bluuebunny/crossref_metadata_2025_split huggingface dataset:
SNX10 and PTGDS are Associated with the Progression and Prognosis of Cervical Squamous Cell Carcinoma
Authors: Jiang Pinping , Cao Ying , Gao Feng , Sun Wei , Liu Jinhui , Ma Ziyan , Xie Manxin , Fu Shilong 
Published: October 2020

Abstract
Background: Cervical cancer (CC) is the primary cause of death in women. This study sought to investigate the potential mechanism and prognostic genes of CC. Methods: We downloaded four gene expression profiles from GEO. The RRA method was used to integrate and screen differentially expressed genes (DEGs) between CC and normal samples. Functional analysis was performed by clusterprofiler. We built PPI network by Search Tool for the Retrieval of Interacting Genes Database (STRING) and selected hub modules via Molecular COmplex Detection (MCODE). CMap database was used to find molecules with therapeutic potential for CC. The hub genes were validated in GEO datasets, Gene Expession Profiling Interactive Analysis (GEPIA), immunohistochemistry, Cox regression analysis, TCGA methylation analysis and ONCOMINE were carried out. ROC curve analysis and GSEA were also performed to describe the prognostic significance of hub genes. Results: Functional analysis revealed that 147 DEGs were significantly enriched in binding, cell proliferation, transcriptional activity and cell cycle regulation. PPI network screened 30 hub genes, with CDK1 having the strongest connectivity with CC. Cmap showed that apigenin, thioguanine and trichostatin A might be used to treat CC(P<0.05). Eight genes (APOD, CXCL8, MMP1, MMP3, PLOD2, PTGDS, SNX10 and SPP1) were screened out through GEPIA. Of them, only PTGDS and SNX10 had not appeared in previous studies about CC. The validation in GEO showed that PTGDS showed low expression in tumor tissues while SNX10 showed high expression in tumor tissues. Their expression profiles were consistent with the results in immunohistochemistry. ROC curve analysis indicated that the model had a good diagnostic efficiency(AUC= 0.738). GSEA showed that the two genes were associated with the chemokine signaling pathway(P<0.05). TCGA methylation analysis showed that patients with lowly-expressed and highly-methylated PTGDS had a worse prognosis than those with highly-expressed and lowly-methylated PTGDS (p=0.037). Cox regression analysis showed that SNX10 and PTGDS were independent prognostic indicators for OS among CC patients(P=0.007 and 0.003). Conclusions: PTGDS and SNX10 showed abnormal expression and methylation in CC. Both genes might have high prognostic value of CC patients..
Efficacy and Safety of Divaza for The Correction of Oxidative Disturbances in Patients with Cerebral Atherosclerosis: A Randomized Controlled Trial
Authors: Fateeva Victoria V. , Lashch Nataliia U. , Kamchatnov Pavel R. , Fedorova Tatiana N. , Muzychuk Olga A. , Khacheva Kristina K. , Pizova Nataliya V. , Malygin Alexander U. , Shavlovskaya Olga A. , Nikulina Kseniya V. , Abrosimov Аlbert V. , Gerasimova Yulia A. , Glushkov Konstantin S. , Lebedeva Anna V. 
Published: October 2020

Abstract
Objective: To determine if Divaza was safe and effective for the correction of oxidative disturbances and to stabilize cognitive impairment in patients with cerebral atherosclerosis. Study design: 12-week multicenter, randomized, double-blind, placebo-controlled, prospective trial in parallel groups.Setting: 10 clinical centers across the Russian Federation.Interventions: Patients were randomized into two groups and instructed to take either 2 tablets of the study drug or placebo 3 times per day in conjunction with basic therapy. Outcomes: The primary outcome was a change in the average endogenous antioxidant potential after the completion of the study. The laboratory indicators of oxidative stress were analyzed at baseline and then after 12 weeks of therapy using iron-induced chemiluminescence analysis. The Montreal cognitive assessment test was used as a secondary outcome measure to evaluate cognitive impairment at the end of the study.Results: 124 outpatients with a mean age of 60.7±7.6 years were enrolled and randomly assigned to receive Divaza (n=65) or placebo (n=59). The Administration of Divaza restored the activity of the endogenous antioxidant system. The change in the average level of lipoprotein resistance to oxidation after 12 weeks of therapy, compared to the baseline, was significantly higher in the Divaza group (14.8±14.7 [14.8±14.7] seconds latent period versus 6.4±16.9 [6.9±16.7] seconds in the placebo group (p=0.007 [p=0.0107]). In addition, a recovery of cognitive impairment was observed in all patients of the Divaza group at the end of treatment; this was significantly better when compared with the placebo group (100 [100] % versus 89.5 [89.1] %, respectively, p=0.0272 [p=0.0128]). The treatment was safe, well-tolerated, and had a high compliance rate. Conclusions: Divaza is a safe and effective therapeutic option for attenuating oxidative stress and recovery of cognitive impairment in patients with cerebral atherosclerosis.Trial registration: clinicaltrials.gov NCT03485495, of April 02, 2018.
The Impact of Smoking Status on the Long-Term Prognosis of Male Patients Underwent Percutaneous Coronary Intervention of Left Main Coronary Artery
Authors: QIAO Shu-bin , Hao-bo TENG , Chao GUO , Chang-dong GUAN , Xin-yu WANG , Hao-bo XU , Yu-shi CHUN , Juan WANG , Jian-song YUAN , Wei-xian YANG , Bo XU 
Published: October 2020

