bluuebunny/crossref_metadata_2025_split huggingface dataset:
The views and experiences of bisexual people regarding their psychosocial support needs: a qualitative evidence synthesis
Authors: McCann Edward , Brown Michael J. , Taylor Julia 
Published: August 2020

Accessible SummaryWhat is known on the subject?

Bisexual people experience significantly poorer mental health and higher rates of self‐harm and suicidality than their gay, lesbian or heterosexual counterparts.

Although little is understood about why these disparities exist, bisexual people have been found to have unique life experiences that separate them from other sexual minority groups.

Despite these health disparities and unique experiences, in research and in clinical settings, bisexual people are often incorporated under the LGBTQI+ umbrella and their needs are not seen as being different to those of other sub‐groups.

What the paper adds to existing knowledge?

This paper brings together the findings of the small but growing body of literature reporting on the psychosocial support needs of bisexual people.

The findings reported on herein provide novel insights, collated and synthesized that will prove valuable to policy makers, service providers and researchers.

What are the implications for practice?

This paper clearly illustrates that bisexual people experience significant barriers to accessing the support services they need and that current competent service provision for this population is severely limited.

The findings presented in this paper shed light on the unique psychosocial needs of bisexual people and highlight the need for practice change to reduce barriers to service access and ensure inclusive and culturally competent care for this community.

AbstractIntroductionDespite consistent evidence of poor mental health among what is a relatively large population group, research examining bisexual mental health remains sparse.AimTo identify the psychosocial needs of people who are bisexual and establish factors that may support or inhibit access to appropriate psychosocial interventions and supports.MethodA qualitative evidence syntheses of the empirical evidence.ResultsA total of 15 papers were included in the review. The PRISMA process was used. Following data analysis, four main themes emerged that were (a) experiences of being bisexual, (b) mental health experiences and concerns, (c) service access and responses and (iv) communities and supports.DiscussionBisexual people have unique and specific psychosocial support needs that relate to, but are also different from, the needs of the broader LGBTIQ+ community. This study provides valuable insights into how future policy, practice, education and training and research can better address the needs of this highly vulnerable group.Implications for PracticeThis study highlights the psychosocial complexities associated with bisexuality and provides evidence for the need for improvement in current support services to ensure inclusivity and culturally competent care.
A deletion of IDUA exon 10 in a family of Golden Retriever dogs with an attenuated form of mucopolysaccharidosis type I
Authors: Faller Kiterie M. E. , Ridyard Alison E. , Gutierrez‐Quintana Rodrigo , Rupp Angie , Kun‐Rodrigues Celia , Orme Tatiana , Tylee Karen L. , Church Heather J. , Guerreiro Rita , Bras Jose 
Published: August 2020

AbstractBackgroundMucopolysaccharidosis type I (MPS‐I) is a lysosomal storage disorder caused by a deficiency of the enzyme α‐l‐iduronidase, leading to accumulation of undegraded dermatan and heparan sulfates in the cells and secondary multiorgan dysfunction. In humans, depending upon the nature of the underlying mutation(s) in the IDUA gene, the condition presents with a spectrum of clinical severity.ObjectivesTo characterize the clinical and biochemical phenotypes, and the genotype of a family of Golden Retriever dogs.AnimalsTwo affected siblings and 11 related dogs.MethodsFamily study. Urine metabolic screening and leucocyte lysosomal enzyme activity assays were performed for biochemical characterization. Whole genome sequencing was used to identify the causal mutation.ResultsThe clinical signs shown by the proband resemble the human attenuated form of the disease, with a dysmorphic appearance, musculoskeletal, ocular and cardiac defects, and survival to adulthood. Urinary metabolic studies identified high levels of dermatan sulfate, heparan sulfate, and heparin. Lysosomal enzyme activities demonstrated deficiency in α‐l‐iduronidase activity in leucocytes. Genome sequencing revealed a novel homozygous deletion of 287 bp resulting in full deletion of exon 10 of the IDUA gene (NC_006585.3(NM_001313883.1):c.1400‐76_1521+89del). Treatment with pentosan polyphosphate improved the clinical signs until euthanasia at 4.5 years.Conclusion and Clinical ImportanceAnalysis of the genotype/phenotype correlation in this dog family suggests that dogs with MPS‐I could have a less severe phenotype than humans, even in the presence of severe mutations. Treatment with pentosan polyphosphate should be considered in dogs with MPS‐I.
Reef microhabitats mediate fish feeding intensity and agonistic interactions at Príncipe Island Biosphere Reserve, Tropical Eastern Atlantic
Authors: Canterle Angela Marina , Nunes Lucas Teixeira , Fontoura Luisa , Maia Hugulay Albuquerque , Floeter Sergio Ricardo 
Published: August 2020

