bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome and metabolite analyses provide insights into zigzag-shaped stem formation in tea plants (Camellia sinensis)
Authors: Cao Hongli , Wang Feiquan , Lin Hongzheng , Ye Yijun , Zheng Yucheng , Li Jiamin , Hao Zhilong , Ye Naixing , Yue Chuan 
Published: February 2020

Abstract
Background: Shoot orientation is important for plant architecture formation, and zigzag-shaped shoots are a special trait found in many plants. Zigzag-shaped shoots have been selected and thoroughly studied in Arabidopsis ; however, the regulatory mechanism underlying zigzag-shaped shoot development in other plants, especially woody plants, is largely unknown. Results: In this study, tea plants with zigzag-shaped shoots, namely, Qiqu (QQ) and Lianyuanqiqu (LYQQ), were investigated and compared with the erect-shoot tea plant Meizhan (MZ) in an attempt to reveal the regulation of zigzag-shaped shoot formation. Tissue section observation showed that the cell arrangement and shape of zigzag-shaped stems were aberrant compared with those of normal shoots. Moreover, a total of 2175 differentially expressed genes (DEGs) were identified from the zigzag-shaped shoots of the tea plants QQ and LYQQ compared to the shoots of MZ using transcriptome sequencing, and the DEGs involved in the “Plant-pathogen interaction”, “Phenylpropanoid biosynthesis”, “Flavonoid biosynthesis” and “Linoleic acid metabolism” pathways were significantly enriched. Additionally, the DEGs associated with cell expansion, vesicular trafficking, phytohormones, and transcription factors were identified and analysed. Metabolomic analysis showed that 13 metabolites overlapped and were significantly changed in the shoots of QQ and LYQQ compared to MZ. Conclusions: Our results suggest that zigzag-shaped shoot formation might be associated with the gravitropism response and polar auxin transport in tea plants. This study provides a valuable foundation for further understanding the regulation of plant architecture formation and for the cultivation and application of horticultural plants in the future.
Contralateral C7 Transfer to Axillary and Median Nerves in Rats with Total Brachial Plexus Avulsion
Authors: Liu Yuzhou , Xiao Feng , Zhuang Yongqing , Lao Jie 
Published: February 2020

Abstract
Background: Contralateral cervical 7 nerve (cC7) was used to repair two recipient nerves simultaneously for patients with total brachial plexus avulsion (TBPA). Objective: To evaluate the effect of cC7 transfer to axillary and median nerves in rats with TBPA. Methods: Eighty S-D rats were divided into 4 groups randomly on average. Group A: cC7 － median nerve, Group B: cC7 － axillary nerve, Group C: cC7 － median and axillary nerves, Group D: TBPA without repair. The evaluation tools included behavioral test, electromyogram (EMG), cross-sectional area of muscle fiber, nerve fiber count and gene expression. Results: The effective rates of EMG were 90% and 70% in Flexor Carpi Radialis (FCR) in Group A and C, while 70% and 60% in deltoid (DEL) in Group B and C. In behavioral test, the difference of effective rates between groups was not significant. The mean cross-sectional area of FCR in Group A or C was significantly larger than that in Group D. Either the number of median or axillary nerve fibers in Group A, B or C was statistically more than that in Group D. No matter for FCR or DEL, there were no significant differences in the ratios of relative expression of Muscle Atrophy F-box （ MAFBOX ） and Muscle RING Finger 1 （ MURF1 ） among these groups. Conclusion: Compared with cC7 transfer to median nerve, cC7 transfer to both median and axillary nerves did not affect median nerve recovery. The deltoid muscle also could be restored. The recovery proportion of axillary nerve was less than that of median nerve.
Analysis of the Influencing Factors of the Medical Service Capacity in Key Clinical Specialties at the County Level Based on TOPSIS
Authors: Wang Kaixuan , Guo Jin-Ling , Wang Wei , Zhao Shui-Chang , Li Miao-Jun , Wang Zhi-Long , Sun Ning , Bi Chun-Xiao , Ma Xin-Sheng 
Published: February 2020

