bluuebunny/crossref_metadata_2025_split huggingface dataset:
The application of laparoscopy combined with indocyanine green fluorescence imaging technique for hepatic cystic echinococcosis
Authors: Li Yupeng , Ma Zhigang , Tuxun Tuerhongjiang , Li Zhide , Meng Yuan , Chen Xiong 
Published: October 2020

Abstract
BackgroundWith the mature application of laparoscopy in hepatobiliary surgery, laparoscopic treatment of hepatic cystic echinococcosis(CE) has made certain progress. But, due to the inherent limitations of laparoscopy and the growth characteristics of cystic echinococcosis, distinguishing the boundary between cystic lesion and normal hepatic parenchyma is pivotal importance for successful sugery. Indocyanine green(ICG) fluorescence imaging technology can view the boundary of lesion and normal tissue during the treatment of hepatic cystic echinococcosis. Applied laparoscopy combined with ICG fluorescence imaging technique for hepatic cystic echinococcosis may be an effective surgical strategy.MethodsThe clinical data contained nine patients with hepatic cystic echinococcosis who underwent laparoscopic surgery with indocyanine green fluorescence imaging technique in authors’ institution from December 2018 to December 2019 were retrospectively analyzed. Indocyanine green was administered intravenously three days prior to surgery. The fluorescence acquisition system for real-time imaging was used during the surgery and the patients were followed up after surgery.ResultsOf reported nine patients, six are male and the remaining three are female. The average age is (36.4 ± 7.6) years. For all subjects, surgical procedures were performed under laparoscopy with indocyanine green fluorescence system. This technique showed the clear boundary of the hepatic cyst with normal liver paranchyma. Total cystectomy in six patients, subtotal cystectomy in two patients and partial hepatectomy in one patient were performed repectively. The average operation time was 3.8 ± 0.9 hours, blood loss 206.0 ± 120.7 ml. Neither blood transfusion nor post-operative complication was experienced. The average abdominal drainage time was 3.4 ± 0.9 days with hospital stay 5.7 ± 2.1 days. During the 6-12 months follow-up period, neither recurrence nor intraperitoneal implantation was found.ConclusionsApplied laparoscopy combined with ICG fluorescence imaging technique for hepatic cystic echinococcosis is safe and feasible. Enhanced boundary image can assist surgeons to complete radical resection and reduce complications.
Inhibitory effects of Camellia japonica&nbsp;on cell inflammation and acute rat reflux esophagitis
Authors: Nam Hyeon Hwa , Nan Li , Choo Byung Kil 
Published: October 2020

Abstract
Background: Oxidative stress and inflammation are the causes of many diseases. Reflux esophagitis (RE) is a common gastroesophageal reflux disease with a continuous upward trend in incidence worldwide. Camellia japonica (C. japonica) has long been used as a traditional herbal hemostatic medicine in China and Korea. The purpose of this study was to explore the anti-oxidant and anti-inflammatory activities of C. japonica and its protective effect against RE.Materials and methods: Buds from C. japonica plants were collected in a mountainous area of Jeju, South Korea. Dried C. japonica buds were extracted with 75% ethanol. DPPH and ABTS radical scavenging assays were performed. The ROS production and anti-inflammatory effects of C. japonica bud ethanol extract (CJE) on LPS-induced RAW264.7 cell inflammation were evaluated. The protective effects of CJE on RE were investigated in a RE rat model. Results: CJE eliminated over 50% of DPPH and ABTS radicals at concentrations of 100 and 200 μg/mL, respectively. CJE alleviated changes in cell morphology, reduced production of ROS, NO and IL-1β and down-regulated expression levels of iNOS, TNF-α, phosphorylated NF-κB, IκBα, and JNK/p38/MAPK. CJE reduced the esophageal tissue damage ratio (40.3%) and attenuation of histological changes. In addition, CJE down-regulated expression levels of TNF-α, IL-1β, and COX-2 and phosphorylation levels of NF-κB and IκBα in esophageal tissue. Conclusions: CJE possesses good anti-oxidation and anti-inflammatory activity, and can reduce RE damage in rats caused by gastric acid reflux. Therefore, CJE is a natural material that may be a candidate phytomedicine for the treatment of RE.
Immediate and legacy effects of snow exclusion on soil fungal diversity and community composition
Authors: Xu Zhenfeng , Zhang Li , Ren Yuzhi , Yang Kaijun , Li Zhijie , Tan Bo , Liu Yang , Li Han , You Chengming , Liu Sining , Wang Lixia , Yin Rui , Zhang Jian 
Published: October 2020

