bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zinc oxide nanoparticles effect on thyroid and testosterone hormones in male rats
Authors: Luabi N M , Zayed N A , Ali LQ 
Published: October 2019

Abstract

Background: Due to their unique properties, zinc oxide nanoparticles (ZnO NPs) gained a broad utilization in nano-based industries and medicine, which may expose human to increased levels of NPs. Zinc oxide (ZnO) is one of the most commonly utilized material in diverse industrial fields such as dyes, additives, rubber, ceramics, chemical fibers, electronics, medical diagnosis, sunscreens, cosmetics, personal care products, and food additives. Nanomaterial describes tiny materials, decreasing the size of the nanoparticles leads to the increase in surface area and provides the absorption possibility for further chemical molecules on the surface and this increases the reactivity of the particles, leads to increase in the effects of toxicity in these materials, ZnO nanoparticles effectively absorb UV-A radiation, as they are used as biomarkers for Cancer Treatment and for Biomedical Applications, these nanoparticles display antifungal, and antibacterial effects.Exposure to zinc oxide nanoparticles has been increasing steadily, causing more attention being paid to their potential toxicity, including cytotoxicity and genotoxicity. Hence this study aimed to investigate the effect of ZnO NPs on thyroid hormone Triiodothyronine (T3), Thyroxine (T4) and (Thyroid stimulating hormone) TSH as well as testosterone hormone in male adult rats. While Methods: A total of 54Spargue-Dawley albino adult male rats were divided into nine groups each of 6 rats, daily treated intra-peritoneal with ZnO NPs two different doses (30, 60)mg/kg in three different periods of time (7,14 and 28) days, as following: Control groups (group 1,2,3): respectively received intra-peritoneal injection with distilled water for(7,14, and 28) days, Experimental groups (group 4,5,6): they were rats respectively received intra-peritoneal dose ( 60mg/kg ) of zinc oxide nanoparticles for (7,14, and 28) day, and (group 7,8,9) experimental groups: were rats respectively received intra-peritoneal dose (30 mg/kg) of zinc oxide nanoparticles for (7,14, and 28) days. theResult: Data showed high significant decrease(p<0.01) in level of T3 and T4 and level of testosterone also decrease at high and low dose for 7,14 and 28 days, while the level of TSH showed no significant change in all dose and duration of time. Results of the current study suggested the possible time and dose effects disrupting of AgNPs on thyroid gland function and testes in male rats
Serum Immunoglobulins (IgG and IgA) Level in Chronic Sinusitis and Otitis Media Patients
Authors: Waleed Mustafa Nisreen , Nabeel Elia Zaid 
Published: October 2019

Abstract
Sinusitis and otitis media are two of the most common indications for antimicrobial agents of upper respiratory tract infection. This study was aimed to determine the effect two chronic upper respiratory tract diseases (sinusitis and otitis media) on immunoglobulins levels via assessment the level of serum IgG and IgA in sinusitis and otitis media patients using single radial immune diffusion technique. A total of 100 sera samples were included in this study, all of them were adults, 40 samples collected from each of sinusitis and otitis media patients. Control group was included 20 samples from healthy donors. The mean of IgG concentrations in sinusitis patients (1078.6mg/dl) revealed significant decreasing comparing with control group (1249.82mg/dl), whereas IgG level in otitis media patients (1338.77mg/dl) recorded non significant alteration. IgA mean of otitis media (388.26 mg/dl) significantly decreased compared to control group (235.3 mg/dl) while IgA mean of sinusitis samples (244.4 mg/dl) did not record any alteration. This study concluded that chronic sinusitis cause decreased of IgG while chronic otitis media cause increased IgA level. In general the immunoglobulins concentrations were mild moderate.
16SrRNA sequencing as tool for identification of Salmonella spp isolated from human diarrhea cases
Authors: Kamil Jabbar AL Kaabi Hayder , Salman AL-Yassari Abdul K. 
Published: October 2019

