bluuebunny/crossref_metadata_2025_split huggingface dataset:
Excised host tissues weight: New Indicators of Total Knee Arthroplasty Worthy of Attention
Authors: Zhu Zhengya , Ruan Ruxin , Bai Chaowen , Yi Linhong , Zhang Le , Pan Sheng , Huang Chaoran , Sun Ziqian , Zheng Xin , Guo Kaijin 
Published: March 2020

Abstract
Background: Knee osteoarthritis (KOA) is a crippling disease and affects many people around the world. Total knee arthroplasty (TKA) is an effective way to treat this disease. The objectaives of this study to compare the weight of the total knee arthroplasty implants and the weight of the natural knee in different groups and also to analyze facts which may affect the weight of surgical host excision. Methods: In total, 105 consecutive TKAs were enrolled in this prospective cohort study with three different brands of knee prostheses. Patients meeting the criterion were divided into the normal bone mass group, the osteopenia group or the osteoporosis group according to the results of preoperative bone density examination. The removal of bone and soft tissues that were collected during TKA operations of each group were weighed and compared with the total weight of the knee prosthesis (including implanted bone cement). Multivariate logistic stepwise regression analysis was conducted to identify indicators affecting host removal weight.Results: The average host removal weight was (132.56±36.88)g for normal bone mass group, (112.86±23.65)g for osteopenia group and (103.74±20.66)g for osteoporosis group. There were statically differences among the three groups. There was a significant difference between the normal bone mass group and the osteopenia group (P=0.003), while no difference between the osteopenia group and the osteoporosis group (P=0.154). No differences were found in the TKA components weight, bone cement weight and knee joint weight gained after TKA among all groups. Stepwise multiple regression analysis using the weight of excised host tissues as a dependent variable and BMD level, gender, age and other available indicators as independent variables revealed that BMD level(B=9.057, p=0.008), gender(B=21.692, p<0.001), Kellgren-Lawrence grade(B=13.790, p=0.003) are significant factors affecting excised host tissues weight. Conclusion: There is a positive correlation between the weight of the host removals of TKA and the patient's bone density. BMD level, gender and K-L stage of KOA were the main factors affecting the weight of the host removal.
Seasonal and Climatic Variation in the Incidence of Adult Acute Appendicitis: A Seven Year Longitudinal Analysis
Authors: York Thomas 
Published: March 2020

Abstract
Introduction Acute appendicitis represents an extremely common surgical emergency, yet its aetiology remains uncertain. A multifactorial understanding of its causation has emerged along with increasing evidence of seasonal variation. This study seeks to find evidence for such a circannual trend within the UK, and further assess key meteorological indicators which may be causative of any such variation. Methods The patient records of a region health body in the North East of England were retrospectively assessed over a 7-year period. Incidence of acute appendicitis was recorded and averaged by month before undergoing statistical analysis for variation and correlation with average temperature, sunlight hours, and rainfall. Results The incidence of acute appendicitis revealed significant seasonal variation with only 38 total cases in the months of January compared to 73 in July, a 92.1% increase. Only a weak correlation was seen between incidence and average sunlight hours/rainfall, however a significant, positive correlation was found between incidence and average temperature ( r=0.58, p=0.48 ). Conclusion Compelling evidence is found to support the existence of a circannual trend for acute appendicitis. Data suggests a seasonal peak in the month of July, accompanied by a low in January, a finding that develops the understanding of this trend from previously equivocal research in the UK.
Assessment of muscular perfusion after oral intake of pre-workout nitric-oxide stimulating supplements by Contrast-Enhanced Ultrasound (CEUS). Study protocol for a placebo-controlled, randomized cross-over trial in healthy young males
Authors: Doll Julian , Bürkle Franziska , Neide Arndt , Tsitlakidis Stefanos , Bruckner Thomas , Schmidmaier Gerhard , Fischer Christian 
Published: March 2020

