bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Wuchereria bancrofti infection in human and vector populations from Center-East, East and South-West regions of Burkina Faso: preliminary results
Authors: Coulibaly Sanata , Hien Aristide Sawdetouo , Bamogo Rabila , Some Fabrice A. , Diabaté Abdoulaye , Sangaré Ibrahim , Ouedraogo Georges Anicet , Fournet Florence , Bougouma Clarisse , Bougma Roland Windaré , Koudou Benjamin B. , Dabire Roch Kounbobr 
Published: July 2020

Abstract
Background

To eliminate lymphatic filariasis (LF), since 2001, Burkina Faso adopted a community-based treatment with annual mass drug administration (MDA) of ivermectin-abendazole. However, after more than 11 rounds of MDA, it remained some hotpots where transmission is still being active. Therefore, to better scale up elimination measures toward 2030, it seems necessary to assess the prevalence of Wuchereria bancrofti both in human and vector populations.
Methods

Parasitological and entomological surveys were conducted in August 2014 and in September 2015, in six hotpots areas dispersed in Centre-East, East and South-West health regions for blood and vector’s collections. Nocturnal finger-prick blood samples and microscopic observations were performed to determine the prevalence of W. bancrofti in humans. Mosquitoes were collected by human landing catches and indoor spraying collections. They were identified morphologically and those of the Anopheles gambiae s.l. were confirmed by PCR. Then the W. bancrcofti prevalence within such vector populations was determined by conventional and LAMP PCR technique using the DNA of pooled mosquitoes grouped by species.
Results

The results highlighted the persistence of W. bancrofti infection both in human and vector populations after multiple rounds of MDA in the sites. The prevalence of W. bancrofti in human populations was reduced in 2015 compared to 2014. Within the An. gambiae s.l., only An. coluzzii was found infected by W. bancrofti respectively in Centre-East and East health regions, whereas An. nili was the most infected vector in Saptan in the South-West health region. Some specimens of An. funestus s.l. were tested positive to W. bancrofti with the LAMP PCR but failed to be confirmed by the conventional technique.
Conclusions

Despite the MDA campaigns supplied by vector control interventions relied on the mass coverage of insecticide treated nets, the incidence of LF is still effective in these hotspots but luckily in decreasing trend. The novelty is that An. nili was found infected by W. bancrofti that increased the number of potential vectors of W. bancfrofti in Burkina Faso.
A cross-sectional study of opioid involvement in non-poisoning suicide – risks and prevention opportunities
Authors: Mason Maryann , McLone Suzanne , Bartell Tami , Welch Sarah , Sheehan Karen , Post Lori 
Published: July 2020

Abstract
Background

The current opioid epidemic has drawn attention to drug overdose deaths including unintentional and suicide poisoning deaths which peaked in the United States in 2017. Concurrent with the opioid epidemic, the number and rate of suicides in the United States has increased. At the same time, the proportion of suicide deaths across cause of death has shifted and the proportion of suicides by poisoning (including overdose) has decreased. On the face of it, it would appear that the opioid epidemic has not intersected with suicide as signaled by the decline in suicide deaths due to poisoning. However, opioid use and misuse is associated with suicidal ideation and attempts and therefore it is plausible that opioids may play a role in suicide deaths by causes other than poisoning.
Objective

This study examines opioid involvement (as measured by the presence of opioids but below the lethality threshold) in suicides by causes other than poisoning,
Methods

A cross-sectional study utilizing Illinois National Violent Death Reporting System data including all suicides toxicology screened for opioids. Chi-square tests were used to compare decedent and incident circumstance characteristics by opioid toxicology screen status.
Results

Of 1007 non-poisoning suicides screened for opioids, 83.6% (842) were opioid negative and 16.4% (165) were opioid positive. Over half (52.7%) of decedents positive for opioids died by firearm. White race, age 75 and over, and widowed or unknown marital status were associated with opioid positivity. Opioid positivity is linked to testing positive for other substances. One quarter of decedents testing positive for opioids had a history of substance abuse. Twenty eight percent of opioid positive decedents suffered from physical health problems.
Conclusion

