bluuebunny/crossref_metadata_2025_split huggingface dataset:
New challenges for efficient usage of Sargassum fusiforme for ruminant production
Authors: Choi You Young , Lee Shin Ja , Lee Ye Jun , Kim Hyun Sang , Eom Jun Sik , Kim Sam Churl , Kim Eun Tae , Lee Sung Sill 
Published: November 2020

AbstractSargassum fusiforme, which is a type of brown algae, can provide fiber and minerals to ruminant diets. In this study, dried S. fusiforme was tested in vitro at four different doses 1, 3, 5, and 10% of the total ration for its effect on ruminal fermentation characteristics, and gas profiles when incubated for 72 h. At a level of 1 and 10%, S. fusiforme supplementation augmented total volatile fatty acid (VFA) concentrations compared to that with 0% supplementation. In addition, total gas, methane, and carbon dioxide emissions significantly decreased at 3 and 24 h of incubation at this dose. An in situ trial was performed for 72 h with S. fusiforme to evaluate it as a potential feed ingredient by comparing its degradation parameters with timothy hay (Phleum pretense). 1H nuclear magnetic resonance spectroscopy profiling was used to identify and quantify metabolites of S. fusiforme. Mannitol, guanidoacetate and ethylene glycol were largely accumulated in S. fusiforme. Moreover, nutritious minerals for feed ingredients were present in S. fusiforme. Whereas a high concentration of arsenic was found in S. fusiforme, it was within the allowable limit for ruminants. Our results suggest that S. fusiforme could represent an alternative, renewable feed ingredient for ruminant diets, with nutritional, as well as environmental, benefits.
LncRNA and mRNA expression profile of peripheral blood mononuclear cells in primary Sjögren’s syndrome patients
Authors: Peng Yu , Luo Xuan , Chen Yingying , Peng Linyi , Deng Chuiwen , Fei Yunyun , Zhang Wen , Zhao Yan 
Published: November 2020

AbstractThe aim of this study was to elucidate the expression profile and the potential role of long non-coding RNA (LncRNA) in the peripheral blood mononuclear cells of primary Sjögren’s syndrome (pSS) patients. RNA-seq technology was used to detect the differentially expressed LncRNAs and mRNAs between five age-and sex-matched paired pSS patients and healthy control PBMCs. The selected LncRNAs were detected in the validation study by RT-qPCR in 16 paired pSS patients and healthy controls. The GO, KEGG, co-localization, and co-expression analysis were performed to enrich the potential gene functions and pathways. In this study, 44 out of 1772 LncRNAs and 1034 out of 15,424 mRNAs were expressed differentially in the PBMCs of pSS patients. LINC00426, TPTEP1-202, CYTOR, NRIR, and BISPR were validated as aberrantly expressed, and these LncRNAs strongly correlated with disease activity of pSS. GO and KEGG pathway analysis revealed the significant enrichment of biological processes, cellular components, and molecular function of the up and down-regulated mRNAs, which were mainly concentrated in the immune response and immune system processes. Co-localization and co-expression analysis also revealed that differentially expressed LncRNAs in the PBMCs of pSS were strongly correlated to the mRNA functioning associated with immune response and cell metastasis. Numerous LncRNAs and mRNAs were found differentially expressed in the PBMCs of pSS patients, especially NRIR and BISPR; they interacted with the co-localized and co-expressed mRNAs, which might participate in the pathogenesis of pSS through the NF-κB, JAK-STAT, and other signaling pathways that regulate cell metastasis.
Human transcription factors responsive to initial reprogramming predominantly undergo legitimate reprogramming during fibroblast conversion to iPSCs
Authors: Cevallos Ricardo R. , Edwards Yvonne J. K. , Parant John M. , Yoder Bradley K. , Hu Kejin 
Published: November 2020

