bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening of 154 Veterinary Drug Residues in Foods of Animal Origin Using LC–MS/MS: First Action 2020.04
Authors: Desmarchelier Aurélien , Bessaire , Thomas , Savoy Marie-Claude , Tarres Adrienne , Mujahid Claudia , Beck Andrea , Mottier Pascal , Delatour Thierry 
Published: December 2020

Abstract

Background
Veterinary drug residues in food are substances (&gt;200 compounds) exhibiting potential health risks for consumers, thus being regulated in national legislations and the Codex Alimentarius. Most of the compounds are regulated based upon a maximum residue limit (MRL) while a few of them are banned in food for humans. The food sector needs a reliable and consensus analytical platform able to monitor these substances in a wide range of food commodities.

Objective
Several confirmatory methods based on liquid chromatography-mass spectrometry are available in the literature for either screening or quantification of veterinary drug residues in food, but usually applicable to limited scope of matrices. The current work describes the single-laboratory validation (SLV) of a method for screening 154 veterinary drug residues in several food categories.

Methods
This work describes a streamlined platform making use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for screening 105 antibiotics, 41 antiparasitics, 5anti-inflammatory agents, and 3 tranquilizers in foods of animal origin. For the best performance across the commodities (dairy-, meat-, fish-, and egg-based materials), four method streams were established. As a screening tool, probabilities of detection (PODs) were assessed at the screening target concentration (STC &lt; MRL) and the blank.

Results
The SLV led to PODs at the STC &gt;94% and PODs in the blank &lt; 4%.

Conclusion
Performance is in agreement with the acceptance criteria defined in SMPR 2018.010.

Highlights
The Expert Review Panel approved the present method as AOAC Official First Action 2020.04.
Identification and analysis of short open reading frames (sORFs) in the initially annotated noncoding RNA LINC00493 from human cells
Authors: Yeasmin Fouzia , Imamachi Naoto , Tanu Tanzina , Taniue Kenzui , Kawamura Takeshi , Yada Tetsushi , Akimitsu Nobuyoshi 
Published: December 2020

Abstract
Whole transcriptome analyses have revealed that mammalian genomes are massively transcribed, resulting in the production of huge numbers of transcripts with unknown functions (TUFs). Previous research has categorized most TUFs as noncoding RNAs (ncRNAs) because most previously studied TUFs do not encode open reading frames (ORFs) with biologically significant lengths [&gt;100 amino acids (AAs)]. Recent studies, however, have reported that several transcripts harbouring small ORFs that encode peptides shorter than 100 AAs are translated and play important biological functions. Here, we examined the translational capacity of transcripts annotated as ncRNAs in human cells, and identified several hundreds of ribosome-associated transcripts previously annotated as ncRNAs. Ribosome footprinting and polysome profiling analyses revealed that 61 of them are potentially translatable. Among them, 45 were nonnonsense-mediated mRNA decay targets, suggesting that they are productive mRNAs. We confirmed the translation of one ncRNA, LINC00493, by luciferase reporter assaying and western blotting of a FLAG-tagged LINC00493 peptide. While proteomic analysis revealed that the LINC00493 peptide interacts with many mitochondrial proteins, immunofluorescence assays showed that its peptide is mitochondrially localized. Our findings indicate that some transcripts annotated as ncRNAs encode peptides and that unannotated peptides may perform important roles in cells.
Population-Mediated Responses of Lasioderma serricorne (Coleoptera: Anobiidae) to Different Diagnostic Protocols for Phosphine Efficacy
Authors: Sakka Maria K , Athanassiou Christos G 
Published: December 2020

Abstract
We evaluated different monitoring protocols for phosphine resistance in adults of three populations of the cigarette beetle, Lasioderma serricorne (F.), termed LB, 2KT, and E1. The protocols evaluated were as follows: 1) the Food and Agriculture Organization (FAO) bioassay, i.e., the exposure at 30 ppm of phosphine for 20 h, 2) the dose response, based on the exposure at 50–1,000 ppm of phosphine for 3 d, 3) the Cooperation Center for Scientific Research Relative to Tobacco (CORESTA) bioassay, which is based on exposures of 4 d at 200 ppm, and, if there are surviving individuals, of 10 d at 700 ppm, and 4) Detia Degesch Phosphine Tolerance Test Kit (DDPTTK), based on the evaluation of the exposed insects for short intervals of exposure (in our case 90 min) at 3,000 ppm. From the populations tested, E1 indicated the highest survival in the FAO bioassay. Moreover, adults of this population were able to survive at 200 ppm, in the dose response, in contrast with the other populations. However, both 2KT and E1 gave similar results in DDPTTK, but only E1 had survivors after 7 d postexposure. Finally, only adults of E1 survived the CORESTA bioassay for 200 ppm and 4 d, but not for 700 ppm and 10 d. Our results show that, at least in the range of the populations tested here, there are some trends that are comparable among the different diagnostics for L. serricorne, which can be utilized further in designing a widely adopted standardized protocol.
Temporal Occurrence of Plusiinae on Soybean in the Mississippi River Delta
Authors: Allen K Clint , Little Nathan S , Perera Omaththage P 
Published: December 2020

