bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do Patients With Inflammatory Bowel Disease Benefit from an Enhanced Recovery Pathway?
Authors: Liska David , Bora Cengiz Turgut , Novello Matteo , Aiello Alexandra , Stocchi Luca , Hull Tracy L , Steele Scott R , Delaney Conor P , Holubar Stefan D 
Published: July 2020

Abstract

Background
Enhanced recovery pathways (ERPs) have been shown to reduce length of stay (LOS), complications, and costs after colorectal surgery; yet, little data exists regarding patients with inflammatory bowel disease (IBD). We hypothesized that implementation of ERP for IBD patients is associated with shorter LOS and improved economic outcomes.

Methods
An IRB-approved prospective clinical database was used to identify consecutive patients from 2015 to 2017. Patients were grouped as “pre-ERP” and “post-ERP” based on the date of implementation of a comprehensive ERP. Ileostomy closures, redo pouch operations, and outpatient operations were excluded. The relationship between ERP, LOS, and secondary outcomes was assessed using univariate and multivariate analysis.

Results
Overall, a total of 671 patients were included: 345 (51.4%) with Crohn’s disease (CD) and 326 (48.6%) with ulcerative colitis (UC). Of these, 425 were pre-ERP (63.4%), and 246 were post-ERP (36.6%). The groups did not differ in terms of age, gender, American Society of Anesthesiologist (ASA) scores, comorbidities, estimated blood loss, or ostomy construction. The post-ERP group had a significantly higher mean body mass index (BMI), more patients with CD, longer operative time, and more minimally invasive surgery (MIS; all P &lt; 0.05). The post-ERP group had a significantly shorter LOS (6 vs 4.5 days, median), whereas mean hospital costs decreased by 15.7%. There was no difference in readmissions or complications. On multivariate analysis, MIS and ERP use were both associated with a shorter LOS.

Conclusion
Inflammatory bowel disease patients benefit from the use of ERP, demonstrating decreased LOS and costs without an increase in complications and readmissions. Enhanced recovery pathways should be routinely implemented in this often challenging patient population.
Detex for Detection of Escherichia coli O157 in Raw Ground Beef and Raw Ground Poultry
Authors: Henry Yvette M , Natrajan Nandini , Lauer Wendy F 
Published: July 2020

Abstract
A method for detection of Escherichia coli O157 in beef and poultry is presented. The method is antibody-based and uses a patented antibody-specific metal-plating procedure for the detection of E. coli O157 in enriched meat samples. Both raw ground beef and raw ground poultry were tested as matrixes for the organism. The sensitivity and specificity of the assay were 98 and 90%, respectively. The accuracy of the assay was 96%. Overall, the method agreement between the E. coli O157 Detex assay and the U.S. Department of Agriculture/Food Safety Inspection Service method was 96%. Sample temperature upon loading of the apparatus was critical to the observed false-positive rate of the system.
A New, Sensitive, and Rapid Spectrophotometric Method for the Determination of Sulfa Drugs
Authors: Nagaraja Padmarajaiah , Yathirajan Hemmige S , Sunitha Kallanchira R , Vasantha Ramanathapura A 
Published: July 2020

Abstract
A sensitive, rapid, and simple spectrophotometric method is described for the determination of sulfa drugs. The method is based on the formation of a red-colored product by the diazotization of sulfonamides such as sulfathiazole (SFT), sulfadiazine (SFD), sulfacetamide (SFA), sulfamethoxazole (SFMx), sulfamerazine (SFMr), sulfaguanidine (SFG), and sulfamethazine (SFMt), followed by complexation with dopamine in the presence of molybdate ions in (1 + 1) H2SO4 medium. Absorbance of the resulting red product is measured at 490–510 nm, and the product is stable for 2 days at 27°C. Beer's law is obeyed in the concentration range of 0.04–8.0 μg/mL at the wavelength of maximum absorption. The method was used successfully for the determination of some sulfonamides in tablets and eye drops. Common excipients used as additives in pharmaceuticals do not interfere in the proposed method. The method offers the advantages of simplicity, rapidity, and sensitivity without the need for extraction or heating. The limits of detection and quantitation were calculated for SFT, SFD, SFA, SFMx, SFMr, SFG, and SFMt.
Reversed-Phase Liquid Chromatographic Determination of Riboflavin in Feeds
Authors: Britton Nancy L , Riter Ken L , Smallidge Robert L , Hillebrandt Joseph 
Published: July 2020

