bluuebunny/crossref_metadata_2025_split huggingface dataset:
Should we remain hopeful? The key 8 weeks: Spatiotemporal epidemic characteristics of COVID-19 in Sichuan Province and its comparative analysis with other provinces in China and global epidemic trends
Authors: Xu Xinyin , Zeng Jing , Liu Runyou , Liu Yang , Zhou Xiaobo , Zhou Lijun , Dong Ting , Cha Yuxin , Wang Zhuo , Deng Ying , Zhang Yu , Feng Liao , Pu Chen , Wu Xianping , Zhong Bo 
Published: July 2020

Abstract
Background: The COVID-19 spread worldwide quickly. Exploring the epidemiological characteristics could provide a basis for responding to imported cases abroad and to formulate prevention and control strategies in areas where COVID-19 is still spreading rapidly. Methods: The number of confirmed cases, daily growth, incidence and length of time from the first reported case to the end of the local cases (i.e., non-overseas imported cases) were compared by spatial (geographical) and temporal classification and visualization of the development and changes of the epidemic situation by layers through maps. Results: In the first wave, a total of 539 cases were reported in Sichuan, with an incidence rate of 0.6462/100,000. The closer to Hubei the population centres were, the more pronounced the epidemic was. The peak in Sichuan Province occurred in the second week. Eight weeks after the Wuhan lockdown, the health crisis had eased. The longest epidemic length at the city level in China (except Wuhan, Taiwan, and Hong Kong) was 53 days, with a median of 23 days. Spatial autocorrelation analysis of China showed positive spatial correlation (Moran's Index >0, p<0.05). Most countries outside China began to experience a rapid rise in infection rates 4 weeks after their first case. Some European countries experienced that rise earlier than the USA. The pandemic in Germany, Spain, Italy, and China took 28, 29, 34, and 18 days, respectively, to reach the peak of daily infections, after their daily increase of up to 20 cases. During this time, countries in the African region and Southeast Asian region were at an early stage of infections, those in the Eastern Mediterranean region and region of the Americas were in a rapid growth phase. Conclusions: After the closure of the outbreak city, appropriate isolation and control measures in the next 8 weeks were key to control the outbreak, which reduced the peak value and length of the outbreak. Some countries with improved epidemic situations need to develop a continuous "local strategy at entry checkpoints" to respond to a possible second local epidemic.
Peer navigation for individuals with serious mental illness leaving jail: a randomized trial study protocol
Authors: Hailemariam Maji , Weinstock Lauren M , Johnson Jennifer E. 
Published: July 2020

Abstract
Background: Serious mental illness (SMI) is a prevalent public health problem affecting 25% of individuals in jail. Re-entry to the community following incarceration is a vulnerable time for justice-involved individuals with SMI. SMI requires prompt and ongoing access to mental health and other healthcare services. Methods: The study will: 1) develop a Mentoring And Peer Support (MAPS) intervention for post-release mental health and other service connection among jailed individuals with SMI, and 2) pilot-test the MAPS intervention to determine the feasibility and acceptability of the MAPS intervention. The primary outcomes will be to evaluate the feasibility and acceptability of the proposed recruitment methods and research design, of the intervention training methods, of delivering the enhanced peer-navigator and control interventions. Study samples include (focus groups, open trial), and a randomized pilot trial in a sample of 40 individuals with SMI re-entering the community after jail release. Secondary outcomes will include post-release enrollment in mental health, medical care, and substance use services. We will also evaluate reduction in psychiatric symptoms, improvements in functioning, adherence to psychiatric medications, fewer substance using days, fewer hospitalizations and suicide attempts, nights unstably housed, and time until rearrest. Discussion: This pilot study will evaluate the feasibility and acceptability of a peer navigation intervention for individuals with serious mental illness leaving jails. The study will serve as a formative work for a larger randomized controlled trial assessing the effectiveness of peer navigator intervention for (include the primary outcome) in this population.
Nanosecond Pulsed Electric Fields Enhance Mesenchymal Stem Cells Differentiation via DNMT1 regulated OCT4/NANOG gene expression
Authors: Li Kejia , Ning Tong , Wang Hao , Jiang Yangzi , Zhang Jue , GE Zigang 
Published: July 2020

