bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of activated carbon block point‐of‐use filters on chloraminated water quality
Authors: Alfredo Katherine , Lin Jie , Islam Anjuman , Wang Zhi‐Wu 
Published: June 2020

AbstractWashington, District of Columbia, installed activated carbon‐based lead mitigation filters on all water fountains/sinks in break rooms, classrooms, and health suites located in public elementary schools. To investigate the impact of these point‐of‐use filters, 12 water fountains/taps with various filter ages, with and without sediment prefilters, spread across four schools were monitored weekly from July to December 2017, along with two unfiltered sources per school (n = 8). Within the first 100 days postinstallation, filtrate from filters in schools with high influent monochloramine and no prefilter temporarily increased nitrite concentrations, with concentrations of some filters exceeding 0.4 mg/L N. Subsequent decreases in filtrate nitrite for these filters coincided with increases in live cell counts. Microbial community “fingerprints” were determined with flow cytometry. Filtered and unfiltered water fingerprints differed, indicating selective microbial growth on, and release from, the filters into the filtrate.
Relative energy response of indigenously developed optically stimulated luminescence dosimeters Al2O3:C, LiMgPO4:B and LiCaAlF6:Eu,Y in therapeutic photon and electron beams
Authors: Kumar Pradip , Dhabekar Bhushan , Sharma Sunil Dutt , Mishra Devesh Ramdhar , Rawat Narender Singh , Kadam Sonal , Chaudhari Suresh , Chandola Rakesh Mohan , Agrawal Sadhana 
Published: June 2020

AbstractThe relative energy responses of three indigenously developed optically stimulated luminescence (OSL) phosphors in the disc form were studied in therapeutic photon and electron beams. Calibration in terms of absorbed dose was carried out in the dose range 5–500 cGy in 60Co gamma rays, high energy X‐rays, and electron beams used in radiotherapy. The combined standard uncertainty in the estimation of absorbed dose using these OSL discs (OSLDs) was 3.3%. Dose–response curves of these OSLDs in 60Co gamma rays, 6 and 10 MV (flat and unflat), 15 MV and 6 and 15 MeV electron beams were found to be linear. Furthermore, these OSLDs exhibited a relative energy‐dependent response for both photon and electron beams. The relative energy response correction factor for photon and electron beams were in the range 1.01–1.05 and 1.03–1.06, respectively.
Fe(III) bipyridyl or phenanthroline complexes with oxidase‐like activity for sensitive colorimetric detection of glutathione
Authors: Zhang Liu , Hou Yue , Guo Xiaoyan , Liu Wei , Lv Congcong , Zhang Chunyang , Jin Yan , Li Baoxin 
Published: June 2020

AbstractIn this study, three types of Fe(III) bipyridyl or phenanthroline (Fe(III)‐L3) complex could directly catalyze 3,3′,5,5′‐tetramethylbenzidine (TMB) to induce blue chromogenic changes without H2O2. Fe(III)‐L3 complex could induce a colour change in TMB directly after a short incubation time. Due to the high oxidase‐like activity of the Fe(III)‐L3 complexes, superoxide anion radicals (O2•–) were formed in solution. Intermediates radical involving oxo‐iron species were then produced that oxidized TMB to its oxidation products (oxTMB), which had an absorbance maximum at 652 nm. Glutathione (GSH) could inhibit the oxidation reaction of the Fe(III)‐L3 complex–TMB system, a rapidly colorimetric method was established for the specific detection of GSH that had a detection limit of 0.1 μM. Furthermore, Fe(III)‐L3 complexes could catalyze TMB to oxTMB directly without H2O2. This fast and simple colorimetric method may open a new avenue for application in the point‐of‐care diagnosis field using the TMB chromogenic system.
Nachrichten des Verbandes Biologie, Biowissenschaften & Biomedizin in Deutschland
Authors:   Arbeitskreis Gesundheit & Biologie
Published: June 2020

Wissenschaft & GesellschaftStellungnahme zur COVID‐19‐PandemieNagoya‐ProtokollAusbildung & KarriereArs legendi‐Fakultätenpreis Biowissenschaften 2020AK Gesundheit & Biologie im VBIOLandesverbändeBerlin‐Brandenburg & HessenVeranstaltungenSummer School
Sustainable development and circular economy: The role of institutional promotion on circular consumption and market competitiveness from a multistakeholder engagement approach
Authors: Alonso‐Almeida María del Mar , Rodríguez‐Antón José Miguel , Bagur‐Femenías Llorenç , Perramon Jordi 
Published: June 2020

