bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overdiagnosis of melanoma – causes, consequences and solutions
Authors: Kutzner Heinz , Jutzi Tanja B. , Krahl Dieter , Krieghoff‐Henning Eva I. , Heppt Markus V. , Hekler Achim , Schmitt Max , Maron Roman C. R. , Fröhling Stefan , von Kalle Christof , Brinker Titus J. 
Published: August 2020

SummaryMalignant melanoma is the skin tumor that causes most deaths in Germany. At an early stage, melanoma is well treatable, so early detection is essential. However, the skin cancer screening program in Germany has been criticized because although melanomas have been diagnosed more frequently since introduction of the program, the mortality from malignant melanoma has not decreased. This indicates that the observed increase in melanoma diagnoses be due to overdiagnosis, i.e. to the detection of lesions that would never have created serious health problems for the patients. One of the reasons is the challenging distinction between some benign and malignant lesions. In addition, there may be lesions that are biologically equivocal, and other lesions that are classified as malignant according to current criteria, but that grow so slowly that they would never have posed a threat to patient’s life. So far, these “indolent” melanomas cannot be identified reliably due to a lack of biomarkers. Moreover, the likelihood that an in‐situ melanoma will progress to an invasive tumor still cannot be determined with any certainty. When benign lesions are diagnosed as melanoma, the consequences are unnecessary psychological and physical stress for the affected patients and incurred therapy costs. Vice versa, underdiagnoses in the sense of overlooked melanomas can adversely affect patients’ prognoses and may necessitate more intense therapies. Novel diagnostic options could reduce the number of over‐ and underdiagnoses and contribute to more objective diagnoses in borderline cases. One strategy that has yielded promising results in pilot studies is the use of artificial intelligence‐based diagnostic tools. However, these applications still await translation into clinical and pathological routine.
Are juveniles as tolerant to salinity stress as adults? A case study of Northern European, Ponto‐Caspian and North American species
Authors: Paiva Filipa , Pauli Nora‐Charlotte , Briski Elizabeta 
Published: August 2020

AbstractAimGlobal biodiversity and ecosystems are highly impacted by anthropogenic activities, such as climate change and introduction of non‐indigenous species. As numerous species from the Ponto‐Caspian region have established in the North and Baltic Seas, as well as in the Laurentian Great Lakes, there have been large number of studies examining environmental tolerance of these species to determine their future potential to spread. However, many of those studies were conducted only on adult stages, while neglecting the possibility that early life history stages might not be equally resilient.LocationNorthern European, Ponto‐Caspian and North American regions.MethodsTo determine whether juveniles would demonstrate the same environmental tolerance as their parents, we examined the salinity tolerance of adults and juveniles of one Northern European (Gammarus salinus), one Ponto‐Caspian (Pontogammarus maeoticus) and one North American species (Gammarus tigrinus). Additionally, we compared our study to that of Paiva et al. (Global Change Biology, 24, 2018, 2708), who tested the salinity tolerance of the same species using only adults.ResultsOur study determined that both adults and juveniles of all three species tolerated wide ranges of salinity, with juveniles of G. salinus tolerating only slightly narrower salinity range than their parents, while those of P. maeoticus and G. tigrinus much narrower range. Additionally, we determined better survival and higher growth rates of juveniles of G. salinus in higher salinities and better survival of P. maeoticus in lower salinities.Main conclusionsBased on juvenile salinity tolerance, our study further supported findings of Paiva et al. (2018), where Northern European species may be adapted to marine, while Ponto‐Caspian to lower saline and freshwater environments. The North American species is probably adapted to intermediate salinities. As juveniles do not tolerate the same salinity stress as adults, we emphasize the importance of testing all life history stages when predicting species resilience to environmental stressors.
Deep brain stimulation in Parkinson’s disease patients and routine 6‐OHDA rodent models: Synergies and pitfalls
Authors: Stefani Alessandro , Cerroni Rocco , Pierantozzi Mariangela , D’Angelo Vincenza , Grandi Laura , Spanetta Matteo , Galati Salvatore 
Published: August 2020