Suicide decedents who are opioid positive and who die from causes other than poisoning have distinct characteristics which suggest an array of suicide prevention efforts – for example -- including information on risk of suicide for opiate users in firearm sales, including suicide prevention counseling in health care settings in which opiates and/or benzodiazepines are therapeutically prescribed, and close monitoring of pain symptoms among patients experiencing chronic pain. ​
WITHDRAWN: Development of the “COVID-19 Psychological Destruction Scale”: A Validity and Reliability Study
Authors: Akan Yunus 
Published: July 2020

Abstract
This research is a scale development study to determine the level of destruction that COVID-19 has created on the psychology of individuals in the 18-50 age group. Data were collected by convenience sampling method, which allowed us to reach total 1604 participants, aged between 18 and 50 years, from 7 regions in Turkey. At the end of exploratory factor analysis performed on a 60-item pilot form, a two-factor structure was obtained explaining 61.74% of the total variance. The “Collapse” dimension has 14 items and the “Fear” dimension has 4 items. Load values of the items in the “Collapse” dimension are between 0.700 - 0.855 and the load values of items in the "Fear" dimension are between 0.736 - 0.797. While the reliability analysis showed a Cronbach's Alpha value of 0.951 for the whole scale, the Cronbach's Alpha values for the dimensions were 0.937 and 0.791, respectively. A survey was implemented with 597 new participants for convergent and discriminant validity, and the Pearson Moments Product Correlation coefficient between the developed “COVID-19 Psychological Destruction Scale” and “Depression Anxiety Stress Scale (DASS)” was found to be 0.748. A confirmatory factor analysis was performed on the new data set with 597 participants and the goodness of fit index values obtained showed that the model perfectly fit. The result of the research reveals that the “COVID-19 Psychological Destruction Scale” is a valid and reliable scale that can be applied to individuals aged 18-50.
Whole Body Positron Emission Tomography Attenuation Correction Map Synthesizing using 3D Deep Generative Adversarial Networks
Authors: Colmeiro Ramiro Rodriguez , Verrastro Claudio , Minsky Daniel , Grosges Thomas 
Published: July 2020

Abstract
Background: The correction of attenuation effects in Positron Emission Tomography (PET) imaging is fundamental to obtain a correct radiotracer distribution. However direct measurement of this attenuation map is not error-free and normally results in additional ionization radiation dose to the patient. Here, we propose to obtain the whole body attenuation map using a 3D U-Net generative adversarial network. The network is trained to learn the mapping from non attenuation corrected 18-F-fluorodeoxyglucose PET images to a synthetic Computerized Tomography (sCT) and also to label the input voxel tissue. The sCT image is further refined using an adversarial training scheme to recover higher frequency details and lost structures using context information. This work is trained and tested on public available datasets, containing several PET images from different scanners with different radiotracer administration and reconstruction modalities. The network is trained with 108 samples and validated on 10 samples.Results: The sCT generation was tested on 133 samples from 8 distinct datasets. The resulting mean absolute error of the network is 103 ± 18 HU and a peak signal to noise ratio of 18.6 ± 1.5 dB. The generated images show good correlation with the unknown structural information.Conclusions: The proposed deep learning topology is capable of generating whole body attenuation maps from uncorrected PET image data. Moreover, the method accuracy holds in the presence of data form multiple sources and modalities and is trained on publicly available datasets.
The Relationship between Energy-Adjusted Dietary Inflammatory Index (E-DII) with Quality of Life and Inflammatory Markers among Overweight and Obese Iranian Women
Authors: Ghodoosi Nasim , Arghavani Hana , Mirzababaei Atieh , Yekaninejad Mir Saeed , Keshavarz Seyed Ali , Imani Hossein , mirzaei Khadijeh - 
Published: July 2020

Abstract
Background

Overweight and obesity are associated with higher levels of inflammatory markers, inflammatory diseases, and lower quality of life. Recent investigations have shown that diet can modify these complications and improve quality of life.
Objective

