bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knockdown of hsa_circ_0074298 suppressed the progression of pancreatic cancer by targeting the miR-519/SMOC2 axis
Authors: Chen Hong , Xu Dafang , Wang Lishan , Zhou Jiahua 
Published: February 2020

Abstract
BACKGROUND: Pancreatic cancer (PC) is among the most malignant tumors in digestive system because of its fast progression, metastasis as well as resistance to chemotherapy. Former studies found that circRNAs that differentially expressed were correlated to progression and gemcitabine (GEM)-resistance in PC, and hsa_circ_0074298 aberrant expression played a role in PC progression, but the regulatory mechanism was unclear.
METHODS: Rt-qPCR was used to detect hsa_circ_0074298 expression in PC cell lines. The CCK8, colony formation, and Transwell assays were used to evaluate the effect of hsa_circ_0074298 on PC cell migration and proliferation. Bioinformatics and luciferase reporter experiments were used to study the regulatory mechanism. Nude mouse xenografts were generated to examine the effect of hsa_circ_0074298 on tumor growth.
RESULTS: The present study showed that hsa_circ_0074298 expression increased significantly in both PC cell lines and PC tissues. Downregulation of hsa_circ_0074298 caused a significant decrease in PC cell proliferation and in nude mouse xenograft growth. Hsa_circ_0074298 silencing also increased chemotherapy sensitivity to GEM. Bioinformatics analysis indicated that hsa_circ_0074298 was a miR-519 sponge and that the SMOC2 gene was a miR-519 target. MiR-519 downregulation or SMOC2 overexpression restored cell proliferation, migration, and chemoresistance after hsa_circ_0074298 silencing. The dual luciferase reporter assay showed that hsa_circ_0074298 interacted with miR-519, and miR-519 binding to the SMOC2 3′-UTR, then suppressed posttranscriptional SMOC2 expression.
CONCLUSIONS: Taken together, hsa_circ_0074298 functioned as a tumor promoter through novel miR-519/SMOC2 axis, highlighting its possibility as a potential therapy for PC.
Occurrence of anterior uveitis in patients with spondyloarthritis treated with tumor necrosis factor inhibitors: comparing the soluble receptor to monoclonal antibodies in a large observational cohort
Authors: khoury Gisèle , Morel Jacques , Combe Bernard , Lukas Cédric 
Published: February 2020

Abstract
Background: The objective of this study was to compare in real life the occurrence of anterior uveitis in patients with spondyloarthritis (SpA), including psoriatic arthritis (PsA), treated with the soluble-receptor etanercept (ETA) or monoclonal antibodies (mAb). Methods: This was an observational, retrolective study. Patients with SpA who were prescribed anti-TNF agents between 2000 and 2014 were included. The risk of uveitis was interpreted qualitatively (number of subjects with at least one uveitis) and quantitatively (number of uveitis flares for each individual). Models were adjusted for propensity score of receiving preferentially mAbs or ETA. Results: 429 patients were included (302 with SpA and 127 with PsA); 203 received a Mab and 226 ETA as a first TNF-a inhibitor. Probability of uveitis occurring during the first year of treatment was lower with ETA than mAbs but not significantly (odds ratio 0.94 [95% confidence interval 0.35; 2.54], p=0.90, on qualitative analysis and relative risk 0.62 [0.26; 1.46], p=0.27, on quantitative analysis) after adjustment for the propensity score. The over-time risk of uveitis was numerically higher with ETA than mAbs, but the differences were not statistically significant. Conclusion: In this observational study, the risk of uveitis in patients with SpA does not appear to be greater with ETA than mAbs treatment. The occurrence of uveitis in patients receiving an anti-TNF-a agent seems linked more to history of uveitis than the prescribed molecule.
Nummular Headache - A case report of a rare entity
Authors: Thomas Davis , Heir Gary M. , Patil Amey G. , Soni Prutha K. 
Published: February 2020

