bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ_0002232 Acts as a Potential Biomarker for AML and Reveals a Potential ceRNA Network of Circ_0002232/miR-92a-3p/PTEN
Authors: Deng Zhao-qun , Su Xiao-yu , Zhu Xin , Zhao Qian , Ke Jin-ming , Wu De-long , Yi Yun-yun , Yi Jing , Lin Jiang , Qian Jun 
Published: May 2020

Abstract
Background: PTEN, known as a classical tumor suppressor, has been reported to be down-expressed in acute myeloid leukemia (AML) and affected the progression of AML patients. Our research was aimed to investigate the expression level of circ_0002232, one of circular RNAs of PTEN, reveal the clinical significance and potential ceRNA interaction network in AML of it.Methods: Circ_0002232 expression in 117 AML patients and 48 controls was detected by using Real-time quantitative PCR.Results: Compared with controls, circ_0002232 was notably low-expressed in AML (P < 0.001). According to the result of receiver operating characteristic curve, circ_0002232 expression could distinguish AML patients from controls (P < 0.001). There were significant differences in patients’ age (P = 0.002), FAB classifications (P = 0.025), white blood cell count (P = 0.034) and platelet count (P = 0.047) between low-expressed circ_0002232 group and high-expressed circ_00022332 group. Moreover, there was a positive correlation between circ_0002232 expression and patients’ age (Pearson r = 0.256, P = 0.0053). Interestingly, we found that patients in low-expressed circ_0002232 group had better overall survival both in whole AML (P = 0.019) and non-APL AML (P = 0.044). Remarkably, the expression of circ_0002232 was positively correlated with PTEN (Pearson r = 0.769, P < 0.001). Furthermore, there was a negative correlation in AML between circ_0002232 and miR-92a-3p (Pearson r=-0.262, P = 0.032), miR-92a-3p and PTEN (Pearson r=-0.358, P = 0.019). Interaction prediction websites revealed that circ_0002232 might regulate the expression of PTEN through sponging miR-92a-3p and affect the process of AML.Conclusions: Circ_0002232, one of circRNAs of PTEN, was remarkably down-regulated in AML and could act as a promising biomarker for the diagnosis of AML. In addition, there might be a potential ceRNA interaction network of circ_0002232/miR-92a-3p/PTEN in AML.
Fraction exhaled nitric oxide and IOS as potential biomarkers for HDM immunotherapy in patients with allergic rhinitis and asthma
Authors: Zhou Jun , Du Wei , Xu Guofang , Wang Ping , Tao Yi , Sun Lin , Peng Bo , Dai Ranran , Tang Wei 
Published: May 2020

Abstract
Background：Allergen specific immunotherapy (AIT) is the only potential disease-modifying treatment of house dust mite (HDM) allergic asthma and rhinitis. The conventional treatment procedure lasted for 3 years, but biomarkers indicating early efficacy from this long-term treatment has not been well illustrated. In this single arm, observation study, we evaluated non-invasive airway inflammation parameters such as Exhaled nitric oxide (FeNO), nasal NO levels (FnNO), and small airway function parameter-impulse oscillometry system (IOS) as well as serum parameters Dermatophagoides pteronyssinus (Derp) specific IgG4 and sIgG4/IgE ratio as potential biomarkers for AIT.Methods：Patients with mild allergic asthma concomitant with allergic rhinitis were treated with subcutaneous immunotherapy of Der p extract. Clinical evaluations including symptom score and medication score, small airway function of spirometer and non-invasive airway inflammation parameters from the nose (FnNO) and lung (FeNO) were collected before and after half year and one year of AIT. Serum Derp specific IgE and IgG4 measurement data were also collected. Results：Symptoms and medication score of patients were alleviated both after half and one-year HDM immunotherapy according to VAS (visual analogue score) and medication score. Improved small airway function (evaluated through IOS) and reduced non-invasive airway allergic inflammation parameters both from the nose (FnNO) and lung (FeNO) were achieved after half and one year AIT. As to serum biomarkers, Der p specific IgG4 increased and Der P specific IgE/IgG4 decreased significantly after one year treatment.Conclusions: One year HDM subcutaneous allergen immunotherapy improved allergic symptoms and reduced medication score in patients with allergic asthma concomitant with rhinitis. Non-invasive airway inflammation parameters such as FeNO and FnNO and small airway function parameter IOS are potential biomarkers for evaluating early efficacy of AIT. Derp specific IgG4 as well as IgE/IgG4 ratio maybe good therapeutic predictors to reflect the efficacy of AIT in the first year of treatment.
Syngas production by chemical-looping gasification of waste activated carbon with iron-based oxygen carrier
Authors: Li Xiao , An Mei , Wu Man , Li Jinshuai , Zhang Xiuli , Wu Jilai , Yuan Sijie , Guo Qingjie 
Published: May 2020

