bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of 31 Antimicrobials in Human Serum Using Ultra-High Performance Liquid Chromatography With Diode Array Detection for Application in Therapeutic Drug Monitoring
Authors: El-Haffaf Ibrahim , El Hassani Mehdi , Marsot Amélie 
Published: December 2024

Background:
A versatile ultra-high performance liquid chromatography method with diode array detection was developed to quantify a wide range of antibiotics in human serum. This method addresses the need for rapid and accurate determination of antibiotic levels to ensure effective patient treatment and support the fight against antibiotic resistance.

Methods:
This method assesses 31 different compounds covering β-lactams, fluoroquinolones, antifungals, antituberculars, and more. Proteins were precipitated using methanol or acetonitrile, and drugs were extracted by liquid–liquid extraction with dichloromethane. Separation of the antimicrobials was achieved on a pentafluorophenyl column, using a mobile phase of phosphoric acid (0.01 mol/L) and acetonitrile in a gradient elution mode, with a flow rate of 500 μL/min.

Results:
Almost all compounds were detected at 200 nm. The total analysis time for this method was kept under 18 minutes, including equilibration time.

Conclusions:
This efficient method enables fast determination of numerous antimicrobial classes, providing clinicians with an essential tool for ensuring effective patient treatment and combating antimicrobial resistance.
Lamotrigine Use in Lactating Women: Passage into Breast Milk and Infant Exposure: Grand Round/A Case Study
Authors: den Besten-Bertholee Daphne , Wegner Ilse , Touw Daan J. , Horst Peter G. J. , Mian Paola 
Published: December 2024

Background:
Lamotrigine, an antiseizure medication used for epilepsy and bipolar disorders, is often prescribed to women of childbearing age due to relatively low teratogenic risk. It is known that lamotrigine use in lactation leads to detectable concentrations in breast milk, although concentrations vary significantly among individuals.

Case Presentation:
A 35-year-old pregnant woman with epilepsy presented to our outpatient clinic. She was seizure-free and was taking lamotrigine 200 mg once daily. She is highly motivated to breastfeed her baby after birth but is concerned about the infant's exposure to lamotrigine through lactation. Lamotrigine concentrations were measured in both the breast milk and plasma of the suckling infants.

Conclusions:
Although lamotrigine is excreted into breast milk, and detectable in infants, the benefits of breastfeeding should not be overlooked. Higher plasma concentrations generally lead to higher breast milk concentrations, so aiming for the lowest possible maternal plasma levels is beneficial. These findings underscore the importance of TDM in this population.
Mycoplasma hominis–Associated Septic Arthritis Masquerading as Gouty Arthritis
Authors: Liddy Angelina , Kramer Carolyn , Weber Devin M. 
Published: December 2024

Abstract
Septic arthritis caused by M. hominis is exceptionally rare. Here, we report on a 62-year-old man with a medical history of gout and renal cell carcinoma who presented with persistent knee pain and swelling following partial nephrectomy complicated by an abdominal abscess. Arthrocentesis revealed monosodium urate crystals, leading to the initial diagnosis and treatment of gouty arthritis using intra-articular steroid injections, allopurinol, and colchicine. Initial cultures yielded no growth. However, faint transparent colonies grew after discharge, which were eventually identified as M. hominis. Initial antibacterial therapy targeting suspect Mycoplasma spp. was begun with doxycycline, and the patient ultimately completed 12 weeks of doxycycline and clindamycin with cure. This case underscores the importance of maintaining clinical suspicion for fastidious organisms when faced with persistent clinical symptoms without an identifiable organism and highlights a rare clinical presentation of M. hominis.
Reducing the Duration to Reach Full Enteral and Oral Feeding Volumes for Very Preterm and Extremely Preterm Infants
Authors: Mo Yu Ying , Chen Jing Jing , He Wan Xiang 
Published: December 2024