Abstract
The subfamily Plusiinae of the moth family Noctuidae is made up 400 species worldwide. Two species of the subfamily, the soybean looper, Chrysodeixis includens (Walker), and the cabbage looper, Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae), are important defoliating insect pests of various field crops and have been the subject of previous Plusiinae surveys in the Southern U.S. Soybean fields were sampled in the Mississippi Delta from 2010 to 2012 to determine the temporal occurrence of various Plusiinae species on soybean. As in previous surveys, C. includens was the most common Plusiinae species in soybean during the 3-yr survey, especially in late season collections (July–September). Rachiplusia ou (Guenée) (Lepidoptera: Noctuidae) was the predominant species observed in early season collections (May–early July). Populations of R. ou during the first sample dates during 2010 were much higher than those observed during the other years of the survey. Only three collected larvae successfully developed into T. ni adults, one each collected during May, June, and July. Although R. ou was not commonly reported in previous studies on soybean, it occurred in low numbers during June and July during the 3-yr study. The temporal occurrence and species composition followed a predictable pattern in all 3 yr of the study.
Evaluation of Larval Therapy Compared to Antibiotic Therapy in the Treatment of Skin Wounds in Rabbits
Authors: Barros Luciano Antunes , Sant’Anna Lucas Xavier , Lessa Claudia Soares , Aguiar Coelho Valéria Magalhães , Nunes Mariana dos Passos , de Souza Cláudio Maurício Vieira , Holanda Duarte Maria Carmela Kasnowski , Fonseca Ana Beatriz Monteiro , Sakamoto Cláudio Alessandro Massamitsu , Leite Juliana da Silva 
Published: December 2020

AbstractLarval therapy (LT) is a therapeutic modality that uses larvae of necrophagous flies for the treatment of wounds. The use of this therapy presents several benefits, due to the action of the larvae that remove necrotic tissue selectively, exercising antimicrobial action, and promoting healing. There are situations in which LT proves to be the only or the best alternative for the patient, such as wounds infected by multidrug-resistant microorganisms or when treatment difficulties may lead to an indication for amputation. The purpose of this study was to compare the efficiency of LT, using larvae of Chrysomya megacephala, with that of antibiotic therapy in the treatment of cutaneous wounds infected with Pseudomonas aeruginosa. Twenty-four rabbits were used to perform the experiment, distributed in four groups: group 1, induced wound without bacterial infection; group 2, induced wound with bacterial infection; group 3, induced wound with bacterial infection and antibiotic therapy; group 4, induced wound with bacterial infection and LT. The macroscopic, microscopic, and statistical analyses indicated that LT was as effective as antibiotic therapy in wound healing.
The Nocturnal Ovipositon Behavior of Chrysomya megacephala (Diptera: Calliphoridae) in Brazil and Its Forensic Implications
Authors: Carneiro L T , Azevedo W T A , Aguiar V M , Couri M S 
Published: December 2020

Abstract
Chrysomya megacephala (Fab. 1794) (Diptera: Calliphoridae) is a very important species for forensic entomology, mainly contributing estimations of the postmortem interval (PMI) in judicial investigations. There are some doubts about the nocturnal oviposition of these flies, which could lead to errors in the PMI calculation. This study aimed to monitor the nocturnal oviposition behavior of this species through four experimental conditions carried out in laboratory. Ten cages, each containing five males and females (n = 100), were kept in a fume hood and subjected to total darkness or to artificial light for 11 consecutive hours. Two verifications were performed to determine whether the females deposited eggs on the substrate of ~20 g of chicken gizzards per cage. The first verification occurred at 9:00 pm in nocturnal experiments and at 09:00 am in diurnal experiments. The second verification occurred at 05:00 am in nocturnal experiments and at 05:00 pm in diurnal experiments. Each experiment lasted 5 d. Chrysomya megacephala deposited eggs at night under artificial light and in total darkness, but the amount of eggs was significantly lower when compared with the daytime experiments in dark conditions and under natural light. Oviposition occurred when the average temperature was around 25°C (± 2°C) and relative humidity around 73% (± 6%). Night oviposition is a possibility which should not be ruled out. Thus, future experiments are recommended.
‘Burning Violin’: The Medically Important Spider GenusLoxosceles(Araneae: Sicariidae) in Iran, Turkmenistan, and Afghanistan, With Two New Species
Authors: Zamani Alireza , Mirshamsi Omid , Marusik Yuri M 
Published: December 2020

