bluuebunny/crossref_metadata_2025_split huggingface dataset:
bFGF blockade reduces intraplaque angiogenesis and macrophage infiltration in atherosclerotic vein graft lesions in ApoE3*Leiden mice
Authors: Parma Laura , Peters Hendrika A. B. , Sluiter Thijs J. , Simons Karin H. , Lazzari Paolo , de Vries Margreet R. , Quax Paul H. A. 
Published: September 2020

AbstractIntraplaque angiogenesis increases the chance of unstable atherosclerotic plaque rupture and thrombus formation leading to myocardial infarction. Basic Fibroblast Growth Factor (bFGF) plays a key role in angiogenesis and inflammation and is involved in the pathogenesis of atherosclerosis. Therefore, we aim to test K5, a small molecule bFGF-inhibitor, on remodelling of accelerated atherosclerotic vein grafts lesions in ApoE3*Leiden mice. K5-mediated bFGF-signalling blockade strongly decreased intraplaque angiogenesis and intraplaque hemorrhage. Moreover, it reduced macrophage infiltration in the lesions by modulating CCL2 and VCAM1 expression. Therefore, K5 increases plaque stability. To study the isolated effect of K5 on angiogenesis and SMCs-mediated intimal hyperplasia formation, we used an in vivo Matrigel-plug mouse model that reveals the effects on in vivo angiogenesis and femoral artery cuff model to exclusively looks at SMCs. K5 drastically reduced in vivo angiogenesis in the matrigel plug model while no effect on SMCs migration nor proliferation could be seen in the femoral artery cuff model. Moreover, in vitro K5 impaired endothelial cells functions, decreasing migration, proliferation and tube formation. Our data show that K5-mediated bFGF signalling blockade in hypercholesterolemic ApoE3*Leiden mice reduces intraplaque angiogenesis, haemorrhage and inflammation. Therefore, K5 is a promising candidate to stabilize advanced atherosclerotic plaques.
The effects of common variants in MDM2 and GNRH2 genes on the risk and survival of osteosarcoma in Han populations from Northwest China
Authors: Feng Weilou , Wang Zhi , Feng Dongxu , Zhu Yangjun , Zhang Kun , Huang Wei 
Published: September 2020

AbstractAccumulating evidence has shown that both MDM2 and GNRH2 might be related to Osteosarcoma (OS) susceptibility. The study aimed to evaluate the effects of common variants in MDM2 and GNRH2 genes on the risk and survival of osteosarcoma in Han populations from Northwest China. In the study, we recruited 2292 subjects including 596 OS patients and 1696 healthy controls and genotyped 16 selected tag SNPs (6 from GNRH2 and 10 from MDM2). Genetic association analyses were performed at the genotypic and allelic levels. Survival curves were made for OS patients with different genotypes. Two SNPs, rs1690916 (MDM2, P = 0.0002) and rs3761243 (GNRH2, P = 0.0004), were identified to be significantly associated with OS risk. Moreover, SNP rs3761243 was strongly associated with pathological fracture (P = 2.61 × 10–14), metastasis (P < 2.2 × 10–16), and Enneking stage (P < 2.2 × 10–16) in the OS group. Furthermore, survival curves based on different genotypes of SNP rs3761243 were found to be significantly different (P = 0.0003), suggesting increased risk with more copies of C alleles. Our results provide supportive evidence for genetic associations of MDM2 and GNRH2 genes with susceptibility to OS, and for the positive correlation of SNP rs3761243 in GNRH2 with the survival status of OS patients in Han populations from Northwest China.
Therapeutic angiogenesis using autologous adipose-derived regenerative cells in patients with critical limb ischaemia in Japan: a clinical pilot study
Authors: Katagiri Takeshi , Kondo Kazuhisa , Shibata Rei , Hayashida Ryo , Shintani Satoshi , Yamaguchi Shukuro , Shimizu Yuuki , Unno Kazumasa , Kikuchi Ryosuke , Kodama Akio , Takanari Keisuke , Kamei Yuzuru , Komori Kimihiro , Murohara Toyoaki 
Published: September 2020

