bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating metabolic and evolutionary engineering for enhanced propionic acid production under cross stress: a multi-omics perspective
Authors: Liu Tingting , Zhao Qianru , Li Yang , Zhu Liying , Jiang Ling , Huang He 
Published: December 2019

Abstract
Background: Propionic acid (PA), a potential building block for C3-based bulk chemicals, is used as a food preservative and antifungal agent because of the antimicrobial properties of its calcium-, potassium-, and sodium salts, as well as in the manufacture of pharmaceuticals, perfumes, pesticides and fungicides. However, industrial development of PA is seriously inhibited by oxygen stress, acid stress and glucose-induced osmotic stress concentration on account of the characteristic of Propionibacterium acidipropionici. To alleviate inhibition and increase PA production, enhancement P. acidipropionici tolerance to cross stress may be an effective strategy. Results: In this study, we first performed a combination of metabolic engineering (deletion of ldh and poxB and overexpression of mmc) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in P. acidipropionici. The results indicated the mutants received superior physiological activity, especially the mutant III exhibited steady 1.5-3.5 folds higher growth property and further 37.1% PA titer and 37.8% PA productivity increase than the wildtype. Moreover, omics analysis revealed the determinants such as Dps , GroES , dnaK , ADI and GAD referred to the acid adaptation of microbes were positively mobilized. ABC-type glycine betaine referred to the adaptability to osmotic stress was detected to be 2-4 folds up-regulated. More than 2-fold down-regulation of NADH oxidase and almost 3-fold up-regulation of SOD and POD were observed in three mutants. Moreover, an approximately 2.5-fold upregulation of mmc was also found.Conclusion: The multi-omics analysis revealed the multidirectional variation tendency of P. acidipropionici under cross stress and provided in-depth insights into the mechanism of tolerance and high production of PA, which layed the foundation for construction of microbial cell factories.
Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells
Authors: Gil-Kulik Paulina , Dudzińska Ewa , Radzikowska-Büchner Elżbieta , Wawer Joanna , Jojczuk Mariusz , Nogalski Adam , Wawer Genowefa Anna , Feldo Marcin , Kocki Wojciech , Cioch Maria , Bogucka-Kocka Anna , Rahnama Mansur , Kocki Janusz 
Published: December 2019

Abstract
Acute myeloid leukemia (AML) is a heterogenic lethal disorder characterized by the accumulation of abnormal myeloid progenitor cells in the bone marrow, which results in hematopoietic failure. Despite various efforts in detection and treatment, many patients with AML die of this cancer. That is why it is important to develop novel therapeutic options, employing strategic target genes involved in apoptosis and tumor progression. The aim of the study was to evaluate PARP1, PARP2, PARP3, and TRPM2 gene expression at the mRNA level in the cells of the hematopoietic system of the bone marrow in patients with acute myeloid leukemia, bone marrow collected from healthy patients, peripheral blood of healthy individuals, and hematopoietic stem cells from the peripheral blood after mobilization.Results: The results found that the bone marrow cells of patients with acute myeloid leukemia (AML) show over expression of PARP1 and PARP2 genes and decreased TRPM2 gene expression. In the hematopoietic stem cells derived from the normal marrow and peripheral blood after mobilization, the opposite situation was observed, i.e. TRPM2 gene showed increased expression while PARP1 and PARP2 gene expression was reduced. We observed the positive correlations between PARP1, PARP2, PARP3, and TRPM2 genes expression in the group of mature mononuclear cells derived from the peripheral blood and in the group of bone marrow-derived cells. In AML cells significant correlations were not observed between the expression of the examined genes.Conclusions: Our research suggests that in physiological conditions in the cells of the hematopoietic system there is mutual positive regulation of PARP1, PARP2, PARP3, and TRPM2 genes expression. PARP1, PARP2, and TRPM2 genes at mRNA level deregulate in acute myeloid leukemia cells.
Roles of neutrophil/lymphocyte ratio in prognosis and in differentiation of potential beneficiaries in HER2-positive breast cancer with trastuzumab therapy
Authors: Ding Nianhua , Huang Juan , Li Ningsha , Yuan Jiaqi , Wang Shouman , Xiao Zhi 
Published: December 2019

