bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of Ikbkap/Elp1 in mouse oocytes causes spindle disorganization, developmental defects in preimplantation embryos and impaired female fertility
Authors: Yang Kuo-Tai , Inoue Azusa , Lee Yi-Jing , Jiang Chung-Lin , Lin Fu-Jung 
Published: December 2019

AbstractElongator complexes are well known to be involved in a wide variety of cellular processes; however, their functions in mammalian oocytes have not been characterized. Here, we demonstrated in mice that specific deletion of one of the core subunits,Ikbkap/Elp1, in oocytes resulted in spindle defects and chromosome disorganization without affecting folliculogenesis. In accordance with these findings, we observed thatIkbkapmutant female mice are subfertile. Further analyses uncovered that kinetochore–microtubule attachments are severely compromised inIkbkap-deficient oocytes. Moreover, we revealed thatIkbkapmodulates the acetylation status of α-tubulin in oocytes, which may at least in part mediate the meiotic phenotypes described above by affecting microtubule dynamics and kinetochore function. Finally, we showed that embryos derived fromIkbkap-deficient oocytes exhibit an increased frequency of aneuploidy, digyny, progressive delays in preimplantation development, and severe degeneration before reaching the blastocyst stage. In summary, we identifyIkbkapas an important player in regulating oocyte meiosis by modulating tubulin acetylation for chromosome/spindle organization.
Chromosomal dynamics in space and time: evolutionary history of Mycetophylax ants across past climatic changes in the Brazilian Atlantic coast
Authors: Micolino Ricardo , Cristiano Maykon Passos , Travenzoli Natália Martins , Lopes Denilce Meneses , Cardoso Danon Clemes 
Published: December 2019

AbstractFungus-farming ants of the genusMycetophylaxexhibit intra and interspecific chromosome variability, which makes them suitable for testing hypotheses about possible chromosomal rearrangements that endure lineage diversification. We combined cytogenetic and molecular data fromMycetophylaxpopulations from coastal environments to trace the evolutionary history of the clade in light of chromosomal changes under a historical and geographic context. Our cytogenetic analyses revealed chromosomal differences within and among species.M.morschiexhibited three distinct karyotypes and considerable variability in the localization of 45S rDNA clusters. The molecular phylogeny was congruent with our cytogenetic findings. Biogeographical and divergence time dating analyses estimated that the most recent common ancestor ofMycetophylaxwould have originated at about 30 Ma in an area including the Amazon and Southern Grasslands, and several dispersion and vicariance events may have occurred before the colonization of the Brazilian Atlantic coast. Diversification of the psammophilousMycetophylaxfirst took place in the Middle Miocene (ca. 18–10 Ma) in the South Atlantic coast, while “M.morschi” lineages diversified during the Pliocene-Pleistocene transition (ca. 3–2 Ma) through founder-event dispersal for the Northern coastal regions. PsammophilousMycetophylaxdiversification fits into the major global climatic events that have had a direct impact on the changes in sea level as well as deep ecological impact throughout South America. We assume therefore that putative chromosomal rearrangements correlated with increased ecological stress during the past climatic transitions could have intensified and/or accompanied the divergence of the psammophilousMycetophylax. We further reiterate that “M.morschi” comprises a complex of at least three well-defined lineages, and we emphasize the role of this integrative approach for the identification and delimitation of evolutionary lineages.
From prodigious volcanic degassing to caldera subsidence and quiescence at Ambrym (Vanuatu): the influence of regional tectonics
Authors: Shreve Tara , Grandin Raphaël , Boichu Marie , Garaebiti Esline , Moussallam Yves , Ballu Valérie , Delgado Francisco , Leclerc Frédérique , Vallée Martin , Henriot Nicolas , Cevuard Sandrine , Tari Dan , Lebellegard Pierre , Pelletier Bernard 
Published: December 2019