Abstract
This study was conducted in different location of Babylon, (75) clinical specimens were collected, obtained from patients suffering from severe diarrhea were attended to Merjan teaching hospital and Private clinics in Babylon province, and suffer from severe diarrhea that was associated with fever as diagnosed by physician, The period of specimens collection extended from September 2017 to January 2018. Results showed that out of 75 studied specimens of patients, 14 specimens were Salmonella positive (18.66 %). Salmonella isolates were isolated and identified by using bacterial culturing on selective media and confirming tests such as 16S rRNA gene amplification, all results of these diagnosis methods referred to all isolates belong to Salmonella spp. Depending on sequencing method for nucleotide sequence for 660 bp of 16S rRNA gene we sent only 10 isolates to registration these sequences in Gene Bank-NCBI for diagnosis of these isolates on species level and obtaining accession numbers then phylogeny. Ten accession numbers were obtained from 16SrRNA and registration of 10 sequences of 16SrRNA gene at gene bank-NCBI include one accession number (MH109326.1) for Salmonella enterica subsp. enterica serovar Typhimurium, Five accession numbers (MH156032.1, MH109512.1, MH109501.1, MH155972.1 and MH156033.1 )belong to Salmonella enterica sub.sp enterica serovar Typhi. While Salmonella enterica sub.sp enterica serovar Paratyphi recorded under two accession number (MH155974.1 and MH156039) and Salmonella enterica sub.sp enterica serovar Enteritidis recorded under two accession number (MH109386.1 and MH155973.1) respectively.
Antimicrobial activity of silver nanoparticles fabricated from some vegetable plants
Authors: Abdul Elah Mohammad Dalia , Mohammad Taher Eman 
Published: October 2019

Abstract
In the recent years green synthesis of silver nanoparticles is being have become more advantage than over other biological process because they avoid elaborating process of culturing and maintaining the cells. Also, nanoparticles synthesized from plant extracts is favourite because it is less cost, environmental -friendly, rapid and facile method. Therefore, the present study we chosen some plant extracts represented with Petroselinum crispum, Beta vulgaris and Lepidium aucheri for fabrication Ag NPs which was treated with silver nitrate solution( 1mM ) at two temperatures (60-100)C°. Ag NPs synthesized was investigated visually by colour shift for reaction mixture from yellowish green to brown colour. synthesized Ag NPs was characterise by UV-Visible spectroscopy that revealed a characteristic surface Plasmon resonance peak between( 405- 422 )nm. Also using Fourier transform infra-red spectroscopy( FTIR) indicated the role of the functional groups that participated in synthetic process. These Ag NPs showed antimicrobial activity against some bacterial strains.
Wound Healing Capacity, Antibacterial Activity, and GC-MS Analysis of Bienertia sinuspersici Leaves Extract
Authors: Shwaish Tarik , Al-Imarah Faris JM , Jasim Fatimah A 
Published: October 2019

Abstract
This study evaluates the wound healing efficacy of Bienertia sinuspersici leaves, a recently discovered species in the Arabian Gulf. A topical cream was formalized from the leaves and its wound healing activity was evaluated for incision, excision, and burn wound models. Bienertia sinuspersici extract was prepared and used for preliminary phytochemical screening, Gas Chromatography-MS analysis, antibacterial assay (disk diffusion method S. aureus, S. pyogenes, E. coli, and P. aeruginosa), antioxidant study, and to formulate a cream that was used for wound healing clinical trial. The presence of glycosides, saponins, and tannins is not confirmed; GC-MS analysis indicates the presence of 3-O-Methyl-d-glucose (a sugar, 44.29% concentration) and l-(+)-Ascorbic acid 2,6-dihexadecanoate (fat-soluble Ascorbic acid, 9.19% concentration). Average zone of inhibition for gram positive and gram negative bacteria were respectively 12-18 and 14-21 mm. Epithelization period for incisions wounds was 6-8 days (negative control: 12 days) with high keratin production and decreased scarring. Healing in excision wounds was steadier, faster and infection-less, contrary to controls. Bienertia sinuspersici leaves promote rapid and steady wound healing for both excision and incision wounds, also its antibacterial activity seems to induce a rapid, infection-less healing process.
Microbiological evaluation of locally produced ice cream in Baquba city. Iraq
Authors: Jawdat Fadihl Suhail , Qassim Mohammad Sarmad , Muafaq Al-qrtani Yasser 
Published: October 2019

