bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-Cell Transcriptomic Landscape Reveals the Differences in Cell Differentiation and Immune Microenvironment of Papillary Thyroid Carcinoma Between Genders
Authors: Peng Miaoguan , Wei Guohong , Zhang Yunjian , Li Hai , Lai Yingrong , Guo Yan , Chen Yuxin , Liu Liehua , Xiao Haipeng , Guan Hongyu , Li Yanbing 
Published: December 2020

Abstract
BackgroundPapillary thyroid carcinoma (PTC) is the main pathological type of thyroid carcinoma (TC). Gender is a prominent background parameter for patients with PTC. Here, we aimed to delineate the differences in cell clusters and immune microenvironment in relation to gender in PTC.ResultsMalignant epithelial cells were divided into two distinct subsets in male and female patients with PTC. Moreover, significant differences involving copy-number variations (CNVs), gene profiles, and cell differentiation were detected between male and female patients. Regarding the interactions of fibroblasts and endothelial cells with malignant epithelial cells, members of the human leukocyte antigen (HLA) family and their receptors were considered as typical in female patients with PTC, while transforming growth factor beta 1 (TGFB1) and its receptors were typical of male patients with PTC. The characteristics of B cells, including cell clusters, cell differentiation, and dominant gene sets, were significantly different between genders. ConclusionsOur data revealed the detailed differences in cell clusters and immune microenvironment in PTC according to gender at the single-cell level, which provided new insights into the understanding of the impact of gender on PTC.
Trained Immunity Induced by in Vivo Peptide-Based STAT6&nbsp;Inhibition Prevents Ragweed Allergy in Mice
Authors: Michael Husheem , Li Yuanyi , Wang Yufa , McCusker Christine T. 
Published: December 2020

Abstract
BackgroundAllergic airways disease (AAD) is initiated, maintained by the type 2 (T2) inflammatory pathway, and is partially regulated by cytokines IL-4 and IL-13 following activation of the STAT6 transcription factor. ObjectiveTo investigate mucosal immune responses, using neonatal vaccination with the STAT6 inhibitory peptide (STAT6-IP), to prevent the development of ragweed-induced AAD. MethodsWe demonstrate that transfer of CD4+ T cells or dendritic cells (DC) from STAT6-IP vaccinated wild-type BALB/c mice to naïve mice, that were subsequently chronically exposed to sensitizing doses of ragweed allergen, is sufficient to prevent development of T2 responses in recipients. ResultsOur results demonstrate significant reductions in; airways hyperresponsiveness (AHR); ragweed-specific IgE; pulmonary inflammation; T2 cytokines; and inflammatory gene expressions in recipient mice. Expression of IDO, TGFβ and T regulatory cells were all significantly increased. Anti-TGFβ treatment during the ragweed sensitization phase re-constituted the pro-inflammatory T2 immune response. We show that tolerance can be attained via DC or T cells trained in the STAT6-IP-mediated tolerant milieu. This effect is not restricted to a particular allergen and does not require antigen-mediated T cell activation prior to transfer. ConclusionThese data indicate that early transient STAT6-inhibition constitutes an effective immunomodulatory airways allergy preventative strategy.
Elevated Exosome-derived Mirnas Predict Osimertinib Resistance in Non-small Cell Lung Cancer
Authors: Li Xinying , Chen Cen , Wang Zimu , Liu Jiaxin , Sun Wei , Shen Kaikai , Lv Yanling , Zhu Suhua , Zhan Ping , Lv Tangfeng , Song Yong 
Published: December 2020

