bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimated internal exposure doses due to indoor radiocaesium contamination in residential houses after the Fukushima nuclear accident
Authors: Yoshida-Ohuchi Hiroko , Shinohara Naohide 
Published: October 2020

AbstractThis work first reports the estimation of the internal exposure from ingestion of house dust and inhalation of aerosol, by employing a measured data on 137Cs activities, bioaccessibility (solubility to water and 1 M HCl), and particle size distribution. The house dust and aerosol samples were collected during the actual indoor cleaning by vacuuming and dusting, from 65 houses and buildings in proximity to the Fukushima Daiichi nuclear power plant (FDNPP) (1.6–16.1 km from the FDNPP) during a period from April 2016 to January 2019. Committed effective doses for an adult owing to the ingestion of house dust of 20 mg per day, which adheres to one’s hands through the hand-to-mouth, and those owing to inhalation of aerosol during dusting for 1.5 h while wearing a mask, were calculated using DCAL software for each house or building, as 1.13 µSv and 4.55 µSv as maximum doses, respectively (as of March 2011). Both the committed effective doses, owing to ingestion and inhalation, were inversely correlated with the distance from the FDNPP, and positively correlated with the indoor surface contamination.
Synthesis and application of gas diffusion cathodes in an advanced type of undivided electrochemical cell
Authors: Zaidi S. Z. J. , Luan Y. , Harito C. , Utari L. , Yuliarto B. , Walsh F. C. 
Published: October 2020

AbstractThis paper reports the oxidation of Remazol black B dye by employing iron ions catalyst based gas diffusion cathodes, (GDCs). A GDC was synthesized by using a layer of carbon black and iron ions catalyst for oxygen reduction to hydrogen peroxide. The results demonstrated around 97% decolorization of Remazol black-B dye for 50 min by iron ions catalyst based GDC. The degradation study was performed under electrogenerated hydrogen peroxide at a constant voltage of − 0.6 V vs Hg/HgSO4 in which the rate of degradation was correlated with hydrogen peroxide production. Overall, the GDC’s found to be effective method to degrade the dyes via electro-Fenton.
Specific cyprinid HIF isoforms contribute to cellular mitochondrial regulation
Authors: Chen Jing , Guan Lihong , Zou Ming , He Shunping , Li Dapeng , Chi Wei 
Published: October 2020

AbstractHypoxia-inducible factor 1 (HIF-1) functions as a master regulator of the cellular response to hypoxic stress. Two HIF-1α paralogs, HIF-1αA and HIF-1αB, were generated in euteleosts by the specific, third round of genome duplication, but one paralog was later lost in most families with the exception of cyprinid fish. How these duplicates function in mitochondrial regulation and whether their preservation contributes to the hypoxia tolerance demonstrated by cyprinid fish in freshwater environments is not clear. Here we demonstrated the divergent function of these two zebrafish Hif-1a paralogs through cellular approaches. The results showed that Hif-1aa played a role in tricarboxylic acid cycle by increasing the expression of Citrate synthase and the activity of mitochondrial complex II, and it also enhanced mitochondrial membrane potential and ROS production by reducing free Ca2+ in the cytosol. Hif-1ab promoted intracellular ATP content by up-regulating the activity of mitochondrial complexes I, III and IV and the expression of related genes. Furthermore, both the two zebrafish Hif-1a paralogs promoted mitochondrial mass and the expression level of mtDNA, contributing to mitochondrial biogenesis. Our study reveals the divergent functions of Hif-1aa and Hif-1ab in cellular mitochondrial regulation.
Understanding deep learning in land use classification based on Sentinel-2 time series
Authors: Campos-Taberner Manuel , García-Haro Francisco Javier , Martínez Beatriz , Izquierdo-Verdiguier Emma , Atzberger Clement , Camps-Valls Gustau , Gilabert María Amparo 
Published: October 2020

