bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lyme neuroborreliosis in Swedish children—PCR as a complementary diagnostic method for detection of Borrelia burgdorferi sensu lato in cerebrospinal fluid
Authors: Skogman Barbro H. , Wilhelmsson Peter , Atallah Stephanie , Petersson Ann-Cathrine , Ornstein Katarina , Lindgren Per-Eric 
Published: January 2021

AbstractThe aim of this study was to evaluate polymerase chain reaction (PCR) as a diagnostic method for the detection of Borrelia burgdorferi s.l. in CSF of Swedish children with LNB. This study was performed retrospectively on CSF and serum samples collected from children evaluated for LNB (n = 233) and controls with other specific neurological disorders (n = 59) in a Swedish Lyme endemic area. For anti-Borrelia antibody index, the IDEIA Lyme Neuroborreliosis kit (Oxoid) was used. Two in-house real-time PCR assays targeting the 16S rRNA gene were evaluated (TaqMan® and LUX™). Among patients classified as LNB cases (n = 102), five children (5%) were Borrelia PCR-positive in CSF with the TaqMan® assay. In the Non-LNB group (n = 131), one patient was Borrelia PCR positive with the TaqMan® assay. Among controls (n = 59), all CSF samples were PCR negative. When amplifying and sequencing ospA, we found B. garinii (n = 2), B. afzelii (n = 2), B. bavariensis (n = 1), and one untypable (n = 1). With the LUX™ technology, all CSF samples were PCR negative. The TaqMan® assay could detect only few cases (n = 6) of B. burgdorferi s.l. in CSF among children with LNB and the sensitivity was very low (5%). However, using larger CSF volumes and centrifugation of samples, the PCR technique could still be useful as a complementary diagnostic method when evaluating LNB. Furthermore, detection of spirochete DNA in clinical matrices, including CSF, is the method of choice for studying epidemiological aspects of LNB, a tick-borne emerging disease.
Housing Instability and HIV Risk: Expanding our Understanding of the Impact of Eviction and Other Landlord-Related Forced Moves
Authors: Groves Allison K. , Niccolai Linda M. , Keene Danya E. , Rosenberg Alana , Schlesinger Penelope , Blankenship Kim M. 
Published: January 2021

AbstractThe study purpose is to comprehensively measure landlord-related forced moves (inclusive of, but not restricted to, legal eviction), and to examine whether landlord-related forced moves is associated with HIV risk. Baseline survey data was collected between 2017 and 2018 among 360 low-income participants in New Haven, Connecticut. We used multivariable logistic regression analyses to examine associations between landlord-related forced moves and HIV sexual risk outcomes. Seventy seven out of three hundred and sixty participants reported a landlord-related forced move in the past 2 years, of whom 19% reported formal eviction, 56% reported informal eviction and 25% reported both. Landlord-related forced moves were associated with higher odds of unprotected sex (AOR 1.98), concurrent sex (AOR 1.94), selling sex for money or drugs (AOR 3.28), exchange of sex for a place to live (AOR 3.29), and an HIV sexual risk composite (ARR 1.46) (p < .05 for all). We found robust associations between landlord-related forced moves and HIV sexual risk. Findings suggest that the social and economic consequences of landlord-related forced moves may impact sexual vulnerability.
A low-power 10-bit 0.01-to-12-MS/s asynchronous SAR ADC in 65-nm CMOS
Authors: Campos Arthur Lombardi , Navarro João , Luppe Maximiliam , de Lima Eduardo Rodrigues 
Published: January 2021

AbstractDuring the last decades we have witnessed the performance improvement and the aggressive growth of the complexity of integrated circuits (ICs). The progressive size reduction of transistors in recent technological nodes has allowed and even compelled IC designers to perform analog tasks in the digital domain, increasing the demand for analog-to-digital converters (ADCs). This work presents the design and implementation of a low power, differential, asynchronous successive approximation register analog-to-digital converter (SAR ADC) in a 65-nm CMOS technology. The ADC works in a flexible range of sampling rates, from a few kS/s up to 12.0 MS/s, being suitable for application in a wide spectrum of low power systems and subsystems, such as biosignal recorder interfaces and frontend of wireless receivers. At maximum sampling rate, the post-layout simulated circuit achieved an effective number of bits (ENOB) of 9.65 and a power consumption of 151.4 µW, leading to a Figure of Merit of 15.8 fJ/Conversion-step; at 10.0 kS/s sampling rate, the ENOB is almost the same, 9.63, but the power consumption is reduced to only 0.26 µW. The occupied area of the implemented ADC is 0.074 mm2.
Description and genomic characterization of Streptococcus symci sp. nov., isolated from a child’s oropharynx
Authors: Qi He , Liu Defeng , Zou Yang , Wang Nan , Tian Han , Xiao Chunling 
Published: January 2021

