bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiovascular risk prediction in diabetes – changes in the prognostic values of modern cardiovascular biomarkers in relation to duration of diabetes mellitus
Authors: Resl Michael , Vila G , Heinzl M , Luger A , Neuhold S , Prager R. , Wurm R , Hülsmann M , Clodi M 
Published: October 2020

Abstract
Background

Based on the complex pathophysiology of type 2 diabetes and atherosclerosis we hypothesized a dynamic change in prognostic value of cardiovascular biomarkers over time.
Methods

In this prospective study 746 patients with type 2 diabetes mellitus, being followed up for 60 months were analysed. The primary endpoint was defined as unplanned hospitalization for cardiovascular disease or death. Beside others, especially the prognostic performance of the biomarkers of interest (GDF-15, NT-proBNP, hs-TnT) was evaluated in relation to quartiles of diabetesduration.
Results

In patients having a diabetes duration below 7 years lnGDF-15 (HR 2.84; p < 0.01) and lnhs-TnT (HR 2.96; p < 0.01) were significant predictors of the primary endpoint. LnAge (HR 40.11; p < 0.01) and lnNT-proBNP (HR 1.61; p < 0.01) were significant predictors in patients with a diabetes duration between 7 and 12 years. In the third quartile (diabetes duration 12–22 years) lnAge (HR 13.09; p = 0.041), urinary albumin to creatinine ratio (HR 2.74; p = 0.001) and lnNT-proBNP (HR 1.81, p < 0.001) predicted the endpoint. In patients with a diabetes duration above 22 years, lnAge (HR 50.79; p = 0.002) and lnNT-proBNP (HR 2.1; p < 0.01) were the only significant predictors of the endpoint.
Conclusion

Prognostic power of cardiovascular biomarkers changes dynamically in relation to duration of type 2 diabetes mellitus. In patients with shorter duration of the disease markers of subclinical cardiovascular dysfunction and inflammation perform better than markers of systemic advanced organ dysfunction and cardiovascular disease.
The non-relativistic and relativistic quantum theory with temperature
Authors: Xiang-Yao Wu , Wu Ben-Shan , Liu Han 
Published: October 2020

Abstract
In this paper, we have proposed the principle of quantum
thermodynamics, including energy principle and microcosmic entropy
principle, and given the quantum thermodynamics of
non-relativistic and relativistic quantum theory, i.e., the
temperature-dependent schrodinger equation, Dirac equation and
photon equation. We given the solution for wave function and
energy level with temperature. Taking the hydrogen atom as an
example, we given the temperature correction to hydrogen atom
energy level and wave function.
Clean Technology Approach For Ciprofloxacin Sequestration Using Modified Banana Stalk
Authors: OREOFE Toyin Adedayo , OGUNLEYE Oladipupo Olaosebikan , Bello Olugbenga Solomon 
Published: October 2020

Abstract
The adsorption mechanisms and capacity of adsorbent derived from banana stalks in removing ciprofloxacin (CIP) from aqueous solutions were investigated in this study. The Banana Stalk Activated Carbon (BSAC) was prepared through acid activation and were characterised via SEM, FTIR and pHpzc. The SEM micrograph of BSAC revealed prominent well-developed pores which aid the adsorption process. The FTIR spetra shows the presence of various functional groups for efficient CIP adsorption. The effects of temperature (30 – 70 oC), adsorbent dosage (0.01 – 0.05 g/L), pH (2 - 10),initial adsorbate concentration (1 - 5 mg/L) and contact time (10 - 360 min) were evaluated using batch operations. Optimum CIP adsorption was obtained at pH 6. The maximum monolayer adsorptive capacity of the acid-treated banana stalk was 222.22 mg/g at 30 oC. Langmuir isotherm was the most appropriate isotherm model while pseudo second order kinetics best describe the kinetic process. Its thermodynamic studies indicated endothermic and spontaneous process. The cost implication of BSAC preparation (12.35 USD/kg) indicates that it is about 21 times cheaper than Commercial activated carbon (270.8 USD/kg) while the desorption studies reflects the reusability efficiency of the spent adsorbent. BSAC was found to be effective, efficient and economically viable for ciprofloxacin removal from aqueous solution.
The effect of different drying methods on non-volatile and volatile components and immunomodulatory activity of Osmamthus fragrans flowers
Authors: Chang Yuexing , Lin Junjie , Pan Siqing , Jing Yanlin , Guo Ailing , Deng Yun 
Published: October 2020

