bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dialects, registers and intraindividual variation: Outside the scope of generative frameworks?
Authors: Eide Kristin Melum , Åfarli Tor A. 
Published: November 2020

AbstractThis article explores intraindividual microvariation in dialect syntax. We argue that in many cases the speaker has internalized a different (sub)grammar for each dialectal variety, in line with the hypothesis of universal bilingualism and parallel grammars argued for by Roeper (1999 et seq.). We discuss the question of how we can distinguish parallel grammars from optionality within one grammar, suggesting that the identification of correlating contextual factors might be a promising criterion. However, we also explore a more subtle type of variation, namely cases where a standard variety influences a potentially more vulnerable non-standard variety in a way that makes it exceedingly difficult for the language user and even for a trained linguist to discern what is what. We discuss whether or not these properties should be analysed as properties of another subgrammar (the standard grammar) or as fully integrated (albeit acquired) properties of the non-standard dialect.
Profitability prospects, risk aversion and time preferences of soybean producers in the region of Santarém, Brazilian Amazon: perspectives for an ecological transition
Authors: Leblanc Gabriel , Lucotte Marc , Mertens Frédéric , Séguin Charles 
Published: November 2020

AbstractReducing adverse environmental consequences of modern industrial agriculture requires an ecological transition of agricultural practices. An important determinant of adoption of new agricultural practices by producers is the perceived profitability of these practices. The profitability of ecological agricultural practices tends to rely on improved crop prices and reduced input use. Transition to such practices often entails increased profit volatility (risk) and long-term returns (temporal profile). Ideal candidates for transition would therefore be aware of the output price and input costs dimensions of their profitability, as well as willing to assume some risks and show patience to value long-term returns. We assessed the potential for such a transition along these three dimensions (profitability, risk aversion and time preferences) in a group of soybean producers in the agricultural frontier of the Brazilian Amazon. Primary data were collected using a questionnaire and economic tests in the region of Santarém (State of Pará, Brazil). We found that, while these producers have a low-risk aversion that could favor the adoption of new ecological practices, their focus on increasing yields to enhance profits and their high discount rates considerably reduces their propensity to adopt these practices.
Plasma Density and Magnetic Field Fluctuations in the Ion Gyro‐Frequency Range Near the Diamagnetic Cavity of Comet 67P
Authors: Odelstad E. , Eriksson A. I. , André M. , Graham D. B. , Karlsson T. , Vaivads A. , Vigren E. , Goetz C. , Nilsson H. , Henri P. , Stenberg‐Wieser G. 
Published: November 2020

AbstractWe report the detection of large‐amplitude, quasi‐harmonic density fluctuations with associated magnetic field oscillations in the region surrounding the diamagnetic cavity of comet 67P. Typical frequencies are ∼0.1 Hz, corresponding to ∼10 times the water and
times the proton gyro‐frequencies, respectively. Magnetic field oscillations are not always clearly observed in association with these density fluctuations, but when they are, they consistently have wave vectors perpendicular to the background magnetic field, with the principal axis of polarization close to field‐aligned and with a ∼90° phase shift with respect to the density fluctuations. The fluctuations are observed in association with asymmetric plasma density and magnetic field enhancements previously found in the region surrounding the diamagnetic cavity, occurring predominantly on their descending slopes. This is a new type of waves not previously observed at comets. They are likely ion Bernstein waves, and we propose that they are excited by unstable ring, ring‐beam, or spherical shell distributions of cometary ions just outside the cavity boundary. These waves may play an important role in redistributing energy between different particle populations and reshape the plasma environment of the comet.
Hail Climatology Along the Northeastern Adriatic
Authors: Jelić Damjan , Megyeri Otília Anna , Malečić Barbara , Belušić Vozila Andreina , Strelec Mahović Nataša , Telišman Prtenjak Maja 
Published: November 2020

