bluuebunny/crossref_metadata_2025_split huggingface dataset:
Erythrocytic plasma membrane calcium ATPase (PMCA4b) variations mediate redox imbalance to determine artemisinin sensitivity in malaria
Authors: Agrohi Priya , Garg Swati , Biswas Shreeja , Maurya Preeti , Kumar Vijay , Goswami Bidhan , Sil Samir Kumar , Singh Mrigendra Pal , Chand Sunil Kumar , Shalini Sneh , Singh Om P , Dhangadamajhi Gunanidhi , Ramalingam Sivaprakash , Mallick Prashant Kumar , Singh Shailja 
Published: December 2024

AbstractArtemisinin resistance inPlasmodium falciparumremains a major public health challenge for the ongoing malaria elimination programs. Till now, artemisinin resistance is linked with the variations in the genome ofP. falciparumwhich are mainly clustered in the Pfkelch13 gene. Several studies reported artemisinin resistance in the malaria endemic areas without finding any evidence of genetic variation in parasite for this occurrence. This study interrogated whether host genetic variations have any association with artemisinin sensitivity of malaria parasite. We investigated the relationship of 1) genetic variations in the human ATP2B4 gene and 2) the resultant surface expression of plasma membrane calcium ATPase (PMCA4b) with the intraerythrocytic levels of calcium, reactive oxygen species (ROS) and the resistance towards artemisinin in the intraerythrocytic parasite. This study found negative correlation of PMCA4b expression level with intraerythrocytic calcium and ROS levels. Further in-vitro growth assays revealed that artemisinin sensitivity is reduced in the parasites growing within the RBCs having low PMCA4b and high oxidative stress. Overall, this study highlights the strong association of host PMCA4b in cellular calcium mediated redox imbalance, which significantly contributes to artemisinin resistance in malaria parasite. Hence, this is the first study to document the effect of host variations on artemisinin sensitivity of the parasite. We further emphasize that many redox modulating RBC polymorphisms that are prevalent in malaria endemic areas could influence artemisinin resistance and can serve as potential biomarkers for predicting therapeutic response. Thus, detailed population specific research may provide new insights for personalized malaria treatment and may inform future drug development strategies.SignificanceThe human host and malaria parasite share a closely interconnected relationship hence host variations could influence parasite drug resistance. This study demonstrates that reduced PMCA4b expression, the primary calcium efflux pump in RBCs, significantly increases the erythrocytic calcium and reactive oxygen species (ROS) levels, thereby decreasing theP. falciparumgrowth as well as artemisinin sensitivity. Many ROS inducing RBC polymorphisms, such as sickle cell, thalassemia, G6PD deficiency, PMCA4b variations etc. have emerged as strong malaria protective traits in several studies in malaria endemic areas. But these traits could also influence artemisinin resistance and can serve as potential biomarkers for predicting therapeutic response and highlights the importance of host erythrocytes oxidative microenvironment surveillance in monitoring antimalarial drug resistance.
Ipflufenoquin, Novel Dihydroorotate Dehydrogenase Inhibitor: Efficacy Against Strawberry Diseases and Docking Simulations via AlphaFold2 and Autodock Vina
Authors: Choi Sungyu , Son Doeun , Jang Hyeong-rok , Park Soyoon , Liu Haifeng , Lee Hyeon-gyeong , Sang Hyunkyu 
Published: December 2024

