bluuebunny/crossref_metadata_2025_split huggingface dataset:
Striking essential oil: tapping into a largely unexplored source for drug discovery
Authors: Feyaerts Adam F. , Luyten Walter , Van Dijck Patrick 
Published: February 2020

AbstractEssential oils (EOs) have been used therapeutically for centuries. In recent decades, randomized controlled (clinical) trials have supported efficacy in specific therapeutic indications for a few of them. Some EOs, their components or derivatives thereof have been approved as drugs. Nevertheless, they are still considered products that are mainly used in complementary and alternative medicine. EO components occupy a special niche in chemical space, that offers unique opportunities based on their unusual physicochemical properties, because they are typically volatile and hydrophobic. Here we evaluate selected physicochemical parameters, used in conventional drug discovery, of EO components present in a range of commercially available EOs. We show that, contrary to generally held belief, most EO components meet current-day requirements of medicinal chemistry for good drug candidates. Moreover, they also offer attractive opportunities for lead optimization or even fragment-based drug discovery. Because their therapeutic potential is still under-scrutinized, we propose that this be explored more vigorously with present-day methods.
Disentangling the taxonomy of the subfamily Rasborinae (Cypriniformes, Danionidae) in Sundaland using DNA barcodes
Authors: Sholihah Arni , Delrieu-Trottin Erwan , Sukmono Tedjo , Dahruddin Hadi , Risdawati Renny , Elvyra Roza , Wibowo Arif , Kustiati Kustiati , Busson Frédéric , Sauri Sopian , Nurhaman Ujang , Dounias Edmond , Zein Muhamad Syamsul Arifin , Fitriana Yuli , Utama Ilham Vemendra , Muchlisin Zainal Abidin , Agnèse Jean-François , Hanner Robert , Wowor Daisy , Steinke Dirk , Keith Philippe , Rüber Lukas , Hubert Nicolas 
Published: February 2020

AbstractSundaland constitutes one of the largest and most threatened biodiversity hotspots; however, our understanding of its biodiversity is afflicted by knowledge gaps in taxonomy and distribution patterns. The subfamily Rasborinae is the most diversified group of freshwater fishes in Sundaland. Uncertainties in their taxonomy and systematics have constrained its use as a model in evolutionary studies. Here, we established a DNA barcode reference library of the Rasborinae in Sundaland to examine species boundaries and range distributions through DNA-based species delimitation methods. A checklist of the Rasborinae of Sundaland was compiled based on online catalogs and used to estimate the taxonomic coverage of the present study. We generated a total of 991 DNA barcodes from 189 sampling sites in Sundaland. Together with 106 previously published sequences, we subsequently assembled a reference library of 1097 sequences that covers 65 taxa, including 61 of the 79 known Rasborinae species of Sundaland. Our library indicates that Rasborinae species are defined by distinct molecular lineages that are captured by species delimitation methods. A large overlap between intraspecific and interspecific genetic distance is observed that can be explained by the large amounts of cryptic diversity as evidenced by the 166 Operational Taxonomic Units detected. Implications for the evolutionary dynamics of species diversification are discussed.
Rewilding processes shape the use of Mediterranean landscapes by an avian top scavenger
Authors: Martin-Díaz P. , Cortés-Avizanda A. , Serrano D. , Arrondo E. , Sánchez-Zapata J. A. , Donázar J. A. 
Published: February 2020

AbstractThe Mediterranean biome has seen a great decline in its rural population. This trend has been followed by an abandonment of agricultural and livestock practices, which has provided an opportunity for rewilding to take place. Rewilding processes can modify the availability of carrion resources for avian obligate scavengers and reduce accessible open areas due to the increase of shrub and forest. We examined how changes in landscape configuration in the past five decades (1956–2011) mediate the foraging behaviour of griffon vultures. Particularly, we examined whether vultures use those areas under natural succession and with a high availability of wild ungulate carcasses. We used GPS information yielded by 30 adult griffon vultures exploiting large regions of southern Spain. We determined (a) habitat use considering land uses and food availability and (b) how tracked individuals responded to areas in different stages of rewilding. Our results showed that vultures preferentially used Mediterranean scrublands, woodlands and the agroforest Mediterranean ecosystem called dehesa, as well as areas with high food resources, namely wild ungulates in winter and a mixture of wild ungulates and livestock in summer. Due to a higher abundance of wild ungulates, vultures forage preferentially in areas with low levels of rewilding, either for being in the first stages of natural succession or for not having experienced further rewilding since the middle of the last century. Rewilding processes are expected to continue in the future affecting the scavenger guild structure and function deeply. Improved management will be essential to preserve ecological processes, ecosystem services and populations of endangered species.
Neural stem cell phenotype of tanycyte-like ependymal cells in the circumventricular organs and central canal of adult mouse brain
Authors: Furube Eriko , Ishii Haruna , Nambu Yuri , Kurganov Erkin , Nagaoka Sumiharu , Morita Mitsuhiro , Miyata Seiji 
Published: February 2020

