bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combining Rotation and V-Y Design for Lower Forehead and Temporal Region Reconstruction With Myocutaneous Frontal Flap
Authors: Righi Bernardo , Robotti Enrico , Carminati Marcello 
Published: February 2020

Background
Because of direct sun exposure, lower forehead and temporal area are anatomical regions with frequently observed skin tumors. The resulting defects after oncological ablations are usually treated, especially in older patients, by split thickness skin grafting or A-to-T and O-to-T flaps if facing small defects. On specific sites that expose bone or periosteum as well as when a skin graft is best avoided for cosmetic reasons, alternative reconstructive procedures should be considered. An excellent option is the use of myocutaneous frontal flap with rotation and V-Y design, which provide a reliable coverage of defects especially of the temporal area, dispensing appropriate like-tissue by its wide arc of rotation.

Methods
Between 2010 and 2019, 27 patients underwent myocutaneous frontal flap with rotation and V-Y design reconstruction of medium-to-large-sized soft-tissue defects of the lower forehead and temporal area after tumor excision. The malignant skin lesions involved were basal cell carcinoma (n = 17), squamous cell carcinoma (n = 9), and melanoma (n = 1). Twenty one patients were male, and 6 were female with an average age of 64 (54–86) years.

Results
All defects were successfully resurfaced with no local recurrences.

Conclusions
The authors present a modification to myocutaneous frontal flap harvesting, with emphasis on its extensive arc of rotation with a V-Y design to minimize donor-site morbidity. The advantages of myocutaneous frontal flap with rotation and V-Y design include a good contour with excellent color, texture, and thickness match and good to excellent aesthetic results.
Human transposon insertion profiling by sequencing (TIPseq) to map LINE-1 insertions in single cells
Authors: McKerrow Wilson , Tang Zuojian , Steranka Jared P. , Payer Lindsay M. , Boeke Jef D. , Keefe David , Fenyö David , Burns Kathleen H. , Liu Chunhong 
Published: February 2020

Long interspersed element-1 (LINE-1, L1) sequences, which comprise about 17% of human genome, are the product of one of the most active types of mobile DNAs in modern humans. LINE-1 insertion alleles can cause inherited and de novo genetic diseases, and LINE-1-encoded proteins are highly expressed in some cancers. Genome-wide LINE-1 mapping in single cells could be useful for defining somatic and germline retrotransposition rates, and for enabling studies to characterize tumour heterogeneity, relate insertions to transcriptional and epigenetic effects at the cellular level, or describe cellular phylogenies in development. Our laboratories have reported a genome-wide LINE-1 insertion site mapping method for bulk DNA, named transposon insertion profiling by sequencing (TIPseq). There have been significant barriers applying LINE-1 mapping to single cells, owing to the chimeric artefacts and features of repetitive sequences. Here, we optimize a modified TIPseq protocol and show its utility for LINE-1 mapping in single lymphoblastoid cells. Results from single-cell TIPseq experiments compare well to known LINE-1 insertions found by whole-genome sequencing and TIPseq on bulk DNA. Among the several approaches we tested, whole-genome amplification by multiple displacement amplification followed by restriction enzyme digestion, vectorette ligation and LINE-1-targeted PCR had the best assay performance.
This article is part of a discussion meeting issue ‘Crossroads between transposons and gene regulation’.
RNP-MaP: In-cell analysis of protein interaction networks defines functional hubs in RNA
Authors: Weidmann Chase A. , Mustoe Anthony M. , Jariwala Parth B. , Calabrese J. Mauro , Weeks Kevin M. 
Published: February 2020

ABSTRACTRNAs interact with networks of proteins to form complexes (RNPs) that govern many biological processes, but these networks are currently impossible to examine in a comprehensive way. We developed a live-cell chemical probing strategy for mapping protein interaction networks in any RNA with single-nucleotide resolution. This RNP-MaP strategy (RNPnetwork analysis bymutationalprofiling) simultaneously detects binding by and cooperative interactions involving multiple proteins with single RNA molecules. RNP-MaP revealed that two structurally related, but sequence-divergent noncoding RNAs, RNase P and RMRP, share nearly identical RNP networks and, further, that protein interaction network hubs identify function-critical sites in these RNAs. RNP-MaP identified numerous protein interaction networks within the XIST long noncoding RNA that are conserved between mouse and human RNAs and distinguished communities of proteins that network together on XIST. RNP-MaP data show that the Xist E region is densely networked by protein interactions and that PTBP1, MATR3, and TIA1 proteins each interface with the XIST E region via two distinct interaction modes; and we find that the XIST E region is sufficient to mediate RNA foci formation in cells. RNP-MaP will enable discovery and mechanistic analysis of protein interaction networks across any RNA in cells.
RNase L amplifies Interferon signaling by inducing PKR-mediated antiviral stress granules
Authors: Manivannan Praveen , Siddiqui Mohammad Adnan , Malathi Krishnamurthy 
Published: February 2020

