bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estudo da política de educação profissional na Bahia orientada para a inclusão social
Authors: Silva Rosemary Lopes Soares da 
Published: April 2020

Explicitar as técnicas e procedimentos, os conceitos e categorias com as quais a interpretação dos documentos relativos a Política da Educação Profissional Técnica de Nível Médio –, implementada na Bahia / Brasil, especificamente, a partir da abordagem quadripolar que conferiu a densidade teórico-metodológica para o estudo do objeto na relação com os polos epistemológicos, teóricos, técnicos e morfológicos. Nesta perspectiva, a realização de uma pesquisa não é o cumprimento de “ritualismo metodológico” com um “modismo teórico” para não repetir o que comumente acontece nas pesquisas em educação, de maneira progressivamente mais intensa, de tentativas ecléticas que juntam aleatoriamente técnicas, métodos e referenciais teóricos sem uma clara compreensão dos fundamentos epistemológicos dos diversos caminhos do conhecimento.
The Urgency of Guidelines for the Long-term Sustainability of Outer Space Activities for Indonesia
Authors: Permatasari Yunita 
Published: April 2020

The rapid increase in the use and exploration of outer space raises concerns about its sustainability in a sustainable manner. Countries seek multilateral solutions through the guidelines for the long-term sustainability of outer space activities (LTSOSA) which was adopted in June 2019 in the United Nations Committee on the Peaceful Uses of Outer Space (UNCOPUOS) forum. Indonesia as a country that organizes the use and exploration of outer space has an interest in ensuring the security of the implementation of outer space in a sustainable manner. Thus, it is necessary for the mapping of Indonesia’s interest in implementing the LTSOSA guidelines. The interest mapping will illustrate the urgency of the LTSOSA guidelines for Indonesia's national security particularly outer space security. The analysis shows that the LTSOSA guidelines contain items related to outer space security which are of vital national importance to Indonesia. This article is expected to become input for Indonesia's attitude and position towards the implementation of the LTSOSA guidelines.Keywords: Indonesia, long term sustainability of outer space activities, outer space security Penggunaan dan eksplorasi antariksa yang semakin pesat menimbulkan kekhawatiran terhadap kelestariannya secara berkelanjutan. Negara-negara mencari solusi secara multilateral melalui pedoman kegiatan antariksa berkelanjutan jangka panjang atau dikenal guidelines for the long-term sustainability of outer outer space activities (LTSOSA) yang disahkan pada Juni 2019 dalam forum United Nations Committee on the Peaceful Uses of Outer Outer space (UNCOPUOS). Indonesia sebagai negara yang menyelenggarakan penggunaan dan eksplorasi antariksa berkepentingan untuk menjamin keamanan penyelenggaraan keantariksaannya secara berkelanjutan. Dengan demikian, perlu adanya pemetaan kepentingan Indonesia dalam mengimplementasikan guidelines LTSOSA. Pemetaan kepentingan akan menggambarkan mengenai urgensi guidelines LTSOSA bagi keamanan nasional Indonesia khususnya bidang keantariksaan. Tulisan ini menemukan bahwa guidelines LTSOSA memuat butir-butir terkait keamanan keantariksaan yang menjadi kepentingan nasional vital bagi Indonesia. Artikel ini diharapkan menjadi bahan masukan sikap dan posisi Indonesia terhadap implementasi guidelines LTSOSA. Kata-kata kunci: Indonesia, keamanan keantariksaan, keberlanjutan kegiatan luar angkasa dalam jangka panjang
Intra-specific comparison of mitochondrial genomes reveals host gene fragment exchange via intron mobility in Tremella fuciformis
Authors: Deng Youjin , Zhang Xunxiao , Xie Baogui , Lin Longji , Hsiang Tom , Lin Xiangzhi , Lin Yiying , Zhang Xingtan , Ma Yanhong , Ming Ray , Miao Wenjing 
Published: April 2020

