bluuebunny/crossref_metadata_2025_split huggingface dataset:
More potential risk factors for implant in patients with type 2 diabetes were detected by proteomics in addition to hyperglycemia.
Authors: Tang Dezheng , Wang Entang , Xu Yifan , Liang Chao , Liu Changying , Lin Xiao , Li Jun 
Published: March 2020

Abstract
Background: It is commonly accepted glycemic control can decrease the negative effects of implant in type 2 diabetes mellitus (T2DM) patients. Whether the remaining pathological changes besides hyperglycemia caused by T2DM will affect implant-bone integration during the healing period has remained unclear. This study aims to determine whether other risk factors besides hyperglycemia lead to failed osseointegration in T2DM patients during healing period. Methods: First, we compared the success rate between T2DM patients and non-T2DM patients during the healing period at our center. Bone marrow mesenchymal stem cells (BMSCs) were cultured from all enrolled subjects. Then, proteomics was used to detect differentially expressed proteins (DEPs) among the DM failure (DM-F), DM success (DM-S) and control (Con) groups. Additionally, the relationship between expression of the nine target DEPs and glucose concentration in media was investigated. Results: Significantly higher failure rates in T2DM patients were found. Fifty-two DEPs were found in DM-F group compared with DM-S group. Seventy-three DEPs were found in DM-F group compared with the Con group. Forty-three DEPs were found in DM-S group compared with Con group. Four target DEPs was influenced by glucose, while the other five expressed the same in different glucose concentration media. DEPs in DM-F group may affect the biological function and regulatory potential of BMSCs through gene ontology annotation and functional enrichment analysis. Conclusions: DEPs in DM-F group can be potential risk factors and intervention targets for dental implant in T2DM patients. More potential risk factors affect implant-bone integration besides hyperglycemia.
The effects of the primary health care providers’prescription behavior interventions to improve the rational use of antibiotics: a systematic review
Authors: Yao Lu , Yin Jia , Sun Qiang , Huo Ruiting , Yang Ding , Shen Liyan , Wen Shuqin 
Published: March 2020

Abstract
Background: Irrational antibiotic use, especially at primary health care institutions accelerates the spread of antibiotic resistance (ABR). It is important to systematically review the effects of interventions at the primary health care to give evidence for future studies about interventions of providers’ antibiotic prescriptions. A systematic review was conducted to explore the effects of interventions targeted to the primary health care providers for improving the rational use of antibiotics. Methods: The literatures were searched in Ovid Medline, Web of Science, PubMed, Cochrane Library, and two Chinese databases with a time limit from January 1st, 1998 to December 1st, 2018. Only articles in the English and Chinese language were considered. Studies had to be the design of randomised control trial, controlled before-and-after studies or interrupted time series. Outcomes had to measure the change in providers’ behaviors. The Cochrane Collaboration criteria was used to assessed the risk of bias of the studies by two reviews. Narrative analysis was performed. Results: Of 4422 studies identified, we included 17studies. Most studies were conducted in the Europe or the United States and 4 of the studies were conducted in low-income and-middle-income countries (LMICs). Most studies had moderate to high risk of bias. There was moderate-strength evidence that interventions targeted at primary health care providers’ prescription behaviors were associated with decreases in antibiotic prescribing and promote the rational use of antibiotic.Conclusions: Provider-targeted interventions in primary health care could decrease the antibiotic prescription and promote the providers’ behaviours of irrational use of antibiotic However, we cannot compare the effects between different interventions because of heterogeneity of interventions and outcomes.
MiR-186 serves as a tumor suppressor in lung adenocarcinoma cells by down-regulation of Shp2 gene and inhibiting PI3K/Akt/mTOR signaling pathway
Authors: Yan Shao-Hui , Xu Shu-Feng , Zheng Lei , Kang Li-Ying , Cao Jun-Li , Wang Ping , Gao Liming 
Published: March 2020

