bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of two commercial and two non-commercial immunoassays for the detection of prior infection to SARS-CoV-2
Authors: Nilles Eric J. , Karlson Elizabeth W. , Norman Maia , Gilboa Tal , Fischinger Stephanie , Atyeo Caroline , Zhou Guohai , Bennett Christopher L. , Tolan Nicole V. , Oganezova Karina , Walt David R. , Alter Galit , Simmons Daimon P. , Schur Peter , Jarolim Petr , Baden Lindsey R. 
Published: June 2020

AbstractBackgroundSeroepidemiology is an important tool to characterize the epidemiology and immunobiology of SARS-CoV-2 but many immunoassays have not been externally validated raising questions about reliability of study findings. To ensure meaningful data, particularly in a low seroprevalence population, assays need to be rigorously characterized with high specificity.MethodsWe evaluated two commercial (Roche Diagnostics and Epitope Diagnostics IgM/IgG) and two non-commercial (Simoa and Ragon/MGH IgG) immunoassays against 68 confirmed positive and 232 pre-pandemic negative controls. Sensitivity was stratified by time from symptom onset. The Simoa multiplex assay applied three pre-defined algorithm models to determine sample result.ResultsThe Roche and Ragon/MGH IgG assays each registered 1/232 false positive, the primary Simoa model registered 2/232 false positives, and the Epitope registered 2/230 and 3/230 false positives for the IgG and IgM assays respectively. Sensitivity >21 days post symptom-onset was 100% for all assays except Epitope IgM, but lower and/or with greater variability between assays for samples collected 9-14 days (67-100%) and 15-21 days (69-100%) post-symptom onset. The Simoa and Epitope IgG assays demonstrated excellent sensitivity earlier in the disease course. The Roche and Ragon/MGH assays were less sensitive during early disease, particularly among immunosuppressed individuals.ConclusionsThe Epitope IgG demonstrated good sensitivity and specificity. The Roche and Ragon/MGH IgG assays registered rare false positives with lower early sensitivity. The Simoa assay primary model had excellent sensitivity and few false positives.SummarySARS-CoV-2 immunoassays can be valuable tools for informing the global response, but many currently available assays have not been independently validated. We conducted a performance assessment of four assays including the Roche Diagnostics and Epitope Diagnostics immunoassays.
Cerebellar nuclei evolved by repeatedly duplicating a conserved cell type set
Authors: Kebschull Justus M , Ringach Noam , Richman Ethan B , Friedmann Drew , Kolluru Sai Saroja , Jones Robert C , Allen William E , Wang Ying , Zhou Huaijun , Cho Seung Woo , Chang Howard Y , Deisseroth Karl , Quake Stephen R , Luo Liqun 
Published: June 2020

AbstractHow have complex brains evolved from simple circuits? Here we investigated brain region evolution at cell type resolution in the cerebellar nuclei (CN), the output structures of the cerebellum. Using single-nucleus RNA sequencing in mice, chickens, and humans, as well as STARmap spatial transcriptomic analysis and whole-CNS projection tracing in mice, we identified a conserved cell type set containing two classes of region-specific excitatory neurons and three classes of region-invariant inhibitory neurons. This set constitutes an archetypal CN that was repeatedly duplicated to form new regions. Interestingly, the excitatory cell class that preferentially funnels information to lateral frontal cortices in mice becomes predominant in the massively expanded human Lateral CN. Our data provide the first characterization of CN transcriptomic cell types in three species and suggest a model of brain region evolution by duplication and divergence of entire cell type sets.
MeCP2 gates spatial learning-induced alternative splicing events in the mouse hippocampus
Authors: Brito David V.C. , Karaca Kubra Gulmez , Oliveira Ana M.M. 
Published: June 2020

