bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surveillance of the efficacy of artemisinin-piperaquine for the treatment of uncomplicated Plasmodium falciparum among children under five years in Est Mono district, Togo, 2017
Authors: Wang Qi , Lu Chenguang , Zhang Zhenyan , Yu Weisheng , Li Guoming , Pan Ziyi , Zhang Hongying , Wu Wanting , Yuan Yueming , Guo Jiawen , Liang Yuan , Huang Xinan , Guo Wenfeng , Li Changqing , Chen Meilan , Xu Qin , Song Jianping , Deng Changsheng 
Published: January 2020

Abstract
Background Malaria is a major public health problem in Togo. Guangzhou University of Chinese medicine of China, and the Ministry of Health and Social Security of Togo launched a nationwide artemisinin compound Mass Drug Administration Project in East Mono with a population of 150,000. Before launching the project, the sensitivity test of artemisinin piperaquine tablet was conducted in Elawagnon general clinic. On this background, we evaluated the efficacy and safety of artemisinin piperaquine in the treatment of uncomplicated falciparum malaria in children under five years of age. Methods In this study, children aged 6-59 months without complications of falciparum malaria were observed, and the selected cases were treated with artemisinin piperaquine. The patients were followed up for 28 days to observe the fever clearance time, parasitemia, gametophyte, cure rate, haemoglobin and msp-2 gene polymorphism. The primary end point was the 28-day cure rate, and PCR corrected reinfection and recrudescence. This research was conducted according to standardized WHO protocol for the assessment of the efficacy of anti-malarial treatment. Results A total of 91 children participated in the study. The adequate clinical and parasitological response (ACPR) before PCR-corrected were 66 (72.52%) and 90 (98.90%) after PCR-corrected. The patient was well tolerated to artemisinin piperaquine and no serious adverse reactions were observed. The average hemoglobin level increased by 0.05g/dl per day (p< 0.0001). The gametophyte doesn’t declined at the beginning of treatment, however, 14 days later, it dropped(D21:p<0. 05; D28: p< 0. 01). In the msp-2 gene polymorphism study of 24 children with positive parasite after treatment, 1 case of msp-2 with 3D7 haplotype and FC27 haplotype was reported, indicating that it’s recrudescence, with a frequency of 4.2% (1/24); The others maybe reinfection, with a frequency of 95.8% (23/24). Conclusion Artemisinin piperaquine was effective in treating uncomplicated falciparum malaria in children under 5 years of age in Togo and well tolerated. Plasmodium falciparum in Togo remains sensitive to artemisinin piperaquine, which could be used as a trial drug in the region. Trial Registration Trial registration: ECGPHCM No. B2017-054-01; MHSST AVIS N° 0001/2016/CBRS du 07 janvier 2016. Registered 17 March 2014, http://www.chinadrugtrials.org.cn/eap/main
Everywhere is Nowhere: A Systematic Review of Menstrual Health and Hygiene Management (MHHM) as a Human Right
Authors: Sood Suruchi , Stevens Sarah , Okumura Maho , Ramaiya Astha , Hauer Michael 
Published: January 2020

Abstract
Background: In many countries, stigma surrounds menstruation, which impacts several Sustainable Development Goals, including good health, quality education, gender equality, and water and sanitation. Despite its relationship with several development issues, menstrual health and hygiene management among adolescents has until recently been ignored by practitioners and researchers. This paper is a systematic review of existing literature and argues that menstrual health and hygiene management is a human rights issue, cross-cutting through development domains of health, education, nutrition, child protection, and water, sanitation, and hygiene. Methods: Four coders independently screened PubMed, Academic OneFile, and Google Scholar to obtain articles using iterations of the key terms: menstrual health; adolescents; health outcomes; education; nutrition; water, sanitation, and hygiene; and child protection. English language primary studies in peer-reviewed or grey literature articles published after 2000, that mentioned adolescents, health or hygiene, and menstruation or menstrual were included. A 9-item scale was used to rate selected full-text articles as strong, moderate, or weak. Synthesis of the results were narrative and examined the relationships between menstrual health and hygiene management and the cross-cutting domains.Results: In total, 28,745 articles were screened, with 84 articles included in the full-text review and quality assessment. Nine articles were coded as “strong” (10.8%), 46 (55.4%) as “moderate,” and 28 (33.7%) as “weak.” More than 60% of the articles examined the relationship between menstrual health and hygiene management and health (37.3%) or water, sanitation, and hygiene (25.3%). Only 11 manuscripts examined menstrual health and hygiene management as a cross-cutting issue impacting more than one development domain.Conclusions: Access to adequate facilities and menstrual health and hygiene management resources are the most common relationships documented within literature. However, there is little focus on menstrual health and hygiene management in the context of other development domains, highlighting the fact that poor menstrual health and hygiene management has not been studied as a human rights issue, negatively impacting millions of girls. Using a cross-cutting, human rights framework to address inadequate menstrual health and hygiene management is fundamental to promoting menstrual health and hygiene management with dignity among girls and women across the globe.
PGRMC1 effects on metabolism, genomic mutation and CpG methylation imply crucial roles in animal biology and disease
Authors: Thejer Bashar M. , Adhikary Partho P. , Teakel Sarah L. , Fang Johnny , Weston Paul A. , Gurusinghe Saliya , Anwer Ayad G. , Gosnell Martin , Jazayeri Jalal A. , Ludescher Marina , Gray Lesley-Ann , Pawlak Michael , Wallace Robyn H. , Pant Sameer D. , Wong Marie , Fischer Tamas , J. Elizabeth , Fehm Tanja N. , Neubauer Hans , Goldys Ewa M. , Quinn Jane C. , Weston Leslie A. , CAHILL Michael 
Published: January 2020

