bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circadian expression of Glycoprotein 2 (Gp2) gene is controlled by a molecular clock in mouse Peyer's patches
Authors: Kusunose Naoki , Tsuruta Akito , Hamamura Kengo , Tsurudome Yuya , Yoshida Yuya , Akamine Takahiro , Matsunaga Naoya , Koyanagi Satoru , Ohdo Shigehiro 
Published: February 2020

AbstractThe expression levels of many cell‐surface proteins vary with the time of day. Glycoprotein 2 (Gp2), specifically expressed on the apical surface of M cells in Peyer's patches, functions as a transcytotic receptor for mucosal antigens. We report that cAMP response element‐binding protein (CREB) regulates the transcription of the Gp2 gene, thereby generating the circadian change in its expression in mouse Peyer's patches. The transcytotic receptor activity of Gp2 was increased during the dark phase when the Gp2 protein abundance increased. Rhythmic expression of clock gene mRNA was observed in mouse Peyer's patches, and expression levels of Gp2 mRNA also exhibited circadian oscillation, with peak levels during the early dark phase. The promoter region of the mouse Gp2 gene contains several cAMP response elements (CREs). Chromatin immunoprecipitation assays revealed that CREB bound to the CREs in the Gp2 gene in Peyer's patches. Forskolin, which promotes CREB phosphorylation, increased the transcription of the Gp2 gene in Peyer's patches. As phosphorylation of CREB protein was increased when Gp2 gene transcription was activated, CREB may regulate the rhythmic expression of Gp2 mRNA in Peyer's patches. These findings suggest that intestinal immunity is controlled by the circadian clock system.
Paying back student loans: Demographic, human capital and other correlates of default and repayment difficulty
Authors: Pizarro Milian Roger , Zarifa David , Seward Brad 
Published: February 2020

AbstractGovernment‐sponsored student loans have emerged over the decades as a primary method of financing post‐secondary education across most North American jurisdictions. Despite this, the empirical literature examining the correlates of repayment difficulty and default in Canada has remained stagnant in recent years. This study taps into an underutilised data source—the 2013 National Graduates Survey—to examine the relationship between demographics, human capital, borrowing behaviour and other known predictors and repayment difficulty. Our logistic regression models demonstrate that disability status, geographic region and borrowing behaviour are correlated with loan default and repayment difficulty, while failing to verify the existence of other demographic effects routinely found in the existing literature. We discuss the implications of these findings, along with multiple avenues for further empirical work on this topic within Canada.
MICA‐129 Met/Val polymorphism could be a genetic biomarker for Familial Breast Cancer in the Tunisian population
Authors: Ouni Nesrine , Ben Chaaben Arij , Ayari Fayza , Douik Hayet , Guizani Imen , Benammar‐Elgaaied Amel , Guemira Fethi , Tamouza Ryad 
Published: February 2020

AbstractIdentification of candidate genes associated with susceptibility of breast cancer can have a significant impact at a cancer management national healthcare systems level, making genetic testing more affordable and cost‐effective. We have previously shown that the major histocompatibility complex class I‐related chain A (MICA) was related to breast cancer and plays an important role in modulating immune response mechanisms through NKG2D receptor activation. Compared to our previous study, in this work, we recruited a new cohort composed of 354 unrelated Tunisian women affected by breast cancer and 380 age‐matched women as controls, all genotyped for MICA‐129 Met/Val (rs 1051792). Subsequently, we exanimated the distribution of this polymorphism in ten families. As a result, an association was found between the Val allele and Val/Val genotype and the risk of breast cancer (p = 2.5 × 10–15; OR = 2.40; p = 6.5 × 10–13; OR = 3.03, respectively). Stratified analysis with age and family history of cancer revealed an association between the Val/Val genotype and younger patients <40 years (p = .003; OR = 2.03). Among those patients having a family history of cancer, 68% had a Val/Val genotype (p = .02; OR = 1.82). In the family study, an analyse of pedigrees revealed that the majority of families showed the development of breast cancer at a young age. Moreover, all patients diagnosed with early‐onset breast cancer had a Val/Val genotype. Our results lead us to propose that this polymorphism may be an inherited genetic biomarker contributing to an increased breast cancer risk in Tunisian women.
Added sugar and trans fatty acid intake and sedentary behavior were associated with excess total‐body and central adiposity in children and adolescents with congenital heart disease
Authors: Honicky M. , Cardoso S. M. , de Lima L. R. A. , Ozcariz S. G. I. , Vieira F. G. K. , de Carlos Back I. , Moreno Y. M. F. 
Published: February 2020

