bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asymmetric Development of Auroral Surges in the Northern and Southern Hemispheres
Authors: Uchida H. A. , Kataoka R. , Kadokura A. , Murase K. , Yukimatu A. S. , Miyoshi Y. , Shiokawa K. , Ebihara Y. , Hosokawa K. , Matsuoka A. , Kurita S. , Fujita S. , Shinohara I. 
Published: June 2020

AbstractSimultaneous eastward and westward traveling surges were observed at Tjörnes, Iceland, and Syowa station, Antarctica, respectively. Several remarkable differences were identified. (1) The position of the initial bright spot was shifted by 1.7 to 2.3 MLT between both hemispheres. (2) The surges differ in traveling speed between the eastward traveling surge (6.5 km s−1) and the westward traveling surge (1.3 km s−1). (3) The Arase satellite was located on a geomagnetic field line connecting both ground stations and observed a significant excess in westward component of the magnetic field, which is consistent with the large shifts of the initial bright spots in both hemispheres. (4) The background Hall current flows eastward (Northern Hemisphere) and westward (Southern Hemisphere). The observed north‐south asymmetry of the traveling surges suggests that the ionosphere can play an essential role in controlling the fundamental spatiotemporal development of auroras in both hemispheres.
Statistical Relations Between Auroral Electrical Conductances and Field‐Aligned Currents at High Latitudes
Authors: Robinson R. M. , Kaeppler Stephen R. , Zanetti Larry , Anderson Brian , Vines Sarah K. , Korth Haje , Fitzmaurice Anna 
Published: June 2020

AbstractField‐aligned currents from the Active Magnetosphere and Planetary Electrodynamics Response Experiment (AMPERE) were combined with simultaneous and coincident observations of ionospheric conductivities made by the Poker Flat Incoherent Scatter Radar (PFISR) in Alaska for 20 geomagnetically active days. The height‐integrated conductivities (conductances) were determined from the electron densities measured by the radar between 80 and 200 km altitude. Binning and averaging the data by field‐aligned current density and magnetic local time, we find that the currents correlate with conductances in both upward and downward current regions over some magnetic local times. The strongest correlation is seen in the late evening and morning sectors, with the Hall conductances two to three times larger than the Pedersen conductances for the same values of the field‐aligned current. The observed correlations reflect the mean energy of auroral precipitation, the contributions from electrons and protons to producing enhanced conductances, and the availability of charge carriers on auroral field lines. We apply linear fitting and smoothing to the correlations to construct an empirical model for specifying auroral conductances globally from AMPERE field‐aligned current maps. The energy fluxes from precipitating particles derived from the model conductances compare well with those derived using AMPERE data combined with satellite‐based measurements of far ultraviolet emissions, suggesting the results obtained at Poker Flat may be applicable to all high latitude locations. The ability to estimate conductances from AMPERE field‐aligned current maps provides the means to develop a global conductance model for the auroral ionosphere.
Magmatic Intrusions Control Io's Crustal Thickness
Authors: Spencer Dan C. , Katz Richard F. , Hewitt I. J. 
Published: June 2020

AbstractIo, the most volcanically active body in the solar system, loses heat through eruptions of hot lava. Heat is supplied by tidal dissipation and is thought to be transferred through the mantle by magmatic segregation, a mode of transport that sets it apart from convecting terrestrial planets. We present a model that couples magmatic transport of tidal heat to the volcanic system in the crust, in order to determine the controls on crustal thickness, magmatic intrusions, and eruption rates. We demonstrate that magmatic intrusions are a key component of Io's crustal heat balance; around 80% of the magma delivered to the base of the crust must be emplaced and frozen as plutons to match rough estimates of crustal thickness. As magma ascends from a partially molten mantle into the crust, a decompacting boundary layer forms, which can explain possible observations of a high‐melt‐fraction region.
Pulmonary hypertension in bronchopulmonary dysplasia
Authors: Hansmann Georg , Sallmon Hannes , Roehr Charles C. , Kourembanas Stella , Austin Eric D. , Koestenberger Martin ,   for the European Pediatric Pulmonary Vascular Disease Network (EPPVDN)
Published: June 2020

