bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alternative polyadenylation is a determinant of oncogenic Ras function
Authors: Subramanian Aishwarya , Hall Mathew , Hou Huayun , Mufteev Marat , Yu Bin , Yuki Kyoko E. , Nishimura Haruka , Sathaseevan Anson , Lant Benjamin , Zhai Beibei , Ellis James , Wilson Michael D. , Daugaard Mads , Derry W. Brent 
Published: June 2020

ABSTRACTAlternative polyadenylation of pre-mRNA has been recently shown to play important roles in development and cancer. Activating mutations in the Ras oncogene are common drivers of many human cancers but the mechanisms by which they cooperate with alternative polyadenylation are not known. By exploiting the genetics ofC. elegans, we identifiedcfim-1/CFIm25, a subunit of the alternative polyadenylation machine, as a key determinant of hyperactive Ras function. Ablation ofcfim-1increased penetrance of multivulva phenotype inlet-60/Rasgain-of-function (gf) mutant through shortening of transcripts at the 3’ untranslated region, including p21 activated kinasepak-1/PAK1and multidrug transportermrp-5/ABCC1. Depletion of CFIm25 in human KRAS-driven cancer cells resulted in a similar shortening of 3’ untranslated regions in thePAK1andABCC1transcripts, which caused an epithelial-to-mesenchymal transition and increased cell migration. Exploiting the mechanisms by which alternative polyadenylation affects activated oncogene output could offer novel approaches for the treatment of Ras-driven tumors.
Calculating the optimal hematocrit under the constraint of constant cardiac power
Authors: Sitina M. , Stark H. , Schuster S. 
Published: June 2020

AbstractIn humans and higher animals, a trade-off between sufficiently high erythrocyte concentrations to bind oxygen and sufficiently low blood viscosity to allow rapid blood flow has been achieved during evolution. Optimal hematocrit theory has been successful in predicting hematocrit values of about 0.3 - 0.5, in very good agreement with the normal values observed for humans and many animal species. However, according to those calculations, the optimal value should be independent of the mechanical load of the body. This is in contradiction to the exertional increase in hematocrit observed in some animals called natural blood dopers and to the illegal practice of blood boosting in high-performance sports. In this study, we calculate the optimal hematocrit under two different constraints - under a constant driving pressure and under constant cardiac power – and show that the optimal hematocrit under constant cardiac power is higher than the normal value, ranging from 0.5 to 0.7. We use this result to explain the tendency to better exertional performance at an increased hematocrit.Statement of SignificanceIn humans and higher animals, erythrocytes comprise a volume fraction (hematocrit) of 30-50 % of the blood. Mathematical calculations based on the assumption of constant blood pressure show that the optimal hematocrit value is indeed in that range. However, this optimum should apply to both rest and physical exertion, which is in contradiction to the increase in hematocrit observed in some animals called natural blood dopers and to the illegal practice of blood boosting in sports. Here, we calculate the optimal value based on the alternative constraint of constant cardiac power. We show that this results in a higher optimal value, ranging from 50 to 70 %. In this way, we explain the better exertional performance at an increased hematocrit.
Insight into vaccine development for Alpha-coronaviruses based on structural and immunological analyses of spike proteins
Authors: Shi Yuejun , Shi Jiale , Sun Limeng , Tan Yubei , Wang Gang , Guo Fenglin , Hu Guangli , Fu Yanan , Fu Zhen F. , Xiao Shaobo , Peng Guiqing 
Published: June 2020

