bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of wild perennial Glycine species for resistance to soybean cyst nematode and soybean rust
Authors: Herman Theresa K. , Han Jaeyeong , Singh Ram J. , Domier Leslie L. , Hartman Glen L. 
Published: October 2020

AbstractThe genetic base for soybean cultivars is narrow compared to most other crop species. Twenty‐seven wild perennial Glycine species comprise the tertiary gene pool to soybean that may contain many genes of economic importance for soybean improvement. We evaluated 16 accessions of G. argyrea, G. clandestina, G. dolichocarpa, and G. tomentella for resistance to Heterodera glycines (HG), also known as the soybean cyst nematode, and to multiple isolates of Phakopsora pachyrhizi, the causal fungus of soybean rust. All 16 accessions were classified as resistant to H. glycines HG Type 2.5.7, based on number of cysts per root mass with plant introductions (PIs) 483227, 509501, 563892, and 573064 (all G. tomentella) void of any cysts indicating no reproduction by this pest. All 16 accessions had an immune reaction to one isolate of P. pachyrhizi. Regardless of isolate, no sporulating uredinia were observed on G. argyrea (PI 505151) and G. tomentella (PIs 483227, 509501, and 573064). These results demonstrate that some accessions within the perennial Glycine species harbour resistance to both H. glycines and P. pachyrhizi and would be good candidates for wide hybridization programs seeking to transfer potentially unique multiple resistance genes into soybean.
A HY5‐COL3‐COL13 regulatory chain for controlling hypocotyl elongation in Arabidopsis
Authors: Liu Bin , Long Hong , Yan Jing , Ye Lili , Zhang Qin , Chen Hongmei , Gao Sujuan , Wang Yaqin , Wang Xiaojing , Sun Shulan 
Published: October 2020

AbstractCONSTANS‐LIKE (COL) family members are commonly implicated in light signal transduction during early photomorphogenesis. However, some of their functions remain unclear. Here, we propose a role for COL13 in hypocotyl elongation in Arabidopsis thaliana. We found that COL13 RNA accumulates at high levels in hypocotyls and that a disruption in the COL13 function via a T‐DNA insertion or RNAi led to the formation of longer hypocotyls of Arabidopsis seedlings under red light. On the contrary, overexpression of COL13 resulted in the formation of shorter hypocotyls. Using various genetic, genomic, and biochemical assays, we proved that another COL protein, COL3, directly binds to the promoter of COL13, and the promoter region of COL3 was targeted by the transcription factor LONG HYPOCOTYL 5 (HY5), to form an HY5‐COL3‐COL13 regulatory chain for regulating hypocotyl elongation under red light. Additionally, further study demonstrated that COL13 interacts with COL3, and COL13 promotes the interaction between COL3 and CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), suggesting a possible COP1‐dependent COL3‐COL13 feedback pathway. Our results provide new information regarding the gene network in mediating hypocotyl elongation.
Nationwide survey of late‐onset hemolysis in very low birthweight infants
Authors: Miyazono Yayoi , Arai Junichi , Kanai Yu , Hitaka Daisuke , Kajikawa Daigo , Takeuchi Shusuke , Nagafuji Motomichi , Fujiyama Satoshi , Saito Makoto , Takada Hidetoshi 
Published: October 2020

AbstractBackgroundIn Japan, some cases of late‐onset acute hemolysis in very low birthweight (VLBW) infants have been reported. These cases had common features but the cause of hemolysis was unknown. The incidence and prognosis of this disease are also unknown. However, there are only few reports of such hemolytic episodes in countries other than Japan. Thus, this study aimed to examine the incidence and clinical course of late‐onset acute hemolysis and to establish it as a new disease concept.MethodsA nationwide prospective survey was conducted from 2011 to 2015 as a rare disease surveillance project of the Japan Society for Neonatal Health and Development.ResultsTwenty‐four cases were confirmed. The median (range) gestational age, birthweight, and onset of hemolytic episodes were 26 weeks and 2 days (23 weeks and 4 days–31 weeks and 2 days), 898 g (627–1,416 g), and 19 days after birth (9–33 days), respectively. Phototherapy, blood transfusion, and exchange transfusion were required in 22 (96%), 24 (100%), and 7 (29%) cases, respectively. During the observation period, no recurrence of the hemolytic episode occurred. All patients survived; however, one case developed kernicterus and suffered severe neurological sequelae.ConclusionsIn this study, at least 1 out of 1,259 VLBW infants developed hemolysis at 9–33 days after birth in Japan. Owing to the risk of kernicterus, this disease should be recognized as among the important pathological conditions of VLBW infants, suggesting the need to manage jaundice and anemia until 5 weeks after birth.
Sleep problems in children with autism spectrum disorder and typical development
Authors: Inthikoot Nipaporn , Chonchaiya Weerasak 
Published: October 2020