Abstract
Background The study aimed to investigate the effect and mechanism of miR-186, which targets protein tyrosine phosphatase (Shp2) PI3K/Akt/mTOR signaling pathway, on the biological characteristics of lung adenocarcinoma cells. Methods In this experimental study, Human lung adenocarcinoma cell line SPC-A-1 was grouped as Blank group, negative control (NC) group, miR-186 mimic group, miR-186 inhibitor group, si-Shp2 group and miR-186 inhibitor+si-Shp2 group. Results The results showed that miR-186 can target and down-regulate the expression of Shp2 gene. Compared with the Blank group, levels of Shp2, N-cadherin and Bcl-2 and level of PI3K/p-PI3K, Akt/P-Akt, mTOR/p-mTOR as well as cell proliferation, migration and invasion ability and the proportion of cells in S phase significantly decreased in the miR-186 mimic group and the si-Shp2 group, while the levels of E-cadherin and Bax as well as the proportion of cells in G1 phase and cell gene and mediates apoptosis rate increased significantly (all P < 0.05). Compared with the miR-186 inhibitor group, the miR-186 inhibitor + si-Shp2 group showed similar trend in all parameters with the comparison above (all P < 0.05). Conclusions The overexpression of miR-186 can down-regulate Shp2 gene expression, further inhibit the proliferation, invasion and migration and promote apoptosis of lung adenocarcinoma cells by inhibiting the activation of PI3K/Akt/mTOR signaling pathway.
Digital Accuracy in Comuputer Guided Surgery a Multicentre Prospective Study
Authors: Luongo Fabrizia , Lerner Henriette , Gesso Camilla , Motroni Alessandro , Sormani Alice , Kalemaj Zamira , Polizzi Giovanni , Luongo Giuseppe 
Published: March 2020

Abstract
Background Digital technologies are becoming an irreplaceable assistance for a correct diagnosis and treatment planning of the dental therapy. Modern powerful software are able to match information coming from different sources (CBCT, IOS, lab and face scanners) creating a virtual patient where all the treatment options can be accurately tested. Guided surgery is based on the possibility to use these instruments to transfer the ideal plan directly into the operating theatre. However even in the hands of skilled operators there is always a difference between the position of the implant as determined on the virtual planning and the real position after the surgery. The aim of this multicenter prospective study is to test the advantages of the standardization of the digital workflow on the accuracy of the procedure. Methods 21 patients, for a total of 61 implants, have been treated in three different centers by operators with particular expertise in guided surgery. The accuracy of the procedure has been evaluated matching two files: the first from the pre-treatment virtual planning and the second from the optical impression taken immediately after the surgery. The digital workflow has been standardized using the same software for the planning, the same high quality manufacturing process, intraoral scanner and software for the overlapping procedure. Results All the implants showed an excellent primary stability. No intraoperative complications have been reported. The overlapping of the two files resulted in a mean angular deviation of 2.94°, a mean linear deviation at the platform of 0,73 mm and a mean vertical deviation at the apex of 0.01mm. Conclusions Almost the totality of the cases showed a better accuracy compared with the previous reported data of the literature. These results might be related with the standardization of the process so that following a precise protocol is mandatory for a safe use of this technique.
Communicating Prevention in the COVID-19 Epidemic: Case Study on Macau SAR
Authors: Au Anna Weng Ian 
Published: March 2020

Abstract
Background As the COVID-19 epidemic is unfolding, an infodemic has also developed. With commonplace social media usage, a massive amount of information and misinformation is spreading at the same time. Irrational responses are reported worldwide – face mask and detergent shortages, panic shopping, and xenophobia, which could possibly be a result of ineffective science communication. Using Macau SAR for a case study, this article sets out to understand effective prevention communication. Methods An observation study was conducted to understand Macau’s prevention communication in the COVID-19 epidemic. Results Macau has imposed a wide range of preventions, covering most dimensions of daily life. Lessons are learned in risk perception, language used, science communication, digital literacy, information flow, and support in STEM advancement. Conclusions Macau’s prevention communication is not perfect, but Macau’s experience offers insights to European authorities on how to contain the epidemic and minimize undesirable outcomes as the epidemic is just beginning in Europe.
Infection after knee arthroplasty in an immunosuppressive patient caused by Mycobacterium senegalense: a case report and literature review
Authors: Tian Lei , Wang Feng , Chen Zhongju , Zeng Yifan , Zhu Xuhui , Gong Lu , Wang Xueman , Sun Ziyong 
Published: March 2020

