bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Characteristics of COVID-19 and the Value of Mulbsta Scoring System in Prognosis Evaluation
Authors: Wang Chao , Luo Zhixiu , Wu Junyi , Zhou Jie , Lin Yahang , Yan Guilin , Yan Manman , Wang Xi , Yu Hanhua , Zeng Xiaoyun 
Published: June 2020

Abstract
BackgroundCOVID-19, a worldwideinfectious disease, has killed more than 420000 people, whichis extremely harmful.MethodsIn this single-center retrospective study, we included the novel coronavirus pneumonia confirmed in our hospital. This study collected the basic information andclinical examination features.ResultsThe enrolled 704 patients were affirmed infected with 2019-nCoV by the test of throat swabs. There are 334 men and 369 women, and gender, age, combined with basic diseasesare distinct in diverse disease classification (p<0.05). From the symptom analysis, the proportion of fever over 38 degrees, dyspnea, fatigue, poor appetite and other symptoms is diverse in different types of diseases (p<0.05). As the severity of the disease increases, the median lymphocyte count decreases, C-reactive protein increase, erythrocyte sedimentation rateincrease, albumin decrease, pleural effusion increase, D-Dimer and NT-proBNP increase significantly (p<0.05).As the disease severity increases, the average value of MuLBSTA score obviously ascend (p<0.05), MuLBSTA scoring system predicts novel coronavirus pneumonia patients' prognosis is still insufficient, and may require additional indicators including anorexia, fatigue, C reactive protein, etc (p<0.05).ConclusionThe MuLBSTA evaluation system has certain value for the evaluation of the disease, but it needs to be improved.
lncCRLA Enhanced&nbsp;Chemoresistance in Lung Adenocarcinoma That Underwentepithelial-mesenchymal Transition
Authors: Min Weili , Sun Liangzhang , Gao Xiao , Zhang Shuqun , Zhao Yang 
Published: June 2020

Abstract
BackgroundEMT confers increased metastatic potential andtheresistance to chemotherapiesto cancer cells.However, the precise mechanisms of EMT-related chemotherapy resistance remain unclear.MethodsAnticancer effects were determined by MTT, soft agar, cell cycle, propidium iodide and Annexin V analysis. The effect of drugs on tumor growth was assessed using patient-derived xenograft model and nude mice model.Morphology of tumor cells were observed by phase contrast microscopy. Proteins interactions were tested by western blot and coimmunoprecipitation. Immunohistochemistry and LNA ISH were performed to determine protein and lncRNA expression. Candidate lncRNA was screened out by microarray.Resultsc-Src-mediated Caspase-8 phosphorylationessential for EMT in lung adenocarcinoma cell linespreferentially occurs in cells with themesenchymal phenotype,resultingin chemoresistance tocisplatin plus paclitaxel in patients with resectable lung adenocarcinoma and a significantly worse5-yearPFS. Cisplatin killedlung adenocarcinoma cells regardless of Caspase-8. Paclitaxel-triggered necroptosis in lung adenocarcinoma cells was dependent on the phosphorylation or deficiency ofCaspase-8, during which FADDinteracted with RIPK1 to activateRIPK1/RIPK3/MLKL signaling axis. Accompanied with c-Src-mediated Caspase-8 phosphorylation to trigger EMT, a novel lncRNA named lncCRLA was markedly upregulated andinhibited RIPK1-induced necroptosis byimpairingRIPK1-RIPK3 interaction via binding to the intermediate domain of RIPK1. Dasatinibmitigatedc-Src-mediated phosphorylationof Caspase-8-induced EMT and enhanced necroptosis in mesenchymal-like lung adenocarcinoma cells treated with paclitaxel, while c-FLIP knockdown predominantly sensitized the mesenchymal-like lung adenocarcinoma cells to paclitaxel+dasatinib.Conclusionsc-Src-Caspase-8 interaction initiatesEMT and chemoresistance viaCaspase-8 phosphorylation and lncCRLA expression, to which the dasatinib/paclitaxel liposome+siFLIPregimen was lethal.
Dynamic alterations of myeloid-derived suppressor cells and CD68+CD163+M2-like macrophages of non-small cell lung cancer patients in radiotherapy
Authors: Lv Minghe , Zhuang Xibing , Shao Shali , Li Xuan , Cheng Yunfeng , Wu Duojiao , Wang Xiangdong , Qiao Tiankui 
Published: June 2020

