bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSI-15 Determining the molecular mechanism through which estradiol and trenbolone acetate improve skeletal muscle growth in beef cattle
Authors: Reichhardt Caleb C , Bidwell Chris A , Thomas Aaron , Ahmadpour Amir 
Published: December 2019

Abstract
Approximately 90% of beef cattle on feed in the United States receive an anabolic implant during production, which results in increased growth, efficiency, and economic return to producers. However, the molecular mechanism through which these anabolic implants operate to improve skeletal muscle growth remains unknown. The objective of this study was to determine the molecular mechanism through which estradiol (E2) and trenbolone acetate (TBA) improve skeletal muscle growth in beef cattle and to specifically understand whether E2 and/or TBA function by modulating transcription. To address this, bovine satellite cells (BSC) were isolated from three different backgrounded steers and grown in culture. Once cultures reached 75% confluency they were treated with 1% fetal bovine serum (FBS) and 10 nM E2 or 10 nM TBA. In an additional experiment, all previously listed treatments were given in addition to actinomycin D (AD, a non-specific inhibitor of transcription). Treatment with E2 or TBA increased proliferation (P &lt; 0.05) when compared to a 1% FBS control. Furthermore, treatment with E2 or TBA in the presence of AD increased proliferation (P &lt; 0.01) when compared to cultures treated with just AD. These results indicate that TBA and E2 are both capable of increasing proliferation of BSC cultures through non-transcriptional mechanisms. Future work will identify the TBA and E2 signaling pathways that affect muscle growth and don’t involve changes in gene transcription.
PSVII-18 Nitrogen fertilization affects leaf-level sodium and potassium concentration in tall fescue pasture
Authors: Tooley Joshua A , Naumann Harley , Mendoza-Cozatl David G 
Published: December 2019

Abstract
Optimizing mineral supplementation is critical to maximizing production in a forage-based beef production system. The effect of nitrogen fertilization on mineral accumulation in tall fescue (TF) is not well understood. We evaluated the differences in mineral composition in nitrogen fertilized (TF+N) and unfertilized (TF-N) tall fescue pastures. The experiment was conducted at the University of Missouri Forage Systems Research Center during 2017 and 2018. A randomized complete block design was arranged in novel-endophyte TF pastures. All pastures were mechanically harvested in May. Nitrogen was applied to TF+N in June and again in August (45 kg ha-1 and 90 kg ha-1, respectively). Treatments were rotationally grazed from July to November using yearling cattle. Randomly selected 10 m2 strips were harvested biweekly and 300 g subsamples were dried. Nitric acid digestion was performed followed by ICP-MS to determine mineral content. Data were normalized using rank transformation and analyzed by treatment and month. Analysis of variance was conducted using PROC GLIMMIX (SAS 9.4); slice command was used to determine interactions within month by treatment and within treatment by month. Calcium and magnesium concentration decreased throughout the season with the lowest concentrations observed in both treatments during November (P = 0.0043 and P = 0.0007, respectively). Greater potassium concentrations were observed in June compared to November in both treatments (P &lt; 0.0334). We observed a within month by treatment interaction for sodium. Nitrogen fertilization resulted in greater sodium concentration in all months from August to November compared to TF-N (P &lt; 0.0074). Greater sodium concentrations resulted in decreased K:Na ratios during September, October, and November in TF+N (P = 0.0013, P = 0.0036, and P = 0.0024, respectively). Our results suggest nitrogen fertilization reduced K:Na ratio in TF pastures, which could negatively affect magnesium absorption in recticulo-rumen.
PSVII-14 Toxic and essential elements in seminal plasma and serum in stallions
Authors: Kalashnikov Valerii , Atroshchenko Michail , Zaitcev Aleksander , Miroshnikov Sergey , Zavjalov Oleg , Frolov Aleksey , Plemyashov Kirill , Krutikova Anna 
Published: December 2019

