bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Influence of Electronic Health Record Use on Collaboration Among Medical Specialties
Authors: Vos Janita F.J. , Boonstra Albert , Kooistra Arjen , Seelen Marc , Offenbeek Marjolein van 
Published: May 2020

Abstract
Background:One of the main objectives ofElectronic Health Records (EHRs) is to enhancecollaboration among healthcare professionals. However, our knowledge of how EHRs actually affect collaborative practices is limited. This study examines how an EHR facilitates and constrains collaborationin five outpatient clinics.Methods: We conducted an embedded case study at five multidisciplinary outpatient clinics of a hospital that had implemented organization-wide EHR. Data were collected through semi-structured interviews with representatives of medical specialties, administration, nursing, and management. Documents were then analyzed to contextualize these data. We examined the following six collaborative affordances of EHRs: (1) portability, (2) co-located access, (3) shared overviews, (4) mutual awareness, (5) messaging, and (6) orchestrating.Results:Our findings demonstratehow an EHRwill simultaneously bothfacilitate andconstrain collaborationamong specialties and disciplines. Affordances that were inscribed in the system for collaboration purposeswere not fully actualized in the focal hospital because:(a)The EHR helps health professionalscoordinate patient care on an informed basis at any time and in any placebut only allows asynchronouspatient record use.(b)The comprehensive patient file affords joint clinical decision-making based on shared data, but specialty- and discipline-specific user-interfaces constrain mutual understanding of that data. Moreover, not all relevant information can be easily shared across specialties and outside the hospital.(c)The reduced necessity forface-to-face communication saves time but is experienced as hindering collective responsibility for a smooth workflow.(d)The EHR affords registration at the source and full registration of activities through orders, but the heightened administrative burdenfor physicians and the strict authorization rules on inputting dataconstrainthe flexible, multidisciplinary collaboration.(e) While the EHR affords a complete overview, information overload occurs due to the parallel generation of individually owned notes and the high frequency of asynchronous communication through messages of varying clinical priority.Conclusions: For the optimal actualization of EHRs’collaborative affordancesin hospitals, coordinated use of these affordancesby health professionalsis a prerequisite.Suchcoordinated userequires organizational, technical, and behavioral adaptations. Suggestions for hospital-wide policies toenhance trust in both the EHR and in its coordinated usefor effective collaboration are offered.
Representativeness of Personality and Involvement Preferences in a Web-Based Survey on Healthcare Decision-Making
Authors: Birkeland Søren , Linkhorst Thea , Haakonsson Anders , Barry Michael John , Möller Sören 
Published: May 2020

Abstract
Background: Obtaining a sample that is representative of the group of interest is of utmost importance in questionnaire studies. In a survey using a state authorized web-portal for citizen communication with authorities, we wanted to investigate the view of adult men on patient involvement in health care decision-making regarding Prostate-Specific Antigen test for prostatic cancer. In this paper, we report on sample characteristics and representativeness of our sample in terms of personality and baseline involvement preferences.Methods: We compared personality profiles (BFI-10) and baseline healthcare decision-making preferences (CPS) in our sample (n = 6,756) to internationally available datasets. Pooled data from a) US, UK, Canada, Australia, and New Zealand (n = 1,512), b) Germany, Netherlands, Switzerland, and Belgium (n = 1,136), and c) Norway, Sweden, Finland, and Denmark (n = 1,313) were used for BFI-10 comparisons. Regarding CPS, we compared our sample with three previous datasets relating to decision-making in cancer (n = 425, 387, and 199).Results: Although statistically significant differences particularly appeared in large dataset comparisons, sample BFI-10 and CPS profiles mostly were within the range of those previously reported. Similarity was greatest in BFI-10 comparisons with group a) where no statistically significant difference could be established in factors ‘agreeableness’ and ‘neuroticism’ (p = .095 and .578, respectively).Conclusion: Despite some variation, our sample displays personality and baseline preference profiles that are generally similar to those described in previous international studies. For example, this was the case with the BFI-10 ‘agreeableness’ measure (incl. trust and fault-finding items), an important factor in healthcare decision-making.
A novel signature of two long non-coding RNAs in BRCA mutant ovarian cancer to predict prognosis and efficiency of chemotherapy
Authors: Pan Yinglian , Jia LiPing , Liu Yuzhu , Han Yiyu , Li Qian , Zou Qin , Zhang Zhongpei , huang jin , Deng Qingchun 
Published: May 2020