Abstract
Progesterone receptor membrane component 1 (PGRMC1) is often elevated in cancers, and exists in alternative states of phosphorylation. A motif centered on PGRMC1 Y180 was evolutionarily acquired concurrently with the embryological gastrulation organizer that orchestrates vertebrate tissue differentiation. Here, we show that mutagenic manipulation of PGRMC1 phosphorylation alters cell metabolism, genomic stability, and CpG methylation. Each of several mutants elicited distinct patterns of genomic CpG methylation. Mutation of S57A/Y180/S181A led to increased net hypermethylation, reminiscent of embryonic stem cells. Pathways enrichment analysis suggested modulation of processes related to animal cell differentiation status and tissue identity, as well as cell cycle control and ATM/ATR DNA damage repair regulation. We detected different genomic mutation rates in culture. A companion manuscript shows that these cell states dramatically affect protein abundances, cell and mitochondrial morphology, and glycolytic metabolism. We propose that PGRMC1 phosphorylation status modulates cellular plasticity mechanisms relevant to early embryological tissue differentiation.
Basic Leucine zipper (bZIP) transcription factor genes and their response to drought stress in ginseng, Panax ginseng C.A. Meyer
Authors: Li Hongjie , Chen Jing , Zhao Qi , Han Yilai , Sun Chunyu , Wang Kangyu , Wang Yanfang , Zhao Mingzhu , Chen Ping , Lei Jun , Wang Yi , Zhang Meiping 
Published: January 2020

Abstract
Background: As a famous and important medicinal herb in the world, ginseng contains numerous bioactive components that are remarkable for mankind's health. The basic leucine zipper (bZIP) transcription factor genes play important roles in many biological processes and plant response to abiotic and biotic stresses. Nevertheless, these genes remain unknown in ginseng. Results: Here, we report 91 bZIP genes, designated as PgbZIP genes, identified from ginseng. These PgbZIP genes were alternatively spliced into 273 transcripts. Phylogenetic analysis grouped the PgbZIP genes into ten groups, including A, B, C, D, E, F, G, H, I and S. Gene Ontology (GO) categorized the PgbZIP genes into a number of functional categories, suggesting that they have substantially diversified in functionality, even though their putative proteins share a number of conserved motifs. These 273 PgbZIP transcripts expressed quite differentially across 14 different tissues, the roots of different aged, and the roots of different cultivars. However, the expression of these transcripts was coordinated as they formed a co-expression network. Furthermore, we studied their response to drought stress in ginseng using five representatives of the PgbZIP genes, including PgbZIP25 , PgbZIP38 , PgbZIP39 , PgbZIP53 and PgbZIP54 . The results showed that these PgbZIP genes all responded to drought stress in ginseng, but the magnitudes of their response to drought stress varied. Conclusions: These results provide knowledge and resources for deeper functional analysis of PgbZIP genes and molecular tools for enhanced drought tolerance breeding in ginseng.
Histological and sex-determining genes expression effects of 17α-methyltestosterone on mandarin fish Siniperca chuatsi gonad development
Authors: Liu Shuang , Xu Peng , Liu Xuange , Guo Dingli , Chen Xiaoli , Bi Sheng , Lai Han , Zhao Xiaopin , Su Yuqing , Yi Huadong , Li Guifeng 
Published: January 2020

