bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cost-effectiveness analysis for HbA1c test intervals to screen patients with type 2 diabetes based on risk stratification.
Authors: Ohde Sachiko , Moriwaki Kensuke , Takahashi Osamu 
Published: August 2020

Abstract
Background: To determine the best HbA1c test interval strategy for detecting new type 2 diabetes mellitus (T2DM) cases in a healthy population, HbA1c test characteristics, risk stratification towards T2DM and cost effectiveness were considered.Methods: State transition models were built to study the optimal screening interval for new cases of T2DM among each age- and BMI-stratified health population. Age was stratified into 30-44-, 45-59-, and 60-74-year-old age groups, and BMI was also stratified into underweight (<18.5 kg/m2), normal (18.5-25 kg/m2), overweight (25-30 kg/m2) and obese (≥30 kg/m2). In each model, different HbA1c test intervals were compared to evaluate costs per quality-adjusted life year (QALY) and the incremental cost-effectiveness ratio (ICER). We compared intervals annually (current Japanese strategy), every three years (US and UK recommendations) and tailored to each risk stratification group, based on our previous work. All model parameters, including screening and treatment costs, complications and mortality rates and utilities, were applied from published studies. The willingness-to-pay threshold in the cost-effectiveness analysis was set to US $50,000/QALY.Results: The HbA1c test interval for detecting T2DM in a healthy population varies by age and BMI. Three-year intervals were the most cost effective in obese groups at all ages—30-44: $15,034/QALY, 45-59: $11,849/QALY, 60-74: $8,685/QALY—compared with the other two interval strategies. The three-year interval was also the most cost effective in the 60-74-year-old age groups—underweight: $11,377/QALY, normal: $18,123/QALY, overweight: $12,537/QALY—and in the overweight 45-59-year-old group; $18,918/QALY. In other groups, the screening interval for detecting T2DM was found to be longer than three years, as previously reported. Annual screenings were dominated in many groups with low BMI and in younger age groups. Based on the probability distribution of the ICER, QALY does not show much difference among any groups.Conclusions: Annual screening to detect T2DM was not cost effective and should not apply to any population. The three-year screening interval was optimal among all elderly populations, the obese groups of all ages and the overweight 45-59-year-old group. For the low BMI and younger age groups, the optimal HbA1c test interval can be longer than three years.
Liver transplantation in recipients with class III obesity: post-transplant outcomes and weight gain
Authors: Soma Daiki , Park Yujin , Mihaylov Plamen , Ekser Burcin , Ghabril Marwan , Lacerda Marco , Charasani Naga , Mangus Richard Shane , Kubal Chandrashekhar Avinash 
Published: August 2020

Abstract
BackgroundOver the past decades, there has been a dramatic increase in obesity in the United States. Several studies have reported conflicting results for the impact of obesity on outcomes of liver transplantation (LT). This study aims to assess the impact of severe obesity on outcomes of LT and change in body mass index (BMI) after transplantation.MethodsAll adult LT performed between July 2001 and December 2018 were reviewed. A retrospective analysis was conducted. BMI of recipients is subdivided into six categories: underweight, normal, overweight, class Ⅰ obesity, class Ⅱ obesity, and class Ⅲ obesity (<18.5; 18.5-24.9; 25‐29.9; 30‐34.9; 35‐39.9; ≥40 kg/m2, respectively). Survival outcomes were compared between each group. Post-transplant BMI was followed up in a sub-group of patients receiving LT from January 2008 to December 2018.ResultsAmong 2024 patients in the analytic cohort, 1.9% were underweight, 24.5% were normal, 32.6% were overweight, 25% were in class Ⅰ obesity, 9.3% were in class Ⅱ obesity, and 1.1% were in class Ⅲ obesity. There was no significant difference in patient and graft survival at 10-year follow-up with respect to recipient obesity. The 1, 3, 5, and 10-year graft and patient survivals in class Ⅲ obesity group were 97.0%, 92.1%, 87.0%, and 79.8% for patient survival and 94.4%, 85.1%, 79.8%, and 72.5% for graft survival.BMI of all groups except the underweight group declined in the first three months postoperatively. After the three months, BMI of all groups except the class Ⅲ obesity group returned to the pre LT level by two years and reached a plateau by five years. In patients with class Ⅲ obesity, there was a significant increase in body weight after long term follow up.ConclusionIn this study, class Ⅲ obesity is not associated with higher mortality. Obesity, including class Ⅲ obesity, should not be considered to be a contraindication to LT in the absence of other contraindications. Post-LT interventions are required to prevent significant weight gain in recipients with class Ⅲ obesity after transplantation.
Barriers to the Cervical Cancer Screening in the Northern Slovakia
Authors: Svihrova Viera , Kocsis Lukas , Svihra Jan , Szaboova Veronika 
Published: August 2020

