bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative proteomic analysis revealed maize (Zea mays L.) response to Setosphaeria turcica early infection
Authors: Liu Yuwei , Gong Xiaodong , Zhou Qihui , Liu Yajie , Liu Zhenpan , Han Jianmin , Dong Jingao , Gu Shouqin 
Published: July 2020

Abstract
Background Globally, maize (Zea mays L.) is an important crop. Maize production is severely affected by northern corn leaf blight (NCLB), a destructive foliar disease caused by Setosphaeria turcica. Interaction between maize and S. turcica has not been characterised at protein level. Results We used tandem mass tag labelling and liquid chromatography–tandem mass spectrometry to investigate the proteomes of maize leaves at 24 and 72 h post-inoculation (hpi) with S. turcica. In total, 4,740 proteins were quantified which encoded by 4,711 genes. Clustering analyses provided insight into the dynamic reprogramming of leaves proteomes by revealing the functions of different proteins during S. turcica infection. Screening and classification of differentially expressed proteins (DEPs) revealed that numerous defence-related proteins, including defence marker proteins, proteins related to the phenylpropanoid lignin biosynthesis, benzoxazinone biosynthesis and jasmonic acid signal pathway, participated in the defence responses of maize to S. turcica infection. Furthermore, the early induction of GST family proteins could contribute to the resistance of S. turcica. In addition, the protein–protein interaction network of DEPs suggested that some defence-related proteins, e.g. ZmGEB1, play key roles in defence responses against S. turcica infection. Conclusion This study provides insight into the complex defence responses triggered by S. turcica at the protein level, as well as a new basis for studying the interaction process between maize and S. turcica at the earlier stage of infection.
Clinical Characteristics and outcomes in HBV carriers with COVID-19 in WuHan, China: a retrospective cohort study
Authors: Lu Jingjing , Hu Mu , Zhou Xia , Zhu Hui , Wang Feilong , Huang Jianhao , Guo Zhongliang , Li Qiang , Yin Qi , Yang Zhifeng 
Published: July 2020

Abstract
Background: Coronavirus 2019 (COVID-19) is a novel infectious disease that was first reported in Wuhan, China, but has spread to all parts of the world. At the same time, because China has millions of HBV carriers, HBV infection has become a major public health problem in China. In this study, we aim to describe the clinical features of HBV carriers (AsC) infected with COVID-19 and to assess the factors that may affect the outcome during disease progression.Methods: This retrospective cohort study included 72 patients diagnosed with COVID-19 in Wuhan Jinyintan Hospital. These patients were also diagnosed as HBV carriers. The epidemiological characteristics, demographic features, clinical manifestations, laboratory test, treatment, management and final outcome were collected and analyzed.Results: The median age of 72 patients is 58.5 years old, of which 55.56% (n=40) are male. 20 (30.56%) patients were severe cases and 50 (69.44%) were non-severe cases. Fever is the most common symptom, followed by cough, chest tightness and sputum. Laboratory test results including hematologic, biochemical, infection and coagulation parameters and several indicators, such as Aspartate Aminotransferase (AST), Total Bilirubin (TBil), Direct Bilirubin (DBil), Indirect Bilirubin (IBil), γ-glutamyl Transferase (GGT) showed difference between their admission and discharge. The level of Prealbumin (PA) and Serum Amyloid A (SAA) in the study showed a significant trend from high to low, which has statistical significance.Conclusions: The clinical features of HBV carriers with COVID-19 have obvious systemic symptoms, such as fever, cough, and chest tightness. Compared with liver function data on admission and discharge, SARS-CoV-2 does not directly activate the Hepatitis B virus, and the risk of liver cell damage of HBV carriers with COVID-19 does not increase. Both PA and SAA are sensitive indicators and can be used to evaluate the prognosis and outcome of these patients.
Determine the IgG and IgM antibodies created against T. gondii infections using the ELISA method in diabetic pregnant women in compared with non -diabetic pregnant women in Sanandaj, Kurdistan, west of Iran
Authors: Bahmani nasrin , Shakiba Nadia , Farhadifar Fariba , Zareei Mojde 
Published: July 2020

