bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seed protein content and its relationships with agronomic traits in pigeonpea is controlled by both main and epistatic effects QTLs
Authors: Obala Jimmy , Saxena Rachit K. , Singh Vikas K. , Kale Sandip M. , Garg Vanika , Kumar C. V. Sameer , Saxena K. B. , Tongoona Pangirayi , Sibiya Julia , Varshney Rajeev K. 
Published: January 2020

AbstractThe genetic architecture of seed protein content (SPC) and its relationships to agronomic traits in pigeonpea is poorly understood. Accordingly, five F2 populations segregating for SPC and four agronomic traits (seed weight (SW), seed yield (SY), growth habit (GH) and days to first flowering (DFF)) were phenotyped and genotyped using genotyping-by-sequencing approach. Five high-density population-specific genetic maps were constructed with an average inter-marker distance of 1.6 to 3.5 cM, and subsequently, integrated into a consensus map with average marker spacing of 1.6 cM. Based on analysis of phenotyping data and genotyping data, 192 main effect QTLs (M-QTLs) with phenotypic variation explained (PVE) of 0.7 to 91.3% were detected for the five traits across the five populations. Major effect (PVE ≥ 10%) M-QTLs included 14 M-QTLs for SPC, 16 M-QTLs for SW, 17 M-QTLs for SY, 19 M-QTLs for GH and 24 M-QTLs for DFF. Also, 573 epistatic QTLs (E-QTLs) were detected with PVE ranging from 6.3 to 99.4% across traits and populations. Colocalization of M-QTLs and E-QTLs explained the genetic basis of the significant (P < 0.05) correlations of SPC with SW, SY, DFF and GH. The nature of genetic architecture of SPC and its relationship with agronomic traits suggest that genomics-assisted breeding targeting genome-wide variations would be effective for the simultaneous improvement of SPC and other important traits.
Efficient generation of thymic epithelium from induced pluripotent stem cells that prolongs allograft survival
Authors: Otsuka Ryo , Wada Haruka , Tsuji Hyuma , Sasaki Airi , Murata Tomoki , Itoh Mizuho , Baghdadi Muhammad , Seino Ken-ichiro 
Published: January 2020

AbstractThe thymus plays a significant role in establishing immunological self-tolerance. Previous studies have revealed that host immune reaction to allogeneic transplants could be regulated by thymus transplantation. However, physiological thymus involution hinders the clinical application of these insights. Here, we report an efficient generation of thymic epithelial-like tissue derived from induced pluripotent stem cells (iPSCs) and its potential to regulate immune reaction in allogeneic transplantation. We established an iPSC line which constitutively expresses mouseFoxn1gene and examined the effect of its expression duringin vitrodifferentiation of thymic epithelial cells (TECs). We found thatFoxn1expression enhances the differentiation induction of cells expressing TEC-related cell surface molecules along with upregulation of endogenousFoxn1. iPSC-derived TECs (iPSC-TECs) generated T cells in nude recipient mice after renal subcapsular transplantation. Moreover, iPSC-TEC transplantation to immuno-competent recipients significantly prolonged the survival of allogeneic skin. Our study provides a novel concept for allogeneic transplantation in the setting of regenerative medicine.
Functional evaluation and testing of a newly developed Teleost’s Fish Otolith derived biocomposite coating for healthcare
Authors: Montañez Nerly D. , Carreño Heider , Escobar Patricia , Estupiñán Hugo A. , Peña Darío Y. , Goel Saurav , Endrino Jose L. 
Published: January 2020

AbstractPolymers such as polycaprolactone (PCL) possess biodegradability, biocompatibility and affinity with other organic media that makes them suitable for biomedical applications. In this work, a novel biocomposite coating was synthesised by mixing PCL with layers of calcium phosphate (hydroxyapatite, brushite and monetite) from a biomineral called otolith extracted from Teleost fish (Plagioscion Squamosissimus) and multiwalled carbon nanotubes in different concentrations (0.5, 1.0 and 1.5 g/L). The biocomposite coating was deposited on an osteosynthesis material Ti6Al4V by spin coating and various tests such as Fourier transformation infrared spectroscopy (FTIR), Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), scratch tests, MTT reduction cytotoxicity, HOS cell bioactivity (human osteosarcoma) by alkaline phosphatase (ALP) and fluorescence microscopy were performed to comprehensively evaluate the newly developed biocoating. It was found that an increase in the concentration of carbon nanotube induced microstructural phase changes of calcium phosphate (CP) leading to the formation of brushite, monetite and hydroxyapatite. While we discovered that an increase in the concentration of carbon nanotube generally improves the adhesion of the coating with the substrate, a certain threshold exists such that the best deposition surfaces were obtained as PCL/CP/CNT 0.0 g/L and PCL/CP/CNT 0.5 g/L.
Global translational repression induced by iron deficiency in yeast depends on the Gcn2/eIF2α pathway
Authors: Romero Antonia María , Ramos-Alonso Lucía , Alepuz Paula , Puig Sergi , Martínez-Pastor María Teresa 
Published: January 2020

