bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Differentially Expressed Genes and Processes in Myocardial Tissue of Liver-Specific Gck Knockout Mice
Authors: Li Hui , Xu Wei , Mao Yiqing , Wang Xi , Zhang Ruoxuan , Li Binghua , Bai Zhen , Irwin David M , Niu Gang , Tan Huanran 
Published: June 2020

Abstract
Background: Diabetic cardiomyopathy is a ventricular disease caused by diabetes mellitus. Abnormalities in the function of the glucokinase (GCK) play an important role in the development of diabetes. The present study is aimed at exploring changes in gene expression and related molecular mechanisms of diabetic myocardial injury in Gck knockout mice. Methods: Liver-specific glucokinase gene knockout mice( Gck w/- ) and wide type ( Gck w/w )mice generated using the Cre-loxP gene targeting strategy of 30- and 60-weeks of age were used in these studies. Determination of liver glucokinase enzyme activity, liver glycogen content and serum biochemistry parameters reflect the metabolic disorder in these mice. Echocardiography and surface electrocardiographs were used to evaluate cardiac function. Superoxide dismutase activity and malondialdehyde levels reflect oxidative stress in the myocardium. RNAseq, GO enrichment analysis and qPCR wereused to detect differences in the myocardial gene expression profiles of Gck w/- and Gck w/w mice. Results: Hyperglycemia and insulin resistance induced by decreased liver glucokinase expression and enzyme activity throughout the life of heterozygous Gck knockout mice do not yield body weight significant difference. However, prolonged PR interval and QRS duration, decreased left ventricular diameter and increased thickness of the posterior wall of the left ventricle were accompanied by increase of PAS and Masson positive substances in the myocardium of 60-week-old Gck knockout mice. RNAseq analysis showed that genes related tothe myosin heavy and light chains, insulin signaling pathway and oxidative phosphorylation were significantly differentially expressedbetween60-week-old Gck w/- and Gck w/w mice. Phosphorylation of AMPKβ1 and ACC in 60-week-old Gck knockout mice was decreased. Conclusions: Liver-specific Gck knock-out can induce myocardial fibrosis at an early stage and diabetic myocardial injury at alate stage. Through this process, the proportion of myosin heavy chain and light chain falls out of balance, the insulin signal pathway becomes down regulated and mitochondrial oxidative stress is up regulated, leading to myocardial disease.
Biodiversity of new lytic bacteriophages infecting Shigella spp. in fresh water environment
Authors: Shahin Khashayar , Barazandeh Mohadeseh , Hedayatkhah Abolghasem , Mansoorianfar Mojtaba , Wang Ran 
Published: June 2020

Abstract
Background

Bacteriophages, viruses that infects and replicates within prokaryotic cells are the most abundant life forms in the environment, yet the vast majority of them have not been properly reported or even discovered. Almost all reported bacteriophages infecting the Enterobacteriaceae family, with E. coli being the major subject of the study, have been isolated from wastewater, sewage, and effluent resources. In the present study we focused on the distribution and biodiversity of Shigella phages in an aquatic ecosystem.

Results

While no Shigella bacteria was recovered from the Yangtze River, three lytic phages were isolated from this ecosystem and were subjected to biological, morphological, and genomic characteristics. Comparative genomics and phylogenetic analyses demonstrated that vB _SflM_004 isolate belongs to Myoviridae family, Felixounavirus genus of Ounavirinae subfamily, vB_SdyM_006 was classified under the same family, however, it is suggested to be in a new genus under Tevenvirinae subfamily with some other related bacteriophages. vB_SsoS_008 phage belongs to the Siphoviridae family, Tunavirus genus, Tunavirinae subfamily. The phages did not harbor any genes involved in the lysogenic cycles, and showed a high temperature and pH stability.

