internal Nasal Valve: Clinical and Functional Results
Authors: Albergo Leandro , Desio Ernesto , Revelli Velia Elena , Acosta Micaela B. 
Published: July 2020

AbstractDifficulty in nasal ventilation is one of the most frequently occurring problems in otorhinolaryngology and its correct diagnosis is the key step to solve it. The dysfunctions in the valve area are a frequent cause of chronic nasal obstruction, though commonly ignored. The objective of the study is to analyze the clinical and functional outcomes in a group of patients with septal deviations and valve compromise treated with spreader graft with endonasal approach. Thirty-five patients with septal deviation with compromise of the internal nasal valve (INV; area II of Cottle), treated with spreader graft and a minimum follow-up of 12 months, were included for analysis. Patients were evaluated with video nasosinusal endoscopy, photography, the Nasal Obstruction Symptom Evaluation (NOSE) questionnaire, and rhinomanometry (RM). Postoperative complications were recorded. The results obtained in the pre- and postoperative NOSE scores showed significant differences (p = 0.001), as also in pre- and postoperative RM tests (p < 0.001). Two complications were reported in the 35 patients; thus the complication rate in our sample was 6%. The use of spreader grafts, with endonasal approach, as nasal septum's tutors improved perpendicular septal deviations with compromise of the INV (area II), reaching an effective functional improvement in the nasal airway, with low rate of complications.
Aplicación móvil en el diagnóstico de dolor de hombro para médicos generales
Authors: Marsalli Michael , Sepúlveda Oscar , De La Paz Joaquín 
Published: July 2020

Resumen
Objetivo Evaluar la concordancia diagnóstica durante la evaluación inicial de pacientes con dolor de hombro entre: (1) Traumatólogos especialistas en hombro realizando una evaluación convencional y (2) un médico general utilizando una nueva aplicación móvil.
Materiales y Método Se diseñó una aplicación móvil para asistir a médicos generales a realizar una evaluación del dolor de hombro entregando una hipótesis diagnóstica. Se diseñó un estudio de concordancia diagnóstica. Se evaluó un grupo de 60 pacientes, quienes consultaron por primera vez con un especialista en hombro. Primero, todos fueron evaluados por un médico general utilizando la aplicación. Se registró la hipótesis diagnóstica entregada por la aplicación. Luego, el especialista realizó una evaluación con anamnesis y examen físico, registrando su hipótesis diagnóstica. Se realizó una prueba Kappa para determinar la concordancia entre ambos evaluadores.
Resultados La concordancia global entre los especialistas y la aplicación móvil utilizada por el médico general fue sustancial (k = 0,74; p < 0,001). La concordancia fue casi perfecta para el Pinzamiento subacromial, dolor cervical, lesión acromioclavicular, inestabilidad de hombro, y el pinzamiento interno.
Conclusión La concordancia diagnóstica durante la evaluación inicial de pacientes con dolor de hombro entre traumatólogos especialistas y un médico general utilizando una aplicación móvil fue sustancial.
Nivel de Evidencia Tipo III.
Therapeutic Plasma Exchange Application in Children Requires Individual Decision
Authors: Atay Gürkan , Demirkol Demet 
Published: July 2020

AbstractTherapeutic plasma exchange (TPE) is a treatment administered with the aim of removing a pathogenic material or compound causing morbidity in a variety of neurologic, hematologic, renal, and autoimmune diseases. In this study, we aimed to assess the indications, efficacy, reliability, complications, and treatment response of pediatric patients for TPE. This retrospective study analyzed data from 39 patients aged from 0 to 18 years who underwent a total of 172 TPE sessions from January 2015 to April 2018 in a tertiary pediatric intensive care unit. Indications for TPE were, in order of frequency, macrophage activation syndrome (28.2%, n = 11), renal transplantation rejection (15.4%, n = 6), liver failure (15.4%, n = 6), Guillain–Barre's syndrome (15%, n = 6), hemolytic uremic syndrome (7.7%, n = 3), acute demyelinating disease (7.7%, n = 3), septic shock (5.1%, n = 2), and intoxication (5.1%, n = 2). No patient had any adverse event related to the TPE during the procedure. The TPE session was ended prematurely in one patient due to insufficient vascular access and lack of blood flow (2.6%). In the long term, thrombosis due to the indwelling central catheter occurred (5.1%, n = 2). TPE appears to be an effective first-stage or supplementary treatment in a variety of diseases, may be safely used in pediatric patients, and there are significant findings that its area of use will increase. In experienced hands and when assessed carefully, it appears that the rate of adverse reactions and vascular access problems may be low enough to be negligible.
Too early to say: The Englishtoo ADJ to Vconstruction and models of cross-cultural communications styles
Authors: Pavlović Vladan 
Published: July 2020

AbstractThis paper studies the Englishtoo ADJ to Vconstruction. It starts with a (multiple) distinctive collexeme analysis (as one of the subtypes of collostructional analysis) of theADJ-Vpairs appearing in the given construction in three regional varieties of English (American, British and Indian English) based on the GloWbE corpus. This analysis establishes the most distinctive and most strongly repelledADJ-Vpairs in the respective varieties. These results are then interpreted from the perspective of three models of inter-cultural and cross-cultural communication styles. The paper demonstrates that the most distinctive and the most repelledADJ-Vpairs do differ across the three varieties and that this may reflect subtle differences in the underlying cultural conceptualizations. The paper also introduces the notion of ‘(multiple) distinctive collexeme analysis of co-varying collexemes’, as an extension of the existing notion of the given type of collostructional analysis.
Tracking and analyzing recent developments in German-language online press in the face of the coronavirus crisis
Authors: Wolfer Sascha , Koplenig Alexander , Michaelis Frank , Müller-Spitzer Carolin 
Published: July 2020