Abstract
Background The infection caused by nontuberculous Mycobacterium (NTM) is gradually increasing in both hospital-acquired infection and community-acquired infection. The most common NTM mainly includes Mycobacterium fortuitum group, the M. chelonae/abscessus group and the M. smegmatis group. The infection caused by the third biological variety in M. fortuitum group is rarely reported worldwide.Case presentation A 33-year-old female patient with 8-year history of rheumatoid arthritis underwent a right knee arthroplasty and developed postoperative infection. In hospital A, debridement was carried out to preserve the prosthesis joint. M. fortunatum was cultured in biopsy tissue taken out during operation and puncture fluid. Unfortunately, it is impossible to carry out the drug sensitivity test of Mycobacterium in hospital A. Local doctors used amikacin and levofloxacin to treat the patient empirically. In order to treat the infection accurately, the patient went to hospital B (a specialized hospital of tuberculosis) for drug sensitivity test. According to the results of drug sensitivity, cefoxitin, clarithromycin, amikacin and moxifloxacin were used in combination treatment. After two weeks of treatment, there was no significant remission, so the patient went to hospital C (a large teaching hospital) for joint prosthesis removal and bone cement frame transfer operation, and anti-infection treatment was carried out according to the drug sensitivity test results. One month later, the patient's condition improved significantly and the wound healed.Conclusions NTM should be considered as main pathogens in immunosuppressive patients when the wound did not heal due to infection. Simple antibiotic treatment is not good for deep abscess, but treatment combined with surgical debridement and appropriate antibiotics is obviously effective.
Rapid ultrasensitive diagnosis of pneumonia caused by Acinetobacter baumannii using a combination of enrichment and phage-based qPCR assay
Authors: Luo Jun , Jiang Mengwei , Xiong Jin , Li Junhua , Wei Hongping , Yu Junping 
Published: March 2020

Abstract
Background Accurate and rapid identification of ventilator associated or nosocomial pneumonia caused by Acinetobacter baumannii ( A. baumannii ) could improve the treatment.Methods In current study, we developed a phage-based real-time quantitative PCR (qPCR) combined with enrichment culture for rapid and specific detection of viable A. baumannii in sputum from lung infections. Through short-term plate incubation, bacteria can be enriched and the DNA polymerase reaction disturbance of sputum can be decreased greatly. This approach is based on detecting phage replication in live A. baumannii cells through Taqman qPCR.Results Through the built detection system, down to 1 CFU of A. baumannii can be detected within 6 h in spiked sputum samples without any steps of bacteria isolation and DNA extraction. The established method was then applied to detecting both A. baumannii in simulated sputum with 100% agreement with the spiked amount of the bacteria and one clinical sputum sample from an 80-year-old male lung infection patient caused by A. baumannii with perfect accuracy, demonstrating that the assay developed in this study has the merits of high rapidity, high sensitivity, good specificity and being able to detect live bacteria not dead bacteria.Conclusions The assay is a potentially clinical method for diagnosis of bacterial pneumonia infection caused by A. baumannii or other bacterial infection in sputum or complicated samples through switching to other types of phages.
Determining the usefulness of serum hyaluronic acid levels as a predictor of progression of hand osteoarthritis: longitudinal analysis from the Iwaki cohort
Authors: Saruga Tatsuro , Sasaki Eiji , Inoue Ryo , Chiba Daisuke , Ota Saiya , Iwasaki Hiroki , Uesato Ryoko , Nakaji Shigeyuki , Ishibashi Yasuyuki 
Published: March 2020

