bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short term outcomes of preterm infants following antenatal corticosteroid treatment for childbearing women at 34 (0/7) to 36 (6/7) weeks: do the advantages outweigh the disadvantages?
Authors: Hu Zhimei , Deng Xiaochun , Liu Honglin , Xie Lili , Liang Bingwei , Yang Jianhui , Lin Xuemei , Chen Peishan , Huang Yuejun 
Published: August 2020

Abstract
Background The effects of maternal antenatal corticosteroid (ACS) treatment, for fetal maturation, on the short-term outcome of late preterm infants are unclear. Methods This is a retrospective cohort study conducted in the Second Affiliated Hospital of Shantou University Medical College. Data of pregnant women who gave birth between 34 (0/7) to 36 (6/7) weeks gestation from January 2014 to June 2019 were collected. Nine short-term outcomes of preterm infants from mothers who received ACS treatment were compared to preterm infants from mothers who did not receive ACS treatment. Results In total, 1393 pregnant women (of whom 757 accepted ACS treatment before delivery) and 1472 preterm infants were eligible for analysis. The results were as follows: (1) ACS administration to pregnant women at high risk for giving birth between 34 (0/7) to 36 (6/7) weeks pregnancy , was related to decreasing cost and infant inpatient time (slope was -0.784, P =0.026 and slope was -933.173, P =0.001, respectively); (2) lack of maternal ACS treatment was an independent risk factor for neonatal respiratory distress syndrome (RR=0.548, 95%CI=0.332～0.906); (3) use of maternal ACS did not increase risk of neonatal pneumonia, neonatal hypoglycemia, neonatal sepsis, necrotizing enterocolitis of newborns, neonatal intracranial hemorrhage, and hypoxic-ischemic encephalopathy in preterm infants. Conclusions Use of ACS for pregnant women at risk for giving birth between 34 (0/7) to 36 (6/7) weeks pregnancy may have more advantages than disadvantages. Our study provides evidence-based medicine for clinicians to make ACS treatment choices for pregnant women with risk of giving birth between 34 (0/7) to 36(6/7) weeks gestation.
Pelvic Rotation Parameters Related to In-Brace Correction in Patients with Idiopathic Scoliosis
Authors: li kepeng , Miao Jun , Jing-an Zhang 
Published: August 2020

Abstract
BACKGROUND: To identify the pelvic parameters affecting in-brace correction (IBC) in patients with idiopathic scoliosis (IS). METHODS: Patients with IS receiving Chêneau brace treatment in our scoliosis center from January 2019 to November 2019 were retrospectively analyzed. Pelvic rotation parameters, including Pelvic incidence (PI), Sacral slope (SS), pelvic tilt (PT), L/R ratio, were collected. Other radiographic data, such as Risser sign, coronal and sagittal balance, curve location, kyphosis, lordosis of each patient was also recorded to analyze their correlations with IBC. Correlation analyses were performed to identify the classified variables influencing IBC. The principal component analysis was used to extract common factors of radiographic parameters to eliminate interaction effects. The linear regression equation was established using principal components, the variables influencing IBC were identified.RESULTS: A cohort of 44 patients with IS (36 girls and 8 boys) were included in the present study. The mean IBC was 49.87% (range, 3%∽100%). IBC of lumbar IS was negatively correlated with apical rotate factor (ARF, B=-0.385), mainly consisted of pelvic coronal plane rotation (PCPR, 0.449), cobb angle (CA,0.575), apical vertebral rotation (AVR, 0.918), and pelvic rotate factor (PRF, B=-0.387), mainly consisted of PT (0.861), PI (0.728), PCPR (-0.570). The regression equation of lumbar IS had statistical significance (F=6.500, P=0.005，R2=0.317). Whereas statistically significance was not found in the regression equation of thoracic IS (F=2.913, P=0.106). The remaining parameters were not related to IBC.CONCLUSIONS: For lumbar IS, ARF and PRF have negative effects on IBC, Coronal and sagittal rotation of the pelvis is related to IBC.
High sensitivity of one-step real-time reverse transcription quantitative PCR to detect low virus titers in large mosquito pools
Authors: Tang Zhaoyang , Yamada Hanano , Kraupa Carina , Canic Sumejja , Busquets Núria , Talavera Sandra , Jiolle Davy , Vreysen Marc J. B. , Bouyer Jérémy , Abd-Alla Adly 
Published: August 2020

