bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft genome sequences of two Streptomyces albidoflavus strains DEF1AK and DEF147AK with plant growth-promoting and biocontrol potential
Authors: Kunova Andrea , Cortesi Paolo , Saracchi Marco , Migdal Guy , Pasquali Matias 
Published: January 2021

Abstract
Purpose
Bacteria belonging to the Streptomyces genus can be exploited in environmentally friendly approaches to food safety. Genome information can help to characterize bioactive strains opening the possibility to decipher their mechanisms of action.

Methods
The biocontrol and plant growth-promoting activity of two Streptomyces spp. strains, DEF1AK and DEF147AK, were assessed in vitro and in planta. The genome sequences were determined using the Illumina NextSeq sequencing system and were assembled using EvoCAT (Evogene Clustering and Assembly Toolbox).

Result
Streptomyces spp. DEF1AK and DEF147AK were able to improve seed germination and early plant development of maize, wheat, and tomato and inhibited the mycelium growth of diverse fungal plant pathogens in vitro. The genome sequence analysis identified both strains as S. albidoflavus (99% sequence identity). Both genomes were of 7.1-Mb length with an average GC content of 73.45%. AntiSMASH and MIBiG analyses revealed strain-specific sets of secondary metabolite gene clusters in the two strains as well as differences in the number and type of duplicated genes.

Conclusion
The combination of the biological activity and genomic data is the basis for in-depth studies aimed at the identification of secondary metabolites involved in plant growth-promoting and biocontrol activity of Streptomyces spp. The comparison of unique genomic features of the two strains will help to explain their diverse biocontrol and plant growth-promoting activities and warrant targeted functional genomics approaches to verify their mechanisms of action.
Identification of ALP+/CD73+ defining markers for enhanced osteogenic potential in human adipose-derived mesenchymal stromal cells by mass cytometry
Authors: Canepa Daisy D. , Casanova Elisa A. , Arvaniti Eirini , Tosevski Vinko , Märsmann Sonja , Eggerschwiler Benjamin , Halvachizadeh Sascha , Buschmann Johanna , Barth André A. , Plock Jan A. , Claassen Manfred , Pape Hans-Christoph , Cinelli Paolo 
Published: January 2021

AbstractBackgroundThe impressive progress in the field of stem cell research in the past decades has provided the ground for the development of cell-based therapy. Mesenchymal stromal cells obtained from adipose tissue (AD-MSCs) represent a viable source for the development of cell-based therapies. However, the heterogeneity and variable differentiation ability of AD-MSCs depend on the cellular composition and represent a strong limitation for their use in therapeutic applications. In order to fully understand the cellular composition of MSC preparations, it would be essential to analyze AD-MSCs at single-cell level.MethodRecent advances in single-cell technologies have opened the way for high-dimensional, high-throughput, and high-resolution measurements of biological systems. We made use of the cytometry by time-of-flight (CyTOF) technology to explore the cellular composition of 17 human AD-MSCs, interrogating 31 markers at single-cell level. Subcellular composition of the AD-MSCs was investigated in their naïve state as well as during osteogenic commitment, via unsupervised dimensionality reduction as well as supervised representation learning approaches.ResultThis study showed a high heterogeneity and variability in the subcellular composition of AD-MSCs upon isolation and prolonged culture. Algorithm-guided identification of emerging subpopulations during osteogenic differentiation of AD-MSCs allowed the identification of an ALP+/CD73+ subpopulation of cells with enhanced osteogenic differentiation potential. We could demonstrate in vitro that the sorted ALP+/CD73+ subpopulation exhibited enhanced osteogenic potential and is moreover fundamental for osteogenic lineage commitment. We finally showed that this subpopulation was present in freshly isolated human adipose-derived stromal vascular fractions (SVFs) and that could ultimately be used for cell therapies.ConclusionThe data obtained reveal, at single-cell level, the heterogeneity of AD-MSCs from several donors and highlight how cellular composition impacts the osteogenic differentiation capacity. The marker combination (ALP/CD73) can not only be used to assess the differentiation potential of undifferentiated AD-MSC preparations, but also could be employed to prospectively enrich AD-MSCs from the stromal vascular fraction of human adipose tissue for therapeutic applications.
Assessing the accuracy of record linkages with Markov chain based Monte Carlo simulation approach
Authors: Haque Shovanur , Mengersen Kerrie , Stern Steven 
Published: January 2021

