bluuebunny/crossref_metadata_2025_split huggingface dataset:
Localized Chemogenetic Silencing of Inhibitory Neurons: A novel Mouse Model of Focal Cortical Seizures
Authors: Goldenberg Adi Miriam , Schmidt Sarah , Mitelman Rea , Levy Dana Rubi , Katz Yonatan , Yizhar Ofer , Beck Heinz , Lampl Ilan 
Published: November 2020

AbstractFocal cortical epilepsies are frequently refractory to available anticonvulsant drug therapies. One key factor contributing to this state is the limited availability of animal models that allow to reliably study focal cortical seizures and how they recruit surrounding brain areasin-vivo. In this study, we selectively expressed the inhibitory chemogenetic receptor, hM4D, in GABAergic neurons in focal cortical areas using viral gene transfer. Following focal silencing of GABAergic neurons by administration of Clozapine-N-Oxide (CNO), we demonstrated reliable induction of local epileptiform events in the electroencephalogram (EEG) signal of awake freely moving mice. Experiments in anesthetized mice showed consistent induction of focal seizures in two different brain regions – the barrel cortex (BC) and at the medial prefrontal cortex (mPFC). Seizures were accompanied by high frequency oscillations, a known characteristic of human focal seizures. Seizures propagated, but an analysis of seizure propagation revealed favored propagation pathways. CNO-induced epileptiform events propagated from the BC on one hemisphere to its counterpart and from the BC to the mPFC, but not vice-versa. Lastly, post-CNO epileptiform events in the BC could be triggered by sensory whisker-pad stimulation, indicating that this model, applied to sensory cortices, may be useful to study sensory-evoked seizures. Taken together, our results show that targeted chemogenetic inhibition of GABAergic neurons using hM4D can serve as a novel, versatile and reliable model of focal cortical epilepsy suitable to systematically study cortical ictogenesis in different cortical areas.Significance StatementFocal cortical epilepsies are often hard to alleviate using current anticonvulsant therapies while further drug discovery is impeded by the limited variety of suitable animal models. In this study, we established a novel model of focal cortical seizures induced by spatially-restricted chemogenetic silencing of cortical inhibitory neurons. We have shown this method to be effective at various cortical regions and reliably induce seizures that share key characteristics with known human epilepsy traits, including sensory triggering and seizure propagation. This model may thus be used to advance the discovery of new remedies for focal cortical epilepsies, as well as to improve our understanding of seizure spread along different cortical pathways.
Role of Inferior Frontal Junction (IFJ) in the Control of Feature vs Spatial Attention
Authors: Meyyappan Sreenivasan , Rajan Abhijit , Mangun George R , Ding Mingzhou 
Published: November 2020

ABSTRACTFeature-based attention refers to preferential selection and processing of items and objects based on their non-spatial attributes such as color or shape. While it is intuitively an easier form of attention to relate to in our day to day lives, the neural mechanisms of feature-based attention are not well understood. Studies have long implicated the dorsal attention network as a key control system for voluntary spatial, feature and object-based attention. Recent studies have expanded on this model by focusing on the inferior frontal junction (IFJ), a region in the pre-frontal cortex to be the source of feature attention control, but not spatial attention control. However, the extent to which IFJ contributes to spatial attention remains a topic of debate. We investigated the role of IFJ in the control of feature versus spatial attention in a cued visual spatial (attend left or right) and feature attention (attend red or green) task using fMRI. Analyzing single-trial cue-evoked fMRI responses using univariate GLM and multi-voxel pattern analysis (MVPA), we observed the following. First, the univariate BOLD activation responses yielded no significant differences between feature and spatial cues. Second, MVPA analysis showed above chance level decoding in classifying feature attention (attend-red vs. attend-green) in both the left and right IFJ, whereas during spatial attention (attend-left vs. attend-right) decoding was at chance. Third, while the cue-evoked decoding accuracy was significant for both left and right IFJ during feature attention, target stimulus-evoked neural responses were not different. Importantly, only the connectivity patterns from the right IFJ was predictive of target-evoked activity in visual cortex (V4); this was true for both left and right V4. Finally, the strength of this connectivity between right IFJ and V4 (bilaterally) was found to be predictive of behavioral performance. These results support a model where the right IFJ plays a crucial role in top down control of feature but not spatial attention.
Bromodomain protein inhibition protects β-cells from cytokine-induced death and dysfunction via antagonism of NF-κB pathway
Authors: Negi Vinny , Lee Jeongkyung , Liu Ruya , Perez-Garcia Eliana M. , Li Feng , Jagannathan Rajaganapati , Yang Ping , Bottino Rita , Ma Ke , Moulik Mousumi , Yechoor Vijay K 
Published: November 2020