Abstract
The aim of the work was compare the concentration the of toxic and essential elements in the sperm plasma and blood serum of stallions. Sperm plasma and serum were collected from 39 Arabian stallions, aged 5 to 15 years. Concentration of toxic elements [aluminum (Al), arsenic (As), cadmium (Cd), lead (Pb), tin (Sn), mercury (Hg)] and essential elements (boron (B), lithium (Li), nickel (Ni), strontium (Sr) and vanadium (V)) in the sperm plasma and serum were determined by atomic emission and mass spectrometry (AES-ICP and MS-ICP) using an mass spectrometer Elan 9000 (Perkin Elmer, USA) and an atomic emission spectrometer Optima 2000V (Perkin Elmer, USA). It is established that the concentration of toxic and essential elements in the sperm plasma is higher than in serum. The greatest difference between the average concentrations of elements in the sperm plasma and serum was set for Sr - 252.9 times, Hg - 85.1 times and Al - 20.7 times. The minimum difference between the average concentrations in the sperm plasma and serum was set for B - 0.3 times, Li - 1.1 times and As - 1.8 times. For the remaining elements, the difference between the averages was in the range 2.7 - 8.5 times. It can be assumed that a greater amount of toxic and essential elements accumulate in the accessory gonads and enter the sperm during ejaculation. Authors acknowledge financial support from Russian Science Foundation, Grant No: 17-16-01109, development program of Bioresource collections “Cryobank of genetic recourses the All-Russian Research Institute for Horse Breeding.”
PSI-B-39 Late-Breaking: Animal science career outcomes: influence of gender on research funding from the National Institute of Food and Agriculture
Authors: Goor Angelica Van , Moran Emma , Chitnis Parag , Dean Wesley , Haidet Megan , Kirk-Baer Charlotte , Shoemaker Robbin 
Published: December 2019

Abstract
Background: Overall, women and men in STEM degrees graduate at parity. However, women are not equally represented in academic positions, and this is true for agricultural fields including the animal sciences. The National Institute of Food and Agriculture (NIFA) is the USDA’s primary extramural science agency that administers funding for programs to advance agriculture-related sciences. The Agriculture and Food Research Initiative (AFRI) is NIFA’s flagship granting program that funds research, education, and extension efforts. Our objective was to determine the effect of gender on submission rates and success rates in animal-related AFRI program area priorities. Methods: All applications submitted to AFRI programs in animal genetics, health/welfare, production, and reproduction between 2013–2018 were evaluated. Gender was assigned to Project Directors using Gender API software. Then the effect of gender on submission rates and success rates across and within program areas was determined. Results: A total of 2,018 applications were assigned a gender and examined further. Overall, women submitted 28% (i.e. men submitted 72%) of applications with some variation in different areas of animal sciences (30% in animal health to 24% in animal reproduction). On average, women’s applications had a 24% success rate, compared to men at 21%; women applicants to animal genomics had the highest success rate of 42% while the lowest was in animal health at 19%. Discussion: Women submit fewer proposals than men, consistent with the fact that women hold fewer academic positions needed to apply for research grants such as those from AFRI. Interestingly, women tend to have higher success rates than men. One hypothesis is that the women that hold these academic positions and apply for these research grants are outstanding performers. However, more research is needed to address several important factors that may influence these results such as co-investigators, potential panel biases, and career stage.
PSIV-B-38 Late-Breaking: Effect of Glutamate (Glu) on Developmental Block and the MZT Relative Genes Expression in Mouse embryo during in Vitro Culture
Authors: Liu Yu , Lou An Gang , Yang Shuo , Li Zhong Shu , Fang Nan-Zhu 
Published: December 2019

Abstract
The risk of developmental block in mammal’s embryos is high during in vitro as compare to in vivo environment because the in vitro embryo-culture systems are suboptimal. During in vitro-culture the balance between ROS production and elimination is disturbed and may lead to 2-cell block in mouse embryos [1]. In the current study, we investigated the effects of Glu as anti-developmental block during IVC on ZGA and MZT on mouse embryos. The mouse embryos were divided into control and different level of Glu treated group. The cleavage rate was determined, the ROS and GSH level was investigated using DCHF-DA and CMF2HC respectively. The mRNA expression level of ZGA marker gene such as Eif-1α, Muerv l, Zscan4d and Hsp70.1 was analyzed among the groups using RT-PCR. The transition rate from 2-cell to 4-cell was significantly higher in 6mmol/L Glu treated group as compare to control and others treated groups. No significant difference was recorded in the level of ROS and GSH during MZT stage among the different groups. The mRNA expression level of ZGA marker gene was significantly increased at middle and late stage in 6mmol/L Glu treated group as compare to control and others treated groups. In conclusion, this study shows that the concentration of 6mmol/L Glu could maintain the dynamic balance of GSH and ROS, increase the expression of ZGA marker gene and maintain its high expression pattern of time series, directly participate in the ZGA activated process; ultimately reduce the risk of developmental block to ensure the successful completion of MZT. Reference [1] Lee MT, Bonneau AR, Giraldez AJ.Zygotic Genome Activation during the Maternal-to-Zygotic Transition. Annual Rev Cell Dev Biol [J], 2014, 30:581–613.
PSIX-24 Effects of rice feeding on growth performance and protein (amino acids) metabolism in weaning piglets
Authors: Takada Ryozo , Tasaka Yuusuke 
Published: December 2019

