bluuebunny/crossref_metadata_2025_split huggingface dataset:
Studies on fungal communities and functional guilds shift in tea continuous cropping soils by high-throughput sequencing
Authors: Li Yanchun , Li Zhaowei , Arafat Yasir , Lin Wenxiong 
Published: February 2020

Abstract
Purpose
Although soil fungal communities are considered important in tea orchard ecosystems, experimental research on their responses to the long-term continuous ratooning cultivation of tea plants are limited.

Methods
In this study, Illumina high-throughput sequencing technology was employed to investigate soil fungal communities in tea orchards with continuous cropping histories of 0, 1, 10, and 20 years.

Results
Results indicated that the predominant phyla were Ascomycota and Basidiomycota in all tea soil samples. Plant pathogen fungi Alternaria was the most predominant genus in the 10- and 20-year soils and significantly higher than that in the 0- and 1-year soils. FUNGuild revealed that the symbiotrophs in the 1-year soil (8.00%) was markedly higher than those in the 0-, 10-, and 20-year soils (1.43%~2.47%). The saprotrophs in the 20-year soils was approximately two-fold higher than those identified in the 0-, 1-, and 10-year soils. The pathotroph–saprotroph–symbiotroph fungi were higher in the 10- and 20-year soils in comparison to the 0- and 1-year soils as expected. Diversity analysis showed that the indices of Shannon and Simpson in the 1-year soils were higher than those in other treatments. Redundancy analysis suggested that fungal community structure and function were evidently interrelated to pH and exchangeable aluminum in the soils, respectively.

Conclusion
In summary, the long-term continuous ratooning cultivation of tea plants changed the fungal communities in the rhizosphere, enriched saprotrophs and plant pathogens (Alternaria spp.), and reduced beneficial fungi (symbiotrophs). Results of this work can be used to explore reasonable management measures, such as microbial fertilizer application, and eventually relieve the long-term monoculture problems of tea plants.
The cardiac conundrum: a systematic review and bibliometric analysis of authorship in cardiac magnetic resonance imaging studies
Authors: Cuocolo Renato , Ponsiglione Andrea , Dell’Aversana Serena , D’Acierno Ludovica , Lassandro Giulia , Ugga Lorenzo , Romeo Valeria , Vola Elena Augusta , Stanzione Arnaldo , Verde Francesco , Picariello Valentina , Capaldo Iolanda , Pontillo Giuseppe , Cantoni Valeria , Green Roberta , Petretta Mario , Cuocolo Alberto , Imbriaco Massimo 
Published: February 2020

Abstract
Purpose
We aimed to assess the role of radiologists, cardiologists, and other medical and non-medical figures in cardiac magnetic resonance imaging (MRI) research in the last 34 years, focusing on first and last authorship, number of published studies, and journal impact factors (IF).

Methods
Articles in the field of cardiac MRI were considered in this systematic review and retrospective bibliometric analysis. For included studies, the first and last authors were categorized as cardiologists, radiologists/nuclear medicine physicians, medical doctors (MD) with specialties in both cardiology and radiology/nuclear medicine, and other MD and non-MD. Differences in the number of papers published overall and by year and institution location for the first and last author category were assessed. Mean IF differences between author categories were also investigated.

Results
A total of 2053 articles were included in the final analysis. For the first authors (n = 2011), 52% were cardiologists, 22% radiologists/nuclear medicine physicians, 16% other MD, 10% other non-MD, and 1% both cardiologists and radiologists/nuclear medicine physicians. Similarly, the last authors (n = 2029) resulted 54% cardiologists, 22% radiologists/nuclear medicine physicians, 15% other MD, 8% other non-MD, and 2% both cardiologists and radiologists/nuclear medicine physicians. No significant differences due to institution location in the first and last authorship proportions were found. Average journal IF was significantly higher for cardiologist first and last authors when compared to that of radiologists/nuclear medicine physicians (both p < 0.0001).

Conclusion
Over 50% of studies in the field of cardiac MRI published in the last 34 years are conducted by cardiologists.
Lack of a skeletal muscle phenotype in adult human bone marrow stromal cells following xenogeneic-free expansion
Authors: Barisic Dominik , Erb Marita , Follo Marie , Al-Mudaris Dahlia , Rolauffs Bernd , Hart Melanie L. 
Published: February 2020

Abstract
Background
Many studies have elegantly shown that murine and rat bone marrow-derived mesenchymal stromal cells (bmMSCs) contribute to muscle regeneration and improve muscle function. Yet, the ability of transplanted human bmMSCs to manifest myogenic potential shows conflicting results. While human adipose- and umbilical cord-derived MSCs can be differentiated into a skeletal muscle phenotype using horse serum (HS), bmMSCs have only been shown to differentiate towards the skeletal muscle lineage using a complex mixture of cytokines followed by transfection with notch intracellular domain.