AbstractIron is an essential element for all eukaryotic organisms because it participates as a redox active cofactor in a wide range of biological processes, including protein synthesis. Translation is probably the most energy consuming process in cells. Therefore, one of the initial responses of eukaryotic cells to stress or nutrient limitation is the arrest of mRNA translation. In first instance, the budding yeast Saccharomyces cerevisiae responds to iron deficiency by activating iron acquisition and remodeling cellular metabolism in order to prioritize essential over non-essential iron-dependent processes. We have determined that, despite a global decrease in transcription, mRNA translation is actively maintained during a short-term exposure to iron scarcity. However, a more severe iron deficiency condition induces a global repression of translation. Our results indicate that the Gcn2-eIF2α pathway limits general translation at its initiation step during iron deficiency. This bulk translational inhibition depends on the uncharged tRNA sensing Gcn1-Gcn20 complex. The involvement of the Gcn2-eIF2α pathway in the response to iron deficiency highlights its central role in the eukaryotic response to stress or nutritional deprivation, which is conserved from yeast to mammals.
Glycofullerenes as non-receptor tyrosine kinase inhibitors- towards better nanotherapeutics for pancreatic cancer treatment
Authors: Serda Maciej , Malarz Katarzyna , Mrozek-Wilczkiewicz Anna , Wojtyniak Marcin , Musioł Robert , Curley Steven A. 
Published: January 2020

AbstractThe water-soluble glycofullerenes GF1 and GF2 were synthesized using two-step modified Bingel-Hirsch methodology. Interestingly, we identified buckyballs as a novel class of non-receptor Src kinases inhibitors. The evaluated compounds were found to inhibit Fyn A and BTK proteins with IC50 values in the low micromolar range, with the most active compound at 39 µM. Moreover, we have demonstrated that formation of protein corona on the surface of [60]fullerene derivatives is changing the landscape of their activity, tuning the selectivity of obtained carbon nanomaterials towards Fyn A and BTK kinases. The performed molecular biology studies revealed no cytotoxicity and no influence of engineered carbon nanomaterials on the cell cycle of PANC-1 and AsPC-1 cancer cell lines. Incubation with the tested compounds resulted in the cellular redox imbalance triggering the repair systems and influenced the changing of protein levels.
Features of Chronic Exertional Compartmental Syndrome of the Leg in
Elite Nordic Skiers
Authors: Calvelli Nadege , Vergès samuel , Rousseaux-Blanchi Marie-Philippe , Edouard Pascal , Guinot Michel 
Published: January 2020

AbstractThis study aimed to assess prevalence and incidence of chronic exertional
compartmental syndrome as well as functional outcomes after surgery in elite
Nordic skiers. An exhaustive list of 294 elite Nordic skiers from the French
national teams between 1994 and 2014 was analyzed through their individual
medical files in order to identify cases of chronic exertional compartmental
syndrome. Eighteen athletes had confirmed diagnosis and performed a
structured interview to identify factors associated with chronic exertional
compartmental syndrome and surgery outcomes. The prevalence was 6.1%
and the incidence 13 per 1000 skier-years. Biathletes had a higher
prevalence than cross-country skiers (OR=0.40, p=0.08).
Free-technique skiing and roller-skiing were the main conditions inducing
symptoms. All injured athletes had bilateral surgery and 94% of them
reported no more or sporadic leg pain after. Almost 90% resumed
competition at the same or higher level than prior surgery. Compare to
previous studies, the incidence rate of chronic exertional compartmental
syndrome is higher in French elite Nordic skiers. The higher prevalence in
biathletes and the trigger during free-technique skiing suggest a
contribution of this technique to this disease. This study also confirmed
that surgery was an efficient therapeutic solution without compromising
athletes’ career.
Basketball Activity Classification Based on Upper Body Kinematics and
Dynamic Time Warping
Authors: Hu Xinyao , Mo Shaorong , Qu Xingda 
Published: January 2020

