bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation on the Diagnostic Efficiency of Different Methods in Detecting COVID-19.
Authors: Yang Haitao , Lan Yuzhu , Yao Xiujuan , Lin Sheng , Xie Baosong 
Published: November 2020

Abstract
Objective: To evaluate the diagnostic efficiency of different methods in detecting COVID-19.Methods: PubMed, Web of Science and Embase databases were searched for identifing eligible articles. All data were calculated utilizing Meta Disc 1.4, Revman 5.3.2 and Stata 12. The diagnostic efficiency was assessed via these indicators including summary sensitivity and specificity, positive likelihood ratio (PLR), negative LR (NLR), diagnostic odds ratio (DOR), summary receiver operating characteristic curve (sROC) and calculate the AUC. Results: 18 articles (3648 cases) were included. EPlex: pooled sensitivity was 0.94; specificity 1.0; PLR 90.91; NLR 0.07; DOR 1409.49; AUC=0.9979, Q*=0.9840. Panther Fusion: pooled sensitivity was 0.99; specificity 0.98; PLR 42.46; NLR 0.02; DOR 2300.38; AUC=0.9970, Q*=0.9799. Simplexa: pooled sensitivity was 1.0; specificity 0.97; PLR 26.67; NLR 0.01; DOR 3100.93; AUC=0.9970, Q*=0.9800. Cobas®: pooled sensitivity was 0.99; specificity 0.96; PLR 37.82; NLR 0.02; DOR 3754.05; AUC=0.9973, Q*=0.9810. RT-LAMP: pooled sensitivity was 0.98; specificity 0.99; PLR 36.22; NLR 0.04; DOR 751.24; AUC=0.9905, Q*=0.9596. Xpert Xpress: pooled sensitivity was 0.99; specificity 0.97; PLR 27.44; NLR 0.01; DOR 3488.15; AUC=0.9977, Q*=0.9829.Conclusions: These methods (ePlex, Panther Fusion, Simplexa, Cobas®, RT-LAMP and Xpert Xpress) bear higher sensitivity and specificity, and might be efficient methods complement to the gold standard.
Residual Malaria Among Migrant Workers in Myanmar: Why Still Persistent and How to Eliminate?&nbsp;
Authors: Nyunt Myat Htut , Aye Khin Myo , Naing Shine Thura , Mon Aye Su , Htwe Mi Mi , Win Su Mon , Thwe Wai Myat , Thi Aung , Kyaw Myat Phone 
Published: November 2020

Abstract
Background Residual malaria may be an important source for re-emerging of the malaria infection in the (pre)elimination era. Assessment of the residual-malaria in endemic areas as well as in (pre) elimination areas and identification of factors for residual malaria is needed to explore to support the evidence-based decision to the stakeholders and policymakers. MethodsThis study was conducted to explore the factors influencing the residual malaria infection among migrant workers in Myanmar by mixed-model method in two sentinel sites (one from pre-elimination areas and one from still endemic areas).ResultsA total of 102 migrant workers (65 in Bamauk and 37 in Shwegyin) were included in this study for quantitative assessment by using pretested questionnaires in household visits. Only 57.9% of the migrant in Shwegyin and 68.3% in Bmanuk were using the Long-lasting Insecticidal treated bed nets (LLINs) regularly although utilization of any bed net is high (91.0% in Shwegyin vs 79.9% in Bamauk). The mean of the number of the LLINs in their family was 2.89 (95%CI: 2.67-3.11) in Shwegyin and 1.64 (95%CI:1.48-1.81) in Bamauk. Unfortunately, their knowledge of the prevention of malaria was still low. Qualitative findings revealed that some of them reported no health information for malaria prevention was received in the last two years. ConclusionStrategy for distribution of LLINs for mobile/migrant populations should be reviewed and an appropriate action plan should be formulated. Health promotion activities for behavior change communication for malaria should be emphasized in the migrant population. Unlike the residence, an appropriate action plan should be considered for migrant groups, as some of the migrant groups are unable to be easily accessible by local health authorities nor village volunteers.
Severe Orthostatic Hypotension in Otherwise Uncomplicated Plasmodium Vivax Infection
Authors: Sivakorn Chaisith , Wilairatana Polrat , Krudsood Srivicha , Schultz Marcus J. , Techarang Tachpon , kheawsawaung Khanittha , Dondorp Arjen M. 
Published: November 2020