Background: Enteral and oral feeding are crucial for infants with a gestational age <32 weeks during hospitalization, with potential for improved outcomes through optimized feeding practices. Purpose: To shorten the time to achieve full enteral and oral feeding volumes in infants with a gestational age <32 weeks. Methods: This pre-post-intervention study focused on patients with a gestational age <32 weeks in Shenzhen City, 44 bed NICU. Quality improvement interventions included workflow enhancements, breastfeeding education, and oral motor strategies. Analysis of feeding times and hospital stays was conducted using SPSS software, alongside a provider survey to project feasibility. Results: Statistical significant improvements were seen in full enteral feeding time for infants under 28 weeks gestation and full oral feeding time for those between 28 and 30 weeks. Length of stay did not show significant differences between period. Providers unanimously found the intervention feasible and acceptable. Implications: Quality improvement interventions can expedite achievement of full feeding volumes in preterm infants, with potential for enhanced feeding outcomes.
Evaluating the Benefits of Endocervical Curettage in Women Infected With HPV16/18
Authors: Chen Yusha , Li Suyu , Chen Jiancui , Xue Huifeng , Zheng Xiangqin , Pan Diling 
Published: December 2024

Objective
This study evaluates the effectiveness of endocervical curettage (ECC) in detecting additional high-grade squamous intraepithelial lesions or worse (HSIL+) in women infected with human papillomavirus (HPV) types 16 and 18, which may be missed by biopsy alone.

Methods
A retrospective cohort study analyzed the medical records of 4,811 women referred for colposcopy due to HPV16/18 infection from January 2019 to December 2023. Patients underwent both biopsy and ECC. Statistical comparisons of various clinical factors between HSIL+ and low-grade or normal lesions were performed using χ2 tests and logistic regression analyses, with stratified analysis to determine ECC's additional detection rate under different clinical conditions.

Results
Endocervical curettage detected an additional 6.46% of HSIL+ lesions missed by biopsy alone, with the highest rates in women with normal colposcopic impressions (23.1%), those aged 50 or older (12%), type 3 transformation zones (9.5%), and postmenopausal women (11.9%). In contrast, younger women under 30 and those with type 1 or 2 transformation zones had significantly lower detection rates (2.9%). Logistic regression indicated that older age, type 3 transformation zone, higher grade cytological results, and abnormal colposcopic impressions are significant risk factors for HSIL+ detection via ECC (p < .001).

Conclusions
Data from this study indicate that ECC would be beneficial for women over 30 with HPV16/18 infections, particularly those with type 3 transformation zones or normal colposcopy, as it enhances HSIL+ detection. However, it offers minimal benefit for younger women or those with type 1 or 2 transformation zones.
“I Cannot Walk Far or Go Anywhere”: The Experience of Lower Limb Lymphedema Among Women With Gynecological Cancer
Authors: Hsu Yu-Yun , Liang Pei-Chi , Hsu Chia-Fang , Liu Chia-Yu , Ho Chien-Liang , Hsu Keng-Fu 
Published: December 2024

Background
Lower limb lymphedema (LLL) significantly impacts the quality of life in women who have undergone gynecological cancer surgery, limiting their physical activities, household chores, and social interactions. Despite its prevalence, there is a lack of comprehensive understanding of the lived experiences of these women.

Objective
To explore the experiences of LLL in Taiwanese women following gynecological cancer surgery.

Methods
Using a qualitative, descriptive design, 12 Taiwanese participants aged between 48 and 74 years who had LLL after gynecological cancer surgery were interviewed. Interview data were analyzed using inductive content analysis to identify key themes and subthemes.

Results
Four main themes and 13 subthemes emerged from the data: lacking awareness and vigilance, suffering from lymphedema, adapting to body image changes, and self-managing everyday life. These themes illustrate the extensive physical, psychological, and social challenges faced by the participants.

Conclusion
Findings depict the profound impact of LLL on daily life among Taiwanese women after gynecological cancer surgery. The findings highlight the need for comprehensive care that addresses the multifaceted challenges posed by lymphedema.

Implication for Practice
Oncology healthcare providers should integrate education and symptom management skills about lymphedema into patient care. In addition, there is an urgent need for accessible lymphedema care resources to support these women.
The Missing Link: Hepatitis C Linkage to Care through a Public Sexual Health Clinic
Authors: Dileepan Amirtha , Alderton Lucy , Obafemi Oluyomi , Rowan Sarah E. , Wendel Karen A. 
Published: December 2024

