bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response of St. Augustinegrass cultivars to pinoxaden alone and in combination with select fungicides
Authors: Peppers John M. , Harris James , McElroy J. Scott 
Published: March 2020

AbstractPinoxaden is a herbicide inhibiting acetyl coenzyme A carboxylase recently introduced for use in turfgrass. Currently, pinoxaden is labeled for use in all bermudagrass [Cynodon dactylon (L.) Pers.] and zoysiagrass (Zoysia spp.) turfgrass areas except putting greens. However, pinoxaden is only labeled for use in St. Augustinegrass [Stenotaphrum secundatum (Walter) Kuntze] sod production because of injury concerns. Tank mixtures of two or more herbicides can reduce injury to turfgrass species. Injury reduction can also occur when fungicides are tank‐mixed with herbicides. Research was conducted between 2018 and 2019 to determine the effect that pinoxaden alone and tank mixtures of pinoxaden with one of two common fungicides, chlorothalonil and propiconazole, would have on four different cultivars of St. Augustinegrass: ‘Classic’, ‘Floratam’, ‘Palmetto’, and ‘Raleigh’. At the conclusion of the study, we determined that there is differential susceptibility to pinoxaden between cultivars of St. Augustinegrass and that both chlorothalonil and propiconazole have a safening effect on pinoxaden applications to St Augustinegrass, regardless of cultivar. The cultivars Classic and Raleigh were more susceptible to pinoxaden than Floratam and Palmetto. All four cultivars were less susceptible to pinoxaden when chlorothalonil and propiconazole were applied in combination with pinoxaden. These results suggest that pinoxaden can be applied in a tank mixture with either chlorothalonil or propiconazole to reduce pinoxaden injury to St. Augustinegrass. However, the effect that these tank mixtures may have on weed control is currently unknown.
Molecular Interrogation to Crack the Case of O‐GlcNAc
Authors: Estevez Arielis , Zhu Dongsheng , Blankenship Connor , Jiang Jiaoyang 
Published: March 2020

AbstractThe O‐linked β‐N‐acetylglucosamine (O‐GlcNAc) modification, termed O‐GlcNAcylation, is an essential and dynamic post‐translational modification in cells. O‐GlcNAc transferase (OGT) installs this modification on serine and threonine residues, whereas O‐GlcNAcase (OGA) hydrolyzes it. O‐GlcNAc modifications are found on thousands of intracellular proteins involved in diverse biological processes. Dysregulation of O‐GlcNAcylation and O‐GlcNAc cycling enzymes has been detected in many diseases, including cancer, diabetes, cardiovascular and neurodegenerative diseases. Here, recent advances in the development of molecular tools to investigate OGT and OGA functions and substrate recognition are discussed. New chemical approaches to study O‐GlcNAc dynamics and its potential roles in the immune system are also highlighted. It is hoped that this minireview will encourage more research in these areas to advance the understanding of O‐GlcNAc in biology and diseases.
Widely Electronically Tunable 2,6‐Disubstituted Dithieno[1,4]thiazines—Electron‐Rich Fluorophores Up to Intense NIR Emission
Authors: May Lars , Müller Thomas J. J. 
Published: March 2020

Abstract2,6‐Difunctionalized dithieno[1,4]thiazines were efficiently synthesized by (pseudo)five‐ or (pseudo)three‐component one‐pot processes based on lithiation‐electrophilic trapping sequences. As supported by structure–property relationships, the thiophene anellation mode predominantly controls the photophysical and electrochemical properties and the electronic structures (as obtained by DFT calculations). From molecular geometries and redox potentials to fluorescence quantum yields in solution, the interaction of the dithieno[1,4]thiazine‐core with the substituents causes striking differences within the series of regioisomers. Most interestingly, strong acceptors introduced in anti–anti dithieno[1,4]thiazines nearly induce a planarization of the ground‐state geometry and a highly intense NIR fluorescence (ΦF=0.52), whereas an equally substituted syn–syn dithieno[1,4]thiazine exhibits a much stronger folded molecular structure and fluoresces poorly (ΦF=0.01). In essence, electrochemical and photophysical properties of dithieno[1,4]thiazines can be tuned widely and outscore the compared phenothiazine with cathodically shifted oxidation potentials and redshifted and more intense absorption bands.
Active Metal Template Synthesis and Characterization of a Nanohoop [c2]Daisy Chain Rotaxane
Authors: Van Raden Jeff M. , Jarenwattananon Nanette N. , Zakharov Lev N. , Jasti Ramesh 
Published: March 2020