Abstract
Waste activated carbon (WAC), as a typical solid waste, can be utilized by chemical looping gasification (CLG) technology with an iron-based oxygen carrier to produce valuable synthesis gas products. A series of experiments on WAC of the CLG process were carried out in a fixed-bed reactor. The operation parameters involving the OC/WAC mole ratio, steam flow rate and reaction temperature during WAC CLG reactions were investigated in detail. Further, the cyclic performance within 10 cycles was also discussed. Fresh and other representative oxygen carrier samples were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM) characterization methods. The results showed that the optimal OC/WAC mole ratio, steam flow rate and reaction temperature were determined to be 0.15, 0.10 mL/min, and 950 °C, respectively, to obtain high-quality syngas with relatively high carbon conversion. The iron-based oxygen carrier exhibited a stable cyclic performance during the multiple tests, following the reaction path of Fe2O3→Fe0.98O in the individual reduction process. Moreover, the iron-based oxygen carrier could be oxidized almost to its initial state after 10 redox tests, and no obvious sintering and agglomeration phenomena were observed. The WAC of CLG presents a new approach for the comprehensive utilization and disposal of solid waste, especially with low volatile feedstocks.
Efavirenz Based First Line Anti-Retroviral Drugs Disrupts Follicular Development-Evidence From Animal And Human Studies
Authors: Ohihoin Aigbe , Ohihoin Esther Ngozi , Iranloye Bolanle Olubusola , Olarenwaju Oladeji , Okafor Arinze , Oji Kalu , Kalejaiye Olufunto , Bakare Airat , Ujomu Ifeoma , Aina Olugbemiga , Ajibaye Shola , Okechukwu Ada , Kiladejo-Aderoba Adekemi , Medobi Frank , Ezechi Oliver , Taylor-Robinson Simon 
Published: May 2020

Abstract
INTRODUCTION: Interventions to eliminate mother to child transmission of HIV has led to very low vertical transmission rate, however there are genuine concerns on long term effect of anti-retroviral drugs on future reproductive functions of females. There is currently paucity of information regarding the effect of anti-retroviral agents such as Tenofovir, Efavirenz and Lamivudine on the ovarian reserve of patients receiving anti-retroviral drugs. METHODOLOGY: 1. ANIMAL STUDY: A total of 25 female Wistar rats, weighing between 150g to 170g were obtained and kept in the experimental animal house of the Nigerian Institute of Medical Research (NIMR) in Lagos, Nigeria. They were kept in clean cages at a temperature of 28 o C HUMAN STUDY: Women within the reproductive age range of 15-45 years were grouped into A, B and C categories. Group A was made up of HIV positive patients already on efavirenz based anti-retroviral therapy. Group B were HIV positive patients who were naïve to antiretroviral drugs at the time of recruitment. Group C participants were HIV negative women within the reproductive age range not being managed for Infertility. RESULTS: ANIMAL STUDY : The mean count of antral follicles was significantly lower in the group that received efavirenz (p=0.00). The corpus luteal count was significantly higher in the control group than study groups (p=0.033) HUMAN STUDY: Average value of FSH is significantly higher in the HIV positive group who had antiretroviral medications when compared to HIV negative group. (P=0.039).Antral follicular count was significantly reduced in the group that received anti retrovirals (ARV). P=0.009 CONCLUSION: Evidence from this animal and human study, tend to suggest that efavirenz based antiretroviral medication disrupts follicular development.
Network pharmacology-based mechanistic investigation of Jinshui Huanxian formula acing on idiopathic pulmonary fibrosis
Authors: Liu Tiantian , Xu Pengli , Qi Shuishui , Ke Shaorui , Hu Qin , Zhao Peng , Li Jiansheng 
Published: May 2020

