bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penerapan Model PjBL Berbantuan Media Ular Tangga untuk Meningkatkan Kreativitas Peserta Didik Sekolah Dasar
Authors: Rodhiyah Afifah Rochmatur , Sakdiyah Siti Halimatus , Zuhro Luluk Faridatuz 
Published: December 2024

Abstract: Based on the diagnostic assessment, the results obtained from 28 children were 5 children in the very good category and 4 children in the good category with a level of creativity. The purpose of this research is to describe the use of the PjBL based snakes and ladders to increase the creativity of students in SBdP Class I SDN Bandungrejosari 1. This type of research was classroom action research conducted in two cycles. The stages of each cycle are planning, action, observation and reflection. The research subjects were class IB students at SDN Bandungrejosari 1. The data is collected by observation, documentation, and products analysis. Data analysis use qualitative and quantitative analysis. This research is said successfully if the percentage of creativity reaches 80 percent and the application of the PjBL reaches 90 percent. The results showed an increase in each indicator of creativity. The fluency indicator increased by 7.14 percent from the first cycle of 81.25 percent to 88.39 percent in the second cycle. The novelty indicator increased by 5.35 percent from the first cycle of 80.36 percent to 85.71 percent in the second cycle. The flexibility indicator increased by 2.68 percent from cycle I of 75.89 percent to 78.57 percent in cycle II, so it can be concluded that the PjBL based snakes and ladders can increase the creativity of students in Sbdp Class I SDN Bandungrejosari 1.
Abstrak: Berdasarkan asesmen diagnostik, didapatkan hasil dari 28 anak terdapat 5 anak dalam kategori sangat baik dan 4 anak dalam kategori baik tingkat kreativitasnya. Tujuan penelitian adalah mendeskripsikan penerapan model PjBL berbantuan media ular tangga untuk meningkatkan kreativitas peserta didik pada mata pelajaran SBdP Kelas I SDN Bandungrejosari 1. Jenis penelitian ini adalah penelitian tindakan kelas yang dilakukan dalam 2 siklus. Tahapan tiap siklus yakni perencanaan, tindakan, pengamatan dan refleksi. Subjek penelitian adalah peserta didik kelas IB SDN Bandungrejosari 1. Teknik pengumpulan data melalui observasi, dokumentasi, dan produk. Analisis data dilakukan dengan analisis kualitatif dan kuantitatif. Penelitian ini dikatakan berhasil apabila persentase kreativitas mencapai 80 persen dan penarapan model PjBL mencapai 90 persen. Hasil penelitian menunjukkan adanya peningkatan setiap indikator kreativitas. Indikator kefasihan meningkat sebesar 7, 14 persen, pada siklus I 81,25 persen sedangkan pada siklus II 88,39 persen. Indikator kebaruan meningkat sebesar 5,35 persen, pada siklus I 80,36 persen sedangkan pada siklus II 85,71 persen. Indikator fleksibilitas meningkat sebesar 2,68 persen, pada siklus I 75,89 persen sedangkan pada siklus II 78,57 persen, sehingga dapat disimpulkan bahwa model PjBL berbantuan media ular tangga dapat meningkatkan kreativitas peserta didik pada mata pelajaran Sbdp Kelas I SDN Bandungrejosari 1.
Cariotipo de<i> Incilius valliceps </i>(Anura: Bufonidae) y hallazgo de cromosomas sexuales (ZW/ZZ) revelados en macho fenotípico
Authors: Hernández-Guzmán Javier , Arias-Rodriguez Lenin 
Published: December 2024

The coastal toad Incilius valliceps, which have been little studied and understood from several biological and ecological perspectives. The aim of the present study was to describe the typical karyotype in mitosis of I. valliceps from two females and five adult males from two localities of Tabasco state, Mexico. All specimens were subjected to standard cytogenetic treatment. Assembly and karyotype interpretation were based on standard nomenclature. The typical karyotype in mitosis of I. valliceps was integrated by of 2n = 22 chromosomes. However, the presence of two types of karyotypes was observed, one characterized by homogametic males (ZZ) with a chromosome formula of 12msm+8T+2A (NF = 34) and a phenotypic male from La Lima with chromosome formula of genetic female (ZW) with 12msm+8T+1stt+1A (NF = 36). The results indicate sexual setback from the genetic female to a phenotypic male.
HUBUNGAN ASUPAN MAKAN, PERSEPSI CITRA TUBUH, DAN PERILAKU PENGENDALIAN BERAT BADAN DENGAN STATUS GIZI GURU DI PERGURUAN MUHAMMADIYAH RAWAMANGUN
Authors: Azizah Fina Nailatul , Rahayu Leni Sri 
Published: December 2024