Abstract
A method for determination of riboflavin in animal feeds using liquid chromatography (LC) was developed for feed samples fortified with riboflavin at 1 mg/lb or greater (up to 10 000 mg/lb). Feed samples were extracted in 0.1N HCl with heating on a steam bath for 30 min, followed immediately by mechanical shaking for 30 min. Sample extracts were diluted to target volume with 2%acetic acid and filtered; riboflavin was determined by LC on a reversed-phase C18 column with 2% acetic acid–acetonitrile (85 + 15) mobile phase for separation and fluorescence detection with excitation at 460 nm and emission at 530 nm. The extraction was compared with that of the AOAC Official Method for riboflavin in food and feed premixes. The 2 method extractions were not significantly different from each other at the 95% confidence level. The developed method also had good linearity over 4 orders of magnitude, recovery of 95–99% from spiked feed samples, a limit of detection of riboflavin at 0.00034 μg/mL in solution, a limit of quantitation of 0.023 mg/lb in feed, and good ruggedness.
Determination of Sulfite in Dried Garlic by Reversed-Phase Ion-Pairing Liquid Chromatography with Post-Column Detection
Authors: Perfetti Gracia A , Diachenko Gregory W 
Published: July 2020

Abstract
A method is described for determining sulfite in dried garlic. Garlic is extracted with an HCl solution to inhibit the formation of allicin, which interferes with the determination of sulfite. After cleanup of the extract on a C18 solid-phase extraction column, sulfite is converted to hydroxymethylsulfonate (HMS) by adding formal-dehyde and heating to 50°C. HMS is determined by reversed-phase ion-pairing liquid chromatography with post-column detection. The post-column reaction system consists of the addition of KOH to convert HMS to sulfite ion, followed by the addition of 5,5'-dithiobis(2-nitrobenzoic acid) to produce 5-mercapto-2-nitrobenzoic acid which is detected spectrophotometrically at 450 nm. Background levels in unsulfited dried garlic equivalent to &lt;20 ppm SO2 were found. Recoveries of HMS from spiked garlic averaged 94.8% with a coefficient of variation of 3.8%. Sulfite was found in 13 of 21 samples of dried garlic produced in China, with sulfite ranging from 114 to 445 ppm. Sulfite was found in 60% of commercial dried garlic products purchased locally. The suitability of the Monier-Williams method for determining sulfite in garlic is discussed.
Impedance Method for Rapid Detection of Total Counts of Bacteria and Fungi in Bottled Purified Water
Authors: Ji-Cheng Huang , Ji-Wen Yang , Wei-Dong Lai , Man-Dang Song , Feng Deng , Jin-Yan Lin 
Published: July 2020

Abstract
A study was conducted to compare the current conventional method and the impedance method for rapid detection of total counts of bacteria and fungi in bottled purified water. The results indicated that the corresponding detection rate for the 2 methods was 94.5 and 90.4%, respectively. The detection time was shortened from 48 h (bacteria) and 5 days (fungi) to 14.5 and 44 h, respectively. Moreover, for more serious contamination, less time was needed. The impedance method predicted a positive sample within 11.3 h (bacteria) and 27.1 h (fungi). Therefore, the impedance method was found to be a rapid, accurate method.
Determination of the Presence of Bovine Immunoglobulin G in Liquid or Spray-Dried Porcine Plasma and Whole Blood by Agar Gel Immunodiffusion
Authors: Polo Javier , Saborido Neus , Ródenas Jesús , Rodríguez Carmen 
Published: July 2020

Abstract
There is currently urgent interest in identifying the species of origin of the components of different animal by-products. In Europe, this interest is expected to increase with authorization of the re-introduction of these proteins into animal feed formulations. The number of validated methods to differentiate the species of origin for most of these products is limited. An easy, inexpensive, and accurate test was developed to determine the cross-contamination of bovine blood or plasma in porcine whole blood and plasma, both before and after spray drying. Agar gel immunodiffusion (AGID), the studied technique, detected the presence of bovine immunoglobulin G (IgG) in porcine plasma and in whole blood at inclusion levels &gt;0.5% (v/v) in all cases. However, detectability was lower in liquid plasma (0.3%, v/v) and in liquid whole blood (0.5%, v/v). No differences were found when cross-contamination was simulated before or after whole blood centrifugation. The method described is reliable and inexpensive, and the samples are easy to prepare. Both minimal laboratory equipment and expertise are required for detection of bovine IgG in porcine blood products at inclusion levels of &gt;0.5% (v/v).
Analysis of Sterol Oxidation Products in Foods
Authors: Guardiola Francesc , Bou Ricard , Boatella Josep , Codony Rafael 
Published: July 2020