Abstract
Background 17α-methyltestosterone (MT) as a synthetic androgen has been widely used in sex reversal of fish research, but there was no published report on sex reversal of mandarin fish by MT. Moreover, comparative transcriptome analysis of testis and ovarian tissue is still lacking in mandarin fish. We used histological analyses together with RNA sequencing to characterize mandarin fish gonadal transcriptomes and investigate the effects of MT on the sex ratio, survival rate, growth, gonadal differentiation.ResultsMandarin fish treated with dietary MT at 50 mg/kg and 100 mg/kg dosages were successfully induced to all-male stock (male rate 100%), compared with the control group (51.11%). The survival rate of fish in the MT treated and control groups were not significant different. MT were significantly inhibited the growth of the MT treatment group ( P < 0.05) at the 20 dph-120 dph, however, the weight and length in the MT treated and control groups were not significant different at the 180 dph and 240 dph. MT treatment promoted the development of testis, but inhibited the gonadosomatic index (GSI) and the levels of serum steroid hormone (T and E 2 ). This work screened out the genes related to the sex determination and differentiation of the fish by sequencing and analysis of the transcriptome of the ovary and testis. The masculinization of mandarin fish was also demonstrated by the expression patterns of sex-specific genes, dmrt1, sox9, foxl2 and cyp19a1a : the gonads of MT-treated fish exclusively expressed male-specific dmrt1 and sox9 with no expression of female-specific foxl2 and cyp19a1a .Conclusion This study suggests that 17α-methyltestosterone successfully induced all-male stock and we select the part of the genes ( dmrt1, sox9, foxl2 and cyp19a1a ) related to sex determination and differentiation.
Genome-wide association study and genomic selection for plant height, maturity, seed weight, and yield in soybean
Authors: Ravelombola Waltram , Qin Jun , Shi Ainong , Wang Fengmin , Feng Yan , Meng Yaning , Yang Chunyan , Zhang Mengchen 
Published: January 2020

Abstract
BackgroundSoybean [Glycine max (L.) Merr.] is a legume of great interest worldwide. Enhancing genetic gain for agronomic traits via molecular approaches has been long considered as the main task for soybean breeders and geneticists. The objectives of this study were to conduct a genome-wide association study (GWAS) for these traits and identify SNP markers associated with the four traits, and to assess genomic selection (GS) accuracy.Results A total of 250 soybean accessions were evaluated for maturity, plant height, seed weight, and yield over three years. This panel was genotyped with a total of 10,259 high quality SNPs postulated from genotyping by sequencing (GBS). Population structure was inferred using STRUCTURE 2.3.4, GWAS was performed using a Bayesian Information and Linkage Disequilibrium Iteratively Nested Keyway (BLINK) model, and GS was evaluated using a ridge regression best linear unbiased predictor (rrBLUP) model. The results revealed that: a total of 20, 31, 37, 31, and 23 SNPs were significantly associated with the average 3-year data for maturity, plant height, seed weight, and yield, respectively; some significant SNPs were mapped into previously described loci (E2, E4, and Dt1) affecting maturity and plant height in soybean and a new locus mapped on chromosome 20 was significantly associated with plant height; Glyma.10g228900, Glyma.19g200800, Glyma.09g196700, and Glyma.09g038300 were candidate genes found in the vicinity of the top or the second best SNP (if no annotated genes found close the top one) for maturity, plant height, seed weight, and yield, respectively; a 11.5-Mb region of chromosome 10 was associated with both seed weight and yield; and GS accuracy was trait-, year-, and population structure-dependent.Conclusions The SNP markers identified from this study for plant height, maturity, seed weight and yield can be used to improve the four agronomic traits in soybean through marker-assisted selection (MAS) and GS in breeding programs. After validation, the candidate genes can be transferred to new cultivars using the linked SNP markers through MAS. The high GS accuracy has confirmed that the four agronomic traits can be selected in molecular breeding through GS.
MicroRNA-mRNA regulatory network related to lipid metabolism in bovine mammary epithelial cells
Authors: Xia Lixin , Zhao Zhihui , Lu Chunyan , Jiang Ping , Yu Haibin , Li Xiaohui , Yu Xiang , Liu Juan , Fang Xibi , Yang Runjun 
Published: January 2020

