bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Genomic Analysis of an Apiotrichum cacaoliposimilis Strain Isolated from a Patient with Urinary Tract Infection
Authors: Wang Wei , Yi Jinping , Zhan Jiahuan , Luo Dong , Chen Qiang , Yu Shengming , Xie Ling , Chen Kaisen 
Published: December 2024

Abstract
Opportunistic infections caused by fungi, particularly those occurring in immunocompromised patients, are considered challenging worldwide. Therefore, a comprehensive understanding of pathogenic fungi is necessary. The present study reports the isolation of a strain of Apiotrichum cacaoliposimilis, which is difficult to detect using conventional clinical assays, from the sterile urine samples of a patient with a urinary tract infection. Sanger sequencing of the internal transcribed spacer regions confirmed the genus of the microbe, while whole-genome sequencing yielded the initial genome assembly of A. cacaoliposimilis. A total of 7,161 predicted proteincoding genes were mapped using multiple databases, including Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, non-redundant protein database, Pathogen-Host Interactions Database, and Comprehensive Antibiotic Resistance Database. The phenotypic data, biochemical reactions, and antimicrobial susceptibility analyses were conducted to reveal the metabolic properties, virulence, and drug resistance profile of the isolated A. cacaoliposimilis. The rank-sum test revealed the differences in the intergeneric distribution of the highly virulent genes UgeB and Pem2. In addition, other genes exhibited significant overlap in terms of virulence factors with the clinical isolate Apiotrichum mycotoxinivorans GMU1709. Fortunately, similar to most fungi belonging to the Apiotrichum genus, the isolate investigated in the present study was also sensitive to the drug voriconazole (MIC = 0.06 μg/ml). In summary, the phylogenetic placement, potential pathogenic genes, drug sensitivity patterns, and morphological characteristics of the isolated A. cacaoliposimilis were determined precisely in the present study.
Pengaruh Kombinasi Bungkil Sawit Terfermentasi Dengan Natura Organik Dekomposer dan Ampas Tahu Terhadap Laju Pertumbuhan Maggot BSF (Hermetia illucens )
Authors: Christiana Ivone , Maryani Maryani , Rozik Mohamad , Matling Matling , Lumbantoruan Yosua 
Published: December 2024

BSF maggots (Hermetia illucens) also have quite high nutritional content, making BSF maggots able to be used as feed for fish. Maggot BSF works to convert organic waste into simpler biomass. Organic waste from agricultural waste is a medium for maggot enlargement that can be used as feed. Agricultural organic waste in Indonesia is available in quite large quantities. The types of waste that can be used as cultivation media are tofu waste and palm oil cake waste.Â This study aims to determine the effect of the combination of fermented palm oil cake with natural organic decomposer and tofu waste on the growth rate of Maggot BSF (Hermetia illucens). The method used in this research is the experimental method using Completely Randomized Design (CRD). The factors studied were the combination of fermented palm oil cake with natural organic decomposer and tofu waste on the growth of Maggot BSF which consisted of 4 treatment levels, namely A (100% fermented palm oil cake); B (75% fermented palm oil + 25% tofu dregs); C (50% fermented palm oil + 50% tofu dregs); D (25% fermented palm oil + 75% tofu waste). Parameters observed were BSF maggot length and weight growth, digested feed conversion efficiency (ECD), waste reduction index (WRI), BSF maggot productivity.Â Data used is Analysis of Variance (ANOVA) and to determine the best treatment using a follow-up test in the form of Duncan's test with a significance level of 5%. The results showed that the combination of fermented palm oil cake with different natural organic decomposers and tofu dregs had a significant effect on the productivity of BSF maggots. The best combination of fermented palm oil cake with natural organic decomposer and tofu waste on the growth rate of Maggot BSF is 100% fermented palm oil cake which has a significant effect when compared to other treatments.
Identifikasi Ektoparasit Pada Ikan Lele Dumbo (Clarias gariepinus) Yang Di Budidayakan di Kolam Beton
Authors: Welnando Rydho , Maryani Maryani , Tantulo Uras , Rozik Mohamad 
Published: December 2024