ABSTRACTVirus infection leads to activation of the interferon-induced endoribonuclease, RNase L, which results in degradation of viral and cellular RNAs. Both cellular and viral RNA cleavage products of RNase L bind pattern recognition receptors (PRR) like Retinoic acid-inducible I (Rig-I) and or melanoma differentiation-associated protein 5 (MDA5) to further amplify interferon (IFN) production and antiviral response. Although much is known about the mechanics of ligand binding and PRR activation, how the cells coordinate RNA sensing to signaling response and interferon production remains unclear. We show that RNA cleavage products of RNase L activity induce formation of antiviral stress granule (avSG) by regulating activation of double-stranded RNA (dsRNA)-dependent protein kinase R (PKR), and recruit antiviral proteins Rig-I, PKR, OAS and RNase L to avSG. Biochemical analysis of purified avSG showed interaction of key stress granule protein, G3BP1, with only PKR and Rig-I and not with OAS or RNase L. AvSG assembly during RNase L activation is required for IRF3-mediated IFN production and not IFN signaling or proinflammatory cytokine induction. Consequently, cells lacking avSG formation or RNase L signaling produced less IFN and showed higher susceptibility during Sendai virus infection demonstrating the importance of avSG in RNase L-mediated host defense. During viral infection, we propose a role for RNase L-cleaved RNAs in inducing avSG containing antiviral proteins to provide a platform for efficient interaction of RNA ligands with pattern recognition receptors to enhance IFN production to effectively mount antiviral response.IMPORTANCEDouble-stranded RNAs produced during viral infections serve as pathogen associated molecular patterns (PAMPs) and bind pattern recognition receptors to stimulate IFN production. RNase L is an IFN-regulated endoribonuclease that is activated in virus-infected cells and cleaves single-stranded viral and cellular RNAs. The RNase L-cleaved dsRNAs signal to Rig-like helicases to amplify IFN production. This study identifies a novel role of antiviral stress granules induced by RNase L as an antiviral signaling hub to coordinate the RNA ligands with cognate receptors to mount effective host response during viral infections.
Defining endemism levels for biodiversity conservation: tree species in the Atlantic Forest hotspot
Authors: Lima Renato A.F. , Souza Vinicius Castro , de Siqueira Marinez Ferreira , ter Steege Hans 
Published: February 2020

AbstractEndemic species are important for biodiversity conservation. Yet, quantifying endemism remains challenging because endemism concepts can be too strict (i.e., pure endemism) or too subjective (i.e., near endemism). We propose a data-driven approach to objectively estimate the proportion of records inside a given the target area (i.e., endemism level) that optimizes the separation of near-endemics from non-endemic species. We apply this approach to the Atlantic Forest tree flora using millions of herbarium records retrieved from multiple sources. We first report an updated checklist of 5044 species for the Atlantic Forest tree flora and then we compare how species-specific endemism levels obtained from herbarium data match species-specific endemism accepted by taxonomists. We show that an endemism level of 90% separates well pure and near-endemic from non-endemic species, which in the Atlantic Forest revealed an overall endemism ratio of 45% for its tree flora. We also found that the diversity of pure and near endemics and of endemics and overall species was congruent in space. Our results for the Atlantic Forest reinforce that pure and near endemic species can be combined to quantify regional endemism and therefore to set conservation priorities taking into account endemic species distribution. We provided general guidelines on how the proposed approach can be used to assess endemism levels of regional biotas in other parts of the world.
Transient analysis of impatient customers in an M/M/1 disasters queue in random environment
Authors: Ammar Sherif I. , Jiang Tao , Ye Qingqing 
Published: February 2020

Purpose
This paper aims to consider a single server queue with system disasters and impatience behavior are evident in our daily life. For this purpose, authors require to know the general behavior of these systems. Transient analysis shows for us how the system will operate up to some time instant t.

Design/methodology/approach
In this paper, authors consider a single server queue with system disaster and impatient behavior of customers in a multi-phase random environment, in which the system transits to a repair state after each system disaster. When the system is in a failure phase or going through a repair phase, the new arrivals would be impatient. In case the system is not repaired before the customer’s time expires, the customer would leave the queue and never return. Moreover, after repair, the system becomes ready for service in an operative phase with probability $q_{i} \ge 0.$. Using generating functions along with continued fractions and some properties of the confluent hypergeometric function, authors obtained on their own results.

