bluuebunny/crossref_metadata_2025_split huggingface dataset:
Height predict incident non-alcoholic fatty liver disease among general adult population in Tianjin, China, independent of body mass index, waist circumference, waist-to-height ratio, and metabolic syndrome
Authors: Kumari Shubham , Wang Xuena , Liu Yunyun , Gu Yeqing , Huang Yuhan , Zhang Qing , Liu Li , Meng Ge , Wu Hongmei , Sun Shaomei , Wang Xing , Zhou Ming , Jia Qiyu , Wang Guolin , Song Kun , Niu Kaijun 
Published: January 2020

Abstract
Background : Early-life hormonal and nutritional factors can greatly influence the risk of non-alcoholic fatty liver disease (NAFLD). Adult height is a simple marker for these factors. This study aimed to investigate the association between adult height and NAFLD. Methods : We performed a prospective cohort study of 35,994 participants aged 25 years or over with measured height at baseline. NAFLD was diagnosed by abdominal ultrasound and self-reported history of alcohol intake. Multivariable Cox proportional hazards regression models were conducted to assess the gender-specific association between height and the risk of NAFLD. Results: During a follow-up period of 5.5 years, 6 ,245 of 35,994 subjects developed NAFLD. The adjusted hazard ratios (95% confidence interval) of NAFLD for increasing quintiles of height were 1.00 (reference), 0.82 (0.73, 0.92), 0.84 (0.73, 0.97), 0.72 (0.61, 0.85) and 0.63 (0.50, 0.79) ( P for trend < 0.0001) in males, and 1.00 (reference), 1.00 (reference), 0.80 (0.69, 0.91), 0.72 (0.61, 0.85), 0.60 (0.49, 0.74) and 0.45 (0.35, 0.59) ( P for trend <0.0001) in females, respectively. Conclusions: A higher adult height was associated with lower risk of NAFLD among males and females in Tianjin, China.
Ischemic stroke with a preceding Trans ischemic attack(TIA) less than 24 hours and thrombolytic therapy
Authors: Poupore Nicolas , Strat Dan , Mackey Tristan , Snell Ashley , Nathaniel Thomas 
Published: January 2020

Abstract
Background Acute ischemic stroke attack with and without a recent TIA within or less than 24 hours may differ in clinical risk factors, and this may affect treatment outcomes following thrombolytic therapy. We examined whether the odds of exclusion or inclusion for thrombolytic therapy are greater in ischemic stroke with TIA less than 24 hours preceding ischemic stroke(TIA-24hr-ischemic stroke patients) as compared to those without recent TIA or non-TIA <24 hours. Methods A retrospective hospital-based analysis was conducted on 6,315 ischemic stroke patients, of whom 846 had proven brain diffusion-weighted magnetic resonance imaging (DW-MRI) of an antecedent TIA within 24 hours prior to ischemic stroke. The logistic regression model was developed to generate odds ratios (OR) to determine clinical factors that may increase the likelihood of exclusion or inclusion for thrombolytic therapy. The validity of the model was tested using a Hosmer-Lemeshow test, while the Receiver Operating Curve (ROC) was used to test the sensitivity of our model. Results In TIA-24hr-ischemic stroke population, patients with a history of alcohol abuse (OR = 5.525, 95% CI, 1.003-30.434, p = 0.05), migraine (OR=4.277, 95% CI, 1.095-16.703, p=0.037), and increasing NIHSS score (OR=1.156, 95% CI, 1.058-1.263, p = 0.001) were associated with the increasing odds of receiving rtPA, while older patients (OR = 0.965, 95% CI, 0.934‐0.997, P = 0.033) were associated with the increasing odds of not receiving rtPA. Conclusion In TIA-24hr-ischemic stroke patients, older patients with higher INR values are associated with increasing odds of exclusion from thrombolytic therapy. Our findings demonstrate clinical risks factors that can be targeted to improve the use and eligibility for rtPA in in TIA-24hr-ischemic stroke patients.
A predictive model for treatment response in patients with locally advanced esophageal squamous cell carcinoma after concurrent chemoradiotherapy: based on SUVmean and NLR
Authors: wang chunsheng , Zhao Kewei , Hu Shanliang , Huang Yong , Ma Li , Song Yipeng , Author) Minghuan Li(New Corresponding 
Published: January 2020

