bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic analysis of the S. cerevisiae response to the anticancer ruthenium complex KP1019
Authors: Stultz Laura K , Hunsucker Alexandra , Middleton Sydney , Grovenstein Evan , O’Leary Jacob , Blatt Eliot , Miller Mary , Mobley James , Hanson Pamela K 
Published: April 2020

Abstract
Like platinum-based chemotherapeutics, the anticancer ruthenium complex indazolium trans-[tetrachlorobis(1H-indazole)ruthenate(iii)], or KP1019, damages DNA, induces apoptosis, and causes tumor regression in animal models. Unlike platinum-based drugs, KP1019 showed no dose-limiting toxicity in a phase I clinical trial. Despite these advances, the mechanism(s) and target(s) of KP1019 remain unclear. For example, the drug may damage DNA directly or by causing oxidative stress. Likewise, KP1019 binds cytosolic proteins, suggesting DNA is not the sole target. Here we use the budding yeast Saccharomyces cerevisiae as a model in a proteomic study of the cellular response to KP1019. Mapping protein level changes onto metabolic pathways revealed patterns consistent with elevated synthesis and/or cycling of the antioxidant glutathione, suggesting KP1019 induces oxidative stress. This result was supported by increased fluorescence of the redox-sensitive dye DCFH-DA and increased KP1019 sensitivity of yeast lacking Yap1, a master regulator of the oxidative stress response. In addition to oxidative and DNA stress, bioinformatic analysis revealed drug-dependent increases in proteins involved ribosome biogenesis, translation, and protein (re)folding. Consistent with proteotoxic effects, KP1019 increased expression of a heat-shock element (HSE) lacZ reporter. KP1019 pre-treatment also sensitized yeast to oxaliplatin, paralleling prior research showing that cancer cell lines with elevated levels of translation machinery are hypersensitive to oxaliplatin. Combined, these data suggest that one of KP1019’s many targets may be protein metabolism, which opens up intriguing possibilities for combination therapy.
Medizinsystem Homöopathie
Authors: Bleul Gerhard 
Published: April 2020

ZusammenfassungDie Geschichte der Homöopathie war von Beginn an von Kontroversen geprägt. Immer wieder wurden ihre Lehren und ihre Vertreter angegriffen, aber immer wieder gab es auch große Fortschritte in der Entwicklung und der Akzeptanz. Die größten Unterstützer hat die Homöopathie immer unter den Patienten, denen sie geholfen hat und die durch eigene Erfahrung von ihrer Wirkung überzeugt sind. Die Methode ist integraler Bestandteil der gesamten Medizin, weltweit verbreitet und in ihrer Anwendung sehr erfolgreich. Der Beitrag beschreibt die historischen Grundlagen der Homöopathie anhand Hahnemanns „Organon der Heilkunst“, und Fallbeispiele aus der Praxis zeigen ihr breites Anwendungsspektrum.
Untargeted Metabolomics Profiling and Global Semi-quantitation of a
Prescription Chinese Herbal Medicine Formula Yinqiaosan Using UPLC-QTOF-MS with
a Single Exogenous Reference Internal Standard
Authors: Shu Yachun , Mannem Chandana , Xu Gang , Gorityala Shashank , Liu Xiao , Xu Yan 
Published: April 2020

Abstract
Yinqiaosan is a classic Chinese herbal medicine formula that has been
used to treat various bacterial and viral infections by Chinese medicine doctors
for over two centuries. In this work, we developed a comprehensive qualitative
and quantitative method for identification, quantitation, and quality assessment
of chemical constituents of Yinqiaosan formula in four different preparation
forms (i.e., decoction, granule, pill, and tablet), which employed
ultra-performance liquid chromatography quadrupole time-of-flight mass
spectrometry with a single exogenous reference internal standard for untargeted
metabolomics profiling and global semiquantitative analysis. The use of a single
exogenous reference internal standard permitted not only qualitative and
quantitative analyses of multiple herbal components in a single instrument run,
but also cross-comparison of chemical contents in between all four Yinqiaosan
preparation forms. The acquired mass chromatograms were analyzed, quantitated,
and compared using multivariate data analysis for similarities and differences
of chemical constituents in four Yinqiaosan preparation forms. For the first
time, we were able to identify over 100 chemical constituents from each
preparation form using the available database. Among the 49 commonly identified
compounds in the 4 Yinqiaosan preparation forms, 16 have been reported to have
pharmacological activities, which may be used in a network pharmacology study of
Yinqiaosan for exploring the underlying mechanism of the herbal formula.
Ayurveda – alte Heilkunde und modernes Medizinsystem
Authors: Chopra Ananda Samir 
Published: April 2020

