bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Influences of Palindromes in mRNA on Protein Folding Rates
Authors: Li Ruifang , Li Hong , Yang Sarula , Feng Xue 
Published: October 2019

Background:It is currently believed that protein folding rates are influenced by protein structure, environment and temperature, amino acid sequence and so on. We have been working for long to determine whether and in what ways mRNA affects the protein folding rate. A large number of palindromes aroused our attention in our previous research. Whether these palindromes do have important influences on protein folding rates and what’s the mechanism? Very few related studies are focused on these problems.Objective:In this article, our motivation is to find out if palindromes have important influences on protein folding rates and what’s the mechanism.Method:In this article, the parameters of the palindromes were defined and calculated, the linear regression analysis between the values of each parameter and the experimental protein folding rates were done. Furthermore, to compare the results of different kinds of proteins, proteins were classified into the two-state proteins and the multi-state proteins. For the two kinds of proteins, the above linear regression analysis were performed respectively.Results :Protein folding rates were negatively correlated to the palindrome frequencies for all proteins. An extremely significant negative linear correlation appeared in the relationship between palindrome densities and protein folding rates. And the repeatedly used bases by different palindromes simultaneously have an important effect on the relationship between palindrome density and protein folding rate.Conclusion:The palindromes have important influences on protein folding rates, and the repeatedly used bases in different palindromes simultaneously play a key role in influencing the protein folding rates.
Expression Levels of MicroRNA-125b in Serum Exosomes of Patients with Asthma of Different Severity and its Diagnostic Significance
Authors: Zhao Meizhen , Juanjuan Li , Weijia Fan , Jing Xie , Qiuhua Huang , Hua Zhang , Fuhe Liao , Hao Peng 
Published: October 2019

Background:
This study aimed to investigate the expression levels of microRNA (miRNA)-125b in serum
exosomes and its diagnostic efficacy for asthma severity.

Methods:
The study included 80 patients with untreated asthma and 80 healthy volunteers. The patients were divided
into 4 groups according to disease severity: 20 with the intermittent state, 20 with the mildly persistent state, 20 with
the moderately persistent state, and 20 with the severely persistent state. The expression levels of miRNA-125b in
serum exosomes of each group were detected using a quantitative polymerase chain reaction and compared. The
Spearman correlation analysis was used to study the correlation between the expression levels of miRNA-125b in
serum exosomes and asthma severity. The diagnostic efficacy of the expression levels of miRNA-125b in exosomes
for asthma severity was evaluated using the Receiver Operating Characteristic (ROC) curve.

Results:
The expression levels of miRNA-125b in serum exosomes of patients with intermittent, mildly persistent,
moderately persistent, and severely persistent asthma were all higher than those in the healthy control group, with
statistically significant differences. The expression levels of miRNA-125b were also statistically significantly different
among patients in each group. The Spearman correlation analysis showed a positive correlation of the relative
expression of miRNA-125b in serum exosomes with asthma severity. The area under the ROC curve of the diagnostic
efficacy of miRNA-125b in serum exosomes for patients with intermittent, mildly, moderately, and severely persistent
asthma was 0.7770, 0.8573, 0.9111, and 0.9995, respectively.

Conclusion:
The expression levels of miRNA-125b in serum exosomes had a high diagnostic efficacy and might
serve as a noninvasive diagnostic marker for asthma severity.
TPMT Genotype and Adverse Effects of Azathioprine among Jordanian Group
Authors: Mhanna Mohammed , Gharaibeh Munir Garaiba , Rashid Mohammad , Sharab Ahmad , Shehab Mohammad , Zihlif Malek 
Published: October 2019

Background:
Inflammatory Bowel Disease (IBD) is a common disease affecting many patients. This
disease is treated by azathioprine and TPMT genetic polymorphism affecting the patient’s tolerance. The aim of this
study is to investigate the importance of TMPT genotyping in reducing the incidence of adverse effects of azathioprine.

Methods:
One hundred and forty-one IBD patients were followed for azathioprine Adverse Drug Reaction (ADR).
Patients were genotyped for TPMT*2, TPMT*3A, TPMT*3B, TPMT*3C.

Results:
The frequency of Azathioprine adverse effect was about 35.5%. An association between TPMT genotypes
1/3A and 3B/3B and azathioprine related bone marrow suppression was found (P value ≤ 0.05).

