bluuebunny/crossref_metadata_2025_split huggingface dataset:
The hypercoagulation state among major Î²-thalassemia patients at H. Adam Malik Hospital, Medan, Indonesia
Authors: Silitonga Jane Tetraulina , Aman Adi Koesoema , Lubis Bidasari 
Published: April 2020

Background:Â Thalassemia is the most common hereditary blood disorder in the world and Indonesia.Â MajorÂ Î²-thalassemiaÂ is classified asÂ Transfusion-Dependent Thalassemia (TDT).Â Specific changes in the composition of red cell membrane lipids and hemosiderosis may lead to hypercoagulation. This study aimed to determine the difference in Protein C, Protein S, Antithrombin III (AT-III), and D-dimer between majorÂ Î²-thalassemiaÂ patients and normal people.Methods:Â This was an observational analytical study with a cross-sectional design, including 18 major Î²-thalassemia patients in H. Adam Malik Hospital Medan who met the study criteria and 18 normal controls. Subjects were examined for Protein C, Protein S, AT-III and D-dimer. The assessment was also carried out among patients based on their transfusion frequency and serum ferritin.Results: There were significant differences in Protein C, Protein S, and D-dimer levels between majorÂ Î²-thalassemiaÂ patients and normal controls (p < 0.05). AT-III level in majorÂ Î²-thalassemiaÂ patients was lower than healthy controls, however the difference was not significant. The level of Protein C in patients who infrequently and frequently underwent blood transfusions were 34.88 Â± 8.6 and 45.19 Â± 7.8 respectively, which was significantly different (p < 0.05). A significant differences in Protein C, Protein S and D-dimer were also found between patients with serum ferritin level < 2,500 ng/ml and Â â‰¥ 2500 ng/ml.Conclusion:Â Significant changes were found in anticoagulation proteins of major Î²-thalassemia patients. Frequent blood transfusions and chelation therapy should be emphasized for major thalassemia patients because they affect anticoagulation protein levels.
The comparison of erythrocyte sedimentation rate (ESR) modify Westergren Caretium Xc-A30 and Westergren Manual in Clinical Pathology Laboratory, Sanglah General Hospital, Denpasar, Bali
Authors: Witarini Dewi Made Minarti , Herawati Sianny , Mulyantari Ni Kadek , Prabawa I Putu Yuda 
Published: April 2020

Â Background: The erythrocyte sedimentation rate (ESR) test is one of the most widely performed laboratory test to assess acute phase of inflammation, infection, autoimmune or malignancy. The reference method for ESR measurement was introduced by Westergren. This method is generally easy, un-expensive, yet, time consuming. Automatic instrument has been introduced to decrease the measurement time. Caretium XC-A30 is automatic instrument to measure ESR; it is used in Sanglah General Hospital Denpasar without previous research. Aim of this study was to assess comparison, correlation and agreement of Caretium XC- A30 to the reference method.Methods: A total of 35 samples in consecutive sampling were taken during August 2018 at Sanglah General Hospital, Denpasar. The samples were examined for ESR with Caretium XC-A30 and manual Westergren. Comparison, correlation and agreement were tested for both methods used.Results: The Westergren method was the reference method and Caretium XC-A30 was the tested method. Wilcoxon signed rank test showed no difference between two methods (p= 0.439). There was a very strong correlation and excellent agreement showed by Spearman coefficient correlation r= 0.989 and kappa coefficient 0.942. Conclusion: This study showed no difference between Caretium XC-A30 and Westergren as the reference method for ESR measurement, with very strong correlation and excellent agreement. Thus, Caretium XC-A30 has a potential use in performing ESR measurement with high throughput and lesser time than manual Westergren method.
The incidence of Clostridium Difficile infection in diarrhea patients after receiving antibiotics at Dr. Soetomo Hospital Surabaya
Authors: Chrisnanda Rima Hayyu , Wardhani Puspa 
Published: April 2020