AbstractMolecules and materials that demonstrate large amplitude responses to minor changes in their local environment play an important role in the development of new forms of nanotechnology. Molecular daisy chains are a type of a mechanically interlocked molecule that are particularly sensitive to such changes in which, in the presence of certain stimuli, the molecular linkage enables muscle‐like movement between a reduced‐length contracted form and an increased‐length expanded form. To date, all reported syntheses of molecular daisy chains are accomplished via passive‐template methods, resulting in a majority of structures being switchable only through the addition of an exogenous stimuli such as metal ions or changes in pH. Here, we describe a new approach to these structural motifs that exploits a multi‐component active‐metal template synthesis to mechanically interlock two pi‐rich nanohoop macrocycles into a molecular daisy chain that undergoes large conformational changes using thermal energy.
Supervised Learning of Gene Regulatory Networks
Authors: Razaghi‐Moghadam Zahra , Nikoloski Zoran 
Published: March 2020

AbstractIdentifying the entirety of gene regulatory interactions in a biological system offers the possibility to determine the key molecular factors that affect important traits on the level of cells, tissues, and whole organisms. Despite the development of experimental approaches and technologies for identification of direct binding of transcription factors (TFs) to promoter regions of downstream target genes, computational approaches that utilize large compendia of transcriptomics data are still the predominant methods used to predict direct downstream targets of TFs, and thus reconstruct genome‐wide gene‐regulatory networks (GRNs). These approaches can broadly be categorized into unsupervised and supervised, based on whether data about known, experimentally verified gene‐regulatory interactions are used in the process of reconstructing the underlying GRN. Here, we first describe the generic steps of supervised approaches for GRN reconstruction, since they have been recently shown to result in improved accuracy of the resulting networks? We also illustrate how they can be used with data from model organisms to obtain more accurate prediction of gene regulatory interactions. © 2020 The Authors.Basic Protocol 1: Construction of features used in supervised learning of gene regulatory interactionsBasic Protocol 2: Learning the non‐interacting TF‐gene pairsBasic Protocol 3: Learning a classifier for gene regulatory interactions
A novel mathematical teletraffic method to evaluate the interoperation of different standards with NOMA systems using order statistics
Authors: Borja‐Benítez Miguel A. , Vasquez‐Toledo Luis A. , Tirado‐Méndez José A. , Borja‐Benítez Berenice , Marcelin‐Jiménez Ricardo 
Published: March 2020

SummaryBasically, nonorthogonal multiple access (NOMA) allows controllable interferences by nonorthogonal resource allocation with a tolerable increase in receiver complexity, using successive interference cancellation (SIC). In this paper, authors show a novel mathematical model of teletraffic for users accessing, simultaneously, to Wi‐Fi and LTE services by using NOMA, calculating the birth–death process by order statistics. This methodology has not been previously used. The mathematical model is represented by a two‐dimensional Markovian process where the users can born or die in one system or another, independently, and results are validated through simulations. The outcomes demonstrate an increment of the sum‐rate gain for a system where users are ordered in the power domain using order statistics, according to the signal interference plus noise ratio (SINR), compared to a system where these statistics are not employed. By using the proposed mathematical model, the sum‐rate gain increases around 15%, and it is also demonstrated that this algorithm foresees a steady behavior of the sum rate, even when the number of channels is augmented, contrary when no order statistics are employed, in which the sum rate decreases considerably. On the other hand, the blocking probability is also demonstrated based on the chance of moving from one technology to another.
Continuous capture of recombinant antibodies by ZnCl2 precipitation without polyethylene glycol
Authors: Dutra Gregory , Komuczki Daniel , Jungbauer Alois , Satzer Peter 
Published: March 2020