Abstract
Background

A barrier to screening is a specific attitude, opinion or state that prevents the patient from seeking preventive care. The aim of this study was to identify and compare barriers to cervical cancer screening (CCS) in Northern Slovakia between women seeking and not seeking CCS.
Methods

Data collection was performed in twenty gynaecological departments, each department sending data from five healthy women and five untreated women with cervical cancer. Women completed a validated and standardized questionnaire with 28 statements (the CPC-28 questionnaire: “Creencias, Papanicolaou, Cancer-28” questionaire – Beliefs about Papanicolaou and Cervical Cancer). A four-point Likert scale (item score from 1 to 4) was used to assess responses. A linear transformation was made to calculate the responses. Differences with a p value of < 0.05 were considered statistically significant.
Results

From the 200 questionnaires, 135 (67.5%) participants were divided into the women seeking CCS (n = 97) and the women not seeking CCS (n = 38). The women not seeking CCS vs seeking CCS had higher barriers according to the CPC-28 domain one (median; interquartile range: 33.33; 28.70-40.74 vs 14.82; 7.41–29.63; p < 0.001). The risk of not seeking CCS was statistically significant in non-working (OR; 95% CI: 2.458; 1.127–5.358; p < 0.024), non-childbearing women (OR; 95% CI: 3.302; 1.421–7.671; p < 0.006) and women without cervical cancer (OR; 95% CI: 4.709; 1.960-11.317; p < 0.001).
Conclusion

We identified barriers to cervical cancer screening in both of our groups but the results were more frequent and statistically significant in the women not seeking CCS.
Discovery of exolytic heparinases and their catalytic mechanism and potential application
Authors: Zhang Qingdong , Cao Hai-Yan , Wei Lin , Lu Danrong , Du Min , Yuan Min , Shi Deling , Chen Xiangxue , Wang Peng , Chen Xiu-Lan , Chi Lianli , Zhang Yu-Zhong , Li fuchuan 
Published: August 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Physicians Views on Cardiovascular Disease Risk Prevention Services by Pharmacists and Potential for Collaboration
Authors: Almansour Hadi A. , Aloudah Nouf M. , Alhawassi Tariq M. , Chaar Betty , Krass Ines , Saini Bandana 
Published: August 2020

Abstract
Background: Cardiovascular diseases (CVD) are the leading cause of mortality worldwide. Early identification and management of modifiable CVD risk factors is highly effective in preventing disease onset and/or improving outcomes in CVD. Pharmacists as highly accessible primary health professionals can assume a role in screening and risk factor management in collaboration with physicians, however such prevention services are not established practice in Saudi pharmacies. Therefore, the aim was to explore physicians’ perceptions about the utility of a role in CVD risk screening and management for Saudi pharmacists. Methods: Qualitative semi-structured interviews were conducted, audio-recorded and transcribed verbatim in Arabic or English. All transcripts were thematically analysed after translation into English if required. Results: A total of 26 physicians recruited from public hospitals and primary healthcare centres were interviewed. Most were unaware of pharmacists’ potential to undertake a role in CVD risk prevention. Although there was broad support for the concept, they recommended physician-pharmacist collaborative models, extensive provider pharmacist training and strict oversight by the Saudi Ministry of Health/other official authorities to ensure service quality and sustainability, should implementation occur. Healthcare system reform was considered key to expanding private sector (i.e. community pharmacy) involvement in healthcare as was incentivising providers and ‘marketing’ for patient acceptance. Conclusion: Physicians were positive about setting up a collaborative community pharmacist-physician CVD risk screening and management service model with the help of an authorised body within the Saudi Arabian healthcare system.
Assessment of gingival inflammation indexes and matrix metalloproteinase (MMP-9) in patients with polycystic ovarian syndrome compared to control group
Authors: Maboudi Avideh , Shafizade Sara , Nasiriamiri Fatemeh , Qujeq Dourdi , Mohammadpour Reza Ali , Moaddabi Amirhosain , Amiri Mania , Bahnemiri Mehdi Gholami , Pasha Sadra Yosefnia 
Published: August 2020