Abstract
BackgroundRecent studies showed that myeloid-derived suppressor cells (MDSCs) and M2-like macrophages are involved in the treatment of non-small cell lung cancer (NSCLC) as immunosuppressive cells; however, the changes of MDSCs and M2-like macrophages, and their prognostic value are rarely unknown in NSCLC patients during radiotherapy. In this article, we aim to explore dynamic alteration of the circulating MDSCs and M2-like macrophages, to examine their relationship, and to evaluate their prognostic value for NSCLC patients in radiotherapy.Method:Peripheral blood mononuclear cells from healthy controls and NSCLC patients in radiotherapy were isolated to examine the circulating MDSCs and M2-like macrophages. The peripheral MDSCs defined as CD11b+CD33+HLA-DR− and M2-like Macrophages signified as CD68+CD163+ were determined by flow cytometry. 40 plasma inflammatory cytokines were measured by multiplex ELISA.ResultsCompared with health controls, the percentages of MDSCs and CD68+CD163+M2-like macrophages of NSCLC patients were significantly elevated, and were distinctly higher in NSCLC patients in radiotherapy than in pre-radiotherapy. Moreover, MDSCs correlated positively with CD68+CD163+M2-like macrophages in NSCLC patients in radiotherapy and post-radiotherapy. Interestingly, the alterations of the percentages of MDSCs and CD68+CD163+M2-like macrophages in RT were irrelated with radiotherapy area. During radiotherapy, the proportions of MDSCs were clearly increased in adenocarcinoma patients but not in squamous carcinoma patients, while the proportions of CD68+CD163+M2-like macrophages were markedly elevated in squamous carcinoma patients but not in adenocarcinoma patients. In addition, the percentages of MDSCs and CD68+CD163+ M2-like macrophages were also increased in NSCLC patients reached PR in radiotherapy compared to NSCLC patients reached SD and PD. IL-1ra and MIP-1β have a positive relation with MDSCs or M2 macrophages in NSCLC patients in radiotherapy, and Eotaxin was correlated with CD68+CD163+M2-like macrophages but not MDSCs in NSCLC patients after radiotherapy.ConclusionsThe dynamic alterations of MDSCs and M2-like macrophages, as well as their connection with plasm inflammation cytokines, could provide potentially prognostic and sensitive markers for NSCLC patients in radiotherapy.Trial Registration: Jinshan Hospital of Fudan University, IEC-2020-S01. Registered 22 May 2020.
Sinusoidal endothelial cell progenitor cells promote tumor progression in the patients with hepatocellular carcinoma.
Authors: Feng Ya-xing , Li Wei , Wen Xu-dong , Zhang Ning , Yang Zhan-yu , Liu Wei-hui 
Published: June 2020

Abstract
Objective: Since sinusoidal endothelial cell progenitor cells (SEPCs) play great roles in liver regeneration, it is necessary to elucidate that whether these SEPCs participate in tumor progression of hepatocellular carcinoma (HCC).Methods: A total of 45 patients with primary HCC, who received liver resection, were included in this study. The liver tumors were removed from patients and partial tissues were prepared to identify SEPCs by double staining of CD133/CD45 and CD133/CD31 at the same location. Blood samples were correspondingly collected to examine liver function parameters and tumor markers. The demographics and clinic-pathological characteristics of the patients were all collected for correlation analysis with SEPCs.Results: SEPCs were observed within abundant of blood vessels in HCC nodules of all the 45 patients, but no SEPCs were detected in the tumor-adjacent tissues. The number of SEPCs was correlated with the expression levels of HCC tumor markers α-fetoprotein (AFP) and CA199. There was a positive correlation between the expressions of SEPCs markers and the diameters of HCC tumors in differently differentiated specimens (P<0.01). The expressions levels of SEPCs markers in poorly differentiated HCC patients were significantly higher than those in the moderately and highly differentiated HCC patients (P<0.05).Conclusions: SEPCs are in close connection with HCC progression, and they may potentially act as prognosis and metastasis predicting index and even therapeutic candidates of HCC.
Antimicrobial Resistance of Pseudomonas Aeruginosa Isolated From Patients With Wound Infection in Ethiopia. A Systematic Review Article&nbsp;
Authors: Gebreegziabher Gebremichael , Tesfahuneygn Gebrehiwet , Gebrewahd Aderajew , Aregay Abraham 
Published: June 2020

