bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Relationship Between Lymphocyte Subsets and the Prognosis and Genomic Features of Lung Cancer: A Retrospective Study
Authors: Dai Shuiping , Ren Pengwei , Ren Jing , Yang Lan , Li Weimin 
Published: July 2020

Abstract
Background

It has been shown that the prognosis of malignant tumors was closely related to the composition and function of immune system, which was associated with genomic features. However, the prognostic value of peripheral T lymphocyte subsets and its relationship with genomic features in lung cancer has not been analyzed extensively. Therefore, this study was intended to evaluate the relationship between lymphocyte subsets and the prognosis and genomic features of lung cancer.
Methods

598 lung cancer patients with complete data were included in this study between 2011 and 2018. Kaplan-Meier method and Pearson analyses were conducted to study the prognostic value of CD3+, CD4+, CD8 + T lymphocytes and the rate of CD4/CD8.
Results

Patients with EGFR mutation has lower percentage of CD8 + lymphocytes than patients with EGFR wild-type (24.71 versus 26.62, respectively, P = 0.041). Patients with high CD3 + had better OS than those with low (27 versus 14 months, P = 0.002). OS of higher CD4 + and CD4/CD8 rate had longer OS than of lower (27 versus 12 months, P = 0.002; 25 versus 9 months, P = 0.008, respectively). Patients with high CD8 + had poor PFS than low group (6 versus 11 months, P = 0.009). There was a negative correlation between CD3 + and CD4 + cells and OS in smoking stage 2 female lung cancer patients (PCC = 0.626, P < 0.05; PCC = 0.534, P < 0.05, respectively). In stage 1 male lung cancer patients, CD8 + T cell is negatively correlated with OS and PFS (PCC = 0.295, P < 0.05; PCC = 0.280, P < 0.05, respectively)
Conclusions

Lung cancer patients with EGFR mutation had lower percentage of CD8 + lymphocytes. Lymphocyte subsets might be potential prognostic biomarkers of lung cancer, but they are affected by gender and tumor stage.
The Effects of Scientific Self-efficacy and Cognitive Anxiety on Science Engagement with “Question-Observation-Doing-Explanation” Model during Schools Disruption in COVID-19 Pandemic
Authors: Yang Xiantong , Zhang Mengmeng , Kong Lingqiang , Wang Qiang , Hong Jon-Chao 
Published: July 2020

Abstract
The game-based learning supported by mobile intelligence technology has promoted the renewal of teaching and learning model. Therefore, a model of Question-Observation-Doing-Explanation (QODE) based on smart phones was constructed and applied to the science learning during school disruption in COVID-19 pandemic. In this study, from the theoretical perspective of Cognitive-affective Theory of Learning with Media, Bandura’s Motivation Theory and Community of Inquiry model, self-report measure was used to verify the effect of students’ scientific self-efficacy and cognitive anxiety on science engagement. 357 valid questionnaires were used for structural equation model research. The results of this study indicated that two types of scientific self-efficacy as indicated by scientific leaning ability and scientific learning behavior was negatively associated with cognitive anxiety. In addition, cognitive anxiety was also negatively correlated to four types of science engagement as indicated by cognitive engagement, emotional engagement, behavioral engagement and social engagement through smartphone interactions. These findings provide further evidence for game-based learning promoted by smart phones, contributing to deeper understanding of the associations between scientific self-efficacy, cognitive anxiety and science engagement. The implication of this study pointed out that the QODE model is suitable for implementing at a smart mobile device to students’ science learning.
Serum Metabolomic Signatures Associated with Disease Severity in Allergic Rhinitis
Authors: Xie Shaobing , Zhang Hua , Xie Zhihai , Liu Yongzhen , Gao Kelei , Zhang Junyi , Fan Ruohao , Xie Shumin , Wang Fengjun , Jiang Weihong 
Published: July 2020

