bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic analysis reflects an environmental alkalinization-coupled pH-dependent mechanism of regulating lignocellulases in Trichoderma guizhouense NJAU4742
Authors: Miao Youzhi , Chen Xing , Li Tuo , Zhu Han , Tang Siyu , Liu Dongyang , Shen Qirong 
Published: January 2020

AbstractBackgroundFilamentous fungi have the ability to efficiently decompose plant biomass, and thus are widely used in the biofuel and bioprocess industries. In process, ambient pH has been reported to strongly affect the performance of the applied functional filamentous fungi. In this study,Trichoderma guizhouenseNJAU4742 was investigated under the fermentation of rice straw at different initial pH values for a detailed study.ResultsThe results showed that NJAU4742 strain could tolerate ambient pH values ranging from 3.0 to 9.0, but had significantly higher growth speed and extracellular enzyme activities under acidic conditions. At low ambient pH (< 4), NJAU4742 strain achieved rapid degradation of rice straw by elevating the ambient pH to an optimal range through environmental alkalinization. Further proteomic analysis identified a total of 1139 intracellular and extracellular proteins during the solid-state fermentation processes, including the quantified 190 carbohydrate-active enzymes (CAZymes) responsible for rice straw degradation, such as 19 cellulases, 47 hemicellulases and 11 chitinases. Meanwhile, the analysis results clearly showed that the secreted lignocellulases had a synergistic trend in distribution according to the ambient pH, and thus led to a pH-dependent classification of lignocellulases inT. guizhouenseNJAU4742.ConclusionsMost functional lignocellulases were found to be differently regulated by the ambient pH inT. guizhouenseNJAU4742, which had the ability of speeding up biomass degradation by elevating the ambient pH through environmental alkalinization. These findings contribute to the theoretical basis for the biodegradation of plant biomass by filamentous fungi in the biofuel and bioprocess industries.
Essential items for reporting of scaling studies of health interventions (SUCCEED): protocol for a systematic review and Delphi process
Authors: Gogovor Amédé , Zomahoun Hervé Tchala Vignon , Ben Charif Ali , McLean Robert K. D. , Moher David , Milat Andrew , Wolfenden Luke , Prévost Karina , Aubin Emmanuelle , Rochon Paula , Ekanmian Giraud , Sawadogo Jasmine , Rheault Nathalie , Légaré France 
Published: January 2020

Abstract
Background
The lack of a reporting guideline for scaling of evidence-based practices (EBPs) studies has prompted the registration of the Standards for reporting studies assessing the impact of scaling strategies of EBPs (SUCCEED) with EQUATOR Network. The development of SUCCEED will be guided by the following main steps recommended for developing health research reporting guidelines.

Methods
Executive Committee. We established a committee composed of members of the core research team and of an advisory group.
Systematic review. The protocol was registered with the Open Science Framework on 29 November 2019 (https://osf.io/vcwfx/). We will include reporting guidelines or other reports that may include items relevant to studies assessing the impact of scaling strategies. We will search the following electronic databases: EMBASE, PsycINFO, Cochrane Library, CINAHL, Web of Science, from inception. In addition, we will systematically search websites of EQUATOR and other relevant organizations. Experts in the field of reporting guidelines will also be contacted. Study selection and data extraction will be conducted independently by two reviewers. A narrative analysis will be conducted to compile a list of items for the Delphi exercise. Consensus process. We will invite panelists with expertise in: development of relevant reporting guidelines, methodologists, content experts, patient/member of the public, implementers, journal editors, and funders. We anticipated that three rounds of web-based Delphi consensus will be needed for an acceptable degree of agreement. We will use a 9-point scale (1 = extremely irrelevant to 9 = extremely relevant). Participants’ response will be categorized as irrelevant (1–3), equivocal (4–6) and relevant (7–9). For each item, the consensus is reached if at least 80% of the participants’ votes fall within the same category. The list of items from the final round will be discussed at face-to-face consensus meeting. Guideline validation. Participants will be authors of scaling studies. We will collect quantitative (questionnaire) and qualitative (semi-structured interview) data. Descriptive analyses will be conducted on quantitative data and constant comparative techniques on qualitative data.