Abstract

Milk fat percentage is an important factor of milk quality in dairy cattle. Functional microRNAs and genes can affect lipid synthesis and metabolism through differential expression in bovine mammary epithelial cells (BMECs). It is necessary to screening the crucial candidate gene and miRNA on milk fat percentage. In this study, we extract total RNA of BMECs isolated from Chinese Holstein cows with high and low milk fat percentages for the conjoint analysis of RNA-seq and Solexa sequencing data. 190 differentially expressed genes and 33 differentially expressed microRNAs (DERs) were enriched in 488 GO terms and 12 KEGG pathways significantly (p <0.05) based on the conjoint analysis. The detection of triglyceride production in BMECs showed that bta-miR-21-3p and bta-miR-148a promote triglyceride synthesis, whereas bta-miR-124a, bta-miR-877, bta-miR-2382-5p and bta-miR-2425-5p inhibit triglyceride synthesis. Meanwhile, the target relationships between PDE4D and bta-miR-148a, PEG10 and bta-miR-877, SOD3 and bta-miR-2382-5p, and ADAMTS1 and bta-miR-2425-5p were verified using luciferase reporter assays and quantitative RT-PCR. The conjoint analysis can more accurately screen candidate regulator related to milk fat percentage at the molecular level, which provided a scientific research method screening functional miRNA and gene for the breeding of new high-quality dairy cows.
Inanimate figure tracing and ex vivo pig stomach models to teach endoscope control and ESD: Description of models and preliminary results.
Authors: Kumara HMC Shantha , Shah Abhinit , Pettke Erica , Sutton Elie , Bellini Geoffrey , Sandhu Jaspreet , Winkler Carl , Yan Xiaohong , Cekic Vesna , HMC Shantha Kumara , Gandhi Nipa Dilip , Whelan Richard L 
Published: January 2020

Abstract
Background ESD is the gold standard treatment for large sessile adenomas yet US adoption rates are low. In ESD, tissue is cut only when the scope tip, with hot knife extended, is moving; this requires different skills than needed for snare polypectomy (scope tip held still). Scope tip control and ESD training methods are needed. A program including an inanimate figure tracing model (FTM; Model1), ex vivo pig stomach model (PSM; Model2) and bovine colon model was devised. This paper concerns preliminary results for models 1 and 2.Methods A straight hollow plastic tube with a window cutout 15cm from its end was used for both models (slotted sponge = “anus”). For the FTM a paper with a line figure is placed in the window. After endoscope insertion and positioning the figure is traced with a catheter "pen"; 8 tracings (4 figure types) and 1 pig ESD were done per session. In the PSM a piece of pig stomach and Bovie pad was placed over the window. After “lifting” the mucosa ESD resection of a "polyp" was done. Time/accuracy were tracked for both models as were bowel wall injuries in the PSM.Results A total of 30 FTM sessions and PSM cases were done (2 trainees). Completion times and accuracy improved over 8 FTM sessions for less experienced trainee A but not for trainee B. A total of 29/30 PSM cases were completed. The number of deep muscle injuries decreased with time, however, case length correlated with tissue quality and not number of cases. Lifting and tissue conductivity problems were noted in poor quality tissue.Conclusions Despite model related issues, both models are feasible and allow trainees to learn scope tip control and ESD; clearly, more study is needed. A smaller diameter tube and use of fresh stomach are advised.
Removal of trihalomethanes from high organic matter water sources using aeration: a feasibility study
Authors: Mirzaei Saeideh , Gorczyca Beata 
Published: January 2020

Abstract
In this study, diffused aeration was applied to remove trihalomethane (THM) compounds from chlorinated, treated water containing high dissolved organic carbon (DOC) of 6.8 ± 1.2 mg/L. Increasing air-to-water volumetric ratio (rA/W) from 16 to 39 enhanced total THM (TTHM) removal from 60 to 70% at 20 °C and from 30 to 50% at 4 °C. Although bromodichloromethane has lower Henry's law constant than chloroform (CF), it was removed by a higher degree than CF in some aeration trials. Albeit obtaining high removals in aeration, TTHM reformed, and their concentration surpassed the Canadian guideline of 100 ppb in about 24 hours at 20 °C and 40 hours at 10 °C in all attempted air-to-water ratios. The water age in the system investigated in this study varied from 48 hours in midpoint chlorine boosting stations to 336 hours in the nearest endpoint. This study showed that THM removal by aeration is not a viable solution to control the concentration of these disinfection by-products in high-DOC treated water and in distribution systems where water age exceeds 24 hours; unless, it is going to be installed at the distribution endpoints.
Nalidixic Acid Schiff Bases: Synthesis and Biological Evaluation
Authors: Husain Asif , Varshney Munendra M. , Parcha Versha , Ahmad Aftab , Khan Shah A. 
Published: January 2020

Background:
The prevalence of morbidity and mortality due to infections from parasitic
worms and protozoa is on rise especially in third world countries. The situation is further worsened
by drug resistant microbial pathogens.

