bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of Three Osmotin-Like Proteins from Plumeria rubra and Prospection for Adiponectin Peptidomimetics
Authors: Freitas Cleverson D.T. de , Nishi Beatriz C. , do Nascimento Camila T.M. , Silva Maria Z.R. , Bezerra Eduardo H.S. , Rocha Bruno A.M. , Grangeiro Thalles B. , Oliveira João P.B. de , Souza Pedro F. Noronha , Ramos Márcio V. 
Published: January 2020

Background:
Osmotin-Like Proteins (OLPs) have been purified and characterized from
different plant tissues, including latex fluids. Besides its defensive role, tobacco osmotin seems to
induce adiponectin-like physiological effects, acting as an agonist. However, molecular information
about this agonistic effect on adiponectin receptors has been poorly exploited and other osmotins
have not been investigated yet.

Objective and Methods:
The present study involved the characterization of three OLPs from
Plumeria rubra latex and molecular docking studies to evaluate the interaction between them and
adiponectin receptors (AdipoR1 and AdipoR2).

Results:
P. rubra Osmotin-Like Proteins (PrOLPs) exhibited molecular masses from 21 to 25 kDa
and isoelectric points ranging from 4.4 to 7.7. The proteins have 16 cysteine residues, which are
involved in eight disulfide bonds, conserved in the same positions as other plant OLPs. The threedimensional
(3D) models exhibited the three typical domains of OLPs, and molecular docking
analysis showed that two PrOLP peptides interacted with two adiponectin receptors similarly to
tobacco osmotin peptide.

Conclusion:
As observed for tobacco osmotin, the latex osmotins of P. rubra exhibited compatible
interactions with adiponectin receptors. Therefore, these plant defense proteins (without known
counterparts in humans) are potential tools to study modulation of glucose metabolism in type II
diabetes, where adiponectin plays a pivotal role in homeostasis.
A Hidden Human Proteome Signature Characterizes the Epithelial Mesenchymal Transition Program
Authors: Vergara Daniele , Verri Tiziano , Damato Marina , Trerotola Marco , Simeone Pasquale , Franck Julien , Fournier Isabelle , Salzet Michel , Maffia Michele 
Published: January 2020

Background:
Molecular changes associated with the initiation of the epithelial to mesenchymal transition
(EMT) program involve alterations of large proteome-based networks. The role of protein products mapping
to non-coding genomic regions is still unexplored.

Objective:
The goal of this study was the identification of an alternative protein signature in breast cancer cellular
models with a distinct expression of EMT markers.

Methods:
We profiled MCF-7 and MDA-MB-231 cells using liquid-chromatography mass/spectrometry (LCMS/
MS) and interrogated the OpenProt database to identify novel predicted isoforms and novel predicted proteins
from alternative open reading frames (AltProts).

Results:
Our analysis revealed an AltProt and isoform protein signature capable of classifying the two breast
cancer cell lines. Among the most highly expressed alternative proteins, we observed proteins potentially associated
with inflammation, metabolism and EMT.

Conclusion:
Here, we present an AltProts signature associated with EMT. Further studies will be needed to define
their role in cancer progression.
Screening of an Alkaline CMCase-Producing Strain and the Optimization of its Fermentation Condition
Authors: Zhou Junmei , Yin Lianghong , Wu Chenbin , Wu Sijia , Lu Jidong , Fang Hailing , Qian Yongchang 
Published: January 2020

Objective:
Alkaline Carboxymethyl Cellulase (CMCase) is an attractive enzyme for the textile,
laundry, pulp, and paper industries; however, commercial preparations with sufficient activity at
alkaline conditions are scarce.

Methods:
High CMCase-producing bacterial isolate, SX9-4, was screened out from soil bacteria,
which was identified as Flavobacterium sp. on the basis of 16S rDNA sequencing.

Results:
The optimum pH and temperature for CMCase reaction were 8.0 and 55°C, respectively. Alkaline
CMCase was stable over wide pH (3.0-10.6) and temperature (25-55°C) ranges. Enzyme activity
was significantly inhibited by the bivalent cations Mn2+
and Cu2+, and was activated by Fe2+. To improve
the alkaline CMCase production of SX9-4, fermentation parameters were selected through onefactor-
at-a-time and further carried out by response surface methodologies based on a central composite
design.