Findings
Explicit expressions have been obtained for the time-dependent probabilities of the underlying queuing model. Also, time-dependent mean and variance of customers in the system are deduced.

Research limitations/implications
The system authors are dealing with is somewhat complicated, there are some performance measures that cannot be achieved, but some of them have been obtained, such as the expectation and variance of the number of customers in the system.

Practical implications
Based on the obtained results, some numerical examples are some numerical examples are presented to illustrate the effect of various parameters on the behavior of the proposed system.

Social implications
Authors’ studied transient analysis of a single server queue with system disaster and impatient customer system is suitable for behavior interpretation of many systems in our lives, such as telecommunication networks, inventory systems and impatient telephone switchboard customers, manufacturing system and service system.

Originality/value
To the best of the author’s/authors’ knowledge and according to the literature survey, in a multi-phase random environment, no previous published article is presented for transient analysis of a single server queue with system disaster and impatient customer behavior in a random environment.
A novel approach for nonlinear equations occurs in ion acoustic waves in plasma with Mittag-Leffler law
Authors: Veeresha P. , Prakasha D.G. , Singh Jagdev 
Published: February 2020

Purpose
The purpose of this paper is to find the solution for special cases of regular-long wave equations with fractional order using q-homotopy analysis transform method (q-HATM).

Design/methodology/approach
The proposed technique (q-HATM) is the graceful amalgamations of Laplace transform technique with q-homotopy analysis scheme and fractional derivative defined with Atangana-Baleanu (AB) operator.

Findings
The fixed point hypothesis considered to demonstrate the existence and uniqueness of the obtained solution for the proposed fractional-order model. To illustrate and validate the efficiency of the future technique, the authors analysed the projected nonlinear equations in terms of fractional order. Moreover, the physical behaviour of the obtained solution has been captured in terms of plots for diverse fractional order.

Originality/value
To illustrate and validate the efficiency of the future technique, we analysed the projected nonlinear equations in terms of fractional order. Moreover, the physical behaviour of the obtained solution has been captured in terms of plots for diverse fractional order. The obtained results elucidate that, the proposed algorithm is easy to implement, highly methodical, as well as accurate and very effective to analyse the behaviour of nonlinear differential equations of fractional order arisen in the connected areas of science and engineering.
The multi-level effects of developmental opportunities, pay equity, and paternalistic leadership on organizational commitment
Authors: Chai Dae Seok , Jeong Shinhee , Joo Baek-Kyoo 
Published: February 2020

Purpose
The purpose of this study is to examine the effects of developmental opportunities and perceived pay equity-and paternalistic leadership on affective organizational commitment and the moderating role of paternalistic leadership at the team level in a Korean context.

Design/methodology/approach
Hierarchical linear modeling with a two-level design was used to analyze data collected from 844 employees and 59 work teams.

Findings
The study identified that developmental opportunities and perceived pay equity were significantly associated with affective organizational commitment. However, paternalistic leadership was not significantly related to affective organizational commitment. The results also showed that the moderation effect of paternalistic leadership on the relationship between pay equity and organizational commitment was non-significant, and paternalistic leadership moderated the relationship between developmental opportunities and organizational commitment. In particular, the relationship of developmental opportunities with organizational commitment became weaker when the supervisor’s paternalistic leadership was stronger.

Research limitations/implications
The results of this study supported the applicability of organizational support theory and previous empirical studies supporting the relationships between human resource (HR) practices and commitment, particularly in the Korean cultural context. The results have several practical implications for employers, mangers and HR practitioners in an East Asian cultural context.

Originality/value
This study extends the body of knowledge in leadership research by investigating the influences of two key factors of HR practices and a Confucianism-based indigenous leadership theory on organizational commitment. More importantly, the results can guide future cross-national or cross-organizational studies exploring the relationships among leadership, organizational culture and organizational effectiveness. This study also offers clearer empirical evidence for why and how developmental opportunities and perceived pay equity need to be enhanced in an East Asian cultural context.
Training Ukrainian military in job creation and job taking
Authors: Kolvereid Lars , Iermolenko Olga 
Published: February 2020

