bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic analysis by RNA sequencing characterises malignant progression of canine insulinoma from normal tissue to metastatic disease
Authors: Capodanno Y. , Buishand F. O. , Pang L. Y. , Kirpensteijn J. , Mol J. A. , Elders R. , Argyle D. J. 
Published: July 2020

AbstractInsulinomas (INS) are the most common human and canine functioning pancreatic neuroendocrine tumours. The long-term prognosis for malignant INS is poor, because micrometastases are frequently missed during surgery. As human and canine malignant INS share clinical and histopathological features, dogs have been proposed as models for INS research. Using RNA-sequencing, we conducted a pilot study to better understand the underlying molecular mechanisms of canine INS. Normal canine pancreas and lymph node control tissues were compared with primary INS and INS-metastatic lymph nodes, revealing more than 3,000 genes differentially expressed in normal pancreas compared to primary INS. Only 164 genes were differentially expressed between primary INS and INS-metastatic lymph nodes. Hierarchical clustering analysis demonstrated similar genetic profiles in normal pancreas and early clinical stage primary INS, whereas late clinical stage primary INS resembled the genetic profile of INS-metastatic lymph nodes. These findings suggest that markers of malignant behaviour could be identified at the primary site of the disease. Finally, using the REACTOME pathways database, we revealed that an active collagen metabolism, extracellular matrix remodelling, beta-cell differentiation and non-beta-cell trans-differentiation might cause disease progression and hyperinsulinism in INS, identifying major pathways worthy of future research in this currently poorly controlled disease.
CT features of COVID-19 patients with two consecutive negative RT-PCR tests after treatment
Authors: Fu Zhao , Tang Ningning , Chen Yanqing , Ma Longbai , Wei Youyong , Lu Yumin , Ye Kun , Liu Hang , Tang Fen , Huang Guangyi , Yang Yingxia , Xu Fan 
Published: July 2020

AbstractThe objective of this study is to expound the CT features of COVID-19 patients whose throat swab samples were negative for two consecutive nucleic acid tests after treatment. We retrospectively reviewed 46 COVID-19 patients with two consecutive negative RT-PCR tests after treatment. The cases were divided into moderate group and severe/critical group according to disease severity. Clinical and CT scanning data were collected. CT signs of pulmonary lesions and the score of lung involvement were expounded. Thirty-nine moderate cases and seven severe/critical cases were included. Residual pulmonary lesions were visible in CT images. Moderate patients showed peripheral lesions while severe/critical cases exhibited both central and peripheral lesions with all lobes involvement. Mixed ground glass opacity (GGO) and pulmonary consolidation were noted. A larger proportion of severe patients showed reticular pulmonary interstitium thickening. Air bronchogram, pleural effusion, vascular enlargement, bronchial wall thickening, bronchiectasis, pleural thickening and pleural adhesion were more frequently observed in severe/critical group. The severe/critical group showed higher CT score. Pulmonary lesions persisted even after twice consecutive negative nucleic acid tests. We strongly recommended regular follow-up of CT scans after nucleic acid tests conversion. Evaluation of complete remission should base on chest CT.
Examination of the changes in lower extremities related to progression of adult spinal deformity: a longitudinal study of over 22 years
Authors: Shimizu Mutsuya , Kobayashi Tetsuya , Chiba Hisashi , Senoo Issei , Abe Satomi , Matsukura Keisuke , Ito Hiroshi 
Published: July 2020

