bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protection against a chlamydial respiratory challenge by a chimeric vaccine formulated with the Chlamydia muridarum major outer membrane protein variable domains using the Neisseria lactamica porin B as a scaffold
Authors: Tifrea Delia F. , Pal Sukumar , Fairman Jeff , Massari Paola , de la Maza Luis M. 
Published: May 2020

AbstractChlamydia trachomatisis the most frequently detected sexually transmitted bacterial pathogen in the world. Attempts to control these infections with screening programs and antibiotics have failed and, therefore, a vaccine is the best approach to control this epidemic. TheChlamydiamajor outer membrane protein (MOMP) is the most protective subunit vaccine so far tested. Protection induced by MOMP is, in part, dependent on its tertiary structure. We have previously described new recombinant antigens composed of theNeisseria lactamicaPorB engineered to express the variable domains (VD) fromChlamydia muridarumMOMP. Here we tested antigens containing each individual MOMP VD and different VD combinations. Following immunization, mice were challenged intranasally withC. muridarum. Our results show that three constructs, PorB/VD1–3, PorB/VD1–4, and PorB/VD1–2–4, elicited high serum IgG titers in vivo, significant IFN-γ levels upon T cells re-stimulation in vitro, and evidence of protective immunity in vivo. PorB/VD1–3, PorB/VD1–4, and PorB/VD1–2–4 immunized mice lost less body weight, had lighter lungs, and decreased numbers of inclusion forming units (IFUs) in lungs than other PorB/VD construct tested and mock PBS-immunized mice. These results suggest that this approach may be a promising alternative to the use of MOMP in aChlamydiavaccine.
Assessment of Genetic Heritability in Rice Breeding Lines Based on Morphological Traits and Caryopsis Ultrastructure
Authors: Roy Subhas Chandra , Shil Pankaj 
Published: May 2020

AbstractRice (Oryza sativa L) is a most important staple food crop of the world because more than half of the World’s population is dependent on it for their livelihood. Global rice production must be doubled by 2050 to cope up with the situation of population growth. Narrow genetic base in the released varieties has made the improvement in plateaus. Widening the genetic base is necessary to overcome the yield barrier. Hybridization and pre-breeding has been carried out to broaden the genetic base. Heritability and genetic advances were measured in the F5 lines (Tulaipanji × IR64), F3 lines (Tulaipanji × IR64 × PB1460), and F3 lines (Badshabhog × Swarna sub1). Some of the breeding lines were showing promising field performance with high yield potentiality. Wide crosses were performed to widen the genetic base between (Ranjit × O. rufipogon) and (Badshabhog × O. rufipogon) and the heritability pattern of the morphological characteristics in the progeny lines was evaluated. Nutritional quality of the rice grain is totally dependent on the morphology and histological characteristics of the caryopsis which are genetically determined. Caryopses ultrastructural analyses were carried out in seventeen different rice breeding lines through SEM. SEM analysis showed distinguishing ultrastructure in respect to pericarp, testa, aleurone layer, protein bodies and starchy endosperm in the breeding lines with distinctive inheritance pattern. This study provides information about the cross compatibility of the wide hybridization and heritability measures of the morphological traits which may supplement the breeding program to break the yield plateaus.
The Volumetric Source Function: Looking Inside van der Waals Interactions
Authors: Tantardini Christian , Michalchuk Adam A. L. , Samtsevich Artem , Rota Carlo , Kvashnin Alexander G. 
Published: May 2020

AbstractThe study of van der Waals interactions plays a central role in the understanding of bonding across a range of biological, chemical and physical phenomena. The presence of van der Waals interactions can be identified through analysis of the reduced density gradient, a fundamental parameter at the core of Density Functional Theory. An extension of Bader’s Quantum Theory of Atoms in Molecules is developed here through combination with the analysis of the reduced density gradient. Through this development, a new quantum chemical topological tool is presented: the volumetric source function. This technique allows insight into the atomic composition of van der Waals interactions, offering the first route towards applying the highly successful source function to these disperse interactions. A new algorithm has been implemented in the open-source code, CRITIC2, and tested on acetone, adipic and maleic acids molecular crystals, each stabilized by van der Waals interactions. This novel technique for studying van der Waals interactions at an atomic level offers unprecedented opportunities in the fundamental study of intermolecular interactions and molecular design for crystal engineering, drug design and bio-macromolecular processes.
Soil invertebrate diversity loss and functional changes in temperate forest soils replaced by exotic pine plantations
Authors: Cifuentes-Croquevielle Camila , Stanton Daniel E. , Armesto Juan J. 
Published: May 2020