Abstract
Background: Nummular Headache is a rare type of chronic primary headache wherein the pain is usually located in a well-circumscribed, round or elliptical shaped area of the scalp. Case Report: A 26-year-old female presented to the Center for TMD/ OFP with the chief complaint of chronic persistent left parietal headache, for more than 10 years, which has been worsening over the past few days with no specific identifiable trigger. The medical history is non- contributory. The primary diagnosis is Nummular Headache. Conclusion: A comprehensive history is the first step to achieve an accurate diagnosis. The diagnosis of Nummular Headache is challenging and confusing due to the rare occurrence. An examination must include imaging and is detrimental in eliminating other underlying pathologies. The diagnosis is made from distinct clinical features after exclusion of all other entities. Keywords: Nummular headache, Rare primary headaches, Coin-shaped headache, Chronic Primary headache, Epicranial headache.
Benefits of a community based interdisciplinary learning exposure: A qualitative study of the One Health approach in teaching at Makerere University, Uganda
Authors: Buregyeya Esther , Atusingwize Edwinah , Nsamba Peninah , Nalwadda Christine , Osuret Jimmy , Kalibbala Patrick , Nuwamanya Ronald , Ssekamatte Tonny , Wakabi Timothy , Bikaako Winnie , Yawe Agnes , Naigaga Irene , Kagarama Juvenal , Kabasa John David , Bazeyo William 
Published: February 2020

Abstract
Makerere University implemented a One Health Institute (OHI) in 2016 involving undergraduate students selected from different disciplines. The students were first taken through theoretical principles in One Health followed by a field attachment in communities. The field attachment aimed to expose students to experiential educational opportunities in the communities in a One Health approach. In this paper, we present students’ experiences and their contributions to the communities of attachment.MethodsThis was a cross-sectional study, utilizing qualitative data collection methods. The study involved students who participated in the OHI field attachment and community members in Western Uganda. Four focus group discussions (FGDs) and four in-depths (IDIs) were conducted among the students, while four FGDs and eight IDIs were conducted among community members. All interviews were audio-recorded, transcribed and analysed manually.ResultsThe four themes that emerged are: students’ understanding and appreciation of One Health concept, their experiences and gains from the multi-disciplinary field attachment, students’ contributions to the community, and challenges faced by the students. Students had good knowledge of One Health. They appreciated that health cannot be achieved by one discipline or sector and thus the need to collaborate across sectors. Regarding experiences and gains during the multi-disciplinary field attachment, the students appreciated that each discipline had a role to play in achieving health in the community. They appreciated the training sighting skills gained in communication, team work and collaboration. They also reported a feeling of gratitude and accomplishment because they felt they made a positive change to the community by putting in place interventions to address some of the community challenges. Similarly, the communities appreciated the students’ contribution in solving their health challenges, ranging from conducting health education to improving sanitation and hygiene.ConclusionsThrough the OHI, students gained One Health competencies including communication, teamwork, and collaboration. Adopting an interdisciplinary model in university teaching system especially during field placement would strengthen skills of collaboration, team work and communication which are critical for a multi-disciplinary approach which is needed among the future workforce in order to solve the current health challenges.
Descriptive epidemiology of objectively-measured, free-living sleep parameters in a rural African setting.
Authors: Cook Ian , Mohlabe Matlawa , Alberts Marianne 
Published: February 2020

Abstract
Objectives

To investigate the descriptive nature of objectively-measured, free-living sleep quantity and quality, and the relationship to adiposity, in a rural African setting in 145 adults (≥40 years, female: n=104, male: n=41). Wrist-mounted, tri-axial accelerometry data was collected over nine days. Measures of sleep quantity and quality, and physical activity were extracted from valid minute-by-minute data. From stature, body mass and waist circumference, body-mass-index and conicity index were calculated. Self-reported data included behavioural, health and socio-demographic variables. Community consultation followed the quantitative data analyses, for validation and interpretation of findings.

Results

Females had more nocturnal sleep than males (7.2 vs. 6.8 hours/night, p=0.0464) while males recorded more diurnal sleep time (p=0.0290). Wake after sleep onset and number of awakenings were higher in females, and sleep efficiency was higher in males (p≤0.0225). Sleep indices were generally similar between weekdays and weekends, except for sleep fragmentation index (p=0.0458). Sleep quantity, but not sleep quality was independently and inversely associated with adiposity (p=0.0453). Physical activity and morbidity measures were significantly and consistently associated with sleep and adiposity measures (p<0.0458). The qualitative data explained some of the unexpected associational directions of the independent variables correlated with sleep variables.
Percutaneous intra-articular injections of ropivacaine, and tranexamic acid and removal of the drainage tube one day after total knee arthroplasty as a component of enhanced recovery after surgery: a randomized control trial
Authors: Pei Lijia , Li Xiaoyang , Liu Yang , Wang Xuyi , Ding Hai , Wang Zhiyan , zhou xinshe 
Published: February 2020

