bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gaining favorable identities of Iranian EFL teachers: A single case study of transition from elementary schools to universities
Authors: Rostami Farzad , Yousefi Mohammad Hossein - , Amini Davood 
Published: February 2020

Abstract
Having adapted the self-concept framework as a lens and intrinsic case study as a methodological tool, the present study set out to examine how the professional identity of an elementary school teacher is socially (re) constructed while his transition from teaching at elementary schools to teaching at universities. The design of the study was the intrinsic case study. The focal participant was an assistant professor of TEFL who has been teaching in elementary, middle and high schools prior to starting his academic work ten years ago. For the purpose of the data collection, in-depth interview was conducted. Another source of the data was the participant's stories as well as the diaries he had kept over the years. For the data analysis, thematic analysis was employed. Results indicate that the transition was seen to be in parallel with the transition from lower identity perception to higher identity perception. The transitions can be best expressed in terms of identity promotion. The results indicate that the transitions were two main and noticeable ones. The data analyses also indicate that three themes including personal-self, social self, and learners were the driving forces for the case's professional identity development. The study has a number of implications for teacher education, policymakers, and teachers.
Exposure to desflurane anesthesia confers colorectal cancer cells metastatic capacity through deregulation of miR-34a/LOXL3
Authors: Ren Junyi , Wang Xiaopeng , Wei Gang , Meng Yajing 
Published: February 2020

Abstract
Background: Due to high potency and low toxicity, desflurane has been wildly used during surgery. Recent evidence that the use of desflurane was associated with colorectal cancer (CRC) tumor metastasis and poor prognosis raising concerns about the safety of desflurane. However, the mechanism was uncovered.Methods: CRC cells were exposed to desflurane, the changes in morphology and epithelial-mesenchymal transition (EMT)-related genes were evaluated. Transwell assay was used to study the migration and invasion effect. Xenograft was performed to study the tumor formation ability of desflurane-treated cells in vivo. Dual luciferase reporter assay was conducted to verify the target of miR-34a. Knockdown or overexpression of LOXL3 was used to investigate the mechanism of desflurane-induced EMT. The association of LOXL3 with CRC molecular subtypes and clinical relevance was studied by analysis of public datasets. Results: Exposure to desflurane induced EMT, migration, and invasion in CRC cells. Mice injected with desflurane-treated cells formed more tumors in the lungs. Downregulation of miR-34a and upregulation of LOXL3 were required for desflurane-induced EMT in CRC cells. LOXL3 was a direct target of miR-34a. Overexpression of LOXL3 rescued miR-34a-repressed EMT after exposure to desflurane. Elevated expression of LOXL3 was enriched in CMS4 and CRIS-B subtypes. Patients with high expression of LOXL3 showed more lymph node metastasis, as well as poor survival.Conclusion: Desflurane induced EMT and metastasis in CRC through deregulation of miR-34a/LOXL3 axis. Clinical miR-34a mimic or inhibitor targeting LOXL3 might have a potential protective role when CRC patients anesthetized by desflurane.
TRIM3 inhibits P53 signaling in breast cancer cells
Authors: Wang Xinxing , Zhang Yujie , Pei Xinhong , Guo Guangcheng , Xue Bingjian , Duan Xin , Dou Dongwei 
Published: February 2020

