bluuebunny/crossref_metadata_2025_split huggingface dataset:
Telepathology Practice in Cancer Diagnosis in Saint Jean de Dieu Hospital - Tanguieta, Benin
Authors: Azakpa Assogba Léopold , Priuli Friar Florent , Ndayake Essodina , Ganhouingnon Eric , Gonzalez-Rodilla Irène , Tchaou Meheza Parfait , Zanin Tiziano 
Published: October 2020

Context.—
Both the incidence of cancer and cancer-related mortality rates are high in sub-Saharan Africa, while resources for diagnosis and management are inadequate. In Benin, there is an extreme shortage of pathology services. Because of this shortage we built a histopathology laboratory equipped with an automated immunohistochemistry and a whole-slide imaging and telepathology system.

Objective.—
To report our experience of telepathology practice in the improvement of cancer diagnosis.

Design.—
The study was performed in our histopathology laboratory from January 1, 2016, to December 31, 2018. Resident laboratory technicians were trained in the preparation of microscopic and virtual slides by European pathologists. Virtual slides were stored on a Web-accessible server area for reading by 21 telepathologists in Benin and Europe. All patients with a histologic diagnosis of cancer were included in this study. Demographic data of patients, anatomic site of cancer, its histologic type, and its histologic grade were recorded.

Results.—
We registered 399 patients diagnosed with cancer of 1593 patients whose surgical specimens had been analyzed. There were 349 adults including 160 males and 189 females, and 50 children (both sexes) with a mean age of 53.40 years, 46.92 years, and 9.72 years, respectively. Eighty-three of 211 females (39.34%) had infiltrating breast carcinoma, and 34 of 188 males (18.09%) had prostatic carcinoma. Infiltrating carcinoma of no special type represented 51 (91.07%) of all infiltrating breast carcinomas. Prostatic carcinoma and infiltrating breast carcinoma were of high grade in 13 of 23 males (56.52%) and 34 of 56 females (60.71%), respectively.

Conclusions.—
Telepathology is enabling a great improvement in cancer diagnosis in our hospital.
'Override'-refleksiver i dansk og engelsk
Authors: Sørensen Sara , Ehlers Katrine Rosendal , Vikner Sten 
Published: October 2020

‘Override’ reflexives in Danish and English Surprisingly, English allows
(i) Peter’s behaviour only damages himself.(ii) Peter doesn’t realise that such behaviour only damages himself.
although himself does not refer to the subject (i.e. Peter’s behaviour/such behaviour). Far from all clauses allow himself to not refer to the subject, and therefore himself in (i-ii) is often called an ‘override’ reflexive. We suggest that ‘override’ reflexives are the combination of nonreflexive him with the intensifier himself, and that this is then ‘shortened’ from him himself to himself. In Danish, the combination of the corresponding pronoun ham with the corresponding intensifier selv results in the Danish ‘override’ reflexive ham selv. This paper will show how Danish having two versions of ham selv and English two versions of himself fits into the general reflexive systems of the two languages. The analysis will be held up against data from the major corpora: KorpusDK, BNC and COCA.
Punição física em casa e reprovação escolar relacionadas ao bullying
Authors: Zequinão Marcela Almeida , Oliveira Wanderlei Abadio de , Medeiros Pâmella de , Cidade Paola , Pereira Beatriz , Cardoso Fernando Luiz 
Published: October 2020

Backgroung: Several situations can affect the development and health of school children and adolescents. Bullying, characterized by a set of aggressive, intentional and repetitive behaviors, marked by the imbalance of power between victims and bullies, is one of the most serious problems in this sense, as it occurs in a context in which security and sociability are assumed. Therefore, this study was developed to expand the understanding about this phenomenon,contemplating variables ignored in many studies (family interactions, modes of discipline and school failure).
Objective: To analyze the participation of students in bullying situations and their experiences of vulnerability “to be beaten at home” and “grade retention”.

Methods: the study consisted of 409 students from the 3rd to the 7th grades of Brazilian Elementary Education of two public schools. The data were collected through a questionnaire instrument with structured questions with the purpose of describing the possible roles of participation in bullying and characterizing the students regardingthe context of social vulnerability in which they were. Vulnerability indicators were considered: per capita income, schooling of the adult population, housing conditions and crime / violence indices. Two schools were the research scenario. Data were analyzed using inferential statistics using the Chi-square test to verify the associationbetween the variables, Mann-Whitney U and Kruskal Wallis for comparison between groups.