Conclusion:
The findings suggest that there was a significant association between TPMT genotypes 1/3A and 3B/3B
and azathioprine related bone marrow suppression.
Monocarboxylate Transporter 1 in Brain Diseases and Cancers
Authors: Sun Yixin , Sun Jin , He Zhonggui , Wang Gang , Wang Yang , Zhao Dongyang , Wang Zhenjie , Luo Cong , Tian Chutong , Jiang Qikun 
Published: October 2019

Background:
Monocarboxylate Transporter 1 (MCT1), an important membrane transport protein, mediates
the translocation of monocarboxylates together with protons across biological membranes. Due to its pathological
significance, MCT1 plays an important role in the progression of some diseases, such as brain diseases and cancers.

Methods:
We summarize the general description of MCT1 and provide a comprehensive understanding of the role of
MCT1 in brain diseases and cancers. Furthermore, this review discusses the opportunities and challenges of MCT1-
targeting drug-delivery systems in the treatment of brain diseases and cancers.

Results:
In the brain, loss of MCT1 function is associated with pathologies of degeneration and injury of the nervous
system. In tumors, MCT1 regulates the activity of signaling pathways and controls the exchange of monocarboxylates
in aerobic glycolysis to affect tumor metabolism, proliferation and invasion. Meanwhile, MCT1 also acts as a
good biomarker for the prediction and diagnosis of cancer progressions.

Conclusion:
MCT1 is an attractive transporter in brain diseases and cancers. Moreover, the development of MCT1-
based small molecule drugs and MCT1 inhibitors in the clinic is promising. This review systematically summarizes
the basic characteristics of MCT1 and its role in brain diseases and cancers, laying the foundation for further research
on MCT1.
Design of Experiment, Preparation, and in vitro Biological Assessment of Human Amniotic Membrane Extract Loaded Nanoparticles
Authors: Shabani Avishan , Atyabi Fatemeh , Khoshayand Mohammad R. , Mahbod Reza , Cohan Reza A. , Akbarzadeh Iman , Bakhshandeh Haleh 
Published: October 2019

Background:
Human amniotic membrane grafting could be potentially useful in ocular surface
complications due to tissue similarity and the presence of factors that reduce inflammation, vascularization,
and scarring. However, considerations like donor-derived infectious risk and the requirement
of an invasive surgery limit the clinical application of such treatments. Moreover, the quick depletion
of bioactive factors after grafting reduces the efficacy of treatments. Therefore, in the current
study, the possibility of nano delivery of the bioactive factors extracted from the human amniotic
membrane to the ocular surface was investigated.

Materials and methods:
Nanoparticles were prepared using polyelectrolyte complexation from chitosan
and dextran sulfate. The effect of polymer ratio, pH, and the amount of extract on particle size
and encapsulation efficacy were studied using Box-Behnken response surface methodology.

Results:
The optimum condition was obtained as follows: 4.9:1 ratio of dextran sulfate to chitosan, 600
µL amniotic membrane extract, and pH of 6. The prepared nanoparticles had an average size of 213
nm with 77% encapsulation efficacy. In the release test, after 10 days, approximately 50% of entrapped
bioactive proteins were released from the nanocarriers in a controlled manner. Biological activity assessment
on endothelial cells revealed amniotic membrane extract loaded nanoparticles had a longer
and significant increase in anti-angiogenic effect when compared to the control.

Conclusion:
Our data elucidate the ability of nanotechnology in ocular targeted nano delivery of bioactive
compounds.
Human Dermal Fibroblast: A Promising Cellular Model to Study Biological Mechanisms of Major Depression and Antidepressant Drug Response
Authors: Mesdom Pierre , Colle Romain , Lebigot Elise , Trabado Séverine , Deflesselle Eric , Fève Bruno , Becquemont Laurent , Corruble Emmanuelle , Verstuyft Céline 
Published: October 2019

Background:Human dermal fibroblasts (HDF) can be used as a cellular model relatively easily and without genetic engineering. Therefore, HDF represent an interesting tool to study several human diseases including psychiatric disorders. Despite major depressive disorder (MDD) being the second cause of disability in the world, the efficacy of antidepressant drug (AD) treatment is not sufficient and the underlying mechanisms of MDD and the mechanisms of action of AD are poorly understood.Objective:The aim of this review is to highlight the potential of HDF in the study of cellular mechanisms involved in MDD pathophysiology and in the action of AD response.Methods:The first part is a systematic review following PRISMA guidelines on the use of HDF in MDD research. The second part reports the mechanisms and molecules both present in HDF and relevant regarding MDD pathophysiology and AD mechanisms of action.Results:HDFs from MDD patients have been investigated in a relatively small number of works and most of them focused on the adrenergic pathway and metabolism-related gene expression as compared to HDF from healthy controls. The second part listed an important number of papers demonstrating the presence of many molecular processes in HDF, involved in MDD and AD mechanisms of action.Conclusion:The imbalance in the number of papers between the two parts highlights the great and still underused potential of HDF, which stands out as a very promising tool in our understanding of MDD and AD mechanisms of action.
Solvent-mediated Highly Efficient Synthesis of [1,2,4]triazolo/benzimidazoloquinazolinone Derivatives
Authors: Malamiri Fatemeh , Khaksar Samad , Badri Rashid , Tahanpesar Elham 
Published: October 2019

