bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the asymmetric effects of changes in crude oil prices on economic growth: New evidence from China's 31 provinces
Authors: Baek Jungho , Lu Guimin , Nam Soojoong 
Published: September 2020

AbstractAlthough previous literature has addressed an asymmetric response of economic growth to oil price movements in developed economies, less widely recognised is the possibility of such responses in developing economies. Thus, the primary thrust of the present article is to investigate the asymmetric effects of changes in crude oil prices on growth in a developing economy, particularly China. To examine this subject thoroughly, we first assume oil price symmetry and apply the method of the linear autoregressive distributed lag (ARDL) to China's 31 provinces. We discover that the price of crude oil has a significant effect on growth mostly in the short‐run. We then separate oil price hikes from oil price plunges and implement the method of the nonlinear ARDL (NARDL) to reveal that the price of crude oil has indeed an important role in affecting growth across China's 31 provinces in both the short‐ and long‐run. Further, there is evidence that fluctuations in oil prices appear to have asymmetric effects on growth for some provinces, though not all, in the short‐ and long‐run.
Fatal hemorrhage from a periumbilical wound: Stabbing or hemorrhage from a caput medusae?
Authors: Frigiolini Francesca , Lo Pinto Sara , Caputo Fiorella , Barranco Rosario , Fraternali Orcioni Giulio , Bonsignore Alessandro , Ventura Francesco 
Published: September 2020

AbstractVarices are the main clinical manifestation of portal hypertension, and their bleeding is the predominant cause of mortality from this condition. Periumbilical varices are known as “caput medusae.” Reports of their bleeding are rare, with only three fatal cases described in the literature. The antemortem diagnosis is relatively simple, while the postmortem diagnosis is more complex. This paper is the first report of fatal hemorrhage from a caput medusae for which the diagnosis was made postmortem, thanks to a complete diagnostic process including scene and circumstances, medical history, and autopsy with detailed histology. The circumstantial analysis showed the presence of a large amount of blood at the scene, blood which originated from a small abdominal wound; an analysis of the subject's clinical data reported that he was affected by portal hypertension. The autopsy revealed some dilated and convoluted veins in the subcutaneous tissue of the umbilical region; a fistula between these veins and the abdominal wound was detected. The histological study confirmed the presence of periumbilical varices, one of them ruptured and connected with the overlying skin. The cause of death was attributed to a massive hemorrhage generated by a periumbilical varix in a patient affected by portal hypertension.
Improved production of 2,3‐butanediol and isobutanol by engineering electron transport chain in Escherichia coli
Authors: Jung Hwi‐Min , Han Jae‐Ho , Oh Min‐Kyu 
Published: September 2020

SummaryThe electron transport chain (ETC) is one of the major energy generation pathways in microorganisms under aerobic condition. Higher yield of ATP can be achieved through oxidative phosphorylation with consumption of NADH than with substrate level phosphorylation. However, most value‐added metabolites are in an electrochemically reduced state, which requires reducing equivalent NADH as a cofactor. Therefore, optimal production of value‐added metabolites should be balanced with ETC in terms of energy production. In this study, we attempted to reduce the activity of ETC to secure availability of NADH. The ETC mutants exhibited poor growth rate and production of fermentative metabolites compared to parental strain. Introduction of heterologous pathways for synthesis of 2,3‐butanediol and isobutanol to ETC mutants resulted in increased titres and yields of the metabolites. ETC mutants yielded higher NADH/NAD+ ratio but similar ATP content than that by the parental strain. Furthermore, ETC mutants operated fermentative metabolism pathways independent of oxygen supply in large‐scale fermenter, resulting in increased yield and titre of 2,3‐butanediol. Thus, engineering of ETC is a useful metabolic engineering approach for production of reduced metabolites.
Downregulation of m6A reader YTHDC2 promotes tumor progression and predicts poor prognosis in non‐small cell lung cancer
Authors: Sun Shulei , Han Qiang , Liang Maoli , Zhang Qian , Zhang Jing , Cao Jie 
Published: September 2020