Abstract
Background Mitochondrial genomic sequences are known to be variable. Comparative analyses of mitochondrial genomes can reveal the nature and extent of their variation. Results Draft mitochondrial genomes of 16 Tremella fuciformis isolates (TF01-TF16) were assembled from Illumina and PacBio sequencing data. Mitochondrial DNA contigs were extracted and assembled into complete circular molecules, ranging from 35,104 bp to 49,044 bp in size. All mtDNAs contained the same set of 41 conserved genes with identical gene order. Comparative analyses revealed that introns and intergenic regions were variable, whereas genic regions (including coding sequences, tRNA, and rRNA genes) were conserved. Among 24 introns detected, 11 were in protein-coding genes, 3 in tRNA genes, and the other 10 in rRNA genes. In addition, two mobile fragments were found in intergenic regions. Interestingly, six introns containing N-terminal duplication of the host genes were found in five conserved protein-coding gene sequences. Comparison of genes with and without these introns gave rise to the following proposed model: gene fragment exchange with other species can occur via gain or loss of introns with N-terminal duplication of the host genes. Conclusions Our findings suggest a novel mechanism of fungal mitochondrial gene evolution: partial foreign gene replacement though intron mobility.
Sensor-based Characterization of Daily Walking: A New Paradigm in Pre-frailty/Frailty Assessment
Authors: Kumar Danya Pradeep , Toosizadeh Nima , Mohler Jane , Ehsani Hossein , Mannier Cassidy , Laksari Kaveh 
Published: April 2020

Abstract
Background : Frailty is a highly recognized geriatric syndrome resulting in decline in reserve across multiple physiological systems. Impaired physical function is one of the major indicators of frailty. The goal of this study was to evaluate an algorithm that discriminates between frailty groups (non-frail and pre-frail/frail) based on gait performance parameters derived from unsupervised daily physical activity (DPA).
Methods : DPA was acquired for 48 hours from older adults (≥65 years) using a tri-axial accelerometer motion-sensor. Continuous bouts of walking for 20s, 30s, 40s, 50s and 60s without pauses were identified from acceleration data. These were then used to extract qualitative measures (gait variability, gait asymmetry, and gait irregularity) and quantitative measures (total continuous walking duration and maximum number of continuous steps) to characterize gait performance. Association between frailty and gait performance parameters was assessed using multinomial logistic models with frailty as the dependent variable, and gait performance parameters along with demographic parameters as independent variables.
Results : 126 older adults (44 non-frail, 60 pre-frail, and 22 frail, based on the Fried index) were recruited. Step- and stride-times, frequency domain gait variability, and continuous walking quantitative measures were significantly different between non-frail and pre-frail/frail groups ( p <0.05). Among the five different durations (20s, 30s, 40s, 50s and 60s), gait performance parameters extracted from 60s continuous walks provided the best frailty assessment results. Using the 60s gait performance parameters in the logistic model, pre-frail/frail group (vs. non-frail) was identified with 76.8% sensitivity and 80% specificity.
Discussion : Everyday walking characteristics were found to be associated with frailty. Along with quantitative measures of physical activity, qualitative measures are critical elements representing the early stages of frailty. In-home gait assessment offers an opportunity to screen for and monitor frailty.
Tongue fissure visualization by using deep learning – an example of the application of artificial intelligence in traditional medicine
Authors: Chang Wen-Hsien , Wu Han-Kuei , Lo Lun-chien , Hsiao William W. L. , Chu Hsueh-Ting , Chang Hen-Hong 
Published: April 2020

