bluuebunny/crossref_metadata_2025_split huggingface dataset:
A triple‐band circularly polarized annular ring antenna with asymmetric ground plane for wireless applications
Authors: Dhara Reshmi , Mitra Monojit 
Published: March 2020

AbstractHerein a compact triple‐band circularly polarized (CP) microstrip patch antenna, loaded with a single annular ring patch with eight symmetrical slots along its periphery and a series of octagonal closed ring resonators (CRRs) on the opposite side of the substrate, is proposed. Evolution process of the antenna geometry reveals that the eight symmetrical slots and the CRRs are responsible for increased impedance bandwidth (IBW), while simulated (high‐frequency structure simulator (HFSS) and computer simulation tool) triple CP bands obtained due to the uses of crossshaped slit resonating at 7.5 GHz (152.2 MHz); asymmetric ground plane at 10.2 GHz (161 MHz); and square open‐loop at 12.2 GHz (112.5 MHz). Series of parasitic strips improved CP radiation and remove ripple in the radiation pattern. The antenna presented here, is compact, fabricated with an optimized dimension of 35 × 30 × 1.6 mm3, on low cost FR‐4 substrate with copper cladding. The measured <10‐dB IBW ranges from 4.08‐5.05 GHz and 5.98 GHz up to 13.6 GHz. Simulated maximum peak gain found to be 4.45 dBi at 13 GHz. The antenna proposed herein is appropriate for wireless applications.
Boosting oxygen evolution reaction by activation of lattice‐oxygen sites in layered Ruddlesden‐Popper oxide
Authors: Zhu Yinlong , Tahini Hassan A. , Hu Zhiwei , Yin Yichun , Lin Qian , Sun Hainan , Zhong Yijun , Chen Yubo , Zhang Feifei , Lin Hong‐Ji , Chen Chien‐Te , Zhou Wei , Zhang Xiwang , Smith Sean C. , Shao Zongping , Wang Huanting 
Published: March 2020

AbstractEmerging anionic redox chemistry presents new opportunities for enhancing oxygen evolution reaction (OER) activity considering that lattice‐oxygen oxidation mechanism (LOM) could bypass thermodynamic limitation of conventional metal‐ion participation mechanism. Thus, finding an effective method to activate lattice‐oxygen in metal oxides is highly attractive for designing efficient OER electrocatalysts. Here, we discover that the lattice‐oxygen sites in Ruddlesden‐Popper (RP) crystal structure can be activated, leading to a new class of extremely active OER catalyst. As a proof‐of‐concept, the RP Sr3(Co0.8Fe0.1Nb0.1)2O7‐δ (RP‐SCFN) oxide exhibits outstanding OER activity (eg, 334 mV at 10 mA cm−2 in 0.1 M KOH), which is significantly higher than that of the simple SrCo0.8Fe0.1Nb0.1O3‐δ perovskite and benchmark RuO2. Combined density functional theory and X‐ray absorption spectroscopy studies demonstrate that RP‐SCFN follows the LOM under OER condition, and the activated lattice oxygen sites triggered by high covalency of metal‐oxygen bonds are the origin of the high catalytic activity.image
A gas outburst prevention and control strategy for single thick coal seams with high outburst risk: A case study of Hudi Coal Mine in Qinshui Basin
Authors: Li Hong , Ma Jinkui , Wang Zhiquan , Wang Wei , Liu Yanwei 
Published: March 2020

AbstractCoal and gas outburst is one of the most harmful natural disaster in coal mine production. The frequency and scope of outburst are closely related to the metamorphic degree of coal seam, the depth of coal seam, and the complexity of geological structure. In general, the use of traditional along‐seam borehole or cross‐seam borehole can meet the needs of gas control. However, for the coal seams with high outburst risk, these methods fail to meet the demands of high‐yield and high‐efficiency coal recovery as well as safe, green, and economical coal mining. Although pressure‐relief gas extraction in the protective seam and coalbed methane (CBM) pre‐extraction can eliminate outburst risk in coal seams, these methods can hardly help realize the green development trend of coal and CBM co‐extraction because they cannot guarantee the current situation of highly mechanized mining. In this study, a three‐dimensional coal and CBM co‐extraction strategy was proposed. The strategy includes three levels of three‐dimensional gas prevention and control methods. The first level is the combination of ground and underground gas extraction methods, to achieve joint extraction of ground and underground gas. The second level is the full‐coverage extraction of the coal resources to be mined, to achieve joint extraction through upper and lower cross‐seam borehole and along‐seam borehole. The third level is the optimization of ground and underground extraction and utilization systems, to realize the full utilization of CBM and to achieve the goal of coal and CBM co‐extraction. The three‐dimensional gas prevention and control method constructs a new gas control model for single thick coal seams with high gas outburst risk and realizes the all‐round coordination and unity of different gas control methods spatially and temporally. The gas control strategy completely eliminates the high gas outburst risk of a single thick coal seam and has been successfully applied in Hudi Coal Mine. The application results verify that the method can effectively guarantee the operation safety during highly mechanized mining, realize extraction and full utilization of separate‐source gas, and provide guidance and direction for coal and CBM co‐extraction with similar extraction conditions.
Rapid and sensitive detection of mycotoxins by advanced and emerging analytical methods: A review
Authors: Singh Jyoti , Mehta Alka 
Published: March 2020

