bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of cold atmospheric plasma on diabetes-induced enzyme glycation, oxidative stress, and inflammation; in vitro and in vivo
Authors: Rezaeinezhad Alireza , Eslami Pegah , Mirmiranpour Hossein , Ghomi Hamid 
Published: December 2019

AbstractCold atmospheric plasma (CAP) is known as the versatile tool in different biological, and medical applications. In this study, we investigated the effect of cold plasma on diabetes via in vitro and in vivo assessments. We performed the in vitro assay to evaluate the impact of CAP on glycated glutathione peroxidase (GPx) through enzyme activity measurement as a function index and far- and near-UV circular dichroism (CD) and fluorescence analysis as structure indices. The result of in vitro assessment showed that the exposure of glycated GPx to plasma causes a considerable increase in enzyme activity up to 30%. Also, the evaluation of far- and near-UV CD and fluorescence analysis indicated a modification in the protein structure. According to obtained result from in vitro assessment, in vivo assay evaluated the effect of CAP on diabetic mice through analyzing of blood glucose level (BGL), advanced glycation end products (AGEs), antioxidant activity, oxidative stress biomarkers such as malondialdehyde (MDA), advanced oxidation protein products (AOPP), and oxidized low-density lipoprotein (oxLDL), and inflammation factors including tumor necrosis factor (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6). The result of in vivo experiment also showed a 20% increase in antioxidant activity. Also, the reduction in AGEs, oxidative stress biomarkers, and inflammatory cytokines concentrations was observed. The result of this study revealed that CAP could be useful in diabetes treatment and can be utilized as a complementary method for diabetes therapy.
Surgical outcomes of endoscopic dacryocystorhinostomy: analysis of age effect
Authors: Sung Jae Yun , Lee Yeon Hee , Kim Kyoung Nam , Kang Tae Seen , Lee Sung Bok 
Published: December 2019

AbstractThere is limited evidence in literature determining age effect on outcomes of endoscopic dacryocystorhinostomy (EDCR) in adult patients with primary acquired nasolacrimal duct obstruction (NLDO). We aimed to analyze the outcomes of EDCR according to age in primary acquired NLDO. A retrospective study was performed on consecutive adult patients and patients were divided into two age groups; group 1 (aged to 61 years) and group 2 (aged 62 to 89 years) based on the average value. The minimum required follow-up period was 6 months. A total of 441 EDCRs performed in 342 patients were enrolled. The anatomical success rate was not significantly different between the two groups (91.8% and 88.2%, P = 0.209). However, the functional success rate was significantly lower in the group 2 (85.1% and 76.9%; P = 0.036). Functional failure was associated with old age and a history of diabetes mellitus (P  = 0.024 and P  = 0.008). In subgroup analysis of patients with anatomical success but functionally failed EDCR, group 2 had significantly more comorbid conditions such as eyelid laxity (P = 0.026). In conclusion, the comorbid conditions which increase with age may affect functional outcome, especially eyelid laxity, careful preoperative examination of the eyelid and conjunctiva should be emphasized to lacrimal surgeons before performing EDCR.
Tumor sidedness influences prognostic impact of lymph node metastasis in colon cancer patients undergoing curative surgery
Authors: Lai Hsin-Wu , Wei James Cheng-Chung , Hung Hung-Chang , Lin Chun-Che 
Published: December 2019

AbstractThis study aimed to evaluate prognostic impacts of the number of lymph nodes (LNs) examined and LN ratio on cancer-specific mortality after surgery in patients with right-sided colon cancer (RCC) or left-sided colon cancer (LCC) using the Surveillance, Epidemiology, and End Results database. Number of LNs examined and LN ratio were treated as categorical and/or continuous. Competing risks proportional hazards regressions adjusted by propensity score were performed. All included patients had stage I, II, or III disease, and 45.1% of them had RCC. RCC and LCC patients with high level of LNs examined had better prognosis after segmental resection or hemicolectomy. RCC and LCC patients with higher LN ratio had worse prognosis regardless of surgery. Survival benefit of having high level of LNs examined was observed in RCC patients with stage I, II, or III disease, but only in LCC patients with stage II disease. Both higher LN ratio and high level of LN were negative prognostic factors for cancer-specific mortality in stage III patients regardless of tumor sidedness. In conclusion, RCC patients in various conditions had worse or comparable prognosis compared to their LCC counterparts, which reflected the severity of LN metastasis.
Interannual to decadal variability within and across the major Eastern Boundary Upwelling Systems
Authors: Bonino Giulia , Di Lorenzo Emanuele , Masina Simona , Iovino Doroteaciro 
Published: December 2019

