bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort
Authors: Chen Yulian , Qiu Xingdi , Wang Wenjing , Li Dong , Wu Anyue , Hong Zubei , Di Wen , Qiu Lihua 
Published: August 2020

Abstract
Background: In this study, the association between human papillomavirus (HPV) infection and related cervical intraepithelial neoplasia (CIN) or cervical cancer and vaginal microbiome was evaluated in Chinese cohorts. Methods: The vaginal bacterial composition of five groups, HPV-infected women without CINs (HPV, n=78), women with low-grade squamous intraepithelial lesions (LSIL, n=51), women with high-grade squamous intraepithelial lesions (HSIL, n=23), women with invasive cervical cancer (Cancer, n=9) and healthy women without HPV infection (Normal, n=68), was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V3-4) using Illumina MiSeq. Results: HPV infection increased vaginal bacterial richness and diversity regardless of the status of CINs. The vaginal bacterial richness and diversity were further augmented in women with cervical cancer. Lactobacillus was the most abundant genus in all groups. HPV infection had a negative influence on the abundances of Lactobacillus, Gardnerella and Atopobium. Accordingly, HPV infection increased the relative abundance of Prevotella, Bacillus, Anaerococcus, Sneathia, Megasphaera, Streptococcus and Anaerococcus. The increased proportions of Bacillus, Anaerococcus and the reduced abundance of Gradnerella vaginalis were probably related with the progression of CINs severity. HPV infection without CINs or cancerous lesions was strongly associated with Megasphaera. The most abundant bacterium in the LSIL group was Prevotella amnii. However, Prevotella timonensis, Shuttleworthia and Streptococcaceae at the family level were three taxa related to HSIL. Furthermore, more taxa were associated with the Cancer group including Bacillus, Sneathia, Acidovorax, Oceanobacillus profundus, Fusobacterium, Veillonellaceae at the family level, Anaerococcus and Porphyromonas uenonis. Samples in the Normal group were mostly assigned to CST III. HPV infection converted the vaginal bacterial community structure from CST III to CST IV. Furthermore, the proportions of CST IV were gradually augmented with the progression of the severity of CINs.Conclusions: This work interpreted the differential vaginal bacteria under HPV infection and various precancerous or cancerous lesions in a Chinese cohort. We distinguished the specific microbes and the vaginal bacterial structure that were related with the progression of CINs severity in Chinese women.
Surgical management of consecutive multisegment thoracic and lumbar tuberculosis: anterior-only approach vs. posterior-only approach.
Authors: Zhao Chen , Luo Lei , Liu Liehua , Li Pei , Liang Lichuan , Gao Yongjian , Luo Fei , Xu Jianzhong , Zhou Qiang 
Published: August 2020

Abstract
Purpose: To compare the efficacy, safety, and technical characteristics of anterior-only and posterior-only approach surgeries for the treatment of consecutive multisegment thoracic and lumbar tuberculosis . Methods: Thirty-five patients who developed consecutive multisegment thoracic and lumbar tuberculosis from September 2012 to May 2016 were retrospectively analyzed. Group A was the posterior-only surgery group, and group B was the anterior-only surgery group. The data on the surgery, deformity correction, functional scores and complications were compared between the two groups. Results: There was no significant difference in the operation time or blood loss between groups A and B (P>0.05). The preoperative average Cobb angle of kyphosis in groups A and B were 36.2±15.2° and 27.9±7.7°, respectively, which significantly decreased to 4.9±11.8° and 10.4±5.6° after the operation, respectively (P<0.05). At the final follow-up, the angles were 7.1±10.5° and 14.6±8.0°, respectively. The correction angle and correction rate in group A (31.3±16.6°, 88.6±43.6%) were greater than those in group B (17.5±4.4°, 64.9±14.0%) (P<0.05). There was no significant difference in the loss angle between groups A and B (P>0.05), but the loss rate in group B (24.0±27.8%) was higher than that in group A (9.6±10.2%) (P<0.05). There was no significant difference in the incidence of complications between the two groups (P>0.05). Conclusion: The posterior-only and anterior-only approaches can lead to satisfactory clinical results in the treatment of patients with consecutive multisegment thoracic and lumbar tuberculosis. With posterior-only surgery, kyphosis can be better corrected, and the correction can be better maintained than with anterior-only surgery.
Interleukin-8 is not a predictive biomarker for the development of the acute promyelocytic leukemia differentiation syndrome
Authors: de Almeida Luciana Yamamoto , Pereira-Martins Diego Antonio , Lima Ana Sílvia Gouvêa , Baggio Márcia Sueli , Koury Luisa Corrêa de Araujo , Lange Ana Paula , Bassi Sarah Cristina , Scheucher Priscila Santos , Rego Eduardo 
Published: August 2020

