bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of immune-responsive gene 1 prevents acute otitis media due to streptococcus pneumoniae infection through Nrf2- Hmox1 pathway
Authors: Yang Peizhen , Li Yufen , Wang Dongqing , Chen Zhipeng 
Published: December 2019

Abstract
Background: Acute otitis media (AOM) is one of the most commonly diagnosed childhood diseases that are associated with influenza infection. Immunoresponsive gene 1 (Irg1) is overexpressed in mammalian macrophage during inflammation process. Here, the present study aims to investigate the role of Irg1 in streptococcus pneumoniae infection-induced inflammatory response in AOM through Nrf2 signaling pathway. Methods: Positive rate of streptococcus pneumoniae and expression of Irg1, Nrf2 and Hmox1 were determined in the middle ear lavage fluid from 85 AOM patients infected with streptococcus pneumoniae and 48 healthy subjects. After the successful establishment of mice model with AOM, macrophage was harvest from the middle ear lavage fluid of mice. At last, to investigate the effect of Irg1 and Nrf2 signaling pathway on inflammatory response and streptococcus pneumoniae infection by accumulating macrophage, Irg1, sh-Irg1 and retinoic acid (an inhibitor of Nrf2 signaling pathway) were injected into AOM mice. Results: Irg1 exhibited a high level and activated Nrf2 signaling pathway was detected in AOM. Besides, positive rate of streptococcus pneumoniae was increased in AOM. Furthermore, in the mice model with AOM, Irg1 could repress inflammatory response by downregulating expression of TNF-α, IL-6 and IL-1β and inhibit streptococcus pneumoniae infection by stimulating accumulation of macrophage to increase its endocytosis through activation of Nrf2 signaling pathway. Conclusion: Taken together, Irg1 contributes to inhibiting inflammatory response and streptococcus pneumoniae infection in AOM by promoting accumulation of macrophage through activation of Nrf2 signaling pathway, which provides novel therapeutic targets for AOM therapy.
Peri-treatment peripheral blood cell score predicts long-term locoregional progression hazard in oesophageal squamous cell carcinoma after definitive chemoradiotherapy
Authors: Chen Jianzhou , Xu Liangyu , Guo Hong , Huang Ruihong , Guo Longjia , Yu Yuanxiang , Zhai Tiantian , Wu Fangcai , Chen Zhijian , Li Derui , Chen ChuangZhen 
Published: December 2019

Abstract
Background Prediction of response to chemoradiotherapy is critical for the optimal management of oesophageal cancer, yet it is still an unmet clinical need. This study aims to evaluate the predictive potential of peri-treatment peripheral blood cells (PBC) in disease progression hazard in oesophageal cancer following chemoradiotherapy.Methods 87 patients with primary oesophageal squamous cell carcinoma were subjected to definitive concurrent chemoradiotherapy in a phase II trial. PBC parameters (haemoglobin, neutrophils, platelets, lymphocytes and monocytes) were collected at 7 time points through the course of radiotherapy. The values of peri-treatment PBC parameters in predicting 3-year cumulative hazard of tumour progression were evaluated.Results Patients with disease progression displayed distinct distribution patterns of peri-treatment PBC compared to patients without. Greater prediction capabilities for risk of locoregional disease progression were found in PBC collected after the start of radiotherapy compared to their pretreatment counterparts, and in individual parameters rather than cell-to-cell ratios. The most predictive PBC parameters were integrated by summation and designated as a PBC score (PBCS), which further augmented their predictive power. Patients divided according to their PBCS (high vs medium vs low) had significantly different 3-year cumulative hazards of locoregional progression (58% vs 29% vs 7%, P = 0.0017). Multivariate analysis confirmed that PBCS high (HR 12.2, 95%CI 2.0-76.3, P = 0.007) and medium (HR 5.8, 95%CI 1.2-27.7, P = 0.028) are independent indicators of locoregional progression.Conclusion Peri-treatment PBCS can predict the long-term hazard of locoregional progression after definitive chemoradiotherapy in patients with oesophageal squamous cell carcinoma.
Catalytic hydrolysis of Dichlorodifluoromethane over MoO3/ZrO2 -TiO2 solid acid
Authors: Li Zhiqian , Zhou Tong , Ren Guoqing , Tan Xiaofang , Jia Lijuan , Liu Tiancheng 
Published: December 2019

