bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient removal of three dyes using porous covalent triazine frameworks: adsorption mechanism and role of pore distribution
Authors: An Fengxia , Liu Jingliang , Xu Zhaoyi , Zheng Shourong 
Published: November 2020

Abstract
Dyes are widely used in production and life. In this study, porous covalent triazine frameworks (CTFs) were synthesized and the adsorption behavior for three dyes was investigated by batch adsorption experiments. CTFs were characterized by various spectroscopic techniques for structure, porosity and surface properties. Several possible adsorption mechanisms were proposed including pore-filling, electrostatic attraction and hydrogen bonding interaction with the triazine structure of CTFs. The mechanisms were further verified by the pore size distribution and pH dependence. Additionally, CTFDCBP displayed stronger adsorption affinity and faster adsorption kinetics for dyes, because of the wide pore size distribution. This study provides a new insight into the mesoporous CTFs, which exhibit great potential as an effective adsorbent for dye removal.
Phosphocreatine Promotes Osteoblastic Activities in H2O2-Induced MC3T3-E1 Cells by Regulating SIRT1/FOXO1/PGC-1α Signaling Pathway
Authors: Jing Zheng , Wang Changyuan , Wen Shijie , Jin Yue , Meng Qiang , Liu Qi , Wu Jingjing , Sun Huijun , Liu Mozhen 
Published: November 2020

Background:
Osteoporosis, characterized by bone loss, usually occurs with the increased
bone resorption and decreased bone formation. H2O2-induced MC3T3-E1 cells are commonly used
for the study of osteoblastic activities, which play a crucial role in bone formation.

Objective:
This study aimed to investigate the effects of Phosphocreatine (PCr) on the osteoblastic
activities in H2O2-induced MC3T3-E1 cells and elaborate on the possible molecular mechanism.

Methods:
The Osteoprotegerin (OPG)/Receptor Activator of NF-κB Ligand (RANKL) ratio and osteogenic
markers were detected to investigate the effects of PCr on osteoblastic activities, and the
osteoblastic apoptosis was detected using Hochest staining. Moreover, oxidative stress, Adenosine
Triphosphate (ATP) generation and the expression of Sirtuin 1 (SIRT1), Forkhead Box O 1
(FOXO1) and Peroxisome Proliferator-Activated Receptor Γ Coactivator-1α (PGC-1α) were also
examined to uncover the possible molecular mechanism in H2O2-induced MC3T3-E1 cells.

Result:
The results showed that PCr promoted the osteoblastic differentiation by increasing the expression
levels of osteogenic markers of Alkaline Phosphatase (ALP) and Runt-related transcription
factor 2 (Runx2), as well as increased the OPG/RANKL ratio and suppressed the osteoblastic apoptosis
in H2O2-induced MC3T3-E1 cells. Moreover, treatment with PCr suppressed reactive oxygen
species (ROS) over-generation and promoted the ATP production as well as increased the PGC-1α,
FOXO1 and SIRT1 protein expression levels in H2O2-induced MC3T3-E1 cells.

Conclusion:
PCr treatment could promote osteoblastic activities via suppressing oxidative stress
and increasing the ATP generation in H2O2-induced MC3T3-E1 cells. In addition, the positive effects
of PCr on osteoblasts might be regulated by SIRT1/FOXO1/ PGC-1α signaling pathway.
Neuropeptidomics: Improvements in Mass Spectrometry Imaging Analysis and Recent Advancements
Authors: Vu Nhu Q. , DeLaney Kellen , Li Lingjun 
Published: November 2020

