bluuebunny/crossref_metadata_2025_split huggingface dataset:
Live monitoring of ROS-induced cytosolic redox changes with roGFP2-based sensors in plants
Authors: Ugalde José Manuel , Fecker Lara , Schwarzländer Markus , Müller-Schüssele Stefanie J. , Meyer Andreas J. 
Published: December 2020

AbstractPlant cells produce reactive oxygen species (ROS) as by-products of oxygen metabolism and for signal transduction. Depending on their concentration and their site of production, ROS can cause oxidative damage within the cell, and must be effectively scavenged. Detoxification of the most stable ROS, hydrogen peroxide (H2O2), via the glutathione-ascorbate pathway may transiently alter the glutathione redox potential (EGSH). Changes inEGSHcan thus be considered as a proxy of the oxidative load in the cell. Genetically encoded probes based on roGFP2 enable extended opportunities forin vivomonitoring of H2O2andEGSHdynamics. Here, we report detailed protocols for live monitoring of both parameters in the cytosol with the probes Grx1-roGFP2 forEGSHand roGFP2-Orp1 for H2O2, respectively. The protocols have been adapted for live cell imaging with high lateral resolution on a confocal microscope and for multiparallel measurements in whole organs or intact seedlings in a fluorescence microplate reader. Elicitor-induced ROS generation is used as an example for illustration of the opportunities for dynamic ROS measurements that can easily be transferred to other scientific questions and model systems.
Impact of mHealth interventions on antenatal and postnatal care utilization in low and middle-income countries: A Systematic Review and Meta-Analysis
Authors: Kant Ravi , Yadav Poonam , Kishore Surekha , Barnwal Shruti , Kumar Rajesh , Khapre Meenakshi 
Published: December 2020

ABSTRACTObjectiveTo evaluate the effectiveness of mHealth interventions on antenatal and postnatal care utilization in low and middle-income countries.DesignSystematic review and meta-analysisSettingStudies from low and middle-income countries were included for analysis.ParticipantsWe searched the literature through major electronic databases such as PubMed, MEDLINE, Embase, Cochrane, Scopus, CINAHL, Clinical key, Google Scholar, Ovid databases with selected keywords, and explored the reference list of articles. Meta-analysis was performed in RevMan 5.4 software; p-value < 0.05 was considered statistically significant. The effect of variables was measured in the Odds ratio with a fixed-effect model. Six published interventional studies were selected as per the eligibility and PICO framed for the systematic review and meta-analysis. Search restricted to articles in the English language, online published, and preprint articles till September 2020.Primary and secondary outcome measuresWe evaluated the effectiveness of mHealth intervention on antenatal care utilization, including four antenatal check-ups, iron-folic acid supplementation, two tetanus toxoid immunizations, and postnatal care utilization, which includes postnatal check-ups of delivered mothers.ResultsResults have been presented in the form of a forest plot. Findings of this meta-analysis depicted the significant increase in four or more antenatal care attendance (OR=1.89, 95% CI-1.49-2.19), TT immunization (OR=1.63 (95% CI-1.17-2.27), compliance to iron supplementation (OR=1.88, 95% CI-1.18-3.00) and postnatal care attendance (OR=2.54 (95% CI-2.15-2.99) among those pregnant mothers who received mHealth intervention compared to control group.ConclusionThis meta-analysis concluded that m-health has the potential to increase the utilization of full antenatal care and postnatal care compared to standard care, although the level of evidence is moderate.Trial registrationCRD42020204618, PROSPERO, International prospective register of systematic reviewsArticle SummaryStrengths and limitations of this studyThis meta-analysis creates an evidence for the effectiveness of mHealth with pooled data of interventional studies with limited sample sizes.Technology is changing, but even with limited support like SMS, there was an improvement in antenatal and postnatal service utilization.Sensitivity analysis identified possible reasons for heterogeneity among studies.Studies included from LMICs so results can be generalized for the respective population.mHealth as an intervention is a broad term that created heterogeneity also.
SPaRTAN, a computational framework for linking cell-surface receptors to transcriptional regulators
Authors: Ma Xiaojun , Somasundaram Ashwin , Qi Zengbiao , Singh Harinder , Osmanbeyoglu Hatice Ulku 
Published: December 2020