Overnutrition is one of the most common nutritional problems in the adult age group. Overnutrition among working adults can harm productivity at work. This is because excess nutritional status can cause various non-communicable diseases such as type 2 diabetes mellitus, coronary heart disease, and cancer. The purpose of this study was to determine the relationship between food intake, body image perception, and weight control behavior with nutritional status at the Muhammadiyah College of Rawamangun. The research was conducted in August 2023, involving 61 teachers. The research method used is quantitative research with a cross-sectional design and total sampling method for sample selection. Data was analyzed using the Chi-Square statistical test. Dietary intake was measured using a 2x24-hour recall method, body image perception and weight control behavior were assessed using Body Shape Questionnaire (BSQ) Short Version also waist circumference, body weight and height were measured using anthropometric measurements. The results of data analysis indicate a significant relationship between energy intake, protein intake, fat intake, carbohydrate intake, body image perception, and weight control behavior with nutritional status based on BMI and waist circumference (P<0.05). However, no significant relationship was found between body image perception and weight control behavior with nutritional status based on waist circumference (p>0.05). In conclusion, there were significant relationship between energy intake, protein intake, fat intake, carbohydrate intake, body image perception, and weight control behavior with nutritional status based on BMI and waist circumference.
Determinants of Nutritional Status among Malnourished Children in Agricultural Areas
Authors: Susanto Tantut , Herminiati Ainia , Rohmawati Ninna , Yunanto Rismawan Adi , Rahmawati Ira , Bachtiar Syahroni , Merina Nuning Dwi , Zolkefli Yusrita Binti 
Published: December 2024

Stunting in children is a major global issue, especially in rural agricultural areas of Indonesia. Despite ongoing efforts to eliminate stunting, its prevalence remains high, adversely affecting children's cognitive and physical development. This study aimed to determine the factors influencing the nutritional status of stunted children in Indonesia’s agricultural districts. A cross-sectional analytical study was conducted in Jember District, Indonesia, involving 236 malnourished children aged 0 to 59 months. Using stratified random sampling, structured questionnaires were utilized to collect data on family functioning, child feeding attitudes, and practices. The results are Family Function (B=0.038; 95% CI= 0,535 – 1,658, p value= 0.047); Child Feeding Attitude (B=0.030; 95% CI= 0,882 – 1,681, p value= 0.023); and Child Feeding Practice (B=0.120; 95% CI= 0,686 – 1,971, p value= 0.000). There were significant correlations between family function (p value= 0.047), child-feeding attitudes (p value= 0.023), and child-feeding practice (p value= 0.000) with children's nutritional status. This study highlighted a significant relationship among family function, child-feeding attitude, and practices concerning the nutritional status of children experiencing stunting. It emphasizes the importance of improving family function and supporting healthy child-feeding practices. Targeted interventions based on local cultural understanding are critical for effectively reducing stunting.
Development of a high-throughput assay for monitoring adipogenesis in vitro
Authors: Giles Rachel , Durandt Chrisna , Ambele Melvin , Pepper Michael 
Published: December 2024

Abstract

The obesity pandemic is listed as a chronic disease by the World Health Organization, and is underpinned by an increase in fat cell formation (adipogenesis). This study aims to develop high-throughput screening assays (flow cytometry, spectrophotometry and RT-qPCR) for cost-effective and rapid monitoring of adipogenesis using a human adipose-derived stem cell differentiation model. Adipogenesis was successfully upscaled from 6- to 96-well plates. We found that the most efficient well size differs between the assays investigated. Adipogenic differentiation could best be assessed in a 48-well plate (flow cytometry and spectrophotometry), while a 12-well plate was more efficient for obtaining reliable RT-qPCR results. The cost associated with hypothetically screening 100 compounds (in a high-throughput setting) using the method with the lowest degree of variability and the highest degree of cost-effectiveness was spectrophotometry (48-well plate), followed by flow cytometry (48-well plate) and RT-qPCR (12-well plate). This study provides valuable information for designing and selecting the most efficient high-throughput adipogenesis assays to screen for potential drug candidates in the continuous search of novel and more effective pharmacological agents to combatting obesity and its related comorbidities.
Global, Regional, and National Burden of Benign Prostatic Hyperplasia: A Comprehensive Analysis from 1990 to 2021
Authors: Chen Xin , Yang Siyuan , He Zhangxin , Chen Zihao , Tang Xinling , Lin Yuxin , Huang Yuhua , Hou Jianquan , Wei Xuedong 
Published: December 2024

