bluuebunny/crossref_metadata_2025_split huggingface dataset:
Periodontal Treatment by Dentistry Undergraduate Students. Assessment of the Patient's Oral Quality of Life: A Prospective Pilot Study.
Authors: Azaustre María Paloma Alvarez , Bravo Manuel , Magan-Fernandez Antonio , Rodríguez-Archilla Alberto , Llena Carmen , Mesa Francisco 
Published: September 2020

Abstract
Background: Our aim was to assess the impact of non-surgical periodontal treatment on oral health-related quality of life of patients with periodontitis, performed by undergraduate dental students. Methods: An observational prospective one cohort study pre‑post test was performed, involving 31 undergraduate dental students. A complete periodontal examination was performed before and after receiving non-surgical periodontal treatment. The main independent clinical variables assessed were the grade of periodontal inflammation and the number of teeth with periodontitis. Oral health-related quality of life was assessed before and after treatment through the Oral Impacts on Daily Performances (OIDP) questionnaire. The association between the extension of periodontal treatment (measured as number of treated teeth) and final OIDP score was assessed, adjusting for age, sex and baseline OIDP, in a multiple linear regression model. Results: 34 patients were enrolled and treated by the undergraduate students. The global absolute score, the mean OIDP value, was reduced from 26.2 to 12. The global percent score, percentage of impact, was reduced from 13 to 6. Non-surgical periodontal treatment showed an improvement in oral health-related quality of life. However, no association between the number of treated teeth and none of the aspects measured by ODIP was observed post-treatment, adjusting for age, sex and baseline OIDP. Conclusion: Non-surgical periodontal treatment performed by undergraduate dental students on periodontal patients, did not show any impact on oral health-related quality of life, measured by OIDP.
Insights into the Therapeutic of Glucocorticoid for Refractory Mycoplasma Pneumoniae Pneumonia in Children
Authors: Zhu Zhenli , Zhang Tongqiang , Guo Wei , Ling Yaoyao , Tian Jiao , Xu Yongsheng 
Published: September 2020

Abstract
Objective To observe the efficacy and safety of different doses of glucocorticoid for refractory mycoplasma pneumoniae pneumonia in children, analyze the clinical characteristics in different groups of patients, and explore the factors related to affect illness severity for children with refractory mycoplasma pneumoniae pneumonia and guide the dosage of glucocorticoids.Methods Retrospective analysis was performed on 279 children with refractory mycoplasma pneumoniae pneumonia hospitalized in our hospital between September 2018 and October 2019. 23 children were excluded, the remaining 256 children were divided into three subgroups: Group I was not given methylprednisolone (n=75), group II (n=115) was given methylprednisolone ≤125mg/d, and group III was given methylprednisolone >125mg/d (n=66). The clinical features, laboratory data, radiological manifestations between three subgroups of children were compared, relevant indicators with meaningful were used for ROC curve and multiple logistic regression analysis, and the optimal values of related factors were analyzed.Results The median age and median weight of the group III were greater than the group II（P <0.05）, the median age and median weight of the group I were greater than the group II（P <0.05）, there was no statistical significance in median age and median weight between group III and group I（P>0.05）. The group II is more serious than that of group I, and group III is more serious than that of group II, higher incidence of hypoxemia, longer fever, longer hospital stays, higher incidence of extrapulmonary complications, and more severe of radiological findings (P <0.05). The more severe presentation of disease, hormones dosage was larger, the use rate of gamma globulin was higher, the use rate of bronchoscopy was higher, and higher incidence of plastic bronchitis (P <0.05). Meanwhile, WBC, CRP, LDH, FER, D-D dimer, APTT, PLT, PCT, IL-6, ALT and the percentage of neutrophils in the three groups showed a gradual upward trend (P <0.05). In ROC curve analysis, WBC, neutrophils percentage, CRP, LDH, Fer, PCT and IL-6 can be used to distinguish RMPP with different severity and to guide the dosage of glucocorticoids. Multivariate logistic regression analysis showed that LDH 424.5IU/L, PCT 0.145ng/ml, IL-6 26.69pg/ml and lung consolidation were significant predictors for the severity of RMPP and glucocorticoids dose.Conclusions LDH 424.5IU/L, PCT 0.145 ng/ml, IL-6 26.69pg/ml and pulmonary consolidation as markers of disease severity in patients with RMPP and the dosage of glucocorticoids, which can aid in early recognition of children with severe illness, use appropriate doses of hormones, and reduce sequelae.
Development of a Fixed Repertoire of Blood Transcriptome Modules Based on Co-expression Patterns Across Immunological States
Authors: Altman Matthew , Rinchai Darawan , Baldwin Nicole , Toufiq Mohammed , Whalen Elizabeth , Garand Mathieu , Kabeer Basirudeen , Alfaki Mohamed , Presnell Scott , Khaenam Prasong , Ayllon-Benitez Aaron , Mougin Fleur , Thébault Patricia , chiche Laurent , Jourde-Chiche Noemie , Phillips Theodore , Klintmalm Goran , O'Garra Anne , Berry Matthew , Bloom Chloe , Wilkinson Robert , Graham Christine , Lipman Marc , Lertmemongkolchai Ganjana , Bedognetti Davide , Thiébaut Rodolphe , Kheradmand Farrah , Mejias Asuncion , Ramilo Octavio , Palucka Karolina , Pascual Virginia , Banchereau Jacques , Chaussabel Damien 
Published: September 2020

