bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Molecular Biomarkers in Taiwanese Patients with Wilms Tumor Using a Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)-Based Approach
Authors: Lu Meng-Yao , Wang Wen-Chung , Hou Tai-Cheng , Kuo Chen-Yun , Lai Yen-Chein 
Published: September 2020

Abstract
Background: Wilms tumor is a solid tumor that frequently occurs in children. Genetic or epigenetic aberrations in WT1 and WT2 loci are implicated in its etiology. Moreover, tumor suppressor genes are frequently silenced by methylation in this tumor. Methods: In the present study, we analyzed the methylation statuses of promoter regions of 24 different tumor suppressor genes using a methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA)-based approach in six Wilms tumors. Results: All six Wilms tumors showed methylation of RASSF1 specific to tumors, not in normal tissues. Moreover, methylated HIC1 was identified in stromal type Wilms tumors and methylated BRCA1 was identified in epithelial type Wilms tumors. Unmethylated CASP8, RARB, MLH1_167, APC, and CDKN2A were found only in blastemal predominant type Wilms tumors. Conclusions: Our results indicated that methylation of RASSF1 is the essential event in the tumorigenesis of Wilms tumor, which may inform its clinical and therapeutic management. In addition, mixed type Wilms tumors may be classified according to epithelial, stromal, and blastemal components via MS-MLPA-based approach.
Cancer Stem Cell Transcriptome Landscape Reveals Biomarkers Driving Breast Cancer Heterogeneity
Authors: Zhang Zhifa , Chen Xiao , Zhang Jianying , Dai Xiaofeng 
Published: September 2020

Abstract
BackgroundBreast cancers are heterogeneous diseases with distinct clinical outcomes and cancer stem cell percentages. Exploring breast cancer stem cell landscape could help understand the heterogeneity of such cancers with profound clinical relevance.MethodsWe conducted transcriptional profiling of cancer stem cells and non-cancer stem cells isolated from 3 triple negative breast cancer cell lines, analyzed the cancer stem cell transcriptome landscape that drives breast cancer heterogeneity through differential expressed gene analysis, gene ontology and pathway enrichment as well as network construction, and performed experimental validations on the network hub gene.ResultsWe identified a cancer stem cell feature panel consisting of 122 and 381 over-represented and under-expressed genes capable of differentiating breast cancer subtypes. We also underpin the prominent roles of the PI3K-AKT pathway in empowering cancer cells with uncontrolled proliferative and migrative abilities that ultimately foster cancer stemness, and reveal the potential promotive roles of ATP6V1B1 on breast tumor stemness through functional in vitro studies. ConclusionsOur study contributes in identifying a cancer stem cell feature panel for breast tumors that drives breast cancer heterogeneity at the transcriptional level, which provides a reservoir for diagnostic marker and/or therapeutic target identification once experimentally validated as demonstrated by ATP6V1B1.
Epidemiological and Clinical Characteristics and Risk Factors for Mortality of Inpatients with COVID-19 in Golestan Province, Iran: A Retrospective Cohort Study
Authors: Honarvar Mohammad Reza , Roshandel Gholamreza , Shirzad-Aski Hesamaddin , Tabarraei Alijan , Tahamtan Alireza , Ghelichi-Ghojogh Mousa , Fazel Abdolreza , Arefnia Serajeddin , Jafari Nahid , Mansoury Mohsen , Rajabi Abdolhalim 
Published: September 2020