Methods
Since xenogeneic-free growth supplements are increasingly being used in the expansion of bmMSCs in clinical trials, we investigated the effects of human plasma and platelet lysate (P/PL) on the expression of neuromuscular markers and whether P/PL-expanded human bmMSCs could be differentiated towards a skeletal myogenic phenotype. Neuromuscular markers were measured using the highly sensitive droplet digital polymerase chain reaction for measuring the expression of Myf5, MyoD, MyoG, ACTA1, Desmin, GAP-43, and Coronin 1b transcripts, by performing immunofluorescence for the expression of Desmin, GAP-43, and MEF2, and flow cytometry for the expression of CD56/neural cell adhesion molecule (NCAM).

Results
Despite that bmMSCs expressed the myogenic regulatory factor (MRF) MEF2 after expansion in P/PL, bmMSCs cultured under such conditions did not express other essential MRFs including Myf5, MyoD, MyoG, or ACTA1 needed for myogenesis. Moreover, HS did not induce myogenesis of bmMSCs and hence did not induce the expression of any of these myogenic markers. P/PL, however, did lead to a significant increase in neurogenic GAP-43, as well as Desmin expression, and resulted in a high baseline expression of the neurogenic gene Coronin 1b which was sustained under further P/PL or HS culture conditions. Fetal bovine serum resulted in equally high levels of GAP-43 and Coronin 1b. Moreover, the proportion of CD56/NCAM-positive bmMSCs cultured in P/PL was 5.9 ± 2.1.

Conclusions
These data suggest that P/PL may prime a small portion of bmMSCs towards an early neural precursor cell type. Collectively, this shows that P/PL partially primes the cells towards a neurogenic phenotype, but does not prime adult human bmMSCs towards the skeletal muscle lineage.
Impact of corticosteroid treatment on clinical outcomes of influenza-associated ARDS: a nationwide multicenter study
Authors:   for Taiwan Severe Influenza Research Consortium (TSIRC) Investigators, Tsai Ming-Ju , Yang Kuang-Yao , Chan Ming-Cheng , Kao Kuo-Chin , Wang Hao-Chien , Perng Wann-Cherng , Wu Chieh-Liang , Liang Shinn-Jye , Fang Wen-Feng , Tsai Jong-Rung , Chang Wei-An , Chien Ying-Chun , Chen Wei-Chih , Hu Han-Chung , Lin Chiung-Yu , Chao Wen-Cheng , Sheu Chau-Chyun 
Published: February 2020

Abstract
Background
Corticosteroid treatment has been widely used in the treatment of septic shock, influenza, and ARDS, although some previous studies discourage its use in severe influenza patients. This multicenter retrospective cohort study conducted in the intensive care units (ICUs) of eight medical centers across Taiwan aims to determine the real-world status of corticosteroid treatment in patients with influenza-associated acute respiratory distress syndrome (ARDS) and its impact on clinical outcomes. Between October 2015 and March 2016, consecutive ICU patients with virology-proven influenza infections who fulfilled ARDS and received invasive mechanical ventilation were enrolled. The impact of early corticosteroid treatment (≥ 200 mg hydrocortisone equivalent dose within 3 days after ICU admission, determined by a sensitivity analysis) on hospital mortality (the primary outcome) was assessed by multivariable logistic regression analysis, and further confirmed in a propensity score-matched cohort.

Results
Among the 241 patients with influenza-associated ARDS, 85 (35.3%) patients receiving early corticosteroid treatment had similar baseline characteristics, but a significantly higher hospital mortality rate than those without early corticosteroid treatment [43.5% (37/85) vs. 19.2% (30/156), p < 0.001]. Early corticosteroid treatment was independently associated with increased hospital mortality in overall patients [adjusted odds ratio (95% CI) = 5.02 (2.39–10.54), p < 0.001] and in all subgroups. Earlier treatment and higher dosing were associated with higher hospital mortality. Early corticosteroid treatment was associated with a significantly increased odds of subsequent bacteremia [adjusted odds ratio (95% CI) = 2.37 (1.01–5.56)]. The analyses using a propensity score-matched cohort showed consistent results.