Results: to be beaten at home was associated with the students’ involvement in bullying situations as victims, bullies and bully-victims. These students also differed in relation to years of grade retention and frequency of physical punishment when compared to students not involved in bullying situations. Students classified as bullyvictimsdemonstrated greater vulnerability in relation to the variables investigated. There were no significant differences in the comparison between participants’ sex and involvement in bullying situations.
Conclusion: It was observed that physical punishment used as a home discipline strategy and school failure are factors that increase students’ vulnerability to bullying or victimization. The data indicate that it is necessary to include families in anti-bullying intervention actions. At the same time, it must be considered that this inclusion should not be restricted to the field of education or school. It is necessary to think in an intersectoral way, especially including thefamily health teams that have moments with the families and that can be used to think about discipline techniques and how children, children and adolescents are disciplined at home.
Fabricating Perovskite Films for Solar Modules from Small to Large Scale
Authors: Yin Ruiyang , Wu Yuetong , Huang Zijian , Vasenko Andrey S. , Xu Shuoyang , Zhou Huanping 
Published: February 2025

AbstractPerovskite solar cells (PSCs) have gained significant attention for their high efficiency, low cost, and versatile application possibilities, which are expected to play a critical role in shaping the future of photovoltaics (PV) markets. However, the power conversion efficiency (PCE) and stability of large‐area PSCs still cannot meet the industrialization requirements, which are mainly associated with the unsatisfactory quality of large‐area perovskite films. This review first identifies the factors contributing to the film quality difference between small‐area and large‐area perovskite films, such as the solvent evaporation process, reaction and crystallization kinetics, etc. This results in undesired film quality for large‐area perovskites, e.g. film inhomogeneity in terms of morphology, composition, phase, crystal size, and orientation. Solvent systems are customed for different scalable preparation process based on their volatility, solubility, and coordination ability with perovskite. Furthermore, various additives are incorporated to further regulate surface tension change and intermediate phase evolution. Finally, we transition from the perovskite film level to the device level to explore the current advancements and challenges related to PCE and stability in the commercialization process.
Superior Thermally Stable Giant Piezomagnetism in Spin Frustrated Mn3Ir
Authors: Zhang Kaiqi , Ma Zhijie , Sun Ying , Shi Kewen , Wang Yuyan , Sun Qisong , Li YongJie , Deng Sihao , Cui Jin , Xia Zhengcai , Zhao Weisheng , Wang Cong 
Published: February 2025

AbstractThe piezomagnetic effect driven by magnetic‐strain coupling effectively generates and controls magnetization, making it pivotal for antiferromagnetic spintronics, a rapidly growing research field. However, the practical application of piezomagnetism to realize a large and stable piezomagnetic response over a wide temperature range remains a significant challenge due to the limited efficiency of magnetic‐strain coupling and thermal failure at room‐temperature. Here, the giant piezomagnetic effect in frustrated antiferromagnetic Mn₃Ir is reported for the first time, characterized by superior thermal stability and an exceptionally high Néel temperature of 950 K. The piezomagnetic coefficient of Mn3Ir reaches 0.55 µB f.u.−1 (1%), exceeding those of conventional antiferromagnetic piezomagnetic materials by an order of magnitude. Moreover, the direction of the piezomagnetic moment can be efficiently manipulated by the driving strain. The piezomagnetic effect, stable over a wide temperature range from 75 to 553 K, is attributed to a synergistic compensatory effect between the strains arising from lattice mismatch and temperature variation. These results provide a new paradigm for achieving thermally stable piezomagnetism and effectively manipulating antiferromagnetic states in variable‐temperature environments, thereby advancing spin manipulation in antiferromagnetic piezospintronics.
Branch Chain Variations Modulate Pyridine Derivative Adsorption for Long‐Life Zinc‐Ion Battery
Authors: Xu Lei , Xue Shiyan , Jia Xinwang , Liu Renke , Wang Longyang , Tao Li , Yao Jia , Zhang Jun , Wan Houzhao , Wang Yi , Wang Hao 
Published: February 2025