Abstract
Background

Differentiation syndrome (DS) is the main life-threatening adverse event that occurs in acute promyelocytic leukemia (APL) patients treated with all-trans retinoic acid (ATRA). Cytokine imbalances have been reported to play role during the developing of acute promyelocytic leukemia differentiation syndrome (APL-DS). However, the relationship between the plasma cytokine levels and their prognostic value for the prediction of DS developing in patients with APL during the treatment with ATRA and anthracyclines has not been previously reported.

Methods

In this study, we followed an APL cohort (n=17) over seven days of ATRA therapy in DS (n=6) and non-DS groups (n=11). Interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70 and TNF-α were measured in the peripheral blood plasma from 17 patients with APL and 11 healthy adult controls by using the cytometric bead array method.

Results

In non-DS patients, IL-8 plasma levels were significantly reduced in the seventh day of ATRA treatment (34.16; 6.99 to 147.11 pg mL -1 in D0 vs. 10.9; 0 to 26.81 pg mL -1 in D7; p = 0.02) whereas their levels did not discriminate between DS and non-DS development during the entire induction period (all p > 0.05 in D0, D3, and D7). No significant differences were found in IL-6 levels between groups ( p > 0.05 in D0-D7). Other cytokines tested were all undetectable in patients with APL or healthy controls.

Conclusions

We demonstrated that the modulation of IL-8 following ATRA treatment may occur regardless of the development of DS and, therefore, does not appear to be a predictive biomarker to monitor the APL-DS.
Fertility and pregnancy issues in a 46, XY (Swyer syndrome) patient in the face of ovarian cancer
Authors: Olszak-Wąsik Katarzyna , Tukiendorf Andrzej , Stanek-Widera Agata , Olejek Anita 
Published: August 2020

Abstract
Background In this paper we focus on 46, XY pure gonadal dysgenesis-Swyer syndrome. MethodsWe present the case of a 33-year old patient and the medical procedures enabling to achieve a pregnancy in the couple struggling with infertility.We analyzed patient’s medical history, scheduled further diagnostic and therapeutic procedures that included genetic tests and consultations, MRI (magnetic resonance imaging), laparoscopy, oncological risk assesment and possibilities of pregnancy.Results Patients medical history revealed her last menstrual period dated one year before she started searching for medical help. Due to a low AMH (anti-Muellerian hormone) level, genetic consultation and genetic tests were recommended. The patient was diagnosed with Swyer syndrome. She underwent laparoscopy to search for gonadal tissue. Histopathology of removed streak tissues revealed dysgerminoma. No metastases were found in a follow up MRI. The patient decided to participate in an egg donation program.Conclusions The diagnosis of karyotype containing Y chromosome in a phenotypical adult female patient raised as female demands optimal managing. It has to cover not only psychological and fertility aspects, but also the risk of development of gonadal tumors.
Effect of meteorological factors on the activity of influenza in Chongqing, China, 2012–2019
Authors: Qi Li , Liu Tian , Gao Yuan , Tian Dechao , Li Qin , Su Kun , Tang Wenge , Feng Luzhao , Liu Qiyong 
Published: August 2020