Abstract
Background: There is emerging evidence to support the link between polycystic ovarian syndrome (PCOS) and periodontal disease, but the mechanisms associated with these two diseases have not been clearly elucidated but relate to different aspects of inflammation. This study aimed to determine the gingival inflammation indexes and matrix metalloproteinase-9 (MMP-9) in patients with polycystic ovarian syndrome compared to control group.Methods: This is a case-control study in which 87 women referred to the Babol Clinic Private Hospital were divided into three groups: 26 women with PCOS diagnosed on the basis of Rotterdam criteria with gingivitis, 26 women with PCOS without gingivitis and 26 healthy women who were matched for age and body mass index. After recording the underlying variables, fasting saliva samples were taken from all participants in the morning before any periodontal intervention. The samples, which were transferred to Babol Molecular Cell Research Center under cold-chain conditions to measure the serum levels of MMP-9 using ELISA kits. Periodontal status was evaluated for Gingival index (GI), bleeding on probing (BOP), plaque index (PI). Analysis of variance was used to compare the results for the normal quantitative variables and Chi-square test was used to compare the qualitative variables. Significance level was considered less than 0.05.Results: The highest mean periodontal disease indexes (GI, BOP, PI) were observed in women with PCOS with gingivitis and then in women with PCOS without gingivitis and then in healthy women in control group (p <0.05). The mean salivary MMP-9 levels were significantly higher in PCOS women (388/37 ± 75.05) compared to the control group (166/25 ± 35/43). Even in PCOS patients with healthy gingiva (233.00± 47.76) had higher levels of salivary MMP-9 than the control group and this difference was significant (p <0.05)Conclusions: There is a positive association between periodontal disease and PCOS and that salivary MMP-9 levels are higher in PCOS women with and without gingivitis than healthy women. However, it is recommended that multicenter study with larger sample sizes are to be conducted to establish a clearer association.
Robot-assisted sleeve gastrectomy in patients with obesity with a novel Chinese domestic MicroHand SⅡ surgical system
Authors: Li Weizheng , Kong Kang , Li Pengzhou , Wang Guohui , Cui Beibei , Zhu Liyong , Zhu Shaihong 
Published: August 2020

Abstract
Background

A new member has been added to the Chinese MicroHand surgical robot family, developed based on the successful application of control algorithms. As a benefit of using these specialized control algorithms, the motion mapping relation can be built up without the help of a built-in image system, resulting in a novel Chinese domestic surgical robot with two arms called MicroHand SⅡ, which is different from the former MicroHand S and da Vinci systems. The analysis technique of clinical practise using the Chinese domestic surgical robot to perform sleeve gastrectomy is not well presented. In this study, we investigate the performance of a novel MicroHand SⅡ robotic platform in patients with obesity.
Methods

From March 2018 to April 2019, a total of 7 patients affected by obesity were operated on with the robot-assisted technique using the MicroHand SⅡ surgical system. Data regarding demography, surgical procedure and the 3-month outcome postoperation were collected.
Results

There were 2 female and 5 male patients with a mean age of 34.0 ± 11.4 years. The mean operative time was 166.4 ± 16.1 minutes. None were converted to open surgery. All anthropometry indices improved significantly at 3 months postoperatively.
Conclusions

