bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of prognostic and bone metastatic alternative splicing signatures in bladder cancer
Authors: Huang Runzhi , Zhang Jiayao , Wang Tianjing , Jia Jingyi , Song Dianwen , Yan Penghui , Yin Huabin , Hu Peng , Zhu Xiaolong , Huang Zongqiang , Meng Tong , Zhang Jie 
Published: March 2020

Abstract
Background ： Bladder cancer, originating from the epithelium of the urinary bladder, was the second most common malignancy in the urinary system with a high metastasis rate and poor post-metastasis prognosis. Alternative splicing events (ASEs) were regarded as important markers of tumor progression and prognosis, however, their roles in bladder cancer bone metastasis haven’t been recognized. Methods ： In order to explore the mechanism of ASEs in bladder cancer bone metastasis, we downloaded the RNA sequencing data and ASEs data of 412 samples of primary BLCAs from The Cancer Genome Atlas (TCGA) and TCGASpliceSeq databases. The Cox regression analysis was used to identify overall survival-related ASEs (OS-SEs), then, based on the OS-SEs screened by Lasso regression, we constructed the predict model. Finally, univariate and multivariate independent prognostic analysis were performed to prove it as an independent prognostic factor. Results ： In this study, a predict model of OS in BLCA was constructed and the Area Under Curve of the model was 0.581. Its risk score was also proved to be an independent predictor with the good accuracy (P < 0.001). Among identified 390 SFs, Junction plakoglobin (JUP) was significantly correlated with overall survival and bone metastasis. In co-expression analysis, the co-expression pathway of ITGB4 was the glycosphingolipid biosynthesis ganglio series. Conclusions ： We speculated that JUP regulating the ITGB4 might play a key role in bone metastasis of bladder cancer through the glycosphingolipid biosynthesis ganglio series pathway (R = 0.220, P < 0.001), which was also related to prognosis.
Preconditioning with rHMGB1 ameliorates lung ischemia–reperfusion injury through inhibiting alveolar macrophages pyroptosis via Keap1/Nrf-2/HO-1 signal pathway
Authors: Fei Lin , Jingyuan Xiao , Fangte Liang , Huijun Dai , Liu Ye , Ren Jing , Jinyuan Lin , Pan Linghui 
Published: March 2020

Abstract
Background Lung ischemia-reperfusion injury (LIRI) is a common and complex pathophysiological process that can lead to poor patient outcomes. Inflammasome-dependent macrophage pyroptosis contributes to organ damage caused by ischemia-reperfusion (I/R). Oxidative stress reaction and antioxidant enzymes also play an important role in LIRI. This experiment was conducted to investigate whether preconditioning with rHMGB1 could ameliorate LIRI and explore the mechanisms of its protective effect in a lung I/R mice model. Methods Adult male mice were anesthetized and the left hilus pulmonis was clamped for 60 min, followed by 120 min of reperfusion. rHMGB1 was performed by intraperitoneal injection at 2 h before anesthesia. Brusatol (Nrf-2 antagonist) was given intraperitoneally every other day for a total of five times before surgery. Measurements of pathohistological lung tissue damage, pulmonary wet/dry (W/D) ratios, inflammatory mediators were performed to assess the extent of lung injury after I/R. Alveolar macrophages (AMs) pyroptosis were evaluated by LDH release, caspase-1 expression in flow cytometry, GSDMD expression in immunofluorescent staining. Measurement of the products of oxidative Stress (ROS, MDA, 15-F2t-Isoprostane) and the antioxidant enzymes (SOD, GSH-PX, CAT) were performed. Results Preconditioning with rHMGB1 significantly ameliorated I/R-induced lung injury through measuring the morphology, wet/dry weight ratio, the expressions of IL-1β, IL-6, NF-κB, HMGB1 in lung tissue. rHMGB1 preconditioning remarkably alleviated AMs pyroptosis induced by lung I/R. rHMGB1 preconditioning significantly reduced oxidative stress and restored the activity of antioxidative enzymes. In addition, rHMGB1 preconditioning mediated the activity of Keap1/Nrf-2/HO-1 pathway in LIRI. Furthermore, inhibiting Keap1/Nrf-2/HO-1 pathway through brusatol administration could aggravate lung tissue damage and inflammatory response after lung I/R. Also, brusatol administration could suppresse the antioxidant and anti-pyroptosis effects of rHMGB1 preconditioning in LIRI. Conclusions rHMGB1 preconditioning protects against LIRI through suppressing AMs pyroptosis. The mechanism is partially explained by inhibiting oxidative stress and improving the activity of antioxidative enzymes via Keap1/Nrf-2/HO-1 pathway.
Targeting the MALAT1-miR-206-GLS axis enhances the apoptotic effects of gastric cancer cells by mild hyperthermia treatments
Authors: Shi Huijuan , Li Kejun , Feng Jinxin , Liu Gaojie , Feng Yanlin , Zhang Xiangliang 
Published: March 2020

