bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-nuclei RNA-sequencing of plant tissues
Authors: Sunaga-Franze Daniele Y. , Muino Jose M. , Braeuning Caroline , Xu Xiaocai , Zong Minglei , Smaczniak Cezary , Yan Wenhao , Fischer Cornelius , Vidal Ramon , Kliem Magdalena , Kaufmann Kerstin , Sauer Sascha 
Published: November 2020

SUMMARYSingle-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nuclei isolation combined with nanowell-based library preparation. Our method enables the transcriptome analysis of thousands of individual plant nuclei. It serves as alternative to the use of protoplast isolation, which is currently a standard methodology for plant single-cell genomics, although it can be challenging for some plant tissues. We show the applicability of our nuclei isolation method by using different plant materials from several species. The potential of our snRNA-seq method is shown through the characterization of transcriptomes of seedlings and developing flowers fromArabidopsis thaliana. We evaluated the transcriptome dynamics during the early stages of anther development, identify stage-specific transcription factors regulating this process and the prediction of their target genes. Our nuclei isolation procedure can be applied in different plant species and tissues, thus expanding the toolkit for plant single-cell genomics experiments.SIGNIFICANCE STATEMENTWe introduce an optimized plant nuclei isolation procedure followed by single nuclei RNA-seq that can be applied to different plant tissues without the need for protoplast isolation.
Characterization of plasma membrane proteins from maize roots (Zea mays L.) under multiple abiotic stressesusingLC-MS/MS technique
Authors: Rafique Suphia , Nasrullah Nazima 
Published: November 2020

AbstractThe aim of this study was to understand the response of maize inbred plants showing tolerance when exposed to various abiotic stresses (drought x low-N and waterlogging x low-N stress) simultaneously. The plants under stress expressed higher photosynthetic efficiency, increase in plant height, leaf area, and were able to maintain relatively high leaf relative water content and less decrease in morphological parameters. Therefore, to understand the processes controlling the tolerance to various stresses we analyzed maize roots plasma membranes proteome of treated plants only using LC-MS/MS techniques. The large number of proteins (295) were identified which were mainly trans membrane proteins, low abundance proteins, and root specific proteins. Further, a few proteins were selected like high-affinity Nitrate transporter, NR enzyme, PEP carboxylase, and Glutamine synthetase proteins their induction were validated by qRT-PCR approach in control and treated plants. The qRT-PCR results indicated the gene of all four proteins were expressed in treated and control plants. We concluded the high-affinity nitrate transporter proteins might represent the executive part of the protective response that plays a significant role in low-N stress tolerance. The presence of other major proteins like kinases, stress-responsive TFs, calmodulin, aquaporins, stress-related proteins, and many more proteins and their interaction with nitrate transporter proteins and their role can be validated only after comparing it with control samples.
Analysis of cell-type-specific chromatin modifications and gene expression inDrosophilaneurons that direct reproductive behavior
Authors: Palmateer Colleen M , Moseley Shawn C , Ray Surjyendu , Brovero Savannah G , Arbeitman Michelle N 
Published: November 2020

AbstractExamining the role of chromatin modifications and gene expression in neurons is critical for understanding how the potential for behaviors are established and maintained. We investigate this question by examiningDrosophila melanogaster fru P1neurons that underlie reproductive behaviors in both sexes. We developed a method to purify cell-type-specific chromatin (Chromatag), using a tagged histone H2B variant that is expressed using the versatile Gal4/UAS gene expression system. Here, we use Chromatag to evaluate five chromatin modifications, at three life stages in both sexes. We find substantial changes in chromatin modification profiles across development and fewer differences between males and females. We generated cell-type-specific RNA-seq data sets, using translating ribosome affinity purification (TRAP), and identify actively translated genes infru P1neurons, revealing novel stage- and sex-differences in gene expression. We compare chromatin modifications to the gene expression data and find patterns of chromatin modifications associated with gene expression. An examination of the genic features where chromatin modifications resides shows certain chromatin modifications are maintained in the same genes across development, whereas others are more dynamic, which may point to modifications important for cell fate determination in neurons. Using a computational analysis to identify super-enhancer-containing genes we discovered differences across development, and between the sexes that are cell-type-specific. A set of super-enhancer-containing genes that overlapped with those determined to be expressed with the TRAP approach were validated as expressed infru P1neurons.Author SummaryDifferences in male and female reproductive behaviors are pervasive in nature and important for species propagation. Studies of sex differences in the fruit fly,Drosophila melanogaster, have been ongoing since the early 1900s, with many of the critical molecular and neural circuit determinates that create sexually dimorphic behavior identified. This system is a powerful model to understand fundamental principles about the underpinnings of complex behavior at high resolution. In this study, we examine the gene expression and chromatin modification differences specifically in a set of neurons that direct male and female reproductive behaviors inDrosophila. We describe differences across development and between the sexes with the goal of understanding how the potential for behavior is created and maintained.
Innovative entrepreneurship and quality of life. The case of Ecuador
Authors: Aguirre Juan Carlos , Peralta Zuñiga Marco Leonardo , Mora Pedro , Blanco Francisco 
Published: November 2020

