bluuebunny/crossref_metadata_2025_split huggingface dataset:
Degree of Coronal Alignment Correction Can’t Predict Knee Function in Total Knee Replacement
Authors: Zhu Shibai , Chen Xi , Wang Yiou , Qian Wenwei , Peng Huiming , Wei Wei , Lin Jin , Jin Jin , Weng Xisheng 
Published: March 2020

Abstract
Background: Whether neutral alignment brings better clinical outcomes is controversial. Consideration of the preoperative knee condition of patients and some limitations of previous studies, we suggested that other index may be more important than a generic target of 0° ± 3° of a neutral axis to reflect changes in coronal alignment after TKR. The purpose of this study was to explore the relationship between alignment and functional outcome with a new grouping method and the concept of correction rate. Methods: A new grouping method was adopted to divide patients into three groups based on the degree of correction of MFTA: neutral, under and over-correction. HSS were compared among the 3 groups. In addition, we also attempt to further explore whether the concept of correction rate can predict postoperative functional score. Results: HSS showed significant improvement in all groups. There was no difference in HSS or incremental scores based on the postoperative alignment category for the degree of correction of MFTA. The correlational analyses also didn’t find any relevant results. Conclusion: TKR is a soft tissue procedure and clinical outcome depends on many factors, only reaching neutral alignment after surgery may not mean a good clinical result. Therefore, categorization of optimal coronal alignment after TKR may be impractical. But we still believe that the concept of correction rate and new grouping method are worthy of research which can reflects the preoperative knee condition and the change of coronal alignment. Perhaps it can be better used in TKR in the future.
Integrative bioinformatic characterization of driver genes and prognostic features in human gastric carcinoma
Authors: Zheng Jie , Rao Lan , Han Yiming , Ding Li , Ao Jin’e , Liu Changqing 
Published: March 2020

Abstract
Background: Gastric cancer (GC) is the second most common cause of cancer death worldwide but could be more curable if diagnosed at an earlier stage. At present, the capability to predict the efficaciousness of molecular diagnosis for GC for each patient remains elusive. The purpose of this study was to identify tumor biomarkers through systems analysis of multigene predictors exploiting the available data resource.Results: Our study showed that 61 shared DEGs were significantly involved in functions such as gastric acid secretion. COL1A1, MMP9, and SPP1 were highly connected nodes in the PPI network. Among the 61 common DEGs, 34 driver genes were identified, including CST1 and MMP9. A total of 34 drug-gene interactions were found, which involved 9 common DEGs. Moreover, CTHRC1 and INHBA were related to clinical outcome in gastric cancer patients.Conclusion: The identification of new driver genes, such as MMP9, CTHRC1, and INHBA, may contribute to the understanding of the etiology of gastric cancer and the development of individualized therapies.
Different clinical presentations of two renal transplant recipients with Coronavirus Disease 2019: a case report
Authors: Li Jing , Chen Gang , chen chao 
Published: March 2020

Abstract
Background: The Coronavirus Disease 2019 (COVID-19) caused by severe acute respiratory syndrome Coronavirus-2 has spread rapidly worldwide and disease spread is currently increasing. The clinical picture of transplant recipients and the effect of the anti-rejection immunosuppressive regimens on the clinical course of COVID-19 are lacking.Case presentation: We report two cases of COVID-19infection in renal transplant recipients with variable clinical presentations. The first patient presented with mild respiratory symptoms and a stableclinical course. The second patient had more severe clinical characteristics and presented with severe pneumonia and multi-organ failure. Both patients received a combination therapy including antiviral treatment and reducedimmunosuppressiontherapy and finally recovered.Conclusions: We report COVID-19 infectionin two renal transplant recipients with a favorable outcome but different clinical courses, which may provide a reference valuefor treating such patients. Additional data are needed to gain a better understanding of the impact of immunosuppressive therapy on the clinical presentation, severity, and outcome of COVID-19in solid organ transplant recipients.
Study on the Mechanism of Application of Eucalyptus Volatile oil in Prevention and Treatment of Burn Wound Infections in Vitro
Authors: Yang Lei , Huang Qing , Gao Xingxin , Lin Zhimin , Chen Songlin , Huang Xiaodong , Feng Jinpeng , Zhao Lili , Lin Yinghui , Hua Guangzong , Li Sheng 
Published: March 2020

