bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adhesion between P. falciparum infected erythrocytes and human endothelial receptors follows alternative binding dynamics under flow and febrile conditions
Authors: Lubiana Pedro , Bouws Philip , Roth Lisa Katharina , Dörpinghaus Michael , Rehn Torben , Brehmer Jana , Wichers Jan Stephan , Bachmann Anna , Höhn Katharina , Roeder Thomas , Thye Thorsten , Gutsmann Thomas , Burmester Thorsten , Bruchhaus Iris , Metwally Nahla Galal 
Published: March 2020

AbstractCharacterizing the adhesive dynamics of Plasmodium falciparum infected erythrocytes (IEs) to different endothelial cell receptors (ECRs) in flow is a big challenge considering available methods. This study investigated the adhesive dynamics of IEs to five ECRs (CD36, ICAM-1, P-selectin, CD9, CSA) using simulations of in vivo-like flow and febrile conditions. To characterize the interactions between ECRs and knobby and knobless IEs of two laboratory-adapted P. falciplarum isolates, cytoadhesion analysis over time was performed using a new tracking bioinformatics method. The results revealed that IEs performed rolling adhesion exclusively over CD36, but exhibited stationary binding to the other four ECRs. The absence of knobs affected rolling adhesion both with respect to the distance travelled by IEs and their velocity. Knobs played a critical role at febrile temperatures by stabilizing the binding interaction. Our results clearly underline the complexity of the IE-receptor interaction and the importance of knobs for the survival of the parasite at fever temperatures, and lead us to propose a new hypothesis that could open up new strategies for the treatment of malaria.
Downregulation of histone methyltransferase SET8 inhibits progression of hepatocellular carcinoma
Authors: Wu Jianhua , Qiao Kuangyuan , Du Yanming , Zhang Xiaoyun , Cheng Haichao , Peng Li , Guo Zhanjun 
Published: March 2020

AbstractThe expression of lysine methyltransferase SET8, which is involved in carcinogenesis of many types of human cancers through monomethylation of histone H4 lysine 20 (H4K20), is associated with the prognosis of hepatocellular carcinoma (HCC). We performed a functional analysis for SET8 to assess its effect on HCC progression. SET8 knockdown inhibited proliferation, migration and invasion of HCC cells. SET8 knockdown also inhibited tumour growth in a human xenograft mouse model. Overexpression of SET8 displayed the reverse effect, while treatment with the SET8 inhibitor UNC0379 produced an effect similar to SET8 knockdown. In addition, drug sensitivity testing in SET8-siRNA transfected HCC cells indicated that docetaxel inhibited cell growth dramatically, as demonstrated by the Cell Counting Kit-8 (CCK-8) assay. Furthermore, gene expression microarray analysis showed that genes altered after SET8 knockdown were clustered in pathways related to tumorigenesis and metastasis. Our data suggests that targeting SET8 for HCC therapy can inhibit the proliferation and invasion of HCC cells as well as increase their sensitivity to chemotherapy.
Nintedanib ameliorates animal model of dermatitis
Authors: Heo Min-Jeong , Lee Chanmi , Choi Soo Young , Choi Yeong Min , An In-sook , Bae Seunghee , An Sungkwan , Jung Jin Hyuk 
Published: March 2020

AbstractNintedanib, a receptor tyrosine kinase (RTK) inhibitor has been developed as therapeutics for idiopathic pulmonary fibrosis and non-small lung cancer. We found that the expression levels of RTK, especially VEGFR1 is increased in skin biopsies of dermatitis patients from multiple independent datasets. Moreover, VEGFR1 is highly expressed by infiltrated cells in dermis from oxazolone (OXA) treated mice. Interestingly, nintedanib alleviates dermatitis symptom in OXA-induced animal model. Especially, levels of epidermis thickness, infiltrated immune cells including mast cells and eosinophils were decreased from mice cotreated with nintedanib and OXA compared with OXA treated mice. Moreover, serum IgE and Th2 cytokines including IL-4 and IL-13 were decreased by nintedanib treatment. These results suggest an evidence that nintedanib alleviates animal model of dermatitis.
Phonatory and articulatory representations of speech production in cortical and subcortical fMRI responses
Authors: Correia Joao M. , Caballero-Gaudes César , Guediche Sara , Carreiras Manuel 
Published: March 2020

