bluuebunny/crossref_metadata_2025_split huggingface dataset:
How effective are digital interventions in increasing flu vaccination amongst pregnant women? A systematic review protocol
Authors: Parsons Joanne , Atherton Helen 
Published: May 2020

Abstract
Background
Pregnant women and their unborn babies are at increased risk of complications as a result of flu, yet uptake of the flu vaccination in the UK remains low. Digital interventions have proven effectiveness in changing health behaviour, but their effectiveness in increasing flu vaccination amongst pregnant women has not been examined. This protocol details the design and methodology of a systematic review and meta-analysis, examining the effectiveness of digital interventions in increasing flu vaccination amongst pregnant women.

Methods
Bibliographic databases will be searched using appropriate search terms related to vaccination, pregnancy and flu. Randomised, non-randomised, quasi randomised controlled trials and other quantitative study designs will be eligible for inclusion, and studies will present the rate of flu vaccination amongst pregnant women of digital interventions compared to non-digital interventions, or usual care. No date or study country restrictions will be put on included studies, but studies will be published in English.

Discussion
This is the first known systematic review to examine the effectiveness of digital interventions in increasing the rate of flu vaccination amongst pregnant women. This review has the potential to inform whether digital interventions are an appropriate and successful method of increasing flu vaccination amongst pregnant women, and to determine which mode of digital intervention is most effective.

Trial registration
This systematic review is registered on the international prospective register of systematic reviews (PROSPERO). Registration number pending.
Measure of noncompactness and a generalized Darbo’s fixed point theorem and its applications to a system of integral equations
Authors: Parvaneh Vahid , Khorshidi Maryam , De La Sen Manuel , Işık Hüseyin , Mursaleen Mohammad 
Published: May 2020

AbstractIşık et al. (Mathematics 7:862, 2019) presented an interesting generalization of the Banach contraction principle. In this paper, motivated by Işık et al., we give a new extension of the well-known Darbo inequality in a Banach space. Our results provide several generalizations of the Darbo inequality. As an application, we study the existence of solutions for a system of functional integral equations in$C[0,T]$C[0,T]. Finally, we expose a genuine example to support the effectiveness of our results.
On a fractional hybrid multi-term integro-differential inclusion with four-point sum and integral boundary conditions
Authors: Baleanu Dumitru , Etemad Sina , Rezapour Shahram 
Published: May 2020

AbstractWe investigate the existence of solutions for a fractional hybrid multi-term integro-differential inclusion with four-point sum and integral boundary value conditions. By using Dhage’s fixed point results, we prove our main existence result. Finally, we give an example to illustrate our main result.
The effect of a 5-year hand hygiene initiative based on the WHO multimodal hand hygiene improvement strategy: an interrupted time-series study
Authors: Suzuki Yumi , Morino Motoko , Morita Ichizo , Yamamoto Shigenori 
Published: May 2020

Abstract
Background
A World Health Organization (WHO) guideline-based multimodal hand hygiene (HH) initiative was introduced hospital-wide to a nonteaching Japanese hospital for 5 years. The objective of this study was to assess the effect of this initiative in terms of changes in alcohol-based hand rub (ABHR) consumption and the Hand Hygiene Self-Assessment Framework (HHSAF) score.

Methods
The consumption of monthly hospital-wide ABHR was calculated in L per 1000 patient days (PDs). The change in ABHR consumption was analysed by an interrupted time series analysis with a pre-implementation period of 36 months and an implementation period of 60 months. The correlation between annual ABHR consumption and the HHSAF score was estimated using Pearson’s correlation coefficients.

Results
The annual ABHR consumption was 4.0 (L/1000 PDs) to 4.4 in the pre-implementation period and 10.4 to 34.4 in the implementation period. The HHSAF score was 117.5 (out of 500) in the pre-implementation period and 267.5 to 445 in the implementation period. A statistically significant increase in the monthly ABHR consumption (change in slope: + 0.479 L/1000 PDs, p <  0.01) was observed with the implementation of the initiative. Annual ABHR consumption was strongly correlated with the annual HHSAF score (r = 0.971, p <  0.01).

