bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neutrophil-Derived S100A8/A9 Amplify Granulopoiesis After Myocardial Infarction
Authors: Sreejit Gopalkrishna , Abdel-Latif Ahmed , Athmanathan Baskaran , Annabathula Rahul , Dhyani Ashish , Noothi Sunil K. , Quaife-Ryan Gregory A. , Al-Sharea Annas , Pernes Gerard , Dragoljevic Dragana , Lal Hind , Schroder Kate , Hanaoka Beatriz Y. , Raman Chander , Grant Maria B. , Hudson James E. , Smyth Susan S. , Porrello Enzo R. , Murphy Andrew J. , Nagareddy Prabhakara R. 
Published: January 2020

Background:
Myocardial infarction (MI) triggers myelopoiesis, resulting in heightened production of neutrophils. However, the mechanisms that sustain their production and recruitment to the injured heart are unclear.

Methods:
Using a mouse model of the permanent ligation of the left anterior descending artery and flow cytometry, we first characterized the temporal and spatial effects of MI on different myeloid cell types. We next performed global transcriptome analysis of different cardiac cell types within the infarct to identify the drivers of the acute inflammatory response and the underlying signaling pathways. Using a combination of genetic and pharmacological strategies, we identified the sequelae of events that led to MI-induced myelopoiesis. Cardiac function was assessed by echocardiography. The association of early indexes of neutrophilia with major adverse cardiovascular events was studied in a cohort of patients with acute MI.

Results:
Induction of MI results in rapid recruitment of neutrophils to the infarct, where they release specific alarmins, S100A8 and S100A9. These alarmins bind to the Toll-like receptor 4 and prime the nod-like receptor family pyrin domain-containing 3 inflammasome in naïve neutrophils and promote interleukin-1β secretion. The released interleukin-1β interacts with its receptor (interleukin 1 receptor type 1) on hematopoietic stem and progenitor cells in the bone marrow and stimulates granulopoiesis in a cell-autonomous manner. Genetic or pharmacological strategies aimed at disruption of S100A8/A9 and their downstream signaling cascade suppress MI-induced granulopoiesis and improve cardiac function. Furthermore, in patients with acute coronary syndrome, higher neutrophil count on admission and after revascularization correlates positively with major adverse cardiovascular disease outcomes.

Conclusions:
Our study provides novel evidence for the primary role of neutrophil-derived alarmins (S100A8/A9) in dictating the nature of the ensuing inflammatory response after myocardial injury. Therapeutic strategies aimed at disruption of S100A8/A9 signaling or their downstream mediators (eg, nod-like receptor family pyrin domain-containing 3 inflammasome, interleukin-1β) in neutrophils suppress granulopoiesis and may improve cardiac function in patients with acute coronary syndrome.
The Intricate Interplay of Spatial Attention and Expectation: a Multisensory Perspective
Authors: Zuanazzi Arianna , Noppeney Uta 
Published: January 2020

Abstract
Attention (i.e., task relevance) and expectation (i.e., signal probability) are two critical top-down mechanisms guiding perceptual inference. Attention prioritizes processing of information that is relevant for observers’ current goals. Prior expectations encode the statistical structure of the environment. Research to date has mostly conflated spatial attention and expectation. Most notably, the Posner cueing paradigm manipulates spatial attention using probabilistic cues that indicate where the subsequent stimulus is likely to be presented. Only recently have studies attempted to dissociate the mechanisms of attention and expectation and characterized their interactive (i.e., synergistic) or additive influences on perception. In this review, we will first discuss methodological challenges that are involved in dissociating the mechanisms of attention and expectation. Second, we will review research that was designed to dissociate attention and expectation in the unisensory domain. Third, we will review the broad field of crossmodal endogenous and exogenous spatial attention that investigates the impact of attention across the senses. This raises the critical question of whether attention relies on amodal or modality-specific mechanisms. Fourth, we will discuss recent studies investigating the role of both spatial attention and expectation in multisensory perception, where the brain constructs a representation of the environment based on multiple sensory inputs. We conclude that spatial attention and expectation are closely intertwined in almost all circumstances of everyday life. Yet, despite their intimate relationship, attention and expectation rely on partly distinct neural mechanisms: while attentional resources are mainly shared across the senses, expectations can be formed in a modality-specific fashion.
Use of Mini Clinical Evaluation Exercise as a Tool to Assess the Orthodontic Postgraduate Students
Authors: Jamenis Shilpa Chawla , Pharande Shilpa , Potnis Sheetal , Kapoor Prakhar 
Published: January 2020