ZusammenfassungAyurveda ist eine umfassende wissenschaftliche Heilkunde und Gesundheitslehre, die auf eine rund 2000-jährige Tradition zurückblickt. In Indien ist Ayurveda ein öffentlich anerkanntes Medizinsystem, international wird er meist als komplementäre Medizin praktiziert und erforscht. In diesem Beitrag werden einige grundlegende Aspekte der ayurvedischen Wissenschaft vor allem in Bezug auf Diagnose und Therapie vorgestellt, und eine Kasuistik illustriert beispielhaft die klinische Anwendung von Ayurveda in Deutschland.
Oseltamivir and S-Adenosyl-L-Methionine Combination as Effective
Therapeutic Strategy for Suppression of Oxidative Damage in Lung Caused by
Influenza Virus Infection in Mice
Authors: Mileva Milka , Dimitrova Adriana , Krastev Dimo , Alexandrova Albena , Tsvetanova Elina , Georgieva Almira , Galabov Angel 
Published: April 2020

Abstract
Background and objectives The pathogenesis of influenza infection is
associated with two general processes in the body: (a) lung damage based on
virus replication; (b) overproduction of free radicals, antioxidant
deficiency, and development of oxidative stress. To attack these aspects of
flu pathogenesis, we explored the combined effect of the antiviral agent
oseltamivir, and s-adenosyl-l-methionine (SAM) as a precursor of the
endogenous antioxidant glutathione, in mice infected with influenza
virus.
Methods After inoculation of albino mice with 10 MLD50 of influenza
virus A/Aichi/2/68 (H3N2), oseltamivir was applied
twice a day, for five days post-infection in doses of 1.25 and 2.5
mg/kg. SAM was administered once a day for 10 days, starting 5 days
before infection in doses of 50, 100 and 150 mg/kg.
Results Monotherapy with SAM did not influence the markers of
oxidative stress in the lung. Combination of SAM 50 mg/kg and
oseltamivir 2.5 mg/kg affected best the virological parameters -
viral titer, protection index, and mean survival time, as well as the
biochemical markers of oxidative stress.
Interpretation and conclusions Combining of SAM and oseltamivir in a
dose of 1/4 of optimal therapeutic could be considered as a
perspective therapy of influenza viral infection.
Utility of laser-cut covered self-expandable metal stents for unresectable malignant distal biliary obstruction: a single-center experience
Authors: Marui Saiko , Uza Norimitsu , Yamazaki Hajime , Ota Sakiko , Nakamura Takeharu , Yoshida Hiroyuki , Okada Hirokazu , Hirano Tomonori , Kuwada Takeshi , Sogabe Yuko , Tomono Teruko , Matsumori Tomoaki , Morita Toshihiro , Nishikawa Yoshihiro , Tsuda Motoyuki , Maruno Takahisa , Shiokawa Masahiro , Kodama Yuzo , Seno Hiroshi 
Published: April 2020

Abstract
Background Few reports have evaluated the effectiveness of laser-cut, covered, self-expandable metal stents (LC-CSEMS) for unresectable malignant distal biliary obstruction (MDBO) and whether reintervention is feasible after placement. We describe our experience with LC-CSEMS placement for unresectable MDBO.
Methods Patients undergoing LC-CSEMS placement for unresectable MDBO from November 2014 to December 2018 were reviewed. Recurrent biliary obstruction (RBO), median time to RBO (TRBO), and reintervention were analyzed.
Results 52 patients who underwent LC-CSEMS placement for unresectable MDBO were included in the analysis. The RBO rate was 15 % and the median TRBO was 445 days. Reintervention was attempted in nine patients and stent removal was successful in eight patients.
Conclusions Our experience suggests the effectiveness of LC-CSEMS in patients with unresectable MDBO in terms of stent patency and feasibility of reintervention.
Outcome after Tibial Plateau Levelling Osteotomy and Modified Maquet Procedure in Dogs with Cranial Cruciate Ligament Rupture
Authors: Knebel Julia , Eberle Daniela , Steigmeier-Raith Stephanie , Reese Sven , Meyer-Lindenberg Andrea 
Published: April 2020