AbstractThe capture of recombinant antibodies from cell culture broth is the first critical step of downstream processing. We were able to develop a precipitation‐based method for the capture and purification of monoclonal antibodies based on divalent cations, namely ZnCl2. Traditional precipitation processes have to deal with high dilution factors especially for resolubilization and higher viscosity due to the use of PEG as precipitation or co‐precipitation agent. By the use of the crosslinking nature of divalent cations without the use of PEG, we kept viscosity from the supernatant and resolubilization dilution factors very low. This is especially beneficial for the solid–liquid separation for the harvest and wash of the precipitate in continuous mode. For this harvest and wash, we used tangential flow filtration that benefits a lot from low viscosity solutions, which minimizes the membrane fouling. With this precipitation based on ZnCl2, we were able to implement a very lean and efficient process. We demonstrated precipitation studies with three different antibodies, Adalimumab, Trastuzumab, and Denosumab, and a continuous capture case study using tangential flow filtration for precipitate recovery. In this study, we achieved yields of 70%.
Multisite survey of bacterial contamination in ready‐to‐eat meat products throughout the cooking and selling processes in urban supermarket, Nanjing, China
Authors: Wang Shao‐Kang , Fu Ling‐Meng , Chen Guo‐Wei , Xiao Hong‐Mei , Pan Da , Shi Ruo‐Fu , Yang Li‐Gang , Sun Gui‐Ju 
Published: March 2020

AbstractObjectiveReady‐to‐eat (RTE) meat is a kind of popular instant food easily contaminated by microbes, which is one of the causes of foodborne diseases. This study analyzes the possible sources of RTE food bacterial contamination during processing and subsequent selling.MethodSamples of eight kinds of RTE meat were collected from four supermarkets in Nanjing, China. The knives, chopping boards, trays(containers of food), clamps, air, water, and hands of the sales staff were sampled, and the enumeration of aerobic plate count and total coliforms and pathogenic bacteria was performed.ResultsThe survey revealed that poor hygienic levels was the causes that RTE meat products were contaminated by bacteria at different levels. With regard to pathogen, the incidences of Salmonella spp. and Staphylococcus aureus were 4.2% and 2.1%, respectively. These results also revealed that the bacterial contamination of RTE food was caused by the air, as well as clamps, chopping boards, knives, trays, and hands of the operators. The total number of aerobic colonies were positively correlated with the amount of RTE food in one pot (r = .87728, p = .0217), and negatively correlated with the maximum temperature in the center of the meat (r = −.81633, p = .0475).ConclusionThe high number of bacteria in RTE foods indicates potential food safety risks and the need to improve the health of supermarket sales staff. The most important thing is to determine how to raise hygiene awareness of employees through food safety education. Meanwhile, a comprehensive set of regulations on hand cleaning and disinfection should be developed to facilitate public health and reduce foodborne illness caused by the consumption of RTE food.
Validation of PAS Kinase, a Regulator of Hepatic Fatty Acid and Triglyceride Synthesis, as a Therapeutic Target for Nonalcoholic Steatohepatitis
Authors: Swiatek Wojciech , Parnell K. Mark , Nickols G. Allen , Scharschmidt Bruce F. , Rutter Jared 
Published: March 2020

Hyperactivation of sterol regulatory element binding protein 1c (SREBP‐1c), which transcriptionally induces expression of enzymes responsible for de novo lipogenesis and triglyceride (TG) formation, is implicated in nonalcoholic fatty liver disease (NAFLD)/nonalcoholic steatohepatitis (NASH) pathogenesis. Posttranslational SREBP‐1c maturation and activation is stimulated by the protein per–arnt–sim kinase (PASK). PASK‐knockout mice are phenotypically normal on a conventional diet but exhibit decreased hypertriglyceridemia, insulin resistance, and hepatic steatosis on a high‐fat diet. We investigated the effects of pharmacologic PASK inhibition using BioE‐1115, a selective and potent oral PASK inhibitor, in Zucker fatty (fa)/fa) rats, a genetic model of obesity, dyslipidemia, and insulin resistance, and in a dietary murine model of NAFLD/NASH. Female Zucker (fa/fa) rats and lean littermate (fa/+) controls received BioE‐1115 (3‐100 mg/kg/day) and/or omega‐3 fatty acids, and blood glucose, hemoglobin A1c, glucose tolerance, insulin, and serum TG were measured. C57BL/6J mice fed a high‐fat/high‐fructose diet (HF‐HFrD) were treated with BioE‐1115 (100 mg/kg/day) or vehicle. Body weight and fasting glucose were measured regularly; serum TG, body and organ weights, and liver TG and histology were assessed at sacrifice. Messenger RNA (mRNA) abundance of SREBP‐1c target genes was measured in both models. In Zucker rats, BioE‐1115 treatment produced significant dose‐dependent reductions in blood glucose, insulin, and TG (all greater than omega‐3 fatty acids) and dose dependently restored insulin sensitivity assessed by glucose tolerance testing. In HF‐HFrD mice, BioE‐1115 reduced body weight, liver weight, fasting blood glucose, serum TGs, hepatic TG, hepatic fibrosis, hepatocyte vacuolization, and bile duct hyperplasia. BioE‐1115 reduced SREBP‐1c target mRNA transcripts in both models. Conclusion: PASK inhibition mitigates many adverse metabolic consequences associated with an HF‐HFrD and reduces hepatic fat content and fibrosis. This suggests that inhibition of PASK is an attractive therapeutic strategy for NAFLD/NASH treatment.
Developing novel in silico prediction models for assessing chemical reproductive toxicity using the naïve Bayes classifier method
Authors: Zhang Hui , Shen Chen , Liu Ru‐Zhuo , Mao Jun , Liu Chun‐Tao , Mu Bo 
Published: March 2020