Introduction: Improper use of antibiotics is a risk factor for C. difficile infection. The increasing incidence of C. difficile infection is one of the indications of failure in infection prevention and control in hospitals. The aim of this study was to analyze the incidence of C. difficile infection in diarrhea patients after receiving antibiotics in the ICU and internal medicine ward of Dr. Soetomo Hospital Surabaya.Methods: Stool samples were taken from 31 diarrhea patients with at least 2 x 24-hours of antibiotic use who were admitted to the ICU and hospitalized at Dr. Soetomo Hospital Surabaya from August 2017 to May 2018. Each sample was examined for glutamate dehydrogenase (GDH) and A and B toxins of C. difficile. The results of this study were analyzed descriptively.Results: The average age of respondents was 48.94 (21 â€“ 86) years old. In addition, based on demographic data, it was predominant by females (65%), diabetes mellitus and fever (16%), and ceftriazone usage (22%). Â The results showed one sample (3%) with positive results and 30 samples (97%) with negative results for both A/B toxins and GDH enzyme.Conclusion: It can be concluded that the incidence of diarrhea in these patients was not due to C. difficile infection. The cause of diarrhea in these patients could be due to other causes such as a virus, fungus, or other bacterial infections. More sample collection is needed to provide more accurate data about C. difficile infection, so it can help to fulfill data for infection prevention and control in hospitals.
The ability of earthworm Lumbricus rubellus extract in slowing down the activation of NFkB and TNF-Î± in lipopolysaccharide-induced Rattus norvegicus
Authors: Foekh Neiny Prisy , Sukrama I Dewa Made , Lestari Anak Agung Wiradewi 
Published: April 2020

Background: Earthworm (L. rubellus) contains many compounds such as Lumbricin I, G-90 glycoprotein, and polyphenols as antimicrobial, antioxidant, and hepatoprotective toward bacterial infections. Tumor necrosis factor (TNF-Î±) has been identified as a mediator for necrosis tumor inside animal serum treated by lipopolysaccharide (LPS). High concentrated LPS can cause tissue damage and death. The increasing number of Nuclear Factor Kappa Beta (NFkB) activation has been responded by macrophage to produce cytokines pro-inflammation TNF-Î± as an indicator of inflammation.Methods: The research was conducted in the Animal Laboratory Pharmacology Unit, Biomolecular Laboratorium, Faculty of Medicine Udayana University and the Pathology Clinic Sanglah General Hospital with a post-test only control group design. Thirty-two experimental rats were made as samples and divided into four groups, which are negative control, positive control, treatment 1, and treatment 2. Treatment was performed for 14 days. The containment of NFkB and TNF-Î± was measured on the 16thÂ day using quantitative technique sandwich enzyme immunoassay (ELISA).Results: The results of the Shapiro-Wilk test and Leveneâ€™s test showed that the data were normally distributed and homogeneous, with p > 0.05. As the data were normally distributed, the one way ANOVA was used to analyze the data, followed by the Least Significant Difference (LSD) test. There was a significant difference between the average amount of TNFÎ± found in each treatment group p = 0.001 (< 0.05). There were also significant differences between the average amount of NFkB found in each treatment group p = 0.001 (< 0.05). Advanced test using LSD shows more significant differences by p < 0.05. Conclusion: It can be concluded that earthworm extract (L.rubellus) raises the amount of NFkB and TNF-Î± in male experimental rats injected with LPS.
A rare case of Hb H disease caused by compound heterozygous for Î± thalasemia and Hb Quong Sze in Chinese Indonesian proband: a case report
Authors: Widyastiti Nyoman Suci , Nainggolan Ita Margaretha , Kurnia Edward L , Retnoningrum Dwi , Budiwiyono Imam 
Published: April 2020

Background: Hemoglobin H (HbH) disease is alpha thalassemia characterized by inactivation of three of four Î±-globin genes due to deletions with or without non-deletional Î±-thalassemia. Hb Quong Sze (Hb QS) is a very rare non-deletional Î±-thalassemia in Indonesia caused by a CTGLeu>CCGPronucleotide substitution at codon 125 of Î±2 globin gene generating highly unstable hemoglobin. Compound heterozygosity for Hb QS and Southeast Asian double Î±-globin gene deletion (--SEA) result in accumulation of b-globin tetramers, causing hemolytic anemia.Case Report: A 49 years old Chinese Indonesian female was assessed for thalassemia screening. The phenotype of the proband was normal and only mild anemia was noticeable. She experienced blood transfusion five years ago due to a sudden fall of hemoglobin level after malarial infection. Complete blood count found hemoglobin 8.3 g/dL, Mean Corpuscular Volume (MCV) 65.7 fl and Mean Corpuscular Hemoglobin (MCH) 17.1 pg. HbH disease suggested by abundant Hb H inclusion bodies in the red blood cells. Microcapillary hemoglobin electrophoresis result showed HbH 31.8 %, Hb Bart 0.4%, HbA 67.3% and HbA2 0.5%. Molecular studies were carried out using multiplex polymerase chain reaction (PCR) method, and the common a0-thalassemia(--SEA) was detected in one allele. Direct sequencing analysis of the Î±1 and Î±2 globin genes revealed Hb QS in the other allele.Conclusion: Non-deletional Hb H disease due to compound heterozygous ofÂ Hb QS with Southeast Asian double Î±-globin gene deletion (--SEA) has a very low incidence in Indonesia. An advanced molecular analysis should be performed to determine this rare mutation.
Geriatric fractures in single Orthopedic Hospital: The role of domestic fall and comprehensive geriatric assessment
Authors: Irianto Komang Agung , Rianto Dionisius , Sukmajaya William Putera , Alina Oen 
Published: April 2020