Abstract
Background: Toxoplasma gondii is an opportunistic parasitic protozoan, which is a causative agent of serious complications such as abortion or fetal still birth in pregnant women. The purpose of this study was evaluating the seroprevalence of toxoplasmosis and risk factors in diabetic pregnant women (case) in comparison with non-diabetic pregnant women (control) from Sanandaj, Kurdistan, west of Iran. Methods: In this case-control study Toxoplasma gondii antibodies were tested in 91 cases as well as 90 controls from referred the Toohid hospital diabetes center using ELISA (enzyme-linked immunosorbent assay) method during June 2018 to October 2019 in Sanandaj, Kurdistan, west of Iran. The collected data were analyzed by SPSS 19 and using Chi-Square and Fisher tests.Results: The seropositivity for anti-Toxoplasma IgG in case group was 33% and 15.6% in the control group (p = 0.006) showing a statistically significant difference between groups, while for anti-Toxoplasma IgM (p = 0.31) showing no statistically significant differences between two groups. A significant relationship was observed between age (p = 0.024) and abortion history (p = 0.05) with anti-Toxoplasma IgG antibodies in cases and controls.Conclusion: The results of current study indicated statistically significant relationship between diabetes and toxoplasmosis. The abortion history and increase of age was found to be a major T. gondii infection risk factor.
The influenza vaccine for nursing and care professionals at emergency services in Flanders
Authors: Samyn Matthijs , Hal Guido Van , Vandevijvere Herman , Damme Pierre Van 
Published: July 2020

Abstract
Background: The Belgian Superior Health Council recommends seasonal influenza vaccination for people working in the health care sector in order to reduce the risk of the influenza virus being transmitted to people at risk. The aim of this study is to provide more insight into the current vaccination rate in nurses and health care professionals in emergency services in Flanders. In addition, the influence of influenza vaccination on absenteeism will be investigated, as well as the motivation to get vaccinated or not.Methods: A quantitative multicentre, cross-sectional study was carried out in which nurses and health care professionals in emergency services in Flanders were questioned by means of an online questionnaire.Results: The survey of nurses and health care professionals in Flanders from 2018 shows that 54% (294/548) gets vaccinated annually. Respondents who have been vaccinated report a lower absenteeism due to flu compared to health workers who do not get vaccinated. There is a lack of general knowledge about flu and vaccination. 34% of nurses and health care professionals in emergencies do not know the correct definition of flu. The main reasons for being vaccinated are: to protect oneself (27%), family members (21.5%) and patients (16%). Reasons for not getting vaccinated are: never having had the flu before (30%), believing you get the flu because of the vaccination (12%), no belief or trust in the vaccine (19%).Conclusions: This study shows that influenza vaccination campaigns should put the emphasis on knowledge. Nursing and care professionals should be convinced of the effective action of the vaccine. This can increase the vaccination rate for seasonal influenza and as a consequence reduce absenteeism.
Usefulness of K-line in predicting prognosis of laminoplasty for cervical spondylotic myelopathy
Authors: Ikeda Terumasa , Miyamto Hiroshi , Akagi Masao 
Published: July 2020

Abstract
Background: K-line is widely recognized as a useful index to evaluate alignment and size of the cervical ossification of the posterior longitudinal ligament (OPLL) in one parameter. The purpose of this study was to investigate that K-line could be a tool to predict the prognosis of LP for cervical spondylotic myelopathy (CSM) as well. Methods: Sixty-eight patients who underwent LP were enrolled. C2-7 angle, local kyphosis angle, and K-line which is the straight line connecting the midpoints of the spinal canal at C2 and C7 was evaluated on T2- weighted sagittal magnetic resonance imaging (MRI). The JOA score and the recovery rate of the JOA score were evaluated at pre-operation and at follow-up. C2/C7 angle, local kyphosis angle, the JOA score, and the recovery rate were compared between K-line (-) and K-line (+) groups. Results: The recovery rate of K-line (+) group (50.6%) was significantly better than that of K-line (-) (19.4%). In K-line (-), the disc type in which the protruded disc was absorbed during the follow-up showed statistically better recovery rate (27.6%) at follow-up compared to other K-line (-) in which anterior cord compression due to the osteophyte or the kyphotic beak was not absorbed (osseous type, 5.0%).Conclusion: The present study has indicated that K-line can be a factor to predict the clinical outcome of LP for CSM. In K-line (-), the disc type showed somewhat better outcomes compared to the osseous type. However, the results were not sufficient.
Gunshot Injuries: Patterns, Presentations, and Outcomes of Civilian Hospital Experiences in a Developing Country Setting
Authors: GISMALLA MOHAMED Daffalla Awadalla , Bakhiet Mohammed , Koko Mohammedbabalrahma , Kabbashi Abdelrahim 
Published: July 2020