PurposeThis article is based on the assumption that entrepreneurship improves quality of life (HDI). Its main objective is to establish causal relationships between entrepreneurship variables such as credits, innovation (R&D), business growth, foreign direct investment and the Global Competitiveness Index and how these have influenced a country's development.Design/methodology/approachTo analyse and validate this assumption, relevant information has been extracted about Ecuador (the subject of the study) for the 1998–2017 period. The information has received the respective econometric treatment, through a multivariate estimation by the autoregressive vector (ARV) method that made it possible to establish impulse-response functions.ResultsThe results indicate that there is a significant and positive statistical impact between the variables related to entrepreneurship and quality of life (HDI), with the exception of “Innovation”, which is not representative in the model, demonstrating that the investment made at country level in R&D is not sufficient to have an impact on the HDI. It was also determined that promoting entrepreneurship would be useful as this would alter the trend of the variables, making them conducive to increasing the HDI.Originality/valueThis article is one of the few to address this issue. It includes the self-regressive vector model as a key methodology used to evaluate and establish public policies. RVM has provided positive results in the field of economics and can be adopted in the area of entrepreneurship.
Customer effort in mandatory and voluntary value cocreation: a study in a health care context
Authors: Pham Tram-Anh Ngoc , Sweeney Jillian Carol , Soutar Geoffrey Norma 
Published: November 2020

Purpose
This study aims to examine the impacts various types of resources had on customer effort in mandatory and voluntary value cocreation activities and the contribution of efforts in these different activity types to quality of life.

Design/methodology/approach
Data from customers across five chronic health conditions were collected through an online survey. Rasch analysis helped identify hierarchies of activities representing varying levels of effort across four activity types (mandatory (customer), mandatory (customer or organization), voluntary in-role and voluntary extra-role activities). The conceptual model that was developed to examine the relationships of interest was analyzed using partial least squares structural equation modeling.

Findings
While clinical resources helped mandatory activities and personal network resources facilitated voluntary activities, psychological resources had greater impacts on customer effort across the whole range of activities. Effort in each activity type contributed to the quality of life differently, with voluntary activities having the greatest impacts on quality of life.

Practical implications
This study lends support to a holistic approach to health service that requires the mobilization of networks of resources to encourage customers’ engagement in a broad range of activities. Understanding the resources facilitating effort in distinct activity types provides insights to develop strategies to drive value cocreation efforts that subsequently contribute to improvements in quality of life.

Originality/value
Drawing on an extensive and nuanced categorization of activities, this study broadened the understanding of the networks of resources that are integrated in customer value cocreation processes and the link between value cocreation efforts and quality of life.
The effectiveness of artificial neural networks applied to analytical procedures using high level data: a simulation analysis
Authors: Li Stewart , Fisher Richard , Falta Michael 
Published: November 2020

Purpose
Auditors are required to perform analytical procedures during the planning and concluding phases of the audit. Such procedures typically use data aggregated at a high level. The authors investigate whether artificial neural networks, a more sophisticated technique for analytical review than typically used by auditors, may be effective when using high level data.

Design/methodology/approach
Data from companies operating in the dairy industry were used to train an artificial neural network. Data with and without material seeded errors were used to test alternative techniques.

Findings
Results suggest that the artificial neural network approach was not significantly more effective (taking into account both Type I and II errors) than traditional ratio and regression analysis, and none of the three approaches provided more overall effectiveness than a purely random procedure. However, the artificial neural network approach did yield considerably fewer Type II errors than the other methods, which suggests artificial neural networks could be a candidate to improve the performance of analytical procedures in circumstances where Type II error rates are the primary concern of the auditor.

Originality/value
The authors extend the work of Coakley and Brown (1983) by investigating the application of artificial neural networks as an analytical procedure using aggregated data. Furthermore, the authors examine multiple companies from one industry and supplement financial information with both exogenous industry and macro-economic data.
Entrepreneurship via social networks – “connected woman” in Lebanon
Authors: Lichy Jessica , Farquhar Jillian Dawes , Kachour Maher 
Published: November 2020

Purpose
The purpose of this paper is to extend understanding of marketing in MENA by investigating how women entrepreneurs use social networking sites (SNS) in marketing their businesses in Lebanon.

