bluuebunny/crossref_metadata_2025_split huggingface dataset:
A System and Method for improving the Model Based Systems Engineering Environment capability
Authors: Prasanna Kumar R N , Patil Shailesh 
Published: December 2019

AbstractAs the evolution of new technologies such as IOT and Cloud Computational Analysis continue, the Systems have become more and more complex and it is grown much in size. Unfortunately the present day SE Environments, Modeling languages and the related tool sets are not sufficient enough to represent the multiple streams problem at hand in one integrated modeling environment which makes all of the Stakeholders and Engineers & Non‐engineers understand the problem scenarios. This lack of integrated modelling environment and a methodology for an additional representation of multiple problem domains limits off the analysis capabilities, timely communication of the technical and project risks to various stakeholders for decision making. It happens due to the inherent capability & limitations of each modeling languages followed so far by the Model Based System Engineering (MBSE) community & to bridge across them. This research paper focuses on improving the SE modeling environment capability from at least three distinct directions. First, it proposes a way to strengthen the SysML semantics with concrete guidelines which helps to reduce the incompleteness & ambiguous nature of the SysML elements especially in the Non Functional Requirements modeling. Then it explores the possibility of integrating the ontology aspects offered in evolving Life‐cycle Modeling Language (LML) specification to an extended SysML profile to complement its constructs to facilitate communication with non‐system engineers as well. Finally, this paper draws parallel between the LML architectural modeling concepts with the Architecture Analysis & Design Language (AADL) constructs and realizes them in an extended SysML profile. This will facilitate the seamless flow of models at various abstraction levels & translation of architectural model information from LML to AADL analytical environment. A case study implemented using an extended SysML profile & a methodology will be presented to measure its effectiveness.
FGE‐sulfatase‐based bacterial biosensor with single copy evolved sensing cassette for arsenic detection
Authors: Sangkaew Watsawan , Sallabhan Ratiboot , Ritcharoon Benjarat , Mongkolsuk Skorn , Loprasert Suvit 
Published: December 2019

AbstractBACKGROUNDArsenic (As) is classified as a Group 1 human carcinogen, and the maximum arsenic contamination level allowed in drinking water is10 μg L−1. In this study, a genetic‐based whole‐cell biosensor for arsenic detection was constructed and engineered for rapid and more efficient arsenic detection.RESULTSThe promoter and repressor responsive to arsenic (ArsBR) were newly isolated from Bacillus cereus ATCC14579. The reporter gene was atsBA carrying formylglycine‐generating enzyme (FGE) and sulfatase, which provided a fluorescent product after reacting with 4‐methylumbelliferyl sulfate (4‐MUS). To improve the performance of the arsenic biosensor, firstly, more efficient Escherichia coli host strains were constructed, including the disruption of the arsenic efflux pump, multidrug efflux pump and arsenical‐resistant genes; secondly, a repressor (ArsBR) was engineered by an error‐prone polymerase chain reaction (PCR) technique, aiming to lower the detection limit; lastly, a single copy biosensor cassette in an E. coli chromosome was constructed.CONCLUSIONThe stable and sensitive biosensor was able to detect As(III) at 5 μg L−1 and As(V) at 50 μg L−1 in 3.5 h with a very low fluorescent background. Moreover, the roles of all seven cysteine residues of the repressor were also investigated. Interestingly, the six cysteine residues Cys 28, 34, 36, 89, 90 and 100 are functionally involved in arsenic sensing, but not Cys 101. © 2019 Society of Chemical Industry
An environmentally benign, green, and efficient ionic liquid catalyzed synthesis of Quinoline derivatives via Knoevenagel condensation
Authors: Tasqeeruddin Syed , Asiri Yahya I. 
Published: December 2019

