bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Pediatric Influence of Cooling Duration on Efficacy in Cardiac Arrest Patients (P-ICECAP): Statistical Methods Planned in the Bayesian, Adaptive, Duration Finding Trial
Authors: VanBuren John M. , Yeatts Sharon D. , Holubkov Richard , Moler Frank W. , Topjian Alexis , Page Kent , Clevenger Robert G. , Meurer William J. 
Published: December 2024

Objectives:
To determine the optimal cooling duration for children after out-of-hospital cardiac arrest (OHCA) using an adaptive Bayesian trial design.

Design:
The Pediatric Influence of Cooling duration on Efficacy in Cardiac Arrest Patients (P-ICECAP) trial is a randomized, response-adaptive duration/dose-finding clinical trial with blinded outcome assessment. Participants are randomized to one of several cooling durations (0, 12, 18, 24, 36, 48, 60, 72, 84, or 96 hr). The first 150 participants are randomized 1:1:1 to 24-, 48-, and 72-hour durations. Response-adaptive randomization is used thereafter to allocate participants based on emerging duration-response data.

Setting:
PICUs.

Patients:
Up to 900 pediatric patients 2 days to younger than 18 years old who have survived OHCA and been admitted to an ICU.

Interventions:
Duration of targeted temperature management using a surface temperature control device.

Measurements and Main Results:
The primary outcome is the Vineland Adaptive Behavior Scales-Third Edition mortality composite score, assessed at 12 months. Secondary outcomes include changes in the Pediatric Cerebral Performance Category and Pediatric Resuscitation after Cardiac Arrest scores, as well as survival at 12 months. Bayesian modeling is employed to evaluate the duration-response curve and determine the optimal cooling duration. The trial is designed to adaptively update randomization probabilities every 10 weeks, maximizing the allocation of participants to potentially optimal cooling durations. Over 90% power is achieved for the hypothesized scenarios.

Conclusions:
The P-ICECAP trial aims to identify the shortest cooling duration that provides the maximum treatment effect for pediatric OHCA patients. The adaptive design allows for flexibility and efficiency in handling various clinical scenarios, potentially transforming pediatric cardiac arrest care by optimizing hypothermia treatment protocols.
Sagittal Split Ramus Osteotomy Without Segmental Fixation in Skeletal Class II
Authors: Narahara Shun , Shido Rena , Koga Takamitsu , Moriuchi Emi , Yamada Tomohiro , Ohba Seigo , Shirota Tatsuo 
Published: December 2024

The new orthognathic treatment strategy of non-segmental fixation (non-fix) sagittal split ramus osteotomy (SSRO) with jaw exercise initiation on the second postoperative day, the so-called physiological positioning strategy (PPS), induces good skeletal stability with few temporomandibular joint (TMJ) symptoms after surgery in skeletal class III. This study aimed to clarify whether non-fix SSRO with modified PPS can be applied to skeletal class II. This retrospective study included skeletal class II patients who underwent non-fix SSRO to correct mandibular retrognathia. Jaw exercise was initiated within 10 postoperative days. Cephalometric analyses were performed before (T1), immediately after (T2), and more than 6 months after (T3) surgery. Skeletal and dental stability, and TMJ symptoms were evaluated. Twelve patients (2 men, 10 women; mean age 22.9±7.1 y) were included. The mean mandibular advancement was 6.8±1.8 mm. angle between S-N and N-B. was 73.6±4.5, 77.1±3.9, and 75.1±4.4 degrees at T1, T2, and T3, meaning 60.5% relapse. The Menton moved forward by 6.3 mm from T1 to T2 and relapsed by 4.6 mm at T3. The duration of intermaxillary traction showed a moderate negative correlation with angle between S-N and N-B. relapse (r=−0.42, P=0.1787). Only 2 TMJ symptoms (2/24, 8.3%) developed postoperatively. This study suggests that non-fix SSRO with modified PPS is a suitable method for skeletal class II malocclusion. Further research with a larger sample size and detailed analysis of the effect of intermaxillary traction on skeletal stability after surgery is needed.
Compound Danshen Yeast 1.0
Authors: Li Rongsheng , Wang Jie , Han Yuanyuan , Dai Zhubo 
Published: December 2024

