bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zoonotic potential of Enterocytozoon bieneusi in pre-weaned Korean native calves
Authors: Hwang Sunwoo , Shin Seung-Uk , Kim SuHee , Ryu Ji-Hyoung , Choi Kyoung-Seong 
Published: May 2020

Abstract
Background: Enterocytozoon bieneusi is the most common microsporidian species that can infect humans and various animals worldwide. To date, there has been limited information on the prevalence and genotypes of E. bieneusi infection in cattle in the Republic of Korea. Therefore, this study investigated the prevalence and genotypes of E. bieneusi circulating in pre-weaned Korean native calves and determined the age pattern of E. bieneusi infection and the relationship between E. bieneusi infection and diarrhea. Methods: The prevalence of E. bieneusi infection in pre-weaned Korean native calves was screened by polymerase chain reaction. PCR-positive products were sequenced to determine the genotype of E. bieneusi . A Chi-square analysis was used to compare the association between diarrhea and the infection rate of E. bieneusi in each age ranges or all ages. Results: PCR and sequencing analysis revealed an overall prevalence of E. bieneusi of 16.9% (53/314) in pre-weaned calves. The prevalence of E. bieneusi was highest in September (36.2%), followed by March (28.3%). E. bieneusi infection (χ 2 = 5.82, P = 0.016) was associated with diarrhea in calves. Our results also indicated that E. bieneusi infection was statistically associated with calf age (χ 2 = 11.61, P = 0.003), and the prevalence of E. bieneusi infection was significantly higher in calves aged 21-40 days (odds ratio = 2.90, 95% confidence interval: 1.54-5.45; P = 0.001) than in those aged 1-20 days. Interestingly, the association between E. bieneusi infection and diarrhea was observed only in calves aged 1-20 days (χ 2 = 5.82, P = 0.010). Furthermore, our results indicated that E. bieneusi infection in pre-weaned calves may be associated with mild diarrhea rather than severe diarrhea. We identified three known genotypes, BEB4 (n = 12), BEB8 (n = 21), and J (n = 16), and three novel genotypes, BEB8-like (n = 21), KCALF1 (n = 1), and KCALF2 (n = 1); all of which belonged to Group 2. The genotype BEB8 was the most prevalent among all age groups, irrespective of diarrhea. All genotypes identified in this study exhibited zoonotic potential. Conclusions: To our knowledge, this is the first report of the presence of the genotype BEB4 in pre-weaned Korean native calves. Zoonotic E. bieneusi infection was prevalent in pre-weaned Korean native calves, indicating that cattle may play an important role as a reservoir host for E. bieneusi transmission to humans.
Linc00473 potentiates cholangiocarcinoma progression by modulation of DDX5 expression via miR-506 regulation
Authors: Huang Lining , Jiang Xingming , Li Zhenglong , Li Jinglin , Lin Xuan , Hu Zengtao , Cui Yunfu 
Published: May 2020

Abstract
Background: Cholangiocarcinoma (CCA) is a mortal cancer with high mortality, whereas the function and mechanism of occurrence and progression of CCA are still mysterious. Long non-coding RNAs (lncRNAs) could function as important regulators in carcinogenesis and cancer progression. Growing evidences have indicated that the novel lncRNA linc00473 plays an important role in cancer progression and metastasis. However, its function and molecular mechanism in CCA remain unknown. Methods: The linc00473 expression in CCA tissues and cell lines was analyzed using qRT-PCR. Gain- and loss-of-function experiments were conducted to investigate the biological functions of linc00473 both in vitro and in vivo. Insights into the underlying mechanisms of competitive endogenous RNAs (ceRNAs) were determined by bioinformatics analysis, dual-luciferase reporter assays, qRT-PCR arrays, RNA immunoprecipitation (RIP) and rescue experiments. Results: Linc00473 was highly expressed in CCA tissues and cell lines. Linc00473 knockdown inhibited CCA growth and metastasis. Furthermore, linc00473 acted as miR-506 sponge and regulated its target gene DDX5 expression. Rescue assays verified that linc00473 modulated the tumorigenesis of CCA by regulating miR-506. Conclusions: The data indicated that linc00473 played an oncogenic role in CCA growth and metastasis, and could serve as a novel molecular target for treating CCA.
Research on Underground Location Algorithm Based on Random Forest and Environmental Factor Compensation
Authors: Qiao Xin , Chang Fei 
Published: May 2020

