bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vancomycin-Lock Therapy for Prevention of Catheter-Related Bloodstream Infection in Very Low Body Weight Infants
Authors: Liang Hong , Zhang Lian , Guo Xiaoping , Sun Li 
Published: June 2020

Abstract
Background: This study was to evaluate the effectiveness and safety of vancomycin- lock therapy for the prevention of catheter-related bloodstream infection (CRBSI) in very low body weight (VLBW) preterm infant patients.Methods: One hundred and thirty-seven cases of VLBW preterm infants who retained peripherally inserted central catheters (PICCs) were retrospectively reviewed, including 68 treating with heparin plus vancomycin (vancomycin-lock group) and 69 with heparin only (control group). The incidence of CRBSI, related pathogenic bacteria, adverse events during the treatment, complications, antibiotic exposure, PICC usage time, hospital stay, etc. were compared between the above two groups.Results: The incidence rate of CRBSI in the vancomycin-lock group (4.4%, 3/68) was significantly less than in the control group (21.7%, 15/69, p=0.004). Total antibiotic exposure time during the whole observation period was significantly shorter in the group than in the control group (11.2±10.0 vs 23.6±16.1 d; p<0.001). No hypoglycemia occurred during the locking, and the blood concentrations of vancomycin were not detectable.Conclusions: Vancomycin-lock may effectively prevent CRBSI in Chinese VLBW preterm infants, prolong the usage time of PICC line, and reduce the exposure time of antibiotics, without causing obvious side complications.
Molecular Detection of Rickettsia in Fleas from Micromammals in Chile with Potential Public Health Implications
Authors: Salas Lucila Moreno , Carniglia Mario Espinoza , Schmeisser Nicol Lizama , Fuentes Luis Gonzalo Torres , Fuente María Carolina Silva de la , Lareschi Marcela , Acuña Daniel González 
Published: June 2020

Abstract
BackgroundFleas are important vectors of pathogenic bacteria that pose public health concerns worldwide, including Rickettsia. Micromammals, and especially rodents, are the main flea hosts, therefore they play a fundamental role in the spread of flea-borne diseases and various species of rodent fleas can also parasitize humans. In addition to this, many rodent species are capable of inhabiting wild environments and adapting to rural and urban environments, which could favor a continuous gradient of transmission between domestic and wild species. The aim of this study was to detect, characterize, and compare Rickettsia spp. from the fleas of micromammals in areas with different human population densities in Chile.MethodsTo determine Rickettsia in fleas of micromammals, we analyzed 1,315 fleas obtained from 1,512 micromammals, which were captured in 29 locations with different human population densities in Chile. We used polymerase chain reaction (PCR) amplification of the gltA, rpoB, and ompB genes to detect Rickettsia in fleas.ResultsThe prevalence of Rickettsia among flea species ranged from 0%–35.1%. Fifteen flea species were found to be Rickettsia-positive. Areas of lower human density have the highest prevalence of Rickettsia. The phylogenetic tree shows two well-differentiated clades. Rickettsia belli is positioned as basal in a clade. Another clade is subdivided into two subclades, and are related to Rickettsia of typhus group.ConclusionsConsidering that fleas are generalist parasites, and that they are frequently found and abundant in rodents, which are closely related to humans, the detection of potential emerging zoonotic pathogens in rodent fleas highlights the risk of infection to humans.
Nondestructive estimation of potato yield using relative variables derived from multi-period LAI and hyperspectral data based on weighted growth stage
Authors: Luo Shanjun , He Yingbin , Li Qian , Jiao Weihua , Zhu Yaqiu , Zhao Xihai 
Published: June 2020

