bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sphk1-induced Autophagy in Microglia Promotes Neuronal Injury Following Cerebral Ischemia-Reperfusion
Authors: Lv Manhua , Jiang Yongjia , Zhang Dayong , Yao Dan , Cheng Yuefeng , Zhang Wei , Zeng Yuanyuan 
Published: October 2020

Abstract
Background: Microglial hyperactivation driven by SphK1/S1P signaling and consequent inflammatory mediator production is a key driver of cerebral ischemia-reperfusion injury (CIRI). While SphK1 reportedly controls autophagy and microglial activation, it remains uncertain as to whether it is similarly able to regulate damage mediated by CIRI-activated microglia. Methods: In the present study, we utilized both an in vitro oxygen-glucose deprivation reperfusion (OGDR) model and an in vivo rat model of focal CIRI to test whether Sphk1 and autophagy is expressed in microglia. Western blot analysis was used to estimate the autophagy protein level (LC3 and SQSTM ) at different time points after OGDR. To detect cytokine secretion in microglial supernatants in response to OGDR, we measured the concentration of IL-1β, IL-6 and TNF-α in the culture supernatants using an enzyme-linked immunosorbent assay (ELISA). To evaluate whether microglia subjected to OGDR exhibited neuronal injury, we used a commercially available terminal transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) kit and flow cytometry to detect apoptotic neurons.Results: We determined that in the context of CIRI, microglia upregulated SphK1 and induced autophagy, while inhibiting these changes by lentivirus targeting SphK1 significantly decreased expression of autophagy . Moreover, we determined that autophagic body formation was enhanced in cerebral tissues following I/R. We also explored the impact of SphK1-induced autophagy on microglial inflammatory cytokine production and associated neuronal apoptosis using an in vitro OGDR model system. At a mechanistic level, we found that SphK1 promotes autophagy via the tumor necrosis factor receptor-associated factor 2 (TRAF2) pathway. Conclusion: These results reveal a novel mechanism whereby SphK1-induced autophagy in microglia can contribute to the pathogenesis of CIRI, potentially highlighting novel avenues for future therapeutic intervention in IS patients.
A Qualitative Study on Patient-Centered Care and Perception of Nurses in Primary Healthcare Practice
Authors: Lateef Adepeju , Mhlongo Euphemia Mbali 
Published: October 2020

Abstract
Background: Patient-centered care (PCC) approach has continued to gain recognition globally as the key to providing quality healthcare. However, this concept is not fully integrated into the management of primary health care (PHC) in existing nursing practice due to numerous challenges. Among these challenges is the perception of nursing on PCC in the Primary Health Care system. This study seeks to present the results of qualitative research performed at various selected PHC centers on nurses’ perceptions in PCC practice.Aim: This study aims at exploring the perception of nurses on PCC.Method: A qualitative research approach was adopted for this study. This study involved thirty local government PHC centers located in Osun State of southwest Nigeria. The sample comprised 28 female nurses and 7 male nurses. Data were collected through a semi-structured interview schedule in different sessions that were conducted on a one on one basis. Thereafter, data analysis was performed using thematic analysis and NVivo 12 software to generate themes, subthemes, and codes.Results: The findings of the study revealed a number of perceptions on PCC that were categorized into positive and negative themes. The negative themes include: Poor approach by the nurses and lack of enforcement agency. The positive themes that emerged include: Outcome-driven healthcare, valued care provider, communication to sharpen care, and driven healthcare service. In addition, the findings established a positive correlation between perception and years of working experience. 68.5 % of the participants, who had working experience in the range of 12 – 31 years, expressed a mixed perception of PCC practice. The remaining 31.5 % of the participants interviewed who had working experience in the range of 2, 6 – 11 years, expressed positive perceptions on the provision of PCC. Conclusion: From the findings of this study, there is a need for the government to enforce the utilization and provision of PCC in the PHC setting. This should be done through the provision of an enabling environment in the PHC setting. In addition, the government should provide regulations and monitoring mechanisms in the PHC institutions. Enforcement agencies should also offer opportunities for continuous training to enhance the nursing care skills of nurses to stimulate and sustain improved healthcare services. Lastly, the government should remunerate and promote nurses based on merit as another means of supporting improved healthcare service delivery by the nurses.
MicroRNA-449a Plays an Indicative Role in Diagnosing Lung Cancer
Authors: Yang Jun , Zhong Hua , Yang Qinghui , Yu Jian , Jin Cailing , Li Xiaorui 
Published: October 2020

