bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison of fecal sampling and direct feeding observations for quantifying the diet of a frugivorous primate
Authors: Matthews Jaya K , Ridley Amanda , Kaplin Beth A , Grueter Cyril C 
Published: November 2019

AbstractBoth observational and indirect evidence are widely used to determine the diets of wild animals. Direct observations are often assumed to provide the most comprehensive reflection of diet, but many wild animals are logistically challenging to observe. Despite the regular use of observational and indirect methods for inferring diet in wild animals, they have rarely been compared in detail for the same study population. Over 12 months this study assessed the congruence of methods in estimating the diet of a montane community of eastern chimpanzees Pan troglodytes schweinfurthii in Nyungwe National Park, Rwanda using observational scan samples and macroscopic fecal inspection. The assessment of the number of food species consumed each month was comparable between methods, but the estimation of the composition of items in the diet differed significantly. Most notably, the fecal samples significantly underestimated the consumption of flowers, and certain fruit species, which based on direct behavioral observations were seasonally consumed at very high rates. Conversely, direct observations underestimated the consumption of leaves and pith in comparison to results present in the fecal samples. These results suggest that combining methods where possible is most useful for accurate monitoring of dietary trends, particularly for species that experience significant seasonal shifts in their diet.
The Genome of the Blind Soil-Dwelling and Ancestrally Wingless Dipluran Campodea augens: A Key Reference Hexapod for Studying the Emergence of Insect Innovations
Authors: Manni Mosè , Simao Felipe A , Robertson Hugh M , Gabaglio Marco A , Waterhouse Robert M , Misof Bernhard , Niehuis Oliver , Szucsich Nikolaus U , Zdobnov Evgeny M 
Published: November 2019

AbstractThe dipluran two-pronged bristletail Campodea augens is a blind ancestrally wingless hexapod with the remarkable capacity to regenerate lost body appendages such as its long antennae. As sister group to Insecta (sensu stricto), Diplura are key to understanding the early evolution of hexapods and the origin and evolution of insects. Here we report the 1.2-Gb draft genome of C. augens and results from comparative genomic analyses with other arthropods. In C. augens, we uncovered the largest chemosensory gene repertoire of ionotropic receptors in the animal kingdom, a massive expansion that might compensate for the loss of vision. We found a paucity of photoreceptor genes mirroring at the genomic level the secondary loss of an ancestral external photoreceptor organ. Expansions of detoxification and carbohydrate metabolism gene families might reflect adaptations for foraging behavior, and duplicated apoptotic genes might underlie its high regenerative potential. The C. augens genome represents one of the key references for studying the emergence of genomic innovations in insects, the most diverse animal group, and opens up novel opportunities to study the under-explored biology of diplurans.
Age and Gender Stereotypes Reflected in Google’s “Autocomplete” Function: The Portrayal and Possible Spread of Societal Stereotypes
Authors: Roy Senjooti , Ayalon Liat 
Published: November 2019

Abstract

Background and Objectives
Google’s autocomplete function provides “predictors” to enable quick completion of intended search terms. The predictors reflect the search trends of a population; they capture societal beliefs and perceptions about a variety of subjects. This study explores the predictors provided by Google United States when searching for information about older men and women.

Research Design and Methods
The Google search engine of the United States was used to record predictors in response to 720 search terms. Inductive content analysis method was used to categorize and interpret the results.

Results
There was prevalence of age and gender stereotypes in the predictors offered by Google. Queries about older men were primarily associated with romance and sexuality whereas those for older women centered on changes in the body as well as sexual and reproductive health.