AbstractThe identity and functions of specialized cell types are dependent on the complex interplay between signaling and transcriptional networks. We present SPaRTAN (Single-cell Proteomic and RNA based Transcription factor Activity Network), a computational method to link cell-surface receptors to transcription factors (TFs) by exploiting cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) datasets with cis-regulatory information. SPaRTAN is applied to immune cell types in the blood to predict the coupling of signaling receptors with cell context-specific TFs. The predictions are validated by prior knowledge and flow cytometry analyses. SPaRTAN is then used to predict the signaling coupled TF states of tumor infiltrating CD8+T cells in malignant peritoneal and pleural mesotheliomas. SPaRTAN greatly enhances the utility of CITE-seq datasets to uncover TF and cell-surface receptor relationships in diverse cellular states.SignificanceRecently single-cell technologies such as CITE-seq have been developed that enable simultaneous quantitative analysis of cell-surface receptor expression with transcriptional states. To date, these datasets have not been used to systematically develop cell-context-specific maps of the interface between signaling and transcriptional regulators orchestrating cellular identity and function. We developed a computational framework, SPaRTAN (Single-cell Proteomic and RNA based Transcription factor Activity Network) that integrates single-cell proteomic and transcriptomic data based on CITE-seq with cis-regulatory information. We applied our method to publicly available (PBMCs) and new CITE-seq datasets (mesothelioma). Our predicted TF activity and cell-surface receptor relationships are validated by prior knowledge as well as experimental testing. SPaRTAN reveals many previously unidentified surface-receptor associated TFs.
Integrated care effectiveness for adults with co-occurring disorders
Authors: Mooss Angela , Myatt Joyce , Goldman Jennifer , Alexander Joey-Ann 
Published: December 2020

PurposeThis study examined effectiveness of an integrated care program on emergency department visits within a longitudinal sample of patients with both primary care and behavioral health diagnoses.Design/methodology/approachPatients with co-occurring disorders enrolled in an integrated care clinic and were followed over time to determine whether participation in the clinic, including engagement in wellness/peer services, predicted decreases in Emergency Department (ED) use. Associations between socio-demographic characteristics of patients and ED use were also analyzed.FindingsAfter 6 months, clinic patients had decreases in ED use that continued for twelve months, albeit to a lesser degree. Demographics and program services were not related to ED use; however, multiple associations existed between high ED utilizers, severe mental illness (SMI), substance use disorders (SUD) and non-retention in services.Research limitations/implicationsThe study lacked a comparison group and there was no distinction between avoidable and unavoidable ED visits. A small sample size across time points led to inconclusive post hoc findings.Originality/valueThis study explored effectiveness of primary care integration into a behavioral health clinic for persons with multiple morbidities. Although initial decreases in ED visits were present, results indicate that these models may not be effective for persons with SMI or SMI/SUD who are already high ED users. This study provides support for integrated care in reducing ED use among persons with multiple morbidities and calls for further research on designing effective integrated models for persons with SMI and SUD.
Exosomal miR‐3180‐3p inhibits proliferation and metastasis of non‐small cell lung cancer by downregulating FOXP4
Authors: Chen Tengfei , Liu Yali , Chen Jun , Zheng Huifei , Chen Qiuyun , Zhao Jun 
Published: December 2020

BackgroundNon‐small cell lung cancer (NSCLC) is one of the most malignant cancers worldwide and its pathogenesis is not completely clear. In this study, we explored the functions and mechanisms of exosomes transferring miR‐3180‐3p in NSCLC progression.MethodsThe expression levels of miR‐3180‐3p in NSCLC tissues and paracarcinoma tissues was obtained from the GEO database (GEO: GSE53882). Exosomes derived from A549 cells were identified. Proliferation, migration and invasion were measured after treatment with exosomal miR‐3180‐3p or transfection using miR‐3180‐3p mimics. The relationship between miR‐3180‐3p and forkhead box P4 (FOXP4) was predicted using a bioinformatic tool and measured using a dual‐luciferase reporter gene assay and western blotting. Finally, a mouse xenograft model of NSCLC cells was established to verify the function of exosomal miR‐3180‐3p in vivo.ResultsWe found that miR‐3180‐3p decreased in both NSCLC cell lines and patient tissues. Overexpression of miR‐3180‐3p or treatment with exosomal miR‐3180‐3p significantly suppressed cell proliferation and metastasis in NSCLC cell lines. Subsequently, we found miR‐3180‐3p downregulated FOXP4 protein expression levels. Furthermore, the volumes and weights of nude mouse tumors expressing exosomal miR‐3180‐3p were significantly reduced.ConclusionsExosomal miR‐3180‐3p suppresses NSCLC progression by downregulating FOXP4 expression.Key pointsSignificant findings of the studyWe found that exosomal miR‐3180‐3p suppressed NSCLC progression and also identified a miR‐3180‐3p target gene. These findings provide a foundation to determine innovative therapeutic strategies.What this study addsThis study contributes to research investigating exosomal containing miRNAs.
Magnocellular and Parvocellular Mediated Luminance Contrast Discrimination in Severe Alcohol Use Disorder
Authors: Creupelandt Coralie , Maurage Pierre , Lenoble Quentin , Lambot Carine , Geus Christophe , D’Hondt Fabien 
Published: December 2020

