bluuebunny/crossref_metadata_2025_split huggingface dataset:
The quality of Sumba Ongole sperms after sexing using bovine serum albumin column in Bracket Oliphant extender at different temperatures
Authors: Agasi F A , Yusuf M , Said S , Toleng A L 
Published: June 2020

Abstract
The objective of this study was to determine the effect of using Bracket Oliphant medium (BO) extender at different temperatures on motility, viability, abnormalities and plasma membrane integrity of sexed sperms using fresh semen of Sumba Ongole (SO). Sexing sperms was carried out using a BO extender in the BSA column. The parameters observed in this study were motility, viability, abnormalities and plasma membrane integrity and were compared using the Paired-Sample t-Test with two treatments and six replications. P1 was BO extender with a temperature of 27°C and P2 was BO extender with a temperature of 37°C. The results of this study showed that there were no significant different (P>0.05) between the two treatments of the sperms X for motility (65.8%, 53.3% and 41.3% vs. 66.3%, 55.0% and 43.3%), viability (65.9%, 68.0% and 67.0% vs. 67.7%, 61.9% and 69.6%) and plasma membrane integrity (70.1% and 59.8% vs. 64.9% and 64.2%) after sexing, equilibration and thawing, but differed significantly (P<0.05) in abnormalities after sexing (15.5%, 15.6% and 16.2% vs. 15.5%, 17.3% and 19.5%). Likewise, the motility (66.7%, 55.8% and 42.1% vs. 65.8%, 55.0% and 42.1%), viability (68.8%, 59.0% and 64.5% vs. 67.4%, 59.4% and 71.7%), abnormalities (13.6%, 19.4% and 16.6% vs. 18.9%, 18.6% and 17.2%) and plasma membrane integrity (68.1% and 57.5% vs. 62.8% and 62.8%) of sperms Y at P1 and P2 did not differed significantly (P>0.05). The difference in the value of abnormalities of the sperms after separation is still within the limits of abnormalities. It can be concluded that the use of the BO extender method can be used at a temperature of 27°C and at 37°C in the process of sexing sperms.
The quality of sexed sperm separated using bovine serum albumin column and extended using tris aminomethane at different temperatures
Authors: Teken G E , Yusuf M , Said S , Toleng A L 
Published: June 2020

Abstract
Sexing sperms is one of the reproductive technologies that is considered as a promising alternative in the effort of reproductive efficiency to produce offspring according to the desired sex. The method for maintaining and extending the sperms can be done by using bovine serum albumin (BSA) column with Tris Buffer at room temperature and waterbath without damaging the quality of the sperms. The purpose of this study was to determine the effect of BSA - Tris Buffer extender on the quality of sexed sperms after thawing at 27°C and 37°C. The study was divided into two treatments; P1 was Tris extender in the temperature of 27°C and P2 was Tris extender in the temperature of 37°C with 6 replications. Those treatments were compared using a Paired Sample test (T-Test). The parameters observed in the present study were motility, viability, abnormalities, and intact plasma membrane. The results showed that motility, viability, abnormalities, and intact plasma membrane of the sperms X and Y at 27°C and 37°C after sexing did not differed significantly (P>0.05). Likewise, after equilibration, the motility of sperms X at 27°C and 37°C did not differed significantly (P>0.05), however, sperm Y at 27°C and 37°C showed a significant difference (P<0,05). Viability and abnormalities of sperms X at 27°C and 37°C differed significantly (P<0,05) but sperms Y at 27°C and 37°C did not show any significant differences. After thawing process, motility, viability, abnormalities and intact plasma membrane of the sperms X and Y at 27°C and 37°C did not show any significant differences. However, it can be conluded that the quality of sexed sperms in the present study is still good because it has motility above 40% and abnormalities below 20%.
Maturation of sheep oocytes with antioxidant α-tocopherol which are activated by parthenogenesis
Authors: Gustina S , Hasbi H , Supriatna I , Setiadi M A 
Published: June 2020