AbstractCoronaviruses that infect humans belong to theAlpha-coronavirus(including HCoV-229E) andBeta-coronavirus(including SARS-CoV and SARS-CoV-2) genera. In particular, SARS-CoV-2 is currently a major threat to public health worldwide. However, no commercial vaccines against the coronaviruses that can infect humans are available. The spike (S) homotrimers bind to their receptors through the receptor-binding domain (RBD), which is believed to be a major target to block viral entry. In this study, we selectedAlpha-coronavirus(HCoV-229E) andBeta-coronavirus(SARS-CoV and SARS-CoV-2) as models. Their RBDs were observed to adopt two different conformational states (lying or standing). Then, structural and immunological analyses were used to explore differences in the immune response with RBDs among these coronaviruses. Our results showed that more RBD-specific antibodies were induced by the S trimer with the RBD in the “standing” state (SARS-CoV and SARS-CoV-2) than the S trimer with the RBD in the “lying” state (HCoV-229E), and the affinity between the RBD-specific antibodies and S trimer was also higher in the SARS-CoV and SARS-CoV-2. In addition, we found that the ability of the HCoV-229E RBD to induce neutralizing antibodies was much lower and the intact and stable S1 subunit was essential for producing efficient neutralizing antibodies against HCoV-229E. Importantly, our results reveal different vaccine strategies for coronaviruses, and S-trimer is better than RBD as a target for vaccine development inAlpha-coronavirus. Our findings will provide important implications for future development of coronavirus vaccines.ImportanceOutbreak of coronaviruses, especially SARS-CoV-2, poses a serious threat to global public health. Development of vaccines to prevent the coronaviruses that can infect humans has always been a top priority. Coronavirus spike (S) protein is considered as a major target for vaccine development. Currently, structural studies have shown thatAlpha-coronavirus(HCoV-229E) andBeta-coronavirus(SARS-CoV and SARS-CoV-2) RBDs are in lying and standing state, respectively. Here, we tested the ability of S-trimer and RBD to induce neutralizing antibodies among these coronaviruses. Our results showed that Beta-CoVs RBDs are in a standing state, and their S proteins can induce more neutralizing antibodies targeting RBD. However, HCoV-229E RBD is in a lying state, and its S protein induces a low level of neutralizing antibody targeting RBD. Our results indicate thatAlpha-coronavirusis more conducive to escape host immune recognition, and also provide novel ideas for the development of vaccines targeting S protein.
The peer effect of training on farmers' pesticides application: a spatial econometric approach
Authors: Zhou Li , Zhang Fan , Zhou Shudong , Turvey Calum G. 
Published: June 2020

PurposeThe purpose of this paper is to examine the relationships of technical training and the peer effects of technical training with farmers' pesticide use behaviors.Design/methodology/approachThis study uses survey data from 300 peanut growers in Zoucheng County, Shandong, China, in 2016 and employs spatial econometric models to examine the relationships of technical training and the peer effects of technical training with farmers' pesticide use behaviors.FindingsThis paper reveals that important peer effects can be channeled through technical training and that these peer effects are sufficiently significant to encourage neighboring farmers to reduce the amount of pesticide use, to transform the structure of pesticide use, and to increase the usage amount of low-toxicity, low-residue pesticide use per hectare. The estimated parameters for the peer effects from technical training are significantly larger than those from technical training alone, which suggests that the technical training of neighboring farmers plays a greater role than technical training for farmers individually.Originality/valueThe research finds that technical training within smaller, localized, groups can induce previously unobservable spillover effects, and this provides a scientific, theoretical and empirical justification for agricultural technology extension that can lead to a rapid, effective transformation of applying new agricultural technologies in an environmentally sensitive and economically sustainable manner.
Influencing factors of construction professionals’ burnout in China: a sequential mixed-method approach
Authors: Sun Mouxuan , Zhu Fangwei , Sun Xiuxia 
Published: June 2020

