bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Potential Tumor-educated Platelets RNA Biomarkers for Pan Cancer Detection and Staging
Authors: Ge Xinxin , Yuan Liuxia , Hu Ying , Cheng Bin , Shoaib Khan Muhammad , Dai Kesheng 
Published: August 2020

Abstract
Cancer is a disturbing disease with high morbidity and mortality. Although medical technology has been developed rapidly, diagnosis of cancer is still complicated, difficult, as well as expensive. Furthermore, cancers are mostly diagnosed at an advanced stage. Platelets mRNA profiles are altered after educated by tumors. Thereby, tumor-educated platelets (TEPs) mRNA profiles have the potentially diagnostic value for cancers currently. We downloaded and analyzed the next-generation sequencing datasets, GSE68086 and GSE89843, by integrated bioinformatics. A total of 43 biomarker genes were selected for further pathway enrichment analysis and correlation analysis, as well as diagnostic analysis. Gene ontology (GO) analysis showed these 43 TEPs mRNAs were mostly involved in protein binding, extracellular matrix and cellular protein metabolic process. Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis revealed that genes were mainly enriched in metabolic process. Eventually, after taking these 43 genes into spearman correlation analysis and receiving operating characteristic (ROC) curve analysis, we identified: 1) TEPs RSL24D1 mRNA was negatively related to early pan cancer as compared to healthy controls, and potential for early pan cancer diagnosis with a sensitivity of 71.8%, and a specificity of 64.3%; 2) HPSE, IFI27, LGALS3BP, CRYM, HBD, COL6A3, LAMB2, and IFITM3 showed an upward trend in the expression from early to more advanced pan cancer stages. The sensitivity of the diagnostic value for pan cancer of these genes was 60.9%, 59.1%, 56.5%, 57.8%, 54.3%, 55.2%, 55.2%, 60.9%, and a specificity of 94.5%, 90.9%, 87.3%, 89.1%, 72.7%, 85.5%, 89.1%, 94.5% respectively; 3) ARL2, FCGR2A, and KLHDC8B were positively associated with advanced, metastatic pan cancer as compared to healthy controls and could be diagnostic indicators for advanced pan cancer with a sensitivity of 59.2%, 61.8%, 59.7%, and a specificity of 80%, 89.1%, 83.6%, respectively. In summary, our findings identified that the 12-gene TEP liquid-biopsy biomarkers will not only facilitate early diagnosis of pan cancer, but also be beneficial to pan cancer staging.
Clinical and Cytogenetical Characteristics-Related Nomograms for Assessing Outcome of Acute Myeloid Leukemia Patients Post IA Induction
Authors: Wang Na , Dai Kanchun , Desai Aakash , Ge Bei , Lin Weihong , Ye Haige 
Published: August 2020

Abstract
Background: Acute myeloid leukemia(AML) is a highly heterogeneous hematological malignancy. Despite, increase in treatment options for AML over the past decade, prognosis for AML remain dismal. Numerous prognostic models have been developed for this disease, however nomograms predicting long-term survival in AML patients after induction chemotherapy(IA regimen) have not been described.Method: We constructed nomograms to predict disease-free survival(DFS) and overall survival(OS) by analyzing the cohort of patients with de novo non-M3 AML patients who underwent induction chemotherapy between June 2008 to August 2019. We utilized univariable and multivariable Cox proportional hazards regression analyses to obtain the selected variables for the nomograms. The discriminative ability and calibration were tested using C statistics, calibration plots, and Kaplan-Meier curves.Results: A total of 360 patients who underwent induction chemotherapy with IA regimen were included in the study. Of these 55% were male with a median age was 48 years. Using the univariate and multivariate analyses, the following variables were identified in the prediction of DFS: age(HR, 1.770; 95%CI, 1.160-2.702; P = 0.008), Hb(HR, 0.634; 95%CI, 0.462-0.870; P = 0.005), albumin(HR, 0.473; 95%CI, 0.363-0.615; P < 0.001) and cyto/molecular risk group(intermediate vs. favorable: HR, 1.614; 95%CI, 1.128-2.309; P = 0.001; poor vs. favorable: HR, 2.459; 95%CI, 1.645-3.676; P < 0.001) at the time of diagnosis, and allo-HSCT treatment(HR, 0.341; 95%CI, 0.234-0.497; P < 0.001). Factors which predicted OS were Hb (HR, 0.616; 95%CI, 0.438-0.866; P = 0.005), albumin (HR, 0.448; 95%CI, 0.340-0.591; P < 0.001), cyto/molecular risk group (intermediate vs. favorable: HR, 1.558; 95%CI, 1.068-2.275; P = 0.021; poor vs. favorable: HR, 2.348; 95%CI, 1.523-3.620; P < 0.001), allo-HSCT treatment (HR, 0.256; 95%CI, 0.166-0.396; P < 0.001), and age (HR, 1.528; 95%CI 0.980-2.382; P = 0.061). The discriminative ability and calibration of the nomograms revealed good predictive ability as indicated by the C statistics (0.715 for DFS and 0.731 for OS). Conclusion: Independent predictors of survival and relapse risk after IA regimen for AML can be utilized to obtain survival nomograms. These nomograms were able to predict DFS and OS while having good calibration accuracy and discriminative ability on internal validation.
Clinical Application of Confidence Interval for Monitoring Changes in Tumor Markers to Determine the Responsiveness to Cancer Treatment
Authors: Cho Jooyoung , Seo Dong Min , Uh Young 
Published: August 2020