AbstractIn 2023, fungal diseases, includingBotrytis cinerea(gray mold), were isolated from various strawberry fields in Korea. Ipflufenoquin, a novel class 2 dihydroorotate dehydrogenase (DHODH) inhibitor, was applied to plant pathogenic fungal species. EC50values indicated that ipflufenoquin achieved high sensitivity inB. cinereawhile maintaining broad-spectrum activity against other fungi. Fruit and greenhouse trials confirmed its robust efficacy in gray mold diseases, including those resistant to conventional fungicides like pyraclostrobin, fluxapyroxad, and benomyl. However, the ipflufenoquin application was less effective against fungal pathogens like blossom blight and Rhizopus rot, causing shifts in the fungal community and allowing less sensitive species to emerge. Structural prediction using Alphafold2 and molecular docking simulation showed ipflufenoquin was bound to the quinone binding tunnel in DHODH and highlighted the correlation between protein-ligand binding affinity and fungicide susceptibility, with species showing higher affinities being more sensitive. This study is the first to report sensitivity of strawberry fungal species to ipflufenoquin, and this fungicide is recommended in strawberry farms against gray mold disease. Additionally, we conducted in silico predictions of fungicide sensitivity across various plant pathogenic fungi using Alphafod2 and Autodock Vina. This establishes a foundation for future computational analyses to predict the effectiveness of fungicides, offering important baseline data to enhance fungicide management strategies.
Microbial alginate foraging is conserved in geographically and taxonomically distinct ruminant microbiomes
Authors: Ferrillo Alessandra , Tingley Jeffrey P. , King Marissa L. , Kidane Alemayehu , Bajwa Barinder , Xing Xiaohui , Johannessen Tina , Lysberg Alexsander , Mydland Liv Torunn , Øverland Margareth , Reintjes Greta , Shearer Anna Y. , Klassen Leeann , Low Kristin E. , Patel Trushar R. , Terry Stephanie A. , Pope Phillip B. , Abbott D. Wade , Hagen Live H. 
Published: December 2024

ABSTRACTSeaweed plays a crucial role in carbon cycling and is expected to be a valuable resource for sustainable biomass, with applications in biofuel production, human nutrition, and animal feed. Although seaweed has historically been used as a feed source for livestock grazing near coastlines, the process by which it is digested in the rumen remains unknown. Here, we show how the brown algaeSaccharina latissimais catabolized in the rumen ecosystem of two different species usingin vivoandin vitroexperimental systems. We determined that the ruminal decomposition of alginate, a prominent component of the brown algae cell wall, requires microbial catabolic pathways complete with alginate lyases and transport proteins. Evidence of digestion was obtained through a combination of animal models, bacterial imaging, multilayered meta-omics, and enzyme biochemistry. The evolution of and implications for acquisition of ‘alginate utilization loci’ within geographically and taxonomically distinct ruminants are considered.Graphical abstractSaccharina latissimais a brown alga commonly found in the North Atlantic, Arctic and Pacific oceans.S. latissimawas collected from the west coast and Canada and Norway for microbiome studies. Alginate constitutes a substantial portion of the cell wall ofS. latissima(SL), and its digestion requires a specific set of enzymes, alginate lyases. We investigated if and howS. latissimais metabolized in geographically distinct rumen ecosystems throughin vivolamb feeding experiments (2.5 and 5% inclusion, DM basis) andin vitrocattle-based rumen simulation technique, RUSITEC, experiments (up to 50% inclusion). Evidence supporting ruminal degradation of alginate was explored using a combination of multilayered meta-omics, physiology (fluorescently labelledS. latissimahot water extracts (FLA-SLAT)) and biochemical characterization of PL6 alginate lyases.
Cofilin promotes actin turnover and flexibility to drive coordinated cell movementsin vivo
Authors: do Carmo Ana Maria , Sayo Ji Hong , Fernandez-Gonzalez Rodrigo 
Published: December 2024

