bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Quality of Life Before and After Septoplasty With Short Form-36
Authors: Erdivanli Ozlem Celebi , Coskun Zerrin Ozergin , Ozgur Abdulkadir , Ogurlu Mahmut , Demirci Munir , Dursun Engin 
Published: February 2020

Abstract
The authors aimed to evaluate quality of life after septal surgery with Short Form-36 survey, and the effectiveness of the survey. Nasal symptoms (nasal obstruction, facial pain, catarrh), and general quality of life (using the Turkish version of the Short Form-36 questionnaire) were assessed preoperatively and at 1st and 6th postoperative months. Acoustic rhinometry and rhinomanometry were assessed preoperatively and at 6th postoperative month. Data from 78 patients were analyzed. This prospective clinical study was conducted on patients complaining of nasal obstruction with nasal septal deviation. Seventy-eight patients were included in the study. Fourty-two patients (53.8%) were male and 36 patients (46.2%) were female. At 1st postoperative month, nasal obstruction, facial pain and catarrh scores significantly improved in all, 46, 18 patients, respectively (P < 0.0001). At 6th postoperative month, scores nonsignificantly worsened by 1 point in 12, 6, 12 patients, respectively. Nasal volume significantly increased and total resistance significantly decreased at 6th month (P < 0.0001). Compared to preoperative values, all items except social function and bodily pain significantly increased at 1st postoperative month. Compared to values at 1st postoperative month, only bodily pain score decreased at 6th postoperative month. One patient presented with septal perforation. Septoplasty is a well-established technique to reduce nasal obstruction and improve quality of life. SF-36 may be used as a reliable measure of changes in quality of life after septal surgery.
Estimating the serial interval of the novel coronavirus disease (COVID-19): A statistical analysis using the public data in Hong Kong from January 16 to February 15, 2020
Authors: Zhao Shi , Gao Daozhou , Zhuang Zian , Chong Marc KC , Cai Yongli , Ran Jinjun , Cao Peihua , Wang Kai , Lou Yijun , Wang Weiming , Yang Lin , He Daihai , Wang Maggie H 
Published: February 2020

AbstractBackgroundsThe emerging virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused a large outbreak of novel coronavirus disease (COVID-19) in Wuhan, China since December 2019. Based on the publicly available surveillance data, we identified 21 transmission chains in Hong Kong and estimated the serial interval (SI) of COVID-19.MethodsIndex cases were identified and reported after symptoms onset, and contact tracing was conducted to collect the data of the associated secondary cases. An interval censored likelihood framework is adopted to fit a Gamma distribution function to govern the SI of COVID-19.FindingsAssuming a Gamma distributed model, we estimated the mean of SI at 4.4 days (95%CI: 2.9−6.7) and SD of SI at 3.0 days (95%CI: 1.8−5.8) by using the information of all 21 transmission chains in Hong Kong.ConclusionThe SI of COVID-19 may be shorter than the preliminary estimates in previous works. Given the likelihood that SI could be shorter than the incubation period, pre-symptomatic transmission may occur, and extra efforts on timely contact tracing and quarantine are recommended in combating the COVID-19 outbreak.
Latent Plasticity of Effector-like Exhausted CD8 T cells contributes to memory responses
Authors: Raju Saravanan , Xia Yu , Daniel Bence , Yost Kathryn E. , Bradshaw Elliot , Tonc Elena , Verbaro Daniel J. , Satpathy Ansuman T. , Egawa Takeshi 
Published: February 2020

AbstractPersistent antigen induces a dysfunctional CD8 T cell state known as T cell “exhaustion” characterized by expression of PD-1 and decreased effector functions. Nevertheless, dysfunctional CD8 T cells can mediate control of antigen burden which is long-lasting. While heterogeneity of exhausted CD8 T cells has been described, the cells which actively proliferate and exert viral control have remained elusive. Here, we define subsets of PD-1+CD8 T cells during chronic infection marked by expression of CX3CR1 with substantialin situproliferation and high expression of granzyme B. Moreover, these cells maintain the effector pool through self-renewal independently of previously defined stem-like cells. Unexpectedly, CX3CR1+CD8 T cells retain plasticity to be reprogrammed to memory cells through expression of TCF-1 and re-gain polyfunctionality. Thus, we define a subset of effector-like exhausted CD8 T cells with capacity to contribute to the memory pool, offering a prime target for novel immunotherapies.
Gender differences in patients with COVID-19: Focus on severity and mortality
Authors: Jin Jian-Min , Bai Peng , He Wei , Wu Fei , Liu Xiao-Fang , Han De-Min , Liu Shi , Yang Jin-Kui 
Published: February 2020