AbstractThis longitudinal observational study investigated the relationship between changes in spinal sagittal alignment and changes in lower extremity coronal alignment. A total of 58 female volunteers who visited our institution at least twice during the 1992 to 1997 and 2015 to 2019 periods were investigated. We reviewed whole-spine radiographs and lower extremity radiographs and measured standard spinal sagittal parameters including pelvic incidence [PI], lumbar lordosis [LL], pelvic tilt [PT], sacral slope [SS] and sagittal vertical axis [SVA], and coronal lower extremity parameters including femorotibial angle (FTA), hip–knee–ankle angle (HKA), mechanical lateral distal femoral angle (mLDFA), mechanical medial proximal tibial angle (mMPTA) and mechanical lateral distal tibial angle (mLDTA). Lumbar spondylosis and knee osteoarthritis were assessed using the Kellgren–Lawrence (KL) grading system at baseline and at final follow-up. We investigated the correlation between changes in spinal sagittal alignment and lower extremity alignment and changes in lumbar spondylosis. The mean age [standard deviation (SD)] was 48.3 (6.3) years at first visit and 70.2 (6.3) years at final follow-up. There was a correlation between changes in PI-LL and FTA (R = 0.449, P < 0.001) and between PI-LL and HKA (R = 0.412, P = 0.001). There was a correlation between changes in lumbar spondylosis at L3/4 (R = 0.383, P = 0.004) and L4/5 (R = 0.333, P = 0.012) and the knee joints. Changes in lumbar spondylosis at L3/4 and L4/5 were related to changes in KOA. Successful management of ASD must include evaluation of the state of lower extremity alignment, not only in the sagittal phase, but also the coronal phase.
Higher tree diversity increases soil microbial resistance to drought
Authors: Gillespie Lauren M. , Fromin Nathalie , Milcu Alexandru , Buatois Bruno , Pontoizeau Clovis , Hättenschwiler Stephan 
Published: July 2020

AbstractPredicted increases in drought frequency and severity may change soil microbial functioning. Microbial resistance and recovery to drought depend on plant community characteristics, among other factors, yet how changes in plant diversity modify microbial drought responses is uncertain. Here, we assessed how repeated drying-rewetting cycles affect soil microbial functioning and whether tree species diversity modifies these effects with a microcosm experiment using soils from different European forests. Our results show that microbial aerobic respiration and denitrification decline under drought but are similar in single and mixed tree species forests. However, microbial communities from mixed forests resist drought better than those from mono-specific forests. This positive tree species mixture effect is robust across forests differing in environmental conditions and species composition. Our data show that mixed forests mitigate drought effects on soil microbial processes, suggesting greater stability of biogeochemical cycling in mixed forests should drought frequency increase in the future.
PDGFA gene rs9690350 polymorphism increases biliary atresia risk in Chinese children
Authors: Liu Fei , Zeng Jixiao , Zhu Deli , Xu Xiaogang , Lan Menglong , Wang Mengmeng , Zhao Jinglu , Xia Huimin , Zhang Yan , Zhang Ruizhong 
Published: July 2020

Abstract
Biliary atresia (BA) is a genetic and severe fibro-inflammatory obliterative cholangiopathy of neonates. Platelet-derived growth factor subunit A (PDGFA), as one of participants in liver fibrosis, the overexpression of PDGFA through DNA hypomethylation may lead to the development of BA, but the pathogenesis is still unclear. We conducted a large case–control cohort to investigate the association of genetic variants in PDGFA with BA susceptibility in the Southern Chinese population (506 cases and 1473 controls). We observed that the G allele of rs9690350(G&gt;C) in PDGFA was significantly associated with an increased risk of BA (OR = 1.24, 95% CI = 1.04–1.49, P=0.02). Additionally, the rs9690350 G allele increased the risk of non-cystic biliary atresia (OR = 1.26, 95% CI = 1.04–1.52, P=0.02) and was a genetic biomarker of severe manifestations after surgery. These findings indicate that the rs9690350 G allele is a PDGFA polymorphism associated with the risk of BA that may confer increased disease susceptibility.
Lack of association between IGF2BP2 rs4402960 polymorphism and gestational diabetes mellitus: a case–control study, meta-analysis and trial sequential analysis
Authors: Liu Jing , Song Guang , Zhao Ge , Meng Tao 
Published: July 2020

