bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Zika virus neutralizing antibodies in healthy adults in Vietnam during and after the Zika virus epidemic season: a longitudinal population-based survey
Authors: Nguyen Co Thach , Moi Meng Ling , Le Thi Quynh Mai , Nguyen Thi Thu Thuy , Vu Thi Bich Hau , Nguyen Hai Tuan , Pham Thi Thu Hang , Le Thi Hien Thu , Nguyen Le Manh Hung , Ly Minh Huong Phu , Ng Chris Fook Sheng , Takemura Taichiro , Morita Kouichi , Hasebe Futoshi 
Published: April 2020

Abstract
Background : Between 2016 and 2019, 265 cases of Zika virus (ZIKV) infection were reported in Vietnam, predominantly in southern Vietnam. In 2016, a case of ZIKV-associated microcephaly was confirmed in the Central Highlands, and several members of the infant’s family were confirmed to be infected with ZIKV. The study aims to determine the level of immunity to ZIKV in the general population of the ZIKV epidemic region. Methods: A total of 879 serum samples were collected from 801 participants between January 2017 and July 2018, during and after the ZIKV epidemic in Vietnam. The samples were tested for anti-ZIKV immunoglobulin M (IgM) and immunoglobulin G (IgG), and anti-dengue virus (DENV) IgG antibodies using enzyme-linked immunosorbent assays (ELISA). Plaque-reduction neutralization test (PRNT) for ZIKV was performed on all samples, and for DENV on the samples that ZIKV neutralizing antibody positive. Results: A total of 83 (10.3%) participants had anti-ZIKV IgM. Of the 83, 6 were confirmed to be ZIKV antibodies positive using PRNT and anti-ZIKV IgG ELISA. Of the 718 participants who were anti-ZIKV IgM negative, a further 3 cases were confirmed as positive for antibodies against ZIKV. Of the 9 participants with ZIKV infection, 5 lived in the same village as the infant with ZIKV-associated microcephaly and the other 4 lived in 2 neighboring communes. Repeat samples were collected from the 83 ZIKV IgM positive participants 1.5 years after the first collection. No new cases of ZIKV infection were detected. In addition, 2 of 3 participants with anti-ZIKV NS1 IgG demonstrated a 4- to 8-fold increase in ZIKV neutralizing antibody titer. Conclusions: ZIKV was present in the area around Krong Buk, with the rate of ZIKV-specific antibodies was 1.1% in the community since at least 2016. While the low levels of circulation together with low seroprevalence suggests a limited outbreak in the region, the results also reflect on low levels of protective immunity to Zika within the population. These results provide a better understanding of the current ZIKV epidemic status in the region and demonstrate a need for implementation of more effective ZIKV infection control measures.
Comparing the Effects of Ketorolac and Paracetamol on Postoperative Pain relief after Coronary Artery Bypass Graft surgery. A randomized clinical trial
Authors: Zadeh Fatemeh Javaherforoosh , Abdalbeygi Hasan , Janatmakan Farahzad , Gholizadeh Behnam 
Published: April 2020

Abstract
Introduction : Pain management after coronary artery bypass graft (CABG) surgery remains challenging. Objective This study aimed to compare the effects of Ketorolac and Paracetamol on postoperative CABG pain relief. Method: This double-blind randomized clinical trial study was conducted in Ahvaz, Iran, from September 2018- December 2019. Two consecutive groups of 60 patients undergoing elective on-pump coronary artery bypass graft surgery. Intervention The patients were divided into 0.5 mg/kg of ketorolac mg/dl and 10 mg/kg of Paracetamol after surgery for pain management . Primary outcomes were: visual analog pain scale (VAS) at the time point immediately after extubation (baseline) and at 6, 12, 24 and 48 hours and the total dose of morphine consumption. Secondary outcomes included the hemodynamic variables, weaning time, chest tube derange, in-hospital mortality and myocardial infarction. Statistical analysis: The data were analyzed using SPSS version 22(SPSS, Chicago, IL). The Mann-Whitney U-test was used to compare demographic data, VAS scores, vital signs, and side effects. Repeated measurements were tested within groups using Friedman's ANOVA and the Wilcoxon rank-sum test. Values were expressed as means ± standard deviations. Statistical significance was defined as a p-value < 0.05. Results : Compared with baseline scores, there were significant declines in VAS scores in both groups throughout the time sequence (P＜ 0.05). The statistical VAS score was slightly higher in the Paracetamol group at most time points, except for the time of 6 h. However, at 24 and 48 hours, the VAS score in group Paracetamol was significantly higher than in group Ketorolac. There were no significant differences between groups about hemodynamic variables. Conclusion: The efficacy of ketorolac is comparable to that of Paracetamol in postoperative CABG pain relief. Trial registry: IRCT20150216021098N5. Registered at 2019-09-12
Trauma care and capture rate of variables of World Health Organisation data set for injury at regional hospitals in Tanzania: first steps to a national trauma registry
Authors: Sawe Hendry Robert , Reynolds Teri A. , Weber Ellen J. , Mfinanga Juma A. , Coats Timothy J. , Wallis Lee A. 
Published: April 2020

