bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of the “Health Transformation Plan” and related policies on the prevalence rate of cesarean section in Iran: Insights from an interrupted time series analysis
Authors: Behzadifar Masoud , Behzadifar Meysam , Saki Mandana , Valipour Mehrdad , Omidifar Roodabeh , Iranshahi Fatemeh , Bragazzi Nicola Luigi 
Published: October 2019

SummaryBackgroundThe high prevalence of cesarean section represents a major public health challenge worldwide. In 2014, the Iranian Health Transformation Plan (HTP) included programs promoting vaginal delivery.AimThe aim of this study was to investigate the effect of the HTP on the rate of cesarean section in Iran.MethodThe interrupted time series analysis (ITSA) was used. Cesarean section– and vaginal delivery–related monthly data were collected from eight public hospitals affiliated with the Lorestan University of Medical Sciences, from March 2012 to February 2019. The autocorrelation plots and the Durbin‐Watson test were used for evaluating the autocorrelation between data points in the generalized least squares regression model.ResultsThe ITSA showed that the rate of cesarean section decreased immediately after the HTP, by −0.002 per 1000 persons (95% CI, −0.004 to −0.001; P = .069). After the HTP, a significant decreasing trend of cesarean section per month was computed (−0.003; 95% CI, −0.005 to 0.012; P = .043).ConclusionThe present study showed that the implementation of the HTP policy was effective in reducing the rate of cesarean section. This policy should continue, involving relevant stakeholders, raising mothers' awareness and motivation, and providing financial support.
A paleo‐hydro‐geomorphological perspective on urban flood risk assessment
Authors: Link Oscar , Brox‐Escudero Luis M. , González John , Aguayo Mauricio , Torrejón Fernando , Montalva Gonzalo , Eguibar‐Galán Miguel Á. 
Published: October 2019

AbstractComprehensive flood risk assessment requires enhanced understanding of the coevolution of the river and its floodplain occupation. Paleoflood analysis to determine flood prone areas in combination with numerical simulations to estimate flood hazard and a historical analysis of urban development to consider the evolution of exposure to floods is a possible way forward. The well‐documented 2006 extreme flood in the Biobío River system and the impacted metropolitan area of Concepción, Chile (~1 million inhabitants) was used as a complex scenario to test the reliability of the proposed method. Results showed that flood prone areas determined with hydro‐geomorphological methods are consistent with those computed with numerical models based on detailed digital elevation models. The flood generation via superficial flow pathways resulting in inundated areas could explain that rivers tend to reactivate paleochannels in extreme conditions. Urban development progressively increased the city's exposure to floods from 0 ha in 1,751 to 1,363 ha in 2006 evidencing a lack of appropriate flood risk management. The 100‐year peak discharge resulted in a high flood risk for about 5% of the total urbanized area of Concepción, and higher discharges are likely to reactivate a paleochannel that crosses the current city centre. We conclude that the proposed paleo hydro‐geomorphology, hydraulic, and urban planning multimethod approach is a necessary tool to enhance understanding of flood risk in complex scenarios to improve flood risk management.
Mortalin contributes to colorectal cancer by promoting proliferation and epithelial–mesenchymal transition
Authors: Xu Ming , Zhang Yuan , Cui Minghua , Wang Xinyue , Lin Zhenhua 
Published: October 2019

AbstractThis study focused on the expression of mortalin in colorectal cancer (CRC). Mortalin activated the Wnt/β‐catenin pathway to accelerate cell proliferation and the epithelial–mesenchymal transition (EMT) program. Data from online databases displayed that the expression of mortalin was high in CRC, which was further validated using clinical specimens. Meanwhile, high mortalin expression was positively associated with a poor overall survival rate. Suppression of mortalin inhibited CRC cell proliferation as evaluated by the 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide (MTT), colony formation, and immunofluorescence staining assays. In addition, depletion of mortalin inhibited CRC cell EMT progression and deactivated the Wnt/β‐catenin pathway. Altogether, mortalin is highly expressed in CRC and may indicate a poor prognosis. Mortalin accelerated CRC progression by stimulating cell proliferation and the EMT program. This study may provide a potential clinical therapeutic target for CRC.
LncRNA AC009022.1 enhances colorectal cancer cells proliferation, migration, and invasion by promoting ACTR3B expression via suppressing miR‐497‐5p
Authors: Yu Chen , Zhang Fengchun 
Published: October 2019

