bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of the Short Professional Quality of Life Scale based on versions IV and V of the Professional Quality of Life Scale
Authors: Galiana Laura , Oliver Amparo , Arena Fernanda , De Simone Gustavo , Tomás José M. , Vidal-Blanco Gabriel , Muñoz-Martínez Inmaculada , Sansó Noemí 
Published: November 2020

Abstract

Background
This research presents a short version of the Professional Quality of Life (ProQOL) scale, one of the most frequently used questionnaires in the arena of applied healthcare investigation. It measures burnout (BO), compassion fatigue (CF), and compassion satisfaction (CS).

Methods
A 9-item version of the ProQOL was developed. In Study 1, this short version, which used items from version IV of the ProQOL, was administered to 817 palliative care professionals from Spain, Argentina, and Brazil. In Study 2, the same nine items, but this time from version V of the ProQOL, were administered to 296 Spanish palliative care professionals.

Results
Study 1: The Short ProQOL showed an adequate internal structure, and invariance across the countries studied (χ2(106) = 185.620 (p < 0.001), CFI = .929, RMSEA = 0.058 [0.044, 0.072], SRMR = 0.081). Argentinians showed higher levels of BO (mean difference = 0.172, p = 0.042, Cohen’s d = 0.168), whereas Brazilians showed higher levels of CF (Mean difference = 0.384, p = 0.002, Cohen’s d = 0.352). Study 2: the Short ProQOL again showed adequate internal structure and reliability (χ2(24) = 134.504 (p < 0.001); CFI = 0.953; RMSEA = 0.126 [0.106, 0.147]; SRMR = 0.063), and was related to coping with death, self-compassion, and self-care.

Conclusions
The Short ProQOL could help facilitate the application of harmonizing measurements and its use for cross-cultural comparisons and occupational health monitoring was satisfactory.
Chimeric antigen receptor T-cell therapy in glioblastoma: charging the T cells to fight
Authors: Land Craig A. , Musich Phillip R. , Haydar Dalia , Krenciute Giedre , Xie Qian 
Published: November 2020

Abstract
Glioblastoma multiforme (GBM) is the most common malignant brain cancer that invades normal brain tissue and impedes surgical eradication, resulting in early local recurrence and high mortality. In addition, most therapeutic agents lack permeability across the blood brain barrier (BBB), further reducing the efficacy of chemotherapy. Thus, effective treatment against GBM requires tumor specific targets and efficient intracranial drug delivery. With the most recent advances in immunotherapy, genetically engineered T cells with chimeric antigen receptors (CARs) are becoming a promising approach for treating cancer. By transducing T lymphocytes with CAR constructs containing a tumor-associated antigen (TAA) recognition domain linked to the constant regions of a signaling T cell receptor, CAR T cells may recognize a predefined TAA with high specificity in a non-MHC restricted manner, and is independent of antigen processing. Active T cells can travel across the BBB, providing additional advantage for drug delivery and tumor targeting. Here we review the CAR design and technical innovations, the major targets that are in pre-clinical and clinical development with a focus on GBM, and multiple strategies developed to improve CAR T cell efficacy.
Safety and immunogenicity of the quadrivalent human papillomavirus vaccine in patients with juvenile dermatomyositis: a real-world multicentre study
Authors: Grein Ingrid Herta Rotstein , Pinto Natalia Balera Ferreira , Groot Noortje , Martins Camila Bertini , Lobo Aline , Aikawa Nadia Emi , Barbosa Cassia , Terreri Maria Teresa , da Fraga Aline Coelho Moreira , de Oliveira Sheila Knupp Feitosa , Sztajnbok Flavio , Paim Marques Luciana B. , Islabão Aline Garcia , Appenzeller Simone , Bica Blanca , de Oliveira Sato Juliana , Magalhães Claudia Saad , Ferriani Virgínia , Pasmans Hella , Schepp Rutger , van der Klis Fiona , de Roock Sytze , Wulffraat Nico , Pileggi Gecilmara Salviato 
Published: November 2020

Abstract

Background
Concerns about the safety and efficacy of vaccines in patients with autoimmune diseases (AID) have led to contradictions and low vaccination coverage in this population, who are at a higher risk of infections, including by human papillomavirus (HPV). Although HPV vaccines have been recommended for immunocompromised patients, there is still a lack of data to support its use for AID patients, such as juvenile dermatomyositis (JDM) patients. The aim of this study was to assess the safety and immunogenicity of the quadrivalent HPV (qHPV) vaccine in a cohort of JDM patients.