Conclusions

It can be concluded that isolation of bacteriophages could be independent of their bacterial host presence in the isolation environment.
Corticosteroids in COVID-19; is it rational? A systematic review and meta-analysis
Authors: Budhathoki Pravash , Shrestha Dhan Bahadur , Rawal Era , Khadka Sitaram 
Published: June 2020

Abstract
Background

Due to a lack of definitive treatment, many drugs were repurposed for COVID-19 treatment, among them corticosteroid is one. However, its benefit or harm while treating COVID-19 is not fully studied. Thus, we conducted this meta-analysis to assess the rationality on the use of corticosteroids in COVID-19.

Methods

Pubmed, Medline, Clinicaltrials.gov, Cochrane library, and Preprint publisher were searched. In the qualitative synthesis, 41 and quantitative 40 studies were included using PRISMA guidelines. Assessment of heterogeneity was done using the I-squared (I 2 ) test and random/fixed-effect analysis was done to determine the odds/risk ratio.

Results

We found severely ill COVID-19 patients almost 5 (OR 4.78, 2.76-8.26) times higher odds of getting corticosteroids during their treatment. Similarly, the odds for corticosteroids in addition to standard of care (SOC) were approximately 4 (OR 4.09, 1.89-8.84) times higher among intensive care unit (ICU) patients than non-ICU ones. A higher mortality risk with corticosteroids receiving group compared with the SOC alone (RR 2.01, 1.12-3.63) was observed. Niether increased discharge rate (RR 0.79, 0.63-0.99) nor recovery/ improvement rate was shown among corticosteroids group (OR 0.24, 0.13-0.43). Approximately, the overall 4 days longer hospital stay was found among the treatment groups (MD 4.19, 2.57-5.81). For the negative conversion of reverse transcription- polymerase chain reaction (RT-PCR), approximately 3 days (MD 2.42, 1.31-3.53) delay was observed with corticosteroids treatment cases.

Conclusion

Our study concludes that more severe and critically ill patients tend to get corticosteroids and the mortality risk increases with the use of corticosteroids. With the use of corticosteroids, delayed recovery and a longer hospital stay were observed.
Determination of minimum number of random SNP for accurate population classification in rice (Oryza sativa L.)
Authors: Wang Shan , Ye Junhua , Xu Qun , Xu Xin , Yang Yingying , Zhang Mengchen , Feng Yue , Yuan Xiaoping , Yu Hanyong , Wang Yiping , Wei Xinghua , Yang Yaolong 
Published: June 2020

Abstract
Background: Classification of germplasm collections is of great importance for both the conservation and utilization of genetic resources. Thus, it is necessary to estimate and classify rice varieties in order to utilize these germplasms more efficiently for rice breeding. However, molecular classification of large germplasm collections can be costly and labor-intensive. Development of an informative panel of a few markers would allow for rapid and cost-effective assignment of crops to genetic sub-populations.Results: Here, the minimum number of random SNP for rice classification (MNRSRC) was studied using a panel of 51 rice varieties belonging to different sub-groups. Through the genetic structure analysis, the rice panel can be obviously divided into five subgroups. The estimation of the MNRSRC was performed using SNP random sampling method based on genetic diversity and population structure analysis. In the genetic diversity analysis, statistical analysis of the coefficient of variation (CV) was performed for MNRSRC estimation, and we found that CV variation tended to plateau when the number of SNP was around 200, which was verified by the both cross-validation error of K value and correlation analysis of genetic distance. When the number of SNPs was greater than 200, the distribution of cross-validation error value tended to be similar, and correlation coefficients, almost greater than 0.95, exhibited small range of variation. In addition, we found that MNRSRC might not be affected by the number of varieties and the type of varieties.Conclusion: The estimation of the MNRSRC was performed using SNP random sampling method based on genetic diversity and population structure analysis. The results demonstrated that at least about 200 random filtered SNP loci were required for classification in a rice panel. In addition, we also found that MNRSRC might not be affected by the number of varieties and the type of varieties. The study on MNRSRC in this study can provide a reference and theoretical basis for classification of different types of rice panels.
Hairball Buster: A Graph Triage Method for Viewing and Comparing Graphs
Authors: Allen Patrick , Matties Mark , Peterson Elisha 
Published: June 2020