Abstract
The coronavirus pandemic may be the largest crisis the world has had to face since World War II. It does not come
as a surprise that it is also having an impact on language as our primary communication tool. In this short paper, we present
three inter-connected resources that are designed to capture and illustrate these effects on a subset of the German language: An
RSS corpus of German-language newsfeeds (with freely available untruncated frequency lists), a continuously updated HTML page
tracking the diversity of the vocabulary in the RSS corpus and a Shiny web application that enables other researchers and the
broader public to explore the corpus in terms of basic frequencies.
Study of immune‐tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA‐G from semiallograft immune tolerance during pregnancy
Authors: Cho Kyoungshik , Kook Hyejin , Kang Suman , Lee Jangho 
Published: July 2020

ABSTRACTAn immune reaction is a protector of our body but a target to be overcome for all non‐self‐derived medicine. Extracellular Vesicles (EVs), noted as a primary alternative to cell therapy products that exhibit immune rejection due to mismatching‐major histocompatibility complex (MHC), were discovered to have excellent curative effects through the delivery of various biologically active substances. Although EVs are sure to incur immune reaction by immunogenicity due to alloantigens from their parental cells, their immune rejection is rarely known. Hence, to develop cell lines and EVs as medicines with no immune rejection, we noted the immune tolerance where the foetus, as semi‐allograft, is perfectly protected from the maternal immune system. We designed the ex‐vivo culture systems to simulate in‐vivo environmental factors inducing extravillous trophoblast (EVT)‐specific Human Leukocyte Antigen‐G (HLA‐G) expression and secretion of HLA‐G‐bearing EVs at the mother‐foetus interface. Using them, we confirmed that immune‐tolerized stem cells (itSCs) continuously expressing and secreting HLA‐G like EVTs during pregnancy can be induced. Also, EVs secreted from itSCs are verified as immune‐tolerized EVs (itSC‐EVs) containing HLA‐G and not causing immune rejection through various analytical methods. These findings can provide a new perspective on the local and extensive immune tolerance environment where HLA‐G is expressed and secreted by pregnancy‐related hormones and different biological conditions. Furthermore, they show the new way to develop itSCs‐EVs‐based therapeutics that are free from time, space, and donor limitation causing immune rejection.AbbreviationsCFSE: carboxyfluorescein succinimidyl ester; DC: dendritic cells; ELISA: enzyme‐linked immunosorbent assay; EV: extracellular vesicles; EVT: extravillous trophoblast; FSH: follicle stimulating hormone; HA: hyaluronic acid; hCG: human chorionic gonadotropin; HLA‐G: human leukocyte antigen G; iPSC: induced pluripotent stem cells; itSC‐EVs: immune‐tolerized extracellular vesicles from itSCs; itTBC‐EVs: immune‐tolerized extracellular vesicles from itTBCs; itSCs: immune tolerized stem cells; itTBCs: immune‐tolerized trophoblast cells; LH: luteinizing hormone; MHC: major histocompatibility complex; MSC: mesenchymal stem cells; NK: natural killer cells; NTA: nanoparticle tracking analysis; PBMC: peripheral blood mononuclear cells; PHA: phytohemagglutinin; SP‐IRIS: single particle interferometric reflectance imaging sensing; STB: syncytiotrophoblast
Higher white matter hyperintensity lesion load is associated with reduced long-range functional connectivity
Authors: Quandt Fanny , Fischer Felix , Schröder Julian , Heinze Marlene , Lettow Iris , Frey Benedikt M , Kessner Simon S , Schulz Maximilian , Higgen Focko L , Cheng Bastian , Gerloff Christian , Thomalla Götz 
Published: July 2020

Abstract
Cerebral small vessel disease is a common disease in the older population and is recognized as a major risk factor for cognitive decline and stroke. Small vessel disease is considered a global brain disease impacting the integrity of neuronal networks resulting in disturbances of structural and functional connectivity. A core feature of cerebral small vessel disease commonly present on neuroimaging is white matter hyperintensities. We studied high-resolution resting-state EEG, leveraging source reconstruction methods, in 35 participants with varying degree of white matter hyperintensities without clinically evident cognitive impairment in an observational study. In patients with increasing white matter lesion load, global theta power was increased independently of age. Whole-brain functional connectivity revealed a disrupted network confined to the alpha band in participants with higher white matter hyperintensities lesion load. The decrease of functional connectivity was evident in long-range connections, mostly originating or terminating in the frontal lobe. Cognitive testing revealed no global cognitive impairment; however, some participants revealed deficits of executive functions that were related to larger white matter hyperintensities lesion load. In summary, participants without clinical signs of mild cognitive impairment or dementia showed oscillatory changes that were significantly related to white matter lesion load. Hence, oscillatory neuronal network changes due to white matter lesions might act as biomarker prior to clinically relevant behavioural impairment.
Prediction of Finite Population Proportion When Responses are Misclassified
Authors: Adhya Sumanta , Roy Surupa , Banerjee Tathagata 
Published: July 2020

Abstract
We propose a model-based predictive estimator of the finite population proportion of a misclassified binary response, when information on the auxiliary variable(s) is available for all units in the population. Asymptotic properties of the misclassification-adjusted predictive estimator are also explored. We propose a computationally efficient bootstrap variance estimator that exhibits better performance compared to usual analytical variance estimator. The performance of the proposed estimator is compared with other commonly used design-based estimators through extensive simulation studies. The results are supplemented by an empirical study based on literacy data.