Abstract
Background: To explore the molecular mechanisms and modulatory effects of long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in the hyperthermia-induced gastric cancer cell death. Methods: Combined qRT-PCR and GLS activity detection of glutamine metabolism, we demonstrate the difference of expression of MALAT1 and miR-206 in normal or cancer tissue/ several cell lines and the roles of two in regulation of glutamine, respectively. The relation of MALAT1 and miR-206 was research by bioinformatical analysis and luciferase report. In addition, the molecular regulation between apoptotic effects and MALAT1-miR-206-GLS axis in gastric cancer after mild hyperthermia treatments were discovered by western blotting, luciferase report, quantitative real-time PCR (qRT-PCR), GLS activity detection of glutamine metabolism and cell viability test. Results: MALAT1 positively associated with GC by promoting glutamine metabolism under short time hyperthermia treatment, it involved in an adaptive reaction, an increased glutamine metabolism rate which could be further overridden by long time hyperthermia exposures. miR-206 could inhibit glutamine metabolism of GC and target 3’UTR of GLS which has oncogenic functions in GC directly. In addition, miR-206-suppresed glutamine metabolism was through direct targeting GLS. LncRNA MALAT1 inhibited miR-206 expression in GC by sponging it as a competing endogenous RNA. In summary, MALAT1-promoted glutamine metabolism was through the miR-206-GLS axis. Furthermore, we demonstrated effective approaches from the MALAT1-miR-206 axis for enhancing the mild hyperthermia-induced cancer death under a short time. Conclusions: Taken together, the MALAT1-miR-206-glutamine metabolism axis is a new strategy for enhancing the selectivity of hyperthermia treatments specific to GC cells
Analysis of Emergency Department Visits for All Reasons by Adults with Depression in the United States
Authors: Hill Tyler , Jiang Yun , Friese Christopher , Darbes Lynae , Blazes Christopher K. , Zhang Xingyu 
Published: March 2020

Abstract
Background : We aimed to characterize Emergency Department (ED) utilization and outcomes of patients with depression seeking emergency care for all reasons. Methods : Using 2014–2016 ED data from the National Hospital Ambulatory Medical Care Survey, we investigated demographics, ED resource utilization, clinical characteristics, and disposition of patients with depression versus those without depression. Results : Approximately 10,626,184 (11.4%) out of 92,899,685 annual ED visits were by patients with depression. ED patients with depression were mostly non-Hispanic White (74.0%) and were less likely to be male than patients without depression (aOR: 0.56; [95%] CI: 0.56–0.56). ED patients with depression were more likely to be admitted to the hospital (aOR: 1.56; CI: 1.55–1.56) and intensive care unit (ICU) (aOR: 1.28; CI: 1.27–1.28) than patients without depression. Among ED patients with depression, males were more likely than females to be seeking emergency care for psychiatric reasons (aOR: 2.04; CI: 2.03–2.05) and to present with overdose/poisoning (aOR: 1.35; CI: 1.34–1.36). Conclusions : We described the unique demographic, socioeconomic, and clinical characteristics of ED patients with depression, using the most comprehensive, nationally representative study to date. We revealed notable gender disparities in rates and reasons for admissions. The higher hospital and ICU admission rates of ED patients with depression suggests this population requires a higher level of emergency care, for reasons that remain poorly understood.
Changes in 3-month mineral and bone disorder patterns were associated with all-cause mortality in prevalent hemodialysis patients with secondary hyperparathyroidism
Authors: Kato Chihiro , Fujii Naohiko , Miyakoshi Chisato , Asada Shinji , Onishi Yoshihiro , Fukuma Shingo , Takanobu Nomura , Michihito Wada , Fukagawa Masafumi , Fukuhara Shunichi , Akizawa Tadao 
Published: March 2020

