bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSX-40 Late-Breaking Abstract: Effect of Dietary Replacement of Fishmeal by Insect Meal on Growth Performance and Non-specific Immunity of Growing Pigs
Authors: Nekrasov Roman V , Ivanov Gennady A , Chabaev Magomed G , Tsis Elena Y , Bogolyubova Nadezhda V , Nikanova Daria A 
Published: November 2020

Abstract
Recent data show that dried larvae of darkling beetles (Zophobas morio L., DZML) and dried larvae of wax moths (Galleria mellonella L., DGML) contains a significant amount of protein, fat, chitin, melanin, antimicrobial peptides, trace elements etc. Larva’s fat is rich in lauric and other medium-chain fatty acids. The aim of our research was to study the influence of dietary replacement of fishmeal (FM) by insect meal on growth performance and nonspecific immunity of growing pigs. Experiments were performed using crossbred [(BWxL)xD] pigs (BW1=14.39±0.19 kg, N=27, n = 9) during the growing period. Animals were allocated to 3 groups: 1 – control (standard feed (SF), including FM), 2 – experimental (SF+2.5%DZML), 3 – experimental (SF+3.0%DGML). The materials obtained in the experiment was biometrically processed using the ANOVA method (at P &gt;0.05 or P &lt; 0.05). The use of DZML&DGML in feed didn’t reduce ADG of experimental animals during the growing period (413.23&413.76 vs. 413.76 g) compared to the control group. Dried larvae supplementation didn’t reduce feed intake and nutrient digestibility. The fat digestibility increased in the 2&3-experimental group (45.0&44.36 vs 29.75%, P &lt; 0.05), due to the high level of lipids and the profile of fatty acids in the larvae. Biochemical blood test showed a higher ALB/GLB ratio (0.85&1.43 vs 0.59, P = 0.05), higher number of WBC (12.36&12.89 vs. 10.44, P = 0.19) in the blood of the experimental animals compared to the control group. Growing pigs of the experimental groups had a higher level of nonspecific immunity, which is expressed in an increase in bactericidal, lysozyme activity of blood serum and phagocytic activity of neutrophils. Consequently, it is possible to consider the prospects of using DGML&DGML as dietary supplements to diets of growing pigs. The work was supported by the GZ АААА-А18-118021590136-7 of the Ministry of Science and Higher Education of the Russian Federation.
PSX-39 Late-Breaking Abstract: Characterization of epigenetic and transcriptional landscape in heat stressed rats using ATAC-seq and RNA-seq
Authors: Dou Jinhuan , Schenkel Flavio , Yu Ying , Wang Yachun 
Published: November 2020

Abstract
Understanding animal physiology and identifying reliable biomarkers may help to establish effective management strategies for the prevention of heat stress (HS). However, little is known about the molecular mechanism of mammal tolerance to high temperatures. In a previous study with Sprague-Dawley rats, we performed RNA-seq assays on the liver of rats in control (CT; 22 ℃, n = 5) and heat stress (HS120; 42 ℃ for 120 min, n = 5) groups. A total of 3,909 differential expression genes (DEGs, Q &lt; 0.05) were observed. This study was conducted to further examine the epigenetic landscape in the liver of rats under HS and identify transcription factors (TFs), as well as their regulated genes. Three liver tissues were selected from the RNA-seq samples and performed an Assay for Transpose Accessible Chromatin (ATAC-seq). Peaks meeting criteria of P&lt; 0.05 and |Fold Change| &gt;1.5 were considered as differential peaks. All ATAC-seq libraries generated an expected distribution of the insert fragment lengths, with the majority of fragments being small, which characterize inter-nucleosomal open chromatin, and progressively fewer fragments of larger size, which are spanning nucleosomes. The accessibility of transcriptional start sites (TSS) was significantly enriched. After merging data, 2,356 differential peaks showed CT having more accessible TSS than H120 and only 230 differential peaks showed H120 group having more accessible TSS than CT. Thirty-six and 22 TF motifs were predicted by up- and down-regulated differential peaks in H120 vs. CT. Together with the previous DEG results, we proposed candidate TFs annotated to Cebpa, Foxa4, and Sp3 DEGs, which are involved in the regulation of oxidative stress. In summary, we showed that nuclear chromatin in the liver of heat stressed rats was less open than that of control rats. We suggest that the TFs (Cebpa, Foxa4, and Sp3) may be involved in the physiological regulation of HS.
PSV-27 Program Chair Poster Pick: Expression QTL mapping for meat quality in beef cattle
Authors: Mateescu Raluca G , Leal-Gutiérrez Joel , Elzo Mauricio 
Published: November 2020