AbstractThe taxonomic status of the medically important spider genus Loxosceles Heineken et Lowe, 1832 (Sicariidae) in Iran, Turkmenistan, and Afghanistan is revised. Two species are described as new to science: Loxosceles coheni sp. n. (♂♀, southwestern Iran) and Loxosceles turanensis sp. n. (♂♀, southern Turkmenistan and eastern Iran). Additionally, Loxosceles alicea Gertsch, 1967 syn. n. (♀, Peru) is synonymized with Loxosceles rufescens (Dufour, 1820). The local distribution of all treated species is mapped (including several new records), and reported cases of loxoscelism from this region are briefly reviewed.
Practice patterns for postoperative radiation therapy in patients with metastases to the long bones: a survey of the Japanese Radiation Oncology Study Group
Authors: Kubota Hikaru , Nakamura Naoki , Shikama Naoto , Tonari Ayako , Wada Hitoshi , Harada Hideyuki , Nagakura Hisayasu , Heianna Joichi , Ito Kei , Nozaki Miwako , Tago Masao , Fushiki Masato , Uchida Nobue , Araki Norio , Sekii Shuhei , Kosugi Takashi , Takahashi Takeo , Kawamoto Terufumi , Saito Tetsuo , Yamada Kazunari 
Published: December 2020

Abstract
Evidence regarding postoperative radiation therapy (PORT) for metastases to the long bones is lacking. Characterizing the current practice patterns and identifying factors that influence dose-fractionation schedules are essential for future clinical trials. An internet-based survey of the palliative RT subgroup of the Japanese Radiation Oncology Study Group was performed in 2017 to collect data regarding PORT prescription practices and dose-fractionation schedules. Responders were also asked to recommend dose-fractionation schedules for four hypothetical cases that involved a patient with impending pathological fractures and one of four clinical features (poor prognosis, solitary metastasis, radio-resistant primary tumor or expected long-term survival). Responders were asked to indicate their preferred irradiation fields and the reasons for the dose fractionation schedule they chose. Responses were obtained from 89 radiation oncologists (67 institutions and 151 RT plans) who used 22 dose-fractionation schedules, with the most commonly used and recommended schedule being 30 Gy in 10 fractions. Local control was the most common reason for preferring longer-course RT. High-dose fractionated schedules were preferred for oligometastasis, and low-dose regimens were preferred for patients with a poor prognosis; however, single-fraction RT was not preferred. Most respondents recommended targeting the entire orthopedic prosthesis. These results indicated that PORT using 30 Gy in 10 fractions to the entire orthopedic prosthesis is preferred in current Japanese practice and that single-fraction RT was not preferred. Oligometastasis and poor prognosis influenced the selection of high- or low-dose regimens.
Two cases of giant peritoneal inclusion cysts requiring treatment after total laparoscopic hysterectomy
Authors: Kozasa Katsumi , Takemoto Yuki , Goto Takeshi , Kobayashi Mariya , Sakaguchi Hitomi , Fujiwara Sho , Ichikawa Fuyuki , Kuroda Misako , Komura Naoko , Tanaka Asuka , Masuda Kumi , Otsuka Hirofumi , Yokoi Takeshi 
Published: December 2020

AbstractPeritoneal inclusion cysts (PICs) often develop in post-operative patients. Since the incidence of adhesions is lower with laparoscopic surgery than with open surgery, PICs are less likely to occur in the former. Although post-operative adhesions or PICs rarely develop after laparoscopic surgery (such as total laparoscopic hysterectomy: TLH), we encountered two cases of giant PICs with abdominal pain after TLH. In Case 1, strong adhesion was already present when TLH was performed. Therefore, this case may have been predisposed to the development of adhesions in the abdominal cavity. However, no adhesions were observed during TLH in case 2, and there were no risk factors, such as pre-operative adhesions and endometriosis. Therefore, adhesions and PICs may develop even after TLH, and approaches need to be considered for their prevention.
Knowledge of uncertain worlds: programming with logical constraints
Authors: Liu Yanhong A , Stoller Scott D 
Published: December 2020

