bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alpha-enolase in viral target cells suppresses the human immunodeficiency virus type 1 integration.
Authors: Kishimoto Naoki , Yamamoto Kengo , Iga Nozomi , Kirihara Chie , Takamune Nobutoki , Misumi Shogo 
Published: November 2019

Abstract
Background A protein exhibiting more than one biochemical function is termed a moonlighting protein. Glycolytic enzymes are typical moonlighting proteins, and these enzymes control the infection of various viruses. Previously, we reported that glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and alpha-enolase (ENO1) are incorporated into human immunodeficiency virus type 1 (HIV-1) particles from viral producer cells and suppress viral reverse transcription independently each other. However, it remains unclear whether these proteins expressed in viral target cells affect the early phase of HIV-1 replication. Results Here we show that the GAPDH expression level in viral target cells does not affect the early phase of HIV-1 replication, but ENO1 has a capacity to suppress viral integration in viral target cells. In contrast to GAPDH, suppression of ENO1 expression by RNA interference in the target cells increased viral infectivity, but had no effect on the expression levels of the HIV-1 receptors CD4, CCR5 and CXCR4 and on the level of HIV-1 entry. Quantitative analysis of HIV-1 reverse transcription products showed that the number of copies of the late products (R/gag) and two-long-terminal-repeat circular forms of viral cDNAs did not change but that of the integrated (Alu-gag) form increased. In contrast, overexpression of ENO1 in viral target cells decreased viral infectivity owing to the low viral integration efficiency. Results of subcellular fractionation experiments suggest that the HIV integration at the nucleus was negatively regulated by ENO1 localized in the nucleus. In addition, the overexpression of ENO1 in both viral producer cells and target cells most markedly suppressed the viral replication. Conclusions These results indicate that ENO1 in the viral target cells prevents HIV-1 integration. Importantly, ENO1, but not GAPDH, has the bifunctional inhibitory activity against HIV-1 replication. The results provide and new insights into the function of ENO1 as a moonlighting protein in HIV-1 infection.
Transcriptomic basis for salt tolerance and disease resistance of silverleaf sunflower revealed by Iso-seq and RNA-seq
Authors: Bing Jing , Ling Yunhe , An Peipei , Xiao Enshi , Li Chunlian , Song Bo , Wang Zhonghua 
Published: November 2019

Abstract
Background Silverleaf sunflower, Helianthus argophyllus , is one of the most important wild species that have been usually used for the improvement of cultivated sunflower. Although a reference genome is now available for the cultivated species, H. annuus , its effect in helping understanding the mechanisms underlying the traits of H. argophyllus is limited by the substantial genomic variance between these two species.Results In this study, we generated a high-quality reference transcriptome of H. argophyllus using Iso-seq strategy. This assembly contains 50,153 unique genes covering more than 91% of the whole genes. Among them, we find 205 genes that are absent in the cultivated species and 475 fusion genes containing components of coding or non-coding sequences from the genome of H. annuus . It is interesting that in line with the strong disease resistance observed for H. argophyllus , these H. argophyllus -specific genes are predominantly related to functions of resistance. We have also profiled the gene expressions in leaf and root under normal or salt stressed conditions and, as a result, find distinct transcriptomic responses to salt stress in leaf and root. Particularly, genes involved in several critical processes including the synthesis and metabolism of glutamate and carbohydrate transport are reversely regulated in leaf and root.Conclusions Overall, this study provided insights into the genomic mechanisms underlying the disease resistance and salt tolerance of silverleaf sunflower and the transcriptome assembly and the genes identified in this study can serve as a complement data resources for future research and breeding programs of sunflowers.
Supervised exercise protocol for lower limbs in subjects with chronic venous disease: an evaluator-blinded randomized clinical trial
Authors: Volpe Esther Tinoco , Resqueti Vanessa Regiane , Silva Ana Aline Marcelino , Gualdi Lucien Peroni , Fregonezi Guilherme Augusto de Freitas 
Published: November 2019

