bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Modified Arthroscopic All-inside Repair for Lateral Meniscal Tears Anterior to the Popliteal Hiatus: A Retrospective Case Series with Minimum 2-Year Follow-Up
Authors: Zhuo Hongwu , Zhu Fugui , Pan Ling , Li Jian 
Published: April 2020

Abstract
Background﻿To determine the clinical and MRI outcomes after modified arthroscopic all-inside repair for lateral meniscal tears anterior to the popliteal hiatus.MethodsPatients who underwent modified arthroscopic all-inside repair for lateral meniscal tears anterior to the popliteal hiatus at our institution were identified. The clinical assessment consisted of the symptoms of meniscal tears, McMurray test, and patient-reported outcomes (Lysholm score, Tegner score, and International Knee Documentation Committee (IKDC) score). The status of meniscal healing was assessed using postoperative MRI scan.ResultsTwenty-five patients met the inclusion criteria. The mean age was 27.60 ± 8.37 years (range, 16 to 43 years). The mean follow-up period was 26.04 ± 2.88 months (range, 24 to 36 months). At final follow-up, the symptoms of meniscal tears disappeared in 23 patients with a negative McMurray test. The patient-reported outcomes of Lysholm, Tegner, and IKDC score improved significantly compared to the preoperative values (P = 0.001). No significant differences were observed in postoperative clinical outcomes between patients with or without concomitant ACL tears (P > 0.05). Postoperative MRI scan showed that the repaired lateral meniscus anterior to the popliteal hiatus obtained healing in 23 (92.0%) patients and no healing in 2 patients.ConclusionThis modified arthroscopic all-inside repair technique was safe and effective to treat lateral meniscal tears anterior to the popliteal hiatus, resulting in significantly improved clinical outcomes with a high healing rate and low risk of adjacent structures injury.
The Importance of Mental Models in Implementation Science
Authors: Holtrop Jodi Summers , Scherer Laura , Matlock Daniel , Glasgow Russell E , Green Lee A 
Published: April 2020

Abstract
Background: Implementation science is concerned with the study of adoption, implementation and maintenance of evidence-based interventions and use of implementation strategies to facilitate translation into practice. Ways to conceptualize and overcome challenges to implementing evidence-based practice may enhance the field of implementation science. The concept of mental models may be one way to view such challenges and to guide selection, use and adaptation of implementation strategies. Methods: A mental model is an interrelated set of beliefs that shape how a person forms expectations for the future and understands the way the world works. Mental models can shape how an individual thinks about or understands how something or someone does, can, or should function in the world. We review the concepts of mental models and illustrate how they pertain to implementation of an example intervention, shared decision making. A range of methods to elicit and analyze mental models are reviewed, including formal methods developed specifically for a given project. Results: Mental models may be sparse or detailed, may be shared among actors in implementation or not, and may be substantially tacit, i.e., of limited accessibility to introspection. Actors’ mental models can determine what information they are willing to accept and what changes they are willing to consider. We describe and illustrate multiple methods for eliciting and analyzing mental models including semi-structured interviews, cognitive task analyses and Delphi methods. Conclusions: Understanding the mental models of actors in implementation can provide crucial information for understanding, anticipating, and overcoming implementation challenges. Successful implementation often requires changing actors’ mental models or the way in which interventions or implementation strategies are presented or implemented. Accurate elicitation and understanding can guide strategies for doing so.
Agastache rugosa alleviates the multi-hit effect on hepatic lipid metabolism, inflammation and oxidative stress during nonalcoholic fatty liver disease
Authors: Cui Yizhe , Chang Renxu , Wang Qiuju , Liang Yusheng , Loor Juan J , Xu Chuang 
Published: April 2020

Abstract
Background

Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease, and has high rates of morbidity and mortality worldwide. Agastache rugosa (AR) possesses unique anti-oxidant, anti‑inflammatory and anti-atherosclerosis characteristics.
Methods

To investigate the effects and the underlying mechanism of AR on NAFLD, we fed mice a high-fat diet (HFD) to establish NAFLD model of mice in vivo experiment and induced lipidosis in AML12 hepatocytes through a challenge with free fatty acids (FFA) in vitro. The contents of total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver homogenates were measured. Pathological changes in liver tissue were evaluated by HE staining. Oil red O staining was used to determine degree of lipid accumulation in liver tissue, and Western blot was used to detect abundance of inflammation-, lipid metabolism- and endoplasmic reticulum stress-related proteins.
Results

