bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of potential plant-pollinator network structure for 3 seed mixes (lawn, roadside, and pollinator-planting) in western NY, USA
Authors: Burt Briana , Chomiak Kristina , Cisse Ibrahim , Paratore Aaron , Whitney Kaitlin Stack 
Published: October 2020

AbstractThere is growing concern, locally and globally, about the health of pollinating insects and their decreasing abundance and diversity. While roads may also be contributing to insect pollinator declines (roads can contribute to habitat fragmentation and habitat destruction), roadsides may provide opportunities for pollinating insect conservation. Yet to use these areas to support local pollinating insects, we need to understand which plants will support wild pollinators, especially of conservation concern. To that end, we researched the potential plant-pollinator networks of three existing seed mixes in western New York (USA) – a roadside seed mix, a pollinator-friendly planting mix, and a lawn seed mix. We used publicly available information and built bipartite graphs to show the resulting networks. The pollinator-friendly seed mix supported the most pollinating insects overall and taxa of conservation concern. Yet the roadside mix, with the same species richness as the lawn seed mix, supported a different network based on the plants in the mix. Our results inform which particular plant species in existing seed mixes in western New York can support wild pollinating insect species of concern in the region. Additionally, our results show potentially how roadside and lawn plantings may be altered to support a broader network of pollinating insects.
Synthesis and preclinical evaluation of [11C]MTP38 as a novel PET ligand for phosphodiesterase 7 in the brain
Authors: Obokata Naoyuki , Seki Chie , Hirata Takeshi , Maeda Jun , Ishii Hideki , Nagai Yuji , Matsumura Takehiko , Takakuwa Misae , Fukuda Hajime , Minamimoto Takafumi , Kawamura Kazunori , Zhang Ming-Rong , Nakajima Tatsuo , Saijo Takeaki , Higuchi Makoto 
Published: October 2020

AbstractPurposePhosphodiesterase (PDE) 7 is a potential therapeutic target for neurological and inflammatory diseases, althoughin-vivovisualization of PDE7 has not been successful. In this study, we aimed to develop [11C]MTP38 as a novel positron emission tomography (PET) ligand for PDE7.Methods[11C]MTP38 was radiosynthesized by11C-cyanation of a bromo precursor with [11C]HCN. PET scans of rat and rhesus monkey brains andin-vitroautoradiography of brain sections derived from these species were conducted with [11C]MTP38. In monkeys, dynamic PET data were analyzed with an arterial input function to calculate the total distribution volume (VT). The non-displaceable binding potential (BPND) in the striatum was also determined by a reference tissue model with cerebellar reference. Finally, striatal occupancy of PDE7 by an inhibitor was calculated in monkeys according to changes inBPND.Results[11C]MTP38 was synthesized with radiochemical purity ≥ 99.4% and molar activity of 38.6 ± 12.6 GBq/μmol. Autoradiography revealed high radioactivity in the striatum and its reduction by non-radiolabeled ligands, in contrast with unaltered autoradiographic signals in other regions.In-vivoPET after radioligand injection to rats and monkeys demonstrated that radioactivity was rapidly distributed to the brain and intensely accumulated in the striatum relative to the cerebellum. Correspondingly, estimatedVTvalues in the monkey striatum and cerebellum were 3.59 and 2.69 mL/cm3, respectively. The cerebellarVTvalue was unchanged by pretreatment with unlabeled MTP38. StriatalBPNDwas reduced in a dose-dependent manner after pretreatment with MTP-X, a PDE7 inhibitor. Relationships between PDE7 occupancy by MTP-X and plasma MTP-X concentration could be described by Hill’s sigmoidal function.ConclusionWe have provided the first successful preclinical demonstration ofin-vivoPDE7 imaging with a specific PET radioligand. [11C]MTP38 is a feasible radioligand for evaluating PDE7 in the brain and is currently being applied to a first-in-human PET study.
Neuroimaging: into the Multiverse
Authors: Dafflon Jessica , Da Costa Pedro F. , Váša František , Monti Ricardo Pio , Bzdok Danilo , Hellyer Peter J. , Turkheimer Federico , Smallwood Jonathan , Jones Emily , Leech Robert 
Published: October 2020