AbstractImportanceThe recent outbreak of Novel Coronavirus (SARS-CoV-2) Disease (COVID-19) has put the world on alert, that is reminiscent of the SARS outbreak seventeen years ago.ObjectiveWe aim to compare the severity and mortality between male and female patients with both COVID-19 and SARS, to explore the most useful prognostic factors for individualized assessment.Design, Setting, and ParticipantsWe extracted the data from a case series of 43 hospitalized patients we treated, a public data set of the first 37 cases died of COVID-19 in Wuhan city and 1019 survived patients from six cities in China. We also analyzed the data of 524 patients with SARS, including 139 deaths, from Beijing city in early 2003.Main Outcomes and MeasuresSeverity and mortality.ResultsOlder age and high number of comorbidities were associated with higher severity and mortality in patients with both COVID-19 and SARS. The percentages of older age (≥65 years) were much higher in the deceased group than in the survived group in patients with both COVID-19 (83.8 vs. 13.2, P<0.001) and SARS (37.4 vs. 4.9, P<0.001). In the case series, men tend to be more serious than women (P=0.035), although age was comparable between men and women. In the public data set, age was also comparable between men and women in the deceased group or the survived group in patients with COVID-19. Meanwhile, gender distribution was exactly symmetrical in the 1019 survivors of COVID-19. However, the percentage of male were higher in the deceased group than in the survived group (70.3 vs. 50.0, P=0.015). The gender role in mortality was also observed in SARS patients. Survival analysis showed that men (hazard ratio [95% CI] 1.47 [1.05-2.06, P= 0.025) had a significantly higher mortality rate than women in patients with SARS.Conclusions and RelevanceOlder age and male gender are risk factors for worse outcome in patients with COVID. While men and women have the same susceptibility to both SARS-CoV-2 and SARS-CoV, men may be more prone to have higher severity and mortality independent of age and susceptibility.Key PointsQuestionAre men more susceptible to getting and dying from COVID-19?FindingsIn the case series, men tend to be more serious than women. In the public data set, the percentage of men were higher in the deceased group than in the survived group, although age was comparable between men and women.MeaningMale gender is a risk factor for worse outcome in patients with COVID independent of age and susceptibility.
Effect of exercise and dietary intervention on serum metabolomics in men with insomnia symptoms: a 6-month randomized-controlled trial
Authors: Zhang Xiaobo , Wang Xiuqiang , Le Shenglong , Ojanen Xiaowei , Tan Xiao , Wiklund Petri , Cheng Sulin 
Published: February 2020