Abstract
The catalytic behaviors of solid acid of MoO3/ZrO2-TiO2 calcined at different temperature for the catalytic hydrolysis of Dichlorodifluoromethane have been studided. The effects of catalytic hydrolysis temperature and water vapor concentration on catalytic hydrolysis of Dichlorodifluoromethane were also studied. The Results show 98.65 % of Dichlorodifluoromethane is degraded over MoO3/ZrO2-TiO2 catalyst calcined at 500 ℃ with a concentration of water vapor of 83.18% when the hydrolysis temperature is 400。C and the Dichlorodifluoromethane flux rate is 1mL/min with main degradation products were CO, CO2, HF and HCl. A maintained degradation rate of 65.34 % of Difluoromethylene Chloride has been observed through 30 hours’ continuous reaction over the catalyst of MoO3/ZrO2-TiO2. The XRD result reveals the main phase of solid MoO3/ZrO2-TiO2 catalyst is the tetragonal Zr(MoO4)2 that doped TiO2 of anatase.
Association of Chinese sauerkraut consumption with esophageal cancer in Chinese populations: A meta-analysis
Authors: Han Dan , Liu Shuang , Liang Kaige , huang yaojiang 
Published: December 2019

Abstract
Background With accumulating evidence showing that Chinese sauerkraut consumption may be associated with the developmental of Esophageal cancer.Objective We carried out a analysis by R to Evaluate whether the consumption of Chinese sauerkraut is associated with Esophageal cancer in Chinese populations.Methods Two independent investigators carry out a systematic search through Oct 8, 2019 for all studies. The search terms included Chinese sauerkraut, Chinese pickled vegetable, and Suancai, in combination with Esophageal cancer. Investigators extracted, pooled and analysed data from the included studies using a random-effects model in R.Results A random-effects meta-analysis of all 23 studies, including 32,259 unique participants, indicated that Chinese sauerkraut consumption is associated with a significantly increased risk of Esophageal cancer compared to controls (OR=1.81, 95% CI=1.50-2.19, P=0.00001). Sensitivity analysis showed that no single study significantly influenced the overall association. Most of the subgroup analyses, including those of subtype of EC, geographic area, publication language and year, demonstrated a statistically significant association between consuming Chinese sauerkraut and Esophageal cancer risk. Meta-regression indicated that gender and publication year is positively correlated with effect sizes. Furthermore, the trim-and-fill method used to adjust for funnel plot asymmetry in our meta-analysis confirmed that a positive outcome is unlikely to be due to publication bias.Conclusion The results of this meta-analysis provide evidence that consuming Chinese sauerkraut may increase the risk of Esophageal cancer.
Diagnostic performance of nucleic acid tests in tuberculous pleurisy
Authors: Xiao He-ping , Yan Liping , Han Min 
Published: December 2019

Abstract
Background: Tuberculous pleurisy (TBP) is the most common form of extrapulmonary tuberculosis (TB). However, rapid diagnostic methods with high accuracy for tuberculous pleurisy are urgently needed. In the present study, we evaluated the diagnostic accuracy of Xpert MTB/RIF, LAMP and SAT-TB assay with pleural fluids from from culture-positive TBP patients. Methods: We prospectively enrolled 300 patients with exudative pleural effusions used as the samples for Xpert MTB/RIF, LAMP and SAT-TB assay. Of these, 265 including 223 patients diagnosed with TP and 42 non-TB patients used as controls were analyzed. Results: The sensitivities of Xpert MTB/RIF (27.4%) , LAMP (26.5%) and SAT-TB assay (32.3%) were significantly higher than that of pleural effusion smear (14.3% , X2 = 20.65, P < 0.001), whereas they were much lower than expected for the analysis of pleural effusion samples. Both SAT-TB assay and Xpert MTB/RIF demonstrated high specificities (100%) and PPVs (100%), but the NPVs of all of the tests were < 22%. The area under ROC curve of pleural effusion smear, LAMP, Xpert MTB/RIF and SAT-TB assays was 0.524 (95% CI 0.431–0.617), 0.632 (95% CI 0.553–0.71), 0.637 (95% CI 0.56–0.714) and 0.673 (95% CI 0.6–0.745). SAT-TB assays had the highest AUC. Conclusion: Nucleic acid amplification tests are not the first choice in the diagnosis of tuberculous pleurisy. In this type of test, SAT-TB is recommended because of its low cost, relatively more accurate compared with the other two tests. This prospective study was approved by The Ethics Committee of the Shanghai Pulmonary Hospital (approval number: K19-148). ClinicalTrials.gov identifier: ChiCTR1900026234.
Palliative nasogastric tube placement for patients with advanced esophageal cancer with nearly total obstruction: Consider the feasibility and acceptability considerations
Authors: YANG CHIH WEI , Huang I-Hsuan , Chang Wei-Kuo 
Published: December 2019