Abstract
Background
Traditional Chinese medicine (TCM) describes physiological and pathological changes inside and outside the human body by the application of four methods of diagnosis. One of the four methods, tongue diagnosis, is widely used by TCM physicians, since it allows direct observations that prevent discrepancies in the patient’s history and, as such, provides clinically important, objective evidence. The clinical significance of tongue features has been explored in both TCM and modern medicine. However, TCM physicians may have different interpretations of the features displayed by the same tongue, and therefore intra- and inter-observer agreements are relatively low. If an automated interpretation system could be developed, more consistent results could be obtained, and learning could also be more efficient. This study will apply a recently developed deep learning method to the classification of tongue features, and indicate the regions where the features are located.
Methods
A large number of tongue photographs with labeled fissures were used. Transfer learning was conducted using the ImageNet-pretrained ResNet50 model to determine whether tongue fissures were identified on a tongue photograph. Often, the neural network model lacks interpretability, and users cannot understand how the model determines the presence of tongue fissures. Therefore, Gradient-weighted Class Activation Mapping (Grad-CAM) was also applied to directly mark the tongue features on the tongue image.
Results
Only 6 epochs were trained in this study and no graphics processing units (GPUs) were used. It took less than 4 minutes for each epoch to be trained. The correct rate for the test set was approximately 70%. After the model training was completed, Grad-CAM was applied to localize tongue fissures in each image. The neural network model not only determined whether tongue fissures existed, but also allowed users to learn about the tongue fissure regions.
Conclusions
This study demonstrated how to apply transfer learning using the ImageNet-pretrained ResNet50 model for the identification and localization of tongue fissures and regions. The neural network model built in this study provided interpretability and intuitiveness, (often lacking in general neural network models), and improved the feasibility for clinical application.
Oil supplementation with a special combination of n-3 and n-6 long-chain polyunsaturated fatty acids does not protect for exercise induced asthma. A double-blind placebo-controlled trial.
Authors: Dreßler Melanie , Fussbroich Daniela , Böhler Lisa , Herrmann Eva , Benker Nicole , Tytyk Markus , Schulze Johannes , Schubert Ralf , Beermann Christopher , Zielen Stefan 
Published: April 2020

Abstract
Background Many patients suffering from exercise-induced asthma (EIA) have normal lung function at rest and show symptoms and a decline in FEV 1 when they do sports or during exercise-challenge. It has been described that long-chain polyunsaturated fatty acids (LCPUFA) could exert a protective effect on EIA. Methods In this study the protective effect of supplementation with a special combination of n-3 and n-6 LCPUFA (sc-LCPUFA) (total 1.19 g/ day) were investigated in an EIA cold air provocation model. Primary outcome measure: Decrease in FEV 1 after exercise challenge and secondary outcome measure: anti-inflammatory effects monitored by exhaled NO (eNO) before and after sc-LCPUFA supplementation versus placebo. Results 99 patients with exercise-induced symptoms aged 10 to 45 were screened by a standardized exercise challenge in a cold air chamber at 4 °C. 73 patients fulfilled the inclusion criteria of a FEV 1 decrease >15% and were treated double-blind placebo-controlled for four weeks either with sc-LCPUFA or placebo. 32 patients in each group completed the study. Mean FEV 1 decrease after cold air exercise challenge and eNO were unchanged after four weeks sc-LCPUFA supplementation. Conclusion Supplementation with sc-LCPUFA at a dose of 1.19 g/d did not have any broncho-protective ant anti-inflammatory effects on EIA.
Gut Microbial Dysbiosis in Individuals with Sjögren’s Syndrome
Authors: Mendez Roberto , Watane Arjun , Farhangi Monika , Cavuoto Kara M. , Leith Tom , Budree Shrish , Galor Anat , Banerjee Santanu 
Published: April 2020

Abstract
Background: Autoimmune diseases have been associated with changes in the gut microbiome. In this study, the gut microbiome was evaluated in individuals with dry eye and bacterial compositions were correlated to dry eye (DE) measures. We prospectively included 13 individuals with who met full criteria for Sjögren’s (SDE) and 8 individuals with features of Sjögren’s but who did not meet full criteria (NDE) for a total of 21 cases as compared to 21 healthy controls. Stool was analyzed by 16S pyrosequencing, and associations between bacterial classes and DE symptoms and signs were examined. Results: Results showed that Firmicutes was the dominant phylum in the gut, comprising 40-60% of all phyla. On a phyla level, subjects with DE (SDE and NDE) had depletion of Firmicutes (1.1-fold) and an expansion of Proteobacteria (3.0-fold), Actinobacteria (1.7-fold), and Bacteroidetes (1.3-fold) compared to controls. Shannon’s diversity index showed no differences between groups with respect to the numbers of different operational taxonomic units (OTUs) encountered (diversity) and the instances these unique OTUs were sampled (evenness). On the other hand, Faith’s phylogenetic diversity showed increased diversity in cases vs controls, which reached significance when comparing SDE and controls (13.57 ± 0.89 and 10.96 ± 0.76, p=0.02). Using Principle Co-ordinate Analysis, qualitative differences in microbial composition were noted with differential clustering of cases and controls. Dimensionality reduction and clustering of complex microbial data further showed differences between the three groups, with regard to microbial composition, association and clustering. Finally, differences in certain classes of bacteria were associated with DE symptoms and signs. Conclusions: In conclusion, individuals with DE had gut microbiome alterations as compared to healthy controls. Certain classes of bacteria were associated with DE measures.
First report of Lihan Tick virus (Phlebovirus, Phenuiviridae) in ticks, Colombia
Authors: Lopez Yesica , Miranda Jorge , Mattar Salim , Gonzalez Marco , Rovnak Joel 
Published: April 2020