There are several obstacles for dumbo catfish farmers that result in losses, one of which is disease. Diseases caused by parasitic infections can cause irritation to external organs such as gills and skin. This can cause disruption of respiration and osmoregulation processes and reduce fish immunity. The population of ectoparasites in fish in the aquatic environment can be monitored through the identification of parasites by calculating their prevalence and intensity. This study aims to identify ectoparasites, analyze the prevalence rate, intensity of ectoparasite attacks that attack dumbo catfish. Sampling for 5 times in one week for 20 working days with the number of samples to be obtained as much as the catch of 3 stations. Ectoparasites found in dumbo catfish are Dactylogyrus sp, Trichodina sp, Chilodonella sp, and Oodinium sp. The highest prevalence value was found in Dactylogyrus sp at station III with a percentage of 100% with a very frequent infection category. The highest intensity value was Trichodina sp. at station II which was 24.4 individuals/head with moderate category. The dominating ectoparasite was Dactylogyrus sp. The number of infected dumbo catfish was 30 out of 30 samples tested. In Dactylogyrus sp there were a total of 258 parasites, followed by Chilodonella sp with a total of 69 parasites from 30 samples, Oodinium sp had a total of 47 parasites and Trichodina sp had a total of 151 parasites from 30 samples. Water quality parameters measured during the study showed good values for the growth of dumbo catfish in the rearing ponds.
Intensitas Serangan Jamur Ganoderma sp Pada Perkebunan Kelapa Sawit Lahan Mineral dan Lahan Pasang Surut
Authors: Rahmana Billy Aditia , Hayata Hayata , Hartawan Rudi 
Published: December 2024

Stem rot disease caused by fungus (Ganoderma sp) is a major challenge in oil palm farming. This fungus infects oil palm trees at every stage of growth. This disease has a gradualy development, which the symptoms only appearing in the last stages, so it is called as "silent killer" of oil palm trees. This study aims to determine the attacks intensity of Ganoderma sp fungus on oil palms planted in mineral land and tidal land. This research was conducted on mineral land in Suko Awin Jaya Village, Muaro Jambi Regency and tidal land in Petanang Kumpeh Village. This research was conducted in May - July 2024. The research was conducted under unformatted trial design. The research location was purposive chosen for there were relatively uniform plants in that location, and the Ganoderma sp attacks were found in that place namely:1. Mineral land, 2. Tidal land. There were 2 sampling sites, in two different land, with an area of 1 Ha, which were randomly selected from 5 hectares of existing plants. Census sampling method was used to determination the trees as a sample at the research location so sample totaly were 135 trees. The results of this study showed that on mineral land the attacks percentation was 2.61% and the attacks intensity was 1.99% higher than the attacks percentation and attacks intensity on tidal land representative 1.50% and 1.13%.
Study of Serum Calcium and Magnesium Levels in Reproductive Women Using Oral and Injectable Contraceptives
Authors: Rezwana Karim Roshny  , M A Muttalib  , Gazi Sharmin Sultana  , Farzana Akonzee Mishu  , Md Imran Khan Chayon  
Published: December 2024

