bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myf6/MRF4 is a myogenic niche regulator required for the maintenance of the muscle stem cell pool
Authors: Lazure Felicia , Blackburn Darren M , Corchado Aldo H , Sahinyan Korin , Karam Nabila , Sharanek Ahmad , Nguyen Duy , Lepper Christoph , Najafabadi Hamed S , Perkins Theodore J , Jahani‐Asl Arezu , Soleimani Vahab D 
Published: October 2020

AbstractThe function and maintenance of muscle stem cells (MuSCs) are tightly regulated by signals originating from their niche environment. Skeletal myofibers are a principle component of the MuSC niche and are in direct contact with the muscle stem cells. Here, we show that Myf6 establishes a ligand/receptor interaction between muscle stem cells and their associated muscle fibers. Our data show that Myf6 transcriptionally regulates a broad spectrum of myokines and muscle‐secreted proteins in skeletal myofibers, including EGF. EGFR signaling blocks p38 MAP kinase‐induced differentiation of muscle stem cells. Homozygous deletion of Myf6 causes a significant reduction in the ability of muscle to produce EGF, leading to a deregulation in EGFR signaling. Consequently, although Myf6‐knockout mice are born with a normal muscle stem cell compartment, they undergo a progressive reduction in their stem cell pool during postnatal life due to spontaneous exit from quiescence. Taken together, our data uncover a novel role for Myf6 in promoting the expression of key myokines, such as EGF, in the muscle fiber which prevents muscle stem cell exhaustion by blocking their premature differentiation.
Long-term Outcomes of Individuals With Metabolic Diseases Identified Through Newborn Screening
Authors: Mütze Ulrike , Garbade Sven F. , Gramer Gwendolyn , Lindner Martin , Freisinger Peter , Grünert Sarah Catharina , Hennermann Julia , Ensenauer Regina , Thimm Eva , Zirnbauer Judith , Leichsenring Michael , Gleich Florian , Hörster Friederike , Grohmann-Held Karina , Boy Nikolas , Fang-Hoffmann Junmin , Burgard Peter , Walter Magdalena , Hoffmann Georg F. , Kölker Stefan 
Published: October 2020

BACKGROUND:
Although extended newborn screening (NBS) programs have been introduced more than 20 years ago, their impact on the long-term clinical outcome of individuals with inherited metabolic diseases (IMDs) is still rarely investigated.

METHODS:
We studied the clinical outcomes of individuals with IMDs identified by NBS between 1999 and 2016 in a prospective multicenter observational study.

RESULTS:
In total, 306 screened individuals with IMDs (115 with phenylketonuria and 191 with other IMDs with a lifelong risk for metabolic decompensation) were followed for a median time of 6.2 years. Although the risk for metabolic decompensation was disease-specific and NBS could not prevent decompensations in every individual at risk (n = 49), the majority did not develop permanent disease-specific signs (75.9%), showed normal development (95.6%) and normal cognitive outcome (87.7%; mean IQ: 100.4), and mostly attended regular kindergarten (95.2%) and primary school (95.2%). This demonstrates that not only individuals with phenylketonuria, serving as a benchmark, but also those with lifelong risk for metabolic decompensation had a favorable long-term outcome. High NBS process quality is the prerequisite of this favorable outcome. This is supported by 28 individuals presenting with first symptoms at a median age of 3.5 days before NBS results were available, by the absence of neonatal decompensations after the report of NBS results, and by the challenge of keeping relevant process parameters at a constantly high level.

CONCLUSIONS:
NBS for IMDs, although not completely preventing clinical presentations in all individuals, can be considered a highly successful program of secondary prevention.
Retinalis biomarkerek szerepe a vascularis dementia és az Alzheimer-kór korai formáinak diagnosztikájában
Authors: Élő Ágnes , Czakó Cecília , István Lilla , Benyó Fruzsina , Nagy Zoltán Zsolt , Kovács Illés 
Published: October 2020

