bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced histone H3 acetylation of the PD-L1 promoter via the COP1/c-Jun/HDAC3 axis is required for PD-L1 expression in drug-resistant cancer cells
Authors: Wang Haifang , Fu Chen , Du Jun , Wang Hongsheng , He Rui , Yin Xiaofeng , Li Haixia , Li Xin , Wang Hongxia , Li Kui , Zheng Lei , Liu Zongcai , Qiu Yurong 
Published: February 2020

Abstract
Background
Drug resistance is a major obstacle to treating cancers because it desensitizes cancer cells to chemotherapy. Recently, attention has been focused on changes in the tumor immune landscape after the acquisition of drug resistance. Programmed death-ligand-1 (PD-L1) is an immune suppressor that inhibits T cell-based immunity. Evidence has shown that acquired chemoresistance is associated with increased PD-L1 expression in cancer cells. However, the underlying mechanism is still largely unknown.

Methods
PD-L1 expression in three drug-resistant A549/CDDP, MCF7/ADR and HepG2/ADR cell lines was detected by qRT-PCR, western blotting and flow cytometry, and a T cell proliferation assay was performed to test its functional significance. Then, the potential roles of JNK/c-Jun, histone H3 acetylation, histone deacetylase 3 (HDAC3) and the E3 ligase COP1 in the PD-L1 increase were explored through ChIP assays and gain- and loss-of-function gene studies. Furthermore, murine xenograft tumor models were used to verify the role of JNK/c-Jun and HDAC3 in PD-L1 expression in A549/CDDP cells in vivo. Finally, the correlations of PD-L1, c-Jun and HDAC3 expression in clinical cisplatin-sensitive and cisplatin-resistant non-small cell lung cancer (NSCLC) tissues were analyzed by immunohistochemistry and Pearson’s correlation coefficient.

Results
PD-L1 expression was significantly increased in A549/CDDP, MCF7/ADR and HepG2/ADR cells and was attributed mainly to enhanced JNK/c-Jun signaling activation. Mechanistically, decreased COP1 increased c-Jun accumulation, which subsequently inhibited HDAC3 expression and thereby enhanced histone H3 acetylation of the PD-L1 promoter. Furthermore, PD-L1 expression could be inhibited by JNK/c-Jun inhibition or HDAC3 overexpression in vivo, which could largely reverse inhibited CD3+ T cell proliferation in vitro. PD-L1 expression was significantly increased in the cisplatin-resistant clinical NSCLC samples and positively correlated with c-Jun expression but negatively correlated with HDAC3 expression.

Conclusions
Enhanced histone H3 acetylation of the PD-L1 promoter via the COP1/c-Jun/HDAC3 axis was crucial for the PD-L1 increase in drug-resistant cancer cells. Our study reveals a novel regulatory network for the PD-L1 increase in drug-resistant cancer cells and that combined PD-L1-targeting strategies could improve T cell-based immunity in drug-resistant cancers.
RETRACTED ARTICLE: MiR-140 targets RAP2A to enable the proliferation of insulin-treated ovarian granulosa cells
Authors: Xiong Zhengfang , Li Bing , Wang Wenjuan , Zeng Xianghui , Li Binye , Jian Shengyan , Wang Liyun 
Published: February 2020

Abstract
Background
We elucidated the role of specific MicroRNAs (miRNAs) in the development of polycystic ovary syndrome (PCOS) and explained the changes in the proliferation of granulosa cells. Excised ovarian cortex specimens were collected for miRNA profiling analysis (n = 20 PCOS females and 5 non-PCOS females). Insulin-treated ovarian granulosa cells isolated from mice were used for mechanical studies.

Results
High miR-140 expression was observed in PCOS samples and insulin-treated granulosa cells compared to that in non-PCOS and unstimulated cells, respectively. However, the Ras-related protein Rap-2a precursor (RAP2A) was downregulated in in PCOS. MTT assay and EdU staining showed that an miR-140 inhibitor attenuated viability in insulin-treated granulosa cells; cell viability increased with miR-140 overexpression. Reduced expression of miR-140 and the expression of the miR-140 mimic resulted in marked cell apoptosis, as evidenced by the results of PI flow cytometry and Annexin V-FITC; miR-140 overexpression results in downregulated RAP2A expression, and the miR-140 mimic directly bound to the RAP2A 3′-UTR, causing increase in RAP2A levels in insulin-treated granulosa cells; RNA-mediated silencing of RAP2A in insulin-treated granulosa cells restored cell proliferation and apoptosis to normal levels. Phosphorylated AKT was found to be negatively regulated through cross-talk between miR-140 and RAP2A.