Abstract
Purpose: The relationship of neutrophil/lymphocyte ratio (NLR) with prognosis of HER2-positive breast cancer (BC) are not well studied. We aimed to assess the prognostic role of NLR in HER2-positive BC patients treated with or without trastuzumab.Methods: The clinical data of 843 HER2-positive BC patients from July 2013 to July 2018 were collected. The difference among variables was calculated by chi-square test. The associations between clinicopathological factors, NLR and disease-free survival (DFS) were analyzed by univariate and multivariate analyses.Results : Patients were divided into three groups. In group 1 containing 255 patients without trastuzumab treatment, pretreatment NLR showed no predictive value. Patients with trastuzumab treatment were divided into two groups on equal, according to pretreatment NLR values, low NLR (group 2) and high NLR (group 3). Patients in group 2 showed significantly higher 3-year DFS rate than patients in group 1 and group 3 (95.3% vs. 91.6% vs. 90.5%, respectively, P = 0.011); patients in the group 1 and group 3 had similar 3-year DFS outcome. Multivariate analysis showed high pretreatment NLR was significantly associated with shorter DFS (HR = 2.917, 95% CI=1.055-8.062, P = 0.039) in HER2-positive BC patients treated with trastuzumab. Conclusion : Among HER2-positive trastuzumab-treated BC patients, low pretreatment NLR value was associated with better DFS, and it might help to differentiate potential beneficiaries of trastuzumab treatment.
HAYAT SİGORTALARINDA DOKTOR İNCELEMESİ
Authors: BAHAR SAYIN Hediye 
Published: December 2019

Can sigortalarında, sigorta sözleşmesine konu rizikonun belirlenmesi açısından sigortalanacak kişinin sağlık durumuna ilişkin bilgilerin doğru şekilde tespit edilmesi önem taşır. Uygulamada söz konusu bilgiler soru formlarının yanıtlanması suretiyle elde edilir. Fakat sağlık alanındaki bilgilere hâkimiyet uzmanlık gerektirdiğinden, sigorta ettiren tarafından söz konusu bilgilerin sigortacıya doğru ve eksiksiz şekilde yansıtılması her zaman mümkün olmayabilir. Uygulamada, sigorta ettirenin soru formuna verdiği cevaplarda dikkat çekici bir durumun varlığı veya sigorta meblağının yüksek olması halinde sigortalanacak kişinin doktor incelemesinden geçirilmesinin taraflarca kararlaştırıldığı görülmektedir. Doktor incelemesine ilişkin yegâne hüküm TTK m.1492’dir. “Doktor incelemesi” kenar başlığı altında yer alan düzenlemeye göre “Sigorta ettiren ile sigortacı arasında, sigorta edilecek kişinin doktor incelemesinden geçmesi kararlaştırılmış olsa bile, sigortacı sigortalanacak kişiyi bu incelemeyi yaptırmaya zorlayamaz”. Hükmün gerekçesinde, düzenlemenin kişilik haklarını koruma altına aldığı vurgulanmaktadır. Bu çalışmada, kişilik haklarının korunması bağlamında doktor incelemesine dair ilgili hükmün amaç ve kapsamının belirlenmesi, doktor incelemesine ilişkin düzenlemenin taraf yükümlülükleri açısından değerlendirilmesi ve sigortalanacak kişinin doktor incelemesini kabul etmemesinin hukuki sonuçlarının incelemesi hedeflenmiştir
Zastosowanie różnych znaczników PET-CT w leczeniu i diagnostyce wznowy raka prostaty w oparciu o doniesienia zajazdowe z konferencji onkologicznej ASTRO 59 i przegląd literatury.
Authors: Burchardt Wojciech , Burchardt Ewa 
Published: December 2019

