bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Integrated Methodology to Study Riparian Vegetation Dynamics: From Field Data to Impact Modeling
Authors: Latella M. , Bertagni M. B. , Vezza P. , Camporeale C. 
Published: July 2020

AbstractRiparian environments are highly dynamic ecosystems that support biodiversity and numerous services and that are conditioned by anthropogenic activities and climate change. In this work, we propose an integrated methodology that combines different research approaches—field studies and numerical and analytical modeling—in order to calibrate an ecohydrological stochastic model for riparian vegetation. The model yields vegetation biomass statistics and requires hydrological, topographical, and biological data as input. The biological parameters, namely, the carrying capacity and the flood‐related decay rate, are the target of the calibration as they are related to intrinsic features of vegetation and site‐specific environmental conditions. The calibration is here performed for two bars located within the riparian zone of the Cinca River (Spain). According to our results, the flood‐related decay rate has a spatial dependence that reflects the zonation of different plant species over the study site. The carrying capacity depends on the depth of the phreatic surface, and it is adequately described by a right‐skewed curve. The calibrated model well reproduces the actual biogeography of the Cinca riparian zone. The overall percentage absolute difference between the real and the computed biomass amounts to 9.3% and 3.3% for the two bars. The model is further used to predict the future evolution of riparian vegetation in a climate‐change scenario. The results show that the change of hydrological regime forecast by future climate projections may induce dramatic reduction of vegetation biomass and strongly modify the Cinca riparian biogeography.
Regulation of invasion and peritoneal dissemination of ovarian cancer by mesothelin manipulation
Authors: Coelho Ricardo , Ricardo Sara , Amaral Ana Luísa , Huang Yen-Lin , Nunes Mariana , Neves José Pedro , Mendes Nuno , López Mónica Nuñez , Bartosch Carla , Ferreira Verónica , Portugal Raquel , Lopes José Manuel , Almeida Raquel , Heinzelmann-Schwarz Viola , Jacob Francis , David Leonor 
Published: July 2020

AbstractPeritoneal dissemination is a particular form of metastasis typically observed in ovarian cancer and the major cause for poor patient’s outcome. Identification of the molecular players involved in ovarian cancer dissemination can offer an approach to develop treatment strategies to improve clinical prognosis. Here, we identified mesothelin (MSLN) as a crucial protein in the multistep process of peritoneal dissemination of ovarian cancer. We demonstrated that MSLN is overexpressed in primary and matched peritoneal metastasis of high-grade serous carcinomas (HGSC). Using several genetically engineered ovarian cancer cell lines, resulting in loss or gain of function, we found that MSLN increased cell survival in suspension and invasion of tumor cells through the mesothelial cell layer in vitro. Intraperitoneal xenografts established with MSLNhigh ovarian cancer cell lines showed enhanced tumor burden and spread within the peritoneal cavity. These findings provide strong evidences that MSLN is a key player in ovarian cancer progression by triggering peritoneal dissemination and provide support for further clinical investigation of MSLN as a therapeutic target in HGSC.
Expression of ETS1 in gastric epithelial cells positively regulate inflammatory response in Helicobacter pylori-associated gastritis
Authors: Teng Yongsheng , Cang Baocheng , Mao Fangyuan , Chen Weisan , Cheng Ping , Peng Liusheng , Luo Ping , Lu Dongshui , You Nan , Zou Quanming , Zhuang Yuan 
Published: July 2020

AbstractGastric epithelial cells (GECs) provide the first point of contact of the host by Helicobacter pylori (H. pylori), and the interaction between H. pylori and GECs plays a critical role in H. pylori-associated diseases. Aberrant expression of transcription factors (TFs) contributes to the pathogenesis of inflammatory disorders, including H. pylori-associated gastritis. ETS (E26 transformation specific) transcription factor family is one of the largest families of evolutionarily conserved TFs, regulating critical functions during cell homeostasis. We screened ETS family gene expression in H. pylori-infected mouse and human GECs and found that ETS1 (ETS proto-oncogene 1, transcription factor) expression was highly affected by H. pylori infection. Then, we reported that ETS1 was induced in GECs by H. pylori via cagA activated NF-κB pathway. Notably, we demonstrated that proinflammatory cytokines IL-1β and TNFα have synergistic effects on ETS1 expression during H. pylori infection in an NF-κB-pathway-dependent manner. RNA-seq assay and Gene-ontology functional analysis revealed that ETS1 positively regulate inflammatory response during H. pylori infection. Increased ETS1 is also detected in the gastric mucosa of mice and patients with H. pylori infection. Collectively, these data showed that ETS1 may play an important role in the pathogenesis of H. pylori-associated gastritis.
NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice
Authors: Léger Psylvia , Nachman Eliana , Richter Karsten , Tamietti Carole , Koch Jana , Burk Robin , Kummer Susann , Xin Qilin , Stanifer Megan , Bouloy Michèle , Boulant Steeve , Kräusslich Hans-Georg , Montagutelli Xavier , Flamand Marie , Nussbaum-Krammer Carmen , Lozach Pierre-Yves 
Published: July 2020