PurposeThe purpose of this study is to examine the effectiveness of courses offered to Ukrainian military personnel and their families to facilitate transfer to the civilian society, and to investigate the extent to which transfer to the civilian sector is associated with increased quality of life.Design/methodology/approachData were collected from 1,077 former course participants by handing out a structured questionnaire at different locations in Ukraine.FindingsAmong the 40 courses arranged, 15 proved to be effective with regard to the odds of obtaining a civilian job, and 10 with regard to the odds of becoming a business owner. Business owners and civilian employees scored higher than military employees with regard to different indicators of quality of life. Individuals who are unemployed or not members of the workforce score lower on quality of life than any other group.Originality/valueThis study evaluates the courses offered in a large educational program where the Norwegian and Ukrainian government cooperate to facilitate transfer of military veterans in Ukraine to the civilian sector.
Do firms harvest from political connections during general elections? Case of Pakistan
Authors: Ashraf Ayesha , Hassan M. Kabir , Abbas Khurram , Zaman Qamar Uz 
Published: February 2020

Purpose
This paper aims to examine the impact of general elections on the stock returns of the politically connected group affiliated firms of Pakistan.

Design/methodology/approach
This study uses the market model to assess the impact of political connections (PCs) on abnormal stock returns, before and after election events. We have used share price data of non-financial firms of Pakistan for the years 2008-2013.

Findings
It has been found that behavior of cumulative average abnormal returns (CAAR) is significantly different for standalone and politically connected group affiliated firms. The results reveal that CAARs of politically connected group affiliated firms have experienced less deviation as compared to stand alone firms. Therefore, it is argued that politically connected group firms may reduce the impact of political uncertainty on stock returns in comparison to stand alone firms.

Practical implications
This study is helpful for policy regulators of Pakistan to devise appropriate policies to maintain a level playing field for politically connected and standalone firms.

Originality/value
This study provides a new dimension to understand the role and association of PCs and general elections with stock markets returns.
Leadership by fragmented destruction after a merger: an example from a facility of acute psychiatry
Authors: Berg John E. , Grimeland Jorid 
Published: February 2020

Hospitals are labor intensive facilities based on highly skilled employees. A merger of hospitals is an effort to increase and rationalize this production. Decisions behind a merger are made at the top leadership level. How this might be done is demonstrated by examples from a 36 bed acute psychiatric facility. The aim of the study was to calculate the hidden costs of fragmented destruction of parts of a total hospital supply to patients after a merger. Fragmented destruction is the deliberate stopping of activities deemed not part of the core activities of the hospital without due consideration of the impact on core activities. The proposed changes to operational expenses at a single acute psychiatric hospital were materials for the study. The changes included activities as a reduction in local laboratory service, cleaning services, closure of physiotherapy unit, closing of cultural activities and reduced productivity. The selected activities are calculated as giving an imputed gain of € 630,000 as indicated by the leadership. The not calculated costs of reducing or removing the selected activities are estimated at € 1,955,640. The cost of staff disappointment after a merger is difficult to assess, but is probably higher than assumed in the present calculations.
Imputed cost containment is not attained. The calculations indicate that implemented changes may increase cost, contrary to the belief of the leadership at both the hospital level and further up in the hospital trust.
Arguments in favor of a merger have to be scrutinized thoroughly for optimistic neglect of uncalculated costs of mergers. Future hospital mergers and selected fragmentation of productive tasks at ward or hospital levels should include calculations of unavoidable costs as shown in the present paper.
Deep brain stimulation for obsessive compulsive disorder: A review of results by anatomical target
Authors: Borders Candace , Hsu Frank , Sweidan Alexander J. , Matei Emily S. , Bota Robert G. 
Published: February 2020

Studies suggest deep brain stimulation (DBS) as a treatment modality for the refractory obsessive-compulsive disorder (OCD). It is unclear where to place the DBS. Various sites are proposed for placement with the ventral capsule/ventral striatum (VC/VS) among the most studied. Herein, we aim to summarize both quantitative Yale-Brown Obsessive-Compulsive Scale (YBOCS) data and qualitative descriptions of the participants' symptoms when given. A literature search conducted via PubMed yielded 32 articles. We sought to apply a standard based on the utilization of YBOCS. This yielded 153 distinct patients. The outcome measure we focused on in this review is the latest YBOCS score reported for each patient/cohort in comparison to the location of the DBS. A total of 32 articles were found in the search results. In total, 153 distinct patients' results were reported in these studies. Across this collection of papers, a total of 9 anatomic structures were targeted. The majority of studies showed a better response at the last time point as compared to the first time point. Most patients had DBS at nucleus accumbens followed by VC/VS and the least patients had DBS at the bilateral superolateral branch of the median forebrain bundle and the bilateral basolateral amygdala. The average YBOCS improvement did not seem to directly correlate with the percentile of patients responding to the intervention.
Well-controlled, randomized studies with larger sample sizes with close follow up are needed to provide a more accurate determination for placement of DBS for OCD.