bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comparative Study of Endoscopic versus Microscopic Cartilage Type I Tympanoplasty
Authors: Shakya Dipesh , KC Arun , Nepal Ajit 
Published: November 2019

Abstract
Introduction The use of endoscope is rapidly increasing in otological and neuro-otological surgery in the last 2 decades. Middle ear surgeries, including tympanoplasty, have increasingly utilized endoscopes as an adjunct to or as a replacement for the operative microscope. Superior visualization and transcanal access to diseases normally managed with a transmastoid approach are touted as advantages with the endoscope.
Objectives The present study aimed to compare the outcomes of endoscopic and microscopic cartilage tympanoplasty (Type I)
Methods This was a retrospective comparative study of 70 patients (25 males and 45 females) who underwent type I tympanoplasty between March 2015 and April 2016. The subjects were classified into 2 groups: endoscopic tympanoplasty (ET, n = 35), and microscopic tympanoplasty (MT, n = 35). Tragal cartilage was used as a graft and technique used was cartilage shield tympanoplasty in both groups. Demographic data, perforation size of the tympanic membrane at the preoperative state, operation time, hearing outcome, and graft success rate were evaluated.
Results The epidemiological profiles, the preoperative hearing status, and the perforation size were similar in both groups. The mean operation time of the MT group (52.63 ± 8.68 minutes) was longer than that of the ET group (48.20 ± 10.37 minutes), but the difference was not statistically significant. The graft success rates 12 weeks postoperatively were 91.42% both in the ET and MT groups, that is, 32/35; and these values were not statistically significantly different. There was a statistically significant improvement in hearing within the groups, both pre- and postoperatively, but there was no difference between the groups.
Conclusion Endoscopic tympanoplasty is a minimally invasive surgery with similar graft success rate, comparable hearing outcomes and shorter operative time period as compared to microscopic use.
Pure-Tone Hearing Thresholds and Brainstem Auditory Evoked Potentials in Sporadic Ataxia
Authors: Zeigelboim Bianca Simone , Scheidt Anylize Wachholz Vom , Kronbauer Kairone Fernandes , Liberalesso Paulo Breno Noronha , José Maria Renata , Fonseca Vinicius Ribas , Teive Hélio Afonso Ghizoni 
Published: November 2019

Abstract
Introduction Spinocerebellar ataxia (SCA) is part of a genetic and clinical heterogeneous group of neurodegenerative diseases characterized by progressive cerebellar ataxia.
Objective To describe the results of audiological and electrophysiological hearing evaluations in patients with sporadic ataxia (SA).
Methods A retrospective cross-sectional study was carried out with 11 patients submitted to the following procedures: anamnesis, otorhinolaryngological evaluation, tonal and vocal audiometry, acoustic immittance and brainstem auditory evoked potential (BAEP) tests.
Results The patients presented with a prevalence of gait imbalance, of dysarthria, and of dysphagia; in the audiometric and BAEPs, four patients presented with alterations; in the acoustic immittance test, five patients presented with alterations, predominantly bilateral.
Conclusion The most evident alterations in the audiological evaluation were the prevalence of the descending audiometric configuration between the frequencies of 2 and 4 kHz and the absence of the acoustic reflex between the frequencies of 3 and 4 kHz bilaterally. In the electrophysiological evaluation, the patients presented changes with a prevalence of increased I, III and V wave latencies and the interval in the interpeak I-III, I-V and III-V. In the present study, it was observed that auditory complaints did not have a significant prevalence in this type of ataxia, which does not occur in some types of autosomal recessive and dominant ataxia.
Behavioral and Electrophysiological Assessment of Adults Who Underwent Cochlear Implantation After Hearing Aid Experience
Authors: Calderaro Victor Goiris , Amaral Maria Stella Arantes do , Luz Benedita Aparecida Borges da , Bernal Sarah Carolina , Hyppolito Miguel Ângelo , Reis Ana Claudia Mirândola Barbosa 
Published: November 2019