Abstract
Background: Soil fungi play crucial roles in ecosystem functions. However, how snow cover change associated with winter warming affects soil fungal communities remains unclear in the Tibetan forest.Methods: We conducted a snow manipulation experiment to explore immediate and legacy effects of snow exclusion on soil fungal community diversity and composition in a spruce forest on the eastern Tibetan Plateau. SoilThe fungal communities were performed by the high throughput sequencing of gene-fragments amplified from forest soil DNA.Results: We found that the Ascomycota and Basidiomycota were the two dominant fungal phyla and Archaeorhizomyces, Aspergillus and Amanita were the three most common genera across seasons and snow manipulations. Snow exclusion did not affect the diversity and structure of soil fungal community in both snow-covered and snow-free seasons. However, the relative abundance of some fungal communities was different among seasons. Soil fungal groups were correlated with environmental factors (i.e., temperature and moisture) and soil biochemical variables (i.e., ammonium and enzyme).Conclusions: These results suggest that the season-driven variations had stronger impacts on soil fungal community than short-term snow cover change. Such findings may have important implications for soil microbial processes in Tibetan forests experiencing significant decreases in snowfall.
A mixed-methods feasibility study of an arts-based intervention for patients receiving maintenance haemodialysis
Authors: Carswell Claire , Reid Joanne , Walsh Ian , Johnston William , McAneney Helen , Mullan Robert , Lee Jenny Baxley , Nelson Hugh , Matthews Michael , Weatherup Elizabeth , Spencer Andrea , Michelo Jean , Quail Anne , Kielty Grainne , Elliott Jenny , Mackenzie Alistair , Arbuckle Nicola , Wilson Anna , Noble Helen 
Published: October 2020

Abstract
BackgroundHaemodialysis can negatively impact quality of life and mental health. Arts-based interventions used successfully in other settings to improve health and well-being, could help address the impact of haemodialysis. This study aimed to evaluate the feasibility and acceptability of conducting a randomised controlled trial (RCT) of an arts-based intervention for patients receiving haemodialysisMethodsA parallel convergent mixed-methods design was used, including a pilot cluster RCT and qualitative process evaluation. Phase 1 evaluated recruitment and retention rates through a pilot cluster RCT at a single haemodialysis unit in Northern Ireland. Participants included patients who received haemodialysis for ESKD, were over the age of 18 and had the capacity to consent. These participants were randomised to the intervention or control group according to their haemodialysis shift. The intervention involved six one-hour, one-to-one facilitated arts sessions during haemodialysis. Phase 2 explored intervention and trial acceptability through a qualitative process evaluation using semi-structured interviews based on the RE-AIM framework. Participants included 13 patients who participated in phase 1 of the study, including 9 participants from the experimental group and four participants from the control group, and nine healthcare professionals who were present on the unit during implementation. Results:Out of 122 outpatient haemodialysis patients, 94 were assessed as eligible for participation. Twenty-four participants were randomised, meaning 80% of the target sample size was recruited and the attrition rate at 3 months was 12.5% (n=3). Participants viewed the arts as more accessible and enjoyable than anticipated following implementation. All participants who started the intervention (n=11) completed the full six sessions. Qualitative benefits of the intervention suggest improvements in mental well-being. Patient choice and facilitation were important factors for successful implementation. Conclusion:An arts-based intervention for patients receiving haemodialysis is acceptable for both patients and healthcare professionals, and a definitive trial is feasible. The intervention may help improve mental-wellbeing in patients receiving haemodialysis, but this requires further investigation in a definitive trial.Trial registrationThe trial was prospectively registered on clinicaltrials.gov on 14/8/2018, registration number NCT03629496.
Genome-wide association analysis reveals genetic variations and candidate genes associated with salt tolerance related traits in Gossypium hirsutum
Authors: Xu Peng , Guo Qi , Meng Shan , Zhang Xianggui , Xu Zhenzhen , Guo Wangzhen , Shen Xinlian 
Published: October 2020

