bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alterations of the paired maternal vaginal microbiome and neonatal meconium microbiome in vulvovaginal candidiasis positive pregnant women
Authors: Zhang Hongqin , Li Hongping , Zhang Ruolin , Ji Lingxia , Chen Jun , Nie Chuan , Huang Weimin 
Published: December 2024

BackgroundWomen with vulvovaginal candidiasis (VVC) are known to experience vaginal microbial dysbiosis. However, the dynamic alterations of the vaginal microbiome in pregnant women with VVC and its effect on neonatal gut microbiome remain unclear. This study aims to characterize the vaginal microbiome in pregnant women with VVC and its impact on their offspring’s meconium microbiome.MethodsForty-four pregnant women, including 17 with VVC (VVC group) and 27 healthy controls (HC group), along with their 44 offspring, were enrolled in this study. Maternal vaginal samples were collected during the pre- and post-delivery phases. Meconium samples from their newborns were also obtained. Microbial communities were characterized using 16S rRNA sequencing.ResultsThe vaginal microbiome of healthy pregnant women was predominantly composed of the genus Lactobacillus. The Bray-Curtis dissimilarity index indicated significant alterations in the vaginal microbiome of the VVC group, with a notable decrease in Lactobacillus and significant increases in Delftia, Burkholderia during both the pre- and post-delivery phases compared to the HC group. Additionally, the neonatal meconium microbiome exhibited significant differences between the VVC and HC groups, with L. salivarius and L. helveticus significantly decreased and Delftia significantly increased in the VVC group. Similar trends in microbial variation were observed across maternal and neonatal microbiomes, indicating intergenerational concordance associated with VVC.ConclusionVVC alters the microbiota of both pregnant women and their neonates at birth, suggesting a form of microbial inheritance. These findings underscore the distinctive characteristics of the vaginal microbiome associated with VVC and its potential impact on the formation of early-life gut microbiome.
Immunoinflammatory markers SIRI and NAR as predictors of respiratory distress syndrome and secondary infections in premature infants
Authors: Chen Xuexin , Li Yuedong , Chen Hongxiang , Chen Weiming 
Published: December 2024

BackgroundPremature infants are at high risk for neonatal respiratory distress syndrome (RDS) and secondary infections. This study aims to investigate the association between immunoinflammatory markers—the systemic immune inflammation index (SII), systemic inflammation response index (SIRI), and neutrophil-to-albumin ratio (NAR)—and the risk of developing RDS in premature infants.MethodsA total of 2164 premature infants were enrolled in this retrospective study. The clinical records of these neonates (respiratory tract infections, adverse pregnancy history, placental abnormalities, birth weight, Apgar scores, and immunoinflammatory indices) were collected. Comparisons were made between infants with and without RDS. Logistic regression analysis was used to evaluate the relationship between SII, SIRI, NAR and RDS.ResultsAmong the cohort, 962 infants developed RDS, while 1202 did not. The RDS group showed higher proportions of mothers with adverse pregnancy history, placental abnormalities, birth weight &lt;2.5 kg, and lower Apgar scores at 1 and 5 minutes (all p&lt;0.05). SII, SIRI, and NAR levels were significantly elevated in RDS infants (p&lt;0.05). Logistic regression revealed that adverse pregnancy history (OR: 1.390, p=0.001), placental abnormalities (OR: 2.499, p&lt;0.001), birth weight &lt;2.5 kg (OR: 4.165, p&lt;0.001), high SIRI (OR: 1.338, p=0.035), and high NAR (OR: 1.639, p&lt;0.001) were significant predictors of RDS. Additionally, secondary infections, particularly pneumonia and sepsis, were significantly more common in the RDS group (p&lt;0.001).ConclusionAdverse pregnancy history, placental abnormalities, low birth weight, elevated SIRI and NAR were associated with increased risk of RDS and secondary infections in premature infants. These findings suggest that SIRI and NAR could serve as useful markers for early identification and management of RDS and its complications in this vulnerable population.
The cellular and extracellular proteomic signature of human dopaminergic neurons carrying the LRRK2 G2019S mutation
Authors: Knab Felix , Guaitoli Giambattista , Jarboui Mohamed Ali , von Zweydorf Felix , Isik Fatma Busra , Klose Franziska , Rajkumar Anto Praveen , Gasser Thomas , Gloeckner Christian Johannes 
Published: December 2024

