bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human papillomavirus and human telomerase RNA component gene in cervical cancer progression
Authors: Liu Yang , Fan Pianping , Yang Yingying , Xu Changjun , Huang Yajuan , Li Daizhu , Qing Qing , Sun Chunyi , Zhou Honglin 
Published: November 2019

AbstractThis study aimed to examine hTERC gene in different grades of cervical intraepithelial neoplasia (CIN) and cervical cancer, and the association between hTERC and high risk-human papillomavirus (HR-HPV) infection. Patients who underwent cervical cancer screening at the Second Affiliated Hospital of Kunming Medical University between October 2010 and December 2011 were enrolled. All patients underwent liquid-based cytology test and hybrid capture 2 (HC2) for HPV detection. hTERC was examined using fluorescence in situ hybridization (FISH). Cervical colposcopy biopsy was performed if any of the three results was positive. HC2, FISH, and pathology were compared. A total of 1200 women underwent screening, 150 patients underwent cervical biopsy: 32 in the normal group, 38 in the CIN1 group, 66 in the CIN2/3 group, and 14 in the invasive cervical cancer group. More patients had HR-HPV infection in the CIN2/3 group and ICC group compared with the CIN1 group. hTERC increased with increasing histological dysplasia. There was significant difference in hTERC positive rate between each of the three groups. More patients with hTERC gene amplification were observed in the positive HR-HPV group than in the HR-HPV negative group. In conclusion, hTERC is a potential marker for precancerous cervical cancer lesions. hTERC might be correlated with HR-HPV infection in cervical diseases.
Examining environmental contaminant mixtures among adults with type 2 diabetes in the Cree First Nation communities of Eeyou Istchee, Canada
Authors: Zuk Aleksandra M. , Tsuji Leonard J. S. , Nieboer Evert , Martin Ian D. , Liberda Eric N. 
Published: November 2019

AbstractType 2 diabetes mellitus (T2DM) disproportionately affects Indigenous populations. It is possible that exposure to complex mixtures of environmental contaminants contribute to T2DM development. This study examined the association between complex environmental contaminant mixtures and T2DM among Canadian Indigenous communities from the Eeyou Istchee territory, Quebec, Canada. Using data from the cross-sectional Multi-Community Environment-and-Health Study (2005–2009) Principal Component Analysis (PCA) was used to reduce the dimensionality of the following contaminants: 9-polychlorinated biphenyl congeners; 7-organic pesticides; and 4-metal/metalloids. Following this data reduction technique, we estimated T2DM prevalence ratios (PR) and 95% confidence intervals using modified Poisson regression with robust error variance across derived principal components, adjusting for a priori covariates. For both First Nation adult males (n = 303) and females (n = 419), factor loadings showed dichlorodiphenyltrichloroethane (DDT) and lead (Pb) highly loaded on the second principal component (PC) axis: DDT negatively loaded, and Pb positively loaded. T2DM was significantly associated with PC-2 across all adjusted models. Because PCA produces orthogonal axes, increasing PC-2 scores in the fully adjusted model for females and males showed (PR = 0.84; 95% CI 0.72, 0.98) and (PR = 0.78; 95% CI 0.62, 0.98), respectively. This cross-sectional study suggests that our observed association with T2DM is the result of DDT, and less likely the result of Pb exposure. Further, detectable levels of DDT among individuals may possibly contribute to disease etiology.
Serpent/dGATAb regulates Laminin B1 and Laminin B2 expression during Drosophila embryogenesis
Authors: Töpfer Uwe , Bischoff Maik C. , Bartkuhn Marek , Holz Anne 
Published: November 2019