AbstractThe global expansion of tree plantations is often claimed to have positive effects for mitigating global warming, preventing soil erosion, and reducing biodiversity loss. However, questions remain unanswered about the impacts of plantations on belowground diversity and soil properties. Here, we examine how forestry plantations of exotic trees affect critical soil functions and the composition of invertebrate assemblages, by comparing invertebrate diversity and soil physico-chemical properties between non-native Pinus radiata plantations, and nearby native forests in a region of extensive plantation activity in south-central Chile. We quantified differences in diversity, abundance, and community composition of soil invertebrates, as well as fundamental soil properties such as soil water content, water infiltration, nutrient status, and pH. We show that in this landscape mosaic of native forest and plantations, both soil invertebrate communities and physical soil properties differed significantly between systems, despite similar soil origins and topographies. We found a significant loss of soil carbon and a major reduction in taxonomic and functional diversity of soil invertebrates in pine plantation sites. Soil biotic and abiotic characteristics of plantations differed significantly from native forests in plantation-dominated south-central Chile, with profound consequences for ecosystem processes and resilience to future climate change.
Orthorhombic crystal structure and oxygen deficient cluster distribution model for YBa2Cu3−xAlxO6+δ superconductor
Authors: Manju P. , Rajak Neeraj K. , Alex Andrews P. , Kamble Vinayak B. , Jaiswal-Nagar D. 
Published: May 2020

AbstractSingle crystal x-ray diffraction measurements on both as-grown as well as oxygenated single crystals of an aluminium doped high temperature superconductor YBa2Cu3−xAlxO6+δ revealed the crystal structure to be orthorhombic with space group Pmmm, in contrast to, tetragonal crystal structures corresponding to space group P4/mmm, previously reported for as-grown YBa2Cu3−xAlxO6+δ, and conflicting structures on oxygenated YBa2Cu3−xAlxO6+δ. The orthorhombic crystal structure was confirmed by powder x-ray diffraction that showed the presence of two peaks corresponding to (020) and (200) reflections associated with orthorhombic structures of space group Pmmm, instead of a single (200) reflection corresponding to tetragonal crystal structures with space group P4/mmm. All the as-grown crystals were found to be superconducting. An oxygen-vacancy cluster distribution model is proposed to explain the differences in the obtained magnetisation hysteresis loop and the broad superconducting transition temperature. The model proposes the existence of two oxygen deficient clusters of (Al-..-Cu-O-Cu)n and (Cu-O-Cu-..)n juxtaposed with each other whose number and size vary as the as-grown single crystals of YBa2Cu3−xAlxO6+δ are subjected to oxygenation. X-ray photoelectron spectroscopy measurements showed the existence of two distinct peaks in each of the spectrum of O, Cu, Y and Ba in YBa2Cu3−xAlxO6+δ crystals corresponding to the two different types of clusters. The relative intensities of each XPS peak was found to decrease in the oxygenated crystals as compared to the as-grown ones confirming the change in the number and size of clusters in the as-grown crystals after oxygenation.
Randomized controlled trial of medium cut-off versus high-flux dialyzers on quality of life outcomes in maintenance hemodialysis patients
Authors: Lim Jeong-Hoon , Park Yeongwoo , Yook Ju-Min , Choi Soon-Youn , Jung Hee-Yeon , Choi Ji-Young , Park Sun-Hee , Kim Chan-Duck , Kim Yong-Lim , Cho Jang-Hee 
Published: May 2020

AbstractMedium cut-off (MCO) dialyzers help remove larger middle molecules associated with symptoms related to the accumulation of uremic retention solutes. We investigated the effect of an MCO dialyzer on the improvement of quality of life (QOL) in maintenance hemodialysis (HD) patients. Forty-nine HD patients with high-flux dialysis were randomly assigned to either an MCO (Theranova 400, Baxter) or a high-flux (FX CorDiax 80 or 60, Fresenius Medical Care) dialyzer and completed the study. QOL was assessed at baseline and after 12 weeks of treatment using the Kidney Disease Quality of Life Short Form-36, and pruritus was assessed using a questionnaire and visual analog scale. The reduction ratios of middle molecules were also evaluated. Laboratory markers, including serum albumin, did not differ between the two groups after 12 weeks. Removals of kappa and lambda free light chains were greater for MCO dialyzer than high-flux dialyzer. The MCO group had higher scores than the high-flux group in the domains of physical functioning and physical role (75.2 ± 20.8 vs. 59.8 ± 30.1, P = 0.042; 61.5 ± 37.6 vs. 39.0 ± 39.6, P = 0.047, respectively), and the MCO group had lower mean scores for morning pruritus distribution and the frequency of scratching during sleep (1.29 ± 0.46 vs. 1.64 ± 0.64, P = 0.034; 0.25 ± 0.53 vs. 1.00 ± 1.47, P = 0.023, respectively). MCO dialyzers may improve patient-reported outcomes, particularly the physical components of QOL and uremic pruritus, in patients with high-flux dialyzers.
Risk of Serious Falls Between Hemodialysis and Peritoneal Dialysis Patients: A Nationwide Population-based Cohort Study
Authors: Wang Hsi-Hao , Wu Jia-Ling , Lee Yi-Che , Ho Li-Chun , Chang Min-Yu , Liou Hung-Hsiang , Hung Shih-Yuan 
Published: May 2020