Supply of AR alleviated accumulation of lipid in hepatocytes induced by HFD in vivo and challenged with free fatty acids (FFA) in vitro. Compared with the HFD group, supplementing AR decreased p-NF-κB/NF-κB and p-IκB/IκB protein and inhibited abundance of PERK, IRE1 and ATF6 (P < 0.05). Furthermore, AR reduced lipid accumulation within hepatocytes by downregulating abundance of SREBP, ACC1 and FAS (P < 0.05). Supply of AR significantly attenuated ROS accumulation and MDA production by improving antioxidant enzymatic activity including SOD and GSH (P < 0.01).
Conclusion

Supply of AR attenuates disordered lipid metabolism and enhances the antioxidative defense associated with NAFLD induced by HFD in mice. Results underscore the potential of plants used in traditional Chinese medicine to achieve pharmacological benefits through a multi-tier cellular response.
How effective are digital interventions in increasing flu vaccination amongst pregnant women? A systematic review protocol
Authors: Parsons Jo , Atherton Helen 
Published: April 2020

Abstract
Background: Pregnant women and their unborn babies are at increased risk of complications as a result of flu, yet uptake of the flu vaccination in the UK remains low. Digital interventions have proven effectiveness in changing health behaviour, but their effectiveness in increasing flu vaccination amongst pregnant women has not been examined. This protocol details the design and methodology of a systematic review and meta-analysis, examining the effectiveness of digital interventions in increasing flu vaccination amongst pregnant women.Methods: Bibliographic databases will be searched using appropriate search terms related to vaccination, pregnancy and flu. Randomised, non-randomised, quasi randomised controlled trials and other quantitative study designs will be eligible for inclusion, and studies will present the rate of flu vaccination amongst pregnant women of digital interventions compared to non-digital interventions, or usual care. No date or study country restrictions will be put on included studies, but studies will be published in English. Discussion: This is the first known systematic review to examine the effectiveness of digital interventions in increasing the rate of flu vaccination amongst pregnant women. This review has the potential to inform whether digital interventions are an appropriate and successful method of increasing flu vaccination amongst pregnant women, and to determine which mode of digital intervention is most effective.Registration: This systematic review is registered on the international prospective register of systematic reviews (PROSPERO). Registration number: INSERT NUMBER.
Analysis of compliance and efficacy of integrated management of whole process in the choice of percutaneous full-endoscopic surgery for patients with cervical disc herniation
Authors: Kong Weijun , Jiang Zhongyan , Wang Ansu , Wang Chong 
Published: April 2020

Abstract

Background : Percutaneous spinal endoscopy technique is a new type of surgery for the treatment of cervical disc herniation. It can avoid the complications of classic ACDF approach and the risk of adjacent spondylosis. How to effectively improve patients' awareness of spinal endoscopy and choose to receive endoscopic techniques? Objective: To analyze the compliance and clinical effect of the integrated management of whole process in the choice of percutaneous full-endoscopic surgery for patients with cervical disc herniation. Methods: Retrospective analysis of 72 patients with cervical disc herniation undergoing surgery in our hospital in August 2015~ August 2017, all of them carried out the whole process integrated management mode, 36 patients in the experimental group were treated by percutaneous full-endoscopic cervical discectomy, and 36 patients in the control group were treated by anterior cervical discectomy and fusion(ACDF). The postoperative feeding time, time to get out of bed, length of hospital stay, compliance, clinical efficacy, and recurrence rate of neck pain were observed. And changes in pain visual analogue score (VAS) and neurological function JOA score of preoperative and postoperative. Results: The postoperative feeding time in the experimental group was 8.319 ± 1.374 hours, the postoperative bedtime was 16.64 ± 3.728 hours, and the hospitalization time was 6.403 ± 0.735 days; The clinical excellent and good rate was 91.67%, the compliance rate was 88.89%, and the neck pain recurrence rate was 5.56%. The postoperative feeding time in the control group was 26.56 ± 9.512 hours, the postoperative bedtime was 45.06 ± 9.027 hours, and the length of hospital stay was 8.208 ± 0.865 days; The excellent and good rate was 88.89%, the compliance rate was 69.4%, and the neck pain recurrence rate was 8.33%. There was no significant difference between the two groups in the excellent treatment rate and the recurrence rate of neck pain, p> 0.05; The compliance rate of the experimental group was better than the control group, and the difference was statistically significant, p <0.05; the hospitalization time of the experimental group was significantly lower than that of the control group, the difference was statistically significant, p<0.05. The postoperative VAS scores and JOA scores of the two groups were significantly improved compared with preoperative, the difference was statistically significant, p<0.05; and there was no significant difference between the two groups, p> 0.05. Conclusion : The integrated management mode of the whole course can effectively improve the compliance of patients with cervical disc herniation receiving endoscopic treatment, obtain the same treatment effect as the classic operation, shorten the hospitalization time, speed up the turnover of hospital beds, improve the satisfaction of medical quality, and be worthy of clinical application.
Comparative effect of vitamin E compounds on HSP70 expression in response to acute redox imbalance in Chang liver cells
Authors: Khaza’ai Huzwah , Khorami Somayeh Alsadat Hosseini , Mutalib Mohd Sokhini Abd 
Published: April 2020

