bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inclusion of fluted pumpkin (Telfaria occidentalis) fortified bread waste in broiler chickens’ diets
Authors: Adegbenro Muyiwa , Ayeni Oluwafemi A. , Agbede Johnson O. , Aletor Valentine A. 
Published: June 2020

Abstract
Background
The objective of this study was to produce a least-cost ration from the use of fluted pumpkin leaf meal and bread waste in broiler chickens’ diets. Fluted pumpkin leaves (FPL) were sourced in Akure and its environs. The leaves were air-dried and milled to produce fluted pumpkin leaf meal (FPLM), while bread wastes were collected from different bakeries in Ilara-mokin and Akure towns, Ondo State, Nigeria. The bread wastes were sun-dried and milled and tagged BWM. The fluted pumpkin meal and bread waste meal were mixed in ratio 1:9 (1 kg FPLM + 9 kg BWM) to produce the fortified bread waste meal (FBWM) and used as one of the feed ingredients in broiler chickens’ diets. The FBWM were included in the diets at 0, 5, 10 and 15% to serve as diets I, II, III and IV, respectively. Two hundred day-old Abor-acre broiler chicks were randomly allotted to the four (4) dietary treatments at fifty (50) chicks per treatment at five (5) replicates per treatment in a completely randomized design. The experimental diets (starter and finisher) and freshwater were offered to the chicks ad libitum for fifty-six (56) days.

Results
The results showed that the final weights and total weight gain were significantly (P < 0.05) influenced by dietary treatments. The highest final weight (2.12 ± 13.16 kg/bird) and the highest total weight gain (2.08 ± 13.14 kg/bird) were recorded in birds fed diet I, while the lowest final weight (1.73 ± 11.67 kg/bird) and the lowest total weight gain (1.69 ± 11.15 kg/bird) were recorded in birds fed diet IV. There was no significant (P > 0.05) difference in the feed conversion ratio for all birds fed diets I–IV. The highest dressed weight (92.64 ± 8.30%) and the highest eviscerated weight (79.52 ± 6.50%) were recorded in birds fed diet I. The lowest cholesterol (21.23 ± 3.15 mg/dl) was recorded in bird fed diet IV and the highest (32.78 ± 3.78 mg/dl) in birds fed diet I. Highest net profit (N 683.27) was recorded in bird fed diet IV.

Conclusion
Conceivably, within the limit of this study, the inclusion of FBWM in the diet of broiler chickens at 0% had better results than other test diets, but looking at the level of significant, up to 5% is practicable considering the final weight and weight gain. Bird fed 15% yielded the highest profit/bird produced.
Exploring the organic–inorganic interface in biosilica: atomistic modeling of polyamine and silica precursors aggregation behavior
Authors: Eckert Hagen , Montagna Maria , Dianat Arezoo , Gutierrez Rafael , Bobeth Manfred , Cuniberti Gianaurelio 
Published: June 2020

AbstractDiatoms are a significant group of algae displaying a sizeable morphological diversity, whose underlying structure arises from nanopatterned silica. Extensive experimental evidence suggests that a delicate interplay between various organic components and polysilicic acid plays a crucial role in biosilica mineralization. Thus, gaining insight into the properties of this organic–inorganic interface is of great interest in understanding the mechanisms controlling biosilica formation over different length scales. In this work, we use all-atom Molecular Dynamics simulations to investigate the aggregation behavior of polysilicic acid and silica nanoparticles in solution in the presence of protonated long-chain polyamines with a focus on the nature of the driving forces mediating the organic–inorganic aggregation process. Our results show that electrostatic forces between organic and inorganic species are the dominant interaction responsible for largely preserving the structural integrity of the organic–inorganic aggregates in solution. Thus, aggregates involving electrically neutral polysilicic acid are fully dissolved in an aqueous environment, since hydrogen bonding and van der Waals interactions turn out to be not strong enough to keep the aggregates together. Our main simulation results are in qualitative agreement with in vitro experiments, so that we expect they can contribute to shedding light on the initial stages of biosilica mineralization in diatoms.
Primary cardiac tumors: a spectrum of pathologies and scenarios
Authors: Sanad Mohammed , Arafa Sherif , Hegazy Mohammed Adel , Abdel Hamid Wael Abdel Aziz 
Published: June 2020

Abstract
Background
Cardiac tumors are rare, and their diagnosis is challenging. The level of evidence for the management of cardiac tumors came from case reports or small case series. We aim to present our multicenter experience in the diagnosis and management of cardiac tumors.