Abstract
Background: Chronic venous insufficiency (CVI) causes pathophysiological changes in lower limb muscles, particularly calf muscles, and limits ankle range of motion (ROM). These changes reduce functional activities and decreases quality of life (QOL). Although several studies have shown benefits of exercise (strengthening the calf muscles to improve calf muscle pumping and QOL) in patients with CVI, few studies are randomized controlled trials. This has led to a weak indication of exercise for the treatment of patients with CVI. The aim of this study is to analyze the effects of a supervised exercise program to improve calf muscle endurance as well as QOL in patients with CVI. Methods: This is an evaluator-blind, randomized clinical trial with an 8-week duration and a follow-up evaluation at week 16. A pilot study with subjects with CVI diagnosis will be performed to calculate sample size. The participants will be randomly allocated (1:1) into a treatment or control group (usual care/no intervention). The treatment intervention consists of a bi-weekly supervised exercise program of the lower limbs that will include aerobic training, strengthening, and cardiovascular exercises. The participants from both groups will participate in a health education lecture. Primary outcomes are changes in calf muscle endurance and QOL score. Secondary outcomes are changes in exercise capacity, ankle ROM, electrical muscle activity and cardiac output. Discussion: Patients with CVI may have an impaired calf muscle pump and decreased exercise capacity. A randomized controlled trial evaluating a supervised exercise program will provide much needed information on the management of CVI to promote health and independence. Trial registration: This study was registered on the Brazilian Clinical Trials Database (REBEC) (RBR-57xtk7). The results will be disseminated at scientific events, presentations, and publications in peer reviewed journals. Keywords: Exercise, Resistance Training, Exercise Therapy, Venous Insufficiency, Randomized Controlled Trial, Rehabilitation
Experimental investigation of photovoltaic module system coupled with solar panel cleaning system
Authors: Katakam Sai Manoj , Atheaya Deepali , Aligireddy Susheel Reddy , Guptaa Yash , Bhukhari Adil Ashraf 
Published: November 2019

Solar energy is a non-conventional source of energy. Solar panels generate electrical output by absorbing solar radiation. The performance of photovoltaic (PV) module often depends on environmental factors like dust, humidity, hail and temperature. Installation elements like altitude, installation site, tilt angle also affects the efficiency of solar energy output. The objective of this research work is to clean the dust deposited on PV panels using the minimum amount of water and energy. To regularly clean the dust, a semi-automatic self-cleaning mechanism has been designed and fabricated, which cleans the deposited dust on the solar panel automatically. Therefore, the process turns out to be reliable and faster and it leads to increased power output of the solar panels. An experimental investigation has been done at Bennett University, Greater Noida dated 15 October to 21st October 2019. An increase in efficiency by 1.2 % to 3 % was reported in this experimental study.
Feasibility study on the application of carbon dioxide phase change fracturing technology in a foundation pit of an open cut tunnel
Authors: Liu Shijie , Huang Zhenghong 
Published: November 2019

Carbon dioxide phase change fracturing technology is a brand-new and efficient cracking and tunneling technology. Because the method utilizes the change of carbon dioxide state, it belongs to a pure physical process. By adjusting the release rate of gaseous substances, it can effectively control the pressure of the gas, and achieve micro vibration, zero flying stone, zero toxic gas emission, and no micro harmful effect of shock wave, but the application of carbon dioxide phase change cracking technology in urban foundation pit excavation is less. In this paper, through the analysis of three different cracking parameters of urban foundation pit excavation process, it is found that when the diameter of the cracked pipe is 85 mm, the construction safety can be guaranteed. In addition, according to the on-site cracking effect, the carbon dioxide phase change cracking technology is a safe, reliable, relatively economical and efficient excavation technology under urban complex environmental conditions, and will not affect the surrounding environment during use, but Its cracking efficiency has a great relationship with the air surface.
PCA based health indicator for remaining useful life prediction of wind turbine gearbox
Authors: Mithun Praveen Hemanth , Shah Divya , Pandey Krishna Dutt , I Vamsi , G R Sabareesh 
Published: November 2019