Abstract
Background: Intraoperative injections of ropivacaine and tranexamic acid have an increasing role as part of analgesia and haemostasis for enhanced recovery after Total Knee Arthroplasty. However, drug efficiency gradually disappears one day after surgery. Drainage tube placed for too long can cause various complications. This study evaluates whether the postoperative injections of ropivacaine, and tranexamic acid and removal of the drainage tube one day would promote recovery after TKA. Methods: A total of 80 participants were randomly assigned to two groups. One group was treated with additional intra-articular injections at 08:30 on postoperative day 1 and the removal of drainage tube simultaneously (Trial Group). Another group was dealt with no injection and removal of the drainage tube responding to the amount of drainage fluid (Control Group). The multi-drug solution included 100 mg ropivacaine, and 1 g tranexamic acid, and 10 mL saline. The primary outcomes assessed included visual analog scale score, length of hospital stay after surgery, the circumference of the knee, total blood loss volume. Results: The group that received an additional intra-articular injection of ropivacaine had a significantly lower area under the curve for visual analog scale score than the no injection group at rest (424 ± 32 compared with 515 ± 39, p = 0.003). The Trial Group can reduce the length of hospital stay after surgery over the Control Group (7.1 ± 3.1 days compared with 8.7 ± 3.2 days, p = 0.003). The group that received an additional intra-articular injection of tranexamic acid have no difference in total blood loss volume & the circumference of knee joint over the no additional intra-articular injection group (910.6 ± 385.3 mL compared with 974.8 ± 408.2 mL, p=0.47 & 38.9 ±5.4cm compared with 38.8±2.4cm，p=0.91). Conclusions: Intra-articular injection of ropivacaine, and tranexamic acid and removal of the drainage tube one day after TKA can further promote recovery after TKA. Keywords: enhanced recovery after surgery, total knee arthroplasty, tranexamic acid, ropivacaine
High-viscosity bone cement for vertebral compression fractures: a prospective study on intravertebral diffusion of bone cement
Authors: wang meiyong , jin qunhua 
Published: February 2020

Abstract
Objective Bone cement leakage causes severe complication following percutaneous vertebroplasty. This study probed the diffusion and leakage status of bone cement injected within diverged time duration, so as to find the optimal injection time for bone cement. Methods A total of 70 patients with osteoporotic vertebral compression fractures with a symptom of low back pain, who underwent treatment at hospital were enrolled in this study. Patients were randomized into three groups: <180 s, 180-300, and >300 s of injection time duration from the beginning to the completion of the injection. The scenarios of vertebral bone cement leakage and diffusion were inspected using postoperative CT. Results The diffusion coefficient was higher in group A than in group B whereas it was higher in group B than in group C, but without statistical significance among the three groups. The leakage rate was without statistical significance among the three groups. The injection time of bone cement was negatively correlated with the diffusion coefficient, at the correlation coefficient of -0.253. Conclusions The diffusion coefficient of high-viscosity bone cement is likely negatively correlated with the injection time, and the leakage rate of high-viscosity bone cement probably does not reduce with the prolongation of injection time.
IL-37 Gene Modification Enhances the Protective Effects of Mesenchymal Stromal Cells on Intestinal Ischemia Reperfusion Injury
Authors: Kong Dejun , Hu Yonghao , Li Xiang , Yu Dingding , Li Hongyue , Zhao Yiming , Qin Yafei , Jin Wang , Zhang Baoren , Wang Bo , Wang Hongda , Li Guangming , Wang Hao 
Published: February 2020

