bluuebunny/crossref_metadata_2025_split huggingface dataset:
Image‐guided percutaneous cryoablation of unresectable sacrococcygeal chordoma: Feasibility and outcome in a selected group of patients with long term follow‐up
Authors: Cherix Stéphane , Traverso Aurélien , Bazan Alexander A. , Gallusser Nicolas , Heutschi‐Ozturk Hafize , Abou‐Khalil Sami , Goetti Patrick , Letovanec Igor , Montemurro Michael , Bize Pierre 
Published: November 2020

AbstractBackgroundChordoma is a rare malignant tumor of the axial skeleton. Percutaneous cryoablation (PCA) is a minimally invasive technique that allows freezing of tumors under imaging control. The purpose of our retrospective study was to investigate the outcome of PCA in a selected cohort of patients with sacrococcygeal chordoma, with a minimum of 5 years follow‐up.Materials and MethodsFour patients were treated in 10 sessions. The mean follow‐up was 57.3 months. We evaluated the feasibility, the procedure‐related complications, the impact on pain control and oncological outcomes.ResultsFreezing of 100% of the tumor volume was possible in 60%. Pain control was not reliably evaluable. Local recurrence occurred in 90% of the treated lesions; the mean time to progression was 8.1 months (range 1.5–16). At last follow‐up, one patient had died of the disease, one of another cause and one was receiving the best supportive care. The only patient alive without the disease had received additional carbon‐ion radiotherapy. The 5‐year survival rate after index PCA was 50%.ConclusionComplete freezing of the tumor was technically challenging, mainly due to the complex local anatomy. Recurrence occurred in 90% of the lesions treated. PCA should be considered with caution in the curative management of sacrococcygeal chordoma.
Influences of GABAergic Inhibition in the Dorsal Medulla on Contralateral Swallowing Neurons in Rats
Authors: Kinoshita Shota , Sugiyama Yoichiro , Hashimoto Keiko , Fuse Shinya , Mukudai Shigeyuki , Umezaki Toshiro , Dutschmann Mathias , Hirano Shigeru 
Published: November 2020

ObjectivesWe aimed to examine the effect of unilateral inhibition of the medullary dorsal swallowing networks on the activities of swallowing‐related cranial motor nerves and swallowing interneurons.MethodsIn 25 juvenile rats, we recorded bilateral vagal nerve activity (VNA) as well as unilateral phrenic and hypoglossal activity (HNA) during fictive swallowing elicited by electrical stimulation of the superior laryngeal nerve during control and following microinjection of the GABA agonist muscimol into the caudal dorsal medulla oblongata in a perfused brainstem preparation. In 20 animals, swallowing interneurons contralateral to the muscimol injection side were simultaneously recorded extracellularly and their firing rates were analyzed during swallowing.ResultsIntegrated VNA and HNA to the injection side decreased to 49.0 ± 16.6% and 32.3 ± 17.9%, respectively. However, the VNA on the uninjected side showed little change after muscimol injection. Following local inhibition, 11 out of 20 contralateral swallowing interneurons showed either increased or decreased of their respective firing discharge during evoked‐swallowing, while no significant changes in activity were observed in the remaining nine neurons.ConclusionThe neuronal networks underlying the swallowing pattern generation in the dorsal medulla mediate the ipsilateral motor outputs and modulate the contralateral activity of swallowing interneurons, suggesting that the bilateral coordination of the swallowing central pattern generator regulates the spatiotemporal organization of pharyngeal swallowing movements.Level of EvidenceNA Laryngoscope, 131:2187–2198, 2021
Influence of 9 years of permanent raised beds and contour furrowing on soil health in conservation agriculture based systems in Tigray region, Ethiopia
Authors: Araya Tesfay , Gebremedhin Alemu , Baudron Frédéric , Hailemariam Mengsteab , Birhane Emiru , Nyssen Jan , Govaerts Bram , Cornelis Wim 
Published: November 2020