Background: The oral and injectable contraceptives fulfill the human need for birth control with great effectiveness. These can effectively prevent pregnancy and alleviate menstrual disorder when used correctly. Many biochemical profiles of women taking oral and injectable contraceptives are disturbed due to metabolic alterations induced by its hormonal content. Objective: To find out the effects of oral and injectable contraceptives on serum calcium and magnesium levels. Materials and Methods: This cross-sectional comparative study was done in BIRDEM General Hospital, Shaheed Suhrawardy Medical College Hospital and Gandaria Family Planning Clinic of Nagar Shastho Kendro from July 2018 to June 2019. Study parameters serum calcium and magnesium were estimated in each case. One hundred fifty women aged 18 to 45 were included according to the inclusion criteria. They were divided into three groups－50 oral contraceptive users as group I, 50 injectable contraceptive users as group II and 50 normal healthy controls as group III. After taking the informed written consent from the study subjects, a structured questionnaire was filled in for each subject to collect data including personal identification, age, sex, chief complaints, duration of contraceptive use, socioeconomic status, demographic history, family history, genetic history, drug history, menstrual history and history of other systemic diseases. Results: The results of the study showed mean age and SD of the study subjects in Group I, Group II and Group III were 31.08±6.67, 31.60±7.83 and 36.74±6.27 years respectively. Mean (±SD) age was significantly higher in control group (Group III) than Group I and Group II (p <0.001). This study found that mean (±SD) serum calcium of the study subjects in Group I, Group II and Group III were 9.34±0.47, 9.20±0.40 and 9.14±0.49 mg/dL (p <0.05). The mean serum calcium was significantly higher in Group I compared to Group II and III. The mean value of magnesium in Group I, II and III were 0.82±0.09, 0.85±0.11 and 0.80±0.09 mmol/L respectively. There was significant increase in serum magnesium in injectable contraceptive users than oral contraceptive users and control group. Conclusion: This study concluded that oral contraceptives increased the serum calcium and injectable contraceptives increased the serum magnesium levels in comparison with control individuals and these were significant.
J Enam Med Col 2022; 12(2): 95−101
Breaking chains across generations: exploring the nexus between intergenerational educational mobility and domestic violence among Indian women
Authors: Choudhary Akanksha , Singh Ashish 
Published: December 2024

IntroductionIn this article, authors have investigated the relationship between intergenerational educational mobility and the chances of experiencing domestic violence among Indian women. This perhaps is the first ever attempt to demonstrate this relationship not just in the Indian context but also in the global scholarship on domestic violence.MethodsThe analysis is based on logistic regression using the data ‘India Youth Survey: Situation and Needs’. Authors have controlled for various individual, familial, and community-level factors in achieving the results.ResultsFindings indicate that women who have more years of education than their mothers have significantly lesser chances of experiencing domestic violence. Furthermore, daughters whose mothers have been victims of domestic violence are highly likely to experience it themselves. Besides, women whose husbands consume alcohol, come from low income strata or live in nuclear families have significantly higher odds of experiencing domestic violence. Also, it was observed that the odds of experiencing domestic violence vary significantly for different castes, regions, religions as well as rural and urban areas. Insights from this study can contribute to policymaking aimed at empowering women through education, especially when their mothers have not had a significant education. Additionally, the study further substantiates the role of factors such as maternal experience of domestic violence, husband’s alcohol consumption, low income levels, and family structure in determining the likelihood of experiencing domestic violence. Therefore, the findings support existing scholarship for designing targeted interventions to address these specific risk factors, ultimately contributing to creating safer environments for women.ConclusionMerely educational attainments do not affect the chances of domestic violence to a large extent. It is probably the confidence a woman derives on account of better educational attainments as compared to her previous generation that influences her take on the menace of domestic violence.
Unveiling neuroimmunology profile of immunological non-responders in HIV: a multimodal MRI approach
Authors: Zhang Yang , Ji Jiahao , Zheng Luyao , Cai Miaotian , Sun Guangqiang , Ma Yundong , Zhang Xin , Chen Xue , Zhang Yulin , Lin Xiao , Li Zhen , Zhang Tong 
Published: December 2024