Abstract
Background: Non-small cell lung cancer (NSCLC) patients with epidermal growth factor receptor (EGFR) mutations will inevitably develop drug resistance after being treated with the third-generation EGFR-tyrosine kinase inhibitor (TKI), osimertinib. In recent years, many studies have been focusing on the ability of exosomal miRNAs secreted by tumor cells to transmit resistance information. However, the mechanism of exosome-derived miRNAs in osimertinib resistance remains unexplored.Methods: We extracted and sequenced exosomes from the supernatant of the osimertinib-resistant cell line, H1975-OR, and the sensitive cell line, H1975. The results were compared with plasma exosome sequencing before and after the appearance of drug resistance in three NSCLC clinical patients treated with oral osimertinib. Exosome-derived miRNAs that had significantly increased expression levels after osimertinib resistance were screened for expanded validation in other 64 NSCLC patients.Results: Cluster analysis of the target genes revealed that exosomal miRNAs participate in osimertinib resistance mechanisms through the activation of bypass pathways (RAS-MAPK pathway abnormality and PI3K pathway activation). Exosome-derived miR-184 (p = 0.0325) and miR-3913-5p (p = 0.0169) expression levels increased significantly after the onset of osimertinib resistance. Exosomal miR-184 was correlated with lactate dehydrogenase levels (p = 0.018). Exosomal miR-3913-5p was associated with TNM stage (p = 0.045), platelet count (p = 0.024), tumor marker carcinoembryonic antigen (p = 0.045), and distant metastases (p = 0.049), especially bone metastasis (p = 0.03). In the subgroup of patients with EGFR exon 21 L858R point mutation, miR-184 (p = 0.0104) and miR-3913-5p (p = 0.0085) derived from serum exosomes had both significantly increased expression levels. In the subgroup of T790M-positive patients, miR-3913-5p derived from serum exosomes may also be a good indicator of osimertinib resistance (p = 0.013).Conclusions: The expression levels of miR-184 and miR-3913-5p derived from exosomes in the peripheral blood of NSCLC patients could be used as biomarkers to indicate osimertinib resistance.
Effects of Metformin on Reproductive, Endocrine, and Metabolic Characteristics of Female Offspring in a Rat Model of Letrozole-induced Polycystic Ovarian Syndrome With Insulin Resistant
Authors: Xie Yidong , Xiao Li , Shi Xiaohan , Zhao Yifan , Li Xiaohong , Wang Yan , Qin Lang , Li Shangwei 
Published: December 2020

Abstract
Background: Polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine disorder that has many characteristic features including hyperandrogenemia, insulin resistance and obesity. The beneficial effects of metformin on reproduction, metabolism and endocrine, especially with the capacity to ameliorate insulin resistance (IR) in polycystic ovary syndrome (PCOS), place it as a good alternative for its widely prescribed, but its association with PCOS offspring remains uncertain. We aim to investigate the impact of metformin on reproductive, endocrine and metabolic characteristics in female offspring born to letrozole-induced PCOS-IR rats.Methods: 45 female wistar rats were implanted with letrozole-continuous-release pellets or placebo, subsequently treated with metformin or vehicle control, then mated with healthy male wistar rats. Estrous cycle, endocrine hormone profile, fasting insulin measurements and glucose tolerance test have been investigated and the expression of INSR in pancreas, FSHR and LHCGR in ovaries have been analyzed with Quantitative Real-time Polymerase Chain Reaction and Western Blotting assay.Results: Decreased conception rate and increased multiple pregnancy rates were found in PCOS-IR rats, which significantly improved after metformin treatment. Metformin significantly decreased the risk of body weight gain and increased INSR expression of pancreas in female F1 offspring in PCOS-IR rats. Decreased FSHR and increased LHCGR expressions in ovary were observed in female F1 rats of PCOS-IR and PCOS-IR+Met group. Nevertheless, there were no significant differences of INSR, FSHR and LHCGR expressions in female F2 offspring of PCOS-IR rats, as well as other PCOS phenotypes.Conclusions: The current study indicates that widespread reproductive, endocrine and metabolic changes in letrozole induced PCOS-IR rat model, but those PCOS phenotypes could not be inherit to offspring generations stably. Metformin may contribute to improve obesity, hyperinsulinemia and insulin resistance of female F1 offspring in PCOS-IR. The results of this study can be used as a theoretical basis for supporting metformin-using in the treatment of PCOS-IR patients.
Towards A Comprehensive Breastfeeding-Friendly Workplace Environment: Insight From Selected Health Facilities In The Central Region of Ghana
Authors: Nkrumah Jacqueline N/A , Abuosi Aaron Asibi , Nkrumah Rodney Buadi 
Published: December 2020