Abstract
Background: It is well known that insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) rs4402960 polymorphism is associated with Type 2 diabetes mellitus, which has a shared genetic background with gestational diabetes mellitus (GDM). Previous studies have yielded controversial results about the link between IGF2BP2 rs4402960 polymorphism and GDM risk. Thus, a meta-analysis was performed to obtain more conclusive results.
Methods: Clinical and genotype data were determined for 305 GDM and 1216 healthy participants recruited. Eligible studies were retrieved in PubMed, Web of science, EMBASE, and Scopus. Odds ratios (ORs) with 95% confidence intervals (CIs) were utilized to evaluate the relationship between IGF2BP2 polymorphisms and GDM susceptibility in five genetic models. The subgroup stratified analysis and trial sequential analysis (TSA) were performed.
Results: In this case–control study, no significant association was revealed between IGF2BP2 polymorphism and GDM (P&gt;0.05). When combined with the previous studies in the meta-analysis, there was no statistical association between IGF2BP2 polymorphism and GDM (allele model: OR = 1.01, 95% CI = 0.86–1.18; dominant model: OR = 1.00, 95% CI = 0.81–1.24; recessive model: OR = 1.08, 95% CI = 0.91–1.29; heterozygous model: OR = 0.99, 95% CI = 0.80–1.24; homozygous model: OR = 1.06, 95% CI = 0.78–1.42). No association was observed in five genetic models in each subgroup. TSA indicated sufficient proof of such null association in the overall population.
Conclusions: This meta-analysis provides sufficient statistical evidence indicating null association between IGF2BP2 rs4402960 polymorphism and GDM risk.
Bioinformatics-based analysis of the lncRNA–miRNA–mRNA and TF regulatory networks reveals functional genes in esophageal squamous cell carcinoma
Authors: Ye Zhimin , Fang Jun , Wang Zhun , Wang Lei , Li Bin , Liu Tongxin , Wang Yuezhen , Hua Jianfeng , Wang FangZheng , Fu Zhenfu 
Published: July 2020

AbstractEsophageal squamous cell carcinoma (ESCC) is a 5-year survival rate unsatisfied malignancies. The study aimed to identify the novel diagnostic and prognostic targets for ESCC. Expression profiling (GSE89102, GSE97051, and GSE59973) data were downloaded from the GEO database. Then, differentially expressed (DE) lncRNAs, DEmiRNAs, and genes (DEGs) with P-values &lt; 0.05, and |log2FC| ≥ 2, were identified using GEO2R. Functional enrichment analysis of miRNA-related mRNAs and lncRNA co-expressed mRNA was performed. LncRNA–miRNA–mRNA, protein–protein interaction of miRNA-related mRNAs and DEGs, co-expression, and transcription factors-hub genes network were constructed. The transcriptional data, the diagnostic and prognostic value of hub genes were estimated with ONCOMINE, receiver operating characteristic (ROC) analyses, and Kaplan–Meier plotter, respectively. Also, the expressions of hub genes were assessed through qPCR and Western blot assays. The CDK1, VEGFA, PRDM10, RUNX1, CDK6, HSP90AA1, MYC, EGR1, and SOX2 used as hub genes. And among them, PRDM10, RUNX1, and CDK6 predicted worse overall survival (OS) in ESCC patients. Our results showed that the hub genes were significantly up-regulated in ESCA primary tumor tissues and cell lines, and exhibited excellent diagnostic efficiency. These results suggest that the hub genes may server as potential targets for the diagnosis and treatment of ESCC.
What Does It Mean to Be Social? Defining the Social Landscape for Children With Childhood Apraxia of Speech
Authors: Tarshis Nancy , Winner Michelle Garcia , Crooke Pamela 
Published: July 2020

Purpose
What does it mean to be social? In addition, how is that different from behaving socially appropriately? The purpose of this clinical focus article is to tackle these two questions along with taking a deeper look into how communication challenges in childhood apraxia of speech impact social competencies for young children. Through the lens of early social development and social competency, this clinical focus article will explore how speech motor challenges can impact social development and what happens when young learners miss early opportunities to grow socially. While not the primary focus, the clinical focus article will touch upon lingering issues for individuals diagnosed with childhood apraxia of speech as they enter the school-aged years.

