bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adolescent physical activity: role of the level of physical activity and parental social support
Authors: Camargo Edina Maria de , López-Gil Jose Francisco , Piola Thiago Silva , Mota Jorge , Campos Wagner de 
Published: July 2020

Abstract
Background: Maximising the level of physical activity in adolescents has been a concern for many researchers given its multiple benefits for health. To verify the association between adolescents compliance with moderate-to-vigorous physical activity (MVPA) and their parental or legal guardian social support (SS) and MVPA compliance. Methods: Cross-sectional study that selected 1390 (59.6% female) adolescents. The questionnaires IPAQ, QAFA and ASAFA were used. Results: In relation to boys, receiving incentives from parents who “assist always” (OR=1.96 – CI95%=1.16–3.32) and having parents/legal guardians who meet the MVPA recommendations (OR=2.78 – CI95%=1.76–4.38) was associated with meeting the MVPA recommendations. The probability increases when adjusted by socioeconomic status (OR=3.47 – CI95%=1.73–6.96) or education level (OR=4.20 – CI95%=1.96–9.02). Regarding girls, parents who “encourage sometimes” was associated negatively (OR=0.61 – CI95%=0.37–0.98) and meet the MVPA recommendations (OR=2.04 – CI95%=1.46–2.87) was associated positively with meeting the recommendations for adolescents. The likelihood raises when adjusted by socioeconomic status (OR=2.11 – CI95%=1.36–3.29) or education level (OR=4.30 – CI95%=2.41–7.69). Conclusions: Having parents who meet the physical activity recommendations demonstrated higher results in adolescents engaging 60 minutes of MVPA daily when compared to receiving social support, for both boys and girls.
A Randomised Dose-Response Study of Prophylactic Methoxamine Infusion for Preventing Spinal-induced Hypotension during Cesarean Delivery
Authors: Fu Feng , Tang Yu-wen , Chen Hong , Jiao Cui-cui , Ma Na , Chen Xinzhong 
Published: July 2020

Abstract
Background: α-receptor agonists have been reported to be safe and effective for treating or preventing spinal-induced hypotension during cesarean delivery. As a pure α1 adrenergic agonist, methoxamine has potential advantages of reducing myocardial oxygen consumption and protecting the heart in obstetric patients compared to phenylephrine. The aim of this study was to determine the optimal prophylactic methoxamine infusion dose that would be effective for preventing spinal-induced hypotension in 50% (ED50) and 95% (ED95) of parturients. Methods: Eighty parturients with a singleton pregnancy scheduled for elective cesarean delivery were randomly allocated to receive prophylactic methoxamine infusion at one of four different fixed-rates: 1μg/kg/min (group M1), 2μg/kg/min (group M2), 3μg/kg/min (group M3), or 4μg/kg/min (group M4). An adequate response was defined as absence of hypotension (maternal SBP <80% of baseline or SBP < 90 mmHg). The values for ED50 and ED95 of prophylactic methoxamine infusion were determined by probit regression model. The outcomes of maternal hemodynamics and fetal status were compared among the groups. Results: The calculated ED50 and ED95 (95% confidence interval) of prophylactic methoxamine infusion dose were 2.178 (95% CI 1.564 to 2.680) μg/kg/min and 4.821 (95% CI 3.951 to 7.017) μg/kg/min, respectively. The incidence of hypotension decreased with increasing methoxamine infusion dose (15/20, 11/20, 7/20 and 2/20 in group M1, M2, M3 and M4 respectively, P < 0.001). 1-minute Apgar scores and umbilical arterial PaO2 were lower but umbilical arterial PaCO2 was higher in Group M1. No difference was found in the other incidence of adverse effects and neonatal outcomes among groups. Conclusions: Under the conditions of this study, when prophylactic methoxamine infusion was given at a fixed-rate based on body weight for preventing spinal-induced hypotension in obstetric patients, the values for ED50 and ED95 were 2.178μg/kg/min and 4.821μg/kg/min respectively.
Safe driving behaviors in taxi drivers: A predictive cross-sectional study based on the health belief model
Authors: Dadipoor Sakineh , Ranaei Vahid , Ghaffari Mohtasham , Rakhshanderou Sakineh , Safari-Moradabadi Ali 
Published: July 2020

