bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimating Sector-Specific Data of the Electric Power Industry to Analyze the Effect of the Liberalization: The Case of Japan
Authors: Wakashiro Yasunobu 
Published: August 2020

Abstract
Input-output tables are employed to analyze the liberalization of electricity industries around the world. However, the input-output tables do not have sectoral data of the electricity industry in many countries. Electricity industries that consist of an electricity generation, transmission, and retail (and/or distribution) sector have experienced the different degrees of liberalization; the generation and retail sector have experienced liberalization in many countries or regions, while the transmission sector has not faced liberalization. In this study, we estimate the sectoral data of the electricity industry in an input-output table by using annual reports of the electric power companies and also defined the relationship among the sectors. Finally, we find that the data which are analyzed by previous methods can distort policy decisions and that especially the definition of the sectors' intersection in the industry is critical.Category & Number: 5. Industrial Organization and Structural PolicyJEL Classification: C32, C33, Q41, Q48
TER94 , the Drosophila Homologue of the ALS-related VCP gene, Influences Lifespan and Leads to a Decline in Motor Function
Authors: Hurley Emily Priscilla , Staveley Brian Ernest 
Published: August 2020

Abstract
Background: Valosin-Containing Protein (VCP) is an essential AAA+ ATPase with diverse functions within the cell. Mutations in the VCP gene have been detected in patients with familial amyotrophic lateral sclerosis (ALS). The aim of this study is to create a novel model of human neurodegenerative disease in Drosophila melanogaster by altering the expression of TER94, the Drosophila orthologue of the human VCP gene. TER94 expression was altered in all neurons, the dopaminergic neurons and in the motor neurons, with longevity and locomotor function assessed over time. Altered TER94 expression in combination with the altered expression of known Parkinson Disease (PD) genes was examined to investigate potential interactions.Results: Inhibition of TER94 altered median lifespan in a manner dependent upon the transgene selected for use and the tissue-specific expression directed by the Gal4 transgene selected. Locomotor ability was significantly reduced in all cases of TER94 inhibition tested. The inhibition of TER94 by two TER94-RNAi inhibitory transgenes, in the motor neurons via D42-Gal4 lead to increases in median lifespan, with one inhibitory transgene generating a slightly reduced lifespan. Inhibition of TER94 in the dopaminergic neurons resulted in a severe reduction in lifespan. The co-inhibition of TER94 and parkin in the neurons resulted in a major decline in lifespan by approximately 30%. While the inhibition of TER94 and the co-expression of alpha-synuclein in the neurons resulted in an increase in lifespan by approximately 28%. Conclusions: The inhibition of TER94 in the motor neurons is an interesting model of ALS, due to the small, but reduced lifespan coupled with a strong decline in locomotor function. The inhibition of TER94 in the dopaminergic neurons is a potential model of ALS, due to the reduction of both lifespan and locomotor function over time. The co-inhibition of TER94 and parkin in the neurons provides a promising novel model of neurodegenerative disease, displaying a great reduction in lifespan and in locomotor ability over time.
Clinical Observation and Analysis of Patients with Idiopathic Membranous Nephropathy Combined with Low 25(OH) D3 Levels
Authors: Zou Fan , Huang Yu , Yang Zhenhua 
Published: August 2020

Abstract
Background: To investigate the correlations between 25-hydroxyvitamin D3 [25(OH) D3] levels and selected clinical indicators and renal pathology at initial onset of idiopathic membranous nephropathy (IMN) and to analyze changes in the main quantifiable indicators in IMN patients with different 25(OH) D3 levels.Methods: A total of 109 patients admitted to the Department of Nephrology of the First Affiliated Hospital of Guangxi Medical University between August 2015 and April 2019 and diagnosed with IMN were reviewed. Comparative analysis of the effects of different 25(OH)D3 levels on clinical and pathological indicators of IMN was performed, and the changes in serum albumin, eGFR, serum creatinine and other clinical indicators in these patients during follow-up were analyzed.Results: The 109 patients with IMN had 25(OH)D3 deficiency [25(OH)D3<70 nmol/L]. The incidence of nephrotic syndrome, serum phosphorus level, hs-CRP level, and ESR were higher in the 25(OH)D3<25nmol/L group than in the 25(OH)D3≥25nmol/L group. In contrast, the TP, ALB, serum calcium, and IgG levels were lower in the 25(OH)D3<25nmol/L group (P<0.05). No pathological parameters differed significantly between the two groups (P>0.05). Bivariate correlation analysis showed that 25(OH)D3 was positively correlated with nephrotic syndrome, the TP level, the ALB level, the eGFR, the serum calcium level and the IgG level (P<0.05) and negatively correlated with age, blood pressure, the Scr level, 24-hour UP quantitation, the serum phosphorus level, the hs-CRP level and the ESR (P<0.05). In single-factor repeated measures analysis of variance, the ALB levels in the 25(OH)D3<25nmol/L and 25(OH)D3≥25nmol/L groups showed an increasing trend but were lower in the 25(OH)D3<25nmol/L group than in the 25(OH)D3≥25 nmol/L group at each follow-up time point (P<0.001).Conclusion: Reduced 25(OH)D3 levels are prevalent among IMN patients. During follow-up, serum albumin increased significantly in patients administered vitamin D supplementation. 25(OH)D3 may play an important role in IMN development.
The Association Between Self-Management Ability and Malnutrition-Inflammation-Atherosclerosis Syndrome in Peritoneal Dialysis Patients: A Cross-Sectional Study
Authors: Huang Zehui , Fang Junyang , Song Ahui , Tong Yan , Deng Hai , Wei Shan , Ji Ouyang , Hu Chun , Li Pu , Zhang Chunli , Liu Yingli 
Published: August 2020

