bluuebunny/crossref_metadata_2025_split huggingface dataset:
On designing experiments for a dynamic response modeled by regression splines
Authors: Pan Rong , Saleh Moein 
Published: October 2019

AbstractDynamic response systems are often found in science, engineering, and medical applications, but the discussion on experimental design for such a system is relatively rare in literature. For an experimenter, designing such experiments requires making decisions on (1) when or where to take response measurements along the dynamic variable and (2) how to choose the combination of experimental factors and their levels. The first consideration is unique for such experiments, especially when the measurement cost is high. In this paper, we present a design approach through the mixed‐effect linear model, which is based on a hierarchical B‐spline function for the dynamic response. We develop several theorems that can assist in finding a statistically efficient sampling plan and propose an algorithm for searching the D‐optimal design of a dynamic response system.
Uncovering the In Vivo Proxisome Using Proximity‐Tagging Methods
Authors: Santin Yoann G. 
Published: October 2019

AbstractThe development of new approaches is critical to gain further insights into biological processes that cannot be obtained by existing methods or technologies. The detection of protein–protein interaction is often challenging, especially for weak and transient interactions or for membrane proteins. Over the last decade, several proximity‐tagging methodologies have been developed to explore protein interactions in living cells. Among those, the most efficient are based on protein partner modification, such as biotinylation or pupylation. Such technologies are based on engineered variants of enzymes like peroxidases or ligases that release reactive molecules, in the presence of specific substrates, that bind surrounding proteins. Fusing a protein of interest (POI) to these enzymes allows the definition of an unbiased “proxisome,” that is, all of the proteins in interaction or in close vicinity of the POI. Here, the different proximity‐labeling tools available are described and comprehensive comparison to discuss advantages and limitations is provided.
Percutaneous radiofrequency-assisted liver partition versus portal vein embolization before hepatectomy for perihilar cholangiocarcinoma
Authors: Melekhina O. , Efanov M. , Alikhanov R. , Tsvirkun V. , Kulezneva Y. , Kazakov I. , Vankovich A. , Koroleva A. , Khatkov I. 
Published: October 2019

Abstract

Background
Percutaneous radiofrequency-assisted liver partition with portal vein embolization in staged liver resection (PRALPPS) represents an alternative to portal vein embolization (PVE) followed by major liver resection in patients with perihilar cholangiocarcinoma.

Methods
This was an observational case–control study. Both procedures were applied in patients with a future liver remnant (FLR) volume of less than 40 per cent. The main end points of the study were short-term morbidity and mortality for the two procedures. The study also compared the efficacy of the preresection phases estimated by kinetic growth rate (KGR), time interval and degree of hypertrophy of the FLR.

Results
The first phase (preresection) was completed in 11 and 18 patients, and the second phase (resection) in nine and 14 patients, in the PRALPPS and PVE groups respectively. Major morbidity after the first stage did not differ between the groups. There were no differences in blood loss, severe morbidity or liver failure rate after the second stage, with no deaths. The mean KGR of the FLR after the preresection phase for PRALPPS was 3·8 (0·6–9·8) per cent/day, and that after PVE was 1·8 (0–6·7) per cent/day (P = 0·037). The mean time interval for FLR hypertrophy in the PRALPPS and PVE groups was 15 (6–29) and 20 (8–35) days respectively (P = 0·039).

Conclusion
Short-term outcomes were similar for PRALPPS and PVE in terms of safety. Remnant hypertrophy was achieved more rapidly by PRALPPS.
Design, implementation, and outcomes of an elective course on preliminary structural biology for undergraduate students majoring in biotechnology
Authors: Zhang Jinyong , Jing Haiming , Luo Ping , Zhang Xiaoli , Zou Quanming 
Published: October 2019