AbstractBackgroundm6A modification affects the pathological progress of many diseases by affecting RNA stability and translocation. YTHDC2, a m6A reader, is associated with multiple cancers; however, little is known of its role in non‐small cell lung cancer (NSCLC).MethodsThe GEPIA, Oncomine and GEO databases were analyzed to assess expression of YTHDC2 in NSCLC patients. Quantitative polymerase chain reaction, western blot and immunohistochemistry were used to detect YTHDC2 expression in different NSCLC cell lines (H1299, H460, H292 and A549) and patients. The effects of YTHDC2 on NSCLC cell lines (A549 and H1299) proliferation and migration were employed using CCK8 and transwell assays. The potential target RNAs of YTHDC2 were obtained from the POSTAR database. Functional enrichment analysis of YTHDC2 targeted RNAs was performed using the Metascape database.ResultsGEPIA, Oncomine and GEO databases showed low expression of YTHDC2 in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) patients. YTHDC2 expression was significantly decreased in different NSCLC cell lines and our clinical samples. Moreover, low expression of YTHDC2 was significantly associated with poor differentiation, lymph node metastasis, tumor size and stage. In addition, YTHDC2 could suppress the proliferation and migration ability of A549 and H1299 cell lines. Kaplan‐Meier Plotter database analysis revealed that patients with low level of YTHDC2 had a significantly poor prognosis. Finally, functional enrichment analysis of YTHDC2 targeted RNAs indicated several enriched pathways related to cancer.ConclusionsThese findings elucidate that YTHDC2 suppresses tumorigenesis in NSCLC, indicating that YTHDC2 may be a promising therapeutic target for NSCLC.Key pointsSignificant findings of the studyThis study demonstrated that the downregulation of YTHDC2 promotes tumor progression and predicts poor prognosis in non‐small cell lung cancer (NSCLC).What this study addsYTHDC2 might be a promising therapeutic target for non‐small cell lung cancer (NSCLC).
Limnological response to the Laacher See eruption (LSE) in an annually laminated Allerød sediment sequence from the Nahe palaeolake, northern Germany
Authors: Dreibrodt Stefan , Krüger Sascha , Weber Jan , Feeser Ingo 
Published: September 2020

This paper presents evidence for a limnological response to the Laacher See eruption (LSE) as detected in lake sediments from Nahe, northern Germany. The sediment section of the Allerød period dating to between 13 422 and 12 708 cal. a BP is preserved in annual laminations. Within this section, the LSE was identified as a cryptotephra layer (12 944±44 cal. a BP). Microfacies analysis, continuous high‐resolution geochemical measurements and pollen analyses enabled a high‐resolution reconstruction of environmental change. The older part of the Allerød (c. 13 422 to 12 943 cal. a BP) was characterized by relatively stable sedimentation conditions. Evidence for windier conditions dating to c. 13 160 to 13 080 cal. a BP probably reflects the Gerzensee oscillation. Pronounced changes of the lake sedimentation followed the LSE. Four unusually thick varves with increased amounts of allochthonous material indicate serious disturbance of the local environment immediately after the LSE, related to increased storminess and/or the occurrence of high intensity rainfall events. A pronounced reduction of biogenic silica accumulation for c. 60 years after the LSE could reflect a period of acidification. Indications of a simultaneous lake level increase until c. 60 years after the LSE are in line with the supposed reduced evapotranspiration associated with cooler conditions. About 120 years after the LSE, increased oxygen access at the lake bottom, allochthonous input and Cl fluxes point to an onset of increasingly stronger westerly winds, probably as a long‐term response to the LSE. This supports the idea of a southward shift of the mid‐latitude westerlies wind system within the interval between the LSE and the beginning of the Younger Dryas. The pace of the southwards shift of this wind system decreased from 10 km a−1 in the initial phase (40–120 years after LSE) to 6 km a−1 in the later phase (120–200 years after LSE).
Neogene to recent evolution of the Southern Gulf of Mexico basin: Tectonic controls on deep‐water sediment dispersal systems
Authors: Sickmann Zachary T. , Snedden John W. 
Published: September 2020

