bluuebunny/crossref_metadata_2025_split huggingface dataset:
KARAKTERISTIK GEOKIMIA PHOSPHOR SEDIMEN PERMUKAAN PERAIRAN MANGUNHARJO (SEMARANG) DAN MARUNDA (JAKARTA)
Authors: Damardjati Noor Syafaat , Maslukah Lilik , Wulandari Sri Yulina 
Published: January 2020

ABSTRACTThe waters of Mangunharjo, Semarang and Marunda, Jakarta are located in the area of industrial and dense population. There is the activity of industrial, household, urban waste and agricultural in the land or along the river upstream can potentially as a source of pollutants and impacting the aquatic environment. Pollutants get into the water together with a suspension of sediment through river water flow and precipitation in the coastal environment. The study aims to determine the geochemical characteristics of sedimentary phosphor from two different locations. Determination of the phosphate fraction of sediment, using sequential determination extraction (SEDEX). The results showed that the concentration of the inorganic phosphorus sediment fraction has a similar relative distribution of Mangunharjo and Marunda waters i.e. Fe-P Ca-P Ads-P. The concentration of Ads-P, Ca-P, and Fe-P in Mangunharjo water is between 0.20-0.26 μmol g-1, 2.77-3.60 μmol g-1, and 4.60-5.74 μmol g-1 and Marunda water is between 0.28-0.61 μmol g-1, 2.91-4.13 μmol g-1, and 0.92-2.83 μmol g-1, respectively. The grain size of sediments and current patterns affect the distribution of phosphorus fraction.Keywords: Phosphorus fraction, surface sediments, Mangunharjo, MarundaABSTRAKPerairan Mangunharjo, Semarang dan Marunda, Jakarta berada didaerah kawasan industri dan padat penduduk. Terdapatnya aktivitas industri, rumah tangga, limbah perkotaan dan pertanian di daratan ataupun di sepanjang hulu sungai dapat berpotensi sebagai sumber pencemar dan berdampak bagi lingkungan perairan. Bahan pencemar masuk ke perairan bersama-sama muatan sedimen tersuspensi melalui aliran air sungai dan mengalami pengendapan di lingkungan pantai. Penelitian ini bertujuan untuk mengetahui karakteristik geokimia sedimen phosphor dari dua lokasi yang berbeda. Penentuan lokasi penelitian menggunakan metode purposive sampling. Penentuan fraksi phosphat sedimen, menggunakan ekstraksi bertingkat (SEDEX). Hasil penelitian menunjukkan bahwa konsentrasi dari fraksi inorganic phosphor sedimen memiliki distribusi relatif yang serupa antara Perairan Mangunharjo dan Marunda yaitu Fe-P Ca-P Ads-P. Konsentrasi Ads-P, Ca-P, dan Fe-P di Perairan Mangunharjo adalah antara 0.20 - 0.26 μmol g-1, 2.77 - 3.60 μmol g-1, dan 4.60 - 5.74 μmol g-1 dan Perairan Marunda adalah antara 0.28 - 0.61 μmol g-1, 2.91 - 4.13 μmol g-1, dan 0.92 - 2.83 μmol g-1, secara berurutan. Ukuran butir sedimen dan pola arus mempengaruhi konsentrasi fraksi fosfat pada kedua perairan tersebut.Kata Kunci : Fraksi phosphor, Sedimen permukaan, Mangunharjo, Marunda
Metabolic alterations provide insights into Stylosanthes roots responding to phosphorus deficiency
Authors: Luo Jiajia , Liu Yunxi , Zhang Huikai , Wang Jinpeng , Chen Zhijian , Luo Lijuan , Liu Guodao , Liu Pandao 
Published: January 2020