AbstractBiotechnological pharmaceuticals is a key course offered to third‐year undergraduates majoring in biotechnology in our university. However, students often experience difficulties in understanding the principles of related technologies. In this study, we developed and implemented an elective course on preliminary structural biology for biotechnology undergraduates, aiming at reinforcing the principles of these technologies by experimental practice. The course was composed of three phases and lasted for 15 weeks, 18 students were randomly divided into six teams and were encouraged to design, prepare, carry out, and conclude a project on their own. The main contents of their project were cloning, expression, purification, and crystal screening of HpaA, a lipoprotein from the gastric pathogen Helicobacter pylori. Examination scores of biotechnology pharmaceuticals were used to assess learning outcomes. The results showed that students who participated in this course gained higher scores in the final examination, and they performed better on the questions specifically related to the elective course. These results demonstrated that the course enhanced students' understanding of the technologies involved in this course by practical applications. Thus, this elective course was effective in helping biotechnology undergraduates to learn the theory and application of biological technologies, and the experience gained in this course may be useful for other technology‐based courses.
Composition and Intraspecific Chemical Variability of Leaf Essential Oil of Laggera pterodonta from Côte d'Ivoire
Authors: Kambiré Didjour Albert , Brice Boti Jean , Yapi Thierry Acafou , Ouattara Zana Adama , Paoli Mathieu , Bighelli Ange , Tomi Félix , Casanova Joseph 
Published: October 2019

AbstractThe chemical composition of 44 leaf oil samples of Laggera pterodonta (DC.) Sch.Bip. ex Oliv. (Asteraceae) from Côte d'Ivoire was investigated, using combination of chromatographic (GC‐FID) and spectroscopic (GC/MS, 13C‐NMR) techniques. Two oil samples chosen according to their chromatographic profiles were submitted to column chromatography and all fractions of CC were analyzed by GC‐FID, GC/MS and 13C‐NMR. In total, 83 components accounting for 96.5 to 99.4 % of the whole chemical composition were identified. Significant variations were observed within terpene classes: monoterpene hydrocarbons (0.4–22.7 %), oxygenated monoterpenes (32.9–54.9 %), sesquiterpene hydrocarbons (18.6–38.3 %) and oxygenated sesquiterpenes (3.5–38.4 %). Thus, the 44 compositions were subjected to hierarchical cluster analysis (HCA) and principal component analysis (PCA). Two groups were differentiated according to their composition. All the samples contained 2,5‐dimethoxy‐p‐cymene, α‐humulene and (E)‐β‐caryophyllene among the main components. Other components were present at appreciable contents and allowed differentiation of two groups: sabinene and germacrene D for Group I; 10‐epi‐γ‐eudesmol and eudesm‐7(11)‐en‐4α‐ol for Group II. All the samples collected in Eastern Côte d'Ivoire constituted Group I, while samples collected in the Central area of the country constituted Group II.
Carbon materials from melamine sponges for supercapacitors and lithium battery electrode materials: A review
Authors: Shi Yanying , Liu Guijing , Jin Rencheng , Xu Hui , Wang Qingyao , Gao Shanmin 
Published: October 2019

AbstractWith the increasing energy demand together with the deteriorating environment and decreasing fossil fuel resources, the development of highly efficient energy conversion and storage devices is one of the key challenges of both fundamental and applied research in energy technology. Melamine sponges (MS) with low density, high nitrogen content, and high porosity have been used to design and obtain three‐dimensional porous carbon electrode materials. More importantly, they are inexpensive, environment‐friendly, and easy to synthesize. There have been many reports on the modification of carbonized MS and MS‐based composites for supercapacitor and lithium battery electrode materials. In this paper, recent studies on the fabrication of electrode materials using MS as raw materials have been mainly reviewed, including carbonation, doping activation, and composite modification of MS, and expectations for the development of porous carbon materials for energy storage as a reference with excellent performance, environment‐friendliness, and long life.
Mitochondria‐Targeting Selenophene‐Modified BODIPY‐Based Photosensitizers for the Treatment of Hypoxic Cancer Cells
Authors: Karaman Osman , Almammadov Toghrul , Emre Gedik M. , Gunaydin Gurcan , Kolemen Safacan , Gunbas Gorkem 
Published: October 2019