Abstract
Background. There is limited evidence on the association between short-term changes in mineral and bone disorder parameters and survival in maintenance hemodialysis patients. Methods. We investigated the association between changing patterns of phosphorus, calcium and intact parathyroid hormone levels and all-cause mortality in hemodialysis patients with secondary hyperparathyroidism. Each parameter was divided into three categories (low [L], middle [M] and high [H]), and the changing patterns between two consecutive visits at 3-month intervals were categorized into nine groups (e.g., L-L and M-H). The middle category was defined as 4.0-7.0 mg/dL for phosphorous, 8.5-9.5 mg/dL for calcium and 200-500 pg/mL for intact parathyroid hormone. Adjusted incidence rates and rate ratios were analyzed by weighted Poisson regression models accounting for time-dependent exposures. Results. For phosphorus, shifts from low/high to middle category (L-M/H-M) were associated with a lower mortality compared with the L-L and H-H groups, whereas shifts from middle to low/high category (M-L/M-H) were associated with a higher mortality compared with the M-M group. For calcium, shifts from low/middle to high category (L-H/M-H) were associated with a higher mortality compared with the L-L and M-M groups, whereas shifts from high to middle category (H-M) were associated with a lower mortality compared with the H-H group. For intact parathyroid hormone, shifts from low to middle category (L-M) were associated with a lower mortality compared with the L-L group. Conclusions. Changes in the 3-month patterns of phosphorus and calcium toward the middle category were associated with lower mortality. Our study also suggests the importance of avoiding hypercalcemia.
Generation and validation of a highly sensitive bioluminescent HIV-1 reporter vector that simplifies measurement of virus release
Authors: Kirui James , Freed Eric 
Published: March 2020

Abstract
Background The continued persistence of HIV-1 as a public health concern due to the lack of a cure calls for the development of new tools for studying replication of the virus. Here, we used NanoLuc, a small and extremely bright luciferase protein, to develop an HIV-1 bioluminescent reporter virus that simplifies functional measurement of virus particle production. Results The reporter virus encodes a Gag protein containing NanoLuc inserted between the matrix (MA) and capsid (CA) domains of Gag, thereby generating virus particles that package high levels of the NanoLuc reporter. We observe that inserting the NanoLuc protein within HIV-1 Gag has minimal impact on Gag expression and virus particle release. We show that the reporter virus recapitulates inhibition of HIV-1 particle release by Gag mutations, the restriction factor tetherin, and the small-molecule inhibitor amphotericin-B methyl ester. Conclusion These results demonstrate that this vector will provide a simple and rapid tool for functional studies of virus particle assembly and release and high-throughput screening for cellular factors and small-molecules that promote or inhibit HIV-1 particle production.
Performance evaluation of combined ultraviolet-ultrasonic technologies in removal of sulfonamide and tetracycline resistant Escherichia coli from domestic effluents
Authors: Rebecca Annisha Owassa Dza , Li Zifu , Zhou Xiaoqin , Madgil Don Stenay Ngomah , Donde Oscar Omondi 
Published: March 2020