Abstract
Background

Idiopathic pulmonary fibrosis (IPF) is a chronic respiratory disease with high incidence rate, morbidity and mortality. Jinshui Huanxian formula (JHF) is an empirical formula for the pathogenesis of lung-kidney qi deficiency and phlegm-blood stasis in pulmonary fibrosis. The purpose of this study is to explore the pharmacological mechanism of JHF action in IPF therapy by network interaction analysis.
Methods

The main active components and corresponding target genes of JHF were predicted using various databases. Two sets of IPF disease genes were obtained from the DisGeNET database and GEO database. Two sets of drug targets for IPF treatment were collected and the overlapping genes between disease genes and drug targets were analyzed. The target genes of JHF were intersected with the differentially expressed genes of IPF to obtain the predicted targets of JHF acting on IPF. The functions and pathways of predicted targets acting on IPF were analyzed by using DAVID and KEGG pathway database. Finally, the resulting drug target mechanisms were validated in a rat model of pulmonary fibrosis.
Results

494 active compounds and 1304 corresponding targets were screened. Intersection analysis showed that 4 genes were common genes of JHF targets, IPF disease genes and anti-IPF drugs in KEGG database, and these genes were targeted by several compounds of JHF respectively. 72 JHF targets were closely related with IPF, and were thus considered therapeutically relevant. The targets were screened and participated in the regulation of IPF through 18 pathways. The molecular functions of targets included regulation of oxidoreductase activity, kinase regulator activity, phosphotransferase activity and transmembrane receptor protein kinase activity. In vivo experiments showed that JHF could alleviate the degree of pulmonary fibrosis, including the decrease of collagen deposition and epithelial-mesenchymal transition.
Conclusions

This study explored the mechanisms of JHF from a systematic point of view, trying to identify the specific target pathways acing on IPF. Pharmacological network with in vivo validation explained the potential roles and mechanisms of JHF in IPF therapy.
SPINK7 Expression Changes Accompanied by HER2, P53 and RB1 may be Potential Biomarkers to Predict Oral Squamous Cell Carcinoma at Molecular Level
Authors: Penachiotti Gina , Valdez Fabio , González-Arriagada Wilfredo A , Montes Hector F , Parra Judith , Guida Valeria , Gómez Silvina E , Guerrero-Gimenez Martin E , Fernandez-Muñoz Juan M , Zoppino Felipe CM , Carón Rubén W , Ezquer Marcelo E , Fernández-Ramires Ricardo , Bruna Flavia A. 
Published: May 2020