Abstract
Background In the last three decades, Ghana has championed the objectives of the Baby Friendly Hospital Initiative to provide pregnant women and nursing mothers with skills and support necessary for optimal breastfeeding. Yet, little is known about practical interventions to promote breastfeeding friendly work environment in healthcare facilities. This study explores the extent to which healthcare facilities in Effutu Municipality provide breastfeeding friendly workplace environment to breastfeeding frontline health workers. Methods A descriptive mixed-method approach was employed to collect data from healthcare facility representative and breastfeeding frontline health workers. Self-administered questionnaire with structured responses and interview guide were used to collect data. Thematic analysis was used to analyze interview responses. Survey responses were processed with SPSS version 23.0 and presented using frequencies and percentages.Results Three main themes, namely, Standpoints on workplace breastfeeding support; Breastfeeding support, suggested future directions and six sub-themes, including backings for workplace breastfeeding support; perceived benefits of breastfeeding support; factors of poor breastfeeding workplace support; maternity protection benefits; workplace support gaps, and awareness creation on benefits were identified to explain the extent to which health facilities provide breastfeeding friendly workplace environment. Breastfeeding frontline health workers said their hospitals have no breastfeeding policy (96%), no breastfeeding facility (96%), do not go to work with baby (96%), but went on 12 weeks maternity leave (96%) and work half-day (70%) on return to work.ConclusionHealth facilities in the study do not provide breastfeeding friendly work environment except the privileges provided by the Labor Act. Continuous advocacy on breastfeeding workplace support and stakeholder engagement to build consensus on the mix of strategies suitable to cushion breastfeeding frontline health workers against work-breastfeeding stress and minimize its negative impact on optimal breastfeeding among frontline health workers is recommended.
Investigating Health Status Among Women Aged 50-70 years old in Hunan Province, China: A Cross-Sectional study
Authors: Luo Yang , Min NIE , Yan-ting MENG , Chen XU , Si QIN , Ling FAN , Jing YUE 
Published: December 2020

Abstract
Background: As promotion of the Global strategy and action plan on ageing and health 2016-2020 and the Outline of the "Healthy China 2030" Plan, healthy ageing becomes a hot pot. It is crucial to identify health status of senior women and acquire characteristics of them. The purpose of this paper was to investigate the health of women aged 50-70 in Hunan Province of China, come out coping strategy and prompt an active discussion for healthy ageing.Methods: A cross-sectional study was conducted in 20 communities randomly selected from 5 sampled districts and 5counties in Hunan Province. A self-designed and structured questionnaire was used, included demographic data, diseases-related information, self-reported health, health behaviors, and psychological health. The data was collected by well-trained workers, double-entered into EpiData 3.0 and analyzed by SPSS 19.0.Results: Among the 2585 senior women, 51.0% was chronic diseases, 49.6% was gynecopathy and 23.6% was mastopathy. Age [OR=1.394, 95% CI=(1.63-1.670)], household type [OR=1.700, 95% CI=(1.416-2.042)], BMI [OR=1.194, 95% CI=(1.043-1.367)], average monthly household income [OR=0.884, 95% CI=(0.809-0.965)] and self-reported physical health [OR=4.659, 95% CI=(3.913-5.546)] were significantly associated with chronic diseases. Age [OR=0.668, 95% CI=(0.564-0.792)], household type [OR=1.335, 95% CI=(1.094-1.629)], occupation [OR=1.045, 95% CI=(1.002-1.090)], average monthly household income [OR=1.243, 95% CI=(1.143-1.352)] and self-reported physical health [OR=1.748, 95% CI=(1.510-2.024)] were significantly associated with gynecopathy. Age [OR=0.749, 95% CI=(0.616-0.910)], household type [OR=1.603, 95% CI=(1.269-2.026)], occupation [OR=1.057, 95% CI=(1.009-1.108)], average monthly household income [OR=1.233, 95% CI=(1.127-1.350)] and self-reported physical health [OR=1.272, 95% CI=(1.077-1.502)] were significantly associated with mastopathy. Age [OR=0.778, 95% CI=(0.645-0.939)], household type [OR=2.090, 95% CI=(1.733-2.520)], and BMI [OR=1.218, 95% CI=(1.061-1.399)] were significantly associated with exposure to secondhand smoke. Age [OR=0.829, 95% CI=(0.701-0.980)] and occupation [OR=0.956, 95% CI=(0.923-0.990)] were significantly associated with regular physical examinations. Household type [OR=0.642, 95% CI=(0.398-1.035)] and self-reported physical health [OR=1.983, 95% CI=(1.340-2.935)] were significantly associated with anxiety.Conclusion: This cross-sectional study shows good description as an appropriate investigation for the identification of women aged 50-70 with certain diseases, health behaviors and psychological health problems in a representative and community-conducted sample of women in Hunan Province of China. It discloses their poor health status.
Economic Conditions and Mental Health in Thailand
Authors: Lerskullawat Attasuda , Puttitanun Thitima 
Published: December 2020