Abstract
This study evaluates hepatitis C testing and referral for treatment among patients attending a public sexual health clinic. In patients with hepatitis C infection, progression from linkage to care to sustained virologic response was low. Innovative approaches are needed to optimally integrate hepatitis C care in sexual health clinics.
Bioinformatics analysis of the association between miR-942-5p–induced downregulation of PIEZO-type mechanosensitive ion channel component 1 and poor prognosis in non–small cell lung cancer mediated by the mitogen-activated protein kinase pathway signaling pathway
Authors: Duan Lingdi , Zhao Min , Wei Hongquan , Dong Wei , Bi Xiaomin , Ang Lin , Zhang Shan 
Published: December 2024

Abstract

Background
Non–small cell lung cancer (NSCLC) is a common malignant tumor with an increasing incidence. PIEZO-type mechanosensitive ion channel component 1 (PIEZO1) is a mechanosensitive ion channel whose expression has been implicated in various cancers. However, its expression patterns, prognostic implications, and specific molecular mechanisms of action in NSCLC remain unclear. This study aimed to characterize the expression profile of PIEZO1 in NSCLC in vitro and using bioinformatics analyses.

Methods
To determine the expression profile of PIEZO1 in normal and cancerous human tissues and the mRNA expression of PIEZO1 in NSCLC, we analyzed data from The Cancer Genome Atlas. Various bioinformatics analyses were performed for correlation analyses, construction of survival curves, and identification of upstream mRNA targets and genes coexpressed with PIEZO1. Furthermore, the coexpressed genes were functionally annotated with Gene Ontology and subjected to pathway enrichment analyses. Additionally, we analyzed and compared PIEZO1 expression in normal and cancerous human tissue samples in vitro.

Results
NSCLC tissue samples had lower PIEZO1 expression than adjacent tissues. PIEZO1 overexpression inhibited NSCLC progression and cell migration and correlated with improved survival outcomes, as revealed by bioinformatics analyses. Additionally, analysis using the OncomiR database revealed that miR-942-5p upregulation contributed to the downregulation of PIEZO1 in NSCLC. Mechanistic investigations suggested that PIEZO1 modulates NSCLC by regulating the mitogen-activated protein kinase pathway, particularly by targeting FLNA, RRAS, and MAP3K6. Bioinformatics analyses highlighted the potential of PIEZO1 as a therapeutic target and prognostic indicator of NSCLC.

Conclusions
These novel findings reveal the expression profile of PIEZO1 in NSCLC and highlight the potential of PIEZO1 and miR-942-5p as diagnostic and therapeutic biomarkers of NSCLC and other cancer types.
Immature PIT1-lineage Pituitary Neuroendocrine Tumors/Adenomas, a Morphologically Unique Pituitary Neuroendocrine Tumors/Adenomas Commonly With Cytologic Atypia Features and a Predilection for Aggressive Clinical Potential
Authors: Lang Yuejiao , Guo Shaolei , Tuo Ying , Tian Tian , Wang Yuefeng , Li Qiming , Chen Yingqian , Chen Wenli , Zhu Yonghong , Liu Dawei 
Published: December 2024

Immature PIT1-lineage pituitary neuroendocrine tumors (PitNETs)/adenomas (Immature PIT1-lineage tumors) are a rare and underrecognized subtype of PitNETs that exhibits distinct cytologic atypia features and aggressive clinical potential. This study characterizes the clinical, radiological, histologic, and immunohistochemical features of 15 immature PIT1-lineage tumors identified from 1084 PitNETs patients over 5 years. Our cohort of 6 males and 9 females had a median age of 37.00 years (range: 23 to 68 y). All patients presented with pituitary macrotumors with an average size of 27.13×22.60×22.13 mm (length×width×height). The invasive growth pattern was identifiable, with 40.00% of tumors presenting with advanced stage (Knosp type 3 and 4) disease, followed by 20.00% Knosp type 2, 26.67% type 1, and 13.33% type 0. Clinical follow-up in 11 patients (median duration: 10.91 mo) revealed local recurrence in 1 case (9.09%). Microscopically, immature PIT1-lineage tumors comprised epithelioid (n=14) or spindle-shaped (n=1) chromophobic or weak basophilic cells with marked cytologic atypia, macronucleoli, and nuclear pseudoinclusions. By immunohistochemistry, most cases showed a consistent stain for PIT1 but limited expression of PIT1 family hormones in conjunction with diffuse or focal expression of CK8/18 (Cam 5.2), whereas none of the mimics showed a similar stain pattern in such a distinct way. We corroborate that immature PIT1-lineage tumors are rare, aggressive, and morphologically unique PitNETs/adenomas with cytologic atypia features. Immunohistochemistry may facilitate diagnosis in the distinction from histologic mimics.
Patterns of Use of β-2 Agonists, Steroids, and Mucoactive Medications to Treat Bronchiolitis in the PICU: U.S. Pediatric Health Information System 2009–2022 Database Study
Authors: Flaherty Brian F. , Olsen Cody S. , Coon Eric R. , Srivastava Rajendu , Cook Lawrence J. , Keenan Heather T. 
Published: December 2024

