bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oriented Perforation using Tubing Conveyed Perforating TCP Gun with Dynamic Underbalance DUB as Sand Control Method in Low Angle Wells, Offshore Sarawak, Malaysia.
Authors: A Rahman Adib Mahfuz , Bakar Hasmizah , Daly Kerry Gordon , Naharindra Adhi , Kumar Suman , Baharuddin Nurbaiti , Ibrahim Ahmed Abdelatty 
Published: October 2019

Abstract
The use of oriented perforation as a means of sand control technique has been adopted by many operators in industry. Two low angle wells completed in D field offshore Sarawak, Malaysia with moderate to weak rock strength quality required this method not only to limit the sand production but also to sustain the minimum production of 2500bopd each. Based on onset sand production analysis, reservoir M is predicted to have sand production in the direction of a maximum stress of 135degrees from true north. The wells then are suggested to be shot 0-180degree parallel to maximum stress and it saved the expense of no installation of downhole sand exclusion. This paper outlines the perforation analysis conducted to design the optimum perforation using Tubing Conveyed Perforating (TCP) Gun with Dynamic Underbalance (DUB) that effectively clean the newly created perforations. The operational approach applied to overcome the challenge of achieving desired orientation in almost vertical deviation wells by positioning electronic gyroscope siting on Universal Bottom Hole Orientation (UBHO) sub is also discussed in this paper. The available centralization solutions in market are limited to 30° degrees well inclination with 5°accuracies at three times more cost than conventional TCP. The proposal of oriented perforation at both slanted wells significantly saved lot of project economic and proved as effective sand control method, so far the production target is achieved and maintained with no record of sand production at field.
Two newly drilled and completed wells located in offshore Sarawak, Malaysia required oriented perforation with dynamic underbalance using Tubing Conveyed Perforating (TCP) guns. The reservoir quality was weak to moderate rock strength with expected sand production in the direction of a maximum stress (135 degrees from true north) within the nearly vertical wellbore. To limit the sand production, the requirement was to shoot 0-180 degree guns parallel to maximum stress, to save the expense of any sand exclusion method. Another requirement was to use Dynamic Underbalance (DUB) to properly clean perforations, reduce skin, and maximize production. The objective was to achieve a minimum production of 2500 BPD.
From Geo-mechanic study, in deviated well, the orientation of perforation is aligned with the trend of maximum horizontal stress. Based on breakout analysis, Shmax direction is 135° from the North. The expected production rate can be achieved with Oriented Perforation even at 0 deg phasing and PI reduced from 55 STB/day/psi to 38 STB/day/psi based on Figure 1 below. The studies conducted on Field D available data concluded that sanding risk is very low with Cased & Oriented Perforation strategy.
The association between symptoms of depression during pregnancy and low birth weight:a prospective study
Authors: Li Xiu xiu , Gao Rui , Dai Xiaowei , Liu Hong , Zhang Jinxin , Liu Xuemei , Si Dongmei , Deng Te , Xia Wei 
Published: October 2019

Abstract
Abstract
Objectives
Most studies have showed that maternal depression is associated with pregnancy complications. However, there were limited evidences in Chinese population. We examined the associations of the antenatal depression symptoms with pregnancy outcomes, especially for low birth weight.
Methods
A total of 1377 singleton pregnant women were recruited from Nanshan Maternity & Child Healthcare Hospital of Shenzhen in this prospective cohort study. Depression symptoms were assessed by the Edinburgh postnatal depression scale (EPDS) questionnaire in the second trimester of gestation; cut-points for the indication of antenatal depression were ≥12 scores in this study. Sociodemographic data, life-style and pregnancy outcomes were collected through Shenzhen Maternity & Child Healthcare database. The risks of adverse outcomes in patients with depression were determined by multivariate logistic regression and represented as odds ratios (ORs) and 95% confidence intervals (CIs).
Results
Of the 1377 subjects, the prevalence of antenatal depression was 19.1%. The EPDS scores were 13.8±2.0 and 6.5±2.9 ( P <0.001) in subjects with and without antenatal depression, respectively. After adjustment for maternal age, education, parity, pre-pregnancy body mass index (BMI), residential area, fetal gender, an EPDS score≥12 (versus. <12) was associated with an increased risk for low birth weight (odds ratio: 2.05, 95% CI: 1.12-4.64), but not for preterm birth, large for gestational age, small for gestational age and macrosomia.
Conclusion Pregnant women presenting antenatal depressive symptoms are at elevated risk of low birth weight. Mental health problems of pregnancy should be addressed for the prevention of low birth weight.
Potential for non-combustible nicotine products to reduce socioeconomic inequalities in smoking: a systematic review and synthesis of best available evidence
Authors: Lucherini Mark , Smith Katherine , Hill Sarah 
Published: October 2019

