bluuebunny/crossref_metadata_2025_split huggingface dataset:
NLRP3 inflammasome and endoplasmic reticulum stress in the epileptogenic zone in temporal lobe epilepsy: molecular insights into their interdependence
Authors: Yue J. , Wei Y. J. , Yang X. L. , Liu S. Y. , Yang H. , Zhang C. ‐Q. 
Published: April 2020

AimNod‐like receptor protein 3 (NLRP3) inflammasome‐mediated inflammation has emerged as a contributor to epileptogenesis. Endoplasmic reticulum stress (ERS) plays an important role in epilepsy‐induced neurodegeneration. NLRP3 activation and ERS reactions share the same induction factors, suggesting that these processes may be interdependent. However, the correlation between NLRP3 and ERS in TLE has not been confirmed.MethodsThe expression patterns of NLRP3 inflammasome and ERS‐related markers in the temporal neocortices of TLE patients were investigated by western blotting, immunohistochemistry and immunofluorescent labelling. Correlations between the protein levels of NLRP3 and the expression of ERS‐related markers were assessed using Spearman’s rank correlation test. To observe the relationship between the NLRP3 inflammasome and ERS, inhibitors were used in a status epilepticus (SE) model.ResultsOur results show that NLRP3 inflammasome components and ERS‐related markers were upregulated in the temporal neocortices of TLE patients, and were mainly localized to neurons, astrocytes and microglia. We found a positive correlation between the protein levels of NLRP3 and the expression of ERS‐related markers in the temporal neocortices of 20 TLE patients. Furthermore, after blocking the NLRP3 inflammasome with MCC950, the expression of ERS‐related markers was markedly decreased in the hippocampi of SE mice. Moreover, TUDCA, a specific ERS inhibitor, also reduced the expression of NLRP3 components in the hippocampus under SE conditions.ConclusionTaken together, our data reveal the interdependence of the NLRP3 inflammasome and ERS in the epileptogenic zone of TLE patients and in the hippocampi of mice in the early post‐SE phase.
Efficacy of Buzzy® on pain and anxiety during catheterization in children
Authors: Yılmaz Dilek , Özyazıcıoğlu Nurcan , Çıtak Tunç Gülseren , Aydın Ayla İrem , Atak Meryem , Duygulu Şengül , Demirtaş Zeliha 
Published: April 2020

AbstractBackgroundThe use of a peripheral intravenous cannula is a common clinical practice, and it is known to be a major source of pain and anxiety in children. The aim of this study was to examine the effect of the use of the Buzzy® on pain and anxiety in children during peripheral cannula application.MethodsThe research sample consisted of 60 children between the ages of 8 and 16. For children in the experimental group, external cold and vibration were applied by means of the Buzzy® device. Before and during the peripheral intravenous cannula procedure, the levels of fear and anxiety relating to the procedure of the child patients in both the experimental and control groups were assessed by the children themselves and by an independent observer. Immediately after the vein entry procedure had been carried out, the level of pain felt by the children was determined.ResultsThe results of the statistical analysis showed no statistically significant difference between the anxiety levels of the groups before and after the procedure (P > 0.05). The results of the statistical analysis also showed no statistically significant difference between the postprocedural mean pain scores of the children as reported by the children themselves and by the observer (P > 0.05).ConclusionsIn this study, unlike most studies in the literature, the conclusion was reached that the use of the Buzzy® to reduce pain and anxiety during the application of a peripheral intravenous cannula in children was not effective.
The nitrification process for nitrogen removal in biofloc system aquaculture
Authors: Robles‐Porchas Glen Ricardo , Gollas‐Galván Teresa , Martínez‐Porchas Marcel , Martínez‐Cordova Luis Rafael , Miranda‐Baeza Anselmo , Vargas‐Albores Francisco 
Published: April 2020