Abstract
Introduction Cochlear implantation has been considered a viable option to restore hearing perception in adults with severe to profound postlingual hearing loss.
Objectives To analyze behavioral hearing responses and P300 latency and amplitude measurements in adults with bilateral sensorineural hearing loss at two phases, first when they were using hearing aids (HAs) and, then, after 12 months of cochlear implant (CI) use. The association between behavioral and electrophysiological evaluations was explored, as it is believed that the study of auditory processing with different hearing devices can contribute to future CI adjustments and fittings, especially for patients who cannot give subjective feedback (such as small children and individuals with multiple disabilities).
Methods Prospective comparative study (Ethical approval 11489/2014). Twelve adults were assessed, 7 males and 5 females, in the 22 to 76 years old age range, who had undergone CI surgery after HA experience.
Results The analyses showed an improvement of hearing thresholds when patients started using CIs. Comparing data from P300 latency measurements, there was an increase of the P300 wave post-CI at Cz and Fz. Regarding the amplitude, P300 mean values decreased at Cz, but increased at Fz. There was no significant correlation between behavioral and electrophysiological assessment and the variables age, gender, auditory deprivation, and electronic device used.
Conclusion There was a significant improvement of hearing thresholds after twelve months of CI experience. The mean latency values of P300 after 12 months of CI use increased at Cz and Fz, while mean amplitude values decreased at Cz and increased at Fz.
Mechanotransduction in Liver Diseases
Authors: Kang Ningling 
Published: November 2019

AbstractChronic liver diseases, such as fibrosis and cancer, lead to a rigid or stiff liver because of perpetual activation of hepatic stellate cells or portal fibroblasts into matrix-producing myofibroblasts. Mechanical forces, as determined by the mechanical properties of extracellular matrix or pressure of circulating blood flow/shear stress, are sensed by mechanoreceptors at the plasma membrane and transmitted into a cell to impact cell function. This process is termed as mechanotransduction. This review includes basic knowledge regarding how external forces are sensed, amplified, and transmitted into the interior of a cell as far as the nucleus to regulate gene transcription and generate biological responses. It also reviews literatures to highlight the mechanisms by which mechanical forces in a normal or diseased liver influence the phenotype of hepatocytes, hepatic stellate cells, portal fibroblasts, and sinusoidal endothelial cells, and these cells in turn participate in the initiation and progression of liver diseases.
Projections of care for older people with dementia in England: 2015 to 2040
Authors: Wittenberg Raphael , Hu Bo , Jagger Carol , Kingston Andrew , Knapp Martin , Comas-Herrera Adelina , King Derek , Rehill Amritpal , Banerjee Sube 
Published: November 2019

Abstract

Background
The number of older people with dementia and the cost of caring for them, already substantial, are expected to rise due to population ageing.

Objective
This study makes projections of the number of older people with dementia receiving unpaid care or using care services and associated costs in England.

Methods
The study drew on up-to-date information for England from multiple sources including data from the CFASII study, output from the PACSim dynamic microsimulation model, Office for National Statistics population projections and data from the MODEM cohort study. A simulation model was built to make the projections.

Results
We project that the number of older people with dementia will more than double in the next 25 years. The number receiving unpaid or formal care is projected to rise by 124%, from 530,000 in 2015 to 1,183,000 in 2040. Total cost of dementia is projected to increase from £23.0 billion in 2015 to £80.1 billion in 2040, and average cost is projected to increase from £35,100 per person per year in 2015 to £58,900 per person per year in 2040. Total and average costs of social care are projected to increase much faster than those of healthcare and unpaid care.

Conclusion
The numbers of people with dementia and associated costs of care will rise substantially in the coming decades, unless new treatments enable the progression of the condition to be prevented or slowed. Care and support for people with dementia and their family carers will need to be increased.
The evolution of contact prediction: evidence that contact selection in statistical contact prediction is changing
Authors: Chonofsky Mark , de Oliveira Saulo H P , Krawczyk Konrad , Deane Charlotte M 
Published: November 2019

Abstract

Motivation
Over the last few years, the field of protein structure prediction has been transformed by increasingly accurate contact prediction software. These methods are based on the detection of coevolutionary relationships between residues from multiple sequence alignments (MSAs). However, despite speculation, there is little evidence of a link between contact prediction and the physico-chemical interactions which drive amino-acid coevolution. Furthermore, existing protocols predict only a fraction of all protein contacts and it is not clear why some contacts are favoured over others. Using a dataset of 863 protein domains, we assessed the physico-chemical interactions of contacts predicted by CCMpred, MetaPSICOV and DNCON2, as examples of direct coupling analysis, meta-prediction and deep learning.