AbstractThe use of deep learning (DL) approaches for the analysis of remote sensing (RS) data is rapidly increasing. DL techniques have provided excellent results in applications ranging from parameter estimation to image classification and anomaly detection. Although the vast majority of studies report precision indicators, there is a lack of studies dealing with the interpretability of the predictions. This shortcoming hampers a wider adoption of DL approaches by a wider users community, as model’s decisions are not accountable. In applications that involve the management of public budgets or policy compliance, a better interpretability of predictions is strictly required. This work aims to deepen the understanding of a recurrent neural network for land use classification based on Sentinel-2 time series in the context of the European Common Agricultural Policy (CAP). This permits to address the relevance of predictors in the classification process leading to an improved understanding of the behaviour of the network. The conducted analysis demonstrates that the red and near infrared Sentinel-2 bands convey the most useful information. With respect to the temporal information, the features derived from summer acquisitions were the most influential. These results contribute to the understanding of models used for decision making in the CAP to accomplish the European Green Deal (EGD) designed in order to counteract climate change, to protect biodiversity and ecosystems, and to ensure a fair economic return for farmers.
Therapeutic benefit of Muse cells in a mouse model of amyotrophic lateral sclerosis
Authors: Yamashita Toru , Kushida Yoshihiro , Wakao Shohei , Tadokoro Koh , Nomura Emi , Omote Yoshio , Takemoto Mami , Hishikawa Nozomi , Ohta Yasuyuki , Dezawa Mari , Abe Koji 
Published: October 2020

AbstractAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive motor neuron loss. Muse cells are endogenous reparative pluripotent-like stem cells distributed in various tissues. They can selectively home to damaged sites after intravenous injection by sensing sphingosine-1-phosphate produced by damaged cells, then exert pleiotropic effects, including tissue protection and spontaneous differentiation into tissue-constituent cells. In G93A-transgenic ALS mice, intravenous injection of 5.0 × 104cells revealed successful homing of human-Muse cells to the lumbar spinal cords, mainly at the pia-mater and underneath white matter, and exhibited glia-like morphology and GFAP expression. In contrast, such homing or differentiation were not recognized in human mesenchymal stem cells but were instead distributed mainly in the lung. Relative to the vehicle groups, the Muse group significantly improved scores in the rotarod, hanging-wire and muscle strength of lower limbs, recovered the number of motor neurons, and alleviated denervation and myofiber atrophy in lower limb muscles. These results suggest that Muse cells homed in a lesion site-dependent manner and protected the spinal cord against motor neuron death. Muse cells might also be a promising cell source for the treatment of ALS patients.
Infliximab concentrations in two non-switching cohorts of patients with inflammatory bowel disease: originator vs. biosimilar
Authors: Martínez-Feito Ana , Bravo-Gallego Luz Yadira , Hernández-Breijo Borja , Diez Jesús , García-Ramirez Laura , Jaquotot Marta , Plasencia-Rodríguez Chamaida , Nozal Pilar , Mezcua Araceli , Martín- Arranz María Dolores , Pascual-Salcedo Dora 
Published: October 2020

AbstractBiosimilars are replacing originator compounds due to their similar effectiveness, safety and pharmacokinetics. Our objective was to compare the differences in pharmacokinetics and clinical outcomes between the originator infliximab (Ifx) and the biosimilar CT-P13 in a patient cohort with inflammatory bowel disease (IBD). Our cohort study included 86 patients from a historical and a prospective cohort from the start of infliximab treatment to 22 weeks later. Serum infliximab, antidrug antibody levels and other serum biomarkers were measured at weeks 0, 2, 6, 14 and 22. Remission outcomes were evaluated at weeks 14 and 22. Drug levels were measured prospectively and analysed using MANOVA. Of the 86 patients, 44 (51%) and 42 (49%) were administered the originator and CT-P13, respectively. Originator trough levels were higher than the biosimilar trough levels (35 vs. 21, 20.1 vs. 11, 6.6 vs. 2.9 and 4.3 vs. 1.7 μg/mL at weeks 2, 6, 14 and 22, respectively). A post-hoc analysis demonstrated changes in mean serum drug levels over time (p < 0.001) and according to the drug employed (p = 0.001). At week 22, 13 (81%) patients administered the originator achieved clinical remission compared with 5 (19%) patients with the biosimilar (p = 0.02). None of the patients administered the originator withdrew from the treatment compared with 7 for the biosimilar. During the study, there were significant differences in serum infliximab levels between the originator and the CT-P13 in the patients with IBD. The clinical outcomes were influenced by the type of compound administered.
Cancer risks associated with the germline MITF(E318K) variant
Authors: Guhan Samantha M. , Artomov Mykyta , McCormick Shelley , Njauw Ching -Ni , Stratigos Alexander J. , Shannon Kristen , Ellisen Leif W. , Tsao Hensin 
Published: October 2020