Discussion
Essential items for reporting scaling studies will contribute to better reporting of scaling studies and facilitate the transparency and scaling of evidence-based health interventions.
Timing of Adopting a Flexible Manufacturing System and Product Differentiation
Authors: Sun Chia-Hung 
Published: January 2020

Abstract
Based on a circular product-space model with continuous time, we investigate a dynamic game in which each firm decides whether and when to adopt a flexible manufacturing system (FMS) at the beginning of the game and then chooses its quantity supplied at each time. We show that the equilibrium outcomes may be either joint adoption at the beginning of the game or sequential adoption, depending on the range of an initial adoption cost. For a given basic product, we find that a larger product differentiation decelerates the adoption of FMS. We also investigate competition behavior when the decisions on product locations are made endogenously and conduct welfare analysis, showing that there is market failure in the adoption timing choices.
An update on the seaweed resources of Japan
Authors: Tanaka Kouki , Ohno Masao , Largo Danilo B. 
Published: January 2020

Abstract
Natural seaweed beds, which play an important role for coastal ecosystems and marine fisheries, have been changing drastically in Japan. Interventions, such as decreasing the over-grazing by sea urchins, are being undertaken to maintain natural seaweed beds. Japan has at least 15 seaweed genera of economic importance utilized either directly as human food, as ingredients in food, or as raw materials for extraction of chemical compounds for various applications. Most species are produced through various cultivation methods that usually start in a land-based facility and are completed when seaweeds are transferred for open-sea cultivation. Cultivation, as well as harvesting, from natural populations, has sustained the Japanese seaweed industry for many years, but recently a downfall in seaweed production has been experienced in some areas for certain species due to warming oceans. Efforts to improve production have led to the development of forced cultivation techniques to reduce the cultivation period for Saccharina japonica while hybridization of different morphological forms of Undaria pinnatifida has resulted in the production of so-called “southern” and “northern types” that have desirable shapes for harvesting and processing. The future of the seaweed industry in Japan is geared towards producing high-grade products from seaweeds grown in land-based tanks using deep-ocean water.
Hyphenated mass spectrometry techniques for assessing medication adherence: advantages, challenges, clinical applications and future perspectives
Authors: Tanna Sangeeta , Ogwu John , Lawson Graham 
Published: January 2020

Abstract
Nonadherence to prescribed pharmacotherapy is an understated public health problem globally and is costing many patients their chance to return to good health and healthcare systems billions. Clinicians need an accurate assessment of adherence to medications to aid the clinical decision-making process in the event of poor patient progress and to maximise the patient health outcomes from the drug therapies prescribed. An overview of indirect and direct methods used to measure medication adherence is presented, highlighting the potential for accurate measuring of drugs in biological samples using hyphenated mass spectrometry (MS) techniques to provide healthcare professionals with a reliable evidence base for clinical decision making. In this review we summarise published applications of hyphenated MS techniques for a diverse range of clinical areas demonstrating the rise in the use of such direct methods for assessing medication adherence. Although liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods using plasma, serum and urine samples are the most popular, in recent years increased attention has been given to liquid chromatography high-resolution mass spectrometry (LC-HRMS) methods and alternative biosample matrices including hair, saliva and blood microsamples. The advantages and challenges of using hyphenated MS techniques to address this healthcare problem are also discussed alongside future perspectives.
Simultaneous quantitation of five triazole anti-fungal agents by paper spray-mass spectrometry
Authors: Skaggs Christine L. , Ren Greta J. , Elgierari El Taher M. , Sturmer Lillian R. , Shi Run Z. , Manicke Nicholas E. , Kirkpatrick Lindsey M. 
Published: January 2020

Abstract

Background
Invasive fungal disease is a life-threatening condition that can be challenging to treat due to pathogen resistance, drug toxicity, and therapeutic failure secondary to suboptimal drug concentrations. Frequent therapeutic drug monitoring (TDM) is required for some anti-fungal agents to overcome these issues. Unfortunately, TDM at the institutional level is difficult, and samples are often sent to a commercial reference laboratory for analysis. To address this gap, the first paper spray-mass spectrometry assay for the simultaneous quantitation of five triazoles was developed.