AbstractThe four transcription factors OCT4, SOX2, KLF4, and MYC (OSKM) together can convert human fibroblasts to induced pluripotent stem cells (iPSCs). It is, however, perplexing that they can do so only for a rare population of the starting cells with a long latency. Transcription factors (TFs) define identities of both the starting fibroblasts and the end product, iPSCs, and are also of paramount importance for the reprogramming process. It is critical to upregulate or activate the iPSC-enriched TFs while downregulate or silence the fibroblast-enriched TFs. This report explores the initial TF responses to OSKM as the molecular underpinnings for both the potency aspects and the limitation sides of the OSKM reprogramming. The authors first defined the TF reprogramome, i.e., the full complement of TFs to be reprogrammed. Most TFs were resistant to OSKM reprogramming at the initial stages, an observation consistent with the inefficiency and long latency of iPSC reprogramming. Surprisingly, the current analyses also revealed that most of the TFs (at least 83 genes) that did respond to OSKM induction underwent legitimate reprogramming. The initial legitimate transcriptional responses of TFs to OSKM reprogramming were also observed in the reprogramming fibroblasts from a different individual. Such early biased legitimate reprogramming of the responsive TFs aligns well with the robustness aspect of the otherwise inefficient and stochastic OSKM reprogramming.
Synergistic effects of combinatorial chitosan
and polyphenol biomolecules on enhanced antibacterial activity of biofunctionalized silver
nanoparticles
Authors: Rezazadeh Niloufar Hajarian , Buazar Foad , Matroodi Soheila 
Published: November 2020

AbstractThe present study reports the synergistic antibacterial activity of
biosynthesized silver nanoparticles (AgNPs) with the aid of a combination of chitosan
and seaweed-derived polyphenols as a green synthetic route. Under optimum synthesis
conditions, the rapid color change from yellowish to dark brown and UV–visible
absorption peak at 425 confirmed the initial formation of AgNPs. DLS, TEM, XRD, and EDX
analyses revealed the spherical shape of pure biogenic AgNPs with a mean diameter size
of 12 nm ± 1.5 nm, and a face-centered cubic crystal structure, respectively. FTIR and
TGA results indicated the significant contribution of chitosan and polyphenol components
into silver ions bioreduction and thermal stability of freshly formed AgNPs. Long-term
colloidal stability of AgNPs was obtained after 6-month storage at room temperature. The
bio-prepared AgNPs possessed a negative surface charge with a zeta potential value of
− 27 mV. In contrast to naked chemical silver nanoparticles, the green Ag nanosamples
demonstrated the distinct synergistic antibacterial in vitro toward all selected human
pathogens presumably due to the presence of high content of biomolecules on their
surface. The results show that synergy between chitosan and polyphenol results in the
enhancement of bactericidal properties of biogenic AgNPs. We also highlighted the
underlying mechanism involved in AgNPs formation based on nucleophile–electrophile
interaction.
First-principles identification of the charge-shifting mechanism and ferroelectricity in hybrid halide perovskites
Authors: Kim Bumseop , Kim Jeongwoo , Park Noejung 
Published: November 2020

AbstractHybrid halide perovskite solar cells have recently attracted substantial attention, mainly because of their high power conversion efficiency. Among diverse variants, (CH3NH3)PbI3 and HC(NH2)2PbI3 are particularly promising candidates because their bandgap well matches the energy range of visible light. Here, we demonstrate that the large nonlinear photocurrent in β-(CH3NH3)PbI3 and α-HC(NH2)2PbI3 is mostly determined by the intrinsic electronic band properties near the Fermi level, rooted in the inorganic backbone, whereas the ferroelectric polarization of the hybrid halide perovskite is largely dominated by the ionic contribution of the molecular cation. The spatial charge shift upon excitation is attributed to the charge transfer from iodine to lead atoms in the backbone, which is independent of the presence of the cationic molecules. Our findings can serve as a guiding principle for the design of future materials for halide-perovskite solar cells with further enhanced photovoltaic performance.
Clustering of susceptible individuals within households can drive measles outbreaks: an individual-based model exploration
Authors: Kuylen Elise , Willem Lander , Broeckhove Jan , Beutels Philippe , Hens Niel 
Published: November 2020

AbstractWhen estimating important measures such as the herd immunity threshold, and the corresponding efforts required to eliminate measles, it is often assumed that susceptible individuals are uniformly distributed throughout populations. However, unvaccinated individuals may be clustered in a variety of ways, including by geographic location, by age, in schools, or in households. Here, we investigate to which extent different levels of within-household clustering of susceptible individuals may impact the risk and persistence of measles outbreaks. To this end, we apply an individual-based model, Stride, to a population of 600,000 individuals, using data from Flanders, Belgium. We construct a metric to estimate the level of within-household susceptibility clustering in the population. Furthermore, we compare realistic scenarios regarding the distribution of susceptible individuals within households in terms of their impact on epidemiological measures for outbreak risk and persistence. We find that higher levels of within-household clustering of susceptible individuals increase the risk, size and persistence of measles outbreaks. Ignoring within-household clustering thus leads to underestimations of required measles elimination and outbreak mitigation efforts.
Applying tiling and pattern theory in the design of hollow-core photonic crystal fibers for multi-wavelength beam guidance
Authors: Montz Zev , Ishaaya Amiel A. 
Published: November 2020