The objective of this study was to investigate associations between Energy-Adjusted Dietary Inflammatory Index (EDII) with quality of life and serum inflammatory markers.
Methods

In the current cross-sectional study, a total of 280 females from health centers in Tehran, Iran (2018) were selected for the study using a multistage cluster random sampling method. The EDII was computed based on dietary intake, assessed by a previously validated 147-item food frequency questionnaire (FFQ). Before calculating EDII, a residual method was used to adjust energy intake. SF-36 questionnaires were used for evaluating quality of life. Concentrations of high-sensitivity C-reactive protein (hs-CRP) were determined using collected serum samples.
Results

The mean age of the study participants was 36.30 ± 8.05 years. Analyses were performed using multivariable linear regression, adjusting for age, weight, physical activity, smoking status, economic status and employment status. Linear regression analysis demonstrated that E-DII were significantly associated with certain quality of life criteria, such as physical function, mental health, and vitality (β = 5.58, 95% CI 0.72, 10.43, p = 0.024, β = 16.88, 95% CI 10.75, 23, p < 0.0001 and β = 14.29, 95% CI 9.48, 20.36, p < 0.0001, respectively). No significant associations were observed between EDII and serum level hs-CRP.
Conclusion

It was found that dietary inflammatory potential decreased some quality of life measurements and levels in overweight and obese Iranian women.
Serum Level of microRNA-429 is as a Promising Diagnostic Biomarker for Cervical Cancer Patients
Authors: Zhang Keqian , Mao Tianqi , He Zhicheng , Wu Xiaojiao , Peng Yu , Chen Yanrong , Dong Yan , Ruan Zhihua , Wang Zhe 
Published: July 2020

Abstract
Backgrounds: Serum microRNAs (miRNAs) are a novel class of diagnostic biomarkers for human cancers. However, the clinical relevance of circulating microRNA-429 (miR-429) in cervical cancer patients remains unclear. The purpose of this study was to investigate serum miR-429 levels in cervical cancer patients, as well as its association with clinical characteristics and diagnostic value.Methods: Serum miR-429 expression was investigated in 113 cervical cancer patients and 107 healthy normal controls by quantitative real-time PCR (qRT-PCR). Chi-square test was applied to evaluate the correlation between miR-429 expression and clinicopathological parameters. Receiver operating characteristic (ROC) curves were constructed to assess the diagnostic significance of serum miR-429 in cervical cancer.Results: Serum miR-429 expression was significantly down-regulated in cervical cancer compared with healthy controls (P=0.000). In addition, serum miR-429 level was correlated with differentiation (P=0.031), FIGO stage (P=0.030), and lymph node metastasis (P=0.045). ROC curves demonstrated that serum miR-429 expression could distinguish cervical cancer patients from healthy controls with the sensitivity of 81.4% and the specificity of 69.2%. Additionally, the area under the curve (AUC) was 0.816 and the cut-off value was 0.825.Conclusion: MiR-429 is down-regulated in cervical cancer and associated with aggressive clinical characteristics. MiR-429 may be a potential diagnostic biomarker for cervical cancer.
Mechanism of Astragalus Membranaceus in the Treatment of Diabetic Nephropathy Based on Network Pharmacology
Authors: an Xuedong , Jin De , Duan LiYun , Zhao Yiru , Zhao Shenghui , Zhou RongRong , Duan YingYing , Lian Fengmei , Tong Xiaolin 
Published: July 2020

