bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictors of acute intracranial hemorrhage and recurrence of chronic subdural hematoma following burr hole drainage
Authors: Chen FuMei , Wang Ke , Xu KangLi , Wang Li , Zhan TianXiang , Cheng Fei , Wang Hao , Chen Zuo-Bing , Gao Liang , Yang Xiaofeng 
Published: February 2020

Abstract
Objective To investigate predictors of postoperative acute intracranial hemorrhage (AIH) and recurrence of chronic subdural hematoma (CSDH) after burr hole drainage. Methods A multicenter retrospective study of patients who underwent burr hole drainage for CSDH between January 2013 and March 2019. Results A total of 448 CSDH patients were enrolled in the study. CSDH recurrence occurred in 60 patients, with a recurrence rate of 13.4%. The mean time interval between initial burr hole drainage and recurrence was 40.8±28.3 days. Postoperative AIH developed in 23 patients, with an incidence of 5.1%. The mean time interval between initial burr hole drainage and postoperative AIH was 4.7±2.9 days. Bilateral hematoma, hyperdense hematoma and anticoagulant drug use were independent predictors of recurrence in the multiple logistic regression analyses. Preoperative headache was an independent risk factor of postoperative AIH in the multiple logistic regression analyses, however, intraoperative irrigation reduced the incidence of postoperative AIH. Conclusions This study found that bilateral hematoma, hyperdense hematoma and anticoagulant drug use were independently associated with CSDH recurrence. Clinical presentation of headache was the strongest predictor of postoperative AIH, and intraoperative irrigation decreased the incidence of postoperative AIH.
Impact of Surgeon Handedness in Manual and Robot-assisted Total Hip Arthroplasty
Authors: Kong Xiangpeng , Yang Minzhi , Li Xiang , Ni Ming , Zhang Guoqiang , Chen Jiying , Wei Chai 
Published: February 2020

Abstract
Background: The purpose of this study was to examine whether surgeon handedness could affect cup positioning in manual total hip arthroplasty (THA), and whether robot could diminish or eliminate the impact of surgeon handedness on cup positioning in robot-assisted THA.Methods: Fifty-three patients who underwent bilateral robot-assisted THA and sixty-two patients who underwent bilateral manual THA between August 2018 and July 2019 in our institute were respectively analyzed in this study. When the difference between the bilateral anteversion or inclination was greater than 5°, the patient was regarded as having different cup positioning between bilateral THA. Their demographics, orientation of acetabular cup and postoperative 3 month Harris hip score (HHS) were recorded for analysis. Results: There were no significant difference in the gender, age, BMI, diagnosis’s composition, preoperative and postoperative HHS between the robotic and manual group. Two left hips dislocated in the manual group. The anteversion of left hip was significantly larger than that of right hip (24.77±10.44 vs 22.44±8.67, p=0.043) in the manual group. There were no significant difference of cup positioning between bilateral robot-assisted THA. The patients in manual group were significantly more likely to have different cup positioning between bilateral hips than those in robotic group (77% vs 45%, p=0.000). More manual THA were located out of the target zone than robot-assisted THA (70% vs 48%, p=0.001). Conclusions: Surgeon’s handedness showed a trend towards an impact on cup positioning in manual THA and robot might help surgeon eliminate the adverse impact. However, the impact of handedness on the clinical outcomes still needs further observation.
Collagen Coating for Tissue Culture
Authors: McCullough Shaun D. 
Published: February 2020

Abstract
This protocol is intended for collagen coating of tissue culture dishes and Transwell® multiple well plates. The attached methods document is a formal version of the information included here.Disclaimer: The information presented here has been reviewed and approved for publication by the US Environ do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.
FITC-Dextran Trans-Epithelial Permeability Assay
Authors: Faber Samantha C. , McCullough Shaun D. 
Published: February 2020