ABSTRACTCytokine induced β-cell apoptosis is the major pathogenic mechanism in type 1 diabetes (T1D). Despite significant advances in understanding underlying mechanisms, few drugs have been translated to protect β-cells in T1D. Epigenetic modulators such as bromodomain-containing BET (Bromo- and Extra-Terminal) proteins are important regulators of immune responses. Pre-clinical studies have demonstrated a protective effect of BET inhibitors in NOD (non-obese diabetes) mouse model of T1D. However, the role of BET proteins in β-cell function in response to cytokines is unknown. Here we demonstrate that I-BET, a BET protein inhibitor, protected β-cells from cytokine induced dysfunction and death. In vivo administration of I-BET to mice exposed to low-dose STZ (streptozotocin), a model of T1D, significantly reduced β-cell apoptosis and preserved β-cell mass, suggesting a cytoprotective function of I-BET. Furthermore, human islets treated with I-BET displayed better glucose stimulated insulin secretion compared to controls, when exposed to cytokines. Mechanistically, RNA-Seq analysis revealed I-BET treatment suppressed pathways involved in apoptosis, including NF-kB signaling, while maintaining the expression of genes critical for β-cell function, such as Pdx1 and Ins1. Taken together, this study demonstrates that I-BET is effective in protecting β-cells from cytokine-induced dysfunction and apoptosis, and may have potential therapeutic values in T1D.
Influence of color and brightness on ontogenetic shelter preference by prawns (Macrobrachium rosenbergii)
Authors: da Costa Felipe P. , Arruda Maria F. , Ribeiro Karina , Pessoa Daniel M. A. 
Published: November 2020

AbstractThe giant river prawn (Macrobrachium rosenbergii), native to rivers and river mouths of different Asian countries, is a heavily widespread species that has been introduced around the world due to its great commercial importance. These prawns are farmed under many different conditions that might translate to a great range of light environments, which impact their behavior and productivity. Here, as a contribution for prawns’ welfare and economical productivity, we present the first study employing both visual modeling and behavioral data to evaluate ontogenetic changes on color preference of juveniles and adults ofM. rosenbergii. For this purpose, we offered ten shelters of different colors to juveniles and adults and registered their preference. Our results showed that the criterion for shelter preference changed with ontogeny, since juveniles chose shelters based on chromaticity (preference for blue), while adults based their decisions on brightness (preference for dark gray). This preference of adults for dark colors is probably associated with a light avoidance behavior. We recommend providing blue shelters for juveniles and dark shelters for adults.
Aberrant peripheral immune responses in acute Kawasaki disease with single-cell sequencing
Authors: Wang Zhen , Xie Lijian , Song Sirui , Chen Liqin , Li Guang , Liu Jia , Xiao Tingting , Zhang Hong , Huang Yujuan , Ding Guohui , Li Yixue , Huang Min 
Published: November 2020