AbstractThe association between serious falls and dialysis modality [hemodialysis (HD) and peritoneal dialysis (PD)] is unclear. A nationwide population-based retrospective cohort study with 127,823 end-stage renal disease patients aged over 18 years was conducted with the unmatched cohort of 101,304 HD and 7,584 PD patients retrieved from Taiwan’s National Health Insurance Research Database during 2000–2013. A total of 7,584 HD and 7,584 PD patients matched at 1:1 ratio by propensity score were enrolled to the study. Serious falls were defined by the diagnostic codes, E code, and image studies. Cox regression model and competing-risk model were used for statistical analysis. HD patients were older and had more comorbidities at baseline than PD patients. After matching and adjustment, HD patients had a higher risk of serious falls than PD patients [sHR 1.27 (95% CI 1.06–1.52)]. Females, elders, a history of falls before dialysis, comorbidity with stroke or visual problems, using diuretics, α-blockers, and mydriatics were associated with higher risks of serious falls among dialysis patients. The risk of serious falls was higher in HD patients than PD patients. Health professionals should create age-friendly environments, reduce unnecessary medications, and raise patients’ awareness of falls in daily life.
Synergistic effect of graphene oxide and zoledronic acid for osteoporosis and cancer treatment
Authors: Boran Gökçen , Tavakoli Sepideh , Dierking Ingo , Kamali Ali Reza , Ege Duygu 
Published: May 2020

AbstractZoledronic acid (ZOL) is a third generation bisphosphonate which can be used as a drug for the treatment of osteoporosis and metastasis. In this study, graphene oxide (GO) is conjugated with ZOL, and the nanostructured material is evaluated in terms viability, proliferation and differentiation. Furthermore, the associated morphological changes of bone marrow-derived mesenchymal stem cells (BM-MSC), and Michigan Cancer Foundation-7 (MCF-7) breast cancer cells, as well as the effect of the drugs on mineralization of BM-MSCs are investigated using a variety of characterization techniques including Fourier Transform Infrared Spectroscopy (FTIR), scanning electron microscopy (SEM) as well as alamar blue, acridine orange, and alizarin red assays. Nanostructured ZOL-GO with an optimum performance is synthesized using ZOL and GO suspensions with the concentration of 50 µM and 2.91 ng/ml, respectively. ZOL-GO nanostructures can facilitate the mineralization of BM-MSC cells, demonstrated by the formation of clusters around the cells. The results obtained confirm the performance of ZOL-GO nanostructures as promising drug complexes for the treatment of osteoporosis and metastasis.
Functional and anatomical changes in diabetic macular edema after hemodialysis initiation: One-year follow-up multicenter study
Authors: Takamura Yoshihiro , Matsumura Takehiro , Ohkoshi Kishiko , Takei Tatsuhiko , Ishikawa Kunihiro , Shimura Masahiko , Ueda Tetsuo , Sugimoto Masahiko , Hirano Takao , Takayama Kei , Gozawa Makoto , Yamada Yutaka , Morioka Masakazu , Iwano Masayuki , Inatani Masaru 
Published: May 2020

AbstractDiabetic nephropathy and retinopathy (DR) including diabetic macular edema (DME) are representative microvascular complications of diabetes. We conducted a retrospective multicenter study analyzing records from patients with DR (132 eyes in 70 patients) and end-stage renal diseases (ESRD) who underwent hemodialysis for the first time. We demonstrated that the central retinal thickness (CRT) values were significantly decreased (p < 0.0001), and the best-corrected visual acuity (BCVA) values were improved (p < 0.05) at 1, 3, 6, 9, and 12 months after hemodialysis initiation, in spite of a lack of specific ocular treatments for DME in 93.2% of eyes. We found a significant positive correlation in the rates of CRT changes between right and left eyes. The CRT reductions were greater in eyes with DME type subretinal detachment than in those with spongelike swelling and cystoid macular edema. The visual outcome gain was associated with the CRT reduction at 12 months in the eyes with good initial BCVA (≧20/50). Hemodialysis induction contributed to functional and anatomical improvements after 1 year, independently of initial laboratory values before the hemodialysis.
Potency of Oral Rehydration Solution in Inducing Fluid Absorption is Related to Glucose Concentration
Authors: Buccigrossi Vittoria , Lo Vecchio Andrea , Bruzzese Eugenia , Russo Carla , Marano Antonella , Terranova Sara , Cioffi Valentina , Guarino Alfredo 
Published: May 2020