Abstract
BackgroundThe relationship between malnutrition-inflammation-atherosclerosis syndrome (MIAS) and self-management ability has not been previously revealed even though both play an important role in the management of peritoneal dialysis (PD) patients.Materials and methodsIn total, 93 patients were enrolled in this study. A self-management questionnaire was used for the evaluation of self-management ability. The identification of MIAS was based on one or more of the following three conditions: C-reactive protein (CRP) ≥10 mg/L, malnutrition–inflammation score (MIS) > 7, and the presence of atherosclerosis related medical records. The possible association between different self-management abilities and MIAS was analyzed with a Spearman correlation analysis.ResultsThere were 40 (43.0%) patients in the atherosclerosis group, and 38 (40.9%), 38 (40.9%), 10 (10.8%), and 7 (7.5%) patients in the MIAS0, MIAS1, MIAS2, and MIAS3 groups, respectively. The group with a score above the mean score of the Dialysis Effect Evaluation and Monitoring dimension had a fewer number of hospitalizations, higher albumin levels, lower MIS scores, lower level of IL-6, and a lower number of MIAS factors. The Pearson and Spearman correlation analyses also revealed that this dimension was negatively correlated with the MIAS, MIS, IL-6, BNP, number of hospitalizations, and age and positively associated with albumin and prealbumin. ConclusionThe Dialysis Effect Evaluation and Monitoring dimension of the self-management scale for PD patients is closely linked to the MIAS, and the better the dialysis effect evaluation and monitoring capacity, the lower the chance of exposure to malnutrition and inflammation.Trial registrationChina Clinical Trials Register: SH9H-2020-T20-2, retrospectively registered 15 January 2020.
First Insights Into the Molecular Basis Association Between Promoter Polymorphisms of the Il1b Gene and Helicobacter Pylori Infection in the Sudanese Population: Computational Approach
Authors: Idris Abeer Babiker , Idris Einas Babiker , Ataelmanan Amany Eltayib , Mohamed Ali Elbagir Ali , Arbab Bashir M. Osman , Ibrahim El-Amin Mohamed , Hassan Mohamed A. 
Published: August 2020

Abstract
Background:Helicobacter pylori (H. pylori) infects nearly half of the world’s population with a variation in incidence among different geographic regions. Genetic variants in the promoter regions of IL1B gene can affect cytokine expression and creates a condition of hypoacidity which favors the survival and colonization of H. pylori.Aim: To characterize the polymorphic sites in the 5’- region [-687_+297] of IL1B in H. pylori infection using in silico tools.Methods: Genomic DNA was extracted from 121 gastric biopsies and genotyping of IL1B-31 polymorphism was performed using PCR-CTPP to investigate its association with the susceptibility to H. pylori infection in the Sudanese population. In addition, Sanger sequencing was applied to detect SNPs in the 5’-region [-687_+297] of IL1B in 14 H. pylori-infected patients; and bioinformatics analyses were used to predict whether these mutations would alter TFBSs or CEs in this region. Also, comparative analysis was conducted for this region in 11 species using ECR browser and Mulan search engine.Results: A total of five nucleotide variations were detected in the 5’-regulatory region which led to the addition or alteration of the TFBSs and CEs. Genotyping of IL1B-31 C>T revealed a significant association between -31T and susceptibility to H. pylori infection in the studied population (P=0.0280). Comparative analysis showed conservation rates of IL1B upstream [−368_+10] region above 70% in chimpanzee, rhesus monkey, a domesticated dog, cow and rat.Conclusion: In H. pylori-infected patients, three detected SNPs (-338, -155 and -31) located in the IL1B promoter were predicted to alter TFBSs and CE, thereby might affecting gene expression. These in silico predictions provide insight for further experimental in vitro and in vivo studies of the regulation of IL1B expression and its relationship to H. pylori infection. However, recognition of regulatory motifs by computer algorithms is fundamental for understanding gene expression patterns.
Intestinal CD4-CD8αβ-TCRαβ+ T cells function as tolerogenic antigen presenting cells
Authors: Nemoto Yasuhiro , Tanaka Shohei , Takei Yuria , Morikawa Ryo , Oshima Shigeru , Nagaishi Takashi , Tsuchiya Kiichiro , Nakamura Tetsuya , Takenaka Kento , Ohtsuka Kazuo , Chen Xigui , Okazawa Hitoshi , Okamoto Ryuichi , Watanabe Mamoru , Andrian Ulrich von 
Published: August 2020