Abstract
Background: In Tanzania, there is no national trauma registry. The World Health Organization (WHO) has developed a data set for injury that specifies the variables necessary for documenting the burden of injury and patient-related clinical processes. As a first step in developing and implementing a national Trauma Registry, we determined how well hospitals currently capture the variables that are specified in the WHO injury set. Methods: This was a prospective, observational cross-sectional study of all trauma patients conducted in the Emergency Units of five regional referral hospitals in Tanzania from February 2018 to July 2018. Research assistants observed the provision of clinical care in the EU for all patients, and documented performed assessment, clinical interventions and final disposition. Research assistants used a purposefully designed case report form to audit the injury variable capture rate, and to review Ministry of Health (MoH) issued facility Register book recording the documentation of variables. We present descriptive statistics for hospital characteristics, patient volume, facility infrastructure, and capture rate of trauma variables.Results: During the study period, 2,891 (9.3%) patients presented with trauma-related complaints, 70.7% were male. Overall, the capture rate of all variables was 33.6%. Documentation was most complete for demographics 71.6%, while initial clinical condition, and details of injury were documented in 20.5% and 20.8% respectively. There was no documentation for the care prior to Emergency Unit arrival in all hospitals. 1430 (49.5%) of all trauma-related visits seen were documented in the facility Health Management Information System register submitted to the MoH. Among the cases reported in the register book, the date of EU care was correctly documented in 77% cases, age 43.6%, diagnosis 66.7%, and outcome in 38.9% cases. Among the observed procedures, initial clinical condition (28.7%), interventions at Emergency Unit (52.1%), investigations (49.0%), and disposition (62.9%) were documented in the clinical charts.Conclusions: In the regional hospitals of Tanzania, there is inadequate documentation of the minimum trauma variables specified in the WHO injury data set. Reasons for this are unclear, but will need to be addressed in order to improve documentation to inform a national injury registry.
Data Driven Decision Making to Characterize Clinical Personas of Parents of Children with Cystic Fibrosis: A Mixed Methods Study
Authors: Szczesniak Rhonda , Pestian Teresa , Duan Leo L , Li Dan , Stamper Sophia , Ferrara Brycen , Kramer Elizabeth , Clancy John P , Grossoehme Daniel 
Published: April 2020

Abstract
Background: Beginning at a young age, children with cystic fibrosis (CF) embark on demanding care regimens that pose challenges to parents. We examined the extent to which clinical, demographic and psychosocial features inform patterns of adherence to pulmonary therapies and how these patterns can be used to develop clinical personas, defined as aspects of adherence barriers that are presented by parents and/or perceived by clinicians, in order to enhance personalized CF care delivery. Methods: We undertook an explanatory sequential mixed-methods study consisting of i) multivariate clustering to create clusters corresponding to parental adherence patterns (quantitative phase); ii) parental participant interviews to create clinical personas interpreted from clustering (qualitative phase). Clinical, demographic and psychosocial features were used in supervised clustering against clinical endpoints, which included adherence to airway clearance and aerosolized medications and self-efficacy score, which was used as a feature for modeling adherence. Clinical implications were developed for each persona by combing quantitative and qualitative data (integration phase). Results: The quantitative phase showed that the 87 parent participants were segmented into three distinct patterns of adherence based on use of aerosolized medication and practice of airway clearance. Patterns were primarily influenced by self-efficacy, distance to CF care center and child BMI percentile. The two key patterns that emerged for the self-efficacy model were most heavily influenced by distance to CF care center and child BMI percentile. Eight clinical personas were developed in the qualitative phase from parent and clinician participant feedback of latent components from these models. Findings from the integration phase include recommendations to overcome specific challenges with maintaining treatment regimens and increasing support from social networks. Conclusions: Adherence patterns from multivariate models and resulting parent personas with their corresponding clinical implications have utility as clinical decision support tools and capabilities for tailoring intervention study designs that promote adherence. Trial registration: Not applicable.
Establishing a diagnostic scale of subacute thyroiditis without radioisotope scanning
Authors: Xiong Zhouyi , Luo Chunying , Wang Li , Xiong Bin , Wu Jianneng 
Published: April 2020

