bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Mastery Of Cognitive, Affective, And Psychomotor Domains On The Students’ Decision To Be Entrepreneurs In Facing Towards Asean Economic Community-2015
Authors: Amaliana Lutfatul 
Published: February 2020

Abstract
The purpose of this study was to analyze the depth of student understanding in the cognitive, affective, cognitive, and psychomotor domains of Entrepreneurship subjects by using a contextual learning approach to the decision to become entrepreneurs. This research used a descriptive research method with qualitative and quantitative approaches. Descriptive analysis was conducted because this research only gave an overview of the learning conditions in the classroom during the learning process and analyzes the learning outcomes in Entrepreneurship subject by using Contextual Teaching and Learning. Contextual learning (CTL) can be used to increase student participation in discussions, the results of the depth of students' understanding of cognitive, affective, and psychomotor domains have a positive effect on the decision to become entrepreneurs. No previous research comprehensively investigated the domains of cognitive, affective, and psychomotor in the decisions of students to become entrepreneurs in the face of the ASEAN-2015 economic community. This is not only to reduce the rising unemployment rate, but also to face the inevitable changes in the social and economic conditions of the world.
Health System Strategies and Adolescent Sexual Health: Systematic review using Rodgers Concept Analysis Framework
Authors: Nunu Wilfred Njabulo , Makhado Lufuno , Mabunda Jabu Tsakani , Lebese Rachel Tsakani 
Published: February 2020

Abstract
Background: Health Systems Strategies have been key in determining Adolescent Sexual Health outcomes. This study therefore aims to review literature on relationship between Health Systems Strategies and Adolescent Sexual Health issues guided by Rodgers evolutionary concept analysis framework. The study further develops a Conceptual Framework that would guide a study that seeks to “develop strategies to facilitate safe sexual practices in adolescents through Integrated Health Systems in selected Districts in Zimbabwe”.Methods: The words Adolescents, Health Systems, Sexual Health and Strategies were used to search for published literature (in English) on Google Scholar, PUBMED, EBSCO and Science Direct. 111 Articles and 04 reports were obtained and their content screened for relevance and 32 articles 01 report were found suitable and relevant and thus were reviewed. Thematic analysis to identify attributes, antecedents and consequences of Health Systems Strategies on Adolescent Sexual Health was done. These findings were then used to inform the development of the Conceptual Framework.Results: Identified attributes were: contextual, dynamic, engaging and advocating, and inefficient Health Systems in addressing Adolescent Sexual Health. Identified antecedents of these Health System Strategies on Adolescent Sexual Health included; adolescent sexual rights, lack of understanding of what sexual health is, need for integrated Adolescent Sexual Health systems, available resources and type of society. Consequences of these Health Systems Strategies on Adolescent Sexual Health were identified to be; weak Adolescent Sexual Health programs, parallel and fragmented Health Systems, vulnerability and heightened risks for poor health outcomes and challenges in interacting with different ethnic groups and gender.Conclusions: Adolescents are very vulnerable and need to be protected at all costs. There is need to have comprehensive Health Systems Strategies that would positively influence Adolescent Sexual Health.
The preliminary investigation of potential response biomarkers to air pollution (PAHs) exposure on childhood asthma
Authors: Bao Yuling , Jin Rui , Hu Jinye , Sun Zhe , Xue Mingyan , Bian Zilong , Zhan Zhang , Chen Feng , Li Lei , Wu qian 
Published: February 2020

Abstract
Background In recent five years, air quality in China has been gradually improved; however, the prevalence of asthma in China has still increased significantly. This study aims to examine whether air pollution exposure is a significant contributor to childhood asthma and to explore the mechanisms using exposure and response biomarkers. Methods The generalized additive model was applied to evaluate the effects of air pollutants on asthma hospitalization. Urinary 1-hydroxypyrene (1-OHPyr) was determined as PAHs exposure biomarker. Metabolic profiles and microbial diversity were screened and selected as response biomarkers. Results Daily average concentrations of the six common air pollutants were not significantly associated with daily childhood asthma hospitalization. Urinary 1-OHPyr level showed large variability among asthmatic children and were positively correlated with the abundance of Prevotella (genus level) and purine metabolism. Conclusions The biomarker of PAHs exposure is associated with metabolic and microbiological biomarkers of childhood asthma. As the potential health risk, we suggest that PAHs should be included in the national monitoring system aiming to reduce the exposure.
Influence Of Time Under Mechanical Ventilation On Bronchopulmonary Dysplasia Severity In Extremely Preterm Infants: A Pilot Study.
Authors: Probst Vanessa Suziane , Escobar Victoria , Soares Darllyana , Kreling Jane , Ferrari Ligia , Felcar Josiane M. , Camillo Carlos A. M. 
Published: February 2020