Abstract
Background: Osmamthus fragrans (O. fragrans) has high ornamental, edible and medicinal value in China. The components of its flowers have been gradually revealed, but the active ingredients with immunoregulatory activity have been unknown. Also, it needs to be studied that which groups and drying methods can make O. fragrans fowers produce more immunomodulatory activity. This study aimed to investigate the effects of drying methods on non-volatile and volatile components of O. fragrans flowers from three groups, and to further explore if their groups and drying methods had an ovious effect on their immunoregulatory activity.Methods: O. fragrans flowers from three groups such as “Aurantiacus”, “Latifolius” and “Thunbergii” were dried with shade drying, sun drying, quick-lime drying, oven drying and microwave drying method, respectively. The non-volatile components such as salidroside, verbascoside, oleanolic acid and ursolic acid and volatile components such as linalool, linalool oxide, geraniol, α-ionone and β-ionone of O. fragrans flowers above were determined by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. The immunomodulatory activities of chemical components above were investigated by the neutral red uptake assay.Results: There are more non-volatile components and less volatile components in O. fragrans flowers from Aurantiacus group and Latifolius group than Thunbergii group. Microwave drying and oven drying at high temperature were beneficial for the preservation of the bioactive non-volatile components for killing enzyme and protecting glycoside. Other drying methods such as shade drying, sun drying, quick-lime drying and oven drying at low temperature were beneficial to their preservation of the fragrant volatile components. Salidroside, verbascoside, linalool, and linalool oxide had the better immunoregulatory activity than other ingredients. In addition, non-volatile components played a more important role in the contribution to the immunoregulatory activity than the volatile components as the former was almost 1000 times as much as the latter. Conclusions: O. fragrans flowers from Aurantiacus group with microwave drying (high fire) method had the best immunoregulatory activity. The research could provide some evidence in choosing drying method of O. fragrans flowers as food or medicine.
Two-way covert quantum communication in the microwave regime
Authors: Candia Roberto Di , Yiğitler Hüseyin , Paraoanu Gheorghe , Jäntti Riku 
Published: October 2020

Abstract
Quantum communication addresses the problem of exchanging information across macroscopic distances by employing encryption techniques based on quantum mechanical laws. Here, we advance a new paradigm for secure quantum communication by combining backscattering concepts with covert communication in the microwave regime. Our protocol allows communication between Alice, who uses only discrete phase modulations, and Bob, who has access to cryogenic microwave technology. Using notions of quantum channel discrimination and quantum metrology, we find the ultimate bounds for the receiver performance, proving that quantum correlations can enhance the signal-to-noise ratio by up to 6 dB. We show that security can be reached by covering the carrier signal through the presence of the thermal noise in the environment. We complement our information-theoretic results with a feasible experimental proposal in a circuit QED platform. This work makes a decisive step toward implementing secure quantum communication concepts in
the previously uncharted 1 − 10 GHz frequency range, in the relevant scenario when the available power is severely constrained.
Assessment on the lung injury of mice posed by airborne PM2.5 collected from developing area in China and associated molecular mechanisms: from histopathology to integrated transcriptome analysis
Authors: Dai Shuiping , Wang Zhenglu , Yang Ying , Guo Tingting , li weimin 
Published: October 2020