Conclusion
Finally, it will address some foundational aspects of intervention and offer ideas and suggestions for structuring therapy to address both speech and social goals.
Disseminierte kutane Sarkoidose mit nekrotisierenden Granulomen und erfolgreicher Dithranol-Therapie bei einem 75-jährigen Patienten
Authors: Darr-Foit S. , Schliemann S. , Elsner P. , Goetze S. 
Published: July 2020

ZusammenfassungBei der Sarkoidose handelt es sich um eine inflammatorische Erkrankung unbekannter Ätiologie, die sich hauptsächlich in der Lunge und in den thorakalen Lymphknoten manifestiert, die aber eine Vielzahl weiterer Organsysteme betreffen kann. Die Sarkoidose der Haut kann dabei mit oder ohne einer systemischen Beteiligung einhergehen und sich mit variablem klinischen Bild präsentieren. Die charakteristischen histologischen Befunde sind nicht-verkäsende („nackte“) Granulome ohne oder mit nur wenigen Lymphozyten oder Plasmazellen. In seltenen Fällen kann auch eine zentrale Nekrose beobachtet werden wie bei tuberkuloiden Granulomen.Topische und/oder systemische Kortikosteroide gelten als Goldstandard für die Behandlung der kutanen Sarkoidose. Dithranol ist in erster Linie als topisches Antipsoriatikum bekannt. Nach unserem Wissen ist über die Verwendung von Dithranol bei kutaner Sarkoidose bisher nicht in der Literatur berichtet worden. Es könnte sich dabei um eine wirksame Therapiealternative zu topischen Kortikosteroiden handeln. Wir berichten über einen Patienten, der unter Dithranol-Monotherapie erfolgreich behandelt wurde.
Rehabilitationssport und Funktionstraining als Vehikel für ein körperliches Training für Osteoporose-Betroffene – Grundlagen, Perspektiven und Limitationen
Authors: Beck Luisa , Sahar Jamil 
Published: July 2020

ZusammenfassungRehabilitationssport (RS) und Funktionstraining (FT) nach § 64 SGB IX bieten Menschen mit einer Osteoporose-Erkrankung die Möglichkeit, eigenverantwortlich Einfluss auf ihren gesundheitlichen Status zu nehmen. RS und FT sind nach ärztlicher Verordnung durch die Rehabilitationsträger zu bewilligen und werden in einer anerkannten Sportgruppe unter Leitung eines lizenzierten Übungsleiters durchgeführt. Der Leistungsumfang des RS und FT ist grundsätzlich nicht beschränkt. Beides kann bis zu 3-mal pro Woche verordnet werden, die Mindestdauer einer Übungseinheit (ÜE) liegt bei RS bei 45 min, bei FT bei 30 min bzw. 15 min. Die Teilnehmerobergrenze orthopädischer Indikationen liegt bei 15 Personen. RS- und FT-Gruppen sind zur Sicherung und Weiterentwicklung der Qualität der von ihnen erbrachten Leistung verpflichtet und müssen an Qualitätssicherungsprogrammen der Rehabilitationsträger teilnehmen. Betrachtet man neben den verbindlichen Regelungen die relativ hohe Dichte an Gruppen in Deutschland, so bietet sich der RS und FT als ideales Vehikel zur Sekundär- und Tertiärprävention der Osteoporose an. Allerdings ist die Effektivität von RS und FT auf indikationsspezifische Größen derzeit nicht nachgewiesen.
Factors Influencing Success Rate of Intracytoplasmic Sperm Injection with Azoospermic Male Patients
Authors: Rehnitz Julia , Rösner Sabine , Harsch Juliane , Dietrich Jens , Bruckner Thomas , Capp Edison , Strowitzki Thomas , Germeyer Ariane 
Published: July 2020

