bluuebunny/crossref_metadata_2025_split huggingface dataset:
Baseline factors identified for prediction of good responders in patients with end-stage diffuse coronary artery disease undergoing intracoronary CD34+ cell therapy
Authors: Sung Pei-Hsun , Chiang Hsin-Ju , Li Yi-Chen , Chiang John Y , Chu Chi-Hsiang , Shao Pei-Lin , Lee Fan-Yen , Lee Mel S. , Yip Hon-Kan 
Published: July 2020

Abstract
Background: Treating patients with end-stage diffuse coronary artery disease (EnD-CAD) unsuitable for coronary intervention remains a clinical challenge. They usually express refractory angina and have high risk for mortality. Although growing data have indicated cell therapy is an alternative solution to medical or invasive therapy, there are still lacking useful markers to predict whether heart function will improve in the EnD-CAD patients who underwent circulatory-derived CD34+ cell therapy. By utilizing the baseline variables and results from our previous phase I/II clinical trials, the aim of this study tried to elucidate the variables predictive of the “good response” to CD34+ cell therapy.Methods: This retrospective study included 38 patients in the phase I clinical trial (2011-2014), and 30 patients in the phase II clinical trial (2013-2017). These patients were categorized into “good responders” and “non-responders” according to their 1-year improvement of LVEF ≥7.0% or <7.0% after intracoronary CD34+ cell therapy. Univariate and multivariate logistic regression models were performed to identify potential independent predictors of good responder to cell therapy, followed by Hosmer–Lemeshow (H-L) test for goodness of fit and prediction power.Results: Among baseline data, multivariate analysis demonstrated that history of former smoker was independently predictive of good responders (p=0.006). On the other hand, male gender, the baseline Canadian Cardiovascular Society angina score ≥3 and grades of LV diastolic dysfunction ≥2 were significantly negative predictors of good responders (all p<0.01). After administration of subcutaneous granulocyte-colony stimulating factor (G-CSF), a higher post-G-CSF neutrophil count in addition to the above four baseline variables also played crucial roles in early prediction of good response to CD34+ cell therapy for EnD-CAD (all p<0.03). The H-L test displayed a good prediction power with sensitivity 83.3%, specificity 85.3% and accuracy 84.4%. Conclusions: Using the results of our phase I/II clinical trials, previous smoking habit, female sex, lower grades of angina score and diastolic dysfunction were identified to be independently predictive of “good response” to CD34+ cell therapy in the patients with EnD-CAD.Trial registration: This is a retrospective analysis based on the phase I (ISRCTN72853206) and II (ISRCTN26002902) clinical trials
A New Lc-Ms/Ms Method for Multiple Residues/Contaminants In Bovine Meat
Authors: Hajrulai-Musliu Zehra , Uzunov Risto , Jovanov Stefan , Jankuloski Dean , Stojkovski Velimir , Pendovski Lazo , Sasanya James Jacob 
Published: July 2020

Abstract
A multi-class and multi-residue/contaminant method for the determination of veterinary drug and pesticide residues and mycotoxins in bovine meat has been developed and validated. The veterinary drug residues/contaminants included antimicrobials, anabolic hormones, lactones, β-agonists, mycotoxins, and pesticides. Isotopic labeled internal standards were included to compensate residual matrix effects. The calibrators used in the method demonstrated linearity with the coefficient of correlation (R2) > 0.98. The decision limit (CCα) values were in the range from 0.067 to 2103.84 μg/kg, while the range for detection capability (CCβ) was from 0.083 to 2482.13 μg/kg. The limit of detection (LOD) and limit of quantification (LOQ) were in the range from 0.059 to 291.36 μg/kg, and 0.081 to 328.13 μg/kg, respectively. The recovery of analytes ranged from 61.28 % to 116.20 %. The intra-day coefficient of variation (CV) was from 0.97 to 25.93 % and the inter-day CV was 2.30-34.04 %. The method has been used for the determination of 49 residues/contaminants in bovine meat.
miR-18a-5p promotes NPC cell proliferation, invasion, migration, and EMT by targeting SMAD2
Authors: Li Pengcheng , Xing Junhui , Jiang Jianwu , Tian Xinyu , Liu Xuemeng , Yao Rui , Wu Leiming , Liang Cui , Zhang Yanzhou , Liu Junqi 
Published: July 2020

Abstract
Background

Nasopharyngeal carcinoma (NPC) is the most common malignant tumor in the head and neck that is characterized by high local malignant invasion and distant metastasis. miR-18a-5p reportedly plays an important role in tumorigenesis and development. However, little is known about the mechanism underlying miR-18a-5p’s role in NPC.

