bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of human telomerase reverse transcriptase–derived peptides that induce HLA-A24–restricted antileukemia cytotoxic T lymphocytes
Authors: Arai Junko , Yasukawa Masaki , Ohminami Hideki , Kakimoto Miki , Hasegawa Atsuhiko , Fujita Shigeru 
Published: July 2002

AbstractHuman telomerase reverse transcriptase (hTERT) is considered a potential target for cancer immunotherapy because it is preferentially expressed in malignant cells. hTERT-derived peptides carrying motifs for HLA-A24 (HLA-A*2402), the most common allele among Japanese and also frequently present in persons of European descent, were examined for their capacity to elicit antileukemia cytotoxic T lymphocytes (CTLs). Two of the 5 peptides tested, VYAETKHFL and VYGFVRACL, appeared capable of generating hTERT peptide-specific and HLA-A24–restricted CTLs. The CD8+ CTL clones specific for these hTERT peptides exerted cytotoxicity against leukemia cells in an HLA-A24–restricted manner. This cytotoxicity was inhibited by the addition of hTERT peptide-loaded autologous cells, suggesting that hTERT is naturally processed in leukemia cells and that hTERT-derived peptides are expressed on these cells and are recognized by CTLs in the context of HLA-A24. Taken together with the currently identified HLA-A2–restricted CTL epitopes derived from hTERT, identification of new CTL epitopes presented by HLA-A24 increases the feasibility of immunotherapy for leukemia using hTERT-derived peptides.
Expression of tumor necrosis factor–related apoptosis-inducing ligand, Apo2L, and its receptors in myelodysplastic syndrome: effects on in vitro hemopoiesis
Authors: Zang Dae Young , Goodwin Ray G. , Loken Michael R. , Bryant Eileen , Deeg H. Joachim 
Published: July 2002

AbstractTumor necrosis factor–related apoptosis-inducing ligand (TRAIL), a member of the tumor necrosis factor (TNF) family, binds to several cell-surface receptors with distinct functions (agonistic receptors 1 and 2 [TRAIL-R1, TRAIL-R2]; decoy receptors 3 and 4 [TRAIL-R3, TRAIL-R4]). Expression and function was characterized in patients with myelodysplastic syndromes (MDSs). While normal marrow showed negligible expression of TRAIL and receptors (except TRAIL-R3), TRAIL and all receptors were constitutively expressed in MDS marrow. Following TRAIL exposure, MDS marrow showed significant increases in apoptosis, whereas normal marrow, except for a subset of CD34+ precursors, did not (P = .012). Marrow from 21 patients with MDS was then propagated in long-term cultures in the presence or absence of TRAIL. While in advanced MDS (refractory anemia with excess blasts in transformation [RAEB-T] and tAML [MDS transformed into AML]), colony numbers decreased in the presence of TRAIL (63.0% ± 10.4% of untreated group [100%]), numbers increased in patients with RA or RAEB (160.2% ± 90.5% of untreated group). TRAIL eliminated preferentially clonally abnormal cells as identified by chromosomal markers. Thus, TRAIL and receptor expression differed significantly between normal and MDS marrow, and TRAIL modulated in vitro hemopoiesis in MDS dependent upon disease stage but not, to a detectable extent, in normal marrow.
Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1
Authors: Pendl Gunther G. , Robert Caroline , Steinert Meike , Thanos Renate , Eytner Ruth , Borges Eric , Wild Martin K. , Lowe John B. , Fuhlbrigge Robert C. , Kupper Thomas S. , Vestweber Dietmar , Grabbe Stephan 
Published: July 2002