Abstract
Background & aimsSome epidemiological investigations have revealed airborne fine particulate matter (PM2.5) could induce adverse effects on respiratory system of human. However, the experimental evidences of the harmful effect of PM2.5 from mid-scale city in China and associated molecular mechanisms was still scarce. In this study, we aimed to evaluate the adverse effect on the lung system of PM2.5 collected from mid-city of China and elucidate the underlying molecular mechanism through mRNA-seq and microRNA-seq integrated analysis.MethodsWe exposed male Balb/C mice to PM2.5 collected from Baoji city, China for 8 weeks (298.52 ± 25.86 μg/m3) using a whole-body exposure system. Micro-CT and histopathological analyses were performed to determine the morphology and histopathology changes of lung tissues induced by PM2.5. Transcriptome (both mRNA and microRNA) sequencing and the immunohistochemistry assay were performed to reveal the associated underlying mechanisms. The contents of PAHs absorbed in the PM2.5, as well as the pearson correlation index between it and the target genes and microRNA were examined.ResultsObvious lung injury including pulmonary dysfunction, immunological responses, and fibrosis were observed from the PM2.5 exposure group. The content of leucocyte of lung tissue was significantly increased. PM2.5 mainly induced immune pathways including B cell receptor signaling and cell adhesion molecules (CAMs). The expression levels of the associated genes and microRNA were verified using RT-qPCR. The protein expression of CD19, CD81, and PIK3CD involved into B cell receptor signaling significantly increased in exposure group. The concentrations of benzo(a)pyrene (BaP) showed a marked correlationship with the genes and microRNA. ConclusionsPM2.5 from the mid-scale city of China caused adverse effects on lung system of mice, which was much stronger than the mega city, indicating the health risk of the air pollution in the developing area should be paid more attention. The toxicity of PM2.5 may associated with immune system response. This work for the first time provided a new sight into the molecular mechanism of PM2.5 toxicity on lung system using transcriptomics integrated analysis and help further to develop effective measures to prevent air pollution associated lung disease, especially in the developing area in China.
The Biological and Clinical Significance of Glutaminase in Luminal Breast Cancer
Authors: Masisi Brendah , El-Ansari Rokaya , Alfarsi Lutfi , Craze Madeleine , Jewa Natasha , Oldfield Andrew , Cheung Hayley , Toss Michael , Rakha Emad , Green Andrew 
Published: October 2020

Abstract

Background: Glutamine metabolism has a key role in the regulation of uncontrolled tumour growth by modulating bioenergetics, redox homeostasis and serving as a precursor for biomass synthesis. Glutaminase is a key enzyme involved in glutaminolysis, a process which plays a crucial role in carcinogenesis and progression. This study aimed to evaluate the expression and prognostic significance of glutaminase in luminal breast cancer (BC). Methods: The glutaminase protein isoforms (GLS and GLS2) were assessed at the genomic and transcriptomic levels, using METABRIC (n=1398) and GENE MINER datasets (n=4,712), and protein level using immunohistochemistry in large well characterised cohorts of luminal Oestrogen Receptor (ER)-positive and HER2-negative BC patients, including ductal carcinoma in situ (DCIS) (n=206) and invasive BC (IBC; n=717) cohorts. GLS and GLS2 expression was associated with clinicopathological features, patient outcome and other glutamine-metabolism related genes. Results: In DCIS, GLS expression was an independent risk factor for shorter local recurrence-free interval (p<0.0008). In IBC high GLS and GLS2 mRNA and protein expression significantly correlated with solute carriers with high glutamine affinity, SLC3A2 (p≤0.01) SLC7A8 (p≤0.01) and SLC7A5 (p<0.001), and glutamine related enzymes; GLUD1 (p<0.001) and ALDH18A1 (p<0.001). GLS and GLS2 gene copy number gains were associated with poor patient outcome (p=0.028; p=0.010 respectively). High GLS2 protein was predictive of a longer disease-free survival (p=0.006). Conclusion: GLS appears to play a role in the early non-invasive stage of BC and it could be used as a potential biomarker to predict DCIS progression to invasive disease. In IBC, both GLS and GLS2 play a key role in the biological function of luminal tumours. Further functional assessments are needed to explore the specific role played by each isoform in BC.
Soluble B7-H4 and its association with clinical characteristics and prognosis in patients with early breast cancer
Authors: Mach Pawel , Hoffmann Oliver , Kasimir-Bauer Sabine , Kimmig Rainer , Schmidt Boerge , Schapeler Julia , Bittner Ann-Kathrin 
Published: October 2020