Abstract
In view of the complex and changeable underground environment of coal mine and the long strip shape of underground roadway, a new underground location algorithm based on Stochastic Forest and environmental factor compensation is proposed. Firstly, the underground AP network model and roadway environment are analyzed, and the fingerprint localization algorithm is constructed. At the same time, the Kalman filter algorithm is used to filter the RSS signal in the offline sampling and real-time positioning stage. Then the algorithm based on random forest and environmental factor compensation is proposed. Under the assumption that the attenuation factors between the two anchor nodes are the same, the signal strength ratio compensation algorithm is proposed, which optimizes the shortcomings of the similarity of the locality error of the similar region. The target speed constraint condition is introduced to reduces errors caused by the transmission of RSS signal transmission and environmental factors. Experiments were carried out by using a bomb shelter to simulate the real mine roadway environment. The results show that the proposed algorithm can meet the high-precision positioning of the well under the conditions of sparse anchor nodes and complex environment underground.
How different is similar – exploring development of Ceropegia sandersonii pitfall flowers to elucidate convergent evolution of floral synorganization
Authors: Heiduk Annemarie , Pramanik Dewi , Spaans Marlies , Gast Loes , Dorst Nemi , Welten Monique , Gravendeel Barbara 
Published: May 2020

Abstract
Background: Lantern plants from the genus Ceropegia (Apocynaceae-Asclepiadoideae) have radially symmetric pitfall flowers that are an outstanding example of functional floral complexity with high synorganization of specialized organs. The evolutionary origin and development of these complex flowers is unclear, and the genetic background of floral organ formation is unknown. Flowers with similar deceptive pollination strategies and floral traits convergently evolved in non-related plant lineages. The partially bilaterally flattened trap flowers of pipevines are functionally similar to Ceropegia pitfall flowers; many orchid taxa evolved complex fully bilaterally flattened flowers with specialized organs to trap pollinators. This study is the first to investigate the genetic background of pitfall flower development in Ceropegia, and to explore (i) convergent evolution of extremely synorganized and complex flowers as well as (ii) the homology of a highly specialized floral organ, the gynostegial corona. Methods: We obtained transcriptomes from C. sandersonii early floral buds and mature sepals, petals, and gynostegia, and analyzed differential expression of selected MADS-box genes in buds and mature floral organs using RT-PCR. In addition, we studied floral ontogeny and vascularization using SEM and 3D X-ray micro-CT scanning. Results: We identified ten phases of floral development from primordia to mature flowers, and for the first time visualized the vascular system of mature Ceropegia pitfall flowers in a 3D-model. We identified 14 MADS-box gene homologs, representing all major MADS-box gene classes, in the floral transcriptomes of Ceropegia. Vascular bundle patterns, as revealed by 3D X-ray micro-CT scanning, in combination with high expression of GLOBOSA and AGAMOUS indicate a staminoid origin of this highly specialized floral organ which starts developing from stage seven onwards. Interestingly, AGAMOUS-LIKE6 was neither expressed in early floral buds nor in any mature floral organ, in line with the radial symmetry of all Ceropegia floral organs. Conclusion: We detected differential expression of MADS-box genes involved in Ceropegia floral organ identity and propose a new ABCDE-model for parachute flowers. We compare this with current models of unrelated plants with similar floral traits but (partially) bilaterally flattened flowers, i.e. Aristolochia fimbriata and Erycina pusilla. With this comparative approach we lay the foundation for understanding the genetic mechanisms driving convergent evolution of highly specialized deceptive trap flowers.
Heterogeneity of luminal A and HER2-positive breast cancer based on DIA quantitative proteomics and bioinformatics analysis techniques
Authors: Wu Guogang , Zhang Lei , Liang Chunyan , Cheng Yao , Lv Chenguang , Chen Bo 
Published: May 2020

