bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of an intensified fed-batch production platform with doubled titers using N-1 perfusion seed for cell culture manufacturing
Authors: Xu Jianlin , Rehmann Matthew S. , Xu Mengmeng , Zheng Shun , Hill Charles , He Qin , Borys Michael C. , Li Zheng Jian 
Published: March 2020

AbstractThe goal of cell culture process intensification is to increase volumetric productivity, generally by increasing viable cell density (VCD), cell specific productivity or production bioreactor utilization in manufacturing. In our previous study, process intensification in fed-batch production with higher titer or shorter duration was demonstrated by increasing the inoculation seeding density (SD) from ~ 0.6 (Process A) to 3–6 × 106 cells/mL (Process B) in combination with media enrichment. In this study, we further increased SD to 10–20 × 106 cells/mL (Process C) using perfusion N-1 seed cultures, which increased titers already at industrially relevant levels by 100% in 10–14 day bioreactor durations for four different mAb-expressing CHO cell lines. Redesigned basal and feed media were critical for maintaining higher VCD and cell specific productivity during the entire production duration, while medium enrichment, feeding strategies and temperature shift optimization to accommodate high VCDs were also important. The intensified Process C was successfully scaled up in 500-L bioreactors for 3 of the 4 mAbs, and quality attributes were similar to the corresponding Process A or Process B at 1000-L scale. The fed-batch process intensification strategies developed in this study could be applied for manufacturing of other mAbs using CHO and other host cells.
Newly designed multi-stacked circular tray solid-state bioreactor: analysis of a distributed parameter gas balance during solid-state fermentation with influence of variable initial moisture content arrangements
Authors: Manan Musaalbakri Abdul , Webb Colin 
Published: March 2020

AbstractBackgroundThe growth ofAspergillus awamoriandAspergillus oryzaein a self-designed, multi-stacked circular tray solid-state bioreactor (SSB), operating in solid-state fermentation (SSF) conditions at a laboratory scale, was studied. The bioreactor was divided into six layers by six circular perforated trays. Wheat bran was used as both a carrier of bound mycelia and nutrient medium for the growth ofA. awamoriandA. oryzae. The new tray SSB is equipped with instrumentation (an oxygen (O2)/carbon dioxide (CO2) gas analyser and a thermocouple) to continuously monitor O2consumption and CO2and heat evolved, which can directly be used to monitor the fungal biomass. The integrated Gompertz model was used to describe the accumulated evolution of CO2.ResultsThe results from the models strongly suggest that the evolved and accumulated CO2can be used to excellently describe fungal growth. Another important parameter that can be determined by the gas balance method is the respiratory quotient (RQ). This is the ratio of the CO2evolution rate (CER) to the O2uptake rate (OUR). The use of CER and OUR confirmed that correlated measurements of microbial activity are available, and the determination of RQ may propose an explanation for differences from expected levels. The kinetic behaviour of the fungal culture, using raw CO2, which represents an accumulation term, was integrated with respect to time and fitted to a Gompertz model, a log-like equation. The model can be used to generate parameter values that may be used to verify the experimental data, and also to simulate and optimise the process.ConclusionOverall,A. awamoriandA. oryzaehave their own ability to degrade and utilise the complex compositions contained in the solid substrate, and fermentation conditions may lead to possible comparisons. In addition, multi-stacked circular tray SSB systems demonstrated an excellent system for further investigations of mass transfer and possibly for large-scale operation, though considerable optimisation work remains to be done; for example, the height/diameter ratio and total number of trays should be optimised.
Extending harmonized national forest inventory herb layer vegetation cover observations to derive comprehensive biomass estimates
Authors: Didion Markus 
Published: March 2020

Abstract
Background
National forest inventories (NFI) have a long history providing data to obtain nationally representative and accurate estimates of growing stock. Today, in most NFIs additional data are collected to provide information on a range of forest ecosystem functions such as biodiversity, habitat, nutrient and carbon dynamics. An important driver of nutrient and C cycling is decomposing biomass produced by forest vegetation. Several studies have demonstrated that understory vegetation, particularly annual plant litter of the herb layer can contribute significantly to nutrient and C cycling in forests. A methodology to obtain comprehensive, consistent and nationally representative estimates of herb layer biomass on NFI plots could provide added value to NFIs by complementing the existing strong basis of biomass estimates of the tree and tall shrub layer. The study was based on data from the Swiss NFI since it covers a large environmental gradient, which extends its applicability to other NFIs.