Abstract
Background. The oral squamous cell carcinoma (OSCC) affects more than 300,000 patients annually worldwide with a high morbidity rate (37.8%). Several tumor biomarkers have been suggested to anticipate outcome but results were poor. Changes of SPINK7 and associated proteins in precancerous oral lesions could lead to genomic instability and promote oncogenesis. Our aim was to evaluate SPINK7as apotential molecular biomarkerpredictive of OSCC stages, compared with well-known molecules altered in cancer: HER2,TP53, RB1, NFKB and CYP4B1. Methods.Oral biopsies from patientswith dysplasia (n=33), less invasive(n=28) andhighly invasiveOSCC (n=18) were collected. 20 cases with a clinical suspicion but normal mucosa confirmedwere included ascontrol. Gene expression of SPINK7, P53,RB, NFKBand CYP4B1 were quantified by qPCR.SPINK7 levels were correlated with a cohort of 330 patients from the TCGA. Also,SPINK7, HER2, TP53, and RB1, were evaluated by immunohistofluorescence. One-way Kruskal-Wallis test and Dunn's post-hocwith a p<0.05 significance were used to data analyze.Results.In OSCC, SPINK7wasdown regulated andP53, RB, NFKB and CYP4B1were up regulatedrespect tothe others groups (p<0.001). Also,SPINK7 expressionwasdiminished in patients of TCGA(p=2.10e-6). In less invasive OSCC,SPINK7 and HER2 proteinswere decreasedandTP53 and RB1 significantly increasedrespect todysplasia and highly invasivegroups (p<0.05).Conclusion. Our results suggest that SPINK7changes accompanied of HER2, P53 and RB1 can be used to classify the molecular stage of epithelial oral lesion inthe OSCC, allowing a more accuratediagnosis to molecular and histopathological level.
An autonomous economical and efficient ureteroscopic irrigation system
Authors: Wang Tengteng , Chen Xiude , Xia Qinghua , Zhang Qi , Jin Xunbo 
Published: May 2020

Abstract
Background

In recent years, the minimally invasive technique for treating nephrolithiasis has been developed rapidly. Particularly, the flexible ureteroscopy has been extensively applied. For flexible ureteroscopy lithotripsy, the perfusion equipment is necessary to ensure a clear intraoperative field of view. This study was aimed to prepare a set of economical and efficient ureteroscopic irrigation system by some commonly used devices in clinical diagnosis and treatment practice.
Methods

In this study, according to requirements of the endoscopic surgery, the infusion apparatus, infusion apparatus extension tube, three-way plug valve, Luer one-way valve, ordinary syringe, and negative pressure spring were used to assemble the irrigation system with different functions.
Results

The autonomous perfusion device realized the real-time conversion of manual injection, continuous perfusion or negative pressure suction. The entire equipment has many advantages, including easily accessible raw materials, low cost, simple assembly process, easy operation, strong controllability, effective control ability for the intrarenal pressure, and high safety.
Conclusions

The commonly used medical devices were applied to assemble the autonomous ureteroscopic irrigation system, which is flexible, simple and cost-effective and thus can be applied in flexible ureteroscopic surgery.
Lathosterol in plasma measures cholesterol synthesis and identifies the efficiency of dietary phytosterols in reducing the plasma cholesterol concentration
Authors: Nunes Valeria Sutti , Ilha Angela Oliveira Godoy , Ferreira Guilherme Silva , Bombo Renata Paula Assis , Afonso Milessa Silva , Machado Roberta Marcondes , Nakandakare Edna Regina , Quintão Eder Carlos Rocha , Lottenberg Ana Maria Maria 
Published: May 2020

Abstract
Background

Because the plasma campesterol/cholesterol ratio does not differ between groups that absorb different amounts of cholesterol measured by the gold standard isotopic procedure we investigated whether the intestinal absorption of phytosterols (PS) depends on the body's cholesterol synthesis rate.
Methods

38 volunteers (58 ± 12 years; low-density lipoprotein cholesterol (LDL-C) ≥ 130 mg/dL) were randomly assigned to consume 400 mL/day of soy milk or soy milk + PS (1.6 g/day) for 4 weeks in a double-blind, placebo-controlled, cross-over study. Blood samples were collected and markers of phytosterol (PS) absorption and non-cholesterol sterol synthesis precursors measured.
Results

