bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Serial Chest Radiographs of High-Altitude Pulmonary Edema Requiring Medical Evacuation from South Pole Station, Antarctica: From Diagnosis to Recovery
Authors: Nowadly Craig D , Solomon Alex J , Burke Sean M , Rose John S 
Published: December 2020

ABSTRACT

Introduction
Chest radiography is a diagnostic tool commonly used by medical providers to assess high-altitude pulmonary edema (HAPE). Although HAPE often causes a pattern of pulmonary edema with right lower lung predominance, previous research has shown that there is no single radiographic finding associated with the condition. The majority of research involves a retrospective analysis of chest radiographs taken at the time of HAPE diagnosis. Little is known about the radiographic progression of HAPE during treatment or medical evacuation.

Materials and Methods
Three sequential chest radiographs were obtained from two patients diagnosed with HAPE at the Amundsen-Scott South Pole Station, Antarctica, who required treatment and medical evacuation. Deidentified and temporally randomized images were reviewed in a blinded fashion by two radiologists. A score of 0 (normal lung) to 4 (alveolar disease) was assigned for each of the four lung quadrants for an aggregate possible score ranging from 0 to 16 for each radiograph.

Results
Patient 1’s initial radiograph showed severe HAPE with an initial score of 13. Despite a rapid clinical improvement after medical evacuation, he continued to show multifocal radiographic evidence of disease in all the lung quadrants on day 1 (score of 11) and day 2 (score of 5). Patient 2’s radiographs showed less severe disease at presentation (score of 6). Despite the need for continued treatment, his radiographs showed a rapid improvement, with radiographic score decreasing to 3 on day 1 and 1 on day 3.

Conclusion
The chest radiographs showed serial improvement after medical evacuation in both patients. There was not a strong correlation between clinical symptoms and radiographic severity in subsequent images.
Crystal structures of the EVE-HNH endonuclease VcaM4I in the presence and absence of DNA
Authors: Pastor Michal , Czapinska Honorata , Helbrecht Igor , Krakowska Katarzyna , Lutz Thomas , Xu Shuang-yong , Bochtler Matthias 
Published: December 2020

Abstract
Many modification-dependent restriction endonucleases (MDREs) are fusions of a PUA superfamily modification sensor domain and a nuclease catalytic domain. EVE domains belong to the PUA superfamily, and are present in MDREs in combination with HNH nuclease domains. Here, we present a biochemical characterization of the EVE-HNH endonuclease VcaM4I and crystal structures of the protein alone, with EVE domain bound to either 5mC modified dsDNA or to 5mC/5hmC containing ssDNA. The EVE domain is moderately specific for 5mC/5hmC containing DNA according to EMSA experiments. It flips the modified nucleotide, to accommodate it in a hydrophobic pocket of the enzyme, primarily formed by P24, W82 and Y130 residues. In the crystallized conformation, the EVE domain and linker helix between the two domains block DNA binding to the catalytic domain. Removal of the EVE domain and inter-domain linker, but not of the EVE domain alone converts VcaM4I into a non-specific toxic nuclease. The role of the key residues in the EVE and HNH domains of VcaM4I is confirmed by digestion and restriction assays with the enzyme variants that differ from the wild-type by changes to the base binding pocket or to the catalytic residues.
Variable interplay of UV-induced DNA damage and repair at transcription factor binding sites
Authors: Frigola Joan , Sabarinathan Radhakrishnan , Gonzalez-Perez Abel , Lopez-Bigas Nuria 
Published: December 2020

AbstractAn abnormally high rate of UV-light related mutations appears at transcription factor binding sites (TFBS) across melanomas. The binding of transcription factors (TFs) to the DNA impairs the repair of UV-induced lesions and certain TFs have been shown to increase the rate of generation of these lesions at their binding sites. However, the precise contribution of these two elements to the increase in mutation rate at TFBS in these malignant cells is not understood. Here, exploiting nucleotide-resolution data, we computed the rate of formation and repair of UV-lesions within the binding sites of TFs of different families. We observed, at certain dipyrimidine positions within the binding site of TFs in the Tryptophan Cluster family, an increased rate of formation of UV-induced lesions, corroborating previous studies. Nevertheless, across most families of TFs, the observed increased mutation rate within the entire DNA region covered by the protein results from the decreased repair efficiency. While the rate of mutations across all TFBS does not agree with the amount of UV-induced lesions observed immediately after UV exposure, it strongly agrees with that observed after 48 h. This corroborates the determinant role of the impaired repair in the observed increase of mutation rate.
Supplementation of Screw-Rod C1-C2 Fixation With Posterior Arch Femoral Head Allograft Strut
Authors: Liu David D , Rivera-Lane Kendall , Leary Owen P , Pertsch Nathan J , Niu Tianyi , Camara-Quintana Joaquin Q , Oyelese Adetokunbo A , Fridley Jared S , Gokaslan Ziya L 
Published: December 2020