Abstract
Background: Ovarian cancer (OV) is the most common type of primary female reproductive cancer. BRCA1/2 gene is an important biomarker for evaluating the risk of OV, breast cancer and other related tumors and influences patient choice of individualized treatment. A powerful signature to predict OV prognosis and improve treatment personalization is urgently needed. This study aimed to identify a novel OV-related lncRNA prognostic biomarker.Methods: A Univariate Cox proportional-hazards and multivariate Cox regression analyses were used to identifying prognostic factors from The Cancer Genome Atlas (TCGA) database. The area under the curve (AUC) of the receiver operating characteristic (ROC) curve was assessed, and the sensitivity and specificity of the prediction model were determined.Results: The signature consisting of two long noncoding RNAs(lncRNAs), Z98885.2 and AC011601.1, was selected as a criterion for classifying patients into high and low-risk groups (median survival: 7.2 years vs. 2.3 years). The 3-year overall survival (OS) rates for the high- and low-risk groups were approximately 38% and 100%, respectively. Chemotherapy treatment survival rates indicated that high-risk groups had significantly shorter OS rates with adjuvant chemotherapy than the low-risk groups. The OS of 1-, 3- and 5- years were 100%, 40%, and 15% in the high-risk groups respectively. The survival rate of the high-risk group declined rapidly after two years of OA chemotherapy treatment. In addition, multivariate Cox regression associated with other traditional clinical factors showed that the 2-lncRNA model could be used as an independent OV prognostic factor. Analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) indicated that these signatures are pivotal to cancer development.Conclusion: In conclusion, Z98885.2 and AC011601.1 comprise a novel prognostic signature for OV patients with in BRCA1/2 mutations to predict prognosis and chemotherapy efficiency.
The Quality of Lymph Node Harvests in Extralevator Abdominoperineal Excisions
Authors: Liu Ben , Farquharson Ja’Quay 
Published: May 2020

Abstract
Background:Lymph node (LN) harvest in colorectal cancer resections is a well-recognised prognostic factor for disease staging and determining survival, particularly for node-negative (N0) diseases. Extralevator abdominoperineal excisions (ELAPE) aim to prevent “waisting” that occurs during conventional abdominoperineal resections (APR) for low rectal cancers, and reducing circumferential resection margin (CRM) infiltration rate. Our study investigates whether ELAPE may also improve the quality of LN harvests, addressing gaps in the literature.Methods:This retrospective observational study reviewed 2 sets of 30 consecutive APRs before and after the adoption of ELAPE in our unit. The primary outcomes are the total LN count and rates of meeting the standard of 12-minimum, particularly for those with node-negative disease. The secondary outcomes are the CRM involvement rates. Baseline characteristic including age, sex, laparoscopic or open surgery and neoadjuvant chemoradiotherapy were accounted for in our analyses.Results:Median LN counts were slightly higher in the ELAPE group (16.5 vs. 15). Specimens failing the minimum 12-LN requirements were almost significantly fewer in the ELAPE group (OR=0.456). Among node-negative rectal cancers, significantly fewer resections failed the 12-LN standard in the ELAPE group than APR group (OR=0.211, p=0.044). ELAPE led to a near-significant decrease in CRM involvement (OR=0.365). These improvements were persistently observed after taking into account baselines and potential confounders in regression analyses. ConclusionELAPE provides higher quality of LN harvests that meet the 12-minimal requirements than conventional APR, particularly in node-negative rectal cancers. The superiority is independent of potential confounding factors and may implicate better clinical outcomes.
Inflammation is Correlated with Abnormal Functional Connectivity in Unmedicated Bipolar Depression: A Resting State FMRI Study
Authors: Tang Guixian , Chen Pan , Chen Guanmao , Zhong Shuming , Gong Jiaying , Zhong Hui , Ye Tao , Chen Feng , Wang Jurong , Luo Zhenye , Qi Zhangzhang , Jia Yanbin , Wang Ying , Huang Li 
Published: May 2020