Abstract
Background: Tick-borne phenuivirus (TBPVs) comprise human and animal viruses that can cause a variety of clinical syndromes ranging from self-limiting febrile illness to fatal haemorrhagic fevers. Objective: Detect Phlebovirus (Family Phenuiviridae) in ticks collected from domestic animals in Córdoba, Colombia. Methods: We collected 2,365 ticks from domestic animals in three municipalities of the Department of Cordoba, Colombia in 2016. Ticks were identified and pooled by species for RNA extraction. A nested real-time PCR with specific primers for Phlebovirus and a specific probe for Heartland virus (HRTV) formerly a Phlebovirus, now a Banyangvirus were performed. Also, a conventional nested PCR, with the same specific primers was used to detect other Phleboviruses , with positive reactions indicated by an amplified cDNA fragment of approximately 244 bp determined by gel electrophoresis. These bands were gel-purified and sequenced by the Sanger method. Results: Using real-time RT-PCR, no positive results for HRTV were found. However, using conventional nested PCR 2.2% (5/229 pools) yielded a product of 244 bp. One positive sample was detected in a pool of Dermacentor nitens ticks collected from a horse, and the four remaining positive pools were from Rhipicephalus microplus collected from cattle. The five positive nucleotide sequences had identities of 93 to 96% compared to a section of the L-segment of Lihan Tick virus , a Phlebovirus originally detected in R. microplus ticks in China. The strongest identity (96 -99%) was with Lihan Tick virus detected in R. microplus ticks from Brazil. Conclusions: This is the first report of viral detection in ticks in Colombia. We detected a Colombian strain of Lihan Tick virus . We recommend expanding the sampling area and carrying out more eco-epidemiological studies related to epidemiological surveillance of viruses on ticks in Colombia.
DL-3-n-butylphthalide improves cerebral hypoperfusion in patients with large cerebral atherosclerotic stenosis. A single-center, randomized, double-blind, placebo-controlled study.
Authors: Chen Dawei , Yin Yanwei , Shi Jin , Yang Fen , Wang Kehua , Zhao Faguo , Li Wenping , Li Bin 
Published: April 2020

Abstract
Background: DL-3-n-butylphthalide (NBP) was demonstrated to increase the cerebral blood flow (CBF) in the animal models, but there are no clinic studies to verify this. We aimed to explore the effect of NBP on improving cerebral hypoperfusion caused by cerebral large-vessel stenosis.
Methods: In this single-center, randomized, double-blind, placebo-controlled study, 120 patients with severe carotid atherosclerotic stenosis and cerebral hypoperfusion in the ipsilateral middle cerebral artery (MCA) were included and randomly assigned into NBP or placebo group as 1:1 radio. Patients in NBP or placebo group received 200mg or 20mg of NBP capsules three times daily for four weeks respectively. Single photon emission computed tomography (SPECT) was used to assess regional CBF (rCBF) in four regions of interest (ROIs) corresponding to MCA before and 12 weeks after the treatment. After therapy, the rCBF change for every ROI and the whole CBF change in MCA territory for every patient were classified into amelioration, stabilization and deterioration respectively.
Results: 48 NBP patients (6 with bilateral stenosis) and 46 placebo patients (8 with bilateral stenosis) completed the trial. Overall, both groups had 54 stenotic carotid arteries and 216 ROIs for rCBF change analysis. After therapy, the rCBF in ROIs increased in NBP group (83.5%±11.4% vs. 85.8%±12.5%, p=0.000), whereas no change was found in placebo group (86.9%±11.6% vs. 87.8%±11.7%, p=0.331). Besides, there was higher percentages of ROIs with rCBF amelioration and stabilization in NBP group than in placebo group (93.1% vs. 79.2%, p=0.000). Furthermore, ordinal regression analysis showed that compared with placebo, NBP independently made more patients to have whole CBF amelioration in ipsilateral MCA (Wald-χ2=5.247, OR=3.31, p=0.022).
Conclusions: NBP might improve the cerebral hypoperfusion in the patients with carotid artery atherosclerotic stenosis.
Trial registration: Chinese Clinical Trial Registry, ChiCTR1900028005, registered December 8th 2019- Retrospectively registered ( http://www.chictr.org.cn/index.aspx ).
Social, Economic, and Legislative Factors and Global Road Traffic Fatalities
Authors: Haghighi Mohammad Reza Rahmanian , Sayari Mohammad , Ghahramani Sulmaz , Lankarani Kamran Bagheri 
Published: April 2020

