bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of bumetanide on neurodevelopmental impairments in patients with tuberous sclerosis complex: an open-label pilot study
Authors: van Andel Dorinde M. , Sprengers Jan J. , Oranje Bob , Scheepers Floortje E. , Jansen Floor E. , Bruining Hilgo 
Published: May 2020

Abstract
Background
Tuberous sclerosis complex (TSC) is an autosomal dominant disease that affects multiple organs including the brain. TSC is strongly associated with broad neurodevelopmental disorders, including autism spectrum disorder symptomatology. Preclinical TSC studies have indicated altered neuronal chloride homeostasis affecting the polarity of γ-aminobutyric acid (GABA) ergic transmission as a potential treatment target. Bumetanide, a selective NKCC1 chloride importer antagonist, may attenuate depolarizing GABA action, and in that way reduce disease burden. In this open-label pilot study, we tested the effect of bumetanide on a variety of neurophysiological, cognitive, and behavioral measures in children with TSC.

Methods
Participants were treated with bumetanide (2dd 0.5–1.0 mg) for 13 weeks in an open-label trial. The Aberrant Behavior Checklist-Irritability (ABC-I) subscale was chosen as the primary endpoint. Secondary endpoints included other behavioral questionnaires in addition to event-related potentials (ERP) and neuropsychological tests if tolerated. Additionally, the treatment effect on seizure frequency and quality of life was assessed. Endpoint data were collected at baseline, after 91 days of treatment and after a 28-day wash-out period.

Results
Fifteen patients (8–21-years old) with TSC were included of which 13 patients completed the study. Treatment was well-tolerated with only expected adverse events due to the diuretic effects of bumetanide. Irritable behavior (ABC-I) showed significant improvement after treatment in 11 out of 13 patients (t(12) = 4.41, p = .001, d = .773). A favorable effect was also found for social behavior (Social Responsiveness Scale) (t(11) = 4.01, p = .002, d = .549) and hyperactive behavior (ABC-hyperactivity subscale) (t(12) = 3.65, p = .003, d = .686). Moreover, patients rated their own health-related quality of life higher after treatment. At baseline, TSC patients showed several atypical ERPs versus typically developing peers of which prepulse inhibition was significantly decreased in the TSC group. Neuropsychological measurements showed no change and bumetanide had no effect on seizure frequency.

Limitations
The sample size and open-label design of this pilot study warrant caution when interpreting outcome measures.

Conclusions
Bumetanide treatment is a potential treatment to alleviate the behavioral burden and quality of life associated with TSC. More elaborate trials are needed to determine the application and effect size of bumetanide for the TSC population.
Trial registration
EU Clinical Trial Register, EudraCT 2016-002408-13 (www.clinicaltrialsregister.eu/ctr-search/trial/2016-002408-13/NL). Registered 25 July 2016.
Diagnostic value of PET/CT with 11C-methionine (MET) and 18F-fluorothymidine (FLT) in newly diagnosed glioma based on the 2016 WHO classification
Authors: Ogawa Tomoya , Kawai Nobuyuki , Miyake Keisuke , Shinomiya Aya , Yamamoto Yuka , Nishiyama Yoshihiro , Tamiya Takashi 
Published: May 2020

Abstract
Background
The molecular features of isocitrate dehydrogenase (IDH) mutation and chromosome 1p and 19q (1p/19q) codeletion status have pivotal role for differentiating gliomas and have been integrated in the World Health Organization (WHO) classification in 2016. Positron emission tomography (PET) with 3′-deoxy-3′-[18F]fluorothymidine (FLT) has been used to evaluate tumour grade and proliferative activity and compared with l-[methyl-11C]-methionine (MET) in glioma patients. Herein, we evaluated tracer uptakes of MET-PET/CT and FLT-PET/CT for differentiating glioma based on the 2016 WHO classification especially in relation to IDH1 mutation status.

Methods
In total, 81 patients with newly diagnosed supratentorial glioma were enrolled in this study. They underwent PET/CT studies with MET and FLT before surgery. The molecular features and histopathological diagnosis based on the 2016 WHO classification were determined using surgical specimens. The ratios of the maximum standardized uptake value (SUV) of the tumours to the mean SUV of the contralateral cortex (T/N ratios) were calculated on MET-PET/CT and FLT-PET/CT images.

