bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding pre-operative staging and surgical practice in advanced endometriosis: A survey of Canadian gynaecologists
Authors: Nica Andra , Liu Grace Y. , Selk Amanda , Kroft Jamie 
Published: June 2020

Study objective: To determine the pre-operative evaluation, surgical management and referral practices in patients with advanced stage endometriosis by Canadian gynaecologists. Design: A survey of obstetricians and gynaecologists. Setting: The survey was initiated and piloted at an academic centre by general gynaecologists and endometriosis specialists. Intervention: Electronically distributed to 733 individuals by the Society of Obstetricians and Gynaecologists of Canada. This included all members, irrespective of subspecialty and practice patterns. Measurement: Responses were collected using a web-based survey tool and analysed using Excel. Results: The response rate was 15.7% (115 respondents). Pre-operatively, 62.2% of respondents perform a transvaginal ultrasound on all of their patients, while magnetic resonance imaging is reserved for patients with physical exam findings suspicious for advanced endometriosis (26.7%) or in whom the surgeons suspect deep infiltrating endometriosis, bowel, bladder or uterosacral disease (54.4%). Most surgeons (81.4%) report encountering advanced disease that they did not suspect pre-operatively <10% of the time. Although 40% of respondents would refer their patients in whom they suspected deep infiltrating endometriosis, endometriomas, bowel, bladder or uterosacral ligament involvement to an endometriosis specialist prior to any attempted surgery, 54.4% would never refer without previously confirming the diagnosis at laparoscopy. In contrast, only 15% felt comfortable treating advanced endometriosis completely at time of laparoscopy (including deep infiltrating endometriosis, bladder and bowel disease). Post-operatively, 67.8% of respondents refer patients to an endometriosis specialist only if their disease was not appropriately treated surgically, while 23.3% do not refer any of their patients. Conclusion: Our study identified significant variability in the management of advanced endometriosis in Canada. Understanding these patterns will help us formulate a more universal investigation and management plan, which may improve the identification of patients pre-operatively with advanced stage endometriosis that could benefit from treatment by an endometriosis specialist.
COVID-19 and Inequities in Oral Health Care for Older People: An Opportunity for Emerging Paradigms
Authors: León S. , Giacaman R.A. 
Published: June 2020

Severe restrictions have been imposed in most countries for oral health care to the general population, allowing treatment only for emergencies, because of the generation of aerosols during clinical procedures and to ration personal protective equipment (PPE) during the pandemic. This article stresses the critical situation that affects oral health for older persons in the complex times of the COVID-19 pandemic. Older adults are at high risk for the viral infection, but neglected dental conditions may worsen their health, triggering infections that can lead to local and systemic complications, compromising general health. We propose that under current and possibly future scenarios, teledentistry and the minimal intervention dentistry approach may play a pivotal role in reshaping the profession. The already existent inequities in oral health care may be exacerbated due to the pandemic, especially in the developing world. More research along with a strong educational component in the dental curriculum must be emphasized. COVID-19 may be an opportunity to change canonical paradigms. The dental profession must reflect and take action to face future challenges. Knowledge Transfer Statement: This article provides an overview of the oral health situation imposed by COVID-19 and the minimal intervention alternatives to provide care to older people who are at risk and have reduced access to care.
Diagnosis of deep vein thrombosis of the upper extremity: a systematic review and meta-analysis of test accuracy
Authors: Patel Payal , Braun Cody , Patel Parth , Bhatt Meha , Begum Housne , Wiercioch Wojtek , Varghese Jamie , Wooldridge David , Alturkmani Hani J. , Thomas Merrill , Baig Mariam , Bahaj Waled , Khatib Rasha , Kehar Rohan , Ponnapureddy Rakesh , Sethi Anchal , Mustafa Ahmad , Lim Wendy , Le Gal Grégoire , Bates Shannon M. , Lang Eddy , Righini Marc , Husainat Nedaa M. , Kalot Mohamad A. , Al Jabiri Yazan Nayif , Nieuwlaat Robby , Schünemann Holger J. , Mustafa Reem A. 
Published: June 2020