AbstractAdipose-derived regenerative cell (ADRC) is a promising alternative source of autologous somatic stem cells for the repair of damaged tissue. This study aimed to assess the safety and feasibility of autologous ADRC implantation for therapeutic angiogenesis in patients with critical limb ischaemia (CLI). A clinical pilot study—Therapeutic Angiogenesis by Cell Transplantation using ADRCs (TACT-ADRC) study—was initiated in Japan. Adipose tissue was obtained by ordinary liposuction method. Isolated ADRCs were injected into the ischaemic limb. We performed TACT-ADRC procedure in five patients with CLI. At 6 months, no adverse events related to the TACT-ADRC were observed. No patients required major limb amputation, and ischaemic ulcers were partly or completely healed during the 6-month follow-up. In all cases, significant clinical improvements were seen in terms of rest pain and 6-min walking distance. Numbers of circulating CD34+ and CD133+ cells markers of progenitor cell persistently increased after ADRC implantation. The ratio of VEGF-A165b (an anti-angiogenic isoform of VEGF) to total VEGF-A in plasma significantly decreased after ADRC implantation. In vitro experiments, cultured with ADRC-conditioned media (CM) resulted in increased total VEGF-A and decreased VEGF-A165b in C2C12 cells, but not in macrophages. ADRC-CM also increased CD206+ cells expression and decreased TNF-α in macrophages. Autologous ADRC implantation was safe and effective in patients with CLI and could repair damaged tissue via its ability to promote angiogenesis and suppress tissue inflammation.
H2O2 and Engrailed 2 paracrine activity synergize to shape the zebrafish optic tectum
Authors: Amblard Irène , Thauvin Marion , Rampon Christine , Queguiner Isabelle , Pak Valeriy V. , Belousov Vsevolod , Prochiantz Alain , Volovitch Michel , Joliot Alain , Vriz Sophie 
Published: September 2020

AbstractAlthough a physiological role for redox signaling is now clearly established, the processes sensitive to redox signaling remains to be identified. Ratiometric probes selective for H2O2 have revealed its complex spatiotemporal dynamics during neural development and adult regeneration and perturbations of H2O2 levels disturb cell plasticity and morphogenesis. Here we ask whether endogenous H2O2 could participate in the patterning of the embryo. We find that perturbations of endogenous H2O2 levels impact on the distribution of the Engrailed homeoprotein, a strong determinant of midbrain patterning. Engrailed 2 is secreted from cells with high H2O2 levels and taken up by cells with low H2O2 levels where it leads to increased H2O2 production, steering the directional spread of the Engrailed gradient. These results illustrate the interplay between protein signaling pathways and metabolic processes during morphogenetic events.
Individual subunits of a rhinovirus causing common cold exhibit largely different protein-RNA contact site conformations
Authors: Blaas Dieter 
Published: September 2020

AbstractRhinoviruses cause the common cold. They are icosahedral, built from sixty copies each of the capsid proteins VP1 through VP4 arranged in a pseudo T = 3 lattice. This shell encases a ss(+) RNA genome. Three-D classification of single and oligomeric asymmetric units computationally excised from a 2.9 Å cryo-EM density map of rhinovirus A89, showed that VP4 and the N-terminal extension of VP1 adopt different conformations within the otherwise identical 3D-structures. Analysis of up to sixty classes of single subunits and of six classes of subunit dimers, trimers, and pentamers revealed different orientations of the amino acid residues at the interface with the RNA suggesting that local asymmetry is dictated by disparities of the interacting nucleotide sequences. The different conformations escape detection by 3-D structure determination of entire virions with the conformational heterogeneity being only indicated by low density. My results do not exclude that the RNA follows a conserved assembly mechanism, contacting most or all asymmetric units in a specific way. However, as suggested by the gradual loss of asymmetry with increasing oligomerization and the 3D-structure of entire virions reconstructed by using Euler angles selected in the classification of single subunits, RNA path and/or folding likely differ from virion to virion.
Rehabilitation nach Schlaganfall: Durch Gehirn-Computer-Schnittstelle
vermittelte funktionelle Elektrostimulation
Authors: Krueger Johanna , Reichert Christoph , Dürschmid Stefan , Krauth Richard , Vogt Susanne , Huchtemann Tessa , Lindquist Sabine , Lamprecht Juliane , Sailer Michael , Heinze Hans-Jochen , Hinrichs Hermann , Sweeney-Reed Catherine M. 
Published: September 2020

