bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGARUH KONSENTRASI STARTER Saccharomyces cerevisiae DAN LAMA FERMENTASI TERHADAP RENDEMEN DAN MUTU MINYAK KELAPA
Authors: BARLINA RINDENGAN , KAROUW STEIVIE , PASANG PATRIK M. 
Published: July 2020

<p>Untuk mendapatkan cara pengolahan minyak kelapa yang lebih eisien dalam menghasilkan rendemen dan mutu yang tinggi dan tahan simpan serta aman dikonsumsi telah dilakukan penelitian pengaruh konsentrasi starter Saccharomyces cerevisiae dan lama fermentasi terhadap rendemen mutu minyak kelapa. Penelitian dilaksanakan di Laboratorium Balai Penelitian Tanaman Kelapa dan Palma Lain Manado dan Kebun Percobaan Mapanget sejak bulan Maret sampai Desember 2001. Penelitian menggunakan rancangan acak lengkap disusun secara faktorial, yaitu faktor A adalah konsentrasi starter Saccharomyces cerevisiae, terdiri dari 0%, 0.25%, 0.35%, 0.45% dan faktor B adalah lama fermentasi krim. terdiri dari 0 jam, 12 jam, dan 24 jam. Ulangan dilakukan sebanyak 2 kali. Hasil penelitian diperoleh rendemen minyak tertinggi 23.83% pada fermentasi krim selama 24 jam. Mutu minyak kelapa yang dihasilkan sebagai berikut kadar air 0.03 - 0.18%, asam lemak bebas 0.15 - 0.29%, warna bening dan bau harum/ normal, bilangan peroksida berkisar 0.20 - 0.40 meq/kg, mutu minyak kelapa tersebut memenuhi Standar Nasional Indonesia (SNI) 01-2902-1992.</p><p>Kata kunci: Minyak kelapa, Saccharomyces cerevisiae, konsentrasi, fermentasi. rendemen, mutu</p><p> </p><p><strong>ABCTRACT </strong></p><p><strong>Effect of concentration of stater Saccharomyces cerevisiae and duration of fermentation on the content and quality of coconut oil</strong></p><p>Research on the effect of concentration of starter Saccharomyces cerevisiae on the content and quality of coconut oil was conducted at the Laboratory of Indonesian Coconut and Palmae Research Institute (ICOPRI) and Mapanget Research Instalation from March to December 2001. The objective of the research was to find out the effective technique and processing of coconut oil. The research used a completely randomized design with 2 factors and 2 replications. Factor A was the concentration of starter Saccharomyces cerevisiae of 0%, 0.25%, 0.35%, 0.45% and factor B was duration of fermentation consist of 0 hour, 12 hours, and 24 hours. The results showed that the highest yield of coconut oil is 23.83% was obtained by fermented coconut cream for 24 hours. The coconut oil had good quality with moisture content about 0.03 to 0.18%, free fatty acid content about 0.15 to 0.29%, colorless, good smell and peroxide value about 0.20 - 0.40 mcq/kg, the quality of coconut oil fulfilled the requirements of Indonesian National Standard (Standar Nasional Indonesia/SNI 01-2902-1992).</p><p>Key words: Coconut oil, Saccharomyces cerevisiae. concentration, fermentation, yield and quality</p>
STABILITAS HASIL BEBERAPA GALUR JARAK
Authors: MARDJONO RUSIM , SUDARMO HADI , SUPRIJONO . 
Published: July 2020

