bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physical activity and weight following car ownership in Beijing, China: quasi-experimental cross sectional study
Authors: Anderson Michael L , Lu Fangwen , Yang Jun 
Published: January 2020

AbstractObjectiveTo determine the implications of car ownership for physical activity and weight in a global city.DesignQuasi-experimental cross sectional study.SettingBeijing, China, 2011-15.ParticipantsPeople aged 18 and older from a random sample of households who had entered a permit lottery to purchase a vehicle between January 2011 and November 2015.InterventionsPermit allowing purchase of a vehicle within six months of permit issuance.Main outcome measuresTransit use (number of subway and bus rides each week), physical activity (minutes of walking or bicycling each day), and weight, measured once in early 2016.ResultsOf 937 people analysed in total, 180 had won a permit to purchase a new vehicle. Winning the permit lottery resulted in the purchase of an additional vehicle 91% of the time (95% confidence interval 89% to 94%; P<0.001). About five years after winning, winners took significantly fewer weekly transit rides (−2.9 rides (−5.1 to −0.7); P=0.01) and walked and cycled significantly less (−24.2 minutes (−40.3 to −8.1); P=0.003) than those who did not win the lottery. Average weight did not change significantly between lottery winners and losers. Among those aged 50 and older, however, winners’ weight had increased relative to that of losers (10.3 kg (0.5 to 20.2); P=0.04) 5.1 years after winning.ConclusionsThese data indicate that vehicle ownership in a rapidly growing global city led to long term reductions in physical activity and increase in weight. Continuing increases in car use and ownership in developing and middle income countries could adversely affect physical health and obesity rates.
Gender differences in how scientists present the importance of their research: observational study
Authors: Lerchenmueller Marc J , Sorenson Olav , Jena Anupam B 
Published: January 2020

AbstractObjectivesWomen remain underrepresented on faculties of medicine and the life sciences more broadly. Whether gender differences in self presentation of clinical research exist and may contribute to this gender gap has been challenging to explore empirically. The objective of this study was to analyze whether men and women differ in how positively they frame their research findings and to analyze whether the positive framing of research is associated with higher downstream citations.DesignRetrospective observational study.Data sourcesTitles and abstracts from 101 720 clinical research articles and approximately 6.2 million general life science articles indexed in PubMed and published between 2002 and 2017.Main outcome measuresAnalysis of article titles and abstracts to determine whether men and women differ in how positively they present their research through use of terms such as “novel” or “excellent.” For a set of 25 positive terms, we estimated the relative probability of positive framing as a function of the gender composition of the first and last authors, adjusting for scientific journal, year of publication, journal impact, and scientific field.ResultsArticles in which both the first and last author were women used at least one of the 25 positive terms in 10.9% of titles or abstracts versus 12.2% for articles involving a male first or last author, corresponding to a 12.3% relative difference (95% CI 5.7% to 18.9%). Gender differences in positive presentation were greatest in high impact clinical journals (impact factor >10), in which women were 21.4% less likely to present research positively. Across all clinical journals, positive presentation was associated with 9.4% (6.6% to 12.2%) higher subsequent citations, and in high impact clinical journals 13.0% (9.5% to 16.5%) higher citations. Results were similar when broadened to general life science articles published in journals indexed by PubMed, suggesting that gender differences in positive word use generalize to broader samples.ConclusionsClinical articles involving a male first or last author were more likely to present research findings positively in titles and abstracts compared with articles in which both the first and last author were women, particularly in the highest impact journals. Positive presentation of research findings was associated with higher downstream citations.
Prevalence and trends in dietary supplement use among US adults with diabetes: the National Health and Nutrition Examination Surveys, 1999–2014
Authors: Li Jing , Li Xinli , Gathirua-Mwangi Wambui , Song Yiqing 
Published: January 2020