AbstractBackgroundOver the past three decades, the prevalence rate of overweight and obesity has increased in survivors with congenital heart disease, and little is known about the body composition and its association with clinical characteristics and lifestyle factors.ObjectivesTo evaluate excess total‐body adiposity and central adiposity and, to describe associated factors.MethodsCross‐sectional study with children and adolescents who underwent procedure to treat congenital heart disease, from January to July 2017. Sociodemographic and clinical characteristics, and lifestyle factors (dietary intake, physical activity, and sedentary behavior) were assessed. Adiposity was assessed using air‐displacement plethysmography and waist circumference. Factors associated with excess total‐body adiposity and central adiposity were analyzed using logistic regression models.ResultsOf 232 patients, 22.4% were identified with excess total‐body adiposity and 24.6% with central adiposity. Significant factors positively associated with excess total‐body adiposity were intake of added sugar and trans fatty acids, adjusted for confounding factors. Similarly, lifestyle factors were positively associated with central adiposity: intake of added sugar and trans fatty acids, sedentary behavior, and family history of obesity.ConclusionsLifestyle factors were associated with excess total‐body adiposity and central adiposity. Assessment of body composition and healthy‐lifestyle counseling into outpatient care may be the key point to prevent obesity in children and adolescents with congenital heart disease.
Niche partitioning in congeneric scorpions
Authors: Goodman Aaron , Esposito Lauren 
Published: February 2020

AbstractSpecies in the scorpion genus Centruroides (Scorpiones: Buthidae) are good candidates for the study of ecological niche partitioning because of their habitat plasticity, widespread geographic distribution, and presence of cryptic species. Currently, three species belonging to three subgroups of Centruroides are distributed along the Isthmus of Tehuantepec in southern Mexico, presenting a rare opportunity to study niche partitioning within a single genus. We examined the environmental, substrate, and habitat conditions of Centruroides flavopictus, Centruroides gracilis, and Centruroides rileyi within Estación de Biología Los Tuxtlas, west of the Isthmus of Tehuantepec. Our results demonstrate habitat partitioning occurs along humidity, temperature, and elevational gradients: individuals of C. gracilis were found in habitats of low humidity and high temperature, and were predominantly found on the substrate in human‐mediated habitats; individuals of C. flavopictus were found in a larger range of humidity and temperature conditions within secondary and primary forest; and individuals of C. rileyi had the greatest habitat specificity, and were found in conditions of low temperature and high humidity on trees within primary forest. Furthermore, individuals of C. rileyi were found significantly higher in trees than those of C. flavopictus. This study represents the first example of niche partitioning within a genus of scorpion, and the first description of the ecological niche in an arboreal‐specialist scorpion.
Recommendations for home blood pressure monitoring in Latin American countries: A Latin American Society of Hypertension position paper
Authors: Villar Raúl , Sánchez Ramiro A. , Boggia José , Peñaherrera Ernesto , Lopez Jesús , Barroso Weimar Sebba , Barbosa Eduardo , Cobos Leonardo , Hernández Hernández Rafael , Octavio José Andrés , Parra Carrillo José Z. , Ramírez Agustín J. , Parati Gianfranco 
Published: February 2020