AbstractPurposeLong noncoding RNAs (lncRNAs) play an indispensable role in cancer progression. We aim at exploring the effect of AC009022.1 on colorectal cancer (CRC) development in this paper.MethodsCRC tissues and matched normal tissues of 68 CRC patients were collected. HCT‐116 and SW620 cells were transfected and grouped. The cell counting kit‐8 assay, cell scratch test, and Transwell experiment were performed to sequentially detect cells proliferation, migration, and invasion. In vivo experiments were conducted. The Luciferase reporter gene assay was used. Gene expression was detected by quantitative reverse transcription polymerase chain reaction and Western blot analysis.ResultsAC009022.1 expression was abnormally elevated in CRC tissues and cells. High AC009022.1 expression in CRC patients was significantly associated with poor prognosis. Compared with the siNC group, HCT‐116 and SW620 cells of siAC009022.1 group exhibited much lower OD450 value (P < .001) and invasive cell number (P < .01), and significantly higher relative wound width (P < .01). Much lower subcutaneous tumor volume and weight were found in the siAC009022.1 group compared with siNC group (P < .001). In CRC cells, AC009022.1 directly inhibited miR‐497‐5p expression while miR‐497‐5p directly hindered ACTR3B expression. Compared with HCT‐116 and SW620 cells of oe‐AC009022.1 group, those of oe‐AC009022.1 + miR‐497‐5p‐mimics group and oe‐AC009022.1 + siACTR3B group had obviously lower OD450 value (P < .001) and invasion cell number (P < .01), and markedly higher relative wound width (P < .01).ConclusionsAC009022.1 enhanced CRC cell proliferation, migration, and invasion by promoting ACTR3B expression via suppressing miR‐497‐5p.
A seven‐DNA methylation signature as a novel prognostic biomarker in breast cancer
Authors: Tao Chuntao , Luo Ruihan , Song Jing , Zhang Wanfeng , Ran Longke 
Published: October 2019

AbstractBackgroundBreast cancer (BC) is a common malignant tumor and its incidence and mortality rates are ranked first among female cancers. So far, there has been no effective biomarkers for BC prognosis.MethodsThe DNA methylation data of BC was downloaded from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus, and Functional ANnoTation of The Mammalian Genome databases. The RNA‐Seq data and clinical information of patients were downloaded from TCGA. R packages edgeR and minfi were used for differentially methylated genes (DMGs) screening. Then, the DMGs were collected for gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis by the online tool database for annotation, visualization and integrated discovery (DAVID) and Reactome. Cox regression analysis was used to screen candidate differentially methylated sites (DMSs) for BC prognosis. Logrank test was used to explore the correlation between DMSs and survival time. Correlation analysis was used to investigate the correlation between DNA methylation and gene expression.ResultsWe identified 276 DMGs which contained 1454 DMSs in those three datasets. Also, six DMGs that contained seven DMSs were identified by Cox regression analysis. Interestingly, their expression levels were negatively correlated with the DNA methylation level and not affected by age, subtypes, or tumor stages.ConclusionsWe proposed that these seven differentially DNA methylation sites can be used as a novel prognostic biomarker for BC area under curve (AUC) = 0.74), which may facilitate research and benefit the clinical treatment of BC.
Sense of community, psychological empowerment, and relational structure at the individual and organizational levels: Evidence from a multicase study
Authors: Ramos‐Vidal Ignacio , Palacio Jorge , Uribe Alicia , Villamil Ilse , Castro Belkis 
Published: October 2019

AbstractThe aim of this study was to determine the associations among psychological sense of community (PSoC), psychological empowerment (PE), and relational structure with indicators of centrality and cohesion in egocentric and sociocentric networks when analyzed at different levels. A multicase analysis was used (four developed in Colombia and one in Spain) in which 458 people participated in five studies conducted in organizational and community contexts of vulnerable urban and rural areas. The results suggest that PSoC and PE are intertwined processes whose association is accentuated in organizational contexts. However, the relationships between relational context and PSoC and PE vary notably depending on (a) the type of relationship evaluated, (b) the focus of the study (individual/egocentric vs. group/sociocentric) and the type of indicator used to assess the relational context (centrality vs. structural cohesion). Findings are discussed to understand the role that social bonds play in the development of PSoC and in the ability of subjects to empower themselves in different contexts.
Sense of belonging to the community in continuing care retirement communities and adult day care centers: The role of the social network
Authors: Ayalon Liat 
Published: October 2019

