bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of a Technology-based System to Motivate Aged Persons in Performing Physical Activity: A Feasibility Study
Authors: Knippenberg Els , Timmermans Annick , Palmaers Steven , Spooren Annemie 
Published: September 2020

Abstract
BackgroundMaintaining or initiating regular physical activity (PA) is important for successful aging. Technology-based systems may support and stimulate aged persons to initiate and persevere in performing PA. The aim of the current study was to assess to which extent a customised Kinect system is 1) a credible tool to increase PA in aged persons, 2) motivating to perform PA by aged persons, and 3) easy to be used in aged persons. MethodsA mixed-method cross-sectional feasibility study was performed in 5 aged care facilities in Flanders, Belgium. Aged participants were asked to perform a 20-30 minute test with the intelligent Activity-based Client-centred Training (i-ACT) system. After the test, the ‘Credibility and Expectancy Questionnaire’ (CEQ), the ‘Intrinsic Motivation Inventory’(IMI), the System Usability Scale (SUS), and semi-structured interviews were conducted in the aged participants. Feedback was gathered using the thinking aloud method in both aged participants and supervisors. ResultsA total of 48 aged persons (20 males and 28 females, mean age=81.19 (SD=8.10)), were included. The scores pertaining to system credibility and expectancy, system usability, and motivation towards use were moderate to good. Participants reported that they liked using the i-ACT system, but that the context could be more attractive by adding more visualisations. Twelve supervisors gave feedback regarding the i-ACT systems itself, the use and motivational aspects. ConclusionsThis study indicates that i-ACT is a useful and motivational system to engage aged persons to perform PA and therefore supports successful aging. Furthermore, i-ACT is a low-cost, easy to use and controller/wire-free system, which makes i-ACT a suitable tool for use in the home environment. Further development and future research is necessary to adapt the i-ACT system towards the wishes and needs of the aged population at home and in care facilities. Future research should include the effect of using a client-centred task-oriented approach in combination with technology-supported systems to engage aged persons to perform PA. Trial registrationClinicalTrial.gov ID NCT04489563, 23 July 2020 - Retrospectively registered, https://register.clinicaltrials.gov/prs/app/action/SelectProtocol?sid=S000A39C&selectaction=Edit&uid=U0003E6F&ts=37&cx=2jhfvs
Psychosocial factors associated with postpartum psychological distress during the Covid-19 pandemic: a cross-sectional study.
Authors: Ostacoli Luca , Cosma Stefano , Bevilacqua Federica , Berchialla Paola , Bovetti Marialuisa , Carosso Andrea Roberto , Malandrone Francesca , Carletto Sara , Benedetto Chiara 
Published: September 2020

Abstract
Background Trauma, natural and man-made catastrophic events can be predictors of postpartum psychological distress. In a public health response due to coronavirus disease 2019 outbreak, the Italian government imposed a lockdown from March 9 to May 3. This extraordinary situation may have been challenging for maternal psychological health. The aim of this study was to investigate the prevalence of depressive and post-traumatic stress symptoms in women giving birth during the Covid-19 pandemic and its associations with quarantine measures, obstetrical factors, and relational attachment style. Methods Women who gave birth in a high-volume obstetric/gynaecological medical centre located in an epidemic area during the Covid-19 pandemic (March 8 to June 15) were asked to complete an online survey about their childbirth experience and the perceived effect of the pandemic. The Edinburgh Postnatal Depression Scale (EPDS), the Impact of Event Scale-Revised (IES-R), and the Relationship Questionnaire (RQ) were administered to assess levels of postpartum depressive and post-traumatic stress symptoms (PTSS) and relational style of attachment, respectively. Multivariate analysis was applied to identify associations between quarantine measures, childbirth experience, attachment style, and EPDS and IES-R scores.Results The survey was completed by 163 women (response rate 60.8%). The prevalence of depressive symptoms was 44.2% (EPDS cut-off score ≥11) and the PTSS rate was 42.9% (IES-R cut-off score ≥24). Dismissive and fearful avoidant attachment styles were significantly associated with the risk of depression and PTSS, respectively. Perceived pain during delivery was a risk factor for postpartum depression. Perceived support provided by healthcare staff was a protective factor against depression and PTSS. Another protective factor against PTSS was quiet on the ward due to the absence of hospital visitors. Conclusion This study reports a high prevalence of postpartum depressive and PTSS in women who delivered during the Covid-19 pandemic. Postnatal psychological distress seemed to be associated more with the prenatal experience and other individual factors than with the pandemic hospital restrictions. Early detection during pregnancy of an insecure attachment style is fundamental to provide targeted preventive and therapeutic psychological interventions.
The Role of Social Support in the Association Between Dependency and the Important Risk Factors Among Elderly: A Cross-sectional Study Based on Baseline Data From Aehrr Project
Authors: Cui PinYu , Chen Yuan , Li YaXing , WaiLi Nuremaguli , Li Ying 
Published: September 2020