Abstract
Background: Cotton is more resistant to salt and drought stresses as compared to other field crops, which makes itself as a pioneer industrial crop in saline-alkali lands. However, abiotic stresses still negatively affect its growth and development significantly. It is therefore important to breed salt tolerance varieties which can help accelerate the improvement of cotton production. The development of molecular markers linked to causal genes has provided an effective and efficient approach for improving salt tolerance. Results: In this study, a genome-wide association study (GWAS) of salt tolerance related traits at seedling stage was performed based on two years of phenotype identification for 217 representative upland cotton cultivars by genotyping-by-sequencing (GBS) platform. A total of 51,060 single nucleotide polymorphisms (SNPs) unevenly distributed among 26 chromosomes were screened across the cotton cultivars, and 25 associations with 27 SNPs scattered over 12 chromosomes were detected significantly (-log 10 p >4) associated with three salt tolerance related traits in 2016 and 2017. Among these, the associations on chromosome A13 and D08 for relative plant height (RPH), A07 for relative shoot fresh matter weight (RSFW), A08 and A13 for relative shoot dry matter weight (RSDW) were expressed in both environments, indicating that they were likely to be stable quantitative trait loci (QTLs). A total of 12 salt-induced candidate genes were identified differentially expressed by the combination of GWAS and transcriptome analysis. Three promising genes were selected for preliminary function verification of salt tolerance. The increase of GH_A13G0171-silenced plants in salt related traits under salt stress indicated its negative function in regulating the salt stress response. Conclusions: These results provided important genetic variations and candidate genes for accelerating the improvement of salt tolerance in cotton.
circBIRC6 regulates cisplatin resistance of choroidal melanoma via positively modulating ERK by sponging miR-503-3p
Authors: Li Wei , Nie Aiqin , Jin Longyu , Cui Yubo , Xie Ning 
Published: October 2020

Abstract
Background/AimscircRNA plays a key regulatory role in various human tumors. This study was carried out to investigate the molecular mechanism of circBIRC6 in the regulation of choroidal melanoma cisplatin (DDP) resistance. MethodsThe expression of circBIRC6 in choroidal melanoma tissues and cells (MUM-2B, MUM-2B / DDP) was detected by RT-qPCR. The function of circBIRC6 in DDP resistance of choroidal melanoma was analyzed by functional loss and overexpression experiments. DDP inhibition rate was detected by MTT method. Western blot was used to analysis the protein levels of P-gp, MRP1 and ERK. The relationship between circBIRC6, miR-503-3p and ERK was analyzed by CircRIP and luciferase reporter vector assay.ResultcircBIRC6 was up-regulated in choroidal melanoma, and it was significantly raised in choroidal melanoma tissues and cells that are resistant to DDP. CircBIRC6 regulated DDP resistance of choroidal melanoma cells by binding to miR-503-3p. ERK was directly targeted by miR-503-3p, and ERK inhibited miR-503-3p-induced DDP resistance. Finally, the expression of circBIRC6 was positively correlated with ERK, and it was verified that circBIRC6 regulated ERK by targeting miR-503-3p.ConclusionCircBIRC6 regulated the expression of ERK choroidal melanoma cells and DDP resistance via miR-503-3p. These results suggested that the knockout of circBIRC6 may provide an effective treatment strategy for choroidal melanoma.
The association of alcohol with circulating total fibrinogen and plasma clot density is mediated by fibrinogen and FXIII genotypes
Authors: Rautenbach Petro Hannie , Nienaber-Rousseau Cornelie , Pieters Marlien 
Published: October 2020

Abstract
Background: Alcohol consumption is associated with haemostasis and so may influence cardiovascular conditions. It is unknown whether the association of alcohol with total and g’ fibrinogen concentrations, as well as clot structure, are modulated by fibrinogen and factor (F)XIII single nucleotide polymorphisms (SNPs).Methods: Total fibrinogen, g’ fibrinogen and clot properties of 2010 healthy Africans residing in South Africa were measured in relation to alcohol intake as well as its markers – gamma-glutamyltransferase (GGT), percentage carbohydrate deficient transferrin (%CDT), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). Fourteen fibrinogen and two SNPs in the FXIII gene were genotyped to determine their influence.Results: Alcohol intake and its markers correlated negatively with fibrinogen and clot lysis time (CLT) as well as with most of the clot properties. Percentage g’ fibrinogen correlated positively with AST and negatively with alcohol intake. We then stratified for alcohol intake and found inverse associations between g’ fibrinogen and both %CDT and GGT–CDT in consumers, but the positive association with AST remained only in abstainers. Alcohol intake and its markers modulated the influence of fibrinogen SNPs on total fibrinogen concentrations and the fibrinogen SNPs as well as an FXIII SNP on clot density (all p <0.004).Conclusion/s: We show for the first time that some individuals harbour certain genotypes that, in combination with alcohol consumption, might predispose or protect them from haemostatic factors that might lead to the development of cardiovascular disease. Studies are needed to clarify the mechanisms related to the interplay between alcohol and gene variants observed here.
Collaborative Offloading for UAV-enabled Time-Sensitive MEC Networks
Authors: Li Wentao , Zhao Mingxiong , Wu Yuhui , Yu Junjie , Bao Lingyan , Yang Huan , Liu Di 
Published: October 2020

