bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-Linear Relationships Between Maternal Pre-Pregnancy Body Mass Index, Gestational Weight Gain and Duration of Breastfeeding
Authors: Zhang Yijing , Lin Yanfen , Xu Jian , Luo Zhong-cheng , Cao Sirui , Liu Zhiwei , Huang Jun 
Published: August 2020

Abstract
Background:Considering the potential inappropriateness of the 2009 Institute-of-Medicine criteria to Chinese women, we investigated the associations between pre-pregnancy body-mass-index (BMI), gestational-weight-gain (GWG) and breastfeeding-duration among Chinese women.Methods:This birth-cohort study included 225 mother-child pairs in Shanghai from 2010 to 2012. Mothers were interviewed during mid-to-late pregnancy and at 24-36 months postpartum. Information on pre-pregnancy BMI/GWG and breastfeeding-duration was collected during pregnancy and at 24-36 months postpartum, respectively. Using a data-driven approach based on spline-smoothing-fitting of the data, two-piecewise linear regression models were used to assess the relations between pre-pregnancy BMI, GWG and breastfeeding-duration. Results:Mothers being younger, longer daily working hours, and less time living with children per week were associated with shorter duration (P<0.05). The "inverted-U"-shaped and "flat-then-declining"-shaped associations between pre-pregnancy BMI, GWG and breastfeeding-duration were observed, respectively. Adjusting for related confounders, the increases in pre-pregnancy BMI below and above 22.5 kg/m2 were associated with an increase (P=0.044) and a marginal decrease (P=0.077) in breastfeeding-duration, respectively. One-kilogram increase in GWG was associated with 0.4-month decrease (95% CI: -0.7, -0.1) in breastfeeding-duration when GWG ≥ 17.5 kilograms, however, with nonsignificant changes when GWG <17.5 kilograms. Conclusion:Appropriate pre-pregnancy BMI (around 22.5 kg/m2) and GWG (< 17.5 kilograms) were favorable to sustained breastfeeding.
Evidence for ABL Amplification in Multiple Myeloma and Its Role in Treatment
Authors: Huang He , Guo Wenjian , He Licai , Yao Ronxin , Lin Ying , Yu Kang , Li Qian 
Published: August 2020

Abstract
Background: The cytogenetic abnormalities are considered as initiating events in the pathogenesis of multiple myeloma (MM) and were assumed to be clinical significance. A number of defined cytogenetic lesions have been reported by genetic analysis techniques, while ABL gene, known as the therapeutic target in chronic myelogenous leukemia (CML), its expression in myeloma has not been deeply explored.Methods: We used publically available method FISH to analyze the chromosomal architecture, clinic features and overall survival of 101 MM patient samples. Additionally, we examined ABL expression in MM cell lines (NCI-H929, LP-1 and U266) through FISH and western blot. After culturing with ABL kinase inhibitor STI571, we analyzed MM cell proliferation by CCK8 assay and detected ABL protein levels by western blot.Results: Together with reported chromosomal abnormalities, we found ABL gene exhibited not as BCR-ABL fusion gene in CML, but its amplification was prevalent, 67 patients (66.3%) had cytogenetic abnormalities with ABL amplification. And the patients with ABL gene amplification indicates no significance with clinical features, adverse cytogenetics (C-MYC amplification, IGH rearrangement, RB1 deletion, P53 deletion and 1q21 amplification) and overall survival comparing to patients with normal ABL expression. Moreover, we revealed ABL amplification in MM cell lines (LP-1 and U266) by FISH, and ABL protein was easy to detect in MM cell lines and some tumor cells. According to CML cells, the cytotoxicity of STI571to MM cells was definitely limited.Conclusions: Our study first discussed ABL gene amplification in MM cells, and we believe ABL gene would potentially be a useful target in the treatment of combination strategy for MM with ABL amplification in future.
circVAR Database: Genome-Wide Archive of Genetic Variants for Human Circular RNAs
Authors: Zhao Min , Qu Hong 
Published: August 2020