AbstractConservation agriculture (CA) systems represent a set of three soil management principles: minimarbance, permanent soil cover and crop rotations ‐ whereas the CA‐based systems in this study add the bed and furrow tillage techniques as integral elements of CA. Studies on the effects of long‐term CA‐based systems on soil health and crop productivity are rare globally, particularly in Ethiopia. This study aimed at investigating the long‐term (2005–2013) influence of CA‐based systems on soil health and crop productivity in northern Ethiopia. The treatments we used include two types of CA‐based systems (permanent raised bed PRB and contour furrowing CF) and conventional tillage (CT) arranged in a randomized complete block design. Soil samples were collected at 0–10 cm soil depth to assess soil health. Piecewise structural equation modeling (PSEM) was used to analyze linkages between management practices, soil health and crop productivity. Arbuscular mycorrhizal fungi spore abundance and root colonization was higher in PRB followed by CF compared to CT (p < 0.05). Significantly different soil N of 1.4 and 0.9 g kg−1and available P of 6.7 and 4.1 ppm were recorded for PRB and CT, respectively. Higher straw and grain yield of 12 and 4.3, 10 and 3.5, 8 and 2.8 t ha−1were recorded for PRB, CF and CT, respectively. Outputs of the PSEM highlighted two pathways in which CA‐based systems contributed to improved productivity: (a) via higher density of bacteria and improved hydraulic conductivity, and (b) via higher density of fungi and increase soil organic carbon content in the topsoil. The study concludes that CA‐based systems have the potential to improve crop productivity through improved soil health.
Enhanced fallopian canal as a potential marker for temporal bone vasculitis
Authors: Fujikawa Taro , Honda Keiji , Ito Taku , Kishino Mitsuhiro , Kimura Naoki , Umezawa Natsuka , Hirano Mana , Aoki Natsuki , Kawashima Yoshiyuki , Tsutsumi Takeshi 
Published: November 2020

AbstractObjectivesThis study aimed to test the hypothesis that contrast‐enhanced 3D MRI with gradient‐echo sequences (CE‐3D‐GRE) can detect signs of vasculitis in the fallopian canal, which may cause otologic involvement, in four patients with antineutrophil cytoplasmic antibody‐associated vasculitis (AAV).MethodsCE‐3D‐GRE acquired at 3.0 Tesla was performed on four patients diagnosed with granulomatosis with polyangiitis or eosinophilic granulomatosis with polyangiitis, at onset or relapse of the disease, and in remission. Clinical correlations between otologic symptoms and radiological findings were examined for each patient. Furthermore, signal intensity of the mastoid segment of the fallopian canal was compared between the ears with active disease (n = 3) and those in remission or without vasculitis (n = 3).ResultsIntense enhancement in the tympanic and mastoid segments of the fallopian canal was associated with development of external otitis, otitis media, and sensorineural hearing loss, and was unrelated to the presence of facial paresis. Maximal intensity projection images visualized the close relationship between the enhanced fallopian canal and middle ear inflammation. The findings were absent in remission. Signal intensity of the mastoid segment of the fallopian canal was higher in ears with active disease than in normal ears (P < .001) and decreased to normal levels during remission (P = .597).ConclusionCE‐3D‐GRE can demonstrate vasculitis in the temporal bone, reflecting disease activity and the severity of otologic manifestations, including cochlear involvement, in AAV patients. Intense enhancement of the fallopian canal on CE‐3D‐GRE can be a potential marker for vasculitis of the temporal bone.Level of Evidence5.
Thermosensitive “Smart” Surfaces for Biorecognition Based Cell Adhesion and Controlled Detachment
Authors: Brunato Silvia , Mastrotto Francesca , Bellato Federica , Garofalo Mariangela , Göddenhenrich Thomas , Mantovani Giuseppe , Alexander Cameron , Gross Silvia , Salmaso Stefano , Caliceti Paolo 
Published: November 2020

AbstractThe biorecognition‐based control of attachment/detachment of MCF‐7 cancer cells from polymer‐coated surfaces is demonstrated. A glass surface is coated with a thermoresponsive statistical copolymer of poly(N‐isopropylacrylamide‐co‐acrylamide) [p(NIPAm‐co‐Am)], which is end‐capped with the Gly‐Arg‐Gly‐Asp‐Ser (GRGDS) peptide, and the hydrophilic polymer poly(ethylene glycol) (PEG). Below the lower critical solution temperature (LCST) of p(NIPAm‐co‐Am) (38 °C), the copolymers are in the extended conformation, allowing for accessibility of the GRGDS peptides to membrane‐associated integrins thus enabling cell attachment. Above the LCST, the p(NIPAm‐co‐Am) polymers collapse into globular conformations, resulting in the shielding of the GRGDS peptides into the PEG brush with consequent inaccessibility to cell‐surface integrins, causing cell detachment. The surface coating is carried out by a multi‐step procedure that included: glass surface amination with 3‐aminopropyltriethoxysilane; reaction of mPEG5kDa‐N‐hydroxysuccinimide (NHS) and p(NIPam‐co‐Am)15.1kDa‐bis‐NHS with the surface aminopropyl groups and conjugation of GRGDS to the carboxylic acid termini of p(NIPam‐co‐Am)15.1kDa‐COOH. A range of spectrophotometric, surface, and microscopy assays confirmed the identity of the polymer‐coated substrates. Competition studies prove that MCF‐7 cancer cells are attached via peptide recognition at the coated surfaces according to the mPEG5kDa/p(NIPam‐co‐Am)15.1kDa‐GRGDS molar ratio. These data suggest the system can be exploited to modulate cell integrin/GRGDS binding for controlled cell capture and release.
Characterization of Anisotropic Human Hair Keratin Scaffolds Fabricated via Directed Ice Templating
Authors: Zhao Zhitong , Moay Zi Kuang , Lai Hui Ying , Goh Bernice Huan Rong , Chua Huei Min , Setyawati Magdiel Inggrid , Ng Kee Woei 
Published: November 2020