Conclusions
Early corticosteroid treatment was associated with a significantly increased hospital mortality in adult patients with influenza-associated ARDS. Earlier treatment and higher dosing were associated with higher hospital mortality. Clinicians should be cautious while using corticosteroid treatment in this patient group.
Approximation on a class of Szász–Mirakyan operators via second kind of beta operators
Authors: Nasiruzzaman M. , Rao Nadeem , Srivastava Anshul , Kumar Ravi 
Published: February 2020

AbstractIn the present article, we construct a new sequence of positive linear operators via Dunkl analogue of modified Szász–Durrmeyer operators. We study the moments and basic results. Further, we investigate the pointwise approximation and uniform approximation results in various functional spaces for these sequences of positive linear operators. Finally, we prove the global approximation and A-statistical convergence results for these operators.
Reconstructing the right-hand side of a fractional subdiffusion equation from the final data
Authors: Luc Nguyen Hoang , Baleanu Dumitru , Long Le Dinh , Can Nguyen-Huu 
Published: February 2020

AbstractIn this study, we study an inverse source problem for the time-fractional diffusion equation, where the final data $t = T$t=T are given. We show that our problem is ill-posed in the sense of Hadamard. Applying a truncation method, we give the regularized solution. Finally, convergence estimates under a priori and a posteriori parameter choice rules are proved.
On weak sharp solutions in $(\rho , \mathbf{b}, \mathbf{d})$-variational inequalities
Authors: Treanţă Savin 
Published: February 2020

AbstractIn this paper, weak sharp solutions are investigated for a variational-type inequality governed by $(\rho , \mathbf{b}, \mathbf{d})$(ρ,b,d)-convex path-independent curvilinear integral functional. Moreover, an equivalence between the minimum principle sufficiency property and the weak sharpness property of the solution set associated with the considered variational-type inequality is established.
The appropriateness of antimicrobial use in the outpatient clinics of three hospitals in the Netherlands
Authors: van den Broek Annemieke K. , van Hest Reinier M. , Lettinga Kamilla D. , Jimmink Afra , Lauw Fanny N. , Visser Caroline E. , Prins Jan M. 
Published: February 2020

Abstract
Objectives
Antimicrobial Stewardship Programs commonly have an in-hospital focus. Little is known about the quality of antimicrobial use in hospital outpatient clinics. We investigated the extent and appropriateness of antimicrobial prescriptions in the outpatient clinics of three hospitals.

Methods
From June 2018 to January 2019, we performed ten point prevalence surveys in outpatient clinics of one university hospital and two large teaching hospitals. All prophylactic and therapeutic prescriptions were retrieved from the electronic medical records. Appropriateness was defined as being in accordance with guidelines. Furthermore, we investigated the extent to which the dose was adjusted to renal function and documentation of an antibiotic plan in the case notes.

Results
We retrieved 720 prescriptions for antimicrobial drugs, of which 173 prescriptions (24%) were prophylactic. A guideline was present for 95% of prescriptions, of which the guideline non-adherence rate was 25.6% (n = 42/164) for prophylaxis and 43.1% (n = 224/520) for therapy. Of all inappropriate prescriptions (n = 266), inappropriate prescriptions for skin and soft tissue infections (n = 60/226) and amoxicillin-clavulanic acid (n = 67/266) made up the largest proportion. In only 13 of 138 patients with impaired or unknown renal function the dosage regimen was adjusted. Amoxicillin-clavulanic acid was the drug for which most often renal function was not taken into account. In 94.6% of prescriptions the antibiotic plan was documented.

Conclusions
In hospital outpatient clinics, a substantial part of therapeutics were inappropriately prescribed. Amoxicillin-clavulanic acid was the most inappropriately prescribed drug, due to non-adherence to the guidelines and because dose adjustment to renal function was often not considered.
Whole-genome sequencing to explore nosocomial transmission and virulence in neonatal methicillin-susceptible Staphylococcus aureus bacteremia
Authors: Slingerland Bibi C. G. C. , Vos Margreet C. , Bras Willeke , Kornelisse René F. , De Coninck Dieter , van Belkum Alex , Reiss Irwin K. M. , Goessens Wil H. F. , Klaassen Corné H. W. , Verkaik Nelianne J. 
Published: February 2020

