bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Gatekeeping Role of ESR2 to Maintain the Primordial Follicle Reserve
Authors: Chakravarthi V Praveen , Ghosh Subhra , Roby Katherine F , Wolfe Michael W , Rumi M A Karim 
Published: March 2020

AbstractOver the entire reproductive lifespan in mammals, a fixed number of primordial follicles serve as the source of mature oocytes. Uncontrolled and excessive activation of primordial follicles can lead to depletion of the ovarian reserve. We observed that disruption of estrogen receptor β (ESR2) signaling results in increased activation of primordial follicles in Esr2-null (Esr2–/–) rats. However, follicle assembly was unaffected, and the total number of follicles remained comparable between neonatal wild-type and Esr2–/– ovaries. While the activated follicle counts were increased in Esr2–/– ovary, the number of primordial follicles were markedly decreased. Excessive recruitment of primordial follicles led to premature ovarian senescence in Esr2–/– rats and was associated with reduced levels of serum AMH and estradiol. Disruption of ESR2 signaling through administration of a selective antagonist (PHTPP) increased the number of activated follicles in wildtype rats, whereas a selective agonist (DPN) decreased follicle activation. In contrast, primordial follicle activation was not increased in the absence of ESR1, indicating that the regulation of primordial follicle activation is ESR2 specific. Follicle activation was also increased in Esr2 mutants lacking the DNA binding domain, suggesting a role for the canonical transcriptional activation function. Both primordial and activated follicles express ESR2, suggesting a direct regulatory role for ESR2 within these follicles. We also detected that loss of ESR2 augmented the activation of AKT, ERK, and mTOR pathways. Our results indicate that the lack of ESR2 upregulated both granulosa and oocyte factors, which can facilitate AKT and mTOR activation in Esr2–/– ovaries leading to increased activation of primordial follicles.
Essential Role of Syntaxin-Binding Protein-1 in the Regulation of Glucagon-Like Peptide-1 Secretion
Authors: Campbell Jhenielle R , Martchenko Alexandre , Sweeney Maegan E , Maalouf Michael F , Psichas Arianna , Gribble Fiona M , Reimann Frank , Brubaker Patricia L 
Published: March 2020

Abstract
Circadian secretion of the incretin, glucagon-like peptide-1 (GLP-1), correlates with expression of the core clock gene, Bmal1, in the intestinal L-cell. Several SNARE proteins known to be circadian in pancreatic α- and β-cells are also necessary for GLP-1 secretion. However, the role of the accessory SNARE, Syntaxin binding protein-1 (Stxbp1; also known as Munc18-1) in the L-cell is unknown. The aim of this study was to determine whether Stxbp1 is under circadian regulation in the L-cell and its role in the control of GLP-1 secretion. Stxbp1 was highly-enriched in L-cells, and STXBP1 was expressed in a subpopulation of L-cells in mouse and human intestinal sections. Stxbp1 transcripts and protein displayed circadian patterns in mGLUTag L-cells line, while chromatin-immunoprecipitation revealed increased interaction between BMAL1 and Stxbp1 at the peak time-point of the circadian pattern. STXBP1 recruitment to the cytosol and plasma membrane within 30 minutes of L-cell stimulation was also observed at this time-point. Loss of Stxbp1 in vitro and in vivo led to reduced stimulated GLP-1 secretion at the peak time-point of circadian release, and impaired GLP-1 secretion ex vivo. In conclusion, Stxbp1 is a circadian regulated exocytotic protein in the intestinal L-cell that is an essential regulatory component of GLP-1 secretion.
The lymph node at a glance – how spatial organization optimizes the immune response
Authors: Grant Spencer M. , Lou Meng , Yao Li , Germain Ronald N. , Radtke Andrea J. 
Published: March 2020

