bluuebunny/crossref_metadata_2025_split huggingface dataset:
IgE reactivity to fish allergens from Pacific cod (Gadus macrocephalus) in atopic dogs
Authors: Imanishi Ichiro , Uchiyama Jumpei , Mizukami Keijiro , Kamiie Junichi , Kurata Keigo , Iyori Keita , Fujimura Masato , Shimakura Kuniyoshi , Nishifuji Koji , Sakaguchi Masahiro 
Published: September 2020

Abstract
Background: IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude extracts and purified allergens derived from the Pacific cod (Gadus macrocephalus) in atopic dogs to identify the allergenic proteins of cod.Results: The levels of specific IgE to crude cod extracts were measured in the sera of 179 atopic dogs, including 27 dogs with cod allergy, using enzyme-linked immunosorbent assay (ELISA). Specific IgE to crude cod extracts were present in 36 (20%) of the 179 atopic dogs and in 12 (44%) of the 27 dogs with cod allergy. The allergens in crude cod extracts were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that tested positive for specific IgE to crude cod extracts. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs tested positive for specific IgE to parvalbumin, collagen, and tropomyosin, respectively.Conclusions: The IgE reactivity to cod allergens observed in dogs was similar to that in humans, and this finding further supports the use of atopic dogs with fish allergy as a model for fish allergy in humans.
Functionally enhanced placenta-derived mesenchymal stem cells inhibit adipogenesis in orbital fibroblasts with Graves’ ophthalmopathy
Authors: Kim Jae Yeon , Park Sohae , Lee Hyun-Jung , Lew Helen , Kim Gi Jin 
Published: September 2020

Abstract
Background: Placenta-derived mesenchymal stem cells (PD-MSCs) have unique immunomodulatory properties. Phosphatase of regenerating liver-1 (PRL-1) regulates the self-renewal ability of stem cells and promotes proliferation. Graves’ ophthalmopathy (GO) is an autoimmune inflammatory disease of the orbit and is characterized by increased orbital levels of adipose tissue. Here, we evaluated the therapeutic mechanism for regulation of adipogenesis by PRL-1-overexpressing PD-MSCs (PD-MSCsPRL-1, PRL-1+) in orbital fibroblast (OF) with GO patients.Methods: PD-MSCs isolated from human placenta were transfected with the PRL-1 gene using nonviral transfection method. Primary OFs were isolated from orbital adipose tissue specimens from GO patients. After maturation as adipogenic differentiation, normal and GO-derived OFs were cocultured with naïve and PD-MSCsPRL-1. We analyzed the protein levels of adipogenesis markers and their signaling pathways in OF from GO patients. Results: The characteristics of PD-MSCsPRL-1 were similar to those of naïve cells. OFs from GO patients induced adipocyte differentiation and had significantly decreased a lipid accumulation after coculture with PD-MSCsPRL-1 compared to naïve cells. The mRNA and protein expression of adipogenic markers was decreased in PD-MSCsPRL-1. Insulin-like growth factor-binding proteins (IGFBPs) secreting PD-MSCsPRL-1 downregulated the phosphorylated PI3K/AKT/mTOR expression in OFs from GO patients. Interestingly, IGFBP2, -4, -6, and -7 expression in PD-MSCsPRL-1, which was mediated by integrin alpha 4 (ITGA4) and beta 7 (ITGB7), was higher than that in naïve cells and upregulated phosphorylated FAK downstream factor.Conclusion: In summary, IGFBPs secreting PD-MSCPRL-1 inhibit adipogenesis in OFs from GO patients by upregulating phosphorylated FAK and downregulating PI3K/AKT/mTOR signaling pathway. The functional enhancement of PD-MSCs by nonviral gene modification provides a novel therapeutic strategy for the treatment of degenerative diseases.
Optimal regimens regimens based on PK/PD cutoff evaluation of ceftiofur against Actinobacillus pleuropneumoniae in swine
Authors: Sun Da , Mi Kun , Hao Haihong , Xie Shuyu , Chen Dongmei , Huang Lingli 
Published: September 2020