AbstractSpeaking involves coordination of multiple neuromotor systems, including respiration, phonation and articulation. Developing non-invasive imaging methods to study how the brain controls these systems is critical for understanding the neurobiology of speech production. Recent models and animal research suggest that regions beyond the primary motor cortex (M1) help orchestrate the neuromotor control needed for speaking, including cortical and sub-cortical regions. Using contrasts between speech conditions with controlled respiratory behavior, this fMRI study investigates articulatory gestures involving the tongue, lips and velum (i.e., alveolars versus bilabials, and nasals versus orals), and phonatory gestures (i.e., voiced versus whispered speech). Multivariate pattern analysis (MVPA) was used to decode articulatory gestures in M1, cerebellum and basal ganglia. Furthermore, apart from confirming the role of a mid-M1 region for phonation, we found that a dorsal M1 region, linked to respiratory control, showed significant differences for voiced compared to whispered speech despite matched lung volume observations. This region was also functionally connected to tongue and lip M1 seed regions, underlying its importance in the coordination of speech. Our study confirms and extends current knowledge regarding the neural mechanisms underlying neuromotor speech control, which hold promise to study neural dysfunctions involved in motor-speech disorders non-invasively.
Super impact stable TATB explosives recrystallized by bicarbonate ionic liquids with a record solubility
Authors: Yuan Wen-Li , Tao Guo-Hong , Zhang Lei , Zhang Zhang , Xue Ying , He Ling , Huang Jinglun , Yu Weifei 
Published: March 2020

AbstractEnsuring the security for long-term storage of weapons is always of the great cMehilaloncerns in the field of energetic materials. 1,3,5-Triamino-2,4,6-trinitrobenzene (TATB) is a remarkable explosive applied in nuclear weapons where extreme safety is required primarily. Owing to the strong inter or intra molecular hydrogen bonding, TATB shows poor solubility in most solvents. As the result, the particle shape and size of TATB products is hard to regulate, which closely related to the weapons stability. Herein, a new recrystallization method is provided to refine TATB using bicarbonate ionic liquids. Bicarbonate ionic liquids exhibited the record solubility (26.7 wt%) for dissolving TATB explosive. The recrystallized TATB were spherical particles with uniform size and showed extremely insensitivity to impact (>100 J) and friction (>360 N). Moreover, the experimental1H and13C NMR spectra of TATB in solution are reported for the first time.
Aureobasidium pullulans volatilome identified by a novel, quantitative approach employing SPME-GC-MS, suppressed Botrytis cinerea and Alternaria alternata in vitro
Authors: Yalage Don S. M. , Schmidtke L. M. , Gambetta J. M. , Steel C. C. 
Published: March 2020

AbstractVolatile organic compounds (VOCs) produced by Aureobasidium pullulans were investigated for antagonistic actions against Alternaria alternata and Botrytis cinerea. Conidia germination and colony growth of these two phytopathogens were suppressed by A. pullulans VOCs. A novel experimental setup was devised to directly extract VOCs using solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) from antagonist-pathogen culture headspace. The proposed system is a robust method to quantify microbial VOCs using an internal standard. Multivariate curve resolution-alternating least squares deconvolution of SPME-GC-MS spectra identified fourteen A. pullulans VOCs. 3-Methyl-1-hexanol, acetone, 2-heptanone, ethyl butyrate, 3-methylbutyl acetate and 2-methylpropyl acetate were newly identified in A. pullulans headspace. Partial least squares discriminant analysis models with variable importance in projection and selectivity ratio identified four VOCs (ethanol, 2-methyl-1-propanol, 3-methyl-1-butanol and 2-phenylethanol), with high explanatory power for discrimination between A. pullulans and pathogen. The antifungal activity and synergistic interactions of the four VOCs were evaluated using a Box-Behnken design with response surface modelling. Ethanol and 2-phenylethanol are the key inhibitory A. pullulans VOCs against both B. cinerea and A. alternata. Our findings introduce a novel, robust, quantitative approach for microbial VOCs analyses and give insights into the potential use of A. pullulans VOCs to control B. cinerea and A. alternata.
Electrochemical Degradation of Reactive Black 5 using two-different reactor configuration
Authors: Droguett Tamara , Mora-Gómez Julia , García-Gabaldón Montserrat , Ortega Emma , Mestre Sergio , Cifuentes Gerardo , Pérez-Herranz Valentín 
Published: March 2020

