bluuebunny/crossref_metadata_2025_split huggingface dataset:
The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells
Authors: Andreucci Elena , Peppicelli Silvia , Ruzzolini Jessica , Bianchini Francesca , Biagioni Alessio , Papucci Laura , Magnelli Lucia , Mazzanti Benedetta , Stecca Barbara , Calorini Lido 
Published: August 2020

Abstract
Acidosis characterizes the microenvironment of most solid tumors and is considered a new hallmark of cancer. It is mainly caused by both “aerobic” and “anaerobic” glycolysis of differently adapted cancer cells, with the final product lactic acid being responsible of the extracellular acidification. Many evidences underline the role of extracellular acidosis in tumor progression. Among the different findings, we demonstrated that acidosis-exposed cancer cells are characterized by an epithelial-to-mesenchymal transition phenotype with high invasive ability, high resistance to apoptosis, anchorage-independent growth, and drug therapy. Acidic melanoma cells over-express SOX2, which is crucial for the maintenance of their oxidative metabolism, and carbonic anhydrase IX, that correlates with poor prognosis of cancer patients. Considering these evidences, we realized that the profile outlined for acid cancer cells inevitably remind us the stemness profile. Therefore, we wondered whether extracellular acidosis might induce in cancer cells the acquisition of stem-like properties and contribute to the expansion of the cancer stem cell sub-population. We found that a chronic adaptation to acidosis stimulates in cancer cells the expression of stem-related markers, also providing a high in vitro/in vivo clonogenic and trans-differentiating ability. Moreover, we observed that the acidosis-induced stem-like phenotype of melanoma cells was reversible and related to the EMT induction. These findings help to characterize a further aspect of stem cell niche, contributing to the sustainment and expansion of cancer stem cell subpopulation. Thus, the usage of agents controlling tumor extracellular acidosis might acquire great importance in the clinic for the treatment of aggressive solid tumor.

Key messages
• Extracellular acidosis up-regulates EMT and stem-related markers in melanoma cells
• Acidic medium up-regulates in vitro self-renewal capacity of melanoma cells
• Chronic acidosis adaptation induces trans-differentiation ability in melanoma cells
• Melanoma cells adapted to acidosis show higher tumor-initiating potential than control cells
• Extracellular acidosis promotes a stem-like phenotype in prostate and colorectal carcinoma cells
Causal Reasoning and Meno’s Paradox
Authors: Chen Melvin , Chew Lock Yue 
Published: August 2020

AbstractCausal reasoning is an aspect of learning, reasoning, and decision-making that involves the cognitive ability to discover relationships between causal relata, learn and understand these causal relationships, and make use of this causal knowledge in prediction, explanation, decision-making, and reasoning in terms of counterfactuals. Can we fully automate causal reasoning? One might feel inclined, on the basis of certain groundbreaking advances in causal epistemology, to reply in the affirmative. The aim of this paper is to demonstrate that one still has good skeptical grounds for resisting any conclusions in favour of the automation of causal reasoning. If by causal reasoning is meant the entirety of the process through which we discover causal relationships and make use of this knowledge in prediction, explanation, decision-making, and reasoning in terms of counterfactuals, then one relies besides on tacit knowledge, as might be constituted by or derived from the epistemic faculty virtues and abilities of the causal reasoner, the value systems and character traits of the causal reasoner, the implicit knowledge base available to the causal reasoner, and the habits that sustain our causal reasoning practices. While certain aspects of causal reasoning may be axiomatized and formalized and algorithms may be implemented to approximate causal reasoning, one has to remain skeptical about whether causal reasoning may be fully automated. This demonstration will involve an engagement with Meno’s Paradox.
The outcomes of stand alone polyetheretherketone cages in anterior cervical discectomy and fusion
Authors: Ahmed Abdulaziz F. , Al Dosari Mohammed Al Ateeq , Al Kuwari Abdulaziz , Khan Nasser Mehrab 
Published: August 2020

AbstractThe procedure of anterior cervical discectomy and fusion is considered as the treatment of choice in degenerative disc disease, which material provides the best clinical and radiological fusion and other outcomes remains heavily debated. Materials that augment the process of fusion consist of bone grafting, titanium, polyetheretherketone (PEEK), or carbon cages. The application of PEEK cages has been recommended as it is radiolucent, and it has a modulus of elasticity that is similar to cortical bone. PEEK cages can be either filled with various materials or unfilled cages. Filled PEEK cages can include bone autografts, bone allografts, demineralized bone matrix, and other materials that facilitate fusion. This narrative review highlights that standalone filled PEEK cages were likely to have better radiological outcomes and satisfactory clinical outcomes for myelopathy when compared with standalone unfilled PEEK cages.
Formation of half-period surface relief gratings in azobenzene containing polymer films
Authors: Jelken Joachim , Henkel Carsten , Santer Svetlana 
Published: August 2020

