bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasmodium infection prevents recurrence and metastasis of hepatocellular carcinoma potentially via inhibiting EMT
Authors: Liang Yun , Chen Xiao , Tao Zhu , Ma Meng , Adah Dickson , Li Xiaofen , Dai Lingling , Ding Wenting , Fanuel Songwe , Zhao Siting , Qin Li , Chen Xiaoping , Zhang Xiaowen 
Published: May 2020

Abstract
Background: Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer mortality worldwide and is characterized by a high rate of recurrence. We have previously reported Plasmodium infection inhibits tumor development and metastasis in a murine Lewis lung cancer model. In the current study, we aimed to examine the effects of Plasmodium infection on HCC metastasis and recurrence.Methods: Antitumor effects of Plasmodium infection were determined using two murine orthotopic HCC models, the non-resection model for investigating the effect of Plasmodium infection on liver tumor progression and metastasis, the resection model for investigating the effect of Plasmodium infection on the tumor recurrence after tumor was removed. Tumor tissues derived from tumor-bearing mice treated with or without Plasmodium infection were harvested after 15 days of tumor inoculation. The biomarkers related to epithelial-mesenchymal transition (EMT) and molecules associated with CCR10-mediated PI3K/Akt/GSK-3β/Snail pathway signaling were identified by qRT-PCR and western blot. Results: We found that Plasmodium infection significantly suppressed progression, recurrence and metastasis of HCC and prolonged the survival of tumor-bearing mice in both models. The expression levels of E-cadherin were significantly higher in Plasmodium treated group compared with those in control group, whereas the expression levels of Snail were significantly lower in the treated group than those in control group. Furthermore, Plasmodium infection inhibited the activation of Akt and GSK-3β in the tumor tissues by downregulating the expression of CCR10, thereby suppressing the accumulation of Snail and potentially contributed to the suppression of EMT and the prevention of tumor recurrence and metastasis.Conclusion: This study suggested that Plasmodium infection inhibited recurrence and metastasis, improved the prognosis of HCC potentially via suppression of CCR10‑mediated PI3K/Akt/GSK‑3β/Snail signaling, and prevention of EMT. These findings may be important in the development of novel therapy for HCC recurrence and metastasis especially for patients during the perioperative period.
Total Cholesterol Concentration Predicts the Effect of Plasmapheresis on Hypertriglyceridemic Acute Pancreatitis
Authors: chen zhu , Huang xiaolong , Han na , Guo Yanxia , Chen Jing , Ning yaogui , zhang minwei 
Published: May 2020

Abstract
Background The clinical efficacy of plasmapheresis (PP) in patients with hypertriglyceridemic acute pancreatitis (HLAP) might affected by total cholesterol (TC). The objective of this study is to determine the relationship of TC and the Triglyceride (TG)-lowing effect in patients on either non-PP or PP therapy.MethodsPatients were categorized into high total cholesterol (HTC) /low total cholesterol (LTC) groups based on TC level of 12.4 mmol/l. The primary outcome was TG reduction to below 500mg/dl within 48 hours. Linear mixed-effect model and logistic regression analyses were used to assess the association of TC and different therapy groups on TG-lowing efficacy.ResultsCompared with LTC group, patients with HTC showed more severe imaging manifestations(p<0.001) and higher APACH Ⅱ scores (p=0.036). Deaths occurred only in HTC groups. Significant interaction of time sequence with the 2 TGs-lowing therapy groups on TG level was only found in HTC group (p < 0.001). In patients with elevated TC level, primary outcome occurred in 66.67% of patients in the PP group, and 27.91% in the non-PP group. After adjustment for age, gender, CT grade and APACH II score, the odd ratio remain significant (OR 5.47, 95% confidence interval [CI] 1.84-16.25, p = 0.002). Furthermore, in patients with lower TC level, primary outcome occurred in 81.25% of patients in the PP group, and 62.30% in the non-PP group (adjusted OR 2.05; 95% CI 0.45–9.40; p = 0.353).Conclusions: TC could be a potential biomarker to predict the effects of TG-lowing therapy in patients with HLAP.
Prevalence and Correlates of Transactional Sex Among Women of Low Socioeconomic Status in Portland, Oregon
Authors: Menza Timothy W , Lipira Lauren , Bhattarai Amisha , Cali-DeLeon Victoria , Orellana Roberto 
Published: May 2020

