bluuebunny/crossref_metadata_2025_split huggingface dataset:
Political budget cycles and voting within a federal country: The influence of political alignment
Authors: Garofalo Pablo , Lema Daniel , Streb Jorge M. 
Published: February 2020

AbstractWe study how distributive politics affects political budget cycles and voting within a federal country. Our model predicts not only that the president favors politically aligned governors with larger transfers, but also that voters favor gubernatorial candidates aligned with the expected presidential incumbent because larger transfers are expected in the future. These predictions are upheld by the data from Argentinean provinces during the 1984–2014 period. Our findings imply that political alignment can trump the selection effect of voting the most competent candidate. This can help explain in particular coattail effects when gubernatorial and presidential elections are concurrent.
Stable isotopes reveal niche segregation between native and non‐native Hoplias in a Neotropical floodplain
Authors: Alves Gustavo H. Zaia , Cionek Vivian M. , Manetta Gislaine I. , Pazianoto Laryssa H. R. , Benedito Evanilde 
Published: February 2020

AbstractWe investigated whether isotopic niche partitioning could mediate the coexistence between native (Hoplias sp. B) and non‐native (Hoplias mbigua) trahira in a Neotropical floodplain. We hypothesised that during the dry season both species inhabit isolated sites (i.e. lakes) and thus rely on distinct food resources to sustain their coexistence. We found evidence of trophic segregation among native and non‐native freshwater fish in the Upper Paraná River, Brazil. Even though both species rely on the same primary littoral carbon sources and present similar trophic diversity, trophic segregation was attained through larger niche amplitude and smaller isotopic niche width for the non‐native fish. Three decades after initial invasion, non‐native Hoplias have distinct foraging behaviour and do not compete for trophic resources with native trahira; this is likely due to morpho‐anatomical differences. Limited morphological similarity between these congeneric species might be the strongest factor contributing to their coexistence in the Paraná River floodplain during the dry season.
EGFL6 expression in hair follicle central isthmus is dependent on the presence of terminal Schwann cells
Authors: Wei Jiang‐Ping , Gong Yao , Zhong Hong‐Bing , Hua Wang Tim , Liao Xin‐Hua 
Published: February 2020

AbstractHair follicle central isthmus is surrounded by dense nerve endings and terminal Schwann cells (TSCs), forming a specialized sensory structure called lanceolate complexes. Extracellular matrix protein EGFL6 expressed from epidermis has been found closely associated with lanceolate complexes and important for proper alignment of nerve fibres and TSCs processes, and for proper response to light touch. However, how EGFL6 itself is specifically induced/deposited/maintained at the central isthmus remains to be elucidated. Previous reports and our results showed that nerve endings and TSCs docking at the central isthmus during hair follicle development occur before the specific depositing of EGFL6 protein. Furthermore, we found nude mice rarely maintain the lanceolate complex, and EGFL6 is lost in their aberrant hair follicle. Instead, reconstituted hair follicle in nude mice by stem cells chamber grafting assay expresses EGFL6 at the central isthmus area after hair follicle innervation. At last, long‐term but not short‐term cutaneous denervation leads to degeneration of TSCs and loss of EGFL6 expression. Together, our results demonstrate that EGFL6 expression in the central isthmus is dependent on the presence of TSCs, proposing that the interplay of epidermis and neuronal components is important for maintaining functional structure of lanceolate complexes.
Optimization of TSV interconnects and BEOL layers under annealing process through fracture evaluation
Authors: Qin F. , Zhang M. , Dai Y. , Chen P. , An T. , He H. , Zhang H. , Zheng J. 
Published: February 2020

AbstractThrough silicon via (TSV) is a crucial interconnection structure in 3‐D integrated circuits. However, protrusion and intrusion of TSV‐Cu caused by annealing could lead to cracking and failure of back‐end‐of‐line (BEOL) layers and TSV interconnects due to mismatch of coefficient of thermal expansion. In this paper, optimizations of TSV interconnects and BEOL layers under annealing process are investigated based on fracture evaluation. Influences of geometrical factors including the TSV geometry dimension, the distance between TSV and BEOL layers, and pitch size of Cu via on energy release rate and J‐integral are studied for TSV interconnects and BEOL layers with cracks. Effect of material properties for low k dielectrics on interfacial fracture of BEOL layers and TSV interconnects is also given. Optimized geometrical factors and optimized material properties of low k dielectrics are presented in this paper. Fracture‐based method sheds a light on emerging electronic packaging optimization.
Projecting global mariculture diversity under climate change
Authors: Oyinlola Muhammed A. , Reygondeau Gabriel , Wabnitz Colette C. C. , Cheung William W. L. 
Published: February 2020