Conclusions
In conclusion, PCOS ovarian cortex specimens and insulin-treated granulosa cells showed elevated expression of miR-140, which could lead to increased proliferation and reduced apoptosis of cells by targeting RAP2A. This study may pave the way for future research on the properties of granulosa cells in PCOS.
Nanopore sequencing-based genome assembly and evolutionary genomics of circum-basmati rice
Authors: Choi Jae Young , Lye Zoe N. , Groen Simon C. , Dai Xiaoguang , Rughani Priyesh , Zaaijer Sophie , Harrington Eoghan D. , Juul Sissel , Purugganan Michael D. 
Published: February 2020

Abstract
Background
The circum-basmati group of cultivated Asian rice (Oryza sativa) contains many iconic varieties and is widespread in the Indian subcontinent. Despite its economic and cultural importance, a high-quality reference genome is currently lacking, and the group’s evolutionary history is not fully resolved. To address these gaps, we use long-read nanopore sequencing and assemble the genomes of two circum-basmati rice varieties.

Results
We generate two high-quality, chromosome-level reference genomes that represent the 12 chromosomes of Oryza. The assemblies show a contig N50 of 6.32 Mb and 10.53 Mb for Basmati 334 and Dom Sufid, respectively. Using our highly contiguous assemblies, we characterize structural variations segregating across circum-basmati genomes. We discover repeat expansions not observed in japonica—the rice group most closely related to circum-basmati—as well as the presence and absence variants of over 20 Mb, one of which is a circum-basmati-specific deletion of a gene regulating awn length. We further detect strong evidence of admixture between the circum-basmati and circum-aus groups. This gene flow has its greatest effect on chromosome 10, causing both structural variation and single-nucleotide polymorphism to deviate from genome-wide history. Lastly, population genomic analysis of 78 circum-basmati varieties shows three major geographically structured genetic groups: Bhutan/Nepal, India/Bangladesh/Myanmar, and Iran/Pakistan.

Conclusion
The availability of high-quality reference genomes allows functional and evolutionary genomic analyses providing genome-wide evidence for gene flow between circum-aus and circum-basmati, describes the nature of circum-basmati structural variation, and reveals the presence/absence variation in this important and iconic rice variety group.
Image-guided breast biopsy and localisation: recommendations for information to women and referring physicians by the European Society of Breast Imaging
Authors:   for the European Society of Breast Imaging (EUSOBI), with language review by Europa Donna–The European Breast Cancer Coalition, Bick Ulrich , Trimboli Rubina M. , Athanasiou Alexandra , Balleyguier Corinne , Baltzer Pascal A. T. , Bernathova Maria , Borbély Krisztina , Brkljacic Boris , Carbonaro Luca A. , Clauser Paola , Cassano Enrico , Colin Catherine , Esen Gul , Evans Andrew , Fallenberg Eva M. , Fuchsjaeger Michael H. , Gilbert Fiona J. , Helbich Thomas H. , Heywang-Köbrunner Sylvia H. , Herranz Michel , Kinkel Karen , Kilburn-Toppin Fleur , Kuhl Christiane K. , Lesaru Mihai , Lobbes Marc B. I. , Mann Ritse M. , Martincich Laura , Panizza Pietro , Pediconi Federica , Pijnappel Ruud M. , Pinker Katja , Schiaffino Simone , Sella Tamar , Thomassin-Naggara Isabelle , Tardivon Anne , Ongeval Chantal Van , Wallis Matthew G. , Zackrisson Sophia , Forrai Gabor , Herrero Julia Camps , Sardanelli Francesco 
Published: February 2020