:
Neuropeptides are an important class of endogenous peptides in the nervous system that
regulate physiological functions such as feeding, glucose homeostasis, pain, memory, reproduction,
and many others. In order to understand the functional role of neuropeptides in diseases or disorders,
studies investigating their dysregulation in terms of changes in abundance and localization
must be carried out. As multiple neuropeptides are believed to play a functional role in each physiological
process, techniques capable of global profiling multiple neuropeptides simultaneously are
desired. Mass spectrometry is well-suited for this goal due to its ability to perform untargeted measurements
without prior comprehensive knowledge of the analytes of interest. Mass spectrometry
imaging (MSI) is particularly useful because it has the capability to image a large variety of peptides
in a single experiment without labeling. Like all analytical techniques, careful sample preparation
is critical to successful MSI analysis. The first half of this review focuses on recent developments
in MSI sample preparation and instrumentation for analyzing neuropeptides and other biomolecules
in which the sample preparation technique may be directly applicable for neuropeptide
analysis. The benefit offered by incorporating these techniques is shown as improvement in a number
of observable neuropeptides, enhanced signal to noise, increased spatial resolution, or a combination
of these aspects. The second half of this review focuses on recent biological discoveries
about neuropeptides resulting from these improvements in MSI analysis. The recent progress in
neuropeptide detection and analysis methods, including the incorporation of various tissue washes,
matrices, instruments, ionization sources, and computation approaches combined with the advancements
in understanding neuropeptide function in a variety of model organisms, indicates the potential
for the utilization of MSI analysis of neuropeptides in clinical settings.
Development and Biocompatibility Analysis of NBD Peptide Sustained- Release Microsphere Scaffold Nanoparticle SP-Sr-CaS/NBD
Authors: Li Xue , Wang Lei , Wang Changbing , Tan Caixia , Liu Xifaofang , Zhu Yongzhan 
Published: November 2020

Background:
In clinical treatment, it is difficult to carry out effective bone tissue transplantation
and anti-inflammatory treatment at the same time due to bone defects and osteomyelitis
where the tissue is contaminated or infected. As a downstream target of TNF-α, NF-κB has an inhibition
effect on the proliferation and differentiation of cells surrounding the lesion. As a negative effect,
it leads to a reduction in bone growth and development.

Methods:
In this study, the small molecule NBD polypeptide and bone conduction matrix Sr-CaS
are microspheres, formed to prepare Sr-CaS, NBD drug-loaded sustained-release microspheres in
order to achieve a Sr-CaS/NBD peptide drug-loaded sustained release microsphere scaffold material
(SP-Sr-CaS/NBD). We prepared the microspheres and optimized the production process to obtain
particles with stable morphological properties and sustained release properties.

Results:
In vitro experiments demonstrated that SP-Sr-CaS/NBD could reduce TNF-α-induced cell
growth inhibition, caspase-3 activity and NF-κB transcriptional activation as the function of continuous
NBD peptide dosing regimen.

Conclusion:
Also, the introduction of the Sr-CaS matrix potentiates microspheres to promote cell
proliferation and provides a basis to become a promising 3D bone scaffold material in the future.
Bioinformatics-Based Identification of Selective Cationic Amphipatic Antibacterial Peptides: Aurein 1.2 Variants Case
Authors: Polanco Carlos , Huberman Alberto , Uversky Vladimir N. , Andrés Leire , Buhse Thomas , Castañón-González Jorge Alberto , Díaz-González Juan Luciano 
Published: November 2020

Background:
Selective Cationic Amphipathic Antibacterial Peptides (SCAAPs) occupy a prominent place in the
production of new drugs on account of their high toxicity towards bacteria and low toxicity towards mammalian cells, low
hemolytic activity, and contribution to the protection of the human immune system.

Aim:
their number in nature is very low, and experimental tests are very protracted and costly.
Therefore, it would be useful to have bioinformatics tools that would identify them in the existing
databases and also propose new synthetic SCAAPs.

Methods:
In order to reduce the costs of identification and/or chemical synthesis and to know the
physicochemical characteristics of SCAAPs at a residues level and to obtain a “bioiformatics fingerprint”
suitable for their selection, we have modified the Polarity Index Method® (PIM®) and the
α-helical configuration of each sequence is included in determining their individual “PIM®
profile”. We have also used a set of the computer program to determine their “Intrinsic Disorder
Predisposition”. This information was then compared with other protein groups, such as bacteria,
fungi, virus and Cell-Penetrating Peptides (CPP) from the UniProt database and a set of intrinsically
disordered proteins. Once the “fingerprint” of SCAAPs was obtained, it was used for searching
among the 559228 “reviewed” proteins from the UniProt database and a set of synthetic SCAAPs
characterized by the predefined “PIM® profile” selected.

Results:
Our results showed that the metric named “PIM® profile” can identify, with a high level of accuracy, a group of
bacterial SCAAPs. This bioinformatics study was supported at residues level, using the in-house bioinformatics system
Polarity Index Method the commonly used algorithm for the prediction of intrinsic disorder predisposition, PONDR®
FIT.

