bluuebunny/crossref_metadata_2025_split huggingface dataset:
Olfactory and Gustatory Dysfunctions in Patients With COVID-19 in Wuhan, China
Authors: Zou Li , Yu Ting , Zhang Yangyang , Dai Lijun , Zhang Zhaohui , Zhang Zhentao 
Published: June 2020

Abstract
Background: The coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 isspreading all over the world.The main symptoms of COVID-19 include fever, cough,fatigue, and myalgia. However, there are few reports onolfactoryand gustatory dysfunctions in patients with COVID-19.Objective: To investigate the incidence of olfactory and gustatory dysfunctions in patients with confirmed COVID-19 infection, in Wuhan, China.Methods:In this retrospective study,we collected 81 confirmed cases of COVID-19 from the Renmin Hospital of Wuhan University, from February 2020 to March 2020, and analyzed the demographic characteristics, clinical manifestations (including olfactory and gustatory dysfunctions), laboratory findings,and comorbidities.Results: A total of 81 confirmed COVID-19 patients were enrolledin this study (38 males). The most prevalent symptoms include cough, myalgia, and loss of appetite. On admission, 25 (30.9%) of all patients reported either olfactory dysfunction (OD) or gustatory dysfunction (GD), and 7 (8.6%) reported both OD and GD. 13.6% and 25.9% of allpatients reported OD and GD, respectively. OD and GD were not associated with disease severity. Pearson correlation analysisidentified some factors are positively correlated with OD and GD, including headache or dizziness (r = 0.342, P = 0.002), dark urine (r = 0.256, P = 0.021), IgM titer (r = 0.305, P = 0.01), and diabetes (r = 0.275, P = 0.013). In 81.8% of the cases with OD and 28.6% of the cases with GD, the symptomslasted for at least 1 month after discharge.3.6% of inpatients without OD developed OD after discharge.Conclusion:OD and GDare common in COVID-19.These symptoms appear early during thecourse of disease, and may last for at least 1 month.The incidence of OD and GDisrelated to neurological manifestations, diabetics, and IgM titers.
Distinct Genetic Profiles in Postpartum Depression With Different Trajectory of Illness
Authors: Takahashi Nagahide , Tainaka Hanae , Nishimura Tomoko , Harada Taeko , Okumura Akemi , Choi Damee , Iwabuchi Toshiki , Kuwabara Hitoshi , Takagai Shu , Nomura Yoko , Takei Nori , Tsuchiya Kenji. J 
Published: June 2020

Abstract
BackgroundPostpartum depression (PPD) is a common and highly heritabledisorder in the postnatal period of new mothers. The development of PPD is shown to affectneurodevelopment in children and recent evidence suggests thatthe trajectory of PPDisalso associated with children’s neurodevelopment and mental conditions. Thus, early identification and intervention for individuals at high risk of PPD are urgently needed.Additionally, it is not clear whether genetic factors affect thetrajectory of PPD. Therefore, using a polygenic risk score (PRS) approach, we investigated if PRS for depression (Depression-PRS) and bipolar disorder (Bipolar-PRS) are associated with the development and clinical course of PPD.Methods Usingrecent large genome-wide association studies(GWAS) of depression and bipolar disorder as discovery cohorts, we calculatedDepression-PRS and Bipolar-PRS in each individual. Then, we investigated the possible association between Depression-PRS and Bipolar-PRS with the development andtrajectory of PPD insubjects from the Hamamatsu Birth Cohort for mothers and children (n = 136). Depressive symptoms were assessed using the Edinburgh Postpartum Depression Scale. Gene-set enrichment analyses were used to identify pathways underlying these conditions. ResultsDepression-PRS was significantly higher in subjects with PPD than in those without PPD(t = -3.283, P = 0.002)and logistic analysis showed that Depression-PRS significantly increases therisk of developing PPD(OR [SE] = 2.274 [0.585], P = 0.002). Furthermore, Depression-PRS was positively associated with continuity of PPD (β [SE]=1.621 [0.672]; P = 0.032).Gene-set enrichment analyses revealed that pathways such as“response to hormone”(β[SE] -2.285[1.002], P < 0.001) and “epigenetic regulation”(β[SE] 2.831 [1.317], P < 0.001) were involved in the continuity of PPD. ConclusionThese preliminary findings indicate that the genetic component plays an important role not only in the development but also inthe continuity of PPD. A polygenic risk score approach could be useful to identify subjects at risk for PPD, especially for persistent PPD,who needcareful monitoring and intervention after delivery.
On the move: Exploring health service providers’ perspectives about family participation in care in Nunavik, a qualitative study.
Authors: Fraser Sarah Louise , Moulin Louise , Gaulin Dominique , Thompson Jennifer 
Published: June 2020

