bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spectrin-based Membrane Mechanics Is Asymmetric and Remodels during Neural Development
Authors: Jia Ru , Chai Yongping , Xie Chao , Liu Gai , Zhu Zhiwen , Huang Kaiyao , Li Wei , Ou Guangshuo 
Published: April 2020

AbstractPerturbation of spectrin-based membrane mechanics causes hereditary elliptocytosis and spinocerebellar ataxia, but the underlying cellular basis of pathogenesis remains unclear. Here, we introduced the conserved disease-associated spectrin mutations into the C. elegans genome and studied the contribution of spectrin to neuronal migration and dendrite formation in developing larvae. The loss of spectrin generates an ectopic actin polymerization outside of the existing front and secondary membrane protrusions, leading to defective neuronal positioning and dendrite morphology in adult animals. Spectrin accumulates in the lateral and the rear of migrating neuroblasts and redistributes from the soma into the newly formed dendrites, indicating that the spectrin-based membrane skeleton is asymmetric and remodels to regulate actin assembly and cell shape during development. We affinity-purified spectrin from C. elegans and showed that its binding partner ankyrin functions with spectrin. Asymmetry and remodeling of membrane skeleton may enable spatiotemporal modulation of membrane mechanics for distinct developmental events.Significance StatementThe biomechanical regulation of neural development is largely unknown. The spectrin-based membrane skeleton is essential for the structural integrity of the plasma membrane. This study addresses the function and behavior of spectrin in neuroblast migration and dendrite formation. The loss of spectrin generates an ectopic actin polymerization outside of the existing front, leading to defective neuronal positioning and dendrite morphology. Spectrin is absent from the leading edge but accumulates in the posterior of migrating neuroblasts and redistributes from the soma into the nascent dendrites, indicating that the membrane skeleton is asymmetric and remodels. Asymmetry and remodeling of the membrane skeleton may enable spatiotemporal modulation of membrane mechanics for distinct developmental events.
Predicting Antimicrobial Resistance Using Conserved Genes
Authors: Nguyen Marcus , Olson Robert , Shukla Maulik , VanOeffelen Margo , Davis James J. 
Published: April 2020

AbstractA growing number of studies have shown that machine learning algorithms can be used to accurately predict antimicrobial resistance (AMR) phenotypes from bacterial sequence data. In these studies, models are typically trained using input features derived from comprehensive sets of known AMR genes or whole genome sequences. However, it can be difficult to determine whether genomes and their corresponding sets of AMR genes are complete when sequencing contaminated or metagenomic samples. In this study, we explore the possibility of using incomplete genome sequence data to predict AMR phenotypes. Machine learning models were built from randomly-selected sets of core genes that are held in common among the members of a species, and the AMR-conferring genes were removed based on their protein annotations. ForKlebsiella pneumoniae,Mycobacterium tuberculosis,Salmonella enterica, andStaphylococcus aureus, we report that it is possible to classify susceptible and resistant phenotypes with average F1 scores ranging from 0.80-0.89 with as few as 100 conserved non-AMR genes, with very major error rates ranging from 0.11-0.23 and major error rates ranging from 0.10-0.20. Models built from core genes have predictive power in the cases where the primary AMR mechanism results from SNPs or horizontal gene transfer. By randomly sampling non-overlapping sets of core genes for use in these models, we show that F1 scores and error rates are stable and have little variance between replicates. Potential biases from strain-specific SNPs, phylogenetic sampling, and imbalances in the phylogenetic distribution of susceptible and resistant strains do not appear to have an impact on this result. Although these small core gene models have lower accuracies and higher error rates than models built from the corresponding assembled genomes, the results suggest that sufficient variation exists in the core non-AMR genes of a species for predicting AMR phenotypes. Overall this study suggests that building models from conserved genes may be a potentially useful strategy for predicting AMR phenotypes when genomes are incomplete.
Gut expressed vitellogenin is hijacked by a virus to facilitate its spread
Authors: He Ya-Zhou , Wang Yu-Meng , Yin Tian-Yan , Liu Shu-Sheng , Wang Xiao-Wei 
Published: April 2020