ObjectiveTo evaluate overall prevalence and trends of use of any supplements, multivitamins/multiminerals (MVMM), individual vitamins, minerals, and non-vitamin, non-mineral (NVNM) supplements among adults with diabetes in the USA.Research design and methodsWe used a nationally representative sample from the National Health and Nutrition Examination Survey collected between 1999 and 2014. Information on supplement use in the preceding 30 days was collected during interview over 8 continuous 2-year waves. To account for the complex sampling design, weighted analyses were conducted among 6688 US adults with diabetes aged 20–85 years and also stratified by age, sex, race/ethnicity, education, comorbidity status, and diabetes duration.ResultsOverall, the prevalence of any supplement use (52%–58%;Pfor trend=0.08) and that of any mineral use (47%–51%;Pfor trend=0.23) seemed stable over the years studied. Reported use of MVMM slightly decreased from 36% to 32% (Pfor trend=0.006). Use of any vitamin products significantly increased from 47% to 52% (Pfor trend=0.03). Use of some individual supplements, especially vitamin D, choline, lycopene, and fish oil supplements, significantly increased, while some vitamins, minerals and NVNM supplements decreased over the years. In addition, the trend of any supplement use varied by age, sex, race/ethnicity, or education, but not by diabetes duration or diabetic comorbidities.ConclusionsAmong US patients with diabetes, use of any dietary supplements or any minerals remained stable, while MVMM use slightly decreased and use of any vitamins increased. Additionally, use of several individual supplements varied significantly over the 16-year period studied.
Chronic medical conditions and their association with crash risk and changes in driving habits: a prospective study of the GAZEL cohort
Authors: Naredo Turrado Juan , Orriols Ludivine , Contrand Benjamin , Zins Marie , Salmi Louis-Rachid , Lafont Sylviane , Lagarde Emmanuel 
Published: January 2020

ObjectivesTo assess crash risk and driving habits associated with chronic medical conditions among drivers entering old age.DesignProspective cohort study.SettingFrench cohort GAZEL.Participants12 460 drivers in the analysis of road traffic crash, among whom 11 670 completed the follow-up period (2007–2014). We assessed driving cessation among 11 633 participants over the same period, and mileage and driving avoidance among the 4973 participants who returned a road safety questionnaire in 2015.Main outcome measuresYearly occurrence of at least one road crash as a driver; time to driving cessation; mileage; driving avoidance: at night, with bad weather, in heavy traffic, with glare conditions, over long distances.ResultsSeveral potentially risky conditions (angina, myocardial infarction, coronary disease; stroke;nephritic colic, urinary stones; glaucoma) were associated with lower mileage and/or driving avoidance and did not increase crash risk. Neither driving avoidance nor lower mileage was found for other conditions associated with an increased crash risk: hearing difficulties (adjusted OR 1.19, 95% CI 1.06 to 1.34); joint disorders (1.17, 95% CI 1.06 to 1.30).Depression, anxiety and stresswas associated with an increased crash risk (1.23, 95% CI 1.01 to 1.49) despite increased driving avoidance. Parkinson’s disease was associated with driving cessation (adjusted HR 32.61, 95% CI 14.21 to 65.17).ConclusionsDepending on their condition, and probably on the associated risk perception, drivers entering old age report diverse driving habits. For example, hearing difficulties is a frequent condition, rarely considered a threat to road safety, and nonetheless associated with an increased crash risk.
Sex, blinking, and dry eye
Authors: Culoso Ashley , Lowe Cynthia , Evinger Craig 
Published: January 2020