Abstract
Proper treatment of wastewater is key to the achievement of sustainable environmental management. The use of ultraviolet radiation and ultrasound have continued to be considered as some of the best sustainable practices in wastewater purification. However, despite the suitability of the two emerging techniques in sustainably increasing the purification efficiencies of wastewater, their application has not been fully understood, especially in eliminating faecal pathogenic microorganisms. Moreover, their combined potential in the elimination of Escherichia coli resistant genes from wastewater has not been adequately explored. This study was designed to evaluate the potential of individual and combined/integrated ultraviolet radiation and ultrasonic technologies in the removal of antibiotic-resistant E. coli from domestic effluents. There was a statistical difference in the mean log units of sulfonamide resistant E. coli between the different ultraviolet radiation and ultrasonic dosages (P &lt; 0.05), showing that ultraviolet radiation technology was more effective in the removal of both sulfonamide and tetracycline resistant E. coli from the wastewater. However, the integrated ultraviolet radiation-ultrasonic technique was highly efficient and is recommended in the removal of antibiotic resistant E. coli from wastewater. Nonetheless, further studies also need to be performed to further evaluate the disinfection effectiveness on a different bacteria species under continuous operation.
The Anti-osteoporosis Effects of Natto on Bone Mineral Density in Perimenopausal Women
Authors: Liu Yuan , He Yuan , He Baorong , Kong Lingbo 
Published: March 2020

Objective and Aims:
This study aims to conduct a systematic review and meta-analysis
for prospective studies to investigate the improvement effects of natto on bone mineral density
(BMD) in perimenopausal women.

Methods:
PubMed, EMBASE and Cochrane database searched upto February 2019. This study
was carried out according to the PRISMA guidelines10 for systematic reviews. The protocol of the
review was registered in the PROSPERO registry (CRD42019133183).

Results:
The review identified 3 unique prospective studies comprising 1658 non-overlapping participants.
Meta-analysis showed that natto could significantly improve lumbar bone mineral density
(BMD) (P=0.002, WMD=0.26; 95% CI:0.09-0.43) in cohort studies. However, the randomized
controlled study showed no statistical difference between the two (P=0.31, WMD=0.05; 95% CI:-
0.05-0.15). In addition, natto significantly improved the BMD of the femoral neck in a cohort
study and randomized control study (P=0.03, WMD=0.42). 95% CI:0.05-0.79, I2= 72%); (P<
0.0001, WMD=0.16; 95% CI:0.08-0.24), respectively. However, all studies demonstrated that
natto has no improvement effects on a hip joint (BMD). In that, the cohort study showed no statistical
significance between the natto intake group and the control group (P=0.21, WMD=0.10). 95%
CI:-0.06-0.25, I2= 18%) and the randomized controlled study also showed no statistical significance
between the natto intake group and the control group (P=0.09, WMD=-0.06). 95% CI:-0.13-
0.01).

Conclusion:
Through our current systematic review and meta-analyize of these prosepctive studies
of natto's anti-osteoporostic effecs on BMD, we found that the dietary intake of natto demonstrated
a improving effects on the BMD of the femoral neck, but has no effects on the hip joint. Such interesting
results may be related to the differences between anatomical structure between various
tissues. Besides that, the results of the RCT study and cohort study on the lumbar spine were not
the same, which may be related to the fact that participants in the RCT study were Caucasian and
participants in the cohort study were Asian. Therefore, more large-sample and high-quality RCT
studies are needed to further clarify the improvement effect of natto on osteoporosis.
Antioxidant Proteins’ Identification Based on Support Vector Machine
Authors: Xu Yuanke , Wen Yaping , Han Guosheng 
Published: March 2020

Background:
Evidence have increasingly indicated that for human disease, cell
metabolism are deeply associated with proteins. Structural mutations and dysregulations of these
proteins contribute to the development of the complex disease. Free radicals are unstable molecules
that seek for electrons from the surrounding atoms for stability. Once a free radical binds to an
atom in the body, a chain reaction occurs, which causes damage to cells and DNA. An antioxidant
protein is a substance that protects cells from free radical damage. Accurate identification of
antioxidant proteins is important for understanding their role in delaying aging and preventing and
treating related diseases. Therefore, computational methods to identify antioxidant proteins have
become an effective prior-pinpointing approach to experimental verification.

Methods:
In this study, support vector machines was used to identify antioxidant proteins, using
amino acid compositions and 9-gap dipeptide compositions as feature extraction, and feature
reduction by Principal Component Analysis.

Results:
The prediction accuracy Acc of this experiment reached 98.38%, the recall rate Sn of the
positive sample was found to be 99.27%, the recall rate Sp of the negative sample reached 97.54%,
and the MCC value was 0.9678. To evaluate our proposed method, the predictive performance of
20 antioxidant proteins from the National Center for Biotechnology Information(NCBI) was
studied. As a result, 20 antioxidant proteins were correctly predicted by our method. Experimental
results demonstrate that the performance of our method is better than the state-of-the-art methods
for identification of antioxidant proteins.