AbstractNewly available two‐dimensional (2D) and limited three‐dimensional (3D) reflection seismic data coupled with publicly available well and core data were used to generate the first comprehensive regional basin evolution model for the deep‐water Neogene to recent southern Gulf of Mexico (GoM). This evolution is presented in the context of contemporaneous onshore tectonic drivers and predecessor basin tectonic history. Dynamic uplift to the west in North America, transpression to the south and tectonically influenced salt tectonics to the east served to collapse the margins of the southern Gulf of Mexico Basin throughout the late Neogene and into the modern. This collapse and salt inflation served to shut off efficient sediment transport into the axial deep basin. Newly developed nearshore extensional accommodation along both the western margin of the basin and salt‐rooted orogenic structures to the south and east appear to have baffled coarser‐caliber sediment input from southern (Mexican) sediment sources into the deep Gulf of Mexico Basin beginning in the late Miocene. This sequence is recorded in the southern GoM basin by a transition from large early–mid Miocene submarine channel belts to late Miocene–recent sediment wave fields and mass transport deposits. Improved resolution of new 2D and 3D subsurface seismic reflection data shows that sediment waves of the southern Gulf of Mexico are supercritical bedforms, long wavelength antidunes and cyclic steps, not contourites. These supercritical bedforms are among the most spatially expansive and morphologically largest documented globally. The location and evolution of these bedforms appears closely tied to the tectonically driven history of margin collapse, salt inflation and the starving of the deep basin of coarse‐grained sediment. Cumulatively, the tectonic drivers that intuitively should have increased sediment flux into the deep southern Gulf of Mexico acted to ‘pinch off’ the basin, starving it of sediment supplied from southern sediment sources.
Synthesis and in vitro antileishmanial efficacy of novel quinazolinone derivatives
Authors: Prinsloo Izak F. , Zuma Nonkululeko H. , Aucamp Janine , N’Da David D. 
Published: September 2020

AbstractCurrently available drugs being used to treat leishmaniasis have several shortcomings, including high toxicity, drug administration that requires hospitalization, and the emergence of parasite resistance against clinically used drugs. As a result, there is a dire need for the development of new antileishmanial drugs that are safe, affordable, and efficient. In this study, two new series of synthesized quinazolinone derivatives were investigated as potential future antileishmanial agents, by assessing their activities against the Leishmania (L.) donovani and L. major species. The cytotoxicity profiles of these derivatives were assessed in vitro on Vero cells. The compounds were found to be safer and without any toxic activities against mammalian cells, compared to the reference drug, halofuginone, a clinical derivative of febrifugine. However, they had demonstrated poor antileishmanial growth inhibition efficacies. The two compounds that had been found the most active were the mono quinazolinone 2d and the bisquinazolinone 5b with growth inhibitory efficacies of 35% and 29% for the L. major and L. donovani 9515 promastigotes, respectively. These outcomes had suggested structural redesign, inter alia the inclusion of polar groups on the quinazolinone ring, to potentially generate novel quinazolinone derivatives, endowed with effective antileishmanial potential.
Hybrid convolutional bidirectional recurrent neural network based sentiment analysis on movie reviews
Authors: Soubraylu Sivakumar , Rajalakshmi Ratnavel 
Published: September 2020

AbstractSentiment analysis is the process of extracting the opinions of customers from online reviews. In general, customers express their reviews in natural language. It becomes a complex task when applying sentiment analysis on those reviews. In earlier stages, word‐level features with various feature weighting methods such as Bag of Words, TF‐IDF, and Word2Vec were applied for sentiment analysis and deep learning networks are not explored much. We considered phrase level and sentence level features instead of applying word‐level features for sentiment analysis and also enhanced by applying various deep learning techniques. In this article, we have proposed a hybrid convolutional bidirectional recurrent neural network model (CBRNN) by combining two‐layer convolutional neural network (CNN) with a bidirectional gated recurrent unit (BGRU). In the proposed CBRNN model, the CNN layer extracts the rich set of phrase‐level features and BGRU captures the chronological features through long term dependency in a multi‐layered sentence. The proposed approach was evaluated on two benchmark datasets and compared with various baselines. The experimental results show that the proposed hybrid model provides better results than any other models with an F1 score of 87.62% and 77.4% on IMDB and Polarity datasets,respectively. Our CBRNN model outperforms the state of the art by 2%‐4% on these two datasets. It is also observed that, the time taken for training is slightly higher than the existing approaches with the substantial improvement in the performance.
The effect of miRNA and autophagy on colorectal cancer
Authors: Long Jiali , He Qinglian , Yin Yuting , Lei Xue , Li Ziqi , Zhu Wei 
Published: September 2020