Abstract
Background Actinobacillus pleuropneumoniae formerly known as Haemophilus pleuropneumoniae, can cause pleuropneumoniae in pigs, which lead to significant mortality. Ceftiofur was the first cephalosporin antibiotic used in animals，which was effective against gram-negative and gram-positive bacterium. This study aimed to formulate a rational dosage strategy and review the preceding recommended dosage based on PK/PD modeling and Establish Clinical breakpoint of ceftiofur against Actinobacillus pleuropneumoniae based on the pharmacodynamic-pharmacokinetic cutoff. Results The epidemiologic cutoff value was 0.125 μg/mL. The results of the pharmacodynamic study showed that the MICs of BW39 were 0.5 μg/mL and 1 μg/mL in vitro and ex-vivo, respectively. The minimal bactericidal concentrations (MBCs) under in vitro and ex vivo conditions were both 1 μg/mL. The time-killing profiles of ceftiofur against BW39 were time-dependent with a partly concentration-dependent pattern. Based on the inhibitory sigmoid Emax model, the AUC24 h/MIC values for the bacteriostatic, bactericidal, and elimination effects in serum were 45.73, 63.83, and 69.04 h for healthy pigs separately. According to the Monte Carlo simulation, the COPD was calculated as 2 μg/mL, and the optimized dosage regimen of ceftiofur against Actinobacillus pleuropneumoniae to achieve bacteriostatic, bactericidal, and elimination effects over 24 h was 2.13, 2.97, and 3.42 mg/kg for the 50% target attainment rate (TAR) and 2.47, 3.21, and 3.70 mg/kg for the 90% TAR respectively.Conclusions In conclusion, we reveal the EOFF and PK/PD cutoff values of ceftiofur against A. pleuropneumoniae in piglets. However, with the paucity of clinical data for ceftiofur to establish a clinical cutoff against A. pleuropneumoniae, the PK/PD cutoff value of 2 μg/mL will be recommended as surrogate. According to the PK/PD data and the MIC distribution in China, the single bactericidal dose was 3.21 mg/kg for the 90% target, which would be more able to cure Actinobacillus pleuropneumoniae and avoid the emergence of resistance for clinical ceftiofur use in piglet.
HIV-1 infection among women in Israel, 2010-2018
Authors: Wagner Tali , Olshtain-Pops Karen , Wax Marina , Horwitz Olivia , Shirazi Rachel , Gozlan Yael , Volnowitz Hadar , Mendelson Ella , Levy Itzchak , Mor Orna 
Published: September 2020

Abstract
Introduction: Although women comprise 33% of the HIV-1-carriers in Israel, they have not previously been considered a risk group requiring special attention. Immigration waves from countries in Africa and in East Europe may have changed the local landscape of women diagnosed with HIV-1. Here, we aimed to assess viral and demographic characteristics of HIV-1-positive women identified in Israel between 2010 and 2018.Methods: All >16 year-old, HIV-1-infected women, diagnosed in Israel in 2010-2018, (n=763) registered in the National HIV reference laboratory were included in this cross-sectional study. Demographic and clinical characteristics were extracted from the database. Viral subtypes and transmitted drug resistance mutations (TDRM) were determined in 337 (44.2%) randomly selected samples collected from treatment-naive women. Results: Median age at diagnosis was 38 years. Most (73.3%) women were immigrants from the former Soviet Union (FSU) (41.2%, 314) or sub-Saharan Africa (SSA) (32.2%, 246) and carried subtype A (79.7%) or C (90.3%), respectively. Only 11.4% (87) were Israeli-born women. Over the years, the prevalence of women from SSA decreased while that of women from FSU increased significantly (p<0.001). The median CD4+ cell count was 263 cells/mm3, and higher (391 cells/mm3) in Israeli-born women. TDRM were identified in 10.4% of the tested samples; 1.8%, 3% and 7.1% had protease inhibitors (PI), nucleotide reverse transcriptase inhibitors (NRTI) and non-nucleoside reverse transcriptase inhibitors (NNRTI) TDRM, respectively. The prevalence of women with NNRTI TDRM significantly increased from 4.9% in 2010-2012 to 13.3% in 2016-2018. Israeli-born women had the highest prevalence (16.3%) of NNRTI TDRM (p=0.014). NRTI A62 (5.6%), NNRTI E138 and K103 (5.6% and 4.2%, respectively) were the most prominent mutated sites. Conclusions: Most HIV-1-positive women diagnosed in Israel in 2010-2018 were immigrants, with the relative ratio of FSU immigrants increasing in recent years. The high proportion of women diagnosed with resistance mutations, particularly, the yearly increase in the frequency of NNRTI mutations, support the national policy of resistance testing at baseline.
A retrospective cross-sectional study of delayed closure of anterior fontanelle in healthy infants and its associated factors
Authors: Tang MengYan , Xiong Fei , Li Ping , Zeng Limin , Yang SuFei 
Published: September 2020

