bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving mucosal anesthesia for awake endotracheal intubation with a novel method: A prospective, assessor-blinded, randomized controlled trial
Authors: Han Chunji , Li Peng , Guo Zhenggang , Guo Ying , Sun Li , Chen Gang , Qiu Xiaojue , Mi Weidong , Zhang Changsheng , Berra Lorenzo 
Published: December 2020

Abstract
Background:Topical anesthesia is a crucial step in awake endotracheal intubation for providing favorable intubation conditions. The standard of care technique for awake intubation at our institution, which consists of oropharyngeal tetracaine spray, can result in inadequate mucosal anesthesia. Therefore, we sought to compare the effectiveness of dyclonine hydrochloride mucilage to the standard of care tetracaine in achieving anesthesia of the upper airways for awake endotracheal intubation.Methods:This is a randomized, assessor-blinded, prospective study. From Jun. 1st, 2019 to Aug. 1st, 2019, patients scheduled for either endoscopic submucosal dissection or peroral endoscopic myotomy were enrolled and randomly allocated into two groups after obtaining written informed consent: patients allocated to novel awake intubation care (Group N-AIC) received a single administration of oral dyclonine hydrochloride mucilage, whereas patients allocated to standard awake intubation care (Group S-AIC) received three oropharyngeal tetracaine sprays before transcricoid tetracaine injection before awake intubation. Mean arterial pressure (MAP), which was the primary outcome of this study, as well as heart rate (HR) were recorded throughout the procedure and compared between the two groups. Feeling of numbness, nausea, and intubation conditions after topical anesthesia were also assessed.Results:Sixty patients were enrolled and completed the study. Baseline MAP and HR were similar between the two groups. However, hemodynamic responses to intubation and gastrointestinal endoscopy, especially MAP, were significantly less elevated in Group N-AIC. The degree of numbness of the oropharyngeal mucosa after topical anesthesia did not differ between the two groups, neither did the feeling of nausea during laryngoscopy. The amount of pharyngeal secretions before intubation was less in Group N-AIC. Total intubation time was significantly shorter in Group N-AIC when compared to Group S-AIC (18.4 ± 2.86 vs. 22.3 ± 6.47, P < 0.05). Extubation bucking was significantly less frequent in Group N-AIC (13.3% vs. 76.7%). Patients received in Group N-AIC had a lower rate of post-extubation sore throat compared to Group S-AIC (6.7% vs. 43.3%). No adverse side effects attributable to either tetracaine or dyclonine were observed in this study.Conclusions:In awake endotracheal intubation, novel care using oral dyclonine hydrochloride mucilage can provide more favorable mucosal anesthesia and better intubation conditions compared to standard of care practice using oropharyngeal tetracaine spray.Chinese Clinical Trial Registry: ChiCTR1900023151 ( http://www.chictr.org )
An Investigation Into the Cause of Road Failure Along Sagamu-Papalanto Highway Southwestern Nigeria
Authors: UBIDO OYEM EMMANUEL , Ogbonnaya Igwe , Ukah Bernadette Uche 
Published: December 2020

Abstract
Investigation into the cause of road failure has been carried out along a 60km long Sagamu –Papalanto highway southwestern Nigeria. Geochemical, mineralogical, geotechnical and geophysical analyses were conducted to evaluate the cause of failure along the study area. The results of the laboratory tests showed that the percentage amount of fines ranges from 12-61.3%, natural moisture content from 6.8 to 19.7%, liquid limit in the range of 25.1-52.2%, linear shrinkage between 3.96 to 12.71%, plastic limit ranges from 18.2-35%, plasticity index ranges from 5.2 to 24.6%, free swell in the range from 5.17 – 43.9%, maximum dry density ranges from 1.51 -1.74g /cm3, specific gravity ranges from 2.52-2.64 and CBR between 3-12%. The Cone Penetrometer Test (CPT) shows a resistance value of 20-138 kgf/cm2. The major clay mineral that is predominant in the studied soil is kaolinite. The major oxides present are SiO2, Al2O3, Fe2O3, K2O, Na2O, MgO and CaO. The result of the 2D Electrical Resistivity Imaging revealed a low resistivity values for profile 2 and 3 ranging from 100 Ωm – 300 Ωm, between a distance of 20m – 240 m along the profile to a depth of 7.60m and a low resistivity value ranging from 50 Ωm – 111Ωm, between a distance of 80 m –120 m along the profile to a depth of 15m. It was concluded that the low CBR, low MDD and the class of subsoils namely A-26, A-7, A-2-7 (clayey soils) which were identified are responsible for the cause of failure experienced in the study area. These makes the soils unsuitable as road construction materials and hence, there is need for stabilization during the reconstruction and rehabilitation of the road.
An “expressionistic” look at serrated precancerous colorectal lesions
Authors: Marra Giancarlo 
Published: December 2020