BackgroundSevere alcohol use disorder (SAUD) is associated with widespread cognitive impairments, including low‐level visual processing deficits that persist after prolonged abstinence. However, the extent and characteristics of these visual deficits remain largely undetermined, impeding the identification of their underlying mechanisms and influence on higher‐order processing. In particular, little work has been conducted to assess the integrity of the magnocellular (MC) and parvocellular (PC) visual pathways, namely the 2 main visual streams that convey information from the retina up to striate, extrastriate, and dorsal/ventral cerebral regions.MethodsWe investigated achromatic luminance contrast processing mediated by inferred MC and PC pathways in 33 patients with SAUD and 32 matched healthy controls using 2 psychophysical pedestal contrast discrimination tasks that promote responses of inferred MC or PC pathways. We relied on a staircase procedure to assess participants’ ability to detect small changes in luminance within an array of 4 gray squares that were either continuously presented (steady pedestal, MC‐biased) or briefly flashed (pulsed pedestal, PC‐biased).ResultsWe replicated the expected pattern of MC and PC contrast responses in healthy controls. We found preserved dissociation of MC and PC contrast signatures in SAUD but higher MC‐mediated mean contrast discrimination thresholds combined with a steeper PC‐mediated contrast discrimination slope compared with healthy controls.ConclusionThese findings indicate altered MC‐mediated contrast sensitivity and PC‐mediated contrast gain, confirming the presence of early sensory disturbances in individuals with SAUD. Such low‐level deficits, while usually overlooked, might influence higher‐order abilities (e.g., memory, executive functions) in SAUD by disturbing the “coarse‐to‐fine” tuning of the visual system, which relies on the distinct functional properties of MC and PC pathways and ensures proper and efficient monitoring of the environment.
Plastic ingestion is an underestimated cause of death for southern hemisphere albatrosses
Authors: Roman Lauren , Butcher Richelle Grace , Stewart David , Hunter Stuart , Jolly Megan , Kowalski Phil , Hardesty Britta Denise , Lenting Baukje 
Published: December 2020

AbstractAlbatrosses are among the world's most imperiled vertebrates, with 73% of species threatened with extinction. Ingestion of plastic is a well‐recognized threat among three North Pacific species, but lesser known in the southern hemisphere, where it is considered a minor threat. As plastic entering the ocean is increasing while albatross populations decline, the threat of ocean plastic to albatross populations may be underestimated. We present case studies of 107 beach‐cast albatrosses of twelve species, received by wildlife hospitals in Australia and New Zealand, and estimate plastic ingestion and mortality rates for albatrosses in the southern hemisphere. Ingested plastic was present in 5.6% of individuals, and the cause of death in half of these cases. We estimate ingestion of plastic may cause 3.4–17.5% of nearshore mortalities and is worth consideration as a substantial threat to albatross populations. We provide clinical findings and “checklist” methodologies for identifying potential cases of foreign‐body gastrointestinal obstruction. We suggest practical policy responses, empowering decision makers to reduce albatross mortality from anthropogenic sources.
Ski promotes proliferation and inhibits apoptosis in fibroblasts under high‐glucose conditions via the FoxO1 pathway
Authors: Peng Yan , Xiong Ren‐Ping , Zhang Zhuo‐Hang , Ning Ya‐Lei , Zhao Yan , Tan Si‐Wei , Zhou Yuan‐Guo , Li Ping 
Published: December 2020