Abstract
Background: Tumor markers are used to monitor disease progression and determine the responsiveness to cancer treatment. However, there are no standardized criteria for monitoring serial tumor marker measurements. Herein, we have developed our own monitoring system for interpreting changes in tumor markers using overlapping 95% confidence intervals (CIs) to determine whether the changes are significant.Methods: Two-year data, including 117,289 results for 11 tumor markers in our laboratory, were analyzed. The distributions of absolutely delta% and cut-off values for certain percentiles were calculated. CI ranges for each tumor marker were set based on biological variation, and data were analyzed for each patient assessed at health check-ups and clinics, individually and overall.Results: Most tumor markers had low indices of individuality, with between inter-individual variability. The 95th percentile cut-offs for each tumor marker were much higher in the health check-up group than in the clinic group. In decreasing order, the percentages of results with no overlap in 95% CIs were thyroglobulin antigen, 14.9%; protein induced by vitamin K absence-II (PIVKA), 11.9%; prostate-specific antigen, 9.8%; and cancer antigen 72-4, 8.7%. After correction using the reference interval, the percentages decreased to less than 5%, except for PIVKA (10.9%).Conclusions: We suggest that our own monitoring system can serve as a criterion for delta check and auto-verification of tumor markers. Further studies are required to validate and demonstrate this concept in real clinical situations using actual clinical data reflecting disease progression in cancer patients and responsiveness to cancer treatment.
Infiltration of CD1a-Positive Dendritic Cells in Advanced Laryngeal Cancer Correlates with Unfavorable Outcomes Post-Laryngectomy
Authors: Minesaki Akimichi , Kai Keita , Kuratomi Yuichiro , Aishima Shinichi 
Published: August 2020

Abstract
Background: The prognosis of advanced laryngeal cancer is unfavorable despite the progress of multidisciplinary therapy. Dendritic cells (DCs) play a central role in antitumor immunity. Tumor-infiltrating CD1a+ DCs have been reported to be associated with clinical outcomes in carcinomas of various organs, but the clinical impact of CD1a+ DCs in laryngeal cancer has not been clear.Patients and Methods: We retrospectively analyzed the cases of 57 patients with Stage Ⅲ or Ⅳ laryngeal cancer who underwent a total laryngectomy. Immunohistochemistry for CD1a, S100, and CD8 was performed using representative resected specimens. CD1a+ DCs, S100+ DCs, and CD8+ cytotoxic T-lymphocytes (CTLs) were evaluated, and we divided the cases into high and low groups by the cut-off of the median value for each of these three parameters.Results: Compared to the CD1a-low group, the CD1a-high group had more advanced cases and showed significantly worse disease-specific survival (DSS) (p=0.0082) and overall survival (OS) (p=0.0324). The analyses of S100 DCs and CD8+ CTLs revealed no significant impact on clinical outcomes. Multivariate analyses indicated that the infiltration of CD1a+ DCs ​​​is an independent unfavorable prognostic factor in both DSS (p=0.0093) and OS (p=0.0132).Conclusions: Our results demonstrated that the infiltration of CD1a+ DCs was associated with unfavorable clinical outcomes. It is not yet known why the presence of CD1a+ DCs lead to an unfavorable clinical outcome in laryngeal cancer. Further studies are necessary to validate our results and clarify the function(s) of tumor-infiltrating CD1a+ DCs.
The Effect of Staged Surgical Treatment for Cubitus Valgus After Non-Union of Lateral Condylar Fracture of Distal Humerus in Older Children
Authors: Liu Xin , Xie Li-wei , Deng Zhi-qiang , Ye Jia-jun 
Published: August 2020

