bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mineral trioxide aggregate suppresses pro‐inflammatory cytokine expression via the calcineurin/nuclear factor of activated T cells/early growth response 2 pathway in lipopolysaccharide‐stimulated macrophages
Authors: Kuramoto M. , Kawashima N. , Tazawa K. , Nara K. , Fujii M. , Noda S. , Hashimoto K. , Nozaki K. , Okiji T. 
Published: August 2020

AbstractAimTo elucidate mechanisms by which mineral trioxide aggregate (MTA) suppresses pro‐inflammatory cytokine mRNA expression in lipopolysaccharide (LPS)‐stimulated RAW264.7 macrophages.MethodologyMineral trioxide aggregate extracts were prepared by immersing set ProRoot MTA in culture medium. RAW264.7 cells were cultured in the presence of LPS and MTA extracts. mRNA expression levels of interleukin (IL)‐1α, IL‐6, early growth response 2 (Egr2), suppressor of cytokine signalling 3 (Socs3) and IL‐10 were quantified with reverse transcription‐quantitative polymerase chain reaction. Phosphorylation of nuclear factor‐kappa B (NF‐κB) p65 in RAW264.7 cells was analysed by Western blotting. Intracellular calcium imaging was performed with Fluo‐4 AM. The activity of nuclear factor of activated T cells (NFAT) was determined by luciferase assays. Enforced expression and silencing of Egr2 in RAW264.7 cells were carried out using an expression vector and specific RNAi, respectively. In vivo kinetics of Egr2+ cells in MTA‐treated rat molar pulp tissues were examined using immunohistochemistry. Data were analysed by one‐way analysis of variance, followed by the Tukey–Kramer test (P < 0.05).ResultsExposure to MTA extracts resulted in reduced mRNA expression levels of IL‐1α and IL‐6, as well as reduced expression of phosphorylated NF‐κB, in LPS‐stimulated RAW264.7 cells. Exposure to MTA extracts induced Ca2+ influx, which was blocked by NPS2143, an antagonist of calcium‐sensing receptor (CaSR); Ca2+ influx then triggered activation of calcineurin/NFAT signalling and enhanced mRNA expression of Egr2. Enforced expression of Egr2 in RAW264.7 cells promoted the expression of both IL‐10 and Socs3. In vivo application of MTA onto rat molar pulp tissue resulted in the appearance of Egr2‐expressing cells that coexpressed CD163, a typical M2 macrophage marker.ConclusionsMineral trioxide aggregate extracts induced downregulation of IL‐1α and IL‐6 in LPS‐stimulated RAW264.7 cells via CaSR‐induced activation of calcineurin/NFAT/Egr2 signalling and subsequent upregulation of IL‐10 and Socs3.
Extract‐stent‐replace for treatment of upper baffle stenosis with pacing leads after atrial switch procedures for transposition of the great arteries: An approach to avoid “jailing” the lead
Authors: Merrill Miranda , Krebsbach Angela , Przybylowicz Ryle , Broberg Craig S. , Burch Grant , Henrikson Charles A. 
Published: August 2020

AbstractIntroductionVenous stenosis is a late complication of the atrial switch (Mustard/Senning) procedure seen in patients with transposition of the great arteries (
d‐TGA). Many atrial switch patients require cardiac implantable electronic devices (CIEDs) which further increases the incidence of venous stenosis. Stenosis of the superior limb of the systemic venous pathway (SLSVP) in the presence of CIED leads presents a management challenge. We propose a method for navigating SLSVP stenosis in atrial switch patients with CIEDs.MethodsThe pulse generator and leads were removed using standard extraction techniques. Axillary access was retained via existing leads or new access was obtained. The interventional cardiology team, via groin access, performed stent‐angioplasty of the stenotic SLSVP. After stent deployment, the axillary access wire was snared from below, guided through the stent, and pulled into a long groin sheath. A sheath was then advanced over the axillary wire and into the groin sheath creating a path for passage of leads through the stent. New leads were advanced through the axillary sheath into the heart. Leads were secured using standard techniques.ResultsAll patients had a history of d‐TGA and prior atrial switch procedures. In each case, there was stenosis of the SLSVP in the setting of a CIED lead. There were no immediate complications and there was no restenosis on follow‐up.ConclusionPost‐atrial switch patients with CIEDs can develop stenosis of the SLSVP. A collaboration between electrophysiology and interventional cardiology can allow for device extraction, stent‐angioplasty, and lead reimplantation to avoid “jailing” the leads.
Effects of sphingolipids overload on red blood cell properties in Gaucher disease
Authors: Dupuis Lucie , Chipeaux Caroline , Bourdelier Emmanuelle , Martino Suella , Reihani Nelly , Belmatoug Nadia , Billette de Villemeur Thierry , Hivert Bénédicte , Moussa Fathi , Le Van Kim Caroline , de Person Marine , Franco Mélanie 
Published: August 2020

