bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hepatopancreas immune response during molt cycle in the mud crab, Scylla paramamosain
Authors: Xu Zhanning , Liu An , Li Shengkang , Wang Guizhong , Ye Haihui 
Published: August 2020

AbstractMolt is a critical developmental process in crustaceans. Recent studies have shown that the hepatopancreas is an important source of innate immune molecules, yet hepatopancreatic patterns of gene expression during the molt cycle which may underlie changes in immune mechanism are unknown. In this study, we performed Illumina sequencing for the hepatopancreas of the mud crab,Scylla paramamosainduring molt cycle (pre-molt stage, post-molt stage, and inter-molt stage). A total of 44.55 Gb high-quality reads were obtained from the normalized cDNA of hepatopancreas. A total of 70,591 transcripts were assembled; 55,167 unigenes were identified. Transcriptomic comparison revealed 948 differentially expressed genes (DEGs) in the hepatopancreas from the three molt stages. We found that genes associated with immune response patterns changed in expression during the molt cycle. Antimicrobial peptide genes, inflammatory response genes, Toll signaling pathway factors, the phenoloxidase system, antioxidant enzymes, metal-binding proteins and other immune related genes are significantly up-regulated at the post-molt stage and inter-molt stage compared with the pre-molt stage, respectively. These genes are either not expressed or are expressed at low levels at the pre-molt stage. To our knowledge, this is the first systematic transcriptome analysis of genes capable of mobilizing a hepatopancreas immune response during the molt cycle in crustaceans, and this study will contribute to a better understanding of the hepatopancreas immune system and mud crab prophylactic immune mechanisms at the post-molt stage.
In vitro screening of a FDA approved chemical library reveals potential inhibitors of SARS-CoV-2 replication
Authors: Touret Franck , Gilles Magali , Barral Karine , Nougairède Antoine , van Helden Jacques , Decroly Etienne , de Lamballerie Xavier , Coutard Bruno 
Published: August 2020

AbstractA novel coronavirus, named SARS-CoV-2, emerged in 2019 in China and rapidly spread worldwide. As no approved therapeutics exists to treat COVID-19, the disease associated to SARS-Cov-2, there is an urgent need to propose molecules that could quickly enter into clinics. Repurposing of approved drugs is a strategy that can bypass the time-consuming stages of drug development. In this study, we screened the PRESTWICK CHEMICAL LIBRARY composed of 1,520 approved drugs in an infected cell-based assay. The robustness of the screen was assessed by the identification of drugs that already demonstrated in vitro antiviral effect against SARS-CoV-2. Thereby, 90 compounds were identified as positive hits from the screen and were grouped according to their chemical composition and their known therapeutic effect. Then EC50 and CC50 were determined for a subset of 15 compounds from a panel of 23 selected drugs covering the different groups. Eleven compounds such as macrolides antibiotics, proton pump inhibitors, antiarrhythmic agents or CNS drugs emerged showing antiviral potency with 2 < EC50 ≤ 20 µM. By providing new information on molecules inhibiting SARS-CoV-2 replication in vitro, this study provides information for the selection of drugs to be further validated in vivo. Disclaimer: This study corresponds to the early stages of antiviral development and the results do not support by themselves the use of the selected drugs to treat SARS-CoV-2 infection.
Photocatalytic radical defluoroalkylation of unactivated alkenes via distal heteroaryl ipso-migration
Authors: Yuan Xin , Zhuang Kai-Qiang , Cui Yu-Sheng , Qin Long-Zhou , Sun Qi , Duan Xiu , Chen Lin , Zhu Ning , Li Guigen , Qiu Jiang-Kai , Guo Kai 
Published: August 2020

AbstractCurrently, the selective activation of C(sp3)–F bonds and C–C bonds constitute one of the most widely used procedures for the synthesis of high-value products that range from pharmaceuticals to agrochemical applications. While numerous examples of these two methods have been reported in their respective fields, the processes which merge the activation of both single C(sp3)-F bonds and C–C bonds in one step still remain elusive. Here, we demonstrate the controllable defluoroalkylation–distal functionalization of trifluoromethylarenes with unactivated alkenes via distal heteroaryl migration. This is proposed to proceed via tandem C(sp3)–F and C–C bond cleavage using visible-light photoredox catalysis combined with Lewis acid activation. This strategy provides facile and flexible access to multiply functionalizedα,α-difluorobenzylic ketones in useful yields (up to 88%) under mild conditions. The products can be further transformed into other valuable compounds, demonstrating the method’s utility.
A theoretical model of laser-driven ion acceleration from near-critical double-layer targets
Authors: Pazzaglia Andrea , Fedeli Luca , Formenti Arianna , Maffini Alessandro , Passoni Matteo 
Published: August 2020

