bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSXII-17 Trace minerals source influences the performance of Nellore cattle in the growing phase during the transition between dry to rainy season in Brazil
Authors: Cidrini Iorrano A , Lage Josiane F , Ferreira Igor , Oliveira Karla , Prados Laura F , Siqueira Gustavo R , Resende Flávio D 
Published: November 2020

Abstract
The relative bioavailability of trace minerals may vary between the sources and impact the animal performance. The study was carried out to evaluate the effect of TM source (Cu and Zn) on the liver concentration and performance of Nellore cattle supplemented in the growing phase during the transition between dry to rainy season. A hundred and twenty Nellore young bulls (BW = 349.72 ± 24 kg; 24 months), blocked by BW, were assigned randomly to twelve paddocks (six paddocks/treatment and 10 animals/paddock). Animals grazing Urochloa brizantha cv. Marandu and supplemented at 5 g/kg BW with soybean meal, corn and mineral mix (250 g/kg of CP and 650 g/kg de NDT, natural matter basis). Supplements consisted of 40 mg Cu and 148 mg Zn/kg DM from either inorganic TM (ITM) or hydroxy TM (HTM) Intellibond® sources. Liver samples were collected at the beginning and end of the study (three animals per paddock). Animals were weighed at each period (30 days) during three periods. Period was used as a REPEATED measurement for BW and values of initial BW as independent covariates for all variables. Paddock was considered experimental unit. Data were analyzed by ANOVA using PROC MIXED, SAS 9.4. The animals supplemented with HTM had higher (+0.037 kg/day) general ADG (day 0 to 90; P = 0.012), with 0.469 and 0.506 kg/day to ITM and HTM, respectively. Final BW (day 90) from animals fed HTM was 5.13 kg higher than ITM (391.97 vs. 397.11 kg BW; P = 0.030). No differences were found in the liver concentrations of Cu and Zn between ITM or HTM (P ≥ 0.288). Cu and Zn from HTM improve the performance of animals supplemented in pasture compared to ITM sources.
PSX-16 Genome-wide association studies for growth and carcass traits in Russian sheep
Authors: Deniskova Tatiana Evgenievna , Petrov Sergey N , Sermyagin Alexander A , Fornara Margaret S , Dotsev Arsen V , Reyer Henry , Wimmers Klaus , Bagirov Vugar A , Brem Gottfried , Zinovieva Natalia A 
Published: November 2020

Abstract
Breeding fast-growing sheep with good carcass traits is beneficial for lamb producers. However, genomic selection for relevant traits is still lagging in Russian sheep industry. In this regard, detection of the QTLs and candidate genes specific for local sheep is of great scientific and practical interest in Russia. In our research, we aimed to perform GWAS for growth and carcass traits in the QTL-mapping sheep population obtained from crossing slow- and fast-growing breeds. Backcrosses from (Katahdin× Romanov) × Romanov mapping population were genotyped with Ovine Infinium HD SNP BeadChip (Illumina, USA). Quality control in PLINK v.1.9 resulted in 459,868 SNPs which were used for GWAS. Threshold p-value for Bonferroni genome-wide significance was estimated as p = 1.09х10–7. The Manhattan plots were visualized in R package qqman. The measurements included body weight (BW), chest depth (CD), chest width (CW), and width at the iliac tubercle (WT) were recorded at the age of 6, 42 and 180 days. Significant SNPs including oar3_OAR7_44462236 (p = 8.40E-07), oar3_OAR7_44496608 (p = 8.40E-07) and oar3_OAR7_44975375 (p = 8.60E-07) were associated with WT at age of 180 days (13.31±0.23 cm). In addition, genomic regions located on chromosome 7 (44462236 – 44975375) and chromosome 2 (148067154 – 148077728) contained SNPs associated with CD at age of 180 days (21.42±0.39 cm) as well as with WT at age of 180 days. One SNPs (oar3_OAR4_4568047) on chromosome 4 and four SNPs on chromosome 7 (oar3_OAR7_44462236, oar3_OAR7_44496608, oar3_OAR7_44975375, and oar3_OAR7_45012432) were associated with BW at age of 180 days (22.61± 0.89 cm). The genes identified by GWAS were annotated. There were candidates responsible for bone metabolism, prenatal growth, skeletal muscle growth, related to meat quality and involved in carbohydrate and lipid metabolism. The reported study was funded by RFBR, project No. 17-29-08015.
PSX-23 Predicting feedstuff associative effects in goats
Authors: Tsukahara Yoko , Puchala Ryszard , Goetsch Arthur L 
Published: November 2020