Abstract
Background The market for dietary supplements in the sports sector has been growing rapidly for several years, though there is still lacking evidence regarding their claimed benefits. One group is that of nitric oxide increasing supplements, so-called "NO-boosters", which are claimed to improve the supply of oxygen and nutrients to the muscle by enhancing vasodilation. The aim of this study was to investigate three of these supplements in healthy male athletes for their muscle perfusion-enhancing potential using contrast-enhanced ultrasound (CEUS).Methods This placebo-controlled, double-blind, randomized cross-over trial will be carried out at the Center for Orthopedics, Trauma Surgery and Spinal Cord Injury of the University Hospital Heidelberg. Three commercial NO enhancing products including 300 mg of the specific green tea extract VASO6™ and a combination of 8 g L-citrulline malate and 3 g L-arginine hydrochloride will be examined for their potential to increase muscular perfusion in 30 male athletes between 18 and 40 years and will be compared with a placebo. On each of the three appointments CEUS of the dominant biceps muscle will be performed at rest and after a standardized resistance training. Every athlete receives each of the three supplements once after a wash-out period of at least one week. Perfusion will be quantified via VueBox® quantification software. The results of CEUS perfusion measurements will be compared intra- and interindividually and correlated with clinical parameters.Discussion The results of this study may help to establish CEUS as a suitable imaging modality for the evaluation of potentially vasodilatory drugs in the field of sports. Other supplements could also be evaluated in this way to verify the content of their advertising claims.Trial registration German Clinical Trials Register (DRKS), ID: DRKS00016972, registered on 25.03.2019.
Enhanced transferrin modified targeting delivery system via AFP promoter MR imaging reporter gene transfection
Authors: Zhou Jiaping , Zhou Qiaomei , Shu Gaofeng , Wang Xiaojie , Lu Yuanfei , Chen Haiyan , Hu Tingting , Cai Jinsong , Du Yongzhong , Yu Risheng 
Published: March 2020

Abstract
Background: Effective treatment and early diagnosis is essential for patients with hepatocellular carcinoma. Nowadays, there is an increasing interest in the utilization of AFP/Fth as an endogenous contrast agent for the early diagnosis of liver cancers. The transfection of AFP/Fth also leads to a considerable upregulation of transferrin receptors (TfR), which might be a potential target for an enhanced delivery of nanomedicines. However, there is no information regarding the utilization of overexpressed TfR for the treatment of liver cancers. Thus, the objective of our study was to investigate whether the transfection of AFP/Fth could be used for the early diagnosis, but also for an enhanced treatment of live cancers. Results: It was found that both of ferritin and TfR were upregulated after the transfection of AFP/Fth plasmid. The transfected cells showed a higher uptake of ferric ion than the un-transfected ones, resulting in a lower T2WI intensity. This result was due to the upregulation of ferritin in transfected cells, suggesting that transfection of AFP/Fth plasmid could be a potential strategy for early diagnosis of liver cancer. As compared with the un-transfected cells, the transfected cells showed a higher uptake of transferrin-modified liposomes, which was due to the specific interaction between transferrin with TfR overexpressed on the transfected cells. This is also the reason why the transferrin modified doxorubicin loaded liposomes (Tf-LP/DOX) showed better in vitro and in vivo anticancer ability than the LP/DOX. This results also suggested that transfection of AFP/Fth could result in an enhanced therapy of liver cancer. Conclusions: Transfection of AFP/Fth could be used for the early diagnosis, but also for an enhanced treatment of live cancers.
Is preoperative vestibular depth a factor affecting shrinkage of free gingival graft: A quasi-experimental study
Authors: Jalali Mahsa , Saeedi Moein , Mohammadi Mohammad , Ranjbar Hadi , Solghar Mohadeseh Arab 
Published: March 2020