AbstractTranscriptional regulation of Laminin expression during embryogenesis is a key step required for proper ECM assembly. We show, that in Drosophila the Laminin B1 and Laminin B2 genes share expression patterns in mesodermal cells as well as in endodermal and ectodermal gut primordia, yolk and amnioserosa. In the absence of the GATA transcription factor Serpent, the spatial extend of Laminin reporter gene expression was strongly limited, indicating that Laminin expression in many tissues depends on Serpent activity. We demonstrate a direct binding of Serpent to the intronic enhancers of Laminin B1 and Laminin B2. In addition, ectopically expressed Serpent activated enhancer elements of Laminin B1 and Laminin B2. Our results reveal Serpent as an important regulator of Laminin expression across tissues.
Unveiling the electronic transformations in the semi-metallic correlated-electron transitional oxide Mo8O23
Authors: Nasretdinova V. , Gerasimenko Ya. A. , Mravlje J. , Gatti G. , Sutar P. , Svetin D. , Meden A. , Kabanov V. , Kuntsevich A. Yu. , Grioni M. , Mihailovic D. 
Published: November 2019

AbstractMo8O23is a low-dimensional chemically robust transition metal oxide coming from a prospective family of functional materials, MoO3−x, ranging from a wide gap insulator (x = 0) to a metal (x = 1). The large number of stoichometric compounds with intermediatexhave widely different properties. In Mo8O23, an unusual charge density wave transition has been suggested to occur above room temperature, but its low temperature behaviour is particularly enigmatic. We present a comprehensive experimental study of the electronic structure associated with various ordering phenomena in this compound, complemented by theory. Density-functional theory (DFT) calculations reveal a cross-over from a semi-metal with vanishing band overlap to narrow-gap semiconductor behaviour with decreasing temperature. A buried Dirac crossing at the zone boundary is confirmed by angle-resolved photoemission spectroscopy (ARPES). Tunnelling spectroscopy (STS) reveals a gradual gap opening corresponding to a metal-to-insulator transition at 343 K in resistivity, consistent with CDW formation and DFT results, but with large non-thermal smearing of the spectra implying strong carrier scattering. At low temperatures, the CDW picture is negated by the observation of a metallic Hall contribution, a non-trivial gap structure in STS below ∼170 K and ARPES spectra, that together represent evidence for the onset of the correlated state at 70 K and the rapid increase of gap size below ∼30 K. The intricate interplay between electronic correlations and the presence of multiple narrow bands near the Fermi level set the stage for metastability and suggest suitability for memristor applications.
Dynamic Tuning of a Thin Film Electrocatalyst by Tensile Strain
Authors: Benson Eric E. , Ha Mai-Anh , Gregg Brian. A. , van de Lagemaat Jao , Neale Nathan R. , Svedruzic Drazenka 
Published: November 2019

AbstractWe report the ability to tune the catalytic activities for the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) by applying mechanical stress on a highly n-type doped rutile TiO2 films. We demonstrate through operando electrochemical experiments that the low HER activity of TiO2 can reversibly approach those of the state-of-the-art non-precious metal catalysts when the TiO2 is under tensile strain. At 3% tensile strain, the HER overpotential required to generate a current density of 1 mA/cm2 shifts anodically by 260 mV to give an onset potential of 125 mV, representing a drastic reduction in the kinetic overpotential. A similar albeit smaller cathodic shift in the OER overpotential is observed when tensile strain is applied to TiO2. Results suggest that significant improvements in HER and OER activities with tensile strain are due to an increase in concentration of surface active sites and a decrease in kinetic and thermodynamics barriers along the reaction pathway(s). Our results highlight that strain applied to TiO2 by precisely controlled and incrementally increasing (i.e. dynamic) tensile stress is an effective tool for dynamically tuning the electrocatalytic properties of HER and OER electrocatalysts relative to their activities under static conditions.
Biochemical characterisation of four rhamnosidases from thermophilic bacteria of the genera Thermotoga, Caldicellulosiruptor and Thermoclostridium
Authors: Baudrexl Melanie , Schwarz Wolfgang H. , Zverlov Vladimir V. , Liebl Wolfgang 
Published: November 2019

