bluuebunny/crossref_metadata_2025_split huggingface dataset:
XPF Expression And Its Relationship With The Risk And Prognosis Of Colorectal Cancer
Authors: Hu Huixin , Jing Jingjing , Lu Xiaodong , Yuan Yuan , Xing Chengzhong 
Published: October 2020

Abstract
Background: XPF is a key factor contributing to DNA damage excision of nucleotide excision repair pathway. The relationship between XPF expression and the risk and prognosis of colorectal cancer (CRC) is unclear. Methods: In this experiment, a total of 824 cases of colorectal tissue were collected. XPF protein expression was detected by immunohistochemical staining. In order to explore the differential expression of XPF between CRC and non-cancer controls, and the correlation between XPF expression and CRC clinicopathological parameters, we conducted a non-parametric test. Univariate and multivariate Cox regression analyses were conducted to investigate the relationship between XPF expression and CRC prognosis. The Java based software GSEA as well as STRING, David, GO, KEGG were used to explore the function and regulation network of XPF. Results: The results demonstrated that the XPF expression in CRC was significantly up-regulated compared with non-tumor controls (P < 0.001) and adenoma tissues (P < 0.001). XPF protein was highly expressed in the dynamic sequence of anal diseases to adenoma tissues to CRC. Expression of XPF was related to tumor location (P=0.005) and with tumor growth pattern (P=0.009). The results of prognosis analysis suggested that in individuals with T1-2 invasive extent, XPF low expression may be significantly associated with better overall survival (HR = 7.978, 95% CI: 1.208-52.673, P = 0.031). XPF and its interacting genes played a vital role in different processes of nucleotide excision repair pathway. XPF expression was related with Ubiquitin like protein specific protease activity.Conclusions: XPF might be a promising biomarker for CRC risk, and also showed potential as a prognostic predictor in CRC patients.
Impaired Myeloid-derived Suppressor Cells Are Associated With Recurrent Implantation Failure: A Case-Control Study
Authors: Jiang Huanhuan , Zhu Mengting , Guo Peipei , Bi Kaihuan , Lu Zhimin , Li Caihua , Zhai Muxin , Wang Kangxia , Cao Yunxia 
Published: October 2020

Abstract
Background: Studies have reported that myeloid-derived suppressor cells (MDSCs) contribute to maintain pregnancy. The aim of this case-control study was to test whether there is a dysregulation of peripheral MDSCs in recurrent implantation failure (RIF).Methods: 26 RIF patients and 30 controls were recruited. Flow cytometry was applied to characterize polymorphonuclear (PMN)-MDSCs, monocytic-MDSCs (M-MDSCs), effector T cells (Teffs) and regulatory T cells (Tregs) in blood. ELISA was used to define MDSCs correlative cytokines and chemokines in serum from all patients.Results: Compared with controls, RIF patients showed significant reductions of blood PMN-MDSCs, M-MDSCs, Tregs and NO production by PMN-MDSCs, whereas the expression of ζ chain on CD4+T cell receptor (TCR) and CD8+TCR displayed a remarkable upregulation in RIF patients. Moreover, RIF patients presented a lower concentration of serum chemokine (C-C motif) ligand (CCL) 5 and transforming growth factor (TGF)-β than those from controls. Furthermore, the level of TCR ζ chain on CD4+ and CD8+ Teffs was negatively correlated not only with the percentage of PMN-MDSCs, but also with the amount of NO produced by PMN-MDSCs. The frequency of PMN-MDSCs had positive correlations with the concentration of CCL5 and TGF-β.Conclusions: This study indicated that the dysregulation of MDSCs might impair maternal-fetal immune balance thus resulting in RIF.
Identification and Validation of Potential Hub Genes Correlated With Ulcerative Colitis Through Integrated Bioinformatics Analysis
Authors: Li Ting , Hui Wenjia , Halike Halina , Gao Feng 
Published: October 2020

