bluuebunny/crossref_metadata_2025_split huggingface dataset:
What happens to adiponectin, resistin and apelin-12 in colorectal adenomas? A cross sectional study.
Authors: dikbas oguz , Dagli Ülkü , Ankarali Handan , Tekce Buket Kin , Yılmaz Fahri , Gonen Mustafa Sait 
Published: January 2020

Abstract
BackgroundColorectal adenomas are precancerous neoplastic lesions which may potentially differentiate to the colorectal carcinoma. We investigated whether adiponectin, resistin and apelin 12 serum levels might change in case of colorectal neoplasia. Aims In this study we intended to determine relationship between serum levels of adiponectin, resistin, apelin-12 and presence of colorectal adenoma using case-control approach. Methods Patients undergoing screening colonoscopy in the Abant Izzet Baysal University Medical Faculty Gastroenterology Polyclinics between years 2010 and 2013 were selected for study. Results In this study there were not any difference between groups according to age, body mass index, waist circumference and mean arterial blood pressure (all p>0.05). Adiponectin, resistin and apelin-12 serum levels were not statistically different between groups (p=0.642, p=0.890, p=0.618; respectively). On the other side: Serum apelin-12 levels were found to be statistically higher in patients with severe dysplastic adenoma group compared to both non-dysplastic and without adenoma groups (p=0.014). There was a negative correlation between the number of colorectal adenomas and serum adiponectin levels (p=0.035, r=-0.41). Conclusion Apelin-12 does increase in severe dysplastic adenomas. Apelin-12 is an angiogenic adipocytokine with oncogenic potential. The relation between cancer development and apelin has been shown in different types of tumors. Apelin-12 might be a candidate marker for detecting dysplastic colorectal adenomas.
Screening of marine fungal strains for laccase-like activities amenable to biotechnological applications
Authors: ALI Wissal BEN , Chaduli Delphine , Navarro David , Lechat Christian , Turbé-Doan Annick , Bertrand Emmanuel , Faulds Craig B. , Sciara Giuliano , Lesage-Meessen Laurence , Record Eric , Mechichi Tahar 
Published: January 2020

Abstract
Background Environmental pollution is one of the major problems that world is facing to date. Several approaches are been studied and oxidative enzymes from microbial organisms represent an eco-friendly and cost–effective processes, amenable to biotechnological applications, as for instance industrial dye decolorization. The aim of this study was to screen marine-derived fungal strains isolated from three coastal areas in Tunisia, to identify laccase-like activities, and to produce and characterize active fungal secretomes of interest for dye decolorization. Results Following the screening of twenty fungal strains isolated from the harbours of Sfax and Monastir (Tunisia), five strains were identified displaying laccase-like activities. Molecular based taxonomic approaches allowed us to identify these strains as belonging to the species Trichoderma asperellum , Stemphylium lucomagnoense and Aspergillus nidulans . Among these five isolates, one T. asperellum strain ( T. asperellum 1) gave the highest level of secreted oxidative activities, and as such it was chosen for further studies. Optimization of the growth medium for liquid cultures was first studied to improve the level of laccase-like activity in culture supernatants. Finally, T. asperellum 1 secretome allowed decolorizing different synthetic dyes belonging to diverse dye families, in the presence or absence of 1-hydroxybenzotriazole (HBT) as a mediator. Conclusions The optimal growth conditions to produce laccase-like active secretomes from T. asperellum 1 were 1.8 mM CuSO 4 as an inducer, 1% NaCl to mimic seawater environment and 3% sucrose as a carbon source. T. asperellum 1 secretome was effective to decolorize different synthetic dyes belonging to diverse chemical classes, and the presence of HBT as a mediator improved the decolorization process.
Grape cultivar features differentiate the grape rhizosphere of microbiota
Authors: Yang Zhiguang , Sun Bo , Zhang Shiwei , Bao Lijun , Wei Yingxue , Li Zaixing , Bai Zhihui 
Published: January 2020

