bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tests of Impregnation Speed of Electrotechnical Pressboard with Insulating Oil
Authors: Kozak C. 
Published: June 2020

The paper presents a new test stand for ivestigating the rate of penetration of transformer oil through electrotechnical pressboard. The stand consists of a pipe, to the lower end of which is glued a pressboard plate. The pipe is filled with insulating oil. A mirror is placed under the plate, which directs its image to the lens of the camera, which takes a series of photographs at a given time interval. After being saturated with the insulating oil, the pressboard changes colour from light to dark yellow. The absorbing time is defined as the time in which a dark yellow spot appears on the lower light surface of the pressboard after the pipe is filled with oil.A new way of determining capillary diameters has been developed when the number of capillaries is unknown and the volume of liquid flowing through them is not measurable. The distribution of the times of penetration of transformer oil through 2 mm thick electrotechnical pressboard was determined, the values of which range from about 220 min to about 550 min. It was found that the radii of capillaries through which the insulating oil penetrates are within the range from about 45 nm to about 70 nm. Due to the structure of the pressboard, which consists of cellulose fibres, arranged more or less tightly, there are capillaries in the structure of the board, each of which has sections of varying lengths of radii. This means that short sections of a single capillary can have radii both smaller than 45 nm and larger than 70 nm.The developed stand and the new analysis method can be used for testing various porous materials for penetration by various liquids.
H.Pylori Infection Induces CXCL8 Expression and Promotes Gastric Cancer Progress Through Downregulating KLF4
Authors: liu zhengxia , Wu Xiao , Tian Yuanyuan , zhang wanchun , qiao siyuan , xu wenting , liu yakun , Wang Siying 
Published: June 2020

Abstract
The authors have withdrawn this preprint due to erroneous posting.
Lower expression of the RASSF10 gene induces myeloma cell proliferation due to hypermethylation of the gene promoter in multiple myeloma
Authors: Chen Jin , Liu Hui , Liu Zhaoyun , Shao Qing , Jiang Fengjuan , Yan Siyang , Li Lijuan , Song Jia , Ding Kai , Fu Rong 
Published: June 2020

Abstract
Background: Multiple myeloma (MM) is an incurable malignant neoplasm of plasma cells, in which genetic defects, epigenetic aberrations and bone marrow microenvironment are involved in the pathogenesis. RASSF10 acts as a tumor suppressor gene by methylation in glioma and several other cancers, but its role in MM remains unknown. Methods: In order to explore the role of RASSF10, mRNA expression was detected in MM patients and analyzed with overall survival. Results: Expression of the RASSF10 gene significantly decreased in newly diagnosed MM patients, and was positively correlated with overall survival. RPMI-8226 and OPM-2 cell lines with lower RASSF10 expression were selected for further study. Overexpression of RASSF10 in these two cell lines inhibited proliferation and induced apoptosis. The RASSF10 gene promoter in MM cell lines was hypermethylated, and downregulated after decitabine treatment. Meanwhile, expression of the RASSF10 gene was upregulated. MM cells with overexpression of RASSF10 were injected into nude mice and exerted anti-MM activity in vivo. Conclusions: Low expression of RASSF10 contributed to the proliferation of myeloma cells by hypermethylation of its promoter.
Co-loading of Levodopa and Curcumin Using Brain-targeted Protocells as a Drug Delivery System for Improving the Efficacy of Parkinson's Disease
Authors: zhou wenkai , Liu Chang , Yu Feifei , Niu Xia , Wang Xiaomei , Li Guiling , Li Xinru 
Published: June 2020