Abstract
Background: Women who report transactional sex are at increased risk for HIV and other sexually transmitted infections (STIs). However,in the United States, social, behavioral, and trauma-related vulnerabilities associated with transactional sex are understudied and data on access to biomedical HIV prevention among women who report transactional sex is limited. Methods: In 2016, we conducted a population-based, cross-sectional survey of women of low socioeconomic status recruited via respondent-driven sampling in Portland, Oregon. We calculated the prevalence and,assessed the correlates of, transactional sex using generalized linear models accounting for sampling design. We also compared health outcomes,HIV screening, and knowledge and uptake of HIV pre-exposure prophylaxis (PrEP) between women who did and did not report transactional sex.Results: Of 334 women, 13.6% reported transactional sex (95% confidence interval [CI]: 6.8%, 20.5%). Women who reported transactional sex were older, more likely to identify as black, to identify as lesbian or bisexual, to experience childhood trauma and recent sexual violence, and to have been homeless. Six percent(95%CI: 1.8%, 10.5%) of women with no adverse childhood experiences (ACEs) reported transactional sex compared to 23.8%(95%CI: 13.0%, 34.6%)of women who reported eleven ACEs (P<0.001). Transactional sex was strongly associated withcombination methamphetamine and opiate use as well ascondomless sex. Women who reported transactional sex were more likely to report being diagnosed with a bacterial STI and hepatitis C; however, HIV screening and pre-exposure prophylaxis knowledge and use were low. Conclusions:In a sample ofwomen of low socioeconomic status in Portland, Oregon, transactional sex was characterized by marginalized identities, homelessness, childhood trauma, sexual violence, substance use, and sexual vulnerability to HIV/STI. Multi-level interventions that address these social, behavioral, and trauma-related factors and increase access to biomedical HIV prevention are critical to the sexual health of women who engage in transactional sex.
Influence of workload, mental health and professional quality of life on healthcare workers’ hand hygiene behavior in medical aid during COVID-19 pandemic
Authors: Zhou Qian , Zhang Xinping 
Published: May 2020

Abstract
BackgroundAppropriate hand hygiene behavior is much crucial during COVID-19 pandemic, which is influenced by workload, mental health and professional quality of life. The increasing workload, worse mental health and negative professional quality of life are severe challenges for healthcare workers, whereas the evidence of mechanism between them and hand hygiene behavior are limited. This study aimed to understand and model how HCWs’ hand hygiene behaviors were influenced by workload, mental health and professional quality of life.MethodsA total of 1,734 healthcare workers were investigated, covering self-reported hand hygiene behavior, mental health, workload, professional quality of life and socio-demographic characteristics. Structural equation modelling was conducted to confirm interrelationships and parameters among the variables.Findings The final model had a good fit (CFI=0.980; TLI=0.941; RMSEA=0.055). Higher workload was linked to worse mental health (β = 0.165, p < 0.001) and higher burnout (β = 0.183, p < 0.001). Worse mental health was linked to higher burnout (β = 0.339, p < 0.001). The higher burnout was associated with worse self-reported hand hygiene behavior (β = -0.302, p < 0.001).ConclusionWorkload is positively associated with mental health and burnout; mental health is positively related to burnout; burnout is negatively associated with hand hygiene behavior. Thus, hand hygiene behavior can be promoted by the strategy to decrease the workload, burnout and enhance mental health.
COVID-19 In Shang Hai: It is Worth Learning from the Successful Experience in Preventing and Controlling the Overseas Epidemic Situation
Authors: Dang Qi , Miao Rui , Yong Liang 
Published: May 2020

