bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Natural Historian's Guide to the CT Galaxy: Step-by-Step Instructions for Preparing and Analyzing Computed Tomographic (CT) Data Using Cross-Platform, Open Access Software
Authors: Buser T J , Boyd O F , Cortés Á , Donatelli C M , Kolmann M A , Luparell J L , Pfeiffenberger J A , Sidlauskas B L , Summers A P 
Published: April 2020

Synopsis
The decreasing cost of acquiring computed tomographic (CT) data has fueled a global effort to digitize the anatomy of museum specimens. This effort has produced a wealth of open access digital three-dimensional (3D) models of anatomy available to anyone with access to the Internet. The potential applications of these data are broad, ranging from 3D printing for purely educational purposes to the development of highly advanced biomechanical models of anatomical structures. However, while virtually anyone can access these digital data, relatively few have the training to easily derive a desirable product (e.g., a 3D visualization of an anatomical structure) from them. Here, we present a workflow based on free, open source, cross-platform software for processing CT data. We provide step-by-step instructions that start with acquiring CT data from a new reconstruction or an open access repository, and progress through visualizing, measuring, landmarking, and constructing digital 3D models of anatomical structures. We also include instructions for digital dissection, data reduction, and exporting data for use in downstream applications such as 3D printing. Finally, we provide Supplementary Videos and workflows that demonstrate how the workflow facilitates five specific applications: measuring functional traits associated with feeding, digitally isolating anatomical structures, isolating regions of interest using semi-automated segmentation, collecting data with simple visual tools, and reducing file size and converting file type of a 3D model.
Reporting, recording, and communication of COVID-19 cases in workplace: data protection as a moving target
Authors: Shabani Mahsa , Goffin Tom , Mertes Heidi 
Published: April 2020

Abstract
In response to concerns related to privacy in the context of coronavirus disease 2019 (COVID-19), recently European and national Data Protection Authorities (DPAs) issued guidelines and recommendations addressing a variety of issues related to the processing of personal data for preventive purposes. One of the recurring questions in these guidelines is related to the rights and responsibilities of employers and employees in reporting, recording, and communicating COVID-19 cases in workplace. National DPAs in some cases adopted different approaches regarding duties in reporting and communicating the COVID-19 cases; however, they unanimously stressed the importance of adopting privacy-preserving approaches to avoid raising concerns about surveillance and stigmatization. We stress that in view of the increasing use of new data collection and sharing tools such as ‘tracing and warning’ apps, the associated privacy-related risks should be evaluated on an ongoing manner. In addition, the intricacies of different settings where such apps may be used should be taken into consideration when assessing the associated risks and benefits.
Observational constraints on the multiphase nature of outflows using large spectroscopic surveys at z ∼ 0
Authors: Roberts-Borsani G W 
Published: April 2020

ABSTRACT
Mass outflow rates and loading factors are typically used to infer the quenching potential of galactic-scale outflows. However, these generally rely on observations of a single gas phase that can severely underestimate the total ejected gas mass. To address this, we use observations of high mass (≥1010 M⊙), normal star-forming galaxies at z ∼ 0 from the MaNGA, xCOLD GASS, xGASS, and ALFALFA surveys and a stacking of Na d, Hα, CO(1–0), and H i 21 cm tracers with the aim of placing constraints on an average, total mass outflow rate, and loading factor. We find detections of outflows in both neutral and ionized gas tracers, with no detections in stacks of molecular or atomic gas emission. Modelling of the outflow components reveals velocities of |vNa d| = 131 km s−1 and |vHα| = 439 km s−1 and outflow rates of $\dot{M}_{\rm {Na\,\small{D}}}$ = 7.55 M⊙ yr−1 and $\dot{M}_{\text{H}\alpha }$ = 0.10 M⊙ yr−1 for neutral and ionized gas, respectively. Assuming a molecular/atomic outflow velocity of 200 km s−1, we derive upper limits of $\dot{M}_{\text{CO}}\lt 19.43$ M⊙ yr−1 and $\dot{M}_{\rm {H\,\small {I}}}\lt $ 26.72 M⊙ yr−1 for the molecular and atomic gas, respectively. Combining the detections and upper limits, we find average total outflow rates of $\dot{M}_{\text{tot}}\lesssim$27 M⊙ yr−1 and a loading factor of ηtot ≲ 6.39, with molecular gas likely contributing ≲72 per cent of the total mass outflow rate, and neutral and ionized gas contributing ∼28 and &lt;1 per cent, respectively. Our results suggest that, to first order, a degree of quenching via ejective feedback could occur in normal galaxies when considering all gas phases, even in the absence of an active galactic nucleus.
RNase AM, a 5′ to 3′ exonuclease, matures the 5′ end of all three ribosomal RNAs in E. coli
Authors: Jain Chaitanya 
Published: April 2020