Abstract
The objective was to develop means of predicting effects of supplementation on forage and total ME intake in goats. A database of treatment mean observations from the literature currently has 529 individual animal observations with ad libitum intake from 24 studies. Essential data were supplement and forage OM digestibility without supplementation or sufficient information for estimation, total diet OM digestibility, forage and supplement CP concentration and OM intake, and BW. Intake of ME was assumed 15.104 MJ/kg digested OM. For observations with supplementation, forage ME intake was the difference between total and supplement ME intake. The original database had 134 observations, although 24 were omitted because of questionable values and(or) unlikely estimates. Forage averaged 9.4 MJ/kg DM of ME (SD=1.77, 4.6–12.6), 59.1% OM digestibility without supplementation (SD=8.67, 42.9–77.1; AOMDIGFOR), and 8.1% CP (SD=3.72, 2.1–17.1) (PTCPFOR), and supplement was 12.3 MJ/kg ME (SD=1.38, 7.1–15.1) and 28.4% CP (SD=41.5, 0–288). Forage ME intake (MEIMBWFOR, kJ/kg BW0.75) was regressed against supplement ME intake (MEIMBWSUP, kJ/kg BW0.75), with inclusion of other variables tested. The equation explaining most variation (R2=0.77) was 155.9 ± 135.75 – (0.5403±0.04381 × MEIMBWSUP) – (41.0063±20.71841 × PTCPFOR) + (5.3497±2.31608 × AOMDIGFOR) + (0.74056±0.345002 × PTCPFOR × AOMDIGFOR), with P values of 0.253, &lt; 0.001, 0.050, 0.023, and 0.034, respectively. The mean difference between observed and predicted MEIMBWFOR (379±15.0 and 382±12.8, respectively) was -3±7.4, with minimum and maximum values of -181 and 167, respectively. The equation for the regression of residual MEIMBWFOR (observed – predicted) against predicted values was -10±22.3 + (0.018±0.0553 × predicted MEIMBWFOR), with the intercept and slope not different from 0 (P = 0.670 and 0.739, respectively). Equations describing associative effects can be used to predict the quantity of a particular supplement with a given forage necessary to meet nutrient requirements of goats in specific production settings.
PSX-7 Effect of varying levels of hempseed meal supplementation on animal performance, rumen fermentation, and blood metabolites of growing meat goats
Authors: Abrahamsen Frank W , Gurung Nar , Abdela Woubit , Reddy Gopal , Mullenix Kim 
Published: November 2020

Abstract
Hempseed meal (HSM) is a byproduct of hemp oil production and is high in crude protein, fiber, and fat, making it a potential feedstuff for ruminants. The objective of this study was to evaluate the effect of HSM supplementation on growth performance, rumen fermentation and blood chemistry profile of growing meat goats. Forty castrated, Boer cross goats were randomly assigned to one of the four treatments (n = 10): control, 10%, 20%, and 30% HSM supplementation. Data collected over a period of a 60-day feeding trial were analyzed utilizing the mixed model analysis function of SYSTAT, version 13. The result revealed total live weight gain decreased with the increasing levels of HSM supplementation 10.75, 9.53, 8.48, and 7.80 kg, for 0, 10, 20, and 30%, respectively. Average daily gain followed the same trend 0.179, 0.159, 0.141, and 0.13 kg, with a significant difference (P &lt; 0.05) observed between the control and 30 % supplementation. Conversely, feed to gain ratio increased with the increasing levels of supplementation 9.0, 10.2, 11.9, 12.2, likewise a significant difference was observed (P &lt; 0.05) between the control and 30% supplementation. Acetic, propionic, butyric, valeric, iso-valeric, and iso-butyric acid concentrations as well as the total VFA concentration decreased significantly (P &lt; 0.05) with the increasing level of supplementation. Acetic to propionic acid ratios increased with increasing level of supplementation 3.43, 4.36, 4.52, and 4.59, significant differences (P &lt; 0.05) were observed between control-20% and 30% HSM group. Serum glucose concentration decreased with an increasing rate of HSM supplementation while BUN concentration increased with no significant differences. These findings provide new insights into the feeding value of HSM for meat goats; however, further research needs to be conducted to determine the optimal level of supplementation.
PSX-2 Antioxidant system characteristics in Saanen goats depending on lactation period
Authors: Kurilova Anastasiia A , Bakhta Alesya A , Karpenko Larisa Y , Plemyashov Kirill , Kozitcyna Anna I , Balykina Anna B , Enukashvili Abram I , Polistovskaia Polina A , Ershova Olga N , Dushenina Olga A 
Published: November 2020

