bluuebunny/crossref_metadata_2025_split huggingface dataset:
SV40T reprograms Schwann cells into stem-like cells that can re-differentiate into terminal nerve cells.
Authors: Li Rui-Fang , Nan Guo-Xing , Wang Dan , Gao Chang , Yang Juan , He Tong-Chuan , Zhang Zhong-lin 
Published: February 2020

Abstract
Background : The effects of Simian virus 40 T antigen (SV40T) on various kinds of cells are different. Previous researchers failed to use SV40T immortalized nerve cells. However, they argued that SV40T caused nerve cell transformation. No one further study what is the specific effect of SV40T on nerve cells. We transfected Schwann cells (SCs) that did not have differentiation ability with MPH 86 plasmid containing SV40T in order to explore the effects of SV40T on Schwann cells. Methods: SCs were transfected with MPH 86 plasmid carrying the SV40T gene and cultured in different media, as well as co-cultured with neural stem cells (NSCs). In our study, SCs overexpressing SV40T were defined as SV40T-SCs. The proliferation of these cells was detected by WST-1, and the expression of different biomarkers was analyzed by qPCR and immunohistochemistry. Results: SV40T induced the characteristics of NSCs, such as the ability to grow in suspension, form spheroid colonies and proliferate rapidly, in the SCs, which were reversed by knocking out SV40T by the Flip-adenovirus . In addition, SV40T up-regulated the neurocrest markers Nestin, Pax3 and Slug, and down-regulated S100b as well as the late differentiation markers MBP, GFAP and Olig1/2. These cells also expressed NSC markers like Nestin, SOX2, CD133 and SSEA-1, as well as early development markers of embryonic stem cells (ESCs) like BMP4, C-myc, OCT4 and Gbx2. Co-culturing with NSCs induced differentiation of the SV40T-SCs into neuronal and glial cells. Conclusions: SV40T reprograms Schwann cells to stem-like cells at the stage of neural crest cells that can differentiate to terminal nerve cells. Background : The effects of Simian virus 40 T antigen (SV40T) on various kinds of cells are different. Previous researchers failed to use SV40T immortalized nerve cells. However, they argued that SV40T caused nerve cell transformation. No one further study what is the specific effect of SV40T on nerve cells.
Wound healing potential: Evaluation of molecular profiling and amplification of Lucilia sericata angiopoietin-1 mRNA mid-part
Authors: Alipour Hamzeh , Shahriari-Namadi Marziae , Ebrahimi Saeedeh , Moemenbellah-Fard Mohammad Djaefar 
Published: February 2020

Abstract
Objective: High prevalence of chronic ulcers and burden of disease necessitate the increasingly significant production of new recombinant proteins in the world. The angiopoietin-1 enzyme is a part of growth factors group which is secreted by Lucilia sericata (Diptera: Calliphoridae) larvae when they are exposed to wounds to ensure maggot therapy. It is one of the most potent proteins in wound healing. Given its essential role, angiopoietin-1 gene of L. sericata was characterized which provided some basic information on its identity. Results: The mid-part of angiopoietin-1 mRNA sequence was thus characterized based on the design of disparate primers such as exon-exon junction, conserved regions, and specific region primers via conventional polymerase chain reaction (PCR). Its structural features was configured by in silico method. The sequence of mid-part (390 bp) of angiopoietin-1 was determined empirically, and BLAST analysis unravelled its high identity (85%) with the sequence of angiopoietin-1 mRNA of the larval housefly, Musca domestica . The homology of this enzyme also exhibited that its nucleic acid sequence was very similar to the domains of angiopoietin-1 in Lucilia cuprina . The current data are critical to evaluate the action of this enzyme in recombinant protein production in future molecular studies on wound healing.
Risk factors for bladder calculus in patients with benign prostatic hyperplasia: a retrospective single-center study
Authors: Sun Xin , Yu Dexin , Cao Zhangjun , Xie Dongdong , Bi Liangkuan 
Published: February 2020

