bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a prognostic signature based on six immune related lncRNAs for hepatocellular carcinoma.
Authors: Song Ze-bing , Zhang Guo-pei , li shaoqiang 
Published: March 2020

Abstract
Background: Hepatocellular carcinoma (HCC) is one of the most common malignant tumor in the world which prognosis is poor. Therefore, a precise biomarker is needed to guide treatment and improve prognosis. More and more studies have shown that lncRNAs and immune response are closely related to the prognosis of hepatocellular carcinoma. The aim of this study was to establish a prognostic signature based on immune related lncRNAs for HCC.Methods: Univariate cox regression analysis was performed to identify immune related lncRNAs, which had negative correlation with overall survival (OS) of 370 HCC patients from The Cancer Genome Atlas (TCGA). A prognostic signature based on OS related lncRNAs was identified by using multivariate cox regression analysis. Gene set enrichment analysis (GSEA) and a competing endogenous RNA (ceRNA) network were performed to clarify the potential mechanism of lncRNAs included in prognostic signature. Results: A prognostic signature based on OS related lncRNAs (AC145207.5, AL365203.2, AC009779.2, ZFPM2-AS1, PCAT6, LINC00942) showed moderately in prognosis prediction, and related with pathologic stage (Stage I&II VS Stage III&IV), distant metastasis status (M0 VS M1) and tumor stage (T1-2 VS T3-4). CeRNA network constructed 15 aixs among differentially expressed immune related genes, lncRNAs included in prognostic signature and differentially expressed miRNA. GSEA indicated that these lncRNAs were involved in cancer-related pathways. Conclusion: We constructed a prognostic signature based on immune related lncRNAs which can predict prognosis and guide therapies for HCC.
Bicondylar Tibial Plateau Fractures Involving the Posterior Fragment: Injury Mechanism and Fracture Characteristics
Authors: Pan Shuo , Peng Aqin , Hu Yaning , Wang Shuai , Zhang Xiao 
Published: March 2020

Abstract
Background: Bicondylar tibial plateau fracture (BTPF) involving the posterior fragment is the most complex intra-articular fracture to treat. Although the fracture characteristics of BTPFs have been reported by many researchers, due to the sophisticated kinematics of the knee, reports focused on the injury mechanism are still scarce. The current injury mechanism is insufficient to explain the various fracture characteristics and to guide surgery. This study used a three-dimensional (3D) simulation method to investigate the injury mechanism and fracture characteristics of BTPFs involving the posterior fragment. Methods: Ninety-six BTPFs involving the posterior fragment were included. A computer-assisted 3D technique, which enabled us to move the femoral condyles to match the displaced fracture fragments of the tibial plateau, was applied to analysis the injury mechanism for all cases. The 3D and two-dimensional (2D) morphology of each fracture were reviewed thoroughly, and the main fracture lines were mapped and superimposed on a template. Results: After simulation and quantitatively analysis, three main types (extension, flexion-internal rotation, flexion-external rotation) and six sub-types (valgus and varus in each main type) of injury mechanism had been classified according to the degree of knee motion in three planes. In the extension type, femoral condyles compress the anterolateral tibial plateau and central medial plateau, resulting in an anterior-to-posterior fracture line. In the flexion-internal rotation type, the compressed areas are located on the posterolateral and anteromedial plateau, forming an oblique anteromedial-to-posterolateral fracture line. In the flexion-external rotation type, the compressed areas are located on the anterolateral and posteromedial plateau, exhibiting an anterolateral-to-posteromedial fracture line. Conclusions: Different injury mechanisms result in different fracture characteristics. A thorough understanding of the injury mechanisms underlying complex BTPFs involving the posterior fragment is meaningful not only for fracture characteristics reorganization but also for surgical decision making. The 3D simulation method employed in this study may be a useful supplemental method for investigating the mechanisms underlying fracture injuries.
Prognostic value of Serum miR-217 Level in Osteosarcoma Patients
Authors: Ye Jia , Jin Zhi-Hui , Chen Ren , Chen Sen , Ren Yi-Jun , Guo Wei-Chun , He Hui 
Published: March 2020