Abstract
Background: While some experts have emphasised the potential for e-cigarettes to facilitate cessation among smokers with low socioeconomic status (SES), there is limited evidence of their likely equity impact . We assessed the potential for electronic cigarettes and other non-combustible nicotine-containing products (NCNPs) to reduce inequalities in smoking by systematically reviewing evidence on their use by SES in countries at stage IV of the cigarette epidemic.
Methods: Ten electronic databases were searched in February 2017 using terms relating to e-cigarettes, smokeless tobacco and nicotine replacement therapy (NRT); and SES. We included studies published since 1980 that were available in English and examined product use by SES indicators such as income and education. Data synthesis was based on those studies judged to be of medium- to high-quality using guidelines adapted from the Critical Appraisal Skills Programme.
Results: We identified 54 studies describing NCNP use by SES across 12 countries, of which 27 were judged of sufficient quality to include in data synthesis. We found mixed patterns of e-cigarette current use by SES, with evidence of higher use among low-income adults but unclear or mixed findings by education and occupation. In contrast, smokeless tobacco current use was consistently higher among low SES adults. There was very limited evidence on the SES distribution of NRT in adults and of all NCNPs in young people.
Conclusions: The only NCNP for which there are clear patterns of use by SES is smokeless tobacco, where prevalence is higher among low SES groups. While this suggests a potentially positive impact on inequalities in smoking (if NCNP use displaces smoked tobacco use), this has not been seen in practice. These findings do not support the suggestion that e-cigarettes have the potential to reduce social inequalities in smoking, since i) current evidence does not show a clear trend of higher e-cigarette use in population groups with higher tobacco consumption, and ii) the experience of smokeless tobacco suggests that – even where NCNP use is higher among low SES groups – this does not necessarily replace smoked tobacco use in these groups.
Digital anatomical study of screw placement for quadrilateral plate through the middle window of ilioinguinal approach
Authors: Zhao Bei , Mu Weidong 
Published: October 2019

Abstract
Background Screw placement directly for quadrilateral plate fractures of the acetabulum is very difficult. This study was performed to simulate the surgical procedure and try to obtain effective and safe screw angles through the middle window of ilioinguinal approach in Chinese patients. Methods We randomly collected the pelvic computed tomography (CT) scans of 50 adults. DICOM-formatted CT-scan images were imported into Mimics software. The three-dimensional reconstruction (3D) digital model of the semi-pelvic was established. In the coronal and sagittal planes, a 3.5 mm cylinder was used to simulate the pathway of the screw from the designated insertion point. The angles of insertion and intersex differences were explored by statistical analyses. Results The screws could be inserted via four angles: medial inclination, lateral inclination, anterior inclination and posterior inclination. The mean minimum medial inclination angle (MIMIA) of insertion point A was 4.96°±1.11° in males and 8.66°±3.40° in females, and the intersex difference was significant. The mean minimum medial inclination angle (MIMIA) of insertion point C was -5.31°±3.69° in males and 1.75°±8.95° in females, and the intersex difference was significant. There were no differences in all the angles between males and females in insertion point B. Conclusions Preoperative measurement and calculation by digital tools before the screw placement for quadrilateral plate fractures of the acetabulum are feasible. Double cortical screws could be placed safely through the middle window of ilioinguinal approach to increase the stability of acetabulum.
Growth Inhibitory Effect of Fibroblast Growth Factor Peptide Antagonist on Mouse Model Breast Tumor through ERK/MAPK and PI3K/AKT Signaling Pathways
Authors: jafarzadeh mehrzad , Mousavizadeh Kazem , Bahremani Mohammad hashemi , Asghari S.mohsen 
Published: October 2019