Abstract
Background: To explore the risk factors that lead to the formation of bladder calculus in patients with benign prostatic hyperplasia (BPH). Methods: Retrospective study was performed between June 2017 and October 2019, 103 patients with BPH who underwent transurethral resection of the prostate (TURP) were included. Patients with BPH were divided into two groups: 32 patients with bladder calculus in group1 and 71 patients without bladder calculus in group2. Characteristics of both groups were compared and univariate and multivariate analyses were performed to investigate the association between BPH with or without bladder calculus. Results: There was no significant difference between the two groups regarding age, duration of BPH, body mass index (BMI), total prostate volume (TPV), total prostate-specific antigen (TPSA), International prostate symptom score (IPSS), serum albumin, hemoglobin, uric acid, urinary tract infection, diabetes, and hypertension. The patients' serum creatinine and acute urinary retention (AUR) were significantly lower, while intravesical prostatic protrusion (IPP) was significantly higher in group 1 than group 2. Multivariate analyses revealed that IPP and AUR were significant risk factors for the formation of bladder calculus in patients with BPH. For IPP, the receiver operating characteristic (ROC) curve showed that the cut-off value of the highest risk of the formation of bladder calculus was 11.5 mm. Conclusions: Our study indicated that IPP and AUR were independent risk factors that were closely linked with the incidence of bladder calculus in BPH patients.
Species diversity and spatial distribution of CL/VL vectors: Evidence for variation in gene expression of immunogenic SP15 and LeIF proteins due to bioclimatic characteristics and physiological status of sand flies, Iran
Authors: Bordbar Ali , Parvizi Parviz 
Published: February 2020

Abstract
Background Main approaches of this investigation were climate regionalization to recognize the spatial distribution of cutaneous/visceral leishmaniasis (CL/VL) vectors as risk-maps using ArcGIS modeling system, evaluation of species biodiversity, assessing bio-climate (BC) effect on expression plasticity of genes possessing vaccine properties isolated from wild-collected sand flies. Methods The Inverse Distance Weighting (IDW) interpolation method was used to obtain accurate geography map using ArcGIS10.3.1 in closely-related distances. Species diversity was calculated based on Shannon diversity index using Rv.3.5.3. Expression fold change of SP15 and LeIF genes was evaluated using cDNA synthesis and RT-qPCR analysis. Results Three BC zone were identified in northeast of Iran. Phlebotomus papatasi were abundantly captured in all BC regions and the frequency was more in plains areas of mountainous BC as hot spots of CL. VL vectors were more prevalent in spatial cluster of Mediterranean BC. Semi-arid BC was identified as a major contributing factor to up-regulate SP15 salivary gene expression ( P =0.0050, P <0.05), and Mediterranean BC had considerable effect on up-regulation of LeIF- Leishmania gene in gravid and semi-gravid P. papatasi population ( P =0.0109, P <0.05). Conclusions The diversity and spatial distribution of CL/VL vectors associated with BC regionalization obtained in our research provide epidemiological risk maps and establish more effectively control measures against leishmaniasis. Oscillations in gene expression indicate that each gene has its own features, which are profoundly affected by bioclimatic characteristics and physiological status of sand flies. It is essential to consider BC factors affecting regulatory regions of environmentally responsive loci for genes used in vaccine designing.
Dynamics of Health Technology Diffusion in Integrated Care System (DHTDICS): A Development and Validation Study
Authors: Deng Qingwen , Liu Wenbin 
Published: February 2020

