bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hsa_circ_0013731 Mediated by E2F1 Inhibits Ferroptosis in Hepatocellular Carcinoma Cells by Sponging miR-877-3p and Targeting SLC7A11
Authors: Zhao Zhongwei , Song Jingjing , Zhang Dengke , Wu Fazong , Tu Jianfei , Xu Min , Ji Jiansong 
Published: July 2020

Abstract
Background: The regulatory loop between circular RNAs and microRNAs has a vital role in cell death. Ferroptosis is the form of iron-dependent cell death, which is distinct from necroptosis and apoptosis. Increasing evidences showed that ferroptosis is an important form of cell death in hepatocellular carcinoma.Methods: Real-time PCR were used to examine the expression level of circ_0013731 in hepatocellular carcinoma tissues. Edu and colony formation were performed to detect the cell proliferation. A luciferase reporter assay was used to determine the relationship between circ_0013731, miR-877-3p and SLC7A11. ChIP-qPCR assays were performed to examine the potential binding of E2F1 to the circ_0013731 promoter. Iron Assay Kit (Sigma Aldrich) was used to detect total iron or Fe2+. C11 BODIPY 581/591 staining and flow cytometer were used to examine the Lipid ROS. The role of circ_0013731 was examined in xenograft tumors model. Results: We revealed that the expression level of circ_0013731 was elevated in hepatocellular carcinoma. Moreover, E2F1 promote the transcription of circ_0013731. Overexpression of circ_0013731 promoted cell growth and inhibited ferroptosis in SMMC-7721 and QGY-7703 in vitro. miR-877-3p was proved as the direct target of circ_0013731. Then, inhibition of miR-877-3p enhanced cell growth and inhibited ferroptosis. Further mechanism research demonstrated that circ_0013731 upregulated the expression level of SLC7A11 by sponging miR-877-3p. Finally, circ_0013731 promoted HCC growth via miR-877-3p/ SLC7A11 axis in vivo.Conclusions: Our data reveal that circ_0013731 mediated by E2F1 facilitates cell growth and suppressed the ferroptosis via miR-877-3p/ SLC7A11 axis in hepatocellular carcinoma. Therefore, circ_0013731 could be acted as potential therapeutical target for hepatocellular carcinoma.
Neonatal Mortality Burden and Trends in UNHCR Refugee Camps, 2006-2017: A Retrospective Analysis
Authors: Tappis Hannah , Ramadan Marwa , Vargas Josep , Kahi Vincent , Hering Heiko , Schulte-Hillen Catrin , Spiegel Paul 
Published: July 2020

Abstract
Objective: To examine neonatal mortality burden and trends in refugee camps between 2006 and 2017.Methods: Refugee population and mortality data were exported from the United Nations High Commissioner (UNHCR) Health Information System (HIS) database. Tableau was used to export the data. Stata was used for data cleaning and statistical analysis. Neonatal mortality burdens and trends in refugee camps were analyzed and compared to national and subnational neonatal mortality rates captured by household surveys.Findings: 150 refugee camps in 21 countries were included in this study, with an average population of 1,725,433 between 2006 and 2017. A total of 663,892 live births and 3,382 neonatal deaths were captured during this period. Annual country-level refugee camp neonatal mortality rates (NMR) ranged from 12 to 56 neonatal deaths per 1,000 live births. In most countries and years where national population-based surveys are available, refugee camp NMR as reported in the UNHCR HIS was lower than that of the immediate host community.Conclusion: The UNHCR HIS provides insights into the neonatal mortality burden among refugees in camp settings and issues to consider in design and use of routine health information systems to monitor neonatal health in sub-national populations. Increased visibility of neonatal deaths and stillbirths among displaced populations can drive advocacy and inform decisions needed to strengthen health systems. Efforts to count every stillbirth and neonatal death are critical, as well as improvements to reporting systems and mechanisms for data review within broader efforts to improve the quality of neonatal care practices within and outside of health facilities.
Prognostic Values of Nucleotide Polymorphism in ARDS: A Whole Exome Sequencing Association Study
Authors: Xu Jing-Yuan , Liu Ai-Ran , Wu Zong-Sheng , Xie Jian-Feng , Qu Xiao-Xiao , Li Cai-Hua , Meng Shan-Shan , Liu Song-Qiao , Yang Cong-Shan , Liu Ling , Huang Ying-Zi , Guo Feng-Mei , Yang Yi , Qiu Haibo 
Published: July 2020