Abstract
Background: The purpose of this study was to investigate the effect of staged surgery (open reduction/internal fixation and osteotomy) for cubitus valgus after non-union of lateral condylar fractures of the distal humerus in older children.Methods: From January 2010 to January 2013, 9 patients were treated with two-staged surgery (open reduction/internal fixation and osteotomy). The study included 5 males and 4 females, with a mean age of 12.7 years. The minimum interval from fracture to the first surgery was 7 years and the maximum interval was 10 years (average 8.2 years). All patients had symptoms of injury of the ulnar nerve and instability of the elbow. The first surgery included internal reduction, internal fixation, and bone grafting, exposing the elbow through a Kocher lateral approach. The procedure included clearing the peripheric callus and proximal distal fracture end cicatrix with rongeur until cancellous bone was exposed, and fixation of the lateral condylar fragment with a hollow screw 4.0 mm in diameter and smooth Kirschner wire. The limb was immobilized in a long arm cast with the elbow at 90 degrees of flexion and the forearm in neutral rotation for 3 weeks, and active exercises were begun after removal. The internal fixation hardware was removed after 3 months. The second surgery, osteotomy of the supracondylar humerus, was completed after 6 months to correct cubitus valgus. Internal fixation from the osteotomy was removed 6 months later.Results: Six months after the second surgery, follow-up revealed that in all patients the lateral condylar fractures attained clinical union and cubitus valgus was corrected. Elbow function recovered well without arthrochalasis or humeral condylar avascular necrosis. All patients’ ulnar nerve injury symptoms disappeared.Conclusion: Staged surgery to treat cubitus valgus secondary to lateral condylar fracture non-union in older children serves to first provide elbow stability, then to correct cubitus valgus. Staged treatment may make up for the deficiencies of conventional treatment. However, due to our relatively short follow-up time, the long term effects are unknown.
Evaluation of Cardiovascular Risk Factors In Patients With Familial Hypercholesterolemia From The North-Eastern Area of Romania
Authors: Vlad Cristiana-Elena , Foia Liliana , Florea Laura , Costache Irina-Iuliana , Covic Andreea , Popescu Roxana , Reurean-Pintilei Delia , Covic Adrian 
Published: August 2020

Abstract
Background. Familial hypercholesterolemia (FH) is one of the most frequent and important monogenic cholesterol pathology. Traditional and nontraditional cardiovascular risk factors increase the prevalence of atherosclerotic cardiovascular disease (ASCVD) in this population. Objective. To establish the prevalence and the cardiovascular risk factors of FH population, to identify the ASCVD through the clinico-biological and imaging modifications during the 24-months follow-up.Methods. This first prospective study in the north-eastern part of Romania, carried out between October 2017-October 2019, out of 980 patients with dyslipidemia evaluated with the Dutch Lipid Network (DLCN) and Simone Broome (SM) scores, only 61 patients with DLCN score above 3 and FH possible/probably (SM score) were included.Results. The 61 FH subjects recorded a mean age of 48.46±12.53 years, with more women compared to men. Regarding the traditional cardiovascular risk factors, we identified that high blood pressure was the main factor in all patients, followed by sedentary lifestyle, obesity, smoker status, personal cardiovascular history and type 2 diabetes. The measured DLCN score recorded: “possible” FH identified in 39.4%, the “probable”FH in 45.9% and the “definite” FH in 14.7%. After the administration of the lipid-lowering agents for 24 months, TC and LDL-C levels, carotid intima-media thickness (cIMT) and ankle-brachial index (ABI) decreased and HDL-C levels increased, but without reaching the guideline goals. In addition, the high-dose of statin alone, the high-dose of statin with fenofibrate, subjects with „possible” FH, the normal values of cIMT and ABI, had a reduced time of ASCVD occurrence. Also, the high cIMT values, physical inactivity, high TC, TG and high-sensitivity C-reactive protein (hsCRP) levels were associated with an increased risk of ASCVD. Conclusions. To reduce cardiovascular risk, the FH patients need a cascade screening and a specific management. Even though it was the first observational study in the north-eastern part of Romania, further molecular genetics studies are needed to confirm the FH cases.
Detecting Hub Genes Associated With Lauren Subtype of Gastric Cancer by Weighted Gene Co-Expression Network Analysis
Authors: LIU XU , Yao Li , Qu Jingkun , Liu Lin , LIU XU , Wang Jiansheng , Calin George A. , Zhang Jia 
Published: August 2020