AbstractThe history of deep brain stimulation for Parkinson's disease (PD) represented a paradigmatic cross‐talk between mammalian disease models and clinical evidence in humans. Fascinating were the results achieved by high frequency stimulation (HFS) into the subthalamic nucleus (STN) of MPTP‐treated primates. An analogous strategy relieved tremor and hypokinetic parameters in PD patients. The 6‐hydroxydopamine (6‐OHDA) rodent model has mastered decades of research, contributing to understanding of the PD pathology. However, this review wonders about the actual synergy between the routine neurotoxic models and PD patients underlying STN‐DBS. At first, some findings collected following 6‐OHDA, promoted dogmatic visions, as the wrong contention that suppression of STN glutamate was the key therapeutic player. Instead, changes of glutamate release are negligible in humans during transition to ON‐state. Besides, the imbalance of basal ganglia endogenous band frequencies, the beta (β) band increase and the cortical‐basal ganglia synchronization, undisputedly shared by models and PD patients, do not govern the whole spectrum of non‐motor PD signs, difficult to investigate in rodents. Furthermore, the tonic release of dopamine, inferred during HFS in rodents, was not replicated in humans. Finally, neurotoxic rodent models describe a ‘pure’ dopamine depletion sparing pathways crucial in parkinsonian phenotypes, that is, noradrenergic and cholinergic ones. Although the utilization of neurotoxic models is still providing major advancements, we pore over these contradictions and try to support possible amendments of neurotoxic models (advocating modern ‘in vivo’ approaches and recordings extending towards motor thalamus) for pursing the development of new DBS technology.
NBAS disease: 14 new patients, a recurrent mutation, and genotype–phenotype correlation among 24 Chinese patients
Authors: Li Zhong Die , Abuduxikuer Kuerbanjiang , Zhang Jing , Yang Ye , Qiu Yi‐Ling , Huang Yuge , Xie Xin‐Bao , Lu Yi , Wang Jian‐She 
Published: August 2020

AimNeuroblastoma amplified sequence (NBAS)‐associated disease has a wide phenotypic spectrum, including infantile liver failure syndrome type 2 (ILFS2, OMIM #616483), short stature with optic nerve atrophy and Pelger–Huët anomaly (SOPH) syndrome (OMIM #614800), and a combined phenotype overlapping ILFS2 and SOPH syndrome. The mutation spectra of NBAS and its genotype–phenotype correlation among Chinese were not clear.MethodsClinical and genetic data were retrospectively collected from the medical charts of patients with biallelic NBAS mutations, as well as from Chinese patients in previously published reports.ResultsFourteen new patients were identified, including 10 novel mutations: c.648‐1G>A, c.2563_c.2577+5del/p.His855_Gln859del, c.3115C>T/p.Gln1039Ter, c.3284G>A/p.Trp1095Ter, c.2570C>T/p.Ala857Val, c.6859G>T/p.Asp2287Tyr, c.1028G>A/p.Ser343Asn, c.1177_1182delinsAGATAGA/p.Val393ArgfsTer2, c.3432_3435dupCAGT/p.Ala1146GlnfsTer14, and c.680_690dupACTGTTTCAGC/p.Phe231ThrfsTer35. All 14 patients presented as fever‐triggered liver injury, including nine patients that satisfied the criteria of acute liver failure (ALF) in whom c.3596G>A/p.Cys1199Tyr occurred five times. Nine patients had extrahepatic manifestations including short stature, skeletal abnormalities, intellectual disability, ophthalmic abnormalities, low levels of serum immunoglobulins, facial dysmorphism, and cardiac abnormalities. Ten other Chinese patients were collected through a review of published works. Genotype–phenotype analysis in 24 Chinese patients revealed that the percentage of ALF patients with variants in the Sec39 domain was significantly higher than that in the C‐terminal (100% vs. 12.5%, P = 0.000), and the percentage of multi‐organ/system involvement in patients with variants in the Sec39 domain was significantly lower than that in the C‐terminal (40% vs. 100%, P = 0.0128).ConclusionsWe reported 14 new patients, 10 novel mutations, and a unique recurrent mutation. Correlation analysis indicated that the domain of missense and non‐frameshift insertion/deletion mutations in NBAS protein is related to phenotype among Chinese patients.
Percutaneous nephrostomy versus JJ ureteric stent as the initial drainage method in kidney stone patients presenting with acute kidney injury: A prospective randomized study
Authors: Elbatanouny Ahmed M , Ragheb Ahmed M , Abdelbary Ahmed M , Fathy Hany , Massoud Amr M , Abd El Latif Ahmed , Moussa Ayman S , Ibrahim Rabie M 
Published: August 2020