AbstractGaucher disease (GD) is a genetic disease with mutations in the GBA gene that encodes glucocerebrosidase causing complications such as anaemia and bone disease. GD is characterized by accumulation of the sphingolipids (SL) glucosylceramide (GL1), glucosylsphingosine (Lyso‐GL1), sphingosine (Sph) and sphingosine‐1‐phosphate (S1P). These SL are increased in the plasma of GD patients and the associated complications have been attributed to the accumulation of lipids in macrophages. Our recent findings indicated that red blood cells (RBCs) and erythroid progenitors may play an important role in GD pathophysiology. RBCs abnormalities and dyserythropoiesis have been observed in GD patients. Moreover, we showed higher SL levels in the plasma and in RBCs from untreated GD patients compared with controls. In this study, we quantified SL in 16 untreated GD patients and 15 patients treated with enzyme replacement therapy. Our results showed that the treatment significantly decreases SL levels in the plasma and RBCs. The increased SL content in RBCs correlates with abnormal RBC properties and with markers of disease activity. Because RBCs lack glucocerebrosidase activity, we investigated how lipid overload could occur in these cells. Our results suggested that SL overload in RBCs occurs both during erythropoiesis and during its circulation in the plasma.
Combined pre‐conditioning with salidroside and hypoxia improves proliferation, migration and stress tolerance of adipose‐derived stem cells
Authors: He Yuan , Ma Mudi , Yan Yiguang , Chen Can , Luo Hui , Lei Wei 
Published: August 2020

AbstractOxidative stress after ischaemia impairs the function of transplanted stem cells. Increasing evidence has suggested that either salidroside (SAL) or hypoxia regulates growth of stem cells. However, the role of SAL in regulating function of hypoxia‐pre–conditioned stem cells remains elusive. Thus, this study aimed to determine the effect of SAL and hypoxia pre‐conditionings on the proliferation, migration and tolerance against oxidative stress in rat adipose‐derived stem cells (rASCs). rASCs treated with SAL under normoxia (20% O2) or hypoxia (5% O2) were analysed for the cell viability, proliferation, migration and resistance against H2O2‐induced oxidative stress. In addition, the activation of Akt, Erk1/2, LC3, NF‐κB and apoptosis‐associated pathways was assayed by Western blot. The results showed that SAL and hypoxia treatments synergistically enhanced the viability (fold) and proliferation of rASCs under non‐stressed conditions in association with increased autophagic flux and activation of Akt, Erk1/2 and LC3. H2O2‐induced oxidative stress, cytotoxicity, apoptosis, autophagic cell death and NF‐κB activation were inhibited by SAL or hypoxia, and further attenuated by the combined SAL and hypoxia pre‐treatment. The SAL and hypoxia pre‐treatment also enhanced the proliferation and migration of rASCs under oxidative stress in association with Akt and Erk1/2 activation; however, the combined pre‐treatment exhibited a more profound enhancement in the migration than proliferation. Our data suggest that SAL combined with hypoxia pre‐conditioning may enhance the therapeutic capacity of ASCs in post‐ischaemic repair.
Fiber‐reinforced composite fixed dental prosthesis using an additive manufactured silicone index
Authors: Gonzalez Ana M. , Piedra‐Cascón Wenceslao , Zandinejad Amirali , Revilla‐León Marta 
Published: August 2020