Abstract
BackgroundBeast cancer is the most common women cancer worldwide, while two third of them are ER alpha positive breast cancer. Among the ER alpha positive breast cancer, about 80% are P53 wild type, indicating the potential tumor suppression role in ER alpha positive breast cancer. Since P53 is an important safeguard to inhibit cell malignant transformation, reactivating P53 signaling could a plausible approach to treat breast cancer.MethodsTRIM3 protein levels were measured by western blot, while the P53 classical target genes were measured by real-time PCR. WST1 assay were used to measure cell proliferation, while cleaved caspase-3 was used to detect cell apoptosis. Protein stability and ubiquitin assay were used to detect the P53 protein ubiquitin and stability. The immuno-precipitation assays were used to detect the protein interactions. Immuno-staining was used to detect the protein localization of P53 and TRIM3, while the ubiquitin-based immuno-precipitation assays were used to detect the specific ubiquitination manner of P53.ResultsIn our study, we identified TRIM3 as an endogenous inhibitor for P53 signaling. TRIM3 depletion inhibited breast cancer cell proliferation and promoted apoptosis. In addition, TRIM3 depletion increased P53 protein level in breast cancer cell. Further investigation showed that TRIM3 could associate with P53 and promote P53 K48-linked ubiquitination and degradation.ConclusionOur study identified a novel post-translational modification mechanism between TRIM3 and P53. TRIM3 depletion or blockage could be a promising strategy to rescue P53 signaling and inhibit breast cancer progression.Highlights1. TRIM3 facilitates breast cancer cell growth and anti-apotosis.2. TRIM3 inhibits P53 protein and its signaling activity.3. TRIM3 interacts with P53 and promotes P53 K48-linked ubiquitination and degradation.
Global Health in Preconception, Pregnancy and Postpartum Alliance: Development of an International Consumer and Community Involvement (CCI) Framework.
Authors: Bergmeier Heidi , Vandall-Walker Virginia , Skrybant Magdalena , Teede Helena J , Bailey Cate , Baxter Jo-Anna B , Borges Ana Luiza Vilela , Boyle Jacqueline A , Everitt Ayesha , Harrison Cheryce L , Herrera Margely , Hill Briony , Jack Brian , Jones Samuel , Jorgensen Laura , Lim Siew , Montanaro Cynthia , Redman Leanne , Stephenson Judith , Sundseth Hildrun , Thangaratinam Shakila , Thynne Paula , Walker Ruth , Skouteris Helen 
Published: February 2020

Abstract
Background: The goal of the Global Health in Preconception, Pregnancy and Postpartum (HiPPP) Alliance, comprising consumers and leading international multidisciplinary academics and clinicians, is to generate research and translation priorities and build international collaboration around healthy lifestyle and obesity prevention among women across the reproductive years. In doing so, we actively seek to involve consumers in research, implementation and translation initiatives. There are limited frameworks specifically designed to involve women across the key obesity prevention windows before (preconception), during and after pregnancy (postpartum). The aim of this paper is to outline our strategy for the development of the HiPPP CCI Framework, with consumers as central to co-designed, co-implemented and co-disseminated research and translation. Method: The development of the framework involved three phases: In Phase 1, 21 Global HiPPP Alliance members participated in a CCI workshop to propose and discuss values and approaches for framework development; Phase 2 comprised a search of peer-reviewed and grey literature for existing CCI frameworks and resources; and Phase 3 entailed collaboration with consumers (i.e., members of the public with lived experience of weight/lifestyle issues in preconception, pregnancy and postpartum) and international CCI experts to workshop and refine the HiPPP CCI Framework (guided by Phases 1 and 2). Results: The HiPPP CCI Framework’s values and approaches identified in Phases 1-2 and further refined in Phase 3 were summarized under the following five key principles: 1. Inclusive, 2. Flexible, 3. Transparent, 4. Equitable, and 5. Adaptable. The framework describes values and approaches for involving consumers in research initiatives from design to translation that focus on improving healthy lifestyles and preventing obesity specifically before, during and after pregnancy; importantly it takes into consideration common barriers to partnering in obesity research during perinatal life stages, such as limited availability associated with family caregiving responsibilities. Conclusion: The HiPPP CCI Framework aims to describe approaches for implementing meaningful CCI initiatives with women in preconception, pregnancy and postpartum periods. Evaluation of the framework is now needed to understand how effective it is in facilitating meaningful involvement for consumers, researchers and clinicians, and its impact on research to improve healthy lifestyle outcomes.
Upregulation of fibronectin following loss of p53 function is a poor prognostic factor in ovarian carcinoma with a unique immunophenotype
Authors: Yokoi Ako , Matsumoto Toshihide , Oguri Yasuko , Hasegawa Yoshinori , Tochimoto Masataka , Nakagawa Mayu , Saegusa Makoto 
Published: February 2020