Abstract
Expression QTL mapping provides information about genetic variant with modulatory effects on gene expression which are useful for understanding the genetic architecture of complex phenotypes. This mapping allows for uncovering of genomic regions associated with transcription regulation of genes which can be related to phenotypic variation when they colocalize with QTLs (cis and trans effects), providing a molecular basis for the phenotype-genotype association. The objectives of the present research were to perform eQTL mapping for meat quality traits in longissimus dorsi muscle and to uncover genes whose expression is influenced by local or distant genetic variation. A total of 120 steers from the University of Florida Beef Unit multibreed Angus-Brahman herd born between 2013 and 2014 were used in this study. The first three principal components from a principal component analysis for all meat quality phenotypes were used to construct a meat quality index. Eighty animals were selected based on extreme meat quality index for mRNA sequencing and 100 bp paired-end reads were mapped against to the Btau_4.6.1 reference genome. eQTL mapping was performed using 112,042 SNPs and 8,588 genes. A cis QTL was defined as an SNP located no more than 1 Mb upstream of the transcription start site or downstream of the transcription end site of an annotated gene. Polymorphisms associated with expression of at least 20 genes in the case of eQTL were considered hot spots. The harboring or adjacent gene was defined as master regulators. Multiple cis eQTLs and sQTLs effects were identified and genes such as LSM2, SOAT1, TTN and TEK are a few examples of potential expression and splicing regulatory genes. A total of 27 expression and 13 splicing master regulator genes were uncovered, mainly cytoskeletal or membrane-associated proteins, transcription factors and DNA methylases.
PSV-26 Effect of feeding regime on meat quality of elk deer (venison) loin during aging
Authors: Lee Jinwook , Jang Aera , Kim Hye-Jin , Kim Hee-Jin , Kim Kwan-Woo , Roh Hee-Jong , Lee Sung-Soo , Lee Sang-Hoon 
Published: November 2020

Abstract
The objective of this study was to investigate the effects of feeding regimes on chemical composition, meat quality, and fatty acids composition of elk deer loin (EDL) during aging at 4°C. Sixteen 3-year-old elk hinds were randomly assigned to one of two dietary treatments, grazing pasture (GR) and barn feeding (BF). Eight elks were grazed on pasture with supplementary feed of 1.0% of body weight and the other eight elks were fed 1.0% of concentrate with ad libitum hay for 5 months. After slaughtering, EDL was dissected and aged for 56 days at 4°C in vacuum packaging. Proximate composition, physicochemical characteristics, and fatty acid composition of EDL were determined. Proximate composition of EDL showed no significant difference between feeding regimen and aging period. The pH values of EDL increased with increase of storage days (P &lt; 0.05). Drip loss increased by day 14 compared to initial day and shear force was decreased during aging (P &lt; 0.05). Water holding capacity (WHC) and cooking loss were not significantly affected by feeding regimen or aging period. Lightness (L*) and redness (a*) were decreased on day 56 compared to initial day. Yellowness (b*) of EDL decreased with increase of aging time (P &lt; 0.05). The α-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid were higher in GR treatment and increased with aging duration (P &lt; 0.05). The feeding regimens and aging affected on meat quality and fatty acids profiles.
PSV-31 Influence of Hempseed Meal Supplementation on Fresh and Cooked Characteristics of Boer Cross Goats
Authors: Smith Hunter R , Abrahamsen Frank W , Rehm John G , Wilborn Barney , Blythe Eugene , Sawyer Jason T , Gurung Nar 
Published: November 2020