Abstract
Objective The aim of this study was to compare outcomes after tibial plateau levelling osteotomy (TPLO) and modified Maquet procedure (MMP) for the treatment of cranial cruciate ligament rupture (CCLR) in dogs using clinical and radiographic evaluation and treadmill-based force plate gait analysis.
Study Design This study was a prospective, randomized, controlled study.
Materials and Methods Sixty-one dogs (76 joints) with CCLR were treated with TPLO (n = 30 dogs, 41 joints) or MMP (n = 31 dogs, 35 joints) and compared with a control group of 16 healthy Labrador Retrievers. Outcomes after surgery were compared by clinical orthopaedic assessment, radiographic evaluation and force plate gait analysis performed preoperatively, and then at 6 weeks, 3 and 6 months postoperatively. For objective comparison of ground reaction forces, the data were compared with the control group. Major complications were reported.
Results A significant improvement in ground reaction forces was reached in all surgically treated dogs. No significant difference was found between the surgical methods at any postoperative re-examination. With regard to peak vertical force (PVF), there were significantly more patients with TPLO within the reference range of healthy dogs at the 3 months re-examination than dogs with MMP. There was no significant difference in mean value comparisons between TPLO and control groups 6 months postoperatively. Compared with the control group, mean values of 93.9% (PVF) and 85.9% (vertical impulse [VI]) were reached by the TPLO group and 89.4% (PVF) and 79.9% (VI) by the MMP group, 6 months postoperatively.No significant differences were found regarding major complications or progression of osteoarthritis.
Conclusions Although no significant differences were found between the surgical methods, TPLO patients showed superiority with regard to clinical outcome.
Neural Recovery Function of the Auditory Nerve in Cochlear Implant Surgery: Comparison between Prelingual and Postlingual Patients
Authors: Carvalho Bettina , Wiemes Gislaine Richter Minhoto , Patrial Netto Luiz , Hamerschmidt Rogerio 
Published: April 2020

Abstract
Introduction Cochlear implants (CIs) enable objective measures of the neural function in implanted patients through the measurements of the neural response telemetry (NRT) and of the Auditory nerve Recovery Function (REC). These measurements help in programming the speech processor and understanding the auditory system.
Objective To compare the NRT and the REC in prelingual and postlingual implanted patients.
Methods An observational, descriptive and prospective study was carried out. The NRT and the REC (through the T0, A, and tau parameters) were evaluated in individuals submitted to CI surgery, who were divided into two groups: prelingual and postlingual patients.
Results In total, 46 patients were evaluated. Data analysis showed no statistically significant difference between the NRT measurements and the T0, A, and Tau of the REC in the comparison between the two groups, except for the NRT in the basal cochlear region.
Conclusion There was no statistically significant difference in the REC in pre- and postlingual patients.
Effect of Fasting On Cognitive Functioning In Healthy Young Adults
Authors: S. Shrunga M. , Shamooda Ayisha , Shaniba Fathimath 
Published: April 2020

Abstract
Background and objective: Cognitive flexibility refers to the ability of the brain to switch from thinking about one concept to another simultaneously. The study was taken up to evaluate the effect of fasting on Cognitive Functioning In Healthy Young Adults.
Methodology: A total of 20 healthy young adults participated in the study. Paper Stroop Color Test was employed to assess inhibition and cognitive flexibility. Participants were given 4 cards (word card color card and color-word card) and were instructed to read the card as told by the clinician. The total duration taken to complete each task and incorrect responses for each task was noted. The same experiment was done in fasting and non-fasting condition for each participant.
Results: The results revealed no statistical significance between the 3 tasks between 2 conditions. Though our present study did not reveal statistical significance difference between 3 tasks and 2 conditions, it was found that the performance was better during the second condition i.e. after fasting condition in comparison to first condition i.e during fasting condition.
Conclusion: The study justifies these findings to be reflected due to fluid deprivation, change in life style and sleep wake up cycles with in turn effects cognitive functioning. The study also highlights on the effectiveness of stroop task in identifying the changes in cognitive function due to fasting.
Anterior Mandibular Lingual Salivary Gland Defect Mimicking an Odontogenic Cyst- A Case Report
Authors: Anehosur Venkatesh , Radder Kiran , K. Harish , Hegde Veda 
Published: April 2020

AbstractStafne's bone defects (SBD) are usually asymptomatic and appears as a radiolucent lingual/buccal bone lesions of the lower jaw and are frequently caused by soft tissue inclusion. SBDs bone defects are considered to be an anatomic condition and most often seen in the posterior part the mandible below the mandibular canal. This is a case of anterior mandibular SBD, which was accidently detected in a patient with mandibular fracture.
A Review of Occlusal Registration Materials Utilized in Recording Various Occlusal Relations
Authors: Shetty Ganaraj , Shetty Manoj 
Published: April 2020

