bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acceptance of Robot‐Mediated Teaching and Therapy for Children With Atypical Development by Polish Professionals
Authors: Kossewska Joanna , Kłosowska Joanna 
Published: January 2020

AbstractTechnological innovations have resulted in the production of new, super‐high‐tech assistive robotic devices that improve the development of children with atypical development. The theoretical basis for this study was the unified theory of acceptance and use of technology (UTAUT) model, which consists of four main constructs that directly determine user acceptance and user behavior: performance expectancy, effort expectancy, social influence, and facilitating conditions. The purpose of the study was to understand the rationale behind the acceptance of robot‐mediated teaching and therapy for children with atypical development by Polish professionals. Participants included 245 professionals recruited from the staff of special centers and schools for children with disabilities in the south of Poland. They were first presented with a video demonstration of the humanoid Nao robot and then given a 31‐item questionnaire that estimates UTAUT. Participants' attitudes towards using the Nao robot in teaching and therapy for children with atypical development were positive. However, these attitudes were found to be a weak albeit significant predictor of behavioral intention to use the Nao device. They were significantly predicted by social influence, effort expectancy, and performance expectancy. The results are discussed in the context of the general theoretical background and the latest findings related to professionals working with disabled children. Correlation and structural modeling analyses confirmed the applicability of the UTAUT model in education and care for children with disabilities. However, behavioral intention was weakly related to positive attitudes towards Nao robot in this Polish sample. Forthcoming questions follow the conclusions of the current study.
Host and symbiont genetic determinants of nutritional phenotype in a natural population of the pea aphid
Authors: Chung Seung Ho , Parker Benjamin J. , Blow Frances , Brisson Jennifer A. , Douglas Angela E. 
Published: January 2020

AbstractA defining feature of the nutritional ecology of plant sap‐feeding insects is that the dietary deficit of essential amino acids (EAAs) in plant sap is supplemented by EAA‐provisioning microbial symbionts in the insect. Here, we demonstrated substantial variation in the nutritional phenotype of 208 genotypes of the pea aphid Acyrthosiphon pisum collected from a natural population. Specifically, the genotypes varied in performance (larval growth rates) on four test diets lacking the EAAs arginine, histidine and methionine or aromatic EAAs (phenylalanine and tryptophan), relative to the diet containing all EAAs. These data indicate that EAA supply from the symbiotic bacteria Buchnera can meet total aphid nutritional demand for only a subset of the EAA/aphid genotype combinations. We then correlated single nucleotide polymorphisms (SNPs) identified in the aphid and Buchnera genomes by reduced genome sequencing against aphid performance for each EAA deletion diet. This yielded significant associations between performance on the histidine‐free diet and Buchnera SNPs, including metabolism genes predicted to influence histidine biosynthesis. Aphid genetic correlates of performance were obtained for all four deletion diets, with associations on the arginine‐free diet and aromatic‐free diets dominated by genes functioning in the regulation of metabolic and cellular processes. The specific aphid genes associated with performance on different EAA deletion diets are largely nonoverlapping, indicating some independence in the regulatory circuits determining aphid phenotype for the different EAAs. This study demonstrates how variation in the phenotype of associations collected from natural populations can be applied to elucidate the genetic basis of ecologically important traits in systems intractable to traditional forward/reverse genetic techniques.
Identification of the targeted therapeutic potential of doxycycline for a subset of gastric cancer patients
Authors: Pandian Jaishree , Panneerpandian Ponmathi , Devanandan Helen Jemimah , Sekar Balaji T. , Balakrishnan Karthik , Selvarasu Karthikeyan , Muthupandi Karthikeyan , Ganesan Kumaresan 
Published: January 2020