AbstractEruptive activity shapes volcanic edifices. The formation of broad caldera depressions is often associated with major collapse events, emplacing conspicuous pyroclastic deposits. However, caldera subsidence may also proceed silently by magma withdrawal at depth, more difficult to detect. Ambrym, a basaltic volcanic island, hosts a 12-km wide caldera and several intensely-degassing lava lakes confined to intra-caldera cones. Using satellite remote sensing of deformation, gas emissions and thermal anomalies, combined with seismicity and ground observations, we show that in December 2018 an intra-caldera eruption at Ambrym preceded normal faulting with >2 m of associated uplift along the eastern rift zone and 2.5 m of caldera-wide subsidence. Deformation was caused by lateral migration of >0.4 cubic kilometers of magma into the rift zone, extinguishing the lava lakes, and feeding a submarine eruption in the rift edge. Recurring rifting episodes, favored by stress induced by the D’Entrecasteaux Ridge collision against the New Hebrides arc, lead to progressive subsidence of Ambrym’s caldera and concurrent draining of the lava lakes. Although counterintuitive, convergent margin systems can induce rift zone volcanism and subsequent caldera subsidence.
Fe(III) doped carbon nanodots with intense green photoluminescence and dispersion medium dependent emission
Authors: Stan Corneliu Sergiu , Coroabă Adina , Ursu Elena Laura , Secula Marius Sebastian , Simionescu Bogdan C. 
Published: December 2019

AbstractThe preparation and investigation of Fe(III) doped carbon nanodots (CNDs) with intense green photoluminescence and emission dependence on the dispersion medium are reported. Their unusual photoluminescence is especially highlighted in water where the initial blue emission is gradually shifted to intense deep green, while in other common solvents (chloroform, acetone etc.) this behavior has not been observed. Through embedding in a polymer matrix (e.g., PVA) the color transition becomes reversible and dependent on water content, ranging from a full blue emission, when completely dried, to an intense green emission, when wetted. The preparation path of the Fe(III) doped CNDs undergoes two main stages involving the initial obtaining of Fe(III)–N–Hydroxyphthalimide complex and then a thermal processing through controlled pyrolysis. Morphostructural investigations of the prepared Fe(III) doped CNDs were performed through TG, FT-IR, XPS, DLS, TEM and AFM techniques whereas absolute PLQY, steady state and lifetime fluorescence were used to highlight their luminescence properties. The results issued from structural and fluorescence investigations bring new insights on the particular mechanisms involved in CNDs photoluminescence, a topic still open to debate.
Characterization of A Bifunctional Synthetic RNA Aptamer and A Truncated Form for Ability to Inhibit Growth of Non-Small Cell Lung Cancer
Authors: Wang Hanlu , Qin Meng , Liu Rihe , Ding Xinxin , Chen Irvin S. Y. , Jiang Yongping 
Published: December 2019

AbstractAn in vitro-transcribed RNA aptamer (trans-RA16) that targets non-small cell lung cancer (NSCLC) was previously identified through in vivo SELEX. Trans-RA16 can specifically target and inhibit human NCI-H460 cells in vitro and xenograft tumors in vivo. Here, in a follow-up study, we obtained a chemically-synthesized version of this RNA aptamer (syn-RA16) and a truncated form, and compared them to trans-RA16 for abilities to target and inhibit NCI-H460 cells. The syn-RA16, preferred for drug development, was by design to differ from trans-RA16 in the extents of RNA modifications by biotin, which may affect RA16’s anti-tumor effects. We observed aptamer binding to NCI-H460 cells with KD values of 24.75 ± 2.28 nM and 12.14 ± 1.46 nM for syn-RA16 and trans-RA16, respectively. Similar to trans-RA16, syn-RA16 was capable of inhibiting NCI-H460 cell viability in a dose-dependent manner. IC50 values were 118.4 nM (n = 4) for syn-RA16 and 105.7 nM (n = 4) for trans-RA16. Further studies using syn-RA16 demonstrated its internalization into NCI-H460 cells and inhibition of NCI-H460 cell growth. Moreover, in vivo imaging demonstrated the gradual accumulation of both syn-RA16 and trans-RA16 at the grafted tumor site, and qRT-PCR showed high retention of syn-RA16 in tumor tissues. In addition, a truncated fragment of trans-RA16 (S3) was identified, which exhibited binding affinity for NCI-H460 cells with a KD value of 63.20 ± 0.91 nM and inhibited NCI-H460 cell growth by 39.32 ± 3.25% at 150 nM. These features of the syn-RA16 and S3 aptamers should facilitate the development of a novel diagnostic or treatment approach for NSCLC in clinical settings.
Differential remodeling of the electron transport chain is required to support TLR3 and TLR4 signaling and cytokine production in macrophages
Authors: Ahmed Duale , Roy David , Jaworski Allison , Edwards Alexander , Abizaid Alfonso , Kumar Ashok , Golshani Ashkan , Cassol Edana 
Published: December 2019