Abstract
Background: Ischemia reperfusion injury (IRI) is the major cause of intestinal damage in clinic. Although either mesenchymal stromal cells (MSCs) or interleukin 37 (IL-37) showed some beneficial roles to ameliorate IRI, their effects are limited. In this study, the protective effects of IL-37 gene-modified MSCs (IL-37-MSCs) for better prevention of intestinal IRI are investigated.Methods: Intestinal IRI model was established by occluding the superior mesenteric artery for 30min and then reperfusing for 72 hours in rats. Forty adult male SD rats were randomly divided into sham control, IL-37-MSC-treated, MSC-treated, recombinant IL-37 (rIL-37)-treated and untreated groups. Intestinal damage was assessed by H&E staining. The levels of gut barrier function factors (diamine oxidase and D-Lactate) and inflammation reactivity cytokine IL-1β were assayed by ELISA. The expressions of tissue damage-related NLRP3 inflammasome and relative proteins including clevead caspase-1, IL-1β and IL-18 were detected by western blot. As downstream of IL-1β and IL-18, the mRNA levels of proinflammatory mediators IL-6 and TNF-α were determined by qPCR. Data were analyzed by one-way analysis of variance among groups.Results: IL-37-MSCs were able to migrate to the damaged tissue and significantly inhibit intestinal IRI. As compared with MSCs or rIL-37 monotherapy group, IL-37-MSC treatment not only improved gut barrier function but also decreased local and systemic inflammation reactivity cytokine IL-1β level in IRI rats. In addition, tissue damage-related NLRP3 and relative proteins (cleaved caspase-1, IL-1β and 4 IL-18) were significantly decreased in IRI rats treated with IL-37-MSCs. Furthermore, IL-1β and IL-18 related proinflammatory mediators IL-6 and TNF-α mRNA expressions were markedly decreased following IL-37-MSC treatment.Conclusion: The results suggest that IL-37 gene modification significantly enhance the protective effects of MSCs against intestinal IRI. In addition, NLRP3-related signaling pathways could be associated with IL-37-MSC mediated protection.
Targeting 14-3-3ζ overcomes resistance to EGFR-TKI in lung adenocarcinoma via BMP2/Smad/ID-1 signaling
Authors: Cui Jinfang , Song Yang , Han Xuejiao , Hu Jing , Chen Yanbo , Chen Xuesong , Xu Xiaomin , Xing Ying , Lu Hailing , Cai Li 
Published: February 2020

Abstract
Background: 14-3-3ζ protein which acts as a putative oncoprotein has been found to promote proliferation, metastasis and chemoresistance of cancer cells in several cancers including lung adenocarcinoma (LUAD), however, its significance in epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) resistance remains unknown. Methods: The Cancer Genome Atlas (TCGA) database was used to determine 14-3-3ζ expression in pan-cancer and LUAD. 14-3-3ζ and ID1 expression was then examined in clinical LUAD samples by immunohistochemistry (IHC). Lentiviral transfection with 14-3-3ζ-specific shRNA was used to establish stable 14-3-3ζ knockdown gefitinib resistant PC9 (PC9/GR) and H1975 cell lines. The effect of 14-3-3ζ knockdown on reversing EGFR-TKI resistance was determined in vitro by CCK-8, wound healing, transwell assays and flow cytometry. A xenograft tumor model was established to evaluate the role of 14-3-3ζ in EGFR-TKI resistance. Microarray analysis results showed the multiple pathways regulated by 14-3-3ζ-shRNA. Results: In the present study, we firstly demonstrated that 14-3-3ζ expression was elevated and predicted unfavourable prognosis in pan-cancer including LUAD based on TCGA. In addition, high 14-3-3ζ expression was significantly associated with advanced T stage, TNM stage, present of lymph node metastasis and, importantly, poor treatment response to EGFR-TKI in LUAD patients with EGFR-activating mutations. 14-3-3ζ shRNA significantly sensitized EGFR-TKI-resistant human LUAD cells to geﬁtinib and, notably, reversed epithelial-to-mesenchymal transition (EMT). BMP signaling activation was decreased in EGFR-TKI resistant cells followed by 14-3-3ζ depletion in microarray analysis, which was further validated by Western blot analysis. Furthermore, the expression of 14-3-3ζ positively correlates with ID1 expression in human EGFR-mutant LUAD patient samples. In vivo , 14-3-3ζ shRNA and gefitinib treatment resulted in a significant reduction in the tumor burden compared to that treated with gefitinib alone. Conclusion: Our work uncovers a hitherto unappreciated role of 14-3-3ζ in EGFR-TKI resistance. This study might provide a potential therapeutic approach for treating LUAD patients harboring EGFR mutations.
The use of Lactobacillus plantarum 299v (DSM 9843) in patients with cancers receiving home enteral nutrition – study protocol for a randomized, double-blind, and placebo-controlled trial
Authors: Karolina Kaźmierczak-Siedlecka , Folwarski Marcin , Skonieczna-Żydecka Karolina , Ruszkowski Jakub , Makarewicz Wojciech 
Published: February 2020