Abstract
Background
The accurate estimation of potato yield at regional scale is crucial for food security, precision agriculture and agricultural sustainable development.
Methods
In this study, we developed a new method using multi-period relative vegetation indices (rVIs) and relative LAI (rLAI) data to improve the accuracy of potato yield estimation based on weighted growth stage. Two experiments of field and greenhouse (water and nitrogen fertilizer experiments) in 2018 were performed to obtain the spectra and LAI data of the whole growth stage of potato. Then the weighted growth stage was determined by three weighting methods (improved analytic hierarchy process method, IAHP; entropy weight method, EW; and optimal combination weighting method, OCW) and Slogistic model. A comparison of the estimation performance of rVI-based and rLAI-based models with single stage and weighted stage was completed.
Results
The results showed that among the six test rVIs (rNDVI, rCIred edge, rCIgreen, rEVI2, rNDRE and rMTCI), rCIred edge was the optimal index of the single-stage estimation models with the correlation with potato yield. The most suitable single stage for potato yield estimation was the tuber expansion stage. For weighted growth stage models, the OCW-LAI model was determined as the best one to accurately predict the potato yield with adjusted R2 value of 0.8333, and the estimation error about 8%.
Conclusion
This study emphasizes the importance of inconsistent contributions of multi-period or different types of data to the results when they are used together, and the weights need to be considered.
Mel Aggravates Pyroptosis Induced by Temozolomide Through Inhibiting NRF2-are Pathway in Glioblastoma
Authors: Pan Hao , wang Handong , Wang Qiang , Niu Wenhao , Wu Qi , Jia Yue , Sun Kangjian , Chen Longbang 
Published: June 2020

Abstract
Background:Glioblastoma(GBM) is a common malignant tumor of the brain. It has been verified that melatonin(MEL) can inhibit glioma proliferation. But its mechanism has not been fully elucidated. We intend to examine the mechanism of MEL on glioma cells from the perspective of pyroptosis and Nrf2. Methods:Expression of MEL receptor in glioma was detected by western blot. GBM cell viability treated with temozolomide(TMZ) plus MEL was detected by CCK-8. Pyroptosis rate was determinate by flow cytometric analysis. Western blot was used to detect the Nrf2 and pyroptosis related protein level after MEL treatment. Orthotopic tumor growth inhibition study was performed to further investigate the tumor inhibition effect of TMZ plus MEL.Results:We first confirmed MEL receptor was abundant in glioma tissue and cell lines. After combined treatment of TMZ and MEL, cell viability decreased significantly as compared to those of TMZ treatment alone. Also, the ratio of pyroptosis and ROS level increased, followed by elevated expression of pyroptosis related protein. Furthermore, MEL can induce a diminution of Nrf2 expression in glioma in dose- and time-dependent manner. TMZ can increase Nrf2-ARE pathway expression, which also can be deprived by MEL. Its inhibition of Nrf2 depends on dephosphorylation of IGF-1/AKT/mTOR pathway. More importantly, after overexpression of Nrf2 in glioma cells, the level of pyroptosis-related protein elevated by MEL decreased, suggesting that the effect of MEL on promoting pyroptosis is dependent on its inhibition of Nrf2. In vivo results further confirmed that MEL plus TMZ induced significantly decreased tumor size and increased pyroptosis rate, but had no significant effect on mouse body weight, ALT, AST.Conclusion:MEL can inhibit the phosphorylation of IGF-1/AKT/mTOR pathway at millimol level, which further reduces the expression of Nrf2 and promotes pyroptosis of glioma cells. Considering the modest efficacy of TMZ chemotherapy, MEL can be considered as a potential chemotherapy sensitizer to improve the chemotherapy effect of glioma.
PRDM16 inhibits cell proliferation and migration via epithelial to mesenchymal transition by directly targeting pyruvate carboxylase in papillary thyroid cancer
Authors: Liu Wan-Lin , Guan Qing , Wen Duo , Ma Ben , Xu Wei-Bo , Hu Jia-Qian , Wei Wen-Jun , Li Duan-Shu , Wang Yu , Xiang Jun , Liao Tian , Ji Qinghai 
Published: June 2020