Abstract
Background: The effects of multiple meteorological factors on influenza activity remain unclear in Chongqing, the largest municipality in China. We aimed to fix this gap in this study.Methods: Weekly meteorological data and influenza surveillance data in Chongqing were collected from 2012 to 2019. Distributed lag nonlinear models (DLNMs) were conducted to estimate the effects of multiple meteorological factors on influenza activity.Results: Inverted J-shaped nonlinear associations between mean temperature, wind speed, sunshine and influenza activity were found. The relative risks (RRs) of influenza activity increased as weekly average mean temperature fell below 18.18℃, average wind speed fell below 1.55 m/s and average sunshine fell below 2.36 hours. Taking the median values as the references, lower temperature and windless could significantly increase the risks of influenza activity and last for 4 weeks. A J-shaped nonlinear association was observed between relative humidity and influenza activity; the risk of influenza activity increased with rising relative humidity with 78.26% as the break point. Taking the median value as the reference, high relative humidity could increase the risk of influenza activity and last for 3 weeks. In addition, we found the relationship between aggregate rainfall and influenza activity could be described with a U-shaped curve. Rainfall effect has significantly higher RR than rainless effect.Conclusions: Our study shows that multiple meteorological factors have strong associations with influenza activity in Chongqing, providing evidence for developing a meteorology-based early warning system for influenza to facilitate timely response to upsurge of influenza activity.
Parents’ Perceived Mental Distress When Their Infant is Admitted to a Neonatal Intensive Care Unit (NICU) a Quantitative Cross-Sectional Study
Authors: Hagen Inger Hilde , Svindseth Marit Føllsvik , Vasset Frøydis Perny 
Published: August 2020

Abstract
Background. It is normal for parents to experience a range of emotions and changes in behaviour while their newborn infant is in the NICU. The stress experienced by these parents during their infant’s hospital stay is found to affect the parent-infant relationship and their ability to bond reciprocally. Several studies have pointed out that support from family and friends can be useful for parents’ mental distress in this situation. Some differences are found between mothers’ and fathers’ experiences of the support. The aim of the current study is to examine parents’ perceived mental distress when their infant is admitted to a neonatal intensive care unit (NICU) and factors important for their support. The relationship between parents perceived mental distress and satisfaction with NICU care are spare researched. Methods. A multicentre prospective cohort study. A total of 568 parental participants from six different NICUs geographically spread throughout Norway. Hospital Anxiety and Depression Scale (HADS) and Impact of Events Scale-Revised (IES-6) were used to collect data. All the responses were rated and analysed using parametric analysis methods, descriptive statistics, logistic regression, factor analyses and linear regression analyses.Results. A total of 275 mothers reported receiving support from family and friends compared with 232 fathers. Younger parents had a higher OR of reporting anxiety compared with that of older parents. Parents with a primary or high school education level showed a higher OR of satisfaction than did parents with a college or university education level.Conclusion. There are differences in mothers’ and fathers’ responses to their experiences of various distress symptoms related to their children being in the NICU, as well as their received support from family and friends. There are also outcome differences between the age and education level of the parents and their experience of stress in the NICU. Mothers are more distressed and have more anxiety and depression than do fathers have.Trail registration: This project was first presented to the Regional Committees for Medical and Health Research Ethics which reported that the project was outside its mandate (2015/386). The project is approved from the Norwegian Data Protection Officials.
Factors Associated with Blood Pressure Checkup During Pregnancy Among Women of Reproductive Age in Tanzania; An Analysis of Data from the 2015-16 Tanzania Demographic and Health Survey and Malaria Indicators Survey
Authors: Moshi Fabiola Vincent , Tungaraza Maximilian 
Published: August 2020

