bluuebunny/crossref_metadata_2025_split huggingface dataset:
Yap Promotes Epithelial Cell Proliferation and Epithelium-derived Innate Cytokine Expression via the NF-κb Pathway in Chronic Rhinosinusitis With Nasal Polyps
Authors: Deng Huiyi , Li Meijiao , Zheng Rui. , Qiu Huijun , Yuan Tian , Wang Weihao , Chen Zhuanggui , Yang Qintai , Long Zijie , Huang Xuekun 
Published: June 2020

Abstract
Objectives: The hippo-yes-associated protein (YAP) pathway plays an important role in epithelial cell proliferation and inflammation in chronic rhinosinusitis with nasal polyps (CRSwNP). However, the underlying mechanisms remain unclear. This study intends to investigate the role of YAP and the nuclear factor kappa-B (NF-κB) signalling pathway in nasal epithelial cell proliferation and the expression of epithelium-derived cytokines in CRSwNP.Methods: The expression levels of YAP, TEAD1, Ki-67, and NF-κB in sinonasal mucosa, primary nasal epithelial cells (NPECs), and human nasal epithelial RPMI 2650 cells were detected by RT-qPCR and immunoblotting. NPECs were cultured and treated with verteporfin (VP), a selective YAP inhibitor, YAP shRNA or BAY 11-7082, a small molecule inhibitor of NF-κB. The relationship between cell proliferation and hippo pathway activity was explored using a cell counting kit-8 (CCK-8) assay, 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) labelling and colony formation assay. The cell cycle and apoptosis were examined through flow cytometry (FCM) assay. The epithelium-derived cytokines including interleukin (IL-) 33, IL-25 and thymic stromal lymphopoietin(TSLP) were detected by RT-qPCR.Results: The hippo pathway effector YAP, Ki-67, p65 NF-κB, and cyclin D1were significantly increased in CRSwNP compared with control mucosa; which was accompanied by overexpression of interleukin (IL)-33, IL-25, and thymic stromal lymphopoieth (TSLP). Pharmaceutical inhibition of YAP by VP suppressed cell proliferation of RPMI 2650 cells by blocking cell cycle progression at G0/G1 without inducing obvious cell apoptosis. Furthermore, lentiviral transfection-mediated knockdown of hippo pathway activity reduced the expression of IL-33,,IL-25, TSLP as well as p65 NF-κB in RPMI 2650 cells. Downregulation of NF-κB pathway with BAY 11-7082 in NPECs could decrease the mRNA level of TSLP, IL-33 and IL-25 accordingly.Conclusions: Inhibition of hippo pathway suppressednasal epithelial cell proliferation and declined the expression of epithelium-derived cytokines IL-33 and IL-25 and TSLP expression via the NF-κB signalling pathway in NPECs.
Mechanism of PQQ and CoQ10 overproduction in Methylobacterium as revealed by comparative genomic analysis between mutant and wild-type strains
Authors: Zhao Changle , Wan Yinping , Cao Xiaojie , Zhang Huili , Bao Xin 
Published: June 2020

Abstract
Background

The microbial synthesis of pyrroloquinoline quinone (PQQ) and Coenzyme Q10 (CoQ10) remains the most promising industrial production route. Methylobacterium has been used to generate PQQ and other value-added chemicals from cheap carbon feedstocks.However, the low PQQ and CoQ10 production capacity of the Methylobacterium strains is a major limitation The regulation mechanism for PQQ and CoQ10 biosynthesis in this strain has also not been fully elucidated.
Results

Methylobacterium sp. CLZ strain was isolated from soil contaminated with chemical wastewater, which can simultaneously produce PQQ, CoQ10, and carotenoids by using cheap methanol as carbon source. We investigated a mutant strain NI91, which increased the PQQ and CoQ10 yield by 72.44% and 59.80%, respectively. Whole-genome sequencing of NI91 and wild-type strain CLZ revealed that both contain a 5.28 Mb chromosome. The comparative genomic analysis and validation study revealed that a significant increase in biomass and PQQ production was associated with the base mutations in the methanol dehydrogenase (MDH) synthesis genes, mxaD and mxaJ. The significant increase in CoQ10 production may be associated with the base mutations in dxs gene, a key gene in the MEP/DOXP pathway.
Conclusions