Abstract

Benign prostatic hyperplasia (BPH) is a common urological condition affecting elderly men worldwide. In 2021, there were 112,502 (95% UI: 88,131.8–142,634.2) thousand prevalent cases globally, compared to 50,705.8 (95% UI: 38,735.5–65,693.4) thousand cases in 1990, representing a 122% increase. Over the past 30 years, the burden of BPH in low and low-middle socio-demographic index (SDI) regions have shown an upward trend and is projected to continue increasing over the next 15 years. Middle-SDI regions are facing the heaviest absolute burden of BPH. Despite declining prevalence, incidence, and disability-adjusted life years rates in high-middle SDI regions, the absolute BPH burden remains high, ranking second among the five SDI regions. In contrast, high-SDI regions exhibit a relatively low and stable BPH burden, though significant variations exist even among countries within the high-SDI category. Additionally, the global 65–69 age group bears the highest burden, with the 40–44 and 80 + age groups showing increasing trends. The burden of BPH varies significantly across regions, socioeconomic statuses, and countries, yet the absolute burden is generally increasing. The substantial regional differences in BPH burden underscore its widespread impact and potential controllability, indicating the need for more targeted healthcare efforts to address the growing BPH burden.
Ethnobotanical Survey of Cerrado Plants Used as Antihypertensives for the Elderly in Rondonópolis, MT, Brazil.
Authors: Santos Gleyciany Stefany Alves , Piza Paulo Adaias , Toniasso Isabela Monteiro , Lacerda Maryland Sanchez , Melo Laura Valdiane Luz , Silva Marcondes Alves Barbosa da 
Published: December 2024

Abstract

Ethnobotany studies the aspects by plant utilization by societies, encompassing principally the medicinal utility. Consequently, it helps in the development bioactive natural products. This ethnobotanical study was focused medicinal plants to the Cerrado, as used by elderly in Rondonópolis-MT, Brazil for hypertension treatment. Initially, two institutions supporting elderly in Rondonópolis were chosen: Recanto do Idosos and the Associação de Apoio à Terceira Idade. A questionnaire, comprising open and closed questions covering personal data, general plant-use information, and knowledge about species with anti-hypertensive properties, was used. Plants cited as anti-hypertensive and originating from the Cerrado were collected and submitted for taxonomic classification. A total of 41 elderly participated in the study, with 60.08% women, and 39.02% having incomplete elementary education. Notably, mothers played a prominent role, accounting for approximately 38.71% of the influence on plant use. Among the respondents, 58.06% believed that plants exhibit a moderate effect, while 100% claimed that plants do not cause adverse effects, and 93.5% affirmed the transmission of this knowledge to younger generations. In conclusion, 11 medicinal plants with anti-hypertensive activity were identified, with only 4 classified as belonging to the Cerrado post-bibliographic research. Following collection and botanical classification, the scientific names obtained were: Costus arabicus L.; Solanum paniculatum L.; Acrocomia aculeata (Jacq.) Lodd. Ex Mart.; and Bixa orellana L. The limited number of plants mentioned raises concerns about the potential loss of traditional knowledge about plant among elderly. Hence, undertaking new ethnobotanical investigations becomes crucial for documenting this culturally significant knowledge.
A Girl with Immunoglobulin G4-Related Kidney Disease; A case report
Authors: Venugopal Sreeram , Gopalakrishnan Natarajan , Thanigachalam Dinesh Kumar , Abraham Anila 
Published: December 2024