Abstract
Background Gastric cancer is a rather heterogeneous type of malignant tumor. Among the several classification system, Lauren classification can reflect biological and pathological differences of different gastric cancer.Method to provide systematic biological perspectives, we employ weighted gene co-expression network analysis to reveal transcriptomic characteristics of gastric cancer. GSE15459 and TCGA STAD dataset were downloaded. Co-expressional network was constructed and gene modules were identified. Result Two key modules blue and red were suggested to be associated with diffuse gastric cancer. Functional enrichment analysis of genes from the two modules was performed. Validating in TCGA STAD dataset, we propose 10 genes TNS1, PGM5, CPXM2, LIMS2, AOC3, CRYAB, ANGPTL1, BOC and TOP2A to be hub-genes for diffuse gastric cancer. Finally these ten genes were associated with gastric cancer survival. Conclusion More attention need to be paid and further experimental study is required to elucidate the role of these genes.
A risk score for predicting postoperative complications in non-intubated thoracic surgery
Authors: Lan Lan , Jiang Long , Duan Chongyang , Lu Weixiang , Zhang Canzhou , Cen Yanyi , He Jianxing 
Published: August 2020

Abstract
Background

The risk factors for postoperative complications in non-intubated video-assisted thoracoscopic surgery (VATS) has not been observed before. Here to develop a simple risk score to predict the risk of postoperative complications for patients who scheduling non-intubated VATS, which is beneficial to guide the clinical interventions.
Methods

1837 patients who underwent non-intubated VATS were included from January 2011 to December 2018. A development data set and a validation data set were allocated according to an approximate 3:2 ratio of total cases. The stepwise logistic regression was used to establish a risk score model, and the methods of bootstrap and split-sample were used for validation.
Results

Multivariable analysis revealed that the forced expiratory volume in the first second in percent of predicted, the anesthesia method, blood loss, surgical time, and preoperative neutrophil ratio were risk factors for postoperative complications. The risk score was established with these 5 factors, varied from 0–53, with the corresponding predicted probability of postoperative complications occurrence ranged from 1%-92%, and was calibrated (Hosmer-Lemeshow χ2 = 6.261; P = 0.618). Good discrimination was acquired in the development and validation data sets (C-statistic 0.705 and 0.700). A positive correlation was between the risk score and postoperative complications (P for trend < 0.01). Three levels of low-risk (0–15 points], moderate-risk (15–30 points], and high-risk (> 30 points] were established based on the score distribution of postoperative complications.
Conclusions

This simple risk score model based on risk factors of postoperative complications can validly identify the high-risk patients with postoperative complications in the non-intubated VATS, and allow for early interventions.
Temporary extracorporeal femoro-femoral crossover bypass to treat critical limb ischemia due to occlusive femoral transaortic microaxial left ventricular assist device – A novel technique and case series
Authors: Richarz Sabine , Siegemund Martin , d’Amico Rosalinda , Bachofen Bernadette , Döbele Thomas , Gürke Lorenz , Mujagic Edin 
Published: August 2020

Abstract
Background The Impella transaortic microaxial left ventricular assist device (MLVAD) is a temporary circulatory support (TCS). It's percutaneous insertion through the common femoral artery (CFA) bears the risk of severe ipsilateral limb ischemia. As long as the MLVAD is required for cardio - circulatory support, treatment options are limited. To approach this problem, we developed a temporary extracorporeal femoral - femoral crossover bypass to restore and maintain perfusion of the affected leg.The aim of this report is to introduce our novel technique and present results of a case series of accordingly treated patients.Methods From October 2018, we treated all patients with severe limb ischemia due to the MLAVD with a femoral - femoral crossover bypass and collected their data prospectively. For comparison, all consecutive patients undergoing placement of the MLAVD between January 2011 and July 2019 were identified retrospectively.The primary outcome of the study is the feasibility and safety of our percutaneously established extracorporeal femoral - femoral crossover bypass. As secondary endpoints, we report overall 30 - day mortality and limb salvage rates.Results Between January 2011 and July 2019, 25 of 245 (10.3%) patients developed a severe limb ischemia of the ipsilateral leg following the MLVAD placement.Until October 2018, 20 patients were treated conventionally (C - cohort) and since October 2018, five (consecutive) patients have been treated by an extracorporeal femoral - femoral cross over bypass (BP - Cohort).Following the BP - procedure, an immediate improvement of the perfusion of the affected limb was seen clinically and on Duplex ultrasound in all patients. 20% of the BP - cohort needed additional surgical salvage procedures compared to 25% of the C - Cohort. Limb salvage was documented in 100% of our patients and 30 days mortality was 60% in both groups.Conclusion This is the first case series reporting on a novel technique of limb salvage in patients with severe limb ischemia due to an MLVAD. We demonstrated that the percutaneous creation of an extracorporeal crossover bypass is feasible, safe and effective and should therefore be promoted.
Posterior Fixation Can Further Improve the Segmental Alignment of Lumbar Degenerative Spondylolisthesis with Oblique Lumbar Interbody Fusion
Authors: WU JINGYE , Ge Tenghui , Zhang Ning , Li Jianing , Tian Wei , Sun Yuqing 
Published: August 2020