Abstract
Background: Radioisotope scanning is important to diagnose subacute thyroiditis(SAT), but it’s not always available. So we aim to establish a diagnostic scale for SAT without radioisotope scanning.Methods: The suspected SAT patients hospitalized in Yuebei people's Hospital from January 2012 to December 2016 were selected and divided into study group and control group according to whether they were diagnosed as SAT. The clinical indexes of two groups were collected and the diagnostic scale of SAT was established by using binary logistic regression analysis. The effectiveness of the scale was evaluated by ROC curve. Results: Of 309 patients, 58.25% of patients were confirmed with SAT and the remaining 41.75% of patients were not diagnosed with SAT. After univariate analysis, variables which were considered statistically different(P<0. 05) between the two groups were selected as independent variables and the diagnosis of SAT was taken as dependent variable in the binary logistic regression model. The logistic regression model consisted of 4 variables, each was thyroid tenderness, firm on palpation, increased ESR and elevated thyroid hormone level. The P value of Omnibus tests was≤0. 001 and the Nagelkerke R Square was 0. 915. The diagnostic scoring scale was established with these four variables according to their regression coeffient. The area under the ROC curve for this diagnostic scale was 0. 991(95% confidence interval, 0. 982-0.999). The highest Youden index was 0. 912, the corresponding cut-off point was 7. Internally validation shows a sensitivity of 92. 78% and a specificity of 98.45% of our scale. Conclusions: We established and validated a diagnostic scale for SAT without the need for radioisotope scanning for the first time. It has good application in institutions that do not have radioisotope machines or among pregnant and lactating women.
Legislation, pollution parameters and biologically active substance concentrations: evaluating origin of sapropel for pharmaceautical production
Authors: Pavlovska Ilona , Klavina Aneka , Auce Agris , Vanadzins Ivars , Silova Alise , Komarovska Laura , Silamikele Baiba , Dobkevica Linda , Paegle Linda 
Published: April 2020

Abstract
Background

Development trends need the necessity for wider use of the local resources and available natural materials are one of the priorities around the world. Freshwater sapropel is a common material in the water basement of the lakes in Latvia, but still not sufficiently explored. The main goal of the project to start detailed and systematic research on the medical properties of sapropel to be obtained in Latvia, promote its scientifically based use in balneology, develop new medical procedures and services, and promote development of new exportable products.
Results

The results include the survey, sampling depths and processing, evaluation of external signs, physical, chemical and biochemical parameters and evaluation of microbiological indicators. Active components from the sapropel samples extracted using the alkaline method. Sapropel extracts were characterized by organic carbon content, humic and fulvic acid concentrations, total phenolic content, trace metal and pesticide concentrations, total antioxidant status and microbiological flora.
Conclusions

Summarizing the article's main findings it was concluded that Latvian freshwater sapropel can be used as raw material for obtaining sapropel extract and use it in the preparation of pharmaceuticals and promote the development of new exportable products and services.
Predicting the COVID-19 Prevalence Rate Using Data Mining
Authors: Ahouz Fatemeh , golabpour amin 
Published: April 2020

