bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel ultra-sensitive urine based FGFR3 mutation assay for diagnosis and recurrence detection of non-muscle-invasive bladder cancer (NMIBC) – Design of the Urodiag® Kit
Authors: ROPERCH Jean-Pierre , HENNION Claude 
Published: March 2020

Abstract
Background We have recently developed a highly accurate urine-based test, named Urodiag ® , associating FGFR3 mutation and DNA methylation assays for recurrence surveillance in patients with low-, intermediate-, and high-risk NMIBC. Previously, the detection of four FGFR3 mutations (G372C, R248C, S249C and Y375C) required the amplification steps and the PCR products were analyzed by capillary electrophoresis (Allele Specific-PCR, AS-PCR), which was expensive and time-consuming. Here, we present the development a novel ultra-sensitive multiplex PCR assay as called “Mutated Allele Specific Oligonucleotide-PCR (MASO-PCR)”, generating cost-effective, simple, fast and clinically applicable assay for the detection of FGFR3 mutations in voided urine. Methods Comparative clinical performances of MASO-PCR and AS-PCR technologies were performed from 263 urine DNA samples (87 FGFR3 mutated and 176 FGFR3 wild type). In the development of Urodiag ® Kit, we studied the stability and reproducibility of each all-in-one PCR master mix (single reaction mixture including all the necessary PCR components) for MASO-PCR and QM-MSPCR (Quantitative Multiplex Methylation-Specific PCR to co-amplify SEPTIN9, HS3ST2 and SLIT2 methylated genes) assays. Results Complete concordance (100%) was observed between the MASO-PCR and AS-PCR results. Each PCR master mix displayed excellent reproducibility and stability after 12 months of storage at -20°C, with intra-assay standard deviations lower than 0.3 Ct and coefficient of variations (CV) lower than 1%. The limit of detection (LoD) of MASO-PCR was 5% mutant detection in a 95% of wild-type background. The limit of quantification (LoQ) of QM-MSPCR was 10 pg of bisulfite-converted DNA. Conclusions We developed and clinically validated the MASO-PCR assay, generating cost-effective, simple, fast and clinically applicable assay for the detection of FGFR3 mutations in urine. We also designed the Urodiag ® Kit, which includes the MASO-PCR and QM-MSPCR assays. Adapted to routine clinical laboratory (simplicity, accuracy), the kit will be a great help to urologists for recurrence surveillance in patients at low-, intermediate- and high-risk NMIBC. Reducing the number of unnecessary cystoscopies, it will have extremely beneficial effects for patients (painless) and for the healthcare systems (low cost).
Development of A Novel Competing Risk Survival Predicting Model for Esophagogastric Junction Adenocarcinoma: a SEER population-based study
Authors: Wang Tongbo , Wu Yan , Zhou Hong , Wu Chaorui , Zhang Xiaojie , Chen Yingtai , Zhao Dongbing 
Published: March 2020

Abstract
Background: Adenocarcinoma in Esophagogastric Junction (AEG) is a severe gastrointestinal malignancy with a unique clinicopathological feature. To develop a competing risk nomogram for AEG patients and compared it with new 8th traditional tumor-node-metastasis (TNM) staging system.Methods: Based on AEG patients from the Surveillance, Epidemiology, and End Results (SEER) database between 2004 and 2010, we used the univariate and multivariate analysis to filter clinical factors and then built the competing risk nomogram to predicting the AEG cause-specific survival. We measured the clinical accuracy by comparing to the 8th TNM stage with receiver operating characteristic (ROC) curve, Brier score, and decision curve analysis (DCA).Results: Total of 1755 patients were included into this study. This nomogram was based on five variables: Number of examined lymph nodes, grade, invasion, metastatic LNs and age. The nomogram model was greater than traditional TNM staging with ROC curve (1-year AUC:0.747 vs. 0.641, 3-year AUC: 0.761 vs. 0.679, 5-year AUC: 0.759 vs. 0.682, 7-year AUC: 0.749 vs. 0.673, P<0.001), Brier score (3-year: 0.198 vs. 0.217, P=0.012; 5-year: 0.198 vs. 0.216, P=0.008; 7-year: 0.199 vs. 0.215, P=0.014) and DCA.Conclusions: Based on the SEER database with AEG patients, the competing risk nomogram showed the greater accurate individualized prediction of the survival compared with traditional TNM classification.
Risk factors of the suprasternal lymph node metastasis in papillary thyroid carcinoma with clinical lateral cervical lymph node metastasis
Authors: Kwon Hyun-Keun , Cheon Yong-Il , Shin Sung-Chan , Sung Eui-Suk , Lee Jin-Choon , Kim In-Ju , Lee Byung-Joo 
Published: March 2020