AbstractPrevious studies have focused on changes in the geographical distribution of terrestrial biomes and species targeted by marine capture fisheries due to climate change impacts. Given mariculture's substantial contribution to global seafood production and its growing significance in recent decades, it is essential to evaluate the effects of climate change on mariculture and their socio‐economic consequences. Here, we projected climate change impacts on the marine aquaculture diversity for 85 of the currently most commonly farmed fish and invertebrate species in the world's coastal and/or open ocean areas. Results of ensemble projections from three Earth system models and three species distribution models show that climate change may lead to a substantial redistribution of mariculture species richness potential, with an average of 10%–40% decline in the number of species being potentially suitable to be farmed in tropical to subtropical regions. In contrast, mariculture species richness potential is projected to increase by about 40% at higher latitudes under the ‘no mitigation policy’ scenario (RCP 8.5) by the mid‐21st century. In Exclusive Economic Zones where mariculture is currently undertaken, we projected an average future decline of 1.3% and 5% in mariculture species richness potential under RCP 2.6 (‘strong mitigation’) and RCP 8.5 scenarios, respectively, by the 2050s relative to the 2000s. Our findings highlight the opportunities and challenges for climate adaptation in the mariculture sector through the redistribution of farmed species and expansion of mariculture locations. Our results can help inform adaptation planning and governance mechanisms to minimize local environmental impacts and potential conflicts with other marine and coastal sectors in the future.
LINC02499, a novel liver‐specific long non‐coding RNA with potential diagnostic and prognostic value, inhibits hepatocellular carcinoma cell proliferation, migration, and invasion
Authors: Ma Xiaoyun , Mo Meile , Tan Hui Juan Jennifer , Tan Chao , Zeng Xiaoyun , Zhang Guoqiang , Huang Dongping , Liang Jun , Liu Shun , Qiu Xiaoqiang 
Published: February 2020

AimLiver‐specific non‐coding RNAs have been reported to play crucial roles in hepatocellular carcinoma (HCC). We investigated the possible biological performance of a novel liver‐specific long non‐coding RNA, LINC02499, in HCC.MethodsThe association between LINC02499 expression and HCC was evaluated based on The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, and then confirmed in a HCC cohort by quantitative real‐time polymerase chain reaction. The effects of LINC02499 on HCC cells were verified by gain‐ and loss‐of‐function assays. Pathway enrichment analyses were used to explore the potential mechanism of LINC02499 in HCC.ResultsLINC02499 expression was remarkably decreased in HCC tissues compared to adjacent non‐tumor tissues based on TCGA (P < 0.001) and GEO databases (P < 0.001) and our HCC cohort (P < 0.001). Decreased LINC02499 was also significantly associated with poorer overall survival in both the TCGA database (P = 0.009) and our HCC cohort (P = 0.002). Furthermore, the receiver operating characteristic analysis indicated that LINC02499 showed a good performance in HCC diagnosis (area under the curve = 0.879, P < 0.001), and both sensitivity and specificity were 83.8%. In addition, up‐ and downregulated LINC02499 significantly impacted proliferation, migration, and invasion abilities of HCC cells in vitro. Pathway enrichment analyses revealed that the potential target genes of LINC02499 were involved in “Complement and coagulation cascades” and “Butanoate metabolism” pathways.ConclusionLINC02499 could be a potential novel diagnostic and prognostic biomarker for HCC patients, and it could exert a tumor suppressor role in the progression of HCC.
Effects of two yellowing process on colour, taste and nonvolatile compounds of bud yellow tea
Authors: Wei Yuming , Fang Shimao , Jin Ge , Ni Tiancheng , Hou Zhiwei , Li Tiehan , Deng Wei‐Wei , Ning Jingming 
Published: February 2020