Abstract
This study aimed to evaluate the bacteriological quality of ice cream made locally and seller’s hands in different sites of Baquba city. The samples include 70 randomly collected ice cream and 25 sample taken from seller’s hands. All samples were free from salmonella and shigella also the absence of mold and yeast was concluded. The distribution of isolated bacteria from ice cream samples was as follow: %47, %34, %28, %26, %9 for Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, Streptococcus faecalis and Bacillus cereus while in seller’s hands were as %44, %24, %24, %24, %20, %8, %8 for the respectively Escherichia coli, Bacillus cereus, Staphylococcus aureus, Proteus spp., Streptococcus faecalis, Citrobacter spp. and Enertobacter aerogenes. Generally the pathogenic bacteria as Escherichia coli isolated in highest number in both ice cream and seller’s hands The range of bacterial total count for ice cream samples was between 3.3 × 105 and 5.2 × 105 cfu/g. The present study showed that the microbiological quality of the ice cream samples not fit the standard value. The results of this study encourage the necessity of providing hygienic conditions in the process, preparing, package and marketing to minimize the contamination of such a most consumed dairy product like ice cream.
Genetic Diversity among Pseudomonas aeruginosa Isolated From Different Sources in Al-Diwaniyah City, Iraq Using RAPD-PCR Technique
Authors: Raheem Lateef Al-Awsi Ghaidaa , Alsudani Ali A , Mahdy Jabir Saja , Al-Mudhaffar Mohammed 
Published: October 2019

Abstract

Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens. To characterizes P. aeruginosa strains that are widespread in patients in Al-Diwaniyah city, 30 clinical and environment samples were collected from general hospitals of Al-Diwaniyah city. Methods for isolation and identifying P. aeruginosa based upon culture methods coupled with biochemical tests, A total of 11 amplified DNA fragments ranging in size from 250 to 1 Kpb were observed using two random amplified polymorphic DNA (RAPD) primers (opX-13, op Y-04) where as22 polymorphic amplified fragments were commonly detected among the 6 p. aeuroginosa isolates and each of primer give different genetic profiles. The results shows the RAPD-PCR analysis is a good epidemiological screening method to detect genetic variation among pseudomonas aeuroginosa isolates.
Detection ssurRNA gene in children infected by giardiasis
Authors: Tektook NihadKhalawe , Pirko EptissamYounan , Amer Taha Tamara 
Published: October 2019

Abstract
Intestinal parasites that infect humans are widespread health problems, especially in tropical and semitropical zone, is no less important than other microscopic pathogens because it has the ability to infect most of the body’s organs and lead to sometimes fatal complications that end in death, in addition to its ability to reproduce and in great numbers, which led to the failure to eliminate it completely, parasitic protozoa and helminthes intestinal are a large variety of parasites that live in the intestine. This study aimed at investigating the infection of Giardia lambila in babies with diarrhea who have been referred to (the women’s hospital and children in Babylon, 200 samples of feces were collected form infants patients during the period from January 2018-July 2018, alaboratory microscopictestshowed topositive infection rate50/200 (25%), thise positive samples were examined secondly by Real-Time PCR as a more accurate detection and showed 41/50 (92.41%).
Effects of Cumin Seeds in some physiological characteristics ofquail
Authors: Mahmoud Alkattan Muntaha 
Published: October 2019

Abstract
The present trail was conducted to know the effects of Cumin seeds powder at different concentrates (250, 500, 1000, 2000) mg/kg B.W on some physiological and reproductive characters of male Quail bird’s. (Coturnix coturnix) The study was included (25) male of Quail birds their age and weight were (60-75) days, (161-180) g respectively, the experiment randomly divided equally into 5 groups,5 for each group First group was given a standard ration and empty capsules dose, considered the control group. Second group was given a standard ration and capsules contains Cumin seeds powder at 250 mg/kg B.W, Third group was given a standard ration and capsules contains Cumin seeds powder at 500 mg/kg B.W Fourth group was given a standard ration and capsules contains Cumin seeds powder at 1000 mg/kg B.W. Fifth group was given a standard ration and Cumin capsules at dose 2000 mg/kg B.W, this treatments was daily continue for 4 weeks. The results exhibited positive outcome significant through their effects on some physiological and reproductive characters where it rose by the concentration of the total antioxidants capacity (TAC) as compar with the control group, the increase was clearer by treating the birds with the 500 and 2000 mg/kg B.W concentrations, with (p ⩽ 0.05) possibility level. The hormonals concentrations were increased such as Testosterone, Spermatogenic Stimulating Hormone (SSH) and the Interstitial Cell Stimulating Hormone (ICSH) compared with the control group with (p ⩽ 0.05) whereas the Corticosterone decreased over the whole Cumin seeds powder treatment groups within all concentrations compared with the control group at (p ⩽ 0.05), the decrease was clearer within birds group treated with 2000 mg/kg B.W concentration. The study results suggest that the Cumin seeds powder has positive effects over concentration of the total antioxidants capacity for and some male sexual hormones while inhibition occurs in other hormones as cortisol.
Evaluation of DNA damage and Environmental Antioxidants defense systems in type 2 diabetes mellitus Patients
Authors: MmJ Almamoori Ayad , Al-Jassani Mohammed J , Khadairi Maher M , Alwan Wasan K , Nayyef Ali Assi Rasha 
Published: October 2019

