bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of matrix metalloproteinase‐9 gene polymorphisms in glaucoma: A hospital‐based study in Chinese patients
Authors: Zhao Fengqiong , Fan Zongshan , Huang Xuewen 
Published: November 2019

AbstractBackgroundGlaucoma is the irreversible vision loss and contributes second leading cause of blindness worldwide. Matrix metalloproteinase‐9 (MMP‐9) is involved with remodeling and destruction of extracellular matrix. Elevated MMP‐9 levels and various functional variants of MMP‐9 have been associated with glaucoma in different population. In the current investigation, we tested association of MMP‐9 common variants with different clinical categories of glaucoma in Chinese population.Materials and MethodsWe enrolled total of 396 glaucoma patients those reported to hospital comprising of 212 primary angle closure glaucoma (PACG) cases and 184 primary open‐angle glaucoma POAG patients. In addition, 329 normal individuals from similar geographical areas were enrolled as healthy controls. Five common single nucleotide polymorphisms (rs3918242, rs3918254, rs2250889, rs3918249, and rs17576) were genotyped by PCR‐RFLP. Plasma levels of MMP‐9 were quantified by ELISA.ResultsHeterozygotes (GC) and allele “G” for rs2250889 polymorphism were more frequent in PACG cases compared with healthy controls (GC: P < .0001, OR = 2.26; G: P < .0001, OR = 1.19). Similarly, heterozygous mutant and minor allele for rs3918242 polymorphism were more prevalent in POAG in comparison with healthy controls. Interestingly, distribution of rs17576 variant was statistically higher in both PACG and POAG cases than healthy controls. Furthermore, analysis of plasma MMP‐9 with MMP‐9 polymorphisms revealed significant association of rs2250889, rs3918242, and rs17576 with plasma levels of the protein.ConclusionsMMP‐9 mutants are associated with elevated plasma MMP‐9 and predisposed to development of glaucoma.
Prospects for the involvement of cancer stem cells in the pathogenesis of osteosarcoma
Authors: Izadpanah Sama , Shabani Parastoo , Aghebati‐Maleki Ali , Baghbanzadeh Amir , Fotouhi Ali , Bisadi Amir , Aghebati‐Maleki Leili , Baradaran Behzad 
Published: November 2019

AbstractOsteosarcoma (OS) is one of the most common bone tumors in children and adolescents that cause a high rate of mortality in this age group and tends to be metastatic, in spite of chemotherapy and surgery. The main reason for this can be returned to a small group of malignant cells called cancer stem cells (CSCs). OS‐CSCs play a key role in the resistance to treatment and relapse and metastasis through self‐renewal and differentiation abilities. In this review, we intend to go through the different aspects of this malignant disease, including the cancer stem cell‐phenotype, methods for isolating CSCs, signaling pathways, and molecular markers in this disease, and drugs showing resistance in treatment efforts of OS.
Long noncoding MAGI2‐AS3 promotes colorectal cancer progression through regulating miR‐3163/TMEM106B axis
Authors: Ren Hui , Li Zhi , Tang Zhengjun , Li Jun , Lang Xiaoou 
Published: November 2019

AbstractColorectal cancer (CRC), is mostly derived from normal colon epithelial cells, and has been reported to be one of most common gastrointestinal malignancies globally. An increasing number of researchers have claimed that long noncoding RNAs (lncRNAs) exert significant functions in tumor progression. Nevertheless, the function of MAGI2‐AS3 remains uncertain in CRC. The expression of MAGI2‐AS3, miR‐3163, and transmembrane protein 106B (TMEM106B) messenger RNA was examined by quantitative real‐time polymerase chain reaction. Cell apoptosis was measured by caspase‐3 activity test. Cell proliferation was tested by cell‐counting kit 8 and 5‐ethynyl‐2′‐deoxyuridine assays. Cell migration was detected by transwell assay. Western blot analysis examined the protein expression of TMEM106B. The expression of Ki‐67 was evaluated by immunohistochemistry assay. The binding capacity between miR‐3163 and MAGI2‐AS3 (or TMEM106B) was studied by radioimmunoprecipitation and luciferase reporter assays. The expression of MAGI2‐AS3 and TMEM106B was conspicuously upregulated whereas miR‐3163 presented lower expression in CRC cells. MAGI2‐AS3 deficiency facilitated cell apoptosis but hampered cell proliferation and migration. MAGI2‐AS3 combined with miR‐3163 and negatively regulated miR‐3163 expression. In addition, the administration of sh‐MAGI2‐AS3 or miR‐3163 mimics suppressed CRC cell growth in vivo. Subsequently, miR‐3163 targeted TMEM106B and the transfection of sh‐MAGI2‐AS3 or miR‐3163 mimics downregulated TMEM106B expression. Rescue assays verified that TMEM106B overexpression recovered the effects of MAGI2‐AS3 inhibition on cell apoptosis, proliferation, and migration in CRC. MAGI2‐AS3 drives CRC progression through regulating miR‐3163/TMEM106B axis. This supplies innovative insights on the investigation of molecular mechanism in CRC progression.
Performance and microbial analysis during long‐term anaerobic digestion of olive mill wastewater in a packed‐bed biofilm reactor
Authors: Enaime Ghizlane , Nettmann Edith , Berzio Stephan , Baçaoui Abdelaziz , Yaacoubi Abdelrani , Wichern Marc , Gehring Tito , Lübken Manfred 
Published: November 2019

