bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bulk and surface topological indices for a skyrmion string: current-driven dynamics of skyrmion string in stepped samples
Authors: Koshibae Wataru , Nagaosa Naoto 
Published: November 2020

AbstractThe magnetic skyrmion is a topological magnetic vortex, and its topological nature is characterized by an index called skyrmion number which is a mapping of the magnetic moments defined on a two-dimensional space to a unit sphere. In three-dimensions, a skyrmion, i.e., a vortex penetrating though the magnet naturally forms a string, which terminates at the surfaces of the magnet or in the bulk. For such a string, the topological indices, which control its topological stability are less trivial. Here, we study theoretically, in terms of numerical simulation, the dynamics of current-driven motion of a skyrmion string in a film sample with the step edges on the surface. In particular, skyrmion–antiskyrmion pair is generated by driving a skyrmion string through the side step with an enough height. We find that the topological indices relevant to the stability are the followings; (1) skyrmion number along the developed surface, and (2) the monopole charge in the bulk defined as the integral over the surface enclosing a singular magnetic configuration. As long as the magnetic configuration is slowly varying, the former is conserved while its changes is associated with nonzero monopole charge. The skyrmion number and the monoplole charge offer a coherent understanding of the stability of the topological magnetic texture and the nontrivial dynamics of skyrmion strings.
Modular affinity-labeling of the cytosine demethylation base elements in DNA
Authors: Wang Fanny , Zahid Osama K. , Ghanty Uday , Kohli Rahul M. , Hall Adam R. 
Published: November 2020

Abstract5-methylcytosine is the most studied DNA epigenetic modification, having been linked to diverse biological processes and disease states. The elucidation of cytosine demethylation has drawn added attention the three additional intermediate modifications involved in that pathway—5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine—each of which may have distinct biological roles. Here, we extend a modular method for labeling base modifications in DNA to recognize all four bases involved in demethylation. We demonstrate both differential insertion of a single affinity tag (biotin) at the precise position of target elements and subsequent repair of the nicked phosphate backbone that remains following the procedure. The approach enables affinity isolation and downstream analyses without inducing widespread damage to the DNA.
CMT-3 targets different α-synuclein aggregates mitigating their toxic and inflammogenic effects
Authors: González-Lizárraga Florencia , Ploper Diego , Ávila César L. , Socías Sergio B. , dos-Santos-Pereira Mauricio , Machín Belén , Del-Bel Elaine , Michel Patrick Pierre , Pietrasanta Lía I. , Raisman-Vozari Rita , Chehín Rosana 
Published: November 2020

AbstractParkinson's disease (PD) is a neurodegenerative disorder for which only symptomatic treatments are available. Repurposing drugs that target α-synuclein aggregation, considered one of the main drivers of PD progression, could accelerate the development of disease-modifying therapies. In this work, we focused on chemically modified tetracycline 3 (CMT-3), a derivative with reduced antibiotic activity that crosses the blood–brain barrier and is pharmacologically safe. We found that CMT-3 inhibited α-synuclein amyloid aggregation and led to the formation of non-toxic molecular species, unlike minocycline. Furthermore, CMT-3 disassembled preformed α-synuclein amyloid fibrils into smaller fragments that were unable to seed in subsequent aggregation reactions. Most interestingly, disaggregated species were non-toxic and less inflammogenic on brain microglial cells. Finally, we modelled the interactions between CMT-3 and α-synuclein aggregates by molecular simulations. In this way, we propose a mechanism for fibril disassembly. Our results place CMT-3 as a potential disease modifier for PD and possibly other synucleinopathies.
Positron annihilation localization by nanoscale magnetization
Authors: Gholami Yaser H. , Yuan Hushan , Wilks Moses Q. , Josephson Lee , El Fakhri Georges , Normandin Marc D. , Kuncic Zdenka 
Published: November 2020

