bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human IDH mutant 1p/19q co-deleted gliomas have low tumor acidity as evidenced by molecular MRI and PET: a retrospective study
Authors: Yao Jingwen , Hagiwara Akifumi , Raymond Catalina , Shabani Soroush , Pope Whitney B. , Salamon Noriko , Lai Albert , Ji Matthew , Nghiemphu Phioanh L. , Liau Linda M. , Cloughesy Timothy F. , Ellingson Benjamin M. 
Published: July 2020

AbstractCo-deletion of 1p/19q is a hallmark of oligodendroglioma and predicts better survival. However, little is understood about its metabolic characteristics. In this study, we aimed to explore the extracellular acidity of WHO grade II and III gliomas associated with 1p/19q co-deletion. We included 76 glioma patients who received amine chemical exchange saturation transfer (CEST) imaging at 3 T. Magnetic transfer ratio asymmetry (MTRasym) at 3.0 ppm was used as the pH-sensitive CEST biomarker, with higher MTRasym indicating lower pH. To control for the confounder factors, T2 relaxometry and l-6-18F-fluoro-3,4-dihydroxyphenylalnine (18F-FDOPA) PET data were collected in a subset of patients. We found a significantly lower MTRasym in 1p/19q co-deleted gliomas (co-deleted, 1.17% ± 0.32%; non-co-deleted, 1.72% ± 0.41%, P = 1.13 × 10−7), while FDOPA (P = 0.92) and T2 (P = 0.61) were not significantly affected. Receiver operating characteristic analysis confirmed that MTRasym could discriminate co-deletion status with an area under the curve of 0.85. In analysis of covariance, 1p/19q co-deletion status was the only significant contributor to the variability in MTRasym when controlling for age and FDOPA (P = 2.91 × 10−3) or T2 (P = 8.03 × 10−6). In conclusion, 1p/19q co-deleted gliomas were less acidic, which may be related to better prognosis. Amine CEST-MRI may serve as a non-invasive biomarker for identifying 1p/19q co-deletion status.
Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area
Authors: Subramanian Kumaran , Sadaiappan Balamurugan , Aruni Wilson , Kumarappan Alagappan , Thirunavukarasu Rajasekar , Srinivasan Guru Prasad , Bharathi Selvaraj , Nainangu Prasannabalaji , Renuga Pugazhvendan Sampath , Elamaran Anandajothi , Balaraman Deivasigamani , Subramanian Mahendran 
Published: July 2020

AbstractMarine pollution is a significant issue in recent decades, with the increase in industries and their waste harming the environment and ecosystems. Notably, the rise in shellfish industries contributes to tons of shellfish waste composed of up to 58% chitin. Chitin, the second most ample polymer next to cellulose, is insoluble and resistant to degradation. It requires chemical-based treatment or enzymatic hydrolysis to cleave the chitin polymers. The chemical-based treatment can lead to environmental pollution, so to solve this problem, enzymatic hydrolysis is the best option. Moreover, the resulting biopolymer by-products can be used to boost the fish immune system and also as drug delivery agents. Many marine microbial strains have chitinase producing ability. Nevertheless, we still lack an economical and highly stable chitinase enzyme for use in the industrial sector. So we isolate a novel marine bacterial strain Achromobacter xylosoxidans from the shrimp waste disposal site using chitin minimal medium. Placket–Burman and central composite design statistical models for culture condition optimisation predicted a 464.2 U/ml of chitinase production. The culture conditions were optimised for maximum chitinase production recording up to 467 U/ml. This chitinase from the A. xylosoxidans was 100% active at an optimum temperature of 45 °C (withstand up to 55 °C) and pH 8 with 80% stability. The HPLC analysis of chitinase degraded shellfish waste reveals a major amino acid profile composition—arginine, lysine, aspartic acid, alanine, threonine and low levels of isoleucine and methionine. These chitinase degraded products and by-products can be used as supplements in the aquaculture industry.
Classification of α-synuclein-induced changes in the AAV α-synuclein rat model of Parkinson’s disease using electrophysiological measurements of visual processing
Authors: Østergaard Freja Gam , Himmelberg Marc M. , Laursen Bettina , Siebner Hartwig R. , Wade Alex R. , Christensen Kenneth Vielsted 
Published: July 2020