AbstractBackgroundAccumulating evidences have shown that lifestyle interventions such as exercise and diet are associated with improved sleep quality. However, the underlying molecular mechanisms remain unclear. Assessing exercise and diet intervention associated changes in circulating metabolomics profile in people with insomnia symptoms may help to identify molecular biomarkers that may link lifestyle changes to improved sleep outcomes.MethodsThe present study is a part of a 6-month randomized lifestyle intervention on sleep disorder subjects. Seventy-two Finnish men (aged: 51.6 ± 10.1 years; body mass index, BMI: 29.3 ± 3.9 kg/m2) with chronic insomnia symptoms who were assigned into different intervention groups completed this study (exercise n = 24, diet n = 27 and control n = 21). The exercise group was assigned to a progressive aerobic exercise training with intensity of 60 – 75% of estimated maximum heart rate, 3 – 5 times a week. The diet group aimed to reduce their total energy intakes by 300 to 500 kcal per day for the first three months. The control group were advised to maintain their current lifestyle. Sleep was assessed by using a non-contact sleep monitoring devise (Beddit sleep tracker). Blood samples were collected in the morning between 7:00 and 9:00 a.m. after overnight fasting. Gas Chromatography Time-Of-Flight Mass Spectrometry (GC-TOF-MS) method was used to determine the serum metabolites.ResultsTwenty-one metabolites were significantly changed in the exercise group, thirty-three metabolites in the diet group and five metabolites in the control group after intervention, respectively. The differential metabolites after exercise intervention were mainly related to glycerolipids and carbohydrates metabolism, while dietary intervention altered mainly amino acids metabolism and fatty acids metabolism related metabolites. We subsequently assessed the change of those metabolites with the change of sleep parameters and found that decreased alpha-ketoisocaproic acid (r = -0.52, p = 0.026) was correlated with improved sleep efficiency (SE) in the exercise group. Change of 3-hydroxybutric acid (r = -0.47, p = 0.025) and D-glucopyranose (r = -0.54, p = 0.006) correlated negatively with SE in the diet group. On the other hand, oxalic acid (r = 0.49, p = 0.021), D-glucopyranose (r = 0.43, p = 0.048), 4-deoxyerythronic acid (r = 0.60, p = 0.004) and tagatose (r = 0.51, p = 0.016) correlated positively with change of SOL, and 2-keto-isovaleric acid (r = 0.45, p = 0.029) correlated with TST in the diet group.ConclusionIn conclusion, this study identified circulating metabolites that may represent a part of a biological mechanism through which lifestyle interventions are associated with improved sleep quality in people with insomnia.
The anticipation and perception of affective touch in women with and recovered from Anorexia Nervosa
Authors: Crucianelli Laura , Demartini Benedetta , Goeta Diana , Nisticò Veronica , Saramandi Alkistis , Bertelli Sara , Todisco Patrizia , Gambini Orsola , Fotopoulou Aikaterini 
Published: February 2020

AbstractDisruptions in reward processing and anhedonia have long being considered as possible contributors to the aetiology and maintenance of Anorexia nervosa (AN). Recently, interoceptive deficits have also been observed in AN, including reduced tactile pleasure. However, the extent to which this tactile anhedonia is specifically liked to an impairment in a specialized, interoceptive C tactile system originating at the periphery, or a more top-down mechanism in the processing of pleasant tactile stimuli remains debated. Here, we investigated two related hypotheses. First, we examined whether the differences, between patients with AN and healthy controls in the perception of pleasantness of touch stimuli delivered in a CT-optimal manner versus a CT non-optimal manner would also be observed in patients recovered from AN. This is important as tactile anhedonia in acute patients may be the secondary result of prolonged malnutrition, rather than a deficit that contributed to the development of the disorder. Second, we examined whether these three groups would also differ in their top-down, anticipatory beliefs about the perceived pleasantness of different materials touching the skin, and to what degree such top-down beliefs and related impairments in alexithymia and interoceptive sensibility would explain any differences in perceived tactile plesantness. To this end, we measured the anticipated pleasantness of various materials touching the skin and the perceived pleasantness of light, dynamic stroking touches applied to the forearm of 27 women with AN, 24 women who have recovered and 30 healthy controls using C Tactile (CT) afferents-optimal (slow) and non-optimal (fast) velocities. Our results showed that both clinical groups anticipated tactile experiences and rated delivered tactile stimuli as less pleasant than healthy controls, but the latter difference was not related to the CT optimality of the stimulation. Instead, differences in how CT optimal touch were perceived were predicted by differences in top-down beliefs, alexithymia and interoceptive sensibility. Thus, this study concludes that tactile anhedonia in AN is not the secondary result of malnutrition but persists as a trait even after otherwise successful recovery of AN and also it not linked to a bottom-up interoceptive deficit in the CT system, but rather to a learned, defective top-down anticipation of pleasant tactile experiences.
Does Vergence Affect Perceived Size?
Authors: Linton Paul 
Published: February 2020

AbstractSince Kepler (1604) and Descartes (1637), it’s been suggested that ‘vergence’ (the angular rotation of the eyes) plays a key role in size constancy. However, this has never been tested divorced from confounding cues such as changes in the retinal image. In our experiment participants viewed a target which grew or shrank over 5 seconds. At the same time the fixation distance specified by vergence was reduced from 50cm to 25cm. The question was whether the reduction in the viewing distance specified by vergence biased the participants’ judgements of whether the target grew or shrank? We found no evidence of any bias, and therefore no evidence that eye movements affect perceived size. If this is correct, then this finding has three implications: First, perceived size is much more reliant on cognitive influences than previously thought. This is consistent with the argument that visual scale is purely cognitive in nature (Linton, 2017; 2018). Second, it leads us to question whether the vergence modulation of V1 contributes to size constancy. Third, given the interaction between vergence, proprioception, and the retinal image in the Taylor illusion, it leads us to ask whether this cognitive approach could also be applied to multisensory integration.
Replicated chromatin curtails 53BP1 recruitment in BRCA1-proficient and -deficient cells
Authors: Michelena Jone , Pellegrino Stefania , Spegg Vincent , Altmeyer Matthias 
Published: February 2020