Conclusion:
We collected experimental protein data from Uniport, including 253 antioxidant
proteins and 1552 non-antioxidant proteins. The optimal feature extraction used in this paper is
composed of amino acid composition and 9-gap dipeptide. The protein is identified by support
vector machine, and the model evaluation index is obtained based on 5-fold cross-validation.
Compared with the existing classification model, it is further explained that the SVM recognition
model constructed in this paper is helpful for the recognition of antioxidized proteins.
Assessment of the Frequency of Online Searches for Symptoms Before Diagnosis: Analysis of Archival Data
Authors: Hochberg Irit , Allon Raviv , Yom-Tov Elad 
Published: March 2020

Background
Surveys suggest that a large proportion of people use the internet to search for information on medical symptoms they experience and that around one-third of the people in the United States self-diagnose using online information. However, surveys are known to be biased, and the true rates at which people search for information on their medical symptoms before receiving a formal medical diagnosis are unknown.

Objective
This study aimed to estimate the rate at which people search for information on their medical symptoms before receiving a formal medical diagnosis by a health professional.

Methods
We collected queries made on a general-purpose internet search engine by people in the United States who self-identified their diagnosis from 1 of 20 medical conditions. We focused on conditions that have evident symptoms and are neither screened systematically nor a part of usual medical care. Thus, they are generally diagnosed after the investigation of specific symptoms. We evaluated how many of these people queried for symptoms associated with their medical condition before their formal diagnosis. In addition, we used a survey questionnaire to assess the familiarity of laypeople with the symptoms associated with these conditions.

Results
On average, 15.49% (1792/12,367, SD 8.4%) of people queried about symptoms associated with their medical condition before receiving a medical diagnosis. A longer duration between the first query for a symptom and the corresponding diagnosis was correlated with an increased likelihood of people querying about those symptoms (rho=0.6; P=.005); similarly, unfamiliarity with the association between a condition and its symptom was correlated with an increased likelihood of people querying about those symptoms (rho=−0.47; P=.08). In addition, worrying symptoms were 14% more likely to be queried about.

Conclusions
Our results indicate that there is large variability in the percentage of people who query the internet for their symptoms before a formal medical diagnosis is made. This finding has important implications for systems that attempt to screen for medical conditions.
User Experiences of Social Support From Companion Chatbots in Everyday Contexts: Thematic Analysis
Authors: Ta Vivian , Griffith Caroline , Boatfield Carolynn , Wang Xinyu , Civitello Maria , Bader Haley , DeCero Esther , Loggarakis Alexia 
Published: March 2020

BackgroundPrevious research suggests that artificial agents may be a promising source of social support for humans. However, the bulk of this research has been conducted in the context of social support interventions that specifically address stressful situations or health improvements. Little research has examined social support received from artificial agents in everyday contexts.ObjectiveConsidering that social support manifests in not only crises but also everyday situations and that everyday social support forms the basis of support received during more stressful events, we aimed to investigate the types of everyday social support that can be received from artificial agents.MethodsIn Study 1, we examined publicly available user reviews (N=1854) of Replika, a popular companion chatbot. In Study 2, a sample (n=66) of Replika users provided detailed open-ended responses regarding their experiences of using Replika. We conducted thematic analysis on both datasets to gain insight into the kind of everyday social support that users receive through interactions with Replika.ResultsReplika provides some level of companionship that can help curtail loneliness, provide a “safe space” in which users can discuss any topic without the fear of judgment or retaliation, increase positive affect through uplifting and nurturing messages, and provide helpful information/advice when normal sources of informational support are not available.ConclusionsArtificial agents may be a promising source of everyday social support, particularly companionship, emotional, informational, and appraisal support, but not as tangible support. Future studies are needed to determine who might benefit from these types of everyday social support the most and why. These results could potentially be used to help address global health issues or other crises early on in everyday situations before they potentially manifest into larger issues.