Results
Based on data from 405 measurements in nine forest strata, a parsimonious model formulation was identified to predict total and non-ligneous herb layer biomass. Besides herb layer cover, elevation was the main statistically significant explanatory variable for biomass. The regression models accurately predicted biomass based on absolute percentage cover (for total biomass: R2 = 0.65, p = 0; for non-ligneous biomass: R2 = 0.76; p = 0) as well as on cover classes (R2 = 0.83; p = 0; and R2 = 0.79, p = 0), which are typically used in NFIs. The good performance was supported by the verification with data from repeated samples. For the 2nd, 3rd, and 4th Swiss NFI estimates of non-ligneous above-ground herb layer biomass 586.6 ± 7.7, 575.2 ± 7.6, and 586.7 ± 7.9 kg·ha− 1, respectively.

Conclusions
The study presents a methodology to obtain herb layer biomass estimates based on a harmonized and standardized attribute available in many NFIs. The result of this study was a parsimonious model requiring only elevation data of sample plots in addition to NFI cover estimates to provide unbiased estimates at the national scale. These qualities are particularly important as they ensure accurate, consistent, and comparable results.
Using an algorithmic approach for grouping roles and sub-units
Authors: Worren Nicolay , Christiansen Tore , Soldal Kim Verner 
Published: March 2020

AbstractA key task in organization design is to group elements (e.g., roles) into sub-units (e.g., teams or departments). This task is computationally challenging as one must take into consideration a potentially large number of interdependencies between the elements. It also requires data about work processes in the organization, which are rarely present. We have initiated a research program that aims at developing a tool—Reconfig—to improve grouping decisions. It first collects data from employees about their working relationships (i.e., interdependencies) and then uses a computer algorithm to cluster the data in the most optimal manner. The clustered solution represents the formal structure that minimizes coordination costs by grouping the most highly interdependent elements together. We describe the tool, report on two pilot applications, and discuss both the future potential and limitations of the approach.
Global distribution of entomopathogenic nematodes, Steinernema and Heterorhabditis
Authors: Bhat Aashaq Hussain , Chaubey Ashok Kumar , Askary Tarique Hassan 
Published: March 2020

AbstractEntomopathogenic nematodes (EPNs) in the families’ Steinernematidae and Heterorhabditidae are obligate insect parasites. Their easy multiplication, broad host range, compatibility with chemical pesticides, and ease in application has grabbed interest among research practitioners to work on these beneficial microorganisms. Till date, around 100 valid species ofSteinernemaand 21 species ofHeterorhabditishave been identified from different countries of the world. Extensive surveys have been conducted across the globe to isolate locally adapted EPN species and exploit them to suppress soil-dwelling and foliar insect pests in agricultural fields. Most of the new species have been described from Asia, whereas research in some Asian countries are still at infancy. Some new species have been recorded from Australia but very few surveys have been conducted in New Zealand. Likewise, less information about these tiny creatures is from Central America; however, in North America many new species have been described, some of which have been commercialized for insect pest control, whereas in South America, several native nematode species have been described and exploited as biological control agents. European countries have also been explored for EPN diversity and new species have been reported, exploited under field condition, and commercialized. Many new species and other previously described species have been reported from Africa. Despite frequent surveys in different continents of the world, number of sites touched are low and, therefore, further surveys are still needed to explore untouched geographic areas and climatic conditions, both in plantations and indigenous forests with an aim to identify and exploit additional EPN species.
Patterns and correlates of cannabis use by cumulative lifetime violence severity as target and/or perpetrator in a community sample of eastern Canadian men
Authors: O’Donnell Sue , Scott-Storey Kelly , Wuest Judith , Malcolm Jeannie , Taylor Petrea , Vincent Charlene D. 
Published: March 2020