AbstractDNA double-strand breaks can be repaired by two competing mechanisms, non-homologous end-joining (NHEJ) and homologous recombination (HR). Whether one or the other repair pathway is favored depends on the availability of an undamaged template DNA that allows for homology-directed repair. The tumor suppressor proteins 53BP1 and BRCA1 are considered antagonistic players in this repair pathway choice, as 53BP1 restrains DNA end resection, whereas BRCA1, together with its partner protein BARD1, displaces 53BP1 from damaged replicated chromatin and promotes HR. How cells switch from a 53BP1-dominated to a BRCA1-dominated response as they progress through the cell cycle is incompletely understood. Here we reveal, using high-throughput microscopy and applying single cell normalization to control for increased genome size as cells replicate their DNA, that 53BP1 recruitment to damaged replicated chromatin is inefficient in both BRCA1-proficient and BRCA1-deficient cells, in comparison to 53BP1 accumulation at damaged unreplicated chromatin. These findings substantiate a dual switch model from a 53BP1-dominated response in unreplicated chromatin to a BRCA1-BARD1-dominated response in replicated chromatin, in which replication-coupled dilution of 53BP1’s binding mark H4K20me2 functionally cooperates with BRCA1-BARD1-mediated suppression of 53BP1 binding. More generally, we suggest that appropriate normalization of single cell data, e.g. to DNA content, provides additional layers of information, which can be critical for quantifying and interpreting cellular phenotypes.
IFN-λ4 is associated with increased risk and earlier occurrence of gastrointestinal, respiratory and malarial infections in Malian children
Authors: Prokunina-Olsson Ludmila , Morrison Robert D. , Obajemu Adeola , Mahamar Almahamoudou , Kim Sungduk , Attaher Oumar , Florez-Vargas Oscar , Sidibe Youssoufa , Onabajo Olusegun O. , Hutchinson Amy A. , Manning Michelle , Kwan Jennifer , Brand Nathan , Dicko Alassane , Fried Michal , Albert Paul S. , Mbulaiteye Sam M. , Duffy Patrick E. 
Published: February 2020

ABSTRACTGenetic polymorphisms within theIFNL3/IFNL4genomic region, which encodes type III interferons, have been strongly associated with impaired clearance of hepatitis C virus (HCV) infection. We hypothesized that type III interferons might be important for the immune response to other pathogens as well. In a cohort of 914 Malian children, we analyzed episodes of malaria, gastrointestinal and respiratory infections using information for 30,626 clinic visits from birth through up to 5 years of follow-up. Genetic polymorphismsIFNL4-rs368234815 andIFNL3-rs4803217 that functionally affect type III interferons were genotyped with TaqMan assays. For both genetic variants and each infection, we evaluated time-to-first episode and calculated odds ratios (ORs) for the risk of an infection episode during follow-up, controlling for relevant covariates. Compared to children with the rs368234815-TT/TT genotype (IFN-λ4-Null), each copy of the rs368234815-dG allele was associated with an earlier first episode of a gastrointestinal infection (p=0.003) and respiratory infection (p=0.045). The risk of experiencing an infection episode during the follow-up was also significantly increased with each copy of the rs368234815-dG allele – for gastrointestinal infections (OR=1.53, 95%CI (1.13-2.07), p=0.005) and malaria (OR=1.30, 95%CI (1.02-1.65), p=0.033).IFNL4-rs368234815 andIFNL3-rs4803217 were in moderate linkage disequilibrium in this population (r2=0.78), and all the associations for rs4803217 were weaker and lost significance after adjusting for rs368234815, implicating IFN-λ4 and not IFN-λ3 as the primary cause of these associations. We conclude that the ability to produce IFN-λ4 may have broad health-related implications by negatively affecting the immune response and clinical outcomes of several common infections.
Object selection by automatic spreading of top-down attentional signals in V1
Authors: Ekman Matthias , Roelfsema Pieter R. , de Lange Floris P. 
Published: February 2020

