bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recharacterizing the Metabolic State of Energy Balance in Thrifty and Spendthrift Phenotypes
Authors: Hollstein Tim , Basolo Alessio , Ando Takafumi , Votruba Susanne B , Walter Mary , Krakoff Jonathan , Piaggi Paolo 
Published: March 2020

AbstractPurposeThe human thrifty phenotype hypothesis presupposes that lower 24-hour (24h) energy expenditure (24EE) during famine preserves body mass and promotes survival. The prevailing view defines thrifty individuals as having a lower 24EE during fasting. However, it is also plausible that the greater decline in 24EE during fasting in thrifty individuals is due to higher 24EE during energy balance conditions (ENBAL). Herein, we provide evidence that this is indeed the case.MethodsIn 108 healthy subjects, 24EE was measured in a whole-room indirect calorimeter both during ENBAL and 24h fasting conditions. Subjects were categorized as thrifty or spendthrift based on the median value (−162 kcal/day) of the difference in 24EE (adjusted for body composition) between fasting and ENBAL conditions. Concomitant 24h urinary catecholamines were assessed by liquid chromatography–mass spectrometry.ResultsCompared to ENBAL, 24EE decreased during 24h fasting by 172 kcal/day (standard deviation = 93; range, −470 to 122). A greater-than-median decrease in 24EE (“thriftier” phenotype) was due to higher 24EE during ENBAL (+124 kcal/day; P &lt; 0.0001) but not to lower 24EE during fasting (P = 0.35). Greater fasting-induced increase in epinephrine was associated with concomitant lower decrease in 24EE (r = 0.27; P = 0.006).Main ConclusionThe greater decrease in 24EE during acute fasting (which characterizes the thrifty phenotype) is not due to reduced metabolic rate during fasting but to a relatively higher 24EE during feeding conditions, and this decrease in 24EE during fasting is accompanied by a smaller increase in epinephrine. These results recharacterize the prevailing view of the short-term 24EE responses that define the human metabolic phenotypes.Clinical Trials: NCT00523627, NCT00687115, NCT02939404
Integration of luminal pressure and signalling in tissue self-organization
Authors: Chan Chii J. , Hiiragi Takashi 
Published: March 2020

ABSTRACT
Many developmental processes involve the emergence of intercellular fluid-filled lumina. This process of luminogenesis results in a build up of hydrostatic pressure and signalling molecules in the lumen. However, the potential roles of lumina in cellular functions, tissue morphogenesis and patterning have yet to be fully explored. In this Review, we discuss recent findings that describe how pressurized fluid expansion can provide both mechanical and biochemical cues to influence cell proliferation, migration and differentiation. We also review emerging techniques that allow for precise quantification of fluid pressure in vivo and in situ. Finally, we discuss the intricate interplay between luminogenesis, tissue mechanics and signalling, which provide a new dimension for understanding the principles governing tissue self-organization in embryonic development.
Single-cyst transcriptome analysis of Drosophila male germline stem cell lineage
Authors: Shi Zhen , Lim Cindy , Tran Vuong , Cui Kairong , Zhao Keji , Chen Xin 
Published: March 2020

ABSTRACT
The Drosophila male germline stem cell (GSC) lineage provides a great model to understand stem cell maintenance, proliferation, differentiation and dedifferentiation. Here, we use the Drosophila GSC lineage to systematically analyze the transcriptome of discrete but continuously differentiating germline cysts. We first isolated single cysts at each recognizable stage from wild-type testes, which were subsequently applied for RNA-seq analyses. Our data delineate a high-resolution transcriptome atlas in the entire male GSC lineage: the most dramatic switch occurs from early to late spermatocyte, followed by the change from the mitotic spermatogonia to early meiotic spermatocyte. By contrast, the transit-amplifying spermatogonia cysts display similar transcriptomes, suggesting common molecular features among these stages, which may underlie their similar behavior during both differentiation and dedifferentiation processes. Finally, distinct differentiating germ cell cyst samples do not exhibit obvious dosage compensation of X-chromosomal genes, even considering the paucity of X-chromosomal gene expression during meiosis, which is different from somatic cells. Together, our single cyst-resolution, genome-wide transcriptional profile analyses provide an unprecedented resource to understand many questions in both germ cell biology and stem cell biology fields.
Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence
Authors: Roychoudhury Kaushik , Salomone Joseph , Qin Shenyue , Cain Brittany , Adam Mike , Potter S. Steven , Nakafuku Masato , Gebelein Brian , Campbell Kenneth 
Published: March 2020