AbstractTwo red‐absorbing, water‐soluble and mitochondria (MT)‐targeting selenophene‐substituted BODIPY‐based photosensitizers (PSs) were realized (BOD−Se, BOD−Se−I), and their potential as photodynamic therapy (PDT) agents were evaluated. BOD−Se−I showed higher 1O2 generation yield thanks to the enhanced heavy‐atom effect, and this derivative was further tested in detail in cell culture studies under both normoxic and hypoxic conditions. BOD−Se−I not only effectively functioned under hypoxic conditions, but also showed highly selective photocytotoxicity towards cancer cells. The selectivity is believed to arise from differences in mitochondrial membrane potentials of healthy and cancerous cells. To the best of our knowledge, this marks the first example of a MT‐targeted BODIPY PS that functions under hypoxic conditions. Remarkably, thanks to the design strategy, all these properties where realized by a compound that was synthesized in only five steps with 32 % overall yield. Hence, this material holds great promise for the realization of next‐generation PDT drugs for the treatment of hypoxic solid tumors.
Discovery and Pharmacophore Mapping of a Low‐Nanomolar Inhibitor of P. falciparum Growth
Authors: Bassanini Ivan , Parapini Silvia , Galli Corinna , Vaiana Nadia , Pancotti Andrea , Basilico Nicoletta , Taramelli Donatella , Romeo Sergio 
Published: October 2019

AbstractThe treatment of malaria, the most common parasitic disease worldwide and the third deadliest infection after HIV and tuberculosis, is currently compromised by the dramatic increase and diffusion of drug resistance among the various species of Plasmodium, especially P. falciparum (Pf). In this view, the development of new antiplasmodial agents that are able to act via innovative mechanisms of action, is crucial to ensure efficacious antimalarial treatments. In one of our previous communications, we described a novel class of compounds endowed with high antiplasmodial activity, characterized by a pharmacophore never described before as antiplasmodial and identified by their 4,4’‐oxybisbenzoyl amide cores. Here, through a detailed structure‐activity relationship (SAR) study, we thoroughly investigated the chemical features of the reported scaffolds and successfully built a novel antiplasmodial agent active on both chloroquine (CQ)‐sensitive and CQ‐resistant Pf strains in the low nanomolar range, without displaying cross‐resistance. Moreover, we conducted an in silico pharmacophore mapping.
Secure analysis on entire software‐defined network using coloring distribution model
Authors: Xin‐Hui Zhao , Ze‐Hui Wu , Xiao‐Bin Song , Qing‐Xian Wang 
Published: October 2019

SummarySoftware Definition Network (SDN) has three features as separation of control and forwarding, unified management of configuration, and dynamic programming, which have greatly improved flexibility of network deployment and dynamics of network management, as well as efficiency of network transmission. However, its security problem is quite outstanding. This paper proposes a new security defense method based on coloring distribution model, which aims at the shortcomings of the current research that does not change the weak security, certainty, statics, and isomorphism of SDN. Motivated by the idea of moving target defense, our method abstracts network topology of SDN using coloring theory and realizes diversified deployment of controllers and switches, thus improving the security of network itself without changing the structure of SDN. Simulation results show that our method can prevent denial of service (DOS) attacks against controllers and switches and at the same time effectively block the worm, which is one of the most threat of smart city, propagation via switches.
Glycine‐Induced Electrodeposition of Nanostructured Cobalt Hydroxide: A Bifunctional Catalyst for Overall Water Splitting
Authors: Shilpa Nagaraju , Nadeema Ayasha , Kurungot Sreekumar 
Published: October 2019

AbstractHerein, an interconnected α‐Co(OH)2 structure with a network‐like architecture was used as a bifunctional electrocatalyst for the overall water splitting reaction in alkaline medium. The complexing ability of glycine with a transition metal was exploited to form [Co(gly)3]− dispersion at pH 10, which was used for the electrodeposition. High‐resolution TEM, UV/Vis‐diffuse reflectance spectroscopy, and X‐ray photoelectron spectroscopy were used to confirm that the as‐synthesized materials had an α‐Co(OH)2 phase. The electrocatalytic oxygen and hydrogen evolution activity of the glycine‐coordinated α‐Co(OH)2 was found to be approximately 320 and 145 mV, respectively, at 10 mA cm−2. The material required approximately 1.60 V (vs. reversible hydrogen electrode; RHE) to achieve the benchmark of 10 mA cm−2 for overall water splitting with a mass activity of approximately 63.7 A g−1 at 1.60 V (vs. RHE). The chronoamperometric response was measured to evidence the stability of the material for overall water splitting for up to 24 h. Characterization of the catalyst after the oxygen and hydrogen evolution reactions was performed by XPS and showed the presence of a CoII/CoIII oxidation state.
Radio resource management framework for energy‐efficient communications in the Internet of Things
Authors: G. C. Deepak , Bouhafs Faycal , Raschellà Alessandro , Mackay Michael , Shi Qi 
Published: October 2019