AbstractIncreasing evidence suggests that mitochondria play a critical role in driving innate immune responses against bacteria and viruses. However, it is unclear if differential reprogramming of mitochondrial function contributes to the fine tuning of pathogen specific immune responses. Here, we found that TLR3 and TLR4 engagement on murine bone marrow derived macrophages was associated with differential remodeling of electron transport chain complex expression. This remodeling was associated with differential accumulation of mitochondrial and cytosolic ROS, which were required to support ligand specific inflammatory and antiviral cytokine production. We also found that the magnitude of TLR3, but not TLR4, responses were modulated by glucose availability. Under conditions of low glucose, TLR3 engagement was associated with increased ETC complex III expression, increased mitochondrial and cytosolic ROS and increased inflammatory and antiviral cytokine production. This amplification was selectively reversed by targeting superoxide production from the outer Q-binding site of the ETC complex III. These results suggest that ligand specific modulation of the ETC may act as a rheostat that fine tunes innate immune responses via mitochondrial ROS production. Modulation of these processes may represent a novel mechanism to modulate the nature as well as the magnitude of antiviral vs. inflammatory immune responses.
Climate change over the Mediterranean and current destruction of marine ecosystem
Authors: Kim Go-Un , Seo Kyong-Hwan , Chen Deliang 
Published: December 2019

AbstractThe Mediterranean is one of the most vulnerable regions to climate change and its summer climate is known to be affected by the South Asian summer monsoon (SASM) through the monsoon–desert teleconnection. In future, rainfall is expected to increase not only over the SASM area but also over the East Asian summer monsoon (EASM) and equatorial Atlantic regions. Here we show that the remote forcing regions affect the Mediterranean climate in the future. A subset of CMIP5 climate simulations exhibits an increase in the descending motion over the Western Mediterranean in the future. This strengthened subsidence comes from the SASM, EASM, and Atlantic forcings: the SASM and EASM heating induces the Gill-type Rossby wave response, and the Atlantic forcing causes the northeastward wave energy propagation. The sea surface temperature change over the Western Mediterranean is consistent with the subsidence change both in the future and in the recent decades. The chlorophyll-a concentration and fisheries landings have decreased in the recent period along with sea surface temperature warming. Our results suggest that special attention is required to conserve the marine ecosystem in the Mediterranean as climate warms.
Antioxidant, Anticancer Activity and Phytochemical Analysis of Green Algae, Chaetomorpha Collected from the Arabian Gulf
Authors: Haq Samina Hyder , Al-Ruwaished Ghaida , Al-Mutlaq Moudhi Abdullah , Naji Sundus Ali , Al-Mogren Maha , Al-Rashed Sarah , Ain Qura Tul , Al-Amro Abir Abdullah , Al-Mussallam Adnan 
Published: December 2019

AbstractSeaweeds are a group of marine multicellular algae; the presence of antioxidant phytochemical constituents in Seaweed Chaetomorpha sp. extracts has received attention for their role in the prevention of human diseases. This study explores the phytochemical constituents, antioxidant, and anticancer properties of the Cladophoraceae, Chaetomorpha sp. Energy dispersive x-ray spectroscopy (EDX), and Gas chromatography-mass spectrometry (GC/MS) were performed to study the chemical structure and chemical formula. Different concentrations of ethanol and aqueous extracts of Chaetomorpha were used to estimate antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and total flavonoid, phenolic, and tannins content assays. Anti-tumor activity against breast cancer cell lines (MCF-7 and MDA-MB-231) was assessed by 3-(4,5-Dimethylthiazol-2-cyl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. The EDX analysis indicated the presence of oxygen, silicon, and calcium as dominant elements. Antioxidant assays indicated that the ethanol extracts of Chaetomorpha consisted of a total of 189.14 ± 0.99 mg QE/g flavonoid content, 21.92 ± 0.43 mg GAE/g phenolic content and 21.81 ± 0.04 mg GAE/g tannins content. The DPPH radical scavenging assay exhibited higher antioxidant activity IC50 (9.41 ± 0.54 mg/mL) in the ethanol extract. Moreover, it showed high anticancer activity by growth inhibition in the MDA-MB-231 breast cancer cell line and low IC50 (225.18 ± 0.61 µg/mL). GC/MS analysis revealed the presence of Dichloracetic acid (DCA) as the active antitumor constituent of Chaetomorpha sp.; other anticancer compounds identified were Oximes and L-α-Terpinol. The results revealed that the type of Chaetomorpha sp. studied here possesses very unique and novel constituents and active potent antitumor chemical constituents and it can act as a promising antioxidant and anticancer agent for future applications in pharmaceutical industries.
Population reference and healthy standard blood pressure range charts in pregnancy: findings from the Born in Bradford cohort study
Authors: Santorelli Gillian , Lawlor Debbie A. , West Jane , Tuffnell Derek , Farrar Diane 
Published: December 2019