ABSTRACT
A hallmark of the mammalian immune system is its ability to respond efficiently to foreign antigens without eliciting an inappropriate response to self-antigens. Furthermore, a robust immune response requires the coordination of a diverse range of cells present at low frequencies within the host. This problem is solved, in part, by concentrating antigens, antigen-presenting cells and antigen-responsive cells in lymph nodes (LNs). Beyond housing these cell types in one location, LNs are highly organized structures consisting of pre-positioned cells within well-defined microanatomical niches. In this Cell Science at a Glance article and accompanying poster, we outline the key cellular populations and components of the LN microenvironment that are present at steady state and chronicle the dynamic changes in these elements following an immune response. This review highlights the LN as a staging ground for both innate and adaptive immune responses, while providing an elegant example of how structure informs function.
Benefit of cinnamon (Cinnamomum burmannii) in lowering total cholesterol level after consumption of high-fat containing foods in white mice (Mus musculus) models
Authors: Pulungan Annisa , Pane Yunita Sari 
Published: March 2020

Background: Hypercholesterolemia is a condition where cholesterol levels in the body exceed the normal range. In Indonesia, the development of traditional medicine is carried out by examining plants known for their medicinal qualities, including cinnamon (Cinnamomum burmannii). There are many nutritional components contained in cinnamon, such as cinnamaldehyde. This has been suggested as a substance that can reduce cholesterol and triglyceride levels. This study was conducted to determine the efficacy of cinnamon in reducing total cholesterol levels of mice (Mus musculus) given high-fat feed. Methods: This is an experimental study with a pre-post control study design. The groupings were performed by a simple random sampling method. There were five groups (n=6/group): 1) Negative control (aquadest); 2) positive control of high-fat containing food (HFC; quail's yolk); 3) HFC + cinnamon extract (CE; dose 2mg/20g body weight (BW); 4) HFC + CE (dose 4mg/20gBW); 5) HFC + CE (dose 8mg/20gBW). The study was conducted for 28 days. The intervention of CE started on day 15 and ended on day 28. Measurement of total cholesterol and BW of mice was performed on days 0, 14 and 28. Results: A decrease in the total cholesterol of mice (p = 0.001) was found in the groups that consumed CE. However, there was not a significant change between groups in mice BW (p = 0.419). Conclusions: Providing cinnamon extract (Cinnamomum burmannii) for 28 days could decrease total cholesterol levels in mice compared to those not given cinnamon extract when consuming high-fat containing foods.
Dual-polarized highly sensitive surface-plasmon-resonance-based chemical and biomolecular sensor
Authors: Rakibul Islam Mohammad , Iftekher A. N. M. , Rakibul Hasan Kazi , Nayen Md. Julkar , Bin Islam Saimon 
Published: March 2020

As the research work in surface plasmon resonance (SPR)-based photonic crystal fiber (PCF) is getting tighter, a perfectly circular-shaped PCF with elliptical air holes is proposed where the performance parameters are improved significantly. The performances among our designed elliptical, circular, and rectangular air holes are compared, and the best result is achieved with the elliptical air holes. The technique used for the investigation is the finite element method, and for the simulation of data COMSOL Multiphysics 5.3a software is used. The method covers a wider range of the optical spectrum from 0.59 to 1.05 µm. The highest confinement loss achieved through our design is 340 dB/cm. The wavelength sensitivity and amplitude sensitivity are 13,000 nm/RIU and

1189.46

R
I
U

−
1

, respectively. The sensor resolution is

7.69

×

10

−
6

for our proposed design. The proposed sensor also achieved a maximum birefringence of

2.8

×

10

−
3

, which is, to our knowledge, the highest birefringence reported so far for a PCF-SPR sensor. This enables the fiber to be operated in a dual-polarized mode. The RI for the analyte ranges from 1.33 to 1.40. Based on all the characteristics, the proposed PCF structure can be used effectively for chemical and biomolecular sensing.
How Have Members of Congress Reacted to President Trump’s Trade Policy?
Authors: Clarke Andrew J. , Jenkins Jeffery A. , Micatka Nathan K. 
Published: March 2020