Abstract
Background In the majority of cancers, metastasis of tumor cells is main cause of treatment failure. The current research investigated the effectiveness basic fibroblast growth factor (bFGF) peptide developed to inhibit tumor growth in 4T1 metastatic breast cancer through the PI3K/AKT and ERK/MAPK signal transduction pathways. In BALB/c mice, the tumor was induced through 4T1 tumor graft. Administration of the peptide was done one time a day at various doses of 1, 2.5, or 10 mg/kg over 14 days. After two weeks, the designed peptide injected .After the treatment period, the mice underwent surgery, and tumors were used for the Immunohistochemistry, Apoptosis, and western blot examinations.Results Based on one-way ANOVA and p ≤0.05, findings showed that the P-AKT and p-ERK contents in tumors treated with peptide reduced in two mentioned signaling pathways and the peptide injection were effective in reducing or inhibiting tumor growth.Conclusion Our findings showed that in the two signal transduction pathways, the P-AKT and p-ERK levels were significantly different from the negative control group.
Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia
Authors: Amalina Nor Zulkiply , Santha Silvaraj , Zulperi Dzarifah , Amal MNA , Yusof Mohd Termizi , Zamri-Saad Mohd , Ina-Salwany Md Yasin 
Published: October 2019

Abstract
Background: Numerous prevalence studies of Vibrio spp. infection in fish have been extensively reported worldwide, including Malaysia. Unfortunately, information on the prevalence of Vibrio spp. in groupers (Epinephelus spp.) is limited. In this study, groupers obtained from nine farms located at different geographical regions in Malaysia were sampled for the presence of pathogenic Vibrio spp. and their susceptibility profiles against seven antibiotics. Results: Out of 270 grouper samples, 195 (72%) were detected with the presence of Vibrio spp. Vibrio communis showed highest prevalence in grouper (28%), followed by V. parahaemolyticus (25%), V. alginolyticus (19%), V. vulnificus (14%), V. rotiferianus (3%), Vibrio sp. (3%), V. campbellii (2%), V. mytili (2%), V. furnissii (2%), V. harveyi (1%), V. tubiashii (1%), V. fluvialis (0.3%) and V. diabolicus (0.3%). Assessment on the antibiotic susceptibility profiles of the Vibrio spp. revealed that majority of the isolates were susceptible to tetracycline, streptomycin, erythromycin and bacitracin, but resistance to ampicillin, penicillin G and vancomycin. The mean MAR index of the Vibrio isolates was 0.51, with 85% of the isolates showed MAR index value of higher than 0.2. Results indicate that the Vibrio spp. were continuously exposed to antibiotics. Furthermore, the plasmid profiles of Vibrio spp. showed that 38.7% of the isolates harbored plasmid with molecular weight of more than 10 kb, while 61.3% were without plasmid. During curing process, Vibrio spp. lost their plasmid, but remained resistant to ampicillin, penicillin G, bacitracin and vancomycin while a few isolates remained resistant to erythromycin, streptomycin and tetracycline.The results suggested that the resistance to antibiotics in isolated Vibrio spp. might be due to chromosomal and plasmid borne. Conclusions: This study demonstrates the prevalence of Vibrio spp. in groupers and the distribution of multidrug resistance strains that could be of concern to the farmers in Malaysia. In addition, data from this study can be further used in fish disease management plan.
Genome-wide identification of diacylglycerol acyltransferases (DGAT) family genes influencing milk production traits in buffalo
Authors: Liu Jiajia , Wang Zhiquan , Li Jun , Li Hui , Yang Liguo 
Published: October 2019