Abstract
This study was conducted to investigate the competence of sheep oocytes which are matured with antioxidant α-tocopherol by parthenogenesis activation. Oocytes from slaughterhouse were matured in tissue culture medium (TCM)-199 supplemented with 10% fetal bovine serum (FBS), 10 IU/ml pregnant mare serum gonadotrophin (PMSG), 10 IU/ml human chorionic gonadotrophin (hCG), 1 µg/ml estradiol, 50 µg/ml gentamycin, and with or without 150 µM α-tocopherol supplementation, then were parthenogenetically activated by exposing to 5 µM calcium ionophore for 5 min, and in medium containing 5 µg/ml cytochalasin B for 4 h. Furthermore oocytes were cultured in TCM 199 + 10% FBS either with or without supplementation of 150 µM α-tocopherol. Result of this experiment revealed that the percentages of activated oocytes with supplementation of 150 µM α-tocopherol only in maturation medium (63.41%) significantly higher (P<0.01) than the control group (50.00%) and the supplemented only in culture medium (51.02%). Furthermore, no significantly difference (P>0.05) in the number of oocytes with 1 pronucleus (PN), 2 PN, and more than 2 PN. It is concluded that the supplementation of 150 µM α-tocopherol on the maturation medium could also increase the percentage of activated oocytes optimally.
Increasing milk production and milk chemical composition in dairy cows by a supplementation of Moringa
oleifera leaf (mol) powder block
Authors: Rika N , Toleng A L , Yusuf M , Sahiruddin  , Ako A 
Published: June 2020

Abstract
Lower milk productivity of dairy cows might be correlated with the low milkconsumption in Indonesia. This problem might be adversely affected by low quality and quantity of food intake. Therefore, the purpose of this study was to evaluate the milk production of dairy cows supplemented with “Moringa oleifera leaf (MOL) powder block”. There were 20 suckling Friesh Holland dairy cows used in this study. All animals were fed with roughage of natural elephant grasses and rice bran. They were divided into two groups of treatments. The first group supplemented with MOL powder block 500 g/head/day for 21 days and the second group has remained without a supplementation. Hand milking was performed twice a day. Daily milk production and milk concentrations of fat, protein, lactose, calcium and phosphorus were recorded. The data were compared, between the two treatments by using student’s t-test. The supplementation of MOL powder block significantly (p<0.05) increased milk production compared to that without supplementation (14.25 vs 11.51 litre/head/day). Protein in milk was significantly (p<0.05) higher in the treated group in compared to that in control group (3.89 vs 3.43 %, respectively). This study concluded that milk production and protein concentration in milk could be increased by supplementation of MOL powder block.
Reducing heat stress of Bali cattle under a tropical condition by water sprinkler
Authors: Toleng A L , Yusuf M 
Published: June 2020

Abstract
Although Bali (native breed of Indonesia) cattle has already adapted to tropical conditions, but this breed was reported to be in heat stressed under the high ambient temperature. This study was conducted to evaluate the effect water sprinkler on the physiological responses of Bali cattle during the hot season. Two groups of 6 young Bali males were used in this study. The animals were kept individually in an animal house and fed with rice straw and native grasses supplemented with rice bran and urea multinutrients molasses block. The first group (control group) was kept without water sprinkler and the second group (treatment group) was treated with an individual water sprinkler located about 1 m above the animal. The water sprinklers were applied for 6 consecutive days, three times a day (8.00, 12.00 and 16.00 hr.) each lasting for 30 minutes. The physiological responses (body temperature, respiration rate and pulse rate) and ambient temperature were measured at 05.00, 09.00, 13.00, 17.00, 21.00 and 01.00 hr. Daily water intake was recorded. Mean ambient temperature at 05.00, 09.00, 13.00, 17.00, 21.00 and 01.00 hr. were 20.50º, 32.17º, 37.33º, 30.00º, 21.67º, and 20.33ºC, respectively. Treatment group showed a significant (P<0.05) lower mean body temperature during day time (09.00, 13.00 and 17.00 hr.) in compared to those in the control group (38.1ºC vs 38.5ºC). During the night (21.00, 01.00 and 05.00 hr.), there were no significant different of mean body temperature between the two treatments (38.0ºC vs 38.1ºC). Similar responses were detected for respiration rate and pulse rate. Respiration rate was significantly lower (P<0.01) during day time in the treatment group in compared to that in the control group (18.1 vs 20.8 breaths/minute). Mean pulse rate at 13.00 and 17.00 hr. in the treatment was significantly lower (P<0.01) in compared to that in control group (64.3 vs 70.2 beats/min). Mean daily water intake was significantly (P<0.01) lower in the treatment group in compared to that in the control group (12.1 vs 19.3 liters/day). It can be concluded that high temperature during day time of the hot season induced a heat stress. The application of water sprinkler could effectively reduce the heat stress in Bali cattle.
Estrous and pregnancy rate responses of postpartum Bali cattle to concentrate supplementation with different protein levels of rice-straw as basal ration
Authors: Sonjaya H , Rahim L , Sari D K , Abdullah A , Gustina S , Hasbi H 
Published: June 2020