AbstractThe identification of new drugs for the targeted therapy of gastric cancer remains an important need. The RAS/RAF/MEK/ERK/ELK1 signaling cascade is activated in many cancers, including gastric cancer. To identify the targetable inhibitors of the ERK/MAPK pathway, we performed a repurposing screening of a panel of antimicrobial agents in gastric cancer cells using an ERK/MAPK‐driven firefly luciferase reporter assay. Multiple antibiotics were identified to inhibit ERK‐mediated transcriptional activity. Among them, doxycycline showed high inhibition of ERK/MAPK‐regulated transcriptional activity and the levels of ERK proteins. Doxycycline was further identified to inhibit the proliferation and the colony‐ and spheroid‐forming potential of gastric cancer cells. By in vitro signaling pathway and genome‐wide expression profiling analyses, doxycycline was identified to inhibit signaling pathways and transcriptional activities regulated by ER, Myc, E2F1, Wnt, SMAD2/3/4, Notch, and OCT4. Doxycycline was also found to activate p53‐, ATF6‐, NRF1/2‐, and MTF1‐mediated transcription and inhibit the transcription of histones, proteasomal genes, fibroblast growth factor, and other oncogenic factors. These observations show the multitargeting and targeted therapeutic features of doxycycline for a subset of gastric tumors.
Laboratory‐based interventions targeting food craving: A systematic review and meta‐analysis
Authors: Wolz Ines , Nannt Julia , Svaldi Jennifer 
Published: January 2020

SummaryThis systematic review and meta‐analysis aimed to quantify the effects of laboratory‐based interventions targeting specific mechanisms of food craving, to identify moderators of effects, and to qualitatively summarize findings. The study was conducted and reported according to the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines. Sixty‐nine studies were included in the quantitative synthesis, and separate meta‐analyses were conducted for the outcomes self‐reported craving and objective food intake. Results show small to medium positive effects across specific craving interventions on both outcomes. Effect sizes were partly moderated by intervention type. The most effective intervention regarding food intake was in sensu cue exposure. For subjective craving, the most robust evidence was found for beneficial effects of cognitive regulation strategies (ie, reappraisal, suppression, and distraction). Results further indicate that training inhibitory control through behavioral inhibition might be more effective than approach‐avoidance training when considering its effect on subjective craving and food intake. People with external eating habits, overeating, or loss‐of‐control eating might benefit from these types of specific craving interventions. Future research should focus on long‐term effects, transferability, and effectiveness in clinical samples.
EARLY HELLADIC I AND TALIOTI POTTERY: IS IT JUST A PHASE WE’RE GOING THROUGH?
Authors: Burke Clare , Day Peter M. , Kossyva Angeliki 
Published: January 2020

SummaryThis paper discusses the analysis of Early Bronze Age ‘Talioti’‐style ceramics found at sites in the Argolid and Corinthia, in the north‐east Peloponnese of mainland Greece. It presents the results of an integrated methodology that addresses questions relating to the potential sources of raw materials, the identification of potting practices, and the exchange of such vessels during the Early Bronze Age period. The work places site‐based results within a broader spatial and temporal context, specifically examining how this characteristic ceramic assemblage has been used as a chronological and cultural marker for the Early Bronze Age 1 (EB I) period in the north‐east Peloponnese. We argue that ‘Talioti’ pottery comprises the outcome of specific sets of knowledge, reputation and social practice in both its production and consumption, and that it is the determination of the location and method of production that allows an effective understanding of its chronological and spatial distribution. The case study is used to critically assess cultural and chronological interpretation of typological studies of ceramics, arguing that analytical understandings of provenance and technology are now fundamental to understanding both cultural identity and the passage of time.
Overexpression of GmAAP6a enhances tolerance to low nitrogen and improves seed nitrogen status by optimizing amino acid partitioning in soybean
Authors: Liu Sheng , Wang Dan , Mei Yuanyuan , Xia Tongmei , Xu Wei , Zhang Yuqing , You Xiang , Zhang Xiyu , Li Lei , Wang Ning Ning 
Published: January 2020