AbstractThe transition from a linear economy to a circular economy (CE) is a real challenge to achieve long‐term sustainability. To push CE in the market, institutional promotion could become a key driver to positively impact both circular consumption and the competitiveness of the market. This paper analyzes the influence that soft and hard initiatives have on circular consumption and market competitiveness. Based on a survey of 1,281 respondents from different types of stakeholders, structural equations modeling statistical analysis was run. Results show that soft initiatives support the achievement of both objectives, whereas hard ones only influence greater circular consumption. However, the perception of the different stakeholders considered is very heterogeneous. It is indicative that not all institutional promotion initiatives are effective. Thus, institutions should guide, in an adequate and differentiated manner, their efforts to promote CE and sustainable development depending on the stakeholder they are targeting.
OSW‐1 inhibits tumor growth and metastasis by NFATc2 on triple‐negative breast cancer
Authors: Ding Xiaorong , Li Yumei , Li Jun , Yin Yongmei 
Published: June 2020

AbstractOSW‐1 is a natural compound extracted from the bulbs of Ornithogalum saundersiae in 1992. It has been shown strong antitumor activities in various cancer cells. However, the effects of OSW‐1 on tumor growth and metastasis in breast cancer are still poorly understood. In our research, we showed that OSW‐1 had a strong anticancer effect on breast cancer cells, but lower toxicity to normal cells. Accordingly, it also revealed significant inhibition of tumor growth by OSW‐1 in xenograft model. In addition, we performed Annexin V/PI‐labeled flow cytometric assay and TUNEL assay and showed that OSW‐1 inhibited tumor growth by inducing apoptosis. Furthermore, we carried out transwell assays and found that OSW‐1 significantly repressed the migratory and invasive capabilities of triple‐negative breast cancer (TNBC) cells via mediating epithelial‐mesenchymal transition. Besides, OSW‐1 also could inhibit metastasis in an orthotopic model and resulted in a longer survival compared with control group. Finally, we performed RNA‐sequencing and cellular functions to investigate the molecular mechanism of how OSW‐1 inhibits TNBC, and identified NFATc2 may as a pivotal factor for OSW‐1‐mediated effects on cell death, tumor growth, invasion, and migration.
Silence of TPPP3 suppresses cell proliferation, invasion and migration via inactivating NF‐κB/COX2 signal pathway in breast cancer cell
Authors: Ren Qianfeng , Hou Yugui , Li Xiaoying , Fan Xiaoe 
Published: June 2020

Malignant phenotypes are leading causes of death in patients with breast cancer (BC). Previously, it has been proved that tubulin polymerization promoting protein 3 (TPPP3) participates in cell progressions in several human cancers. Little is known about the functions of TPPP3 in BC. Herein, we detected the expression of TPPP3 in 54 clinical BC tissues and two BC cell lines by immunohistochemistry and Western blot. CCK‐8, wound healing, colony formation and Transwell assays were used to assess cell proliferation, clone formation, invasion and migration of MCF‐7 and T47D cells after transfection with TPPP3 siRNA. Meanwhile, related‐proteins expression was detected using Western blot. TPPP3 was found to be highly expressed in the tissues from the patients with BC. Poor outcomes were associated with the high expression of TPPP3 in all patients with BC. When MCF‐7 and T47D cells receiving TPPP3 siRNA transfection, the capacities of proliferation, clone formation, invasion and migration were suppressed and the expression of MMP‐2/−9 and NF‐κB p65/COX2 was notably reduced. The dual‐luciferase reporter assay indicated that the promoter regions of NF‐κB p65 could combine to TPPP3. Overall, the present study demonstrated that TPPP3 played a significant role in BC, and its inhibition lead to the suppression of NF‐κB/COX‐2 signalling pathway along with the reduction of malignant phenotypes.Significance of this studyPreviously, it has been proved that tubulin polymerization promoting protein 3 (TPPP3) participates in cell progression in several human cancers. Little is known about the function of TPPP3 in BC. Our study was the first direct evidence to support the role of TPPP3 in tumorigenesis and metastasis of BC. Although the underlying mechanism has not been fully delineated, these findings suggested that TPPP3 was an important factor in the tumour progression and metastasis of BC cells and provided a molecular basis for potential therapeutic implications in the treatment of patients with BC.
Changes of Endocan and its effect on hepatic stem cells during the rapid proliferation process of residual liver after ALPPS procedure
Authors: Zhao Liang , Dai Chaoliu , Gong Qi 
Published: June 2020

