bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of differential peptide loading on tandem mass tag-based proteomic and phosphoproteomic data quality
Authors: Sanford James A , Wang Yang , Hansen Joshua R , Gritsenko Marina A , Weitz Karl K , Tognon Cristina E , Petyuk Vladislav A , Qian Wei-Jun , Liu Tao , Druker Brian J , Rodland Karin D , Piehowski Paul D. 
Published: December 2020

Abstract
Background: Global and phosphoproteome profiling has demonstrated great utility for the analysis of clinical specimens. One major barrier to the broad clinical application of proteomic profiling is the large amount of biological material required, particularly for phosphoproteomics—currently on the order of 25 mg wet tissue weight, depending on tissue type. For hematopoietic cancers such as acute myeloid leukemia (AML), the sample requirement is in excess of 10 million (1E7) peripheral blood mononuclear cells (PBMCs). Throughout the course of a prospective study, this requirement will certainly exceed what is obtainable from many of the individual patients/timepoints. For this reason, we were interested in examining the impact of differential peptide loading across multiplex channels on proteomic data quality. Methods: To achieve this, we tested a range of channel loading amounts (20, 40, 100, 200, and 400 μg of tryptic peptides, or approximately the material obtainable from 5E5, 1E6, 2.5E6, 5E6, and 1E7 AML patient cells) to assess proteome coverage, quantification precision, and peptide/phosphopeptide detection in experiments utilizing isobaric tandem mass tag (TMT) labeling. Results: As expected, we found that fewer missing values are observed in TMT channels with higher peptide loading amounts compared to those with lower loading. Moreover, channels with lower loading amounts have greater quantitative variability than channels with higher loading amounts. Statistical analysis of the differences in means among the five loading groups showed that the 20 μg loading group was significantly different from the 400 μg loading group. However, no significant differences were detected among the 40, 100, 200 and 400 μg loading groups. Conclusions: These assessment data demonstrate the practical limits of loading differential quantities of peptides across channels in TMT multiplexes, and provide a basis for designing the optimal clinical proteomics study when specimen quantities are limited.
Cholesterol Alters Mitophagy by Impairing Optineurin Recruitment and Lysosomal Clearance in Alzheimer’s Disease
Authors: Roca-Agujetas Vicente , Barbero-Camps Elisabet , de Dios Cristina , Podlesniy Petar , Abadin Xenia , Morales Albert , Marí Montserrat , Trullàs Ramon , Colell Anna 
Published: December 2020

Abstract
Background: Emerging evidence indicates that impaired mitophagy-mediated clearance of defective mitochondria is a critical event in Alzheimer’s disease (AD) pathogenesis. Amyloid-beta (Aβ) metabolism and the microtubule-associated protein tau have been reported to regulate key components of the mitophagy machinery. However, the mechanisms that lead to mitophagy dysfunction in AD are not fully deciphered. We have previously shown that intraneuronal cholesterol accumulation can disrupt the autophagy flux, resulting in low Aβ clearance. In this study, we examine the impact of neuronal cholesterol changes on mitochondrial removal by autophagy.Methods: Regulation of PINK1-parkin-mediated mitophagy was investigated in conditions of acute (in vitro) and chronic (in vivo) high cholesterol loading using cholesterol-enriched SH-SY5Y cells, cultured primary neurons from transgenic mice overexpressing active SREBF2 (sterol regulatory element binding factor 2), and mice of increasing age that express the amyloid precursor protein with the familial Alzheimer Swedish mutation (Mo/HuAPP695swe) and mutant presenilin 1 (PS1-dE9) together with active SREBF2.Results: In cholesterol-enriched SH-SY5Y cells and cultured primary neurons, high intracellular cholesterol levels stimulated mitochondrial PINK1 accumulation and mitophagosomes formation triggered by Aβ while impairing lysosomal-mediated clearance. Antioxidant recovery of cholesterol-induced mitochondrial glutathione (GSH) depletion prevented mitophagosomes formation indicating mitochondrial ROS involvement. Interestingly, when brain cholesterol accumulated chronically in aged APP-PSEN1-SREBF2 mice the mitophagy flux was affected at the early steps of the pathway, with defective recruitment of the key autophagy receptor optineurin (OPTN). Sustained cholesterol-induced alterations in APP-PSEN1-SREBF2 mice promoted an age-dependent accumulation of OPTN into HDAC6-positive aggresomes, which disappeared after in vivo treatment with GSH ethyl ester (GSHee). The analyses in post-mortem brain tissues from individuals with AD confirmed these findings, showing OPTN in aggresome-like structures that correlated with high mitochondrial cholesterol levels in late AD stages.Conclusions: Our data demonstrate that accumulation of intracellular cholesterol reduces the clearance of defective mitochondria and suggest recovery of the cholesterol homeostasis and the mitochondrial scavenging of ROS as potential therapeutic targets for AD.
Medium-term outcomes after laparoscopic revision of laparoscopic Kasai portoenterostomy in patients with biliary atresia
Authors: Ji Yi , Zhang Xuepeng , Chen Siyuan , Li Yanan , Yang Kaiying , Zhou Jiangyuan , Xu Zhicheng 
Published: December 2020