AbstractTanycyte is a subtype of ependymal cells which extend long radial processes to brain parenchyma. The present study showed that tanycyte-like ependymal cells in the organum vasculosum of the lamina terminalis, subfornical organ and central canal (CC) expressed neural stem cell (NSC) marker nestin, glial fibrillar acidic protein and sex determining region Y. Proliferation of these tanycyte-like ependymal cells was promoted by continuous intracerebroventricular infusion of fibroblast growth factor-2 and epidermal growth factor. Tanycytes-like ependymal cells in the CC are able to form self-renewing neurospheres and give rise mostly to new astrocytes and oligodendrocytes. Collagenase-induced small medullary hemorrhage increased proliferation of tanycyte-like ependymal cells in the CC. These results demonstrate that these tanycyte-like ependymal cells of the adult mouse brain are NSCs and suggest that they serve as a source for providing new neuronal lineage cells upon brain damage in the medulla oblongata.
Crosstalk between DNA methylation and gene expression in colorectal cancer, a potential plasma biomarker for tracing this tumor
Authors: Kerachian Mohammad Amin , Javadmanesh Ali , Azghandi Marjan , Mojtabanezhad Shariatpanahi Afsaneh , Yassi Maryam , Shams Davodly Ehsan , Talebi Amin , Khadangi Fatemeh , Soltani Ghodratollah , Hayatbakhsh Abdorasool , Ghaffarzadegan Kamran 
Published: February 2020

AbstractColorectal cancer (CRC), the second leading cause of cancer mortality, constitutes a significant global health burden. An accurate, noninvasive detection method for CRC as complement to colonoscopy could improve the effectiveness of treatment. In the present study, SureSelectXT Methyl-Seq was performed on cancerous and normal colon tissues andCLDN1,INHBAandSLC30A10were found as candidate methylated genes. MethyLight assay was run on formalin-fixed paraffin-embedded (FFPE) and fresh case and control tissues to validate the methylation of the selected gene. The methylation was significantly different (p-values < 2.2e-16) with a sensitivity of 87.17%; at a specificity cut-off of 100% in FFPE tissues. Methylation studies on fresh tissues, indicated a sensitivity of 82.14% and a specificity cut-off of 92% (p-values = 1.163e-07). The biomarker performance was robust since, normal tissues indicated a significant 22.1-fold over-expression of the selected gene as compared to the corresponding CRC tissues (p-value < 2.2e-16) in the FFPE expression assay. In our plasma pilot study, evaluation of the tissue methylation marker in the circulating cell-free DNA, demonstrated that 9 out of 22 CRC samples and 20 out of 20 normal samples were identified correctly. In summary, there is a clinical feasibility that the offered methylated gene could serve as a candidate biomarker for CRC diagnostic purpose, although further exploration of our candidate gene is warranted.
Response of the human gut and saliva microbiome to urbanization in Cameroon
Authors: Lokmer Ana , Aflalo Sophie , Amougou Norbert , Lafosse Sophie , Froment Alain , Tabe Francis Ekwin , Poyet Mathilde , Groussin Mathieu , Said-Mohamed Rihlat , Ségurel Laure 
Published: February 2020