To establish a stable animal model of associating liver partition and portal vein ligation for staged hepatectomy (ALPPS), as well as to explore whether Endocan and HGF/c‐Met signalling pathway participate in the regeneration of residual liver through hepatic stem cells after ALPPS procedure. C57BL/6J male mice weighing 18‐22 g were used in this study. The liver regeneration index was expressed as the ratio of Future Liver Remnant (FLR)/Body weight (BW) × 100%. Expression of hepatic stem cell marker CK19 was assessed by immunohistochemical method. Serum levels of vascular endothelial growth factor (VEGF) and Endocan were detected by ELISA. VEGF, Endocan and c‐Met contents in residual liver were observed by Western blot analysis. The expression levels of Endocan and HGF/c‐Met pathway‐related genes were evaluated by qRT‐PCR. Compared with the portal vein ligation (PVL) group and sham group, the ALPPS group had more CK19 positive cells and a higher liver regeneration index (P < .05). The serum levels of VEGF in the ALPPS group were increased significantly (P < .05) from the first day and decreased from the second day after surgery, and maintained consistently higher than that of the sham group (P < .05). Western blot showed that the expressions of VEGF and Endocan in ALPPS group were significantly higher than those in both sham and PVL groups at different time points. The Endocan plays a role in the rapid regeneration of residual liver after ALPPS, likely through the interaction with the HGF/c‐Met pathway to affect the hepatic stem cell population.Significance of the studyOur animal study provides valuable insights on the effect of Endocan in the process of rapid regeneration of residual liver after ALPPS, which would lead to the possible expansion of clinical research on ALPPS surgery and further studies on its molecular underpinning during liver regeneration.
Evaluation of multiple linear regression applied to impedimetric sensing
Authors: Rodrigues Dayvison Ribeiro , Fragoso Wallace Duarte , Lemos Sherlan Guimarães 
Published: June 2020

AbstractIn this work, the application of multiple linear regression (MLR) on the development of electroanalytical methods based on electrochemical impedance spectroscopy (EIS) data was investigated. This approach was proposed considering that more than one element of the equivalent electrical circuit fitted to impedance spectra could keep linear relationship with the analyte concentration and be used in a multivariate calibration model, rather than using only one element of the circuit in a univariate regression, the benchmark procedure in the context of impedimetric (bio)sensing. First, MLR was evaluated in the individual determination of the redox probe ferrocyanide in aqueous solutions, followed by the determination of catechol and hydroquinone in tap water samples. MLR produced better predictions than univariate regression when more complex electrochemical systems (catechol and hydroquinone) and more complex samples (tap water) were analyzed. The determinations of hydroquinone and catechol in the direct analysis of spiked tap water samples were successful, with apparent recoveries ranging from 94% to 136%.
Synthesis of Aluminophosphate Molecular Sieves in Alkaline Media
Authors: Sheng Na , Ma Ye , Zhu Qianwen , Hong Xin , Zhang Juan , Xu Jun , Deng Feng , Sun Junliang , Feng Zhaochi , Wang Liang , Meng Xiangju , Xiao Feng‐Shou 
Published: June 2020

AbstractUnlike conventional aluminosilicate zeolites synthesized in alkaline media, aluminophosphate molecular sieves (AlPOs) have always been prepared under acidic conditions in the past three decades; this has been regarded as one of essential factors for synthesis, except for the case of silica‐substituted analogues (SAPOs). For the first time, we demonstrate herein a simple and generalized route for synthesizing various types of aluminophosphate molecular sieves in alkaline media. A series of aluminophosphate sieves and their analogues have been prepared with different quaternary ammonium cations as structure‐directing agents in this manner. The above successes have extended the systematic media from acidic or neutral to alkaline for the preparation of a series of aluminophosphate molecular sieves, which possibly open an alternative route for the synthesis of aluminophosphate molecular sieves.
Photocatalytic Conversion of Benzyl Alcohols/Methyl Arenes to Aryl Nitriles via H‐Abstraction by Azide Radical
Authors: Shee Maniklal , Shah Sk. Sheriff , Singh N. D. Pradeep 
Published: June 2020