AbstractRecord linkage is the process of finding matches and linking records from different data sources so that the linked records belong to the same entity. There is an increasing number of applications of record linkage in statistical, health, government and business organisations to link administrative, survey, population census and other files to create a complete set of information for more complete and comprehensive analysis. To make valid inferences using a linked file, it has become increasingly important to have effective and efficient methods for linking data from different sources. Therefore, it becomes necessary to assess the ability of a linking method to achieve high accuracy or to compare between methods with respect to accuracy. This motivates the development of a method for assessing the linking process and facilitating decisions about which linking method is likely to be more accurate for a particular linking task. This paper proposes a Markov Chain based Monte Carlo simulation approach, MaCSim for assessing a linking method and illustrates the utility of the approach using a realistic synthetic dataset received from the Australian Bureau of Statistics to avoid privacy issues associated with using real personal information. A linking method applied by MaCSim is also defined. To assess the defined linking method, correct re-link proportions for each record are calculated using our developed simulation approach. The accuracy is determined for a number of simulated datasets. The analyses indicated promising performance of the proposed method MaCSim of the assessment of accuracy of the linkages. The computational aspects of the methodology are also investigated to assess its feasibility for practical use.
Penetration of the electric fields of the geomagnetic sudden commencement over the globe as observed with the HF Doppler sounders and magnetometers
Authors: Kikuchi Takashi , Chum Jaroslav , Tomizawa Ichiro , Hashimoto Kumiko K. , Hosokawa Keisuke , Ebihara Yusuke , Hozumi Kornyanat , Supnithi Pornchai 
Published: January 2021

AbstractUsing the HF Doppler sounders at middle and low latitudes (Prague, Czech Republic; Tucuman, Argentina; Zhongli, Republic of China, and Sugadaira, Japan), we observed the electric fields of the geomagnetic sudden commencement (SC) propagating near-instantaneously (within 10 s) over the globe. We found that the electric fields of the preliminary impulse (PI) and main impulse (MI) of the SC are in opposite direction to each other and that the PI and MI electric fields are directed from the dusk to dawn and dawn to dusk, respectively, manifesting the nature of the curl-free potential electric field. We further found that the onset and peak of the PI electric field are simultaneous on the day and nightsides (0545, 1250, 1345 MLT) within the resolution of 10 s. With the magnetometer data, we confirmed the near-instantaneous development of the ionospheric currents from high latitudes to the equator and estimated the location of the field-aligned currents that supply the ionospheric currents. The global simultaneity of the electric and magnetic fields does not require the contribution of the magnetohydrodynamic waves in the magnetosphere nor in the F-region ionosphere. The global simultaneity and day–night asymmetry of the electric fields are explained with the ionospheric electric potentials transmitted at the speed of light by the TM0 mode waves in the Earth-ionosphere waveguide.
Antiglycation potential of Indigoferin a, Indigoferin B and Indigoferin C natural products from Indigofera heterantha Brandis
Authors: Khan Ayesha , Khan Ajmal , Ahmad Manzoor , Ali Mumtaz , Farooq Umar , Khan Farhan Ahmad , Bukhari Syed Majid 
Published: January 2021

Abstract
Background
Diabetes is a long-lasting and serious disease that effect in worldwide individual lives, families, and societies. Hyperglycemia of diabetes mellitus produced Advance Glycation End Products that are associated with diabetic complications like neuropathy, nephropathy, retinopathy, and cardiovascular diseases.

Methods
In this study, the natural products isolated from of Indigofera heterantha Brandis, Indigoferin A (S1), Indigoferin B (S2) and Indigoferin C (S3) were evaluated for their in vitro antiglycation activity.

Results
The compounds exhibited a significant inhibitory activity against the formation of Advanced Glycation End-Products with IC50 values of 674.25 ± 3.2 μM, 407.03 ± 4.7 μM and 726.41 ± 2.1 μM, respectively. Here, important structure-activity relationship was observed, when the intramolecular hydrogen bonding interactions suppressed the antiglycation activity of compound S3. Thus, the study clearly demonstrates that the number and the position of substituents act as an assisting factor and directly influence the inhibitory activity of the natural product by altering the sugar or protein binding affinity.

Conclusions
This study explain first time the antiglycation inhibitory ability of chemical constituents isolated from I. heterantha and can be used for above late diabetic complications.
Perioperative abnormal electroencephalography in a later-stage elderly with septic shock: a case report
Authors: Kinoshita Hirotaka , Kushikata Tetsuya , Takekawa Daiki , Hirota Kazuyoshi 
Published: January 2021

Abstract
Background
Patients with sepsis often exhibit abnormal patterns of electroencephalogram (EEG). We report an abnormal EEG pattern in a later-stage elderly patient with septic shock and EEG analysis results.