Methods
JDM patients aged from 9 to 20 years and healthy controls (HC) were enrolled to receive a 3-dose schedule of qHPV vaccine from March/2014 to March/2016. Study visits were performed before the first dose, 1 month after the second and third doses, and 6 months after the third dose. Participants completed a diary of possible adverse events for 14 days following each dose of vaccination (AEFV). Disease activity and current therapy were analyzed at each visit for JDM patients. In addition, serum samples from all participants were collected to test antibody concentrations against HPV16 and 18 at each visit. Participant recruitment was conducted in ten Brazilian centres. From 47 eligible JDM patients and 41 HC, 42 and 35, respectively, completed the 3-dose schedule of the vaccine, given that five JDM patients and two HC had received doses prior to their inclusion in the study.

Results
The AEFVs presented by the participants were mild and in general did not differ between JDM and HC groups. No severe AEFVs were related to the vaccination. Disease activity was stable, or even improved during the follow-up. One month after the third dose of the vaccine the JDM group presented seropositivity of 100% for HPV16 and 97% for HPV18, similarly to the HC group, who presented 100% for both serotypes (p = 1.000). Six months after the third dose the seropositivity for the patient group was 94% for both HPV types.

Conclusions
The HPV vaccination in this cohort of JDM patients was safe and immunogenic. Since the seropositivity against HPV16 and 18 was very high after the 3-dose schedule, this regimen should be recommended for JDM patients.

Trial registration
Brazilian Clinical Trials Registry, number: RBR-9ypbtf. Registered 20 March 2018 – Retrospectively registered.
The global governance of antimicrobial resistance: a cross-country study of alignment between the global action plan and national action plans
Authors: Munkholm Louise , Rubin Olivier 
Published: November 2020

Abstract

Background
Antimicrobial resistance (AMR) is a growing problem worldwide in need of global coordinated action. With the endorsement of the Global Action Plan (GAP) on AMR in 2015, the 194 member states of the World Health Organization committed to integrating the five objectives and corresponding actions of the GAP into national action plans (NAPs) on AMR. The article analyzes patterns of alignment between existing NAPs and the GAP, bringing to the fore new methodologies for exploring the relationship between globally driven health policies and activities at the national level, taking income, geography and governance factors into account.

Methods
The article investigates the global governance of AMR. Concretely, two proxies are devised to measure vertical and horizontal alignment between the GAP and existing NAPs: (i) a syntactic indicator measuring the degree of verbatim overlap between the GAP and the NAPs; and (ii) a content indicator measuring the extent to which the objectives and corresponding actions outlined in the GAP are addressed in the NAPs. Vertical alignment is measured by the extent to which each NAP overlaps with the GAP. Horizontal alignment is explored by measuring the degree to which NAPs overlap with other NAPs across regions and income groups. In addition, NAP implementation is explored using the Global Database for Antimicrobial Resistance Country Self-Assessment.

Findings
We find strong evidence of vertical alignment, particularly among low-income countries and lower-middle-income countries but weaker evidence of horizontal alignment within regions. In general, we find the NAPs in our sample to be mostly aligned with the GAP’s five overarching objectives while only moderately aligned with the recommended corresponding actions. Furthermore, we see several cases of what can be termed ‘isomorphic mimicry’, characterized by strong alignment in the policies outlined but much lower levels of alignment in terms of actual implemented policies.