A PQQ producing strain that simultaneously produces CoQ10 and carotenoids was selected and after ANI analysis, named as Methylobacterium sp. CLZ. After random mutagenesis of this strain, we obtained NI91 strain, which showed increased production of PQQ and CoQ10. Based on comparative genomic analysis of the whole genome of mutant strain NI91 and wild-type strain CLZ, a total of 270 SNPs and InDels events were detected, which provided a reference for subsequent research. The mutations in mxaD, mxaJ and dxs genes may be related to the high yield of PQQ and CoQ10. These findings will enhance our understanding of the PQQ and CoQ10 over-production mechanism in Methylobacterium sp. NI91 at the genomic level. It will also provide useful clues for strain engineering in order to improve the PQQ and CoQ10 production.
Treatment Outcome Of Malaria And Associated Factors Among Adults Admitted In Arba Minch General Hospital 2015-2018, Southern Nation Nationality And People Region, Ethiopia
Authors: Yesera Gesila Endashaw , Asfaw Hussen Mekonnen , Bekele Solomon Kassa , Dinegd Negalegn e Getahun 
Published: June 2020

Abstract
Background: Malaria is a protozoan disease transmitted by the bite of infected female Anopheles mosquitoes. Progression to severe and fatal disease is largely but not entirely confined to Plasmodium falciparum infections. Despite remarkable progress in recent years, malaria remains a leading cause of sickness and death across much of sub-Saharan Africa. Malaria disproportionately impacts the rural poor, typically people who must walk for miles to seek treatment. Methods: Institution based retrospective cross-sectional study was conducted in Arba Minch General Hospital on February 2019. Data was collected from patient record whom admitted with severe malaria in the past 4 years from Sep. 2015 – September 2018. Data was entered to Epidata 3.1. And then exported to SPSS version 25 for analysis. Bivariate and multivariate logistic regression was computed to assess statistical association between the outcome variable and independent variables using Odds Ratio; significant of statistical association was tested using 95% confidence interval (CI) and p value (<0.05). Result: Out of 422 patients with severe malaria intended to be included in the study, 387 cases were actually involved with response rate of 91.7%. The mortality rate associated with severe malaria in the year between 2015-2018 at Arba Minch General Hospital was 5.7%. Co-morbidity, impaired consciousness and acidosis were significantly associated with mortality, at significant level of P <0.05. Conclusion: co-morbidity, impaired consciousness and Acidosis were found to be poor prognostic indicators for patients with severe malaria, so priority should be given for those patients who came with such manifestations.
Impact of COVID-19 on Neurological Manifestations: An Overview of Stroke Presentation in Pandemic.
Authors: Fatima Nida , Saqqur Maher , Shauib Ashfaq 
Published: June 2020

Abstract
Introduction: Corona virus disease 2019 (COVID-19) pandemic has become a globally challenging issue after its emergence in December 2019 from Wuhan, China. Despite its common presentation as respiratory distress, patients with COVID-19 have also shown neurological manifestation especially stroke. Therefore, the authors sought to determine the etiology, underlying risk factors, and outcomes among patients with COVID-19 presenting with stroke. Methods: We conducted a systematic review of the electronic database (PubMed, Google Scholar, Scopus, Medline, EMBASE, and Cochrane library) using different MeSH terms from January 2000 to June 2020. Results: A total of 39 patients with stroke from 6 studies were included. The mean age of our included patients was 61.4±14.2 years. Majority of the patients (92.3%) with COVID-19 had ischemic stroke, 5.1% had hemorrhagic stroke, and 2.6% had cerebral venous thrombosis at the time of initial clinical presentation. Almost all of the patients presented had underlying risk factors predisposing to stroke which included, diabetes mellitus, hyperlipidemia, hypertension, and previous history of cerebrovascular disease. 51.2% of the included patients infected with COVID-19 with stroke died, while remaining patients were either discharged home or transferred to a rehabilitation unit. Conclusion: Exploring the neurological manifestation in terms of stroke among patients with COVID-19 is a step towards better understanding of the virus, preventing further spread, and treating the patients affected by this pandemic.
Detection of Leishmania infantum DNA in Pintomyia evansi and Lutzomyia longipalpis in an endemic area of Non-ulcerative cutaneous leishmaniasis from Honduras
Authors: Sosa-Ochoa Wilfredo Humberto , Amador Javier Varela , Lozano-Sardaneta Yokomi N , Segura Gabriela Rodriguez , Zúniga Concepcion , Araujo Gabriela V. , Sandoval Carmen M. , Laurenti Márcia D. , Galvis-Ovallos Fredy 
Published: June 2020