Abstract
This protocol describes the use of the Fluorescein isothiocyanate (FITC)–dextran trans-epithelial permeability assay to quantify the effects of chemicals on epithelial cell monolayer permeability.The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.
Increasing access to CBT for psychosis patients: a feasibility, randomized controlled trial evaluating brief, targeted CBT for distressing voices delivered by Assistant Psychologists (GiVE2)
Authors: Hayward Mark , Berry Clio , Cameron Ben , Arnold Kate , Berry Katherine , Bremner Stephen , Cavanagh Kate , Fowler David , Gage Heather , Greenwood Kathryn , Hazell Cassie , Jones Anna-Marie , Robertson Sam , Strauss Clara 
Published: February 2020

Abstract
Background

The National Institute for Health and Care Excellence (NICE) recommends that Cognitive Behaviour Therapy for psychosis (CBTp) is offered to all patients with a psychosis diagnosis. However, only a minority of psychosis patients in England and Wales are offered CBTp. This is attributable, in part, to the resource-intensive nature of CBTp. One response to this problem has been the development of CBTp in brief formats that are targeted at a single symptom and the mechanisms that maintain distress. We have developed a brief form of CBTp for distressing voices and reported preliminary evidence for its effectiveness when delivered by highly trained therapists (Clinical Psychologists). This study will investigate the delivery of this intervention by a cost-effective workforce of Assistant Psychologists following a brief training and evaluate the acceptability and feasibility of conducting a future, definitive, randomised control trial (RCT).

Methods

This is a feasibility study for a pragmatic three-arm parallel group superiority 1:1:1 RCT comparing a Guided self-help CBT intervention for voices and treatment as usual (GiVE) to Supportive Counselling and treatment as usual (SC) to Treatment as usual alone (TAU), recruiting across two sites, with blinded post-treatment and follow-up assessments. A process evaluation will quantitatively and qualitatively explore stakeholder experience.

Discussion

Expected outcomes will include an assessment of the feasibility of conducting a definitive RCT and data to inform the calculation of its sample size. If evidence from a subsequent fully powered RCT suggests that GiVE is clinically and cost effective when delivered by briefly trained Assistant Psychologists, CBTp offered in these less resource-intensive forms has the potential to generate benefits for individual patients (reduced distress, enhanced recovery and enhanced quality of life), service-level patient benefit (increased access to evidence-based psychological therapies) and economic benefits to the NHS (in terms of the reduced use of mental health inpatient services).
Disclosure of HIV seropositivity to sexual partner and psychosocial factors in Ethiopia: Systematic review and meta-analysis&nbsp;
Authors: Endalamaw Aklilu , Geremew Demeke , Belete Habte , Dachew Berihun Assefa , Habtewold Tesfa Dejenie , Habtewold Tesfa Dejenie , Wilson Rhonda 
Published: February 2020

Abstract
Background: The burden of HIV/AIDS again becomes a public health problem after substantial control of its transmission in Ethiopia. For effective HIV transmission control measures, sero-disclosure to sexual partner is indispensable. Once the infection is established, psychosocial factors would have a great influence on HIV disclosure status to sexual partners which is very important to control viral transmission. This review aimed to estimate the national proportion of HIV disclosure practice to sexual partner and identify associated psychosocial factors.Methods: We searched PubMed, Scopus, African Journals Online, and Google Scholar databases. The Newcastle Ottawa quality assessment scale was used to assess the quality of studies. To ensure the absence or presence of publication bias, we used a funnel plot and performed Egger’s regression test for the subjective and objective assessment, respectively. Variation across studies was assessed using the I2 statistic. The pooled proportion was estimated by using weighted inverse variance random-effects model meta-analysis. We did subgroup and sensitivity analysis to explore the reason for heterogeneity and the impact of outlier finding on the overall estimation, respectively. Trend analysis was also performed to see the presence of time variation. Results: The proportion of HIV sero-disclosure practice to sexual partners was 76.03 % (95% Confidence Interval (CI): 68.78, 83.27). Being on ART (AOR=6.19; 95% CI: 2.92, 9.49), cohabiting with partner (AOR=4.48; 95% CI: 1.24, 7.72), getting counseling (AOR=3.94; 95% CI: 2.08, 5.80), had discussion prior to HIV testing (AOR= 4.40; 95% CI: 2.11, 6.69), awareness of partner’s HIV status (AOR= 6.08; 95%CI: 3.05, 9.10),smooth relationship with partner (AOR=4.44; 95% CI:1.28, 7.61), and being member of anti-HIV association (AOR=3.70; 95% CI: 2.20, 5.20) facilitates HIV status disclosure.Conclusions: In Ethiopia, still more than one-fourth of HIV-infected adults did not disclose their HIV positivity status to sexual partners. Psychosocial factors were the contributing factors of HIV-positive status disclosure. Further work is still needed to increase HIV status disclosure so as to decrease the transmission rate of HIV in Ethiopia.
Collaborative Model of Care between Orthopaedics and Allied Healthcare Professionals Trial (CONNACT) – A Feasibility Study in patients with knee osteoarthritis using a mixed method approach
Authors: Tan Bryan , DING Benjamin Tze Keong , PEREIRA Michelle Jessica , SKOU Soren Thorgaard , THUMBOO Julian , CAR Josip 
Published: February 2020