AbstractUsing the culturomics approach, we isolated a new Streptococcus species, strain C17T, from the oropharynx mucosa sample of a healthy 5-year-old child living in Shenyang, China. We studied the phenotypic, phylogenetic, and genomic characteristics of strain C17T, which was identified as a Gram-positive, coccus-shaped, non-motile, aerobic, catalase-negative bacteria. Its growth temperatures ranged from 20 to 42 °C, with optimal growth at 37 °C. Acid production could be inhibited by two sugars, trehalose and raffinose. In C17T, the reactions for enzyme lipase (C14) were confirmed to be negative, whereas those for alkaline phosphatase, α-glucosidase, and hippuric acid hydrolysis were positive. The C17T genome contained 2,189,419 base pairs (bp), with an average G+C content of 39.95%, encoding 2092 genes in total. The 16S ribosomal RNA sequence showed 99.8% similarity with the newly identified Streptococcus pseudopneumoniae ATCC BAA-960T. The main fatty acid components in C17T were C16:0, C18:1 w7c, C18:0, and C18:1 w9c, all of which can be found in other species of the Streptococcus genus. Strain C17T showed high susceptibility to clindamycin, linezolid, vancomycin, chloramphenicol, and cefepime, and moderate susceptibility to erythromycin. The obtained dDDH value between strain C17T and the closest species was 52.9%. In addition, the whole genome sequence of strain C17T had an 82.21–93.40% average nucleotide identity (ANI) with those strains of closely related Streptococcus species, indicating that the strain C17T was unique among all Streptococcus species. Based on these characteristics, we determine that C17T is a novel species, named Streptococcus symci sp. nov. (= GDMCC 1.1633 = JCM 33582).
Live blog summarization
Authors: Avinesh P. V. S. , Peyrard Maxime , Meyer Christian M. 
Published: January 2021

AbstractLive blogs are an increasingly popular news format to cover breaking news and live events in online journalism. Online news websites around the world are using this medium to give their readers a minute by minute update on an event. Good summaries enhance the value of the live blogs for a reader, but are often not available. In this article, (a) we first define the task of summarizing a live blog, (b) study ways of automatically collecting corpora for live blog summarization, and (c) understand the complexity of the task by empirically evaluating well-known state-of-the-art unsupervised and supervised summarization systems on our new corpus. We show that live blog summarization poses new challenges in the field of news summarization, since frequency and positional signals cannot be used. We make our tools publicly available to reconstruct the corpus and to conduct our empirical experiments. This encourages the research community to build upon and replicate our results.
Polyhedral approximations of the semidefinite cone and their application
Authors: Wang Yuzhu , Tanaka Akihiro , Yoshise Akiko 
Published: January 2021

AbstractWe develop techniques to construct a series of sparse polyhedral approximations of the semidefinite cone. Motivated by the semidefinite (SD) bases proposed by Tanaka and Yoshise (Ann Oper Res 265:155–182, 2018),
we propose a simple expansion of SD bases so as to keep the sparsity of the matrices composing it. We prove that the polyhedral approximation using our expanded SD bases contains the set of all diagonally dominant matrices and is contained in the set of all scaled diagonally dominant matrices. We also prove that the set of all scaled diagonally dominant matrices can be expressed using an infinite number of expanded SD bases. We use our approximations as the initial approximation in cutting plane methods for solving a semidefinite relaxation of the maximum stable set problem. It is found that the proposed methods with expanded SD bases are significantly more efficient than methods using other existing approximations or solving semidefinite relaxation problems directly.
Whole genome characterization of strains belonging to the Ralstonia solanacearum species complex and in silico analysis of TaqMan assays for detection in this heterogenous species complex
Authors: Kurm Viola , Houwers Ilse , Coipan Claudia E. , Bonants Peter , Waalwijk Cees , van der Lee Theo , Brankovics Balázs , van der Wolf Jan 
Published: January 2021