Abstract
As the capacity for generating large scale data continues to grow the ability to extract meaningful biological knowledge from it remains a limitation. Here we describe the development of a new fixed repertoire of transcriptional modules. It is meant to serve as a stable reusable framework for the analysis and interpretation of blood transcriptome profiling data. It is supported by customized resources, which include analysis workflows, fingerprint grid plots data visualizations, interactive web applications. These provide access to a vast number of module-specific functional profiling reports, reference transcriptional profiles, and give users the ability to visualize changes in transcript abundance across the modular repertoire at different granularity levels. A use case focusing on a set of six modules comprising interferon-inducible genes is also provided. Taken together, this well-characterized set of modules may be employed for the interpretation and benchmarking of blood transcriptome profiles obtained within and across patient cohorts.
Analysing the Effectiveness of Topical Bleeding Care Following Tooth Extraction in Patients Receiving Dual Antiplatelet Therapy. Personal Experiences.
Authors: Lewandowski Bogumił , Myszka Aleksander , Migut Małgorzata , Czenczek-Lewandowska Ewelina , Brodowski Robert 
Published: September 2020

Abstract
Background Patients using antiplatelet drugs following infarctions, acute coronary syndrome (ACS) or stroke pose a significant clinical problem if it is necessary to perform surgery, including dental surgery, since they are at risk of prolonged or secondary post-extraction bleeding. Discontinuation of this therapy is associated with a high risk of serious thromboembolic complications. The purpose of this study was to assess the effectiveness of TachoSil fibrin-collagen patches in stopping and preventing of secondary post-extraction bleeding in patients undergoing chronic antiplatelet therapy.Methods The study was conducted through retrospective examination of the medical records of 153 patients using chronic antiplatelet therapy and those qualified for tooth extraction. The largest group comprised 74 patients using aspirin and clopidogrel as dual platelet antiaggregation therapy; in this group 75 tooth extractions were carried out. In all of the patients TachoSil fibrin-collagen patches and stiches were applied to the wounds resulting from tooth removal. Results Following tooth extraction, primary bleeding was stopped in all the patients and their wounds closed via coagulation within 20-30 minutes. In eight cases, accounting for 4.9% of the patients, secondary bleeding occurred and was successfully stopped only by applying a pressure dressing soaked in tranexamic acid. Secondary bleeding occurred in three patients on the second day and in five patients on the third day following tooth removal. Conclusion Topical application of TachoSil patches following tooth removal in patients using single or dual antiplatelet therapy effectively stopped bleeding and prevented secondary bleeding after tooth extraction.
Computational Barthel Index: An Automated Tool for Assessing and Predicting Activities of Daily Living Among Nursing Home Patients
Authors: Wojtusiak Janusz , Asadzadehzanjani Negin , Levy Cari , Alemi Farrokh , Williams Allison E. 
Published: September 2020