AbstractWe apply tiling and pattern theory in the design of hollow-core photonic crystal fibers for guiding light in multiple spectral bandgaps. By combining two different glass apexes in a single [36;32.4.3.4] 2-uniform tiling, transmission regions with fundamental, second and third harmonic wavelengths are supported. This cladding design may also be an excellent candidate for high power beam delivery of Er/Yb, Nd:YAG and Ti:Sapphire laser sources.
Articaine in functional NLC show improved anesthesia and anti-inflammatory activity in zebrafish
Authors: Rodrigues da Silva Gustavo H. , Geronimo Gabriela , García-López Juan P. , Ribeiro Lígia N. M. , de Moura Ludmilla D. , Breitkreitz Márcia C. , Feijóo Carmen G. , de Paula Eneida 
Published: November 2020

AbstractAnesthetic failure is common in dental inflammation processes, even when modern agents, such as articaine, are used. Nanostructured lipid carriers (NLC) are systems with the potential to improve anesthetic efficacy, in which active excipients can provide desirable properties, such as anti-inflammatory. Coupling factorial design (FD) for in vitro formulation development with in vivo zebrafish tests, six different NLC formulations, composed of synthetic (cetyl palmitate/triglycerides) or natural (avocado butter/olive oil/copaiba oil) lipids were evaluated for loading articaine. The formulations selected by FD were physicochemically characterized, tested for shelf stability and in vitro release kinetics and had their in vivo effect (anti-inflammatory and anesthetic effect) screened in zebrafish. The optimized NLC formulation composed of avocado butter, copaiba oil, Tween 80 and 2% articaine showed adequate physicochemical properties (size = 217.7 ± 0.8 nm, PDI = 0.174 ± 0.004, zeta potential = − 40.2 ± 1.1 mV, %EE = 70.6 ± 1.8) and exhibited anti-inflammatory activity. The anesthetic effect on touch reaction and heart rate of zebrafish was improved to 100 and 60%, respectively, in comparison to free articaine. The combined FD/zebrafish approach was very effective to reveal the best articaine-in-NLC formulation, aiming the control of pain at inflamed tissues.
Relationship between cervical elastography and spontaneous onset of labor
Authors: Yo Yoshie , Kotani Yasushi , Shiro Reona , Yamamoto Kiko , Fujishima Risa , Takaya Hisamitsu , Suzuki Ayako , Shimaoka Masao , Matsumura Noriomi 
Published: November 2020

AbstractCervical elastography might be an objective method for evaluating cervical ripening during pregnancy, but its usefulness has not been fully investigated. We examined the significance of cervical elastography in the last trimester of pregnancy. Cervical elastography was performed at weekly checkups after 36 weeks of gestation in 238 cases delivered at our hospital from 2017 to 2018. The correlation with the onset time of natural labor, which is an index for judging maternal delivery preparation status, was examined. A total of 765 examinations were conducted, and cervical stiffness determined by cervical elastography was positively correlated with the Bishop score (r = 0.46, p < 0.0001). When examined separately for each week, only the examinations performed at 39 weeks were associated with the onset of spontaneous labor up to 7 days later (p = 0.0004). Furthermore, when stratified and analyzed by the Bishop score at 39 weeks of gestation, cervical elastography was associated with the occurrence of spontaneous labor pain for up to seven days in the groups with Bishop scores of 3–5 and 6–8 (p = 0.0007 and p = 0.03, respectively). In conclusion, cervical elastography at 39 weeks of pregnancy is useful for judging the delivery time.
The effects of individually designed insoles on pes planus treatment
Authors: Açak Mahmut 
Published: November 2020