Abstract
Objectives Inflammation might play a role in bipolar disorder (BD), but it remains unclear the relationship between inflammation and brain structural and functional abnormalities in patients with BD. In this study, we focused on the alterations of functional connectivity (FC), peripheral pro-inflammatory cytokines and their correlations to investigate the role of inflammation in FC in BD depression.Methods In this study, 42 unmedicated patients with BD II depression and 62 healthy controls (HCs) were enrolled. Resting-state-functional magnetic resonance imaging (rs-fMRI) was performed in all participants and independent component analysis (ICA) was used. Serum levels of Interleukin-6 (IL-6) and Interleukin-8 (IL-8) were measured in all participants. Correlation between FC values and IL-6 and IL-8 levels in BD was calculated.Results Compared with the HCs, BD II patients showed decreased FC in the left orbitofrontal cortex (OFC) implicating the limbic network and the right precentral gyrus implicating the somatomotor network (SMN). BD II showed increased IL-6 (P = 0.039), IL-8 (P = 0.002) levels. Moreover, abnormal FC in the right precentral gyrus were inversely correlated with the IL-8 (r=-0.458, P = 0.004) levels in BD II. No significant correlation was found between FC in the left OFC and cytokines levels.Conclusions Our findings that serum IL-8 levels is associated with impaired FC in the right precentral gyrus in BD II patients suggest that inflammation might play a crucial role in brain functional abnormalities in BD.
SKP1 promotes YAP-mediated colorectal cancer stemness via suppressing RASSF1
Authors: Tian Cong , Lang Tingyuan , Qiu Jiangfeng , Han Kun , Zhou Lei , Min Daliu , Zhang Zhiqi , Qi Dachuan 
Published: May 2020

Abstract
Background: Cancer stem cells have been recognized as an important drug target, however, the mechanisms underlying the maintenance of cancer stem cells have not been fully understood. SKP1 is a traditional drug target for cancer therapy, while, whether SKP1 could be a target for eradicating cancer stem cells remains elusive.Methods: Human colorectal cancer cell lines HCT-116 and HT-29 and primary human colorectal cancer cells were used in this study. Gene manipulation was performed by lentivirus system. The mRNA and protein levels were examined by qRT-PCR and western blot, respectively. Sphere formation and transwell assay were employed for examination of sphere-forming and migration capacities. The tumorigenicity was examined by xenograft model. The transcriptional activities of the promoters were examined by luciferase reporter assay. Co-immunoprecipitation assay was used to test protein-protein interaction. The relationship between gene expression and survival was analyzed by Kaplan-meier analysis. The correlation between two genes was analyzed by Spearman analysis. Data are represented as mean ± s.d. and the significance was determined by Student’s t-test.Results: SKP1 is upregulated in colorectal cancer stem cells and predicts poor prognosis of colon cancer patients. Overexpression of SKP1 promotes the sphere-forming and migration capacities of colorectal cancer stem cells, and upregulates the expression of cancer stem cell markers. In contrast, SKP1 depletion produces the opposite effects. SKP1 strengthens YAP activity and knockdown of YAP abolished the effect of SKP1 on the stemness of colorectal cancer cells. SKP1 suppresses RASSF1 at both mRNA and protein levels and overexpression of RASSF1 abolished the effect of SKP1.Conclusion: In summary, our results demonstrated that SKP1 suppresses RASSF1 at both mRNA and protein level, attenuates Hippo signaling, activates YAP, and thereby promoting the stemness of colorectal cancer stem cells. Our works thus revealed a novel underlying mechanism of colorectal cancer stem cell maintenance and suggested a novel drug target for eradicating colorectal cancer stem cells.
Downregulation of long non-coding RNA SNHG7 protects against inflammation and apoptosis in Parkinson's disease model by targeting miR-425-5p/TRAF5/NF-κB axis
Authors: Yu Shan , Liu Xueshibojie , Yu Duo , E Changyong , Yang Jinghui 
Published: May 2020

Abstract
Background

Accumulated evidence has established that long non-coding RNA (lncRNA) is involved in the progress of Parkinson's disease (PD). SNHG7, a novel lncRNA, has been found to play a key role in tumorigenesis. However, the SNHG7 expression and its functional effects on PD remain uncharted.
Methods