Abstract
Objective: To determine whether revision laparoscopic Kasai portoenterostomy (RLKPE) is a viable treatment option for patients with biliary atresia (BA) who underwent initially successful laparoscopic Kasai portoenterostomy (ILKPE).Methods: The medical records of 312 patients with nonsyndromic BA who underwent ILKPE between May 2009 and May 2017 were retrospectively reviewed. The patients were divided into three groups according to their outcomes after ILKPE: group A: 25 patients who underwent RLKPE; group B: 203 patients who underwent ILKPE and required no further surgical intervention; and group C: 84 patients with failed ILKPE who either died or required liver transplantation for survival. The 3-year and 5-year survival with native liver (SNL) rates were compared between groups A and B C. Among the 25 patients in group A, the perioperative data of RLKPE were compared with those of ILKPE.Results: Of the 312 patients who underwent ILKPE, 228 reached the normal bilirubin concentration range within 6 months postoperatively. Among them, 25 patients with a sudden cessation of bile flow underwent RLKPE. Adequate biliary drainage evidenced by normalized conjugated bilirubin levels was achieved in 80% of the patients who underwent RLKPE. The perioperative variables, including operative time, blood loss, rate of conversion to open surgery and complications of RLKPE, were not significantly different between RLKPE and ILKPE. The 3-year and 5-year SNL rates in patients after RLKPE were 64.0% and 52.0%, respectively, which were not significantly different from the corresponding 86.2% and 73.9% in patients after unrevised ILKPE (P>0.05).Conclusion: Our data demonstrated that RPLKE can be a viable and effective treatment opinion in patients who experience sudden cessation of bile drainage after ILKPE. RPLKE can delay the need for liver transplantation, yielding encouraging medium-term patient outcomes.
TANTANGAN PEREMPUAN DITENGAH FAHAM FEMINISME PADA KOMUNITAS PEREMPUAN BERDAULAT DI MOJOKERTO
Authors: Hasanah Mizanul 
Published: December 2020

Abstrak: Komunitas perempuan berdaulat adalah komunitas yang lahir atas pemikiran kaum muda khususnya perempuan yang ingin berdaya baik secara pemikiran maupun solutif dari segi pemikiran. Apalagi ditengah-tengah pertentangan faham feminisme yang penuh pro dan kontra. Namun pada komunitas ini memberikan informasi dan solusi tersendiri tentang bagaimana bersikap dan menempatkan diri sebagai perempuan, ibu, anak, dan anggota masyarakat. Ciri khas dari perempuan berdaulat ini adalah dengan adanya moto Perempuan berdaulat Indonesia hebat. “Cerdas yang mecerdaskan hebat menghebatkan”. Penelitian ini dilakukan di kabupaten Mojokerto kecamatan Pacet Provinsi Jawa Timur yang mana komunitas ini dicetuskan oleh mahasiswi Institut KH. Abdul Chalim Mojokerto. Tujuan dari komunitas ini adalah agar perempuan berdaulat secara pemikiran dan berdaya di segala bidang di masa mendatang. Adanya komunitas ini selain memotivasi, mengantisipasi, adalah memberikan edukasi kepada orang lain, memberi stimulus dengan menggali ilmu yang mendalam sehingga mereka mampu memberi solusi yang ilmiah dan mampu diterima dalam masyarakat. Kata kunci: Perempuan, Faham feminisme, Komunitas Perempuan Berdaulat Abstract: Sovereign women's community is a community that was born of the minds of young people, especially women who want to be empowered both in thought and solutive in terms of thinking. Especially amidst the contradictions of feminism that are full of pros and cons. However, this community provides its own information and solutions on how to behave and position themselves as women, mothers, children and community members. The distinctive feature of this sovereign woman is the motto of a great Indonesian sovereign woman. "Smart, smart, great, great". This research was conducted in Mojokerto district, Pacet sub-district, East Java Province where this community was initiated by a student of the KH Institute Abdul Chalim Mojokerto. The aim of this community is for women to be sovereign in their thinking and empowered in all fields in the future. The existence of this community in addition to motivating, anticipating, is providing education to others, providing stimulus by exploring in-depth knowledge so that they are able to provide scientific solutions and are able to be accepted in society. Keywords: Keywords: Women, Feminism, Sovereign Women's Community
DISMILARITAS GENDER PADA KOGNITIF DAN SOSIAL-EMOSIONAL ANAK USIA DINI
Authors: Rukmana Ika Siti 
Published: December 2020