Abstract
Hairball buster (HB) (also called node-neighbor centrality or NNC) is an approach to graph analytic triage that uses simple calculations and visualization to quickly understand and compare graphs. Rather than displaying highly interconnected graphs as ‘hairballs’ that are difficult to understand, HB provides a simple standard visual representation of a graph and its metrics, combining a monotonically decreasing curve of node metrics with indicators of each node’s neighbors’ metrics. The HB visual is canonical, in the sense that it provides a standard output for each node-link graph. It helps analysts quickly identify areas for further investigation, and also allows for easy comparison between graphs of different data sets. The calculations required for creating an HB display is order M plus N log N, where N is the number of nodes and M is the number of edges. This paper includes examples of the HB approach applied to four real-world data sets. It also compares HB to similar visual approaches such as degree histograms, adjacency matrices, blockmodeling, and force-based layout techniques. HB presents greater information density than other algorithms at lower or equal calculation cost, efficiently presenting information in a single display that is not available in any other single display.
Komparasi Waktu Algoritma RSA dengan RSA-CRT Base On Computer
Authors: Wibowo Nur Cayo Hendro , Umam Khotibul , Khaq Albadru Muh Izul , Rizki Favian Agung 
Published: June 2020

<p align="center"><em>Cryptography is a branch of mathematics. Asymmetric algorithms such as RSA, including the type of cryptography that is more widely used to be implemented in everyday life because it has an algorithm that is not easy to be misused by others. However, the encryption and decryption process of RSA requires quite a long time. For this reason, RSA - CRT emerged. In this study a time comparison between the RSA algorithm and the RSA-CRT Base On Computer is done. The purpose of this study was to determine the execution time of the RSA-CRT algorithm using PHP.</em></p><p><em>This research uses the Research and Development or R&amp;D method, with the R&amp;D method created by the product development RSA-CRT algorithm application from UIN Walisongo Semarang based on computer. Based on the data analysis, the conclusion is that although the manual calculation of the RSA-CRT algorithm takes longer than the others in this case RSA. But have a faster time when using PHP.</em></p><p align="center"> </p>
Assessing the benefits of improving the resilience of water distribution networks
Authors: Rulleau Bénédicte 
Published: June 2020

AbstractOver the last 15 years, particular attention has been paid to the protection and security of so-called critical infrastructures, including drinking water distribution networks (WDN). Infrastructure managers are seeking to ensure their security and improve their resilience. However, the question of the economic efficiency and of the economic benefits provided by such measures remains open. The purpose of this article is to contribute to this debate. It presents the results of a Choice Experiment survey aimed at estimating the benefits of measures to protect against a potential cyberattack in the territory of Eurométropole de Strasbourg in France. The aggregate benefits of two resilience programs are assessed. They help make ‘optimal’ and informed decisions from a cost–benefit perspective.
Protective Effect of Vernonia Cinerea Against Sunset Yellow Induced Anxiogenic Behaviour in Mice Model: Counteract Oxidative Damage based Approach
Authors: Krishnan Sadayan N. , Talari Divya , Rajangam Jayaraman , Pujari Latha , Palei Narahari N. , Balaji Anna , Surendran Vijayaraj 
Published: June 2020

Background:
Sunset yellow is a commonly used synthetic food additive in various food
beverages. Over usage of this colorant induces the anxiogenic behaviour in mice through oxidative
stress. Vernonia cinerea reverses anxiogenic behaviour by counteracting oxidative stress.

Objective:
The study was designed to screen the protective effect of Vernonia cinerea extract against
sunset yellow induced anxiogenic behaviour in mice through counteracting oxidative damage.

Methods:
The anxiogenic behaviour was induced by the administration of sunset yellow (20 mg/kg,
p.o) for 30 days. Ethanol extract of aerial parts of Vernonia cinerea (EEVC) (400 mg/kg, p.o) was
administered after 1 hour of sunset yellow treatment. Diazepam (1 mg/kg, p.o) was used as a reference
drug for comparisons.