SummaryAmino acid transport via phloem is one of the major source‐to‐sink nitrogen translocation pathways in most plant species. Amino acid permeases (AAPs) play essential roles in amino acid transport between plant cells and subsequent phloem or seed loading. In this study, a soybean AAP gene, annotated as GmAAP6a, was cloned and demonstrated to be significantly induced by nitrogen starvation. Histochemical staining of GmAAP6a:GmAAP6a‐GUS transgenic soybean revealed that GmAAP6a is predominantly expressed in phloem and xylem parenchyma cells. Growth and transport studies using toxic amino acid analogs or single amino acids as a sole nitrogen source suggest that GmAAP6a can selectively absorb and transport neutral and acidic amino acids. Overexpression of GmAAP6a in Arabidopsis and soybean resulted in elevated tolerance to nitrogen limitation. Furthermore, the source‐to‐sink transfer of amino acids in the transgenic soybean was markedly improved under low nitrogen conditions. At the vegetative stage, GmAAP6a‐overexpressing soybean showed significantly increased nitrogen export from source cotyledons and simultaneously enhanced nitrogen import into sink primary leaves. At the reproductive stage, nitrogen import into seeds was greatly enhanced under both sufficient and limited nitrogen conditions. Collectively, our results imply that overexpression of GmAAP6a enhances nitrogen stress tolerance and source‐to‐sink transport and improves seed quality in soybean. Co‐expression of GmAAP6a with genes specialized in source nitrogen recycling and seed loading may represent an interesting application potential in breeding.
Successful engraftment of haploidentical bone marrow with post‐transplantation cyclophosphamide in patients with aplastic anemia
Authors: Dang Brian Norman , De Oliveira Satiro , Gray Ashley , Bowles LaVette , Moore Theodore Bruce 
Published: January 2020

AbstractPatients with severe aplastic anemia (SAA) may benefit from hematopoietic stem cell transplantation, but many of them lack a matched donor. Haploidentical transplantation is increasingly utilized for the treatment of nonmalignant disease where patients lack a matched donor. We report patients with aplastic anemia who experienced successful engraftments of haploidentical stem cells with post‐transplantation cyclophosphamide (PTCy). Case series and review of the literature. We present two cases of pediatric patients with severe aplastic anemia who experienced successful engraftment of haploidentical related bone marrow. Both patients received conditioning consisting of rabbit ATG, cyclophosphamide, fludarabine, and total body irradiation pretransplant, with PTCy. The conditioning regimen was well tolerated by both patients, and they achieved full donor engraftment and were weaned off all immunosuppressants. Haploidentical stem cell transplantation in patients with severe aplastic anemia may be an effective alternative when fully matched donors are not available. PTCy can facilitate successful engraftment and therefore expand the pool of eligible donors for patients with aplastic anemia.
Long‐term outcome of renal transplantation in childhood‐onset anti‐neutrophil cytoplasmic antibody–associated vasculitis
Authors: Nagasawa Takeshi , Miura Kenichiro , Kaneko Naoto , Yabuuchi Tomoo , Ishizuka Kiyonobu , Chikamoto Hiroko , Akioka Yuko , Hisano Masataka , Hattori Motoshi 
Published: January 2020

AbstractBackgroundThere have been a few reports of RTx for AAV in children; however, post‐transplant recurrence rate and long‐term prognosis remain unclear. Here, we describe the long‐term outcomes of RTx in childhood‐onset AAV.MethodsWe conducted a retrospective study of children who underwent RTx for AAV between 1999 and 2017 and had a follow‐up period of >2 years.ResultsSeven patients consisting of three children with MPA and four with RLV were analyzed. Age at Dx was 5.9 (median; range, 4.1‐14.5) years. PD was instituted in all patients, and median time on dialysis was 26 (range, 14‐63) months. Age at RTx was 12.8 (median; range, 8.7‐16.3) years. There were no recurrences of AAV noted during the median follow‐up period of 7.0 (range, 2.7‐18.8) years after RTx. Graft loss occurred in one patient due to non‐adherence. Estimated glomerular filtration rate of the remaining patients at the last follow‐up was 73.0 (median; range, 50.7‐93.9) mL/min/1.73 m2. No malignancies and deaths occurred during the observational period.ConclusionsOur study suggests that RTx for AAV with ESRD is a potentially safe and effective treatment choice for children with AAV.
Quantitative ultrasound elastography and biometry of the bitch uterus in the early puerperium after vaginal delivery and caesarean section
Authors: Simões Ana Paula Rodrigues , Maronezi Marjury Cristina , Uscategui Ricardo Andres Ramirez , Avante Michelle Lopes , Gasser Beatriz , Silva Priscila , Pavan Letícia , Maciel Giovanna Serpa , Pelógia Marina Emanoella Seruti , Vicente Wilter Ricardo Russiano , Feliciano Marcus Antonio Rossi 
Published: January 2020