BackgroundPeople living with HIV (PLWH), especially immunological non-responders (INRs), may experience adverse neurologic events. However, the extent of neurological impairment in INRs remains uncertain. This study evaluates brain structure and function, immune dysregulation, and peripheral immunomarkers in INRs and immunological responders (IRs) among PLWH, classified according to immunological response criteria, within a clinical research setting.MethodsThis study utilized multi-modal MRI to assess brain structure and function in INRs (n = 25) and IRs (n = 53). Mass cytometry and Luminex/ELISA assays were employed to analyze peripheral blood monocytes, T cell subpopulations, cytokines, chemokines, neurotrophic factors, and endocrine factors.ResultsNeuroimaging findings revealed notable changes in brain structure and function in INRs, including increased fractional amplitude of low-frequency fluctuations and regional homogeneity in the left middle temporal gyrus, as well as decreased grey matter volume in the left middle temporal gyrus, left lingual gyrus, and right rolandic operculum. Furthermore, INRs exhibited significant alterations in functional connectivity in the temporal and occipital gyrus. Mass cytometry analysis demonstrated significant immune dysregulation in INRs, characterised by increased levels of senescent and activated T cells, and heightened monocyte activation. Additionally, noteworthy associations were found between neurological abnormalities and peripheral levels of immunomarkers, inflammatory cytokines, chemokines, neurotrophic factors, and endocrine factors in INRs.ConclusionThese findings underscore the associations between immune dysfunction and changes in brain structure and function, emphasizing the importance of further investigations in this field.
Immunogenicity and protective efficacy of recombinant chimeric antigens based on surface proteins of Toxoplasma gondii
Authors: Chyb Maciej , Ferra Bartłomiej Tomasz , Kawka Malwina , Skwarecka Marta , Dziadek Bożena , Gatkowska Justyna 
Published: December 2024

IntroductionToxoplasmosis is caused by the opportunistic, cosmopolitan protozoan Toxoplasma gondii is one of the most common parasitoses in the world. This parasite can pose a threat to people with immunodeficiency but also to the fetus, since the invasion can lead to miscarriages. Moreover, this parasite can contribute to economic losses in livestock farming. These problems lead to the implementation of new, safe solutions for the development of effective toxoplasmosis immunoprophylaxis.MethodsIn this work, newly produced recombinant trivalent chimeric proteins of T. gondii, based on SAG1-SAG2 recombinant chimeric antigen that differ in one terminal antigenic component, were tested in terms of their ability to induce an effective post-vaccination response. Antigens were tested in vitro to assess their ability to elicit APC cells response and further mice of the C3H/HeOuJ strain were immunized using those antigens, to evaluate their immunogenicity and immunoprotective effect in vivo. Two weeks after the last dose mice were either sacrificed to assess selected parameters of the immune response or infected with T. gondii DX strain to determine the degree of protection one month later.ResultsThe results of serological tests revealed a high level of serum IgG antibodies specific for the native T. gondii TLA antigens. TLA-stimulated splenocytes produced cytokines that are important in inhibiting protozoal invasion. Additionally, CD3+ CD4+ and CD3+ CD8+ T cell subpopulations of splenocytes were analysed by flow cytometry. One month after experimental infection mice were sacrificed, and their brains were isolated to count T. gondii tissue cyst. Immunization of mice with recombinant trivalent chimeric proteins of T. gondii resulted in reduction of tissue cyst burden rates reaching even 74%.DiscussionThe obtained results demonstrate strong immunogenicity of the studied proteins and will allow to select candidates for further research aimed at increasing the immunoprotective properties of experimental vaccines against toxoplasmosis based on T. gondii chimeric antigens.
Association between serum calcium level and the risk of acute kidney injury in ICU patients with subarachnoid hemorrhage: a retrospective cohort study
Authors: Zhao Zhenlin , Xiao Kuntai , Zhao Sirong , Liu Kangfeng , Huang Fu , Xiao Hua 
Published: December 2024