Abstract
Impaired autonomic control of postural homeostasis resulting in orthostatic hypotension has been described in falciparum malaria. However, severe orthostatic intolerance in Plasmodium vivax has not been previously reported. We describe a non-immune previously healthy Thai woman presenting with Plasmodium vivax infection with well-documented orthostatic hypotension. In addition to oral chloroquine and intravenous artesunate, the patient was treated with fluid resuscitation and norepinephrine. During hospitalization, her hemodynamic profile revealed orthostatic hypotension persisting for another three days after microscopic and polymerase chain reaction confirmed parasite clearance. Potential causes are discussed.
Ammonium Nitrate Regulated the Color Characteristic Changes of Pigments in Monascus Purpureus M9
Authors: Chen Di , Wang Yurong , Chen Mianhua , Fan Pei , Li Guiling , Wang Changlu 
Published: November 2020

Abstract
Monascus pigments (MPs) with different color characteristics, produced by submerged fermentation of Monascus purpureus M9, have potential application in food industry. In the present study, the effects and regulatory mechanisms of ammonium nitrate (AN) on the color characteristics of MPs were investigated. The concentration of intracellular pigments was significantly decreased when subjected to AN. The hue and lightness value indicated AN altered the total pigments appearance from original red to orange. The HPLC analysis for six major components of MPs showed that the production of rubropunctatin or monascorubrin, was significantly reduced to the undetectable level, whereas the the yields of monascin, ankaflavin, rubropunctamine and monascorubramine, were apparently increased with AN supplement. To be noted, via real-time quantitative PCR strategy, the expression level of mppG, closely relative to orange pigments biosynthesis, was significantly down-regulated. However, the expression of mppE, involved in yellow pigments pathway, was up-regulated. Moreover, the broth pH value was dropped to 2.5-3.5 in the fermentation process resulted from AN treatment, along with the increased extracellular polysaccharide biosynthesis. Taken together, the change of MPs categories and amounts by AN might be the driving force for the color characteristics variation in M. purpureus M9. The present study provided useful data for producing MPs with different compositions and modified color characteristics.
Characteristics of the Aspergillus oryzae GATA Transcription Factor Family and Expression Analysis under Temperature or Salt Stresses
Authors: Jiang Chunmiao , Lv Gongbo , He Bin , Zhang Zhe , Hu Zhihong , Zeng Bin 
Published: November 2020

Abstract
GATA transcription factors（TFs）are involved in the regulation of diverse growth processes and various environmental stimuli stresses. Although the analysis of GATA TFs involved in abiotic stress has been performed in plants and some fungi, information regarding GATA TFs in A. oryzae is extremely poor. Therefore, we identified seven GATA TFs from A. oryzae 3.042 genome and classified into six subgroups in NJ_tree, including a novel AoSnf5 with 20-residue between the Cys-X2-Cys motifs which was found in Aspergillus for the first time. Conserved motifs demonstrated that Aspergillus GATA TFs with similar motif compositions clustered into one subgroup, which suggests they might have similar genetic functions and further confirms the accuracy of the phylogenetic relationship. Moreover, the expression patterns of seven A. oryzae GATA TFs under temperature and salt stresses indicated that A. oryzae GATA TFs were mainly responsive to high-temperature and high salt stress. The PPI network of A. oryzae GATA TFs proposed some potentially interacting proteins. The comprehensive analysis of A. oryzae GATA TFs will be beneficial to understand their functional and evolutionary features and provide useful information for the further analyzing the role of GATA TFs in regulation of distinct environmental conditions in A. oryzae.
Cellular, molecular and biochemical impacts of silver nanoparticles on rat cerebellar cortex
Authors: Mohamed Eman , Kattaia Asmaa A.A. , Abdul-Maksoud Rehab , El-Baset Samia Abd 
Published: November 2020