Conclusion
To strengthen the alignment of national AMR policies, we recommend global governance initiatives based on individualized responsibilities some of which should be legally binding. Our study provides limited evidence of horizontal alignment within regions, which implies that regional governance institutions (e.g., WHO regional offices) should primarily act as mediators between global and local demands to strengthen a global governance regime that minimizes policy fragmentation and mimicry behavior across member states.
Degradation of subchondral bone collagen in the weight-bearing area of femoral head is associated with osteoarthritis and osteonecrosis
Authors: Wu Zongyi , Wang Bingzhang , Tang Jiahao , Bai Bingli , Weng Sheji , Xie Zhongjie , Shen Zijian , Yan Deyi , Chen Liang , Zhang Jingdong , Yang Lei 
Published: November 2020

Abstract

Background
The aim of the study was to evaluate the change of subchondral bone collagen and trabecular bone in the weight-bearing area of femoral head from patients with osteoarthritis (OA) or osteonecrosis of femoral head (ONFH), and discuss the effect of collagen degradation on OA and ONFH.

Methods
Femoral heads from patients with femoral neck fracture (FNF) were collected as control group. All collected samples were divided into OA group (N = 10), ONFH group (N = 10), and FNF group (N = 10). Differences of subchondral bone collagen were compared through scanning electron microscope (SEM) observation, immunohistochemistry staining, and Masson’s trichrome staining. Alteration of subchondral bone was displayed through hematoxylin and eosin (H&E) staining and gross morphology.

Results
SEM results showed that collagen fibers in OA and ONFH group appeared to be thinner, rougher, sparser, and more wizened. Immunohistochemistry and Masson’s trichrome staining results demonstrated that the content of collagen fibers in the OA and ONFH group was obviously less than the FNF group. H&E staining results showed that trabecular bone in OA and ONFH group appeared to be thinner and ruptured. Gross morphology results showed that the degeneration and destruction of cartilage and subchondral bone in OA and ONFH group were severer than FNF group. The characteristics mentioned above in ONFH group were more apparent than OA group.

Conclusions
This study revealed that degradation of collagen fibers from subchondral bone in the weight-bearing area of femoral head was associated with OA and ONFH, which may help to find new therapeutic strategies of the diseases.
Umbilical cord-derived Wharton’s jelly for regenerative medicine applications in orthopedic surgery: a systematic review protocol
Authors: Main Benjamin J. , Valk Josiah A. , Maffulli Nicola , Rodriguez Hugo C. , Gupta Manu , Stone Ian W. , El-Amin Saadiq F. , Gupta Ashim 
Published: November 2020

Abstract

Background
Musculoskeletal injuries and conditions affect millions of individuals. These ailments are typically managed by immobilization, physiotherapy, or activity modification. Regenerative medicine has experienced tremendous growth in the past decades, especially in musculoskeletal medicine. Umbilical cord-derived Wharton’s jelly is an exciting new option for such therapies. Wharton’s jelly is a connective tissue located within the umbilical cord largely composed of mesenchymal stem cells and extracellular matrix components, including collagen, chondroitin sulfate, hyaluronic acid, and sulfated proteoglycans. Wharton’s jelly is a promising and applicable biologic source for orthopedic regenerative application.

Methods
A systematic search will be conducted in PubMed, ScienceDirect, and Google Scholar databases of English, Italian, French, Spanish, and Portuguese language articles published to date. References will be screened and assessed for eligibility by two independent reviewers as per PRISMA guidelines. Articles will be considered without exclusion to sex, activity, or age. Studies will be included if they used culture-expanded, mesenchymal stem/stromal cells of mesenchymal stem cells and/or connective tissue obtained from Wharton’s jelly. Studies will be excluded if Wharton’s jelly is not the sole experimental examined cell type. Placebos, conventional non-operative therapies including steroid injections, exercise, and NSAIDs will be compared. The study selection process will be performed independently by two reviewers using a reference software. Data synthesis and meta-analysis will be performed separately for clinical and pre-clinical studies.

Discussion
The results will be published in relevant peer-reviewed scientific journals. Investigators will present results at national or international conferences.

