bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fast Fluorine-18 Labeling and Preclinical Evaluation of Novel Mucin1 and its Folate Hybrid Peptide conjugate for Targeting Breast Carcinoma.
Authors: aljammaz ibrahim , Al-Otaibi B. , Al-Malki Y. , Abousekhrah A. , Okarvi S. M. 
Published: July 2020

Abstract
Background: There is a need to develop new and more potent radiofluorinated peptide and their hybrid conjugates for multiple-receptors targeting properties that overexpress on many cancers.Methods: We have synthesized MUC1-[18F]SFB and MUC1-FA-[18F]SFB hybrid conjugates using a convenient and one-step nucleophilic displacement reactions. In vitro cell binding and in vivo evaluation in animals were performed to determine the potential of these radiolabeled compounds.Results: Radiochemical yields for MUC1-[18F]SFB and MUC1-FA-[18F]SFB conjugates were greater than 70% in less than 30 min synthesis time. Radiochemical purities were greater than 97% without HPLC purification, which makes these approaches amenable for automation. In vitro studies on MCF7 breast cancer cells showed that the significant amounts of the radiofluorinated conjugates were associated with cell fractions and held good affinity and specificity for MCF7 cells. In vivo characterization in Balb/c mice revealed rapid blood clearance with excretion predominantly by urinary as well as hepatobiliary systems for MUC1-[18F]SFB and MUC1-FA-[18F]SFB, respectively.Biodistribution in SCID mice bearing MCF7 xenografts, demonstrated excellent tumor uptake (12% ID/g) and favorable kinetics for MUC1-FA-[18F]SFB over MUC1-[18F]SFB. The tumor uptake was blocked by the excess co-injection of cold peptides suggesting the receptor-mediated process.Conclusion: Initial PET/CT imaging of SCID mice with MCF7 xenografts, confirmed these observations. These results demonstrate that MUC1-FA-[18F]SFB may be a useful PET imaging probe for breast cancer detection and monitoring tumor response to the treatment.
Rhythmic Control of The Plant-Microbiome Holobiont
Authors: Lu Tao , Zhang Zhenyan , Li Yan , Zhang Qi , Sun Liwei , Peijnenburg W.J.G.M. , Peñuelas Josep , Zhu Lizhong , Zhu Yong-Guan , Chen Jianmeng , Qian Haifeng 
Published: July 2020

Abstract
Background: Many physiological and metabolic processes in plants are modulated by a circadian clock. Rhizospheric microorganisms fed by root exudates provide beneficial functions to their plant host. The intricate connection between the circadian clock and the rhizospheric microbial community remains poorly understood. Results: We investigated the role of the Arabidopsis circadian clock in shaping the rhizospheric microbial community using wild-type plants and clock mutants (cca1-1 and toc1-101). We performed transcriptomic and metabolomic analyses and sequenced amplicons of the 16S ribosomal RNA gene to characterize gene transcription, root exudation and the bacterial communities, respectively, throughout the day (24 h). Deficiencies of the central circadian clock led to abnormal diurnal rhythms for thousands of expressed genes and dozens of root exudates. Bacterial community failed to follow obvious patterns in the 24-h period, and lack of coordination with plant growth in the clock mutants. Conclusions: Our findings suggested that the biological clock was an important force that drove plants to adjust their rhizospheric microbiomes for adapting to different growth stages.
An ADePT Evaluation for Incorporating the TIPPS Periodontal Health Intervention Into Primary Care Ante-Natal Programmes to Enhance Infant Birthweight in Palestine: A Feasibility Study
Authors: Abuhaloob Lamis , Helles Nahla , Mossey Peter , Freeman Ruth 
Published: July 2020