Abstract
Objective: To evaluate the impact of smoking status on the long-term prognosis of male patients with left main coronary artery lesions who received percutaneous coronary intervention. Methods: A retrospective analysis was conducted. A total of 3122 male patients with left main lesions who received percutaneous coronary intervention in our hospital were categorized by smoking status at admission: 1207 in the non-smokers group, 1339 in the current smokers group, and 576 in the ex-smokers group. The patients were followed up for 3 years. The main study endpoints were major cardiovascular adverse events (composite endpoints of all-cause death, all myocardial infarctions and revascularization) and target lesion failure (composite endpoints of cardiogenic death, target vessel-related myocardial infarction, target vessel-related blood flow reconstruction). Results: The patients in the current smokers group was younger than the non-smokers group and the ex-smokers group (p<0.0001). In terms of prognosis, no statistical significance observed in the incidence of composite end point of MACE (p=0.9866), target lesion failure (p=0.2522), and stent thrombosis (p=0.2118), all-cause death (p=0.3130), cardiogenic death (p=0.2509), revascularization (p=0.5028), target vessel-related revascularization (p=0.9866), and stroke (p=0.3895), among the three groups. The current smokers group had the lowest incidence of myocardial infarction while ex-smokers group had the highest incidence rate(5.67%vs.5.10%vs.2.97%,p=0.0072), and so is the incidence of myocardial infarction related to target vessels (5.48%vs.5.10%vs.2.89%,p=0.0067). According to Cox regression analysis, history of myocardial infarction(HR=1.339，95%CI：1.042-1.722，p=0.0227), and baseline SYNTAX score(HR=1.0333, 95%CI:1.017-1.049,p<0.0001) are independent risk factors of MACE, while current smoking(HR=0.793,95%CI：0.646-0.974,p=0.0274) proves to be an independent protective factor of target vessel related myocardial infarction. Conclusion: For male patients with the left main coronary artery lesions receiving PCI, smoking has no impact on the long-term MACE or target lesion failure, current smoking is an independent protective factor for target vessel related myocardial infarction.
A service review of the patient focused outcomes of neuroma excision at a mean follow-up of 8 years and 10 months
Authors: Coutts Andrew , Kilmartin Tim 
Published: October 2020

Abstract
Background - While the majority of patients do well following neuroma excision, good outcomes are far from universal. Hence we always provide a course of non-surgical treatment prior to surgery – footwear advice, orthotics, patient education and up to 3 corticosteroid injections. Furthermore, we wanted to understand and quantify the risks and complication to allow us to be able to provide this information to patients during informed consent.Methods - Between 2000 and 2012 neuroma excision from the 2nd intermetatarsal or 3rd intermetatarsal space was carried out consecutively on 42 cases (36 patients), for the treatment of IM neuroma pain. Three patients from the cohort died prior to follow-up and 6 cases failed to attend for review. The outcomes of surgery were measured in a service review using a visual analogue pain scale, the Manchester Oxford Foot Questionnaire, clinical examination of the foot and consultation with the patient where any adverse issues were recorded. The mean follow-up period was 8-years and 10-months (range 61-194 months). At the time of surgery, the mean age of the patients was 54-years of age (range 25-74 years) and at follow-up the mean age was 63-years of age (range 31-85 years).Results - Twenty four cases (73%) reported they were completely satisfied with the results of their surgery, 8 cases (24%) were satisfied with reservations and 1 case (3%) reported they were dissatisfied. All cases agreed they would be happy to undergo surgery under similar circumstance again if required, i.e. under local anaesthesia on a day case basis. 2 cases (6%) reported recurrence of symptoms and required revision surgery which was performed in one case at 9-years and in the other at 11-years. After further excision of nerve tissue, their symptoms resolved.Conclusion - Neuroma excision is a reasonably safe and effective treatment for neuroma pain when non-surgical treatments fail to resolve symptoms. Our approach requires meticulous clinical examination and history taking, up to three cortisone injections and then ultrasound examination before the patient is listed for surgery. In this way we hope to minimise the risk of incorrect diagnosis and continued pain post excision due to the presence of undiagnosed capsulitis.
Evaluation of A Mobile Application Tool (BiliNorm) To Improve Care for Newborns with Hyperbilirubinemia in Indonesia
Authors: Sampurna Mahendra Tri Arif , Ratnasari Kinanti A. , Irawan Zahra S. , Etika Risa - , Utomo Martono Tri , Corebima Brigitta I. R.V. , Sauer Pieter J.J. , Bos Arend F. , Hulzebos Christian V. , Dijk Peter H. 
Published: October 2020