BackgroundExtracellular vesicles are easily accessible in various biofluids and allow the assessment of disease-related changes in the proteome. This has made them a promising target for biomarker studies, especially in the field of neurodegeneration where access to diseased tissue is very limited. Genetic variants in the LRRK2 gene have been linked to both familial and sporadic forms of Parkinson’s disease. With LRRK2 inhibitors entering clinical trials, there is an unmet need for biomarkers that reflect LRRK2-specific pathology and target engagement.MethodsIn this study, we used induced pluripotent stem cells derived from a patient with Parkinson’s disease carrying the LRRK2 G2019S mutation and an isogenic gene-corrected control to generate human dopaminergic neurons. We isolated extracellular vesicles and neuronal cell lysates and characterized their proteomic signature using data-independent acquisition proteomics. Then, we performed differential expression analysis to identify dysregulated proteins in the mutated line. We used Metascape and gene ontology enrichment analysis on the dysregulated proteomes to identify changes in associated functional networks.ResultsWe identified 595 significantly differentially regulated proteins in extracellular vesicles and 3,205 in cell lysates. We visualized functionally relevant protein–protein interaction networks and identified key regulators within the dysregulated proteomes. Using gene ontology, we found a close association with biological processes relevant to neurodegeneration and Parkinson’s disease. Finally, we focused on proteins that were dysregulated in both the extracellular and cellular proteomes. We provide a list of ten biomarker candidates that are functionally relevant to neurodegeneration and linked to LRRK2-associated pathology, for example, the sonic hedgehog signaling molecule, a protein that has tightly been linked to LRRK2-related disruption of cilia function.ConclusionIn conclusion, we characterized the cellular and extracellular proteome of dopaminergic neurons carrying the LRRK2 G2019S mutation and proposed an experimentally based list of biomarker candidates for future studies.
Development and validation of a predicative model for identifying sarcopenia in Chinese adults using nutrition indicators (AHLC)
Authors: Zhao Xin , Yan Pengdong , Chen Ningxin , Han Tingting , Wang Bin , Hu Yaomin 
Published: December 2024

BackgroundSarcopenia, a condition characterized by low muscle mass, plays a critical role in the health of older adults. Early identification of individuals at risk is essential to prevent sarcopenia-related complications. This study aimed to develop a predictive model using readily available clinical nutrition indicators to facilitate early detection.MethodsA total of 1,002 participants were categorized into two groups: 819 with normal skeletal muscle mass (SMM) and 183 with low muscle mass (sarcopenia). A predictive model was developed for sarcopenia risk via multivariate logistic regression, and its performance was assessed using four analyses: receiver operating characteristic (ROC) curve analysis, decision curve analysis (DCA), a nomogram chart, and external validation. These methods were used to evaluate the model’s discriminative ability and clinical applicability.ResultsIn the low-SMM group, more females (55.73% vs. 40.42%) and older individuals (median 61 vs. 55 years) were observed. These patients had lower albumin (41.00 vs. 42.50 g/L) and lymphocyte levels (1.60 vs. 2.02 × 109/L) but higher HDL (1.45 vs. 1.16 mmol/L) and calcium levels (2.24 vs. 2.20 mmol/L) (all p &lt; 0.001). Using LASSO regression, we developed a nutritional AHLC (albumin + HDL cholesterol + lymphocytes + calcium) model for sarcopenia risk prediction. AUROC and DCA analyses, as well as nomogram charts and external validation, confirmed the robustness and clinical relevance of the AHLC model for predicting sarcopenia.ConclusionOur study employs serum nutrition indicators to aid clinicians in promoting healthier aging. The AHLC model stands out for weight-independent evaluations. This novel approach could assess sarcopenia risk in the Chinese population, thereby enhancing aging and quality of life.
Carbon reserves in coffee agroforestry in the Peruvian Amazon
Authors: Vallejos-Torres Geomar , Gaona-Jimenez Nery , Pichis-García Roger , Ordoñez Luis , García-Gonzales Patricia , Quinteros Aníbal , Lozano Andi , Saavedra-Ramírez Jorge , Tuesta-Hidalgo Juan C. , Reategui Keneth , Macedo-Córdova Wilder , Baselly-Villanueva Juan R. , Marín César 
Published: December 2024