Abstract
Background: Anterior fontanelle (AF) is a regular feature of developing infants, and its delayed closure (AFDC) is highly valued in clinical child healthcare. Methods: A retrospective cross-sectional study was conducted with a 3-year follow-up to understand the occurrence of AFDC in healthy infants and its associated factors.Results: 61 out of 792 infants examined had AFDC, resulting in an incidence rate of 7.71% of AFDC in healthy infants. 92.29% of infants were found to have experienced AF closure by the age of 24 months, while up to 99.87% of them achieved closure before 36 months. From the age of 1 month to 36 months, the median AF size of the AFDC group was significantly larger than that of the normal closure (AFNC) group. Between 6 and 36 months, the median weight as well as length/height of the AFDC infants were both lower than those of their counterparts. Additionally, there indicated no difference in head circumference between two groups. Several factors were associated with AFDC in healthy infants, which were heavier birth weight (OR=1.001), longer birth length (OR=0.778), larger AF size at 1 month (OR=4.196), and being male (OR=1.851).Conclusion: AFDC in healthy infants was considered within a normal range for AF development and thus not pathological in this case. AF development was correlated to infant weight as well as length/height, but had no significant relationship with head circumference. Congenital factors such as birth weight, birth length, AF size at birth, and gender were found associated with AFDC in healthy infants.
The Recommended Online Social Networks (OSN) and Big Data System Using Large Scale Graph Partitioning with Mahout and PowerGraph
Authors: Gaol Ford Lumban , Matsuo Tokuro 
Published: September 2020

Abstract
Introduction : Social Big data is generated by interactions of connected users on social network. Sharing of opinions and contents amongst users, reviews of users for products, result in Social Big data. If any user intends to select product such as movies, books etc. from e-commerce sites or view any topic or opinion on social networking sites, there are a lot of options and these options result in information overload. Case Description : Social recommendation systems assist users to make better selection as per their likings. Recent research works have improved recommendation systems by using matrix factorization, social regularization or social trust inference. Furthermore, these improved systems are able to alleviate cold start and sparsity but not efficient for scalability. Discussion and Evaluation: The main focus of this paper is to improve scalability and provide better recommendations to users with large-scale data in less response time. We have partitioned social big graph and distributed it on different nodes based on Mahout and PowerGraph like system. Conclusion : In our approach, partitioning is based on direct as well as indirect trust between users and comparison with state-of-the-art approaches proves that statistically better partitioning quality is achieved using our approach. In our proposed approach ScaleRec, hyperedge and transitive closure are used to enhance social trust amongst users. Experiment analysis on standard datasets proves that better locality and recommendation accuracy is achieved by using our proposed approach.
Is teachers’ depression contagious to students? A study based on classes’ hierarchical models
Authors: Wu Wenfeng , Lu Yongbiao , Wang Ya 
Published: September 2020

Abstract
BackgroundAccording to the theory of emotional contagion, emotions in one person can trigger similar emotions in groups within social networks. In schools, the class just like a small social network, that teachers’ emotion, such as depression, might be contagious to their students. However, until now there is few studies reporting this issue. This study aims to determine whether teachers’ depression be contagious to students and what mechanics behind the phenomenon.MethodsUsing Children’s depression and cognitive scales to assess 2579 students, meanwhile using teachers’ depression and emotional labor scales assess 529 teachers. The nested data from 112 classes were analyzed.ResultsTeachers’ depression was positively correlated with emotional labor surface and deep acting, and teachers’ depression cross-level predicted students’ depression inversely. For teachers with higher levels of depression, the teacher’s deep acting affected their students’ depression significantly, the more effortful the teachers’ deep acting, the lower the degree of the students’ depression, however, for teachers with lower levels of depression, the deep acting was not significant.ConclusionThe results maybe state that depression in teachers is not readily transmitted to students, one of reasons is that teachers’ emotional labor may alleviate the influence of their depression on students. However, considered that teachers' emotional labor was positively correlated with their depression, the teachers’ emotional labor may be like a double-edged sword, while alleviating the influence of teachers' depression on students, it also deteriorated their own depression, making it impossible sustainable. For students’ depression interventions based in school, including teachers maybe a better selection.Trial registrationThis article is not a interventional research, so it was not registered.
Assessment for Medical students’ perception on feedback at department of surgery, School of Medicine and Dentistry in Korle-Bu Teaching Hospital
Authors: Kudzi William , Adu-Aryee Nii Adu , Sheriff Mohammed Albezel , Bannerman-Williams Emmanuel , Cobbinah Joseph Ezale , Nartey Edmund Tetteh 
Published: September 2020