Abstract
Background: Breast cancer has common tumor biological characteristics, but different characteristics also exist among different subtypes. Different treatment strategies have been adopted for different subtypes at present, but there are still many problems. Thus, an-depth study on the heterogeneity of different types of breast cancer will help to identify new diagnostic therapeutic targets and solve the existing problems of individualized treatment for breast cancer.Methods: In this study proteins of HER2-positive breast cancer, luminal A breast cancer, and para-cancer tissues were quantified based on data independent acquisition (DIA) proteomics technology, and the differentially expressed proteins (DEPs) were screened and analyzed by IPA software and the database among the luminal A (LA ) versus para-cancer tissues (PT) and HER2 versus LA groups.Results: There were 264 up- and 123 down-regulated proteins in the LA versus PT groups. PD-1 and PD-L1 cancer immunotherapy pathways were significantly inhibited, and XBP1 was predicted to be the strongest activator. MTOR was predicted to be the strongest inhibitor. The DEPs were significantly associated with “Cancer” and “Organismal Injury and Abnormalities.” There were 134 up- and 286 down-regulated proteins in the HER2 versus LA groups. Signaling by rho family GTPases was significantly inhibited. XBP1 was predicted to be the strongest activator. TGFB1 was predicted to be the strongest inhibitor. The DEPs were significantly associated with “Cancer,” “Endocrine System Disorders,” and “Organismal Injury and Abnormalities.”Conclusion: Luminal A breast cancer may be insensitive to PD-1 and PD-L1 inhibitors. Signaling by rho family GTPases and RhoGDI signaling play a unique role in the proliferation and metastasis of luminal A and HER2-positive breast cancer, respectively. XBP1 is a promising new target for the treatment of breast cancer, and TGFB1 may play different biological roles in HER2-positive and luminal A breast cancer.
Genome-wide Small Rna Profiling Reveals Tiller Development in Tall Fescue (Festuca Arundinacea Schreb.)
Authors: Hu Tao , Wang Tao , Li Huiying , Wassie Misganaw , Chen Liang 
Published: May 2020

Abstract
Background: Tall fescue (Festuca arundinacea Schreb.) is a major cool-season forage and turfgrass species. The low tiller density and size dramatically limits its turf performance and forage yield. MicroRNAs (miRNA)-genes modules play critical roles in tiller development in plants. In this study, a genome-wide small RNA profiling was carried out in two tall fescue genotypes contrasting for tillering production (‘Ch-3’, high tiller production rate and ‘Ch-5’, low tiller production rate) andtwo types of tissue samples at different tillering development stage (Pre-tillering, grass before tillering; Tillering, grass after tillering). ‘Ch-3’, ‘Ch-5’, Pre-tillering, and Tillering samples were analyzed using high-throughput RNA sequencing.Results: A total of 222 million high-quality clean reads were generated and 208 miRNAs were discovered, including 148 known miRNAs belonging to 70 families and 60 novel ones. Furthermore, 18 miRNAs were involved in tall fescue tiller development process. Among them, 14 miRNAs displayed increased abundance in both Ch-3 and Tillering plants compared with that in Ch-5 and Pre-tillering plants and were positive with tillering, while another four miRNAs were negative with tiller development. Out ofthe three miRNAs osa-miR156a, zma-miR528a-3p and osa-miR444b.2, the rest of 15 miRNAs were newfound controllers mediating tiller development in plants. Based on our previous full-length transcriptome analysis in tall fescue, 2 8927 potential target genes were discoveredfor all identified miRNAs. Most of the 212 target genes of the 18 miRNAs were dominantly enriched into “ubiquitin-mediated proteolysis”, “phagosome”, “fatty acid biosynthesis”, “oxidative phosphorylation”, and “biosynthesis of unsaturated fatty acids” KEGG pathways.Conclusions: This is the first genome-wide miRNA profiles analysis to identifyregulators involved in tiller development in cool-season turfgrass. Tillering related 18 miRNAs and their 212 target genes provide novel information for the understanding of the molecular mechanisms of miRNA-genes mediated tiller development in cool-season turfgrass.
Suicides and Macroeconomic Variables: Are They Related in the Long-run?
Authors: Oksak Yuksel , Koyuncu Cuneyt , Yilmaz Rasim 
Published: May 2020