Abstract
BackgroundDependence is a common mental disorder among the elderly increases with increasing age，and can lead to serious health problems. This study aimed to assess the association between social resources and dependency, and explored the effect of social support on modification of important risk factors for dependency among elderly.Method The present study was a population-based cross-sectional study. A total of 950 participants aged ≥ 60 years were selected using a complex multistage sampling design from 22 locations in China. All data were collected using questionnaires by face-to-face interviews. The dependency was assessed using the standardized Minnesota Multiphasic Personality Inventory-II, and the social resource was assessed using the questionnaires of Older American Resources and Services (OARS) in Chinese version. The logistic regression analysis was used to evaluate the association between dependency and social resources. The analysis of covariance was performed to evaluate the effect of social support on modification of important risk factors for dependency.ResultsA total of 913 participants were included in the analysis, and 10.3% of males and 14.4% of females were identified as dependency. The results of logistic regression analysis showed that a significant negative association between the levels of social resource and dependency. The odds ratio was 0.78 (95% CI, 0.73–0.84) after adjusting for important confounding factors. The results of analysis of covariance showed the differences in the mean dependency score between different chronic disease status and social support level.ConclusionThe levels of social resources were negatively associated with dependency. Our results suggest that the social support had both a direct effect on dependency and an indirect effect through modifying chronic disease status and individual income.
Establishment and Evaluation of an Indirect ELISA for Detection of Antibodies to Goat Klebsiella Pneumonia
Authors: Chen Ruichang , Shang Hongqi , Niu Xiangyun , Huang Jin , Wang Huan , Miao Yongqiang , Sha Zhou , Zhu Ruiliang , Peng Duo , Hu Liping 
Published: September 2020

Abstract
Background: Klebsiella pneumonia, a Gram-negative bacterium belonging to the genus Enterobacter, causes many human and livestock diseases. Notably, infected goats may develop pneumonia, septicemia, which can lead to occasional death, resulting in great economic losses in goat-farming industry. However, there are no systematic methods for detection of Klebsiella pneumoniae in goats currently. Results: In this study, we developed a Klebsiella pneumoniae goat polyclonal antibody and established an indirect ELISA method to detect the Klebsiella pneumoniae. After screening and optimizing the conditions for detection, we determined the optimal working dilutions of the coated-bacterial antigen, the polyclonal antibody, and the enzyme-labeled secondary antibody that were 1:800 (2.99x107 CFU/ml), 1:6400, and 1:5000, respectively. The optimal condition of coating and blocking were both 4 °C for 12 h. The optimal dilution buffers of bacterial antigen, the antibodies, and the blocking buffer were 0.05 mol/L carbonate buffer, 1% BSA phosphate buffer, and 1.5% BSA carbonate buffer, respectively. The cut-off value was determined to be 0.28, and the analytical sensitivity was 1:800. Furthermore, there was no cross-reaction between the coated antigen and goat serum positive for other common bacteria, indicating that indirect ELISA could detect Klebsiella pneumoniae specifically in most cases. The average coefficients of variation of intra-assay and inter-assay were 4.37% and 5.17% indicating favorable reproducibility of indirect ELISA. In the detection of clinical veterinary samples, the positive rate of indirect ELISA was 6.74%, higher than that of conventional agglutination assays. Conclusions: Taken together, our results suggested that the method that we established has promising applications in production, laying the foundation for epidemiology, serological investigation and clinical veterinary diagnosis of Klebsiella pneumoniae in goats.
Genetic Diversity of a Natural Population of Akebia Trifoliata (Thunb.) Koidz and Extraction of a Core Collection Using Simple Sequence Repeat Markers
Authors: Zhong Yicheng , Wang Yue , Sun Zhimin , Niu Juan , Shi Yaliang , Huang Kunyong , Chen Jing , Luan Mingbao , Chen Jianhua 
Published: September 2020