AbstractThe present investigation is in the interest of one‐pot, environmentally friendly and efficient protocol for the synthesis of quinoline derivatives from 2‐aminoarylketones and active methylene compounds in the presence of L‐proline and ionic liquid 1,3‐dimethylimidazolium methyl sulfate [MMIm][MSO4]. The Ionic liquid which is used as a catalyst can be reused several times. The results obtained by using [MMIm][MSO4] were also compared with those described in the literature. The advantages of the method are shorter reaction time, milder conditions, easy workup and better yields. The catalyst [MMIm][MSO4] can be recovered for the subsequent reactions and reused without any appreciable loss of efficiency. This method of synthesis widens the applicability of Ionic liquids in heterocyclic compounds.
Rotator Cuff Fibro‐Adipogenic Progenitors Demonstrate Highest Concentration, Proliferative Capacity, and Adipogenic Potential Across Muscle Groups
Authors: Lee Carlin , Agha Obiajulu , Liu Mengyao , Davies Michael , Bertoy Lauren , Kim Hubert T. , Liu Xuhui , Feeley Brian T. 
Published: December 2019

ABSTRACTFatty infiltration (FI) of rotator cuff (RC) muscles is common in patients with RC tears. Studies have demonstrated that fibro‐adipogenic progenitors (FAPs), a population of resident muscle stem cells, are the main contributors of FI, which adversely affects muscle quality and RC repair success. Although FI is common in RC injuries, it is not frequently reported after other musculotendinous injuries. Additionally, studies have shown the development of different pathology patterns across muscle groups suggestive of intrinsic differences in cellular composition and behavior. This study evaluates FAP distribution and differentiation properties across anatomic locations in mice. Muscles from seven different anatomic locations were harvested from PDGFRα‐eGFP FAP reporter mice. FAPs were quantified using histology and FACS sorting with BD Aria II with CD31−/CD45−/Integrinα7−/Sca‐1+ and PDGFRα reporter signal (n = 3 per muscle). The cells were analyzed for adipogenesis using immunocytochemistry and for proliferation properties with Brdu‐Ki67 staining. In a separate group of mice, RC and tibialis anterior muscles received glycerol injection and were harvested after 2 weeks for FI quantification (n = 4). One‐way analysis of variance was used for statistical comparisons among groups, with significance at p < 0.05. FAPs from the RC, masseter, and paraspinal muscles were more numerous and demonstrated greater proliferative capacity and adipogenic potency than those from the tibialis anterior and gastrocnemius. The RC demonstrated significantly greater levels of FI than the tibialis anterior after glycerol‐injection injury. Clinical Significance: This study suggests differences in FAP distribution and differentiation characteristics may account for the propensity to develop FI in RC tears as compared with other musculotendinous injuries. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:1113‐1121, 2020
Uptake of cardiovascular health checks in community pharmacy versus general practice
Authors: Whittaker Paula J. 
Published: December 2019

AbstractThe NHS Health Check is a cardiovascular screening program for individuals aged 40–74 years that aims to identify those with early disease or at high risk of developing cardiovascular disease. Eligible patients from two matched primary care practices were offered NHS Health Checks, one group in their own general practice and the other at a local community pharmacy. The number of patients invited and number of NHS Health Checks performed was compared from 1 April 2015 to 31 March 2016 inclusive. In all, 2265 eligible patients were invited by letter to book an NHS Health Check, 1000 at a community pharmacy and 1265 at their own primary care practice. Of these, 264 people (21%) attended the primary care practice and 234 people (23%) attended a community pharmacy (p = 0.075). Offering NHS Health Checks in a community pharmacy is a viable alternative to performing checks in general practice.
Airway Surgery Communication Protocol: A Quality Initiative for Safe Performance of Jet Ventilation
Authors: Matrka Laura , Soldatova Liuba , deSilva Brad W. , Traetow Daniel 
Published: December 2019

ObjectivesTo assess whether manual jet ventilation can safely be performed with variable anesthesia and operating room (OR) staff experience levels and communication skills.MethodsJet ventilation procedures for airway stenosis at a single institution over 35 months were retrospectively reviewed. OR and anesthesia staff were assigned scores based on experience level and communication skills. Data were analyzed for any association between the experience or communication skills of the staff and the successful use of jet ventilation, complication rate, or ultimate patient outcome, controlling for intraoperative variables and patient and airway complexity. A detailed preoperative surgeon‐led communication protocol was followed in all cases.ResultsSeventy procedures in 46 patients were performed. Jet ventilation was successful in 69 of 70 cases. No relationship was found between staff experience or communication scores and the successful use of jet ventilation, complication rate, or ultimate patient outcome. The percentage of cases performed with a fully experienced team was low, at 7.1%. The experience level of the certified registered nurse anesthetist was significantly associated with likelihood of using an adequate paralytic dose upfront (P = 0.017), which in turn correlated with shorter anesthesia time by 19.7 minutes (P = 0.0131); however, neither affected complication rate nor ultimate patient outcome. The statements above remained true in cases of medically complex patients, difficult airways with high degrees of stenosis, and multiple shift changes.ConclusionsManual jet ventilation can be performed safely even in settings of lower staff experience level or communication skills given a surgeon experienced in the technique and a strict communication protocol.Level of Evidence4 Laryngoscope, 130:S1–S13, 2020
Health literacy: Impact on quality of life in head and neck cancer survivors
Authors: Nilsen Marci Lee , Moskovitz Jessica , Lyu Lingyun , Harrison Christine , Randazza Evan , Peddada Shyamal Das , Johnson Jonas T. 
Published: December 2019