Abstract
BackgroundCOVID-19 first appeared in Wuhan, Hubei Province, China in late December 2019 and spread rapidly in China. Currently, the spread of local epidemics has been basically blocked. The import of overseas epidemics has become the main form of growth in China ’s new epidemic. As an important international transportation hub in China, Shanghai is one of the regions with the highest risk of imported cases abroad. Due to imported of overseas cases are affected by the international epidemic trend. The traditional infectious disease model is difficult to accurately predict the cumulative trend of cumulative cases in the Shanghai areas. It is also difficult to accurately evaluate the effectiveness of the international traffic blockade.MethodsIn this situation, this study takes Shanghai as an example to propose a new type of infectious disease prediction model. The model first uses the sparse graph model to analyze the international epidemic spread network to find countries and regions related to Shanghai. Next, multiple regression models were used to fit the existing COVID-19 growth data in Shanghai. Finally, the model predicts the growth curve of the COVID-19 epidemic in Shanghai without an international traffic blockade.ResultsIn this study, by constructing a sparse graph network model, 30 countries and regions related to Shanghai's overseas epidemic input were obtained, such as the United States. Moreover, the three regression models in this paper have obtained a good fitting effect. Finally, using data from 30 countries and regions related to Shanghai from April 4 to April 19 for a 15-day short-term forecast and comparing with real data, the results show that Shanghai’s international traffic blockade is effective and necessary.ConclusionThis research show that the control measures taken by Shanghai are very effective. At present, more and more countries and regions will face the current situation in Shanghai. We recommend that other countries and regions learn from Shanghai ’s successful experience in preventing overseas imports in order to fully prepare for epidemic prevention and control.
The influence of meteorology and air quality on parkrun athletic performance
Authors: Hodgson James Robert , Elliott Stephanie , Chapman Lee , Hudson Chris , Pope Francis D 
Published: May 2020

Abstract
Background

Despite increased awareness of climate change and urban air pollution, little research has been performed to examine the influence of meteorology and air quality on athletic performance of the general public and recreational exercisers. Anecdotal evidence of increased temperatures and wind speeds as well as higher relative humidity conditions resulting in reduced athletic performance has been presented in the past, whilst urban air pollution can have negative short- and long-term impacts on health. Furthermore, pollutants such as Ozone, Nitrogen Dioxide and Particulate Matter can cause respiratory and cardiovascular distress, which can be heightened during physical activity. Previous research has examined these impacts on marathon runners, or have been performed in laboratory settings. Instead, this paper focuses on the potential impacts on the general public. With the rise of parkrun events (timed 5 km runs) across the UK and worldwide concerns regarding public health in relation to both air quality and activity levels, the potential influence of air quality and meteorology on what is viewed as a ‘healthy’ activity has been investigated. A weekly dataset of parkrun participants at fifteen events, located in London UK, from 2011–2016 alongside local meteorological and air quality data has been analysed.
Results

The biggest influencer on athletic performance is meteorology, particularly temperature and wind speed. Regression results between parkrun finishing times and temperature predominantly show positive relationships, supporting previous laboratory tests. Increased relative humidity also causes slower finishing times but in several cases is not statistically significant. Higher wind speeds also result in slower times and in contrast to temperature and relative humidity, male participants are more influenced than female by this variable. Although air quality does influence athletic performance to an extent, the heterogeneity of pollutants within London and between parkrun events and monitoring sites makes this difficult to prove decisively.
Conclusions

It has been determined that temperature and relative humidity can have the largest detrimental impact on parkrun performance, with Ozone also having an impact. The influence of other variables cannot be discounted however and it is recommended that modelling is performed to further determine the extent to which ‘at event’ meteorology and air quality has on performance. In the future, there results can be used to determine safe operating and exercise conditions for parkrun and other public athletics events.
The HIV Epidemic In Colombia: Spatial And Temporal Trends Analysis﻿
Authors: Montana Jhon Freddy , Ferreira Glenda Roberta Oliveira Naiff , Cunha Carlos Leonardo Figueiredo , de Queiroz Ana Angelica Rego , Fernandes Wellington Augusto Andrade , Polaro Sandra Helena Isse , Gonçalves Lucia Hisako Takase , Couto Danielle Costa Carrara , Gir Elucir , Reis Renata Karina , Sorensen Wiliam , Botelho Elia Pinheiro 
Published: May 2020