Abstract
Background: Developing a core collection can deepen our understanding of the genetic diversity of germplasm resources and lay the foundation for their rational utilization. Akebia trifoliata (Thunb.) Koidz is an important oil crop, particularly in China, but there is no relevant research report about the core collection of A.trifoliate.Results: In this study, 28 simple sequence repeat (SSR) markers were used to assess the genetic diversity and genetic structure of a natural population of A. trifoliata, including 955 germplasms, and to extract a core collection. The genetic diversity of the natural population was moderately polymorphic. The average number of alleles (Na), observed heterozygosity (HO), expected heterozygosity (HE), Shannon’s information index (I*), and polymorphic information content (PIC) were 3.71, 0.24, 0.46, 0.81, and 0.41, respectively. Two sub-populations were identified, indicating a weak genetic structure. The core collection was composed of 164 individuals (17.2% of 955 total germplasms in the population), and diversity parameters differed significantly from those of a random core germplasm collection. Conclusions: The genetic diversity of the natural population of A.trifoliata was moderately polymorphic, and the genetic structure was weak. Moreover 164 individuals could represent the genetic diversity of the 995 individals. These results have implications for germplasm management and genomics studies in A. trifoliata as well as for the establishment of core collections of other perennial liana species.
Factors Associated with Acute and Persistent Psychological Disturbances During the COVID-19 Pandemic
Authors: Płomecka Martyna Beata , Gobbi Susanna , Neckels Rachael , Radziński Piotr , Skórko Beata , Lazzeri Samuel , Almazidou Kristina , Dedić Alisa , Bakalović Asja , Hrustić Lejla , Ashraf Zainab , haghi Sarvin Es , Rodríguez-Pino Luis , Waller Verena , Jabeen Hafsa , Alp A. Beyza , Behnam Mehdi A , Shibli Dana , Barańczuk Zofia , Haq Zeeshan , Qureshi Salah U , Strutt Adriana M. , Jawaid Ali 
Published: September 2020

Abstract
Background: The emergence of Corona virus disease (COVID-19) and wide-spread counter-measures, such as quarantine and social distancing, can have a significant impact on mental health of individuals. Methods: This online study anonymously screened 13,332 individuals worldwide for acute psychological symptoms related to Corona virus disease 2019 (COVID-19) pandemic from March 29th to April 14th, 2020. A total of n=12,817 responses were considered valid. n=1077 participants from Europe were screened a second time during May 15th to May 30th to longitudinally ascertain the persistence of psychological effects. Results: Female gender, pre-existing psychiatric condition, and prior exposure to trauma were identified as notable factors associated with increased acute psychological symptoms during COVID-19. The same factors, in addition to, being related to someone who demised due to COVID-19 and using social media more than usual predicted persistence of psychological disturbances. Optimism, ability to share concerns with family and friends like usual, positive prediction about COVID-19, and daily exercise predicted fewer acute and persistent psychological symptoms. Conclusions: Females, psychiatric patients, and individuals with previous exposure to trauma are at increased risk of being persistently psychologically affected by COVID-19.
A Reverse Transcription Loop-mediated Isothermal Amplification for Broad Coverage Detection of Asian and African Zika Virus Lineages
Authors: Teoh Boon-Teong , Chin Kim-Ling , Samsudin Nur-Izyan , Loong Shih-Keng , Sam Sing-Sin , Tan Kim-Kee , Khor Chee-Sieng , Abd-Jamil Juraina , Zainal Nurhafiza , Wilder-Smith Annelies , Zandi Keivan , AbuBakar Sazaly 
Published: September 2020