AbstractLaser-driven ion sources are interesting for many potential applications, from nuclear medicine to material science. A promising strategy to enhance both ion energy and number is given by Double-Layer Targets (DLTs), i.e. micrometric foils coated by a near-critical density layer. Optimization of DLT parameters for a given laser setup requires a deep and thorough understanding of the physics at play. In this work, we investigate the acceleration process with DLTs by combining analytical modeling of pulse propagation and hot electron generation together with Particle-In-Cell (PIC) simulations in two and three dimensions. Model results and predictions are confirmed by PIC simulations—which also provide numerical values to the free model parameters—and compared to experimental findings from the literature. Finally, we analytically find the optimal values for near-critical layer thickness and density as a function of laser parameters; this result should provide useful insights for the design of experiments involving DLTs.
Dynamic Assessment Language Tasks and the Prediction of Performance on Year-End Language Skills in Preschool Dual Language Learners
Authors: Patterson Janet L. , Rodríguez Barbara L. , Dale Philip S. 
Published: August 2020

PurposeEarly identification is a key element for accessing appropriate services for preschool children with language impairment. However, there is a high risk of misidentifying typically developing dual language learners as having language impairment if inappropriate tools designed for monolingual children are used. In this study of children with bilingual exposure, we explored performance on brief dynamic assessment (DA) language tasks using graduated prompting because this approach has potential applications for screening. We asked if children's performance on DA language tasks earlier in the year was related to their performance on a year-end language achievement measure.MethodTwenty 4-year-old children from Spanish-speaking homes attending Head Start preschools in the southwestern United States completed three DA graduated prompting language tasks 3–6 months prior to the Head Start preschools' year-end achievement testing. The DA tasks, Novel Adjective Learning, Similarities in Function, and Prediction, were administered in Spanish, but correct responses in English or Spanish were accepted. The year-end achievement measure, the Learning Accomplishment Profile–Third Edition (LAP3), was administered by the children's Head Start teachers, who also credited correct responses in either language.ResultsChildren's performance on two of the three DA language tasks was significantly and positively related to year-end LAP3 language scores, and there was a moderate and significant relationship for one of the DA tasks, even when controlling for age and initial LAP3 scores.ConclusionsAlthough the relationship of performance on DA with year-end performance varies across tasks, the findings indicate potential for using a graduated prompting approach to language screening with young dual language learners. Further research is needed to select the best tasks for administration in a graduated prompting framework and determine accuracy of identification of language impairment.
Taxonomic Development in Young Bilingual Children: Task Matters, and So Does Scoring Method
Authors: Lam Boji P. W. , Sheng Li 
Published: August 2020

PurposeTaxonomic awareness is central to vocabulary development and assessment. While taxonomic development appears largely unaffected by environmental factors, the impact of divided language input on distinct levels of the taxonomic hierarchy is unclear. The influence of scoring method on tasks that target distinct levels of the taxonomic hierarchy is unexamined.MethodTwenty-seven English-speaking monolingual children, 46 Mandarin–English bilingual children, and 33 Spanish–English bilingual children, ages 4–7 years, participated. We measured superordinate awareness with a category association task, coordinate awareness with a contrast association task, and vocabulary size with a picture-naming task. All bilinguals completed the tasks in both languages to generate single-language (English) scores and conceptual scores.ResultsSingle-language scoring indicated that bilingual children named fewer pictures and produced fewer superordinate-level responses in English than monolinguals. All language groups demonstrated comparable coordinate awareness. Importantly, conceptual scoring removed the bilingual disadvantage in both naming and category association tasks and revealed a bilingual advantage in coordinate awareness. Finally, the Mandarin–English and Spanish–English bilingual children performed comparably in all analyses despite differences in heritage language features and sociocultural support for bilingual development.ConclusionDepending on task demand and scoring method, bilingual children exhibited slower, comparable, and faster development in taxonomic knowledge in comparison to monolingual controls. This study highlights the nuanced effect of bilingualism on different levels of the taxonomic hierarchy and the impact of scoring methods on measuring vocabulary depth.Supplemental Materialhttps://doi.org/10.23641/asha.12315683
Effects of a Supplemental Spanish Phonological Awareness Intervention on Latinx Preschoolers' Dual Language Emergent Literacy Skills
Authors: Soto Xigrid T. , Crucet-Choi Andres , Goldstein Howard 
Published: August 2020