Conclusion:
High CMCase production (57.18 U/mL) was achieved under the optimal conditions:
10.53 g/L carboxymethylcellulose sodium, 7.74 g/L glucose, 13.71 g/L peptone, and 5.27 g/L ammonium
oxalate.
Molecular Docking of 4-ethoxychalcones on Oxidoreductase/Pirin Inhibitors and Cytotoxic Evaluation on Breast/Skin Cancer Cell Lines
Authors: Harshitha Kishori Ramachandra , Sarojini Balladka Kunhanna , Narayana Badiadka , Lobo Anupam Glorious , Kalal Bhuvanesh Sukhlal 
Published: January 2020

Background:
The role of α, β unsaturated propenone derivatives, has attracted the
chemists for its biological importance. An attempt is made to reveal the interaction between breast
and skin cancer cell lines with the help of molecular docking studies.

Objective:
The study aimed to synthesize and characterize 4-ethoxychalcones for testing breast and
skin cancer targets.

Methods:
A series of chalcone analogues starting from 4-ethoxyacetophenone and substituted
aromatic aldehydes were synthesized, well-characterized and evaluated for their in vitro anticancer
activities against human breast cancer (MDA-MB-231) and human metastatic melanoma (A-375)
cell lines by MTT assay. Docking simulation was performed to study the drug-receptor interaction
of chalcone scaffold on the active site of target inhibitor bound to cytochrome P450 family
oxidoreductase for breast cancer and Pirin inhibiting target for skin cancer, respectively.

Results and Discussion:
After performing cytotoxic evaluation, it was observed that compounds
having a substitution at the para position showed better results compared to ortho and meta positions
for both the cell lines. Molecular docking studies revealed different types of interactions with
selected oxidoreductase and Pirin inhibiting targets. Ligand-protein interactions and morphological
changes are monitored by molecular dynamics.

Conclusion:
The presence of electron-withdrawing and donating groups on ring B marginally
affected IC50 and docking scores. The stability of the binding mode of ligands having high inhibitory
efficiency for compounds 8 and 10 predicted by docking studies was confirmed by molecular
dynamics simulation. The pharmacokinetic parameters were found to be within the acceptable
range. Further molecular dynamics study would provide the necessary information.
In silico Studies, Synthesis and Antitubercular Activity of Some Novel Quinoline - Azitidinone Derivatives
Authors: Chitre Trupti S. , Asgaonkar Kalyani D. , Vikhe Amrut B. , Patil Shital M , Garud Dinesh R. , Khedkar Vijay M. , Sarkar Dhiman , Nawale Laxman U. , Yeware Amar 
Published: January 2020

Background:
Diarylquinolines like Bedaquiline have shown promising antitubercular
activity by their action of Mycobacterial ATPase.

Objective:
The structural features necessary for a good antitubercular activity for a series of quinoline
derivatives were explored through computational chemistry tools like QSAR and combinatorial
library generation. In the current study, 3-Chloro-4-(2-mercaptoquinoline-3-yl)-1-
substitutedphenylazitidin-2-one derivatives have been designed and synthesized based on molecular
modeling studies as anti-tubercular agents.

Methods:
2D and 3D QSAR analyses were used to designed compounds having a quinoline scaffold.
The synthesized compounds were evaluated against active and dormant strains of Mycobacterium
tuberculosis (MTB) H37 Ra and Mycobacterium bovis BCG. The compounds were also tested
for cytotoxicity against MCF-7, A549 and Panc-1 cell lines using MTT assay. The binding affinity
of designed compounds was gauged by molecular docking studies.

Results:
Statistically significant QSAR models generated by the SA-MLR method for 2D QSAR
exhibited r2 = 0.852, q2 = 0.811, whereas 3D QSAR with SA-kNN showed q2 = 0.77. The synthesized
compounds exhibited MIC in the range of 1.38-14.59(μg/ml). These compounds showed
some crucial interaction with MTB ATPase.