AbstractBacterial ribosomal RNAs (rRNAs) are transcribed as precursors and require processing by Ribonucleases (RNases) to generate mature and functional rRNAs. Although the initial steps of rRNA processing in Escherichia coli (E. coli) were described several decades ago, the enzymes responsible for the final steps of 5S and 23S rRNA 5′-end maturation have remained unknown. Here, I show that RNase AM, a recently identified 5′ to 3′ exonuclease, performs the last step of 5S rRNA 5′-end maturation. RNase AM was also found to generate the mature 5′ end of 23S rRNA, subsequent to a newly identified prior processing step. Additionally, RNase AM was found to mature the 5′ end of 16S rRNA, a reaction previously attributed to RNase G. These findings indicate a major role for RNase AM in cellular RNA metabolism and establish a biological role for the first 5′ to 3′ RNA exonuclease identified in E. coli.
High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme
Authors: Lat Prince Kumar , Liu Kun , Kumar Dev N , Wong Kenneth K L , Verheyen Esther M , Sen Dipankar 
Published: April 2020

AbstractGuanine-rich, single-stranded DNAs and RNAs that fold to G-quadruplexes (GQs) are able to complex tightly with heme and display strongly enhanced peroxidase activity. Phenolic compounds are particularly good substrates for these oxidative DNAzymes and ribozymes; we recently showed that the use of biotin-tyramide as substrate can lead to efficient GQ self-biotinylation. Such biotinylated GQs are amenable to polymerase chain reaction amplification and should be useful for a relatively non-perturbative investigation of GQs as well as GQ–heme complexes within living cells. Here, we report that in mixed solutions of GQ and duplex DNA in vitro, GQ biotinylation is specifically &gt;104-fold that of the duplex, even in highly concentrated DNA gels; that a three-quartet GQ is tagged by up to four biotins, whose attachment occurs more or less uniformly along the GQ but doesn’t extend significantly into a duplex appended to the GQ. This self-biotinylation can be modulated or even abolished in the presence of strong GQ ligands that compete with heme. Finally, we report strong evidence for the successful use of this methodology for labeling DNA and RNA within live, freshly dissected Drosophila larval salivary glands.
Performance, heat tolerance response, and blood metabolites of water-restricted Xhosa goats supplemented with vitamin C1
Authors: Akinmoladun Oluwakamisi F , Fon Fabian N , Mpendulo Conference T , Okoh Omobola 
Published: April 2020