AbstractWhat is selected when attention is directed to a specific location of the visual field? Theories of object-based attention have suggested that when spatial attention is directed to part of an object, attention does not simply enhance the attended location but automatically spreads to enhance all locations that comprise the object. Here, we tested this hypothesis by reconstructing the distribution of attention from population neuronal activity patterns in V1 using functional magnetic resonance imaging (fMRI) and population-based receptive field mapping. We find that attention spreads from a spatially cued location to the underlying object – and enhances all spatial locations that comprise the object. Importantly, this spreading was also evident when the object was not task-relevant. These data suggest that attentional selection automatically operates at an object level, facilitating the reconstruction of coherent objects from fragmented representations in early visual cortex.
Automatic classification of constitutive and non-constitutive metabolites with gcProfileMakeR
Authors: Pérez-Sanz Fernando , Ruiz-Hernández Victoria , Terry Marta Isabel , Arce-Gallego Sara , Weiss Julia , Navarro Pedro J , Egea-Cortines Marcos 
Published: February 2020

AbstractData analysis in non-targeted metabolomics is extremely time consuming. Genetic factors and environmental cues affect the composition and quantity of present metabolites i.e. the constitutive and non-constitutive metabolites. We developed gcProfileMakeR, an R package that uses standard output files from GC-MS for automatic data analysis using CAS numbers. gcProfileMakeR produces three outputs: a core or constitutive metabolome, a second list of compounds with high quality matches that is non-constitutive and a third set of compounds with low quality matching to MS libraries. As a proof of concept, we defined the floral scent emission ofAntirrhinum majususing wild type plants, the floral identity mutantsdeficiensandcompactaas well as RNAi lines ofAmLHY. Loss of petal identity was accompanied by appearance of aldehydes typical of green leaf volatile profiles. Decreased levels ofAmLHYcaused a major increase in volatile complexity, and activated the synthesis of benzyl acetate, absent in WT. Furthermore, some volatiles emitted in a gated fashion in WT such as methyl 3,5-dimethoxybezoate or linalool became constitutive. Using sixteen volatiles of the constitutive profile, all genotypes were classified by Machine Learning with 0% error. gcProfileMakeR may thus help define core and pan-metabolomes. It enhances the quality of data reported in metabolomic profiles as text outputs rely on CAS numbers. This is especially important for FAIR data implementation.One sentence summarygcProfileMakeR allows the automatic annotation of the core metabolome and non-constitutive metabolites, increasing speed and accuracy of non-targeted metabolomics.
An original structural fold underlies the multitask P1, a silencing suppressor encoded by the Rice yellow mottle virus
Authors: Poignavent Vianney , Hoh François , Terral Guillaume , Yinshan Yang , Gillet François-Xavier , Kim Jeong-Hyeon , Allemand Frédéric , Lacombe Eric , Brugidou Christophe , Cianferani Sarah , Déméné Hélène , Vignols Florence 
Published: February 2020

ABSTRACTThe Rice Yellow Mottle sobemovirus (RYMV) belongs to the most damaging pathogens devastating rice fields in Africa. P1, a key protein for RYMV, was reported as a potent RNAi suppressor counteracting RNA silencing in plant reporter systems. Here we describe the complete 3D structure and dynamics of P1. Its N-terminal region contains ZnF1, a structural CCCC-type zinc finger strongly affine to zinc and a prominent short helix, rendering this region poorly amenable to structural changes. P1 C-terminal region contains ZnF2, an atypical HCHC-type ZnF that does not belong to any existing class of Zn finger proteins. ZnF2 appeared much less affine to zinc and more sensitive to oxidizing environments than ZnF1, and may serve as a sensor of plant redox status. The structure helped us to identify key residues essential for RYMV infectivity and spread in rice tissues through their participation in P1 oligomerization and folding. Altogether, our results provide the first complete structure of an antiviral silencing suppressor encoded by a virus infecting rice and highlight P1 structural and dynamical properties that may serve RYMV functions to infect and invade its host plant.