Results
We performed a prospective study, including 82 cases of cardiac tumors encountered at three centers in 5 years. The mean age was 62 ± 5.8 years old (range 2 months–74 years), and 91.4% were females constituted. The median EuroSCORE II was 4.27%. All cases underwent radical excision of the tumor. Five cases underwent additional mitral valve replacement and one case underwent coronary bypass grafting. Pathological diagnoses were left atrial myxomas (n = 65, 79.3%), right atrial myxomas (n = 5, 6.1%), bi-atrial myxomas (n = 2, 2.4%), right ventricular outflow tract myxoma (n = 1, 1.2%), myxosarcoma (n = 1, 1.2%), thymoma (n = 1, 1.2%), recurrent hemangioendothelioma (n = 1, 1.2%), round cell tumor (n = 1, 1.2%), cardiac lymphoma (n = 1, 1.2%), rhabdomyomas (n = 2, 2.4%), and renal cell carcinomas (n = 2, 2.4%). Two patients (2.4%) had re-exploration for bleeding. One patient (1.2%) with right atrial thymoma experienced a severe vasoplegia with unstable hemodynamics. Two patients had operative mortality (2.4%), one with recurrent cardiac hemangioendothelioma. During 1-year follow-up, one patient (1.2%) died because of metastatic myosarcoma. Recurrence was reported in 2 patients (2.4%).

Conclusions
The clinical scenario of cardiac tumors depends mainly on tumor location and size rather than the histopathology. Surgical resection is feasible in benign tumors while very challenging in malignant tumors.
Vulvar Squamous Cell Carcinoma: Comprehensive Genomic Profiling of HPV+ Versus HPV– Forms Reveals Distinct Sets of Potentially Actionable Molecular Targets
Authors: Williams Erik A. , Werth Adrienne J. , Sharaf Radwa , Montesion Meagan , Sokol Ethan S. , Pavlick Dean C. , McLaughlin-Drubin Molly , Erlich Rachel , Toma Helen , Williams Kevin Jon , Venstrom Jeff M. , Alexander Brian M. , Shah Nikunj , Danziger Natalie , Hemmerich Amanda C. , Severson Eric A. , Killian Jonathan Keith , Lin Douglas I. , Ross Jeffrey S. , Tse Julie Y. , Ramkissoon Shakti H. , Mochel Mark C. , Elvin Julia A. 
Published: June 2020

PURPOSE Vulvar squamous cell carcinoma (vSCC) encompasses two predominant variants: one associated with detectable high-risk strains of human papillomavirus (hrHPV) and a second form often occurring in the context of chronic dermatitis in postmenopausal women. Genomic assessment of a large-scale cohort of patients with aggressive vSCC may identify distinct mutational signatures. MATERIALS AND METHODS Tumor samples from a total of 280 patients with vSCC underwent hybridization capture with analysis of up to 406 cancer-related genes. Human papillomavirus (HPV) sequences were detected by de novo assembly of nonhuman sequencing reads and aligned to the RefSeq database. Immunohistochemistry for programmed death-ligand 1 (PD-L1) was assessed. RESULTS One hundred two of 280 vSCCs (36%) contained hrHPV sequences, predominantly HPV 16 (88%). The HPV-positive (HPV+) group was significantly younger (median age, 59 v 64 years; P = .001). Compared with HPV-negative (HPV–) vSCCs, HPV+ tumors showed more frequent pathogenic alterations in PIK3CA (31% v 16%; P = .004), PTEN (14% v 2%; P < .0001), EP300 (14% v 1%; P < .0001), STK11 (14% v 1%; P < .0001), AR (5% v 0%; P = .006), and FBXW7 (10% v 3%; P = .03). In contrast, HPV– vSCCs showed more alterations in TP53 (83% v 6%; P < .0001), TERTp (71% v 9%; P < .0001), CDKN2A (55% v 2%; P < .0001), CCND1 amplification (22% v 2%; P < .0001), FAT1 (25% v 4%; P < .0001), NOTCH1 (19% v 6%; P = .002), and EGFR amplification (11% v 0%; P < .0001), as well as a higher rate of 9p24.1 ( PDL1/PDL2) amplification (5% v 1%) and PD-L1 immunohistochemistry high-positive tumor staining (33% v 9%; P = .04). CONCLUSION Comprehensive molecular profiles of vSCC vary considerably with hrHPV status and may inform patient selection into clinical trials. Sixty-one percent of HPV+ vSCCs had a pathogenic alteration in the PI3K/mTOR pathway, whereas HPV– vSCCs showed alterations in TP53, TERTp, CDKN2A, CCND1, and EGFR, and biomarkers associated with responsiveness to immunotherapy.
Health-related quality of life after vascular surgery and endovascular treatment in subjects with critical limb ischemia
Authors: Khalil Emced , Ozcan Sedat 
Published: June 2020

