bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sunlight Exposed Body Surface Area&nbsp;is Associated With Serum 25-hydroxyvitamin D (25(OH)D) Level&nbsp;in Pregnant Minangkabau Women,&nbsp;Indonesia
Authors: Ilmiawati Cimi , Oviana Athica , Friadi Andi 
Published: October 2019

Abstract
Background: Vitamin D deficiency is highly prevalent in women, while living in a tropical country with year-round abundance of sunlight as the main source of vitamin D does not seem to guarantee adequate serum 25(OH)D. While living in the tropics, Minangkabau women are known to dress specifically according to their culture. This study was aimed to elucidate the association of sunlight exposed body surface area with serum 25(OH)D in pregnant Minangkabau women of Indonesia. Methods: A cross-sectional study was carried out on 88 Minangkabau women in late pregnancy. Data were collected by using questionnaire and 24-hour food recall, skin pigmentation was determined by Fitzpatrick scale, body surface area exposed to sunlight was assessed and serum 25(OH)D was quantified by ELISA method. Results: Nearly half of the subjects (n=40; 45.5%) were deficient in vitamin D (<20 ng/ml) with serum 25(OH)D level23.0±10.0 ng/ml (mean±SD) and estimated daily intake of vitamin D 5.6±3.9 µg/1000 kcal/day (mean±SD). Median percentage of body area exposed to sunlight was 15.8%. There were no differences in serum 25(OH)D levels according to sunlight exposure time, skin pigmentation type, and sunscreen use. Percentage of body area exposed to sunlight was positively correlated with serum 25(OH)D level (Spearman’s ρ =0.403; p<0.001). Conclusions: Vitamin D deficiency is prevalent in pregnant Minangkabau women and they need to consider increasing their body area exposure to sunlight. Keywords: body surface area, pregnant, sunlight exposure, vitamin D
Age-specific risk factors for adverse maternal and neonatal outcomes in Xiamen, China: A population-based retrospective study
Authors: Chen Yin-ling , Han Li-li , Su Wei-juan , Lv Fu-ping , Chen Zheng , Huang Bing-kun , Wang Li-ying , Song Hai-qu , Li Xuejun 
Published: October 2019

Abstract
Background: Elderly mothers are increasingly in China. We are aims to explore whether association between pregnancy to delivery interval and adverse pregnancy outcomes is affected by maternal age. Methods: A population-based retrospective study was performed in Xiamen, China. Data were derived from the Medical Birth Registry of Xiamen from 2011 to 2018. Multivariable logistic regression was used to conduct multivariable analyses based on adjusting the factors to evaluate the effect of maternal age on pregnancy outcomes. Results: Among 77,859 pregnant women with specific age, gestational diabetes mellitus (GDM) risk were increased for women aged 40 years or older (42.9%; aRR, 3.84 (3.26-4.51); P < 0.001), but not for pregnant women aged less than 25 years (9.2%; aRR, 0.60 (0.54-0.68)). Increased cesarean, preterm birth, large-for-gestational age (LGA), and low birth weight risks were more pronounced for pregnant women aged 40 years or older (66.5%, 8.4%, 26.5%, and 6.7%, respectively; aRR, 3.77 (3.14-4.52), 1.26 (0.90-1.79), 1.31 (1.08-1.60), and 1.10 (0.74-1.65), respectively; all P < 0.001). Risk of Apgar < 7 at 5 minutes were increased for women < 25 years old compared with women 35 to 39 years old (0.2% vs. 0.1%; aRR, 2.05 (0.85-4.93) vs. 0.63 (0.14-2.86)). Conclusion: Advanced maternal age increased risk of adverse pregnancy outcomes. The risk ratio of GDM, cesarean, preterm birth, LGA, and low birth weight is higher, which suggests pregnant women should guard against related risk factors and choose an appropriate mode of production. Furthermore, pregnant women should choose an ideal age for pregnancy to make themselves and child healthy.
Characterization and clinical evaluation of microsatellite instability and loss of heterozygosity within tumor-related genes in colorectal cancer
Authors: Huo Xueyun , Feng Dandan , Zhang Shuangyue , Li Zhenkun , Li Xiaohong , Li Changlong , Guo Meng , Wang Jin , Zhang Zhongtao , Lu Qingxian , Du Xiaoyan , Bai Zhigang , Chen Zhenwen 
Published: October 2019