Abstract
Background The high prevalence COVID-19 has made it a new pandemic. Predicting the prevalence and incidence of this disease throughout the world is crucial to helping health professionals make key decisions about the disease.Methods The coronavirus dataset contains information on COVID-19 cases in 252 geographic regions since January 22 and is updated daily. Data are included in the analysis as of March 29, 2020, with 17,136 records and 4 variables: latitude, longitude, date, and records. In order to design the prevalence pattern for each geographic area, the information of the region and its neighborhoods in the past two weeks, has been used. Then, using a Boosting Classification algorithm, a method was developed to predict the prevalence rate for the next two weeks.Results The model was presented for three groups with a prevalence of less than 200, ranging from 200 to 1000, and more than 1000 cases, and the model error rates were 9.42, 17.08, and 12.26, respectively. In addition, more than 1 million new cases are expected to become infected in the next two weeks (March 30 - April 12). The number of new cases are expected to be more than 19,000 new cases in Africa, over 100,000 in Asia, over 14,000 in Australia, over 600,000 in Europe and over 300,000 in the Americas.Conclusion The increase in the prevalence growth rate of the disease will also be in the Southern Hemisphere, and the United States will have the highest prevalence worldwide.
Inhibition of mTOR-Akt-ULK1 signaling pathway and activation of autophagy by TNFAIP8 in metastasis of gastric cancer
Authors: Chen Zheng , Zhang Jianguo , Dong Chenyang , Li Dongsheng , Yin Yuehan , Yu Wenhai , Chen Yuezhi 
Published: April 2020

Abstract
Background

The purpose of this article is to study the mechanism of TNFAIP8 in gastric cancer.
Methods

RT-PCR was used to detect the expression of TNFAIP8 mRNA in normal gastric mucosa cells and four gastric cancer cell lines. TNFAIP8 was silenced or overexpressed in two cell lines, CCK-8 cell viability was used, transwell experiment was used to detect cell invasion capability, and flow cytometry was used to detect cell apoptosis. TNFAIP was silenced or overexpressed in a cell line, and nude mice were inoculated to form transplanted tumors. HE staining and immunohistochemistry staining were used to detect the histopathological changes of tumors.
Results

The expression of TNFAIP8 was significantly up-regulated in GC patients and cells. After silencing and overexpressing TNFAIP8, gastric cancer cells with high expression increased, apoptosis decreased, and cell invasion increased. Expression of mTOR-Akt-ULK1 signal pathway was inhibited and autophagy signal was activated.
Conclusions

Our findings indicate that TNFAIP8 inhibits the metastasis of gastric cancer cells by inhibiting mTOR-Akt-ULK1 signal pathway and activating autophagy signal.
Non-Intubated Anesthesia Video-Assisted Thoracic Surgery for Subxiphoid Anterior Mediastinal Tumor Resection
Authors: Liang Hengrui , Qiu Yuan , Deng Shiqi , Li Shuben , Wang Wei , Weixiang Lu. , Zhou Yanran , Zhang Yaoliang , Wu Xi , Liang Wenhua , Jiang Long , Chen Hanzhang , He Jianxing 
Published: April 2020

Abstract

Objective

Subxiphoid approach for mediastinal tumor resection was reported to provide a better view and less postoperative pain. Non-intubated video-assisted thoracic surgery (NI-VATS) without muscle relaxant would decrease the possibility of postoperative airway collapse for anterior mediastinal mass operation. Herein, we sought to describe the use of NI-VATS through subxiphoid approach for anterior mediastinal tumor resection.
Methods

Patients that underwent subxiphoid VATS resection for anterior mediastinal tumor between December 2015 and September 2019 were retrospectively included for analysis. Patients were divided into two groups according to airway management: NI-VATS and intubated VATS (I-VATS). Intraoperative and postoperative variables were compared.
Results

A total of 40 patients were included. Among them, 21 patients received NI-VATS (52.5%) and 19 were treated with I-VATS (47.5%). In total, intraoperative (4/21 vs. 2/19; p = 0.446) and postoperative complications (5/21 vs. 7/19; p = 0.369) were similar between NI-VATS and I-VATS group. The anesthesia time (231.76 vs 244.71 min; p = 0.218), the operation time (152.35 vs 143.64 min; p = 0.980) and the length of stay (9.47 vs 10.57 day; p = 0.970) were similar between the two groups. Chest tube duration was shorter in NI-VATS groups (1.81 vs 1.84 day; p = 0.008), however, the total volume (351.95 vs 348.00 ml; p = 0.223) was similar. The post-operative pain scores (2.79 vs 2.93, P = 0.413) were comparable between two groups.
Conclusions