AbstractHuman hair keratin (HHK) is successfully exploited as raw materials for 3D scaffolds for soft tissue regeneration owing to its excellent biocompatibility and bioactivity. However, most HHK scaffolds are not able to achieve the anisotropic mechanical properties of soft tissues such as tendons and ligaments due to lack of tunable, well‐defined microstructures. In this study, directed ice templating method is used to fabricate anisotropic HHK scaffolds that are characterized by aligned pores (channels) in between keratin layers in the longitudinal plane. In contrast, pores in the transverse plane maintain a homogenous rounded morphology. Channel widths throughout the scaffolds range from ≈5 to ≈15 µm and are tunable by varying the freezing temperature. In comparison with HHK scaffolds with random, isotropic pore structures, the tensile strength of anisotropic HHK scaffolds is enhanced significantly by up to fourfolds (≈200 to ≈800 kPa) when the tensile load is applied in the direction parallel to the aligned pores. In vitro results demonstrate that the anisotropic HHK scaffolds are able to support human dermal fibroblast adhesion, spreading, and proliferation. The findings suggest that HHK scaffolds with well‐defined, aligned microstructure hold promise as templates for soft tissues regeneration by mimicking their anisotropic properties.
Next‐generation Antimicrobial Peptides (AMPs) incorporated nanofibre wound dressings
Authors: Afshar Ayda , Yuca Esra , Wisdom Cate , Alenezi Hussain , Ahmed Jubair , Tamerler Candan , Edirisinghe Mohan 
Published: November 2020

AbstractAntimicrobial peptides (AMPs) containing polymer‐based nanodelivery systems offer to overcome many challenges in wound care. While preventing the contact of the external agents on the wound, it also addresses a rising concern on the drug resistance. AMPs as the host defence peptides have been increasingly recognized for therapeutic potential owning to their critical role in innate immunity. Here we investigated a nanofibre mesh approach using AMPs incorporated polyethylene oxide (PEO) for wound healing applications. PEO was prepared to carry GH12‐COOH‐M2 (type 1 AMP) and AMP2 (type 2 AMP), and their antibacterial activity was assessed againstStaphylococcus epidermidis (S. epidermidis). PEO‐AMP nanofibre meshes were successfully formed by using pressurized gyration (PG), which allows rapid mass production. Bacterial viability of the nanofibre meshes was investigated using the AlamarBlue assay. Fibre morphology, size distribution and AMP incorporation in the nanofibres were characterized by scanning electron microscopy (SEM), fluorescence microscopy (polarization contrast images) and Fourier transform infrared spectroscopy (FTIR). While both PEO‐AMP1 and PEO‐AMP2 nanofibres indicate promising bacterial inhibition at 105 µg/ml, PEO‐AMP2 fibres showed the highestS. epidermidisreduction. The results demonstrated that increase in the AMP content reduced the bacterial growth. Another important implementation of the PEO‐AMP nanofibres is that they can be tuned to rapidly releasing the peptides. Antimicrobial peptide‐loaded nanofibres represent a viable biologically active solution to next‐generation wound dressings.
Effect of nurses’ perception to workplace civility climate on nurse–physician collaboration
Authors: Hossny Eman Kamel , Sabra Hanaa Esmail 
Published: November 2020