Methods

Quantitative real-time PCR was used to detect the expression of miR-18a-5p in NPC tissues and cell lines. MTT assay and plate clone formation assay were used to detect the effect of miR-18a-5p on NPC cell proliferation. Wound healing assays and Transwell assays were used to detect the effect of miR-18a-5p on NPC cell invasion and migration. The expressions of epithelial mesenchymal transition (EMT)-related proteins N-cadherin, Vimentin, and E-cadherin were detected by Western blot. Bioinformatics and dual-luciferase reporter assay were used to detect the targeting interaction between miR-18a-5p and SMAD2. Xenotransplantation and metastasis model were used to detect the effect of miR-18a-5p on NPC growth and metastasis in vivo.

Results

miR-18a-5p was highly expressed in NPC tissues and cell lines. Overexpression of miR-18a-5p promoted NPC cell proliferation, invasion, migration, and EMT process, whereas inhibition of miR-18a-5p expression led to the opposite results. Results of dual-luciferase reporter assay showed that SMAD2 was the target gene of miR-18a-5p, and SMAD2 could reverse the effect of miR-18a-5p on NPC cell line. Xenotransplantation and metastasis model experiments in nude mice showed that miR-18a-5p promotes NPC growth and metastasis in vivo.

Conclusions

Targeting SMAD2 downregulated miR-18a-5p expression, thereby promoting NPC cell proliferation, invasion, migration, and EMT.
Characteristics of patients who access zero, one or multiple General Practices and reasons for their choices: a study in regional Australia
Authors: Glenister Kristen Maree , Guymer John , Bourke Lisa , Simmons David 
Published: July 2020

Abstract
Background:
Most Australians visit a General Practitioner annually and are free to choose their General Practitioner and/or practice on each occasion. A proportion of people visit multiple general practices, which can reduce continuity of care, a core value of general practice. Utilisation of multiple general practices is associated with metropolitan residence and younger age. However, it is unclear which factors are associated with utilisation of multiple general practices in rural areas, where there are often General Practitioner workforce shortages and higher proportions of patients who may benefit from continuity of care such as older people or those with chronic disease. The aim of this study was to compare the characteristics of people in a rural Australian area who accessed multiple general practices in the previous year.
Methods:
A cross-sectional survey assessed self-reported utilisation and perspective of general practice services, uses of multiple practices, associated reasons, lifestyle advice and screening services received in four regional Victorian towns. Households were randomly selected and residents aged 16 + were eligible to participate.
Results:
Compared with utilisation of a single general practice, multiple general practice attendance in the past year (14.3%) was associated with younger age (adjusted odds ratio (aOR 95% confidence interval) 0.98 per year (0.97–0.99), residence in the regional centre aOR 2.90(2.22–3.78), emergency department (ED) attendance in the last 12 months aOR 1.65(1.22–2.21) and no out of pocket costs aOR 1.36(1.04–1.79)). Reasons for multiple general practice attendance included availability of appointments, cost and access to specific services. Compared with multiple general practice attendance, those attending single practices reported more screening tests but similar frequency of lifestyle advice. People who accessed multiple practices were less likely to report very high satisfaction (51.7% vs 62.9% p < 0.001) or excellent degree of confidence in their doctor (42.0% vs 49.8% p = 0.006) than single practice attendees.
Conclusions:
Those attending single practices report higher satisfaction and confidence in their GP and were less likely to attend ED. Further studies are required to test whether increasing availability of appointments and reducing out-of-pocket expenses would increase single practice attendance and/or decrease healthcare costs overall.
Ticagrelor Monotherapy in Patients with Concomitant Diabetes Mellitus and Chronic Kidney Disease: A post Hoc Analysis of The GLOBAL LEADERS Trial
Authors: Gao Chao , Tomaniak Mariusz , Takahashi Kuniaki , Kawashima Hideyuki , Wang Rutao , Hara Hironori , Ono Masafumi , Montalescot Gilles , Garg Scot , Haude Michael , Slagboom Ton , Vranckx Pascal , Valgimigli Marco , Windecker Stephan , Geuns Robert-Jan van , Hamm Christian , Steg Philippe Gabriel , Onuma Yoshinobu , Angiolillo Dominick , Serruys Patrick W. 
Published: July 2020