Abstract
Background: We conducted this study to combine the mean standardized uptake value (SUVmean) and neutrophil to lymphocyte ratio (NLR) to establish a strong predictive model for patients with esophageal squamous cell carcinoma (ESCC) after concurrent chemoradiotherapy (CCRT).
Methods: We retrospectively analyzed 163 newly diagnosed ESCC patients treated with CCRT. Eighty patients (training set) were randomly selected to generate cut-off SUVmean and NLR values by receiver operating characteristic (ROC) curve analysis and to establish a predictive model by using the independent predictors of treatment outcomes. Then, we evaluated the performance of the prediction model regarding treatment outcomes in the testing set (n=83) and in all sets.
Results: A high SUVmean (>5.81) and high NLR (> 2.42) at diagnosis were associated with unfavorable treatment outcomes in patients with ESCC. The prediction model had a better performance than the simple parameters (p<0.05). With a cut-off value of 0.77, the prediction model significantly improved the specificity and positive predictive value for treatment response (88.9% and 92.1% in the training set, 95.8% and 97.1% in the testing set, and 92.2% and 91.8% in all sets, respectively).
Conclusions: The pretreatment SUVmean and NLR were independent predictors of treatment response in ESCC patients treated with CCRT. The predictive model was constructed based on these two parameters and provides a highly accurate tool for predicting patient outcomes.
Individual and community level factors associated with unmet need for contraception among married reproductive age women in Ethiopia. A multilevel analysis using 2016 Ethiopian Demographic and Health Survey
Authors: Anagaw Melaku Yalew , Desta Bezawit Adane , Abitew Bereket Kefale , Sinishaw Yitayish Damtie 
Published: January 2020

Abstract
Background: There is limited evidence on unmet need to contraceptive among married reproductive age women especially in developing countries like Ethiopia. Thus, this study aimed to assess individual and community level factors associated with unmet need for contraception among married women of reproductive age in Ethiopia, EDHS 2016 dataset, 2019. Method: A secondary analysis was done on Ethiopian Demographic and Health Survey (2016) dataset by using cross sectional study design. A total of 9125 women who were married, fecund and/or sexually active were included in the analysis. Multilevel mixed-effect logistic regression analysis was done by STATA version 14.0 to identify individual and community level factors. Adjusted odds ratio with 95% confidence interval was used to show the strength and direction of association and statistical significance was declared at P value less than 0.05. Result: Factors significantly associated with unmet need were; ages between 15-19 years [AOR=2.25, 95% CI: (1.34, 3.79)], greater than or equal to three living children [AOR=1.87, 95 % CI: (1.40, 2.49)], belong to richer household [AOR=0.73, 95% CI: (0.54, 0.97)], Muslim followers [AOR=1.37, 95% CI: (1.02, 1.83)], married more than once [AOR=1.31, 95 % CI: (1.06, 1.62)]. From community level variables, belong to Somali region [AOR=0.34, 95% CI: (0.19, 0.61)] were significantly associated with unmet need. Conclusion: Both individual and community-level factors were significant determinants of unmet need. From individual level factors; ages of women, number of living children, religion, married more than once and wealth of house hold and from community level variables; region was significantly associated with unmet need for contraception. The findings suggested that health care providers should mainly focus on women nearly on menopauses, who live in the poorest household and who had many number of living children and married more than once to decrease unmet need to contraceptive.
Development of A Guideline for Reporting Mediation Analyses (AGReMA)
Authors: Author) Aidan G Cashin(Former Corresponding , McAuley James H , Lamb Sarah E , Hopewell Sally , Kamper Steven J , Williams Christopher M , Henschke Nicholas , Author) Hopin Lee(New Corresponding 
Published: January 2020

Abstract
Background There are a growing number of studies using mediation analysis to understand the mechanisms of health interventions and exposures. Recent work has shown that the reporting of these studies is heterogenous and incomplete. This problem stifles clinical application, reproducibility, and evidence synthesis. This paper describes the processes and methods that will be used to develop a guideline for reporting studies of mediation analyses (AGReMA). Methods/Design AGReMA will be developed over five overlapping stages. Stage one will comprise a systematic review to examine relevant evidence on the quality of reporting in published studies that use mediation analysis. In the second stage we will consult a group of methodologists and applied researchers by using a Delphi process to identify items that should be considered for inclusion in AGReMA. The third stage will involve a consensus meeting to consolidate and prioritise key items to be included in AGReMA. The fourth stage will involve production of AGReMA and an accompanying explanation and elaboration document. In the final stage we will disseminate the AGReMA statement via journals, conferences, and professional meetings across multiple disciplines. Discussion The development and implementation of AGReMA will improve the standardization, transparency, and completeness in the reporting of studies that use mediation analysis to understand the mechanisms of health interventions and exposures.
Allo-HSCT compared with immunosuppressive therapy for acquired aplastic anemia: Is superiority a one-sided understanding?
Authors: Zhu Yangmin , Gao Qingyan , Hu Jing , Guan Dongrui , Liu Xu , Zhang Fengkui 
Published: January 2020