Abstract
This experiment was conducted to clarify the effects of feeding rice on growth performance and protein (amino acids) metabolism in weaning piglets. Sixteen weaning piglets with an average initial weight of 7.5 kg were divided into two groups. One group was fed a corn-soybean meal based diet, and the other was fed a rice-soybean meal diet. Each diet contained about 46% of corn or rice. A 2-week growth trial was conducted and growth performance, dry matter and crude protein digestibility, liver lysine-ketoglutarate reductase activity, plasma urea nitrogen, glucose, insulin, and free amino acids concentrations were measured. The average daily gain (g/d) and feed efficiency (gain/feed) in rice-fed piglets were significantly higher than those in corn-fed piglets. Average feed intake, however, was not significantly different between corn- and rice-fed piglets. Dry matter and and crude protein digestibility were not significantly different between two groups. Liver lysine-ketoglutarate reductase activity tended to be lower (P = 0.073) in rice-fed piglets than that in corn-fed piglets. Plasma urea nitrogen concentration in rice-fed piglets was significantly lower than that in corn-fed piglets. Plasma glucose and insulin concentrations were significantly higher in rice-fed piglets than those in corn-fed piglets. Plasma free valine, isoleucine, tryptophan, and glysine concentrations were significantly higher in rice-fed piglets than those in corn-fed piglets. On the other hand, plasma histidine concentration in rice-fed piglets was significantly lower than that in corn-fed piglets. In conclusion, these results clearly show that rice feeding affects the protein and amino acids metabolism and improves the growth performance in weaning piglets. Rice could be considered to be a good feedstuff for weaning piglets.
PSIX-25 Porcine epidemic diarrhea virus infection impairs intestinal nutrient transport in piglets
Authors: Hou Yongqing , Zhang Junmei , Yi Dan , Wu Tao 
Published: December 2019

Abstract
Porcine epidemic diarrhea virus (PEDV) infection can occur in all ages of pigs, but neonatal piglets are the most susceptible and sensitive to the virus. PEDV infection can cause intestinal dysfunction, severe diarrhea and even death in piglets. This study determined the effects of PEDV infection on the absorptive function and gene expression of nutrient transporters in the small intestine of piglets by microarray assay. Sixteen 7-day-old healthy piglets fed with milk replacer and were randomly allocated to one of two treatments (the Control and PEDV groups). After a 5-day adaptation period, piglets (n = 8) were orally administrated with either sterile saline or PEDV (Yunnan province strain) solution at 104.5TCID50 (50% tissue culture infectious dose) per piglet. On day 5 of the trial, D-xylose (0.1 g/kg BW) was orally administrated to all piglets. One hour later, jugular vein blood samples were collected, and then all piglets were killed to obtain the intestinal samples. Compared with the control, PEDV infection increased (P &lt; 0.05) incidence of diarrhea, plasma DAO activity and iFABP level, while decreased (P &lt; 0.05) plasma D-xylose concentration of piglets. Moreover, PEDV infection altered the amino acids profiles (P &lt; 0.05), and decreased (P &lt; 0.05) the gene expression of AQPs (AQP4, AQP8 and AQP10), amino acids transporters (y+LAT1, b0’+AT, and PepT1), molecules associated with lipid transport and metabolism (LPL, SLC27A2, and ACSL3), and glucose transport and metabolism (GLUT2 and INSR). However, PEDV infection enhanced (P &lt; 0.05) the gene epression of PCK1, ASS1, SGLT1, and CFTR in the jejunum of piglets. Collectively, these comprehensive results indicate that PEDV infection impairs intestinal absorptive function and inhibits the expression of genes associated with nutrient transport and metabolism in piglets.
PSVII-24 Growth performance and organ weight of weaned pigs fed diets with different fiber and fat sources
Authors: Hong Jinsu , Woyengo Tofuko A 
Published: December 2019