Abstract
Background: Early detection of Zika virus (ZIKV) infection during the viremia and viruria facilitates proper patient management and mosquito control measurement to prevent disease spread. Therefore, a cost-effective nucleic acid detection method for the diagnosis of ZIKV infection, especially in resource-deficient settings, is highly required.Methods: In the present study, a single-tube reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of both the Asian and African-lineage ZIKV. The detection limit, strain coverage and cross-reactivity of the ZIKV RT-LAMP assay was evaluated. The sensitivity and specificity of the RT-LAMP were also evaluated using a total of 24 simulated clinical samples. The ZIKV quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay was used as the reference assay.Results: The detection limit of the RT-LAMP assay was 3.73 ZIKV RNA copies (probit analysis, P ≤ 0.05). The RT-LAMP assay detected the ZIKV genomes of both the Asian and African lineages without cross-reacting with other arthropod-borne viruses. The sensitivity and specificity of the RT-LAMP assay were 90% (95% CI = 59.6 – 98.2) and 100% (95% CI = 78.5 – 100.0), respectively. The RT-LAMP assay detected ZIKV genome in 9 of 24 (37.5%) of the simulated clinical samples compared to 10 of 24 (41.7%) by qRT-PCR assay with a high level of concordance ( = 0.913, P < 0.001).Conclusion: The RT-LAMP assay is applicable for the broad coverage detection of both the Asian and African ZIKV strains in resource-deficient settings.
Functional Recovery in Hip Fracture Patients: a Real-practice Pilot Study Comparing Different Rehabilitation Settings
Authors: Freitas Margarida Mota , Curci Claudio , Ascenso Diana , Silveira Alda , Sire Alessandro de 
Published: September 2020

Abstract
Background: Hip fractures are a major source of disability in the elderly and rehabilitation is mandatory to recover pre-fracture functioning. To date, there is a big heterogeneity not only in terms of frequency and timing but also of choosing the most appropriate setting for hip fracture rehabilitation. In this real-practice pilot study we aimed at evaluating the long-term effects of rehabilitative intervention on disability status and functional outcomes in post-hip fracture elderly people.Methods: We included patients aged 65 years or older who had undergone surgical stabilization of a hip fracture. Exclusion criteria: pathological fractures or whose comorbidities could have an impact on motor, cognitive or sensory function. The sample was divided into 3 groups according to their destination after hospital discharge: Group 1, outpatient rehabilitation; Group 2, inpatient rehabilitation; Group 3, home-based rehabilitation. All three groups underwent a specific rehabilitation protocol for 3 times/week for 2 months. At the baseline (T0), at 3 months (T1), and at 6 months after fracture (T2), we assessed as outcome measures: Barthel Index (BI), Functional Ambulation Categories, passive and active range of motion of hip flexion and abduction, Medical Research Council scale (MRC) to assess muscle strength in hip flexion, hip abduction, and knee extension.Results: All three groups showed an average statistically significant improvement (p<0.05) in all the outcome measures compared to baseline, except for MRC of knee extension in group 3 at T1. Considering the between-group analysis, BI was significantly higher in Group 1 than in Group 2 at T2 (p=0.018).Conclusions: Taken together, our findings show that rehabilitation could lead to a significant improvement in functional recovery, independently from the settings, albeit outpatient rehabilitation seems to be the best option for hip fracture patients.
High number of RNA copies in asymptomatic individuals infected with SARS-CoV-2 in an area of the Colombian Caribbean
Authors: Mattar Salim , Martinez-Bravo Caty , Rivero Ricardo , Contreras Hector , Faccini_Martinez Alvaro , Guzman Camilo , Galeano Ketty , Alvis Nelson , Contreras Veronica , Arrieta German , Gonzalez Marco , Miranda Jorge , Ospina Martha Lucia , Camargo-Assis Francisco , Mercado-Reyes Marcela , Garay Evelyn , Garcia-Perez Alejandra , Lopez Yesica , Tique Vaneza 
Published: September 2020

