bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Poor Prognostic Metastatic Nongestational Choriocarcinoma of the Ovary: A Case Report and the Literature Review.
Authors: Nishino Kimihiro , Yamamoto Eiko , Ikeda Yoshiki , Niimi Kaoru , Yamamoto Toshimichi , Kajiyama Hiroaki 
Published: October 2020

Abstract
Background: Pure ovarian choriocarcinoma can be gestational or nongestational in origin. Nongestational pure ovarian choriocarcinoma is extremely rare and the prognosis is thought to be worse than that of the gestational type in patients with metastatic disease. We present a case of metastatic pure ovarian choriocarcinoma with poor prognosis in which the origin was identified as nongestational by DNA short tandem repeat (STR) analysis. Case presentation: A nulliparous woman in her thirties with metastatic choriocarcinoma was referred to our hospital after initial treatment proved unsuccessful. Two months earlier, she had undergone brain tumor resection and histological examination confirmed choriocarcinoma. Serum human chorionic gonadotropin (hCG) concentration at initial diagnosis was 5030 IU/L. Two cycles of a combination chemotherapy regimen of methotrexate, etoposide, and actinomycin-D (MEA therapy), which is commonly used for gestational choriocarcinoma, was administered. However, the disease could not be controlled. Imaging modalities at presentation revealed tumor present in the left ovary and left lung, but not in the uterus, which led us think that the choriocarcinoma was nongestational. Bleomycin, etoposide, and cisplatin (BEP therapy) which is commonly used for nongestational choriocarcinoma (malignant germ cell tumor) and surgical resection of the uterus, bilateral ovaries, and an affected part of the left lung led to the nadir level of hCG, but the tumor relapsed and levels of hCG again increased. To investigate the origin of choriocarcinoma, we performed DNA STR analysis of tumor cells and oral mucosal cells. Analysis revealed the origin of the choriocarcinoma as nongestational, as the genotype of tumor cells entirely corresponded with that of oral mucosal cells. BEP therapy and chemotherapy regimens administered for nongestational choriocarcinoma and gestational choriocarcinoma proved ineffective, and the patient died 21 months after diagnosis of metastatic choriocarcinoma.Conclusion: Metastaic nongestational pure choriocarcinoma of ovary is an extremely rare and an aggressive disease, frequently resulting in poor outcome.
Association of Urinary Biomarkers Noninvasively Forecasts The Extent of Renal Injury In The Early Diabetic Nephropathy Patients With Kidney Qi Deficiency Syndrome: A Retrospective Investigation&nbsp;
Authors: Wu Wei , Meng Xian-Jie , Liu Ying-Lu , Wang Wen-Wen , Fang Qi-Jun , Yee Hong-Yun , Yuan Can-Can , Wang Mei-Zi , Chong Fee-Lan , Wan Zi-Yue , Wan Yi-Gang , Shen Shan-Mei 
Published: October 2020

Abstract
Background: Recently, diabetic nephropathy (DN) becomes a common health problem in China as one of the major microvascular complications of diabetes mellitus. Therefore, the incipient diagnosis and noninvasive detections in clinic by the association of urinary biomarkers are important for preventing the progress of DN. However, the clinical significance of urinary biomarkers is controversial nowadays. This study thereby aimed to further evaluate the clinical significance of the association of urinary biomarkers in noninvasively predicting the renal damaged extent of the early type 2 DN patients with kidney qi deficiency syndrome in an integrated traditional and western medical center, and preliminarily confirm the correlation between urinary tubular biomarkers and the biological bases of DN patients.Methods: Ninety-two patients in an integrated traditional and western medical center of China were categorized into 3 groups, 20 patients with normo-albuminuria, 50 patients with micro-albuminuria, and 22 patients with macro-albuminuria. In addition to urinary albumin (UAlb) and urinary albumin-to-creatinine ratio (UACR), serum creatinine, estimated glomerular filtration rate and various urinary tubular biomarkers were tested respectively. Besides, clinical characteristics and kidney asthenia syndrome distribution in all patients were observed. Results: In these 3 groups, 24-h UAlb and UACR showed stepwise and significant increases. Urinary cystatin C (UCysC), urinary N-acety1-β-D-glucosaminidase (UNAG) and urinary retinal binding protein (URBP) synchronously showed gradual increases consistent with albuminuria degree in 3 groups. Moreover, 24-h UAlb and UACR were positively correlated with UCysC, UNAG and URBP. In the 72 DN patients with albuminuria, there was a positive correlation between UNAG and URBP, and UCysC was also positively correlated with UNAG and URBP. Additionally, TCM syndrome distributional characteristics in all patients were consistent with the clinical manifestations of kidney qi deficiency syndrome. Conclusion: In this investigation, we ulteriorly demonstrated that the association within UCysC, UNAG, URBP and UAlb may be used as practical targets in noninvasively forecasting the extent of renal injury in the early type 2 DN patients with kidney qi deficiency syndrome. More importantly, we found that urinary tubular biomarkers may be one of the biological bases of DN patients with the specific traditional Chinese medicine syndrome.
Correlations Between Traditional Chinese Medicine Syndromes and Clinical Indexes in Health Check-Up Population
Authors: Liao Pei-Hung , Tu Chia-Ling , Liu Chi-Feng 
Published: October 2020