Abstract
Background We previously demonstrated that ovarian high grade serous carcinomas (OHGSeCa) and ovarian clear cell carcinomas (OCCCa) with an HNF-1β+/p53+/ARID1A+ immunophenotype were associated with the worst unfavorable prognosis. To clarify the molecular mechanisms underlying this finding, we focused on alterations in the p53 signaling pathway in these tumors. Methods Changes in cell phenotype and function following knockdown of wild-type p53 (p53-KD) were assessed using OCCCa cells expressing endogenous HNF-1β and ARID1A. The prognostic significance of molecules that were deregulated following p53-KD was also examined using 129 OCCCa/OHGSeCa cases. Results p53-KD cells had increased expression of Snail, phospho-Akt (pAkt), and pGSK3β, and decreased E-cadherin expression, leading to epithelial-mesenchymal transition (EMT)/cancer stem cell (CSC) features. The cells also exhibited acceleration of cell motility and inhibition of cell proliferation and apoptosis. Next generation sequencing assay revealed that fibronectin (FN) expression was significantly increased in the p53 KD-cells, in line with our observation that wild-type p53 (but not mutant p53) repressed FN1 promoter activity. In addition, treatment of OCCCa cells with FN significantly increased cell migration capacity and decreased cell proliferation rate, independent of induction of EMT features. In clinical samples, FN/p53 scores were significantly higher in OCCCa/OHGSeCa with the HNF-1β+/p53+/ARID1A+ immunophenotype when compared to others. Moreover, high FN/high p53 expression was associated with the worst overall survival and progression-free survival in OCCCa/OHGSeCa patients. Conclusion These findings suggest that upregulation of FN following loss of p53 function may impact the biological behavior of OCCCa/OHGSeCa, particularly in tumors with HNF-1β+/p53+/ARID1A+ immunophenotype, through alterations in cell mobility and cell proliferation. The accompanying induction of EMT/CSC properties and inhibition of apoptosis due to p53 abnormalities also contribute to the establishment and maintenance of tumor phenotypic characteristics.
Dramatic neurological and biological effects by Botulinum Neurotoxin Type A on SH-SY5Y neuroblastoma cells, beyond the blockade of neurotransmitter release
Authors: Wang Lei , Ringelberg Carol S. , Singh Bal R. 
Published: February 2020

Abstract
Background Gene expression profile analysis on mammalian cell lines and animal models after exposure to botulinum neurotoxin (BoNT) have been investigated in several studies in recent years. This provides a powerful tool to identify critical signaling pathways involved in the biological and inflammatory responses to BoNT and helps to determine the mechanism of the function of botulinum toxins. One of the key clinical characteristics of BoNT is its prolonged on-site effects. The role of BoNT on the blockage of neurotransmitter acetylcholine release in the neuromuscular junction has been well established. However, the effects of the treatment time of BoNT on the human cellular model and its potential mechanism remain to be defined. This study is aimed to use the gene microarray technology to compare the two physiological critical time points of BoNT type A (BoNT/A) treatment of human neuroblastoma cells and to advance our understanding of the profound biological influences that toxin molecules play in the neuronal cellular system. Results SH-SY5Y cells were treated with BoNT/A for 4 and 48 hours which represent the time needed for the entrance of toxin into the cells and the time necessary for the initial appearance of the on-site effects after BoNT application. A comparison of the two time points identified 122 functional groups that are significantly changed. The top five groups are the alternative splicing, phosphoprotein, nucleus, cytoplasm, and acetylation. Furthermore, after 48 hours there were 744 genes significantly up-regulated and 624 genes significantly down-regulated (p‹ 0.01). These genes fell into the following neurological and biological annotation groups: Nervous system development, proteinaceous extracellular matrix, signaling pathways regulating pluripotency of stem cells, cellular function and signal transduction, and apoptosis. We have also noticed that the up-regulated groups contained neuronal cell development, nervous system development, and metabolic processes while the down-regulated groups contained many chromosomes and cell cycle categories. Conclusions The effects of BoNT/A on neuronal cells extend beyond blocking the neurotransmitter release, and that BoNT/A is a multifunctional molecule that can evoke profound cellular responses which warrant a more in-depth understanding of the mechanism of the toxin’s effects after administration.
Using the Consolidated Framework for Implementation Research to describe the implementation of Patient Reported Outcome Measures (PROMs) in routine primary care
Authors: Litchfield Ian J , Greenfield Sheila , Turner Grace , Finnikin Sam , Calvert Mel 
Published: February 2020