AbstractClimate variability and climate change in Eastern Boundary Upwelling Systems (EBUS) affect global marine ecosystems services. We use passive tracers in a global ocean model hindcast at eddy-permitting resolution to diagnose EBUS low-frequency variability over 1958–2015 period. The results highlight the uniqueness of each EBUS in terms of drivers and climate variability. The wind forcing and the thermocline depth, which are potentially competitive or complementary upwelling drivers under climate change, control EBUS low-frequency variability with different contributions. Moreover, Atlantic and Pacific upwelling systems are independent. In the Pacific, the only coherent variability between California and Humboldt Systems is associated with El Niño Southern Oscillation. The remaining low-frequency variance is partially explained by the North and South Pacific expressions of the Meridional Modes. In the Atlantic, coherent variability between Canary and Benguela Systems is associated with upwelling trends, which are not dynamically linked and represent different processes. In the Canary, a negative upwelling trend is connected to the Atlantic Multi-decadal Oscillation, while in the Benguela, a positive upwelling trend is forced by a global sea level pressure trend, which is consistent with the climate response to anthropogenic forcing. The residual variability is forced by localized offshore high sea level pressure variability.
Human β-defensin-3 reduces excessive autophagy in intestinal epithelial cells and in experimental necrotizing enterocolitis
Authors: Chen Liping , Lv Zhibao , Gao Zhimei , Ge Guijie , Wang Xueli , Zhou Junmei , Sheng Qingfeng 
Published: December 2019

AbstractNecrotizing enterocolitis (NEC) is a leading cause of mortality in preterm newborns. Intestinal barrier dysfunction is one key event in NEC pathogenesis. Human β-defensin-3 (hBD3), one member of cationic host defence peptides, was reported to reduce the development of necrotizing enterocolitis in a neonatal rat model. And autophagy was induced in the intestine of human and animals with NEC. We hypothesized that regulation of autophagy might play a critical role in hBD3-mediated protection against NEC injury. Autophagy activity was evaluated both in intestinal epithelial cells and in NEC models. Newborn Sprague-Dawley rats were divided randomly into four groups: Control + NS, Control + rapamycin, NEC + NS, and NEC + hBD3. Body weight, histological score, survival time, enterocyte migration and mucosal barrier were recorded. Our results showed that hBD3 pretreatment could effectively inhibit autophagy activity in cultured IEC-6 and Caco2 enterocytes, and CXCR4 might be involved in hBD3-mediated autophagy suppression. Moreover, hBD3-induced inhibition of autophagy significantly promoted the intestinal epithelial cell migration by wound healing assay and transwell migration assay. In the rat model of NEC, hBD3 could noticeably reduce the expression of autophagy-activated proteins, down-regulate the expression of inflammatory mediators, and promote the mucosal integrity. Our data suggest an additional role of hBD3-mediated protection against intestinal mucosal injury: inhibition of over-activated autophagy in enterocytes.
The high risk for type 2 diabetes among ethnic minority populations is not explained by low-grade inflammation
Authors: Muilwijk Mirthe , Nieuwdorp Max , Snijder Marieke B. , Hof Michel H. P. , Stronks Karien , van Valkengoed Irene G. M. 
Published: December 2019

AbstractOur aim was to identify whether low-grade inflammation, reflected by C-reactive protein (CRP), explains the higher risk for incident type 2 diabetes (T2D) among ethnic minorities. We included 837 Dutch, 712 South-Asian Surinamese, 797 African Surinamese, 804 Ghanaian, 817 Turkish and 778 Moroccan origin participants of the HELIUS study (Amsterdam, the Netherlands). We used multiple linear regression to assess ethnic differences in CRP levels. We determined the association of CRP with T2D and the modifying effect of ethnicity by cox regression, and compared hazard ratios for the association between ethnicity and T2D before and after adjustment for CRP. CRP levels were higher in ethnic minority groups than in Dutch origin participants. CRP was associated with a higher T2D incidence, similarly across ethnic groups (overall HR per SD 1.38 [95% CI 1.14; 1.68]). However, the association was attenuated and no longer statistically significant after adjustment for adiposity measures (HR 1.11 [95% CI 0.90; 1.37]). CRP accounted for a very small part of the ethnic differences in T2D, but only in models unadjusted for adiposity. Low-grade inflammation does not substantially contribute to the higher risk of T2D among ethnic minority populations compared to the Dutch.
Dichotomic effects of clinically used drugs on tumor growth, bone remodeling and pain management
Authors: Barrière David André , Midavaine Élora , Doré-Savard Louis , Kirby Karyn , Tremblay Luc , Beaudoin Jean-François , Beaudet Nicolas , Longpré Jean-Michel , Lecomte Roger , Lepage Martin , Sarret Philippe 
Published: December 2019