Abstract
Background MicroRNA (miR)-217 is a tumor suppressor significantly associated with osteosarcoma. We try to evaluate serum levels miR-217 in osteosarcoma patients and evaluate its prognostic significance. Methods A total of 163 consecutive osteosarcoma patients and 96 healthy participates were enrolled. Serum miR-217 levels were evaluated by using real-time quantitative reverse transcription polymerase chain reactions(RT-PCR). The association between serum miR-217 level and survival outcomes was evaluated by univariate and multivariate analysis. Results Serum miR-217 levels in osteosarcoma patients was significantly lower than healthy volunteers (P<0.05). Low serum miR-217 was significantly related to advanced cancer and metastasis (both P<0.05). Moreover, patients with a low serum miR-217 had a poorer overall survival than those with a high serum miR-217 levels (P<0.05). Serum miR-217 level also been showed as independent risk factor for osteosarcoma in multivariate analysis (HR, 0.42; 95%CI: 0.12–0.98; p<0.01). Conclusions Serum miR-217 levels was significantly downregulated in osteosarcoma patients and remarkably associated with poor prognosis, indicating that serum miR-217 might serve as a useful diagnostic and prognostic indictor for osteosarcoma.
Practice and the Influencing Factors of Nurses Working in the Intensive Care Unit regarding Physical Restraint use: Multi-centered cross-sectional study
Authors: Gebeyehu Tilahun Kassew , Tilahun Ambaye Dejen , Wudu Bikis Liyew 
Published: March 2020

Abstract
Background: Nurses working in the intensive care unit commonly use physical restraint on critically ill patients. The main reason for the restraining of patients is to prevent the dislodgement of invasive procedures/ medical equipment, reduce agitation. Patients are exposed to different complications from physical restraint like worsening of agitation and delirium, fall injury, limb edema, restricted circulation, and skin laceration at restraint site, and death as a result of nurses’ improper practice regarding restraining. Despite this impact, there was no documented study in Ethiopia in this regard. Objective: To assess practice and its influencing factors of nurses working in the Intensive Care Unit regarding physical restraint Methods: An institution-based cross-sectional study was conducted from July to August 2019 at Amhara regional state referral hospitals, Northwest Ethiopia. All (260) nurses working in the intensive care unit were invited to participate through a convenience sampling technique. Structured questionnaires which consist of socio-demographic, professional characteristics and Level of Knowledge, Attitudes and Practices of Staff Regarding Physical Restraints Questionnaire were used. Simple and multiple linear regression and beta (β) coefficient at 95% confidence interval was employed with p-value<0.05 as statistical significance. Result: The mean score of nurses’ practice regarding physical restraint use among critically ill patients was 30.94±5.44. Nurses with short (≤2 years) experience of the intensive care unit, received educational training during graduate class regarding restraining, knowledge and attitude scores were the factors significantly associated with nurses’ practice score regarding physical restraining use. Conclusion: The practice regarding physical restraint use among critically ill patients among participant nurses’ was a satisfactory. The administrators’ should develop and provide educational and in-service training programs for nurses working in intensive care unit settings regarding physical restraint use and its alternatives, which is crucial for the nurses’ to improve their behavior of restraining. Keywords: Intensive care unit, Nurses’, Practice, Physical restraint
Compound heterozygous mutations of two eIF2B genes in early childhood onset form of vanishing white matter disease
Authors: Wang Chunmei , Yuan Fang , Wu Shengnan , Liu Zhao , Wang Simei , Lan Xiaoping , Xu Quanmei , Luo Xiaona , Wang Yilin , Xu Wuhen , Xi Jiaming , Lu Yanfen , Zhang Yuanfeng , Lu Qin , Huang Jianjun , Avula Sreenivas K. , Tolaymat Abdullah , Wang Anqi , Jia Jia , Chen Yucai 
Published: March 2020