Abstract
Programming with logic for sophisticated applications must deal with recursion and negation, which together have created significant challenges in logic, leading to many different, conflicting semantics of rules. This paper describes a unified language, DA logic, for design and analysis logic, based on the unifying founded semantics and constraint semantics, that supports the power and ease of programming with different intended semantics. The key idea is to provide meta-constraints, support the use of uncertain information in the form of either undefined values or possible combinations of values and promote the use of knowledge units that can be instantiated by any new predicates, including predicates with additional arguments.
About the characterization of a fine line that separates generalizations and boundary-case exceptions for the Second Incompleteness Theorem under semantic tableau deduction
Authors: Willard Dan E 
Published: December 2020

AbstractOur previous research showed that the semantic tableau deductive methodology of Fitting and Smullyan permits boundary-case exceptions to the second incompleteness theorem, if multiplication is viewed as a 3-way relation (rather than as a total function). It is known that tableau methodologies prove a schema of theorems verifying all instances of the law of the excluded middle. But if one promotes this schema of theorems into formalized logical axioms, then the meaning of the pronoun of ‘I’, used by our self-referencing engine, changes quite sharply. Our partial evasions of the second incompleteness theorem shall then come to a complete halt.
The ALMaQUEST survey – VI. The molecular gas main sequence of ‘retired’ regions in galaxies
Authors: Ellison Sara L , Lin Lihwai , Thorp Mallory D , Pan Hsi-An , Sánchez Sebastian F , Bluck Asa F L , Belfiore Francesco 
Published: December 2020

ABSTRACT
In order to investigate the role of gas in the demise of star formation on kpc-scales, we compare the resolved molecular gas main sequence (rMGMS: $\Sigma _{\rm H_2}$ versus Σ⋆) of star-forming regions to the sequence of ‘retired’ regions that have ceased to form new stars. Using data from the ALMaQUEST survey, we find that retired spaxels form a rMGMS that is distinct from that of star-forming spaxels, offset to lower $\Sigma _{\rm H_2}$ at fixed Σ⋆ by a factor of ∼5. We study the rMGMS of star-forming and retired spaxels on a galaxy-by-galaxy basis for eight individual ALMaQUEST(ALMA MaNGA QUEnching and STar formation) galaxies. Six of these galaxies have their retired spaxels concentrated within the central few kpc. Molecular gas is detected in 40–100 per cent of retired spaxels in the eight galaxies in our sample. Both the star-forming and retired rMGMS show a diversity in normalization from galaxy to galaxy. However, in any given galaxy, the rMGMS for retired regions is found to be distinct from the star-forming sequence and gas fractions of retired spaxels are up to an order of magnitude lower than the star-forming spaxels. We conclude that quenching is associated with a depletion (but not absence) of molecular gas via a mechanism that typically begins in the centre of the galaxy.
Relationship between immunoglobulin A1 lectin-binding specificities, mesangial C4d deposits and clinical phenotypes in immunoglobulin A nephropathy
Authors: Medrano Alfons Segarra , Muijsemberg Andrea , Wimbury David , Martin Marisa , Jatem Elias , González Jorge , Colás-Campás Laura , García-Carrasco Alicia , Martínez Cristina , Barratt Jonathan 
Published: December 2020