AbstractAmyloid fibrils result from the aggregation of host cell-encoded proteins, many giving rise to specific human illnesses such as Alzheimer’s disease. Here we show that the major virulence factor of Rift Valley fever virus, the protein NSs, forms filamentous structures in the brain of mice and affects mortality. NSs assembles into nuclear and cytosolic disulfide bond-dependent fibrillary aggregates in infected cells. NSs structural arrangements exhibit characteristics typical for amyloids, such as an ultrastructure of 12 nm-width fibrils, a strong detergent resistance, and interactions with the amyloid-binding dye Thioflavin-S. The assembly dynamics of viral amyloid-like fibrils can be visualized in real-time. They form spontaneously and grow in an amyloid fashion within 5 hours. Together, our results demonstrate that viruses can encode amyloid-like fibril-forming proteins and have strong implications for future research on amyloid aggregation and toxicity in general.
CD96, a new immune checkpoint, correlates with immune profile and clinical outcome of glioma
Authors: Liu Fangkun , Huang Jing , He Fengqiong , Ma Xiaodong , Fan Fan , Meng Ming , Zhuo Yang , Zhang Liyang 
Published: July 2020

AbstractCD96 is a promising candidate for immunotherapy. However, its role and importance in glioma remains unknown. We thus aimed to genetically and clinically characterize CD96 expression in gliomas. For this, we extracted RNA-seq data of 699 glioma samples from the TCGA dataset and validated these findings using the CGGA dataset comprising 325 glioma samples. Clinical and isocitrate dehydrogenase (IDH) mutation status were also analyzed. Various packages in R language were mainly used for statistical analysis. CD96 expression was significantly up-regulated in high-grade, IDH-wildtype, and mesenchymal-molecular subtype gliomas based on TCGA data, which was validated using the CGGA dataset. Subsequent gene ontology analysis of both datasets suggested that genes relevant to CD96 are mainly involved in immune functions in glioma as such genes were positively correlated with CD96 expression. To further explore the relationship between CD96 and immune responses, we selected seven immune-related metagenes and found that CD96 expression was positively correlated with HCK, LCK, and MHC II in the CGGA and TCGA cohorts but negatively associated with IgG. Further, Pearson correlation analysis showed that CD96 is associated with TIGIT, CD226, CRTAM, TIM-3, PD-L1, CTLA-4, and STAT3, indicating the additive antitumoral effects of these checkpoint proteins. CD96 was also suggested to play an important role in immune responses and positively collaborate with other checkpoint members. These findings show that CD96 is promising candidate for immunotherapy, and that such agents could complement current immunotherapy strategies for glioma.
Impact of hearing aid noise reduction algorithms on the speech-evoked auditory brainstem response
Authors: Seol Hye Yoon , Park Suyeon , Ji Yoon Sang , Hong Sung Hwa , Moon Il Joon 
Published: July 2020

AbstractThe purposes of this study are to investigate the neural representation of a speech stimulus in the auditory system of individuals with normal hearing (NH) and those with hearing aids (HAs) and to explore the impact of noise reduction algorithms (NR) on auditory brainstem response to complex sounds (cABR). Twenty NH individuals and 28 HA users completed puretone audiometry, the Korean version of the Hearing in Noise Test (K-HINT), and cABR. In 0 and +5 dB signal-to-noise ratios (SNRs), the NH group was tested in /da/ only (quiet) and /da/ with white noise (WN) conditions while the HA group was tested in /da/ only, /da/ WN, /da/ WN NR ON, and /da/ WN NR OFF conditions. Significant differences were observed between /da/ only and /da/ WN conditions for F0 in both groups, but no SNR effect was observed for both groups. Findings of this study are consistent with previous literature that diminished cABR amplitudes indicate reduced representation of sounds in the auditory system. This is the first to examine the effect of a specific HA feature on cABR responses.
Low geomagnetic field strength during End-Cretaceous Deccan volcanism and whole mantle convection
Authors: T. Radhakrishna , Mohamed Asanulla R. , M. Venkateshwarlu , S. Soumya G. 
Published: July 2020

