bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nyanlända gymnasieelever – Elevidentiteter och språkbarriärer
Authors: Aho Erika Bomström 
Published: March 2020

I föreliggande artikel studeras nyanlända elevers upplevelser av att vara elever på Språkintroduktion. Programmet är ett av fem introduktionsprogram i gymnasieskolan och tar emot nyanlända elever från 16 till 19 år. Eleverna placeras på Språkintroduktion eftersom de nyligen har kommit till Sverige och för att de ännu inte uppnått tillräckliga kunskaper i det svenska språket. I denna intervjustudie bidrar Homi Bhabhas teori om mellanrummet till en förståelse för hur erfarenheter av Språkintroduktion kan upplevas. Resultatet visar att elevgrupperna på Språkintroduktion är heterogena eftersom eleverna har olika erfarenheter av skola och arbete. Tre teman blir synliga i resultatet: elever med andra kunskaper och erfarenheter, elevidentiteter som särskilt framträder och det svenska språket som barriär. Resultaten visar att förvärvandet av det svenska språket för varje elev blir ett mål att nå samtidigt som det utgör ett hinder. Språket blir viktigt eftersom eleverna är beroende av det för att lyckas såväl i skolan som i samhällslivet. Likaså blir synliggörandet av elevers tidigare erfarenheter och kunskaper betydelsefullt eftersom det kan komma att påverka deras identitetsuppfattning.
Nyckelord: språkintroduktion; nyanlända elever; mellanrum; elevidentitet
Newly arrived upper secondary school students – Student identities and language barriers
AbsractThis study investigates newly arrived students’ experiences of being students at Language Introduction in Sweden. The program is one of five introductory programs in Swedish upper secondary school and admits newly arrived students from the ages of 16 to 19 years old. Students are placed at Language introduction because they have recently arrived in Sweden and since they have not yet gained enough knowledge of the Swedish language. In this interview study, Homi Bhabha’s theory of the third space contributes to an understanding of how experiences from Language introduction can be perceived. The results show that student groups at Language introduction are heterogeneous as students have diverse experiences of school and work. Three themes appear in the results: students with other kinds of knowledge and experiences, student identities in transformation, the Swedish language as an obstacle and as goal. The results show that the acquisition of the Swedish language becomes to each student a goal to reach and at the same time a hindrance. The language becomes important because the students depend on it in order to be successful in school as well as in life in society. Likewise, making the students’ past experiences and knowledge visible, becomes important as it may affect their perception of identity.
Keywords: språkintroduktion; nyanlända elever; mellanrum; elevidentitet
Revisão bibliográfica sobre as variáveis associadas aos componentes do tráfego que influenciam a segurança viária nos entornos escolares
Authors: Oestreich Letícia , Torres Tânia Batistela , Pereira Brenda Medeiros , Padillo Alejandro Ruiz - 
Published: March 2020

Traffic accidents are the main cause of death for children and young people all over the world, so it is necessary to explore road safety measures to ensure the right to life of this population group. Understanding the measures to improve road safety conditions in school environments contributes to the safety of children and young people in school. The objective of this study is to define variables associated with traffic components that affect road safety conditions in school environments through an exploratory bibliographic research. As a result, the study provides a summary of the main variables found in the literature related to the subject and discusses the performance of these variables on traffic components and the impact road safety in school environments. Results of this study provide support for measures to improve road safety in school environments and encourage the use of more sustainable modes of transport, as well as, support the elaboration of research related to the theme.
Desenvolvimento de equipamento de autoteste para defeitos em placas de potência de lavadoras de roupas
Authors: Silva Ronaldo Barcelos e , Quadros Glauber Rodrigues de , Fabris Lucinéia , Cardoso Lucas Tavares , Boita Jocenir , Maldaner Silvana 
Published: March 2020