Abstract
Background: Nasogastric tube (NGT) feeding was reasonable choice for patients with advanced esophageal cancer with a short-life expectancy. Bedside blind NGT placement beyond the nearly total obstruction lesion usually fail and is challenging. Each individual patient might have different cancer stage, tumor location and size, natural course of the disease, technique feasibility, and tolerability of NGT placement. This study evaluates the benefits and limitations of palliative NGT placements for advanced esophageal cancer during their last months of life. Method: Retrospective observation study was performed. We implemented three techniques of palliative NGT placement, compared the advantages and limitations, and evaluated the clinical outcomes in patients advanced esophageal cancer with nearly total obstruction. The present study was performed in at a tertiary care teaching hospital, Taiwan. Patients (n =32) received palliative care, failure of bedside blind NGT placement, and/or NPO (Nil per os) treatment were included. Patients were divided into different palliative NGT placements: guidewire method (n = 6), the drag method (n = 6), push method (n = 10). Results: Success rate of palliative NGT placement was observed in the guidewire method (75%), drag method, (100%), and push method (93%). Compared the palliative NGT groups to NPO group, NGT groups had significantly increased in enteral caloric intake (p < 0.05), serum albumin level (p < 0.01), decreased the length of hospital stay (p = 0.01), but increased the survival time (p = 0.01). Conclusion: Patients who tolerated the NGT placement will able to receive desired caloric intake, decrease length of hospital stay, and increase the overall survival time.
Evidence for an ancient bilaterian origin of the RAG-like transposon
Authors: Martin Eliza C. , Vicari Celia , Tsakou-Ngouafo Louis , Pontarotti Pierre , Petrescu Andrei J. , Schatz David G. 
Published: December 2019

Abstract
Background V(D)J recombination is essential for adaptive immunity in jawed vertebrates and is initiated by the RAG1-RAG2 endonuclease. The RAG1 and RAG2 genes are thought to have evolved from a RAGL (RAG-like) transposon containing convergently-oriented RAG1-like (RAG1L) and RAG2-like (RAG2L) genes. Elements resembling this presumptive evolutionary precursor have thus far only been detected convincingly in deuterostomes, leading to the model that the RAGL transposon first appeared in an early deuterostome. Results We have identified numerous RAGL transposons in the genomes of protostomes, including oysters and mussels (phylum Mollusca) and a ribbon worm (phylum Nemertea), and in the genomes of several cnidarians. Phylogenetic analyses indicate that the RAGL transposon family evolved in a vertical manner within the Bilateria clade. Many of the RAGL transposons identified in protostomes are intact elements containing convergently oriented RAG1L and RAG2L genes flanked by terminal inverted repeats (TIRs) and target site duplications with striking similarities with the corresponding elements in deuterostomes. In addition, protostome genomes contain numerous intact RAG1L-RAG2L adjacent gene pairs that lack detectable flanking TIRs. Domains and critical active site and structural amino acids needed for endonuclease and transposase activity are present and conserved in many of the predicted RAG1L and RAG2L proteins encoded in protostome genomes. Conclusions Active RAGL transposons were present in multiple protostome lineages and were transmitted vertically during protostome evolution. It appears that the RAGL transposon family was broadly active during bilaterian evolution, undergoing multiple duplication and loss/fossilization events, with the RAGL genes that persist in present day protostomes perhaps constituting both active RAGL transposons and domesticated RAGL genes. Our findings indicate that the RAGL transposon arose earlier in evolution than previously thought, either in an early bilaterian or prior to the divergence of bilaterians and non-bilaterians, and alter our understanding of the evolutionary history of this important transposon family.
Remote Closed-Loop Automatic Oxygen Control in Preterm Infants
Authors: Moradi Mohammad Reza , Kalhori Sharareh R. Niakan , Saeedi Marian Ghazi , Zarkesh Mohammad Reza , Habibelahi Abbas , Panahi Amir Hossein 
Published: December 2019

