bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-874 ameliorates retinopathy in diabetic rats by NF-κB signaling pathway
Authors: Li Rui , Yuan Huimin , Zhao Tao , Yan Yimin , Liu Zhaochen , Cai Jiayu , Qiu Chunli , Li Chuanjing 
Published: January 2020

Abstract
Background: This study aimed to explore the improvement effect of miR-874 on retinopathy in diabetic rats by NF-κB signaling pathway. Methods: Ten healthy Sprague-Dawley rats were taken as the control group. Sixty streptozotocin (60 mg/kg)-induced diabetes model rats were randomly divided into model group (without any treatmentinjection of normal saline), negative control (NC) agomir group (injection of overexpressed NC vector), miR-874 agomir group (injection of miR-874 mimic), miR-874 anti-agomir group (injection of miR-874 inhibitor), EVP4593 group (injection of NF-κB signaling pathway antagonist EVP4593), and miR-874 anti-agomir + EVP4593 group (injection of miR-874 inhibitor and EVP4593). All injection was via caudal vein. Results: miR-874 could target the degradation of p65. Compared with control group, there were significantly reduced miR-874 expression, increased VEGF and Ang2 protein expressions, lowered end-diastolic velocity and peak systolic velocity of central retinal artery (CRA), and blood velocity of central retinal vein and CRA, heightened plasma viscosity, blood viscosity and erythrocyte sedimentation rate at all shear rates, decreased capillary pericytes, increased vascular endothelial cells, and ascended p65 expression in the retina of rats in model group (all P < 0.05). It showed that pathological changes were happened on the retina of diabetes rats. These indexes showed the same results after miR-874 was inhibited (all P < 0.05). However, these indexes showed the opposite results in miR-874 agomir group and EVP4593 group compared with model group (all P < 0.05). EVP4593 could alleviate the aggravation of retinopathy caused by the inhibition of miR-874 in diabetes rats. Conclusions: miR-874 mediates NF-κB signaling pathway by targeting the degradation of p65 to further improve the retina of diabetes rats, showing the improvement effect of miR-874 on diabetic retinopathy in rats.
Detection of Atypical porcine pestivirus in Swedish pigs with congenital tremor type A-II
Authors: Stenberg Hedvig , Jacobson Magdalena , Malmberg Maja 
Published: January 2020

Abstract

Background Congenital tremor (CT) type A-II is a neurological disorder characterized by tremor of the head and body of new-born piglets. The suggested causative agent of the disease is the recently found atypical porcine pestivirus. The virus has been detected in piglets suffering from congenital tremor in central Europe, South and North America and in China but no studies has so far been performed in the Nordic countries. The overarching goal of this study was to investigate if atypical porcine pestivirus are present in the brain tissue of Swedish piglets suffering from congenital tremor. From June 2017 – June 2018, 15 piglets from four Swedish farms with ongoing outbreaks of CT and 13 piglets with splay leg originating from four different farms, were investigated for presence of APPV RNA in brain tissue. Matched healthy control piglets (n=8) were also studied. Two APPV-specific RT-qPCR methods targeting the NS3 and NS5B region, respectively, were used. A retrospective study was performed on material from Swedish piglets with CT sampled in 2004 (n = 11) and 2011/2012 (n = 3) using the described APPV-specific RT-qPCR methods. The total number of piglets with signs of CT in this study was 29. Results Atypical porcine pestivirus-RNA was detected in 93% (27/29) of the piglets suffering from congenital tremor. All piglets with congenital tremor from 2004 (n = 11) and 2012 (n = 3) were PCR-positive with respect to APPV, whereas, all of the healthy controls (n = 11) were negative. The piglets with CT sampled 2017-2018 had an odds ratio of 271 (95% CI 12.1 to 6096.8, z = 3.5, P = 0.0004) to test positive for APPV by qRT-PCR compared to the healthy piglets (Fishers exact test p < 0.0001). These findings make it interesting to continue investigating APPV in the Swedish pig population. Conclusion This is the first description of atypical porcine pestivirus in piglets suffering from congenital tremor type A-II in Sweden and the Nordic countries. The virus has been present in the Swedish pig population since at least 2004. Keywords: congenital tremor, type A-II, atypical porcine pestivirus, splay legs, Sweden, pigs, piglets.
Elucidation of molecular mechanisms of flower form development in tree peony ( Paeonia suffriticosa ) through comparative transcriptome analysis of floral parts
Authors: Lu Jiuxing , Zheng Yun , Wang Haoning , Wang Zheng , Li Yonghua , Gao Gary , Li Yan 
Published: January 2020