Abstract
Dairy goats require attention and high-quality feeding during the lactation due to metabolism intensity. The metabolic rate during different lactation periods in order to better understanding production physiology in Saanen goats is the case of interest. The aim of this research was to evaluate antioxidant system characteristics in Saanen goats depending on lactation period. The experiment was conducted in the north-western region of Russian Federation, in the laboratory of the biochemistry and physiology department, FSBEI of Higher Education «SPbSAVM». The experimental group included 30 Saanen goats, 2nd–3rd lactations, selected using matched pairs method. The blood samples were taken 3 times: at the start of the lactation (2 days after parturition), at peak lactation performance (45 days after parturition) and before the interlactation period (4 months after parturition). Control group included 30 non-lactating Saanen goats, same age. The blood samples in control group animal were taken once. The blood levels of lipid peroxygenation markers (malondialdehyde, dienketone and conjugated dienes) and superoxide dismutase and catalase activity were assessed by standard methods. Results presented as mean±standard error of the mean. Student’s t-test was used after proving normal distribution. Level of significance is labeled as P &lt; 0.05. The results are displayed in table 1 and 2. After analyzing the received data of antioxidant system characteristics the free radical oxidation highest intensity were revealed at the start of the lactation and at peak lactation performance, which indicates the development of oxidative stress in this periods. Also antioxidant enzymes’ activity was reduced during the peak lactation performance and remained at the control group level at the start of the lactation. This indicates free radical oxidation processes decompensation during the peak lactation performance. Thus, due to the development of decompensated oxidative stress in high milk producing ability animals during peak lactation, exogenous use of anti-oxidants is recommended.
PSIV-31 Do community-engaged learning courses benefit the equine student over a traditional laboratory setting?
Authors: Walters Avery , Cagle-Holtcamp Katie , Nicodemus Molly , Evans Holly , Lee Samantha , Gilmore Alicia 
Published: November 2020

Abstract
Vocational training is essential in developing both the skills and confidence to perform these activities within a profession. This type of training can be achieved through a traditional laboratory setting or within a laboratory centered around community engagement activities. While the benefits of both types of pedagogical strategies have been documented, comparison of these approaches to teaching in the equine environment is limited, and yet, development of skills and confidence is essential to the equine student as they step into the equine industry. Therefore, the objective of the study was to compare development of students’ confidence levels and skills through a traditional equine laboratory-setting to that of a community-engaged pedagogy. The study was conducted over the fall semester. A survey instrument was given at the beginning (pre-) and end (post-) of each semester for a weekly 2-hour traditional equine laboratory (TL; n = 36) and an equine course with a weekly 2-hour equine community engagement activity (CE; n = 47). Students in both course types completed a total of 30 hours handling horses during the semester. The survey consisted of 18 questions evaluating the student’s confidence in their perceived equine horsemanship skills. Students ranked their confidence from 1–5 with 5 indicating higher confidence. A skill assessment was completed by course instructors in conjunction with the students’ self-evaluation surveys. The skill assessment consisted of 10 questions with instructors ranking skills from 1–4 with 4 indicating higher skill level. Paired samples t-tests showed significant differences by the end of the semester in the skill assessment scores for both the TL and CE courses (P &lt; 0.01) and in the student-perceived confidence levels for the CE course (P &lt; 0.01). Although results indicate that both pedagogical strategies can be beneficial in equine skill development, community-engaged learning courses can provide a more effective approach to improving confidence in equine students.
PSI-30 Evaluation of urine estrone sulfate (P-Test) in beef cattle as an alternative pregnancy detection method
Authors: Hooten Bailey , Runyan Cheyenne , Roper David , Smith William B , Dias Nicholas W , Mercadante Vitor R G 
Published: November 2020

