bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association between dietary acid load and the migraine odds
Authors: Mousavi Maryam , Jahromi Soodeh Razeghi , Togha Mansoureh , Ghorbani Zeinab , Hekmatdoost Azita , Rafiee Pegah , Torkan Boshra , Shirani Pedram , Ansari Hossein , Karami Ahmadreza , Nourmohammadi Morvarid , Khorsha Faezeh 
Published: August 2020

Abstract
Background: According to previous evidence, it has been shown that high acidic load in human body could affect inflammatory factors and the nitric oxide pathway. On the other hand, these factors are thought to play a role in initiation of migraine attacks. Therefore, we aimed to explore the association between dietary acid load and the odds of migraine in a case-control study.Methods: The migraine group (n=514, diagnosed according to the ICHDIII criteria) was recruited from a tertiary headache clinic. The controls consisted of 582 sex-matched healthy volunteers who were randomly selected from the general population. For dietary intake assessments, we used a validated 168-item semiquantitative food frequency questionnaire (FFQ). Using three different measures, such as potential renal acid load (PRAL), net endogenous acid production (NEAP), and protein /potassium ratio for estimation of the dietary acid load.Results: According to multivariable logistic regression analysis, in comparison with the lowest tertile, highest tertile of dietary acid load measures including PRAL (OR=7.208, 95% CI 3.33– 15.55), NEAP (OR=4.108, 95% CI 1.924– 8.774), protein/potassium ratio (OR=4.127, 95% CI 1.933– 8.814) were shown to significantly increase odds of migraine (P for trend= <0.001).Conclusions: In this study, it was found that high dietary acid load may be associated with higher odds of migraine. Consequently, restricting dietary acid load could be beneficial in reducing the odds of developing migraine in susceptible subjects.
Global Metabolomic and Lipidomic Analysis Reveals the Potential Mechanisms of Hemolysis Effect of Ophiopogonin D and Ophiopogonin D’ in vivo
Authors: Huan-Hua Xu , Jiang Zhen-Hong , Huang Cong-Shu , Sun Yu-Ting , Xu Long-Long , Tang Xiang-Ling , Tan Hong-Ling , Ma Zeng-Chun , Gao Yue 
Published: August 2020

Abstract
Background

OPD and OPD’ are the two main active monomers of Ophiopogon japonicus in Shenmai injection, being isomers of each other with the same chemical formula and similar chemical structure. According to common sense, they are supposed to have similar pharmacological activities, but the actual situation is exactly the opposite. The difference of distinct hemolytic behavior between OPD and OPD’ in vivo and in vitro was discovered and reported by our group for the first time. In the hemolysis experiment in vitro, only OPD’ showed hemolysis reaction, while in vivo, both OPD and OPD’ revealed hemolysis reaction. The primary cause of hemolysis in vitro has been confirmed to be related to the difference between physical and chemical properties of OPD and OPD’, but the mechanism of hemolysis in vivo is still unclear, especially the existing research are still difficult to explain why OPD shows the inconsistent hemolysis behavior in vivo and in vitro. However, the detection of hemolysis of OPD and OPD’ in vivo is of great practical significance in response to the increase of adverse events of Shenmai injection.

Methods

Aiming at the phenomenon of hemolysis in vivo, this paper adopted untargeted metabolomics and lipidomics technology to preliminarily explore the changes of plasma metabolites and lipids of OPD and OPD’ in vivo during the occurrence of hemolysis. Metabolomics and lipidomics analysis was performed on ultra-high performance liquid chromatography (UPLC) system tandem with different mass spectrometer and different columns respectively. Multivariate statistical approaches such as principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA) were applied to screen the differential metabolites and lipids.

Results

Both OPD and OPD’ groups experienced hemolysis, Changes in endogenous differential metabolites and differential lipids, enrichment of differential metabolic pathways, and correlation analysis of differential metabolites and lipids all indicated that the causes of hemolysis by OPD and OPD’ were closely related to the interference of phospholipid metabolism.