Abstract
Background: Diabetic nephropathy (DN), which affects more than 40% of diabetic patients, is still the main cause of end-stage renal disease in most countries. Prescription containing Astragalus Membranaceus (AM) in the treatment of early stage DN with significant effect, but the mechanism is unclear, which we would explore based on network pharmacology.Method: First, we searched the database of China National Knowledge Internet, Wanfang Database, Chinese Biomedical Literature Database, PubMed, EMBASE, Cochrane database about the randomized, single (double) blind, controlled clinical studies of prescription containing AM in the treatment of DN, determined the effectiveness of prescription containing AM in the treatment of DN. Then, the effective components of AM in Traditional Chinese Medicine systems taxonomy database and analysis platform (TCMSP), Traditional Chinese medicine integrated database (TCMID) and Bioinformatics analysis tool for molecular mechanism of traditio Internal Chinese medicine (BATMAN-TCM) database were searched. According to the oral utilization ≥ 30% and drug-like ≥0.18, the effective components were screened. PubChem and health information technology (HIT) databases were searched for query validation targets and Simplified molecular input line entry specification (SMILES) of the effective ingredients, and Swisstarget prediction database was used to obtain prediction targets (possibility > 0). Drugbank, transient triple differential (TTD), and DisGeNET were searched for the targets related to DN. Finally, the data were integrated by the Cytoscape software, the drug-target-disease network was drawn, the protein interaction network was drawn with String database, and the signal pathway enrichment analysis was carried out with ClueGO.Result: The results showed that prescription containing AM could effectively reduce the urinary protein excretion rate [95% md-43.30 (- 57.00, - 29.61)]. 51 active components, 396 verified targets and 2330 predicted targets were searched. A total of 120 DN related targets were retrieved. 21 targets were found with Cytoscape. The main pathways were Interleukin-4 and 13 signaling, Activation of Matrix Metalloproteinases, EPH-Ephrin signaling.Conclusion: Prescription containing AM could effectively reduce the urinary protein excretion rate of DN patients. We speculated that AM mainly treated DN by regulating Angiotensin-converting enzyme (ACE), Vascular endothelial growth factor A (VEGFA), Janus Kinase 1 (JAK1), and interleukin-4 (IL-4) and interleukin-13 (IL-13) signaling, activation of matrix metalloproteins, ephrin signaling and other signaling pathways based on network pharmacology, which would be verified by animal experiments or in vitro experiments in the later.
Optogenetic Stimulation of The Motor Cortex Alleviates Neuropathic Pain in Rats of Infraorbital Nerve Injury With/Without CGRP Knock-Down
Authors: Islam Jaisan , KC Elina , Oh Byeong Ho , Kim Soochong , Hyun Sang-hwan , Park Young Seok 
Published: July 2020

Abstract
Background: Previous studies have reported that electrical stimulation of the motor cortex is effective in reducing trigeminal neuropathic pain; however, the effects of optical motor cortex stimulation remain unclear. Objective: The present study aimed to investigate whether optical stimulation of the primary motor cortex can modulate chronic neuropathic pain in rats with infraorbital nerve constriction injury.Methods: Animals were randomly divided into a trigeminal neuralgia group, a sham group, and a control group. Trigeminal neuropathic pain was generated via constriction of the infraorbital nerve and animals were treated via selective inhibition of calcitonin gene-related peptide in the trigeminal ganglion. We assessed alterations in behavioral responses in the pre-stimulation, stimulation, and post-stimulation conditions. In vivo extracellular recordings were obtained from the ventral posteromedial nucleus of the thalamus, and viral and α-CGRP expression were investigated in the primary motor cortex and trigeminal ganglion, respectively.Results: We found that optogenetic stimulation significantly improved pain behaviors in the trigeminal neuralgia animals and it provided more significant improvement with inhibited α-CGRP state than active α-CGRP state. Electrophysiological recordings revealed decreases in abnormal thalamic firing during the stimulation-on condition.Conclusion: Our findings suggest that optical motor cortex stimulation can alleviate pain behaviors in a rat model of trigeminal neuropathic pain. Transmission of trigeminal pain signals can be modulated via knock-down of α-CGRP and optical motor cortex stimulation.
Evaluation of Near Infrared Spectroscopy for Sporozoite Detection in Mosquitoes Infected With Wild-strain Parasites From Asymptomatic Gametocyte Carriers in Kilifi Kenya
Authors: Maia Marta Ferreira , Wagah Martin G. , Karisa Jonathan , Mwakesi Robert , Mure Festus , Muturi Martha , Wambua Juliana , Hamaluba Mainga , Dowell Floyd E. , Bejon Philip , Kapulu Melissa C. 
Published: July 2020