Case presentation
An 88-year-old woman with bowel perforation underwent emergency Hartmann surgery. On admission to the operating room, she exhibited septic shock. Her bispectral index value was 30 before anesthesia induction, and the EEG displayed slow waves without burst and suppression throughout the surgery. The relative slow-wave ratio [spectral power (0.5–8 Hz)/(0.5–30 Hz)] from anesthetic induction to the end of surgery was 95.1%, whereas the relative alpha frequency [spectral power (8–13 Hz)/(0.5–30 Hz)] was only 2.4%. Although without preoperative neurological abnormalities, she developed postoperative delirium after admission to the intensive care unit.

Conclusions
Intraoperative continuous EEG monitoring in elderly patients with sepsis may be useful to predict sepsis-associated encephalopathy. Therefore, continuous EEG monitoring may improve neurological outcomes.
TDP-43 mislocalization drives neurofilament changes in a novel model of TDP-43 proteinopathy
Authors: Atkinson Rachel , Leung Jacqueline , Bender James , Kirkcaldie Matthew , Vickers James , King Anna 
Published: January 2021

ABSTRACT
Mislocalization of the TAR DNA-binding protein 43 (TDP-43; encoded by TARDBP) from the nucleus to the cytoplasm is a common feature of neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The downstream in vivo cellular effects of this mislocalization are not well understood. To investigate the impact of mislocalized TDP-43 on neuronal cell bodies, axons and axonal terminals, we utilized the mouse visual system to create a new model of TDP-43 proteinopathy. Mouse (C57BL/6J) retinal ganglion cells (RGCs) were transduced with GFP-tagged human wild-type TDP-43 (hTDP-WT-GFP) and human TDP-43 with a mutation in the nuclear localization sequence (hTDP-ΔNLS-GFP), to cause TDP-43 mislocalization, with ∼60% transduction efficiency achieved. Expression of both hTDP-WT-GFP and hTDP-ΔNLS-GFP resulted in changes to neurofilament expression, with cytoplasmic TDP-43 being associated with significantly (P&lt;0.05) increased neurofilament heavy expression in the cell soma, and both forms of altered TDP-43 leading to significantly (P&lt;0.05) decreased numbers of neurofilament-positive axons within the optic nerve. Alterations to neurofilament proteins were associated with significantly (P&lt;0.05) increased microglial density in the optic nerve and retina. Furthermore, expression of hTDP-WT-GFP was associated with a significant (P&lt;0.05) increase in pre-synaptic input into RGCs in the retina. The current study has developed a new model that allows detailed examination of alterations to TDP-43 and will contribute to the knowledge of TDP-43-mediated neuronal alterations and degeneration.
Structural Characterization of Y29F Mutant of Thermoglobin from a Hyperthermophilic Bacterium Aquifex aeolicus
Authors: Muraki Norifumi , Takeda Kouta , Nam Dayeon , Muraki Megumi , Aono Shigetoshi 
Published: January 2021

Abstract
We have determined the crystal structure of thermoglobin (AaTgb) from a hyperthermophilic bacterium Aquifex aeolicus. Tyrosine and glutamine at the B10 and E7 position, respectively, are conserved in AaTgb as are the case of single domain hemoglobins (sdHbs). While the binding affinity of O2 or CO is affected by the replacement of Tyr29 in the distal heme pocket, wild type and Y29F variant of AaTgb show a similar binding affinity of imidazole.
Covert cases of Severe Acute Respiratory Syndrome Coronavirus 2: An obscure but present danger in regions endemic for Dengue and Chikungunya viruses
Authors: Stringari Lorenzzo Lyrio , de Souza Michel Norbim , de Medeiros Junior Nésio Fernandes , Goulart Jaqueline Pegoretti , Giuberti Camila , Dietze Reynaldo , Ribeiro-Rodrigues Rodrigo 
Published: January 2021

Background
The impact of SARS-CoV-2 in regions endemic for both Dengue and Chikungunya is still not fully understood. Considering that symptoms/clinical features displayed during Dengue, Chikungunya and SARS-CoV-2 acute infections are similar, undiagnosed cases of SARS-CoV-2 in co-endemic areas may be more prevalent than expected. This study was conducted to assess the prevalence of covert cases of SARS-CoV-2 among samples from patients with clinical symptoms compatible with either Dengue or Chikungunya viral infection in the state of Espírito Santo, Brazil.