<p>Penelitian mi dilaksanakan di dua lokasi yaitu di Sandubaya (l-ombok Timur. NTB) dan Bayan (I-ombok Barat, NTB) dilakukan pada musim lanam 1998/1999 dan 1999/2000 bulan Nopember 1998 sampai dengan September 2000. Penelitian ini bertujuan unluk mem¬ peroleh galur-galur unggul jarak bcrproduktivitas tinggi, dan spcsiik di Kawasan Timur Indonesia. Galur yang diuji sebanyak 9 galur harapan yaitu RC.67, RC.74. RC.86, RC.104, RC.106, RC.220, RC.221, RC.64, KF.VIII dan sebagai pembanding digunakan 3 varietas komersial yaitu Asembagus 22, Asembagus 60 dan Asembagus 81. Penelitian mengguna¬ kan rancangan acak kelompok dengan ulangan 3 kali. Hasil penelitian menunjukkan 3 galur unggul yaitu RC.67, RC.221 dan RC.64, yang sama potensi dengan varietas Asb.81, mempunyai adaptasi luas (varietas stabil). Dua galur lainnya yaitu RC.74 dan RC.104 beradaplasi sempit (spesiik lokasi) sesuai untuk daerah Bayan Nusa Tenggara Barat</p><p>Kata kunci : Ricmus commums, stabilitas hasil, spesifik lokasi</p><p> </p><p><strong>ABSTRACT </strong></p><p><strong>Yield Stability of Castor Lines</strong></p><p>The experiment was conducted in two locations at Sandubaya (East I-ombok) and Bayan (West lombok), in planting session 1998/1999 and 1999/2000 from November 1998 to September 2000 This experiment was aimed to ind oul superior lines of castor, high productivities, that can be developed in speciic dry area in easten pat of Indonesia. The trial was arranged in randomized block design with 3 replications. Nine lines, RC.67, RC.74, RC.86, RC.104. RC.106. RC.220, RC. 221, RC6I, KF.VIII and 3 control varieties, Asembagus 22, Asembagus 60 and Asembagus 81 were used as treatment. Results of this experiment found out 3 superior lines: RC 67, RC.221 and RC.64, which have the same potential with Asb.81 variety, have broad adaptation (stable lines). Two other lines : RC.74 and RC.104 have narrow adaptation (speciic location) just for Bayan. West Nusa Tenggara.</p><p>Key words: Ricmus commums, yield stability, specific location</p>
Impacto de la ley de apoyo a los emprendedores y a su internacionalización. Nuevos criterios de aplicación.
Authors: Ortega Giménez Alfonso , Castellanos Cabezuelo Ángela 
Published: July 2020

Hasta nueve Ministerios participan en la elaboración de uno de los proyectoslegislativos más emblemáticos del Gobierno del Presidente Rajoy (la Ley 14/2013, de 27 de septiembre, de apoyo a los Emprendedores y su Internacionalización) que revisa toda la normativa relativa al empleo y al emprendedurismo y afecta tanto al diseño de las materias en la Educación Primaria como a la política de concesión de visados de residencia del Gobierno.El conjunto de medidas que prevé la Ley pretende, entre otros, atraer inversores, emprendedores, trabajadores que efectúen movimientos intraempresariales, profesionales altamente cualificados e investigadores. Las medidas implementadas en la Ley, en definitiva, permitirán aumentar la competitividad de la economía de nuestro país, puesto que incentivará la internacionalización de las empresas. Este articulo pretende, de una forma sencilla, ágil y con un enfoque práctico, analizar los nuevos criterios de aplicación de la Ley 14/2013, de 27 de septiembre, de Apoyo a los Emprendedores y su Internacionalización [Ley 14/2013, de 27 de septiembre, de apoyo a los emprendedores y su internacionalización (BOE núm. 233, de 28 de septiembre de 2013)].
IMPACTO DA MONOCULTURA DA SOJA NA AGRICULTURA CAMPONESA: UM ESTUDO SOBRE AS MUDANÇAS NA PRODUÇÃO DE LEITE NO ASSENTAMENTO ITAMARATI I – FETAGRI/MS
Authors: Camacho Rodrigo Simão , Almeida Denise Camilo de , Conceição Cristiano Almeida da 
Published: July 2020

Neste artigo, propomos estudar e responder algumas questões sobre a situação em que se encontra a forma de trabalho dos assentados na FETAGRI grupo sete Bacia Leiteira no assentamento Itamarati I - MS. O motivo pelo qual nos propomos a fazer a pesquisa foi para entender como pensam as pessoas que estão envolvidas nesse processo, qual é o impacto que a produção da monocultura da soja tem causado no grupo e o que tem levado os produtores a seguirem por este caminho. Em nossa metodologia fizemos um trabalho de campo com um roteiro de perguntas que facilitou a conversa com os entrevistados. Consideramos que este estudo será relevante para as pessoas que se interessam pelo debate da produção familiar e para entender a influência do agronegócio no assentamento Itamarati, Ponta Porã-MS.
ENTREVISTA COM PROFESSOR LUIZ GONZAGA FALCÃO VASCONCELLOS
Authors: Oliveira Neto Thiago , Braga Eduardo Henrique Freitas , Vasconcellos Luiz Gonzaga Falcão 
Published: July 2020