AbstractObjectiveDigital tools such as facial and intraoral digitizers and additive manufacturing (AM) technologies assist restorative treatments. The objective of the present manuscript was to describe a workflow procedure for treatment planning and fabricating a fiber‐reinforced composite fixed dental prosthesis (FDP) replacing an absent maxillary lateral incisor, using additively manufactured silicone indices to facilitate the clinical intervention.Clinical considerationsThe elaboration of a direct fiber‐reinforced composite restoration is a technique sensitive procedure which might be time‐consuming for the clinician. The digital waxing helped to determine the exact position and size of the lingual wings and connectors of the fiber‐reinforced FDP and to design a three‐piece index. And the AM of the index helped to transfer the information to the patient's dentition accurately.ConclusionsThe protocol minimizes the time of clinical intervention by facilitating the transference of the virtual diagnostic waxing teeth into the patient's mouth. The three‐piece silicone index provides an individualized path of insertion of each index part while also providing a customized space and location of the lingual wings of the restoration.Clinical significanceThe usage of AM silicone indices facilitates the clinical intervention by translating the size and position of the diagnostic wax‐up teeth into the patient's mouth, minimizing clinical procedure's time.
Knockin’ on heaven’s door: Molecular mechanisms of neuronal tau uptake
Authors: De La‐Rocque Samantha , Moretto Edoardo , Butnaru Ioana , Schiavo Giampietro 
Published: August 2020

AbstractSince aggregates of the microtubule‐binding protein tau were found to be the main component of neurofibrillary tangles more than 30 years ago, their contribution to neurodegeneration in Alzheimer's disease (AD) and tauopathies has become well established. Recent work shows that both tau load and its distribution in the brain of AD patients correlate with cognitive decline more closely compared to amyloid plaque deposition. In addition, the amyloid cascade hypothesis has been recently challenged because of disappointing results of clinical trials designed to treat AD by reducing beta‐amyloid levels, thus fuelling a renewed interest in tau. There is now robust evidence to indicate that tau pathology can spread within the central nervous system via a prion‐like mechanism following a stereotypical pattern, which can be explained by the trans‐synaptic inter‐neuronal transfer of pathological tau. In the receiving neuron, tau has been shown to take multiple routes of internalisation, which are partially dependent on its conformation and aggregation status. Here, we review the emerging mechanisms proposed for the uptake of extracellular tau in neurons and the requirements for the propagation of its pathological conformers, addressing how they gain access to physiological tau monomers in the cytosol. Furthermore, we highlight some of the key mechanistic gaps of the field, which urgently need to be addressed to expand our understanding of tau propagation and lead to the identification of new therapeutic strategies for tauopathies.
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Sleep Well Be Well: Pilot of a digital intervention to improve child behavioural sleep problems
Authors: Hiscock Harriet , Ng Olivia , Crossley Louise , Chow Jennifer , Rausa Vanessa , Hearps Stephen 
Published: August 2020

AimTo investigate whether a digital sleep intervention improves child and care giver sleep and psychosocial outcomes.MethodsA total of 120 families with children aged 2–13 years, reporting moderate to severe child behavioural sleep problems, were recruited from a hospital sleep clinic waitlist or the community. Children from non‐English speaking families, with known intellectual disability (IQ < 70) or severe medical problems excluded. Tailored behavioural sleep strategies were delivered to primary care givers via a smart phone app and complementary website. Eligible families completed a baseline questionnaire and child ‘sleep check’ then received the digital sleep intervention for 5 weeks, and then completed a post questionnaire. Outcomes: care giver report of child sleep as no/mild versus moderate/severe problem over past month (primary outcome); problem child sleep patterns (Brief Infant Sleep Questionnaire or Child Sleep Habits Questionnaire), child temperament, care giver mental health (Kessler 6), care giver sleep, health service use for their child's sleep and time off work/activities to access services.ResultsAt follow up, care givers reported fewer moderate/severe child sleep problems (84.6–40.7%), improved problem child sleep patterns, better temperament and improved care giver mental health. Care giver sleep quality and quantity remained unchanged. Health service use (averaged over a 6‐month period pre‐ and post‐intervention) fell from 18.9% pre‐ to 14.1% post‐intervention.ConclusionA digital sleep intervention appears promising in improving sleep in children with moderate/severe behavioural sleep problems, and care giver mental health. It may be a useful alternative to face‐to‐face management of behavioural sleep problems.
Genetic diversity of Puccinia kuehnii, the causal agent of orange rust of sugarcane, from Brazil
Authors: Urashima Alfredo Seiiti , Mistura Tatiane de Fátima , Porto Lis Natali Rodrigues , Austin Paige Dariel , Arias Renee Silvia 
Published: August 2020