AbstractThe aim of this study was to examine the effects of individually designed insole in pes planus treatment. Designed insoles was adjusted according to height, length and function of the sole of each participant with pes planus in order to improve the physical parameters of them. A total of 34 participants (17 males and 17 females) with pes planus participated in the study. Height, weight, percent body fat, 30-m sprint test, vertical jump, 12-min Cooper test and Visual Analog Scale (VAS) measurement were obtained before the study and after 1 year later. Wilcoxon signed rank test was conducted to examine whether there were any differences between the pre- and post-test measurements. It was determined that individually designed insoles reduced body weight and BMI, made positive improvements in 30-m speed, vertical jump and 12-min Cooper scores, and significant decrease in VAS scores. In conclusion, it is seen that individually designed insoles have beneficial role in normalizing forces acting on the foot and improve the physical performance parameters of individuals with pes planus. Future studies are needed to explore the long-term effects of individually designed insoles and prefabricated insoles.
Impact of the severity of restrictive spirometric pattern on nutrition, physical activity, and quality of life: results from a nationally representative database
Authors: Chung Sung Jun , Kim Hwan Il , Yang Bumhee , Kim Taehee , Sim Yun Su , Kang Hyung Koo , Kim Sang-Heon , Yoon Ho Joo , Choi Hayoung , Lee Hyun 
Published: November 2020

AbstractThe general disease burden associated with the restrictive spirometric pattern (RSP) is substantial. However, the impact of RSP by its severity on general health problems and quality of life has not been well elucidated. This study aimed to analyse nutrition, physical activity, and quality of life in subjects who participated in the Korea National Health and Nutrition Examination Survey 2007–2016 according to severity of RSP. Participants were classified as subjects with normal spirometry, those with mild-to-moderate RSP, and those with severe RSP. Poor quality of life was defined as 25th percentile value on the EuroQoL five dimensions (Eq5D) questionnaire index, i.e., 0.90. This study included 23,615 subjects composed of 20,742 with normal spirometry, 2758 with mild-to-moderate RSP, and 115 with severe RSP. The subjects with severe RSP were more likely to have attained lower education levels, had a lower total caloric intake, had less physical activity, had experienced a higher prevalence of comorbidities, and poorer quality of life than those with normal spirometry (P < 0.001 for all). In multivariable analysis, subjects with a mild-to-moderate RSP and severe RSP were more likely to show decreased total calories (coefficient for change in calorie = − 56.6 kcal and − 286.7 kcal, respectively) than those with normal spirometry; subjects with mild-to-moderate RSP and those with severe RSP were 1.26 times and 1.96 times more likely, respectively, to have a poorer quality of life than those with normal spirometry. Additionally, subjects with mild-to-moderate RSP and those with severe RSP were 0.84 times and 0.36 times less likely, respectively, to have high-intensity physical activity than those with normal spirometry in univariable analysis. The trends of a poorer quality of life and physical activity were only significant in the male subgroups. In conclusion, our study revealed that the severity of general health problems and quality of life reductions are correlated with the severity of RSP, especially in males.
Bushveld superplume drove Proterozoic magmatism and metallogenesis in Australia
Authors: Fiorentini Marco L. , O’Neill Craig , Giuliani Andrea , Choi Eunjoo , Maas Roland , Pirajno Franco , Foley Stephen 
Published: November 2020

AbstractLarge-scale mantle convective processes are commonly reflected in the emplacement of Large Igneous Provinces (LIPs). These are high-volume, short-duration magmatic events consisting mainly of extensive flood basalts and their associated plumbing systems. One of the most voluminous LIPs in the geological record is the ~ 2.06 billion-year-old Bushveld Igneous Complex of South Africa (BIC), one of the most mineralised magmatic complexes on Earth. Surprisingly, the known geographic envelope of magmatism related to the BIC is limited to a series of satellite intrusions in southern Africa and has not been traced further afield. This appears inconsistent with the inferred large size of the BIC event. Here, we present new radiometric ages for alkaline magmatism in the Archean Yilgarn Craton (Western Australia), which overlap the emplacement age of the BIC and indicate a much more extensive geographic footprint of the BIC magmatic event. To assess plume involvement at this distance, we present numerical simulations of mantle plume impingement at the base of the lithosphere, and constrain a relationship between the radial extent of volcanism versus time, excess temperature and plume size. These simulations suggest that the thermal influence of large plume events could extend for thousands of km within a few million years, and produce widespread alkaline magmatism, crustal extension potentially leading to continental break-up, and large ore deposits in distal sectors. Our results imply that superplumes may produce very extensive and diverse magmatic and metallogenic provinces, which may now be preserved in widely-dispersed continental blocks.
Normal recognition of famous voices in developmental prosopagnosia
Authors: Tsantani Maria , Cook Richard 
Published: November 2020