Abstract
Background Nutritional treatment is one of the most important components of multidisciplinary anti-cancer therapy. Home enteral nutrition is considered a safe procedure, however, may be associated with the risk of side effects, such as nausea, vomiting, abdominal pain, and diarrhoea. It is uncertain whether diarrhoea is the result of the enteral formula or gut dysbiosis. One of the methods which may be used to alter the composition of gut microbiota is the administration of a probiotic strain. Lactobacillus plantarum 299v may be used as a supportive therapy in patients suffering from irritable bowel syndrome and Clostridium difficile infection. Therefore, the primary aim of this study is to determine the effect of Lactobacillus plantarum 299v on nutritional status of cancer patients receiving home enteral nutrition. The secondary aims are to evaluate the role of this probiotic strain in the improvement of tolerance of enteral formula and patients’ quality of life. Methods Forty patients with cancer receiving home enteral nutrition will be enrolled in this clinical trial and randomized to receive one capsule of Lactobacillus plantarum 299v (Sanprobi IBS®) twice a day or placebo for 12 weeks in a double-blind manner. Laboratory tests (the level of albumin, total protein, transferrin, and total lymphocyte count), anthropometric parameters (body mass, the content of fat mass, muscle mass, and total body water), Nutritional Risk Screening (NRS 2002), tolerance of enteral nutrition as well as quality of life will be measured. Measurements will be obtained at the baseline and after 4 and 12 weeks of treatment. Discussion It is expected that the Lactobacillus plantarum 299v will provide beneficial effects, such as maintenance or improvement of nutritional status, enteral formula tolerance, and quality of life of cancer patients receiving home enteral nutrition. Trial registration ClinicalTrials.gov Identifier: NCT03940768
Quantitative assessment of German Holstein dairy cattle colostrum and impact of thermal treatment on quality of colostrum viscosity and immunoglobulins
Authors: Hassan Abdulwahed Ahmed , Ganz Sebastian , Schneider Florian , Wehrend Axel , Khan Izhar U.H. , Failing Klaus , Bülte Michael , Abdulmawjood Amir 
Published: February 2020

Abstract
Objective: This study aimed to determine the color, fat, viscosity, IgG concentration, %Brix and refractive index of fresh postpartum colostrum of German Holstein dairy cattle and assess the impact of different thermal treatments on the visual and dynamic viscosity, in association to IgG concentration, of colostrum that can be used for pasteurization process.Results: Of the total 40 fresh postpartum colostrum, the color of colostrum (white-pale yellow to yellow and dark-yellowish), fat (1.4 - 8.2 100 g-1), IgG (4 - 116 mg mL-1), %Brix (8.5 - 35.4%), refractive index (1.3454 - 1.3905 nD), visual (ranging from watery to liquid and thick) and dynamic (4.9 - 219 cp) viscosity, were recorded. Statistical analysis between visual and dynamic viscosity of fresh colostrum showed significant correlation coefficients (rs = 634). Moreover, a significant correlation between viscosity and three IgG concentrations was observed. Heat-treated colostrum showed dynamic viscosity ranged from 25 to 3066 cP, where dynamic viscosity of colostrum before- and after heat-treatment showed no significant correlation. Treated colostrum containing IgG concentration ≤80 mg mL-1 at 60°C/60 min and ≤68 mg mL-1 at 63.5°C/30 min showed no significant change in the viscosity and can successfully be applied for pasteurization of first postpartum colostrum.
Lessons learned through piloting a community-based SMS referral system for common mental health disorders used by female community health volunteers in rural Nepal
Authors: Bhardwaj Anvita , Subba Prasansa , Rai Sauharda , Bhat Chaya , Ghimire Renasha , Jordans Mark , Green Eric , Vasudevan Lavanya , Kohrt Brandon 
Published: February 2020

Abstract
Objectives: The Community Informant Detection Tool (CIDT) is a paper-based proactive case detection strategy with evidence for improving help-seeking behavior by patients for mental health disorders. Key implementation barriers for the paper-based CIDT include delayed reporting of cases and lack of active follow up. We used mobile phones and structured short message system to improve timeliness of case reporting, follow up and case record keeping. Thirty-six lay health workers piloted this mobile CIDT (mCIDT) for three months in 2017 in rural Nepal.Results: Eight cases were identified by lay health workers using mCIDT, but only two of these cases engaged with health services post-referral. Accuracy with the mCIDT was considerably lower compared to the paper-based CIDT. Higher education levels of lay health workers was associated with greater mCIDT accuracy. Qualitative findings revealed new implementation challenges among cases not following through on referrals due to perceived lack of staff at health facilities, assumptions among lay health workers that all earthquake-related mental health needs had been met, and lack of financial incentives for use of mCIDT. Based on study findings, we present recommendations for introducing a new technology in low resource health systems.