Conclusion:
The present study has shown some promising results which can be further explored
for lead generation.
Insights into Nano-Photo-Thermal Therapy of Cancer: The Kinetics of Cell Death and Effect on Cell Cycle
Authors: Tabei Mousa , Zeinizade Elham , Beik Jaber , Kamrava S. Kamran , Nasiri Zahra , Ghaznavi Habib , Shakeri-Zadeh Ali 
Published: January 2020

Background:
Despite considerable advances in nano-photo-thermal therapy (NPTT), there have been
a few studies reporting in-depth kinetics of cell death triggered by such a new modality of cancer treatment.

Objective:
In this study, we aimed to (1) investigate the cell death pathways regulating the apoptotic responses
to NPTT; and (2) ascertain the effect of NPTT on cell cycle progression.

Methods:
Folate conjugated gold nanoparticle (F-AuNP) was firstly synthesized, characterized and then assessed
to determine its potentials in targeted NPTT. The experiments were conducted on KB nasopharyngeal
cancer cells overexpressing folate receptors (FRs), as the model, and L929 normal fibroblast cells with a low
level of FRs, as the control. Cytotoxicity was evaluated by MTT assay and the cell death mode (i.e., necrosis or
apoptosis) was determined through AnnexinV/FITC-propidium iodide staining. Next, the gene expression profiles
of some key apoptotic factors involved in the mitochondrial signaling pathway were investigated using
RT-qPCR. Finally, cell cycle phase distribution was investigated at different time points post NPTT using flow
cytometric analysis.

Results:
The obtained results showed that KB cell death following targeted NPTT was greater than that observed
for L929 cells. The majority of KB cell death following NPTT was related to apoptosis. RT-qPCR analysis
indicated that the elevated expression of Bax along with the depressed expression of Bcl-xL, Survivin and
XIAP may involve in the regulation of apoptosis in response to NPTT. Flow cytometric analysis manifested that
16-24 hours after NPTT, the major proportion of KB cells was in the most radiosensitive phases of the cell cycle
(G2/M).

Conclusion:
This study extended the understanding of the signaling pathway involved in the apoptotic response
to NPTT. Moreover, the potential effect of NPTT on sensitizing cancer cells to subsequent radiation therapy was
highlighted.
A Link Between Brain Insulin Resistance and Cognitive Dysfunctions: Targeting Ca2+/cAMP Signalling
Authors: Bergantin Leandro B. 
Published: January 2020

Background:
A correlation between cognitive dysfunctions and brain insulin resistance
has been established by several clinical and experimental studies. Consistent data support that
people diagnosed with brain insulin resistance, resulted from diabetes, have shown an increased
risk of presenting cognitive dysfunctions, clinical signs of dementia and depression, then speculating
a role of dysregulations related to insulin signalling in these diseases. Furthermore, it is currently
discussed that Ca2+ signalling, and its dysregulations, may be a factor which could correlate
with brain insulin resistance and cognitive dysfunctions.

Objective:
Following this, revealing this interplay between these diseases may provide novel insights
into the pathogenesis of such diseases.

Methods:
Publications covering topics such as Ca2+ signalling, diabetes, depression and dementia
(alone or combined) were collected by searching PubMed and EMBASE.

Results:
The controlling of both neurotransmitters/hormones release and neuronal death could be
achieved through modulating Ca2+ and cAMP signalling pathways (Ca2+/cAMP signalling).

Conclusion:
Taking into account our previous reports on Ca2+/cAMP signalling, and considering a
limited discussion in the literature on the role of Ca2+/cAMP signalling in the link between cognitive
dysfunctions and brain insulin resistance, this article has comprehensively discussed the role
of these signalling pathways in this link (between cognitive dysfunctions and brain insulin resistance).
Utilizing Iron Tailing, Sludge and Fly Ash to Prepare Ceramsites
Authors: Wang Zi , Chen Hongjun , Yu Chunhu , Xue Zeyang , Wang Pengxiang , Cong Qianmin , Pei Lizhai , Fan Chuangang 
Published: January 2020

Background:
The deposits of iron tailing will pose a great risk of environmental
pollution and serious landscape impact which will affect the quality of life of humans.
Therefore, it is urgent to utilize iron tailing to produce valuable products.