Abstract
Background: Assessment of functional ability, including Activities of Daily Living (ADLs), is a manual process completed by skilled health professionals. We investigated the possibility of constructing an automated decision support tool, the Computational Barthel Index Tool (CBIT), that automatically assesses and predicts probabilities of current and future ADLs based on patients’ medical history. Methods: The data used to construct the tool include the demographic information, diagnosis codes, and reported disabilities of 181,213 residents of the Department of Veterans Affairs’ (VA) Community Living Centers. Supervised machine learning methods were applied to construct the CBIT. Temporal information about times from the first and the most recent occurrence of diagnoses was encoded. Ten-fold cross-validation was used to tune hyperparameters, and independent test sets were used to evaluate models using AUC, accuracy, recall, and precision. Random forest achieved the best model quality. Models were calibrated using isonomic regression. Results: The unabridged version of CBIT uses 578 patient characteristics and achieved average AUC of 0.94 (0.93-0.95), accuracy of 0.90 (0.89-0.91), precision of 0.91 (0.89-0.92), and recall of 0.90 (0.84-0.95) when re-evaluating patients. CBIT is also capable of predicting ADLs up to one year ahead, with accuracy decreasing over time, giving average AUC of 0.77 (0.73-0.79), accuracy of 0.73 (0.69-0.80), precision of 0.74 (0.66-0.81), and recall of 0.69 (0.34-0.96). A simplified version of CBIT with 50 top patient characteristics reached performance that does not significantly differ from full CBIT.Conclusion: Discharge planners, disability application reviewers, clinicians evaluating comparative effectiveness of treatments can use CBIT to assess and predict information on functional status of patients.
Identification and Functional Analysis of A Non-Mammalian IRF10 in Common Carp (Cyprinus Carpio. L) in The Antiviral and Antibacterial Immune Response
Authors: Zhu Yaoyao , Shan Shijuan , Zhao Huaping , Liu Rongrong , Wang Hui , Chen Xinping , Yang Guiwen , Li Hua 
Published: September 2020

Abstract
Background: Interferon (IFN) regulatory factors (IRFs), as the transcriptional regulatory factors play important roles in regulating the expression of type I IFN and IFN-stimulated genes (ISGs) in innate immune responses, as well as participate in cell growth, development and regulating the oncogenesis.Results: In the present study, the cDNA sequence of IRF10 was characterized in common carp (Cyprinus carpio L.) (abbreviation, CcIRF10). The predicted protein sequence of CcIRF10 shared 52.7-89.2% identities to teleost IRF10 and contained a DNA-binding domain (DBD), a nuclear localization signal (NLS) and an IRF associated domain (IAD). Phylogenetic analysis showed that CcIRF10 had a closer relationship with the IRF10 of Ctenopharyngodon idella. CcIRF10 transcripts was detectable in all examined tissues, with the highest expression in the gonad and the lowest expression level in the head kidney. The CcIRF10 expression was up-regulated in the spleen, head kidney, foregut and hindgut upon polyinosinic: polycytidylic acid (poly I:C) and Aeromonas hydrophila stimulation, and also induced by poly I:C, LPS and PGN in the peripheral blood leucocytes (PBLs) and head kidney leukocytes (HKLs) of C. carpio. In addition, overexpression of CcIRF10 was able to decrease the expressions of IFN-related genes, PKR and ISG5.Conclusions: These results indicated that CcIRF10 participated in the antiviral and antibacterial immunity, and negatively regulated the IFN response, which provided new insights into the IFN system of C. carpio.
Microbial Profile of Root Canals of Pulpally Infected Teeth In Ghanaians
Authors: Konadu Akua Boakyewaa , Nyako Ebenezer Anno , Ampofo Patrick Caldicock , Akyeh Moses Lorenzo , Ndanu Thomas Akuetteh , Yeboah-Manu Dorothy 
Published: September 2020