ZusammenfassungEine Gehirn-Computer-Schnittstelle (BCI) in der Rehabilitation von
Schlaganfallpatienten ermöglicht die Steuerung einer funktionellen
Elektrostimulation (FES), um eine Muskelkontraktion in der gelähmten
Extremität zum Zeitpunkt der Bewegungsintention durch Erkennung
entsprechender Hirnsignale auszulösen. Es wird angenommen, dass eine
genaue zeitliche Kohärenz zwischen Bewegungsintention und visuellem
sowie propriozeptivem Feedback, ausgelöst durch eine reale Bewegung,
neuroplastische Prozesse begünstigen und eine funktionelle
Verbesserung der Parese bewirken kann. In dieser systematischen
Übersichtsarbeit zu randomisierten kontrollierten Studien wurden die
Datenbanken Pubmed, Scopus und Web of Science durchsucht und von 516
berücksichtigten Publikationen 13 ausgewählt, die auf 7
Studienpopulationen basierten. Ein direkter Vergleich der Studien ist durch
Unterschiede im Studiendesign erschwert. Fünf Studien berichten von
einer verbesserten motorischen Funktion in der BCI-FES-Gruppe, davon zeigen
3 signifikante Unterschiede zwischen der BCI-FES- und der
Kontrollgruppe.
Rational oder spontan?
Authors: Hertlein Margit 
Published: September 2020

ZusammenfassungWir denken pausenlos, egal, ob schnell oder langsam. Um schnell zu entscheiden, ist der Strudelwurm eine feine Sache. Er hilft uns, rasch Antworten zu finden, oft die richtigen, aber leider nicht immer. Lesen Sie in dieser Ausgabe mehr über Denk- und Bewertungssysteme.
Die Zukunft auf dem Radar
Authors: Rossius Sabrina , Andresen Philipp 
Published: September 2020

ZusammenfassungEntscheidungen zu transportieren, sie umzusetzen und mit Leben zu füllen, liegt in der Verantwortung des mittleren Pflegemanagements. Wie Sie Ihre Leitungen bei dieser schwierigen Aufgabe unterstützen können, den Zusammenhalt fördern und dabei viele neue Erkentnnise gewinnen, lesen Sie hier.
Dry arthroscopic 4-corner arthrodesis and other refinements to stay within a tourniquet time
Authors: Ruas Jaime S. , del Pinal Francisco , Mazarrasa Raquel 
Published: September 2020

Abstract4-corner arthrodesis is a widely implanted procedure to treat degenerated joints in the wrist such as SLAC and SNAC stage II and III. Arthroscopy in combination with headless cannulated screws, permits reproducing the same intervention with the advantages of a minimally invasive surgery. This technique has already been published in the past supported by the early experience in this new exciting field. The purpose of this paper is to present new technical refinements collected over the years in order to speed up the surgery while obtaining optimum results. In this paper we also present extreme scenarios solved by combining the scope with cannulated screws.
Development of a novel scoring system based on endoscopic appearance for management of rectal neuroendocrine tumors
Authors: Chen Luohai , Guo Yu , Zhang Yixuan , Liu Man , Zhang Yu , Lin Yuan , Chen Min-hu , Chen Jie 
Published: September 2020

Abstract
Background The clinical significance of the endoscopic appearance of rectal neuroendocrine tumors (NETs) is poorly understood. We aimed to develop a novel scoring system based on endoscopic appearances to predict endoscopically advanced disease in patients with rectal NETs when initially diagnosed.
Methods Patients diagnosed with well-differentiated rectal NETs between January 2005 and December 2019 were retrospectively included. Logistic regression analyses were applied to study the relationship between endoscopic appearance and advanced disease. The whole dataset was randomly divided into training and validation sets, which were used to develop and validate a novel scoring system, respectively.
Results 309 patients were included. The endoscopic appearance of rectal NETs was significantly associated with advanced disease (P < 0.001). A novel scoring system was developed based on endoscopic appearance, including tumor size, tumor shape, and mucosal surface, using the training set. The area under curve (AUC) of the scoring system to predict advanced disease was 0.953 (95 % confidence interval [CI] 0.915 – 0.991; P < 0.001) and 0.960 (95 %CI 0.905 – 1.000; P < 0.001) in the training and validation sets, respectively. Furthermore, the scoring system was significantly associated with tumor grade. Patients with high scores had significantly worse disease-free and overall survival than patients with low scores (P < 0.001).
Conclusion This novel scoring system based on the endoscopic appearance of the primary tumor can help to accurately identify patients with endoscopically advanced disease who are not suitable for endoscopic resection. In addition, it is of great value in monitoring tumor recurrence and overall survival in patients with rectal NETs.
Enantioselective Synthesis of Difluoroalkylated Isoindolinones via Chiral Spirocyclic Phosphoric Acid Catalyzed Mannich-Type Reaction
Authors: Lin Xufeng , Wang Lei , Zhong Jialing 
Published: September 2020