AbstractAqueous zinc‐ion batteries (AZIBs), candidates for large‐scale energy storage, face limitations due to the poor reversibility of zinc anodes. It reports on pyridine derivatives with high donor characteristics, including 2‐chloro‐1‐methylpyridinium iodide (CMPI) and pyridine‐2‐acetaldoxime methyl iodide (PAMI), as effective additives. At lower concentrations, these additives markedly curtail the zinc dendrites formation and the evolution of hydrogen on the zinc anode, thereby prolonging the AZIBs life. Through a combination of theory and experiments, the impact of side‐chain groups on the kinetic process of zinc depositioni is elucidated. In contrast to PAM+, CMPI+ demonstrates enhanced adsorption and self‐assembles at the anode‐electrolyte interface, forming a barrier to free water and a protective ZnI layer via I− ion integration. This dual‐layer strategy boosts zinc plating/stripping reversibility by 100‐fold and achieves a coulombic efficiency of 99.7% in zinc–copper half‐ batteries. The findings advance the understanding of electrolyte additive structures on zinc deposition, providing a molecular framework for screening additives in aqueous metal‐ion batteries.
A Novel Mesoporous Aluminum‐Based MOF with Large Pore Volume for High Concentration Benzene Adsorption
Authors: Hu Laigang , Zhang Mengxue , Wang Weiwei , Hu Jinru , Wu Wenhao , Lin Daohui , Yang Kun 
Published: February 2025

AbstractDeveloping large pore volume mesoporous metal‐organic frameworks (MOFs) is a reliable strategy for adsorbing high‐concentration benzene in the event of accidental leakage. Herein, a novel mesoporous Al‐based MOF, named ZJU‐928(Al), is synthesized using biphenyl‐3,4′,5‐tricarboxylic acid ligands and low toxic AlO6 cluster. It has larger pore volume (1.05 cm3 g−1), order hexagonal channel (32.10 Å), and higher specific surface area (2344 m2 g−1). As relative pressure up to 0.10, benzene adsorption of ZJU‐928(Al) rises to 9.99 mmol g−1, exhibiting an excellent saturation benzene adsorption of 11.37 mmol g−1 at 298 K. The isosteric heat of adsorption for benzene on ZJU‐928(Al) is only 24.52 kJ mol−1 at near‐zero loading, lower than that of most reported MOFs, allowing for lower energy consumption during ZJU‐928(Al) regeneration. ZJU‐928(Al) has excellent cyclical benzene adsorption‐desorption performance, and highest benzene diffusivity coefficient (2.65 × 10−5 cm2 s−1). Simulation shows that the excellent saturation benzene adsorption performance of ZJU‐928(Al) can be attributed to the larger pore volume accommodating more benzene molecules. Consequently, this research synthesizes a novel mesoporous Al‐based MOF with excellent saturation benzene adsorption, highlighting the potential of MOFs for high‐concentration toxic gas adsorption.
Directional Mushroom‐Derived Scaffold for Microenvironment Regulation in Infected Bone Defects
Authors: Yang Ganghua , Pan Hao , Wei Yuxuan , Yang Jianqiu , Zhang Zihan , Chen Shixuan , Wan Wenbing 
Published: February 2025

AbstractInfected bone defects are a common clinical condition, but conventional treatments often fail to achieve the desired outcomes, including addressing antibiotic resistance and preventing nonunion complications. In the presented study, a functionalized decellularized mushroom stem scaffold is developed composed of its naturally aligned channels, Zn2+/curcumin MOFs, hydroxyapatite minerals, and icariin. In vitro, It is found that functionalized acellular mushroom stem scaffold can control bacterial infections through Zn2+/curcumin MOFs. The naturally aligned channels guide bone mesenchymal stem cells (BMSCs) migration, and the components adsorbed on the acellular substrate further promote the migration of BMSCs. Moreover, these functional components further accelerated the polarization of M2 macrophage and osteogenic differentiation of BMSCs. In vivo, the functionalized decellularized mushroom stem scaffold cleared infected bacteria within 3 days, induced extracellular matrix secretion and alignment, and promoted new bone formation to cover defects within 8 weeks. The functionalized decellularized mushroom stem scaffold provides a promising strategy for treating infectious bone defects.
Double Confinement Design to Access Highly Stable Intermetallic Nanoparticles for Fuel Cells
Authors: Tian Lin , Gao Xiaoping , Zhu Mengzhao , Huang Zixiang , Wu Bei , Chen Cai , Ma Xianhui , Ruan Yaner , Guo Wenxin , Meng Xiangmin , Wang Huijuan , Du Wubin , He Shengnan , Pan Hongge , Zheng Xusheng , Wu Zhijun , Zhou Huang , Xia Jing , Wu Yuen 
Published: February 2025