Abstract
BackgroundLiterature on participation in health and social services suggests that youth are difficult to engage within health and social services. Indigenous youth are less likely to access services or to actively participate in decision-making regarding their personal care. This article analyzes health and social service providers’ perspectives, experiences and expectations regarding the roles of a particular group of Indigenous youth, families and community in care settings in Nunavik, Quebec.MethodsA snowball sampling method was used to recruit participants. A total of 58 participants were interviewed including psychiatrists, general practitioners, nurses, social workers, school principals, teachers, student counsellors, representatives of local committees (education committee, health committee), and police officers. Of the 58 participants, 39 were non-Inuit and 19 were Inuit. The interviews focused on three broad themes: 1) participants’ current and past positions/roles; 2) participants’ perceptions of the clientele (youth and their families) they work with; and 3) participants’ understanding of collaborations taking place within and between services (who works with whom) and community. Applied thematic analyses were conducted. The model that we present allows us to describe health service provider expectations and experiences regarding patient and community participation. ResultsWe organize findings around three themes: I) The most commonly described interventions, II) different types of challenges to and within participation; and III) what successful participation can look like according to service providers. Participants speak of the challenges for families to go towards services as well as the challenges for services providers to go towards youth and families, including personal, organisational and historical factors. Conclusion﻿We adopt a critical lens to reflect on the key findings in order to tease out points of tension and paradoxes that might hinder the participation of youth and families. We then use a constructive lens to amalgamate and build on service providers’ descriptions of challenges and successes to identify promising approaches that seem to encourage participation of youth and families.
MicroRNA-210 Modulates Cell Migration and Invasion by Targeting Vacuole Membrane Protein 1 in U87 Glioma Cells
Authors: liu can , Liu Guangyao , Wang Chuang , Liu Guangdong , Li Jianxin , Jin Lijiao , Ma Liqiu , Ren Yanhai , Gong Haidong 
Published: June 2020

Abstract
OBJECTIVE: This study was to investigate whether the expression of vacuole membrane protein 1 (VMP1)was correlated with MicoRNA210 (miR-210) in U87 glioma cell line. MATERIALS AND METHODS: Plasmid was constructed and transfected into U87 glioma cells.The correlation between VMP1 and miR-210 was observed by the double fluorescence sumei experiment. qRT-PCR and Western blotting were used to detect the expression levels of VMP1 at mRNA and protein level, respectively.MTT assay was utilized to examine the effect of miR-210 on cell proliferation and apoptosis.The transwell chamber assay and plate cloning experiments showed the changes in the rate of cell migration and invasion after cells were transfected with miR-210. Computer SPSS 22.0 software was used to perform t-test and analysis of variance on all experimental dat and results were considered statistically significant when P < 0.05. Results: The results of the analysis of double luciferase showed that the relative fluorescence intensity of miR-210 and VMP1 in the co-staining group was significantly lower comparing to other experimental groups(P <0.001).qRT-PCR and Western blotting experiments showed that the expression level of VMP1 in miR-210 group was downregulated (P < 0.05).The MTT assay showed that the cell length curve of the miR-210 group was less comparing to the control group (P < 0.05).Transwell experiments showed that the number of cells in the miR-210 group was significantly reduced (P < 0.01).Plate cloning experiments showed no significant difference in the number of cell colonies between the experimental group and the control group (P > 0.05). Conclusion: VMP1 is a potential target gene of miR-210 in U87 glioma cell line, and provides a new option for molecular targeted therapy of gliomas in the future.
Mid-term results of total hip arthroplasty with modified trochanteric osteotomy in Crowe type IV developmental dysplasia of the hip
Authors: Jin Jing Yao , Yoon Taek Rim , Park Kyung Soon , Jin Sheng Yu , Liu Yue Ju , Li Qing Song 
Published: June 2020