AbstractEmbryos display a striking ability to repair wounds rapidly, with no inflammation or scarring. Embryonic wound healing is driven by the collective movement of the cells adjacent to the wound. The cells at the wound edge polarize actin and the molecular motor non-muscle myosin II, forming a supracellular cable around the wound that generates force and coordinates cell movements to close the lesion. Actin network contraction has been associated with the disassembly of the actin filaments that form the network. We found that the actin-severing protein cofilin and its co-factor Aip1 accumulated at the edge of epidermal wounds inDrosophilaembryos. Reducing cofilin activity slowed down wound closure by 26%, indicating that cofilin is necessary for rapid wound healing. Using quantitative microscopy, we showed that cofilin controls F-actin turnover at the wound edge, but not F-actin polarity or contractile force generation. Combining genetic and pharmacological manipulations, we found that F-actin turnover at the wound edge must be tightly regulated for wounds to close rapidly. Computational modelling suggested that cofilin may contribute to rapid wound repair by maintaining a flexible actin network at the wound edge. Consistent with this model, fluorescence fluctuation analysis revealed that F-actin networks at the wound edge were significantly more rigid when we reduced cofilin activity. Together, our results indicate that cofilin promotes F-actin turnover at the wound edge to maintain a flexible actin network and facilitate rapid contraction and wound healing.
DUX4-induced HSATII RNA accumulation drives protein aggregation impacting RNA processing pathways
Authors: Arends Tessa , Bennett Sean R. , Tapscott Stephen J. 
Published: December 2024

ABSTRACTRNA-driven protein aggregation leads to cellular dysregulation, disrupting normal cellular processes, and contributing to the development of diseases and tumorigenesis. Here, we show that double homeobox 4 (DUX4), an early embryonic transcription factor and causative gene of facioscapulohumeral muscular dystrophy (FSHD), induces the accumulation of stable intranuclear RNAs, including nucleolar RNA and human satellite II (HSATII) RNA. Stable intranuclear RNAs drive protein aggregation in DUX4-expressing muscle cells. Specifically, HSATII RNA sequesters RNA methylation factors. HSATII-YBX1 ribonucleoprotein (RNP) complex formation is mediated by HSATII double-stranded RNA and NSUN2 activity. Aberrant HSATII-RNP complexes affect RNA processing pathways, including RNA splicing. Differential splicing of genes mediated by HSATII-RNP complexes are associated with pathways known to be dysregulated by DUX4 expression. These findings highlight the broader influence of DUX4 on nuclear RNA dynamics and suggest that HSATII RNA could be a critical mediator of RNA processing regulation. Understanding the impact of HSATII-RNP formation on RNA processing provides insight into the molecular mechanisms underlying FSHD.SUMMARYArends et al. show that DUX4-induction of stable intranuclear RNA, including pericentromeric human satellite II (HSATII) repeat RNA, leads to nuclear protein aggregation. HSATII ribonucleoprotein complexes impact RNA processing downstream of DUX4 expression.
Potential mechanisms and effects of melatonin-regulated Nrf2/HO-1 pathway on acute lung injury due to formaldehyde exposure
Authors: Wang Bihong , Lv Jianguo , Xu Miao , Chang Dewei , Wu Zhe , Sun Yanling 
Published: December 2024

AbstractAcute lung injury is a topic of great interest in critical care medicine due to its high mortality rates. The lungs are the immediate target organ for formaldehyde inhalation damage. Lung damage and fibrosis are the most important outcomes of severe and acute lung disease and pose a serious threat to human health. Melatonin (MT), a natural bioactive compound with anti-inflammatory and antioxidant properties, However, it is not clear whether MT can prevent FA-induced acute lung injury (ALI). Therefore, in this study, we aimed to evaluate the protective effects of MT and the potential mechanisms against FA-induced ALI. An environmental exposure bin was used to inhale 3 mg·m3 FA-induced ALI, which was given intraperitoneally with different doses of MT (5/10/20 mg/kg) after successful modeling. In addition, rats were treated with Nrf2 inhibitor (ML385) to validate the signaling pathway. Lung function was measured, histopathological/morphological changes in lung tissue were assessed, and inflammatory expression and oxidation levels in lung tissue were detected. We observed that MT greatly alleviated the lung dysfunction, pathological lung injury, pulmonary edema and inflammatory response after successful modeling of FA. In additional, MT played a role in modulating the Nrf2/HO-1 signaling pathway, which effectively inhibit oxidative stress caused by FA-induced lung tissue injure. Moreover, we found that activation of the NF-κB pathway is associated with inflammation caused by this injury. Overall, our data suggest that MT inhibits the expression of oxidative stress and inflammation in lung tissue through the institutional or Nrf2/HO-1 pathway, alleviating FA-induced ALI.
How does immediate auditory feedback coupled to locomotion influence the spontaneous actions of rats?
Authors: Yoshida Kosuke , Wada Reo , Hakataya Shiomi , Toya Genta , Okanoya Kazuo , Koda Hiroki 
Published: December 2024