AbstractIn positron emission tomography (PET), the finite range over which positrons travel before annihilating with an electron places a fundamental physical limit on the spatial resolution of PET images. After annihilation, the photon pair detected by the PET instrumentation is emitted from a location that is different from the positron-emitting source, resulting in image blurring. Here, we report on the localization of positron range, and hence annihilation quanta, by strong nanoscale magnetization of superparamagnetic iron oxide nanoparticles (SPIONs) in PET-MRI. We found that positron annihilations localize within a region of interest by up to 60% more when SPIONs are present (with [Fe] = 3 mM) compared to when they are not. The resulting full width at half maximum of the PET scans showed the spatial resolution improved by up to $$\approx$$
≈
30%. We also found evidence suggesting that the radiolabeled SPIONs produced up to a six-fold increase in ortho-positronium. These results may also have implications for emerging cancer theranostic strategies, where charged particles are used as therapeutic as well as diagnostic agents and improved dose localization within a tumor is a determinant of better treatment outcomes.
Arbuscular mycorrhizal fungi favor invasive Echinops sphaerocephalus when grown in competition with native Inula conyzae
Authors: Řezáčová Veronika , Řezáč Milan , Gryndlerová Hana , Wilson Gail W. T. , Michalová Tereza 
Published: November 2020

AbstractIn a globalized world, plant invasions are common challenges for native ecosystems. Although a considerable number of invasive plants form arbuscular mycorrhizae, interactions between arbuscular mycorrhizal (AM) fungi and invasive and native plants are not well understood. In this study, we conducted a greenhouse experiment examining how AM fungi affect interactions of co-occurring plant species in the family Asteracea, invasive Echinops sphaerocephalus and native forb of central Europe Inula conyzae. The effects of initial soil disturbance, including the effect of intact or disturbed arbuscular mycorrhizal networks (CMNs), were examined. AM fungi supported the success of invasive E. sphaerocephalus in competition with native I. conyzae, regardless of the initial disturbance of CMNs. The presence of invasive E. sphaerocephalus decreased mycorrhizal colonization in I. conyzae, with a concomitant loss in mycorrhizal benefits. Our results confirm AM fungi represent one important mechanism of plant invasion for E. sphaerocephalus in semi-natural European grasslands.
Age-related dissociation of N400 effect and lexical priming
Authors: Tiedt Hannes O. , Ehlen Felicitas , Klostermann Fabian 
Published: November 2020

AbstractThe use of contextual information is an important capability to facilitate language comprehension. This can be shown by studying behavioral and neurophysiological measures of accelerated word recognition when semantically or phonemically related information is provided in advance, resulting in accompanying attenuation of the respective event-related potential, i.e. the N400 effect. Against the background of age-dependent changes in a broad variety of lexical capacities, we aimed to study whether word priming is accomplished differently in elderly compared to young persons. 19 young (29.9 ± 5.6 years) and 15 older (69.0 ± 7.2 years) healthy adults participated in a primed lexical decision task that required the classification of target stimuli (words or pseudo-words) following related or unrelated prime words. We assessed reaction time, task accuracy and N400 responses. Acceleration of word recognition by semantic and phonemic priming was significant in both groups, but resulted in overall larger priming effects in the older participants. Compared with young adults, the older participants were slower and less accurate in responding to unrelated word-pairs. The expected N400 effect was smaller in older than young adults, particularly during phonemic word and pseudo-word priming, with a rather similar N400 amplitude reduction by semantic relatedness. The observed pattern of results is consistent with preserved or even enhanced lexical context sensitivity in older compared to young adults. This, however, appears to involve compensatory cognitive strategies with higher lexical processing costs during phonological processing in particular, suggested by a reduced N400 effect in the elderly.
A simple score derived from bone marrow immunophenotyping is important for prognostic evaluation in myelodysplastic syndromes
Authors: Vido-Marques J. R. , Reis-Alves S. C. , Saad S. T. O. , Metze K. , Lorand-Metze I. 
Published: November 2020

