bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of bacteriocin-encoding genes of lactic acid bacteria isolated from traditional dairy products of Luxor province, Egypt
Authors: Refay Rasha Mohamed , Abushady Hala Mohamed , Amer Sara Adel , Mailam Mahmoud Ahmed 
Published: June 2020

Abstract
Background
Researchers have focused on isolating and identifying the bacteriocin producing lactic acid bacteria from various food systems especially dairy products. Molecular techniques have been recently used for rabid identification of bacteriocins rather than time-consuming biochemical characters. Global climate disturbances can affect the diversity of beneficial microorganisms in dairy and their products, especially lactic acid bacteria, so it is worth to evaluate their bacteriocinogenicity in different climates. Thus, the aim of this study was to screen for predominant bacteriocin producing lactic acid bacteria (LAB) in traditional dairy products of Luxor governorate at Upper Egypt and determine their bacteriocin-encoding genes.

Results
Eighty-six strains of the LAB were isolated from raw milk and traditional dairy product of Luxor province, Egypt, in which 76.1% and 23.9% were identified as lactic acid bacilli and cocci, respectively. On the basis of their antibacterial potentials, 30 out of 68 LAB isolates were found to be antimicrobial producers. These isolates exhibited a potential antibacterial activity against Salmonella paratyphi B, Escherichia coli, Staphylococcus aureus, and Proteus mirabilis, except for Listeria monocytogenes. LAB isolates were analyzed using species-specific PCR; results emphasized that 22 of isolates were identified as Lactobacillus plantarum, while 8 were Leuconostoc mesenteroides. According to the sequencing of isolates, two strains named Lactobacillus plantarum Egypt 2018 (accession no. MH817034) and Leuconostoc mesenteroides Egypt 2018 (accession no. MH817035) were identified. Detection of bacteriocin-encoding genes was performed by polymerase chain reaction (PCR). The results emphasized that almost all tested Lb. plantarum strains (n = 10) possess both plnA and plnEF genes, whereas the gene encoding mesentericin Y105 was detected in one Lc. mesenteroides of the examined isolates.

Conclusions
This study was effective for the rapid detection of bacteriocin producing strains within dairy products. Extracted bacteriocin could be a valuable source of natural food biopreservative.
Evaluation of the matrix influence on the microscopic pore-throat structures of deep-water tight sandstone: A case study from the Upper Triassic Chang 6 oil group of the Yanchang Formation in the Huaqing area, Ordos Basin, China
Authors: Chen Zhaobing , Zhou Chuangfei , Chen Xinjing , Zhu Yujie , Zhu Yushuang 
Published: June 2020

Former studies have suggested that the matrix of clastic rocks is unfavorable for the storage-percolation of reservoirs. However, the contribution of the matrix to the microscopic pore-throat structures in deep-water tight sandstone cannot be ignored. Aiming at the deep-water tight sandstone of the Chang 6 reservoir in the Ordos Basin (China), we have evaluated the characteristics of the matrix and the secondary pores in the matrix based on a multiscale microscopic identification and testing method, to reveal the influence of the matrix on the types, distribution, and heterogeneity of the pore throats. The results show that, unlike cements, the composition of the matrix is complex, characterized by its poor crystal form with no cement generation relationship. The structure of the matrix components is not completely dense. Intercrystalline pores and dissolved matrix pores are developed in the matrix after diagenetic modification, with a pore diameter of 20–1000 nm. These pores form a complex matrix secondary pore network. The matrix controls the number and volume of 0–1 μm pore throats. The matrix is constructive to the distribution of pore throats when its content is ≤7%. This positive effect gradually decreases with the increase of the matrix content, intensifying the compaction of the reservoir. The matrix controls the heterogeneities of the pore throat structures in deep-water tight sandstone. Large pore throats are gradually separated and disintegrated into a large number of micro-/nanopores by the matrix with the increase of the matrix content. Meanwhile, the fractal dimensions approached 3, increasing the complexity of the pore structures. Therefore, the matrix is favorable and unfavorable to the microscopic pore throat structures of the reservoir. The matrix not only results in the loss of intergranular pores but also generates a large number of secondary micro-/nanopore throat networks with complex structures, constituting an effective space for hydrocarbon accumulation and percolation in deep-water tight sandstone.
The lateral plate mesoderm
Authors: Prummel Karin D. , Nieuwenhuize Susan , Mosimann Christian 
Published: June 2020