Abstract
Background: Immunotherapy is a promising area for treatment of breast cancer (BC) that has transformed patient care. Immune checkpoint inhibitors are only effective in a subset of patients, and the identification of biomarkers that predict response to therapy is crucial to increase the rates of responders. B7-H4 is a potentially novel target for cancer therapy.Methods: We examined the association of sB7-H4 with clinical characteristics and prognosis in patients with early BC. Using ELISA, we analyzed sB7-H4 serum concentrations in a total of 572 early BC patients before the onset of therapy, 109 patients (cohort 1) in the neo-adjuvant setting and 463 patients in the adjuvant setting (cohort 2). In cohort 1, measurements were also performed after neo-adjuvant therapy (NACT).Results: In cohort 1, sB7-H4 blood serum concentration was delectable in 27/109 (26%) patients before and in 50/109 (48%) patients after NACT. In cohort 2, the detection rate was only 4% (18/461 patients). In cohort 1, no significant differences between patients, even when stratifying for particular intrinsic subtypes, before and after NACT were observed. No significant chances in sB7-H4 blood serum concentration levels before and after NACT were associated with clinical parameters, prognosis and the risk of recurrence. The median blood serum concentration levels in cohort 2 were significantly higher than in cohort 1 after NACT (p=0.04) but not before NACT. Conclusions: sB7-H4 concentration levels in serum of non-metastatic BC patients are neither associated with prognosis nor with clinical characteristics in the adjuvant and neo-adjuvant setting.
Global Research Status and Development of COVID-19 in 2020: A Bibliometric Analysis
Authors: Pan Xiaobin , Lin Wei , Xie Jinbao , Lin Xingsheng , Zhang Yingfeng , Zhang Lihui , Wu Chao , Ye Mei , Shi Songchang , Shi Songjing , Chen Gang 
Published: October 2020

Abstract
Background: The coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is currently widespread in the world. This study aimed to access the characteristics of the publications involving COVID-19 by using a bibliometric analysis.Methods: COVID-19 publications published between 1 January 2020 and 31 July 2020 was searched from the Web of Science database on 1 August 2020.The database retrieval was done on the same day. Analysis parameters mainly include publication month, research institutions, authors, journals, countries and cooperation networks among them.Results: A total of 14186 COVID-19 associated articles were retrieved from the Web of Science database, and the quantity of articles increased rapidly month by month. The authors of the top ten manuscripts per number of citations and the most productive institution were both from China. The total publication number of China was as high as 3,029，second only to the United States. Moreover, China ranks first in the number of total citations of articles and the average article citations. The United States has the highest number of total publications and ranks second only to China in terms of the influence of individual articles. Authors, institutions and Countries established a network of close cooperation for research on COVID-19.Conclusion: There was a growing number of articles on COVID-19 around the world, China and the United States are the two most influential countries.
Patterns and Related Influencing Factors of Multiple Chronic Diseases Among the Elderly Based on Data Mining in Shanxi, China
Authors: Zhang Chichen , Xiao Shujuan , Shi Lei , Zheng Xiao , Dong Fang , Xue Yaqing , Zou Jiayu , Zhang Jiachi 
Published: October 2020

Abstract
Background: The presence of multiple chronic diseases has become a public health issue worldwide. The study aimed to explore the patterns and related influencing factors of multiple chronic diseases among the elderly so as to provide a scientific reference for the health management strategy, which is dedicated to the prevention and intervention for multiple chronic diseases. Methods: A cross-sectional study, which used a multi-stage random sampling way, was conducted among 3266 elderly aged 60 years and above in Shanxi. A chi square test was used to compare the sociodemographic factors. The data mining methods, including association rule and decision tree, were performed to explore the patterns and related influencing factors of multiple chronic diseases in the elderly.Results: The prevalence of multiple chronic diseases was 30.31%. According to the results of the association rules, there were 10 association patterns screened out. The first five multiple chronic disease patterns were atherosclerosis and hypertension, coronary heart disease and hypertension, diabetes and hypertension, stroke and hypertension, eye disease and hypertension. The decision tree model selected 5 related influencing factors of multiple chronic diseases, including age, smoking, family genetic history, fresh fruit intake frequency and education level, simultaneously generating 9 influence rules.Conclusions: Multiple chronic diseases were prevalent among the elderly, which patterns mainly consisted of two or three chronic diseases. Regarding the unmodifiable related influencing factors, including an older age, a family genetic history, and low education level, relevant departments should help the elderly to establish prevention knowledge and raise awareness of strongly related chronic diseases, so as to reduce the incidence and adverse consequences.
The Diagnostic Properties of the Translated Chinese Whooley Questionnaire as A Case-Finding Instrument for Depression Among Chinese Women During and After Pregnancy
Authors: Lok Kris Yuet-Wan , Chow Charlotte , Tan Shirley W , Smith Robert , Lin Jessie , Kong Choi Wah , Fong Daniel YT 
Published: October 2020