AbstractBackgroundThe reason why mesangial C4d deposits are detected in only certain biopsies of immunoglobulin A nephropathy (IGAN) remains unclear. We analyse the association between IgA glycosylation patterns, mesangial C4 deposition and clinical phenotypes in IgAN.MethodsThis cross-sectional study included 145 patients with idiopathic IgAN. We measured the serum levels of three different IgA1 lectin-binding specificities using enzyme-linked immunosorbent assays with and without treatment with neuraminidase and we analysed the relationship between these glycoforms, C4d mesangial deposits and clinical phenotypes.ResultsC4d-positive versus Cd4-negative patients had higher proteinuria [median 3.1 g/g (0.9–4.2) versus 1.8 (1–2.2); P = 0.000], haematuria [223 cells/µL (32–278) versus 99 (25–186); P = 0.000] and higher levels of IgA binding to neuraminidase untreated Helix aspersa (HA IgA1 neu−; 150.6 ± 52 U versus 96.2 ± 64.1; P = 0.000), neuraminidase untreated Helix pomatia (HPA IgA1 neu−; 0.34 ± 0.15 U versus 0.27 ± 0.13; P = 0.04), Triticum vulgaris (TV IgA1; 85.1 ± 31.7 U versus 42.2 ± 26.9; P = 0.000) and Canavalia ensiformis (ConA IgA1; 32.5 ± 18 U versus 16.7 ± 9.38; P = 0.000). The levels of HA IgA1 neu−, HPA IgA1 neu−, TV IgA1 and ConA IgA1 were all associated with the mesangial deposition of C4d, extracapillary proliferation and acute kidney injury. In receiver operating characteristics curves, HA IgA1 neu−, HPA IgA1 neu−, TV IgA1 and ConA IgA1 significantly discriminated between C4d-positive ad C4d-negative biopsies. In logistics models, TV IgA1 and ConA IgA1 were the only independent predictors of mesangial C4d deposits.ConclusionsIn IgAN, the severity of the disease is associated with the level of IgA exposing N-acetyl-d-galactosamine, N-acetyl-d-glucosamine or mannose, whereas C4d deposits are only associated with elevated levels of IgA1 glycoforms exhibiting glycan residues with specificity for mannose and N-acetyl-d-glucosamine binding lectins.
Surjective Homomorphisms from Algebras of Operators on Long Sequence Spaces are Automatically Injective
Authors: HorvÁth Bence , Kania Tomasz 
Published: December 2020

Abstract
We study automatic injectivity of surjective algebra homomorphisms from $\mathscr{B}(X)$, the algebra of (bounded, linear) operators on X, to $\mathscr{B}(Y)$, where X is one of the following long sequence spaces: c0(λ), $\ell_{\infty}^c(\lambda)$, and $\ell_p(\lambda)$ ($1 \leqslant p \lt \infty$) and Y is arbitrary. En route to the proof that these spaces do indeed enjoy such a property, we classify two-sided ideals of the algebra of operators of any of the aforementioned Banach spaces that are closed with respect to the ‘sequential strong operator topology’.
Gradient Estimates of ω-Minimizers to Double Phase Variational Problems with Variable Exponents
Authors: Byun Sun-Sig , Lee Ho-Sik 
Published: December 2020

Abstract
We are concerned with an optimal regularity for ω-minimizers to double phase variational problems with variable exponents where the associated energy density is allowed to be discontinuous. We identify basic structure assumptions on the density for the absence of Lavrentiev phenomenon and higher integrability. Moreover, we establish a local Calderón–Zygmund theory for such generalized minimizers under minimal regularity requirements regarding such double phase functionals to the frame of Lebesgue spaces with variable exponents.
Clinical Usefulness of Acute-Phase Markers in Distinguishing between PFAPA and Other Exudative Tonsillitis Causes: A Methodological Study
Authors: Kanik Ali , Eliacik Kayı , Kanik Esra Toprak , Tekindal Mustafa Agah 
Published: December 2020

AbstractBackgroundWe investigated the practical use of procalcitonin (PCT), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and complete blood count (CBC) parameters in distinguishing periodic fever, aphthous stomatitis, pharyngitis and cervical adenitis syndrome (PFAPA) attacks from exudative tonsillitis associated with group A streptococcus (GAS) and Epstein–Barre virus (EBV).MethodsThe study population consisted of cases with exudative tonsillitis who had been subsequently diagnosed as PFAPA, EBV, and GAS tonsillitis through a period of 6 years. We retrieved the CBC, ESR, CRP and PCT data from patients’ medical records.ResultsOf the patients, 47 (35.6%) had PFAPA, 36 (27.3%) had GAS and 49 (37.1%) had EBV tonsillitis. Median CRP, ESR and PCT values of patients with PFAPA were 78 (17–92) mg/dl, 44 (11–83) mm/h, 0.16 (0.01–1.45) ng/ml, respectively. The CRP and ESR levels were significantly higher in PFAPA and GAS groups compared with the EBV group (p = 0.001). There was no significant difference between the groups regarding the PCT levels.ConclusionThe study indicated no benefit of PCT in distinguishing PFAPA from the others. However, we found that CRP, ESR, and CBC parameters could be useful in identifying PFAPA and GAS than EBV tonsillitis.