Conclusions
A 5-year WHO-based HH initiative significantly increased ABHR consumption. Our study suggested that the HHSAF assessment can be a good process measure to improve HH in a single facility, as ABHR consumption increased with the HHSAF score.
Clinical characteristics and risk factors of polymicrobial Staphylococcus aureus bloodstream infections
Authors: Zheng Cheng , Zhang Shufang , Chen Qingqing , Zhong Li , Huang Tiancha , Zhang Xijiang , Zhang Kai , Zhou Hongwei , Cai Jiachang , Du Linlin , Wang Changming , Cui Wei , Zhang Gensheng 
Published: May 2020

Abstract
Background
Although Staphylococcus aureus bloodstream infections (SA-BSI) are a common and important infection, polymicrobial SA-BSI are infrequently reported. The aim of this study was to investigate the clinical characteristics and risk factors of polymicrobial SA-BSI in comparison with monomicrobial SA-BSI.

Methods
A single-center retrospective observational study was performed between Jan 1, 2013, and Dec 31, 2018 at a tertiary hospital. All patients with SA-BSI were enrolled, and their clinical data were gathered by reviewing electronic medical records.

Results
A total of 349 patients with SA-BSI were enrolled including 54 cases (15.5%) with polymicrobial SA-BSI. In multivariable analysis, burn injury (adjusted odds ratio [OR], 7.04; 95% confidence interval [CI], 1.71–28.94), need of blood transfusion (aOR, 2.72; 95% CI, 1.14–6.50), use of mechanical ventilation (aOR, 3.11; 95% CI, 1.16–8.30), the length of prior hospital stay (aOR, 1.02; 95% CI, 1.00–1.03), and pneumonia as primary site of infection (aOR, 4.22; 95% CI, 1.69–10.51) were independent factors of polymicrobial SA-BSI. In comparison with monomicrobial SA-BSI, patients with polymicrobial SA-BSI had longer length of ICU stay [median days, 23(6.25,49.25) vs. 0(0,12), p < 0.01] and hospital stay [median days, 50(21.75,85.75) vs. 28(15,49), p < 0.01], and showed a higher 28-day mortality (29.6% vs. 15.3%, p = 0.01).

Conclusions
Burn injury, blood transfusion, mechanical ventilation, the length of prior hospital stay, and pneumonia as a primary site of infection are independent risk factors for polymicrobial SA-BSI. In addition, patients with polymicrobial SA-BSI might have worse outcomes compared with monomicrobial SA-BSI.
Transplantation of bone marrow mesenchymal stem cells improves cognitive deficits and alleviates neuropathology in animal models of Alzheimer’s disease: a meta-analytic review on potential mechanisms
Authors: Qin Chuan , Lu Yalan , Wang Kewei , Bai Lin , Shi Guiying , Huang Yiying , Li Yongning 
Published: May 2020

AbstractBackgroundAlzheimer’s disease is a neurodegenerative disorder. Therapeutically, a transplantation of bone marrow mesenchymal stem cells (BMMSCs) can play a beneficial role in animal models of Alzheimer’s disease. However, the relevant mechanism remains to be fully elucidated.Main bodySubsequent to the transplantation of BMMSCs, memory loss and cognitive impairment were significantly improved in animal models with Alzheimer’s disease (AD). Potential mechanisms involved neurogenesis, apoptosis, angiogenesis, inflammation, immunomodulation, etc. The above mechanisms might play different roles at certain stages. It was revealed that the transplantation of BMMSCs could alter some gene levels. Moreover, the differential expression of representative genes was responsible for neuropathological phenotypes in Alzheimer’s disease, which could be used to construct gene-specific patterns.ConclusionsMultiple signal pathways involve therapeutic mechanisms by which the transplantation of BMMSCs improves cognitive and behavioral deficits in AD models. Gene expression profile can be utilized to establish statistical regression model for the evaluation of therapeutic effect. The transplantation of autologous BMMSCs maybe a prospective therapy for patients with Alzheimer’s disease.
Electrically stimulable indium tin oxide plate for long-term in vitro cardiomyocyte culture
Authors: Moon Sung-Hwan , Cho Young-Woo , Shim Hye-Eun , Choi Jae-Hak , Jung Chan-Hee , Hwang In-Tae , Kang Sun-Woong 
Published: May 2020

Abstract

Background
We investigated whether electrical stimulation via indium tin oxide (ITO) could enhance the in vitro culture of neonatal rat ventricular myocytes (NRVMs), which are important in vitro models for studying the mechanisms underlying many aspects of cardiology.