Abstract
Background: Infectious diseases have been major cause of morbidity and mortality all over the globe. The ability of bacterial pathogens to adapt and overcoming to treat by different antibiotics has been challenging in patient management. The antimicrobial resistance rates of Pseudomonas aeruginosa are known to fluctuate extensively in different settings. Active inspection of trends in antibiotic resistance of Pseudomonas aeruginosa is essential for the selection of suitable antimicrobial agent for empirical therapy. The objective of our systematic review was to determine the national antimicrobial resistance profile of Pseudomonas aeruginosa isolated from patients with wound infection in Ethiopia. Methods: We searched the Pub Med database in July and August 2018. We used the term ‘antimicrobial resistance, Pseudomonas aeruginosa, wound and Ethiopia’ to find articles published from 2011 to 2018 September. Only articles in English language were included. Full-text articles were incorporated if they reported the percentage of antibiotic resistance among clinical isolates of pathogenic bacteria collected from patients in any of the regions of Ethiopia. For overlapping studies reporting on the same clinical isolates, same study period and place, only the study with the largest sample size was included.Results: From the 173 Pseudomonas aeruginosa isolates 99.5%, 95% of the isolates were resistant to ampicilin and Amoxicillin Clavulanic Acid respectively. From the isolates 28.8%, 29.7% and 39.6% of them were resistant to Gentamicine, ciprofloxacin and Ceftazidime respectively. Most of the isolates have limited susceptibility to ampicillin, Amoxicillin Clavulanic Acid, tetracycline, cotromoxazole, chloramphenicol and ceftriaxone.Conclusion: Antimicrobial resistance is likely to become a challenge in Ethiopia and may be exacerbated by overuse of antibiotics, the lack of oversight of antibiotic prescription, and the lack of relevant local data on antimicrobial resistance. Therefore, existing antimicrobial stewardship programmes should be strengthened or, where they are not yet in place, they should be developed and implemented in all regional referral hospitals in response to these challenges and Health research institutes. Antimicrobial surveillance also needs to be strengthened.
Effect of sugammadex on postoperative pulmonary complications in patients with respiratory dysfunction after laparoscopic gastric or intestinal surgery: A retrospective study
Authors: Ji Yiqin , Yuan Hui , Chen Yijun , Zhang Xincai , Wu Fan , Tang Wan , Lu Zihui , Huang Changshun 
Published: June 2020

Abstract
Background

Use of sugammadex is associated with fewer postoperative pulmonary complications. This study investigated the relationship between sugammadex and postoperative pulmonary complications (PPC) in specific patients with respiratory dysfunction.
Methods

The authors reviewed the electronic medical and anesthesia records of patients with respiratory dysfunction who had laparoscopic gastric or intestinal surgery at a single center between May 01, 2018, and December 31, 2019. The patients were divided into the sugammadex group and the non-sugammadex group, based on whether or not they received sugammadex. Binary logistic regression analyses were used to characterize the differences in incidence of PPC.
Results

A total of 112 patients were included, of which 46 patients (41.07%) received sugammadex. In the logistic regression analysis, the incidences of PPC were fewer in the sugammadex group. Postoperative fever [Odds Ratio (OR) 0.330; 95% Confidence Interval (CI) 0.137–0.793, p = 0.0213], postoperative ICU admission (OR 0.204; 95% CI 0.065 to 0.644, p = 0.007), cough (OR 0.143; 95% CI 0.061 to 0.333, p < 0.001), pleural effusion (all) (OR: 0.280; 95% CI 0.104 to 0.759, p = 0.012), pleural effusion (massive) (OR: 0.142; 95% CI 0.031 to 0.653, p = 0.012), and difficulty in breathing (OR: 0.111; 95% CI 0.014 to 0.849, p = 0.039) showed significant differences between the two groups.
Conclusions

Sugammadex-mediated reversal of neuromuscular blockade can reduce the PPC in patients with respiratory dysfunction.
Plasma miRNAs as Biomarkers for Amnestic Mild Cognitive Impairment and Modulation on BACE1
Authors: He Haining , Yang Huanqing , Zhang Wei , Xie Zuoquan , Zhang Minmin , Xu Hua , Liu Yuanyuan , An Na , Hong Bo , Yan Feng , Yue Ling , Wang Jinghua , Xiao Shifu , Wang Tao 
Published: June 2020

