bluuebunny/crossref_metadata_2025_split huggingface dataset:
Municipal waste incineration fly ashes: from a multi-element approach to market potential evaluation
Authors: Fabricius Anne-Lena , Renner Monika , Voss Marieke , Funk Michael , Perfoll Anton , Gehring Florian , Graf Roberta , Fromm Stephan , Duester Lars 
Published: June 2020

Abstract
Background
Fly ashes from municipal solid waste incineration contain significant amounts of (technology critical) elements. Processes to recover Cu or Zn are already in practice, but it still remains difficult to evaluate the full secondary resource potential of the ashes. One reason is the absence of a worldwide comparable analytical basis for detailed market analyses. To encounter this, (i) an advice on how to analyse 65 elements after microwave-assisted digestion by ICP-OES and ICP-MS is delivered, (ii) the heterogeneity (hours to annual cycle) is evaluated for a incineration plant, (iii) leaching efficiency with three different eluents and (iv) the market potential of the elements as commodities are evaluated.

Results and conclusions
Aqua regia digestion was found to be sufficient to evaluated the recovery potential; except for the mass constituents Al, Si, Sn, Ti and the trace components Cr, Hf, Nb, U and W, for which HF-containing digestions delivered better recoveries. On different time scales, ashes were very homogenous and HCl- as well as H2SO4-supported leaching delivered, satisfying results within an hour (exceptions are, e.g., Bi and Sb). By applying characterisation factors of the life cycle assessment impact category “Resource depletion—minerals and metals” supplemented by the list of critical raw materials of the EU: Ag, Bi, Cd, Ga, In and Sb are most interesting elements to be recovered in future activities.
Identification of novel cerebrospinal fluid biomarker candidates for dementia with Lewy bodies: a proteomic approach
Authors: van Steenoven Inger , Koel-Simmelink Marleen J. A. , Vergouw Leonie J. M. , Tijms Betty M. , Piersma Sander R. , Pham Thang V. , Bridel Claire , Ferri Gian-Luca , Cocco Cristina , Noli Barbara , Worley Paul F. , Xiao Mei-Fang , Xu Desheng , Oeckl Patrick , Otto Markus , van der Flier Wiesje M. , de Jong Frank Jan , Jimenez Connie R. , Lemstra Afina W. , Teunissen Charlotte E. 
Published: June 2020

Abstract
Background
Diagnosis of dementia with Lewy bodies (DLB) is challenging, largely due to a lack of diagnostic tools. Cerebrospinal fluid (CSF) biomarkers have been proven useful in Alzheimer’s disease (AD) diagnosis. Here, we aimed to identify novel CSF biomarkers for DLB using a high-throughput proteomic approach.

Methods
We applied liquid chromatography/tandem mass spectrometry with label-free quantification to identify biomarker candidates to individual CSF samples from a well-characterized cohort comprising patients with DLB (n = 20) and controls (n = 20). Validation was performed using (1) the identical proteomic workflow in an independent cohort (n = 30), (2) proteomic data from patients with related neurodegenerative diseases (n = 149) and (3) orthogonal techniques in an extended cohort consisting of DLB patients and controls (n = 76). Additionally, we utilized random forest analysis to identify the subset of candidate markers that best distinguished DLB from all other groups.

Results
In total, we identified 1995 proteins. In the discovery cohort, 69 proteins were differentially expressed in DLB compared to controls (p < 0.05). Independent cohort replication confirmed VGF, SCG2, NPTX2, NPTXR, PDYN and PCSK1N as candidate biomarkers for DLB. The downregulation of the candidate biomarkers was somewhat more pronounced in DLB in comparison with related neurodegenerative diseases. Using random forest analysis, we identified a panel of VGF, SCG2 and PDYN to best differentiate between DLB and other clinical groups (accuracy: 0.82 (95%CI: 0.75–0.89)). Moreover, we confirmed the decrease of VGF and NPTX2 in DLB by ELISA and SRM methods. Low CSF levels of all biomarker candidates, except PCSK1N, were associated with more pronounced cognitive decline (0.37 < r < 0.56, all p < 0.01).