Design/methodology/approach
To address contextual issues arising from research in this region, this study consists of a two-phase research design of, first, a panel of specialised business commentators and, second, digital qualitative data collection that enabled access to hard to reach informants.

Findings
The study reveals that the activities of women entrepreneurs are fundamentally enabled by SNS as it allows them to optimise their networks in prospecting, communicating and developing relationships with stakeholders. It also allows them to support the social fabric of the family unit by providing an extra source of income and facilitating connections.

Research limitations/implications
This study draws on a single country within the MENA region; nonetheless, the analysis offers new and nuanced understanding to marketing of small businesses in uncovering how Lebanese women entrepreneurs are able to build and run their businesses using SNS.

Practical implications
This research demonstrates how women entrepreneurs can set up and run businesses using SNS to reach and extend their networks in a culturally diverse and growing economy. SNS provides an inclusive platform through which women build and run a small business.

Social implications
This research responds to a World Development aim of studying the relationships between gender and trade such as women entrepreneurs using social technologies.

Originality/value
This research responds to a World Development aim of studying the relationships between gender and trade, here by investigating how women entrepreneurs set up and run small businesses enabled by SNS.
The relationship between managers' narcissism and overconfidence on corporate risk-taking
Authors: Salehi Mahdi , Afzal Aghaei Naeini Amirhosein , Rouhi Safoura 
Published: November 2020

PurposeThe primary purpose is to investigate the relationship between narcissism and managers' overconfidence in listed companies' risk-taking.Design/methodology/approachIn this study, two criteria of signature and reward are used to measure manager's narcissism; manager's overconfidence, using multiple regression models and finally to measure companies' risk-taking by using companies' monthly returns. Multiple regression is employed to test the model using a sample of 890 firm-year participation on the Tehran Stock Exchange from 2012 to 2017 with panel data and model with fixed effects.FindingsThe findings indicate that the CEO's narcissism and the board of directors positively and significantly affect corporate risk-taking. Also, managers' overconfidence has a positive and significant relationship with corporate risk-taking.Originality/valueThe results of this study identified other factors affecting companies' risk-taking. This study also contributed to the development of the literature on narcissism, overconfidence and corporate risk-taking.
Monoclonal antibodies against foot‐and‐mouth disease virus RNA polymerase for detection of virus infection
Authors: Kushwaha Anand , Behera Subhasmita , Saminathan Mani , Sreedharan Krishnan , Basagoudanavar Suresh , Manjunatha Reddy G. B. , Hosamani Madhusudan 
Published: November 2020

AbstractFoot‐and‐mouth disease (FMD) is a major viral disease in farm animals. In the present study, seven monoclonal antibodies (mAbs) were produced against the FMD virus (FMDV)‐encoded RNA‐dependent RNA polymerase (3D protein) and characterized. Screening of mAb reactivity against three overlapping fragments of the 3D protein expressed in Escherichia coli revealed that the binding sites of all the mAbs were confined to the N‐terminal one‐third of the 3D protein. A selected mAb was utilized for detecting FMDV in the infected cell culture and tissues obtained from FMDV‐infected animals.
Geographical personality gradient in herbivorous animals: Implications for selective culling to reduce crop damage
Authors: Honda Takeshi 
Published: November 2020

AbstractTraditional lethal techniques used to mitigate wildlife damage have targeted entire populations through the use of random harvesting. However, recent literature has suggested that controlling population size by random harvesting is not the only mean to mitigate human‐wildlife conflicts. Controlling problem individuals may also be important because individuals with behavioral characteristics such as greater boldness and low neophobia are predicted to cause more damage to crops. Reducing the number of bold individuals may therefore mitigate crop damage, but a method to selectively cull bold individuals has not been determined. Hence, this study aimed to demonstrate their distribution in native habitats because if bold and shy individuals prefer different habitats, selective culling could target areas preferred by bold individuals. To detect differences in neophobia (i.e., shyness/boldness), 17 visible flash and invisible infrared flash sensor cameras were randomly placed in forest areas based on the assumption that neophobic (shy) sika deer (Cervus nippon) would be deterred by visible flash cameras (a novel stimulus). The relationship between camera flash type and the probability of detection (i.e., of being photographed by a camera) was used as an index of shyness/boldness. Geographical information system and regression analyses showed that bold individuals preferred forested areas near farmland. These results indicate that selective lethal control could be conducted in forest areas near farmlands to mitigate crop damage.
The landscapes of minus. Hatred, adolescence and the paradoxes of growth
Authors: Bisagni Francesco 
Published: November 2020