Abstract
Background: Microsatellite instability (MSI) has been known as a biomarker for better outcome in colorectal cancer (CRC). However, the conclusion is controversy. In addition, MS can also be a useful marker for loss of heterozygosity (LOH) of genes but it has not been well studied yet. Here, aimed to clarify the predictive value of MSI/LOH within tumor-related genes in CRCs, we detected MSI/LOH of MSs in tumor-related genes and the Bethesda (B5) panel and further analyzed the relationship between MSI/LOH and clinical features or outcomes. Results: As expected, the MSI rate of B5 loci were all very high, suggesting that B5 panel criterion is powerful for MSI status determining of CRCs. Interestingly, MSI/LOH of 2 loci in the B5 panel and 12 loci in tumor-related genes were associated with poorer outcome while MSI/LOH of B5 panel was failed to predict outcomes of CRCs. MSI of BAT25, MSI/LOH of BAT26 and MSI of B5 panel showed closer relationship with mucinous carcinoma. In addition, LOH-H of B5 panel associated with more lymphatic metastasis. Conclusions: In summary, MSI/LOH of certain loci or whole panel of B5 were related to the clinical features, and several loci within tumor-related genes showed a prognostic value in outcomes of CRCs.
Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension
Authors: Luo Jing , Liu Zhenwei , Li Chenlu , Wang Ruochen , Fang Jinxia , Lu Saisai , Guo Jing , Zhu Xiaochun , Wang Xiaobing 
Published: October 2019

Abstract
Background: Pulmonary arterial hypertension (PAH) is a life-threatening condition that gets worse over time. Despite advances in the development of strategies for treating PAH, prognosis of the disease remains unsatisfactory, especially for advanced PAH. The aim of this study was to explore potential crucial genes and pathways associated with PAH that can be used as potential biomarkers for early diagnosis.Results: Gene expression profile of pulmonary tissues from 27 PAH patients and 22 normal controls were downloaded from public database (GSE53408 and GSE113439). A total of 521 differentially expressed genes (DEGs), including 432 up-regulated DEGs and 89 down-regulated DEGs were identified using “limma” package in R. Functional enrichment analysis showed that these DEGs were mainly enriched in mitotic cell cycle process, mitotic cell cycle and microtubule cytoskeleton organization. Moreover, five key genes (CDK1, SMC2, SMC4, KIF23, and CENPE) were identified based on the comprehensive evaluation of PPI analysis, modular analysis and cytohubba’s analysis, then further validated in another transcriptomic data set associated with PAH from public database (GSE33463). Furthermore, these hub genes were mainly enriched in promoting mitotic cell cycle process, which may be closely associated with the pathogenesis of PAH. We also found that the predicted miRNAs targeting these hub genes were found to be enriched in TGF-β and Hippo signaling pathway.Conclusion: These findings are expected to gain a further insight into the development of PAH and provide a promising index for the detection of PAH.
Multistability in the epithelial-mesenchymal transition network
Authors: Xin Ying , Cummins Breshine , Gedeon Tomas 
Published: October 2019

Abstract
Background: The transitions between epithelial (E) and mesenchymal (M) cellphenotypes are essential in many biological processes like tissue development andcancer metastasis. Previous studies, both modeling and experimental, suggestedthat in addition to E and M states, the network responsible for these phenotypesexhibits intermediate phenotypes between E and M states. The number andimportance of such states is subject to intense discussion in the EMT community.Results: Previous modeling eorts used traditional bifurcation analysis to explorethe number of the steady states that correspond to E, M and intermediate statesby varying one or two parameters at a time. Since the system has dozens ofparameters that are largely unknown, it remains a challenging problem to fullydescribe the potential set of states and their relationship across all parameters.We use the computational tool DSGRN (Dynamic Signatures Generated byRegulatory Networks) to explore the intermediate states of an EMT modelnetwork by computing summaries of the dynamics across all of parameter space.We nd that the only attractors in the system are equilibria, that E and M statesdominate across parameter space, but that bistability and multistability arecommon. Even at extreme levels of some of the known inducers of the transition,there is a certain proportion of the parameter space at which an E or an M stateco-exists with other stable steady states.Conclusions: Our results suggests that the multistability is broadly present inEMT network across parameters and thus response of cells to signals maystrongly depend on their history and genetic background.Keywords: Epithelial-Mesenchymal transition; multistability; network models
The pattern of life events preceding suicide among the elderly in rural China: a national case-control psychological autopsy study
Authors: Mo Qiqing , Wang Guojun , Jia Cunxian , Niu Lu , Zhou Liang , Ma Zhenyu 
Published: October 2019