AbstractImmunophenotyping of bone marrow (BM) precursors has been used as an ancillary diagnostic tool in myelodysplastic syndromes (MDS), but there is no general agreement about which variables are the most relevant for prognosis. We developed a parsimonious prognostic model based on BM cell populations well-defined by phenotype. We analyzed 95 consecutive patients with primary MDS diagnosed at our Institution between 2005 and 2012 where BM immunophenotyping had been performed at diagnosis. Median follow-up: 42 months (4–199). Median age: 67 years (33–79). According to IPSS-R, 71 cases were low or intermediate risk. Flow variables significant in the univariate Cox analysis: “%monocytes/TNCs”, “% CD16+monocytes/TNCs”, “total alterations in monocytes”, “% myeloid CD34+cells”, “number of abnormal expressions in myeloblasts” and “% of B-cell progenitors”. In the multivariate model remained independent: “% myeloid CD34+cells”, B-cell progenitors” and “% CD16+monocytes/TNCs”. These variables were categorized by the extreme quartile risk ratio strategy in order to build the score: % myeloid CD34+cells” (≥ 2.0% = 1 point), B-cell progenitors” (< 0.05% 1 point) and “CD16+monocytes/TNCs” (≥ 1.0% 1 point). This score could separate patients with a different survival. There was a weak correlation between the score and IPSS-R. Both had independent prognostic values and so, the flow score adds value for the prognostic evaluation in MDS.
Association between obesity and education level among the elderly in Taipei, Taiwan between 2013 and 2015: a cross-sectional study
Authors: Hsieh Tsai-Hao , Lee Jason Jiunshiou , Yu Ernest Wen-Ruey , Hu Hsiao-Yun , Lin Shu-Yi , Ho Chin-Yu 
Published: November 2020

AbstractThe inverse association between obesity and education level has been demonstrated in many developed countries; however, few studies have investigated obesity in geriatric populations. This cross-sectional analysis explored the association between geriatric obesity and education level, along with other demographic characteristics in Taipei, Taiwan between 2013 and 2015. Taipei citizens ≥ 65 years (aborigines ≥ 55 years) were recruited to participate in the elderly health examination programme. Logistic regression was applied to analyse the relationship between obesity (defined as body mass index ≥ 27 kg/m2 in Taiwan) and education level among men and women after controlling for age, race, income status, and smoking status. A total of 28,092 men and 31,835 women were included in the final analysis. Compared to those with education years ≥ 16, older men and women with education years ≤ 12 had higher odds of being obese. The odds ratios increase as years of education decrease, and the trend is more pronounced among women. Aborigines had much higher chances of being obese among men and women, while there were no differences by income status. The results clarified the factors related to obesity in the elderly, and will be useful for authorities working to improve health outcomes among this population.
Novel viruses detected in bats in the Republic of Korea
Authors: Lee Sook-Young , Chung Chul-Un , Park Jun Soo , Oem Jae-Ku 
Published: November 2020

AbstractBats are natural reservoirs for potential zoonotic viruses. In this study, next-generation sequencing was performed to obtain entire genome sequences of picornavirus from a picornavirus-positive bat feces sample (16BF77) and to explore novel viruses in a pooled bat sample (16BP) from samples collected in South Korea, 2016. Fourteen mammalian viral sequences were identified from 16BF77 and 29 from 16BP, and verified by RT-PCR. The most abundant virus in 16BF77 was picornavirus. Highly variable picornavirus sequences encoding 3Dpol were classified into genera Kobuvirus, Shanbavirus, and an unassigned group within the family Picornaviridae. Amino acid differences between these partial 3Dpol sequences were ≥ 65.7%. Results showed that one bat was co-infected by picornaviruses of more than two genera. Retrovirus, coronavirus, and rotavirus A sequences also were found in the BP sample. The retrovirus and coronavirus genomes were identified in nine and eight bats, respectively. Korean bat retroviruses and coronavirus demonstrated strong genetic relationships with a Chinese bat retrovirus (RfRV) and coronavirus (HKU5-1), respectively. A co-infection was identified in one bat with a retrovirus and a coronavirus. Our results indicate that Korean bats were multiply infected by several mammal viruses.
Biceps brachii muscle hardness assessed by a push-in meter in comparison to ultrasound strain elastography
Authors: Murayama Mitsuyoshi , Nosaka Kazunori , Inami Takayuki , Shima Norihiro , Yoneda Tsugutake 
Published: November 2020