AbstractThe Internet of Things (IoT) is the vision of a global network that connects various physical world objects to the IT infrastructure through a wireless medium. Despite the availability of a number of mature radio access technologies such as GSM, Long‐Term Evolution, Wi‐Fi, and due to the current progress made in developing 5G technology, more and more IoT operators are opting to use low‐power wide‐area (LPWA) technologies due to their low cost and easy deployment. However, recent studies show that the radio resource allocation used in these technologies is not scalable. This limitation often results in packet collisions, retransmission and unnecessary waste of scarce energy resources. In this paper, we propose a radio resource management framework, based on software‐defined network (SDN), to overcome the inefficient radio resource allocation of LPWA technologies. This is possible through the centralized nature of SDN, which allows collecting network monitoring information to analyze and calculate the optimal channel assignment configuration across the IoT network. We perform software‐defined radio‐based spectrum monitoring within the real IoT network platform in 868 MHz bands in which the latest IoT technologies, ie, LoRa and SigFox, operate. We demonstrate, through extensive simulations, that the proposed approach provides a better radio resource allocation for LPWA, reduces the number of packet collisions, and significantly improves the energy efficiency of the IoT communications.
Primary auditory cortex representation of fear‐conditioned musical sounds
Authors: Staib Matthias , Abivardi Aslan , Bach Dominik R. 
Published: October 2019

AbstractAuditory cortex is required for discriminative fear conditioning beyond the classical amygdala microcircuit, but its precise role is unknown. It has previously been suggested that Heschl's gyrus, which includes primary auditory cortex (A1), but also other auditory areas, encodes threat predictions during presentation of conditioned stimuli (CS) consisting of monophones, or frequency sweeps. The latter resemble natural prosody and contain discriminative spectro‐temporal information. Here, we use functional magnetic resonance imaging (fMRI) in humans to address CS encoding in A1 for stimuli that contain only spectral but no temporal discriminative information. Two musical chords (complex) or two monophone tones (simple) were presented in a signaled reinforcement context (reinforced CS+ and nonreinforced CS−), or in a different context without reinforcement (neutral sounds, NS1 and NS2), with an incidental sound detection task. CS/US association encoding was quantified by the increased discriminability of BOLD patterns evoked by CS+/CS−, compared to NS pairs with similar physical stimulus differences and task demands. A1 was defined on a single‐participant level and based on individual anatomy. We find that in A1, discriminability of CS+/CS− was higher than for NS1/NS2. This representation of unconditioned stimulus (US) prediction was of comparable magnitude for both types of sounds. We did not observe such encoding outside A1. Different from frequency sweeps investigated previously, musical chords did not share representations of US prediction with monophone sounds. To summarize, our findings suggest decodable representation of US predictions in A1, for various types of CS, including musical chords that contain no temporal discriminative information.
Pembrolizumab‐induced myasthenia gravis with myositis and presumable myocarditis in a patient with bladder cancer
Authors: Todo Maki , Kaneko Gou , Shirotake Suguru , Shimada Yuki , Nakano Shintaro , Okabe Takashi , Ishikawa Shiho , Oyama Masafumi , Nishimoto Koshiro 
Published: October 2019

IntroductionPembrolizumab cause immune‐related adverse events. We herein report a case of advanced bladder cancer, who treated with pembrolizumab and exhibited intriguing clinical course.Case presentationA 63‐year‐old man with bladder carcinoma was treated by radical cystectomy, however, the bladder carcinoma recurred and invaded to the rectum. He was treated by combination therapy using gemcitabine and cisplatin, which were not effective for the tumor. He subsequently underwent treatment with pembrolizumab. In several 30 days, he suffered from the symptoms of myasthenia gravis. Serum levels of creatine kinase, its isozyme creatine kinase‐myocardial band, and troponin I were elevated. Electrocardiography showed a right bundle branch block. These findings suggested that he was myasthenia gravis with general myositis and presumable myocarditis. Oral prednisolone administration significantly attenuated these findings. The tumor drastically shrunk only by the single injection of pembrolizumab.ConclusionEarly intervention with corticosteroid was effective for neuromuscular complications due to pembrolizumab.
The comparison of effectiveness and safety between different biosimilars of G‐CSF in the mobilization of peripheral blood stem cells (PBSCs) for autologous transplantation (autologous peripheral blood stem cell transplantation, auto‐PBSCT)
Authors: Wicherska‐Pawłowska Katarzyna , Rybka Justyna , Prajs Iwona , Szmigiel Katarzyna , Tyc Joanna , Frączak Ewa , Biedroń Monika , Kalicińska Elżbieta , Szymczak Donata , Wróbel Tomasz 
Published: October 2019