Abstract
Background
PRDM16 (known as MEL1), a member of PR domain zinc finger family, has been implicated in multiple biological processes including cancers. It is not clearly yet whether PRDM16 is involved in tumor progress of papillary thyroid cancer (PTC).
Methods
We identified PRDM16 expression level in PTC tissues by qRT-PCR and analyzed its relationship with clinical characteristics in both Fudan University Shanghai Cancer Center (FUSCC) cohort and TCGA cohort. We tested the function of PRDM16 in PTC cells both in vivo and in vitro. We found direct downstream target of PRDM16, pyruvate carboxylase (PC) by RNA-sequencing, rescue experiments, Luciferase assay and Chromatin Immunoprecipitation assay.
Results
PRDM16 was downregulated in papillary thyroid cancer tissues and was significantly related with lymph node metastases and extrathyroid extension in both FUSCC and TCGA cohorts. Overexpression of PRDM16 could attenuate proliferation and migration of PTC cells via inhibiting epithelial to mesenchymal transition process. PC was upregulated in papillary thyroid cancer tissues. Knockdown of PC could inhibit proliferation and migration in TPC-1 and K1 cells. The repression effect on cell proliferation and migration from PRDM16 was PC dependent. PRDM16 could directly bind to PC promoter and inhibited its expression at transcription level. Moreover, the mRNA expression level of PRDM16 and PC was negatively related in human PTC tissues.
Conclusions
In conclusion, PRDM16 exhibited anti-tumor effect and EMT inhibition function in PTC by directly binding with PC promoter. PRDM16 may be a novel therapeutic target in papillary thyroid cancer.
Disentangling Microbial Syntrophic Mechanisms for Hexavalent Chromium Reduction in Autotrophic Biosystems
Authors: Zhang Baogang , Liu Jun , Sheng Yizhi , Shi Jiaxin , Dong Hailiang 
Published: June 2020

Abstract
Background: Hexavalent chromium [Cr(VI)] is one of ubiquitous heavy-metal contaminants in groundwater, and electron donors are considered to be a key parameter for Cr(VI) biotransformation. During autotrophic remediation process, however, much remains to be unveiled that how complex syntrophic microbial communities couple Cr(VI) reduction with other elemental cycles. Results: Two series of Cr(VI)-reducing groundwater bioreactors were independently amended by elemental sulfur (S0) and iron (Fe0), and inoculated with the same inoculum. After 160 days incubation, both bioreactors showed the similar archaea-dominating microbiota compositions, whereas a higher Cr(VI) reducing rate and more methane production were detected in the Fe0-driven one. Metabolic reconstruction of 23 retrieved genomes revealed complex symbiotic relationships driving distinct elemental cycles coupled with Cr(VI) reduction in bioreactors. In both bioreactors, these inferred Cr(VI) reducers were assumed to live in syntrophy with oxidizers of sulfur, iron, and volatile fatty acids (VFAs) and methane produced by carbon fixers and multi-trophic methanogens, while hydrogen slowly released via an Fe0 corrosion process might readily facilitate methanogenesis and more methane oxidation might be linked to Cr(VI) reduction in the Fe-bioreactor.Conclusion: These findings provide insights into mutualistic symbioses of carbon, sulfur, iron and chromium metabolisms in groundwater systems, providing implications for both in-situ and ex-situ bioremediation of contaminated groundwater.
Construction and Analysis of lncRNA-mediated ceRNA Network in Nasopharyngeal Carcinoma Based on Weighted Correlation Network Analysis
Authors: Zou Zhenning , Liu Shuguang , Ha Yanping , Huang Bowan 
Published: June 2020

Abstract
Background: Increasing evidence indicated that aberrant expression of long noncoding RNAs (lncRNAs) are involved in tumorigenesis and progression of nasopharyngeal carcinoma (NPC). The purpose of this study is to construct a lncRNA-mediated ceRNA network based on Weighted correlation network analysis (WGCNA) and explore the relationship between the constituents of the ceRNA network and the prognosis of patients with NPC.Methods: In this study, WGCNA was performed on the GSE102349 to find modules of highly correlated genes. The preservation of the modules was examined by GSE68799. GSE12452 was utilized to identify the differentially expressed lncRNAs and mRNAs. GSE32960 was utilized to identify the differentially expressed miRNAs. Through annotation and intersection, the lncRNAs and mRNAs in the same WGCNA modules and the miRNAs were used to construct a ceRNA network. Next, the prognostic value of the constituents of the ceRNA network was evaluated by survival analysis. Furthermore, a risk score model for predicting progression-free survival (PFS) of NPC patients was established via lasso penalized Cox regression based on the differentially expressed genes, and the differences in the expression of the lncRNAs and mRNAs in the ceRNA network between high- and low-risk groups were investigated.Results: Fourteen stable modules were identified using GSE102349 based on WGCNA. A ceRNA network composed of 11 lncRNAs, 15 miRNAs and 40 mRNAs was established. The lncRNAs and mRNAs in this network were from turquoise and salmon modules. Survival analysis indicated that ZNF667-AS1, four mRNAs (LDHA, LMNB2, TPI1, and UNG), and hsa-miR-142-3p in the ceRNA network were significantly correlated with the prognosis of NPC. Gene set enrichment analysis indicated that the up-regulation of ZNF667-AS1 was associated with some immune-related pathways. Besides, a risk score model consisting of 12 genes was constructed and showed a good performance in predicting PFS in NPC patients. Among the 11 lncRNAs in the ceRNA network, SNHG16, SNHG17, and THAP9-AS1 were up-regulated in the high-risk group of NPC, while ZNF667-AS1 was down-regulated in the high-risk group of NPC.Conclusions: These results will promote our understanding of the crosstalk among lncRNAs, miRNAs, and mRNAs in the tumorigenesis and progression of NPC.
HOXA-AS2 Promotes Glioblastoma Carcinogenesis by Targeting miR-885-5p Thus Releasing RBBP4 mRNA
Authors: Shou Jixin , Gao Haidong , Cheng Sen , Wang Bingbing , Guan Haibo 
Published: June 2020