AbstractWe summarise here the information to be provided to women and referring physicians about percutaneous breast biopsy and lesion localisation under imaging guidance. After explaining why a preoperative diagnosis with a percutaneous biopsy is preferred to surgical biopsy, we illustrate the criteria used by radiologists for choosing the most appropriate combination of device type for sampling and imaging technique for guidance. Then, we describe the commonly used devices, from fine-needle sampling to tissue biopsy with larger needles, namely core needle biopsy and vacuum-assisted biopsy, and how mammography, digital breast tomosynthesis, ultrasound, or magnetic resonance imaging work for targeting the lesion for sampling or localisation. The differences among the techniques available for localisation (carbon marking, metallic wire, radiotracer injection, radioactive seed, and magnetic seed localisation) are illustrated. Type and rate of possible complications are described and the issue of concomitant antiplatelet or anticoagulant therapy is also addressed. The importance of pathological-radiological correlation is highlighted: when evaluating the results of any needle sampling, the radiologist must check the concordance between the cytology/pathology report of the sample and the radiological appearance of the biopsied lesion. We recommend that special attention is paid to a proper and tactful approach when communicating to the woman the need for tissue sampling as well as the possibility of cancer diagnosis, repeat tissue sampling, and or even surgery when tissue sampling shows a lesion with uncertain malignant potential (also referred to as “high-risk” or B3 lesions). Finally, seven frequently asked questions are answered.
Proximal femoral focal deficiency – a rare congenital entity: two case reports and a review of the literature
Authors: Uduma Felix U. , Dim Edwin M. , Njeze Ngozi R. 
Published: February 2020

Abstract
Background
Proximal femoral focal deficiency is an extremely rare congenital anomaly with an incidence of 1.1–2.0 in 100,000 live births. It is a dysplastic phenomenon with predilections for the proximal two-thirds of the femur leading to limb length discrepancies.
We report two cases of proximal femoral focal deficiency, which is a rare entity.

Case presentations
Case 1
A 4.5-month-old baby Annang tribe girl was referred in April 2019 to our Radiology Department, University of Uyo Teaching Hospital, Nigeria for lower limb radiographs. This was on account of her shortened left lower limb from birth despite uneventful antenatal history. An examination revealed bulky left thigh with abduction of her left hip joint.
Radiographic evaluations showed absent left femoral capital epiphysis, with deficient proximal left femur. A diagnosis of proximal femoral focal deficiency was made. Sadly, the parents and baby failed to honor future orthopedic consultations on intimation of sequential management protocols.
Case 2
A 4-month-old baby Ibibio tribe girl was similarly referred in August 2019 to the same Radiology Department for lower limb conventional radiographs due to short left lower limb that was noticed from birth. An examination showed shortened left lower limb in external rotation. Her right and left lower limbs measured 27 cm and 23 cm, respectively, with landmark taken from anterior superior iliac spine to tip of medial malleolus. A diagnosis of proximal femoral focal deficiency was made. Corroborating radiographs showed shortened and hypoplastic left femoral shaft but preserved femoral capital epiphysis. Coincidentally, the parents have not brought back their baby to our orthopedic clinic.

Conclusions
The discovery of two cases of proximal femoral focal deficiency, a rare entity, from referrals for conventional radiography in our Radiology Department encourages literature documentation. Such recognition will facilitate early institution of management, thus ensuring meaningful childhood growth.
Features extraction and analysis for device-free human activity recognition based on channel statement information in b5G wireless communications
Authors: Yuan Hui , Yang Xiaolong , He Ailin , Li Zhaoyu , Zhang Zhenya , Tian Zengshan 
Published: February 2020

AbstractFeatures extraction and analysis for human activity recognition (HAR) have been studied for decades in the 5th generation (5G) and beyond the 5th generation (B5G) era. Nowadays, with the extensive use of unmanned aerial vehicles (UAVs) in the civil field, integrating wireless signal receivers on UAVs could be a better choice to receive hearable signals more conveniently. In recent years, the HAR system based on CSI based on WiFi radar has received widespread attention due to its low cost and privacy protection property. However, in the existing CSI-based HAR system, there are two disadvantages: (1) The detection threshold is manually set, which limits its adaptability and immediacy in different wireless environments. (2) A sole classifier is used to complete the recognition, resulting in poor robustness and relatively low recognition accuracy. In this paper, we propose a CSI-based device-free HAR (CDHAR) system with WiFi-sensing radar integrated on UAVs to recognize everyday human activities. Firstly, by using machine learning, CDHAR applies kernel density estimation (KDE) to obtain adaptive detection thresholds to complete the extraction of activity duration. Second, we proposed a random subspace classifier ensemble method for classification, which applies the frequency domain feature instead of the time domain feature, and we choose each kind of feature in the same amount. Finally, we prototype CDHAR on commercial WiFi devices and evaluate its performance in both indoor environment and outdoor environments. The experiment results tell that even if experimental scenario varies, the accuracy of activity durations extraction can reach 98% and 99.60% whether in outdoor or indoor environments. According to the extracted data, the recognition accuracy in outdoor and indoor environments can reach 91.2% and 90.2%, respectively. CDHAR ensures high recognition accuracy while improving the adaptability and instantaneity.
Out-of-pocket payments and economic consequences from tuberculosis care in eastern China: income inequality
Authors: Liu Yan , Xu Cai-Hong , Wang Xiao-Mo , Wang Zhen-Yu , Wang Yan-Hong , Zhang Hui , Wang Li 
Published: February 2020