AbstractBackgroundAlthough higher sleep problems have been mostly reported in children with autism spectrum disorder (ASD) compared with typically developing (TD) children, particularly in Western countries, such evidence is relatively scarce in developing countries. We therefore investigated sleep difficulties in Thai children aged 3‐16 years with ASD compared with age‐ and gender‐matched TD children by using the Children's Sleep Habits Questionnaire (CSHQ)‐Thai version.MethodsSixty‐five children with ASD (mean age 97.7, SD 44.5 months; boys 70.8%) and 65 TD individuals (mean age 98.5, SD 43.5 months) were enrolled at a university‐based hospital in Bangkok. Background characteristics, sleep duration variables, and the CSHQ were completed by the participants' parents. The CSHQ subscales and total score between children with ASD and TD controls were then compared.ResultsChildren with ASD were more likely to have longer sleep latency than TD individuals for both weekdays and the weekend. Those with ASD had higher CSHQ subscales including bedtime resistance, sleep onset delay, sleep anxiety, and night waking in addition to the CSHQ total scores than TD controls. In the ASD group, those who took psychostimulants for treatment of ADHD had lower scores on the sleep duration subscale compared with unmedicated individuals.ConclusionsSleep difficulties were more prevalent in children with ASD compared with TD individuals. Parents should be advised to be aware of sleep problems in individuals with ASD. As such, sleep disturbances will be identified early, resulting in appropriate management and improved quality of life, not only for those with ASD but also their families.
Kidney transplantation in children with CAKUT and non‐CAKUT causes of chronic kidney disease: Do they have the same outcomes?
Authors: Monteverde Marta Lidia , Paz Marcos , Ibáñez Juan Pedro , Chaparro Alicia Beatriz , Solernou Veronica , Sager Cristian , Tessi Catalina , Locane Fabrizio 
Published: October 2020

AbstractAlmost half the children who undergo kidney transplantation (KTx) have congenital abnormalities of the kidney and urinary tract (CAKUT). We compared patient, graft survival, and kidney function at last follow‐up between CAKUT and non‐CAKUT patients after KTx. We divided the analysis into two eras: 1988‐2000 and 2001‐2019. Of 923 patients, 52% had CAKUT and 48% non‐CAKUT chronic kidney disease (CKD). Of the latter, 341 (77%) had glomerular disease, most frequently typical HUS (32%) and primary FSGS (27%); 102 had non‐glomerular disease. CAKUT patients were more often boys, younger at KTx, transplanted more frequently preemptively, but with longer time on chronic dialysis. They had less delayed graft function (DGF) and better eGFR, but higher incidence of urinary tract infection (1 year post‐KTx). In both eras, 1‐, 5‐, and 10‐year patient survival was similar in the groups, but graft survival was better in CAKUT recipients vs those with primary glomerular and primary recurrent glomerular disease: Era 1, 92.3%, 80.7%, and 63.6% vs 86.9%, 70.6%, and 49.5% (P = .02), and 76.7%, 56.6%, and 34% (P = .0003); Era 2, 96.2%, 88%, and 73.5% vs 90.3%, 76.1%, and 61% (P = .0075) and 75.4%, 54%, and 25.2% (P < .0001), respectively. Main predictors of graft loss were DGF, late acute rejection (AR), and age at KTx in CAKUT group and disease relapse, DGF, early AR, and number of HLA mismatches in recipients with glomerular disease. Graft survival was better in CAKUT patients. DGF was the main predictor of graft loss in all groups. Disease recurrence and early AR predicted graft failure in patients with glomerular disease.
Murder and Presidential Elections: A Cultivation‐Based Issue‐Ownership Theory of Local Television News and Its Geographic Structure
Authors: Ladewig Jeffrey W. 
Published: October 2020

AbstractThe theory that television, and in particular television violence, cultivates beliefs, attitudes, and actions in viewers, particularly heavy viewers, has long been a mainstay of media studies. Some critiques of the more recent empirical literature, however, argue that the current literature has lost some connection to the original theoretical formulations. This study attempts to answer some of these critiques by reengaging with the original conceptualization of cultivation analyses (i.e., institutional processes, message systems, and cultivation) as well as reaching out to find broader societal and political effects. Specifically, I argue and demonstrate that the rate of murder as funneled through the local television news cultivates voters in specific media markets, increasingly so in markets of resonance, toward a greater probability of voting for Republican presidential candidates.
Do shorter inter‐stimulus intervals in the go/no‐go task enable better assessment of response inhibition?
Authors: Hasegawa Akira , Matsumoto Noboru , Yamashita Yuko , Tanaka Keisuke , Kawaguchi Jun , Yamamoto Tetsuya 
Published: October 2020