AbstractSense of agency is a subjective experience that is difficult to examine in animals and preverbal infants. In animals and infants, the sensory monitoring of self-motion, which is the basis of this sense, and the contingency between self-motion and sensory feedback are investigated. Here, we performed experiments on rats using locomotor movements and auditory feedback of pulsed sounds with real-time changes in pulse interval corresponding to the velocity of locomotion, and tested whether rats change their locomotion depending on the degree of contingency between locomotion and auditory feedback. The results showed that rats increased their locomotion distance when the pulse interval of sound changed in accordance with the velocity of locomotion. However, this effect was not long-lasting. This study provides a simple and convenient method to examine the awareness of the contingency between self-motion and auditory feedback in a wide range of animal species without long-term training, and is expected to be a useful tool for comparisons with humans.
Sustainability and its influence on the complexity of the food supply chain: a survey study
Authors: Silva Bibiana Porto da , Cassel Ricardo Augusto , Wachs Priscila , Martins Vera Lúcia Milani , Saurin Tarcisio Abreu 
Published: December 2024

PurposeTo explore the relationship between sustainability and the complexity of the food supply chain (FSC) and to promote adopting a sustainable approach that balances financial growth, environmental preservation and social well-being.Design/methodology/approachAquantitative method approach was employed using a survey of 379 respondents. Principal component analysis (PCA) was employed to evaluate the factors contributing to sustainability and their impact on the complexity of the FSC.FindingsSustainability amplifies the complexity of the FSC. Several factors that influence the complexity attributes were identified, including clean technologies, stakeholder engagement and supply chain coordination.Research limitations/implicationsHighlights the importance of adopting a sustainable approach that balances financial growth, environmental preservation and social well-being in FSCs. It also suggests the need for further research to improve understanding of the factors influencing the complexity of the FSC.Practical implicationsCompanies operating in the FSC should invest in control measures, risk identification and prevention, employee training and awareness and policies and procedures for waste management and environmental sustainability.Social implicationsHighlights the importance of social responsibility in the FSC, including all stakeholders. It also emphasizes the need to minimize negative impacts on the environment and ensure the preservation of natural resources.Originality/valueProvides a comprehensive analysis of the complexity attributes of the FSC and their relationship with sustainability factors. It offers practical and theoretical insights for companies to sustainably manage the FSC.
Stator inter-turn shortcircuit fault diagnosis of induction motor based on characteristic current
Authors: Wang Xilian , Zhou Jinhan , Qin Jiayi , Geng Min , Zhao Bo 
Published: December 2024

Purpose
This paper aims to facilitate reliable online diagnosis of early faults in the stator winding inter-turn short circuits of induction motors (IMs) under various operating conditions.

Design/methodology/approach
A novel fault characteristic component, the characteristic current amplitude, is proposed for the fault. Defined as the product of short-circuit coefficient and short-circuit current, the characteristic current is derived from the positive and negative-sequence components of the stator-side current and voltage.

Findings
Simulation models of the IMs pre- and postfault, along with an experimental platform for the motor’s inter-turn short circuit, were established. The characteristic current amplitude proves more robust against voltage unbalance and load variations, which offers enhanced reliability and sensitivity for early fault diagnosis of inter-turn short circuit in IMs stator windings.