Abstract
The intestinal immune system is constantly exposed to a plethora of antigens (Ags) from innocuous ingested material and the commensal flora that must be distinguished from pathogen-derived antigens. To this end, a number of anatomic, cellular and molecular mechanisms operate in the intestinal tract to acquire, process, present and interpret Ags from the intestinal lumen. The intestinal mucosa is also populated by a large number of T cells that reside within the epithelial lining (intraepithelial lymphocytes, IELs), in the underlying lamina propria (LPLs) and in gut-associated lymphoid tissues (GALT). Both IELs and LPLs are heterogeneous populations consisting of conventional CD4+ and CD8+ T cells and numerous unconventional T cells, including TCRγδ+ T cells and CD4-CD8αβ-TCRαβ+T cells (double negative; DNT cells). The latter are rarely found in non-intestinal tissues and their function is still enigmatic. Here, we show that murine DNT cells in the small intestine (SI) reach across the epithelial barrier to capture luminal Ags. A sizeable fraction of DNT cells in Peyer’s patches (PPs), mesenteric lymph nodes (MLNs) and among LPLs, but not among IELs, express MHC-II, but little or no classical co-stimulatory molecules, suggesting that DNT cells-mediated Ag presentation to naïve CD4+ T cells may trigger tolerogenic rather than effector responses. Indeed, intestinal DNT cells, particularly in PPs, acquire, process and present antigenic proteins and tolerize Ag-specific CD4+ T cells. Conditional genetic ablation of MHC-II in T cells disabled this suppressive function and rendered mutant mice hypersusceptible to DSS colitis. Moreover, similar to our findings in mice, DNT cells in human SI express HLA-DR and readily uptake exogenous Ags. Intriguingly, intestinal DNT cells in Crohn’s disease patients expressed lower levels of HLA-DR than control patients. These findings suggest that MHC-II+ DNT cells play a key role in intestinal immune homeostasis and may contribute to the pathogenesis of inflammatory bowel disease.
Mapping the Vector Abundance of Endemic Mosquito-Borne Diseases in Southern Quebec
Authors: Allostry Julie , Ludwig Antoinette , Kotchi Serge Olivier , Rousseu François , Fournier Richard 
Published: August 2020

Abstract
Background: Climate change is increasing the dispersion of mosquitoes and the spread of viruses of which some mosquitoes are the main vectors. This increases the risk of humans coming into contact with infected mosquitoes and developing diseases with sometimes fatal consequences. In Quebec, the surveillance and management of endemic mosquito-borne diseases, such as West Nile virus or Eastern equine encephalitis, could be improved by mapping the areas of risk supporting vector populations. However, there is currently no active tool tailored to Quebec that can predict annual mosquito population abundances.Methods: Our modelling approach is designed to meet this need. Four species of mosquitos were studied in this project for the period from 2003 to 2014 for the southern part of the province: Aedes vexans (VEX), Coquillettidia perturbans (CQP), Culex pipiens-restuans group (CPRg) and Ochlerotatus stimulans group (STMg) species. We used a mixed linear regression approach to model the abundances of each species or species groups as a function of meteorological and land cover variables.Results: The best models incorporate, for CPRg, the agricultural land, grassland and woodland classes and the average minimum temperature in September of the previous year; for STMg, the urban and woodland classes and the mean precipitation in June; for CQP, urban areas and the mean precipitation in January and August; and finally, for VEX, the agricultural land class and the mean precipitation in January, February and September.Conclusions: The models proved to be robust and precise over almost the entire study area, and the presence of significant climate variables for each of the species or species groups makes it possible to consider their use in predicting long-term spatial variations, based on climate and landscape change, in the abundance of mosquitoes potentially harmful to public health in southern Quebec.Manuscript intended for publication in International J. of Health Geographics
RSV and exposure to cigarette smoking as risk factors for severe bronchiolitis in a tropical country
Authors: Buendia Jefferson A. , Acuña-Cordero Ranniery 
Published: August 2020