AbstractAn enantioselective Mannich-type reaction of in situ generated cyclic ketimines with difluoroenoxysilanes catalyzed by chiral spirocyclic phosphoric acid has been developed. This methodology provides a facile route to difluoroalkyl-substituted chiral isoindolinones bearing a quaternary stereogenic center in high yields and up to 96% enantioselectivity.
History Page: Leaders in MSK Radiology
Authors: Van Tiggelen René , Dirckx Jan 
Published: September 2020

AbstractMilitary physician Etienne Henrard has played an important role in both civil and military radiology. He was adjunct at the radiological department, created in 1897, in the military hospital in Brussels. In 1900, he equipped one of the first private cabinets in Brussels. He was especially interested in bone radiology, stereo-radiography and the localization of foreign bodies for which he invented, during the “Great War,” a device for their extraction. In 1905, he was one of the founding members of the Belgian Radiological Society where he also held many functions. In 1931, he was promoted to the rank of Physician-General.
t
BuO2H/Cu(acac)2-Mediated Intramolecular Oxidative Lactonization of o-Allyl Arylaldehydes: Synthesis of 1-Oxoisochromans
Authors: Chang Meng-Yang , Lai Kai-Xiang , Chen Kuan-Ting 
Published: September 2020

AbstractA concise route for the
t
BuO2H/Cu(acac)2-mediated synthesis of 1-oxoisochromans is described. This includes: (i) oxidation of oxygenated o-allyl arylaldehydes and (ii) sequential intramolecular lactonization of the resulting olefin-containing benzoic acids. A plausible mechanism is proposed and discussed.
An Efficient Synthesis of Functionalized 2H-1,3,5-Oxadiazines via Metal-Carbenoid-Induced 1,2,4-Oxadiazole Ring Cleavage
Authors: Novikov Mikhail S. , Strelnikova Julia O. , Rostovskii Nikolai V. , Khoroshilova Olesya V. , Khlebnikov Alexander F. 
Published: September 2020

AbstractA high-yielding method for the synthesis of 2H-1,3,5-oxadiazines by rhodium(II)- or copper(II)-catalyzed reaction of 1,2,4-oxadiazoles with α-diazo esters has been developed. The reaction proceeds via attack of the metallocarbenoid on the oxadiazole N2 atom followed by ring opening/1,6-electrocyclization and enables the introduction of alkyl, aryl, oxy, and amino substituents into the 6-position and electron-withdrawing groups into the 2-position of 1,3,5-oxadiazine. The N2-attack and the N4-attack of the carbenoid cause different oxadiazole ring openings, which are controlled by the substitution at C5. The presence of a substituent at this position is a prerequisite for the N2-attack to occur, leading to the formation of 1,3,5-oxadiazines.
Quantitative Imaging in Inflammatory Arthritis: Between Tradition and Innovation
Authors: Giraudo Chiara , Kainberger Franz , Boesen Mikael , Trattnig Siegfried 
Published: September 2020

AbstractRadiologic imaging is crucial for diagnosing and monitoring rheumatic inflammatory diseases. Particularly the emerging approach of precision medicine has increased the interest in quantitative imaging. Extensive research has shown that ultrasound allows a quantification of direct signs such as bone erosions and synovial thickness. Dual-energy X-ray absorptiometry and high-resolution peripheral quantitative computed tomography (CT) contribute to the quantitative assessment of secondary signs such as osteoporosis or lean mass loss. Magnetic resonance imaging (MRI), using different techniques and sequences, permits in-depth evaluations. For instance, the perfusion of the inflamed synovium can be quantified by dynamic contrast-enhanced imaging or diffusion-weighted imaging, and cartilage injury can be assessed by mapping (T1ρ, T2). Furthermore, the increased metabolic activity characterizing the inflammatory response can be reliably assessed by hybrid imaging (positron emission tomography [PET]/CT, PET/MRI). Finally, advances in intelligent systems are pushing forward quantitative imaging. Complex mathematical algorithms of lesions' segmentation and advanced pattern recognition are showing promising results.
Quantitative Imaging of Body Composition
Authors: Hemke Robert , Buckless Colleen , Torriani Martin 
Published: September 2020