Abstract
The main aspects related to the analysis of sterol oxidation products (SOP) in foods are comprehensively reviewed. Special emphasis is placed on the critical and controversial points of this analysis because these points affect crucial analytical parameters such as precision, accuracy, selectivity, and sensitivity. The effect of sample preparation and the conditions of quantification by gas chromatography and liquid chromatography on these parameters are also reviewed. The results show that, in order to choose an adequate method to analyze SOP in a certain food, the analyst must consider its SOP concentration and matrix complexity. The term SOP includes both cholesterol oxidation products (COP) and phytosterol oxidation products (POP). The state of the art of COP and POP analysis is quite different; many more studies have dealt with the analysis of COP than of POP. However, most of the results presented here about COP analysis may be extrapolated to POP analysis because both groups of compounds show similar structures and characteristics.
Resistant Starch as Related to Companion Animal Nutrition
Authors: Spears Julie K , Fahey George C 
Published: July 2020

Abstract
Companion animal dietsmay contain up to 50% starch, derived from cereal grains. The amount of resistant starch (RS) in an ingredient depends on the origin and form of the ingredient and on the processing conditions to which the ingredient has been exposed. Extrusion has proven to be a means of optimizing utilization of starch by companion animals. Although the RS fraction of starch typically decreases by extrusion, retrogradation can result in increased concentrations of this fraction. Limited research exists regarding the effects of RS in companion animal nutrition and gastrointestinal health. Existing in vitro and in vivo research indicates that certain RS sources are readily fermented in the large bowel, producing short-chain fatty acids, whereas others are less fermentable, resulting in excellent laxation properties. Feeding dogs a diet high in RS may result in an increase in fecal bulk due to an increased excretion of microbial matter in those cases where RS is highly fermentable, or to indigestibility of the RS source in other cases. RS has a role to play as a potential proxy for dietary fiber, especially for those companion animals fed diets high in protein and fat and devoid of traditional dietary fiber.
Collaborative Trial for Validation of a Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay for Detection of Central Nervous System Tissues as Bovine Spongiform Encephalopathy RiskMaterial: Part 1
Authors: Abdulmawjood Amir , Schnenbrcher Holger , BÜlte Michael 
Published: July 2020

Abstract
A collaborative trial was conducted to evaluate a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for detection of central nervous system (CNS) tissues in meat products (e.g., sausages). The method is based on the detection of ruminant glial fibrillary acidic protein (GFAP) mRNA by applying real-time RT-PCR. The assay was evaluated through a multicenter trial involving 12 participating laboratories that received coded cDNA obtained from 3 different types of sausages. The participants used 5 different real-time detection systems. The results obtained in this validation revealed that this real-time RT-PCR assay performed well in the different laboratories with a detection limit of at least 0.1% CNS in those test materials that contained strongly heat-treated samples (sausages cooked at 120C) and the medium heat-treated samples (sausages cooked at 80C). The detection limit of liver sausages was determined to be 0.2% of CNS. Neither the samples with no CNS additive nor the bovine DNA and the negative control containing 100% swine brain gave any positive signals. The presented results indicate that the real-time RT-PCR assay was just as reproducible between laboratories, as repeatable within a laboratory, could reliably be used for detection of bovine spongiform encephalopathy risk material in meat and meat products, and signify that it may be used with confidence in any laboratory.
Interlaboratory Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for the Detection of Egg, Milk, Wheat, Buckwheat, and Peanut in Foods
Authors: Matsuda Rieko , Yoshioka Yasuo , Akiyama Hiroshi , Aburatani Kenichi , Watanabe Yumiko , Matsumoto Takashi , Morishita Naoki , Sato Hidetaka , Mishima Takashi , Gamo Reiko , Kihira Yasunori , Maitani Tamio 
Published: July 2020