AbstractAssessment of reproductive toxicity is one of the important safety considerations in drug development. Thus, in the present research, the naïve Bayes (NB)‐classifier method was applied to develop binary classification models. Six important molecular descriptors for reproductive toxicity were selected by the genetic algorithm. Then, 110 classification models were developed using six molecular descriptors and10 types of fingerprints with 11 different maximum diameters. Among these established models, the model based on six molecular descriptors and the SciTegic extended‐connectivity fingerprints with 20 maximum diameters (LCFC_20) displayed the best prediction performance for reproductive toxicity (NB‐1), which gave a 0.884 receiver operating characteristic (ROC) score and 91.8% overall prediction accuracy for the Training Set, and produced a 0.888 ROC score and 83.0% overall accuracy for the external Test Set I. In addition, for the external rat multi‐generation reproductive toxicity dataset (Test Set II), the NB‐1 model generated a 0.806 ROC score and 85.1% concordance. The generated prediction results indicated that the NB‐1 model could give robust and reliable predictions for a reproductive toxicity potential of chemicals. Thus, the established model could be applied to filter early‐stage molecules for potential reproductive adverse effects. In addition, six important molecular descriptors and new structural alerts for reproductive toxicity were identified, which could help medicinal chemists rationally guide the optimization of lead compounds and select chemicals with the best prospects of being safe and effective.
Serial section Raman tomography with 10 times higher depth resolution than confocal Raman microscopy
Authors: Böhm Thomas , Moroni Riko , Thiele Simon 
Published: March 2020

AbstractConfocal Raman microscopy enables 3D imaging of various samples solely based on chemical contrast. However, optical artifacts impair resolution and image quality in subsurface imaging. With serial section Raman tomography, we show that serial ultrathin and semithin sectioning by ultramicrotomy can successfully be combined with subsequent confocal Raman imaging. This new 3D Raman imaging technique reaches a depth resolution of up to 100 nm, which is about 10‐fold better than in confocal Raman microscopy. Structurally complex and optically inhomogeneous samples can not only be imaged, but also be used to quantify structural parameters. Serial section Raman tomography is a promising method for materials science and possibly also for life sciences.
Modeling of Rhamnolipid Biosurfactant Production: Estimation of Kinetic Parameters by Genetic Algorithm
Authors: Câmara Jéssica M.D.A. , Sousa Magna A.S.B. , Barros Neto Eduardo L. 
Published: March 2020

AbstractStudies about kinetics and modeling of production parameters for biosurfactants are essential to the development of efficient processes from an economic point of view. In this sense, this work evaluated the performance of four nonstructured models to explain the experimental data for biomass growth, substrate consumption, and rhamnolipid production using glycerol as carbon source and a Pseudomonas aeruginosa strain. The kinetic parameters of each model were estimated using a global search method known as genetic algorithm and numerical discretization of differential equations by the Runge–Kutta 4th order method. The main result of this study showed that the Monod model best represented the experimental data, with μmax values of 0.06 h−1, KS of 50.8 g L−1, YX/S of 0.43 g g−1, and YP/X equal to 0.017 g g−1.
Vagus nerve stimulation paired with tones for tinnitus suppression: Effects on voice and hearing
Authors: Kochilas Helen L. , Cacace Anthony T. , Arnold Amy , Seidman Michael D. , Tarver W. Brent 
Published: March 2020

