bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANLN&nbsp;Promotes Carcinogenesis in Oral Cancer by Regulating PI3K/mTOR&nbsp;Signaling Pathway
Authors: Wang Bing , Zhang Xiaoli , Li Chenxi , Liu Ningning , Hu Min , Gong Zhongcheng 
Published: October 2020

Abstract
Background: Oral cancer is a malignant disease threatening human’s life and greatly reduces human’s life quality. ANLN was reported to promote progression in cancer. This study aims at investigating the role of ANLN and the molecular mechanism in oral cancer. Methods: ANLN was down-regulated by RNAi technology. The effect of ANLN on cell behaviors including proliferation, cycle distribution, invasion, and apoptosis was detected. Western blotting analysis was used to explore the mechanism of ANLN in oral cancer. Results: Based on data from TCGA database, ANLN was shown to be expressed significantly higher in tumor tissues than the normal control. Patients with higher ANLN expression exhibited shorter survival time. ANLN was also expressed abundantly in cancer cell lines CAL27 and HN30. When ANLN was knocked down in CAL27 and HN30 cells, cell proliferation and colony formation ability was downregulated. Cell invasion ability was also suppressed. But cell apoptosis rate was induced reversely. In addition, the level of critical members in PI3K signaling pathway including PI3K, mTOR, Akt, and PDK-1 were significantly reduced after ANLN was knocked down in CAL27 cells. Conclusions: ANLN contributes to progression in oral cancer and affects activation of PI3K/mTOR signaling pathway. This study provides a new potential target for therapy and drug development in oral cancer.
Zinc and Vitamin a Deficiency Predisposes to the Need for Intubation and Icu Admission in Patients With COVID-19. An Observational Study
Authors: Berrocal Lara Bielsa , Irriguible Teresa Tomasa , Philibert Viridiana , Llàcher Cristina Tural , Osaba Jaume Barallat Martínez de , Domínguez Josep Maria Manresa , Monserrat Pere Torán 
Published: October 2020

Abstract
Objective: To analyse whether the micronutrient deficit predicts a worse prognosis in patients infected by SARS-CoV-2 who developed the COVID-19 disease.Methods: We carried out an observational and retrospective study with 120 patients admitted for COVID-19 in the ICU and in the Internal Medicine ward of a tertiary hospital. In the nutritional admission analysis of these patients, we analysed plasmatic levels of vitamins A, B6, C, D, E and zinc. In addition, different variables of interest were collected, such as the need for orotracheal intubation, hospital stay, mortality and multi-organ failure.Results: One hundred and twenty patients were included. The independent variables associated with the need for ICU admission were low levels of zinc (standard error 0.566, 95% CI 0.086 to 0.790, p = 0.017), low levels of vitamin A (standard error 0.582, 95% CI 0.061 to 0.594, p = 0.004), age over 65 (standard error 0.018, 95% CI 0.917 to 0.985, p = 0.005) and male gender (standard error 0.458, 95% CI 1.004 to 6.040, p = 0.049). The only variable that was independently associated with the need for orotracheal intubation was vitamin A deficiency (standard error 0.58, 95% CI 0.042 to 0.405, p = 0.000).Conclusions: Low levels of vitamin A and zinc are associated with a greater need for admission to the ICU and orotracheal intubation. Patients older than 65 years had higher mortality. Randomized clinical trials are needed to examine whether micronutrient supplementation could be beneficial as an adjunctive treatment in COVID-19.
Mesenchymal Stromal Cells as Prophylaxis for Graft-versus-host Disease in Haplo-identical Hematopoietic Stem Cell Transplantation Recipients With Severe Aplastic Anemia? - A Systematic Review and Meta-analysis
Authors: Li RuoNan , Tu Jingke , Zhao Jingyu , Pan Hong , Fang Liwei , Shi Jun 
Published: October 2020