Abstract
BackgroundRoutine health check-up is associated with improved lifespan and reduced medical cost. Traditional Chinese medicine (TCM) serves as a cost-effective modality in healthcare system. We examined the correlations of TCM syndromes with modern medical indicators in health check-up population.MethodsWe studied 5231 subjects undergoing health check-up between January 1st 2008 and December 31, 2016. Physical indexes such as body weight and blood pressure and biomedical indicators like live function and tumour markers were measured. All subjects underwent colonoscopy. All subjects were classified and differentiated into five different TCM syndromes. An artificial neural network (ANN) was employed to evaluate the predictive value of TCM syndrome differentiation.ResultsOf enrolled subjects, SADH accounted for 85.8% and IDSIBSB was found in 4576 subject (87.5%). YaDSK and YiDLK accounted for 99.5% (5207) and 80.9% (4232) respectively. We found that YiDLK is correlated with abnormality of liver function indexes. The results showed that SADH is correlated with level of cholesterol in health check-up population. The results showed that the predictive ANN model showed a good fitting with an accuracy of 100%.ConclusionThe results demonstrated that TCM syndromes were closely correlated with clinical laboratory indexes regardless of health status. TCM syndrome differentiation is suggested to contribute to routine health examination as screening measure with its non-invasive nature.
Berbamine Inhibits Cell Proliferation, Migration, and Invasion and Induces Cell Apoptosis of A549 Cells Via Regulating C-Maf, PI3K/Akt and MDM2-P53 Pathways
Authors: Liu Lili , Xu Zhiying , Yu Binbin , Tao Li , Cao Ying 
Published: October 2020

Abstract
Background To investigate the influences of berbamine (BBM) on the cell viability, proliferation, and migration of A549 cells in vitro and in vivo, and explore the possible mechanisms.MethodsAfter the A549 cells were treated with BBM, the cell viability and proliferation of the cancer cells were detected by MTT assay, EdU assay, and colony formation assay. Migration and invasion of cancer cells were illustrated through wound scratch assay and transwell assay. Apoptosis of cancer cells was evaluated by trypan blue dye exclusion assay and elisa assay. Beside, the expression of PI3K/Akt signal pathway-related proteins and c-Maf were detected employing western blotting assay. Xenografted model of NSCLC was used to detect the effect of BBM on tumor growth and metastasis in vivo.ResultsMTT assay showed that BBM inhibited the viability of A549 cells in a concentration-dependent manner and time-dependent manner. The results from the colony formation assay and EdU assay revealed that BBM (10 µM) could significantly inhibit the proliferation of A549 cells (P＜0.001). And BBM (10 µM) significantly inhibited the migration and invasion in the wound scratch assay and transwell assay (P＜0.05). Trypan blue assay and elisa assay indicated that BBM (20 µM) significantly induced apoptosis of A549 cells. The nude mice assay manifested the tumor volume was significantly shrank by BBM (20 mg/kg) (P＜0.05). Western blotting assay showed that the PI3K/Akt and MDM2-p53 signaling pathways were inhibited by BBM, and the expression of c-Maf was downregulated by BBM. ConclusionsBBM could inhibit the proliferation and metastasis, and induce apoptosis of A549 cells in vitro and in vivo, these effects may be achieved by reducing the expression of c-Maf and regulating the PI3K/Akt and MDM2-p53 pathways.
First Systematic Analysis for Chemical Composition by HPLC-ESI-MSn and Antioxidant Activity Comparison of Dendrobium huoshanense and Dendrobium officinale
Authors: Wan Jingqiong , Gong Xiaohui , Wen Chongwei , Wei Yuan , Kong Lingdong , Han Bangxing , Ouyang Zhen 
Published: October 2020