AbstractBACKGROUNDThe treatment of olive mill wastewater (OMWW) in a packed bed biofilm reactor (PBBR) and the identification of different microorganisms involved in the digestion process is an attractive field for research.RESULTSA PBBR treating OMWW under different organic loading rates (OLRs) (0.94–9.36 gCOD/(L d)), showed high stability during an extensive time (723 days) without any sign of acidification. High volumetric biogas and methane (CH4) productions, 4.1 and 2.4 LN/(Lreactor d), respectively were registered at the highest OLR. Meanwhile, high chemical oxygen demand (COD) removal (62.5–79.7%) was obtained throughout the experiment. Phenolic compounds removal was much higher during the first steps of the experiment (75.9–84.2%), and decreased as the OLR increased to about 38%. High homogeneity of biofilm thickness and Bacteria and extracellular polymeric substance distribution were demonstrated by confocal laser scanning microscopy. Results of real‐time quantitative polymerase chain reaction (PCR) and 16S rRNA amplicon analyses showed that Bacteria, mainly consisting of Firmicutes, Proteobacteria, Bacteroidetes and Chloroflexi phylum, was about eight times more abundant than Archaea in the reactor liquid phase, while Bacteria to Archaea ratio was almost one in the biofilm. This points to a major degradation of organic substances in the liquid, while methanogenesis occurred mainly within the biofilm. In the liquid, acetoclastic methanogens were more abundant than hydrogenotrophic ones, while both groups showed likely very similar contributions in the biofilm.CONCLUSIONPromising results have been presented throughout this work providing information about the long‐term operation of PBBR‐granular activated carbon treating OMWW and analyzing, in an efficient manner, the structure and the composition of the microbial community involved in OMWW digestion. © 2019 Society of Chemical Industry
Quantitative MRI for Assessment of Treatment Outcomes in a Rabbit VX2 Hepatic Tumor Model
Authors: Keller Sarah , Chapiro Julius , Brangsch Julia , Reimann Carolin , Collettini Federico , Sack Ingolf , Savic Lynn Jeanette , Hamm Bernd , Goldberg Shraga Nahum , Makowski Marcus 
Published: November 2019

Globally, primary and secondary liver cancer is one of the most common cancer types, accounting 8.2% of deaths worldwide in 2018. One of the key strategies to improve the patient's prognosis is the early diagnosis, when liver function is still preserved. In hepatocellular carcinoma (HCC), the typical wash‐in/wash‐out pattern in conventional magnetic resonance imaging (MRI) reaches a sensitivity of 60% and specificity of 96–100%. However, in recent years functional MRI sequences such as hepatocellular‐specific gadolinium‐based dynamic‐contrast enhanced MRI, diffusion‐weighted imaging (DWI), and magnetic resonance spectroscopy (MRS) have been demonstrated to improve the evaluation of treatment success and thus the therapeutic decision‐making and the patient's outcome. In the preclinical research setting, the VX2 liver rabbit tumor, which once originated from a virus‐induced anaplastic squamous cell carcinoma, has played a longstanding role in experimental interventional oncology. Especially the high tumor vascularity allows assessing the treatment response of locoregional interventions such as radiofrequency ablation (RFA) and transcatheter arterial embolization (TACE). Functional MRI has been used to monitor the tumor growth and viability following interventional treatment. Besides promising results, a comprehensive overview of functional MRI sequences used so far in different treatment setting is lacking, thus lowering the comparability of study results. This review offers a comprehensive overview of study protocols, results, and limitations of quantitative MRI sequences applied to evaluate the treatment outcome of VX2 hepatic tumor models, thus generating a unique basis for future MRI studies and potential translation into the clinical setting.Level of Evidence: 2Technical Efficacy: Stage 1J. MAGN. RESON. IMAGING 2019. J. Magn. Reson. Imaging 2020;52:668–685.
Deep‐Learning‐Based Preprocessing for Quantitative Myocardial Perfusion MRI
Authors: Scannell Cian M. , Veta Mitko , Villa Adriana D.M. , Sammut Eva C. , Lee Jack , Breeuwer Marcel , Chiribiri Amedeo 
Published: November 2019