Abstract
The relation between mechanical ventilation (MV) and bronchopulmonary dysplasia (BPD) is well stabilished, but is unknown, however, how much time under MV influences the severity of the disease. Aim: To define the duration under MV with greater chance to develop moderate to severe BPD (M/S BPD) in extremely PTNB and to compare clinical outcomes before and during hospitalization among patients with mild and M/S BPD. Methods: 53 PTNB were separated into mild and M/S BPD groups and their data were analyzed. Time under MV with a greater chance of developing M/S BPD was estimated by the ROC curve. Perinatal and hospitalization outcomes were compared between groups. A logistic regression was performed to verify the influence of variables associated to M/S BPD development, such as pulmonary hypertension (PH), gender, gestational age (GA) and weight at birth, as well the time under MV found with ROC curve. The result of ROC curve was validated using an independent sample (n=16) by Chi-square test. Results: Time under MV related to a greater chance of developing M/S BPD was 36 days. M/S BPD group had more males (14 vs 5, p=0,047), longer time under MV (43 vs 19 days, p<0,001), more individuals with PH (12 vs 3, p=0,016), worse retinopathy of prematurity (grade 3, 2 vs 11, p=0,003), longer hospital length of stay (109 vs 81,5 days, p<0,001), greater PMA (41 vs 38 weeks, p<0,001) and weight (2620 vs 2031 grams, p<0,001) at discharge and the mild BPD group had more CPAP use prior to MV (12 vs 7, p=0,043). Among all variables included in logistic regression, only PH and MV<36 days were significant in the model, explaining 72% of variation in M/S BPD development. In the validation sample, prevalence of preterm infants who needed MV for more than 36 days in the M/S BPD group was 100% (n=6) and 0% in mild BPD group (p=0,0001). Conclusion: Time under MV related to moderate to severe BPD development is 36 days, and worst outcomes are related to disease severity. PH and time under MV for more than 36 days are related to development of M/S BPD.
A comparative analysis of erythropoietin and carbamoylated erythropoietin-induced proteome profiles
Authors: Tiwari Neeraj Kumar , Sathyanesan Monica , Kumar Vikas , Newton Samuel S 
Published: February 2020

Abstract
In recent years erythropoietin (EPO) has emerged as a useful neuroprotective and neurotrophic molecule that produces antidepressant and cognitive enhancing effects in psychiatric disorders. However, EPO robustly induces erythropoiesis and elevates red blood cell counts. Chronic administration is therefore likely to increase blood viscosity and produce adverse effects in non-anemic populations. Carbamyolated erythropoietin (CEPO), a chemically engineered modification of EPO, is non-erythropoietic but retains the neurotrophic and neurotrophic activity of EPO. Blood profile analysis after EPO and CEPO administration showed that CEPO has no effect on red blood cell or platelet counts. We conducted an unbiased, quantitative, mass spectrometry-based proteomics study to comparatively investigate EPO and CEPO-induced protein profiles in neuronal phenotype PC12 cells. Bioinformatics enrichment analysis of the protein expression profiles revealed the upregulation of protein functions related to memory formation such as synaptic plasticity, long term potentiation (LTP), neurotransmitter transport, synaptic vesicle priming, and dendritic spine development. The regulated proteins, with roles in LTP and synaptic plasticity, include Neudesin, Chromogranin b, Cortactin, Elongation initiation factor 3a and Proteasome 26s subunit, ATPase. We examined the expression of a subset of regulated proteins by immunohistochemical analysis in mouse brain. The results of our study sheds light on potential mechanisms whereby EPO and CEPO produce cognitive enhancing effects in clinical and preclinical studies.
Increased Energy Expenditure and Energy Loss through Feces Contribute to the Long-term Outcome of Roux-en-Y Gastric Bypass on Diet Induced Obese Mouse Model
Authors: Chen Kai , Xiao Boen , Zhou Zhe , Peng Weihui , liu wei 
Published: February 2020