Results
We considered correctly predicted contacts and compared their properties against the protein contacts that were not predicted. Predicted contacts tend to form more bonds than non-predicted contacts, which suggests these contacts may be more important than contacts that were not predicted. Comparing the contacts predicted by each method, we found that metaPSICOV and DNCON2 favour accuracy, whereas CCMPred detects contacts with more bonds. This suggests that the push for higher accuracy may lead to a loss of physico-chemically important contacts. These results underscore the connection between protein physico-chemistry and the coevolutionary couplings that can be derived from MSAs. This relationship is likely to be relevant to protein structure prediction and functional analysis of protein structure and may be key to understanding their utility for different problems in structural biology.

Availability and implementation
We use publicly available databases. Our code is available for download at https://opig.stats.ox.ac.uk/.

Supplementary information
Supplementary information is available at Bioinformatics online.
Comparison of neutral and adaptive differentiation in the Mediterranean grass Brachypodium retusum
Authors: Vidaller Christel , Baumel Alex , Juin Marianick , Dutoit Thierry , Bischoff Armin 
Published: November 2019

Abstract
The Mediterranean grass Brachypodium retusum is the dominant species of a vulnerable steppe habitat. Differentiation in phenotypic traits has been found in a previous study, but scales and drivers are largely unknown. In this study, we compared molecular and phenotypic variation between populations to (1) analyse spatial patterns of neutral genetic variation; (2) test for effects of selection on differentiation and (3) identify major drivers of adaptive differentiation. We collected plant material of 17 populations in the western Mediterranean covering a large part of the species range. Neutral population differentiation was estimated using AFLP markers. A regional-scale subset of pairs of French populations was sampled in close proximity from calcareous and red Mediterranean soils as major habitat types. Sampling sites differed in climate at a regional scale and in soil characteristics such as pH at a local scale. These populations were grown in a common garden experiment to measure phenotypic traits. To test for the effects of selection on phenotypic differentiation, we calculated pairwise θST values based on neutral AFLP markers and compared them to pairwise PST values using phenotypic traits. Global dataset θST indicated significant neutral genetic differentiation between western Mediterranean populations. In the French populations, PST of vegetative and reproductive traits were higher than θST, suggesting that directional selection contributed to phenotypic population differentiation. We also found significant local-scale differentiation between soil types, but differentiation was substantially higher at the regional scale, pointing towards climate as a stronger selective factor than soil type. Mean temperature of the hottest month and winter frost frequency were identified as major drivers of adaptive differentiation. The study demonstrated the importance of combining neutral marker and phenotypic trait analysis at different spatial scales to evaluate genetic structure. Despite relatively low differentiation in AFLP markers, environmental pressure was sufficient to maintain phenotypic differentiation at regional scales.
Genome size and chromosome number conservation contrasting with karyotype diversity in Hohenbergia (Bromelioideae, Bromeliaceae)
Authors: Gonçalves-Oliveira Rodrigo Cesar , Ximenes Amanda Fagundes , Oliveira Ana Rafaela , Vasconcelos Santelmo , Carvalho-Filho Nelson , Oliveira Guilherme , de Campos José Marcello Salabert , Brasileiro-Vidal Ana Christina , Facio Viccini Lyderson , Benko-Iseppon Ana Maria 
Published: November 2019

AbstractPlant evolution may be triggered by significant chromosome changes. In some plant groups, karyoevolution played an important role, influencing speciation processes. Hohenbergia comprises 48 species distributed through eastern Brazil. Previous cytological information includes few species and only chromosome counts, lacking information about genome size and more accurate karyomorphological investigation. Here, we compare cytomolecular features and genome sizes of 12 Hohenbergia spp. Besides, new measurements of genome sizes of 32 species are reported. All studied species presented 2n = 50, a number prevalent in Bromelioideae. The genome sizes (2C) varied from 0.74 to 1.52 pg. Despite the apparent homogeneity in chromosome number and genome size in Hohenbergia, significant polymorphism was observed in regard to the distribution of CMA+/DAPI0 bands and sites of 35S and 5S rDNA in metaphase chromosomes. Seven out of 12 analysed species presented heteromorphic pairs regarding 35S rDNA and/or 5S rDNA. Hohenbergia thus shows karyotypic diversity despite the conservation in chromosome number.
China’s Rise and Balance of Power Politics
Authors: Han Zhen , Paul T V 
Published: November 2019