AbstractGeneral awareness and overall interest regarding hailstorms and hail properties in Europe have increased significantly in the last several decades and have resulted in numerous local, national, and even Europe‐wide studies on hail and hail properties. To contribute to this field, we determined the hail climatology in the northeastern (NE) Adriatic region and analyzed its spatial and temporal patterns and performed an objectively derived weather type analysis of ERA5 daily mean data and instability indices. We studied the NE Adriatic region due to its focus on agricultural activities and on quality wine production. Our results are based on approximately 60 years of high spatial resolution measurements collected from 27 stations across complex terrain. The results show (i) high levels of spatial variability, (ii) significant annual variations, and (iii) hail throughout the whole year that (iv) intensifies in summer months. Furthermore, redistribution of hail among seasons (in particular, from summer to spring) was detected. Most significant changes were visible in the June–October period, with a negative trend of −0.06 hail days/year, and the period from November to March exhibited a positive trend of 0.13 hail cases/year. We found that deep cyclonic systems in front of and above our domain were most responsible for hail generation, often supported by southwesterly winds. Additionally, the vast majority of observed hail events occurred in unstable and sheared environments.
Mutational profiles associated with resistance in patients with BRAFV600E mutant colorectal cancer treated with cetuximab and encorafenib +/− binimetinib or alpelisib
Authors: Huijberts Sanne C. F. A. , Boelens Mirjam C. , Bernards Rene , Opdam Frans L. 
Published: November 2020

Abstract
Background
Treatment strategies inhibiting BRAF in combination with EGFR have been developed in patients with BRAFV600E mutant metastatic colorectal cancer, but intrinsic and secondary resistance remains a challenge. We aimed to investigate which genetic alterations cause intrinsic non-response and/or acquired resistance in these patients receiving therapies consisting of a backbone of BRAF and EGFR inhibition.

Methods
This was a cohort study on genetic alterations in patients with BRAFV600E mutant advanced colorectal cancer treated with inhibitors of the MAPK pathway. We examined tumour tissue for genetic alterations at baseline, during treatment and at progression.

Results
In total, 37 patients were included in this cohort. Genetic alterations in EGFR and in PIK3CA are associated with non-response. A greater fraction of non-responders (75%) versus responders (46%) had at least one genetic alteration in other genes than TP53, APC or BRAF. Secondary resistance mutations (n = 16 patients) were observed most frequently in the PI3K pathway (n = 6) and in receptor tyrosine kinases (n = 4), leading to increased upstream signalling.

Conclusions
Genetic alterations in the PI3K and upstream receptor tyrosine kinases were mostly associated with intrinsic and acquired resistance. By understanding these alterations, simultaneous or alternating treatments with targeted inhibitors might improve response duration.
Mutational drivers of cancer cell migration and invasion
Authors: Novikov Nikita M. , Zolotaryova Sofia Y. , Gautreau Alexis M. , Denisov Evgeny V. 
Published: November 2020

AbstractGenomic instability and mutations underlie the hallmarks of cancer—genetic alterations determine cancer cell fate by affecting cell proliferation, apoptosis and immune response, and increasing data show that mutations are involved in metastasis, a crucial event in cancer progression and a life-threatening problem in cancer patients. Invasion is the first step in the metastatic cascade, when tumour cells acquire the ability to move, penetrate into the surrounding tissue and enter lymphatic and blood vessels in order to disseminate. A role for genetic alterations in invasion is not universally accepted, with sceptics arguing that cellular motility is related only to external factors such as hypoxia, chemoattractants and the rigidity of the extracellular matrix. However, increasing evidence shows that mutations might trigger and accelerate the migration and invasion of different types of cancer cells. In this review, we summarise data from published literature on the effect of chromosomal instability and genetic mutations on cancer cell migration and invasion.
SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography
Authors: Klein Steffen , Cortese Mirko , Winter Sophie L. , Wachsmuth-Melm Moritz , Neufeldt Christopher J. , Cerikan Berati , Stanifer Megan L. , Boulant Steeve , Bartenschlager Ralf , Chlanda Petr 
Published: November 2020

AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the COVID19 pandemic, is a highly pathogenic β-coronavirus. As other coronaviruses, SARS-CoV-2 is enveloped, replicates in the cytoplasm and assembles at intracellular membranes. Here, we structurally characterize the viral replication compartment and report critical insights into the budding mechanism of the virus, and the structure of extracellular virions close to their native state by in situ cryo-electron tomography and subtomogram averaging. We directly visualize RNA filaments inside the double membrane vesicles, compartments associated with viral replication. The RNA filaments show a diameter consistent with double-stranded RNA and frequent branching likely representing RNA secondary structures. We report that assembled S trimers in lumenal cisternae do not alone induce membrane bending but laterally reorganize on the envelope during virion assembly. The viral ribonucleoprotein complexes (vRNPs) are accumulated at the curved membrane characteristic for budding sites suggesting that vRNP recruitment is enhanced by membrane curvature. Subtomogram averaging shows that vRNPs are distinct cylindrical assemblies. We propose that the genome is packaged around multiple separate vRNP complexes, thereby allowing incorporation of the unusually large coronavirus genome into the virion while maintaining high steric flexibility between the vRNPs.
X-ray flares from the stellar tidal disruption by a candidate supermassive black hole binary
Authors: Shu Xinwen , Zhang Wenjie , Li Shuo , Jiang Ning , Dou Liming , Yan Zhen , Xie Fu-Guo , Shen Rongfeng , Sun Luming , Liu Fukun , Wang Tinggui 
Published: November 2020

AbstractOptical transient surveys have led to the discovery of dozens of stellar tidal disruption events (TDEs) by massive black hole in the centers of galaxies. Despite extensive searches, X-ray follow-up observations have produced no or only weak X-ray detections in most of them. Here we report the discovery of delayed X-ray brightening around 140 days after the optical outburst in the TDE OGLE16aaa, followed by several flux dips during the decay phase. These properties are unusual for standard TDEs and could be explained by the presence of supermassive black hole binary or patchy obscuration. In either scenario, the X-rays can be produced promptly after the disruption but are blocked in the early phase, possibly by a radiation-dominated ejecta which leads to the bulk of optical and ultraviolet emission. Our findings imply that the reprocessing is important in the TDE early evolution, and X-ray observations are promising in revealing supermassive black hole binaries.
Phased-array combination of 2D MRS for lipid composition quantification in patients with breast cancer
Authors: Mallikourti Vasiliki , Cheung Sai Man , Gagliardi Tanja , Senn Nicholas , Masannat Yazan , McGoldrick Trevor , Sharma Ravi , Heys Steven D. , He Jiabao 
Published: November 2020

AbstractLipid composition in breast cancer, a central marker of disease progression, can be non-invasively quantified using 2D MRS method of double quantum filtered correlation spectroscopy (DQF-COSY). The low signal to noise ratio (SNR), arising from signal retention of only 25% and depleted lipids within tumour, demands improvement approaches beyond signal averaging for clinically viable applications. We therefore adapted and examined combination algorithms, designed for 1D MRS, for 2D MRS with both internal and external references. Lipid composition spectra were acquired from 17 breast tumour specimens, 15 healthy female volunteers and 25 patients with breast cancer on a clinical 3 T MRI scanner. Whitened singular value decomposition (WSVD) with internal reference yielded maximal SNR with an improvement of 53.3% (40.3–106.9%) in specimens, 84.4 ± 40.6% in volunteers, 96.9 ± 54.2% in peritumoural adipose tissue and 52.4% (25.1–108.0%) in tumours in vivo. Non-uniformity, as variance of improvement across peaks, was low at 21.1% (13.7–28.1%) in specimens, 5.5% (4.2–7.2%) in volunteers, 6.1% (5.0–9.0%) in peritumoural tissue, and 20.7% (17.4–31.7%) in tumours in vivo. The bias (slope) in improvement ranged from − 1.08 to 0.21%/ppm along the diagonal directions. WSVD is therefore the optimal algorithm for lipid composition spectra with highest SNR uniformly across peaks, reducing acquisition time by up to 70% in patients, enabling clinical applications.
The relationship between specialized pro-resolving lipid mediators, morbid obesity and weight loss after bariatric surgery
Authors: Schulte Fabian , Asbeutah Abdul Aziz , Benotti Peter N. , Wood G. Craig , Still Christopher , Bistrian Bruce R. , Hardt Markus , Welty Francine K. 
Published: November 2020