Abstract
Background: The purpose of this study was to predict safe driving behaviors among taxi drivers of Tehran based on the constructs of health belief model. Methods: This descriptive-analytical study was performed on 450 taxi drivers in Tehran using multi-stage sampling. Data were analyzed through SPSS software version 18 using Pearson correlation coefficient and multivariate regression analysis. The P <0.05 was considered statistically significant. Results: Among the constructs of health belief model, perceived severity, perceived benefits and self-efficacy, had significant and direct relationship and perceived barriers had a significant and reverse relationship with safe driving behaviors. According to the results, the constructs of health belief model predicted 17.3% of safe driving behaviors. Self-efficacy was the strongest determinant of safety behaviors (0.362 CI 0.098-0.625). Conclusions: Increasing self-efficacy, reducing perceived barriers and highlighting benefits for the purpose of accepting safe behaviors can be considered as a principle in driving education and training. Also, increasing the perceived severity of adverse outcomes of RTAs and the susceptibility to these outcomes will lead to higher levels of safe driving behaviors.
What has been the progress in addressing financial risk in Uganda? Analysis of catastrophe and impoverishment due to health payments
Authors: Kwesiga Brendan , Aliti Tom , Nabukhonzo Pamela , Najjuko Susan , Byawaka Peter , Hsu Justine , Ataguba John , Kabaniha Grace 
Published: July 2020

Abstract
Background: Monitoring progress towards Universal Health Coverage (UHC) requires an assessment of progress in coverage of health services and protection of households from the impact of direct out-of-pocket payments (i.e. financial risk protection). Although Uganda has expressed aspirations for attaining UHC, out-of-pocket payments remain a major contributor to total health expenditure. This study aims to monitor progress in financial risk protection in Uganda. Methods: This study uses data from the Uganda National Household Surveys for 2005/06, 2009/10, 2012/13 and 2016/17. We measure financial risk protection using catastrophic health care payments and impoverishment indicators. Health care payments are catastrophic if they exceed a set threshold (i.e. 10% and 25%) of the total household consumption expenditure. Health payments are impoverishing if they push the household below the poverty line (the US$1.90/day and Uganda’s national poverty lines). A logistic regression model is used to assess the factors associated with household financial risk.Results: The results show that while progress has been made in reducing financial risk, this progress remains minimal, and there is still a risk of a reversal of this trend. We find that although catastrophic health payments at the 10% threshold decreased from 22.4% in 2005/06 to 13.8% in 2012/13, it increased to 14.2% in 2016/17. The percentage of Ugandans pushed below the national poverty line (US$1.90/day) has decreased from 5.2% in 2005/06 to 2.7% in 2016/17. The distribution of both catastrophic health payments and impoverishment varies across socio-economic status, location and residence. In addition, certain household characteristics (poverty, having a child below 5 years and an adult above 60 years) are more associated with the lack of financial risk protection. Conclusion: There is a need for targeted interventions to reduce OOP payments, especially among those most affected to increase financial risk protection. In the short-term, it is important to ensure that public health services are funded adequately to enable effective coverage with quality health care. In the medium-term, increased reliance on mandatory prepayment will reduce the burden of OOP health spending further.
circRNA-UBAP2 promotes the proliferation and inhibits apoptosis of ovarian cancer though miR-382-5p/PRPF8 axis
Authors: Xu Qin , Deng Bo , Li Manlin , Chen Yang , Zhuan Li 
Published: July 2020

Abstract
Objective: circular RNAs (circRNAs) have been reported to be essential regulators of multiple malignant cancers. However, the functions of circRNAs in ovarian cancer need to be further explored. The aim of our study is to explore the role of circRNA-UBAP2 in ovarian cancer and its mechanism. Results: circRNA-UBAP2 was upregulated in ovarian cancer tissues and cell lines. Knockdown of circRNA-UBAP2 inhibited cell proliferation and promoted cell apoptosis, but circRNA-UBAP2 overexpressed got opposite results. In addition, circRNA-UBAP2 targeted miR-382-5p and downregulated its expression, PRPF8 was a target gene of miR-382-5p. Furthemore, circRNA-UBAP2/miR-382-5p/PRPF8 axis affected the proliferation, apoptosis and cell cycle of ovarian cancer through the mechanism of competing endogenous RNAs (ceRNA). Conclusion: circRNA-UBAP2 acted as a ceRNA to sponged miR-382-5p, increased the expression level of PRPF8, and prompted proliferation and inhibited apoptosis in ovarian cancer cells.
Maternal bile acid profile and subtype analysis of intrahepatic cholestasis of pregnancy
Authors: shao yong , Li Huan , Tang Qing , Xu Di , Chen Siyu 
Published: July 2020