AbstractAimThis study examined the contribution of the social network to one's sense of belonging to the community (SCB) in two different long‐term care settings: Continuing care retirement communities (CCRCs) and adult day care centers (ADCCs).MethodsOverall, 245 respondents participated in both waves of the study that were spaced about 1 year apart.ResultsResults show that constraint in the social network (e.g., being socially invested in a single group of interconnected ties) is particularly detrimental for CCRC residents, but not for ADCC participants. Betweenness, defined as the number of shortest paths that pass through the focal person, was not significantly related to SCB. In addition, the size of the egocentric network, obtained through a name generator of a list of all potentially meaningful individuals identified by respondents, was directly correlated with SCB at follow‐up.ConclusionsThe findings are discussed in light of differences between the two long‐term care settings and the different social network indicators examined in this study.
Retracted: Circular RNA circFNDC3B protects renal carcinoma by miR‐99a downregulation
Authors: Chen Tao , Yu Qinchao , Shao Shixiu , Guo Lei 
Published: October 2019

AbstractVarious circular RNAs (circRNAs) have been reported to involve in carcinoma. This study explored the role and mechanism of circRNA circFNDC3B (circFNDC3B) in renal carcinoma (RC). The detection indicators in this paper were viability, colony, and migration, which respectively investigated by Cell Counting Kit‐8, colony formation, and migration assay. Reverse transcriptase quantitative polymerase chain reaction tested and cell transfection changed circFNDC3B and miR‐99a expression. Moreover, western blot tested relate‐proteins of proliferation, migration, and cell pathways were examined by western blot. circFNDC3B was upregulated at RC tissues. circFNDC3B enhanced cell viability, colony and migration, and miR‐99a mimic played reverse impacts. Furthermore, circFNDC3B negatively regulated miR‐99aand circFNDC3B restrained the janus kinase 1/signal transducer and activator of transcription 3 (JAK1/STAT3) and extracellular signal‐regulated kinase (ERK) kinase (MEK)/ERK pathways by miR‐99a downregulation. Overexpression of circFNDC3B enhanced cell viability, colony formation and migration by miR‐99a downregulation via JAK1/STAT3 and MEK/ERK pathways.
Developments in the study of gastrointestinal microbiome disorders affected by FGF19 in the occurrence and development of colorectal neoplasms
Authors: Peng Meichang , Zheng Qiaowen , Liu Peiqi , Liang Xinyun , Zhang Min , Wang Yan , Zhao Yi 
Published: October 2019

AbstractColorectal neoplasms are a type of malignant digestive system tumor that has become the third‐highest morbidity tumor in China and the fourth leading cause of cancer‐related death worldwide. The role of the gastrointestinal (GI) microbiome in bile acid metabolism, inflammation, and insulin resistance and its strong correlation with the occurrence and development of colorectal neoplasms have gradually led to it becoming a target area of tumor research. Fibroblast growth factor (FGF) 19 is a hormone that is secreted in mainly the ileum and can regulate bile acid biosynthesis, improve inflammation, and regulate insulin resistance. The relationship of the GI microbiome, FGF19 and its carcinogenic activities in colorectal neoplasms enticed us to search for potential targets and research ideas for the clinical diagnosis and treatment of colorectal neoplasms.
Extra domain A‐containing fibronectin expression in Spin90‐deficient fibroblasts mediates cancer‐stroma interaction and promotes breast cancer progression
Authors: Kwon Ahreum , Chae In Hee , You Eunae , Kim So Hee , Ahn Su‐yeon , Lee Ok‐Jun , Park Zee‐Yong , Rhee Sangmyung , Huh Yun Hyun , Song Woo Keun 
Published: October 2019