AbstractMaintaining the stability of low Pt catalysts during prolonged operation of proton exchange membrane fuel cells (PEMFCs) remains a substantial challenge. Here, a double confinement design is presented to significantly improve the stability of intermetallic nanoparticles while maintaining their high catalytic activity toward PEMFCs. First, a carbon shell is coated on the surface of nanoparticles to form carbon confinement. Second, O2 is introduced during the annealing process to selectively etch the carbon shell to expose the active surface, and to induce the segregation of surface transition metals to form Pt‐skin confinement. Overall, the intermetallic nanoparticles are protected by carbon confinement and Pt‐skin confinement to withstand the harsh environment of PEMFCs. Typically, the double confined Pt1Co1 catalyst exhibits an exceptional mass activity of 1.45 A mgPt−1 at 0.9 V in PEMFCs tests, with only a 17.3% decay after 30 000 cycles and no observed structure changes, outperforming most reported PtCo catalysts and DOE 2025 targets. Furthermore, the carbon confinement proportion can be controlled by varying the thickness of the coated carbon shell, and this strategy is also applicable to the synthesis of double‐confined Pt1Fe1 and Pt1Cu1 intermetallic nanoparticles.
Stacking and Layer Parity Dependent Photoluminescence in ReS2
Authors: Poudyal Saroj , Barman Prahalad Kanti , Biswas Rabindra , Kravtsov Konstantin , Biswal Bubunu , Yadav Renu , Raghunathan Varun , Kruglov Ivan , Ghazaryan Davit A. , Misra Abhishek 
Published: February 2025

AbstractReS2 crystallizes in low crystal symmetry distorted triclinic (1T’) structure, and has anisotropic optical properties. Recently, two different stacking orders named as AA and AB phases, have been identified in ReS2 with distinct characteristics, such as stacking order dependent Raman shift, carrier dynamics, and second harmonic generation (SHG). However, the low crystal symmetry structure combined with different stacking sequences complicate the understanding of optical responses of this material and thus instigating the debate on interlayer coupling, number of excitonic emissions, and quantum confinement. In this work, the open questions on the optical responses of ReS2 are addressed by combining angle resolved photoluminescence (PL) at cryogenic temperatures and SHG spectroscopy on different numbers of layers of two polymorphs. Careful identification of stacking sequences allows to conclusively establish that few layer AA phase ReS2 has two excitons, while AB phase demonstrates five emission peaks. SHG and PL imaging confirm layer parity dependent optical responses for AB phase ReS2. Furthermore, bulk and few layer thick samples are found to have distinct PL, suggesting that bulk ReS2 cannot be assumed as decoupled monolayers. The findings of this study hold significance toward understanding the optical phenomena occurring in polymorphs of anisotropic 2D layered materials.
Multidimensional Engineering of Escherichia coli for Efficient Adipic Acid Synthesis From Cyclohexane
Authors: Wang Fei , Sun Huiqi , Deng Di , Wu Yuanqing , Zhao Jing , Li Qian , Li Aitao 
Published: February 2025

AbstractAdipic acid (AA), a key aliphatic dicarboxylic acid, is conventionally manufactured through energy‐intensive, multi‐step chemical processes with significant environmental impacts. In contrast, biological production methods offer more sustainable alternatives but are often limited by low productivity. To overcome these challenges, this study reports the engineering of a single Escherichia coli for efficient biosynthesis of AA starting from cyclohexanol (CHOL), KA oil (mixture of CHOL and cyclohexanone (CHONE)), or cyclohexane (CH). To start with, a comprehensive screening of rate‐limiting enzymes is conducted, particularly focusing on cytochrome P450 monooxygenase, followed by the optimization of protein expression using strategies such as protein fusion, promoter replacement, and genome editing. Consequently, an engineered E. coli capable of efficiently converting either KA oil or CH into AA is obtained, achieving remarkable product titers of 110 and 22.6 g L−1, respectively. This represents the highest productivity record for the biological production of AA to date. Finally, this developed biocatalytic system is successfully employed to convert different cycloalkanes and cycloalkanols with varied carbon chain lengths into their corresponding dicarboxylic acids, highlighting its great potential as well as broad applicability for industrial applications.
Deciphering the Oncogenic Landscape of Hepatocytes Through Integrated Single‐Nucleus and Bulk RNA‐Seq of Hepatocellular Carcinoma
Authors: Su Huanhou , Zhou Xuewen , Lin Guanchuan , Luo Chaochao , Meng Wei , Lv Cui , Chen Yuting , Wen Zebin , Li Xu , Wu Yongzhang , Xiao Changtai , Yang Jian , Lu Jiameng , Luo Xingguang , Chen Yan , Tam Paul KH , Li Chuanjiang , Sun Haitao , Pan Xinghua 
Published: February 2025