AbstractKnowledge about long-term variation of the geomagnetic dipole field remains in its nascent stage because of the paucity of reliable experimental data over geological periods. Here, we present the first robust experimental data from the largest Cretaceous flood basalt province on Earth, the ~65–66 Ma Deccan basalt within a thick (1250 m) unbiased stratigraphic section down to the basement, recovered from a drill hole of the Koyna Deep Scientific Drilling Project in the Western Ghats, India. Critical analysis of the result along with similar results of the Cretaceous age find that (i) the dipole moment during the end Cretaceous Deccan eruption is the lowest in whole of Cretaceous (ii) dipole moment at the onset/termination of the Cretaceous Normal Superchron is apparently lower relative to that in mid-superchron, however, such differences cannot be deciphered in shorter polarities probably because of insufficient time to develop recognizable variations (iii) inverse relation between dipole moment and reversal rate is lacking and (iv) a cause and effect relation between core-mantle boundary heat flux and low dipole moment that appears to be the principle governing factor in forming the Large Igneous Provinces on the surface of earth.
An ultra-high bandwidth nano-electronic interface to the interior of living cells with integrated fluorescence readout of metabolic activity
Authors: Ren Dandan , Nemati Zahra , Lee Chia-Hung , Li Jinfeng , Haddadi Kamel , Wallace Douglas C. , Burke Peter J. 
Published: July 2020

AbstractWe present the first ever broadband, calibrated electrical connection to the inside of a cell. The interior of a vital, living cell contains multiple dynamic and electrically active organelles such as mitochondria, chloroplasts, lysosomes, and the endoplasmic reticulum. However, little is known about the detailed electrical activity inside the cell. Here we show an ultra-high bandwidth nano-electronic interface to the interior of living cells with integrated fluorescence readout of metabolic activity. On-chip/on-petri dish nanoscale capacitance calibration standards are used to quantify the electronic coupling from bench to cell from DC to 26 GHz (with cell images at 22 GHz). The interaction of static to high frequency electromagnetic fields with the cell constituents induce currents of free charges and local reorganization of linked charges. As such, this enables a direct, calibrated, quantitative, nanoscale electronic interface to the interior of living cells. The interface could have a variety of applications in interfacing life sciences to nano-electronics, including electronic assays of membrane potential dynamics, nano-electronic actuation of cellular activity, and tomographic, nano-radar imaging of the morphology of vital organelles in the cytoplasm, during all phases of the cell life cycle (from development to senescence), under a variety of physiological environments, and under a broad suite of pharmacological manipulations.
Analysis of the StoRM cohort reveals physical activity to be associated with survival in metastatic breast cancer
Authors: Delrieu Lidia , Jacquet Emmanuelle , Segura-Ferlay Céline , Blanc Ellen , Febvey-Combes Olivia , Friedenreich Christine , Romieu Gilles , Jacot William , Rios Maria , Heudel Pierre-Etienne , Roemer-Becuwe Célia , Jouannaud Christelle , Tredan Olivier , Chaigneau Loïc , Arnedos Monica , Orfeuvre Hubert , Quenel-Tueux Nathalie , Jacquin Jean-Philippe , Ferrero Jean-Marc , Moullet Isabelle , Abadie-Lacourtoisie Sophie , Penault-Llorca Frédérique , Cox David , Bachelot Thomas 
Published: July 2020