AbstractWhile a number of studies in recent years explore the particularistic tendencies of presidents – targeting various benefits to districts and states that will help their and their party’s electoral prospects – little work has explored how members of Congress react to such behavior. We take some initial steps in this regard by examining how members have responded to President Donald Trump’s trade initiatives. We analyze congressional newsletter mentions of tariffs or trade as a means of exploring congressional reaction. We find a positive relationship between tariff/trade mentions and partisanship, as Republican members and members from states that Trump won in 2016 are more likely to refer to tariffs/trade in their newsletters. This represents, we argue, a different kind of credit claiming. These basic empirical patterns set the stage for more systematic analysis in the future.
The Counties that Counted: Could 2020 Repeat 2016 in the US Electoral College?
Authors: Agnew John , Shin Michael 
Published: March 2020

AbstractWe briefly trace the claim that a set of counties across the three states of Michigan, Pennsylvania, and Wisconsin in large part determined the outcome of the 2016 presidential election. Rather than the demographic characteristics of the Census as such it is the meaning that these categories (young/old, Black/White, male/female, and so on) take on in particular places in which people’s lives are grounded that drives electoral outcomes. Given that the counties in question were ones in which Obama had performed well but which Trump won in 2016 and this shift was put down to his appeal to those “left behind” in the post-2008 economy, we focus on whether or not this localized appeal can be expected to continue in 2020.
Protein import by the mitochondrial disulfide relay in higher eukaryotes
Authors: Finger Yannik , Riemer Jan 
Published: March 2020

Abstract
The proteome of the mitochondrial intermembrane space (IMS) contains more than 100 proteins, all of which are synthesized on cytosolic ribosomes and consequently need to be imported by dedicated machineries. The mitochondrial disulfide relay is the major import machinery for soluble proteins in the IMS. Its major component, the oxidoreductase MIA40, interacts with incoming substrates, retains them in the IMS, and oxidatively folds them. After this reaction, MIA40 is reoxidized by the sulfhydryl oxidase augmenter of liver regeneration, which couples disulfide formation by this machinery to the activity of the respiratory chain. In this review, we will discuss the import of IMS proteins with a focus on recent findings showing the diversity of disulfide relay substrates, describing the cytosolic control of this import system and highlighting the physiological relevance of the disulfide relay machinery in higher eukaryotes.
From cytosol to mitochondria: the beginning of a protein journey
Authors: Avendaño-Monsalve Maria Clara , Ponce-Rojas José Carlos , Funes Soledad 
Published: March 2020

Abstract
Mitochondrial protein import is one of the key processes during mitochondrial biogenesis that involves a series of events necessary for recognition and delivery of nucleus-encoded/cytosol-synthesized mitochondrial proteins into the organelle. The past research efforts have mainly unraveled how membrane translocases ensure the correct protein sorting within the different mitochondrial subcompartments. However, early steps of recognition and delivery remain relatively uncharacterized. In this review, we discuss our current understanding about the signals on mitochondrial proteins, as well as in the mRNAs encoding them, which with the help of cytosolic chaperones and membrane receptors support protein targeting to the organelle in order to avoid improper localization. In addition, we discuss recent findings that illustrate how mistargeting of mitochondrial proteins triggers stress responses, aiming to restore cellular homeostasis.
Feature-aware forecasting of large-scale time series data sets
Authors: Hartmann Claudio , Kegel Lars , Lehner Wolfgang 
Published: March 2020

Abstract
The Internet of Things (IoT) sparks a revolution in time series forecasting. Traditional techniques forecast time series individually, which becomes unfeasible when the focus changes to thousands of time series exhibiting anomalies like noise and missing values. This work presents CSAR, a technique forecasting a set of time series with only one model, and a feature-aware partitioning applying CSAR on subsets of similar time series. These techniques provide accurate forecasts a hundred times faster than traditional techniques, preparing forecasting for the arising challenges of the IoT era.
Fast paraphrase extraction in Ancient Greek literature
Authors: Pöckelmann Marcus , Dähne Janis , Ritter Jörg , Molitor Paul 
Published: March 2020

