bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kneeling assessment after Anterior Cruciate ligament reconstruction using Bone-Patellar Tendon-Bone autograft versus hamstring autograft
Authors: Hulaibi Fahad Al , Eid Zainab Al , Altaher Saad , Albarrak Abdulaziz , Alzaid Zaid 
Published: May 2020

Abstract
Background

ACL reconstruction surgeries is a common surgery that aim to provide stability and return functionality post ACL injury. Commonly, bone patellar tendon-bone (BPTB) and hamstring tendon autografts are used for the reconstruction. The choice of optimal autograft remains debatable to achieve satisfactory outcomes for various activities after reconstruction, including kneeling as widely needed, especially in prayers among Muslims. Kneeling with considerable ranges of motion and the minimum level of pain is still under rigorous investigations to choose proper autograft.
Purpose

Hereby, we compare kneeling outcomes post-operatively using bone-patellar tendon-bone autografts with hamstring autografts for ACL reconstruction.
Methods

A retrospective cohort study; Level of evidence (III) has enrolled. Adult patients underwent primary ACL reconstruction using either BPTB or HS autografts after they consented at a single institution with a minimum of 1-year as follow-up post-operatively were reviewed for assessment of the surgery’s outcomes in regards of kneeling pain and subjective assessment measures using International Knee Documentation Committee (IKDC) evaluation system.
Results

A total of Thirty-two males were met our inclusion criteria. Analysis of data provided that 12 patients were in the BTB group and 20 patients in the HS group. In all patients of our study, ACL rupture was sustained during participation in athletic activity regardless the type of sport with 60.8% patients were affected of left knee. After follow-up of 1-year, clinical evaluation of anterior drawer test postoperatively resulted with Two patients in HS group were positive. There was no statistically significant difference between scores of two groups when comparing patient-reported outcome measures, with mean IKDC scores of 68.5 and 62.55 in the BTB and HS groups, respectively. Interestingly, insignificant statistical difference observed in both groups in regard of kneeling pain in the BTB group when compared with the HS group.
Conclusion

The findings of our study have shown that Arthroscopic ACL reconstruction using either hamstring autograft or bone patellar tendon autograft ends with equally reasonable satisfactory outcomes, with an insignificant difference in kneeling pain between both groups.
Development of Dual Fluorescent Microsphere Immunological Assay for Detection of Pseudorabies Virus gE and gB IgG Antibodies
Authors: ji chihai , Wang Jingyu , Zeng Yuchen , Pan Haoming , Wei Yingfang , Liang Guan , Ma Jun , Gong Lang , Zhang Wei , Zhang Guihong , Wang Heng 
Published: May 2020

Abstract

Background Pseudorabies, also known as Aujezsky’s disease, is an acute viral infection caused by pseudorabies virus (PRV). Swine are one of the natural hosts of pseudorabies, therefore, the disease brings huge economic losses to the swine industry. Establishment of a differential diagnosis technique that can distinguish between wild-type infected and vaccinated pigs, and monitor vaccine-induced IgG is crucial for eventual eradication of pseudorabies.Results The aim of this study was to develop a rapid dual detection method for PRV gE and gB protein IgG antibodies with high specificity and sensitivity. PRV gE codons at amino acid residues (aa) 52–238 and gB codons at aa 539–741 were expressed to obtain recombinant PRV gE and gB proteins by pMAL-c5x vector. After purification with Qiagen Ni–NTA agarose affinity, the two proteins were analyzed by SDS-PAGE and immunoblotting assay. Two single fluorescent-microsphere immunoassays (FMIA) were established by coupling two recombinant proteins (gE and gB) with two magnetic microbeads and an effective dual FMIA was developed by integrating the two single assays. Optimal serum dilution for each assay, correlation with other common swine virus-positive sera and comparison with ELISA for two PRV antigens were tested for validation. Compared with ELISA, the specificity and sensitivity were 99.26% and 92.3% for gE IgG antibody detection and 95.74% and 96.3% for gB IgG antibody detection by dual-FMIA.Conclusion We provide a new method for monitoring PRV protective antibody in vaccinated pigs and differentiating wild-type-PRV-infected from vaccinated pigs
MiR-199a-3p/5p participate in TGF-β and EGF induced EMT in pterygium by targeting DUSP5/MAP3K11
Authors: He Siying , Huang Yifang , Dong Shiqi , Qiao Chen , Yang Guohua , Zhang Shuai , Wang Chen , Xu Yuting , Zheng Fang , Yan Ming 
Published: May 2020