Abstract
Background: Tree peony ( Paeonia suffruticasa ) is an economically, medicinally and ornamentally important woody flowering plant in East Asia. It is also a common ornamental shrub in Europe and North America. They are well known and prized for their beautiful flowers in many different shapes. Stamen petalody has been shown to be the most effective way to modify flower shapes. However, there is limited information on the molecular mechanisms of stamen petalody and flower shape formation in tree peony. Results: In this study, RNA sequencing was used to assemble and annotate the unigenes in tree peony to identify the critical genes related to flower parts formation and verify the key genes in different flower shapes of tree peony cultivars. A total of 76,007 high quality unigenes were assembled and 30,505 were successfully annotated. A total of 1,833 transcription factors (TFs) were identified in our study, among them 16 MADS-box genes were found and characterized. Six key genes were selected to verify their functions in stamen petalody. AG and SEP showed high expression level in carpels and sepals separately both in stamen petalody and non-stamen petalody groups. PI and AP3 s howed higher expression levels of inter-petals in the stamen petalody group, compared to stamens of non-stamen petalody. Conclusion: S ixteen MADS-box genes were identified in tree peony through RNA-seq. We identified six key genes based on their differential expression levels in different flower parts. These six key genes represented all categories in the ABCDE model to verify the functions in stamen petalody. We speculate that PI and AP3 may trigger the stamen petalody in tree peony. Our study has helped establish the flower development model in tree peony, to identify key molecular mechanisms in the development of different flower shapes, and to provide valuable information for improving the genetic diversity of tree peony and many other woody plants.
Effect of continuous administration of low-medium flow oxygen for non-ventilated lung during one lung ventilation surgery on blood gas analysis and oxidative stress response in lung tissue of tumor patient：A randomized controlled trial
Authors: Wu Tang-Jing , Zhan Jia , Ke Jian-Juan , Zhang Zong-Ze , Wang Yan-Lin 
Published: January 2020

Abstract
Background: One-lung ventilation (OLV) induces hypoxia during the operation and oxidative stress to the non-ventilated lung (NVL) which may cause acute lung injury (ALI). Aims: We sought to find out whether continuous administration of low-medium flow oxygen for NVL during OLV can mitigate the oxidative stress in lung tissue of patient. Methods: After local Ethics Committee approval and informed consent, we randomly allocated fifty-seven patients scheduled for elective pulmonary tumor resection. The F14 tube was placed at 2-3cm beyond the carina of trachea in the NVL at the time of the beginning of the OLV in group O, administrating continuously with oxygen. Blood samples were taken from radial artery and internal jugular vein simultaneously for blood gas analysis, immediately after induction of anesthesia (T 1 ), 30min (T 2 ), 1h (T 3 ) and 2h (T 4 ) after OLV. Lung tissue was taken 5cm distant from the tumor for determination of superoxide dismutase(SOD) and malondialdehyde(MDA), and expression of heme oxygenase-1(HO-1) . Result: Compared with group C, the PaO 2 was significantly increased at T 2-4 and the PaCO 2 was significantly decreased at T 2,3 , the PvO 2 was significantly increased at T 2,3 and the PvCO 2 was significantly decreased at T 2-4 , the lung concentration of MDA was significantly lower and the local expression level of HO-1 in lung was significantly elevated in group O （ P <0.05）. Conclusion: The administration of continuous low-medium flow oxygen for NVL during OLV has shown the effect of pulmonary protection. The possible mechanism is related with the inhibition of the oxidative stress response in lung tissue.
Translation and adaptation of the Learning Organisation Survey for french speaking countries.
Authors: Diez Amalia Giuliani , Amine Souadka , Majbar Mohammed Anass , Benkabbou Amine , Mohsine Raouf , Akhnif El Houcine 
Published: January 2020