AbstractBackgroundNeonatalStaphylococcus aureus(S. aureus) bacteremia is an important cause of morbidity and mortality. In this study, we examined whether methicillin-susceptibleS. aureus(MSSA) transmission and genetic makeup contribute to the occurrence of neonatalS. aureusbacteremia.MethodsA retrospective, single-centre study was performed. All patients were included who suffered fromS. aureusbacteremia in the neonatal intensive care unit (NICU), Erasmus MC-Sophia, Rotterdam, the Netherlands, between January 2011 and November 2017. Whole-genome sequencing (WGS) was used to characterize theS. aureusisolates, as was also done in comparison to reference genomes. Transmission was considered likely in case of genetically indistinguishableS. aureusisolates.ResultsExcluding coagulase-negative staphylococci (CoNS),S. aureuswas the most common cause of neonatal bacteremia. Twelve percent (n = 112) of all 926 positive blood cultures from neonates grewS. aureus. Based on core genome multilocus sequence typing (cgMLST), 12 clusters of genetically indistinguishable MSSA isolates were found, containing 33 isolates in total (2–4 isolates per cluster). In seven of these clusters, at least two of the identified MSSA isolates were collected within a time period of one month. Six virulence genes were present in 98–100% of all MSSA isolates. In comparison toS. aureusreference genomes, toxin genes encoding staphylococcal enterotoxin A (sea) and toxic shock syndrome toxin 1 (tsst-1) were present more often in the genomes of bacteremia isolates.ConclusionTransmission of MSSA is a contributing factor to the occurrence ofS. aureusbacteremia in neonates.Seaandtsst-1might play a role in neonatalS. aureusbacteremia.
Perceived organizational justice and turnover intention among hospital healthcare workers
Authors: Mengstie Missaye Mulatie 
Published: February 2020

Abstract
Background
Organizational justice is the first virtue in social institutions (J Manage 16:399-432, 1990). It is one of the most determinant factors for an effective utilization of human resources and an essential predictor of organizational success (J Manag Dev 28:457-477, 2009). Employees who perceive fairness are more likely happy with their job and less likely leave their organization (Int J Bus Manage 4:145-154, 2009). Perceived injustice, on the other hand, diminishes motivation of workers to accomplish their duties (Int J Bus Manage 4:145-154, 2009; J Educ Sci Univ Tabriz 2:27-34, 2009). Ethiopia has given emphasis to the expansion of health institutions and increasing the number of health professionals. Despite this, little emphasis has been given the human resource aspect of the health sector. Therefore, this study aims to investigate organizational justice perceptions and turnover intentions among healthcare workers in Amhara region.

Methods
One hundred ninety seven healthcare workers participated in the study. Data were collected through self- report questionnaire and semi-structured interview. The quantitative data were analyzed through MANOVA, multiple regression, and independent samples t-test. The qualitative data were analyzed through thematic analysis.

Results
The results of this study revealed that healthcare workers in the public hospitals held low perceived distributive, procedural, interpersonal and informational justice. Similarly, private hospitals healthcare workers had low perceptions on distributive and procedural justice. On the contrary, healthcare workers in private hospitals reported high perception of fairness on interpersonal and informational justice aspects. Both public and private hospital healthcare workers had high turnover intention. The result revealed significant difference in organizational justice perceptions between private and public hospital healthcare workers (F (4, 182) = 9.17; p < .05; partial η2 =. 168). Organizational justice dimensions (distributive, procedural, interpersonal and informational justice) significantly contributed an additional 9.9% variation in turnover intention (R 2 change = .099, F (4,170) = 4.86, p < .05). Distributive justice was the most important predictor of turnover intention (β = −.23, p < .05).

Conclusion
Organizational justice perceptions of healthcare workers significantly predicted turnover intention. Hence, organizational justice should be given due emphasis in designing and implementing policies and strategies of human resource management.
Planning tourist infrastructures to regenerate marginalised territories: the study case of North Sardinia, Italy
Authors: Sechi Ludovica , Moscarelli Rossella , Pileri Paolo 
Published: February 2020

AbstractEuropean marginalised areas are affected by an alarming rate of abandonment and depopulation. Thus, they are therefore object of specific European policies aiming to their reactivation. Official guidelines, both European and Italian, indicate sustainable tourism as one of the main triggers able to activate positive processes. This research experiments a suggestion in a case study, the North of Sardinia. The coastal areas of Sardinia are popular tourist destinations, particularly in the North with the famous Costa Smeralda. Therefore, it is worthy to imagine a way to allow them to discover the inner territories of the region too.
A single ultrasensitive assay for detection and discrimination of tau aggregates of Alzheimer and Pick diseases
Authors: Metrick Michael A. , Ferreira Natália do Carmo , Saijo Eri , Kraus Allison , Newell Kathy , Zanusso Gianluigi , Vendruscolo Michele , Ghetti Bernardino , Caughey Byron 
Published: February 2020