Conclusion
We identified and validated six novel CSF biomarkers for DLB. These biomarkers, particularly when used as a panel, show promise to improve diagnostic accuracy and strengthen the importance of synaptic dysfunction in the pathophysiology of DLB.
Effects of cashew gum and nanoparticles on cooled stallion semen
Authors: Loureiro Kahynna Cavalcante , Lima-Verde Isabel Bezerra , Johannisson Anders , Ntallaris Theodoros , Jager Alessandro , Štěpánek Petr , da Costa Mendonça Marcelo , Severino Patrícia , Morrell Jane M. 
Published: June 2020

Abstract
Background
Cryopreservation of stallion spermatozoa tends to cause plasma membrane damage due to the low ratio of cholesterol to phospholipids. Gums have been suggested as an alternative cryoprotectant to glycerol for stallion spermatozoa. Therefore, the present experiment was designed to verify whether the effect of addition of cashew gum (CG), or nanoparticles (NP) containing CG, to the extender before cooling on sperm quality in stallion semen. Ejaculates from 6 stallions were extended and split between six treatment groups (control, a-tocopherol [TOC], CG1, CG0.5, NP1 and NP0.5), stored in cryotubes at 4 °C.

Results
Aliquots were analysed by computer-assisted sperm motility analysis on the day of collection, and after 24 h and 48 h of cold storage. After 48 h, the total motility with NP1 (78.53 + 6.31%) was similar to control 85.79 + 6.31% at 0 h. The same pattern was observed for progressive motility. Membrane integrity assessed by flow cytometer was similar between control, TOC and G1 at all storage times. The DNA fragmentation in the control group increased at all time points, whereas chromatin integrity was maintained after 24 h in TOC and NP0.5 compared to 0 h. There was no increase in the proportion of live spermatozoa producing hydrogen peroxide, but there was a tendency for an increased proportion of spermatozoa in the live superoxide category in CG1 after 24 h cooled storage.

Conclusions
The addition of CG or CG-derived NP to extender for stallion semen was not harmful to the sperm cells.
Urbanisation, risk stratification and house infestation with a major vector of Chagas disease in an endemic municipality of the Argentine Chaco
Authors: Gaspe María Sol , Fernández María del Pilar , Cardinal Marta Victoria , Enriquez Gustavo Fabián , Rodríguez-Planes Lucía Inés , Macchiaverna Natalia Paula , Gürtler Ricardo Esteban 
Published: June 2020

Abstract
Background
The occurrence of the major vectors of Chagas disease has historically been linked to poor rural housing, but urban or peri-urban infestations are increasingly being reported. We evaluated a simple risk index to detect houses infested with Triatoma infestans and tested whether house infestation and vector abundance increased across the urban-to-rural gradient in Avia Terai, an endemic municipality of the Argentine Chaco; whether the association between infestation and selected ecological determinants varied across the gradient; and whether urban and peri-urban infestations were associated with population settlement history.

Methods
We conducted a screening survey of house infestation in 2296 urban, peri-urban and rural dwellings to identify high-risk houses based on a simple index, and then searched for triatomines in all high-risk houses and in a systematic sample of low-risk houses.

Results
The risk index had maximum sensitivity and negative predictive value, and low specificity. The combined number of infested houses in peri-urban and urban areas equalled that in rural areas. House infestation prevalence was 4.5%, 22.7% and 42.4% across the gradient, and paralleled the increasing trend in the frequency of domestic animals and peridomestic structures. Multiple logistic regression analysis showed that house infestation was positively and significantly associated with the availability of poultry and bug refuges in walls, and was negatively associated with domestic insecticide use. Several pieces of evidence, including absence of spatial aggregation of house infestation, support that T. infestans has been a long-established occupant of urban, peri-urban and rural settings in Avia Terai.

Conclusions
An integrated vector management strategy targeting chicken coops and good husbandry practices may provide more cost-effective returns to insecticide-based vector elimination efforts.
Excretory/secretory products of Angiostrongylus cantonensis fifth-stage larvae induce endoplasmic reticulum stress via the Sonic hedgehog pathway in mouse astrocytes
Authors: Chen Kuang-Yao , Chen Yi-Ju , Cheng Chien-Ju , Jhan Kai-Yuan , Wang Lian-Chen 
Published: June 2020

Abstract
Background
Angiostrongylus cantonensis is an important food-borne zoonotic parasite. Humans are non-permissive hosts, and this parasite develops into fifth-stage larvae (L5) in the brain and subarachnoid cavity and then induces eosinophilic meningitis and eosinophilic meningoencephalitis. Excretory/secretory products (ESPs) are valuable targets for the investigation of host-parasite interactions. These products contain a wide range of molecules for penetrating defensive barriers and avoiding the immune response of the host. Endoplasmic reticulum (ER) stress has been found to be associated with a wide range of parasitic infections and inflammation. ER stress can increase cell survival via the activation of downstream signalling. However, the mechanisms of ER stress in A. cantonensis infection have not yet been clarified. This study was designed to investigate the molecular mechanisms of ER stress in astrocytes after treatment with the ESPs of A. cantonensis L5.