Abstract
Objective To investigate working mechanism of Eucalyptus volatile oil on the prevention and treatment of burn wound infections. Methods P.a biofilm model was used to investigate the effect of Eucalyptus volatile oil on bacterial biofilm. The expression of LasI mRNA in P.a was detected by RT-PCR. Results MIC test showed that the volatile oil of Eucalyptus Urophylla in a concentration of 20% or more could exert anti-bacterial effect. However, no zone of inhibition could be observed in the neither high nor low concentration of the Eucalyptus volatile oil. Scanning electron microscopy results showed a significant delay in volatile oil groups when compared with the control group. The expression of LasI mRNA in the volatile oil group was significantly lower than that in the control group. Conclusion As Chinese medicine, the volatile oil of Eucalyptus Urophylla can affect the proliferation of P.a and biofilm formation by interfering with the expression of LasI, thus achieving the purpose of preventing and treating infection of burn patients.
Inter-scanner reproducibility of brain volumetry: influence of automated brain segmentation software
Authors: Liu Sirui , Hou Bou , Zhang Yiwei , Lin Tianye , Fan Xiaoyuan , You Hui , Feng Feng 
Published: March 2020

Abstract
Background: The inter-scanner reproducibility of brain volumetry is important in multi-site neuroimaging studies, where the reliability of automated brain segmentation (ABS) tools plays an important role. This study aimed to evaluate the influence of ABS tools on the consistency and reproducibility of the quantified brain volumetry from different scanners. Methods: We included fifteen healthy volunteers who were scanned with 3D isotropic brain T1-weighted sequence on three different 3.0 Tesla MRI scanners (GE, Siemens and Philips). For each individual, the time span between image acquisitions on different scanners was limited to one hour. All the T1-weighted images were processed with FreeSurfer v6.0, FSL v5.0 and AccuBrain ® with default settings to obtain volumetry of brain tissues (e.g. gray matter) and substructures (e.g. basal ganglia structures) if available. Cofficient of variation (CV) was calculated to test inter-scanner variability in brain volumetry of various structures as quantified by these ABS tools. Results: The mean inter-scanner CV values per brain structure among three MRI scanners ranged from 6.946% to 12.29% (mean, 9.577%) for FreeSurfer, 7.245% to 20.98% (mean, 12.60%) for FSL and 1.348% to 8.800% (mean value, 3.546%) for AccuBrain @ . In addition, AccuBrain ® and FreeSurfer achieved the lowest mean values of region-specific CV between GE and Siemens scanners (from 0.818% to 5.958% for AccuBrain ® , and from 0.903% to 7.977% for FreeSurfer), while FSL-FIRST had the lowest mean values of region-specific CV between GE and Philips scanners (from 2.603% to 16.310%). Conclusion: There is a large discrepancy in the inter-scanner reproducibility of brain volumetry when using different processing software. Image acquisition protocols and selection of ABS tool for brain volumetry quantification have impact on the robustness of results in multi-site studies.
Reliability of maternal recall of delivery and immediate newborn care indicators in Sarlahi, Nepal
Authors: Carter Emily D , Chang Karen , Mullany Luke , Khatry Subarna , LeClerq Steven , Munos Melinda , Katz Joanne 
Published: March 2020