AbstractAquaculture is an economic activity that faces the unavoidable problem of water quality detriment, which is mainly generated by the improper management of production ponds, including inadequate water circulation and aeration, accumulation of undigested food residues, excretion of metabolic by‐products by the cultivated organisms and other. In addition, the increase in suspended organic matter together with the presence and generation of nitrogen compounds can severely affect the physiology of the animal, leading to significant losses in production. Ammonia (NH3), nitrite (NO2) and nitrate (NO3) are toxic in different scales and environmental conditions; therefore, reducing their concentrations in the culture units has paramount relevance. In this regard, bioflocs are an efficient alternative to transform nitrogen compounds into a non‐toxic form, taking advantage of the capabilities of the microbial communities conforming them. Also, nitrogen can be harnessed and incorporated as organic nitrogen, making the biofloc a source of useful natural food for the cultivated organism. The substantial reduction in the rate of water exchange favoured by the biofloc technology (BFT) is a beneficial advantage for both the production systems and the environment, diminishing the risk of introducing pathogens into the pond in parallel with the improvement in the water quality of effluents. Ammonia oxidation is an advantage adding value to the BFT systems and is discussed in this review.
Insights into Flood Risk Misperceptions of Homeowners in the Dutch River Delta
Authors: Mol Jantsje M. , Botzen W. J. Wouter , Blasch Julia E. , de Moel Hans 
Published: April 2020

AbstractFlooding is one of the most significant natural disasters worldwide. Nevertheless, voluntary take‐up of individual damage reduction measures is low. A potential explanation is that flood risk perceptions of individual homeowners are below objective estimates of flood risk, which may imply that they underestimate the flood risk and the damage that can be avoided by damage reduction measures. The aim of this article is to assess possible flood risk misperceptions of floodplain residents in the Netherlands, and to offer insights into factors that are related with under‐ or overestimation of perceived flood risk. We analyzed survey data of 1,848 homeowners in the Dutch river delta and examine how perceptions of flood probability and damage relate to objective risk assessments, such as safety standards of dikes, as well as heuristics, including the availability heuristic and the affect heuristic. Results show that many Dutch floodplain inhabitants significantly overestimate the probability, but underestimate the maximum expected water level of a flood. We further observe that many respondents apply the availability heuristic.
In vitro elution of amikacin and Dispersin B from a polymer hydrogel
Authors: Hagen Chris R. M. , Singh Ameet , Weese J. Scott , Marshall Quinn , zur Linden Alex , Gibson Thomas W. G. 
Published: April 2020

AbstractObjectiveTo characterize the in vitro elution of amikacin and Dispersin B (β‐N‐acetylglucosaminidase) in a degradable hydrogel.Study designIn vitro, prospective study.MethodsAmikacin (group A; 40 mg/mL), Dispersin B (group D; 70 μg/mL), or combined amikacin and Dispersin B (group AD; 40 mg/mL and 70 μg/mL, respectively) were added to a hydrogel. Ten aliquots per group were incubated in phosphate‐buffered saline that was exchanged at 1, 4, 8, 12, and 24 hours and then once daily for 10 days. Eluted amikacin and Dispersin B were quantitated by using an amikacin reagent kit and a Dispersin B enzyme‐linked immunosorbent assay kit, respectively. Time point drug concentrations were compared between groups by using repeated‐measures analysis of variance, and total drug elution was compared by using an area under the curve calculation.ResultsAmikacin alone, Dispersin B alone, and amikacin and Dispersin B combined together underwent rapid elution in the first 24 hours, followed by a gradual decrease over 10 days. The concentration of Dispersin B eluted in group D was higher at 1 day and lower from day 5 to day 10 compared with that in group AD. The concentration of amikacin eluted in group A was higher at 1, 4, and 8 hours and on day 10 and lower on day 1 compared with that in group AD. The total elution of amikacin was greater from group AD compared with that from group A (P = .02).ConclusionCombining amikacin and Dispersin B had an affect on the total elution of amikacin but not Dispersin B.Clinical significanceThe combination of amikacin and Dispersin B in a degradable hydrogel could allow local treatment of complex infections without the requirement for multiple invasive procedures.
ACOUSTICAL STANDARDS NEWS
Authors: Blair-DeLeon Nancy A. , Struck Christopher J. 
Published: April 2020