Methods:
The tailing ceramsites were analysed by X-ray diffraction (XRD) and Scanning
Electron Microscopy (SEM). The roles of the tailing content, sintering temperature and duration
time in the performance of the tailing ceramsites were analysed and the optimal sintering
parameters were determined.

Results:
The bulk density, apparent density and cylinder compressive strength of the tailing
ceramsites increase considerably with the increase of the sintering temperature and duration
time. The cylinder compressive strength of the tailing ceramsites increases with increasing
the tailing content. The optimal sintering parameter is 1100°C for 40 min. The cylinder compressive
strength of the tailing ceramsites obtained at 1100°C for 40 min reaches 10.1 MPa.
XRD analysis shows that the tailing ceramsites mainly consist of CaSiO3, Al2SiO5, MgSiO3,
Ca7Si2P2O16, CaAl2Si2O8, Ca2Fe2O5 and SiO2 phases when the sintering temperature and duration
time were increased to 1100°C and 40 min, respectively.

Conclusion:
The tailing ceramsites were obtained from iron tailing, sludge and fly ash as the
raw materials at 1100°C for 40 min. The obtained ceramsites exhibited high mechanical performance.
Produção científica em foco
Authors: Daré Paula Serafim , Vallada Candido 
Published: January 2020

A Self, revista do Instituto Junguiano de São Paulo (IJUSP), chega em 2020 ao seu quinto ano de atividade, mantendo-se firme em seu objetivo maior: o de promover e divulgar o pensamento junguiano com critérios científicos e padrões de publicação internacionalmente reconhecidos.
Com um conteúdo de alto nível, a Self pode ser acessada gratuitamente on-line por qualquer pessoa, democratizando assim o conhecimento de qualidade. Todo o processo editorial é realizado dentro da plataforma digital da revista, que adota o sistema rolling pass de publicação: os artigos são publicados na medida em que são aprovados, o que traz agilidade à divulgação.
Analisa Tegangan Pada Pipa Baja Karbon API 5L-GradeB Terhadap Laju Korosi Dalam Larutan NaCl dan Asam Asetat (Halaman 9 s.d. 12)
Authors: Suhendi Syafei Nendi , Suyaningsih Sri , Nurhilal Otong , Luthfiani Febi 
Published: January 2020

Efisiensi pemakaian energi untuk suatu kebutuhan harus dilakukan agar pemborosan energi dapat dihindarkan dengan melakukan pemilihan dan pemeliharaan peralatan logam. Korosi menjadi permasalahan utama pada industri minyak bumi dan gas karena dapat menghambat proses produksi. Korosi retak tegang (SCC, Stress Corrosion Cracking) adalah peristiwa pembentukan dan perambatan retak dalam logam yang terjadi secara simultan antara tegangan yang bekerja pada bahan dengan lingkungan yang korosif. Dalam penelitian ini digunakan rumah sampel dengan spesimen tiga titik pembebanan untuk melihat perilaku korosi retak tegang pada pipa baja karbon di lingkungan gas H2S kondisi jenuh CO2 dalam larutan NaCl dan Asam Asetat . Dalam larutan NaCl tidak terjadi korosi retak tegang (crack) pada baja karbon dengan laju korosi minimum 0,071 mmpy dan laju korosi maksimum 0,092 mmpy. Dalam larutan asam asetat terjadi korosi retak tegang (crack) pada baja karbon dengan laju korosi 0,25 mmpy dan 0,29 mmpy.
Kajian Pembuatan Oksida Grafit untuk Produksi Oksida Grafena dalam Jumlah Besar (Halaman 26 s.d. 29)
Authors: Syakir Norman , Nurlina Rhesti , Anam Syafiul , Aprilia Annisa , Hidayat Sahrul , - Fitrilawati 
Published: January 2020