Abstract
Background: Patients with both diabetes mellitus (DM) and chronic kidney disease (CKD) are a subpopulation characterized by ultrahigh ischemic and bleeding risk after the percutaneous coronary intervention (PCI). There are limited data on the impact of ticagrelor monotherapy among these patients.Methods: In this post hoc analysis of the GLOBAL-LEADERS trial, the treatment effects of the experimental (one-month dual-antiplatelet therapy [DAPT] followed by 23-month ticagrelor monotherapy) versus the reference regimen (12-month DAPT followed by 12-month aspirin alone) were analyzed according to DM/CKD status. The primary endpoint was a composite endpoint of all-cause death or new Q-wave myocardial infarction at two-years. The patient-oriented composite endpoint (POCE) was defined as the composite of all-cause death, any stroke, site-reported MI and any revascularization, whereas net adverse clinical events (NACE) combined POCE with BARC type 3 or 5 bleeding events.Results: At two years, the DM+/CKD+ patients had significantly higher incidences of the primary endpoint (9.5% versus 3.1%, adjusted HR 2.16; 95%CI [1.66-2.80], p<0.001), BARC type 3 or 5 bleeding events, stroke, site-reported myocardial infraction, all revascularization, POCE, and NACE, compared with the DM-/CKD- patients. Among the DM+/CKD+ patients, after adjustment, there were no significant differences in the primary endpoints between the experimental and reference regimen. The experimental regimen was associated with lower rates of POCE (20.6% versus 25.9%, HR 0.74; 95%CI [0.55-0.99], p=0.043, ptrend=0.155) and NACE (22.7% versus 28.3%, HR 0.75; 95%CI [0.56-0.99], p=0.044, ptrend=0.310), mainly driven by a lower rate of all revascularization, as compared with the reference regimen. The landmark analysis showed that while the experimental and reference regimen had similar rates of all the clinical endpoints during the first year, the experimental regimen was associated with significantly lower rates of POCE (5.8% versus 11.0%, HR 0.49; 95%CI [0.29-0.82], p=0.007, ptrend=0.040) and NACE (5.8% versus 11.2%, HR 0.48; 95%CI [0.29-0.82], p=0.007, ptrend=0.013) in the second year.Conclusion: Among patients with both DM and CKD, ticagrelor monotherapy was not associated with lower rates of all-cause death or new Q-wave, or major bleeding complications; however, it was associated with lower rates of POCE and NACE. These findings should be interpreted as hypothesis-generating.Clinical Trial Registration: ClinicalTrials.gov (NCT01813435).
Epidemiology and Multidrug Resistance of Strongyle Nematodes in Ordos Merino Sheep
Authors: Eye Ledege , Wuen Jiya , He Xiuling , Buyin Buhe , Hai Ying , HASI Surong 
Published: July 2020

Abstract
Background: Regular whole-flock treatments and long-term repeated use of similar anthelmintics has led to the development of anthelmintic resistance and widespread epidemic of sheep nematodiasis. The current work was carried out to understand the prevalence of gastrointestinal nematodes in Ordos merino sheep and to evaluate the efficacy of currently used anthelmintics. Results: A total of 4014 fresh fecal samples were collected from sheep from March 2017 to April 2019 and fecal nematode eggs were qualitatively and quantitatively analyzed for understanding the prevalence of sheep nematodiasis. The anthelmintic efficacy of currently used drugs was evaluated in naturally infected sheep. The gastrointestinal nematode infection was serious in Ordos merino sheep. The infection rates of nematodes in three consecutive years were 84.3%, 36.9%, and 42.3%, respectively. The Hemonchus contortus and Nematodirus sp. were the dominantly infected species with the infection rates of 84.3% and 65.6% in 2017. Moreover, these species were acquired highly resistance to the Ivermectin, Doramectin, Albendazole and Levamisole. The percentages of FERC were 6.9%, 1.7%, 3.5% and 79.0%, respectively, following single administration. However, Nitroxynil and Closantel showed a strong anthelmintic efficacy on popular dominant species Haemonchus contortus and some other nematodes, while almost no effect on Nematodirus sp. Conclusions: The prevalence of gastrointestinal nematodes in Ordos merino sheep and their resistance to commonly used anthelmintics were comprehensively understood, and the high efficacy drugs on predominant species were screened out. The results will pay a good foundation for developing of reasonable drug use.
PCNA-associated Factor (KIAA0101/p15PAF) over expression and Gene Copy Number Alterations in Hepatocellular Carcinoma Tissues
Authors: Tantiwetrueangdet Anchalee , Panvichian Ravat , Sornmayura Pattana , Leelaudomlipi Surasak 
Published: July 2020