ABSTRACT
The Gsx2 homeodomain transcription factor promotes neural progenitor identity in the lateral ganglionic eminence (LGE), despite upregulating the neurogenic factor Ascl1. How this balance in maturation is maintained is unclear. Here, we show that Gsx2 and Ascl1 are co-expressed in subapical progenitors that have unique transcriptional signatures in LGE ventricular zone (VZ) cells. Moreover, whereas Ascl1 misexpression promotes neurogenesis in dorsal telencephalic progenitors, the co-expression of Gsx2 with Ascl1 inhibits neurogenesis. Using luciferase assays, we found that Gsx2 reduces the ability of Ascl1 to activate gene expression in a dose-dependent and DNA binding-independent manner. Furthermore, Gsx2 physically interacts with the basic helix-loop-helix (bHLH) domain of Ascl1, and DNA-binding assays demonstrated that this interaction interferes with the ability of Ascl1 to bind DNA. Finally, we modified a proximity ligation assay for tissue sections and found that Ascl1-Gsx2 interactions are enriched within LGE VZ progenitors, whereas Ascl1-Tcf3 (E-protein) interactions predominate in the subventricular zone. Thus, Gsx2 contributes to the balance between progenitor maintenance and neurogenesis by physically interacting with Ascl1, interfering with its DNA binding and limiting neurogenesis within LGE progenitors.
Plakophilin 3 phosphorylation by ribosomal S6 kinases supports desmosome assembly
Authors: Müller Lisa , Rietscher Katrin , Keil René , Neuholz Marvin , Hatzfeld Mechthild 
Published: March 2020

ABSTRACT
Desmosome remodeling is crucial for epidermal regeneration, differentiation and wound healing. It is mediated by adapting the composition, and by post-translational modifications, of constituent proteins. We have previously demonstrated in mouse suprabasal keratinocytes that plakophilin (PKP) 1 mediates strong adhesion, which is negatively regulated by insulin-like growth factor 1 (IGF1) signaling. The importance of PKP3 for epidermal adhesion is incompletely understood. Here, we identify a major role of epidermal growth factor (EGF), but not IGF1, signaling in PKP3 recruitment to the plasma membrane to facilitate desmosome assembly. We find that ribosomal S6 kinases (RSKs) associate with and phosphorylate PKP3, which promotes PKP3 association with desmosomes downstream of the EGF receptor. Knockdown of RSKs as well as mutation of an RSK phosphorylation site in PKP3 interfered with desmosome formation, maturation and adhesion. Our findings implicate a coordinate action of distinct growth factors in the control of adhesive properties of desmosomes through modulation of PKPs in a context-dependent manner.
Current solutions for the treatment of chronic articular cartilage defects in the knee
Authors: Chimutengwende-Gordon Mukai , Donaldson James , Bentley George 
Published: March 2020

Chondral and osteochondral defects in the knee are common and may lead to degenerative joint disease if treated inappropriately. Conventional treatments such as microfracture often result in fibrocartilage formation and are associated with inferior results. Additionally, microfracture is generally unsuitable for the treatment of defects larger than 2–4 cm2. The osteochondral autograft transfer system (OATS) has been shown to produce superior clinical outcomes to microfracture but is technically difficult and may be associated with donor-site morbidity. Osteochondral allograft use is limited by graft availability and failure of cartilage incorporation is an issue. Autologous chondrocyte implantation (ACI) has been shown to result in repair with hyaline-like cartilage but involves a two-stage procedure and is relatively expensive. Rehabilitation after ACI takes 12 months, which is inconvenient and not feasible for athletic patients. Newer methods to regenerate cartilage include autologous stem cell transplantation, which may be performed as a single-stage procedure, can have a shorter rehabilitation period and is less expensive than ACI. Longer-term studies of these methods are needed.Cite this article: EFORT Open Rev 2020;5:156-163. DOI: 10.1302/2058-5241.5.190031
High-speed SSB transmission with a silicon MZM and a soft combined artificial neural network-based equalization
Authors: Ming Hao , Zhang Lei , Yang Fan , Ruan Xiaoke , Zhang Fan 
Published: March 2020

We experimentally demonstrate high-speed metro-scale optical transmission of a single sideband (SSB) 4-ary pulse amplitude modulation (PAM-4) signal based on a silicon photonic dual-drive Mach–Zehnder modulator (MZM). We propose a novel, to the best of our knowledge, artificial neural network (ANN) structure of soft combined ANN (SC-ANN) to compensate for both linear and nonlinear impairments of the signal. SC-ANN obtains the enhanced performance by averaging the outputs of conventional ANN with different sizes. With the help of the SC-ANN, we achieve a 320 km standard single-mode fiber (SSMF) transmission of 184 Gb/s (92Gbaud) PAM-4 with a bit-error rate (BER) below the 20% soft-decision forward error-correction (SD-FEC) threshold of