Abstract
Background
Despite the availability of free tuberculosis (TB) diagnosis and treatment, TB care still generates substantial costs that push people into poverty. We investigated out-of-pocket (OOP) payments for TB care and assessed the resulting economic burden and economic consequences for those with varying levels of household income in eastern China.

Methods
A cross-sectional study was conducted among TB patients in the national TB programme networks in eastern China. TB-related direct OOP costs, time loss, and coping strategies were investigated across households in different economic strata. Analysis of Variance was used to examine the differences in various costs, and Kruskal-Wallis tests were used to compare the difference in total costs as a percentage of annual household income.

Results
Among 435 patients, the mean OOP total costs of TB care were USD 2389.5. In the lower-income quartile, OOP payments were lower, but costs as a percentage of reported annual household income were higher. Medical costs and costs prior to treatment accounted for 66.4 and 48.9% of the total costs, respectively. The lower the household income was, the higher the proportion of medical costs to total costs before TB treatment, but the lower the proportion of medical costs patients spent in the intensive phase. TB care caused 25.8% of TB-affected households to fall below the poverty line and caused the poverty gap (PG) to increase by United States Dollar (USD) 145.6. Patients in the poorest households had the highest poverty headcount ratio (70.2%) and PG (USD 236.1), but those in moderately poor households had the largest increase in the poverty headcount ratio (36.2%) and PG (USD 177.8) due to TB care. Patients from poor households were more likely to borrow money to cope with the costs of TB care; however, there were fewer social consequences, except for food insecurity, in poor households.

Conclusions
Medical and pretreatment costs lead to high costs of TB care, especially among patients from the poorest households. It is necessary to train health system staff in general hospitals to promptly identify and refer TB patients. Pro-poor programmes are also needed to protect TB patients from the medical poverty trap.
Evolution of Cochlear implant mapping and vestibular function in a pediatric case of Labyrinthitis
Authors: Lipson Sophie , O'Shea Ross , Gibbons Susan , Zhou Guangwei , Brodsky Jacob 
Published: February 2020

Background Vestibular symptoms such as vertigo and imbalance are known to occur in some cochlear implant patients during the immediate postoperative period; however, acute vertigo in implanted children occurring remotely from the postoperative period has not been previously well-described. Case presentation A three-year-old girl with a history of bilateral sequential cochlear implantation presented with acute labyrinthitis associated with sudden onset of vertigo, balance impairment, and decline in right cochlear implant function 2 years after her most recent implant surgery. We describe her audiological and vestibular testing results during both the acute phase and following medical management and recovery. Conclusion Acute labyrinthitis should be considered when sudden onset vertigo and/or imbalance presents in children with cochlear implants outside of the perioperative period. Such symptoms should prompt early assessment of cochlear implant function, so that the device can be reprogrammed accordingly.
Cytokine pre-activation of cryopreserved xenogeneic-free human mesenchymal stromal cells enhances resolution and repair following ventilator-induced lung injury potentially via a KGF-dependent mechanism
Authors: Horie Shahd , Gaynard Sean , Murphy Mary , Barry Frank , Scully Michael , O’Toole Daniel , Laffey John G. 
Published: February 2020