AbstractThis study investigated the relationship between push-in meter (PM) and ultrasound strain elastography (USE) for biceps brachii (BB) muscle hardness. BB hardness of 21 young men was assessed by PM and USE during rest and isometric contractions of six different intensities (15, 30, 45, 60, 75, 90% of maximal voluntary contraction: MVC) at 30°, 60° and 90° elbow flexion. Muscle hardness (E) was calculated from the force–displacement relationship in PM, and strain ratio (SR) between an acoustic coupler (elastic modulus: 22.6 kPa) and different regions of interest (ROIs) in BB was calculated and converted to Young’s modulus (YM) in USE. In resting muscle, E was 26.1 ± 6.4 kPa, and SR and YM for the whole BB was 0.88 ± 0.4 and 30.8 ± 12.8 kPa, respectively. A significant (p < 0.01) correlation was evident between E and logarithmical transformed SR (LTSR) for the ROI of whole BB (r = − 0.626), and E and converted YM (r = 0.615). E increased approximately ninefold from resting to 90% MVC, and E and LTSR (r = − 0.732 to − 0.880), and E and converted YM for the SR above 0.1 were correlated (r = 0.599–0.768, p < 0.01). These results suggest that muscle hardness values obtained by PM and USE are comparable.
Four-month operational heat acclimatization positively affects the level of heat tolerance 6 months later
Authors: Malgoyre Alexandra , Siracusa Julien , Tardo-Dino Pierre-Emmanuel , Garcia-Vicencio Sebastian , Koulmann Nathalie , Epstein Yoram , Charlot Keyne 
Published: November 2020

AbstractBenefits obtained after heat acclimation/acclimatization should be completely lost after an estimated period of 6 weeks. However, this estimate is still hypothetical. We evaluate the long-term effects of heat acclimatization on the level of heat tolerance. Physiological and subjective markers of heat tolerance were assessed during a heat stress test (HST: 3 × 8-min runs outdoors [~ 40 °C and 20% RH] at 50% of their estimated speed at VO2max) performed on the 2nd day upon arrival to the desert military base in the United Arab Emirates after a first day of mostly passive exposure to heat. Among the 50 male French soldiers, 25 partook in a 4-month military mission in countries characterized by a hot environment ~ 6 months prior to the study (HA). The other 25 participants were never heat acclimatized (CT). Rectal temperature (p = 0.023), heart rate (p = 0.033), and perceived exertion (p = 0.043) were lower in the HA than CT group at the end of HST. Soldiers who experienced a former 4-month period of natural heat acclimatization very likely had a higher level of heat tolerance during exercise in the heat, even 6 months after returning from the previous desert mission, than that of their non-acclimatized counterparts.
Toward a better multi-model ensemble prediction of East Asian and Australasian precipitation during non-mature ENSO seasons
Authors: Sohn Soo-Jin , Kim WonMoo 
Published: November 2020

AbstractAn effective and reliable way for better predicting the seasonal Australasian and East Asian precipitation variability in a multi-model ensemble (MME) prediction system is newly designed, in relation to the performance of predicting El Niño-Southern Oscillation (ENSO) and its impact. While ENSO is a major predictability source of global and regional precipitation variation, the prediction skill of precipitation is not solely due to typical ENSO alone, of which variability and predictability exhibit strong seasonality. The first mode of ENSO variability has large variance with high prediction skill for boreal winter and small variance with low skill for spring and summer, while the second mode shows the opposite phase. The regional prediction skills for Australasian and East Asian precipitation also show such seasonal dependence, with low skill and large spread of individual models’ skills during the boreal spring to summer and high skill and small spread during winter. Using the individual models’ reproducibility of the association between ENSO and regional precipitation, the prediction skills of the MME with selected models can improve at regional levels, compared to those for all-inclusive MME, during boreal spring to summer. While typical ENSO as a predictability source may still dominate during boreal winter, consideration of complex ENSO structure and its diverse impact can lead to a better prediction of regional precipitation variability during non-mature phase of ENSO seasons.
Synthesis of epitaxial monolayer Janus SPtSe
Authors: Sant Roberto , Gay Maxime , Marty Alain , Lisi Simone , Harrabi Rania , Vergnaud Céline , Dau Minh Tuan , Weng Xiaorong , Coraux Johann , Gauthier Nicolas , Renault Olivier , Renaud Gilles , Jamet Matthieu 
Published: November 2020