Abstract
BackgroundThere is a continual rise in the number of people suffered from mental disorder around the world. Several factors are believed to be connected to the development of mental disorders. Apart from health factors, mental disorders could also be caused by the economic and social factors associated with the patients. However, none of the existing literature has provided the analysis by taking into account all the possible causes together. This paper attempts to fill this gap in the literature by ascertaining the relationship between economic and social factors in relation to mental disorders.MethodsUsing the number of mental disorder cases in all 77 provinces in Thailand during the period 2015 to 2017, this paper uses either a fixed effects or a random effects model, depending on the results of the Hausman test, to analyse the effect of economic and social factors on the number of mental disorder cases in Thailand.ResultsGenerally, we found that better economic situations, as measured by higher GPP per capita, higher employment rates and lower household debt, help reduce mental disorder rates, while social factors such as greater health service accessibility and lower technology accessibility also reduce the number of mental disorder cases. However, death and divorce rates are not consistent in their effect on mental disorder rates, their impact seeming to depend on what type of mental disorder is being considered. ConclusionsWhen considering different types of disorders, we found that they could be affected by each of these factors in different ways.
Socio-Economic Consequences of Mental Distress: Quantifying the Impact of Self-Reported Mental Distress on the Days of Incapacity to Work and Medical Costs in the Following 2 years: A Longitudinal Study in Germany
Authors: Müller Gerhard , Bombana Manuela , Heinzel-Gutenbrenner Monika , Kleindienst Nikolaus , Bohus Martin , Lyssenko Lisa , Vonderlin Ruben 
Published: December 2020

Abstract
Background: Mental disorders are related to high individual suffering and significant socio-economic burdens. However, it remains unclear to what extent self-reported mental distress is related to individuals’ days of incapacity to work and their medical costs. This study aims to investigate the impact of self-reported mental distress for specific and non-specific days of incapacity to work and specific and non-specific medical costs over a two-year span.Method: Within a longitudinal research design, 2,287 study participants' mental distress was assessed using the Hospital Anxiety and Depression Scale (HADS). Days of incapacity to work and medical costs were retrieved from a German health insurance’s routine data during the following two-year period.Results: Current mental distress was found to be significantly related to the number of specific days absent from work and medical costs. Compared to participants classified as no cases by the HADS (2.6 days), severe case participants showed 27.3-times as many specific days of incapacity to work in the first year (72 days) and 10.3-times as many days in the second year (44 days), and resulted in 11.4-times more medical costs in the first year (2272 EUR) and 6.2-times more in the second year (1319 EUR). The relationship of mental distress to non-specific days of incapacity to work and non-specific medical costs is also significant, but mainly driven from specific absent days and specific medical costs. Our results also indicate that the prevalence of presenteeism is considerably high: 42% of individuals continued to go to work despite severe mental distress. Conclusions: Our results show that self-reported mental distress, assessed by the HADS, is highly related to the days of incapacity to work and medical costs in the two-year period. Reducing mental distress by improving preventive structures for at-risk populations and increasing access to evidence-based treatments for individuals with mental disorders might, therefore, pay for itself and could help to reduce public costs.
Midday Meals as an Early Childhood Nutrition Intervention: Evidence from Plantation Communities in Sri Lanka
Authors: Perera Udeni De Silva , Inder Brett A. 
Published: December 2020