Conclusions

This study provided a comprehensive description of metabolomics and lipidomic changes between OPD and OPD’ treated rats, which also provided scientific guidance for the subsequent mechanism research, and the underlying mechanism require further research.
A comparison of the chemical compounds in single- and co-boiled Zhi-Zi-Da-Huang decoctions with UPLC-qTOF-MS/MS and UPLC-DAD
Authors: Xia Mengyu , Zhang Xue , Wu Hongwei , Wang Yun , Zhang Cun 
Published: August 2020

Abstract
Background

Zhi-Zi-Da-Huang decoction (ZZDHD) is a famous Traditional Chinese Medicine decoction due to its therapeutic effects on clinical hepatobiliary disorders. ZZDHD is composed of Gardeniae Fructus, Rhei Radix et Rhizoma, Fructus Aurantii Immaturus, and Sojae Semen Praeparatum. With the development of current technology, dispensing granules have been widely used for convenience. However, limited research has been conducted to determine differences in the chemical compounds between dispensing granules and traditional decoction.
Methods

A strategy based on UPLC-qTOF-MS/MS and UPLC-DAD was established to quantitatively and qualitatively analyze the chemical compounds present in single- and co-boiled ZZDHD. First, we utilized UPLC-qTOF-MS/MS to identify the compounds in single- and co-boiled ZZDHD. Then, 15 compounds were quantitatively analyzed in ZZDHD by UPLC-DAD. Finally, fingerprint and chemometric analyses were adopted to evaluate the difference between single- and co-boiled ZZDHD.
Results

One hundred and forty-seven compounds were authenticated or determined according to their retention behaviors, mass mensuration, and characteristic fragment ions. In addition, 15 analytes of single- and co-boiled ZZDHD indicated a significant difference in the content of many compounds, including geniposide (G2), emodin, chrysophanol, daidzin, and crocin-I. The similarities of single and co-boiled ZZDHD were higher than 0.99, as evaluated by fingerprinting; 30 common peaks were evaluated by the chemometric analysis, which found 13 important variable values (VIP > 1) including C3, A3, crocetin, daidzin, C5, physcion, G2, genipin-1-O-β-D-gentiobioside (G1), chrysophanol, emodin, A2, A5, and C6.
Conclusions

This study provided an experimental basis for explaining the rationality of single-and co-boiled ZZDHD, and a study model for comparing the chemical substances between traditional decoctions and dispensing granules.
Healthcare providers’ practice and attitude towards abortion service provision in Gulu city, Northern Uganda
Authors: Pebalo Francis Pebolo , Grace Auma Anna , Henry Obol James 
Published: August 2020

Abstract
Background: Unsafe abortion is a growing public health problem concern globally despite being preventable. The World Health Organization estimates that about 20 million pregnancies results into unsafe abortion globally. The proportion of unsafe abortion has been increasing with developing countries contributing about 97% of unsafe abortions cases including Uganda. Unsafe abortion is among the four leading causes of pregnancy related mortality, injuries, and disability globally. The restrictive abortion laws and religious situation make abortion a highly controversial social issue in Uganda leading to high stigma in the society, and a majority of the healthcare providers are reluctant to perform an abortion even if indicated for fear of possible legal consequences.Methods: We conducted survey among health worker about knowledge of complications, practice, and attitudes of induced abortion between September and November 2019 among 252 healthcare providers in Gulu Municipality, Northern Uganda. Multivariate analysis showed associations between healthcare providers' characteristics and adequate knowledge about abortion complications. Ordinary least square regression analysis found associations between providers' characteristics and their intention for general support, generally not in support, conditional support for abortion provision, as well as their personal attitudes and beliefs against or towards abortion provision.Results: The mean attitudinal score for generally in support, generally not in support, conditional in support, personal attitude, and beliefs against and toward abortion provision were 2.80, 2.71, 2.86, 3.239, and 3.35 respectively. Participants who were married and practice Anglican religion were more likely to have good knowledge of abortion complications, p-values 0.035, and 0.042 respectively, meanwhile participants who were employed in faith-based facilities were more likely to have poor knowledge of abortion complications p-value 0.002.Conclusion: Ministry of Health and stakeholders need to provide training of health workers to improve quality of abortion services. Medical training institutions should ensure that students understand the laws and responsibilities that govern their professional actions with respect to abortion care regardless of their personal views, beliefs, and attitudes.
Overlapping syndrome mimicking infectious meningoencephalitis in a patient with MOG and GFAP IgG
Authors: Ji Su-qiong , Liu Chen-chen , Bu Bi-tao 
Published: August 2020