Discussion and Implications
The appearance of such predictors in response to a potential search query may lead to the spread of harmful stereotypes about older adults.
Naturally occurring NOTCH3 exon skipping attenuates NOTCH3 protein aggregation and disease severity in CADASIL patients
Authors: Gravesteijn Gido , Dauwerse Johannes G , Overzier Maurice , Brouwer Gwendolyn , Hegeman Ingrid , Mulder Aat A , Baas Frank , Kruit Mark C , Terwindt Gisela M , van Duinen Sjoerd G , Jost Carolina R , Aartsma-Rus Annemieke , Lesnik Oberstein Saskia A J , Rutten Julie W 
Published: November 2019

Abstract
CADASIL is a vascular protein aggregation disorder caused by cysteine-altering NOTCH3 variants, leading to mid-adult-onset stroke and dementia. Here, we report individuals with a cysteine-altering NOTCH3 variant that induces exon 9 skipping, mimicking therapeutic NOTCH3 cysteine correction. The index came to our attention after a coincidental finding on a commercial screening MRI, revealing white matter hyperintensities. A heterozygous NOTCH3 c.1492G&gt;T, p.Gly498Cys variant, was identified using a gene panel, which was also present in four first- and second-degree relatives. Although some degree of white matter hyperintensities was present on MRI in all family members with the NOTCH3 variant, the CADASIL phenotype was mild, as none had lacunes on MRI and there was no disability or cognitive impairment above the age of 60 years. RT-PCR and Sanger sequencing analysis on patient fibroblast RNA revealed that exon 9 was absent from the majority of NOTCH3 transcripts of the mutant allele, effectively excluding the mutation. NOTCH3 aggregation was assessed in skin biopsies using electron microscopy and immunohistochemistry and did not show granular osmiophilic material and only very mild NOTCH3 staining. For purposes of therapeutic translatability, we show that, in cell models, exon 9 exclusion can be obtained using antisense-mediated exon skipping and CRISPR/Cas9-mediated genome editing. In conclusion, this study provides the first in-human evidence that cysteine corrective NOTCH3 exon skipping is associated with less NOTCH3 aggregation and an attenuated phenotype, justifying further therapeutic development of NOTCH3 cysteine correction for CADASIL.
Sources of Stability in Social and Economic Ideological Orientations: Cohort, Context, and Construct Effects
Authors: Ksiazkiewicz Aleksander , Klemmensen Robert , Dawes Christopher T , Christensen Kaare , McGue Matt , Krueger Robert F , Nørgaard Asbjørn Sonne 
Published: November 2019

AbstractExisting research shows that ideological orientations are stable after young adulthood. Extending research on the sources of ideological stability, we examine social and economic ideology over a 3- to 4-year period in two twin panels (one Danish and one American). We find evidence for the importance of genetic influences and individual life experiences on the stability of social ideology in both contexts; shared environmental factors play an important role in the younger, Danish sample only. For economic ideology, genetic factors contribute to stability in the American sample only. Our findings show that the role of genetic and environmental factors in the stability of ideological orientations varies by type of ideology, national context, and, possibly, age cohort.
A Malaria Transmission Model Predicts Holoendemic, Hyperendemic, and Hypoendemic Transmission Patterns Under Varied Seasonal Vector Dynamics
Authors: Ratti Vardayani , Wallace Dorothy I 
Published: November 2019

Abstract
A model is developed of malaria (Plasmodium falciparum) transmission in vector (Anopheles gambiae) and human populations that include the capacity for both clinical and parasite suppressing immunity. This model is coupled with a population model for Anopheles gambiae that varies seasonal with temperature and larval habitat availability. At steady state, the model clearly distinguishes uns hypoendemic transmission patterns from stable hyperendemic and holoendemic patterns of transmission. The model further distinguishes hyperendemic from holoendemic disease based on seasonality of infection. For hyperendemic and holoendemic transmission, the model produces the relationship between entomological inoculation rate and disease prevalence observed in the field. It further produces expected rates of immunity and prevalence across all three endemic patterns. The model does not produce mesoendemic transmission patterns at steady state for any parameter choices, leading to the conclusion that mesoendemic patterns occur during transient states or as a result of factors not included in this study. The model shows that coupling the effect of varying larval habitat availability with the effects of clinical and parasite-suppressing immunity is enough to produce known patterns of malaria transmission.
Double-blind reviewing and gender biases at EvoLang conferences: An update
Authors: Cuskley Christine , Roberts Seán G , Politzer-Ahles Stephen , Verhoef Tessa 
Published: November 2019