Abstract
Bronchopulmonary dysplasia (BPD) is a major complication in prematurely born infants. Pulmonary hypertension (PH) associated with BPD (BPD-PH) is characterized by alveolar diffusion impairment, abnormal vascular remodeling, and rarefication of pulmonary vessels (vascular growth arrest), which lead to increased pulmonary vascular resistance and right heart failure. About 25% of infants with moderate to severe BPD develop BPD-PH that is associated with high morbidity and mortality. The recent evolution of broader PH-targeted pharmacotherapy in adults has opened up new treatment options for infants with BPD-PH. Sildenafil became the mainstay of contemporary BPD-PH therapy. Additional medications, such as endothelin receptor antagonists and prostacyclin analogs/mimetics, are increasingly being investigated in infants with PH. However, pediatric data from prospective or randomized controlled trials are still sparse. We discuss comprehensive diagnostic and therapeutic strategies for BPD-PH and briefly review the relevant differential diagnoses of parenchymal and interstitial developmental lung diseases. In addition, we provide a practical framework for the management of children with BPD-PH, incorporating the modified definition and classification of pediatric PH from the 2018 World Symposium on Pulmonary Hypertension, and the 2019 EPPVDN consensus recommendations on established and newly developed therapeutic strategies. Finally, current gaps of knowledge and future research directions are discussed.

Impact

PH in BPD substantially increases mortality. Treatment of BPD-PH should be conducted by an interdisciplinary team and follow our new treatment algorithm while still kept tailored to the individual patient.

We discuss recent developments in BPD-PH, make recommendations on diagnosis, monitoring and treatment of PH in BPD, and address current gaps of knowledge and potential research directions.

We provide a practical framework, including a new treatment algorithm, for the management of children with BPD-PH, incorporating the modified definition and classification of pediatric PH (2018 WSPH) and the 2019 EPPVDN consensus recommendations on established and newly developed therapeutic strategies for BPD-PH.
Unusual marine cyanobacteria/haptophyte symbiosis relies on N2 fixation even in N-rich environments
Authors: Mills Matthew M , Turk-Kubo Kendra A , van Dijken Gert L , Henke Britt A , Harding Katie , Wilson Samuel T , Arrigo Kevin R , Zehr Jonathan P 
Published: June 2020

Abstract
The microbial fixation of N2 is the largest source of biologically available nitrogen (N) to the oceans. However, it is the most energetically expensive N-acquisition process and is believed inhibited when less energetically expensive forms, like dissolved inorganic N (DIN), are available. Curiously, the cosmopolitan N2-fixing UCYN-A/haptophyte symbiosis grows in DIN-replete waters, but the sensitivity of their N2 fixation to DIN is unknown. We used stable isotope incubations, catalyzed reporter deposition fluorescence in-situ hybridization (CARD-FISH), and nanoscale secondary ion mass spectrometry (nanoSIMS), to investigate the N source used by the haptophyte host and sensitivity of UCYN-A N2 fixation in DIN-replete waters. We demonstrate that under our experimental conditions, the haptophyte hosts of two UCYN-A sublineages do not assimilate nitrate (NO3−) and meet little of their N demands via ammonium (NH4+) uptake. Instead the UCYN-A/haptophyte symbiosis relies on UCYN-A N2 fixation to supply large portions of the haptophyte’s N requirements, even under DIN-replete conditions. Furthermore, UCYN-A N2 fixation rates, and haptophyte host carbon fixation rates, were at times stimulated by NO3− additions in N-limited waters suggesting a link between the activities of the bulk phytoplankton assemblage and the UCYN-A/haptophyte symbiosis. The results suggest N2 fixation may be an evolutionarily viable strategy for diazotroph–eukaryote symbioses, even in N-rich coastal or high latitude waters.
Atypical processing of tones and phonemes in Rett Syndrome as biomarkers of disease progression
Authors: Sysoeva Olga V. , Molholm Sophie , Djukic Aleksandra , Frey Hans-Peter , Foxe John J. 
Published: June 2020