2.4

×

10

−
2

, and the optical signal-to-noise ratio (OSNR) penalty is only 0.3 dB compared to the back-to-back (BTB) results.
Kilowatt-level ytterbium-Raman fiber amplifier with a narrow-linewidth and near-diffraction-limited beam quality
Authors: Ma Pengfei , Miao Yu , Liu Wei , Meng Daren , Zhou Pu 
Published: March 2020

By focusing on a typical emitting wavelength of 1120 nm as an example, we present the first, to the best of our knowledge, demonstration of a high-efficiency, narrow-linewidth kilowatt-level all-fiber amplifier based on hybrid ytterbium-Raman (Yb-Raman) gains. Notably, two temporally stable, phase-modulated single-frequency lasers operating at 1064 nm and 1120 nm, respectively, were applied in the fiber amplifier, to simultaneously alleviate the spectral broadening of the 1120 nm signal laser and suppress the stimulated Brillouin scattering effect. An over 1 kW narrow-linewidth 1120 nm signal laser was obtained with slope efficiency of

∼

77

%

and beam quality of

M

x
2

∼

1.25

,

M

y
2

∼

1.17

. The amplified spontaneous emission (ASE) noise in the fiber amplifier was effectively suppressed by incorporating an ASE-filtering system between the seed laser and the main amplifier. Furthermore, the experimental results demonstrate that the spectral linewidth broadening effect is tightly related to the injected power ratios between the two seed lasers. Overall, this setup could provide a reference on obtaining and optimizing high-power narrow-linewidth fiber lasers operating in the long wavelength extreme of the Yb gain spectrum.
Femtometer-resolved simultaneous measurement of multiple laser wavelengths in a speckle wavemeter
Authors: Bruce Graham D. , O’Donnell Laura , Chen Mingzhou , Facchin Morgan , Dholakia Kishan 
Published: March 2020

Many areas of optical science require an accurate measurement of optical spectra. Devices based on laser speckle promise compact wavelength measurement, with attometer-level sensitivity demonstrated for single wavelength laser fields. The measurement of multimode spectra using this approach would be attractive, yet this is currently limited to picometer resolution. Here, we present a method to improve the resolution and precision of speckle-based multi-wavelength measurements. We measure multiple wavelengths simultaneously, in a device comprising a single 1-m-long step-index multimode fiber and a fast camera. Independent wavelengths separated by as little as 1 fm are retrieved with 0.2 fm precision using principal component analysis. The method offers a viable way to measure sparse spectra containing multiple individual lines and may find application in the tracking of multiple lasers in fields such as quantum technologies and optical telecommunications.
High pulse energy and quantum efficiency mid-infrared gas Raman fiber laser targeting CO2 absorption at 4.2  µm
Authors: Wang Yazhou , Dasa Manoj K. , Adamu Abubakar I. , Antonio-Lopez J. E. , Habib Md. Selim , Amezcua-Correa Rodrigo , Bang Ole , Markos Christos 
Published: March 2020

In this Letter, we demonstrate a high pulse energy and linearly polarized mid-infrared Raman fiber laser targeting the strongest absorption line of

C
O

2

at

∼

4.2

µ

m

. This laser was generated from a hydrogen (

H

2

)-filled antiresonant hollow-core fiber, pumped by a custom-made 1532.8 nm Er-doped fiber laser delivering 6.9 ns pulses and 11.6 kW peak power. A quantum efficiency as high as 74% was achieved, to yield 17.6 µJ pulse energy at 4.22 µm. Less than 20 bar

H

2

pressure was required to maximize the pulse energy since the transient Raman regime was efficiently suppressed by the long pump pulses.
280-km experimental demonstration of a quantum digital signature with one decoy state
Authors: Ding Hua-Jian , Chen Jing-Jing , Ji Liang , Zhou Xing-Yu , Zhang Chun-Hui , Zhang Chun-Mei , Wang Qin 
Published: March 2020