Abstract
BackgroundGunshot injuries are considered a burden as well as one of the intricate emergencies in civilian medical practice. In this study we aim to determine the pattern of presentation and management outcome in a general hospital in a sub-Saharan country.MethodsThis is a retrospective, hospital-based study conducted between January 2015- December 2019 in a general teaching hospital, to review the clinical presentation and management outcome of gunshot injuries. All patients’ records were reviewed during the study period.ResultsOne hundred fifty-seven patients were included in the study, with male predominant 83%. 40% are between 19-30 years of age, with patients under 18 years of age comprising 17% of the population. The anatomical sites most commonly affected by gunshot were the lower limb (41.4%) and upper limb (22.9%), and the least impacted site is the perineum (1.9%). The most common diagnoses among the patients in this study were limb fractures (49.7%) and soft tissue injuries (28.7%). Specific treatment in this study is wound debridement, which was done for 91 (58.0 %) patients. Bone fixation, laparotomy and chest tube insertion were done for the rest of the patients. More than 120 (75%) of patients were treated and discharge in a good condition and 27 (17.2%). The mortality rate in this study group was 15.3 %.Conclusion Most of the patient in this study were young males. Upper and lower limb fractures are the most common presentations. Wound debridement, bone fixation and laparotomy are the most common treatments with significant success rates, despite prolonged hospital stay.
In Silico Approach of Potential Phytochemical Inhibitor from Moringa oleifera, Cocos nucifera, Allium cepa, Psidium guajava, and Eucalyptus globulus for the treatment of COVID-19 by Molecular Docking
Authors: Fitriani Ika Nur , Utami Wiji , Zikri Adi Tiara , Santoso Pugoh 
Published: July 2020

Abstract
Background

Coronavirus disease 2019 (COVID-19) is caused by infection with severe acute respiratory syndrome coronavirus 2. COVID-19 has devastating effects on people in all countries and getting worse. We aim to investigate an in-silico docking analysis of phytochemical compounds from medicinal plants that used to combat inhibition of the COVID-19 pathway. There are several phytochemicals in medicinal plants, however, the mechanism of bioactive compounds remains unclear. These results are obtained from in silico research provide further information to support the inhibition of several phytochemicals.
Methods

Molecular docking used to determine the best potential COVID-19 M pro inhibitor from several bioactive compounds in Moringa oleifera, Allium cepa, Cocos nucifera, Psidium guajava, and Eucalyptus globulus. Molecular docking was conducted and scored by comparison with standard drugs remdesivir. ADME properties of selected ligands were evaluated using the Lipinski Rule. The interaction mechanism of the most recommended compound predicted using the STITCH database.
Results

There was no recommended compound in Moringa oleifera as a potential inhibitor for COVID-19. Oleanolic acid in Allium cepa, α-tocotrienol in Cocos nucifera, asiatic acid in Psidium guajava and culinoside in Eucalyptus globulus were the most recommended compound in each medicinal plant. Oleanolic acid was reported to exhibit anti-COVID-19 activity with binding energy was − 9.20 kcal/mol. This score was better than remdesivir as standard drug. Oleanolic acid interacted through the hydrogen bond with HIS41, THR25, CYS44, GLU166. Oleanolic acid binding with CASP-3, CASP-9, and XIAP signaling pathway.
Conclusions

Oleanolic acid in Allium cepa found as a potential inhibitor of COVID-19 M-pro that should be examined in future studies. These results suggest that oleanolic acid may be useful in COVID-19 treatment.
Plastic in global rivers: are floods making it worse?
Authors: Roebroek Caspar T.J. , Harrigan Shaun , Emmerik Tim van , Baugh Calum , Eilander Dirk , Prudhomme C. , Pappenberger Florian 
Published: July 2020