Abstract
Background: Coronavirus disease 2019 (COVID-19) is an emerging infectious disease that was first reported in Wuhan, China, and has subsequently spread worldwide. We aimed to further clarify the epidemiological and clinical characteristics of 2019-nCoV pneumonia and risk factors associated for mortality. Methods: In this retrospective cohort study, we included inpatient with acute respiratory distress syndrome at Golestan University of Medical Sciences Hospitals (Golestan province, Iran) who had been discharged or had died in 2020. Epidemiological, clinical, and laboratory data, including samples for viral RNA detection, were extracted from electronic medical records and compared between recovered and died cases. We used multiple logistic regression methods to explore the risk factors associated with in-hospital death.Results: In overall 2,835 acute respiratory distress syndrome patients were included in this study, of these patients, 874 (30.83.9%) were positive for 2019­nCoV, and 1,046 (36.90%) were negative and 915 (32.28%) were not available for PCR result. Five hundred and sixty-three patients (19.86%) died, 1,687 patients (59.51%) were recovered, and 585 (20.63%) under treatment. Of the total deaths, only 288 (10.15%) were attributed to COVID-19. The most common symptoms at onset of illness were respiratory distress (1,795 [63.32%]), fever (1,601 [56.47%]), dry cough (1,595 [56.26%]), Sore throat (445 [15.70%]), and myalgia (342 [12.06%]).One thousand and twelve (35.7%) had 1 or more coexisting medical conditions. In multiple logistic regression analysis, risk factors associated with the death included older age (OR (Odds Ratio), 1.03; 95% CI; 1.02-1.04), blood oxygen level (SpO2<93%) (OR, 2.44; 95% CI; 1.79-3.31), comorbidities (OR, 2.15; 95% CI; 1.62-2.84), respiratory distress (OR, 1.74; 95% CI; 1.28-2.37), and headache (OR, 0.44 95% CI; 0.21-0.92). Conclusions: The 2019-nCoV infection caused collections of severe respiratory illness and was associated high ratio of hospitalization in ICU and high mortality. Older age and comorbidities were associated with more risk of death among patients with 2019­nCoV.
WITHDRAWN: Detection of Political Intent Through Analysis of Tweets and Homophily Elements
Authors: SHETTY NISHA P , Muniyal Balachandra , Teja Daita Ravi , Maben Leander 
Published: September 2020

Abstract
Twitter is one of the most popular social networking platforms of today’s generation and is a fundamental tool in harvesting the data of many users worldwide. Many discussions ranging from current affairs, news sharing, filing complaints to advertising and discussing some common interests etc. happen on Twitter. It is widely used by many famous politicians as a prominent communication medium to address large masses ,owing to its mass usage and popularity . Thus, it is safe to assume that people communicate their political ideologies on Twitter. Many works have been done so far to deduce the stance of user towards a particular party by performing sentiment analysis on their tweets using popular classifiers. To find connected and similar users, earlier works generated a social network graph, based on the assumption that friends and followers share similar interests, which might not be true in all cases. In contrast, the proposed work employs the concept of ensemble classifier (a single classifier generated from several base learning classifiers) to analyze the tweets and makes use of multiple interaction elements like followers/ following, mentions, re-tweets ,hash tags etc. to infer which political party a user identifies with. These interaction elements project out the homophily (users who share same beliefs and choices) amongst the users. The proposed study can be encompassed to any domain and can be used by advertising agencies, marketing companies, e-commerce, heath care etc. to identify their target audience.
A Randomized, Double Blind, Vehicle Controlled, Parallel, Phase Ii Study to Evaluate Efficacy and Safety of Bac in Patients With Alzheimer’s Disease or Vascular Dementia
Authors: Thein Stephen , Konis George , Lacey Dennis Michael , Hudefi Fayz , Vandersluis Joel , Papka Michelle , Sharma Sanjiv , Anderson Corey G. , Jagadeesan Singaravelu , Fink Evgeny , Pai Ming-Chyi 
Published: September 2020