AbstractThe benthic assemblage of reefs provides an important resource of food and habitat for reef fishes. However, how benthic composition mediates reef fishes' biotic interactions at isolated environments such as oceanic islands remains largely unknown. Here, we aimed to investigate the role of four different reef microhabitats over biological interactions of fishes in an understudied oceanic island, Príncipe Island. For that, we recorded a total of 46 Underwater Remote Videos (RUVs) to document benthic composition and fishes' trophic and agonistic interactions. We used benthic cover estimates to group the samples into four microhabitats (dominated by epilithic algal matrix [EAM], sand/rock, corals and sponges), then quantified fishes' trophic and agonistic interactions in each microhabitat. All microhabitats presented a different structure of trophic and agonistic interactions of the fish assemblage. Feeding pressure (FP) and agonistic interactions were higher on the EAM microhabitat and lower in coral microhabitat. Herbivores were the main responsible group for the FP in all microhabitats. Territorial damselfishes used microhabitats differently for both trophic and agonistic interactions. We demonstrated that reef fish diversity and intensity of biotic interactions varied according the spatial distribution of benthic resources, which suggests that benthic composition plays an important role on structuring biological interactions at isolated reef systems.
Tunnel condition assessment via cloud model‐based random forests and self‐training approach
Authors: Zhu Mengqi , Zhu Hehua , Guo Feng , Chen Xueqin , Ju J. Woody 
Published: August 2020

AbstractTo proactively assess the losses caused by the deterioration of metro tunnels during the operational period, a new method, the cloud model‐based random forests (CRFs), is proposed to discuss the inconsistencies induced by mapping the monitoring data into the health rate of the metro tunnels. On top of the CRF, a self‐training framework is introduced to improve the predictive accuracy and the stability of the CRF by adding more unlabeled data. The main contribution of this paper is proposing a novel CRF model along with a semisupervised approach to overcome the inapplicability of the random forests algorithm in an inconsistent small database, and to reduce the substantial time and cost for expert annotation. The results indicate that (1) the proposed CRF achieves higher accuracy and stability than random forests in predictions; (2) the CRF outperforms the other state‐of‐the‐art methods even in a small database; (3) the self‐training improved CRF keeps highly precise when the ratio of labeled to unlabeled data is no less than 1:11.4. In this study, the suggested ratio of labeled and unlabeled data is no lower than 1:5.7 to reduce the risk of wrongly forecasting a seriously damaged tunnel section as a slightly damaged tunnel section.
Usefulness of an isoproterenol infusion to differentiate a left atrial appendage thrombus in a patient with nonvalvular atrial fibrillation
Authors: Enomoto Yoshinari , Hara Hidehiko , Makino Kenji , Nakamura Keijiro , Sugi Kaoru , Moroi Masao , Nakamura Masato 
Published: August 2020

AbstractA 78‐year‐old male with a history of a cardiac embolic stroke due to persistent AF and cerebral bleeding (CHADS2 score 4, HAS‐BLED score 4) was referred to our hospital to implant a left atrial appendage (LAA) closure (LAAC) device. A trans esophageal echocardiography was performed and a high echoic lesion that was difficult to differentiate the spontaneous echo contrast or thrombus was found in the LAA cavity. After isoproterenol infusion, a high echoic lesion disappeared and we confirmed that it was not an LAA thrombus. Successful LAAC device implantation was performed without any thromboembolic events.
Pediatric trichodysplasia spinulosa: A report of 2 cases and review of the literature
Authors: Rusk Amanda M. , Crute Meghan M. , Goebel Jens , Schecter Marc G. , Gupta Anita , Schwentker Ann R. , Sheth Anita P. , Lucky Anne W. 
Published: August 2020