Abstract
Background: Allergic rhinitis (AR) is a global healthcare problem with obscure pathogenesis, and few studies have evaluated the association between AR and metabolomics. The aim of this study was to identify differences in serum metabolomics profiling of AR patients compared to healthy controls and to explore novel biomarkers reflecting disease severity.Methods: Serum samples were collected form 29 healthy controls and 72 AR patients, including 30 mild AR patients (MAR) and 42 moderate to severe AR patients (MSAR). Metabolomic profiling was performed by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) in serum samples. Orthogonal partial least square-discriminate analysis (OPLS-DA) was applied to assess the differences between AR patients and controls and for subgroups based on disease severity. Results: These analysis results successfully revealed distinct metabolite signatures which distinguished MAR patients and MSAR patients from health controls. In addition, MSAR patients also could be discriminated from MAR patients basing on their metabolic fingerprints. Most observed metabolite changes were related to pathways associated with glycine, serine and threonine metabolism, pyrimidine metabolism, sphingolipid metabolism, arginine and proline metabolism and fatty acid metabolism. Among these metabolites from dysregulated metabolic pathways, levels of sarcosine, sphingosine-1-phosphate (S1P), cytidine and linoleic acid significantly correlated with AR total nasal symptom score (TNSS) and visual analogue scale (VAS).Conclusions: MSAR patients have a distinctive serum metabolomics profile compared to MAR and health controls. These results suggest that metabolomic profiling may provide novel insights into pathophysiological mechanisms of AR and contribute to its evaluation of disease severity.
Network pharmacology-based active compounds and pharmacological mechanisms of ginseng for depression in post-COVID-19
Authors: Wang Na , Wang Xianlei , He Mengjiao , Zheng Wenxiu , Cai Xiaoqing , Qi Dongmei , Zhang Yongqing , han chunchao 
Published: July 2020

Abstract
Introduction: The novel coronavirus disease 2019 (COVID-19) is in the midst of worldwide panic. Sudden onset of an immediate life-threatening illness, quarantine and unemployment caused by epidemic are all contributors to depression. Ginseng has been reported to be an effective and safe clinical treatment on both immune-regulation and anti-depression. However, the mechanism of its anti-depression effect has not been fully characterized. In order to provide theoretical guidance for further clinical application in post-pandemic, we investigated active compounds and pharmacological mechanisms of ginseng to exert anti-depressant activity using network pharmacology, and discussed the active ingredients with immune-regulation and anti-depression.Methods: Information on compounds in ginseng was obtained from public databases, and genes related to depression were gathered using the GeneCards database. Networks of ginseng-associated targets and depression-related genes were constructed through STRING database. Potential targets and pathway enrichment analysis related to the therapeutic efficacy of ginseng for depression were identified using Cytoscape and Database for Annotation, Visualization and Integrated Discovery (DAVID). Results: Network pharmacological analysis of ginseng in treatment of depression identified 16 active ingredients, 47 potential targets, 32 GO terms, and 8 target gene-regulated major pathways. Among them, kaempferol, beta-sitosterol, stigmasterol, fumarine and frutinone A are bioactive compounds and key chemicals. Core genes in PPI network were AKT1, CASP3, NOS3, TNF, and PPARG. Enrichment results revealed that ginseng could regulate multiple aspects of depression through neuroactive ligand-receptor interaction, HIF-1 signaling pathway, and Serotonergic synapse. More importantly, we found that frutinone A and kaempferol are key ingredients in ginseng with dual activities of immune-regulation and anti-depression. Conclusions: We discovered that the therapeutic activities of ginseng for depression mainly involve neurotransmitters, neurotrophic factors, neurogenesis, HPA axis and inflammatory response. Pharmacological network analysis can help to explain the potential effects of ginseng for treating depression, indicating that ginseng is a preferable herb clinically for immune-regulation and anti-depression in post-pandemic.
Water-soluble chlorophyll-binding proteins from Brassica oleracea allow for stable photobiocatalytic oxidation of cellulose by a lytic polysaccharide monooxygenase
Authors: Dodge N. , Russo D. A. , Blossom B.M. , Singh R.K. , Oort B. van , Croce R. , Bjerrum M. J. , Jensen Poul Erik 
Published: July 2020