NI-VATS for mediastinal tumor resection via subxiphoid approach is a safe and technically feasible option. This technique leads to comparable perioperative clinical outcomes when compared with I-VATS via subxiphoid approach.
Genotype-Phenotype Correlation of HbH Disease in Northern Iraq
Authors: Shamoon Rawand P , Yassin Ahmed K , Polus Ranan K , DaherAli Mohamed D 
Published: April 2020

Abstract
Background: HbH disease results from dysfunction of three, less commonly two, α-globin genes through various combinations of deletion and non-deletion mutations. Characterization of the mutations and the underlying genotypes is fundamental for proper screening and prevention of thalassaemia in any region. The aim of this study was to explore the genetic arrangements of HbH disease and to correlate the genotypes with the clinical phenotypes.Method: A total of 44 HbH disease patients were enrolled in this study. They were clinically and haematologically assessed. The patients were tested for 21 common α-globin gene mutations using multiplex PCR and reverse hybridization. According to the genotype, the patients were categorized into two separate sub-groups, deletion and non-deletion types HbH disease. Results: Within the studied HbH disease patients, eight different α-globin gene mutations were detected in nine different genetic arrangements. The --MED and -α3.7 deletions were the two most frequently encountered mutations (37.5% and 35.2% respectively). Patients with deletion genotypes constituted 70.4%. The most common detected genotype was --MED/-α3.7 (59.1%), followed by αpoly-A1α/αpoly-A1α (13.6%). For the first time, coinheritance of two relatively mutations (-α3.7/ααAdana) was unpredictably detected in a 1.5 year-old child with Hb of 7.1 g/dL. Conclusion: The HbH disease patients’ clinical characteristics were variable with no ample difference between the deletion and non-deletion types. These results can be of benefit for the screening and management of thalassaemia in this region.
Epidemiological and Clinical Features of 2019 Novel Coronavirus Diseases (COVID-19) in the South of Iran
Authors: Shahriarirad Reza , Khodamoradi Zohre , Erfani Amirhossein , pour Hamid reza Hossein , Ranjbar Keivan , Emami Yasaman , Mirahmadizadeh Alireza , Lotfi Mehrzad , Yeganeh Babak Shirazi , Nejad Abolfazl Dorrani , Hemmati Abdolrasool , Ebrahimi Mostafa , Moghadami Mohsen 
Published: April 2020

Abstract
Background
In March 2020, WHO has declared pandemic on COVID-19. Although the number of infected cases is increasing, information about its clinical characteristics in the Middle East especially in Iran, a country which is considered as one of the most important foci of the disease in the world is lacking. Till date, there is no available literature on the clinical data on COVID-19 patients in Iran.
Method and Material:
In this multicenter retrospectively registered study, 113 hospitalized confirmed cases of COVID-19 admitted in university affiliated hospitals in Shiraz, Iran in from February 20 to March 20 were enrolled.
Results
The median age was 53 years and 71 (62.8%) were males. The most common symptoms at onset were fatigue (75: 66.4%), cough (73: 64.6%), and fever (67: 59.3%). Laboratory data revealed significant correlation between lymphocyte count, partial thromboplastin time, international normalized ratio with the severity of the disease (P value = 0.003, 0.000, 0.000, respectively). The most common abnormality in chest CT scan was ground-glass opacity (77: 93.9%), followed by consolidation (48: 58.5%). Our results revealed an overall 8% (9 out of 113 cases) mortality rate among the patients, in which the majority was among the ICU admitted patients (5: 55.6%). Also, 68 (60.2%) of our patients achieved total recovery and 7 (6.2%) were discharged with follow-up and home isolation.
Conclusion
In this multi-center study which included 113 hospitalized patients with diagnosis of COVID-19, 9.7% of cases were transferred to ICU and mortality rate was 8%. Furthermore, finding the source of infection and studying the behavior of COVID-19 is crucial for understanding the pandemic.