Abstract
Background: Hyperbilirubinemia is more frequently seen in low- and middle-income countries such as Indonesia than in high-income countries. A lack of adherence to existing guidelines for the diagnosis and treatment of hyperbilirubinemia might be one of the factors contributing to this difference. In Indonesia, we developed a new national guideline for hyperbilirubinemia. To assist healthcare workers in the use of this guideline, we developed a web-based app (BiliNorm) to be used on a smartphone. It shows the bilirubin level of the patient on the nomogram and advises which treatment should be started. We evaluated whether this app was helpful and whether it improved care for infants with hyperbilirubinemia.Methods: Health care workers from two teaching hospitals in East Java, Indonesia, were trained in the use of the BiliNorm app. Six months after the introduction of the app, a questionnaire was sent to those who worked with the app asking about their experiences. A chart review of infants with hyperbilirubinemia at these two hospitals was then performed for two time epochs. Hyperbilirubinemia management in the six-month period before the introduction of the app was compared with that in the seven-month period after its introduction.Results: Forty-three participants completed the questionnaire. The majority (72%) of the respondents indicated that the BiliNorm app was well received and easy to use; 84% of respondents indicated that the BiliNorm app was helpful in the decision to start phototherapy. The chart review of 255 infants before and 181 infants after the introduction of the app indicated that significantly more infants received treatment according to the guidelines (38% vs 51%, p=0.006). After the introduction of the app fewer infants received phototherapy, without a bilirubin measurement (14% vs 7%, p=0.024). The percentages of infants that were over- and undertreated were not different (34% vs 32% and 14% vs 10%, respectively)Conclusions: The web-based decision tool BiliNorm seems to be a valuable app. It is easy for healthcare workers to use, and it helps them adhere to guidelines. BiliNorm improves care for infants with hyperbilirubinemia and may help to reduce the incidence of severe hyperbilirubinemia in Indonesia.
Genetic and Causal Relationship Between Coffee Intake and Cardiometabolic Risks: Cross-Phenotype Association And Mendelian Randomization Analysis
Authors: Wang Xinpei , Jia Jinzhu , Huang Tao 
Published: October 2020