Abstract
Objetive: This study aims to identify risk factors associated with severe bronchiolitis in children. In a retrospective cohort study, we included all infants younger than two years of age in tertiary centers in Rionegro, Colombia, hospitalized due to bronchiolitis from January 2019 to December 2019. Severe bronchiolitis was defined as rhinorrhea, cough, tachypnea, wheezing, rales, and increased respiratory effort (e.g., grunting, nasal flaring, and intercostal and/or subcostal retraction), with symptoms of severity (e.g., increased respiratory rate, retractions, and oxygen saturation at 90% or lower). To identify factors independently associated with severe bronchiolitis, we used log-binomial regression and estimate prevalences ratio (PR) and adjust for potential confounding variables Results: Of a total of 417 included, 300 (72.12%) have severe bronchiolitis. After modeling and controlling for potential confounders in the multivariate analysis: RSV isolation (PR 1.15 95%CI 1.03–1.29) and current exposure to cigarette smoking (PR 1.19 95%CI 1.04–1.35) were independent predictors of severe bronchiolitis. We concluded that RSV and exposure to cigarette smoking are independent predictors of severe bronchiolitis. These potentially modifiable variables associated with severity should guide future interventions to reduce the morbidity and economic burden of bronchiolitis in our population.
Computational Gene Expression Profiling in Non-Small Cell Lung Cancer Reveals Network-Based Immune Signatures Associated With Smoking and Overall Survival
Authors: Song Rong-feng , Cheng Xiao-liang , Zhang Xin , Zhang Jun-kai , Fu Wen-fan , Li Kunsong 
Published: August 2020

Abstract
Background Lung cancer, a common risky disease, has been the leading cause of death since 2010. In this study, the prognosis of immune genes and its targeted miRNA of non-small lung cancer (NSCLC) was explored. Methods Based on microarray GSE13213 (lung adenocarcinoma (LAD) patient mRNA expression profiles), microarray GSE4573 (lung squamous cell carcinoma (LSC) patient mRNA expression profiles), and microarray GSE102286 (NSCLC patient miRNA profiles), the gene co-expression network was constructed with weight gene co-expression network analysis (WGCNA). KEGG and Gene Ontology were enriched. The binding site was predicted on miRanda. The genes corresponding to immune as well as smoking and its targeted miRNA which was also related to smoking was discovered. And then the prognostic value of them was estimated and confirmed in another data cohort. Results Smoking-related module from LSC had a remarkable association with immune gene set (P = 0.0001), which was not observed in LAD. KEGG and GO enrichment analysis in LSC also exhibited immunity-related pathways and functions, such as the B cell receptor signaling pathway, which was still not found in LAD. High expression of nine immune genes (CD27, CD38, CD79A, MZB1, IGHM, IGKC, IGLL3P, GUSBP11, and IGHD) from the purple module in LSC had better overall survival. In an independent LSC microarray, six of them (CD27, CD38, GUSBP11, IGKC, IGLL3P, and IGHD) were validated. IGHD related to plasma cell regulatory showed better overall survival (OS) in two datasets both. Low expression of IGHD targeted miR-29a and low infiltration of its regulated plasma cells was both associated with better OS. Conclusion Our study revealed that, in LSC, smoking might trigger a more severe immune response, compared to LAD. There were six immune genes related to prognosis being discovered. And miR-29a might bind to IGHD affect OS by regulating plasma cells in LSC.
Metagenomic and Metabonomic Profiling provide insights into AIDS resistance in African Green Monkey
Authors: Zhou Xiao-jun , Wang Jin , Shang Yan-jiao , Wang Shuang , Qiu Yefeng , Yuan Zheng 
Published: August 2020

Abstract
Background

As a natural host of simian immunodeficiency virus (SIV), African green monkeys (AGM) do not develop AIDS. AGM has recently been shown to rapidly activate and maintain evolutionarily conserved regenerative wound healing mechanisms in mucosal tissue in case of SIV acute infection, suggesting a role of mucosal integrity in AIDS resistance.However, little is known about the underlying mechanisms, especially of which the gut microbiome as well as the metabolites participate in. This study aims to characterize the profiles of gut microbiome and metabolites of AGM with chronic SIV infection (AGM_P), AGM without SIV infection (AGM_N), Cynomolgus macaque (CM), Rhesus macaque (RM).
Results