Abstract
Introduction: The objective of this study was to explore mid-term clinical results of cementless total hip arthroplasty (THA) with modified trochanteric osteotomy in Crowe type IV developmental dysplasia of the hip (DDH).Patients and method: Thirteen patients (13 hips) with Crowe type IV DDH who underwent THA used modified trochanteric osteotomy between May 2013 and October 2019 were retrospectively analyzed. Mean follow-up was 5.2 ± 0.8 years (range, 4.9-6.1 years).Results: Mean Harris Hip Score (HHS) significantly (p < 0.05) improved from 30.7 ± 5.8 (range, 22-38) to 87.5 ± 3.6 (range, 83-93). The mean leg length discrepancy (LLD) was 53.4 ± 9.1 mm (range, 42.1-68.5 mm) preoperatively. The final LLD was 5.6 ± 2.4 mm (range, 2.4-9.1 mm). The mean leg length after surgery was 47.4 ± 10 mm (range, 33.6-67.2 mm). The average duration of bone union for greater trochanter (GT) was 2.5 ± 0.6 months (range, 1.5-3.6 months). There was no infection, GT non-union, or loosening (septic or aseptic) of the stem or cup in any case.Conclusions: THA with modified trochanteric osteotomy with cementless cup is an effective treatment for Crowe type IV developmental dysplasia of the hip. It can rebuild complex biomechanics and biology of hip dysplasia without increasing complications.
Does Bisphosphonate Delay Bone Healing After Proximal Femoral Nail Anti-Rotation
Authors: Phruetthiphat Ong-art , Tonvichien Sasawat , Phruetthiphat Suphot , Marsh J. Lawrence 
Published: June 2020

Abstract
BackgroundBisphosphonates are widely used for osteoporotic patients to decrease the rate of osteoporotic fractures and they have been shown to reduce the mortality rate in clinical trials. A yearly intravenous zoledronic acid in a clinical study (The HORIZON-RFT) significantly reduced any new clinical fracture and also secondary prevention of hip fracture while bisphosphonates are known to delay remodeling of bone raising the risk that they may delay fracture healing. However, current studies lack data demonstrating whether bisphosphonate (BPs) delays bone healing after hip fracture treatment in clinical practice. Purpose of study was to determine whether treating elderly patients with Bisphosphonate (BP’s) after proximal femoral nail fixation (PFNA) for intertrochanteric fractures delays fracture healing compared to similar patients not treated with BP’s. A secondary goal was to compare the functional outcome, complications and mortality between the two treatment groups.MethodsOne hundred ninety-six elderly patients underwent PFNA fixation for intertrochanteric fracture between 2015 and 2017 with age > 60 years and minimum 1-year follow-up inclusive of functional scores and complications.Intervention: Surgical treatment of intertrochanteric fracture plus calcium and vitamin D supplementation.Main Outcome Measurements: Time to clinical union and radiographic union (weeks), the functional outcome (Harris Hip Score) and complications including mechanical failure and mortality rate. Setting: Level 1 Trauma CenterResults There were comparable functional class and comorbidity between those with BP’s and not treated BP’s. In addition, there was no different in mortality rate (4.0%% vs 6.6%, p=0.537), time to clinical union (6.0 weeks vs 6.0 weeks, p=0.822), radiographic union (11.8 weeks vs 12.0 weeks, p=0.849), and functional outcome (Harris Hip Score) (74.4 vs 69.9, p=0.177) between two groups.ConclusionsBP’s can be used after surgical treatment of an osteoporotic hip fracture without concern that fracture healing will be delayed.Level of Evidence: Prognostic level III.
Strategies to Handle Increased Demand in the COVID-19 Crisis a Corona Telephone Hotline and a Web-based Self-triage System
Authors: Jensen Theo Walther , Holgersen Mathias Geldermann , Jespersen Mads Seit , Blomberg Stig Nikolaj , Folke Fredrik , Lippert Freddy Knudsen , Christensen Helle Collatz 
Published: June 2020