Abstract
Background The two most abundant sand flies species in Honduran Pacific coast are Lutzomyia (Lutzomyia) longipalpis and Pintomyia (Pintomyia) evansi. Both species are proved vectors of the agent of for visceral leishmaniasis in South America. Although, visceral and cutaneous non-ulcerated leishmaniasis are endemic of the Central American Pacific, being the Non-ulcerative cutaneous leishmaniasis the most frequent manifestation. In this study, we evaluate the circulation of Leishmania spp in sand flies species in the El Tigre Island, an endemic area of Visceral and Non -ulcerative cutaneous leishmaniasis in Honduras. Results Six sand flies species were identified, being Lu. longipalpis (81%) and Pi. evansi (16%) the more abundant species. Leishmania (Leishmania) infantum DNA was found in 9 of the 96 specimens analyzed, 7 of these specimens was identify as Lutzomyia (Lutzomyia) longipalpis and 2 as Pintomyia (Pifanomyia) evansi, with an infection rate of 9.4% and 2.7% respectively. Conclusion Our results present the first record of Leishmania (L.) infantum DNA in Pintomyia (Pintomyia) evansi in a Non-ulcerative cutaneous leishmaniasis endemic region from Central America. Considering the natural infection of Lu. longipalpis our results suggest that Pintomyia (Pifanomyia) evansi might be a secondary vector of Leishmania (L.) infantum and probably involved in the disease’s transmission cycle. Undoubtedly, the detection of natural infections of in this region contributes to the understanding of the L. (L.) infantum infection epidemiology in Honduras
Active Tunable THz Metamaterial Array Implemented in CMOS Technology
Authors: Liu Yongshan , Sun Tong , Wu Xiaojun , Bai Zhongyang , Sun Yun , Li Helin , Zhang Haoyi , Chen Kanglong , Ruan Cunjun , Sun Yuzu , Hu Yuanqi , Nie Tianxiao , Wen Lianggong 
Published: June 2020

Abstract
Terahertz (THz) metamaterial modulators offer multifaceted capabilities for various practical applications such as THz imaging, wireless communications, sensing, et cetera. However, compared to the modulation devices for other electromagnetic bands, the ubiquitous proliferation of THz applications is severely impeded by the tremendous lack of complementary metal-oxide-semiconductor (CMOS) compatible technology. Here we demonstrate a CMOS based actively tunable THz metamaterial array (C-ATTMA) with split ring resonators (SRRs). The THz metamaterial modulators can be externally controlled with an electrically-controlled dynamic. The C-ATTMA fabricated by the 180 nm CMOS technology featuring a resonant frequency of 0.30 THz was connected to the source and drain of a bottom metal-oxide-semiconductor field effect transistor (MOSFET) through the vias. By delicately controlling the MOSFET gate voltage, the equivalent circuit response of the C-ATTMA was actively engineered, enabling tailoring THz resonance frequencies. Under a gate voltage of 1.8 V, we successfully realized a 35 GHz modulation bandwidth with [[EQUATION]] phase modulation. The exhibited CMOS-compatible electrically-regulated THz metamaterials may provide enormous potentials for implementing THz wireless communications, information encryption, THz compressed sensing imaging, et cetera.
Compressive Test Characteristics and Constitutive Relation of Wet Polypropylene Macrofibers Shotcrete
Authors: LI Fenghui , Cheng Yunhai , Wu Fei , Su Chang , Li Gangwei 
Published: June 2020

Abstract
Shotcrete is often subject to poor ductility and cracking problems, particularly under high stresses. To address these issues, we investigated the feasibility of adding polypropylene macrofibres to shotcrete. To evaluate the supporting effect, we used dry shotcrete, wet shotcrete, and wet polypropylene macrofibre shotcrete as samples. We compared and analysed the mechanical response characteristics thereof in uniaxial compression tests by acoustic emission monitoring. The results showed that the three materials were brittle, but the ductility, residual strength, and bearing capacity of polypropylene macrofibre shotcrete were significantly enhanced. The energy absorption value of plain shotcrete was higher in the cracking stage, while that of polypropylene macrofibre shotcrete was higher in the post-peak stage, which indicated that the polypropylene macrofibre shotcrete had the characteristics of a high crack-initiation strength and toughness. Besides, the energy release from fibre shotcrete occurred after the peak stress rather than near the peak stress. The average energy absorbed by polypropylene macrofibre shotcrete was significantly higher than that in dry shotcrete and wet shotcrete, which suggested that polypropylene macrofibre shotcrete could mitigate the brittle instability of a shotcrete layer. Based on the test data, a constitutive model of damage statistics was established. The comparison between the experimental data and the fitting results could reflect the characteristics of the total stress-strain curve of such shotcrete. The results provide a basis for the optimisation of polypropylene macrofibre shotcrete layers.
Methicillin-Resistant Staphylococcus aureus Nasal Carriage among Janitors Working in Hospital and Non-Hospital Areas: A Comparative Cross-Sectional Study
Authors: Abie Seid , Tiruneh Moges , Abebe Wondwossen 
Published: June 2020