Abstract

BACKGROUND
Numerous C1-C2 fixation techniques exist for the treatment of atlantoaxial instability. Limitations of screw-rod and sublaminar wiring techniques include C2 nerve root sacrifice and dural injury, respectively. We present a novel technique that utilizes a femoral head allograft cut with a keyhole that rests posteriorly on the arches of C1 and C2 and straddles the C2 spinous process, secured by sutures.

OBJECTIVE
To offer increased fusion across C1-C2 without the passage of sublaminar wiring or interarticular arthrodesis.

METHODS
A total of 6 patients with atlantoaxial instability underwent C1-C2 fixation using our method from 2015 to 2016. After placement of a C1-C2 screw/rod construct, a cadaveric frozen femoral head allograft was cut into a half-dome with a keyhole and placed over the already decorticated dorsal C1 arch and C2 spinous process. Notches were created in the graft and sutures were placed in the notches and around the rods to secure it firmly in place.

RESULTS
The femoral head's shape allowed for creation of a graft that provides excellent surface area for fusion across C1-C2. There were no intraoperative complications, including dural tears. Postoperatively, no patients had sensorimotor deficits, pain, or occipital neuralgia. 5 patients demonstrated clinical resolution of symptoms by 3 mo and radiographic (computed tomography) evidence of fusion at 1 yr. One patient had good follow-up at 1 mo but died due to complications of Alzheimer disease.

CONCLUSION
The posterior arch femoral head allograft strut technique with securing sutures is a viable option for supplementing screw-rod fixation in the treatment of complex atlantoaxial instability.
Tuning osteoporotic macrophage responses to favour regeneration by Cu-bearing titanium alloy inPorphyromonas gingivalislipopolysaccharide-induced microenvironments
Authors: Xu Xiongcheng , Lu Yanjin , Zhou Ling , He Mengjiao , Zhuo Jin , Zhong Quan , Luo Kai , Lin Jinxin 
Published: December 2020

AbstractGuided bone regeneration in inflammatory microenvironments of osteoporotic patients with large alveolar bone defects remains a great challenge. Macrophages are necessary for alveolar bone regeneration via their polarization and paracrine actions. Our previous studies showed that Cu-bearing Ti6Al4V alloys are capable of regulating macrophage responses. When considering the complexity of oral microenvironments, the influences of Cu-bearing Ti6Al4V alloys on osteoporotic macrophages in infectious microenvironments are worthy of further investigations. In this study, we fabricated Ti6Al4V-Cu alloy by selective laser melting technology and used Porphyromonas gingivalis lipopolysaccharide (P.g-LPS) to imitate oral pathogenic bacterial infections. Then, we evaluated the impacts of Ti6Al4V-Cu on osteoporotic macrophages in infectious microenvironments. Our results indicated that Ti6Al4V-Cu not only inhibited the P.g-LPS-induced M1 polarization and pro-inflammatory cytokine production of osteoporotic macrophages but also shifted polarization towards the pro-regenerative M2 phenotype and remarkably promoted anti-inflammatory cytokine release. In addition, Ti6Al4V-Cu effectively promoted the activity of COMMD1 to potentially repress NF-κB-mediated transcription. It is concluded that the Cu-bearing Ti6Al4V alloy results in ameliorated osteoporotic macrophage responses to create a favourable microenvironment under infectious conditions, which holds promise to develop a GBR-barrier membrane for alveolar bone regeneration of osteoporosis patients.
Antibacterial Ti-Mn-Cu alloys for biomedical applications
Authors: Alqattan Mohammad , Peters Linda , Alshammari Yousef , Yang Fei , Bolzoni Leandro 
Published: December 2020