Abstract
Background: Dendrobium huoshanense C. Z. Tang et S. J. Cheng (D. huoshanense) is a precious Dao-di herb with high quality, excellent efficacy and edible value of China, Dendrobium officinale Kimura et Migo (D. officinale) is widely utilized and commonly used as a substitute for D. huoshanense. But few studies have been conducted to systematically analyze chemical composition and bioactivities of the two.Methods: HPLC-ESI-MSn was used to identify the compounds in D. huoshanense and D. officinale grown in Huoshan from methanol extracts and different polar fractions of water extracts, including petroleum ether, ethyl acetate and n-butanol; The in vitro antioxidant activity were evaluated by the scavenging effects on DPPH and ·OH radicals, total reduction ability and total antioxidant ability.Results: 61 compounds were identified in D. huoshanense and 49 compounds were identified in D. officinale and with 43 shared compounds; Among them, 44 and 7 compounds were respectively identified from the two for the first time. Oxyresveratrol, quercetin-3-O-glucoside, quercetin-3-O-arabinoside, naringenin-7-O-glucoside, baicalin, hesperetin, vitexin and apigenin-6-C-pentosyl-8-C-(2’’-O-quinoyl)-glucoside were first identified in Dendrobium. In addition, 5 compounds were unique in D. huoshanense compared with other species of Dendrobium . The results of in vitro antioxidant activity showed that n-BuOH-soluble fraction of D. huoshanense and D. officinale both revealed the strongest antioxidant activities compared with other fractions, furthermore, the antioxidant activities of different fractions of D. huoshanense were separately superior to that of D. officinale.Conclusion: These results may provide a reference for the substances study about D. huoshanense's high-quality efficacy, and also provide a scientific basis for its development and utilization.
Exploring the Modulatory Mechanism of Eleng Capsule in the Treatment of Endometriosis Based on Integrated Strategy of Transcriptomics Combined With Network Pharmacology
Authors: Zheng Weilin , Wang Jie , Gu Jiangyong , Wu Jiayi , Wang Tao , Huang Yangxue , Liang Xuefang , Cao Lixing 
Published: October 2020

Abstract
BackgroundEndometriosis causes severe chronic pelvic pain and infertility. Chinese medicine plays an active role in the treatment of endometriosis. ELeng Capsule(ELC) is a Chinese medicine formula used for the treatment of endometriosis for several years. The previous studies have shown that ELC inhibits endometriosis. However, the mechanisms of action of ELC have not been characterized. In this study, network pharmacology and mRNA transcriptome analysis were used to study various therapeutic targets in ELC.Methods Ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry analysis(UPLC-Q-TOF/MS) was used to identify the compounds in ELC.And network pharmacology was used to analyze the network of targets and identified compounds of ELC. Apoptosis was assessed using the terminal deoxynucleotidyl transferase (TdT) deoxyuridine 5'-triphosphate (dUTP) nick-end labeling (TUNEL) assay.The protein expression of VEGFA,VEGFB,VEGFC and α-SMA in the ectopic endometrium were identified by immunohistochemistry(IHC).The level of VEGFA,VEGFB and IL1-β in serum were used by ELISA. Further, RNA-sequencing was used to identify differentially expressed genes (DEGs) in ELC. Biological functions and pathways were determined through the Gene Ontology(GO) and Kyoto encyclopedia of genes and genomes pathway(KEGG) analyses. In addition, Gene Set Enrichment Analysis (GSEA) analysis was used to further analyze the genetic network and modular genetics. ResultsWe had identified 26 new bioactive compounds in ELeng Capsule by UPLC-Q-TOF/MS analysis. The KEGG pathways of ELC associtaed targets related to neuroactive ligand-receptor interaction, the toll-like receptor signaling pathway, and the vascular endothelial growth factor signaling pathway by network pharmacology analysis.Further, ELC could induce cell apoptosis, inhibit angiogenesis through reduce the expression of VEGFA and VEGFC, and inhibit the fibrosis through reduce the expression of α-SMA in ectopic lesions(P＜0.05). In addition, the development of endometriosis in the rat model may be related to mechanisms of inflammation,epithelial-mesenchymal transition(EMT) by RNA-sequencing. And the targets in the treatment of ELC were predominantly associated with actin and cytoskeleton,EMT,focal adhesion,and inflammatory immunity based on the DEGs analysis. In additon, the GSEA analysis showed that the treatment of ELC was associated with the Notch signaling pathway, Hippo signaling pathway, etc.ConclusionELeng Capsules exerted beneficial effects against endometriosis, potentially by induce apoptosis,modulating the angiogenesis,cytoskeleton, epithelial-mesenchymal transition(EMT), and cell junction-associated pathways,etc. These findings could provide evidence for an innovative treatment strategy and novel therapeutic targets for endometriosis.
ShenLing BaiZhu San alleviates Ulcerative Colitis in Rats by Regulating Gut Microbiota
Authors: Gu Daxing , Zhou Shanshan , Yao Lili , Tan Ying , Chi Xingzi , Shi Dayou , Guo Shining , Liu Cui 
Published: October 2020