Methods
Presence of immunoglobulin G (IgG) antibody specific to SARS-CoV-2 nucleoprotein was detected using a chemiluminescent microparticle immunoassay in samples from 7,370 patients, without previous history of COVID-19 diagnosis, suspected of having either Dengue (n = 1,700) or Chikungunya (n = 7,349) from December 1st, 2019 to June 30th, 2020.

Findings
Covert cases of SARS-CoV-2 were detected in 210 (2.85%) out of the 7,370 serum samples tested. The earliest undiagnosed missed case of COVID-19 dated back to a sample collected on December 18, 2019, also positive for Dengue Virus. Cross-reactivity with either Dengue virus or other common coronaviruses were not observed.

Interpretation
Our findings demonstrate that concomitant Dengue or Chikungunya outbreaks may difficult the diagnosis of SARS-CoV-2 infections. To our knowledge, this is the first study to demonstrate, with a robust sample size (n = 7,370) and using highly specific and sensitive chemiluminescent microparticle immunoassay method, that covert SARS-CoV-2 infections are more frequent than previously expected in Dengue and Chikungunya hyperendemic regions. Moreover, our results suggest that SAR-CoV-2 cases were occurring prior to February, 2020, and that these undiagnosed missed cases may have contributed to the fast expansion of SARS-CoV-2 outbreak in Brazil. Data presented here demonstrate that in arboviral endemic regions, SARS-CoV-2 infection must be always considered, regardless of the existence of a previous positive diagnosis for Dengue or Chikungunya.
Implementasi Patient Safety di Pelayanan Anak Rumah Sakit Umum Haji Medan Tahun 2019
Authors: Simamora Verawaty Sari , Zulfendri Zulfendri , Simamora Roymond H , Asyura Nasution Puteri Citra Cinta 
Published: January 2021

The complexity of diversity, relationships, variety and specialization can provide more opportunities for mistakes, one of which is in the children's hospital services. Based on patient safety incident report data at Rumah Sakit Umum Haji Medan in January 2018 to October 2018, it is known that child care is the unit with the highest number of patient safety incidents compared to other units at 37 incidents. Implementation of patient safety by officers in children's services is the main focus that must be considered its role to prevent the occurrence of patient safety incidents. This research is a qualitative research that aims to see the extent of the implementation of patient safety in child care at Rumah Sakit Umum Haji Medan from the description of the role of health workers involved in child care, namely the role of the head of a child's SMF, the role of a pediatrician, and the role of a child nurse. Data collection was carried out by in-depth interviews with 7 informants and through observation. The results showed the implementation of patient safety in children's services has not been running optimally. This is because not all health workers in child services, namely the head of the child's SMF, pediatricians and child nurses do their part in the patient safety system. The roles carried out are still focused on the standards of each profession. It is expected that routine socialization on the implementation of patient safety, the implementation of special meetings and discussions to study the patient safety system and the existence of patient safety drivers in child care designated as the person responsible for moving every officer to implement patient safety. Keywords: Implementation, Children's Services, Patient Safety
Praktik Bermusik Musisi Muda dalam Skena Metal Ekstrem
Authors: Sutopo Oki Rahadianto , Lukisworo Agustinus Aryo 
Published: January 2021