Results
The mean T/N ratios of MET-PET/CT and FLT-PET/CT in IDH1-wildtype tumours were significantly higher than those in IDH1-mutant tumours (P < 0.001 and P < 0.001, respectively). Receiver operating characteristic analysis for differentiating IDH1 mutation status showed that the area under the curve of the FLT T/N ratio was significantly larger than that of the MET T/N ratio (P < 0.01). The mean T/N ratio of FLT-PET/CT in IDH1-wildtype tumours was significantly higher than that in IDH1-mutant tumours among grade II and III gliomas (P = 0.005), but this was not the case for MET-PET/CT. Both MET-PET/CT and FLT-PET/CT were able to distinguish between grade II and III gliomas in IDH1-mutant tumours (P = 0.002 and P < 0.001, respectively), but only FLT-PET/CT was able to distinguish between grade III and IV gliomas in IDH1-wildtype tumours (P = 0.029).

Conclusion
This study showed that FLT-PET/CT can be used to determine the IDH1 mutation status and evaluate glioma grade more accurately than MET-PET/CT. FLT-PET/CT can improve glioma differentiation based on the 2016 WHO classification, but caution must be paid for tumours without contrast enhancement and further studies should be conducted with more cases.
[99cmTc]Tc-PSMA-I&S-SPECT/CT: experience in prostate cancer imaging in an outpatient center
Authors: Werner P. , Neumann C. , Eiber M. , Wester H. J. , Schottelius M. 
Published: May 2020

Abstract
Background
Prostate-specific membrane antigen (PSMA) SPECT imaging in prostate cancer (PCa) could be a valuable alternative in regions where access to PSMA-PET imaging is restricted. [99mTc]Tc-PSMA-I&S is a new 99mTc-labeled PSMA-targeting SPECT agent, initially developed for radio-guided surgery. We report on the diagnostic use of [99mTc]Tc-PSMA-I&S-SPECT/CT in PCa.

Results
[99mTc]Tc-PSMA-I&S-SPECT/CT was performed and evaluated in 210 outpatients with PCa at a single center. Patients were imaged for biochemical recurrence (BCR, n = 152, mean PSA 8.7 ng/ml), for primary staging of high-risk PCa (n = 12, mean PSA 393 ng/ml), and restaging in advanced recurrent PCa (n = 46, mean PSA 101.3 ng/ml). Number and location of positive lesions were determined for the different subgroups. For BCR, detection rates were calculated, defined as the proportion of scans with at least one PSMA-positive lesion.
PSMA positive lesions were detected in 65.2% of all 210 patients. Tumor tissue was mainly detected in lymph nodes (59%), in the bone (42%), and in the prostate (fossa) (28%). In the subgroup of patients referred for detection of BCR the detection rate increased from 20% at a PSA level < 1 ng/ml to 82.9% and 100% at PSA levels > 4 ng/ml and > 10 ng/ml, respectively. In the subgroup of high-risk patients referred for primary staging, 42% demonstrated metastatic disease. Restaging of advanced recurrent PCa revealed detectability of PSMA positive tumor lesions in 85% of the scans.

Conclusions
[99mTc]Tc-PSMA-I&S-SPECT/CT was useful in PSMA-targeted imaging of PCa at various clinical stages. At low PSA levels (< 4 ng/ml), detection rates of [99mTc]Tc-PSMA-I&S-SPECT/CT in BCR are clearly inferior to data reported for PET-imaging and should thus only be considered for lesion detection if imaging with PET is unavailable. However, at higher PSA levels (> 4 ng/ml) [99mTc]Tc-PSMA-I&S-SPECT/CT provides high detection rates in BCR. [99mTc]Tc-PSMA-I&S-SPECT/CT can also be used for primary staging and for restaging of advanced recurrent PCa. However, further studies are needed to assess the clinical value in these indications.
Genetic diversity and population structure of Tenacibaculum maritimum, a serious bacterial pathogen of marine fish: from genome comparisons to high throughput MALDI-TOF typing
Authors: Bridel Sébastien , Bourgeon Frédéric , Marie Arnaud , Saulnier Denis , Pasek Sophie , Nicolas Pierre , Bernardet Jean-François , Duchaud Eric 
Published: May 2020