ABSTRACTThe lateral plate mesoderm (LPM) forms the progenitor cells that constitute the heart and cardiovascular system, blood, kidneys, smooth muscle lineage and limb skeleton in the developing vertebrate embryo. Despite this central role in development and evolution, the LPM remains challenging to study and to delineate, owing to its lineage complexity and lack of a concise genetic definition. Here, we outline the processes that govern LPM specification, organization, its cell fates and the inferred evolutionary trajectories of LPM-derived tissues. Finally, we discuss the development of seemingly disparate organ systems that share a common LPM origin.
MAIN INDICATORS OF SURGICAL PROFILE BED FUND OF THE ROUND-THE-CLOCK AND DAILY STAY HOSPITALS IN THE RUSSIAN FEDERATION AND ITS REGIONS
Authors: Mel'nikov Yuriy , Bant'eva M. , Manoshkina E. , Kuznecova V. 
Published: June 2020

From 2010 to 2018 the level of hospitalization in surgical beds in round-the-clock hospitals decreased (by 19.9%), while in the daily hospitals of medical organizations providing inpatient care increased (by 36.4%). At the same time, the level of hospitalization in daily stay hospitals of medical organizations providing outpatient care in 2014 - 2108 virtually unchanged (0.2% increase).
Thus, it is possible to conclude, that the hospital-replacing function has realized in the country and there has been a redistribution of the volume of surgical care from round-the-clock hospital beds to daily stay hospital beds, however, the bed fund of both round-the-clock and daily stay is characterized by extreme disproportionality in different regions of the Russian Federation, what may indicate an imbalance in structural and functional transformations that should be carried out taking into account the characteristics of each particular region.
CHEMICAL COMPOSITION OF ORGANIC SUBSTANCE OF OAK BAR ORDINARY (EMERGENCY) (QUERCUS ROBUR L, BEECH FAMILY - FAGACCAC) (MESSAGE III - CHLOROFORM EXTRACT)
Authors: Platonov V. , Hadarcev Aleksandr , Suhih G. , Frankevich V. , Volochaeva M. , Dunaev V. 
Published: June 2020

This report presents the results of chromatography-mass spectrometry of a chloroform extract obtained after preliminary sequential exhaustive extraction of the bark of ordinary oak (petiole) n-hexane and toluene. The purpose of the study is to significantly expand the range of compounds that determine the composition of the organic matter of the bark of ordinary oak (petiole). Materials and research methods. The dried raw material is ground to a powder in a porcelain laboratory mill, which is then subjected to exhaustive sequential extraction with n-hexane and toluene at boiling points. Then, the solid residue was dried to constant weight in a vacuum oven, and then extraction was carried out with chloroform at its boiling point. Extraction with chloroform was completed when the refractive index was reached equal to its initial value, after which chloroform was distilled off in a vacuum rotary evaporator to obtain a dark brown oily residue.
Thus, we can conclude that the pharmacological effect of the chloroform extract of ordinary oak bark (petiolate) will be determined by the presence of significant amounts of sterols, hydrocarbons, which are dominated by arenes, alkenes and cycloalkanes, esters formed mainly by dicarboxylic acid (Oxalic acid).
The Causal Effect of Service Satisfaction on Customer Loyalty
Authors: Huang Guofang , Sudhir K. 
Published: June 2020

We propose an instrumental-variable (IV) approach to estimate the causal effect of service satisfaction on customer loyalty by exploiting a common source of randomness in the assignment of service employees to customers in service queues. Our approach can be applied at no incremental cost by using routine repeated cross-sectional customer survey data collected by firms. The IV approach addresses multiple sources of biases that pose challenges in estimating the causal effect using cross-sectional data: (1) the upward bias from common-methods variance resulting from the joint measurement of service satisfaction and loyalty intent in surveys, (2) the attenuation bias caused by measurement errors in service satisfaction, and (3) the omitted variable bias that may be in either direction. In contrast to the common concern about the upward common-methods bias in estimates using cross-sectional survey data, we find that ordinary-least-squares substantially underestimates the causal effect, suggesting that the downward bias resulting from measurement errors and/or omitted variables is dominant. The underestimation is even more significant with a behavioral measure of loyalty, where there is no common-methods bias. This downward bias leads to significant underestimation of the positive profit impact from improving service satisfaction and can lead to underinvestment by firms in service satisfaction. Finally, we find that the causal effect of service satisfaction on loyalty is greater for more difficult types of services. This paper was accepted by Juanjuan Zhang, marketing.
PRECONCEITO OU PRÉ-CONCEITO? CONSTRUINDO SENTIDOS SOBRE PRECONCEITO E SAÚDE À LUZ DA HERMENÊUTICA DE HANS-GEORG GADAMER: UMA REVISÃO INTEGRATIVA.
Authors: Deluque Júnior Romano , Costa Márcio Luís 
Published: June 2020