AbstractThe success of any prosthetic rehabilitation depends on several factors; most important factor is precisely recording of centric relation and transferring the same to the articulator. When partial or completely edentulous arches are present, cast mounting is considerably more complex. Interocclusal records are the means by which the interarch relationships are transferred from mouth to an articulator. The purpose of this review is to evaluate the accuracy and stability of the materials used in routine clinical practice to obtain interocclusal records.
Microcystic Serous Cystadenoma of Pancreas Presenting With Chronic Diarrhea
Authors: Kotian Shravya Sudesh , Bhat Shubha P , Mohan Rajshekhar , K. Sajitha 
Published: April 2020

AbstractPancreatic cystic neoplasms are increasingly being recognized due to widespread use of advanced imaging techniques. The prevalence of pancreatic cystic lesions is estimated to be between 2.6% - 19.6%. Serous Cystic Neoplasms account for nearly 16% of surgically resected th th pancreatic cystic neoplasms. They commonly occur in females between 5 and 6 decade of life. These tumours are essentially benign and asymptomatic unless they attain a large size. Majority of them occur in the body and tail of pancreas. Malignant transformation is reported in 1%-3% of cases. Surgical excision is the treatment of choice. Thus, it is important to accurately diagnose this lesion to avoid unnecessary intervention in the form of chemotherapy or uncalled for surgical resection. We report a rare case of a 65 year old male who presented with a history of intermittent diarrhea.
Awareness amongst Audiologists Regarding Effects of Radiation Therapy on Auditory System
Authors: Joseph Bennet Elsa , Vinod Nituna , Thomas Dona Maria , S. Arya Krishnan 
Published: April 2020

Abstract
Background: Radiation therapy has been a life saver for patients suffering from cancer. Hearing loss can be one of the many side effects of radiation therapy. It can affect the integration of the patient into society after the treatment of cancer is complete. Along with hearing loss, the patients may also experience other auditory symptoms like tinnitus, loudness intolerance and speech perception difficulties which are to be assessed, diagnosed and treated by Audiologists. In order to provide effective rehabilitation, it is necessary that Audiologists are aware of the effect of radiation therapy on the auditory system and what role they play in the interdisciplinary approach. The aim of the present study was to briefly assess whether this awareness is present amongst Audiologists.
Method : A questionnaire was prepared to comprise of questions related to the various aspects of effects of radiation therapy on the auditory system. The questionnaire was circulated online amongst Audiologists and completed questionnaires were subjected to descriptive statistical analysis.
Results : Only 76.9% Audiologists who were part of the survey were sure that hearing loss can be a side effect of radiation therapy. Many of the Audiologists were not sure whether they should provide audiological services to the patient before radiation therapy or after radiation therapy. 44% were not sure about what measures could be taken to reduce the effect of radiation therapy on the auditory system. 96.3% stated that there is a need to receive more specific training in dealing with rehabilitative cases post radiation therapy.
Tendencias en Reconstrucción en Ligamento Cruzado Anterior: Encuesta a 103 Cirujanos
Authors: Tuca María Jesús , Richard Javier , Espinoza Gonzalo 
Published: April 2020

Resumen
Objetivo Describir las tendencias en reconstrucción de LCA (R-LCA) entre los traumatólogos ejerciendo en Chile, delineando sus preferencias en cuanto a la técnica quirúrgica.
Método encuesta online de selección múltiple y recolección anónima de datos, dirigida a todos aquellos cirujanos que realizan R-LCA en Chile. Fue enviada por correo electrónico desde la Sociedad Chilena de Ortopedia y Traumatología a todos sus afiliados.
Resultados 103 cirujanos completaron la encuesta, realizando su práctica quirúrgica en Santiago (53%) y regiones (47%). En el 53% de los encuestados la cirugía de rodilla representaba más del 75% de su práctica, mientras que sólo en 9 ellas eran menos del 25%. El autoinjerto de isquiotibiales es utilizado en el 70% de R-LCA, seguido por el autoinjerto de tendón patelar (27%). En cuanto a la preparación de túneles, 33% utiliza técnica transtibial y 67% túneles independientes (13% fresado retrógrado, 54% portal medial). Para la fijación de injerto isquiotibiales, botones corticales es lo más utilizado en el fémur (73%), y tornillos interferenciales en la tibia (82%). Para la fijación femoral y tibial de injerto de tendón patelar, los tornillos metálicos son los más utilizados (75%). Un 41% realiza un pretensado del injerto, un 59% lo ciclan una vez fijo, y sólo 8 aplican una solución antibiótica sobre el injerto. La mayoría (89%) realiza la fijación en 10°-20° de flexión, ya sea con rotación externa (36%) o neutra (53%). En un 7% de las R-LCA primarias y en un 27% de las revisiones agregan una reconstrucción anterolateral.
Conclusión la tendencia en R-LCA en Chile es el uso de autoinjerto de isquiotibiales, con fresado independiente del túnel femoral y fijación en flexión con botones corticales y tornillos interferenciales.
Triple Pelvic Osteotomy Fixed with Lag Screw for the Treatment of Pelvic Canal Stenosis in Five Cats
Authors: Cinti Filippo , Cavaliere Leonardo , Degna Matteo Tommasini , Rossi Federica , Pisani Guido 
Published: April 2020