Background & Objective: Revascularization of the target vessel and restoration of distal flow is critical not only to reduce mortality and morbidity but also improve health-related quality of life (HRQoL) in patients with critical limb ischemia. However, data concerning the impact of surgical bypass and percutaneous transluminal angioplasty (PTA) on HRQoL is limited. This study aimed to compare the impact of surgical bypass and PTA on HRQoL in subjects undergoing superficial femoral artery (SFA) or popliteal artery revascularization.
Methods: Seventy-one subjects who underwent successful revascularization of the SFA or popliteal artery either with surgical revascularization or PTA were enrolled in this study. Three months after revascularization, all subjects underwent ankle-brachial index (ABI) measurement, 6-minute walking test and completed the Nottingham Health Profile (NHP) questionnaire. The NHP score differences (measured at the post-procedural 3rd month) between subjects undergoing surgical or endovascular (PTA) revascularization subjects was the primary outcome measure of the study.
Results: Both groups experienced significant improvements in ABI and 6-minute walking distance at post-procedure three months. NHP total scores of Part I and Part II at post-procedural six months were similar in the two groups. However, social isolation [77.98 (0 - 85) vs. 22.53 (0 - 100), p=0.002] and physical abilities [78.7 (30.31 - 87.7) vs. 54.47 (0 - 100), P=0.014] domain scores of the surgical revascularization group were significantly higher than that of the endovascular treatment group.
Conclusion: This study shows that total scores obtained from the NHP questionnaire applied three months after revascularization of SFA stenosis are similar in subjects undergoing surgical revascularization or PTA. However, the social isolation and physical abilities domains of the NHP are significantly higher in subjects receiving surgical revascularization compared to those receiving PTA.
doi: https://doi.org/10.12669/pjms.36.5.2680
How to cite this:Khalil E, Ozcan S. Health-related quality of life after vascular surgery and endovascular treatment in subjects with critical limb ischemia. Pak J Med Sci. 2020;36(5):877-883. doi: https://doi.org/10.12669/pjms.36.5.2680
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Automatic migraine classification using artificial neural networks
Authors: Sanchez-Sanchez Paola A. , García-González José Rafael , Rúa Ascar Juan Manuel 
Published: June 2020

Background: Previous studies of migraine classification have focused on the analysis of brain waves, leading to the development of complex tests that are not accessible to the majority of the population. In the early stages of this pathology, patients tend to go to the emergency services or outpatient department, where timely identification largely depends on the expertise of the physician and continuous monitoring of the patient. However, owing to the lack of time to make a proper diagnosis or the inexperience of the physician, migraines are often misdiagnosed either because they are wrongly classified or because the disease severity is underestimated or disparaged. Both cases can lead to inappropriate, unnecessary, or imprecise therapies, which can result in damage to patients’ health. Methods: This study focuses on designing and testing an early classification system capable of distinguishing between seven types of migraines based on the patient’s symptoms. The methodology proposed comprises four steps: data collection based on symptoms and diagnosis by the treating physician, selection of the most relevant variables, use of artificial neural network models for automatic classification, and selection of the best model based on the accuracy and precision of the diagnosis. Results: The neural network models used provide an excellent classification performance, with accuracy and precision levels >97% and which exceed the classifications made using other model, such as logistic regression, support vector machines, nearest neighbor, and decision trees. Conclusions: The implementation of migraine classification through neural networks is a powerful tool that reduces the time to obtain accurate, reliable, and timely clinical diagnoses.
A mouse model for functional dissection of TAB1 O-GlcNAcylation
Authors: Authier Florence , Muha Villő , van Aalten Daan M.F. 
Published: June 2020