Abstract
Background: Recently, it has been reported that miRNA is correlated with pterygium, however its exact mechanism in pterygium is unrevealed and require further investigation. Methods: The differential expression of miRNA in pterygium was profiled using microarray and validated with quantitative real-time PCR (qRT-PCR). Human conjunctival epithelial cells (HCEs) were cultured and treated with TGF-b and EGF. Western blot and immunohistochemistry were carried out to detect epithelial-mesenchymal transition (EMT) markers. Wound healing and transwell assay were used to determine cell migration ability, while apoptosis was determined by flow cytometry. The target genes of miR-199a were confirmed by the dual-luciferase reporter assay. Results: TGF-b and EGF induced EMT in HCEs to mimic the pathogenesis of pterygium. MiR-199a-3p and miR-199a-5p induced EMT in HCEs, whose respectively downstream targets DUSP5 and MAP3K11 hindered EMT in EMT-HCEs in turn. TGF-b and EGF induced EMT promotion and target genes suppression, could be promoted by miR-199a-3p and miR-199a-5p, while impeded by miR-199a-3p and miR-199a-5p inhibitors. The expression levels of miR-199a and target genes were further validated in pterygium tissues, which were consistent the results in cell model. Bioinformatics analysis indicated the miR-199a-3p/5p-DUSP5/MAP3K11 was belong to MAPK signalling pathway in pterygium. Conclusions: TGF-b and EGF probably induced EMT of HCEs through miR-199a-3p/5p-DUSP5/MAP3K11 axis, which explained the pathogenesis of EMT in pterygium and might provide new targets for pterygium prevention and therapy.
Global health activists’ lessons on building social movements for Health for All
Authors: Musolino Connie Marguerite , Baum Fran , Freeman Toby , Labonté Ronald , Bodini Chiara , Sanders David 
Published: May 2020

Abstract
Background
The People’s Health Movement (PHM) formed in 2000 and drew inspiration from the Alma Ata Declaration on Primary Health Care’s ‘Health for All’ (1978). Since then PHM has been an active part of a global counter-hegemonic social movement. From locations across the world including eleven countries, health activists shared their insights on social movement building, drawing on the successes and failures over many decades of striving to improve health, justice and equity.
Methods
Qualitative research methods were employed in this study to capture complex and historical narratives of individual activists, including semi-structured interviews and thematic analysis of transcripts. The research design and analysis were informed by social movement theory and literature on health activism as a pathway for social change. In this paper we examine the semi-structured interviews of 15 health activists who are part of the PHM, to derive lessons for strengthening movements for Health for All.
Results
This paper locates the activists’ narratives within a socio-political analysis of the global trends of late modern individualism and capitalist neoliberalism to understand the challenges faced by civil society groups mobilising collective action and building social movements for Health for All. This study found that within the constraints of the neoliberal socio-political and economic conditions which have caused the rise in social and health inequities, this group of long-term health activists have been nurturing alternative approaches to structuring society and building collective agency to improve health.
Conclusion
This paper draws on the practical long-term experiences of the PHM activists to understand better the processes and motivations that lead to and sustain health activism, and the dilemmas, strategies, impacts and achievements of such activism.
Effects of probiotic BaSC06 on intestinal digestion and absorption function, antioxidant capacity and macrophage polarization and microbiota composition in fattening pigs
Authors: Cao Xuefang , Tang Li , Zeng Zihan , Wang Baikui , Zhou Yuanhao , Wang Qi , Zou Peng , Li Weifen 
Published: May 2020

