bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ursolic acid reduces Adriamycin resistance of human ovarian cancer cells through promoting the HuR translocation from cytoplasm to nucleus
Authors: Li Wei , Luo Lanlan , Shi Wenyin , Yin Yujun , Gao Shan 
Published: October 2020

AbstractUrsolic acid (UA) has been shown to suppress various tumor progression, however, its roles in Adriamycin resistance of human ovarian cancer (OC) cells are still unclear. This work aims to investigate the effects of UA on the Adriamycin resistance of human OC cells. Here, we constructed Adriamycin‐resistant OC SKOV3‐Adr cells and found that UA attenuated Adriamycin resistance in SKOV3‐Adr cells. Additionally, UA enhanced Adriamycin sensitivity in the parental SKOV3 and another OC cell line A2780 cells. Mechanistic studies showed that HuR mRNA level was similar between SKOV3 and SKOV3‐Adr cells, but the cytoplasmic expression of HuR protein was increased in SKOV3‐Adr cells compared with that in SKOV3 cells, and subsequently enhancing the mRNA stability of multidrug resistance gene 1 (MDR1). Moreover, UA had no effects on HuR expression, but promoted the cytoplasm‐nucleus translocation of HuR protein, decreased MDR1 mRNA stability and thus reduced MDR1 expression. Furthermore, overexpression of MDR1 rescued the effects of UA on Adriamycin resistance and sensitivity. This work reveals a novel HuR/MDR1 axis responsible for UA‐mediated attenuation on Adriamycin resistance in OC cells.
Steel sheets partnered with quenchable sheet in hot stamping of tailor-welded blanks and its application to separation prevention of fractured components
Authors: Mori Ken-ichiro , Suzuki Yasutaka , Yokoo Daisuke , Nishikata Michiya , Abe Yohei 
Published: October 2020

AbstractThe phase transformation and mechanical properties of non-quenchable steels partnered with the quenchable boron steel in hot stamping of tailor-welded blanks were evaluated to produce tailored components with partially balanced strength and ductility. The effect of the forming start temperature after natural air cooling on the phase transformation and mechanical properties for 270 MPa mild steel, non-quenchable steel, 440 MPa high strength steel, and 22MnB5 steel sheets was examined, and the 270 MPa and non-quenchable sheets had enough ductility after hot stamping. Tailored components having a hardness of about 500 HV1 in the high strength zone and a total elongation of about 30% in the high ductility zone were hot-stamped from a tailor-welded blank composed of 22MnB5 and 270 MPa sheets. It was found that the 270 MPa mild steel sheet is sufficient as a partner sheet of tailor-welded blanks. In addition, the safety of hot-stamped components was heightened by welding a 22MnB5 main blank with a 270 MPa steel patch. Even if the main blank is fractured by a collision, the hot-stamped component is not separated by the 270 MPa patch having high ductility.
Decay of Hamiltonian Breathers Under Dissipation
Authors: Eckmann Jean-Pierre , Wayne C. Eugene 
Published: October 2020

AbstractWe study metastable behavior in a discrete nonlinear Schrödinger equation from the viewpoint of Hamiltonian systems theory. When there are $$n<\infty $$

n
<
∞

sites in this equation, we consider initial conditions in which almost all the energy is concentrated in one end of the system. We are interested in understanding how energy flows through the system, so we add a dissipation of size $$\gamma $$
γ
at the opposite end of the chain, and we show that the energy decreases extremely slowly. Furthermore, the motion is localized in the phase space near a family of breather solutions for the undamped system. We give rigorous, asymptotic estimates for the rate of evolution along the family of breathers and the width of the neighborhood within which the trajectory is confined.
Early posterior negativity in humans to pictures of snakes and spiders: effects of proximity
Authors: Beligiannis Nick , Van Strien Jan W. 
Published: October 2020

AbstractSnakes have proven to drive early attentional capture due to their evolutionary importance, as reflected by the early posterior negativity (EPN). The EPN snake effect might be partly driven by the proximity of the animal. In this study, by employing full-body (medium shot) and head-focused (close-up) pictures, we investigated whether the relative nearness (proximity) of the animal on the picture affects the snake EPN effect. We presented thirty participants with medium shot and close-up snake, spider and bird pictures in a rapid serial presentation paradigm at a presentation rate of three frames per second. We extracted the mean EPN activity from the 225–330 ms time frame after stimulus onset at the parietal–occipital cluster (PO3, O1, Oz, O2, PO4). The results indicate enhanced EPN for snake pictures as compared to spider and bird pictures. In addition, medium-shot snake pictures elicited higher EPN amplitudes than close-up snake pictures, suggesting that the EPN is higher when local, high spatial frequency attributes are visible. Spatial frequency analysis of the stimuli indicated that medium-shot snake pictures possess more power in the high spatial frequency bands, compared to medium-shot spider and bird pictures.
Quality of anticholinergic burden scales and their impact on clinical outcomes: a systematic review
Authors: Lisibach Angela , Benelli Valérie , Ceppi Marco Giacomo , Waldner-Knogler Karin , Csajka Chantal , Lutters Monika 
Published: October 2020