Objetivou-se trazer um panorama à respeito do tema do preconceito dentro da perspectiva teórica da Hermenêutica Filosófica tal como é proposta por Hans-Georg Gadamer. Procedeu-se à uma revisão de literatura na modalidade de uma Revisão Integrativa que perguntou pelos principais entendimentos acerca do tema do preconceito trazidos pelos trabalhos publicados nos últimos 10 anos. 17 estudos foram selecionados e categorizados seguindo tais categorias: ano de publicação, país de publicação, país de realização da pesquisa, periódico, área temática, ramo de atuação dos autores, indicadores de preconceito, delineamentos metodológicos, ferramenta para análise de dados, características das amostras, procedimentos éticos, e principais resultados e conclusões. A discussão tratou das problemáticas que mais emergiram dentro dos trabalhos selecionados. Concluiu-se ser, a hermenêutica filosófica, uma eficiente ferramenta para a construção de uma visão humanizada de saúde dentro de uma perspectiva onde todo conhecimento é antecedido por um preconceito que o antecede.
Accurate Nonendoscopic Detection of Barrett's Esophagus by Methylated DNA Markers: A Multisite Case Control Study
Authors: Iyer Prasad G. , Taylor William R. , Johnson Michele L. , Lansing Ramona L. , Maixner Kristyn A. , Hemminger Lois L. , Cayer Frances K. , Yab Tracy C. , Devens Mary E. , Slettedahl Seth W. , Broderick Brendan T. , Mahoney Douglas W. , McGlinch Maria C. , Berger Calise K. , Foote Patrick H. , Giakomopoulos Maria , Allawi Hatim , Smyrk Thomas C. , Wang Kenneth K. , Katzka David A. , Wolfsen Herbert C. , Burke James A. , Ahlquist David A. , Kisiel John B. 
Published: June 2020

INTRODUCTION:
Nonendoscopic Barrett's esophagus (BE) screening may help improve esophageal adenocarcinoma outcomes. We previously demonstrated promising accuracy of methylated DNA markers (MDMs) for the nonendoscopic diagnosis of BE using samples obtained from a capsule sponge-on-string (SOS) device. We aimed to assess the accuracy of these MDMs in an independent cohort using a commercial grade assay.

METHODS:
BE cases had ≥ 1 cm of circumferential BE with intestinal metaplasia; controls had no endoscopic evidence of BE. The SOS device was withdrawn 8 minutes after swallowing, followed by endoscopy (the criterion standard). Highest performing MDMs from a previous study were blindly assessed on extracted bisulfite-converted DNA by target enrichment long-probe quantitative amplified signal (TELQAS) assays. Optimal MDM combinations were selected and analyzed using random forest modeling with in silico cross-validation.

RESULTS:
Of 295 patients consented, 268 (91%) swallowed the SOS device; 112 cases and 89 controls met the pre-established inclusion criteria. The median BE length was 6 cm (interquartile range 4–9), and 50% had no dysplasia. The cross-validated sensitivity and specificity of a 5 MDM random forest model were 92% (95% confidence interval 85%–96%) and 94% (95% confidence interval 87%–98%), respectively. Model performance was not affected by age, gender, or smoking history but was influenced by the BE segment length. SOS administration was well tolerated (median [interquartile range] tolerability 2 [0, 4] on 10 scale grading), and 95% preferred SOS over endoscopy.

DISCUSSION:
Using a minimally invasive molecular approach, MDMs assayed from SOS samples show promise as a safe and accurate nonendoscopic test for BE prediction.
Association of Digestive Symptoms and Hospitalization in Patients With SARS-CoV-2 Infection
Authors: Cholankeril George , Podboy Alexander , Aivaliotis Vasiliki Irene , Pham Edward A. , Spencer Sean P. , Kim Donghee , Ahmed Aijaz 
Published: June 2020

INTRODUCTION:
High rates of concurrent gastrointestinal manifestations have been noted in patients with corona virus disease 2019 (COVID-19); however, the association between these digestive manifestations and need for hospitalization has not been established.

METHODS:
This is a retrospective review of consecutive patients diagnosed with COVID-19. A total of 207 patients were identified; 34.5% of patients noted concurrent gastrointestinal symptoms, with 90% of gastrointestinal symptoms being mild.

RESULTS:
In a multivariate regression model controlled for demographics and disease severity, an increased risk of hospitalization was noted in patients with any digestive symptom (adjusted odds ratio 4.84, 95% confidence interval: 1.68–13.94).