Abstract
Background: The intrapartum period is a time of high mortality risk for newborns and mothers. Numerous interventions exist to minimize risk during this period. Data on intervention coverage are needed for health system improvement. Maternal report of intrapartum interventions through surveys is the primary source of coverage data, but they may be invalid or unreliable. Methods: We assessed the reliability of maternal report of delivery and immediate newborn care for home and health facility births in Sarlahi, Nepal. Mothers were visited as soon as possible following delivery (<72 hours) and asked to report circumstances of labor and delivery. A subset was revisited 1-24 months after delivery and asked to recall interventions received using standard household survey questions. We assessed the reliability of each indicator by comparing what mothers reported immediately after delivery against what they reported at the follow-up survey. We assessed potential variation in the reliability of maternal report by characteristics of the mother, birth event, and intervention prevalence. Results: 1502 mother/child pairs were included in the reliability study, with approximately half of births occurring at home. A higher proportion of women who delivered in facilities reported “don’t know” when asked to recall specific interventions both initially and at follow-up. Most indicators had a high observed percent agreement, but kappa values were below 0.4, indicating agreement was primarily due to chance. Only “received any injection during delivery” demonstrated high reliability among all births (kappa: 0.737). The reliability of maternal report was typically lower among women who delivered at a facility. There was no difference in reliability based on time since birth of the follow-up interview. We observed over-reporting of interventions at follow-up that were more common in the population and under-reporting of less common interventions. Conclusions: This study reinforces previous findings that mothers are unable to report reliably on many interventions within the peripartum period effectively. Household surveys which rely on maternal report, therefore, may not be an appropriate method for collecting data on coverage of many interventions during the peripartum period. This is particularly true among facility births, where many interventions may occur without the mother’s full knowledge.
Production of catechol siderophores by bacteria selected from feces of a healthy female adult through screening NRPS A domain
Authors: Hu Huiling , Wu Yihe , Zhang Minai , Zhang Qing , Wang Huan , Zhang Xichun 
Published: March 2020

Abstract
Background: Catechol siderophores, which are widespread in the human gastrointestinal tract (GIT) and play important roles in maintaining iron-nutrition balance between microorganisms and host, are small molecules with a high affinity for ferric iron and are assembled by nonribosomal peptide synthetases (NRPS). In this study, we select strains encoding NRPS A domain gene from feces of a health female adult, predict its products type, and check it out in vitro . Results: Firstly, eight bacteria were determined encoding NRPS A domain gene, and then two kind of catechol siderophores, Bacillibactin and Enterobactin, were predicted according to NRPS A domain substrate specificity. Secondly, Bacillibactin and Enterobactin were checked out in cultured medium aerobic incubated with selected strains of E. Coli, Bacillus sp. and B. Cereus. For the yield of catechol siderophores, B. cereus Gut 16 secreted highest yield of Bacillibactin and E. coli Gut 07 produce highest yield of Enterobactin. Conclusion: By presence determination and activity prediction of NRPS A domain, two siderophores, Bacillibactin and Enterobactin, were checked out finally being secreted by selected strains cultured in a aerobic medium. Further research on the potential probiotic property is necessary to affirm the application in biological industry, as well as to elucidate their mechanism in human gut. Keywords : Catechol siderophores, Enterobactin, Bacillibactin, NRPS A domain, Feces, Probiotics
Berry Raw Materials in Functional Soft Cheese Production
Authors: Borisova Anna , Ruzyanova Anna , Tyaglova Anna , Polikarpova Ksenia 
Published: March 2020

Introduction. Healthy diet has been in the focus of scientific attention for many years. This issue is closely connected with the production of functional foods. Functional food products contain functional ingredients that are beneficial to living systems. The present research features functional soft cheese based on dried berries that are rich in vitamin C, which possesses antioxidant activity, reduces the risk of blood clots, and improves the immune system.
Study objects and methods. An online survey was conducted to study the Russian cheese market. The experiments featured samples of sea-buckthorn, aronia, and rosehip berries and samples of milk whey obtained at different stages of cheese production. The sampels of the berries were analysed for physical and chemical properties, i.e. acidity, contents of dry substances, moisture, food fibers, vitamin C content, etc. Acidity of milk whey was evaluated in pH.
Results and discussion. The market analysis revealed that Russian cheese market needs to be expanded. Potential customers would agree to purchase cheese with various berry additives. Almost 30% of respondents claimed that they would like to buy cheese with sea-buckthorn berries. We produced four samples of cheese with raw material that was rich in vitamin C. A panel of six potential customers evaluated the samples for sensory properties using a five-point scale, i.e. color, taste, aroma, consistency, and shape. The sea-buckthorn cheese received the highest score: 22.3 points out of 25.
Conclusion. Powdered sea-buckthorn, aronia, and rosehip berries could be used to produce quality functional soft cheese. The research helped to define the optimal way of introducing berry additives into cheese products. Powdered berries were introduced while mixing curd, which helped to maintain whey acidity at the required level and improve the qualitaty of the final product. When the powdered berries were introduced into the milk before the enzyme, the consistency of the obtained cheese was unsatisfying: it broke down into curds, and its viscosity was low.
Rational Technological Parameters of the Soxhlet Extractor in the Production of Alcoholic Extracts from Cranberries
Authors: Fedorenko Boris , Borodulin Dmitriy , Prosin Maksim , Shafray Anton , Lobasenko Boris , Golovacheva Yana 
Published: March 2020