IntroductionSecondary forests and coffee cultivation systems with shade trees might have great potential for carbon sequestration as a means of climate change adaptation and mitigation. This study aimed to measure carbon stocks in coffee plantations under different managements and secondary forest systems in the Peruvian Amazon rainforest (San Martín Region).MethodsThe carbon stock in secondary forest trees was estimated using allometric equations, while carbon stocks in soil, herbaceous biomass, and leaf litter were determined through sampling and laboratory analysis.ResultsThe biomass carbon stock in secondary forests was 132.2 t/ha, while in coffee plantations with Inga sp. shade trees was 118.2 t/ha. Carbon stocks were 76.5 t/ha in coffee with polyculture farming, while the lowest amount of carbon was found in coffee without shade trees (31.1 t/ha). The carbon sequestered by coffee plants in all agroforestry systems examined had an average of 2.65 t/ha, corresponding to 4.63 % of the total carbon sequestered, being the highest stored in the coffee system with Inga sp. shade trees. A higher content of glomalin-related soil proteins (GRSP) was found in coffee without shade trees, with 18.5 mg/g.DiscussionThese results point to Inga sp. as a compatible model of shade system for coffee farms. However, broader-scale time-average measurements and carbon dioxide emissions should be assessed in these study systems to have a full understanding of their climate impacts.
Internet- and mobile-based aftercare and relapse prevention interventions for anxiety and depressive disorders: a systematic review
Authors: Petre Ligiana Mihaela , Piepiora Paweł Adam , Gemescu Maria , Gheorghe Delia Alexandra 
Published: December 2024

BackgroundDigital interventions present potential solutions for aftercare and relapse prevention in anxiety and depressive disorders. This systematic review synthesizes evidence on the efficacy of internet- and mobile-based interventions for post-acute care in these conditions.MethodsA systematic search was conducted in electronic databases (MEDLINE, CENTRAL, Scopus, Web of Science, PsycINFO, PsycARTICLES, PsycEXTRA, ProQuest Dissertations and Theses Open, Open Access Theses and Dissertations, and Open Grey) for randomized controlled trials evaluating digital aftercare or relapse prevention interventions for adults with anxiety or depressive disorders. Primary outcomes included symptom severity, relapse rates, recurrence rates, and rehospitalization. Secondary outcomes included general quality of life and adherence to primary treatment. Risk of bias was assessed using the Cochrane tool.ResultsNineteen studies (3,206 participants) met the inclusion criteria. Interventions included cognitive-behavioral therapy, mindfulness-based approaches, and supportive text messaging. Most studies focused on depression, with limited evidence for anxiety disorders. Notably, fourteen studies that reported on depressive symptoms demonstrated significant improvements following digital interventions, with effect sizes ranging from small (Cohen’s d = 0.20) to large (Cohen’s d = 0.80). Five studies investigated relapse or recurrence rates, yielding mixed results. Adherence rates varied significantly across studies, ranging from 50 to 92.3%, highlighting the variability in participant engagement. Methodological quality was also variable, with allocation concealment and blinding being common limitations.ConclusionInternet- and mobile-based interventions show promise for aftercare and relapse prevention in depression, with limited evidence for anxiety disorders. Future research should focus on optimizing engagement, personalizing interventions, standardizing outcome measures, and conducting larger trials with longer follow-up periods. These findings have important implications for integrating digital tools into existing care pathways to improve long-term outcomes for individuals with anxiety and depressive disorders.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020151336, CRD42020151336.
Psychometric evaluation of the abbreviated Hungarian Faking Orgasm Scale for Women
Authors: Csányi Edit , Basler Julia , Bereczkei Tamás , Meskó Norbert 
Published: December 2024

IntroductionThe Faking Orgasm Scale for Women (FOS) was developed to explore the motivations behind women’s self-reported instances of faking orgasm during oral sex and sexual intercourse. In a recent study, a Hungarian version of the FOS was developed, confirming the same factor structure as the original American version, consisting of four factors across two subscales.MethodsThe current study aimed to develop and validate a brief Hungarian FOS. Factor analysis was conducted with data from 2220 women (mean age = 24.4, SD = 7.48 years). The Item Response Theory (IRT) analysis indicated that retaining four-four scales, each comprising of three items was the optimal solution for the revised shorter version. Validation involved 768 women (mean age = 22.6, SD = 4.54 years) completing a questionnaire package, including the Hungarian Short Form of Reasons for Having Sex Questionnaire (YSEX?-HSF) and Women’s Sexual Working Models Scale (WSWMS).ResultsThe results suggest that the Hungarian 24-item FOS, with its four scales within each of the two sub-scales, provides a reliable and valid measurement of motives for faking orgasm in women. The different reasons behind faking orgasm are associated with different sexual working patterns and sexual motivations. Furthermore, women who reported faking orgasms reported significantly higher levels of sexual distancing and perceived lower care from their partners compared to women who reported not faking orgasms. Women who reported faking orgasm were also more likely to engage in sexual activities to attain personal goals and cope with emotional stress.DiscussionThe FOS-24 offers both practitioners and researchers a concise and useful instrument for the assessment of faking orgasms.
Prevalence of falls and associations with family functioning among community-dwelling older adults in Guangzhou, China
Authors: Sun Si-Yu , Wang Zhi-Wei , Peng Zhi-Li , Yuan Le-Xin , Yang Wen-Hao , Zhang Wen-Jian , Chen Jia-Min , Lin Wei-Quan , Zhu Wei 
Published: December 2024