Objectives:
Describe β2-agonists, steroids, hypertonic saline (HTS), n-acetylcysteine (NAC), and dornase alfa (DA) use to treat bronchiolitis, factors associated with use, and associations between use and PICU length of stay (LOS).

Design:
Retrospective, multicenter cohort study.

Setting:
PICUs in the Pediatric Health Information System database.

Patients:
PICU admitted children 24 months young or younger with bronchiolitis.

Interventions:
None.

Measurements and Main Results:
We analyzed 47,520 hospitalizations between July 1, 2018, and June 30, 2022. We calculated the rate of medication use overall and the median (range) rate for each hospital: β2-agonist (24,984/47,520 [52.6%]; median hospital, 51.7% [21.4–81.7%]), steroid (15,878/47,520 [33.4%]; median hospital, 33.4% [6.0–54.8%]), HTS (7,041/47,520 [14.8%]; median hospital, 10.5% [0–66.1%]), NAC (1,571/47,520 [3.3%]; median hospital, 0.8% [0–22.0%], and DA (840/47,520 [1.8%]; median hospital, 1.4% [0–13.6%]). Logistic regression using generalized estimating equations (GEEs) identified associations between concurrent asthma and β2-agonist (adjusted odds ratio [aOR], 8.68; 95% CI, 7.08–10.65; p < 0.001) and steroid (aOR, 10.10; 95% CI, 8.84–11.53; p < 0.001) use. Mechanical ventilation was associated with all medications: β2-agonists (aOR, 1.79; 95% CI, 1.57–2.04; p < 0.001), steroids (aOR, 2.33; 95% CI, 1.69–3.21; p < 0.001), HTS (aOR, 1.82; 95% CI, 1.47–2.25; p < 0.001), NAC (aOR, 3.29; 95% CI, 2.15–5.03; p < 0.001), and DA (aOR, 7.65; 95% CI, 4.30–13.61; p < 0.001). No medication was associated with decreased PICU LOS. To assess changes in medication use over time and associations with the 2014 American Academy of Pediatrics bronchiolitis guidelines, we expanded our analysis to 83,820 hospitalizations between July 1, 2009, and June 30, 2022. Logistic regression with GEEs found no change in β2-agonist use; steroid use increased after guideline publication (aOR, 1.05; 95% CI, 1.01–1.10; p = 0.02), HTS use changed from increasing prior to the guidelines (aOR, 1.32; 95% CI, 1.11–1.56; p = 0.001) to stable since guideline publication (aOR, 0.93; 95% CI, 0.81–1.07; p = 0.33).

Conclusions:
β2-agonists, steroids, and HTS are commonly, but variably used for PICU bronchiolitis treatment. Medication use appears relatively stable over the last decade.
Postoperative Activity Restrictions After Reconstructive Pelvic Surgery
Authors: Cacheiro Bofarull Alejandra , Philip Chris Elizabeth , Francis Gabriela , Matos da Silva Pedro Henrique Costa , Koski Chloe , Murphy Linda Suk-Ling , Alzogaray Victoria , Chang Olivia H. 
Published: December 2024

Importance
Restrictive physical activity after pelvic reconstructive surgery is recommended, although the optimal duration and intensity are not standardized.

Objective
This systematic review and meta-analysis aimed to evaluate the existing literature comparing clinical outcomes for liberal postoperative physical activity versus standard of care, defined as restricted postoperative physical activity, after pelvic reconstructive surgery.

Study Design
PubMed, CENTRAL, Scopus, Web of Science, and CINAHL databases were searched for observational and randomized studies comparing liberal postoperative physical activity and standard of care in women undergoing pelvic reconstructive surgery, reporting anatomic and functional outcomes. Statistical analysis was performed using RevMan software, presenting results as mean difference (MD) or odds ratio in a random-effects model, with 95% confidence intervals (CIs).