AbstractCarbohydrate active enzymes are classified in databases based on sequence and structural similarity. However, their function can vary considerably within a similarity-based enzyme family, which makes biochemical characterisation indispensable to unravel their physiological role and to arrive at a meaningful annotation of the corresponding genes. In this study, we biochemically characterised the four related enzymes Tm_Ram106B, Tn_Ram106B, Cb_Ram106B and Ts_Ram106B from the thermophilic bacteria Thermotoga maritima MSB8, Thermotoga neapolitana Z2706-MC24, Caldicellulosiruptor bescii DSM 6725 and Thermoclostridium stercorarium DSM 8532, respectively, as α-l-rhamnosidases. Cobalt, nickel, manganese and magnesium ions stimulated while EDTA and EGTA inhibited all four enzymes. The kinetic parameters such as Km, Vmax and kcat were about average compared to other rhamnosidases. The enzymes were inhibited by rhamnose, with half-maximal inhibitory concentrations (IC50) between 5 mM and 8 mM. The α-l-rhamnosidases removed the terminal rhamnose moiety from the rutinoside in naringin, a natural flavonone glycoside. The Thermotoga sp. enzymes displayed the highest optimum temperatures and thermostabilities of all rhamnosidases reported to date. The four thermophilic and divalent ion-dependent rhamnosidases are the first biochemically characterised orthologous enzymes recently assigned to glycoside hydrolase family 106.
Therapeutic potential of peptides from Ole e 1 in olive-pollen allergy
Authors: Calzada David , Cremades-Jimeno Lucía , Pedro María Ángeles de , Baos Selene , Rial Manuel , Sastre Joaquín , Quiralte Joaquín , Florido Fernando , Lahoz Carlos , Cárdaba Blanca 
Published: November 2019

AbstractOlive-pollen allergy is one of the leading causes of respiratory allergy in Mediterranean countries and some areas of North America. Currently, allergen-specific immunotherapy is the only etiophatogenic treatment. However, this approach is not fully optimal, safe, or effective. Thus, efforts continue in the search for novel immunotherapy strategies, being one of the most promising the use of peptides derived from major allergens. This work tries to determine the therapeutic potential and safety of 5 dodecapeptides derived from the main allergen of olive-pollen allergy, Ole e 1. The immunomodulatory capacity of these peptides was studied using peripheral blood mononuclear cells (PBMCs) obtained from 19 olive-pollen-allergic patients and 10 healthy controls. We determined the capacity of these peptides to inhibit the proliferative response toward olive-pollen allergenic extract and to induce the regulatory cytokines, IL-10 and IL-35. To test the safety and absence of allergenicity of the peptides, the basophil activation was analyzed by flow-cytometry, using peripheral blood. The results showed that two of five peptides inhibited near to 30% the proliferative response against the total olive-pollen allergenic extract in olive-pollen-allergic patients. Inhibition increased to nearly 35% when the 5 peptides were used in combination. In both cases, a statistically significant induction of IL-10 and IL-35 secretion was observed in the supernatants of allergic patients PBMCs cultures. None of the 5 peptides induced basophil activation and cross-link inflammatory cell-bound IgE. In conclusion, these results open up new possibilities in the treatment of olive-pollen allergy, which could solve some of the problems facing current therapy approaches.
N-GlyDE: a two-stage N-linked glycosylation site prediction incorporating gapped dipeptides and pattern-based encoding
Authors: Pitti Thejkiran , Chen Ching-Tai , Lin Hsin-Nan , Choong Wai-Kok , Hsu Wen-Lian , Sung Ting-Yi 
Published: November 2019

AbstractN-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions. Since experimental characterization of glycosites is challenging, glycosite prediction is crucial. Several predictors have been made available and report high performance. Most of them evaluate their performance at every asparagine in protein sequences, not confined to asparagine in the N-X-S/T sequon. In this paper, we present N-GlyDE, a two-stage prediction tool trained on rigorously-constructed non-redundant datasets to predict N-linked glycosites in the human proteome. The first stage uses a protein similarity voting algorithm trained on both glycoproteins and non-glycoproteins to predict a score for a protein to improve glycosite prediction. The second stage uses a support vector machine to predict N-linked glycosites by utilizing features of gapped dipeptides, pattern-based predicted surface accessibility, and predicted secondary structure. N-GlyDE’s final predictions are derived from a weight adjustment of the second-stage prediction results based on the first-stage prediction score. Evaluated on N-X-S/T sequons of an independent dataset comprised of 53 glycoproteins and 33 non-glycoproteins, N-GlyDE achieves an accuracy and MCC of 0.740 and 0.499, respectively, outperforming the compared tools. The N-GlyDE web server is available at http://bioapp.iis.sinica.edu.tw/N-GlyDE/.
Towards elucidating the radiochemistry of astatine – Behavior in chloroform
Authors: Aneheim Emma , Palm Stig , Jensen Holger , Ekberg Christian , Albertsson Per , Lindegren Sture 
Published: November 2019