AbstractTenacibaculum maritimum is responsible for tenacibaculosis, a devastating marine fish disease. This filamentous bacterium displays a very broad host range and a worldwide geographical distribution. We analyzed and compared the genomes of 25 T. maritimum strains, including 22 newly draft-sequenced genomes from isolates selected based on available MLST data, geographical origin and host fish. The genome size (~3.356 Mb in average) of all strains is very similar. The core genome is composed of 2116 protein-coding genes accounting for ~75% of the genes in each genome. These conserved regions harbor a moderate level of nucleotide diversity (~0.0071 bp−1) whose analysis reveals an important contribution of recombination (r/m ≥ 7) in the evolutionary process of this cohesive species that appears subdivided into several subgroups. Association trends between these subgroups and specific geographical origin or ecological niche remains to be clarified. We also evaluated the potential of MALDI-TOF-MS to assess the variability between T. maritimum isolates. Using genome sequence data, several detected mass peaks were assigned to ribosomal proteins. Additionally, variations corresponding to single or multiple amino acid changes in several ribosomal proteins explaining the detected mass shifts were identified. By combining nine polymorphic biomarker ions, we identified combinations referred to as MALDI-Types (MTs). By investigating 131 bacterial isolates retrieved from a variety of isolation sources, we identified twenty MALDI-Types as well as four MALDI-Groups (MGs). We propose this MALDI-TOF-MS Multi Peak Shift Typing scheme as a cheap, fast and an accurate method for screening T. maritimum isolates for large-scale epidemiological surveys.
First record of experimentally induced salmon gill poxvirus disease (SGPVD) in Atlantic salmon (Salmo salar L.)
Authors: Thoen Even , Tartor Haitham , Amundsen Marit , Dale Ole Bendik , Sveinsson Karoline , Rønning Hans Petter , Grønneberg Estelle , Dahle Maria Krudtå , Gjessing Mona Cecilie 
Published: May 2020

AbstractSalmon gill poxvirus (SGPV) infection is a common denominator in many cases of complex gill disease in the Norwegian salmon farming industry and may, as a single agent infection, result in salmon poxvirus disease (SGPVD). Experiences from the field suggest that stress may be a decisive factor for the induction of SGPVD. Here we investigated the effect of stress hormone treatment on SGPV kinetics and disease development. In our experiment, Atlantic salmon were divided into four groups. Two groups of fish received an intraperitoneal injection of hydrocortisone dissolved in a fatty vehicle, whereas fish in the other two groups received a sham injection of the vehicle. After 24 h, one group with hydrocortisone injection and one with sham injection were exposed to dead SGPV-infected fish. Plasma cortisol level, virus kinetics, virus localization, and pathological gill were monitored for 4 weeks post-exposure. Hydrocortisone injected fish displayed higher plasma cortisol and SGPV loads than non-hydrocortisone treated fish. Signs of SGPVD and ensuing mortality appeared only in fish exposed to the virus and injected with hydrocortisone around 2 weeks post-exposure. No clinical signs of disease or mortality were recorded in the other groups. Further, gill histopathology in diseased fish correlated well with SGPV load, with the infection apparently confined to gill epithelial cells. The current findings suggest elevated plasma cortisol being a prerequisite for the development of SGPVD and recommend minimization of stressful farming activities, particularly if SGPV infection has been previously identified.
Preconditioned generalized orthogonal matching pursuit
Authors: Tong Zhishen , Wang Feng , Hu Chenyu , Wang Jian , Han Shensheng 
Published: May 2020

AbstractRecently, compressed sensing (CS) has aroused much attention for that sparse signals can be retrieved from a small set of linear samples. Algorithms for CS reconstruction can be roughly classified into two categories: (1) optimization-based algorithms and (2) greedy search ones. In this paper, we propose an algorithm called the preconditioned generalized orthogonal matching pursuit (Pre-gOMP) to promote the recovery performance. We provide a sufficient condition for exact recovery via the Pre-gOMP algorithm, which says that if the mutual coherence of the preconditioned sampling matrix Φ satisfies $ \mu ({\Phi }) < \frac {1}{SK -S + 1}, $μ(Φ)<1SK−S+1, then the Pre-gOMP algorithm exactly recovers any K-sparse signals from the compressed samples, where S (>1) is the number of indices selected in each iteration of Pre-gOMP. We also apply the Pre-gOMP algorithm to the application of ghost imaging. Our experimental results demonstrate that the Pre-gOMP can largely improve the imaging quality of ghost imaging, while boosting the imaging speed.
Hermite–Hadamard-type inequalities involving ψ-Riemann–Liouville fractional integrals via s-convex functions
Authors: Zhao Yong , Sang Haiwei , Xiong Weicheng , Cui Zhongwei 
Published: May 2020