AbstractKawasaki disease (KD) is the most common cause of acquired heart disease in children in developed countries. Although diverse immune aberrance was reported, a global understanding of immune responses underlying acute KD was lacking. Based on single-cell sequencing, we profiled peripheral blood mononuclear cells from patients with acute KD before and after intravenous immunoglobulin therapy and from healthy controls. Most differentially expressed genes were derived from monocytes, with upregulation of immunoglobulin receptors, complement and receptors and downregulation of MHC class II receptors before therapy. The percentage of B cells was significantly increased before therapy and rapidly returned to normal after therapy. There was also an increased abundance of B-cell receptors withIGHAandIGHGafter therapy, accompanied by massive oligoclonal expansion. The percentage of CD8 T cells was remarkably decreased during acute KD, especially the subset of effector memory CD8 T cells. All lymphocyte compartments were characterized by underexpressed interferon response pathways before therapy. The identification of unique innate and adaptive immune responses suggests potential mechanisms underlying pathogenesis and progression of KD.
Activated Expression of Master Regulator MYB31 and of Capsaicinoid Biosynthesis Genes Results in Capsaicinoid Biosynthesis and Accumulation in the Pericarp of the Extremely PungentCapsicum chinense
Authors: Sun Binmei , Huang Zubing , Wang Juntao , Wei Jianlang , Cai Wen , Yuan Yuan , Zhang Shuangling , Song Jiali , Cao Bihao , Chen Changming , Cao Panrong , Chen Guoju , Lei Jianjun , Zhu Zhangsheng 
Published: November 2020

AbstractCapsaicinoids confer pungency inCapsicumfruits, and the capsaicinoid content varies greatly among the five domesticatedCapsicumspecies. Although it is generally recognized that capsaicinoid biosynthesis occurs exclusively in the placenta, few studies have focused on capsaicinoid biosynthesis gene (CBG) expression in the pericarp. Therefore, the transcriptional regulation mechanisms of capsaicinoid biosynthesis in the pericarp remain elusive. Here, the capsaicinoid contents of 32 accessions from five domesticatedCapsicumspecies were analyzed. The results showed that the capsaicinoid contents ofC. chinenseaccessions are significantly higher than those of the other fourCapsicumspecies due to the increased accumulation of capsaicinoids, especially in the pericarp. Compared to that in accessions with low pericarp capsaicinoid content, the expression of the master regulatorMYB31is significantly upregulated in the pericarp inC. chinenseaccessions, which leads to high levels of CBG expression. Moreover, in fruits of the extremely pungent ‘Trinidad Moruga Scorpion’ (C. chinense) and low-pungency ‘59’ inbred line (C. annuum) at different developmental stages, the capsaicinoid accumulation patterns were consistent with theMYB31and CBG expression levels in the pericarp. Taken together, our results provide novel insights into the molecular mechanism arising from the expression of a master regulator in the pericarp that results in exceedingly hot peppers. The genetic resources identified in this study could be used as genetic resources for the genetic improvement of pepper pungency.
The past is never dead: the role of imprints in shaping social and environmental reporting in a post-communist context
Authors: Albu Nadia , Albu Cătălin Nicolae , Apostol Oana , Cho Charles H. 
Published: November 2020

PurposeMobilizing a theoretical framework combining institutional logics and “imprinting” lenses, this paper provides an in-depth contextualized analysis of how historical imprints affect social and environmental reporting (SER) practices in Romania, a post-communist country in Eastern Europe.Design/methodology/approachThe authors conduct a qualitative field study with a diverse dataset including regulations, publicly available reports and interviews with multiple actors involved in the SER field in Romania. The authors follow a reflexive approach in constructing the narratives by mobilizing their personal experience and understanding of the field to analyze the rich empirical material.FindingsThe authors identify a blend of logics that combine local and Western conceptualizations of business responsibilities and explain how the transition from a communist ideology to the free market economy affected SER practices in Romania. The authors also highlight four major imprints and document their longitudinal development, evidencing three main patterns: persistence, transformation and decay. The authors find that the deep connections that form between logics and imprints explain the cohabitation of logics rather than their straight replacement.Originality/valueThe paper contributes by evidencing the role of imprints' dynamics in the institutionalization of SER logics. The authors claim that the persistence (decay) of imprints from a former regime such as communism hinders (facilitates) the institutionalization of Western SER logics. Transformation instead has more uncertain effects. The pattern that an imprint takes hinges upon its usefulness for business interests.
Fraudulent financial reporting and data analytics: an explanatory study from Ireland
Authors: Aboud Ahmed , Robinson Barry 
Published: November 2020

Purpose
This paper aims to explore the effectiveness of fraud prevention and detection techniques, including data analytics, machine learning and data mining, and to understand how widespread the use of data analytics is across different sectors and to identify and understand the potential barriers to implementing these techniques to detect and prevent fraud.