ObjectiveTo compare percutaneous nephrostomy tube versus JJ stent as an initial urinary drainage procedure in kidney stone patients presenting with acute kidney injury.MethodsBetween January 2017 and January 2019, 143 patients with acute kidney injury secondary to obstructive kidney stone were prospectively randomized into the percutaneous nephrostomy tube group (71 patients) and JJ stent group (72 patients) at Beni‐Suef University Hospital, Beni‐Suef, Egypt. Exclusion criteria included candidates for acute dialysis, fever (>38°C), pyonephrosis, pregnancy and uncontrolled coagulopathy. The period required for serum creatinine normalization, failure of insertion, operative and fluoroscopy time were recorded. Definitive stone management for proximal ureteral stones >1.5 cm consisted of percutaneous nephrolithotomy for the percutaneous nephrostomy group and ureteroscopic laser lithotripsy for the JJ stent group. For stone size <1.5 cm, ureteroscopy or shockwave lithotripsy was carried out for both groups. Percutaneous nephrolithotomy was carried out for renal stones >2 cm, and shockwave lithotripsy for stones <2 cm. Distal and mid ureteral stones were treated by ureteroscopy.ResultsThe percutaneous nephrostomy group had shorter operative time (P = 0.001). There was no significant difference in the recovery period for normalization of serum creatinine between both groups (P = 0.120). Procedural failure, ureteric mucosal injury and perforations increased in the case of male sex, stone size >1.5 cm and upper ureteric stones in the JJ stent group. Procedural failure, pelvic perforations and intraoperative bleeding increased in case of male sex, mild hydronephrosis and stone size >2.5 cm in the percutaneous nephrostomy group. Suprapubic pain, urethral pain and lower urinary tract symptoms were significant in the JJ stent group. The presence of a JJ stent directed us toward ureteroscopy (P = 0.002) and the presence of a percutaneous nephrostomy directed us toward percutaneous nephrolithotomy (P = 0.001).ConclusionsPercutaneous nephrostomy facilitates subsequent percutaneous nephrolithotomy, especially when carried out by a urologist, and it has a higher insertion success rate, a shorter operative time and a lesser incidence of postoperative urinary tract infection than a JJ stent. A JJ stent facilitates subsequent ureteroscopy, but operative complications can increase in the case of proximal ureteral stones >1.5 cm.
Role of hospital anxiety and depression on the healing of chronic leg ulcer: A prospective study
Authors: Kumar Navin , Huda Farhanul , Mani Rishit , Singla Tanuj , Kundal Ashikesh , Sharma Jyoti , Gajula Bhargav 
Published: August 2020

AbstractA chronic leg ulcer is a debilitating illness, owing to the local condition of the wound and a decrease in physical activity and productivity, resulting in anxiety and depression among patients. The study aimed to find any association of anxiety and depression in the healing of chronic wounds. A total of 125 patients with chronic leg ulcers were enrolled in the study. HADS questionnaire assessment followed the local wound examination in all patients. A follow‐up assessment of the ulcer was done after appropriate local treatment, and data analysed with the HADS scale. In the present study, the ROC curve showed a cutoff value of 14 for the HADS score in predicting ulcer status (non‐healing vs healed) after 30 days. A total of 54.4% (68) patients had a HADS score ≥ 14 and 39% (49) were true positive for the non‐healing wound at a 1‐month follow‐up. This study revealed a sensitivity and specificity of 83.1% and 71.2%, respectively (P‐value <.001), and diagnostic accuracy of 76.8%, for HADS score > 14 in the detection of non‐healing ulcers. Chronic leg ulcers should be subjected to HADS assessment and if found significant corrective measures must be instituted for improving wound healing.
Skeletal Muscle Fibres and Muscle Enzyme Activities in Monozygous and Dizygous Twins of Both Sexes
Authors: Komi P. V. , Viitasalo J. H. T. , Havu M. , Thorstensson A. , Sjödin B. , Karlsson J. 
Published: August 2020