AbstractIdentification and classification of members of theRalstonia solanacearumspecies complex (RSSC) is challenging due to the heterogeneity of this complex. Whole genome sequence data of 225 strains were used to classify strains based on average nucleotide identity (ANI) and multilocus sequence analysis (MLSA). Based on the ANI score (>95%), 191 out of 192(99.5%) RSSC strains could be grouped into the three speciesR. solanacearum, R. pseudosolanacearum, andR. syzygii, and into the four phylotypes within the RSSC (I,II, III, and IV).R. solanacearumphylotype II could be split in two groups (IIA and IIB), from which IIB clustered in three subgroups (IIBa, IIBb and IIBc). This division by ANI was in accordance with MLSA. The IIB subgroups found by ANI and MLSA also differed in the number of SNPs in the primer and probe sites of various assays. Anin-silicoanalysis of eight TaqMan and 11 conventional PCR assays was performed using the whole genome sequences. Based on this analysis several cases of potential false positives or false negatives can be expected upon the use of these assays for their intended target organisms. Two TaqMan assays and two PCR assays targeting the 16S rDNA sequence should be able to detect all phylotypes of the RSSC. We conclude that the increasing availability of whole genome sequences is not only useful for classification of strains, but also shows potential for selection and evaluation of clade specific nucleic acid-based amplification methods within the RSSC.
Brief Report: Impact of COVID-19 on Individuals with ASD and Their Caregivers: A Perspective from the SPARK Cohort
Authors: White L. Casey , Law J. Kiely , Daniels Amy M. , Toroney Jaimie , Vernoia Brianna , Xiao Sabrina ,   The SPARK Consortium, Feliciano Pamela , Chung Wendy K. 
Published: January 2021

AbstractThe impact of the 2019 coronavirus pandemic (COVID-19) in the United States is unprecedented, with unknown implications for the autism community. We surveyed 3502 parents/caregivers of individuals with an autism spectrum disorder (ASD) enrolled in Simons Powering Autism Research for Knowledge (SPARK) and found that most individuals with ASD experienced significant, ongoing disruptions to therapies. While some services were adapted to telehealth format, most participants were not receiving such services at follow-up, and those who were reported minimal benefit. Children under age five had the most severely disrupted services and lowest reported benefit of telehealth adaptation. Caregivers also reported worsening ASD symptoms and moderate family distress. Strategies to support the ASD community should be immediately developed and implemented.
Reliability and longevity of implantable defibrillators
Authors: Hauser Robert G. , Casey Susan A. , Gitter Christopher B. , Tang Chuen Y. , Abdelhadi Raed H. , Gornick Charles C. , Stanberry Larissa , Sengupta Jay D. 
Published: January 2021

Abstract
Purpose
We hypothesized that data in manufacturers’ product performance reports (PPRs) can provide clinically valuable ICD and cardiac resynchronization defibrillator (CRT-D) reliability and longevity information.

Methods
Data were obtained from 2019 PPRs. Kaplan-Meier (K-M) probabilities of freedom from malfunction, normal battery depletion (NBD), and NBD + malfunction were calculated for ICD and CRT-D pulse generators (PGs) with LiMnO2 or LiSVO/CFx batteries marketed in the USA from 2010 to 2019 and compared using the log-rank test. Malfunctions (MAL) included PGs that were found outside specifications.

Results
Study population included 1,149,803 ICD and CRT-D PGs: Abbott (ABT; 35.1%), Biotronik (BIO; 4.6%), Boston Scientific (BSC; 23.5%), and Medtronic (MDT; 36.9%). Significant differences in reliability (p < 0.001), defined by freedom from MAL, were found between manufacturers; the majority of 6808 MAL occurred in ABT devices (n = 4045; 59.4%), followed by BSC (n = 2384; 35.0%), MDT (n = 338;5.0%), and BIO (n = 41; 0.6%). Battery failure (n = 890; 57.9%) was the most common cause of MAL compromising therapy; analysis of unique ABT battery MAL–indicated problem appeared a year prior to advisory. Significant differences (p < 0.001) in battery longevity, as defined by freedom from NBD, were found between manufacturers. Overall performance (freedom from NBD + MAL) favored BSC for CRT-D PGs and MDT and BIO for ICDs. BSC subcutaneous ICD reliability was inferior to its transvenous ICD (p < 0.001).