Abstract
The objective of this study was to determine the interactive effects of dietary fiber source and lipid source on growth performance and visceral organ weights of weaned pigs. Two hundred and eighty 21 days old pigs [initial body weight (BW) = 6.84 kg] were housed in 40 pens (7 pigs/pen). The pigs were fed 4 diets (10 pens/diet) in a randomized complete block design in 2 phases, Phase 1 from day 0 to 14 and Phase 2 from day 14 to 35. The diets were corn-soybean meal-based with either sugar beet pulp (SBP) or soybean hulls (SBH) as fiber source and either soybean oil (SBO) or choice white grease (CWG) as fat source in 2 × 2 factorial arrangement. The BW and feed intake were determined by phase, whereas visceral organ weights were determined on day 35. Fiber source and fat source did not interact on growth performance and organ weights relative to BW. Pigs fed CWG-containing diets had lower (P &lt; 0.05) gain to feed ratio than those fed SBO-containing diets by 7.95% during Phase 1 of feeding. Pigs fed SBP-containing diets had lower (P &lt; 0.05) gain to feed ratio than those fed SBH-containing diets by 7.94% during Phase 1 of feeding. However, pigs fed SBP-containing diets had greater (P &lt; 0.05) stomach weight, and tended to have greater (P &lt; 0.1) heart, small intestine and large intestine weights relative to BW than those fed SBO-containing diets. In conclusion, replacement of SBH with SBP in diets for weaned pigs reduced feed efficiency and increased visceral organ weights relative to BW, implying that the replacement of SBH with SBP in diets of weaned pigs increased energy expenditure in visceral organs. Inclusion of SBO (at the expense of CWG) in diets for weaned pigs can improve feed efficiency.
PSXI-11 Comparison of once night vs. traditional twice a day feed-delivering schedule on feedlot performance of back-growing cattle in hot climate
Authors: Barajas Ruben , Ramos-Suarez Alejandro , Cervantes-Pacheco Billy J , Chongo-García Bertha 
Published: December 2019

Abstract
In the hot-weather regions, during the hottest hours of day, cattle experienced major difficulties dissipating excessive heat load to the environment. The main source of heat production of cattle is rumen fermentation process. Changing common feeding schedule from day to night hours could help cattle easily dissipate the heat from digestive process to surrounding and impact on its performance. With the objective of comparing once-per-night vs. traditional twice-a-day feed-delivering schedule on feedlot performance of back-growing cattle in hot climate, 80 bull-calves (244.9 kg BW) were involved in a 56 feedlot experiment, performed during summer of 2018 in Culiacan, Mexico. In a complete block design experiment in groups of five were placed in 16 ground pens (6 x 12 m), and inner each block pens were randomly assigned to two treatments: 1) Feeding twice a day at 0900 and 1530 hours (Control); and 2) Feeding once a day at 2000 hours (Night treatment). In animals fed ad libitum with a growing diet, body weight, dry matter intake, average daily gain, and feed efficiency were measured. Pen (five animals) was consider as the experimental unit (8 by treatment). Results were subject to ANOVA and P &lt; 0.05 fitted to accept statistical difference. Across experiment, maximum and minimum temperature were 44.7 and 23.6 °C, respectively; and maximum and minimum THI were 95.99 and 73.07, respectively. Night feeding schedule increased (P &lt; 0.01) in 5% final weight, 25% daily gain, 7% dry matter intake, and 17% feed efficiency (gain: feed ratio). Results suggest that feeding time has a strong influence on feedlot performance of cattle under hot weather. It is concludes that delivering feed during the night hours becomes as a viable feeding strategy to improve feedlot performance of cattle in hot climates.
PSIX-39 Supplementing an immunomodulatory feed ingredient to improve thermoregulation and performance in finishing beef cattle under heat stress conditions
Authors: Colombo Eduardo , Cooke Reinaldo F , Schubach Kelsey , Rett Bruna , Brandão Alice 
Published: December 2019