Abstract
Background: Amnestic mild cognitive impairment (aMCI) is a prodromal stage of Alzheimer’s disease with the beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) imbalance. Altered microRNAs (miRNA) associated with BACE1 may be potential biomarkers for aMCI diagnosis. The aims of this study were to find an effective diagnostic model for aMCI, and to explore the modulation of candidate miRNAs on BACE1 expression.Methods: Plasma RNA was extracted from participants enrolled in China longitudinal aging study (CLAS). MiRNA profiling was performed using microarray sequencing on a discovery set, compromising five aMCI subjects that confirmed by 18F-Flutemetamol PET scan and five normal controls (NC). Quantitative reverse transcription PCR was used to validate the expression levels of differently expressed miRNAs on an analysis set (20 aMCI subjects and 10 NC). Diagnostic capability of candidate miRNAs was assessed in a validation set (40 aMCI subjects and 40 NC). Modulation of candidate miRNAs on BACE1 was explored in rat and human hippocampal neurons in vitro through transfection of miRNA mimics or inhibitor lentivirus.Results: In discovery set, we verified 46 significantly differentially expressed miRNAs between aMCI and NC groups (P<0.05). Among these, 33 miRNAs were down-regulated and 13 miRNAs were up-regulated. In analysis set, miR-1185-2-3p, miR-1909-3p, miR-22-5p, and miR-134-3p also significantly decreased in aMCI group. Four miRNAs above and miR-107 which was found to decline in previous study were selected as potential biomarkers. A diagnostic model consists of five miRNAs above had an outstanding diagnostic accuracy (81.25%) to diagnose aMCI. Except for miR-134-3p, other four miRNAs modulated BACE1 expression and C-terminal fragments-beta production effectively in rat hippocampal neurons in vitro. Similar modulation of miR-1185-2-3p and miR-1909-3p were confirmed in human hippocampal neurons in vitro.Conclusion: A diagnostic model consists of five plasma miRNAs could be novel biomarker for aMCI diagnosis. These miRNAs might be involved in pathogenesis of AD through regulating expression of BACE1.
Construction and Investigation of circRNA-miRNA-mRNA ceRNA regulatory network in breast cancer
Authors: Wu Ming , Sang Meijie , Pan Shuo , Liu Fei , Sang Meixiang 
Published: June 2020

Abstract
Background

Circular RNAs (circRNAs) have drawn lots of attention in tumorigenesis and progression. However, circRNAs as crucial regulators in multitudinous biological processes have not been systematically identified in breast cancer (BC). Our research aims to explore novel circRNAs in BC and their mechanisms of action.

Methods

The circRNA expression profile data, as well as RNA-sequencing data of BC, were downloaded from public database, respectively. The differentially expressed circRNAs, miRNA, and mRNA were determined via fold change filtering. The competing endogenous RNAs (ceRNAs) network were established on the foundation of the relationship between circular RNAs, miRNAs and mRNAs. GO and KEGG analysis of the overlapped genes were performed to predict the potential functions and mechanisms of circRNAs in BC. The CytoHubba was used to determine the hub genes from the PPI regulatory network. Morever, we further used Kaplan–Meier plotter to perform survival analysis of these hub genes. Real-time PCR was used to validate the expression of the circRNAs in BC tissues.

Results

A total of seven differential expressed circRNAs were screened. After the predicted target miRNA and DEmiRNA were intersected, four circRNA-miRNA interactions including three circRNAs and four miRNAs were determined. Furthermore, the Venn diagram was used to intersect the predicted target genes and the downregulated differentially expressed genes, and screened 149 overlapped genes. Moreover, we constructed a PPI network, and selecting six hub genes, including DGAT2, ACSL1, ADIPOQ, LPL, LEP, PCK1. Moreover, the survival analysis results revealed that low expression of ADIPOQ, LPL, LEP were obviously correlated with poor prognosis of BC patients. The real-time PCR results demonstrated that, the levels of circ_0028899, circ_0000375, and circ_0000376 were significantly down-regulated in breast cancer tissues.