Abstract
BackgroundIn emergencies, such as the COVID-19 pandemic, there is an increased need for contact withemergency medical services (EMS), and call volume might surpass capacity. Thus, the Copenhagen EMS in Denmark implemented a separate coronavirus hotline followed by a web-based self-triage system to reduce nonemergency call volume. The aim of this paper is to present the two measures implemented to handle the increased call volume to the Copenhagen EMSfromthose with mild or no relevant COVID-19 symptoms.MethodsThis is a cross sectional observational study monitoring call volume in the first month of the COVID-19 pandemic in accumulated callnumbers, compared to the equivalent numbers during one month from the year before (2019). A coronavirus hotline and web-based self-triage system arepresented in absolute numbers of users.ResultsIn the first month of the COVID-19 pandemic in Copenhagen, emergency medical dispatch centers were extensively overloaded with more than 10.800 calls, resulting in significantly prolonged queue time (mean time in minutes:12:02; CI: 11:55-12:09)) compared to 2019 (mean time in minutes02:23; CI: 02:22-02:25) and thereby limiting access to emergency assistance and triage for citizens. The introduction of the coronavirus hotline showed reduced call volume and queue time to the EMS. The web-based self-triage system was used more than 107.000 times. However, no correlation between call volume and the use of a web-based self-triage systemwas observed.ConclusionsCreating a coronavirus hotlinestaffed by healthcare personnelseemed to have an impact on call volume and potentially relieved the strain in resources, while the web-based self-triage system was widely used and could be further developed to reach itsfull potential. Other EMS organizations can implement these measures to enhance capacity in a future epidemic.
A nuclear transport-related gene signature combined with IDH and 1p/19q better predicts the prognosis of glioma patients
Authors: Zhu Zheng , Lan Yang , Wang Lihong , Ge Jia , Wang Jiao , Liu Feng , He Zhicheng , Zhang Hua , Luo Min , Lin Dandan , Tan Yaoyao , Xu Yuanyuan , Luo Tao 
Published: June 2020