Abstract
Background: Phosphorus (P) deficiency is one of the major constraints limiting plant growth, especially in acid soils. Stylosanthes (stylo) is a pioneer tropical legume with excellent adaptability to low P stress, but its underlying mechanisms remain largely unknown.Results: In this study, the physiological, molecular and metabolic changes in stylo responding to phosphate (Pi) starvation were investigated. Under low P condition, the growth of stylo root was enhanced, which was attributed to the up-regulation of expansin genes participating in root growth. Metabolic profiling analysis showed that a total of 256 metabolites with differential accumulations were identified in stylo roots response to P deficiency, which mainly included flavonoids, sugars, nucleotides, amino acids, phenylpropanoids and phenylamides. P deficiency led to significant reduction in the accumulation of phosphorylated metabolites (e.g., P-containing sugars, nucleotides and cholines), suggesting that internal P utilization was enhanced in stylo roots subjected to low P stress. However, flavonoid metabolites, such as kaempferol, daidzein and their glycoside derivatives, were increased in P-deficient stylo roots. Furthermore, the qRT-PCR analysis showed that a set of genes involved in flavonoids synthesis were found to be up-regulated by Pi starvation in stylo roots. In addition, the abundances of phenolic acids and phenylamides were significantly increased in stylo roots during P deficiency. The increased accumulation of the metabolites in stylo roots, such as flavonoids, phenolic acids and phenylamides, might facilitate P solubilization and cooperate with beneficial microorganisms in rhizosphere, and thus contributing to P acquisition and utilization in stylo.Conclusions: These results suggest that stylo plants cope with P deficiency by modulating root morphology, scavenging internal Pi from phosphorylated metabolites and increasing accumulation of flavonoids, phenolic acids and phenylamides. This study provides valuable insights into the complex responses and adaptive mechanisms of stylo roots to P deficiency.
A feasibility study of a WhatsApp-delivered transtheoretical model-based intervention to promote healthy eating knowledge and habits for firefighters in Hong Kong: A cluster randomized controlled trial
Authors: NG Winnie WM , Cheung Kin 
Published: January 2020

Abstract
Background: Firefighters’ health is often affected by a high prevalence of obesity and cardiovascular diseases, which are common risk factors for sudden cardiac death. The aim of this study was to investigate the feasibility of enhancing healthy eating habits and knowledge in firefighters through an education programme. Method: This will be a cluster randomized control trial study. The participants will be assigned randomly into either control (health promotion pamphlet) or intervention (health promotion pamphlet and education materials through WhatsApp) groups. Changes in healthy eating habits and knowledge will be assessed by a self-administrative questionnaire (Appendix III) and anthropometric measurements at three different time points. Discussion: More education is required in order to improve firefighters’ eating habits and knowledge
Gene Co-expression Networks Are Associated with Obesity-Related Traits in Kidney Transplant Recipients
Authors: Jaime-Lara Rosario B , Roy Abhrarup Abe , Wang Yupeng , Stanfill Ansley , Cashion Ann K , Joseph Paule 
Published: January 2020

Abstract
Background Obesity is common among kidney transplant recipients; However biological mediators of obesity are not well understood in this population. Because subcutaneous adipose tissue can be easily obtained during kidney transplant surgery, it provides a unique avenue for studying the mechanisms of obesity for this group. Although differential gene expression patterns were previously profiled for kidney transplant patients, gene co-expression patterns can shed light on gene modules not yet explored on the coordinative behaviors of gene transcription in biological and disease processes from a systems perspective. Methods In this study, we collected 29 demographic and clinical variables and matching microarray expression data for 26 kidney transplant patients. We conducted Weighted Gene Co-expression Network Analysis (WGCNA) for 5,758 genes with the highest average expression levels and related gene co-expression to clinical traits. Results A total of 35 co-expression modules were detected, two of which showed associations with obesity-related traits, mainly at baseline. Gene Ontology (GO) enrichment was found for these two clinical trait-associated modules. One module consisting of 129 genes was enriched for a variety of processes, including cellular homeostasis and immune responses. The other module consisting of 36 genes was enriched for tissue development processes. Conclusions Our study generated gene co-expression modules associated with obesity-related traits in kidney transplant patients and provided new insights regarding the cellular biological processes underlying obesity in this population.
Tuberculosis Treatment Outcomes Among Prisoners and Non-Prisoners in Zomba, Malawi
Authors: Singano Victor Hamilton , Kip Esther , Ching’ani Wilson , Chiwaula Lawrence 
Published: January 2020