Abstract
Titanium alloys are common biomedical materials due to their biocompatibility and mechanical performance. However, titanium alloys are expensive and, unless surface treated, generally cannot prevent surgical infections related to bacteria which can damage the integrity of the implant. In this study, new titanium alloys were developed via powder metallurgy and the addition of manganese and copper, respectively, aiming to limit the manufacturing costs and induce new functionality on the materials including antibacterial response. The addition of manganese and copper to titanium significantly changes the behaviour of the Ti-Mn-Cu alloys leading to the successful stabilization of the beta titanium phase, great refinement of the typical lamellar structure, and achievement of materials with low level of porosity. Consequently, it is found that the mechanical performance and the antibacterial efficacy are enhanced by the addition of a higher amount of alloying elements. The manufactured Ti-Mn-Cu alloys fulfil the requirements for structural biomedical implants and have antibacterial response making them potential candidates for permanent medical implants.
Emotional news affects social judgments independent of perceived media credibility
Authors: Baum Julia , Abdel Rahman Rasha 
Published: December 2020

Abstract
How does the credibility we attribute to media sources influence our opinions and judgments derived from news? Participants read headlines about the social behavior of depicted unfamiliar persons from websites of trusted or distrusted well-known German news media. As a consequence, persons paired with negative or positive headlines were judged more negative or positive than persons associated with neutral information independent of source credibility. Likewise, electrophysiological signatures of slow and controlled evaluative brain activity revealed a dominant influence of emotional headline contents regardless of credibility. Modulations of earlier brain responses associated with arousal and reflexive emotional processing show an effect of negative news and suggest that distrusted sources may even enhance the impact of negative headlines. These findings demonstrate that though we may have distinct perceptions about the credibility of media sources, information processing and social judgments rely on the emotional content of headlines, even when they stem from sources we distrust.
Beyond the average brain: individual differences in social brain development are associated with friendship quality
Authors: Becht Andrik I , Wierenga Lara M , Mills Kathryn L , Meuwese Rosa , van Duijvenvoorde Anna , Blakemore Sarah-Jayne , Güroğlu Berna , Crone Eveline A 
Published: December 2020

Abstract
We tested whether adolescents differ from each other in the structural development of the social brain and whether individual differences in social brain development predicted variability in friendship quality development. Adolescents (N = 299, Mage T1 = 13.98 years) were followed across three biannual waves. We analysed self-reported friendship quality with the best friend at T1 and T3, and bilateral measures of surface area and cortical thickness of the medial prefrontal cortex (mPFC), posterior superior temporal sulcus (pSTS), temporoparietal junction (TPJ) and precuneus across all waves. At the group level, growth curve models confirmed non-linear decreases of surface area and cortical thickness in social brain regions. We identified substantial individual differences in levels and change rates of social brain regions, especially for surface area of the mPFC, pSTS and TPJ. Change rates of cortical thickness varied less between persons. Higher levels of mPFC surface area and cortical thickness predicted stronger increases in friendship quality over time. Moreover, faster cortical thinning of mPFC surface area predicted a stronger increase in friendship quality. Higher levels of TPJ cortical thickness predicted lower friendship quality. Together, our results indicate heterogeneity in social brain development and how this variability uniquely predicts friendship quality development.
Obsessive-Compulsive Symptoms and Other Symptoms of the At-risk Mental State for Psychosis: A Network Perspective
Authors: Ong Hui Lin , Isvoranu Adela-Maria , Schirmbeck Frederike , McGuire Philip , Valmaggia Lucia , Kempton Matthew J , van der Gaag Mark , Riecher-Rössler Anita , Bressan Rodrigo A , Barrantes-Vidal Neus , Nelson Barnaby , Amminger G Paul , McGorry Patrick , Pantelis Christos , Krebs Marie-Odile , Nordentoft Merete , Glenthøj Birte , Ruhrmann Stephan , Sachs Gabriele , Rutten Bart P F , van Os Jim , de Haan Lieuwe , Borsboom Denny ,   EU-GEI High Risk Study, Calem Maria , Tognin Stefania , Modinos Gemma , Pisani Sara , Hedges Emily , Velthorst Eva , Kraan Tamar C , van Dam Daniella S , Burger Nadine , Politis Athena , Goodall Joanne , Borgwardt Stefan , Studerus Erich , Gadelha Ary , Brietzke Elisa , Asevedo Graccielle , Asevedo Elson , Zugman Andre , Domínguez-Martínez Tecelli , Monsonet Manel , Hinojosa Lidia , Racioppi Anna , Kwapil Thomas R , Kazes Mathilde , Daban Claire , Bourgin Julie , Gay Olivier , Mam-Lam-Fook Célia , Nordholm Dorte , Randers Lasse , Krakauer Kristine , Glenthøj Louise Birkedal , Gebhard Dominika , Arnhold Julia , Klosterkötter Joachim , Lasser Iris , Winklbaur Bernadette , Delespaul Philippe A 
Published: December 2020