Abstract

Background
Heterologous synthesis presents a promising new approach for accessing the active ingredients of traditional Chinese medicine (TCM), contributing to the conservation of natural medicinal resources. Compound Danshen preparation, a widely used TCM formulation, is designed to treat coronary heart disease and angina pectoris. It is primarily composed of Salviae Miltiorrhizae Radix et Rhizoma (Danshen), Notoginseng Radix et Rhizoma (Sanqi), and borneol (Bingpian). Danshen primarily yields tanshinones and phenolic acids, while Sanqi produces notoginsenosides. Borneol serves as an auxiliary agent to promote mental clarity, dissipate heat, and relieve pain.

Objectives
The objective is to employ heterologous synthesis in a single yeast strain to produce the active ingredients of Compound Danshen preparation, and these include notoginsenosides, tanshinones, and borneol.

Methods
Firstly, the “plug-and-play” terpene synthase screening framework in Saccharomyces cerevisiae was used for the identification of a novel, highly efficient bornyl diphosphate synthase. Furthermore, by leveraging the Compound Danshen preparation as a basis to concurrently reconstruct the biosynthetic pathways for the notoginsenoside precursor protopanaxadiol (a triterpene), the tanshinone precursor miltiradiene (a diterpene), and borneol (a monoterpene) within a single yeast strain.

Results
This engineered strain, termed Compound Danshen Yeast 1.0, successfully produced protopanaxadiol at 62.34 mg/L, miltiradiene at 15.38 mg/L, and borneol at 2.54 mg/L in shake-flask fermentation.

Conclusions
This research signifies the inaugural cross-species and multicomponent synthetic biology endeavor that enables the synthesis of active ingredients in engineered cells, setting the stage for the industrial manufacture of TCM compounds.
UTX Epigenetically Imposes a Cytolytic Effector Program in Autoreactive Stem-like CD8+ T cell Progenitors
Authors: Chen Ho-Chung , Wang Hsing Hui , Kohn Lisa A. , Sailer David , Zhang Shirley , McCarthy Ethan , Seyedsadr Maryam , Zhou Zikang , Yin Xihui , Wilkinson Nicole , Ortega Jessica , Lechner Melissa G. , Hugo Willy , Su Maureen A. 
Published: December 2024

SummaryType 1 Diabetes Mellitus (T1D) is an autoimmune disease caused by unremitting immune attack on pancreas insulin-producing beta cells. Persistence of the autoimmune response is mediated by TCF1+ Ly108+ progenitor CD8+ T (Tprog) cells, a stem-like population that gives rise to exhausted effectors with limited cytolytic function in chronic virus infection and cancer. What paradoxically drives Tprogconversion to highly cytolytic effectors in T1D, however, remains unclear. Here, we show that the epigenetic regulator UTX controls diabetogenic CD8+ Tprogdifferentiation by poising chromatin for transition to a cytolytic effector state. Indeed, deletion of UTX function in T cells impairs conversion of Tprogto autoimmune effectors and protects mice from spontaneous diabetes, as well as an aggressive form of autoimmune diabetes induced by anti-PD1 cancer immunotherapy. Furthermore, short-term treatment with UTX inhibitor GSKJ4 similarly protects from T1D, highlighting the therapeutic potential of targeting UTX-mediated mechanisms to break unremitting autoimmune responses.
Start&Stop - a PhysiCell and PhysiBoSS 2.0 add-on for interactive simulation control
Authors: Smeriglio Riccardo , Bardini Roberta , Savino Alessandro , Di Carlo Stefano 
Published: December 2024