Abstract
Background Limited diffusion and utilization of health technology has greatly halted the improvement of resource integration and healthcare outcomes. However, the dynamic mechanism of health technology diffusion in the context of integrated care system (ICS) remained largely unknown. The purpose of this study was to develop and validate the scale on Dynamics of Health Technology Diffusion in Integrated Care System (DHTDICS) for providing instruments to investigate the health technology diffusion in ICS.Methods The scale was initially designed on the basis of the proposed model developed from previous research. And it was validated in a cross-sectional questionnaire survey. Exploratory factor analysis was used to assess domains in the questionnaire, and analyzed factorials, internal consistency and validity of the questionnaire. Results Reliability analysis revealed excellent internal consistency, as the value of Cronbach’s alpha all greater than 0.80 for four of the domains in this study. An acceptable validity was confirmed through tests of construct validity, convergent validity and discriminant validity. With respect to the potential domains and dimensions that DHTDICS contributes, the results highlight the existence of 4 domains: personal beliefs, technical drivers, organizational readiness and external environment.Conclusions The findings of this study will be capable to serve as a valid instrument to measure health technology diffusion, and be also helpful in developing future intervention strategies to promote the health technology diffusion in ICS.
Genome relationships and LTR-retrotransposon diversity in three cultivated Capsicum L. (Solanaceae) species
Authors: de Assis Rafael , Baba Viviane Yumi , Cintra Leonardo Adabo , Gonçalves Leandro Simões Azeredo , Rodrigues Rosana , Vanzela Andre Luís Laforga 
Published: February 2020

Abstract
Background: Plant genomes are rich in repetitive sequences, and transposable elements (TEs) are the most accumulated of them. This mobile fraction can be distinguished as Class I (retrotransposons) and Class II (transposons). Retrotransposons that are transposed using an intermediate RNA and that accumulate in a “copy-and-paste” manner have been screened in three completely sequenced genomes of peppers (Solanaceae family). The goal of this study was to understand the genome relationships among Capsicum annuum , C. chinense and C. baccatum , based on a comparative analysis of the function, diversity and the chromosome distribution of TE lineages in the Capsicum karyotypes. Due to the great commercial importance of pepper in natura , as a spice or as an ornamental plant, these genomes have been widely sequenced, and all of the assemblies are available in the SolGenomics group. These sequences have been used to compare all repetitive fractions from a cytogenomic point of view. Results: The qualification and quantification of LTR-retrotransposons (LTR-RT) families are contrasted with molecular cytogenetics data, and the results show a strong genome similarity between C. annuum and C. chinense as compared to C. baccatum . The Gypsy superfamily is more abundant than Copia , especially for Tekay/Del family members, including a high representation in C. annuum and C. chinense . On the other hand, C. baccatum accumulates more Athila/Tat sequences. The FISH results show retrotransposons differentially scattered along chromosomes, with the exception of CRM family sequences, which mainly have a proximal accumulation associated with heterochromatin bands. Conclusions: The results confirm a close genomic relationship between C. annuum and C. chinense in comparison to C. baccatum . Centromeric GC-rich bands appeared to be associated with the accumulation regions of CRM elements, whereas terminal and subterminal AT- and GC-rich bands do not correspond to the accumulation of the retrotransposons in the three Capsicum species tested here.
Complete genome of the barotolerant Listeria monocytogenes RO15 strain and comparison with other strains isolated from food and food processing environments
Authors: Duru Ilhan Cem , Andreevskaya Margarita , Laine Pia , Rode Tone Mari , Ylinen Anne , Løvdal Trond , Bar Nadav , Crauwels Peter , Riedel Christian , Bucur Florentina Ionela , Nicolau Anca Ioana , Auvinen Petri 
Published: February 2020

Abstract
Background

Consumption of Listeria monocytogenes contaminated food can cause infection with a high mortality rate in humans and animals. High pressure processing (HPP) is a non-thermal preservation technique adopted by the food industry to inactivate food pathogens, including L. monocytogenes. Strains of L. monocytogenes show different level of resistance to the high pressure. Some strains resist up to 500 MPa pressure. Here, we tested the pressure resistance of 10 different L. monocytogenes strains, including field isolates and widely used type strains, to 400 and 600 MPa pressure treatments. Genome sequencing, and genome comparison of the tested L. monocytogenes strains were performed to investigate the relation between genomic profile and pressure resistance.