AbstractIn this paper,A shorter version of the paper appeared in German in the final report of theDigital Platoproject which was funded by the Volkswagen Foundation from 2016 to 2019. [35], [28].we present a method for paraphrase extraction in Ancient Greek that can be applied to huge text corpora in interactive humanities applications. Since lexical databases and POS tagging are either unavailable or do not achieve sufficient accuracy for ancient languages, our approach is based on pure word embeddings and the word mover’s distance (WMD) [20]. We show how to adapt the WMD approach to paraphrase searching such that the expensive WMD computation has to be computed for a small fraction of the text segments contained in the corpus, only. Formally, the time complexity will be reduced fromO(N·K3·logK)\mathcal{O}(N\cdot {K^{3}}\cdot \log K)toO(N+K3·logK)\mathcal{O}(N+{K^{3}}\cdot \log K), compared to the brute-force approach which computes the WMD between each text segment of the corpus and the search query.Nis the length of the corpus andKthe size of its vocabulary. The method, which searches not only for paraphrases of the same length as the search query but also for paraphrases of varying lengths, was evaluated on the Thesaurus Linguae Graecae®(TLG®) [25]. The TLG consists of about75·10675\cdot {10^{6}}Greek words. We searched the whole TLG for paraphrases for given passages of Plato. The experimental results show that our method and the brute-force approach, with only very few exceptions, propose the same text passages in the TLG as possible paraphrases. The computation times of our method are in a range that allows its application in interactive systems and let the humanities scholars work productively and smoothly.
Risk assessment of heavy metal pollution in water, sediment and plants in the Nile River in the Cairo region, Egypt
Authors: Al-Afify Afify D.G. , Abdel-Satar Amaal M 
Published: March 2020

Abstract
Samples of water, sediment and two native plants (Eichhornia crassipes and Ceratophyllum demersum), collected seasonally from eight sites, were analyzed to investigate the level of contamination with metals (Fe, Mn, Ni, Co, Zn, Cu, Cr, Pb and Cd) in the Nile River in the Cairo region, using heavy metal pollution and contamination indices in the case of water, and the geoaccumulation index, the pollution load index, the enrichment factor and the potential ecological risk factor in the case of sediment. The results clarified that the levels of metals among three compartments were in order: sediments > plants > water. The Nile water in Cairo is not critically polluted by the studied metals and the metal pollution index for most sites does not exceed the critical limit (< 100). Sediment samples showed a clear accumulation of Mn, Ni and Cd when compared with benchmarks cited by the Environmental Protection Agency (EPA), especially during low flow seasons. The contribution of Cd to the ecological risk assessment was about 80%, while the contribution of Ni was about 10%, reflecting that these elements originated primarily from anthropogenic sources. Eichhornia crassipes and Ceratophyllum demersum have a higher accumulation capacity for Mn, Cu and Fe compared to the other studied metals.
Physiological Basis of Noise-Induced Hearing Loss in a Tympanal Ear
Authors: Warren Ben , Fenton Georgina E. , Klenschi Elizabeth , Windmill James F.C. , French Andrew S. 
Published: March 2020

Acoustic overexposure, such as listening to loud music too often, results in noise-induced hearing loss. The pathologies of this prevalent sensory disorder begin within the ear at synapses of the primary auditory receptors, their postsynaptic partners and their supporting cells. The extent of noise-induced damage, however, is determined by overstimulation of primary auditory receptors, upstream of where the pathologies manifest. A systematic characterization of the electrophysiological function of the upstream primary auditory receptors is warranted to understand how noise exposure impacts on downstream targets, where the pathologies of hearing loss begin. Here, we used the experimentally-accessible locust ear (male,Schistocerca gregaria)to characterize a decrease in the auditory receptor's ability to respond to sound after noise exposure. Surprisingly, after noise exposure, the electrophysiological properties of the auditory receptors remain unchanged, despite a decrease in the ability to transduce sound. This auditory deficit stems from changes in a specialized receptor lymph that bathes the auditory receptors, revealing striking parallels with the mammalian auditory system.SIGNIFICANCE STATEMENTNoise exposure is the largest preventable cause of hearing loss. It is the auditory receptors that bear the initial brunt of excessive acoustic stimulation, because they must convert excessive sound-induced movements into electrical signals, but remain functional afterward. Here we use the accessible ear of an invertebrate to, for the first time in any animal, characterize changes in auditory receptors after noise overexposure. We find that their decreased ability to transduce sound into electrical signals is, most probably, due to changes in supporting (scolopale) cells that maintain the ionic composition of the ear. An emerging doctrine in hearing research is that vertebrate primary auditory receptors are surprisingly robust, something that we show rings true for invertebrate ears too.
Contribution of NPY Y5Receptors to the Reversible Structural Remodeling of Basolateral Amygdala Dendrites in Male Rats Associated with NPY-Mediated Stress Resilience
Authors: Michaelson Sheldon D. , Miranda Tapia Ana Pamela , McKinty Amanda , Silveira Villarroel Heika , Mackay James P. , Urban Janice H. , Colmers William F. 
Published: March 2020