AbstractIn computational biology,in silicosimulators are vital for exploring and understanding the behavior of complex biological systems. Hybrid multi-level simulators, such asPhysiCellandPhysiBoSS 2.0, integrate multiple layers of biological complexity, providing deeper insights into emergent patterns. However, one key limitation of these simulators is the inability to adjust simulation parameters once they have started, which impedes real-time exploration and adaptation of dynamic protocols—ranging from biofabrication toin vitropharmacological testing. To address this challenge, we introduce theStart&Stopadd-on forPhysiCell, which is automatically adaptable toPhysiBoSS 2.0. This add-on offers multi-level state preservation and multi-modal stop control—triggered by simulation time or cell conditions—enabling users to pause a simulation, adjust parameters, and then resume from the exact halted state. We validateStart&Stopusing a well-establishedPhysiBoSS 2.0tumor spheroid 3T3 mouse fibroblasts use case under tumor necrosis factor (TNF) stimulation, demonstrating that it preserves the simulator’s original behavior while enabling interactive, real-time configuration changes that facilitate the exploration of diverse and adaptive treatment strategies. By enhancing flexibility and user interaction,Start&StopmakesPhysiCellandPhysiBoSS 2.0more akin to realin vitroscenarios, thus expanding the range of potential simulations and advancing more effective protocol development in a variety of applications.
Single-port laparoscopic surgery for sigmoid colon cancer: a novel surgical strategy based on “peritoneal dome”
Authors: Qijun Lv , Jin Tang , Pengcheng Ye , Jun Shen , Qinyuan Wang , Jingjing Chen , Hongshen Huang , Hailin Gan , Junyi Li , Ruijie Lin , Yuhang Liu , Shoujiang Wei 
Published: December 2024

AbstractAimsThis study aimed to introduce a novel ‘peritoneal dome’ strategy for single-port laparoscopic surgery in patients with sigmoid colon cancer and to evaluate the feasibility and safety of this approach.MethodsA total of 12 patients at the affiliated hospital of North Sichuan Medical College from January 2022 to August 2024 were enrolled. All procedures were performed by the same surgical team. By collecting and analyzing the demographic information, clinical characteristics, surgical outcomes and postoperative recovery indicators of this group of patients, the short-term efficacy and safety of this surgical strategy were preliminarily studied.ResultsThe median age of patients was 72 years (range, 58-81), with a median body mass index (BMI) of 25.8 kg/m2(range, 22–31.2). Most patients had underlying respiratory conditions, particularly chronic obstructive pulmonary disease (COPD) (80%). All patients successfully completed the single-port laparoscopic surgery without conversion to laparotomy. The median of the total operating time was 98.80 minutes. The mean arterial carbon dioxide pressure (PaCO2) during the extraperitoneal phase was significantly lower (32.5 mmHg) compared to the intraperitoneal phase (47.1 mmHg). The median estimated blood loss was 19.20 ml, with no transfusions required and no significant operative complications reported. Pathological analysis revealed that 83.3% of patients had advanced disease (stage III), and the average number of harvested lymph nodes was 18.92. Postoperatively, patients experienced a mean time to ambulation of 1.25 days and a mean hospital stay of 6.17 days. Complications occurred in 33.3% of patients, primarily classified as grade I and II according to the Clavien-Dindo classification. Notably, there were no cases of anastomotic leakage or severe complications.ConclusionThe single-port laparoscopic surgery based on a “peritoneal dome” supported strategy for sigmoid colon cancer demonstrates effective oncological management with minimal complications and a favorable recovery profile. The results suggest that this technique is a viable option for patients with advanced sigmoid colon cancer, particularly in those with significant comorbidities.
TheChlamydomonas reinhardtiiCLiP2 mutant collection expands genome coverage with high-confidence disrupting alleles
Authors: Lunardon Alice , Patena Weronika , Pacini Cole , Warren-Williams Michelle , Zubak Yuliya , Laudon Matthew , Silflow Carolyn , Lefebvre Paul , Jonikas Martin 
Published: December 2024

AbstractChlamydomonas reinhardtii(Chlamydomonas hereafter) is a powerful model organism for studies of photosynthesis, ciliary motility, and other cellular processes [1–4]. The CLiP library of mapped nuclear random insertion mutants [5,6] has accelerated progress for hundreds of laboratories in these fields by providing mutants in genes of interest. However, its value was limited by its modest coverage of the genome with high-confidence disruption alleles (46% of nuclear protein-coding genes with 1+ high-confidence allele in exons/introns; 12% of genes with 3+ alleles in exons/introns). Here we introduce the CLiP2 (Chlamydomonas Library Project 2) library, which greatly expands the number of available mapped high-confidence insertional mutants. The CLiP2 library includes 71,700 strains, covering 79% of nuclear protein-coding genes with 1+ high-confidence allele in exons/introns and 49% of genes with 3+ alleles in exons/introns. The mutants are available to the community via the Chlamydomonas Resource Center.
Financial literacy and its determinants of self-employed workers in the Ghanaian economy
Authors: Ofori Edmond 
Published: December 2024