Trial registration
The protocol was registered on PROSPERO international prospective register of systematic reviews prior to commencement, CRD42020182487.
ARTP/EMS-combined multiple mutagenesis efficiently improved production of raw starch-degrading enzymes in Penicillium oxalicum and characterization of the enzyme-hyperproducing mutant
Authors: Gu Li-Sha , Tan Ming-Zhu , Li Shi-Huan , Zhang Ting , Zhang Qi-Qiang , Li Cheng-Xi , Luo Xue-Mei , Feng Jia-Xun , Zhao Shuai 
Published: November 2020

Abstract

Background
Application of raw starch-degrading enzymes (RSDEs) in starch processing for biofuel production can effectively reduce energy consumption and processing costs. RSDEs are generally produced by filamentous fungi, such as Penicillium oxalicum, but with very low yields, which seriously hampers industrialization of raw starch processing. Breeding assisted by random mutagenesis is an efficient way to improve fungal enzyme production.

Results
A total of 3532 P. oxalicum colonies were generated after multiple rounds of mutagenesis, by atmospheric and room-temperature plasma (ARTP) and/or ethyl methanesulfonate (EMS). Of these, one mutant A2-13 had the highest RSDE activity of 162.7 U/mL, using raw cassava flour as substrate, a yield increase of 61.1%, compared with that of the starting strain, OXPoxGA15A. RSDE activity of A2-13 further increased to 191.0 U/mL, through optimization of culture conditions. Increased expression of major amylase genes, including the raw starch-degrading glucoamylase gene, PoxGA15A, and its regulatory gene, PoxAmyR, as well as several single-nucleotide polymorphisms in the A2-13 genome, were detected by real-time reverse transcription quantitative PCR and genomic re-sequencing, respectively. In addition, crude RSDEs produced by A2-13, combined with commercial α-amylase, could efficiently digest raw corn flour and cassava flour at 40 °C.

Conclusions
Overall, ARTP/EMS-combined mutagenesis effectively improved fungal RSDE yield. An RSDE-hyperproducing mutant, A2-13, was obtained, and its RSDEs could efficiently hydrolyze raw starch, in combination with commercial α-amylase at low temperature, which provides a useful RSDE resource for future starch processing.
Sand flies (Diptera: Psychodidae) in eight Balkan countries: historical review and region-wide entomological survey
Authors: Dvorak Vit , Kasap Ozge Erisoz , Ivovic Vladimir , Mikov Ognyan , Stefanovska Jovana , Martinkovic Franjo , Omeragic Jasmin , Pajovic Igor , Baymak Devrim , Oguz Gizem , Hlavackova Kristyna , Gresova Marketa , Gunay Filiz , Vaselek Slavica , Ayhan Nazli , Lestinova Tereza , Cvetkovikj Aleksandar , Soldo Darinka Klaric , Katerinova Ivelina , Tchakarova Simona , Yılmaz Ayda , Karaoglu Begum , Iranzo Jose Risueno , Kadriaj Perparim , Velo Enkelejda , Ozbel Yusuf , Petric Dusan , Volf Petr , Alten Bulent 
Published: November 2020

Abstract
Background
Sand flies (Diptera: Psychodidae) are medically important vectors of human and veterinary disease-causing agents. Among these, the genus Leishmania (Kinetoplastida: Trypanosomatidae), and phleboviruses are of utmost importance. Despite such significance, updated information about sand fly fauna is missing for Balkan countries where both sand flies and autochtonous leishmaniases are historically present and recently re-emerging. Therefore, a review of historical data on sand fly species composition and distribution in the region was followed by a large-scale entomological survey in eight Balkan countries to provide a recent update on local sand fly fauna.

Methods
The literature search involved the period 1910–2019. The entomological survey was conducted at 1189 sampling stations in eight countries (Bulgaria, Bosnia and Herzegovina, Croatia, Kosovo, Montenegro, North Macedonia, Serbia and Slovenia), covering 49 settlements and 358 sampling sites between June and October in the years 2014 and 2016, accumulating 130 sampling days. We performed a total of 1189 trapping nights at these stations using two types of traps (light and CO2 attraction traps) in each location. Sampling was performed with a minimal duration of 6 (Montenegro) and a maximal of 47 days (Serbia) between 0–1000 m.a.s.l. Collected sand flies were morphologically identified.