Abstract
Background
The presence of CNS overlapping autoimmune syndrome is not uncommon, but only one case of overlapping syndrome with coexistence of MOG-IgG and GFAP-IgG had been reported. This is the first reported case of these double antibodies positive presenting as clinical meningoencephalitis.
Case presentation:
A 23-year-old woman presented with transient convulsions,loss of consciousness, persistent fever, headache and vomiting. The cerebrospinal fluid (CSF) analysis revealed elevated cellularity, Magnetic resonance imaging (MRI) showed diffuse leptomeningeal enhancement. She remained fever and headache with antiviral and antibiotic treatment for two weeks, then was treated with empirical anti-tuberculosis treatment and oral prednisolone therapy. She followed up at 3 months from presentation with symptoms improved and normal CSF analysis. 3-month follow-up MRI performed asymmetric lesions in the cerebellum, corona radiata, and white matter with enhancement. Anti-tuberculosis treatment was continued and steroid was discontinued. After She stopped taking the prednisolone, interrupted headache gradually appeared. MRI at 4 months after presentation revealed partial reduced extent of lesions, but enlarged areas in left cerebellum and right parietal white matter, as well as a new lesion in the region of the right ependyma with linearly enhancement. Screening for anti-myelin oligodendrocyte glycoprotein (MOG) antibody and anti-glial fibrillary acidic protein (GFAP) antibody were positive in CSF by transfected cell-based assay. She was diagnosed with overlapping syndrome of MOG‑IgG‑associated disease and GFAP astrocytopathy and received steroid pulse therapy (methylprednisolone 1 g for 5 days) followed by a gradual tapering of oral prednisolone, as well as addition of immunosuppressant (tacrolimus, 3 mg per day). 6 months after the patient’s initial presentation, no symptom was found, MRI showed the lesions had obviously diminished and no enhancement was found.
Conclusions
To our knowledge, this is the first reported case of overlapping syndrome with coexistence of MOG-IgG and GFAP-IgG presenting as clinical meningoencephalitis. The early screening of autoantibodies against CNS antigens was of great importance for the patient suspected of intracranial infection to make the definite diagnosis.
An observational study of balance and proprioception function in patients with spinocerebellar ataxias type 3
Authors: Liu Xia-Hua , Li Ying , Xu Hao-Ling , Sikandar Arif , Lin Wei-Hong , Wang Ning , Ni Jun , Chen Wan-Jin , Gan Shi-Rui , Wang Zhi-Yong 
Published: August 2020

Abstract
BackgroundPostural instability is one of the most disabling features of spinocerebellar ataxias type 3 (SCA3) and often leads to falls that reduce mobility and functional capacity. This study aimed to quantitatively analyse static and dynamic balance and proprioception function on postural control in patients with SCA3 using the Pro-kin system and optimise rehabilitation programmes for them.MethodsEight-one clinically diagnosed SCA3 patients (38 women, 43 men; aged 39.00 ± 9.66) and 62 healthy controls were studied and evaluated using the Pro-kin system (PK254P, Tecnobody S.r.l, Dalmine, Italy). The measurements included (1) a static balance test in two visual feedback conditions: eyes open (EO) and eyes closed (EC); (2) a dynamic balance test measuring limits of stability (LOS); and (3) a proprioception function test to obtain proprioceptive measurements on a multiaxial balance evaluator for both right and left lower limbs.ResultsCompared to controls, SCA3 patients showed significantly higher values of all static balance outcome variables with eyes open and eyes closed, implying postural instability. SCA3 patients showed significantly higher values in the standard deviation of body sway along the medio-lateral (ML) axis and in the velocity of body sway along the anterior-posterior (AP) axis. The overall scores and the scores for all eight LOS components were significantly lower in the SCA3 patients than in the controls. The mean values of AP index (API), ML index (MLI), Stability index (SI) and average trace error (ATE) were significantly greater in SCA3 patients compared to HC subjects, while API showed a trend toward higher values.ConclusionsSCA3 patients have a significant postural control disorder, and are likely to fall on the AP plane and prefer performing postural adjustments in the ML direction; a decreased proprioception function in the knee and ankle is also evident. Visual cues and proprioception should be emphasized in balance rehabilitation training. Attention should also be paid to improve muscle strength and range of motion.Trial registrationThe Chinese clinical test registration center. ChiCTR1800020133. Registered 15 december 2018 - Retrospectively registered, http://www.chictr.org.cn/showprojen.aspx?proj=33950
Prevalence of Non-Communicable Diseases and Associated Medication Use Among Syrian Refugees in Lebanon: An Analysis of Country-Wide Data from the Sijilli Electronic Health Records Database
Authors: Saleh Shadi , Abdouni Lina , Dimassi Hani , Nabulsi Dana , Harb Ranime , Jammoul Zeinab , Hachach Noha , Arnaout Nour El 
Published: August 2020