Absztrakt:
A dementiák társadalmunkban egyre nagyobb problémát jelentenek, folyamatosan nő a
megbetegedések száma. A dementia leggyakoribb előfordulási formája az
Alzheimer-kór, mely az esetek 60–80%-áért felelős. Kórlefolyása hosszú, a
preklinikai stádiumtól a súlyos Alzheimer-kórig több év, évtized is eltelik. Az
Alzheimer-kór egy olyan progresszív neurodegeneratív betegség, amely súlyos
szellemi leépüléshez vezet. A diagnózishoz számos, kognitív funkciót mérő teszt
és képalkotó vizsgálat áll rendelkezésre, a korai diagnózis azonban nagyobb
kihívást jelent, így a betegség gyakran későn kerül felismerésre. Mivel az agyi
és a retinalis keringés között bizonyítottan kapcsolat van, felmerül annak
lehetősége, hogy a retina vizsgálata az Alzheimer-kór és a vascularis dementiák
korai diagnózisában és progressziójának követéséhez is segítséget nyújthat. Az
utóbbi években a retina képalkotó vizsgálataiban bekövetkezett fejlődésnek
köszönhetően lehetővé vált a retina strukturális és vérkeringési elváltozásainak
vizsgálata a dementiák különböző típusaiban. Ezek alkalmazása nemcsak a korai
diagnózisban, hanem a progresszió megítélésében és a terápia nyomon követésében
is segítséget nyújthat a jövőben. Orv Hetil. 2020; 161(41): 1744–1752.
Az életkorral arányos D-dimer-emelkedés és a SARS-CoV-2-fertőzött betegek eseti halálozása
Authors: Nagy Zsuzsanna , Vásárhelyi Barna , Vajda Zoltán 
Published: October 2020

Absztrakt:
Bevezetés: A COVID–19 kórlefolyásában újabban több szerző
felvetette a D-dimer-pozitivitás és a D-dimer-emelkedés prediktív értékét a
betegség súlyosbodásában, illetve az eseti halálozásban.
Célkitűzés: Magyar betegek értékeinek összehasonlítása a
nemzetközi adatokkal, ennek részeként a D-dimer prediktív értéke a 2 héten
belüli eseti halálozásra. Módszerek: A szerzők 51, RT-PCR-rel
igazolt SARS-CoV-2-fertőzött beteg D-dimer-eredményeit dolgozták fel
retrospektív módon. Megvizsgáltuk, hogy ha a D-dimer eléri vagy meghaladja a
vágóérték 4-szeresét, akkor ez milyen kockázatot (odds ratio) jelent a
halálozásra. Logisztikus regresszióval meghatároztuk, hogy a 2 héten belüli
halálozás esélyhányadosa a konvencionális vágóérték hányszorosa mellett lesz
szignifikáns. Eredmények: Az 51 betegből 13 halt meg a kórházba
kerülést követően 2 héten belül. Megállapítottuk, hogy a tradicionális, 0,5
µg/ml FEU vágóérték mellett meghatározott D-dimer-pozitivitásnak alacsony a
prediktív értéke a halálozásra. Ha azt vizsgáltuk, hogy a D-dimer eléri vagy
meghaladja a vágóérték 4-szeresét, és ez milyen kockázatot jelent a halálozás
szempontjából, akkor a logisztikus regresszió paraméterei 1,64-szeres emelkedést
mutattak (p = 0,00183), és az esélyhányados (odds ratio) értéke 5,17 (CI 95% =
1,32–20,22) volt. A D-dimer prediktív értéke a halálozásra az életkor
függvényében változik. A 13 elhunyt közül 12 idősebb volt 80 évesnél, így a 80
év feletti életkor a magas D-dimer-szintnél nagyobb kockázatot jelentett a
halálozásra, odds ratio: 20,7 (CI 95% = 2,41–175,5).
Következtetés: A több mint négyszeres vágóértékre
emelkedett D-dimer-szint az életkor mellett COVID–19-ben előre jelezheti a 2
héten belüli halálozást. Orv Hetil. 2020; 161(41): 1739–1743.
The use of the ICAM Splint Programme in Zone IV to VII Extensor Tendon Repairs: Patient outcomes and clinician experiences in a specialised hand unit in SA
Authors: Mottay Nicole D , Govender Pragashnie , Mpanza December Mandlenkosi 
Published: October 2020