Purpose
Preschoolers' phonological awareness (PA) and alphabet knowledge (AK) skills are two of the strongest predictors of future reading. Despite evidence that providing at-risk preschoolers with timely emergent literacy interventions can prevent academic difficulties, there is a scarcity of research focusing on Latinx preschoolers who are dual language learners. Despite evidence of benefits of providing Latinxs with Spanish emergent literacy instruction, few studies include preschoolers. This study examined the effects of a supplemental Spanish PA and AK intervention on the dual emergent literacy skills of at-risk Latinx preschoolers.

Method
A multiple probe design across four units of instruction evaluated the effects of a Spanish supplemental emergent literacy intervention that explicitly facilitated generalizations to English. Four Latinx preschoolers with limited emergent literacy skills in Spanish and English participated in this study. Bilingual researchers delivered scripted lessons targeting PA and AK skills in individual or small groups for 12–17 weeks.

Results
Children made large gains as each PA skill was introduced into intervention and generalized the PA skills they learned from Spanish to English. They also improved their English initial sound identification skills, a phonemic awareness task, when instruction was delivered in Spanish but with English words. Children made small to moderate gains in their Spanish letter naming and letter–sound correspondence skills and in generalizing this knowledge to English.

Conclusion
These findings provide preliminary evidence Latinx preschoolers who are dual language learners benefit from emergent literacy instruction that promotes their bilingual and biliterate development.
Orbital evolution of Saturn’s satellites due to the interaction between the moons and the massive rings
Authors: Nakajima Ayano , Ida Shigeru , Ishigaki Yota 
Published: August 2020

Context. Saturn’s mid-sized moons (satellites) have a puzzling orbital configuration with trapping in mean-motion resonances with every-other pairs (Mimas-Tethys 4:2 and Enceladus-Dione 2:1). To reproduce their current orbital configuration on the basis of a recent model of satellite formation from a hypothetical ancient massive ring, adjacent pairs must pass first-order mean-motion resonances without being trapped.
Aims. The trapping could be avoided by fast orbital migration and/or excitation of the satellite’s eccentricity caused by gravitational interactions between the satellites and the rings (the disk), which are still unknown. In our research we investigate the satellite orbital evolution due to interactions with the disk through full N-body simulations.
Methods. We performed global high-resolution N-body simulations of a self-gravitating particle disk interacting with a single satellite. We used N ∼ 105 particles for the disk. Gravitational forces of all the particles and their inelastic collisions are taken into account.
Results. Dense short-wavelength wake structure is created by the disk self-gravity and a few global spiral arms are induced by the satellite. The self-gravity wakes regulate the orbital evolution of the satellite, which has been considered as a disk spreading mechanism, but not as a driver for the orbital evolution.
Conclusions. The self-gravity wake torque to the satellite is so effective that the satellite migration is much faster than was predicted with the spiral arm torque. It provides a possible model to avoid the resonance capture of adjacent satellite pairs and establish the current orbital configuration of Saturn’s mid-sized satellites.
Frauen und Medizin im Laufe der Zeit
Authors: Flüchter Antje 
Published: August 2020

ZUSAMMENFASSUNGDer Aufsatz untersucht die Position von Frauen auf dem Feld der Medizin im Wandel der Zeit. Dies ist keine schlichte Fortschrittsgeschichte, vielmehr strukturieren die jeweiligen Geschlechterrollen aber auch die Ausgestaltung des medizinischen Felds weibliche Handlungsmöglichkeiten und ihre Grenzen. Die Darstellung beginnt im Mittelalter und verfolgt die Entwicklungen über die 2 zentralen Professionalisierungswellen, d. h. die Gründung der europäischen Universitäten seit dem 12. Jahrhundert und die Professionalisierung der Medizin im 19. Jahrhundert, und endet mit der schwierigen Etablierung von Ärztinnen in der ersten Hälfte des 20. Jahrhunderts.
I Will Be at Your (Bed)Side – The Role of Bedside Echocardiography for Non-Cardiologists
Authors: Casella Francesco , Schiavon Riccardo , Ceriani Elisa , Cogliati Chiara 
Published: August 2020