Conclusion
PPRs contain valuable data that can be aggregated and analyzed to inform physicians. Differences in product reliability exist between manufacturers. Battery longevity has improved, but MAL have significantly impacted performance. PPR data may be useful for assessing product problems and new technology.
Augmented Ultrasonic Data for Machine Learning
Authors: Virkkunen Iikka , Koskinen Tuomas , Jessen-Juhler Oskari , Rinta-aho Jari 
Published: January 2021

AbstractFlaw detection in non-destructive testing, especially for complex signals like ultrasonic data, has thus far relied heavily on the expertise and judgement of trained human inspectors. While automated systems have been used for a long time, these have mostly been limited to using simple decision automation, such as signal amplitude threshold. The recent advances in various machine learning algorithms have solved many similarly difficult classification problems, that have previously been considered intractable. For non-destructive testing, encouraging results have already been reported in the open literature, but the use of machine learning is still very limited in NDT applications in the field. Key issue hindering their use, is the limited availability of representative flawed data-sets to be used for training. In the present paper, we develop modern, deep convolutional network to detect flaws from phased-array ultrasonic data. We make extensive use of data augmentation to enhance the initially limited raw data and to aid learning. The data augmentation utilizes virtual flaws—a technique, that has successfully been used in training human inspectors and is soon to be used in nuclear inspection qualification. The results from the machine learning classifier are compared to human performance. We show, that using sophisticated data augmentation, modern deep learning networks can be trained to achieve human-level performance.
Microglia induce neurogenic protein expression in primary cortical cells by stimulating PI3K/AKT intracellular signaling in vitro
Authors: Lorenzen Kristi , Mathy Nicholas W. , Whiteford Erin R. , Eischeid Alex , Chen Jing , Behrens Matthew , Chen Xian-Ming , Shibata Annemarie 
Published: January 2021

AbstractEmerging evidence suggests that microglia can support neurogenesis. Little is known about the mechanisms by which microglia regulate the cortical environment and stimulate cortical neurogenesis. We used an in vitro co-culture model system to investigate the hypothesis that microglia respond to soluble signals from cortical cells, particularly following mechanical injury, to alter the cortical environment and promote cortical cell proliferation, differentiation, and survival. Analyses of cortical cell proliferation, cell death, neurogenic protein expression, and intracellular signaling were performed on uninjured and injured cortical cells in co-culture with microglial cell lines. Microglia soluble cues enhanced cortical cell viability and proliferation cortical cells. Co-culture of injured cortical cells with microglia significantly reduced cell death of cortical cells. Microglial co-culture significantly increased Nestin + and α-internexin + cortical cells. Multiplex ELISA and RT-PCR showed decreased pro-inflammatory cytokine production by microglia co-cultured with injured cortical cells. Inhibition of AKT phosphorylation in cortical cells blocked microglial-enhanced cortical cell viability and expression of neurogenic markers in vitro. This in vitro model system allows for assessment of the effect of microglial-derived soluble signals on cortical cell viability, proliferation, and stages of differentiation during homeostasis or following mechanical injury. These data suggest that microglia cells can downregulate inflammatory cytokine production following activation by mechanical injury to enhance proliferation of new cells capable of neurogenesis via activation of AKT intracellular signaling. Increasing our understanding of the mechanisms that drive microglial-enhanced cortical neurogenesis during homeostasis and following injury in vitro will provide useful information for future primary cell and in vivo studies.
GIS-based identification and visualization of multimodal freight transportation catchment areas
Authors: Asborno Magdalena I. , Hernandez Sarah , Yves Manzi 
Published: January 2021

AbstractTo estimate impacts, support cost–benefit analyses, and enable project prioritization, it is necessary to identify the area of influence of a transportation infrastructure project. For freight related projects, like ports, state-of-the-practice methods to estimate such areas ignore complex interactions among multimodal supply chains and can be improved by examining the multimodal trips made to and from the facility. While travel demand models estimate multimodal trips, they may not contain robust depictions of water and rail, and do not provide direct observation. Project-specific data including local traffic counts and surveys can be expensive and subjective. This work develops a systematic, objective methodology to identify multimodal “freight-shed” (or “catchment” areas) for a facility from vehicle tracking data and demonstrates application with a case study involving diverse freight port terminals. Observed truck Global Positioning System and maritime Automatic Identification System data are subjected to robust pre-processing algorithms to handle noise, cluster stops, assign data points to the network (map-matching), and address spatial and temporal conflation. The method is applied to 43 port terminals on the Arkansas River to estimate vehicle miles and hours travelled, origin, destination, and pass-through zones, and areas of modal overlap within the catchment areas. Case studies show that the state-of-the-practice 100-mile diameter influence areas include between 15 and 34% of the multimodal freight-shed areas mined from vehicle tracking data, demonstrating that adoption of an arbitrary radial area for different ports would lead to inaccurate estimates of project benefits.
Occurence of Post-Traumatic Stress Symptoms, Anxiety and Depression in the Acute Phase of Transient Ischemic Attack and Stroke
Authors: Müller Helge H. O. , Czwalinna Katharina , Wang Ruihao , Lücke Caroline , Lam Alexandra P. , Philipsen Alexandra , Gschossmann Jürgen M. , Moeller Sebastian 
Published: January 2021