Abstract
Background Osteoarthritis is a leading cause of global disability resulting in significant morbidity and cost to the healthcare system. Current guidelines recommend lifestyle changes such exercises and weight loss as first line treatment prior to surgical consideration. Our current model of care is inefficient with suboptimal allied health intervention for effective behaviour changes. A 12-week community based, individualized, multidisciplinary new model of care for knee osteoarthritis was developed in light of current deficiencies. Methods The primary aim of this study was to determine the feasibility of a full randomized controlled trial evaluating this new model of care using pre-defined progression criteria. The secondary aim was to optimize the intervention and study design through a process evaluation. A pilot randomized trial design using a mixed method approach was utilized. Progression criteria for a full trial including key domains of patient recruitment and retention, outcome measure acceptability and improvement, adverse events were developed. The primary outcome measure was the Knee Injury and Osteoarthritis Outcome Score (KOOS) at baseline and 12-weeks. Secondary outcomes included quality of life, functional and psychological assessments. Semi-structured interviews were conducted with the patients at 12-weeks. Results 20 patients (3 males, 17 females) were randomized (10 intervention, 10 control). Intervention arm patients reported better improvements in their knee function, quality of life, psychological outcome, dietary improvement and weight loss compared to the control arm at 12-weeks. Semi-structured interviews revealed several themes pertaining to feasibility and intervention optimization. 5 out of the 6 progression criteria’s domains were met. Conclusion This pilot has demonstrated the feasibility of a full randomized control trial investigating the potential effectiveness of the new proposed model of care for knee osteoarthritis using pre-defined progression criteria and process evaluation. Results from the qualitative study were used to modify and improve the intervention content, delivery model and study design for a large effectiveness-implementation hybrid randomized control trial that is currently underway.
Down-regulation of Protein Tyrosine Phosphatase Receptor Gamma (PTPRG) in Chronic Myeloid Leukemia Patients in The State of Qatar is Due to Aberrant DNA Methylation Mechanism
Authors: Ismail Mohamed , Samara Muthanna , Sayab Ali Al , Alsharshani Mohamed , Yassin Mohamed , Vezzalini Marzia , Tomasello Luisa , Monne Maria , Morsi Hisham , Qoronfleh Walid , Zaid Hatem , Cook Richard , Sorio Claudio , Modjtahedi Helmout , Al-Dewik Nader 
Published: February 2020

Abstract
Background: Several studies showed that the aberrant DNA methylations are involved in leukemia and cancer pathogenesis. PTPRG is a natural inhibitory mechanism that is found to be down-regulated in Chronic Myeloid Leukemia (CML) disease. The mechanism behind its down-regulation has not been fully elucidated. Aim: The study investigates the role of methylation as a possible mechanism of PTPRG under-expression in CML patients. Method: Peripheral blood samples from CML patients treated with tyrosine kinase inhibitors (TKIs) and healthy controls were collected. DNA was extracted and treated with bisulfite treatment, followed by PCR and sequencing of 25 of CpG in Promoter and 26 of CpG in the Intronic regions of PTPRG. Bisulfite-sequencing technique as a high-resolution method was employed. Results : CML groups (New Diagnosed and Failed treatment) have significantly higher methylation levels in the Promoter and Intron regions compared to the healthy group. There were also significant differences in methylation levels of CpG sites in the Promoter and Intronic regions amongst the groups. Conclusion: Aberrant methylation of PTPRG is documented and potentially is one of the possible mechanisms of PTPRG down-regulation in CML.
Prevalence and associated factors of anemia among children aged 6-59 months in Ethiopia: Evidence from the Ethiopia Demographic and Health Survey
Authors: Woya Ashenafi Abate , Tekile Abay Kassa 
Published: February 2020