Abstract
Background
Recent Canadian legalization of cannabis for non-medical use underscores the need to understand patterns and correlates of cannabis use among men who may be more likely than women to become problematic cannabis users. Evidence supporting an association between substance use and violence is accumulating. Current knowledge of relationships among patterns of cannabis use, violence, gender and health is limited by dichotomous measurement of cannabis use and a focus on individual types of violence rather than lifetime cumulative violence.

Methods
We collected online survey data between April 2016 and Septermber 2017 from a community convenience sample of 589 Eastern Canadian men ages 19 to 65 years and explored how socio-demographic characteristics, gender, and health varied by past-year patterns of cannabis use (i.e., daily, sometimes, never) in the total sample and by higher and lower cumulative lifetime violence severity (CLVS) measured by a 64-item CLVS scale score (1 to 4).

Results
Overall prevalence of cannabis use was 46.6% and differed significantly between lower (38.1%) and higher (55.3%) CLVS groups (χ2 (1) = 17.42, p = .000). Daily cannabis use was more likely in the higher (25.1%) than the lower group (11.9%, χ2 (2) = 31.53, p < .001). In the total sample, daily use was significantly associated with being single, less education, lower income, some gender norms, health problems, and use of other substances. Significant associations were found for sometimes cannabis use with age group 19 to 24 years, being single, some gender norms, and hazardous and binge drinking. Never use was associated with being married, more education, higher income, being older, not using other substances, and not having mental health problems. Associations between cannabis use patterns and many variables were found in both CLVS groups but effect sizes were frequently larger in the higher group.

Conclusions
These results add substantively to knowledge of relationships among lifetime cumulative violence, patterns of cannabis use, gender, socio-demographic indicators and health problems and may inform theoretical models for future testing. Additionally, findings provide critical information for the design of health promotion strategies targeted towards those most at risk in the current climate of cannabis legalization.
Managing nematodes in Egyptian citrus orchards
Authors: Abd-Elgawad Mahfouz M. M. 
Published: March 2020

AbstractBacked by its Mediterranean climate, fertile soils, cheap labor, and geographic location, Egypt ranks high among citrus producer countries. These factors can collectively offer early fruiting and long harvest season, good quality, low production costs, and closeness of export markets. However, citrus trees are attacked by many plant-parasitic nematode species in Egypt and abroad; all but one, the citrus nematodeTylenchulus semipenetrans, of which are of limited distribution.Tylenchulus semipenetransimpacts the size and quality of citrus yields differently under various conditions, but guidelines have been published to help interpret soil sample results. Other species such as migratory endoparasites (lesion nematodes), sedentary endoparasites (root-knot nematodes), and several species of ectoparasitic nematodes (spiral and stunt nematodes) can damage citrus in Egypt. Yet, due to their limited distribution and very low population densities in citrus orchards, their damaging level is uncertain or not believed to cause significant damage of citrus yield. Clearly,T.semipenetransinteracts differently with other plant pathogens often found in citrus rhizosphere. While its infection to citrus seedlings can reduce subsequent infection of roots byPhytophthora nicotianae, it can increase the virulence ofFusarium solani. Therefore, issues closely connected with citrus yield losses are presented herein. Perspectives for management of nematodes associated with citrus in Egypt were discussed in the light of their economic importance, sampling accuracy, phytosanitary measures, ecology, and biology. Cultural practices should tackle other citrus problems before managing these nematodes.
Quaternions as a solution to determining the angular kinematics of human movement
Authors: Challis John H. 
Published: March 2020