AbstractA previous study of reviewing at the Evolution of Language conferences found effects that suggested that gender bias against female authors was alleviated under double-blind review at EvoLang 11. We update this analysis in two specific ways. First, we add data from the most recent EvoLang 12 conference, providing a comprehensive picture of the conference over five iterations. Like EvoLang 11, EvoLang 12 used double-blind review, but EvoLang 12 showed no significant difference in review scores between genders. We discuss potential explanations for why there was a strong effect in EvoLang 11, which is largely absent in EvoLang 12. These include testing whether readability differs between genders, though we find no evidence to support this. Although gender differences seem to have declined for EvoLang 12, we suggest that double-blind review provides a more equitable evaluation process.
Time-dependent behaviour of quasar proximity zones at z ∼ 6
Authors: Davies Frederick B , Hennawi Joseph F , Eilers Anna-Christina 
Published: November 2019

ABSTRACT
Since the discovery of z ∼ 6 quasars two decades ago, studies of their Ly α-transparent proximity zones have largely focused on their utility as a probe of cosmic reionization. But even when in a highly ionized intergalactic medium, these zones provide a rich laboratory for determining the time-scales that govern quasar activity and the concomitant growth of their supermassive black holes. In this work, we use a suite of 1D radiative transfer simulations of quasar proximity zones to explore their time-dependent behaviour for activity time-scales from ∼103 to 108 yr. The sizes of the simulated proximity zones, as quantified by the distance at which the smoothed Ly α transmission drops below 10 per cent (denoted Rp), are in excellent agreement with observations, with the exception of a handful of particularly small zones that have been attributed to extremely short ≲104 lifetimes. We develop a physically motivated semi-analytic model of proximity zones which captures the bulk of their equilibrium and non-equilibrium behaviour, and use this model to investigate how quasar variability on ≲105 yr time-scales is imprinted on the distribution of observed proximity zone sizes. We show that large variations in the ionizing luminosity of quasars on time-scales of ≲104 yr are disfavoured based on the good agreement between the observed distribution of Rp and our model prediction based on ‘lightbulb’ (i.e. steady constant emission) light curves.
Genetic Landscapes Reveal How Human Genetic Diversity Aligns with Geography
Authors: Peter Benjamin M , Petkova Desislava , Novembre John 
Published: November 2019

Abstract
Geographic patterns in human genetic diversity carry footprints of population history and provide insights for genetic medicine and its application across human populations. Summarizing and visually representing these patterns of diversity has been a persistent goal for human geneticists, and has revealed that genetic differentiation is frequently correlated with geographic distance. However, most analytical methods to represent population structure do not incorporate geography directly, and it must be considered post hoc alongside a visual summary of the genetic structure. Here, we estimate “effective migration” surfaces to visualize how human genetic diversity is geographically structured. The results reveal local patterns of differentiation in detail and emphasize that while genetic similarity generally decays with geographic distance, the relationship is often subtly distorted. Overall, the visualizations provide a new perspective on genetics and geography in humans and insight to the geographic distribution of human genetic variation.
Mutations Beget More Mutations—Rapid Evolution of Mutation Rate in Response to the Risk of Runaway Accumulation
Authors: Ruan Yongsen , Wang Haiyu , Chen Bingjie , Wen Haijun , Wu Chung-I 
Published: November 2019