Results
The results demonstrated that A. cantonensis infection activated astrocytes in the mouse hippocampus and induced the expression of ER stress-related molecules. Next, the data showed that the expression of ER stress-related molecules and the Ca2+ concentration were significantly increased in activated astrocytes after treatment with the ESPs of L5 of A. cantonensis. Ultimately, we found that ESPs induced GRP78 expression via the Sonic hedgehog (Shh) signalling pathway.

Conclusions
These findings suggest that in astrocytes, the ESPs of A. cantonensis L5 induce ER stress and that the Shh signalling pathway plays an important role in this process.
Limited MOMP, ATM, and their roles in carcinogenesis and cancer treatment
Authors: Bao Xuhui , Liu Xinjian , Li Fang , Li Chuan-Yuan 
Published: June 2020

AbstractLimited mitochondria outer membrane permeability (MOMP) is a novel biological process where mammalian cells initiate the intrinsic apoptosis pathway with increased mitochondrial permeability but survive. One of the major consequences of limited MOMP is apoptotic endonuclease-induced DNA double strand breaks. Recent studies indicate that these DNA double stand breaks and ensuing activation of DNA damage response factors such as ATM play important but previously underappreciated roles in carcinogenesis and tumor growth. Furthermore, novel non-canonical roles of DNA repair factors such as ATM in tumor growth and treatment are also emerging. In this review, we try to summarize recent findings on this newly revealed link between DNA double strand break repair and cell death pathways.
A joint optimization scheme of content caching and resource allocation for internet of vehicles in mobile edge computing
Authors: Zhang Mu , Wang Song , Gao Qing 
Published: June 2020

AbstractIn a high-speed free-flow scenario, a joint optimization scheme for content caching and resource allocation is proposed based on mobile edge computing in Internet of Vehicles. Vehicle trajectory prediction provides the basis for the realization of vehicle-cloud collaborative cache. By pre-caching the business data of requesting vehicles to edge cloud networks and oncoming vehicles, requesting vehicles can obtain data through V2V link and V2I link at the same time, which reduces the data acquisition delay. Therefore, this paper considers the situation where bandwidth of V2I and V2V link and the total amount of edge cloud caches are limited. Then, the bandwidth and cache joint allocation strategy to minimize the weighted average delay of data acquisition is studied. An edge cooperative cache algorithm based on deep deterministic policy gradient is further developed. Different from Q-learning and deep reinforcement learning algorithms, the proposed cache algorithm can be well applied to variable continuous bandwidth allocation action space. Besides, it effectively improves the convergence speed by using interactive iteration of value function and strategy function. Finally, the simulation results of vehicle driving path at the start and stop are obtained by analyzing real traffic data. Simulation results show that the proposed scheme can achieve better performance than several other newer cooperative cache schemes.
Laparoscopic partial splenectomy for splenic lymphangioma: a case report
Authors: Kimura Kotaro , Kurashima Yo , Tanaka Kimitaka , Nakanishi Yoshitsugu , Asano Toshimichi , Ebihara Yuma , Noji Takehiro , Murakami Soichi , Nakamura Toru , Tsuchikawa Takahiro , Okamura Keisuke , Shichinohe Toshiaki , Kanno-Okada Hiromi , Hirano Satoshi 
Published: June 2020

Abstract
Background
Lymphangioma is a benign malformation of the lymphatic system and is often found in the neck and axilla, the orbit, the mediastinum, etc. However, isolated splenic lymphangioma is a rare disease in young women, and its treatment is controversial. We report a case of laparoscopic partial splenectomy for isolated splenic lymphangioma in a young woman.