AbstractNovel Sb-doped SnO2 ceramic electrodes sintered at different temperatures, are applied to the degradation of Reactive Black 5 in both divided and undivided electrochemical reactors. In the undivided reactor the discoloration of the solution took place via the oxidation of RB5 dye, without the corresponding reduction in the chemical oxygen demand for the ceramic electrodes. However, in the divided one, it was possible to achieve the discoloration of the solution while at the same time decreasing the chemical oxygen demand through the ·OH-mediated oxidation, although the chemical oxygen demand degradation took place at a slower rate.
In vivo engineering of lymphocytes after systemic exosome-associated AAV delivery
Authors: Breuer Cort B. , Hanlon Killian S. , Natasan Jeya-shree , Volak Adrienn , Meliani Amine , Mingozzi Federico , Kleinstiver Benjamin P. , Moon James J. , Maguire Casey A. 
Published: March 2020

AbstractEx-vivo gene therapy using stem cells or T cells transduced by retroviral or lentiviral vectors has shown remarkable efficacy in the treatment of immunodeficiencies and cancer. However, the process is expensive, technically challenging, and not readily scalable to large patient populations, particularly in underdeveloped parts of the world. Direct in vivo gene therapy would avoid these issues, and such approaches with adeno-associated virus (AAV) vectors have been shown to be safe and efficacious in clinical trials for diseases affecting differentiated tissues such as the liver and CNS. However, the ability to transduce lymphocytes with AAV in vivo after systemic delivery has not been carefully explored. Here, we show that both standard and exosome-associated preparations of AAV8 vectors can effectively transduce a variety of immune cell populations including CD4+ T cells, CD8+ T cells, B cells, macrophages, and dendritic cells after systemic delivery in mice. We provide direct evidence of T cell transduction through the detection of AAV genomes and transgene mRNA, and show that intracellular and transmembrane proteins can be expressed. These findings establish the feasibility of AAV-mediated in vivo gene delivery to immune cells which will facilitate both basic and applied research towards the goal of direct in vivo gene immunotherapies.
Constraining the masses of microlensing black holes and the mass gap with Gaia DR2
Authors: Wyrzykowski Łukasz , Mandel Ilya 
Published: March 2020

Context. Gravitational microlensing is sensitive to compact-object lenses in the Milky Way, including white dwarfs, neutron stars, or black holes, and could potentially probe a wide range of stellar-remnant masses. However, the mass of the lens can be determined only in very limited cases, due to missing information on both source and lens distances and their proper motions.
Aims. Our aim is to improve the mass estimates in the annual parallax microlensing events found in the eight years of OGLE-III observations towards the Galactic Bulge with the use of Gaia Data Release 2 (DR2).
Methods. We use Gaia DR2 data on distances and proper motions of non-blended sources and recompute the masses of lenses in parallax events. We also identify new events in that sample which are likely to have dark lenses; the total number of such events is now 18.
Results. The derived distribution of masses of dark lenses is consistent with a continuous distribution of stellar-remnant masses. A mass gap between neutron star and black hole masses in the range between 2 and 5 solar masses is not favoured by our data, unless black holes receive natal kicks above 20−80 km s−1. We present eight candidates for objects with masses within the putative mass gap, including a spectacular multi-peak parallax event with mass of 2.4−1.3+1.9 M⊙ located just at 600 pc. The absence of an observational mass gap between neutron stars and black holes, or conversely the evidence of black hole natal kicks if a mass gap is assumed, can inform future supernova modelling efforts.
Asymmetrical nebula of the M33 variable GR290 (WR/LBV)
Authors: Maryeva Olga V. , Koenigsberger Gloria , Karpov Sergey V. , Lozinskaya Tatiana A. , Egorov Oleg V. , Rossi Corinne , Calabresi Massimo , Viotti Roberto F. 
Published: March 2020