This article investigates the young extreme metal musicians’ musical practices in Yogyakarta within the context of neoliberal era. To construct an empirical knowledge based on young extreme metal musicians’ narratives, this research utilized qualitative approach, specifically ethnography, from April to August 2019. In order to articulate conflictual and hierarchical dimension of extreme metal scene, this research applied Bourdieusian perspective. Based on data analysis, this study shows that, firstly, objectively, young musicians’ practices started within the condition which social inequality already existed. This represent a hierarchical characteristic of extreme metal scene as a field of struggle -ranging from local, national, to global level. Secondly, theoretically, young musicians’ practices within extreme metal scene require and reproduce particular capital, with regard to the specific rule of the game of the extreme metal scene, defined as extreme metal capital. Thirdly, the goal of the extreme metal, social, and symbolic capital accumulation, also cultural codes embodiment, was on the extreme metal scene’ doxa reproduction mechanism which accentuate three important values, namely authenticity, independency, and community. Finally, this three findings are the manifestation of extreme metal scene resistance towards state and market power in the neoliberal era. Tulisan ini berfokus pada praktik bermusik musisi metal ekstrem muda di Yogyakarta dalam konteks era neoliberal. Guna membangun pengetahuan sesuai dengan narasi musisi metal ekstrem muda, penelitian ini menggunakan pendekatan kualitatif, secara spesifik etnografi, antara April hingga Agustus 2019. Untuk mengartikulasikan dimensi konfliktual dan hierarkisitas skena metal ekstrem, penelitian ini menggunakan perspektif Bourdieusian. Adapun hasil analisis temuan lapangan menunjukkan bahwa, pertama, secara objektif, praktik bermusik musisi muda dimulai dalam kondisi dimana kesenjangan sosial telah eksis bahkan sebelum praktik tersebut terjadi. Hal ini menunjukkan karakteristik skena metal ekstrem sebagai ranah perjuangan yang bersifat hierarkis -terbentang dari leval lokal, nasional hingga global. Kedua, secara teoritis, praktik bermusik musisi muda dalam skena metal ekstrem membutuhkan dan mereproduksi kapital yang sangat spesifik terkait dengan rule of the game dalam skena tersebut, kami menyebutnya sebagai ‘kapital metal ekstrem’. Ketiga, muara dari proses akumulasi kapital metal ekstrem, kapital sosial dan kapital simbolik, serta proses embodiment mengenai kode-kode budaya terletak pada mekanisme reproduksi doxa skena metal ekstrem yang mengutamakan tiga nilai penting yaitu otentisitas, kemandirian dan komunitas. Adapun ketiga temuan tersebut merupakan manifestasi dari perlawanan skena metal ekstrem terhadap kuasa Negara dan pasar dalam era neoliberal
A DIVERSIDADE DE PRÁTICAS PRODUTIVAS DE FAMÍLIAS AGROEXTRATIVISTAS NA AMAZÔNIA
Authors: Felizardo Alciene Oliveira , Rocha Carla Giovana Souza 
Published: January 2021

O presente trabalho visa caracterizar as práticas produtivas de famílias agroextrativistas amazônicas, evidenciando o processo de inserção da criação de aves proporcionado pela participação em projeto local. Para isso foram entrevistadas onze famílias das Ilhas do Capim, Caripetuba e Xingu, município de Abaetetuba no Pará. O estudo identificou que as famílias agroextrativistas das Ilhas do Capim, Caripetuba e Xingu são heterogêneas no que se refere à disponibilidade de bases de recursos (sociais e materiais), composição, organização e funcionamento do sistema família-estabelecimento. As famílias agroextrativistas foram enquadradas em três tipos: tipo I, com base restrita de recurso material com predominância de renda externa, tipo II, com base de recurso material com média diversidade e renda centrada no extrativismo do açaí, peixe e camarão e, tipo III, com base de recurso material com alta diversidade e múltiplas fontes de renda. Isso revela que as famílias agroextrativistas beneficiárias do projeto local desenvolvem práticas distintas orientadas pelas particularidades do sistema família-estabelecimento que implicam na permanência ou resistência diante de projetos desenvolvimento agrícola padronizados.
RELAÇÕES ÉTNICO-RACIAIS E ENSINO DE HISTÓRIA NO BRASIL: O NEGRO NO SABER HISTORIOGRAFICO E SABER HISTÓRICO ESCOLAR
Authors: Júnior Elton Luis da Silva , Farias Silviane De Carvalho 
Published: January 2021

Este estudo objetiva investigar as Relações Étnico-Raciais no contexto do saber historiográfico e saber histórico escolar no ensino de História. Para tanto, buscamos identificar as Relações Étnico-Raciais que historicamente foram demarcadas por contextos de invisibilidade, sobretudo quando relacionadas a historiografia africana e afro-brasileira. As discussões sobre as imagens dos negros nos saberes produzidos na historiografia brasileira nos remetem à análise das principais interpretações e debates historiográficos desenvolvidos sobre as observações equivocadas sobre o negro no Brasil. A pesquisa apresenta uma abordagem qualitativa com aplicação de pesquisa bibliográfica. Os resultados revelam que é necessário estabelecer um ensino de História que evidencie uma educação para as Relações Étnico-Raciais, mostrando a História do Brasil como uma sociedade multirracial e pluriétnica por meio da reversão de valores e conceitos centrados em uma perspectiva eurocêntrica que coloca o negro a margem da construção histórica e cultural do Brasil. Concluímos que desde que o ensino de História passou a ser uma disciplina escolar no Brasil o foco estrutural dela foi se modelando de acordo com determinado momento histórico, fazendo com que por muitos anos houvesse a invisibilização do negro na formação cultural do país.