Abstract

Background: HSP70 represents the most highly induced member of the stress protein family which is constitutively expressed in normal cell conditions as chaperon and rapidly upregulated in response to stress conditions. Vitamin E compounds are considered to be the most potent oxidation chain breaking compounds. Although α-tocopherol, has been long considered as the most potent antioxidant among the vitamin E isomers, many studies have recently suggested a higher antioxidant potency of tocotrienols compared to tocopherols. Present study was carried out to investigate the effects of tocotrienol supplementation compared to α-tocopherol on HSP70 expression.Methods: To assess whether the increase in HSP70 secondary to the stress caused by the oxidant could decrease in the presence of vitamin E; two groups of cells were incubated for 4 hours with α-tocopherol and palm tocotrienol-rich fraction respectively with concentration of 10, 20 and 30 μg/ml after the cells were pre-exposed to oxidative stress.Results: The comparison between the negative control which represents the basal expression of HSP70 and the positive control that represents the expression of HSP70 as a response to the oxidant, has clearly indicated that treatment with 100 μM copper sulphates for 24 hours resulted in a significant up-regulation of HSP70 expression. The present study has shown that the overall effect of vitamin E compounds seems to reduce the increase in HSP70 expression. α-tocopherol tended to trigger a more constant rate of response to the dose given but unable to significantly induced the decrease of the HSP70 expression as compared to tocotrienol.Conclusion: Treatment with vitamin E has conferred significant protection against stress induced cellular damage. Whether the reduction of HSP70 by vitamin E is deleterious or beneficial is still controversial and needs to be considered.
Are lumbar traction strength and sit and reach flexibility associated with the development of musculoskeletal pain in two years in university staff?
Authors: Weber Vinicius Muller Reis , Fernandes Daniel Zanardini , Portela Bruno Sergio , Romanzini Marcelo , Volpato Leonardo Alex , Milla Stefanie , Pereira Wagner Menna , Vieira Edgar Ramos , Ronque Enio Ricardo Vaz , Queiroga Marcos Roberto 
Published: April 2020

Abstract
Background: Increase of musculoskeletal disorders is related to an economic burden; also elevate time spend in sedentary behavior can lead to a decrease in physical fitness and consequently an incidence in musculoskeletal disorders. The aim of the study was to evaluate if low lumbar traction strength and flexibility could predict musculoskeletal pain in university staff in two years. Methods: This is a longitudinal study with 2-year follow-up. Fifty-three university staff members participated in this longitudinal study: age = 38±8 years, height = 171±10 cm, body mass = 75±14 kg, and body mass index = 26±4 m/kg2. The participants performed the lumbar traction and the sit and reach tests, and completed the Corlett and Bishop body map and musculoskeletal pain questionnaire at baseline and two years later. Results: There was a 4% increase in pain prevalence on year 2, but 62% of the participants remained in the same pain classification. Men presented 11% decrease in lumbar traction strength, whereas women presented 21% decrease in strength. Flexibility level at baseline was not associated with developing pain. However, individuals in the lower 75th lumbar traction strength percentile at baseline had 3.2 higher odds of reporting musculoskeletal pain two years later than those in the higher 25th strength percentile. Conclusion: University staff presented decreases in lumbar traction strength and flexibility over a two year period. Weaker individuals at baseline were more likely to report musculoskeletal pain two years later than those who were stronger. Flexibility levels were not associated with developing pain.
The early intervention of geniposide and PNS combination modulated Aβ production and synaptic plasticity in young APP/PS1 transgenic mice
Authors: Tan Yan , He Fang , Li Jiao , Zhang Yali , Yi Liushu , Zhang Ce , Gu Ruoxi , Yang Ke , Zhang Jiani , Wei Peng , Wang Xu , Hua Qian 
Published: April 2020

