bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reconocimiento de la expresión facial de la emoción en mexicanos universitarios
Authors: Anguas-Wong Ana María , Matsumoto David 
Published: September 2020

Este estudio explora el reconocimiento de la expresión facial de las emociones en bilingües mexicanos mediante el JACFEE (Matsumoto & Ekman, 1988). Investigaciones previas evidencian el alto nivel de acuerdo transcultural en el reconocimiento emocional, sin embargo no se reportan estudios en la cultura mexicana. Participaron 229 estudiantes universitarios, edad promedio 21.79 años. Los resultados indican que las emociones universales: enojo, desprecio, disgusto, temor, felicidad, tristeza y sorpresa fueron reconocidas más allá del azar (p < .01), independientemente del sexo o nacionalidad del modelo. Estos hallazgos coincidencompletamente con los datos transculturales que se tienen sobre el alto nivel de acuerdo en el reconocimiento emocional (Ekman, 1994), contribuyendo así al creciente cuerpo de evidencia sobre la universalidad de las emociones.
Detectando el perfil simulador en el MMPI-2: una propuesta basada en la investigación
Authors: Sánchez Crespo Guadalupe , Jiménez Gómez Fernando , Ampudia Rueda Amada 
Published: September 2020

Se propone un perfil detector de las falsificaciones que se dan con el MMPI-2, proponiendo la inversión de las puntuaciones directas de L y K y añadiendo cuatro nuevas escalas específicas: Odecp, Ds-r, S, F-K. Para esto se formaron dos grupos: control, compuesto por los subgrupos normal y clínico que contestan de forma estándar al MMPI-2, y experimental, formado por tres subgrupos instruidos para contestar al MMPI-2 de una manera determinada: mostrando buena imagen, mala imagen y de forma inconsistente. El resultado de este estudio es un perfil con las escalas de validez propuestas para la detección de las distintas falsificaciones que pueden realizar los sujetos que han contestado al cuestionario del MMPI-2.
Enfoque de medios de vida: estrategias de los caficultores para enfrentar las presiones en el medio rural costarricense.
Authors: Alonso Ubieta Suyen , Delgado Ballesteros Antonio , Díaz Porras Rafael 
Published: September 2020

El objetivo de este artículo es identificar los capitales que inciden en la cantidad de estrategias adoptadas por familias costarricenses que optaron por la actividad de producción de café como un medio de vida. Sobre el diseño metodológico, los datos fueron recopilados durante el año 2010-2011, por medio de una muestra aleatoria de 399 productores de café ubicados en los cantones de León Cortes, Nicoya, Hojancha y Nandayure, Costa Rica. Se emplea la técnica de análisis factorial y un modelo de regresión binomial negativa para explicar la relación entre el número de estrategias (variable dependiente) y los capitales de las familias. Los resultados de la regresión explican que el capital humano (conocimientos y capacidades) y el capital natural y social explican el número de estrategias adoptadas por las familias. La limitación del modelo propuesto es que omite otras variables, como la vulnerabilidad del entorno (presiones exógenas), que podrían estar más relacionadas al número de estrategias para subsistir en el medio rural que a la misma acumulación de capitales.
Subasta ganadera evaluada como proyecto de inversión propuesta por la Cámara de Ganaderos de Upala.
Authors: Morales Abarca Ana Lucía , Álvarez Varela Diana Carolina , Sánchez Gómez José Ignacio , Almendarez Midence Jaime Rubén , Guillén Castro Siany Fabiola 
Published: September 2020

La Cámara de Ganaderos de Upala es una organización conformada por 140 productores debidamente registrados, con dos años de fundación. Creada con el fin de solidarizarse con los productores después del huracán Otto, ocurrido en 2016 afectando la zona Note de Costa Rica. El proyecto que se plantea es un análisis económico y técnico para el posible establecimiento de una subasta ganadera en la zona de Upala, Costa Rica para el año 2019. El elemento diferenciador sería un margen de comisión competitivo ante las subastas cercanas y una administración directa que beneficia a la cámara y a los ganaderos oferentes de ganado.
Se realizó mediante revisión de información secundaria, consulta a expertos, visitas programadas y generación de indicadores financieros, esto con el fin de generar un marco contextual de los alcances de la actividad desde un ángulo financiero y respecto a los principales requerimientos técnicos para una operación máxima de la actividad en estudio.
Los principales resultados generados de la investigación fueron: una inversión inicial de ₡13 717 644,65; donde no se incurre en desembolsos de infraestructura y otros rubros que aumentan esta partida, una tasa interna de retorno positiva de 32,92%, un valor actual neto de ₡2 615 472,31 derivado de la actividad mediante una tasa de costo de capital de 15,01% y una un período de retorno de 3,45 años.
Como recomendación, la implementación de este proyecto analizado desde los enfoques técnicos y económicos muestra que existe viabilidad para realizar el proyecto, el cual debido a su magnitud es una opción que potencia el fortalecimiento de la cámara y les brinda preparación para proyectos futuros de mayor tamaño.
The pore size of chitosan-Aloe vera scaffold and its effect on VEGF expressions and woven alveolar bone healing of tooth extraction of Cavia cobaya
Authors: Sularsih S. 
Published: September 2020