Abstract
BackgroundThe recently announced long-term plan for the NHS is based on a model of person-centred care which relies on the sustained engagement of patients, shared decision making, and capability for self-management. It is of particular importance to a primary care service under increasing pressure from an ageing and chronically ill population. Patient Reported Outcome Measures appear capable of supporting many of the requirements of this model yet we know little of the circumstances of their current implementation or how their use might be optimised.MethodsWe conducted a series of semi-structured interviews with general practitioners across England that explored their experiences and preferences for the use of Patient Reported Outcome Measures (PROMs). We then used a post-hoc deductive analysis of the data to populate the consolidated framework for implementation research and provide a systematic description of their existing implementation and the ways in which it can be optimised and sustained.ResultsA total of 25 interviews were conducted. The Consolidated Framework for Implementation Research highlighted a number of areas that need to be addressed if PROMs are to fulfil their potential to support person-centred care. These include a lack of evidence that they offer any advantage especially if universally deployed in patients possessing a range of backgrounds, preferences and needs. Busy staff were also hesitant to produce more data that might require a response and prioritise their use in already brief consultations. A lack of training and engagement was also apparent both of which were symptomatic of the lack of a guidance in their use from within their practice, Clinical Commissioning Group or across the wider primary care system. ConclusionsThough PROMs can be readily integrated into IT systems and may help support patient engagement General Practitioners will not embrace their use until a compelling body of evidence of their benefit is created, and staff are engaged in when and how they are used. Appropriate training for staff and patients is then required funded as part of a coherent implementation strategy led by commissioning groups and policymakers.
Performance of the Upper Limb Module—A Reliable and Valid Evaluation for Chinese Patients with Duchenne Muscular Dystrophy
Authors: Chiu Yuen Yee Alice , Lo Chun Wai , Hui Chi Kuk Connie , Choi Wai Chong Susanna , Lee So Lun , Chan Hoi Shan Sophelia 
Published: February 2020

Abstract
Background Duchenne muscular dystrophy is a genetic disease leading to progressive muscle weakness and degeneration. Effective assessment tool is needed to allow monitoring of progress to guide the management. This study assessed the reliability and validity of the Performance of Upper Limb (PUL) Module when used in patients with Duchenne Muscular Dystrophy (DMD). MethodsTotal thirty-three Chinese DMD patients were included. Twenty-five video-recorded PUL Module version 1.3 assessments were performed for the recruited patients with three raters evaluated the same recorded video for inter-rater reliability and evaluated the same performance one month later for intra-rater reliability. Construct validity was assessed correlating the PUL Module scores with the patients’ age, their forced vital capacity (N=25) and their Hammersmith motor scale scores (N=25) performed on the same day. ResultsThe intra-rater and inter-rater reliability (ICC 0.92 - 0.99), internal consistency (Cronbach’s alpha 0.97 - 0.99) and known groups validity (AUC 0.97) of PUL module were excellent. PUL was negatively correlated with age (r = -0.912), and positively correlated with the forced vital capacity (r = 0.87) and the Hammersmith motor scale (r = 0.84). The findings confirm the high reliability and validity of PUL module, and its high clinical relevancy in monitoring the deteriorating upper limb motor performance that strongly correlated with the lung function and generalized motor performance as age increased in DMD. ConclusionThis first study of PUL module in Chinese patients with DMD confirmed that it is a reliable valid tool to monitor clinical progress and outcome for DMD.
First serological evidence of West Nile virus among equines and birds in Kosovo, 2018-2019
Authors: Rexhepi Agim , Sherifi Kurtesh , Berxholi Kristaq , Xhekaj Betim , Muja-Bajraktari Nesade , Özkul Aykut , von Possel Ronald , Emmerich Petra 
Published: February 2020