Abstract
Background: Mesenchymal stromal cells (MSCs) are an emerging prophylaxis option for graft-versus-host disease (GVHD) in haplo-identical hematopoietic stem cell transplantation (haplo-HSCT) recipients with severe aplastic anemia (SAA), but studies have reported inconsistent results. This systematic review and meta-analysis evaluates the efficacy of MSCs as prophylaxis for GVHD in SAA patients with haplo-HSCT. Methods: Studies were retrieved from PubMed, EMBASE, Cochrane, Web of Science and http://clinicaltrials.gov from establishment to February 2020. Twenty-nine single-arm studies (n = 1456) were included, eight (n = 241) studies combined with MSCs and eleven (n = 1215) reports without MSCs in haplo-HSCT for SAA patients. The primary outcomes were the incidences of GVHD. Other outcomes included 2-year overall survival (OS) and the prevalence of cytomegalovirus viremia (CMV). Odds ratios (ORs) were calculated to compare the results pooled through random or fixed effects models. Results: Between MSCs and no MSCs groups, no significant differences were found in the pooled incidences of acute GVHD (56.0%, 95%CI: 48.6%-63.5% vs. 47.2%, 95%CI: 29.0%-65.4%; OR 1.43, 95%CI: 0.91-2.25; p = 0.123), grade II-IV acute GVHD (29.8%, 95%CI: 24.1%-35.5% vs. 30.6%, 95%CI: 26.6%-34.6%; OR 0.97, 95%CI: 0.70-1.32; p = 0.889), chronic GVHD (25.4%, 95%CI: 19.8%-31.0% vs. 30.0%, 95%CI, 23.3%-36.6%; OR 0.79, 95%CI 0.56-1.11; p = 0.187). Furtherly, there was no obvious differences in 2-year OS (OR 0.98, 95%CI 0.60-1.61; p = 1.000) and prevalence of CMV (OR 0.61, 95%CI 0.40-1.92; p = 0.018).Conclusions: Our meta-analysis indicates that the prophylactic use of MSCs co-transplantation is not an effective option for SAA patients undergoing haplo-HSCT. Hence, the general co-transplantation of MSCs for SAA haplo-HSCT recipients may lack of evidence-based practice.
TAZ Regulates Bladder Cancer Cell Proliferation and Apoptosis via p38 Activity
Authors: Sun Yangyang , Hu Lianxin , Liu Chengxi , Su Ganglin , Yang Lina , Huang Weiren , Chen Wei 
Published: October 2020

Abstract
Background The progression of cancer is driven by the deregulation of various signaling pathways, especially Hippo and p38 MAPK pathway. TAZ, a downstream target of Hippo pathway, has been demonstrated to promote tumorigenesis in various cancers, but the functions of both Hippo and p38 signaling in bladder cancer cells are still unclear. Methods T24 and 5637 cells with knockdown of TAZ were constructed. EdU cell proliferation assay and western blot were used to illustrate the effects of TAZ on the proliferation and apoptosis of bladder cancer cells and the expression of p38 protein and phosphorylation. We overexpressed Flag-tagged TAZ in 293T cells. Western blot and RT-qPCR were used to further illustrate the effect of TAZ on the expression level of p38. The p38 inhibitor (PH-797804) combined with western blot and EDU cell proliferation assay were used to show whether TAZ affects the proliferation and apoptosis of bladder cancer cells by regulating the activity of p38.Results The shTAZ contained in T24 and 5637 cells significantly inhibited bladder cancer cells proliferation, in addition, the knockdown of TAZ-induced apoptosis of T24 and 5637 cells was found out. The loss of TAZ led to the upregulation of p38 protein as well as phosphorylation. The over-expression of Flag-TAZ had no obvious effect on p38 mRNA level, but p38 protein was reduced clearly in 293T cells. ShTAZ induced the upregulation of cleaved-caspase 3, which disappeared when treated with PH-797804, a p38 inhibitor, and the reduction to EdU positive cells induced by shTAZ was reversed by PH-797804 treatment, which suggested that p38 activity could mediate both cell proliferation and apoptosis regulated by TAZ knockdown. Conclusions In this study, it was demonstrated that TAZ could regulate the proliferation and apoptosis of bladder cancer cells by regulating the stability of p38 protein. Our finding uncovered the novel functional interaction between Hippo and p38 MAPK pathway. An in-depth understanding of this question may indicate a new direction of diagnosis or treatment for bladder cancer.
Application of Fluorescence in Situ Hybridization to Detect MYCN Amplification on Paraffin-embedded Tissue Sections of Neuroblastoma - Single Institute Study.
Authors: Winnicka Dorota , Babicz Mariusz , Styka Borys , Skowera Paulina , Zawitkowska Joanna , Lejman Monika 
Published: October 2020