Objectives:
The antimicrobial and anthelmintic activities associated with substituted furfuraldehyde
and 1,8-naphthyridine nucleus of nalidixic acid prompted us to synthesize some new quinolone
Schiff bases with an aim to obtain potent antibacterial and anthelmintic agents with improved safety
and efficacy.

Methods:
A new series of 1,8 naphthyridine based Schiff bases were designed and synthesized by
the reaction of Nalidixic acid methyl ester 1 with hydrazine hydrate in anhydrous condition which
yielded 1-ethyl-1,4-dihydro-7-methyl-4-oxo-1,8-naphthyridine-3-carbohydrazide 2. The compound
2 was further treated with several furfural aldehydes to furnish the desired Schiff bases (3a-k).
The in vitro antibacterial activity of Schiff bases was investigated against four Gram positive and
four Gram negative bacterial strains. The newly prepared Schiff bases were also tested for their
anthelmintic activity against Pheritima posthuma and Perionyx excavatus.

Results:
Chemical structures and identity of the prepared compounds were confirmed by their
spectral data. Overall, Schiff bases (3a-k) showed good antimicrobial activity and interestingly five
compounds exhibited more potent inhibitory effect than the standard drug Ampicillin against
S. aureus, B. cereus, E. faecalis, S. epidermidis, E. coli, S. typhi and S. dysenteriae. Schiff bases
also exhibited significant anthelmintic activity as indicated by their mean paralyses time (min) of
7.07-16.49, and 11.23- 20.46 min against Perionyx excavatus and Pheritima posthuma in comparison
to the 8.23 and 12.58 min shown by standard drug- Albendazole.

Conclusion:
It could be proposed that substitution of aromatic ring at C-5 of furfuryl heterocyclic
ring in the Schiff bases produce compounds with promising antibacterial and anthelmintic actions.
PERUBAHAN TARI GANDAI PADA MASYARAKAT MUKOMUKO PROVINSI BENGKULU
Authors: Elyadi Marfinetri , Ningsih Lora Gustia 
Published: January 2020

AbstrakTari gandai merupakan sebuah tari tradisi yang ada di Mukomuko provinsi Bengkulu. Tari ini berasal dari cerita rakyat Malin Deman dan Puti Bungsu, Malin Deman merupakan manusia bumi dan Puti Bungsu merupakan manusia langit. Tari ini awalnya ditarian untuk menghibur Puti Bungsu yang berpisah dengan Malin Deman. Tari ini di tarian oleh beberapa penari perempuan secara berpasang-pasangan. Gerakan tari Gandai di ambil dari gerakan satwa yang mengambarkan kekecewaan malin deman yang ditinggal oleh Puti Bungsu. Pada saat ini tarian ini sudah berubah menjadi tari untuk hiburan dalam acara-acara perkawinan ataupun acara pesta rakyat di Kabupaten Mukomuko. Tari ini memiliki Sembilan gerak sesuai dengan lobang pada serunai gandai. Serunai gandai merupakan alat musik pengiring dalam tarian gandai. pada saat ini tari gandai sudah mengalami perubahan, baik dari segi bentuk tari maupun dari segi fungsi. Tujuan penelitian ini adalah untuk mengungkap bagaimana perubahan tari Gandai pada masyarakat Mukomuko Provinsi Bengkulu metode yang digunakan adalah metode kualitatif, pengumpulan data dilakukan melalui observasi dan mengamati tari Gandai tradisi dan kreasi.Kata Kunci: tari gandai, perubahan, Mukomuko.AbstractGandai dance is a traditional dance in mukomuko, Bengkulu province. This dance comes from the folklore Malin Deman and Puti Bungsu, Malin Deman is an earth man and the youngest Puti Bungsu is a celestial man. This dance was originaly danced to entertain Puti Bungsu who parted ways with Malin Deman. This dance is danced by several female dancers in pairs. The Gandai dance movement was taken from the animal movement which depicted the disappointment of Malin Deman who was left by the youngest Puti. At this time the dance has turned into a dance for entertainment in weddings or folk parties in Mukomuko District. This dance has nine movements inaccordance with the hole in yhe smart alley. The clever grun is a musical instrument accompaniment in smart dance. At this time the smart dance has undergone changes, both in terms of dance form and in terms of dance form and in terms of function. The purpose of this study was to reveal how the change of smart dance in the Bengkulu Province Mukomuko community method used was a qualitative method, data collection was carried out through observation and observing the traditional dance of tradition and creation. Keywords: gandai’s dance, change, Mukomuko.