AbstractLong-term memory formation is supported by functional and structural changes of neuronal networks, which rely onde novogene transcription and protein synthesis. The modulation of the neuronal transcriptome in response to learning depends on transcriptional and post-transcriptional mechanisms. DNA methylation writers and readers regulate the activity-dependent genomic program required for memory consolidation. The most abundant DNA methylation reader, the Methyl CpG binding domain protein 2 (MeCP2), has been shown to regulate alternative splicing, but whether it establishes splicing events important for memory consolidation has not been investigated. In this study, we identified the alternative splicing profile of the mouse hippocampus in basal conditions and after a spatial learning experience, and investigated the requirement of MeCP2 for these processes. We observed that spatial learning triggers a wide-range of alternative splicing events in transcripts associated with structural remodeling and that virus-mediated knockdown of MeCP2 impairs learning-dependent post-transcriptional responses of mature hippocampal neurons. Furthermore, we found that MeCP2 preferentially affected the splicing modalities intron retention and exon skipping and guided the alternative splicing of distinct set of genes in baseline conditions and after learning. Lastly, comparative analysis of the MeCP2-regulated transcriptome with the alternatively spliced mRNA pool, revealed that MeCP2 disruption alters the relative abundance of alternatively spliced isoforms without affecting the overall mRNA levels. Overall our findings reveal that adult hippocampal MeCP2 is required to finetune alternative splicing events in basal conditions, as well as in response to spatial learning. This study provides new insight into how MeCP2 regulates brain function, particularly cognitive abilities, and sheds light onto the pathophysiological mechanisms of Rett syndrome, that is characterized by intellectual disability and caused by mutations in theMecp2gene.
Repositioning the customer support services: the next frontier of competitive advantage
Authors: Sheth Jagdish , Jain Varsha , Ambika Anupama 
Published: June 2020

Purpose
This paper aims to analyze the present status of customer support services (CSS) and advocate the re-positioning of support services from an administrative cost center to a strategic profit center. Authors demonstrate how customer support or after sales services can be a source of competitive advantage and revenue generation for firms.

Design/methodology/approach
The study adopts a conceptual approach grounded in theoretical foundations of service dominant logic, customer loyalty and customer centricity along with practical illustrations from the industry.

Findings
Following the tenets of theory, review of existing research and analysis of the industry practices, the authors propose a new framework to enable the repositioning of customer service function. The key propositions include establishing customer support as separate business unit and insights center, introducing a new role of a C-level chief customer support officer to lead the customer support unit, adopting a customer-centric culture and process, enabling frontline IT support and investing in frontline employee skills development.

Research limitations/implications
Academics should examine the potential of customer support, where the strategic importance is low at present, leading to customer dissatisfaction. The new approach and positioning of customer support calls for a new direction for research in this area focusing on enablers, challenges and further implications. To succeed in this competitive era, firms should be conscious of the value of customer service and undertake concrete actions to generate value for all stakeholders.

Practical implications
Industry can use the new framework and re-position CSS of the organizations. The CSS unit can be different from other business units in the organizations. The CSS would evolve and emerge from the live customer insights. CSS unit can be managed by the C level chief CSS officer. Customer-centric culture would be developed and front line processes can be made customer-oriented by the officer. Thus, this paper and framework would provide new customer-centric directions to the organizations for effective functioning.

Originality/value
This is the original piece that has emerged from the experience and expertise of the authors.
Ability-based emotional intelligence and career adaptability: role in job-search success of university students
Authors: Mittal Shashank 
Published: June 2020

PurposeThis study aims to investigate the specific role of the components of ability-based emotional intelligence (their relative importance) in building different aspects of career adaptabilities and job-search success of university students.Design/methodology/approachThis study employed survey data from 729 full-time students enrolled in an Indian university. Hierarchical regression analyses were conducted to test the hypotheses, and the size of indirect effect was tested using SPSS PROCESS macro.FindingsThe ability-based emotional intelligence, along with the use and regulation of emotion in job-search success, plays a significant role in shaping career adaptabilities and job-search success. The ability to use and regulate emotions does have its impact on job-search success through a self-regulatory psychological resource of control and confidence over one's career. Self-emotional appraisal is necessary for an individual to be concerned for a career which forms the initiation of any job-search.Research limitations/implicationsAbility-based approach of enhancing emotional intelligence allows the university students to take a developmental approach in employment. This approach benefits the more “targeted approach to training interventions” provided by various stakeholders in the university, associated with career and employment.Originality/valueFurther, the study focuses on the psychological difficulties (over operational) faced by students in their employment endeavour. Both emotions and psychological resources are believed to play an important role in the career intervention. For instance, past researches have studied trait-based emotional intelligence as a personality construct. However, this study considers emotional intelligence as an ability-based aspect of intelligence, which “readily lends itself to interventions that can be enhanced through targeted training, coaching or counselling”.
Understanding retail quality of sporting goods stores: a text mining approach
Authors: Mao Luke Lunhua 
Published: June 2020