PS treatment reduced plasma total cholesterol concentration (-5,5%, p < 0.001), LDL-C (-7.6%, p < 0.001), triglycerides (-13.6%, p < 0.0085), and apolipoprotein B (apo B) (-6.3%, p < 0.008), without changing high density lipoprotein cholesterol (HDL-C concentration). The lathosterol-to-cholesterol ratio in serum predicted the serum cholesterol response to PS feeding where high basal cholesterol synthesis was associated with lack of response of plasma cholesterol to PS in the diet. Cholesterol synthesis being elevated in the placebo phase in non-responders to dietary PS indicated they were resistant to further synthesis rise, whereas responders, because they have lower synthesis rate than non-responders in the placebo phase, are capable expanding synthesis under the effect of alimentary PS.
Conclusions

responders absorbed more PS than non-responders likely resulting from responders delivering less endogenous cholesterol than non-responders into the intestinal lumen that facilitates greater absorption of PS by the intestine.
The Diagnostic and Prognostic Value of MiR-92a in Gastric Cancer
Authors: Guo Hanxu , Wang Yuhang , Li Yongtian , Wang Zishu 
Published: May 2020

Abstract
Background: miR-92a been proposed to have a significant role in the diagnosis andprognosis of different types of tumors, but the potential impact of its expression is still controversial due to the sample size. We conducted the meta-analysis to figure out whether miR-92a could be used as a detecting tool and prognosis of gastric cancer.Method: A literature search was performedby retrieving Web ofScience, PubMed, EMBASE, ChineseNational Knowledge Infrastructure (CNKI), VIP(Technology of Chongqing databases) and Wanfang databases (last update by February2020). The sensitivity (SEN), specificity (SPE), positive and negative likelihood ratios (PLR and NLR), diagnostic odds ratio (DOR), and area under the ROC curve (AUC) were pooled to explore the diagnostic performance of miR-92a.The pooled HRs and 95%CIs of miR-92a for overall survival (OS) were calculated to explore the prognostic performance of miR-92a. Subgroup and meta-analysis were further carried out to explore the heterogeneity.Results: 10 articles with12 studies were included. The pooled SEN and SPE were 0.76 (95% CI 0.64–0.85) and 0.79 (95% CI 0.63–0.90).Besides, the pooled PLR and NLR were3.7(95% CI 1.8–7.5)and 0.30 (95% CI 0.18–0.50), and the pooled DOR was 12(95% CI4–38). AUC was 0.84 (95% CI: 0.81–0.87), indicating asignificant value of miR-92a in the gastric cancer detection. For the prognostic analysis of miR-92a in gastric cancer, the univariate and multivariate data’s poor OS were 1.46(95% CI0.87-2.45) and 1.46(95% CI0.90-2.39).Conclusions: The present meta-analysis demonstrated that miR-92a could be a potential biomarker for thedetection of gastric cancer.Furthermore,high expression of miR-92a has a negative association with the survivalof patients, suggesting its potential as a prognostic indicator in gastric cancer.
Non-coplanar VMAT improves plan quality for locoregional radiotherapy of left-sided breast cancer with internal mammary nodes
Authors: Xu Yuan , Ma Pan , Hu Zhihu , Tian Yuan , Men Kuo , Wang Shulian , Xu Yingjie , Dai Jianrong 
Published: May 2020