AimThis study aimed to evaluate the association between serum calcium level and the risk of acute kidney injury (AKI) in patients with subarachnoid hemorrhage (SAH).MethodsIn this retrospective cohort study, data on adults from the Medical Information Mart for Intensive Care (MIMIC-III and MIMIC-IV) databases, spanning from 2008 to 2019, were extracted. In the logistic regression models, confounding variables, including age, white blood cell (WBC), systolic blood pressure (SBP), heart rate, blood urea nitrogen (BUN), glucose, international normalized ratio (INR), and the Charlson Comorbidity Index (CCI), were finally adjusted by stepwise regression. The outcome event was the occurrence of AKI after intensive care unit (ICU) admission. The univariate and multivariate logistic regression models were utilized to assess the association between serum calcium level and the risk of AKI in SAH patients, with odds ratios (ORs) and 95% confidence intervals (CIs). To further explore the association, subgroup analyses were performed, stratified by age, Glasgow Coma Scale (GCS) scores, drugs, and surgical methods.ResultsA total of 1,128 patients with SAH were included in the study, of which 457 patients developed AKI. Low levels of serum calcium were significantly associated with a high risk of AKI in patients with SAH, with an OR (95%CI) of 1.38 (1.01–1.89). Further subgroup analyses showed that low levels of calcium were significantly associated with a high risk of AKI in SAH patients aged ≥60 years (OR = 0.27, 95%CI: 0.09–0.83), who had GCS score ≥13 (OR = 1.57, 95%CI: 1.08–2.30), who did not use calcium channel blockers (CCB) (OR = 2.22, 95%CI: 1.16–4.25) and angiotensin-converting enzyme (ACE) inhibitors (OR = 1.51, 95%CI: 1.06–2.14), and who did not undergo aneurysm embolization (OR = 1.48, 95%CI: 1.01–2.17) and aneurysm clipping (OR = 1.45, 95%CI: 1.04–2.01).ConclusionThe results of our study indicated that low levels of serum calcium were significantly associated with the risk of AKI in patients with SAH.
Developing a novel dosiomics model to predict treatment failures following lung stereotactic body radiation therapy
Authors: Bhandari Ashok , Johnson Kurtis , Oh Kyuhak , Yu Fang , Huynh Linda M. , Lei Yu , Wisnoskie Sarah , Zhou Sumin , Baine Michael James , Lin Chi , Zhang Chi , Wang Shuo 
Published: December 2024

PurposeThe purpose of this study was to investigate the dosiomics features of the interplay between CT density and dose distribution in lung SBRT plans, and to develop a model to predict treatment failure following lung SBRT treatment.MethodsA retrospective study was conducted involving 179 lung cancer patients treated with SBRT at the University of Nebraska Medical Center (UNMC) between October 2007 and June 2022. Features from the CT image, Biological Effective Dose (BED) and five interaction matrices between CT and BED were extracted using radiomics mathematics. Our in-house feature selection pipeline was utilized to evaluate and rank features based on their importance and redundancy, with only the selected non-redundant features being used for predictive modeling. We randomly selected 151 cases and 28 cases as training and test datasets. Four different models were trained utilizing the Balanced Random Forest framework on the same training dataset to differentiate between failure and non-failure cases. These four models utilized the same number of selected features extracted from CT-only, BED-only, a combination of CT and BED, and a composite of CT and BED including their interaction matrices, respectively.ResultsThe cohort included 125 non-failure cases and 54 failure cases, with a median follow-up time of 34.4 months. We selected the top 17 important and non-redundant features (with the Pearsons’s coefficient &lt; 0.5) in each model. When evaluated on the same independent test set, the four models—CT features-only, BED features-only, a combination of CT and BED features, and a composite model including features from CT and BED that includes their interaction matrices—achieved AUC values of 0.56, 0.75, 0.73, and 0.82, respectively, with corresponding accuracies of 0.61, 0.79, 0.71, and 0.79. The composite model demonstrated the highest AUC and accuracy, indicating that incorporating interactions between CT and BED reveals more predictive capabilities in distinguishing between failure and non-failure cases.ConclusionThe dosiomics model integrating the interaction between CT and Dose can effectively predict treatment failure following lung SBRT treatment and may serve as a useful tool to proactively evaluate and select lung SBRT treatment plans to reduce treatment failure in the future.