Abstract
Background: A feasibility study was conducted to implement the TIPPS intervention for pregnant women to enhance infant birthweight in a conflict area in a LMIC. The decision tool, ADePT examines the methodological factors, identified in a feasibility study, that may require modification for a full trial. Objectives: To use the ADePT decision tool to evaluate if the feasibility study had achieved its objectives and to identify the need for intervention, clinical context and trial design modification.Basic research design: Feasibility study: a one-arm, pre-test-post-test design. The ADePT framework was applied to evaluate the findings from the feasibility study.Clinical setting: Primary healthcare clinic located in Gaza City, Palestine. Participants: 25 pregnant women in their first trimester and clinic staff.Interventions: The TIPPS periodontal health intervention delivered by ante-natal nurses.Main outcome measures: ADePT checklist: sample size estimation, recruitment, consent, intervention adherence, intervention acceptability, costs and duration, completion and appropriateness of outcome assessments, retention, logistics and synergy between protocol components.Results: 25 pregnant women attended the baseline and 22 completed the second follow-up appointment. Data acquisition of the primary and secondary outcomes was mainly successful, the adherence and acceptability of the intervention was noted. The participants stated the value of the intervention. The clinic staff voiced concerns regarding time and costs of nurses providing the TIPPS intervention. This allowed suggestions to be made regarding modification of trial design and context of implementation. Conclusions: The ADePT evaluation showed it was possible to progress to full trial with modifications in trial design.
Personalized Hypertension Management Based on Serial Assessment and Telemedicine: a Cluster Randomize Controlled Trial Protocol in Anhui, China
Authors: Xiao Siyi , Shen Xingrong , Liu Rong , Tong Guixian , Liu Tongzhu , Wang Debin 
Published: July 2020

Abstract
Background: Despite tremendous investment worldwide, hypertension treatment and control rates remain low, suggesting that contemporary population-based hypertension management may have reached its efficacy ceiling. The complexity and long-term dynamics of influencing factors make personalized management inevitable and challenging. This protocol describes Personalized Hypertension Management in Anhui, China (PHMA), a project that uses a package of innovative approaches in tailoring interventions to individual patient’s dynamic complications and contexts.Methods/design: PHMA strives to reduce hypertension harms by eight proven “objective behaviors” (e.g., self-monitoring and reporting, healthy diet, physical exercise/activities). These objective behaviors are promoted through five intervention measures: support for self- monitoring, supervised machine communications, daily education or reminder messages, weekly blood pressure notification, and quarterly signed feedback. PHMA uses ten categories and over 300 variables in selecting and refining intervention procedures and content for individual patients. Efficacy of the intervention package is evaluated using a cluster randomized controlled trial design involving a total of 60 site communities and 3352 hypertension patients. Primary measure for the evaluation is systolic and diastolic blood pressure; while secondary evaluation measures include quality of life (EQ5D-5L), occurrence of hypertension-related complications (such as cerebral hemorrhage, coronary heart disease, myocardial or cerebral infarction), healthcare utilization and scores of objective behaviors.Discussion: PHMA uses novel, low cost and sustainable approaches to tailor interventions to the dynamic conditions and contexts of individual patients. Unlike contemporary approaches to hypertension management which are mainly population based, each participant patient in PHMA applies a unique intervention package and all messages, feedbacks and other materials sent out to individual patients are different from each other. PHMA is the first project that adopts so comprehensive tailoring and if proved effective, it should have important implications for future research, practice and policy-making.Trial registration: Registered July 17, 2020. ISRCTN10999269.
How do patient feedback systems work in low- and middle-income countries? Insights from a realist evaluation in Bangladesh.
Authors: Mirzoev Tolib , Kane Sumit , Azdi Zunayed Al , Ebenso Bassey , Chowdhury Ayesha Afroz , Huque Rumana 
Published: July 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Role of community pharmacies in a population-based colorectal cancer screening program: opportunities for quality improvement
Authors: Vives Nuria , Milà Nuria , Binefa Gemma , Travier Noemie , Farre Albert , Vidal Carmen , Sattari Maryam , Bagaria Guillermo , Garcia Montse 
Published: July 2020

Abstract
Background

In Catalonia (Spain), most CRC screening hubs use the pharmacy-based model to distribute and collect the fecal immunochemical test (FIT) kits. The comprehensive evaluation of CRC screening programs, which include the role and implications of pharmacy involvement, is essential to ensure program quality and identify areas for further improvement. The present study aimed to analyze pharmacy collaboration with the screening program and pharmacy-based FIT kit distribution.
Methods