AbstractOral rehydration solutions (ORSs) is the key treatment of acute diarrhea in children, as it restores the electrolyte balance by stimulating the intestinal sodium/glucose transporter SGLT1 to induce fluid absorption. The World Health Organization (WHO) and The European Society for Paediatric Gastroenterology Hepatology and Nutrition (ESPGHAN) proposed ORSs with different chemical compositions. The main agent of childhood acute gastroenteritis is rotavirus (RV). We evaluate the effects of ORS with different concentration of glucose and sodium on RV induced secretion. Ussing chambers technique was used for electophysiology experiments to evaluate ion fluid flux. ESPGHAN ORS (sodium 60 mmol/L and glucose 111 mmol/L) induced a more potent proabsorptive effect in Caco-2 cells than WHO ORS, and this effect depended on the sodium/glucose ratio. Titration experiments showed that RV-induced fluid secretion can be reverted to a proabsorptive direction when sodium and glucose concentration fall in specific ranges, specifically 45–60 mEq/L and 80–110 mM respectively. The results were confirmed by testing commercial ORSs. These findings indicated that ORS proabsorptive potency depends on sodium and glucose concentrations. Optimal ORS composition should be tailored to reduce RV-induced ion secretion by also considering palatability. These in vitro data should be confirmed by clinical trials.
Convolutional neural networks explain tuning properties of anterior, but not middle, face-processing areas in macaque inferotemporal cortex
Authors: Raman Rajani , Hosoya Haruo 
Published: May 2020

AbstractRecent computational studies have emphasized layer-wise quantitative similarity between convolutional neural networks (CNNs) and the primate visual ventral stream. However, whether such similarity holds for the face-selective areas, a subsystem of the higher visual cortex, is not clear. Here, we extensively investigate whether CNNs exhibit tuning properties as previously observed in different macaque face areas. While simulating four past experiments on a variety of CNN models, we sought for the model layer that quantitatively matches the multiple tuning properties of each face area. Our results show that higher model layers explain reasonably well the properties of anterior areas, while no layer simultaneously explains the properties of middle areas, consistently across the model variation. Thus, some similarity may exist between CNNs and the primate face-processing system in the near-goal representation, but much less clearly in the intermediate stages, thus requiring alternative modeling such as non-layer-wise correspondence or different computational principles.
Mimicking bio-mechanical principles in photonic metamaterials for giant broadband nonlinearity
Authors: Chang Taeyong , Jeon Suwan , Heo Minsung , Shin Jonghwa 
Published: May 2020

AbstractMicroscopic structuring can change the effective properties of a material by several orders of magnitude. An example of this is animal bone, which has an effective elastic modulus that is more than 1,000 times larger than that of the constituent proteins. Here, we propose a broadband-enhancement principle of photonic nonlinearity that has a similar mathematical origin as the bone example. The proposed staggered array metamaterials violate the standard Miller’s rule in nonlinear optics and can enhance the third-order nonlinearity by more than a thousand to a billion times, depending on target operation frequencies. This metamaterial principle also enables manipulation of the individual components of the linear and nonlinear susceptibility tensors. Our biomimetic approach overcomes the fundamental speed-efficiency trade-off in current resonant enhancement schemes, making faster and more efficient all-optical devices possible for 1.55 μm wavelength. The principle is also applicable to ionic diffusion, heat conduction, or other transport problems.
Novel dc flashover model for predicting flashover voltage of the iced insulators
Authors: Fan Caijin , Jiang Xingliang , Xie Yanbin , Hu Jianlin , Zhang Zhijin , Hu Qin , Bi Maoqiang 
Published: May 2020