Abstract
To acknowledge the medical service capacity of the first batch of county-level key clinical specialties in Henan province, analyze the influencing factors, and make targeted suggestions. TOPSIS method was used to evaluate the medical service capacity comprehensively, and the influencing factors were analyzed by simple linear regression and multiple linear regression. In the multiple linear regression model, the basic conditions, including the total investment funds during the construction period, net usable area of beds and the ratio of bed to nurse, the quality of care—the compliance rate of major clinical diagnosis and pathological diagnosis, and the personnel training—the number of technical promotion trainees, of the first county-level clinical specialties, have a significant influence upon the medical service capacity; while, the actual number of beds, the ratio of doctors and nurses, the proportion of annual expert visits, the number of technical promotion training and sending of learners, percentage of drug expenditure and core journal articles have limited effect. On the basis of the ensured allocation of resources and capital investment, more attention should be paid to personnel training and appropriate technology promotion, and the medical care quality is supposed to be always insisted on, to promote the overall improvement of medical service capacity in county hospitals.
Simultaneous detection of multiple fly-borne bacterial pathogenic microorganisms by the reverse line blot hybridization assay
Authors: Ma Yonghua , Luo Jianxun , Gou Huitian , Sun Xiaolin , Li Mei , Teng Yuxiao 
Published: February 2020

Abstract
Background: As a widespread health pest, flies can carry more than 100 kinds of path ogenic microbes to threat human health, resulting in a wide range of disease infection and transmission. The aim of this study was to develop a sensitive, reliable and rapid method for the simultaneous detection of multiple fly-borne bacterial pathogenic microorganisms, in order to effectively prevent and control fly-borne bacterial diseases. Results: PCR-RLB method could directly and accurately detect fly-borne bacteria species corresponding of 7 species-specific probes. The sensitivity of PCR-RLB was significantly higher (about 100 times) than that of PCR. At the same time, the membrane binding oligonucleotide species-specific probes prepared in RLB detection technology can be reused for detection of bacteria after washing with 0.5 M EDTA, which greatly improves the detection efficiency. This method was used to detect the intestinal pathogens carried by flies in four different areas of Lanzhou city. In 106 groups of samples from different areas, The numbers of carrying seven bacterial strains were 2 (S. aureus), 52 (S. flexneri), 0 (A. caviae), 3% (V. vulnificus), 56 (S. enterica), 1 (P. vulgaris) and 33 (Y. enterocolitica), respectively. Their proportions of 7 bacterial strains carried by houseflies were 1.23% ( S. aureus ), 32.1% ( S. flexneri ), 0% ( A. caviae ), 1.85% ( V. vulnificus ), 34.57% ( S. enterica ), 0.62% ( P. vulgaris ) and 20.37% ( Y. enterocolitica ), respectively. The results showed that there was a high correlation between the bacteria carrying rate of flies and the health environment in urban areas. S. enterica , S. flexneri and Y. enterocolitica accounted for the overwhelming majority of the seven pathogenic strains carried by houseflies. This indicated that houseflies played an important role in the transmission of intestinal infectious diseases. S. aureus and V. vulnificus were carried by houseflies near the hospital area indicates that hospitals should do well in killing and controlling flies and further strengthen the prevention and control of fly-borne bacterial diseases. Conclusion: The developed PCR-RLB assay have potential clinical application in the simultaneous detection of fly-borne bacterial species.
Eosinophil-to-monocyte ratio is a potential biomarker in the prediction of functional outcome among patients with acute ischemic stroke
Authors: Yu Shuhong , Luo Yi , Zhang Tan , Huang Chenrong , Fu Yu , Zhang Qiang , Zeng Fangyue , Huang Hao , Zhang Chunyuan , Guo Zhiliang 
Published: February 2020