AbstractOut‐of‐office blood pressure (BP) monitoring appears to be a very useful approach to hypertension management insofar it allows to obtain multiple measurements in the usual environment of each individual, allows the detection of hypertension phenotypes, such as white‐coat and masked hypertension, and appears to have superior prognostic value than the conventional office BP measurements. Out‐of‐office BP can be obtained through either home or ambulatory monitoring, which provide complementary and not identical information. Home BP monitoring yields BP values self‐measured in subjects' usual living environment; it is an essential method for the evaluation of almost all untreated and treated subjects with suspected or diagnosed hypertension, best if combined with telemonitoring facilities, also allowing long‐term monitoring. There is also increasing evidence that home BP monitoring improves long‐term hypertension control rates by improving patients' adherence to prescribed treatment. In Latin American Countries, it is widely available, being relatively inexpensive, and well accepted by patients. Current US, Canadian, Japanese, and European guidelines recommend out‐of‐office BP monitoring to confirm and refine the diagnosis of hypertension.
Ambulatory blood pressure monitoring over 24 h: A Latin American Society of Hypertension position paper—accessibility, clinical use and cost effectiveness of ABPM in Latin America in year 2020
Authors: Sánchez Ramiro A. , Boggia José , Peñaherrera Ernesto , Barroso Weimar Sebba , Barbosa Eduardo , Villar Raúl , Cobos Leonardo , Hernández Hernández Rafael , Lopez Jesús , Octavio José Andrés , Parra Carrillo José Z. , Ramírez Agustín J. , Parati Gianfranco 
Published: February 2020

AbstractAccurate office blood pressure measurement remains crucial in the diagnosis and management of hypertension worldwide, including Latin America (LA). Office blood pressure (OBP) measurement is still the leading technique in LA for screening and diagnosis of hypertension, monitoring of treatment, and long‐term follow‐up. Despite this, due to the increasing awareness of the limitations affecting OBP and to the accumulating evidence on the importance of ambulatory BP monitoring (ABPM), as a complement of OBP in the clinical approach to the hypertensive patient, a progressively greater attention has been paid worldwide to the information on daytime and nighttime BP patterns offered by 24‐h ABPM in the diagnostic, prognostic, and therapeutic management of hypertension. In LA countries, most of the Scientific Societies of Hypertension and/or Cardiology have issued guidelines for hypertension care, and most of them include a special section on ABPM. Also, full guidelines on ABPM are available. However, despite the available evidence on the advantages of ABPM for the diagnosis and management of hypertension in LA, availability of ABPM is often restricted to cities with large population, and access to this technology by lower‐income patients is sometimes limited by its excessive cost. The authors hope that this document might stimulate health authorities in each LA Country, as well as in other countries in the world, to regulate ABPM access and to widen the range of patients able to access the benefits of this technique.
Efficacy and safety of renal denervation for the management of arterial hypertension: A systematic review and meta‐analysis of randomized, sham‐controlled, catheter‐based trials
Authors: Stavropoulos Konstantinos , Patoulias Dimitrios , Imprialos Konstantinos , Doumas Michael , Katsimardou Alexandra , Dimitriadis Kyriakos , Tsioufis Costas , Papademetriou Vasilios 
Published: February 2020