AbstractDue to severe motor impairments and the lack of expressive language abilities seen in most patients with Rett Syndrome (RTT), it has proven extremely difficult to obtain accurate measures of auditory processing capabilities in this population. Here, we examined early auditory cortical processing of pure tones and more complex phonemes in females with Rett Syndrome (RTT), by recording high-density auditory evoked potentials (AEP), which allow for objective evaluation of the timing and severity of processing deficits along the auditory processing hierarchy. We compared AEPs of 12 females with RTT to those of 21 typically developing (TD) peers aged 4–21 years, interrogating the first four major components of the AEP (P1: 60–90 ms; N1: 100–130 ms; P2: 135–165 ms; and N2: 245–275 ms). Atypicalities were evident in RTT at the initial stage of processing. Whereas the P1 showed increased amplitude to phonemic inputs relative to tones in TD participants, this modulation by stimulus complexity was absent in RTT. Interestingly, the subsequent N1 did not differ between groups, whereas the following P2 was hugely diminished in RTT, regardless of stimulus complexity. The N2 was similarly smaller in RTT and did not differ as a function of stimulus type. The P2 effect was remarkably robust in differentiating between groups with near perfect separation between the two groups despite the wide age range of our samples. Given this robustness, along with the observation that P2 amplitude was significantly associated with RTT symptom severity, the P2 has the potential to serve as a monitoring, treatment response, or even surrogate endpoint biomarker. Compellingly, the reduction of P2 in patients with RTT mimics findings in animal models of RTT, providing a translational bridge between pre-clinical and human research.
SHQ1 is an ER stress response gene that facilitates chemotherapeutics-induced apoptosis via sensitizing ER-stress response
Authors: Liu Huimin , Xie Siqi , Fang Fang , Kalvakolanu Dhananjaya V. , Xiao Weihua 
Published: June 2020

AbstractSHQ1 was reported to control the biogenesis and assembly of H/ACA ribonucleoprotein particles (RNPs). It was independently isolated as a growth suppressor, GRIM1, in a genetic screen. Recent studies have indicated that SHQ1 inhibits prostate cancer growth and metastasis. SHQ1 facilitates MYC RNA splicing to promote T-acute lymphoblastic leukemia (T-ALL) development. Thus, the mechanisms of SHQ1 in cancers remain largely unknown. We report here that SHQ1 promotes tumor apoptosis and chemo-sensitivity in hepatocellular carcinoma (HCC) cells. In HCC tissues from patients, expression of SHQ1 was significantly decreased in the tumor compared to adjacent tissues. Experiments with HCC xenograft models revealed that restoring SHQ1 levels enhanced the anti-tumor activity of the endoplasmic reticulum (ER) stress inducer tunicamycin (TM) and common chemotherapy drug paclitaxel (PTX). Mechanistically, SHQ1 is an ER-stress response gene which is regulated by p50ATF6 and XBP1s through an ER stress response like element located on the SHQ1 promoter. SHQ1 interacts with the ER chaperone GRP78 to release ER sensors PERK/IRE1α/ATF6 from GRP78/ER-sensor complexes, leading to hyper-activation of unfolded protein response (UPR). In the persistent ER stress conditions of a HepG2 xenograft tumor model, SHQ1-mediated hyper-activation of ER-sensor signaling induces apoptosis. Our study thus demonstrates a SHQ1-mediated ER-stress response feedback loop that promotes tumor sensitivity to chemotherapeutics.
Expression profiling of microRNAs and isomiRs in conventional central chondrosarcoma
Authors: Parafioriti Antonina , Cifola Ingrid , Gissi Clarissa , Pinatel Eva , Vilardo Laura , Armiraglio Elisabetta , Di Bernardo Andrea , Daolio Primo Andrea , Felsani Armando , D’Agnano Igea , Berardi Anna Concetta 
Published: June 2020