Abstract
Background: Hand osteoarthritis (HOA) causes a significant disfunction in patient’s daily life. The predicting factor of hand osteoarthritis has been unknown. We aimed to investigate the usefulness of serum hyaluronic acid (sHA) levels in predicting progression of HOA from a 6-year longitudinal epidemiological study.Design: In 2008, a total of 417 participants in the Iwaki cohort were followed over 6 years. Hand radiographs were taken at baseline and follow-up and scored according to Kellgren–Lawrence grades and Kallman score for 15 joints. Based on the presence of osteoarthritis, participants were classified into HOA and non-HOA groups. Levels of serum hyaluronic acid (sHA) at baseline were determined by ELISA. Spearman’s correlation coefficients between levels of sHA, total number of involved joints, and Kallman score were estimated. Factors related to increasing number of involved joints over a period of six years were analyzed by liner regression analysis.Results: The prevalence of hand osteoarthritis was 19.9% at baseline and the number of joints involved was 3.6 ± 2.1. Levels of sHA in the HOA group at baseline were significantly higher than non-HOA group (p<0.0001), and correlated with the number of involved joints (r=0.399, p<0.0001) and Kallman score (r=0.540, p<0.0001). Progression rate was 55.4% and development rate was 19.1% over six years. In HOA group, the number of involved joints increased by 4.9 ± 2.3. Associated factors were age (p<0.0001) and higher levels of sHA (p<0.0001) at baseline.Conclusions: Higher levels of sHA correlated with number of involved joints and Kallman score at baseline. In the longitudinal study, Levels of sHA predicted progression of HOA over six years.
The Impact of Real Exchange Rate Undervaluation on Economic Growth: Evidence from Panel Cointegration Approach
Authors: Seraj Mehdi , Coskuner Cagay , Akadiri Seyi Saint , Bahadori Negar 
Published: March 2020

Abstract
This study revisited Dani Rodrik (2008) work on real exchange rate undervaluation and economic growth by using the Fully Modified Ordinary Least Square (FMOLS) and Dynamic Ordinary Least Square (DOLS). This research, to the best of authors' knowledge, is the first to use FMOLS and DOLS approach to empirically evaluate Rodrik work on the real exchange rate and economic growth using a Panel periodic data (six sets of five years) of 82 countries throughout 1990 to 2018. We used the Balassa Samuelson method to estimate the predicted real exchange rate and real exchange rate undervaluation. Finally, the study is in support of Rodrik conclusion that, real exchange undervaluation has a significant impact on the economic growth of the developing economies and statistically insignificant in the developed economies.
Construction and Clinical Verification of Cytoplasm Protein GFAP as Circulating Tumor Cell Separation target for Pediatric Neuroepithelial Tumors
Authors: Zhao Yang , Jiang Feng , Wang Qinhua , Wang Baocheng , Han Yipeng , Yang Jian , Dong Xiaoshu , Wang Jiajia , Wang Kai , Ao Junping , Guo Xunxiang , Liang Xiaofei , Ma Jie 
Published: March 2020

Abstract
BACKGROUND: Pediatric Neuroepithelial Tumors (NT) are one of the most prevalent diseases among children. Developing a highly efficient cerebrospinal fluid (CSF) detection system with diagnosis and prediction function is very important. Circulating tumor cell (CTC) in CSF is a good choice. In contrast to the past use of epithelial EpCAM as CTC separation target, an cytoplasm protein of GFAP antibody was first selected to construct highly-sensitive immunomagnetic liposomes (IMLs). The validation and efficiency of this system in capturing CTCs for NT were measured both in vitro and in vivo. The associations between the numbers of CTCs in patients with their clinical characteristics were further analyzed. RESULTS: Our data show that CTCs can be successfully isolated from CSF and blood samples from 29 children with NT. The numbers of CTCs in CSF were significantly higher than those in blood. The level of CTCs in CSF was related to the type and location of the tumor rather than its stage. Genetic testing in GFAP CTC-DNA by sanger sequencing, q-PCR and NGS methods indicated that the isolated CTCs (GFAP+/EGFR+) are the related tumor cell. For example, the high expression of NPR3 gene in CSF CTC was consistant with tumor tissue. CONCLUSIONS: GFAP-IML isolation of CTCs, combined with an EGFR immunofluorescence assay of antitumor marker, can serve as a brand-new method for the identification of CTCs for brain tumors. Via lumbar puncture, a minimally invasive procedure, this technique can be clinically significant in diagnosis and efficacy assessments of pediatric NT.
Exploration of human movement processing in autism spectrum disorder
Authors: Nijhof Dewy , Regener Paula , Kim Naree , Tohka Jussi , Kauppi Jukka-Pekka , Jang Seonhee , Pollick Frank 
Published: March 2020