AbstractFor most neuroimaging questions the huge range of possible analytic choices leads to the possibility that conclusions from any single analytic approach may be misleading. Examples of possible choices include the motion regression approach used and smoothing and threshold factors applied during the processing pipeline. Although it is possible to perform a multiverse analysis that evaluates all possible analytic choices, this can be computationally challenging and repeated sequential analyses on the same data can compromise inferential and predictive power. Here, we establish how active learning on a low-dimensional space that captures the inter-relationships between analysis approaches can be used to efficiently approximate the whole multiverse of analyses. This approach balances the benefits of a multiverse analysis without the accompanying cost to statistical power, computational power and the integrity of inferences. We illustrate this approach with a functional MRI dataset of functional connectivity across adolescence, demonstrating how a multiverse of graph theoretic and simple pre-processing steps can be efficiently navigated using active learning. Our study shows how this approach can identify the subset of analysis techniques (i.e., pipelines) which are best able to predict participants’ ages, as well as allowing the performance of different approaches to be quantified.
Absolute measurement of cellular activities using photochromic single-fluorophore biosensors
Authors: Gielen Vincent , Mönkemöller Viola , Bierbuesse Franziska , Bourges Anaïs C. , Vandenberg Wim , Shen Yi , Hofkens Johan , Vanden Berghe Pieter , Campbell Robert E. , Moeyaert Benjamien , Dedecker Peter 
Published: October 2020

AbstractGenetically-encoded biosensors based on a single fluorescent protein are widely used to visualize analyte levels or enzymatic activities in cells, though usually to monitor relative changes rather than absolute values. We report photochromism-enabled absolute quantification (PEAQ) biosensing, a method that leverages the photochromic properties of biosensors to provide an absolute measure of the analyte concentration or activity. We develop proof-of-concept photochromic variants of the popular GCaMP family of Ca2+biosensors, and show that these can be used to resolve dynamic changes in the absolute Ca2+concentration in live cells. We also show how our method can be expanded to fast imaging with reduced illumination intensities or to situations where the absolute illumination intensities are unknown. In principle, PEAQ biosensing can be applied to other biosensors with photochromic properties, thereby expanding the possibilities for fully quantitative measurements in complex and dynamic systems.
The evolution of hematopoietic cells under cancer therapy
Authors: Pich Oriol , Cortes-Bullich Albert , Muiños Ferran , Pratcorona Marta , Gonzalez-Perez Abel , Lopez-Bigas Nuria 
Published: October 2020

AbstractChemotherapies may influence the evolution of somatic tissues through the introduction of genetic variation in cells and by changing the selective pressures they face. However, the contributions of chemotherapeutic agents to the mutation burden of healthy cells and to clonal expansions in somatic tissues are not clear. Here, we exploit the mutational footprint of some chemotherapies to explore their influence on the evolution of hematopoietic cells. Cells of Acute Myeloid Leukemia (AML) secondary to treatment with platinum-based drugs showed a clear mutational footprint of these drugs, indicating that healthy blood cells received chemotherapy mutations. In contrast, no trace of 5-fluorouracil (5-FU) mutational signature was found in AML secondary to exposure to 5-FU, suggesting that cells establishing the AML were quiescent during treatment. We used the platinum-based mutational signature as a barcode to precisely time clonal expansions with respect to the moment of exposure to the drug. The enrichment for clonal mutations among treatment-related mutations in all platinum-treated AMLs shows that these secondary neoplasms begin their clonal expansion after the start of the cytotoxic treatment. In contrast, the absence of detectable platinum-related mutations in healthy blood samples with clonal hematopoiesis is consistent with a clonal expansion that predates the exposure to the cytotoxic agent, which favours particular pre-existing clones.
Saracatinib is an efficacious clinical candidate for fibrodysplasia ossificans progressiva
Authors: Williams Eleanor , Bagarova Jana , Kerr Georgina , Xia Dong-Dong , Place Elsie S. , Dey Devaveena , Shen Yue , Bocobo Geoffrey A. , Mohedas Agustin H. , Huang Xiuli , Sanderson Philip E. , Lee Arthur , Zheng Wei , Economides Aris N. , Smith James C. , Yu Paul B. , Bullock Alex N. 
Published: October 2020