Abstract
Background: HOXA-AS2, a lncRNA, had been proved to exert the promoting effect on glioblastoma, but its regulatory mechanism was not fully revealed. Our study focused on the interaction and role of HOXA-AS2/miR-885-5p/RBBP4 axis in the development of glioblastoma. Methods: qRT-PCR was used to detect the expression of lncRNA, miRNA and mRNA in glioblastoma tissues and cells. Dual luciferase assay, RIP assay and RNA pull-down assay were applied to reveal the interaction among HOXA-AS2, miR-885-5p and RBBP4. The cell functional experiments such as CCK-8, BrdU assay and cell adhesion etc. were used to analyze the effect of HOXA-AS2/miR-885-5p/RBBP4 axis on glioblastoma cells. Results: HOXA-AS2 and RBBP4 were overexpressed in 33 glioblastoma tissue samples and glioblastoma cells, as well as HOXA-AS2 and RBBP4 contributed to the tumorigenesis of glioblastoma cells. On the other hand, miR-885-5p was proved to be down-regulated in 33 glioblastoma tissue samples and glioblastoma cells, and it was an obstacle to the malignant phenotypes of glioblastoma cells. Meanwhile, HOXA-AS2 could negative regulate miR-885-5p, thereby promoting the RBBP4 expression since RBBP4 was targeted by miR-885-5p. Finally, HOXA-AS2 was proved to act as the positive role in glioblastoma cells via negative regulating miR-885-5p to release RBBP4.Conclusion: HOXA-AS2 promotes the tumorigenesis of glioblastoma through targeting miR-885-5p to induce the RBBP4 expression.
Effect of low dose Bupivacaine with Fentanyl on hemodynamic response: A Prospective cohort study
Authors: Obsa Mohammed Suleiman , Negash Behaku Awel , Kassa Adugna Aregawi , Muzeyan Suleiman Jemal , Helill Shamill Eanga 
Published: June 2020

Abstract
Background: A conventional dose of local anesthetics is used during spinal anesthesia. However an unwanted hemodynamic effect by administration of adjuvants with low dose of local anesthetics has been observed. The purpose of the study was to compare the effect of low dose Bupivacaine with Fentanyl versus normal dose Bupivacaine alone on hemodynamic response in elderly patients. Methods: A Prospective cohort study was employed on a total of 64 elderly patients undergoing lower extremity orthopedics surgery. An exposed group (group BF) received 10mg of 0.5% isobaric bupivacaine with 25mcg of fentanyl and a non-exposed group (Group B) received 15 mg of 0.5% isobaric bupivacaine alone. Systematic random sampling technique was used to select study participants. Parametric data was analyzed by using independent t-test, nonparametric data by using Mann-Whitney U-test and homogenous categorical data by using chi-square test and fisher exact test. The level of statistical significance for all tests was found to be P < 0.05. Results: The incidence of hypotension was higher in group B than group BF (37.5% vs. 9.4%, respectively) and statistically significant differences was observed (p=0.008). The onset of sensory block and regression of motor block to zero were faster in group BF than in group B (p <0.05). TAR was also prolonged in group BF (P<0.05). Conclusion: Low dose Bupivacaine with Fentanyl has lower incidence of hypotension, fast onset of sensory block and prolonged first analgesia request time than bupivacaine alone Therefore, we recommends the use of low dose bupivacaine with fentanyl to have a better hemodynamic stability than conventional dose of bupivacaine for elderly patients who undergoes lower extremity surgery.
Proline-rich Protein 11 Overexpression is Associated With a More Aggressive Phenotype and Poor Overall Survival in Ovarian Cancer Patients
Authors: zhan yu , wu xueyuan , zheng gang , jin jingjing , li chaofu , yu guanzhen , li wenfeng 
Published: June 2020