Abstract
Upper extremity deep vein thrombosis (UEDVT) accounts for ≤10% of DVT and can be associated with morbidity and mortality. Accurate diagnosis and treatment are necessary for safe and effective patient management. We systematically reviewed the accuracy of D-dimer and duplex ultrasonography (US) for the evaluation of suspected first-episode UEDVT. We searched the Cochrane Central Register, OVID MEDLINE, EMBASE, and PubMed for eligible studies, reference lists of relevant reviews, registered trials, and relevant conference proceedings. We included prospective cross-sectional and cohort studies that evaluated test accuracy. Two investigators independently screened and collected data. The risk of bias was assessed using Quality Assessment of Diagnostic Accuracy Studies 2 and certainty of evidence using the Grading of Recommendations Assessment, Development and Evaluation framework. We pooled estimates of sensitivity and specificity. The review included 9 studies. The pooled estimates for D-dimer sensitivity and specificity were 0.96 (95% confidence interval [CI], 0.87-0.99) and 0.47 (95% CI, 0.43-0.52), respectively. The pooled estimates for duplex US sensitivity and specificity were 0.87 (95% CI, 0.73-0.94) and 0.85 (95% CI, 0.72-0.93), respectively. Certainty of evidence was moderate. In this review, we summarized the test accuracy (sensitivity and specificity) of D-dimer and duplex US for this indication. The sensitivity and specificity of the tests found in the present review should be considered in the context of whether they are used alone or in combination, which is dependent on the prevalence of disease in the population, the clinical setting in which the patient is being evaluated, cost, potential harms, and patient outcomes. This study was registered at PROSPERO as Systematic Review Registration Number CRD42018098488.
Transient antibody targeting of CD45RC inhibits the development of graft-versus-host disease
Authors: Boucault Laetitia , Lopez Robles Maria-Dolores , Thiolat Allan , Bézie Séverine , Schmueck-Henneresse Michael , Braudeau Cécile , Vimond Nadège , Freuchet Antoine , Autrusseau Elodie , Charlotte Frédéric , Redjoul Rabah , Beckerich Florence , Leclerc Mathieu , Piaggio Eliane , Josien Regis , Volk Hans-Dieter , Maury Sébastien , Cohen José L. , Anegon Ignacio , Guillonneau Carole 
Published: June 2020

Abstract
Allogeneic bone marrow transplantation (BMT) is a widely spread treatment of many hematological diseases, but its most important side effect is graft-versus-host disease (GVHD). Despite the development of new therapies, acute GVHD (aGVHD) occurs in 30% to 50% of allogeneic BMT and is characterized by the generation of effector T (Teff) cells with production of inflammatory cytokines. We previously demonstrated that a short anti-CD45RC monoclonal antibody (mAb) treatment in a heart allograft rat model transiently decreased CD45RChigh Teff cells and increased regulatory T cell (Treg) number and function allowing long-term donor-specific tolerance. Here, we demonstrated in rat and mouse allogeneic GVHD, as well as in xenogeneic GVHD mediated by human T cells in NSG mice, that both ex vivo depletion of CD45RChigh T cells and in vivo treatment with short-course anti-CD45RC mAbs inhibited aGVHD. In the rat model, we demonstrated that long surviving animals treated with anti-CD45RC mAbs were fully engrafted with donor cells and developed a donor-specific tolerance. Finally, we validated the rejection of a human tumor in NSG mice infused with human cells and treated with anti-CD45RC mAbs. The anti-human CD45RC mAbs showed a favorable safety profile because it did not abolish human memory antiviral immune responses, nor trigger cytokine release in in vitro assays. Altogether, our results show the potential of a prophylactic treatment with anti-human CD45RC mAbs in combination with rapamycin as a new therapy to treat aGVHD without abolishing the antitumor effect.
Risks for cold frequency vary by sex: role of asthma, age, TLR7 and leukocyte subsets
Authors: Murray Liisa M. , Yerkovich Stephanie T. , Ferreira Manuel A. , Upham John W. 
Published: June 2020