Conclusions:
The Polarity Index Method seems highly efficient identifying SCAAP candidates.
A Patient with COVID-19 Pneumonia Presenting with Plural Effusion: A Case Report
Authors: Ghiasvand Fereshteh , SeyedAlinaghi SeyedAhmad , Tirgar Samaneh , Salehi Mohammad Reza , Moradmand-Badie Banafsheh 
Published: November 2020

Background:
In February 2020, coronavirus disease (COVID-19) emerged in Tehran,
Iran.

Case Presentation:
Herein, we reported clinical features, laboratory tests, unusual radiological
characteristics and therapeutic course of a patient with initial mild clinical symptoms at presentation
with progression to pneumonia and pleural effusion in the emergency unit of a referral hospital.
Substance Use Portrayal in Oscar-nominated Movies
Authors: Castaldelli-Maia João Mauricio , Gil Felipe , Ventriglio Antonio , Torales Julio , Florio Ligia , Moura Helena F. , de Andrade Arthur Guerra , Lotufo-Neto Francisco , Bhugra Dinesh 
Published: November 2020

Background:
As one of the forms of media and art most consumed in the world, Oscar-
nominated movies should have their drug use representation monitored because of possibly influencing
but also reflecting society’s behavior.

Objective:
The present study aims to investigate drug use representation in scenes from movies
nominated for the Academy Awards (Oscar) from 2008-2011 through media content analysis.

Methods:
437 scenes from Oscar-nominated movies (best film, best actor and best actress categories)
showing drug consumption and/or its effects were assessed. Each drug represented and identified
in a given scene (i.e., drug use incident) was counted as a unit for the present study (n = 515).
Survey settings were used to control for over- or under-estimation of the prevalence of a variable in
a given year or movie.

Results:
All the Oscar-nominated movies portrayed at least one scene of drug use. There was a
massive predominance of alcohol and tobacco in movies, with a high use among men who also use
drugs, habitually or occasionally, but related to stress/tension, predominantly at home. However,
there was a significant progressive increase in the use of drugs other than alcohol and tobacco, multiple
drugs, and by women.

Conclusion:
These findings echo epidemiological studies on substance use in western countries, an overall trend towards greater home drug use representation and gender convergence since 1970, which increased since 2000. Monitoring drug use representation in Oscar-nominated movies may represent an important public health tool.
Plano de pagamento
Authors: De Magalhães Celso 
Published: November 2020

A retribuição pecuniária percebida pelo empregado particular e peloservidor público, como retribuição dos serviços prestados, é fator de sumaimportância em Administração de Pessoal.Essa retribuição deve satisfazer a duas condições fundamentais: terá de ser justa e terá de ser suficiente.Nãc importa que se revista da primeira característica, isto é, pouco se dáque essa remuneração seja justa; se não fôr também suficiente, fugirá à suafinalidade.Com efeito a retribuição pafa ao homem que trabalha deve garantir-lhenormalmente a estabilidade econômica, defendendo-o contra os embates constantesda vida
Accuracy of Monocular Two-Dimensional Pose Estimation Compared With a Reference Standard for Kinematic Multiview Analysis: Validation Study
Authors: Stamm Oskar , Heimann-Steinert Anika 
Published: November 2020

Background
Expensive optoelectronic systems, considered the gold standard, require a laboratory environment and the attachment of markers, and they are therefore rarely used in everyday clinical practice. Two-dimensional (2D) human pose estimations for clinical purposes allow kinematic analyses to be carried out via a camera-based smartphone app. Since clinical specialists highly depend on the validity of information, there is a need to evaluate the accuracy of 2D pose estimation apps.

Objective
The aim of the study was to investigate the accuracy of the 2D pose estimation of a mobility analysis app (Lindera-v2), using the PanopticStudio Toolbox data set as a reference standard. The study aimed to assess the differences in joint angles obtained by 2D video information generated with the Lindera-v2 algorithm and the reference standard. The results can provide an important assessment of the adequacy of the app for clinical use.