Abstract
Background: Metastatic lymph nodes are occasionally found in the suprasternal lymph nodes in patients with papillary thyroid cancer (PTC), but there are few studies on these lymph nodes. Therefore, we investigated the frequency and risk factors of suprasternal lymph node metastasis in PTC patients with lateral cervical lymph node metastasis.Methods: A total of 85 patients with cN1b PTC underwent total thyroidectomy with elective lateral neck dissection including the suprasternal lymph nodes. We analyzed the correlation between suprasternal lymph node metastasis and sex, age, tumor characteristics, and cervical lymph node metastasis status.Results: Eleven patients (12.9%) had pathological suprasternal lymph node metastasis. Suprasternal lymph node metastasis was associated with tumors located in the inferior pole and level IV lymph node metastasis.Conclusion: In cN1b PTC patients, especially those with inferior pole tumors and level IV nodal metastasis, the suprasternal lymph node should be routinely dissected.
Induced extracellular production of viniferins in grapevine cell culture medium by elicitation with methyl jasmonate and stevioside
Authors: Jeong Yu Jeong , Park Su Hyun , Park Sung-Chul , Kim Soyoung , Kim Tae Hee , Lee Jiyoung , Kim Suk Weon , Ryu Young Bae , Jeong Jae Cheol , Kim Cha Young 
Published: March 2020

Abstract
We report the high production of stilbenes, including resveratrol and viniferins, in grapevine (Vitis labruscana L.) cell cultures through elicitation with methyl jasmonate (MJ) and stevioside (STE). Methyl-β-cyclodextrin (CD-M) is widely used as a solubilizer for resveratrol production. For the first time, we used STE as a solubilizer for stilbene production in plant cell cultures. MJ was most effective in activating VvSTS expression and stimulating stilbene biosynthesis and induced accumulation of trans-piceid in the cells. High levels of trans-resveratrol and δ-viniferin production were observed in the culture medium when CD-M and STE, respectively, were used as solubilizers, but neither were significantly elevated within the cells. Maximum production of trans-resveratrol (12.2 mg/L) and δ-viniferin (892.2 mg/L) was observed 5 days after elicitation of cells with MJ and STE in shake flask cultures. Notably, predominant production of δ-viniferin and trans-resveratrol was observed in shake and static flask culture medium, respectively. Furthermore, stilbene compounds of resveratrol, e-viniferin, and δ-viniferin were mainly produced in a 3 L bioreactor culture following elicitation of cells with MJ and STE. These results provide new strategies for conditional, high-level production of resveratrol and viniferins in cell cultures by utilizing the solubilizing properties of STE or CD-M.
Identification of a gene from Streptomyces rimosus M527 negatively affecting antibiotic biosynthesis and morphological differentiation
Authors: Ma Zheng , Song Zhangqing , Xu Jie , Bechthold Andreas , Yu Xiaoping 
Published: March 2020

Abstract
Background: The polyene macrolide rimocidin, produced by Streptomyces rimosus M527, was found to be highly effective against a broad range of fungal plant pathogens. Current understanding of the regulatory mechanism of rimocidin biosynthesis and morphological differentiation in S. rimosus M527 is limited. NsdA is considered as a negative regulator involved in morphological differentiation and biosynthesis of secondary metabolites in some Streptomyces.Results: In this study, nsdAsr was cloned from S. rimosus M527. The role of nsdAsr in rimocidin biosynthesis and morphological differentiation was investigated by gene deletion, complementation, and over-expression. A ΔnsdAsr mutant was obtained using CRISPR/Cas9. The mutant produced more rimocidin (46%) and generated more spores than the wild-type strain. Over-expression of nsdAsr led to a decrease in rimocidin production and impairment of sporulation. Quantitative reverse transcription-PCR (qRT-PCR) analysis revealed that transcription of rim genes responsible for rimocidin biosynthesis was up-regulated in the ΔnsdAsr mutant but down-regulated in the nsdAsr over-expression strain. Similar effects have been described for Streptomyces coelicolor M145 and the industrial toyocamycin-producing strain Streptomyces diastatochromogenes 1628.Conclusion: NsdAsr is identified as a negative regulator of sporluation and antibiotic biosynthesis as well as the transcription of biosynthetic genes both in its host S. rimosus M527 and in model strain S. coelicolor and industrial producer strain S. diastatochromogenes 1628. This work will provide further information for understanding regulatory mechanisms controlling rimocidin biosynthesis in S. rimosus M527.
Knowledge, Attitude, Motivation and Planning of Breastfeeding; A cross Sectional Study among Jordanian Women.
Authors: khasawneh wasim , Kheirallah Khalid , Mazin Mai , Abdulnabi Sanaa 
Published: March 2020