Viral respiratory infections are usually benign but can trigger asthma exacerbations. The factors associated with upper respiratory tract infection (cold) frequency are not fully understood, nor is it clear whether such factors differ between women and men.To determine which immunological and clinical variables associate with the frequency of self-reported respiratory infections (colds), 150 asthma cases and 151 controls were recruited. Associations between antiviral immune response variables: toll-like receptor (TLR)7/8 gene expression, plasmacytoid dendritic cell (pDC) numbers and interferon-α, tumour necrosis factor and interleukin-12 production, and asthma were then examined that might explain cold frequency.People with asthma cases reported more colds per year (median 3versus2; p<0.001) and had lower baseline TLR7 gene expression (odds ratio 0.12; p=0.02) than controls. Associations between many variables and cold frequency differed between women and men. In women, high blood neutrophil counts (β=0.096, p=0.002), and younger age (β=−0.017, p<0.001), but not exposure to children, were independently associated with more frequent colds. In men, low TLR7 expression (β=−0.96, p=0.041) and high CLEC4C gene expression (a marker of pDC; β=0.88, p=0.008) were independently associated with more frequent colds. Poor asthma symptom control was independently associated with reduced TLR8 gene expression (β=−1.4, p=0.036) and high body mass index (β=0.041, p=0.004).Asthma, age and markers of inflammation and antiviral immunity in peripheral blood are associated with frequent colds. Interestingly, the variables associated with cold frequency differed between women and men.
Variability in airway inflammation, symptoms, lung function and reliever use in asthma: anti-inflammatory reliever hypothesis and STIFLE study design
Authors: Harrison Tim , Pavord Ian D. , Chalmers James D. , Whelan Glenn , Fagerås Malin , Rutgersson Annika , Belton Laura , Siddiqui Shahid , Gustafson Per 
Published: June 2020

Asthma is a chronic inflammatory airway disease. Increase in airway inflammation is hypothesised to contribute to worsening of asthma symptoms and deterioration in lung function, resulting in the use of reliever medication. Short-acting β2-agonists only treat the symptoms, whereas an anti-inflammatory reliever is believed to treat both symptoms and the underlying inflammation, thereby arresting the progression to an exacerbation. As-needed budesonide/formoterol as an anti-inflammatory reliever reduces the risk of severe exacerbations. However, supporting mechanistic evidence has not yet been described, specifically the temporal dynamics of parameters including airway inflammation, over time and during asthma worsening.The STIFLE study aims to characterise daily variability in airway inflammation, symptoms, lung function and reliever use in people with asthma. This phase IV, open-label, parallel-group, multicentre, exploratory study will enrol 60–80 adult patients with asthma receiving low- or medium-dose inhaled corticosteroids/long-acting β2-agonists (EudraCT identifier number 2018-003467-64). Participants will be randomised 1:1 to either as-needed budesonide/formoterol dry-powder inhaler or salbutamol reliever for 24 weeks, in addition to their maintenance therapy. Daily data will be captured for fractional exhaled nitric oxide, spirometry, asthma symptoms and medication use using devices connected to a smartphoneviathe STIFLE application. STIFLE will thereby enable not only characterisation of the variability of airway inflammation and clinical outcomes in relation to asthma worsening, but also elucidate the effect of as-needed budesonide/formoterol on airway inflammation against a background of daily maintenance therapy.
Integrated Modelling of Microstructure Evolution and Mechanical Properties Prediction for Q&P Hot Stamping Process of Ultra-High Strength Steel
Authors: Chen Yang , Zhang Huizhen , Tang Johnston Jackie , Han Xianhong , Cui Zhenshan 
Published: June 2020