AbstractWe study the peculiar response of photo-sensitive polymer films irradiated with a certain type of interference pattern where one interfering beam is S-polarized, while the second one is P-polarized. The polymer film, although in a glassy state, deforms following the local polarization distribution of the incident light, and a surface relief grating (SRG) appears whose period is half the optical one. All other types of interference patterns result in the matching of both periods. The topographical response is triggered by the alignment of photo-responsive azobenzene containing polymer side chains orthogonal to the local electrical field, resulting in a bulk birefringence grating (BBG). We investigate the process of dual grating formation (SRG and BBG) in a polymer film utilizing a dedicated set-up that combines probe beam diffraction and atomic force microscopy (AFM) measurements, and permits acquiring in situ and in real-time information about changes in local topography and birefringence. We find that the SRG maxima appear at the positions of linearly polarized light (tilted by 45° relative to the grating vector), causing the formation of the half-period topography. This permits to inscribe symmetric and asymmetric topography gratings with sub-wavelength period, while changing only slightly the polarization of one of the interfering beams. We demonstrate an easy generation of sawtooth profiles (blazed gratings) with adjustable shape. With these results, we have taken a significant step in understanding the photo-induced deformation of azo-polymer films.
Controlling orientation, conformation, and biorecognition of proteins on silane monolayers, conjugate polymers, and thermo-responsive polymer brushes: investigations using TOF-SIMS and principal component analysis
Authors: Gajos Katarzyna , Awsiuk Kamil , Budkowski Andrzej 
Published: August 2020

AbstractControl over orientation and conformation of surface-immobilized proteins, determining their biological activity, plays a critical role in biointerface engineering. Specific protein state can be achieved with adjusted surface preparation and immobilization conditions through different types of protein-surface and protein-protein interactions, as outlined in this work. Time-of-flight secondary ion mass spectroscopy, combining surface sensitivity with excellent chemical specificity enhanced by multivariate data analysis, is the most suited surface analysis method to provide information about protein state. This work highlights recent applications of the multivariate principal component analysis of TOF-SIMS spectra to trace orientation and conformation changes of various proteins (antibody, bovine serum albumin, and streptavidin) immobilized by adsorption, specific binding, and covalent attachment on different surfaces, including self-assembled monolayers on silicon, solution-deposited polythiophenes, and thermo-responsive polymer brushes. Multivariate TOF-SIMS results correlate well with AFM data and binding assays for antibody-antigen and streptavidin-biotin recognition. Additionally, several novel extensions of the multivariate TOF-SIMS method are discussed.Graphical abstract
Nitric oxide interacts with cholinoceptors to modulate insulin secretion by pancreatic β cells
Authors: Mussa Bashair M. , Srivastava Ankita , Mohammed Abdul Khader , Verberne Anthony J. M. 
Published: August 2020

AbstractDysfunction of the pancreatic β cells leads to several chronic disorders including diabetes mellitus. Several mediators and mechanisms are known to be involved in the regulation of β cell secretory function. In this study, we propose that cytokine-induced nitric oxide (NO) production interacts with cholinergic mechanisms to modulate insulin secretion from pancreatic β cells. Using a rat insulinoma cell line INS-1, we demonstrated that β cell viability decreases significantly in the presence of SNAP (NO donor) in a concentration- and time-dependent manner. Cell viability was also found to be decreased in the presence of a combined treatment of SNAP with SMN (muscarinic receptor antagonist). We then investigated the impact of these findings on insulin secretion and found a significant reduction in glucose uptake by INS-1 cells in the presence of SNAP and SMN as compared with control. Nitric oxide synthase 3 gene expression was found to be significantly reduced in response to combined treatment with SNAP and SMN suggesting an interaction between the cholinergic and nitrergic systems. The analysis of gene and protein expression further pin-pointed the involvement of M3 muscarinic receptors in the cholinergic pathway. Upon treatment with cytokines, reduced cell viability was observed in the presence of TNF-α and IFN-γ. A significant reduction in insulin secretion was also noted after treatment with TNF-α and IFN-γ and IL1-β. The findings of the present study have shown for the first time that the inhibition of the excitatory effects of cholinergic pathways on glucose-induced insulin secretion may cause β cell injury and dysfunction of insulin secretion in response to cytokine-induced NO production.
Role of c-Fos in orthodontic tooth movement: an in vivo study using transgenic mice
Authors: Decker Maximilian G. , Nottmeier Cita , Luther Julia , Baranowsky Anke , Kahl-Nieke Bärbel , Amling Michael , Schinke Thorsten , David Jean-Pierre , Koehne Till 
Published: August 2020

Abstract
Objectives
The transcription factor c-Fos controls the differentiation of osteoclasts and is expressed in periodontal ligament cells after mechanical stimulation in vitro. However, it is unclear how c-Fos regulates orthodontic tooth movement (OTM) in vivo. The aim of this study was therefore to analyse OTM in transgenic mice with overexpression of c-Fos.