Abstract
Background: The previous study in our team found that supplementation of probiotic Bacillus amyloliquefaciens SC06 (BaSC06) as an alternative to antibiotics (Kitasamycin) can effectively reduce the diarrhea rate and promote the growth performance of weaned piglets. We speculate that BaSC06 may become a better substitute for antibiotics for fattening pigs. Hence, the objective of the present study is to evaluate the effects of BaSC06 substitute antibiotics on growth performance, digestive functions, antioxidant capacity, macrophage polarization and intestinal microbiota of fattening pigs. Methods: A total of 117 fattening pigs with similar weight and genetic basis were selected and divided into three groups: Anti group, fed with basal diet supplemented with 40 g/t Kitasamycin; Anti + Ba group, fed with 1 × 108 CFU/kg BaSC06 and 20 g/t Kitasamycin; Ba group, fed with 1 × 108 CFU/kg BaSC06 replaced Kitasamycin. Each treatment had three replications of 13 pigs per pen. Results: It indicated that BaSC06 replacement significantly improved ADFI and ADG in fattening pigs. It also improved intestinal digestion and absorption function with the increased activities of intestinal digestive enzymes and higher expression of glucose transporters SGLT1 and small peptide transporters PEPT1, maintained intestinal integrity significantly. Besides, supplementation with BaSC06 enhanced intestinal and body antioxidant capacity by activating Nrf2/ Keap1 antioxidant signaling pathway. In addition, BaSC06 as a substitute could alleviate intestinal inflammation by inhibiting the production of pro-inflammatory cytokines and chemokine and simultaneously increase the expression of M1 and M2 macrophage marker proteins in the intestinal mucosa. The transcription factor STAT3 regulated M2 polarization, which maintained immune homeostasis and reduced inflammation. What’s more, BaSC06 could improve the composition of the microbiota as well by increasing the proportion of Firmicutes, and reducing the proportion of Bacteroidetes and Proteobacteria. Conclusions: Taken together, our findings indicated that diet supplementation with BaSC06 instead of antibiotics increased the growth performance of piglets by improving the digestion and absorption of nutrients, intestinal microbiota composition and mucosal structure, antioxidative capacity and immune functions (including increasing M1and M2 polarization of macrophage).
Assessment of knowledge and practice of nurses working in gynecology emergency room towards pregnancy induced hypertension in selected government public hospitals found in Addis Ababa, Ethiopia
Authors: Tadele Wolelaw , Debebe Finot , Tadele Achamyelesh , Tilahun Lehulu 
Published: May 2020

Abstract
Background: - Pregnancy induced hypertension is one of the most common causes of both maternal and neonatal morbidity. Objective: - To assess knowledge and practice of nurses working in gynecology emergency room towards Pregnancy Induced Hypertension and association of having training and work experience to their knowledge and practice in selected government public hospitals of Addis Ababa, Ethiopia.Methods: - Institution based descriptive cross sectional study was conducted. Hospitals were selected by simple random sampling using lottery method with sample size determination of all nurses found in Gynecology emergency Room. Result: - A total of 78 study participants were included in the study. The mean age of the respondents was 25.62 years. Out of the total study participants, 54(67.9%) of them found to have adequate knowledge towards pregnancy induced hypertension. However, among the total 78 charts reviewed, 39(50%) of the charts had showed good practice towards pregnancy induced hypertension. Only training was significantly associated with knowledge towards pregnancy induced hypertension at p<0.003 but none of the variables were significantly associated with practice towards pregnancy induced hypertension.Conclusion: - Based on the findings of this study, the level of knowledge and practice was found to be low.
Prognostic value of lncRNA SNHG20 as a biomarker in human cancers: a systematic review and meta-analysis
Authors: Li Yongfeng , Rui Xinmiao , Chen Daobao , Xuan Haojun , Yang Hongjian , Meng Xuli 
Published: May 2020

Abstract
Background: Long noncoding RNA small nucleolar RNA host gene 20 (SNHG20) is a novel oncogene and dysregulated in a variety of human cancers. It has been revealed to be associated with the clinicopathological features and prognosis. However, the prognostic value of SNHG20 in various cancers remains unclear. Therefore, we performed this meta-analysis to evaluate the relationship between SNHG20 expression and clinical outcomes in human cancers.Methods: Comprehensive literature search was performed in PubMed, Web of Science, CNKI and Wangfang databases, and eligible studies were obtained according to the inclusion and exclusion criteria. The pooled hazard ratios (HRs) and odds ratios (ORs) were applied to assess the clinical value of SNHG20 expression for overall survival (OS) and clinicopathological features.Results: A total of 16 articles including 1190 cancer patients were included in the study. The pooled results demonstrated that evaluated SNHG20 expression was positively related to a poorer OS of cancers (HR=2.36, 95%CI: 1.85-2.87, P<0.001). Subgroup analysis revealed that SNHG20 overexpression was closely related to the low OS of patients with the digestive system cancer (HR=2.92, 95%CI: 1.96-3.88, P<0.001), sample size >80 (HR=2.42, 95%CI: 1.69-3.14, P<0.001), direct HR estimation method (HR=2.65, 95%CI: 1.78-3.52, P<0.001), and median ratio as cut-off value (HR=2.21, 95%CI: 1.60-2.83, P<0.001). In addition, the pooled data also showed that SNHG20 was positively linked to lymph node metastasis (LNM) (OR=1.65, 95%CI: 1.21-2.26, P=0.002), distant metastasis (DM) (OR=1.76, 95%CI: 1.10-2.83, P=0.02), and advanced TNM stage (OR=1.79, 95%CI: 1.34-2.39, P<0.001). Moreover, the results of the trim and fill analysis confirmed the reliability of our finding. Conclusions: Upregulation of SNHG20 was associated with advanced TNM stage, worse LNM and DM, and shorter OS, suggesting that SNHG20 may serve as a biomarker for prognosis and clinicopathological characteristics in human cancers.
Risk of depression and common comorbid mental disorders in persons with malaria: a protocol for a systematic review and meta-analysis
Authors: Yang Yanxu , Ciarletta Matt , Pan Yunqi , Chinchilli Vernon M. , Ssentongo Paddy , Ssentongo Anna E 
Published: May 2020