Abstract
Water restriction in small ruminants is usually accompanied by a drop in feed intake, body weight, and disturbances in the normal internal milieu. However, attempts to lessen the burden of water stress with vitamin C (VC) supplementation have been greeted with conflicting reports. Therefore, this experiment was conducted to evaluate the effect of single and/or multiple VC supplementations in water-restricted Xhosa goats by evaluating their performance, heat tolerance, and blood metabolites. In total, 42 does, 12 mo old and with an average weight of 15.92 ± 2.12 kg were evaluated for 75 d. The does were distributed according to a complete randomized design into seven groups of six comparable animals: W0, without water restriction (control); W70, water restriction of 70% of ad libitum water intake (WI); W50, water restriction of 50% ad libitum WI; W70+, water restriction of 70% of ad libitum WI plus 3 g VC daily; W50+, water restriction 50% of ad libitum WI plus 3 g VC daily; W70++, water restriction of 70% of ad libitum WI plus 3 g VC and extra 5 g VC given every eighth day; and W50++, water restriction of 50% of ad libitum WI plus 3 g VC and extra 5 g VC given every eighth day. Goats under the W50 group were the most affected (P &lt; 0.05) and the effect was more pronounced in their body condition scores (BCs). Weight loss due to water restriction was reduced by VC supplementation in treated groups. Changes in body thermal gradient, rectal temperature, cholesterol, and bilirubin were similar (P &gt; 0.05) across the various experimental groups. The attenuation effect of VC was significant (P &lt; 0.05) in responses to respiratory rate, Na+, K+, Mg2+, Cl−, Ca2+, and urea. Supplementation of VC (either single or multiple) did not significantly (P &gt; 0.05) improve the effect of water restriction on BCs, FAMACHA, glucose, globulin, alanine aminotransferase, and high-density lipoprotein. The additive effect of multiple VC significantly influenced (P &lt; 0.05) Na+ and Mg2+. Limited WI affects growth and other physiological parameters in Xhosa goats. However, supplementation of VC may be beneficial at modulating the stressful stimuli imposed by water stress.
New Liberalism and the City of London: Reassessing Empire, Finance, and Politics in Francis Hirst’s Economist, 1906–16
Authors: Zevin Alexander 
Published: April 2020

Abstract
The influence of the City of London on British politics has been a focus of controversy among historians. Likewise, the ‘death of liberal England’, during the years in which Liberals governed in the run-up to the First World War. The Economist, as the City’s leading liberal weekly, allows us to explore the connection between these themes, in ways that challenge scholarly assumptions about both. Under Francis Hirst, its editor and an influential New Liberal thinker in his own right, The Economist acted as a bridge between the realms of finance capital and political practice, at just the moment that a serious conflict appeared to divide them—over the new taxes and social reform measures in the People’s Budget of 1909–10. This article deploys Hirst and his tenure at The Economist—including his ejection in 1916 for supporting a negotiated peace during the First World War—to argue that finance and politics were deeply intertwined in liberal understandings of free trade, empire, and social reform by the turn of the twentieth century; in addition, it suggests that the conflicts that emerged at this time, over the interests of the City and how and if these were compatible with other economic, social, or political aims or actors, prefigured later, better-known clashes that have recurred in Britain down to the present.
Phyloanatomic characterization of the distinct T cell and monocyte contributions to the peripheral blood HIV population within the host
Authors: RifeMagalis Brittany , Strickland Samantha L , Shank Stephen D , Autissier Patrick , Schuetz Alexandra , Sithinamsuwan Pasiri , Lerdlum Sukalaya , Fletcher James L K , de Souza Mark , Ananworanich Jintanat , Valcour Victor ,   The Search007 Study Group, Williams Kenneth C , Kosakovsky Pond Sergei L , RattoKim Silvia , Salemi Marco 
Published: April 2020

AbstractHuman immunodeficiency virus (HIV) is a rapidly evolving virus, allowing its genetic sequence to act as a fingerprint for epidemiological processes among, as well as within, individual infected hosts. Though primarily infecting the CD4+ T-cell population, HIV can also be found in monocytes, an immune cell population that differs in several aspects from the canonical T-cell viral target. Using single genome viral sequencing and statistical phylogenetic inference, we investigated the viral RNA diversity and relative contribution of each of these immune cell types to the viral population within the peripheral blood. Results provide evidence of an increased prevalence of circulating monocytes harboring virus in individuals with high viral load in the absence of suppressive antiretroviral therapy. Bayesian phyloanatomic analysis of three of these individuals demonstrated a measurable role for these cells, but not the circulating T-cell population, as a source of cell-free virus in the plasma, supporting the hypothesis that these cells can act as an additional conduit of virus spread.
Productivity and Health: Physical Activity as a Measure of Effort
Authors: Akogun Oladele , Dillon Andrew , Friedman Jed , Prasann Ashesh , Serneels Pieter 
Published: April 2020