Blinking sustains the corneal tear film generated by sexually dimorphic lacrimal and meibomian glands. Our study examines whether trigeminal control of blinking is also sexually dimorphic by investigating trigeminal reflex blinking, associative blink modification, and spontaneous blinking in male and female rats before and after unilateral dry eye caused by exorbital gland removal. Before gland removal, female rats exhibited a lower threshold for evoking trigeminal reflex blinks, a weaker effect of associative blink modification, and longer-duration spontaneous blinks than males. Spontaneous blink rate, reflex blink excitability, and occurrence of blink oscillations did not differ between the sexes. Reanalysis of previous data showed that humans showed the same blink sexual dimorphisms as rats. During the first 2 wk of dry eye, trigeminal blink circuit excitability and blink oscillations steadily rose in male rats, whereas excitability and blink oscillations did not change in females. Following dry eye, spontaneous blink duration increased for both males and females, whereas spontaneous blink rate remained constant for males but decreased for females. The associative modification treatment to depress trigeminal blink amplitude initially produced blink depression in males that converted to blink potentiation as trigeminal excitability rose, whereas females exhibited progressively more blink depression. These data indicated that dry eye increased excitability in male trigeminal reflex blink circuits at the expense of circuit modifiability, whereas trigeminal modifiability increased in females. This increased modifiability of female trigeminal blink circuits with dry eye may contribute to the preponderance of females developing the focal dystonia, benign essential blepharospasm. NEW & NOTEWORTHY All the elements controlling the corneal tear film are sexually dimorphic. Blinking, which smooths and maintains the tear film, also exhibits sex differences. Dry eye increases the sexual dimorphisms of blinking, including increased exaggeration of excitability in males and enhanced modifiability of the female trigeminal complex. This increased modifiability may explain female predominance in the development of the focal dystonia, benign essential blepharospasm.
IGF2 Autocrine-Mediated IGF1R Activation Is a Clinically Relevant Mechanism of Osimertinib Resistance in Lung Cancer
Authors: Manabe Tadashi , Yasuda Hiroyuki , Terai Hideki , Kagiwada Harumi , Hamamoto Junko , Ebisudani Toshiki , Kobayashi Keigo , Masuzawa Keita , Ikemura Shinnosuke , Kawada Ichiro , Hayashi Yuichiro , Fukui Kazuhiko , Horimoto Katsuhisa , Fukunaga Koichi , Soejima Kenzo 
Published: January 2020

Abstract

EGFR-mutated lung cancer accounts for a significant proportion of lung cancer cases worldwide. For these cases, osimertinib, a third-generation EGFR tyrosine kinase inhibitor, is extensively used as a first-line or second-line treatment. However, lung cancer cells acquire resistance to osimertinib in 1 to 2 years. Thus, a thorough clarification of resistance mechanisms to osimertinib is highly anticipated. Recent next-generation sequencing (NGS) of lung cancer samples identified several genetically defined resistance mechanisms to osimertinib, such as EGFR C797S or MET amplification. However, nongenetically defined mechanisms are not well evaluated. For a thorough clarification of osimertinib resistance, both genetic and nongenetic mechanisms are essential. By using our comprehensive protein phosphorylation array, we detected IGF1R bypass pathway activation after EGFR abolishment. Both of our established lung cancer cells and patient-derived lung cancer cells demonstrated IGF2 autocrine-mediated IGF1R pathway activation as a mechanism of osimertinib resistance. Notably, this resistance mechanism was not detected by a previously performed NGS, highlighting the essential roles of living cancer cells for a thorough clarification of resistance mechanisms. Interestingly, the immunohistochemical analysis confirmed the increased IGF2 expression in lung cancer patients who were treated with osimertinib and met the established clinical definition of acquired resistance. The findings highlight the crucial roles of cell-autonomous ligand expression in osimertinib resistance. Here, we report for the first time the IGF2 autocrine-mediated IGF1R activation as a nongenetic mechanism of osimertinib resistance in lung cancer at a clinically relevant level.

Implications:
Using comprehensive protein phosphorylation array and patient-derived lung cancer cells, we found that IGF2 autocrine-mediated IGF1R pathway activation is a clinically relevant and common mechanism of acquired resistance to osimertinib.
<p><strong>Lectotypification of the names <em>Plantago weldenii </em>and <em>P. commutata </em>(Plantaginaceae)</strong></p>
Authors: LAZZARO LORENZO , MUGNAI MICHELE , FERRETTI GIULIO , LAZZERI VALERIO , BRÄUCHLER CHRISTIAN , FOGGI BRUNO 
Published: January 2020

Genus Plantago (Plantaginaceae) hosts more than 200 species with complicated nomenclature and taxonomy. Within section Coronopus the two taxa Plantago weldenii and P. commutata have either been treated as different taxa or merged together. As a basis for a thorough taxonomic revision of these taxa, we designate here lectotypes for the two names P. weldenii and P. commutata, also discussing the main typification issues.
Making the Output of Seasonal Climate Models More Palatable to Agriculture: A Copula-Based Postprocessing Method
Authors: Li Ming , Jin Huidong , Brown Jaclyn N. 
Published: January 2020