In conclusion, the Chinese domestic robot surgical system MicroHand SⅡ could be feasibly and safely applied in sleeve gastrectomy in patients with obesity. This robotic platform has presented its own obvious advantages.
TLR7 promotes smoke-induced lung damage through the activity of mast cell tryptase
Authors: Haw Tatt Jhong , Starkey Malcolm , Pavlidis Stelios , Tam Sheena , Nair Prema M. , Liu Gang , Gomez Henry M. , Hanish Irwan , Hsu Alan , Kim Richard , Fukui Ryutaro , Murakami Yusuke , Horvat Jay , Foster Paul , Oliver Brian , Wark Peter , Adcock Ian , Miyake Kensuke , Sin Don , Hansbro Philip 
Published: August 2020

Abstract
Toll-like receptor (TLR)7 is known for eliciting immunity against single-stranded RNA viruses. TLR7 was increased in both human and cigarette smoke (CS)-induced experimental chronic obstructive pulmonary disease (COPD). Severity of CS-induced emphysema and COPD was reduced in TLR7-deficient mice whilst inhalation of imiquimod (TLR7-agonist) induced emphysema in naïve mice. Imiquimod-induced emphysema was reduced in mice treated with mast cell stabilizer cromolyn or deficient in mast cell protease-6. Therapeutic treatment with anti-TLR7 monoclonal antibody suppressed CS-induced emphysema, experimental COPD and accumulation of pulmonary mast cells. We demonstrate an unexpected role for TLR7 in mediating emphysema and COPD through mast cell activity.
Circ-CCDC66 Upregulates REXO1 Expression to Aggravate Cervical Cancer Progression Via Restraining miR-452-5p
Authors: Zhang Yun , Li Xing , Zhang Jun , Mao Lin 
Published: August 2020

Abstract
BackgroundCervical cancer is one most common cancer types among females around the world. CircRNAs have been revealed to participate in multiple biological functions, and are involved in many diseases’ progression. In current study, we aimed to elucidate whether circ-CCDC66 participates cervical cancer progression. MethodsReal-time quantitative PCR (RT-qPCR) was conducted to measure the expression of circ-CCDC66, miR-452-5p, and REXO1 mRNA. Cell fractionation assay and RNA fluorescence in situ hybridization (FISH) were performed to locate circ-CCDC66 in cells. Cell proliferation ability was detected using cell account kit 8 (CCK-8). TRANSWELL assay was applied to evaluate cell migration or invasion ability. Bioinformatics analysis, biotinylated RNA pull-down, RNA immunoprecipitation, and dual-luciferase reporter assays were conducted to assess the association between miR-452 and circ-CCDC66 or REXO1. Western blot was applied to measure the protein expression of REXO1. Animal tumor model was used to assess the effect of circ-CCDC66 in vivo.ResultsCirc-CCDC66 was upregulated in cervical cancer tumor tissues, and correlated with tumor stage and tumor size. Downregulation of circ-CCDC66 inhibited cervical cancer cell proliferation, migration, and invasion. Circ-CCDC66 was an efficient molecular sponge for miR-452-5p, and negatively regulated miR-452-5p expression. MiR-452 directly targeted REXO1. The effects of circ-CCDC66 on cervical cancer cells were functioned through miR-452-5p/REXO1 axis. In animal experiment, downregulation of circ-CCDC66 was found to suppress tumor growth in vivo.ConclusionOur results demonstrated the effects of circ-CCDC66/miR-452-5p/REXO1 axis in cervical cancer progression, we might provide a novel therapeutic target for cervical cancer.
WITHDRAWN: Saudi Healthcare Facilities Risk Management and Infection Control Preparedness to Overcome Covid-19 Pandemic
Authors: Aleanizy Fadilah Sfouq , Alqahtani Fulwah Yahya 
Published: August 2020