Abstract
Background

Giving and receiving feedback are critical skills and should be part of the training of professionals in the medical field. Achieving the best from any medical training being offered requires consultants to adopt the best procedures and techniques. Feedback is given as part of assessment for learning or of learning, and it helps consultants to identify students’ difficulties and provide the needed support that would help improve upon the training being provided. depending on the nature of medical training and how consultants wish to support their students. The study investigated whether feedback given to students during their clinical training at the Surgical Department in Korle-Bu Teaching Hospital has been beneficial to trainees.
Methods

This was a cross sectional study using a questionnaire to determine the perceptions and knowledge of students in clinical training programme on the effectiveness of feedback received during their clinical training and whether they helped to improve their learning. Descriptive statistics were used to interpret the responses of the students, with mean values being calculated.
Results

A total of 448 students participated in the study with 50.9% (n = 228) being female and 49.1% male. Majority of participants (97.0%, n = 424) recognized feedback as an evaluative process and 78% (n = 340) had received feedback from lecturers. More than half of participants said there was no clear mechanism that is followed to give feedback to students (64%, n = 286). Majority of participants (38.7%, n = 170) were divided in their opinions as to whether feedback they receive was adequate, beneficial and provided opportunity for learning and improvement
Conclusion

Oral feedback was occasionally given to trainees by their lecturers. Feedback given does not follow any guidelines so the trainees did not really appreciate the real benefits of feedback. From this study, feedback was observed to be very beneficial, but trainees do not really benefit from the feedback given by their lecturers, because the manner in which is given does not promote effective teaching and does not also promote learning and students acquisition of skills on the ward during clinical rotation of trainees.
Clinical observation and analysis of patients with idiopathic membranous nephropathy combined with low 25(OH)D3 levels
Authors: Zou Fan , Huang Yu , Yang Zhenhua 
Published: September 2020

Abstract
Background: To investigate correlations between 25-hydroxyvitamin D3 [25(OH)D3] levels and selected clinical indicators and renal pathology at initial onset of idiopathic membranous nephropathy (IMN) and to analyse changes in the main quantifiable indicators in IMN patients with different 25(OH)D3 levels.Methods: A total of 109 patients admitted to the Department of Nephrology of the First Affiliated Hospital of Guangxi Medical University between August 2015 and April 2019 and diagnosed with IMN were reviewed. Comparative analysis of the effects of different 25(OH)D3 levels on clinical and pathological indicators of IMN was performed, and changes in serum albumin, the estimated glomerular filtration rate (eGFR), serum creatinine and other clinical indicators in these patients during the follow-up were analysed.Results: The 109 patients with IMN had 25(OH)D3 deficiency [25(OH)D3<70 nmol/L]. The incidence of nephrotic syndrome, serum phosphorus levels, high-sensitivity C-reactive protein (hs-CRP) levels, and erythrocyte sedimentation rates (ESRs) were higher in the 25(OH)D3<25 nmol/L group than in the 25(OH)D3≥25 nmol/L group. In contrast, total protein (TP), albumin (ALB), serum calcium, and IgG levels were lower in the 25(OH)D3<25 nmol/L group (P<0.05). No pathological parameters differed significantly between the two groups (P>0.05). Bivariate correlation analysis showed that 25(OH)D3 was positively correlated with nephrotic syndrome, TP levels, ALB levels, the eGFR, serum calcium levels and IgG levels (P<0.05) and negatively correlated with age, blood pressure, Scr levels, 24-hour urinary protein (UP) quantitation, serum phosphorus levels, hs-CRP levels and the ESR (P<0.05). In single-factor repeated measures analysis of variance, the ALB levels in the 25(OH)D3<25 nmol/L and 25(OH)D3≥25 nmol/L groups showed an increasing trend but were lower in the 25(OH)D3<25 nmol/L group than in the 25(OH)D3≥25 nmol/L group at each follow-up time point (P<0.001).Conclusion: Reduced 25(OH)D3 levels are prevalent among IMN patients. During the follow-up, serum albumin increased significantly in patients receiving vitamin D supplementation. 25(OH)D3 may play an important role in IMN development.
Frequency of Fabry Disease in a Juvenile Idiopathic Arthritis Cohort
Authors: Paim-Marques Luciana , Cavalcante Amanda Virginia , Verçosa Islane , Carneiro Paula , Souto-Maior Marcia , Marques Erlane , Appenzeller Simone 
Published: September 2020