AbstractUrban populations from highly industrialized countries are characterized by a lower gut bacterial diversity as well as by changes in composition compared to rural populations from less industrialized countries. To unveil the mechanisms and factors leading to this diversity loss, it is necessary to identify the factors associated with urbanization-induced shifts at a smaller geographical scale, especially in less industrialized countries. To do so, we investigated potential associations between a variety of dietary, medical, parasitological and socio-cultural factors and the gut and saliva microbiomes of 147 individuals from three populations along an urbanization gradient in Cameroon. We found that the presence ofEntamoebasp., a commensal gut protozoan, followed by stool consistency, were major determinants of the gut microbiome diversity and composition. Interestingly, urban individuals have retained most of their gut eukaryotic and bacterial diversity despite significant changes in diet compared to the rural areas, suggesting that the loss of bacterial microbiome diversity observed in industrialized areas is likely associated with medication. Finally, we observed a weak positive correlation between the gut and the saliva microbiome diversity and composition, even though the saliva microbiome is mainly shaped by habitat-related factors.
Structural characterization of Kannurin isoforms and evaluation of the role of β-hydroxy fatty acid tail length in functional specificity
Authors: Shabeer Ali H. , Ajesh K. , Dileep K. V. , Prajosh P. , Sreejith K. 
Published: February 2020

AbstractThe novel anti-fungal cyclic lipopeptide ‘Kannurin’ and its three structural variants produced by Bacillus cereus AK1 were previously reported from our laboratory. The present study reports unexplored structural variants of Kannurin those have functional benefits. Due to the difference in β-hydroxy fatty acid tail length, they are designated here as Kannurin A (m/z 994.67 ± 0.015), B (m/z 1008.68 ± 0.017), C (m/z 1022.69 ± 0.021), D (m/z 1036.70 ± 0.01), CL (m/z 1040.71 ± 0.02) and DL (m/z 1054.72 ± 0.01). The isoform A (m/z 994.67 ± 0.015) is the shortest cyclic form of Kannurin identified so far. In addition, CL (m/z 1040.71 ± 0.02) and DL (m/z 1054.72 ± 0.01) are the rare natural linear forms. The results of the antimicrobial assays deduced that the difference in lipid tail length of the isoforms contributes tremendous differences in their antimicrobial properties. The isoforms with short lipid tails (A and B) are more selective and potent towards bacteria, whereas the isoforms with long lipid tails (C and D) are more potent against fungi. The molecular dynamics studies and electron microscopic observations supported with circular dichroic spectroscopy analysis showed the structural confirmation and formation of aggregates of Kannurin in solution. The molecular dynamics simulation studies revealed that a single molecule of Kannurin makes enormous intra-molecular interactions and structural re-arrangements to attain stable lowest energy state in solution. When they reach a particular concentration (CMC) especially in aqueous environment, tends to form structural aggregates called ‘micelles’. With the structural information and activity relationship described in this study, it is trying to point out the sensitive structural entities that can be modified to improve the efficacy and target specificities of lipopeptide class of antibiotics.
Variant phasing and haplotypic expression from long-read sequencing in maize
Authors: Wang Bo , Tseng Elizabeth , Baybayan Primo , Eng Kevin , Regulski Michael , Jiao Yinping , Wang Liya , Olson Andrew , Chougule Kapeel , Buren Peter Van , Ware Doreen 
Published: February 2020

AbstractHaplotype phasing maize genetic variants is important for genome interpretation, population genetic analysis and functional analysis of allelic activity. We performed an isoform-level phasing study using two maize inbred lines and their reciprocal crosses, based on single-molecule, full-length cDNA sequencing. To phase and analyze transcripts between hybrids and parents, we developed IsoPhase. Using this tool, we validated the majority of SNPs called against matching short-read data from embryo, endosperm and root tissues, and identified allele-specific, gene-level and isoform-level differential expression between the inbred parental lines and hybrid offspring. After phasing 6907 genes in the reciprocal hybrids, we annotated the SNPs and identified large-effect genes. In addition, we identified parent-of-origin isoforms, distinct novel isoforms in maize parent and hybrid lines, and imprinted genes from different tissues. Finally, we characterized variation in cis- and trans-regulatory effects. Our study provides measures of haplotypic expression that could increase accuracy in studies of allelic expression.
Therapeutic strategy of arsenic trioxide in the fight against cancers and other diseases
Authors: Wang Qian Qian , Jiang Yu , Naranmandura Hua 
Published: February 2020