Abstract
The aim of the research is to determine the effect of concentrate with different protein level rice straw as basal ration on the percentage of pregnancy rate of Bali cattle maintained on traditional farming system. The research was divided into two experiments. First experiment: a number of 46 cows divided into 4 treatment groups. Each group respectively protein level 0%, 10%, 12%, and 14%. Experiment 2: a number of 324 Bali cows postpartum got concentrate supplementation with different protein levels were divided into three groups, each got a protein level of 10%; 12%, and 14%. The rice straw diet given ad-libitum and concentrate as much as 1 kg per head per day for two months. Livestock that appears estrous after feeding directly mated naturally and partially performed artificial insemination. Pregnancy examinations carried rectal palpation and partly validated with ultrasound. The results showed that estrous respon in the first experiment not significantly affected by supplementation of concentrate with protein level for 10–14%. In contrast, estrous intensity was obviously visible in group treatment with 14% protein level. The supplementation of protein level in concentrate increased natural estrous 66.05% and natural mating generated 91.65% of pregnancy rate. However, 43.95% cows were artificially inseminated generating 81.63% of pregnancy rate. It’s sonclude that supplementation of concentrate for feed Bali cows with rice straw as basal in the dry season caused increasing the percentage of postpartum estrous and highest of pregnancy rate.
The effect of heatsynch protocol on repeat breeding dairy cows
Authors: Ramadhanty D , Yusuf M , Toleng A L , Rahardja D P , Sahiruddin  , Mansur M , Fausiah A 
Published: June 2020

Abstract
The aim of this study was to know the incidence of repeat breeding in dairy cows and to know the effectiveness of Heatsynch protocol on dairy repeat breeding cows. The study was conducted in two stages. The first stage, all dairy cows were recorded and clinically examined through palpation per rectum and for their reproductive statuses. Parameter measured in the study was the incidence of repeat breeding. The second stage of the study was that the repeat breeder cows subjected to Heatsynch protocol for estrus induction. The protocol was GnRH injection on day-0, followed by PGF2α, estradiol injections on day-7 and day-8, respectively, and inseminated artificially on day-9. The results of the study indicated that the incidence of repeat breeding was 41.6%. The Heatsynch protocol was successfully increase the number of pregnant repeat breeder cows; 75%, higher compared to untreated repeat breeder cows; 20%. It can be concluded that high incidence of repeat breeding in dairy cows. Heatsynch protocol can be used to treat repeat breeder cows and improve the reproductive efficiency.
Supplementation of Arabian jujube (Ziziphus spina Christi) leaf extracts as extender material on the quality of Bali bull semen
Authors: Sahiruddin  , Widjiati  , Toleng A L , Yusuf M , Masturi  
Published: June 2020

Abstract
Semen is subject to experience a decrease in the quality including motility of the sperms. Decreasing the quality of semen begins during collecting process and continues at the processing stage. Low quality of semen will have an impact on the low fertilization rate, so that efforts are needed to maintain the quality of semen. This is thought to be caused by oxidative reactions that are triggered by free radical activity. Some active ingredients in the Arabian jujube leaf are thought to function as natural antioxidants. This study aimed to determine the effect of supplementation of Arabian jujube leaf extracts (Ziziphus spina Christy) on sperms motility in an effort to maintain the quality of Bali bull fresh semen. This study was conducted at the Laboratory of Animal Reproduction; Semen Processing Unit, Faculty of Animal Science, Hasanuddin University, Makassar. The Arabian jujube leaf extracts was added in the extender at three different treatments; P1 (1%), P2 (3%), P3 (5%) and P0 (control). The results of this study showed that there were different motility of the sperms after supplementing the extender with Arabian jujube leaf extracts. The sperms motility of Bali bull semen at concentration of 5% during five days equilibration at 5°C had higher than 3%, 1%, and control, respectively (50.0 vs. 48.7, 43.8, and 46.3%). It can be concluded that supplementation of Arabian jujube leaf extract in the extender material could maintain the quality of Bali bull semen.
Ecology and adaptation of legumes crops: A review
Authors: Al-Tawaha A R M , Al-Tawaha A , Sirajuddin S N , McNeil D , Othman Y A , Al-Rawashdeh I M , Amanullah  , Imran  , Qaisi A M , Jahan N , Shah M A , Khalid S , Sami R , Rauf A , Thangadurai D , Sangeetha J , Fahad S , Youssef R A , Al-Taisan W A , Al-Taey D K A 
Published: June 2020