Abstract
Acquired aplastic anemia (AA) is a rare hematologic disease characterized by a profound pancytopenia and hypocellular bone marrow. To comprehensively compare the efﬁcacy and safety of allogeneic hematopoietic stem cell transplantation (allo-HSCT) with immunosuppressive therapy (IST) as a front-line treatment for patients with AA. We searched the Medline, Embase, and Cochrane Registry of Controlled Trials databases from January 2000 to March 2019. Studies comparing allo-HSCT with IST as a first-line therapy for patients with AA were included. Fifteen studies including a total of 5336 patients were included in the meta-analysis. The pooled hazard ratio (HR) for overall survival (OS) was 0.4 (95% CI 0.074–0.733, P = 0.016, I2 = 58.8%) and the pooled HR for failure-free survival (FFS) was 1.962 (95% CI 1.43–2.493, P = 0.000, I2 = 0%). The pooled relative risk (RR) for overall response rate (ORR) was 1.691 (95% CI 1.433–1.996, P = 0.000, I2 = 11.6%). Although survival was significantly longer among AA patients undergoing first-line allo-HSCT compared to those undergoing first-line IST, the selection of initial treatment for patients with newly diagnosed AA still requires comprehensive evaluation of donor availability, patient age, expected quality of life, risk of disease relapse or clonal evolution after IST, and potential use of adjunctive eltrombopag.
Outcome of intravitreal bevacizumab injection without pre and postoperative antibiotics
Authors: Shrestha Ruchi , Karki Pratap , Joshi Sagun Narayan 
Published: January 2020

Abstract
Backgound Intravitreal injections are the most common treatment modality for several retinal pathologies. Despite endophthalmitis being the most feared complication, antibioprophylaxis remains controversial in intravitreal injections.Methods This was a retrospective study done for a period of 2 years from 1st January 2017 to 31st December 2018 in BPKLCOS among patients receiving intravitreal bevacizumab. The intravitreal injection was given by a single surgeon. It included 503 eyes which received intravitreal bevacizumab over a period of 2 years without pre and postoperative antibiotics.Results Out of 503 eyes studied over a period of 2 years without antibiotic prophylaxis the rate of endophthalmitis was 0.0019% which is very low compared to the other studies with rate of endophthalmitis between 0.019-0.09%.Conclusion The rate of endophthalmitis doesn’t increase after giving intravitreal injections without the use of preoperative/postoperative prophylactic antibiotics. Intravitreal injection can be given safely without pre-operative and post-operative antibiotics. Trial Registration not applicable as it is a retrospective study.
In vitro transcription and micro-injection of Kdm4a mRNA into mouse oocytes
Authors: SANKAR ADITYA 
Published: January 2020

Abstract
This experiment describes the in vitro transcription of Kdm4a wildtype and H188A catalytic dead mRNA. This details also its subsequent injection into mouse oocytes followed my IVF to track the impact on embryo development. The procedure is technically challenging and performed by the Transgenic Core Facility at the University of Copenhagen. Oocytes have a poorer survival rate following mRNA inject as against zygotes. However the objective was to demonstrate the earliest stage of intervention to rescue developmental failure of KDM4A maternal zygotic mutant embryos
ChIP-Seq data processing, normalization and visualization
Authors: SANKAR ADITYA , Lerdrup Mads 
Published: January 2020

Abstract
This article explains the step-by-step processing of NGS data of ChIPSeq samples for H3K4me3, H3K9me3 and H3K36me3 immunoprecipitations performed in oocytes/embryos. This includes sections on visualization of data, and a comment on normalization.the associated raw data have been deposited at GSE129735 on Gene Expression Omnibus.
Single oocyte/embryo RNASeq data processing
Authors: SANKAR ADITYA , Johansen Jens Vilstrup 
Published: January 2020

Abstract
This procotol details the step-by-step procedures followed to process the single oocyte/embryo mRNASeq and 2-cell embryo Total RNASeq data generated using the SMARTSeq2 technology in the associated publication.
Comparison of power-free-chop and phaco-chop techniques for moderate nucleus
Authors: Bai Haiqing , Yao Lin 
Published: January 2020