Entrevista realizada pela Internet, no dia 14.11.2018 – Manaus / AM e Uberlândia / MG. Entrevistado: Luiz Gonzaga Falcão Vasconcellos
Texto da entrevista foi revida pelo entrevistado, no período de março a maio de 2020.
Icariside I specifically facilitates ATP or nigericin-induced NLRP3 inflammasome activation and causes idiosyncratic hepatotoxicity
Authors: Gao Yuan , Bai Zhaofang , Xiao Xiaohe , Xu guang , Niu ming , Wang jiabo , Zhan xiaoyan , Guo yuming , Wang zhilei , Ma li , He tingting , Qin nan , Shi wei 
Published: July 2020

Abstract
Background: Epimedii Folium(EF) is commonly used for treating bone fractures and joint diseases, but the potential hepatotoxicity of EF limits its clinical application. Our previous study confirms that EF could lead to idiosyncratic drug-induced liver injury (IDILI) and hepatocyte apoptosis, but the mechanism remains unknown. Studies have shown that NLRP3 inflammasome plays an important role in the development of various inflammatory diseases such as IDILI. Specific stimulus-induced NLRP3 inflammasome activation may has been a key strategy for lead to liver injury. Therefore, main compounds derived from EF were chosen to test whether the ingredients in EF could activate the NLRP3 inflammasome and to induce IDILI.Methods: Mouse were treated with Icariside I, and then stimulated with inflammasome stimuli and assayed for the production of caspase-1 and interleukin 1β (IL-1β) and the release of lactate dehydrogenase (LDH). Determination of intracellular potassium, ASC oligomerization as well as reactive oxygen species (ROS) production were used to evaluate the stimulative mechanism of Icariside I on inflammasome activation. Mouse models of NLRP3 diseases were used to test whether Icariside I has hepatocyte apoptosis effects and promoted NLRP3 inflammasome activation in vivo.Results: Icariside I specifically enhances NLRP3 inflammasome activation triggered by ATP or nigericin but not SiO2, poly(I:C) or cytosolic LPS. Additionally, Icariside I does not alter the activation of NLRC4 and AIM2 inflammasomes. Mechanically, Icariside I alone does not induce mitochondrial reactive oxygen species (mtROS), which is one of the critical upstream events of NLRP3 inflammasome activation; however, Icariside I increases mtROS production induced by ATP or nigericin but not SiO2. Importantly, Icariside I leads to liver injury and NLRP3 inflammasome activation in an LPS-mediated susceptibility mouse model of IDILI, but the effect of Icariside I is absent in the LPS-mediated mice model pretreated with MCC950, which is used to mimic knockdown of NLRP3 inflammasome activation.Conclusions: Our study reveals that Icariside I specifically facilitates ATP or nigericin-induced NLRP3 inflammasome activation and causes idiosyncratic hepatotoxicity. The findings suggest that Icariside I or EF should be avoided in patients with diseases related to ATP or nigericin-induced NLRP3 inflammasome activation, which may be risk factors for IDILI.
Distinct evolutionary trajectories of V1R clades across mouse species
Authors: Miller Caitlin H , Campbell Polly , Sheehan Michael J 
Published: July 2020

Abstract
BACKGROUND: Many animals rely heavily on olfaction to navigate their environment. Among rodents, olfaction is crucial for a wide range of social behaviors. The vomeronasal olfactory system in particular plays an important role in mediating social communication, including the detection of pheromones and recognition signals. In this study we examine patterns of vomeronasal type-1 receptor (V1R) evolution in the house mouse and related species within the genus Mus . We report the extent of gene repertoire turnover and conservation among species and clades, as well as the prevalence of positive selection on gene sequences across the V1R tree. By exploring the evolution of these receptors, we provide insight into the functional roles of receptor subtypes as well as the dynamics of gene family evolution.

RESULTS: We generated transcriptomes from the vomeronasal organs of 5 Mus species, and produced high quality V1R repertoires for each species. We find that V1R clades in the house mouse and relatives exhibit distinct evolutionary trajectories. We identify putative species-specific gene expansions, including a large clade D expansion in the house mouse. While gene gains are abundant, we detect very few gene losses. We describe a novel V1R clade and highlight candidate receptors for future study. We find evidence for distinct evolutionary processes across different clades, from largescale turnover to highly conserved repertoires. Patterns of positive selection are similarly variable, as some clades exhibit abundant positive selection while others display high gene sequence conservation. Based on clade-level evolutionary patterns, we identify receptor families that are strong candidates for detecting social signals and predator cues. Our results reveal clades with receptors detecting female reproductive status are among the most conserved across species, suggesting an important role in V1R chemosensation.