Abstract
Background
PCNA-associated factor (KIAA0101/p15PAF) is a cell-cycle regulated oncoprotein that regulates DNA synthesis, maintenance of DNA methylation, and DNA-damage bypass, through the interaction with the human sliding clamp PCNA. KIAA0101 is overexpressed in various cancers, including hepatocellular carcinoma (HCC). However, it remains unknown whether KIAA0101 gene amplification occurs and causally correlates with the KIAA0101 overexpression in HCC. This question is relevant to the development of the optimal test(s) for KIAA0101 and the strategies to target KIAA0101 in HCC.
Methods
In this study, we validated KIAA0101 mRNA expression levels by quantitative real-time PCR in 40 pairs of snap-frozen HCC and matched-non-cancerous tissues; we then evaluated KIAA0101 gene copy numbers by droplet digital PCR (ddPCR) in 36 pairs of the tissues. Besides, KIAA0101 protein expression was detected by immunohistochemistry (IHC) in 81 pairs of formalin-fixed paraffin-embedded (FFPE) tissues. We determined the correlation between KIAA0101 gene copy numbers and KIAA0101 RNA expression by Spearman correlation, and between KIAA0101 protein expression and other clinicopathological variables by Chi-square test.
Results
Our results confirmed KIAA0101 mRNA overexpression in HCC; KIAA0101 mRNA levels were significantly higher in HCC than in the matched-non-cancerous tissues (p < 0.0001). We found no amplification of KIAA0101 gene in HCC and no correlation between KIAA0101 gene copy numbers and KIAA0101 RNA expression. KIAA0101 protein was overexpressed in the majority of HCC tissues (77.8%) but was not be detectable in all of the matched-non-cancerous tissues. Significant correlations between the expression of KIAA0101 protein and p53 tumor suppressor protein, and Ki-67 proliferation marker protein were found (p = 0.002 and 0.017, respectively), but no correlations between the expression of KIAA0101 and age, tumor size, and expression of AFP and HBsAg.
Conclusions
KIAA0101 overexpression, at mRNA and protein levels, frequently occurs in HCC without concurrent KIAA0101 gene amplification. Significant correlations between the expression of KIAA0101 protein and p53 and Ki-67 proteins were observed in this study. Thus, detection of KIAA0101 mRNA/protein might be used, along with the detection of p53 and Ki-67 proteins, as potential biomarkers to select candidate patients for further studies of novel HCC treatment related to these targets.
Maternal Health Care Seeking Behavior of Nepalese Woman: A Perspective of Socio Ecological Model
Authors: Sharma Mohan Kumar , Khanal Shanti Prasad , Acharya Jib , Adhikari Ramesh , Bista Rabi , Adhikari Deep Bahadur , Kheral Suman 
Published: July 2020

Abstract
Background Nepal has a very high Maternal Mortality Ratio (MMR) in the South Asian region partly owing to the low utilization of maternal health services. One of the leading causes for maternal death in the rural areas of Nepal is lack of awareness about mental health. The prominent objective of this study is to explore the influencing factors utilization of maternal health care service among Nepalese women.Methods This is a qualitative study performed in Kathmandu district at Tribhuvan University Teaching Hospital, in Nepal. In-depth interviews (IDIs) were administrated with 18 women with recent delivery case (within seven days). Furthermore, five Key-informants interview (KIIs) with their husbands was conducted. The data was thematically analyzed using content analysis, where Social-Ecological Model (SEM) was applied as a theoretical framework to lead thematic content.Results Women's knowledge, perception, decision-making autonomy in interpersonal level, mother-in-law and husband's role in intrapersonal level, employment organization in institutional level, peer groups, and neighbors in community level, and safe-motherhood program in policy level were influencing factors to obey adequate Maternal Health Care Seeking Behavior (MHCSB). Also, negligence of women in MHC check-up, inadequate health facilities, health facilities without maternal requirement and non availability gynecologist were observed as a core barrier for utilization of MHCSB.Conclusions There were numerous causes for not utilizing MHCSB: inadequate health facilities, health facilities without maternal services, and unavailability of gynecologist especially in health facilities of rural areas in Nepal. More health facilities should be built especially in remote areas and adequately equipped with maternal health services, drugs, and specialist. Further, existing health facilities should be promoted with overall maternal health care requirement with its specialist. Free MHCS should cover the cost of items required for delivery in addition to ANC, PNC check-up and institutional delivery.
Routine Use of Immunosuppressants is Associated with Mortality in Hospitalised Patients with COVID-19
Authors: Myint Phyo K , Carter Ben , Barlow-Pay Fenella , Short Roxanna , Einarsson Alice G , Bruce Eilidh , McCarthy Kathryn , Verduri Alessia , Collins Jemima , Hesford James , Rickard Frances , Mitchell Emma , Halloway Mark , McGovern Aine , Vilches-Moraga Arturo , Braude Philip , Pearce Lyndsay , Stechman Michael , Price Angeline , Quinn Terence J , Clini Enrico , Moug Susan J , Hewitt Jonathan 
Published: July 2020