Young, Sutherland, and McCoy indicated that a Go/No‐Go Task (GNG) becomes more difficult as the inter‐stimulus intervals (ISIs) becomes shorter. However, is the number of commission errors under extremely short ISIs a useful metric for assessing response inhibition? This study challenges the assumption that a shorter ISI in the GNG enables better assessment of response inhibition. University students (N = 213) completed the GNG, the Conners Continuous Performance Test 3rd Edition (CCPT), and the Modified Stroop Task. The GNG comprised four blocks of 400, 600, 800, and 1000 ms ISIs, whereas the stimulus presentation was fixed at 250 ms. Consistent with Young et al., shorter ISIs in the GNG resulted in more commission errors. In the block with the shortest ISI, participants also failed more frequently in responses in go trials than in the other blocks, which appears to increase in error variance of commission errors. Consistent with this interpretation, the association between the number of commission errors in the block with 400 ms ISI and CCPT performance was weaker than those between the number of commission errors in other blocks and CCPT performance. It is concluded that using the number of commission errors in the condition with extremely short ISIs in the GNG might be inappropriate for assessing response inhibition.
A study of factors impacting elementary mathematics preservice teachers: Improving mindfulness, anxiety, self‐efficacy, and mindset
Authors: Tassell Janet , Gerstenschlager Natasha E. , Syzmanski Toni , Denning Savannah 
Published: October 2020

AbstractInnovation is more imperative now than ever before given the upcoming shortage in prepared teachers and the need to produce students with a strong knowledge of mathematics. A sense of urgency is impacting teacher education/preparation programs as instructional practices need to discover how to arm teachers to increase the number of students to be not only college‐ready but also desiring to pursue Science, Technology, Engineering, and Mathematics majors. As such, the purpose of this study, was to determine how the four variables (mindfulness, mathematics anxiety, self‐efficacy, and mindset) are interconnected within preservice elementary teachers (PSETs), and how we as teacher educators can better address these variables within our own PSETs. Each semester included three seminars with similar overall foci including the four variables. Participants in this study were recruited from Elementary Education students at an east south central regional university enrolled in a mathematics methods course. Thirty‐seven participants were divided into control (N = 20) and treatment (N = 17). In this article, we present both qualitative and quantitative results from our mixed‐methods study that considered these questions. With the results of this study revealing an inter‐connectedness among the four variables, this research further informs the teacher educator community.
Infiltrating Kaposi sarcoma presenting as acute kidney injury: An unexpected consequence of deliberate hepatitis C–positive organ transplantation
Authors: Story Maria T. , Sanders M. Lee , Bashir Amani A. , Longo Jude M. , Abel Stacy L. , Dollard Sheila C. , Grodstein Elliot I. , Thomas Christie P. , Katz Daniel A. 
Published: October 2020

AbstractKaposi sarcoma (KS) following kidney transplantation can result from recipient reactivation of latent human herpesvirus 8 (HHV‐8) infection or activation of donor‐acquired HHV‐8 infection. Post‐transplant KS typically manifests with cutaneous pathology, but rare cases of renal allograft involvement have been reported. We describe two cases of donor‐derived HHV‐8 infection in two hepatitis C (HCV) viremia‐negative transplant recipients who each received a kidney from a donor with HCV viremia. One recipient did not develop KS while the other presented with acute kidney injury caused by extensive KS infiltration of the renal parenchyma and metastatic disease. This report reviews the literature for cases of KS involving the renal allograft and highlights an unexpected consequence of deliberate HCV‐positive organ transplantation.
Accurate detection of Zika virus IgG using a novel immune complex binding ELISA
Authors: Ehmen C. , Medialdea‐Carrera R. , Brown D. , de Filippis A.M. Bispo , de Sequeira P.Carvalho , Nogueira R.M. Ribeiro , Brasil P. , Calvet G.A. , Blessmann J. , Mallmann A.‐M. , Sievertsen J. , Rackow A. , Schmidt‐Chanasit J. , Emmerich P. , Schmitz H. , Deschermeier C. , Mika A. 
Published: October 2020