Context. GR 290 (M 33 V0532 = Romano’s star) is a luminous M 33 object undergoing photometric variability typical for luminous blue variable stars. It lies inside the Wolf-Rayet region in the Hertzsprung-Russell diagram and possesses a WN8-type spectrum at the light minima. Analysis of Gran Telescopio Canarias spectra obtained in 2016 led to the conclusion that it is surrounded by an unresolved H II region formed mostly of ejected material from the central star and revealed the presence of a second, more extended asymmetrical emission region.
Aims. The aim of this paper is to further explore the structure of the nearby environment of GR 290.
Methods. Long-slit spectra of GR 290 were obtained with three slit orientations in the visual and red spectral regions. The emission-line distribution for each slit was analyzed.
Results. We confirm the presence of an asymmetric H II region that extends ~50 pc to the south; ~30 pc to the north, and southeast; ~20 pc to the east and northwest; and ~10 pc to the west. We also present the first spectrum to be acquired of a star belonging to the neighboring OB 88 association, J013501.87+304157.3, which we classify as a B-type supergiant with a possible binary companion.
Phase mixing and wave heating in a complex coronal plasma
Authors: Howson T. A. , De Moortel I. , Reid J. 
Published: March 2020

Aims. We investigate the formation of small scales and the related dissipation of magnetohydronamic (MHD) wave energy through non-linear interactions of counter-propagating, phase-mixed Alfvénic waves in a complex magnetic field.
Methods. We conducted fully three-dimensional, non-ideal MHD simulations of transverse waves in complex magnetic field configurations. Continuous wave drivers were imposed on the foot points of magnetic field lines and the system was evolved for several Alfvén travel times. Phase-mixed waves were allowed to reflect off the upper boundary and the interactions between the resultant counter-streaming wave packets were analysed.
Results. The complex nature of the background magnetic field encourages the development of phase mixing throughout the numerical domain, leading to a growth in alternating currents and vorticities. Counter-propagating phase-mixed MHD wave modes induce a cascade of energy to small scales and result in more efficient wave energy dissipation. This effect is enhanced in simulations with more complex background fields. High-frequency drivers excite localised field line resonances and produce efficient wave heating. However, this relies on the formation of large amplitude oscillations on resonant field lines. Drivers with smaller frequencies than the fundamental frequencies of field lines are not able to excite resonances and thus do not inject sufficient Poynting flux to power coronal heating. Even in the case of high-frequency oscillations, the rate of dissipation is likely too slow to balance coronal energy losses, even within the quiet Sun.
Conclusions. For the case of the generalised phase-mixing presented here, complex background field structures enhance the rate of wave energy dissipation. However, it remains difficult for realistic wave drivers to inject sufficient Poynting flux to heat the corona. Indeed, significant heating only occurs in cases which exhibit oscillation amplitudes that are much larger than those currently observed in the solar atmosphere.
An elastic model of Phobos’ libration
Authors: Yang Yongzhang , Yan Jianguo , Guo Xi , He Qingbao , Barriot Jean-Pierre 
Published: March 2020

Context. Study the rotation of a celestial body is an efficient way to infer its interior structure, and then may give information of its origin and evolution. In this study, based on the latest shape model of Phobos from Mars Express (MEX) mission, the polyhedron approximation approach was used to simulate the gravity field of Phobos. Then, the gravity information was combined with the newest geophysical parameters such as GM and k2 to construct the numerical model of Phobos’ rotation. And with an appropriate angles transformation, we got the librational series respect to Martian mean equator of date.
Aims. The purpose of this paper is to develop a numerical model of Phobos’ rotational motion that includes the elastic properties of Phobos. The frequencies analysis of the librational angles calculated from the numerical integration results emphasize the relationship between geophysical properties and dynamics of Phobos. This work will also be useful for a future space mission dedicated to Phobos.
Methods. Based on the latest shape model of Phobos from MEX mission, we firstly modeled the gravity field of Phobos, then the gravity coefficients were combined with some of the newest geophysical parameters to simulate the rotational motion of Phobos. To investigate how the elastic properties of Phobos affect its librational motion, we adopted various k2 into our numerical integration. Then the analysis was performed by iterating a frequency analysis and linear least-squares fit of Phobos’ physical librations. From this analysis, we identified the influence of k2 on the largest librational amplitude and its phase.
Results. We showed the first ten periods of the librational angles and found that they agree well with the previous numerical results which Phobos was treated as a perfectly rigid body. We also found that the maximum amplitudes of the three parameters of libration are also close to the results from a rigid model, which is mainly due to the inclination of Phobos and moments of inertia. The other amplitudes are slightly different, since the physics contained in our model is different to that of a previous study, specifically, the different low-degree gravity coefficients and ephemeris. The libration in longitude τ has the same quadratic term with previous numerical study, which is consistent with the secular acceleration of Phobos falling onto Mars. We investigated the influence of the tidal Love number k2 on Phobos’ rotation and found a detectable amplitude changes (0.0005°) expected in the future space mission on τ, which provided a potential possibility to constrain the k2 of Phobos by observing its rotation. We also studied the influence of Phobos’ orbit accuracy on its libration and suggested a simultaneous integration of orbit and rotation in future work.
The Use of Zirconium and Feldspathic Porcelain in the Management of the Severely Worn Dentition: A Case Report
Authors: Malkoc Meral Arslan , Sevimay Mujde , Yaprak Emre 
Published: March 2020