Abstract
Introduction: Rising prevalence and undetected perinatal depression has been described in many countries and report that treating those who are already symptomatic, more effort should be targeted towards screening strategies to identify perinatal depression at the early stage. The Whooley questions is the recommended case finding strategy to aid the identification of perinatal depression. An official Chinese version has not been validated. The aim of this study was to evaluate the diagnostic accuracy and stability of the translated Whooley questionnaire against the gold standard measurement during pregnancy (antenatal) and early after pregnancy (postnatal).Materials and method: This observational study recruited 131 pregnant women from antenatal clinics in a hospital setting from September 2019 till May 2020 in Hong Kong. We translated the Whooley questionnaire in Chinese and evaluated self-reported responses against an interviewer assessed diagnostic standard (DSM-IV criteria) among 107 women receiving antenatal care at 26-28 weeks gestation. We calculated sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio, negative likelihood ratio and diagnostic odds ratio, with DSM-IV diagnosis taken as the gold standard.Results: Antenatally, the Chinese Whooley questions had a sensitivity of 79% (95% CI 54.4-93.9), a specificity of 97% (95% CI 90.4-99.3), a positive likelihood ratio of 23.2 (95% CI 7.4-72.1) and a negative likelihood ratio of 0.2 (95% CI 0.1-0.5) in identifying perinatal depression. Conclusion: Prevalence of depression in pregnancy have increased and screening early remains a significant tool in Western countries. The translated Chinese Whooley questionnaire appears to have acceptable diagnostic accuracy and can be implemented into health services among Chinese population as only requiring two yes/no questions.
SNPs in Apolipoproteins Contribute to Sex-Dependent Differences in Blood Lipids Before and After A Dietary Challenge in Healthy U.S. Adults
Authors: Wang Yining E. , Kirschke Catherine P. , Woodhouse Leslie R. , Bonnel Ellen L. , Stephensen Charles B. , Bennett Brian J , Newman John W. , Keim Nancy L. , Huang Liping 
Published: October 2020

Abstract
Background The effect of genetic polymorphisms on fasting blood lipid levels have been widely studied but the effects of these within the context of a meal challenge remain less characterized. The current study aimed to investigate the association of SNPs in lipoprotein-related genes with blood lipid levels in healthy adults in the U.S. Methods Men and women (n = 393) between 18-66 years of age with BMIs ranging from 18.5-45 kg/m 2 completed the cross-sectional Nutritional Phenotyping Study. Among them, 349 subjects (men = 167, 48%; women = 182, 52%) gave consent for genotyping. SNPs in APOA5 , APOB , APOC3 , APOE , and LDLR were assessed. Results Reduced blood HDL-cholesterol levels were associated with the C allele of the APOA5 SNP (rs3135506) in women and the C allele of the APOE SNP (rs429358) in men in both fasting and postprandial states. The C allele of the APOE SNP was also correlated with increased LDL-C levels. The APOC3 rs2854116 TT genotype was associated with elevated total cholesterol in both sexes. Nevertheless, these SNPs had little impact on the postprandial triglyceride responses to the high-fat challenge meal. Additionally, no significant effects of SNPs in APOB (rs1042034) and LDLR (rs2228671) on triglycerides, cholesterol, or non-esterified free fatty acids levels were found. Conclusions Cholesterol levels are strongly correlated with genotypes of the tested SNPs in APOA5 and APOE in a sex-dependent manner during fasting and postprandial states of healthy adults. Trial registration: ClinicalTrials.gov, NCT02367287. Registered February 20, 2015, https://clinicaltrials.gov/ct2/show/NCT02367287.