AbstractTrichodysplasia spinulosa is a rare disorder caused by the ubiquitous trichodysplasia spinulosa‐associated polyomavirus (TSPyV) and characterized clinically by predominately centrofacial, but often generalized, folliculocentric papules with protuberant keratinaceous spines. Although seroprevalence reaches up to 70% in adult populations, TSPyV causes clinical manifestations in a small percentage of patients who are immunosuppressed. Diagnosis can be made using typical clinical and histologic features, SV40T antibody immunostaining, and PCR of various tissues including the keratinaceous spine, skin, serum, urine, and CSF. Various topical and systemic medications have demonstrated variable success. Decreasing or discontinuing immunosuppression has also been shown to improve or alleviate clinical manifestations.
Mendelian randomisation approaches to the study of prenatal exposures: A systematic review
Authors: Diemer Elizabeth W. , Labrecque Jeremy A. , Neumann Alexander , Tiemeier Henning , Swanson Sonja A. 
Published: August 2020

AbstractBackgroundMendelian randomisation (MR) designs apply instrumental variable techniques using genetic variants to study causal effects. MR is increasingly used to evaluate the role of maternal exposures during pregnancy on offspring health.ObjectivesWe review the application of MR to prenatal exposures and describe reporting of methodologic challenges in this area.Data sourcesWe searched PubMed, EMBASE, Medline Ovid, Cochrane Central, Web of Science, and Google Scholar.Study selection and data extractionEligible studies met the following criteria: (a) a maternal pregnancy exposure; (b) an outcome assessed in offspring of the pregnancy; and (c) a genetic variant or score proposed as an instrument or proxy for an exposure.SynthesisWe quantified the frequency of reporting of MR conditions stated, techniques used to examine assumption plausibility, and reported limitations.ResultsForty‐three eligible studies were identified. When discussing challenges or limitations, the most common issues described were known potential biases in the broader MR literature, including population stratification (n = 29), weak instrument bias (n = 18), and certain types of pleiotropy (n = 30). Of 22 studies presenting point estimates for the effect of exposure, four defined their causal estimand. Twenty‐four studies discussed issues unique to prenatal MR, including selection on pregnancy (n = 1) and pleiotropy via postnatal exposure (n = 10) or offspring genotype (n = 20).ConclusionsPrenatal MR studies frequently discuss issues that affect all MR studies, but rarely discuss problems specific to the prenatal context, including selection on pregnancy and effects of postnatal exposure. Future prenatal MR studies should report and attempt to falsify their assumptions, with particular attention to issues specific to prenatal MR. Further research is needed to evaluate the impacts of biases unique to prenatal MR in practice.
Implications of ram sperm rheotaxis analysed by microfluidics for fertility
Authors: Abdel‐Ghani Mohammed A. , El‐sherry Timor , Mahmoud Gamal , Nagano Masashi 
Published: August 2020

AbstractRheotaxis of sperm using a microfluidic device was explored in human, mice and bull. However, the rheotaxis of ram sperm and its role in fertility are unknown. Herein, we described the sperm rheotaxis in ram using microfluidic devices and focused on rheotaxis as potential markers of in vivo fertility. Computer‐assisted sperm analysis (CASA) with controlled flow velocity was used to explore the kinematic parameters of sperm, total motility and positive rheotaxis (PR). The percentage of PR was defined as the number of PR sperm cells over the number of motile sperm cells. Then, according to the percentage of PR sperm, rams were classified into two groups; sperm with ≥40% PR and <40% PR, although the two ram groups showed similar total motility and kinematic values of sperm evaluated by CASA (p > .05). Two groups of rams mated one hundred thirty ewes naturally (10 ewes/ram). In the results, the pregnancy rate was higher in ≥40% PR (94.4%) than in <40% PR (42.5%, p < .05) after natural mating. Besides, the pregnancy loss was higher in <40% PR (33.3%) than in >40% PR group (8.1%, p < .05). In conclusion, the PR examination in semen can contribute to evaluate the reproductive performance of ram that will provide valuable insights into the semen evaluation.
Remote monitoring system as a tool for calving management in Mediterranean Buffalo heifers (Bubalus bubalis)
Authors: Rossi Emanuela , Ferri Nicola , Crociati Martina , Monaci Maurizio , Stradaioli Giuseppe , Sylla Lakamy 
Published: August 2020