Methods
To evaluate the accuracy of the Lindera-v2 algorithm, 10 video sequences were analyzed. Accuracy was evaluated by assessing a total of 30,000 data pairs for each joint (10 joints in total), comparing the angle data obtained from the Lindera-v2 algorithm with those of the reference standard. The mean differences of the angles were calculated for each joint, and a comparison was made between the estimated values and the reference standard values. Furthermore, the mean absolute error (MAE), root mean square error, and symmetric mean absolute percentage error of the 2D angles were calculated. Agreement between the 2 measurement methods was calculated using the intraclass correlation coefficient (ICC[A,2]). A cross-correlation was calculated for the time series to verify whether there was a temporal shift in the data.

Results
The mean difference of the Lindera-v2 data in the right hip was the closest to the reference standard, with a mean value difference of –0.05° (SD 6.06°). The greatest difference in comparison with the baseline was found in the neck, with a measurement of –3.07° (SD 6.43°). The MAE of the angle measurement closest to the baseline was observed in the pelvis (1.40°, SD 1.48°). In contrast, the largest MAE was observed in the right shoulder (6.48°, SD 8.43°). The medians of all acquired joints ranged in difference from 0.19° to 3.17° compared with the reference standard. The ICC values ranged from 0.951 (95% CI 0.914-0.969) in the neck to 0.997 (95% CI 0.997-0.997) in the left elbow joint. The cross-correlation showed that the Lindera-v2 algorithm had no temporal lag.

Conclusions
The results of the study indicate that a 2D pose estimation by means of a smartphone app can have excellent agreement compared with a validated reference standard. An assessment of kinematic variables can be performed with the analyzed algorithm, showing only minimal deviations compared with data from a massive multiview system.
Effects of a Personalized Fitness Recommender System Using Gamification and Continuous Player Modeling: System Design and Long-Term Validation Study
Authors: Zhao Zhao , Arya Ali , Orji Rita , Chan Gerry 
Published: November 2020

Background
Gamification and persuasive games are effective tools to motivate behavior change, particularly to promote daily physical activities. On the one hand, studies have suggested that a one-size-fits-all approach does not work well for persuasive game design. On the other hand, player modeling and recommender systems are increasingly used for personalizing content. However, there are few existing studies on how to build comprehensive player models for personalizing gamified systems, recommending daily physical activities, or the long-term effectiveness of such gamified exercise-promoting systems.

Objective
This paper aims to introduce a gamified, 24/7 fitness assistant system that provides personalized recommendations and generates gamified content targeted at individual users to bridge the aforementioned gaps. This research aims to investigate how to design gamified physical activity interventions to achieve long-term engagement.

Methods
We proposed a comprehensive model for gamified fitness recommender systems that uses detailed and dynamic player modeling and wearable-based tracking to provide personalized game features and activity recommendations. Data were collected from 40 participants (23 men and 17 women) who participated in a long-term investigation on the effectiveness of our recommender system that gradually establishes and updates an individual player model (for each unique user) over a period of 60 days.

Results
Our results showed the feasibility and effectiveness of the proposed system, particularly for generating personalized exercise recommendations using player modeling. There was a statistically significant difference among the 3 groups (full, personalized, and gamified) for overall motivation (F3,36=22.49; P<.001), satisfaction (F3,36=22.12; P<.001), and preference (F3,36=15.0; P<.001), suggesting that both gamification and personalization have positive effects on the levels of motivation, satisfaction, and preference. Furthermore, qualitative results revealed that a customized storyline was the most requested feature, followed by a multiplayer mode, more quality recommendations, a feature for setting and tracking fitness goals, and more location-based features.

Conclusions
On the basis of these results and drawing from the gamer modeling literature, we conclude that personalizing recommendations using player modeling and gamification can improve participants’ engagement and motivation toward fitness activities over time.
Virtual Overdose Response for People Who Use Opioids Alone: Protocol for a Feasibility and Clinical Trial Study
Authors: Bristowe Sean Kristina , Ghosh Sumantra Monty , Trew Michael ,   Canadian Mental Health Association - Calgary Region, Rittenbach Katherine 
Published: November 2020

Background
A recent quarterly report released by Alberta Health reports that on average 2.5 Albertans die every day from accidental overdose deaths, and that between April 1, 2020, and June 30, 2020, the province lost a total of 301 people. In Canada, between January 2016 and March 2020, a total of 16,364 people died due to opioid-related overdose. The World Health Organization reports that 70% of the 0.5 million deaths worldwide caused by drugs are related to opioid overdose. Although supervised consumption sites or safe injection sites have been shown to be effective in reducing the harms associated with the use of illicit substances and increasing uptake of addiction treatment and other health services, there is still significant stigma associated with them, and it is unlikely that all of the people who would benefit from supervised consumption service will ever access a site.