AbstractBiomarkers suitable for early diagnosis and monitoring disease progression are the cornerstone of developing disease-modifying treatments for neurodegenerative diseases such as Parkinson’s disease (PD). Besides motor complications, PD is also characterized by deficits in visual processing. Here, we investigate how virally-mediated overexpression of α-synuclein in thesubstantia nigra pars compactaimpacts visual processing in a well-established rodent model of PD. After a unilateral injection of vector, human α-synuclein was detected in the striatum and superior colliculus (SC). In parallel, there was a significant delay in the latency of the transient VEPs from the affected side of the SC in late stages of the disease. Inhibition of leucine-rich repeat kinase using PFE360 failed to rescue the VEP delay and insteadincreasedthe latency of the VEP waveform. A support vector machine classifier accurately classified rats according to their `disease state’ using frequency-domain data from steady-state visual evoked potentials (SSVEP). Overall, these findings indicate that the latency of the rodent VEP is sensitive to changes mediated by the increased expression of α-synuclein and especially when full overexpression is obtained, whereas the SSVEP facilitated detection of α-synuclein across reflects all stages of PD model progression.
Dopamine induces in vitro migration of synovial fibroblast from patients with rheumatoid arthritis
Authors: van Nie Lina , Salinas-Tejedor Laura , Dychus Nicole , Fasbender Frank , Hülser Marie-Lisa , Cutolo Maurizio , Rehart Stefan , Neumann Elena , Müller-Ladner Ulf , Capellino Silvia 
Published: July 2020

AbstractPreventing synovial fibroblast (SF) migration into the adjacent cartilage is a desirable therapeutic target in rheumatoid arthritis (RA). As previous studies demonstrated that RASF and SF from osteoarthritis (OA) patients express dopamine receptors (DR), aim of the present study was to investigate the impact of dopamine on mobility of fibroblasts from patients with chronic arthritides. Synovial tissue and fibroblasts were obtained from RA and OA patients. Immunohistochemistry was performed for all DR-subtypes in the invasion zone. Migration- and motility-assays were performed under DR-stimulation. Cytokines were evaluated using ELISA. Expression of DRs was evaluated by flow cytometry, and DR activation was measured by xCELLigence real-time analysis.
All DRs were expressed in RA invasion zone. Migration and motility of RASF and OASF were increased after DR stimulation in patients ≤ 75 years old. Synovial fibroblasts from older RA patients (> 75 years old) expressed lower levels of D1-, D2- and D4-DR than patients ≤ 75 years old. DR activation was not altered in older patients. Our results suggest a possible involvement of dopamine on migration of fibroblasts from arthritis patients. Therefore, the synovial dopaminergic pathway might represent a potential therapeutic target to interfere with progressive joint damage in RA patients.
Decreasing undesirable absorbed radiation to the intestine after administration of radium-223 dichloride for treatment of bone metastases
Authors: Ogawa Kazuma , Higashi Takuma , Mishiro Kenji , Wakabayashi Hiroshi , Shiba Kazuhiro , Odani Akira , Kinuya Seigo 
Published: July 2020

Abstract[223Ra]RaCl2 is the first alpha-particle emitting radiopharmaceutical to be used for castration-resistant prostate cancer patients with bone metastases because of its excellent therapeutic effects. [223Ra]RaCl2 is excreted via the intestine into feces, and some is absorbed from the intestine into the blood, which may be undesirable in terms of the exposure to radiation. Recently, we showed that a complex of myo-inositol-hexakisphosphate (InsP6) with zinc is a useful decorporation agent against radiostrontium. In this study, we hypothesized that Zn-InsP6 could bind to not only strontium but also to radium, and could inhibit the absorption of radium from the intestine. In in vitro binding experiments, Zn-InsP6 showed a high binding affinity for radium. In in vivo biodistribution experiments by intravenous injection of [223Ra]RaCl2 after treatment of Zn-InsP6, mice treated with Zn-InsP6 showed significantly lower bone accumulation of radioactivity (34.82 ± 1.83%Dose/g) than the mice in the non-treatment control group (40.30 ± 2.78%Dose/g) at 48 h postinjection. These results indicate that Zn-InsP6 bound radium in the intestine and inhibited the absorption of radium into the blood. Therefore, the insoluble Zn-InsP6 complex has high potential to decrease the side effects of [223Ra]RaCl2.
Vision-based reconstruction of laser projection with invariant composed of points and circle on 2D reference
Authors: Xu Guan , Chen Fang , Chen Rong , Li Xiaotao 
Published: July 2020