Abstract
Purpose
Older people are at risk of anticholinergic side effects due to changes affecting drug elimination and higher sensitivity to drug’s side effects. Anticholinergic burden scales (ABS) were developed to quantify the anticholinergic drug burden (ADB). We aim to identify all published ABS, to compare them systematically and to evaluate their associations with clinical outcomes.

Methods
We conducted a literature search in MEDLINE and EMBASE to identify all published ABS and a Web of Science citation (WoS) analysis to track validation studies implying clinical outcomes. Quality of the ABS was assessed using an adapted AGREE II tool. For the validation studies, we used the Newcastle-Ottawa Scale and the Cochrane tool Rob2.0. The validation studies were categorized into six evidence levels based on the propositions of the Oxford Center for Evidence-Based Medicine with respect to their quality. At least two researchers independently performed screening and quality assessments.

Results
Out of 1297 records, we identified 19 ABS and 104 validations studies. Despite differences in quality, all ABS were recommended for use. The anticholinergic cognitive burden (ACB) scale and the German anticholinergic burden scale (GABS) achieved the highest percentage in quality. Most ABS are validated, yet validation studies for newer scales are lacking. Only two studies compared eight ABS simultaneously. The four most investigated clinical outcomes delirium, cognition, mortality and falls showed contradicting results.

Conclusion
There is need for good quality validation studies comparing multiple scales to define the best scale and to conduct a meta-analysis for the assessment of their clinical impact.
Short- and Long-term Outcome After Interventional VSD Closure: A Single-Center Experience in Pediatric and Adult Patients
Authors: Bergmann M. , Germann C. P. , Nordmeyer J. , Peters B. , Berger F. , Schubert S. 
Published: October 2020

AbstractInterventional closure of congenital ventricular septal defects (VSD) is recording a continuous rise in acceptance. Complete atrioventricular block (cAVB) and residual shunting are major concerns during follow-up, but long-term data for both are still limited. We retrospectively evaluated the outcome of patients with interventional VSD closure and focused on long-term results (> 1 year follow-up). Transcatheter VSD closures were performed between 1993 and 2015, in 149 patients requiring 155 procedures (104 perimembranous, 29 muscular, 19 residual post-surgical VSDs, and 3 with multiple defects). The following devices were used: 65 × Amplatzer™Membranous VSD Occluder, 33 × Duct Occluder II, 27 × Muscular VSD Occluder, 3 × Duct Occluder I, 24 × PFM-Nit-Occlud®, and 3 × Rashkind-Occluder. The median age at time of implantation was 6.2 (0.01–66.1) years, median height 117 (49–188) cm, and median weight 20.9 (3.2–117) kg. Median follow-up time was 6.2 (1.1–21.3) years and closure rate was 86.2% at last follow-up. Complications resulting in device explantation include one case of cAVB with a Membranous VSD occluder 7 days after implantation and four cases due to residual shunt/malposition. Six (4%) deaths occurred during follow-up with only one procedural related death from a hybrid VSD closure. Overall, our reported results of interventional VSD closure show favorable outcomes with only one (0.7%) episode of cAVB. Interventional closure offers a good alternative to surgical closure and shows improved performance by using softer devices. However, prospective long-term data in the current era with different devices are still mandatory to assess the effectiveness and safety of this procedure.
Increased pulmonary serotonin transporter in patients with chronic obstructive pulmonary disease who developed pulmonary hypertension
Authors: Frille Armin , Rullmann Michael , Becker Georg-Alexander , Patt Marianne , Luthardt Julia , Tiepolt Solveig , Wirtz Hubert , Sabri Osama , Hesse Swen , Seyfarth Hans-Juergen 
Published: October 2020

Abstract
Purpose
Pulmonary hypertension (PH) is characterized by a progressive remodelling of the pulmonary vasculature resulting in right heart failure and eventually death. The serotonin transporter (SERT) may be involved in the pathogenesis of PH in patients with chronic-obstructive pulmonary disease (COPD). This study investigated for the first time the SERT in vivo availability in the lungs of patients with COPD and PH (COPD+PH).