IntroductionFalls are the primary cause of unintentional fatalities among individuals aged 65 and older. Enhancing research on fall prevention among older adults is an urgent priority. Consequently, this study aims to investigate the prevalence and influencing factors of falls among community-dwelling older adults in Guangzhou, China, with a particular emphasis on the impact of family functioning.MethodsWe used a multi-stage stratified cluster random sampling technique to successfully survey 2,399 individuals aged 65 and above across 11 districts in Guangzhou City. Data on sociodemographic characteristics, health and lifestyle factors, and fall incidents were collected through telephone interviews. Chi-square tests, Lasso regression, and logistic regression were utilized to pinpoint fall risk factors. Association rule mining uncovered the relationships between falls and associated variables.ResultsA total of 390 participants reported experiencing falls, the prevalence of falls among older adults was 16.3% (95%CI: 14.82% ~ 17.78%). Logistic regression analysis identified several risk factors for falls among older adults: female [OR = 1.511, 95%CI (1.188–1.922)], age 85 years and older [OR = 2.332, 95%CI (1.447–3.758)], stroke [OR = 1.821, 95%CI (1.038–3.192)], hypoglycemia [OR = 1.639, 95%CI (1.228–2.186)], visual impairment [OR = 1.418, 95%CI (1.097–1.833)], need to be cared for [OR = 1.722, 95%CI (1.339–2.215)], chronic pain [OR = 1.663, 95%CI (1.302–2.124)], and anxiety [OR = 1.725, 95%CI (1.243–2.395)]. In addition, it was shown that a well-functioning family was a protective factor against falls [OR = 0.589, 95%CI (0.44–0.789)].ConclusionThe prevalence of falls among community-dwelling older adults in Guangzhou City was high, and the influencing factors were complex. It is recommended to develop and implement comprehensive intervention measures for high-risk groups, including those who are females, older adults, and suffer from chronic diseases while paying special attention to the care of family members for older adults.
Fumagillin Shortage: How to Treat Enterocytozoon bieneusi Microsporidiosis in Solid Organ Transplant Recipients in 2024?
Authors: Garrouste Cyril , Poirier Philippe , Uro-Coste Charlotte , Iriart Xavier , Kamar Nassim , Bonhomme Julie , Calvar Eve , Le Gal Solène , Lanfranco Luca , Autier Brice , Rakoff Lucien , Durieux Marie-Fleur , Danthu Clément , Morio Florent , Deltombe Clément , Moreno-Sabater Alicia , Ouali Nacera , Costa Damien , Bertrand Dominique , Chesnay Adélaïde , Gatault Philippe , Rabodonirina Meja , Morelon Emmanuel , Dumortier Jérôme , Sitterlé Emilie , Scemla Anne , Hamane Samia , Cachera Laurène , Damiani Céline , Poulain Coralie , L’Ollivier Coralie , Moal Valérie , Delhaes Laurence , Kaminski Hannah , Cateau Estelle , Ecotière Laure , Brunet Julie , Caillard Sophie , Valot Stéphane , Tinel Claire , Argy Nicolas , Raimbourg Quentin , Robert Marie Gladys , Noble Johan , Boignard Aude , Botterel Françoise , Matignon Marie , Bellanger Anne-Pauline , Crépin Thomas , Leroy Jordan , Lionet Arnaud , Debourgogne Anne , Nicolas Muriel , Claudéon Joëlle , Moniot Maxime , Lambert Céline , Nourrisson Céline 
Published: December 2024