AbstractA vision-based reconstruction method is conducted by the point-circle invariant and the planar laser. The planar laser is coplanar to the two-dimensional (2D) reference. The combination of a circle on the 2D reference, a point on the 2D reference and a random point on the laser stripe is considered as the invariant, which is impervious to the projection from the laser plane to the image. Therefore, the reconstruction model is achieved by the invariant, which is generated from the projections of the above geometric features. The experiments are performed to verify the performance and reconstruction error of the method. The minimum error is 0.473 mm for the camera-reference distance of 600 mm, the scaleplate-projector distance of 100 mm and the reference length of 30 mm. Besides, the maximum error is 4.960 mm, for the camera-reference distance of 900 mm, the scaleplate-projector distance of 400 mm and the reference length of 120 mm. The reconstruction error means of 0.891 mm, 1.365 mm, 2.578 mm and 3.767 mm are observed with respect to the test conditions of 600–900 mm, which proves the applicability of the reconstruction method with the point-circle invariant.
P-dipping of rice seedlings increases applied P use efficiency in high P-fixing soils
Authors: Oo Aung Zaw , Tsujimoto Yasuhiro , Rakotoarisoa Njato Mickaël , Kawamura Kensuke , Nishigaki Tomohiro 
Published: July 2020

AbstractApplied phosphorus (P) use efficiency is generally low due to the low mobility of P in soil and its affinity to form insoluble complexes. Localized P application nearby the root zone is a potential approach to overcome this issue in crop production. However, the interaction with soil conditions is little understood, which results in less effective application of this approach. Using root-box experiments and changing P-retention capacity of soils, we revealed that applied P use efficiency of rice can be substantially improved by dipping seedlings in P-enriched slurry at transplanting (P-dipping) even in highly P-fixing soils. Spatial analysis of soluble P in soils indicated that P-dipping creates a P hotspot because the P-enriched slurry is transferred with seedling roots. The P hotspot could have induced vigorous surface root and facilitated further P uptake from the spot. In contrast, the effect of conventional P incorporation depended on P-retention capacity of soils; no increases in soluble P content in soils or plant P uptakes were observed when P-retention capacity was high. Our finding of significant interaction between localized P application and a specific soil property should help improving applied P use efficiency and achieving sustainable rice production against depleting P fertilizer resources.
Exploring the potential of the recombinant human collagens for biomedical and clinical applications: a short review
Authors: Chen Zhuoyue , Fan Daidi , Shang Lijun 
Published: July 2020

Abstract
Natural animal collagen and its recombinant collagen are favourable replacements in human tissue engineering due to their remarkable biomedical property. However, this exploitation is largely restricted due to the potential of immunogenicity and virus contamination. Exploring new ways to produce human collagen is fundamental to its biomedical and clinical application. All human fibrillar collagen molecules have three polypeptide chains constructed from a repeating Gly-Xaa-Yaa triplet, where Xaa and Yaa represent one random amino acid. Using cDNA techniques to modify several repeat sequences of the cDNA fragment, a novel human collagen, named recombinant human-like collagen (rHLC), with low immunogenicity and little risk from hidden virus can be engineered and notably tailored to speciﬁc applications. Human-like collagen (HLC) was initially used as a coating to modify the tissue engineering scaffold, and then used as the scaffold after cross-link agents were added to increase its mechanical strength. Due to its good biocompatibility, low immunogenicity, stabilised property, and the ability of mass production, HLC has been widely used in skin injury treatments, vascular scaffolds engineering, cartilage, bone defect repair, skincare, haemostatic sponge, and drug delivery, including coating with medical nanoparticles. In this review, we symmetrically reviewed the development, recent advances in design and application of HLC, and other recombinant human collagen-based biomedicine potentials. At the end, future improvements are also discussed.
Investigating the potential use of polymeric materials in the preparation of temporary cosmetic teeth painting veneers
Authors: Nazha Hasan 
Published: July 2020