Abstract
Background Roux-en-Y gastric bypass (RYGB) has been proved to be more effective than other bariatric procedures in long-term on body weight loss and remission of diabetes. However, the mechanism remains poorly understood. Long-term change of energy metabolism after RYGB has rarely been reported. Objectives To investigate the long-term outcome of RYGB on mouse model and its mechanism from the perspective of energy metabolism. Methods High-fat diet induced obesity (DIO) mice were assigned to two groups receiving RYGB(n=8) and sham operation(n=7), followed by high-fat diet feeding until 12 weeks after surgery. Body weight and food intake were recorded weekly, measurement of body composition and energy metabolism by metabolic chamber were conducted on week 4, 8 and 12 after surgery. Fecal energy measurement, Intraperitoneal Glucose Tolerance Test (IPGTT) and Insulin Tolerance Test (ITT) were conducted on week 12 after surgery.Results Food intake was reduced in RYGB group within the first 3 weeks after surgery and increased to be the same withSham group from postoperative week 4. At 12 weeks after surgery, body weight reduced by 36±3.2% in RYGB group comparing to 16±2% body weight gain in Sham group, while fat mass was significantly reduced in RYGB group than in Sham group (9.2±1.5% versus 30.1±0.7%). Energy expenditure was significantly higher on postoperative week 8 in RYGB group than in Sham group. In comparison with Sham group, respiratory exchange ratio (RER) was unchanged, decreased and increased in RYGB group at postoperative week 4, 8 and 12, respectively. Fecal energy measurementshowed that feces from mice in RYGB group contained higher energy level than Sham group. Glucose metabolism was significantly improved in RYGB group in contrast to Sham group, demonstrated by the result of Intraperitoneal Glucose Tolerance Test (IPGTT) (AUC: 1502± 104 versus 2277±198, respectively) and the Insulin Tolerance Test (ITT) (AUC: 524 ±50 versus 838±63, respectively). Conclusions Increased energy expenditure and energy loss through feces contribute to the long-term body weight control after RYGB. Enhanced glucose utilization might play a role in the long-term improvement of glucose metabolism.
Effect of monoacylglycerol lipase inhibition on intestinal permeability in chronic stress model
Authors: Wang Jing , Zhang Xiaohua , Yang Chongmei , Zhao Shulei 
Published: February 2020

Abstract
Background: The endocannabinoid 2-arachidonoylglycerol (2-AG) is an anti-nociceptive lipid, which is inactivated through cellular uptake and subsequent catabolism by monoacylglycerol lipase (MAGL). The present study aimed to explore the effects of inhibition of MAGL on intestinal permeability. Methods: We first tested it in differentiated CaCO2 cells after 21 days’ culture. The rat model of water avoidance stress (WAS) was established, and rats were divided into four groups according to intervention. Rats received intraperitoneal injection (i.p.) of an MAGL inhibitor (JZL184) alone, JZL184 and the cannabinoid receptor 1 (CB1) antagonist (SR141716A), JZL184 and a cannabinoid receptor 2 (CB2) antagonist (AM630) or vehicle alone (control). We analyzed the fluorescein isothiocyanate-dextran (FD4) permeability and 2-AG level. Expression of MAGL and tight-junction-associated proteins were detected by western blot. Results: Compared with the control group, MAGL expression was higher and 2-AG levels lower among WAS rats. Intestinal permeability was increased following administration of JZL184 which occurred due to up-regulation of tight-junction-associated proteins Claudin-1, Claudin-2, Claudin-5 and Occludin.Conclusion: The effects of MAGL inhibition were mediated by CB1, indicating that MAGL may represent a novel target for the treatment of reduced intestinal permeability in the context of chronic stress.
The Association between Gut Microbiota and Intestinal Toll-like Receptors Genes Expression in Adenomatous and Colorectal Cancerous patients
Authors: Rezasoltani Sama , Sharafkhah Maryam , Aghdaei Hamid Asadzadeh , Olfatifar Meysam , Mojarad Ehsan Nazemalhosseini , Yadegar Abbas , Zali Mohammad Reza 
Published: February 2020