Abstract
Background: Lung cancer is one of the most common causes of cancer death among all the malignancies worldwide. Evidences suggest that the incidence and mortality of lung cancer has been on the rise. MicroRNA-449a (miR-449a) as one important member of microRNAs, has been demonstrated acting as a tumor suppressor in lung cancer. In this study, we sought to assess the relationship between miR-449a expression level and diagnostic value of lung cancer.Methods: In this present research, quantitative Real-Time PCR was applied to detect the miR-449a expression in 116 lung cancer patients and 41 healthy volunteers. The diagnostic value of miR-449a in lung cancer patients was determined by receiver operating characteristic (ROC) curve.Results: MiR-449a was significantly down-regulated in lung cancer patients compared with healthy control (P<0.05). In addition, miR-449a expression was associated with sex (P=0.004), tumor size (P=0.000), TNM stage (P=0.006) and metastasis (P=0.036). However, there was no correlation with age, smoking history and histological type of lung cancer patients (all P＞0.05). In the ROC analysis, the results showed that the area under the ROC curve (AUC) was 0.902 with the sensitivity of 94.8% and specificity of 78.0%, and the optimum cutoff value was 2.255.Conclusion: MiR-449a expression was down-regulated in lung cancer patients, and it could be an efficient diagnostic biomarker in lung cancer patients.
Autophagy regulation patterns characterization identifies immune phenotypes and immunotherapy response in head and neck squamous cell carcinoma (HNSCC)
Authors: Ma Bo , Li Hui , Zheng Mingzhu , Cao Rui , Yu Riyue 
Published: October 2020

Abstract
BackgroundAutophagy degraded and recycled cytoplasmic components to maintain cellular homeostasis under stress conditions, which was recognized as double-edged sword in oncogenesis and novel target in cancer treatment. However, comprehensive analysis of the relationship between autophagy regulation and immunity has not been reported yet. MethodsUnsupervised consensus clustering algorithm was used to identify autophagy regulation patterns. LASSO cox regression algorithm was used to build a scoring system (ATGscore) to represent the individual autophagy regulation pattern. Then integrated analysis of autophagy regulation patterns and ATGscore was performed.ResultsWe have successful depicted five autophagy regulation patterns and established a scoring system (ATGscore) to represent it, which was shown to be significantly correlated with TIME infiltration, immune phenotypes, molecular subtypes, and genetic variation, etc. in 1165 head and neck squamous cell carcinoma (HNSCC) patients. Moreover, ATGscore was an independent prognostic factor and potent predictor for clinical response to immune-checkpoint inhibitors (ICIs) targeting immunotherapy. ConclusionUnderstanding the molecular characteristics of autophagy regulation patterns in HNSCC could help us to depict the underlying mechanism of tumour immunity and lay a solid foundation on combination of autophagy targeting therapies and immunotherapies for clinical application in HNSCC.
Increased Ratio of sST2/LVMI Predicted Cardiovascular Mortality and Heart Failure Rehospitalization in Heart Failure with Reduced Ejection Fraction Patients: A Prospective Cohort Study
Authors: Li Fuhai , Xu Mengying , Fu Mingqiang , Cui Xiaotong , Zhou Jingmin , Ge Junbo 
Published: October 2020