AbstractThe three-dimensional description of rigid body kinematics is a key step in many studies in biomechanics. There are several options for describing rigid body orientation including Cardan angles, Euler angles, and quaternions; the utility of quaternions will be reviewed and elaborated.The orientation of a rigid body or a joint between rigid bodies can be described by a quaternion which consists of four variables compared with Cardan or Euler angles (which require three variables). A quaternion, q = (q0, q1, q2, q3), can be considered a rotation (Ω = 2 cos−1(q0)), about an axis defined by a unit direction vector $$ \left({q}_1/\sin \left(\frac{\Omega}{2}\right),{q}_2/\sin \left(\frac{\Omega}{2}\right),{q}_3/\sin \left(\frac{\Omega}{2}\right)\right) $$q1/sinΩ2q2/sinΩ2q3/sinΩ2. The quaternion, compared with Cardan and Euler angles, does not suffer from singularities or Codman’s paradox. Three-dimensional angular kinematics are defined on the surface of a unit hypersphere which means numerical procedures for orientation averaging and interpolation must take account of the shape of this surface rather than assuming that Euclidean geometry based procedures are appropriate. Numerical simulations demonstrate the utility of quaternions for averaging three-dimensional orientations. In addition the use of quaternions for the interpolation of three-dimensional orientations, and for determining three-dimensional orientation derivatives is reviewed.The unambiguous nature of defining rigid body orientation in three-dimensions using a quaternion, and its simple averaging and interpolation gives it great utility for the kinematic analysis of human movement.
Chicken eggs, childhood stunting and environmental hygiene: an ethnographic study from the Campylobacter genomics and environmental enteric dysfunction (CAGED) project in Ethiopia
Authors: Bardosh Kevin Louis , Hussein Jeylan Wolyie , Sadik Elias Ahmed , Hassen Jemal Yousuf , Ketema Mengistu , Ibrahim Abdulmuen Mohammed , McKune Sarah Lindley , Havelaar Arie Hendrik 
Published: March 2020

Abstract
Background
Childhood stunting and malnutrition condemn millions of people globally to a life of disadvantage and cognitive and physical impairment. Though increasing egg consumption is often seen as an important solution for low and middle income countries (including Ethiopia), emerging evidence suggests that greater exposure to poultry feces may also inhibit child growth due to the effects of enteric bacteria, especially Campylobacter, on gut health.

Methods
In this rapid ethnographic study, we explored village poultry production, child dietary practices, and environmental hygiene conditions as they relate to Campylobacter risk and intervention in 16 villages in Haramaya Woreda, Eastern Ethiopia.

Results
In the study area, we found that women assumed primary responsibility to care for both chickens and children: in feeding, housing, and healthcare. Most chickens were free-range local indigenous breeds, and flock sizes were small and unstable due to epidemics, seasonal trends, reproductive patterns, and lack of food. Generally, eggs were seen as “too luxurious” to be eaten, and were predominantly sold at local markets for scarce cash, despite high malnutrition rates. Local narratives of extreme poverty, social dietary norms, parental fatalism, and lack of “dietary consciousness” (as it was called) were invoked to explain this. We found that homesteads were highly contaminated with human and animal feces. Although community members viewed chicken feces and poultry gastrointestinal contents as particularly noxious in comparison to other animals because of their feeding behaviour, they did not relate them to any particular disease. Shared human-animal housing and childcare practices place children at high risk of exposure to enteric bacteria from animal manure, despite daily routines designed to manage the domestic landscape.

Conclusions
Addressing childhood stunting and malnutrition through egg production in rural landscapes like Haramaya must navigate three distinct health and care regimes: for children, chickens, and home environments. Interventions should be based on a holistic approach to social and economic empowerment, one that considers both women and men and integrates nutrition, health, and community change as its overarching goal.
Correction to: Proposal of a new nomenclature for introns in protein-coding genes in fungal mitogenomes
Authors: Zhang Shu , Zhang Yong-Jie 
Published: March 2020