Abstract
Background Life events associated with an increased risk of suicide and prior to the suicide in China different from those in Western countries. But there is a lack of summary of the elderly life event patterns on Chinese suicide. The aim of this study was to identify the pattern of life events which precede suicide among the elderly in a Chinese culture context.Methods A two-stage stratified cluster sampling method was used in order to select research sites and 12 counties were randomly selected to recruit cases within three provinces. A case-control psychological autopsy study was then adopted. The study population consisted of 242 suicide cases each with an age of 60 and above, and 242 age-, sex-, and location-matched community living controls. During face-to-face interviews, the information from subjects and proxy informants was obtained by standardized trained investigators. Life events were measured using a 46-item Life Event Scale for the Elderly (LESE).Results About 99.6% of suicides and 88.4% of controls resulted from the experience of at least one life event. The elderly who had committed suicide experienced more long-term life events. The top three most frequent life events were being diagnosed with chronic disease, hospitalization, and being diagnosed with terminal illness. Women more often experienced the death of spouse, while men experienced more hospitalization, and were more likely to be diagnosed with terminal illness and suffer family poverty. The risk of suicide among the elderly who experience life events was 12.8 times higher than that in people who did not experience life events. The elderly who were having unstable marital status, suffering from physical diseases and diagnosed with mental disorders, are more likely to commit suicide.Conclusions Understanding the pattern of life events which precede suicides among the elderly in rural China plays an important role in the prevention of suicide. Several different long-term life events can lead to suicidal behavior, and the effect of life events on suicide is cumulative. Being diagnosed with a serious disease may be one of the risk signals for suicide among the elderly.
Impact of GTF2H1 and RAD54L2 gene polymorphisms on the non-small cell lung cancer with Platinum chemotherapy in high altitude areas
Authors: Li Miao , Chen Rong , Ji Baoyan , Fan Chunmei , Wang Guanying , Yue Chenli , Jin Guoquan 
Published: October 2019

Abstract
Background Repair pathway genes are closely related to the sensitivity of tumor patients to drugs. It is particularly important to study the correlation between non-small cell lung cancer (NSCLC) and DNA repair gene ( GTF2H1 and RAD54L2 ) in high altitude areas.Materials and methods Four SNPs on RAD54L2 and five SNPs on GTF2H1 were genotyped via the Agena MassARRAY in 506 age-and gender-matched patients with NSCLC and 510 healthy controls. The influence of GTF2H1 and RAD54L2 on lung cancer was assessed by logistic regression analysis. The effects of polymorphisms of these two genes on the efficacy and side effects of NSCLC patients with platinum-based chemotherapy were further evaluated.Results RAD54L2 rs9864693 GC heterozygote genotype was increased the risk of NSCLC (OR = 1.33, 95%CI: 1.01 – 1.77, p = 0.045). Stratified analysis found that GTF2H1 rs4150667 promoted the risk of lung cancer in individuals younger than 59 years group (OR = 1.32, 95%CI: 1.00 – 1.75, p = 0.048). However, the individuals with GTF2H1 rs3802967 CT-TT reduced the risk of the lung squamous cell carcinoma by 32% (OR = 0.68, 95%CI: 0.46 – 0.99, p = 0.045). The patients with GTF2H1 rs3802967 TT genotypes showed a significantly higher curative effect (OR = 2.65, 95%CI: 1.14 - 6.15, p = 0.023); patients with GTF2H1 rs3802967 TT genotypes showed a significantly lower risk of side effects (OR = 0.32, 95%CI: 0.10 - 0.98, p = 0.047).Conclusion Our study indicated that RAD54L2 and GTF2H1 gene was associated with NSCLC susceptibility.
A meta-analysis of the endothelial protein C receptor rs867186 genotype in malaria
Authors: Yang Lin , Xin Ruilian , Guo Shanchun , Liu Mingli 
Published: October 2019