Abstract
Background: The diacylglycerol acyltransferases (DGAT) are a vital group of enzymes in catalyzing triacylglycerol biosynthesis. DGAT genes like DGAT1 and DGAT2 , have been identified as two functional candidate genes affecting milk production traits, especially for fat content in milk. Buffalo milk is famous for its excellent quality, which is rich in fat and protein content. Therefore, this study aimed to characterize DGAT family genes in buffalo and to find candidate markers or DGAT genes influencing lactation performance. Results: We performed a genome-wide detection and identified eight DGAT genes in buffalo. All the DGAT genes classified into two distinct clades (DGAT1 and DGAT2 subfamily) based on their phylogenetic relationships and structural features. Chromosome localization displayed eight buffalo DGAT genes distributed on five chromosomes. Collinearity analysis revealed that the DGAT family genes were extensive homologous between buffalo and cattle. Afterward, we discovered genetic variants loci within the genomic regions that DGAT genes located in buffalo. Eight haplotype blocks were constructed and were associated with buffalo milk production traits. Single marker association analyses revealed four most significant single nucleotide polymorphisms (SNPs) mainly affecting milk protein percentage or milk fat yield in buffalo. Genes functional analysis indicated that these DGAT family genes could influence lactation performance in the mammal through regulating lipid metabolism. Conclusion: In the present study, we performed a comprehensive analysis for the DGAT family genes in buffalo, which including identification, structural characterization, phylogenetic classification, chromosomal distribution, collinearity analysis, association analysis, and functional analysis. These findings provide useful information for an in-depth study to determine the role of DGAT family gens play in the regulation of milk production and milk quality improvement in buffalo. Keywords: DGAT family, DGAT1, DGAT2 , Milk production traits, Buffalo genome
Rapid genomic DNA variation in newly hybridized carp lineages derived from Cyprinus carpio (♀) × Megalobrama amblycephala (♂)
Authors: Luo Kaikun , Wang Shi , Fu Yeqing , Zhou Pei , Huang Xuexue , Gu Qianhong , Li Wuhui , Wang Yude , Hu Fangzhou , Liu Shaojun 
Published: October 2019

Abstract
Background: Distant hybridization can generate changes in phenotypes and genotypes that lead to the formation of new hybrid lineages with genetic variation. In this study, based on the establishment of two bisexual fertile carp lineages, including the improved diploid common carp (IDC) lineage and the improved diploid scattered mirror carp (IDMC) lineage, from the interspecific hybridization of common carp (Cyprinus carpio, 2n = 100) (♀) × blunt snout bream (Megalobrama amblycephala, 2n = 48) (♂), provided a good platform to investigate the relationship of genetic and variation between the parents and their hybrid progenies. Result: In this study, we investigated the genetic variation of 12 Hox genes in the two types of carp lineages derived from common carp (♀) × blunt snout bream (♂). Hox gene clusters were abundant in the first generation of IDC, but most were not stably inherited in the second generation. In contrast, we did not find obvious mutations in Hox genes in the first generation of IDMC, and almost all the Hox gene clusters were stably inherited from the first generation to the second generation of IDMC. Interestingly, we found obvious recombinant clusters of Hox genes in both carp lineages, and partially recombinant clusters of Hox genes were stably inherited from the first generation to the second generation in both types of carp lineages. On the other hand, some Hox genes were gradually becoming pseudogenes, and some genes were completely pseudogenised in IDC or IDMC. Conclusions: Our results provided important evidence that distant hybridization produces rapid genomic DNA changes that may or may not be stably inherited, providing novel insights into the function of hybridization in the establishment of improved lineages used as new fish resources for aquaculture.
Shedding light: A phylotranscriptomic perspective illuminates the origin of photosymbiosis in marine bivalves
Authors: Li Jingchun , Lemer Sarah , Kirkendale Lisa , Bieler Rüdiger , Cavanaugh Colleen , Giribet Gonzalo 
Published: October 2019

Abstract
Background Photosymbiotic associations between metazoan hosts and photosynthetic dinoflagellates are crucial to the trophic and structural integrity of many marine ecosystems, including coral reefs. Although extensive efforts have been devoted to study the short-term ecological interactions between coral hosts and their symbionts, long-term evolutionary dynamics of photosymbiosis in many marine animals are not well understood. Within Bivalvia, the second largest class of mollusks, obligate photosymbiosis is found in two marine lineages: the giant clams (subfamily Tridacninae) and the heart cockles (subfamily Fraginae), both in the family Cardiidae. Morphologically, giant clams show relatively conservative shell forms whereas photosymbiotic fragines exhibit a diverse suite of anatomical adaptations including flattened shells, leafy mantle extensions, and lens-like microstructural structures. To date, the phylogenetic relationships between these two subfamilies remain poorly resolved, and it is unclear whether photosymbiosis in cardiids originated once or twice.Results In this study, we establish a backbone phylogeny for Cardiidae utilizing RNASeq-based transcriptomic data from Tridacninae, Fraginae, and other cardiids. A variety of phylogenomic approaches were used to infer the relationship between the two groups. Our analyses found conflicting gene signals and potential rapid divergence among the lineages. Overall, results support a sister group relationship between Tridacninae and Fraginae, which diverged during the Cretaceous. Although a sister group relationship is recovered, ancestral state reconstruction using maximum likelihood-based methods reveals two independent origins of photosymbiosis, one at the base of Tridacninae and the other within a symbiotic Fraginae clade.Conclusions The newly revealed common ancestry between Tridacninae and Fraginae brings a possibility that certain genetic, metabolic, and/or anatomical exadaptation existed in their last common ancestor, which promoted both lineages to independently establish photosymbiosis, possibly in response to the modern expansion of reef habitats.
A novel insight into nitrogen and auxin signaling in lateral root formation in tea plant [Camellia sinensis (L.) O. Kuntze]
Authors: Hu Shunkai , Yang Yiqing , Zhang Mi , Xuan Wei , Zou Zhongwei , Arkorful Emmanuel , Chen Yi , Ma Qingping , Jeyaraj Anburaj , Chen Xuan , Li Xinghui 
Published: October 2019