AbstractBuffalo breeding is common in Southern Italy. Dystocia compromises dam's and newborn health and welfare. Difficult parturition could be solved through prompt calving assistance, even if the identification of the beginning of delivery is challenging. Herein, we aimed to evaluate a remote calving alarm system in 15 Mediterranean buffalo heifers. An intravaginal probe was placed close to the external cervical os once premonitory signs of delivery were observed. No vaginal discharge nor signs of discomfort were notified in the days following the insertion of the probe. Heifers calved from 48 to 72 hr after the alarm was activated. The system correctly warned the farm personnel at the beginning of stage II of parturition, except for 2 cases. In the former, the intravaginal probe was expelled but the poor carrier network coverage negatively affected phone's signal quality; in the latter, recurrent vaginal prolapse was responsible for non‐retention of the probe. Overall median expulsive phase was 68 ± 8 min, while the expulsion of a female calf took 54 ± 22.0 min and 90 ± 34.0 min in males, with significant difference (p =.02). Deliveries were homogeneously distributed across a 24‐hr interval. No retention of foetal membranes nor metritis was identified at postpartum clinical examination. The calving alarm system used in this work was well tolerated in buffalo heifers. The introduction of smart technology in buffalo farming could contribute to the overall farm net return by reducing calf losses, especially for calves born from sexed‐sorted semen, and by increasing animal welfare through quick resolution of dystocia. Further studies will be necessary to evaluate the net return in buffalo farms which will implement a remote calving alarm system on a wider population.
CropPAL for discovering divergence in protein subcellular location in crops to support strategies for molecular crop breeding
Authors: Hooper Cornelia M. , Castleden Ian R. , Aryamanesh Nader , Black Kylie , Grasso Sally V. , Millar A. Harvey 
Published: August 2020

SUMMARYAgriculture faces increasing demand for yield, higher plant‐derived protein content and diversity while facing pressure to achieve sustainability. Although the genomes of many of the important crops have been sequenced, the subcellular locations of most of the encoded proteins remain unknown or are only predicted. Protein subcellular location is crucial in determining protein function and accumulation patterns in plants, and is critical for targeted improvements in yield and resilience. Integrating location data from over 800 studies for 12 major crop species into the cropPAL2020 data collection showed that while >80% of proteins in most species are not localised by experimental data, combining species data or integrating predictions can help bridge gaps at similar accuracy. The collation and integration of over 61 505 experimental localisations and more than 6 million predictions showed that the relative sizes of the protein catalogues located in different subcellular compartments are comparable between crops and Arabidopsis. A comprehensive cross‐species comparison showed that between 50% and 80% of the subcellulomes are conserved across species and that conservation only depends to some degree on the phylogenetic relationship of the species. Protein subcellular locations in major biosynthesis pathways are more often conserved than in metabolic pathways. Underlying this conservation is a clear potential for subcellular diversity in protein location between species by means of gene duplication and alternative splicing. Our cropPAL data set and search platform (https://crop‐pal.org) provide a comprehensive subcellular proteomics resource to drive compartmentation‐based approaches for improving yield, protein composition and resilience in future crop varieties.
Seizure activity following atracurium continuous rate infusion in three mechanically ventilated juvenile dogs
Authors: Donaldson Rebekah E , Cortellini Stefano , Humm Karen 
Published: August 2020