AbstractImprovements in the survival of breast cancer patients have led to the emergence of bone health and pain management as key aspects of patient’s quality of life. Here, we used a female rat MRMT-1 model of breast cancer-induced bone pain to compare the effects of three drugs used clinically morphine, nabilone and zoledronate on tumor progression, bone remodeling and pain relief. We found that chronic morphine reduced the mechanical hypersensitivity induced by the proliferation of the luminal B aggressive breast cancer cells in the tumor-bearing femur and prevented spinal neuronal and astrocyte activation. Using MTT cell viability assay and MRI coupled to 18FDG PET imaging followed by ex vivo 3D µCT, we further demonstrated that morphine did not directly exert tumor growth promoting or inhibiting effects on MRMT-1 cancer cells but induced detrimental effects on bone healing by disturbing the balance between bone formation and breakdown. In sharp contrast, both the FDA-approved bisphosphonate zoledronate and the synthetic cannabinoid nabilone prescribed as antiemetics to patients receiving chemotherapy were effective in limiting the osteolytic bone destruction, thus preserving the bone architecture. The protective effect of nabilone on bone metabolism was further accompanied by a direct inhibition of tumor growth. As opposed to zoledronate, nabilone was however not able to manage bone tumor-induced pain and reactive gliosis. Altogether, our results revealed that morphine, nabilone and zoledronate exert disparate effects on tumor growth, bone metabolism and pain control. These findings also support the use of nabilone as an adjuvant therapy for bone metastases.
Age-related positivity effect on behavioural responses of dogs to human vocalisations
Authors: Smit Iris , Szabo Dora , Kubinyi Enikő 
Published: December 2019

AbstractAge-related changes in the brain can alter how emotions are processed. In humans, valence specific changes in attention and memory were reported with increasing age, i.e. older people are less attentive toward and experience fewer negative emotions, while processing of positive emotions remains intact. Little is yet known about this “positivity effect” in non-human animals. We tested young (n = 21, 1–5 years) and old (n = 19, >10 years) family dogs with positive (laugh), negative (cry), and neutral (hiccup, cough) human vocalisations and investigated age-related differences in their behavioural reactions. Only dogs with intact hearing were analysed and the selected sound samples were balanced regarding mean and fundamental frequencies between valence categories. Compared to young dogs, old individuals reacted slower only to the negative sounds and there was no significant difference in the duration of the reactions between groups. The selective response of the aged dogs to the sound stimuli suggests that the results cannot be explained by general cognitive and/or perceptual decline. and supports the presence of an age-related positivity effect in dogs, too. Similarities in emotional processing between humans and dogs may imply analogous changes in subcortical emotional processing in the canine brain during ageing.
Genome-scale CRISPR activation screen uncovers tumor-intrinsic modulators of CD3 bispecific antibody efficacy
Authors: Decker Corinne E. , Young Tara , Pasnikowski Elizabeth , Chiu Joyce , Song Hang , Wei Yi , Thurston Gavin , Daly Christopher 
Published: December 2019