After publication of our article (Zhang and Zhang 2019) we have been notified us that the nad4L sequence should be corrected in Additional file 1. All sequences in the file should be that of NC_036382 (GenBank accession number), but have been erroneously pasted as the nad4L sequence of NC_001715.
Incidence of retromolar canal in Egyptian population using CBCT: a retrospective study
Authors: Badry Mahmoud Sayed Mahmoud , El-Badawy Fatma Mostafa , Hamed Walaa Mohamed 
Published: March 2020

Abstract
Background
Many studies have been done to investigate the incidence and the course of the retromolar canal (RMC) in different populations either osseous studies using cadavers or dry mandibles, panoramic studies, or cone beam computed tomography (CBCT) studies. The location of the RMC is in the retromolar area distal to the third mandibular molar. The retromolar area is considered an imperative site where many dental surgeries are being held.
The aim of this study is to determine the incidence of RMC in the Egyptian population using CBCT and measure the distance between the RMF and neighboring anatomical landmarks to better allocate the RMC and RMF and avoid the complications.
Two hundred and fourteen CBCT scans were examined to detect the presence of RMC. Their course was classified to 3 main types A, B, and C, and linear measurements were done from the retromolar foramen (RMF) to the 2nd and 3rd molars in anteroposterior direction and from the RMF to the mandibular foramen (MF) in anteroposterior and mediolateral directions.

Results
The RMC was present in 24 scans from the 214 with 11.2% incidence rate. The most common type was found to be type A, and type C was the rarest. The RMC has no significant difference between males and females, unilateral and bilateral distribution, and right and left sides. The linear measurements from the RMF to the 2nd and 3rd molars are 14.70 ± 5.07 mm and 4.26 ± 4.21 mm, respectively, and from the RMF to the MF in anteroposterior and mediolateral directions are 15.69 ± 3.43 mm and 2.62 ± 1.60 mm, respectively.

Conclusions
The incidence rate of RMC in Egyptian population represents 11.2%. The use of CBCT is quite important in the evaluation of the retromolar area to visualize the presence of the RMC or RMF and avoid the complications occurring due to different dental surgical procedures performed in the aforementioned site.
The interplay between childhood trauma, cognitive biases, and cannabis use on the risk of psychosis in nonclinical young adults in Poland
Authors: Frydecka Dorota , Misiak Błażej , Kotowicz Kamila , Pionke Renata , Krężołek Martyna , Cechnicki Andrzej , Gawęda Łukasz 
Published: March 2020

Abstract

Background.
Childhood traumatic events are risk factors for psychotic-like experiences (PLEs). However, the mechanisms explaining how trauma may contribute to the development of PLEs are not fully understood. In our study, we investigated whether cannabis use and cognitive biases mediate the relationship between early trauma and PLEs.

Methods.
A total sample of 6,772 young adults (age 26.6 ± 4.7, 2,181 male and 3,433 female) was recruited from the general population to participate in an online survey. We excluded 1,158 individuals due to a self-reported lifetime diagnosis of any mental disorder. The online survey included selected items from the following questionnaires: Traumatic Experience Checklist (TEC, 3 items), Childhood Experience of Care and Abuse Questionnaire (CECA.Q, 3 items), Cannabis Problems Questionnaire (CPQ, 10 items), Davos Assessment of Cognitive Biases Scale (DACOBS-18, 9 items), and Prodromal Questionnaire-16 (PQ-16). Mediation analyses were performed with respect to different categories of traumatic experiences (emotional, physical and sexual abuse as well as emotional neglect).

Results.
Our results showed significant associations of any time of childhood trauma with higher scores of cannabis use (CPQ), cognitive biases (DACOBS), and PLEs (PQ-16) (p < 0.001). We found a direct effect of childhood trauma on PLEs as well as significant indirect effect mediated through cannabis use and cognitive biases. All models tested for the effects of specific childhood adversities revealed similar results. The percentage of variance in PQ-16 scores explained by serial mediation models varied between 32.8 and 34.2% depending on childhood trauma category.

Conclusion.
Cannabis use and cognitive biases play an important mediating role in the relationship between childhood traumatic events and the development of PLEs in a nonclinical young adult population.