Abstract
BACKGROUND : During P. falciparum infection, the binding of P. falciparum erythrocyte membrane protein 1 (PfEMP1) to endothelial cells (EC) results in the sequestration of pRBC. Several receptors located on the endothelial cells, including intercellular adhesion molecule 1 (ICAM-1), CD36, and endothelial protein C receptor (EPCR), contribute to PfEMP1 adhesion to the microvasculature. PfEMP1, expressed on the surface of parasitized red blood cells (pRBC), is composed of cysteine-rich interdomain regions (CIDR) and Duffy binding-like (DBL) domains. CIDRα1 competitively binds to EPCR with activated protein C (APC) and impairs cytoprotective and anticoagulant effects by APC, which plays important roles in severe malaria (SM) pathogenesis such as cerebral malaria (CM) and severe malaria anemia (SMA). The strategy to inhibit EPCR binding to pRBC while concomitantly strengthen its binding to APC may be crucial in restoring disrupted protein C (PC) system’s function. The purpose of this study is to evaluate the association between malaria severity and the EPCR genotypes as well as with soluble EPCR (sEPCR), and the study also addresses the physiological relevance of EPCR genetic polymorphism.
RESULTS : In this study, we conducted a meta-analysis on the eligible studies by comparing the frequency of EPCR rs867186-GG versus rs867186-GA and -AA genotype in SM, mild malaria (MM) or uncomplicated malaria (UM) patients and healthy individuals from Thailand, Uganda, Benin, Tanzania, and Ghana. We also determined the relationship between rs867186 genotype and sEPCR levels. Our results showed that the genotype rs867186-GG is higher in MM/UM than in SM patients. SM patients carrying the rs867186-GG genotype have higher plasma soluble EPCR (sEPCR) levels than in rs867186-AG and rs867186-AA carriers. MM/UM patients carrying the rs867186-AG genotype have significantly higher level of sEPCR compared to those carrying rs867186-AA. Similarly, the rs867186-GG is associated with high sEPCR level in healthy individuals.
CONCLUSIONS : This meta-analysis demonstrates that pRBCs and EPCR interactions are associated with malaria severity, and treatments that block their binding via PfEMP1 CIDRα1 could be a potential therapy for SM.
Ekoteologi Fungsi Hutan Oenaek: Penyimpangan Paradigma Ekologis Menuju Perilaku Eksploitatif
Authors: Awang Nirwasui Arsita , Setyawan Yusak B , Timo Ebenhaizer L Nuban 
Published: October 2019

This study aims at describing and analyzing the change of Camplong residents’ perspective towards the function of forest which brings an impact on the damage of Oenaek Forest. In addition, the purpose of this study is also to conduct an ecotheological observation about this viewpoint shift. There is a Natural Tourism Park in Camplong with various kinds of flora and fauna. In Timorese belief, this forest functions as a palace for Usi (the highest god) and a shelter for the spirits of passed away ancestors. The shift of the forest status fromnatural forest (restricted forest) to productive forest has caused the change of residents’ perspective towards the function of the forest. In the past they used to perceive the forest as a subject, but now it has been seen as an object of economic enterprise. The change of this standpoint has influenced the behavior of Camplong people who live around the forest area: the forest is no longer served as a place for celebrating traditional rituals, but merely as a tourist destination. The method applied in this research is qualitative descriptive whichis carried out through observation and in-depth interview.
Design of vibration standard device based on random comparison method
Authors: Gao Tao , Lu Chen , Chen Pu , Li Meng , Han Mei 
Published: October 2019

In order to meet the needs for aircraft vibration transducer and measurement equipment to be calibrated at the job site, it is necessary to design a compact, dynamic and functional vibration standard device. This paper introduces a vibration standard device based on random comparison method. The structure and working principle of software and hardware are described in detail. Unlike the calibration requirements under laboratory conditions, on-site calibration requires that the calibration process be more consistent with the actual operating conditions. A random vibration comparison calibration method using random signals as excitation sources is designed. The vibration standard device can be maneuvered from the laboratory environment to the aircraft maintenance site. It has been proved measurement uncertainty of the system is 1.0 % (k= 2) by the method of control accuracy.
Accuracy evaluation of GPM multi-satellite precipitation products in the hydrological application over alpine and gorge regions with sparse rain gauge network
Authors: Chen Jiachao , Wang Zhaoli , Wu Xushu , Chen Xiaohong , Lai Chengguang , Zeng Zhaoyang , Li Jun 
Published: October 2019