Abstract
Background: Toll-like receptor (TLR) signaling has been implicated in colorectal cancer (CRC) development. Intestinal microbiota can affect the expression of TLRs, which may induce inflammatory responses and impair the gut homeostasis. Here, we aimed to evaluate certain intestinal microbiota related to TLRs expression in colonic tissues of adenomatous polyposis and CRC patients. Results: Fecal and colonic tissue samples were collected from normal controls (NC), adenomatous (AP) cases and (CRC) patients via colonoscopy for CRC screening during 2016 to 2018. Fecal samples were collected to analyze intestinal bacteria including Streptococcus bovis , Enterococcus faecalis , Bacteroides fragilis , enterotoxigenic Bacteroides fragilis (ETBF) , Fusobacterium nucleatum , Porphyromonas gingivalis, Porphyromonas spp . and Roseburia spp . by real-time PCR. Gene expression of TLR2, TLR4 and TLR5 was examined in colonic tissues by qRT-PCR. Different abundant of gut bacteria were achieved in NC, AP and CRC groups. The genes expression of TLR2, TLR4 and TLR5 were significantly different in AP and CRC cases vs. normal group (P value <0.05). There was a significant relationship between TLR2, TLR4, TLR5 genes expression and Roseburia spp., P. gingivalis and ETBF quantity in normal group. Also significant association between TLR2, TLR4 genes expression levels and the quantity of S.bovis , ETBF, Roseburia spp. and E. faecalis in AP and CRC cases were achieved. Conclusion : Intestinal expression of TLR2, TLR4 and TLR5 is dynamic and depends on gut microbiota. Hence, altered immune activation in response to dysbiotic microbiota may promote intestinal inflammation in a group of patients with AP and CRC. Keyword: Adenomatous polyposis; colorectal cancer; gut microbiota; Toll-like receptors; intestinal inflammation
Computational method for predicting Self-Interactions Protein using Recurrent Neural Network from Protein Evolutionary Information
Authors: An Ji-Yong 
Published: February 2020

Abstract
Self-interactions Protein (SIPs) play crucial roles in biological activities of organisms. Many high-throughput methods can be used to identify SIPs. However, these methods are both time-consuming and expensive. How to develop effective computational approaches for identifying SIPs is a challenging task. In the paper, we presented a novelty computational method called RRN-SIFT, which combines the Recurrent Neural Network (RNN) with Scale Invariant Feature Transform (SIFT) to predict SIPs based on protein evolutionary information. The main advantage of the proposed RNN-SIFT model is that it used SIFT for extracting key feature by exploring the evolutionary information embedded in PSI-BLAST-constructed position specific scoring matrix (PSSM) and employed RNN classifier to carry out classification based on extracted features. Extensive experiments show that the RRN-SIFT obtained average accuracy of 94.34% and 97.12% on yeast and human dataset. We also compared our performance with the Back Propagation Neural Network (BPNN), the state-of-the-art support vector machine (SVM) and other exiting methods. By comparing with experimental results, the performance of RNN-SIFT is significantly better than those of the BPNN, SVM and other previous methods in the domain. Therefore, we can come to the conclusion that the proposed RNN-SIFT model is useful tools and can execute incredibly well for predicting SIPs, as well as other bioinformatics tasks. In order to facilitate widely studies and encourage future proteomics research, a freely available web server called RNN-SIFT-SIPs was developed, and is available at http://219.219.62.123:8888/RNNSIFT/ and includes source code and SIPs datasets.
The effect of hydrogenation of epoxy resin on the dispersion of organo-clay in the epoxy resin matrix
Authors: Kang Xiyang , Zhu Qing , Wang Xiaoliang , Zhou Dongshan , Chen zhijun 
Published: February 2020