AbstractThe MITF(E318K) variant confers moderate risk for cutaneous melanoma. While there are small studies suggesting that this risk is associated with other malignancies (e.g. renal cell carcinoma), little is known about the role of this variant in specifying risk for other cancers. In this study, we perform a systematic review and meta-analysis of the published data as a backdrop to a whole-exome sequence(WES)-based characterization of MITF(E318K) risk for various cancers in sporadic samples from the TCGA and several genetically-enriched patient cohorts. We found minimal evidence of MITF(E318K)’s contribution to non-melanoma cancer risk among individuals with low inherited risks of melanoma (OR 1.168; 95% CI 0.78–1.74; p = 0.454), suggesting that earlier reports of an association between this variant and other malignancies may be related to shared environmental or polygenic risk factors rather than MITF(E318K). Interestingly, an association was observed with uterine carcinosarcoma, (OR 9.24; 95% CI 2.08–37.17; p = 0.024), which was not previously described. While more research needs to be completed, this study will help update cancer screening recommendations for patients with the MITF(E318K) variant.
Hyperthyroidism and vascular cell adhesion molecule-1 are associated with a low ankle-brachial index
Authors: Li Yu-Hsuan , Lee I-Te 
Published: October 2020

AbstractWe aimed to assess the ankle-brachial index (ABI) in patients with Graves’ disease. In the cross-sectional assessments, 81 patients with drug-naïve Graves’ disease and 235 with euthyroidism were enrolled. ABI and vascular cell adhesion molecule-1 (VCAM-1) levels were assessed. In the prospective follow-up, 32 patients with Graves’ disease were assessed again after antithyroid drugs for at least 4 weeks, and 32 age- and sex-matched controls with euthyroidism were also followed up. Patients with Graves’ disease had a higher VCAM-1 level (1309 ± 292 vs. 1009 ± 168 ng/mL, P < 0.001) and a lower ABI (0.98 ± 0.11 vs. 1.06 ± 0.10, P < 0.001) than those with euthyroidism. ABI was significantly lower in patients with hyperthyroidism and a high VCAM-1 level than in those with euthyroidism and a low VCAM-1 level (regression coefficient: − 0.050, 95% confidence interval [CI] between − 0.080 and − 0.019; P = 0.001). After treatment with antithyroid drugs, the change in free thyroxine (T4) level was inversely associated with the percentage change in ABI (regression coefficient: − 0.003, 95% CI between − 0.005 and − 0.001, P = 0.001). A synergistic effect of VCAM-1 and free T4 on ABI reduction was observed. After a longitudinal follow-up, an increase in ABI was significantly correlated with a decrease in the free T4 level.
Synthetic anticoagulant heparan sulfate attenuates liver ischemia reperfusion injury
Authors: Arnold Katelyn , Xu Yongmei , Liao Yi-En , Cooley Brian C. , Pawlinski Rafal , Liu Jian 
Published: October 2020

AbstractHeparan sulfate (HS) is a sulfated glycosaminoglycan abundant on the cell surface and in the extracellular matrix and has several biological activities including anticoagulation and anti-inflammation. Liver ischemia reperfusion injury is associated with coagulation and inflammatory responses. Here, we synthesized HS oligosaccharides with defined sulfation patterns and show that synthetic anticoagulant HS oligosaccharides limit liver ischemia reperfusion injury in a mouse model. Using a small targeted HS library, we demonstrate that an oligosaccharide that possesses both anticoagulant activity and binding affinity to HMGB1, the inflammatory target, decreases injury greater than oligosaccharides that only bind to HMGB1 or only have anticoagulant activity. HS oligosaccharides may represent a potential new therapeutic option for decreasing liver damage resulting from ischemia reperfusion injury.
Individual and group level personality change across the lifespan in dogs
Authors: Turcsán Borbála , Wallis Lisa , Berczik Judit , Range Friederike , Kubinyi Enikő , Virányi Zsófia 
Published: October 2020