AbstractMultiple neurodegenerative diseases are characterized by aggregation of tau molecules. Adult humans express six isoforms of tau that contain either 3 or 4 microtubule binding repeats (3R or 4R tau). Different diseases involve preferential aggregation of 3R (e.g Pick disease), 4R (e.g. progressive supranuclear palsy), or both 3R and 4R tau molecules [e.g. Alzheimer disease and chronic traumatic encephalopathy]. Three ultrasensitive cell-free seed amplification assays [called tau real-time quaking induced conversion (tau RT-QuIC) assays] have been developed that preferentially detect 3R, 4R, or 3R/4R tau aggregates in biospecimens. In these reactions, low-fg amounts of a given self-propagating protein aggregate (the seed) are incubated with a vast excess of recombinant tau monomers (the substrate) in multi-well plates. Over time, the seeds incorporate the substrate to grow into amyloids that can then be detected using thioflavin T fluorescence. Here we describe a tau RT-QuIC assay (K12 RT-QuIC) that, using a C-terminally extended recombinant 3R tau substrate (K12CFh), enables sensitive detection of Pick disease, Alzheimer disease, and chronic traumatic encephalopathy seeds in brain homogenates. The discrimination of Pick disease from Alzheimer disease and chronic traumatic encephalopathy cases is then achieved through the quantitative differences in K12 RT-QuIC assay thioflavin T responses, which correlate with structural properties of the reaction products. In particular, Fourier transform infrared spectroscopy analysis of the respective K12CFh amyloids showed distinct β-sheet conformations, suggesting at least partial propagation of the original seed conformations in vitro. Thus, K12 RT-QuIC provides a single assay for ultrasensitive detection and discrimination of tau aggregates comprised mainly of 3R, or both 3R and 4R, tau isoforms.
A preliminary study of Liu Kang’s palette and the discovery and interpretation of hidden paint layers
Authors: Lizun Damian 
Published: February 2020

Abstract
This paper presents an overview of the results from the ongoing study on the materials and painting technique of the pioneering Singapore artist—Liu Kang. The paintings Zuo La Lu and Nude, which represent two of Liu Kang’s early and distinct artistic phases—Paris and Shanghai—were investigated for the first time using a combination of non- and micro-invasive techniques. The aim of this study was to identify the main pigments used by the artist and to add to the existing research on the artist’s painting methods. The results show that the majority of pigments used in both paintings are similar and include Prussian blue, ultramarine, viridian, strontium yellow, chrome yellow, cadmium yellow, iron oxides, lead, zinc and barium whites, bone black and organic red. Particularly interesting is the predominance of lead white in Zuo La Lu and zinc white in Nude. A comparison of the ground layers also indicates the presence of a lead white admixture in Zuo La Lu while its absence in Nude may point to a characteristic difference between two artistic phases, possibly determined by the availability of materials. The imaging methods revealed hidden compositions: the view of a canal house behind Zuo La Lu, and a mystery outdoor view behind Nude. Although these investigative methods permitted some visualisation of the discarded compositions, it remains difficult to determine their details.
Understanding the development trends of big data technologies: an analysis of patents and the cited scholarly works
Authors: Saheb Tahereh , Saheb Tayebeh 
Published: February 2020

AbstractBig data innovation is a key instrument for economic and social development and for the creation of new business opportunities. This study analyzes the patenting activities of global jurisdictions in the field of big data; as well as the scholarly cited works. We collected 13,112 patent applications between 1992 and 2019, and 642 cited scholarly works by the patents. Our findings report on the development trends in big data technologies, as well as on the link between patenting activities and the cited scientific works. It also analyzes and visualizes the social networks embedded in patents and the cited scientific works; thus, it reveals the patenting activities of global jurisdictions on big data technologies, the strength of the interaction of various agents, such as inventors and applicants within social networks, and the link between patents and the scientific world. This study shows that most of the big data patent applications filed fall within the IPC category of information retrieval, database structures, and file system structures. The majority of the applicants and inventors of the patents filed are Chinese companies and individuals. Scientific fields with stronger connections within the network of co-fields are computer science and medicine. There is a weak link between inventions and scientific works.