Background: Pore size of scaffolds affects cellular activity, stimulates angiogenetic factors of vascular endothelial growth factor (VEGF), synthesises new blood vessels to regulate migration and proliferation, and accelerates alveolar bone healing of tooth extraction. Purpose: This study aims to analyse the pore size of chitosan-Aloe vera scaffold and its effects on VEGF expression and woven alveolar bone healing of tooth extraction of Cavia cobaya. Methods: 36 male Cavia cobaya, aged 3-3.5 months were divided into six groups: negative control groups (without scaffold), positive control groups (chitosan scaffold), and treatment groups (chitosan-Aloe vera scaffold) on 7- and 14-day observations. Histopathological examination was performed to account the woven alveolar bone areas, and immunohistochemical examination was conducted to examine VEGF expressions on endothelial cells. Data was analysed using a one-way analysis of variance (ANOVA) and least significant difference (LSD) test (p<0.05). Scaffold pore size examination was performed with scanning electron microscope (SEM) with 250x and 500x magnification. Results: Chitosan-Aloe vera scaffold was found to have open pore interconnectivity, the largest pore size was 138.9 μm, while the smallest was 110.5 μm and average pore size was 134.85 μm. The highest expression of VEGF was observed in the treatment group on days 7 (11.5 ±1.39) and 14 (15.28±1.78), while the largest woven alveolar bone was observed in the treatment group on days 7(17.83±1.47) and 14 (37.67±3.65). Statistically, there was a significant difference between control groups and the treatment groups (p=0.000; p<0.05). Conclusion: Chitosan-Aloe vera scaffold has pore characteristics increasing VEGF expressions and woven alveolar bone areas.
Polymethyl Methacrylate Cement Fill as a Definitive Treatment for Massive Bone Defect After Infected Internal Fixation in Bicondylar Tibial Fracture
Authors: Reito Aleksi , Ylitalo Antti 
Published: September 2020

Case:
A 62-year-old woman sustained a bicondylar proximal tibial fracture. She had a deep infection after open reduction and internal fixation resulting in massive metaphyseal bone loss. Because only subchondral bone was left, the cavity was filled with cement after pins were used to support the proximal tibia. Then, a hinged knee-spanning external fixator (EF) was applied. After the removal of the EF, the patient was almost pain free and tolerated full weight-bearing, and no further surgeries were needed.

Conclusion:
The cemented wires technique proved to be a good short-term and possibly a reasonable long-term treatment approach for infected nonunion with massive bone loss still maintaining lower leg alignment.
Atypical Subtrochanteric Femur Fracture and Hip Osteoarthritis Treated with a Revision Monoblock Stem and Strut Allograft
Authors: Heckmann Nathanael D. , Yang JaeWon , DeBenedetti Anne , Della Valle Craig J. 
Published: September 2020

Case:
A 73-year-old woman was diagnosed with a nondisplaced incomplete atypical bisphosphonate-related subtrochanteric femur fracture and ipsilateral hip osteoarthritis. She was treated with a total hip arthroplasty using a monoblock revision stem and a strut allograft to provide adjunctive mechanical and biological support. At the final follow-up, the patient had no pain, the stem was well fixed, and the allograft strut had incorporated; however, a persistent fracture line was noted.