AbstractQuantification of mycotoxins in foodstuffs is extremely difficult as a limited amount of toxins are known to be presented in the food samples. Mycotoxins are secondary toxic metabolites, made primarily by fungal species, contaminating feeds and foods. Due to the presence in globally used grains, it is an unpreventable problem that causes various acute and chronic impacts on human and animal health. Over the previous few years, however, progress has been made in mycotoxin analysis studies. Easier techniques of sample cleanup and advanced chromatographic approaches have been developed, primarily high‐performance liquid chromatography. Few extremely sophisticated and adaptable tools such as high‐resolution mass spectrometry and gas chromatography–tandem MS/MS have become more important. In addition, Immunoassay, Advanced quantitative techniques are now globally accepted for mycotoxin analysis. Thus, this review summarizes these traditional and highly advance methods and their characteristics for evaluating mycotoxins.
Immuno‐oncology gene expression profiling of formalin‐fixed and paraffin‐embedded clear cell renal cell carcinoma: Performance comparison of theNanoString nCountertechnology with targetedRNAsequencing
Authors: Talla Suranand B. , Rempel Eugen , Endris Volker , Jenzer Maximilian , Allgäuer Michael , Schwab Constantin , Kazdal Daniel , Stögbauer Fabian , Volckmar Anna‐Lena , Kocsmar Ildiko , Neumann Olaf , Schirmacher Peter , Zschäbitz Stefanie , Duensing Stefan , Budczies Jan , Stenzinger Albrecht , Kirchner Martina 
Published: March 2020

AbstractInflammatory gene signatures are currently being explored as predictive biomarkers for immune checkpoint blockade, and particularly for the treatment of renal cell cancers. From a diagnostic point of view, the nCounter analysis platform and targeted RNA sequencing are emerging alternatives to microarrays and comprehensive transcriptome sequencing in assessing formalin‐fixed and paraffin‐embedded (FFPE) cancer samples. So far, no systematic study has analyzed and compared the technical performance metrics of these two approaches. Filling this gap, we performed a head‐to‐head comparison of two commercially available immune gene expression assays, using clear cell renal cell cancer FFPE specimens. We compared the nCounter system that utilizes a direct hybridization technology without amplification with an NGS assay that is based on targeted RNA‐sequencing with preamplification. We found that both platforms displayed high technical reproducibility and accuracy (Pearson coefficient: ≥0.96, concordance correlation coefficient [CCC]: ≥0.93). A density plot for normalized expression of shared genes on both platforms showed a comparable bi‐modal distribution and dynamic range. RNA‐Seq demonstrated relatively larger signaling intensity whereas the nCounter system displayed higher inter‐sample variability. Estimated fold changes for all shared genes showed high correlation (Spearman coefficient: 0.73). This agreement is even better when only significantly differentially expressed genes were compared. Composite gene expression profiles, such as an interferon gamma (IFNg) signature, can be reliably inferred by both assays. In summary, our study demonstrates that focused transcript read‐outs can reliably be achieved by both technologies and that both approaches achieve comparable results despite their intrinsic technical differences.
The failure of microglia to digest developmental apoptotic cells contributes to the pathology of RNASET2‐deficient leukoencephalopathy
Authors: Hamilton Noémie , Rutherford Holly A. , Petts Jessica J. , Isles Hannah M. , Weber Thomas , Henneke Marco , Gärtner Jutta , Dunning Mark J. , Renshaw Stephen A. 
Published: March 2020