Abstract
Background Genetic locus were identified associated with ARDS outcome. Our goal was to explore the associations between genetic variants and outcome of ARDS, and the prognostic values of nucleotide polymorphism in ARDS.Methods This was a single-center, prospective trial enrolling adult ARDS patients. After baseline data were collected, blood samples were drawn to perform whole exome sequencing, and single nucleotide polymorphism (SNP) / insertion-deletion to explore the quantitative and functional associations between genetic variants and ICU outcome. Then the lung injury burden (LIB), which was defined as the ratio of nonsynonymous SNP number per megabase of DNA, was used to evaluate its value in predicting outcome of ARDS. Results A total of 105 ARDS patients were enrolled in the study, including 70 survivors and 35 nonsurvivors. Based on the analysis of a total of 65542 nonsynonymous SNP, LIB in survivors was significantly higher than nonsurvivors [1892 (1848 - 1942) /MB versus 1864 (1829 - 1910) /MB, p = 0.018], while GO analysis showed that 60 functions were correlated with ARDS outcome, KEGG enrichment analysis showed that SNP/InDels were enriched in 13 pathways. Several new SNPs were found potentially associated with ARDS outcome. Analysis of LIB was used to determine its outcome predicting ability, the area under the ROC curve of which was only 0.6103, and increase to 0.712 when combined with APACHE II score. Conclusions Genetic variants are associated with ARDS outcome; however, their prognostic value still need to be verified by larger trials.Trial registration Clinicaltrials.gov NCT02644798. Registered 20 April 2015.
The Acute Effects of 5 Fluorouracil on Skeletal Muscle Resident and Infiltrating Immune Cells in Mice
Authors: VanderVeen Brandon , Sougiannis Alexander T. , Velazquez Kandy T. , Carson James A. , Fan Daping , Murphy E. Angela 
Published: July 2020

Abstract
Background: 5 fluorouracil (5FU) has been a first-choice chemotherapy drug for several cancer types (e.g. colon, breast, head & neck); however, its efficacy is diminished by patient acquired resistance and pervasive side effects. Leukopenia is a hallmark of 5FU; however, the impact of 5FU-induced leukopenia on healthy tissue is only becoming unearthed. Recently, skeletal muscle has been shown to be impacted by 5FU in clinical and preclinical setting and weakness and fatigue remain among the most consistent complaints in cancer patients undergoing chemotherapy. Monocytes, or more specifically macrophages, are the predominate immune cell in skeletal muscle which regulate turnover and homeostasis through both the removal of damaged or old materials and coordinate repair and remodeling. Whether 5FU-induced leukopenia extends beyond circulation toimpact resident and infiltrating skeletal muscle immune cells had not been examined. The purpose of the study was to examine the acute effects of 5FU on resident and infiltrating skeletal muscle monocytes and inflammatory mediators. Methods: Male C57BL/6 mice were given a physiologically translatable dose (35mg/kg) of 5FU, or PBS, i.p. once daily for 5 days to recapitulate 1 dosing cycle. Results: Our results demonstrate that 5FU reduced circulating leukocytes, erythrocytes, and thrombocytes while inducing significant body weight loss (>5%). Flow cytometry analysis of the skeletal muscle indicated a reduction in total CD45+ immune cells with a corresponding decrease in total CD45+CD11b+ monocytes. There was a strong relationship between circulating leukocytes and skeletal muscle CD45+ immune cells. Skeletal muscle Ly6cHigh activated monocytes and M1-like macrophageswere reduced with 5FU treatment while total M2-like CD206+CD11c-macrophageswere not changed with 5FU. Interestingly, 5FU reduced bone marrow CD45+ immune cells and CD45+CD11b+ monocytes.Conclusions: Our results demonstrate that 5FU induced body weight loss and decreased skeletal muscle CD45+ immune cells in associated with a reduction in infiltrating Ly6cHigh monocytes. Interestingly, the loss of skeletal muscle immune cells occurred with bone marrow cell cycle arrest.Together our results highlight that skeletal muscle is sensitive to the cytotoxic effects of 5FU which disrupts both circulating and skeletal muscle immune cells.
Impact of β-lactam antibiotics and the delivery method on Bifidobacteria and Bacteroides populations in early infancy: A Japanese cohort study
Authors: Imoto Naruaki , Kano Chie , Aoyagi Yumi , Morita Hiroto , Amanuma Fumitaka , Maruyama Hidekazu , Hashiguchi Naoyuki , Watanabe Shin 
Published: July 2020