Objective:
An efficient and catalyst-free procedure for the synthesis of [1,2,4]triazolo/benzimidazolo
quinazolinones has been developed in 2,2,2-trifluoroethanol or deep eutectic solvent(DESs) as a clean reaction
media.

Methods:
All of the obtained products are known compounds and identified by IR, 1HNMR,13CNMR, and
melting points.

Results:
Various products were obtained in good to excellent yields under reaction conditions.

Conclusion:
We have efficiently developed a practical and catalyst-free approach for the synthesis of
[1,2,4]triazolo/benzimidazolo quinazolinones employing TFE as a clean and reusable media.
Hyogo Framework for Action (HFA): A case study of Nigeria
Authors: Madu Christian N. , Ozumba Benjamin C. , Kuei Chuhua , Madu Ifeanyi E. , Nnadi Valentine E. , Ezeasor Ikenna C. , Eze Hillary I. , Odinkonigbo Uju , Ogbuene Emeka , Ewoh John , Mmom Prince C. 
Published: October 2019

Objective:
This paper uses the Analytic Hierarchy Process (AHP) to rank main
actions and their associated task areas outlined in the Hyogo Framework for Action (HFA)
in the case of Nigeria. The focus is on three major challenges namely (1) stakeholder inclusiveness,
(2) capacity building and communication and (3) local adaptation.

Methods:
The perceptions of a sample of 26 field disaster management experts on the HFA
were studied and analyzed using AHP. The study found that "Disaster Preparedness" is the
most important expected goal followed by "Risk Assessment and Early Warning."

Results:
Their priority indices are 0.258 and 0.219, respectively. "Local/City Governance"
however, shows poor performance with a priority index of 0.085. Monte Carlo simulation
was further applied to examine the robustness of the AHP assessments.

Conclusion:
The results are indicative of the perceptions of the performance levels attained
and the areas that need improvement.
Empleo del aprendizaje en la epidemiología clínica e impacto sobre metas académicas, científicas y sociales
Authors: Avilés-Ibarra Oswaldo José , Méndez-Domínguez Nina Isabel , Aguilar-Vargas Esteban 
Published: October 2019

Introducción: El abordaje epidemiológico permite a los profesionales del área de la salud identificar las tendencias poblacionales de morbilidad y problemas de salud, al mismo tiempo que ayuda a identificar grupos en riesgo y describir a las poblaciones en términos de sus perfiles de salud.
Objetivo: Describir el impacto del conocimiento situado sobre los resultados académicos, científicos y sociales de la materia de epidemiología clínica que se imparte en décimo semestre de la carrera de medicina de la Universidad Marista de Mérida.
Método: Estudio descriptivo, observacional, prospectivo en la asignatura de epidemiología clínica, se desarrolló un programa académico basado en la cognición situada que contempló el aprendizaje teórico al mismo tiempo que el aprendizaje práctico mediante el diseño, implementación, análisis y difusión de proyectos de salud de diseño epidemiológico, que adicionalmente permiten integrar los conocimientos clínico-prácticos y teóricos adquiridos durante la carrera.
Resultados: Los alumnos expresaron opiniones positivas acerca de la actividad de campo, lograron integrar los conocimientos teóricos, prácticos y clínicos de otras áreas de la medicina; reforzaron su vocación de servicio e interés por los problemas de salud de su comunidad, a la vez que expresaron interés por involucrarse en la continuación de sus proyectos al presentarlos en congresos, publicarlos y continuarlos en el desarrollo de sus temas de tesis.
Conclusiones: Este estudio permitió a los alumnos comprender el contexto cultural y social en el que se presentan los problemas de salud, y facilitar el análisis estadístico de los datos entendiendo su origen.
Hábitos de lectura en estudiantes de nuevo ingreso a la Licenciatura de Enfermería
Authors: Martínez Jaimes Patricia , Pérez Martínez Gabriel Alejandro , Pérez Martínez Manuel Gilberto 
Published: October 2019