Abstract
Background: Inflammation is considered to be one of the principal triggering mechanisms for Left ventricular (LV) fibroblast and remodeling in heart failure(HF), which are related to adverse events in HF failure patients. Soluble ST2 (sST2), a member of the interleukin-1 receptor family, is assumed to play a significant role in the inflammatory response of fibroblasts. The present study aimed to investigate the prognostic value of sST2/ left ventricular mass index (LVMI), a parameter of the pre-fibrotic inflammatory phase of heart failure in comparative to remodeling, in the heart failure with reduced ejection fraction (HFrEF).Methods: The present study was a cohort study. A total of 45 consecutive patients with suspected HFrEF from 1/9/2015 to 31/12/2016 were prospectively enrolled. The target-independent variable was the ratio of sST2/LVMI measured at baseline. The primary endpoint was the composite endpoint of cardiovascular-cause mortality or heart failure readmission. The prognostic impact of the ratio of sST2/LVMI was evaluated by multivariable Cox proportional-hazards regression model.Results: 45 patients were enrolled, the average age was 48±14 years old, and about 20% of them were male. Patients were followed for 9 months, during which the primary outcome occurred in 15 patients. By Kaplan–Meier analysis, patients with high ratio of the ratio of sST2/LVMI ≥ 0.39 had shorter event-free survival than the middle ( ratio of sST2/LVMI between 0.39 and 0.24) and low ratio of sST2/LVMI (ratio of sST2/LVMI < 0.24) patients (log-rank, P = 0.022). Results of fully-adjusted multivariable Cox regression analysis showed the ratio of sST2/LVMI was positively associated with the composite outcome of HFrEF patients after adjusting confounders hazard ratio (HR) 1.64, 95% CI (1.06, 2.54). By subgroup analysis, a stronger association was found in patients whose ages between 40 and 55 years old, systolic blood pressure ＜115 or≥129mmHg, diastolic blood pressure< 74 mmHg, hematocrit < 44.5%, and interventricular septum ≥8.5mm.Conclusion: In HFrEF patients, the relationship between the ratio of sST2/LVMI and the composite outcome is linear. A higher baseline ratio of sST2/LVMI levels is associated with increased risk of cardiovascular-cause mortality and HF rehospitalization in patients with HFrEF in the short term follow up.
Current knowledge of Chagas-related heart disease among pediatric cardiologists in the United States
Authors: Malhotra Sanchi , Masood Imran , Giglio Noberto , Pruetz Jay D. , Pannaraj Pia S. 
Published: October 2020

Abstract
BackgroundChagas disease is a pathogenic parasitic infection with approximately 8 million cases worldwide and greater than 300,000 cases in the United States (U.S.). Chagas disease can lead to chronic cardiomyopathy and cardiac complications, with variable cardiac presentations in pediatrics making it difficult to recognize. The purpose of our study is to better understand current knowledge and experience with Chagas related heart disease among pediatric cardiologists in the U.S.MethodsWe prospectively disseminated a 19-question survey to pediatric cardiologists via 3 pediatric cardiology listservs. The survey included questions about demographics, Chagas disease presentation and experience.ResultsOf 139 responses, 119 cardiologists treat pediatric patients in the U.S. and were included. Most providers (87%) had not seen a case of Chagas disease in their practice; however, 72% also had never tested for it. The majority of knowledge-based questions about Chagas disease cardiac presentations were answered incorrectly, and 85% of providers expressed discomfort with recognizing cardiac presentations in children. Most respondents selected that they would not include Chagas disease on their differential diagnosis for presentations such as conduction anomalies, myocarditis and/or apical aneurysms, but would be more likely to include it if found in a Latin American immigrant. Of respondents, 87% agreed that they would be likely to attend a Chagas disease-related lecture.ConclusionsPediatric cardiologists in the U.S. have seen very few cases of Chagas disease, albeit most have not sent testing or included it in their differential diagnosis. Most individuals agreed that education on Chagas disease would be worth-while.
Hybrid Image Steganography Method Using LZW and Genetic Algorithm for Hiding Confidential Data
Authors: Almawgani Abdulkarem , Alhawari Adam , Alarashi Wlaed , Alshwal Ali 
Published: October 2020