Abstract
Parkinson's disease (PD), one of the most common movement and neurodegenerative disorders, is challenging to treat, partly because the blood-brain barrier blocks passage of most drugs. Levodopa is a common clinical drug for controlling the symptoms of PD, but it only replenishes the missing dopamine, can’t protect dopaminergic neurons. While curcumin as a neuroprotective agent has been reported for treatment of PD. Herein, we present a novel organic-inorganic composite nanoparticle with brain targeting (denoted as lf-protocells) for co-delivery of levodopa and curcumin, and demonstrate its attractive use as a biocompatible platform for PD treatment. The nanoparticle system is comprised of a lactoferrin (lf) modified lipid bilayer (LB) containing curcumin as its outer membrane and mesoporous silica nanoparticles (MSNs) containing levodopa as its supporting inner core. Our studies illustrate that the lf-protocells have a size of about 180 nm and spherical morphology, and can be used to co-load levodopa and curcumin efficiently. Further, a cell model and a mouse model induced by rotenone (Rot) and MPTP respectively are used to investigate the effects of binary-drug loaded lf-protocells on PD. Our results demonstrate that the combination of curcumin and levodopa alleviate the apoptosis of PD cells, enhance the cell viability as compared to levodopa used alone; levodopa together with curcumin also efficiently decrease the expression of a-synuclein, increase the expression of tyrosine hydroxylase in SH-SY5Y cells, and transform more levodopa into dopamine for supplement the loss of the brain. Moreover, the resulting binary-drug loaded lf-protocells ameliorate oxidative stress and mitochondrial dysfunction as compared to combination of free drugs. In addition, testing in a mouse model indicate that lf-protocells can improve significantly the motor function and distribution in brain compared with unmodified protocells. In conclusion, binary-drug loaded lf-protocells show much better therapeutic efficacy in both the cell model and the mouse model of PD than single free drug and lower toxicity than bare MSNs. These results suggest that lf-protocells can be used as a promising drug delivery platform for targeted therapy against PD and other diseases of the central nervous system.
Optimization The Effect of Roasting Conditions by Central Composite Design (CCD) Method on The Antioxidant Compounds of Opuntia Ficus Indica Seeds From Morocco
Authors: Guezzane Chakir El , Moudden Hamza El , Harhar Hicham , Zarrouk Abdelkader , Tabyaoui Mohamed 
Published: June 2020

Abstract
The effect of roasting conditions on antioxidant activity Opuntia Ficus Indica seeds from Morocco and their phenolic compounds were optimized by the Central Composite Design (CDD) method. The CCD was used to optimize the roasting conditions: temperature (X1: 60–200 °C) and roasting time (X2: 10–50 min). The best roasting conditions were used in order to optimize the optimal value of the response : TPC (Total Phenolic Content): X1: 200°C, X2: 50min with 104.86±1.94GAE/g extract predicted response, TFC (Total Flavonoids Content) : X1: 200 °C, X2: 50 min with 81.23±0.90 mg QE/g extract predicted responses, TTC (Total Condensed Tannins Content): X1: 128.9°C, X2: 34.92min with 6.12±0.95 mg QAE/g extract) predicted response. Moreover, the optimal potential antioxidant activity by ABTS assay and DPPH assay was found in extracts of Opuntia Ficus Indica seeds roasted: at a high temperature of the roasting. Furthermore, the positive significant correlations were determined by the principal component analysis (PCA) on the one hand, between the antioxidant capacity of the extracts and their antioxidants compounds (TPC and TFC), and on the other hand, between the two assays ABTS and DPPH. Consequently, the results of this work indicated that roasted Opuntia Ficus Indica seeds from Morocco can be considered as an essential ingredient for human foods.
The Effect of Endo-1,4-β-Xylanase From Thermomyces Dupontii KKU−CLD−E2−3 on Eucalyptus Pulp Bleaching and Effluent Treatment
Authors: Seemakram Wasan , Harada Hiroyuki , Ekprasert Jindarat , Boonlue Sophon 
Published: June 2020

Abstract
Endo-1,4-β-xylanase-chlorine dioxide bleaching of eucalyptus pulp and analysis of effluent was investigated. The eucalyptus pulp bleaching in D0 stage was prepared with enzyme dose 100 Unit of 10% consistency and incubated at 70 °C for 30 min. The brightness level was achieved up to 72.50 (% ISO). The kappa number and viscosity of eucalyptus pulp was found to be 1.70 and 8.90 (cp), respectively. The paper from pulp bleaching with enzyme has shown an increase in brightness, breaking length, bursting strength, and tearing resistance to be 89.60% IOS, 37.27 Nm/g, 138.81 kPa and 218.02 mN, respectively. The SEM and FTIR analysis of pulp fibers revealed a significance of morphological and structural changes. The analysis of the effluent also showed statistically-significant differences in TOC, BOD, TDS and TSS. The FTIR analysis of the effluent showed the organic compounds and chloride dioxin in cases of those treated by chemicals.
Elevation of serum alpha-1-acid glycoprotein in children with bronchial pneumonia caused by Mycoplasma pneumoniae&nbsp;infection
Authors: Fang Huanying , Zhou Aiping , yang shusheng 
Published: June 2020