Abstract
Hempseed meal is byproduct of hemp oil production and is relatively high in fiber, fat, and protein, making it a potential feedstuff for ruminants. However, the impact this new byproduct could have on meat characteristics is unknown. The objective of the current study was to evaluate the impact of HSM on fresh and cooked characteristics of Spanish meat goat retail cuts. Forty Boer crossbred castrated male goats were randomly allocated to one of four diets (n = 10; 0, 10, 20 or 30%) containing HSM which originated from the manufacturing industry of hemp oil. Goats were provided 2 kg of diet daily that contained varying levels of HSM in addition to ad-libitum access to water throughout the study. Following a 60-day feeding period goats were harvested at the Lambert-Powell Meats Laboratory located at Auburn University. After chilling for 12 h at 0°C, carcass measurements were collected prior to carcass fabrication. Goat carcasses were fabricated into wholesale cuts of the shoulder, rack, loin and leg. Paired loins and legs were sliced into 2.54-cm-thick chops, vacuum packaged, and assigned to cook yield, instrumental fresh color, and instrumental tenderness and frozen at -23°C until all laboratory analysis could be completed. The use of HSM did not alter cooking yield in either the goat loin or leg chop (P &gt; 0.05) regardless of inclusion percentage within the diet. Furthermore, Warner-Bratzler shear force (WBSF) did not differ (P &gt; 0.05) with increasing inclusion rates of HSM for either the loin or leg chops. Instrumental color for lightness (L*) or yellowness (b*) did not differ (P &gt; 0.05) across diet treatments for either the loin or leg chops. These findings suggest that the inclusion of HSM within the diet of Boer cross goats did not alter fresh or cooked meat characteristics in either the loin or leg chops.
PSXI-4 Beauveria bassiana to control the mealworm Alphitobius diaperinus in chicken barns
Authors: Nadeau Martin , Todorova Silvia , Julien Carl 
Published: November 2020

Abstract
One of the most common insects in poultry houses is the mealworm Alphitobius diaperinus, also known as the darkling beetle. Immature (larvae) and adults feed on seeds spilled on the ground or from poultry droppings. In addition to structural damage, this insect can also be a vector of diseases that can affect birds. In addition, the indigestible shell of the insect can cause digestion problems for birds, and thus affect the profitability of production. It is considered impossible to eliminate the populations of mealworms, and in organic poultry production, the control options are limited. The mycoinsecticide BioCeres WP, based on the fungus Beauveria bassiana, and which controls aphids, has been proposed to control the mealworm A. diaperinus in chicken barns. 450 adult mealworms from a commercial chicken barn were randomly distributed in 9 containers (1 m2) containing 10 cm of wood shavings litter and chicken feed. BioCeres WP was applied by spraying two applications of 40 ml of 10 g/L solution (representing 4 x 10^9 conidia per m2 per application). Mealworms were housed during a broiler batch at the chicken barn they were harvested from and received one of the following treatments: 1) control, water spraying; 2) BioCeres WP treatment. 3 repetitions for control and 6 for BioCeres WP were performed. BioCeres WP increased the mortality rate of mealworms (+101%, P &lt; 0.02; 17.8% vs. 8.6%), compared to untreated mealworms, 28 days after treatment. Mortality rates were affected from day 14 until day 28 after treatment. A trial using 640 broilers in 32 pens revealed no adverse effect on growth performance or health. In summary, BioCeres WP could be involved in a biological strategy to control the mealworm Alphitobius diaperinus in chicken barns.
PSXI-5 Best production practices for improvement of Beef Cattle carcass quality
Authors: Amaral Thais B , Fernandes Arthur Francisco Araujo , Rosa Guilherme J M 
Published: November 2020

Abstract
Best production practices for Beef Cattle (BPP) is a protocol created by Embrapa Beef Cattle in Brazil, to guide farmers towards more sustainable production. The protocol includes checkpoints to the whole production system (management, nutrition, animal health and welfare, pasture, reproduction, environmental, etc.). Despite the benefits of the BPP, it is not mandatory for Brazilian farmers. As such, until the present moment only a few farmers have adopted it. The aim of this work was to evaluate the impact of the BPP on the carcass classification of animals from farms registered in the Brazilian government incentive program “Precoce MS.” The program was created in the state of Mato Grosso do Sul - Brazil in 2017 and provides tax benefits to beef producers who harvest animals at young ages with superior carcass quality. Nowadays, almost a quarter of the total beef harvested in MS State is originated from this program. The bonus ranges from 49% up to 67% of tax refunds, depending on the carcass classification. We have analyzed data from all animals slaughtered from the beginning of 2017 until the end of 2018, with a total of 1,107 million animals from 1,437 farms. At the slaughterhouse, animals were submitted to carcass classification. To comply with the program requirements, carcasses should meet three basic criteria: age (maximum 4 permanent teeth), fat deposition (between 1 mm and 10 mm) and carcass weight (&gt; 225 kg for steers and bulls, and &gt; 180 kg for heifers). Farmers that adopted BBP had only 12.4% of disqualified animals compared to 16,1% from farms that did not adopt the protocol (P &lt; 0,001). We concluded that farms that follow the BPP protocol achieved significantly higher quality carcass, which should encourage more farmers to adopt the protocol in the future.
PSII-17 Program Chair Poster Pick: Effect of ruminal protozoa on methane emissions in ruminants – a meta-analysis
Authors: Dai Xiaoxia , Kalscheur Kenneth , Huhtanen Pekka , Faciola Antonio 
Published: November 2020