Abstract
Background Learning organizations need a diagnostic tool that allows them to assess how well their team, department, or the entire institution is performing, and help them identify areas of improvement. In turn, having that tool in your own language (in this case, French) and adapted to your own context, gives reliability and ease of application. Health organizations are starting to use more and more tools like this that allow them to improve their learning capabilities and, therefore their performance. The aim of the study was the translation of Garvin's et al. Learning Organization Survey into French to evaluate french-speaking health organizations. Methods the method used to translate the questionnaire was based on Brislin's modified reverse translation technique. Followed by the stage of cross-cultural adaptation through an Atelier of experts, obtaining a pre-final version that was reviewed by a committee of judges with an index of the validity of IVC content ≤ 80%. The French version of the questionnaire proved to be an adequate instrument for the evaluation. The survey was conducted with a sample of 111 workers from the National Oncology Institute of Rabat, who accepted the filling of the survey, ≥ 65%. Results The established validation criteria were exceeded with results > 80% in the assessment of experts. We found a good internal consistency, evidenced by a Cronbach's Alpha of > 0.8 in each block. Conclusion The questionnaire gathers enough psychometric properties to be considered a useful and reliable tool capable of objectively measuring a french speaking Learning Organization.
Hydrogen attenuates postoperative pain through Trx1/ASK1/MMP9 signaling pathway
Authors: Li Juan , Zhang Li , Li Qian , Yang Xing , Teng Peng , Peng Qilin , Shi Yadan , Yu Pan , Hu Liang , Liu Wentao , Duan Manlin 
Published: January 2020

Abstract
Background: Postoperative pain is a serious clinical problem with a poorly understood mechanism and lack of effective treatment. Considering hydrogen (H 2 ) could reduce neuroinflammation, we hypothesizes that hydrogen may alleviate postoperative pain and investigate the mechanism. Methods: Mice were used to establish postoperative pain model via performing a plantar incision surgery. Mechanical allodynia was measured using the Von Frey test. Cell signaling was assayed using gelatin zymography, western blotting, immunohistochemistry and immunofluorescence staining. The animals or BV-2 cells were received with/without ASK1 and Trx1 inhibitor to investigate the effects of H 2 on microglia. Results: Plantar incision surgery significantly decreased the mechanical threshold, and increased MMP-9 activity and ASK1 phosphorylation in the spinal cord of mice. MMP-9 knockout and ASK1 inhibitor NQDI-1 attenuated postoperative pain. H 2 treatment increased Trx1 expression, decreased ASK1, p38 and JNK phosphorylation, MMP-9 activity, pro-IL-1β maturation and IBA-1 expression in the spinal cord of postoperative pain mice, and ameliorated postoperative pain. In vitro, H 2 increased Trx1 expression and reduced MMP-9 activity induced by LPS in BV-2 cells. Additionally, H 2 also reduced ASK1, p38 and JNK phosphorylation, and IBA-1 expression induced by LPS, which were abolished by Trx1 inhibitor PX12 in BV-2 cells. Conclusions: For the first time the results confirm that H 2 can work as a therapeutic agent for ameliorating postoperative pain through Trx1/ASK1/MMP9 signaling pathway. MMP-9 and ASK1 may be the targeting molecules to relieve postoperative pain.
Causes and Predictors of Mortality among HIV-Exposed Infants in Rural North-Central Nigeria: Results of a Simple Verbal Autopsy Survey
Authors: Ozigbu Chamberline E. , Erekaha Salome , Chinaeke Eric E. , Jasper Tongdiyen L. , Nwanne Gift , Bathnna Miriam , Nachega Jean B. , Isaac Elon W. , Sam-Agudu Nadia A. 
Published: January 2020