Rak prostaty jest drugim co do częstości nowotworem u mężczyzn w Polsce i na świecie. Wskaźniki zachorowalności ciągle rosną, dlatego dane szacunkowe mówią, że w najbliższych latach będzie to najczęściej występujący nowotwór u mężczyzn w Polsce i prawdopodobnie wyprzedzi obecnie najczęściej występującego raka płuca. Wznowa raka prostaty po leczeniu radykalnym może nawet dotyczyć ponad 50% u chorych w grupie wysokiego ryzyka i najczęściej manifestuje w postaci wzrostu stężenia antygenu sterczowego PSA (z ang. Prostate Specific Antigen). Badanie PET-CT z zastosowaniem najpopularniejszego znacznika fluror-18-dezoksy-glukozy (18F-FDG) ma słabą czułość w badaniu raka gruczołowego prostaty, dlatego jest rzadko stosowany w tym rozpoznaniu. Obecnie najczęściej stosowanym znacznikiem w diagnostyce rak prostaty w Polsce jest 11C – cholina, ale jej czułość przy stężeniu PSA poniżej 1 ng/ml nie jest dostatecznie wysoka. Pacjenci coraz częściej są poddawani badaniu PET- CT ze znakowanym galem 68 (68Ga) inhibitorem sterczowego antygenu błonowego (PSMA z ang. prostate specific membrane antygen) lub z 18-F- fluorocyclobutane-1-carboxilic acid fluciclovine (FACBC z ang. fluxiclovine), które dają możliwość zlokalizowania wznowy raka prostaty w bardzo wczesnym stadium, gdy wartość PSA jest bardzo niska (poniżej 1 ng/ml). Ponadto czułość i specyficzność badania PET-CT z zastosowaniem znacznika 68Ga-PSMA jest bardzo wysoka mimo prowadzonego leczenia antyandrogenowego, jest jednym z najbardziej obiecujących znaczników w badaniu PET.
Management of water distribution systems in PDA conditions using isolation valves: case studies of real networks
Authors: Fiorini Morosini A. , Caruso O. , Veltri P. 
Published: December 2019

Abstract
The current paper reports on a case study investigating water distribution system management in emergency conditions when it is necessary to seal off a zone with isolation valves to allow repair. In these conditions, the pressure-driven analysis (PDA) is considered to be the most efficient approach for the analysis of a water distribution network (WDN), as it takes into account whether the head in a node is adequate to ensure service. The topics of this paper are innovative because, until now, previous approaches were based on the analysis of the network behaviour in normal conditions. In emergency conditions, it is possible to measure the reliable functioning of the system by defining an objective function (OF) that helps to choose the optimal number of additional valves in order to obtain adequate system control. The OF takes into account the new network topology by excluding the zone where the broken pipe is located. The results show that the solution did not improve significantly when the number of valves reached a threshold. The procedure applied to other real case studies seems to confirm the efficiency of the methodology even if further examination of other cases in different conditions is necessary.
Association of CA 15-3 and CEA with Liver Metastases in Patients with Breast Cancer
Authors: Yazdani Akram , Akbari Hossein 
Published: December 2019

Objective:
The liver is the second most common site of distant metastasis from breast
cancer that is usually associated with poor prognosis and low quality of life in breast cancer patients.
Therefore, the primary diagnosis of liver metastatic lesions in breast cancer patients is very
important. In this study, the ability of biochemical markers CA153, CEA, and ALP to be used for
prognostic liver metastasis in women with breast cancer was investigated.

Methods:
306 women with breast cancer recorded between 2008 and 2012 were included. Serum
concentrations of alkaline phosphatase (ALP), carcinogenicity antigen (CEA), cancer antigen
(CA-153), age, menopausal status, histologic type, tumor size and number of cancerous axillary
lymph nodes in two groups of breast cancer women with liver metastases and without it were
studied. To identify independent liver metastasis prognostic factors, logistic regression method
was applied.

Results:
The independent prognostic factors of liver metastases in women with breast cancer are
ALP, CEA, age, menopausal status, number of cancerous axillary lymph nodes and tumor size.
Sensitivity and specificity analysis showed that CEA with a cutoff value of 1.1 was the most accurate
predictive factor.

Conclusion:
The increase in the levels of CEA and ALP can be diagnostic markers for liver metastases
from breast cancer.
Drug Delivery Approaches for Doxorubicin in the Management of Cancers
Authors: Vyas Manish , Simbo Daniel A. , Mursalin Mohd. , Mishra Vijay , Bashary Roqia , Khatik Gopal L. 
Published: December 2019

Aim:
We aimed to review the drug delivery approaches including a novel drug delivery
system of doxorubicin as an important anticancer drug.

Background:
Doxorubicin (DOX) is widely used against breast, uterine, ovarian, lung and cervical
cancer. It is listed among the essential medicines by WHO and is thus a very important drug
that can be used to fight against cancer. Despite its effectiveness, the use of the drug is limited due
to its dose-dependent toxicity. Several studies based on the DOX have suggested the need for
novel drug delivery formulations in the treatment of malignant and cancerous diseases due to its
cytotoxic nature.

Objectives:
This review focuses on the different formulations of DOX which is a useful drug in the
management of cancers, but associated with toxicity thus these approaches found applicability in
the reduction of its toxicity.

Methods:
We searched the scientific database using cancer, DOX, and different formulations as
the keywords. Here in only peer-reviewed research articles collected which were useful to our
current work.