AbstractIn humans, age-related changes in personality occur in a non-random fashion with respect to their direction, timing, and magnitude. In dogs, there are still gaps in our knowledge about the detailed dynamics of age-related personality changes. We analysed the personality of 217 Border collies aged from 0.5 to 15 years both cross-sectionally and longitudinally using a test battery, to specify age periods when changes most prominently occur, assess the magnitude of changes, and analyse individual differences in personality change. We found that similar to humans, changes in personality occur unevenly during the dogs’ life course, however, their dynamics seems to be specific for each trait. Activity-independence decreased mostly from puppyhood (0.5–1 years) to adolescence (> 1–2 years), then continued to decrease in a slowing rate. Novelty seeking did not change markedly until middle age (> 3–6 years), then showed a steady linear decrease. Problem orientation increased strongly until middle age then showed no marked changes in later age periods. We also revealed individual differences in personality change over time, and showed that a few individuals with potential age-related impairments significantly affected the general age trajectory of some traits. These results raise caution against the over-generalisation of global age trends in dogs.
Comparison of three lymph node staging systems in evaluating the prognosis of patients with pT3 esophageal squamous cell carcinoma
Authors: Liu Di-tian , Wang Lin-shuo , Chen Yu-ping , Chen Shao-bin 
Published: October 2020

AbstractTo explore the prognostic value of three lymph node staging systems, including number of positive lymph nodes (pN), lymph node ratio (LNR), and log odds of positive lymph nodes (LODDS), in patients with pT3 stage esophageal squamous cell carcinoma (ESCC). Data from 1667 patients with pT3 stage ESCC who underwent surgical resection were reviewed. The log-rank test was used to assess the differences in overall survival (OS) between groups. Multivariate analysis was performed to identify independent prognostic factors. The receiver operating characteristic curve was used to assess the prognostic accuracy of the three staging methods. The median survival time for the entire group was 48.0 months, and the 1-, 3- and 5-year OS rates were 83.9%, 55.1% and 66.6%, respectively. All three lymph node staging systems were significantly correlated with OS in univariate and multivariate analyses. However, LNR and LODDS staging systems could more accurately predict survival than the pN staging system in patients with < 15 lymph nodes dissected, while LODDS have the best prognostic homogeneity. All three staging systems could be used for prognostic assessment in pT3 stage ESCC. But LODDS staging system might be superior to the others due to its prognostic homogeneity.
Textile sensors platform for the selective and simultaneous detection of chloride ion and pH in sweat
Authors: Possanzini Luca , Decataldo Francesco , Mariani Federica , Gualandi Isacco , Tessarolo Marta , Scavetta Erika , Fraboni Beatrice 
Published: October 2020

AbstractThe development of wearable sensors, in particular fully-textile ones, is one of the most interesting open challenges in bioelectronics. Several and significant steps forward have been taken in the last decade in order to achieve a compact, lightweight, cost-effective, and easy to wear platform for healthcare and sport activities real-time monitoring. We have developed a fully textile, multi-thread biosensing platform that can detect different bioanalytes simultaneously without interference, and, as an example, we propose it for testing chloride ions (Cl−) concentration and pH level. The textile sensors are simple threads, based on natural and synthetic fibers, coated with the conducting polymer poly(3,4-ethylenedioxythiophene):poly(styrene-sulfonate) (PEDOT:PSS) and properly functionalized with either a nano-composite material or a chemical sensitive dye to obtain Cl− and pH selective sensing functionality, respectively. The single-thread sensors show excellent sensitivity, reproducibility, selectivity, long term stability and the ability to work with small volumes of solution. The performance of the developed textile devices is demonstrated both in buffer solution and in artificial human perspiration to perform on-demand and point-of-care epidermal fluids analysis. The possibility to easily knit or sew the thread sensors into fabrics opens up a new vision for a textile wearable multi-sensing platform achievable in the near future.
Association between urinary polycyclic aromatic hydrocarbons and hypertension in the Korean population: data from the Second Korean National Environmental Health Survey (2012–2014)
Authors: Lee Tae-Woo , Kim Dae Hwan , Ryu Ji Young 
Published: October 2020