Results:
FT-IR studies of the extract were done by comparing with standards to determine the presence
of phenols and flavonoids. Vernonia cinerea significantly improved the altered anxiogenic behaviour
of sunset yellow treated animals. Significant increase in the brain antioxidant enzymes catalase
(CAT), superoxide dismutase (SOD) and neurotransmitter Gamma-aminobutyric acid (GABA)
level and decrease in the lipid peroxidation and glutamate neurotransmitter were observed in
Vernonia cinerea treated group and also this extract reversed the hippocampus CA3 region abnormalities
such as perivascular oedema, haemorrhage and severe vocalisation in sunset yellow group.

Conclusion:
The Vernonia cinerea possesses significant antioxidant, antiradical and GABA enhancing effects against
sunset yellow induced anxiogenic animals.
Effectiveness of a Voice-Based Mental Health Evaluation System for Mobile Devices: Prospective Study
Authors: Higuchi Masakazu , Nakamura Mitsuteru , Shinohara Shuji , Omiya Yasuhiro , Takano Takeshi , Mitsuyoshi Shunji , Tokuno Shinichi 
Published: June 2020

BackgroundWe developed a system for monitoring mental health using voice data from daily phone calls, termed Mind Monitoring System (MIMOSYS), by implementing a method for estimating mental health status from voice data.ObjectiveThe objective of this study was to evaluate the potential of this system for detecting depressive states and monitoring stress-induced mental changes.MethodsWe opened our system to the public in the form of a prospective study in which data were collected over 2 years from a large, unspecified sample of users. We used these data to analyze the relationships between the rate of continued use, the men-to-women ratio, and existing psychological tests for this system over the study duration. Moreover, we analyzed changes in mental data over time under stress from particular life events.ResultsThe system had a high rate of continued use. Voice indicators showed that women have more depressive tendencies than men, matching the rate of depression in Japan. The system’s voice indicators and the scores on classical psychological tests were correlated. We confirmed deteriorating mental health for users in areas affected by major earthquakes in Japan around the time of the earthquakes.ConclusionsThe results suggest that although this system is insufficient for detecting depression, it may be effective for monitoring changes in mental health due to stress. The greatest feature of our system is mental health monitoring, which is most effectively accomplished by performing long-term time-series analysis of the acquired data considering the user’s life events. Such a system can improve the implementation of patient interventions by evaluating objective data along with life events.
Mortality and Recovery Associated with Kidney Failure due to Acute Kidney Injury
Authors: Shah Silvi , Leonard Anthony C. , Harrison Kathleen , Meganathan Karthikeyan , Christianson Annette L. , Thakar Charuhas V. 
Published: June 2020

Background and objectives
AKI requiring dialysis is a contributor to the growing burden of kidney failure, yet little is known about the frequency and patterns of recovery of AKI and its effect on outcomes in patients on incident dialysis.

Design, setting, participants, & measurements
Using the US Renal Data System, we evaluated a cohort of 1,045,540 patients on incident dialysis from January 1, 2005 to December 31, 2014, retrospectively. We examined the association of kidney failure due to AKI with the outcome of all-cause mortality and the associations of sex and race with kidney recovery.

Results
Mean age was 63±15 years, and 32,598 (3%) patients on incident dialysis had kidney failure due to AKI. Compared with kidney failure due to diabetes mellitus, kidney failure attributed to AKI was associated with a higher mortality in the first 0–3 months following dialysis initiation (adjusted hazard ratio, 1.28; 95% confidence interval, 1.24 to 1.32) and 3–6 months (adjusted hazard ratio, 1.16; 95% confidence interval, 1.11 to 1.20). Of the patients with kidney failure due to AKI, 11,498 (35%) eventually recovered their kidney function, 95% of those within 12 months. Women had a lower likelihood of kidney recovery than men (adjusted hazard ratio, 0.86; 95% confidence interval, 0.83 to 0.90). Compared with whites, blacks (adjusted hazard ratio, 0.68; 95% confidence interval, 0.64 to 0.72), Asians (adjusted hazard ratio, 0.82; 95% confidence interval, 0.69 to 0.96), Hispanics (adjusted hazard ratio, 0.82; 95% confidence interval, 0.76 to 0.89), and Native Americans (adjusted hazard ratio, 0.72; 95% confidence interval, 0.54 to 0.95) had lower likelihoods of kidney recovery.