Introduction: The mini CEX (Clinical Evaluation Exercise) is a workplace-based assessment tool intended to assess the clinical performance and a range of other skills such as communication skills, clinical judgment, professionalism, efficiency, and the overall clinical care while directly observing a real patient encounter. The clinical sessions are followed by structured feedback sessions between the assessors and the trainees. Mini CEX focuses on the actual performance and not just knowledge. This leads to better learning as well as helps to develop and strengthen the doctor–patient bond. Materials and Methods: 8 staff members and 9 orthodontic postgraduate students volunteered to participate in this study after the orientation session was taken to sensitize them about the same. An informed consent was taken from all of them. All the students had 2 sessions with all the assessors. As per the protocol of Mini CEX, immediate feedback about their performance was given to the students after each session. Feedback was also obtained from the assessors and the students after the completion of the exercise. This feedback allowed the assessors and the students to give their views on the exercise. Result: Mean scores of all the students significantly improved in the second session as compared to the first encounter. This was attributed to the feedback which they obtained from the assessors immediately after the session, which helped them improvise on various aspects of patient care. The students themselves felt that the exercise helped them learn better. Conclusion: Mini CEX can hence be considered as a valid formative assessment tool which when routinely used could enhance the learning experience, improve the communication skills of the students, as well as improve the doctor–patient bond.
Kinematic Analysis of Combined Suture-Button and Suture Anchor Augment Constructs for Ankle Syndesmosis Injuries
Authors: Wood Addison R. , Arshad Seyed A. , Kim Hannah , Stewart Donald 
Published: January 2020

Background: Syndesmosis injuries are common, with up to 25% of all ankle injuries being reported to involve an associated syndesmosis injury. These injuries are typically treated with cortical screw fixation or suture-button implants when indicated, but the addition of a suture anchor augment implant has yet to be evaluated. The purpose of this study was to evaluate the ability of a suture anchor augment to add sagittal plane translational and transverse plane rotational constraint to suture-button constructs with syndesmosis injuries. We hypothesized that the suture anchor augment oriented in parallel with the fibers of an injured anterior-inferior tibiofibular ligament (AITFL) in addition to a suture-button construct would achieve physiological motion and stability at the syndesmosis through increased rotational and translational constraint of the fibula. Methods: Eleven fresh-frozen cadaver ankles were stressed in external rotation using a custom-made ankle rig. Each ankle had simultaneous recording of ultrasound video, 6 degrees-of-freedom kinematics of the fibula and tibia, and torque as the ankle was stressed by an examiner. The ankles were tested in 6 different states: native uninjured; injured with interosseous ligament and AITFL sectioned; 1× suture button; 2× suture buttons, divergent; 1× suture anchor augment with 2× suture buttons, divergent; and 1× suture anchor augment with 1× suture buttons. Results: Only the suture anchor augment + 2× suture buttons and suture anchor augment + 1× suture-button constructs were found to be significantly different from the injured state ( P = .0003, P = .002) with mean external rotation of the fibula. Conclusion: Overall, the most important finding of this study was that the addition of a suture anchor augment to suture-button constructs provided a mechanism to increase external rotational constraint of the fibula. Clinical Relevance: This study provides a mechanistic understanding of how the combined suture-button and suture anchor augment construct provides an anatomically similar reconstruction of constraints found in the native ankle. However, none of the constructs examined in this study were able to fully restore physiologic motion.
The Role of the Academy and HRD in the Changing of the Guard
Authors: Rosenbusch Katherine , Morrison Emily 
Published: January 2020

The ProblemLife for faculty members is becoming more complex and stressful due to the rate of change and the ever-increasing demands being placed on them: publish in top journals, apply for funding, teach and support more students, integrate new technology into classes, serve on committees, and contribute to your discipline and professional association. Current systems, policies, and practices within higher education often fail to recognize the unique contributions of faculty members, especially those who might not reflect the traditional perspectives that have been put in place for decades. Rather, there remains a strongly guarded tradition of what is valued and rewarded within higher education. Hence, higher education needs to transform its organizational culture, valuing individual contributions, meeting workforce demands, and ensuring the health of the education system and society.The SolutionThe field of human resource development (HRD) with its interdisciplinary roots and expertise in learning, leadership, organizational change, and culture is uniquely poised to not only inform this conversation, but also to critique and contribute to the transformation of faculty work and associated expectations. This Issue addresses and identifies where changes are needed at the individual, organizational, and societal levels to transform the role of faculty members so that it is better aligned with the current environment and context of higher education.The StakeholdersScholars, practitioners, administrators, and community who recognize that the health of the broader community is affected by the quality of higher education and, in particular, faculty and the way that they teach and prepare students to be scholar-practitioners.
Chemical surveillance in freshwaters: small sample sizes underestimate true pollutant loads and fail to detect environmental quality standard exceedances
Authors: Babitsch Denise , Sundermann Andrea 
Published: January 2020

Abstract
Background
Chemical surveillance in surface waters is crucial to identify potential threats to the health of freshwater ecosystems. Usually, the concentrations of pollutants are highly variable over the course of the year and often result in non-normally distributed data sets. Therefore, the European Water Framework Directive recommends measuring, e.g. priority substances at least 12 times a year to achieve an acceptable accuracy level for the estimation of the true mean annual loads. However, in Europe priority substances are often measured much less frequently. In this context, the aim of the present study was to analyze how sample size, temporal variability and skewness of the data sets influence the accuracy of the mean annual load estimation and the assessment of annual average environmental quality standards. For this purpose, sample size simulations using weekly composite samples of benzo(a)pyrene, 4-tert-octylphenol, fluoranthene and di(2-ethylhexyl) phthalate, selected as representatives for priority substances, were carried out.