Abstract
Anemia is one of the most widely spread public health problems, especially in developing countries including Ethiopia. Thus, the aim of this study was to assess the prevalence and associated factors of anemia among children aged 6-59 months in Ethiopia. This study utilized secondary data from the 2016 Ethiopia Demographic and Health Survey. The data were analyzed using by STATA software version 14. Ordinal logistic regression statistical analysis was used to identify risk factors of anemia. Out of 8385 children considered in this study, more than half (57.3%) of them were anemic: 262(3.1%) were severely anemic, 2447(29.2%) were moderately anemic, and 2100(25.0%) were mildly anemic. Mothers’ anemic status and children anemic status were highly associated. Accordingly, among severely anemic mothers, 26.8% of children were severely anemic, among moderately anemic mothers, 39.7% of children were moderately anemic. Mother’s anemic status, place of residence, child age, smoking status, religion, and region of residence were factors associated with a prevalence of childhood anemia. A of anemia among children aged 6-59 months in Ethiopia was a severe public health problem. Thus, creating awareness among mothers how to control and manage the anemia status of their children and Routine iron supplementation are recommended to reduce childhood anemia.
Clinical Outcomes and Factors of Single Attempt of Stent Retrieval during Endovascular Thrombectomy for Acute Ischemic Stroke
Authors: Liu Mingli , Liu Yang , Lin Lin , Li Yongli , Yan Feng , Wang Rui , Cai Jinquan , Li Ruiyan 
Published: February 2020

Abstract
Backgrounds: Single attempt of stent retrieval during endovascular thrombectomy (EVT) seems to be associated with favorable clinical outcomes of patients with acute ischemic stroke (AIS), but the relationship between single attempt and clinical outcomes or complications have not been characterized. In addition, the factors related to single retrieval attempts during EVT are undefined. Here, we investigated the relationship between single attempt of stent retrieval and the clinical outcomes or complications after EVT, and further investigated the factors associated with single attempt of stent retrieval in AIS patients.
Methods: This study enrolled consecutive patients with AIS treated by EVT with second-generation stent retriever devices from April 2016 to April 2019. Attempts of EVT were classified as single or multiple attempts of stent retrieval after filtrating all enrolled patients based on inclusion and exclusion criteria. We analyzed the clinical outcomes and complications between single attempt of stent retrieval and multiple attempts of stent retrieval, and the factors independently associated with a single attempt were identified through logistical regression analysis.
Results: We enrolled 143 patients, including 69 patients with single attempt of stent retrieval and 74 patients with multiple attempts of stent retrieval. More patients with single attempt of stent retrieval reached favorable clinical outcomes ( p =0.016; adjusted OR [95%CI]: 2.652 [1.204-5.843] ), and patients with single attempts of stent retrieval showed as a lower incidence of symptomatic intracranial hemorrhage (sICH) ( p =0.046; adjusted OR [95%CI]: 0.192 [0.038-0.973] ). Diabetes mellitus ( p =0.026; adjusted OR [95%CI]: 2.871 [1.137-7.249] ) was independently associated with single attempt of EVT with stent retrieval.
Conclusions: Single attempt of stent retrieval during EVT can decrease incidence of sICH and improve favorable clinical outcomes of patients with AIS. Those with diabetes mellitus may be more prone to single attempt of stent retrieval during EVT .
Unravelling the role of Mesenchymal Stem/Stromal Cells Secretome in intervertebral disc degeneration in a pro-inflammatory/degenerative ex vivo model
Authors: Ferreira JR , Teixeira GQ , Neto E , Ribeiro-Machado C , Silva AM , Caldeira J , Pereira C Leite , Bidarra S , Maia AF , Lamghari M , Barbosa MA , Gonçalves RM 
Published: February 2020