Abstract
Background

Intestinal flora changes dynamically in early infancy. Association between colonisation of Bifidobacterium and Bacteroides, the dominant bacterial genera in the intestinal flora 6 months after birth, and development of intestinal immunity has been suggested. Antibiotic administration to the mother is an important factor in this colonisation, but few studies have considered antibiotics taken immediately before delivery, including before Caesarean section. The association of the gut flora with the delivery method is also uncertain. Here, we performed a cohort study to determine the influence of antibiotics taken immediately before delivery on the intestinal flora in early infancy, with a focus on Bifidobacterium and Bacteroides, in 130 healthy Japanese infants.
Results

Faecal samples were collected from infants at 1, 3, and 6 months after birth (383 samples) in a hospital in Japan. The samples were analysed using a next-generation sequencer. Two β-lactam antibiotics were administered to mothers immediately before delivery: Cefazolin was used before Caesarean sections and ampicillin was used for cases with premature rupture of the membranes and in Group B Streptococcus positive cases. At all ages, Bifidobacterium and Bacteroides were dominant, and the mean combined occupancy was 60–70%. Bifidobacterium was the most dominant at all ages but its abundance was significantly lower in infants who were exposed to antibiotics at delivery, and 1 and 3 months after birth, whereas no difference was found between the delivery methods. Bacteroides were the second or third dominant bacteria at all ages but their abundance was significantly lower after Caesarean section compared with that after vaginal delivery, whereas there was no difference due to antibiotic exposure. These effects were confirmed in a linear mixed-effect model. In addition, occupancy by Bifidobacterium at 1 and 3 months and by Bacteroides at 3 months differed between infants with and without siblings.
Conclusions

Exposure to antibiotics at delivery has an important effect on Bifidobacterium, the dominant bacterial genus of the intestinal flora, at an early age in Japanese infants, whereas Bacteroides is more associated with the delivery method. The influence of siblings is also significant. The clinical significance of these results in later life requires further exploration.
Analysis of the Amplitude Changes and Baseline Drift of Respiratory Motion during Liver Stereotactic Body Radiation Therapy Based on Intra-Fraction CBCT
Authors: Zeng Lu , Wang Xin , Zhou Jidan Zhou , Gong Pan , wang Xuetao , Wu Xiaohong , Deng Zhonghua , Li Bin , Zhong Renming 
Published: July 2020

Abstract
Purpose: To evaluate the amplitude changes and baseline drift of respiratory motion in liver stereotactic body radiation therapy based on intra-fraction cone beam CT (CBCT).Materials and methods: Twenty-four liver SBRT patients underwent a four-dimensional computed tomography (4D CT) scan, inter-fraction cone-beam computed tomography (CBCT), and intra-fraction CBCT to evaluate the amplitude changes and baseline drift of respiratory motion. The amplitude changes were defined as the variations between the amplitude measured in the 4D CT and those measured with fluoroscopy in inter- and intra-fraction CBCT. The baseline drifts were defined as the difference between the liver position errors and the setup errors in inter- and intra-fraction CBCT. Manual registration of the liver contour was performed to obtain liver position errors, and bone registration was performed to obtain setup errors. Meanwhile, the correlation among the liver position errors, the relative diaphragm position, and the amplitude changes was evaluated. Results: The systematic and random errors of the baseline drifts for intra-fraction CBCT in the medial-lateral (ML), superior-inferior (SI), and anterior-posterior (AP) views were 0.99 and 1.6 mm, 2.03 and 2.46 mm, and 1.02 and 2.07 mm, respectively. The corresponding PTV margin was 3.61 mm, 6.8 mm, and 4.00 mm, respectively. The amplitude variation ranged from -169.33% to 65.47% in inter-fraction CBCT and from -171.04% to 60.00% in intra-fraction CBCT. Inter-fraction liver position errors were significantly related to the setup errors. The relative diaphragm position was not statistically related to the baseline drifts in the inter-fraction nor in the intra-fraction. Conclusions: Using intra-fraction CBCT, significant amplitude variations and baseline drifts of respiratory motion were found compared with those in 4D CT. The relative diaphragm position in a single breathing cycle cannot reflect the liver position during dose delivery. At least a 6.8 mm PTV margin in the SI direction is recommended to compensate for the baseline drift.
Upfront Brain Radiotherapy Improves Intracranial Progression-Free Survival but not Overall Survival in Lung Adenocarcinoma Patients with Brain Metastases: A Retrospective Single-Institutional Analysis from China
Authors: Jiang Aijun , Ni Meng , Li Li , Zhao Fen , Yao Yuanhu , Ding Xin , Yu Qingxi , Zhang Longzhen , Yuan Shuanghu 
Published: July 2020