Abstract
Background: Dementia causes a long-term and gradual cognitive decline and there is no treatment to slow or stop the progression of dementia. Methods: Middle cerebral artery occlusion in rats was employed to investigate the effects of BAC in neural inflammation. A phase II study was conducted in Alzheimer’s disease or vascular dementia patients. BAC or matched vehicle was applied topically on the subject’s external nasal skin, scalp, and neck, twice daily, 30 grams per day for 12 weeks. Cognitive evaluation questionnaires including ADAS-Cog, ADCS-ADL, MMSE, NPI, CIBIS, and CIBIC-plus scores were performed to evaluate the efficacy. A responder analysis was conducted with stratification of different sub-groups of receiving/not receiving dementia medication or donepezil hydrochloride and type of dementia.Results: The responder analysis showed that the naïve and not using donepezil hydrochloride sub-groups in the BAC-treated group had a higher responder rate compared to using donepezil hydrochloride and any dementia medication sub-groups. The BAC group showed a higher percentage of responders than the vehicle group in the mixed dementia population.Conclusion: BAC was safe for human use. BAC improved the responder rate in naïve dementia patients regardless of dementia type and in patients not receiving donepezil hydrochloride. The bigger sample size is required to confirm the efficacy of BAC.Trial registration: NCT02886494 (First posted: September 1, 2016)
Nanosized octahedral LiNi0.5Mn1.5O4 with predominant (111) facet as high performance cathode for Lithium-ion batteries
Authors: Rong Haibo , Deng Binglu , Chen Dongchu , Chen Min 
Published: September 2020

Abstract
Nanosized octahedral LiNi0.5Mn1.5O4 with predominant (111) facet has been successfully fabricated using Mn3O4 nanoparticles precursors via a two-step synthesis, which involves a hydrothermal treatment and the subsequent calcination. The physical properties of the Mn3O4 precursor and the resultant LiNi0.5Mn1.5O4 were characterized by XRD (X-ray diffraction), TEM (transmission electron microscopy) and SEM (scanning electron microscopy). The charge-discharge tests show that the resultant LiNi0.5Mn1.5O4 exhibits excellent cyclability and rate capability, which delivers a discharge capacity of about 117 mAh g− 1 after 300 cycles, and maintains 94% of its initial discharge capacity (124.7 mAh g− 1) at 1C, even at a rate of 40C, a specific capacity of 99.2 mAh g− 1 could be still obtained for the O-LNMO. The superior electrochemical performance of the LNMO is mainly attributed to the synergistic effect of the nanosized octahedral structure and exposed (111) facets of the prepared LiNi0.5Mn1.5O4. We found that the nanosized octahedral structure can not only accommodate the lattice stress caused by John-Teller distortion but also provide short paths for Li+ ion transportation in the material. Additionally, the obtained predominant (111) facet is helpful to the formation of protective SEI film on the spinel LiNi0.5Mn1.5O4 electrode.
Preparation and properties of B4C-TiB2 ceramics prepared by spark plasma sintering
Authors: Fan Jingzhe , Shen Weixia , Zhang zhuangfei , Fang Chao , Zhang Yuewen , Chen Liangchao , Wang Qianqian , Wan Biao , Jia Xiaopeng 
Published: September 2020

Abstract
By doping titanium hydride (TiH2) into boron carbide (B4C), a series of B4C + x wt% TiH2 (x = 0, 5, 10, 15 and 20) composite ceramics were obtained through spark plasma sintering (SPS). The effects of sintering temperature and the amount of TiH2 additive on the microstructure, mechanical and electrical properties of the sintered B4C-TiB2 composite ceramics were investigated. Powder mixtures of B4C with 0−20 wt% TiH2 were heated from 1400 to 1800 °C for 20 minutes under 50 MPa. The results indicated that higher sintering temperatures contributed to greater ceramic density. With increasing TiH2 content, titanium diboride (TiB2) formed between the TiH2 and B4C matrix. This effectively improved Young’s modulus and fracture toughness of the composite ceramics, significantly improving their electrical properties: the electrical conductivity reached 114.9 S·cm−1 at 1800 °C when x = 20. Optimum mechanical properties were obtained for the B4C ceramics sintered with 20 wt% TiH2, which had a relative density of 99.9 ± 0.1%, Vickers hardness of 31.8 GPa and fracture toughness of 8.5 MPa·m1/2. The results indicated that the doping of fine Ti particles into the B4C matrix increased the conductivity and the fracture toughness of B4C.
The effect of heteroatoms on the formation of magnetic ceramic nanocomposites in pyrolysis of organometallic precursors: similar molecular structure, but totally different morphology, composition and properties
Authors: Ruan Zhijun , Ran Jingwen , Liu Shanshan , Chen Yanmei , Wang Xichao , Shi Jie , Zhu Lihong , Zhao Shengfang , Lin Junqi 
Published: September 2020