Abstract
Background Antenatal women’s attitude and planning for breastfeeding are affected by their awareness and the support they receive. These are key factors that contribute to the trend variation in breastfeeding practice. This study aims to assess knowledge, attitude, support, and planning of breastfeeding among Jordanian women. Methods A face-to-face cross sectional semi-structured questionnaire survey was conducted among healthy women in the antenatal clinic and postpartum ward at three hospitals in Northern Jordan during the period August 2019 to December 2019. Data was collected about demographics, knowledge and attitude, support and counseling, and feeding planning. Factors associated with planning to breastfeed were reported. Results 660 women completed the survey questionnaires. 78% were knowledgeable about breastfeeding benefits and aware of WHO recommendations. 72% had a positive attitude towards breastfeeding. More than half received support from their husbands to breastfeed their infants, while less than 20% received any counseling from their obstetric physicians. 97% reported their intention to breastfeed, and more than half indicated their willing to exclusively breastfeed. With Multilogistic regression model, predictors of EBF planning include primiparty (AOR 1.79 95% C.I 1.1, 3.25), positive attitude (AOR 1.80 95% C.I 1.05, 3.1) and positive husband’s support (AOR 1.92 95% C.I. 1.18, 3.15). Barriers include women’s employment (AOR 0.43 95% C.I. 0.26, 0.70) and low birth weight (AOR 0.46 95% C.I. 0.25, 0.84) Conclusion Jordanian women are highly knowledgeable about breastfeeding benefits, and they exhibit a positive attitude towards breastfeeding resulting in a very high percentage intending to breastfeed their infants. Limited counseling about breastfeeding by obstetric physicians is a major gap in antenatal care. Assigning a specific time allotment for antenatal or postnatal counseling and support is expected to promote breastfeeding practice in our population.
SV40T reprograms Schwann cells into stem-like cells that can re-differentiate into terminal nerve cells.
Authors: Li Rui-Fang , Nan Guo-Xin , Wang Dan , Gao Chang , Yang Juan , He Tong-Chuan , Zhang Zhong-lin 
Published: March 2020

Abstract
Background : The effects of Simian virus 40 T antigen (SV40T) on various kinds of cells are different. Previous researchers failed to use SV40T immortalized nerve cells. However, they argued that SV40T caused nerve cell transformation. No one further study what is the specific effect of SV40T on nerve cells. We transfected Schwann cells (SCs) that did not have differentiation ability with MPH 86 plasmid containing SV40T in order to explore the effects of SV40T on Schwann cells. Methods : SCs were transfected with MPH 86 plasmid carrying the SV40T gene and cultured in different media, as well as co-cultured with neural stem cells (NSCs). In our study, SCs overexpressing SV40T were defined as SV40T-SCs. The proliferation of these cells was detected by WST-1, and the expression of different biomarkers was analyzed by qPCR and immunohistochemistry. Results: SV40T induced the characteristics of NSCs, such as the ability to grow in suspension, form spheroid colonies and proliferate rapidly, in the SCs, which were reversed by knocking out SV40T by the Flip-adenovirus . In addition, SV40T up-regulated the neurocrest markers Nestin, Pax3 and Slug, and down-regulated S100b as well as the late differentiation markers MBP, GFAP and Olig1/2. These cells also expressed NSC markers like Nestin, Sox2, CD133 and SSEA-1, as well as early development markers of embryonic stem cells (ESCs) like BMP4, c-Myc, OCT4 and Gbx2. Co-culturing with NSCs induced differentiation of the SV40T-SCs into neuronal and glial cells. Conclusions: SV40T reprograms Schwann cells to stem-like cells at the stage of neural crest cells that can differentiate to terminal nerve cells.
Different age pattern of COVID-19 cases in Hong Kong and Singapore by March 4, 2020
Authors: Zhu Shuying , Tao Jun , Gao Huizhi , He Daihai 
Published: March 2020