Abstract
BackgroundMosquitoes are the deadliest animals in the world. Their ability to carry and spread diseases to humans causes millions of deaths every year. Due to the lack of efficient vaccines, the control of mosquito-borne diseases primarily relies on the management of the vector. Traditional control methods such as source reduction and chemical insecticides, have proven to be sufficient to prevent the proliferation and spread of mosquito populations. The sterile insect technique (SIT) is an additional control method that can be combined with other control tactics to suppress specific mosquito populations. The SIT requires the mass-rearing and release of sterile males with the aim to induce sterility in the wild female population. Samples collected from the environment for laboratory colonization, as well as the released males, should be free from mosquito-borne viruses (MBV). Therefore, efficient detection methods with defined detection limits for MBV are required. Although a one-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) method was developed to detect arboviruses in human and mosquito samples, its detection limit in mosquito samples has yet to be defined.MethodsWe evaluated the detection sensitivity of one step RT-qPCR for targeted arboviruses in large mosquito pools, using pools of non-infected mosquitoes of various sizes (165, 320 and 1600 mosquitoes) containing one infected mosquito body with defined virus titers of chikungunya virus (CHIKV), usutu virus (USUV), West Nile virus (WNV) and Zika virus (ZIKV).ResultsCHIK, USUV, ZIKV, and WNV virus were detected in all tested pools using the RT-qPCR assay. Moreover, in the largest mosquito pools (1600 mosquitoes), RT-qPCR was able to detect the targeted viruses using different total RNA quantities (10, 1, 0.1 ng per reaction) as a template. Correlating the virus titer with the total RNA quantity allowed the prediction of the maximum number of mosquitoes per pool in which the RT-qPCR can theoretically detect the virus infection.ConclusionMosquito borne viruses can be reliably detected by RT-qPCR assay in pools of mosquitoes exceeding 1000 specimens. This will represent an important step to expand pathogen-free colonies for mass-rearing sterile males for programmes that have an SIT component by reducing the time and the manpower needed to conduct this quality control process.
Probiotics ameliorate pioglitazone-associated bone loss in diabetic rats
Authors: Gholami Ahmad , Dabbaghmanesh Mohammad Hossein , Ghasemi Younes , Talezadeh Pedram , Koohpeyma Farhad , Montazeri-Najafabady Nima 
Published: August 2020

Abstract
Background: Pioglitazone, as a PPAR gamma agonist, is used for the management of type 2 diabetes mellitus. Nevertheless, evidence showed that the therapeutic modulation of PPAR gamma activity using pioglitazone might be linked with bone mass reduction and fracture risk in type 2 diabetes mellitus patients. The objective of the current research was to inspect the preventive role of some types of probiotic strains, including (Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus casei, Bifidobacterium longum, and Bacillus coagulans) against pioglitazone-induced bone loss.Methods: Streptozotocin (60 mg/kg) was administered for diabetes induction. Diabetic rats were fed orally with pioglitazone (300 mg/kg) and probiotics (1×109 CFU/ml/day) alone and in combination for four weeks. Dual-energy X-ray absorptiometry (DXA) was used to assess BMD, BMC, and area of the femur, spine, and tibia at the end of the experiment. Serum glucose, serum calcium (Ca), alkaline phosphatase (ALP), phosphorus (P), Blood urea nitrogen (BUN), creatinine, and urine calcium were also analyzed. Results: Administration of pioglitazone and probiotics alone and, in combination, significantly reduced elevated blood glucose. Pioglitazone treatment significantly increased urinary calcium and BUN and decreased ALP and creatinine. Co-treatment of probiotics with pioglitazone significantly decreased urinary calcium, creatinine, and ALP. Pioglitazone showed detrimental effects on femur-BMD, whereas treatment with probiotics remarkably ameliorated these effects. Among the tested probiotics, Bifidobacterium longum displayed the best protective effects on pioglitazone-induced bone loss in diabetic rats. Conclusion: This study suggests probiotic supplementation in diabetic patients on pioglitazone regime could be considering as an excellent strategy to ameliorate bone loss induced by pioglitazone.
Atomic Ring Invariant and Modified CANON Extended Connectivity Algorithm for Symmetry Perception in Molecular Graphs and Rigorous Canonicalization of SMILES
Authors: Krotko Dmytro G. 
Published: August 2020