Abstract
Background: Multiple strategies have been proposed to promote the differentiation potential of mesenchymal stem cells (MSCs), which is the fundamental property in tissue formation and regeneration. However, these strategies are relatively inefficient that limit the application. In this study, we reported a novel and efficient strategy, nanosecond pulsed electric fields (nsPEFs) stimulation, which can enhance the trilineage differentiation potential of MSCs; and further explained the mechanism behind.Methods: We used histological staining to screen out the nsPEFs parameters that promoted the trilineage differentiation potential of MSCs, and further proved the effect of nsPEFs by detecting the functional genes. In order to explore the corresponding mechanism, we examined the expression of pluripotency genes and the methylation status of their promoters. Finally, we targeted the DNA methyltransferase which was affected by nsPEFs. Results: The trilineage differentiation of bone marrow derived MSCs was significantly enhanced in vitro by simply pre-treated with 5 pulses of nsPEFs stimulation (energy levels as 10 ns, 20 kV/cm; 100 ns, 10 kV/cm), due to that the nsPEFs demethylated the promoters of stem cell pluripotency genes OCT4 and NANOG through instantaneous downregulation of DNA methylation transferase 1 (DNMT1), thereby increased the expression of OCT4 and NANOG for up to 3 days, and created a treatment window period of stem cells.Conclusions: In summary, nsPEFs can enhance MSCs differentiation via the epigenetic regulation, and could be a safe and effective strategy for future stem cell application
Research on Position Optimization of Badminton Doubles Based on Wireless Sensor and Human-computer Interactive Training
Authors: Yao Bo , Liang Na 
Published: July 2020

Abstract
In the continuous development process, robot technology based on multimedia interactions has been widely used in aerospace, medical, education and service industries, and the relationship between robots and humans is getting closer. The improvement of robot intelligence is a process of continuously learning the outside world. Since the introduction of multimedia human-computer interaction technology in 1959, it has provided more and more technical support for human research robots. This paper studies the characteristics of badminton competition, the characteristics of technical and tactics and the collection requirements of on-site training, and completes the technical description of the badminton doubles station. Through the digitalization of the gait-based sensation, the automated acquisition of the gait technology, the simultaneously with the video, the high-speed imaging system, the system implement information integration. Subsequently, the statistical analysis of badminton multimedia techniques and tactics was designed. The skills and tactics of athletes were expressed in the form of video, graphics and text. The operators can carry out the technical and tactical analysis implementation process through good human-computer interaction, and increase the end user's game information. Attention and understanding strengthen the strength and depth of technical and tactical ability analysis. Finally, the station optimization plan for badminton doubles is proposed.
Artificial neural networks improve LDCT lung cancer screening: A comparative validation study
Authors: Hsu Yin-Chen , Tsai Yuan-Hsiung , Weng Hsu-Huei , Hsu Li-Sheng , Tsai Ying-Huang , Lin Yu-Ching , Hung Ming-Szu , Fang Yu-Hung , Chen Chien-Wei 
Published: July 2020

Abstract
Background: This study proposes a prediction model for the automatic assessment of lung cancer risk based on an artificial neural network (ANN) with a data-driven approach to the low-dose computed tomography (LDCT) standardized structure report.Methods: This comparative validation study analysed a prospective cohort from Chiayi Chang Gung Memorial Hospital, Taiwan. In total, 836 asymptomatic patients who had undergone LDCT scans between February 2017 and August 2018 were included, comprising 27 lung cancer cases and 809 controls. A derivation cohort of 602 participants (19 lung cancer cases and 583 controls) was collected to construct the ANN prediction model. A comparative validation of the ANN and Lung-RADS was conducted with a prospective cohort of 234 participants (8 lung cancer cases and 226 controls). The areas under the curves (AUCs) of the receiver operating characteristic (ROC) curves were used to compare the prediction models.Results: At the cut-off of category 3, the Lung-RADS had a sensitivity of 12.5%, specificity of 96.0%, positive predictive value of 10.0%, and negative predictive value of 96.9%. At its optimal cut-off value, the ANN had a sensitivity of 75.0%, specificity of 85.0%, positive predictive value of 15.0%, and negative predictive value of 99.0%. The area under the ROC curve was 0.764 for the Lung-RADS and 0.873 for the ANN (P=0.01). The heatmap plot demonstrates the leading items, i.e., solid nodules, partially solid nodules, and ground-glass nodules, as the significant predictors of malignant outcomes.Conclusions: Compared to the Lung-RADS, the ANN provided better sensitivity for the detection of lung cancer in an Asian population. In addition, the ANN provided a more refined discriminative ability than the Lung-RADS for lung cancer risk stratification with population-specific demographic characteristics. When lung nodules are detected and documented in a standardized structured report, ANNs may better provide important insights for lung cancer prediction than conventional rule-based criteria.Trial registrationNot applicable.
A novel strategy for efficient disaccharides synthesis from glucose by β-glucosidase
Authors: Niu Kangle , Liu Zhengyao , Feng Yuhui , Gao Tianlong , Wang Zhenzhen , Zhang Piaopiao , Du Zhiqiang , Gao Daming , Fang Xu 
Published: July 2020