Abstract
BackgroundNeuroblastoma is the most common extracranial solid tumour in children. Amplification of the MYCN gene was observed in approximately 20-30% of tumours. It is strongly correlated with advanced stage of disease, rapid tumour progression, resistant to chemotherapy and poor outcome independent of patient age and stage of advanced disease. Amplification of the MYCN identify high-risk patients.MethodsTo assess neuroblastoma tumours with MYCN amplification we used paraffin-embedded tissue sections in 57 patients by fluorescent in situ hybridization (FISH). ResultsTwenty-eight (49,1%) of patients had localized neuroblastoma (1–3 stages), 5,3% stage 4S, and 45,6% stage 4 disease. Thirty-eight (66,7%) of patients had got abdominal/adrenal mass at diagnosis. Twenty-seven (47,4%) patients were <18 month of age, thirty (52,6%) patients were >18 month of age. MYCN amplification appeared in 12 (21%) tumour specimens. ConclusionsFluorescent in situ hybridization is a high-sensitive, useful technique for detecting MYCN amplification on paraffin- embedded tissue section of neuroblastoma tumours thus facilitating therapeutic decisions based on the presence or absence of this important biologic marker.
Extended Ischiopubic Fixation Using Porous Metal Augments In Cementless Acetabular Reconstruction During Revision Total Hip Arthroplasty
Authors: Huang Yong , Zhou Yixin , Shao Hongyi , Tang Hao , Yang Dejin , Zhou Baochu 
Published: October 2020

Abstract
Background: As opposed to rim fixation, 3-point fixation provides sufficient primary stability for cememless cups in the presence of massive bony deficiency. While several methods, including doming augments or flying buttress augments, have been introduced to reconstruct superior supportive points, good methods to establish supportive ischiopubic points are currently lacking. The aims of this study were to introduce the definition, indication and surgical technique of extended ischiopubic fixation (EIF) and to investigate the early clinical and radiographic results and complications of hips revised with EIF.Methods: Patients who underwent revision THA utilizing the technique of EIF with porous metal augments secured in the ischium and/or pubis in a single institution from February 2015 to February 2020 were retrospectively reviewed. 25 patients were included based on the criteria of minimum 2-year follow-up. The median duration of follow-up was 48.06 months (interquartile range: 41.37-62.20 months). The patients’ clinical results were assessed using of the Harris Hip Score and Western Ontario and McMaster Universities Osteoarthritis index and any complications including infection, intraoperative fracture, dislocation, postoperative nerve palsy, hematoma, wound complication and/or any subsequent reoperation(s) were documented. The radiographic results were assessed with the construct stability, the position of the center of rotation and cup orientoration. Results: At the most recent follow-up, 20 (80%) patients rated their satisfaction level as “very satisfied”, 5 (20%) were “satisfied”. The median HHS score improved from 36.00 preoperatively to 81.00 (p＜0.001) while the median WOMAC index score decreased from 74.50 preoperatively to 16.50 at the latest follow-up (p＜0.001). No intraoperative or postoperative complications were identified. All constructs were considered to have obtained stable fixation. The median vertical and horizontal distance between the latest postoperative center of rotation to the anatomic center of rotation improved from 13.62 mm and 8.68 mm preoperatively to 4.42 mm and 4.19 mm, at final follow-up (p<0.001), respectively.Conclusions: Early follow-up of patients reconstructed with porous metal augments using the EIF technique demonstrated satisfactory clinical results with no particular complications, stable fixation and restoration of the center of rotation.
The Association Between Dietary Salt Intake and the Glycaemia Response Among Type 2 Diabetes Patients in Eastern China
Authors: Lin Yi , Yang Xi , Chen Yan-Shu , Zhou Ye , Li Li 
Published: October 2020