Abstract
Objective Coronavirus disease 2019 (COVID-19) now poses a huge threat to global public health and economics. It was found that elderly is more vulnerable to the infection. In this work, we compare the age structure of confirmed COVID-19 cases in Hong Kong SAR China and Singapore by March 4, 2020. Results We find significant difference in age patterns of confirmed cases in these two localities. We highlight the potential importance of population age structure in confirmed cases, which should be considered in evaluation of the effectiveness of control effort in different localities.
Does health reform achieve the goal of strengthening primary healthcare in China?
Authors: Feng Jie , Gong Yanhong , Li Hui , Wu Jianxiong , Zhang Jia , Lu Zuxun , Yin Xiaoxv 
Published: March 2020

Abstract
Background: Re-constructing the primary healthcare system is the focus of the new round of Chinese health reform. Nevertheless, there have been few studies focusing on whether the role of primary healthcare in the health system is strengthened in China.Methods: The data of this study came from the China Health Statistical Yearbook (2009–2018). We evaluated the development of primary healthcare based on the absolute values of health resources allocation and health service provision, and evaluated the status of primary healthcare throughout the health system based on the composition ratios of the indicators across the health system.Results: From 2009 to 2018, the amounts of health resources allocation and health service provision of Chinese primary healthcare institutions showed a significant upward trend (P<0.001). However, compared the indicators in 2009, excepting for the proportion of grants from the government in the whole health system has an upward trend, the proportions of other indicators had an escalating trend in 2018 by 4.04% for practicing (assistant) physicians, by 2.55% for nurses, by 4.06% for total revenues , by 5.54% for beds, by 7.37% for outpatient visits.Conclusion: The primary healthcare system has developed rapidly, but its development speed lagged behind the entire health system, resulting in the weakening of its actual functions, which is not in line with the goal of health reform. The government should improve the awareness of the importance of primary healthcare at all levels of local governments and ensure adequate financial input.
Willingness And Status Of Social Health Insurance Among People Of Pokhara Lekhnath, Nepal
Authors: Adhikari Manju , Gahatraj Nandram 
Published: March 2020

Abstract
Background: Social health insurance program was implemented in Nepal with the aim to achieve universal health coverage by removing health and financial barriers to access health care. This study was performed to assess the willingness and status of social health insurance among people of Pokhara Lekhnath, Nepal. Methods: A cross-sectional study was performed using mixed method. Quantitative study was conducted among 339 participants using interview schedule and qualitative among 27 participants using focus group discussion guidelines. Quantitative data was entered in Epi-data and descriptive analysis done in Statistical Package for Social Sciences. Chi-square tests was used for quantitative data to explore associations. Qualitative data was analyzed thematically keeping verbatim. Results: Among 339 participants, 51% were willing to pay and 77% were willing to continue, majority were due to the provision of free health services. About quarter enrolled participants were thinking about discontinuation due to lack of quality services. Presence of illness (0.003), age (<0.001), family-type (0.01), family-size (0.002), presence of chronic illness (0.002) and awareness level (<0.001) were significantly associated with enrollment to social health insurance program. Willingness to pay was determined by education (0.002) and awareness level (0.013). Maximum amount willingness to continue with is significantly associated with sex (0.037) Conclusion and Recommendations: Willingness to continue is higher than willingness to pay. Also, Awareness level is unsatisfactory. This suggests that people who knows about the social health insurance program get enrolled, learn and experience about the program and increase willingness to continue. Awareness was making significant influence over willingness to pay and enrollment. Lack of quality service was affecting willingness. Thus, the program should concerned on raising awareness and quality improvement.
Prognostic value of lactate levels and lactate clearance in sepsis and septic shock with initial hyperlactatemia: a retrospective cohort study according to the Sepsis-3 definitions
Authors: Lee Seong Geun , Song Juhyun , Cho Han-jin , Moon Sungwoo , Park Dae Won , Cha Jae Hyung 
Published: March 2020