Abstract
Background The purpose of the present study was to evaluate the effect of preoperative vestibular depth (VD) on FGG dimensional shrinkage.Methods This study was performed on 20 patients with insufficient attached gingiva width (AGW). Under local anesthesia, a partial thickness bed with submarginal incision was created. An FGG was prepared from the palate and sutured to the prepared bed. Clinical parameters including VD, probing depth (PD), keratinized gingiva width (KGW), AGW, vertical graft dimension (VGD), horizontal graft dimension (HGD) and graft area (GA) were evaluated preoperatively and 1, 3, and 6 months postoperatively. Because the distribution of data was normal, t-test and repeat measurement test were performed to analyze the data.Results Six months after FGG surgery, AGW and KGW raised to 6.15±1.01 mm and 7.55±1.1 mm, which were statistically significant (P <0.001). The shrinkage rate of HGD, VGD, and GA were 13%, 34%, 42% respectively after 6 months. The VD was increased 2.05 mm compared to baseline which was statistically significant (P <0.01). 6 months after surgery, KGW and AGW were 6.95±0.89 mm and 5.62±0.88 mm in patients with baseline VD <9mm, and 8.57±0.53 mm and 7.07±0.34 mm in patients with baseline VD ≥9 mm respectively. The difference of postoperative KGW and AGW between these two groups (based on baseline VD) was statistically significant. (P <0.01, P =0.001 respectively). The decrease in KGW and AGW in patients with VD <9 mm was 1.62 and 1.45 mm respectively after 6 months and this decrease was greater than patients with VD ≥9 mm.Conclusions It was concluded that the shrinkage of FGG was significantly greater in patients with less preoperative VD 6 months after surgery.Trial registration: This study was retrospectively registered by Iranian Registry of Clinical Trials (IRCT) with the number: IRCT20101204005305N18. Registered 22 September 2019. https://irct.ir/trial/41736
The Viewpoints of the Families of Patients with Bipolar I Disorder about the Nature of Disorder and treatment non-compliance: A Qualitative Study
Authors: Mousavi Nasim , norozpour marzieh , Taherifar Zahra , naserbakht Morteza , Shabani Amir 
Published: March 2020

Abstract
Background: Bipolar disorder is a common psychiatric disorder with a great psychological and social burden. Research indicates that therapeutic adherence in these patients is low. On the other hand, family knowledge about the disorder is one of the basic principles of treating this disorder. The purpose of the present study was to investigate the knowledge of family members of patients with bipolar I disorder and the reasons for treatment non-compliance in Iran.Methods: This study was conducted by qualitative content analysis method. In-depth interviews were categorized by open coded inductive method based on common meanings and continuous comparisons into subcategories and main subcategories.Results: The findings of this study indicated that the components of the viewpoints of the families of patients with bipolar I disorder, consisted of five themes: a) information about the disorder, b) information about the medications, c) information about the treatment and the role of the family in it, d) causes of patient’s medication non-compliance, and e) family guidelines for patient’s medication compliance.Conclusions: This study showed that family members did not have enough information about the nature of bipolar I disorder and related this to the lack of education. They did not know what causes the recurrence of the disorder and did not have sufficient knowledge about the prescribed drugs and treatment. Also, most families did not know the etiology of the disorder. Lack of knowledge among families can have a significant impact on relapse and therapeutic non-compliance of patients. It seems that these points need to be further emphasized in the education of families. The results of this study will be used to design guidelines and protocols for relapse prevention and family education and reduce the costs.
Association Between Genetic Polymorphisms and Steady-State Plasma Concentration of Warfarin Optical Isomers
Authors: Liu Yiwei , Chen Quanyao , Ming Wai-Kit , Wang Feiyu , Lin Rongfang , Lin Cuihong , Huang Pinfang 
Published: March 2020