A descriptive study to describe the role of community pharmacies as well as the FIT distribution and collection data during 2018 was conducted. Time to FIT completion was assessed by Kaplan-Meier estimation, and with the log-rank test. A Cox regression model was used to adjust for other variables associated with the completion of FIT such as sex, age, deprivation score and previous screening behavior.
Results

Overall, 82.4% of pharmacies collaborated with CRC screening program. Out of 82,902 FIT kits distributed to screening invitees 77,524 completed FIT kits were returned to pharmacies (93.5%) with a participation of 39.8% among the 193,766 invitees. From those who completed a FIT, the median time to return the kit was 3 days. FIT completion time was significantly lower among women, older age, high deprivation score and previous CRC screening (p < 0.005).
Conclusions

In our cancer screening setting the engagement of community pharmacies is high. Our findings suggest that community pharmacists as part of an integrated screening program team and by providing key screening process data can enable a better understanding of CRC screening behavior.
Evaluation of the nephrotoxicity and safety of low-dose aristolochic acid, extending to the use of Xixin (Asurum), by determination of methylglyoxal and D-lactate
Authors: Lin Chia-En , Lin Po-Yeh , Yang Wen-Chi , Huang Yu-Shen , Lin Tzu-Yao , Chen Chien‐Ming , Chen Hung-Shing , Lee Jen-Ai , Chen Shih-Ming 
Published: July 2020

Abstract
Background: Most Aristolochiaceae plants are prohibited due to aristolochic acid nephropathy (AAN), except Xixin ( Asarum spp. ). Xixin contains trace amounts of aristolochic acid (AA) and is widely used in Traditional Chinese Medicine. Methylglyoxal and D-lactate are regarded as biomarkers for nephrotoxicity. Thus, this study aimed to evaluate tubulointerstitial injury and interstitial renal fibrosis by determining urinary methylglyoxal and D-lactate after withdrawal of low-dose AA in a chronic mouse model. Methods: C3H/He mice in the AA group ( n =24/group) were given ad libitum access to distilled water containing 3 µg/mL AA (0.5 mg/kg/day) for 56 days and drinking water from days 57 to 84. The severity of tubulointerstitial injury and fibrosis were evaluated using the tubulointerstitial histological score (TIHS) and Masson’s trichrome staining. Urinary and serum methylglyoxal were determined by high-performance liquid chromatography (HPLC); urinary D-lactate were determined by column-switching HPLC. Results: After AA withdrawal, serum methylglyoxal in the AA group increased from day 56 (429.4±48.3 μg/L) to 84 (600.2±99.9 μg/L), and peaked on day 70 (878.3±171.8 μg/L; p <0.05); TIHS and fibrosis exhibited similar patterns. Urinary methylglyoxal was high on day 56 (3.522±1.061 μg), declined by day 70 (1.583±0.437 μg) and increased by day 84 (2.390±0.130 μg). Moreover, urinary D-lactate was elevated on day 56 (82.10±18.80 μg) and higher from day 70 (201.10±90.82 μg) to 84 (193.28±61.32 μg). Conclusions: Methylglyoxal is induced after AA-induced tubulointerstitial injury, thus methylglyoxal excretion and metabolism may be a detoxification and repair strategy. A low cumulative AA dose is the key factor that limits tubulointerstitial injury and repair. Thus, AA-containing herbs, especially Xixin, should be used at low doses for short durations (less than one month).
Neural Processing of Speech Sounds in ASD and First-Degree Relatives
Authors: Patel Shivani P. , Winston Molly , Guilfoyle Janna , Nicol Trent , Martin Gary E. , Kraus Nina , Losh Molly 
Published: July 2020