AbstractObesity and diabetes are associated with chronic inflammation. Specialized pro-resolving lipid mediators (SPMs)—resolvins (Rv), protectins (PD) and maresins (MaR)—actively resolve inflammation. Bariatric surgery achieves remission of diabetes, but mechanisms are unclear. We measured SPMs and proinflammatory eicosanoid levels using liquid chromatography-tandem mass spectrometry in 29 morbidly obese subjects (13 with diabetes) and 15 nondiabetic, mildly obese subjects. Compared to the mildly obese, the morbidly obese had higher levels of SPMs—RvD3, RvD4 and PD1—and white blood cells (WBC) and platelets. Post-surgery, SPM and platelet levels decreased in morbidly obese nondiabetic subjects but not in diabetic subjects, suggesting continued inflammation. Despite similar weight reductions 1 year after surgery (44.6% vs. 46.6%), 8 diabetes remitters had significant reductions in WBC and platelet counts whereas five non-remitters did not. Remitters had a 58.2% decrease (p = 0.03) in 14-HDHA, a maresin pathway marker; non-remitters had an 875.7% increase in 14-HDHA but a 36.9% decrease in MaR1 to a median of 0. In conclusion, higher levels of RvD3, PD1 and their pathway marker, 17-HDHA, are markers of leukocyte activation and inflammation in morbid obesity and diabetes and diminish with weight loss in nondiabetic but not diabetic subjects, possibly representing sustained inflammation in the latter. Lack of diabetes remission after surgically-induced weight loss may be associated with reduced ability to produce MaR1 and sustained inflammation.
Genome-wide association study to identify genomic regions and positional candidate genes associated with male fertility in beef cattle
Authors: Sweett H. , Fonseca P. A. S. , Suárez-Vega A. , Livernois A. , Miglior F. , Cánovas A. 
Published: November 2020

AbstractFertility plays a key role in the success of calf production, but there is evidence that reproductive efficiency in beef cattle has decreased during the past half-century worldwide. Therefore, identifying animals with superior fertility could significantly impact cow-calf production efficiency. The objective of this research was to identify candidate regions affecting bull fertility in beef cattle and positional candidate genes annotated within these regions. A GWAS using a weighted single-step genomic BLUP approach was performed on 265 crossbred beef bulls to identify markers associated with scrotal circumference (SC) and sperm motility (SM). Eight windows containing 32 positional candidate genes and five windows containing 28 positional candidate genes explained more than 1% of the genetic variance for SC and SM, respectively. These windows were selected to perform gene annotation, QTL enrichment, and functional analyses. Functional candidate gene prioritization analysis revealed 14 prioritized candidate genes for SC of whichMAP3K1andVIPwere previously found to play roles in male fertility. A different set of 14 prioritized genes were identified for SM and five were previously identified as regulators of male fertility (SOD2, TCP1, PACRG, SPEF2, PRLR). Significant enrichment results were identified for fertility and body conformation QTLs within the candidate windows. Gene ontology enrichment analysis including biological processes, molecular functions, and cellular components revealed significant GO terms associated with male fertility. The identification of these regions contributes to a better understanding of fertility associated traits and facilitates the discovery of positional candidate genes for future investigation of causal mutations and their implications.
Artificial intelligence for the detection of vertebral fractures on plain spinal radiography
Authors: Murata Kazuma , Endo Kenji , Aihara Takato , Suzuki Hidekazu , Sawaji Yasunobu , Matsuoka Yuji , Nishimura Hirosuke , Takamatsu Taichiro , Konishi Takamitsu , Maekawa Asato , Yamauchi Hideya , Kanazawa Kei , Endo Hiroo , Tsuji Hanako , Inoue Shigeru , Fukushima Noritoshi , Kikuchi Hiroyuki , Sato Hiroki , Yamamoto Kengo 
Published: November 2020