AbstractKomi, P. V., J. H. T. Viitasalo, M. Havu, A. Thorstensson, B. Sjödin and J. Karlsson. Skeletal muscle fibres and muscle enzyme activities in monozygous and dizygous twins of both sexes. Acta physiol. scand. 1977. 100. 385‐392.Significance of the genetic component in determining the interindividual variation observed in skeletal muscle fibre composition and enzyme activities was investigated in 31 pairs of male and female monozygous (MZ) and dizygous (DZ) twins, whose ages ranged in all but one pair (11 years) from 15 to 24 years. Percent distribution of slow twitch muscle fibres and activities of Ca2+ and Ng2+ stimulated ATPases, creatine phosphokinase, myokinase, phosphorylase, lactate dehydrogenase (LDH) and distribution of its isozyme LDH‐1 were all analyzed in biopsy samples taken from the vastus lateralis muscle. The data disclosed that in contrast to DZ twins the MZ twins of both sexes had an essentially identical muscle fibre composition. Calculation of the heritability estimate for this parameter gave the values of 99.5% and 92.8%, respectively for males and females. In contrast to the fibre composition presence of a significant genetic component was not observed in any of the enzyme activities studied. It was concluded that there is a predominant genetic influence on the skeletal muscle fibre composition in man, and thus also on the potential capacity of the muscles to perform work.
Brg1 regulates murine liver regeneration by targeting miR‐187‐5p dependent on Hippo signalling pathway
Authors: Gong Junhua , Mou Tong , Wu Hao , Wu Zhongjun 
Published: August 2020

AbstractBrg1 and Hippo signalling pathway are abnormally expressed in many malignant tumours, especially in Hepatocellular carcinoma, but their role in liver regeneration (LR) is unknown. In our research, we investigated the role of Brg1 and Hippo signalling pathway in hepatocyte proliferation and LR. Following 2/3 partial hepatectomy (PH) in liver‐specific Brg1 deleted mice (Brg1−/−) (KO) mice and sex‐matched wild‐type (WT), depletion of Brg1 in mouse embryos caused liver cell growth disorders and significantly decreased expression of miR‐187‐5p. We identified LATS1 as a target gene of miR‐187‐5p and the introduction of miR‐187‐5p decrease the expression of LATS1 and inactivated the Hippo signalling pathway, which facilitated the expression of cell cycle‐related proteins, and rescues the inhibitory effect of Brg1 in LR. Taken together, our findings suggested that deletion of Brg1 inhibits hepatocyte proliferation and LR by targeting miR‐187‐5p dependent on Hippo signalling pathway.
A review of the genus Strophidon (Anguilliformes: Muraenidae), with description of a new species
Authors: Huang Wen‐Chien , Mohapatra Anil , Thu Pham The , Chen Hong‐Ming , Liao Te‐Yu 
Published: August 2020

AbstractStrophidon McClelland is a muraenid genus with characteristic appearance of a very elongated body, a large mouth cleft and anteriorly placed eyes. The nomenclature and taxonomic history of species within Strophidon are contentious and its members are easily misidentified. In the present study, species of the genus Strophidon are revised based on morphological and molecular data, and five species are considered valid, including S. dawydoffi Prokofiev, S. dorsalis (Seale), S. sathete (Hamilton), S. ui Tanaka and a new species, S. tetraporus. Strophidon tetraporus sp. nov. is described based on 15 specimens from Indonesia, the Philippines, Taiwan and Vietnam with the unique characteristic of the constant presence of the fourth infraorbital pore among species of Strophidon. The intraspecific variation of vertebral formula within S. dorsalis is discussed based on molecular data. Muraena macrurus Bleeker and Thyrsoidea longissima Kaup are synonyms of S. sathete that can be distinguished from the most similar congener S. ui by a longer tail, smaller eyes and more inner maxillary and inner dentary teeth. A key to identify species of Strophidon is provided. The distribution and maximum size of each species are also re‐evaluated.
Minimizing endoscopist facial exposure to droplets: Optimal patient‐endoscopist distance and use of a barrier device
Authors: Suzuki Sho , Gotoda Takuji , Ikehara Hisatomo , Ichijima Ryoji , Kusano Chika 
Published: August 2020