Abstract
Background: Fabry disease (FD) is a rare, X-linked, multisystemic lysosomal storage disorder (LSD) that results from a deficiency in the hydrolase alpha-galactosidase A (⍺-GalA). In childhood, classic FD symptomatology is rare. Majority of children present with mild non-specific symptoms, including the musculoskeletal system. The prevalence of FD among juvenile idiopathic arthritis (JIA) patients is unknown. Objective: The aim was to identify the frequency of FD in a JIA cohort, characterizing early clinical symptoms, enzyme titers and GLA genotyping. Methods: Children with JIA followed in tertiary Children Hospital cohort were selected. Clinical, laboratorial, and familiar information was recorded. Molecular genetic testing to detect GLA gene mutations was performed in the girls and enzymatic analysis in the boys. Results: In 89 patients (56.2% female, age at disease onset: 8.93 ± 4.35 years), one male (1.12%) patient presented pathogenic mutation in GLA gene, c.1244T>C p.L415P, one female patient had a variant of uncertain significance c.38C>T (p.Ala13Val). The enzymatic activity of alpha galactosidase was slightly decreased in 3 additional (3.4%) patients. We observed the presence of intronic variants in 44.44% of our cohort: c.1000-22C> T; c.370-81_-77del; c.640-16A> G; c.10C>T; c.548-125C> G and c.-12G> A. These variants and their combination were associated with clinical symptoms in our cohort. Conclusions: The incidence of FD in our cohort was 1.12%. Intronic variants were associated with symptomatology described in the literature. Screening for FD in JIA may be a reasonable strategy for those with atypical pattern pain.
Optimization of the Novel Bisulfite Steeping Method by Response Surface Methodology for Natural Starch Synthesis From Oil Palm Trunk Biomass
Authors: Ahmed Zaber , Yusoff Mohd Suffian , Kamal Nurul Hana Mokhtar , Aziz Hamidi Abdul 
Published: September 2020

Abstract
Malaysia is the 2nd largest in palm oil export, and after overcoming economic age (average 25 years), the palm oil trees entail to replace usually. Therefore, a massive quantity of palm oil trunk biomass, containing a significant amount of starch, is available as bio-waste annually. The efficient extraction of this starch (carbohydrate polymer) would be worthwhile concerning the environment, economy, conversion of biowaste to bioresources, and waste dumping challenges. Central composite design executed an experimental model design, evaluated the impacts of process variables and their interaction through response surface methodology to optimize the novel bisulfite steeping method for starch synthesis. Design-Expert software performed the data analysis. The developed quadratic models for four factors (Strength of Sodium bisulfite solution, steeping hour, mixing ratio with the bisulfite solution and ultra-pure water) and one response (%Yield), demonstrated that a significant starch yield (13.54%) is achievable utilizing 0.74% bisulfite solution, 5.6 steeping hours, for 1.6 and 0.6 mixing ratio with the bisulfite solution and ultra-pure water respectively. Experimental outcomes were quite consistent with the predicted model, which eventually sustains the significance of this method. Malvern Zetasizer test revealed a bimodal granular distribution for starch, with 7.15µm of hydrodynamic size. Starch morphology was determined by scanning electron microscopy. X-ray diffraction investigation exhibits an A-type model, specifying persistent characteristics of extracted starch.