Abstract
Background Diagnoses of vanishing white matter disease (VWMD) were difficult due to variable clinical features, severity, age of onset and wide range of mutations in eIF2G genes which cause VWMD. This study reported two novel mutations in eIF2B genes associated with VWMD to and expand our understanding of VWMD. Case presentation Relevant data from clinical diagnoses and genetic mutational analyses in two Chinese female patients with sporad­ic VWMD were collected and analyzed. Protein structure/function was predicted. The identity of biological parents was confirmed based on variants called from the next-generation sequencing (NGS) data. Compound heterozygous mutations, c.254 T>A (p.Val85Glu), and c.597+2delT in the EIF2B2 gene, c.545C>T(p.Thr182Met) and c.1340C>T(p.Ser447Leu) in the EIF2B5 gene were detected in the two patients. Further phenotype investigation of both patients enables the diagnosis of the vanishing white matter disease .Three missense mutation c.254 T>A (p.Val85Glu) in the EIF2B2 gene, c.545C>T(p.Thr182Met) and c.1340C>T(p.Ser447Leu) in the EIF2B5 gene have been found and predicted to be deleterious. All the three mutation causes hydrophobicity and stability changes of proteins, and all the mutations were localized in conserved sequences. One novel mutation of c.1340C>T(p.Ser447Leu) in the EIF2B5 gene ,and two other known mutations. The iterative threading assembly refinement (I-TASSER) server generated three-dimensional (3D) atomic models based on protein sequences from the novel missense mutation of c.1340C>T(p.Ser447Leu) in the EIF2B5 gene, which showed that the protein structure changed . The novel mutation c.597+2delT in the EIF2B2 gene may cause the splice site to disappear. We also analyzed mutations in missense mutations that cause VWMD and found that most of the t Pathogenic sites are localized in conserved NT and I-patch homology regions and the catalytic domain of EIF2Bε. Conclusions This study expands the spectrum of genotypes and phenotypes of VWMD and provides new insights into the molecular mechanism of VWMD and aide the acute diagnosis and treatment of VWMD.
Non-invasive ventilation as the initial ventilatory strategy in very old critically ill patients with community-acquired pneumonia: a multicenter cohort study
Authors: Besen Bruno Adler Maccagnan Pinheiro , Park Marcelo , Ranzani Otavio Tavares 
Published: March 2020

Abstract
Background The very old patients (≥ 80 years-old, VOP) comprise a subpopulation increasingly admitted to intensive care units (ICUs). Community-acquired pneumonia (CAP) is a common reason for admission and the best strategy of mechanical ventilation for respiratory failure in this scenario is not fully known. Methods Multicenter cohort study of VOPs admitted with CAP in need of invasive (IMV) or noninvasive (NIV) mechanical ventilation to 11 Brazilian ICUs from 2009 through 2012. We used logistic regression models to evaluate the association between ventilator strategy (NIV vs. IMV) and hospital mortality adjusting for confounding factors. We evaluated effect modification with interaction terms in pre-specified sub-groups. Results Of 369 VOPs admitted for CAP with respiratory failure, 232 (63%) received NIV and 137 (37%) received IMV as initial ventilatory strategy. IMV patients were sicker at ICU admission (median SOFA 8 vs. 4, p < 0.001). Hospital mortality was 114/232 (49%) for NIV and 90/137 (66%) for IMV. For the comparison NIV vs. IMV (reference), the crude odds ratio (OR) was 0.50 (95% CI, 0.33–0.78, p=0.002). This association was largely confounded by antecedent characteristics and non-respiratory SOFA (adjOR = 0.70, 95% CI, 0.41–1.20, p=0.196). The fully adjusted model, including Pao2/Fio2 ratio, pH and Paco2, yielded an adjOR of 0.81 (95% CI, 0.46–1.41, p=0.452). There was no strong evidence of effect modification among relevant subgroups, such as Pao2/Fio2 ratio ≤ 150 (p = 0.30), acute respiratory acidosis (p = 0.42) and non-respiratory SOFA ≥ 4 (p = 0.53). Conclusions NIV was not associated with lower hospital mortality when compared to IMV in critically ill VOP admitted with CAP, but there was no strong signal of harm from its use. The main confounders of this association were both the severity of respiratory dysfunction and of extra-respiratory organ failures.
Identification of stable reference gene for qPCR studies in common wheat (Triticum aestivum L.) seedlings under short-term osmotic stress
Authors: Dudziak Karolina , Sozoniuk Magdalena , Börner Andreas , Szczerba Hubert , Kuzdraliński Adam , Kowalczyk Krzysztof , Nowak Michał 
Published: March 2020