AbstractColorectal cancer (CRC) has become a concern because of its high recurrence rate and metastasis rate, low early diagnosis rate and poor therapeutic effect. At present, various studies have shown that autophagy is closely connected with the occurrence and progression of CRC. Autophagy is a highly cytosolic catabolic process involved in lysosomes in biological evolution. Cells degrade proteins and damaged organelles by autophagy to achieve material circulation and maintain cell homeostasis. Moreover, microRNAs are key regulators of autophagy, and their mediated regulation of transcriptional and post‐transcriptional levels plays an important role in autophagy in CRC cells. This review focuses on the recent research advances of how autophagy and related microRNAs are involved in affecting occurrence and progression of CRC and provides a new perspective for the study of CRC treatment strategies.
Hypogammaglobulinemia following heart transplantation: Prevalence, predictors, and clinical importance
Authors: Fujino Takeo , Kumai Yuto , Nitta Daisuke , Holzhauser Luise , Nguyen Ann , Lourenco Laura , Rodgers Daniel , Raikhelkar Jayant , Kim Gene , Sayer Gabriel , Uriel Nir 
Published: September 2020

AbstractHypogammaglobulinemia (HGG) can occur following solid organ transplantation. However, there are limited data describing the prevalence, risk factors, and clinical outcomes associated with HGG following heart transplantation. We retrospectively reviewed data of 132 patients who had undergone heart transplantation at our institution between April 2014 and December 2018. We classified patients into three groups based on the lowest serum IgG level post‐transplant: normal (≥700 mg/dL), mild HGG (≥450 and <700 mg/dL), and severe HGG (<450 mg/dL). We compared clinical outcomes from the date of the lowest IgG level. Mean age was 57 (47, 64) years, and 94 (71%) patients were male. Prevalence of severe HGG was the highest (27%) at 3‐6 months following heart transplantation and then decreased to 5% after 1 year. Multivariate analysis showed that older age and Caucasian race were independent risk factors for HGG. Overall survival was comparable between the groups; however, survival free of infection was 73%, 60%, and 45% at 1 year in the normal, mild HGG, and severe HGG groups, respectively (P = .013). In conclusion, there is a high prevalence of HGG in the early post‐heart transplant period that decreases over time. HGG is associated with an increased incidence of infection.
Liver transplant for inherited metabolic disease among siblings
Authors: McKiernan Patrick J. , Squires James E. , Squires Robert H. , Vockley Jerry , Mazariegos George V. , Soltys Kyle , Ganoza Armando , Strauss Kevin , Khanna Ajai , Sindhi Rakesh 
Published: September 2020

AbstractLiver transplantation is a successful option for inherited metabolic disease yet little is published on the outcome among siblings. We report outcomes of siblings who have undergone liver transplantation for metabolic disease in a single program. Seventy‐one siblings (35 males) from 33 individual families underwent liver transplantation since 1982. Outcomes were compared over three consecutive eras. Twenty‐eight families had two siblings, four had three siblings, and one had four siblings. In half of families where dates of listing were known, siblings were listed simultaneously. Mean (SD) age at listing for the oldest and second sibling was 13.2 (7.1) and 9.8 (5.7) years, respectively (p < .01). In 18/33 families, the oldest sibling underwent transplantation first. Mean (SD) age at transplant fell from the oldest to second sibling from 12.9 (7.2) to 9.5 (6.3) years, respectively (p < .001). Ten‐year patient survival was 83.5% which improved over the eras: era 1 (1982‐1994) 65.0%, era 2 (1995‐2007) 87.5%, and era 3 (2008–2019) 93.8%: p < .03. Sex, age at transplant, order of transplant, and presence of structural liver disease did not significantly impact survival. When siblings undergo liver transplant for inherited metabolic disease, later siblings are listed and transplanted at a significantly younger age.
Contrasts in alcohol‐related mortality in Czechia and Lithuania: Analysis of time trends and educational differences
Authors: Pechholdová Markéta , Jasilionis Domantas 
Published: September 2020