AbstractThe contribution of microglia in neurological disorders is emerging as a leading disease driver rather than a consequence of pathology. RNAseT2‐deficient leukoencephalopathy is a severe childhood white matter disorder affecting patients in their first year of life and mimicking a cytomegalovirus brain infection. The early onset and resemblance of the symptoms to a viral infection suggest an inflammatory and embryonic origin of the pathology. There are no treatments available for this disease as our understanding of the cellular drivers of the pathology are still unknown. In this study, using a zebrafish mutant for the orthologousrnaset2gene, we have identified an inflammatory signature in early development and an antiviral immune response in mature adult brains. Using the optical transparency and the ex utero development of the zebrafish larvae we studied immune cell behavior during brain development and identified abnormal microglia as an early marker of pathology. Live imaging and electron microscopy identified that mutant microglia displayed an engorged morphology and were filled with undigested apoptotic cells and undigested substrate. Using microglia‐specific depletion and rescue experiments, we identified microglia as drivers of this embryonic phenotype and potential key cellular player in the pathology of RNAseT2‐deficient leukoencephalopathy. Our zebrafish model also presented with reduced survival and locomotor defects, therefore recapitulating many aspects of the human disease. Our study therefore placed ourrnaset2mutant at the forefront of leukodystrophy preclinical models and highlighted tissue‐specific approaches as future therapeutic avenues.
Biological tumor markers (maspin, CD105, nm23‐H1) and disease relapse in laryngeal cancer: cluster analysis
Authors: Franz Leonardo , Tealdo Giulia , Contro Giacomo , Bandolin Luigia , Carraro Valentina , Giacomelli Luciano , Alessandrini Lara , Blandamura Stella , Marioni Gino 
Published: March 2020

AbstractBackgroundThe aim of this investigation was to see if a panel of biomarkers (maspin, CD105, and nm23‐H1) could be used to stratify patients with laryngeal squamous cell carcinoma (LSCC) in homogeneous disease recurrence risk clusters.MethodsCluster analysis was used to classify 89 patients based on their immunohistochemical expression of nm23‐H1, CD105, and maspin.ResultsOur analysis identified seven homogeneous clusters: the LSCC recurrence rate was lowest in cluster 6 (non‐nuclear maspin pattern, nuclear nm23‐H1 expression ≥10%, endothelial CD105 expression <6%; P = .009), and highest in cluster 3 (non‐nuclear maspin pattern, nuclear nm23‐H1 expression <10%, endothelial CD105 expression ≥6%; P <.001).ConclusionsSimilar panels of biological variables identified by cluster analysis should be tested in prospective clinical trials to establish whether treating patients identified as being at higher risk of LSCC recurrence more aggressively could significantly improve their recurrence rate and/or disease‐specific survival.
A Randomized Double-Blind Placebo-Controlled First-In-Human Phase 1 Trial of TransCon PTH in Healthy Adults
Authors: Karpf David B , Pihl Susanne , Mourya Sanchita , Mortensen Eva , Kovoor Eshwari , Markova Denka , Leff Jonathan A 
Published: March 2020

ABSTRACT
TransCon PTH is a sustained-release, essentially inactive prodrug transiently bound to an inert carrier, designed to release PTH(1-34), and in development for hypoparathyroidism (HP). This phase 1, randomized, placebo-controlled, single and multiple ascending dose (SAD and MAD, respectively) trial evaluated safety, tolerability, pharmacodynamics (PD), and pharmacokinetics (PK) of TransCon PTH in healthy adults. SAD and MAD cohorts consisted of 10 subjects (eight active, two placebo) who received up to seven single or six multiple ascending doses of TransCon PTH, respectively. TransCon PTH doses ranged from 3.5 to 124 μg PTH(1-34) for the SAD cohorts and 3.5 to 24 μg PTH(1-34)/day for the MAD cohorts. The primary PK endpoint was Free PTH. The PD endpoints included albumin adjusted serum calcium (sCa), fractional excretion of calcium (FECa), intact endogenous PTH(1-84), bone turnover markers, renal tubular maximum reabsorption of phosphate/glomerular filtration rate (TMP/GFR), serum phosphate (sP) and magnesium, and 1,25 dihydroxyvitamin D. TransCon PTH was generally well tolerated; there were no drug-related serious adverse events (SAEs), and all AEs were transient in nature. Free PTH demonstrated an effective half-life of approximately 60 hours and a dose-dependent, sustained exposure with an infusion-like profile within the calculated physiologic range for active PTH at steady-state. Albumin-adjusted sCa demonstrated a dose-dependent, sustained response with complete control of FECa despite modest hypercalcemia at higher doses. Renal tubular maximum reabsorption of phosphate/glomerular filtration rate (TMP/GFR) showed a dose-dependent decrease, resulting in a dose-dependent decrease in sP. TransCon PTH administered daily for 10 days showed no increase in the osteoblastic bone formation markers, serum bone-specific alkaline phosphatase (BSAP) or P1NP, or the osteoclastic bone resorption marker, urine NTx, but modestly and transiently increased the osteoclast marker, serum CTx. These phase 1 data support TransCon PTH as a daily replacement therapy for HP providing physiological levels of PTH 24 hours per day and advancement into phase 2 clinical development. © 2020 The Authors. Journal of Bone and Mineral Research published by American Society for Bone and Mineral Research.
Gastrointestinal Tolerability and Absorption ofR‐VersusR,S‐Lipoic Acid in Progressive Multiple Sclerosis: A Randomized Crossover Trial
Authors: Cameron Michelle , Taylor Cassidy , Lapidus Jodi , Ramsey Katrina , Koop Dennis , Spain Rebecca 
Published: March 2020