RT-PCR was used to detect the expression of SNHG7, miR-425-5p and inflammatory cytokines in the plasma of PD patients and the healthy controls. Rotenone (Rot) was adopted to construct PD models in SD rats and SH-SY5Y cells, respectively. Gain- and loss- of functions of SNHG7 or miR-425-5p were conducted. The expression levels of Caspase3, tyrosine hydroxylase (TH), Iba1 in SD rat striatum was measured via immunohistochemistry and Western blot. Additionally, the expressions of inflammatory cytokines (IL-1β, IL-6, TNF-α) and oxidative stress factors (MDA, SOD, GSH-PX) in the brain tissues were examined using RT-PCR and ELISA. Moreover, the protein levels of TRAF5, I-κB, NF-κB, HO-1, Nrf2 were detected via Western blot. Bioinformatics was applied to predict the targeting relationship between SNHG7, miR-425-5p and TRAF5. Dual luciferase activity assay and RNA immunoprecipitation (RIP) assays were carried out to verify their interactions.
Results

SNHG7 was found up-regulated in PD patients while miR-425-5p expression was down-regulated (compared to healthy donors). Meanwhile, SNHG7 level was positively correlated with the level of inflammatory cytokines in PD patients. Functional experiments confirmed that SNHG7 downregulation or miR-425-5p overexpression attenuated neuronal apoptosis in the Rot-mediated PD model, TH-positive cell loss and microglia activation by mitigating inflammation and oxidative stress. Mechanistically, SNHG7 served as a competitive endogenous RNA (ceRNA) by sponging miR-425-5p and promoted TRAF5 mediated inflammation and oxidative stress.
Conclusion

Inhibition of SNHG7 ameliorated neuronal apoptosis in PD through relieving miR-425-5p/TRAF5/NF-κB signaling pathway modulated inflammation and oxidative stress.
Experiences with Health Care and Health-Related Quality of Life of Patients with Hematological Malignancies in Mexico
Authors: Doubova Svetlana Vladislavovna , Terreros-Muñoz Eduardo , Delgado-Lòpez Nancy , Montaño-Figueroa Efreen Horacio , Infante-Castañeda Claudia , Cuevas Ricardo Perez 
Published: May 2020

Abstract
Introduction: In Mexico patients with hematologic malignancies (HMs) are characterized by high risk, advanced stages at diagnosis and a low cure rate; yet the information on their experiences with healthcare and health-related quality of life (HRQL) is scarce. We aimed to evaluate experiences with healthcare and HRQL of patients with HMs.Methods: We conducted a cross-sectional survey in two oncology hospitals in Mexico City. The study included outpatient cancer patients aged ≥ 18 years with a diagnosis of leukemia, lymphoma, or multiple myeloma. We used a patient-centered quality of cancer care questionnaire to assess experiences with care and the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30) to measure HRQL. We performed a multiple linear regression to evaluate the association between patient experiences and the QLQ-C30 summary score.Results: Of the 515 participating HM patients, 46.6% had lymphoma, 34% leukemia, and 19.4% multiple myeloma; 70.9% were at advanced stages or at high risk; 15.1% had anxiety and 12.8% had depression. Over one third (35.9%) received clear information, 28.5% timely care, 20.6% information for treatment decision-making, 23.7% care that addressed their biopsychological needs, and 31% respectful and coordinated care. The mean QLQ-C30 summary score was 71.9 points. Timely care, clarity of the information, and care that addresses biopsychological needs were associated with higher HRQL.Conclusions: Healthcare providers are encouraged to measure and address the experiences with healthcare of HM patients to improve the quality of patient-centered cancer care and increase the likelihood of better HRQL.
The clinical and prognostic significance of HER2 and Ki67 expression in 2062 Chinese patients with resectable gastric cancer: a retrospective study
Authors: Wang Wenfan , Chen Zhiqiang , Zhang Qinghua , You Yanjie , Ma Ailing , Wang Guanhua , Cao Juan , Zhao Dan , Zhang Xiaoxu , Zhang Qian , Wu Jing , Ruan Jigang , Yang Wenjun 
Published: May 2020

Abstract
Background

The purpose of this study was to assess the prognostic value of the expression of human epidermal growth factor receptor 2 (HER2), Ki67, and their combination levels in the prognosis of Chinese patients with resectable gastric cancer (GC).
Methods

A total of 2062 Chinese GC patients were recruited with HER2 and Ki67 expression being evaluated using immunohistochemistry. Patients were divided into four groups according to HER2 and Ki67 expression. The distributions between HER2 and Ki67 expression levels and clinicopathological characteristics were compared using the Chi-square test. The relationship between HER2 and Ki67 expression level and overall survival were evaluated with the univariate and multivariate Cox regression analysis.
Results