Abstract
The effects of ruminal protozoa (RP) concentration on methane emissions from ruminants were evaluated in a meta-analysis using 67 publications reporting data from 85 in vivo experiments. Experiments included in the database reported methane emissions (g/kg DMI) and RP (log10 cells/mL) from the same group of animals. Quantitative data including diet chemical composition, ruminal fermentation, total tract digestibility, and milk production; and qualitative information including methane mitigation strategies, animal type, and methane measurement methods were also collected. The studies were conducted in dairy cows (51%), beef steers (32%) and small ruminants (32%). 70% of the studies reported a reduction in methane emissions. Supplemental lipids reduced methane emissions 95% of the time. The relationship between methane emissions and RP concentration was evaluated as a random coefficient model with the experiment as a random effect and weighted by the inverse pooled SEM squared, including the possibility of covariance between the slope and the intercept. A quadratic effect of RP concentration on methane emissions was detected: CH4= -28.8 + 12.2 × RP-0.64 × RP2. To detect potential interfering factors in the relationship, the influence of several qualitative and quantitative factors were separately tested. Acetate, butyrate, and isobutyrate molar proportions had positive relationships with methane emissions and influenced the relationship between RP concentration and methane emissions, where the presence of ruminal fermentation variables reduced the effects of RP concentration in methane emissions. Total tract digestibility of DM, OM, and CP had negative relationships while NDF digestibility had a positive relationship with methane emissions; however, they only changed the magnitude of intercept and slope of RP and RP2 for the relationship. For dairy cows, milk fat and protein concentrations had positive relationships and milk yield had a negative relationship with methane emissions and changed the magnitude of intercept and slope of RP and RP2 for the relationship.
PSVI-4 Effects of exogenous fibrolytic enzymes on beef cattle fed growing diets: Ruminal microbiome
Authors: Silva Kaliu G Scaranto , Favero Igor Gomes , Nardi Kaue Tonelli , Kondratovich Lucas Barbosa , Hoffmann Carly A , Hinds Jordan K , Hall Nathaniel , Henry Darren D , Sarturi Jhones Onorino 
Published: November 2020

Abstract
The effect of pre-treatment with fibrolytic enzymes [cellulase/xylanase (Trichoderma ressie)] of growing diets (high quality and low-quality) on ruminal microbiome relative abundance (RA) were evaluated. Ruminally cannulated beef steers (n = 5; BW = 520 ± 30 kg) were used in a 5×4 unbalanced Latin square design using a 2×2 factorial arrangement of treatments: (a) growing diet quality [high (HQ) and low (LQ)] and (b) enzyme inclusion (0 or 0.75 mL/kg of diet DM). Steers were individually fed ad libitum throughout four 21-d periods consisting of 14-d of adaptation and 7-d of collections. Ruminal fluid samples (100 mL) were collected on d-5 of each collection-period, at 6 h post feeding for DNA extraction and determination of microbial RA. Microbiome data were sequenced by Illumnia® NovaSeq™ 6000 (16S rRNA). Regardless of enzyme×diet quality interaction (P ≥ 0.11) or pre-treatment with enzyme (P ≥ 0.12), Domain RA was affected (P ≤ 0.04), in which LQ diets increased RA of Bacteria (93.25 vs. 86.80%) and decreased Archaea (6.75 vs. 13.20%). In Phylum, LQ diets decreased RA (P ≤ 0.04) of Euryarchaeota (6.75 vs. 13.21%), and increased Bacteroidetes (11.22 vs. 2.26%). Within Class, LQ diets decreased RA (P ≤ 0.04) of Clostridia (38.66 vs. 51.40%), Methanobacteria (6.75 vs. 13.21%), and increased Bacteroidia (10.62 and 1.47%). Within Order LQ diets, decreased RA (P ≤ 0.04) of Clostridiales (38.47 vs. 51.29%), Methanobacteriales (6.75 vs. 13.21%), and increased Bacteroidales (10.62 vs. 1.47%). In Family, LQ diets showed decreased RA (P ≤ 0.04) of Methanobacteriaceae (6.75 vs. 13.21%), Ruminococcaceae (6.71 vs. 2.18%), and increased Prevotellaceae (9.83 vs. 1.17%). In Genus, LQ diets showed increased RA (P ≤ 0.03) of Prevotella (9.61 vs. 1.10%). The dietary pre-treatment with fibrolytic enzymes seems to not dramatically affect RA of ruminal microbiome, while growing diet quality greatly influenced ruminal microbiome RA.
PSVI-2 Effects of dietary supplementation of a Saccharomyces cerevisiae-based direct-fed microbial product on plasma carbonyl-metabolome and fecal bacterial community of beef steers
Authors: Ogunade Ibukun M , Compart Devan M , Pech-Cervantes Andres A 
Published: November 2020