Abstract
Aim To estimate the pooled prevalence and incidence of depression and its common comorbid mental disorders in persons with a malarial infection and its neurological complications Method We will conduct a systematic review and meta-analysis of studies published between January 1, 1960 and January 1, 2020, reporting the prevalence or incidence of common mental disorders and the risks in people with malaria. We will search the following databases: PubMed (MEDLINE), Scopus, OVID (HEALTH STAR), OVID (MEDLINE) and Joana Briggs Institute EBF Database. No age, geographical location, study-design or language limits will be applied. If multiple languages were used to describe and publish the same data, the English version was selected. This protocol was developed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols guidelines. Three reviewers (MC, YP, YY) will independently screen citations, abstracts and will identify full-text articles for inclusion, extract data and appraise the quality and bias of included studies. Discrepancies will be resolved by consensus or consultation with a fourth researcher (AS). Risk of bias of included studies will be assessed by the Newcastle-Ottawa Quality Assessment Scale. The primary outcomes will be the overall prevalence or incidence of depression and common comorbid mental disorders (CCMDs) in persons with malaria. We will use the random-effects model with a logit transformation of proportions for the pooling of studies. We will assess the between-study heterogeneity using I2 statistics, and Cochrane’s Q statistic (significance level < 0.05). We will perform subgroup meta-analyses to investigate geographical differences in mental disorders and risks of different mental disorders. We will conduct a meta-regression analysis, using study level median age, race and gender proportions, the proportion of study population with malaria, and percentage of the study population with a diagnosis of common mental disorders. We will report absolute differences in the overall probability of common mental disorders. The Egger’s test and funnel plots will be used to assess publication bias.
Evidence of vector borne transmission of Salmonella enterica subsp. enterica ser. Gallinarum and fowl typhoid mediated by the poultry red mite, Dermanyssus gallinae (De Geer, 1778)
Authors: Cocciolo Giulio , Circella Elena , Pugliese Nicola , Lupini Caterina , mescolini Giulia , Catelli Elena , Borchert-Stuhlträger Monika , Zoller Harmut , Thomas Emmanuel , Camarda Antonio 
Published: May 2020