Abstract
Background: For patients with degenerative spondylolisthesis, whether additional posterior fixation can further improve segmental alignment is unknown, compared with stand-alone cage insertion in oblique lumbar interbody fusion (OLIF) procedure. The aim of this study was to compare changes of the radiographical segmental alignment following stand-alone cage insertion and additional posterior fixation in the same procedure setting of OLIF for patients with degenerative spondylolisthesis.Methods: A retrospective observational study. Selected consecutive patients with degenerative spondylolisthesis underwent OLIF procedure from July 2017 to August 2019. Three radiographic parameters of disc height (DH), slip ratio and segmental lordosis (SL) were measured on preoperative CT scans and intraoperative fluoroscopic images. Comparisons of those radiographic parameters prior to cage insertion, following cage insertion and following posterior fixation were performed.Results: A total of thirty-three patients including six males and twenty-seven females, with an average age of 66.9±8.7 years, were reviewed. Totally thirty-six slipped levels were assessed with thirty levels at L4/5, four at L3/4 and two at L2/3. Intraoperatively, with only anterior cage support, DH was increased from 8.2±1.6mm to 11.8±1.7mm (p<0.001), the slip ratio was reduced from 11.1±4.6% to 8.3±4.4% (p=0.020) with the slip reduction ratio 25.6±32.3%, and SL was slightly changed from 8.7±3.7° to 8.3±3.0°(p=1.000). Following posterior fixation, the DH was unchanged (from 11.8±1.7mm to 11.8±2.3mm, p=1.000), the slip ratio was reduced from 8.3±4.4% to 2.1±3.6 % (p<0.001) with the slip reduction ratio 57.9±43.9%, and the SL was increased from 8.3±3.0° to 10.7±3.6° (p=0.008). Conclusions: Compared with stand-alone cage insertion, additional posterior fixation provides better segmental alignment improvement in terms of slip reduction and segmental lordosis in OLIF procedures in the treatment of lumbar degenerative spondylolisthesis.
Structural Characterization of Water-Soluble Polysaccharides from Sophora flavescensAit. and Their Anti-Inflammatory Evaluation Based on NO Release
Authors: Li Yi-Xuan , Linghu Ke-Gang , Jiang Si-Liang , Cui Yong-Sheng , Dong Cai-Xia , Yu Hua , Du Juan 
Published: August 2020

Abstract
Background: The dried roots of Sophora flavescens Ait. are traditionally used as Sophora Flavescens (Kushen in Chinese) to treat inflammatory diseases. It is traditionally served as a decoction, and polysaccharides represent one the major chemical constituents of this decoction. How about the structure of S. flavescens polysaccharides and whether they have anti-inflammatory activity should be uncovered.Methods: The purified polysaccharides were isolated through a combination of ion-exchange chromatography on DEAE 650 M and gel filtration on Superdex G-200 from hot water extract of S. flavescens. Structure was characterized by chemical derivatization as well as HPLC, FT-IR, GC-MS and NMR technologies. The preliminary in vitro antiinflammation activity was tested on RAW 264.7 cells upon NO release inhibition.Results: In this study, four polysaccharides, namely, SFNP-1, SFNP-2, SFAP-1, and SFAP-2, were isolated from S. flavescens. Results showed that both SFNP-1 and SFNP-2 contained (1→4)-linked glucans with small amounts of side chains at the O-4 position of the backbone chain residues. The two acidic polysaccharides (i.e.,SFAP-1 and SFAP-2) were identified to be pectin-type polysaccharides mainly containing a homo-galacturanan backbone consisting of α-(1→4)-linked GalAp and methyl-esterified α-(1→4)-linked GalAp residues at a ratio ofapproximately1:1. The bioactivity test revealed that the four purified polysaccharides have no cytotoxicity on RAW264.7andthat SFNP-1 and SFNP-2 show significant stimulating activity. Although the decoction of S. flavescens has been traditionally used as an anti-inflammatory agent, NO release inhibition results showed thatSFAP-1 and SFAP-2, as the major polysaccharides of SFCP, do not have significant anti-inflammatory effects. Conclusions: This result suggests that the anti-inflammatory effect of the decoction of S. flavescens may depend on the presence of alkaloids and not the polysaccharides it contains.