Methods
SERT availability was assessed using SERT-selective [11C]DASB and positron emission tomography/computed tomography (PET/CT) with dynamic acquisition over 30 min in 4 groups of 5 participants each: COPD, COPD+PH, pulmonary arterial hypertension, and a healthy control (HC). Time activity curves were generated based on a volume of interest within the middle lobe. Tissue-to-blood concentration ratios after 25 to 30 min (TTBR25–30) served as receptor parameter for group comparison and were corrected for lung tissue attenuation. Participants underwent comprehensive pulmonary workup. Statistical analysis included group comparisons and correlation analysis.

Results
[11C]DASB uptake peak values did not differ among the cohorts after adjusting for lung tissue attenuation, suggesting equal radiotracer delivery. Both the COPD and COPD+PH cohort showed significantly lower TTBR25–30 values after correction for lung attenuation than HC. Attenuation corrected TTBR25–30 values were significantly higher in the COPD+PH cohort than those in the COPD cohort and higher in non-smokers than in smokers. They positively correlated with invasively measured severity of PH and inversely with airflow limitation and emphysema. Considering all COPD patients ± PH, they positively correlated with right heart strain (NT-proBNP).

Conclusion
By applying [11C]DASB and PET/CT, semiquantitative measures of SERT availability are demonstrated in the lung vasculature of patients with COPD and/or PH. COPD patients who developed PH show increased pulmonary [11C]DASB uptake compared to COPD patients without PH indicating an implication of pulmonary SERT in the development of PH in COPD patients.
Successful management of hyperammonemia with
hemodialysis on day 2 during 5-fluorouracil treatment in a patient with gastric cancer: a
case report with 5-fluorouracil metabolite analyses
Authors: Ozaki Yoshinao , Imamaki Hirotaka , Ikeda Aki , Oura Mitsuaki , Nakagawa Shunsaku , Funakoshi Taro , Kataoka Shigeki , Nishikawa Yoshitaka , Horimatsu Takahiro , Yonezawa Atsushi , Matsubara Takeshi , Yanagita Motoko , Muto Manabu , Watanabe Norihiko 
Published: October 2020

Abstract
Purpose
Hyperammonemia is an important adverse event associated with
5-fluorouracil (5FU) from 5FU metabolite accumulation. We present a case of an
advanced gastric cancer patient with chronic renal failure, who was treated with
5FU/leucovorin (LV) infusion chemotherapy (2-h infusion of LV and 5FU bolus followed
by 46-h 5FU continuous infusion on day 1; repeated every 2 weeks) and developed
hyperammonemia, with the aim of exploring an appropriate hemodialysis (HD) schedule
to resolve its symptoms.

Methods
The blood concentrations of 5FU and its metabolites, α-fluoro-β-alanine
(FBAL), and monofluoroacetate (FA) of a patient who had hyperammonemia from seven
courses of palliative 5FU/LV therapy for gastric cancer were measured by liquid
chromatography–mass spectrometry.

Results
On the third day of the first cycle, the patient presented with
symptomatic hyperammonemia relieved by emergency HD. Thereafter, the 5FU dose was
reduced; however, in cycles 2–4, the patient developed symptomatic hyperammonemia and
underwent HD on day 3 for hyperammonemia management. In cycles 5–7, the timing of
scheduled HD administration was changed from day 3 to day 2, preventing symptomatic
hyperammonemia. The maximum ammonia and 5FU metabolite levels were significantly
lower in cycles 5–7 than in cycles 2–4 (NH3 75 ± 38 vs 303 ± 119 μg/dL, FBAL
13.7 ± 2.5 vs 19.7 ± 2.0 μg/mL, FA 204.0 ± 91.6 vs 395.9 ± 12.6 ng/mL,
mean ± standard deviation, all p < 0.05). After
seven cycles, partial response was confirmed.

Conclusion
HD on day 2 instead of 3 may prevent hyperammonemia in 5FU/LV
therapy.
Impact of depressive disorders on quality of life after middle ear surgery in patients with chronic otitis media
Authors: Lailach Susen , Langanke Theresa , Zahnert Thomas , Garthus-Niegel Susan , Neudert Marcus 
Published: October 2020

Abstract
Purpose
The aim of this study was to determine whether preoperative depressive symptoms influence health-related quality of life (HRQOL) after middle ear surgery in patients with chronic otitis media (COM).