AbstractInsect vitellogenin (Vg) has been considered to be synthesized in the female fat body, secreted into hemolymph, and taken up by developing oocytes to serve as nutrition for embryo development. However, the expression and function of Vg in other tissues are largely unknown. Here, we show that Vg is expressed in the whitefly midgut epithelial cells and secreted into gut lumen where it binds to the microvillar membrane. Furthermore, we find that the midgut expressed Vg is hijacked byTomato yellow leaf curl virusto facilitate the movement of virus crossing the midgut wall. Silencing of Vg or immune-blocking the interaction between viral coat protein and midgut Vg inhibited virus movement across the midgut wall and decreased virus transmission. Our findings show possession of functional Vg in the midgut of an insect, and reveal a novel mechanism involved in the movement of virus crossing the midgut barrier of vector insect.
Novel structural components generate distinct type VI secretion system anchoring modes
Authors: Bernal Patricia , Furniss R. Christopher D. , Fecht Selina , Leung Rhoda C.Y. , Spiga Livia , Mavridou Despoina A.I. , Filloux Alain 
Published: April 2020

ABSTRACTThe type VI secretion system (T6SS) is a phage-derived contractile nanomachine primarily involved in interbacterial competition. Its pivotal component, TssA, is indispensable for the assembly of the T6SS sheath structure, the contraction of which propels a payload of effector proteins into neighboring cells. Despite their key function, TssA proteins exhibit unexpected diversity and exist in two major forms, a short (TssAS) and a long (TssAL) TssA. Whilst TssALproteins interact with a partner, called TagA, to anchor the distal end of the extended sheath, the mechanism for the stabilization of TssAS-containing T6SSs remains unknown. Here we discover a novel class of structural components that interact with short TssA proteins and contribute to T6SS assembly by stabilizing the polymerizing sheath from the baseplate. We demonstrate that the presence of these components is important for full sheath extension and optimal firing. Moreover, we show that the pairing of each form of TssA with a different class of sheath stabilization proteins results in T6SS apparatuses that either reside in the cell for a while or fire immediately after sheath extension, thus giving rise to different aggression behaviors. We propose that this functional diversity could contribute to the specialization of the T6SS to suit bacterial lifestyles in diverse environmental niches.
Organisation and management of grey literature in select social science libraries
Authors: Bhatt R.K. , Singh Monika , Rana Jyoti , Kumar Jatin 
Published: April 2020

Purpose
This paper aims to study the organisation and management of grey literature (GL) to select social science libraries. Further, the study is an attempt to make known the types of GL; extent of demand and the frequency of use of GL; GL collection in other languages; workshops or training programmes or seminars for GL; bibliographic control for GL, etc., in select social science libraries.

Design/methodology/approach
A survey with the help of a questionnaire followed by interviews was conducted among the social science libraries understudy to explore the issues and challenges involved in organizing and managing GL.

Findings
The study finds that 83.3% of libraries have a separate wing for the management of GL and the majority of the GL acquired by the library are accessed through compact disc and are present in digital form access via online public access catalog.

Originality/value
The paper explores the organisation and management of GL by social science libraries, which is a unique study on its own as the management of GL is not much popular here in India. The study paves the path for more issues to be explored in the area i.e. GL.
Influence of the chamber inclination angle and heat-generating element location on thermal convection of power-law medium in a chamber
Authors: Loenko Darya , Shenoy Aroon , Sheremet Mikhail 
Published: April 2020

PurposeThis paper aims to study the mathematical modeling of passive cooling systems for electronic devices. Improving heat transfer is facilitated by the correct choice of the working fluid and the geometric configuration of the engineering cavity; therefore, this work is devoted to the analysis of the influence of the position of the heat-generating element and the tilted angle of the electronic cabinet on the thermal convection of a non-Newtonian fluid.Design/methodology/approachThe area of interest is a square cavity with two cold vertical walls, while the horizontal boundaries are adiabatic. An element of constant volumetric heat generation is placed on the lower wall of the chamber. The problem is described by Navier–Stokes partial differential equations using dimensionless stream function and vorticity. The numerical solution is based on the developed computational code using the finite difference technique and a uniform rectangular grid.FindingsThe key conclusions of this work are the results of a detailed analysis of streamlines and isotherms, the average Nusselt number and profiles of the average heater temperature. It was found that more intensive cooling of the heat-generating element occurs when the cavity is filled with a pseudoplastic fluid (n < 1) and not inclined (α = 0). The Rayleigh number of Ra = 105and the thermal conductivity ratio ofk = 100 are characterized by the most positive effect.Originality/valueThe originality of the research lies in both the study of thermal convection in a square chamber filled with power-law fluid under the influence of a volumetric heat production element and the analysis of the influence of geometric and thermophysical parameters characterizing the considered process.
Magneto-hydrodynamics of a solid-liquid two-phase fluid in rotating channel due to peristaltic wavy movement
Authors: Haider Sajjad , Ijaz Nouman , Zeeshan A. , Li Yun-Zhang 
Published: April 2020

Purpose
Numerous researchers have probed the peristaltic flows because of their immense usage in industrial engineering, biomedical engineering and biological sciences. However, the investigation of peristaltic flow in two-phase fluid of a rotating frame in the presence of a magnetic field has not been yet discussed. Therefore, to fulfill this gap in the existing literature, this paper will explicate the peristaltic flow of two-phase fluid across a rotating channel with the effect of wall properties in the presence of a magnetic field. The purpose of this study is to investigate the two-phase velocity distribution and rotation parameter when magneto-hydrodynamics is applied.