ObjectivesAlthough inadequate health literacy has been shown to impact health outcomes in other cancers, little is known about its impact in head and neck cancer (HNC). This study aimed to determine the prevalence and predictors of inadequate health literacy and evaluate the association between health literacy and quality of life (QOL) in HNC survivors.MethodsWe conducted a retrospective analysis of HNC survivors evaluated in a multidisciplinary HNC survivorship clinic. Survivors had to be ≥1‐year postcompletion of treatment to be included in the analysis. Health literacy was assessed via self‐report with the Brief Health Literacy Screen (score <10 indicating inadequate health literacy), and QOL was measured using the University of Washington QOL questionnaire. Linear regression with robust standard errors was utilized to evaluate the association between health literacy and QOL.ResultsOf the 218 survivors evaluated, 13.8% (n = 30) demonstrated inadequate health literacy. After adjusting for age, marital status, site, stage, treatment modality, and years since treatment completion, social–emotional QOL scores for survivors with adequate health literacy were estimated to be 10.67 points higher than those with inadequate health literacy (P = .013). Health literacy was not significantly associated with physical QOL after adjusting for covariates (P = .130).ConclusionInadequate health literacy is associated with a lower social QOL in HNC survivors, and among those with inadequate health literacy, interventions to ameliorate the impact on QOL are needed.Level of Evidence4 Laryngoscope, 130:2354–2359, 2020
Scalp advancement for transgender women: Closing the gap
Authors: Garcia‐Rodriguez Laura , Thain Lisa McLuckie , Spiegel Jeffrey H. 
Published: December 2019

Objectives/HypothesisHairline advancement is a powerful procedure to shorten the forehead and eliminate areas of hair loss, especially along the temporal recesses. This study was undertaken to report the typical hairline advancement possible with the senior author's method for scalp advancement at the time of forehead feminization.Study DesignRetrospective chart review.MethodsA review was conducted of 29 consecutive cases that met inclusion criteria from January to March 2018 at an academic medical center. Sex, gender, age, whether galeatomy was performed, and additional variables were recorded. The distance of hairline advancement was measured in the midline and at the left and right midpupillary line. Galeatomies and rapid intraoperative tissue expansion were performed as per standard surgical protocol.ResultsFor the group of 29 individuals who underwent the specified method for scalp advancement, the average differences for the glabella to the trichion, the right midpupillary line, and the left midpupillary line were 1.90 cm, 2.07 cm, and 2.07 cm, respectively. These differences between the pre‐ and post‐observations were statistically significant at the 0.1% level (P < .001). The overall average difference in scalp advancement was 2.01 cm (also significantly different pre‐ and post‐measurements at the 0.1% level; P < .001).ConclusionsOur technique of hairline advancement provides a significant improvement in the hairline in patients seeking out advancement due to an elongated forehead while undergoing facial feminization.Level of Evidence4 Laryngoscope, 130:1431–1435, 2020
Gender Trends in Authorship of Original Otolaryngology Publications: A Fifteen‐Year Perspective
Authors: Arrighi‐Allisan Annie E. , Shukla Devki C. , Meyer Annika M. , Kidwai Sarah M. , Barazani Sharon H. , Cosetti Maura K. , Teng Marita S. 
Published: December 2019