Abstract
Introduction Azoospermia affects about 1% of men, of whom up to 15% inquire about infertility treatment. Information about predictive factors for these couples is very limited.
Patients, Materials and Methods We performed a retrospective analysis of the clinical records of 118 cycles of intracytoplasmic sperm injection treatment after testicular sperm extraction for male azoospermia carried out between January 2008 and October 2015. Of those, 66 were first, 35 second, and 17 third cycles. Statistical significance was set at p < 0.05. Predictive factors for successful pregnancy were evaluated and included male/female age, male/female body mass index, male/female nicotine use, and histological results of testes biopsies.
Results Embryo quality and the number of embryos transferred were positively associated with pregnancy success (p = 0.003). Males whose partners conceived had a significantly lower body mass index than those whose partners did not conceive (p = 0.023). Neither female weight nor age nor smoking status of the male or female were significant factors. In cases with tubular atrophy ≥ SIGG grade 4 the chance of pregnancy was poor, irrespective of the existence of mature sperm and the number of cycles performed.
Conclusion Overweight male patients should be advised about weight reduction prior to treatment, and counseling about success rates should include histological and sperm-positive biopsy results.
Rare Ectopic Pregnancies – A Literature Review for the Period 2007 – 2019 on Locations Outside the Uterus and Fallopian Tubes
Authors: Eisner Sophie M. , Ebert Andreas D. , David Matthias 
Published: July 2020

AbstractThe majority of ectopic pregnancies (EP) are tubal pregnancies, but other implantation sites outside the uterus and tubes are also found. These rare EP locations present a particular diagnostic and therapeutic challenge. We present an overview of potential very rare locations of ectopic pregnancies, their symptoms, diagnosis and treatment, based on a systematic analysis of case reports. A literature review of the databases PubMed, Livivo and Google Scholar for the period 2007 to 2019 was carried out. A total of 113 publications were included in our review. These studies describe EP implantations in the posterior cul-de-sac, on the uterine serosa and uterine ligaments, in the vicinity of almost all intraperitoneal organs, on the abdominal wall as well as in retroperitoneal sites. The most common presenting symptom was abdominal pain occurring in different locations. The diagnostic procedures included various imaging procedures and/or explorative surgery at different
advanced stages of pregnancy. The most common and preferred option was laparotomy for surgical treatment. The placenta was successfully resected in the majority of cases. A rare EP location should be considered when making a differential diagnosis in patients of child-bearing age with abdominal pain.
Continuous Body Temperature Monitoring to Improve the Diagnosis of Female Infertility
Authors: Goeckenjan Maren , Schiwek Esther , Wimberger Pauline 
Published: July 2020

Abstract
Introduction Ovulatory dysfunction is a major cause of female infertility. We evaluated the use of continuous body temperature monitoring with a vaginal biosensor to improve
standard diagnostic procedures for determining ovulatory dysfunction.
Material and Methods This prospective interventional study was performed in a reproductive medicine department of a university hospital. The menstrual cycles of 51 women with
infertility were monitored and analysed using three different strategies: sonographic and hormonal assessment (standard approach), continuous core body temperature measurement and analysis
using the algorithm of OvulaRing, and lowest daily body temperature measurement monitored with a vaginal biosensor and analysed based on the body temperature curves used in natural family
planning.
Results Statistically significant differences were found in the temperature curves of women with luteal phase deficiency and polycystic ovary syndrome compared to women with normal
menstrual cycles. The analysis of individual cyclofertilograms can be used to detect cycle phases and estimate the date of ovulation.
Conclusions Continuous body temperature monitoring with a vaginal biosensor can improve the standard diagnostic procedures used to determine ovulatory dysfunction, especially if
dysfunction is due to luteal phase deficiency and polycystic ovary syndrome. Analysis of the lowest daily body temperature combined with the basal body temperature measurements used in
fertility awareness methods may be equieffective to continuous body temperature measurements with OvulaRing. The results of this study show that a revised diagnostic approach using fewer
hormonal assessments combined with continuous body temperature monitoring can reduce the number of appointments in an infertility clinic as well as the costs.
Aktuelle Definition und Diagnostik der pulmonalen Hypertonie
Authors: Lange T. J. , Borst M. , Ewert R. , Halank M. , Klose H. , Leuchte H. , Meyer F. J. , Seyfarth H.-J. , Skowasch D. , Wilkens H. , Held M. 
Published: July 2020