Abstract
BackgroundQuantitative PCR (qPCR) is one of the most common and accurate methods of gene expression analysis. However, the biggest challenge for this kind of examinations is normalization of the results, which requires the application of dependable internal controls. The selection of appropriate reference genes (RGs) is one of the most crucial points in qPCR data analysis and for correct assessment of gene expression. Because of the fact that many reports indicate that the expression profiles of typically used RGs can be unstable in certain experimental conditions, species or tissues, reference genes with stable expression levels should be selected individually for each experiment. In this study, we analysed a set of ten candidate RGs for wheat seedlings under short-term drought stress. Our tests included five ‘traditional’ RGs (GAPDH, ACT, UBI, TUB, and TEF1) and five novel genes developed by the RefGenes tool from the Genevestigator database.ResultsExpression stability was assessed using five different algorithms: geNorm, NormFinder, BestKeeper, RefFinder and the delta Ct method. In the final ranking, we identified three genes: CJ705892, ACT, and UBI, as the best candidates for housekeeping genes. However, our data indicated a slight variation between the different algorithms that were used. We revealed that the novel gene CJ705892, obtained by means of in silico analysis, showed the most stable expression in the experimental tissue and condition.ConclusionsOur results support the statement, that novel genes selected for certain experimental conditions have a more stable level of expression in comparison to routinely applied RGs, like genes encoding actin, tubulin or GAPDH. Selected CJ705892 gene can be used as a housekeeping gene in the expression analysis in wheat seedlings under short-term drought. The results of our study will be useful for subsequent analyses of gene expression in wheat tissues subjected to drought.
Prevalence of depressive symptoms and its predicted factors among medical students in University of Bisha, Saudi Arabia
Authors: Al-Shahrani Mohammad S. , Alharthi Muffarah H. , Alamri Mohannad S. , Ibrahim Mutasim E 
Published: March 2020

Abstract
Background: Identification of the potential factors of depression among medical students is the first step towards academic excellence and future safe medical practice. Methods: A cross-sectional study was conducted during a period from November 2019 to February 2020 at the University of Bisha, College of Medicine (UBCOM), Bisha province, Saudi Arabia. Medical students from year one to year six were involved. A self-administered questionnaire was used to collect data about socio-demographic and academic characteristics of students. The Arabic version of the PHQ-9 scale with a score of ≥10 was used for diagnosis depressive. Logistic regression analysis was used to assess the prevalence and risk of depression.Results: Of the 190 students enrolled, 26.8% had depressive symptoms, of which 45.1% were having moderate to severe symptoms. The highest proportion of depression was found among second-year students (43.8%), followed by sixth-year students (41.2%), whereas lowest proportion of depression reported among first-year students (8.9%). Univariate regression revealed significant associations between depression and un-satisfaction of family income, loss of family members, having psychological illness, difficulties in personal relationships, regretted study medicine, failure in academic year, a lower grade than expected, conflict with tutors, lack of college facilities and heavy academic load. In multivariate analysis, loss of a family members (AOR=0.077; 95% CI 0.013-0.451), difficulties in personal relationships (AOR=2.371; 95% CI 1.009-5.575), regret study medicine (AOR=3.764; 95% CI 1.657-8.550), failed in academic year (AOR=2.559; 95% CI 1.112-5.887) were independently associated with depression.Conclusion: The study concluded that medical students at UBCOM also experience the existence of depressive symptoms associated with various risk indicators. Optimizing the educational and social environment coupled with infrastructure facilities at UBCOM might promote students' mental health and well-being.
Microbial community similarity and dissimilarity inside and across full-scale activated sludge processes for simultaneous nitrification and denitrification
Authors: Wen Jianfeng , LeChevallier Mark W. , Tao Wendong 
Published: March 2020

Abstract
Simultaneous nitrification and denitrification under low dissolved oxygen conditions is an energy-saving modification of the activated sludge process to achieve efficient nitrogen removal. Geographically distinct full-scale treatment plants are excellent platforms to address the links of microbial community with operating parameters. Mixed liquor samples were collected from a sequencing batch reactor plant, oxidation ditch plant, and step-feed activated sludge plant. Next-Generation Sequencing of the samples showed that the microbial communities were similar at the phylum level among the plants, being dominated by Proteobacteria. Microbial composition of functional groups was similar between the react fill and react phases of the sequencing batch reactors, among four sequencing batch reactors, and among four oxidation ditches. Nitrospira was the only identified genus of autotropic nitrifying bacteria with a relative abundance of 2.2–2.5% in the oxidation ditches and 0.4–0.7% at the other plants. Heterotrophic nitrifying–aerobic denitrifying bacteria were dominated by Dechloromonas with a relative abundance of 0.4–1.0%. Microbial community composition and nitrogen removal mechanisms were related to overall level and local zonation of dissolved oxygen, mixed liquor suspended solids concentration, nitrogen and organic loadings, and solids retention time. Low dissolved oxygen and low organic and nitrogen loadings favored growth of Nitrospira.
A Mobile Just-in-Time Adaptive Intervention for Smoking Cessation: Pilot Randomized Controlled Trial
Authors: Hébert Emily T , Ra Chaelin K , Alexander Adam C , Helt Angela , Moisiuc Rachel , Kendzor Darla E , Vidrine Damon J , Funk-Lawler Rachel K , Businelle Michael S 
Published: March 2020