Abstract
Background: Approximately 60% of colorectal cancer (CRC) precursor lesions are the genuinely-dysplastic conventional adenomas (cADNs). The others include hyperplastic polyps (HPs), sessile serrated lesions (SSL), and traditional serrated adenomas (TSAs), subtypes of a class of lesions collectively referred to as “serrated.” Endoscopic and histologic differentiation between cADNs and serrated lesions, and between serrated lesion subtypes can be difficult. Methods: We used in situ hybridization to verify the expression patterns in CRC precursors of 21 RNA molecules that appear to be promising differentiation markers on the basis of previous RNA sequencing studies.Results: SSLs could be clearly differentiated from cADNs by the expression patterns of 9 of the 12 RNAs tested for this purpose (VSIG1, ANXA10, ACHE, SEMG1, AQP5, LINC00520, ZIC5/2, FOXD1, NKD1). Expression patterns of all 9 in HPs were similar to those in SSLs. Nine putatively HP-specific RNAs were also investigated, but none could be confirmed as such: most (e.g., HOXD13 and HOXB13), proved instead to be markers of the normal mucosa in the distal colon and rectum, where most HPs arise. TSAs displayed mixed staining patterns reflecting the presence of serrated and dysplastic glands in the same lesion. Conclusions: Using a robust in situ hybridization protocol, we identified promising tissue-staining markers that, if validated in larger series of lesions, could facilitate more precise histologic classification of CRC precursors and, consequently, more tailored clinical follow-up of their carriers. Our findings should also fuel functional studies on the pathogenic significance of specific gene expression alterations in the initiation and evolution of CRC precursor subtypes.
Propensity score-matched analysis of systemic chemotherapy versus salvage hysterectomy for persistent cervical cancer after definitive radiotherapy/concurrent chemoradiotherapy.
Authors: Takekuma Munetaka , Takahashi Fumiaki , Mabuchi Seiji , Kudaka Wataru , Horie Koji , Ikeda Mariko , Shikama Ayumi , Mitsuhashi Akira , Nagao Shoji , Suzuki Shiro , Mizuno Mika , Nishio Shin , Tokunaga Hideki , Ota Yukinobu , Kasamatsu Takahiro , Kitagawa Ryo , Toita Takafumi , Kobayashi Hiroaki , Ishikawa Mitsuya , Yaegashi Nobuo 
Published: December 2020

Abstract
Background: The aim of the current study was to evaluate oncologic outcomes of patients who were treated with salvage hysterectomy (HT), compared to systemic chemotherapy (CT) for persistent cervical cancer after definitive radiotherapy (RT)/ concurrent chemoradiotherapy (CCRT).Methods: Patients with persistent cervical cancer treated with definitive RT/CCRT at 35 institutions from 2005–2014 were reviewed retrospectively (n = 317). Those who underwent a HT for persistent cervical cancer after definitive RT/CCRT were matched with propensity scores for patients who underwent systemic CT. Oncologic outcomes between the two groups using a propensity score matched–cohort analysis were compared.Results: A total of 142 patients with persistent cervical cancer after definitive RT/CCRT were included after matching (HT: 71, systemic CT: 71). All background factors between HT and CT groups were well balanced. Median overall survival was 3.8 and 1.5 years in the HT and CT groups, respectively (p= 0.00193, hazards ratio [HR] 0.41, 95% confidence interval [CI] 0.23–0.73), Increasing residual tumor size was significantly associated with a high incomplete resection rate (p = 0.016, Odds Ratio 1.11, 95%CI 1.02-1.22). Severe late adverse events occurred in 7 patients (9.9%) in the HT cohort.Conclusion: The current study demonstrated that, when compared to systemic CT, the adoption of salvage HT for patients with persistent cervical cancer after definitive RT/CCRT reduced mortality rate by about 60%. This indicates that salvage HT could be curative treatment for those patients. Further prospective clinical trials with regard to salvage HT after RT/CCRT are warranted.
De novo transcriptome in roots of switchgrass (Panicum virgatum L.) reveals gene expression dynamic and act network under alkaline salt stress
Authors: Zhang Pan , Duo Tianqi , Wang Fengdan , Zhang Xunzhong , Yang Zouzhuan , Hu Guofu 
Published: December 2020