Abstract
Background: Despite its importance to crop health and plant quality, the structure and diversity of the grape rhizosphere remains poorly described. In this study, the microflora community structures of grapes in northern China were compared using high-throughput sequencing. Results: We found that richness, diversity of bacterial and fungi community networking in the root compartments were significantly influenced by the grape variety. The bacterial linear discriminant analysis showed that Pseudomonas , Rhizobium which considered as potential plant-growth-promoting bacteria were more enriched in Pinot noir, and potential ammonia-oxidizing Nitrosospira was enriched in Gem. The fungal linear discriminant analysis showed that Fusarium was more enriched in Longan, Sporormiella more enriched in Merlot while Gibberella and Pseudallescheria were more enriched in Gem, and Mortierella was more abundant in Cabernet Sauvignon. Conclusions: The 16S rRNA functional prediction indicated that no significance differentiate among the varieties. The results showed that may be we can extinguish the varieties according to these characteristic bacterial and fungal genus.
The exacerbation of soil acidification correlates with structural and functional evolution of the soil microbiome upon long-term agricultural intensification
Authors: Shen Jie , Luo Youlin , Li Meng , Tao Qi , White Philip J , Cadre Edith Le , He Yuting , Li Bing , Li Qiquan , Xu Qiang , Cai Yan , Li Huanxiu , Li Yiding , Tang Xiaoyan , Guo Lingke , Zhao Junwen , Wang Changquan 
Published: January 2020

Abstract
Background: Agricultural intensification induces prolonged impacts on soil acidification (SA), whereas linking the mechanisms therein to structural and functional evolution of soil microbiota remain unclear, especially over longer time scales. Methods: To investigate the short- and long-term effects of agricultural intensification on soil microbiome and its association with SA severity, we performed metagenomic sequencing of soil samples from typical rice-vegetable rotations for 0 (control, V0), 10 (V10), 20 (V20) years. The objectives were to clarify biomarkers that indicative of SA and its putative functions of acid production. Results: Rice-vegetable rotations for 0, 10, 20 years yielded three well-defined soil clusters with differential acid saturation (V0: 4.5%; V10: 11.8%; V20: 40.7%). Soil pH declined significantly (p < 0.05) from the 6.38 (V0) to 4.82 (V20). Acid cations (H + , Al 3+ ) dominating V20’s exchangeable cation pool, suggesting that soils in V20 were highly acidic and acid-sensitive, which recruited distinct microbiomes. The increased acid cations and NO 3 - -N concentrations are driving forces that lead to a higher abundance of Rhodanobacter , Gemmatirosa , Sphingomonas and Streptomyces in V20 samples, which acting as aciduric biomarkers that significantly and positively correlated with soil acidity. Furthermore, functional modules of microbial genes revealed V20 samples contained more genes associated with“oxidative phosphorylation” and “two-component system”, particularly enriched multiple pathways of cytoplasmic pH homeostasis. More specifically, we found increased genetic abundances of active efflux of protons and cytoplasmic proton consumption in V20 samples over than in V0, which are both involved in the microbial dehydrogenation and acid production, and favoured the growth of Rhodanbacter , Gemmatirosa , Sphingomonas , Streptomyces . Conclusions: Collectively, the identified aciduric biomarkers was closely associated with SA severity in intensive agroecosystems. An overall increase with the ongoing of intensive cropping history in the abundance of indicative genes associated with dehydrogenation and acid production pathways. The differential community compositions provide new clues to elucidate the interaction between the soil microbiome and SA severity, and varying dehydrogenation and acid production pathways provide novel insights into the exacerbation of SA. Keywords: Agricultural intensification, Soil acidification, Metagenomics, Soil microbiome, pH homeostasis
The cooperative complex of Argonaute-2/MicroRNA-146a regulates Hepatitis B Virus replication through FEN1
Authors: ji min , Mei Xiaoping , Jing Xunming , Xu Xu , Chen Xing , Pan Wanlong 
Published: January 2020