Abstract
BackgroundThis study investigates the cross-country long run relationship between suicides and macroeconomic variables (unemployment, per capita income, and inflation). It is hypothesized that while inflation level and unemployment level stimulate suicide and intentional self-harm in a society, per capita income level alleviates suicide and intentional self-harm in a society.MethodA balanced annual data spanning the period 2000 to 2012 across 35 countries is used in the empirical analysis. We employ panel test and estimation approaches to reveal the long-run association among suicide, inflation, per capita income and unemployment series. The most conventional cross-sectional dependency tests, panel unit root tests, panel cointegration tests, and heterogeneous panel non-causality tests are implemented. ResultsWe found a statistically significant cross-country long run association between suicides and all macroeconomic variables under study. The results of the study suggest that while 1% increase in per capita income causes 0.752% decrease in suicide rate, 1% increase in inflation and unemployment rate is associated with a rise in suicide rate by 0.088% and 0.238%, respectively. In regard to causality, there is no causality is identified between inflation and suicide. On the other hand, a statistically significant unidirectional causality running from per capita income level to suicide and a unidirectional causality running from suicide to unemployment are found. A unidirectional causality running from suicide to unemployment can be stem from the fact that rises in suicides are associated with both early indicators of economic downturns and during economic downturns when unemployment increases.ConclusionHaving found that adverse economic conditions such as increase in unemployment or inflation or decrease in per capita income triggers suicides and suicides are also associated with early indicators of economic crises, this study suggest that social and economic policy measures and programs related to labor market, health safety, family support and debt relief should be implemented both prior to and during economic crises in order to prevent suicides and loss of human capital of the society. Economic policies that result in a high level of unemployment or inflation should be critically assessed from the human cost of these measures.
Older Persons’ Thoughts about Death and Dying and their Experiences of End-of-Life Care: A Qualitative Study
Authors: Tjernberg Johanna , Bökberg Christina 
Published: May 2020

Abstract
Background: Few studies have focused on how older persons living in nursing homes perceive their last period of life. Furthermore, previous research on older persons’ perceptions of death and dying is limited. Hence, there is an urgent need to explore their experiences during their final period in life.Aim: To explore thoughts about death and dying and experiences of end-of-life care among older persons living in nursing homes.Methods: This study employed a qualitative approach including individual interviews with 36 older persons living in Swedish nursing homes. Questions related to quality of life; physical health; thoughts about death, dying, and the future; and experiences related to the living condition and environment were asked. The interview transcripts were analysed through content analysis. The study was approved by the Regional Ethics Review Board (reference number: 2015/4).Results: The analysis resulted in the identification of three main thematic categories: The unavoidable and unknown end of life, Thoughts on control and Living your last period of life at a nursing home. The older persons did not fear death itself but had some worries about dying. Spending the last stage of life at a nursing home contributed to different thoughts and feelings among the older persons. With a few exceptions, older persons characterized life at the nursing home as boring and felt they were surrounded by people who did not belong there.Conclusions: This study indicates a need for older persons to talk about death, dying and end-life issues. Furthermore, this study highlighted that the co-residence of cognitively healthy persons and persons with dementia in the same ward adversely affected cognitively healthy persons. This situation resulted in there being not enough time to both handle the care needs of persons with dementia and have the conversations that cognitively healthy persons desired, such as conversations about thoughts about existence, that could have improved their quality of life.Trial registration: NCT02708498 Date of registration 16 February 2016.
Effects, Barriers and Facilitators in Predischarge Home Assessments to Improve the Transition of Care from the Inpatient Care to Home in Adult Patients: An Integrative Review
Authors: Kirchner-Heklau Uta , Krause Kai , Saal Susanne 
Published: May 2020