AbstractCancer‐associated fibroblasts (CAFs) in the tumor microenvironment play major roles in supporting cancer progression. A previous report showed that SPIN90 downregulation is correlated with CAF activation and that SPIN90‐deficient CAFs promote breast cancer progression. However, the mechanisms that mediate cancer‐stroma interaction and how such interactions regulate cancer progression are not well understood. Here, we show that extra domain A (EDA)‐containing fibronectin (FN), FN(+)EDA, produced by mouse embryonic fibroblasts (MEFs) derived from Spin90‐knockout (KO) mice increases their own myofibroblast differentiation, which facilitates breast cancer progression. Increased FN(+)EDA in Spin90‐KO MEFs promoted fibril formation in the extracellular matrix (ECM) and specifically interacted with integrin α4β1 as the mediating receptor. Moreover, FN(+)EDA expression by Spin90‐KO MEFs increased proliferation, migration, and invasion of breast cancer cells. Irigenin, a specific inhibitor of the interaction between integrin α4β1 and FN(+)EDA, significantly blocked the effects of FN(+)EDA, such as fibril formation by Spin90‐KO MEFs and proliferation, migration, and invasion of breast cancer cells. In orthotopic breast cancer mouse models, irigenin injection remarkably reduced tumor growth and lung metastases. It was supported by that FN(+)EDA in assembled fibrils was accumulated in cancer stroma of human breast cancer patients in which SPIN90 expression was downregulated. Our data suggest that SPIN90 downregulation increases FN(+)EDA and promotes ECM stiffening in breast cancer stroma through an assembly of long FN(+)EDA‐rich fibrils; moreover, engagement of the Integrin α4β1 receptor facilitates breast cancer progression. Inhibitory effects of irigenin on tumor growth and metastasis suggest the potential of this agent as an anticancer therapeutic.
Cell mechanical microenvironment for cell volume regulation
Authors: Wang Meng , Yang Yaowei , Han Lichun , Xu Feng , Li Fei 
Published: October 2019

AbstractCell volume regulation, as one of the fundamental homeostasis of the cell, is associated with many cellular behaviors and functions. With the increased studies on the effect of environmental mechanical cues on cell volume regulation, the relationship between cell volume regulation and mechanotransduction becomes more and more clear. In this paper, we review the mechanisms and hypotheses by which cell maintains its volume homeostasis both in vivo and in constructed cell mechanical microenvironment (CMM) in vitro. We discuss how the growth‐division regulation maintains the volume homeostasis of cells in the cell cycle and how the cell cortex/membrane tension mediates the effect of CMM (i.e., osmotic pressure, matrix stiffness, and mechanical force) on cell volume regulation. We also highlight the roles of cell volume as a perfect integrator of the downstream signals of mechanotransduction from different aspects of CMM and an effective indicator for the mechanical condition that cell confronts. This interdisciplinary perspective can provide new insight into biomechanics and may shed light on bioengineering and pathological research work. We hope this review can facilitate future studies on the investigation of the role of cell volume in mechanotransduction.
Podosome formation impairs endothelial barrier function by sequestering zonula occludens proteins
Authors: Rui Yan‐Ning , Chen Yawen , Guo Yichen , Bock Caroline E. , Hagan John P. , Kim Dong H. , Xu Zhen 
Published: October 2019

AbstractPodosomes and tight junctions (TJs) are subcellular compartments that both exist in endothelial cells and localize at cell surfaces. In contrast to the well‐characterized role of TJs in maintaining cerebrovascular integrity, the specific function of endothelial podosomes remains unknown. Intriguingly, we discovered cross‐talk between podosomes and TJs in human brain endothelial cells. Tight junction scaffold proteins ZO‐1 and ZO‐2 localize at podosomes in response to phorbol‐12‐myristate‐13‐acetate treatment. We found that both ZO proteins are essential for podosome formation and function. Rather than being derived from new protein synthesis, podosomal ZO‐1 and ZO‐2 are relocated from a pre‐existing pool found at the peripheral plasma membrane with enhanced physical interaction with cortactin, a known protein marker for podosomes. Sequestration of ZO proteins in podosomes weakens tight junction complex formation, leading to increased endothelial cell permeability. This effect can be further attenuated by podosome inhibitor PP2. Altogether, our data revealed a novel cellular function of podosomes, specifically, their ability to negatively regulate tight junction and endothelial barrier integrity, which have been linked to a variety of cerebrovascular diseases.
Identification of a prognostic alternative splicing signature in oral squamous cell carcinoma
Authors: Zhang Shuting , Wu Xiang , Diao Pengfei , Wang Chenxing , Wang Dongmiao , Li Sheng , Wang Yanling , Cheng Jie 
Published: October 2019

