bluuebunny/crossref_metadata_2025_split huggingface dataset:
Iron imaging in myocardial infarction reperfusion injury
Authors: Moon Brianna F. , Iyer Srikant Kamesh , Hwuang Eileen , Solomon Michael P. , Hall Anya T. , Kumar Rishabh , Josselyn Nicholas J. , Higbee-Dempsey Elizabeth M. , Tsourkas Andrew , Imai Akito , Okamoto Keitaro , Saito Yoshiaki , Pilla James J. , Gorman Joseph H. , Gorman Robert C. , Tschabrunn Cory , Keeney Samuel J. , Castillero Estibaliz , Ferrari Giovanni , Jockusch Steffen , Wehrli Felix W. , Shou Haochang , Ferrari Victor A. , Han Yuchi , Gulhane Avanti , Litt Harold , Matthai William , Witschey Walter R. 
Published: June 2020

AbstractRestoration of coronary blood flow after a heart attack can cause reperfusion injury potentially leading to impaired cardiac function, adverse tissue remodeling and heart failure. Iron is an essential biometal that may have a pathologic role in this process. There is a clinical need for a precise noninvasive method to detect iron for risk stratification of patients and therapy evaluation. Here, we report that magnetic susceptibility imaging in a large animal model shows an infarct paramagnetic shift associated with duration of coronary artery occlusion and the presence of iron. Iron validation techniques used include histology, immunohistochemistry, spectrometry and spectroscopy. Further mRNA analysis shows upregulation of ferritin and heme oxygenase. While conventional imaging corroborates the findings of iron deposition, magnetic susceptibility imaging has improved sensitivity to iron and mitigates confounding factors such as edema and fibrosis. Myocardial infarction patients receiving reperfusion therapy show magnetic susceptibility changes associated with hypokinetic myocardial wall motion and microvascular obstruction, demonstrating potential for clinical translation.
Leaf Nutrient Resorption in Lucerne Decreases with Relief of Relative Soil Nutrient Limitation under Phosphorus and Potassium Fertilization with Irrigation
Authors: Yang Mei , Lu Jiaoyun , Liu Minguo , Lu Yixiao , Yang Huimin 
Published: June 2020

AbstractLeaf nutrient resorption is an important mechanism in adapting to adverse environments. However, few studies examine how nutrient resorption responds to phosphorus (P) and potassium (K) fertilization or to a shift in nutrient limitation due to water supply and fertilization. On the Loess Plateau of China, we treated lucerne (Medicago sativa L.) with P, K, or combined P and K fertilizer and three levels of water supply. The resorption efficiency of leaf P (PRE) and K (KRE) decreased with increasing water supply, whereas that of N (NRE) was unaffected. The water supply regulated the effects of P and K fertilization on resorption efficiency. With low water, P fertilization reduced NRE and significantly increased KRE. Potassium fertilization did not affect KRE and NRE, whereas PRE was significantly affected. NRE increased with increasing green leaf N:K ratio, whereas KRE and PRE decreased with increasing K:P and N:P ratios, respectively. Water supply significantly increased soil nutrient availability interactively with P or K fertilization, leading to a shift in relative nutrient limitation, which was essential in regulating nutrient resorption. Thus, lucerne growth was not limited by K but by P or by P and N, which P fertilization and water supply ameliorated.
Finding undiagnosed patients with hepatitis C infection: an application of artificial intelligence to patient claims data
Authors: Doyle Orla M. , Leavitt Nadejda , Rigg John A. 
Published: June 2020