Abstract
Background: Soil salinization is a major limiting factor for crop cultivation. Switchgrass is a perennial rhizomatous bunchgrass that is considered an ideal plant for marginal lands, including sites with saline soil. Here, we investigated the physiological responses and transcriptome changes in the roots of Alamo (alkali-tolerant genotype) and AM-314/MS-155 (alkali-sensitive genotype) under alkaline salt stress.Results: Alkaline salt stress significantly affected the membrane, osmotic adjustment and antioxidant systems in switchgrass roots, and the ASTTI values between Alamo and AM-314/MS-155 were divergent at different time points. A total of 108,319 unigenes were obtained after reassembly, including 73,636 unigenes in AM-314/MS-155 and 65,492 unigenes in Alamo. A total of 10,219 DEGs were identified, and the number of upregulated genes in Alamo was much greater than that in AM-314/MS-155 in both the early and late stages of alkaline salt stress. The DEGs in AM-314/MS-155 were mainly concentrated in the early stage, while Alamo showed greater advantages in the late stage. These DEGs were mainly enriched in plant-pathogen interactions, ubiquitin-mediated proteolysis and glycolysis/gluconeogenesis pathways. We characterized 1,480 TF genes into 64 TF families, and the most abundant TF family was the C2H2 family, followed by the bZIP and bHLH families. A total of 1,718 PKs were predicted, including CaMK, CDPK, MAPK and RLK. WGCNA revealed that the DEGs in the blue, brown, dark magenta and light steel blue 1 modules were associated with the physiological changes in roots of switchgrass under alkaline salt stress. The consistency between the qRT-PCR and RNA-Seq results confirmed the reliability of the RNA-seq sequencing data. A molecular regulatory network of the switchgrass response to alkaline salt stress was preliminarily constructed on the basis of transcriptional regulation and functional genes.Conclusions: The alkaline salt tolerance of switchgrass may be achieved by the regulation of ion homeostasis, transport proteins, detoxification, heat shock proteins, dehydration and sugar metabolism. These findings provide a comprehensive analysis of gene expression dynamic and act network induced by alkaline salt stress in two switchgrass genotypes and contribute to the understanding of the alkaline salt tolerance mechanism of switchgrass and the improvement of switchgrass germplasm.
Identification and Analysis of Long Non-coding RNAs and mRNAs in Chicken Macrophages Infected with Avian Infectious Bronchitis Coronavirus
Authors: Li Hao , Cui Pengfei , Fu Xue , Zhang Lan , Yan Wenjun , Zhai Yaru , Lei Changwei , Wang Hongning , yang xin 
Published: December 2020

Abstract
Background: Avian infectious bronchitis virus (IBV) is a gammacoronavirus that severely affects the poultry industry worldwide. Long non-coding RNAs (lncRNAs), a subset of non-coding RNAs with a length of more than 200 nucleotides, have been recently recognized as pivotal factors in the pathogenesis of viral infections. However, little is known about the function of lncRNAs in host cultured cells in response to IBV infection.Results: We used next-generation high throughput sequencing to reveal the expression profiles of mRNAs and lncRNAs in IBV-infected HD11 cells. Compared with the uninfected cells, we identified 153 differently expressed (DE) mRNAs (106 up-regulated mRNAs, 47 down-regulated mRNAs) and 181 DE lncRNAs (59 up-regulated lncRNAs, 122 down-regulated lncRNAs) in IBV-infected HD11 cells. Moreover, gene ontology (GO) and pathway enrichment analyses indicated that DE mRNAs and lncRNAs were mainly involved in cellular innate immunity, amino acid metabolism, and nucleic acid metabolism. In addition, 2,640 novel chicken lncRNAs were identified, and a competitive endogenous RNA (ceRNAs) network centered on gga-miR-30d and miR-146a-5p was established. Conclusions: The present study identified the expression profiles of mRNAs and lncRNAs during IBV infection and provides new insights into the pathogenesis of IBV.
Association between lipoprotein cholesterol and future cardiovascular disease and mortality in older adults: a Korean nationwide longitudinal study
Authors: Kim Seung Hee , Son Ki Young 
Published: December 2020