Abstract
Aims: There is no consensus on the optimal opportunity of brain radiotherapy (BRT) for the patients with lung adenocarcinoma and brain metastases (BM). In the present study, we performed a retrospective review to investigate the differential benefit of upfront BRT for lung adenocarcinoma patients with BM.Methods: A total of 354 lung adenocarcinoma patients with BM from the Affiliated Cancer Hospital of Shandong University met inclusion criteria for the study. Patients were divided into two groups: upfront BRT and deferred BRT. Intracranial progression-free survival (iPFS) and Overall survival (OS) were measured from the date of brain metastases. Subgroup analyses according to gene mutation status were also performed.Results: For the entire cohort, the median iPFS of upfront BRT and deferred BRT was 16.3 and11.3 months, respectively (p =.001). The median OS of upfront BRT and deferred BRT was 27.6 and 31.5 months, respectively (p =0.813). Subgroup Analyses indicated that upfront BRT yielded a significantly longer iPFS than deferred BRT (p = 0.003) only for patients with no sensitive gene mutation. In all subgroups, the median OS was not significant different for upfront BRT and deferred BRT.Conclusion: This single-institutional retrospective showed that in patients with lung adenocarcinoma and BM, upfront BRT was associated with significantly longer iPFS, but no OS difference between upfront BRT and deferred BRT was observed. Considering the reported neurocognitive toxicities of upfront BRT, deferred BRT might be considered as an acceptable therapeutic option for the treatment of patients with lung adenocarcinoma and BM.
Effect of Elaeis Guineensis Biomass Residues in the Production of Coal Pellets
Authors: ONOCHIE UCHE , EGWARE HENRY , ONOROH FRANCIS 
Published: July 2020

Abstract
In this study, the effect of elaeis guineensis in the production of pellets from coal was investigated. Coal and elaeis guineensis were collected and pulverised. A locally fabricated screw press machine was used to produce three types of pellets in the same ratio (i.e. 80C:20R) while the fourth pellet is100% coal. These pellets are: coal palm kernel shell (CPKS), coal palm fibre (CPF), coal empty fruit bunch (CEFB) as well as raw coal (C). Thereafter, the pellets were sundried and characterised base on ASTM Standards. These include the calorific value, proximate and ultimate analysis. From the results, it was observed that the calorific value of CPKS, CPF, CEFB and C were 28033.38 kJ/kg, 27695.4 kJ/kg, 27687.5 kJ/kg and 22021.99 kJ/kg respectively. The sulphur content of the pellets is 0.7%, 0.71%, 0.73% and 0.76% respectively. The results revealed that the 100% coal pellet has the lowest percentage CV and the highest percentage sulphur and ash content. Essentially, this study has been able to established that elaeis guineensis residues is a good energy source for enhancing the calorific value of coal and also has the tendency of reducing the sulphur and ash contents of coal especially the PKS.
Ketamine-propofol (Ketofol) on Procedural Sedation and Analgesia in Children: A Systematic Review and Meta-Analysis
Authors: Foo Tze Yong , Noor Norhayati Mohd , Yazid Mohd Boniami , Fauzi Mohd Hashairi , Wahab Shaik Farid Abdull , Ahmad Mohammad Zikri 
Published: July 2020

Abstract
ABSTRACT
Objectives
This review is to evaluate the efficacy and side effects of ketofol in comparison to other anaesthetic agents during procedural sedation and analgesia.
Method
The Cochrane Central Register of Controlled Trials and MEDLINE were searched, including the reference list of related randomized control trials and reviewed articles in order to find unpublished trials or trials not identified by electronic searches. Included criteria specifically comparing recovery time, clinician satisfaction and adverse effect of ketofol.
Results
Eleven trials that met our criteria were included in the analysis with total 1274 patients. Five trials compared with single agent, six trials compared with combined agents. For comparison between ketofol and single agent (ketamine or propofol), ketofol shows significant effect on recovery time (MD -9.88, 95% CI: -14.30 to -5.46; P=0.0003; I 2 = 92%) but no difference when compared to combined agents (RR 0.75, 95% CI: -6.24 to 7.74; P<0.001; I 2 = 98%). In single agent comparison, ketofol show no differences in clinician satisfaction (RR 2.86, 95% CI: 0.64 to 12.69; P=0.001; I 2 = 90%), airway obstruction (RR 0.72, 95% CI: 0.35 to 11.48; P=0.81; I 2 = 0%), apnea (RR 0.9, 95% CI: 0.33 to 2.44; P=0.88; I 2 = 0%), desaturation (RR 1.11, 95% CI: 0.64 to 1.94; P=0.28; I 2 = 21%), nausea (RR 0.52, 95% CI: 0.91 to 1.41; P=0.2; I 2 = 38%) and vomiting (RR 0.63, 95% CI: 0.25 to 1.61; P=0.18; I 2 = 42%). For comparison between ketofol and combined agents, ketofol is effective in reducing hypotension (RR 4.2, 95% CI: 0.2 to 0.85; P=0.76; I 2 = 0%) but no differences in bradycardia (RR 0.70, 95% CI: 0.14 to 03.63; P=0.09; I 2 = 53%), desaturation (RR 1.9, 95% CI: 0.15 to 23.6; P=0.11; I 2 = 61%) and respiratory depression (RR 1.98, 95% CI: 0.18 to 21.94; P=0.12; I 2 = 59%).
Conclusions
There is low certainty evidence that ketofol improve the recovery time and reduce the frequency of hypotension with moderate certainty of evidence. No difference in other adverse effect when compared to either single or combined agents.
Association between Thyroid Stimulating Hormone and Respiratory Distress Syndrome of Newborn in the Preterm Infants
Authors: Kim Young Jin , Kim Byoung Kook , Kim Yong Hyuk 
Published: July 2020