Design/methodology/approach
The constituent equations are solved under the condition of low Reynolds number and long wavelength. The exact method is used to attain the subsequent equations and a comprehensive graphical study for fluid phase, particulate phase velocity and flow rates are furnished. The impacts of pertinent parameters, magnetic field and rotation are discussed in detail.

Findings
It is witnessed that the velocity profile of particulate phase gets higher values for the same parameters as compared to the fluid phase velocity. Moreover, the axial velocity increases with different values of particle volume fraction, but in case of magnetic field and rotation parameter, it shows the opposite behavior.

Practical implications
The outcomes of study have viable industrial implementations in systems comprising solid-liquid based flows of fluids involving peristaltic movement.

Originality/value
The investigation of peristaltic flow in two-phase fluid of a rotating frame in the presence of a magnetic field has not been yet discussed. Therefore, to fulfill this gap, the present study will explicate the peristaltic flow of two-phase fluid across a rotating channel with the effect of wall properties in the presence of magnetic field.
Are Islamic mutual funds exposed to lower risk compared to their conventional counterparts?
Authors: Naveed Farrukh , Khawaja Idrees , Maroof Lubna 
Published: April 2020

Purpose
This study aims to comparatively analyze the systematic, idiosyncratic and downside risk exposure of both Islamic and conventional funds in Pakistan to see which of the funds has higher risk exposure.

Design/methodology/approach
The study analyzes different types of risks involved in both Islamic and conventional funds for the period from 2009 to 2016 by using different risk measures. For systematic and idiosyncratic risk single factor CAPM and multifactor models such as Fama French three factors model and Carhart four factors model are used. For downside risk analysis different measures such as downside beta, relative beta, value at risk and expected short fall are used.

Findings
The study finds that Islamic funds have lower risk exposure (including total, systematic, idiosyncratic and downside risk) compared with their conventional counterparts in most of the sample years, and hence, making them appear more attractive for investment especially for Sharīʿah-compliant investors preferring low risk preferences.

Practical implications
As this study shows, Islamic mutual funds exhibit lower risk exposure than their conventional counterparts so investors with lower risk preferences can invest in these kinds of funds. In this way, this research provides the input to the individual investors (especially Sharīʿah-compliant investors who want to avoid interest based investment) to help them with their investment decisions as they can make a more diversified portfolio by considering Islamic funds as a mean for reducing the risk exposure.

Originality/value
To the best of the author’s knowledge, this study is the first attempt at world level in looking at the comparative risk analysis of various types of the risks as follows: systematic, idiosyncratic and downside risk, for both Islamic and conventional funds, and thus, provides significant contribution in the literature of mutual funds.
Audit quality, political connections and information asymmetry: evidence from banks in gulf co-operation council countries
Authors: Tessema Abiot 
Published: April 2020

PurposeThe purpose of this paper is to investigate the impact of audit quality on information asymmetry for a sample of leading listed local banks in the Gulf Cooperation Council (GCC). In addition, the paper examines whether a firm's political connections moderate the association between audit quality and information asymmetry.Design/methodology/approachThe author employs country fixed effects to examine the impact of audit quality on information asymmetry. The paper uses a sample of 49 leading listed local banks across the GCC and 236 bank-year observations, over the period of 2012–2016.FindingsUsing trading volume, trade value and stock return volatility as proxies for information asymmetry and audit quality through auditors' opinion and audit size, the paper documents that audit quality plays an important role in improving the quality of financial information reporting by providing greater independent assurance of the credibility of financial reports. The paper also documents that a firm's political connections have no effect on the association between audit quality and information asymmetry, indicating that the beneficial effects of audit quality are no greater for politically connected firms than for similar but politically unconnected firms.Practical implicationsThe findings of the study help policymakers, standard-setters and regulators to understand the potential adverse effect of political connections on the role of audit quality on information asymmetry. The study also provides important insights for audit regulators to better identify and understand the benefits of audit quality and to take policy matters that influence audit quality seriously.Originality/valueThe study increases our understanding of the impact of audit quality on the level of information asymmetry in different economic, legal and political institutions, regulatory and litigation incentives and social contexts compared to that of research conducted using data collected from developed and other emerging countries. This will help to widen our knowledge on the role of audit quality on information asymmetry across the globe.
The effects of materialism on consumer evaluation of sustainable synthetic (lab-grown) products
Authors: Keech Jessica , Morrin Maureen , Podoshen Jeffrey Steven 
Published: April 2020