Abstract
Background Dyslipidemia is considered an independent health risk factor of cardiovascular disease (CVD), a leading cause of mortality in older adults. Despite its importance, there have been few reports on the association between lipoprotein cholesterol and future CVD and cardiovascular (CV) mortality among elderly Asians aged ≥65 years. This study investigated the association between lipoprotein cholesterol and future CVD and CV mortality in an elderly Korean population using a large nationwide sample.Methods From the cohort database of the Korean National Health Insurance Service, 62,604 adults aged ≥65 years (32,584 men and 30,020 women) were included. High-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels were categorized by quartiles. Cox proportional hazard models and linear regression analyses were used to assess the association between the quartiles of lipoprotein cholesterol and future CV events or mortality.Results The mean follow-up period was 3.3 years. The incidence rates of ischemic heart disease and ischemic brain disease were 0.97 and 0.61 per 1,000 person-years, respectively, and the mortality rates from these diseases were 0.22 and 0.34 per 1,000 person-years, respectively. In a completely adjusted model, high HDL-C and LDL-C levels were not associated with total CV events and CVD mortality. However, high LDL-C levels were significantly associated with a lower incidence of ischemic brain disease. Furthermore, diabetic patients with high LDL-C levels were more likely to have higher CV mortality, whereas non-smokers with high LDL-C levels were less likely to be at risk of CV events.Conclusions Neither high LDL-C nor HDL-C levels were significantly associated with future CV mortality in older adults aged ≥65 years. High LDL-C levels do not seem to be a risk factor for CVD in elderly individuals, and further studies are required.
Comparison of analgesic efficacy between rectus sheath blockade, intrathecal morphine with bupivacaine, and intravenous patient-controlled analgesia in patients undergoing robot-assisted laparoscopic prostatectomy: a prospective, observational clinical study
Authors: Shim Jung-Woo , Cho Yun Jeong , Kim Minhee , Hong Sang Hyun , Moon Hyong Woo , Hong Sung Hoo , Chae Min Suk 
Published: December 2020

Abstract
Background: We explored the analgesic outcomes on postoperative day (POD) 1 in patients undergoing robot-assisted laparoscopic prostatectomy (RALP) who received intravenous patient-controlled analgesia (IV-PCA), rectus sheath bupivacaine block (RSB), or intrathecal morphine with bupivacaine block (ITMB). Methods: This was a prospective, observational clinical trial. Patients were divided into three groups: IV-PCA (n = 30), RSB (n = 30), and ITMB (n = 30). Peak pain scores at rest and with coughing, cumulative IV-PCA drug consumption, the need for IV rescue opioids, and Quality of Recovery-15 (QoR-15) questionnaire scores collected on POD 1 were compared among the groups.Results: The preoperative and intraoperative findings were comparable among the groups; the ITMB group required the least remifentanil of all groups. During POD 1, the ITMB group reported lower levels of pain at rest and with coughing, compared with the other two groups. During POD 1, incidences of severe pain at rest (10.0% vs. 23.3% vs. 40.0%) and with coughing (16.7% vs. 36.7% vs. 66.7%) were the lowest in the ITMB group compared with the RSB and IV-PCA groups, respectively. After adjustment for age, body mass index, diabetes mellitus, hypertension, and intraoperative remifentanil infusion, severe pain at rest was 0.167-fold less common in the ITMB group than in the IV-PCA group, while pain with coughing was 0.1-fold lower in the ITMB group and 0.306-fold lower in the RSB group, compared with the IV-PCA group. The ITMB group required lower cumulative IV-PCA drug infusions and less IV rescue opioids, while exhibiting a better QoR-15 global score, compared with the other two groups. Complications (nausea and pruritus) were significantly more common in the ITMB group than in the other two groups; however, we noted no ITMB- or RSB-related anesthetic complications (respiratory depression, post-dural headache, nerve injury, or puncture site hematoma or infection), and all patients were assessed as Clavien-Dindo grade I or II during the hospital stay.Conclusion: Although ITMB induced complications of nausea and pruritus, this analgesic technique provided appropriate pain relief that enhanced patient perception related to early postoperative recovery.Trial registration: Clinical Research Information Service, Republic of Korea, (approval number: KCT0005040) on May 20, 2020https://cris.nih.go.kr/cris/search/search_result_st01_kren.jsp?seq=15943&sLeft=2&ltype=my&rtype=my
Behavior and origin of hydrogen defects in natural orthopyroxene during high-temperature processes
Authors: Yang Yan , Ingrin Jannick , Liu Wendi , Huang Weihua , Gu Xiaoyan , Xia Qunke 
Published: December 2020