American National Standards (ANSI Standards) developed by Accredited Standards Committees S1, S2, S3, S3/SC1, and S12 in the areas of acoustics, mechanical vibration and shock, bioacoustics, animal bioacoustics, and noise, respectively, are published by the Acoustical Society of America (ASA). In addition to these standards, ASA publishes a catalog of Acoustical American National Standards. To browse the latest copy of our catalog, visit our website at https://acousticalsociety.org/download-catalog/.
Comments are welcomed on all material in Acoustical Standards News.
This Acoustical Standards News section in JASA, as well as the national catalog of Acoustical Standards and other information on the Standards Program of the Acoustical Society of America, are also available via the ASA Standards home page: https://acousticalsociety.org/acoustical-society-standards/.
A Laurentian margin subduction perspective: Geodynamic constraints from phase equilibria modeling of barroisite greenstones, northern USA Appalachians
Authors: Honsberger I.W. , Laird J. , Johnson J.E. 
Published: April 2020

Abstract
Phase equilibria modeling of sodic-calcic amphibole-epidote assemblages in greenstones in the northern Appalachians, USA, is compatible with relatively shallow subduction of the early Paleozoic Laurentian margin along the Laurentia-Gondwana suture zone during closure of a portion of the Iapetus Ocean basin. Pseudosection and isopleth calculations demonstrate that peak metamorphic conditions ranged between 0.65 GPa, 480 °C and 0.85 GPa, 495 °C down-dip along the subducted Laurentian continental margin between ∼20 km and ∼30 km depth. Quantitative petrological data are explained in the context of an Early Ordovician geodynamic model involving shallow subduction of relatively young, warm, and buoyant Laurentian margin continental-oceanic lithosphere and Iapetus Ocean crust beneath a relatively warm and wet peri-Gondwanan continental arc. A relatively warm subduction zone setting may have contributed to the formation of a thin, ductile metasedimentary rock-rich channel between the down-going Laurentian slab and the overriding continental arc. This accretionary channel accommodated metamorphism and tectonization of continental margin sediments and mafic volcanic rocks (greenstones) of the Laurentian margin and provided a pathway for exhumation of serpentinite slivers and rare eclogite blocks. Restricted asthenospheric flow in the forearc mantle wedge provides one explanation for the lack of ophiolites and absence of a well-preserved ultra-high-pressure terrane in central and northern Vermont. Exhumation of the subducted portion of the Laurentian margin may have been temperature triggered due to increased asthenospheric flow following a slab tear at relatively shallow depths.
Eversion technique versus conventional endarterectomy with patch angioplasty in carotid surgery: protocol for a systematic review with meta-analyses and trial sequential analysis of randomised clinical trials
Authors: Marsman Martijn S. , Wetterslev Jorn , Vriens Patrick W.H.E. , Bleys Ronald L.A.W. , Jahrome Abdelkarime Kh. , Moll Frans L. , Keus Frederik , Koning Giel G. 
Published: April 2020

IntroductionTraditional carotid endarterectomy is considered to be the standard technique for prevention of a new stroke in patients with a symptomatic carotid stenosis. Use of patch angioplasty to restore the arterial wall after longitudinal endarterectomy is, to date, not unequivocally proven to be superior to eversion technique. A systematic review is needed for evaluation of benefits and harms of the eversion technique versus the traditional endarterectomy with patch angioplasty in patients with symptomatic carotid stenosis.Methods and outcomesThe review will be conducted according to this protocol following the recommendations of the ‘Cochrane Handbook for Systematic Reviews’ and reported according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses. Randomised clinical trials comparing eversion technique versus endarterectomy with patch angioplasty in patients with a symptomatic stenosis of the internal carotid artery will be included. Primary outcomes are all-cause mortality rate, health-related quality of life and serious adverse events. Secondary outcomes are 30-day stroke and mortality rate, symptomatic arterial restenosis or occlusion and non-serious adverse events. The databases Cochrane Central Register of Controlled Trials, PubMed/MEDLINE and EMBASE will be searched (November 2019). We will primarily base our conclusions on meta-analyses of trials with overall low-risk of bias. We will use trial sequential analysis to assist the evaluation of imprecision in Grading of Recommendations, Assessment, Development and Evaluation. However, if pooled point estimates of all trials are similar to pooled point estimates of trials with overall low risk of bias and there is lack of a statistical significant interaction between estimates from trials with overall high risk of bias and trials with overall low risk of bias we will consider the trial sequential analysis adjusted precision of the estimate achieved in all trials as the result of our meta-analyses.Ethics and disseminationThe proposed systematic review will collect and analyse data from published studies, therefore, ethical approval is not required. The results of the review will be disseminated by publication in a peer-review journal and submitted for presentation at conferences.PROSPERO registration numberCRD42019119361.
Insight in the development of the mutual trust relationship between trainers and trainees in a workplace-based postgraduate medical training programme: a focus group study among trainers and trainees of the Dutch general practice training programme
Authors: Bonnie Linda H.A. , Visser Mechteld R.M. , Kramer Anneke W.M. , van Dijk Nynke 
Published: April 2020