Methods
Calibration curves for fluconazole, posaconazole, itraconazole, hydroxyitraconazole, and voriconazole were created utilizing plasma-based calibrants and four stable isotopic internal standards. No sample preparation was needed. Plasma samples were spotted on a paper substrate in pre-manufactured plastic cartridges, and the dried plasma spots were analyzed directly utilizing paper spray-mass spectrometry (paper spray MS/MS). All experiments were performed on a Thermo Scientific TSQ Vantage triple quadrupole mass spectrometer.

Results
The calibration curves for the five anti-fungal agents showed good linearity (R2 = 0.98–1.00). The measured assay ranges (lower limit of quantification [LLOQ]–upper limit of quantitation [ULOQ]) for fluconazole, posaconazole, itraconazole, hydroxyitraconazole, and voriconazole were 0.5–50 μg/mL, 0.1–10 μg/mL, 0.1–10 μg/mL, 0.1–10 μg/mL, and 0.1–10 μg/mL, respectively. The inter- and intra-day accuracy and precision were less than 25% over the respective ranges.

Conclusions
We developed the first rapid paper spray-MS/MS assay for simultaneous quantitation of five triazole anti-fungal agents in plasma. The method may be a powerful tool for near-point-of-care TDM aimed at improving patient care by reducing the turnaround time and for use in clinical research.
Assessment of the glomerular filtration rate (GFR) in kidney transplant recipients using Bayesian estimation of the iohexol clearance
Authors: Riff Camille , Besombes Joevin , Gatault Philippe , Barbet Christelle , Büchler Matthias , Blasco Hélène , Halimi Jean-Michel , Barin-Le Guellec Chantal , Benz-de Bretagne Isabelle 
Published: January 2020

Abstract

Background
Plasma iohexol clearance (CLiohexol) is a reference technique for glomerular filtration rate (GFR) determination. In routine practice, CLiohexol is calculated using one of several formulas, which have never been evaluated in kidney transplant recipients. We aimed to model iohexol pharmacokinetics in this population, evaluate the predictive performance of three simplified formulas and evaluate whether a Bayesian algorithm improves CLiohexol estimation.

Methods
After administration of iohexol, six blood samples were drawn from 151 patients at various time points. The dataset was split into two groups, one to develop the population pharmacokinetic (POPPK) model (n = 103) and the other (n = 48) to estimate the predictive performances of the various GFR estimation methods. GFR reference values (GFRref) in the validation dataset were obtained by non-compartmental pharmacokinetic (PK) analysis. Predictive performances of each method were evaluated in terms of bias (ME), imprecision (root mean square error [RMSE]) and number of predictions out of the ±10% or 15% error interval around the GFRref.

Results
A two-compartment model best fitted the data. The Bayesian estimator with samples drawn at 30, 120 and 270 min allowed accurate prediction of GFRref (ME = 0.47%, RMSE = 3.42%), as did the Brøchner-Mortensen (BM) formula (ME = − 0.0425%, RMSE = 3.40%). With both methods, none of the CL estimates were outside the ±15% interval and only 2.4% were outside the ±10% for the BM formula (and none for the Bayesian estimator). In patients with GFR ≤30 mL/min/1.73 m2, the BM formula performed very well, while the Bayesian method could not be evaluated in depth due to too small a number of patients with adequate sampling times.

Conclusions
GFR can be estimated with acceptable accuracy in kidney transplant patients using the BM formula, but also using a Bayesian algorithm.
Trueness evaluation and verification of inter-assay agreement of serum folate measuring systems
Authors: Braga Federica , Frusciante Erika , Ferraro Simona , Panteghini Mauro 
Published: January 2020

Abstract

Background
Definitive data to establish if the use of the WHO International Standard (IS) 03/178 as a common calibrator of commercial measuring systems (MSs) has improved the harmonization of serum total folate (tFOL) measurements to a clinically suitable level are lacking. Here, we report the results of an intercomparison study aimed to verify if the current inter-assay variability is acceptable for clinical application of tFOL testing.