AbstractHepatocellular carcinoma (HCC) is a major cause of cancer‐related mortality, while the hepatocyte mechanisms driving oncogenesis remains poorly understood. In this study, single‐nucleus RNA sequencing of samples from 22 HCC patients revealed 10 distinct hepatocyte subtypes, including beneficial Hep0, predominantly malignant Hep2, and immunosuppressive Hep9. These subtypes were strongly associated with patient prognosis, confirmed in TCGA‐LIHC and Fudan HCC cohorts through hepatocyte composition deconvolution. A quantile‐based scoring method is developed to integrate data from 29 public HCC datasets, creating a Quantile Distribution Model (QDM) with excellent diagnostic accuracy (Area Under the Curve, AUC = 0.968‐0.982). QDM was employed to screen potential biomarkers, revealing that PDE7B functions as a key gene whose suppression promotes HCC progression. Guided by the genes specific to Hep0/2/9 subtypes, HCC is categorized into metabolic, inflammatory, and matrix classes, which are distinguishable in gene mutation frequencies, survival times, enriched pathways, and immune infiltration. Meanwhile, the sensitive drugs of the three HCC classes are identified, namely ouabain, teniposide, and TG‐101348. This study presents the largest single‐cell hepatocyte dataset to date, offering transformative insights into hepatocarcinogenesis and a comprehensive framework for advancing HCC diagnostics, prognostics, and personalized treatment strategies.
Cubic Phase‐Inducible Zwitterionic Phospholipids Improve the Functional Delivery of mRNA
Authors: Iwakawa Kazuki , Sato Rikako , Konaka Mariko , Yamada Yuma , Harashima Hideyoshi , Sato Yusuke 
Published: February 2025

AbstractLipid nanoparticles (LNPs) are clinically advanced delivery systems for RNA. The extensively developed structure of ionizable lipids greatly contributes to the functional delivery of mRNA. However, endosomal escape is one of the severe biological barriers that continue to render this process inefficient (e.g., less than 10%). Although LNPs contain phospholipids, their role is poorly understood, and there have been few attempts to perform the chemical engineering required to improve their functionality. Herein, a cubic phase‐inducible fusogenic zwitterionic phospholipid derived from 1,2‐dioleoyl‐3‐sn‐glycero‐phosphoethanolamine (DOPE), DOPE‐Cx is described, that is designed to correct this problem. The orientation of a zwitterionic head group of DOPE is engineered by attaching a series of hydrophobic moieties for zwitterionic intermolecular interaction with the head structure of phosphatidylcholine (PC), and this is followed by a lipid‐phase transition into non‐lamellar phases to facilitate membrane fusion‐mediated endosomal escape. A structure–activity relationship study reveals that DOPE‐Cx lipids with small hydrophobic chains induce cubic phases instead of a hexagonal phase when mixed with PC, which enhances the functional delivery of mRNA in the liver as opposed to the action of the typically utilized and naturally occurring phospholipids. Engineered functionalized phospholipids will be of great value for the therapeutic applications of mRNAs.
Linking Metastatic Behavior and Metabolic Heterogeneity of Circulating Tumor Cells at Single‐Cell Level Using an Integrative Microfluidic System
Authors: Hou Ying , Lin Jiaxu , Yao Hongren , Wu Zengnan , Lin Yongning , Lin Jin‐Ming 
Published: February 2025

AbstractCirculating tumor cells (CTCs) are pivotal biomarkers in tumor metastasis, however, the underlying molecular mechanism of CTCs behavioral heterogeneity during metastasis remains unexplored. Here, an integrative workflow is developed to link behavior characteristics to metabolic profiling within individual CTCs, which simulates the metastatic process on a microfluidic system and combined with single‐cell mass spectrometry (MS) detection. Spheroid‐derived HCT116 cells are tracked and extracted via a temporary vascular system, revealing various arrest patterns under biomimetic vascular shear flow. Downstream MS analysis characterizes 17 cellular metabolites and associates metabolic profiles with de‐adhesion behaviors of the same CTCs, identifying a potential high‐metastatic subpopulation with enhanced arrest ability and evaluating critical metabolites involved in metastasis pathways. Additionally, the metastasis‐inhibiting effect of anti‐tumor drug 5‐fluorouracil by reducing high‐metastatic cells in spheroids is elucidated. This approach offers a valuable opportunity to dissect the interplay of the metastatic behavior and metabolic profiles of CTCs and foster insights into the molecular mechanisms underlying behavioral phenotypes in the tumor metastasis process.