Abstract
Background

Globally, the number of forcibly displaced individuals has reached 70.8 million. Lebanon, a middle income country, hosts the highest number of refugees per capita worldwide. The majority of refugees are Syrians who have fled the Syrian war that started in 2011. The migration journey exposes refugees to increased susceptibility to a wide range of medical issues including non-communicable diseases (NCDs). This study aims to determine the prevalence of NCDs among adult Syrian refugees in Lebanon, with a focus on hypertension, diabetes, cardiovascular diseases (CVD) and cancer. The study also aims to explore factors potentially related to the prevalence figures and understand the medication use associated with these morbidities.
Methods

This study is a secondary analysis of de-identified data from the “Sijilli Electronic Health Records for Refugees” Database comprising data on 10,082 Syrian refugees from across informal tented settlements located all over Lebanon. A total of 3,255 records of Syrian refugees aged above 18 years old and reporting having at least one condition of the following were included in the analysis: hypertension, diabetes, Cardiovascular diseases or cancer. Pearson’s Chi-square, independent t-test, and multivariate logistic regressions were used for data analysis.
Results

Hypertension was the most prevalent (10.0%) NCD among refugees, and a higher age was associated with higher NCDs prevalence. A strong linkage has been reported between smoking status and alcohol intake, and increased risk for NCDs. Study findings also revealed that the highest prevalence of hypertension, diabetes and CVDs was observed among refugees originating from Idlib, Aleppo and Homs. An association between adherence to medication and location of diagnosis was noted, with females who were diagnosed before moving to Lebanon being more likely to take corresponding medications compared to those diagnosed in Lebanon, with no difference reported among males.
Conclusions

Our findings suggest that efforts should be directed towards the employment of innovative low-cost approaches for NCD detection and control among refugees, with a focus on the importance of adherence to medication. Such efforts remain imperative to control the increasing burden of NCDs amongst refugee populations and improve equitable access to NCD services.
Circ_0026344 overexpression inhibits the migration, invasion, and enhances apoptosis of colorectal cancer cells by sponging miR-31
Authors: Jin Ye , Yu Lingli , Zhang Bin , Chen Yun , Liu Changfeng , Chen Zhi , Zhan Xiaobo , Wu Qi 
Published: August 2020

Abstract
Background: Circ_0026344 was reported to be associated with the metastasis of colorectal cancer (CRC). This study aimed to investigate the expression of circ_0026344 in CRC and the effect mechanisms of circ_0026344 on CRC.Methods: The expressions of circ_0026344 and miR-31 in clinical CRC tissues or CRC cell lines were analyzed by qPCR. The target of circ_0026344 was predicted and verified by CircInteractome and dual-luciferase reporter assays. The correlation between circ_0026344 and miR-31 expression was analyzed using Pearson analysis. After the CRC cells were overexpressed circ_0026344 or miR-31 or silenced circ_0026344, the viability, apoptosis, migration, and invasion of CRC cells were evaluated by CCK-8, flow cytometry, wound healing, and transwell. Also, the expressions of miR-31, Bcl-2, Bax, E-cadherin, and N-cadherin in the cells were detected by qPCR or Western blot. Results: Circ_0026344 was low-expressed in CRC tissues and cell lines. Circ_0026344 sponged miR-31 which was high-expressed in CRC tissues. The expression of circ_0026344 was negatively correlated to the expression of miR-31. The miR-31 expression could be down-regulated by circ_0026344 overexpression. Circ_0026344 overexpression inhibited the cell viability, migration, and invasion; and enhanced the apoptosis of CRC cells. Circ_0026344 overexpression decreased the expressions of Bcl-2 and N-cadherin and increased the expressions of Bax and E-cadherin in CRC cells. Circ_0026344 silencing and miR-31 overexpression had an opposite effect on CRC cells as circ_0026344 overexpression. Furthermore, miR-31 overexpression counteracted the effect of circ_0026344 overexpression.Conclusion: Circ_0026344 overexpression inhibited the migration, invasion, and enhanced apoptosis of CRC cells by sponging miR-31.
IL-33 / ST2 Signaling Promotes TF Expression by Regulating NF-κB Activation in Coronary Artery Endothelial Microparticles of Acute Myocardial Infarction
Authors: Yuan Yujuan , Cheng Hui , Tao Jing , Muyesai Nijiati 
Published: August 2020