AbstractThis report presents the visible‐light‐assisted synthesis of aryl nitriles from easily accessible alcohols or methyl arenes in the presence of O2. Organic photoredox catalyst, 4CzIPN (1,2,3,5‐tetrakis(carbazol‐9‐yl)‐4,6‐dicyanobenzene), induces single electron transfer (SET) from azide N3− and generates azide radical N3⋅.The photogenerated N3⋅ abstracts H atom from α‐C−H bond of benzylic system, which provides aldehyde and hydrazoic acid (HN3) in situ. This reaction subsequently forms azido alcohol intermediate that transforms into nitrile with the assistance of triflic acid (Brønsted acid). A range of alcohols and methyl arenes successfully underwent cyanation at room temperature with good to excellent yields and showed good functional group tolerance.
Degeneracy of the Antithrombin Binding Sequence in Heparin: 2‐O‐Sulfated Iduronic Acid Can Replace the Critical Glucuronic Acid
Authors: Elli Stefano , Stancanelli Eduardo , Wang Zhangjie , Petitou Maurice , Liu Jian , Guerrini Marco 
Published: June 2020

AbstractHeparin binds to and activates antithrombin (AT) through a specific pentasaccharide sequence, in which a trisaccharide subsite, containing glucuronic acid (GlcA), has been considered as the initiator in the recognition of the polysaccharide by the protein. Recently it was suggested that sulfated iduronic acid (IdoA2S) could replace this “canonical” GlcA. Indeed, a heparin octasaccharidic sequence obtained by chemoenzymatic synthesis, in which GlcA is replaced with IdoA2S, has been found to similarly bind to and activate antithrombin. By using saturation‐transfer‐difference (STD) NMR, NOEs, transferred NOEs (tr‐NOEs) NMR and molecular dynamics, we show that, upon binding to AT, this IdoA2S unit develops comparable interactions with AT as GlcA. Interestingly, two IdoA2S units, both present in a 1C4‐2S0 equilibrium in the unbound saccharide, shift to full 2S0 and full 1C4 upon binding to antithrombin, providing the best illustration of the critical role of iduronic acid conformational flexibility in biological systems.
Direct Synthesis of Peptide‐Containing Silicones: A New Way to Bioactive Materials
Authors: Martin Julie , Wehbi Mohammad , Echalier Cécile , Hunger Sylvie , Bethry Audrey , Garric Xavier , Pinese Coline , Martinez Jean , Vezenkov Lubomir , Subra Gilles , Mehdi Ahmad 
Published: June 2020

AbstractA simple and efficient way to synthesize peptide‐containing silicone materials is described. Silicone oils containing a chosen ratio of bioactive peptide sequences were prepared by acid‐catalyzed copolymerization of dichlorodimethylsilane, hybrid dichloromethyl peptidosilane, and Si(vinyl)‐ or SiH‐functionalized monomers. Functionalized silicone oils were first obtained and then, after hydrosilylation cross‐linking, bioactive polydimethylsiloxane (PDMS)‐based materials were straightforwardly obtained. The introduction of an antibacterial peptide yielded PDMS materials showing activity against Staphylococcus aureus. PDMS containing RGD ligands showed improved cell‐adhesion properties. This generic method was fully compatible with the stability of peptides and thus opened the way to the synthesis of a wide range of biologically active silicones.
Synthesis of Helically π‐Extended N‐Confused Porphyrin Dimer via meso‐Bipyrrole‐Bridge with Near‐Infrared‐II Absorption Capability
Authors: Yamasumi Kazuhisa , Notsuka Yusuke , Yamaoka Yoshihisa , Mori Shigeki , Ishida Masatoshi , Furuta Hiroyuki 
Published: June 2020