Results
The sample size simulations showed two general patterns: the accuracy of the mean annual load estimation increased with increasing sample size and skewness and temporal variability were more apparent in smaller sample sizes. In right-skewed data sets, small sample sizes led, on average, to a systematic underestimation of the true mean annual load whilst in a few cases these led to an overestimation. Although the study was carried out on priority substances, results can be transferable to other pollutants. Furthermore, in small sample sizes a considerable proportion of the simulated means failed to detect annual average environmental quality standard exceedances.

Conclusions
The results of the present study indicate that the usage of small sample sizes is likely to result in an underestimation of the true mean annual pollutant loads in chemical surveillance and scientific research, thus potentially jeopardizing the validity of results. Therefore, it is recommended to avoid the usage of small sample sizes for the determination of mean annual pollutant loads. Furthermore, priority substances should be sampled according to the European Water Framework Directive guidelines at least 12 times/year to improve the assessment of the threat posed by pollutants to freshwater ecosystems in Europe.
Mining of favorable alleles for seed reserve utilization efficiency in Oryza sativa by means of association mapping
Authors: Ali Nour , Li Dalu , Eltahawy Moaz S. , Abdulmajid Dina , Bux Lal , Liu Erbao , Dang Xiaojing , Hong Delin 
Published: January 2020

AbstractBackgroundWet direct-seeded rice is a possible alternative to conventional puddled transplanted rice; the former uses less water and reduces labor requirements. Improving seed reserve utilization efficiency (SRUE) is a key factor in facilitating the application of this technology. However, the QTLs controlling this trait are poorly investigated. In this study, a genome-wide association study (GWAS) was conducted using a natural population composed of 542 accessions of rice (Oryza sativaL.) which were genotyped using 266 SSR markers. Large phenotypic variations in SRUE were found in the studied population.ResultsThe average SRUE over 542 accessions across two years (2016 and 2017) was 0.52 mg.mg− 1, ranging from 0.22 mg.mg-1to 0.93 mg.mg− 1, with a coefficient of variation of 22.66%. Overall, 2879 marker alleles were detected in the population by 266 pairs of SSR markers, indicating a large genetic variation existing in the population. Using general linear model method, 13 SSR marker loci associated with SRUE were detected and two (RM7309 and RM434) of the 13 loci, were also detected using mixed linear model analyses, with percentage of phenotypic variation explained (PVE) greater than 5% across two years. The 13 association loci (P < 0.01) were located on all chromosomes except chromosome 11, with PVE ranging from 5.05% (RM5158 on chromosome 5) to 12% (RM297 on chromosome 1). Association loci RM7309 on chromosome 6 and RM434 on chromosome 9 revealed by both models were detected in both years. Twenty-three favorable alleles were identified with phenotypic effect values (PEV) ranging from 0.10 mg.mg− 1(RM7309–135 bp on chromosome 9) to 0.45 mg.mg− 1(RM297–180 bp on chromosome 2). RM297–180 bp showed the largest phenotypic effect value (0.44 mg.mg− 1in 2016 and 0.45 mg.mg− 1in 2017) with 6.72% of the accessions carrying this allele and the typical carrier accession was Manyedao, followed by RM297–175 bp (0.43 mg.mg− 1in 2016 and 0.44 mg.mg− 1in 2017).ConclusionNine novel association loci for SRUE were identified, compared with previous studies. The optimal parental combinations for pyramiding more favorable alleles for SRUE were selected and could be used for breeding rice accessions suitable for wet direct seeding in the future.
SMRT sequencing of a full-length transcriptome reveals transcript variants involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in Pennisetum giganteum
Authors: Li Qingyuan , Xiang Conglin , Xu Lin , Cui Jinghua , Fu Shao , Chen Baolin , Yang Shoukun , Wang Pan , Xie Yanfeng , Wei Ming , Wang Zhanchang 
Published: January 2020

Abstract
Background
Pennisetum giganteum, an abundant, fast-growing perennial C4 grass that belongs to the genus Pennisetum, family Poaceae, has been developed as a source of biomass for mushroom cultivation and production, as a source of forage for cattle and sheep, and as a tool to remedy soil erosion. However, having a chilling-sensitive nature, P. giganteum seedlings need to be protected while overwintering in most temperate climate regions.