AbstractThe use of resistant genotypes is the preferred method to control orange rust of sugarcane (Saccharum spp) caused by Puccinia kuehnii. This approach has been adopted in Brazil but outbreaks of the disease on previously resistant varieties showed that the efficacy of this method is limited and requires a better understanding of pathogen diversity. Nevertheless, adequate molecular markers for examining pathogen diversity at population level are not available, which limits the success of orange rust control by genetic resistance. Therefore, two independent investigations were conducted to examine genetic diversity of P. kuehnii from São Paulo state, the most important sugarcane growing state of Brazil. First, simple‐sequence repeat (SSR) markers were developed in the present work and genotypic diversity of orange rust isolates from different locations investigated. Second, phenotypic diversity was examined by the single‐pustule inoculation technique on P. kuehnii isolates retrieved from three susceptible commercial sugarcane cultivars. A total of 96 SSR markers were generated and tested for this species. Subsequently, 29 isolates of P. kuehnii were fingerprinted with nine SSR markers to estimate the genotypic diversity by neighbour‐joining and 3D principal coordinates. The 29 isolates of the pathogen clustered into four main groups, which were identified by three SSR markers (NPRL_PK_108a, NPRL_PK_162_spka and NPRL_PK_221_spka). Phenotypic data at 21 days after the single‐pustule inoculation showed that P. kuehnii from highly susceptible commercial cultivars harboured a small proportion of variants capable of causing disease on resistant cultivars. A differential reaction was demonstrated for the most virulent variant in a repeated experiment confirming the existence of races within P. kuehnii in Brazil.
The large, diverse, and robust arsenal of Ralstonia solanacearum type III effectors and their in planta functions
Authors: Landry David , González‐Fuente Manuel , Deslandes Laurent , Peeters Nemo 
Published: August 2020

AbstractThe type III secretion system with its delivered type III effectors (T3Es) is one of the main virulence determinants of Ralstonia solanacearum, a worldwide devastating plant pathogenic bacterium affecting many crop species. The pan‐effectome of the R. solanacearum species complex has been exhaustively identified and is composed of more than 100 different T3Es. Among the reported strains, their content ranges from 45 to 76 T3Es. This considerably large and varied effectome could be considered one of the factors contributing to the wide host range of R. solanacearum. In order to understand how R. solanacearum uses its T3Es to subvert the host cellular processes, many functional studies have been conducted over the last three decades. It has been shown that R. solanacearum effectors, as those from other plant pathogens, can suppress plant defence mechanisms, modulate the host metabolism, or avoid bacterial recognition through a wide variety of molecular mechanisms. R. solanacearum T3Es can also be perceived by the plant and trigger immune responses. To date, the molecular mechanisms employed by R. solanacearum T3Es to modulate these host processes have been described for a growing number of T3Es, although they remain unknown for the majority of them. In this microreview, we summarize and discuss the current knowledge on the characterized R. solanacearum species complex T3Es.
Pan‐Islamic ideals and national loyalties: Competing attachments amongst early Muslim activists in France
Authors: Dazey Margot 
Published: August 2020

AbstractIslamist movements are often considered the epitomes of transnational movements; however, little is known about the concrete workings of their transnational ambitions. In investigating the evolution of Muslim activists in France from the late 1970s to the early 1990s, this article shows that their embrace of pan‐Islamic ideals initially conflicted with strong investment in (Arab) homeland politics. Later on, their engagement with a French Islam signalled less the emergence of a de‐territorialised, de‐culturalised Islamic identity than it did the assertion of new nationally bounded (French) attachments. Overall, the analysis sheds light on a stimulating puzzle regarding cosmopolitanism: the persistence of national forms of identification in movements that aspire to bypass national affiliations.
Rank‐based Tests for Cross‐sectional Dependence in Large (N, T) Fixed Effects Panel Data Models
Authors: Feng Long , Ding Yanling , Liu Binghui 
Published: August 2020