Abstract
In this review, we discuss the relationship between environmental crop management and adaptation to warming climates of legume plants and plant breeding, for drought tolerance. The benefits connected to the impact of the expansion of appropriate legumes-based agriculture for arid zones are analyzed. We need to two aims to develop programs of breeding and management guided to drought tolerance. The first one is enhancing productivity. The second one is to improve the drought tolerance by mechanisms of water conservation.
Livestock waste and its role in the composting process: A review
Authors: Said M I 
Published: June 2020

Abstract
Livestock waste, especially manure, is one source of pollution that needs attention. Increasing the number of the livestock industry will encourage increased livestock waste. An increase in livestock population will be directly proportional to waste production. Various efforts have been made to reduce the production of such waste. Along with technological advances, efforts to reduce livestock waste have been carried out such as biogas production, compost processing and bioenergy production. In Indonesia, composting is a simple technology that has developed. The use of a mixture of animal manure with materials from agricultural waste has been widely developed. The combination of these two ingredients will produce a better quality compost product. The application of microorganisms as decomposers has been widely developed in the process of composting livestock waste. Structurally, decomposers will increase and accelerate the process of degradation of compost raw material components. The effectiveness of microorganisms is influenced by the environment. The description in this paper aims to evaluate the role of waste from animal manure as a raw material in the composting process.
Quality of compost produced from different types of decomposer substrate and composition of straw
Authors: Said M I , Hastang  , Isra V N 
Published: June 2020

Abstract
Compost is one of the processes of decomposing organic materials that involves the activity of microorganisms partially. The use of decomposers is very important to accelerate the rate of compost fermentation. The study was aims to evaluate the quality of compost produced from different types of decomposer substrates and straw compositions. This study uses feces from Bali cattle as main material which are maintained in a semi-intensive manner. Two types of decomposer substrate were used, namely (1) animal substrate (AS) and (2) plant substrate (PS). The level of straw applied were (1) 0%, (2) 5% and (3) 10% (from the total of compost). The study was conducted based on a completely randomized design (CRD) factorial pattern. The data were analyzed using ANOVA. The results showed that differences in the type of decomposer substrate and straw composition had no significant effect (P>0.05) on the pH value, levels of C-organic, N-organic and C/N ratio of compost. The pH value is in the range of 7.40-7.69. The value of C-organic content varies with a range of values 15.29-18.52%. N-organic content is in the range of 0.72 to 0.97% and C / N ratio of 19.00-25.66. The final results of the study showed that the use of animal substrate decomposer and using 10% straw from the from the total of compost showed better compost quality compared to other treatments.
Analysis of C-organic and total Phospor (P2O5) contents of soil around egg-laying chicken farm
Authors: Abdullahi A B , Siregar A R , Pakiding W , Mahyuddin  
Published: June 2020

Abstract
Sustainable animal husbandry development should be environmentally friendly, meaning it doesn’t cause pollution and degradation of environmental quality, i.e. technically efficient, economically fit for use, socially acceptable and ecologically still ensuring the balance of other ecosystems. One of the resources which should be noted is the soil condition around the farm which is suspected to have degraded quality due to pollutions caused by farming activities. Based on that, the environmental test was performed by analyzing the C-Organic and Total Phosphor (P2O5) contents of soil around the egg-laying chicken farm. The present study was aimed to (1) know the C-Organic and P2O5 contents of soil around the egg-laying chicken farm; (2) compare the C-Organic and P2O5 contents of soil around the egg-laying chicken farm with the set assessment criteria for soil chemical properties (C-Organic and P2O5) for farmland. The present study was performed on October-December 2018, soil samples were obtained from locations around egg-laying chicken farms in Sidenreng Rappang Regency, South Sulawesi, Indonesia. The method used in testing the C-Organic and P2O5 contents of soil was spectrophotometric. The working procedure in the present study: (1) testing the C-Organic and P2O5 contents of soil in the location and around egg-laying chicken farm; (2) comparing the C-Organic and P2O5 contents of soil from the research result with the set assessment criteria of soil chemical properties (C-Organic and P2O5) for farmland. The research result showed that the average C-Organic content of the soil around the egg-laying chicken farm was 0.699% and the average P2O5 content of the soil around the egg-laying chicken farm was 159.2 mg/100 g. Based on the results, it’s concluded that the content of C-Organic soil around the egg-laying chicken farm is very low because < 1.0 % but P2O5 very high because of > 60 mg/100 g.
Greenhouse Gas Emission (GHG) from ruminant on 2016 at Central Sulawesi Province
Authors: Munier F F , Wardi  , Takdir M 
Published: June 2020