Materials and methods
We employed c-Fos transgenic mice (c-Fos tg) and wild-type littermates (WT) in a model of OTM induced by Nitinol tension springs that were bonded between the left first maxillary molars and the upper incisors. The unstimulated contralateral side served as an internal control. Mice were analysed by contact radiography, micro-computed tomography, decalcified histology and histochemistry.

Results
Our analysis of the unstimulated side revealed that alveolar bone and root morphology were similar between c-Fos tg and control mice. However, we observed more osteoclasts in the alveolar bone of c-Fos tg mice as tartrate-resistant acid phosphatase (TRAP)-positive cells were increased by 40%. After 12 days of OTM, c-Fos tg mice exhibited 62% increased tooth movement as compared with WT mice. Despite the faster tooth movement, c-Fos tg and WT mice displayed the same amount of root resorption. Importantly, we did not observe orthodontically induced tissue necrosis (i.e. hyalinization) in c-Fos tg mice, while this was a common finding in WT mice.

Conclusion
Overexpression of c-Fos accelerates tooth movement without causing more root resorption.

Clinical relevance
Accelerated tooth movement must not result in more root resorption as higher tissue turnover may decrease the amount of mechanically induced tissue necrosis.
Molecular typing tools for identifying and characterizing lactic acid bacteria: a review
Authors: Sharma Anshul , Lee Sulhee , Park Young-Seo 
Published: August 2020

AbstractIdentification and classification of beneficial microbes is of the highest significance in food science and related industries. Conventional phenotypic approaches pose many challenges, and they may misidentify a target, limiting their use. Genotyping tools show comparatively better prospects, and they are widely used for distinguishing microorganisms. The techniques already employed in genotyping of lactic acid bacteria (LAB) are slightly different from one another, and each tool has its own advantages and disadvantages. This review paper compiles the comprehensive details of several fingerprinting tools that have been used for identifying and characterizing LAB at the species, sub-species, and strain levels. Notably, most of these approaches are based on restriction digestion, amplification using polymerase chain reaction, and sequencing. Nowadays, DNA sequencing technologies have made considerable progress in terms of cost, throughput, and methodology. A research journey to develop improved versions of generally applicable and economically viable tools for fingerprinting analysis is ongoing globally.
Age structure in a newly established and increasing population: initially high proportion of young birds among nesting Great Grey Owls
Authors: Sonerud Geir A. , Solheim Roar , Berg Trond 
Published: August 2020

AbstractThe mechanisms behind expansions of the distribution of a bird species and the ensuing establishment of new populations are poorly known. The distribution of Great Grey Owl (Strix nebulosa) in the western Palearctic has generally expanded towards southwest during the past fifty years, and particularly so in Fennoscandia. In the past decade, the recorded breeding population in Norway, confined to Hedmark county bordering Sweden, increased from 1 pair in 2009 to > 100 pairs in 2017–2018, extending the southwestern border of the distribution > 100 km. We studied the age structure of this expanding population based on the molting pattern of the wing feathers of birds captured at the nest site for banding and of non-captured birds photographed in flight. In Fennoscandia the Great Grey Owl relies on shrews and microtine rodents, which usually fluctuate in 3–4 years cycles. The proportion of 1-year old birds among the nesting Great Grey Owls was higher in peak year two of each small mammal population cycle (2011, 2014 and 2018) than in peak year one (2010, 2013 and 2017), and was particularly high (77%) in 2011 when the owl population was far lower (22 nestings recorded) than in later corresponding years (64 nestings in 2014 and 103 in 2018). Thus, this population seems to have been founded to a large extent by birds nesting as 1 year olds, and most likely having dispersed from Sweden. The ability to determine the age of Great Grey Owls without having to capture them extended our data set, in particular for males, which are more reluctant to attack intruders at the nest site and, therefore, less likely to be captured for banding. Being able to age a bird without having to capture it is important, because trapping does not sample a bird population randomly.
A Review of Portable High-Performance Liquid Chromatography: the Future of the Field?
Authors: Rahimi Faraz , Chatzimichail Stelios , Saifuddin Aliyah , Surman Andrew J. , Taylor-Robinson Simon D. , Salehi-Reyhani Ali 
Published: August 2020

AbstractThere is a growing need for chemical analyses to be performed in the field, at the point of need. Tools and techniques often found in analytical chemistry laboratories are necessary in performing these analyses, yet have, historically, been unable to do so owing to their size, cost and complexity. Technical advances in miniaturisation and liquid chromatography are enabling the translation of these techniques out of the laboratory, and into the field. Here we examine the advances that are enabling portable liquid chromatography (LC). We explore the evolution of portable instrumentation from its inception to the most recent advances, highlighting the trends in the field and discussing the necessary criteria for developing in-field solutions. While instrumentation is becoming more capable it has yet to find adoption outside of research.
The importance of habitat diversity and plant species richness for hazel grouse occurrence in the mixed mountain forests of the Western Carpathians
Authors: Matysek Marcin , Gwiazda Robert , Bonczar Zbigniew 
Published: August 2020