AbstractHigh strength steel products with good ductility can be produced via Q&P hot stamping process, while the phase transformation of the process is more complicated than common hot stamping since two-step quenching and one-step carbon partitioning processes are involved. In this study, an integrated model of microstructure evolution relating to Q&P hot stamping was presented with a persuasively predicted results of mechanical properties. The transformation of diffusional phase and non-diffusional phase, including original austenite grain size individually, were considered, as well as the carbon partitioning process which affects the secondary martensite transformation temperature and the subsequent phase transformations. Afterwards, the mechanical properties including hardness, strength, and elongation were calculated through a series of theoretical and empirical models in accordance with phase contents. Especially, a modified elongation prediction model was generated ultimately with higher accuracy than the existed Mileiko’s model. In the end, the unified model was applied to simulate the Q&P hot stamping process of a U-cup part based on the finite element software LS-DYNA, where the calculated outputs were coincident with the measured consequences.
Effect of desk materials on affective states and cognitive performance
Authors: Lipovac Dean , Podrekar Nastja , Burnard Michael D. , Šarabon Nejc 
Published: June 2020

AbstractVisual and tactile properties of materials can influence human emotional and cognitive functioning. Existing studies indicate that exposure to wood may lead to more favourable outcomes than contact with other common materials, but evidence is limited. We measured affective states and cognitive performance in 16 participants before and after each spent 15 min at 10 desks with differing top surfaces. Desk surfaces were made of untreated, oiled, or lacquered spruce or oak solid wood, laminated or oak-veneered particleboard, glass, and mineral-filled thermoplastic composite. The results indicate that cognitive performance and affective states of participants did not differ between the desk surfaces. It appears that exposure to a relatively small wooden surface does not significantly influence affective and cognitive outcomes. Incorporating larger amounts of wood coverage and a more demanding cognitive task would probably increase the chances of capturing the potential effects of wood exposure on human affective states and cognitive performance.
Vehicle energy savings by optimizing road speed-sectioning
Authors: Coiret Alex , Deljanin Emir , Vandanjon Pierre-Olivier 
Published: June 2020

AbstractIn a context of decreasing resources and of climate change, lowering road vehicles consumption is a key point to meet CO2reduction requirements. In addition to car technological advances, eco-driving is part of the solution but the road infrastructure should ensure its development. This work aims to demonstrate that road energy demand and associated pollutant emissions can be reduced by working out minor optimization of road infrastructure itself. For this to happen, a simple eco-driving potential criterion is built upon infrastructure parameters such as slopes and sight distances. This criterion aims to detect Misplaced Speed-sectioning Positions (MSP) with regard to the Starting Point of Deceleration (SPD); with speed-sectioning being the succession of speed changes along a given route. An enhanced energy waste formulation is then developed to quantify the vehicles energy waste due to misplaced road-signs. Thirdly, a traffic simulation constitutes a framework for energy evaluation; considering a full flow of vehicles, based on real traffic data, and by modeling several driver behaviors. Simulation results show that a significant fuel reduction of up to 5.5% can be achieved locally, simply by moving a road sign, for rural areas and without degrading road safety.
An improved clear cell renal cell carcinoma stage prediction model based on gene sets
Authors: Li Fangjun , Yang Mu , Li Yunhe , Zhang Mingqiang , Wang Wenjuan , Yuan Dongfeng , Tang Dongqi 
Published: June 2020

Abstract
Background
Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cell carcinoma and accounts for cancer-related deaths. Survival rates are very low when the tumor is discovered in the late-stage. Thus, developing an efficient strategy to stratify patients by the stage of the cancer and inner mechanisms that drive the development and progression of cancers is critical in early prevention and treatment.

Results
In this study, we developed new strategies to extract important gene features and trained machine learning-based classifiers to predict stages of ccRCC samples. The novelty of our approach is that (i) We improved the feature preprocessing procedure by binning and coding, and increased the stability of data and robustness of the classification model. (ii) We proposed a joint gene selection algorithm by combining the Fast-Correlation-Based Filter (FCBF) search with the information value, the linear correlation coefficient, and variance inflation factor, and removed irrelevant/redundant features. Then the logistic regression-based feature selection method was used to determine influencing factors. (iii) Classification models were developed using machine learning algorithms. This method is evaluated on RNA expression value of clear cell renal cell carcinoma derived from The Cancer Genome Atlas (TCGA). The results showed that the result on the testing set (accuracy of 81.15% and AUC 0.86) outperformed state-of-the-art models (accuracy of 72.64% and AUC 0.81) and a gene set FJL-set was developed, which contained 23 genes, far less than 64. Furthermore, a gene function analysis was used to explore molecular mechanisms that might affect cancer development.