AbstractBenefits of physical activity are widely demonstrated for early stage cancers but few studies have focused on metastatic disease. The purpose of this study was to determine the impact of physical activity on survival in patients with metastatic breast cancer. We conducted a secondary analysis of the national, multicentric, non-randomized, prospective cohort SNPs to Risk of Metastasis (StoRM) study. The level of physical activity was self-reported at inclusion and divided into three categories of physical activity: light level, moderate level, and vigorous level. Overall, 833 patients (56.2%) completed the physical activity questionnaire at baseline on average physical activity during the previous year: 11.6% had a light level of physical activity, 69.0% achieved moderate levels of physical activity and 19.3% reported vigorous levels of physical activity. After adjustment for confounding, physical activity was not statistically significantly associated with overall survival in the whole population. Subgroup analysis identified that both vigorous and moderate physical activity were associated with statistically significantly improved overall survival compared to light physical activity level only in the HER2 positive subgroup (HR 0.23; 95% CI 0.07–0.70, p = 0.01 and HR 0.38; 95% CI 0.15–0.96, p = 0.04). Physical activity done during the previous year was associated with survival in HER2 positive metastatic breast cancer patients. These results suggest that overall survival in metastatic breast cancer patients could be improved through physical activity which should be considered as a complementary intervention for these individuals. The study showed that moderate/vigorous levels of physical activity were associated with better overall survival, and that these associations remained statistically significant in multivariate analysis in the HER2 positive subgroup. These results have clinical relevance and justify the recommendations for physical activity interventions in metastatic breast cancer.
Late mortality in survivors of childhood cancer in Hungary
Authors: Jakab Zsuzsanna , Garami Miklos , Bartyik Katalin , Csoka Monika , Erdelyi Daniel Janos , Hauser Peter , Juhasz Attila , Kelemen Agnes , Krivan Gergely , Masat Peter , Müller Judit , Nagy Csilla , Peter György , Renyi Imre , Szegedi Istvan , Vojcek Agnes , Zombori Marianna , Bardi Edit , Kovacs Gabor 
Published: July 2020

AbstractThe Hungarian Pediatric Oncology Network provides centralized treatment and population-based registration for cases of childhood cancer since 1973. We collected and analized data on late mortality, secondary malignancies and cardiac diseases in survivors (> 5 years) of childhood cancer to evaluate long-term risks. We extracted all solid tumour cases (3,650 followed up for 5–39.3 years, diagnosis: 1973–2008) from the database of the Hungarian Childhood Cancer Registry and checked against the Population Registry. Among the 301 patients who died after 5 years (8.2%) the most common causes of death were progression of primary cancer (52.5%), secondary malignancies (16%) and cardiovascular diseases (8%). Late mortality rates (SMR, total: 35,006 pyrs) showed highly elevated risk of death (SMR: 10.7 95% CI 9–12.4) for the second 5 years of follow up and moderately elevated risk for 10-year survivors (SMR: 3.5 95% CI 3–4.1). Marked differences were detected in the pattern of causes of death between diagnostic groups of primary cancer; with highest risks beyond 10 years for CNS tumours, Hodgkin disease, osteosarcoma and advanced stage neuroblastoma. The longstanding mortality risk for 5-year survivors underlines the need for tailored long-term follow-up and monitoring of late consequences according to the context of different primary diseases of childhood cancer.
Optimization of spin Hall magnetoresistance in heavy-metal/ferromagnetic-metal bilayers
Authors: Karwacki Łukasz , Grochot Krzysztof , Łazarski Stanisław , Skowroński Witold , Kanak Jarosław , Powroźnik Wiesław , Barnaś Józef , Stobiecki Feliks , Stobiecki Tomasz 
Published: July 2020

AbstractWe present experimental data and their theoretical description on spin Hall magnetoresistance (SMR) in bilayers consisting of a heavy metal (H) coupled to in-plane magnetized ferromagnetic metal (F), and determine contributions to the magnetoresistance due to SMR and anisotropic magnetoresistance (AMR) in five different bilayer systems: $$\hbox {W}/\text {Co}_{20}\text {Fe}_{60}\text {B}_{20}$$W/Co20Fe60B20, $$\text {Co}_{20}\text {Fe}_{60}\text {B}_{20}/\hbox {Pt}$$Co20Fe60B20/Pt, $$\hbox {Au}/\text {Co}_{20}\text {Fe}_{60}\text {B}_{20}$$Au/Co20Fe60B20, W/Co, and Co/Pt. The devices used for experiments have different interfacial properties due to either amorphous or crystalline structures of constitutent layers. To determine magnetoresistance contributions and to allow for optimization, the AMR is explicitly included in the diffusion transport equations in the ferromagnets. The results allow determination of different contributions to the magnetoresistance, which can play an important role in optimizing prospective magnetic stray field sensors. They also may be useful in the determination of spin transport properties of metallic magnetic heterostructures in other experiments based on magnetoresistance measurements.
Glycated albumin precipitation using aptamer conjugated magnetic nanoparticles
Authors: Fayazi R. , Habibi-Rezaei M. , Heiat M. , Javadi-Zarnaghi F. , Taheri R. A. 
Published: July 2020