Fault prognosis of wind turbine gearbox has received considerable attention as it predicts the remaining useful life which further allows the scheduling of maintenance strategies. However, the studies related towards the RUL prediction of wind turbine gearbox are limited, because of the complexity of gearbox, acute changes in the operating conditions and non-linear nature of the acquired vibration signals. In this study, a health indicator is constructed in order to predict the remaining useful life of the wind turbine gearbox. Run to fail experiments are performed on a laboratory scaled wind turbine gearbox of overall gear ratio 1:100. Vibration signals are acquired and decomposed through continuous wavelet transform to obtain the wavelet coefficients. Various statistical features are computed from the wavelet coefficients which return form high-dimensional input feature set. Principal component analysis is performed to reduce the dimensionality and principal components (PCs) are computed from the input feature set. PC1 is considered as the health indicator and subjected to further smoothening by linear rectification technique. Exponential degradation model is fit to the considered health indicator and the model is able to predict the RUL of the gearbox with an error percentage of 2.73 %.
Evaluation of Bioactive Component, Free Radical Scavenging Potentials and Protein Qualities of Gomphrena celosoides and Zea mays Leaves
Authors: Adaeze Bob-Chile A. , Amadi Peter U. 
Published: November 2019

Background:
The assessment of underexploited leaves has become crucial to supplement the
rapidly depleting sources of bioactive components as well as provide available nutrient sources for local
inhabitants.

Methods:
This study thus investigated the bioactive components of the oil, and fatty acid composition,
free radical scavenging potentials, and protein qualities of leaves of Z. mays and G. celosioides using
standard methods. The bioactive components of the oils and fatty acids were determined by Gas Chromatograpy,
while the amino acid and in-vitro antioxidant potentials were determined using a Technicon
Sequential Multi-Sample (TSM) Amino Acid Analyzer, and spectrophotometer, respectively.

Results:
The Z. Mays leaves showed the abundance of farnesene, hexadecanoic acids, and caryophellene
while G. celosioides produced high level of octadecadienoic acid, hexadecanoic acid, and phytol. Z.
mays and G. celosioides contained 72.48% and 60.55% unsaturated fatty acids respectively, with the
abundance of linolenic acid for Z. mays and oleic acid for G. celosioides. The result for the in vitro antioxidant
% inhibition showed a concentration dependent free radical scavenging potentials of the leaves.
Both G. celosioides and Z. mays produced greater 1,1-diphenyl-2- picrylhydrazyl (DPPH), and hydrogen
peroxide radical scavenging potentials than ascorbic acid, while at 40ppm the nitric oxide and 2,2-
azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical % inhibition of Z. mays leaves were
lower than those for ascorbic acid.

Discussion:
The number of essential amino acids in both plants were 48.20 and 39.25 g/100g, total
branched chain amino acids (TBCAA) were 21.15 and 16.92 g/100g, predicted protein efficiency ratios
(P-PERs) were in the range of 3.02-3.23 and 2.68-2.77, and the essential amino acid index (EAAI) were
1.52 and 1.48, for Z. mays and G. celosioides leaves respectively.

Conclusion:
From these results, the utilization of Z. mays and G. celosioides for high quality protein,
unsaturated fatty acids and potent antioxidant sources, should be massively encouraged.
Tipificación SARM en trabajadores asistenciales de las unidades de cuidados intensivos en una institución hospitalaria.
Authors: Ferreira C María Eugenia , Valencia J Karina , Buelvas D Francisco Alberto , Tovar A Catalina , Raciny A Mayra Ligia 
Published: November 2019

Objective. To characterize microbiologically MRSA in health care workers of the Intensive Care Units at a hospital institution in the city of Montería. Materials and methods. A longitudinal descriptive study was carried out in 52 workers from two intensive care units; nasal and pharyngeal swabs were taken from each individual, with a periodicity of 15 days / five months. Examined through microbiological methods such as fermentation of mannitol, catalase and coagulase the identification of S. aureus was performed, with the cefoxitin disc by Kirby-Bauer disc-diffusion method the methicillin resistance mediated by the mecA gene was evaluated; antibiotics such as Vancomycin, Erythromycin and Clindamycin were evaluated by this test and confirmed by MicroScan ®. Results. we obtained 88 isolates of Staphylococcus aureus and 21 MRSA, a prevalence of MRSA of 25%. 90.5% of strains Of SARM were recovered from the pharynx, in a greater proportion (50%) in nursing staff. Conclusions. Evidence of MRSA circulation in care staff within a hospital institution generates an alert that allows it to establish containment policies to avoid the dissemination of these strains and to avoid the appearance of outbreaks or hospital infections associated with this pathogen.
Emociones de los adolescentes internados en un centro de orientación juvenil. Córdoba – Colombia.
Authors: Amador A Concepción E , Avendaño V Angélica , Caldera O Karina 
Published: November 2019