Abstract
The post-Cold War international system, dominated by the United States, has been shaken by the relative downturn of the US economy and the simultaneous rise of China. China is rapidly emerging as a serious contender for America’s dominance of the Indo-Pacific. What is noticeable is the absence of intense balance of power politics in the form of formal military alliances among the states in the region, unlike state behaviour during the Cold War era. Countries are still hedging as their strategic responses towards each other evolve. We argue that the key factor explaining the absence of intense hard balancing is the dearth of existential threat that either China or its potential adversaries feel up till now. The presence of two related critical factors largely precludes existential threats, and thus hard balancing military coalitions formed by or against China. The first is the deepened economic interdependence China has built with the potential balancers, in particular, the United States, Japan, and India, in the globalisation era. The second is the grand strategy of China, in particular, the peaceful rise/development, and infrastructure-oriented Belt and Road Initiative. Any radical changes in these two conditions leading to existential threats by the key states could propel the emergence of hard-balancing coalitions.
Transient receptor potential vanilloid-4 contributes to stretch-induced hypercontractility and time-dependent dysfunction in the aged heart
Authors: Veteto Adam B , Peana Deborah , Lambert Michelle D , McDonald Kerry S , Domeier Timothy L 
Published: November 2019

Abstract

Aims
Cardiovascular disease remains the greatest cause of mortality in Americans over 65. The stretch-activated transient receptor potential vanilloid-4 (TRPV4) ion channel is expressed in cardiomyocytes of the aged heart. This investigation tests the hypothesis that TRPV4 alters Ca2+ handling and cardiac function in response to increased ventricular preload and cardiomyocyte stretch.

Methods and results
Left ventricular maximal pressure (PMax) was monitored in isolated working hearts of Aged (24–27 months) mice following preload elevation from 5 to 20mmHg, with and without TRPV4 antagonist HC067047 (HC, 1 µmol/L). In preload responsive hearts, PMax prior to and immediately following preload elevation (i.e. Frank–Starling response) was similar between Aged and Aged+HC. Within 1 min following preload elevation, Aged hearts demonstrated secondary PMax augmentation (Aged&gt;Aged+HC) suggesting a role for stretch-activated TRPV4 in cardiac hypercontractility. However, after 20 min at 20 mmHg Aged exhibited depressed PMax (Aged&lt;Aged+HC) suggestive of TRPV4-dependent contractile dysfunction with sustained stretch. To examine stretch-induced Ca2+ homeostasis at the single-cell level, isolated cardiomyocytes were stretched 10–15% of slack length while measuring intracellular Ca2+ with fura-2. Uniaxial longitudinal stretch increased intracellular Ca2+ levels and triggered Ca2+ overload and terminal cellular contracture in Aged, but not Aged+HC. Preload elevation in hearts of young/middle-age (3–12 months) mice produced an initial PMax increase (Frank–Starling response) without secondary PMax augmentation, and cardiomyocyte stretch did not affect intracellular Ca2+ levels. Hearts of transgenic mice with cardiac-specific TRPV4 expression exhibited PMax similar to 3- to 12-month control mice prior to and immediately following preload elevation but displayed secondary PMax augmentation. Cardiomyocytes of mice with transgenic TRPV4 expression were highly sensitive to mechanical stimulation and exhibited elevated Ca2+ levels, Ca2+ overload, and terminal contracture upon cellular attachment and stretch.