Purpose
The increasing desire of consumers for socially responsible luxury products combined with fluctuating supplies in consumer markets are leading various industries to seek alternative sources to be able to meet the needs of its customers. One possible solution that may meet the demands of the future is lab-grown products. Because these products confer multiple benefits, this study aims to investigate the most effective ways to appeal to consumers by aligning the benefits of the products with their values as marketers seek to find effective promotion for these items.

Design/methodology/approach
We examine the effectiveness of an ethical positioning strategy for two types of luxury lab-grown (synthetic) products among high versus low materialism consumers in three experiments.

Findings
Findings suggest that a positioning strategy stressing product ethicality is more effective for low materialism consumers, whereas the strategy is less effective, and may even backfire, for high materialism consumers. The impact on social status consumers perceive from a lab-grown product explains why this effect occurs among low materialism consumers. Therefore, marketers should take caution and use specific appeals for different segments based on values such as consumers’ materialism levels.

Originality/value
If lab-grown products represent the wave of the future, it is important to understand how consumers will respond to this emerging technology and how promotion strategies may enhance their evaluation.
The media as an investigative resource: reflections from English cold-case units
Authors: Bennett Kirsty 
Published: April 2020

Purpose
The use of the media in live cases has been explored in terms of its use and value to an investigation. However, it is unclear as to whether engaging with the media in cold-case investigations results in a positive or negative reception, and what impact this can have on a case’s possibility for progression. Because of the passage of time and a lack of, or a failed, prosecution means that the approach to media use needs to be different. The purpose of this paper is to explore how the media could be used as an investigative resource for cold cases.

Design/methodology/approach
This study is a result of a 7-month observation period with a 2-force collaborative cold-case team in England, and supplemented with interviews with 12 experienced cold-case detectives. Using inductive thematic analysis, the themes identified allow an exploration of detectives’ use of the media and the effect that this has on progressing cases. Further, there is discussion as to whether the media’s involvement is positive or negative.

Findings
The overarching theme is that when using the media, cold-case detectives are met with a positive reception and interest. The media can be used to obtain information, particularly in cases with minimal information, and it is important to use murder-anniversaries to obtain help from the public. However, this needs to be a carefully managed strategy as the media coverage can be negative, including inaccurate or inappropriate reporting which can be of detriment to the investigation.

Originality/value
To the best of the author’s knowledge, this is the first paper to explore how cold-case detectives have used the media to progress cases, and the findings demonstrate that when the public are encouraged to come forward with information, there is a better chance of case progression. Further research is required to explore how all cold cases can receive appropriate coverage.
A decision model for selecting a safety data sheet management system using fuzzy TOPSIS
Authors: Ahmed Abdulaziz , Naji Ahmed , Tseng Ming-Lang 
Published: April 2020

Purpose
Safety data sheets are documents developed by chemical manufacturers to identify and label hazardous materials. The occupational safety and health administration regulations state that employers must make safety data sheets available for employees. When firms use hundreds of chemicals, tracking their safety data sheets becomes difficult. Safety Data Sheet Management Systems are developed to track safety data sheets. This paper aims to propose a multi-attribute decision-making framework for selecting a Safety Data Sheet Management System.

Design/methodology/approach
A total of 12 attributes are proposed based on a real-life project conducted at a firm in New York and the software selection models existed in the literature. Fuzzy technique for order of preference by similarity to ideal solution is used to assess the proposed attributes and alternatives. A case study and sensitivity analysis are conducted to show the robustness of the proposed model. Fuzzy analytical hierarchy process is used for validation.

Findings
Safety Data Sheet Management System is important for firms to track and manage safety data sheets. The proposed framework is practical and easy to implement.

Practical implications
The proposed decision model is useful for firms to select a proper Safety Data Sheet Management System. The system developers can use the model to update their systems.

Originality/value
This paper develops a new multi-attribute decision-making model for selecting a Safety Data Sheet Management System. To the best of the authors’ knowledge, no previous study has developed such a model.