AbstractAlternative splicing (AS) is critically associated with tumorigenesis and patient's prognosis. Here, we systematically analyzed survival‐associated AS signatures in oral squamous cell carcinoma (OSCC) and evaluated their prognostic predictive values. Survival‐related AS events were identified by univariate and multivariate Cox regression analyses using OSCC data from the TCGA head neck squamous cell carcinoma data set. The Percent Spliced In calculated by SpliceSeq from 0 to 1 was used to quantify seven types of AS events. A predictive model based on AS events was constructed by least absolute shrinkage and selection operator Cox regression assay and further validated using a training‐testing cohort design. Patient survival was estimated using the Kaplan–Meier method and compared with Log‐rank test. The receiver operating characteristics curve area under the curves was used to evaluate the predictive abilities of these predictive models. Furthermore, gene–gene interaction networks and the splicing factors (SFs)‐AS regulatory network was generated by Cytoscape. A total of 825 survival‐related AS events within 719 genes were identified in OSCC samples. The integrative predictive model was better at predicting outcomes of patients as compared to those models built with the individual AS event. The predictive model based on three AS‐related genes also effectively predicted patients’ survival. Moreover, seven survival‐related SFs were detected in OSCC including RBM4, HNRNPD, and HNRNPC, which have been linked to tumorigenesis. The SF‐AS network revealed a significant correlation between survival‐related AS genes and these SFs. Our findings revealed a systemic portrait of survival‐associated AS events and the splicing network in OSCC, suggesting that AS events might serve as novel prognostic biomarkers and therapeutic targets for OSCC.
Synergistic catalysis of acid–base bifunctional ionic liquids for pentanal self‐condensation reaction
Authors: An Hualiang , Kong Yunhuan , Yang Qiusheng , Zhao Xinqiang , Wang Yanji 
Published: October 2019

AbstractBACKGROUNDPentanal self‐condensation to 2‐propyl‐2‐heptenal is an important process of carbon chain extension for industrial manufacture of 2‐propylheptanol. This reaction in industry employs an aqueous caustic alkali catalyst, causing several problems of equipment corrosion, treatment of alkali wastewater, etc. Herein, several acid–base bifunctional ionic liquids were synthesized for catalyzing pentanal self‐condensation.RESULTS[PEmim]Cl‐0.5Zn(CH3COO)2 exhibited a better catalytic performance, and the yield and selectively of 2‐propyl‐2‐heptenal could reach 82.0% and 86.6%, respectively. The recovered [PEmim]Cl‐0.5Zn(CH3COO)2 could be reused three times after being regenerated without a significant loss in its catalytic performance. The structures and charge properties of [PEmim]Cl‐Zn(CH3COO)2 and a complex of [PEmim]Cl‐Zn(CH3COO)2 and pentanal were simulated by density functional theory calculation. The results show that the action of acid and base sites of [PEmim]Cl‐Zn(CH3COO)2 can effectively activate pentanal by enhancing the electrophilicity or nucleophilicity of different pentanal molecules.CONCLUSIONThe acid–base synergetic effect of [PEmim]Cl‐Zn(CH3COO)2 could account for its high catalytic performance. An acid–base synergetic catalysis mechanism of [PEmim]Cl‐Zn(CH3COO)2 in pentanal self‐condensation was established. © 2019 Society of Chemical Industry
Fetal tongue posture associated with micrognathia: An ultrasound marker of cleft secondary palate?
Authors: D'Ambrosio Valentina , Vena Flaminia , Manganaro Lucia , Cascone Piero , Boccherini Chiara , Piccioni Maria G. , Pizzuti Antonio , Benedetti Panici Pierluigi , Giancotti Antonella 
Published: October 2019

AbstractCleft lip and cleft palate (CP) are the most common facial malformations. Two‐dimensional (2D) ultrasound (US) is the first‐line examination in the prenatal diagnosis of CP. Three‐dimensional, four‐dimensional US and MRI provide a better detection of facial clefts. We present two fetuses with micrognathia and suspected secondary CP on 2D US: fetal tongue appeared in an unusual position (low tip and high dorsum position) and showed uncoordinated movements. MRI did not confirm the US suspicion, but at birth the two fetuses were affected by Pierre Robin sequence.