ZusammenfassungDie 6. Weltkonferenz für pulmonale Hypertonie (PH) fand vom 27. 2. – 1. 3. 2018 in Nizza statt. Hier wurden die Erkenntnisse auf dem Gebiet der PH der letzten 5 Jahre nach Aufarbeitung und Diskussion über einen längeren Vorbereitungszeitraum von 13 Arbeitsgruppen präsentiert und in der Folge zum Jahreswechsel 2018/2019 publiziert. Einer der seither intensiv diskutierten Vorschläge ist der einer Änderung der hämodynamischen Definition der PH mit Absenkung des Grenzwertes für den mittleren pulmonalarteriellen Druck von ≥ 25 auf > 20 mmHg, gemessen im Rechtsherzkatheter in Ruhe. Zusätzlich wurde der pulmonalvaskuläre Widerstand in die PH-Definition aufgenommen, was die Wichtigkeit der Bestimmung des Herzzeitvolumens während der Rechtsherzkatheteruntersuchung unterstreicht.Die Rationale sowie mögliche Auswirkungen der neuen PH-Definition, zu welchen zwischenzeitlich bereits neue Publikationen erschienen sind, möchten wir in diesem Übersichtsartikel diskutieren. Ferner ist ein aktueller Überblick zur nicht-invasiven und invasiven Diagnostik der PH enthalten, in welchem auf den Stellenwert der Methoden für Diagnostik, Differenzialdiagnostik und Prognose sowie weitere Neuerungen der 6.  PH-Weltkonferenz eingegangen wird. Ergänzt haben wir einen Abschnitt zum Stellenwert von Belastungsuntersuchungen für das Erkennen und die Verlaufsbeurteilung der PH, welche bei der Diskussion in Nizza und in den nachfolgenden Publikationen zumindest erwähnt, aber nicht ausführlicher besprochen wurden.
High grade dysplasia or esophageal adenocarcinoma in patients with a history of Roux-en-Y gastric bypass surgery: a case series
Authors: Alaber Omer , Mansoor Emad , Perez Lady Katherine Mejia , Dumot John , Bhatt Amit , Chak Amitabh 
Published: July 2020

Abstract
Background Roux-en-Y gastric bypass (RYGB) is the favored bariatric option in patients with gastroesophageal reflux and Barrett’s esophagus because it prevents reflux. Weight loss and decreased reflux following RYGB could theoretically minimize the risk of progression to cancer. We aimed to demonstrate the management of high grade dysplasia (HGD) and esophageal adenocarcinoma (EAC) developing in patients after RYGB.
Methods A prospectively maintained database was searched to identify cases of HGD and cancer in RYGB patients. Charts were reviewed for past history, endoscopic findings, endoscopic therapy, and pathology findings.
Results There were five cases where HGD/EAC developed several years after RYGB. The prior bariatric surgery precluded curative esophagectomy, illustrating the management challenges. All but one of the patients were uniquely and successfully managed with endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD).
Conclusions RYGB patients are still at risk of developing esophageal cancer. Patients at risk should be screened prior to RYGB and those with Barret’s esophagus need to undergo rigorous endoscopic surveillance following surgery. If detected early, EMR and ESD are invaluable in managing those who progress.