Abstract
This paper examines the relationship between physical activity and individual productivity among agricultural workers paid on a piece-rate basis. In the context studied, physical activity has a clear correspondence with worker effort. Agricultural workers’ physical activity is directly observed from accelerometer data and is robustly associated with their daily productivity. In addition the impact of a health intervention, which provides malaria testing and treatment, on physical activity and productivity, indicates that the increased daily productivity of workers who are offered this program is explained by worker effort reallocation from low-intensity to high-intensity work within a fixed time period. This demonstrates, in settings when individual productivity is observed, that physical activity measures can help disentangle productivity effects due to effort. When productivity is unobserved, physical activity measures may proxy for individual productivity in physically demanding tasks. The challenges and limitations of physical activity measurement using accelerometers is discussed including their potential use for alternative contexts and the importance of field and data analysis protocols.
Dromaeosaurid crania demonstrate the progressive loss of facial pneumaticity in coelurosaurian dinosaurs
Authors: Brownstein Chase Doran 
Published: April 2020

AbstractDinosaurs are notable for their extensive skeletal pneumaticity, a feature that may have helped facilitate the development of various ‘extreme’ body plans in this group. Despite its relevance to understanding the evolution of the avian body plan, this feature has only been described in detail for a few non-avian dinosaurs, and cranial pneumaticity outside the braincase remains poorly documented. I describe facial pneumatic features in members of the Dromaeosauridae, a clade of hypercarnivorous dinosaurs closely allied to birds. Variation in the pneumaticity of the nasals and jugals, the position and shape of the pneumatic fenestrae of the maxilla and the border of the antorbital fossa shows that facial pneumaticity differed substantially among closely related dromaeosaurids and other bird-like dinosaurs. Ancestral state reconstructions of facial pneumaticity in coelurosaurs suggest a complex evolutionary history for these features. Surprisingly, the general trend along the path towards birds was the loss or reduction of superficial pneumatic features on the snout and cheek. Some facial pneumatic features seem to have evolved secondarily in some derived bird-like forms. The results show superficial facial pneumaticity did not increase in coelurosaurs and emphasize the complexity of the evolution of pneumatization in the lineage leading to birds.
Quantifying Mobility in the ICU: Comparison of Electronic Health Record Documentation and Accelerometer-Based Sensors to Clinician-Annotated Video
Authors: Fazio Sarina , Doroy Amy , Da Marto Natalie , Taylor Sandra , Anderson Nicholas , Young Heather M. , Adams Jason Y. 
Published: April 2020

Objectives:
To compare the accuracy of electronic health record clinician documentation and accelerometer-based sensors with a gold standard dataset derived from clinician-annotated video to quantify early mobility activities in adult ICU patients.

Design:
Prospective, observational study.

Setting:
Medical ICU at an academic hospital.

Patients:
Adult ICU patients (n = 30) were each continuously monitored over a median of 24.4 hours, yielding 711.5 hours of video, electronic health record, and sensor data.

Interventions:
None.

Measurements and Main Results:
Electronic health record documentation estimated ambulation (intraclass correlation coefficient, 0.89; 95% CI, 0.78–0.95), sitting out-of-bed (intraclass correlation coefficient, 0.85; 95% CI, 0.72–0.93), and turning events (intraclass correlation coefficient, 0.87; 95% CI, 0.75–0.94) with excellent agreement but underestimated the number of standing, transferring, and pregait activities performed per patient. The accelerometer-based sensor had excellent agreement with video annotation for estimating duration of time spent supine (intraclass correlation coefficient, 0.99; CI, 0.97–0.99) and sitting/standing upright (intraclass correlation coefficient, 0.92; CI, 0.82–0.96) but overestimated ambulation time.

Conclusions:
Our results show that electronic health record documentation and sensor-based technologies accurately capture distinct but complimentary metrics for ICU mobility measurement. Innovations in artifact detection, standardization of clinically relevant mobility definitions, and electronic health record documentation enhancements may enable further use of these technologies to drive critical care research and technology leveraged data-driven ICU models of care.
Lumbopelvic motor control function between patients with chronic low back pain and healthy controls: a useful distinguishing tool
Authors: Jung Sung-hoon , Hwang Ui-jae , Ahn Sun-hee , Kim Hyun-a , Kim Jun-hee , Kwon Oh-yun 
Published: April 2020