Abstract
Background
Lytic polysaccharide monooxygenases (LPMOs) are indispensable redox enzymes used in industry for the saccharification of plant biomass. LPMO-driven cellulose oxidation can be enhanced considerably through photobiocatalysis using chlorophyll derivatives and light. Water soluble chlorophyll binding proteins (WSCPs) make it is possible to stabilize and solubilize chlorophyll in aqueous solution, allowing for in vitro studies on photostability and ROS production. Here we aim apply a WSCP-Chl a as a photosensitizing complex for photobiocatalysis with the LPMO, TtAA9.
Results
We have in this study demonstrated how WSCP reconstituted with chlorophyll a (WSCP-Chl a) can create a stable photosensitizing complex which produces controlled amounts of H2O2 in the presence of ascorbic acid and light. WSCP-Chl a is highly reactive and allows for tightly controlled formation of H2O2 by regulating light intensity. TtAA9 together with WSCP-Chl a shows increased cellulose oxidation under low light conditions, and the WSCP-Chl a complex remains stable after 24 hours of light exposure. Additionally, the WSCP-Chl a complex demonstrates stability over a range of temperatures and pH conditions relevant for enzyme activity in industrial settings.
Conclusion
With WSCP-Chl a as the photosensitizer, the need to replenish Chl is greatly reduced, enhancing the catalytic lifetime of light-driven LPMOs and increasing the efficiency of cellulose depolymerization. WSCP-Chl a allows for stable photobiocatalysis providing a sustainable solution for biomass processing.
Serological Study of Neutralizing Antibodies Against Enterovirus D68 Among Healthy Population in Xiamen, China
Authors: Cheng Tong , Lin Yu , Zhu Rui , Wu Yuanyuan , Xu Longfa , Yin Zhichao , Yang Hongwei , Zheng Qingbing , Que Yuqiong , Liu Che , He Shuizhen , Xia Ningshao 
Published: July 2020

Abstract
Background: Enterovirus D68 (EV-D68) infections poses a serious public health threat, which caused outbreaks of severe respiratory illness and has been closely associated with "polio-like" central nervous system disease. The patterns of immune responses to EV-D68 have not been fully understood. This study was performed to investigate the level of neutralizing antibody against EV-D68 among the healthy population in Xiamen City.Methods: A total of 515 serum samples from healthy people were selected by stratified random sampling in Xiamen City in 2016, and the titer of neutralizing antibody against EV-D68 was detected by an efficient micro-neutralization assay.Results: The overall seroprevalence of neutralizing antibody against EV-D68 was 81.9%, and the overall geometric mean titers (GMT) was 248.0. The positive rates were 42.2%, 49.3%, 71.9%, 94.2% and 98.5%, respectively, in groups of <1, 1-3, 4-6, 7-19 and 20-39 years old, and were up to 100% in the 40-59 and ≥ 60 age groups. The GMTs in these seven groups were 1:39.8, 1:80.6, 1:133.2, 1:283.7, 1:253.3, 1:308.1 and 1:405.0, respectively. The seroprevalence and GMT of EV-D68 showed an increased trend with age, while gender and geographic area didn’t show significant impact.Conclusions: EV-D68 infection was prevalent in Xiamen City. Children are the high-risk groups for EV-D68-related illnesses, especially those aged ≤ 3 years. It is necessary to establish preventive measures and strengthened the surveillance on EV-D68 to prevent disease outbreaks.
Gestão pública e transparente à luz da lei de responsabilidade fiscal: uma análise nos municípios da região CRAJUBAR no período de 2012 a 2015
Authors: Oliveira Antônio Rafael Valério de Oliveira , Cabral Bruno Rodrigues Cabral , Chagas Milton Jarbas Rodrigues Chagas 
Published: July 2020