Abstract
The hydrogenated diglycidylether of Bisphenol A epoxy resin (HDGEBA)was successfully employed to prepare nanocomposites with a more homogeneous distribution of clay, compared to that of bisphenol A epoxy resin (DGEBA)/clay system. Nanocomposites, with amounts up to 7.5 wt% of Organo-clay, were synthetized by means of “slurry-compounding” method and followed by a curing process with cis-1,2-Cyclohexanedicarboxylic anhydride and Glutaric Anhydride as the curing agent. A combination of X-ray diffraction (XRD) and transmission electron microscopy (TEM) were used to characterize the dispersion behavior of organo-clay in epoxy/clay nanocomposites. It was found that, in HDGEBA/clay nanocomposites, organo-clay was uniformly dispersed and even partly exfoliated, whereas, in DGEBA/clay system, large particle aggregates were seen when examined by TEM under lower magnification. Accordingly, the rheology and compatibility experiments were carried out to investigate the interactions within each system. It turned out that, after hydrogenation, HDGEBA was endowed with stronger interactions with organo-clay, thus resulting in the enhanced dispersion behavior, which may generate more chances to be mechanically reinforced by adding inorganic clays.
Rationale and Design of the EMPYREAN Study: Investigation on the Effect of Empagliflozin on Cardiac Sympathetic and Parasympathetic Nerve Activity in Japanese Patients with Type 2 Diabetes
Authors: Motoki Hirohiko , Masuda Izuru , Yasuno Shinji , Oba Koji , Shoin Wataru , Usami Satoru , Saito Yoshihiko , Waki Masako , Komatsu Mitsuhisa , Ueshima Kenji , Nakagawa Yasuaki , Son Cheol , Yonemitsu Shin , Hiramitsu Shinya , Konda Manako , Onishi Katsuya , Kuwahara Koichiro 
Published: February 2020

Abstract
Background: A sodium glucose cotransporter 2 (SGLT2) inhibitor was recently found to reduce heart failure hospitalization in the EMPA-REG OUTCOMES trial. We have hypothesized that autonomic nerve activity may be modulated by SGLT2 inhibition. The current study aims to investigate the impact of empagliflozin on sympathetic and parasympathetic nerve activity in patients with type 2 diabetes mellitus.Methods: This ongoing study is a prospective, randomized, open-label, multicenter investigation of 134 patients with type 2 diabetes mellitus. The patients are randomly allocated to receive either empagliflozin or sitagliptin with the treatment goal of the Japan Diabetes Society guidelines. Ambulatory electrocardiographic monitoring is performed at the baseline and at 12 and 24 weeks of treatment. Analyses of heart rate variability are performed using the MemCalc method, which is a combination of the maximum entropy method for spectral analysis and the non-linear least squares method for square analysis. The primary endpoint is the change in the low frequency (LF; 0.04-0.15 Hz) / high frequency (HF; 0.15-0.4 Hz) ratio from baseline to 24 weeks.Discussion: This investigation on the effect of EMPagliflozin on cardiac sYmpathetic and parasympathetic neRve activity in JapanEse pAtieNts with type 2 diabetes (EMPYREAN study) offers an important opportunity to understand the impact of SGLT2 inhibition on autonomic nerve activity in patients with type 2 diabetes.Trial Registration: UMIN Clinical Trials Registry identifier UMIN000029194. Registered 19 September 2017, https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000033375
Calcium/calmodulin-dependent Protein Kinase IV Limits ER stress and Insulin Resistance through Inhibition of Autophagy in Adipocytes by mTOR/CREB Signaling
Authors: Liu Jiali , Yang Ruihua , Meng Hao , Zhou Ting , He Qian 
Published: February 2020

Abstract
Background: CaMKIV has been identified as a potential regulator of skeletal muscle glucose metabolism and insulin gene expression. However, the mechanism of CaMKIV involved in adipose insulin resistance is not fully understood. Autophagy has to be shown as a potential therapeutic target for endoplasmic reticulum (ER) stress and insulin resistance. The purpose of this study is to investigate the effect of CaMKIV on the ER stress, autophagic function and insulin signaling in tunicamycin induced adipocytes. Methods: In the present study, we first used tunicamycin to establish a cell model of ER stress. Then recombinant CaMKIV and/or targeted-siRNA of CREB and mTOR were added into medium of tunicamycin-induced adipocytes. The ER stress indicators, autophagy activation, mTOR/CREB signaling and insulin sensitivity were analyzed by western blotting or electron microscopy. Results: The results suggested CaMKIV not only reversed tunicamycin-induced ER stress, but also improved impaired insulin sensitivity through regulating abnormal autophagy in adipocytes. Moreover, CaMKIV inhibited activated ER stress and elevated insulin sensitivity in Atg7-/- adipocytes. However, the protective effects of CaMKIV were nullified by suppression of mTOR or CREB in tunicamycin induce adipocytes, suggesting that CaMKIV could inhibit ER stress, suppress autophagy and restore insulin signaling at least partly through mTOR/CREB signaling. Conclusion: We conclude that CaMKIV inhibits ER stress and improves insulin signaling through reduction of autophagy in adipocytes viamTOR/CREB signaling, which could be regarded as novel opportunities for treatment of obesity and type 2 diabetes.