AbstractBody composition refers to the amount and distribution of lean tissue, adipose tissue, and bone in the human body. Lean tissue primarily consists of skeletal muscle; adipose tissue comprises mostly abdominal visceral adipose tissue and abdominal and nonabdominal subcutaneous adipose tissue. ﻿Hepatocellular and myocellular lipids are also fat pools with important metabolic implications. Importantly, body composition reflects generalized processes such as increased adiposity in obesity and age-related loss of muscle mass known as sarcopenia.In recent years, body composition has been extensively studied quantitatively to predict overall health. Multiple imaging methods have allowed precise estimates of tissue types and provided insights showing the relationship of body composition to varied pathologic conditions. In this review article, we discuss different imaging methods used to quantify body composition and describe important anatomical locations where target tissues can be measured.
Identifying Musculoskeletal Pain Generators Using Clinical PET
Authors: Yoon Daehyun , Kogan Feliks , Gold Garry E. , Biswal Sandip 
Published: September 2020

AbstractIdentifying the source of a person's pain is a significant clinical challenge because the physical sensation of pain is believed to be subjective and difficult to quantify. The experience of pain is not only modulated by the individual's threshold to painful stimuli but also a product of the person's affective contributions, such as fear, anxiety, and previous experiences. Perhaps then to quantify pain is to examine the degree of nociception and pro-nociceptive inflammation, that is, the extent of cellular, chemical, and molecular changes that occur in pain-generating processes. Measuring changes in the local density of receptors, ion channels, mediators, and inflammatory/immune cells that are involved in the painful phenotype using targeted, highly sensitive, and specific positron emission tomography (PET) radiotracers is therefore a promising approach toward objectively identifying peripheral pain generators. Although several preclinical radiotracer candidates are being developed, a growing number of ongoing clinical PET imaging approaches can measure the degree of target concentration and thus serve as a readout for sites of pain generation. Further, when PET is combined with the spatial and contrast resolution afforded by magnetic resonance imaging, nuclear medicine physicians and radiologists can potentially identify pain drivers with greater accuracy and confidence. Clinical PET imaging approaches with fluorine-18 fluorodeoxyglucose, fluorine-18 sodium fluoride, and sigma-1 receptor PET radioligand and translocator protein radioligands to isolate the source of pain are described here.
Total Hip Replacement: Exchange of a Loose Perforated Press-Fit Cup with a Porous-Coated Press-Fit Cup in a Dog
Authors: Colthurst Stephanie M.M. , Simcock James O. , Cashmore Ricky G. 
Published: September 2020

Abstract
Objective The aim of this study was to report the successful revision of a loose perforated press-fit cup by exchange with a porous-coated press-fit cup while maintaining the original locking stem and head–neck unit.
Methods Cup revision was performed in a dog with a loose acetabular cup that had undergone total hip replacement with a perforated press-fit cup and locking stem 29 months previously. The original locking stem was well integrated. A single session cup exchange was performed by implantation of a 28-mm porous-coated press-fit cup with 16 mm internal diameter to allow the original locking stem and head–neck unit to be preserved.
Results Revision total hip arthroplasty by cup exchange resulted in excellent functional outcome with long-term follow-up 18 months postoperatively. There were no intraoperative or postoperative complications.
Conclusion A loose perforated press-fit cup was successfully revised with a porous press-fit cup while allowing preservation of the initial locking stem and head–neck unit.
Change to Hearing Loss–Related Risks and Screening in Preterm Infants
Authors: Zhu Xiaodan , Lei Xiaoping , Dong Wenbin 
Published: September 2020

Hearing loss is one of the most common congenital defects in infancy; it increases speech and language delays and adversely affects academic achievement and socialemotional development. The risk of hearing loss in premature infants is higher than that in normal newborns, and because of the fragility of the auditory nervous system, it is more vulnerable to different risk factors. The hearing screening guidelines in current use were proposed by the American Academy of Pediatrics and updated in 2007, but there are no uniform guidelines for hearing screening in preterm infants. This review focuses on the risk factors related to hearing loss in premature infants, hearing screening strategies, and reasons for failure. The aim is to provide a more comprehensive understanding of hearing development in preterm infants to achieve early detection and early intervention. At the same time, attention should be paid to delayed auditory maturation in preterm infants to avoid excessive intervention.
Key Points