AbstractDental whitening techniques and materials have become one of the most important non-surgical methods used to improve the appearance of teeth and to make them whiter and brighter, as well as to hide blemishes of the teeth. Since the veneer do not last forever and may fail due to fracture or adhesion failure or wrong selection of the appropriate design, removing large thickness of the outer layer of the tooth may make it vulnerable to weakness and long-term failure. This study aims to reach the polymeric combination that enables us to prepare temporary cosmetic teeth painting veneers, and to improve their properties, in order to get the veneers that work to maintain as much tooth structure as possible, hide defects and beautify teeth in the easiest ways and at least costs. The results showed that it is possible to prepare a paint mixture based on cheap and available raw materials consisting of sodium alginate as a polymeric matrix material in this mixture, titanium dioxide as a coloring additive, and calcium chloride solution as a bonding for sodium alginate molecules and improving for their properties. It is recommended to use the fourth combination (sodium alginate 2% + titanium dioxide 4%) immersed in a solution of calcium chloride (11.11% concentration) in the preparation of temporary paints due to its highest chemical stability and having appropriate physical and optical properties that enable them to use possibly as temporary cosmetic teeth painting veneers.
98 The Utility of Informant History in Delineating Narratives of Gait Disorder in Older Outpatients
Authors: Doyle P , Murphy C , Kennelly S , O'Neill D 
Published: July 2020

Abstract

Background
A scholarly focus on informant history is recent in geriatric medicine, mostly focused on cognition and syncope.1 Gait disorders are common in older people and accurate diagnosis facilitates treatment.2
As there is a disparity between self-rated and ‘objective’ estimates of health among older people, and gait disorders often co-exist with cognitive impairment, there may be a significant difference between patient and informant narratives of gait disorder. We investigated the existence and extent of this disparity.

Methods
As no suitable informant measure could be sourced from the biomedical literature, inquiry with senior academic physiotherapists or through appeals on social media relating to physiotherapy, a mobility scale was designed. We included all patients with a gait disorder at serial outpatient clinics. Exclusion criteria were absence of gait disorder and dementia of sufficient severity to be unable to participate in the consent process. Paired t tests and Fischer exact test were used to analyse the data.

Results
Of 45 participants (mean age 79.8; 50% men), 35 (77.7%) had MCI or dementia. Twenty two (47.8%) informants considered an acute event precipitated an acute deterioration in mobility. Twenty seven informants (60%) had a fear of the older person falling compared to 9 (20%) of patients (p=0.0002).
Patient and informant impression of point on mobility scale differed (t=3.61, p=0.0003). Although Informants reported more falls (mean of 1.36 vs 0.91) in the previous year (t=1.63, p=0.055) this did not achieve statistical significance.

Conclusions
Our findings demonstrate a discrepancy between patient and informant narratives of level of mobility, and point to the benefit of obtaining an informant history of loss of gait stability, mobility and falls. In addition, perceptions of fear of falling may be of assistance in managing risk and independence.

References
1. Briggs R, O'Neill D. QJM 2016; 109: 301–2.
2. Briggs R, O'Neill D. Clin Med 2014; 14: 200–2.
Control of Pickleworm on Squash with Selective Insecticides, 1997
Authors: Webb Susan E. 
Published: July 2020

Abstract
Insecticides specific for lepidopterous pest-were compared with a standard, Thiodan, and an untreated check for control of pickleworm. Squash was direct seeded on 1 Apr at the Central Florida Research and Education Center, Leesburg, FL. Each plot consisted of four 25-ft rows, 6 ft apart with plants spaced 2.5 ft apart. Treatments were replicated four times in an RCB design. All chemicals were applied with a 2-row tractor-mounted boom sprayer with 18 Albuz (lilac) ceramic hollow-cone nozzles spaced 8 inches apart, delivering 133 gpa at 200 psi. Latron CS-7, a surfactant, was tank-mixed with Confirm and RH-2485 at a concentration of 0.12% vol:vol. Treatments were applied weekly from 14 May to 11 Jun. Fruits were harvested, separated into marketable and damaged, counted, and weighed three times per week from 12 May to 16 Jun. An analysis of variance and a mean separation test (WD) were used to determine significant differences among treatments.
The Prevalence of Self-Reported Health Complaints and Health-Related Quality of Life in Women With Breast Implants
Authors: Miseré Renée M L , Colaris Maartje J L , Tervaert Jan W Cohen , van der Hulst René R W J 
Published: July 2020

Abstract

Background
Some of the millions of women with silicone breast implants (SBIs) report a pattern of systemic complaints, known as ASIA syndrome. However, the association between these complaints and breast implants remains uncertain.