AbstractBasketball activity classification can help document players’ statistics,
allow coaches, trainers and the medical team to quantitatively supervise
players’ physical exertion and optimize training strategy, and further
help prevent potential injuries. Traditionally, sports activity classification
was done by manual notational, or through multi-camera systems or motion sensing
technology. These methods were often erroneous and limited by space. This study
presents a basketball activity classification model based on Dynamic Time
Warping (DTW) and body kinematic measures. Twenty participants, including 10
experienced players and 10 novice players, were involved in an experimental
study. The experienced and novice players differed in their years of playing
basketball. Four basketball movements, including shooting, passing, dribbling,
and lay-up were classified by kinematic measures. The results indicate that the
proposed model can successfully classify different basketball movements with
high accuracy and efficiency. Specifically, with the resultant acceleration of
the hand, this model can achieve classification precision, recall, and
specificity up to 0.984, 0.983 and 0.994, respectively. Findings from this study
supported the feasibility of using DTW in real-time sports activity
classification and provided insights into the optimal sensor placement for
basketball activity classification applications.
Effectiveness of Multi-activity, High-intensity Interval Training in School-aged Children
Authors: Ketelhut Sascha , Kircher Eva , Ketelhut Sebastian R. , Wehlan Emanuel , Ketelhut Kerstin 
Published: January 2020

AbstractThe present study aimed to evaluate the effectiveness of a school-based multi-activity HIIT on aerobic fitness (AF) and hemodynamic parameters in children. 46 students were randomized into an intervention group (INT) (N=22) and a control group (CON) (N=24). Throughout a 3-month intervention period, both INT and CON participated in the regular physical education classes (PE) twice a week. Only INT received an instructed HIIT during the first 20 min of the PE. In addition to an AF-test, peripheral (pBP) and central (cBP) blood pressure, augmentation pressure (AP), and aortic pulse wave velocity (aPWV) were assessed. Significant differences in intervention effects in favor of INT were detected for AF (7.73, P=0.007), peripheral systolic BP (−6.13 mmHg, P=0.038), central systolic BP (−5.19 mmHg, P = 0.041), AP (−2.02 mmHg, P=0.013), and aPWV (−0.19 m/sec, P=0.031). The regular HITT intervention showed beneficial effects on AF, BP, and parameters of vascular stiffness already in children.
Pelvic Floor Muscle Training in Female Athletes: A Randomized
Controlled Pilot Study
Authors: Pires Telma Filipa , Pires Patricia Maria , Moreira Maria Helena , Gabriel Ronaldo Eugênio Calçadas Dias , João Paulo Vicente , Viana Sara Alexandra , Viana Rui Antunes 
Published: January 2020

AbstractThe aim of this study was to investigate the effects of pelvic floor muscles
training in elite female volleyball athletes and whether it is an effective
therapy for stress urinary incontinence. Fourteen athletes, both continent and
incontinent, between 18 and 30 years of age, were randomly assigned to an
experimental group or a control group. The experimental group received a
protocol for pelvic floor muscle training for 4 months. This consisted of three
phases: awareness/stabilization, strength training and power. The
control group was not subject to any intervention during the same period.
Measures were collected at the initial and final phase for both groups. Maximum
voluntary contractions were evaluated with a perineometer, involuntary urine
loss with a Pad test and quality of life with the King’s Health
Questionnaire. Baseline sociodemographic and anthropometric characteristics were
not significantly different. Comparing the two groups, the experimental group
improved maximum voluntary pelvic contractions (p<0.001) and reduced
urine loss (p=0.025), indicating the existence of significant
differences between groups in the variation from the initial and final phases.
The percentage of urine loss decreased in the experimental group, from
71.4–42.9%, suggesting that the protocol intervention for 16
weeks may help athletes with stress urinary incontinence.
Evaluation eines Behandlungskonzeptes für die neurologische
Anschlussrehabilitation nach Schlaganfall (Phase D): Studienprotokoll
für eine Multicenterstudie
Authors: Knapp Sebastian , Oesterle Ulrike , Kaluscha Rainer , Krischak Gert 
Published: January 2020