Abstract
Background: Various hormones are known to influence the production and secretion of pulmonary surfactant. But the relationship between respiratory distress syndrome (RDS) and thyroid hormone has yet to be clarified. Methods: 126 infants with gestational age between 24 and 34 weeks who were hospitalized at the neonatal ICU of the Wonju Severance Christian Hospital from April 2017 to February 2019 were included in the study. Infants were divided into 3 groups by gestational age – 24 weeks 0 days to 28 weeks 0 days, 28 weeks 0 days to 31 weeks 0 days, and 31 weeks 0 days to 33 weeks 0 days, each with 18, 34, and 74 subjects, respectively. Among the subjects, there were 56 infants with RDS and 70 infants without RDS.Results: The group with lowest gestational age showed T3 and fT4 level that was lower than those of other groups (p<0.05) on the day of birth but there was no difference in the TSH level (p=0.129). T3 and TSH level were lower in the RDS group compared with the control group on the day of birth (p<0.05). Free thyroxine (fT4) level was higher in the control group on the day of birth but without any significant difference. Multiple logistic regression analysis showed that lower serum TSH levels on the day of birth was associated with a higher incidence of RDS (p<0.05).Conclusion: The incidence of RDS was significantly higher in infants with lower serum TSH levels at birth, but there was no significant difference in RDS incidence according to serum thyroid hormone levels.
Long Noncoding RNA DNAH17-AS1 Promotes Tumorigenesis and Metastasis of Non-Small Cell Lung Cancer via Regulating miR-877-5p/CCNA2 Pathway
Authors: Du Li-juan , Mao Long-jun , Jing Rui-jun 
Published: July 2020

Abstract
Background: A growing number of studies have revealed that long noncoding RNAs (lncRNAs) can function as important oncogenes or tumor suppressors. This study aimed to investigate the regulatory role of lncRNA DNAH17 antisense RNA 1 (DNAH17-AS1) on non-small cell lung cancer (NSCLC) and the underlying molecular mechanisms.Methods: RT-PCR was used to examine the expression of DNAH17-AS1, miR-877-5p and CCNA2 in NSCLC specimens and cell lines. The diagnostic and prognostic values of DNAH17-AS1 expression in NSCLC patients were statistically analyzed. We also evaluated the effects of DNAH17-AS1 on the proliferation, migration, invasion and apoptosis of H1299 and 95D cells. Bioinformatic analysis and luciferase reporter assays were carried to confirm the molecular binding.Results: The expression of DNAH17-AS1 and CCNA2 mRNA was distinctly upregulated in NSCLC specimens and cell lines, while miR-877-5p expression was significantly decreased. DNAH17-AS1 could be used to distinguish NSCLC specimens from adjacent non-tumor tissues. Clinical assays revealed that high DNAH17-AS1 was associated with TNM stage, distant metastasis and shorter overall survival and disease-free survival. Functional assays indicated that knockdown of DNAH17-AS1 suppressed the proliferation, migration and invasion of H1299 and 95D cells, and promoted apoptosis. Mechanically, DNAH17-AS1 served as competing endogenous RNA (ceRNA) for miR-877-5p to positively recover CCNA2.Conclusion: We identified a novel NSCLC-related lncRNA, DNAH17-AS1 which may exert an oncogenic function via serving as a sponge for miR-877-5p to upregulate CCNA2. Our study presents novel insights into NSCLC progression and provided a prospective therapeutic target for NSCLC.