AbstractRates of post-traumatic stress symptoms, anxiety and depression are increased in patients having experienced a transient ischemic attack (TIA) or stroke several months ago. However, data of psychiatric symptoms in the acute phase within the first days after ictus are lacking. In 20 patients with stroke and 33 patients with TIA we assessed disease severity by means of the NIHSS, levels of depression and anxiety by HADS, PTSD-like symptoms by PC-PTSD, quality of life (HrQoL) by SF-12, and coping style by brief COPE Inventory within the first 5 days after ictus. NIHSS on admission was lower in patients with TIA (0 ± 1) than in patients with stroke (3 ± 2, p < 0.001). HADS depression score was significantly higher in patients with stroke (7.0 ± 4.5) than in patients with TIA (4.9 ± 4.0). HADS anxiety score, HrQoL and coping styles were similar between TIA and stroke patients (p > 0.05). 5 and 3 of 33 TIA patients as well as 4 and 3 of 20 stroke patients had at least 11 points in the HADS anxiety and depression score respectively (p = 0.001). 2 of 33 TIA patients and 2 of 20 stroke patients had more than 2 points in the PC-PTSD (p = 0.646). We did not find consistent correlations between the NIHSS and the psychometric parameters. Within the first five days after patients having experienced a TIA or stroke PTSD-like, anxious and depressive symptoms are more common than in the general population. As the acute psychological status after ictus is predictive for psychiatric comorbidity years later physicians should pay attention and adequately treat psychiatric symptoms already in the acute phase of stroke.Trial Registration: German Clinical Trials Register, DRKS00021730, https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00021730, registered 05/19/2020- Retrospectively registered.
A meta-analysis of traditional and functional end-to-side anastomosis in radiocephalic fistula for dialysis access
Authors: Weigang Tang , Wei Xu , Lifeng Gong , Jingkui Lu , Yani Li , Huaqin Jiang , Hui Li 
Published: January 2021

Abstract
Objective
Functional vein end to arterial side (ETS) anastomosis uses vein side to arterial side (STS) anastomosis with distal vein ligation, which can achieve similar effects as those of ETS after STS anastomosis. The purpose of the study was to provide a meta-analysis to compare the clinical outcomes between traditional and functional ETS anastomosis in radiocephalic fistula for dialysis access.

Methods
Databases including PubMed, EMbase, the Cochrane Library, CNKI, Wanfang database were searched from the inception to February 6, 2020. Eligible studies comparing traditional and functional ETS anastomosis in radiocephalic fistula were included. Data were analyzed using Review Manager Version 5.3.

Results
Seven studies were included in the meta-analysis. Five randomized controlled trials and two cohort studies involving 841 patients were identified. Compared with traditional ETS anastomosis, functional ETS anastomosis had shorter anastomosis time (MD − 9.54, 95% CI − 17.96 to − 1.12, P = 0.03), higher surgical success rate (OR 3.80, 95% CI 1.76–8.22, P < 0.01), fewer complications(OR 0.18, 95% CI 0.08–0.39, P < 0.01), higher patency rate after 3 months (OR 4.91, 95% CI 1.19–20.33, P = 0.03), higher patency rate after 6 months (OR 1.90, 95%CI 1.09–3.31, P = 0.02), higher patency rate after 12 months (OR 1.70, 95% CI 1.09–2.66, P = 0.02). There was no difference after the two arteriovenous (AVF) anastomosisl methods concerning AVF maturation time (SMD − 0.48, 95% CI − 1.30–0.34, P = 0.25) and patency rate after 1 month (OR 1.77, 95% CI 0.65–4.80, P = 0.26).

Conclusion
Functional ETS anastomosis had advantages of easy operation, high surgical success rate, few complications, high patency rate of 3 months and long-term, but did not have obvious advantage in the early stages concerning AVF maturation time and 1-month patency rate.