bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic and epigenetic modifications of HPDL and SOX17 associated with breast cancer prognosis: a study based on The Cancer Genome Atlas
Authors: Gao Chundi , Li Huayao , Liu Cun , Wu Jibiao , Zhou Chao , Liu Lijuan , Zhuang Jing , sun Changgang 
Published: March 2020

Abstract
Background：The high heterogeneity of breast cancer (BRCA) makes it more challenging to interpret the genetic variation mechanisms involved in BRCA pathogenesis and prognosis. Areas with high DNA methylation (such as CpG islands) were accompanied by copy number variation (CNV), and these genomic variations affected the level of DNA methylation. Methods: In this study, we characterized inter-tumor heterogeneity and analyzed the effects of CNV on DNA methylation and gene expression. In addition, we performed a Genetic Set Enrichment Analysis (GSEA) to identify key pathways for changes between patients with low and high expression of genes. Results: Our analysis found that the CNV of HPDL and SOX17 is not only related to the patient's prognosis, but also related to gene methylation and expression levels affecting the patient's survival time. Conclusion: This study provided an effective bioinformatics basis for further exploration of molecular mechanisms related to BRCA and assessment of patient prognosis, but the development of biomarkers for diagnosis and treatment still requires further clinical data validation.
Supraphysiological estradiol levels on the hCG trigger day are associated with SGA for singletons born from fresh embryo transfer
Authors: Zhang Junwei , Du Mingze , Wu Yanli , Xiong Yiwen , Wang Yunxia , Li Zhe , Wang Lulu , Hu Jijun , Sun Lijun 
Published: March 2020

Abstract
The effects of supraphysiological estradiol (E2) on neonatal outcomes and the significance of specific E2 concentrations remain unclear. The purpose of this study was investigate whether supraphysiological E2 levels on the hCG trigger day are associated with small size for gestational age (SGA) in singletons born from fresh embryo transfer (ET) cycles. Patients with singleton pregnancies with delivered after transfer of fresh embryos, during the period of July 2012 to December 2017 at our center were included. We excluded cycles involving a vanishing twin, maternal age >35 years, basal FSH ≥10 mIU/ml, AMH ≤1 ng/ml or incomplete records. We then divided all cycles into 5 groups by E2 level on the day of hCG trigger: group A, <2000 pg/ml (reference group); group B, 2000 pg/ml≤E2<2999 pg/ml; group C, 3000 pg/ml≤E2<3999 pg/ml; group D, 4000 pg/ml≤E2<4999 pg/ml; and group E, ≥5000 pg/ml. The prevalence of SGA among singletons from fresh ET was the primary outcome. The SGA rate significantly increased when the E2 level was ≥4000 pg/ml, as observed by comparing groups D (odds ratio [OR]: 1.79, 95% confidence interval [CI]: 1.16–2.76, P=0.01) and E (OR: 1.68, 95% CI: 1.10–2.56, P=0.02) with the reference group. Multivariate logistic regression indicated that a serum E2 level of at least 4000 pg/ml on the hCG trigger day was associated with increased SGA and with significant differences for groups D (adjusted OR [AOR]: 1.65, 95% CI: 1.05–2.59, P=0.03) and E (AOR: 1.60, 95% CI: 1.03–2.53, P=0.04) relative to the reference group. In conclusion，for fresh ET cycles, supraphysiological E2 ≥4000 pg/ml on the hCG trigger day increases the risk of singleton SGA.
Providing calves different feed source early in life would not affect feed sorting and rumen fermentation for a long term
Authors: Xiao Jianxin , Khan Muhammad Zahoor , Alugongo Gibson Maswayi , Wang Wei , Wang Yajing , Cao Zhijun , Li Shengli 
Published: March 2020