Abstract
Background and PurposeIt has been showed that eosinophils are decreased and monocytes are elevated in patients with ischemic stroke, but the impact of eosinophil-to-monocyte ratio (EMR) on clinical outcomes among patients with acute ischemic stroke remains unclear. The aim of this study is to determine the relationship between EMR on admission and three-month poor functional outcome in patients with acute ischemic stroke.MethodsA total of 521 consecutive patients admitted to our hospital within 24 hours after onset of acute ischemic stroke were prospectively enrolled and categorized in terms of quartiles of EMR on admission between August 2016 and September 2018. The endpoint was the poor outcome defined as modified Rankin Scale score of 3 to 6 at month 3 after admission.ResultsAs EMR decreased, the risk of poor outcome increased (P<0.001). Logistic regression analysis revealed that EMR was independently associated with poor outcome after adjusting potential confounders (odds ratio, 0.10; 95% CI, 0.03–0.36; P=0.0004), which is consistent with the result of EMR (quartile) as a categorical variable (odds ratio, 0.24; 95% CI, 0.10–0.53; Ptrend<0.0001). A non-linear relationship was detected between EMR and poor outcome, whose point was 0.28. Subgroup analyses further confirmed these associations. The area under the curve of EMR for the prediction of poor outcome in receiver operating characteristic analysis was 0.653 (95% CI, 0.603–0.703; P=0.003). Furthermore, the addition of EMR to conventional risk factors improved the predictive power for poor outcome (net reclassification improvement: 3.54%, P=0.230; integrated discrimination improvement: 2.11%, P=0.001).ConclusionEMR on admission was independently correlated with poor outcome in patients with acute ischemic stroke, suggesting that EMR may be a potential prognostic biomarker for ischemic stroke.
BVD protects against UVB-induced HaCaT keratinocytes photodamage through reactivating Nrf2 antioxidative stress signaling
Authors: Liangjuan Ma , Jie Gong , Yingdi Liu , Jing Ren , Fang Lin , Xiaohui Zhang , Yan You , Bai Bing xue , Yuzhen Li 
Published: February 2020

Abstract
Background : To investigate the protective role and mechanism of exogenous biliverdin (BVD) on ultraviolet B (UVB) irradiated human keratinocytes (HaCaT cells). Methods : Cultured HaCaT cells were divided into control group, UVB group, and BVD + UVB group. Morphological changes in the HaCaT cells were observed, the cell viability of each group was detected, the mean fluorescence intensity (reactive oxygen species, ROS) of the cells was detected, the superoxide dismutase (SOD) and malondialdehyde (MDA) levels of the cells were detected, and the protein expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), matrix metalloproteinase-1 (MMP-1), and matrix metalloproteinase-3 (MMP-3) were determined. Moreover, Nrf2 gene transfection was performed in HaCaT cells. Results : UVB irradiation induced apoptosis of HaCaT cells, decreased Nrf2 protein expression and increased MMP-1 and MMP-3 protein expression, which increased ROS and MDA levels and decreased SOD levels. Nrf2 gene enhancement increased UVB-irradiated HaCaT cell viability. Conclusion : Exogenous BVD plays a protective role in UVB-induced HaCaT cell damage, and this effect may be related to the Nrf2 antioxidant signaling pathway. Keywords : Biliverdin, UVB irradiation, Photo-damage，Nrf2，oxidativestress，HaCaT
A systematic review and meta-analysis of Job stress among Iranian nurses
Authors: Isfahani Parvaneh , Arefy Marzieh , Peyravi Saeed , Bahador Rasoul Corani , Afshari Mahnaz 
Published: February 2020

Abstract
Background: Job stress is the most important factor in reducing organizational productivity. Therefore, this study aimed to identify prevalence of job stress among nurses in Iran.Methods: This study was conducted using a meta-analysis. All published articles on prevalence of job stress among nurses in Iran until July 2018 were searched and collected using PubMed, Web of Science, Science Direct, CINAHL, PsychInfo, Scopus, Magiran, IranMedex and SID and Bibliography section of the retrieved papers and related journals. Finally, 22 empirical studies were reviewed using Comprehensive Meta-Analysis software.Results: The overall prevalence of job stress in Iranian nurses was 49.5% (95% CI 41.9%-59.1%). The highest job stress was recorded in Tehran in 2016, 96.4% (95% Cl: 90.3%-98.7%) and the lowest job stress was found in Kashan in 2011, 2.5% (95% Cl: 6.0%-1.0%). Significant statistical correlation was observed between job stress, year and sample size (p<0/05).Conclusion: job stress affects about half of nursing staff in Iran; thus, managers and policymakers should take serious measures to reduce prevalence of job stress among nurses.
The hazards of PM2.5 pollution: Empirical evidence from perinatal mortality in China
Authors: Li guangqin , Li Lingyu , Liu Dan 
Published: February 2020