Results
In total, 8490 sand fly specimens were collected. Morphological identification showed presence of 14 species belonging to genera Phlebotomus and Sergentomyia. Historical data were critically reviewed and updated with our recent findings. Six species were identified in Bosnia and Herzegovina (2 new records), 5 in Montenegro (2 new records), 5 in Croatia (2 new records), 9 in Bulgaria (5 new records), 11 in North Macedonia (1 new record), 10 in Serbia (no new records), 9 in Kosovo (3 new records) and 4 in Slovenia (no new records).

Conclusions
This study presents results of the first integrated sand fly fauna survey of such scale for the Balkan region, providing first data on sand fly populations for four countries in the study area and presenting new species records for six countries and updated species lists for all surveyed countries. Our findings demonstrate presence of proven and suspected vectors of several Leishmania species.

Graphical Abstract
Spatio-chromatic information available from different neural layers via Gaussianization
Authors: Malo Jesús 
Published: November 2020

AbstractHow much visual information about the retinal images can be extracted from the different layers of the visual pathway?This question depends on the complexity of the visual input, the set of transforms applied to this multivariate input, and the noise of the sensors in the considered layer. Separate subsystems (e.g. opponent channels, spatial filters, nonlinearities of the texture sensors) have been suggested to be organized for optimal information transmission. However, the efficiency of these different layers has not been measured when they operate together on colorimetrically calibrated natural images and using multivariate information-theoretic units over the joint spatio-chromatic array of responses.In this work, we present a statistical tool to address this question in an appropriate (multivariate) way. Specifically, we propose an empirical estimate of the information transmitted by the system based on a recent Gaussianization technique. The total correlation measured using the proposed estimator is consistent with predictions based on the analytical Jacobian of a standard spatio-chromatic model of the retina–cortex pathway. If the noise at certain representation is proportional to the dynamic range of the response, and one assumes sensors of equivalent noise level, then transmitted information shows the following trends: (1) progressively deeper representations are better in terms of the amount of captured information, (2) the transmitted information up to the cortical representation follows the probability of natural scenes over the chromatic and achromatic dimensions of the stimulus space, (3) the contribution of spatial transforms to capture visual information is substantially greater than the contribution of chromatic transforms, and (4) nonlinearities of the responses contribute substantially to the transmitted information but less than the linear transforms.
Stationary distribution of a stochastic hybrid phytoplankton model with allelopathy
Authors: Ji Weiming , Wang Zhaojuan , Hu Guixin 
Published: November 2020

Abstract
This research proposes and delves into a stochastic competitive phytoplankton model with allelopathy and regime-switching. Sufficient criteria are proffered to ensure that the model possesses a unique ergodic stationary distribution (UESD). Furthermore, it is testified that these criteria are sharp on certain conditions. Some critical functions of regime-switching on the existence of a UESD of the model are disclosed: regime-switching could lead to the appearance of the UESD. The theoretical findings are also applied to research the evolution of Heterocapsa triquetra and Chrysocromulina polylepis.
Successful treatment of severe pneumonia, pyopneumothorax with severe acute respiratory distress syndrome, and septic shock: a case report
Authors: Wang Xi , Lan Jian , Zhang Ruijie , Luo Xiaoqing 
Published: November 2020

Abstract

Background
This article reports a patient who survived severe pneumonia, pyopneumothorax with acute respiratory distress syndrome (ARDS), and septic shock, which is very difficult to treat.

Case presentation
Antibiotics, continuous renal replacement therapy (CRRT), bronchial lavage and other treatments were used to treat a patient with pneumonia, pyopneumothorax, severe ARDS and septic shock. After comprehensive treatment, the patient was successfully treated and survived for a long time.

Conclusions
There is a low successful clinical treatment rate for patients with pneumonia, pyopneumothorax with severe ARDS and septic shock. The successful treatment of this patient benefited from early and effective empirical therapy, targeted drug selection in the later stage, adequate closed thoracic drainage, repeated bronchial lavage, early CRRT, an appropriate respiratory support mode and parameter setting, immunotherapy and nutritional support therapy. This paper proposes a reference diagnosis and treatment solution for similar cases.