Conclusion:
When an atypical femur fracture coexists with hip osteoarthritis, total hip arthroplasty may be a reasonable treatment option to address the fracture and concomitant hip osteoarthritis with a single surgical intervention. However, consideration should be given to using a stem with distal fixation and augmenting the construct with a strut allograft.
An updated explanation of ancestral karyotype changes and reconstruction of evolutionary trajectories to form Camelina sativa chromosomes
Authors: Zhang Zhikang , Meng Fanbo , Sun Pengchuan , Yuan Jiaqing , Ge Ke , Liu Chao , Wang Weijie , Wang Xiyin 
Published: September 2020

Abstract
Background: Belonging to lineage Ⅰ of Brassicaceae, Camelina sativa is formed by two hybridizations of three species (three sub-genomes). The three sub-genomes were diverged from a common ancestor, likely derived from lineage Ⅰ (Ancestral Crucifer karyotype, ACK). The karyotype evolutionary trajectories of the C. sativa chromosomes are currently unknown. Here, we managed to adopt a telomere-centric theory proposed previously to explain the karyotype evolution in C. sativa. Results: By characterizing the homology between A. lyrata and C. sativa chromosomes, we inferred ancestral diploid karyotype of C. sativa (ADK), including 7 ancestral chromosomes, and reconstructed the evolutionary trajectories leading to the formation of extant C. sativa genome. The process involved 2 chromosome fusions. We found that sub-genomes Cs-G1 and Cs-G2 may share a closer common ancestor than Cs-G3. Together with other lines of evidence from Arabidopsis, we propose that the Brassicaceae plants, even the eudicots, follow a chromosome fusion mechanism favoring end-end joining of different chromosomes, rather than a mechanism favoring the formation circular chromosomes and nested chromosome fusion preferred by the monocots. Conclusions: The present work will contribute to understanding the formation of C. sativa chromosomes, providing insight into Brassicaceae karyotype evolution.
Genome wide analysis reveals genetic divergence between Goldsinny wrasse populations
Authors: Jansson Eeva , Besnier Francois , Malde Ketil , André Carl , Dahle Geir , Glover Kevin A. 
Published: September 2020

Abstract
Background: Marine fish populations are often characterized by high levels of gene flow and correspondingly low genetic divergence. This presents a challenge to define management units. Goldsinny wrasse (Ctenolabrus rupestris) is a heavily exploited species due to its importance as a cleaner-fish in commercial salmonid aquaculture. However, at the present, the population genetic structure of this species is still largely unresolved. Here, full-genome sequencing was used to produce the first genomic reference for this species, to study population-genomic divergence among four geographically distinct populations, and, to identify informative SNP markers for future studies. Results: After construction of a de novo assembly, the genome was estimated to be highly polymorphic and of ~600Mbp in size. 33 235 SNPs were thereafter selected to assess genomic diversity and differentiation among four populations collected from Scandinavia, Scotland, and Spain. Global FST among these populations was 0.015–0.092. Approximately 4% of the investigated loci were identified as putative global outliers, and ~1% within Scandinavia. SNPs showing large divergence (FST>0.15) were picked as candidate diagnostic markers for population assignment. 173 of the most diagnostic SNPs between the two Scandinavian populations were validated by genotyping 47 individuals from each end of the species’ Scandinavian distribution range. 69 of these SNPs were significantly (p<0.05) differentiated (mean FST_173_loci=0.065, FST_69_loci=0.140). Using these validated SNPs, individuals were assigned with high probability (≥ 94%) to their populations of origin.Conclusions: Goldsinny wrasse displays a highly polymorphic genome, and substantial population genomic structure. Diversifying selection likely affects population structuring globally and within Scandinavia. The diagnostic loci identified now provide a promising and cost-efficient tool to investigate goldsinny wrasse populations further.
Once the child is delivered, he is no more your baby,” Exclusive Breastfeeding experiences of first-time mothers in Kassena-Nankana Municipality, Ghana - a qualitative study
Authors: Adda Louisa , Opoku-Mensah Kwabena , Dako-Gyeke Phyllis 
Published: September 2020