AbstractThe rapidity with which the mutation rate evolves could greatly impact evolutionary patterns. Nevertheless, most studies simply assume a constant rate in the time scale of interest (Kimura 1983; Drake 1991; Kumar 2005; Li 2007; Lynch 2010). In contrast, recent studies of somatic mutations suggest that the mutation rate may vary by several orders of magnitude within a lifetime (Kandoth et al. 2013; Lawrence et al. 2013). To resolve the discrepancy, we now propose a runaway model, applicable to both the germline and soma, whereby mutator mutations form a positive-feedback loop. In this loop, any mutator mutation would increase the rate of acquiring the next mutator, thus triggering a runaway escalation in mutation rate. The process can be initiated more readily if there are many weak mutators than a few strong ones. Interestingly, even a small increase in the mutation rate at birth could trigger the runaway process, resulting in unfit progeny. In slowly reproducing species, the need to minimize the risk of this uncontrolled accumulation would thus favor setting the mutation rate low. In comparison, species that starts and ends reproduction sooner do not face the risk and may set the baseline mutation rate higher. The mutation rate would evolve in response to the risk of runaway mutation, in particular, when the generation time changes. A rapidly evolving mutation rate may shed new lights on many evolutionary phenomena (Elango et al. 2006; Thomas et al. 2010, 2018; Langergraber et al. 2012; Besenbacher et al. 2019).
Chronic kidney disease progression and mortality risk profiles in Germany: results from the Chronic Kidney Disease Outcomes and Practice Patterns Study
Authors: Reichel Helmut , Zee Jarcy , Tu Charlotte , Young Eric , Pisoni Ronald L , Stengel Bénédicte , Duttlinger Johannes , Lonnemann Gerhard , Robinson Bruce M , Pecoits-Filho Roberto , Fliser Danilo 
Published: November 2019

Abstract

Background
Chronic kidney disease (CKD) progression among German patients in a representative setting has not been described previously. The Verband Deutsche Nierenzentren and Chronic Kidney Disease Outcomes and Practice Patterns Study established a longitudinal observational cohort among German CKD patients to research variations in patient care and outcomes in real-world nephrology practices.

Methods
A cohort of CKD Stages 3 (25%) and 4 (75%) patients was established from German nephrologist-run CKD clinics in 2013–16. Linear models were used to determine the estimated glomerular filtration rate (eGFR) slope during follow-up and Cox models were used to assess outcomes of end-stage kidney disease (ESKD) and death.

Results
A total of 1834 patients (median age 75 years, 58% male, 42% diabetics, median baseline eGFR 25 mL/min/1.73 m2) were followed for a median of 29 months. More than 50% had slow or no decline and 17% declined ≥5 mL/min/1.73 m2/year. After 4.5 years, the incidence of ESKD was 8% and of deaths without ESKD 16% among patients with eGFR ≥30 mL/min/1.73 m2 and 37% and 19% for eGFR &lt;30 mL/min/1.73 m2. Adjusted models showed higher risks of ESKD or death for patients with worse kidney function at baseline, male sex, diabetes and higher blood pressure; a higher risk of ESKD with higher albuminuria; and a higher risk of death with older age or cardiovascular comorbidity.

Conclusions
Routine nephrology care of patients in Germany comprises mostly elderly patients, many with slow CKD progression. Identification of risk factors for CKD progression and mortality may help guide resources by closer follow-up of high-risk patients.
Mechanical Thrombectomy of Acutely Occluded Flow-Diverters – Neuroendovascular Surgical Technique Demonstration: 2-Dimensional Operative Video
Authors: Roa Jorge A , Hasan David M , Samaniego Edgar A 
Published: November 2019

Abstract
Flow-diversion with pipeline embolization devices (PED, Medtronic, Dublin, Ireland) is widely used for embolization of complex intracranial aneurysms.
1
In-stent thrombosis can be a dreadful complication after PED deployment. Intra-arterial glycoprotein IIb-IIIa inhibitors and intravenous tissue plasminogen activator have been used in an attempt to achieve recanalization.
2
However, large clots may not be effectively dissolved by pharmacological agents, thus requiring mechanical thrombectomy (MT).
3
Our group recently published the first technical report on successful MT of acutely occluded PEDs in 2 patients.
4
Here, we showcase the successful MT of a patient who sustained acute in-stent PED thrombosis. Informed written consent was obtained. In this case, we combined stentriever and contact aspiration thrombectomy techniques. We highlight important pitfalls and tips to prevent PED displacement, removal or vessel injury during endovascular manipulation. The most important consideration is to deploy the distal end of the stentriever inside the PED but also as distally as possible. Thus, correct apposition and alignment of the distal markers of both devices is performed under “native” unsubstracted fluoroscopic view (for better PED visualization) before MT. In this surgical video we describe the technique in detail.
Implementing community-based health promotion in socio-economically disadvantaged areas: a qualitative study
Authors: Lawlor Emma R , Cupples Margaret E , Donnelly Michael , Tully Mark A 
Published: November 2019