Background
Smartphone apps for smoking cessation could offer easily accessible, highly tailored, intensive interventions at a fraction of the cost of traditional counseling. Although there are hundreds of publicly available smoking cessation apps, few have been empirically evaluated using a randomized controlled trial (RCT) design. The Smart-Treatment (Smart-T2) app is a just-in-time adaptive intervention that uses ecological momentary assessments (EMAs) to assess the risk for imminent smoking lapse and tailors treatment messages based on the risk of lapse and reported symptoms.

Objective
This 3-armed pilot RCT aimed to determine the feasibility and preliminary efficacy of an automated smartphone-based smoking cessation intervention (Smart-T2) relative to standard in-person smoking cessation clinic care and the National Cancer Institute’s free smoking cessation app, QuitGuide.

Methods
Adult smokers who attended a clinic-based tobacco cessation program were randomized into groups and followed for 13 weeks (1 week prequitting through 12 weeks postquitting). All study participants received nicotine patches and gum and were asked to complete EMAs five times a day on study-provided smartphones for 5 weeks. Participants in the Smart-T2 group received tailored treatment messages after the completion of each EMA. Both Smart-T2 and QuitGuide apps offer on-demand smoking cessation treatment.

Results
Of 81 participants, 41 (50%) were women and 55 (68%) were white. On average, participants were aged 49.6 years and smoked 22.4 cigarettes per day at baseline. A total of 17% (14/81) of participants were biochemically confirmed 7-day point prevalence abstinent at 12 weeks postquitting (Smart-T2: 6/27, 22%, QuitGuide: 4/27, 15%, and usual care: 4/27, 15%), with no significant differences across groups (P>.05). Participants in the Smart-T2 group rated the app positively, with most participants agreeing that they can rely on the app to help them quit smoking, and endorsed the belief that the app would help them stay quit, and these responses were not significantly different from the ratings given by participants in the usual care group.

Conclusions
Dynamic smartphone apps that tailor intervention content in real time may increase user engagement and exposure to treatment-related materials. The results of this pilot RCT suggest that smartphone-based smoking cessation treatments may be capable of providing similar outcomes to traditional, in-person counseling.

Trial Registration
ClinicalTrials.gov NCT02930200; https://clinicaltrials.gov/show/NCT02930200
Duplicaciones del tracto gastrointestinal en niños, presentación de dos casos. Hospital Infantil Doctor Robert Reid Cabral (HIRRC), República Dominicana
Authors: Perez Manuel , Carrasco Aura , Cabrera Valerio Carlos , Pérez Enmanuel , Féliz Erick , Toral Eliana 
Published: March 2020

Introducción: la duplicación gastrointestinal es una ano-malía relativamente rara que puede ocurrir en cualquier nivel desde la cavidad bucal hasta el recto, los casos de duplicación gástrica son aún más raros, y la mayoría de ellos se han reportado en niños.
Material y método: en el siguiente trabajo se expone dos casos; número 1: paciente de sexo femenino de 2 años de edad, con presentación clínica de vómitos de contenido alimentario que luego se tornaron biliosos en número de 3-4 ocasiones por día, de 2 días de evolución, dolor abdo-minal difuso de 2 días de evolución, masa abdominal localizada en hemiabdomen derecho. Caso número 2: paciente masculino de 4 días de vida, a quien se realiza ultrasonido obstétrico a las 34 semanas de gestación, donde se evidencia imagen quística nivel abdominal. A ambos casos se le realiza laparotomía exploratoria eviden-ciando en caso número 1: duplicación gástrica al que se le realiza exéresis total y rafia gástrica, y el caso número 2: malrotación intestinal con vólvulo de intestino medio más duplicación intestinal (ileal), al que se le aplica proce-dimiento de Ladd más resección y anastomosis termino terminal ileoileal, ambos pacientes evolucionan de manera satisfactoria.
Conclusión: el propósito consiste en describir la presen-tación de ambos casos en la infancia, lo importante de los métodos diagnósticos y de la corrección quirúrgica por diferentes técnicas según la localización del quiste en el tracto gastrointestinal.