AbstractObjectivesAccurate serological assays are urgently needed to support public health responses to Zika virus (ZIKV) infection with its potential to cause foetal damage during pregnancy. Current flavivirus serology for ZIKV infections lacks specificity due to cross‐reacting antibodies from closely related other flaviviruses. In this study, we evaluated novel serological tests for accurate ZIKV IgG detection.MethodsOur ELISAs are based on immune complex binding. The high specificity is achieved by the simultaneous incubation of labelled ZIKV antigen and unlabelled flavivirus homolog protein competitors. Two assays were validated with a panel of 406 human samples from PCR‐confirmed ZIKV patients collected in Brazil (n = 154), healthy blood donors and other infections from Brazil, Europe, Canada and Colombia (n = 252).ResultsThe highest specificity (100% [252/252, 95% confidence interval (CI) 98.5–100.0]) was shown by the ZIKV ED3 ICB ELISA using the ED3 antigen of the ZIKV envelope. A similar test using the NS1 antigen (ZIKV NS1 ICB ELISA) was slightly less specific (92.1% [232/252, 95% CI 88.0–95.1]). The commercial Euroimmun ZIKV ELISA had a specificity of only 82.1% (207/252, 95% CI 76.8–86.7). Sensitivity was high (93–100%) from day 12 after onset of symptoms in all three tests. Seroprevalence of ZIKV IgG was analysed in 87 samples from Laos (Asia) confirming that the ED3 ELISA showed specific reactions in other populations.ConclusionsThe novel ED3 ICB ELISA will be useful for ZIKV‐specific IgG detection for seroepidemiological studies and serological diagnosis for case management in travellers and in countries where other flavivirus infections are co‐circulating.
Detection of dengue, chikungunya, and Zika RNA in blood donors from Southeast Asia
Authors: Stanley Jean , Chongkolwatana Viroje , Duong Pham Tuan , Kitpoka Pimpun , Stramer Susan L. , Dung Nguyen Thi Thanh , Grimm Kacie E. , Pojanasingchod Anyarin , Suksomboonvong Panitita , Galel Susan A. 
Published: October 2020

AbstractBackgroundChikungunya (CHIKV), dengue (DENV), and Zika (ZIKV) viruses are of concern due to the potential of transfusion transmission in blood, especially in regions such as Southeast Asia where the viruses are endemic. The recent availability of nucleic acid testing (NAT) to screen blood donations on an automated platform provides the opportunity to detect potentially infectious units in asymptomatic donors.Study Design and MethodsThree thousand blood donations from Vietnam and 6000 from Thailand were screened with a real‐time polymerase chain reaction (PCR) test (cobas CHIKV/DENV, Roche Diagnostics, Indianapolis, IN) and equal numbers on cobas Zika (Roche Diagnostics). Reactive samples were tested by alternative NAT with resolution of discordant results by heminested PCR. Throughput of simultaneous testing of the two assays on the cobas 8800 system (Roche Diagnostics) was evaluated.ResultsIn Vietnam, 9 of 3045 samples were reactive for DENV and all were confirmed, for a prevalence (with 95% confidence interval [CI]) of 0.296% (0.135‐0.560). In Thailand, 2 of 6000 samples were reactive for CHIKV, 4 of 6000 for DENV, and 1 of 6005 for ZIKV, and all confirmed. The prevalence of CHIKV is 0.033% (0.004‐0.120), DENV 0.067% (0.018‐0.171), and ZIKV 0.017% (0.000‐0.093). The overall specificity for the cobas CHIKV/DENV and cobas Zika tests was 100% (99.959‐100).For the simultaneous assay testing, 960 test results were available in 7 hours and 53 minutes.ConclusionDetection of CHIKV, DENV, and ZIKV RNA in donor samples in Vietnam and Thailand indicate the presence of the virus in asymptomatic blood donors. The cobas 6800/8800 systems (Roche Molecular Systems, Pleasanton, CA) enable screening blood donations in endemic areas for these viruses together or separately.
Constructing Rigid-Foldable Generalized Miura-Ori Tessellations for Curved Surfaces
Authors: Hu Yucai , Zhou Yexin , Liang Haiyi 
Published: October 2020