Results

In this study, we showed that none of the tested strains were resistant to 600 MPa, more than 5 log 10 reduction observed for all strains after 1 minute 600 MPa pressure treatment. However, L. monocytogenes strain RO15 showed no significant reduction in viable cell counts after 400 MPa for 1 minute and it was defined as barotolerant. Genome sequencing of so far unsequenced L. monocytogenes strain RO15, 2HF33, MB5, AB199, AB120, C7, and RO4 allowed us to compare their gene content. Genome comparison of 10 tested strains showed that the three most pressure tolerant strains had more than one CRISPR system with self-targeting spacers. Further, several anti-CRISPR genes were detected in these strains. Pan-genome wide analysis showed that 10 prophage genes were significantly associated with the three most barotolerant strains.

Conclusions

L. monocytogenes strain RO15 was the most pressure tolerant among the selected strains. Genome comparison suggests that there might be a relationship with prophages, CRISPR systems and pressure resistance in L. monocytogenes .
The Predictive value of serum NGAL for the diagnosis of Delayed Graft Function in kidney transplantation
Authors: Pourmand Gholamreza , Emamjomeh Behrouz , Gooran Shahram , Namdari Farshad , Mehrsay Abdolrasool , Dehghani Sanaz , Mashhadi Rahil , Aghaii Maryam , Bagherpour Farzaneh , Heidari Fariba , Ashtari Sara , Khatami Fatemeh , Aghamir Seyed Mohammad Kazem 
Published: February 2020

Abstract
Background and aims the role of serum neutrophil gelatinase-associated lipocalin (NGAL) in predicting delayed graft function (DGF) after kidney transplantation is poorly defined. The objective of this study was to evaluate the serum NGAL expression in the early postoperative phase after kidney transplantation and compare it with serum creatinine (Cr). Methods We studied 32 patients who received kidney transplantation from deceased (n=24) and live (n=5) donors during October 2017 to December 2018 at the Urology Research Center, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran. Serum NGAL, Serum Cr and urine output were measured at 1 to 7 day after transplantation. The need for dialysis in one week after transplantation was evaluated. Results Among 29 recipients with serum biomarkers measurements, 8 (27.5%) developed DGF (need to hemodialysis within 1 week of transplantation). Resulted in areas under ROC curves (AUCs) for serum NGAL at early hours following transplantation was (0.839, 95% CI: 0.69-0.98, P =0.005) that could accurately predict DGF compared to urine output (0.747, 95% CI: 0.55-0.93, P =0.045) and serum Cr (0.607, 95% CI: 0.34-0.86, P =0.398) at 24 hours after transplantation. Multivariate analysis revealed that only serum NGAL was significant independent predictor of DGF (OR: 0.996, 95% CI: 0.993-1.000, P =0.039). Conclusion Serum NGAL at early hours of post-transplantation was valuable biomarker for early accurate predictor of DGF in kidney transplantation compare with tradition biomarkers such as serum Cr and urine output.
Development and Validation of a Comprehensive Multiparameter-based Scoring System to Assess Pulmonary Fibrosis Severity
Authors: Yin Chengsheng , Zhang Yuan , Su Yiliang , Zhang Feng , Shi Jingyun , Chen Yanan , Weng Dong , Chen Tao , Zhao Mengmeng , Zhou Ying , Jiang Xing , Zhang Aihong , Li Huiping 
Published: February 2020