Abstract
Background: Road traffic fatalities (RTF) is the 8th cause of mortality around the world. At the end of the Decade of Action, it would be of utmost importance to revisit our knowledge on the determinants of RTF.Methods: We used Road Safety Development Index (RSDI) which accounts for the interactions between system, human and products to assess the RTF in 115 and 113 countries in 2013 and 2016, respectively. To analyze data, three statistical procedures (linear regression, Classification and Regression Trees (CART), and Multivariate Adaptive Regression Splines (MARS)) were employed. Results: CART has the best performance amongst all others followed by MARS for 2013 and 2016 data set with an R 2 around 0.83. Results show that any increase in human development index (HDI) was associated with RTF reduction. Comparing RTF data of 2013 and 2016, 8 countries experienced a change of more than 30%. RTF change in these countries showed significant relationship with GINI index. Considering the three components of HDI, it is revealed that education explained most of RTF variation in CART model followed by income and life expectancy. Conclusion: Policy makers can make provisions to reduce RTF in the long run by focusing on enhancing the three components of HDI, mainly education. However, there is a need to investigate the correlation among these three components with RTF with different time-trend procedures.
Prescription of antibiotics to children with acute otitis media in Danish general practice
Authors: Olsen Jonas , Lykkegaard Jesper , Hansen Malene Plejdrup , Waldorff Frans B , Lous Jørgen , Andersen Merethe K 
Published: April 2020

Abstract
Background: Acute otitis media (AOM) is a common and most often self-limiting infection in childhood, usually managed in general practice. Even though antibiotics are only recommended when certain diagnostic and clinical criteria are met a high antibiotic prescription rate is observed. The study’s objective was to analyse associations between patient- and general practitioner (GP) characteristics and antibiotic prescribing for children with AOM in an effort to explain the high antibiotic prescribing rates.Methods: All general practices in the Northern, Southern and Central regions of Denmark were invited to record symptoms, examinations, findings and antibiotic treatment for all children ≤ 7 years of age diagnosed with AOM during a four-week winter period in 2017/2018. Associations were analysed by means of multivariate logistic regressions. The study design was cross-sectional.Results: In total, 60 general practices diagnosed 278 children with AOM of whom 207 (74%) were prescribed antibiotics, most often penicillin V (60%). About half of the children had tympanometry performed. Antibiotic prescribing rates varied considerably between practices (0-100%). Antibiotic prescribing was associated with fever (odds ratio (OR) 3.69 95% confidence interval (CI) 1.93-7.05), purulent ear secretion (OR 2.35 95% CI 1.01-5.50) and poor general condition (OR 3.12 95% CI 1.31-7.46), and the practice’s antibiotic prescribing rate (highest vs lowest quarter) to patients with symptoms of an acute respiratory tract infection (OR 2.85 CI 95% 1.07-7.60) and specifically to children with AOM (OR 4.15 CI 95% 1.82-9.47).Conclusion: GPs' antibiotic prescribing rates for children with AOM vary considerably even considering the of signs, symptoms, request for antibiotics, and use of tympanometry. Interventions to reduce overprescribing should be targeted high-prescribing practices.