AbstractDespite the availability of a numerous antihypertensive agents, hypertension treatment and control rates remain low in many countries. The role of the sympathetic nervous system has long been recognized, but recent sham control renal denervation studies demonstrated conflicting results. In this reviewe paper, the authors performed a systematic review and meta‐analysis to examine outcomes of sham‐controlled studies utilizing new technologies and procedures. Six published randomized, sham‐controlled studies were included in this meta‐analysis. Of those, three trials used the first‐generation radiofrequency renal denervation device and technique and the other three used second‐generation devices and techniques. In total, 981 patients with hypertension were randomized in all 6 trials to undergo renal denervation (n = 585) or sham procedure (n = 396). Overall, renal denervation resulted in a decrease of 24‐hours systolic ambulatory blood pressure (ABP) by 3.62 mm Hg (95% CI: −5.28‐−1.96; I2 = 0%), compared to sham procedure (GRADE: low). Renal denervation also reduced daytime systolic ABP by 5.51 mm Hg (95% CI: −7.79‐−3.23; I2 = 0%), compared to sham procedure but not nighttime systolic ABP. Office systolic blood pressure was reduced by 5.47 mm Hg (95% CI −8.10‐−2.84; I2 = 0%), compared to sham control. Further analysis demonstrated that second‐generation devices were effective in reducing blood pressure, whereas the first‐generation devices were not. These results indicate that effective renal denervation can result in significant and clinically meaningful blood pressure reduction. The second‐generation devices provide better renal nerve ablation.
STAT1 and its related molecules as potential biomarkers in Mycobacterium tuberculosis infection
Authors: Yi Xing‐hao , Zhang Bo , Fu Yu‐rong , Yi Zheng‐jun 
Published: February 2020

AbstractTuberculosis (TB) is a severe infectious disease that seriously endangers human health. The immune defence mechanism of the body against TB is still unclear. The purpose of this study was to find the key molecules involved in the immune defence response during TB infection, and provide reference for the treatment of TB and further understanding of the immune defence mechanism of the body. Data from GSE83456 were downloaded from GEO data sets for analysis, and a total of 192 differentially expressed genes were screened out. Most of these genes are enriched in the interferon signalling pathway and are defence response–related. We also found that STAT1 plays an important role in the immune defence of TB infection and it is one of the key genes related to interferon signalling pathway. STAT1‐related molecules including hsa‐miR‐448, hsa‐miR‐223‐3p, SAMD8_hsa_circRNA 994 and TWF1_hsa_circRNA 9897 were therefore screened out. Furthermore, expression levels of hsa‐miR‐448 and hsa‐miR‐223‐3p were then verified by qRT‐PCR. Results showed that both hsa‐miR‐448 and hsa‐miR‐223‐3p were down‐regulated in plasma from patients with pulmonary TB. Taken together, our data indicate that an mRNA‐miRNA‐circRNA interaction chain may play an important role in the infection of MTB, and STAT1 and related molecules including hsa‐miR‐223‐3p, has‐miR‐448, SAMD8_hsa_circRNA994 and TWF1_hsa_circRNA9897 were identified as potential biomarkers in the development of active TB.
Long non‐coding RNAs: How to regulate the metastasis of non–small‐cell lung cancer
Authors: Fang Cheng , Wang Lixin , Gong Chenyuan , Wu Wenbin , Yao Chao , Zhu Shiguo 
Published: February 2020

AbstractNon–small‐cell lung cancer (NSCLC) has become the most lethal human cancer because of the high rate of metastasis. Hence, clarifying the molecular mechanism underlying NSCLC metastasis is very important to improve the prognosis of patients with NSCLC. Long non‐coding RNAs (LncRNAs) are a class of RNA molecules longer than 200 nucleotides, which can participate in diverse biological processes. About 18% of human LncRNAs were recently found to be associated with tumours. Many studies indicated that aberrant expression of LncRNAs played key roles in the progression and metastasis of NSCLC. According to the function in tumours, LncRNAs can be divided into two classes: oncogenic LncRNAs and tumour‐suppressor LncRNAs. In this review, we summarized the main molecular mechanism of LncRNAs regulating NSCLC metastasis, including three aspects: (a) LncRNAs interact with miRNAs as ceRNAs; (b) LncRNAs bind with target proteins; and (c) LncRNAs participate in the transduction of different signal pathways. Then, LncRNAs can exert their function to regulate the metastasis of NSCLC through influencing the progression of epithelial‐mesenchymal transition (EMT) and the properties of cancer stem cell (CSC). But, it is necessary to do some further research to demonstrate the LncRNAs particular regulatory mechanism of inhibiting the metastasis of NSCLC and explore new drugs targeting LncRNAs.
Evolution of a conspicuous melanin‐based ornament in gulls Laridae
Authors: Minias Piotr , Janiszewski Tomasz 
Published: February 2020