AbstractInflammatory processes are associated with the rapid migration of dendritic cells (DCs) to regional lymph nodes and depletion of these potent antigen-presenting cells (APCs) from the inflamed tissue. This study examined whether sites of cutaneous inflammation can be repopulated with DCs from a pool of immature DCs circulating in the blood. In adoptive transfer experiments with ex vivo–generated radioactively labeled primary bone marrow–derived DCs injected into mice challenged by an allergic contact dermatitis reaction, immature DCs were actively recruited from the blood to sites of cutaneous inflammation, whereas mature DCs were not. Immature, but not mature, DCs were able to adhere specifically to immobilized recombinant E- and P-selectin under static as well as under flow conditions. P-selectin–dependent adhesion of immature DCs correlates with their higher level of expression of the carbohydrate epitope cutaneous lymphocyte-associated antigen (CLA) and is blocked by a novel inhibitory antibody against mouse P-selectin glycoprotein ligand 1 (PSGL-1). Surprisingly, however, emigration of immature DCs into inflamed skin is retained in the presence of this anti–PSGL-1 antibody and is also normal when immature DCs are generated from fucosyltransferase (Fuc-T) Fuc-TVII–deficient mice. By contrast, emigration of wild-type immature DCs is reduced by adhesion-blocking anti–E- and P-selectin antibodies, and immature DCs generated ex vivo from Fuc-TVII/Fuc-TIV double-deficient mice emigrate poorly. Thus, fucosylated ligands of the endothelial selectins, determined in part by Fuc-TIV, and independent of PSGL-1, are required for extravasation of DCs into sites of cutaneous inflammation.
Hydroalkylation of Aryl Alkenes with Organohalides Catalyzed by Molybdenum Oxido Based Lewis Pairs
Authors: Zwettler Niklas , Dupé Antoine , Klokić Sumea , Milinković Angela , Rodić Dado , Walg Simon , Neshchadin Dmytro , Belaj Ferdinand , Mösch‐Zanetti Nadia C. 
Published: June 2020

AbstractThree molybdenum(VI) dioxido complexes [MoO2(L)2] bearing Schiff base ligands were reacted with B(C6F5)3 to afford the corresponding adducts [MoO{OB(C6F5)3}(L)2], which were fully characterized. They exhibit Frustrated Lewis‐Pairs reactivity when reacting with silanes. Especially, the [MoO{OB(C6F5)3}(L)2] complex with L=2,4‐dimethyl‐6‐((phenylimino)methyl)phenol proved to be active as catalyst for the hydroalkylation of aryl alkenes with organohalides and for the Atom‐Transfer Radical Addition (ATRA) of organohalides to aliphatic alkenes. A series of gem‐dichloride and gem‐dibromide compounds with potential for further derivatization were synthesized from simple alkenes and organohalides, like chloroform or bromoform, using low catalyst loading.magnified image
Differentiating molecular etiologies of Angelman syndrome through facial phenotyping using deep learning
Authors: Gomez Diego A. , Bird Lynne M. , Fleischer Nicole , Abdul‐Rahman Omar A. 
Published: June 2020

AbstractAngelman syndrome (AS) is caused by several genetic mechanisms that impair the expression of maternally‐inherited UBE3A through deletions, paternal uniparental disomy (UPD), UBE3A pathogenic variants, or imprinting defects. Current methods of differentiating the etiology require molecular testing, which is sometimes difficult to obtain. Recently, computer‐based facial analysis systems have been used to assist in identifying genetic conditions based on facial phenotypes. We sought to understand if the facial‐recognition system DeepGestalt could find differences in phenotype between molecular subtypes of AS. Images and molecular data on 261 individuals with AS ranging from 10 months through 32 years were analyzed by DeepGestalt in a cross‐validation model with receiver operating characteristic (ROC) curves generated. The area under the curve (AUC) of the ROC for each molecular subtype was compared and ranked from least to greatest differentiable phenotype. We determined that DeepGestalt demonstrated a high degree of discrimination between the deletion subtype and UPD or imprinting defects, and a lower degree of discrimination with the UBE3A pathogenic variants subtype. Our findings suggest that DeepGestalt can recognize subclinical differences in phenotype based on etiology and may provide decision support for testing.
Scale‐up of the cross‐flow flat‐plate airlift photobioreactor
Authors: Zhao Luhaibo , Gu Yu , Peng Ci , Tang Zhiyong 
Published: June 2020

AbstractTo commercialize large‐scale and high‐density microalgae cultivation, a 10L cross‐flow flat‐plate airlift photobioreactor (CFA‐PBR) was designed and amplified to two types of 40L CFA‐PBRs via different scaling‐up methods. The multiphase flow characteristics and bioreaction process of these CFA‐PBRs were studied by experiments and computational fluid dynamics (CFD) simulation. The results show that the parameters of CFA‐PBRs, such as bubble size, gas holdup, mass transfer, and photosynthetic reaction efficiency, mainly conform to the principle of geometric similarity under the same light thickness and superficial gas velocity, which is significant for large‐scale industrial applications of the efficient high‐density algae cultivation.
Impact of model selection on predicted contaminant degradation in water treatment
Authors: Gorzalski Alexander S. , Harrington Gregory W. , Coronell Orlando 
Published: June 2020