AbstractBackgroundHuman mesenchymal stem/stromal cells (hMSCs) represent a promising therapeutic strategy for ventilator-induced lung injury (VILI) and acute respiratory distress syndrome. Translational challenges include restoring hMSC efficacy following cryopreservation, developing effective xenogeneic-free (XF) hMSCs and establishing true therapeutic potential at a clinically relevant time point of administration. We wished to determine whether cytokine pre-activation of cryopreserved, bone marrow-derived XF-hMSCs would enhance their capacity to facilitate injury resolution following VILI and elucidate mechanisms of action.MethodsInitially, in vitro studies examined the potential for the secretome from cytokine pre-activated XF-hMSCs to attenuate pulmonary epithelial injury induced by cyclic mechanical stretch. Later, anaesthetised rats underwent VILI and, 6 h following injury, were randomized to receive 1 × 107XF-hMSC/kg that were (i) naive fresh, (ii) naive cryopreserved, (iii) cytokine pre-activated fresh or (iv) cytokine pre-activated cryopreserved, while control animals received (v) vehicle. The extent of injury resolution was measured at 24 h after injury. Finally, the role of keratinocyte growth factor (KGF) in mediating the effect of pre-activated XF-hMSCs was determined in a pulmonary epithelial wound repair model.ResultsPre-activation enhanced the capacity of the XF-hMSC secretome to decrease stretch-induced pulmonary epithelial inflammation and injury. Both pre-activated fresh and cryopreserved XF-hMSCs enhanced resolution of injury following VILI, restoring oxygenation, improving lung compliance, reducing lung leak and improving resolution of lung structural injury. Finally, the secretome of pre-activated XF-hMSCs enhanced epithelial wound repair, in part via a KGF-dependent mechanism.ConclusionsCytokine pre-activation enhanced the capacity of cryopreserved, XF-hMSCs to promote injury resolution following VILI, potentially via a KGF-dependent mechanism.
Biomechanical diagnostics of the cornea
Authors: Esporcatte Louise Pellegrino Gomes , Salomão Marcella Q. , Lopes Bernardo T. , Vinciguerra Paolo , Vinciguerra Riccardo , Roberts Cynthia , Elsheikh Ahmed , Dawson Daniel G. , Ambrósio Renato 
Published: February 2020

AbstractCorneal biomechanics has been a hot topic for research in contemporary ophthalmology due to its prospective applications in diagnosis, management, and treatment of several clinical conditions, including glaucoma, elective keratorefractive surgery, and different corneal diseases. The clinical biomechanical investigation has become of great importance in the setting of refractive surgery to identify patients at higher risk of developing iatrogenic ectasia after laser vision correction. This review discusses the latest developments in the detection of corneal ectatic diseases. These developments should be considered in conjunction with multimodal corneal and refractive imaging, including Placido-disk based corneal topography, Scheimpflug corneal tomography, anterior segment tomography, spectral-domain optical coherence tomography (SD-OCT), very-high-frequency ultrasound (VHF-US), ocular biometry, and ocular wavefront measurements. The ocular response analyzer (ORA) and the Corvis ST are non-contact tonometry systems that provide a clinical corneal biomechanical assessment. More recently, Brillouin optical microscopy has been demonstrated to provide in vivo biomechanical measurements. The integration of tomographic and biomechanical data into artificial intelligence techniques has demonstrated the ability to increase the accuracy to detect ectatic disease and characterize the inherent susceptibility for biomechanical failure and ectasia progression, which is a severe complication after laser vision correction.
The influence of particle shape and mineralogy on the particle strength, breakage and compressibility
Authors: Zhang Xiaoyan , Baudet Beatrice A. , Yao Ting 
Published: February 2020

AbstractIt is widely accepted that both particle shape and mineralogy could have a great effect on the particle strength. However, due to the complexity of nature grains, it is difficult to distinguish the influence of particle shape from mineralogy. This paper shows measurements of the particle shape and mineralogy of carbonate sand, completely decomposed granite, Leighton Buzzard sand and pumice sand. The particle shape of carbonate sand and completely decomposed granite is similar, but the mineral of carbonate sand is carbon dioxide while the mineral of completely decomposed granite is mica and feldspar. It is found that the single particle strength of completely decomposed granite is higher than that of carbonate sand, and the particle breakage caused by one-dimensional compressing tests of completely decomposed granite is higher than that of carbonate sand. The mineralogy of both completely decomposed granite and Leighton Buzzard sand are quartz, but completely decomposed granite is more heterogeneous, result in the single particle strength of completely decomposed granite is much lower than that of Leighton Buzzard sand. It is also found the particle breakage from the one-dimensional compression test for completely decomposed is larger than Leighton Buzzard sand. It should be noted that carbonate sand is stronger than completely decomposed granite after quantitatively analysis the particle shape, particle breakage and compressibility behavior although carbonate sand has extremely irregular shape such as a branch shape, and also several pores. On the other hand, although Leighton Buzzard sand, completely decomposed granite and also pumice sand to some extent have composition of silica, the crystal of those three is completely different, result in the strength or compressibility are quite different. It seems that particle shape is not a major factor to affect strength or compressibility, but the mineralogy could be a major factor.