Abstract
Background Pulpal and periapical infections are initiated by microorganisms when they gain access into the dental pulp. The success of root canal treatment principally depends on the eradication of the micro-organisms in the root canal system. The aim of the study was to determine the microbial agents of infected root canals in Ghanaians patients. Methodology Forty four consecutive patients with sixty teeth referred to the Restorative Dentistry Clinic requiring root canal treatment were recruited. Root canal samples were collected from the teeth with sterile paper points. The samples were processed at the laboratory setup created at the chairside, subjected for microbial analysis and identification using Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS).ResultsA total of 259 isolates were recovered from the 60 infected root canals, belonging to twenty (20) different microbial genera. Out of the 259 microbial species isolated, only two (2) were candida albicans, a fungi; 257(99.2%) were bacterial isolates belonging to 19 genera. The 19 genera had 53 bacterial species, out of which 26(49.1%) were identified as facultative anaerobes, 15(28.3 %.) as obligate anaerobes and 12(22.6%) were aerobes. Streptococcus species (Streptococcus oralis, S.mitis, S. mutans and S. constellatus) were the most predominant isolates, followed by Prevotella sp, Actinomyces sp, Enterococcus faecalis and Rothia sp respectively. ConclusionThe findings of this study show that, primary root canal infections are polymicrobial with facultative anaerobes been predominant. The determination of the microbial profile aids in understanding the pathogenesis of pulpal and periradicular infections and help in choosing effective antimicrobial irrigation and medicament for root canal treatment.
Prognostic value of circulating plasma cells in newly diagnosed multiple myeloma
Authors: Han Wenmin , Jin Yuanyuan , Xu Min , Zhao Sishu , Shi Qinglin , Qu Xiaoyan , Zhang Run , Li Jianyong , Wu Yujie , Chen Lijuan 
Published: September 2020

Abstract
Purpose: Multiple myeloma (MM) is a clinically and biologically heterogeneous malignancy of plasma cell. The overall survival of MM patients varies from people to people ranged from several months to decades. It is always knotty how to predict MM prognosis. The presence of circulating plasma cells (CPCs) has been associated with a worse prognosis in patients with MM.Materials and Methods: This study retrospectively analyzed CPCs in 108 cases of newly diagnosed MM patients with 8-color flow cytometry to investigate its value for outcome prediction, and combined CPCs with R-ISS to stratify the risk of MM. Results: CPCs were detected in 58/108 patients (53.7%). The optimum cutoff predicting for overall survival was determined as 0.105% by using a ROC analysis. Compared with patients with CPCs < 0.105% (n = 66，61.1%), those with CPCs ≥0.105% (n = 42, 38.9%) showed lower blood platelet count (BPC) (P=0.038), but higher β2-microglobulin (β2-MG), lactate dehydrogenase (LDH), ferritin (FER) , and harboring P53 deletion, high-risk cytogenetic abnormality, (P = 0.011, 0.001, 0.002, <0.001, and 0.020, respectively). The higher R-ISS stage seems to harbor higher CPCs. CPCs≥0.105% are independently factor for adverse outcome (P<0.001). The combination of R-ISS staging system and CPCs level was used to stratify the risk of multiple myeloma，and R-ISS III stage with CPCs ≥0.105% was ranked as a real ultra-high-risk group. Conclusion: This study suggests that high CPCs is associated with an aggressive disease and that the current R-ISS system in conjunction with CPCs may facilitate the differentiation of NDMM patients. There may also is the potential significance in modifying the definitions of high-risk disease and the practice of adopting a risk-adapted initial treatment.
Sniffer Dogs as a Screening/Diagnostic Tool for COVID-19, A Proof of Concept Study
Authors: Eskandari Esmaeil , Shiri Mahdi , Aliyazdi Helen , Farahani Ramin Hamidi , Nezami-Asl Amir , Laripour Reza , Roudgari Hassan , Moghaddam Arasb Dabbagh , Zibaseresht Ramin , Marzaleh Milad Ahmadi , Akbarialiabad Hossein , Zoshk Mojtaba Yousefi 
Published: September 2020