AbstractWomen who develop gestational hypertension are at increased risk of adverse perinatal and longer-term outcomes. Reference charts may aid early detection of raised blood pressure (BP) and in doing so reduce adverse outcome risk. We used repeated BP measurements to produce ‘reference’ (whole population) and ‘standard’ (healthy pregnancies only) gestational-age-specific BP charts for all pregnant women (irrespective of ethnicity) and for White British (WB) and Pakistani (P) women. We included 9218 women recruited to the Born in Bradford study with 74,770 BPs. 19% of the whole population, 11% and 25% of WB and P women respectively were defined as healthy pregnancies. For reference and standard charts, for all women and each ethnic group, SBP/DBP at 12 and 20 weeks gestation was similar before rising at 37 weeks. DBP/SBP of reference charts for all women and for each ethnic group were higher than those of the corresponding standard charts. Compared to WB, P women had lower SBP/DBP at 12, 20 and 37 weeks gestation. To conclude; maternal population BP reference charts are higher compared to standard charts (healthy pregnancies) and are influenced by ethnicity.
Tempo and mode of allopatric divergence in the weakly electric fish Sternopygus dariensis in the Isthmus of Panama
Authors: Aguilar Celestino , Miller Matthew J. , Loaiza Jose R. , González Rigoberto , Krahe Rüdiger , De León Luis F. 
Published: December 2019

AbstractSpatial isolation is one of the main drivers of allopatric speciation, but the extent to which spatially-segregated populations accumulate genetic differences relevant to speciation is not always clear. We used data from ultraconserved elements (UCEs) and whole mitochondrial genomes (i.e., mitogenomes) to explore genetic variation among allopatric populations of the weakly electric fish Sternopygus dariensis across the Isthmus of Panama. We found strong genetic divergence between eastern and western populations of S. dariensis. Over 77% of the UCE loci examined were differentially fixed between populations, and these loci appear to be distributed across the species’ genome. Population divergence occurred within the last 1.1 million years, perhaps due to global glaciation oscillations during the Pleistocene. Our results are consistent with a pattern of genetic differentiation under strict geographic isolation, and suggest the presence of incipient allopatric species within S. dariensis. Genetic divergence in S. dariensis likely occurred in situ, long after the closure of the Isthmus of Panama. Our study highlights the contribution of spatial isolation and vicariance to promoting rapid diversification in Neotropical freshwater fishes. The study of spatially-segregated populations within the Isthmus of Panama could reveal how genetic differences accumulate as allopatric speciation proceeds.
Sample Size Requirements of Glaucoma Clinical Trials When Using Combined Optical Coherence Tomography and Visual Field Endpoints
Authors: Wu Zhichao , Medeiros Felipe A. 
Published: December 2019

AbstractGlaucoma clinical trials using visual field (VF) endpoints currently require large sample sizes because of the slowly-progressive nature of this disease. We sought to examine whether the combined use of VF testing and non-invasive optical coherence tomography (OCT) imaging of the neuroretinal tissue could improve the feasibility of such trials. To examine this, we included 192 eyes of 121 glaucoma participants seen at ≥5 visits over a 2-year period to extract real-world estimates of the rates of change and variability of VF and OCT imaging measurements for computer simulations to obtain sample size estimates. We observed that the combined use of VF and OCT endpoints led to a 31–33% reduction in sample size requirements compared to using VF endpoints alone for various treatment effect sizes. For example, 189 participants would be required per group to detect a 30% treatment effect with 90% power with combined VF and OCT endpoints, whilst 276 and 285 participants would be required when using VF and OCT endpoints alone respectively. The combined use of OCT and VF endpoints thus has the potential to effectively improve the feasibility of future glaucoma clinical trials.
Combined micro X-ray absorption and fluorescence spectroscopy to map phases of complex systems: the case of sphalerite
Authors: Marini Carlo , Rovira Anna Maria Diaz , Ramanan Nitya , Olszewski Wojciech , Joseph Boby , Simonelli Laura 
Published: December 2019