Abstract
We propose new invariant (the product of the corresponding primes for the ring size of each bond of an atom) as a simple unambiguous ring invariant of an atom that allows distinguishing symmetry classes in the highly symmetrical molecular graphs using traditional local and distance atom invariants. Also, we propose modifications of Weininger’s CANON algorithm to avoid its ambiguities (swapping and leveling ranks, incorrect determination of symmetry classes in non-aromatic annulenes, arbitrary selection of atom for breaking ties). The atomic ring invariant and the Modified CANON algorithm allow us to create a rigorous procedure for the generation of canonical SMILES which can be used for accurate and fast structural searching in large chemical databases.
Risk perception related to COVID-19 among the Iranian general population: An application of the extended parallel process model
Authors: Jahangiry Leila , Bakhtari Fatemeh , Sohrabi Zahara , Reihani Parvin , Samei Sirous , Ponnet Koen , Montazeri Ali 
Published: August 2020

Abstract
Background: The novel coronavirus disease 2019 (COVID-19) has emerged as a major global public health challenge. This study aimed to investigate how people perceived the COVID-19 outbreak using the components of the Extended Parallel Process Model (EPPM) and to find out how this might contribute to possible behavioral responses to the prevention and control of the disease.Methods: This cross-sectional study was conducted online in Iran in March and April 2020. The Data were collected using an electronic questionnaire via Porsline. Participants were recruited using online applications and posts on a number of platforms such as Telegram, WhatsApp, and Instagram asking people to take part in the study. To collect data a self-designed questionnaire based on the EPPM was used in order to measure the risk perception (efficacy, defensive responses, perceived treat) related to the COVID-19. Descriptive statics, chi-square, t-test and analysis of variance (ANOVA), were used to explore the data.Results: A total of 3,727 individuals with a mean age (SD) of 37.0 (11.1) years participated in the study. The results revealed significant progressive increases in efficacy, defensive responses and perceived treat particularly among those aged 60 and over. Women had significantly higher scores than men on some aspects such as self-efficacy, reactance, and avoidance but men had higher perceived susceptibility scores for COVID-19 than women. Overall 56.4% of participants were engaged in danger control (preventive behavior) while the remaining 43.6% were engaged in fear control (non-preventive behavior) process.Conclusion: More than half of all participants motivated by danger control. This indicated that more than half of participants had high perceived efficacy (i.e., self-efficacy and response efficacy). Self-efficacy scores were significantly higher among participants who were older, female, single, lived in rural areas, and had good economic status. The results suggest that socioeconomic and demographic factors are the main determinants of risk perceptions for controlling the COVID-19 pandemic.
Development and testing of a 3D-printable polylactic acid device to optimize a water bioremediation process
Authors: Marconi Patricia Laura , Trentini Andrea , Zawoznik Myriam , Nadra Carlos , Mercadé Juan Manuel , Novoa Juan Sanchez , Orozco Daniel , Groppa María Daniela 
Published: August 2020

Abstract
In the present work, a remediation bioprocess based on the use of a native isolate of Chlorella vulgaris immobilized in an alginate matrix inside a polylactic acid (PLA) device is proposed. This microalga immobilized in alginate beads was previously shown to be useful for the reduction of several chemical and microbial contaminants present in the highly polluted water from the Matanza-Riachuelo watershed. However, these beads had a relatively short shelf life in the natural environment. To overcome this limitation, a 3D-printed PLA device was designed. PLA is a biocompatible and biodegradable material suitable for biotechnological applications. We used Erlenmeyers and stirred-tank bioreactors fed batch with Murashige Skoog (MS) culture medium or water from the Cildáñez stream (one of the water bodies of the aforementioned watershed) to estimate the growth kinetics parameters and the bioremediation capacity of immobilized-microalgal cells as an unconfined system (UcS) or a confined system (CfS) inside PLA devices on Cildáñez water. Although alga’s growth parameters were maximum in the UcS fed with MS medium as substrate, successful bioremediation of the target water was possible using the CfS: all inorganic nitrogen forms and total phosphorus were reduced at least by 90% after 5 days of bioprocess in an agitated bioreactor, whereas aerobic mesophilic bacteria decreased by about 85%. The number of coliforms also decreased. Standardized cytotoxicity tests using Allium cepa seeds carried out to prove the effectiveness of the bioremediation process, confirmed the high degree of decontamination achieved by the use of immobilized microalga confined in a 3D-printable PLA-device.
Impact of retrieved lymph nodes count on short-term complications in patients with gastric cancer
Authors: Sun Feng , Liu Song , Song Peng , Zhang Chen , Liu Zhijian , Guan Wenxian , Wang Meng 
Published: August 2020