Abstract
Background:Alpha-1-acid glycoprotein(AGP) is an acute phase protein which can be elevated under inflammatory conditions. Few studies have explored the use of monitoring serum AGP in Mycoplasma pneumoniae pneumonia(MPP). Methods:We aimed to evaluate the relationship between serum AGP and other convention inflammation bio-markers,in order to explore its clinical significance in children with MPP. Pediatric inpatients with bronchopneumonia from January to December 2018 were enrolled and divided into two groups -children with MPP and children without MPP severed as controls. Serum AGP, procalcitonin (PCT) ,and other laboratory examinations were compared and multiple logistic regressions analysis was used to select the main related factors, then receiver-operating characteristic curves were used to evaluate the value of the selected markers used to identify MPP from bronchopneumonia. Results: Two hundred and sixty-eight children were enrolled in this study.Among them, 87 (32.46%) cases were diagnosed as MPP and 181 gender-matched children were without MPP. Children with MPP were slightly older than those without MPP and had lower levels of serum PCT (P<0.05). In addition, serum AGP was significantly higher in patients with MPP than control participants (136.06±46.37 vs. 122.29±44.21,respectively;P=0.020). Multiple logistic regressions analysis adjusted for age showed that only AGP was the main related factor ,which could perhaps be used to identify MPP from common bronchopneumonia and its area under the curve (AUC) was 0.703(95% CI: 0.634-0.772;P=0.000).Combination with AGP and PCT could slightly improve the AUC(95 % CI) to 0.732 (95% CI: 0.666-0.797, P=0.000). Conclusion: AGP is perhaps a potential bio-marker to distinguish MPP from other bronchopneumonia in children and assist early diagnosis and treatment as soon as possible for it is relatively convenient and fast to be carried out.
INT-777 Improves Cognitive Impairment after Sepsis by Attenuating Neuroinflammation Through the TGR5/cAMP/PKA/CREB Signaling Axis in Rats
Authors: Jin Peng , Deng Shuixiang , Tian Mi , Wei Pengju , Wang Yao , Tan Jiaying , Wen Huimei , Zhao Feng , Gao Yanqin , GONG YE 
Published: June 2020

Abstract
Background: Sepsis survivors are left with significant cognitive and behavioral impairments after discharge, but research on the relevant mechanisms and interventions remains lacking. TGR5 plays a neuroprotective role in many neurologic disease models through different mechanisms. To date, no studies have assessed the effects of TGR5 on neuroinflammation or cognitive or behavioral changes in sepsis models.Methods: A total of 267 eight-week-old male Sprague-Dawley rats were used in this study. Sepsis was induced by cecal ligation and puncture (CLP). All animals received volume resuscitation. The rats were given TGR5 CRISPR oligonucleotide intracerebroventricularly 48 hours before CLP surgery. INT-777 was administered intranasally 1 hour after CLP, and the cAMP inhibitor SQ22536 was administered intracerebroventricularly 1 hour after CLP. Survival rate, bodyweight change, clinical score, neurobehavioral tests, western blot, and immunofluorescence staining were performed. The cognitive function of rats was measured by Morris water maze during 15-20 days after CLP.Results: The expression of TGR5 in the rat hippocampus was upregulated and peaked at 3 days after CLP. The survival rate of rats after CLP was less than 50%, and the growth rate in terms of weight was significantly decreased, while these changes were not improved by INT-777 treatment. However, INT-777 treatment reduced the clinical scores of rats at 24 hours after CLP. On day 15 and later, the surviving mice completed a series of behavioral tests. CLP rats showed spatial and memory deficits and anxiety-like behaviors, and INT-777 treatment significantly improved these effects. Mechanistically, immunofluorescence analysis showed that INT-777 treatment reduced the number of microglia in the hippocampus, neutrophil infiltration and the expression of inflammatory factors after CLP in rats. Moreover, INT-777 treatment significantly increased the expression of TGR5, cAMP, p-PKA, and p-CREB and downregulated the expression of IL-1β, IL-6 and TNF-α. CRISPR-mediated TGR5 knockdown and SQ22536 treatment abolished the neuroprotective effects of TGR5 activation after CLP.Conclusion: This study demonstrates that INT-777 treatment reduced neuroinflammation and microglial cell activation, and then improved cognitive impairment in the experimental sepsis rats. TGR5 has translational potential as a therapeutic target to improve neurological outcomes in sepsis survivors.
Analysis of Changes in Seismicity Pattern for Probabilistic Seismic Hazard Analysis (PSHA) and Mitigation: Mapping Probability Difference before the Large Earthquake and Its Implementation in PSHA &amp; Mitigation around Northern Sumatra
Authors: Triyoso Wahyu , Yudistira Tedi , Sahara David P. 
Published: June 2020