Abstract
Pregnancy detection in cattle can be expensive and labor intensive to producers. A simple, cost-efficient method of determining early pregnancy, chute side, is lacking within the industry. This study tested various proven pregnancy detection methods against an alternative method. Pregnancy was assessed by ultrasonography, blood serum pregnancy-specific protein B (PSPB), and urine estrone sulfate (“P-Test”). A fixed timed AI (FTAI) protocol was conducted on 14 predominantly British cattle housed at Tarleton State University in Stephenville, TX. Cows were synchronized using the co-synch plus 7 day CIDR protocol, with PGF injection of d 7. Heifers were synchronized similarly with the exception of GnRH injection on d 1. Estrotech patch scores (1 – 4, 1 showing little signs of estrus to 4 presenting signs of estrus) were utilized to indicate estrus activity prior to FTAI (21.4% vs. 78.6% females scored 1 vs. 4, respectively). FTAI was performed on protocol d 10 along with a 2mL injection of GnRH. Serum for PSPB analysis was collected at d 30 and 60 post insemination, while urine was sampled on d 60. Overall 53.8% (ultrasonography) and 71.4% (PSPB) of cattle were determined to be pregnant at d 30 post FTAI. Chi squared analysis proved that PSPB and ultrasound were similar at detecting pregnancy (P = 0.04). At d 60 “P-Test” color varied indicating differing levels of estrone sulfate. A t-test revealed increased PSPB from d 30–60 post FTAI (P &lt; 0.0001); however, “P-Test” could not predict similar results (P = 0.655). Data suggest the “P-Test” is not a consistent method to diagnose early pregnancy. Future studies may be warranted to investigate a more desirable time point for utilizing the chute side “P-Test” as a reliable method to determine pregnancy.
Development of a rule-based system to enhance the data consistency and usability of COBie datasheets☆
Authors: Kumar Vishal , Teo Ai Lin Evelyn 
Published: November 2020

Abstract
Building information modeling (BIM) for facilities management (FM) has been gaining considerable attention. Construction operations building information exchange (COBie) datasheets are conceptualized as an electronic format of data for FM handover extracted from the BIM model and supplemented with information from other sources. To build an efficient COBie datasheet, it is advocated to build and verify data at all stages of design and construction, commonly known as data drops. Nevertheless, data consistency verification is a difficult task pertaining to COBie's complex structure and data representation. This study aims to understand the challenges associated with the COBie datasheet verification and consistency checking process, especially during data drop stages, and develop a solution to mitigate these challenges. The study uses a combined methodology of design thinking and waterfall model from the software development process. The outcome of the research study manifests in a prototype application. The prototype application can help in verifying COBie datasheet consistency during data drop stages. Additionally, this study proposes a new dimension to utilize the COBie datasheet to track various asset-related changes in a project by comparing COBie datasheets and visualizing this data in a visually interactive manner using a property graph model.
Is salvage radiotherapy optimal to patients with occult cervical cancer undergoing inadvertent simple hysterectomy? A propensity score-matched analysis of a nationwide clinical oncology database
Authors: Wang Yanhong , Ouyang Yi , Su Jingjing , Xiao Lihua , Bai Zhigang , Cai Qunrong , Cao Xinping 
Published: November 2020

Abstract

Objective
We used National Cancer Institute’s Surveillance, Epidemiology and End Result database to assess the role of salvage radiotherapy for women with unanticipated cervical cancer after simple hysterectomy.

Methods
Patients with non-metastatic cervical cancer and meeting inclusion criteria were divided into three groups based on treatment strategy: simple hysterectomy, salvage radiotherapy after hysterectomy and radical surgery. Parallel propensity score-matched datasets were established for salvage radiotherapy group vs. simple hysterectomy group (matching ratio 1: 1), and salvage radiotherapy group vs. radical surgery group (matching ratio 1:2). The primary endpoint was the overall survival advantage of salvage radiotherapy over simple hysterectomy or radical surgery within the propensity score-matched datasets.