Design/methodology/approach
A survey was administered to 73 Irish businesses to determine to what extent traditional approach, data mining or text mining are being used to prevent or detect fraudulent financial reporting, and to determine the perception level of their effectiveness.

Findings
The study suggests that whilst data analytics is widely used by businesses in Ireland there is an under-utilisation of data analytics as an effective tool in the fight against fraud. The study suggests there are barriers that may be preventing companies from implementing advanced data analytics to detect financial statement fraud and identifies how those barriers may be overcome.

Originality/value
In contrast to the majority of literature on big data analytics and auditing, which lacks empirical insight into the diffusion, effectiveness and obstacles of data analytics, this explanatory study contributes by providing useful insights from the field on big data analytics. While the extant auditing literature generally addresses the avenues of big data utilisation in auditing domain, our study explores particularly the use big data analytics as a fraud prevention and detection techniques.
Viewpoint – could the belt and road initiative be derailed? An analysis of impediments to completion
Authors: Enderwick Peter 
Published: November 2020

Purpose
The continuation of China’s belt and road initiative (BRI) is assumed in most analyses. Yet, recent events have created significant reputational damage for China and Chinese businesses. With a trade war evolving into a hegemonic struggle, there are a number of potential developments that could derail the BRI. This paper aims to provide a contemporary review of the factors that could negatively impact its continuation, and what China has done to mitigate the risks.

Design/methodology/approach
A descriptive paper that groups possible disruptive factors into three groups: internal weaknesses of the BRI and its design; those related to China’s implementation of the BRI and external concerns and pressures.

Findings
China has actively reviewed and refined the BRI to reduce its perceived weaknesses and increase its attractiveness to potential participants, focussing on debt dependency, transparency and governance. However, this has occurred at the same time as growing concerns regarding China’s international assertiveness, the hegemonic challenge and recovery from the COVID-19 pandemic.

Research limitations/implications
These changes are occurring within an extremely dynamic environment and any analysis at one point in time is subject to considerable limitations. However, the paper brings together a range of disparate perspectives in a structured manner.

Originality/value
The classification of possible threats to the BRI is original and provides insights into the relative significance of the diverse challenges that China faces. The paper concludes that while China’s operational focus on the mechanics of the BRI process is necessary, it may not be sufficient to ensure its continuing development. The paper identifies the next step which is conceptualisation of these ideas and of the BRI. Some guidance as to how this might be done is provided.
Regional and international research collaboration and citation impact in selected sub-Saharan African countries in the period 2000 to 2019
Authors: Onyancha Omwoyo Bosire 
Published: November 2020