Results
Five randomized trials, representing total n = 434, were included, with 2 studies on sling surgical procedures and 3 on prolapse surgical procedures. Data from 3 studies suggest no significant difference between liberal and standard postoperative instructions in surgical outcomes, measured by Point Ba from POP-Q assessment tool, up to 3 months follow-up (MD, −0.04; 95% CI, −0.16 to 0.07; P = 0.49). Disease-specific symptom distress, measured by Pelvic Floor Distress Inventory-20, favored the liberal approach (MD, −10.09; 95% CI, −18.33 to −1.86; P = 0.02). Other domains, including Urinary Distress Inventory-6, also showed significant improvements with liberal postoperative activities (MD, −4.29; 95% CI, −7.84 to −0.74; P = 0.02).

Conclusions
Patients with liberal postoperative physical activity recommendations in prolapse repair surgical procedures had similar short-term anatomic outcomes compared with standard restrictions, with more favorable outcomes in disease-specific symptom relief and quality of life.
95 GeV excesses in the
Z3
-symmetric next-to-minimal supersymmetric standard model
Authors: Lian Jingwei 
Published: December 2024

Recent analyses by CMS and ATLAS suggest a deviation in the diphoton channel at approximately 95 GeV, alongside a previously observed excess in bb¯ signals at a similar mass by the Large Electron-Positron collider, potentially hinting at a new scalar particle. This study explores this possibility within the framework of the Z3-symmetric next-to-minimal supersymmetric standard model. A comprehensive parameter scan was conducted, integrating constraints from dark matter relic density, direct detection experiments, and the properties of the observed 125 GeV Higgs boson. The results demonstrate that the model can accommodate the observed excesses with a singlet-dominated CP-even scalar boson near 95 GeV. The model accurately predicts signal strengths of the diphoton and bb¯ channels at a level of 1σ. Furthermore, it accounts for the measured dark matter relic abundance through bino-dominated neutralinos coannihilation with winolike electroweakinos, all while remaining consistent with existing Large Hadron Collider (LHC) constraints. These findings pave the way for future validation at the high-luminosity LHC and linear colliders, which may offer crucial tests of the model’s predictions.

Published by the American Physical Society
2024
Renormalization-group running of dimension-8 four-fermion operators in the SMEFT
Authors: Boughezal Radja , Huang Yingsheng , Petriello Frank 
Published: December 2024

We compute the renormalization-group equations governing the evolution of dimension-8 four-fermion operators in the Standard Model effective field theory (SMEFT). We describe the calculation and present analytic results for both the full flavor structure of the SMEFT and with the assumption of minimal flavor violation. We present numerical results for the renormalization-group evolution of the coefficients, and study their impact on fits of the Large Hadron Collider (LHC) Drell-Yan data. The effects of running on the dimension-8 coefficients can reach 50% or more when evolving from 10 TeV scale down to few-GeV energies relevant for the analysis of fixed-target data. However, the impact of the dimension-8 running on the analysis of Drell-Yan data from the LHC is minimal.

Published by the American Physical Society
2024
Images of ingredients or finished products on food packages: the effects of food readiness and purchasing motivations
Authors: Huang Li-Shia , Chou Yu-Jen , Zhang Cheng , Lan Ying-Yu 
Published: December 2024

PurposeThis study explores the impact of packaging images (ingredients vs finished products), degree of food readiness (ready-to-heat vs ready-to-cook) and purchasing motivations (utilitarian vs hedonic) on consumer preferences.Design/methodology/approachTwo 2 × 2 between-subject experiments were conducted to investigate the interactive effects of package image type and food readiness (Study 1) as well as their individual impacts (Study 2). Data analysis and hypothesis testing were performed using SPSS software.FindingsConsumers initially preferred packaging images of finished products over ingredients when selecting ready-to-eat foods. However, their attitudes declined upon learning about specific food content. When motivated by hedonic factors, consumers favored images of finished products for ready-to-cook foods, whereas utilitarian motivations led to a preference for ingredient images. After purchasing, ingredient images were favored across both motivational contexts.Originality/valueFood packaging images serve as crucial cues in consumer purchase decisions, though no consensus currently exists on whether these images should feature ingredients or finished products. This study offers valuable insights into how these visual factors affect consumer behavior and decision-making, providing manufacturers with guidance on enhancing product evaluations by consumers.