Abstract
Digital images are commonly used in steganography due to the popularity of digital image transfer and exchange through the Internet. However, the tradeoff between managing high capacity of secret data and ensuring high security and quality of stego image is a major challenge. In this paper, a hybrid steganography method based on Haar Discrete Wavelet Transform (HDWT), Lempel Ziv Welch (LZW) algorithm, Genetic Algorithm (GA), and the Optimal Pixel Adjustment Process (OPAP) is proposed. The cover image is divided into non-overlapping blocks of nxn pixels. Then, the HDWT is used to increase the robustness of the stego image against attacks. In order to increase the capacity for, and security of, the hidden image, the LZW algorithm is applied on the secret message. After that, the GA is employed to give the encoded and compressed secret message cover image coefficients. The GA is used to find the optimal mapping function for each block in the image. Lastly, the OPAP is applied to reduce the error, i.e., the difference between the cover image blocks and the stego image blocks. This step is a further improvement to the stego image quality. The proposed method was evaluated using four standard images as covers and three types of secret messages. The results demonstrate higher visual quality of the stego image with a large size of embedded secret data than what is generated by already-known techniques. The experimental results show that the information-hiding capacity of the proposed method reached to 50% with high PSNR (52.83 dB). Thus, the herein proposed hybrid image steganography method improves the quality of the stego image over those of the state-of-the-art methods.
Aberrant activated APOBEC3B is associated with p53 mutant-driven refractory/relapsed Diffuse Large B-cell Lymphoma
Authors: Zhang Xuzhao , Wu Zhaoxing , Liang Yun , Li Jinfan , Huang Liansheng , Yu Teng , Xu Yang , Yu Qingfeng , Zhang Xuteng , Li Xiuzhen , Xu Xiaohua , Wang Weiqin , Xu Genbo , Zhang Xiaohong , Lv Qinghua , Fang Yongming , Ma An , Zhao Xiaoying , Yu Xiaofang , Xu Rongzhen 
Published: October 2020

Abstract
BackgroundAlthough treatment of diffuse large B-cell lymphoma (DLBCL) has progressed considerably in recent years, treatment failure still occurs in about 40% of patients who are refractory/relapse. Recent studies suggest that TP53 mutation may be an important cause of refractory/relapse in DLBCL, but the cause of TP53 mutation remains unclear. MethodsIn the present study, the correlation between TP53 mutation status and APOBEC3A and APOBEC3B expression in DLBCL specimens was searched by detecting the correlation between TP53 mutation and APOBEC3B expression in combination with database informatics analysis. Further, the relationship between APOBEC3B expression and TP53 mutation was analyzed by constructing APOBEC3B induced expression DLBCL cell lines. The effects of APOBEC3B-induced TP53 mutants on DLBCL cell proliferation and drug resistance were also tested. ResultsWe identify APOBEC3B as a critical factor that regulates p53-mutant driven drug resistance of DLBCL. APOBEC3B induces TP53 mutations of DLBCL cells, and its mutation patterns are similar to those in DLBCL patients. Moreover, APOBEC3B-induced p53 mutants promoted growth of DLBCL cells as well as contributed to drug resistance. In human DLBCL, APOBEC3B is aberrantly activated and associated with p53 mutant-mediated refractory/relapsed DLBCL. ConclusionThese findings yield insights into the mechanism of refractory/relapsed DLBCL induced by p53 mutants and reveal APOBEC3B as a new therapeutic target.
The effect of dynamic prosthetic alignment on the transtibial gait: Analyzing with Opensim
Authors: Luengas-C Lely , Camargo Esperanza , Garzón Enrique 
Published: October 2020

Abstract
After amputation, fitting of the prosthesis in the adaptation and gait of an individual is crucial. However, this known dynamic alignment process is subjective on the part of the professional who makes said adjustment, compromising the stability of the individual. Therefore, a kinematic model to analyze the effects of dynamic alignment during the transtibial amputees gait in the sagittal plane using Opensim was developed. For this group, the socket position (Flexion and Extension, Abduction and Adduction) and the movements of prosthetic foot (Plantarflexion and Dorsiflexion, Inversion and Eversion) were analyzed. In opensim, the “osim” pilot was amputated incorporating a transtibial prosthesis that was previously modeled in Solid work, with a Matlab script the variation of the position of the prosthesis using inverse kinematics was simulated, results were validated on a subject with wireless sensors for the measurement. The model showed that acceptable gait patterns are found in positive variations between 2 ° and 6° for the socket and 2 ° for the prosthetic foot.
The linear regression equation for pressurised metered-dose inhaler: Using canister weight to predict balance actuation in asthma inhaler
Authors: Gunasekaran Gobi Hariyanayagam , Arif Syazwan Faiz b. Kamal Al , Gunasekaran Shargunan Selvanthan , Gunasekaran Sera Selvanthan Sundram 
Published: October 2020