ObjectivesTrust plays an important role in workplace-based postgraduate medical education programmes. Trainers must trust their trainees for granting them greater independence. Trainees must trust their trainer for a safe learning environment. As trainers’ and trainees’ trust in each other plays an important role in trainee learning and development, the authors aimed to explore the development of the mutual trust relationship between trainers and trainees.SettingThis study was performed in a general practice training department in the Netherlands.ParticipantsAll trainers and trainees of the general practice training department were invited to participate. Fifteen trainers and 34 trainees, voluntarily participated in focus group discussions.Outcome measuresThe authors aimed to gain insight in the factors involved in the development of the mutual trust relationship between trainers and trainees, in order to be able to create a model for the development of a mutual trust relationship between trainers and trainees. The risk-based view of trust was adopted as leading conceptual framework.ResultsIn the first stage of trust development, trainers and trainees develop basic trust in each other. Basic trust forms the foundation of the trust relationship. In the second stage, trainers develop trust in trainees taking into account trainees’ working and learning performance, and the context in which the work is performed. Trainees trust their trainer based on the trainer’savailability and accessibility and the personal relationship between the trainee and their trainer. Trainee self-confidence modifies the development of a trust relationship.ConclusionThe development of a mutual trust relationship between trainers and trainees is a complex process that involves various stages, goals, factors and interactive aspects. As the mutual trust relationship influences the learning environment for trainees, greater emphasis on the mutual trust relationship may improve learning outcomes. Further research may explore the effect of long-term and short-term educational relationships on the trust relationship between trainers and trainees.
The EZH2–PHACTR2–AS1–Ribosome Axis induces Genomic Instability and Promotes Growth and Metastasis in Breast Cancer
Authors: Chu Wenhui , Zhang Xi , Qi Lihua , Fu Yenan , Wang Peng , Zhao Wei , Du Juan , Zhang Jing , Zhan Jun , Wang Yunling , Zhu Wei-Guo , Yu Yu , Zhang Hongquan 
Published: April 2020

Abstract

Aberrant activation of histone methyltransferase EZH2 and ribosome synthesis strongly associate with cancer development and progression. We previously found that EZH2 regulates RNA polymerase III–transcribed 5S ribosomal RNA gene transcription. However, whether EZH2 regulates ribosome synthesis is still unknown. Here, we report that EZH2 promotes ribosome synthesis by targeting and silencing a long noncoding RNA PHACTR2-AS1. PHACTR2-AS1 directly bound ribosome DNA genes and recruited histone methyltransferase SUV39H1, which in turn triggered H3K9 methylation of these genes. Depletion of PHACTR2-AS1 resulted in hyperactivation of ribosome synthesis and instability of ribosomal DNA, which promoted cancer cell proliferation and metastasis. Administration of PHACTR2-AS1-30nt-RNA, which binds to SUV39H1, effectively inhibited breast cancer growth and lung metastasis in mice. PHACTR2-AS1 was downregulated in breast cancer patients, where lower PHACTR2-AS1 expression promoted breast cancer development and correlated with poor patient outcome. Taken together, we demonstrate that PHACTR2-AS1 maintains a H3K9 methylation-marked silent state of ribosomal DNA genes, comprising a regulatory axis that controls breast cancer growth and metastasis.