Abstract
The probability difference before the occurrence of a large earthquake is mapped in the northern part of Sumatra, taking the range of Region Time Length (RTL) before a major earthquake (December 26, 2004) around 15 years. By normalizing the absolute value of probability difference between two periods of RTL and before RTL, the Seismic Quiescence Index (SQI) is then defined. Probability difference analysis is done by dividing observations of shallow earthquake periods into two periods based on the similarity gradient of the annual earthquake production, namely 1963-1990 and 1991-November 2004. The results showed that areas with relatively high SQI were consistent with the presence of major earthquake events after November 2004 to 2016 that are sorted by a radius of 300 km with the center point being the epicenter position of the December 2004 earthquake. The implementation of the SQI was then used for probabilistic seismic hazards study and analysis based on an integratedmodel that is derived based on the estimated of seismicity rate of around the subduction zone and active fault of Sumatra Fault Zone (SFZ) sources. The map of Probabilistic Seismic Hazard Analysis (PSHA) is then constructed based onPeak Ground Acceleration (PGA) estimated for a 10% Probability Exceedance (PE) level in 50 years. The results of this study may be very useful for earthquake mitigation and modeling efforts for PSHA going forward.
Landscape of Granulocyte Telomere Shortening in Cytopenia in India
Authors: MANDAL PARAMITA , Dutta Shyamali 
Published: June 2020

Abstract
Objectives: Telomere length and telomerase activity have been implicated in the control of cell proliferation and genomic stability. We recently introduced a novel technique for measurement of the average telomere length in cells using fluorescence in situ hybridization (FISH) with peptide nucleic acid (PNA) probes and flow cytometry (flow-FISH). Results: Using this technique, we have analyzed the telomere length kinetics in subpopulations of unseparated peripheral blood leukocytes (PBLs) in a large population of healthy donors as well as patients with cytopenia. We only found significantly shortened telomeres from patient with cytopenia compared to age-adjusted controls. Strikingly, the telomere length in granulocytes from cytopenia patients differed significantly from controls. Furthermore, an inverse correlation between of granulocyte telomere length and age was found among healthy controls. However, surprisingly, telomere length in cytopenia patients with hypocellular marrow was significantly shorter from patients with non-hypocellular marrow. These results support the concept of accelerated granulocyte telomere shortening in cytopenia patients in India and suggest a potential use of telomere-length measurements as a prognostic tool in this group of disorders as well.
Shifted Phase of EEG Cross-Frequency Coupling in Individuals with Phelan-McDermid Syndrome
Authors: Mariscal Michael G , Berry-Kravis Elizabeth , Buxbaum Joseph D , Ethridge Lauren E , Filip-Dhima Rajna , Foss-Feig Jennifer H , Kolevzon Alexander , Modi Meera E , Mosconi Matthew W , Nelson Charles A , Powell Craig M , Siper Paige M , Soorya Latha , Thaliath Andrew , Thurm Audrey , Zhang Bo , Sahin Mustafa , Levin April R 
Published: June 2020

Abstract
Background Phelan-McDermid Syndrome (PMS) is a rare condition caused by deletion or mutation of the SHANK3 gene. Individuals with PMS frequently present with intellectual disability, symptoms of autism spectrum disorder (ASD), and other neurodevelopmental challenges. Electroencephalography (EEG) can provide a window into network-level function in PMS. Methods Here, we analyze EEG data collected across multiple sites in individuals with PMS (n = 26) and typically developing individuals (n = 15). We quantify oscillatory power, phase-amplitude coupling strength, and phase bias, a measure of the phase of cross frequency coupling thought to reflect the balance of feedforward and feedback activity. Results We find individuals with PMS display increased phase bias (U = 3.841, p < 0.0005), predominantly over posterior electrodes. Most individuals with PMS demonstrate positive overall phase bias while most typically developing individuals demonstrate negative overall phase bias. Among individuals with PMS, strength of phase-amplitude coupling was associated with Sameness, Ritualistic, and Compulsive behaviors as measured by the Repetitive Behavior Scales-Revised (Beta= 0.545, p= 0.011). Conclusions Increased phase bias suggests potential circuit-level mechanisms underlying phenotype in PMS, offering opportunities for back-translation of findings into animal models and targeting in clinical trials.
Effects of regulatory exercise on lipolysis pathway in obese pre and postmenopausal women
Authors: Park Kyu-Min , Lim Seung-Taek , Sung Kun-Young , Kang Sunghwun 
Published: June 2020