PurposeThe main purpose of this study was to investigate the level of financial literacy and its determinants among self-employed workers in the Ghanaian economy.Design/methodology/approachThe study used a non-random sampling technique known as quota sampling for the purpose of selecting respondents from the targeted population. The study used an instrumental variable Tobit regression model to investigate the determinants of financial literacy among self-employed workers in the Ghanaian economy.FindingsThe study found that the level of financial literacy of the self-employed workers in the Ghanaian economy is low. The study also revealed that financial knowledge, financial attitude, financial behaviour, education and age have a statistically significant influence on financial literacy.Practical implicationsThis paper indicates that the level of financial literacy of the self-employed workers in the Ghanaian economy is low and requires self-employed workers in Ghana to attend workshops and do short courses in finance, banking and economics organised by experts in those areas to increase their level of financial literacy.Originality/valueLimited research exists on the level of financial literacy and its determinants among self-employed workers in developing countries. Therefore, I conducted this study to address the research gap in financial literacy by examining the level of financial literacy and the various factors that impact the financial literacy of self-employed workers in Ghana.
Improving gender responsiveness of the cassava value chain in sub-Saharan Africa: the role of CSR in Nigeria’s oil-producing region
Authors: Uduji Joseph Ikechukwu , Okolo-Obasi Nduka Vitalis Elda , Uduji Joy Ukamaka , Emengini Emeka Steve , Odoh Longinus Chukwudi , Okoro Deborah Patience , Okafor Chikodili Nkiruka 
Published: December 2024

Purpose The purpose of this paper is to critically examine the multinational oil companies’ (MOCs) corporate social responsibility (CSR) initiatives in Nigeria. Its special focus is to investigate the impact of the global memorandum of understanding (GMoU) on improving gender responsiveness of the cassava value chain (CVC) in the Niger Delta region of Nigeria.Design/methodology/approach This paper adopts a survey research technique, aimed at gathering information from a representative sample of the population, as it is essentially cross-sectional, describing and interpreting the current situation. A total of 780 rural women respondents were sampled across the Niger Delta region.Findings The results from the use of a combined logit model and propensity score matching indicate that CSR of the MOCs using the GMoU model has recorded little but significant success in enhancing rural women’s participation in the CVC in the Niger Delta.Practical implications This implies that if CSR interventions are not tailored to enhance opportunities for women, they may contribute towards reducing the participation of women in economic, political and social development and, by extension, damping efforts of reducing poverty and achieving sustainable development goals (SDGs) in the Niger Delta.Social implications This suggests that MOCs’ CSR interventions in the CVC should consider gender relations to benefit men and women and alleviate household poverty.Originality/value This research contributes to the inequality debate in the agrifood value chain and inclusive growth literature from the CSR perspective in developing countries and the rationale for demand for social projects by host communities. It concludes that businesses has an obligation to help in solving problems of public concern.
The effect of brand authenticity on brand loyalty in the frozen food industry: findings from PLS-SEM and fsQCA
Authors: Shahzad Saqib , Li Shan , Sarwar Adnan 
Published: December 2024

PurposeThis study aims to investigate the effect of brand authenticity on consumer brand loyalty in the Pakistani frozen food industry sector in the light of the stimulus-organism-response (SOR) theory.Design/methodology/approachThe quantitative approach utilized a survey questionnaire to acquire customers’ perceptions. A simple random technique was used to collect data. About 255 questionnaires were analysed, and the response rate was 72.86%. The measurement and structural model were constructed through Smart PLS-4 and fsQCA.FindingsThe findings indicated that brand authenticity positively affects brand loyalty in the Pakistani frozen food industry. Results further proved that brand involvement has a full mediation effect, fsQCA supports the same results, and customer satisfaction has a partial mediation effect. These findings offer valuable insights into the frozen food industry in Pakistan and other countries, allowing them to create successful tactics to engage customers and foster brand loyalty. The findings reveal the complexity and ever-changing nature of how consumers assess brand authenticity in the frozen food industry.Practical implicationsThese findings also have implications for the frozen food business’s marketing and brand positioning strategies, particularly its utilization of brand authenticity to attract and retain consumers.Originality/valueThis is the first paper in the frozen food sector from the perspective of brand authenticity.
Drivers, inhibiting factors and facilitating factors influencing purchase intention of suboptimal foods among Taiwanese consumers
Authors: Chen Mei-Fang 
Published: December 2024