Abstract

Background
There is a gradient relationship between socio-economic status and health. We investigated the views and perceptions of health promotion service providers regarding factors that affect lack of engagement in public health initiatives by residents in socio-economically disadvantaged (SED) communities.

Methods
We conducted semi-structured interviews with a purposive sample of key providers (n = 15) of community-based health promotion services to elicit their views about engagement-related factors and their experiences of the provision, delivery and impact of health promotion in SED areas. Interviews were analysed using thematic analysis.

Results
Failure to (i) recognise within SED communities, socio-cultural norms of health-related behaviour and (ii) communicate to local residents an understanding of complex lifestyle influences appeared to affect adversely service engagement and contribute to the development of negative attitudes towards health promotion. Engagement is more likely when services are delivered within familiar settings, peer support is available, initiatives are organized within existing groups, external incentives are offered and there are options regarding times and locations. Collaborative working between providers and communities facilitates efficient, context-sensitive service delivery.

Conclusions
Knowledge of a local community and its socio-environmental context alongside a collaborative, facilitative and tailored approach to delivery are required to ensure successful engagement of SED communities in health promotion.
Is knowledge of screen time guidelines associated with stages of behavior change for television viewing among adolescents?
Authors: da Costa Bruno Gonçalves Galdino , dos Santos Priscila Cristina , Bandeira Alexsandra da Silva , da Costa Rafael Martins , de Sousa Ana Caroline Campos , Da Silva Kelly Samara 
Published: November 2019

Abstract
It is unknown whether knowledge of guidelines for television (TV) viewing translate into behavior change for TV time among adolescents. The purpose of the present study is to verify whether knowledge of TV recommendations (spending &lt;2 hr/day watching TV) is associated with the stages of behavior change related to TV viewing among adolescents. Schoolchildren (11–16 years) from six schools were recruited. Their knowledge of TV guidelines (correct [&lt;2 hr/day], overestimated [&lt;1 hr/day or never watch TV], underestimated [&lt;3hr/day or watch TV whenever they wanted], and do not know [response option]) and stages of behavior change related to TV (precontemplation, contemplation, preparation, action, and maintenance) were assessed by a questionnaire. Multinomial logistic regression was used to test the association between knowledge and stages of behavior change. A total of 792 adolescents (53% girls) participated, of whom 45% were in the precontemplation stage, while 35% underestimated and 18% did not know the TV guidelines. Those who overestimated the guidelines had higher odds of being in the maintenance stage than did those who knew the recommendations correctly (odds ratio [OR] 2.06), while those who underestimated the guidelines had lower odds of being in the maintenance (OR 0.22) and any other stage compared to those who were aware of the recommendations (the precontemplation stage was the reference). Underestimating the guidelines was associated with being in the precontemplation stage, while overestimating them was associated with the maintenance stage. Interventions should test whether increasing knowledge can help shifting those in the precontemplation and contemplation toward action and maintenance.
Pulmonary embolism response team
Authors: Lavender Zachary , MacNabb Megan 
Published: November 2019

ABSTRACT
The Pulmonary Embolism Response Team (PERT) is a multidisciplinary team that quickly evaluates, coordinates diagnosis, and optimizes management for patients with pulmonary embolism (PE), a serious public health problem. The classification and management of PE has evolved over recent years. Despite updated guidelines by major medical organizations, classification and management relies heavily on expert opinion. Given the complexity of patients with PE and the variety of clinical presentations and treatment options, a multidisciplinary team is warranted.