Abstract
Although lumbopelvic stability exercise improves lumbopelvic motor control function in patients with chronic low back pain (CLBP), the difference in lumbopelvic motor control function between the patients with CLBP and the healthy controls is unclear. The purpose of this study was to compare lumbopelvic motor control function between patients with CLBP and healthy controls and to determine the prevalence of CLBP according to core stability function.
For this study, 278 participants were recruited, including patients with CLBP (n = 137) and healthy controls (n = 141). The participants performed a core stability function test and were classified to either the low or high core stability function group according to their core stability function for CLBP prevalence analysis.
Lumbopelvic motor control was significantly higher in the healthy controls than in patients with CLBP. Of the patients in the low lumbopelvic motor control function group, 65.9% had CLBP, whereas 36.8% of the patients in the high lumbopelvic motor control function group had CLBP. Lumbopelvic motor control function demonstrated a significant difference between the patients with CLBP and the healthy controls. The lumbopelvic motor control function test was demonstrated to be an effective diagnostic tool for distinguishing CLBP. This information can be applied in assessments and interventions for CLBP in clinical settings.
Three dimensional finite element analysis used to study the influence of the stress and strain of the operative and adjacent segments through different foraminnoplasty technique in the PELD
Authors: Xie YiZhou , Wang Xinling , Jian Qiang , Fan Xiaohong , Yu Yang , Gu Dangwei , Wu WeiDong 
Published: April 2020

Abstract

Introduction:
Percutaneous endoscopic lumbar disectomy (PELD) is one of the most popular minimally invasive techniques of spinal surgery in recent years. At present, there are 2 main surgical approaches in PELD: foraminal approach and interlaminar approach. What's more, foraminoplasty is a necessary step for both approaches. However, there are few biomechanical studies on the formation of different parts of the intervertebral foramen. The aim of this study is to explore the effects of different foraminoplasty methods on the biomechanics of the corresponding and adjacent segments of the lumbar through a 3-dimensional finite element model analysis.

Methods:
We established a normal 3-dimensional finite element mode of L3 to L5, simulated lumbar percutaneous endoscopy by doing cylindrical excision of bone whose diameter was 7.5 mm on the L5 superior articular process and the L4 inferior articular process, respectively, so that we obtained 3 models: the first one was normal lumbar model, the second one was the L4 inferior articular process shaped model, and the third one was the L5 superior articular process shaped model. We compared the biomechanics of the intervertebral disc of L3/4 and L4/5 when they were in the states of forward flexion, backward extension, left and right flexion, and left and right rotation on specific loading condition.

Discussion:
If the outcomes indicate the trial is feasible and there is evidence that one of the foraminoplasty technique may make few differences in biomechanics of corresponding lumbar intervertebral disc, we will proceed to a definitive trial to test the best way to foraminplasty, which could make biomechanical influence as little as possible.
Trial Registration: Chinese Clinical Trial Registry, ChiCTR1900026973. Registered on September 27, 2019.
The first reported case of Beaulieu-Boycott-Innes syndrome caused by two novel mutations in THOC6 gene in a Chinese infant
Authors: Zhang Qiang , Chen Shaoke , Qin Zailong , Zheng Haiyang , Fan Xin 
Published: April 2020

Abstract

Rationale:
This case report expands the mutation and phenotypic spectra of Beaulieu-Boycott-Innes syndrome (BBIS), and will be valuable for mutation-based pre- and post-natal screening of BBIS when conducting a genetic diagnosis.

Patient concerns:
A 4-year old boy from Guilin City, Guangxi Zhuang Autonomous Region, China, was referred to our clinic for clarification of his diagnosis because he showed moderate intellectual disability.

Diagnosis:
Two novel compound heterozygous mutations of THOC6, c.664T>C (p.Trp222Arg) and c.945+1 G>A were identified in this patient by whole exome sequencing. The two mutations were evaluated as pathogenic and likely pathogenic respectively according to the American College of Medical Genetics guidelines. This is the first case displaying the BBIS phenotype reported in the Chinese population. These two mutations have not been reported previously.

Interventions:
Symptomatic treatment and rehabilitation training for patients.

Outcomes:
The genetic cause of the disease was identified. The family received scientific genetic counseling.

Lessons:
BBIS is a rare syndromic autosomal recessive disease with intellectual disability and it is normally difficult for clinicians to recognize it. Whole exome sequencing is an efficient way to identify the gene which causes a particular disease in patients.