Abstract
Background Different automated systems have been developed to improve maintenance of target range of arterial oxygen saturation (SPO2) in premature infants with respiratory distress. This study aimed to develop a Remote Closed-Loop Automatic Oxygen Control (RCLAC) as an efficient monitoring device. Then the mean of fraction of inspired oxygen (FIO2) and SPO2 by routine manual control (RMC) and RCLAC were compared.Methods A developmental-descriptive study was carried out in an Iranian hospital (Tehran-Iran; 2015-2017). Eighteen preterm infants with gestational age 24-28 weeks entered the study. A database was prepared based on pulse oximeter parameters. A Wi-Fi module was implemented to receive data from pulse oximeter and send inputs to user's mobile. Vibrate alarm was implemented for high or low FIO2. After receiving notifications associated with increase or decrease of FIO2 levels and user's confirmation; the alterations were applied on the ventilator.Results The mean FIO2 in RMC system was significantly higher than RCLAC system (98.1± 2.67 vs. 79.5±16.03; p = 0.0001). According to the results, when the SPO2 reached close to target SPO2 range and consequently FIO2 changed (decreased or increased based on target SPO2), heart rate showed a regular beating with decrease in the numbers.Conclusion Remote Closed-Loop Automatic Oxygen Control system as a simple device could prevent preterm neonates from sustained hypo-hyperoxemic and arrhythmia episodes. Moreover by using RCLAC, there was no need for continuous monitoring that may reduce workload of NICUs medical staff. Collecting reliable data and recording information in digital forms were also other benefits.
Femoral periosteal reaction in the mid-piece of the prosthesis: a potential predictor of hip prosthetic joint infection
Authors: Zhang Zijie , Huang Zida , Li Wenbo , Fang Xinyu , Zhang Wenming 
Published: December 2019

Abstract
[Background] Early diagnosis of HPJI is important. Periosteal reaction has been shown to diagnose osteomyelitis and bone tumours; however, research on artificial joint infections is not clear. The purpose of this study was to analyse the diagnostic possibility of FPR on X-ray films for HPJI. [Methods] From January 2017 to June 2019, 105 patients who underwent artificial hip joint revision surgery were retrospectively included in the single joint surgery centre. Based on the description by the MSIS in 2013 and exclusion of patients with bone tumour revisions, 66 patients were reviewed and divided into the PJI group and aseptic failure (AF) group. The clinical outcome and radiographic results were examined by two experienced joint surgeons independently on hip X-ray images in all cases. Sensitivity, specificity, PPV and NPV were calculated for FPR in HPJI, and the imaging results were compared with those of serology and bacterial culture. [Results] A total of 16 patients in the PJI group were positive for FPR with appearance in the mid-piece and distal part, while 6 patients in the AF group (43.3% vs 26.1%, p = 0.04) were positive for FPR. Only 1 patient in the AF group had a mid-piece FPR, and the rest were at the distal end. Separated measurements were unidimensional (Kappa value = 0.89). The process of FPR-positive patients in the PJI group was 13 ± 26.1 months, and it was 9.83 ± 8.2 months in the AF group, and the difference was not statistically significant (p = 0.67). There was no connection between the responsible pathogens and FPR in the PJI group. The sensitivity, specificity, PPV and NPV for FPR at any section were 43.2%, 79.3%, 72.7% and 52.7%, respectively. The mid-piece FPR improved by 43.2%, 96.5%, 94.1% and 57.1%, respectively. Combined with elevated serum CRP/ ESR, specificity and PPV could reach 100%. [Conclusion] Preoperative X-ray examinations revealed that the mid-piece FPR requires a high degree of suspicion of PJI. If patients have elevated serum CRP/ESR at the same time, joint puncture and other methods should be used to obtain culture results to confirm the diagnosis.
Comparison between the Effects of Pre- and Postoperative Myocardial Injuries on 30-Day Mortality After Non-Cardiac Surgery: A Retrospective Analysis Using an Inverse Probability Weighting Adjustment
Authors: Lee Seung-Hwa , Park Jungchan , Lee Jong-Hwan , Min Jeong Jin , Hong Kwan Young , Cho Hyojin , Carriere Keumhee , Ahn Joonghyun 
Published: December 2019