Conclusion
TRPV4 contributes to a stretch-induced increase in cardiomyocyte Ca2+ and cardiac hypercontractility, yet sustained stretch leads to cardiomyocyte Ca2+ overload and contractile dysfunction in the aged heart.
The Bee Community of Cannabis sativa and Corresponding Effects of Landscape Composition
Authors: Flicker Nathaniel Ryan , Poveda Katja , Grab Heather 
Published: November 2019

Abstract
Industrial hemp, Cannabis sativa (Cannabaceae), is a newly introduced and rapidly expanding crop in the American agricultural landscape. As an exclusively wind-pollinated crop, hemp lacks nectar but produces an abundance of pollen during a period of floral dearth in agricultural landscapes. These pollen resources are attractive to a range of bee species but the diversity of floral visitors and their use of hemp across a range of agricultural contexts remains unclear. We made repeated sweep net collections of bees visiting hemp flowers on farms in New York, which varied in both landscape context and phenotypic traits of hemp varieties. We identified all bee visitors to the species level and found that hemp supported 16 different bee species. Landscape simplification negatively impacted the abundance of bees visiting hemp flowers but did not affect the species richness of the community. Plant height, on the other hand, was strongly correlated with bee species richness and abundance for hemp plots with taller varieties attracting a broader diversity of bee species. Because of its temporally unique flowering phenology, hemp has the potential to provide a critical nutritional resource to a diverse community of bees during a period of floral scarcity and thereby may help to sustain agroecosystem-wide pollination services for other crops in the landscape. As cultivation of hemp increases, growers, land managers, and policy makers should consider its value in supporting bee communities and take its attractiveness to bees into account when developing pest management strategies.
The effect of population bottleneck size and selective regime on genetic diversity and evolvability in bacteria
Authors: Wein Tanita , Dagan Tal 
Published: November 2019

Abstract
Population bottlenecks leading to a drastic reduction of the population size are common in the evolutionary dynamics of natural populations; their occurrence is known to have implications for genome evolution due to genetic drift, the consequent reduction in genetic diversity and the rate of adaptation. Nevertheless, an empirical characterization of the effect of population bottleneck size on evolutionary dynamics of bacteria is currently lacking. Here we show that selective conditions have a stronger effect on the evolutionary history of bacteria in comparison to population bottlenecks. We evolved Escherichia coli populations under three different population bottleneck sizes (small, medium, large) in two temperature regimes (37 °C and 20 °C). We find a high genetic diversity in the large in comparison to the small bottleneck size. Nonetheless, the cold temperature led to reduced genetic diversity regardless the bottleneck size, hence, the temperature has a stronger effect on the genetic diversity in comparison to the bottleneck size. A comparison of the fitness gain among the evolved populations reveals a similar pattern where the temperature has a significant effect on the fitness. Our study demonstrates that population bottlenecks are an important determinant of bacterial evolvability; their consequences depend on the selective conditions and are best understood via their effect on the standing genetic variation.
Cytomegalovirus antibody responses associated with increased risk of TB disease in Ugandan adults
Authors: Stockdale Lisa , Nash Stephen , Farmer Ruth , Raynes John , Malikaarjun Suresh , Newton Robert , Fletcher Helen A 
Published: November 2019

Abstract

Background
Recent evidence highlights human cytomegalovirus (HCMV) and immune activation as risk factors for tuberculosis (TB) disease. It is not known whether other herpes viruses are also implicated, nor if a dose-response relationship exists between TB risk and herpes co-infection.

Methods
This nested case-control study used stored serum samples from 25 TB cases up to 10 years prior to TB diagnosis and between 3 and 6 matched non-TB controls from a rural Ugandan cohort. Samples were investigated for Epstein Barr (EBV), Herpes Simplex (HSV), and HCMV-specific IgG, serum markers of inflammation, and mycobacterial antibody levels.

Results
Humoral response to HCMV, but not EBV or HSV was associated with increased risk of active TB disease up to 10 years prior to diagnosis. Individuals with medium HCMV IgG were 2.8 times more likely to have TB (p=0.055), and those with high HCMV IgG 3.4 times more likely to have TB (p=0.007). Mycobacterial antibody levels were not associated with differences in odds of TB disease. IP-10 was independently associated with increased odds of TB; OR 4.2, p=0.009.

Conclusions
These data provide evidence of a dose response between magnitude of HCMV IgG with risk of TB disease. An inflammatory environment, characterized by serum IP-10 and IL1α, are independently associated with increased risk of TB disease.