AbstractCurrently, no effective therapies exist for fibrodysplasia ossificans progressiva (FOP), a rare congenital syndrome in which heterotopic bone is formed in soft tissues due to dysregulated activity of the bone morphogenetic protein (BMP) receptor kinase ALK2/ACVR1. From a screen of known biologically active compounds, we identified saracatinib as a potent ALK2 kinase inhibitor. In enzymatic and cell-based assays, saracatinib preferentially inhibited ALK2 compared with other receptors of the BMP/TGFβ signaling pathway, and induced dorsalization in zebrafish embryos consistent with BMP antagonism. We further tested the efficacy of saracatinib using an inducibleACVR1Q207Dtransgenic mouse line, which provides a model of heterotopic ossification, as well as an inducibleACVR1R206Hknock-in, which serves as a genetically and physiologically faithful model of FOP. In both models, saracatinib was well tolerated and potently inhibited the development of heterotopic ossification even when administered transiently following soft tissue injury. Together, these data suggest that saracatinib is an efficacious clinical candidate for repositioning in the treatment of FOP, offering an accelerated path to clinical proof of efficacy studies and potentially significant benefits to individuals with this devastating condition.
Identification of qPCR reference genes suitable for normalising gene expression in the developing mouse embryo
Authors: Hildyard John C.W. , Wells Dominic J. , Piercy Richard J. 
Published: October 2020

AbstractMammalian embryogenesis is an intricate, tightly orchestrated process. Progression from zygote through somitogenesis and on to organogenesis and maturity involves many interacting cell types and multiple differentiating cell lineages. Quantitative PCR analysis of gene expression in the developing embryo is a valuable tool for deciphering these interactions and tracing lineages, but normalisation of qPCR data to stably expressed reference genes is essential. Patterns of gene expression change globally and dramatically as embryonic development proceeds, rendering identification of appropriate reference genes challenging at both the whole embryo- and individual tissue-level. We have investigated expression stability in mouse embryos from mid to late gestation (E11.5–E18.5), both at the whole-embryo level, and within more restricted tissue domains (head, developing forelimb), using 15 candidate reference genes (ACTB, 18S, SDHA, GAPDH, HTATSF1, CDC40, RPL13A, CSNK2A2, AP3D1, HPRT1, CYC1, EIF4A, UBC, B2MandPAK1IP1), and four complementary algorithms (geNorm, Normfinder, Bestkeeper and deltaCt). Unexpectedly, all methods suggest that many genes within our candidate panel are acceptable references, and despite disagreement over highest-scoring candidates,AP3D1,RPL14AandPAK1IP1are the strongest performing genes overall. Conversely,HPRT1andB2Mare consistently poor choices: these genes show strong developmental regulation. We further show that use ofAP3D1,RPL13AandPAK1IP1can reveal subtle patterns of developmental expression even in genes ostensibly ranked as acceptable (CDC40,HTATSF1), and thus these three represent universally suitable reference genes for the mouse embryo.
Consumption of packaged foods by the Portuguese population: type of materials and its associated factors
Authors: Costa Sofia Almeida , Vilela Sofia , Correia Daniela , Severo Milton , Lopes Carla , Torres Duarte 
Published: October 2020