Endogenous neuropeptide Y (NPY) and corticotrophin-releasing factor (CRF) modulate the responses of the basolateral amygdala (BLA) to stress and are associated with the development of stress resilience and vulnerability, respectively. We characterized persistent effects of repeated NPY and CRF treatment on the structure and function of BLA principal neurons in a novel organotypic slice culture (OTC) model of male rat BLA, and examined the contributions of specific NPY receptor subtypes to these neural and behavioral effects. In BLA principal neurons within the OTCs, repeated NPY treatment caused persistent attenuation of excitatory input and induced dendritic hypotrophy via Y5receptor activation; conversely, CRF increased excitatory input and induced hypertrophy of BLA principal neurons. Repeated treatment of OTCs with NPY followed by an identical treatment with CRF, or vice versa, inhibited or reversed all structural changes in OTCs. These structural responses to NPY or CRF required calcineurin or CaMKII, respectively. Finally, repeated intra-BLA injections of NPY or a Y5receptor agonist increased social interaction, a validated behavior for anxiety, and recapitulated structural changes in BLA neurons seen in OTCs, while a Y5receptor antagonist prevented NPY's effects both on behavior and on structure. These results implicate the Y5receptor in the long-term, anxiolytic-like effects of NPY in the BLA, consistent with an intrinsic role in stress buffering, and highlight a remarkable mechanism by which BLA neurons may adapt to different levels of stress. Moreover, BLA OTCs offer a robust model to study mechanisms associated with resilience and vulnerability to stress in BLA.SIGNIFICANCE STATEMENTWithin the basolateral amygdala (BLA), neuropeptide Y (NPY) is associated with buffering the neural stress response induced by corticotropin releasing factor, and promoting stress resilience. We used a novel organotypic slice culture model of BLA, complemented within vivostudies, to examine the cellular mechanisms associated with the actions of NPY. In organotypic slice cultures, repeated NPY treatment reduces the complexity of the dendritic extent of anxiogenic BLA principal neurons, making them less excitable. NPY, via activation of Y5 receptors, additionally inhibits and reverses the increases in dendritic extent and excitability induced by the stress hormone, corticotropin releasing factor. This NPY-mediated neuroplasticity indicates that resilience or vulnerability to stress may thus involve neuropeptide-mediated dendritic remodeling in BLA principal neurons.
Super-hydrophobic magnesium oxychloride cement (MOC): from structural control to self-cleaning property evaluation
Authors: Qu Z. Y. , Wang Fazhou , Liu Peng , Yu Q. L. , Brouwers H. J. H. 
Published: March 2020

AbstractIn this paper, magnesium oxychloride cement (MOC), which has a needle-like structure, is upgraded with super-hydrophobic surface using a facile method involving immersion in a FAS-ethanol solution. The influence of the molar ratios of the raw materials on the super-hydrophobic property was investigated. The phase compositions, microstructure, compressive strength, water resistance and wetting behaviour are studied in detail by X-Ray diffraction, scanning electron microscopy, a water contact angle measurement instrument, and mechanical testing. The water contact angle of as-prepared MOC reaches 152 ± 1° for the optimal mix design. The variation of the water contact angle of different mixes can be explained by the Cassie–Baxter model. The experiments using rolling off dust on the super hydrophobic surface present excellent self-cleaning ability. Moreover, proposed super hydrophobic surface exhibited excellent UV-durability, indicating a promising potential for the outdoor application.