AbstractWe compared the gastrointestinal (GI) tolerability and assessed for bioequivalent absorption ofR‐lipoic acid (LA) in people with progressive multiple sclerosis (MS) in a single‐center, double‐blind, randomized crossover trial. Participants randomly assigned to formulation sequence took 600 mg ofR‐LA or 1200 mg of a 1:1 racemicR,S‐LA mixture in single daily doses for 7 to 10 days, underwent a washout of at least 7 days, and then took the other form of LA for 7 to 10 days. At the end of each period on LA, GI symptoms were assessed with GI questions from the Monitoring of Side Effects Scale. Serum LA concentrations were measured before and 60, 90, 120, 180, and 240 minutes after the first and last day's dose of each form of LA to derive an area under the plasma concentration–time curve (AUC) and maximum serum concentration (Cmax). Twenty participants enrolled (12 women; 15 secondary progressive MS, 5 primary progressive MS; mean age, 59.6 years). Two withdrew early due to symptoms while takingR,S‐LA, and one withdrew early while takingR‐LA. The mean GI Monitoring of Side Effects Scale score was 1.7 points lower onR‐LA than onR,S‐LA (P= .069), and there were fewer reports of each GI side effect when taking theR‐LA than theR,S‐LA (31 vs 60;P= .025). The AUC and CmaxforR‐LA were bioequivalent for the 2 formulations (90% confidence intervals 97.4% to 99.3% for AUC and 93.4% to 98.2% for Cmax). This study supports that in people with progressive MS, there is better GI tolerability and bioequivalent serum absorption ofR‐LA when 600 mg ofR‐LA is taken asR‐LA alone than when taken in a 1:1 racemicR,S‐LA mixture.
Addressing the spatial disconnect between national‐scale total maximum daily loads and localized land management decisions
Authors: Amin M. G. Mostofa , Veith Tamie L. , Shortle James S. , Karsten Heather D. , Kleinman Peter J. A. 
Published: March 2020

AbstractRegulatory watershed mitigation programs typically emphasize widespread adoption of best management practices (BMPs) to meet total maximum daily load (TMDL) goals. To comply with the Chesapeake Bay TMDL, jurisdictions must develop watershed implementation plans (WIPs) to determine the number and type of BMPs to implement. However, the spatial resolution of the bay‐level model used to determine these load reduction goals is so coarse that the regulatory plan cannot consider heterogeneity in local conditions, which affects BMP effectiveness. Using the Topo‐SWAT modification of the Soil and Water Assessment Tool (SWAT), we simulated two BMP adoption scenarios in the Spring Creek watershed in central Pennsylvania to determine if leveraging fine‐scale spatial heterogeneity to place BMPs could achieve the same (or better) nutrient and sediment reduction at a lower cost than the state‐level WIP BMP adoption recommendations. Topo‐SWAT was initialized with detailed land use and management practice information, systematically calibrated, and validated against 12 yr of observed data. After determining individual BMP cost effectiveness, results were ranked to design a cost‐effective BMP adoption scenario that achieved equal or greater load reduction as the WIP scenario for 74% of the cost using eight management‐based BMPs: no‐till, manure injection, cover cropping, riparian buffers, land retirement, manure application timing, wetland restoration, and nitrogen management (15% less N input). Because watersheds of this size typically represent the smallest modeling unit in the Chesapeake Bay Model, results demonstrate the potential to use watershed models with finer inference scales to improve recommendations for BMP implementation under the Chesapeake Bay TMDL.
Environmentally friendly and efficient method for the synthesis of the new α,α′‐diimine ligands with benzimidazole moiety
Authors: Mamedov Vakhid A. , Zhukova Nataliya A. , Kadyrova Milyausha S. , Fazleeva Rezeda R. , Bazanova Olga B. , Beschastnova Tat'yana N. , Gubaidullin Aidar T. , Rizvanov Il'dar Kh. , Yanilkin Vitaliy V. , Latypov Shamil K. , Sinyashin Oleg G. 
Published: March 2020