PurposeThis study aims to evaluate in the Portuguese population the consumption of packaged food (PF) vs non-packaged food, the associated factors and to estimate the contribution of specific food groups to the use of packaging materials.Design/methodology/approachA representative sample of the population was evaluated within the National Food, Nutrition and Physical Activity Survey 2015–2016 (n = 5,811, 3 months-84y). Dietary data were collected by two non-consecutive food diaries (children) or 24-h recalls, using a software program which integrates FoodEx2—Food classification system. Food packaging materials were measured according to the amount of PF by linear regression. The results were analyzed considering the distribution of the Portuguese population.FindingsThe reported amount of PF was 1,530 g/person/day (57%), in which PF in plastic was the most reported (69%), mainly associated with “Non-alcoholic beverages” (38%). “Fruit and vegetables” food group is most frequently reported without a package (35%). Men consumed significantly more quantity of PF for all materials, excepted for “paperboard/paper”, but also significantly more quantity of food without a package (β = 135.3 [95%IC: 63.7; 207.0]). Children and adolescents consumed more quantity of PF in multilayer materials (β = 177.8 [95%IC: 154.8; 200.9]) and significantly less quantity of food without a package (β = −343.8 [95%IC: −408.9; −278.6]). Also, more educated people consume more food without a package (β = 106.9 [95%IC: 33.2; 180.7]).Originality/valueThe Portuguese population presents a large consumption of PF. Male population and adults were identified as groups with higher consumption of PF.
The evolution of China's rural labor market in the 21st century: an empirical study based on nationally representative survey data at the household level
Authors: Zhang Tonglong , Huang Xiaowen , Zhang Lina , Zhang Linxiu 
Published: October 2020

PurposeThe purpose of this paper is to assess the development of China's rural labor markets and the identification of the important factors that affect rural labor's off-farm employment and migration.Design/methodology/approachBased on a set of long-term panel data, this paper makes a clear judgment on the trend of rural labor transfer. High-quality survey design makes it possible to examine the development of the rural labor market from multiple dimensions. Adding household fixed effects to the empirical model alleviates endogenous problems.FindingsThe authors find that the increasing trend toward off-farm employment, which is dominated by migration, has continued. There are some other important findings: (1). young male workers dominated off-farm employment, but the gap between groups continues to narrow; (2). the structure of employment is a good response to the economic transformation and (3). the quality of off-farm laborers, especially in terms of human capital, has also enhanced significantly and has continued to support off-farm employment and migration. These findings all indicate that the China's rural labor markets have been constantly improving in recent years, although there is still segmentation.Originality/valueIt is the first paper that uses a nationally representative survey data to address the development of rural labor market in the 21st century. With the help of a long-term panel data structure and by controlling the household-level fixed-effect, the authors obtained a deeper and more robust conclusion. Specifically, this article finds that whether it is for the off-farm transfer or the migration, the influence of labor age, gender, human capital and marital status is gradually weakening.
Biculturalism and cross-cultural competence: examination of an attribution complexity theory-based model
Authors: Lakshman C. , Lakshman Sangeetha , Gok Kubilay 
Published: October 2020

PurposeBased on attributional complexity (AC) theory, the authors empirically examine the impact of biculturalism on cross-cultural adjustment and the degree to which people make isomorphic attributions, critical for cross-cultural leadership effectiveness.Design/methodology/approachUsing questionnaire surveys, the authors first validate measures in a validation sample and then empirically test the model in a second sample, using structural equation modeling.FindingsThe authors’ findings reveal an empirical connection between biculturalism and AC on the one hand, while also showing support for the relationship between biculturalism and attributional knowledge. Findings also demonstrate that biculturalism is related to attributional accuracy in cross-cultural contexts via an attributional mechanism, as suggested by AC theory.Research limitations/implicationsFirst, AC theory emerges as one with excellent prospects for explaining intercultural work in multicultural settings. Biculturalism's links to AC and attributional knowledge are critical for extensions to cross-cultural leadership effectiveness, and international knowledge transfer, interesting and high potential research avenues for the discipline.Practical implicationsThe authors’ findings carry a host of managerial implications. AC training can provide all international assignees with the necessary foundational skills and learning abilities to successfully interact in the host country setting with local nationals. This study also suggests that managers on international assignments should focus their efforts on acquiring attributional knowledge because it can provide a solid boost to their understanding of the local culture.Originality/valueOne’s understanding of biculturals and their cross-cultural management competencies is very limited. The authors provide empirical support for the hypotheses, hitherto unexamined in extant literature.
Relevance of cryptomarkets, characteristics of purchasers and possibilities for survey research: experience from the European Web Survey on Drugs
Authors: Strizek Julian , Karden Alexandra , Matias João 
Published: October 2020

Purpose
The purpose of this paper is to assess the relevance of cryptomarkets, characteristics of purchasers and possibilities for survey research by approaching users directly on cryptomarkets.