Abstract
INTRODUCTION Pressurised metered-dose inhalers (pMDIs) are not equipped with dose counters hence the balance actuation in a canister could not be determined. Each actuation expels a considerable amount of active ingredients and excipients from a canister, thus the balance actuation remaining in a pMDI based on canister weight could be evaluated using a linear regression equation.METHODOLOGY New pMDIs of 5 active ingredients [salbutamol (GSK) 200 actuation, budesonide (Glenmark) 300 actuation, ipratropium/fenoterol (Boehringer) 200 actuation, fluticasone (GSK/innovator) 120 actuation, fluticasone (Cipla/generic) 120 actuation, and beclometasone (Ivax) 200 actuation] was weighted . using a laboratory scale (Sartorius R200D; 0.01g accuracy). Two of each pMDI were weighed after each actuation, with a 30-second inter-puff interval, and the mean weight was recorded. To minimise variability in measurements, weighing was limited to one operator. The canister was considered empty when there were no changes in weight after repeated actuation. The prediction equation (one for each pMDI) was the line of best fit through data points on the scatter plot of the number of actuations versus weight.RESULTS AND DISCUSSION There was low variability between pMDIs weights (SD: 0.03g-0.08g)] of the same active ingredients indicating manufacturing uniformity among canisters. Prediction equations were generated for each type of active ingredients, where the general equation is: Actuation remaining = Constant + β*pMDI weight.CONCLUSION This study produced a prediction equation that can be used to estimate remaining actuation in a pMDI based on its weight. Weighing medication canister could be used to measure actuation remaining in pMDIs, as well as patients’ adherence to pMDIs.
Biomarkers of Healthy and Diseased Pulp and Periapical Tissue: A Review
Authors: Jain Atul , Aurwade Vinod , Bahuguna Rachana , Agarwal Abhinay 
Published: October 2020

Establishing an accurate diagnosis is the first and prime requisite, in initiating the correct treatment, but unfortunately due to the limitations of the diagnostic procedure, this step can be highly confounding. Biological marker serves as a parameter, that is indicative of underlying physiology and health of the tissue. Healthy and diseased pulp and periapical tissue, tend to reflect physiologic and pathologic agents, which on isolation and estimation, can help diagnose, the condition more conclusively. Use of these agents, along with signs, symptoms, result of various tests, can aid in establishing the condition, more precisely. This review article focuses upon, identification of biomarkers, that play important role in pathogenesis of dental caries, pulpal and periapical pathosis and help to diagnose the pathological and healthy condition accurately, thus guide decisions, regarding clinical treatment.
Key words: biological products, biomarkers, dental Caries, dental Pulp, diagnostic Tests
Concepções e práticas docentes
Authors: Zorzi Analisa , Aragón Rosane , Boff Turchielo Luciana 
Published: October 2020

Este artigo analisa concepções e práticas pedagógicas de dois cursos de Pedagogia, realizados na modalidade a distância. A pesquisa fundamenta-se na discussão teórica sobre currículo na formação de professores, considerando as perspectivas de Sacristán (2000), Mckernan (2009) e Nóvoa (2017). Os dados deste estudo qualitativo são as produções escritas e publicadas sobre os cursos na forma de artigos, capítulos de livros e dissertações de mestrado, disponíveis em repositórios digitais com acesso on-line. Uma análise de conteúdo foi realizada com base em categorias que permitiram mapear e compreender os modelos de formação “em ação”, destacando-se como as especificidades dos currículos sustentaram as aprendizagens dos estudantes. Os resultados evidenciam que a articulação teoria e prática, elemento central nos projetos pedagógicos dos cursos, foi efetivada (currículo real) mediante uma estratégia de formação baseada em análises e reflexões sobre as experiências dos alunos em seus espaços de docência, favorecendo os processos de aprendizagem.