Abstract
Four organometallic compounds were synthesized for solid-state pyrolysis (SSP) to research the structure-property relationship between the precursors and the as-generated magnetic ceramic nanocomposites (MCNs). In which, the only saturated carbon atom in MC was replaced by N or O or S atom, to produce MN, MO and MS, respectively. It was found that, the crystal phase of the cobalt catalyst could be regulated by introducing different heteroatoms during the pyrolysis: fcc-Co for MC/MN, while fcc/hcp-Co hybrid for MO/MS. Metal cobalt with different crystal phases has their special catalytic and magnetic properties. Thus, MCNs with totally different morphology, composition and properties could be prepared by just changing one heteroatom in the precursors upon SSP. Uniform nanotubes were generated from pyrolysis of MC/MN, while nanospheres were generated from MO/MS. The obtained MCNs all show excellent magnetic properties with Ms ranged from 47.6 to 54.2 emu g-1. Analyzing carefully, due to the magnetic difference between fcc-Co and hcp-Co, the Ms of the MCNs obtained from MO/MS were slightly lower than those of MC/MN, but, their Mr and Hc were 2 to 5 times higher than the latter.
Regulatory mechanism of lncRNA HCP5 in nasopharyngeal carcinoma cell proliferation and apoptosis via the miR-140-5p/NPR3 axis
Authors: Huang Bo , Li Yiliang , Deng Zhuoxia , Lan Guiping , Si Yongfeng , Zhou Qiao 
Published: September 2020

Abstract
Objectives

Nasopharyngeal carcinoma (NPC) is malignant tumor frequently occurring in east and southeast Asia. This study investigated the underlying mechanism of lncRNA HCP5 in NPC cell proliferation and apoptosis.
Methods

LncRNA HCP5 expression in NPC cells and tissues was detected. SUNE-1 cells were transfected with HCP5 silencing and CNE-1 cells were transfected with overexpressing HCP5, and then cell proliferation and apoptosis were measured. Subcellular localization of HCP5 was analyzed. The binding relationship between lncRNA HCP5 and miR-140-5p, and miR-140-5p and NPR3 were predicted and verified. Expressions of miR-140-5p and NPR3 in NPC cells were detected. SUNE-1 cells and CNE-1 cells were transfected with miR-140-5p mimic, and si-HCP5-transfected SUNE-1 cells were treated with miR-140-5p inhibitor. Then the proliferation and apoptosis of NPC cells were detected.
Results

LncRNA HCP5 was elevated in NPC tissues and cells. HCP5 silencing suppressed NPC cell proliferation and enhanced apoptosis. HCP5 was mainly located in the cytoplasm of NPC cells. HCP5 promoted NPR3 expression by competitively binding to miR-140-5p. Overexpression of miR-140-5p weakened proliferation and facilitated apoptosis of NPC cells. miR-140-5p inhibitor partially restored the effects of HCP5 silencing on NPC cells.
Conclusion

LncRNA HCP5 upregulated NPR3 expression via sponging miR-140-5p, thereby suppressing NPC cell proliferation and promoting apoptosis. This study may offer new insights into NPC treatment.
Clinical value and characteristic of absolute counts of lymphocyte subsets in patients with pulmonary tuberculosis: a large-scale Hospital-Based Study
Authors: Liang Jianqin , Bai Xuejuan , An Huiru , Wang Tao , Wang Zhongyuan , Liu Yinping , Xue Yong , Wu Xueqiong 
Published: September 2020