Abstract
Background: Hypertensive Disorder of Pregnancy (HDP) is one of the leading causes of maternal mortality and morbidity amongst pregnant women in the world. Blood pressure check-ups during pregnancy constitute one of the strategies used to identify hypertensive disorders, hence timely management. Little is known about the factors associated with blood pressure check-ups in Tanzania.Method: The study used data from 2015 - 16 Tanzania Demographic and Health Survey and Malaria Indicators Survey (2015 - 16 TDHS - MIS). A total of 6924 women of active reproductive age from 15 to 49 were included in the analysis. Both univariate and multiple regression analyses were used to determine the association between early antenatal booking and maternal services utilization.Results: A total of 4997(72.17%) interviewed women were checked for blood pressure at least once. Having been adjusted for the confounders, the factors which influenced an uptake of blood pressure check-ups during pregnancy were timed for antenatal booking within first 12 weeks, AOR=1.496 at 95% CI= 1.297-1.726, p<0.001, age group [more than 34 years, (AOR=1.518 at 95% CI=1.149-2.006, p=0.003)], wealth index [middle income, (AOR=1.215 at 95% CI=1.053-1.468, p=0.008) and rich, (AOR=2.270 at 95% CI=1.907-2.702, p<0.001)] reference population being poor; education level [primary education, (AOR=1.275 at 95% CI=1.107-1.468, p=0.001); secondary education, (AOR=2.163 at 95% CI=1.688-2.774, p<0.001) and higher education, (AOR=9.929 at 95%CI=1.355-72.76, p=0.024)] reference population being no formal education; parity [para 2-4, (AOR=1.190 at 95% CI=1.003-1.412, p=0.046) and zones [Unguja Island, (AOR=3.934 at 95% CI=1.568-9.871, p=0.004), Pemba Island, (AOR=5.308 at 95%CI=1.808-15.58, p=0.002)] and Mainland Urban being the reference population.Conclusion: Blood pressure check-ups during pregnancy offer the opportunity for early detection, hence timely management of HDP. The study revealed that rural dwelling pregnant women had higher chance of not getting their BP checked. It was also revealed that maternal age, education level, place of residence, wealth index and timing of ANC services were significantly associated with blood pressure check-ups. The study recommends the need to explore significant factors associated with utilization of available free reproductive health services across all public health facilities. It also recommends the need to address prioritized intensive awareness programs and behavioral change interventions on the significance of BP check-ups among pregnant women of reproductive age.
Neuroprotective Effects of microRNA-140-5p on Ischemic Stroke in Mice via its Regulation on the TLR4/NF-κB Axis
Authors: Wenjun Song , Wang Tiancheng , Shi Bei , Wu Zhijun , Wang Wenjie , Yang Yanhong 
Published: August 2020

Abstract
Background and aim: Ischemic stroke is one of the main causes of death worldwide and permanent global disability. On the basis of existing literature data, we carried out studies in an effort to explore how miR-140-5p affects ischemic stroke and whether the mechanism relates to toll-like receptor-4 (TLR4) and nuclear factor-kappa B (NF-κB).Methods: Middle cerebral artery occlusion (MCAO) was employed to establish a mouse model of ischemic stroke in vivo, while primary neurons were exposed to oxygen-glucose deprivation (OGD) to set up an ischemic stroke model in vitro. RT-qPCR was performed to detect the miR-140-5p expression and Western blot was employed to detect the expression TLR4, NF-κB, and apoptosis-related factors. Then, based gain-function of experiments using miR-140-5p mimic and TLR4 overexpression plasmid, neurological function score, TTC staining, TUNEL staining, as well as flow cytometry were carried out to evaluate the effects of miR-140-5p and TLR4 on MCAO mice and OGD neurons. Meanwhile, dual-luciferase reporter assay was used to validate the relationship between miR-140-5p and TLR4.Results: miR-140-5p expressed at a low level and TLR4 at a high level in ischemic stroke. It was verified that miR-140-5p targeted TLR4 and downregulated its expression. miR-140-5p overexpression was observed to inhibit the apoptosis of neurons under OGD exposure and restrain the progression of ischemic stroke, while TLR4 overexpression promoted the apoptosis and disease progression. Besides, miR-140-5p overexpression led to a decrease in NF-κB protein level, which was increased by TLR4 overexpression. Conclusion: In conclusion, from our data we conclude that miR-140-5p overexpression may be instrumental for the therapeutic targeting of ischemic stroke by alleviating neuron injury with the involvement of TLR4/NF-κB axis.
Transradial Versus Transfemoral Access for Cardiac Catheterization: A Nationwide Pilot Study of Training Preferences and Expertise in USA.
Authors: Changal Khalid Hamid , Syed Mubbasher Ameer , Atari Ealla , Nazir Salik , Saleem Sameer , Gul Sajjad , Salman FNU , Inayat Asad , Eltahawy Ehab 
Published: August 2020