Objetivo: Verificar nos portais eletrônicos dos municípios do Crato, Juazeiro do Norte e Barbalha (CRAJUBAR), as informações divulgadas acerca da gestão pública em atendimento à lei de responsabilidade fiscal, lei complementar 131/2009 e ao Decreto nº 7185/2010.
Metodologia: Estudo descritivo com abordagem qualitativa. Foram analisados os municípios da Região CRAJUBAR, localizados no sul do Ceará. Os dados utilizados para análise desta pesquisa correspondem ao período de 2012 a 2015 no que se refere aos instrumentos de transparência da gestão fiscal da LRF 101/2000 e o mês de janeiro de 2017 no que se refere à LC 131/2009 e ao Decreto nº 7.185/ 2010. A coleta destes ocorreu por meio dos portais eletrônicos de cada prefeitura. Para análise dos dados coletados, utilizou-se como ferramentas de apoio, a planilha eletrônica Excel versão 2013.
Resultados: Concluiu-se que os gestores municipais das prefeituras pesquisadas não obedecem, na integralidade, à publicação em meio eletrônico dos instrumentos referente à transparência da gestão fiscal, no tocante à divulgação de informações pormenorizadas e em tempo real.
Contribuições do Estudo: Constatou-se a desobediência aos normativos analisados, implicando em uma verificação da eficiência da aplicação da legislação em análise. A evidenciação da transparência é essencial no processo de gestão. Aborda a discussão sobre a transparência dos recursos públicos constante na LRF e na Lei Complementar nº 131/2009, além de demonstrar a discussão sobre a gestão dos recursos em municípios do estado do Ceará.
Evidenciação contábil dos recursos auferidos pelas OSCIPs do nordeste brasileiro: um enfoque nas subvenções e assistências governamentais
Authors: Milton Jarbas Rodrigues Chagas  , Danival Sousa Cavalcante  , Silvana Karina de Melo Travassos  , Suelem Katherinne de Macedo Pinto  , Polyandra Zampiere Pessoa da Silva  
Published: July 2020

Objetivo: Analisar o nível de evidenciação das principais fontes de recursos das Organizações da Sociedade Civil de Interesse Público (OSCIPs) situadas na região Nordeste do Brasil, enfocando as subvenções e assistências governamentais, uma vez que estas devem seguir normas, tais como o pronunciamento CPC 07 (R1) e a norma contábil NBC TG 07 (R2).
Metodologia: A pesquisa se caracteriza como um estudo descritivo, de natureza qualitativa e quantitativa, cujos dados foram coletados das demonstrações contábeis obtidas do website do Ministério da Justiça, referente aos anos de 2010 a 2014.
Resultados: Observou-se que apenas 27% das OSCIPs da região Nordeste evidenciaram as subvenções e assistências em conformidade com o pronunciamento e a norma citados, e que algumas instituições dessa região não publicam suas demonstrações contábeis em todo o período em análise. Além disso, os testes mostram que as OSCIPs da região Nordeste não apresentaram conformidade contábil quanto à evidenciação das subvenções e assistências governamentais auferidas, demonstrando uma necessidade de melhoria do processo de accountability pelas OSCIPs.
Contribuições do estudo: espera-se trazer o debate a respeito da necessidade da elaboração das demonstrações contábeis pelas OSCIPs, bem como da evidenciação de informações que são exigidas pelos CPCs e normas, para que os usuários tenham informações fidedignas sobre a realidade das organizações para o processo de tomada de decisão.
Treatment and analysis of açai berry production waste: a study by the optics of eco-efficiency
Authors: Marcia Athayde Moreira  , Ana Lidia Ferreira de Sousa  
Published: July 2020

Purpose: The main objective of this research was to find out with the açai berry sales establishments of the Belém Metropolitan Region – Pa. (RMB), which treatment has been assigned to wastes (açai berry pits) by the açai beaters, having as analysis parameters the accounting and socio-environmental standpoint, under the optics of PNRS, within a context of eco-efficiency.
Methodology: An exploratory, cross-sectional research was conducted by gathering data of 80 RMB açai production establishments.
Results: The results indicated that the respondents, in part, have knowledge about social and environmental management and about what the social and environmental impacts mean. However, they do not have a solid waste management plan, and, in the perception of the most of them, the obligation to make the proper disposal of açai berry wastes is the responsibility of the city hall, which demonstrates lack of knowledge about the PNRS guidelines. Regarding the treatment of açai berry pits, the majority of them responded that the wastes are destined to landfills, in spite of the possibilities of reuse. When analyzing the treatment of wastes by the accounting-Financial standpoint, it was observed as non-existent, which may be associated to the type of formalization (or its lack) and the fact that they do not have professional support to business management.
Contributions of the Study: This study concluded that it is necessary to establish incentive plans and/or guidances for the reuse of wastes, so that the açai berry beaters can enjoy the financial benefits and contribute in a better way to the reduction of the environmental impacts, performing an eco-efficiency management.
BULLYING AND ITS CORRELATION WITH THE QUALITY OF LIFE OF ADOLESCENTS
Authors: Garbin Clea Adas Saliba , Teruel Gabriela Peres , Costa Adriana Alves , Saliba Tania Adas , Garbin Artênio José Ísper 
Published: July 2020