Abstract
Background: Ulcerative colitis (UC) is a chronic nonspecific intestinal inflammatory disorder associated with continuous, diffuse inflammatory alterations in the colonic mucosa of unknown etiology, Increasing evidence has showed aberrant expression of gene plays a vital function in the pathophysiological mechanisms of ulcerative colitis, Herein, we employed bioinformatics to investigate the core of the pathogenesis and provide potential markers for UC. Results: We downloaded the GSE36807, GSE65114, GSE59071 datasets from the Gene Expression Omnibus(GEO), then the differentially expressed genes (DEGs) were determined using adjusted P＜0. 05 and |log2FC>2 | between normal samples and UC samples. Intersection analysis among three datasets showed 12 DEGs were found to be significantly dysregulated in UC. Results indicated that the DEGs were primarily associated with functions like the humoral immune response, antimicrobial humoral response, and CXCR chemokine receptor binding, and they were primarily enriched in KEGG pathways, including the IL−17 signaling pathway, and Toll−like receptor signaling pathway . Cytoscape software calculated that CXCL8, DMBT1, REG3A, S100A8, DUOX2, and MMP1 were hub genes of UC. In addition, We collected samples of 8 UC tissues and 8 normal Colonic tissues to validate the selected genes by mRNA microarray.Conclusions: These results may provide potential biomarkers for UC, and our data and methodology provides new ideas that may be helpful in the understanding of the vital mechanisms underlying UC development.
Barriers to Diabetes Self-Management in New Zealand Patients with Type 2 Diabetes and Poor Glycaemic Control
Authors: Chepulis Lynne , Morison Brittany , Cassim Shemana , Norman Kimberley , Keenan Rawiri , Paul Ryan , Lawrenson Ross 
Published: October 2020

Abstract
Background: Despite the fact that there is an increasingly effective armoury of medications to treat diabetes many patients continue to have substantially elevated blood glucose levels. The purpose of this study was to explore what the barriers to diabetes management are in a cohort of people with diabetes and poor glycaemic control.Methods: Qualitative semi-structured interviews were carried out with 10 people with diabetes who had known diabetes and a recent HbA1c of > 11.3% (100 mmol/mol) to explore their experiences of barriers to diabetes self-management and glycaemic control. Results: Barriers to diabetes management were based around two key themes: biopsychosocial factors and disease awareness / information delivery. Specifically, financial concerns, social stigma, medication side effects and cognitive impairment due to hyperglycaemia were commonly reported as barriers to medication use. Other barriers included a lack of knowledge about their own disease, poor relationships with health care professionals and a lack of relevant resources to support diet and weight loss.Conclusion: people with diabetes with poor glycaemic control experience many of the same barriers as those reported elsewhere, but also experience issues specifically related to their severe hyperglycaemia. Management of diabetes could be improved via the increased use of patient education and availability of locally relevant resources.
Probiotics Mediated Gut Microbiota Diversity Shifts Are Associated With Reduction in Histopathology and Shedding of Lawsonia Intracellularis
Authors: Muwonge Adrian , Karuppannan Anbu K. , Opriessnig Tanja 
Published: October 2020

Abstract
Background: Clinical intervention during bacterial infections in farm animals such as pigs commonly consists of antimicrobial use. With the rise of antimicrobial resistance and the attempts to reduce the use of antibiotics in food animals, effective alternatives are urgently needed to reduce or even remove pathogens and disease risk. Improving clinical outcomes and overall pig health by using probiotics appears attractive. However, reliable data sets on the efficacy of probiotics are scarce. The obligate intracellular bacterium Lawsonia intracellularis is widespread in pigs and associated with severe enteropathy, mainly in the ileum, commonly resulting in substantial reduction in weight gain. The impact of three in-feed probiotics and a commercial live L. intracellularis vaccine were compared in a pig challenge model. Probiotic treatment was associated with reduced L. intracellularis fecal shedding and reduced gut lesions. Here, the bacterial microbiota of the ileum of these pigs was characterized with 16S rRNA gene sequencing and was subsequently analyzed with bioinformatics tools. Results: The greatest microbial richness was observed in the probiotic treated group T03-LAW, which accounted for 87% of richness observed in the study. Treatment had a significant impact on both the microbiota structure and taxonomic profile in the ileum, explaining between 26-36% of the structural variation, with the strongest association in the T03-LAW group. Overall, the largest changes were observed for the pigs treated with in-feed Bacillus pumilus; the microbiota of these pigs had the greatest diversity and highest richness. We also observed depleted and enriched core microbiota amongst the groups; however, there was no correlation with clinical characteristics. The results suggest that an increased diversity of the ileal microbiota is associated with a reduction in shedding i.e. a unit increase in Shannon diversity index resulted in 2.8 log reduction in shedding.Conclusions: Probiotic supplementation of a base feed ration increased ileum microbiota diversity leading to a mitigation of the effects of a pathogenic L. intracellularis challenge. An even and diverse microbiota community benefits pigs infected with L. intracellularis, however, investigations are needed to determine if this is also true for other pathogens. The study unambiguously demonstrates the usefulness of probiotic supplementation in reducing the impact of enteric pathogens and pathogen shedding rates in food animals without the use of antimicrobials.
Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability
Authors: Weber Gregory M. , Birkett Jill , Martin Kyle , II Doug Dixon , Gao Guangtu , Leeds Timothy D. , Vallejo Roger L. , Ma Hao 
Published: October 2020