AbstractArsenic trioxide (ATO) has been recognized as a drug for the treatment of various diseases in traditional medicine for more than two thousand years. Although ATO has recently shown excellent efficacy for the treatment of acute promyelocytic leukemia (APL), it could not provide satisfactory outcomes as a single-agent for the management of non-APL leukemia or different solid tumors. Nevertheless, combination treatment strategies, e.g., ATO with other agents, have shown promising results against different diseases. Here, we introduce in depth the latest evidence and detailed insights into ATO-mediated cures for APL by targeting PML/RARα chimeric protein, followed by the preclinical and clinical efficacy of ATO on various non-APL malignancies and solid tumors. Likewise, the antiviral activity of ATO against human immunodeficiency virus (HIV) and hepatitis C virus (HCV) was also discussed briefly. Our review would provide a clear prospect for the combination of ATO with other agents for treatment of numerous neoplastic diseases, and open a new era in the clinically applicable range of arsenicals.
SKA3 promotes lung adenocarcinoma metastasis through the EGFR–PI3K–Akt axis
Authors: Hu Dan-dan , Chen Hai-ling , Lou Li-ming , Zhang Hong , Yang Guo-liang 
Published: February 2020

Abstract
The processes that lead to lung adenocarcinoma (LUAD) metastasis are poorly characterized. Spindle and kinetochore associated complex subunit 3 (SKA3) plays a key role in cervical cancer development, but its contribution to LUAD is unknown. Here, we found that SKA3 is overexpressed in LUAD and its expression correlates with lymph node metastasis and poor prognosis. SKA3 silencing experiments identified SKA3 as an oncogene that promotes the metastasis of LUAD cell lines and tissues. SKA3 was found to induce the expression of matrix metalloproteinase (MMP)-2, -7, and -9, which activate PI3K–AKT. SKA3 was also found to bind and activate EGFR to activate PI3K–AKT. In summary, we identify a role for SKA3 in LUAD metastasis through its ability to bind EFGR and activate PI3K–AKT signaling.
Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse
Authors: Weiss Alexander K.H. , Naschberger Andreas , Cappuccio Elia , Metzger Christina , Mottes Lorenza , Holzknecht Max , von Velsen Jill , Bowler Matthew W. , Rupp Bernhard , Jansen-Dürr Pidder 
Published: February 2020

Abstract
FAH domain containing protein 1 (FAHD1) is a mammalian mitochondrial protein, displaying bifunctionality as acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. We report the crystal structure of mouse FAHD1 and structural mapping of the active site of mouse FAHD1. Despite high structural similarity with human FAHD1, a rabbit monoclonal antibody (RabMab) could be produced that is able to recognize mouse FAHD1, but not the human form, whereas a polyclonal antibody recognized both proteins. Epitope mapping in combination with our deposited crystal structures revealed that the epitope overlaps with a reported SIRT3 deacetylation site in mouse FAHD1.
Preparations and characterisation of a nanosilver‐loaded aerogel based on nanocellulose
Authors: Zhang Lin , Li Qun , Duan Linjuan , Liu Rongrong 
Published: February 2020

Cellulose and nanocellulose were used as carriers to prepare composites with antimicrobial and catalytic properties. In this reported study, di‐aldehyde cellulose (DAC) was used as a raw material to prepare silver nanoparticles (AgNPs)/nano‐DAC (DANC) aerogels. The AgNPs/DANC aerogels were loaded with 24.78% nanosilver. The experimental materials were characterised using ultraviolet–visible, X‐ray diffraction, scanning electron microscopy, transmission electron microscopy, X‐ray photoelectron spectroscopy, and Brunauer–Emmett–Teller analysis. The test results showed that the AgNPs loaded on the DANC had particle sizes ranging from 3 to 14 nm, with an average particle size of 5.8 nm. The materials loaded onto the DANC were found to be elemental Ag. The specific surface area of AgNPs/DANC aerogels was 35.40 m2/g and the average pore diameter was 19.62 nm.
A Comparison of Matching and Weighting Methods for Causal Inference Based on Routine Health Insurance Data, or: What to do If an RCT is Impossible
Authors: Matschinger Herbert , Heider Dirk , König Hans-Helmut 
Published: February 2020