Results
In total, 2682 patients were recruited: 647 in the simple hysterectomy group, 564 in the salvage radiotherapy group and 1471 in the radical surgery group. Age, race, histology, grade, FIGO stage, insured and marital status and chemotherapy were comprised in propensity score-matched. Matching resulted in two comparison groups with neglectable differences in most variables, except for black race, FIGO stage III and chemotherapy in first matching. In the matched analysis for salvage radiotherapy vs. simple hysterectomy, the median follow-up time was 39 versus 32 months. In the matched analysis for salvage radiotherapy vs. radical surgery, the median follow-up time was 39 and 41 months, respectively. Salvage radiotherapy (HR 0.53, P = 0.046) significantly improved overall survival compared with simple hysterectomy, while salvage radiotherapy cannot achieve similar overall survival to radical surgery (HR 1.317, P = 0.045).

Conclusions
This is the largest study of the effect of salvage radiotherapy on overall survival in patients with unanticipated cervical cancer. Salvage radiotherapy can improve overall survival compared with hysterectomy alone, while cannot achieve comparable survival to radical surgery.
Protein phosphatase NtPP2C2b and MAP kinase NtMPK4 act in concert to modulate nicotine biosynthesis
Authors: Liu Xiaoyu , Singh Sanjay Kumar , Patra Barunava , Liu Yongliang , Wang Bingwu , Wang Jinsheng , Pattanaik Sitakanta , Yuan Ling 
Published: November 2020

Abstract
Protein phosphatases (PPs) and protein kinases (PKs) regulate numerous developmental, defense, and phytohormone signaling processes in plants. However, the underlying regulatory mechanism governing biosynthesis of specialized metabolites, such as alkaloids, by the combined effects of PPs and PKs, is insufficiently understood. Here, we report the characterization of a group B protein phosphatase type 2C, NtPP2C2b, that likely acts upstream of the NICOTINE2 locus APETALA 2/Ethylene Response Factors (AP2/ERFs), to regulate nicotine biosynthesis in tobacco. Similar to the nicotine pathway genes, NtPP2C2b is highly expressed in roots and induced by jasmonic acid (JA). Overexpression of NtPP2C2b in transgenic hairy roots or stable transgenic tobacco plants repressed nicotine pathway gene expression and reduced nicotine accumulation. Additionally, transient overexpression of NtPP2C2b, together with the NtERF221, repressed transactivation of the quinolinate phosphoribosyltransferase promoter in tobacco cells. We further demonstrate that the JA-responsive tobacco mitogen-activated protein kinase (MAPK) 4 interacts with NtPP2C2b in yeast and plant cells. Conditional overexpression of NtMPK4 in tobacco hairy roots up-regulated nicotine pathway gene expression and increased nicotine accumulation. Our findings suggest that a previously uncharacterized PP-PK module acts to modulate alkaloid biosynthesis, highlighting the importance of post-translational control in the biosynthesis of specialized plant metabolites.
Miniature inverted repeat transposable elements cis-regulate circular RNA expression and promote ethylene biosynthesis, reducing heat tolerance in Populus tomentosa
Authors: Song Yuepeng , Bu Chenhao , Chen Panfei , Liu Peng , Zhang Deqiang 
Published: November 2020

Abstract
Transposable elements (TEs) and their reverse complementary sequence pairs (RCPs) are enriched around loci that produce circular RNAs (circRNAs) in plants. However, the function of these TE–RCP pairs in modulating circRNA expression remains elusive. Here, we identified 4609 circRNAs in poplar (Populus tomentosa) and showed that miniature inverted repeat transposable elements (MITEs)–RCPs were enriched in circRNA flanking regions. Moreover, we used expression quantitative trait nucleotide (eQTN) mapping to decipher the cis-regulatory role of MITEs. eQTN results showed that 14 single-nucleotide polymorphisms (SNPs) were significantly associated with Circ_0000408 and Circ_0003418 levels and the lead associated SNPs were located in MITE–RCP regions, indicating that MITE–RCP sequence variations affect exon circularization. Overexpression and knockdown analysis showed that Circ_0003418 positively modulated its parental gene, which encodes the RING-type E3 ligase XBAT32, and specifically increased the expression of the PtoXBAT32.5 transcript variant, which lacks the E3 ubiquitin ligase domain. Under heat stress, PtoXBAT32.5 expression was induced with up-regulation of Circ_0003418, resulting in increased production of ethylene and peroxidation of membrane lipids. Our findings thus reveal the cis-regulatory mechanism by which a MITE–RCP pair affects circRNA abundance in poplar and indicate that Circ_0003418 is a negative regulator of poplar heat tolerance via the ubiquitin-mediated protein modification pathway.