Abstract
Background: To compare intraoperative and postoperative effects of power-free-chop and phaco-chop techniques for moderate nucleus in phacoemulsification sugery. Methods: Sixty patients were evaluated in 2 groups prospectively. The power-free-chop technique was performed in Group 1 (30 eyes) and the phaco-chop technique in Group 2 (30 eyes). There were no significant differerces between these 2 groups. The cumulative dissipated energy (CDE), time to achieve maximum vision, corneal thickness variation, and time to return to the preoperative values were collected. All parameters were statistically compared in these 2 groups by using the chi-square test and the independent-sample t test. Results: The CDE was 5.53±1.92J in Group 1 and 7.02±1.77J in Group 2. After the operation, the mean time to recover to the maximum vision was 2.80±1.42 days in Group 1 and 3.80±1.92 days in Group 2 respectively. The mean postoperative corneal thickness increased 36.9±14.74μm in Group1 and 46.20±20.67μm in Group 2. And the mean time to return to preoperative pachymetry values were 3.73±1.70 days and 4.83±2.11 days, in Group 1 and Group 2 respectively. There were significant differences in these parameters between both groups. Conclusions: The power-free-chop technique had fewer negative effects on the corneal endothelium as less ultrasound power was used for moderate nucleus cases. This can accelerate the functional healing process and the return to preoperative physiologic values.
Long-term ambient air pollutant exposure and risk of migraine and recurrent headache in children: A 12-year cohort study
Authors: Hong Syuan-Yu , Wan Lei , Lin Hui-Ju , Lin Cheng-Li , Wei Chang-Ching 
Published: January 2020

Abstract
Background Although research has suggested environmental factors to be triggers of headache, the contribution of long-term air pollution exposure to migraine and recurrent headaches (migraine/headaches) is poorly understood. Hence, we executed this nationwide cohort study to investigate the association of levels of ambient air pollution with the incidence and the risk of migraine/headaches in Taiwan children from 2000 to 2012. Methods We collected data from the Taiwan National Health Insurance Research Database and linked them to the Taiwan Air Quality Monitoring Database. Overall 218,008 children aged <18 (0-17) years old were identified from January 1, 2000 and then followed until they were diagnosed by a physician >=3 times with migraine/headaches or until December 31, 2012. We categorized the annual average concentration of each air pollutant (fine particulate matter, total hydrocarbon, methane, sulfur dioxide, and nitrogen dioxide) into quartiles (Q1-Q4). We measured the incidence rate, hazard ratios (HRs), and the corresponding 95% confidence intervals for migraine/headaches stratified by the quartiles. Results A total of 28037 children (12.9%) were identified with migraine/headaches. The incidence rate and adjusted HR for migraine/headaches increased with higher-level exposure of air pollutants, except sulfur dioxide. Conclusions We herein demonstrate that long-term ambient air pollutant exposure might be a risk factor for childhood migraine/headaches.
Physical activity and 4-year changes in body weight in 52,498 non-obese people: The Lifelines Cohort
Authors: Byambasukh Oyuntugs , Vinke Petra , Kromhout Daan , Navis Gerjan , Corpeleijn Eva 
Published: January 2020

Abstract
Objectives: We investigated associations between daily-life physical activity (PA) and prospective weight gain in non-obese people. We also examined whether these associations were independent of other lifestyle factors and changes in muscle mass and whether they were age-dependent and changed over a person’s life course. Methods: The data were extracted from the Lifelines cohort study (N=52,498; 43.5% men) and excluded obese individuals (BMI>30kg/m 2 ). We used the validated SQUASH questionnaire to estimate moderate-to-vigorous (MVPA; MET≥4), moderate (MPA; MET between 4 and 6.5) and vigorous PA (VPA; MET≥6.5) within non-occupational (commuting and leisure) and occupational domains. Body weight was objectively measured and changes were standardized to a 4-year period. Separate analyses, adjusted for age, educational level, diet, smoking, alcohol consumption and changes in creatinine excretion (a marker of muscle mass), were performed for men and women. Results: The average weight gain was +0.45±0.03 kg in women. Relative to each reference groups (No-MVPA, No-MPA and No-VPA), non-occupational MVPA (Beta (95%CI): -0.34 kg (-0.56;-0.13)), MPA (-0.32 kg (-0.54;-0.10)) and VPA (-0.30 kg (-0.43;-0.18)) were associated with less gain in body weight in women after adjusting for potential confounders, described above. These associations were dose-dependent when physically active individuals were divided in tertiles. Beta-coefficients (95%CI) for the lowest, middle, and highest MVPA tertiles relative to the ‘No-MVPA’ were, respectively, -0.24 (-0.47;-0.02), -0.31 (-0.53;-0.08), and -0.38 (-0.61;-0.16) kg. The average weight gain in men was +0.13±0.03 kg, and only non-occupational VPA was associated with less body weight gain. Beta-coefficients (95%CI) for the VPA tertiles relative to the ‘No-VPA’ group were, respectively, -0.25 (-0.42;-0.09), -0.19 (-0.38;-0.01) and -0.20 (-0.38;-0.02) kg. However, after adjusting for potential confounders, the association was no longer significant in men. The potential benefits of non-occupational PA were age-stratified and mainly observed in younger adults (men: <35 years; women: <55 years). Moreover, occupational MVPA was not associated with favourable changes in body weight in males and females. Conclusion: Higher non-occupational MVPA, MPA, and VPA were associated with less weight gain in women <55 years. In younger men (<35 years), only non-occupational VPA was associated with less weight gain.
Intrathecal morphine pretreatment ameliorated hepatic ischemia-reperfusion injury by reducing inflammation through peripheral and spinal opioid activation in rats
Authors: Wang Yuan , Liu Jueying , Qu Yifeng , Fan Yinghui , Yu Weifeng 
Published: January 2020