PurposeThe present study investigated how different factors interact and work in concert to influence construction professionals’ burnout (hereafter CPs’ burnout) in China.Design/methodology/approachA sequential mixed-method approach was chosen for this research. Twenty-two interviews were conducted and analysed, and we identified ten influencing factors associated with CPs’ burnout. Subsequently, a sample of 232 questionnaires was analysed using fuzzy-set qualitative comparative analysis (fsQCA) to ascertain the eight configurations of CPs’ high and low burnout.FindingsThe key findings include the following: first, perceived workload, role ambiguity, role conflict, emotional demand, work-home interference, relationships with supervisors, autonomy, fairness of rewards, support from project team and self-efficacy are the ten factors influencing CPs’ burnout; second, experienced and less experienced construction professionals take different paths towards high or low burnout; and third, among construction professionals, perceived workload and burnout are not necessarily correlated. We found that autonomy plays a crucial role in this process.Originality/valueThis is one of the first studies to adopt a configurational approach for understanding influencing factors of CPs’ burnout. The strength of the present study is its sequential mixed-method approach, which forms a loop between the qualitative and quantitative studies.
Researching on the edge: emancipatory praxis for social justice
Authors: Hutton Martina , Heath Teresa 
Published: June 2020

Purpose
This paper aims to provoke a conversation in marketing scholarship about the overlooked political nature of doing research, particularly for those who research issues of social (in)justice. It suggests a paradigmatic shift in how researchers might view and operationalise social justice work in marketing. Emancipatory praxis framework offers scholars an alternative way to think about the methodology, design and politics of researching issues of social relevance.

Design/methodology/approach
This is a conceptual paper drawing on critical theory to argue for a new methodological shift towards emancipatory praxis.

Findings
As social justice research involves a dialectical relationship between crises and critique, the concept of emancipation acts as a methodological catalyst for furthering debate about social (in)justice in marketing. This paper identifies a set of methodological troubles and challenges that may disrupt the boundaries of knowledge-making. A set of methodological responses to these issues illustrating how emancipatory research facilitates social action is outlined.

Research limitations/implications
Emancipatory praxis offers marketing scholars an alternative methodological direction in the hope that more impactful and useful ways of knowing can emerge.

Practical implications
The paper is intended to change the ways that researchers work in practical and concrete terms on issues of social (in)justice.

Social implications
Although this paper is theoretical, it argues for an alternative methodological approach to research that reorients researchers towards a politicised praxis with emancipatory relevancy.

Originality/value
Emancipatory praxis offers a new openly politicised methodological alternative for addressing problems of social relevance in marketing. As a continuous political and emancipatory task for researchers, social justice research involves empirical encounters with politics, advocacy and democratic participation, where equality is the methodological starting point for research design and decisions as much as it is the end goal.
Construct creation from research questions
Authors: Forbes Sarah , Avis Mark 
Published: June 2020

PurposeConstruct Creation (CC) is a methodological problem occurring when a research process, instead of measuring an extant construct in the participant’s mind, creates the construct. The purpose of this paper is to argue that CC derives from problems around ecologically invalid research and attitudinal responses developed on the spot, both resulting from self-generated validity.Design/methodology/approachA between-subjects design was used to explore whether the personification prime (PP), a component of brand personality (BP) methodology, influenced the CC of BP for rocks. Analysis of qualitative data on how participants made their BP ratings in the absence of a PP was also completed.FindingsFindings revealed that a methodology can enable CC in the participant’s mind, despite the construct being ecologically invalid prior to them participating in the study. Analysis also revealed that participants will use varied, and sometimes elaborate, strategies to enable CC and provide researchers with the answers to their questions.Research limitations/implicationsPrevious research has drawn attention to CC as a problem but the implications of prior research have so far been “sidestepped”. Consequently, this paper demonstrates CC and why it is a problem, while rebutting some arguments made in prior research for sidestepping CC.Practical implicationsCC is a potentially serious methodological problem that can result in invalid findings informing or misdirecting theory used by practitioners. As such, this paper proposes methods to ameliorate CC and improve ecological validity of future research.Originality/valueThis study will contribute to methodological literature by refocusing attention to the currently neglected problem of CC and by proposing a model of CC by participants.
A methodology for applying the shop floor management method for sustaining lean manufacturing tools and philosophies: a study of an automotive company in Brazil
Authors: Gaspar Flávio , Leal Fabiano 
Published: June 2020

Purpose
The purpose of this paper is to define a method for sustain lean tools and philosophies on a manufacturing environment.