Results:
This study is based on an examination of the recent advancements of its novel drug delivery
formulations. DOX hydrochloride is the first liposomal anticancer drug, administered via
the intravenous route, and also clinically approved for the treatment of lymphomas, leukemias,
and solid tumors. DOX is prepared into a liposomal formulation that contains polyethylene glycol
(PEG) layer around DOX containing liposome made by pegylation process. DOX also formulated
in nano-formulations which is also discussed herein led to reduced toxicity and increased efficacy.

Conclusion:
In the review, we described the significance of DOX in the form of different delivery
approaches in the management of cancers with a reduction in the associated toxicity.
Modeling Physico-Chemical Properties of Quinolone Derivatives Using GA-MLR as a Computational Study
Authors: Shirmohammadi Meysam , Mohammadinasab Esmat , Bayat Zakiyeh 
Published: December 2019

Background:
In this study, we used a hierarchical approach to develop quantitative
structure-activity relationship (QSAR) models for modeling physico-chemical properties of quinolone
derivatives.

Objective:
The relationship between some of the molecular descriptors with physic-chemical
properties such as refractive index (n), polarizability (α) and HOMO-LUMO energy gap (ΔEH-L)
was represented.

Materials and Methods:
Quantum mechanical calculations using abinitio method at the #HF/6-
31++G** level were carried out to obtain the optimized geometry and then, the comprehensive set
of molecular descriptors was computed by using the Dragon software. Genetic algorithm using
multiple linear regression (GA-MLR) with backward method by SPSS software were utilized to
construct QSAR models.

Results:
The analytical powers of the established theoretical models were discussed using leaveone-
out (LOO) cross-validation technique. A multi-parametric equation containing maximum
three descriptors with suitable statistical qualities was obtained for predicting the studied properties.

Conclusion:
The QSPR analysis for the prediction of the refractive index, the polarizability and
the HOMO-LUMO energy gap of 40 quinolone derivatives using GA-MLR method was performed.
The achieved results showed that the best model for predicting the refractive index, the
polarizability and the HOMO-LUMO energy gap contains maximum three descriptors. MLR
analysis, using genetic algorithms as suitable descriptors selection method showed that the three
selected descriptors play a vital role in the prediction of physicochemical properties of quinolone
derivatives. It can be noted that the best descriptors in the final obtained models can be used to design
and screen new drugs.
Community Origin and Previous Use of Antimicrobials Increase the Risk of Nosocomial Multidrug-resistant Bacteria Colonisation in the Intensive Care Unit in a Brazilian Hospital
Authors: Vandresen Debora , Werlang Maria Helena B. , Silva Mirian Carla B. , Link Juliana S. , N. Fortes Paulo Cezar 
Published: December 2019

Background:
Hospitalised patients are often surrounded by microorganisms, and antibiotic-resistant pathogens are a major and growing threat to public health.

Objective:
This study aimed to investigate the epidemiology and the risk factors for colonisation by multidrug-resistant organisms (MDROs) in a Brazilian hospital.

Methods:
Patients in the Intensive Care Unit (ICU) who underwent nasal and rectal swab cultures for the surveillance of colonisation by MDROs were evaluated in a retrospective study. MDROs were determined by routine microbiological cultures.

Results:
Of the 785 patients included in this study, 86 presented positive results for MDRO colonisation. Overall, the most frequently isolated organism was Klebsiella pneumoniae (41.9%), followed by Escherichia coli (33.7%). The main type of resistance was the production of extended-spectrum beta-lactamases (ESBL). The prevalence of MDRO infections was significantly associated with the patient's origin (community or hospital-acquired). Having been submitted to previous antimicrobial drug therapy was significantly associated with MDRO infection (relative risk [RR]: 4.02 [2.60 - 6.23]).

Conclusion:
MDRO ICU colonisation was variable, with similar frequencies as other centres, and important factors, including previous hospital stay and antibiotic use, were closely related to MDRO colonisation. Therefore, control interventions should reduce their rates, especially considering the particularities of each geographic centre.
Mathura (MBI) - A Novel Imputation Measure for Imputation of Missing Values in Medical Datasets
Authors: Bai B. Mathura , Mangathayaru N. , Rani B. Padmaja , Aljawarneh Shadi 
Published: December 2019

Aims:
Propose an imputation measure for filling missing data values so as to make the
incomplete medical datasets as complete datasets. Apply this imputation measure on imputed datasets
to achieve improved classifier accuracies.