Background: The ICAM splint programme was developed in the 1980s and is known to produce favourable results in the management of extensor tendon repairs from zone IV to zone VII. It has only become popular in South Africa over the past several years and to the authors' knowledge its application in South Africa has not previously been reported.Aim: The study sought to describe the use of the ICAM splint programme following an extensor tendon repair from zone IV to VII at a specialised hand unit in SA.Methods: The study followed a concurrent mixed methods design. In phase one, a retrospective file audit was conducted with a sample of patients following the ICAM programme (n=75). Phase two involved individual therapist interviews (n=3), surgeon interviews (n=2) and a focus group with physiotherapists and occupational therapists (n=7) that had experience with the ICAM splint programme.Microsoft excel was used to descriptively analyse phase one data and thematic analysis was used to analyse phase two data. Data were merged and represented in a joint display.Results: The majority of patients (83%) were male with 40% in the age range of 26-35 years old. The injuries were mainly violence-related (69%) in comparison to accidental injuries (31%) with extensor tendon zone V (40%) and VI (47%) being the most commontly involved. Adherence was reportedly influenced by factors such as patients' financial status, education and language barriers.Conclusions: The results suggest that the ICAM splint programme is beneficial in managing extensor tendon repairs from zone IV to VII. Therapists should consider factors that affect non-adherence when selecting patients for the ICAM programme to determine its suitability. Educating patients on the ICAM splint programme is essential to facilitate adherence. Novice therapists to the ICAM programme should receive formal training to improve confidence and skill.
Key words: ICAM splint programme, extensor tendon repair, zone IV to VII
Experiences of adults with psychiatric disabilities participating in an activity programme at a psychosocial rehabilitation centre in the Western Cape
Authors: Ramafikeng Matumo , Beukes Lisa , Hassan Aneeqa , Kohler Tara , Mouton Tayla Leigh , Petersen Saadiqah 
Published: October 2020

Introduction: Adults with psychiatric disabilities require continued community-based care to support their recovery and community reintegration. Community-based mental health services such as psychosocial rehabilitation day centres and group homes were to support community reintegration of these adults. However, these services are limited and opportunities for adults with psychiatric disabilities to engage in meaningful occupations that promote occupational well-being are scarce, particularly in South Africa.In addition literature exploring the experiences of adults with psychiatric disabilities using these services, is limited. The aim of the study was to describe the adults with psychiatric disabilities' experiences of participating in an activity programme at a psychosocial rehabilitation day centre.Method: A descriptive qualitative design was adopted, and eight participants were recruited through purposive sampling. Data were collected through observations and focus group discussions and analysed using thematic analysis.Findings: Two themes emerged: "What's nice about the activities that we do do, is it takes you out your head for a while and If you don't do anything [...] your brain goes that way a bit". The activities were valued as they offered opportunities for improved socialization and enabled participants to meet various occupational needs.Conclusion: Adults with psychiatric disabilities derived meaning, purpose and improved occupational well-being from participating in the activity programme. There is a need for more community-based mental health services to support the recovery of adults with psychiatric disabilities, thereby improving their quality of life within their communities.
Key words: psychiatric disability; occupational well-being; psychosocial rehabilitation day centre; community mental health services
Clinical practice in occupational therapy for treatment of concussion in schoolboys: An integrative review
Authors: Sinnett Faye , Franzsen Denise 
Published: October 2020

Rugby in South Africa is one of the fastest growing contact sports and schoolboys competing in rugby will sustain injuries such as concussions. Concussion protocol regulations indicate that young players be removed from play and training for two weeks as they are many misconceptions about the long-term implications of concussion. Concussion has been shown to impact on cognitive functional and the ability to perform at school. Occupational therapy has a role in the intervention of occupational performance post-concussion in learners playing this contact sport.A clinical practice framework to provide guidelines for the occupational therapy intervention for schoolboy rugby players post-concussion in the South African setting is presented. The framework was based on the occupations, client factors, skills, performance patterns and context and environments in the Occupational Therapy Practice Framework III (OTPF III). Screening assessment including the learners, parents and teachers were suggested to determine the use of specific occupational performance assessments in education, social participation, activities of daily living and rest and sleep as well asas client factor assessments to determine the outcomes of concussion.Intervention guidelines include the occupational therapy process approaches for Create/Promote, Establish/Restore, Maintain, Modify (compensate) and Prevent, with a focus on create/promote or promotion of safety in rugby.The assessments given in this framework are easily accessible in the South African setting and provide knowledge for all level of practitioners. The role of occupational therapy in reducing residual risks of concussion sustained while playing rugby at school and the long-term consequences of concussion on occupations needs to be researched further.
Key words: concussion, schoolboy concussion, concussion management, occupational therapy management
THE INTRA-ASSAY PRECISION AND TIME STABILITY OF QUANTITATIVE βIII-TUBULIN EXPRESSION ASSESSMENT IN SOLID TUMOR TISSUE
Authors: Basharina A. A. , Bogush T. A. , Rukavishnikova E. A. , Bogush E. A. , Kaliuzhny S. A. , Vikhlyantseva N. O. , Kosorukov V. S. 
Published: October 2020