AbstractDue to a multitude of reasons Randomized Control Trials on the basis of so-called “routine data” provided by insurance companies cannot be conducted. Therefore the estimation of “causal effects” for any kind of treatment is hampered since systematic bias due to specific selection processes must be suspected. The basic problem of counterfactual, which is to evaluate the difference between two potential outcomes for the same unit, is discussed. The focus lies on the comparison of the performance of different approaches to control for systematic differences between treatment and control group. These strategies are all based on propensity scores, namely matching or pruning, IPTW (inverse probability treatment weighting) and entropy balancing. Methods to evaluate these strategies are presented. A logit model is employed with 87 predictors to estimate the propensity score or to estimate the entropy balancing weights. All analyses are restricted to estimate the ATT (Average Treatment Effect for the Treated) Exemplary data come from a prospective controlled intervention-study with two measurement occasions. Data contain 35 857 chronically ill insurants with diabetes, congestive heart failure, arteriosclerosis, coronary heart disease or hypertension of one German sickness fund. The intervention group was offered an individual telephone coaching to improve health behavior and slow down disease progression while the control group received treatment as usual. Randomization took place before the insurants’ consent to participate was obtained so assumptions of an RCT are violated. A weighted mixture model (difference-in-difference) as the causal model of interest is employed to estimate treatment effects in terms of costs distinguishing the categories outpatient costs, medication costs, and total costs. It is shown that entropy balancing performs best with respect to balancing treatment and control group at baseline for the first three moments of all 87 predictors. This will result in least biased estimates of the treatment effect.
Erhöhung der ventrikulären Reizschwelle durch Dronedaron-Einnahme
Authors: Wedler Katharina , Dávid Árpad , Körber Thomas 
Published: February 2020

Zusammenfassung
Anamnese Der 75-jährige Patient wurde mit Schwindel, Belastungsdyspnoe und Kaltschweißigkeit eingeliefert. Bei ischämischer Herzkrankheit mit Sick-Sinus-Syndrom erfolgte vor 5,5 Jahren die Schrittmacherimplantation. Aufgrund eines nicht permanenten Vorhofflimmerns wurde die Medikation vor 8 Monaten um Dronedaron erweitert.
Untersuchungen und Diagnose Im Status präsentierte sich ein instabiler Patient. Das 12-Kanal-EKG zeigte einen AV-Block 2. Grades mit Wenckebach-Periodik und einer Frequenz von 29/min. In der Schrittmacherabfrage fand sich primär bei regelrechter atrialer Funktion eine ineffektive ventrikuläre Stimulation.
Therapie und Verlauf Nach initial externer Stimulation erfolgte die Umprogrammierung des Schrittmachers und damit die effektive Ventrikelstimulation. Dronedaron wurde nach Prüfung auslösender Faktoren pausiert, und der Patient konnte nach 3 Tagen beschwerdefrei entlassen werden. In der Schrittmacherabfrage 18 Tage später fand sich eine regelrechte Funktion bei Stabilisierung der Reizschwelle nahe dem alten Niveau.
Folgerungen Bei Patienten mit permanentem Herzschrittmacher sollte als Ursache einer Fehlfunktion eine Reizschwellenerhöhung ausgeschlossen und bei Vorliegen die aktuelle Medikation hinterfragt werden. Dabei ist auch bei einer Dronedaron-Einnahme Vorsicht geboten.
Cross-Cultural Adaptation and Validation of the Arabic Version of the
Intermittent and Constant Osteoarthritis Pain Questionnaire
Authors: Alageel Musab , Al Turki Abdullah , Alhandi Ali , Alohali Rawa , Alsalem Rakan , Aleissa Sami 
Published: February 2020

AbstractThis study aimed to translate and adapt the Intermittent and Constant
Osteoarthritis Pain questionnaire into the Arabic language and evaluate the
validity and reliability of this scale for participants with knee or hip
osteoarthritis. This questionnaire was translated based on the Manufacturers
Alliance for Productivity and Innovation protocol. The test-retest reliability
was calculated using the Intraclass Correlation Coeﬃcient. Then,
Cronbach’s alpha was used to assess the internal consistency of
Intermittent and Constant Osteoarthritis Pain questionnaire. After that, the
criterion validity was evaluated against the Knee injury and Osteoarthritis
Outcome Score. A total of 90 participants were included in this study, of which
29 participants were re-evaluated for reliability testing. The Intraclass
Correlation Coeﬃcient of the Knee Intermittent and Constant
Osteoarthritis Pain questionnaire were 0.841, 0.923 and 0.911 for the total,
constant, and intermittent knee pain, respectively. Cronbach’s alpha was
0.88, 0.93 and 0.94 for the total score, the intermittent knee pain and the
constant knee pain, respectively. Eventually, criterion validity was
r=0.24(P<0.05). Intermittent and Constant Osteoarthritis Pain in
Arabic is a valid and reliable instrument to be used in Arabic-speaking patients
with knee/hip osteoarthritis.