Methods
Cardiomyocytes were obtained from 1-day-old neonatal rat heart ventricles. To evaluate function of NRVMs cultured on ITO with electrical stimulation, the cell viability, change of cell morphology, immunochemistry using cardiac-specific antibodies, and gene expression were tested.

Results
Defined sarcomeric structure, cell enlargement, and increased distribution of NRVMs appeared in the presence of electrical stimulation. These characteristics were absent in NRVMs cultured under standard culture conditions. In addition, the expression levels of cardiomyocyte-specific and ion channel markers were higher in NRVMs seeded on ITO-coated dishes than in the control group at 14 days after seeding. ITO-coated dishes could effectively provide electrical cues to support the in vitro culture of NRVMs.

Conclusions
These results provide supporting evidence that electrical stimulation via ITO can be effectively used to maintain culture and enhance function of cardiomyocytes in vitro.
Construction of reporter gene assays using CWP and PDR mutant yeasts for enhanced detection of various sex steroids
Authors: Ito-Harashima Sayoko , Matano Mami , Onishi Kana , Nomura Tomofumi , Nakajima Saki , Ebata Shingo , Shiizaki Kazuhiro , Kawanishi Masanobu , Yagi Takashi 
Published: May 2020

Abstract
Background
Sex steroid hormone receptors are classified into three classes of receptors: estrogen receptors (ER) α and β, androgen receptor (AR), and progesterone receptor (PR). They belong to the nuclear receptor superfamily and activate their downstream genes in a ligand-dependent manner. Since sex steroid hormones are involved in a wide variety of physiological processes and cancer development, synthetic chemical substances that exhibit sex steroid hormone activities have been applied as pharmaceuticals and consumed in large amounts worldwide. They are potentially hazardous contaminants as endocrine disruptors in the environment because they may induce inappropriate gene expression mediated by sex steroid hormone receptors in vivo.

Results
To develop simple reporter gene assays with enhanced sensitivity for the detection of sex steroid hormones, we newly established mutant yeast strains lacking the CWP and PDR genes encoding cell wall mannoproteins and plasma membrane drug efflux pumps, respectively, and expressing human ERα, ERβ, AR, and PR. Reporter gene assays with mutant yeast strains responded to endogenous and synthetic ligands more strongly than those with wild-type strains. Sex steroid hormone activities in some pharmaceutical oral tablets and human urine were also detectable in these yeast assays.

Conclusions
Yeast reporter gene assay systems for all six steroid hormone receptors, including previously established glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) assay yeasts, are now available. Environmental endocrine disrupters with steroid hormone activity will be qualitatively detectable by simple and easy procedures. The yeast-based reporter gene assay will be valuable as a primary screening tool to detect and evaluate steroid hormone activities in various test samples. Our assay system will strongly support the detection of agonists, antagonists, and inverse agonists of steroid hormone receptors in the field of novel drug discovery and assessments of environmental pollutants.
Mitigation of adverse effects of salinity stress on sunflower plant (Helianthus annuus L.) by exogenous application of chitosan
Authors: Bakhoum Gehan Shaker , Sadak Mervat Shamoon , Badr Elham Abd El Moneim 
Published: May 2020

Abstract
Background
Most developing countries are suffering from a decline in agricultural area and fresh water supply due to the adverse effects of climate change. Sunflower has been considered as having the ability to grow under various environmental conditions, tolerating levels considered stresses to other plants.

Materials and Methods
Thus, two field experiments were conducted in the screen of National Research Centre during two successive summer seasons 2018 and 2019, respectively to investigate the effect of soaking seeds of sunflower plant in different concentrations of chitosan (control, 25, 50 and 75 mg/L) on growth, some physiological parameters, yield and its components and some chemical composition of the yielded seeds of sunflower under different salinity levels (control, 4000 and 8000 mg/l).