AbstractIntroduction and Aims.Globally, Czechia and Lithuania are among the top‐ranking countries in terms of high alcohol consumption. This study highlights notable contrasts in temporal trends in alcohol‐related mortality and identifies country‐specific patterns in educational differences.Design and Methods.The study uses harmonised cause‐of‐death series from the Human Cause of Death Database. Mortality disparities by education were assessed using census‐linked mortality data. Directly standardised death rates were used to estimate levels of national and group‐specific mortality. Relative and absolute mortality differences by education were assessed by range‐type measures (Poisson regression mortality ratios and rate differences) and Gini‐type measures.Results.Between 1994–1995 and 2016, the absolute difference between Czechia and Lithuania in terms of alcohol‐related age‐standardised death rates (per 1 000 000) decreased from 450 for males and 130 for females to 76 in males and 11 in females. In both countries, alcohol‐related mortality was markedly higher among persons of lower education levels. Lithuanian males experienced the highest absolute inequalities measured by rate difference between the low and high educated (740 per million), while Lithuanian females showed the most pronounced relative inequalities (6.70‐fold difference between low and high educated). The corresponding figures were less than half for Czechia.Discussion and Conclusions.Reducing educational disparities in alcohol‐related mortality within both countries would have a substantial impact on overall levels. Policies aimed at targeting the lowest priced and illegal alcohols and reducing levels of harmful drinking should be a priority, especially in Lithuania.
Drivers and Performance Implications of Frontline Employees’ Social Capital Development and Maintenance: The Role of Online Social Networks*
Authors: Agnihotri Raj , Mani Sudha , Chaker Nawar N. , Daugherty Patricia J. , Kothandaraman Prabakar 
Published: September 2020

ABSTRACTBuilding upon social capital theory, we identify different attributes of frontline employee (FLE) social capital and outline how the use of online social networks (OSNs) can enable social capital development and social capital maintenance. We examine key boundary conditions of time management skills, perceived innovation climate, and customer perceived FLE responsiveness. Use of multi‐informant data from FLEs working in B2B sales/service roles, their customers, and managers enabled a comprehensive analysis that accounts for the endogenous nature of the predictor variables. We find the use of OSNs relates to social capital development and maintenance. Time management skills strengthen links between OSNs and both forms of social capital. However, perceived innovation climate plays a moderating role only in the social capital development process. Unique pathways connecting social capital development to customer loyalty with the firm and social capital maintenance to FLE sales performance were noted.
Immunocytochemical characterization of primary teeth pulp stem cells from three stages of resorption in serum‐free medium
Authors: Alansary Mohammad , Drummond Bernadette , Coates Dawn 
Published: September 2020

AbstractBackground/AimsDental pulp stem cells from primary teeth cultured in serum‐free conditions may have clinical use for the repair and regeneration of teeth as well as other complex tissues and organs. The aim of this study was to test the change in the stem cell markers expression/ stem cell population in human primary pulp cells at the different stages of root resorption.MethodsCaries‐free human primary canines at defined stages of physiological root resorption were included (n = 9). In vitro cultures were established in xeno‐free, serum‐free Essential 8™ medium with human truncated vitronectin for cell attachment. An embryonic stem cell line (GENEA002) was used as a positive control. The expression of embryonic stem cell markers (Oct4, Nanog and Sox2), neural crest stem cell markers (nestin and Dlx2) and mesenchymal stem cell surface markers (CD90, CD73 and CD105) were investigated by immunocytochemistry. Mesenchymal stem cell markers CD105, CD73 and CD90 and haematopoietic markers: CD45, CD34, CD11b, CD19 and HLA‐DR were quantified with flow cytometry.ResultsThe early neural progenitor markers nestin and Dlx2 were detected in most serum‐free cultured dental pulp stem cells, regardless of the tooth resorption stage from which they were harvested. Only isolated cells were found that expressed the embryonic stem cell transcription factors Oct4A, Nanog and Sox2, and in the late stages of resorption, no Oct4A was detected. The majority expressed the mesenchymal stem cell markers CD90, CD73 and CD105. Flow cytometry found positive signals for CD90 > 97.3%, CD73 > 99.6% and CD105 > 82.5%, with no detectable differences between resorption stages.ConclusionsThis study identified populations of dental pulp cells in vitro with markers characteristically associated with embryonic stem cells, neural crest‐derived cells and mesenchymal stem cells. Flow cytometry found CD105 expressed at lower levels than CD90 and CD73. The consistency of stem cell marker expression in cells cultured from teeth at different resorption stages suggests that pre‐exfoliated primary teeth that are free of caries may provide a convenient source of multipotent stem cells for use in regenerative medicine.