Abstract
Recently, unmanned aerial vehicle (UAV) acts as the aerial mobile edge computing (MEC) node to help the battery-limited Internet of Things (IoT) devices relieve burdens from computation and data collection, and prolong the lifetime of operating. However, IoT devices can ONLY ask UAV for either computing or caching help, and collaborative offloading services of UAV is rarely mentioned in the literature. Moreover, IoT device has multiple mutually independent tasks, which make collaborative offloading policy design even more challenging. Therefore, we investigate a UAV-enabled MEC networks with the consideration of multiple tasks either for computing or caching. Taking the quality of experience (QoE) requirement of time-sensitive tasks into consideration, we aim to minimize the total energy consumption of IoT devices by jointly optimizing trajectory, communication and computing resource allocation at UAV, and task offloading decision at IoT devices. Since this problem has highly non-convex objective function and constraints, we first decompose the original problem into three subproblems named as trajectory optimization ($\mathbf{P_T}$), resource allocation at UAV ($\mathbf{P_R}$) and offloading decisions at IoT devices ($\mathbf{P_O}$), then propose an iterative algorithm based on block coordinate descent method to cope with them in a sequence. Numerical results demonstrate that collaborative offloading can effectively reduce IoT devices’ energy consumption while meeting different kinds of offloading services, and satisfy the QoE requirement of time-sensitive tasks at IoT devices.
Selection of an Acceptable Method of Evaluating Nutritional Status in Hemodialysis Patients Compared to Subjective Global Assessment: A Cross-Sectional Study
Authors: Kohansal Atefe , Sohrabi Zahra , Mirzahosseini Hanieh , Naghibi Moein , Zare Morteza , Haghighat Neda , Akbarzadeh Marzie 
Published: October 2020

Abstract
Background: Protein-energy wasting (PEW) is prevalent among hemodialysis (HD) patients and is associated with poor outcomes. There are various methods for evaluating nutritional status in HD patients that each has its own advantages and disadvantages. We aimed at comparing the methods of normalized protein catabolic ratio (nPCR) and malnutrition universal screening tool (MUST) with subjective global assessment (SGA) in HD patients. Method: We examined 88 HD patients using SGA and MUST questionnaires, and also nPCR were calculated using predialysis and post-dialysis BUN, and Kt/v. Also, patients were assessed for PEW based on the Criteria of the International Society of Renal Nutrition and Metabolism. Methods’ specificity, sensitivity, and precision rates were assessed. Correlations between methods were analyzed using Pearson-correlation. Results: Based on the SGA, MUST, and nPCR methods, almost 41, 30, and 60 percent of patients had malnutrition, respectively. According to the criteria by International Society of Renal Nutrition and Metabolism, more than 90 percent of patients had PEW. SGA was positively and significantly associated with MUST (P≤0.001). Sensitivity for SGA, MUST, and nPCR methods were 100,100, 1.8 %, and their specificity were 98, 98, and 4 %, respectively and their precision rates were 99.7, 98.7, and 3%, respectively. Conclusion: From various methods of nutritional assessment (SGA, MUST, and nPCR), compared to SGA as the common method of nutrition assessment in hemodialysis patients, MUST had the nearest specificity, sensitivity, and precision rate compared to SGA and nPCR method had the lowest ones. nPCR seems to be a flawed marker of malnutrition and it should be more investigated whether MUST can be used instead of SGA or not.
Analysis of the Elders’ Intentions for Care and Its Influencing Factors Based on Andersen’s Behavioral Model
Authors: Chen Xuejiao , Lu Di , Tian Qingfeng , Shi Songhe 
Published: October 2020