Objective. To identify the emotions that adolescents have about your stay in the Youth Guidance Center. Materials and methods. Using qualitative methods, consent was addressed was informed of the institution, professional caregivers and los16 hospitalized adolescents was applied by direct interview a semistructured instrument validated by experience and a field diary to record the relations of verbal responses with no verbal. Documented analyses using the 34 semantic expressions were grouped by similarity of content until 4 categories of interest to the study. Results. Teenagers have positive emotions regarding your stay; they consider this offer personal security, confidence and satisfaction of their daily basic needs. In the category of interpersonal relationships with peers own personal-social sphere adolescent the presence of negative or unpleasurable emotions among those with personal history of emotional deprivation identified; on the contrary among those with affective previous history they showed better relationships with peers who come to consider as part of their families. Conclusions. The internment of adolescents apparently alters the emotional dimension in the social field staff who have not had previous emotional ties.
Respuesta inmune humoral contra la proteína recombinante r200L de Plasmodium vivax.
Authors: Yasnot A María Fernanda , Sánchez L Yeliana Lucía 
Published: November 2019

Objectives. To determine the humoral immune response to recombinant protein r200L of Plasmodium vivax in malarial infected and exposed individuals to residing in Tierralta - Córdoba. Linking demographic variables (gender, age, previous malarial); with the humoral response to protein produced by Plasmodium vivax r200L. Materials and Methods. The sampling was done for convenience, taking 84 samples, 42 were for patients infected with P. vivax, and 42 samples from people exposed to infection, all collected in Tierralta-Cordoba, the procedure was done using the technique of enzyme immunoassay (ELISA), the antibodies titre was related to demographic characteristics (gender, age, previous malarial), of the study population. Results. In both populations, the patient and exposed it became clear that the immune response was higher in the titles of 1:800, in some patients presenting high titles of antibodies to 1:3200. Conclusions. There is natural antigenicity to protein r200L of P. vivax in the study population and found not direct relationship with sociodemographic variables studied population.
Editorial
Authors: SÁNCHEZ CARABALLO ÁLVARO A 
Published: November 2019

Hace trescientos años, los investigadores rehuían publicar las conclusiones de sus estudios, ocultándolos, con la única finalidad de que nadie se apropiara de ellas. No obstante, en nuestros tiempos, la mejor manera de resguardar el producto de una investigación y salir del anonimato es publicando los resultados. Así, nacieron las primeras revistas de publicación científica. Desde entonces, publicar es crucial para el incremento y desarrollo científico.En algunos países de América Latina existe un desarrollo importante de la actividad científica. Sin embargo, en estas naciones la producción de conocimiento es incipiente, comparado con países desarrollados. Probablemente esta diferencia en la fecundación del conocimiento puede estar relacionada con el tipo de inclusión social, organización y avances de cada país. Una manera de acercarse al desarrollo científico de los países, está relacionada con los aportes realizados desde la academia y la comunicación de los hallazgos obtenidos en los procesos investigativos. Por tanto, la puesta en marcha de la Revista Avances en Salud, posibilita un espacio en el marco de la divulgación del trabajo investigativo.Pensando en lo anterior, la Facultad Ciencias de la Salud de la Universidad de Córdoba planificó el tener una revista con la suficiente calidad como para que su proyección fuera, al menos, de carácter nacional. Tal pensamiento, nutrido por los principios nucleares del periodismo científico, como son la producción literaria original, el respeto por la autoría, la revisión por pares idóneos y la conservación de la ética de la investigación, es la piedra angular que soporta y sustenta el esfuerzo que hoy se materializa, al ver la luz el primer número de la Revista Avances en Salud de la Facultad Ciencias de la Salud de la Universidad de Córdoba.El desafío, pasando por el reto académico e intelectual y con el insustituible apoyo de la Universidad de Córdoba, es robustecer la calidad de la revista y potenciar su perceptibilidad nacional. Algo que, con dedicación y esfuerzo, señalará el norte de esta publicación que tendrá una frecuencia inicial semestral y que espera responder al cada vez más competente y selecto grupo de lectores interesados en temas de salud.Con la revista Avances en Salud, se reafirma que la necesidad de información y comunicación en el establecimiento de redes investigativas para la comunidad científica, es una oportunidad que se pretende fortalecer, con la puesta en marcha de la misma. Reto académico este, que se espera sea asumido por nuestros lectores y por la comunidad académica y científica en general, quien debe posibilitar un proceso de integración y orientación en el área de las ciencias de la salud.Con la pretención de responder a las expectativas generadas, queda a su disposición este primer número de la revista Avances en Salud de la Facultad Ciencias de la Salud de la Universidad de Córdoba.
Factibilidad educativa en una institución universitaria.
Authors: Campos Gómez Lenis , Hanna Lavalle Mónica 
Published: November 2019