Zusammenfassung
Ziel der Studie Die AOK Baden-Württemberg verfolgt das Ziel einer
einrichtungsübergreifenden, hochwertigen Versorgungsqualität zur
Sicherstellung einer stabilen Gesamtversorgungssituation und der Steigerung der
Leistungstransparenz in der neurologischen Rehabilitation nach Schlaganfall. Um
dieses Ziel zu erreichen, initiierte sie das Projekt „AOK-proReha
Schlaganfall“. Die Sektion Versorgungsforschung und
Rehabilitationsforschung (SEVERA) des Universitätsklinikums Freiburg
entwickelte dazu im Auftrag der AOK Baden-Württemberg unter Beteiligung
neurologischer Rehabilitationseinrichtungen ein neues modulares
Behandlungskonzept zur Standardisierung der Behandlung von Schlaganfallpatienten
der Phase D. Die wissenschaftliche Evaluation des neuen Behandlungskonzeptes
erfolgt durch das Institut für Rehabilitationsmedizinische Forschung an
der Universität Ulm (IFR Ulm).
Methoden Die Evaluation erfolgte im Rahmen einer kontrollierten
Multicenterstudie. In die Studie wurden Schlaganfallpatienten der Phase D
eingeschlossen. Die Rekrutierung wurde in 14 neurologischen
Rehabilitationseinrichtungen in Baden-Württemberg durchgeführt.
Dabei wurden in drei Einrichtungen ausschließlich Fälle
für die Interventionsgruppe und in sechs Zentren nur Fälle
für die Kontrollgruppe rekrutiert. In fünf weiteren
Rehabilitationseinrichtungen wechselte das Therapieregime von bisherigen
klinikspezifischen Behandlungskonzepten zum neuen Behandlungskonzept. In diesen
Häusern wurden Patienten, die vor Einführung des neuen
Behandlungskonzepts entsprechend der bisherigen klinikspezifischen
Therapiekonzepte behandelt wurden, in die Kontrollgruppe eingeschlossen. Nach
Implementierung des neuen Behandlungskonzepts wurden dann Patienten für
die Interventionsgruppe rekrutiert. Primäre Zielgrößen
des Gruppenvergleichs sind die Behandlungsergebnisse, d.h. die funktionale und
kognitive Leistungsfähigkeit sowie die Fähigkeit zur
Durchführung der Aktivitäten des täglichen Lebens. Dabei
wurde die Assessmentbatterie zusammen mit einem Expertenbeirat aus den
Rehabilitationseinrichtungen entwickelt.
Ergebnisse Die Rekrutierung der historischen Kontrollgruppe in den
Rehabilitationseinrichtungen, die das neue Behandlungskonzept implementieren,
wurde im März 2018 abgeschlossen. Dabei konnten insgesamt 122 Patienten
eingeschlossen werden.
Diskussion Das Studienprotokoll beschreibt die Methodik im Zuge der
wissenschaftlichen Begleitung des Modellprojekts. Von den
Rehabilitationseinrichtungen wurde insbesondere das praxisnahe Vorgehen gut
aufgenommen. Die Studie wird klären, ob eine flächendeckende
Dissemination des neuen Behandlungskonzeptes empfohlen werden kann.
Somatosensory Evoked Potential and Transcranial Doppler Monitoring to Guide Shunting in Carotid Endarterectomy
Authors: Jeschko Johannes , Seidel Kathleen , Schucht Philippe , Bervini David , Fung Christian , Krejci Vladimir , Z'Graggen Werner , Fischer Urs , Arnold Marcel , Goldberg Johannes , Raabe Andreas , Beck Juergen 
Published: January 2020

Abstract
Objective Clamping of the internal carotid artery (ICA) during carotid endarterectomy (CEA) is a critical step. In our neurosurgical department, CEAs are performed with transcranial Doppler (TCD) and somatosensory evoked potential (SEP) monitoring with a 50% flow velocity/amplitude decrement warning criteria for shunting. The aim of our study was to evaluate our protocol with immediate neurologic deficits after surgery for the primary end point.
Methods This is a single-center retrospective cohort study of symptomatic and asymptomatic ICA stenosis patients from January 2012 to June 2015. Only those cases in which CEA was performed with both modalities (TCD and SEP) were included. The Mann-Whitney U test was applied to evaluate TCD and SEP ratios based on immediate postoperative neurologic deficits.
Results A total of 144 patients were included, 120 (83.3%) with symptomatic ICA stenosis. The primary end point was met by six patients (4.2%); all of them were patients with a symptomatic ICA stenosis. The stroke and death rate was 1.4%. Ratios of SEP amplitudes demonstrated significant differences between patients with and without an immediate postoperative neurologic deficit at the time of ICA clamping (p = 0.005), ICA clamping at 10 minutes (p = 0.044), and ICA reperfusion (p = 0.005). Ratios of TCD flow velocity showed no significant difference at all critical steps.
Conclusion In this retrospective series of simultaneous TCD and SEP monitoring during CEA surgery of predominantly symptomatic ICA stenosis patients, the stroke and death rate was 1.4%. SEP seemed to be superior to TCD in predicting the need for an intraoperative shunt and for predicting temporary postoperative deficits. Further prospective studies are needed.
Vestibular-Evoked Myogenic Potential Testing in Vestibular Localization and Diagnosis
Authors: Taylor Rachael L. , Welgampola Miriam S. , Nham Benjamin , Rosengren Sally M. 
Published: January 2020