Compared with CM and RM, significant decreases in the abundances of Streptococcus, Alistipes, Treponema, Bacteoides, Methanobrevibacter, Methanobrevibacter (P < 0.01), as well as significant increases in the abundances of Clostridium, Eubacterium, Blautia, Roseburia, Faecalibacterium, Dialister (P < 0.01) were demonstrated in AGM_N. Compared with AGM_N, a trend in the increased abundances of Streptococcus and Roseburia were found in AGM_P. Quantitative RT-PCR analysis validated the increased abundances of butyrate-producing Faecalibacterium prausnitzii and Eubacteria in AGM_N (P < 0.05). Metabolites involved in lipid metabolism, as well as in butanoate metabolism, were found to be with significantly different levels between AGM_P, AGM_N and CM. And some of these metabolites were shown to be correlated to the abundances of Haloarchaeobius, Methanobrevibacter, Methanothermococcus, and Methanoregula in AGM_N and AGM_P.
Conclusions

Compared with CM and RM, AGM possesses different capacities of lipid metabolism and butanoate metabolism, which correlates with gut homeostasis and mucosal integrity. These findings may provide insight into AIDS resistance in AGM.
Screening the six plant species for phytoremediation of synthetic textile dye waste water
Authors: Kaur Navjeet , Kaushal Jyotsna , Mahajan Pooja , Srivas Arun Lal 
Published: August 2020

Abstract
Most of the dyes are carcinogenic and mutagenic in nature. Plants are potential candidates to remediate textile dye wastewater from contaminated sites. The present study aimed toscreen potential plant species for removal of synthetic dye solution of triarylmethane dye Methylene Blue (MB) and diazo dye Congo Red (CR). Six plant species were screened for their phytoremediation ability for the removal of dyes present in synthetic wastewater. Six plants selected for screening areTrachyspermum ammi L. (T. ammi), Tagetes erecta L. (T. erecta), Hibiscus rosa-sinensis L. (H. rosa- sinensis), Chrysanthemum indicum L. (C. indicum), Bryophyllum fedtschenkoi (B. fedtschenkoi), Catharanthus roseus L. (C. roseus). The phytotreatment of dyes was done up to 40 hfortwo different concentrations of dyes 10 mg L− 1 and 20 mg L− 1. Among these plants, the maximum decolouration was obtained from T. ammi plant followed by B. fedtschenkoi plant. Both of these plants showed active growth even after the phytoremediation process. T. ammi decolourised the MB dye 99% (10 mg L− 1) and 86% (20 mg L− 1) while the decolourisation of the CR dye solution was up to 95% (10 mg L− 1) and 84% (20 mg L− 1).T. ammi found to have maximum potential among screened plants for the removal of MB and CR dye from synthetic dye solution and can be used for decolouration of synthetic dye wastewater.
HDL Cholesterol Efflux Capacity and Concentration in Patients With Rheumatoid Arthritis: a Systematic Review and Meta-Analysis
Authors: Xie Binbin , He Jiang , Liu Yong , Liu Ting , Liu Chaoqun 
Published: August 2020

Abstract
Background: HDL cholesterol efflux capacity has been proposed as an independent risk factor of cardiovascular diseases. However, the presented data has been less than convincing in RA. This meta-analysis aimed to identify whether CEC was impaired in RA or altered by drug therapy.Methods: PubMed, Embase, and Cochrane library databases were searched to identify studies for CEC in RA patients. The primary outcomes were CEC, high-density lipoprotein cholesterol and C-reactive protein.Results: A total of 11 eligible articles including 6 observational and 5 intervention studies. Pooled results showed there was no significantly decrease in the CEC of RA patients comparing with the healthy controls (SMD: -0.22, 95% CI: -0.65 to 0.20), whereas the plasma HDL-C levels was decreased in RA patients (WMD: -5.21, 95% CI: -7.69 to -2.72). Furthermore, in the before-after studies, CEC of RA patients (SMD: 0.20, 95% CI: 0.03 to 0.38) was increased but the plasma HDL-C levels (WMD: 3.26, 95% CI: -0.17 to 6.69) was similar after anti-rheumatic treatment comparing to the baseline. In addition, stratified analysis suggested that DAS28 could be potential sources of heterogeneity for CEC. The funnel plot was relatively symmetric and was not suggestive of presence of publication bias.Conclusion The current meta-analysis demonstrated a slight, nonsignificant, decrease in the CEC among RA patients as compared with healthy individuals, but it can be improved substantially following anti-rheumatic treatment. More large population studies evaluating the predictive role of CEC on cardiovascular risk in RA are needed.