Abstract
Background: Screening for Plasmodium spp. sporozoite infection in mosquitoes is routinely done using ELISA (enzyme-linked immunosorbent assay). Near infrared spectroscopy (NIRS), a fast and non-destructive method, has recently been shown to distinguish, with 95% accuracy, between uninfected and sporozoite-infected mosquitoes using laboratory strains of Plasmodium falciparum (PfN54). The aim of this present study was to further investigate the reproducibility of NIRS to identify sporozoite infection in mosquitoes infected using field isolates of P. falciparum gametocytes from asymptomatic carriers. Methods: Healthy individuals (aged 5 years and above) were screened for gametocytaemia by thick-smear microscopy in an area of moderate transmission along the Coast of Kenya between May and September 2018. Asymptomatic gametocyte carriers were recruited for mosquito feeding assays, direct membrane feeding (DMFA) and direct skin feeding (DFA), using insectary-reared Anopheles gambiae s.s (Kilifi strain). Mosquitoes were kept for 14-days post feeding after which they were scanned using NIRS and subsequently analysed for sporozoite infection using circumsporozoite-ELISA. Predictive models were explored using partial least square regressions (PLS).Results: Two hundred and ninety-nine (299) individuals were screened for malaria parasites, 74 (24.8%) were found with circulating asexual parasites, and 16 (5.4%) with P. falciparum gametocyte stages.Fourteen (14) asymptomatic gametocyte carriers were recruited to the study for mosquito feeding assays. A total of 134 (7%, 134/1881) sporozoite-infected mosquitoes were obtained from 9 successful experiments. Three different training datasets composed of infected and uninfected mosquitoes were analysed. The PLS models were unable to distinguish between sporozoite-infected and uninfected mosquitoes. A predictive model could not be generated.Conclusions: The results of this study were not consistent with previous published research on NIRS for detection of sporozoite infection in the same mosquito species and may reflect differences between laboratory and field conditions. The current findings indicate that methods for sporozoite detection should be tested on field isolates at an early stage in their development and are informative for future research seeking novel high-throughput methods for parasite detection in mosquitoes.
Production of economic statistics for tourist accommodation services of Belo Horizonte under the flexible geography approach
Authors: César Soares Gonçalves Caio 
Published: July 2020

This paper aims to produce an experimental economic statistic for the tourist accommodation services for Belo Horizonte under a flexible geography perspective. The starting point is the definition of economic activities in the tourist accommodation concept. The methodology adopted is up-down divided into four steps including filtering processes to accompany the definition, discounts related to obtaining information only for the tourists and procedures for adjustments to the statistics always being compared to the official disclosures and respecting the sum of regions. This analysis revealed an erratic behaviour of the number of establishments in Belo Horizonte across the years and half of the workforce is concentrated in micro and small enterprises. The data from the perspective of the flexible geography allowed to verify where the almost R$ 86 million indicated by the value added of the tourist accommodations were located in 2015.
Keywords: Economic Statistics. Economic Geography. Accommodation Services. Flexible Geography.
In-vitro Bactericidal Activity of a Novel Plant Source Plumeria pudica against Some Human and Fish Pathogenic Bacteria
Authors: Das Shubhaisi , Burman Sunanda , Chandra Goutam 
Published: July 2020

Background:
The only remedy for up surging problem of antibiotic resistance is the
discovery of antibacterial agents of natural origin.

Objective:
The present study was aimed at finding antibacterial potential of crude and solvent extracts
of mature leaves of Plumeria pudica.

Methods:
Antibacterial activity of three different solvent extracts were evaluated in four human
and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum
Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics
were used as positive control. Preliminary phytochemical screening of most effective extract i.e.,
ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer
Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments
were done thrice and analyzed statistically.

Results:
Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all
selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with
maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts
indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed
the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional
groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of
TLC fraction of ethyl acetate extract, five different bioactive compounds e.g., 2,4-ditert –butylphenyl
5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-
2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified.

Conclusion:
Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.