AbstractMelanin‐ and carotenoid‐based ornaments often signal different aspects of individual quality or similar components of quality under different environmental conditions and, thus, they may become evolutionarily integrated into a composite sexual trait. On the other hand, functionally and developmentally different characters (e.g. coloration characters of different developmental origin) are more likely to evolve independently from each other than more similar traits. Here, we examined evolutionary correlations between the occurrence of a conspicuous melanin‐based ornament (hood) and carotenoid‐based bare‐part ornaments within gull family. We also aimed to identify major ecological, life‐history and biogeographical predictors of hood occurrence and reconstruct evolutionary history of this ornament. We found that hood occurrence was associated with red or dark coloration of unfeathered traits (bill and legs), whereas combinations of hood with yellow carotenoid‐based coloration of integument were evolutionarily avoided. Also, hood occurrence correlated negatively with the occurrence of other melanin‐based plumage character (mantle). Breeding latitude and habitat were identified as major predictors of hood occurrence in gulls, as hoods were recorded more frequently in low‐latitude and inland (rather than marine) species. Finally, our analysis provided support for evolutionary lability in hood occurrence, with a dominance of transitions towards hood loss in the evolutionary history of gulls. The results of our study provide one of the first evidence for a correlated evolution of melanin‐ and carotenoid‐based ornaments in an avian lineage, which supports evolutionary modularity of developmentally and functionally different coloration traits.
Phenotypic and genetic differentiation between diadromous and landlocked puyen Galaxias maculatus
Authors: Rojo Javier Hernán , Fernández Daniel Alfredo , Figueroa Daniel Enrique , Boy Claudia Clementina 
Published: February 2020

AbstractThis study reports the phenotypic and genetic differences between individuals of puyen Galaxias maculatus from two sites in the same river basin in Tierra del Fuego National Park, southern South America. Individuals from the two sampling sites presented morphometric and genetic differences. The morphometric differences indicated that individuals from Laguna Negra (LN) were short and more robust and had large eyes, whereas those from Arroyo Negro (AN) were thin and elongated and had small eyes. Genetic differences showed that AN individuals had a greater genetic structuration and an older demographic history than LN individuals. The results of this study affirmed that the individuals from the two sampling sites belong to different populations with a high degree of isolation. The demographic history could indicate that the individuals of G. maculatus which migrated to northern areas during the last glaciation settled in the Beagle Channel after its formation. The LN population could have originated after the retreat of the glaciers, migrating from AN.
Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ
Authors: Yang Jie , Gong Yafan , Cai Jingzeng , Zheng Yingying , Liu Honggui , Zhang Ziwei 
Published: February 2020

AbstractChlorpyrifos is a poisonous pesticide that is highly toxic to fish and aquatic organisms. However, there are fewer reports about how chlorpyrifos influences the redox balance of immune cells. Herein, the head kidney tissue treated with chlorpyrifos to do transcriptome analysis and TCR γ was screened out. Subsequently, we established TCR γ knockdown and overexpression carp head kidney lymphocyte models, respectively, by using RNA interference and pcDNA3.1. Real‐time PCR, fluorescent staining, oxidation and antioxidant kit were used to detect the related factors. We found that TCR γ knockdown significantly increased the mRNA expression of HSP70 and HSP90 and decreased the mRNA expression of SOD and CAT. Meanwhile, TCR γ knockdown reduced the activities of GSH, GSG‐PX, T‐AOC, CAT and SOD and increased the content of MDA and H2O2 and activities of iNOS. Adverse results were obtained in TCR γ overexpression group. Additionally, TCR γ knockdown significantly increased the mRNA expression of IFN‐γ, IL‐1β, IL‐8, IL‐10, Nrf2 and NF‐κB, but relieved TCR γ overexpression, in which the process of inflammation was activated. Our results reported here indicated that chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ, and HSPs play potential protective role in entire process.