Abstract
Background: This study evaluated the prognostic value of lactate levels and lactate clearance for the prediction of mortality in sepsis and septic shock patients.Methods: We performed a retrospective cohort study of sepsis and septic shock patients with initial lactate levels of ≥2 mmol/L. All patients met the Sepsis-3 definitions. The prognostic value of 6-hour lactate levels, lactate clearance, 6-hour lactate metrics (≥2 mmol/L), and lactate clearance metrics (<10%, <20%, and <30%) was evaluated. We compared the sensitivity and specificity between metrics.Results: Of the 363 sepsis and septic shock patients, 148 died (30-day mortality: 40.8%). Nonsurvivors had significantly higher 6-hour lactate levels and lower lactate clearance than those of survivors. Six-hour lactate levels and lactate clearance were associated with 30-day mortality after adjusting for potential confounders (odds ratio, 1.191 [95% confidence interval (CI), 1.097–1.294] and 0.989 [0.983–0.995], respectively). Six-hour lactate levels had better prognostic value than lactate clearance (area under the curve, 0.720 [95% CI, 0.670–0.765] vs. 0.656 [0.605–0.705]; p = 0.018). A 6-hour lactate level of ≥3.5 mmol/L and a lactate clearance of <24.4% were the optimal cutoff values in predicting the 30-day mortality. The prognostic value of 6-hour lactate metrics and lactate clearance metrics did not differ. Six-hour lactate levels (≥2 mmol/L) had the highest sensitivity (89.2%).Conclusions: Six-hour lactate levels proved to be more accurate in predicting 30-day mortality than lactate clearance.
Is all-inside with suspensory cortical button fixation a superior technique for anterior cruciate ligament reconstruction surgery? A systematic review and meta-analysis
Authors: Fu Chun Wei , Chen Wei-Cheng , Lu Yung-Chang 
Published: March 2020

Abstract
BackgroundTo compare the clinical results of all-inside anterior cruciate ligament reconstruction (ACLR) using suspensory cortical button fixation and full tibial tunnel drillingMethodsSystematic searches were conducted of published literature up to November 2019 on PubMed, Embase, and Cochrane for studies comparing all-inside ACLR using suspensory cortical button fixation and full tibial tunnel ACLR. Two reviewers independently determined eligibility, extracted the outcome data, and assessed the risk of bias of the eligible studies. The clinical outcome and graft reruptures were pooled by using random effects with mean differences and risk ratios for continuous and dichotomous variables, respectively.ResultA total of nine studies (five randomized controlled trials and four comparative studies) involving 613 patients were included in the meta-analysis. The postoperative functional outcome, knee laxity measured with arthrometer, and graft reruptures were comparable between patients with all-inside ACLR using suspensory cortical button fixation and full tibial tunnel ACLR. However, a significantly greater thickness of autologous tendon was used and less change in drilling tunnel diameter was noted in patients with suspensory cortical button graft fixation.ConclusionsAll-inside ACLR with suspensory cortical button fixation was not clinically superior to full tibial tunnel ACLR with interference screw fixation in functional outcomes, knee laxity measured with arthrometer, or rerupture rate. However, the advantage of using suspensory cortical button fixation was that a thicker graft could be used for reconstruction, and brought less tibia tunnel widening compared with bioabsorbable interference screw fixation.
Linkage between commuting time and sickness absence in the context of China: transmission channels and heterogeneous effects
Authors: Zicheng Wang , Jiachun Liu , Guo Murong 
Published: March 2020

Abstract
Background

Most of employees in urban China have experienced a heavy commuting burden,which has become an urgent issue that should be solved in the process of new urbanization strategy. However, not only has the exploration of relationship between the commuting and sickness absence been still scant in China, but also there is no discussion made to analyze the mechanism linking the commuting time and sickness absence. To address these gaps, this study firstly investigates the commuting-absence effect as well as the potential transmission channel between them.

Methods

Using a unique dataset of 2013 Matched Employer-Employee Survey (CMEES) in China, we apply the zero-inflated negative binomial model to explore the nexus between the commuting and sickness absence. To discuss the potential mechanism linking commuting and sickness absence in the context of China, the estimations of the commuting on health-related outcomes and work efforts are performed by the OLS and Logit regression to discuss the mechanism.

Results

The empirical results reveal that the commute has a positive effect on sickness absence, while it is still robust against several specifications. More importantly, the commuting-absence effect is mainly transmitted through health-related outcomes of employees, whereas we find no clear evidence supporting the shirking behaviors. Additionally, the heterogeneous effects of commuting-absence are differentiated across the Hukou status, gender, transportation modes, scale of cities and types of enterprises.

Conclusion

The longer commute induces to lower productivities through the sickness absence, that is, the longer journey from home to work is positively related with the increasing sickness absence, which keeps in consistency with previous studies. And the potential effect of commute-sickness absence is mainly transmitted through their health-related outcomes. Additionally, the impacts of commute time on sickness absence are differentiated across the Hukou status, gender, transportation modes, scale of cities and types of enterprise.