Case presentation
An 18-year-old woman with mild epigastralgia was admitted to a nearby hospital. Abdominal ultrasound detected a 6-cm mass confined to the upper pole of the spleen; thereafter, she was referred to our department for surgical treatment. Although a benign tumor, we decided to resect it because of her symptoms. To preserve part of the normal spleen, laparoscopic partial splenectomy was performed with a co-axial approach using four ports and a liver retractor in the lithotomy position. After dissection around the spleen hilum, we identified that the tumor was being fed from the splenic vessels of the upper pole and severed the branch. Postoperatively, the patient showed no complications and was discharged on postoperative day 8 without symptoms. Pathological examination revealed splenic lymphangioma, which is rare in young women. No recurrence was seen 1 year after surgery, and a computed tomography scan showed no problems with the remaining spleen.

Conclusions
In our experience of laparoscopic partial splenectomy for a young woman with an isolated splenic lymphangioma, we determined that laparoscopic partial splenectomy is a safe, effective, and valuable option for the treatment of benign splenic tumors.
Liver metastasis from papillary thyroid carcinoma treated by laparoscopic hepatectomy 43 years after resection of the primary tumor: a case report
Authors: Tsukagoshi Mariko , Harimoto Norifumi , Araki Kenichiro , Kubo Norio , Watanabe Akira , Igarashi Takamichi , Ishii Norihiro , Yajima Toshiki , Sano Takaaki , Shirabe Ken 
Published: June 2020

Abstract
Background
Papillary thyroid carcinoma (PTC) generally has a good prognosis, while liver metastasis from PTC is rare and difficult to diagnose.

Case presentation
A 67-year-old woman was diagnosed with PTC and underwent a left hemithyroidectomy 43 years ago. Two years ago, thoracoscopic right middle lobectomy was performed for a lung tumor, and pathology reports confirmed a metastatic lung tumor of PTC. The patient was followed up regularly with computed tomography, and a liver tumor measuring 16 mm was found in the lateral segment of the liver. Fluorodeoxyglucose positron emission tomography (FDG-PET) was negative for liver tumor. However, FDG uptake was observed at the nodule outside the right lobe of the thyroid gland, suggesting metastasis to the right supraclavicular lymph node. Laparoscopic partial S2 hepatectomy was performed without complications. The final diagnosis was metastatic liver tumor from PTC, and the surgical margins were free of tumor. Postoperatively, the patient underwent complementary thyroidectomy and right supraclavicular lymph node dissection, followed by I-131 ablation. The final diagnosis was PTC of 7 mm and 4 mm and lymph node metastasis of 9 mm. The patient is currently doing well and has had no recurrence 1 year after surgery.

Conclusions
This case demonstrates that liver metastases from PTC may be found after long-term follow-up, and liver resection might be the most appropriate treatment.
Investigating gender-specific effects of familial risk for attention-deficit hyperactivity disorder and other neurodevelopmental disorders in the Swedish population
Authors: Martin Joanna , Ghirardi Laura , Chen Qi , Hartman Catharina A. , Rosenqvist Mina A. , Taylor Mark J. , Birgegård Andreas , Almqvist Catarina , Lichtenstein Paul , Larsson Henrik 
Published: June 2020

Background
Many psychiatric disorders show gender differences in prevalence. Recent studies suggest that female patients diagnosed with anxiety and depression carry more genetic risks related to attention-deficit hyperactivity disorder (ADHD) compared with affected males.

Aims
In this register-based study, we aimed to test whether female patients who received clinical diagnoses of anxiety, depressive, bipolar and eating disorders are at higher familial risk for ADHD and other neurodevelopmental disorders, compared with diagnosed male patients.

Method
We analysed data from a record-linkage of several Swedish national registers, including 151 025 sibling pairs from 103 941 unique index individuals diagnosed with anxiety, depressive, bipolar or eating disorders, as well as data from 646 948 cousin pairs. We compared the likelihood of having a relative diagnosed with ADHD/neurodevelopmental disorders in index males and females.

Results
Female patients with anxiety disorders were more likely than affected males to have a brother with ADHD (odd ratio (OR) = 1.13, 95% CI 1.05–1.22). Results for broader neurodevelopmental disorders were similar and were driven by ADHD diagnoses. Follow-up analyses revealed similar point estimates for several categories of anxiety disorders, with the strongest effect observed for agoraphobia (OR = 1.64, 95% CI 1.12–2.39). No significant associations were found in individuals with depressive, bipolar or eating disorders, or in cousins.