In this work a test equipment called RBS was developed to monitor the operation of a washing machine power board. The RBS identify the main defects of Brastemp and Consul washing machines. The developed RBS equipment verifies that the machine's power plate is sending electrical signal to its peripherals such as valve 1, the drainage pump, the clockwise motor and the counterclockwise motor. The operation of each machine peripherals is represented by an LED on the circuit. The defect in the board can be verified by the LED that does not light up in the circuit. Thus, through RBS it is possible to save time in identifying washing machine problems and to obtain a reliable test system after repairing them. Additionally, it is important to note that the cost of the equipment developed is approximately R$ 26.00, while commercial self-test systems cost approximately two hundred reais.
Vo2 máximo obtido com e sem teste ergométrico
Authors: Ribeiro Cléo Pereira , Daronco Luciane Sanchotene Etchepare , Jacobi Luciane Flores , Silva Luciano Panosso da 
Published: March 2020

Objetivo: verificar a concordância e a correlação entre duas metodologias distintas, utilizadas na obtenção do VO2 máx, as propostas por Foster et al. (T1) e Jackson et al., modelo 2, (T2). Metodologia: Os dados para análise, pertencentes a uma clínica privada em nosso município, foram obtidos mediante a assinatura dos Termos de Confidencialidade e Autorização Institucional. De acordo com os critérios de inclusão, 106 sujeitos (29 homens; 77 mulheres) formaram a amostra. O teste de Shapiro-Wilk, a análise descritiva, a análise de Bland e Altman e o coeficiente de correlação de Pearson, compuseram a análise estatística (nível de significância de 5%). Resultados: apesar das semelhanças, entre as médias obtidas, as metodologias demonstraram concordância somente no grupo masculino. Conclusão: em função dos resultados obtidos, e da enorme praticidade, sugere-se o método T2 para fins de prescrição do exercício, mas, o método T1 torna-se indispensável para avaliar a integridade do sistema cardiorrespiratório.
Bystander effect formation in the sprout root meristem in condition of irradiated and non-irradiated seeds joint growing
Authors: German O. Yu. 
Published: March 2020

Aim. To study the possibility of bystander effect inducing in the joint germination in a common aqueous environment of irradiated and non-irradiated seeds. Methods. Cytogenetic methods including mitotic activity level estimation, accounting for the number of nucleoli, and methods for statistical analysis of the data. Results. The effect of radiation stimulation with a dose of 40 Gy was dependent on the variety, and the effect of gamma radiation at a dose of 200 Gy reduced the level of mitotic activity in comparison with the control in both varieties. Mitotic indices increase in the meristem of non-irradiated seeds while growing with irradiated one. Conclusions. The bystander effect is formed on the organismic level under condition of growing of irradiated and non-irradiated seeds in a common aquatic environment and it is ma-nifested as an increase in the level of meristem cells proliferation and in the intensification of synthetic processes. The addition of irradiated with a high dose of gamma radiation seeds to the non-irradiated seeds induces an increase in mitotic activity largely than the addition of seeds irradiated with a relatively small dose.
Keywords: bystander effect, radiation stimulation, mitotic activity, nucleoli.
PTEN-dependent changes of gene expressions in human prostate tumors
Authors: Gerashchenko G. V. , Grygoruk O. V. , Mevs L. V. , Kashuba V. I. 
Published: March 2020

Aim. To determine the levels of PTEN relative expression (RE) and the possible effects of its loss on some tumor-associated genes and histological characteristics of human prostate tumors. Methods. PTEN RE and 46 genes were identified in 37 pairs of adenocarcinoma and conventionally normal tissues (CNT) and 20 adenomas by real time PCR. Results. We are shown PTEN RE decreasing in the adenocarcinoma group as well as in CNTs compared with adenomas. 54 % of adenocarcinomas, 48.6 % of CNT and 25 % of adenomas were detected with low PTEN RE. We have found 9 of 46 differentially expressed genes in groups with different levels of PTEN RE (ESR1, GCR, KRT18, MMP2, MMP9, SRD5A2, VIM, PCA3, HOTAIR). It was revealed some histological characteristics correlated with PTEN RE in prostate adenocarcinomas. Conclusions. PTEN aberrations in prostate tumors indicate that PTEN associated changes already have been occurred at the benign tumors. We have shown some tumor histological characteristics associated with PTEN expression changes.Keywords: prostate tumors, relative gene expression, PTEN, EMT, histological characteristics.
Optimization of ChIP protocol for investigation of canonical Wnt target genes expression in new born heart
Authors: Piven O. O. 
Published: March 2020