Abstract
Background: This study was conducted to survey the presence of the West Nile virus (WNV) in Kosovo by serological testing of the healthy autochthonous equine population and virus detection in birds and mosquitoes. Between January 2018 and June 2019, 260 equine serum samples, 626 mosquitoes (60 pools), 50 domestic birds and 51 wild birds were collected from different regions of Kosovo. Equine and domestic bird serum samples were tested by IgG ELISA while mosquitoes and bird viscera were tested for WNV RNA by RT-PCR. Positive ELISA samples were confirmed by Plaque Reduction Neutralization Test (PRNT) and eight by Virus Neutralization Test (VNT). Results: This is the first report providing evidence of WNV antibodies among animals in Kosovo. WNV antibodies were present in 27 out of 260 equine sera (10.38%) and one out of 50 samples in domestic birds by ELISA and PRNT. Eight of 27 positive equine serum samples were confirmed by VNT. No WNV RNA was detected in birds or mosquitoes.Conclusions: The occurrence of WNV antibodies in autochthonous equines from all regions of Kosovo indicates that the virus is circulating within the country. Public health authorities should therefore plan a risk assessment and disease control program.
Dickeya dianthicola emerged from a potato culture area exhibiting a wide diversity of pectinolytic pathogens in northern Morocco
Authors: Oulghazi Said , Moumni Mohieddine , Khayi Slimane , Robic Kevin , Sarfraz Sohaib , Lopez-Roques Céline , Vandecasteele Céline , Faure Denis 
Published: February 2020

Abstract
Background: Dickeya and Pectobacterium are pectinolytic pathogens that cause damage to many plants including major crops. Emergence of D. solani and re-emergence D. dianthicola were recently observed in potato fields in several continents. The purpose of this work was to describe the species diversity of Dickeya and Pectobacterium collected from potato fields in Northern Morocco, where Dickeya potato pathogens have not been isolated until recently. Results: Along three years, 119 pathogens belonging to Pectobacterium and Dickeya genera were isolated from three potato culture areas and characterized using selective PCR and gapA gene sequencing. Out of them, 19% belonged to P. versatile, 3% to P. carotovorum, 5% to P. polaris, 56% to P. brasiliense, while 17% to D. dianthicola. The taxonomic assignations were confirmed by draft genome analyses of representative isolates belonging to the collected species. D. dianthicola were isolated from a unique area where a wide species diversity of pectinolytic pathogens was also observed. In potato maceration assay, D. dianthicola isolates were more aggressive than Pectobacterium isolates, a feature that should alert stakeholders of a potential threat linked to the emergence of D. dianthicola in this country. By combining Oxford Nanopore Technologies (ONT) and Illumina technologies, the sequence of the complete genome of D. dianthicola LAR.16.03.LID was obtained. A unique circular chromosome of 4,976,211 bp codes for 4,223 predicted proteins. Comparison of the three complete genomes of D. dianthicola strains RNS049, ME23 and LAR.16.03.LID revealed a highly conserved synteny and the occurrence of strain-specific regions associated with the presence of mobile elements. Conclusion: By combining population sampling and genomics, this study highlighted the ecological context from which D. dianthicola emerged in Morocco. Furthermore, the first complete genome of a D. dianthicola strain isolated in Northern Morocco will constitute a reference genome for investigating the dynamics of the emerging D. dianthicola pathogens in this country.