Abstract
Background: The purpose of this study was to evaluate how well prepared Saudi healthcare facilities to overcome COVID-19 pandemic. Methods: A cross-sectional study was conducted using online-administered English questionnaire that distributed throughout Saudi commission for health specialities at Saudi hospital during the period of April-June 2020. The questionnaire represent a minimum level of knowledge and practices for all components of risk management and infection control and prevention program which should be known and practiced by all healthcare workers. The study population included all healthcare workers in Saudi hospitals designated to deal with COVID-19 cases. Results: The study revealed that the overall knowledge of risk management plan and infection prevention control was found to be comparatively excellent (80.4%), and there were no discrepancies among the participants according to gender, education, occupation, however, some differences and variation according to age group and the years of experience. Most responses were from hospital located in central province (72.7%), governmental hospital (88%), and mostly provide family services (68%). The majority of hospitals have isolation units (88.2%) either under the authority of Ministry of health or not. Also there were enough training for medical staff which is considered as a base for good practice of risk management and infection control and prevention procedures, policies and guidelines. Conclusion: The study concluded that outstanding knowledge of all healthcare workers at Saudi hospitals regarding risk management plan and pandemic sub-plan of infection control and prevention policies procedures and principles, regardless the variations among the staff, however these base knowledge have helped Saudi Arabia health authority to successfully overcome COVID-19.
The voltage-gated proton channel Hv1 contributes to neuronal injury and motor deficits in a mouse model of spinal cord injury
Authors: Murugan Madhuvika , Zheng Jiaying , Wu Gongxiong , Mogilevsky Rochelle , Zheng Xin , Hu Peiwen , Wu Junfang , Wu Long-Jun 
Published: August 2020

Abstract
Traumatic injury to the spinal cord initiates a series of pathological cellular processes that exacerbate tissue damage at and beyond the original site of injury. This secondary damage includes oxidative stress and inflammatory cascades that can lead to further neuronal loss and motor deficits. Microglial activation is an essential component of these secondary signaling cascades. The voltage-gated proton channel, Hv1, functionally expressed in microglia has been implicated in microglia polarization and oxidative stress in ischemic stroke. Here, we investigate whether Hv1 mediates microglial/macrophage activation and aggravates secondary damage following spinal cord injury (SCI). Following contusion SCI, wild-type (WT) mice showed significant tissue damage, white matter damage and impaired motor recovery. However, mice lacking Hv1 (Hv1-/-) showed significant white matter sparing and improved motor recovery. The improved motor recovery in Hv1-/- mice was associated with decreased interleukin-1β, reactive oxygen/ nitrogen species production and reduced neuronal loss. Further, deficiency of Hv1 directly influenced microglia activation as noted by decrease in microglia numbers, soma size and reduced outward rectifier K+ current density in Hv1-/- mice compared to WT mice at 7d following SCI. Our results therefore implicate that Hv1 may be a promising potential therapeutic target to alleviate secondary damage following SCI caused by microglia/macrophage activation.
The Complete Chloroplast Genome of Tamarix Ramosissima and Comparative Analysis of Tamaricaceae Species&nbsp;
Authors: Wang Le , Wang Li , Guo Zhihong 
Published: August 2020

Abstract
Background: Tamarix ramosissima is a deciduous shrub resided in arid and semi-arid regions. Although of ecological and medicinal values, some Tamarix species are considered invasive as they have dominated the riparian zones of dryland in some parts of the world. Chloroplast (cp) DNA is highly conserved in structure and gene arrangement, making cp genomic data valuable resources for species delimitation and phylogenetics. The cp genome of T. ramosissima was de novo assembled with the aim of providing reference and data resource for further cp-derived marker development and species delimitation of Tamarix.Results: Here, the complete chloroplast (CP) genome of T. ramosissima was sequenced and analyzed, showing a size of 156150 bp and a GC content of 36.5%. The plastome displayed a typical quadripartite structure, consisting of a pair of inverted repeat (IR) regions of 26554 bp, separated by a large single copy (LSC) region of 84795 bp, and a small single copy (SSC) region of 18247 bp. The cp genome encoded 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. A total of 32 repeat sequences and 64 simple sequence repeats (SSR) were identified in the plastome, and an obvious A/T bias was observed in the majority of the SSRs detected. By comparing the T. ramosissima cp genome with those of four other Tamaricaceae species, a number of divergence hotspots were identified among these plastomes. Together with the SSRs and long repeats identified, these divergence hotspots could be developed as potential molecular markers facilitating species discrimination and evolutionary studies. Using plastome sequences, we re-investigated the phylogenetic relationship among 19 species, and T. ramosissima was found to be a sister of Tamarix chinensis.Conclusions: Taken together, our study provides valuable genomic resources to deepen the understanding of the plant photosynthetic mechanism and phylogenomics.