Aim. In present work we have focused on chromatinimmunoprecipitation (ChIP) protocol with next qPCR optimization for analysis of canonical Wnt target genes regulation by b‑catenin and plakoglobin in new born heart. Methods. In study we have used the new born hearts tissue at the age of 3 days after birth (P3). We have analyzed hearts of control mice and mice with heterozygous knockout of β-catenin. We have proceeded ChIP protocol based on standard protocols in our modifications. ChIP results validated with qPCR. Results. We have adopted ChIP protocol for investigation of signalling function of β-catenin and plakoglobin as well as for identification of β-catenin and plakoglobin specific binding genomic region in new born heart. Conclusions. We have shown that plakoglobin is able to compensate the signaling function of β-catenin and binds with similar genomic region under β-catenin haploinsufficiency conditions.
Keywords: β-catenin, plakoglobin, Wnt signaling, gene expression, heart, chromatinimmunoprecipitation, ChIP.
Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene
Authors: Honcharuk O. M. , Dubrovna O. V. 
Published: March 2020

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes.
Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.
The combination of NO donor and ferulic acid effect on the elicitation of Triticum aestivum tolerance against Septoria tritici
Authors: Zhuk I. V. , Dmitriev A. P. , Lisova G. M. , Kucherova L. O. 
Published: March 2020

Aim. The aim is to research the ability of enhancing ferulic acid effect as a biotic elicitor to induce tolerance of winter wheat plants against Septoria tritici Rob et Desm. leaf blotch infection by addition donor of NO signal molecule. Methods. Content of endogenous H2O2 was measured in wheat leaves (cv. Oberig myronivskij and Svytanok myronivskij). The extent of disease development, morphometric parameters and yield structure were analyzed. Results. It is shown that combination of treatment by ferulic acid with NO donor reduced the disease symptoms on 1–2 points. The level of endogenous hydrogen peroxide increased on 27 % in cv. Svytanok myronivskij. Conclusions. The data obtained suggest that combination of ferulic acid with donor NO could be used as more effective combination than biotic elicitor. They decreased the degree of lesions in leaf area caused by Septoria tritici Rob et Desm. leaf blotch infection and stimulated the growth of wheat plants. The analyze of biochemical mechanisms revealed that system resistance of wheat plants is induced via activation of antioxidant protection. Keywords: ferulic acid, NO, biotic elicitors, induced resistance, Triticum aestivum L., Septoria tritici Rob et Desm.
Genotypic features of morphogenetic reactions of varieties and hybrids F1 of winter soft wheat during various stages of androgenesis in vitro
Authors: Zambriborshch I. S. , Shestopal O. L. , Boyko M. S. 
Published: March 2020

Aim. Testing the haploproduction ability of 47 varieties and 143 F1 hybrids of winter bread wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of сallus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The range of variation haploproduction activiti was broad. The sign of "the formation of сallus" for varieties was in limited from 0 to 21.2 % and for hybrids – from 0 to 38.8 %; on the sign of "regeneration of green plants" for varieties – from 0 to 9.4 %, and for hybrids from 0 to 9.1 %. The positive effect of 1BL/1RS translocation on signs of haploproduction in anther culture Triticum aestivum L. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. It has been found that F1 hybrids have a higher potential production potential in anther culture than varieties. During the period from 2011 to 2017, the 580 lines of winter wheat were transferred to the breeding departments.
Keywords: soft winter wheat, anther culture in vitro, callus, regeneration.
Applying the nanobiotechnological methods for optimize the in vitro cultivation technology for cows oocytes
Authors: Kovtun S. I. , Zyuzyun A. B. , Shcherbak O. V. , Trotskiy P. A. 
Published: March 2020