AbstractObjectivesThe present study clarified the role and signalling pathway of Ski in regulating proliferation and apoptosis in fibroblasts under high‐glucose (HG) conditions.Materials and MethodsThe proliferation and apoptosis of rat primary fibroblasts were assessed using EdU incorporation and TUNEL assays. The protein and phosphorylation levels of the corresponding factors were measured using immunofluorescence staining and Western blotting. Immunoprecipitation was used to determine the interactions between Ski and FoxO1 or Ski and HDAC1. The Ski protein was overexpressed via recombinant adenovirus transfection, and FoxO1 and HDAC1 were knocked down using targeted small‐interfering RNA.ResultsThe present study found that HG inhibited fibroblast proliferation, increased apoptosis and reduced Ski levels in rat primary fibroblasts. Conversely, increasing Ski protein levels alleviated HG‐induced proliferation inhibition and apoptosis promotion. Increasing Ski protein levels also increased Ski binding to FoxO1 to decrease FoxO1 acetylation, and interfering with FoxO1 caused loss of the regulatory effect of Ski in fibroblasts under HG. Increasing Ski protein levels decreased FoxO1 acetylation via HDAC1‐mediated deacetylation.ConclusionsTherefore, these findings confirmed for the first time that Ski regulated fibroblast proliferation and apoptosis under HG conditions via the FoxO1 pathway.
Cross‐national comparison of social isolation and mortality among older adults: A 10‐year follow‐up study in Japan and England
Authors: Saito Masashige , Aida Jun , Cable Noriko , Zaninotto Paola , Ikeda Takaaki , Tsuji Taishi , Koyama Shihoko , Noguchi Taiji , Osaka Ken , Kondo Katsunori 
Published: December 2020

AimExisting evidence links social isolation with poor health. To examine differences in the mortality risk by social isolation, and in socio‐economic correlates of social isolation, we analyzed large‐scale cohort studies in Japan and England.MethodsParticipants were drawn from the Japan Gerontological Evaluation Study (JAGES) and the English Longitudinal Study of Ageing (ELSA). We analyzed the 10‐year mortality among 15 313 JAGES participants and 5124 ELSA respondents. Social isolation was measured by two scales, i.e., scoring the frequency of contact with close ties, and a composite measurement of social isolation risk. We calculated the population attributable fraction, and Cox regression models with multiple imputations were used to estimate hazard ratios (HRs) for mortality due to social isolation.ResultsThe proportion of those with contact frequency of less than once a month was 8.5% in JAGES and 1.3% in ELSA. Males, older people, those with poor self‐rated health, and unmarried people were significantly associated with social isolation in both countries. Both scales showed that social isolation among older adults had a remarkably higher risk for premature death (less frequent contact with others in JAGES: hazard ratio [HR] = 1.18, 95% confidence interval [CI]: 1.05–1.33, in ELSA: HR = 1.27, 95% CI: 0.85–1.89; and high isolation risk score in JAGES: HR = 1.30, 95% CI: 1.12–1.50, in ELSA: HR = 2.05, 95% CI: 1.52–2.73). The population attributable fraction showed less frequent contact with close ties was attributed to about 18 000 premature deaths annually in Japan, in contrast with about 1800 in England.ConclusionsNegative health impacts of social isolation were higher among older Japanese compared with those in England. Geriatr Gerontol Int 2021; 21: 209–214.
How politicians see their relationship with top bureaucrats: Revisiting classical images
Authors: Baekgaard Martin , Blom‐Hansen Jens , Serritzlew Søren 
Published: December 2020

AbstractThe relationship between politicians and bureaucrats is an enduring concern in political science. Central to this debate, Aberbach, Putnam, and Rockman (APR) in 1981 developed four images to characterize political‐bureaucratic relations. We argue that the one‐dimensional focus on roles in their images comes with important limitations. To deal with these limitations, we collect survey responses from 3,384 local politicians from four countries on seven dimensions of the political‐bureaucratic relationship. We then use cluster analysis to develop six images bottom‐up. Five of our images are largely consistent with APR's image II and III. Yet, they differ in the extent to which politicians trust the bureaucracy, consider them loyal, and see them as an important source of information. A sixth image is not consistent with any of APR's images. We find that both systemic (country, municipality size) and individual factors (ideology, position, seniority) contribute to differences in images. Overall, our images suggest that political‐bureaucratic relations vary more between and within political systems than suggested by APR's images.
Septic arthritis due to Neisseria gonorrhoea in Western Australia
Authors: Nossent Johannes , Raymond Warren , Keen Helen , Preen David B. , Inderjeeth Charles A. 
Published: December 2020