Abstrak: Tujuan dari penelitian ini adalah untuk mengidentifikasi dismilaritas anak lelaki dan anak perempuan pada kognitif dan sosial emosionalnya. Metodenya menggunakan library research. pengumpulan data dari jurnal, buku, dll. Hasil penelitian ini menunjukkan bahwa dismilaritas gender pada kognitif anak usia dini adalah anak lelaki lebih unggul dalam matematika dan visualspasial. Sedangkan. dismilaritas gender pada sosial-emosional anak usia dini, yakni anak perempuan lebih dalam membaca dan writing, anak lelaki kurang mengikuti nuraninya, anak lelaki lebih agresif fisiknya, anak perempuan lebih bisa mempengaruhi dalam bergosip, anak perempuan lebih bisa kontrol diri, anak lelaki lebih sering mendapat masalah dari eksternalnya, dan anak perempuan lebih memperhatikan guru di kelas. Terdapat strategi mendidik anak lelaki, yaitu ajaklah anak lelaki agar lebih sensitif, mengurangi keagresifan fisiknya, dan tingkatkanlah prestasi di sekolah. Adapun strategi dalam mendidik anak perempuan, yaitu apresiasilah dan dukunglah anak perempuan dalam kepekaannya dan pengasuhan, serta ajaklah anak perempuan agar lebih aktif dalam mengungkapkan aspirasinya.

Kata kunci: Dismilaritas, gender, kognitif, sosial-emosional, anak

Abstract: T The aim of this study is to identify boys and girls in cognitive and social emotional. The method uses library research. data from journals, books, etc. The results showed that gender dysmillarity in early childhood cognitive was that boys were superior in mathematics and visual spatial. While. gender dysmillarity in the socio-emotional early childhood, namely girls who read and write more, boys follow their conscience less, boys are more physically aggressive, girls are more able to influence gossip, girls are more self-controlling, boys more often get into trouble from externals, and girls pay more attention to teachers in class. There is a strategy to educate boys, namely to invite boys to be more sensitive, reduce physical aggressiveness, and increase achievement in school. As for the strategy in educating girls, namely appreciating and supporting girls in their sensitivity and nurture, and encourage girls to be more active in expressing their aspirations

Keywords: Dysmilarity, gender, cognitive, social-emotional, child.
Incorporating Pacific Ocean climate information to enhance the tree-ring-based streamflow reconstruction skill
Authors: Bukhary Saria , Kalra Ajay , Ahmad Sajjad 
Published: December 2020

Abstract
The Sacramento River Basin (SRB) and the San Joaquin River Basin (JRB) have a history of recurring droughts. Both are important for California, being the crucial source of water supply. The available instrumental records may not depict the long-term hydrologic variability encompassing the duration and frequency of the historic low flow events. Thus, streamflow reconstruction becomes important in the current scenario of climatic alteration, escalating population and growing water needs. Studies have shown that Pacific Decadal Oscillation (PDO), Southern Oscillation Index (SOI), and Pacific Ocean sea surface temperature (SST) influence the precipitation and streamflow volumes of southwestern United States, particularly California. The focus of this study is to enhance the traditional tree-ring chronology (TRC)-based streamflow reconstruction approach by incorporating the predictors of SST, PDO, and SOI together with TRC, in a stepwise linear regression (SLR) model. The methodology was successfully applied to selected gauges located in the SRB and the JRB using five SLR models (SLR 1–5), and reconstructions were developed from 1801 to 1980 with an overlap period of 1933–1980. An improved reconstruction skill was demonstrated by using SST in combination with TRC (SLR-3 and SLR-5) (calibration r2 = 0.6–0.91 and cross-validation r² = 0.44–0.74) compared with using TRC only (SLR-1), or TRC along with SOI and PDO (SLR-2; calibration r2 = 0.51–0.78 and cross-validation r² = 0.41–0.68).
RECENTES ALTERAÇÕES NAS GELEIRAS E NOS SISTEMAS PARAGLACIAIS, ANTÁRTICA MARÍTIMA
Authors: Perondi Cleiva , Rosa Kátia Kellem da , Petsch Carina , Idalino Filipe Daros , Oliveira Manoela Araújo Gonçalves de , Lorenz Júlia Lopes , Vieira Rosemary , Simões Jefferson Cardia 
Published: December 2020