Abstract

Introduction
: Immunoglobulin G4-Related Disease (IgG4-RD) is a rare condition, particularly in the pediatric population, and it is an uncommon cause of Acute Tubulointerstitial Nephritis (ATIN).
Case Presentation:
A renal biopsy of a girl with sudden onset renal failure revealed ATIN in the absence of any identifiable infections, medications, or obvious autoimmune disorders. Immunohistochemical staining of the renal biopsy showed IgG-positive plasma cells within the interstitium, along with elevated plasma IgG4 levels. These findings led to a diagnosis of immunoglobulin IgG4-RD of the kidney, a rare finding in this age group. The girl responded well to a combination therapy of steroids and Mycophenolate Mofetil (MMF).
Conclusion
IgG4-RD should be considered in the differential diagnosis of ATIN, especially with interstitial plasma cell infiltration and fibrotic changes in renal biopsy.
Prevalence of Vertebral Fractures in Patients with Acromegaly: A Multicenter Cross-Sectional Study
Authors: Almalki Mussa H. , Almohaya Mohammed , Aziz Faisal , AlDahmani Khaled M. , Alashgar Lolwah , Ekhzaimy Aishah , Mahzari Moeber 
Published: December 2024

Abstract

Introduction: Acromegaly is a chronic disease characterized by excessive growth hormone (GH) secretion, leading to high insulin-like growth factor 1 (IGF-1) levels. It results in systemic complications, including vertebral fractures (VFs), which impair quality of life. Bone mineral density (BMD) may not fully capture fracture risk, warranting further research into alternative predictors and mitigation strategies.
Objectives: This study aimed to assess the prevalence of VFs in patients with acromegaly and identify associated risk factors.
Methods: A multicenter, cross-sectional study was conducted at three centers in Saudi Arabia and one in the UAE. Data for patients with acromegaly evaluated between 2010 and 2024 were reviewed.
Results: The cohort consisted of 101 patients (63% male), with a median age of 45 years and an average disease duration of 7 years. VFs were present in 9.9% of patients, predominantly affecting the lumbar spine and were generally mild. Although not statistically significant, patients with VFs had longer disease durations and higher IGF-1 levels. BMD measurements did not significantly differ between those with and without VFs; however, patients with VFs showed a trend toward lower trabecular bone score (TBS), suggesting compromised bone microarchitecture.
Conclusion: This study underscores the importance of comprehensive bone health assessment in patients with acromegaly, incorporating BMD, TBS, and hormonal evaluations. Continuous long-term monitoring of bone health parameters is necessary in this population. Future research is needed to delineate risk factors associated with VFs.
Evaluation of Panoramic Radiographic Measurements in Subadults Aged According to Age and Sex
Authors: Okkesim Alime , Erhamza Turkan Sezen 
Published: December 2024

Abstract

Background
The study utilized panoramic radiography measurements to evaluate age and sex in individuals aged 9–17 within the Turkish population. The study aimed to address the gaps in the literature by focusing on adolescence and pre-adolescence and investigating the impact of puberty on mandibular parameters in both sexes. Through this inquiry, this study sought to contribute to the refinement of forensic anthropological methodologies, particularly in cases involving subadult mandibular remains of unknown age or sex.
Methods
The sample comprised 631 patients, including 326 females and 305 males. Panoramic radiographs were obtained using a specific model with standardized exposure parameters. Participants were 9–17 years of age. Various mandibular parameters were measured, including the bigonial width, condylar height, ramus breadth, coronoid height, projective ramus height, mandibular cortical index, gonial angle, antegonial angle, and antegonial height. The data were analyzed using descriptive statistics, the Mann-Whitney U test, and Univariate Linear Regression Analysis.
Results
There were significant differences in several mandibular parameters between males and females across different age groups. For instance, females generally exhibited higher values in condylar height, coronoid height, projective height of ramus, mental index, and mandibular cortical index, while males showed higher values in min ramus breadth, antegonial measurements, and bigonial width. However, the gonial angle did not show significant differences between sexes. The regression analysis showed that condylar height and coronoid height were significant predictors of age in both males and females.
Conclusions
Panoramic radiography measurements are reliable tools for age and sex determination in subadult individuals within the Turkish population. The findings underscore the importance of mandibular parameters in forensic anthropology and suggest the potential of panoramic radiography in aiding forensic investigations and identification processes. Further large-scale and multicenter studies are recommended to validate the applicability and accuracy of these parameters across diverse populations.
Dissecting the Role of Cellular Senescence in Meningioma Recurrence: Integrative Bioinformatics and Elastic Network Modeling
Authors: Huang Jian-huang , Chen Yao , Kang Yuan-bao , Lin Cai-hou 
Published: December 2024