Abstract
Background: Efficient neural encoding of sound plays a critical and widespread role in speech and language, and when impaired, may have reverberating effects on a range of communication skills. Autism spectrum disorder (ASD) is a condition involving impaired communication abilities, including atypical prosody (e.g., intonation modulation, rate, rhythm), in which atypical neural processing of speech has been implicated. Parallel patterns of communication differences have been noted in parents of individuals with ASD, who may exhibit subclinical language-related differences believed to reflect genetic liability to ASD. The present study investigated disruptions to neural processing of temporal and spectral (i.e., frequency/pitch) properties of speech sounds as a potential neurobiological mechanism underlying specific language-related impairments in ASD and related subclinical differences documented among first-degree relatives of individuals with ASD. Methods: Participants included individuals with ASD, their parents, and respective control groups. Group differences in temporal and spectral processing based on the neural FFR were assessed using MANCOVAs controlling for chronological age. Relationships between the FFR, pragmatic language ability, and receptive and expressive prosody skills were assessed using Pearson correlations. Familiality of the FFR in mother-child dyads were also examined using Pearson correlations.Results: Findings revealed inefficiencies in neural encoding of speech sounds in both individuals with ASD and their parents (specifically, increased neural noise and delayed neural processing of speech sounds), as well as a less robust neural representation of spectral properties of speech sounds in individuals with ASD. Associations between neural processing of speech sounds and language-related abilities were detected in both groups, along with evidence of familiality of neural pitch processing in the ASD family groups. Limitations: Additional investigation is necessary to determine whether relationships between FFR and pragmatic language and prosody extend to individuals with more severe language and/or cognitive impairments. Conclusions: Overall, results suggest atypical neural processing of speech sounds may constitute an important, heritable ingredient contributing to the ASD language phenotype and subclinical phenotypic expression of genetic liability in parents.
First Report of Thelazia Callipaeda Infection in Amiota Okadai and Wildlife in Wildlife Home Range, China
Authors: Jin Yipeng , Liu Zichen , Wei Jiaqi , Wen Yifan , He Nianjun , Tang Liubin , Lin Degui , Lin Jiahao 
Published: July 2020

Abstract
Background: Thelazia callipaeda is a nematode of the sucking nematode genus of the sucking family, with Amiota okadai as the intermediate host and vector in China. China has the largest number of cases of thelaziosis in humans in the world. It is generally believed that domestic animals (dogs and cats) are the most important reservoir host of Thelazia callipaeda and directly threaten humans. At present, there is not much research and attention on the role of wildlife in the transmission cycle of thelaziosis in wildlife home range.Methods: During 2016-2019, we selected four wildlife national nature reserve across the country as monitoring points for Amiota okadai and wildlife. And we chose to use fly-trap method for monitoring Amiota okadai density. Morphological analysis of the parasites collected from the conjunctival sac of the wildlife was taken as the first step, and a specific PCR was used for exact confirmation.Results: In 2019, the density of Amiota okadai in Foping National Nature Reserve, in China, increased sharply and infected Amiota okadai were newly found in wildlife home range. And it was newly found that wild giant pandas, wild boars, leopard cats, black bears were infected with Thelazia callipaeda. A total of 4 nematodes were collected. The morphologic characteristics of the nematode led to its identification as Thelazia callipaeda, which was molecularly confirmed by a specific PCR amplification.Conclusions: This is the first time in China that Amiota okadai has been reported to be infected with Thelazia callipaeda in wildlife home range, while a variety of wildlife, including wild giant pandas, have been infected. This suggests that there has been a transmission cycle of thelaziosis among wildlife in wildlife home range. This has undoubtedly increased the risk of infection of Thelazia callipaeda in villagers around wildlife home range.
Extraction And Quality Evaluation of Exosomes From Joint Effusion
Authors: Ruan Anmin , Chen Pu , Zhou Jun , Zhang Xiaozhe , Ma Yufeng , Wang Qingfu 
Published: July 2020