AbstractHepatitis C virus (HCV) remains a significant public health challenge with approximately half of the infected population untreated and undiagnosed. In this retrospective study, predictive models were developed to identify undiagnosed HCV patients using longitudinal medical claims linked to prescription data from approximately ten million patients in the United States (US) between 2010 and 2016. Features capturing information on demographics, risk factors, symptoms, treatments and procedures relevant to HCV were extracted from patients’ medical history. Predictive algorithms were developed based on logistic regression, random forests, gradient boosted trees and a stacked ensemble. Descriptive analysis indicated that patients exhibited known symptoms of HCV on average 2–3 years prior to their diagnosis. The precision was at least 95% for all algorithms at low levels of recall (10%). For recall levels >50%, the stacked ensemble performed best with a precision of 97% compared with 87% for the gradient boosted trees and just 31% for the logistic regression. For context, the Center for Disease Control recommends screening in an at-risk sub-population with an estimated HCV prevalence of 2.23%. The artificial intelligence (AI) algorithm presented here has a precision which is substantially higher than the screening rates associated with recommended clinical guidelines, suggesting that AI algorithms have the potential to provide a step change in the effectiveness of HCV screening.
Declining genetic diversity of European honeybees along the twentieth century
Authors: Espregueira Themudo Gonçalo , Rey-Iglesia Alba , Robles Tascón Lucía , Bruun Jensen Annette , da Fonseca Rute R. , Campos Paula F. 
Published: June 2020

AbstractThe European honeybee (Apis mellifera) is a key pollinator and has in the last decades suffered significant population decline. A combination of factors, including decrease in genetic diversity and introduction of Varroa mites, have been suggested to be responsible for these losses, but no definitive cause has yet been appointed. In Europe not only have wild colonies been severely affected, but managed hives have had a massive decline in numbers. To test the hypothesis that honeybees’ genetic diversity has decreased in the recent past, we used reduced representation genome sequencing of 40 historical honeybee specimens collected in Natural History collections across Europe and compared them to genomic data from 40 individuals from extant populations (collected post 2006). Our results are consistent with the existence of five evolutionary lineages as previously described, and show a decrease in genetic diversity between historical and extant individuals of the same lineage, as well as high levels of admixture in historical specimens. Our data confirm that a loss of genetic diversity has occurred during the last century, potentially increasing honeybees’ vulnerability to contemporary ecological and anthropogenic stressors.
A deep learning methodology for improved breast cancer diagnosis using multiparametric MRI
Authors: Hu Qiyuan , Whitney Heather M. , Giger Maryellen L. 
Published: June 2020

AbstractMultiparametric magnetic resonance imaging (mpMRI) has been shown to improve radiologists’ performance in the clinical diagnosis of breast cancer. This machine learning study develops a deep transfer learning computer-aided diagnosis (CADx) methodology to diagnose breast cancer using mpMRI. The retrospective study included clinical MR images of 927 unique lesions from 616 women. Each MR study included a dynamic contrast-enhanced (DCE)-MRI sequence and a T2-weighted (T2w) MRI sequence. A pretrained convolutional neural network (CNN) was used to extract features from the DCE and T2w sequences, and support vector machine classifiers were trained on the CNN features to distinguish between benign and malignant lesions. Three methods that integrate the sequences at different levels (image fusion, feature fusion, and classifier fusion) were investigated. Classification performance was evaluated using the receiver operating characteristic (ROC) curve and compared using the DeLong test. The single-sequence classifiers yielded areas under the ROC curves (AUCs) [95% confidence intervals] of AUCDCE = 0.85 [0.82, 0.88] and AUCT2w = 0.78 [0.75, 0.81]. The multiparametric schemes yielded AUCImageFusion = 0.85 [0.82, 0.88], AUCFeatureFusion = 0.87 [0.84, 0.89], and AUCClassifierFusion = 0.86 [0.83, 0.88]. The feature fusion method statistically significantly outperformed using DCE alone (P < 0.001). In conclusion, the proposed deep transfer learning CADx method for mpMRI may improve diagnostic performance by reducing the false positive rate and improving the positive predictive value in breast imaging interpretation.
Sleep in honey bees is affected by the herbicide glyphosate
Authors: Vázquez Diego E. , Balbuena M. Sol , Chaves Fidel , Gora Jacob , Menzel Randolf , Farina Walter M. 
Published: June 2020