AbstractModeling contaminant degradation is fundamental to the design, operation, and regulation of reactive contaminants in water treatment reactors. The plug flow reactor (PFR) t10 model has represented residence time distribution (RTD) in pathogen regulation for more than 25 years despite known limitations. Alternative RTD models have been developed, including the tanks‐in‐series (TIS), segregated flow (SF), and reactor network (RN) models. Tracer data were gathered from 35 reactors and their RTDs modeled using the PFR, PFR t10, TIS, SF, and RN models. Predicted contaminant degradation across reactor models was studied, and guidance was developed for selecting RTD models at different log reduction values (LRVs). This study demonstrated that the PFR t10 model may overpredict contaminant degradation at LRVs >2‐log and that the TIS, SF, or RN model should be used for these higher LRVs. A graphical method was also developed for rapidly screening predicted treatment efficacy for a range of contaminants.
A facile synthesis of multifunctional carbon dots as fluorescence ‘turn on’ and ‘turn off’ probes for selective detection of Al3+ and 2,4,6‐trinitrophenol
Authors: Zhao Feifei , Zhang Tianyi , Yang Yu , Lü Changli 
Published: June 2020

AbstractCarbon dots (CDs) have drawn increasing interests due to their unique optical properties and promising application in various fields. In this study, citric acid (CA) and 5‐chloromethyl‐8‐hydroxyquinoline (LQ) were used to synthesize nitrogen‐doped CDs as novel fluorescent probes using a one‐step solvothermal route. The as‐prepared CDs had strong blue‐white fluorescence emission when excited at 405 nm wavelength with a quantum yield (QY) of 25%, behaving with high ion concentration stability. Water‐soluble CDs with a 8‐hydroxyquinoline structure on their surface could be used to detect Al3+ using a ‘turn on’ mechanism and trinitrophenol (TNP) using a ‘turn off’ mechanism with detection limits of 229 nM and 44.4 nM, respectively. Al3+ enhances the fluorescence of CDs by forming a coordination complex to generate a fluorescence synergistic role and limit CD nonradiative transition. TNP quenched the fluorescence with high selectivity and sensitivity, which was attributed to the inner filter effect and static quenching. These results indicated that these CDs with their unique ‘turn on’ and ‘turn off’ nature have potential application in the environmental protection field and in prevention of terrorist threats.
Parkinson's disease or atypical parkinsonism? The importance of acoustic voice analysis in differential diagnosis of speech disorders
Authors: Kowalska‐Taczanowska Renata , Friedman Andrzej , Koziorowski Dariusz 
Published: June 2020

AbstractIntroductionSpeech disorder is a common clinical manifestation in patients with Parkinson's disease and atypical parkinsonian syndromes and tends to occur before the onset of the axial parkinsonian symptoms. Due to parkinsonian features that overlap those of Parkinson's disease, the differentiation of voice and a speech disorder is a challenge for clinicians primarily in the early stage of the disease.MethodsSpeech samples were obtained from 116 subjects including 30 cases of Parkinson's disease, 30 cases of progressive supranuclear palsy, 30 cases of multiple system atrophy, and control group consisted of 26 subjects. Differential diagnosis of dysarthria subtypes was based on the quantitative, acoustic analysis of particular speech components. Additionally, Voice Handicap Index questionnaire was taken into account to differentiate the severity of voice impairment in the study groups.ResultsOur results showed significant differences in the distribution of acoustic parameters between Parkinson's disease and atypical parkinsonian syndromes. A mixed type of dysarthria with a combination of hypokinetic, spastic, and atactic features has been found in patients with atypical parkinsonism. In patients with the clinical diagnosis of the parkinsonian variant of multiple system atrophy, ataxic components of dysarthria were observed. Patients with PD presented pure hypokinetic dysarthria. Some parameters may be used as a marker for the diagnosis of the initial stage of PD. Voice impartment was significantly more frequent and severe in atypical parkinsonism than in Parkinson's disease.ConclusionAcoustic voice analysis is a very sensitive and noninvasive tool, provides objective information for the assessment of different speech components, has the specific potential to provide quantitative data essential for the improvement of the diagnostic process, and maybe a useful instrument in the differential diagnosis of parkinsonian syndromes.
Modulating the function of ABCB1: in vitro and in vivo characterization of sitravatinib, a tyrosine kinase inhibitor
Authors: Yang Yuqi , Ji Ning , Cai Chao‐Yun , Wang Jing‐Quan , Lei Zi‐Ning , Teng Qiu‐Xu , Wu Zhuo‐Xun , Cui Qingbin , Pan Yihang , Chen Zhe‐Sheng 
Published: June 2020