Abstract
Background
The poultry red mite Dermanyssus gallinae (De Geer 1778) is a major ectoparasite of poultry. Infestations are found in most laying hen farms in Europe, and breeder flocks have also been reported to be affected. Mite infestation has detrimental effects on animal welfare, are a cause of significant economic losses, and D. gallinae is often considered as a pathogen vector. Despite suspicion of a close relationship between the poultry red mite and Salmonella enterica subsp. enterica ser. Gallinarum (S. Gallinarum), the causative agent of fowl typhoid (FT), there has been no definitive proof of mite-mediated transmission. Therefore, an investigation was conducted to determine if D. gallinae-mediated transmission of S. Gallinarum could be demonstrated among four different hen groups.
Methods
Two groups of 8 SPF hens (A and B) were experimentally infected with S. Gallinarum in two isolators. After seven days, when birds showed signs of FT, about 25,000 mites were introduced. After 3 days, mites were harvested and used to infest other two SPF hen groups of 8 (C and D), in two separate isolators. Health status of hens was constantly monitored; detection and quantification of S. Gallinarum were performed by PCR and qPCR from mites and organs of dead hens. The maximum likelihood estimation of the infection rate and mite vectorial capacity were calculated.
Results
Clinical disease was observed in groups infected with S. Gallinarum (A and B) and in hens of groups C and D infested with mites harvested from the isolators containing groups A and B. In all 4 groups, S. Gallinarum was detected from liver, spleen, ovary and cecum of hens, thus confirming the diagnosis of FT. Mite analysis demonstrated the presence of the pathogen, with an estimated infection rate ranging between 13.72 and 55.21 infected per thousand mites. Vectorial capacity was estimated to be 73.79.
Conclusion
Mites harvested from birds infected with S. Gallinarum were shown to carry the mite, and then to transfer S. Gallinarum to isolated groups of pathogen-free birds that subsequently showed signs of FT. Mite vectorial capacity was high, demonstrating that D. gallinae should be considered an effective vector of FT.
Morphological characteristics of the Lisfranc ligament
Authors: Yukako Suzuki , Edama Mutsuaki , Fmiya Kaneko , Masahiro Ikezu , Kanta Matuszawa , Ryo Hirabayashi , Ikuo Kagayama 
Published: May 2020

Abstract
Background
This study aimed to clarify the morphological characteristics of the Lisfranc ligament and the plantar ligament.
Methods
Forty legs from 20 cadavers were examined. Classification proceeded according to the number of fiber bundles in the Lisfranc ligament and the plantar ligament. Morphological features measured were fiber bundle length, width, thickness, and angle.
Results
In Type I-a, the Lisfranc and plantar ligaments were a single fiber bundle; in Type I-b, the Lisfranc ligament was a single fiber bundle, and the plantar ligament was two fiber bundles; in Type II-a, the Lisfranc ligament was a two fiber bundle, and the plantar ligament was a single fiber bundle; in Type II-b, the Lisfranc ligament and the plantar ligament were two fiber bundles; in Type III-a, the Lisfranc ligament was three fiber bundles, and the plantar ligament was a single fiber bundle; in Type III-b, the Lisfranc ligament was three fiber bundles, and the plantar ligament was two fiber bundles; in Type IV, the Lisfranc ligament and the plantar ligament could not be separated. Type I-a was seen in 37.5%, Type I-b in 10%, Type II-a in 30%, Type II-b in 7.5%, Type III-a in 7.5%, Type III-b in 2.5%, and Type IV in 5%. The Lisfranc ligament was significantly larger than the plantar ligament in total fiber bundle width, total fiber bundle thickness, and total fiber bundle angle.
Conclusion
The Lisfranc ligament had three fiber bundles, and the plantar ligament had two fiber bundles; classifications were four types and two subgroups.
Effectiveness and safety of extracorporeal shock wave treatment for low back pain:a systematic review and meta-analysis of RCTs
Authors: Ma Jinhui , Yan Yan , Wang Bailiang , Sun Wei , Yue Debo , Wang Weiguo 
Published: May 2020

Abstract
Background

Extracorporeal shock wave therapy (ESWT) has been widely used in musculoskeletal disorders. This meta-analysis was designed to assess the effectiveness and safety of ESWT for patients with low back pain (LBP).

Methods

Multiple electronic databases including Pubmed, Embase, Cochrane’s library, China National Knowledge Infrastructure (CNKI), and Wanfang Data were searched until December, 2019 to identify studies assessing the effectiveness and safety of EPSW for LBP. The prime outcome is pain intensity measured by Visual Analog Scale (VAS) or numeric rating scale (NRS). Other outcomes included functional status, quality of life, psychological outcomes measured by Oswestry Disability Index (ODI), as well as the adverse events. Mean differences were calculated for continuous outcomes, while odd ratios were calculated for binary outcomes. Revman 5.3 software was used for statistical analysis.

Results

Five randomized controlled trials (RCTs) were finally included in this meta-analysis. The pooled mean difference in post-treatment pain scores was −2.37 (P <0.0001), indicating that post-treatment pain scores was significantly higher by 2.37 in control group than in ESWT group. At a mean follow-up time of 4-6 weeks, the pooled mean difference in ODI scores was −14.10 (P <0.00001), indicating that the pooled mean difference of post-treatment ODI scores was 14.10 higher in control group than in ESWT group.

Conclusions

The use of focused ESWT is effective in alleviating pain and improving the general fuctional state for patients with LBP. However, more evidence was needed to verify its safety.