Abstract
With the release of Global Precipitation Measurement Integrated Multi-satellitE Retrievals for GPM products, hydrologists can obtain precipitation data with higher resolution and wider coverage. However, great uncertainties still exist in the accuracy and hydrological utility of these data in alpine and gorge regions with sparse gauge stations. In this study, the Lancang River Basin in China was used as an example, and near real-time products (IMERG-E and IMERG-L) and post-processed products (IMERG-F and TMPA 3B42-V7) were evaluated. Different indexes and methods were applied to evaluate the accuracy of these products. The variable infiltration capacity hydrological model was adopted to evaluate their hydrological utility. The following findings were obtained. (1) Compared with observed precipitation data, the near real-time products tend to underestimate, while the post-processed products tend to overestimate precipitation. The performance of the four products in winter is poor. (2) IMERG products offer improvements in two aspects: first, the near real-time products achieve good accuracy and second, the detectability and the accuracy in gorge areas have been greatly improved. (3) The near real-time products have the potential for hydrological applications. The best simulation result was obtained based on IMERG-F, followed by 3B42-V7, IMERG-E, and IMERG-L. (4) The four products can provide reliable precipitation data for the hydrological application over the Lancang River Basin.
Switching from Conventional to Nano-natural Phytochemicals to Prevent and Treat Cancers: Special Emphasis on Resveratrol
Authors: Salman Ul Islam  , Ahmed Muhammad B. , Mazhar Ul-Islam  , Shehzad Adeeb , Lee Young S. 
Published: October 2019

Background:
Natural phytochemicals and their derivatives have been used in medicine since prehistoric
times. Natural phytochemicals have potential uses against various disorders, including cancers. However,
due to low bioavailability, their success in clinical trials has not been reproduced. Nanotechnology has played a
vital role in providing new directions for diagnosis, prevention, and treatment of different disorders, and of cancer
in particular. Nanotechnology has demonstrated the capability to deliver conventional natural products with poor
solubility or a short half-life to target specific sites in the body and regulate the release of drugs. Among the natural
products, the phytoalexin resveratrol has demonstrated therapeutic effects, including antioxidant, antiinflammatory,
and anti-proliferative effects, as well as the potential to inhibit the initiation and promotion of
cancer. However, low water solubility and extensive first-pass metabolism lead to poor bioavailability of resveratrol,
hindering its potential. Conventional dosage forms of resveratrol, such as tablets, capsules, dry powder, and
injections, have met with limited success. Nanoformulations are now being investigated to improve the pharmacokinetic
characteristics, as well as to enhance the bioavailability and targetability of resveratrol.

Objectives:
This review details the therapeutic effectiveness, mode of action, and pharmacokinetic limitations of
resveratrol, as well as discusses the successes and challenges of resveratrol nanoformulations. Modern nanotechnology
techniques to enhance the encapsulation of resveratrol within nanoparticles and thereby enhance its therapeutic
effects are emphasized.

Conclusion:
To date, no resveratrol-based nanosystems are in clinical use, and this review would provide a new
direction for further investigations on innovative nanodevices that could consolidate the anticancer potential of
resveratrol.
Nano-enhanced Optical Gene Delivery to Retinal Degenerated Mice
Authors: Batabyal Subrata , Gajjeraman Sivakumar , Bhattacharya Sulagna , Wright Weldon , Mohanty Samarendra 
Published: October 2019

Background:
The efficient and targeted delivery of genes and other impermeable therapeutic
molecules into retinal cells is of immense importance for the therapy of various visual disorders.
Traditional methods for gene delivery require viral transfection, or chemical methods that suffer from
one or many drawbacks, such as low efficiency, lack of spatially targeted delivery, and can generally
have deleterious effects, such as unexpected inflammatory responses and immunological reactions.

Methods:
We aim to develop a continuous wave near-infrared laser-based Nano-enhanced Optical Delivery
(NOD) method for spatially controlled delivery of ambient-light-activatable Muti-Characteristic
opsin-encoding genes into retina in-vivo and ex-vivo. In this method, the optical field enhancement by
gold nanorods is utilized to transiently permeabilize cell membrane, enabling delivery of exogenous
impermeable molecules to nanorod-binding cells in laser-irradiated regions.

Results and Discussion:
With viral or other non-viral (e.g. electroporation, lipofection) methods, gene
is delivered everywhere, causing uncontrolled expression over the whole retina. This will cause complications
in the functioning of non-degenerated areas of the retina. In the NOD method, the contrast
in temperature rise in laser-irradiated nanorod-attached cells at nano-hotspots is significant enough to
allow site-specific delivery of large genes. The in-vitro and in-vivo results using NOD, clearly demonstrate
in-vivo gene delivery and functional cellular expression in targeted retinal regions without compromising
the structural integrity of the eye or causing immune response.

Conclusion:
The successful delivery and expression of MCO in the targeted retina after in-vivo NOD
in the mice models of retinal degeneration opens a new vista for re-photosensitizing retina with geographic
atrophies, such as in dry age-related macular degeneration.