Abstract
The presents study was conducted to evaluate the DNA damage markers, antioxidants makers and lipid peroxidation in type 2 diabetes mellitus patients, the results of presents study was showed the DNA damage markers such as comet length, tail length, %DNA in tail and tail moments were significantly increased at p<0.05 in diabetic patients as compared with healthy control group, Also all biochemical markers such as superoxide dismutase SOD, catalase CAT, Glutathione peroxidase GPx, Glutathione GSH and Malondialdehyde MDA were significantly elevated with diabetic patients as compared with healthy control
Gene expression of some cytokines in patients with Cutaneous leishmaniasis in Al-Diwanyah province
Authors: Hussein Dakeel Marwa , Jerri Yousif Jameel 
Published: October 2019

Abstract
The current study was conducted in the Department of Biology - Faculty of Education for Girls - University of Kufa for the period from March 2018 to August 2018, which aims to detect the genetic expression of the mRNA of some of the cytokines in the peripheral blood of patients with cutaneous leishmaniasis using RT-qPCR technique. The results showed that the relative quantification of gene expression of mRNA for IFN-γ, IL-4, IL-10 andTNF-α in the blood of patients with cutaneous leishmaniasis compared to control. The highest amount was TNF-α, followed byIFN-γ, IL-10 and IL-4, which was 15.9±4.9, 10.5±4.6, 7.6±3.7 and 3.7±1.1 respectively, compared with control of 2.3±1.2, 2.6±1.2, 2.6±1.2 and 1.9±1.3 respectively, with a statistically significant difference at P <0.05. The correlation results showed that all cytokines (IFN-γ, IL-4, IL-10, TNF-α) were significantly correlated with each other in terms of gene expression and the association was strong betweenIFN-γ and IL-10 (r = 0.88), followed by IFN-γand TNF-α (r = 0.75) and IL-10 and TNF-α, (r = 0.63) and then IL-4 and IL-10 (r = 0.54).The conclusion from this study that a cellular immune response is achieved by increasing the genetic expression of cytokines, IFN-γ, IL-4, IL-10 and TNF-α in the peripheral blood of patients with cutaneous leishmaniasis and all these cytokines are positively correlated with each other.
Study of some clinico-pathological parameters of Thalassemia patients in AL-Zahraa hospital
Authors: Falah Abd Alaali Zahraa , Mohammed Flyyih Najat , fadel Radhia hussain 
Published: October 2019