Abstract
Background HIV-exposed infants (HEI) who die before diagnosis or treatment initiation, or who die in spite of being HIV-free constitute missed opportunities for reducing infant mortality. Verbal autopsy (VA) has been successfully applied in the collection of data to determine symptoms and circumstances surrounding death among infants, children and adults among populations that lack vital registration systems. There is little available data on rates and causes of death among HIV-exposed infants (HEI) in Nigeria. We used VA to characterize attributable causes and predictors of mortality among HEI in rural North-Central Nigeria.Methods Pregnant women living with HIV and HEI were enrolled at rural primary healthcare facilities and followed-up for 12 months, post-delivery. A simple 21-item VA instrument was used to collect infant mortality information from mothers, other family members, mentor mothers, and/or healthcare workers. Attributable causes of death were determined by physician coding. Multivariate logistic regression was performed to determine independent predictors of mortality.Results Data from 455 HIV-exposed infected and uninfected fetus/infant-mother pairs were analyzed. All mothers received anti-retroviral therapy. Seventy-five (16.5%) fetuses/infants died during gestation and within 12 months post-delivery. Forty (53.3%) deaths occurred in utero . The 12-month infant mortality risk among HEI in our study was 88.7/1,000. Among the 35 live-born infants, birth asphyxia (6/17, 35.3%) and sepsis (7/18, 38.9%) were the most common causes of death in the neonatal and post-neonatal periods, respectively. Unadjusted estimates showed that a greater proportion of deceased infants had mothers who did not deliver at a health facility (53.3 vs 31.8%, p=0.003), and who were newly HIV-diagnosed during pregnancy (69.3 vs 50.8%, p=0.029). Infants receiving nevirapine prophylaxis within 72 hours were less likely to have died (aOR = 0.40, 95% CI: 0.2-0.9).Conclusions Early HIV diagnosis and treatment among women of child-bearing age, maternal access to facility delivery and timely infant antiretroviral prophylaxis should be programmatically strengthened to reduce HEI mortality. Additionally, robust monitoring and evaluation systems are needed to track and record deaths among HEI.
Systematic analysis of factors that improve HDR efficiency in CRISPR / Cas9 technique
Authors: Foschi Nicola , Athanasakis Emmanouil , Gasparini Paolo , Stazio Mariateresa Di , d’Adamo Adamo Pio 
Published: January 2020

Abstract
The bacterial CRISPR/Cas9 system has a proven to be an efficient tool for genetic manipulation in various organisms, but the efficiency of sequence replacement by homologous direct repair (HDR) is substantially lower than random creation of indels. Many studies focused on improving the efficiency of HDR using double sgRNA, cell synchronization cycle and the delivery of ssODN with a rational design. In the present study, we tested and compared the combination of these three methods to improve HDR efficiency. To our tests, we chosen the TNFα gene (NM_000594) for its crucial role in a variety of biological processes and diseases. Our results showed a dramatically increases of HDR efficiency from undetectable HDR event to 39% of HDR efficiency and provide a new strategy to facilitate CRISPR/Cas9-mediated human genome targeting. Furthermore, we showed that TNFα gene could be edited with CRISPR/Cas9 methodology, an opportunity to safely correct, in the future, the specific mutations of each patient.
Clinical application of one-position complete retroperitoneal laparoscopic radical resection of renal pelvis and ureteral carcinoma
Authors: Xiao Yongshuang , Du Wei , Hou Yulong , Li Hailong , Li Shuofeng , Wen Rumin 
Published: January 2020

Abstract
Background: To explore the surgical technique and the clinical efficacy of complete retroperitoneal laparoscopic radical resection in patients with upper tract urothelial carcinoma (UTUC).Methods: A total of 11 patients (6 males and 5 females) newly diagnosed with UTUC with T1/T2 were enrolled in this study who underwent one-position complete retroperitoneal laparoscopic radical resection in our hospital from March 2016 to December 2018. Among them, 8 cases were of renal pelvis carcinoma and 3 cases were of ureteral carcinoma. The average age was 57.5 years (range, 44-78 years). All patients were presented with painless gross hematuria. CTU and ureteroscopy were used to diagnose.Results All the 11 patients had successful operations without open transition. The mean operating time was 85 min (range 60 to 115 min). The mean amount of intraoperative bleeding was below 90 ml. The postoperative hospital stay was 3-7 days, with an average of 5 days. There was no obvious complication after operation. No local tumor recurrence or new bladder implantation metastasis has already been observed with the postoperative follow-up time in 8 to 40 months.Conclusion One-position complete retroperitoneal laparoscopic radical resection of UTUC is safe and feasible. The curative effect is affirmative. It has the conspicuous advantages in less bleeding during operation, minimally invasive, no posture change, quicker recovery with little disturbance of abdominal organ and shorter postoperative hospital stay, which could particularly reduce workload of operating room nurses, without any extra postoperative complications.
Trends of Muscle Invasive Bladder Cancer: Evidence from the SEER Database, 1988 to 2013
Authors: Chalfant Victor , Blute Michael L. , Zahra Hadi , Silberstein Peter 
Published: January 2020