AbstractMost existing methods for testing cross‐sectional dependence in fixed effects panel data models are actually conducting tests for cross‐sectional uncorrelation, which are not robust to departures of normality of the error distributions as well as nonlinear cross‐sectional dependence. To this end, we construct two rank‐based tests for (static and dynamic) fixed effects panel data models, based on two very popular rank correlations, that is, Kendall's tau and Bergsma–Dassios’ τ*, respectively, and derive their asymptotic distributions under the null hypothesis. Monte Carlo simulations demonstrate applicability of these rank‐based tests in large (N,T) case, and also the robustness to departures of normality of the error distributions and nonlinear cross‐sectional dependence.
One‐year results of 0.01% atropine with orthokeratology (AOK) study: a randomised clinical trial
Authors: Tan Qi , Ng Alex LK , Choy Bonnie NK , Cheng George PM , Woo Victor CP , Cho Pauline 
Published: August 2020

AbstractPurposeTo report the 1‐year results of an investigation into whether there is an additive effect between 0.01% atropine and orthokeratology (ortho‐k), in a single‐masked, two‐arm, randomised controlled trial: Combined Atropine with Orthokeratology (AOK) for myopia control study (ClinicalTrials.gov number: NCT02955927).MethodsChinese children aged between 6 and 11 years with 1.00–4.00 D of myopia, astigmatism <2.50 D, and no more than 1.00 D anisometropia, were randomly assigned either to an AOK group or ortho‐k only (OK) group at a 1:1 ratio. Subjects in the AOK group instilled one drop of 0.01% atropine into each eye, 10 min before nightly wear of ortho‐k lenses. The primary outcome, axial elongation, was examined at 6‐monthly intervals, along with secondary outcomes including best‐corrected visual acuity (BCVA), manifest refraction, accommodation, pupil size, and corneal topography.Results29 AOK and 30 OK subjects completed the 1‐year visit. The overall axial elongation rate was significantly slower in the AOK group than in the OK group (mean (S.D.), 0.07 (0.16) mm vs 0.16 (0.15) mm, respectively; p = 0.03). A significant between‐group difference in axial elongation was observed over the first 6‐month period only (p < 0.001), but not over the second period (p = 0.818). At the 1‐year visit, increases in mean (S.D.) mesopic and photopic pupil sizes in the AOK group were 0.64 (0.48) mm and 0.36 (0.34) mm, respectively, which were significantly higher than 0.10 (0.50) mm and 0.02 (0.28) mm in the OK group (p < 0.001). At the 6‐month visit, a significant moderate negative correlation was found between axial elongation and the increase in photopic pupil size (r = −0.42, p = 0.02) in the AOK group.ConclusionsThere is an additive effect between 0.01% atropine and ortho‐k over one year, with mean axial elongation in the AOK group 0.09 mm slower than that in the OK group. It appears that the additive effect was only during the first six months; a second‐year investigation is warranted to determine whether the effect is sustained over time.
H syndrome with a novel homozygous SLC29A3 mutation in two sisters
Authors: Demir Damla , Aktaş Karabay Ezgi , Sözeri Betül , Gürsoy Fatıma , Akgün Doğan Özlem , Topaktaş Eylem , Zindancı İlkin 
Published: August 2020

AbstractH syndrome (OMIM 602782) is a recently defined autosomal recessive genodermatosis. Cutaneous findings of H syndrome include hyperpigmentation, hypertrichosis, and induration, while hearing loss, heart anomalies, hepatomegaly, hypogonadism, hyperglycemia (diabetes mellitus), low height (short stature), hallux valgus (flexion contractures), and hematological abnormalities are the extracutaneous abnormalities. We report a novel homozygous missense mutation, c.416T > C p.(Leu139Pro), in the SLC29A3 (NM_001174098.1) gene in two sisters with H syndrome presenting with different phenotypes.