Abstract
Background Hepatic ischemia-reperfusion injury (IRI) is a common phenomenon after liver transplantation and liver tumor surgery. Morphine provides analgesia and prevents hepatic IRI. Besides morphine in the spinal cord has the protective effect on hepatic IRI. But the mechanism has not been well characterized. This study investigated whether morphine administrated intrathecally mimic the protective effect by reducing inflammation, and whether this kind of effect was mediated by phosphatidylinositol-3-kinase (PI3K)/Akt pathway. Methods We established models of hepatic IRI and intrathel catheter insertion on Sprague-Dawley. Morphine was administrated 10min prior hepatic ischemia flowed with 6h reperfusion. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), histological changes and liver cell apoptosis assessed liver IRI. Similar maneuvers were repeated using the optimal dose and opioid receptor antagonist naloxone methiodide (NM). Serum ALT and AST, histological staining, hepatic apoptosis and serum levels of tumor necrosis factor alpha (TNF-α) and interleukin 1β (IL-1β) were assessed. Expression of Akt and its downstream protein Erk were evaluated by Western blotting. Results Morphine pretreatment intrathecally at dose of 10μg·mg -1 reduced ALT and AST levels, hepatic apoptosis, inflammation and preserved liver structure. These changes were partially reserved by NM 10min prior morphine injection. Morphine elevated the expression of Akt and Erk, however, NM did not decrease Akt and Erk expression. Conclusion Intrathecal morphine pretreatment reduced rats’ livers’ inflammation through both peripheral and spinal opioid receptors activation. The potential mechanism contributed to moderate PI3K/Akt pathway.
Improving Ruminal Degradibility Of Oil Palm Fronds Using Enzyme Extracts From White Rot Fungi
Authors: Nicholas Ahmad Fariz - , Hassim H.A. - , Nicholas A.F. - , Lourenço Marta - , Dias A.A - , Goh Y.M. , Fievez V. - 
Published: January 2020

Abstract
Background: Oil palm fronds (OPF) when pretreated with white rot fungi (WRF) shows increased rumen degradability but with significant biomass loss. Thus, effects of pre-treated OPF with enzyme extracts from WRF on rumen degradability were studied in vitro. The enzyme extracts were prepared by inoculating OPF with three WRF, i.e Ceriporiopsis subvermispora, Lentinula edodes and Ganoderma lucidum, for 15, 30 and 45 days with either ammonium sulphate, (NH4)2SO4 and sodium nitrate, NaNO3 added to the culture media for each inoculation period. After preparation of enzyme extracts, the enzyme activities were determined. OPF was then pre-treated with enzyme extracts in a citrate buffer (pH 5.0) in a forced air oven at 40 oC during 5 days. Further, the in vitro rumen degradation of OPF pre-treated with enzyme extracts, with respect to the short chain fatty acid (SCFA) production, was determined after 24 h incubation. Activity of lignolytic (laccase and MnP), cellulolytic (CMCase and avicelase) and hemicellulolytic (xylanase) enzymes were measured in all of the extracts irrespective on the inoculation period. Results: Treatment of OPF with enzyme extracts from G. lucidum after 45 days of inoculation showed a numerical increase (13%) in total SCFA and apparently rumen degradable carbohydrates (ARDC) after 24 h in vitro incubation, without any loss of biomass. However, this increase was not clearly correlated to results of the enzyme assays. Conclusion: This study indicates pre-treatment of OPF with enzyme extracts from specific WRF to be promising to enhance the ruminal degradability of OPF without simultaneous loss of biomass.