DISCUSSION:
The presence of digestive symptoms in COVID-19 is associated with a need for hospitalization.
Characterization of a Knock-In Mouse Line Expressing a Fusion Protein of κ Opioid Receptor Conjugated with tdTomato: 3-Dimensional Brain Imaging via CLARITY
Authors: Chen Chongguang , Willhouse Alex H. , Huang Peng , Ko Nora , Wang Yujun , Xu Bin , Huang Lan Hsuan Melody , Kieffer Brigitte , Barbe Mary F. , Liu-Chen Lee-Yuan 
Published: June 2020

AbstractActivation of κ opioid receptor (KOR) produces analgesia, antipruritic effect, sedation and dysphoria. To characterize neuroanatomy of KOR at high resolutions and circumvent issues of specificity of KOR antibodies, we generated a knock-in mouse line expressing KOR fused at the C terminus with the fluorescent protein tdTomato (KtdT). The selective KOR agonist U50,488H caused anti-scratch effect and hypolocomotion, indicating intact KOR neuronal circuitries. Clearing of brains with CLARITY revealed three-dimensional (3-D) images of distribution of KOR, and any G-protein-coupled receptors, for the first time. 3-D brain images of KtdT and immunohistochemistry (IHC) on brain sections with antibodies against tdTomato show similar distribution to that of autoradiography of [3H]U69,593 binding to KOR in wild-type mice. KtdT was observed in regions involved in reward and aversion, pain modulation, and neuroendocrine regulation. KOR is present in several areas with unknown roles, including the claustrum (CLA), dorsal endopiriform nucleus, paraventricular nucleus of the thalamus (PVT), lateral habenula (LHb), and substantia nigra pars reticulata (SNr), which are discussed. Prominent KtdT-containing fibers were observed to project from caudate putamen (CP) and nucleus accumbens (ACB) to substantia innominata (SI) and SNr. Double IHC revealed co-localization of KtdT with tyrosine hydroxylase (TH) in brain regions, including CP, ACB, and ventral tegmental area (VTA). KOR was visualized at the cellular level, such as co-localization with TH and agonist-induced KOR translocation into intracellular space in some VTA neurons. These mice thus represent a powerful and heretofore unparalleled tool for neuroanatomy of KOR at both the 3-D and cellular levels.
Metabolic syndrome detection with biomarkers in childhood cancer survivors
Authors: Pluimakers V G , van Waas M , Looman C W N , de Maat M P , de Jonge R , Delhanty P , Huisman M , Mattace-Raso F U S , van den Heuvel-Eibrink M M , Neggers S J C M M 
Published: June 2020

Purpose:
Augmented survival of childhood nephroblastoma and neuroblastoma has increased long-term side effects such as metabolic syndrome (MetS). Risk stratification is difficult after abdominal radiation because waist circumference underestimates adiposity. We aimed to develop a strategy for determining MetS in irradiated survivors using an integrated biomarker profile and vascular ultrasonography.

Methods:
The NCEP-ATPIII MetS-components, 14 additional serum biomarkers and 9 vascular measurements were assessed in a single-centre cohort of childhood nephroblastoma (n = 67) and neuroblastoma (n = 36) survivors and controls (n = 61). Multivariable regression models were used to study treatment effects. Principal component analysis (PCA) was used to study all biomarkers in a combined analysis, to identify patterns and correlations.

Results:
After 27.5 years of follow-up, MetS occurred more often in survivors (14%) than controls (3%). Abdominal radiotherapy and nephrectomy, to a lesser extent, were associated with MetS and separate components and with several biomarker abnormalities. PCA of biomarkers revealed a pattern on PC1 from favourable lipid markers (HDL-cholesterol, adiponectin) towards unfavourable markers (triglycerides, LDL-cholesterol, apoB, uric acid). Abdominal radiotherapy was associated with the unfavourable biomarker profile (β = 1.45, P = 0.001). Vascular measurements were not of added diagnostic value.