Background: The geriatric population has continued to grow globally. The incidence of geriatric fractures increases along with the population. The geriatric assessment could be useful to build a warning sign for the fall risk.Aim: The authors aimed to describe the geriatric fractures; the factors affecting; to further give recommendation regarding its prevention and management.Methods: This is a cross-sectional study involving 741 patients with geriatric fractures admitted to Surabaya Orthopedics and Traumatology Hospital from 2012-2017. All data acquired from medical records.Results: The number of female patients in our study are three times the male patients. The distribution of the cases across ethnicity, gender, and different age group were quite similar. The case of vertebral and hip fracture was significantly higher. Domestic accident was the mechanism of injury in 80% of all cases, and this MOI is significant across all fracture types. Only 32% patients do not have confounding disease. However 25% has diabetes mellitus, and 42% has hypertension.Â Although patients with diabetes mellitus, hypertension, moderate to severe anemia, and hyponatremia were more frequent but not significant to be the risk factor for certain fracture type.Conclusion: Domestic accident, female, and cofounding disease were the most common factors found for all type of fractures. The most common geriatric fractures admitted to our institution were hip and vertebral fractures.
Analysis of nerve growth factor serum levels in psoriasis vulgaris patients
Authors: Novatina E. Pangaribuan Riana , B. Putra Imam , A. Nababan Kristo 
Published: April 2020

Background: Psoriasis is a complex chronic inflammatory skin disease with multifactorial mechanism affected by immunological, genetic, environmental and nervous system factors which its mechanism is still unknown. The involvement of nervous system in psoriasis is ruled by nerve growth factor (NGF). Increased levels of NGF serum are expected occurring in psoriasis vulgaris.Aim: To determine the differences between NGF serum levels in psoriasis vulgaris patients and controlsMethods: This study is an analytic study with cross sectional design involving 30 psoriasis vulgaris patients and 30 healthy controls. Diagnosis of psoriasis vulgaris was made based on history and clinical examination; we conducted blood sampling and measurement of NGF serum level to the patients and controls. The differences between NGF serum levels in psoriasis vulgaris patients and controlled is analyzed by independent-t test. This research approved by the health research ethics committee of the Faculty of Medicine of Sumatra Utara University/ H. Adam MalikÂ Medan General Hospital.Results: In this study, we found the mean NGF serum level of female psoriasis vulgaris patients (810.30 Â± 299.97 pg/ml)Â was higher compared to male (806.70 Â± 256.04 pg/ml).Â The mean NGF serum of psoriasis vulgaris patients were highest on ages group 15 â€“ 23 years (930.14 Â± 69.03 pg/ml). The mean NGF serum level of psoriasis vulgaris without family history (875.38 Â± 203.91 pg/ml) was higher compared to patient with a maternal history (600.87 Â± 387.45 pg/ml) and paternal history (477.88 Â± 406.97 pg/ml). The mean NGF serum level in the psoriasis vulgaris group (808.38 Â± 272.48 pg/ml) was higher compared to the control group (524.92 Â± 281.22 pg/ml) dengan with p=0.0001.Conclusion: There were significant differences in NGF serum levels between psoriasis vulgaris patients and control.
The effect of Sargassum duplicatum extract on SGPT level in Rattus norvegicus infected by Plasmodium berghei
Authors: Setianingsih Herin , Pamungkas Dimas Bathoro Bagus , Utami Prawesty Diah 
Published: April 2020