AbstractQuinoidal dimeric porphyrin dye synthesis exhibiting second near‐infrared (NIR‐II) absorbability is described herein. A precisely designed meso‐pyrrolyl‐substituted N‐confused porphyrin possesses a distinct metal coordination site at the periphery. Nickel metalation of this compound led to the oxidative C−H coupling between adjacent α‐pyrrole rings, affording two dimeric complexes, which exhibited intense NIR‐II absorptions ranging from 1000 to 1400 nm. As was evidenced by decreased aromaticity, the quinoidal resonant structures contributed to the emergence of photoacoustic spectral capabilities in the NIR‐II window. Thus, the potential of these compounds as prototypical contrast agents in various bioimaging applications has been demonstrated.
The First OCCCO Pentadentate Chelates: Osmium Mediated Stepwise Oxidations of Terminal Alkynes by Pyridine N‐Oxide
Authors: Lin Jianfeng , Xu Qiannan , Lin Xinlei , Hua Yuhui , Chen Dafa , Ruan Yonghong , Zhang Hong , Xia Haiping 
Published: June 2020

Summary of main observation and conclusionOxygen atom transfer reactions between alkynes and heteroarene N‐oxides are currently mediated by transition metal, such as gold, ruthenium or rhodium, but they have never been catalyzed by osmium. Herein, we report stepwise oxidations of terminal alkynes mediated by an osmium carbolong complex, using pyridine N‐oxide as the oxidant, yielding novel aldehyde‐ or carboxylic acid‐coordinated osmapentalyne derivatives selectively. The mechanism was illustrated by density functional theory calculations, providing a new route for oxygen atom transfer between heteroarene N‐oxides and alkynes.
Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma
Authors: Pasquettaz Roxane , Kolotuev Irina , Rohrbach Antoine , Gouelle Cathy , Pellerin Luc , Langlet Fanny 
Published: June 2020

AbstractTanycytes are highly specialized ependymal cells that line the bottom and the lateral walls of the third ventricle. In contact with the cerebrospinal fluid through their cell bodies, they send processes into the arcuate nucleus, the ventromedial nucleus, and the dorsomedial nucleus of the hypothalamus. In the present work, we combined transgenic and immunohistochemical approaches to investigate the neuroanatomical associations between tanycytes and neural cells present in the hypothalamic parenchyma, in particular in the arcuate nucleus. The specific expression of tdTomato in tanycytes first allowed the observation of peculiar subcellular protrusions along tanycyte processes and at their endfeet such as spines, swelling, en passant boutons, boutons, or claws. Interestingly, these protrusions contact different neural cells in the brain parenchyma including blood vessels and neurons, and in particular NPY and POMC neurons in the arcuate nucleus. Using both fluorescent and electron microscopy, we finally observed that these tanycyte protrusions contain ribosomes, mitochondria, diverse vesicles, and transporters, suggesting dense tanycyte/neuron and tanycyte/blood vessel communications. Altogether, our results lay the neuroanatomical basis for tanycyte/neural cell interactions, which will be useful to further understand cell‐to‐cell communications involved in the regulation of neuroendocrine functions.
Persistent spectral graph
Authors: Wang Rui , Nguyen Duc Duy , Wei Guo‐Wei 
Published: June 2020

AbstractPersistent homology is constrained to purely topological persistence, while multiscale graphs account only for geometric information. This work introduces persistent spectral theory to create a unified low‐dimensional multiscale paradigm for revealing topological persistence and extracting geometric shapes from high‐dimensional datasets. For a point‐cloud dataset, a filtration procedure is used to generate a sequence of chain complexes and associated families of simplicial complexes and chains, from which we construct persistent combinatorial Laplacian matrices. We show that a full set of topological persistence can be completely recovered from the harmonic persistent spectra, that is, the spectra that have zero eigenvalues, of the persistent combinatorial Laplacian matrices. However, non‐harmonic spectra of the Laplacian matrices induced by the filtration offer another powerful tool for data analysis, modeling, and prediction. In this work, fullerene stability is predicted by using both harmonic spectra and non‐harmonic persistent spectra, while the latter spectra are successfully devised to analyze the structure of fullerenes and model protein flexibility, which cannot be straightforwardly extracted from the current persistent homology. The proposed method is found to provide excellent predictions of the protein B‐factors for which current popular biophysical models break down.