BackgroundQuantitative myocardial perfusion cardiac MRI can provide a fast and robust assessment of myocardial perfusion status for the noninvasive diagnosis of myocardial ischemia while being more objective than visual assessment. However, it currently has limited use in clinical practice due to the challenging postprocessing required, particularly the segmentation.PurposeTo evaluate the efficacy of an automated deep learning (DL) pipeline for image processing prior to quantitative analysis.Study TypeRetrospective.PopulationIn all, 175 (350 MRI scans; 1050 image series) clinical patients under both rest and stress conditions (135/10/30 training/validation/test).Field Strength/Sequence3.0T/2D multislice saturation recovery T1‐weighted gradient echo sequence.AssessmentAccuracy was assessed, as compared to the manual operator, through the mean square error of the distance between landmarks and the Dice similarity coefficient of the segmentation and bounding box detection. Quantitative perfusion maps obtained using the automated DL‐based processing were compared to the results obtained with the manually processed images.Statistical TestsBland–Altman plots and intraclass correlation coefficient (ICC) were used to assess the myocardial blood flow (MBF) obtained using the automated DL pipeline, as compared to values obtained by a manual operator.ResultsThe mean (SD) error in the detection of the time of peak signal enhancement in the left ventricle was 1.49 (1.4) timeframes. The mean (SD) Dice similarity coefficients for the bounding box and myocardial segmentation were 0.93 (0.03) and 0.80 (0.06), respectively. The mean (SD) error in the RV insertion point was 2.8 (1.8) mm. The Bland–Altman plots showed a bias of 2.6% of the mean MBF between the automated and manually processed MBF values on a per‐myocardial segment basis. The ICC was 0.89, 95% confidence interval = [0.87, 0.90].Data ConclusionWe showed high accuracy, compared to manual processing, for the DL‐based processing of myocardial perfusion data leading to quantitative values that are similar to those achieved with manual processing.Level of Evidence: 3Technical Efficacy Stage: 1J. Magn. Reson. Imaging 2020;51:1689–1696.
Effect of Storage Conditions and Activation on Growth Factor Concentration in Platelet‐Rich Plasma
Authors: Kim Joong Il , Bae Hyun Cheol , Park Hee Jung , Lee Myung Chul , Han Hyuk Soo 
Published: November 2019

ABSTRACTThis study aimed to evaluate growth factor concentration in platelet‐rich plasma (PRP) (leukocyte‐rich PRP) based on storage temperature, duration of storage, and method of activation. PRP samples were stored at 24℃ (room temperature group), 4℃ (refrigerator group), and −70℃ (deep‐freezer group). In each temperature, four aliquots were prepared based on the time of analysis (immediately, 1, 3, and 7 days after preparation). After storage, concentrations of platelet‐derived growth factor‐AA (PDGF‐AA), transforming growth factor‐β (TGF‐β), vascular endothelial growth factor (VEGF), insulin‐like growth factor‐1 (IGF‐1), and fibroblast growth factor‐basic (FGF‐B) were assessed with/without activation using Quantikine colorimetric sandwich immunoassay kits. PRP was activated with 10% Triton‐X for PDGF‐AA, VEGF, FGF‐B, IGF‐1 measurement and sonication for TGF‐β1 measurement. Without activation, PDGF‐AA concentration was highest on day 7 in the room temperature group. With activation, the concentration of PDGF‐AA was constant over the observation period at all temperatures. Without activation, the TGF‐β1 concentration remained negligible over the observation period at all temperatures. However, with activation, TGF‐β1 gradually increased to its highest concentration on day 7 at all temperatures. Over the observation period, VEGF and IGF‐1 concentrations were constant with and without activation at all temperatures. Without activation, FGF‐B concentration increased, with the highest concentration observed on day 7 in the deep‐freezer group. With activation, FGF‐B concentration decreased after day 1 in the room temperature group. Growth factor concentration in PRP differed significantly based on storage temperature, duration of storage, and method of activation. Appropriate storage conditions and activation are important to optimize its effects on desired clinical outcomes. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:777‐784, 2020
Effects of Immunonutrition on Chemoradiotherapy Patients: A Systematic Review and Meta‐Analysis
Authors: Zheng Xiaoya , Yu Kaili , Wang Guiyue , Liu Miao , Li Yuhang , Yu Pulin , Yang Mengyuan , Guo Nana , Ma Xiaohui , Bu Yue , Peng Yahui , Han Ci , Yu Kaijiang , Wang Changsong 
Published: November 2019