Abstract
Background: Mesenchymal stem/stromal cells (MSCs) have been increasingly used in clinical trials for intervertebral disc (IVD) degeneration. Here, we aimed to evaluate the potential of a cell-free approach to degenerated IVD, testing if MSCs secretome can stimulate a regenerative response by modulating the IVD inflammatory cascade.
Methods: Human bone marrow-derived MSCs were pre-conditioned with IL-1β (10 ng/mL) and low oxygen (6% O2). The secretome of MSCs (MSCsec) was collected after 48h. Bovine IVD tissue explants cultured in pro-inflammatory/degenerative conditions (needle puncture + IL-1β) were treated with MSCsec or co-cultured with MSCs.
Results: MSCsec obtained upon IL-1β-pre-conditioning, as well as MSCs co-culture, down-regulated gene expression of pro-inflammatory cytokines, bIL-6 and bIL-8 after 48h, in IVD. IVD matrix degrading enzymes, bMMP1 and bMMP3, were downregulated and upregulated, respectively, in the presence of MSCsec, but not MSCs. After 14 days, MSCsec-treated IVDs revealed increased aggrecan content at the protein level, contrarily to MSCs/IVD co-cultures. Interestingly, IL-1β-preconditioning only, but not IL-1β-IVD, increased gene expression of hADAMTS5 and hTIMP-1in MSCs. Additionally, conditioned medium from MSCsec-treated IVDs did not promote angiogenesis or neurogenesis. In MSCsec-treated IVD, an increase in MCP-3 and GCP-2 was observed, while SDF-1α, TNF-α, IGF-1, Eotaxin 3, FGF-9, MIP-1δ, IFN-γ, IL-5, TNF-β, IL-4, TGF-β1, IL-16, IGFBP-3 and IGFBP-4 were decreased, compared with MSCs/IVD co-cultures.
Conclusions: MSCsec obtained upon IL1β-preconditioning, present an immunomodulatory role in degenerated IVD, as well as MSCs. Nevertheless, MSCsec but not MSCs, potentiate aggrecan deposition in IVD in pro-inflammatory/degenerative conditions. This finding can open new perspective on the use of MSCsec as a cell-based/cell-free approach to LBP.
A retrospective study of lumbar spine fusion surgery with the Wiltse approach and the traditional approach
Authors: Cheng Weiyi , Zeng Xixi , Gui Tong 
Published: February 2020

Abstract
Background： This study aimed to compare the clinical results between the Wiltse approach and traditional approach in lumbar spine fusion. Methods: In this retrospective controlled study, from May 2016 to May 2017, 70 cases with lumbar spine fusion surgery for lumbar disc herniation or lumbar spondylolisthesis within Ⅱdegree were assigned to Wiltse approach (35 cases,18 males and 17 females; mean age 52 years) and traditional approach groups (35cases, 19 males, 16 females; 51 years). There were totally 38 cases of lumbar disc herniation and 32 cases of II- degree spondylolisthesis in two groups. The operation time, the amount of blood loss and post-operation drainage, the level of creatine phosphokinase (CK) and the reduction of cross sectional area (CSA) in magnetic resonance imaging (MRI) were recorded. Results: After the operation for the Wiltse approach group, there was less operation time, blood loss and post-operative drainage (p＜0.05) ;the peripheral blood CK levels was changed a lot in 1 day and 3 days after the operation. In the Wiltse group, the reduction of CSA was significantly lower than traditional approach group in MRI. Conclusion: For lumbar spinal fusion surgery, the Wiltse approach do have shorter operation time and smaller vertebral side muscle injury compared with the traditional approach. However, in determining the operation program, the performer should fully recognize that the Wiltse clearance may have different influence on the operation at different anatomical level.