Conclusions:
Long-term childhood nephro- and neuroblastoma survivors frequently develop MetS. Additional assessment of biomarkers identified in PCA – adiponectin, LDL, apoB, and uric acid – may be used especially in abdominally irradiated survivors, to classify MetS as alternative for waist circumference. Vascular ultrasonography was not of added value.
Vitamin D and PTH: data from a cross-sectional study in an equatorial population
Authors: Neves Marques de Queiroz Natércia , Trindade Cunha de Melo Franciane , de Souza Resende Fabrício , Corrêa Janaú Luísa , Jorge Kzan de Souza Neto Norberto , Nascimento de Lemos Manuela , Lobato Virgolino Ana Carolina , Neres Iunes de Oliveira Maria Clara , Leite de Alcântara Angélica , Vilhena de Moraes Lorena , Franco David Tiago , Maia da Silva Wanderson , Souza Reis Scarlatt , Costa dos Santos Márcia , Contente Braga de Souza Ana Carolina , Freire Piani Pedro Paulo , Arroyo Lara Mourão Neyla , Mileo Felício Karem , Felício Abrahão Neto João , Felício João Soares 
Published: June 2020

Objective:
Investigate the prevalence of vitamin D deficiency in an equatorial population through a large-sample study.

Methods:
Cross-sectional study with 30,224 healthy individuals from the North Region, in Brazil (Amazônia – state of Pará), who had 25-hydroxy-vitamin D (25(OH)D) and intact parathyroid hormone (PTH) serum levels measured by immunoassay method. Those with history of acute or chronic diseases were excluded. Abnormal levels of calcium, creatinine, glycemia and albumin were also exclusion criteria.

Results:
25(OH)D levels were 29.1 ± 8.2 ng/mL and values <12.7 ng/mL were equal to < −2 s.d. below average. Hypovitaminosis D was present in 10% of subjects according to the Institute of Medicine (values <20 ng/mL) and in 59%, in consonance with Endocrine Society (values 20–30 ng/mL as insufficiency and <20 ng/mL as deficiency) criteria. Individuals were divided according to four age brackets: children, adolescents, adults and elderly, and their 25(OH)D levels were: 33 ± 9; 28.5 ± 7.4; 28.3 ± 7.7; 29.3 ± 8.5 ng/mL, respectively. All groups differed in 25(OH)D, except adolescents vs adults. Regression model showed BMI, sex, living zone (urban or rural) and age as independent variables to 25(OH)D levels. Comparing subjects with vitamin D deficiency (<20 ng/mL) to those with vitamin D insufficiency (20–30 ng/mL), a difference between PTH levels in these two groups was observed (95.9 ± 24.7 pg/mL vs 44.2 ± 64.5 pg/mL; P < 0.01). Additionally, the most accurate predictive vitamin D level for subclinical hyperparathyroidism in ROC curve was 26 ng/mL.

Conclusion:
Our equatorial population showed low prevalence of vitamin D hypovitaminosis ranging with age bracket. The insufficient category by Endocrine Society was corroborated by our PTH data.
Circulating plasma proteins and new-onset diabetes in a population-based study: proteomic and genomic insights from the STANISLAS cohort
Authors: Ferreira João Pedro , Lamiral Zohra , Xhaard Constance , Duarte Kévin , Bresso Emmanuel , Devignes Marie-Dominique , Le Floch Edith , Roulland Claire Dandine , Deleuze Jean-François , Wagner Sandra , Guerci Bruno , Girerd Nicolas , Zannad Faiez , Boivin Jean-Marc , Rossignol Patrick 
Published: June 2020

Objective:
Determining the factors associated with new-onset pre-diabetes and type 2 diabetes mellitus (T2D) is important for improving the current prevention strategies and for a better understanding of the disease.

Design:
To study the factors (clinical, circulating protein and genetic) associated with new onset pre-diabetes and T2D in an initially healthy (without diabetes) populational familial cohort with a long follow-up (STANISLAS cohort).

Methods:
A total of 1506 participants attended both the visit 1 and visit 4, separated by ≈20 years. Over 400 proteins, GWAS and genetic associations were studied using models adjusted for potential confounders. Both prospective (V1 to V4) and cross-sectional (V4) analyses were performed.

Results:
People who developed pre-diabetes (n = 555) and/or T2D (n = 73) were older, had higher BMI, blood pressure, glucose, LDL cholesterol, and lower eGFR. After multivariable selection, PAPP-A (pappalysin-1) was the only circulating protein associated with the onset of both pre-diabetes and T2D with associations persisting at visit 4 (i.e. ≈20 years later). FGF-21 (fibroblast growth factor 21) was a strong prognosticator for incident T2D in the longitudinal analysis, but not in the cross-sectional analysis. The heritability of the circulating PAPP-A was estimated at 44%. In GWAS analysis, the SNP rs634737 was associated with PAPP-A both at V1 and V4. External replication also showed lower levels of PAPP-A in patients with T2D.

Conclusions:
The risk of developing pre-diabetes and T2D increases with age and with features of the metabolic syndrome. Circulating PAPP-A, which has an important genetic component, was associated with both the development and presence of pre-diabetes and T2D.