Abstract
Lateral roots (LRs) are the main organ for tea plant to absorb soil moisture and nutrients. Tea plant (Camellia sinensis) is one of the most popular non-alcoholic beverage worldwide. LRs formation and development are limited by the nitrogen and auxin signaling pathway. In order to understand the function of auxin and nitrogen in LRs formation and development, transcriptome analysis was applied to investigate the differentially expressed genes involved in LRs of tea plant treated with indole-3-butyric acid (IBA), N-1-naphthylphthalamic acid (NPA), low and high nitrogen concentration. A total of 296 common differentially expressed genes were mainly identified and annotated to four signaling pathways, such as nitrogen metabolism, plant hormone signal transduction, Glutathione metabolism and transcription factors. RNA-sequencing results revealed that majority of differentially expressed genes play important roles in nitrogen metabolism and hormonal signal transduction. Low nitrogen condition induced the biosynthesis of auxin and accumulation of genes expression; thus, regulated lateral roots formation. Furthermore, metabolisms of cytokinin and ethylene biosynthesis were also involved in lateral roots development. Transcription factors like MYB genes also contributed to the lateral roots formation of tea plants through secondary cell wall biosynthesis. Reversed phase ultra performance liquid chromatography (RP-UPLC) results showed that the auxin concentration in lateral roots was increased, while the nitrogen level decreased. Thus, tea plant LRs formation could be induced by low nitrogen concentration via auxin biosynthesis and accumulation. This study provides new insights into the mechanisms associated with nitrogen-auxin signaling pathways to regulate LRs formation and arises new clues for the efficient utilization of nitrogen in tea plant at the genetic level
Absence of reproduction-immunity trade-off in male Drosophila melanogaster evolving under differential sexual selection
Authors: Syed Zeeshan Ali , Arun Manas Geeta , Gupta Vanika , Dhiman Aatashi , Nandy Bodhisatta , Prasad Nagaraj Guru 
Published: October 2019

Abstract
Background: The theory of trade-off suggests that limited resources should lead to trade-off in resource intensive traits such as, immunity related and sexually selected traits in males. Alternatively, sexual exaggerations can also act as an honest indicator of underlying immunocompetence, leading to positive correlations between these traits. Evidences in support of either hypothesis in invertebrates is equivocal. Whereas several studies have addressed this question, few have used naturally occurring pathogens and realized post infection realized immunity (e.g., survivorship) to assay the fitness correlations between these two sets of traits.
Results: Adopting an experimental evolution approach, we evolved replicate populations of Drosophila melanogaster under differential sexual selection (high and low) regimes for over a hundred generations and found the following in virgin and mated males in three separate assays:
Post infection survivorship against two natural pathogens - Pseudomonas entomophila (Pe) and Staphylococcus succinus (Ss): Mated males survived better against Pe, but were no different than virgins against Ss.

Bacterial clearance ability against a third natural pathogen Providencia rettgeri (Pr): Mated males had significantly lower CFUs than virgins.

However, sexual selection history had no effect on realized immunity of either virgin or mated males.
Conclusion: We show that while mating can affect realized immunity in a pathogen specific way, sexual selection did not affect the same. The results highlight that complex polygenic traits such as immunity and reproductive traits not necessarily evolve following a binary trade-off model. We also stress the importance natural pathogens while studying sexual selection-immunity correlations.