Abstract
This study aimed to investigate the short and long-term effect of early in life exposure to different feed sources on feed sorting and rumen fermentation of calves. Forty newborn female Holstein calves were randomly divided based on supplementation of concentrate (CON) or hay (HAY) only during the milk-feeding stage (d 1 to 56). After that, all calves were offered a total mixed ration (TMR) containing 43 % of forage and 57 % of concentrate until the end of the experiment (d 57 to 196). Rectal temperature, feed intake, body weight, body structural measurement, fecal and cough score were recorded ranges from day 1 to 196. Fresh and orts feed were sampled daily at the first two weeks (d 57-70) once TMR was offered and at the last week of the experiment (d 190 to 196) for analysis of feed sorting. Rumen fluid samples were collected at ranges from d 14-190 for detecting rumen pH and volatile fatty acids (VFA) concentrations. Our findings showed that early feed exposure did not influence calf health and growth performance but affected feed sorting in a short period. Upon transition to the same mixed diet, differences were found between treatments in the sorting of the different particle fractions. At week 9 and 10, calves early exposed to concentrates began to sort for fine particle fraction, and against the long particle fraction, whereas calves early fed hay sorted for the long particle fraction, and against the fine particle fraction. However, no carryover effect was found, the differences between treatments fade away when detecting feed sorting at the last week, all calves sorted for short and fine particle fractions, and sort against long and medium particle fractions. Although the pattern of feed sorting initially differed between treatments, the rumen pH and VFA concentration were not altered once all calves transitioned to a TMR. These results suggested that the feed familiarity established early in life would not affect diet selection and rumen fermentation in heifer later in life, the dietary experience after transition to same TMR would eventually override the effect of early feed experience.
Investigation of Potential therapeutic value of Shuanghuanglian Chinese medicine based on network pharmacology for coronavirus pneumonia
Authors: XU JIASHENG , LIAO KAILI , Zhang Guanyu , LI YIRAN , Zhou Weimin , Qiu Jiehua 
Published: March 2020

Abstract
Objective: The interaction network between coronavirus pneumonia and Shuanghuanglian was established to explore the potential therapeutic effect of the active ingredients of Shuanghuanglian on coronary virus pneumonia.Methods: Using the TCMSP database, the effective components of Shuanghuanglian were obtained by screening consistent with oral utilization，drug similarity and blood-brain barrier permeability thresholds, and the drug target prediction was performed.The SARS treatment target mining was performed through the GeneCards database, and the two data sets of therapeutic target and drug target were analyzed and the intersection was screened, and the wayne map was drawn.The intersection genes were used as potential therapeutic targets. Cytoscape 3.6 software was used to build a drug active ingredient-therapeutic target interaction network and analyze the active ingredient-therapeutic target point network Degree parameters to find important active ingredients and targets. Using DAVID and String databases to perform GO,KEGG enrichment analysis and protein interaction analysis on the intersection genes to find out the potential signal pathway of Shuanghuanglian against SARS.Results: 43 effective components against SARS were screened, includingcoptisine、Mandenol、Ethyl linolenate、phytofluene、wogonin、FORSYTHINOL、baicalein、Moslosooflavone、Panicolin, etc.A total of 115 intersection genes with coronavirus pneumonia were screened as important treatment targets: based on the protein interaction network, Shuanghuanglian's therapeutic targets for diseases were significantly enriched in 1763 GO and 133 KEGG signaling pathways; The main action pathways are: responses to steroid hormones and ketones, and development of the reproductive system, responses to lipopolysaccharides, Toxins, responses to bacteria and aging, and effects on epithelial cell proliferation.Conclusion: The active ingredients in Shuanghuanglian Chinese medicine compound can act on the disease-related target of coronavirus pneumonia and have potential therapeutic effects on coronavirus pneumonia.
Is reluctance to share Alzheimer’s disease biomarker status with a study partner a barrier to preclinical trial recruitment?
Authors: Cox Chelsea G , Ryan Mary M , Gillen Daniel L , Grill Joshua D 
Published: March 2020

Abstract
Background: Preclinical Alzheimer’s disease (AD) clinical trials test candidate treatments in individuals with biomarker evidence of AD but no cognitive impairment. Participants are required to co-enroll with a knowledgeable study partner, to whom AD biomarker information will be disclosed.Methods: We performed in-person mixed-methods interviews with 200 cognitively unimpaired older adults to investigate whether reluctance to share biomarker results is associated with viewing the study partner requirement to be a barrier to preclinical AD trial recruitment.Results: Eighteen percent of participants reported strong agreement that the study partner requirement was a barrier to enrollment. Ten participants (5%) agreed at any level that they would be reluctant to share their biomarker result with a study partner. The estimated odds of viewing the study partner requirement as a barrier to enrollment were 26 times higher for these participants (OR=26.3, 95% CI 4.0, 172.3), compared to those who strongly disagreed that they would be reluctant to share their biomarker result. Overall, participants more frequently agreed with positive statements than negative statements about the study partner requirement, including 76% indicating they would want their study partner with them if they learned AD biomarker results.Conclusions: This is one of the first studies to explore how potential preclinical AD trial participants feel about sharing their personal biomarker information with a study partner. Most participants viewed the study partner as an asset to preclinical AD trial enrollment, including having a partner present during biomarker disclosure.
Comparison of the effectiveness and safety of different regimens of tranexamic acid in complex tibial plateau fracture: a retrospective study
Authors: Wang Zhimeng , Lu Yao , Wang Qian , Song Leilei , Ma Teng , Ren Cheng , Li Zhong , Yang Jiarui , Zhang Kun , Zhang Bing 
Published: March 2020