AbstractThe current method for processing bud yellow tea usually results in astringent taste that is not sufficiently yellowing. Therefore, two factors in the production phase that affect tea quality were studied: number of times the tea leaves undergo the yellowing process and predrying of leaves to reduce their water content before a second yellowing process. We then evaluated the colour and nonvolatile compounds of the produced tea. Results revealed that gallated catechins and the polyphenol–amino acid ratio decreased significantly, and the colour of the tea changed from green to yellow after two yellowing processes. Predrying leaves to a lower water content resulted in the accumulation of free amino acids and soluble sugars, and an increase in the brightness and yellow colour of tea infusion. Bud yellow tea with the highest taste evaluation score and yellowness was obtained when the leave samples underwent two yellowing processes and the water content in the leaves was 40%.
DOAC plasma levels measured by chromogenic anti‐Xa assays and HPLC‐UV in apixaban‐ and rivaroxaban‐treated patients from the START‐Register
Authors: Cini Michela , Legnani Cristina , Padrini Roberto , Cosmi Benilde , Dellanoce Claudia , De Rosa Giovanni , Marcucci Rossella , Pengo Vittorio , Poli Daniela , Testa Sophie , Palareti Gualtiero 
Published: February 2020

AbstractIntroductionTo measure direct factor Xa inhibitor (apixaban, edoxaban, rivaroxaban) concentrations, dedicated chromogenic anti‐Xa assays are recommended as suitable methods to provide rapid drug quantification. Moreover, the high‐performance liquid chromatography with ultraviolet detection (HPLC‐UV) is reported as a reliable quantitative technique. We investigated seven anti‐Xa assays and an HPLC‐UV method for measurement of apixaban and rivaroxaban levels in patients enrolled in the START‐Register.MethodsA total of 127 apixaban and 124 rivaroxaban samples were tested by HPLC‐UV and the following anti‐Xa assays: Biophen DiXaI and Heparin LRT (Hyphen BioMed), Berichrom and Innovance Heparin (Siemens), STA‐Liquid Anti‐Xa (Stago Diagnostics), Technochrom anti‐Xa (Technoclone), and HemosIL Liquid Anti‐Xa (Werfen). Each method was performed in one of the participating laboratories: Bologna, Cremona, Florence, and Padua.ResultsOur data confirmed the overestimation of apixaban and rivaroxaban levels by the antithrombin‐supplemented anti‐Xa method (Berichrom). Performances and reproducibility of the six anti‐Xa assays not supplemented with antithrombin and the HPLC‐UV method were good, with limits of quantification from 8‐39 ng/mL (apixaban) and 15‐33 ng/mL (rivaroxaban). The six chromogenic methods showed good concordances with the quantitative HPLC‐UV [bias: −26.9‐22.3 ng/mL (apixaban), −11.3‐18.7 ng/mL (rivaroxaban)]. Higher bias and wider range between limits of agreement were observed at higher concentrations [<100 ng/mL: bias −21.3‐4.1 ng/mL (apixaban) and −6.2‐3.8 ng/mL (rivaroxaban); >200 ng/mL: bias −42.2‐36.8 ng/mL (apixaban) and −20.1‐68.9 ng/mL (rivaroxaban)].ConclusionOverall, the anti‐Xa assays not supplemented with antithrombin and the HPLC‐UV method proved to be suitable for apixaban and rivaroxaban quantification.
Being an internist throughout the world – same name, different training curricula
Authors: Freire Mayka , Sopeña Bernardo , Carballo Iago , Diaz‐Peromingo José A. , Vazquez‐Agra Nestor , Gonzalez‐Quintela Arturo 
Published: February 2020

AbstractIn the 79 countries revised, two fundamental internal medicine (IM) training patterns were in evidence, first, only basic training needed, requiring a 3–4‐year residency period for obtaining the diploma of internist; and advanced training needed, requiring 5–7 years of study. Second, evaluation of common IM training for sub‐specialists revealed the following three patterns: dual training; core training and separate training.
Antithrombin III as predictive indicator of survival in idiopathic pulmonary fibrosis (IPF) patients treated with nintedanib: a preliminary study
Authors: Bergantini Laura , d'Alessandro Miriana , Cameli Paolo , Carleo Alfonso , Landi Claudia , Vietri Lucia , Lanzarone Nicola , Pieroni Maria , Sestini Piersante , Bargagli Elena 
Published: February 2020