Abstract
Background The excessive exposure to silver nanoparticles (Ag-NPs) has raised concerns about their possible risks to the human health. The brain is a highly vulnerable organ to nano-silver harmfulness. The aim of the current work was to evaluate the impacts of Ag-NPs exposure on the cerebellar cortex of rats especially changes at the ultrastructural and molecular levels. Methods Forty adult male albino rats were assigned to: control, vehicle control, Ag-NP-exposed groups (at doses of 10mg and 30mg/kg/day). Cerebellar cortex samples were processed for light and electron microscope examinations. Immunohistochemical localization of c-Jun N-terminal kinase (JNK), nuclear factor kappa beta (NF-κB) and Calbindin D28k (CB) proteins was performed. Tissue homogenates were prepared for analysis of gene expression of DNA damage inducible transcript 4 (Ddit4), flavin containing monooxygenase 2 (FMO2) and thioredoxin-interacting protein (Txnip). Serum levels of inflammatory cytokines interleukin-1 beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were also measured. Results Ag-NPs exposure resulted in activation of apoptosis cascades coupled with stimulation of oxidative stress and inflammation pathways. This was evident by the upregulation of Ddit4 gene expressions and JNK protein immune expressions. Alterations of redox homeostasis were verified by enhancement of Txnip and FMO2 gene expressions, favoring the activation of inflammatory responses by increasing NFκB immune expressions and serum inflammatory mediator levels. Another considerable cytotoxic effect was the reduction of CB immune expressions, the crucial regulator of intracellular calcium level. Conclusion Ag-NPs exposure provoked biochemical, cellular and molecular changes of rat cerebellar cortex in a dose-dependent manner.
Perceptions of Adolescent Pregnancy in Rural Contexts: An Approach based on Social Determination of health inequities
Authors: Palacios-Perdomo Herlan , Acosta-Ramirez Naydu 
Published: November 2020

Abstract
Background: The phenomenon of teen pregnancy as a social process in rural areas within settings of violence and peacebuilding are not well understood. The purpose of this study was to inquire into the perceptions of lived experiences in Colombian rural areas on the social forces and social determination of adolescent pregnancy. Methods: Qualitative study using semi-structured interviews and focus groups. Data triangulation included young people and other social actors from the Amaime river basin (municipalities of Palmira and El Cerrito in Colombia). Perceptions about the social conditions, specific ways of living, and lifestyles, were analyzed with approximations from phenomenology with the theoretical approach of social determination of health inequities. Results: Living conditions with strong social stigma and demand for social, political, and cultural opportunities were found. Regarding the way of living, an isolated young person is perceived with emotional gaps, sanctioned by the social group, and with little communication and information on sexual education. Regarding lifestyles, there are standardized youth behaviors that affect a person’s life plan and sexuality with precarious gender relations and with various types of violence that limit exercising peacebuilding. Conclusions: This study contributes to a pressing topic in sexual and reproductive health, with a novel approach that generates conclusions that contribute to comprehensively expanding the required interventions.
Production and Statistical Optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in Solid State Fermentation
Authors: El-Housseiny Ghadir S. , Ibrahim Asmaa A , Yassien Mahmoud A , Aboshanab Khaled 
Published: November 2020

Abstract
Background: Paromomycin is a 2 deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation.Results: Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. This value was higher than that obtained using submerged liquid fermentation using the same conditions. Optimization of environmental conditions using D optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion: Compared to submerged liquid fermentation, solid state fermentation resulted in higher paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, SSF is a superior alternative to SLF for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.
Standardizing and Optimizing Acupuncture Therapy for Irritable Bowel Syndrome: A Delphi Expert Consensus Study
Authors: Su Xin-Tong , Wang Li-Qiong , Zhang Na , Li Jin-Ling , Qi Ling-Yu , Wang Yu , Yang Jing-Wen , Shi Guang-Xia , Liu Cun-Zhi 
Published: November 2020