Originality/value
A novel feature is proposed. Compared with negative-sequence current, which is considered as a traditional fault feature, the characteristic current amplitude exhibits a greater robustness against the imbalanced conditions, which simultaneously possesses the attributes of both reliability and expeditiousness in fault detection.
Adoption of AI by the HR function in the civil service
Authors: Smith Mel , Prabhakar Guru , Nisar Tahir M. , Tseng Hsiao-Ting 
Published: December 2024

PurposeThis paper uses the Technology Acceptance Model (TAM) to assess the readiness of the HR function within the UK Civil Service (CSHR) to implement AI to support performance. Academic literature in relation to AI acceptance in HR functions is currently limited, so this paper aims to establish a better understanding of the current landscape and level of ambition in this area.Design/methodology/approachA quantitative research approach was adopted to determine likely behavioral intentions of workers in the human resource (HR)function if AI were implemented, by investigating key aspects of the TAM (the perceived usefulness of AI and the transparency of the CSHR in adopting AI).FindingsWhile the results suggest that the CSHR is not ready to harness AI opportunities, employees were personally ready, despite perceiving a lack of sufficient knowledge in this area. The paper identifies that more time needs to be spent on raising awareness and upskilling the HR function before the CS can be considered fully ready to harness these opportunities.Originality/valueThe penetration of artificial intelligence (AI) technologies into the global workforce brings transformative potential to the governance structures and use of digital platforms in public sector organizations. AI is likely to play a role in the operation of HR functions and influence how they might operate in the near future.
Young or old CEOs: digital transformation level influences IT investment performance feedback of manufacturing firms
Authors: Guan Feiyang , Wang Tienan 
Published: December 2024

PurposeSince IT investment constitutes a significant portion of a firm’s budget, evaluating IT investment performance is important for both research and firms’ operations. In the digital era, there are significant disparities in the effects of IT investment on firm performance.Design/methodology/approachDrawing on the resource-based view and upper echelons theory, we investigate the effect of IT investment on the business performance of manufacturing firms. We also explore the moderating role of digital transformation and dual moderating effect of digital transformation and CEO age in this relationship.FindingsAnalyzing a sample of Chinese manufacturing firms spanning 2016–2020, we find that IT investment by Chinese manufacturing firms has a negative effect on business performance. However, this negative effect is moderated by digital transformation. Furthermore, digital transformation has a more pronounced inverse moderating influence on this negative effect for firms with older CEOs.Originality/valueHence, we reveal that the key factors for reducing the negative effect of IT investment on China’s manufacturing firms are digital transformation and the appointment of older CEOs who actively lead firms’ digital transformation.
Key structure and technology of bridge cable maintenance robot – a review
Authors: Jiang Jingang , Bai Yan Shuang , Wu Dian Hao , Yu Yan Xin , Ma Xue Feng , Lin Chuan 
Published: December 2024

Purpose
The cable inspection robot is essential in maintaining bridge cables. The purpose of this paper is to summarize the maintenance methods of bridge cables. It summarizes the advantages and disadvantages of the critical structures of the external overall frame, intermediate adhesion device, attachment mechanism and driving method of the cable inspection robot. Finally, it discusses the challenges the cable inspection robot faces and the direction of future research.

Design/methodology/approach
This paper summarizes the research progress of the cable inspection robot and details the advantages and disadvantages of critical structures such as the external frame, intermediate adhesion device, attachment mechanism, driving method and safe return device of the robot. Finally, it points out the future direction of cable inspection robots, including lightweight design, hybrid design, multi-robot cooperative work, multi-technology integration and intelligent cable inspection digital twin model.

Findings
The cables are the main load-bearing components of a bridge, and their safety is crucial. However, subjected to varying loads and environmental influences over a long period, cables are prone to damage, threatening the bridge’s stability. Cable inspection robots can comprehensively detect and repair cable damage, significantly improving efficiency and safety.

Originality/value
This paper provides a comprehensive review of the current research on cable inspection robots, enabling readers to have a comprehensive and systematic understanding of the critical structures and key technologies of cable inspection robots and providing scientific references for researchers working on cable inspection robots.