Oksida grafit (GO) telah berhasil disintesis menggunakan metode Hummers dari serbuk grafit dengan pelarut asam sulfat (H2SO4), natrium nitrat (NaNO3), kalium permanganat (KMnO4), dan hidrogen peroksida (H2O2). Hasil sintesis berupa pasta dicuci dengan asam klorida (HCl) dan akuades berulang-ulang untuk mendapatkan GO yang relatif murni. Pasta tersebut selanjutnya dikeringkan dan digerus untuk karakterisasi EDS (Energy Dispersive Spectroscopy), difraksi XRD (X-ray Difraction), dan spektroskopi FTIR (Fourier Transform Infrared). Untuk mengupas lembaran GO menjadi lapisan lebih tipis, pasta yang dihasilkan tersebut didispersikan dalam akuades, kemudian disonikasi serta disentrifugasi. Hasil yang didapat dikarakterisasi dengan menggunakan spektroskopi UV-Vis. Hasil karakterisasi sampel serbuk menunjukkan telah terjadi perubahan struktur grafit dengan terbentuknya gugus-gugus fungsional oksigen.
Kajian Sifat Dielektrik pada Lempeng Nanopartikel Magnetit (Fe3O4) yang dienkapsulasi dengan Polyvinyl Alcohol (PVA) (Halaman 34 s.d. 37)
Authors: - Mustawarman , - Heriansyah , Suharyadi Edi 
Published: January 2020

Telah dilakukan sintesis nanopartikel Fe3O4 yang kemudian dienkapsulasi dengan Polyvinyl alcohol (PVA) dengan perbandingan 1:0, 1:3, 1:4, 1:5 dan 1:6 menggunakan metode kopresipitasi. Pengukuran dilakukan menggunakan sistem spektroskopi impedansi terkomputerisasi pada wilayah frekuensi 10 kHz hingga 120 kHz untuk mengetahui sifat dielektrik dari nanopartikel. Hasil pengukuran sifat dielektrik menunjukkan nilai permitivitas dielektrik pada seluruh sampel mengalami penurunan dengan bertambahnya frekuensi. Penambahan PVA mempengaruhi sifat dielektrik dari nanopartikel Fe3O4. Nilai permitivitas dielektrik riil seluruh sampel secara berturut-turut pada frekuensi 10 kHz yaitu 522, 212, 36, 234 dan 692. Nilai permitivitas dielektrik yang paling baik adalah nanopartikel Fe3O4+PVA (1:6 ) sehingga dapat digunakan untuk aplikasi biosensor Surface Plasmon Resonance(SPR) sebagai bahan aktif untuk meningkatkan sensitifitas sensor dan mengikat biomolekuler.
Karakterisasi Mikrostruktur Material Feroelektrik Ba0.8Sr0.2TiO3 (BST) dengan Variasi Suhu Annealing (Halaman 70 s.d. 72)
Authors: Dewi Rahmi , - Krisman , - Khaironiati , - Fauziana 
Published: January 2020

Sampel Barium Strontium Titanate, Ba0.8Sr0.2TiO3 (BST) telah disediakan dengan menggunakan reaksi kimia padatan. Mikrostruktur sampel telah dikarakterisasi dengan menggunakan teknik difraksi sinar-X (XRD) dan Scanning Electron Microscopy (SEM). Sampel disediakan dengan variasi suhu yang berbeda yaitu pada suhu 600oC, 700 oC dan 800oC selama 60 menit di udara. Karakterisasi XRD menunjukkan dengan meningkatnya suhu annealing maka kristaline sampel semakin meningkat dengan struktur tetragonal. Karakterisasi SEM menunjukkan sampel adalah homogen dengan ukuran butiran seragam. Ukuran butiran sampel akan bertambah dengan bertambahnya suhu annealing. Seluruh sampel mempunyai ukuran butiran dalam nanometer.
Karakterisasi Kekristalan dan Konstanta Dielektrik Ba0,9Sr0,1TiO3 yang Dibuat dengan Metode Solid State Reaction (Halaman 13 s.d. 15)
Authors: Maharsi R. , Jamaludin A. , Iriani Y. 
Published: January 2020