Abstract
Background: Whilst there is literature on impact of the SARS viruses in the severely immunosuppressed, and those who develop exaggerated immune response, less is known about the link between routine immunosuppressant use and outcome in COVID-19. Consequently, guidelines on their use vary depending on specific patient populations.Methods: The study population was drawn from the COPE Study (COVID-19 in Older People), a multicentre observational cohort study, carried out in UK and Italy. Data were collected between 27th February and 28th April 2020 by trained data collectors and included all unselected consecutive admissions with Covid-19. Load (name/number of medications) and dosage of immunosuppressant were collected along with other covariate data. The primary outcome was time-to-mortality from the date of admission (or) date of diagnosis, if diagnosis was five or more days after admission. Secondary outcomes were Day-14 mortality and time-to-discharge (length of stay). Data were analysed with mixed-effects, Cox proportional hazards and Logistic regression models using non-users of immunosuppressants as the reference group.Results: 1184 patients were eligible for inclusion. The median (IQR) age was 74(62-83), 676(57%) were male, and 299(25.3%) died in hospital (total person follow-up 15,540 days). Most patients exhibited at least one comorbidity, and 113(~10%) were on immunosuppressants. We found that any immunosuppressant use was associated with increased mortality: aHR 1.87,95%CI:1.30,2.69 (time to mortality) and aOR1.71,95%CI:1.01-2.88 (14-day mortality). There also appeared to be a dose-response relationship.Conclusion: Despite the possibility of indication bias, until further evidence emerges we recommend adhering to public health measures stringently, a low threshold to seek medical advice and close monitoring of worsening symptoms in those who take immunosuppressants routinely regardless of their indication.
Development and Validation of a Novel Nomogram to Predict Lymph Node Involvement in T1 Stage Lung Adenocarcinoma.
Authors: Wang Jianjun , Wei Li , Li Jiwei , Zhang Quan , Ma Zeheng 
Published: July 2020

Abstract
A total of 326 patients with T1 lung adenocarcinoma from March 2012 to April 2016 in our center were included. The relationship between LNI and different risk factors were accessed by univariate and multivariate logistic regression analyses. Four significant risk factors identified by multivariate logistic regression were tumor diameter (OR = 2.175, 95%CI:1.277–3.072, p = 0.0045), lymph node swelling exist preoperative (OR = 6.144, 95%CI:6.947–14.059, p = 0.003), platelet to lymphocyte ratio (OR = 3.149, 95%CI:1.546–6.673, p = 0.003), CEA (OR = 4.375, 95%CI: 2.613–7.537, p = 0.00694). A nomogram was constructed by combing risk factors and validated with an internal set. The C-index of this nomogram was 0.875, which was validated by bootstrap method. At last we concluded that the novel nomogram showed the potential value of LNI prediction for lung adenocarcinoma.
Abnormal Regional Homogeneity and Its Relationship with Symptom Severity in Cervical Dystonia: A Rest State fMRI Study
Authors: Wei Shubao , Lu Chunhui , Chen Xiuqiong , Yang Lu , Wei Jing , Jiang Wenyan , Liu Yang , Li Huihui , Qin Yuhong , Lei Yiwu , Qin Chao , Hu Caiyou , Luo Shuguang 
Published: July 2020

Abstract
Background: Although several brain networks play important roles in cervical dystonia (CD) patients, regional homogeneity (ReHo) changes in CD patients have not been clarified. We investigated to explore ReHo in CD patients at rest and analyzed its correlations with symptom severity as measured by Tsui scale.Methods: A total of 19 CD patients and 21 gender-, age-, and education-matched healthy controls underwent fMRI scans at rest state. Data were analyzed by ReHo method.Results: Patients showed increased ReHo in the right cerebellum crus I and decreased ReHo in the right superior medial prefrontal cortex (MPFC). Moreover, the right precentral gyrus, right insula, and bilateral middle cingulate gyrus also showed increased ReHo values. A significantly positive correlation was observed between ReHo value in the right cerebellum crus I and symptom severity (p < 0.05).Conclusions: Our investigation suggested abnormal ReHo existed in brain regions of default mode network (i.e. the cerebellum and MPFC), the motor network (the right precentral gyrus) and imbic system (the right insula and bilateral middle cingulate gyrus) and further highlighted the significance of these networks in the pathophysiology of CD.