Abstract
Background

Aging is a very pressing global situation, and with it comes the issue of care for the elderly. The objectives of this study were: ⑴ to understand the elders’ intention for care in Henan, China, and ⑵ to explore the association of intention based on Andersen’s Behavioral Model.
Methods

With multi-stage stratified cluster sampling, base on Andersen’s Behavioral Model, from the predisposing factors, enabling factor, and demand factor studying the influence factors of the elderly‘s intention.
Results

Home-based care is the main choice for the elderly, followed by institutional care, and finally community care. Enabling factors to have the greatest influence in this study. Factors influencing aged intention included: living status and total net monthly household income (enabling factors) and disability, life abilities objectively (need factors).
Conclusions

Home-based care for the aged is the main intention in Henan province. Marital status, living style, and income all have a relatively large impact on their old-age willingness. Old people with different physical conditions should provide different services so that they can have a comfortable life in old age.
A CVID-associated variant in the ciliogenesis protein CCDC28B disrupts immune synapse assembly
Authors: Capitani Nagaja , Onnis Anna , FINETTI FRANCESCA , Cassioli Chiara , Plebani Alessandro , Brunetti Jlenia , Troilo Arianna , D'Elios Sofia , Baronio Manuela , Gazzurrelli Luisa , Billadeau Daniel , D'Elios Mario , Lougaris Vassilios , Baldari Cosima 
Published: October 2020

Abstract
Ciliogenesis proteins orchestrate vesicular trafficking pathways that regulate immune synapse (IS) assembly in the non-ciliated T cells. We hypothesized that ciliogenesis-related genes might be disease candidates for common variable immunodeficiency with impaired T-cell function (T-CVID). We identified a heterozygous, predicted pathogenic variant in the ciliogenesis protein CCDC28B present with increased frequency in a large CVID cohort. We show that CCDC28B participates in IS assembly by regulating polarized T-cell antigen receptor (TCR) recycling. This involves the CCDC28B-dependent, FAM21-mediated recruitment of the actin regulator WASH to retromer at early endosomes to promote actin polymerization. The CVID-associated CCDC28BR25W variant failed to interact with FAM21, leading to impaired synaptic TCR recycling. CVID T cells carrying the ccdc28b C211T allele displayed IS defects mapping to this pathway that were corrected by overexpression of the wild-type allele. These results identify a new disease gene in T-CVID and pinpoint CCDC28B as a new player in IS assembly.
Circulating MiR-1290 as a Potential Diagnostic and Disease Monitoring Biomarker of Human Gastrointestinal Tumors
Authors: Xu Liyi , Chen Xiao , Zhu Yongliang , Cai Jianting 
Published: October 2020

Abstract
BackgroundGastrointestinal tumors are a leading cause of mortality worldwide. Our previous research demonstrated that miR-1290 is overexpressed in colorectal cancer (CRC) and promotes tumor progression. We therefore aimed to explore the potential of circulating miR-1290 as a biomarker for gastrointestinal cancer.MethodsSerum miRNA sequence analysis was performed. Then, circulating miRNA detection technologies were established. miR-1290 expression levels in gastrointestinal tumor cell lines and cellular supernatants were analyzed. Expression levels of circulating miR-1290 in clinical samples were examined. Associations between miR-1290 expression and clinicopathologic characteristics were analyzed. Xenograft models were conducted to assess the fluctuation of serum miR-1290 during disease progression.ResultsThrough miRNA sequencing, we identified that miR-1290 was overexpressed in CRC serum. We confirmed that human gastrointestinal tumor cells express and secret miR-1290. Circulating miR-1290 was up-regulated in pancreatic cancer (PC) (p < 0.01), CRC (p < 0.05), and gastric cancer (GC) (p < 0.01). High expression levels of miR-1290 were associated with tumor size, lymphatic invasion, vascular invasion, distant metastasis, tumor differentiation and AJCC stage in PC and CRC. The area under the curve (AUC) was 0.8857 in PC, with 60.9% sensitivity and 90.0% specificity. The AUC was 0.7852 in CRC, with 42.0% sensitivity and 90.0% specificity. In GC, the AUC was 0.6576, with 26.0% sensitivity and 90.0% specificity. The in vivo model verified that circulating miR-1290 levels significantly increased after tumor formation and decreased after drug treatment.ConclusionsOur findings demonstrate that circulating miR-1290 is a potential diagnostic and disease monitoring biomarker in gastrointestinal cancer.