Background: Malaria still becomes a major health problem in Indonesia, especially in the eastern part of Indonesia. The occurrence of Plasmodium resistance against antimalarial medications increases the difficulty of malaria wipe-out effort. As regard with such a great potential Sargassum production that Indonesia possesses, it is very likely to develop Sargassum as adjuvant therapy to prevent and even to stop the resistance.Aim: This research aims to develop adjuvant therapy of Sargassum extract to avoid the resistance of malaria therapy on the SGPT level.Method: There were 24 white rats (Rattus norvegicus) that had been infected by Plasmodium berghei and were given dihydroartemisinin-piperaquin (DHA-PPQ) therapy as antimalarial therapy at the dose of 3mg/KgBW for three consecutive days. The rats were later divided into three groups. Group 1 received only (DHA-PPQ), group 2 received Sargassum duplicatum extract as adjuvant therapy at Â the dose of 100 mg/KgBW for ten consecutive days. Group 3 also received Sargassum duplicatum extract as adjuvant therapy but the dose was at 300 mg/KgBW for ten consecutive days.Result: SGPT level decreased more significantly in groups in which the rats received dihydroartemisinin-piperaquine and 100 mg/kgBW Sargassum extract as adjuvant therapy and on the other hand only received Sargassum extract at the dose of 300 mg/kgBW. After ten days of therapy the blood SGPT level was examined from all rats. The data were analyzed using one-way ANOVA test.Conclusion: Sargassum duplicatum (100 mg/kgBW) showed a more significant decrease of SGPT level in Rattus norvegicus (Wistar strain) inoculated by Plasmodium berghei as against those in the dose 300 mg/kgBW.Â
Orthopaedic complications of falls in elderly patients: a brief review
Authors: Suyasa I Ketut 
Published: April 2020

Falls are frequent and often cause morbidity, mortality, and disability that require regular nursing. Most of these falls are associated with one or more identifiable risk factors, such as weakness, unsteady gait, confusion and medication use. Usually, it occurs due to a combination of multiple risk factors, such as imbalance, degenerative diseases, porotic bone, and metabolic disturbances. Though in the orthopaedic field, falls will most often result in injury such as femoral neck and proximal humerus fractures, all other parts of the body may also be affected due to various mechanisms of injury. As a disabling phenomenon, fractures due to falls in elderly may then proceed to series of complications, from early to late complications, from local (such as nonunion, muscle atrophy, disuse osteoporosis, etc) to systemic complications (such as pressure sores, pneumonia, ulcers, urinary tract infection, ileus, etc).Â Therefore, prevention will play a critical role in this particular situation.
Physiological peculiarities of thrombocyte activity of candidates into masters of sports in athletics of preadult age
Authors: Medvedev Ilya Nikolayevich 
Published: April 2020

Introduction: Physiologically, platelets are an important component of homeostasis of the whole body and blood system. However, not everything is clear with platelet activity of young healthy persons who have systematic exercises, particularly masters of sportsâ€™ candidate. Its in vitro and in vivo should be studied further.Aim: The aims of this investigation are to find out the abnormalities of thrombocyte activity of master of sportsâ€™ candidate in athletics of young age and compare them with thrombocyte level of young people who avoided exercises during their lifetime. Â Through modulation of their activity, they increase capillaries' perfusion and thereby metabolism in tissues.Materials and methods: In our investigation group we took 125 candidates into masters of sports in athletics of preadult age regularly exercising and participating in competitions of different levels. Control group was consisted of 141 healthy youths having avoided exercises during their lifetime. Biochemical, hematological and statistical methods are applied.Results: Athlete were found to have stable normal functional platelet activity. For subjects between 18 and 22 years, their platelets aggregation was on low level and had no reliable variations related to their stable low sensitivity to inductors. Decreased platelet activity causes conservation in athletes' blood of some quantity of circulating thrombocyte aggregates. It makes positive impact on microcirculation in organs of athlete having regular athletic exercises. Avoidance of exercise in young age promotes gradual rise of thrombocyte activity. Masters of sportsâ€™ in athletics candidate who had intensive muscle activities are characterized by persistence of stable low platelet activity. It provides low quantity of active platelets and their circulating aggregates in blood. Those young persons, who avoided exercises, are noted to have elevated platelet activity with a tendency increased aggregate formation.Conclusion: Masters of sports in athleticsâ€™ candidate doing intensive muscle activity are characterized by preservation of stable not high platelet activity caused by low content of active platelets and their circulating aggregates. Avoiding exercises in young people are noted to have caused the increase of platelet activity with a tendency to cause platelet aggregation.
Correlation between the level of interleukin-6 serum and blood peripheral leukocyte in patients with severe traumatic brain injury
Authors: Bangun Edwin Calvin , Prasetyo Eko , Oley Maximillian Christian 
Published: April 2020