A quantum digital signature (QDS) guarantees the unforgeability, nonrepudiation, and transferability of signature messages with information-theoretic security, and hence has attracted much attention recently. However, most previous implementations of QDS showed relatively low signature rates and/or short transmission distance. In this Letter, we report a proof-of-principle phase-encoding QDS demonstration using only one decoy state. First, such a method avoids the modulation of the vacuum state, thus reducing experimental complexity and random number consumption. Moreover, incorporated with low-loss asymmetric Mach–Zehnder interferometers and a real-time polarization calibration technique, we have successfully achieved a higher signature rate, e.g., 0.98 bit/s at 103 km, and to date, a record-breaking, to the best of our knowledge, transmission distance of over 280-km installed fibers. Our work represents a significant step towards real-world applications of QDS.
CD4+ regulatory T cells and CD4+ activated T cells in new active and relapse tuberculosis
Authors: Ndishimye Pacifique , Zakham Fathiah , Musanabaganwa Clarisse , Migambi Patrick , Mihai Cenariu , Soritau Olga , El Mzibri Mohammed , Pop Carmen Monica , Mutesa Leon 
Published: March 2020

The aim of the present study was to examine characteristics of tuberculosis (TB) patients with different clinical forms and to study the frequency of Regulatory T cells (Treg cells) and Activated T cells in patients with new active and relapse TB. Forty-five pulmonary TB patients and a control group of 15 healthy individuals were enrolled in this study. Of the 45 TB patients, 15 were new cases with drug-susceptible active TB and 30 were relapsed cases (15 drug-susceptible and 15 multidrug resistant-TB). The age of study participants ranged from 21 to 68 years old. According to sex presentation, males were appreciably highly affected than females with a sex ratio of 2. The patients reported a mean recent weight loss of 8.9 kg. The Erythrocyte Sedimentation Rate was high in TB group, far exceeding the normal value. The results revealed that the number of CD3+ CD4+ T-cells significantly decreased whereas the level of blood Treg cells and expression of activation markers CD38 and HLA-DR on CD4+ T-cells significantly increased in TB group compared with the control group (p<0.05). The frequency of Treg cells was significantly higher in the TB group than the control group. Both the patients with new active TB and relapse TB demonstrated significantly higher levels of CD4+FoxP3+ Treg compared to healthy subjects (p<0.05). A high and significant percentage of Treg cells were found in patients with DS active TB than patients with MDR relapse TB. Interestingly, the frequency of CD4+FoxP3+ cells also differs according to the sputum smear microscopy status. The presence of high numbers of Treg cells and corresponding high immune activation may be an unfavourable factor that can predispose individuals to different clinical forms of TB, including relapse TB.
Familiarity Detection and Memory Consolidation in Cortical Assemblies
Authors: Zhang Xiaoyu , Yeh Fang-Chin , Ju Han , Jiang Yuheng , Quan Gabriel Foo Wei , VanDongen Antonius M.J. 
Published: March 2020

AbstractHumans have a large capacity of recognition memory (Dudai, 1997), a fundamental property of higher-order brain functions such as abstraction and generalization (Vogt and Magnussen, 2007). Familiarity is the first step towards recognition memory. We have previously demonstrated using unsupervised neural network simulations that familiarity detection of complex patterns emerges in generic cortical microcircuits with bidirectional synaptic plasticity. It is therefore meaningful to conduct similar experiments on biological neuronal networks to validate these results. Studies of learning and memory in dissociated rodent neuronal cultures remain inconclusive to date. Synchronized network bursts (SNBs) that occur spontaneously and periodically have been speculated to be an intervening factor. By optogenetically stimulating cultured cortical networks with random dot movies (RDMs), we were able to reduce the occurrence of SNBs, after which an ability for familiarity detection emerged: previously seen patterns elicited higher firing rates than novel ones. Differences in firing rate were distributed over the entire network, suggesting that familiarity detection is a system level property. We also studied the change in SNB patterns following familiarity encoding. Support vector machine (SVM) classification results indicate that SNBs may be facilitating memory consolidation of the learned pattern. In addition, using a novel network connectivity probing method, we were able to trace the change in synaptic efficacy induced by familiarity encoding, providing insights on the long-term impact of having SNBs in the cultures.
Structural Remodeling of Active Zones Is Associated with Synaptic Homeostasis
Authors: Hong Huilin , Zhao Kai , Huang Shiyan , Huang Sheng , Yao Aiyu , Jiang Yuqiang , Sigrist Stephan , Zhao Lu , Zhang Yong Q. 
Published: March 2020