AbstractBackgroundIdiopathic pulmonary fibrosis (IPF) is a progressive lung disease often managed with nintedanib, a tyrosine kinase inhibitor targeting several profibrotic pathways. Although clotting processes are involved in wound healing and repair in the lung, there are no data on the role of antithrombin III (ATIII) in IPF patients treated with nintedanib. A previous proteomic analysis of serum of IPF patients before and after 1 year of nintedanib treatment showed differential protein expression of ATIII.AimsHere we used quantitative methods to evaluate differential ATIII concentrations in IPF patients before and after 1 year of nintedanib treatment and to assess the potential of ATIII as a prognostic biomarker in IPF patients.MethodsSerum levels of ATIII were measured by enzyme‐linked immunosorbent assay in 14 IPF patients before and after 1 year of nintedanib treatment.ResultsA statistically significant inverse correlation was found between serum ATIII concentrations and pulmonary function test parameters in all patients at baseline and follow up. Baseline serum ATIII and bronchoalveolar lavage (BAL) neutrophils proved to be reliable predictors of poor prognosis. A baseline ATIII threshold of 126.5 μg/mL discriminated survivors from non‐survivors.ConclusionsAfter 12 months of antifibrotic treatment, IPF patients with high serum ATIII concentrations and high BAL neutrophil percentages had a poor prognosis and increased survival risk. The results of this preliminary study suggest that ATIII has potential as a biomarker of IPF severity and in predicting response to nintedanib therapy. As a marker, ATIII showed several advantages over BAL neutrophil percentage.
Post‐secondary students with symptoms of psychosis: A mixed‐methods systematic review
Authors: Sanderson Victoria A. , Vandyk Amanda Digel , Graham Ian D. , Lightfoot Sophie , Murawsky Mackenzie , Sikora Lindsey , Jacob Jean Daniel 
Published: February 2020

AbstractPost‐secondary students are at a heightened risk of psychosis, yet little is known about this population. The aim of this study was to conduct a mixed‐methods systematic review of psychosis among post‐secondary students, including student characteristics, overall prevalence, risk factors, interventions, and their reported experiences. Procedures were modelled on the Joanna Briggs Institute approach, while PRISMA guided conduct and reporting. A librarian created a systematic search of nine databases, and quality was assessed using JBI Critical Appraisal Tools. We pooled and analysed data according to objectives and recorded data using synthesis tables and narrative summaries. 26 articles published between 2006 and 2018 are included. The average age of participants was 21 years, and most identified as female and Caucasian. The prevalence of psychosis was often measured in a way that limited comparison across studies. Risk factors associated with symptoms of psychosis included substance use, depression, and younger age. Other risk factors trending towards a relationship included racial discrimination and anxiety. Protective factors included self‐esteem and self‐concept clarity. Five interventions were studied, with mixed results, and only three studies reported on the experience of students. While five promising interventions are identified, inadequate testing and replication limit confidence in their effectiveness and there is a notable deficit in qualitative evidence exploring the experiences of these students. Integrating knowledge on risk factors, interventions, and experiences of students with psychosis will help tailor and facilitate their health care while protecting their right to education.
Hydration and reaction mechanisms on sodium silicate glass surfaces from molecular dynamics simulations with reactive force fields
Authors: Mahadevan Thiruvilla S. , Du Jincheng 
Published: February 2020

AbstractRecent development of reactive force fields have enabled molecular dynamics simulations of interactions between silicate glasses and water at the atomistic scale. While multicomponent silicate glasses encompass a wide variety of compositions and properties, one common structural feature in these glasses is the combination of the network structure that is made up of silica tetrahedra linked through corner sharing interspersed with network modifiers like alkali and alkaline‐earth ions that break up the Si–O–Si linkages by forming nonbridging oxygen. In reactions with water, ion exchange between alkali ions in the glass and proton or hydronium in the solution, as well as hydrolysis reaction of the Si–O–Si linkages and subsequent silanol formation, is observed and well documented. We have used a set of recently developed reactive force field to investigate the reactions between water and the surfaces of silica and sodium silicate glasses of different compositions for reactions up to 8 nanoseconds. Our results indicate sodium leaching into water and diffusion of water molecules up to 25 Å into the glass surface. We examined the structural and compositional changes inside the glass and around the diffused ions and use these to explain the rates of silanol formation at the surface. We also observed proton transport in the glass which has an indirect influence on the silanol formation rates. While the surface of the glass was rough to start with, it undergoes further modification into a hydrated gel‐like structure in the glass for up to 5 Å in the higher alkali containing glasses. It was found that the leached sodium ions remain close to the interface and that fragments of silicate network from the surface is capable of dislodging from the bulk glass and enter the aqueous solution. These simulations thus provide insights into the formation and structure of an alteration layers commonly observed in multicomponent silicate glasses corroded in aqueous solutions.
The rapidly advancing Class 2 CRISPR‐Cas technologies: A customizable toolbox for molecular manipulations
Authors: Wang Jingyi , Zhang Chenzi , Feng Bo 
Published: February 2020