O artigo investiga alterações nos sistemas paraglaciais e interconexões com a retração glacial em setores da ilha Rei George, Antártica Marítima. Foi analisado o ambiente proglacial, resultado da deglaciação recente, e identificados os tipos de formas de relevo em suas diferentes escalas. Estes registros são úteis para a reconstrução de estágios sucessivos evolutivos no sistema paraglacial. Os resultados demonstraram que o ambiente marginal às geleiras e sistemas paraglaciais estão evoluindo na ilha Rei George e há novas paisagens. Algumas geleiras apresentaram uma mudança importante entre 2000 e 2019, onde sua classificação mudou de geleira de terminação marinha para geleira de terminação terrestre (não-marinha). Atualmente há 21 geleiras com término em terra na ilha Rei George (correspondendo a 31% das geleiras) e 11 destas estão localizadas na Baía do Almirantado. 25% destas geleiras eram marinhas em 2000. Os novos ambientes paraglaciais (desde 2000) têm 1,7 km2 ao total de área. Os ambientes marginais às geleiras (como as geleiras Ecology, Wanda, Windy, Anna Sul e Baranowski) mostraram formas de gênese não glacial, como planície de lavagem, depositos de tálus, ravinas e canais fluviais no sistema paraglacial recente. O mapeamento geomorfológico evidencia que os processos paraglaciais se diferenciam entre os ambientes marginais ao gelo das geleiras, não são padronizados.
Palavras-chave: processos geomorfológicos glaciais, áreas livres de gelo, mudanças climáticas.
Synthesis, Cytotoxicity Evaluation and Molecular Docking of Fluorine
Containing Hexahydroquinoline-3-Carbonitrile Derivatives
Authors: Teraiya Nishith , Karki Subhas S. , Chauhan Ashlesha 
Published: December 2020

Background:
Fluorine containing Hexahydroquinoline-3-carbonitrile derivatives were found to have potent cytotoxicity. Further, Fluorine can modulate pharmacokinetic and pharmacodynamic profile of drugs. Hence, new derivatives
containing fluorine were explored as potential cytotoxic agents.

Objective:
Difluoro substituted compounds containing aromatic/heteroaromatic rings were designed, synthesized and
screened for in vitro cytotoxicity on cancer cell lines. The active compounds were subjected to docking on Mcl-1 and
ADME/T prediction.

Methods:
The synthesized compounds were characterized using various spectral techniques like FT-IR, 1
H NMR, 13C NMR
and Mass spectra. Compounds were screened for cytotoxicity on NCI-60 cell lines at National Cancer Institute. The active
compounds were evaluated additionally by MTT and SRB assay.

Results:
Compounds (6l and 6o) showed maximum cytotoxicity with (% GI) of 69 and 63.7 at 10 µM drug concentration
respectively. Compound 6i showed potent cytotoxicity with GI50 of 7.2 µM against Ishikawa cell line. Compound 6o was
nearly as active as reference with IC50 of 9.39 µM and 13.54 µM against HT-29 and HCT-116 respectively and compound 6l
also showed equal potency to that of reference with IC50 of 9.66 µM against Caco-2. Compounds 6i, 6o and 6l showed high
docking score suggesting their cytotoxicity. Further, ADME/T prediction revealed that all the compounds have drug likeness
properties.

Conclusion:
Enhanced lipophilic interaction of compounds due to presence of fluorine in compounds 6i and 6l was revealed
during docking study. Compound 6i can be explored as a lead molecule against other endometrial cancer in futuristic drug
development.
Expeditious Preparation of N-Fmoc 2-Aminoethanesulfonyl Chlorides with Functionalized 1-Substituents
Authors: Haiouani Kheira , Chen Xingpeng , Xu Jiaxi 
Published: December 2020

Aim and Objective: [(9H-Fluoren-9-yl)methoxy]carbonyl (Fmoc)-protected 2-aminoethanesulfonyl
chlorides with various functionalized 1-substituents may be of use as building blocks for the Fmoc strategic
synthesis of sulfonopeptides.

Materials and Method:
Fmoc-protected 2-aminoethanesulfonyl chlorides with different functionalized 1-
substituents were synthesized via radical addition of N-Fmoc allylamine and xanthates with functionalized Ssubstituents,
and subsequent oxidative chlorination with N-chlorosuccimide/HCl.