AbstractBackgroundThe high prevalence of obsessive-compulsive symptoms (OCS) among subjects at Ultra-High Risk (UHR) for psychosis is well documented. However, the network structure spanning the relations between OCS and symptoms of the at risk mental state for psychosis as assessed with the Comprehensive Assessment of At Risk Mental States (CAARMS) has not yet been investigated. This article aimed to use a network approach to investigate the associations between OCS and CAARMS symptoms in a large sample of individuals with different levels of risk for psychosis.MethodThree hundred and forty-one UHR and 66 healthy participants were included, who participated in the EU-GEI study. Data analysis consisted of constructing a network of CAARMS symptoms, investigating central items in the network, and identifying the shortest pathways between OCS and positive symptoms.ResultsStrong associations between OCS and anxiety, social isolation and blunted affect were identified. Depression was the most central symptom in terms of the number of connections, and anxiety was a key item in bridging OCS to other symptoms. Shortest paths between OCS and positive symptoms revealed that unusual thought content and perceptual abnormalities were connected mainly via anxiety, while disorganized speech was connected via blunted affect and cognitive change.ConclusionsFindings provide valuable insight into the central role of depression and the potential connective component of anxiety between OCS and other symptoms of the network. Interventions specifically aimed to reduce affective symptoms might be crucial for the development and prospective course of symptom co-occurrence.
Uncertainty in Divergence Time Estimation
Authors: Carruthers Tom , Scotland Robert W 
Published: December 2020

Abstract
Understanding and representing uncertainty is crucial in academic research because it enables studies to build on the conclusions of previous studies, leading to robust advances in a particular field. Here, we evaluate the nature of uncertainty and the manner by which it is represented in divergence time estimation, a field that is fundamental to many aspects of macroevolutionary research, and where there is evidence that uncertainty has been seriously underestimated. We address this issue in the context of methods used in divergence time estimation, and with respect to the manner by which time-calibrated phylogenies are interpreted. With respect to methods, we discuss how the assumptions underlying different methods may not adequately reflect uncertainty about molecular evolution, the fossil record, or diversification rates. Therefore, divergence time estimates may not adequately reflect uncertainty and may be directly contradicted by subsequent findings. For the interpretation of time-calibrated phylogenies, we discuss how the use of time-calibrated phylogenies for reconstructing general evolutionary timescales leads to inferences about macroevolution that are highly sensitive to methodological limitations in how uncertainty is accounted for. By contrast, we discuss how the use of time-calibrated phylogenies to test specific hypotheses leads to inferences about macroevolution that are less sensitive to methodological limitations. Given that many biologists wish to use time-calibrated phylogenies to reconstruct general evolutionary timescales, we conclude that the development of methods of divergence time estimation that adequately account for uncertainty is necessary. [Divergence time estimation; macroevolution; uncertainty.]
Evaluating digestibility and toxicity of native warm-season grasses for equines
Authors: Ghajar S M , McKenzie H , Fike J , McIntosh B , Tracy B F 
Published: December 2020