Abstract
The labeling of 5 major allergenic ingredients (egg, milk, wheat, buckwheat, and peanut) is mandatory in Japan, and 2 series of enzyme-linked immunosorbent assay (ELISA) kits have been established as official screening methods. However, these official methods have not provided the necessary sensitivity, due in part to poor extraction efficiency. To address this need, 2 novel ELISA kits have been developed: the FASTKIT ELISA Ver. II Series and the FASPEK Allergenic Substances Detection Kit. The new kit systems use an improved extraction buffer that can extract insoluble proteins produced by processing and feature new antibodies that bind to the denatured proteins extracted with the new extraction buffer. The analytical performances of the 2 new ELISA kit series were evaluated in an interlaboratory study. Ten laboratories participated in the study and determined the major allergenic ingredients contained in 5 types of model processed food. The 2 ELISAs displayed fairly good reproducibility and sufficient recovery.
Simultaneous Determination of 17 Sulfonamides and the Potentiators Ormetoprim and Trimethoprim in Salmon Muscle by Liquid Chromatography with Tandem Mass Spectrometry Detection
Authors: Potter Ross A , Burns B Garth , Riet Jeffrey M van de , North David H , Darvesh Rozina 
Published: July 2020

Abstract
A simple, robust method using liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the simultaneous determination of 17 sulfonamides sulfanilamide (SNL), sulfacetamide (SAA), sulfaguanidine (SGD), sulfapyridine (SPY), sulfadiazine (SDZ), sulfathiazole (STZ), sulfamerazine (SMR), sulfamethoxazole (SOZ), sulfamoxole (SXL), sulfisoxazole (SXZ), sulfamethizole (SML), sulfamethazine (SMZ), sulfamethoxypyridazine (SMP), sulfamonomethoxine (SMM), sulfachloropyridazine (SCP), sulfaquinoxaline (SQX), and sulfadimethoxine (SDM) and 2 potentiators ormetoprim (OMP) and trimethoprim (TMP) in fish tissue has been developed. The analytes were extracted from homogenized fish tissue with wateracetonitrile (50 + 50). The extract was clarified by centrifugation and a portion defatted with hexane. The analytes were partitioned into chloroform and evaporated to dryness. The redissolved residue was applied to a C18 reversed-phase column with a wateracetonitrile (0.1% acetic acid) gradient. All of the compounds were completely separated and detected in &lt;10 min at 30°C using LC/MS/MS. Standard curves were linear over the range of 0.02 to 5 ng injected. The limit of detection varied from 0.1 ng/g for SMZ and OMP to 0.9 ng/g for SXL and SOZ. Recoveries varied from 100% for SDM, SOZ, and SQX and 85% for SMR, OMP, and TMP to approximately 30% for SAA. Relative standard deviations for repeat analysis varied from 4% for SMZ and SCP to 23% for SAA.
Stability-Indicating Reversed-Phase Liquid Chromatographic Method for Simultaneous Determination of Simvastatin and Ezetimibe from Their Combination Drug Products
Authors: Chaudhari Bharat G , Patel Natvarlal M , Shah Paresh B 
Published: July 2020

Abstract
A simple, precise, and rapid stability-indicating reversed-phase column liquid chromatographic (RP-LC) method has been developed and subsequently validated for simultaneous estimation of simvastatin (SIM) and ezetimibe (EZE) from their combination drug product. The proposed RP-LC method utilizes a LiChrospher 100 C18, 5 m, 250 4.0 mm id column at ambient temperature; optimum mobile phase consisting of acetonitrilewatermethanol (60 + 25 + 15, v/v/v) with apparent pH adjusted to 4.0 0.1; mobile phase flow rate of 1.5 mL/min; and ultraviolet detection at 238 nm. SIM, EZE, and their combination drug product were exposed to thermal, photolytic, hydrolytic, and oxidative stress conditions, and the stressed samples were analyzed by the proposed method. There were no other coeluting, interfering peaks from excipients, impurities, or degradation products due to variable stress conditions, and the method is specific for the estimation of SIM and EZE in the presence of degradation products. The described method was linear over the range of 180 and 380 g/mL for SIM and EZE, respectively. The mean recoveries were 99.17 and 100.43 for SIM and EZE, respectively. The intermediate precision data were obtained under different experimental conditions, and the calculated value of the coefficient of variation was found to be less than the critical value. The proposed method can be useful in the quality control of bulk manufacturing and pharmaceutical dosage forms.
Evaluation of Sample Preparation Methods for the Isolation of Salmonella from Alfalfa and Mung Bean Seeds with the Bacteriological Analytical Manual's Salmonella Culture Method
Authors: Jacobson Andrew P , Hammack Thomas S , Andrews Wallace H 
Published: July 2020