Abstract
Background: Transcription is arrested in the late stage oocyte and therefore the maternal transcriptome stored in the oocyte provides nearly all the mRNA required for oocyte maturation, fertilization, and early cleavage of the embryo. The transcriptome of the unfertilized egg, therefore, has potential to provide markers for predictors of egg quality and diagnosing problems with embryo production encountered by fish hatcheries. Although levels of specific transcripts have been shown to associate with measures of egg quality, these differentially expressed genes (DEGs) have not been consistent among studies. The present study compares differences in select transcripts among unfertilized rainbow trout eggs of different quality based on eyeing rate, among two year classes of the same population (A1, A2) and a population from a different hatchery (B). The study compared 65 transcripts previously reported to be differentially expressed with egg quality in rainbow trout. Results: There were 32 transcripts identified as DEGs among the three groups by regression analysis. Group A1 had the most DEGs, 26; A2 had 15, 14 of which were shared with A1; and B had 12, 7 of which overlapped with A1 or A2. Six transcripts were found in all three groups, dcaf11, impa2, mrpl39_like, senp7, tfip11 and uchl1. Conclusions: Our results confirmed maternal transcripts found to be differentially expressed between low- and high-quality eggs in one population of rainbow trout can often be found to overlap with DEGs in other populations. The transcripts differentially expressed with egg quality remain consistent among year classes of the same population. Greater similarity in dysregulated transcripts within year classes of the same population than among populations suggests patterns of transcriptome dysregulation may provide insight into causes of decreased viability within a hatchery population. Although many DEGs were identified, for each of the genes there is considerable variability in transcript abundance among eggs of similar quality and low correlations between transcript abundance and eyeing rate, making it highly improbable to predict the quality of a single batch of eggs based on transcript abundance of just a few genes.
Multicenter Randomized Controlled Trial Comparing Digital and Traditional Chest Drain in a VATS&nbsp;Pulmonary Lobectomy Cohort: Interim Analysis
Authors: Mendogni Paolo , Tosi Davide , Marulli Giuseppe , Comacchio Giovanni Maria , Pieropan Sara , Rossi Veronica , Brascia Debora , Andriolo Luigi Gaetano , Imbriglio Giovanna , Bonitta Gianluca , Lopez Camillo , Rea Federico , Nosotti Mario 
Published: October 2020