Objetivou-se verificar a ocorrência e a correlação do bullying com a qualidade de vida de adolescentes. O estudo seguiu os ditames éticos atuais. Trata-se de um estudo transversal, observacional e analítico. Foram visitadas todas as instituições de ensino fundamental (n = 22) de um município do noroeste do estado de São Paulo, Brasil. Todos os alunos (n = 1.068) foram convidados a participar, mas, apenas 382 consentiram. Do total, 3,7% disseram que sempre cometeram bullying; 21,2% afirmaram serem vítimas de intimidação de pares; 11,3% disseram ter uma qualidade de vida nem boa, nem má; 2,4% disseram que suas vidas não fazem sentido. De acordo com os testes de correlação entre o VPAS e o WHOQOL-Bref, houve correlação significativa na interseção dos domínios agressividade direta e agressividade relacional com os domínios físico, psicológico, relações sociais e meio ambiente do WHOQOL-Bref. Conclui-se que a medida que o bullying aumenta, a qualidade de vida diminui.
Palavras- chaves: Bullying; Comportamento do adolescente; Qualidade de vida.
CONDIÇÕES DE SAÚDE E ESTADO NUTRICIONAL DE AGENTES COMUNITÁRIOS DE SAÚDE NO INTERIOR DO NORDESTE BRASILEIRO
Authors: Dantas Amanda Almeida Gomes , De Oliveira Nayara Priscila Dantas , Silva Mercês de Fátima dos Santos , Dantas Diego de Sousa 
Published: July 2020

Introdução: A saúde do trabalhador é um campo de relevância, mas que ainda está sendo consolidado no âmbito do sistema único de saúde (SUS). Partindo dessa compreensão, esse estudo teve como objetivo avaliar o estado nutricional e as condições de saúde dos agentes comunitários de saúde do município de Currais Novos-RN. Metodologia: Participaram da pesquisa 80 indivíduos de ambos os sexos. A coleta de dados envolveu um questionário padronizado elaborado especificamente para o referido trabalho, baseado na Pesquisa Nacional de Saúde, realizada por o IBGE, para analisar as condições de vida e de saúde. Além disso, foram realizadas aferição de medidas antropométricas como peso, estatura, circunferência abdominal e dobras cutâneas, para analisar o estado nutricional. A análise bivariada foi realizada por meio do teste Qui-quadrado de Pearson ou Exato de Fisher, sendo verificada a magnitude da associação através da razão de prevalência com intervalo de confiança de 95%, por meio do programa estatístico SPSS for Windows versão 22.0. Resultados: De acordo com os resultados, viu-se que 63,8% dos participantes encontram-se com Sobrepeso/Obesidade. Conclusão: Todos os dados antropométricos apresentaram diferença significativa na análise bivariada, demonstrando que essas variáveis exercem influência no estado nutricional desses.

Descritores: Agente Comunitário de Saúde; Saúde do Trabalhador; Condições de Saúde; Estado Nutricional.

ABSTRACTIntroduction:Workers' health is a field of relevance, but it is still being consolidated within the scope of the single health system (SUS). Based on this understanding, this study aimed to evaluate the nutritional status and health conditions of community health agents in the municipality of Currais Novos-RN. Methodology: Participated in the study 80 individuals of both sexes. The data collection involved a standardized questionnaire elaborated specifically for the aforementioned study, based on the National Health Survey, conducted by the IBGE, to analyze the living and health conditions. In addition, anthropometric measures such as weight, height, waist circumference, and skinfolds were performed to analyze the nutritional status.The bivariate analysis was performed using the Pearson's Chi-square test or the Fisher's exact test, and the magnitude of the association was verified through the prevalence ratio with a 95% confidence interval, using the statistical program SPSS for Windows version 22.0. Results: According to the results, it was found that 63.8% of the participants they are Overweight / Obesity. Conclusion: And all anthropometric data presented a significant difference in the bivariate analysis, demonstrating that these variables influence the nutritional status of these.
Descriptors: Community Health; Agent Worker’s Health; Health Conditions; Nutritional Status.