Aim. Investigated effect nanomaterial of highly dispersed ultra fine silica (UFS) by carbohydrate – sucrose (UFS/sucrose) on the effectiveness of meiotic maturation cows oocytes in vitro. Methods. The fresh and frozen – thawed cow oocyte-cumulus complex (OCC) was divided into four groups: three experimental in which the cultivation was carried out in a medium containing of 0, 1; 0, 01 and 0,001 % UFS/sucrose and control without adding nanomaterial. Results. It was concluded that the addition of UFS/sucrose in 0.001 % concentration is effective for elevation level of oocytes maturation and provides reception of 76,8% oocytes that induced the metaphase II of meiosis. Adding carbohydrate – sucrose (UFS/sucrose) in 0.001 % concentration to the culture medium frozen – thawed cow generative cells, make positive effect on in vitro fertilization and provide embryos quantity enhancement to 33.3 %. Conclusions. Addition of UFS/sucrose in 0.001 % concentration to the culture medium have increase effect and promote level in vitro maturation of cows oocytes rising to 76.8 %. Usage of UFS/sucrose in 0.001 % concentration as part of in vitro culture medium for cows oocyte-cumulus complex conduce rising quantity of cattle embryos to 33.3 % after in vitro fecundation frozen – thawed and maturation oocytes.
Keywords: oocytes, in vitro culture, embryos, nanomaterial, ultra fine silica (UFS).
DNA certification of varieties and hybrids of perennial grass crops
Authors: Kondratskaya I. P. , Yukhimuk A. N. , Chizhik O. V. , Reshetnikov V. N. , Stolepchenko V. A. , Vasko P. P. 
Published: March 2020

Aim. The aim of this study was to carry out passportization of cultivars, varieties and hybrids of perennial grass crops: Alopecurus protensis, Agropyron cristatum, Lolium perenne, Lolium miltiflorum, Festuca protensis, Festuca arundinasea, hybrids Festulolium. Methods. The perennial grasses selection was carried out by embryo culture according to methodical recommendations. For the molecular-genetic passportization of perennial grasses varieties the polymorphous primers have been selected – 9 RAPD primers and 15 ISSR primers. Results. Multilocus DNA labeling have allowed to differentiate all the genotypes being studied, to develop and to create the unique profiles for each of them and to calculate the genetic distance of congeniality/distances. On the base of RAPD/ISSR, SCoT spectra the 14 molecular-genetic passports of perennial grass cops have been obtained for the first time.
Keywords: grass crops, molecular-genetic passport, DNA, PCR, multipoint mapping RAPD/ISSR spectra.
A high-throughput virtual screening in Grid for new tubulin-targeted inhibitors of plant fungal pathogens
Authors: Karpov P. A. , Demchuk O. M. , Rayevsky O. V. , Ozheredov S. P. , Spivak S. I. , Samofalova D. O. , Blume Ya. B. 
Published: March 2020