AbstractDevelopmental prosopagnosia (DP) is a condition characterised by lifelong face recognition difficulties. Recent neuroimaging findings suggest that DP may be associated with aberrant structure and function in multimodal regions of cortex implicated in the processing of both facial and vocal identity. These findings suggest that both facial and vocal recognition may be impaired in DP. To test this possibility, we compared the performance of 22 DPs and a group of typical controls, on closely matched tasks that assessed famous face and famous voice recognition ability. As expected, the DPs showed severe impairment on the face recognition task, relative to typical controls. In contrast, however, the DPs and controls identified a similar number of voices. Despite evidence of interactions between facial and vocal processing, these findings suggest some degree of dissociation between the two processing pathways, whereby one can be impaired while the other develops typically. A possible explanation for this dissociation in DP could be that the deficit originates in the early perceptual encoding of face structure, rather than at later, post-perceptual stages of face identity processing, which may be more likely to involve interactions with other modalities.
Evaluation of the external validity of a joint structure–function model for monitoring glaucoma progression
Authors: Abu Sampson Listowell , KhalafAllah Mahmoud Tawfik , Racette Lyne 
Published: November 2020

AbstractThe dynamic structure–function (DSF) model was previously shown to have better prediction accuracy than ordinary least square linear regression (OLSLR) for short series of visits. The current study assessed the external validity of the DSF model by testing its performance in an independent dataset (Ocular Hypertension Treatment Study–Confocal Scanning Laser Ophthalmoscopy [OHTS–CSLO] ancillary study; N = 178 eyes), and also on different test parameters in a sample selected from the Diagnostic Innovations in Glaucoma Study or the African Descent and Glaucoma Evaluation Study (DIGS/ADAGES). Each model was used to predict structure–function paired data at visits 4–7. The resulting prediction errors for both models were compared using the Wilcoxon signed-rank test. In the independent dataset, the DSF model predicted rim area and mean sensitivity paired measurements more accurately than OLSLR by 1.8–5.5% (p ≤ 0.004) from visits 4–6. Using the DIGS/ADAGES dataset, the DSF model predicted retinal nerve fiber layer thickness and mean deviation paired measurements more accurately than OLSLR by 1.2–2.5% (p ≤ 0. 007). These results demonstrate the external validity of the DSF model and provide a strong basis to develop it into a useful clinical tool.
Factors associated with extremely poor visual outcomes in patients with central retinal vein occlusion
Authors: Nagasato Daisuke , Muraoka Yuki , Osaka Rie , Iida-Miwa Yuko , Mitamura Yoshinori , Tabuchi Hitoshi , Kadomoto Shin , Murakami Tomoaki , Ooto Sotaro , Suzuma Kiyoshi , Tsujikawa Akitaka 
Published: November 2020

AbstractHere, we examined prognostic factors for extremely poor visual outcomes in patients with central retinal vein occlusion (CRVO) in actual practices. We included 150 consecutive eyes with treatment-naïve acute CRVO from four different facilities and observed them for over 24 months. Macular edema (ME) was treated with one or three monthly anti-vascular endothelial growth factor injections (1 or 3 + pro re nata). According to the final Snellen visual acuity (VA), we divided the patients into very poor VA (< 20/200) and control (≥ 20/200) groups and examined risk factors for poor final visual outcomes. The baseline Snellen VA was hand motion to 20/13. The mean number of anti-VEGF injections for ME was 5.3 ± 3.7 during the follow-up period. In total, 49 (32.7%) patients exhibited a very poor final VA; this group comprised significantly older patients with a significantly poorer baseline VA (P < 0.01 for both) than the control group. Comorbid internal carotid artery disease and diabetic retinopathy were significantly associated with a poor final VA. In actual clinical practice, visual outcomes may be extremely poor despite ME treatment in certain patients with CRVO, with advanced age, poor baseline VA, and comorbid internal carotid artery disease and diabetic retinopathy being significant risk factors.