Abstract
BackgroundType 2 diabetes (T2D) is one of public health concerns in China with a rapid increase in prevalence. The study on salt intake and risk of T2D is still lack in China.AimsTo investigate the association between dietary salt intake levels and the glycaemia response of T2D.MethodsA total of 1145 T2D patients, who accepted standardized management by the National Standardized Metabolic Disease Management Center at Ningbo First Hospital from March 2018 to January 2020, were selected in the final analysis. Demography, lifestyle and medical information were collected through questionnaires. Anthropometry, blood pressure and biomarkers were measured by well-trained endocrinology nurses. Generalized linear models (GLM) were used to examine the association.ResultsHigher prevalence of overweight and central obesity with larger BMI and waist circumference were found in higher salt categories 6-8g/d and ≥8g/d, compared to lower salt categories. Fasting plasma glucose (FPG), 2h postprandial plasma glucose, 2h postprandial insulin, total cholesterol, triglyceride and low-density lipoprotein cholesterol were significantly different across salt intake categories. GLM further shows that salt intake 6-8g/d and ≥8g/d were positively associated with FPG and salt intake 6-8g/d and ≥8g/ d was associated with HbA1c.ConclusionIncreasing salt intake is suggested to be associated with the glycaemia response at the fasting state in T2D patients. Hospital-based education is needed for improvement of awareness, attitude and action on restriction of salt consumption.
Combined Primary Hepatic Neuroendocrine Carcinoma and Hepatocellular Carcinoma: Case Report and Literature Review
Authors: Nakano Akira , Hirabayashi Kenichi , Mashiko Taro , Masuoka Yoshihito , Yamamoto Seiichiro , Ozawa Soji , Nakagohri Toshio 
Published: October 2020

Abstract
Background: Hepatocellular carcinoma (HCC) can grow in a mosaic pattern, often combined with various non-hepatocellular cells. However, HCC combined with a neuroendocrine carcinoma (NEC) component is very rarely reported, and its clinical features, origin, diagnosis and behavior have not been established. In the literature, mixed HCC–NEC tumors are categorized as either collision type or combined type, depending on their microscopic features. Here we report a patient with a combined-type HCC–NEC tumor.Case presentation: An asymptomatic 84-year-old woman was found to have a solid mass in the right lobe of her liver. Laboratory and radiologic examinations showed findings typical of HCC, including arterial-phase enhancement, and portal- and delay-phase washout. She was preoperatively diagnosed with HCC, and treated with partial laparoscopic hepatectomy of her S5 segment. The tumor was 5.0 × 6.0 cm in diameter, and was predominantly HCC, partly admixed with a NEC component. A transitional zone between the HCC and NEC tissues was also observed. The tumor was finally diagnosed as a combined-type primary mixed NEC–HCC tumor. After the diagnosis, the patient underwent somatostatin receptor scintigraphy to look for the primary NEC lesion, but no accumulations were found in any other part of her body. She has been free of recurrence for 6 months since the surgery.Conclusion: Mixed HCC–NEC tumors are very rare. Correct diagnosis requires multidisciplinary collaboration. Accumulating cases is needed to help understand their exact pathology, diagnosis and treatment.
Predictors of Mortality in COPD&nbsp;Exacerbation Cases Presenting to the Respiratory Intensive Care Unit
Authors: Cao Yang , Xing Zhenzhen , Long Huangyu , Huang Yilin , Guo Yanfei 
Published: October 2020

Abstract
Background: Studies report high in-hospital mortality of chronic obstructive pulmonary disease (COPD) exacerbations especially for those who requiring intensive care unit (ICU) admission. Recognizing factors associated with mortality in those patients could reduce healthcare costs and improve end-of-life care. Methods: This retrospective cohort study included 384 patients with AECOPD admitted to the respiratory ICU (RICU) of a tertiary hospital in Beijing from Jan 1, 2011 to Dec 31, 2018. Patients demographic characteristic, blood test results and comorbidities were extracted from the electronic medical record system and compared between survivors and non-survivors. Results: We finally enrolled 384 AECOPD patients, 44 (11.5%) patients died in hospital and 340 (88.5%) were discharged. The most common comorbidity was respiratory failure (294 (76.6%)), followed by hypertension (214 (55.7%)), coronary heart disease (CHD, 115 (29.9%)) and chronic heart failure (CHF, (76 (19.8%)). Multiple logistic regression analysis revealed the independent risk factors associated with in-hospital mortality included lymphocytopenia, leukopenia combined with CHF and the requirement for invasive mechanical ventilation (IMV).Conclusions: The in-hospital mortality of patients with COPD exacerbation requiring RICU admission is high. Lymphocytes<0.8×109/L, leukopenia, requirement for IMV, combined with CHF could be identified as risk factors associated with increased mortality rates.
“Breaking Down the Wall” Patients` and Families` Experience of Multifamily Therapy for Young Adult Women With Severe Eating Disorders
Authors: Brinchmann Berit Støre , Krvavac Sanja 
Published: October 2020