Introduction. The introducing of tumor molecular profiling into clinical practice has revealed the need for development of new analytical methods for estimating marker expression in solid tumors, as routinely used method of immunohistochemistry has a number of significant drawbacks.Objective. Analytical validation of immunofluorescence staining and flow cytometry method developed by the authors for the examination of tumor protein markers in the solid tumors tissue.Materials and methods. Method validation was carried out by quantitative estimation of βIII-tubulin (TUBB3) expression in single-cell suspensions of non-small-cell lung cancer obtained from surgical tumor samples. Primary antibodies to TUBB3 (ab7751) and secondary DyLight 650-conjugated antibodies (ab98729) were used for immunofluorescent staining. The «Navios» flow cytometer (Beckman Coulter) was used to measure the fluorescence. The validation parameters were assessed by the coefficient of variation calculated as the ratio of standard deviation of TUBB3 level to its mean value.Results. Two parameters were analyzed: intra-assay precision and time stability of the results of the TUBB3 expression assessment. It was demonstrated that the mean coefficients of variation of the marker expression level in the tumor tissue did not exceed 20 % for both parameters. According to recommendations on the analytical validation of methods based on flow cytometry, it proves the validity of the method for these parameters.Conclusions. The intra-assay precision and time stability were demonstrated for the results of a quantitative estimation of TUBB3 expression in solid tumor tissue using immunofluorescence staining and flow cytometry method developed by the authors. The practical value of the time stability of immunofluorescence stain during 24 h storage of a stained cells suspension in the dark at 4 °C was highlighted. It shows the possibility of adjusting the time interval between completion of the analytical study part and flow cytometer measurement.
Pre and post training study of awareness about biomedical waste management among health care personnel in medical college hospital in Western Rajasthan
Authors: Kumar Soni Laxman , Soni Priyanka , Soni Ankita 
Published: October 2020

The biomedical waste is any waste that is generated during diagnosis, treatment or immunization of human beings or animals, or in the research activities of biological. The amount of biomedical waste being generated is increasing day by day which can pollute the environment and can spread many harmful diseases if not handled properly. Health care workers in our country are still not fully aware about proper disposal of BMW. The objective of the study was to access the effect of training on health care personnel about the biomedical waste management in our institution. : A pre designed questionnaire was used for data collection before and after hands on training. Total 250 health care personnel including doctors, nurses, lab technicians and class IV employees working in our institution participated in study. Data was analysed using SPSS software and results were interpreted into percentages. Doctors, nurses and lab technicians had good knowledge regarding biomedical waste management before but the knowledge of class IV employees (cleaners and maintenance personnel) was not good but all group showed good improvement in their Knowledge regarding biomedical waste management after training. There should be periodic trainings and workshops with special focus on proper use of personal protective measures for all health personnel having special focus on paramedical and cleaner staff about Biomedical waste management rules.
The level of blood lead painter workers Rafsanjan city and its correlation with risk factors for cardiovascular disease in 2019
Authors: Taheri Fard A , Ahmadinia H , Kafi M , Salandari M , Rahmani A , Rezaeian M 
Published: October 2020