Ba0,9Sr0,1TiO3 (BST) telah dibuat dengan metode solid state reaction. Sampel disintering pada suhu 800 °C dan 900 °C selama 4 jam. Uji kekristalan dilakukan dengan instrumen X-Ray Diffractometer (XRD). Nilai konstanta dielektrik diperoleh melalui pengujian dengan RLC meter. Berdasarkan analisa dengan software GSAS, parameter kisi BST dengan suhu sintering 800 °C adalah a=b=3,981 nm dan c=3,999 nm. Parameter kisi a =b=3,986 nm dan c=3,988 nm untuk sampel BST dengan suhu sintering 900 °C. Sudut α=β =γ=90° untuk kedua sampel sehingga struktur kristalnya adalah tetragonal. Ukuran kristal sampel dengan suhu sintering 800 °C dan 900 °C adalah 45,31 nm dan 61,62 nm. Pengukuran konstanta dielektrik (K) dilakukan pada rentang frekuensi 0,01 hingga 10 kHz dimana nilainya maksimum pada nilai frekuensi minimum. Nilai K sampel dengan suhu sintering 800 °C dan 900 °C masing-masing sebesar 1920 dan 3650 pada frekuensi minimum.
Karakterisasi Mikrostruktur Material Feroelektrik Ba0.3Sr0,7TiO3 (BST) dengan variasi suhu annealing
Authors: Dewi Rahmi , Krisman Krisman 
Published: January 2020

Telah dilakukan pembuatan sampel dari bahan Barium Strontium Titanat (BST) dengan komposisi Ba0,3Sr0,7Tio3 dengan menggunakan metode sol gel dan di annealing pada suhu 600oC,650oC dan 700oC. Ukuran butira dan ketebalan sampel dikarakterisasi menggunakan Field Emission Scanning Microscopy (FESEM). Ukuran butiran meningkat dengan meningkatnya suhu annealing. Nilai rata-rata ukuran butiran dari temperatur 600oC, 650oC dan 700oC adalah : 21,20 nm; 22,30 nm dan 26.8 nm. Nilai ketebalan sampel masing-masing adalah 31,28 nm;37,5nm dan 39,08nm.
Modus Operandi dan Problematika Penanggulangan Tindak Pidana Penipuan Daring
Authors: Hendrik S. Anton 
Published: January 2020

AbstractOnline fraud experienced by the society in general perspective, and the East Java community in particular. The victimVictims hope is hoping that the perpetrator can be arrested, and the victim's loss got returned. Beside the technical constraints faced by law enforcement regarding privacy and data security regimes that also protect perpetrators, there is also problem in the rule of law. The norm specifically regulated in Article 28 paragraph (1) Law 11 of 2008 on Electronic Transaction Information (UU ITE) has a special restriction because of its consumer protection dimension. This article is a result of a research conducted regarding the profile of the modus operandi of online fraud in East Java and outlining the emerging legal problems. The research method used is juridical empiric and juridical normative, empiric juridical used to gather data, whereas juridical normative by literature study. The research findings are: it is necessity to reformulate the norms related to online fraud in the ITE Law because in its modus, there are cases of online fraud which not covered by Article 28 paragraph (1) of the ITE Law. IntisariTindak pidana penipuan daring cukup banyak dialami masyarakat pada umumnya, dan masyarakat Jawa Timur pada khususnya. Korban sangat berharap pelakunya dapat ditangkap kemudian uang atau kerugian korban dikembalikan. Selain kendala teknis yang dialami penegak hukum dalam rezim privasi dan keamanan data yang juga melindungi pelaku, juga terdapat problematika dalam pengaturan norma hukum. Norma yang diatur secara khusus dalam Pasal 28 ayat (1) UU No. 11 Tahun 2008 tentang Informasi Transaksi Elektronik (UU ITE) memiliki batasan khusus karena dimensi perlindungan konsumennya. Tulisan berikut ini merupakan hasil penelitian tentang profil modus operandi tindak pidana penipuan daring di Jawa Timur, penormaannya terkait tindak pidana tersebut dan menguraikan problematika hukum yang muncul. Metode penelitan yang digunakan adalah yuridis empiris dan yuridis normatif, yuridis empiris dilakukan dengan pengambilan dan pengolahan data sedangkan yuridis normatif dengan cara studi kepustakaan. Temuan penelitian adalah perlunya reformulasi norma terkait penipuan daring dalam UU ITE karena dalam profil modusnya terdapat kasus penipuan daring yang tidak tercakup dalam Pasal 28 ayat (1) UU ITE.