Design/methodology/approach
An in-depth action research (AR) methodology procedures applied in two cycles were conducted in an automobile company located in southeastern Brazil. The objective is to test the applicability of the shop floor management (SFM) implementation model as presented by Hanenkamp (2013).

Findings
The SFM model presented by Hanenkamp (2013) has been outlined, detailed and applied in practice. Opportunities for improvement during the application process of this model were verified by changes in its steps. After developing the AR, the authors have shown that the SFM model, as proposed in this paper, can indeed assist managers in applying and maintaining lean manufacturing practices on the shop floor.

Originality/value
This paper contributes by aiding in filling the gap between practical applications and the sustainability of lean manufacturing concepts and solutions. Furthermore, the guidelines introduced serve as a benchmark for other companies interested in the topic.
Managing the professional and qualification and innovative potential of youth in the system of higher education
Authors: Ivanchenko Olga S. , Tikhonovskova Svetlana A. , Zalevskaya Anna A. , Belasheva Irina V. 
Published: June 2020

PurposeThe purpose of the paper is to study the factors that influence the effectiveness of the professional and qualification and innovative potential of youth in a university environment. The authors focus on the main institutional factors of the external and internal character, which are the barriers to this reproduction process and do not bring the expected result—renovation of the qualitative structure of the workforce. The authors determine the essence and main characteristics of the process of reproduction of innovative and professional and qualification potential in higher school by the example of Russia.Design/methodology/approachMethodology of the work includes SWOT analysis, which allows distinguishing the potential (D) of strong (F) and weak (G) opportunities and the level of indicators of realization of (R) opportunities (V) and threats (U) of the research object.FindingsIt is substantiated that factors-barriers of innovative and professional and qualification potential could be overcome with the help of the formation of the mechanism of interaction of labor market, market of educational services, and innovative university complexes.Practical implicationsThe obtained results allow determining the main threats with a high probability of implementation in the process of formation of professional and qualification potential in the system of higher education and forming the perspectives of solving this problem.Originality/valueOriginality of this study consists in the totality of obtained results, which show the specific features of reproduction of innovative and professional and qualification potential of youth: determination of strengths and weaknesses, opportunities and threats of the process of formation of innovative and professional and qualification potential in the system of higher education.
How does a destination’s food image serve the common targets of the UNESCO creative cities network?
Authors: Yılmaz Gökhan , Kılıçarslan Doğuş , Caber Meltem 
Published: June 2020

PurposeAs one of the United Nations Educational, Scientific and Cultural Organization initiatives, the creative cities network (CCN) declares the cities that are creative in the contexts of music, gastronomy, design, etc., with the aim of promoting cooperation amongst the member cities and maintaining sustainable urban development. This study aims to identify the destination food image of Gaziantep in Turkey, which is a member gastronomy city of the CCN since 2015. Identified destination food image elements were connected to the common targets of the CCN to show how the city may contribute to the network objectives.Design/methodology/approachA two-stage research process was used in the study. First, qualitative approach was adopted for the clarification of projected and perceived destination food image elements. Projected image elements were derived from a content analysis performed on a totally 113 official, semi-official and unofficial online documents in Turkish and English. Perceived destination food image elements were identified by face-to-face interviews, conducted on 10 participants. As a result, 18 projected and 20 perceived destination food image elements were obtained. These were then grouped under 4 main and 22 sub-categories. At the second stage, destination food image elements were matched with common targets of the CCN.FindingsDestination food image elements, obtained by two qualitative studies, are grouped under 4 main and 22 sub-categories as follows: gastronomic identity (with sub-categories of destination’s identity and local culinary culture); diversity of the destination (with sub-categories of attractiveness of the local food, ease of promotion and high brand value); gastronomic attractions (with sub-categories of restaurants and cafes, culinary museums, farmer markets, orchards, gastronomy tours, gastronomy events (e.g. festivals, competitions), culinary education, books on gastronomy, certification systems, organizations, street foods and vendors and handmade or homemade foods); and qualified workforce and stakeholders (with sub-categories of expert chefs and cooks, specialist suppliers, service personnel, locals and local authorities). These are then connected to the common CCN targets (e.g. cuisine, tourism and festivals; extension of the creative value chain; fostering cultural creativity; and sustainability).Originality/valueThis is one of the early research attempts in examining a member gastronomy city’s food image elements and the role that they played in the success of the CCN’s common targets. Moreover, the study contributes to the literature on the identification of (projected and perceived) destination food image by using content analysis.
Playing in the backstore: interface gamification increases warehousing workforce engagement
Authors: Passalacqua Mario , Léger Pierre-Majorique , Nacke Lennart E. , Fredette Marc , Labonté-Lemoyne Élise , Lin Xinli , Caprioli Tony , Sénécal Sylvain 
Published: June 2020