AbstractIn this paper, we establish some new Hermite–Hadamard-type inequalities involving ψ-Riemann–Liouville fractional integrals via s-convex functions in the second sense. Meanwhile, we present many useful estimates on these types of new Hermite–Hadamard-type inequalities. Finally, we give some applications to special means of real numbers.
Analysis on control of a class of uncertain stochastic system by inequality technique
Authors: Lu Qinghua , Li Longsuo 
Published: May 2020

AbstractIn this paper, some theoretical results of PID control of second order nonlinear uncertain stochastic system are given via inequalities. We extend the results of the corresponding deterministic systems to stochastic systems. Specifically, as long as we have a certain understanding of the upper bound of the derivative of the unknown nonlinear drift term and diffusion term, an analytic design method can be constructed for these three PID parameters to ensure the global stability and asymptotic stability of the closed-loop control systems. In addition, the numerical simulation results verify the theoretical analysis results.
Cyclist detection and tracking based on multi-layer laser scanner
Authors: Zhang Mingfang , Fu Rui , Guo Yingshi , Wang Li , Wang Pangwei , Deng Hui 
Published: May 2020

Abstract
The technology of Artificial Intelligence (AI) brings tremendous possibilities for autonomous vehicle applications. One of the essential tasks of autonomous vehicle is environment perception using machine learning algorithms. Since the cyclists are the vulnerable road users, cyclist detection and tracking are important perception sub-tasks for autonomous vehicles to avoid vehicle-cyclist collision. In this paper, a robust method for cyclist detection and tracking is presented based on multi-layer laser scanner, i.e., IBEO LUX 4L, which obtains four-layer point cloud from local environment. First, the laser points are partitioned into individual clusters using Density-Based Spatial Clustering of Applications with Noise (DBSCAN) method based on subarea. Then, 37-dimensional feature set is optimized by Relief algorithm and Principal Component Analysis (PCA) to produce two new feature sets. Support Vector Machine (SVM) and Decision Tree (DT) classifiers are further combined with three feature sets, respectively. Moreover, Multiple Hypothesis Tracking (MHT) algorithm and Kalman filter based on Current Statistical (CS) model are applied to track moving cyclists and estimate the motion state. The performance of the proposed cyclist detection and tracking method is validated in real road environment.
Moving from information and collaboration to action: report from the 4th international dog health workshop, Windsor in May 2019
Authors: Pegram Camilla L. , Bonnett Brenda N. , Skarp Helena , Arnott Gareth , James Hannah , Hedhammar Åke , Leroy Gregoire , Llewellyn-Zaidi Aimée , Seath Ian J. , O’Neill Dan G. 
Published: May 2020

AbstractBackgroundDogs are the most popular mammal kept as a companion animal globally. Positive human-dog relationships can benefit both the human owners as well as the dogs. However, popularity as a companion animal species does not universally benefit dogs in reverse. Breed-related health problems in dogs have received increasing attention over the last decade, sparking increased concerns for dog welfare across many stakeholders. Progress towards improved welfare requires meaningful collaboration between all those working in dog health, science and welfare. The International Partnership for Dogs (IPFD), together with an alternating host organisation, holds biennial meetings called the International Dog Health Workshops (IDHW). The IPFD 4th IDHW was hosted by the UK Kennel Club in Windsor, UK in May 2019. With the aim of encouraging international and multi-stakeholder collaborations that are effective and ongoing, the 4th IDHW 2019 provided a forum to identify specific needs and actions that could improve health, well-being and welfare in dogs, building on outcomes and evaluating actions of previous IDHWs.ResultsThe workshop included 126 decision-leaders from 16 countries and was structured around five key themes identified as needing international, multi-stakeholder attention. These included the concept of “breed”, supply and demand, breed-specific strategies for health and breeding, genetic testing and extreme conformations. The review of progress made since the 3rd IDHW 2017 and the comprehensive lists of actions agreed upon during the current meeting suggest that movement from information and collaboration to action has been achieved. Working groups with specific tasks were identified and many plan to continue to communicate through forum communities onDogWellNet.com.ConclusionsThe IDHW provides a forum for formal and informal discussion between relevant groups so that key dog health and welfare issues can be identified and defined, and plans can be agreed for effective actions to address them. The 3rd IDHW 2017 resulted in a number of significant outcomes. New and continuing actions were laid down at the 4th IDHW 2019, which will be re-evaluated at the 5th IDHW facilitating continual progress.
HIV infection, and overweight and hypertension: a cross-sectional study of HIV-infected adults in Western Kenya
Authors: Saito Akiko , Karama Mohamed , Kamiya Yasuhiko 
Published: May 2020