Abstract

Background
Cellular senescence is intimately tied to tumorigenesis and progression, yet its exploration in meningiomas remains inadequate. In this study, we aim to unravel the role of cellular senescence-associated genes (CSA-genes) in meningioma recurrence and identify potential diagnostic markers and therapeutic targets.
Methods
We analyzed GSE136661 and GSE173825 datasets to identify CSA-signature genes through differential expression analysis, weighted gene co-expression network analysis, protein-protein interaction network construction, and elastic net regression modeling. Functional enrichment, immune cell infiltration using CIBERSORT, and transcription factor prediction were performed. Potential drugs were screened using Enrichr database.
Results
CDK1, FOXM1, MYBL2, and BIRC5 emerged as key CSA-genes related to cell cycle and DNA damage. Recurrent meningiomas showed immune heterogeneity, with CSA-genes correlating with immune infiltration and checkpoint molecules. E2F1 was predicted as a regulator. Dasatinib and Rapamycin showed promising anti-meningioma potential.
Conclusion
Our findings highlight crucial genes and pathways in meningioma recurrence, introducing novel therapeutic candidates. These findings pave new avenues for further elucidating meningioma recurrence mechanisms and developing innovative treatments.
A risk prediction model for nausea and vomiting after TACE : a cross-sectional study
Authors: Dai Yali , Zeng Mengting , He Hong , Cai Miao 
Published: December 2024

Abstract

Purpose
It was found that 52.5% of patients experienced nausea and vomiting after Transcatheter Arterial Chemoembolization (TACE), but there are no models that predict this risk. In this study, we investigated the factors associated with nausea and vomiting after TACE and developed a predictive model to predict these adverse events.
Method
The study will include 401 patients who will be randomly assigned to the training group and validation group in a 7:3 ratio. An analysis of logistic regression was used to identify predictors and build a risk prediction model. Model performance was evaluated using the Area Under Curve (AUC), Calibration Curve, and Decision Curve Analysis (DCA).
Results
This study ultimately included 401 patients for TACE, of whom 132(32.92%) patients experienced nausea and vomiting. There were independent risk prediction models for the history of vomiting, the preventive use of antiemetic, pain, and platinum and anthracene. Testing and training groups had AUCs of 0.839 and 0.742, respectively. They had calibration curves with P = 0.208 and P = 0.482, respectively. The DCA curves showed that the model had good clinical benefit at threshold probabilities greater than 20%.
Conclusion
The prediction model has good discriminatory ability, accuracy evaluation and clinical efficacy and is presented by nomogram with strong operability, contributing to for clinical medical staff to improve the management of TACE.
Recombinant expression of a new antimicrobial peptide composed of hBD-3 and hBD-4 in Escherichia coli and investigation of its activity against multidrug-resistant bacteria
Authors: Yan Han , Ning Nianzhi , Yu Wenjing , Zhang Liangyan , Cao Binwang , Li Deyu , Zhang Xingxiao , Wang Hui 
Published: December 2024

Abstract

Human β-defensin(HBD), a short cationic peptide found in humans, exhibits broad-spectrum antimicrobial activity, making it a significant candidate for antimicrobial drug development. In previous studies, we fused human β-defensin 3 and human β-defensin 4 to create a chimeric human β-defensin, named H4, with enhanced antimicrobial activity and improved salt stability. However, due to the relatively large number of amino acids in H4, the cost of its chemical synthesis is significant. The objective of this study is to explore a method for producing H4 using a prokaryotic expression system to reduce its manufacturing costs. We first optimized the codons of H4 to make it suitable for expression in prokaryotic systems. Subsequently, the optimized Open Reading Frame of H4 was cloned into the pET32a vector and combined with thioredoxin and enterokinase cleavage sites to reduce its toxicity to prokaryotic cells. The resulting plasmid was introduced into E. coli BL21, and then the expression was induced using IPTG to produce a fusion protein (TrxA-EK-H4). TrxA-EK-H4 was isolated using a nickel column, and then EK cleavage was performed to generate unlabeled H4. Our results indicated that TrxA-EK-H4 can be cleaved by EK when using a nickel column, but it does not undergo precise cleavage in Tris-HCl buffer. The antimicrobial assays showed that the recombinant H4 exhibited similar bactericidal activity against multidrug-resistant Acinetobacter baumannii as the chemically synthesized H4. In summary, this study has developed a prokaryotic expression system for H4. Our approach offers a novel experimental concept for the future purification of similar proteins that are difficult to cleave effectively.