Abstract
Background: The regulatory of HBV replication is still unclear. FEN1 can repair HBV rcDNA to HBV cccDNA and promote HBV DNA replication. However, its specific regulatory detail remains unclear. MicroRNA regulates gene expression at post-transcriptional level. Especially, miR-146a, it plays an important role that is closely related to regulation of HBV replication. Based on above, we hypothesize that miR-146a may be regulate HBV cccDNA formation through FEN1. So, we will investigate the effect of miR-146a on the replication of hepatitis B virus and its molecular mechanism. Results: We found that level of miR-146a was significantly up-regulated in HepG2.2.15 cells (11.755±0.069) than that in HepG2 (1.000±0.038) (P<0.05). Furthermore, HBV-DNA copies and FEN1 were significantly increased and decreased, respectively, in HepG2.2.15 cells transfected with miR-146a mimic and inhibitor for 48h,[(3.215±0.001); (2.623±0.083)] compared with the control group (2.813±0.015) (P<0.05),. After transfection FEN1 plasmid, HBV-DNA Copies (5.712±0.371) is significantly higher than the control group（2.661±0.009）(P<0.05), and the level of miR-146a（3.431±0.004）is significantly higher than the control group (1.023±0.224） (P<0.05). The expression level of IRAK1/TRAF6 are significantly lower and higher [(0.114±0.013); (0.390±0.014); (1.222±0.073); (2.145±0.271)] than the control group [(1.000±0.038); (1.007±0.119)] (P<0.05) after transfection miR-146a mimic and inhibitor into HepG2.2.15. After Ago2 siRNA, the level of miR-146a (0.105±0.002) is significantly decreased than the control group (1.000±0.041) (P<0.05) from Ago2 protein RIP. After transfection Ago2 siRNA then added into exogenous miR-146a into HepG2.2.15, The expression level of FEN1 is significantly reduced (0.485±0.100) than the control group (1.000±0.023) (P<0.05), and the HBV-DNA copies is significantly lower (3.230±0.047) than the control group (3.789±0.041) (P<0.05). Conclusion: Ago2 cooperates with miR-146a to regulate the transcription the expression level of FEN1 protein through the downstream target gene IRAK1/TRAF6, then promoting HBV replication.
Red/ET recombineering enhances extracellular lipase production by Burkholderia glumae
Authors: Tu Qiang , Bian Zhilong , Fang Qian , Chen Hanna , Xie Zhiling , Pan Deng , You Beian , Xia Liqiu , Zhang Youming , Gong Yaling 
Published: January 2020

Abstract
Background: Microbial lipases are utilized widely in industrial fields, and the LipA lipase produced by Burkholderia glumae PG1 has been used for the production of enantiopure pharmaceuticals. However, efficient lipase expression and secretion are still problematic. LipA is encoded in an operon that also contains the lipase-specific foldase gene lipB. The purpose of this study was to enhance the production of active lipase by overexpressing lipAB in B. glumae PG1 using a host-vector system.Results: The lipAB operon, isolated from the genomic DNA of B. glumae PG1, was directly cloned into broad-host-range vectors, with pBBR1 or pRk2 backbones, using the Red/ET recombination system and then transformed into the original host strain. Lipase activity of the derivative strains, including PG1/Bl and PG1/Rl, were evaluated. When 1% olive oil was included in the growth medium, the maximum activities of the resultant strains were approximately 39.5- and 15.3-fold higher, respectively, than that of the wild-type PG1, indicating that increased lipase gene dosage resulted in high expression yields of lipase. To further enhance lipase expression, two recombinant strains, PG1/Ba and PG1/Ra, were generated with the plasmid-borne lipAB operon driven by the strong constitutive promoter Papra. PG1/Ba and PG1/Ra displayed 15.0- and 4.3-fold improvements in lipase production in 0.5% glucose-containing medium compared to PG1/Bl and PG1/Rl, respectively. However, glucose repressed lipase gene expression in strain PG1 when the native lipAB promoter was used.Conclusions: Homologous expression of lipase genes in B. glumae PG1 was achieved by the use of high-copy expression vectors, and the resulting presence of multiple copies of lipAB significantly improved extracellular lipase expression. Our results demonstrate that modification of B. glumae PG1 can improve this host system for lipase production.
Thermoelasticity of tremolite amphibole: Geophysical implications
Authors: Peng Ye , Mookherjee Mainak 
Published: January 2020