Introduction. Vegetable raw materials have always been and still remain the main raw material for obtaining extracts. The extraction process is the most important stage during the production of extracts and aromatic spirits. The research objective was to study the extraction process of the target substances from fruit and berry raw materials for the production of alcoholic extracts.
Study objects and methods. The study featured cranberries, which are widespread in Siberia and have many useful properties. Cranberries are rich in vitamins C, B1, B2, B5, B6, PP and rarer vitamin K1. The studies were conducted using a Soxhlet extractor. The experimental results were processed using the method of multiple regression analysis. The research also exploited generally accepted methods for assessing the quality of samples of alcoholic beverages.
Results and discussion. The experiments made it possible to define the optimal technological regime parameters for the production of aromatic cranberry extract. The maximum values of all three processing parameters exerted the greatest effect on the dry matter content in cranberry extract. The recommended conditions resulted in a cranberry extract with a high content of target components. The presented mathematical description of the extraction process in the Soxhlet extractor makes it possible to preliminarily determine the quality indicators of the resulting beverage at the onset of production. Control experiments conducted under these conditions confirmed the calculated values.
Conclusion. By using a Soxhlet extractor, the preparation time for alcoholic cranberry extract was reduced to 20 minutes, which differs by one order of magnitude from the classical infusion production method. The Soxhlet extractor made it possible to use less solvent and raw materials, due to the almost maximum completeness of extraction of the target components of fruit and berry raw materials. This fact is bound to decrease the production expenses and the cost of the final product.
Yeasts as a Glutathione Producer
Authors: Meledina Tatiana , Morozov Artyom , Davydenko Svetlana , Ternovskoy Grigoriy 
Published: March 2020

Introduction. Yeast is a fast-growing single-celled microorganism and an inexpensive source of various biologically active substances, such as antioxidants, e.g. Glutathione (GSH). Antioxidant properties are determined by the presence of sulfhydryl group. The global demand for glutathione is estimated to exceed 9 billion USD at the expense not only of pure crystalized glutathione, but also of glutathione-enriched yeast extracts. In the food industry, glutathione is used to improve the quality of the dough and enhance the taste of various products. The present research featured domestic and foreign studies on the content of glutathione in yeast, methods of biosynthesis, and antioxidant properties.
Results and discussion. The content of glutathione ranges from 0.1 to 1% per completely dry biomass (CDB) in wild yeast strains. The fermentative method for the accumulation of glutathione is based on the optimization of the nutrient medium and the use of glutathione precursors, i.e. cysteine, glutamic acid, and glycine. Thus, this method makes it possible to double the content of intracellular glutathione in certain cultivation conditions. The use of non-directed mutagenesis methods can increase glutathione synthesis up to 5% in separate mutant strains, although the mechanism of synthesis is not always clear under such conditions. However, up to 2.27% of glutathione is being formed under directed change of the genome. In addition, the level of glutathione in cells increases under the influence of certain physical factors. For example, glutathione biosynthesis increases by 39% if yeast is exposed to a magnetic field. The enzymatic method requires maintaining the following factors: the presence of precursors (L-glutamic acid, L-cysteine, glycine), ATP, Mg2+ ions to activate GSH1 and GSH2, the pH of the medium, and the introduction of the necessary enzymes into the bioreactor. Hiwever, this method is non-economically profitable in large scale productions due to the needs in use ATP.
Conclusion. The survey research demonstrated the effect of technological characteristics of cultivation and biotechnological properties of Saccharomyces cerevisiae on the accumulation of glutathione.
Ostrich Fat Production Using Electrolyzed Fluid
Authors: Gorbacheva Mariya , Tarasov Vasily , Kalmanovich Svetlana , Sapozhnikova Alla 
Published: March 2020