Background: O-GlcNAcylation is a posttranslational modification associated with various physiological and pathophysiological processes including diabetes, cancer, neurodegeneration and inflammation. However, the biological mechanisms underlying the role of specific O-GlcNAc sites and their link to phenotypes remain largely unexplored due to lack of suitable in vivo models. TGF-β activated kinase-1 binding protein-1 (TAB1) is a scaffolding protein required for TGF-β activated kinase-1 (TAK1) mediated signalling. A single O-GlcNAc site has been identified on human TAB1 that modulates TAK1-mediated cytokine release in cells. Methods: Here, we report the generation of the Tab1S393A mouse model using a constitutive knock-in strategy. The Tab1S393A mice carry a Ser393Ala (S393A) mutation that leads to loss of O-GlcNAcylation site on TAB1. Results: We did not observe any obvious phenotype in Tab1S393A mice. Loss of O-GlcNAcylation on TAB1 has no consequences on TAB1 protein level or on TAB1-TAK1 interaction. Conclusions: The homozygous Tab1S393A mice are viable and develop with no obvious abnormalities, providing a powerful tool to further investigate the role of O-GlcNAc on TAB1 in the inflammatory response in the context of a whole organism.
Biomechanical evaluation of a novel double rip-stop technique with medial row knots for rotator cuff repair
Authors: Wang Zhanwen , Li Hong , Long Zeling , Lin Subin , Thoreson Andrew R. , Moran Steven L. , Gingery Anne , Amadio Peter C. , Steinmann Scott P. , Zhao Chunfeng 
Published: June 2020

AimsMany biomechanical studies have shown that the weakest biomechanical point of a rotator cuff repair is the suture-tendon interface at the medial row. We developed a novel double rip-stop (DRS) technique to enhance the strength at the medial row for rotator cuff repair. The objective of this study was to evaluate the biomechanical properties of the DRS technique with the conventional suture-bridge (SB) technique and to evaluate the biomechanical performance of the DRS technique with medial row knots.MethodsA total of 24 fresh-frozen porcine shoulders were used. The infraspinatus tendons were sharply dissected and randomly repaired by one of three techniques: SB repair (SB group), DRS repair (DRS group), and DRS with medial row knots repair (DRSK group). Specimens were tested to failure. In addition, 3 mm gap formation was measured and ultimate failure load, stiffness, and failure modes were recorded.ResultsThe mean load to create a 3 mm gap formation in the DRSK and DRS groups was significantly higher than in the SB group. The DRSK group had the highest load to failure with a mean ultimate failure load of 395.0 N (SD 56.8) compared to the SB and DRS groups, which recorded 147.1 N (SD 34.3) and 285.9 N (SD 89.8), respectively (p < 0.001 for both). The DRS group showed a significantly higher mean failure load than the SB group (p = 0.006). Both the DRS and DRSK groups showed significantly higher mean stiffness than the SB group.ConclusionThe biomechanical properties of the DRS technique were significantly improved compared to the SB technique. The DRS technique with medial row knots showed superior biomechanical performance than the DRS technique alone.
High-stability, high-beam-quality, and pulse-width-tunable 1319  nm laser system for VISAR applications in high-power laser facilities
Authors: Xiao Qi , Pan Xue , Guo Jiangtao , Wang Xiaoqin , Wang Jiangfeng , Jiang Xiuqing , Li Guoyang , Lu Xinghua , Wang Xiaochao , Zhou Shenlei , Li Xuechun 
Published: June 2020

A high-energy, high-beam-quality, and pulse-width-tunable Nd:YAG laser system, pumped by vertical cavity surface emitting laser arrays and laser diodes, is demonstrated and applied to a velocity interferometry system for any reflector (VISAR) application in a high power laser facility. A multistage multipass amplification structure is used to fully extract the amplifier energy and obtain a high-energy pulse. The temporal waveform is compensated to provide a square waveform, with a flatness less than 8% (peak-to-peak value). The peak power is greater than 100 kW with a frequency-doubling efficiency of 25% for a 50 ns pulse width. The laser operates as a single shot with 1–5 Hz repetition frequency and 0.7% rms energy stability.
Discurso e Imagem, Ekphrasis e Retrato: Algumas Fontes Teóricas Antigas
Authors: Agnolon Alexandre 
Published: June 2020

O artigo trata da relação entre a preceptística retórica antiga – particularmente doutrinas relacionadas às imagines – e a composição literária antiga; mediante exemplos de Progymnasmata, uma série de tratados escolares de autoria de vários rétores gregos, como Aftônio, Hermógenes e Teão, discute a construção de imagens verbais e a importância desses exercícios escolares para a formação de oradores e poetas; e, além disso, arrola outros exemplos, retirados da Retórica a Herênio, de autoria anônima, do Sobre a Invenção de Cícero, do Tratado da Imitação de Dionísio de Halicarnasso e das Instituições Oratórias de Quintiliano. O objetivo fundamental do artigo é demonstrar três pontos de fundamental importância: a) que a imagem, na chave do exemplum, demonstra a proximidade das artes no que se refere a seus processos de composição que, por seu turno, associam-se ao binômio imitação/emulação; b) que, como não eram simples ornato, as imagens verbais poderiam contribuir positivamente para a eficácia do discurso, uma vez que, geradoras de pathos, convertiam-se em estratégia elocutiva eficiente para a causa; e c) o uso recorrente da Ekphrasis e do Retrato na prática de prosadores e poetas helenísticos e romanos.
Colonic Myeloid Sarcoma as a Rare Presentation of Relapsed Acute Myeloid Leukemia
Authors: Phatharacharukul Parkpoom , Fayad Nabil , Siwiec Robert 
Published: June 2020