Abstract
Background

This research aimed to investigate the clinical value and characteristics of peripheral blood lymphocyte subsets in tuberculosis (TB) patients by flow cytometry.
Methods

The Absolute counts of T, CD4+ T, CD8+ T, NK, NKT and B lymphocytes were detected in 217 cases of pulmonary TB (PTB), 163 cases of PTB & extra-PTB and 65 cases of extra-PTB patients. We analyzed the change characteristic of subset counts with the clinical parameters in PTB and compared the subsets differences among PTB, PTB with extra-PTB, and extra-PTB patients.
Results

The absolute counts of six subsets in 75.3% of PTB patients were lower than the normal reference range, and 44% patients showed lower CD4+ lymphocytes. (1) The absolute counts of T, CD4+ T, CD8+ T and B lymphocytes was significantly lower in patients older than 60 years old. (2) The NKT cell counts was significantly lower in female patients than the males; (3) 40.8% of patients with positive etiological results and 49.2% of extra-PTB patients showed lower CD8+ T and NK cell counts below the reference range respectively. (4) The T and CD4+ T lymphocyte counts in PTB with positive IGRA were significantly higher than those with negative IGRA; (5) The T and CD8+ T cell counts in PTB with positive IgG antibody were decreased; (6) The T, CD4+ T, CD8+ T and B cell counts reduced with the increasing lesion lobe numbers, accelerated ESR, complications with anemia and low serum albumin, and NK cells also decreased in patients with anemia and low serum albumin; and the T, CD8+, and B cells significantly decreased in PTB patients with diabetes, while NKT increased.
Conclusions

The immune function in most TB patients is impaired and the absolute counts of lymphocyte subsets could be used as the evidence for immune intervention and monitoring the curative effect.
SPOCK1 and POSTN are Valuable Prognostic Biomarkers and Correlate With Tumor Immune Infiltrates in Colorectal Cancer
Authors: Li Mengting , Li Wenjie , Wang Haizhou , Peng Yanan , Hu Qian , Wei Wanhui , Gan Caiqin , Wang Fan , Liu Lan , Zhao Qiu 
Published: September 2020

Abstract
BackgroundImmune cells and stromal cells in the tumor microenvironment (TME) play a vital role in the initiation and progression of colorectal cancer (CRC). The study aimed to screen valuable prognostic biomarkers in CRC on the basis of stromal and immune scores.MethodsWe used the ESTIMATE algorithm to calculate the immune and stromal scores of CRC samples in TCGA. Then the CRC samples were divided into high and low score groups based on the median value of the immune and stromal scores. Differentially expressed genes (DEGs) associated with immune score and stromal score were screened. WGCNA and univariate COX regression analysis were performed to further identify key prognostic genes. The prognostic value of key genes was validated based on The Gene Expression Profiling Interactive Analysis (GEPIA) and GSE17536 dataset. TIMER and CIBERSORT algorithms were applied to analyze the correlations among key genes and tumor-infiltrating immune cells. Results1314 upregulated and 4 downregulated genes associated with immune score and stromal score were identified, which were significantly enriched in immune-related biological processes and pathways. Among these DEGs, SPOCK1 and POSTN were identified as key prognostic genes. High expression of SPCOK1 and POSTN was associated with advanced clinical stage, T stage, N stage, and poor prognosis of CRC. The results from CIBERSORT and TIMER revealed that SPOCK1 and POSTN were associated with tumor-infiltrating immune cells, especially macrophages and neutrophils. Besides, SPOCK1 and POSTN expression were positively correlated with the expression of immune checkpoints.ConclusionCollectively, our results indicate that SPOCK1 and POSTN may be novel prognostic biomarkers in CRC and correlate with immune infiltrates.
Genetic Features of P. falciparum Parasites Collected in 2012-2016 and Anti-Malaria Resistance Along China-Myanmar Border
Authors: Li Mei , Liu Hui , Tang Linhua , Yang Henglin , Bustos Maria Dorina Geluz , Tu Hong , Ringwald Pascal 
Published: September 2020