Abstract
Background: It is well established that retrieved lymph nodes (RLNs) count were positively correlated with better overall survival in gastric cancer (GC). But little is known about the relationship between RLNs count and short-term complications after radical surgery. Methods: A total of 1487 consecutive GC patients between January 2016 and December 2018 at Nanjing Drum Tower Hospital were retrospectively analyzed. Univariate analyses were performed to elucidate the association between RLNs count and postoperative complications. We further identified clinical factors that might affect the RLNs count. Results: Among all of the patients, postoperative complications occurred in 435 (29.3%) patients. The mean RLNs count was 25.1 and 864 (58.1%) patients were diagnosed with lymph node metastasis. Univariate analyses showed no significant difference between RLNs count and postoperative complications (both overall and stratified by CDC grade). Multivariate analyses further revealed that type of resection, tumor invasion, and lymph node metastasis were associated with RLNs count. Conclusions: The current study demonstrated that RLNs count was not associated with postoperative short-term complications following gastrectomy of GC, which provided a rationale for the determination of a proper RLNs count of curative gastrectomy.
Talking the talk in junior interprofessional education: Is healthcare terminology a barrier or facilitator?
Authors: Nadarajah Shamara , Azim Arden , Yılmaz Derya Uzelli , Sibbald Matthew 
Published: August 2020

Abstract
Background: Use of jargon and complex healthcare terminology is a potential barrier to interprofessional education (IPE). Healthcare terminology can be separated into two categories: inclusive terminology shared amongst professions, and exclusive terminology unique to one profession. We sought to understand how terminology is perceived by junior learners in an IPE setting.Methods: We conducted a mixed methods study involving medical, nursing, and physician assistant students attending IPE simulation workshops. Students reviewed scenarios used in the workshops and identified terminology they considered “inclusive” or “exclusive”. Then, students participated in focus group discussions surrounding attitudes/perceptions towards healthcare terminology.Results: 23 students analyzed 14 cases, identifying on average 21 terms per case as healthcare terminology (29% of overall word count). Of the 290 terms identified, 113 terms were classified as healthcare terminology, 46 as inclusive and 17 as exclusive by >50% of participants. Analysis of focus group transcripts revealed 4 themes: abbreviations were commonly perceived as complex terminology, unfamiliarity with terminology was often attributed to being early in training even if exclusive, simulation was considered a safe space for learning, and learning terminology was a valued objective in early IPE.Conclusions: Students perceive a lot of healthcare terminology in learning materials, which is recognized as a valuable learning objective in their early IPE experiences, but also a challenge. Categorization of healthcare language is inconsistent among students and may reflect individual differences in prior experiences. Overall, healthcare terminology is a valued desirable difficulty among junior learners, and should not be avoided in IPE.
Development of Competency Model for Family Physicians Against the Background of ‘Internet Plus Healthcare’in China：a Mixed Methods Study
Authors: Ni Ziling , Wang Xiaohe , Zhou Siyu , Zhang Tao 
Published: August 2020