AbstractElastography is an actual imaging method used to qualitatively and quantitatively evaluate the elastic properties of tissues. The aim of this study was to evaluate and compare uterine tissue biometry and stiffness during post‐partum period in brachycephalic bitches (n = 12) after c‐section (GCS; n = 8) or normal delivery (GNB; n = 4). These animals were evaluated daily by abdominal ultrasound from the day of delivery until the 10th day post‐partum; measuring uterine diameter, myometrial and endometrial thickness (mm) and shear wave velocity (SWV; m/s), by B‐mode and ARFI (acoustic radiation force impulse) elastography, respectively. Uterine diameter was higher (p = .012) in animals submitted to c‐section (15.26 ± 4.73 mm) than in normal birth (12.53 ± 2.64 mm) during the first 7 days post‐partum. Uterine thickness gradually involuted in both groups (p < .0001), the myometrium during the first 9 days (p = .005) and the endometrium during the first 6 days (p = .003). The myometrial and endometrial SWVs were similar between types of delivery (p = .7846 and .8273) and presented a gradual increase (p = .411; .0043, respectively), during the first 10 days post‐partum. It was concluded that bitches with normal delivery had smaller uterine thickness and faster puerperal involution than submitted to c‐section, while uterine tissue stiffness was similar between delivery types and increased gradually during post‐partum.
Carbonate slope re‐sedimentation in a tectonically‐active setting (Western Sicily Cretaceous Escarpment, Italy)
Authors: Randazzo Vincenzo , Le Goff Johan , Di Stefano Pietro , Reijmer John , Todaro Simona , Cacciatore Maria Simona 
Published: January 2020

AbstractTectonic processes are widely considered as a mechanism causing carbonate platform margin instabilities leading to the emplacement of mass transport deposits and calciturbidites. However, only few examples establishing a clear link between tectonics and re‐sedimentation processes are known from the literature. The two‐dimensional and three‐dimensional wire‐cut walls of hundreds of quarries extracting ornamental limestones (for example, Perlato di Sicilia) from the Western Sicily Cretaceous Escarpment in Italy expose a series of mass transport deposits. The depositional architecture, spatial facies distribution and sedimentary features of these deposits were studied in detail. Thin section analysis was used to define the microfacies characteristics and to determine the age of the re‐sedimented limestones. Eleven facies types grouped into four facies associations were recognized that defined specific depositional processes and environments. The stratigraphic architecture of the slope was reconstructed using four composite facies successions based on the detailed analysis and correlation of the field sections. The palaeoslope orientation was reconstructed based on the analysis of synsedimentary faults, slump scars and pinch‐out geometries. The Western Sicily Cretaceous Escarpment was strongly influenced by synsedimentary transtensional tectonics in combination with magmatic processes, as suggested by the presence of tuffites and pillow lava intercalations within the re‐sedimented carbonate series. These volcanics point to a major role of crustal shear as a trigger for mass transport deposit emplacement. The facies distribution along the Western Sicily Cretaceous Escarpment delivers new insights into the deformation processes accompanying the crustal extension of the Cretaceous western Tethys realm.
ATP binding cassette transporters ABCG1 and ABCG16 affect reproductive development via auxin signalling in Arabidopsis
Authors: Liu Liping , Zhao Lihua , Chen Piaojuan , Cai Hanyang , Hou Zhimin , Jin Xingyue , Aslam Mohammad , Chai Mengnan , Lai Linyi , He Qing , Liu Yanhui , Huang Xiaoyi , Chen Huihuang , Chen Yingzhi , Qin Yuan 
Published: January 2020

SummaryAngiosperm reproductive development is a complex event that includes floral organ development, male and female gametophyte formation and interaction between the male and female reproductive organs for successful fertilization. Previous studies have revealed the redundant function of ATP binding cassette subfamily G (ABCG) transporters ABCG1 and ABCG16 in pollen development, but whether they are involved in other reproductive processes is unknown. Here we show that ABCG1 and ABCG16 were not only expressed in anthers and stamen filaments but also enriched in pistil tissues, including the stigma, style, transmitting tract and ovule. We further demonstrated that pistil‐expressed ABCG1 and ABCG16 promoted rapid pollen tube growth through their effects on auxin distribution and auxin flow in the pistil. Moreover, disrupted auxin homeostasis in stamen filaments was associated with defective filament elongation. Our work reveals the key functions of ABCG1 and ABCG16 in reproductive development and provides clues for identifying ABCG1 and ABCG16 substrates in Arabidopsis.
Trauma‐induced primary hypoparathyroidism following severe bite wound injury to the neck in a dog
Authors: Wolf Johanna , Vigani Alessio , Schaer Michael 
Published: January 2020