Results:
Fmoc-protected 2-aminoethanesulfonyl chlorides with different functionalized 1-substituents were
synthesized via radical addition and subsequent oxidative chlorination. The mechanism on the oxidative chlorination
is proposed.

Conclusion:
The current method is an expeditious, convenient, and practical method for the synthesis of Fmocprotected
2-aminoethanesulfonyl chlorides with different functionalized 1-substituents in good to excellent
yields.
Pharmacological Management of Diabetes for Reducing Glucose Levels and Cardiovascular Disease Risk: What Evidence in South Asians?
Authors: Ghouri Nazim , Javed Hareem , Sattar Naveed 
Published: December 2020

Introduction:
South Asians experience more type 2 diabetes, which is earlier in onset
and with more rapid glycaemic deterioration, although average body mass indices are lower than in
whites. Cardiovascular outcomes from diabetes drug trials are now reported as standard, with data
from newer therapies influencing patient management. However, less is known of the effect of
such therapies in South Asians. The aim of this narrative review was to extract, wherever possible,
the glucose-lowering efficacy and cardiovascular and renal outcome data for these therapies in
South Asians.

Discussion:
Despite the higher prevalence and global burden of type 2 diabetes and adverse outcomes
in South Asians, they remain underrepresented in global trials. Even when recruited, the current
method of classifying ethnicity does not commonly allow South Asian data to be extracted and
reported separately from all Asians. Interrogation of available trial data suggests broadly comparable
effects on glycaemia and weight in Asians to other ethnicities with use of glucagon-like peptide
1 receptor agonists (GLP-1 RAs), but a potentially early, albeit marginally, greater glycaemia benefit
with Dipeptidyl peptidase-4 inhibitors (DPP4is) which may not be sustained. Furthermore, there
appears a potentially greater glycaemia benefit with use of sodium-glucose transport protein 2 inhibitors
(SGLT2is) in Asians compared to whites. Whether such findings are true in all Asians subgroups
requires further direct study. For cardiovascular outcomes, available data suggest at least
comparable and potentially greater outcome benefits in Asians; point estimates were more
favourable for Asians in the vast majority of GLP-1 RAs and SGLT2is outcome trials. It was, however,
impossible to determine whether the effects were similar across all Asian subgroups.

Conclusion:
We conclude that trialists should be encouraged to record ethnicity with better granularity
to allow differing ethnic groups data to be better interrogated. In the meantime, doctors
should, where possible, confidently follow newer guidelines for the use of newer glucose lowering
agents for treating glycaemia and for the prevention of cardiovascular and cardiorenal complications
in South Asian people with type 2 diabetes.
Sentinel Lymph Node Mapping in Early Stage Endometrial Cancer Patients in Low-Resource Settings
Authors: Negm Mahmoud Abubakr , Refaie Ashraf Nasr , El-Sayed Magdi Ragab , Abdel Azeez Hesham R. , Gad Abdalla Hassan , Wasfy Mohamed Ahmed , Nasr Ahmed Abobakr 
Published: December 2020

Background:
Lymph node status has a prognostic role in endometrial cancer patients and
it determines the adjuvant therapy to be administered postoperatively. Complete lymphadenectomy
carries the risk of serious complications. Sentinel Lymph Node mapping (SLN) may be a good option
to reduce these complications. Cervical injection of methylene blue may be a suitable technique
for SLN mapping, which can be applied in low-resource institutes.

Objective:
Assessment of the detection rate, sensitivity and false negative rate of SLN mapping
using cervical injection of methylene blue.

Methods:
A total of 76 patients with early stage endometrial cancer were included. Methylene blue
was injected into the cervix prior to surgery and open surgical approach was used. SLNs were detected
by direct visualization of stained lymph nodes after opening of pelvic spaces. SLNs were
excised and pelvic lymphadenectomy was performed. Para-aortic lymphadenectomy was performed
when indicated and surgically feasible. Specimens were examined by H&E staining.

Results:
The SLN detection rate was 47.4% and all detected SLNs were pelvic nodes. All patients
with lymph node metastasis had metastatic disease in their SLNs. The technique had 100% sensitivity,
100% negative predictive value and a 0% false-negative rate. An inverse relationship between
SLN detection and BMI was detected.

Conclusion:
Cervical injection of methylene blue dye with an open approach to detect SLNs in
patients with early stage endometrial cancer has a low detection rate. The most important factor
associated with failed mapping is increased BMI. Further trials are needed to investigate the usefulness
of this technique.