Objective:
The basic intention of the present study is to present an imputation measure to find the
proximity between medical records and an approach for imputation of missing values in medical
datasets to improve the accuracy of existing classifiers.

Methods:
The performance of proposed approach is compared to existing approaches with respect
to classifier accuracy and also by performing non-parametric test called Wilcoxon test.

Results & Conclusion:
Experiments are conducted by considering three benchmark datasets
CLEVALAND, PIMA, ECOLI and by applying proposed imputation technique with KNN, J48
and SMO classifiers and classifier accuracies are determined. The results obtained are then compared
to thirteen existing benchmark imputation techniques available in KEEL repository. Experiment
results proved the importance of the proposed imputation technique.
Concordance Between Watson for Oncology and a Multidisciplinary Clinical Decision-Making Team for Gastric Cancer and the Prognostic Implications: Retrospective Study
Authors: Tian Yulong , Liu Xiaodong , Wang Zixuan , Cao Shougen , Liu Zimin , Ji Qinglian , Li Zequn , Sun Yuqi , Zhou Xin , Wang Daosheng , Zhou Yanbing 
Published: December 2019

Background
With the increasing number of cancer treatments, the emergence of multidisciplinary teams (MDTs) provides patients with personalized treatment options. In recent years, artificial intelligence (AI) has developed rapidly in the medical field. There has been a gradual tendency to replace traditional diagnosis and treatment with AI. IBM Watson for Oncology (WFO) has been proven to be useful for decision-making in breast cancer and lung cancer, but to date, research on gastric cancer is limited.

Objective
This study compared the concordance of WFO with MDT and investigated the impact on patient prognosis.

Methods
This study retrospectively analyzed eligible patients (N=235) with gastric cancer who were evaluated by an MDT, received corresponding recommended treatment, and underwent follow-up. Thereafter, physicians inputted the information of all patients into WFO manually, and the results were compared with the treatment programs recommended by the MDT. If the MDT treatment program was classified as “recommended” or “considered” by WFO, we considered the results concordant. All patients were divided into a concordant group and a nonconcordant group according to whether the WFO and MDT treatment programs were concordant. The prognoses of the two groups were analyzed.

Results
The overall concordance of WFO and the MDT was 54.5% (128/235) in this study. The subgroup analysis found that concordance was less likely in patients with human epidermal growth factor receptor 2 (HER2)-positive tumors than in patients with HER2-negative tumors (P=.02). Age, Eastern Cooperative Oncology Group performance status, differentiation type, and clinical stage were not found to affect concordance. Among all patients, the survival time was significantly better in concordant patients than in nonconcordant patients (P<.001). Multivariate analysis revealed that concordance was an independent prognostic factor of overall survival in patients with gastric cancer (hazard ratio 0.312 [95% CI 0.187-0.521]).

Conclusions
The treatment recommendations made by WFO and the MDT were mostly concordant in gastric cancer patients. If the WFO options are updated to include local treatment programs, the concordance will greatly improve. The HER2 status of patients with gastric cancer had a strong effect on the likelihood of concordance. Generally, survival was better in concordant patients than in nonconcordant patients.
Evaluating the Effect of Daily Diary Instructional Phrases on Respondents’ Recall Time Frames: Survey Experiment
Authors: Stone Arthur A , Wen Cheng K Fred , Schneider Stefan , Junghaenel Doerte U 
Published: December 2019

BackgroundDaily diaries are extensively used for examining participants’ daily experience in behavioral and medical science. However, little attention is paid to whether participants recall their experiences within the time frames prescribed by the task.ObjectiveThis study aimed to describe survey respondents’ self-reported recall time frames and to evaluate the impact of different daily diary items on respondents’ reported affective states.MethodsIn this study, 577 participants completed a mood survey with one of the following 4 time frame instructions: (1) today, (2) since waking up today, (3) during the last 24 hours, or (4) in the last day. They were also asked to indicate the periods they considered when answering these items and to recall the instructional phrases associated with the items.ResultsAlmost all participants in the today (141/146, 96.6%) and since waking up today (136/145, 93.8%) conditions reported using periods consistent with our expectations, whereas a lower proportion was observed in the during the last 24 hours (100/145, 69.0%) condition. A diverse range of responses was observed in the in the last day condition. Furthermore, the instructions influenced the levels of some self-reported affects, although exploratory analyses were not able to identify the mechanism underlying this finding.ConclusionsOverall, these results indicate that today and since waking up today are the most effective instructional phrases for inquiring about daily experience and that investigators should use caution when using the other 2 instructional phrases.