ObjectiveTo examine trends in female author representation within original otolaryngology research between 2000 and 2015.MethodsOriginal research articles published in 11 otolaryngology journals were analyzed for 2000, 2003, 2006, 2009, 2012, and 2015. The genders of the first and last authors for each article were recorded. Overall female authorship was calculated by summing the numbers of the first, last, and both first and last female‐authored articles. Student t test and Cochran‐Armitage trend test were utilized to determine significance between years and groups.ResultsOf the 9,623 research articles published during 5 representative years, 223 were excluded due to one or more gender‐indeterminate authors. Female first authorship exhibited a significant upward trend from 2000 to 2015 (P < 0.0001), as did the proportion of literature with female first and last authors (P < 0.0001). Although female senior authorship in literature with an impact factor (IF) greater than 2 did not increase significantly (10.0% in 2000 to 10.1% in 2015; P = 0.738), this metric did increase significantly just within journals with an IF between 1 and 2 (9.7%–12.3%, P = 0.036). The proportion of articles with a female author in the first, last, or both positions increased from 28% to 39% (P < 0.0001).ConclusionIncreasing female representation in otolaryngology literature may reflect the rising proportion of women within otolaryngology as well as greater mentorship availability. Despite these auspicious strides, female‐authored articles nonetheless represent a smaller proportion of the literature, and female senior authors remain a stark minority. Future studies should identify the barriers to female access and advancement within the field.Level of EvidenceIII Laryngoscope, 130:2126–2132, 2020
Effect of systemic lupus erythematosus and rheumatoid arthritis on sudden sensorineural hearing loss
Authors: Xie Shaobing , Ning Hongting , She Yongchuan , Jing Qiancheng , Jiang Qian , Zhang Yanni , Mei Lingyun , Feng Yong , Wu Xuewen 
Published: December 2019

ObjectivesRecent reports have identified autoimmune systemic diseases as a significant risk factor for sudden sensorineural hearing loss (SSNHL). We investigated whether systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) were correlated with the hearing recovery of SSNHL.Study DesignA retrospective study.MethodsRecords of 663 SSNHL patients between January 2008 and January 2019 were retrospectively reviewed, including demographic, comorbid diseases, and hearing recovery. Patients were divided into four groups (complete, partial, slight, and no recovery) according to Siegel's criteria and Chinese Medical Association of Otolaryngology (CMAO) criteria. Multinomial logistic regression was performed to evaluate the effects of onset of treatment, initial hearing threshold, audiogram pattern, diabetes mellitus, SLE, and RA on the prognosis of SSNHL according to both criteria.ResultsPatients in complete recovery, partial recovery, slight recovery, and no improvement were 95 (14.3%), 183 (27.6%), 170 (25.6%), and 215 (32.4%) by Siegel's criteria, and 90 (13.6%), 152 (22.9%), 188 (28.4%), and 233 (35.1%) by CMAO criteria, respectively. Among the four groups, onset of treatment, initial hearing threshold, diabetes mellitus, SLE, RA, and profound audiogram pattern were found to be associated with recovery outcome by both Siegel's criteria and CMAO criteria (P < .05). According to analysis results, presence of SLE, RA, diabetes mellitus and higher initial hearing threshold were significantly correlated with a poor prognosis by both Siegel's criteria and CMAO criteria (P < .05).ConclusionComorbid SLE or RA may negatively affect the prognosis of SSNHL. Lower initial hearing threshold and absence of diabetes mellitus are associated with favorable hearing recovery.Level of Evidence4. Laryngoscope, 130:2475–2480, 2020
PLLA/POSS Nanofibers Loaded with Multitargeted pANG Composite Nanoparticles for Promotion of Vascularization in Shear Flow
Authors: Li Wenqiang , Huang Lin , Zhang Jiehong , Lin Beiman , Huang Zhiguan , Li Yuhe 
Published: December 2019