Abstract
Background Previous studies have demonstrated the effectiveness and safety of tranexamic acid (TXA) in orthopedics. However, no study has investigated TXA in complex tibial plate surgery. Therefore, the purpose of this study was to confirm the safety and effectiveness of IV (intravenous) TXA and topical TXA. Material and methods This was a retrospective analysis of prospectively collected data. The control group received an equal amount of placebo (physiological saline solution); the IV group received 1.0 g TXA by intravenous injection before the tourniquet was inflated and before the surgical incision was closed, and the topical group received 3.0 g TXA in 75 mL of physiological saline solution before 5 min prior to the final tourniquet release. Perioperative blood loss, vascular events, wound complications, and adverse reactions were compared for the three groups. Results Baseline data were comparable for all groups. The IV group showed the best results for total blood loss (TBL) and hidden blood loss (HBL) (424.5 ± 29.4 ml, 219.3 ± 33.4 ml, respectively, all p values < 0.001). The topical group performed excellently with regard to postoperative vascular events, wound complications, and adverse reactions, but there was no statistical significant in the incidence of these between the groups. Conclusion This study presents the first information to show that both IV TXA and topical TXA are safe and effective for complex tibial plateau fractures. The IV regimen effectively reduced blood loss during the perioperative period, whereas the topical regimen had a better safety profile.
An apoptosis-related gene signature for the prognosis of hepatocellular carcinoma
Authors: Zhu Pengfei , Lei Zhang , Zhicheng Du , Yuan Liao , Lei Yan , Wenlong Zhai , Kunlun Chen 
Published: March 2020

Abstract
Hepatocellular carcinoma (HCC) is a major public health burden worldwide owning to high incidence and poor prognosis. Although a mushrooming number of apoptosis-related genes had been disclosed in HCC, the prognostic value and clinical utility of them remain to be illustrated. Here, we defined the data from Gene Expression Omnibus (GEO) as a training cohort and data from The Cancer Genome Atlas-Liver Hepatocellular Carcinoma data set (TCGA-LIHC) as a validation cohort. The apoptosis-related differentially expressed genes (AR-DEGs) were identified with the two cohorts and the Gene Set Enrichment Analysis. Then, we constructed a Lasso-penalized Cox regression model using AR-DEGs and conducted a signature including 14 apoptotic genes to calculate the risk score. Patients with a high risk score indicated worse overall survival than those with low risk. Besides, the 3-year and 5-year area under curve (AUC) values of the signature were above 0.7 in both training and validation cohorts (0.762, 0.818, 0.717, 0.745, respectively). Moreover, a nomogram containing the signature and clinical characteristics presented reliable net benefits for the survival prediction. And the nomogram was tested by probability calibration curves and Decision Curve Analysis (DCA). Furthermore, protein-protein interaction (PPI) and Gene Ontology (GO) enrichment analysis disclosed several noticeable pathways that might clarify the hidden mechanism. Collectively, the present study formed a novel signature based on the 14 apoptotic genes and this possibly predicted prognosis and strengthened the communication with HCC patients about the likely treatment.
Effective inhibition of MYC-amplified Group 3 medulloblastoma through targeting translation machinery eIF4F complex
Authors: Zhao Yang , Li Tiantian , Tian Shuaiwei , Meng Wei , Yang Jian , Wang Baocheng , Liang Zhuangzhuang , Zhao Heng , Han Yipeng , Tang Yujie , Ma Jie 
Published: March 2020