Abstract
Oligosaccharides have important therapeutic applications. A useful route for oligosaccharides synthesis is reverse hydrolysis by β-glucosidase. However, the low conversion efficiency of disaccharides from monosaccharides limits its large-scale production because the equilibrium is biased in the direction of hydrolysis. Based on the analysis of the docking results, we hypothesized that the hydropathy index of key amino acid residues in the catalytic site is closely related with disaccharide synthesis and more hydrophilic residues located in the catalytic site would enhance reverse hydrolysis activity. In this study, positive variants TrCel1bI177S, TrCel1bI177S/I174S, and TrCel1bI177S/I174S/W173H, and one negative variant TrCel1bN240I were designed according to the Hydropathy Index For Enzyme Activity (HIFEA) strategy. The reverse hydrolysis with TrCel1bI177S/I174S/W173H was accelerated and then the maximum total production (195.8 mg/mL/mg enzyme) of the synthesized disaccharides was increased by 3.5-fold compared to that of wildtype. On the contrary, TrCel1bN240I lost reverse hydrolysis activity. The results demonstrate that the average hydropathy index of the key amino acid residues in the catalytic site of TrCel1b is an important factor for the synthesis of laminaribiose, sophorose, and cellobiose. The HIFEA strategy provides a new perspective for the rational design of β-glucosidases used for the synthesis of oligosaccharides.
Study on the community structure and function of symbiotic bacteria at different growth and developmental stages of Hypsizygus marmoreus
Authors: sun shujing , Li Fan , Xu Xin , Liu Yunchao , Kong Xuqiang , Chen Jianqiu , Liu Ting , Chen Liding 
Published: July 2020

Abstract
Background: The symbiotic bacteria associated with edible fungi are valuable microbial resources worthy of in-depth exploring. It is of significance to analyze the community structure and succession of symbiotic bacteria in edible fungi, which can also avail the isolating of growth-promoting strains that have an essential relationship with the cultivation cycle, and agronomic traits and yields of fruiting bodies.Results: In all the samples from cultivation bags of Hypsizygus marmoreus, 34 bacterial phyla were detected, and Firmicutes was the most abundant bacterial phylum (78.85%). The genus Serratia showed an exponential increase in abundance in the cultivation bags in the mature period, reaching a peak abundance of 55.74% and becoming the dominant symbiotic flora. The most dominant strain was Serratia odorifera HZSO-1, and its abundance increased with the amount of hyphae of H. marmoreus. Serratia odorifera HZSO-1 could reside in the hyphae of H. marmoreus, promote the growth and development, shorten the fruiting cycle by 3-4 days, and further increase the fruiting body yield by 12%. Conclusions: This study is a pioneering demonstration of the community structure of the symbiotic microbiota and bacteria-mushroom interaction in the growth and development of edible fungi. This work lays a theoretical foundation to improve the industrial production of mushrooms with symbiotic bacteria as assisting agents.
Development and Validation of Food Frequency Questionnaire for Food and Nutrient Intake of Adults in Butajira, Southern Ethiopia
Authors: Feyesa Ilili , Endris Bilal S , Habtemariam Esete , Hassen Hamid Y , Gebreyesus Seifu H 
Published: July 2020