Intestinal microsporidiosis caused by Enterocytozoon bieneusi is an opportunistic infection that especially affects solid organ transplant (SOT) recipients. Management revolves around tapering the immunosuppressive regimen and/or using a specific anti-microsporidia treatment, but only fumagillin has demonstrated efficacy for treatment of this infection. Since fumagillin has been commercially discontinued, nitazoxanide is increasingly being used in this indication. We aimed to describe therapeutic management of E. bieneusi infections in this context. We conducted a French nationwide observational retrospective study on reported cases of E. bieneusi infections in SOT recipients. We identified 154 cases: 64 (41.6%) were managed by simply modifying the immunosuppressive regimen, 54 (35.1%) were given fumagillin, and 36 (23.4%) were given nitazoxanide. Clinical remission rate ranged from 77.8% to 90.7% and was not significantly different between therapeutic strategies but tended to be lower with nitazoxanide. Stool negativization rate was highest with fumagillin (91.7%) and lowest with nitazoxanide (28.6%). Relapses occurred in 6.9% of cases and were more frequent with nitazoxanide (14.3%). This study shows that tapering immunosuppression can result in a satisfactory remission rate but is sometimes accompanied by relapses. Nitazoxanide had limited effectiveness, whereas fumagillin had good results that provide a solid rationale for bringing fumagillin back to market.Trial Registration NumberClinicalTrials.gov ID: NCT05417815.
Modified Ji-Huan He’s Frequency Formulation for Large-Amplitude Electron Plasma Oscillations
Authors: Kontomaris Stylianos Vasileios , Malamou Anna , Psychogios Ioannis , Ismail Gamal M. 
Published: December 2024

This paper examines oscillations governed by the generic nonlinear differential equation u″=ωp021−u∓2β2uγ, where ωp0, β and γ are positive constants. The aforementioned differential equation is of particular importance, as it describes electron plasma oscillations influenced by temperature effects and large oscillation amplitudes. Since no analytical solution exists for the oscillation period in terms of ωp0, β,γ and the oscillation amplitude, accurate approximations are derived. A modified He’s approach is used to account for the non-symmetrical oscillation around the equilibrium position. The motion is divided into two parts: umin≤u<ueq and ueq<u≤umax, where umin and umax are the minimum and maximum values of u, and ueq is its equilibrium value. The time intervals for each part are calculated and summed to find the oscillation period. The proposed method shows remarkable accuracy compared to numerical results. The most significant result of this paper is that He’s approach can be readily extended to strongly non-symmetrical nonlinear oscillations. It is also demonstrated that the same approach can be extended to any case where each segment of the function f(u) in the differential equation u″+fu=0 (for umin≤u<ueq and for ueq<u≤umax) can be approximated by a fifth-degree polynomial containing only odd powers.
Нутритивная и респираторная поддержка при боковом амиотрофическом склерозе
Authors: Рушкевич Ю.Н. , Труханова И.С. , Мальгина Е.В. , Золотухина М.В. , Гвищ Т.Г. 
Published: December 2024

Статья посвящена нутритивной и респираторной поддержке пациентов с боковым амиотрофическим склерозом. Нутритивные и дыхательные нарушения относятся к самым частым осложнениям бокового амиотрофического склероза. Стратегия лечения пациентов с боковым амиотрофическим склерозом направлена на поддержание питания и респираторной функции, что позволяет замедлить прогрессирование болезни. Нутритивная поддержка пациентов с боковым амиотрофическим склерозом с соблюдением высококалорийной диеты и дробного питания помогает снизить скорость потери массы тела. Пациентам с умеренной или выраженной дисфагией необходимо устанавливать гастростому. Пациентам с признаками дыхательной недостаточности следует назначать неинвазивную вентиляцию легких, в более сложных случаях рассматривать вопрос об искусственной вентиляции легких. Важными аспектами являются регулярный мониторинг респираторных и нутритивных показателей и индивидуализированный подход, адаптированный к стадии заболевания. Все методы по нутритивной и респираторной поддержке пациентов с боковым амиотрофическим склерозом направлены на продление и улучшение качества жизни.
The article is dedicated to nutritional and respiratory support for patients with amyotrophic lateral sclerosis. Nutritional and respiratory disorders are among the most frequent complications of amyotrophic lateral sclerosis. The treatment strategy for patients with amyotrophic lateral sclerosis is focused on maintaining nutrition and respiratory function to slow the disease progression. The nutritional support for patients with amyotrophic lateral sclerosis with high-calorie diet and split meals helps to reduce the rate of body weight loss. Patients with moderate to severe dysphagia should be fitted by a gastrostomy. Patients with signs of respiratory failure should be prescribed non-invasive ventilation, and in more complicated cases artificial ventilation should be considered. A regular monitoring of respiratory and nutritional indicators and an individualized approach adapted to the disease stage are essential. All methods of nutritional and respiratory support for patients with amyotrophic lateral sclerosis are intended for prolonging and improving the quality of life.