Abstract
Evidence regarding whether people diagnosed with Autism Spectrum Disorder (ASD) show differences in processing human movement is mixed, with recent findings being both in support and in contrast. To provide more clarity, this study utilised inter-subject correlation (ISC) analysis to quantify similarities in human movement processing in fMRI responses to a naturalistic movie paradigm portraying ballet dance between adults with and without ASD. Moreover, similarities within each individuals fMRI responses over repeated viewings were quantified to see if responses were stable and if idiosyncratic patterns could be determined. One group difference found was a cluster in the right posterior cingulate showing significantly lower ISC for the ASD group, corresponding to extensive literature showing atypical activity in the Default Mode Network in ASD. Idiosyncratic brain activation patterns were found only for a subset of ASD individuals, opening up the possibility to subtypes and a possible link to severity in symptoms.
Study on mechanism of Tim-3 on immune escape in benzene-induced acute myeloid leukemia
Authors: Ning qiong , li xiangxin , Jian Xiangdong , He Xiaopeng 
Published: March 2020

Abstract
To study the mechanism of Tim-3 on immune escape in benzene-induced acute myeloid leukemia (AML), to provide potential targets of clinical monitoring and intervention of hematological toxicity in benzene-induced AML . C3H/He mice were randomly divided into control group and experimental group. Serum levels of IL-12 in the experimental group were significantly lower than that in the control group. Serum levels of TGF-β1 in the experimental group were significantly higher than that in the control group( p <0.05). The proportion of Tim-3 positive CD14 + monocytes of bone marrow and spleen in the experimental group were both significantly higher than that in the control group ( p <0.05) by Flow cytometry (FCM). Compared with the control group, the expression of Tim-3 on (M1+M2) macrophages of bone marrow in the experimental group significantly increased by immunofluorescence assay. The expression of type M2 macrophages in (M1+M2) macrophages of bone marrow and spleen tissues in the experimental group were both higher than that in the control group. The expression levels of p-PI3K, p-AKT and p-mTOR in the experimental group were all significantly higher than that in the control group. Tim-3 was highly expressed in macrophages in benzene-induced AML. It promoted the activation of PI3K/AKT/mTOR signaling pathway, stimulated the secretion of anti-inflammatory cytokines, and inhibited the secretion of pro-inflammatory cytokines. High expression of Tim-3 changed the phenotype and function of macrophages by promoting the macrophages polarization, thus inducing negative immune response in the tumor microenvironment and tumor immune escape.
Identification of hub genes and a prognostic model associated with ovarian cancer
Authors: Liu Jinhui , Jiang PinPing , Meng Huangyang , Li Siyue , Nie Sipei , Rui Sun , Jing Yang , Cheng Wenjun 
Published: March 2020

Abstract
Background: Ovarian cancer (OC) is one of the most lethal gynecological cancers worldwide. The pathogenesis of the disease and outcomes prediction of ovarian cancer patients remain largely unclear. This study aimed to explore the key genes and biological pathways in ovarian carcinoma development, as well as construct a prognostic model to predict patients’ overall survival. Results: We identified 164 up-regulated and 80 down-regulated differentially-expressed genes (DEGs) associated with ovarian cancer. GO term enrichment showed DEGs mainly correlated with spindle microtubes. For KEGG pathways, cell cycle was mostly enriched for the DEGs. The PPI network yielded 238 nodes and 1284 edges. Top three modules and 10 hub genes were further filtered and analyzed. Three candidiate drugs targeting for therapy were also selected. Thirteen OS-related genes were selected and an eight-mRNA model was present to stratify patients into high- and low-risk groups with significantly different survival. Conclusions: The identified DEGs and biological pathways may provide new perspective on the pathogenesis and treatments of OC. The identified 8-mRNA signature has significant clinical implication for outcome prediction and tailored therapy guidance for OC patients.