Abstract
Background: To date, there is no culture-specific and validated Food Frequency Questionnaire (FFQ) available in Ethiopia. We developed a FFQ and evaluated its validity as compared to estimates of a food group and nutrient intakes derived from two 24-Hour Dietary Recalls (24-HRs).Method: The FFQ has a total of 89 food items. A total of 105 adults aged 20 to 65 years participated in this study. Wilcoxon- signed rank test, Spearman’s correlation, Cross-classification, kappa, and Bland-Altman analysis were used to validate food group intakes and nutrients derived from the FFQ against two 24-HRs.Results: Mean energy and macronutrient intakes obtained from the FFQ were significantly higher than those obtained from the mean of two 24-HRs. For energy and macronutrients, the correlation between two instruments ranged from 0.05 (total fat) to 0.32 (carbohydrate). Whereas, for micronutrients, it ranged from 0.1 (calcium) to 0.49 (vitamin B1). Visual inspection of the Bland-Altman plots for both energy and macronutrients shows no consistent trend across the intake values. For the majority of the food groups, no significant difference was observed in median intake of foods and nutrients between 24-HRs and the FFQ. The correlation coefficients for food groups ranged from 0.12 (egg) to 0.78 (legumes). The FFQ showed a fair classification agreement with the 24-HRs for cereals, legumes, and roots and tubers intake. A systematic trend of overestimation for roots and tubers and under estimation of beverage intakes at higher values was observed when we used FFQ.Conclusion: The FFQ is valid to assess and rank individuals in terms of intakes of most food groups according to high and low intake categories. However, estimates of roots and tuber and beverages should be interpreted with caution.
Novel strategy with the automatic non-coplanar volumetric-modulated arc therapy for angiosarcoma of the scalp.
Authors: Inui Shoki , Ueda Yoshihiro , Ohira Shingo , Tsuru Haruhi , Isono Masaru , Miyazaki Masayoshi , Koizumi Masahiko , Teshima Teruki 
Published: July 2020

Abstract
Background: Total scalp irradiation presents technical and dosimetric challenges. While reports suggest that HyperArc, a new stereotactic radiosurgery planning technique applied to non-coplanar volumetric-modulated arc therapy (VMAT) technique, is associated with high conformity and rapid dose fall-off, the performance of HyperArc for total scalp irradiation has not been explored. The current study aimed to compare the dosimetric performance of HyperArc plans with those of non-coplanar VMAT plans in angiosarcoma of the scalp. Methods: Ten patients with angiosarcoma of the scalp were included in this study. The performance of three different plans administered using TrueBeam Edge were compared: non-coplanar VMAT using flattening filter (FF) beams (VMAT-FF), HyperArc using FF beams (HyperArc-FF), and HyperArc using flattening filter free (FFF) beams (HyperArc-FFF). The dose distribution, dosimetric parameters, and dosimetric accuracy for each of these plans were evaluated. Results: The three plans showed no statistically significant differences in target volume coverage, conformity, and homogeneity. The HyperArc-FF and HyperArc-FFF plans provided significantly lower mean brain doses (12.63 ± 3.31 Gy and 12.71 ± 3.40 Gy) than did the VMAT-FF plans (17.11 ± 5.25 Gy). There were almost no differences in sparing the organs at risk between the HyperArc-FF and HyperArc-FFF plans. The HyperArc-FF and HyperArc-FFF plans provided a shorter beam-on time than did the VMAT-FF plan. The 3%/2 mm gamma test pass rates were above 95% for all three plans. Conclusions: Our results suggest that the HyperArc plan can be potentially used for radiation therapy of target regions with large and complicated shapes, such as the scalp, and that there are no advantages of using FFF beams.
Conservative management of CIN2p16 positive lesions in women with multiple HPV infection
Authors: Bruno Maria Teresa , Scalia Guido , Cassaro Nazario , Costanzo Maria , Boemi Sara 
Published: July 2020

Abstract
BackgroundAccording to the 2006 American Society for Colposcopy and Cervical Pathology guidelines, positive CIN2 p16 in women over the age of 25 should be managed with excisional treatment. However, excisional treatment is associated with physical, psychological and obstetric morbidity and can have a negative impact on sexual function. In our study we sought to identify a clear management strategy, addressing the impact of routine use of p16 immunohistochemistry in this population and identify appropriate criteria for patient selection with the aim of reducing over-treatment.MethodWe studied the medical records of 130 patients who had undergone laser therapy for CIN2. Each patient underwent colposcopy, biopsy and HPV test and were tested for p16 protein,. Patients were divided based on HPV infection into: single infections, multiple infections. All patients underwent ZTA laser therapy with follow-up (2-year follow-up ). Statistical analysis Contingency tables were created to evaluate the correlation between single, multiple and CIN2+ infections. Values with p <0.05 were considered statistically significant.ResultsSingle infections had a histological regression of 61.8% (21/34) and a histological persistence rate of 35.3% (12/34), which was greater than the multiple infection rate. The common characteristic that the women with persistence and progression had was the dimension of the lesion and the genotype 16. Ten cases of histological persistence and the only case of progression had one lesion greater than three quarters of the cervix.ConclusionsWith the progress of our understanding of the natural history of infection from human papillomavirus and the increasing use of colposcopy, thanks to the addition of HPV genotyping and the technique of immunohistochemistry, conservative management of these lesions is now possible.
Lenvatinib Promotes the Antitumor Effect of Doxorubicin in Anaplastic Thyroid Cancer
Authors: Su Xi , Liu Jiaxin , Zhang Haihong , Gu Qingqing , Zhou Xinrui , Ji Meiju , Yao Demao 
Published: July 2020