Abstract
Ruminant is the one of sectors livestock that contribute to increase global warming originates manure and animal eructation. Ruminant sector accounted for carbon dioxide (CO2), methane (CH4), nitrous oxide (N2O), and ammonia that caused the acid rain. The objective of this research was to determine the contribution of livestock sector for greenhouse gas emission (GHG) on 2016 at Central Sulawesi. The research was used ALU Tool software to calculate the emission of GHG. Data source were from livestock population and emission factors (EF) of CH4 and N2O of any livestocks. The results showed that Central Sulawesi Provincecontribute to 574.020 (Gg CO2e) emission a year from ruminant. Big Rumintant or Beef cattle is the main contributor on GHG emission in form of enteric fermentation CH4 453.550 (Gg CO2e), as much as 76.42%. Small rumintant is the production of N2O from goats as much as 106.021 (Gg CO2e) or equivalent to 17.12 %.
Population dynamics of the white spotted rabbitfish (Siganus canaliculatus Park, 1797) in Makassar Strait and Gulf of Bone, Indonesia
Authors: Suwarni  , Tresnati J , Omar S B A , Tuwo A 
Published: June 2020

Abstract
The white spotted rabbitfish (Siganus canaliculatus Park, 1797) is a locally important foodfish across much of Indonesia. This study aimed to support population dynamics-based fisheries management of white spotted rabbitfish stocks in the Makassar Strait and Gulf of Bone through providing data on age classes (cohorts), growth, mortality, and exploitation rate. Field data were collected over the year from February 2017 to January 2018. A total of 2248 white spotted rabbitfish (1810 males and 438 females) were collected from the Makassar Strait and 1686 (1277 males and 409 females) from the Gulf of Bone. The fish were measured (L = total length in mm), and all analyses were implemented in FISAT II. Cohorts were determined by sex based on monthly length-frequency data using the Bhattacharya method. The growth factor K and asymptotic length L∞ of white spotted rabbitfish in the Makassar Strait were K = 0.42/yr and L∞ = 211.98 mm with t0 = -0.250 yr for males and K = 0.43/yr, L∞ = 215.00 mm and t0 = -0.386 yr for females. In the Gulf of Bone the values were K = 0.42/yr, L∞ = 211.98 cm and t0 = -0.250 yr for males and K = 0.43/yr, L∞ = 215.00 mm and t0 = -0.386 yr for females. Mortality parameters of white spotted rabbitfish in the Makassar Strait were total mortality Z = 1.70/yr, natural mortality M = 0.76/yr, F = 0.94/yr giving an exploitation rate of E = 0.55 for males, while for females Z = 1.77/yr, M = 0.84/yr, F = 0.93/yr and E = 0.53. In the Gulf of Bone, for male white spotted rabbitfish Z = 1.78/yr, M = 0.60/yr, F = 1.18/yr and E = 0.67, while for females Z = 2.42/yr, M = 0.60/yr, F = 1.82/yr and E = 0.75/yr. These data indicate heavy fishing of both stocks, most likely at unsustainable levels.
The diversity of ectoparasites on some type of rats
Authors: Sepe M , Maryana N , Priyambodo S 
Published: June 2020

Abstract
The rats were parasitized by ectoparasitic arthropods which have a close relationship with rats as their specific host. In the rat’s body found various types of ectoparasites such as ticks, mites, fleas, and ticks at the same time and is known as polyparasit. This research aims at obtaining data about the diversity of ectoparasites on different types of rats and their habitat. The rats caught in the house habitat, forest, field, and sewer. The caught rats were put into a cloth bag, labeled, then taken to laboratory to be identified by using key rodent identification. Then, the researcher did the collection and identification. The results showed that There were 87 rats caught from all the habitats. The four rat species trapped from four habitats were Rattus rattus diardii, R. tiomanicus, R. argentiventer, and R. norvegicus. The total number of 2548 ectoparasites were collected from the rats of Hoplopluera pacifica, Polyplax spinulosa, Xenopsylla cheopis, Laelaps nuttalli Hirst and L. echidninus. The diversity index of ectoparasites on the bodies of R. tanezumi was predominant (0.525). Statistical analysis with t-tests (α = 5%) of all index diversity between male and female rodents infested with ectoparasites was not significantly different.