Abstract
Background: Eating disorders (EDs) are serious illnesses leading to lowered quality of life for the patients and their family. The Regional Centre for Eating disorders (RESSP) at Nordland Hospital in Bodø, Norway has developed an adjunct psychotherapeutic approach for the treatment of young adult patients with severe EDs. The patient's family members take part in the multifamily therapy (MFT) group programme. Methods: The aim of the study was to explore patients` and families` experience of MFT for young adult women with severe EDs. Data were collected by field observations in two MFT groups, qualitative group interviews and qualitative individual interviews with patients and their family members. The analytical method was qualitative content analysis. Results: Two main categories were identified: ‘Connectedness and recognition’ and ‘Open yourself and share’. MFT was described as a space for recognition within which it was possible to speak of things happening in the family with others with similar difficulties. It felt good and freeing, but also painful, to meet others with similar experiences. The participants shared a considerable loneliness because it is difficult for outsiders to grasp what it is like in a home dominated by an ED. The meeting with other families created an underlying safety. The participants received help to distinguish between realistic and unrealistic concerns and learned about openness and communication in relation to their daughter. Some women with EDs said that MFT was most important for the parents but also had been good for them as things had become better at home.Conclusion: The participants reported that their family had become better at talking to each other after having been in MFT. As a result, they were able to speak more openly about difficult things and share feelings. This gave rise to increased understanding. The study shows that MFT was found to be valuable and important. Never before had these families had such an offer, something so directly tailored to them. MFT for adults can be developed further and used in other groups, such as those concerning other chronic illnesses.
A Critical Narrative Inquiry to Understand the Impacts of an Overdose Prevention Site on the Lives of Site Users
Authors: Oudshoorn Abe , Bouck Michelle Sangster , McCann Melissa , Zendo Shamiram , Berman Helene , Banninga Jordan , Ber Marlene J Le , Zendo Zayya 
Published: October 2020

Abstract
Background Globally, communities are struggling to gain support for harm reduction strategies being implemented to address the impacts of substance use. A key part of this discussion is understanding and engaging with people who use drugs to help shape community harm reduction strategies. This study focused on how an overdose prevention site has influenced the lives of people who use drugs.MethodsA critical narrative method was utilized, centred on photo-narratives. Twenty-seven individuals accessing an overdose prevention site were recruited to participate in preliminary interviews. 16 participants subsequently took photographs to describe the impact of the site and participated in a second round of interviews. Through independent coding and several rounds of team analysis, four themes were proposed to constitute a core narrative encompassing the diverse experiences of participants. ResultsA key message shared by participants was the sense that their lives have improved since accessing the site. The core narrative proposed is presented in a series of four themes or ‘chapters’: Enduring, Accessing Safety, Connecting and Belonging, and Transforming. The chapters follow a series of transitions, revealing a journey that participants presented through their own eyes; one of moving from utter despair to hope, opportunity, and inclusion. Where at the outset participants were simply trying to survive the challenges of chaotic substance use, through the relationships and services provided at the site they moved towards small or large life transformations.ConclusionsThis study contributes to an enhanced understanding of how caring relationships with staff at the overdose prevention site impacted site users’ sense of self. We propose that caring relationships are an intervention in and of themselves, and that these relationships contribute to transformation that extends far beyond the public health outcomes of disease reduction. The caring relationships at the site can be a starting point for significant social changes. However, the micro-environment that existed within the site needs to extend beyond its walls for true transformative change to take place. The marginalization and stigmatization that people who use drugs experience outside these sites remains a constant barrier to achieving stability in their lives.