Abstract
Background. Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) is an emerging viral pandemic disease. In the last six months, SARS-CoV-2 has caused millions of reported cases and hundreds of thousands of deaths. As other world regions, nowadays, South America has not contained the pandemic's advance since it lacks the hospital and economic capacities. Public health implications of transmission, while the asymptomatic infection is a critical concern at the current pandemic.Objective: Describe the socio-demographic, clinical, and viral kinetics features of a cohort of SARS-CoV-2 infected individuals from the Colombian Caribbean. Methods: Six hundred eighty-six clinical samples of suspected SARS-CoV-2 infection cases and contacts individuals from several hospital centers in the department of Córdoba, Colombia, were received at our laboratory between April 9th and May 16th, 2020. RNA was extracted using lysis buffers and spin columns. The samples were tested for SARS-CoV-2 by RT-qPCR (Reverse transcription real-time polymerase chain reaction) using commercially available multiplex real-time PCR assay for simultaneous detection of 3 target genes of SARS-CoV-2 (AllplexTM, 2019-nCoV assay, Korea). Viral copies quantification was done using a standard curve constructed from seriated dilutions of a SARS-CoV-2 positive control. Statics descriptive methods were used. Results: Thirty-five nasopharyngeal samples were positive for SARS-CoV-2 infection; the average age was 43 (range, 1-95 years). Seventeen of 35 (49%) of the patients showed symptoms. Most of them had a cough, fever, and odynophagia; three of the patients reported having arthralgia. Only two patients required hospitalization. None of the patients had known co-morbidities. RT-qPCR results show that two of the symptomatic patients had significantly higher RNA copies than the rest. Eighteen of 35 (51%) individuals were asymptomatic, and the average age was 30 (range, 6-61 years). Four asymptomatic individuals showed a higher copy than some symptomatic patients; nonetheless, the average of RNA copies 8.26 x10+10 was lower than the symptomatic.Conclusions: the population studied was young, with an average of 43 years in symptomatic and 30 years of asymptomatic; this is important because of the high impact in the economy. Because a large proportion of infections may result from transmission from asymptomatic or pre-symptomatic persons, the usefulness of public health interventions in Colombian provinces should be based on molecular screening in a vast conglomerate population and quantify the viral load.
Targeting RNF8 Effectively Reverse Cisplatin and Doxorubicin Resistance in Endometrial Cancer
Authors: Yang Ben , Ke Wang , Wan Yingchun , Li Tao 
Published: September 2020

Abstract
Background
Endometrial cancer (EC) is one of the most frequent gynecological malignancy worldwide. However, resistance to chemotherapy remains one of the major difficulties in the treatment of EC. Thus, there is an urgent requirement to understand mechanisms of chemoresistance and identify novel regimens for patients with EC.
Methods
Cisplatin and doxorubicin resistant cell lines were acquired by continuous exposing parental EC cells to cisplatin or doxorubicin for 3 months. Cell viability was determined by using MTT assay. Protein Expression levels of protein were examined by western blotting assay. mRNA levels were measured by quantitative polymerase chain reaction (qPCR) assay. Ring finger protein 8 (RNF8) knockout cell lines were generated by clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 gene editing assay. Nonhomologous end joining (NHEJ) efficiency were quantified by plasmid based NHEJ assay. DNA double strand breaks (DSB) were generated using laser micro-irradiation. Protein recruitment to DSB was analyzed by immunofluorescent assay. Tumor growth was examined by AN3CA xenograft mice model.
Results
We found that protein and mRNA expression levels of RNF8 were significantly increased in both cisplatin and doxorubicin resistant EC cells. Cell survival assay showed that RNF deficiency significantly enhanced the sensitivity of resistant EC cells to cisplatin and doxorubicin (P < 0.01). In addition, chemoresistant EC cells exhibited increased NHEJ efficiency. Knockout of RNF8 in chemoresistant EC cells significantly reduced NHEJ efficiency and prolonged Ku80 retention on DSB. Moreover, cisplatin resistant AN3CA xenograft showed that RNF8 deficiency overcame cisplatin resistance.
Conclusions
Our in vitro and in vivo assays provide evidence for RNF8, which is a NHEJ factor, serving as a promising, novel target in EC chemotherapy.