AbstractAs the availability of portable echocardiographic equipment is becoming more and more widespread, physicians can add a powerful tool to their bedside examination skills, thus helping them to more effectively face the growing complexity of patients admitted to internal medicine departments or the emergency room. Focused cardiac ultrasound (FoCUS) can be defined as a goal-directed, simplified, qualitative examination performed at the bedside using portable echocardiographic devices. FoCUS is not meant to be a substitute for a standard 2D color Doppler echocardiographic examination. Nevertheless, it can provide rapid and reliable information when limited to basic questions, even when performed by non-cardiologists with brief training. Furthermore, a focused cardiac ultrasound examination maximizes its diagnostic role when integrated with an ultrasonographic assessment of the lung, abdomen and deep veins, in a multisystem approach that is particularly dear to internists. In this article, we will focus on the specific targets of a focused cardiac ultrasound examination, as well as the most common pitfalls that can be encountered in ultrasonographic practice. We will also address the application of FoCUS in the management of two typical scenarios in clinical practice, such as dyspnea and non-post-traumatic hypotension.
Assessment of Aortic Elasticity and the Doppler Tissue Echocardiography in Thalassemia Major Children
Authors: Noori Noor Mohammad , Teimouri Alireza , Keshavarz Kambiz , Moradi Malihe 
Published: August 2020

AbstractCardiovascular diseases are the most common illness that needs special medical care in thalassemia particularly in children and adolescents. We aimed to compare aortic stiffness in thalassemia major and healthy children. The study included 65 asymptomatic thalassemia major 6 to 19-year–old children without known history of heart disease and 65 age- and sex-matched healthy controls. Arterial stiffness indices determined using two-dimensional (2D) and Doppler echocardiography. Data were analyzed by SPSS 20.0 with 0.05 as significant error. From the study, results showed that systolic (p = 0.009) and diastolic (p < 0.001) blood pressures were higher in controls. Left ventricular mass index (LVMI) was higher in patients (p < 0.001). Aortic stiffness index (p < 0.001), pulse pressure (p < 0.001), and pressure strain elastic modulus (p < 0.001) were higher in patients, while aortic strain (p < 0.002) and aortic distensibility (p < 0.001) were lower significantly. Aortic stiffness index was correlated with diastole aorta (p = 0.005), systole aorta (p < 0.001), and LVMI (p < 0.001). Strain was correlated with diastole aorta (p < 0.001). Pulse pressure was correlated with systolic blood pressure (p < 0.001), diastolic blood pressure (p = 0.002) significantly. Aortic distensibility was correlated with systolic blood pressure (p = 0.039) and diastole aorta (p < 0.001) significantly. The pressure strain elastic modulus was correlated only with diastole aorta (p = 0.029). Concluded, aortic stiffness index, pulse pressure, and pressure strain elastic modulus were higher in thalassemia children, while aortic strain and aortic distensibility were lower. This increase may result in reduction of mechanical efficiency of the heart. Therefore, assessment of aortic elastic properties as nontraditional cardiovascular risk factors may contribute to the identification of cardiovascular risks in children with thalassemia
Prevalence and Associated Factors with Mixed Coinfections among under 5-Year-Old Children with Severe Viral Pneumonia in Vietnam
Authors: Pham Hien T. , Nguyen Tran N. T. , Tran Quynh A. , Ngo Tam T. 
Published: August 2020