AbstractWe investigated the structure, equation of state, thermodynamics, and elastic properties of tremolite amphibole [Ca2Mg5Si8O22(OH)2] up to 10 GPa and 2000 K, using first principles simulations based on density functional perturbation theory. We found that at 300 K, the pressure-volume results can be adequately described by a third-order Birch-Murnaghan equation of state with bulk moduli K0 of 78.5 and 66.3 GPa based on local density approximation (LDA) and generalized gradient approximation (GGA), respectively. We also derived its coefficients of the elastic tensor based on LDA and GGA and found that the LDA result is in good agreement with the experimental results. At 300 K, the shear modulus G0 is 58.0 GPa based on LDA. The pressure derivative of the bulk modulus K′ is 5.9, while that of the shear modulus G′ is 1.3. The second Grüneisen parameter, or δT = [–1/(αKT)](∂KT/∂T)P, is 3.3 based on LDA. We found that at ambient conditions, tremolite is elastically anisotropic with the compressional wave velocity anisotropy AVP being 34.6% and the shear wave velocity anisotropy AVS being 27.5%. At higher pressure corresponding to the thermodynamic stability of tremolite, i.e., ~3 GPa, the AVP reduces to 29.5%, whereas AVS increases to 30.8%. To evaluate whether the presence of hydrous phases such as amphibole and phlogopite could account for the observed shear wave velocity (VS) anomaly at the mid-lithospheric discontinuity (MLD), we used the thermoelasticities of tremolite (as a proxy for other amphiboles), phlogopite, and major mantle minerals to construct synthetic velocity profiles. We noted that at depths corresponding to the mid-lithosphere, the presence of 25 vol% amphibole and 1 vol% phlogopite could account for a VS reduction of 2.3%. Thus based on our thermoelasticity results on tremolite amphibole, it seems that mantle metasomatism could partly explain the MLD.
Systematics of H2 and H2O evolved from chlorites during oxidative dehydrogenation
Authors: Lempart Małgorzata , Derkowski Arkadiusz , Strączek Tomasz , Kapusta Czesław 
Published: January 2020

AbstractThermally induced dehydroxylation and oxidative dehydrogenation drive the thermal decomposition of all Fe2+-containing phyllosilicates. Whereas the former produces H2O gas, the latter results in H2 evolution. Six chlorites representing the Mg-Fe2+ series from clinochlore to chamosite and biotite (as an analog of the 2:1 layer in chlorite) were investigated using thermogravimetry coupled to quadrupole mass spectrometry (TG-MS). A fast-ramp heating protocol was applied to identify if and how hydrogen gas was released from the crystal structure and whether it was quantitatively related to structural Fe2+ content. Dehydroxylation and oxidative dehydrogenation were tested under inert and oxidizing conditions.H2 liberation confirmed the H2 gas production by oxidative dehydrogenation, as shown by an evolution of the m/z = 2 signal for chamosites, Fe-rich clinochlores, and biotite heated under nitrogen gas atmosphere. Along with H2 evolution, H2O (m/z = 18) was released, suggesting that dehydroxylation is a trigger for dehydrogenation. The higher the Fe2+ content in the studied chlorites, the more intense the H2 evolution, thus the higher the H2/H2O ratios. The products of ramp-heating to 1000 °C resulted in varying amounts of newly formed Fe3+ (from 7 to 22%), however, biotite that converted into oxybiotite underwent almost complete oxidation, indicating a stronger tendency of 2:1 layer to dehydrogenation. The observed concurrent, but independent mechanisms of H2 and H2O evolution produced a feasible model of the thermal decomposition of chlorites.Despite O2 availability under oxidizing condition, the Fe2+ oxidation was not driven by attaching oxygen anions to the phyllosilicate structure, but also by dehydrogenation. Hydrogen was not detected using MS for any tested sample heated in synthetic air because any H2 if released was instantaneously combined with external O2, which resulted in an excess H2O MS signal not matched by mass loss on the TG profiles of chamosite and biotite. Without coupling of the evolved gas analysis with TG, the excess H2O produced by dehydrogenation in the O2-bearing carrier gas would result in misleading interpretations of dehydroxylation.Methodological and geological implications of the TG-MS experiments are discussed. The oxidation of Fe2+ in all Fe2+-containing phyllosilicates proceeds with simultaneous H2 gas release that is not dependent on oxygen fugacity nor temperature during the mineral formation. Therefore, the correlation between Fe3+/Fe2+ and remaining hydrogen in the structure must be considered during modeling the conditions that involve chlorite as geothermobarometer. H2 release during heating is proposed as an indicator of oxidative dehydrogenation of Fe2+-bearing minerals on Mars.
Can model-based data products replace gauge data as input to the hydrological model?
Authors: Sivasubramaniam K. , Alfredsen K. , Rinde T. , Sæther B. 
Published: January 2020