Objective. To determine the professional resources that enables the educational offer to a university program. Make a diagnosis through the D.O.F.A matrix to propose strategies required in decision- making. Materials and methods. Quantitative and cross-sectional study design. The population consisted of all professionals in the area attending a training event health. The information was collected through a structured questionnaire designed by the researchers. Results. The highest proportion were professionals residing in towns of Cordoba, owned nursing degree, worked in public institutions for the provision of services and activities exercised administrative nature. They showed interest in studies of Corporate Audit Quality in Health in quality management and specialization in Occupational Health also eligible for financing. The hue Dofa highlighted as strengths that the institution had the requirements for the proper provision of continuing education and take into account the real needs of the customer. In addition studies of competitiveness strategies to meet competition and technological surveillance to make alternative institutions. Conclusions. It was concluded that there is still no saturation of the labor market this in terms of the offered specializations.
Determinantes culturales de la salud. Opinión Novel.
Authors: González Fernández Ana , Genes Martínez Ariel , Mendoza Blanco José , Mera Zapata Rossy , Gaitán Vásquez Nany , Salazar Luna Zuly Candelaria 
Published: November 2019

Los determinantes de salud son propiedades basadas en el estilo de vida afectadas por amplias fuerzas sociales, económicas y políticas que influyen la calidad de la salud personal (1). Dentro de este grupo de propiedades se encuentran en un plano distal los determinantes culturales, los cuales son indispensables para el abordaje y entendimiento del proceso salud-enfermedad en una sociedad. A pesar de no existir una definición concreta sobre determinantes culturales, en el ejercicio para aproximarnos en la construcción de este, es conveniente primeramente definir el concepto de cultura, la cual, es entendida como conjunto de valores, costumbres, creencia y prácticas que constituyen la forma de vida de un grupo específico (2). Luego entonces, la cultura como determinante, son todas las formas y expresiones que caracterizan una población y determinan su estado de salud, tales como, etnicidad, el nivel educativo, género, religión, lengua, cosmovisión, arquetipos, creencias, costumbres y valores.
El derecho a la salud en Colombia: evolución y defensa frente a un estado que dista de ser garante.
Authors: Julio Pretelt José Domingo 
Published: November 2019

En Colombia, el proceso y manejo que se le ha dado al derecho a la salud no ha sido uniforme. Antes de la sentencia T-760 de 2008, ya el derecho a la salud existía consagrado directa e indirectamente en la Constitución Política de 1991, en varios de sus artículos, entre ellos el artículo 44, 48, 49, 365 y 366, entre otros relacionados. Pese a ello, las vulneraciones sistemáticas realizadas al derecho a la salud de los colombianos hicieron que en el país hubiera un viraje en la forma de cómo garantizar la salud y es así como este derecho fue adecuando a las intenciones del constituyente y posteriormente tomando una posicion categórica por los honorables jueces de la República, a tal punto que la salud en Colombia, pasó de ser un derecho social, económico y cultural a ser un derecho fundamental por conexidad y finalmente un derecho Fundamental autónomo.En este artículo, veremos un breve resumen de su evolución y los mecanismos actuales para defenderla, así como posibles formas de defender la salud en Colombia teniendo en cuenta la normativa nacional e internacional vigente.