Methods
After confirming their commutability, the IS 03/178 and National Institute for Standards and Technology SRM 3949 L1 were used for evaluating the correctness of traceability implementation by manufacturers and the MSs trueness, respectively. The inter-assay agreement was verified using 20 patient pools. The measurement uncertainty (U) of tFOL measurements on clinical samples was also estimated. An outcome-based model for defining desirable performance specifications for bias and imprecision for serum tFOL measurements was applied.

Results
The majority of evaluated MSs overestimated the WHO IS value of +5% or more with the risk to produce an unacceptably high number of false-negative results in clinical practice. The mean inter-assay CV on all pools and on those with tFOL values >3.0 μg/L (n = 15) was 12.5% and 7.1%, respectively. In neither case the goal of 3.0% was fulfilled. The residual bias resulted in an excessive U of tFOL measurement on clinical samples.

Conclusions
The implementation of traceability of tFOL MSs to the WHO IS 03/178 is currently inadequate, resulting in an inter-assay variability that does not permit the use of a common threshold for detecting folate deficiency.
Reference-mean-centered statistical quality control
Authors: Yago Martín , Pla Carolina 
Published: January 2020

Abstract

Background
Statistical quality control (SQC) procedures generally use rejection limits centered on the stable mean of the results obtained for a control material by the analyzing instrument. However, for instruments with significant bias, re-centering the limits on a different value could improve the control procedures from the viewpoint of patient safety.

Methods
A statistical model was used to assess the effect of shifting the rejection limits of the control procedure relative to the instrument mean on the number of erroneous results reported as a result of an increase in the systematic error of the measurement procedure due to an out-of-control condition. The behaviors of control procedures of type 1
ks
(k = 2, 2.5, 3) were studied when applied to analytical processes with different capabilities (σ = 3, 4, 6).

Results
For measuring instruments with bias, shifting the rejection limits in the direction opposite to the bias improves the ability of the quality control procedure to limit the risk posed to patients in a systematic out-of-control condition. The maximum benefit is obtained when the displacement is equal to the bias of the instrument, that is, when the rejection limits are centered on the reference mean of the control material. The strategy is sensitive to error in estimating the bias. Shifting the limits more than the instrument’s bias disproportionately increases the risk to patients. This effect should be considered in SQC planning for systems running the same test on multiple instruments.

Conclusions
Centering the control rule on the reference mean is a potentially useful strategy for SQC planning based on risk management for measuring instruments with significant and stable uncorrected bias. Low uncertainty in estimating bias is necessary for this approach not to be counterproductive.
Complementary methods for characterization of the corrosion products on the surface of Ag60Cu26Zn14 and Ag58.5Cu31.5Pd10 brazing alloys
Authors: Dimitrijević Stevan P. , Vurdelja Borislava D. , Dimitrijević Silvana B. , Veljković Filip M. , Kamberović Željko J. , Veličković Suzana R. 
Published: January 2020

AbstractCorrosion products formed on the surface of two silver brazing alloys after the potentiostatic polarization in 3.5% sodium chloride solution were characterized by the standard methods such as, the X-ray diffraction, micro-Raman spectroscopy, and scanning electron microscopy with energy-dispersive spectroscopy. This paper presents the results of a laser desorption/ionization mass spectrometry (LDI MS) analysis as a new approach to the characterization of corrosion products. The potential of the anodic polarization was 0.5 V versus saturated calomel electrode, and the process duration was 300 s. The corrosion layers on both investigated alloys were similar in composition with cuprous chloride and silver chloride as the main components and had strong indications of cuprous oxide formation. The major difference between these two layers was the existence of zinc hydroxychloride as the corrosion product of Ag-Cu-Zn alloy. Palladium compounds were not found in the case of Ag-Cu-Pd alloy. The results of different methods have shown a good consistency. Complementarity between the used methods was useful in the interpretation of the results for each used method. This study has demonstrated that LDI MS can be used as an efficient additional method together with the traditional ones.
Lack of Statutory Foundation, Vagueness, and Violation of the Rule of Lenity of California Second Degree Felony Murder
Authors: Grosso Sira 
Published: January 2020