Abstract
Background: Nasal colonization of Methicillin-resistant Staphylococcus aureus (MRSA) plays a key role in the epidemiology and pathogenesis of both healthcare-associated and community-acquired MRSA infections in various populations. Screening of MRSA nasal colonization is important in the prevention and control of infection and may provide useful information to guide antimicrobial therapy. This study aimed to determine nasal carriage of MRSA, its antimicrobial susceptibility pattern, and associated factors among janitors working in hospital & non-hospital areas at the University of Gondar, Northwest Ethiopia.Methods: A comparative cross-sectional study was carried out in a total of 436 study participants (221 hospital and 215 non-hospital janitors) from January to May 2019. The study participants were sampled using a simple random sampling technique. Data on socio-demographic characteristics and associated factors were collected through face to face interviews using a structured questionnaire. Nasal swabs were collected and inoculated into Mannitol salt agar. MRSA was detected using cefoxitin (30µg) disc and an antibiotic susceptibility test was done using the disc diffusion method. Data were entered and analyzed using SPSS version 20 statistical package. P-value ≤ 0.05 was considered as statistically significant.Results: The overall prevalence of S. aureus was 101/436 (23.2%, [95% CI: 19.3-27.8], of which, 29.4% (65/221) were isolated from hospital and 16.7% (36/215) non-hospital janitors. The prevalence of MRSA was 4.8% (21/436) [95% CI: 3.0-6.9]; of these, 8.1% (18/221) of the isolates were from the hospital and 1.4% (3/215) non-hospital janitors, while MSSA in hospital & non-hospital janitors were 49 (22.2%) and 31 (14.4%), respectively. Among the MRSA isolates, 52.4% (11/21) were multi-drug resistant. Of these, 42.9% (9/18) were isolated from hospital and 66.7% (2/3) non-hospital janitors. Hence, nasal carriage of MRSA was significantly associated with hospitalization within the preceding year (AOR = 3.15, CI = 1.13-8.71).Conclusion: The present study revealed that high MSSA and MRSA were isolated from the hospital as compared to non-hospital janitors and high rates of antibiotics resistance were recorded in the hospital janitors. Consequently, hospitalizations were significantly associated with MRSA. Accordingly, regular screening of carriers in apparently healthy janitors is required for the prevention of nosocomial infections.
Circ_0004913 Sponges miR-1290 and Regulates FOXC1 to Inhibit the Proliferation of Hepatocellular Carcinoma
Authors: Yu Yabin , Han Suyang , Li Meng , Song Yan , Qi Fuzhen 
Published: June 2020

Abstract
Background Circular RNA (circRNA), a novel type of non-coding RNA, could interact with miRNA and protein molecules to regulate the occurrence and progression of hepatocellular carcinoma (HCC). However, little is known about the pathogenesis of circ_0004913 in HCC.Materials Through the GEO (Gene Expression Omnibus database) to find dysfunctional circRNAs in HCC, and circ_0004913 was selected as the research object. Quantitative reverse transcription PCR (qRT-PCR) was used to detect the expression level of circ_0067934 in HCC tissues and cells. CCK-8, Edu and flow cytometry assays were chosed to determine the malignant behavior of transfected HCC cells. Mechanistically, RNA immunoprecipitation and dual-luciferase reporter gene assay were performed to explore the relation between circ_0067934, miR-1290 and FOXC1 (Forkhead box C1) in HCC.Results The expression of circ_0004913 was down-regulated in HCC tissues and cell lines, while the overexpression of circ_0004913 attenuates the malignant behavior of HCC cells. Bioinformatics predicted that circ_0004913 interacts with miR-1290, which targeted FOXC1 mRNA. In fact, miR-1290 promoted the malignant behavior of HCC cells, while FOXC1 had the opposite effect. In addition, circ_0004913 overexpression enhanced FOXC1 expression by reducing miR-1290 expression, thereby inhibiting the proliferation of HCC cells.Conclusion Circ_0004913 / miR-1290 / FOXC1 regulatory axis could inhibit the progress of HCC. Our findings may provide potential new targets for the diagnosis and treatment of HCC.
Integrated Transcriptome and Proteome Analysis Reveals Potential Mechanisms for Differential Abdominal Fat Deposition Between Divergently Selected Chicken Lines
Authors: Wang Lijian , Leng Li , Ding Ran , Gong Pengfei , Liu Chang , Wang Ning , Li Hui , Du Zhi-Qiang , Cheng Bohan 
Published: June 2020