PurposeThis paper aims to examine and compares the extent and types of research collaboration and their citation impact in selected countries in sub-Saharan Africa using co-authorship amongst countries as a proxy indicator.Design/methodology/approachThe paper reports the findings of a bibliometric study of publications that were published by authors affiliated to Ethiopia, Ghana, Kenya, Nigeria and Tanzania, between 2000 and 2019 and indexed in the Web of Science’s (WoS) three citation indexes. The social network analysis technique was adopted to articulate collaborative partnerships between and amongst geographical regions. Correlational tests were conducted to gauge the relationship between the frequency and intensity of collaboration and the influence of collaboration on citation impact. The paper highlights the characteristics of country collaborations, the nature of collaboration and the corresponding research impact and relates the types of collaboration to citation impacts in each country.FindingsThe findings reveal that Nigeria and Kenya have had wider and stronger collaborations than Ethiopia, Ghana and Tanzania; the number of collaborating countries has continued to grow in the five countries’ research ecosystems; there are statistically significant relationships between collaboration and citation impact in each country; international collaboration has yielded the most number of citations, with the global North performing better than the South and regional countries; and that the number of citations for the countries more than doubles through research collaboration.Research limitations/implicationsCo-authorship of publications has been faulted but remains the most reliable proxy indicator of research collaboration. The study of the five countries, though depicting patterns of collaboration in many sub-Saharan African countries, cannot be generalised to the entire region.Practical implicationsThe current study has policy implications as far as decisions on research collaboration are concerned. Sub-Saharan African countries and indeed the developing countries may consider re-examining their emphasis on international collaboration to the neglect of domestic and regional collaboration. While the study supports international collaboration, it nevertheless recommends a three-tier collaboration, wherein international collaboration is juxtaposed with domestic and/or regional collaboration.Originality/valueThe study uses social network analysis of country collaboration in developing countries. The intensity and frequency of collaboration are examined in relation to research impact.
Use of lexical items in job interviews by recent graduates in Malaysia
Authors: Amutan Krishnan Isai , Mohd Jan Jariah , Zainuddin Siti Zaidah Binti 
Published: November 2020

PurposeThe purpose of this paper is to explore the knowledge of lexical items in a job interview by recent graduates.Design/methodology/approachThe data were collected from one of the organisations in the Klang Valley, Malaysia. Twenty-seven recent graduates participated in the study. The structured standard interview questions were used to elicit the job interview data. The data were recorded and analysed qualitatively by using Allwood's (1999a, b, c) communicative behaviour theory.FindingsThe findings showed that the lexical items used by the interviewees varied and revealed their knowledge of lexical items in relation to these five characteristics: personality, skills, capability, experience and self-motivation. The successful interviewees reflected their knowledge of lexical items that indicated their confidence in in the job interviews. The lexical items used by the reserved interviewees were limited that displayed their personalities and reflected uncertainty and lack of confidence. The unsuccessful interviewees used limited lexical items and were vague and evasive in answering questions. This could also be not convincing enough to influence the outcome of the interviews positively.Practical implicationsThe findings of this study can assist policymakers such as officials of higher learning institutions to integrate interview workshops and mock interviews into their current curriculum as a form of preparation for undergraduates. These mock interviews can be more appropriate if experienced interviewers are utilised from the outsourcing organisations.Social implicationsIt creates an awareness for job applicants especially undergraduates after completing their studies that knowledge of lexical items are important in job interviews.Originality/valueThe use of appropriate lexical items play an important role in job interviews as they have shown that all the successful interviewees had used them to good effect in their interactions with the interviewer.
The disciplinary research landscape of data science reflected in data science journals
Authors: Hong Lingzi , Moen William , Yu Xinchen , Chen Jiangping 
Published: November 2020

Purpose
This paper aims to selects 59 journals that focus on data science research in 14 disciplines from the Ulrichsweb online repository. This paper analyzes the aim and scope statement using both quantitative and qualitative methods to identify the research types and the scope of research promoted by these journals.

Design/methodology/approach
Multiple disciplines are involved in data science research and publishing, but there lacks an overview of what those disciplines are and how they relate to data science. In this study, this paper aims to understand the disciplinary characteristics of data science research. Two research questions are answered: What is the population of journals that focus on data science? What disciplinary landscape of data science is revealed in the aim and scope statements of these journals?

Findings
Theoretical research is mainly included in journals that belong to statistics, engineering and sciences. Almost all data science journals include applied research papers. Keywords analysis shows that data science research in computers, statistics, engineering and sciences appear to share characteristics. While in other disciplines such as biology, business and education, the keywords are indicative of the types of data to be used and the special problems in these disciplines.

Originality/value
This is the first study to use journals as the unit of analysis to identify the disciplines involved in data science research. The results provide an overview of how researchers and educators from different disciplinary backgrounds understand data science research.