Aim. To select new tubulin-targeted inhibitors of plant fungal pathogens based on results of high-throughput virtual screening in Grid. Methods. Protein and ligand spatial structure modelling (I-Tasser, Grid), design and virtual screening ligands library (UCSF Dock 6, Grid), molecular docking (CCDC Gold), molecular dynamics simulation (Gromacs, Grid). Results. 240 structural models of tubulin molecules (82 α-, 111 β- and 47 γ-tubulin) from 62 species of phytopathogenic fungi were constructed. It was found that imidazole ligands, demonstrate strongest affinity to α- and β-tubulin. It was found that among α-, β- and γ-tubulin, taxol binding site of β-tubulin possess the strongest potential as the fungicidal drugs target. It was selected 50 leader compounds: 23 with affinity for GTP/GDF-exchange site and 27 with affinity for taxol-binding site. Conclusions. It was found, that in phytopathogenic fungi, taxol binding site of β-tubulin are the main fungicid drug target (in compare to other tubulin site or isotype). The highest affinity was predicted for the compounds F0478-0219, F0478-0166 and β-tubulin from Puccinia graminis f. sp. Tritici, as well as for the compound F0478-0385 and β-tubulin from Magnaporthe oryzae.
Keywords: pathogenic fungi, fungicides, tubulin, virtual screening, Grid.
The course of anthropogenetics in a classical university
Authors: Atramentova L. A. 
Published: March 2020

The article describes the principles of teaching the course of anthropogenetics in a classical university, taking into account the needs of various branches of science and practical activity: medicine, pedagogy, psychology, criminology, pharmacology, etc. The main examples of the most frequent mistakes in the planning and organization of anthropogenetic research are given. These examples concern the approaches and methods of genetics in solving scientific and practical problems associated with human heredity. The most outstanding achievements of Kharkov anthropogenetic school as the center of systematic research in the field of human genetics in Ukraine are described. The description of the training course of anthropogenetics, taught at the Biological Faculty of the V.N. Karazin Kharkov National University is given.
Keywords: anthropogenetics.
Methodological problems of biotic diagnostics of antropogenic transformation of forest ecosystems at the levels of organization of life
Authors: Lavrov V. V. , Blinkova O. I. 
Published: March 2020

Aim. The purpose of the study was to identify and systematize the methodological problems of the diagnosis of anthropogenic transformation of forests by the levels of organization of life. Methods. Theoretical methods of system-structural, retrospective, comparative analysis were combined with special methods of forestry, synecology, mycology, ornithology. Results and conclusions. The study of higher levels of biological organization, integral characteristics of ecosystems, reflecting the state of their integrity and development should be given priority in bio-diagnostics of forest ecosystems. Studies at lower levels of the hierarchy of levels without proper alignment with the composition and state of higher subsystems can lead to false conclusions. Studying at the basic levels of biological organization gives the opportunity to adhere to the principles of emergence, functional integration and hierarchical organization of the biosphere and its homeostasis systems.
Keywords: biological organization, complex of biological structures, forest ecosystem, transformation.
Do not forget the name of V.P. Efroimson (1908–1989)
Authors: Etkalo E. N. 
Published: March 2020

In 2018, 110 years have passed since the birth of Vladimir Pavlovich Efroimson (1908–1989). Efroimson studied at the biological department of Moscow University, in 1929 was expelled for the support of S.S. Chetveryakov. In December 1932 he was arrested. Since 1935 works in the Central Asian Institute of Sericulture, but in August 1937 he was dismissed from the Institute for his performance in defense of "Mendelism-Morganism". In the same year he was declared an enemy of the people, and he leaves for Ukraine. During this period he wrote a book on "Genetics and breeding of the silkworm". In May 1941, Efroimson defended his Ph.D. thesis at Kharkov University and went to the front. Rewarded by the Red Star and Patriotic War II degree. After the war, Efroimson returned to Kharkov University as an assistant professor of the Department of Darwinism and Genetics. In 1947 he defended his doctoral dissertation on the problems of evolutionary genetics and breeding of the silkworm. In 1951 he was sentenced to 10 years in prison. After rehabilitation he returns to Moscow and works as a bibliographer. In 1967, he received the title of professor and worked in the Institute of Psychiatry. He published a number of monographs, among them "Genetics of Oligophrenia, Psychoses and Epilepsy". His well-known books on medical genetics, anthropogenetics, gene determination of intelligence and behavior: "Genius and Genetics", "Genetics of Ethics and Aesthetics", "Pedagogical Genetics".
Keywords: genetics, history of science, V.P. Efroimson.