Abstract
Background: Although both pre- and postoperative myocardial injuries are strongly associated with an increased postoperative mortality, no study has directly compared the effects of pre- and postoperative myocardial injuries on 30-day mortality after non-cardiac surgery. Therefore, we evaluated and compared the effects of pre- and postoperative myocardial injury on 30-day mortality after non-cardiac surgery.Methods: From January 2010 to December 2016, patients undergoing non-cardiac surgery were stratified into either the normal (n = 3,182), preoperative myocardial injury (n = 694), or postoperative myocardial injury (n = 756) groups. Myocardial injury was defined as a sole elevation of cardiac troponin value above the 99th percentile upper reference limit without ischemic symptom using the 4th universal definition of myocardial infarction. Patients in the preoperative myocardial injury group were further divided into the attenuated (n = 177) or persistent myocardial injury group (n = 517) according to the changes in postoperative cardiac troponin level. As the primary outcome, postoperative 30-day mortalities were compared among the groups using the weighted Cox proportional-hazards regression models with the inverse probability weighting.Results: Compared with the normal group, postoperative 30-day mortality was increased significantly both in the pre- and postoperative myocardial injury groups (1.4% vs. 10.7%; hazard ratio [HR] 3.12; 95% confidence interval [CI] 1.62-6.01; p = 0.001 and 1.4% vs. 7.4%; HR 4.49; 95% CI 2.34-8.60; p < 0.001, respectively), however, there was no difference between the pre- and postoperative myocardial injury groups (HR, 1.44; 95% CI 0.79–2.64; p = 0.45). In addition, the attenuated myocardial injury group showed a significantly lower postoperative 30-day mortality than the persistent myocardial injury group (5.6% vs. 12.4%; HR 2.23; 95% CI 1.17–4.44; p = 0.02).Conclusion: In patients undergoing non-cardiac surgery, preoperative myocardial injury also increased postoperative 30-day mortality to a similar degree of postoperative myocardial injury. In addition, attenuation of preoperative myocardial injury might decrease in 30-day mortality after non-cardiac surgery.
Interventions for prudent antibiotic use in primary healthcare: An econometric analysis
Authors: Carlsson Fredrik , Lampi Elina , Sundvall Pär-Daniel , Torres Marcela Jaime , Ulleryd Peter , Åhrén Christina , Jacobsson Gunnar 
Published: December 2019

Abstract
Background Rational antibiotic prescribing is crucial to combat antibiotic resistance. Optimal strategies to improve antibiotic use are not known. Strama, the Swedish strategic program against antibiotic resistance, has been successful in reducing antibiotic prescription rates. This study investigates whether two specific interventions directed toward healthcare centers, an informational visit and a self-evaluation meeting, played a role in observed reduction in rates of antibiotic prescriptions in primary healthcare.Methods The study was a retrospective, observational, empirical analysis exploiting the variation in the timing of the interventions and considering past prescriptions through use of estimations from dynamic panel data models. Primary healthcare data from 2011 to 2014 were examined. Data were from public and private primary healthcare centers in western Sweden. The key variables were prescription of antibiotics and indicator variables for the two interventions.Results The first intervention, an educational information intervention, decreased the number of prescriptions among public healthcare centers, but this effect was only temporary. We found no proof that the second intervention, a self-evaluation meeting at the healthcare center, had an impact on the reduction of prescriptions.Conclusions Single educational interventions aimed at influencing rates of antibiotic prescriptions have limited impact. A multifaceted approach is needed in efforts to reduce the use of antibiotics in primary health care.
WITHDRAWN: The Male Reproductive Structure and Spermatogenesis of Trypophloeus Klimeschi Eggers (Coleoptera: Curculionidae: Scolytinae)
Authors: Gao Jing , Gao Guanqun , Dai Lulu , Wang Jiaxing , Chen Hui 
Published: December 2019

Abstract
Background Trypophloeus Klimeschi Eggers (Coleoptera: Curculionidae: Scolytinae) is one of the most destructive pests of Populus alba var. pyramidalis (Bunge), resulting in significant losses in economic, ecological and social benefits in China’s northwest shelter forest. But research of reproductive system, spermiogenesis and spermatozoon ultrastructure of T. klimeschi that is basis of phylogeny, reproductive biology and controlling is still black. Results The male reproductive organ of T. klimeschi is composed of testis, seminal vesicle, strand shaped accessory gland containing long branch of strand shaped accessory gland and short branch of strand shaped accessory gland, curly accessory gland, vas deferens and a common ejaculatory duct. The number of sperm per cyst is 350~512. Its spermatozoon is slender, measuring about 75 μm in length and 0.5 μm in wide and composed of a 3-layred acrosomal complex, a nucleus with two different states of aggregation, two mitochondrial derivatives with dark crystal, a 9+9+2 axoneme that run more or less parallel to mitochondrial derivatives, two crystalline accessory bodies with a big compact “puff”-like expansion. Especially in the seminal vesicle, its long flagella folded into several turns and the whole sperm is wrapped in a film.Conclusion The general morphology of male reproductive tract, the spermatogenesis and the spermatozoa of T. klimeschi are, for the most part, similar to the majority of the Curculionidae. However, some distinct differences were found: the low electron-dense band in the cytoplasm of spermatocytes; two different aggregation states of spermatozoon nucleus; especially the stored way of T. klimeschi spermatozoa.