ABSTRACT
Myeloid sarcoma (MS), an extramedullary tumor of immature granulocytic cells, affects the gastrointestinal tract in approximately 10% of cases. MS involvement of the colon and rectum is considered to be extremely rare. We present a 36-year-old woman with acute myeloid leukemia and allogenic hematopoietic stem cell transplant 2 years before who was admitted with abdominal pain and nonmucous, nonbloody diarrhea. Colonoscopy revealed an ulcerated mass in the proximal colon, and biopsies showed MS compatible with acute myeloid leukemia relapse.
COVID-19 (coronavirus): un peligro emergente que requiere abordaje en las organizaciones
Authors: Rodríguez-Rojas Yuber Liliana 
Published: June 2020

El virus covid-19 surgió en diciembre en China (Zhao et al., 2020). Este es un tipo de coronavirus que se circula entre los murciélagos. De acuerdo con los reportes, apareció en el mercado de Wuhan (Trilla, 2020). La familia coronavirus puede causar resfriado común, el síndrome respiratorio agudo grave (también conocido como sars, por sus siglas en inglés), y el síndrome respiratorio de Oriente Medio (también conocido como mers, por sus siglas en inglés). Su capa externa tiene la forma de una corona; por esta razón recibe la denominación de coronavirus.
C-Terminal HA Tags Compromise Function and Exacerbate Phenotypes ofSaccharomyces cerevisiaeBloom’s Helicase Homolog Sgs1 SUMOylation-Associated Mutants
Authors: Cohen Matan , Lichten Michael 
Published: June 2020

AbstractThe Sgs1 helicase and Top3-Rmi1 decatenase form a complex that affects homologous recombination outcomes during the mitotic cell cycle and during meiosis. Previous studies have reported that Sgs1-Top3-Rmi1 function is regulated by SUMOylation that is catalyzed by the Smc5-Smc6-Mms21 complex. These studies used strains in which SGS1 was C-terminally tagged with three or six copies of a human influenza hemagglutinin-derived epitope tag (3HA and 6HA). They identified SGS1 mutants that affect its SUMOylation, which we will refer to as SGS1 SUMO-site mutants. In previous work, these mutants showed phenotypes consistent with substantial loss of Sgs1-Top3-Rmi1 function during the mitotic cell cycle. We find that the reported phenotypes are largely due to the presence of the HA epitope tags. Untagged SGS1 SUMO-site mutants show either wild-type or weak hypomorphic phenotypes, depending on the assay. These phenotypes are exacerbated by both 6HA and 3HA epitope tags in two different S. cerevisiae strain backgrounds. Importantly, a C-terminal 6HA tag confers strong hypomorphic or null phenotypes on an otherwise wild-type Sgs1 protein. Taken together, these results suggest that the HA epitope tags used in previous studies seriously compromise Sgs1 function. Furthermore, they raise the possibilities either that sufficient SUMOylation of the Sgs1-Top3-Rmi1 complex might still occur in the SUMO-site mutants isolated, or that Smc5-Smc6-Mms21-mediated SUMOylation plays a minor role in the regulation of Sgs1-Top3-Rmi1 during recombination.
Analysis of the Charles De Gaulle Aircraft Carrier Covid19 Epidemic: Infectivity and Fatality in the Young, Healthy, Active Population
Authors: Boretti Alberto 
Published: June 2020

The case of the Charles De Gaulle aircraft carrier Covid19 outbreak indicates those young, healthy and active, apart from very few exceptions, do not get infected even if challenged, or are only very mild or asymptomatic if infected. As per April 20, 2020, of almost 2,000 people challenged, 1,081 got infected. Of the 1,081, only 24 ended up in a hospital. Of the 24, only 1 was reported in need of intensive care. As per April 29, 2020, only 5 were still in the hospital, and 1 was in intensive care. As per May 4, 2020, there were only 2 still in the hospital, 1 of them in need of intensive care. On May 11, 2020, only the 1 previously in intensive care was still hospitalized but out of intensive care. Thus, infectivity and fatality are much lower than thought for the young, healthy, active population