There was no statistical differences between the overall survival (OS) rate and the expression level of HER2 (P = 0.748) or Ki67 (P = 0.063), but there were significant relationships between the OS rates and the combining expression levels of HER2/Ki67 (P < 0.05). Further, Ki67, sex, T stage, N stage, TNM stage, and adjuvant chemotherapy were significant and independent risk factors for GC survival (P < 0.05).
Conclusions

Our study illustrated that Ki67, but not HER2 acted as an independent prognostic factor in Chinese resectable GC patients. The evaluation of the combining expression levels of HER2/Ki67 may be more useful to assess patient prognosis with resectable GC.
Asthma susceptible genes in children: A meta-analysis
Authors: Ruan Zhen , Shi Zhaoling , Zhang Guocheng , Kou Jiushe , Ding Hui 
Published: May 2020

Abstract
Background: During the last decade, a number of studies have evaluated the potential association between some genetic polymorphisms and childhood asthma risk, however, the results of published studies appeared conflicts. The aim of the present study was to investigate association between genetic polymorphisms and pediatric asthma. Methods: Relevant studies were searched in PubMed, Embase, Web of Science, CNKI (China National Knowledge Infrastructure), Wanfang and Weipu database. Pooled odds ratios (OR) with 95% confidence interval (CI) were calculated to access the strength of the associations. Results: 45 case-control studies were finally included in this meta-analysis. Eighteen SNPs were identified. Ten polymorphisms were found to be associated with asthma risk: IL-13 +2044G/A, IL-4 -590C/T, ADAM33 F+1, ADAM33 T2, ADAM33 T1, ADAM33 ST+4, ORMDL3 rs7216389, VDR ApaⅠ, VDR FokⅠ, VDR BsmⅠ. After subgroup analysis, IL-13 +2044G/A, ADAM33 T2, ADAM33 T1, VDR ApaⅠ, VDR BsmⅠ polymorphisms were associated with increased risk of asthma among Asian populations. IL-4 -590C/T polymorphisms may be related to asthma risk in Chinese children.Conclusions: This meta-analysis found that IL-13 +2044G/A, IL-4 -590C/T, ADAM33 F+1, ADAM33 T2, ADAM33 T1, ADAM33 ST+4, ORMDL3 rs7216389, VDR ApaⅠ, VDR FokⅠ and VDR BsmⅠ polymorphisms might be risk factors for childhood asthma. Further study with large population and more ethnicities is needed to estimate the associations.
Alpha-1 antitrypsin deficient individuals have circulating extracellular vesicles with profibrogenic cargo
Authors: Khodayari Nazli , Oshins Regina , Holliday Shannon , Clark Virginia , Xiao Qiang , Marek George , Mehrad Borna , Brantly Mark 
Published: May 2020

Abstract
Background: Alpha-1 antitrypsin deficiency (AATD)-mediated liver disease is a toxic “gain-of- function” inflammation in the liver associated with intracellular retention of mutant alpha-1 antitrypsin. The clinical presentation of the disease includes fibrosis, cirrhosis and liver failure. However, the pathogenic mechanism of AATD-mediated liver disease is not well understood. Here, we investigated the role of plasma extracellular vesicles (EVs) in progression of AATD- mediated liver disease. Methods: EVs were isolated from plasma of AATD individuals with liver disease and healthy controls. Their cytokines and miRNA content were examined by multiplex assay and small RNA sequencing. The bioactivity of EVs was assessed by qPCR, western blot analysis and immunofluorescent experiments using human hepatic stellate cells (HSCs) treated with EVs isolated from control or AATD plasma samples. Results: We have found that AATD individuals have a distinct population of EVs with pathological cytokine and miRNA contents. When HSCs were cultured with AATD plasma derived-EVs, the expression of genes related to the development of fibrosis were significantly amplified compared to those treated with healthy control plasma EVs.Conclusion: AATD individuals have a distinct population of EVs with abnormal cytokine and miRNA contents and the capacity to activate HSCs and mediate fibrosis. Better understanding of the components which cause liver inflammation and fibrogenesis, leading to further liver injury, has the potential to lead to the development of new treatments or preventive strategies to prevent AATD-mediated liver disease.