Abstract
Background Survival time varies greatly in patients with idiopathic pulmonary fibrosis (IPF). An assessment method that can accurately assess the severity and prognosis of idiopathic pulmonary fibrosis is currently lacking. This study aimed to develop a new method, which can be easily used to assess pulmonary fibrosis severity. Method 1. Development of a HRCT combined pulmonary function & physiological parameter (CTPF) assessment method: The method included two parts. 1) CT-based fibrosis staging: Four representative lung CT sections were selected and evenly divided into 100 small areas. The percentage of honeycomb lesion area in the four sections was determined fibrosis stage,2) PF-based severity grade: FVC%pred,DLco%pred,SpO2% age and gender were used to assess PF severity grade. 2. Validation of the new method: The method was used to assess 192 patients with IPF. Two radiologists used the CT-based fibrosis staging method to determine the fibrosis stage. Pulmonologist determined the PF severity grade. 3. Statistical analyses: Intra-group correlation coefficient to estimate the consistency between the CT scores from the two radiologists. Spearman correlation coefficient to evaluate the correlation between CT scores and lung function parameters. The competitive risk Fine–Gray model was used to analyze the relationship between CT-based stage/PF-based grade and prognosis. CT-based stage, PF-based grade, and GAP stage were used as predictors to predicted the death risk. Results 1. The intra-group correlation coefficient of the CT scores of the two radiologists was 0.95, P<0.05. 2. The CT scores negatively correlated with pulmonary function. 3. The CTPF comprehensive model, showed higher predictive accuracy. Conclusion Combined CT-based staging and PF-based grading methods CTPF can be adopted easily in clinical practice, and can assess IPF severity and predict death risk more accurately.
What have we learnt from measles outbreaks in 3 English cities? A qualitative exploration of factors influencing vaccination uptake in Romanian and Roma Romanian communities
Authors: Bell Sadie , Saliba Vanessa , Ramsay Mary , Mounier-Jack Sandra 
Published: February 2020

Abstract
Background: Since 2016, large scale measles outbreaks have heavily affected countries across Europe. In England, laboratory confirmed measles cases increased almost four-fold between 2017 and 2018, from 259 to 966 cases. Several of the 2017-18 measles outbreaks in England particularly affected Romanian and Roma Romanian communities, with the first outbreaks in these communities occurring in Birmingham, Leeds and Liverpool. This study explored factors influencing vaccination behaviours amongst Romanian and Roma Romanian communities in these three cities. Methods: Across Birmingham, Leeds and Liverpool, we conducted semi-structured interviews with 33 key providers to explore their experience in delivering vaccinations and managing the outbreak response. We also interviewed 9 Romanian women in one of the cities to explore their vaccination attitudes and behaviours. To categorise factors affecting vaccination we applied the 5As Taxonomy for Determinants of Vaccine Uptake (Access, Affordability, Awareness, Acceptance and Activation) during data analysis. Findings: Factors related to access and acceptance, such as language and literacy, ease of registering with a general practice, and trust in health services, were reported as the main barriers to vaccination amongst the communities. Concerns around vaccination safety and importance were reported but these appeared to be less dominant contributing factors to vaccination uptake. The active decline of vaccinations amongst interviewed community members was linked to distrust in healthcare services, which were partly rooted in negative experiences of healthcare in Romania and the UK. Conclusion: Access and acceptance, dominant barriers to vaccination, can be improved through the building of trust with communities. To establish trust providers must find ways to connect with and develop a greater understanding of the communities they serve. To achieve this, cultural and linguistic barriers need to be addressed. Better provider-service user relationships are crucial to reducing vaccination inequalities and tackling broader disparities in health service access.
Effective Data Transmission Based on Path Prediction in Opportunistic Social Networks
Authors: ZHANG Xuan , WU Jia , YU Genghua 
Published: February 2020

Abstract
With the development of social media, social networks have become an important platform for people to share and communicate. In social network communication, each participant can only pass information correctly if we find the goal of our communication. In other words, when people carry mobile devices for data transmission, they need to find a definite transmission destination to ensure the normal conduct of information exchange activities. In social networks, this is manifested in the process of data transmission by nodes, which requires analysis and judgment of surrounding areas, and finds suitable nodes for effective data classification and transmission. However, the node cache space in social networks is limited, and the process of waiting for the target node will cause end-to-end transmission delay. In order to improve such a transmission environment, this paper proposes a node trajectory prediction method named EDPPM algorithm. This algorithm can guarantee that nodes with high probability are given priority to obtain data information, which realized an effective data transmission mechanism. Through experiments and comparison of opportunistic transmission algorithms in social networks, such as Epidemic algorithm, Spray and Wait algorithm, and PRoPHET algorithm, our algorithm can improve the cache utilization of nodes, reduce data transmission delay, and improve the overall network efficiency.