AbstractVertebral fractures (VFs) cause serious problems, such as substantial functional loss and a high mortality rate, and a delayed diagnosis may further worsen the prognosis. Plain thoracolumbar radiography (PTLR) is an essential method for the evaluation of VFs. Therefore, minimizing the diagnostic errors of VFs on PTLR is crucial. Image identification based on a deep convolutional neural network (DCNN) has been recognized to be potentially effective as a diagnostic strategy; however, the accuracy for detecting VFs has not been fully investigated. A DCNN was trained with PTLR images of 300 patients (150 patients with and 150 without VFs). The accuracy, sensitivity, and specificity of diagnosis of the model were calculated and compared with those of orthopedic residents, orthopedic surgeons, and spine surgeons. The DCNN achieved accuracy, sensitivity, and specificity rates of 86.0% [95% confidence interval (CI) 82.0–90.0%], 84.7% (95% CI 78.8–90.5%), and 87.3% (95% CI 81.9–92.7%), respectively. Both the accuracy and sensitivity of the model were suggested to be noninferior to those of orthopedic surgeons. The DCNN can assist clinicians in the early identification of VFs and in managing patients, to prevent further invasive interventions and a decreased quality of life.
Monitoring atmospheric particulate matters using vertically resolved measurements of a polarization lidar, in-situ recordings and satellite data over Tehran, Iran
Authors: Panahifar Hossein , Moradhaseli Ruhollah , Khalesifard Hamid Reza 
Published: November 2020

AbstractThe highly polluted atmosphere above Tehran has been investigated by using a polarization lidar operating at 532 nm, in-situ particulate matter suites distributed over the city, and meteorological observations. The measurement campaign is conducted from Nov. 2014 to Jan. 2016. Three typical cases are studied in detail where, the atmosphere is polluted with urban pollution, mixture of urban pollution and dust particles from local sources, and long range transported dust from the Arabian Peninsula. For these cases, vertical profiles of the lidar backscatter coefficient, extinction coefficients, particle depolarization ratio ($$\delta _{\text {p}}$$

δ
p

) and mass concentrations of atmospheric aerosols (separated into dust and non-dust particles) are presented. Using the lidar recordings, variations of the planetary boundary layer height above the city are investigated along the year. During November to February, lidar profiles frequently show polluted boundary layers that are reaching up to 1 km above the ground level. The depolarization ratio ($$\delta _{\text {p}}$$

δ
p

) varies between 0.04 and 0.08 in the polluted boundary layer. During the campaign, for 103 days the urban pollution was dominant, 45 recorded dust events ($$0.15<\delta _{\text {p}}<0.20$$

0.15
<

δ
p

<
0.20

) were originated from the dry regions in the south of Tehran and 15 dust events ($$0.20<\delta _{\text {p}}<0.35$$

0.20
<

δ
p

<
0.35

) impacted the city that were originated from the Arabian Desert and Mesopotamia.
Insights for the application of TILs and AR in the treatment of TNBC in routine clinical practice
Authors: Losurdo Agnese , De Sanctis Rita , Fernandes Bethania , Torrisi Rosalba , Masci Giovanna , Agostinetto Elisa , Gatzemeier Wolfgang , Errico Valentina , Testori Alberto , Tinterri Corrado , Roncalli Massimo , Santoro Armando 
Published: November 2020

AbstractTriple negative breast cancer (TNBC), usually presenting with a very aggressive phenotype, is a heterogeneous entity. We aim to discuss new biomarkers, suitable for prognostic and predictive purposes. We retrospectively collected clinical variables and immunohistochemical characteristics of early TNBCs, specifically focusing on the prognostic and predictive significance of tumor infiltrating lymphocytes (TILs) and androgen receptor (AR) expression, assessing their correlation with clinical variables. Among 159 patients, TILs were significantly higher in younger patients and with lower BMI, and in tumors with higher ki-67 and greater nodal involvement; conversely, AR was significantly higher in older patients and in tumors with lower ki-67. Interestingly and in line with literature, both TILs level and ARs expression were lower within metastatic sites, in patients who developed distant metastases, compared to those found in the primary site. Small (pT1) and node negative tumors were highly represented and no correlation of either TILs or AR with prognosis could be observed. Our findings support the use of stromal TILs to identify a more aggressive, but chemo-sensitive phenotype, mostly represented in younger women, while AR may identify a less aggressive, slow-growing luminal TNBC subtype, more common among older patients. TILs and AR are worth implementing in routine clinical practice to refine prognosis even if, in our case series, we couldn’t identify a significant correlation of the two variables with either disease-free and overall survival.