Abstract
Background: Recent studies have suggested that Shenling Baizhu San (SLBZS), an complementary and alternative medical therapy for ulcerative colitis (UC), alleviate clinic symptoms by the improvement of biochemical criteria and restoration of the intestinal barrier function. SLBZS as a famous Chinese herbal formula has been reportedly used to treat UC, of which mechanism is unknown. This study investigated the therapeutic effects of SLBZS on restoring the gut microbiota in a UC rat model. Methods: We proposed a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced UC rat model to monitor the structural modulation of the gut microbiota. The test period was 10 days (observation for two days after modeling, treatment for 8 days by SLBZS). In this study, the level of inflammatory cytokines and activity of antioxidant enzymes in serum were ascertained by enzyme-linked immunosorbent assay (ELISA) and histological changes of colon were observed. Feces were collected for high-throughput sequencing of 16S rRNA gene. Results: SLBZS partly reduced the diversity of the gut microbiota, while the abundance of that is increased. Furthermore, at the genus level, the relative abundance of short chain fatty acids (SCFA) producing bacteria including Prevotella and Oscillospira increased, while the relative abundance of harmful bacteria including Desulfovibrio, and Bilophila decreased. Additionally, SLBZS could improve the lesions of colon and significantly reduce the expression of Interleukin-6 (IL-6) and Myeloperoxidase (MPO) and increase the activities of Superoxide dismutase (SOD) and Catalase (CAT) in rats serum. Conclusions: These results demonstrate that SLBZS may treat UC effectively by inhibiting inflammation, enhancing antioxidant capacity and regulating gut microbiota.
Exploring the Pharmacological Mechanism of Cassiae Semen a Cting on Cataracts Based on a Network Pharmacology Approach
Authors: Zhong Ying , Fang Youfa 
Published: October 2020

Abstract
BackgroundCassiae Semen (CS) is one of the most well-known herbs used in the treatment of cataracts in China. However, the potential mechanisms of its anti-cataracts effects have not been fully explored.MethodThe active compounds of CS were obtained from TCMSP database, and their targets were retrieved from the TCMSP, STITCH and DrugBank databases. Cataracts related target genes were identified from the GeneCard, Malacard, and OMIM databases. GO and KEGG analysis were performed using DAVID online tools, and Cytoscape were used to construct compound-targets network and protein-protein interaction (PPI) networks, cluster analysis were carried out using MCODE plugin for Cytoscape.ResultsWe obtained 13 active compounds from CS and 105 targets in total to construct a compound-target network, which indicated that emodin, stigmastero, and rhein served as the main ingredients in CS. A total of 238 cataracts related targets were identified from public databases. PPI networks of compound targets and cataract-related targets were constructed and merged to obtained the central network, enrichment analysis showed 50 key targets in the central network enriched in several important signaling pathways, such as thyroid hormone signaling pathway, MAPK signaling pathway, PI3K-Akt signaling pathway. The top 4 genes with higher degree in the central network were TP53, HSP90, ESR1, EGFR, indicating their important roles in the treatment of cataracts.ConclusionsThe present study systematically revealed the multi-target mechanisms of CS on cataracts using network pharmacology approach, and provided indications for further mechanistic studies and also for the development of CS as a potential treatment for cataracts patients.
Synthesis, Structural and Dielectric Studies on SrBi2-xGdxNb2O9 Lead Free Ceramics
Authors: Jaladi Nitchal Kiran , Rao K. Sambasiva , Workineh Haileeyesus , Kiran J. Anindhya , Nagamani S. 
Published: October 2020