Results
The obtained results showed that salt stress with 4000 mg/L recorded the highest values of all the studied growth characters, photosynthetic pigments, some physiological aspects, seed yield/plant and oil %. On the other hand, the highest values of head diameter, head circumference, weight 50 seed and crude protein were obtained by control treatment. Meanwhile, the highest values of proline and free amino acid were recorded under higher salinity level (8000 mg/L). On the other hand, all chitosan treatments significantly enhanced growth and productivity of sunflower plant. Also, the obtained data that, chitosan concentration (50 mg/L) recorded the highest values of all the studied growth and yield quantity and quality via enhancing photosynthetic pigments and the studied physiological aspects, as compared with other treatments. As for interaction effect, plants treated with chitosan concentration (50 mg/L) under salinity level (4000 mg/L) recorded the highest growth characters, photosynthetic pigments and IAA. While the interaction between chitosan concentrations (50 mg/L) under salinity level (8000 mg/L) recorded the highest yield characters, except for crude protein (75 mg/L chitosan) in control plants (those plants irrigated with tap water).

Conclusion
In conclusion, chitosan treatments did not only improve plant growth and productivity but also could enhance the reducing effects of salinity stress on growth and productivity of sunflower plant. The most effective concentrations was 50 mg/L on increasing growth and yield of sunflower plant.
Reducing work pressure and IT problems and facilitating IT integration and audit & feedback help adherence to perioperative safety guidelines: a survey among 95 perioperative professionals
Authors: Emond Yvette E. J. J. M. , Wolff André P. , Peters Yvonne A. S. , Bloo Gerrit J. A. , Westert Gert P. , Damen Johan , Calsbeek Hiske , Wollersheim Hub C. 
Published: May 2020

Abstract
Background
To improve perioperative patient safety, guidelines for the preoperative, peroperative, and postoperative phase were introduced in the Netherlands between 2010 and 2013. To help the implementation of these guidelines, we aimed to get a better understanding of the barriers and drivers of perioperative guideline adherence and to explore what can be learned for future implementation projects in complex organizations.

Methods
We developed a questionnaire survey based on the theoretical framework of Van Sluisveld et al. for classifying barriers and facilitators. The questionnaire contained 57 statements derived from (a) an instrument for measuring determinants of innovations by the Dutch Organization for Applied Scientific Research, (b) interviews with quality and safety policy officers and perioperative professionals, and (c) a publication of Cabana et al. The target group consisted of 232 perioperative professionals in nine hospitals. In addition to rating the statements on a five-point Likert scale (which were classified into the seven categories of the framework: factors relating to the intervention, society, implementation, organization, professional, patients, and social factors), respondents were invited to rank their three most important barriers in a separate, extra open-ended question.

Results
Ninety-five professionals (41%) completed the questionnaire. Fifteen statements (26%) were considered to be barriers, relating to social factors (N = 5), the organization (N = 4), the professional (N = 4), the patient (N = 1), and the intervention (N = 1). An integrated information system was considered an important facilitator (70.4%) as well as audit and feedback (41.8%). The Barriers Top-3 question resulted in 75 different barriers in nearly all categories. The most frequently reported barriers were as follows: time pressure (16% of the total number of barriers), emergency patients (8%), inefficient IT structure (4%), and workload (3%).

Conclusions
We identified a wide range of barriers that are believed to hinder the use of the perioperative safety guidelines, while an integrated information system and local data collection and feedback will also be necessary to engage perioperative teams. These barriers need to be locally prioritized and addressed by tailored implementation strategies. These results may also be of relevance for guideline implementation in general in complex organizations.

Trial registration
Dutch Trial Registry: NTR3568.
Targeted Next-Generation Sequencing for Congenital Hypothyroidism With Positive Neonatal TSH Screening
Authors: Yamaguchi Takeshi , Nakamura Akie , Nakayama Kanako , Hishimura Nozomi , Morikawa Shuntaro , Ishizu Katsura , Tajima Toshihiro 
Published: May 2020

Abstract

Purpose
Congenital hypothyroidism (CH) is the most common neonatal endocrine disorder; however, its molecular etiology remains poorly understood.