Abstract
Background: High rates of child malnutrition are a major public health concern in developing countries, particularly among vulnerable communities. Midday meals programs can be effective for combatting childhood malnutrition among older children. However, their use in early childhood is not well documented, particularly within South Asia. Anthropometric measures and other socioeconomic data were collected for children below the age of five years living in selected Sri Lankan tea plantations, to assess the effectiveness of midday meals as a nutrition intervention for improving growth among young children.Methods: The study exploits a natural experiment whereby the provision of the midday meals program is exogenously determined at the plantation level, resulting in comparable treatment and control groups. Longitudinal data is regularly collected on heights and weights of children, between 2013-2015. Standardized weight-for-age, height-for-age, BMI-for-age and weight-for-height are calculated following WHO guidelines, and binary variables for stunting, wasting and underweight are constructed. All modelling uses STATA SE 15. Random-effects regression with instrumental variables is used for modelling standardized growth variables whilst random-effects logistic regression is used for binary outcome variables. Robustness analysis involves different estimation methods and subsamples. Results: The dataset consists of longitudinal data from a total of 1279 children across three tea plantations in Sri Lanka, with 799 children in the treatment group and 480 in the control group. Results show significant positive effects of access to the midday meals program, on the growth of children. A child with access to the midday meals intervention reports an average standardized weight-for-age 0.03 (±0.01) and height-for-age 0.05 (±0.01) units higher than a similar child without access to the intervention. Importantly, access to the intervention reduces the likelihood of being underweight by 0.45 and the likelihood of wasting by 0.47. The results are robust to different model estimations and across different subsamples by gender, birthweight and birth-year cohort. Qualitative data analysis suggests a high viability of implementing similar programs within tea plantations in Sri Lanka.Conclusions: Midday meals programs targeting early childhood can be an effective intervention to address high rates of child malnutrition, particularly among vulnerable communities in developing countries like Sri Lanka.
Change in Work-Related Income Following the Uptake of Treatment for Mental Disorders Among Young Migrant Women in Norway: A National Register Study
Authors: Hynek Kamila Angelika , Straiton Melanie Lindsay , Hollander Anna-Clara , Liefbroer Aart , Hauge Lars Johan 
Published: December 2020

Abstract
Background: Mental disorders are associated with several work-related outcomes such as loss of income. However, studies investigating the effect of mental disorders on work-related income by migrant background are lacking. This study aimed to assess the change in work-related income following the uptake of outpatient mental healthcare (OPMH) treatment, a proxy for mental disorder, in young women with migrant background. Additionally, we looked at how this varied by income level.Methods: This study makes use of data from several national registries. The study population consisted of women aged 23-40 years residing in Norway for at least three consecutive years between 2006 and 2013 (N=640,527). By using a stratified linear regression with individual fixed effects, we investigated differences between the majority women, descendants and eight migrant groups. Interaction analysis was conducted in order to examine the differences in income loss following the uptake of OPMH treatment among women with and without migrant background. Results: Results show that OPMH treatment was associated with a decrease in income for all groups. However, the negative effect was stronger among those with low income. Only migrant women from EU Eastern Europe with a high income were not significantly affected following OPMH treatment. Conclusions: Experiencing a mental disorder during a critical age for establishment in the labour market may not only affect income but also subsequent workforce participation and dependency on social welfare services, regardless of migrant background, resulting in large economic costs for both the individual and for the society as a whole.
New neural network classification method for individuals ancestry prediction from SNPs data
Authors: Soumare Harouna , Rezgui Sadok , Benkahla Alia , Gmati Nabil 
Published: December 2020

Abstract
Artificial Neural Network (ANN) algorithms have been widely applied to genomic prediction data. Single Nucleotide Polymorphisms(SNPs) represent the most common genetic variations of human genome, it has been shown that they are implicated in many human diseases and they can be used to predict genetic diseases such as diabetes. Developing ANN techniques to handle such kind of data can be considered as a great success in medical field. However, the high dimensionality of genomic data and availability of only a few number of samples can make learning task very complicated. In this work, we propose a New Neural Network classification method based on input perturbation. The idea is first to use SVD to reduce the dimensionality of input data and train a classification network which prediction errors are then reduced by perturbing the SVD projection matrix. The proposed method has been evaluated on individuals genetic ancestry prediction dataset, the experimental results show the efficiency of proposed method. Reaching up to 96.23% of classification accuracy, this approach exceeds the previous deep learning approach evaluated on the same dataset.