AbstractObjectiveTo describe the clinical presentation and outcome of a dog with primary hypoparathyroidism secondary to cervical bite wounds.Case SummaryA 3‐year‐old male intact Chihuahua presented after being attacked by a large breed dog. The dog sustained severe cervical lacerations, exposing the trachea and jugular veins. A portion of the right thyroid gland was missing. The dog was stabilized before wound debridement and closure. Ionized calcium concentrations were within reference range at the time of presentation. Forty‐eight hours after the initial trauma, the dog was presented in lateral recumbency with signs of hypovolemic shock, muscle tremors, and hyperthermia. Bloodwork showed severe ionized hypocalcemia with low normal parathyroid hormone concentration consistent with acute primary hypoparathyroidism. The dog was managed initially with IV calcium gluconate and calcitriol, then long‐term oral calcium carbonate and vitamin D3. After 6 months, the dog was successfully weaned off calcium supplementation.New or Unique Information ProvidedThis is the first described case of traumatic primary hypoparathyroidism after a bite injury to the neck in a dog.
Effect of differential muscle activation patterns on muscle deoxygenation and microvascular haemoglobin regulation
Authors: Okushima Dai , Poole David C. , Barstow Thomas J. , Kondo Narihiko , Chin Lisa M. K. , Koga Shunsaku 
Published: January 2020

New Findings
What is the central question of this study?
Does the presence and extent of heterogeneity in the ratio of O2 delivery to uptake across human muscles relate specifically to different muscle activation patterns?

What is the main finding and its importance?
During ramp incremental knee‐extension and cycling exercise, the profiles of muscle deoxygenation (deoxy[haemoglobin + myoglobin]) and diffusive O2 potential (total[haemoglobin + myoglobin]) in the vastus lateralis corresponded to different muscle activation strategies. However, this was not the case for the rectus femoris, where muscle activation and deoxygenation profiles were dissociated and might therefore be determined by other structural and/or functional attributes (e.g. arteriolar vascular regulation and control of red blood cell flux).

AbstractNear‐infrared spectroscopy has revealed considerable heterogeneity in the ratio of O2 delivery to uptake as identified by disparate deoxygenation {deoxy[haemoglobin + myoglobin] (deoxy[Hb + Mb])} values in the exercising quadriceps. However, whether this represents a recruitment phenomenon or contrasting vascular and metabolic control, as seen among fibre types, has not been established. We used knee‐extension (KE) and cycling (CE) incremental exercise protocols to examine whether differential muscle activation profiles could account for the heterogeneity of deoxy[Hb + Mb] and microvascular haemoconcentration (i.e. total[Hb + Mb]). Using time‐resolved near‐infrared spectroscopy for the quadriceps femoris (vastus lateralis and rectus femoris) during exhaustive ramp exercise in eight participants, we tested the following hypotheses: (i) the deoxy[Hb + Mb] (i.e. fractional O2 extraction) would relate to muscle activation levels across exercise protocols; and (ii) KE would induce greater total[Hb + Mb] (i.e. diffusive O2 potential) at task failure (i.e. peak O2 uptake) than CE irrespective of muscle site. At a given level of muscle activation, as assessed by the relative integrated EMG normalized to maximal voluntary contraction (%iEMGmax), the vastus lateralis deoxy[Hb + Mb] profile was not different between exercise protocols. However, at peak O2 uptake and until 20% iEMGmax for CE, rectus femoris exhibited a lower deoxy[Hb + Mb] (83.2 ± 15.5 versus 98.2 ± 19.4 μm) for KE than for CE (P < 0.05). The total[Hb + Mb] at peak O2 uptake was not different between exercise protocols for either muscle site. These data support the hypothesis that the contrasting patterns of convective and diffusive O2 transport correspond to different muscle activation patterns in vastus lateralis but not rectus femoris. Thus, the differential deoxygenation profiles for rectus femoris across exercise protocols might be dependent upon specific facets of muscle architecture and functional haemodynamic events.