Abstract
Background: Circular RNAs (circRNAs) play important roles in regulating gene expression through binding miRNAs and RNA binding proteins. Genetic variation of circRNAs may affect complex traits/diseases by changing their binding efficiency to target miRNAs and proteins. There is a growing demand for investigations of the functions of genetic changes using large-scale experimental evidence. However, there is no online genetic resource for circRNA genes. Results: We performed extensive genetic annotation of 295,526 circRNAs integrated from circBase, circNet and circRNAdb. All pre-computed genetic variants were presented at our online resource, circVAR, with data browsing and search functionality. We explored the chromosome-based distribution of circRNAs and their associated variants. We found that, based on mapping to the 1000 Genomes and ClinVAR databases, chromosome 17 has a relatively large number of circRNAs and associated common and health-related genetic variants. Following the annotation of genome wide association studies (GWAS)-based circRNA variants, we found many non-coding variants within circRNAs, suggesting novel mechanisms for common diseases reported from GWAS studies. For cancer-based somatic variants, we found that chromosome 7 has many highly complex mutations that have been overlooked in previous research.Conclusion: We used the circVAR database to collect SNPs and small insertions and deletions (INDELs) in putative circRNA regions and to identify their potential phenotypic information. To provide a reusable resource for the circRNA research community, we have published all the pre-computed genetic data concerning circRNAs and associated genes together with data query and browsing functions at http://soft.bioinfo-minzhao.org/circvar.
Clinical Features and Surgical Outcomes of Subretinal Proliferation in Proliferative Diabetic Retinopathy
Authors: Wu Chan , Rongping Dai , Chen Youxin , Zhang Xiao , Chen Zhe 
Published: August 2020

Abstract
Background: To investigate the clinical characteristics, treatments and prognosis of subretinal proliferation (SRP) in patients with proliferative diabetic retinopathy (PDR).Methods: 154 patients (182 eyes) who received vitrectomy for PDR were retrospectively reviewed. Patients with SRP were enrolled as the study group, and those without SRP served as the control group. The following data were collected from their medical records: demographics, systemic and ophthalmologic findings, and treatment given specifically for SRP. The main outcome measures included the visual acuity, funduscopic examination and final anatomic success. The association between SRP and other systemic involvement of PDR was also analyzed. Results: There were 9 eyes (9 patients) in the study group and 145 eyes (145 patients) in the control group. The average fasting blood glucose was 11.48 ± 3.52 mmol/L and 8.72 ± 3.05 mmol/L, respectively (P = .048). The study group had a significantly higher proportion of tractional retinal detachment (TRD) (P < .0001) and a significantly lower proportion of vitreous hemorrhage (VH) (P = .0006). The rate of silicone oil usage was higher in the study group (P < .0001). No retinal break was found preoperatively or intraoperatively. Only one eye (11.1%) had undergone subretinal band removal procedure intraoperatively, and the final anatomical success rate was 100%. Conclusion: SRP in PDR was associated with higher blood sugar levels and TRD. The retina could reattach successfully after vitrectomy without removal or transection of the subretinal bands in most eyes.
Expression Patterns of Ciliopathy Genes ARL3 and CEP120 Reveal Roles in Multisystem Development
Authors: Powell L , Barroso-Gil M , Clowry Gavin J , Devlin Laura A , Molinari Elisa , Ramsbottom Simon A , Miles Colin G , Sayer John 
Published: August 2020

Abstract
Joubert syndrome and related disorders (JSRD) and Jeune syndrome are multisystem ciliopathy disorders with overlapping phenotypes. There are a growing number of genetic causes for these rare syndromes, including the recently described genes ARL3 and CEP120. We sought to explore the developmental expression patterns of ARL3 and CEP120 in humans to gain additional understanding of these genetic conditions. We used an RNA in situ detection technique called RNAscope to characterise ARL3 and CEP120 expression patterns in human embryos and foetuses in collaboration with the MRC-Wellcome Trust Human Developmental Biology Resource. This study provides insights into the potential pathogenesis of JSRD by uncovering the spatial expression of two JSRD-causative genes during normal human development.
Development and Validation of An Immune-Related Gene Pair Signature in Skin Cutaneous Melanoma
Authors: Xie Ran , Dong Suwei , Jiang Jie , Yang Conghui , Li Lanjiang , Zhao Sheng , Li Yunlei , Wang Chun , Li Shujuan , Xiao Yanbin , Chen Long 
Published: August 2020