Abstract
We examined the effects of dietary supplementation of a Saccharomyces cerevisiae-based direct-fed microbial on plasma carbonyl-metabolome and fecal bacterial community of beef steers during a 42-d receiving period. Forty newly-weaned beef steers were used in this study. The steers were stratified by BW and randomly assigned to receive a basal diet with no additive (CON; n = 20) or a basal diet supplemented with 19 g of CommenceTM (PROB; n = 20). CommenceTM (PMI, Arden Hills, MN) contains a blend of 6.2 × 1011 cfu/g of S. cerevisiae, 3.5 × 1010 cfu/g of a mixture of Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and L. casei. On d 0 and 40, rectal fecal samples were collected for bacterial community analysis via sequencing of the V3-V4 region of 16S rRNA gene. On d 42, blood was collected for analysis of carbonyl-containing metabolites in plasma using a chemical isotope labeling/liquid chromatography mass spectrometry-based untargeted metabolomics. The data were analyzed using the GLIMMIX procedure of SAS with treatment as a fixed effect) and (random effect). A total number of 812 unique plasma metabolites were detected. Up to 305 metabolites [fold change (FC) ≥ 1.5, FDR ≤ 0.01] including glucose, hippuric acid, glycoaldehyde, and 5-hydroxykynurenamine were increased by PROB supplementation, whereas 199 metabolites (FC ≤ 0.63, FDR ≤ 0.01) including acetoacetate were reduced. Correlation analysis showed that plasma concentrations of 5-oxopentanoate, 2-aceto-2-hydroxybutanoate, and 3-methyl-2-oxopentanoic acid were positively correlated (P ≤ 0.10) with average daily gain. Supplemental PROB increased (P ≤ 0.05) the relative abundance of Prevotellaceae, Megasphaera, Dorea, Acetitomaculum, and Blautia. In contrast, the relative abundance of Elusimicrobium, Moheibacter, and Stenotrophomonas were reduced (P ≤ 0.05). This study demonstrated that PROB altered the plasma carbonyl-metabolome and fecal bacterial community of the beef steers.
PSXII-4 Serum citrulline concentration of unweaned calves transported by road as a potential biomarker of gut functionality
Authors: Robles Jose Angel , Marti Sonia , Pisoni Lucía , Bassols Anna Maria , Devant Maria 
Published: November 2020

Abstract
In human’s serum citrulline concentration (SCC) have been proposed as a biomarker of small intestinal absorption. We hypothesize that the stress and feed restriction that unweaned calves suffer during transport impairs gut functionality reducing SCC. The aim of this study was to evaluate if road transport hours and adaptation period after arrival would affect SCC in unweaned calves. A total of 1,601 calves (3–4 wk of age; 65 ± 9 kg of BW) were transported with 8 trucks (n = 4 each journey): short transport (ST; &lt; 9 h), and long transport with a rest stop proving feed and water to the calves (LT; &gt; 9 h). A subset of 20 calves for each truck were sampled for SCC at arrival (t0), and 14 d after arrival (t14); in case of LT, also at arrival at the resting facility (t-1). SCC was analyzed using a commercial kit (intra- and inter-assay CV were 12.62% and 6.32%). Data was analyzed using a mixed model with journey hours and day as main effects, and calf and truck as random effects. SCC tended (P = 0.08) at t0 to be greater in LT (47.7 ± 3.39 mM) compared to ST (42.6 ± 2.46 mM). At t14 SCC increased in ST and LT calves; however, no differences were observed in SCC (55.9 ± 3.42 mM vs. 58.8 ± 2.52 mM for LT and ST, respectively). In LT calves rest stop did not affect SCC (43.2 ± 1.74 mM vs. 39.8 ± 1.76 mM for t-1 and t0, respectively), while at t14 (50.6 ± 1.86 mM) SCC was greater (P &lt; 0.001) than t0 and t-1. The effect of transport type and evolution after transport on serum plasma citrulline concentrations may suggest that it’s a potential indicator of gut functionality in unweaned calves.