AbstractBackgroundAutologous peripheral blood marrow stem cell transplantation (auto‐PBSCT) preceded by high‐dose chemotherapy is a well‐known method of treatment for patients with hematological cancers. Performing the procedure entails obtaining from the patient their own stem cells from peripheral blood using G‐CSF. Currently, various filgrastim biosimilars are widely used.Aim of the StudyThe purpose of this study is to compare the efficacy and safety of three different biosimilars of filgrastim in PBSC mobilization in patients with hematological malignancies.Materials and MethodsThis is a retrospective analysis of 282 patients (118 women and 164 men) who underwent stem cells mobilization for auto‐PBSCT in the Department of Hematology in Wroclaw in 2012‐2014. Three filgrastim biosimilars were used: Tevagrastim (95), Nivestim (92), and Zarzio (95). Ninety patients (32%) were diagnosed with multiple myeloma, 55 (19%) with Hodgkin's lymphoma, 90 (32%) with NHLs, 20 (7%) with acute myeloid leukemia, and 27 (10%) with another hematological cancer.ResultsThe mean number of CD34+ cells collected during the first leukapheresis was 5.95 × 106/kg for Tevagrastim, 7.08 × 106/kg for Nivestim, and 6.8 × 106/kg for Zarzio (P > .05). The necessary number of leukapheresis for patients receiving Zarzio, Nivestim, and Tevagrastim was 1.32, 1.37, and 1.66, respectively (P > .05). The percentage of effective mobilizations was 88.2% for Zarzio, 86.2% for Nivestim, and 84.9% for Tevagrastim. The side effects included bone pain and headache.ConclusionAll tested biosimilars demonstrated similar effectiveness and safety profiles in patients with hematological tumors undergoing PBSC mobilization; therefore, they can be used interchangeably.
Delayed adverse events in male plateletpheresis donors: Initial insights on donor safety
Authors: Gupta Abhaykumar M. , Bajpai Meenu 
Published: October 2019

AbstractBackgroundMinimal information is available regarding delayed adverse donor events (D‐ADEs) in plateletpheresis donors. Proactive follow up of donors for D‐ADEs is not done routinely by BTS. The aim of this study was to analyze frequency and type of D‐ADEs and its correlation with contributory factors if any.MethodsIn this prospective observational study all eligible donors were contacted by telephone twice and asked about general wellbeing and questions specific to adverse donor events (ADEs). Donors were called at 24 hours and 2 weeks after donation. The ADEs were categorized in accordance with the International Society of Blood Transfusion standard guidelines.ResultsA total of 531 donors were analyzed in the study. D‐ADEs were more common as compared to immediate ADEs (I‐ADEs) (19.21% vs 5.46%, P < .0001). The most common D‐ADEs were bruises (7.34%) and sore arms (3.58%). Localized D‐ADEs in form of bruise and hematomas were more frequent as compared to systemic D‐ADEs like fatigue and vaso‐vagal reactions (16.01% vs 3.20% P < .0001). Repeat donors had a lower incidence of systemic D‐ADEs (1.61% vs 6.96%, P = .001). Donors with weight ≤75 kg and platelet count ≤230 × 103 μL were more prone to systemic D‐ADEs (P < .05). Citrate toxicity was more common in donors with weight ≤ 75 kg (P = .002).ConclusionsPlateletpheresis procedures are relatively safer without any sequelae. D‐ADEs are more common than I‐ADEs. Localized D‐ADEs are more frequent than systemic D‐ADEs. First‐time donors are more prone to D‐ADEs than repeat donors.