Abstract
Background TB remains a major global health problem. it is particularly prevalent in prisons in sub-Saharan Africa. Prisoners have experienced worse TB treatment outcomes than the general population. The researchers investigated the TB treatment outcomes and pre d ictors of unsuccessful treatment outcomes among prisoners and the general population. Methods We retrospectively reviewed TB registers of prisoners and the general population diagnosed with TB from January 2011 to December 2016 at Zomba Maximum Central Prison and Zomba Central Hospital, Malawi. The study used routinely collected data extracted from national, standardized TB treatment monitoring tools. Successful treatment outcome was classified as the total for cured and completed treatment while unsuccessful treatment outcome was classified as the total of deaths and treatment failures. We used descriptive statistics to compare the demographics and TB treatment parameters among prisoners and non – prisoners and computed multivariate analysis to predict the independent factors of unsuccessful treatment outcomes. Results Of 1652 registered cases, 27% were prisoners (all males) and 72% were non-prisoners (58 % males). The median age was 35 years (IQR: 29 - 42); 76 % were Pulmonary TB cases (78 % among prisoners vs 75% among non-prisoners); 83 % were new TB cases (77% among prisoners vs 86% among non-prisoners); and 65 % were HIV positive (50 % among prisoners vs 71% among non – prisoners). Regarding treatment outcome, 1472 (89%) were cured and/or completed treatment (93% among prisoners vs 88 % among non – prisoners), 2 (0.2%) were treatment failures, 122 (8%) died (5 % among prisoners vs 8 % among non – prisoners) and 55 (3%) were not evaluated (1% among prisoners vs 4% among non – prisoners). Unsuccessful TB treatment outcomes were associated with age greater than 35 years (aOR = 0.68: 95% C.I: 0.58 – 0.80), Extra-Pulmonary TB (aOR=1.69: 95% C.I: 1.08 – 2.63) andHIV positive status (aOR = 0.63: 95 % C.I: 0.42 – 0.94). Conclusion Maximum prisons provide a stable population that can be easily monitored during TB treatment. Good TB treatment outcomes comparable to the general population can be achieved among prisoners despite the challenging prison conditions.
Toric Intraocular Lens Implantation in Cataract Patients with Corneal Opacity.
Authors: Ra Ho , Hwang Ho Sik , Kim Hyun Seung , Kim Man Soo , Kim Eun Chul 
Published: January 2020

Abstract
Purpose: To evaluate the effect of toric intraocular lens implantation in cataract patient with corneal opacity and high astigmatism. Methods: 31 eyes of 31 patients who underwent cataract surgery with toric intraocular lens implantation were included. All patients had corneal opacity with astigmatism. Preoperative total corneal astigmatism was determined considering posterior astigmatism using a rotating Scheimpflug camera (Pentacam®: Oculus, Wetzlar, Germany). At 2 months after toric intraocular lens implantation, we evaluated residual astigmatism, uncorrected visual acuity (UCVA) and best corrected visual acuity (BCVA). Results: Postoperative UCVA and BCVA (0.30 ± 0.17, 0.22 ± 0.16LogMAR) were statistically improved compared to preoperative UCVA and BCVA (1.2 ± 0.34, 1.1 ± 0.30LogMAR, respectively) (P<0.01). Postoperative residual refractive astigmatism (1.2 ± 0.35D) was statistically reduced compared to preoperative refractive astigmatism (2.4 ± 0.65D) (P<0.05). Preoperative and postoperative total corneal astigmatism values were not statistically different. All eyes achieved postoperative visual acuity as good as or better than preoperative one. The size of corneal opacity covering pupil had significant negative correlation with postoperative UCVA and BCVA (logMAR) (R=0.91 P<0.05 and R=0.92 P<0.05, respectively) Conclusion: Toric intraocular lens implantation can improve UCVA, BCVA, and refractive astigmatism in cataract patient with corneal opacity. The size of corneal opacity covering pupil is the major prognostic factor for postoperative visual improvement. Therefore, toric intraocular lens implantation should be considered for cataract patients who have corneal opacity with high astigmatism.
Genetic mapping and genomic selection for maize stalk strength
Authors: Liu Xiaogang , Hu Xiaojiao , Li Kun , Liu Zhifang , Wu Yujin , Wang Hongwu , Huang Changling 
Published: January 2020