AbstractSleep plays an essential role in both neural and energetic homeostasis of animals. Honey bees (Apis mellifera) manifest the sleep state as a reduction in muscle tone and antennal movements, which is susceptible to physical or chemical disturbances. This social insect is one of the most important pollinators in agricultural ecosystems, being exposed to a great variety of agrochemicals, which might affect its sleep behaviour. The intake of glyphosate (GLY), the herbicide most widely used worldwide, impairs learning, gustatory responsiveness and navigation in honey bees. In general, these cognitive abilities are linked with the amount and quality of sleep. Furthermore, it has been reported that animals exposed to sleep disturbances show impairments in both metabolism and memory consolidation. Consequently, we assessed the sleep pattern of bees fed with a sugar solution containing GLY (0, 25, 50 and 100 ng) by quantifying their antennal activity during the scotophase. We found that the ingestion of 50 ng of GLY decreased both antennal activity and sleep bout frequency. This sleep deepening after GLY intake could be explained as a consequence of the regenerative function of sleep and the metabolic stress induced by the herbicide.
A novel protective role for microRNA-3135b in Golgi apparatus fragmentation induced by chemotherapy via GOLPH3/AKT1/mTOR axis in colorectal cancer cells
Authors: Núñez-Olvera Stephanie I. , Chávez-Munguía Bibiana , del Rocío Terrones-Gurrola María Cruz , Marchat Laurence A. , Puente-Rivera Jonathan , Ruíz-García Erika , Campos-Parra Alma D. , Vázquez-Calzada Carlos , Lizárraga-Verdugo Erik R. , Ramos-Payán Rosalío , Salinas-Vera Yarely M. , López-Camarillo César 
Published: June 2020

AbstractChemotherapy activates a novel cytoplasmic DNA damage response resulting in Golgi apparatus fragmentation and cancer cell survival. This mechanism is regulated by Golgi phosphoprotein-3 (GOLPH3)/Myo18A/F-actin axis. Analyzing the functions of miR-3135b, a small non-coding RNA with unknown functions, we found that its forced overexpression attenuates the Golgi apparatus fragmentation induced by chemotherapeutic drugs in colorectal cancer (CRC) cells. First, we found that miR-3135b is downregulated in CRC cell lines and clinical tumors. Bioinformatic predictions showed that miR-3135b could be regulating protein-encoding genes involved in cell survival, resistance to chemotherapy, and Golgi dynamics. In agreement, ectopic transfection of miR-3135b in HCT-15 cancer cells significantly inhibited cell proliferation, sensitized cells to 5-fluoruracil (5-FU), and promoted late apoptosis and necrosis. Also, miR-3135b overexpression impaired the cell cycle progression in HCT-15 and SW-480 cancer cells. Because GOLPH3, a gene involved in maintenance of Golgi structure, was predicted as a potential target of miR-3135b, we studied their functional relationships in response to DNA damage induced by chemotherapy. Immunofluorescence and cellular ultrastructure experiments using antibodies against TGN38 protein, a trans-Golgi network marker, showed that 5-FU and doxorubicin treatments result in an apoptosis-independent stacks dispersal of the Golgi ribbon structure in both HCT-15 and SW-480 cells. Remarkably, these cellular effects were dramatically hindered by transfection of miR-3135b mimics. In addition, our functional studies confirmed that miR-3135b binds to the 3′-UTR of GOLPH3 proto-oncogene, and also reduces the levels of p-AKT1 (Ser473) and p-mTOR (Ser2448) signaling transducers, which are key in cell survival and autophagy activation. Moreover, we found that after treatment with 5-FU, TGN38 factor coimmunolocalizes with beclin-1 autophagic protein in discrete structures associated with the fragmented Golgi, suggesting that the activation of pro-survival autophagy is linked to loss of Golgi integrity. These cellular effects in autophagy and Golgi dispersal were reversed by miR-3135b. In summary, we provided experimental evidence suggesting for the first time a novel role for miR-3135b in the protection of chemotherapy-induced Golgi fragmentation via GOLPH3/AKT1/mTOR axis and protective autophagy in colorectal cancer cells.
Suture type used for perineal injury repair and sexual function: a randomised controlled trial
Authors: Martínez-Galiano Juan Miguel , Arredondo-López Beatriz , Hidalgo-Ruiz Manuel , Narvaez-Traverso Alicia , Lopez-Morón Inmaculada , Delgado-Rodriguez Miguel 
Published: June 2020