Abstract
Background
Alzheimer’s disease (AD) is a common neurodegenerative disease with age-related, which accounts for nearly 80% of dementia. Accompanied with aging population worldwide and clinical failures in AD drugs, early prevention and diagnosis are under main concern. Traditional Chinese medicine (TCM) has a long history in the prevention of cognition declines. In clinic, the combination of geniposide and Panax notoginseng saponin (PNS) is clinically efficacious in the treatment of ischemic cerebral stroke and vascular dementia. In vivo, under a preventive strategy, the combination can improve spatial learning and memory and reduce amyloid plaques in a variety of AD-like animal models.
Methods
In order to illustrate the neuroprotective role of geniposide and PNS combination under the early intervention strategy, we medically administrated at one-month age of APP/PS1 transgenic mice for three months. The morphology of pyramidal neurons in hippocampus CA1 was observed after Hematoxylin and Eosin staining (HE staining) and the dendritic spines in hippocampus CA1 area are by Golgi silver staining. Thereafter we detect the level of Aβ1–40 and Aβ1–42 in hippocampus and in cortex by ELISA. In addition, western blotting was used for further understanding the effects of geniposide and PNS combination in APP/PS1 transgenic mice.
Results
We found that the ratio of Aβ1−42 / Aβ1−40 in both cortex and hippocampus was significantly decreased under the treatment; secondly, the combination significantly increased dendrite spine density in hippocampus CA1 areas, and increased the protein level of PSD-95 (synaptic function-related protein in post-synapse).
Conclusion
These results indicated that geniposide and PNS combination modulated Aβ production and synaptic plasticity in the early stage of AD processing.
Effect of amlodipine on cytosolic calcium and apoptosis in hyperglycemia induced-human neuronal cell line culture
Authors: KURNIAWAN SHAHDEVI NANDAR , Ciptorini Ariesia Dewi , Husna Machlusil , Rahmawati Dessika 
Published: April 2020

Abstract
Objective

Diabetic neuropathy is complication of diabetes which is believed to be caused by improper Ca2+ (calcium) signaling and activation of apoptotic process of neuron of dorsal root ganglia. Amlodipine, antagonist Ca2+, has been already known to has neuroprotective effect in vitro or in vivo. This research investigates the effect of amlodipine in cytosolic calcium level and apoptosis in SH-SY5Y human neural cell line after exposure of chronic hyperglycemia. The study design was an experimental study using human neuronal cell line SH-SY5Y, exposed by chronic hyperglycemia for 6 days with concentration of glucose 25 µM (normoglycemia) and 50 µM (hyperglycemia), then was added amlodipine 2 µM for 30 minutes.
Results

In this study, hyperglycemia increased calcium concentration and caspase-3 compared with normoglycemia (p = 0.004 and p = 0.001 respectively). There was significant difference (p = 0.015) between calcium concentration in hyperglycemia induced cell line after given amlodipine 2 µM compared without amlodipine. There was significant difference (p = 0.027) between caspase 3 level in hyperglycemia induced cell line after given amlodipine 2 µM and without amlodipine. Administration of amlodipine significantly reduced cytosolic calcium and caspase-3 level in hyperglycemia induced SH-SY5Y human neural cell lines.
How to select the fusion and fixation range of thoracolumbar and lumbar tuberculosis: A retrospective clinical study
Authors: qiang Yang zong , hao Liu chang , kui Niu ning , jing Tang , Jami Sayed Abdulla , li Wang zi , qiang Ding hui , Jiandang Shi 
Published: April 2020