ABSTRACTThe management of the interim phase of a complete oral rehabilitation in patients with severely worn dentition is often challenging due to the loss of occlusal vertical dimension, loss of tooth structure, uneven wear of teeth creating an uneven plane of occlusion, and para-functional habits. This case report describes the management of excessive tooth tissue loss in a 45 year old woman with a history of bruxism, esthetical complaints in anterior teeth, and impaired dental function due to reduced tooth height. The patient used occlusal splint for a month and than resection of the alveolar bone was performed on the vestibular sides of the maxillary anterior teeth, except the interdental alveolar crest. Maxillary anterior teeth were restored with zirconia porcelain. Feldspathic porcelain was chosen to restore remaining teeth in both jaws; the patient also was given an occlusion guard to protect the restoration against future bruxism. Regardless of the cause of occlusal instability, it is important that the restorative dentist should be able to recognize its signs such as tooth hypermobility, tooth wear, periodontal breakdown, occlusal dimpling, stress fractures, exostosis, muscle enlargement, and loss of posterior disclusion. When restoring the worn dentition, the clinician should bear in mind the five P’s: proper planning prevents poor performance. (Eur J Dent 2009;3:75-80)
Interdisciplinary Treatment of a Fused Lower Premolar with Supernumerary Tooth
Authors: Gadimli Cengiz , Sari Zafer 
Published: March 2020

ABSTRACTThe objective of this report is to describe combined orthodontic and endodontic treatment of a fused mandibular premolar with supernumerary tooth. The patient was a 15 year old girl seeking orthodontic treatment for the correction of maxillary and mandibular crowding. Cephalometric examination revealed skeletally Class I relationship. The panoramic radiograph showed a fused tooth with two separate pulp chambers and two separate root canals connecting in apical third. After the endodontic treatment of the fused teeth, the stripping of the supernumerary tooth was performed to establish a Class I canine relationship and to correct midline deviation. At the end of the treatment, the crowding was resolved and positive overjet and overbite was achieved. (Eur J Dent 2011;5:349-353)
Platelets and Fibrinogen: Emerging Complexity in Trauma-Induced Coagulopathy
Authors: John Alexander E. , White Nathan J. 
Published: March 2020

AbstractTrauma induces a change in nearly every observable aspect of hemostasis, generally tipping the balance toward trauma-induced coagulopathy (TIC) and bleeding in the critical early stages. Two particularly important aspects of TIC are platelets and fibrinogen, which are the primary determinants of clot formation and hemostasis. Their loss and dysfunction represent important transition points between coagulopathy phenotypes, highlighting their mechanistic roles in TIC as well as unveiling new potential avenues toward important diagnostic and therapeutic interventions. This review synthesizes current knowledge of platelets and fibrinogen during TIC, with a focus on emerging concepts related to their dysfunction and development of new therapeutic approaches.
Forward to the past
Authors: LaPolla Randy J. 
Published: March 2020

AbstractThis paper argues that linguistic typology, and linguistics more generally, got off to a good start in the 19th century with scholars like Wilhelm von Humboldt and Georg von der Gabelentz, where the understanding was that each language manifests a unique world view, and it is important to study and compare those world views. This tradition is still alive, but was sidelined and even denigrated for many years due to the rise of Structuralism, which attempted to study language structures divorced from their linguistic and socio-cultural contexts. The paper reviews the understandings the early scholars had and points out their similarities with cutting edge current views in cognitive linguistics, construction grammar, and interactional linguistics, which had to be rediscovered due to the influence of Structuralism for so many years. It then argues that we should make linguistic typology (and linguistics more generally) more modern, scientific, and empirical by returning to our roots.