AbstractConventional central chondrosarcoma (CCC) is a malignant bone tumor that is characterized by the production of chondroid tissue. Since radiation therapy and chemotherapy have limited effects on CCC, treatment of most patients depends on surgical resection. This study aimed to identify the expression profiles of microRNAs (miRNAs) and isomiRs in CCC tissues to highlight their possible participation to the regulation of pathways critical for the formation and growth of this type of tumor. Our study analyzed miRNAs and isomiRs from Grade I (GI), Grade II (GII), and Grade III (GIII) histologically validated CCC tissue samples. While the different histological grades shared a similar expression profile for the top abundant miRNAs, we found several microRNAs and isomiRs showing a strong different modulation in GII + GIII vs GI grade samples and their involvement in tumor biology could be consistently hypothesized. We then in silico validated these differently expressed miRNAs in a larger chondrosarcoma public dataset and confirmed the expression trend for 17 out of 34 miRNAs. Our results clearly suggests that the contribution of miRNA deregulation, and their targeted pathways, to the progression of CCC could be relevant and strongly indicates that when studying miRNA deregulation in tumors, not only the canonical miRNAs, but the whole set of corresponding isomiRs should be taken in account. Improving understanding of the precise roles of miRNAs and isomiRs over the course of central chondrosarcoma progression could help identifying possible targets for precision medicine therapeutic intervention.
Varietal variation and chromosome behaviour during meiosis in Solanum tuberosum
Authors: Choudhary Anushree , Wright Liam , Ponce Olga , Chen Jing , Prashar Ankush , Sanchez-Moran Eugenio , Luo Zewei , Compton Lindsey 
Published: June 2020

AbstractNaturally occurring autopolyploid species, such as the autotetraploid potato Solanum tuberosum, face a variety of challenges during meiosis. These include proper pairing, recombination and correct segregation of multiple homologous chromosomes, which can form complex multivalent configurations at metaphase I, and in turn alter allelic segregation ratios through double reduction. Here, we present a reference map of meiotic stages in diploid and tetraploid S. tuberosum using fluorescence in situ hybridisation (FISH) to differentiate individual meiotic chromosomes 1 and 2. A diploid-like behaviour at metaphase I involving bivalent configurations was predominant in all three tetraploid varieties. The crossover frequency per bivalent was significantly reduced in the tetraploids compared with a diploid variety, which likely indicates meiotic adaptation to the autotetraploid state. Nevertheless, bivalents were accompanied by a substantial frequency of multivalents, which varied by variety and by chromosome (7–48%). We identified possible sites of synaptic partner switching, leading to multivalent formation, and found potential defects in the polymerisation and/or maintenance of the synaptonemal complex in tetraploids. These findings demonstrate the rise of S. tuberosum as a model for autotetraploid meiotic recombination research and highlight constraints on meiotic chromosome configurations and chiasma frequencies as an important feature of an evolved autotetraploid meiosis.
Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging
Authors: Santos Harrisson D. A. , Zabala Gutiérrez Irene , Shen Yingli , Lifante José , Ximendes Erving , Laurenti Marco , Méndez-González Diego , Melle Sonia , Calderón Oscar G. , López Cabarcos Enrique , Fernández Nuria , Chaves-Coira Irene , Lucena-Agell Daniel , Monge Luis , Mackenzie Mark D. , Marqués-Hueso José , Jones Callum M. S. , Jacinto Carlos , del Rosal Blanca , Kar Ajoy K. , Rubio-Retama Jorge , Jaque Daniel 
Published: June 2020

AbstractOptical probes operating in the second near-infrared window (NIR-II, 1,000-1,700 nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10 mW cm−2) and doses (<0.5 mg kg−1), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing.
mTADA is a framework for identifying risk genes from de novo mutations in multiple traits
Authors: Nguyen Tan-Hoang , Dobbyn Amanda , Brown Ruth C. , Riley Brien P. , Buxbaum Joseph D. , Pinto Dalila , Purcell Shaun M. , Sullivan Patrick F. , He Xin , Stahl Eli A. 
Published: June 2020

AbstractJoint analysis of multiple traits can result in the identification of associations not found through the analysis of each trait in isolation. Studies of neuropsychiatric disorders and congenital heart disease (CHD) which use de novo mutations (DNMs) from parent-offspring trios have reported multiple putatively causal genes. However, a joint analysis method designed to integrate DNMs from multiple studies has yet to be implemented. We here introduce multiple-trait TADA (mTADA) which jointly analyzes two traits using DNMs from non-overlapping family samples. We first demonstrate that mTADA is able to leverage genetic overlaps to increase the statistical power of risk-gene identification. We then apply mTADA to large datasets of >13,000 trios for five neuropsychiatric disorders and CHD. We report additional risk genes for schizophrenia, epileptic encephalopathies and CHD. We outline some shared and specific biological information of intellectual disability and CHD by conducting systems biology analyses of genes prioritized by mTADA.
Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria
Authors: Kiga Kotaro , Tan Xin-Ee , Ibarra-Chávez Rodrigo , Watanabe Shinya , Aiba Yoshifumi , Sato’o Yusuke , Li Feng-Yu , Sasahara Teppei , Cui Bintao , Kawauchi Moriyuki , Boonsiri Tanit , Thitiananpakorn Kanate , Taki Yusuke , Azam Aa Haeruman , Suzuki Masato , Penadés José R. , Cui Longzhu 
Published: June 2020