AbstractIn vitro prevascularization is particularly important for the clinical application of tissue engineering scaffolds that require vascularization. The principal challenge is simulating the dynamic in vivo environment to promote the continuous growth of blood vessels. In this study, two targeting polypeptides are linked to the two ends of an amphiphilic block copolymer, polyethyleneimine‐b‐poly(lactide‐co‐3(S)‐methyl‐morpholine‐2,5‐dione)‐b‐polyethyleneimine (PEI‐PLMD‐PEI), and self‐assembled to form positively charged nanoparticles (NPs), which can bind to negatively charged pANG through electrostatic interactions; the polypeptides are finally loaded into PLLA/polyhedral oligomeric silsesquioxane (POSS) porous fibers to prepare untargeted nanofibers (unTFs), targeted porous nanofibers (TFBs), and targeted nanofiber bundles. The effects of the porous nanofibers on human umbilical vein endothelial cell (HUVEC) transfection, spreading, proliferation, morphology, and expression of related factors are investigated under the action of shear flow force. The results show that the PLLA/POSS nanofibers can maintain stable release of multitargeted NPs for nearly 45 days. Both the dual‐targeted porous NPs and shear flow improve the pANG transfection efficiency and promote cell proliferation, and they have a good synergistic effect. These results provide a potential strategy for designing HUVEC‐specific gene carriers and using shear flow to enhance endothelialization.
Whole‐genome comparison between the type strain of Halobacterium salinarum (DSM 3754T) and the laboratory strains R1 and NRC‐1
Authors: Pfeiffer Friedhelm , Losensky Gerald , Marchfelder Anita , Habermann Bianca , Dyall‐Smith Mike 
Published: December 2019

AbstractHalobacterium salinarum is an extremely halophilic archaeon that is widely distributed in hypersaline environments and was originally isolated as a spoilage organism of salted fish and hides. The type strain 91‐R6 (DSM 3754T) has seldom been studied and its genome sequence has only recently been determined by our group. The exact relationship between the type strain and two widely used model strains, NRC‐1 and R1, has not been described before. The genome of Hbt. salinarum strain 91‐R6 consists of a chromosome (2.17 Mb) and two large plasmids (148 and 102 kb, with 39,230 bp being duplicated). Cytosine residues are methylated (m4C) within CTAG motifs. The genomes of type and laboratory strains are closely related, their chromosomes sharing average nucleotide identity (ANIb) values of 98% and in silico DNA–DNA hybridization (DDH) values of 95%. The chromosomes are completely colinear, do not show genome rearrangement, and matching segments show <1% sequence difference. Among the strain‐specific sequences are three large chromosomal replacement regions (>10 kb). The well‐studied AT‐rich island (61 kb) of the laboratory strains is replaced by a distinct AT‐rich sequence (47 kb) in 91‐R6. Another large replacement (91‐R6: 78 kb, R1: 44 kb) codes for distinct homologs of proteins involved in motility and N‐glycosylation. Most (107 kb) of plasmid pHSAL1 (91‐R6) is very closely related to part of plasmid pHS3 (R1) and codes for essential genes (e.g. arginine‐tRNA ligase and the pyrimidine biosynthesis enzyme aspartate carbamoyltransferase). Part of pHS3 (42.5 kb total) is closely related to the largest strain‐specific sequence (164 kb) in the type strain chromosome. Genome sequencing unraveled the close relationship between the Hbt. salinarum type strain and two well‐studied laboratory strains at the DNA and protein levels. Although an independent isolate, the type strain shows a remarkably low evolutionary difference to the laboratory strains.
Swimming is Compromised in Parkinson's Disease Patients
Authors: Neves Maria Ana , Bouça‐Machado Raquel , Guerreiro Daniela , Caniça Verónica , Pona‐Ferreira Filipa , Ferreira Joaquim J. 
Published: December 2019

AbstractBackgroundA recent survey reported a high risk of drowning in Parkinson's disease (PD) patients. This study intended to assess PD patients’ swimming ability and explore the disease‐related characteristics that may affect this.MethodsA cross‐sectional study was conducted in idiopathic PD patients. The assessment included swimming in 2 different styles and the evaluation of isolated technical gestures. The primary outcome was the frequency of patients who were able to swim. Limb coordination, blockage episodes, and capacity to maintain the body in a horizontal position were also evaluated.ResultsThirteen patients were evaluated. Three patients were able to swim according to the predefined definition. The inability to maintain the horizontal position and floatability were the main reasons identified for the decrease in swimming performance.ConclusionsSwimming ability is compromised in some PD patients. Further studies are needed to evaluate the global frequency of swimming difficulties in PD patients and their contributing factors. © 2019 International Parkinson and Movement Disorder Society