AbstractObjective The aim of this study was to describe the technique and the clinical outcome of cats with obstipation as a result of pelvic canal narrowing, treated using an alternative triple pelvic osteotomy technique.Methods Five cats with obstipation as a result of pelvic canal narrowing due to pelvic fracture malunion or secondary hyperparathyroidism were treated by triple pelvic osteotomy. After performing the iliac osteotomy and medial partial cortical debridement (4/5 cases), the pelvic canal was widened by moving the caudal fragment of the ilium to the lateral aspect of the cranial fragment. The iliac fragments were fixed by a 2.7-mm lag screw (5/5 cases) and an additional 2 Kirschner wires 0.8 mm (1/5 cases).Results The radiographic examination immediately postoperatively and 8 weeks postoperatively showed a mean pelvic canal enlargement of 20% (range 7–38%). Minor complication occurred in one case; this resolved 15 days postoperatively without any treatment. Complications and recurrence of obstipation did not occur during the final follow-up, ranging between 5 months and 1 year in any of these cases.Clinical Significance The use of lag screw fixed triple pelvic osteotomy resulted in widening of the pelvic canal and led to a satisfactory outcome with no major complications. This technique is fast, requires no expensive implants and it is relatively straightforward. Further cases are required to determine the success and complication risk of the procedure.
Combined Use of Gastric Aspirate and Induced Sputum Increases the Microbiological Diagnosis of Tuberculosis in Children
Authors: Atehortúa-Muñoz Santiago , Cardona-Moreno Andrea , Niño-Quiroga Laura , Contreras-Ortiz Javier , Arango-Ferreira Catalina 
Published: April 2020

Abstract
Objective Diagnosis of pulmonary tuberculosis (PTB) in pediatrics is a challenge due to the paucibacillary condition of the disease in this population, low sputum expectoration, and diverse unspecific symptomatology. Mycobacterial isolation through culture remains a priority. The objective of this study is to explore gastric aspirates and induced sputum techniques in the pediatric population for positivity on mycobacterial cultures.
Methods In this observational analytical study, two temporal groups were evaluated. A comparison of the isolation rate defined as positive culture confirmation by gastric aspirate (GA), induced sputum (IS), or combination of these both techniques in children under 10 years of age. The study included 86 children, 37 in the first evaluated study group and 49 in the second group.
Discussion Culture positivity was 10.8 and 30.6% for the first and second case series, respectively. These findings showed that the combination of GA and IS in two consecutive days yielded a significantly higher detection rate to confirm pulmonary tuberculosis by culture.
Conclusion The combination of GA plus IS samples for collection of M. tuberculosis culture can be a useful, nonexpensive, and safe diagnostic tool in low- and middleincome countries to diagnose tuberculosis in children.
Inferior Vena Cava Filter in Childhood Septic Deep Vein Thrombosis
Authors: Aljabari Salim , Waheed Shahzad , Davis Ryan , Padhye Amruta 
Published: April 2020

AbstractInferior vena cava (IVC) filter in venous thromboembolism (VTE) is an alternative to anticoagulation when the latter is contraindicated. The use of IVC filter in pediatrics continues to be rare and has not increased despite the ever-increasing rates of childhood VTE. Historically, septic VTE was regarded as a contraindication to IVC filter. Safety and efficacy of IVC filters in septic VTE have been reported in adult patients but not in pediatric patients. In this study, we reported a safe use of IVC filter in a critically ill 12-year-old patient with a large IVC thrombus and multiple pulmonary embolisms with favorable outcome.