AbstractThe emergence of antimicrobial-resistant bacteria is an increasingly serious threat to global health, necessitating the development of innovative antimicrobials. Here we report the development of a series of CRISPR-Cas13a-based antibacterial nucleocapsids, termed CapsidCas13a(s), capable of sequence-specific killing of carbapenem-resistant Escherichia coli and methicillin-resistant Staphylococcus aureus by recognizing corresponding antimicrobial resistance genes. CapsidCas13a constructs are generated by packaging programmed CRISPR-Cas13a into a bacteriophage capsid to target antimicrobial resistance genes. Contrary to Cas9-based antimicrobials that lack bacterial killing capacity when the target genes are located on a plasmid, the CapsidCas13a(s) exhibit strong bacterial killing activities upon recognizing target genes regardless of their location. Moreover, we also demonstrate that the CapsidCas13a(s) can be applied to detect bacterial genes through gene-specific depletion of bacteria without employing nucleic acid manipulation and optical visualization devices. Our data underscore the potential of CapsidCas13a(s) as both therapeutic agents against antimicrobial-resistant bacteria and nonchemical agents for detection of bacterial genes.
The halogen bond with isocyano carbon reduces isocyanide odor
Authors: Mikherdov Alexander S. , Novikov Alexander S. , Boyarskiy Vadim P. , Kukushkin Vadim Yu. 
Published: June 2020

AbstractPredominantly, carbon atoms of various species function as acceptors of noncovalent interactions when they are part of a π-system. Here, we report on the discovery of a halogen bond involving the isocyano carbon lone pair. The co-crystallization or mechanochemical liquid-assisted grinding of model mesityl isocyanide with four iodoperfluorobenezenes leads to a series of halogen-bonded adducts with isocyanides. The obtained adducts were characterized by single-crystal and powder X-ray diffraction, solid-state IR and 13C NMR spectroscopies, and also by thermogravimetric analysis. The formation of the halogen bond with the isocyano group leads to a strong reduction of the isocyanide odor (3- to 46-fold gas phase concentration decrease). This manipulation makes isocyanides more suitable for laboratory storage and usage while preserving their reactivity, which is found to be similar between the adducts and the parent isocyanide in some common transformations, such as ligation to metal centers and the multi-component Ugi reaction.
Eosinophil function in adipose tissue is regulated by Krüppel-like factor 3 (KLF3)
Authors: Knights Alexander J. , Vohralik Emily J. , Houweling Peter J. , Stout Elizabeth S. , Norton Laura J. , Alexopoulos Stephanie J. , Yik Jinfen. J. , Mat Jusoh Hanapi , Olzomer Ellen M. , Bell-Anderson Kim S. , North Kathryn N. , Hoehn Kyle L. , Crossley Merlin , Quinlan Kate G. R. 
Published: June 2020

AbstractThe conversion of white adipocytes to thermogenic beige adipocytes represents a potential mechanism to treat obesity and related metabolic disorders. However, the mechanisms involved in converting white to beige adipose tissue remain incompletely understood. Here we show profound beiging in a genetic mouse model lacking the transcriptional repressor Krüppel-like factor 3 (KLF3). Bone marrow transplants from these animals confer the beige phenotype on wild type recipients. Analysis of the cellular and molecular changes reveal an accumulation of eosinophils in adipose tissue. We examine the transcriptomic profile of adipose-resident eosinophils and posit that KLF3 regulates adipose tissue function via transcriptional control of secreted molecules linked to beiging. Furthermore, we provide evidence that eosinophils may directly act on adipocytes to drive beiging and highlight the critical role of these little-understood immune cells in thermogenesis.