Abstract
Background: Anaplastic thyroid cancer (ATC) is a kind of rare thyroid cancer with very poor prognosis. It is one of the deadliest cancers in human due to the aggressive behavior and resistance to treatment. Doxorubicin has been approved in ATC treatment as a single agent, but monotherapy still shows no improvement of the total survival in advanced ATC. Lenvatinib was investigated with encouraging results in treating the patients with radioiodine-refractory differentiated thyroid cancer (DTC). However, antitumor efficacy of combination therapy with lenvatinib and doxorubicin remains largely unclear. Methods: The antitumor efficacy of combination therapy with lenvatinib and doxorubicin on ATC cell proliferation and was assessed by the MTT assay and colony formation. Flow cytometry were employed to assess ATC cells’ apoptosis and cell cycle arrest in response to combination therapy. Xenograft models were used to test its in vivo antitumor activity. Result: Lenvatinib monotherapy was less effective than doxorubicin in treating ATC cell lines and xenografts model. The combination therapy of lenvatinib and doxorubicin significantly inhibited ATC cell proliferation and tumor growth in nude mice, and induced cell apoptosis and cell cycle arrest in compared to lenvatinib or doxorubicin monotherapy. Conclusion: Lenvatinib promotes the antitumor effect of doxorubicin in ATC cell and xenografts model. Lenvatinib/doxorubicin combination may be a potential candidate therapeutic approach for anaplastic thyroid cancer.
Experiences with health care and health-related quality of life of patients with hematologic malignancies in Mexico
Authors: Doubova Svetlana Vladislavovna , Terreros-Muñoz Eduardo , Delgado-Lòpez Nancy , Montaño-Figueroa Efreen Horacio , Infante-Castañeda Claudia , Cuevas Ricardo Perez 
Published: July 2020

Abstract
Background: In Mexico, patients with hematologic malignancies (HMs) are characterized by being at high risk and advanced stages at diagnosis and by having a low cure rate; yet information on their experiences with health care and health-related quality of life (HRQL) is scarce. We aimed to evaluate experiences with health care and HRQL of patients with HMs and the association between these patient-reported measures. Methods: We conducted a cross-sectional survey in two public oncology hospitals in Mexico City. The study included outpatient cancer patients aged ≥18 years with a diagnosis of leukemia, lymphoma, or multiple myeloma. We used a patient-centered quality of cancer care questionnaire to assess patient experiences with receiving 1) timely care; 2) clear information; 3) information for treatment decision-making; 4) care to address biopsychosocial needs; and 5) respectful and coordinated care. We applied the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30) to measure HRQL. We performed a multiple linear regression to evaluate the association between patient-reported experiences (independent variables) and the QLQ-C30 summary score (dependent variable). Results: Of the 515 participating HM patients, 46.6% had lymphoma, 34% leukemia, and 19.4% multiple myeloma; 70.9% were at advanced stages or at high risk. Additionally, 15.1% had anxiety and 12.8% had depression. Over one third (35.9%) reported receiving clear information, 28.5% timely care, 20.6% information for treatment decision-making, 23.7% care that addressed their biopsychosocial needs, and 31% respectful and coordinated care. The mean QLQ-C30 summary score was 71.9 points. Timely care, clear information, and care that addresses biopsychosocial needs were associated with higher HRQL. Conclusions: Health care services for HM patients at public oncology hospitals in Mexico need improvement. Notably, providing timely care, clear information, and care that addresses patients’ biopsychosocial needs can increase the likelihood of better HRQL. Health care providers should measure and improve the experiences of HM patients with health care.