Abstract
Background: Acupuncture has been regarded as a potential physical approach for irritable bowel syndrome (IBS). However, there is still no adequate conclusive evidence on its efficacy and optimal therapeutic strategy of acupuncture for IBS. The purpose of this expert consensus study was to put forward some viable recommendations for clinical acupuncture practitioners in treating IBS.Methods: A panel composed of 74 acupuncturists, was convened to participate in a 2-round clinical issue investigation. Subsequently, systematic reviews on acupuncture for IBS were screened using 3 databases. An initial consensus voting list was generated from the results of clinical issue investigation and literature review. Finally, a multi-round Delphi vote was conducted to develop an expert consensus on these specific issues. 30 authoritative experts were required to respond with agreement, neutrality, or disagreement for the items on the consensus voting list. Consensus achievement was identified as the level of agreement on a given item > 80%.Results: Following a 2-round Delphi vote, there were 19 items achieving consensus; of which 5 items (26.32%) achieved complete consensus, and more than 80% expert agreement was reached for the other 14 items. These items can be roughly categorized into 3 major domains of acupuncture for IBS: (1) therapeutic effects, (2) therapeutic principles and protocols, (3) possible adverse events.Conclusions: Without any ready-made guidelines, this preliminary expert consensus could be helpful for acupuncturists in daily clinical practice and patients with IBS to acquire appropriate and standardized acupuncture treatment. In addition, it also points out the clinical issues which need to be further explored in future studies.
Lobectomy for lung cancer with a displaced left B1+2 and an anomalous pulmonary vein: a case report
Authors: Sakamoto Shinichi , Takizawa Hiromitsu , Kawakita Naoya , Tangoku Akira 
Published: November 2020

Abstract
Background: A displaced left B1+2 accompanied by an anomalous pulmonary vein is a rare condition involving complex structures. There is a risk of unexpected injuries to bronchi and blood vessels when patients with such anomalies undergo surgery for lung cancer.Case presentation: A 59-year-old male with suspected lung cancer in the left lower lobe was scheduled to undergo surgery. Chest computed tomography revealed a displaced B1+2 and hyperlobulation between S1+2 and S3, while the interlobar fissure between S1+2 and S6 was completely fused. Three-dimensional computed tomography (3D-CT) revealed an anomalous V1+2 joining the left inferior pulmonary vein and a branch of the V1+2 running between S1+2 and S6. We performed left lower lobectomy via video-assisted thoracic surgery, while taking care with the abovementioned anatomical structures. The strategy employed in this operation was to preserve V1+2 and confirm the locations of B1+2 and B6 when dividing the fissure.Conclusion: The aim of the surgical procedure performed in this case was to divide the fissure between S1+2 and the inferior lobe to reduce the risk of an unexpected bronchial injury. 3D-CT helps surgeons to understand the stereoscopic positional relationships among anatomical structures.
Predictions of daily contagions of CoViD-19 from October 10, 2020 until February 2021 in Italy with a new Epidemiologic FRActal Model plus Expolinear (EpiFRAME) to better frame the pandemic due to the 2B-BetaSarbeco-virus SARS-CoV-2
Authors: Rinzivillo Calogero , Conte Sergio , Casciaro Francesco , Bernardini Renato , Cascio Antonio , Benigni Elena , Monte Ines Paola , Kaleagasioglu Ferda , Wang Fang , Mordeniz Cengiz , Liberti Giuseppe , Spatola Corrado , Conte Elio 
Published: November 2020

Abstract
We study a new fractal model for prediction of contagions of CoViD-19 using experimental data from October 10, 2020 to predict contagions from November until February 2021 in Italy. We obtain that the time of the peak is estimated to be at November 11, 2020 when number of contagions will be about 38489 cases.
A comprehensive multivariate model of biopsychosocial factors associated with opioid misuse and use disorder in a 2017-2018 United States national survey
Authors: Ishino Francisco Alejandro Montiel , McNab Philip R. , Gilreath Tamika , Salmeron Bonita , Williams Faustine 
Published: November 2020