Methods
This prospective clinical case study was conducted at a tertiary referral center. All 102 patients who had undergone middle ear surgery for COM were assessed clinically and by audiometric testing (pure tone audiometry) in pre- and postoperative settings. Disease-specific HRQOL was assessed by the validated chronic otitis media outcome test 15 (COMOT-15) and the Zurich chronic middle ear inventory (ZCMEI-21). General HRQOL was measured using the short form 36 (SF-36). Depressive symptoms were assessed using the patient health questionnaire (PHQ-D). The Charlson comorbidity index (CCI) was used to classify comorbidities. The middle ear status was determined using the ossiculoplasty outcome parameter staging (OOPS) index.

Results
After middle ear surgery, the total COMOT-15 and ZCMEI-21 scores improved significantly (p < 0.001). General HRQOL (total SF-36 score) was unaffected by surgery (p < 0.05). Patients without elevated depressive symptoms had significantly better total scores for the COMOT-15 (p < 0.01), ZCMEI-21 (p < 0.001), and for SF-36 (p < 0.001) postoperatively. The results of the multiple regression analyses show that, after adjusting for the OOPS, CCI, and hearing improvement, preoperative depressiveness was significantly associated with worse postoperative COMOT-15 and ZCMEI-21 outcome scores (β = 0.425 and β = 0.362, p < 0.001).

Conclusion
Preoperative depressiveness was an essential predictive factor for HRQOL in patients with COM. This should be considered during patient selection to provide more suitable preoperative counseling.
Variant analysis of the sporozoite surface antigen gene reveals that asymptomatic cattle from wildlife-livestock interface areas in northern Tanzania harbour buffalo-derived T. parva
Authors: Mwamuye Micky M. , Odongo David , Kazungu Yvette , Kindoro Fatuma , Gwakisa Paul , Bishop Richard P. , Nijhof Ard M. , Obara Isaiah 
Published: October 2020

AbstractBuffalo-derived Theileria parva can ‘break through’ the immunity induced by the infection and treatment vaccination method (ITM) in cattle. However, no such ‘breakthroughs’ have been reported in northern Tanzania where there has been long and widespread ITM use in pastoralist cattle, and the Cape buffalo (Syncerus caffer) is also present. We studied the exposure of vaccinated and unvaccinated cattle in northern Tanzania to buffalo-derived T. parva using p67 gene polymorphisms and compared this to its distribution in vaccinated cattle exposed to buffalo-derived T. parva in central Kenya, where vaccine ‘breakthroughs’ have been reported. Additionally, we analysed the CD8+ T cell target antigen Tp2 for positive selection. Our results showed that 10% of the p67 sequences from Tanzanian cattle (n = 39) had a buffalo type p67 (allele 4), an allele that is rare among East African isolates studied so far. The percentage of buffalo-derived p67 alleles observed in Kenyan cattle comprised 19% of the parasites (n = 36), with two different p67 alleles (2 and 3) of presumptive buffalo origin. The Tp2 protein was generally conserved with only three Tp2 variants from Tanzania (n = 33) and five from Kenya (n = 40). Two Tanzanian Tp2 variants and two Kenyan Tp2 variants were identical to variants present in the trivalent Muguga vaccine. Tp2 evolutionary analysis did not show evidence for positive selection within previously mapped epitope coding sites. The p67 data indicates that some ITM-vaccinated cattle are protected against disease induced by a buffalo-derived T. parva challenge in northern Tanzania and suggests that the parasite genotype may represent one factor explaining this.
A Flow Perspective on Nonlinear Least-Squares Problems
Authors: Bock Hans Georg , Gutekunst Jürgen , Potschka Andreas , Garcés María Elena Suaréz 
Published: October 2020

AbstractJust as the damped Newton method for the numerical solution of nonlinear algebraic problems can be interpreted as a forward Euler timestepping on the Newton flow equations, the damped Gauß–Newton method for nonlinear least squares problems is equivalent to forward Euler timestepping on the corresponding Gauß–Newton flow equations. We highlight the advantages of the Gauß–Newton flow and the Gauß–Newton method from a statistical and a numerical perspective in comparison with the Newton method, steepest descent, and the Levenberg–Marquardt method, which are respectively equivalent to Newton flow forward Euler, gradient flow forward Euler, and gradient flow backward Euler. We finally show an unconditional descent property for a generalized Gauß–Newton flow, which is linked to Krylov–Gauß–Newton methods for large-scale nonlinear least squares problems. We provide numerical results for large-scale problems: An academic generalized Rosenbrock function and a real-world bundle adjustment problem from 3D reconstruction based on 2D images.
Depletion of groundwater resources under rapid urbanisation in Africa: recent and future trends in the Nairobi Aquifer System, Kenya
Authors: Oiro Samson , Comte Jean-Christophe , Soulsby Chris , MacDonald Alan , Mwakamba Canute 
Published: October 2020