AbstractAimsTo assess the effect of nurses’ perception to workplace civility climate on nurse–physician collaboration and determine whether the largest contributor to changing the climate of the workplace are the employees or top management.DesignThis study used non‐experimental descriptive cross‐sectional study design, which using purposive sample (139 nurses) working at Health Insurance Hospital.MethodThe current study data were collected by using self‐administered questionnaires including three tools: (a) a structured interview questionnaire includes personal data; (b) perceived workplace civility climate scale (15 items); and (c) Nurse Physician Collaboration Scale (27 items).ResultsMain result of this study reveals that there was a positive statistically significant correlation between workplace civility climate and collaboration (0.208*). Studied nurses were perceived top management with the high score (87.5) than employees (65.2).ConclusionWorkplace civility climate was demanded for well collaboration between nurses and physicians to provide high‐quality services; when nurses and physicians have increase chance to work in civility climate, combining with response to incivility and low intolerance for it, they will be more collaborated. Recommendation: Policies and procedures are very important in healthcare settings to address uncivil actions and establish a civilized climate.
A reduced‐order modified finite difference method preserving unconditional energy‐stability for the Allen–Cahn equation
Authors: Li Huanrong , Song Zhengyuan , Zhang Fuchen 
Published: November 2020

AbstractIn this paper, we mainly study a reduced‐order finite difference (FD) method with an extra modified term for the Allen–Cahn equation with a small parameter perturbation and a nonlinear term concerned with the energy function. First, using a proper orthogonal decomposition (POD) technique, we construct a set of optimal POD basis and establish a reduced‐order modified finite difference (ROMFD) scheme. Second, we prove the discrete maximum‐bound‐principle (DMBP) preserving and discrete energy‐stability (DES) preserving of the ROMFD solutions under some restrictions on the coefficient of the modified term and the time step size, in particular when the coefficient of modified term is large enough, the ROMFD scheme satisfies unconditional DMP and unconditional DES for any time step. And we analyze the error estimate of the ROMFD solution for the Allen–Cahn equation. Finally, we give some numerical tests to verify all the theoretical results of our ROMFD method, including (unconditional) DMBP‐preserving and (unconditional) DES‐preserving, and to show that the CPU running time of our ROMFD method with very few degrees of freedom is much less than that of the FD method.
Accelerated weathering analysis of jute reinforced cashewnut shell liquid modified soy based green composite
Authors: Avancha Sridevi , Behera Ajaya Kumar , Sen Ramkrishna , Adhikari Basudam 
Published: November 2020

AbstractCashew nut shell liquid (CNSL) is improved as a soy resin system when aldehyde‐based curing agent is used for crosslinking. In this work, composites were prepared by reinforcing sodium hydroxide treated jute felts with CNSL modified soy resin and characterized in terms of water resistance, mechanical properties, dynamic mechanical properties and screw drilling properties. Addition of 4 wt% CNSL in soy resin increased water resistance of the composite by 10% whereas tensile properties of composite improved by about 40%. Maximum storage modulus of CNSL based composite was found 8412 MPa as compared to 1092 MPa of jute‐soy composite without CNSL. Composites were exposed to accelerated weather and checked for dimensional stability, change in weight and mechanical properties. These composites could find wide applications in railway and automobile interiors, furniture, toysand so forth.
Lung biopsy in the diagnosis of pediatric ANCA‐associated vasculitis
Authors: Sayad Edouard , Vogel Tiphanie P. , Cortes‐Santiago Nahir , Patel Kalyani R. , McNeill David M. , Spielberg David , Silva‐Carmona Manuel 
Published: November 2020

AbstractObjectiveTo investigate pulmonary histopathologic features in a cohort of pediatric patients with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis (AAV) who underwent a lung biopsy as part of their evaluation. We report the safety and the findings of lung biopsies in this population.MethodsAfter IRB approval, we performed a retrospective chart review of all patients <18 years of age presenting to our institution with a diagnosis of pediatric AAV (pAAV) who underwent lung biopsy. We reviewed histopathologic features, serologies, the timing of biopsy, and complications.ResultsFourteen patients met inclusion criteria, nine patients with microscopic polyangiitis (MPA), and five patients with granulomatosis with polyangiitis (GPA). All patients had positive ANCA serology. 13/14 patients required admission on initial presentation for respiratory symptoms; 11/13 required respiratory support. The indication for biopsy was confirmation of diagnosis before initiating therapy in 11 patients (78%), part of the infectious evaluation in two (14%), and part of interstitial lung disease evaluation in one (7%). 11/14 (78%) biopsies had findings consistent with AAV diagnosis: 6/9 (67%) of the MPA patients compared with 5/5 (100%) of the GPA patients. The most common findings on histopathology were vascular inflammation and signs of alveolar hemorrhage. The only reported complication after lung biopsy was pneumothorax in four patients (28%).ConclusionLung biopsy had a higher diagnostic yield in GPA compared with MPA patients. In our cohort, a diagnosis of AAV could be made with clinical features and positive serology but was confirmed by lung histopathology in the majority of cases. Obtaining a lung biopsy for diagnostic purposes in pAAV should be reserved for uncertain cases where the diagnosis cannot be confirmed clinically and with serology.
Assessment of the effects of novel insecticides on green peach aphid (Myzus persicae) feeding and transmission of Turnip mosaic virus (TuMV)
Authors: Samara Rana , Lowery Thomas D , Stobbs Lorne W , Vickers Patrica M , Bittner Lori A 
Published: November 2020