AbstractObjectiveTo describe patient recovery in dogs that had undergone mechanical ventilation (MV) and received atracurium as a continuous rate infusion (CRI).Case series summaryThree dogs were treated with atracurium CRI while mechanically ventilated and were successfully weaned. All were pediatric patients (2‐3 months old) ventilated due to respiratory fatigue and hypoxemia. All dogs experienced seizure activity in the initial 24 h following extubation and were treated with various anticonvulsant protocols; no patients experienced documented seizures after 48 h of discontinuation from MV.New or unique information providedAll three successfully weaned dogs that had received an atracurium CRI experienced new onset seizures in their recovery from MV. Atracurium use in long‐term MV has not been described previously, and seizures in dogs following atracurium administration has not been described in the clinical setting. Although causation cannot be proven in this case series, atracurium use should be carefully considered and be monitored appropriately when used for a prolonged duration.
Effect of sedation or general anesthesia on elbow goniometry and thoracic limb circumference measurements in dogs with naturally occurring elbow osteoarthritis
Authors: Clarke Emily , Aulakh Karanvir S. , Hudson Caleb , Barnes Katherine , Gines J. Alberto , Liu Chin‐Chi , Aulakh Harmeet K. 
Published: August 2020

AbstractObjectiveTo evaluate the effect of sedation or general anesthesia (GA) on elbow goniometry and thoracic limb circumference (TLC) measurements in dogs with elbow osteoarthritis (OA).Study designProspective study.AnimalsTwenty‐four client‐owned dogs with radiographically confirmed elbow OA.MethodsElbow goniometry and TLC measurements were made before and after either sedation or GA by using a hand‐held goniometer and spring tension measuring tape, respectively. Observers were not allowed to review their pre‐sedation or pre‐GA measurements at the time of obtaining measurements on dogs under sedation or GA. Mixed analysis of variance models were used to compare elbow goniometry and TLC measurements before and after sedation or GA.ResultsEleven and thirteen dogs were included in the sedation and GA groups, respectively. Mean elbow flexion decreased by 5° and 3° and mean elbow extension increased by 6° and 2° under sedation and GA, respectively. Total range of motion increased by 11° under sedation and by 5° under GA. Each of these changes was statistically significant (P < .05) except elbow extension under GA (P = .129). Sedation and GA did not influence TLC measurements (P > .05).ConclusionSedation or GA led to slight and similar increase in elbow flexion and extension but did not influence TLC measurements in dogs with elbow OA.Clinical significanceSedation or GA can cause slight alterations to goniometric measurements in canine elbows with OA. The protocols used in this study for sedation and GA seem interchangeably acceptable for goniometry and TLC measurements in dogs with elbow OA.
Obesity susceptible novel DNA methylation marker on regulatory region of inflammation gene: results from the Korea Epigenome Study (KES)
Authors: Koh In-Uk , Choi Nak-Hyeon , Lee Kibaick , Yu Ho-Yeong , Yun Jun Ho , Kong Jin-Hwa , Kim Hyo Jin , Lee Song , Kim Song Cheol , Kim Bong-Jo , Moon Sanghoon 
Published: August 2020

IntroductionObesity is growing global health concern and highly associated with increased risk of metabolic diseases including type 2 diabetes. We aimed to discover new differential DNA methylation patterns predisposing obesity and prioritize surrogate epigenetic markers in Koreans.Research design and methodsWe performed multistage epigenome-wide analyses to identify differentially expressed CpGs in obesity using the Illumina HumanMethylationEPIC array (EPIC). Forty-eight CpGs showed significant differences across three phases: 902 whole blood DNAs from two cohorts (phase 1: n=450, phase 2: n=377) and a hospital-based sample (phase 3: n=75). Samples from phase III participants were used to examine whether the 48 CpGs are significant in the fat tissue and influenced gene expression. Furthermore, we investigated the epigenetic effect of CpG loci in childhood obesity (n=94).ResultsSeven of the 48 CpGs exhibited similar changes in the fat tissue along with gene expression changes. In particular, hypomethylated CpG (cg13424229) on the GATA1 transcription factor cluster of CPA3 promoter was related to its increased gene expression and showed consistent effect in childhood obesity. Interestingly, subsequent analysis using RNA sequencing data from 21 preadipocytes and 26 adipocytes suggested CPA3 as a potential obesity-related gene. Moreover, expression patterns from RNA sequencing and public Gene Expression Omnibus showed the correlation between CPA3 and type 2 diabetes (T2D) and asthma.ConclusionsOur finding prioritizes influential genes in obesity and provides new evidence for the role of CPA3 linking obesity, T2D, and asthma.