AbstractBackground and AimMinimizing endoscopist exposure to bodily fluids is important for reducing the risk of infection transmission. This study investigated the patient‐endoscopist vertical distance necessary to minimize an endoscopist's facial exposure to a patient's visible droplets during upper gastrointestinal endoscopy and the ability of a new device to prevent droplets from reaching the endoscopist's face.MethodsA model was developed to simulate a patient experiencing a forceful cough during an upper gastrointestinal endoscopy with a model endoscopist. Fluorescent dye was expelled from the model patient's mouth towards the model endoscopist during simulated coughs; dye adhesion to the model endoscopist's face was evaluated using ultraviolet light. The simulation was repeated with the model patient positioned 70–100 cm above the floor, with and without a barrier to shield the patient's face. The accuracy of the cough simulation model and the relationship between patient‐endoscopist vertical distance and endoscopist's facial exposure were evaluated.ResultsThe flow dynamics of the cough simulation model were similar to that of an actual human cough. There was a significant inverse correlation between the patient‐endoscopist vertical distance and the model endoscopist's facial exposure, with positive exposures decreasing from 87% at 70 cm to 0% at 100 cm (P < 0.001). The barrier device prevented facial exposure to droplets at all distances.ConclusionsWe found that positioning the patient at least 100 cm below the top of the endoscopist's head or using a barrier device minimized the endoscopist's facial exposure to visible droplets during upper gastrointestinal endoscopy.
Effect of newborn bathing training with the swaddled and tub bathing methods given to primiparous pregnant women on the mother's experience, satisfaction and newborn's stress during the first bathing of the newborn at home: A mixed method study
Authors: Çınar Nursan , Yalnızoğlu Çaka Sinem , Uslu Yuvacı Hilal 
Published: August 2020

AbstractAimThe aim of this study is to compare the effect of two different bathing methods training given during pregnancy on the mother's experience, satisfaction and newborn's physiological and behavioral parameters during the first bathing of the newborn at home.MethodsA mixed methods research was used in this study. The groups were determined as swaddled bathing (SB) (n = 31) and tub bathing (TB) (n = 31). After training given to the groups, the first bath of the newborn in both groups was built by the mothers. The body temperature, heart rate, oxygen saturation, respiration rate, crying times, stress parameters of the newborns and mother satisfaction in both groups were evaluated. The open‐ended questions were used to determine mothers' experiences.ResultsThere was a statistically significant difference between the groups in terms of oxygen saturation, respiration rate, crying time, face/forehead grimacing, unrest and maternal satisfaction. Four categories and 14 subcategories made up the qualitative part of the study which includes the experiences of the mother about two different bathing methods. “The feelings and worries of the mothers about first bathing” refers to the participants in both groups how they feel during the first bathing; “Positive aspects of this method compared to other bath method” and “Negative aspects of this method compared to other bath method” refers to positive and negative aspects of different bathing methods applied to groups; “Difference between the applied bathing method and other methods” refers to differences they observed when compared to other bathing methods. The mothers in the SB group had less fear of drowning hazard and injuring the newborn, and the fear of uneasiness and the newborn slipping down from hands were more frequent in the TB group.ConclusionIt was determined that both bath methods reduced body temperature, the SB method decreased the stress of newborns and had a positive effect on mother satisfaction and experience.
Recommendations for the treatment of tear trough deformity with cross‐linked hyaluronic acid filler
Authors: Anido Javier , Fernández José Manuel , Genol Ignacio , Ribé Natalia , Pérez Sevilla Gema 
Published: August 2020

AbstractBackgroundRecent years have seen a growing interest in the appearance of the eyes among the concerns expressed by patients in cosmetic clinics. This has led to an increase in the frequency of diagnosis of tear trough deformity, and, as a result, the number of treatments performed by specialized professionals has also risen. Hyaluronic acid filler injection is a rapid, nonsurgical technique that gives good long‐lasting, but not permanent, results. However, to achieve optimal results, the attending physician must have good anatomical knowledge of the area and involvement of the structures in the tear trough, carry out proper clinical assessment of the patient, and use an appropriate injection technique with the right product.AimsTo support good practice among the professionals who carry out these procedures, this interdisciplinary consensus document describes the relevant issues and recommendations, in order to improve safety standards and to help successfully resolve this aesthetic problem.