AbstractThis work first proposes a novel flashover model based on the deduced residual resistance of the ice layer. The most important finding of the field icing tests is that the double‐layer ice‐melting water film exists on the inner and outer surfaces of the ice layer. The conductivity of melting water on the inner and outer surface of the ice layer has been measured, and the corresponding surface conductivity has been calculated. According to the proposed flashover model, the ratio η of the resistance of the ice layer to that of the pollution layer gradually increases with the increase of the ratio k of the resistance of the outer surfaces to that of inner surfaces, and eventually approaches saturation. Furthermore, the icing flashover criterion has been modified, and the voltage gradient of the inner surface is less than that of the outer surface. Test results show that the ice flashover voltage is lower than the pollution flashover voltage between 2.18–9.69% and 14.75–20.32% under light ice and semi‐cylindrical ice, respectively. The simulation results based on the proposed model and the classic model are compared with the field test data. Results show that the proposed model could attain a high accuracy of dc flashover voltage and demonstrate the effectiveness of the proposed model.
Rezidivraten und Form des Rezidivs nach endoluminaler Ablation der Vena saphena magna – Ein Vergleich von Laserablation (EVLA) 1470  nm, Radiofrequenz (RFITT und ClosureFast) sowie Heißdampf mit einer durchschnittlichen Nachbeobachtungszeit von 3,8 Jahren
Authors: Schuler Lisa , Weingard I. , Kiderlen M. , Theodoridis A. , Kriechenbauer N. , Hartmann K. 
Published: May 2020

Zusammenfassung
Einleitung Erkrankungen des Venensystems, insbesondere auch die Insuffizienz der Vena saphena magna (VSM), gehören zu den häufigsten Krankheitsbildern in unserer Gesellschaft. Zur Behandlung stehen neben Krossektomie und Stripping sowie reiner Sklerosierungstherapie verschiedene endovenöse thermische Verfahren zur Verfügung, die in der vorliegenden Studie untereinander verglichen werden sollten.
Methoden Zwischen 2009 und 2013 wurde bei insgesamt 297 Patienten die insuffiziente VSM mit einer der 4 Methoden EVLA 1470 nm, ClosureFast, RFITT oder Heißdampf abladiert. Als primärer Endpunkt wurde die Rezidiv-Häufigkeit definiert. Die Nachkontrollen mit Duplexsonografie fanden 14 Tage postoperativ, 3 Monate postoperativ, nach 1 Jahr und schließlich jährlich mit einer durchschnittlichen Nachbeobachtungszeit von 3,8 Jahren und einer Follow-up-Rate von 81 % statt.
Ergebnisse Zum Zeitpunkt der letzten Nachkontrolle zeigten sich folgende anatomische Erfolgsraten der behandelten VSM: ClosureFast 95 %, EVLA 97 %, RFITT 79 % und Heißdampf 71 %. Schwerwiegende Komplikationen traten nur beim Heißdampf auf (Nekrosen an der Punktionsstelle), die Schmerzintensität lag bei allen Methoden 14 Tage postoperativ bei einem Median von 1–3 auf einer Skala von 1–10, bei den nachfolgenden Kontrollen lag der Median bei allen Methoden bei 1. Sowohl der Global Index Score (CIVIQ-Score) als auch der Venous Clinical Severity Score (VCSS) ließen sich durch alle endovenösen thermischen Methoden signifikant verbessern. In 5–12 % der Fälle trat ein Reflux der zuvor suffizienten Vena saphena magna accessoria (VSAA) auf.
Diskussion EVLA und ClosureFast sind zur Behandlung von Insuffizienzen der VSM mit hoher Erfolgsrate und vergleichbaren Ergebnissen zu Krossektomie und Stripping geeignet. Die Verfahren RFITT und Heißdampf zeigen im Vergleich signifikant niedrigere Verschlussraten und sind damit besonderen Situationen vorbehalten. Besonderes Augenmerk sollte auf das Vorhandensein einer zunächst nicht refluxiven VSAA gerichtet werden. Da sich hier eine erhöhte Rezidivrate bei dieser Vene zeigte, erscheint es sinnvoll, die VSAA primär mit zu abladieren.
Pseudomonas Aeruginosa Sepsis in a Previously Healthy Infant with Subcutaneous Nodules and Mastoid Bone Destruction
Authors: Azapağası Ebru , Öz Fatma Nur , Uysal Yazıcı Mutlu , Ceylan Dilara , Ocak Emre , Taşçı Yıldız Yasemin , Aytekin Caner 
Published: May 2020

Abstract
Pseudomonas aeruginosa septicemia is rare in previously healthy children. Skin lesions such as subcutaneous nodules and ecthyma gangrenosum may be the first manifestation of Pseudomonas infection that have rarely been reported. Herein we reported a previously healthy 6-month-old boy patient who presented with suppurative otitis media, multiple nodules, septic shock, and P. aeruginosa was identified in cultures of the blood, skin lesions, and purulent material of his ears.