Abstract
Background: Predischarge home assessments (PDHA) aim to ensure safe discharge from hospital or rehabilitation. There is insufficient evidence on the effectiveness of PDHA. For adults with any diagnosis, we aimed to determine (1) the effects of PDHA on outcomes associated with the successful return to community living (e.g., Activities of Daily Living falls) and (2) the associated barriers and facilitators to derive recommendations for clinical practice.Methods: We searched Medline, EMBASE, CINAHL, five additional databases and other sources. We included individual and cluster randomized (RCT/cRCT) and controlled clinical trials comparing PDHA versus usual care/other intervention, as well as qualitative/mixed methods studies dealing with PDHA. Critical appraisal was performed according to the Cochrane risk-of-bias tool in quantitative studies and the Critical Appraisal Skills Programme (CASP) for qualitative studies and data extraction. Meta-analysis, thematic synthesis and integrative synthesis were performed.Results: Seven RCTs (n = 1072) and ten qualitative studies (n = 245) met the inclusion criteria. RCTs reported a variety of outcomes (n = 17). There is moderate to very low evidence for missing effects on (instrumental) Activities of Daily Living, quality of life, risk of falling and risk of readmission, mobility and fear of falling. The qualitative studies revealed the following topics related to patient education, patient information, patients’ acceptance of modifications and aids, involvement of patient goals and meaningful activities in functional assessment, as well as relevant social- and diagnosis-related patient conditions in PDHA. Seven implications for interventions were derived from these topics. Six of the included RCTs each addressed at least one and at most three of the seven identified meaningful implications for implementation.Conclusion: There is no evidence from the meta-analysis for the effectiveness of PDHA. Further robust studies are needed to adapt and evaluate PDHA interventions, taking the identified implications from stakeholders’ views into account, and should follow the current recommendations for the development and evaluation of complex interventions.Trial registration: The review was registered and methods were reported on PROSPERO on 18th July 2018 (https://www.crd.york.ac.uk/PROSPERO. CRD42018100636).
Correlation Analysis for the Selection of Microtitanium Plates with Different Specifications for use in a Cervical Vertebral Dome Expansion Laminoplasty
Authors: Run-tian Zhou , Yi-bo Zhao , Xiang-dong Lu , Xiao-feng Zhao , Xiao-nan Wang , Yuan-zhang Jin , Qi De-tai , Zhao Bin 
Published: May 2020

Abstract
Backgrounds: Cervical vertebral dome expansion laminoplasty is a new surgical method for the treatment of cervical spondylosis. We analyzed correlations between the selection of microtitanium plates with different specifications for use in a cervical vertebral dome expansion laminoplasty to establish guidance for the selection of suitable microtitanium plates.Methods: Sixteen patients that underwent the new, full lamina posterior spinal canal enlargement with a cervical spinal stenosis angioplasty procedure for treatment of their cervical spinal cords were recruited at our hospital. From February 2017-September 2018, medical records confirmed that all patients underwent cervical CT and MRI tests pre- and postsurgery. The anteroposterior diameter of the spinal canal, changes in the cross-sectional area of the spinal canal, and the pre- and postsurgery distance of the cervical spinal cord after applying microtitanium plates with different lengths were measured by Mimics version 17.0 software. A statistical regression and correlation analysis of relevant specification parameters of the microtitanium plate was then studied.Results: As the size of the microtitanium plate increased, we found that the cross-sectional area of cervical spinal canal and distance between the descendants of the lamina and the distance of cervical spinal cord concordantly increased. The regression equation associated with sagittal diameter, cross-sectional area, and posterior movement distance of the cervical spinal cord was obtained.Conclusions: The use of the corresponding regression equations enabled the prediction of the cervical spinal canal parameters and posterior movement distance of the cervical spinal cord when adopting different specifications of the microtitanium plate for different segments of the cervical vertebrae. This analysis guided the selection of microtitanium plates with appropriate specifications for different cervical vertebrae in a cervical vertebral dome expansion laminoplasty.