Abstract
BACKGROUND – Colombia occupies 4th place among all Latin American countries in HIV/AIDS incidence rates, which have been increasing since the 1980s. There is a paucity of studies which address this trend. In this study we employed spatial and temporal trend analyses to study how the epidemic behaves in Colombian territory. METHODS - Our sample was composed of 72,994 HIV/AIDS cases and 21,898 AIDS-related deaths reported to the National Ministry of Health between 2008 and 2016. We employed the joinpoint regression model to analyze the annual HIV/AIDS incidence and AIDS mortality rates. In the spatial analysis we used univariate autocorrelation techniques and the Kernel density estimator. RESULTS – While the HIV/AIDS incidence had an increasing trend in Colombia, the AIDS mortality was stabilized. There was a downward trend in HIV/AIDS incidence and AIDS mortality among people between 0 to 14 years of age. An upward trend was observed for HIV/AIDS incidence in people older than 15 years of age and with the highest trend in 65 years and over group. AIDS mortality showed an increased trend in those 65 years old or over. The high-high clusters of HIV/AIDS incidence and AIDS mortality were located in the Andean and Caribbean regions. The HIV epidemic expanded from the North Caribbean region to Midwest Colombia.CONCLUSION – Sexual tourism and internal or external immigration might be responsible for the highest HIV/AIDS incidence and AIDS mortality rates observed in the Caribbean and Andean regions. Our results showed that Colombia needs to invest in specific and comprehensive public health policies to end HIV by 2030.
Development and validation of a nomogram to predict perioperative blood transfusion for gastric cancer surgery
Authors: Huang Haoquan , Han Zhixiao , Liang Xia , Liu Zhongqi , Cheng Shi , Cao Minghui 
Published: May 2020

Abstract
Background

This study aimed to construct and validate a nomogram composed of preoperative variables to predict perioperative blood transfusion for gastric cancer surgery.
Methods

600 gastric cancer patients undergoing gastrectomy between January 2010 and December 2015 were selected as primary cohort. 399 patients from January 2016 to June 2019 were collected as validation cohort. In the primary cohort, univariate and multivariate analyses were performed to identify independent risk factors for blood transfusion. Using Akaike information criterion, selected variables were incorporated to construct a nomogram. Validations of the nomogram were performed in the primary and validation cohort. Discrimination of the nomogram was assessed by the concordance index (C-index) and calibration of the nomogram was assessed by calibration curve and Hosmer–Lemeshow goodness-of-fit test.
Results

The following independent risk factors for transfusion were identified: Charlson comorbidity index score over 3 (odds ratio (OR) 2.15), tumor location (diffuse vs upper: OR 1.50), pTNM stage (III vs I: OR 3.17), type of gastrectomy (subtotal vs total gastrectomy: OR 0.58), extragastric organ resection (OR 2.03) and preoperative hemoglobin less than 80 g/l (vs over 120 g/l: OR 66.03). C-index was 0.863 and 0.901 in the primary and validation cohort, respectively, indicating good discrimination of the nomogram. Both calibration curves and Hosmer–Lemeshow goodness-of-fit tests (P-value 0.716 and 0.935) demonstrated high agreement between prediction and actual outcome.
Conclusion

A nomogram composed of preoperative variables to predict blood transfusion for gastric cancer surgery was developed and validated. This nomogram could be used to improve utilization of packed red blood cells.
Predictors of Failure on Second-line Antiretroviral Therapy with Protease Inhibitor Mutations in Uganda
Authors: Musana Hellen , Thaddeus Ssensamba Jude , Nakafeero Mary , Mugerwa Henry , Kiweewa Flavia Matovu , Serwadda David , Ssali Francis 
Published: May 2020