Design/methodology/approach
Cross-country comparison of the results from the European Web Survey on Drugs (EWSD) and summarizing lessons learned during the data collection was carried out.

Findings
Purchasers of drugs on cryptomarkets are still a rather small segment of all drug purchasers, and most people who use cryptomarkets also use other sources of supply to buy drugs. The percentage of people using cryptomarkets is unevenly distributed across countries and substances. Purchasers on cryptomarkets in most countries are more likely to be men and more likely, on average, to use more substances. Other characteristics such as age or place of residence do not show a consistent pattern across countries. Recruitment of respondents on cryptomarkets calls for specific techniques and procedures. Specific attention should be paid to build trust and guarantee credibility and anonymity.

Research limitations/implications
Interpretation of the quantitative results is limited by nonprobabilistic sampling and different recruitment strategies in different countries.

Practical implications
Users of cryptomarkets show some specific characteristics, providing a challenge for research and prevention agencies to keep up with digital technology. Increasing knowledge about characteristics of users of cryptomarkets may help to create adequate responses for harm reduction measures in different supply settings. However, collecting self-reported data from users on cryptomarkets is limited owing to significant privacy concerns and requires specific skills and strategies.

Originality/value
The EWSD provides a rare opportunity for detailed analyses of consumption patterns and characteristics of active drug users across several European countries. Furthermore, experiences of a new recruitment strategy are discussed.
Transfer relations: useful basis for computer-aided engineering of circular arch structures
Authors: Zhang Jiao-Long , Liu Xian , Yuan Yong , Mang Herbert A. , Pichler Bernhard L.A. 
Published: October 2020

Purpose
Transfer relations represent analytical solutions of the linear theory of circular arches, relating each one of the kinematic and static variables at an arbitrary cross-section to the kinematic and static variables at the initial cross-section. The purpose of this paper is to demonstrate the significance of the transfer relations for structural analysis by means of three examples taken from civil engineering.

Design/methodology/approach
The first example refers to an arch bridge, the second one to the vault of a metro station and the third one to a real-scale test of a segmental tunnel ring.

Findings
The main conclusions drawn from these three examples are as follows: increasing the number of hangers/columns of the investigated arch bridge entails a reduction of the maximum bending moment of the arch, allowing it to approach, as much as possible, the desired thrust-line behavior; compared to the conventional in situ cast method, a combined precast and in situ cast method results in a decrease of the maximum bending moment of an element of the vault of the studied underground station by 46%; and the local behavior of the joints governs both the structural convergences and the bearing capacity of the tested segmental tunnel ring.

Originality/value
The three examples underline that the transfer relations significantly facilitate computer-aided engineering of circular arch structures, including arch bridges, vaults of metro stations and segmental tunnel rings.
New jerseys: exploring the influence of NBA team jersey sponsorships on teams and sponsors
Authors: Shoffner Sara , Slavich Mark , Koo Gi-Yong 
Published: October 2020

PurposeIn 2017, the National Basketball Association (NBA) became the first major professional North American sport league to adopt jersey sponsorship. While professional leagues across the globe have allowed this practice for decades, the NBA's decision marked what may be the start of a new trend in North American sport sponsorship. With this in mind, the current study sought to assess the impact of fans' perceptions of these sponsorships on sponsor- and team-related outcomes.Design/methodology/approachAn online sample of 301 NBA fans across the United States was conducted through Amazon's MTurk.FindingsResults exhibited the importance of sponsorship authenticity, which maintained the strongest influence on sponsor- and team-related outcomes. Brand–team fit, sponsorship familiarity and sponsorship identification were also assessed, with mixed results for each factor.Originality/valueResults of these findings related to sponsorship authenticity and consequent practical and theoretical implications are discussed, and areas for future research are provided.