AbstractCommunity-acquired pneumonia (CAP) is well-recognized as a leading cause of disease burden in children. This study aimed to identify the prevalence of coinfection and associated factors in Vietnamese children ages 1 month to 5 years with viral pneumonia. We performed a cross-sectional study of children who were diagnosed with severe viral pneumonia. Demographic, clinical, and subclinical characteristics were compared between children with viral alone and bacterial coinfection. Multivariate logistic regression was used to determine which factors were associated with risk of coinfection. Of 202 children with severe viral pneumonia, the most common causative agent was respiratory syncytial virus (respiratory syncytial virus [RSV]: 36.1%), followed by influenza virus A (24.3%) and adenovirus (19.8%). Fifty-three children (26.2%) had bacterial superinfection and/or coinfection with other viruses. Haemophilus influenza was the most common bacterium (9.4%), followed by Klebsiella pneumoniae and Pseudomonas aeruginosa (with 4.0%). In infants (toddlers), ages 12 to 24 months with severe viral pneumonia, (odds ratio [OR] = 3.37, 95% confidence interval [CI]: 1.22–9.33), the higher concentrations of procalcitonin (PCT; OR = 1.16; 95% CI: 1.00–1.34), and neutrophils (OR = 1.13; 95% CI: 1.04–1.22) were associated with a higher risk of coinfection. This study underlined the pervasiveness of coinfections among young children with severe viral pneumonia. Provision of effective antiviral treatment, especially for RSV, as well as the advancement of sensitive and rapid diagnostic tools for screening pathogens of pneumonia, is critical to reducing the burden of this disease.
Methadone for Opioid Use Treatment during Pregnancy: Trends in Postpartum Dose Adjustments
Authors: Ward Clara , Christensen Carl W. 
Published: August 2020

AbstractObjective This study examines methadone dose adjustment postpartum.Methods A retrospective study of women with methadone for opioid use treatment (OUT) during pregnancy was performed. Patient charts were reviewed and data were extracted. Methadone doses from five temporal data points for each patient were used: starting dose, day of delivery, and 1, 2, and 6 months postpartum.Results Over 26 months, 49 pregnancies to women using methadone for OUT were evaluated and 20 (41%) were included. The mean methadone starting dose was 47 mg, compared with 86 mg at the time of delivery. The mean dose postpartum remained unchanged from delivery and 75% of pregnancies required the same dose or higher 1 month postpartum. By 2 months postpartum, only 33% were able to decrease their methadone dose. Twelve pregnancies completed follow-up until 6 months postpartum; only 17% of patients were able to decrease their dose, with an overall mean dose decrease was 12%. There was no difference between the mean dose at delivery and the 6-month postpartum dose.Conclusion Patients using methadone for OUT during pregnancy achieved minimal dose decreases postpartum. Patients should be counseled that postpartum dose tapers may be challenging and about alternatives to methadone for OUT.
Effectiveness of Progestogens as Maintenance Tocolysis and Urogenital Cultures: Secondary Analysis of the PROTECT Trial
Authors: Pignatti Lucrezia , D'Amico Roberto , Vergani Patrizia , Di Tommaso Mariarosaria , Acaia Barbara , Benedetto Chiara , Facchinetti Fabio 
Published: August 2020

Abstract
Background In a recently published multicenter randomized controlled trial, we demonstrated that progestogens are not effective as maintenance tocolysis.
Objective This study was aimed to evaluate if previous finding may be affected by positive urine culture and/or vaginal swab.
Study Design We performed a secondary analysis of the PROTECT trial (NCT01178788). Women with singleton pregnancy between 22 and 31 6/7 weeks' gestation, admitted for threatened preterm labor were considered. At admission, we collected urine culture and vaginal swabs. At discharge, women with a cervical length ≤25 mm were randomized to vaginal progesterone or 17α-hydroxyprogesterone caproate or observation group. We used Chi-square statistics, considering 97.5% CI (confidence interval) and p-value less than 0.025 for significance.
Results Urine culture and vaginal swabs were collected in 232 out of 235 patients included in the primary analysis. Overall, 31 out of 232 women (13.4%) had positive urine culture and 60 out of 232 (25.9%) had positive vaginal swab. In women with negative urine culture, a higher rate of preterm birth was found in vaginal progesterone group (27/69, 39.7%) respect with controls (14/68, 20.6%; relative risk [RR] = 1.90; 97.5% CI: 1.01–3.57; p = 0.018).
Conclusion Among women with negative urine culture, the rate of preterm birth <37 weeks' gestation was significantly increased in those receiving vaginal progesterone, reinforcing our previous findings in symptomatic women.