Abstract
Background
The nuclear transport system has been proposed to be indispensable for cell proliferation and invasion in cancers. Prognostic biomarkers and molecular targets in nuclear transport systems have been developed. However, no systematic analysis of genes related to nuclear transport in gliomas has been performed. An integrated prognostic classification involving mutation and nuclear transport gene signatures has not yet been explored.
Methods
In the present study, we analyzed gliomas from a training cohort (TCGA dataset, n = 660) and validation cohort (CGGA dataset, n = 668) to develop a prognostic nuclear transport gene signature and generate an integrated classification system. Then, we developed a nuclear transport-related risk score (NTRS) for gliomas with a training cohort.
Results
Gene set enrichment analysis (GSEA) showed that glioblastoma (GBM) was mainly enriched in nuclear transport progress compared to lower-grade glioma (LGG). NTRS was significantly correlated with clinical and genetic characteristics, including grade, age, histology, IDH status and 1p/19q codeletion, in the training and validation cohorts. Survival analysis revealed that patients with a higher NTRS exhibited shorter overall survival. NTRS showed better prognostic value compared to classical molecular markers, including IDH status and 1p/19q codeletion. Furthermore, univariate and multivariate analyses indicated that NTRS was an independent prognostic factor for gliomas. Enrichment map and Gene Ontology analysis demonstrated that signaling pathways related to the cell cycle were enriched in the NTRSHigh group. Subgroup survival analysis revealed that NTRS could differentiate the outcomes of low- and high-risk patients with wild-type IDH or mutant IDH and 1p/19q noncodeletion.
Conclusions
NTRS is associated with poor outcomes and could be an independent prognostic marker in diffuse gliomas. Prognostic classification combined with IDH mutation, 1p/19q codeletion and NTRS could better predict the survival of glioma patients.
Carbon tetrachloride (CCl4) accelerated development of non-alcoholic fatty liver disease (NAFLD)/steatohepatitis (NASH) in MS-NASH mice fed western diet supplemented with fructose (WDF)
Authors: Zhang Guodong , Wang Xiaoli , Chung Tzu-Yang , Ye Weiwei , Hodge Lauren , Zhang Likun , Chng Keefe , Xiao Yong-Fu , Wang Yixin 
Published: June 2020

Abstract
Background
Multiple NAFLD/NASH murine models have been developed by obesogenic diets and/or chemical induction. MS-NASH (formally FATZO) mouse is a spontaneously developed dysmetabolic strain that can progress from hepatosteatosis to moderate fibrosis when fed western diet supplemented with 5% fructose (WDF). This study aimed to use carbon tetrachloride (CCl4) to accelerate and aggravate progression of NAFLD/NASH in MS-NASH mouse.
Methods
Male MS-NASH mice at 8 weeks of age were fed WDF for the entire study. Starting at 16 weeks of age, CCl4 was intraperitoneally administrated twice weekly at a dose of 0.2 mL/kg for 3 weeks or 0.08 mL/kg for 8 weeks. Obeticholic acid (OCA, 30 mg/kg, QD) was administered in both MS-NASH and C57Bl/6 mice fed WDF and treated with CCl4 (0.08 mL/kg).
Results
WDF enhanced obesity and hepatosteatosis, as well as induced moderate fibrosis in MS-NASH mice similar to that reported previously. Administration of CCl4 accelerated liver fibrosis with increased bridging, but no significant impact on liver steatosis and lipid contents. Compared to the high dose CCl4 at 0.2 mL/kg, the lower dose of 0.08 mL/kg caused less death and smaller elevation of ALT and AST. Compared to MS-NASH mice, C57BI/6 mice with WDF and CCl4 (0.08 mL/kg) resulted in milder hepatosteatosis and fibrosis. OCA treatment significantly lowered liver triglycerides, steatosis and fibrosis in both MS-NASH and C57Bl/6 mice treated with WDF and CCl4.
Conclusions
CCl4 reduced induction time and exacerbated the fibrosis in MS-NASH mice on WDF, which, thus, becomes a superior NASH model with more prominent liver pathology used favorably in pharmaceutical industry for testing novel therapeutics targeting NASH.
Identification of early response to hypertonic dextrose prolotherapy markers in knee osteoarthritis patients by an inflammation-related cytokine array
Authors: Kuo Hsing-Chun , Pan Po-Jung , Wang Jia-Chi , Tsai Chih-Chun 
Published: June 2020