Abstract
Background and objectives: The purpose of study was to examine the effects of regulatory resistance exercise for 12 weeks on lipolysis pathway in pre- and post- menopausal women with obesity. Methods: Twenty-three pre- and post- menopausal women with body fat percentages of 30% or more divided into pre- menopausal group (n=9) and post- menopausal group (n=14). All subjects participated in resistance exercise training for 12 weeks. All participant’s anthropometric measurements and physical fitness tests were performed. Protein analyses were performed with subcutaneous fatty tissue extracted, and the samples were analyzed of relevant protein expression changes by using Western blotting. All serum samples were submitted for enzyme-linked immunosorbent assay measurements of adipocyte factors. Results: After 12 weeks between pre- menopausal and post- menopausal groups ATGL, MGL and PLIN protein expression were significantly lower in the post- menopausal group than in the pre- menopausal group. HSL protein expression were significantly higher in the post- menopausal group than in the pre- menopausal group. In addition, leptin concentration was significantly decreased, and adiponectin concentration was significantly increased after resistance exercise in the post- menopausal group more than pre- menopausal group. Conclusions: In this study indicates that regular resistance exercise to change of leptin and adiponectin might be release of overall decreased ATGL, HSL, MGL and PLIN expression in subcutaneous fatty tissue, and driving reduction of % fat in the obese post- menopausal group more than obese pre- menopausal group.
New structural seismic protection for high-rise building structures
Authors: Kasimzade Azer A. , Abrar Obaidullah , Atmaca Gencay , Kuruoglu Mehmet 
Published: June 2020

Presented Structural Seismic Isolation Method (SSIM) aims to provide high safety for Highly Reliable Structures (HRS) against strong earthquakes including near-fault and long-period ground motions. The examined structure is converted to Structural Seismic Isolation System (SSIS) by the SSIM method which exhibited inverse pendulum behaviour. For this purpose, structure foot base and foundation contact surfaces have been designed as any curved surfaces (spherical, elliptical, etc.) depending on the earthquake-soil-superstructure parameters and this contact surfaces have been separated by elastomeric (lead core rubber or laminated rubber bearings) seismic isolation devices. It would allow the structure foot base to turn around gyration centre through rubber bearing contact and maintains similar behaviour to the super-structure. SSIS system provides the possibility of keeping the natural-period of the structure in a larger interval, which is greater than the predominant-period of the majority of possible earthquakes (including near-fault pulse) using currently existing conventional elastomeric isolators with up to 4 second period. Thus, the structure can sustain its serviceability after strong and long-period earthquakes. In this study SSIS system’s performance is presented for high-rise building structures, for this aim, the finite element model of the building (Bg) structure with SSIS system (SSIS-Bg) has been prepared and the nonlinear dynamic analysis of the model has been conducted using strong and long-period ground motions. Results indicate that the base and top accelerations, base shear and base moment responses of the SSIS-Bg structure is 23.21 %, 75.47 % and 85.74 % in average lower than the Conventional Application Method of Seismic Base Isolation Devices for Building (CAMSBID-Bg) structures respectively and it is not prone to resonant vibrations under long-period earthquakes related with the excessive deformation in the isolation layers in case of using CAMSBID-Bg structures. It should be noted that in this study with the presented SSIM method and SSIS system, it is aimed to protect only the Highly Reliable Structures(HRS) from the effects of strong and long-period ground motions and these structures (HRS) are classified as follows: 1) Nuclear Containment Structures; 2) High-rise buildings that contain information, operating systems, sensitive instruments, communication systems, routing systems, bank operating systems, databases, management systems and other similar facilities that are linked to the security and economy of a country; 3) High-rise hospitals etc.