Abstract
Introduced cool-season grasses are dominant in Virginia’s grasslands, but their high digestible energy and nonstructural carbohydrate (NSC) levels pose a risk for horses prone to obesity and laminitis. Native warm-season grasses (NWSGs) have lower digestible energy and NSC levels that may be more suitable for horses susceptible to laminitis. Although NWSGs have desirable characteristics, they are novel forages for horses. Little is known about NWSG intake or potential toxicity to horses or how grazing by horses may affect NWSG swards. The overall objectives of this research were to 1) assess voluntary intake, toxicological response, and apparent digestibility of NWSG hays fed to horses; and 2) evaluate the characteristics of three NWSG species under equine grazing. For the first objective, a hay feeding trial using indiangrass (IG) (Sorghastrum nutans) and big bluestem (BB) (Andropogon gerardii) was conducted with nine Thoroughbred geldings in a replicated 3 × 3 Latin square design. Voluntary dry matter intake of IG and BB hays by horses were 1.3% and 1.1% of BW/d, lower than orchardgrass (Dactylis glomerata), an introduced cool-season grass, at 1.7% of BW/d (P = 0.0020). Biomarkers for hepatotoxicity remained within acceptable ranges for all treatments. Apparent dry matter digestibility (DMD) did not differ among hays, ranging from 39% to 43%. NSC levels ranged from 4.4% to 5.4%, below maximum recommended concentrations for horses susceptible to laminitis. For the second objective, a grazing trial was conducted comparing IG, BB, and eastern gamagrass (EG) (Tripsacum dactyloides) yields, forage losses, changes in vegetative composition, and effects on equine bodyweight. Nine, 0.1-ha plots were seeded with one of the three native grass treatments, and each plot was grazed by one Thoroughbred gelding in two grazing bouts, one in July and another in September 2019. IG had the greatest available forage, at 4,340 kg/ha, compared with 3,590 kg/ha from BB (P &lt; 0.0001). EG plots established poorly, and had only 650 kg/ha available forage during the experiment. Grazing reduced standing cover of native grasses in IG and BB treatments by about 30%. Horses lost 0.5–1.5 kg BW/d on all treatments. Findings suggest IG and BB merit further consideration as forages for horses susceptible to obesity and pasture-associated laminitis.
Circulating Vascular Endothelial Growth Factor and Soluble fms-Like Tyrosine Kinase-1 as Biomarkers for Endometrial Remodeling Across the Menstrual Cycle
Authors: Zolton Jessica R. , Sjaarda Lindsey A. , Mumford Sunni L. , DeVilbiss Elizabeth A. , Kim Keewan , Flannagan Kerry S. , Radoc Jeannie G. , Perkins Neil J. , Silver Robert M. , Wactawski-Wende Jean , Hill Micah J. , DeCherney Alan H. , Schisterman Enrique F. 
Published: December 2020

OBJECTIVE:
To characterize variation in circulating vascular endothelial growth factor (VEGF) and its receptor, soluble fms-like tyrosine kinase-1 (sFLT-1), across the menstrual cycle in normal ovulating women in relation to reproductive hormones to identify the utility of VEGF and sFLT-1 as peripheral biomarkers of endometrial remodeling.

METHODS:
Ninety-six healthy, regularly menstruating ovulatory women, aged 18–44 years, enrolled in the BioCycle Study, a prospective cohort study at a U.S. academic research center. Vascular endothelial growth factor and sFLT-1 were measured in concurrently collected plasma, serum, and urine up to eight times across a single cycle. Reproductive hormones were measured in serum. Mean concentrations of VEGF and sFLT-1 were compared across phases of the cycle, and correlations between specimen types were calculated. Harmonic models estimated associations between VEGF and sFLT-1 and characteristics of hormonal patterns.

RESULTS:
No variation in VEGF or sFLT-1 levels were detected over the menstrual cycle. Median (25th percentile, 75th percentile) concentrations of VEGF during the menstrual cycle were 31.2 pg/mL (24.1, 56.9) in plasma, 194.1 pg/mL (125.4, 350.2) in serum, and 101.7 pg/mL (64.2, 165.8) in urine. Plasma and serum measures were consistently correlated, whereas urinary measures were not. Vascular endothelial growth factor was not consistently associated with reproductive hormone concentrations, although sFLT-1 was associated with higher mean and amplitude of estradiol.

CONCLUSION:
Circulating VEGF and sFLT-1 did not vary across the menstrual cycle and therefore are unlikely to be useful peripheral biomarkers of endometrial changes across the menstrual cycle. For studies measuring circulating VEGF for other reasons, plasma may be the preferred medium and timing to menstrual cycle phase need not be considered for reproductive-age women.