Abstract
Background ICP pregnant women have a unique profile of serum bile acid metabolis, early and accurate identification of ICP patients is beneficial to early appropriate treatment and improvement of pregnancy outcomes. In this study, ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS) was used to analyze the 15 types of serum bile acid profiles of ICP in third trimester, patients with cholelithiasis, and patients with hepatitis B virus. The ICP diagnostic model established by partial least squares-discriminant analysis (PLS-DA) was used to screen the differential bile acids for clinical subtypes of ICP. 144 cases of ICP patients were involved in this study, and were divided into four subgroups according to serum levels of TBA, DBIL, and ALT. Results ①The differential serum bile acid profiles of ICP group and normal pregnant women were DCA, TDCCA, TCA, GDCA and GLCA.②The differential serum bile acid profiles of the ICP1 group (ICP with jaundice) and normal pregnant women were TCDCA, TCA, GCA, GCDCA, TUDCA and GUDCA.③The differential serum bile acid profiles of the ICP3 group (hyperbiliary acidemia of pregnancy) and normal pregnant group was GUDCA, LCA, GLCA, UDCA, TUDCA, CDCA, and TLCA (P <0.05).④The differential serum bile acid profiles of ICP4 group (idiopathic aminotransferase abnormality during pregnancy) and normal pregnant group was UDCA, GUDCA, TUDCA, GCA and GLCA (P <0.05).⑤The occurrence of meconium-stained amniotic fluid, premature delivery and cesarean section in ICP1 group was significantly higher than normal group,ICP2 group,ICP3 group,and ICP4 group (P <0.05); The occurrence of meconium-stained amniotic fluid, premature delivery and cesarean section in ICP2 group, ICP3 group,and ICP4 group was significantly higher than normal group (P <0.05), but no difference was found among ICP2 group, ICP3 group,and ICP4 group (P> 0.05). Conclusion: Maternal serum bile acid profiles are useful to differentiate the four subtypes of ICP. ICP with jaundice could be an important predictor of adverse pregnancy outcomes of ICP.
BrrICE1.1 is associated with putrescine synthesis through regulation of the arginine decarboxylase gene in freezing tolerance of turnip (Brassica rapa var. rapa)
Authors: Yin Xin , Yang Yunqiang , Lv Yanqiu , Li Yan , Yang Danni , Yue Yanling , Yang Yongping 
Published: July 2020

Abstract
Background: In the agricultural areas of Qinghai-Tibet Plateau, temperature varies widely from day to night during the growing season, which makes the extreme temperature become one of the limiting factors of crop yield. Turnip (Brassica rapa var. rapa) is a traditional crop of Tibet grown in the Tibet Plateau, but its molecular and metabolic mechanisms of freezing tolerance are unclear. Results: Here, based on the changes in transcriptional and metabolic levels of Tibetan turnip under freezing treatment, the expression of the arginine decarboxylase gene BrrADC2.2 exhibited an accumulative pattern in accordance with putrescine content. Moreover, we demonstrated that BrrICE1.1 (Inducer of CBF Expression 1) could directly bind to the BrrADC2.2 promoter, activating BrrADC2.2 to promote the accumulation of putrescine, which was verified by RNAi and overexpression analyses for both BrrADC2.2 and BrrICE1.1 using transgenic hair root. The function of putrescine in turnip was further analyzed by exogenous application putrescine and its inhibitor DL-α-(Difluoromethyl) arginine (DFMA) under freezing tolerance. In addition, the BrrICE1.1 was found to be involved in the ICE1-CBF pathway to increase the freezing stress of turnip. Conclusions: BrrICE1.1 could bind the promoter of BrrADC2.2 or CBFs to participate in freezing tolerance of turnip by transcriptomics and targeted metabolomics analyses. This study revealed the regulatory network of the freezing tolerance process in turnip and increase our understanding of the plateau crops response to extreme environments in Tibet.
Psoralen alleviates high glucose-induced HK-2 cell injury by inhibition of Smad 2 signaling via upregulation of microRNA 874
Authors: Lin Yongtao , Zhong Lili , Li Hailun , Xu Yong , Li Xiang , Zheng Donghui 
Published: July 2020