Abstract
Background Maize is one of the most important staple crops and is widely grown throughout the world. Stalk lodging can cause enormous yield losses in maize production. However, rind penetrometer resistance (RPR), which is recognized as a reliable measurement to evaluate stalk strength, has been shown to be efficient and useful for improving stalk lodging-resistance. Linkage mapping is an acknowledged approach for exploring the genetic architecture of target traits. In addition, genomic selection (GS) using whole genome markers enhances selection efficiency for genetically complex traits. In the present study, two recombinant inbred line (RIL) populations were utilized to dissect the genetic basis of RPR, which was evaluated in seven growth stages. Results The optimal stages to measure stalk strength are the silking phase and stages after silking. A total of 66 and 45 quantitative trait loci (QTL) were identified in each RIL population. Several potential candidate genes were predicted according to the maize gene annotation database and were closely associated with the biosynthesis of cell wall components. Moreover, analysis of gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway further indicated that genes related to cell wall formation were involved in the determination of RPR. In addition, a multivariate model of genomic selection efficiently improved the prediction accuracy relative to a univariate model and a model considering RPR-relevant loci as fixed effects. Conclusions The genetic architecture of RPR is highly genetically complex. Multiple minor effect QTL are jointly involved in controlling phenotypic variation in RPR. Several pleiotropic QTL identified in multiple stages may contain reliable genes and can be used to develop functional markers for improving the selection efficiency of stalk strength. The application of genomic selection to RPR may be a promising approach to accelerate breeding process for improving stalk strength and enhancing lodging-resistance.
Ten years risk assessment of Atherosclerotic cardiovascular disease using Astro-CHARM and Pooled Cohort Equation in a South Asian sub-population
Authors: Ashraf Tariq , Mengal Muhammad Naeem , Muhammad Atif Sher , Tareen Asal Khan , Khan Muhammad Nauman , Kazmi Khawar , Nadeem Asif , Sarwar Shakil , Bashir Zara , Qamar Nadeem , Karim Musa 
Published: January 2020

Abstract
Background Atherosclerotic cardiovascular diseases (ASCVD) are on the rise in low and middle-income countries attributed to modern sedentary lifestyle and dietary habits. This has led to the need of assessment of the burden of at-risk population so that prevention measures can be developed. The objective of this study was to assess ten years risk assessment of ASCVD using Astro-CHARM and Pooled Cohort Equation (PCE) in a South Asian sub-population.Methods A total of 386 residents of all six districts of Karachi with no ASCVD were enrolled in the study through an exponential non-discriminative referral snowball sampling technique. The inclusion criteria consisted of age 40 years or above and either gender. Study participants were enrolled after obtaining informed written consent and those study participants who were found to have either congenital heart disease or valvular heart diseases or ischemic heart disease were excluded from the study based on initial screening. For the calculation of 10 years risk of ACVD based on Astro-CHARM and PCE, the variables were obtained including medical history and coronary artery calcium and C-reactive protein measurements.Results Mean estimated 10-year risk of fatal or non-fatal myocardial infarction or stroke as per the Astro-CHARM was 13.98±8.01%, while mean estimated 10-year risk of fatal or non-fatal myocardial infarction or stroke as per the PCE was 22.26±14.01%. Based on Astro-CHARM, 11.14% of the study participants were labeled as having high risk, while PCE estimated 20.73% of study participants as having high risk of ASCVD.Conclusion Despite the fact that our findings showed substantial differences in ten-year risk of ASCVD between Astro-CHARM and PCE, both calculators can be used to develop a new population and specific risk estimators for this South Asian sub-population. Our study provides the first step towards developing a risk assessment guided decision-making protocol for primary prevention of ASCVD in this population.
Multimodal Cocktail Periarticular Injection vs Femoral Nerve Block After Total Knee Arthroplasty: A Simultaneous Bilateral Randomized Study
Authors: Zhang Ye , Zhang Yu , Sun Jian-Ning , Chen Wang , Chen Xiang-yang , Feng Shuo 
Published: January 2020