AbstractObjectiveIn individuals with chronic tinnitus, our interest was to determine whether daily low‐level electrical stimulation of the vagus nerve paired with tones (paired‐VNSt) for tinnitus suppression had any adverse effects on motor‐speech production and physiological acoustics of sustained vowels. Similarly, we were also interested in evaluating for changes in pure‐tone thresholds, word‐recognition performance, and minimum‐masking levels. Both voice and hearing functions were measured repeatedly over a period of 1 year.Study designLongitudinal with repeated‐measures.MethodsDigitized samples of sustained frontal, midline, and back vowels (/e/, /o/, /ah/) were analyzed with computer software to quantify the degree of jitter, shimmer, and harmonic‐to‐noise ratio contained in these waveforms. Pure‐tone thresholds, monosyllabic word‐recognition performance, and MMLs were also evaluated for VNS alterations. Linear‐regression analysis was the benchmark statistic used to document change over time in voice and hearing status from a baseline condition.ResultsMost of the regression functions for the vocal samples and audiometric variables had slope values that were not significantly different from zero. Four of the nine vocal functions showed a significant improvement over time, whereas three of the pure tone regression functions at 2‐4 kHz showed some degree of decline; all changes observed were for the left ear, all were at adjacent frequencies, and all were ipsilateral to the side of VNS. However, mean pure‐tone threshold changes did not exceed 4.29 dB from baseline and therefore, would not be considered clinically significant. In some individuals, larger threshold shifts were observed. No significant regression/slope effects were observed for word‐recognition or MMLs.ConclusionQuantitative voice analysis and assessment of audiometric variables showed minimal if any evidence of adverse effects using paired‐VNSt over a treatment period of 1 year. Therefore, we conclude that paired‐VNSt is a safe tool for tinnitus abatement in humans without significant side effects.Level of evidenceLevel IV.
The torsion‐free part of the Ziegler spectrum of orders over Dedekind domains
Authors: Gregory Lorna , L'Innocente Sonia , Toffalori Carlo 
Published: March 2020

AbstractWe study theR‐torsion‐free part of the Ziegler spectrum of an order Λ over a Dedekind domainR. We underline and comment on the role of lattices over Λ. We describe the torsion‐free part of the spectrum when Λ is of finite lattice representation type.
Variations in gut bacterial communities between lesser white‐fronted geese wintering at Caizi and Shengjin lakes in China
Authors: Liu Gang , Gong Zhizhong , Li Qingyue 
Published: March 2020

AbstractThe avian gut microbiota plays an important role in shaping the health of its host. However, knowledge of gut bacteria in birds lags behind that of other animals. In this study, we investigated the gut bacterial communities of lesser white‐fronted geese (Anser erythropus) wintering at Shengjin Lake and Caizi Lake, China, using high‐throughput sequencing (Illumina MiSeq). Altogether, 1,053,624 high‐quality sequences and 4,405 operational taxonomic units (OTUs) were acquired from 30 fecal samples (15 per lake). The OTUs represented eight phyla and 17 classes from the Caizi Lake samples and seven phyla and 16 classes from the Shengjin Lake samples. Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were the dominant phyla. The spatial distance and the Chao1, Simpson, and Shannon indices showed that the alpha diversity differed significantly between the samples from both lakes. The phylogenetic tree and heatmap analyses showed that all the Caizi Lake samples were clustered together and all the Shengjin Lake samples were clustered together. These findings suggest that diet may be an important driver of gut microbial community structure in the birds from each lake, and the obvious differentiation in their gut microbial structures may indicate that the bacteria are highly sensitive to food sources at both lakes.
Regionales Fonds‐Stipendiatentreffen in München
Authors: Bruckmoser Jonas 
Published: March 2020

AbstractMitte Dezember 2019 trafen sich im bayerischen Raum ansässige Stipendiaten des Fonds der Chemischen Industrie (FCI) an der Technischen Universität München (TUM). Die Veranstaltung hatten Bernhard Rieger, Professor für makromolekulare Chemie, und sein Arbeitskreis organisiert. Die 13 Nachwuchswissenschaftler, die durch ein Liebig‐ oder Kekulé‐Stipendium des FCI gefördert werden, tauschten sich aus und gaben in kurzen Vorträgen Einblicke in ihre Arbeitsprojekte.