AbstractPolycyclic aromatic hydrocarbons (PAHs) are environmental and occupational pollutants derived from incomplete combustion of organic materials, including wood and fossil fuels. Epidemiological studies have evaluated the association between PAH exposure and hypertension or cardiovascular disease in the general population, but the evidence is limited. In this study, we evaluated the association between urinary PAH metabolites and hypertension in the Korean adult population. A total of 6478 adults who participated in the Second Korean National Environmental Health Survey (2012–2014) were included. The differences in urinary concentrations of four PAH metabolites, including 1-hydroxypyrene, 2-hydroxyfluorene, 1-hydroxyphenanthrene, and 2-naphthol, were compared according to hypertension status using a general linear model. Adjusted odds ratios (aORs) for hypertension were calculated according to the quartile groups of urinary PAH metabolites after adjusting for age, sex, body mass index (BMI), smoking, and alcohol consumption in multiple logistic regression analyses. The estimated mean concentrations of urinary 1-hydroxyphenanthrene were significantly higher in the hypertension group than in the non-hypertension group. In 1-hydroxyphenanthrene, the OR for hypertension was significantly higher in the third and fourth quartile groups than in the first quartile group (third: OR 1.707, 95% CI 1.203–2.423, fourth: OR 1.604, 95% CI 1.158–2.223). No significant associations were detected for the other metabolites. Our results suggest an association between exposure to PAHs and hypertension in a Korean adult population. Further studies are required to evaluate the effects of low-dose long-term exposure to PAHs on hypertension and cardiovascular disease.
Electricity use is associated with residents’ vital data and lifestyles: observational study using an IT health support system in Japan
Authors: Yasukawa Keiji , Ishihara Yukio , Hirayama Fumi , Nakanishi Megumi , Utsumi Hideo , Koyama Susumu 
Published: October 2020

AbstractMotivated by developments in information technology, recording personal parameters with health devices is effective in health promotion. Today’s indoor individual lifestyles often involve using electrical appliances. We developed a health support system combined with wireless electricity monitoring and investigated whether electricity use is associated with residents’ vital data and lifestyles. We recruited 116 participants in February 2013. Their vital and electricity use data were collected daily. They completed a self-administered questionnaire. Among participants living alone, electricity from 20 February to 11 March 2013 was negatively associated with high-density lipoprotein (HDL) (P = 0.008) and positively associated with low-density lipoprotein (LDL) (P = 0.007) and neutral fat (P = 0.020) levels. Among all participants, electricity use was negatively associated with vegetable intake (P = 0.044) and step count (P = 0.040). Temperature sensitivity in winter was negatively associated with the LDL/HDL ratio for both men and women. For men, temperature sensitivity in winter was negatively related with alcohol intake; for women, it was positively related to body fat percentage and abdominal circumference and negatively correlated to vegetable intake. Temperature sensitivity in summer was positively associated with vegetable intake for men and women. In conclusion, electricity use was related to vital data and lifestyles and influenced by temperature.
Selection of endogenous control genes for normalising gene expression data derived from formalin-fixed paraffin-embedded tumour tissue
Authors: Smith Tim A. D. , AbdelKarem Omneya A. , Irlam-Jones Joely J. , Lane Brian , Valentine Helen , Bibby Becky A. S. , Denley Helen , Choudhury Ananya , West Catharine M. L. 
Published: October 2020

AbstractQuantitative real time polymerase chain reaction (qPCR) data are normalised using endogenous control genes. We aimed to: (1) demonstrate a pathway to identify endogenous control genes for qPCR analysis of formalin-fixed paraffin-embedded (FFPE) tissue using bladder cancer as an exemplar; and (2) examine the influence of probe length and sample age on PCR amplification and co-expression of candidate genes on apparent expression stability. RNA was extracted from prospective and retrospective samples and subject to qPCR using TaqMan human endogenous control arrays or single tube assays. Gene stability ranking was assessed using coefficient of variation (CoV), GeNorm and NormFinder. Co-expressed genes were identified from The Cancer Genome Atlas (TCGA) using the on-line gene regression analysis tool GRACE. Cycle threshold (Ct) values were lower for prospective (19.49 ± 2.53) vs retrospective (23.8 ± 3.32) tissues (p < 0.001) and shortervslonger probes. Co-expressed genes ranked as the most stable genes in the TCGA cohort by GeNorm when analysed together but ranked lower when analysed individually omitting co-expressed genes indicating bias. Stability values were < 1.5 for the 20 candidate genes in the prospective cohort. As they consistently ranked in the top ten by CoV, GeNorm and Normfinder,UBC, RPLP0, HMBS, GUSB, and TBPare the most suitable endogenous control genes for bladder cancer qPCR.