Abstract
Background: Many epidemiological studies have shown that there is a significant association between coffee intake and cardiometabolic diseases, which may be due to the common genetic structure or causal relationship. Methods: We used linkage disequilibrium score regression analysis to calculate the genetic correlation between coffee intake and 23 cardiometabolic traits (diseases), and then used cross-phenotype association analysis to identify the shared genetic loci for the trait pairs with significant genetic correlation. Besides, a bi-directional Mendelian Randomization analysis was used to explore the causal relationship between coffee intake and 23 cardiometabolic traits (diseases).Results: Coffee intake has a significant genetic correlation (after Bonferroni correction) with body mass index (BMI) (Rg = 0.3713, P-value = 4.13ⅹ10-64), body fat percentage (BF%) (Rg = 0.2810, P-value = 1.81ⅹ10-13), type 2 diabetes (T2D) (unadjusted for BMI) (Rg = 0.1189, P-value = 8.80ⅹ10-6), heart failure (HF) (Rg = 0.2626, P-value = 6.00ⅹ10-9), atrial fibrillation (AF) (Rg = 0.1007, P-value = 4.30ⅹ10-5). There are 203, 18, 86, 13, 38 independent shared loci between coffee intake and BMI, BF%, T2D, HF, AF, respectively, among which 22, 2, 23, 4,13 loci do not achieve genome-wide significance in single trait GWAS. Coffee intake has significant causal effect on BMI (b = 0.0717, P-value = 2.33ⅹ10-5), T2D (unadjusted for BMI, OR = 1.27, P-value = 1.46ⅹ10-7), and intracerebral haemorrhage (ICH) (all types ICH: OR = 1.86, P-value = 3.37ⅹ10-4; deep ICH: OR = 2.12, P-value = 2.93ⅹ10-4 ). And BMI (b = 0.3694, P-value=3.64ⅹ10-154), BF% (b = 0.5500, P-value = 1.68ⅹ10-4), T2D (adjusted for BMI, b = -0.0252, P-value = 4.83ⅹ10-6) and triglycerides (TG) (b = -0.1209, P-value = 4.56ⅹ10-15) have significant causal effect on coffee intake.Conclusions: Our study identified the shared genetic structure and causal relationship between coffee intake and several cardiometabolic traits (diseases), providing a new insight into the mechanism of coffee intake and cardiometabolic traits (diseases).
Low-Dose IL-34 Has No Effect on Osteoclastogenesis But Promotes Osteogenesis of Hbmscs Partly Via Activation of The PI3K/AKT And ERK Signaling Pathways&nbsp;
Authors: Xu Jianxiang , Bai Jinwu , Zhong Huiming , Kuang Zhihui , Zhou Chengwei , Hu Bin , Ni Licheng , Ying Li , Chen Erman , Zhang Wei , Wu Jiaqi , Xue Deting , pan zhijun 
Published: October 2020

Abstract
Background: Inflammatory microenvironment is significant to the differentiation and function of mesenchymal stem cells(MSCs). It evidentially influences the osteoblastogenesis of MSCs. IL-34, a newly discovered cytokine, playing a key role in metabolism. However, the research on its functional role in the osteogenesis of MSCs was rarely reported. Here, we described the regulatory effects of low-dose IL-34 on both osteoblastogenesis and osteoclastogenesis.Methods: We performed the osteogenic effects of hBMSCs by exogenous and overexpressed IL-34 in vitro, so was the osteoclastogenesis effects of mBMMs by extracellular IL-34. CCK-8 was used to assess the effect of IL-34 on the viability of hBMSCs and mBMMs. ALP staining, ARS and TRAP staining were used to evaluate ALP activity, mineral deposition and osteoclastogenesis, respectively. qRT-PCR and Western blotting analysis were performed to detect the expression of target genes and proteins. ELISA was used to evaluate the concentrations of IL-34. In vivo, a rat tibial osteotomy model and an OVX model were established. Radiographic analysis and histological evaluation were performed to confirm the therapeutic effects of IL-34 in fracture healing and osteoporosis. Statistical differences were evaluated by two-tailed Student’s t-test, one-way ANOVA with Bonferroni’s post hoc test and two-way ANOVA with Bonferroni multiple comparisons post hoc test in the comparison of 2 groups, more than 2 groups and different time points of treated groups, respectively. Results: Promoted osteoblastogenesis of hBMSCs was observed after treated by exogenous or overexpressed IL-34 in vitro, confirmed by increased mineral deposits and ALP activity. Furthermore, exogenous or overexpressed IL-34 enhanced the expression of p-AKT and p-ERK. The specific AKT and ERK signaling pathway inhibitors suppressed the enhancement of osteoblastogenesis induced by IL-34. In a rat tibial osteotomy model, imaging and histological analyses testified the local injection of exogenous IL-34 improved bone healing. However, the additional IL-34 has no influence on both osteoclastogenesis of mBMMs in vitro and osteoporosis of OVX model of rat in vivo. Conclusions: Collectively, our study demonstrate that low-dose IL-34 regulates osteogenesis of hBMSCs partly via the PIK/AKT and ERK signaling pathway and enhances fracture healing, with neither promoting nor preventing osteoclastogenesis in vitro and osteoporosis in vivo.
Estimation of Renal Perfusion Based on Measurement of Rubidium-82 Clearance by PET/CT Scanning in Healthy Subjects
Authors: Langaa Stine Sundgaard , Lauridsen Thomas Guldager , Mose Frank Holden , Fynbo Claire Anne , Theil Jørn , Bech Jesper Nørgaard 
Published: October 2020