AbstractSeasonal climate forecasts from raw climate models at coarse grids are often biased and statistically unreliable for credible crop prediction at the farm scale. We develop a copula-based postprocessing (CPP) method to overcome this mismatch problem. The CPP forecasts are ensemble based and are generated from the predictive distribution conditioned on raw climate forecasts. CPP performs univariate postprocessing procedures at each station, lead time, and variable separately and then applies the Schaake shuffle to reorder ensemble sequence for a more realistic spatial, temporal, and cross-variable dependence structure. The use of copulas makes CPP free of strong distributional assumptions and flexible enough to describe complex dependence structures. In a case study, we apply CPP to postprocess rainfall, minimum temperature, maximum temperature, and radiation forecasts at a monthly level from the Australian Community Climate and Earth-System Simulator Seasonal model (ACCESS-S) to three representative stations in Australia. We evaluate forecast skill at lead times of 0–5 months on a cross-validation theme in the context of both univariate and multivariate forecast verification. When compared with forecasts that use climatological values as the predictor, the CPP forecast has positive skills, although the skills diminish with increasing lead times and finally become comparable at long lead times. When compared with the bias-corrected forecasts and the quantile-mapped forecasts, the CPP forecast is the overall best, with the smallest bias and greatest univariate forecast skill. As a result of the skill gain from univariate forecasts and the effect of the Schaake shuffle, CPP leads to the most skillful multivariate forecast as well. Further results investigate whether using ensemble mean or additional predictors can enhance forecast skill for CPP.
The impact of a patient-directed activity program on functional outcomes and activity participation after stroke during inpatient rehabilitation—a randomized controlled trial
Authors: Swank Chad , Trammell Molly , Callender Librada , Bennett Monica , Patterson Kara , Gillespie Jaime , Kapoor Priyanka , Driver Simon 
Published: January 2020

Objective: Individuals post stroke are inactive, even during rehabilitation, contributing to ongoing disability and risk of secondary health conditions. Our aims were to (1) conduct a randomized controlled trial to examine the efficacy of a “Patient-Directed Activity Program” on functional outcomes in people post stroke during inpatient rehabilitation and (2) examine differences three months post inpatient rehabilitation discharge. Design: Randomized control trial. Setting: Inpatient rehabilitation facility. Subjects: Patients admitted to inpatient rehabilitation post stroke. Interventions: Patient-Directed Activity Program (PDAP) or control (usual care only). Both groups underwent control (three hours of therapy/day), while PDAP participants were prescribed two additional 30-minute activity sessions/day. Main measures: Outcomes (Stroke Rehabilitation Assessment of Movement Measure, Functional Independence Measure, balance, physical activity, Stroke Impact Scale) were collected at admission and discharge from inpatient rehabilitation and three-month follow-up. Results: Seventy-three patients (PDAP ( n = 37); control ( n = 36)) were included in the primary analysis. Patients in PDAP completed a total of 23.1 ± 16.5 sessions (10.7 ± 8.5 upper extremity; 12.4 ± 8.6 lower extremity) during inpatient rehabilitation. No differences were observed between groups at discharge in functional measures. PDAP completed significantly more steps/day (PDAP = 657.70 ± 655.82, control = 396.17 ± 419.65; P = 0.022). The Stroke Impact Scale showed significantly better memory and thinking (PDAP = 86.2 ± 11.4, control = 80.8 ± 16.7; P = 0.049), communication (PDAP = 93.6 ± 8.3, control = 89.6 ± 12.4; P = 0.042), mobility (PDAP = 62.2 ± 22.5, control = 53.8 ± 21.8; P = 0.038), and overall recovery from stroke (PDAP = 62.1 ± 19.1, control = 52.2 ± 18.7; P = 0.038) for PDAP compared to control. At three months post discharge, PDAP ( n = 11) completed significantly greater physical activity ( P = 0.014; 3586.5 ± 3468.5 steps/day) compared to control ( n = 10; 1760.9 ± 2346.3 steps/day). Conclusion: Functional outcome improvement was comparable between groups; however, PDAP participants completed more steps and perceived greater recovery.
Treatment of renal calculi without hydronephrosis using a standard channel assisted by a visual puncture system
Authors: Wang Ji-liang , Cui Zhen-yu , Zhu Shi-fan , Yang Wen-zeng , Zhou Hong-yue , Zhang Shi-qing , Fu Ze-sheng , Sun Yun-fei 
Published: January 2020