AbstractTargeted alpha therapy of disseminated cancer is an emerging technique where astatine-211 is one of the most promising candidate nuclides. Although astatine has been known for over 70 years, its chemistry is still largely unexplored, mainly due to the lack of stable or long-lived isotopes. However, substantial amounts of astatine-211 can be produced in cyclotrons by the bombardment of natural bismuth. The astatine can be recovered from the resulting irradiated target material through either wet extraction or dry-distillation. Chloroform has become an important intermediate solvent for the recovery of astatine after production, especially following dry distillation. In this work, the radiochemistry of astatine in chloroform was investigated using evaporation, solvent extraction, chromatographic methods and molecular modeling. The extraction of astatine in chloroform led to the formation of multiple astatine species, allowing for evaporation of the solvent to dryness without any loss of activity. Radiolysis products of chloroform were shown to play an important role in the speciation of astatine forming both reactive and kinetically stable compounds. It was hypothesized that reactions with chlorine, as well as trichloromethyl hydroperoxide, forming polar astatine compounds are important reactions under the current experimental conditions.
Superior properties in room-temperature colloidal-dot quantum emitters revealed by ultralow-dark-count detections of temporally-purified single photons
Authors: Ihara Toshiyuki , Miki Shigehito , Yamada Toshiki , Kaji Takahiro , Otomo Akira , Hosako Iwao , Terai Hirotaka 
Published: November 2019

AbstractThe realization of high-quality quantum emitters that can operate at room temperature is important for accelerating the application of quantum technologies, such as quantum communication, quantum information processing, and quantum metrology. In this work, we study the photon-antibunching properties on room-temperature emission from individual colloidal quantum dots (CQDs) using superconducting-nanowire single-photon detectors and temporal filtering of the photoluminescence decay curve. We find that high single-photon purities and high photon-generation rates can be simultaneously achieved by removing the signals originating from the sequential two-photon emission of biexcitons created by multiple excitation pulses. We successfully demonstrate that the ultrahigh performance of the room-temperature single-photon sources showing g(2)(0) ≪ 10−2 can be confirmed by the ultralow-dark-count detection of the temporally purified single photons. These findings provide strong evidence for the attractiveness of CQDs as candidates for high-quality room-temperature quantum light sources.
Multi-symplectic integrator of the generalized KdV-type equation based on the variational principle
Authors: Wei Yi , Zhang Xing-Qiu , Shao Zhu-Yan , Gao Jian-Qiang , Yang Xiao-Feng 
Published: November 2019

AbstractThe variational principle is used to construct a multi-symplectic structure of the generalized KdV-type equation. Accordingly, the local energy conservation law, the local momentum conservation law, and the Cartan form of the generalized KdV-type equation are given. An explicit multi-symplectic scheme for the generalized KdV equation based on the Fourier pseudo-spectral method and the symplectic Euler scheme is constructed. Through a numerical examination, the explicit multi-symplectic Fourier pseudo-spectral scheme for the generalized KdV equation not only preserve the discrete global energy conservation law and the global momentum conservation law with high accuracy, but show long-time numerical stability as well.
Typeface Reveals Spatial Economical Patterns
Authors: Ma Ruixian , Wang Wei , Zhang Fan , Shim Kyuha , Ratti Carlo 
Published: November 2019