AbstractCombining micro-X-ray absorption spectroscopy (μXAS) and micro-X-ray fluorescence spectroscopy (μXRF) is a promising approach for the investigation of complex multi-phase systems. In this work, we have employed this approach to investigate natural sphalerite, the most common form of Zinc Sulfide. Spatially resolved elemental distribution maps of common 3d metal atoms (Zn, Cu, Ni, Co, and Fe) are superimposed with chemical speciation and structural parameter maps in order to understand the sphaleriteore-formation process and metamorphosis. Chemical speciation and structural parameters have been obtained by analyzing the μXAS spectra collected in several representative points of the sample, after μXRF mapping. In the present case, this X-ray based approach has permitted to determine the spatial distribution of the Zn species in sphalerite. The presence of two main zincite and smithsonite inclusions has been established, with the latter located close to copper impurity center. Since copper is known to remarkably reduce the corrosion resistance of zinc, resulting in the formation of carbonate as the corrosion product, this implies a possible role of Cu in the growth of the carbonate inclusions. The results obtained highlight the efficiency of this method in univocally identifying the spatial distribution of phases in complex systems, thanks to the simultaneous access to complementary information.
A New Method to Reconstruct in 3D the Emission Position of the Prompt Gamma Rays following Proton Beam Irradiation
Authors: Panaino Costanza M. V. , Mackay Ranald I. , Kirkby Karen J. , Taylor Michael J. 
Published: December 2019

AbstractA new technique for range verification in proton beam therapy has been developed. It is based on the detection of the prompt γ rays that are emitted naturally during the delivery of the treatment. A spectrometer comprising 16 LaBr3(Ce) detectors in a symmetrical configuration is employed to record the prompt γ rays emitted along the proton path. An algorithm has been developed that takes as inputs the LaBr3(Ce) detector signals and reconstructs the maximum γ-ray intensity peak position, in full 3 dimensions. For a spectrometer radius of 8 cm, which could accommodate a paediatric head and neck case, the prompt γ-ray origin can be determined from the width of the detected peak with a σ of 4.17 mm for a 180 MeV proton beam impinging a water phantom. For spectrometer radii of 15 and 25 cm to accommodate larger volumes this value increases to 5.65 and 6.36 mm. For a 8 cm radius, with a 5 and 10 mm undershoot, the σ is 4.31 and 5.47 mm. These uncertainties are comparable to the range uncertainties incorporated in treatment planning. This work represents the first step towards a new accurate, real-time, 3D range verification device for spot-scanning proton beam therapy.
Metabolic syndrome agravates cardiovascular, oxidative and inflammatory dysfunction during the acute phase of Trypanosoma cruzi infection in mice
Authors: Lucchetti Bruno Fernando Cruz , Boaretto Natalia , Lopes Fernanda Novi Cortegoso , Malvezi Aparecida Donizette , Lovo-Martins Maria Isabel , Tatakihara Vera Lúcia Hideko , Fattori Victor , Pereira Rito Santo , Verri Waldiceu Aparecido , de Almeida Araujo Eduardo Jose , Pinge-Filho Phileno , Martins-Pinge Marli Cardoso 
Published: December 2019

AbstractWe evaluated the influence of metabolic syndrome (MS) on acuteTrypanosoma cruziinfection. Obese Swiss mice, 70 days of age, were subjected to intraperitoneal infection with 5 × 102trypomastigotes of the Y strain. Cardiovascular, oxidative, inflammatory, and metabolic parameters were evaluated in infected and non-infected mice. We observed higher parasitaemia in the infected obese group (IOG) than in the infected control group (ICG) 13 and 15 days post-infection. All IOG animals died by 19 days post-infection (dpi), whereas 87.5% of the ICG survived to 30 days. Increased plasma nitrite levels in adipose tissue and the aorta were observed in the IOG. Higher INF-γand MCP-1 concentrations and lower IL-10 concentrations were observed in the IOG compared to those in the ICG. Decreased insulin sensitivity was observed in obese animals, which was accentuated after infection. Higher parasitic loads were found in adipose and hepatic tissue, and increases in oxidative stress in cardiac, hepatic, and adipose tissues were characteristics of the IOG group. Thus, MS exacerbates experimental Chagas disease, resulting in greater damage and decreased survival in infected animals, and might be a warning sign that MS can influence other pathologies.