Abstract
Background: Changes in renal blood flow (RBF) may play a pathophysiological role in hypertension and kidney disease. However, RBF determination in humans has proven difficult. We aimed to confirm the feasibility of RBF estimation based on positron emission tomography/ computed tomography (PET/CT) and rubidium-82 (82Rb) using the abdominal aorta as input function in a 1-tissue compartment model. Methods: Eighteen healthy subjects underwent two dynamic 82Rb PET/CT scans in two different fields of view (FOV). FOV-A included the left ventricular blood pool (LVBP), the abdominal aorta (AA) and the majority of the kidneys. FOV-B included AA and the kidneys in their entirety. In FOV-A, an input function was derived from LVBP and from AA; in FOV-B from AA. 1-tissue compartmental modeling was performed using tissue time activity curves generated from volumes of interest contouring the kidneys, where the renal clearance of 82Rb is represented by the K1 kinetic parameter. To investigate the correct interpretation of K1, we assumed to first estimate effective renal plasma flow (ERPF) by extrapolating clearance values (ml/min/cm3) to whole kidney values (ml/min) using the estimated total kidney volume. Thereafter, RPF was estimated from ERPF using an assumed extraction fraction (0.89). Lastly, RBF was estimated from RPF using measured haematocrit values. Intra-assay coefficients of variation and inter-observer variation were calculated.Results: For both kidneys, K1 values derived from AA did not differ significantly from values obtained from LVBP, neither were significant differences seen between AA in FOV-A and AA in FOV-B, nor between the right and left kidneys. For both kidneys, the intra-assay coefficients of variation were low (~ 5%) for both input functions. The measured K1 of 2.04 ml/min/cm3 suggests an estimated total renal perfusion normalized to body surface area of 628 ± 95 ml/min/1.73 m2 and subsequently an estimated RBF of 1091 ± 162 ml/min/1.73 m2.
How does the quantum structure of electromagnetic waves describe quantum redshift?
Authors: Kalhor Bahram , Mehrparvar Farzaneh , Kalhor Behnam 
Published: October 2020

Abstract
The paper introduces quantum redshift. By using the quantum structure of the electromagnetic waves, we can describe the redshift. Losing the quanta masses along the traveling in the space is the reason of the decreasing the frequency of the electromagnetic waves. Recursive quantum redshift predict distance of the objects by calculating the z parameter of the waves since they have emitted. Non-recursive quantum redshift is a fast and good approximation of the recursive quantum redshift. The distances in the quantum redshift is less than the distances in the accelerated expansion space theory. The paper provides z parameter of distances between zero and 12 billion light years.
Identification of Aberrantly Methylated and Silenced Genes in Acute Myeloid Leukemia by Combined Methylation/expression Analysis
Authors: Guo Chao , Ju Qian-qian , Zhang Chun-xia , Gong Ming , Gao Ya-yue , Li Zhenling 
Published: October 2020

Abstract
Background Aberrant genomic methylation plays an important role in pathogenic process of acute myeloid leukemia (AML) by silencing tumor suppressor genes (TSG). While the key aberrantly methylated genes and related pathways have not been well understood yet, which we aimed to reveal by combined analysis of methylation and expression datasets. The prognostic significance was validated by survival analysis derived from TCGA database. Methods Micro-array data of GSE 15061 and GSE58477 were downloaded from Gene Expression Omnibus (GEO) database. The differentially methylated regions (DMR) and differentially expressed genes (DEG) were identified using R program (R 3.6.1). Over-representation analysis was performed to obtain the enriched biological processes and pathways. Cox hazards analysis was employed to select the genes significantly associated with AML survival, using the data derived from the Cancer Genome Atlas (TCGA) database. Subgroup analysis, regarding induction type, was conducted to identify biomarkers for HMA treatment. Furthermore, SYNJ2 associated genome-wide gene/miRNA expression and methylation profile were explored. Results A total of 198 aberrant methylation related underexpressed genes were identified. Univariable analysis revealed methylation level of 6 out of 198 genes (CORO1A/MPO/SYNJ2/EHD1/GAS2L1/SLC11A1) were significantly associated with AML survival. SYNJ2 methylation was an independent predictor for OS. Notably, subgroup analysis revealed hypermethylation of CORO1A predicted better OS in HMA group. Further gene set enrichment analysis indicated SYNJ2-associated activation of PI3K-Akt/NF-kappaB/JAK-STAT signaling and checkpoint pathway. The microRNAs, such as miR217/miR485-3p/miR-889-3p, were downregulated in SYNJ2-hypermethylated group, leading to potential HOXA13 upregulation. Conclusion The prognostic methylation signature was revealed in our studies, and SYNJ2 was proved as an independent prognostic factor. Methylation of CORO1A may serve as biomarker for HMA treatment in AML.