Introduction: Painters are exposed to various chemical harmful agents such as lead particles in paints. Some studies have suggested a possible association of occupational exposure that may cause cardiovascular effects. Lead is absorbed through the body through the skin, skin, and respiratory tract and penetrates some tissues. Considering the importance of labor health in each country's development and progress, this study was conducted to determine the amount of lead in the blood of painters in Rafsanjan in 2019.
Materials and Methods: The present study is a cross-sectional study of the descriptive type conducted in 1397 on 77 painters of Rafsanjan city. Data collection was done in two stages, in the first stage, based on a checklist that included demographic information, and in the second stage by performing laboratory tests. Finally, by recording the data in SPSS software, quantitative data was reported as a "mean ± standard deviation" and qualitative information as a "number (percent)".
Results: Of the 77 painter workers, the youngest is 21, and the oldest is 70 years old. A total of 20 people (26%) had a family history of heart attacks. The highest amount of lead in the blood of painters was reported to be 30.7 micrograms per deciliter, and the level of lead was most correlated with blood sugar. Sixty people (77.9%) painters used personal protective equipment. 49.4% of the study's painters wore work clothes, and 28.6% used gloves while working. Lead levels are directly related to blood sugar, cholesterol, triglyceride, LDL, age, work experience, and blood sugar levels were most correlated with the level of lead in painters' blood.
Conclusion: This study's findings show that due to the increase in the work experience of painters, their blood lead level has also increased, and the blood sugar level has had the highest correlation with the lead level in the painters' blood.
Investigation of work ability index (WAI) and its relationship with maximal aerobic capacity (VO2max) among cement industry employer
Authors: Kavousian M , Salehi sashlabadi A , Jafari MJ , Khodakarim S , Rabiei H 
Published: October 2020

Introduction: Given the importance of adapting workers' physical and mental capabilities to their job needs, measuring their ability to work, maintaining, and upgrading, it has become an essential task. This study aimed to investigate WAI and its relationship with VO2max at one of the cement companies.
Materials and Methods: This cross-sectional study was conducted among 130 employees of a cement company in Iran in 2018. For data collection, the WAI, Queens's test for maximum oxygen consumption, and a questionnaire designed by researchers (socio-demographic and work-related factors) were used. SPSS 21 was used to analyze the data.
Results: The results showed that the mean ± standard deviation of WAI in staff was 39.35 ± 4.64. Among the demographic and related variables, sports activity (P > 0.04) and sleep quality (P < 0.001), and work experience (P> 0.046) were significantly correlated with WAI. There was a significant positive correlation between the mean score of WAI and Vo2max (r = 0.21, p < 0.05). Regression modeling showed that Vo2max was the only significant predictor of WAI.
Conclusion: According to the results of the study, to control and enhance the ability of the staff of the study, occupational intervention programs should focus on improving sleep quality and increased exercise. Also, considering the positive relationship of Vo2max to the WAI of the surveyed staff, it is recommended to select suitable employees in terms of aerobic capacity according to the workload of the job.
Risk assessment with intervention of gas pipelines repair using FMEA method and determination of safety performance monitoring: a case for a gas and oil operating company in 2012-13
Authors: Halvani GH , Mohammadnia MR , Zare Mehrjardi MR , Keshtvarz MR 
Published: October 2020

Introduction: work-related accidents impose irreparable hurt on the national capital. It is necessary to take a series of preventive measures before the occurrence to preserve and protect the human force and reduce accidents. The current study aimed to check safety contradiction using the FMEA method and determine protection indicators of safety performance in the overhaul project.
Materials and Methods: This study is descriptive-analytical research and an interference method that accomplished 13 jobs in major repairs gas pipeline project. After determining jobs and entering them in the FMEA worksheet, the initial risk score was obtained in this investigation, and after corrective suggestions, the secondary risk priority score was calculated.
Results: The relationship between RPN1 and RPN2 was examined and determined a significant difference between risk priority score before and after corrective measure, and this indicates that these measures are effective (p<0.0005). Among various jobs and before taking corrective actions, the highest number of accidents was related to excavation activities with 15 accidents, which were reduced to 6 after taking corrective measures. Most days lost from work were related to driving job with 6020 days that reduced to 8 days after taking corrective actions; the highest rate of repeat accidents with 62.5 was related to détente activity reduced to 23.14 after taking corrective measures. The maximum intensity factor with 19444.44 was related to the driving activity reduced to 25.25 after taking corrective actions. The accident frequency - severity rate in driving activity with 15.84 was the most reduced to 0.28 after taking corrective measures.
Conclusion: Due to unacceptable risks and the high rate of accidents in the overhaul of the gas pipeline project, appropriate control measures can be used to reach an acceptable level of risk, which represents the usefulness of the FMEA method.