Abstract
Thalassemia: is a genetic disorder caused by a defect in production of one or more globin chains of hemoglobin (Hb).Thalassemia is classified into two major groups, namely, α-thalassemia and β-thalassemia according to the particular type of globin chain affected. Various complications caused by this disease including, progressive liver failure and abnormal kidney function.one hundred samples of thalassemia were analyzed at AL-Zahraa hospital education in AL-Najaf province. The patients are aged (10-30) years. The results are compared with control group (50) persons. This study included some of the clinic-pathological and hematological parameters such as some liver enzymes, ferritin, complete blood count, blood film, also age and blood group. The results were (50%) male and (50%) female patients while the control group are (25%) male and (25%) female. Most Patients were (64%) in the age of (10 -15 years), followed by (26%) in the age group of (16 -20 years) and only (5%) in age (21-25 years), also (5%) for age (26 -30 years). Most frequent blood group in this study was O+ is (30%) followed by B+ group (27%) and the other blood groups A+, AB+, A-, O-, AB-, B- were (25%), (11%), (3%), (2%), (1%), (1%) respectively. Levels of Serum Glutamic Oxaloacetic Transaminase, Serum Glutamic Pyruvic Transaminase and ferritin, in addition to, platelets and White Blood Cells count are higher than normal while Hemoglobin, Hematocrit and Red Blood Cells are decreased. Peripheral blood film of thalassemia patients showing microcytic and hypochromic anemia, in addition to, nucleated Red Blood Cell and large number of target cells. This study demonstrates that thalassemia patients have difference in liver function, serum content ferritin and hematological characteristics in comparison with control group.
Estimation of catalase activity and Malondialdehyde levels in blood groups ABO of PCOS patients
Authors: Jawad Hassan Rajwan , Mohammad Hasson Al-Husseini Alaa 
Published: October 2019

Abstract
The present study has been carried out at the Women’s and Educational Children’s Hospital in the city of Diwaniyah to determine level of oxidative stress indicators. (57) samples of patient women with PCOS were collected and observation of some clinical signs were followed, in addition to confirming the results of ultrasonic ultrasound tests, the group of women patient with PCOS was divided into four subgroups according to the type of blood group, (8) samples of blood group A, (21) sample Of the blood group B, (7) of the AB blood group and (21) of the blood group O. 30 samples of the non-polycystic ovarian syndrome women were selected. They did not suffer from chronic diseases and were considered a control group to measure the level the activity of Catalase enzyme (CAT) and concentration levels of malondialdehyde MDA as oxidative stress indicators by using the Spectrophotometer.The results showed a significant decrease (p<0.05) in the efficacy of catalase and a significant increase (p<0.05) in the level of the MDA in women with PCOS compared to control group, in addition to decrease of CAT enzyme activity and an increase in the level of MDA were more in the blood group O compared with the other blood groups.
Extraction Dyes From Two Natural Plants Olive Leaves and Beta vulgaris And The Uses In dyeing Textile.
Authors: Sadiq Mahdi AL-Khateeb Dalia 
Published: October 2019

AbstractThe method of dyeing the wool and cotton yarn was used in this research. The yarns were first set up and then the dye solution was present and the threads were then immersed in the dyeing bath. After completion of the dyeing process, three tests were conducted to measure the color of the dyes towards the external factors. These tests are:First. Test the identity of the dye towards of light.Second. Test the identity of the dye towards the sweating.Third. Test the identity of the dye towards washing.Fourth. Test the identity of the dye towards acidic and alkaline.The results showed that the natural and industrial cotton was the most reliable. The industrial and natural wool were the least proven. Thus, the plant dyes are not equally proven against all external factors, but we can say that they are permanent dyes. This term is called dyes that are permanent dyes towards the factors and conditions that are Find out when using it.
Relation of angiography to hematological, hormonal and some biochemical variables in coronary artery bypass graft patients
Authors: Qader Othman Goran , Salah Saeed Ranj , Hussein Kadir Dler , Jalal Taher Hataw 
Published: October 2019

Abstract
The present study was designed to investigate the relation between severity of atherosclerosis via angiography and alteration of some important biochemical, hormonal and hematological variables in patients underwent Coronary Artery Bypass Graft (CABG) surgery. Eighty adult patients underwent coronary angiography were included in this study, and a standardized case-control study of acute myocardial infarction was established through taking 20 healthy individuals. Diagnostic coronary angiography was performed by a team of expert cardiologists. The patients were grouped according the number of major epicardial coronary arteries into one vessel disease (1VD), two vessels disease (2VD) or three vessels disease (3VD). The evaluation of biochemical tests were performed. The results of association of measurements with the severity of disease showed the priority of cholesterol and its related indexes (especially LDL) rather than TG indicating the severity of atherosclerosis. While, blood glucose and HbA1c were not apparently related to the degree of atherosclerosis. Significant reduction of T3 hormone and platelets and elevation in MPV were recorded in patients suffering from three vessels occlusion. This finding suggested strong association between severity of atherosclerosis and LDL, MPV and T3 in CABG patients.