CONCLUSION: Analysis of clade-level evolution is critical for understanding species’ chemosensory adaptations. This study provides clear evidence that V1R clades are characterized by distinct evolutionary trajectories. As receptor evolution is shaped by ligand identity, these results provide a framework for examining the functional roles of receptors.
Glutamate-Glutamine Homeostasis is Perturbed in Neurons and Astrocytes derived from Patient iPSC Models of Frontotemporal Dementia
Authors: Aldana Blanca Irene , Zhang Yu , Jensen Pia , Chandrasekaran Abinaya , Christensen Sofie Kjellerup , Nielsen Troels Tolstrup , Nielsen Jørgen , Hyttel Poul , Larsen Martin Røssel , Waagepetersen Helle , Freude Kristine Karla 
Published: July 2020

Abstract
Frontotemporal dementia (FTD) is amongst the most prevalent early onset dementias and even though it is clinically, pathologically and genetically heterogeneous, a crucial involvement of metabolic perturbations in FTD pathology is being recognized. However, changes in metabolism at the cellular level, implicated in FTD and in neurodegeneration in general, are still poorly understood. Here we generate induced human pluripotent stem cells (hiPSCs) from patients carrying mutations in CHMP2B (FTD3) and isogenic controls generated via CRISPR/Cas9 gene editing with subsequent neuronal and glial differentiation and characterization. FTD3 neurons show a dysregulation of glutamate-glutamine related metabolic pathways mapped by 13 C-labelling coupled to mass spectrometry. FTD3 astrocytes show increased uptake of glutamate whilst glutamate metabolism is largely maintained. Using quantitative proteomics and live-cell metabolic analyses, we elucidate molecular determinants and functional alterations of neuronal and glial energy metabolism in FTD3. Importantly, correction of the mutations rescues such pathological phenotypes. Notably, these findings implicate dysregulation of key enzymes crucial for glutamate-glutamine homeostasis in FTD3 pathogenesis which may underlie vulnerability to neurodegeneration.
A comparison of four different models of acute respiratory distress syndrome in sheep
Authors: Engel Monique , Nowacki Relana M.E. , Jonker Elly M. , Ophelders Daan , Nikiforou Maria , Kloosterboer Nico , Zimmermann Luc J.I. , Waardenburg Dick A. van , Kramer Boris W. 
Published: July 2020

Abstract
Background: Acute respiratory distress syndrome (ARDS) can have various causes. The study objective was to investigate whether different pathophysiologic models of ARDS would show different respiratory, cardiovascular and inflammatory outcomes. Methods: We performed a prospective, randomized study in 27 ventilated ewes inducing ARDS using three different techniques to mimic the pulmonary causes of ARDS (ARDSp): warm saline lavage (n=6), intratracheal hydrochloric acid (HCl; n=6), intratracheal albumin (n=10), and one technique to mimic an extrapulmonary cause of ARDS (ARDSexp): intravenous lipopolysaccharide (LPS iv; n=5). ARDS was defined when PaO 2 was <15kPa (112 mmHg) when ventilated with PEEP 10cm H 2 O and FiO 2 =1.0. The effects on gas exchange were investigated by calculating the oxygenation index (OI) and the ventilation efficacy index (VEI) every 30 minutes for a period of 4 hours. Post mortem lung lavage was performed to obtain broncho-alveolar lavage fluid (BALF) to assess lung injury and inflammation. Lung injury and inflammation were assessed by measuring the total number and differentiation of leukocytes, the concentration of protein and disaturated phospholipids, and interleukine-6 and -8 in the BALF. Histology of the lung was evaluated by measuring the mean alveolar size, alveolar wall thickness and the lung injury score system by Matute-Bello et al., as markers of lung injury. The concentration of interleukin-6 was determined in plasma, as a marker of systematic inflammation. Results: The OI and VEI were most affected in the LPS iv group and thereafter the HCl group, after meeting the ARDS criteria. Diastolic blood pressure was lowest in the LPS iv group. There were no significant differences found in the total number and differentiation of leukocytes, the concentration of protein and disaturated phospholipids, or interleukin-8 in the BALF, histology of the lung and the lung injury score. IL-6 in BALF and plasma was highest in the LPS iv group, but no significant differences were found between the other groups. It took a significantly longer period of time to meet the ARDS criteria in the LPS iv group. Conclusions: The LPS model caused the most severe pulmonary and cardiovascular insufficiency. Surprisingly, there were limited significant differences in lung injury and inflammatory markers, despite the different pathophysiological models, when the clinical definition of ARDS was applied.
Detection of gray matter microstructural changes in Alzheimer’s disease continuum using fiber orientation
Authors: Lee Peter , Kim Hang-Rai , Jeong Yong 
Published: July 2020