Results
To elucidate the cold stress responses of P. giganteum, we carried out comprehensive full-length transcriptomes from leaf and root tissues under room temperature (RT) and chilling temperature (CT) using PacBio Iso-Seq long reads. We identified 196,124 and 140,766 full-length consensus transcripts in the RT and CT samples, respectively. We then systematically performed functional annotation, transcription factor identification, long non-coding RNAs (lncRNAs) prediction, and simple sequence repeat (SSR) analysis of those full-length transcriptomes. Isoform analysis revealed that alternative splicing events may be induced by cold stress in P. giganteum, and transcript variants may be involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in P. giganteum. Furthermore, the fatty acid composition determination and gene expression level analysis supported that C18 unsaturated fatty acid biosynthesis and metabolism pathways may play roles during cold stress in P. giganteum.

Conclusions
We provide the first comprehensive full-length transcriptomic resource for the abundant and fast-growing perennial grass Pennisetum giganteum. Our results provide a useful transcriptomic resource for exploring the biological pathways involved in the cold stress responses of P. giganteum.
Genome-wide survey and expression analysis of calcium-dependent protein kinase (CDPK) in grass Brachypodium distachyon
Authors: Wen Feng , Ye Feng , Xiao Zhulong , Liao Liang , Li Tongjian , Jia Mingliang , Liu Xinsheng , Wu Xiaozhu 
Published: January 2020

Abstract
Background
Ca2+ played as a ubiquitous secondary messenger involved in plant growth, development, and responses to various environmental stimuli. Calcium-dependent protein kinases (CDPK) were important Ca2+ sensors, which could directly translate Ca2+ signals into downstream phosphorylation signals. Considering the importance of CDPKs as Ca2+ effectors for regulation of plant stress tolerance and few studies on Brachypodium distachyon were available, it was of interest for us to isolate CDPKs from B. distachyon.

Results
A systemic analysis of 30 CDPK family genes in B. distachyon was performed. Results showed that all BdCDPK family members contained conserved catalytic Ser/Thr protein kinase domain, autoinhibitory domain, and EF-hand domain, and a variable N-terminal domain, could be divided into four subgroup (I-IV), based upon sequence homology. Most BdCDPKs had four EF-hands, in which EF2 and EF4 revealed high variability and strong divergence from EF-hand in AtCDPKs. Synteny results indicated that large number of syntenic relationship events existed between rice and B. distachyon, implying their high conservation. Expression profiles indicated that most of BdCDPK genes were involved in phytohormones signal transduction pathways and regulated physiological process in responding to multiple environmental stresses. Moreover, the co-expression network implied that BdCDPKs might be both the activator and the repressor involved in WRKY transcription factors or MAPK cascade genes mediated stress response processes, base on their complex regulatory network.

Conclusions
BdCDPKs might play multiple function in WRKY or MAPK mediated abiotic stresses response and phytohormone signaling transduction in B. distachyon. Our genomics analysis of BdCDPKs could provide fundamental information for further investigation the functions of CDPKs in integrating Ca2+ signalling pathways in response to environments stresses in B. distachyon.
Clinical outcomes of prophylactic compression sutures for treatment of uterine atony during the cesarean delivery of twins
Authors: Kim Mi-La , Hur Yoon-Mi , Ryu Hyejin , Lee Min Jin , Seong Seok Ju , Shin Joong Sik 
Published: January 2020

Abstract
Background
Twin pregnancy has a high risk for developing uterine atony (UA). This study aimed to evaluate efficacy and clinical outcomes of prophylactic compression sutures to treat UA during twin cesarean section (CS).

Methods
All patient records of twin deliveries by CS after gestational age of 24 weeks in a large maternity hospital in South Korea between January 2013 and June 2018 were reviewed. Patients with monochorionic monoamniotic twins were excluded from data analysis. In total, 953 women were eligible for data analysis.

Results
Of the 953 patients, compression sutures were applied to 147 cases with postpartum bleeding that were refractory to uterine massage and uterotonics. Out of the 147, two patients (1.4%) proceeded to additional uterine artery ligation to achieve hemostasis, yielding a success rate of 98.6%. The rate of transfusion after the first 24 h of delivery in the suture group was not significantly different from that in the non-suture group, suggesting that both groups achieved hemostasis at an equal rate after the first 24 h of delivery. The difference in the operation time between the two groups was only 8.5 min. The rate of subsequent pregnancy among the patients who received compression sutures was 44.4%.

Conclusions
Overall, our findings suggest that with early and fast implementation of compression sutures, UA can be treated in the setting of twin cesarean delivery without significantly increasing maternal morbidity.