PurposeSporting goods retailing is a significant sector within the sport industry with the total revenue of this sector reaching $52.2 billion in 2018. Beset with formidable competition, sporting goods stores are compelled to augment their merchandise with service and improve retail quality. The purpose of this study is to investigate retail quality of sporting goods stores (RQSGS).Design/methodology/approachBased on 27,793 online reviews of 1481 stores in the United States, this study used Leximancer 4.0, a text mining software, to identify critical retail quality dimensions associated with sporting goods stores, and further explored the most salient dimensions among different levels of ratings.FindingsCustomer service and store aspects are the two higher-order dimensions of RQSGS; holistic experience, manager and staff are three themes under customer service, and product, B&M store and online–offline integration are three themes under store aspects. Furthermore, extreme reviews focus more on customer service, whereas lukewarm reviews focus more on store aspects.Practical implicationsKnowledgeable staff, managers and online–offline integration are instrumental in creating superior retail quality. Sporting goods stores should enhance hedonic and social values for consumers in order to ward off online competitions.Originality/valueThis study explored retail quality dimensions that are pertinent to sporting goods retailing utilizing text mining methods. This study to certain extent cross-validated the existing retailing literature that is developed on alternative methods.
A research-based approach for evaluating resources for transitioning to teaching online
Authors: Aguilar Stephen J. 
Published: June 2020

Purpose
This paper provides a research-based approach for evaluating resources for transitioning to teaching online.

Design/methodology/approach
This paper uses Davies’ (2011) discussion of technological literacy; Koehler and Mishra’s (2009) Technology, Pedagogy and Content Knowledge (TPACK); Leacock and Nesbit’s (2011) Learning Object Review Instrument; and Reynolds and Leeder’s (2018) expanded notion of “technology stewardship” to underpin an approach that educators can use to evaluate educational resources for transitioning to teaching online.

Findings
This paper introduces and applies an approach focused on evaluating the source of a given educational resource, as well as how it can be implemented.

Research limitations/implications
This paper synthesizes frameworks relating to qualities of educational technologies and frameworks relating to qualities of educators, and introduces two criteria for evaluating resources for transitioning to distance learning.

Practical implications
This paper provides readily applicable criteria for evaluating resources in a time of emergency distance learning.

Social implications
This approach enables educators to evaluate resources in a time of emergency distance learning.

Originality/value
The synthesis of four approaches to evaluating educational technologies, and applying the approach to four resources that have emerged to address COVID-19-related instructional needs.
Influence of independent directors on the market risks of Indian banks
Authors: Shukla Ankur , Narayanasamy Sivasankaran , Ayyalusamy Kanagaraj , Pandya Saurabh Kaushik 
Published: June 2020

PurposeThe purpose of this paper is to empirically explore the influence of independent directors (non-executive directors) on the market risks of the Indian banks.Design/methodology/approachThis paper is based on the data collected over a period of seven years (2009-2016) for a set of 29 Indian banks that are the constituents of the National Stock Exchange 500 Index. The data for independent directors of the sample banks are extracted from the annual reports of the banks, whereas the data relating to the dependent and control variables are compiled from the Ace equity and the Reserve Bank of India databases. The study uses the panel data method for analysis of the collected data for the sample banks.FindingsThis study concludes that independent directors increase the market risks for Indian banks (measured through equity beta).Originality/valueThis is, perhaps, the first paper to look into the impact of independent directors on the market risks of Indian banks. The policymakers and banks may need to be aware of the risk implications of the findings of the study in the Indian context, such that the independent directors enable their banks in reducing the market risks.
The impact of using a cloud supply chain on organizational performance
Authors: Lin Meichun , Lin Chinho , Chang Yong-Sheng 
Published: June 2020

PurposeThis study aims to indicate the advantages of using cloud computing services, including the ways in which firms use cloud-based services to achieve accessibility, better communication, flexibility and effective provision of services. However, little evidence has been obtained related to the effectiveness of applying cloud computing services to supply management chains.Design/methodology/approachIn this study, a sample of 223 top 1,000 manufacturing firms in Taiwan that had implemented cloud-based supply chain management systems (CSCMs) was surveyed to determine what kind of internal resources in these companies were allocated to this implementation effort, how collaborative relationships were established in the existing supply chain to help make the transitions successful, how well their systems are working now that they have been implemented and whether these new systems have improved cycle time performance and the overall performance of their organizations. The study also examines the interrelationships among these variables.FindingsThe results reveal that, from the perspective of the managers who were surveyed, an effective allocation of internal organizational resources does have a positive, strong effect on external CSCM conditions. They also showed that when the relationship between internal and external resources is well constructed, the result is that CSCM improves supply chain management cycle time performance, which, in turn, leads to positive organizational performance.Originality/valueThe study reports some useful insights from the managers of CSCM systems related to how the execution of CSCM solutions can improve cycle time and organizational performance by enhancing internal organizational management and joint collaboration among supply chain partners. The findings from this survey will be useful to managers who are considering creating cloud-based supply management systems in the future.
OECD’s global principles and EU’s tax crime measures
Authors: Turksen Umut , Abukari Adam 
Published: June 2020

Purpose
This paper aims to investigate and provide pathways for leveraging the Organisation for Economic Cooperation and Development (OECD’s) Ten Global Principles (TGPs) for countering tax crimes in the EU.