Abstract
Purpose

To investigate the clinical data of thoracolumbar and lumbar spinal tuberculosis with diseased and non-diseased intervertebral surgery, evaluate the clinical efficacy of the two surgical methods, and explore how to choose the fusion of fixation range.
Methods

Among 221 patients with thoracolumbar and lumbar tuberculosis were categorized into two groups. 118 patients were in the diseased intervertebral surgery group (lesion vertebral pedicle fixation, group A) and there were 103 patients in the non-diseased intervertebral surgery group (1 or 2 vertebral fixation groups at the above and below levels of the affected vertebra, group B). Both groups of patients were treated with primary or staging, anterior combined complete lesion removal, bone graft fusion, and internal fixation. By analyzing of clinical data and the clinical efficacy of the two surgical methods in thoracolumbar and lumbar tuberculosis was evaluated.
Results

The mean follow-up duration was 65months (range 50–68 months). There were no significant differences in laboratory examination, VAS scores, and the Cobb angle correction rate and the angle loss, however, significant differences between the two groups in operation time, blood loss, drainage volume, and transfusion, the diseased intervertebral surgery group was significantly better than the non-diseased intervertebral surgery group. Meanwhile, the bone graft was fused entirely at the last follow-up.
Conclusion

Under the conditions of strictly grasping the indications for surgery, intervertebral surgery for thoracolumbar and lumbar tuberculosis is safe, effective, and feasible, and it can effectively restore its physiological curvature and reduce the degeneration of the adjacent vertebral body.
Identification of Hypoglycemia-dependent Endothelial Nitric Oxide Synthase O-GlcNAcylation Sites and Regulation of O-GlcNAcylation and Nitric Oxide Production by Hypoglycemia
Authors: he an , Hu Shupeng , Pi Qiangzhong , Guo Yongzheng , Long Yang , Luo Suxin , Xia Yong 
Published: April 2020

Abstract
BackgroundO-GlcNAcylation, an energy-sensitive post-translational modification, plays a major role in endothelial nitric oxide synthase (eNOS) activity regulation. However, the effect of hypoglycemia on eNOS O-GlcNAcylation and whether eNOS exists the novel O-GlcNAcylation sites under hypoglycemia is unknown. Hence, we endeavored to determine the effects of hypoglycemia on eNOS O-GlcNAcylation and the novel O-GlcNAcylation sites of eNOS.MethodBovine aortic endothelial cells (BAECs) and Sprague-Dawley rats were treated by hypoglycemia, and using immunoblotting to measure their eNOS O-GlcNAcylation. eNOS and transfected eNOS were purified by pull-down assay and immunoprecipitation respectively. Novel O-GlcNAcylation sites of eNOS were predicted by HPLC-MS and MS/MS Ion, and determined by immunoblotting. eNOS activity were detected by Elisa and isotope labelling method. ResultsIn BAECs and rats` thoracic aorta, hypoglycemia-associated activation of eNOS was accompanied by an increase in O-GlcNAcylation and had no effect on O-linked serine phosphorylation at residue 1179/1177. Changes in this post-translational modification were associated with increased O-GlcNAc transferase (OGT) activity, and were reversed by AMPK knockdown. Immunoblot analysis of cells expressing His-tagged wild-type human eNOS and human eNOS carrying a mutation at the Ser1177 phosphorylation site confirmed the increase in O-GlcNAcylation in response to hypoglycemia. The observed increase in O-GlcNAcylation indicated that eNOS contains novel O-GlcNAcylation sites that are activated by hypoglycemia. Immunoblot analysis of cells expressing His-tagged human eNOS carrying a mutation at Ser738 and Ser867 confirmed the increase in O-GlcNAcylation in response to hypoglycemia. Contrastingly, in His-tagged human eNOS carrying a mutation at Thr866, O-GlcNAcylation was unaffected by hypoglycemia. Differences among culture conditions were identified using two-way analysis of variance (ANOVA), one-way ANOVA, or unpaired Student’s t-test. ConclusionsHypoglycemia increases eNOS O-GlcNAcylation and activity, potentially via AMPK-OGT pathway, thereby showing the Thr866 as a novel O-GlcNAcylation site involved in hypoglycemia-mediated eNOS activation.