Abstract
Skin cutaneous melanoma (SKCM) is a common skin malignancy worldwide, and its metastasis and mortality rates are high. The molecular characteristics exhibited by tumor-immune interactions have drawn the attention from researchers. Therefore, increased knowledge and new strategies to identify effective immune-related biomarkers may improve the clinical management of SKCM by providing more accurate prognostic information. In this study, we established a prognostic immune-related gene pair (IRGP) signature for predicting the survival of SKCM patients. The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases provided gene expression profiles together with clinical information, and the samples were randomly divided into three groups including the training, testing, and validation datasets. The regression model of least absolute shrinkage and selection operator (LASSO) helped to identify a 13-IRGP signature with a significant relation to the survival of SKCM patients. The training, TCGA, and independent sets have an average value of area under the curve of 0.79, 0.76, and 0.82, respectively. In addition, this 13-IRGP signature can noticeably divide SKCM patients into high-risk group and low-risk group with significantly different prognoses. Many biological activities such as gene family were enriched among the genes in our IRGP signature. While analyzing the risk signature and clinical characteristics, there was a large difference in the risk score between T stage and tumor stage grouping. Finally, we constructed a nomogram and forest plots of the risk score and clinical features. In summary, we developed a robust 13-IRGP prognostic signature in SKCM, which can identify and provide new insights into immunological biomarkers.
Anthropometry of the Coracoid Process: Surgical Considerations in Arthroscopic Coracoclavicular Ligament Reconstruction
Authors: Kuptniratsaikul Somsak , Prasertkul Natavut , Itthipanichpong Thun 
Published: August 2020

Abstract
Purposed: The purpose of this study was to measure the dimensions of the coracoid process from MRI imaging to find a safe area for drilling in arthroscopic coracoclavicular ligament reconstruction.Materials and methods: A retrospective study of patients who underwent MRI of the shoulder in our hospital between July 1, 2016 and June 31, 2018. Inclusion criteria included patients aged 20–60 years, BMI < 35 and height > 150 cm. Exclusion criteria included patients with a history of coracoid surgery, coracoid fracture or bone diseases affecting the coracoid bone. Measurement of the coracoid size was done in T1 MRI sagittal, coronal and axial views. Safe zone was defined as an area far from the medial and lateral base of the coracoid process, at least 5 mm each. Safe zone was then compared with the size of the drill hole to find an appropriately sized drill that would not violate the peripheral cortical coracoid bone.Results: Overall, 100 (male = 55, female = 45) patients were included in this study. Mean age, BMI and height of the patients were 48.5 years (range 22–58), 22.5 kg/m2 (range 18.5–28.4) and 164.5 cm (range 155–182), respectively. Mean safe area was 167.45 mm2 with males being slightly larger than females (male = 190.34 mm2, female = 140.99 mm2). The drill hole area was calculated by the radius (r) of the drill ( compared with the safe area. The maximum drill size for the men was 7 mm (154 mm2) and 6 mm (113.14 mm2) for women. However, we recommended a smaller drill size to account for any errors in position and technique.Conclusion: The safe area at the coracoid base was slightly larger in males as compared to females and the proper drill bit size was suggested to be less than 7 mm in males and 6 mm in females.
Cultural Competence of Australian Dental Students
Authors: Mariño Rodrigo , Satur Julie , Tuncer Eren , Tran Megan , Milford Elizabeth , Tran Vivien , Tran Phuong , Tsai Richard 
Published: August 2020