Abstract
Restrictions recently imposed by law on the application of felony murder in California
Senate Bill n. 1437 amending Sections 188 and 189 of, and adding Section 1170.95 to, the Penal Code.
and a judgment of a split California Court of Appeal rejecting the claim that the second-degree felony murder provision is unconstitutionally vague
Cal. 4th App. Dist. April 30, 2019, see note 74.
offer the occasion to analyze the Californian second degree murder. Second degree Felony murder in California, which is not spelled out by statute, relies on the jurisprudential construction of an “inherently dangerous felony”. According to the California Supreme Court, this criterion was conceived to accompany its overall aim of deterrence as well as function as a limitation on the application of felony murder itself. The purpose of this article is to highlight how the jurisprudential reconstruction of “inherently dangerous felonies” does not present a suitable criterion for determining whether second degree murder has taken place. While calling into play the goal of deterrence may bring forth paradoxical results, the “inherently dangerous felony,” upon which second degree felony murder relies, represents a double-edged sword. In fact, this article argues that it expands, rather than restricts, the application of felony murder, also posing several constitutional concerns. It follows that, since the “inherently dangerous” category acts as the essential base upon which second degree felony murder stands, the “crumbling” of the one should lead to the fall of the other.
The effect of curcuma (Curcuma xanthorrizha roxb.) extract as an adjuvant of captopril therapy on cardiac histopathology of male mice (Mus musculus) with hypertension
Authors: Hijriani Nursela , Yusetyani Lilik , Hasmono Didik 
Published: January 2020

Abstract

Background
Hypertension is a cardiovascular disease which has become a major health problem in Indonesia. Left ventricle hypertrophy is one of the cardiac complications of hypertension that is characterized by thickening of the left ventricle and increasing the mass of cardiac muscle.

Methods
This study was an experimental study with a posttest group design. Twenty-four mice were divided into four groups. The normal group was given distilled water, the negative control group was given L-NAME 1.75 mg/25 g BW/day, the positive control group was given L-NAME 1.75 mg/25 g BW/day + captopril 0.04875 mg/30 g BW/day, and the adjuvant captopril group was given L-NAME 1.75 mg/25 g BW/day + captopril 0.04875 mg/30 g BW/day + curcuma extract 31.25 mg/30 g BW for 30 days.

Results
The results of one-way analysis of variance (ANOVA) test analysis on the adjuvant treatment of the captopril group revealed no significant effect on cardiac muscle mass (p > 0.05), while the thickness of the left ventricle was significant (p < 0.05).

Conclusions
Captopril-Curcuma group resulted in a decrease of cardiac muscle and the thickness of the left ventricle in male mice with hypertension.
Analysis of the use and cost of stress ulcer prophylaxis for surgical inpatients
Authors: Wijaya Dhani , Padolo Elfri , Ardianto Chrismawan , Sumarno  , Matulatan Fendy , Alderman Chris , Suharjono  
Published: January 2020

Abstract

Background
Stress ulcer is a superficial and asymptomatic lesion and causes bleeding. As many as 50% of death cases are reported as the result of stress ulcer bleeding. Stress ulcer prophylaxis (SUP) is a drug used to prevent gastrointestinal tract injuries due to stress ulcers. The inappropriate use of SUP drugs can cause adverse drug reactions, and thus SUP drugs are only given to patients in accordance with guidelines in order to avoid the overuse of SUP drugs. The aim of this present study is to analyse the suitability of SUP drug usage based on the criteria from the American Society of Health-System Pharmacists (ASHP) and the drug costs of SUP overuse.

Methods
An observational descriptive study was conducted from April 24, 2019, to May 17, 2019, in the inpatient surgical ward of Dr. Soetomo General Hospital. Data were obtained from patient medical health records.

Results
One hundred fifty-two patients used 1404 SUP drugs. Approximately 48% of usage did not suit the ASHP criteria and was considered as medication overuse. The cost of excessive SUP usage during the study period was more than US $65, which is 30.08% of the total drug cost of prescribed stress ulcer drugs.

Conclusions
The present study suggests that the relatively high excessive drug costs for SUP show a need for monitoring of the application of SUP therapy guidelines.