Abstract
Objective: The aim of this study was to compare the quality and efficiency of exosomes extracted from knee joint effusion by different methods, laying a foundation for further research on exosomes from knee joint effusion. Methods: Separate and extract joint exosomes by 8% polyethylene glycol (PEG) precipitation method, ultracentrifugation method (UC) and ultrafiltration with exclusion chromatography (SECF). Transmission electron microscopy (TEM) and nanoparticle tracing technology (NTA) were used to detect particle morphology and particle size, and Western Blot (WB) was used to detect granule protein surface marker proteins (CD9, CD63, Flotillin-1 and calnexin).Results: All three methods successfully separated vesicle-like round particles from joint effusion. TEM results show that the particles obtained by the three extraction methods are round or oval vesicles, the plasma membrane is intact, the size is different, and the diameter is distributed between 30 and 150 nm. Compared with SECF group, PEG group had more background particle impurities. and the broken vesicle fragments can be seen in the UC group; NTA results show that the main peaks of the three groups of particles are between 100-120nm, and the particle concentration is greater than 1×1010/ml; WB results show that the expressions of CD9, CD63 and Flotillin-1 protein in the suspension extracted by the three methods were all higher, the expression of calnexin protein was higher in the PEG group than the UC group and SECF group.Conclusion: The three extraction methods can successfully extract the exosomes of joint effusion. The quality of exosomes obtained by the SECF method is relatively high, while the PEG precipitation method contains a small amount of impurity particles. UC method does not guarantee the integrity of exosomes. In summary, when further studying the role of exosomes in the pathogenesis of knee osteoarthritis, we recommend the SECF method to extract and isolate joint exudates.
In-laboratory breast specimen radiography reduces tissue block utilization and improves turnaround time of pathologic examination
Authors: Arudra Sri Krishna Chaitanya , Garvey Laura C. , Hagemann Ian 
Published: July 2020

Abstract
Background
This study was performed to determine whether in-laboratory specimen radiography reduces turnaround time or block utilization in surgical pathology.
Methods
Specimens processed during a 48-day trial of an in-lab cabinet radiography device (Faxitron) were compared to a control group of specimens imaged in the mammography suite during a prior one-year period, and to a second group of specimens not undergoing imaging of any type.
Results
Cases imaged in the mammography suite had longer turnaround time than cases not requiring imaging (by 1.15 days for core biopsies, and 1.73 days for mastectomies; p < 0.0001). In contrast, cases imaged in-lab had turnaround time that was no longer than unimaged cases (p > 0.05 for core biopsies, lumpectomies and mastectomies). Mastectomies imaged in-lab required submission of fewer blocks than controls not undergoing any imaging (mean reduction of 10.6 blocks).
Conclusions
Availability of in-lab radiography resulted in clinically meaningful improvements in turnaround time and economically meaningful reductions in block utilization.
Analysis of Synchronization of Respiration-induced Motions in the Duodenum and Stomach for the Primary Tumor in Pancreatic Cancer Using Four-Dimensional Computed Tomography
Authors: Umezawa Rei , Wakita Akihisa , Katsuta Yoshiyuki , Ito Yoshinori , Nakamura Satoshi , Okamoto Hiroyuki , Kadoya Noriyuki , Takahashi Kana , Inaba Koji , Murakami Naoya , Igaki Hiroshi , Jingu Keiichi , Itami Jun 
Published: July 2020

Abstract
Background: We investigated the synchronization of respiration-induced motions at the primary tumor and organs at risk at radiation planning for pancreatic cancer.Methods: Four-dimensional (4D) computed tomography images were acquired under the condition of shallow free breathing in patients with pancreatic cancer. The gross tumor volume (GTV), duodenum (DU) and stomach (ST) were contoured. With reference to the 50% phase (exhale phase), excursions of respiration-induced motions of the GTV, DU and ST were measured. Based on the shift of the GTV, we investigated the synchronization of respiration-induced motions between each contouring target. We examined the differences in the overlap volumes between PTV and the planning organ at risk volume (PRV) in ST and DU and the differences in mean doses to the ST and DU in each respiratory phase.Results: Nine patients with pancreatic cancer were analyzed in this study. The mean maximum three-dimensional excursions at the GTV, DU and ST were 9.6, 9.8 and 11.4 mm, respectively. At phase 0% and 90% (inhale phases), mean distance changes in the positional relationship with the GTV were 0.3 and 0.7 mm, respectively, for the DU, and -2.5 and -2.4 mm, respectively for the ST, respectively. There was no significant difference in distance changes between each respiratory phase in the DU (p = 0.568), while there was a significant difference in distance changes in the ST (p < 0.001). There was a slight increase in the overlap volume between PTV and PRV in the expiratory phase in ST, but there were no significant differences between the respiratory phases either in ST or DU (p = 0.101 and 0.559). There was a significant difference in the change rates of mean doses in the respiratory phases in ST (p = 0.023), but there was no significant difference in DU (p = 0.933). Conclusions: Our results indicate that the DU but not the ST might move synchronously with GTV due to respiration.