Abstract
Background Pain control after total knee arthroplasty (TKA) is important for early postoperative experience and knee rehabilitation. However, there is no consensus on the Pain management after TKA. Whether early analgesic effect and patient satisfaction can reach or even exceed the femoral nerve block has been controversial. The aim of our study was to compare the early analgesia effect with multimodal cocktail periarticular injection (MCPI) and Femoral nerve block (FNB) during simultaneous knee arthroplasty. Methods Our study was a prospective randomized, double-blind controlled trial. From December 2018 to August 2019, 85 patients (170 knees) who underwent bilateral total knee arthroplasty at our institution were randomly treated with MICP and FNB. Randomization was done via a computerized randomization list. The visual analogue scores for pain, bilateral knee range of motion (ROM), postoperative adverse events, and patients satisfaction were assessed preoperatively and two weeks postoperatively. This trial is registered at ClinicalTrials.gov, number ChiCTR1800020003. Results A total of 85 patients (170 knees) were enrolled for bilateral TKA. Finally, 80 (160 knees) patients were included for analysis and comparison. The operation time of the MCPI group was significantly lower than the FNB group (66.5±8.7 min compared with 72.8±9.5 min, P<0.05). postoperative 2nd，3rd and 5th days, the MCPI group in VAS for pain at rest and Straight elevated leg were significantly lower than FNB group (P<0.05). The knee flexion of MCPI group was statistically greater than FNB group on the postoperative 3rd, and 5th days (P<0.05). Conclusions MCPI analgesia is better than FNB after total knee arthroplasty, which can shorten the operation time, and evidently prolong the Postoperative effective analgesia duration. Higher patient satisfaction is beneficial to early postoperative recovery. Trial registration: Chinese Clinical Trials Registry, ChiCTR1800020003,11/12/2018
Genome-wide identification, phylogenetic and expressional analysis of the UXS gene family in cotton
Authors: Pan Yuxin , Wang Jinpeng , Wang Zhenyi , Liu Hengwei , Zhang Lan , Wang Li , Song Xiaoming , Ge Weina , Wang Xiyin 
Published: January 2020

Abstract
Background: UDP-glucuronate decarboxylase (UXS) is an enzyme in plants and participates in cell wall noncellulose. Previous research suggested that cotton GhUXS gene regulated the conversion of non-cellulosic polysaccharides and modulates their composition in plant cell walls, showing its possible cellular function determining the quality of cotton fibers. Here, we performed evolutionary, phylogenetic, and expressional analysis of UXS genes from cottons and other selected plants. Results: By exploring the sequenced cotton genomes, we identified 10, 10, 18, and 20 UXSs genes in Gossypium raimondii , Gossypium arboretum , Gossypium hirsutum and Gossypium barbadense , and retrieved their homologs from other representative plants, including 5 dicots, 1 monocot, 5 green alga, 1 moss, and 1 lycophyte. Phylogenetic analysis suggested that UXS genes could be divided into four subgroups and members within each subgroup shared similar exon-intron structures, motif and subcellular location. Notably, gene colinearity information indicates 100% constructed trees to have aberrant topology, and helps determine and use corrected phylogeny. In spite of conservative nature of UXS, during the evolution of Gossypium , UXS genes were subjected to significant positive selection on key evolutionary nodes. Expression profiles derived from RNA-seq data showed distinct expression patterns of GhUXS genes in various tissues and different development. Most of GhUXS gene expressed highly at 10, 20 and 25 DPA (day post anthesis) of fibers. Real-time quantitative PCR analysis GhUXS genes expressed highly at 20 DPA or 25 DPA. Conclusions: UXS is relatively conserved in plants and significant positive selection affects cotton UXS evolution. The comparative genome-wide identification and expression profiling would lay an important foundation to understanding the biological functions of UXS gene family in cotton species and other plants.
Diffusible and Volatile Antifungal Compounds Produced by Pseudomonas chlororaphis subsp. aurantiaca ST-TJ4 against Various Phytopathogenic Fungi
Authors: Kong Wei-Liang , Li Pu-Sheng , Wu Xiao-Qin , Wu Tian-Yu , Sun Xiao-Rui 
Published: January 2020

Abstract
Plant growth-promoting rhizobacteria can potentially be used as an alternative strategy to control plant disease. In this study, strain ST-TJ4 isolated from the rhizosphere soil of a healthy poplar was found to have strong antifungal activity against 11 phytopathogenic fungi in agriculture and forestry. Strain ST-TJ4 was identified as Pseudomonas chlororaphis subsp. aurantiaca based on 16S rDNA sequences. The bacterium can produce siderophores, cellulase, and protease, and has genes involved in the synthesis of phenazine, 1-phenazinecarboxylic acid, pyrrolnitrin, and hydrogen cyanide. Moreover, the volatile compounds released by strain ST-TJ4 can inhibit the mycelial growth of plant pathogenic fungi more than diffusible substances can. Based on volatile compound profiles of strain ST-TJ4 obtained from headspace collection and GC-MS/MS analysis, 1-undecene was identified. In summary, the results suggested that P. chlororaphis subsp. aurantiaca ST-TJ4 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.