Design/methodology/approach
The study is guided by the combination of traditional and innovative research methods drawn from criminal law and justice, public regulatory theory and tax law, based on socio-legal and comparative methodologies.

Findings
The research shows that EU has achieved considerable amount of progress when it comes to meeting the TGPs. However, law and practice in EU Member States indicate that there are different legal, human and organisational approaches to fighting tax crimes. The TGPs could be strategically applied to complementing the EU’s Fifth Anti-Money Laundering Directive (AMLD) and other initiatives on Administrative Cooperation.

Research limitations/implications
Although the TGPs appear encompassing, there are opportunities to harness the potency of these principles and to provide more tailored principles that can help engineer sustainable remedies for countering tax crimes in the EU.

Practical implications
The paper critically analyses, through a multidisciplinary approach, the main legal, human and organisational factors influencing the prosecution of tax crimes in the EU Member States.

Social implications
Realignment and harmonisation of tax enforcement paractices in the EU Member States thus help in the reduction of tax gap resulting from tax offences.

Originality/value
The paper provides novel approaches and findings based on empirical info obtained from face-to-face focus groups with end users and law enforcement agencies in tax enforcement eco-system in ten different EU Member States.
Case management service quality and patient-centered care
Authors: Perera Sandun , Dabney Beverly Waller 
Published: June 2020

PurposeProviding care that is patient-centered is an important objective in the modern healthcare industry. Despite this objective, hospital inpatient case managers and the services they provide are evaluated routinely without including patients' perspectives. Therefore, the purpose of this study is to fill this research gap by using patient expectations and perceptions to assess the overall quality of and patient satisfaction with hospital case management services.Design/methodology/approachThis paper investigates five dimensions of case management services – reliability, responsiveness, assurance, empathy and tangibles – and how they affect overall quality and patient satisfaction. Study surveys are based on the SERVQUAL instrument. Survey data from a cross-sectional sample of 67 inpatients are analyzed using principal component analysis, confirmatory factor analysis, GAP analysis and a predictive model.FindingsThe preliminary part of the study identifies “tangibles” and “nontangibles” – reliability, responsiveness, assurance and empathy – as the main components. Among these two components, only nontangibles have a positive and significant effect on both quality and patient satisfaction according to patient perspectives. GAP analysis indicates that gaps between patient expectations and perceptions of reliability and assurance are significant. Finally, the proposed predictive model reveals that gaps in assurance have a significant impact on both overall quality and satisfaction, while gaps in empathy have a significant impact on satisfaction, but not overall quality.Originality/valueStudies on service quality at the case manager level are limited. This study is the first in this domain to evaluate quality and satisfaction from the patient perspective.
Implications of customer participation in outsourcing non-core services to third parties
Authors: De Pourcq Kaat , Verleye Katrien , Larivière Bart , Trybou Jeroen , Gemmel Paul 
Published: June 2020

PurposeFocal service providers increasingly involve customers in the decision-making about outsourcing parts of the service delivery process to third parties. The present study investigates how customers' outsourcing decisions affect the formation of the waiting experience with the focal service provider, by which the objective waiting time, environmental quality and interactional quality act as focal drivers.Design/methodology/approachTo test our hypotheses in the context of cancer care, we gathered process data and experience data by means of a patient observation template (n = 640) and a patient survey (n = 487). The combined data (n = 377) were analyzed using Bayesian models.FindingsThis study shows that opting for a service triad (i.e. outsourcing non-core services to a third party) deduces customers' attention away from the objective waiting time with the focal service provider but not from the environmental and interactional quality offered by the focal service provider. When the type of service triad coordination is considered, we observe similar effects for a focal service provider-coordinated service triad while in a customer-coordinated service triad the interactional quality is the sole experience driver of waiting experiences that remains significant.Originality/valueBy investigating the implications of customer participation in the decision-making about outsourcing parts of the service delivery process to third parties, this research contributes to the service design, service triad and service operations literature. Specifically, this study shows that customer outsourcing decisions impact waiting experience formation with the focal service provider.