Abstract
Background: Medulloblastoma (MB) consists of four major molecular subtypes and group 3 subtype (G3) is the most aggressive one. MYC amplification tends to happen in G3 MB and those patients exhibit the worse prognosis and need novel effective therapeutic approach most urgently. MYC has been shown to be the driver as well as crucial dependency for MYC-amplified G3-MB, thus serving as a promising therapeutic target. Methods: In this study, we tested inhibiting MYC translation in MYC-amplified G3-MB by targeting eIF4F complex. We analyzed multiple MB tumor data sets to identify potential therapeutic target of eIF4F components and utilized genetic or pharmacological approaches for validation. The relevant inhibitory mechanisms were further investigated by Western blot and whole lysate mass spectrometry assays to confirm the targeting of MYC translation. Results: Tumor data set analyses identified eIF4A1, a subunit of eIF4F complex, which was significantly upregulated in G3-MB compared to normal cerebellum and highly correlated with MYC at transcript level. Targeting eIF4A1 with CRISPR/Cas9 approach or small-molecule inhibitor Silvestrol effectively attenuated growth of multiple preclinical models through blocking proliferation and inducing apoptosis. Mechanistically, Silvestrol treatment effectively inhibited MYC expression at protein level and MYC amplification was shown to sensitize MB cells to eIF4A1 inhibition. Moreover, whole proteome mass spectrometry analyses demonstrated additional biological functions or pathways affected by eIF4A1 inhibition, which could help in better understanding its inhibitory mechanisms and revealing combinatory strategies. Conclusion: It is shown in our study that targeting MYC translation by eIF4A1 inhibition could be an effective therapeutic strategy against MYC-amplified G3-MB.
Long non-coding RNA H19 promotes colorectal cancer metastasis via binding to hnRNPAA2B1
Authors: Zhang Yu-Hui , Huang Wei-Bin , Yuan Yu-Jie , Li Jin , Wu Jing , Yu Jie , He Yu-Long , Wei Zhe-Wei , Zhang Chang-Hua 
Published: March 2020

Abstract
Background Long non-coding RNA H19 was demonstrated to be significantly correlated with tumor metastasis. However, the specific functions of H19 in colorectal cancer (CRC) metastasis and the underlying mechanism are still largely unclear. Methods Use public database to screen the potential lncRNA crucial for metastasis in colorectal cancer. The expression of H19 in clinical CRC specimens was detected by qRT-PCR. The effect of H19 on the metastasis of CRC cells was investigated by transwell, wound healing assays, CCK-8 assays and animal studies. The potential proteins binding to H19 was identified by LC-MS and verified by RNA immunoprecipitation (RIP). The expression of indicated RNA and proteins were measured by qRT-PCR or western blot. Results We found the expression of lncRNA H19 was significantly upregulated in primary tumor and metastatic tissues, correlated with poor prognosis in CRC. Ectopic H19 expression promoted the metastasis of colorectal cancer cells in vitro and in vivo , and induced epithelial-to-mesenchymal transition (EMT). Mechanistically, H19 directly bound to hnRNPA2B1. Knockdown of hnRNPA2B1 attenuated the H19-induce migration and invasion in CRC cells. Furthermore, H19 stabilized and upregulated the expression of Raf-1 by facilitated the interaction between hnRNPA2B1 and Raf-1 mRNA, resulting in activation of Raf-ERK signaling. Conclusions Our findings demonstrate the role of H19/hnRNPA2B1/EMT axis in regulation CRC metastasis, suggested H19 could be a potential biomarker to predict prognosis as well as a therapeutic strategy for CRC.
Diagnostic accuracy value of intraoperative frozen section for ovarian neoplasms
Authors: Shahmoradi Farinaz , Arab Maliheh 
Published: March 2020