AbstractBACKGROUNDLaboratory bioassays using treated leaf disks of peach were conducted to determine the efficacy of nine insecticides against the green peach aphid (GPA), Myzus persicae (Sulzer). The effects of these insecticides on aphid feeding behaviors and rates of transmission of Turnip mosaic virus (TuMV) to potted rutabaga plants were also determined.RESULTSMedian lethal concentration (LC50) values after 48 h feeding varied considerably, ranging from lows of 1.5 and 4.6 μg a.i./L for sulfoxaflor and λ‐cyhalothrin, respectively, to 97.2 and 167.9 μg a.i./L for flonicamid and spirotetramat. LC50 values were lowest and roughly equivalent for λ‐cyhalothrin (1.2) acetamiprid (2.1), sulfoxaflor (0.23) and flupyradifurone (2.3) after 72 h feeding. Electrical penetration graph (EPG) recordings showed modest effects on feeding behaviors for certain insecticides, with sulfoxaflor, spirotetramat, and acetamiprid non‐significant reduction in feeding duration and number of pathway and potential drop phases occurring during the first 5 min compared with the control. However, greenhouse experiments carried out to investigate the effect of these insecticides on rates of transmission of TuMV, which is transmitted non‐persistently by GPA, resulted in only modest non‐significant reductions in infection rates for acetamiprid, pymetrozine, λ‐cyhalothrin, and flonicamid of 27%, 23%, 20%, and 17%, respectively.CONCLUSIONAll test materials were efficacious to GPA at differing levels, and some such as sulfoxaflor and acetamiprid non‐significantly reduced the duration and number of pathways and potential drop phases of feeding within the first 5 min. None, however, resulted in significant reductions in rates of transmission of TuMV. © 2020 Her Majesty the Queen in Right of Canada. Pest Management Science © 2020 Society of Chemical Industry
Lithium modulates miR-1906 levels of mesenchymal stem cell-derived extracellular vesicles contributing to poststroke neuroprotection by toll-like receptor 4 regulation
Authors: Haupt Matteo , Zheng Xuan , Kuang Yaoyun , Lieschke Simone , Janssen Lisa , Bosche Bert , Jin Fengyan , Hein Katharina , Kilic Ertugrul , Venkataramani Vivek , Hermann Dirk M. , Bähr Mathias , Doeppner Thorsten R. 
Published: November 2020

Abstract
Lithium is neuroprotective in preclinical stroke models. In addition to that, poststroke neuroregeneration is stimulated upon transplantation of mesenchymal stem cells (MSCs). Preconditioning of MSCs with lithium further enhances the neuroregenerative potential of MSCs, which act by secreting extracellular vesicles (EVs). The present work analyzed whether MSC preconditioning with lithium modifies EV secretion patterns, enhancing the therapeutic potential of such derived EVs (Li-EVs) in comparison with EVs enriched from native MSCs. Indeed, Li-EVs significantly enhanced the resistance of cultured astrocytes, microglia, and neurons against hypoxic injury when compared with controls and to native EV-treated cells. Using a stroke mouse model, intravenous delivery of Li-EVs increased neurological recovery and neuroregeneration for as long as 3 months in comparison with controls and EV-treated mice, albeit the latter also showed significantly better behavioral test performance compared with controls. Preconditioning of MSCs with lithium also changed the secretion patterns for such EVs, modifying the contents of various miRNAs within these vesicles. As such, Li-EVs displayed significantly increased levels of miR-1906, which has been shown to be a new regulator of toll-like receptor 4 (TLR4) signaling. Li-EVs reduced posthypoxic and postischemic TLR4 abundance, resulting in an inhibition of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway, decreased proteasomal activity, and declined both inducible NO synthase and cyclooxygenase-2 expression, all of which culminated in reduced levels of poststroke cerebral inflammation. Conclusively, the present study demonstrates, for the first time, an enhanced therapeutic potential of Li-EVs compared with native EVs, interfering with a novel signaling pathway that yields both acute neuroprotection and enhanced neurological recovery.