AbstractBispecific antibodies (bsAb) that bridge tumor cells and CD3-positive effector T cells are being developed against many tumor cell targets. While tumor cell factors other than target expression level appear to play a role in determining the efficacy of CD3 bsAb, the identity of such factors remains largely unknown. Using a co-culture system of primary human T cells and B lymphoma cell lines, we demonstrate a range of sensitivities to CD20xCD3 bsAb that is independent of CD20 surface expression. To identify genes that modulate tumor cell sensitivity to CD3 bsAb, we employed a genome-scale CRISPR activation screen in a CD20xCD3-sensitive human B lymphoma cell line. Among the most highly enriched sgRNAs were those targeting genes with predicted effects on cell-cell adhesion, including sialophorin (SPN). Increased expression of SPN impeded tumor cell clustering with T cells, thereby limiting CD3 bsAb-mediated tumor cell lysis. This inhibitory effect of SPN appeared to be dependent on sialylated core 2 O-glycosylation of the protein. While SPN is not endogenously expressed in the majority of B cell lymphomas, it is highly expressed in acute myeloid leukemia. CRISPR-mediated SPN knockout in AML cell lines facilitated T cell-tumor cell clustering and enhanced CD3 bsAb-mediated AML cell lysis. In sum, our data establish that the cell cross-linking mechanism of CD3 bsAb is susceptible to subversion by anti-adhesive molecules expressed on the tumor cell surface. Further evaluation of anti-adhesive pathways may provide novel biomarkers of clinical response and enable the development of effective combination regimens for this promising therapeutic class.
Association between constitution, medical history, axiography and postural control in women aged between 21 to 30 years
Authors: Doerry C. , Fisch V. , Schamberger S. , Kopp S. , Erbe C. , Wanke E. M. , Groneberg D. A. , Ohlendorf D. 
Published: December 2019

AbstractThe aim of this study was to determine association between constitutional, medical history and axiographic parameters with postural control parameters. Overall, 106 healthy female subjects aged between 21 and 30 years were measured. Data collection was carried out by completing a questionnaire on constitutional parameters, illnesses, accidents and medical/orthodontic therapies, as well as by axio- and posturographic measurements. Data were analyzed using correlations, pair comparisons and group comparisons. The significance level was set at p ≤ 0.05. The statistical evaluation showed significant correlations between sporting exercise and body sway in the sagittal direction (p ≤ 0.03), the BMI and the load on the forefoot/rear foot (p ≤ 0.01), the mouth opening and the load on the forefoot/rearfoot (p ≤ 0.01) and the presence of a deviation with the load on the left/right foot (p ≤ 0.01). The physical condition as well as the temporo-mandibular system are associated with the postural control in young women. Therefore, a holistic diagnosis and therapy will be supported by the present outcomes.
Soil mite communities (Acari: Mesostigmata, Oribatida) as bioindicators for environmental conditions from polluted soils
Authors: Manu Minodora , Honciuc Viorica , Neagoe Aurora , Băncilă Raluca Ioana , Iordache Virgil , Onete Marilena 
Published: December 2019

AbstractAn anthropic ecosystem from Romania was investigated from acarological, vegetation and chemical point of view. The community structures of two groups of mites were studied (Acari: Mesostigmata, Oribatida) from a tailing pond, using transect method, in correlation with concentrations of heavy metals (As, Cu, Pb, Ni, Mn and Zn), with abiotic factors (altitude, aspect, soil temperature, soil humidity, soil pH) and biotic factor (vegetation coverage). Taking into account the mite communities, in total, 30 mite species were identified, with 1009 individuals and 18 immatures (10 species with 59 individuals, 5 immatures of Mesostigmata and 20 species with 950 individuals, 13 immatures of Oribatida). The investigated habitats from the tailing pond were grouped in five transects, with different degree of pollution, based on total metal loads. Taking into account of the connection between mites communities, abiotic factors and heavy metals, each transect were characterized through specific relationship. Using multivariate statistical analysis, we revealed that the occurrence of some Oribatida species was strongly correlated with vegetation coverage, soil pH and soil humidity, though concentrations of Cu, As, Mn, Ni and Zn also had an influence. Pb and Zn concentrations were shown to influence the occurrence of Mesostigmata mites. The heterogeneity of mites species richness at 2 m2scale was correlated with a metric related to the heterogeneity of heavy metals at the same scale.
Characterization of a Novel Simian Sapelovirus Isolated from a Cynomolgus Monkey using PLC/PRF/5 Cells
Authors: Zhang Wenjing , Kataoka Michiyo , Doan Hai Yen , Ami Yasushi , Suzaki Yuriko , Takeda Naokazu , Muramatsu Masamichi , Li Tian-Cheng 
Published: December 2019