Abstract
Background: Diabetic nephropathy (DN) causes the vast proportion of excess mortality for patients with diabetes. Novel therapeutic approaches slowing down its incidence is still lacking. Psoralen is the major active ingredient of Psoralea corylifolia Linn. (PCL), which was used to treat a number of diseases. In this study, we aimed to investigate whether psoralen could alleviate DN using in vitro model. Methods: Cell viability assay and immunofluorescence were used to evaluate the effect of psoralen on high glucose (HG)-stimulated human kidney HK-2 cells (48 h). RT-qPCR was used to detect the expressions of miRNA in cells. Cell transfection, apoptosis assay, inflammatory cytokines detection and Western blot were further performed to explore the underlying molecular mechanisms.Results: HG-induced toxicity of HK-2 cells was alleviated by psoralen. Meanwhile, the secretion of inflammatory cytokines and extracellular matrix (ECM) accumulation induced by HG in HK-2 cells were also decreased by psoralen. In addition, the expression of miR-874 in HK-2 cells was significantly upregulated by psoralen. Western blot assays indicated that psoralen could reverse HG-induced increase of TLR-4/NF-κB and Smad2 via upregulation of miR-874.Conclusion: This study demonstrated that psoralen could significantly alleviate HG-induced HK-2 cell injury via upregulation of miR-874. In addition, HG-induced increase of TLR-4/NF-κB and Smad2 was revered by psoralen. Therefore, psoralen might serve as an agent for the treatment of DN.
Coronavirus (COVID-19) in Italy: knowledge, management of patients and clinical experience of Italian dentists during the spread of contagion
Authors: Putrino Alessandra , Raso Mario , Magazzino Cosimo , Galluccio Gabriella 
Published: July 2020

Abstract
Background: The coronavirus infection that emerged in China in the last few months of 2019 has now spread globally. Italy registered its first case in the second half of February, and in a short time period, it became the top country in Europe in terms of the number of infected people and the first in the world in terms of deaths. The medical and scientific community has been called upon to manage the emergency and to take measures. Dentists also need to take new precautions during their clinical activity to protect themselves, coworkers and patients from the risks of contagion and to avoid further spread of infection.Methods: Following the data published in the international literature as well as the guidelines and directives constantly updated by the WHO and by the national health authorities, a questionnaire to be completed anonymously was submitted online to Italian dentists using social tools and online professional platforms. The collected data were processed statistically, providing descriptive data and analysis of correlations of the most significant parameters using the Pearson’s χ2, the Likelihood-Ratio χ2, Cramér’s V, Fisher’s exact test, Goodman and Kruskal’s γ, and Kendall’s τb (p<0.05).Results: A total of 535 dentists from Italy participated in the survey. A good level of scientific knowledge about coronavirus and the extra precautionary measures needed to limit the spread was related to the age of respondents and their sex. Coming from areas with higher concentrations of cases affected knowledge, level of attention and perception of risk related to dental activity.Conclusions: At the moment, there are no therapies or vaccines to contain the infection with the new coronavirus that is causing many infections, many of which are fatal, worldwide. Dentists are one of the categories at highest risk of encountering diseases and infections because they work in close proximity with patients, and in their procedures, there is always contact with aerosols with high bacterial and viral potential. Therefore, during this COVID-19 emergency, it is important that dentists are properly informed and take the appropriate precautionary measures.
Detection of Brucella abortus and Brucellla melitensis in cattle and sheep from southern Cameroon
Authors: Mitterran Kamga Ndefo Rolin , Barberine Silatsa Assongo , Oumarou Farikou , Simo Gustave 
Published: July 2020