Abstract
Background: The objective was to assess current training preferences, expertise, and comfort with transfemoral access (TFA) and transradial access (TRA) amongst cardiovascular training fellows and teaching faculty in United States. As TRA continues to dominate the field of interventional cardiology, there is a concern that trainees may become less proficient with the femoral approach. Methods: A detailed questionnaire was sent out to academic General Cardiovascular and Interventional Cardiology training programs in the United states. Responses were sought from fellows-in-training and faculty regarding preferences and practice of TFA and TRA. Answers were analyzed for significant differences between trainees and trainers using the Pearson χ2 or Fisher’s exact test, or the Student t test or the Mann–Whitney U test. Results: A total of 125 respondents (75 fellows-in-traing and 50 faculty) completed and returned the survey. The average grade of comfort for TFA, on a scale of 0 to 10 (10 being most comfortable), was reported to be 6 by fellows-in-training and 10 by teaching faculty (p < 0.001). TRA was the first preference in 95% of the fellows-in-training compared to 69% of teaching faculty (p 0.001). While 62% of fellows believed that they would receive the same level of training as their trainers by the time they graduate, only 35% of their trainers believed so (p 0.004).Conclusion: The shift from TFA to radial first has resulted in significant concern among cardiovascular fellows-in training and the faculty regarding training in TFA. Cardiovascular training programs must be cognizant of this issue and should devise methods to assure optimal training of fellows in gaining TFA and managing femoral access-related complications.
Improving CLIP-Seq Data Analysis by Incorporating Transcript Information
Authors: Uhl Michael , Tran Dinh Van , Backofen Rolf 
Published: August 2020

Abstract
Background: Current peak callers for identifying RNA-binding protein (RBP) binding sites from CLIP-seq data take into account genomic read profiles, but they ignore the underlying transcript information, that is information regarding splicing events. So far, there are no studies available that closer observe this issue. Results: Here we show that current peak callers are susceptible to false peak calling near exon borders. We further quantify its extent in publicly available datasets, which turns out to be substantial. Finally, by providing a tool called CLIPcontext for automatic transcript and genomic context sequence extraction, we demonstrate that context choice also affects the performances of RBP binding site prediction tools. Conclusions: Our results demonstrate the importance of incorporating transcript information in CLIP-seq data analysis. Taking advantage of the underlying transcript information should therefore become an integral part of future peak calling and downstream analysis tools.
PacBio full-length transcriptome of wild apple (Malus sieversii) provides insights into canker disease dynamic response
Authors: Liu Xiaojie , Li Xiaoshuang , Wen Xuejing , Zhang Yan , Ding Yu , Zhang Yiheng , Gao Bei , Zhang Daoyuan 
Published: August 2020

Abstract
Background
Cytospora canker is a serious disease in the stem of Malus sieversii, caused by Cytospora mali. However, little is known about the global response mechanism in M. sieversii to C. mali infection.
Results
Phytohormone jasmonic acid (JA) and salicylic acid (SA) profiles and transcriptome analysis were used to elaborate on the dynamic response mechanism. We determined that the JA was initially produced to respond to the necrotrophic pathogen C. mali infection at the early response stage, then get synergistically transduced with SA to respond at the late response stage. Furthermore, we adopted Pacific Biosciences (PacBio) full-length sequencing to identify differentially expressed transcripts (DETs) during the canker response stage. We obtained 52,538 full-length transcripts, of which 8,139 were DETs. Total 1,336 lncRNAs, 23,737 alternative polyadenylation (APA) sites and 3,780 putative transcription factors (TFs) were identified. Additionally, functional annotation analysis of DETs indicated that the wild apple response to the infection of C. mali involves plant-pathogen interaction, plant hormone signal transduction, flavonoid biosynthesis, and phenylpropanoid biosynthesis. The co-expression network of the differentially expressed TFs revealed 264 candidate TF transcripts. Among these candidates, the WRKY family was the most abundant. The MsWRKY7 and MsWRKY33 were highly correlated at the early response stage, and MsWRKY6, MsWRKY7, MsWRKY19, MsWRKY33, MsWRKY40, MsWRKY45, MsWRKY51, MsWRKY61, MsWRKY75 were highly correlated at the late stage.
Conclusions
The full-length transcriptomic analysis revealed a series of immune responsive events in M. sieversii in response to C. mali infection. The phytohormone signal pathway regulatory played an important role in the response stage. Additionally, the enriched disease resistance pathways and differentially expressed TFs dynamics collectively contributed to the immune response. This study provides valuable insights into a dynamic response in M. sieversii upon the necrotrophic pathogen C. mali infection, facilitates understanding of response mechanisms to canker disease for apple, and provides supports in the identification of potential resistance genes in M. sieversii.