Abstract
Background: The usefulness of digital chest drain is still discussed. We are carrying out a study to determine if the use of a digital system compared with a traditional system reduces the duration of chest drainage. To evaluate safety, benefit, or futility of this trial we planned the current interim analysis.Methods: An interim analysis on preliminary data from ongoing investigator-initiated, multicenter, interventional, prospective randomized trial. Original protocol number: (NCT03536130). The interim main endpoint was overall complications; secondary endpoints were the concordance between the two primary endpoints of the RCT (chest tube duration and length of hospital stay). We planned the interim analysis when half of the patients have been randomised and completed the study. Data were described using mean and standard deviation or absolute frequencies and percentage. T-test for unpaired samples, Chi-square test, Poisson regression and absolute standardized mean difference (ASMD) were used. P-value <0.05 was considered significant. Results: From April 2017 to November 2018, out of 317 patients were enrolled by 3 centers, 231 fulfilled inclusion criteria and were randomized. Twenty-two of them dropped out after randomization. Finally, 209 patients were analyzed: among them 94 used the digital device and 115 the traditional one. The overall postoperative complications were 35 (16.8%) including prolonged air leak (1.9%). Mean chest tube duration was 3.6 days (SD=1.8), with no differences between two groups (p=0.203). The overall difference between hospital stay and chest tube duration was 1.4 days (SD=1.4). Air leak at first postoperative day detected by digital and traditional devices predicted increasing in tube duration of 1.6 day (CI 95% 0.8-2.5, p<0.001) and 2.0 days (CI 95% 1.0-3.1, p<0.001), respectively. Conclusions: This interim analysis supported the authors’ will to continue with the enrollment and to analyze data once the estimated sample size will be reached.Trial registration: Trial registration number NCT03536130, Registered 24 May 2018 - Retrospectively registered, https://clinicaltrials.gov/ct2/results?cond=&term=NCT03536130&cntry=&state=&city=& dist=
Treatment of Intrathoracic Anastomotic Leak After Esophagectomy with the Sump Drainage Tube
Authors: Yin Qifan , Zhou Shaohui , Song Yongbin Song , Xun Xuejiao Xun , Liu Nana Liu , Liu Lijun 
Published: October 2020

Abstract
Background: Intrathoracic esophagogastric anastomotic leak is one of the deadliest complications after esophagectomy. In recent years, we have implemented new method for the treatment of intrathoracic esophagogastric anastomotic leak with the nasogastric placement of sump drainage tube through fistula into abscess cavity. The aim of this study is to compare the efficacy of the new method and conventional therapies for intrathoracic anastomotic leak after esophagectomy. Method: Esophagectomy and esophagogastric anastomotic procedures were performed in 875 patients at our institution from January 2008 to December 2019. Of these patients, 43(4.9%) patients developed intrathoracic anastomotic leaks postoperatively were enrolled into our study and their clinical data were retrospectively assessed. 20 (47%) patients from January 2008 to December 2012 received conventional treatments (group 1) known as the traditional ‘‘three-tube method’’, and 23 (53%) patients from January 2013 to December 2019 received new treatments (group 2), consisted of conventional therapies and the nasogastric placement of sump drainage tube through fistula into abscess cavity. Results: The presence of intrathoracic anastomotic leak was proven by contrast esophagography in 43 patients (4.9%). Among them, The average duration of chest tube was 47 days in group 1 and 28 days in group 2. The average length of leak treatment was 52 days in group 1 and 35 days in group 2. The average length of postoperative hospital stay was 56 days in group 1 and 39 days in group 2,respectively. 7(35%) patients among 20 patients died from intrathoracic anastomotic leak in group 1;and 3(13%) patients among 23 patients died from intrathoracic anastomotic leak in group 2. Compared with the conventional treatments (group 1), The average duration of chest tube was significantly decreased in the new treatments (group 2) (P < 0.01), as well as the length of leak treatment (P < 0.05) and the length of postoperative hospital stay (P < 0.01). However, there was no significant difference in the mortality rate (P=0.148 > 0.05).Conclusion: In conclusion, Our results showed this method of the nasogastric placement of sump drainage tube through fistula appears to be an safe, effective, technically feasible treatment option for intrathoracic esophagogastric anastomotic leak. The efficacy and feasibility could be further investigated with a well-designed and large-scale RCT research.
Perioperative outcomes of combined heart surgery and lung tumor resection: A systematic review and meta-analysis
Authors: Cheng Shizhao , Jiang Yiyao , Li Xin , Lu Xike , Zhang Xun , Sun Daqiang 
Published: October 2020