Abstract
Hydrological models require accurate and representative meteorological inputs for better prediction of discharge and hence, the efficient management of water resources. Numerical weather prediction model-based reanalysis data products on the catchment scale are becoming available, and they could be an alternative input data for hydrological models. This study focuses on the applicability of a set of model-based data as input to hydrological models used in inflow predictions for operational hydropower production planning of three hydropower systems in middle Norway. First, the study compared the data products with gauge measurements. Then, Hydrologiska Byråns Vattenbalansavdelning (HBV) models of the three catchments were calibrated with three different meteorological datasets (model-based, gauge and observational gridded) separately using a Monte Carlo approach. It was found that the correlation between the model-based and gauged precipitation was highly variable among stations, and daily values showed a better correlation than hourly. The performance of model-based input data with daily timestep was nearly as good as the gauge or gridded data for the model calibration. Further, the annual simulated flow volume using the model-based data was satisfactory as similar to the gauge or gridded input data, which indicate that model-based data can be a potential data source for long-term operational hydropower production planning.
Energetic refurbishment of an existing large-scale MBR
Authors: Brepols C. , Drensla K. , Janot A. , Schäfer H. 
Published: January 2020

Abstract
Operations of an existing municipal MBR serving 80,000 inhabitants were improved energetically by implementing new automation routines for biology and membrane filtration. Finally, the MBR was refurbished by adding primary clarification and anaerobic sludge digestion to the treatment process, while specific energy consumption was reduced by one third to 0.65 kWh/m3 permeate produced. A combined heat and power engine generates electrical power on site from digester gas. The projected carbon footprint of plant operations will thus be halved by reducing indirect emissions from electricity consumption.
QUALIDADE DE VIDA NO TRABALHO: ESTUDO DE CASO EM UM HOTEL NA ORLA DE CABO BRANCO - JOÃO PESSOA – PB
Authors: Menezes Paula Dutra Leão de , Mello Ana Maria Nery Soares de , Felisberto Hanna Melissa Pereira , Nogueira Niedja Glória 
Published: January 2020

A insatisfação e a desmotivação são problemas que afetam trabalhadores independentemente de sua posição na empresa hoteleira. Para lidar com esses problemas torna-se necessário encontrar elementos intrínsecos e extrínsecos ao ambiente de trabalho que afetam diretamente o trabalhador. Para tanto, é fundamental encontrar a origem da insatisfação e medi-la, para que o gestor tome as medidas acertadamente. Este artigo tem como objetivo analisar a percepção dos gestores em relação a Qualidade de Vida no Trabalho (QVT). Este estudo de caso caracteriza-se por ser do tipo exploratório e qualitativo. O instrumento de coletas de dados foi um questionário fechado. A maioria dos aspectos avaliados apresentaram satisfação dos gestores. Entretanto, no aspecto satisfação sociológico/relacional sobre o quesito liberdade de expressão sem ser prejudicado quando expressa suas opiniões revelou-se insatisfatório, por sua vez, 75% dos gestores entrevistados sentem satisfação em se expressar. Sobre a satisfação psicológica/comportamental, o desenvolvimento pessoal e profissional também revela que os gestores estão insatisfeitos com o incentivo da empresa em termos de oferta de atividades e cursos relacionados a seu trabalho. Por último, registra-se a importância de observar a satisfação e motivação dos gestores considerando que são os líderes da equipe e consequentemente os motivadores dessas.
Narrativas literárias e referências de tempo e espaço em paisagens representativas do imaginário coletivo
Authors: Klein Regina Maria Martins de Araujo , Hardt Letícia Peret Antunes , Hardt Carlos 
Published: January 2020

Diante da interpretação da cidade como imagem de ações da sociedadee considerando a necessidade de compreensão do imaginário humano para aconstrução de identidades individuais e grupais, o objetivo geral desta pesquisaconsiste em analisar o contexto em que a paisagem urbana tem sido referenciada no tempo e no espaço pela memória coletiva, adotando narrativas literárias de Manhattan (Nova York, Estados Unidos) como estudo de caso. Com base em postulados teóricos sobre os temas centrais de investigação, os procedimentos metodológicos foram fundamentados em um recorte amostral de 99 narrações de publicações no intervalo temporal de 196 anos, sistematizadas pela sua localização em mapa específico daquele distrito administrativo para identificação da frequência de citação por tipologia de elemento urbanístico (caminhos, limites, regiões, pontos nodais e marcos) e de ordenamento no tempo (horizontal: continuidade; vertical: especificidade). A análise dos resultados aponta, de forma intrínseca, para a legibilidade da regiãoestudada, com especial destaque para vias públicas e, de maneira genérica, para a necessidade de aprofundamento dos estudos para a devida estruturação de subsídios aos processos de planejamento de paisagens urbanas.