AbstractBackgroundOverexpression of ATP‐binding cassette (ABC) transporter is a major contributor to multidrug resistance (MDR), in which cancer cells acquire resistance to a wide spectrum of chemotherapeutic drugs. In this work, we evaluated the sensitizing effect of sitravatinib, a broad‐spectrum tyrosine kinase inhibitor (TKI), on ATP‐binding cassette subfamily B member 1 (ABCB1)‐ and ATP‐binding cassette subfamily C member 10 (ABCC10)‐mediated MDR.MethodsMTT assay was conducted to examine cytotoxicity and evaluate the sensitizing effect of sitravatinib at non‐toxic concentrations. Tritium‐labeled paclitaxel transportation, Western blotting, immunofluorescence analysis, and ATPase assay were carried out to elucidate the mechanism of sitravatinib‐induced chemosensitization. The in vitro findings were translated into preclinical evaluation with the establishment of xenograft models.ResultsSitravatinib considerably reversed MDR mediated by ABCB1 and partially antagonized ABCC10‐mediated MDR. Our in silico docking simulation analysis indicated that sitravatinib strongly and stably bound to the transmembrane domain of ABCB1 human‐mouse chimeric model. Furthermore, sitravatinib inhibited hydrolysis of ATP and synchronously decreased the efflux function of ABCB1. Thus, sitravatinib could considerably enhance the intracellular concentration of anticancer drugs. Interestingly, no significant alterations of both expression level and localization of ABCB1 were observed. More importantly, sitravatinib could remarkably restore the antitumor activity of vincristine in ABCB1‐mediated xenograft model without observable toxic effect.ConclusionsThe findings in this study suggest that the combination of sitrvatinib and substrate antineoplastic drugs of ABCB1 could attenuate the MDR mediated by the overexpression of ABCB1.
False‐positive semiquantitative immunochromatography assays for procalcitonin in three patients with rheumatoid arthritis—A case series
Authors: Sato Hiroe , Ito Satoshi , Nakazono Kiyoshi , Kurosawa Yoichi , Nozawa Yukiko , Nakatsue Takeshi , Wada Yoko , Kuroda Takeshi , Suzuki Yoshiki , Nakano Masaaki , Narita Ichiei 
Published: June 2020

AbstractWe report three rheumatoid arthritis (RA) patients with false‐positive procalcitonin (PCT) based on semiquantitative immunochromatography assays without infection, but who had negative PCT assay results based on quantitative methods. Immunochromatography was useful for screening; however, other heterophilic antibodies rather than rheumatoid factor were possible to affect, especially in RA flare.
The challenge in diagnosing cardiac tumors to avoid unnecessary cardiac surgery
Authors: Joseph Gowsini , Zelechowski Marek Wojciech , Gronlund Jens , Zaremba Tomas 
Published: June 2020

AbstractCardiac magnetic resonance in cardiac mass: Cardiac magnetic resonance (CMR) should be considered in the evaluation of patients with cardiac mass. Especially, when the diagnosis is not certain, CMR could provide paramount information that could be helpful for the decision on cardiac surgery.
Dental rehabilitation of a cleft lip and palate patient by implant‐supported overdenture: A case report
Authors: Arshad Mahnaz , Ameri Narges , Heidari Abdolreza , Shirani Gholamreza 
Published: June 2020

AbstractOur patient was a 27‐year‐old male with a unilateral cleft lip and palate. Considering the mobility of bone segments around the cleft, an implant‐supported removable overdenture (obturator) was fabricated, which improved the function and esthetics and led to patient satisfaction. The treatment results are still satisfactory after 5 years of follow‐up.
Popular decision rules in SIMCA: Critical review
Authors: Pomerantsev Alexey L. , Rodionova Oxana Ye 
Published: June 2020