Significance:
These findings reveal that EZH2 mediates ribosomal DNA stability via silencing of PHACTR2-AS1, representing a potential therapeutic target to control breast cancer growth and metastasis.
The South African genus Lepthercus Purcell, 1902 (Araneae: Mygalomorphae): phylogeny and taxonomy
Authors: RÍOS-TAMAYO DUNIESKY , LYLE ROBIN 
Published: April 2020

After more than a century, the genus Lepthercus Purcell, 1902 is revised. Lepthercus dregei Purcell, 1902 and L. rattrayi Hewitt, 1917 are redescribed; with the female of L. dregei described for the first time. Nine new species of Lepthercus are described. A phylogenetic analysis with morphological characters using implied weights and parsimony as optimality criteria, suggests the separation of the genus in two clades. The first clade is formed by L. dippenaarae sp. nov., L. engelbrechti sp. nov., L. haddadi sp. nov., L. rattrayi Hewitt, 1917 and L. sofiae sp. nov., here denominated “Group haddadi”. The species L. confusus sp. nov., Lepthercus dregei Purcell, 1902, Lepthercus filmeri sp. nov., Lepthercus kwazuluensis sp. nov., Lepthercus lawrencei sp. nov. and Lepthercus mandelai sp. nov., form the second clade, here denominated “Group dregei”. The “Group haddadi” is characterized by males with a curved metatarsus I, and a swollen tibia I. The “Group dregei” is supported by the presence of small maxillary cuspules in males. A new diagnosis is provided for Lepthercus as well as an identification key for all species of the genus. New distribution maps for the genus in the country are also presented.
Depression, trauma and mentalizing: No influence of depressive symptoms on spontaneous theory of mind in a subclinical sample
Authors: Hudson Anna R , Orlemann Corinne , Van Tricht Tine , Brass Marcel , Mueller Sven C 
Published: April 2020

Background: Depressive symptoms are associated with impaired social functioning, arguably because of reduced mentalizing abilities. However, findings in persons with depressive symptoms and/or major depressive disorder (MDD) are presently mixed, finding evidence both for and against the hypothesis of reduced mentalizing abilities. Aims: This study investigated spontaneous cognitive mentalizing in 36 age-, sex- and education-matched students with depressive symptoms and 45 comparisons with minimal depressive symptoms. Method: To assess spontaneous mentalizing, we used the implicit theory of mind (ToM) task, which looks specifically at spontaneous computation of false belief. Results: Bayesian analysis did not support the hypothesis of impaired mentalizing; in fact, it suggested that the results were 3.90 times more likely to have occurred under the null hypothesis. Results remained stable when comparing depressed and non-depressed individuals without maltreatment exposure but were inconclusive in the maltreatment-exposed groups. Conclusion: The findings suggest no effect of spontaneous mentalizing in a high-functioning depressed sample. Moreover, the findings also emphasize the need to control for childhood maltreatment experiences in future ToM and social functioning research, as these may constitute subgroups within depressed samples. Tailored therapy for maltreated depression individuals may be beneficial.
Fixation of orbicularis oculi muscle and orbital septum to pretarsal fascia and correction of blepharoptosis in construction of double eyelids
Authors: Dong Ziying , Meng Pingping , Zhang Lei , Chen Zhenyu 
Published: April 2020

Objective This study was performed to evaluate a novel method of constructing double eyelids by fixation of the orbicularis oculi muscle and orbital septum to the pretarsal fascia and correction of blepharoptosis. Methods In total, 285 patients requesting aesthetic construction of double eyelids were divided into three groups: those treated by the modified method (Group A, n = 108), those treated by traditional construction of the upper eyelid (Group B, n = 85), and those treated by the Park method (Group C, n = 92). The patients were followed up for 3 to 12 months (average, 6 months). The surgical effects and degree of satisfaction were compared among the three groups. Results In Group A, the mean operative time was 1.0 ± 0.2 hours. The degree of satisfaction with the surgical effect was significantly different between Group A (95.37%) and Group B (87.06%). However, no significant difference in satisfaction was noted between Group A (95.37%) and Group C (91.30%) or between Group B (87.06%) and Group C (91.30%). Conclusions The modified technique is simple and effective for construction of double eyelids and correction of blepharoptosis. All patients were satisfied with the surgical and aesthetic effects.