Introducción: La lectura es un camino a la humanización y al desarrollo de sociedades e individuos. Diagnosticar el apego a la lectura, entre los estudiantes universitarios, es el primer paso para desarrollar estrategias que permitan su fomento.
Objetivo: Determinar hábitos de lectura en una población estudiantil.
Método: Se eligió mediante muestreo aleatorio simple, una muestra de estudiantes de primer semestre de Enfermería de la UVM Villahermosa, periodo 02-2018, se aplicó una encuesta sobre hábitos de lectura, se generó una base de datos en el software SPSS y se analizó para obtener resultados.
Resultados: Se analizó al 48% del universo, todos de la carrera de Enfermería y en su primer semestre universitario, 80.55% mujeres, edad promedio de 18.37 años. 46% vivía en hogares biparentales. 84% dependía de una beca económica para poder estudiar. Solo 9.37% refirió gusto por la lectura, 18.75% había leído 2 o más libros en el último mes. 84.37% solo leía libros de su carrera por obligación y dedicaban en promedio 1 hora a ello. 3.12% tenían padres que leían a diario y 34.37% de estos, eran profesionistas. 21.87% podía imaginar lo que leía, 81.24% refirió dificultad media a severa para leer, 68.75% presentaba dificultades para concentrarse y 34.37% comprendía lo que leía.
Conclusiones: La muestra fue primordialmente del sexo femenino, provino de hogares no biparentales, con problemas económicos, con padres no profesionistas y sin gusto por la lectura. La muestra no mostró hábitos adecuados de lectura, lo que afectaba su comprensión lectora, leían por obligación y no por placer. El pensamiento crítico de los estudiantes podría estar afectado y ello redundaría en su desempeño en el proceso de enseñanza-aprendizaje como estudiantes universitarios.
Multimedia educativa para el aprendizaje de la acupuntura y digitopuntura por estudiantes de Medicina
Authors: Lazo Herrera Luis Alberto , León Sánchez Bradly , Hernández-García Frank , Robaina-Castillo José Ignacio , Díaz Pita Gicela 
Published: October 2019

Introducción: La medicina natural y tradicional se aplica en Cuba como estrategia curricular en la Carrera de Medicina, constituyendo las tecnologías de la información y las comunicaciones uno de los métodos efectivos para su aplicación.
Objetivo: Confeccionar una multimedia educativa para el aprendizaje de la acupuntura y digitopuntura por estudiantes de Medicina.
Método: Se realizó un estudio de desarrollo tecnológico en la Universidad de Ciencias Médicas de Pinar del Río, de septiembre de 2017 a enero de 2018. Para la confección de la multimedia se identificaron como etapas de trabajo la búsqueda y recopilación de información, la selección de herramientas y el diseño. Se validó el producto mediante criterios aportados por 18 expertos en la materia. Se evaluó la efectividad de la utilización del producto mediante un experimento pedagógico a los 28 estudiantes que integraron la muestra.
Resultados: La multimedia muestra un diseño claro y atractivo. Ofrece una libre navegación donde se puede llegar, rápido y fácilmente al contenido deseado. En su totalidad, los expertos evaluaron el producto de “muy adecuado”, y todos los usuarios emitieron criterios de “bien” en los indicadores “Facilidad”, “Funcionalidad” y “Originalidad”, además de presentarse un comportamiento similar en los restantes. Al aplicar el Test de Mc- Nemar, todos los valores dieron significativos, con lo que se demostró la efectividad de la herramienta elaborada.
Conclusiones: La herramienta confeccionada permitió al estudiante de la Carrera de Medicina interactuar con los contenidos de la acupuntura y la digitopuntura, considerándose útil como material de apoyo en la docencia.
Pintar el ruido con silencio
Authors: Uribe Hanabergh Verónica 
Published: October 2019

Este artículo revisa el concepto del silencio en la representación visual del caso específico del Salto de Tequendama en Colombia. A partir del siglo XIX, la variable de lo sonoro se convirtió en un participante activo del acto pictórico y de la experiencia sublime del paisaje. El texto destaca que en las imágenes del Salto, el sonido estrepitoso se evidencia por medio de decisiones estéticas y formales que compensan el ruido y traen el silencio a la superficie como parte del acto contemplativo del observador de la obra de arte. Por medio de un breve recuento por la estética romántica del siglo XIX y por las descripciones literarias más significativas de los viajeros que enfatizaron en sus memorias el elemento del ruido de la cascada, así como el análisis visual de las principales representaciones de este accidente geográfico, este artículo propone una nueva mirada hacia estas imágenes canónicas en la memoria colectiva de la historia del arte colombiano.
ABORDAGEM ODONTOLÓGICA SOB ANESTESIA GERAL - SÍNDROME DE CORNÉLIA DE LANGE: RELATO DE CASO
Authors: Canadas Costa Maria Carolina 
Published: October 2019