Objective To study the safety and efficacy of standard channel establishment using a visual puncture system in the treatment of renal calculi without hydronephrosis. Methods We retrospectively analyzed data from 46 patients undergoing percutaneous nephrolithotomy via a standard channel assisted by a visual puncture system, including number of punctures, duration of procedure, hemoglobin level after surgery, calculi clearance rate, and postoperative complications. Results Sixty-nine channels were established, including a single channel in 26 cases, double channel in 17 cases, and three channels in 3 cases. The mean number of punctures was 1.50, mean time to establish the standard channel was 2.6 minutes, and duration of surgery was 31.3 minutes. No significant change in hemoglobin was observed after surgery. Forty-three patients had no residual calculi and three had small residual calculi on the day after surgery, representing a first-phase clearance rate of 93.48% (43/46). No significant complications were observed. Conclusions Hemorrhage during operation can be significantly reduced by establishing a standard channel using a visual puncture system. Moreover, the number of standard channels can be reduced and the incidence of complications lowered using this approach, which should be adopted widely for the treatment of renal calculi without hydronephrosis.
Overexpression of cell-cycle related and expression-elevated protein in tumor (CREPT) in malignant cervical cancer
Authors: Wen Na , Bian Lihua , Gong Jing , Meng Yuanguang 
Published: January 2020

Objective To explore the expression of cell-cycle related and expression-elevated protein in tumor (CREPT), cyclin D1, and transcription factor 4 (TCF4) in patients with cervical carcinoma. Methods This study enrolled 20 patients with cervical cancer and 10 control patients diagnosed with benign cervical lesions undergoing resection at the People’s Liberation Army General Hospital from January 2016 to December 2017. Cervical tissues were collected from all patients and their clinical characteristics were recorded. Protein and mRNA levels of CREPT, cyclin D1, and TCF4 were measured in tissue samples by immunohistochemistry, western blotting, and real-time polymerase chain reaction (PCR) and compared between the two groups. Result Protein and mRNA expression levels of CREPT, cyclin D1, and TCF4 were all significantly higher in the cervical cancer compared with the control group, according to western blot and PCR, respectively. CREPT expression was also significantly higher in the cervical cancer group according to immunohistochemistry. Conclusion Levels of CREPT, cyclin D1, and TCF4 were significantly elevated in cervical carcinoma tissues, and their expression levels were positively correlated, suggesting that these factors might play important roles during the development of cervical carcinoma.
Identification of key genes involved in the pathogenesis of cutaneous melanoma using bioinformatics analysis
Authors: Chen Jianqin , Sun Wen , Mo Nian , Chen Xiangjun , Yang Lihong , Tu Shaozhong , Zhang Siwen , Liu Jing 
Published: January 2020

Objective Malignant melanoma is a highly invasive cancer whose pathogenesis remains unclear. We analyzed the microarray dataset GDS1375 in the Gene Expression Omnibus database to search for key genes involved in the occurrence and development of melanoma. Methods The dataset included 52 samples (7 normal skin and 45 melanoma samples). We identified differentially expressed genes (DEGs) between the two groups and used integrated discovery databases for Gene Ontology (GO) and Kyoto Gene and Genome Encyclopedia (KEGG) pathway analyses. In addition, we used the STRING and MCODE plugins of Cytoscape to visualize the protein-protein interactions (PPI) for these DEGs. Results A total of 509 upregulated and 618 downregulated DEGs were identified, which were enriched in GO terms including integrin binding, protein binding, and structural constituent of cytoskeleton, and in KEGG pathways such as melanogenesis, prostate cancer, focal adhesion, and renin secretion. Three major modules from the PPI networks and 10 hub genes were identified, including CDC20, GNB2, PPP2R1A, AURKB, POLR2E, and AGTR1. Overall survival was low when these six hub genes were highly expressed. Conclusion This bioinformatics analysis identified hub genes that may promote the development of melanoma and represent potential new biomarkers for diagnosis and treatment of melanoma.