Abstract
Background Exclusive Breastfeeding (EBF) for the first six months of life is globally accepted as the preferred method for infant feeding. In Ghana, an estimated 84% of children < 2 months old are exclusively breastfed. But by age 4 to 5 months, only 49% continue to receive EBF. This situation continues to deteriorate. Thus, the need to explore perceptions, practices as well as factors that influence EBF in Ghana. Methods Using a qualitative design, four focus group discussions were conducted among first-time mothers and eight in-depth interviews with health workers and traditional birth attendants. The study was conducted in four communities in the Kassena-Nankana municipality of Ghana. Discussions and interviews were recorded and later transcribed verbatim to English language. The transcribed data was then coded with the aid of analysis computer software (Nvivo version 10.0) and later analyzed for the generation of themes. Results Exclusive breastfeeding is practiced among first-time mothers due to its perceived benefits; which include nutritional advantage, ability to enhance growth whilst boosting immunity and its economic value. However misconceptions as well as, certain cultural practices (e.g. giving herbal concoctions, breastmilk purification rites), and relational influences, may threaten a mother’s intention to exclusively breastfeed. Relational influences are mainly from mother in-laws, traditional birth attendants, grandmothers, herbalists and other older adults in the community. Conclusions Although first time mothers attempt EBF, external influences make it practically challenging. The availability and utilization of information on EBF was found to positively influence perceptions towards EBF, leading to change in attitude towards the act. Thus, the practice of community-based health services may be strengthened to provide support for first-time mothers as well as continuous education to the mother in laws, female elders and community leaders who influence decision making on breastfeeding of infants
Liver fibrosis assessments using FibroScan, Virtual-Touch tissue quantification, the FIB-4 index, and Mac-2 binding protein glycosylation isomer levels compared with pathological findings of liver resection specimens in patients with hepatitis C infection
Authors: Ueda Naoyuki , Kawaoka Tomokazu , Imamura Michio , Aikata Hiroshi , Nakahara Takashi , Murakami Eisuke , Tsuge Masataka , Hiramatsu Akira , HAYES C.NELSON , Yokozaki Michiya , Chayama Kazuaki 
Published: September 2020

Abstract
BackgroundEvaluation of fibrosis stage is important to monitor progression of liver disease and risk of hepatocellular carcinoma (HCC). While liver biopsy is the gold standard, the method is invasive and faces several limitations. The aim of this study was to determine correlations among METAVIR scores and FibroScan, Virtual-Touch tissue quantification (VTQ), fibrosis index based on four factors (FIB-4 index), and Mac-2 binding protein glycosylation isomer (M2BPGi) level , and for examine differences in the reliability of non-invasive methods to evaluate fibrosis.MethodsWe used liver resection specimens from patients with hepatitis C virus (HCV), correlations were assessed between METAVIR scores and non-invasive method. Receiver operating characteristic (ROC) curves were generated to determine the sensitivity, specificity, and cut off values of the methods.ResultsAll Patients group: In F0-2 vs F3-4, the areas under the ROC curve (AUC) (0.85) of FibroScan was significantly higher than that (0.67) of FIB-4 index (p=0.002) and that (0.67) of M2BPGi (p=0.001). The AUC (0.83) of VTQ was significantly higher than that (0.67) of FIB-4 index (p=0.01) and that (0.67) of M2BPGi (p=0.002). In F0-3 vs F4, the AUC (0.86) of VTQ was significantly higher than that (0.65) of FIB-4 index (p=0.04). The AUC (0.89) of FibroScan was significantly higher than that (0.65) of FIB-4 index (p=0.002) and that (0.76) of M2BPGi (p=0.02).Non-SVR group: In F0-2 vs F3-4, the AUC (0.85) of FibroScan was significantly higher than that (0.84) of FIB-4 index (p=0.02) and that (0.73) of M2BPGi (p=0.003). The AUC (0.84) of VTQ was significantly higher than that (0.74) of FIB-4 index (p=0.04). In F0-3 vs F4, the AUC (0.91) of FibroScan was significantly higher than that (0.67) of FIB-4 index (p=0.003) and that (0.78) of M2BPGi (p=0.02). The AUC (0.88) of VTQ was significantly higher than that of FIB-4 index (0.67) and that of M2BPGi (0.78) (p=0.04).ConclusionsFibroScan and VTQ best reflected the results of hepatic fibrosis diagnosis using liver resection specimens among the four examination methods evaluated.
DeepGRN: Prediction of transcription factor binding site across cell-types using attention-based deep neural networks&nbsp;
Authors: Chen Chen , Hou Jie , Shi Xiaowen , Yang Hua , Birchler James A. , Cheng Jianlin 
Published: September 2020