Conclusions

Our study constructed and analyzed a circRNA-associated ceRNA regulatory network and discovered that circ_0028899, circ_0000375, and circ_0000376 may function as ceRNAs to serve key roles in BC.
Medication Errors in Ethiopia: Systematic Review and Meta-Analysis
Authors: Endalamaw Aklilu , Dessie Getnet , Biresaw Henok , Belachew Amare , Amare Desalegn , Workineh Yinager , Birhanu Minyichil , Belay Getaneh Mulualem , Wondim Amare , Wubneh Chalachew Adugna , Birhanu Yeneabat , Asmare Yared , Wagnew Fasil , Minuye Biniam , Kefale Demoz , Nigussie Zelalem , Habtewold Tesfa Dejenie 
Published: June 2020

Abstract
Background: The caution of medication prescription and administration are the main physician and nursing services though there was no study to show medication error at the nation level in Ethiopia. Therefore, we estimated the national prevalence of medication errors. Methods: A systematic review of studies searched in PubMed, Scopus, African Journal of Online, and Google Scholar was done. Newcastle-Ottawa quality assessment scale was used to assess the quality status of the included studies. We employed Galbraith plot and Egger’s regression test to assess publication bias. The national prevalence of medication errors was estimated using a random-effects model meta-analysis. Moreover, subgroup analysis and meta-regression analyses were done to explore the reason of statistical heterogeneity.Results: A total of 14 studies with 5,552 administered medications and 5,661 prescription sheets were included. The overall prevalence of medication error in Ethiopia was 57.6% (95% CI: 46.2, 69.0). The pooled burden of medication administration and prescription error was 58.4% (95% CI: 51.4, 65.5) and 55.8% (95% CI: 27.0, 84.6), respectively. Omission error (38%), wrong dose (38.5%), and the wrong combination of drugs (28.7%) were highly reported types of prescription errors, whereas missed doses (57.0%), technical errors (47.0%), wrong time (35.0%), and wrong dose (30.0%) were frequently observed medication administration errors.Conclusions: Medication errors were very common in Ethiopian hospitals whereby at least one out of two medications were wrongly prescribed and administered. Our review provided a shred of up-to-date evidence for clinicians, regional, and national healthcare policymakers to appraise and improve the quality of hospitals’ inpatient care.Trail registration: The protocol is registered in the Prospero database with a registration number of CRD42019138125.
Characteristics and Risk Factors Associated with Early Mortality in Patients with Polymicrobial Bloodstream Infection: A Retrospective Clinical Study
Authors: Pang Lantian , Chen SenZhong , Ge Tiantian , Chen Chao , Xu Lichen , Wu Fengtian , Huang Chunhong , Chen Zhi 
Published: June 2020

Abstract
Background and Objective Polymicrobial bloodstream infections (PBSI) in hospitalized patients are associated with increased mortality, while few studies have characterized the clinical features in this population. This study aimed to assess the risk factors and short-term prognosis of PBSI in hospitalized patients.Materials and Methods 4066 patients with culture-positive blood were included between January 1, 2015 and December 31, 2017 in the First Affiliated Hospital of Zhejiang University School of Medicine (Hangzhou, China) in our study. 218 patients were diagnosed as PBSI. The patients were divided into two groups according to the outcome after 30-day follow-up. The number of survival group were 129, while the number of non-survival group were 89. The clinical data, identified microorganisms and severity models were compared between the two groups. A cox regression model was used to identify the risk factors of 30-day mortality in PBSI patients. Five prediction models were compared by Z-test to test the value of these models to predict outcome of PBSI.Results The patients in the non-survival group were more likely to receive inappropriate antibiotic therapy at the time of PBSI and showed more severe in systemic inflammatory. They were more likely to develop to be septic shock and to be admitted in ICU than the patients in the survival group. Inappropriate initial empirical antimicrobial therapy (HR=1.713 95% CI: 1.063-2.760, p=0.027), white blood cell (HR=1.740 95% CI: 1.002-3.020, p=0.049) and platelet (HR=2.940 95% CI: 1.754-4.930, p<0.001) were independent risk factors for 30-day mortality in PBSI patients. SOFA (AUROC=0.882, 95% CI=0.832-0.922) scores was a good prognostic scoring system for predicting short-term mortality in PBSI patients. The SOFA score was more valuable than the other four models in predicting the outcome of PBSI according to the Z-test (p<0.05).Discussion and Conclusions Inappropriate initial empirical antimicrobial therapy, white blood cell and platelet were closely associated with short-term mortality.