Abstract
Purpose A planning study was performed to evaluate the feasibility of non-coplanar volume modulated arc therapy (ncVMAT) for locoregional radiotherapy of left-sided breast cancer with internal mammary nodes. Methods and materials Ten patients with left-sided breast cancer after breast conserving surgery were retrospectively studied. The planning target volumes (PTV) were contoured encompassing the whole breast/chestwall, internal mammary nodes (IMN) and supraclavicular nodal (SCN) region. For each patient, ncVMAT plan with 4 partial arcs composing of two coplanar arcs and two non-coplanar arcs with couch rotating to 90˚ was generated. The prescription dose was normalized to cover 95% of PTV with 50 Gy delivering in 25 fractions. For each ncVMAT plan, dosimetric parameters were compared with coplanar VMAT (coVMAT) plan optimizing with identical coplanar beam angle arrangement. Results With ncVMAT, the mean conformity index of the whole PTV increased from 0.82±0.02 to 0.86±0.01 (p=0.005), and there was no significant difference for the homogeneity index. The mean coverage of internal mammary target volume (PTVimn) with ncVMAT increased from 88.77±3.07% to 91.67±3.84% comparing with coVMAT (p=0.005). Meanwhile, the V55 in PTVscn and PTVimn decreased significantly from 5.76±6.03%, 13.73±7.77% to 2.94±3.93%, 3.75±3.36%, separately (p<0.01). For organs at risk, the average V30, V20, V10, V5 and Dmean of heart decreased from 5.13±3.22%, 12.18±7.29%, 33.98±15.73%, 72.03±16.79% and 10.47±2.97 Gy to 4.86±2.54%, 9.35±5.43%, 25.16±12.42%, 62.27±13.18% and 9.08±2.34 Gy, separately (p<0.01). Furthermore, ncVMAT significantly reduced the mean V20, V10, V5, Dmean of left lung, and V10, V5, Dmean of contralateral lung (p<0.01). A better sparing of LAD descending coronary artery and right breast were also shown with ncVMAT (p<0.01). Conclusions Compared to coVMAT, ncVMAT provides better conformity, lower V55 in PTVscn and PTVimn, better coverage of PTVimn, better dose sparing in heart, bilateral lung, LAD and right breast for locoregional radiotherapy of left-sided breast cancer with internal mammary nodes, which potentially increase the local control in IMN and reduce the risk of deleterious effects.
Quantification of Ovarian Granulosa Cells and Different Level Follicles in the Mouse Ovary Using Stereology Method, for Potential Application in 3D Bioengineered Ovary.
Authors: Zheng Jiahua , Zhang Jingkun , Tian Yanpeng , Song Yanbiao , Yang Zhengwei , Huang Xianghua 
Published: May 2020

Abstract
Background Stereology is an accurate method to obtain 3D quantitative information of microstructure based on the comprehensive observation of two-dimensional sections. Three-dimensional bioprinting of bioink to rebuild a bioengineered ovary has been proved to be a promising technique in preserving fertility. Fully understand the structure of the ovary, the distribution of the main cells and the numerical density of cells using stereology method is of great significance to the computer 3D reconstruction technology.Results The Cavalieri method gave the estimations that the ovary was (2.49 ± 0.32)mm3, the volume fraction of cortex, medulla, follicles and ovarian granulosa cells ( OGCs ) were 86.80%±2.82%, 13.20%±2.82%, 5.60%±0.25% and 81.19%±2.57%, respectively. From exact counts on serial sections, OGCs, primordial, preantral and antral follicles numerical density were estimated at ( 2.11 ± 0.28 ) × 106/mm3, 719.57 ± 18.04/mm3, 71.84 ± 3.93/mm3 and 17.29 ± 3.54/mm3, respectively. We estimated that there would be (2.11 ± 0.28) × 109 OGCs, (719.57 ± 18.04) × 103 primordial follicles, (71.84 ± 3.93) × 103 preantral follicles, (17.29 ± 3.54) × 103 antral follicles in one milliliter 3D bioink when 3D encapsulated mice ovarian constructs. The total number of the three stages follicles per ovary were 1791.74 ± 5.77, 178.88 ± 1.26, 43.04 ± 1.13, and the respective total number of OGCs and follicles per ovary were (5.26 ± 0.09) × 106, 2013.66 ± 8.16.Conclusions OGCs and follicles can be accurately measured using either the optical or physical disector. The application of these approaches for the study of 3D bioengineered ovary may contribute to a deeper understanding of the artificial ovary.