Abstract
Backgrounds The therapeutic efficacy studies (TES) of T(DHA-PIP) for uncomplicated P. falciparum patients were implemented during 2012-2016 along China (Yunnan province)-Myanmar border, which verified the high efficacy of DHA-PIP. The study focusing on the genetic features of falciparum parasites and basing on in vivo parasite clearance time (PCT) were carried out to explore if they had produced potential resistance to DHA and PIP at molecular level. Methods The genetic features were investigated based on K13 propeller genotypes, Copy numbers of genes pfPM2 and pfmdr1 and 9 microsatellite loci (Short Tandem Repeats, STR) flanking the K13 gene on chromosome 13. The PCT 50s basing on different K13 genotypes, sites, periods and copy numbers were compared.Results In NW (North-West Yunnan province bordering with Myanmar) area, F446I prevalence was 58.96% (79/134). No significant different PCT 50s presented among 3 K13 groups (Chi-Square=2.35, P=0.31, df=2) classified by K13 genotypes within NW area, but all of them were significantly shorter than that in SW (South-West Yunnan province bordering with Myanmar) area (P=0.036, t=-2.11, df=174) where isolates only showed wild K13 genotype. For the copy numbers of pfmdr1 gene, 14.63% (18/123) and 62.5% (10/16) parasite isolates showed double copies (≥ 1.6) in NW and SW areas separately, but for those of pfM2 gene, no parasite isolates did. Between isolates with single and double copies of pfmdr1 gene, no different PCT 50 presented. According to the mean He values, the four K13 groups were arranged as ML group (Menglian County in SW), F446I group, Others (Non-F446I K13 mutation) group and W (wild K13 genotype) group from low to high. The mean Fst values between ML and W groups were significantly higher than other 2 K13 group-W pairs (Paired-T Test: t=-2.659，df=8，P=0.029; t=-4.966, df=8，P=0.001). Conclusions According to our study, P. falciparum isolates in NW area and SW area are very different in genetic features. Artemisinin partial resistance, inferred from genetic marker, F446I, had independently appeared and spread in NW area during 2012-2016. With global application of ACTs, this pattern of artemisinin resistance producing was worth more attention. Fortunately, PIP-resistant feature basing on genetic analysis showed negative results in both areas. So, DHA-PIP was still recommended in antimalarial treatment along China-Myanmar border based on molecular data, which was agreed with the conclusion drawn from in vivo data obtained with same falciparum isolates. Trial registration: ISRCTN, ISRCTN 11775446. Registered 13 April 2020 - Retrospectively registered, http://www.isrctn.com/ISRCTN11775446.
High Efficiency Refrigeration and Pyroelectric Energy Harvesting Applications of Lead Titanate Based Relaxer Ceramic
Authors: Zhang Tianfu , Huang Xianxiong , Xu Hu , Sun Shujie , Liu Zuodong , Tang Xin-Gui 
Published: September 2020

Abstract
Relaxer ferroelectrics are highly attractive in electrocaloric due to large breakdown electric field and wide phase transition temperatures, which has great potential applications in solid-state cooling. In this work, relaxer ferroelectrics are synthesized by a traditional solid state reaction method. Phase transition and relaxer characteristics are studied by temperature dependent dielectric spectrums. Temperature dependent ferroelectric properties are also studied to calculate electrocaloric effect. Maximum value of refrigeration efficiency η ( ΔT/ΔE ) is about 0.079 K·cm/kV, our result lays a foundation and provides a reference for studying high efficiency solid-state electrocaloric refrigeration. Pyroelectric energy harvesting are studied, maximum pyroelectric energy harvesting is 279 kJ/cm 3 .