AbstractJanus single-layer transition metal dichalcogenides, in which the two chalcogen layers have a different chemical nature, push chemical composition control beyond what is usually achievable with van der Waals heterostructures. Here, we report such a Janus compound, SPtSe, which is predicted to exhibit strong Rashba spin–orbit coupling. We synthetized it by conversion of a single-layer of PtSe2 on Pt(111) via sulfurization under H2S atmosphere. Our in situ and operando structural analysis with grazing incidence synchrotron X-ray diffraction reveals the process by which the Janus alloy forms. The crystalline long-range order of the as-grown PtSe2 monolayer is first lost due to thermal annealing. A subsequent recrystallization in presence of a source of sulfur yields a highly ordered SPtSe alloy, which is isostructural to the pristine PtSe2. The chemical composition is resolved, layer-by-layer, using angle-resolved X-ray photoelectron spectroscopy, demonstrating that Se-by-S substitution occurs selectively in the topmost chalcogen layer.
New insights in the coordinated amidase and glucosaminidase activity of the major autolysin (Atl) in Staphylococcus aureus
Authors: Nega Mulugeta , Tribelli Paula Maria , Hipp Katharina , Stahl Mark , Götz Friedrich 
Published: November 2020

AbstractAfter bacterial cell division, the daughter cells are still covalently interlinked by the peptidoglycan network which is resolved by specific hydrolases (autolysins) to release the daughter cells. In staphylococci, the major autolysin (Atl) with its two domain enzymes, N-acetylmuramyl-L-alanine amidase (AmiA) and β-N-acetylglucosaminidase (GlcA), resolves the peptidoglycan to release the daughter cells. Internal deletions in each of the enzyme domains revealed defined morphological alterations such as cell cluster formation in ΔamiA, ΔglcA and Δatl, and asymmetric cell division in the ΔglcA. A most important finding was that GlcA activity requires the prior removal of the stem peptide by AmiA for its activity thus the naked glycan strand is its substrate. Furthermore, GlcA is not an endo-β-N-acetylglucosaminidase but an exo-enzyme that cuts the glycan backbone to disaccharides independent of its O-acetylation modification. Our results shed new light into the sequential peptidoglycan hydrolysis by AmiA and GlcA during cell division in staphylococci.
FRA1 is essential for the maintenance of the oncogenic phenotype induced by in vitro long-term arsenic exposure
Authors: Barguilla Irene , Bach Jordi , Peremartí Jana , Marcos Ricard , Hernández Alba 
Published: November 2020

Abstract
Arsenic induces oncogenic effects activating stress-related signalling pathways. This can result in the over-activation of the AP-1 protein, specifically its FRA1 component. FRA1 is a transcription factor frequently overexpressed in epithelial tumors, where it can regulate the expression of different target genes. Accordingly, FRA1 could play an essential role in the in vitro cell transformation induced by arsenic. FRA1 levels were monitored in MEF cells throughout their transformation stages during 40 weeks of long-term 2 μM arsenic exposure. Interestingly, the results show a progressive FRA1 overexpression with time (60-fold and 11-fold for mRNA and pFRA/non-pFRA1, respectively, at week 40), which may be responsible for the observed altered expression in the FRA1 downstream target genes Pten, Pdcd4, Tpm1, Tgfb1, Tgfb2, Zeb1, Zeb2, and Twist. The levels of MAPKs (ERK, p38, and JNK) and other known players upstream from FRA1 were assessed at equivalent time-points, and ERK, p38 and RAS were pinpointed as potential candidates involved in arsenic-induced FRA1 activation. Furthermore, FRA1 stable knockdown under chronic arsenic exposure settings elicits a remarkable impact on the features relative to the cells’ oncogenic phenotype. Notably, FRA1 knockdown cells present a 30% diminished proliferation rate, a 50% lowered migration and invasion potential, a 50% reduction in senescence, and a 30–60% reduced tumorsphere-forming ability. This work is the first to demonstrate the important role of FRA1 in the development and aggressiveness of the in vitro transformed phenotype induced by long-term arsenic exposure.
Anesthetic Management of a Child with β-Thalassemia Major and Cortical Venous Thrombosis
Authors: Tyagi Mayank , Dube Surya K. , Rajagopalan Vanitha , Singh Gyaninder P. 
Published: November 2020

Abstractβ-thalassemia are a group of inherited blood disorders with reduced hemoglobin levels. β-thalassemia major is the severe form of disease, and the patients often display an array of associated organ dysfunction which thus increase the risk associated with surgery and anesthesia. Patients with β-thalassemia major can have multiple pathological defects that may lead to thromboembolic events. Here, we report such a case who was complicated by occurrence of cerebral sinus thrombosis and presented for decompressive hemicraniectomy under general anesthesia. The anesthetic challenges during in such scenario have been discussed.