Abstract
Background: Brucellosis is an infectious disease caused by bacterial of the genus Brucella. Although investigations have identified Brucella antibodies in many animal species, little attention has been paid towards specific identification of different Brucella species in animals in Sub-Saharan African countries. This study was designed to identify Brucella abortus and Brucellla melitensis in cattle and sheep of several villages of southern Cameroon in order to improve our epidemiological knowledge on brucellosis in central AfricaMethods: Blood samples were collected from 597 cattle and 315 sheep from 15 villages of two regions of Cameroon. DNA was extracted from blood samples and primers amplifying the bcsp31 gene locus were used for the identification of Brucella spp infections in these animals. Amongst animals found with Brucella spp infections, specific primes were used to identify B. abortus and B. melitensis respectively. Results: Of the 912 animals analyzed in this study, 159 (17.4%) were infected with Brucella spp. This gives a Brucella infection rate of 20.9% in cattle and 10.8% in sheep. Of the 159 animals harboring Brucella spp infections, 119 (74.8%) were infected by B. abortus and 35 (22.0%) by B. melitensis. The overall infection rates were 18.4% (110/597) for B. abortus and 1.7% for B. melitensis in cattle; 2.9% for B. abortus and 7.9% for B. melitensis in sheep. Co-infections of B. abortus and B. melitensis were found in 9 (1%) animals; 6 (1%) cattle and 3 (1%) sheep. The Brucella infection rate was significantly higher in animals from the Noun division (20.6%) compared to those of Yoko (12.4%). Between animal species, significant differences (P = 0.0001) were observed in infection rates of both abortus and B. melitensis. Within and between sampling sites, significant differences (P = 0.02) were observed in the B. abortus infection rates. Conclusion: This study revealed B. melitensis and B. abortus infections in cattle and sheep from the Noun and Yoko divisions in southern Cameroon. The identification of B. melitensis and B. abortus in animals suggests further investigations on human brucellosis. Results of this study highlight the need of developing and implementing control measures against brucellosis in Cameroon.
Limited Effect of Rehabilitation for Preventing a Decline in Functional Status after Community-Acquired Pneumonia in Elderly Patients
Authors: Chen Hao , Hara Yu , Horita Nobuyuki , Saigusa Yusuke , Hirai Yoshihiro , Kaneko Takeshi 
Published: July 2020

Abstract
Background: Functional status is often decreased after hospitalization in elderly community-acquired pneumonia (CAP) survivors. This study investigated factors contributing to decreased functional status.Methods: This retrospective, observational study was conducted in two medical facilities from January 2016 to December 2018. Hospitalized CAP patients >64 years of age were divided into two groups: a maintained group, without decreased functional status, and a decreased group, with decreased functional status. Functional status was evaluated by the Barthel Index (BI) (range, 0–100, in 5-point increments) and graded into three categories: independent, BI 80–100; semi-dependent, BI 30–75; and dependent, BI 0–25. A decreased functional status was considered as a decline of at least one category. The primary outcome was the length of hospital stay. Results: The maintained group included 400 patients, and the decreased group included 138 patients (median age: 77 vs 82 years; p < 0.001). The decreased group had a longer hospital stay (13 vs 27; p<0.001), with a high rate of rehabilitation [189(47.3%) vs 104(75.4%); p<0.001]. Multivariable regression analysis showed that factors affecting functional status were length of hospital stay, aspiration, age, and pneumonia severity index (PSI) category V (odds ratio 1.05, 95%CI 1.04–1.07; 2.66, 95%CI 1.58–4.49; 1.05, 95%CI 1.02–1.09; and 1.92, 95%CI 1.29-3.44; respectively). Rehabilitation showed a limited effect in preventing a decreased functional status on propensity score analysis (p=0.327).Conclusions: Length of hospital stay, aspiration, age, and PSI V were independent contributors to decreased functional status. Rehabilitation showed a limited effect in preventing decreased functional status.
In situ characterization of calcium fluxes in astrocytic mitochondria from the mouse striatum and hippocampus
Authors: Huntington Taylor , Sriniv Rahul 
Published: July 2020

Abstract
Astrocytes govern critical aspects of brain function via Ca2+ signals, the majority of which associate with mitochondria. However, little is known with regard to in situ sources, kinetics or mechanisms of Ca2+ influx in astrocytic mitochondria. To address this knowledge gap, we expressed the genetically encoded calcium indicator, GCaMP6f within the mitochondrial matrix of adult mouse astrocytes in the dorsolateral striatum (DLS) and hippocampus (HPC). We found spontaneous Ca2+ events in astrocytic mitochondria with subcellular differences between somatic, branch, and branchlet mitochondria, as well as inter-regional differences between astrocytes in the DLS and HPC. We also found a strong dependency of spontaneous mitochondrial Ca2+ fluxes on endoplasmic reticulum stores, the surprising lack of a major role for the mitochondrial calcium uniporter, MCU, and dual mitochondrial Ca2+ responses with multiple neurotransmitter agonists. Together, our findings provide a foundational understanding of mechanisms for Ca2+ influx in astrocytic mitochondria within disease-relevant brain regions.