Abstract
Riverine plastic pollution is of global concern due to its negative impact on ecosystem health and human livelihood. Recent studies show a strong link between river discharge and plastic transport, but the role of floods is still unresolved. We combined high resolution mismanaged plastic waste data and river flood extents with increasing return periods to estimate flood-driven plastic mobilisation, aggregated at administration unit, country and global scale. We show that 10-year return period floods already tenfold the global plastic mobilisation potential compared to non-flood conditions. In the worst affected regions, plastic mobilisation increases up to five orders of magnitude. Our results suggest a high inter-annual variability in plastic mobilisation, previously ignored by global plastic transport models. Flood defences reduce plastic mobilisation substantially, but regions vulnerable to flooding often coincide with high plastic mobilisation potential during floods. Consequentially, clean-up and mitigation measures and flood risk management are inherently interdependent and need to be managed holistically.
Percutaneous fully endoscopic surgical management of the ruptured epidural catheter: Rescue of the young anesthesiologist from his dilemma
Authors: Kong Weijun , Cao Guangru , Liu Dexing , Du Qian , Wei Yiyong , Liao Wenbo 
Published: July 2020

Abstract
Background

Epidural nerve block and analgesia are basic operation techniques for anesthesia. Epidural catheter rupture and partial retention are adverse events and rare complications of epidural catheterization technology. The probability of occurrence when applied by young doctors is high. Removal of the residual catheter by conventional surgery causes more trauma and bleeding, slows recovery, and causes medical disputes. These events also challenge the growth of young doctors. Some hospitals have almost eliminated this operation technique. All surgical patients undergo intubation and general anesthesia, which greatly wastes medical resources and increases patient costs.
Objective

To analyze the efficacy and safety of removing the residual dural catheter by a percutaneous endoscopic technique and discuss the clinical technique and precautions.
Methods

This was a retrospective analysis of 7 patients with ruptured epidural catheters treated in our department from October 2015 to October 2019 using the percutaneous spinal endoscopic technique to remove the remaining epidural catheter. The operation time, intraoperative bleeding volume, surgical complications, and neurological symptoms before and after surgery were recorded. The Self-Rating Anxiety Scale (SAS) was used to assess the anxiety level of the anesthesiologist and the patient before and after the catheter removal operation, and the postoperative low back pain VAS score was recorded.
Results

The remaining epidural catheter was successfully removed from all 7 patients. The operation time was 54.14 ± 14.45 (32–78) minutes, and the intraoperative blood loss was 9.134 ± 3.078 (5–15) ml. There were no cases of dural damage, cerebrospinal fluid leakage, sensorimotor dysfunction of the lower limbs, or bowel dysfunction. The anxiety symptoms of the patient and the anesthesiologist disappeared after removal of the residual epidural catheter. The patients' postoperative back pain VAS score was 0 to 2 points.
Conclusion

Percutaneous spinal endoscopy is a safe and effective minimally invasive technique for removing residual epidural catheters. It causes less trauma and less bleeding, allows a faster recovery, and has a low cost. It does not affect the recovery of patients from other surgical operations and reduces both medical risks and medical costs. At the same time, it avoids or reduces the occurrence of medical disputes and eliminates the pressure on young anesthesiologists regarding similar adverse events.
Ameliorative Effects of Nano-Selenium Against Fluoride Stress Induced Hepatocytes Autophagy and Apoptosis in Mice
Authors: Wang Yajing , Liu Bingxian , Han Qingyue , Mehmood Khalid , Nabi Fazul , Chang Yung-Fu , Tang Zhaoxin , Li Ying , Zhang Hui 
Published: July 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
DNP-Supported Solid-State NMR Studies of 13C,15N,29Si-Enriched Biosilica of Cyclotella Cryptica and Thalassiosira Pseudonana
Authors: Ehren Helena , Kolbe Felicitas , Paioni Alesandra Lucini , Brunner Eike , Baldus Marc 
Published: July 2020