AbstractObjectiveWe conducted a systematic review to assess the effects of immunonutrition on chemoradiotherapy patients.MethodsWe searched the Cochrane Central Register of Controlled Trials, EMBASE, MEDLINE, CINAHL, and the Web of Science. We assessed the risk of bias using the Cochrane Risk of Bias tool. Our primary outcomes were the incidence of oral mucositis and diarrhea. The secondary outcomes were the incidence of esophagitis, grade ≥3 oral mucositis, grade ≥3 diarrhea, grade ≥3 esophagitis, and body weight loss.ResultsA total of 1478 patients and 27 studies were included. There were no significant differences in the incidence of oral mucositis (relative risk [RR] = 0.91; 95% confidence interval [CI], 0.79–1.05), diarrhea (RR = 0.89; 95% CI, 0.76–1.05), or esophagitis (RR = 0.55; 95% CI, 0.11–2.86) between the immunonutrition group and standard nutrition/placebo group. Nevertheless, immunonutrition significantly reduced the incidence of grade ≥3 oral mucositis (RR = 0.45; 95% CI, 0.22–0.92), grade ≥3 diarrhea (RR = 0.56; 95% CI, 0.35–0.88), grade ≥3 esophagitis (RR = 0.15; 95% CI, 0.04–0.54), and losing >5% body weight (RR = 0.34; 95% CI, 0.18–0.64).ConclusionsIn this study, immunonutrition failed to reduce the incidence rates of oral mucositis, diarrhea, or esophagitis but was conducive to significantly improving the severity of oral mucositis and diarrhea esophagitis and reducing the rate of body weight loss.
Impact of water on the hydrogen bonding between halide‐based ion‐pairs investigated by Raman scattering and density functional theory calculations
Authors: Pandey Deepak K. , Sanchora Paridhi , Rana Debkumar , Donfack Patrice , Materny Arnulf , Singh Dheeraj K. 
Published: November 2019

AbstractWe present a study of the impact of water on the size‐ and electronegativity‐dependent hydrogen bonds between cation and anion of imidazolium‐based ionic liquids (ILs) with halide anions. We have combined Raman spectroscopy with density functional theory (DFT) computations to characterize the intermolecular associations of cation–anion–water moieties. The Raman band at ~1,422 cm−1, which is assigned to the ν(N─CH2─CH3) vibration of the ethyl chain in the neat ion pairs (pure ion pairs), is blue shifted by +7, +4, and +3 cm−1 for 1‐ethyl‐3‐methylimidazolim (C2mim) cation‐based C2mim X ion pairs with X = Cl, Br, and I, respectively, in a water‐rich environment. Simultaneously, upon dilution, a new peak at ~1,438 cm−1 starts to appear at the high‐wavenumber side with continuously increasing intensity. These significant perturbations of the ν(N─CH2─CH3) vibrational mode in water environment are a clear indication of the change in the alkyl chain conformation. Further, the relative intensities of the Raman bands at ~2,946 and ~2,950 cm−1 corresponding to νs (CH2) and νas (CH3) of the ethyl chain, respectively, were significantly changed upon dilution, which also confirms the conformational change of the alkyl chain. The Raman band belonging to the C4/5─H stretch mode was significantly blue shifted by +33, +41, and +42 cm−1 going from the studied neat ion pairs having Cl, Br, and I anions to the corresponding water mixtures, respectively. Significant changes were also observed for the ν(C2─H) vibrational mode of the ion pairs in water environment; here, we observed blue shifts of +60, +51, and +50 cm−1 for ion pairs having Cl, Br, and I anions, respectively. These results confirm the weakening of the ion‐pair interaction due to the interaction with water molecules. In contrast to the ILs with halide anions, C2mim BF4 does not show any variation in the Raman peak positions upon dilution. Experimental results are nicely supported by the results of DFT calculations, and a sensible connection was established.
Efficient ab initio quantum mechanical simulations of structural stability and vibrational properties of bulk, monolayer and (n,0) nanotubes: Yttrium sesquioxide Y2O3
Authors: Larbi Tarek , El‐Kelany Khaled E. , Doll Klaus , Amlouk Mosbah 
Published: November 2019