Conclusions
Kidney failure due to AKI confers a higher risk of mortality in the first 6 months compared with kidney failure due to diabetes or other causes. Recovery within 12 months is common, although less so among women than men and among black, Asian, Hispanic, and Native American patients than white patients.
BLENDED LEARNING APPROACH USING FLIPPED CLASSROOM MODEL ON KAIWA III
Authors: Yuniarsih Yuniarsih , Hapsari Eky Kusuma , Zakaria Muhamad 
Published: June 2020

<p><em>This study aims to determine the effectiveness of using blended learning flipped classroom model on learning outcomes for Japanese conversation. The method used in this study is pre-experimental, with one group pre-test post-test design. The population in this study were students of the Japanese Language Education Study Program, Universitas Negeri Jakarta, 2019/2020 academic year with a sample of 22 students of Kaiwa III. The instrument used were tests, in the form of a pretest-posttest, and questionnaire. From the statistical data calculation based on the results of the pretest and the posttest show that the t<sub>table</sub> value at the 5% significance level is 2.018, while the t<sub>calculate</sub> value in this study is 4.08. Since the t<sub>calculate</sub> is higher that the t<sub>table</sub> , it shows that the hypothesis alternative (Ha) of this study is accepted, which proved the using of flipped classroom model on learning outcomes for Japanese conversation is effective.</em></p>
NOTAS SOBRE LA FACULTAD DE AMPLIAR O RECTIFICAR LA DEMANDA UNA VEZ INICIADO EL JUICIO ORDINARIO
Authors: Alcalde Jaime 
Published: June 2020

This paper tries to clarify the meaning and scope regarding the power to expand and rectify the civil complaint enlargement that Article 261 CPC grants to the plaintiff once the ordinary trial has begun. It differs from the possibility granted by Article 312 CC, which states that admitted that both parties two parties, in their reply or rejoinder briefs, may extend, add or modify the actions and exceptions that they have formulated in their demand or response, with the condition that this alteration does not affect the main controversy raised before the court. With the support of the historical origin of this rule and a systematic analysis considering the context of the Code of Civil Procedure, I will try to prove that the extension or rectification of the demand only includes the written account presented to the court and not when there was already a behavior of the defendant who assumes the material terms of the judicial controversy and locks the procedural relationship. Furthermore, this possibility does not extend to the reformed procedures, although it is included in the new Civil Procedure Code with some changes in its formulation
Redesigning Business Process to Comply with the New Revenue Recognition Standard Using Process Mining
Authors: Wang Yunsen , Chiu Tiffany , Chiu Victoria 
Published: June 2020

ABSTRACT
This study proposes a framework and develops a prototype that redesigns revenue cycle business processes to comply with the new revenue recognition standard Revenue from Contracts with Customers (ASU No. 2014-09 FASB 2014). The new standard aims to report the nature, amount, timing, and uncertainty of revenue; however, the accounting literature and profession have raised concerns regarding companies' compliance with the new standard, and urged companies to assess their practices and modify their accounting information systems accordingly. This study utilizes process mining to redesign a revenue cycle business process framework to consider single/multiple performance obligation(s), transaction price allocation, sales discounts granted/forfeited, and sales returns scenarios. In addition, a prototype is developed to detect non-standard sales transactions automatically and identify potential violations of the new standard. Findings in this study contribute to emerging technologies in accounting, internal auditing, and revenue recognition research, and are expected to be of interest to academics and practitioners.