Abstract
This experiment compared physiological and productive parameters in finishing cattle managed under heat stress conditions, and supplemented or not with Omnigen-AF (Phibro Animal Health, Teaneck, NJ). Crossbred cattle (Bos taurus × B. indicus; 64 heifers and 64 steers) were ranked by initial body weight (BW; 440 ± 3 kg) and sex, and allocated to 1 of 16 pens (8 heifers or steers/pen). Pens within sex were randomly assigned to receive (OMN; n = 8) or not (CON; n = 8) Omnigen-AF at 56 g/cow daily (as-fed basis). Cattle received a total-mixed ration during the experimental period (d 0 to 107), and Omnigen-AF was offered daily as a top-dress to OMN. Cattle were shipped on d 107 to a commercial packing facility for slaughter. Cattle BW was recorded and samples of blood and tail-switch hair collected on d 0, 28, 56, 84, and 106. Feed intake was evaluated biweekly from each pen, and feed efficiency calculated according to total feed intake and BW gain of each pen. From d 0 to 7, 28 to 42, and 84 to 98, heifer intravaginal temperature was recorded hourly (iButton temperature loggers DS1922L, Maxim Integrated, San Jose, CA). Environmental temperature humidity index (THI) was also recorded hourly throughout the experiment, and averaged 79.8 ± 0.6. Heifers receiving OMN had less (P &lt; 0.05) vaginal temperature from 1500 to 1900h (39.05 vs. 39.19 °C), when THI ranged from 85.3 to 90.1. No treatment effects were detected (P &gt; 0.20) for BW gain, feed intake, feed efficiency, and carcass traits. No treatment effects were also detected (P &gt; 0.17) for plasma concentrations of cortisol and haptoglobin, and cortisol concentrations in tail-switch hair. Hence, OMN ameliorated hyperthermia in finishing cattle exposed to heat stress conditions, but such benefit was not sufficient to improve productive and physiological responses.
PSXII-39 Probiotic on finishing phase of Nellore bulls fed tropical grass on dry season
Authors: Calaça Alana M , Silva Mauricia B , Fernandes Juliano , Figueiredo Cibelle B , Teles Nathalia M , Berti Guilherme F , Couto Victor R 
Published: December 2019

Abstract
The objective was to evaluate the effects of Bacillus toyonensis on performance, carcass traits and its influence on ruminal and intestinal mucosa development of Nellore bulls fed tropical grass pasture on dry season supplemented with concentrate at 2.0% BW for 133 d. Four animals were slaughtered at the beginning of the experiment so that the initial carcass yield could be estimated. Eighty Nellore bulls (initial BW 354.02 ± 3.38 kg) were blocked according to body weight and assigned to one of two treatments: concentrate with Bacillus toyonensis (animals fed 1.6 x 109 UFC/day) or without (control group). Then, the remaining animals were allocated in 16 paddocks of Urochloa brizantha with 1.0 ha each (eight paddocks/treatment and five animals/paddock). At the end of the trial, bulls were slaughtered and carcass traits such as HCW, dressing percentage, 12th-rib fat thickness, carcass gain and carcass ADG were obtained. Tissue from the ventral sac and jejunum were collected and fixed in buffered formalin for evaluation of rumen papillae and intestine villi morphology. Data were analyzed using the GLM procedure of SAS. Paddock was the experimental unit for all analyses. Results indicated there were no significant difference (P &gt; 0.05) between treatments for final BW (528.2 kg), ADG (1.333 kg) and G:F (0.165). No significant differences were detected between treatments for HCW (299.8 kg), dressing percentage (56.82%), and carcass ADG (0.885 kg). Conversely, control group showed a significant (P &lt; 0.025) higher 12th-rib fat thickness (4.06 vs 2.97 mm). No significant effect on the development of the ruminal and intestinal epithelium was observed with the use of probiotic. Probiotic based on Bacillus toyonensis did not improve growth performance and carcass characteristics on finishing Nellore bulls fed tropical grass pasture on dry season.