Abstract
Background : Although the adverse effects of air pollution on health have aroused widespread concern in academia, there is little evidence about the impact of PM2.5 on perinatal mortality rates. Methods: Using the spatial analysis function of ArcGIS, we get the haze pollution data from the satellite remote sensing data. We adopt fixed effects model, spatial Durbin model (SDM) and the instrument variable method to investigate the causality between PM2.5 and perinatal mortality rates. Results: We find that PM2.5 has a significantly positive effect on perinatal mortality rates. A 1% increase of log-transformed average concentrations and maximum concentrations of PM2.5 result in 1.76‰ and 2.31‰ increase of perinatal mortality rates, respectively. In spatial econometrics analysis, we find PM2.5 has significant spatial autocorrelation characteristics. A 1% increase of concentrations of log-transformed average and maximum PM2.5 lead to a 2.49‰ and 2.19‰ increase of perinatal mortality rates, respectively. Using instrument variable method to deal with the endogeneity, the result is similar. The potential mechanism through which air pollution has an impact on perinatal mortality rates is infant weight. Conclusions: PM2.5 pollution has a significant and positive effect on perinatal mortality. The results show that environmental pollution control should be strengthened and the exposure of pregnant women in polluted air should be reduced.
Interstitial Lung Disease Associated Acute Respiratory Failure Requiring Invasive Mechanical Ventilation: A Retrospective Analysis
Authors: Vahdatpour Cyrus A. , Pichler Alexander , Palevsky Harold I , Kallan Michael J , Patel Namrata B , Kinniry Paul A 
Published: February 2020

Abstract
Background Interstitial lung disease (ILD) patients requiring invasive mechanical ventilation (IMV) for acute respiratory failure (ARF) are known to have a poor prognosis. Few studies have investigated determinants of outcomes and the utility of trialing non-invasive positive pressure ventilation (NIPPV) prior to IMV to see if there are any effect(s) on mortality or morbidity.Methods We designed a retrospective study using patients at four different intensive care units within one health care system. Our primary objective was to determine if there are differences in outcomes for in-hospital and one-year mortality between patients who undergo NIPPV prior to IMV and those who receive only IMV. A secondary objective was to identify potential determinants of outcomes.Results Of 54 ILD patients with ARF treated with IMV, 20 (37.0%) survived to hospital discharge and 10 (18.5%) were alive at one-year. There was no significant mortality difference between patients trialed on NIPPV prior to IMV and those receiving only IMV. Several key determinants of outcomes were identified with higher mortality, including: higher ventilatory support, idiopathic pulmonary fibrosis (IPF) subtype, high dose steroids, use of vasopressors, supraventricular tachycardias (SVTs), and higher body mass index.Conclusions Considering that patients trialed on NIPPV prior to IMV was associated with no mortality disadvantage to patients treated with only IMV, trialing patients on NIPPV may identify responders and avoid complications associated with IMV. Increased ventilator support, need of vasopressors, SVTs, and high dose steroids reflect higher mortality and palliative care involvement should be considered as early as possible if lung transplant is not an option.
TLR8 in the trigeminal ganglion contributes to the maintenance of trigeminal neuropathic pain
Authors: Zhao Lin-Xia , Bai Xue-Qiang , Cao De-Li , Wu Xiao-Bo , Jiang Ming , Zhang Jing , Guo Jian-Shuang , Chen Tong-Tong , Wu Hao , Gao Yong-Jing , Zhang Zhi-Jun 
Published: February 2020

Abstract
Background: Trigeminal neuropathic pain (TNP) is a significant health problem whereas the involved mechanism has not been completely elucidated. Toll-like receptors (TLRs) are recently demonstrated to be expressed in the dorsal root ganglion and involved in chronic pain. How TLR8 is expressed in the trigeminal ganglion (TG) after infraorbital nerve injury and whether TLR8 is involved in TNP have not been investigated.Methods: TNP model was established by the partial infraorbital nerve ligation (pIONL) in mice. The effect of TLR8 and its agonist VTX-2337 on pain hypersensitivity was checked by facial pain behavioral test. The immunostaining, real-time RT-PCR, and western blot were used to evaluate the expression of TLR8, pERK, pp38, and proinflammatory cytokines in the TG. The intracellular concentration of Ca 2+ was detected by the calcium imaging.Results: TLR8 was persistently increased in TG neurons in pIONL-induced TNP model. In addition, deletion of Tlr8 or knockdown of Tlr8 in the TG attenuated pIONL-induced mechanical allodynia, reduced the activation of ERK and p38, and decreased the expression of proinflammatory cytokines in the TG. Furthermore, intra-TG injection of TLR8 agonist VTX-2337 induced facial pain hypersensitivity. VTX-2337 also increased intracellular calcium concentration, induced activation of ERK and p38, and increased the proinflammatory cytokines expression in the TG.Conclusions: TLR8 contributes to the maintenance of TNP through increasing MAPK-mediated neuroinflammation. Targeting TLR8 signaling may be effective for the treatment of TNP.
Renal Hemangioblastoma with Mixed Mullerian tumor of endometrium: A tale of two rare primary tumors
Authors: Setia Aparna , Kumar Devender , Bains Lovenish , Sharma Pallavi , Tempe Anjali , Mallaya Varuna 
Published: February 2020