AbstractWe isolated a novel simian sapelovirus (SSV), Cam13, from fecal specimen of a cynomolgus monkey by using PLC/PRF/5 cells. The SSV infection of the cells induced an extensive cytopathic effect. Two types of virus particles with identical diameter (~32 nm) but different densities (1.348 g/cm3 and 1.295 g/cm3) were observed in the cell culture supernatants. The RNA genome of Cam13 possesses 8,155 nucleotides and a poly(A) tail, and it has a typical sapelovirus genome organization consisting of a 5’ terminal untranslated region, a large open reading frame (ORF), and a 3’ terminal untranslated region. The ORF encodes a single polyprotein that is subsequently processed into a leader protein (L), four structural proteins (VP1, VP2, VP3, and VP4) and seven functional proteins (2A, 2B, 2C, 3A, 3B, 3C, and 3D). We confirmed that 293 T, HepG2/C3A, Hep2C, Huh7 and primary cynomolgus monkey kidney cells were susceptible to SSV infection. In contrast, PK-15, Vero, Vero E6, RD-A, A549, and primary green monkey kidney cells were not susceptible to SSV infection. We established an ELISA for the detection of IgG antibodies against SSV by using the virus particles as the antigen. A total of 327 serum samples from cynomolgus monkeys and 61 serum samples from Japanese monkeys were examined, and the positive rates were 88.4% and 18%, respectively. These results demonstrated that SSV infection occurred frequently in the monkeys. Since Cam13 shared 76.54%–79.52% nucleotide sequence identities with other known SSVs, and constellated in a separate lineage in the phylogeny based on the entire genome sequence, we propose that Cam13 is a new genotype of the simian sapelovirus species.
Efficient large amplitude primary resonance in in-extensional nanocapacitors: Nonlinear mean curvature component
Authors: Rahmanian Sasan , Hosseini-Hashemi Shahrokh , SoltanRezaee Masoud 
Published: December 2019

AbstractIn general, the impact of geometric nonlinearity, which arises from geometric relationships governing the motion of constituent particles of elastic mediums, becomes critically important while the system operates under large deformations. In this case, the influence of different physics governing the system dynamics might be coupled with the impact of geometric nonlinearity. Here, for the first time, the non-zero component of the mean curvature tensor is nonlinearly expressed in terms of the middle-axis curvature of a cantilevered beam. To this aim, the concept of local displacement field together with inextensibility condition are employed. A nanowire-based capacitor is assumed to be excited by the electrostatic load that is composed of both DC and AC voltages. The main concern is on the case, in which it is necessary to polarize the electrodes with large amplitude voltages. Other physics, including surface strain energy, size-dependency, and dispersion force are modeled to predict the system response more accurately. Hamilton’s principle is used to establish the motion equation, and the Galerkin method is applied to exploit a set of nonlinear ordinary differential equations (ODEs). Implementing a combination of shooting and arc-length continuation scheme, the frequency and force-displacement behaviors of the capacitor are captured near its primary resonance. The coupled effects of the nonlinear impact factor, surface elasticity and size parameters on the bifurcation point’s loci and dynamic pull-in instability are studied.
Transdermal delivery of nobiletin using ionic liquids
Authors: Hattori Tadashi , Tagawa Hiroki , Inai Makoto , Kan Toshiyuki , Kimura Shin-ichiro , Itai Shigeru , Mitragotri Samir , Iwao Yasunori 
Published: December 2019

AbstractNobiletin (NOB), a flavonoid, has extremely low water solubility and low oral bioavailability; however, despite these problems, various physiological effects have been investigated in vitro. In the present study, we investigated the transdermal delivery of NOB using choline and geranic acid (CAGE), which is a biocompatible material that has been reported to be a promising transdermal delivery approach. The feasibility was evaluated by a set of in vitro and in vivo tests. A solubility evaluation demonstrated that CAGE induced excellent solubility of NOB induced by multipoint hydrogen bonding between NOB and CAGE. In vitro transdermal tests using a Franz diffusion cell showed that CAGE was effective in enhancing transdermal absorption of NOB, compared to other penetration enhancers. Subsequent in vivo tests demonstrated that CAGE significantly improved area under the concentration-time curve of NOB in vivo and NOB/CAGE sample showed 20-times higher bioavailability than oral administration of NOB crystal. Furthermore, NOB/CAGE sample also showed significant drops of the blood glucose level in rats derived from hypoglycemic activity of NOB. Thus, transdermal administration of NOB using CAGE was shown to be feasible, which indicates that the use of CAGE may be adapted for other flavonoids that also show both low water solubility and low permeability.