Abstract
Background: Identification of the service competences of family physicians are central to ensuring high-quality primary care and improving patient outcomes. However, little is known about how to assess the family physicians’ service competences in primary care settings.It is necessary to develop and validate a general model of core competences of the family physician under the stage of construction of family doctor system and implementation of ‘Internet Plus Healthcare’ service model in China. Methods: The literature review, behavioral event interviews, expert consultation and questionnaire survey were performed. The scale’s 35 questions were measured by response rate, highest score, lowest score, and average score for each. Delphi method was used to assess content validity, Cronbach’s α to estimate reliability, and factor analysis to test structural validity. Respondents were randomly divided into two groups; data for one group were used for exploratory factor analysis (EFA) to explore possible model structure. Confirmatory factor analysis (CFA) was then performed. Results: Effective response rate was 93.56%. Cronbach’s α coefficient of the scale was 0.977. Factor analysis showed KMO of 0.988. Bartlett’s test showed χ2 of 22917.515 (df=630), p<.001. Overall authority grade of expert consultation was 0.80, and Kendall’s coefficient of concordance W was 0.194. By EFA, the five-factor model was retained after thorough consideration, and four items with factor loading less than 0.4 were proposed to obtain a five-dimension, 32-item scale. CFA was performed on the new structure, showing high goodness-of-fit test (NFI=0.98, TLI=0.91, SRMSR=0.05, RMSEA=0.04). Overall Cronbach’s α coefficients of the scale and each sub-item were greater than 0.9. Conclusions: The scale has good reliability, validity, and credibility, and can therefore serve as an effective tool for assessment of Chinese family physicians’ service competences.
Volumetric MRI analysis of brain structures in patients with history of first and repeated suicide attempts: a cross sectional study
Authors: Sarkinaite Milda , Gleizniene Rymante , Adomaitiene Virginija , Dambrauskiene Kristina , Raskauskiene Nijole , Steibliene Vesta 
Published: August 2020

Abstract
Background

Structural brain changes are found in suicide attempters, as well as in patients with mental disorders. It remains unclear whether the suicidal behavior is related to atrophy of brain regions and how the morphology of specific brain areas is changing with each suicide attempt. This cross-sectional study examined volumetric differences in brain regions among patients with history of first and repeated suicide attempts in comparison to healthy controls (HC).

Methods

The sample consisted of 56 adults, non-psychotic patients without cognitive impairment and any organic brain disorders hospitalized after first suicide attempt (first SA) (n=29) and more than one suicide attempt (SA>1) during the lifetime (n=27); and 54 adult volunteers without history of mental disorder and suicide attempts, designated as HC. The MRI data were collected using 1.5 T Siemens Avanto scanner. Brain cortical thickness, grey and white matter volumes were measured using FreeSurfer 6.0 automatic segmentation technique.

Results

In comparison to HC, patients with first SA had 3.5, 3.58 and 4.19% significantly lower mean cortical thickness of the superior and rostral middle frontal areas of the left hemisphere and superior frontal area of the right hemisphere, respectively; 4.09, 4.02 and 4.49% lower mean cortical thickness of the inferior, middle and superior temporal areas of the left hemisphere, respectively. In comparison to HC, patients after SA>1 had a significantly lower mean cortical thickness (from 4.02 to 8.33%) in ten areas of frontal cortex of the left hemisphere and seven areas of the right hemisphere; from 3.90 to 6.04% difference in six areas of temporal cortex in both hemispheres. The comparison of hippocampus volume showed a significantly lower mean volume (7.86 to 9.89%) of left and right parts in patients with SA>1, but not in patients with first SA.

Conclusions

Hospitalized suicide attempters had lower frontal and temporal cortical thickness and smaller parts of hippocampus than HC; these differences were significantly higher in repeated suicide attempters than in patients with first SA. Our findings suggest that repeated suicidal behavior is associated with intensifying atrophy of specific brain structures, independently of diagnosis of depressive disorders.
Genotype classification and fluorescence visual identification of the Rhizoma Paridis of Paris polyphylla&nbsp;var. yunnanensis based on the SNPs of ITS sequence&nbsp;
Authors: Gao Chi , Zhang Kaiyuan , Wang Qinghe , Zhao Ling , Chen Rong , Yin Hongxiang 
Published: August 2020

Abstract
A fluorescent visual identification system of Paris polyphylla var. yunnanensis was established based on internal transcribed spacer barcoding. It is proposed for the first time that P. polyphylla var. yunnanensis should be divided into two types of genotypes: YN-I and YN-II according to single nucleotide polymorphism of internal transcribed spacer. In order to avoid false-negative results, two pairs of specific primers for YN-I and YN-II were designed, respectively, and specific visual fluorescent identification systems was established by SYBR Green I fluorescent dye was directly introduced into the PCR system which can be observed directly with the naked eye for the green fluorescent color of PCR system. Therefrom, it has realized the rapid and directly visual identification of two genotypes of P. polyphylla var. yunnanensis from its common nine adulterants. This study proposed for the first time the existence of different genotypes on the legal basis of Rhizoma Paridis, and provided a model for the accurate identification of different genotypes.