AbstractThe Nairobi volcano-sedimentary regional aquifer system (NAS) of Kenya hosts >6 M people, including 4.7 M people in the city of Nairobi. This work combines analysis of multi-decadal in-situ water-level data with numerical groundwater modelling to provide an assessment of the past and likely future evolution of Nairobi’s groundwater resources. Since the mid-1970s, groundwater abstraction has increased 10-fold at a rate similar to urban population growth, groundwater levels have declined at a median rate of 6 m/decade underneath Nairobi since 1950, whilst built-up areas have increased by 70% since 2000. Despite the absence of significant trends in climatic data since the 1970s, more recently, drought conditions have resulted in increased applications for borehole licences. Based on a new conceptual understanding of the NAS (including insights from geophysics and stable isotopes), numerical simulations provide further quantitative estimates of the accelerating negative impact of abstraction and capture the historical groundwater levels quite well. Analysis suggests a groundwater-level decline of 4 m on average over the entire aquifer area and up to 46 m below Nairobi, net groundwater storage loss of 1.5 billion m3and 9% river baseflow reduction since 1950. Given current practices and trajectories, these figures are predicted to increase six-fold by 2120. Modelled future management scenarios suggest that future groundwater abstraction required to meet Nairobi projected water demand is unsustainable and that the regional anthropogenically-driven depletion trend can be partially mitigated through conjunctive water use. The presented approach can inform groundwater assessment for other major African cities undergoing similar rapid groundwater development.
Comparing navigated transcranial magnetic stimulation mapping and “gold standard” direct cortical stimulation mapping in neurosurgery: a systematic review
Authors: Jeltema Hanne-Rinck , Ohlerth Ann-Katrin , de Wit Aranka , Wagemakers Michiel , Rofes Adrià , Bastiaanse Roelien , Drost Gea 
Published: October 2020

AbstractThe objective of this systematic review is to create an overview of the literature on the comparison of navigated transcranial magnetic stimulation (nTMS) as a mapping tool to the current gold standard, which is (intraoperative) direct cortical stimulation (DCS) mapping. A search in the databases of PubMed, EMBASE, and Web of Science was performed. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and recommendations were used. Thirty-five publications were included in the review, describing a total of 552 patients. All studies concerned either mapping of motor or language function. No comparative data for nTMS and DCS for other neurological functions were found. For motor mapping, the distances between the cortical representation of the different muscle groups identified by nTMS and DCS varied between 2 and 16 mm. Regarding mapping of language function, solely an object naming task was performed in the comparative studies on nTMS and DCS. Sensitivity and specificity ranged from 10 to 100% and 13.3–98%, respectively, when nTMS language mapping was compared with DCS mapping. The positive predictive value (PPV) and negative predictive value (NPV) ranged from 17 to 75% and 57–100% respectively. The available evidence for nTMS as a mapping modality for motor and language function is discussed.
Feasibility of Co-Treating Olive Mill Wastewater and Acid Mine Drainage
Authors: Carlier Jorge Dias , Luís Ana Teresa , Alexandre Luís Miguel , Costa Maria Clara 
Published: October 2020

AbstractPrevious tests using a growth medium and olive mill wastewater (OMWW) have shown that it supplies carbon and electron donors suitable for sulphate reducing bacteria (SRB). We assessed the co-treatment of acid mine drainage (AMD) and OMWW using SRB-enriched bioreactors and identified the most abundant bacterial populations present under optimized conditions. The process requires a neutralizing agent to create optimal pH conditions for successful removal of the AMD’s main contaminants. Concentrations of SO42−, Al, Fe, Cu, Zn, and Mn decreased to below Portugal’s maximum admissible values for irrigation waters, and all but Mn were reduced to less than Portugal’s emission limit values (ELVs) for wastewater discharges. Phenol concentrations—the main pollutants in OMWW—dropped to values between 1/10 and 1/5 their initial concentrations in batch tests using mixtures of AMD and OMWW, and to 1/2 their initial concentrations in flow-through tests. The final total phenol concentrations were still above the ELV for wastewater discharges, but phenols are not regulated in irrigation waters, and OMWW is used by some producers to irrigate soils. Six main SRB groups were identified as likely having a fundamental role in the bioremediation process: the genera Desulfovibrio, Sulfurospirillum, and Acetobacter and the families Sphingomonadaceae, Prevotellaceae, and Deferribacteraceae.