Abstract
Background We evaluated the effects of CYP2C19*2, CYP2C9*3, VKORC1 A1639G, CYP4F2, and MDR1 C3435T gene polymorphisms on the plasma concentrations of R- and S-warfarin enantiomers at the same dose.Methods The plasma concentrations of R- and S-warfarin were determined by ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) in 136 patients. The PCR-RFLP genotyping results were verified by pyrosequencing. The Hardy–Weinberg equilibrium of genotype frequencies was assessed using the Chi-square analysis. Relationships between genotype and plasma concentrations of the enantiomers were analyzed by Kruskal–Wallis test.Results There was no significant difference in the dosage between groups (P > 0.05). The CYP2C19*2, CYP2C9*3, CYP4F2, MDR1 C3435T, and VKORC1 A1639G mutation frequencies were 37.5%, 6.25%, 19.12%, 31.25%, and 3.31%, respectively. The plasma concentrations were non-normally distributed. The S-warfarin plasma concentration was significantly higher in CYP2C9*3 carriers than in non-carriers (P = 0.018) and in patients carrying the T allele of CYP4F2 than those carrying the C allele (P = 0.03). The VKORC1 A1639G polymorphism did not affect the steady-state plasma concentrations of R- and S-warfarin. The enantiomer ratio in homozygous patients (GG) was significantly lower than that in heterozygous patients (GA) and those lacking the mutation (AA) (P = 0.039). Enantiomer plasma levels were not significantly different between MDR1 C3435T and CYP2C19*2 (P > 0.052).Conclusions The CYP2C9*3 and CYP4F2 mutations are associated with increased plasma concentrations of S-warfarin. The VKORC1 A1639G polymorphism might affect the plasma ratio of R- and S-warfarin. Maintenance dose reduction during warfarin administration can be considered for patients with the CYP2C9*3 and CYP4F2 mutations.
High efficient extracellular production of recombinant Streptomyces PMF phospholipase D in Escherichia coli
Authors: Wang Jing , Xu Sheng , Pang Yang , Wang Xin , Chen Kequan , Ouyang Pingkai 
Published: March 2020

Abstract
Background Currently, Streptomyces is widely used in the preparation of phospholipase D (PLD) with high transphosphatidylation activity. However, the yield of PLD from Streptomyces was low and the culture period was long. Therefore, an efficient and cost-effective method is needed urgently.Results Firstly, PLDs from Streptomyces PMF and Streptomyces racemochromogenes were separately over-expressed in E. coli to compare their transphosphatidylation activity based on the synthesis of phosphatidylserine (PS), and PLDPMF was determined to have higher activity. To further improve PLDPMF synthesis, a secretory expression system suitable for PLDPMF was constructed and optimized with diﬀerent signal peptides. The highest secretory efficiency was observed when the PLDPMF gene was expressed together with its native signal peptide (Nat) and the signal peptide PelB from E. coli. For the application of recombinant PLD to PS synthesis, the PLD properties were characterized and 30.2 g/L of PS was produced after 24 h of bioconversion when 50 g/L phosphatidylcholine (PC) was added.Conclusions We succeeded in over-expressing PLD from Streptomyces PMF in E. coli with high transphosphatidylation activity and enhanced the yield by secretory expression. The secreted PLD was successfully used in the production of PS. Our work makes the large-scale production of PLD and PS feasible.
Roles and functions of exosomal miRNA in abdominal aortic aneurysm
Authors: Zhao YuBo , Wan Shuwei , Li Chuang , Guo Yaming , Cai Gaopo , Jiao Zhouyang , Hua Zhaohui , Xu Peng , Liu Shirui , Cao Hui , Li Zhen 
Published: March 2020