AbstractThe type of suture used to repair perineal injury may be associated with this healing process and subsequent sexual function. This study aims to assess whether the suture technique used (continuous or interrupted) has an impact on a woman’s sexual function following childbirth. A single-blind randomised clinical trial was conducted with primiparous women who had experienced a perineal injury during childbirth. A computer-generated random number table was applied to allocate women to each group. Data were collected on sociodemographic variables, variables associated with childbirth, and outcomes during the 3 months after childbirth. Mean difference was used to assess the influence of the suture type on outcomes. Multivariate analyses were carried out to adjust for unbalanced variables after randomisation. Seventy women participated in the intervention group (continuous suture) and 64 women in the control group (interrupted suture). The women in the intervention group scored high for sexual desire, adjusted mean difference (aMD) = 1.8, 95% CI = 1.1–2.6 (p < 0.001); the same happened with arousal (aMD = 1.7, 95% CI = 0.8–2.5, p < 0.001). In the intervention group, orgasm was more easily reached, aMD = 0.8, 95% CI = 0.4–1.1 (p < 0.001). Women who received a continuous suture indicated they felt less discomfort (p < 0.001). Women who had a continuous suture reported better postpartum sexual function.Trial registration: ClinicalTrials.gov NCT03825211 posted 31/01/ 2019.
Updated X-ray view of the Hyades cluster
Authors: Freund S. , Robrade J. , Schneider P. C. , Schmitt J. H. M. M. 
Published: June 2020

Aims. We revisit the X-ray properties of the main sequence Hyades members and the relation between X-ray emission and stellar rotation.
Methods. As an input catalog for Hyades members, we combined three recent Hyades membership lists derived from Gaia DR2 data that include the Hyades core and its tidal tails. We searched for X-ray detections of the main sequence Hyades members in the ROSAT all-sky survey, and pointings from ROSAT, the Chandra X-Ray Observatory, and XMM-Newton. Furthermore, we adopted rotation periods derived from Kepler’s K2 mission and other resources.
Results. We find an X-ray detection for 281 of 1066 bona fide main sequence Hyades members and provide statistical upper limits for the undetected sources. The majority of the X-ray detected stars are located in the Hyades core because of its generally smaller distance to the Sun. F- and G-type stars have the highest detection fraction (72%), while K- and M-type dwarfs have lower detection rates (22%). The X-ray luminosities of the detected members range from ∼2 × 1027 erg s−1 for late M-type dwarfs to ∼2 × 1030 erg s−1 for active binaries. The X-ray luminosity distribution functions formally differ for the members in the core and tidal tails, which is likely caused by a larger fraction of field stars in our Hyades tails sample. Compared to previous studies, our sample is slightly fainter in X-rays due to differences in the Hyades membership list used; furthermore, we extend the X-ray luminosity distribution to fainter luminosities. The X-ray activity of F- and G-type stars is well defined at FX/Fbol ≈ 10−5. The fractional X-ray luminosity and its spread increases to later spectral types reaching the saturation limit (FX/Fbol ≈ 10−3) for members later than spectral type M3. Confirming previous results, the X-ray flux varies by less than a factor of three between epochs for the 104 Hyades members with multiple epoch data, significantly less than expected from solar-like activity cycles. Rotation periods are found for 204 Hyades members, with about half of them being detected in X-rays. The activity-rotation relation derived for the coeval Hyades members has properties very similar to those obtained by other authors investigating stars of different ages.
Propargylimine in the laboratory and in space: millimetre-wave spectroscopy and its first detection in the ISM
Authors: Bizzocchi L. , Prudenzano D. , Rivilla V. M. , Pietropolli-Charmet A. , Giuliano B. M. , Caselli P. , Martín-Pintado J. , Jiménez-Serra I. , Martín S. , Requena-Torres M. A. , Rico-Villas F. , Zeng S. , Guillemin J.-C. 
Published: June 2020