Abstract
BackgroundDue to the complexity of the biological systems, the prediction of the potential DNA binding sites for transcription factors remains a difficult problem in computational biology. Genomic DNA sequences and experimental results from parallel sequencing provide available information about the affinity and accessibility of genome and are commonly used features in binding sites prediction. The attention mechanism in deep learning has shown its capability to learn long-range dependencies from sequential data, such as sentences and voices. Until now, no study has applied this approach in binding site inference from massively parallel sequencing data. The successful applications of attention mechanism in similar input contexts motivate us to build and test new methods that can accurately determine the binding sites of transcription factors.ResultsIn this study, we propose a novel tool (named DeepGRN) for transcription factors binding site prediction based on the combination of two components: single attention module and pairwise attention module. The performance of our methods is evaluated on the ENCODE-DREAM in vivo Transcription Factor Binding Site Prediction Challenge datasets. The results show that DeepGRN achieves higher unified scores in 6 of 13 targets than any of the top four methods in the DREAM challenge. We also demonstrate that the attention weights learned by the model are correlated with potential informative inputs, such as DNase-Seq coverage and motifs, which provide possible explanations for the predictive improvements in DeepGRN.ConclusionsDeepGRN can automatically and effectively predict transcription factor binding sites from DNA sequences and DNase-Seq coverage. Furthermore, the visualization techniques we developed for the attention modules help to interpret how critical patterns from different types of input features are recognized by our model.
Flagella by numbers: comparative genomic analysis of the supernumerary flagellar systems among the Enterobacterales
Authors: De Maayer Pieter , Pillay Talia , Coutinho Teresa A 
Published: September 2020

Abstract
Background: Flagellar motility is an efficient means of movement that allows bacteria to successfully colonize and compete with other microorganisms within their respective environments. The production and functioning of flagella is highly energy intensive and therefore flagellar motility is a tightly regulated process. Despite this, some bacteria have been observed to possess multiple flagellar systems which allow distinct forms of motility. Results: Comparative genomic analyses showed that, in addition to the previously identified primary peritrichous (flag-1) and secondary, lateral (flag-2) flagellar loci, three novel types of flagellar loci, varying in both gene content and gene order, are encoded on the genomes of members of the order Enterobacterales. The flag-3 and flag-4 loci encode predicted peritrichous flagellar systems while the flag-5 locus encodes a polar flagellum. In total, 798/4,028 (~20%) of the studied taxa incorporate dual flagellar systems, while nineteen taxa incorporate three distinct flagellar loci. Phylogenetic analyses indicate the complex evolutionary histories of the flagellar systems among the Enterobacterales. Conclusions: Supernumerary flagellar loci are relatively common features across a broad taxonomic spectrum in the order Enterobacterales. Here, we report the occurrence of five (flag-1 to flag-5) flagellar loci on the genomes of enterobacterial taxa, as well as the occurrence of three flagellar systems in select members of the Enterobacterales. Considering the energetic burden of maintaining and operating multiple flagellar systems, they are likely to play a role in the ecological success of members of this family and we postulate on their potential biological functions.
Pharmacokinetic herb-drug interactions between Aidi injection and Doxorubicin in rats with diethylnitrosamine-induced hepatocellular carcinoma
Authors: Lu Yuan , Pan Jie , Zhu Xiaoqing , Zhang Shuai , Chunhua Liu , Sun Jia , Li Yueting , Chen Siying , Huang Jing , Cao Chuang , Wang Yonglin , Li Yongjun , Liu Ting 
Published: September 2020

Abstract
Background: Aidi Injection (ADI), a Chinese herbal preparation with anti-cancer activity, is used for the treatment of hepatocellular carcinoma (HCC). Several clinical studies have shown that co-administration of ADI with doxorubicin (DOX) is associated with reduced toxicity of chemotherapy, enhanced clinical efficacy and improved quality of life for patients. However, limited information is available about the herb-drug interactions between ADI and DOX. The study aimed to investigate the pharmacokinetic mechanism of herb-drug interactions between ADI and DOX in a rat model of HCC.Methods: Experimental HCC was induced in rats by oral administration of diethylnitrosamine. The HCC rats were pretreated with ADI (10 mL/kg, intraperitoneal injection) for 14 consecutive days prior to administration of DOX (7 mg/kg, intravenous injection) to investigate pharmacokinetic interactions. Plasma concentrations of DOX and its major metabolite, doxorubicinol (DOXol), were determined using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Results: Preadministration of ADI significantly altered the pharmacokinetics of DOX in HCC rats, leading to increased plasma concentrations of both DOX and DOXol. The area under the plasma drug concentration-time curve (AUCs) of DOX and DOXol in rats pretreated with ADI were 3.79-fold and 2.92-fold higher, respectively, than those in control rats that did not receive ADI. Conclusions: Increased levels of DOX and DOXol were found in the plasma of HCC rats pretreated with ADI.