Abstract
Background: This study aimed to investigate feasible gray matter microstructural biomarkers with high sensitivity for early Alzheimer’s disease (AD) detection. We propose a diffusion tensor imaging (DTI) measure, “radiality”, as an early AD biomarker. It is the dot product of the normal vector of the cortical surface and primary diffusion direction, which reflects the fiber orientation within the cortical column. Methods: We analyzed neuroimages from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) database, including images from 78 cognitively normal (CN), 50 early mild cognitive impairment (EMCI), 34 late mild cognitive impairment (LMCI), and 39 AD patients. We then evaluated the cortical thickness (CTh), mean diffusivity (MD), which are conventional AD magnetic resonance imaging (MRI) biomarkers, and the amount of accumulated amyloid and tau using positron emission tomography (PET). Radiality was projected on the gray matter surface to compare and validate the changes with different stages alongside other neuroimage biomarkers.Results: The results revealed decreased radiality primarily in the entorhinal, insula, frontal, and temporal cortex with further progression of disease. In particular, radiality could delineate the difference between the CN and EMCI groups, while the other biomarkers could not. We examined the relationship between radiality and other biomarkers to validate its pathological evidence in AD. Overall, radiality showed a high association with conventional biomarkers. Additional ROI analysis revealed the dynamics of AD-related changes as stages onward.Conclusion: Radiality in cortical gray matter showed AD-specific changes and relevance with other conventional AD biomarkers with high sensitivity. Moreover, radiality could identify the group differences seen in EMCI, representative of changes in early AD, which supports its superiority in early diagnosis compared to that possible with conventional biomarkers. We provide evidence of structural changes with cognitive impairment and suggest radiality as a sensitive biomarker for identifying early AD.
Growth hormone alleviates oxidative stress and improves the IVF outcomes of poor ovarian responders: A randomized controlled trial
Authors: Gong Yan , Zhang Kun , Xiong Dongsheng , Wei Jiajing , Tan Hao , Qin Shengfang 
Published: July 2020

Abstract
Background: Oxidative stress (OS), defined as an imbalance between excessive reactive oxygen species (ROS) and/or reactive nitrogen species (RNS) production and antioxidant insufficiency, has been suggested to be involved in the pathogenesis of poor ovarian response (POR). Growth hormone (GH) can reduce OS in some cell types. This study investigated whether GH can improve OS and the in vitro fertilization and embryo transfer (IVF-ET) outcomes of poor ovarian responders.Methods: This study enrolled 105 patients with POR and 58 patients without POR (controls) who were diagnosed according to the Bologna criteria and underwent conventional IVF-ET. Poor ovarian responders were randomly assigned to two groups: the POR-GH group, which received pretreatment with GH 4 IU/d on day 2 of the previous menstrual cycle before IVF until the trigger day, and the POR-C group, which received no pretreatment. OS markers in follicular fluid (FF), ROS levels in granulosa cells (GCs), and the IVF outcomes of the groups were compared.Result(s): Endometrial thickness on trigger day, the number of cleaved embryos, the number of higher-quality embryos, and the rates of embryo formation, higher-quality embryo formation, implantation and clinical pregnancy were significantly increased in the POR-GH group compared with the POR-C group (P < 0.05). Moreover, compared to those in the non-POR group, FF malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI) and ROS levels in GCs were significantly higher, whereas superoxide dismutase (SOD) and the total antioxidant capacity (TAC) were significantly lower in the POR-C group (P < 0.05). Furthermore, compared with those in the POR-C group, the FF TAC was significantly increased in the POR-GH group, and TOS, OSI and intracellular ROS levels were significantly reduced (P < 0.05).Conclusion(s): Pretreatment with GH alleviates OS and improves oocyte quality and IVF outcomes of poor ovarian responders.Clinical Trial Registration: Chinese Clinical Trial Registry. ChiCTR1900021269. Registered 8 February 2019, http://www.chictr.org.cn/edit.aspx?pid=35837&htm=4.