Context. Small imines containing up to three carbon atoms are present in the interstellar medium (ISM). As alkynyl compounds are abundant in this medium, propargylimine (2-propyn-1-imine, HC ≡C−CH =NH) thus represents a promising candidate for a new interstellar detection.
Aims. The goal of the present work is to perform a comprehensive laboratory investigation of the rotational spectrum of propargylimine in its ground vibrational state in order to obtain a highly precise set of rest frequencies and to search for it in space.
Methods. The rotational spectra of E and Z geometrical isomers of propargylimine have been recorded in the laboratory in the 83–500 GHz frequency interval. The measurements have been performed using a source-modulation millimetre-wave spectrometer equipped with a pyrolysis system for the production of unstable species. High-level ab initio calculations were performed to assist the analysis and to obtain reliable estimates for an extended set of spectroscopic quantities. We searched for propargylimine at 3 mm and 2 mm in the spectral survey of the quiescent giant molecular cloud G+0.693-0.027 located in the central molecular zone, close to the Galactic centre.
Results. About 1000 rotational transitions have been recorded for the E- and Z-propargylimine, in the laboratory. These new data have enabled the determination of a very accurate set of spectroscopic parameters including rotational, quartic, and sextic centrifugal distortion constants. The improved spectral data allowed us to perform a successful search for this new imine in the G+0.693-0.027 molecular cloud. Eighteen lines of Z-propargylimine were detected at level >2.5σ, resulting in a column-density estimate of N = (0.24 ± 0.02) × 1014 cm−2. An upper limit was retrieved for the higher energy E isomer, which was not detected in the data. The fractional abundance (with respect to H2) derived for Z-propargylimine is 1.8 × 10−10. We discuss the possible formation routes by comparing the derived abundance with those measured in the source for possible chemical precursors.
Determining the dynamics and magnetic fields in He I 10830 Å during a solar filament eruption
Authors: Kuckein C. , González Manrique S. J. , Kleint L. , Asensio Ramos A. 
Published: June 2020

Aims. We investigate the dynamics and magnetic properties of the plasma, including the line-of-sight velocity (LOS) and optical depth, as well as the vertical and horizontal magnetic fields, belonging to an erupted solar filament.
Methods. The filament eruption was observed with the GREGOR Infrared Spectrograph at the 1.5-meter GREGOR telescope on July 3, 2016. We acquired three consecutive full-Stokes slit-spectropolarimetric scans in the He I 10830 Å spectral range. The Stokes I profiles were classified using the machine learning k-means algorithm and then inverted with different initial conditions using the HAZEL code.
Results. The erupting-filament material presents the following physical conditions: (1) ubiquitous upward motions with peak LOS velocities of ∼73 km s−1; (2) predominant large horizontal components of the magnetic field, on average, in the range of 173−254 G, whereas the vertical components of the fields are much lower, on average between 39 and 58 G; (3) optical depths in the range of 0.7−1.1. The average azimuth orientation of the field lines between two consecutive raster scans (<2.5 min) remained constant.
Conclusions. The analyzed filament eruption belongs to the fast rising phase, with total velocities of about 124 km s−1. The orientation of the magnetic field lines does not change from one raster scan to the other, indicating that the untwisting phase has not yet started. The untwisting appears to start about 15 min after the beginning of the filament eruption.
Prevention of Venous Thromboembolism during Pregnancy and the Puerperium with a Special Focus on Women with Hereditary Thrombophilia or Prior VTE—Position Paper of the Working Group in Women's Health of the Society of Thrombosis and Haemostasis (GTH)
Authors: Hart Christina , Bauersachs Rupert , Scholz Ute , Zotz Rainer , Bergmann Frauke , Rott Hannelore , Linnemann Birgit 
Published: June 2020

AbstractVenous thromboembolism (VTE) is a major cause of maternal morbidity during pregnancy and the postpartum period. Because there is a lack of adequate study data, management strategies for the prevention of VTE during pregnancy have mainly been deduced from case–control and observational studies and extrapolated from recommendations for non-pregnant patients. The decision for or against pharmacologic thromboprophylaxis must be made on an individual basis weighing the risk of VTE against the risk of adverse side effects such as severe bleeding complications. A comprehensive, multidisciplinary approach is often essential as the clinical scenario is made more complex by the specific obstetric context, especially in the peripartum period. As members of the Working Group in Women's Health of the Society of Thrombosis and Haemostasis (GTH), we summarize the evidence from the available literature and aim to establish a more uniform strategy for VTE risk assessment and thromboprophylaxis in pregnancy and the puerperium. In this document, we focus on women with hereditary thrombophilia, prior VTE and the use of anticoagulants that can safely be applied during pregnancy and the lactation period.