In vitro ultrastructure and biodegradation of activated plasma albumin gel derived from human samples: A prospective observational study
Authors: Houshmand Behzad , Ardakani Mohammadreza Talebi , Armandei Farshad , Moscowchi Anahita , Nazari Ahmad , Ai Jafar , Kermani Mehdi Ekhlasmand , Sabri Hamoun 
Published: December 2024

AbstractBackgroundIn soft tissue regeneration, the clinical efficacy of fibrin membranes has been a pressing concern. The key to this efficacy lies in the stability of membrane and its controlled absorption. Human serum albumin, with its influence on the formation and stability of fibrin networks, could hold the key to developing a more stable alternative. This study investigates the ultrastructure and biodegradability of plasma albumin‐activated gel, a potential game‐changer in the field.MethodsBlood samples were collected from the participants and centrifuged to obtain the concentrated growth factor. The poor platelet plasma syringe was placed inside the activated plasma albumin gel device. The ultrastructure of the membrane was examined using a scanning electron microscope (SEM). The weight difference was measured over 21 days to investigate the biodegradability of the samples.ResultsTwenty‐two samples were prepared from six individuals (three males and three females). Based on SEM images, activated albumin gel after 21 days in Hank's solution exhibited a significant decrease in density and evident signs of surface degradation. The weight was significantly reduced after 21 days (p < 0.05).ConclusionIn the present study, the investigation of the ultrastructure and biodegradability of activated albumin gel showed that, based on the observed weight difference, the amount of biodegradation is high, and it may be necessary to use a thicker membrane compared to the conventional thickness of the connective tissue graft.Key points
Enhanced stability and biocompatibility: The study highlights plasma albumin‐activated gel's potential as a soft tissue scaffold, demonstrating significant biodegradation and structural changes that support cell infiltration and nutrient exchange, essential for tissue regeneration.
Controlled degradation profile: Plasma albumin gel offers a prolonged biodegradation period compared to conventional fibrin membranes, making it suitable for applications requiring stable, long‐lasting scaffolds in soft tissue regeneration.
Future clinical applications: Findings suggest that thicker plasma albumin membranes may be needed for optimal effectiveness, paving the way for further exploration in clinical trials and animal models to validate this approach in soft tissue grafting.
Plain language summaryThis study investigates plasma albumin‐activated gel as a promising material for supporting soft tissue repair, particularly in periodontal regeneration. Traditional materials, such as fibrin membranes, are often used to aid healing, but their rapid breakdown can limit effectiveness in the body. Plasma albumin, a protein naturally found in human blood, might offer a more stable alternative by forming a longer‐lasting structure. In this study, researchers processed blood samples from participants to create the gel, examining its structure under a powerful microscope and tracking changes in weight over 21 days to assess its breakdown. Results showed that the gel gradually became less dense and more porous, allowing for cell movement and nutrient flow—both critical for tissue repair. Additionally, a significant reduction in weight indicated a controlled breakdown over time. These findings suggest that plasma albumin‐activated gel may serve as a more durable scaffold for soft tissue regeneration, potentially improving healing outcomes in periodontal applications where a stable, longer‐lasting material is needed.