AbstractBackgroundA high prevalence of gonococcal infections has been reported from remote parts of Western Australia, but the occurrence of disseminated infection leading to arthritis has not been studied.AimsTo investigate the frequency, risk factors and long‐term outcome of gonococcal arthritis (GA) in Western Australia (WA).MethodsA population‐based data linkage study of patients with a hospital‐based diagnosis of GA in WA between 1990 and 2014. Demographics, standardised incidence rates per million and comorbidity accrued before (lookback 186 months, interquartile range (IQR) 86–267) and after the index hospital contact for GA (follow up 100 months, IQR 60–209) are presented as frequency (%), median (IQR) or rates /1000 months.ResultsIn total, 98 patients were diagnosed with GA. The annual incidence of GA increased from 1.35 to 2.10 per million between 1990 and 2014, but the rate of GA complicating all gonococcal infections was stable around 0.25%. Female patients with GA (54%; n = 53/98) were younger (24 vs 38 years) and more frequently identified as indigenous (88% vs 49%) than male patients (46%; n = 45/98; P = 0.002). Female patients had higher rates of prior infections (15.5 vs 8.1 per 1000 months; P = 0.002) and diabetes mellitus (15.9% vs 2.5%; P = 0.03) and a longer hospital stay (10 vs 8 days; P = 0.02). GA recurrence rate during follow up was low (2%), but a broad range of comorbidities developed contributing to a 14% crude death rate.ConclusionsGA stably complicates 0.25% of gonococcal infections in WA with young indigenous females and middle‐aged non‐indigenous males most affected. Prior infectious disease and diabetes mellitus are potential risk factors for GA in females. GA recurs rarely, but its development reflects a high risk of morbidity and mortality over the following 10 years.
Circular RNA in cardiovascular disease: Expression, mechanisms and clinical prospects
Authors: Tang Ying , Bao Jinghui , Hu Jiahui , Liu Leiling , Xu Dan‐Yan 
Published: December 2020

AbstractCircular RNAs (circRNAs) are a group of covalently closed, endogenous, non‐coding RNAs, which exist widely in human tissues including the heart. Increasing evidence has shown that cardiac circRNAs play crucial regulatory roles in cardiovascular diseases (CVDs). In this review, we aimed to provide a systemic understanding of circRNAs with a special emphasis on the cardiovascular system. We have summarized the current research on the classification, biogenesis and properties of circRNAs as well as their participation in the pathogenesis of CVDs. CircRNAs are conserved, stable and have specific spatiotemporal expression; thus, they have been accepted as a potential diagnostic marker or an incremental prognostic biomarker for CVDs.
Arabidopsis U‐box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor‐like protein kinases LRR1 and KIN7
Authors: Chen Xuexue , Wang Tingting , Rehman Amin Ur , Wang Yu , Qi Junsheng , Li Zhen , Song Chunpeng , Wang Baoshan , Yang Shuhua , Gong Zhizhong 
Published: December 2020

AbstractBoth plant receptor‐like protein kinases (RLKs) and ubiquitin‐mediated proteolysis play crucial roles in plant responses to drought stress. However, the mechanism by which E3 ubiquitin ligases modulate RLKs is poorly understood. In this study, we showed that Arabidopsis PLANT U‐BOX PROTEIN 11 (PUB11), an E3 ubiquitin ligase, negatively regulates abscisic acid (ABA)‐mediated drought responses. PUB11 interacts with and ubiquitinates two receptor‐like protein kinases, LEUCINE RICH REPEAT PROTEIN 1 (LRR1) and KINASE 7 (KIN7), and mediates their degradation during plant responses to drought stress in vitro and in vivo. pub11 mutants were more tolerant, whereas lrr1 and kin7 mutants were more sensitive, to drought stress than the wild type. Genetic analyses show that the pub11 lrr1 kin7 triple mutant exhibited similar drought sensitivity as the lrr1 kin7 double mutant, placing PUB11 upstream of the two RLKs. Abscisic acid and drought treatment promoted the accumulation of PUB11, which likely accelerates LRR1 and KIN7 degradation. Together, our results reveal that PUB11 negatively regulates plant responses to drought stress by destabilizing the LRR1 and KIN7 RLKs.