Abstract
Background
Non-communicable diseases (NCDs) are increasing in Kenya, where HIV/AIDS remains a leading cause of death; however, few studies have investigated obesity and hypertension among adults with HIV infection. We conducted a cross-sectional study in Homa Bay, Western Kenya, during 2015 to determine the prevalence of overweight/obesity and hypertension among HIV-infected adults and to identify their risk factors.

Results
Anthropometric measurements and a structured questionnaire were administered to adults with HIV infection receiving care at Mbita Sub-county Hospital. A total of 251 HIV-positive individuals were enrolled. More women were overweight (17.2%) and obese (3.6%) than underweight (8.3%). The prevalence of abdominal obesity was high in women (62.7%), especially those aged 30–39 years. The prevalence of hypertension was 9.8% and 11.8% in men and women, respectively. Male participants tended to develop hypertension at an early age. Multivariate analysis showed that female sex was significantly associated with abdominal obesity. Regarding clinical factors, we identified an association between overweight and a history of opportunistic infections, as well as between hypertension and World Health Organization clinical stage. Sixty percent of HIV-infected participants assumed that a very thin body size indicated HIV infection.

Conclusions
The main findings of this study include a greater prevalence of overweight than underweight as well as a high prevalence of abdominal obesity among women. Social perception toward body size among people with HIV infection might remain problematic. Individuals living with HIV in Kenya should receive preventive intervention for overweight and abdominal obesity, with consideration of relevant social and cultural aspects.
Role of litter production and its decomposition, and factors affecting the processes in a tropical forest ecosystem: a review
Authors: Giweta Mekonnen 
Published: May 2020

AbstractIn the forest ecosystems, litterfall is an important component of the nutrient cycle that regulates the accumulation of soil organic matter (SOM), the input and output of the nutrients, nutrient replenishment, biodiversity conservation, and other ecosystem functions. Therefore, a profound understanding of the major processes (litterfall production and its decomposition rate) in the cycle is vital for sustainable forest management (SFM). Despite these facts, there is still a limited knowledge in tropical forest ecosystems, and further researches are highly needed. This shortfall of research-based knowledge, especially in tropical forest ecosystems, may be a contributing factor to the lack of understanding of the role of plant litter in the forest ecosystem function for sustainable forest management, particularly in the tropical forest landscapes. Therefore, in this paper, I review the role of plant litter in tropical forest ecosystems with the aims of assessing the importance of plant litter in forest ecosystems for the biogeochemical cycle. Then, the major factors that affect the plant litter production and decomposition were identified, which could direct and contribute to future research. The small set of studies reviewed in this paper demonstrated the potential of plant litter to improve the biogeochemical cycle and nutrients in the forest ecosystems. However, further researches are needed particularly on the effect of species, forest structures, seasons, and climate factors on the plant litter production and decomposition in various types of forest ecosystems.
Zebrafish as an alternative animal model in human and animal vaccination research
Authors: Bailone Ricardo Lacava , Fukushima Hirla Costa Silva , Ventura Fernandes Bianca Helena , De Aguiar Luís Kluwe , Corrêa Tatiana , Janke Helena , Grejo Setti Princia , Roça Roberto De Oliveira , Borra Ricardo Carneiro 
Published: May 2020

AbstractMuch of medical research relies on animal models to deepen knowledge of the causes of animal and human diseases, as well as to enable the development of innovative therapies. Despite rodents being the most widely used research model worldwide, in recent decades, the use of the zebrafish (Danio rerio) model has exponentially been adopted among the scientific community. This is because such a small tropical freshwater teleost fish has crucial genetic, anatomical and physiological homology with mammals. Therefore, zebrafish constitutes an excellent experimental model for behavioral, genetic and toxicological studies which unravels the mechanism of various human diseases. Furthermore, it serves well to test new therapeutic agents, such as the safety of new vaccines. The aim of this review was to provide a systematic literature review on the most recent studies carried out on the topic. It presents numerous advantages of this type of animal model in tests of efficacy and safety of both animal and human vaccines, thus highlighting gains in time and cost reduction of research and analyzes.