Abstract
Spectral features of hydrogen defects in natural mantle minerals derive from physico-chemical conditions of the lithosphere. Although hydrogen defects in synthetic orthopyroxene have been well investigated, their complex spectral features in natural orthopyroxenes are still difficult to decipher. To clarify this issue, it is indispensable to reveal what happens to hydrogen defects during high-temperature processes, thereby fingerprinting the origins of hydrogen defects observed in natural orthopyroxene. Here, we carry out Fourier transform infrared spectroscopic studies on hydrogen defects of three natural orthopyroxenes at elevated temperatures to 1000 °C. Hydrogen defects display reversible disordering at temperatures above 700 °C, which is different from those at ambient conditions. Moreover, hydrogen diffusivities are significantly different between the orthopyroxene samples from different tectonic settings despite their similar iron contents. Even for the same crystal, different hydrogen defects display different diffusion behaviors. Hydrogen defects corresponding to the 3420 cm−1 band have the fastest diffusivity relative to the other hydrogen defects. Most importantly, hydrogen defects can redistribute in the crystal, with new hydrogen defects produced at the cost of the initial hydrogen defects rather than involving a reaction with an external hydrogen source. Combining these findings with previously reported hydrogen defects in natural olivine and clinopyroxene at high temperatures, we propose that: (1) to correctly relate hydrogen defects features to geological processes, it is imperative to understand their behavior and origin, and (2) hydrogen disordering should be taken into account when predicting and extrapolating data on physical properties of the mantle from room-temperature measurements.
Influence of multiple uses of water on the sustainability of communally-managed rural water supply systems in Zimbabwe
Authors: Kativhu Tendai , Mazvimavi Dominic , Tevera Daniel , Nhapi Innocent 
Published: December 2020

Abstract
The utilisation of drinking water supply systems for productive uses is not a new practice in Zimbabwe and the world over. This study sought to explore how multiple uses of water, in this case community gardening as a productive use combined with domestic uses influence sustainability of communally-managed rural water supply systems. Using the independent samples t-test, it was noted that community gardening positively influences sustainability. The test was done on institutional, technical, social and financial factors of sustainability. Results showed that there were statistically significant differences in sustainability performance between water points used for multiple uses and those used for domestic uses only. However, it was also noted that using drinking water sources for multiple uses increases the frequency of water use conflicts and water point breakdowns, which negatively impact on sustainability. This means that where water sources are used for multiple uses additional management skills and resources may be required for the water points to be sustained. The study concluded that using domestic water sources for productive purposes enhances sustainability of water supply facilities; however, consideration has to be given about the relative importance and scale of the type of productive activity to be implemented.
Characteristics of rural domestic wastewater with source separation
Authors: Cheng Fangkui , Dai Zheqin , Shen Shuting , Wang Siyu , Lu Xiwu 
Published: December 2020

Abstract
Rural domestic wastewater (RDW), one of the non-point pollution sources, has become a significant object related to sanitation improvement and water pollution control in Taihu Lake Basin, China. Current research on RDW characteristics and management with source separation is limited. In this study, a source-separated investigation into the characteristics of RDW was conducted, and the management suggestions were proposed. The results showed that the average RDW production coefficient was 94.1 ± 31.6 (range: 71.8–143.0) liters per capita (person) per day. Household-level wastewater generation peaked two or three times daily, and the synchronous fluctuation could cause hydraulic loading shocks to treatment facilities. The population equivalents of chemical oxygen demand, ammonium nitrogen (NH4+–N), total nitrogen (TN), and total phosphorus (TP) in RDW were 78.7, 3.7, 4.12, and 0.8 g/(cap·d), respectively. Blackwater from water closet source accounted for 30.4% of the total wastewater amount, contributing 93.0%, 81.7%, and 67.3% to loads of NH4+-N, TN, and TP, respectively. Graywater from the other sources with low nutrient-related pollutant concentrations and loads, accounting for 69.6% of the total wastewater amount, was a considerable alternative water resource. The quantitative and qualitative characteristics indicated that GW and BW had the potential of being reused in relation to water and nutrients, respectively.