AbstractUnderstanding the socioeconomic and demographic characteristics of an urban region is vital for policy-making, urban management, and urban planning. Auditing socioeconomic and demographic patterns traditionally entails producing a large portion of data by human-participant surveys, which are usually costly and time consuming. Even with newly developed computational methods, amenity characteristics such as typeface, color, and graphic element choices are still missing at the city scale. However, they have a huge impact on personalized preferences. Currently, researchers tend to use large-scale street view imagery to uncover physical and socioeconomic patterns. In this research, we first propose a framework that uses deep convolutional neural network to recognize the typeface from street view imagery in London. Second, we analyze the relationship between 11 typefaces and the average household income in 77 wards of London. The result show that the typefaces used in the neighborhood are highly correlated with economic and demographic factors. Typeface could be an alternative metric to evaluate economic and demographic status in large-scale urban regions. More generally, typeface can also act as a key visual characteristic of a city.
Effects of modified aerobic training on muscle metabolism in individuals with peripheral arterial disease: a randomized clinical trial
Authors: Monteiro Débora Pantuso , Ribeiro-Samora Giane Amorim , Britto Raquel Rodrigues , Pereira Danielle Aparecida Gomes 
Published: November 2019

AbstractThe primary objective of this study was to compare the effects on muscle metabolism of two types of aerobic training, with and without a load on the lower limbs, in adults with peripheral arterial disease (PAD). A simple blind randomized clinical trial was conducted using two groups: conventional aerobic (CG) and modified aerobic with a load on the lower limbs (MG). Both groups underwent training by walking three times a week over a 12-week period. The ratings of muscle metabolism were determined after a treadmill test with constant velocity and inclination concomitant with the use of near infrared spectroscopy (NIRS). Altogether 40 individuals with PAD (CG = 65.45 ± 10.60 and MG = 63.10 ± 10.54) were included in the study. After the intervention, in both groups, there was a reduction in the relative time to recovery (p = 0.002), an improvement in the re-oxygenation rate (p = 0.017), an increased time of resistance after reaching the lowest muscle oxygen saturation (StO2) (p < 0.001), an increase in the distance walked (p < 0.001), and an improvement of the walking economy relative to StO2 (p < 0.001). After 12 weeks of training, an improvement in the deoxygenation rate was observed in both groups (p = 0.002), but with a greater magnitude in the CG (p = 0.017). Only the CG presented an increase in time to reach the lowest StO2 on the treadmill after the intervention (p = 0.010). The traditional aerobic training was superior to the modified training in relation to the improvement of muscle metabolism in patients with PAD.
Unraveling oxidative stress response in the cestode parasite Echinococcus granulosus
Authors: Cancela Martín , Paes Jéssica A. , Moura Hercules , Barr John R. , Zaha Arnaldo , Ferreira Henrique B. 
Published: November 2019

AbstractCystic hydatid disease (CHD) is a worldwide neglected zoonotic disease caused byEchinococcus granulosus. The parasite is well adapted to its host by producing protective molecules that modulate host immune response. An unexplored issue associated with the parasite’s persistence in its host is how the organism can survive the oxidative stress resulting from parasite endogenous metabolism and host defenses. Here, we used hydrogen peroxide (H2O2) to induce oxidative stress inE. granulosusprotoescoleces (PSCs) to identify molecular pathways and antioxidant responses during H2O2exposure. Using proteomics, we identified 550 unique proteins; including 474 in H2O2-exposed PSCs (H-PSCs) samples and 515 in non-exposed PSCs (C-PSCs) samples. Larger amounts of antioxidant proteins, including GSTs and novel carbonyl detoxifying enzymes, such as aldo-keto reductase and carbonyl reductase, were detected after H2O2exposure. Increased concentrations of caspase-3 and cathepsin-D proteases and components of the 26S proteasome were also detected in H-PSCs. Reduction of lamin-B and other caspase-substrate, such as filamin, in H-PSCs suggested that molecular events related to early apoptosis were also induced. We present data that describe proteins expressed in response to oxidative stress in a metazoan parasite, including novel antioxidant enzymes and targets with potential application to treatment and prevention of CHD.