RESUMO A Síndrome de Cornélia de Lange é uma desordem do desenvolvimento rara, diagnosticada principalmente pelas características faciais do portador, além de retardos mental e no crescimento. Portadores dessa síndrome são acometidos por doenças sistêmicas que podem agravar o controle de comportamento quando submetidos a atendimento médico/odontológico ou, inclusive, levar a óbito. A fim de se evitar que simples procedimentos sejam realizados em ambiente hospitalar, sob anestesia geral, devido à dependência de uma vida metódica e regrada, a assistência odontológica deve ser inserida precocemente na rotina dos portadores de tal síndrome. O objetivo deste trabalho é relatar o caso clínico do paciente V.M.S.D 12 anos de idade, sexo masculino, leucoderma, que se apresentou no Hospital Geral de Japuíba com odontalgia causada por cárie nos dentes 26 e 36, sendo submetido ao tratamento odontológico em ambiente hospitalar, sob anestesia geral; além de discutir os importantes cuidados necessários que o cirurgião – dentista deve ter para a realização de procedimentos odontológicos nesse paciente. Palavras-chave: Síndrome de Cornélia de Lange; Anestesia geral; Odontologia hospitalar ABSTRACT Corneal Lange Syndrome is a rare genetic disorder, diagnosed mainly by the facial features, in addition to growth and mental retardation. Patients with this syndrome are affected by systemic diseases that can worsen behavior control when undergoing medical / dental care or even lead to death. In order to avoid simple procedures being performed in a hospital environment under general anesthesia, due to the dependence on a methodical and regulated life, dental care should be inserted early in the routine of patients with such syndrome. This study aim to report the clinical case of V.M.S.D, 12year-old male patient, leucoderma, attended at Japuíba General Hospital presenting toothache caused by carie in permanent teeth 26 and 36, the procedure was performed in a hospital setting; in addition to discussing the important care that the dental surgeon must take to perform dental procedures in this patient. Keywords: Cornelia de Lange Syndrome; General anesthesia; Hospital dentistry
ENXERTO DO CORPO ADIPOSO BUCAL PARA FECHAMENTO DE FÍSTULA BUCO-SINUSAL: REVISÃO DE LITERATURA
Authors: Souza Cunha Farley 
Published: October 2019

RESUMOA Comunicação Buco-Sinusal (CBS) é um incidente cirúrgico que pode ocorrer durante a exodontia de dentes maxilares posteriores devido à intimidade de suas raízes com o assoalho do seio maxilar. As CBSs com diâmetro de até 2mm tendem a curar-se sem tratamento específico. Entretanto, as maiores que 3mm se não tratadas adequadamente, podem evoluir para complicações tais como sinusite maxilar recorrente, alteração do timbre nasal além da passagem de líquidos da cavidade bucal para a nasal através do óstio dentre outras. Este trabalho tem por objetivo discutir as técnicas mais utilizadas para o fechamento de CBS comparando-as com a técnica que utiliza o corpo adiposo bucal (Bola de Bichat), avaliando suas vantagens e desvantagens. As bases de dados digitais acessadas foram PubMed, MEDLINE, LILACS, Scopus compreendendo artigos em inglês entre o período de 1988 a 2016. Concluímos que o uso desta técnica apresenta excelentes resultados com poucas complicações, restituindo ao paciente uma condição de normalidade.Palavras-Chave: Fístula Bucoantral, Fístula Bucosinusal, Corpo adiposo bucal, bola de Bichat, Comunicação Bucosinusal. ABSTRACTOroantral Communication (OC) is a surgical incident that can occur during posterior maxillary teeth extraction due to the anatomical intimacy of its roots with the lining of the maxillary sinus. OC up to 2mm in diameter can cure without specific treatment. However, as larger than 3mm if left untreated, they may progress to complications such as recurrent maximal sinusitis, alteration of the nasal timbre beyond the passage of the oral cavity to a nasal through the ostium, among others. Several surgical techniques have been developed for the treatment of OC. This paper aims to review the literature on how to evaluate the most commonly used techniques for closing the OC, comparing them with a technique that uses the buccal adipose body (Bichat Ball), discussing its advantages and advantages. The digital databases accessed were PubMed, MEDLINE, LILACS and Scopus comprising articles in English from 1988 to 2016. This study allows us to conclude that the use of this technique presents excellent results with few complications, restoring the patient to a normal condition.Key-Words: Oroantral fistula, Oroantral communication, buccal fat pad, bichat fat pad