PurposeIn a warehouse setting, where hourly workers performing manual tasks account for more than half of total warehouse expenditure, a lack of employee engagement has been directly linked to company performance. In this article, the authors present a laboratory experiment in which two gamification elements, goal setting and feedback, are implemented in a wearable warehouse management system (WMS) interface to examine their effect on user engagement and performance in an item picking task. Both implicit (neurophysiological) and explicit (self-reported) measures of engagement are used, allowing for a richer understanding of the user's perceived and physiological state.Design/methodology/approachThis experiment uses a within-subject design. Two experimental factors, goals and feedback, are manipulated, leading to three conditions: no gamification condition, self-set goals and feedback and assigned goals and feedback. Twenty-one subjects participated (mean age = 24.2, SD = 2.2).FindingsThis article demonstrates that gamification can successfully increase employee engagement, at least in the short-term. The integration of self-set goals and feedback game elements has the greatest potential to generate long-term intrinsic motivation and meaningful engagement, leading to greater employee engagement and performance.Originality/valueThis article explores the underlying effects of gamification through two of the most prominent motivational theories (self-determination theory [SDT] and goal-setting theory) and one of the leading employee engagement models (job demands-resource model [JD-R[ model). This provides a theory-rich interpretation of the data, which allows to uncover the motivational pathways by which gamification affects engagement and performance.
How to improve new product performance through customer relationship management and product development management: evidence from China
Authors: Yan Jianhui , Zheng Yu , Bao Jiaxin , Lu Chongyu , Jiang Yanhui , Yang Zhi , Feng Chulan 
Published: June 2020

PurposeThis paper aims to investigate how to improve new product performance in turbulent circumstances of emerging economies.Design/methodology/approachThis paper used regression analysis to examine the performance impact of customer relationship management (CRM) and product development management (PDM) concentration strategy in new product development (NPD). A detailed contingent analysis of the market and institutional environments in emerging economies is also conducted based on a survey of 114 Chinese high-tech manufacturers.FindingsThe research findings show that PDM has a stronger positive effect on new product performance than CRM in emerging economies and that the contingent effects of the market and institutional environment vary. More specifically, technological turbulence and enforcement inefficiency can positively moderate the relationship between CRM and new product performance, whereas the moderating effect of market turbulence on CRM is negative. Meanwhile, enforcement inefficiency negatively moderates the effect of PDM on new product performance, while the moderating effect of market turbulence on PDM is positive.Research limitations/implicationsThis paper is limited to a survey of high-tech manufacturing enterprises in China. Further research should continues to explore and document the strategic issue about NPD in emerging economies by longitudinal study.Originality/valueThis paper contributed to theoretical and practical initiatives on the strategic issue of NPD and provided firms a further understanding of how to select the right NPD strategy in emerging economies to improve new product performance.