Abstract
Background: Sniffer dogs have been shown to detect certain chemical particles and to help diagnose many diseases and complications, such as colorectal cancer, melanoma, bladder cancer, and even dangerous states such as hypoglycemia in DM-1 patients. With the spread of COVID-19 throughout the world and the need to have a real-time screening of the population, especially in crowded places, this study aimed to investigate the applicability of these sniffer dogs.Methods: This study was done in two phases. In the first step, three dogs (including one German shepherd, one German black, one Labrador) were intensively trained by the classical conditioning method for seven weeks. Human specimens were obtained from the throat culture and pharyngeal secretions of both definitely positive and negative COVID-19 participants. During the first seven weeks, each dog underwent the conditioning process for averagely 1000 times. In the verification process, 80 pharyngeal secretion samples consisting of 26 positive samples of hospitalized patients and 54 negative samples of hospitalized patients for other medical reasons were provided to the training team in a single-blind manner. The verification test was done using three dogs (Lexi, Sami, and Kozhi). Another similar conditioning process was done using COVID-19 patients’ clothes and masks and the verification test was done using 50 positive and 70 negative samples by three other trained dogs (one Labrador, one Border gypsy, and one Golden retriever). Results: In the pharyngeal secretions verification test, the sniffer dogs showed 65% sensitivity and 89% specificity. In this process, they could identify 17 out of the 26 true positive samples and 48 out of the 54 true negative samples. According to the results, the positive predictive value and negative predictive value for this experiment were 74% and 84%, respectively. In the next verification test for the patients’ face masks and clothes, 43 out of the 50 positive samples were correctly identified by the dogs. Moreover, out of the 70 negative samples, 65 samples were correctly found to be negative by the canines. The sensitivity of this method was as high as 86% and its specificity was 92.9%. In addition, the positive and negative predictive values were 89.6% and 90.3%, respectively.Conclusion: Dogs are capable of being trained as the identifier of people with COVID-19 by detecting their odor and can be used as a reliable tool in limited screening.
Artemisinin attenuated oxidative stress and apoptosis by inhibiting autophagy in MPP+-treated SH-SY5Y cell
Authors: Yan Junqiang , Ma Hongxia , Lai Xiaoyi , Wu Jiannan , Liu Anran , Huang Jiarui , Sun Wenjie , Shen Mengmeng , Zhang Yude 
Published: September 2020

Abstract
Background
Parkinson's disease (PD) is the second most common neurodegenerative disease after Alzheimer's. The drugs currently used to treat PD cannot inhibit the development of PD, and long-term use produces severe drug resistance and adverse reaction. Artemisinin (ART) is an active ingredient of Artemisia annua and has a neuroprotective effect, but the mechanism is still unclear. This study was designed to investigate the neuroprotective effect of ART in MPP+-treated SH-SY5Y cells.
Results
There was no significant cytotoxicity when the ART concentration was under. 40 µM. The 20 µM ART for 24 h could increase the cell viability by reducing oxidative stress and cell apoptosis in MPP+-treated SH-SY5Y cell. In addition, immunoblot and immunofluorescence results showed that MPP+ treatment increased the expression of Beclin1, LC3II/LC3I and decreased the expression of P62, while ART can reverse the changes caused by MPP+.
Discussion
More and more researches reported that ART and its derivates have neuroprotective effects through anti-oxidant and anti-apoptosis. we found that pre-treated cells with 20 µM ART for 4 h could significantly increase the viability in Parkinson's disease cell model. The oxidative stress and apoptosis were the main reason for the degeneration of dopaminergic neurons, while artemisinin can attenuate oxidative stress and apoptosis in MPP+-lesioned dopaminergic neurons. The levels of autophagy proteins LC3II/I, Beclin1 and P62 also showed that MPP + increased the autophagy level, and pre-treatment with ART decreased the autophagy level, which may be the pathological mechanism for artemisinin to reduce oxidative stress damage and apoptosis.
Conclusions
These results indicate that ART exerts a positive effect on MPP+-treated SH-SY5Y cells in terms of anti-oxidative stress and anti-apoptosis. These effects may be related to autophagy. These findings contribute to a better understanding of the critical role of ART in PD treatment.