AbstractThe new α,α′‐diimine ligands with benzimidazole moiety were synthesized based on the rearrangement of 3‐aroylquinoxalin‐2(1H)‐ones when exposed to 4,5‐diamino‐2,1,3‐benzoxadiazole, 4,5‐diamino‐2,1,3‐benzothiadiazole and 5,6‐diaminoquinoxaline. Among them, we report the first examples of the new heterocyclic system namely benzo[4′,5′]imidazo[1′,2′:1,2]quinolino[3,4‐b and 4,3‐b][1,2,5]oxadiazolo[3,4‐f]quinoxalines, which exhibits an interesting electrochemical behavior. All compounds were fully characterized by IR, 1H and 13C NMR spectroscopies, and mass spectrometry.
Conditioning adhesive contacts between the neutrophils and the endotheliocytes by Staphylococcus aureus
Authors: Pleskova Svetlana N. , Kriukov Ruslan N. , Bobyk Sergey Z. , Boryakov Alexey V. , Gorelkin Peter V. , Erofeev Alexander S. 
Published: March 2020

AbstractWe have developed a model for evaluating the integral intercellular interactions in the “endotheliocyte‐neutrophil” system and have shown the high variability of adhesion contacts in different donors associated with different expression profiles of neutrophils. Two methods (forсe spectroscopy‐spectroscopy and scanning ion‐conductance microscopy) showed a decrease in the rigidity of the membrane‐cytoskeletal complex of neutrophils under the influence of Staphylococcus aureus 2879 M. Adding this strain to the “endotheliocyte‐neutrophil” system caused a statistically significant decrease in the adhesion force and adhesion work, which indicates a change in the expression profile and physicochemical properties of membranes of both types of interacting cells (neutrophils and endotheliocytes).
Potential of proteomics to probe microbes
Authors: Shiny Matilda Chellaiah , Madhusudan Iyengar , Gaurav Isola Ravi , Shanthi Chittibabu 
Published: March 2020

AbstractAn organism exposed to a plethora of environmental perturbations undergoes proteomic changes which enable the characterization of total proteins in it. Much of the proteomic information is obtained from genomic data. Additional information on the proteome such as posttranslational modifications, protein–protein interactions, protein localization, metabolic pathways, and so on are deduced using proteomic tools which genomics and transcriptomics fail to offer. The proteomic analysis allows identification of precise changes in proteins, which in turn solve the complexity of microbial population providing insights into the microbial metabolism, cellular pathways, and behavior of microorganisms in new environments. Furthermore, they provide clues for the exploitation of their special features for biotechnological applications. Numerous techniques for the analysis of microbial proteome such as electrophoretic, chromatographic, mass spectrometric‐based methods as well as quantitative proteomics are available which facilitate protein separation, expression, identification, and quantification of proteins. An understanding of the potential of each of the proteomic tools has created a significant impact on diverse microbiological aspects and the same has been discussed in this review.
Dummy template surface molecularly imprinted polymers based on silica gel for removing imidacloprid and acetamiprid in tea polyphenols
Authors: Chen Jingyu , Huang Xin , Wang Li , Ma Chaoyang , Wu Shijia , Wang Hongxin 
Published: March 2020

AbstractDummy template surface molecularly imprinted polymers based on silica gel were prepared through the surface molecular imprinting technique. Nonpoisonous nicotinamide, which is a structural analogue of imidacloprid and acetamidine, was chosen as the dummy template molecule. The obtained polymers were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, and X‐ray diffraction. The results showed that the polymers exhibited high adsorption capacity and selectivity for imidacloprid and acetamiprid. The maximum adsorption capacities of the polymers toward imidacloprid and acetamiprid were 42.05 and 22.99 mg/g, and the adsorption could reach binding equilibrium within 150 min. The polymers were successfully applied as column‐filling materials to extract imidacloprid and acetamiprid from tea polyphenols with a relatively high removal rate (92.36 and 95.20%). The polymers also showed great stability and reusability during the application. The obtained polymers possessed good application prospects for removing imidacloprid and acetamiprid in tea polyphenol production processes.