Abstract
In this manuscript, the structural and dielectric properties of Gadolinium (Gd3+) substituted at Bi-site of SrBi2-xGdxNb2O9 (x= 0.0, 0.4, 0.6 and 0.8) prepared by using solid state reaction are studied. XRD analysis revealed the formation of single phase with orthorhombic structure in SBN and Gadolinium modified SBN. It is found that cell parameters and volume were decreased with increase of Gd3+ ion concentration in SBN. SEM analysis revealed that the samples possess well defined needle shaped grains. The grain size of SBN was hindered by the presence of Gd3+ ion at Bi-site. The growth of single phase layered perovskite structure was confirmed from FTIR and Raman spectroscopy. The dielectric properties of Gd3+ ion doped SBN ceramics are studied as a function of frequency (50Hz-1MHz) from room temperature to 500ºC. It is observed that phase transition temperature (Tc) decreased from 430ºC to 330ºC with increase of frequency due to incorporation of Gd3+ ion in SBN. The broadness of peaks and decrease in Tc indicate the transition from a normal ferroelectric to ferroelectric-relaxor type. The study on variation of tanδ with temperature at different frequencies indicates that tanδ has larger values at higher temperatures. Further, the diffuseness parameter (γ) has been computed for all the compositions.
Characteristics of confirmed coronavirus disease cases at the south campus of Shanghai East Hospital: A retrospective study
Authors: Fan Chenghui , Qu Lihong , Guo Zhongliang , Zhang Jianhua , Wang Jun , Li Qin , Kang Sheng , Wang Yue , Xu Lei , Wang Wei , Yang Wenzhong , Zhou Ying , Wang Yuanyuan , Hao Ying , Li Ruilin , Sun Feng , Xue Junli 
Published: October 2020

Abstract
Background: A fever outpatient clinic at the south campus of Shanghai East Hospital (SEH) openedin response to the coronavirus disease (COVID-19) outbreak. We analyzed the data of all 11,972patients who visited the fever clinic and the 29 confirmed COVID-19 cases to determine the clinical and epidemiological characteristics of confirmed COVID-19 cases diagnosed at SEH.Methods: Data were collected from all fever outpatient clinic patients between January 23 and September 30, 2020. We compared the characteristics of confirmed patients, including age, occupation, area, symptoms, laboratory results, and computed tomography (CT) findings, according to month.Results: By September 30, 2020, 11,972 patients, including 29 (0.24%) confirmed COVID-19 cases, visited the clinic. Four of five confirmed domestic cases identified during January–February 2020 were from Wuhan (mainly elderly retirees and local employees), Hubei. After the epidemic spread internationally, all 22 confirmed cases identified during March–April 2020 were individuals who returned from abroad. They were predominantly young Chinese international students. The sporadic two confirmed cases during May–September 2020 included an employee returning to work from Hubei and an Indian servant from abroad. Symptoms, laboratory tests, and CT findings were consistent with previous reports of COVID-19-positive cases.Conclusions: The characteristics of confirmed COVID-19 cases at SEH varied among different periods in response to the spread of the pandemic. However, due to the effective early isolation and quarantine measures, no outbreak occurred in SEH, which contributed to the prevention and control of the epidemic in Shanghai.
Effect of Levosimendan on the Prognosis of Cardiac Surgery in Patients with Cardiac Insufficiency
Authors: Na Heya , Fa Xianen , Zhou Yuyang , Li Xiangyang , Zhu Rui , Liu Lei , Liu Bing 
Published: October 2020

Abstract
Objective: To retrospectively analyze the effect of levosimendan on the survival and prognosis of cardiac surgery patients with LVEF < 40%. Methods: the clinical data of 224 patients with preoperative LVEF < 40% were retrospectively analyzed. According to different treatment schemes, the patients were divided into levosimendan group (n = 60) and no-levosimendan group (n = 164,). The control group was treated with routine treatment, and the observation group was treated with levosimendan on the basis of routine treatment. Then a multivariate logistic regression model with a propensity score analysis was used to limit biases and finally the data of 40 patients in each group were selected for analysis. Results: Hemodynamic data showed that the cardiac index, LVEF and PAOP of patients in levosimendan group were significantly improved. The concentration of serum lactic acid in the levosimendan group was lower than that in the control group (P < 0.05). At the same time, postoperative ICU and hospital stay were significantly reduced in levosimendan group (P < 0.05.), Logistics regression analysis showed that levosimendan was the only protective factor for Low cardiac output syndrome (LCOS) (HR=4.33; 95% confidence interval: 1.27- 14.78; P = 0.019). Conclusion: levosimendan can better improve hemodynamics and reduce postoperative ICU time and hospital stay. The use of it tended to decrease the incidence of LCOS significantly.