Abstract
BACKGROUND/AIMOsteoarthritis (OA) is one of the most common forms of arthritis, and hypertonic dextrose prolotherapy has long been used clinically to treat knee OA. The aim of this study was to investigate the inflammation-related protein-expression profile characterizing the efficacy of the hypertonic dextrose prolotherapy in knee OA as prognostic markers.METHODSOA patients over the age of 65 were recruited for Western Ontario McMaster University Osteoarthritis (WOMAC) index, knee X ray evaluation and knee joint synovial fluid analysis before and after hypertonic dextrose prolotherapy. The expressions of inflammation-related factors were measured using a novel cytokine antibody array methodology. The cytokine levels were quantified by quantitative protein expression and analyzed by ELISA using the patients’ knee-joint synovial fluid. The WOMAC Index and minimum joint space width prior to receiving the intra-articular injection and at 2-week intervals were compared.RESULTS12 patients who received OA intervention were enrolled and finally a clinical evaluation of 12 knee joints and knee synovial fluid samples were analyzed. In this study, after receiving hypertonic dextrose prolotherapy, the OA patients clearly demonstrated a significant improvement in WOMAC index and increasing tendency in the medial minimum joint space width after intervention. Meanwhile, we observed a significantly associated tendency between the high-glucose treatment of knee OA and the upregulation of MMP2, TIMP-1, EGF, CXCL9 and IL-22. These findings provide knee OA patients receiving hypertonic dextrose prolotherapy, which accompanying with the improvement of knee pain, stiffness, and function and increasing tendency in the medial minimum joint space width.
Clinical effectiveness of percutaneous endoscopic spinal surgery via transforaminal approach for single-level thoracic ossification of the posterior longitudinal ligament
Authors: Yu Qingshuai , Yang Junsong , Chu Lei , Shi Lei , Deng Zhongliang , Ke Zhenyong 
Published: June 2020

Abstract
Background

Percutaneous endoscopic spinal surgery (PESS) was performed for a series of cases with single-level thoracic ossification of the posterior longitudinal ligament (T-OPLL). This study was to evaluate the clinical feasibility and effectiveness of percutaneous endoscopic spinal surgery via a transforaminal approach for T-OPLL.
Methods

15 patients with thoracic single-level ossification of the posterior longitudinal ligament (T-OPLL) were admitted to our hospital from October 2016 to October 2019 and treated with percutaneous endoscopic spinal surgery via transforaminal approach. There were 7 males and 8 females with an average age of (56.4 ± 8.4) years (range: 41 to 71 years). Computed tomography (CT) with 3-dimensional reconstruction and magnetic resonance imaging (MRI) were used to confirm the vertebral level of T-OPLL. Levels of T-OPLL: T7/T8 in 1 case, T9/T10 in 2 cases, T10/T11 in 6 cases, T11/T12 in 4 cases, and T12/L1 in 2 cases. The surgical index, neurological function, and clinical results were recorded in this study.
Results

All surgeries were successfully completed without any conversion to open surgery, no severe intraoperative complications such as dural sac tear, major vessel or spinal cord injury were reported in our study. The average operation time was 79.1 ± 29.4 min (ranged, 43 to 132 min). The amount of intraoperative blood loss was 62.3 ± 20.8 mL. The lengths of postoperative hospital stays were 4.1 ± 1.1 days. The follow-up rate was 93.3% (14/15). The follow-up time ranged from 13 to 32 months (mean, 20.3 ± 6.3 months). the satisfactory decompression was confirmed by the CT and MRI. The Visual Analog Scale (VAS) score improved from 5.9 ± 0.7 preoperatively to 2.1 ± 0.9 at the last follow-up (P < 0.001), and Oswestry Disability Index (ODI) improved from 46.9 ± 4.5 preoperatively to 9.9 ± 8.0 at the last follow-up (P < 0.001). Neurological function was evaluated by Japanese Orthopedic Association 29 (JOA29) score, which significantly improved from 16.0 ± 1.9 to 21.2 ± 1.1 (P < 0.0001). The good and excellent rates were 71.4% according to the modified MacNab criteria. No revision surgery was performed because of the residual nucleus pulposus or recurrent disc herniation.
Conclusion

As a minimally invasive spinal technique, PESS is a clinically feasible, safe and effective alternative approach to conventional procedures in the treatment of single-level T-OPLL.