AbstractThis paper explores Bion’s theory of links, L, H, K (Love, Hate and Knowledge) and their minus counterparts, ‐L, ‐H, ‐K, which are not conceivable as simply opposite to or as a lack of L, H, K. Rather, they correspond to a way of experiencing Love, Hatred and Knowledge in terms of absoluteness, and in terms of a radical impossibility of acknowledging loss, relativeness and absence. The theory of links is also examined in its evolution towards the conceptualization of three types of container/contained configurations (commensal, symbiotic and parasitic). These Bionian models are compared and referred to the way Jung articulates the coexistence of ‘good’ and ‘evil’, and the paradoxical nature of mental functioning in relation to the individuation process. The images taken from the Rosarium Philosophorum, particularly the Fons Mercurialis, examined by Jung in ‘The psychology of the transference’ (1946), are explored in the paper. Theory is examined with a particular focus on the adolescent mind and its dramatic phenomenology. Two excerpts taken from the analytic work with a mid‐adolescent female patient and a late‐adolescent male patient are presented to describe minus‐Hate as a form of absolute love, and minus‐Love as a form of absolute hatred.
Moving towards Soul – an alchemical journey. Rehabilitation of women suffering from ‘chronic pain’ in a clinical and Jungian context
Authors: Sjöström Charlotte 
Published: November 2020

AbstractThe aim of this paper is to explore the connection between symptom and symbol in the body of women suffering from chronic pain, diagnosed as fibromyalgia. The working hypothesis has been that the symbol that emerges from the symptom in the body can bridge the gap to a deeper meaning of pain and suffering, thereby becoming the agent of change for healing of the bodymind and the experience of pain in the physical body. To explore this subject I will introduce some recent research from the field of fibromyalgia, and the concepts of agency and affect systems in the body, which are important cornerstones in my work. I will briefly present my clinical concept of ‘Form and Freedom’. From this theoretical base I give some clinical examples of what I see as an alchemical journey towards soul, presented through vignettes, images and the words of three women – Maria, Riba and Ishtar. I conclude with how I see analytical psychology taking its rightful place alongside, informing or in conjunction with, as in my case, other psychotherapeutic modalities, working in creative ways that enhance healing in patients who suffer from chronic pain.
The glow of Telesphoros: a brief enquiry into the sense of the term ‘mana personality’ and the dynamic of experiences behind it
Authors: Lemos Pan 
Published: November 2020

AbstractIn this article, I begin by outlining the qualities and attributes associated with experiences of mana, focusing particularly on the way the term ‘mana’ was used by C.G. Jung in his published work. I note that Jung associated mana with the accumulation and transfer of psychic energy (libido), with the appearance and appropriation of elemental fire, and by extension, with the metamorphic experience of constellating consciousness. In this context, informed by Jung’s accounts and modes of expression, the term mana personality becomes a provisional designation for a recurrent dynamic of archetypal experiential configurations whose telos appears to be the reorientation of consciousness to its origin.
Detecting grave sites from surface anomalies: A longitudinal study in an Australian woodland
Authors: Watson Christopher J. , Ueland Maiken , Schotsmans Eline M. J. , Sterenberg Jon , Forbes Shari L. , Blau Soren 
Published: November 2020

AbstractForensic investigations of single and mass graves often use surface anomalies, including changes to soil and vegetation conditions, to identify potential grave locations. Though numerous resources describe surface anomalies in grave detection, few studies formally investigate the rate at which the surface anomalies return to a natural state; hence, the period the grave is detectable to observers. Understanding these processes can provide guidance as to when ground searches will be an effective strategy for locating graves. We studied three experimental graves and control plots in woodland at the Australian Facility for Taphonomic Experimental Research (Sydney, Australia) to monitor the rate at which surface anomalies change following disturbance. After three years, vegetation cover on all grave sites and control plots had steadily increased but remained substantially less than undisturbed surroundings. Soil anomalies (depressions and cracking) were more pronounced at larger grave sites versus the smaller grave and controls, with leaf litterfall rendering smaller graves difficult to detect beyond 20 months. Similar results were observed in two concurrent burial studies, except where accelerated revegetation appeared to be influenced by mummified remains. Extreme weather events such as heatwaves and heavy rainfall may prolong the detection window for grave sites by hindering vegetation establishment. Observation of grave‐indicator vegetation, which exhibited abnormally strong growth 10 months after commencement, suggests that different surface anomalies may have different detection windows. Our findings are environment‐specific, but the concepts are applicable globally.