Abstract
Background: Guidelines for Muscle-Invasive Bladder cancer (MIBC) recommends that patients receive neoadjuvant chemotherapy with radical cystectomy or chemoradiotherapy, as treatment over radical cystectomy alone. Though trends and practice patterns of MIBC have been defined using the National Cancer Database, data using the Surveillance, Epidemiology, and End Results (SEER) have been poorly described. Methods: Using the SEER database, we collected data of MIBC according to the AJCC (American Joint Commission on Cancer). We considered differences in patient demographics and tumor characteristics based on three treatment groups: chemotherapy with radical cystectomy, radical cystectomy alone, and chemoradiotherapy. Multinomial logistic regression was performed to compare likelihood ratios. Temporal trends of each treatment were compared. Results: Of 16,728 patients, 10,468 patients received radical cystectomy alone, 3,236 received radical cystectomy plus chemotherapy, and 3,024 received chemotherapy plus radiation. Patients who received chemoradiotherapy tended to be older and African American compared to radical cystectomy alone (<.001); stage III patients tended to be divorced (<.001). Patients who received chemotherapy with radical cystectomy tended to be males (<.001); stage II patients were less likely to be Asian than White (<.001). Stage III patients were less likely to receive chemoradiotherapy as a treatment option than stage II (<.001). Chemotherapy with radical cystectomy and chemoradiotherapy are both used less frequently as treatment, though increasingly utilized (<.001). Conclusion: Radical cystectomy alone is still the most commonly used treatment for muscle-invasive bladder cancer based on temporal trends. Significant disparities exist in those who receive chemoradiotherapy over radical cystectomy for treatment.
The prevalence of Adverse Drug Events in septic patients after Rapid Response Team Activation
Authors: QAHTANI SAAD AL 
Published: January 2020

Abstract
Background: Antibiotics are commonly prescribed in hospitals, some use are unnecessary or inappropriate, which may increase Adverse Drug Events (ADEs) rate. ADEs can be a leading cause of morbidity and mortality, despite this; it is receiving little attention. A meta-analysis done by Lazarou. J and colleagues, estimated that more than 2 million (6.7%) of hospitalized patients have a serious adverse drug reaction (ADR) with a mortality rate of 0.32%, means ADR causing over 100,000 deaths annually. This study was conducted to evaluate the prevalence of antibiotics ADEs in Critical Care Response Team (CCRT) activated patients. Methods Single-center, randomized, prospective observational cohort study took place at an academic tertiary hospital in Riyadh, Saudi Arabia. All adult patients who received antibiotics were included in the study. ADE was defined as any recognized / possible injury associated with antimicrobials use and were evaluated post CCRT (RRT) activation. Results A total of 315 patients with a mean age of 61.1±20.4 who received antibiotics mainly for pneumonia included in the study. 29.5% experienced at least one ADE through the study period. The most frequently common ADE were renal in 12.4%, followed by the hematological adverse events in 11.4% of the patients. Conclusion We found that antibiotics –associated ADEs were common in CCRT patients, minimizing the unnecessary use of antibiotics, following hospital antibiotic guideline and applying other necessary measures can decrease ADEs morbidity rate.
Mapping the Trends in Esophageal Cancer Disease Burden in the United States: Results from the Global Burden of Disease Study 2017
Authors: Lu Di , Zhan Jintao , Liu Xiguang , Dong Xiaoying , Feng Siyang , Zhai Jianxue , Mai Shijie , Jiang Jianjun , Wang Zhizhi , Ji Xiuyu , Li Mei , Wu Hua , Cai Kaican 
Published: January 2020

Abstract
Background: Esophageal cancer is the 7th leading cancer globally and the 10th leading cancer in the United States. However, it is has received limited attention over more common malignancies. Only a few studies have comprehensively assessed disease burden from esophageal cancer in the United States (US). Methods: Using states-categorized data on incidence, mortality, and Disability-adjusted Life Years (DALYs), this study analyzed the current trends in esophageal cancer disease burden. Data and risk factor indicators were obtained from Global Burden of Disease (GBD) online resource and used to determine annual relative change. Results: We report here that between 1990 and 2017, the number of esophageal cancer new cases, deaths and DALYs in the US increased significantly, while the Age-standardized Rate (ASR) of disease incidence remained constant. During the same time, disease burden from esophageal cancer in males was higher than that in females. Economically stronger states trend to had lesser disease burden from esophageal cancer. Smoking and alcohol use contributed most of the burden while influence of high body-mass index and diet low in fruits grew largely. Conclusions: This study provided an analysis of esophageal cancer disease burden in the United States that will inform the design of targeted strategies for disease prevention tailored to different states.