Abstract
Background: Australia possesses a highly multicultural demographic, and thus dental practitioners are likely to encounter culturally and linguistically diverse individuals regularly. It is important for dental practitioners to be culturally competent, however, cultural competency education is highly variable in the curricula of dentistry and oral health courses in Australia, and research is largely limited to dentistry students. This study aims to investigate and compare perceived attitudes, beliefs and practices of cultural competence amongst first and final year Doctor of Dental Surgery (DDS) and Bachelor of Oral Health (BOH) students at the University of Melbourne Dental School.Methods: Following ethics approval, anonymous questionnaires were completed by 213 participants. The questionnaire was adapted from Schwarz’s Healthcare Provider Cultural Competence Instrument (HPCCI) and consisted of five scales. Data was analysed using SPSS V 24.0 software.Results: A total of 213 students participated in this study (response rate = 88%) The majority of participants were female (n=114, 53.5%) and the mean age of 23.5 years (range 18 - 40). The majority of participants were Australian born (n=110) with 74.6 percent (n=159) first generation Australians. Participants who identified as Australian represented 35.7 percent (n=76) with 66.1 percent (n=141) identified as partly Australian. Multivariate analysis indicated that, after controlling for other independent variables in the model, those who had the highest cultural competence score were female, who self-identify as “Australian”, who were in the final year. Furthermore, those who were in the final BOH year scored significatively higher than final year DDS students.Conclusion: The findings of this study suggest that there is a significant difference in students self-reported cultural competence at different stages of their education. This may be attributed to differences in cultural competence education, scope of practice and the type of patient encounters that students may experience. Future research should involve follow up to create longitudinal data, as well as research at other dental schools in Australia and overseas.
Time trends in hospital admissions in very elderly patients (≥85 years-old) in Spain: data from the Spanish National Discharge Database (2000 - 2015)
Authors: Fernández Sergio Palacios , Salcedo Mario , Gonzalez-Alcaide Gregorio , Ramos-Rincon Jose-Manuel 
Published: August 2020

Abstract
BackgroundThe aging population is an increasing concern in Western hospital systems. The aim of this study was to describe the main characteristics and hospitalization patterns in very elderly inpatients (≥ 85 years) in Spain from 2000 to 2015.MethodsRetrospective observational study analyzing data from the minimum basic data set, an administrative registry recording each hospital discharge in Spain since 1997. We collected administrative, economic and clinical data for all discharges between 2000 and 2015 in patients aged 85 years and older, reporting results in three age groups and four time periods to assess differences and compare trends.ResultsThere were 4,387,326 admissions in very elderly patients in Spain from 2000 to 2015, representing 5.32% of total admissions in 2000–2003 and 10.42% in 2012–2015. The pace of growth was faster in older age groups, with an annual percentage increase of 6% in patients aged 85–89 years, 7.79% in those aged 90–94 years, and 8.06% in those aged 95 and older. The proportion of men also rose (37.3–39.7%, p < 0.001), and they had a higher incidence of hospitalization than women (385 discharges/1000 men versus 280 discharges/1000 women in 2012–2015).There were 4,387,326 admissions in very elderly patients in Spain from 2000 to 2015, representing 5.32% of total admissions in 2000–2003 and 10.42% in 2012–2015. The pace of growth was faster in older age groups, with an annual percentage increase of 6% in patients aged 85–89 years, 7.79% in those aged 90–94 years, and 8.06% in those aged 95 and older. The proportion of men also rose (37.3% to 39.7%, p<0.001), and they had a higher incidence of hospitalization than women (385 discharges/1000 men versus 280 discharges/1000 women in 2012–2015).Mortality decreased from 14.64% in 2000–2003 to 13.83% in 2012–2015 (p<0.001), and mean length of stay from 9.98 days in 2000–2003 to 8.34 days in 2012–2015. Costs per hospital stay increased from 2000 to 2011, from EUR 4611 in 2000–2003 to EUR 5212 in 2008–2011, before dropping to EUR 4824 in 2012–2015. The 10 most frequent discharge diagnoses in the period 2000-2003 were: femoral neck fracture (8.07%), heart failure (7.84%), neoplasms (7.65%), ischemic encephalopathy (6.97%), pneumonia (6.36%), chronic obstructive pulmonary disease (4.23%), ischemic cardiomyopathy (4.2%), other respiratory diseases (3.87%), other alterations of urethra and the urinary tract (3.08%), and cholelithiasis (3.07%). ConclusionsThe very elderly population is growing in Spanish hospitals, and within this group, patients are getting older and more frequently male. The mean length of stay, cost of stay, and mortality are decreasing. Decompensation of chronic diseases, neoplasms and infections are the most common causes of admission.
Effects of vibration on chronic leg edema in chair-bound older adults: A randomized pilot trial
Authors: Tsuchiya Sayumi , Sato Aya , Ueda Terumi , Dai Misako , Okuwa Mayumi , Nakatani Toshio , Sugama Junko 
Published: August 2020