Abstract
Five pre-enrichment methods were evaluated for effectiveness with the U.S. Food and Drug Administration's Bacteriological Analytical Manual Salmonella culture method in recovering S. Stanley, S. Poona, and S. Muenchen from artificially contaminated alfalfa seeds, and S. Saintpaul, S. Anatum, and S. Infantis from artificially contaminated mung bean seeds. The methods included: (1) Soak.Test portions were inoculated into pre-enrichment media; (2) Rinse.Test portions were rinsed with pre-enrichment media, and the media was decanted from the test portions; (3) Rinsed seed.Pre-enrichment media was added to the test portions that were rinsed in the rinse method; (4) Wet blend.Test portions were blended with the pre-enrichment media; and (5) Dry blend.Test portions were blended prior to pre-enrichment. The methods of pre-enrichment were also evaluated for effectiveness in recovering Pantoea agglomerans from alfalfa and mung bean seeds with a modified culture method for the recovery of Enterobacteriaceae from foods. The purpose of these studies was to provide a model for the recovery of Salmonella that may occur in seeds as a natural contaminant. The relative effectiveness of the soak method was consistently superior to the rinse method in isolating the selected Salmonella serovars from both seed types. Statistically, the rinsed seed method was as effective as the soak method in all trials, except 1 of 3, with S. Muenchen and alfalfa seeds (P &gt; 0.05). The relative effectiveness of the methods in isolating P. agglomerans from alfalfa and mung bean seeds was similar to that observed with the artificially contaminated test portions. The soak method was consistently the most effective method and the rinse method was consistently the most ineffective method. The rinsed seed, wet blend, and dry blend methods were also as effective as the soak method in all 3 trials with each seed type (P &gt; 0.05).
Incomplete Recoveries of Fumonisins Present in Naturally Contaminated Corn Foods from an Immunoaffinity Column
Authors: Oh Keum Soon , Scott Peter M , Chung Soo-Hyun 
Published: July 2020

Abstract
Following previous observations of apparent instability of fumonisin B1 in corn starch and corn meal, immunoaffinity column (IAC) cleanup, of the type used in the analysis of commercial starch-containing corn foods for fumonisins, was investigated. Foods analyzed for naturally occurring fumonisins B1, B2, and B3 included corn flour (3 different products), corn meal, and corn flakes. In 2 series of experiments, fractions were eluted by gravity or vacuum from narrow- or wide-bore Fumonitest IACs either with 2 2 mL methanol, followed by 2 mL methanolwater (8 2, v/v), or with 2 mL methanol, then 2 mL methanolwater (8 2, v/v). The ratio () of fumonisin B1 concentration in the first methanol eluate to the total concentration measured from all eluates in most cases varied from 2570. Incomplete recoveries were also observed for fumonisins B2 and B3. It is concluded that there can be a major underestimation of naturally occurring fumonisins in methods using only methanol elution for IAC cleanup, as in AOAC INTERNATIONAL Official Method 2001.04 (accuracy of these methods had been determined only by spiking the food with fumonisins). Elution with 2 2 mL methanolwater (8 2, v/v) was chosen as a practical procedure; means of 8198 of the total fumonisin B1 concentration were found in the first eluate, except for corn flakes and 2 experiments with corn meal.
Liquid ChromatographicDiode-Array Detection Multiresidue Determination of Rice Herbicides in Drinking and Paddy-Field Water
Authors: Roehrs Rafael , Zanella Renato , Pizzuti Ionara , Adaime Martha B , Pareja Luca , Niell Silvina , Cesio Mara V , Heinzen Horacio 
Published: July 2020

Abstract
A sensitive, rapid, and simple multiresidue method for the simultaneous determination of six postemergence herbicides currently used in rice cultivationmetsulfuron methyl, bensulfuron methyl, pyrazosulfuron ethyl, bentazone, bispyribac sodium, and cyhalofop butylin drinking and paddy-field water is presented. Water samples were extracted with solid-phase extraction cartridges. Final determination was made by LC with diode-array detection. The extraction efficiencies of C18 and HLB cartridges were compared. The average recovery obtained for these compounds for the lowest spiked level (0.1 g/L) varied from 70 to 122 for C18 and 75119 for HLB, with RSDs of 11 and 8.3, respectively. The method had good linearity, and the lower detection limit for the pesticides studied varied from 0.03 to 0.04 g/L. The proposed method was also tested in paddy-field water, with recovery studies giving good results with low RSDs at 1.0 g/L.
Modern High Performance Liquid Chromatography in the Analysis of Phytochemicals
Authors: Waksmundzka-Hajnos Monika 
Published: July 2020

Abstract
Phytochemical analysis is a very important field of phytochemistry that determines the quantitative and qualitative composition of plant material. The main significance of the analysis is to show the presence of plant metabolites in herbs that are responsible for their pharmacological and/or nutritive values.