Perturbations to postsynaptic glutamate receptors (GluRs) trigger retrograde signaling to precisely increase presynaptic neurotransmitter release, maintaining stable levels of synaptic strength, a process referred to as homeostatic regulation. However, the structural change of homeostatic regulation remains poorly defined. At wild-typeDrosophilaneuromuscular junction synapse, there is one Bruchpilot (Brp) ring detected by superresolution microscopy at active zones (AZs). In the present study, we report multiple Brp rings (i.e., multiple T-bars seen by electron microscopy) at AZs of both male and female larvae when GluRs are reduced. AtGluRIIC-deficient neuromuscular junctions, quantal size was reduced but quantal content was increased, indicative of homeostatic presynaptic potentiation. Consistently, multiple Brp rings at AZs were observed in the two classic synaptic homeostasis models (i.e.,GluRIIAmutant and pharmacological blockade of GluRIIA activity). Furthermore, postsynaptic overexpression of the cell adhesion protein Neuroligin 1 partially rescued multiple Brp rings phenotype. Our study thus supports that the formation of multiple Brp rings at AZs might be a structural basis for synaptic homeostasis.SIGNIFICANCE STATEMENTSynaptic homeostasis is a conserved fundamental mechanism to maintain efficient neurotransmission of neural networks. Active zones (AZs) are characterized by an electron-dense cytomatrix, which is largely composed of Bruchpilot (Brp) at theDrosophilaneuromuscular junction synapses. It is not clear how the structure of AZs changes during homeostatic regulation. To address this question, we examined the structure of AZs by superresolution microscopy and electron microscopy during homeostatic regulation. Our results reveal multiple Brp rings at AZs of glutamate receptor-deficient neuromuscular junction synapses compared with single Brp ring at AZs in wild type (WT). We further show that Neuroligin 1-mediated retrograde signaling regulates multiple Brp ring formation at glutamate receptor-deficient synapses. This study thus reveals a regulatory mechanism for synaptic homeostasis.
Pembelajaran IPA Terpadu dengan Tipe Connected dengan Model Experiential Learning untuk Meningkatkan Keterampilan Proses Sains Siswa SMP
Authors: Ridyah Surya Warni , Sriyati Siti 
Published: March 2020

AbstractThis study aims to identify the increasing mastery of skills of junior high school science students after the application of the Experiential Learning in Integrated Science learning. The method used was pre-experimental with design pretest-posttest of one-group which was conducted in one of the Junior High School in Bandung, with a sample of eighth grade students (Class VIII H) as many as 34 students in the 2nd half of 2014/2015 academic year. The research instrument was like pretest and posttest to measure the increase of science process skills, finished learning observation sheets and questionnaires to determine the response of students to the implementation of Experiential Learning model. The results showed integrated science learning by implementation of Integrated Experiential Learning model can improve skills of science process skills of junior high school students. Increased each KPS's indicator has a value that varies in sequence from largest to smallest: hypothesizing (0,65), interpretating (0,58), applying the concept (0,36) and communicating (0,35).These results indicated the model of Experiential Learning is one of learning model that can improve students' mastery KPS.Keywords: connected; experiential learning; science process skillsAbstrakPenelitian ini bertujuan untuk mengidentifikasi peningkatan keterampilan proses sains siswa SMP setelah penerapan model experiential learning pada pembelajaran IPA Terpadu. Metode penelitian yang digunakan adalah pre-eksperimen dengan desain one-group pretest-posttest yang dilakukan di salah satu SMP Negeri di Kota Bandung, dengan sampel siswa kelas VIII H sebanyak 34 siswa semester 2 Tahun Ajaran 2014/2015. Instrumen penelitian berupa soal pretest dan posttest untuk mengukur peningkatan keterampilan proses sains, lembar observasi untuk keterlaksanaan pembelajaran, dan lembar angket untuk mengetahui tanggapan siswa terhadap pelaksanaan. Hasil penelitian menunjukkan pembelajaran IPA Terpadu dengan penerapan model experiential learning dapat meningkatkan keterampilan proses sains siswa SMP pada topik tekanan. Hal ini dapat dilihat dari rata-rata skor N-Gain dari penguasaan konsep siswa sebesar 0,55 berada pada kategori sedang. Rata-rata N-gain KPS adalah 0,53 pada kategori sedang. Peningkatan masing-masing indikator KPS memiliki nilai yang bervariasi secara berurut dari yang terbesar hingga terkecil yaitu: berhipotesis (0,65), interpretasi (0,58), menerapkan konsep (0,36) dan mengkomunikasikan (0,35). Hasil-hasil ini menunjukkan model experiential learning merupakan salah satu model pembelajaran yang dapat meningkatkan keterampilan proses sains siswa.Kata Kunci: connected; experiential learning; keterampilan proses sains Permalink/DOI: http://dx.doi.org/10.15408/es.v8i2.1802