Abstract
BackgroundElderly individuals can easily develop leg edema that can become chronic, which may result in various problems. Therefore, appropriate care for the edema should be provided. In some cases, chronic leg edema among elderly individuals cannot be controlled by the standard care such as leg elevation or compression. A previous study reported that vibration benefited upper limb lymphedema; however, its effects on chronic leg edema are not yet clarified. Therefore, this study aimed to clarify the effects of vibration for reducing chronic leg edema among chair-bound elderly individuals.MethodsFor participant allocation, a computer-generated list of random numbers was used. Nursing home residents aged ≥65 years with chronic leg edema who spent more time sitting than standing or lying during the day were randomly assigned to the intervention (n = 7) or control group (n = 7). The intervention group underwent vibration therapy three times a day for 2 weeks at 47 Hz and 1.78 m/s2 frequency and horizontal vibration acceleration, respectively. The pitting test was performed at 22 sites, and participants’ pitting scores were calculated based on the pitting depth. Pitting score changes at pre- and post-intervention were compared between the intervention and control groups. Both participants and investigators were not blinded to group assignment.ResultsThe median age of the intervention and control groups was 86 and 84 years, respectively. Participants’ characteristics and edema severity at baseline were not significantly different. The median total pitting score change in the intervention group was −0.4 (interquartile range: −5.3–1.8), which was significantly lower than that of the control group (2.0 [interquartile range: 1.0－5.3], P = 0.01). The intervention group was more likely to have controlled edema (64.3%) than the control group (21.4%) (χ2 (1) = 5.25, P = 0.02).ConclusionsThe intervention group was more likely to have controlled edema than the control group, suggesting that vibration could prevent the worsening of chronic leg edema in chair-bound elderly individuals.Trial registration: UMIN Clinical Trials Registry, UMIN000017716. Registered 1 July 2015, https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000020522
Morphology, Ontogeny and Molecular Phylogeny of a New Urostylid Ciliate, Bakuella (Pseudobakuella) Guangdongica n. sp. (Protista, Ciliophora, Hypotrichia) from Southern China
Authors: Wang Jingyi , Li Jingbao , Wang Yurui , Al-Farraj Saleh A. , Shao Chen 
Published: August 2020

Abstract
Background: Spirotrich ciliates are one of the most diverse groupsin the phylum Ciliophora and are widely distributed in marine, freshwater, and terrestrial biotopes. Many nominal species are, however, poorly known and their systematic positions remain uncertain due to the lack of information concerning their infraciliature, morphogenesis, and gene sequences. In this paper, the morphology and morphogenesis of Bakuella (Pseudobakuella) guangdongica n. sp. were studied, in addition, genomic DNA was extracted in order to sequence the small subunit rDNA. Results: Bakuella (Pseudobakuella) guangdongica n. sp. is characterized by 150–225 µm in vivo; 35–42 adoral membranelles; three to five buccal, two frontoterminal and eight to 13 transverse + pretransverse cirri; midventral complex comprised of 10–20 midventral pairs and two midventral rows extending to transverse cirri; posterior part of marginal rows slightly overlapped; colorless cortical granules about 1 μm across, arranged in small groups; soil habitat. Its main ontogenetic features are: (1) in the proter, the parental adoral zone of membranelles is completely renewed by new structures; (2) in the opisthe, the oral primordium originates apokinetally, some old midventral cirri join the formation of frontoventral-transverse cirral anlagen; (3) the anlagen for marginal rows and dorsal kineties develop intrakinetally; and (4) the numerous macronuclear nodules fuse into a single mass before dividing. Phylogenetic analyses based on the SSU rDNA sequence suggests the non-monophyly of the genus Bakuella. Conclusions: The morphology, morphogenesis and molecular phylogeny of the new hypotrichous ciliate, Bakuella (Pseudobakuella) guangdongica n. sp. were studied, the phylogenetic analyses show that the Bakuella is polyphyletic in the SSU rDNA.