AbstractIn this contribution, we report reliable ab initio quantum mechanical simulations of a variety of physical properties concerning yttrium sesquioxide (Y2O3) in different arrangements from the bulk, the monolayer (h‐Y2O3), to the (n,0) single‐walled nanotubes in the range from n = 6 to 32, for geometry optimization and vibrational properties. Structural parameters, phonon wavenumbers, infrared (IR) and Raman intensities, and elastic constants are computed via density functional theory (DFT/B3LYP) where the trend towards the (h‐Y2O3) monolayer for large nanotube radius is discussed. We firstly report combined experimental and computational studies on the structural and vibrational properties of the bulk Y2O3. Then, IR and Raman spectra of all arrangements are simulated via the coupled perturbed Hartree–Fock and Kohn–Sham (CPHF/KS) computational schemes. For the (n,0) Y2O3 nanotube family, two sets of IR active phonon modes in the (200–400 cm−1) and (600–900 cm−1) ranges are determined. Both of them tend smoothly with different slope, towards the optical vibrational modes of the h‐Y2O3 single layer. Three sets of active phonon bands are obtained in their Raman spectrum. The first one, in the 0–100 cm−1 range contains two phonon modes, their vibration wavenumbers tend to zero at very large tube radius and are found to be connected to the elastic constants C11 and C66 of the h‐Y2O3 monolayer as the 1D → 2D transition is approached. The second one, in 200–400 cm−1 range tends to the optical mode E′ (ν = 308 cm−1) of the monolayer. The third set, in the 600–900 cm−1 range contains two active modes, their intensities tend to zero in the limit of large nanotube without change in their vibration wavenumbers.
Antioxidant and antifungal activity of phenolic compounds and their relation to aflatoxin B1 occurrence in soybeans (Glycine max L.)
Authors: Silva Bibiana , Souza Manuel M , Badiale‐Furlong Eliana 
Published: November 2019

AbstractBACKGROUNDSoybean is widely used in food formulations; however, few studies on fungal or mycotoxin contamination have been undertaken. Free, conjugated, and bound phenolic compounds, and their antioxidant and antifungal potential, were therefore evaluated together with the occurrence of aflatoxin B1 (AFB1) in soybeans.RESULTSThe conjugated and bound phenolic soybean extracts were more efficient for the inhibition of the 2,2 diphenyl‐1‐picryl‐hydrazyl (DPPH) radical, the peroxidase enzyme, and the fungal α‐amylase enzyme. Aflatoxin B1, detected at low levels (0.96 to 1.67 ng g−1), confirmed the protective effect of soybean phenolic compounds against mycotoxigenic contamination. Principal component analysis confirmed that syringic, p‐hydroxybenzoic, p‐coumaric acids and vanillin were essential for antioxidant and antifungal activities.CONCLUSIONThis study presented new insights into the functionality of phenolic compounds in soybeans, regarding their potential to protect the crops naturally against fungal contamination, avoiding aflatoxin production, as attested by the correlations between phenolic compounds and antioxidant mechanisms. © 2019 Society of Chemical Industry
Production of diacylglycerols through glycerolysis with SBA‐15 supported Thermomyces lanuginosus lipase as catalyst
Authors: Zhao Xihong , Zhao Fenghuan , Zhong Nanjing 
Published: November 2019

AbstractBACKGROUNDIn this study, SBA‐15 was functionalized by silane coupling reagents, then lipase from Thermomyces lanuginosus (TLL) was immobilized onto the parent and the organically modified SBA‐15 for diacylglycerol (DAG) production through glycerolysis.RESULTSDiacylglycerol content of 54.77 ± 0.63%, and triacylglycerol (TAG) conversion of 77.75 ± 1.24%, were obtained from the parent SBA‐15 supported TLL‐mediated glycerolysis reaction in a solvent‐free system. However, poor performance was unexpectedly observed when co‐solvents were introduced into the reaction system. After organic modification, the functionalized SBA‐15 supported TLL samples all exhibited reasonable performance, producing DAG content over 40 wt% and TAG conversion over 70 wt%. Higher DAG content, up to 59.19 ± 1.10%, was observed from the phenyl group‐modified SBA‐15 supported TLL. The operational stability of the immobilized TLL samples in glycerolysis was also improved after organic functionalization. The phenyl group‐modified SBA‐15 supported TLL showed good reusability in the present glycerolysis reaction, and 95.21 ± 4.87% of the initial glycerolysis activity remained after five cycles of reuse.CONCLUSIONThe organic modification of SBA‐15 improved the catalytic performance of its supported TLL in glycerolysis, in terms of TAG conversion, DAG content, and reusability. © 2019 Society of Chemical Industry