AbstractVestibular-evoked myogenic potentials (VEMPs) are short-latency, otolith-dependent reflexes recorded from the neck and eye muscles. They are widely used in neuro-otology clinics as tests of otolith function. Cervical VEMPs are recorded from the neck muscles and reflect predominantly saccular function, while ocular VEMPs are reflexes of the extraocular muscles and reflect utricular function. They have an important role in the diagnosis of superior canal dehiscence syndrome and provide complementary information about otolith function that is useful in the diagnosis of other vestibular disorders. Like other evoked potentials, they can provide important localizing information about lesions that may occur along the VEMP pathway. This review will describe the VEMP abnormalities seen in common disorders of the vestibular system and its pathways.
Vertigo Due to Vascular Mechanisms
Authors: Kim Hyun Ah , Lee Hyung , Kim Ji-Soo 
Published: January 2020

AbstractIsolated dizziness and vertigo due to vascular mechanisms are frequently misdiagnosed as peripheral vestibulopathy or vestibular migraine. For diagnosis of strokes presenting with an acute prolonged (≥ 24 hours) vestibular syndrome, findings on clinical examination, such as HINTS (negative head impulse tests, detection of direction-changing gaze-evoked nystagmus, and presence of skew deviation), are more sensitive than findings on neuroimaging. Since HINTS alone cannot securely detect anterior inferior cerebellar artery strokes, additional attention should be paid to the patients with unexplained hearing loss in addition to acute prolonged vestibulopathy. For diagnosis of transient (< 24 hours) spontaneous vestibular syndrome due to vascular mechanisms, the presence of associated craniocervical pain and focal neurological symptoms/signs is the clue. Even without these symptoms or signs, however, vascular imaging combined with perfusion- and diffusion-weighted MRI should be performed in patients with multiple vascular risk factors or a high ABCD2 score (age, blood pressure, clinical features, duration of symptom, and presence of diabetes).
Ba(Ce,Zr)O3-based electrodes for protonic ceramic electrochemical cells: towards highly compatible functionality and triple-conducting behaviour
Authors: Kasyanova Anna V. , Tarutina Liana R. , Rudenko Anna O. , Lyagaeva Julia G. , Medvedev Dmitry A. 
Published: January 2020

Protonic ceramic fuel cells and electrolysis cells represent low- and intermediate-temperature electrochemical devices, which allow chemical-to-electrical energy conversion with very high efficiency and low environmental impact. In order to ensure the long-term operability of these devices, as well as to provide for their up-scaling, a number of existing challenges associated with chemical and thermal incompatibilities pertaining to the functional materials remain to be overcome. This work presents a comprehensive overview of new electrode materials based on barium cerate/zirconate. The structural fragments of these materials are similar to those of the proton-conducting Ba(Ce,Zr)O3electrolytes, which causes superior chemical compatibility between different functional materials. The primary emphasis of the research is on the functional properties of these materials such as chemical stability, thermal expansion behaviour and transport features. This in turn determines the electrochemical performance of the designed electrodes. In addition, the possibility of obtaining triple-conducting materials is discussed as means of designing electrodes with a high electrochemical active surface area required for the design of high-performance protonic ceramic fuel and electrolysis cells.The bibliography includes 208 references.
Controlled phage therapy by photothermal ablation of specific bacterial species using gold nanorods targeted by chimeric phages
Authors: Peng Huan , Borg Raymond E. , Dow Liam P. , Pruitt Beth L. , Chen Irene A. 
Published: January 2020

Significance
New methods for detecting and killing antibiotic-resistant, Gram-negative bacteria are of prime interest for a wide variety of applications. While phages have long been considered as potential antibacterial agents, many concerns about phage therapy stem from the fact that phages are replicating, evolvable entities whose biology is poorly understood in most cases. These concerns could be addressed by destroying the phage immediately upon use. We accomplish this by conjugating phages to gold nanorods, whose excitation by near-infrared light causes localized heating that essentially cooks nearby bacteria. Thus, the phages deliver gold nanorods to the targeted bacteria, and the nanorods destroy both bacteria and phages simultaneously. This strategy transforms phages from an evolving biological entity into a controlled, drug-like reagent.