Abstract
Background: Genetic selection for meat production performance of broilers concomitantly causes excessive abdominal fat deposition, accompanied by several adverse effects, such as the reduction of feed conversion efficiency and reproduction performance. Our previous studies have identified important genes regulating chicken fat deposition, using the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) as an animal model. However, the molecular mechanism underlying fat deposition differences between fat and lean broilers remains largely unknown. Results: Here, we integrated the transcriptome (RNA-Seq) and quantitative proteome (isobaric tags for relative and absolute quantitation, iTRAQ) profiling analyses on abdominal fat tissues from NEAUHLF chicken lines. Differentially expressed genes (466 DEGs) and proteins (231 DEPs) were identified, and enriched in pathways related to fatty acid metabolism, fatty acid biosynthesis, glycerophospholipid metabolism, and PPAR signaling, and in pathways mainly involved in protein processing, endocytosis and lipid metabolism, respectively. Moreover, several key DEGs and DEPs involved in long-chain fatty acid uptake, in situ lipogenesis (fatty acid and cholesterol synthesis), and lipid droplets accumulation were discovered, and most of them were up-regulated in the fat line, after integrated transcriptome and proteome analysis. Conclusions: Together, our findings provided a novel insight into abdominal fat content discrepancy between the fat and lean chicken lines.
Knowledge among the rural parents about the vaccinations and vaccination coverage of children in the first year of life in Papua New Guinea – analysis of data provided by Christian Health Services
Authors: Gowin Ewelina , Kuzma Jerzy , Januszkiewicz-Lewandowska Danuta 
Published: June 2020

Abstract
Knowledge among the rural parents about the vaccinations and vaccination coverage of children in the first year of life in Papua New Guinea – analysis of data provided by Christian Health Services.BackgroundThe aim of this analysis was to establish knowledge among the rural parents about the vaccinationsvaccination coverage of children in the first year of life in Papua New Guinea (PNG).Methods56 parents including 52 mothers and 4 fathers from 2 areas Simbu Province (Kervagi district) and Morobe Province (Finschhafen district). We analysed knowledge among the rural parents about the diseases prevented by vaccinations. data on vaccination coverage from 2016 to 2018 from all Catholic health facilities. Analysed vaccinations were the pentavalent vaccine (DTaP-HiB-HepB) and measles vaccine given in the first year of life.ResultsDuring the analysed period a total of 25,502 doses of measles vaccine was given, 31,428 children were vaccinated with the pentavalent vaccine. In 2016, the coverage rate for the measles vaccine was 26.6% and 33.4% for the pentavalent vaccine. In 2017, the coverage rate for measles and pentavalent vaccines was 12.5% and 16.6%, respectively. There were big differences in immunization coverage between provinces. In 2016, greater than 80% coverage was noted in one province for measles vaccine and in four provinces for pentavalent vaccine. In 2017, in none of the provinces, the coverage rate, neither for pentavalent nor for measles vaccine, was higher than 80%. A decreasing trend in the number of administered vaccinations was observed.ConclusionThe results of this analysis demonstrate that in PNG, the majority of children are not fully immunised. There are big differences in the vaccination coverage between provinces. As protection from diseases is low, there is a very high risk of an outbreak of the vaccine-preventable disease in the community.Delivery of vaccinations in PNG encounters many barriers from access to healthcare services to natural disasters and inter-tribial conflicts.Trial registration NA
Na2co3 and Er2o3 Modified Lead Free Knn Piezoelectric Ceramic Obtained by Hot Pressing
Authors: Rutkowski Paweł , Huebner Jan , Graboś Adrian , Kata Dariusz , Sapiński Bogdan 
Published: June 2020

Abstract
Analysis of dense KNN ceramic obtained by hot pressing (HP) methodat 1100°C are presented in this paper. The synthesis of KNN-based piezoelectrics meets following challenges: low density of material, uncontrolled K/Na ratio, multiphase composition and formation of different KNN structures. Classical hot pressing approach results incontaminationby carbon originating from graphite moulds. The proposed h-BN protection layer between green sample and graphite mouldprevented carbon reaction with powder. Additionally, h-BN presence may decrease formation of oxygen vacancies which allows to maintain semiconductor features of KNN structure. Remaining issues were addressed with the addition of excess Na and Er2O3 doping. The results showed that excessNa addition allowed to compensate evaporation of sodium during the synthesis and sintering. Er2O3 was added as sintering aid to limit abnormal grain growth. The modification of amount of Na and Er2O3 addition resulted in high purity KNN samples with tetragonal structure andapparent density higher than 97%. Finally, piezoelectric features of prepared dense samples were measured and presented.