Abstract
Background Accuracy of intraoperative frozen section diagnosis is extremely important in the evaluation of ovarian tumors so that suitable surgical procedures can be chosen. Methods This retrospective diagnostic accuracy study was conducted in the Department of Obstetrics and Gynecology, Emam Hossein Hospital. The case records of patients with ovarian mass who underwent surgery and intraoperatively frozen section assessments between September 2006 and December 2014 were analyzed. Demographic and clinical data, operative details, frozen section and final histopathology Examination (HPE) were reviewed from the medical records. Results one hundred and ninety three patients with ovarian masses undergoing surgery and frozen section were included. The frozen section diagnoses were benign in 155 (80.31%), borderline in 10 (5.18%), and malignant in 28 (14.51%), whereas the final diagnosis was benign in 154 (79.80%), borderline in 9 (4.66%), and malignant in 30 (15.54%). The overall accuracy of intra-operative frozen section diagnosis was 98.9%. The sensitivity for frozen section diagnosis was 100% for benign, 89% for borderline and 93.3% for malignant category, whereas the specificity was 97%, 99%, and 100%, respectively. There were 3 cases with discordance between the frozen section diagnoses and the final diagnoses, all of which were under diagnosed by frozen section. Conclusion Frozen section was found to be accurate and useful in the intraoperative assessment of patients with ovarian neoplasm. The results may help to determine the type and extent of surgery.
System Life and Reliability Modeling of a Multiple Power Takeoff Accessory Gearbox Transmission
Authors: Wang Kai , Wang Xian-Liang , Zhu Jia-Zan , Ou Dai-Song , Pan Dai-Feng 
Published: March 2020

Abstract
A mathematical model for system life and reliability of a multiple power takeoff aeroengine accessory gearbox transmission was presented. The geometry model of accessory gear train system is distributed to multiple subsystems by the different transmitting powers. The subsystem is composed by three basic gear unit cases: spiral bevel gear unit, single mesh cylindrical gear unit and compound cylindrical gear unit. The lives of each component are combined to determine the unit life. The unit transmission and subsystem transmission interface models are defined to dispose the loads of common components in order to get the subsystem and the total system transmission life. A numerical example of a multiple power takeoff accessory gear train illustrates the use of this program. The initial design could not fulfill the design life requirement, and a design modification was performed to improve the system life and to balance the life of each component. The results of the redesign show that the accessory gear train has a more balanced life distribution by strengthening the weak parts of the transmission, and the overall life of total system is increased above the design requirement. This program can help the designer to approach an optimal accessory gearbox transmission design efficiently.
Development of a CD19 PET tracer for detecting B cells in a mouse model of multiple sclerosis
Authors: Stevens Marc Y , Cropper Haley C , Lucot Katherine L , Chaney Aisling M , Lechtenberg Kendra J , Jackson Isaac M , Buckwalter Marion S , James Michelle 
Published: March 2020

Abstract
Background: B cells play a central role in multiple sclerosis (MS) through production of injurious antibodies, secretion of pro-inflammatory cytokines, and antigen presentation. The therapeutic success of monoclonal antibodies (mAbs) targeting B cells in some but not all individuals suffering from MS highlights the need for a method to stratify patients and monitor response to treatments in real time. Herein, we describe the development of the first CD19 positron emission tomography (PET) tracer and its evaluation in a rodent model of MS, experimental autoimmune encephalomyelitis (EAE).Methods: Female C57BL/6J mice were induced with EAE through immunisation with myelin oligodendrocyte glycoprotein (MOG1–125). PET imaging of naïve and EAE mice was performed 19 h after administration of [64Cu]CD19-mAb. Thereafter, radioactivity in organs of interest was determined by gamma counting, followed by ex vivo autoradiography of central nervous system (CNS) tissues. Anti-CD45R (B220) immunostaining of brain tissue from EAE and naïve mice was also conducted.Results: Radiolabelling of DOTA-conjugated CD19-mAb with 64Cu was achieved with a radiochemical purity of 99% and molar activity of 2 GBq/mol. Quantitation of CD19 PET images revealed significantly higher tracer binding in whole brain of EAE compared to naïve mice (2.02±0.092 vs. 1.68±0.06 percentage of injected dose per gram, %ID/g, p=0.0173). PET findings were confirmed by ex vivo gamma counting of perfused brain tissue (0.22±0.020 vs. 0.12±0.003 %ID/g, p=0.0010). Moreover, ex vivo autoradiography of brain sections corresponded with PET imaging results and the spatial distribution of B cells observed in B220 immunohistochemistry – providing further evidence that [64Cu]CD19-mAb enables visualisation of B cell infiltration into the CNS of EAE mice. Conclusion: CD19-PET imaging can be used to detect elevated levels of B cells in the CNS of EAE mice, and has the potential to impact the way we study, monitor, and treat clinical MS.