AbstractTo develop a strategy for the elimination of prefibrillar amyloid aggregates, a three-step non-modified DNA aptamer conjugation on silica-coated magnetic nanoparticles was carried out to achieve aptamer conjugated on MNP (Ap-SiMNP). Prefibrillar amyloid aggregates are generated under a diabetic condition which are prominently participated in developing diabetic complications. The binding properties of candidate DNA aptamer against serum albumin prefibrillar amyloid aggregates (AA20) were verified using electrophoretic mobility shift assay (EMSA) and surface plasmon resonance spectroscopy (SPR) analysis. The chloro-functionalized silica-coated MNPs were synthesized then a nano-targeting structure as aptamer conjugated on MNP (Ap-SiMNP) was constructed. Finally, Ap-SiMNP was verified for specific binding efficiency and AA20 removal using an external magnetic field. The candidate aptamer showed a high binding capacity at EMSA and SPR analysis (KD = 3.4 × 10─9 M) and successfully used to construct Ap-SiMNP. Here, we show a proof of concept for an efficient bio-scavenger asAp-SiMNP to provide a promising opportunity to consider as a possible strategy to overcome some diabetic complications through specific binding/removal of toxic AA20 species.
Analysis of non-conjugated steroids in water using paper spray mass spectrometry
Authors: Jjunju Fred. P. M. , Damon Deidre E. , Romero-Perez David , Young Iain S. , Ward Ryan J. , Marshall Alan , Maher Simon , Badu-Tawiah Abraham K. 
Published: July 2020

AbstractA novel strategy for the direct analysis of non-conjugated steroids in water using paper spray mass spectrometry (PS-MS) has been developed. PS-MS was used in the identification and quantification of non-conjugated (free) steroids in fish tank water samples. Data shown herein indicates that individual amounts of free steroids can be detected in aqua as low as; 0.17 ng/µL, 0.039 ng/µL, 0.43 ng/µL, 0.0076 ng/µL for aldosterone, corticosterone, cortisol, and β-estrone, respectively, and with an average relative standard deviation of ca. < 10% in the positive ion mode using PS-MS/MS. Direct detection of free steroids in a raw water mixture, from aquaculture, without prior sample preparation is demonstrated. The presence of free steroids released in fish water samples was confirmed via tandem mass spectrometry using collision-induced dissociation. This approach shows promise for rapid and direct water quality monitoring to provide a holistic assessment of non-conjugated steroids in aqua.
Influencing electroclinical features and prognostic factors in patients with anti-NMDAR encephalitis: a cohort follow-up study in Chinese patients
Authors: Wang Yingxin , Miao Ailiang , Shi Yongwei , Ge Jianqing , Wang Lingling , Yu Chuanyong , Xu Haiyan , Yu Yuanwen , Huang Shuyang , Li Yihan , Wang Xiaoshan 
Published: July 2020

AbstractThe clinical manifestations of patients with anti-N-methyl-d-aspartate receptor (anti-NMDAR) encephalitis in East China and factors associated with prognosis were analyzed. A retrospective study of 106 patients (58 females; 48 males) with anti-NMDAR encephalitis in East China was carried out from June 2015 to February 2019. Clinical features and factors influencing outcomes were reviewed. Behavioral changes were observed in 74.5% (79/106) of patients, and comprised the initial symptoms in 61.3% (65/106). Seizures were observed in 67% (71/106) of patients, and served as initial symptoms in 31.1% (33/106). A total of 54.9% (39/71) of seizures were focal seizures. More clinical symptoms were observed in female patients than in male patients (P = 0.000). Similarly, background activity (BA) with high cerebrospinal fluid (CSF) antibody titers at the peak stage was more severe in female patients than in male patients (P = 0.000). The Binary logistic regression and receiver operating characteristic (ROC) curve analyses revealed the factors associated with poor outcomes included consciousness disturbance (OR 4.907, 95% CI 1.653–14.562, P = 0.004; area: 65.4%, sensitivity: 44.2%, specificity: 86.5%, P = 0.014), EEG BA (OR 3.743, 95% CI 1.766–7.932, P = 0.001; area: 76.6%, sensitivity: 73%, specificity: 75%, P = 0.000), number of symptoms (OR 2.911, 95% CI 1.811–4.679, P = 0.000; area: 77.1%, sensitivity: 59.5%, specificity: 78.6%, P = 0.000) and CSF antibody titer (OR 31.778, 95% CI 8.891–113.57, P = 0.000; area: 83.9%, sensitivity: 89.2%, specificity: 78.6%, P = 0.000). EEG BA and number of symptoms were associated with CSF antibody titers. Consciousness disturbances, EEG BA, number of symptoms and CSF antibody titers served as predictors of poor outcomes.