Abstract
Background: Few studies have comprehensively and contextually examined the relationship of variables associated with opioid use. Our purpose was to fill a critical gap in comprehensive risk models of opioid misuse and use disorder in the United States by identifying the most salient predictors.Methods: A multivariate logistic regression was used on the 2017 and 2018 National Survey on Drug Use and Health, which included all 50 states and the District of Columbia of the United States. The sample included all noninstitutionalized civilian adults aged 18 and older (N=85,580; weighted N=248,008,986). The outcome of opioid misuse and/or use disorder was based on reported prescription pain reliever and/or heroin use dependence, abuse, or misuse. Biopsychosocial predictors of opioid misuse and use disorder in addition to sociodemographic characteristics and other substance dependence or abuse were examined in our comprehensive model. Biopsychosocial characteristics included socioecological and health indicators. Criminality was the socioecological indicator. Health indicators included self-reported health, private health insurance, psychological distress, and suicidality. Sociodemographic variables included age, sex/gender, race/ethnicity, sexual identity, education, residence, income, and employment status. Substance dependence or abuse included both licit and illicit substances (i.e., nicotine, alcohol, marijuana, cocaine, inhalants, methamphetamine, tranquilizers, stimulants, sedatives). Results. The comprehensive model found that criminality (adjusted odds ratio [AOR]=2.58, 95% confidence interval [CI]=1.98-3.37, p<0.001), self-reported health (i.e., excellent compared to fair/poor [AOR=3.71, 95%CI=2.19-6.29, p<0.001], good [AOR=3.43, 95%CI=2.20-5.34, p<0.001], and very good [AOR=2.75, 95%CI=1.90-3.98, p<0.001]), no private health insurance (AOR=2.12, 95%CI=1.55-2.89, p<0.001), serious psychological distress (AOR=2.12, 95%CI=1.55-2.89, p<0.001), suicidality (AOR=1.58, 95%CI=1.17-2.14, p=0.004), and other substance dependence or abuse were significant predictors of opioid misuse and/or use disorder. Substances associated were nicotine (AOR=3.01, 95%CI=2.30-3.93, p<0.001), alcohol (AOR=1.40, 95%CI=1.02-1.92, p=0.038), marijuana (AOR=2.24, 95%CI=1.40-3.58, p=0.001), cocaine (AOR=3.92, 95%CI=2.14-7.17, p<0.001), methamphetamine (AOR=3.32, 95%CI=1.96-5.64, p<0.001), tranquilizers (AOR=16.72, 95%CI=9.75-28.65, p<0.001), and stimulants (AOR=2.45, 95%CI=1.03-5.87, p=0.044). Conclusions. Biopsychosocial characteristics such as socioecological and health indicators, as well as other substance dependence or abuse were stronger predictors of opioid misuse and use disorder than sociodemographic characteristics.
Improvement of the therapeutic capacity of insulin-producing cells trans-differentiated from human liver cells using engineered cell sheet&nbsp;
Authors: Lee Yu Na , Yi Hye-Jin , Seo Eun Hye , Oh Jooyun , Lee Song , Ferber Sarah , Okano Teruo , Shim Inkyong , Kim Song Cheol 
Published: November 2020

Abstract
Background: Although pancreatic islet transplantation therapy is ideal for diabetes patients, several hurdles have prevented it from becoming a standard treatment, including donor shortage and low engraftment efficacy. In this study, we prepared insulin-producing cells trans-differentiated from adult human liver cells as a new islet source.Also, cell sheets formation couldimprove differentiation efficiencyand graft survival. Methods: Liver cells were expanded in vitro and trans-differentiated to IPCs using adenovirus vectors carrying human genes for PDX1, NEUROD1 and MAFA. IPCs were seeded on temperature-responsive culture dishes to form cell sheets. Differentiation efficiency were confirmed by ß cell-specific gene expression, insulin production, and immunohistochemistry. IPCs suspension was injected by portal vein (PV), and IPCs sheet was transplanted on the liver surface of the diabetic nude mouse.The therapeutic effect of IPC sheet was evaluated by comparing blood glucose control, weight gain, histological evaluation and hepatotoxicity with IPCs injection group. Also, cell biodistribution was assessed by in vivo/ex vivo fluorescence image tagging.Results: Insulin gene expression and protein production were significantly increased on IPC sheets compared with those in IPCs cultured on conventional culture dishes. Transplanted IPC sheets displayed significantly higher engraftment efficiency and fewer transplanted cells in other organs than injected IPCs, and also lower liver toxicity, improved blood glucose levels, and weight gain. Immunohistochemical analyses of liver tissue revealed positive staining for PDX1 and insulin at 1, 2 and 4 weeks after IPCs transplantation.Conclusions: In conclusion, cell sheet formation enhanced the differentiation function and maturation of IPCsin vitro. Additionally, parameters for clinical application such as distribution, therapeutic efficacy, and toxicity were favorable. The cell sheet technique may be used with IPCs derived from various cell sources in clinical applications.