Abstract
Background and objective: abdominal aortic aneurysms（AAAs）are the permanent dilatation of the abdominal aorta, ruptured AAA is a serious threat to the patient's life. It’s hardly known about exosomal miRNAs in AAA. The main purpose of this article is to screen miRNAs which differentially expressed in exosomes from normal people and patients with AAA, and to understand the mechanism of work.Material and methods: The plasma of healthy control group and patients with AAA were collected, and the RNAs from exosomes was isolated and sequenced. The mature miRNA sequence of miRBase 21 database was used to identify the known type and expression of miRNAs. DEGseq software was used to analyze the types of miRNAs with significant difference between the experimental group and the control group ( P ＜ 0.05, | log 2 (FoldChange) | ≥ 1 ). The targets of miRNAs were detected by miRTarBase, miRDB, TargetScan and miRWalk software. Targets were analyzed based on Kyoto encyclopedia of genes and genomes（ KEGG ）biological pathway and gene ontology（ GO ）functional enrichment analysis. Conclusion: miRNAs in exosomes regulate in the progress of AAA by activating PI3K-Akt/mTOR and MAPK pathway, and its mechanism needs more research. Background and objective: abdominal aortic aneurysms（AAAs）are the permanent dilatation of the abdominal aorta, ruptured AAA is a serious threat to the patient's life. It’s hardly known about exosomal miRNAs in AAA. The main purpose of this article is to screen miRNAs which differentially expressed in exosomes from normal people and patients with AAA, and to understand the mechanism of work. Material and methods: The plasma of healthy control group and patients with AAA were collected, and the RNAs from exosomes was isolated and sequenced. The mature miRNA sequence of miRBase 21 database was used to identify the known type and expression of miRNAs. DEGseq software was used to analyze the types of miRNAs with significant difference between the experimental group and the control group ( P ＜ 0.05, | log 2 (FoldChange) | ≥ 1 ). The targets of miRNAs were detected by miRTarBase, miRDB, TargetScan and miRWalk software. Targets were analyzed based on Kyoto encyclopedia of genes and genomes（ KEGG ）biological pathway and gene ontology（ GO ）functional enrichment analysis.Conclusion: miRNAs in exosomes regulate in the progress of AAA by activating PI3K-Akt/mTOR and MAPK pathway, and its mechanism needs more research.
Human and spatial-temporal clustering analysis of human brucellosis in mainland China from 2012 to 2016
Authors: Liang Peifeng , Zhao Yuan , Zhao Jianhua , Pan Dongfeng , Guo Zhongqin 
Published: March 2020

Abstract
Objective: The aim of the present study was to better understand the epidemiology of brucellosis in mainland China. We set out to investigate the human, temporal and spatial distribution and clustering characters of the disease.Methods: Human brucellosis data from mainland China between 2012 and 2016 were collected from the China Information System for Disease Control and Prevention. A geographic information system ArcGIS10.3 (ESRI, Redlands) and SaTScan software were used to identify potential changes in the spatial and temporal distribution of human brucellosis in mainland China during the study period.Results: A total of 244,348 cases of human brucellosis were reported during the study period. During 2012-2016, the average incidence of human brucellosis was higher in the 40-60 age group. The temporal clustering analysis showed that the high incidence of brucellosis occurring between march and July annually. The spatial clustering analysis of the incidence of human brucellosis showed that the location of brucellosis clustering in mainland China remained relatively fixed, mainly concentrated in most parts of northern China. The results of spatial-temporal clustering analysis showed that the primary cluster located in northeast of China, including Inner Mongolia, Heilongjiang, Jilin and Liaoning, and the high-risk time was from January 2012 to December 2013.Conclusion: Human brucellosis remains a widespread challenge, particularly in northern China. The clustering analysis highlights potential high-risk human, time and areas which may require special plans and resources for monitoring and controlling the disease.
Order-statistic filtering Fourier decomposition and its applications to rolling bearing fault diagnosis
Authors: Huang Siqi , Zheng Jinde , Pan Haiyang , Tong Jinyu 
Published: March 2020

Abstract
Inspired by the empirical wavelet transform (EWT) method, a new method for nonstationary signal analysis termed order-statistic filtering Fourier decomposition (OSFFD) is proposed in this paper. The OSFFD method uses order-statistic filtering and smoothing to preprocesses the Fourier spectrum of original signal, which improves the problem of sometimes unreasonable boundaries obtained by EWT directly segmenting the Fourier spectrum. Then, the mono-components with physical significance are obtained by adaptively reconstructing the coefficient of fast Fourier transform in each interval, which improves the problem of too many false components obtained by Fourier decomposition (FDM). The OSFFD method also is compared with the existing nonstationary signal decomposition methods including empirical mode decomposition（EMD）, EWT, FDM and variational mode decomposition（VMD） through analyzing simulation signals and the result indicates that OSFFD is less affected by noise and is much more accurate and reasonable in obtaining mono-components. After that, the OSFFD method is compared with the mentioned methods in diagnostic accuracy through analyzing the tested faulty bearing vibration signals and the effectiveness and superiority of OSFFD to the comparative methods in bearing fault identification are verified.