AbstractThe CRISPR‐Cas technologies derived from bacterial and archaeal adaptive immune systems have emerged as a series of groundbreaking nucleic acid‐guided gene editing tools, ultimately standing out among several engineered nucleases because of their high efficiency, sequence‐specific targeting, ease of programming and versatility. Facilitated by the advancement across multiple disciplines such as bioinformatics, structural biology and high‐throughput sequencing, the discoveries and engineering of various innovative CRISPR‐Cas systems are rapidly expanding the CRISPR toolbox. This is revolutionizing not only genome editing but also various other types of nucleic acid‐guided manipulations such as transcriptional control and genomic imaging. Meanwhile, the adaptation of various CRISPR strategies in multiple settings has realized numerous previously non‐existing applications, ranging from the introduction of sophisticated approaches in basic research to impactful agricultural and therapeutic applications. Here, we summarize the recent advances of CRISPR technologies and strategies, as well as their impactful applications.
Methyltransferase like 3 promotes colorectal cancer proliferation by stabilizing CCNE1 mRNA in an m6A‐dependent manner
Authors: Zhu Wei , Si Yan , Xu Jun , Lin Yu , Wang Jing‐Zi , Cao Mengda , Sun Shanwen , Ding Qiang , Zhu Lingjun , Wei Ji‐Fu 
Published: February 2020

Abstractm6A modification is the most prevalent RNA modification in eukaryotes. As the critical N6‐methyladenosine (m6A) methyltransferase, the roles of methyltransferase like 3 (METTL3) in colorectal cancer (CRC) are controversial. Here, we confirmed that METTL3, a critical m6A methyltransferase, could facilitate CRC progression in vitro and in vivo. Further, we found METTL3 promoted CRC cell proliferation by methylating the m6A site in 3′‐untranslated region (UTR) of CCNE1 mRNA to stabilize it. Moreover, we found butyrate, a classical intestinal microbial metabolite, could down‐regulate the expression of METTL3 and related cyclin E1 to inhibit CRC development. METTL3 promotes CRC proliferation by stabilizing CCNE1 mRNA in an m6A‐dependent manner, representing a promising therapeutic strategy for the treatment of CRC.
Thioredoxin‐interacting protein promotes activation and inflammation of monocytes with DNA demethylation in coronary artery disease
Authors: Rong Jialing , Xu Xianqun , Xiang Yang , Yang Guohua , Ming Xinliang , He Siying , Liang Bin , Zhang Xiaokang , Zheng Fang 
Published: February 2020

AbstractNumerous studies have demonstrated that thioredoxin‐interacting protein (TXNIP) expression of peripheral blood leucocytes is increased in coronary artery disease (CAD). However, the molecular mechanism of this phenomenon remained unclear. DNA methylation plays important roles in the regulation of gene expression. Therefore, we speculated there might be a close association between the expression of TXNIP and methylation. In this study, we found that compared with controls, DNA methylation at cg19693031 was decreased in CAD, while mRNA expressions of TXNIP and inflammatory factors, NLRP3, IL‐1β, IL‐18, were increased. Methylation at cg19693031 was negatively associated with TXNIP expression in the cohort, THP‐1 and macrophages/foam cells. Furthermore, Transwell assay and co‐cultured adhesion assay were performed to investigate functions of TXNIP on the migration of THP‐1 or the adhesion of THP‐1 on the surface of endothelial cells, respectively. Notably, overexpressed TXNIP promoted the migration and adhesion of THP‐1 cells and expressions of NLRP3, IL‐18 and IL‐1β. Oppositely, knock‐down TXNIP inhibited the migration and adhesion of THP‐1 and expressions of NLRP3, IL‐18. In conclusion, increased TXNIP expression, related to cg19693031 demethylation orientates monocytes towards an inflammatory status through the NLRP3 inflammasome pathway involved in the development of CAD.