Abstract
Background: The proline-rich protein 11 (PRR11) is a newly identified oncogene that exerts a strong association with a poor prognosis in various kinds of human cancers, yet its role in ovarian cancer (OC) remains unknown. Thus, the study aims to evaluate the level of PRR11 protein expression and its function in human ovarian cancer.Methods: In the present study, we used immunohistochemistry analysis to evaluate the levels of PRR11 protein expression in human specimens from 49 OC patients who underwent curative surgery in the First Affiliated Hospital of Wenzhou Medical University2007 to 2015.Results: In our analysis, 57.1% of the primary OC tumor tissue evaluated, presented over expression of PRR11. Meanwhile, survival analysis showed that the overall survival (OS) of patients presenting PRR11 over expression was significantly lower than the OS of the PRR11 negative patient group. Furthermore, in subsequent experiments we found that silencing PRR11 expression inhibited tumor cell proliferation and cell migration in vitro. In addition, cells subjected to PRR11 knockdown exhibited a decrease in tumor grow in vivo. The down regulation of PRR11 was acompanied by a decrease in N-cadherin and early growth response protein 1 (EGR1) expression.Conclusions: our results suggest that PRR11 may be considered as a potential target for prognosis assessment and gene therapy modalities for patients with OC.
Pulmonary toxicity and inflammatory response of e-cigarettes containing medium-chain triglyceride oil and vitamin E acetate: Implications in the pathogenesis of EVALI but independent of SARS-COV-2 COVID-19 related proteins
Authors: Muthumalage Thivanka , Lucas Joseph H. , Wang Qixin , Lamb Thomas , McGraw Matthew D. , Rahman Irfan 
Published: June 2020

Abstract
Recently, there has been an outbreak associated with the use of e-cigarette or vaping products, associated lung injury (EVALI). The primary components of vaping products, vitamin E acetate (VEA) and medium-chain triglycerides (MCT) may be responsible for acute lung toxicity. Currently, little information is available on the physiological and biological effects of exposure to these products. We hypothesized that these e-cig cartridges and their constituents (VEA and MCT) induce pulmonary toxicity, mediated by oxidative damage and inflammatory responses, leading to acute lung injury. We studied the potential mechanisms of cartridge aerosol induced inflammatory response by evaluating the generation of reactive oxygen species by MCT, VEA, and cartridges, and their effects on the inflammatory state of pulmonary epithelium and immune cells both in vitro and in vivo. Cells exposed to these aerosols generated reactive oxygen species, caused cytotoxicity, induced epithelial barrier dysfunction, and elicited an inflammatory response. Using a murine model, the parameters of acute toxicity to aerosol inhalation were assessed. Infiltration of neutrophils and lymphocytes was accompanied by significant increases in IL-6, eotaxin, and G-CSF in the bronchoalveolar lavage fluid (BALF). In mouse plasma, eicosanoid inflammatory mediators, leukotrienes, were significantly increased. Plasma from e-cig users also showed increased levels of hydroxyeicosatetraenoic acid (HETEs) and various eicosanoids. Exposure to e-cig cartridge aerosols showed the most significant effects and toxicity compared to MCT and VEA. In addition, we determined at SARS-COV-2 related proteins and found no impact associated with aerosol exposures from these tested cartridges. Overall, this study demonstrates acute exposure to specific e-cig cartridges induces in vitro cytotoxicity, barrier dysfunction, and inflammation and in vivo mouse exposure induces acute inflammation with elevated pro-inflammatory markers in the pathogenesis of EVALI.