Conclusions
These results provide modest support for the possibility that familial/genetic risks for ADHD may show gender-specific phenotypic expression. Alternatively, there could be gender-specific biases in diagnoses of anxiety and ADHD. These factors could play a small role in the observed gender differences in prevalence of ADHD and anxiety.
Does ethnic density influence community participation in mass participation physical activity events? The case of parkrun in England
Authors: Smith Robert , Schneider Paul , Bullas Alice , Haake Steve , Quirk Helen , Cosulich Rami , Goyder Elizabeth 
Published: June 2020

Background: parkrun has been successful in encouraging people in England to participate in their weekly 5km running and walking events. However, there is substantial heterogeneity in parkrun participation across different communities in England: after controlling for travel distances, deprived communities have significantly lower participation rates. Methods: This paper expands on previous findings by investigating disparities in parkrun participation by ethnic density. We combined geo-spatial data available through the Office for National Statistics with participation data provided by parkrun, and fitted multivariable Poisson regression models to study the effect of ethnic density on participation rates at the Lower layer Super Output Level. Results: We find that areas with higher ethnic density have lower participation rates. This effect is independent of deprivation. Conclusions: An opportunity exists for parkrun to engage with these communities and reduce potential barriers to participation.
Modelamiento de nichos ecológicos de flora amenazada para escenarios de cambio climático en el departamento de Tacna - Perú
Authors: Navarro Guzmàn Marco Alberto , Jove Chipana Cesar Augusto , Ignacio Apaza Javier Máximo 
Published: June 2020

A pesar de la numerosa información científica sobre cambio climático mundial, no existen estudios que demuestren los efectos sobre la biodiversidad a menor escala. Por ello, utilizando 19 variables bioclimáticas, cinco de radiación solar, altitud, software especializado (MaxEnt) y coordenadas geográficas de presencia de cinco especies de flora categorizada verificadas en campo se modelaron sus nichos ecológicos actuales y proyectados a los cuatro escenarios futuros de emisiones (2050 y 2070). Se demostró que el de Buddleja coriacea disminuirá en más del 80 % por las variaciones futuras de precipitación y temperatura consecuencia del cambio climático, mientras que Carica candicans, Haplorhus peruviana, Kageneckia lanceolata y Weberbauerella brongniartioides se mantendrán e incrementarán, deduciéndose que sus categorías de amenaza derivan principalmente por actividad antropogénica. Estos modelos mejoran significativamente la comprensión del funcionamiento ecosistémico, otorgando información útil para diseñar políticas y acciones de conservación que orienten la gestión territorial hacia la estrategia de adaptación basada en ecosistemas.
The role of infectious pathogens in etiopathogenesis of degenerative intervertebral disc disease
Authors: Alexanyan M. M. , Aganesov A. G. , Pogosyan E. L. , Mrugova T. M. , Chukina M. A. , Ivanova A. G. , Gemdzhian E. G. 
Published: June 2020

Objective. To analyze the presence of infectious pathogens in the intervertebral disc tissues as possible pathogenetic factor in the development of degenerative diseases of the spine. Material and Method. In 2017–2018, a prospective exploratory research was conducted, which included 64 patients with degenerative disease of the spine who met the following inclusion criteria: the presence of clinically and instrumentally proven hernias of the intervertebral discs in the lumbosacral spine, the absence of a history of surgical interventions on the spine, non-intake of antibacterial drugs for the year before surgery, and Modic I and /or II type changes on MRI. All patients underwent interventions with sampling of intervertebral disc material; a total of 80 discs were examined. The obtained materials were subjected to microbiological and histopathological studies.Results. Out of 64 patients, only 1 (1.6 %) revealed the presence of bacterial flora in the intervertebral disc tissue (Propionibacterium acnes strain). It can be assumed that the identified bacterial flora is the result of contamination. It is also possible that the infectious agent joined already during the development of degenerative disease of the spine. If the bacterial flora was a pathogenetic factor, then it can be assumed that the pathogenesis of the disease is polyetiological and includes the infectious process as a rare event.Conclusions. Given the high interest of specialists in the pathogenesis of degenerative diseases, it seems appropriate to conduct further studies in this direction using various laboratory diagnostic methods with high sensitivity and specificity.