Abstract
Background: Interleukin (IL)-33 was previously shown to induce angiogenesis and inflammatory activation of endothelial cells derived Microparticles（EMPs）. Tissue factor (TF) plays a central role in hemostasis and thrombosis. Objective: The aim of this study was to investigate the effect of IL-33 on TF release of EMPs, which may be a new link between inflammation and coagulation. Methods: The study analyzed the coronary blood of level of CD31+EMPs, TF protein and IL-33 protein in acute myocardial infarction(AMI) and stable coronary artery disease(SCAD) patients. Human coronary artery endothelial cells (HCAECs) were treated with IL-33 to obtain MPs. The TF activity of EMPs was tested by thermo fisher by adding the TF antibody. Furthermore, TF and TFPI protein were tested by ELISA. Finally, NF-κB inhibitor dimethyl fumarate (DMF) and soluble extracellular domain of ST2 coupled to the Fc fragment of human IgG1 (sST2) were added HCAECs, which were treated with IL-33, then the TF protein level also was tested by ELISA. Results: The AMI patients have higher level of CD31+EMPs, TF protein and IL-33 protein than the SCAD patients in coronary blood. In AMI patients (N=27) , the IL-33 protein positively correlated with CD31+EMPs (r = 0.794, p < 0.01). According to the ROC curve analysis, the areas under the curve (AUC) of CD31+EMPs, TF protein and IL-33 protein were 0.888, 0.962 and 0.778. In the cell culture, the TF activity and TF protein in ECs-derived MPs increased gradually with time of intervention by the treatment of IL-33. IL-33 binding to the ST2 receptor promoted TF expression by regulating NF-κB activation in ECs-derived MPs of HCAECs. Conclusion: Activated endothelial cells and their released MPs simultaneously express TF, which is a risk factor for cardiovascular disease.
Health-related quality of life of pharmacists in the pre-COVID-19 era
Authors: Kurnik-Łucka Magdalena , Pasieka Paweł , Górecka-Mazur Agnieszka , Rząsa-Duran Elżbieta , Pękala Jakub , Gil Krzysztof 
Published: August 2020

Abstract
Background

pharmacists remain on the frontline of public health around the globe and their performance directly impacts patients’ safety. So far, to our knowledge, no European study has been dedicated to their heath-related quality of life (HQoL). Therefore, the primarily aim of our study was to evaluate HQoL of Polish pharmacists utilizing the SF-36 health survey with regard to anthropometric and lifestyle-related variables.
Methods

a total sample screened consisted of 1412 respondents, yet 765 pharmacists (mean age 40, 86.3% females) finally participated in the study. HQoL was assessed with the Medical Outcomes Study 36-Item Short Form Health Survey (SF-36).
Results

the lowest median scores were noted for general health (GH, 50.0) and vitality (V, 60.0) domains. No gender differences regarding physical and mental summary scores were found. Significant difference of HQoL was found among the assessed age groups in several domains, especially physical functioning (PF) and GH (p < 0.001) scores, and especially in the group of 51-60-year-old-respondents. Correlations were found between PF (r=-0.29,p < 0.001), GH (r=-0.25,p < 0.001) and age as well PF (r=-0.27,p < 0.001), GH (r=-0.21,p < 0.001) and BMI. Self-assessed dietary habits were correlated with PF (r = 0.22,p < 0.001), mental health (r = 0.25,p < 0.001), GH (r = 0.27,p < 0.001) and V (r = 0.30, p < 0.001) scores.
Conclusions

our analysis indicates that pharmacists tend to have similar mental and physical burden according to SF-36, with age, BMI and dietary habits as predominant factors influencing their HQoL. The study presents unique values for future comparative analyses related, for instance, to the influence of the ongoing pandemic on HQoL of health-care providers.