Abstract
Origami has shown the potential to approximate three-dimensional curved surfaces by folding through designed crease patterns on flat materials. The Miura-ori tessellation is a widely used pattern in engineering and tiles the plane when partially folded. Based on constrained optimization, this article presents the construction of generalized Miura-ori patterns that can approximate three-dimensional parametric surfaces of varying curvatures while preserving the inherent properties of the standard Miura-ori, including developability, flat foldability, and rigid foldability. An initial configuration is constructed by tiling the target surface with triangulated Miura-like unit cells and used as the initial guess for the optimization. For approximation of a single target surface, a portion of the vertexes on the one side is attached to the target surface; for fitting of two target surfaces, a portion of vertexes on the other side is also attached to the second target surface. The parametric coordinates are adopted as the unknown variables for the vertexes on the target surfaces, while the Cartesian coordinates are the unknowns for the other vertexes. The constructed generalized Miura-ori tessellations can be rigidly folded from the flat state to the target state with a single degree-of-freedom.
Epidemiological surveillance study of female genital mutilation in the UK
Authors: Hodes Deborah , Ayadi O’Donnell Najette , Pall Karina , Leoni Marina , Lok Wingsan , Debelle Geoff , Armitage Alice Jane , Creighton Sarah M , Lynn Richard M 
Published: October 2020

Objectives
Describe cases of female genital mutilation (FGM) presenting to consultant paediatricians and sexual assault referral centres (SARCs), including demographics, medical symptoms, examination findings and outcome.

Design
The well-established epidemiological surveillance study performed through the British Paediatric Surveillance Unit included FGM on the monthly returns.

Setting
All consultant paediatricians and relevant SARC leads across the UK and Ireland.

Patients
Under 16 years old with FGM.

Interventions
Data on cases from November 2015 to November 2017 and 12 months later meeting the case definition of FGM.

Main outcome measures
Returns included 146 cases, 103 (71%) had confirmed FGM and 43 (29%) did not meet the case definition. There were none from Northern Ireland.

Results
The mean reported age was 3 years. Using the WHO classification of FGM, 58% (n=60) had either type 1 or type 2, 8% (n=8) had type 3 and 21% (n=22) had type 4. 13% (n=13) of the cases were not classified and none had piercings or labiaplasty. The majority, 70% had FGM performed in Africa with others from Europe, Middle East and South-East Asia. There were few physical and mental health symptoms. Only one case resulted in a successful prosecution.

Conclusions
There were low numbers of children presenting with FGM and in the 2 years there was only one prosecution. The findings may be consistent with attitude changes in FGM practising communities and those at risk should be protected and supported by culturally competent national policies
Multiregion whole-exome sequencing of intraductal papillary mucinous neoplasms reveals frequent somaticKLF4mutations predominantly in low-grade regions
Authors: Fujikura Kohei , Hosoda Waki , Felsenstein Matthäus , Song Qianqian , Reiter Johannes G , Zheng Lily , Beleva Guthrie Violeta , Rincon Natalia , Dal Molin Marco , Dudley Jonathan , Cohen Joshua D , Wang Pei , Fischer Catherine G , Braxton Alicia M , Noë Michaël , Jongepier Martine , Fernández-del Castillo Carlos , Mino-Kenudson Mari , Schmidt C Max , Yip-Schneider Michele T , Lawlor Rita T , Salvia Roberto , Roberts Nicholas J , Thompson Elizabeth D , Karchin Rachel , Lennon Anne Marie , Jiao Yuchen , Wood Laura D 
Published: October 2020

ObjectiveIntraductal papillary mucinous neoplasms (IPMNs) are non-invasive precursor lesions that can progress to invasive pancreatic cancer and are classified as low-grade or high-grade based on the morphology of the neoplastic epithelium. We aimed to compare genetic alterations in low-grade and high-grade regions of the same IPMN in order to identify molecular alterations underlying neoplastic progression.DesignWe performed multiregion whole exome sequencing on tissue samples from 17 IPMNs with both low-grade and high-grade dysplasia (76 IPMN regions, including 49 from low-grade dysplasia and 27 from high-grade dysplasia). We reconstructed the phylogeny for each case, and we assessed mutations in a novel driver gene in an independent cohort of 63 IPMN cyst fluid samples.ResultsOur multiregion whole exome sequencing identifiedKLF4, a previously unreported genetic driver of IPMN tumorigenesis, with hotspot mutations in one of two codons identified in >50% of the analyzed IPMNs. Mutations inKLF4were significantly more prevalent in low-grade regions in our sequenced cases. Phylogenetic analyses of whole exome sequencing data demonstrated diverse patterns of IPMN initiation and progression. Hotspot mutations inKLF4were also identified in an independent cohort of IPMN cyst fluid samples, again with a significantly higher prevalence in low-grade IPMNs.ConclusionHotspot mutations inKLF4occur at high prevalence in IPMNs. Unique among pancreatic driver genes,KLF4mutations are enriched in low-grade IPMNs. These data highlight distinct molecular features of low-grade and high-grade dysplasia and suggest diverse pathways to high-grade dysplasia via the IPMN pathway.