Objective
To help prevent deaths in populations that cannot or will not access physical safer consumption services in Alberta, we propose to provide virtual (telephone-based) overdose response services, staffed by people with lived experience.The primary outcome for this study is uptake of the service as measured by the number of calls to the service. Secondary outcomes will include patterns of use of the phone line (days of the week and time of calls) and outcomes from the calls (number of emergency medical services dispatches for overdoses from the service and the results of those dispatches).

Methods
This phase 1 clinical study is set to officially launch in early May 2020. The service will be available to up to 15 participants who self-disclose as using opioids unobserved and have given informed consent for both data collection and interviews. This group will have access to a toll-free telephone number and be invited to call when they plan to use opioids alone.

Results
The analysis will include mixed methods. To improve the design of the service and ensure safety of all involved, quantitative data will be collected on phone calls and participant health care usage, while qualitative data will be collected from both participants and virtual overdose response operators

Conclusions
This clinical trial aims to test the feasibility of a service that provides virtual overdose response in order to help prevent deaths in populations that cannot or will not access physical supervised consumption services in Alberta.

Trial Registration
ClinicalTrials.gov NCT04391192; https://www.clinicaltrials.gov/ct2/show/NCT04391192

International Registered Report Identifier (IRRID)
DERR1-10.2196/20183
Evaluating the Onset, Severity, and Recovery of Changes to Smell and Taste Associated With COVID-19 Infection in a Singaporean Population (the COVOSMIA-19 Trial): Protocol for a Prospective Case-Control Study
Authors: Sheen Florence , Tan Vicki , Haldar Sumanto , Sengupta Sharmila , Allen David , Somani Jyoti , Chen Hui Yee , Tambyah Paul , Forde Ciaran G 
Published: November 2020

Background
Sudden loss of smell and/or taste has been suggested to be an early marker of COVID-19 infection, with most findings based on self-reporting of sensory changes at a single time point.

Objective
To understand the onset, severity, and recovery of sensory changes associated with COVID-19 infection, this study will longitudinally track changes in chemosensory acuity among people with suspected COVID-19 infection using standardized test stimuli that are self-administered over 28 days.

Methods
In a prospective, case-controlled observational study, volunteers will be recruited when they present for COVID-19 screening by respiratory tract polymerase chain reaction test (“swab test”). The volunteers will initially complete a series of questionnaires to record their recent changes in smell and taste ability, followed by a brief standardized smell and taste test. Participants will receive a home-use smell and taste test kit to prospectively complete daily self-assessments of their smell and taste acuity at their place of residence for up to 4 weeks, with all data submitted for collection through web-based software.

Results
This study has been approved by the Domain Specific Review Board of the National Healthcare Group, Singapore, and is funded by the Biomedical Research Council Singapore COVID-19 Research Fund. Recruitment began on July 23, 2020, and will continue through to March 31, 2021. As of October 2, 2020, 69 participants had been recruited.

Conclusions
To our knowledge, this study will be the first to collect longitudinal data on changes to smell and taste sensitivity related to clinically diagnosed COVID-19 infection, confirmed by PCR swab test, in a population-based cohort. The findings will provide temporal insights on the onset, severity, and recovery of sensory changes with COVID-19 infection, the consistency of symptoms, and the frequency of full smell recovery among patients with COVID-19. This self-administered and cost-effective approach has many advantages over self-report questionnaire–based methods and provides a more objective measure of smell and taste changes associated with COVID-19 infection; this will encourage otherwise asymptomatic individuals who are potential spreaders of the virus to self-isolate and seek formal medical diagnosis if they experience a sudden change in sensory acuity. This broadened case finding can potentially help control the COVID-19 pandemic and reduce the emergence of clusters of infections.

Trial Registration
ClinicalTrials.gov NCT04492904; https://clinicaltrials.gov/ct2/show/NCT04492904.

International Registered Report Identifier (IRRID)
DERR1-10.2196/24797