PurposeTo elucidate the factors influencing suboptimal food (SF) purchase intention among Taiwanese consumers, this study extended the theory of planned behaviour (TPB) model by incorporating driving factors that encourage purchasing of SFs, that is, incorporating food waste awareness and personal norms. The inhibiting moderator of health consciousness and facilitating moderator of price consciousness were also considered.Design/methodology/approachA total of 305 online questionnaire responses were analysed. Moderated regression analysis was performed to test the hypotheses proposed in this study.FindingsConsumer attitudes toward purchasing SFs and perceived behavioural control as well as their food waste awareness and personal norms are determinants of their SF purchase intention. Health consciousness inhibits SF purchase intention, and price consciousness promotes SF purchase intention. In addition, the moderating effect of health consciousness reverses the positive relationship between personal norms and SF purchase intention, turning the relationship negative. However, the moderating effect of price consciousness strengthens the positive relationship between personal norms and SF purchase intention.Originality/valueIn addition to extending the TPB model, this study considered the main effects and moderating effects of health consciousness and price consciousness on consumers’ intention to purchase SF. The research findings contribute to the understanding of the relevant factors that influence Taiwanese consumers’ SF purchase intention. The study also outlines the implications of its findings in terms of encouraging consumers to purchase SFs to reduce food waste.
Attitudes of millennials toward corporate responsibility: a 28-society multilevel analysis
Authors: Terpstra Tong Jane L.Y. , Ralston David A. , Furrer Olivier , Karam Charlotte M. , Egri Carolyn Patricia , Richards Malika , Dabić Marina , Reynaud Emmanuelle , Fu Pingping , Palmer Ian , Srinivasan Narasimhan , de la Garza Carranza Maria Teresa , Butt Arif , Ruiz-Gutiérrez Jaime , Lee Chay Hoon , Naoumova Irina , Moon Yong-Lin , Pla-Barber Jose , Molteni Mario , Kuo Min Hsu , Casado Tania , Sidani Yusuf M. , Mockaitis Audra , Milton Laurie , Zatorska Luiza , Ho Beng Chia , Gelbuda Modestas , Alas Ruth , Danis Wade 
Published: December 2024

PurposeWe examined the attitudes of millennial-aged business students toward economic, social and environmental corporate responsibility (CR). Currently, these individuals are of an age that they have entered the workforce and are now ascending or have ascended into roles of leadership in which they have decision-making power that influences their company’s CR agenda and implementation. Thus, following the ecological systems perspective, we tested both the macro influence of cultural values (survival/self-expression and traditional/secular-rational values) and structural forces (income inequality, welfare socialism and environmental vulnerability) on these individuals’ attitudes toward CR.Design/methodology/approachThis is a multilevel study of 3,572 millennial-aged students from 28 Asian, American, Australasian and European societies. We analyzed the data collected in 2003–2009 using hierarchical linear modeling.FindingsIn our multilevel analyses, we found that survival/self-expression values were negatively related to economic CR and positively related to social CR while traditional/secular-rational values was negatively related to social CR. We also found that welfare socialism was positively related to environmental CR but negatively related to economic CR while environmental vulnerability was not related to any CR. Lastly, income equality was positively related to social CR but not economic or environment responsibilities. In sum, we found that both culture-based and structure-based macro factors, to varying extents, shape the attitudes of millennial-aged students on CR in our sample.Originality/valueOur study is grounded in the ecological systems theory framework, combined with research on culture, politico-economics and environmental studies. This provides a multidisciplinary perspective for evaluating and investigating the impact that societal (macro-level) factors have on shaping attitudes toward businesses’ engagement in economic, social and environmental responsibility activities. Additionally, our multilevel research design allows for more precise findings compared to a single-level, country-by-country assessment.