Conclusions
The results suggested that our model can extract more prognostic information, and is worthy of further investigation and validation in order to understand the progression mechanism.
Effects of CO2 limitation on the metabolism of Pseudoclostridium thermosuccinogenes
Authors: Koendjbiharie Jeroen Girwar , Post Wilbert Berend , Palmer Martí Munar , van Kranenburg Richard 
Published: June 2020

Abstract
Background
Bio-based succinic acid holds promise as a sustainable platform chemical. Its production through microbial fermentation concurs with the fixation of CO2, through the carboxylation of phosphoenolpyruvate. Here, we studied the effect of the available CO2 on the metabolism of Pseudoclostridium thermosuccinogenes, the only known succinate producing thermophile. Batch cultivations in bioreactors sparged with 1 and 20% CO2 were conducted that allowed us to carefully study the effect of CO2 limitation.

Results
Formate yield was greatly reduced at low CO2 concentrations, signifying a switch from pyruvate formate lyase (PFL) to pyruvate:ferredoxin oxidoreductase (PFOR) for acetyl-CoA formation. The corresponding increase in endogenous CO2 production (by PFOR) enabled succinic acid production to be largely maintained as its yield was reduced by only 26%, thus also maintaining the concomitant NADH re-oxidation, essential for regenerating NAD+ for glycolysis. Acetate yield was slightly reduced as well, while that of lactate was slightly increased. CO2 limitation also prompted the formation of significant amounts of ethanol, which is only marginally produced during CO2 excess. Altogether, the changes in fermentation product yields result in increased ferredoxin and NAD+ reduction, and increased NADPH oxidation during CO2 limitation, which must be linked to reshuffled (trans) hydrogenation mechanisms of those cofactors, in order to keep them balanced. RNA sequencing, to investigate transcriptional effects of CO2 limitation, yielded only ambiguous results regarding the known (trans) hydrogenation mechanisms.

Conclusions
The results hinted at a decreased NAD+/NADH ratio, which could ultimately be responsible for the stress observed during CO2 limitation. Clear overexpression of an alcohol dehydrogenase (adhE) was observed, which may explain the increased ethanol production, while no changes were seen for PFL and PFOR expression that could explain the anticipated switch based on the fermentation results.
NaCl improves reproduction by enhancing starch accumulation in the ovules of the euhalophyte Suaeda salsa
Authors: Guo Jianrong , Du Ming , Lu Chaoxia , Wang Baoshan 
Published: June 2020

Abstract
Background
Halophytes show optimal reproduction under high-salinity conditions. However, the role of NaCl in reproduction and its possible mechanisms in the euhalophyte Suaeda salsa remain to be elucidated.

Results
We performed transcript profiling of S. salsa flowers and measured starch accumulation in ovules, sugar contents in flowers, and photosynthetic parameters in the leaves of plants supplied with 0 and 200 mM NaCl. Starch accumulation in ovules, sugar contents in flowers and ovules, and net photosynthetic rate and photochemical efficiency in leaves were significantly higher in NaCl-treated plants vs. the control. We identified 14,348 differentially expressed genes in flowers of NaCl-treated vs. control plants. Many of these genes were predicted to be associated with photosynthesis, carbon utilization, and sugar and starch metabolism. These genes are crucial for maintaining photosystem structure, regulating electron transport, and improving photosynthetic efficiency in NaCl-treated plants. In addition, genes encoding fructokinase and sucrose phosphate synthase were upregulated in flowers of NaCl-treated plants.

Conclusions
The higher starch and sugar contents in the ovules and flowers of S. salsa in response to NaCl treatment are likely due to the upregulation of genes involved in photosynthesis and carbohydrate metabolism, which increase photosynthetic efficiency and accumulation of photosynthetic products under these conditions.