Abstract
Solid-state NMR spectroscopy represents a powerful method for the investigation of diatom biosilica but detailed studies regarding its chemical composition and structural organization can be prohibited by insufficient spectroscopic sensitivity. Here, we used two-dimensional (2D) Dynamic Nuclear Polarization (DNP)-supported solid-state NMR experiments to obtain information about the molecular composition and supramolecular organization of proteins and carbohydrates in13C,15N,29Si-labeled biosilica of C. cryptica. As a reference, we conducted DNP experiments on isotope-labeled biosilica of T. pseudonana. DNP-enhancement factors for different NMR signals, and thus, for different organic compounds, provide information about the supramolecular architecture of the biosilica. In addition, DNP-supported heteronuclear nitrogen-carbon correlation experiments allowed us to prove the presence of different LCPAs (long chain polyamines) and revealed the occurrence of amine-nitrogen moieties exhibiting a correlation with carbonyl carbons that may indicate cross-linking of LCPAs to proteins as previously seen in studies on proteins extracted from other diatoms.
Psychometric Validation Of The French Self And Proxy Versions Of The PedsqlTM 4.0 Generic Health-Related Quality Of Life Questionnaire For 8 To 12 Year-Old Children.
Authors: Amedro Pascal , Huguet Helena , Macioce Valerie , Dorka Raphael , Auer Annie , Guillaumont Sophie , Auquier Pascal , Abassi Hamouda , Picot Marie-Christine 
Published: July 2020

Abstract
Background: The Pediatric Quality of Life Inventory Version 4.0 (PedsQLTM4.0) is a generic health-related quality of life (HR-QoL) questionnaire, widely used in pediatric clinical trials but not yet validated in France. We performed the psychometric validation of the self and proxy PedsQLTM4.0 generic questionnaires for French children aged 8 to 12 years old. Methods: The multicenter prospective study included 123 children and their parents with congenital heart disease (CHD) and 97 controls. Results: Test–retest reliability intra-class correlation coefficients were mainly in good range (0.49-0.66) for both self and proxy reports. Face validity was very good among parents (0.85) and children (0.75). Content validity was good (0.70), despite misinterpretation of some items. In construct validity, each subscale had good internal consistency reliability (Cronbach's α >0.72 in self-reports, >0.69 in proxy-reports). In the confirmatory factor analysis, the goodness-of-fit statistics rejected the original structure with 4 factors. The exploratory factor analysis revealed an alternative two-factor structure corresponding to physical and psychological dimensions. Convergent validity was supported by moderate (>0.41) to high correlations (0.57) between PedsQL and Kidscreeen questionnaires for physical, emotion and school dimensions. The ability of the PedsQL to discriminate CHD severity was better with physical, social and total scores for both self-reports and proxy-reports.Conclusions: The PedsQLTM4.0 generic self and proxy HR-QoL questionnaires found good psychometric properties, with regard to acceptability, responsiveness, validity, and reliability. This instrument appeared to be easy to use and comprehend within the target population of children aged 8 to 12 years old and their parents. Trial registration: This study was approved by the South-Mediterranean-IV Ethics Committee and registered on ClinicalTrials.gov (NCT01202916) on 16 Sepetmber 2020, https://clinicaltrials.gov/ct2/show/NCT01202916.
Is Wide-Awake Local Anesthesia No Tourniquet Technique in Distal Radius Plating Surgery a Comfortable Procedure? A Prospective Case-Control Study
Authors: Lu Chun-Kuan , Liu Wen-Chih , Lu I-Chen , Chang Chung-Chia , Shih Chia-Lung , Fu Yin-Chih , Jupiter Jesse Bernard 
Published: July 2020

Abstract
Background: Wide-awake local anesthesia no tourniquet (WALANT) is a promising technique for bony procedure in recent years. During distal radius surgery via WALANT technique, surgeons may concern about intraoperative pain. This prospective case-control study was aimed to compare the intraoperative hemodynamic changes between WALANT technique and general anesthesia (GA) in patients undergoing distal radius plating surgery.Methods: We recruited 40 adults with distal radius fracture underwent plating using the WALANT technique (group A) or general anesthesia (group B). Each group comprised 20 patients with similar demographics. Mean arterial pressure (MAP) and heart rate were recorded in both groups. Intraoperative pain intensity was measured by numeric rating scale (NRS) for pain in group A.Results: The NRS decreased significantly compared with preoperative status in group A for most of the intraoperative period. The intraoperative MAP in group A showed no significant change among each peri-operatively period. In addition, group A showed less peri-operative MAP fluctuation than group B (p< 0.05). The intraoperative changes in HR showed significant changes between group A and B during reduction, plating and skin closure. The reduction and plating quality were similar between each group.Conclusions: Patients undergoing distal radius plating surgery via WALANT technique has a lower MAP changes. Distal radius plating surgery using the WALANT technique is a well-tolerated surgical procedure and showing the similar reduction and plating quality to through GA.