Objectives
This study aimed to evaluate the prevalence of complaints in women with breast implants and healthy controls, and to compare their health-related quality of life.

Methods
Four groups of subjects were requested to fill in a general and a diagnostic questionnaire, and the Short Form 36. Group 1 was recruited from the Dutch foundation for breast implant illness (BII). Two groups were recruited from Dutch hospitals, where they had been augmented or reconstructed with SBIs (group 2) or saline-filled and hydrogel implants (group 3). A control group without breast implants was recruited from friends of subjects from group 2.

Results
In total, 238 women completed the questionnaires. ASIA manifestations appeared in the majority of the respondents (72.3%-98.8%), with a latency period of 0 to 35 years. Adjusted for age, smoking, and comorbidities, typical symptoms only occurred significantly more frequently in group 1. The presence of a chronic disease was an independent predictor for ASIA syndrome. The health-related quality of life was lower in women with SBIs than in women without breast implants.

Conclusions
The adjusted prevalence of BII manifestations is not significantly higher in women with SBIs than in women without implants. The findings of this study suggest that results on BII are subject to selection bias. Further studies are needed to prove an association between self-reported complaints and SBIs.

Level of Evidence: 2
Ecological Correlates of Rarity in a Tropical Forest Bird Community
Authors: Karr James R. 
Published: July 2020

Abstract
Many species were rare and a few species and individuals seasonal at a lowland seasonally wet forest site in central Panama. Rare species decreased in April-June, either because of nesting peaks or because food supplies are better (hence wandering less necessary) in these months. Of the rare species, many are large species with large home ranges and others wander from nearby second-growth or forest-edge habitats. Some are associated with a small stream adjacent to the study plot. Some follow army ants or have other specialized hunting techniques that may be correlated with their rarity. For a number of species rarity may also be correlated with limits to physiological capabilities and exclusion by local congeneric competitors, but definitive evidence for these patterns is hard to muster. Some species wander seasonally from wetter or dryer habitats in central Panama, suggesting some seasonal flux that keeps birds within forests of correct moisture (or other) characteristics at all times. The magnitude of these seasonal movements varies among wet and dry years. When the ecological correlates of rarity are compared for two Middle American forests, a number of similarities are found. Differences in the two areas are attributed to differences in sampling or to characteristics of the climate and/or nearby habitats.
Sexual Segregation of Red-Billed Queleas (Quelea quelea) in the Awash River Basin of Ethiopia
Authors: Jaeger M. M. , Erickson W. A. , Jaeger M. E. 
Published: July 2020

Abstract
Evidence is presented supporting a seasonal segregation by sex and age of the Red-billed Quelea (Quelea quelea) in and associated with the Awash River Basin of Ethiopia. Sexual segregation is most pronounced in the late dry season/early rains period (March-June), which precedes breeding (July-September). At this time the males appear to be more concentrated in the middle Awash Valley, in the general area where breeding will occur, while the females remain more dispersed. A balanced sex ratio is found from July to September. Following breeding there is a general dispersal with many queleas moving out to the cereal growing areas that border the Awash Valley. This process of dispersal results in a separation of adults from juveniles and, to a lesser degree, males from females.
Use of Space by Lapland Longspurs Breeding in Arctic Alaska
Authors: Tryon P. R. , MacLean S. F. 
Published: July 2020

Abstract
Use of space for foraging was compared with the defended area for three pairs of Lapland Longspurs breeding on arctic tundra near Barrow, Alaska. The activity space for foraging over the entire season was larger than the defended area and was considerably larger for males than for females. Throughout the season, greatest use was made of the area about the nest. This was more pronounced during the nestling and fledgling periods, when adults make frequent visits to feed the young. Greatest use was made of the defended area in the prenesting period, with a decline in use later in the season as territorial defense waned. Overlap occurred in the use of space by adjacent pairs. During the prenesting and incubation periods, this overlap compensated for the decline in use with distance from the nest, so that the combined use of space by adjacent birds was relatively even. Use of space is influenced by the time and energy requirements of breeding activities and by the availability of prey.