Abstract
Introduction Renal hemangioblastoma (RH) is a very rare benign tumor. Hemangioblastoma most commonly occur in the central nervous system (CNS) and only few cases of RH have been reported in literature. They occur most commonly as asymptomatic masses found incidentally. Mixed Mullerian Tumor (MMT) of the uterus is a rarer and aggressive form of uterine malignancy. The detection of two primary rare tumors incidentally is a rare entity. Case presentation A 50 years female patient presented with abnormal uterine bleeding which on endometrial sampling was diagnosed as a rare variety of endometrial cancer i.e., MMT or uterine carcinosarcoma. On preoperative Magnetic resonance imaging (MRI), a renal mass was also detected which was highly vascular and was mimicking Renal Cell Carcinoma (RCC). Fine needle aspiration cytology (FNAC) was done from the renal mass to differentiate between RCC and metastasis but it showed only blood cells. Patient underwent staging laparotomy for endometrial cancer and frozen section examination of the renal mass. Frozen section report was inconclusive with few atypical cells and thus patient underwent radical nephrectomy too. Histopathological examination revealed it to be a RH which is a very rare benign tumor. Discussion Only 14 cases of RH outside the CNS have been reported till date. Occurrence of renal mass as an incidental finding in the preoperative work up of uterine malignancy directed us to the differentials of metastasis or another histologically distinct primary tumor. RH may mimic RCC radiologically. To correctly diagnose RH is important because sporadic RH does not require further treatment and the prognosis is much better than that of RCC. MMT which compromise 1-2% of all uterine neoplasms have higher chance of metastasis than endometroid variety. Conclusion Renal hemangioblastoma is a rare benign tumor which doesn’t require any treatment in majority of the patients. Mixed Mullerian tumor is a rare aggressive uterine tumor which metastasizes early, henceforth early identification and treatment is the key. RH needs to be differentiated from RCC to avoid over treatment. Morphological findings are similar in both, preoperative FNAC, PET scan and intraoperative frozen section can be utilized to differentiate in between two in well circumcised tumors and in high suspicion. The presence of two rare primary tumors i.e., RH and MMT in the same patient which are unrelated is a rare entity.
Reclassification of tumor size for solitary HBV-related hepatocellular carcinoma by minimum p value method: a large retrospective study
Authors: Liu Hongzhi , Yang Yuan , Chen Chuanchun , Wang Lei , Huang Qizhen , Zeng Jianxing , Lin Kongying , Zeng Yongyi , Guo Pengfei , Zhou Weiping , Liu Jingfeng 
Published: February 2020

Abstract
Background and Objectives Tumor size is one of the most important issues for hepatocellular carcinoma (HCC) treatment and prognosis but the classification of it still controversial. The aim of this study was to screen appropriate cutoffs for size of solitary HBV-related HCC. Methods A cohort of 1760 patients with solitary HBV-related HCC undergoing curative liver resection were divided into 11 groups based on tumor size in 1-cm intervals. The minimum p value method was used to screen the appropriate size cutoff according to overall survival (OS). Results There are 8 dichotomy, 8 trichotomy and 14 inquartation cutoffs were screened when classifying tumor sizes in accordance with OS. After reclassified patients into three new classifications: ≤ 3cm (n = 422), > 3 and ≤ 9cm (n = 1072), and > 9cm (n = 266). The comparison of clinicopathologic characteristics among these three classifications showed that the increase of tumor size was associated with the increase of α-fetoprotein (AFP), Microvascular invasion (MVI), tumor differentiation, and liver cirrhosis. And the comparison of the OS among three classifications showed statistical differences. Conclusions This study suggested that size criteria of 3cm and 9cm in solitary HBV-related HCC patients were appropriate based on biological characteristics and prognostic significance.