Abstract
Objective: The coexistence of concomitant lesions of the heart and lungs needed surgical intervention is increasing. Simultaneous cardiac surgery with pulmonary resection can solve the lesions at the same time, thus avoiding the second operation. However, concern exists regarding the potentially increased mortality and complication rate of simultaneous surgery and the adequacy of lung exposure during heart surgery. Therefore, we performed a meta-analysis to evaluate the perioperative mortality and complication rate of combined heart surgery and lung tumor resection.Methods: A comprehensive literature search was performed in July 2020. PubMed, Embase and Web of Science databases were searched to collect studies reported the perioperative outcomes of combined heart surgery and lung tumor resection. Two reviewers independently screened literatures, extracted data and assessed the risk of bias of included studies. Pooled proportion and its 95% confidence intervals (95% CI) was performed by R version 3.6.1 using the meta package.Results: A total of 536 patients from 29 studies were included in this analysis. Overall, the results of this meta-analysis showed that the pooled proportion of operative mortality was 0.01 (95% CI: 0.00, 0.03) and the pooled proportion of postoperative complications was 0.40 (95% CI: 0.24, 0.57) for patients underwent combined cardiothoracic surgery. Subgroup analysis by lung pathology revealed that, for lung cancer patients, the pooled proportion of anatomical lung resection was 0.99 (95% CI: 0.95, 1.00), and the pooled proportion of systematic lymph node dissection or sampling was 1.00 (95% CI: 1.00, 1.00). Subgroup analysis by heart surgery procedures found that the pooled proportion of postoperative complications of coronary artery bypass grafting (CABG) patients using off-pump method was 0.17 (95% CI: 0.01, 0.43), while the pooled proportion of on-pump method was 0.61 (95% CI: 0.38, 0.82).Conclusion: This study presented that combined heart surgery and lung tumor resection had a low mortality rate and an acceptable complication rate. Subgroup analysis revealed most lung cancer patients underwent uncompromised anatomical resection and mediastinal lymph node sampling or dissection during combined cardiothoracic surgery, and showed off-pump CABG could potentially reduce the complication rate compared with on-pump CABG. While further researches are still needed.
Giant Intrapericardial Bronchogenic Cyst Associated with Congestive Heart Failure and Atrial Fibrillation: A Case Report
Authors: Maldonado Javier , Molina German , T Francisco M- Rincón , Buitrago Lina M. Acosta , Rivera Carlos J- Perez 
Published: October 2020

Abstract
Background: Large intracardiac bronchogenic cysts are rare mediastinal masses, however they must always be considered in the differential diagnosis of heart failure. Case Presentation: We present a 60-year-old female patient with de novo atrial fibrillation and heart failure, resulting from an incidental large intrapericardial mass. The patient underwent successful surgical resection, with pathological findings confirming a bronchogenic cyst.Conclusions: Large bronchogenic cysts located intrapericardially are very rare, however they should be included in the differential diagnosis of patients presenting with atrial fibrillation and heart failure.
Automatic Identification of Upper Extremity Rehabilitation Exercise Type and Dose Using Body-Worn Sensors and Machine Learning: A Pilot Study
Authors: Balestra Noah , Sharma Gaurav , Riek Linda M. , Busza Ania 
Published: October 2020

Abstract
Background: Prior studies suggest that participation in rehabilitation exercises improves motor function post-stroke; however, studies on optimal exercise dose and timing have been limited by the technical challenge of quantifying exercise activities over multiple days. Objective: In this exploratory study, we assessed the feasibility of using body-worn sensors to track rehabilitation exercises in the inpatient setting and investigated which recording parameters and data analysis strategies are sufficient for accurately identifying and counting exercise repetitions. Methods: MC10 BioStampRC® sensors were used to measure accelerometry and gyroscopy data from arms of healthy controls (n=13) and patients with upper extremity (UE) weakness due to recent stroke (n=13) while the subjects performed three pre-selected UE exercises. Sensor data was then labeled by exercise type, and this labeled data set was used to train a machine learning classification algorithm for identifying exercise type. The machine-learning algorithm and a peak-finding algorithm were used to count exercise repetitions in non-labeled data sets. Results: We achieved a repetition counting accuracy of 95.6 ± 2.4 % overall, and 95.0 ± 2.3 % in patients with UE weakness due to stroke. Accuracy was decreased when using fewer sensors or using accelerometry data alone. Conclusions: Our exploratory study suggests that body-worn sensor systems are technically feasible, well-tolerated in subjects with recent stroke, and may ultimately be useful for developing a system to measure total exercise “dose” in post-stroke patients during clinical rehabilitation or clinical trials.