Introduction. The fundamental competitiveness of all food industries is based on two key points. The first one is the effective use of traditional and new raw materials. The second one is a constant upgrade of the technical base by introducing innovative technological solutions and modern equipment. In this aspect, the fat processing industry is no exception. The development of a comprehensive and sustainable processing of ostrich fat can help to obtain rendered fat with specufic properties that can be used in functional foods and cosmetics. The research objective was to improve the parameters of ostrich fat rendering by using electrolyzed fluid in order to obtain a functional fat product of high quality indicators.
Study objects and methods. The research featured ostrich fat obtained by wet melting in the aqueous phase of electrolyte (catholyte). A saline solution of sodium chloride (4 g/100cm3) was subjected to electrochemical treatment in an electrolytic bath with a direct current of 0.5–0.6 A and a voltage of 40–42 V. For fat extraction, we used catholyte with the following parameters: pH 9–11, redox potential between –600 and –700 mV. A two-factor experiment helped to improve the technological parameters of fat rendering. Catholyte hydrogen index X1 (Z1) and fat melting temperature X2 (Z2) were chosen as the primary technological parameters.
Results and discussion. The processing temperature of raw fat had a significant effect on the fat yield. When the catholyte hydrogen index and the temperature were increased, it had a negative effect on fat extraction. The acid value of ostrich fat was significantly affected by the pH of catholyte. The inactivating effect of catholytic action on the enzymatic processes resulted in low values of peroxide fat.
Conclusion. The research provided the following optimal range for producing ostrich fat with improved consumer properties: hydrogen index = 10.5–11.0, melting temperature = 60.0–100.00°C.
Encapsulation of Powdered Rowanberries (Sorbus aucuparia) with Plant Polysaccharides
Authors: Matseychik Irina , Martynova Elizaveta , Korpacheva Svetlana , Shteer Angelina , Lomovskiy Igor 
Published: March 2020

Introduction. Functional food industry has a stable tendency to use natural plant materials and products. The rowan thrives in Siberian conditions, which makes rowanberries a local raw material of plant origin. The article features the process of encapsulation of bitter plant extracts, which makes it possible to create new functional ingredients.
Study objects and methods. The plant extract was obtained from the powder of infrared-dried uncultivated rowanberries harvested in the city of Novosibirsk and the Altai Territory in September 2018 and October 2019. The research compared two parallel encapsulation technologies: spray and freeze drying. The encapsulating matrix vs. IR-dried powder ratio was established experimentally. Konjac and guar gum polysaccharides were used as encapsulating membranes. The content of antioxidants, flavonoids, vitamin C, and β-carotene was determined in the IR-dried rowanberry extract and encapsulated powder. A set of experiments made it possible to compile a new formulation of a cottage cheese dessert with encapsulated functional additive. The dessert was tested for various quality indicators that affect the physicochemical and rheological properties of the product, i.e. moisture, solids content, sugar content, vitamin C, β-carotene, antioxidant capacity, and biologically active flavonoids.
Results and discussion. Encapsulation significantly improved the sensory properties of the final material. The physicochemical quality assessment showed that a 1:1 dilution of rowan extract with a food polysaccharide did not affect the content of biologically active substances (with a tolerance of 8%). The physical and chemical quality assessment confirmed the functionality of the dessert: it contained > 15% of the daily physiological requirements of vitamin C, β-carotene, antioxidants, and flavonoids.
Conclusion. The paper introduces a novel method of rowan powder encapsulation, which was successfully applied to create a new functional product. The encapsulation technology made it possible to soften the natural bitterness of the raw material. The new functional product demonstrated excellent sensory properties and nutritional value.