Abstract
Introduction Failure on second-line antiretroviral therapy (ART) with protease inhibitor (PI) mutations is on the rise. However, there is a paucity of information on the factors associated with this observation in the context of low-income countries. Knowledge of underlying factors is key if we are to minimize the number of PLHIV switched to costly third-line ART. Our study investigated the factors associated with failure on second-line ART with PI mutations. Methods We conducted a matched case-control analysis of patients' records kept at the Joint Clinical Research Center, starting from January 2008 to May 2018. We matched records of patients who failed the second-line ART with major PI mutations (cases) with records of patients who were virologically suppressed (controls) by a ratio of 1:3. Data analysis was conducted using STATA Version 14, and descriptive statistics comparing cases and controls were generated. Categorical variables were compared with the outcome, failure on second line ART with PI mutations using the Chi-square and Fisher's exact tests where appropriate. Conditional logistic regression for paired data was used to assess the association between the outcome and exposure variables, employing the backward model building procedure was done. Results Of the 340 reviewed patients' records, 53% were women, and 6.2% had previous Tuberculosis treatment. Males (aOR 2.64 CI: 1.0-4.64), type of second-line ART (aOR 3.92 CI: 1.15-13.38), and Tuberculosis treatment while on second-line ART (aOR7.08 CI: 2.35-21.29) highly predicted failure on second-line ART with PI mutations. Conclusion Males and patients concomitantly on Tuberculosis treatment while on second-line ART are at a higher risk of failing on second-line ART with PI mutations. HIV/AIDS response programs should give special attention to this group of people if we are to minimize the need for expensive third-line ART. More extensive explorative studies to ascertain underlying factors are recommended.
Distinct gut microbial communities and metabolic functions are associated with cachexia in lung cancer patients
Authors: Ni Yueqiong , Lohinai Zoltan , Heshiki Yoshitaro , Dome Balazs , Moldvay Judit , Dulka Edit , Gallfy Gabriella , Berta Judit , Weiss Glen , Sommer Morten , Panagiotou Gianni 
Published: May 2020

Abstract
Background

Cachexia is associated with decreased survival in cancer patients and has a prevalence of up to 80%. The etiology of cachexia is poorly understood, and limited treatment options exist. Here, we investigated the role of the human gut microbiome in the clinical setting by integrating shotgun metagenomics and plasma metabolomics of 38 lung cancer patients, with known cachexia status.
Results

The cachexia group showed significant differences in the gut microbial composition, functional pathways of the metagenome, and the related plasma metabolites compared to non-cachectic patients. Branched-chain amino acids (BCAAs), methylhistamine, as well as vitamins, were significantly depleted in the plasma of cachexia patients, which was also reflected in the depletion of relevant gut microbiota functional pathways. The enrichment of plasma BCAAs and 3-oxocholic acid in non-cachectic patients were positively correlated with the gut microbial species Prevotella copri and Lactobacillus gasseri, respectively. Furthermore, the gut microbiota capacity for lipopolysaccharides biosynthesis was significantly enriched in the cancer cachectic patients. The involvement of gut microbiome in cachexia was further observed in a high-performance machine learning model that uses solely gut microbial taxonomic and pathway features to differentiate cachectic from non-cachectic cancer patients.
Conclusions

Our study demonstrates the links between host metabolism and specific gut microbial species and functions in a clinical setting, suggesting that the gut microbiota could have an influence on cachexia with possible future therapeutic applications.
Fermented Astragalus in diet improved laying performance, egg quality, antioxidant and immunological status and intestinal microbiota in laying hens
Authors: Shi Hongtao , Wang Baiyu , Bian Chuanzhou , Han Yingqian , Qiao Hongxing 
Published: May 2020

Abstract
In the era of increased antibiotic resistance and ever stricter control on antibiotic use, it is urgent to develop green, safe and non-residue alternatives to antibiotics applied to the poultry industry. To this end, we supplied the potential Lactobacillus Plantarum (L. Plantarum) fermented Astragalus in the diet of laying hens, with a final addition of 3‰. Its effects have been assessed on laying performance, egg quality, antioxidant and immunological status and intestinal microbiota, and are compared to the control group, to the Astragalus group containing 3‰ unfermented Astragalus, and to the L. Plantarum group containing 2% L. Plantarum (1 × 108 CFU/mL). During the second half of the experimental period (15 to 28 days), the egg production rate was significantly higher in the fermented Astragalus group than that in the other groups, with the fermented Astragalus group having the lowest feed conversion ratio. No significant difference (P > 0.05) was observed among treatments on egg quality. Fermented Astragalus-treated hens exhibited significantly increased catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum, and reduced malondialdehyde (MDA) in serum. Furthermore, fermented Astragalus supplementation resulted in a significant increase in ileal microbiota abundance relative to control. In conclusion, feeding laying hens with L. Plantarum fermented Astragalus has beneficial effects on production, antioxidant potential, immunity and ileal microbiota. L. Plantarum fermented Astragalus is expected to be a novel feed additive used in poultry production.