Methods
We performed genetic analysis of 24 causative genes using next-generation sequencing in 167 CH cases, comprising 57 dyshormonogenesis (DH), 32 dysgenesis (TD) and 78 undiagnosed. The pathogenicity of variants was assessed by the American College of Medical Genetics guidelines, inheritance pattern, and published evidence. Furthermore, we compared the oligogenic groups and monogenic groups to examine the correlation between variant dosage and severity.

Results
We identified variants in 66.5% cases (111/167) and 15 genes, DUOX2, TSHR, PAX8, TG, TPO, DUOXA2, JAG1, GLIS3, DUOX1, IYD, SLC26A4, SLC5A5, SECISBP2, DIO1, and DIO3. Biallelic variants were identified in 12.6% (21/167), oligogenic in 18.0% (30/167), and monogenic in 35.9% (60/167); however, 68.5% of variants were classified as variant of unknown significance (VUS). Further examinations showed that 3 out of 32 cases with TD (9.4%) had pathogenic variants (2 of TSHR and 1 of TPO), and 8 out of 57 cases with DH (14.0%) (7 of DUOX2, 1 of TG) had pathogenic variants. In addition, TSH levels at the first visit were significantly higher in the oligogenic group than in the monogenic group.

Conclusions
The detection rate of pathogenic variants in Japanese CH was similar to that previously reported. Moreover, oligogenic cases were likely to be more severe than monogenic cases, suggesting that CH may exhibit a gene dosage effect. Further analysis of VUS pathogenicity is required to clarify the molecular basis of CH.
Quantitative single-cell interactomes in normal and virus-infected mouse lungs
Authors: Cain Margo P. , Hernandez Belinda J. , Chen Jichao 
Published: May 2020

ABSTRACT
Mammalian organs consist of diverse, intermixed cell types that signal to each other via ligand-receptor interactions – an interactome – to ensure development, homeostasis and injury-repair. Dissecting such intercellular interactions is facilitated by rapidly growing single-cell RNA sequencing (scRNA-seq) data; however, existing computational methods are often not readily adaptable by bench scientists without advanced programming skills. Here, we describe a quantitative intuitive algorithm, coupled with an optimized experimental protocol, to construct and compare interactomes in control and Sendai virus-infected mouse lungs. A minimum of 90 cells per cell type compensates for the known gene dropout issue in scRNA-seq and achieves comparable sensitivity to bulk RNA sequencing. Cell lineage normalization after cell sorting allows cost-efficient representation of cell types of interest. A numeric representation of ligand-receptor interactions identifies, as outliers, known and potentially new interactions as well as changes upon viral infection. Our experimental and computational approaches can be generalized to other organs and human samples.
The Lumiptosome, an engineered luminescent form of the apoptosome can report cell death by using the same Apaf-1 dependent pathway
Authors: Hosseini Elaheh Sadat , Nikkhah Maryam , Hamidieh Amir Ali , Fearnhead Howard O. , Concordet Jean-Paul , Hosseinkhani Saman 
Published: May 2020

ABSTRACT
Detection of the apoptosis signature becomes central in understanding cell death modes. We present here a whole-cell biosensor that detects Apaf-1 association and apoptosome formation using a split-luciferase complementary assay. Fusion of N-terminal (Nluc) and C-terminal (Cluc)-fragments of firefly luciferase to the N-terminus of human Apaf-1 was performed in HEK293 cells by using CRISPR-Cas9 technology. This resulted in a luminescent form of the apoptosome that we named ‘Lumiptosome’. During Apaf-1 gene editing, a high number of knock-in events were observed without selection, suggesting that the Apaf-1 locus is important for the integration of exogenous transgenes. Since activation of caspase-9 is directly dependent on the apoptosome formation, measured reconstitution of luciferase activity should result from the cooperative association of Nluc-Apaf-1 and Cluc-Apaf-1. Time-response measurements also confirmed that formation of the apoptosome occurs prior to activation of caspase-3. Additionally, overexpression of the Bcl2 apoptosis regulator in transgenic and normal HEK293 cells confirmed that formation of the Lumiptosome depends on release of cytochrome c. Thus, HEK293 cells that stably express the Lumiptosome can be utilized to screen pro- and anti-apoptotic drugs, and to examine Apaf-1-dependent cellular pathways.