AbstractHazel grouse habitat requirements are relatively well known in coniferous forests, and less known in mixed or deciduous forests. We studied habitat differences between sites occupied by hazel grouse Tetrastes bonasia and control plots in mixed mountain forests of the Western Carpathians in 2009 and 2010. Hazel grouse presence at sites was determined in April and May. The habitat variables (n = 21) and the proportion of tree and shrub species (n = 22) were collected both in sites of hazel grouse presence and control plots within a radius of 100 m. Greater numbers of tree species and greater proportions of deciduous trees (mainly birch Betula sp.) were found in sites where hazel grouse was present. Lower canopy cover was an important variable for hazel grouse occurrence, and sites with hazel grouse had a greater proportion of young trees (< 40 years). Sites were also characterized by a higher proportion of overgrown glades and dead woods in comparison with control plots. Sites occupied by hazel grouse were characterized by a greater number of tree species in the undergrowth (minimum of five species) in comparison with control plots. GLM models revealed that the most important environmental factors for hazel grouse occurrence in mixed mountain forests were open habitats (overgrown glades), good hiding opportunities (fallen trees and dead woods) and good conditions for foraging (trees cover in undergrowth). Poplar (Populus sp.) and willow (Salix sp.) were the most important tree species for hazel grouse occurrence. The presence of habitat structures and the vegetations richness provides good shelter or food for the hazel grouse. Extensive forest management should be proposed to increase the number of hazel grouse. Large areas covered by herbs and light-seeded tree species of low economical value for forestry are recommended to support hazel grouse population.
Standards for the performance assessment of territorial landslide early warning systems
Authors: Piciullo Luca , Tiranti Davide , Pecoraro Gaetano , Cepeda Jose Mauricio , Calvello Michele 
Published: August 2020

AbstractLandslide early warning systems (LEWS) can be categorized into two groups: territorial and local systems. Territorial landslide early warning systems (Te-LEWS) deal with the occurrence of several landslides in wide areas: at municipal/regional/national scale. The aim of such systems is to forecast the increased probability of landslide occurrence in a given warning zone. The performance evaluation of such systems is often overlooked, and a standardized procedure is still missing. This paper describes a new Excel user-friendly tool for the application of the EDuMaP method, originally proposed by (Calvello and Piciullo 2016). A description of indicators used for the performance evaluation of different Te-LEWS is provided, and the most useful ones have been selected and implemented into the tool. The EDuMaP tool has been used for the performance evaluation of the “SMART” warning model operating in Piemonte region, Italy. The analysis highlights the warning zones with the highest performance and the ones that need threshold refinement. A comparison of the performance of the SMART model with other models operating in different Te-LEWS has also been carried out, highlighting critical issues and positive aspects. Lastly, the SMART performance has been evaluated with both the EDuMaP and a standard 2 × 2 contingency table for comparison purposes. The result highlights that the latter approach can lead to an imprecise and not detailed assessment of the warning model, because it cannot differentiate among the levels of warning and the variable number of landslides that may occur in a time interval.
Between a bog and a hard place: a global review of climate change effects on coastal freshwater wetlands
Authors: Grieger Rebekah , Capon Samantha J. , Hadwen Wade L. , Mackey Brendan 
Published: August 2020

AbstractCoastal wetlands are significant components of the coastal landscape with important roles in ecosystem service provision and mitigation of climate change. They are also likely to be the system most impacted by climate change, feeling the effects of sea levels rise, temperature increases and rainfall regime changes. Climate change impacts on estuarine coastal wetlands (mangroves, saltmarsh) have been thoroughly investigated; however, the impacts on coastal freshwater wetlands (CFWs) are relatively unknown. To explore the current knowledge of the impacts of climate change on CFWs globally, we undertook a systematic quantitative literature review of peer-reviewed published literature. We found surprisingly little research (110 papers of an initial 678), the majority of which was conducted in the USA, focusing on the effects of sea level rise (SLR) on CFW vegetation or sediment accretion processes. From this research, we know that SLR will lead to reduced productivity, reduced regeneration, and increased mortality in CFW vegetation but little is known regarding the effects of other climate change drivers. Sediment accretion is also not sufficient to keep pace with SLR in many CFWs and again the effects of other climate drivers have not been investigated. The combination of unhealthy vegetation communities and minimal gain in vertical elevation can result in a transition towards a vegetation community of salt-tolerant species but more research is required to understand this process.