AbstractSoft independent modeling of class analogy (SIMCA by Wold) is a statistically based class modeling approach, which has numerous modifications. Two basic statistics, the score and orthogonal distances, are utilized in all known SIMCA versions. The difference between the methods is the way they interpret and apply statistics in decision rules. The four most popular SIMCA decision rules, simple (by Massart), alternative (by Wise), combined index (by Joe Qin), and data driven (by Pomerantsev), are described in detail and compared using simulated and real‐world cases. The examples originate from various fields, and what is more important for statistical analysis is that they present different statistical structures. It is shown that depending on the way the decision rule is built, different SIMCA results can be obtained.
A metagenomic study of biliary microbiome change along the cholecystitis‐carcinoma sequence
Authors: Song Xiaoling , Wang Xu'an , Hu Yunping , Li Huaifeng , Ren Tai , Li Yongsheng , Liu Liguo , Li Lin , Li Xuechuan , Wang Ziyi , Huang Wen , Bao Runfa , Zhang Yijian , Li Maolan , Wang Xuefeng , Liu Feng , Gu Jun , Zheng Linhui , Wu Wenguang , Liu Yingbin 
Published: June 2020

AbstractBackgroundGallbladder cancer (GBC) is the most common cancer type of the biliary tract, and an association has been found between chronic calculous cholecystitis (CCC) and an increased incidence of GBC mortality. An understanding of the relationship between CCC and its carcinogenesis may enable us to prevent and cure GBC. In this study, we attempted to explore changes in the microbiome profile that take place during the transition from chronic cholecystitis mucosa to malignant lesions.ResultsSeven paired human GBC and CCC samples were provided by patients who had undergone laparoscopic cholecystectomy or radical cholecystectomy. Mucosal DNA extraction and metagenomic sequencing were performed to evaluate changes in the microbiota between the two groups. We found that GBC patients and CCC patients shared similar stable and permanent dominant species and showed apparent differences in their biliary microbial composition and gene function. Peptostreptococcus stomatis and Enterococcus faecium may potentially play a role in GBC progression. In addition, the metagenomic species profiles, co‐abundance and co‐exclusion correlations, and CAZyme prevalence showed significant differences between the CCC and GBC groups.ConclusionOur data suggested that changes in the microbiota between CCC and GBC may help deepen our understanding of the complex spectrum of different microbiotas involved in the development of GBC. Although the cohort size was small, this study has presented the first evidence of the existence of an altered biliary microbiota in GBC, which is clearly different from that in CCC patients.
Plasma parameters of microarcs towards minuscule discharge gap
Authors: Baeva Margarita , Loffhagen Detlef , Becker Markus M. , Siewert Erwan , Uhrlandt Dirk 
Published: June 2020

AbstractThis paper describes the behaviour of the plasma parameters of microarcs generated between a cooled copper anode and a ceriated tungsten cathode by means of a one‐dimensional unified non‐equilibrium model for gap lengths between 15 and 200 μm and current densities from 2 × 105 up to 106 A/m2. The results obtained show that the decrease of the gap length to a few tens of micrometres for a given current density results in a progressive shrinking of the quasi‐neutral bulk in the microplasma and its complete disappearance. The decrease of the gap length further leads to an increase of the discharge voltage and the electron temperature and to slightly less heating of the
gas.
Optimal Resource allocation in NOMA‐based M2M communication using Hybrid Rider Optimization with FireFly: Power Saving Strategy of IoT
Authors: K Selvam , K Kumar 
Published: June 2020

SummaryNowadays, the Orthogonal Multiple Access (OMA) principle has utilized for allocating proper radio resources in wireless networks. However, as the count of users rises, OMA‐based approaches may not satisfy the stringent emerging requirements including very low latency, very high spectral efficiency, and massive device connectivity. Moreover, there are significant challenges in cellular‐enabled Machine‐to‐Machine (M2M) communications due to the unique features of M2M‐based applications. In order to overwhelm these challenges, non‐orthogonal multiple access (NOMA) principles emerge as a solution to enhance the spectral efficiency while allowing some degree of multiple access interference at receivers. Hence, this paper intends to develop an optimal resource allocation mechanism for M2M communication. Here, the nonlinear energy harvesting performed with the aid of an accessing technology termed as NOMA. The key objective of the proposed resource allocation model is the minimization of the total energy consumption of the network. For attaining the minimized power consumption, the time allocation, and transmission power of NOMA is optimally tuned by a hybrid optimization algorithm. The proposed hybrid algorithm merges the beneficial concepts of Rider Optimization Algorithm (ROA) and FireFly (FF) algorithm and implements a new algorithm termed as FireFly Modified Bypass‐based Rider Optimization Algorithm (FMB‐ROA). Finally, the analysis of total energy concerning various constraints validates the performance of the proposed model over conventional models.