Background: Increased level of interleukin 6 (IL-6) in patients with severe traumatic brain injury (TBI) has been documented, yet studies of its ability to predict the outcome based on the Leukocyte is limited and lacks clarity. This study aims to evaluate the relationship between serum level of IL 6 and Leukocyte among patients with severe TBI.Methods: Twenty patients admitted consecutively to the emergency room (ER) with severe TBI were included in this observational, cross-sectional, single-centre study. Venous blood sample for IL 6 and leukocyte was performed less than 24 hours of trauma in the Intensive Care Unit.Â Age and gender were also recorded. Variable selection was run in stepwise forward fashion. Correlational odds models were conducted to assess how change in Leukocyte is related to levels of IL 6. Data were analysed using SPSS version 17 for Windows.Results: The average age of respondents was 32.45Â±10.71 years old and mostly males (85%). The Glasgow Coma Scale (GCS) was predominant in score 8 (45%). The mean value of IL-6 was 22.00Â±4.66 pg/ml and 22.53Â±8.11 x 103 in leucocyte. The weak insignificant correlation was found between IL-6 and blood peripheral leucocyte using Pearson Correlation test (r = - 0.290; p = 0.108)Conclusion: The recent findings suggest that there is no correlation between IL 6 levels and Leukocyte in patients with severe TBI.
Intradermal suture effect using polypropylene materials on post-operative scar tissue in cases of lower extremities closed fracture cases
Authors: Ario Decky , Wihasetyoko Herman Yosef Limpat , Susilo Hery 
Published: April 2020

Introduction: Hypertrophic scarring is the most common complication in postoperative wounds. One of the preventive measures for scarring is by adding intradermal sutures when closing the surgical wound. This study aims to prove that the addition of intradermal sutures using polypropylene materials can reduce the complications of scarring in postoperative wounds on lower extremities closed fracture cases under internal fixation procedure.Method: Experimental studies using Randomized Controlled Trial Post Test Only Design were carried out in patients with lower extremity fractures in Saiful Anwar General Hospital, Malang. The sample was chosen by proportional sampling which was the group given the treatment as well as the control group (n = 36). The variable measured was the clinical appearance of scar tissue formed 6 months after surgery using the Vancouver Scar Scale.Result: The results showed that the addition of intradermal sutures using polypropylene materials had a significant effect on the formation of postoperative scar tissue.Â The total Vancouver Scar Scale score has a value of p = 0.000 (Î± = 5%) for Mann Whitney test.Conclusion: The conclusion of this study is that the addition of intradermal sutures using polypropylene threads in cases of closed fractures with internal fixation can reduce the appearance of postoperative scar tissue.
The 3rd Live Surgery Workshop International Society of Reconstructive Urology (ISORU), 2nd International Live Surgery Workshop Indonesia Genitourinary Reconstructive Society (InaGURS), and 12th Malang Continuing Urology Education (MCUE)
Authors: Satyagraha Paksi 
Published: April 2020

Dear colleagues and friends,It is truly a pleasure to invite you to actively participate in the upcoming meeting,Â The 3rd Live Surgery Workshop International Society of Reconstructive Urology (ISORU), 2nd International Live Surgery Workshop Indonesia Genitourinary Reconstructive Society (InaGURS), in conjunction with 12thÂ Malang Continuing Urology Education (MCUE), which will be held onÂ September 5th-7th, 2019, in Bali Nusa Dua Convention Center (BNDCC), Bali, Indonesia.The theme chosen is Reconstructive Urology.These three important meetings that held in conjunctionally will provide a wonderful forum for you to refresh your knowledge and explore new techniques and innovation in urology field from well-known experts. The symposia will be held on the first day and on the 2ndÂ and 3rdÂ day, they are going to be the highlight of live surgeries on various cases of reconstructive urology.Additionally, we would like to also invite you to submit your recent achievement in scientific writing in various field of Urology. The scientific committee will prepare the prestigious oral presentation and poster presentation awards.The island of the gods, Bali, awaits you!Undisputable world well-known meeting destination is easily reach with direct flights from most major cities in the world. Since March 2016 Indonesian government has granted 169 countries for FREE Visitor Visa. Please check the list of countries and further details in visa section at the congress website www.mcue.orgPlease make sure you have blocked this important dates and visit the congress website for further program update.See you soon in Baliâ€¦â€¦Â Paksi SatyagrahaCongress Chairman