bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the Statistical Testing Methods for Single Laboratory Validation of Qualitative Microbiological Assays with an Unpaired Design
Authors: Wang Shizhen S 
Published: March 2020

AbstractBackgroundThere exists several statistical methods for detecting a difference of detection rates between alternative and reference qualitative microbiological assays in a single laboratory validation study with an unpaired design.ObjectiveWe compared performance of eight methods including Fisher’s exact test, unequal variance two-sample t-test, Wilcoxon rank-sum test, z-test, and methods based on Wilson confidence intervals, complementary log-log regression, Firth’s logistic regression, and ordinary logistic regression.MethodWe first compared the minimum detectable difference in the proportion of detections between the alternative and reference methods among these statistical methods for a varied number of test portions. We then compared power and size of test of these methods using simulated data.ResultsFirth’s logistic regression and the unequal variance two-sample t-test had the lowest minimum detectable difference and highest power. None of these statistical methods had an estimated size of test always within a 95% confidence interval of the nominal value 0.05 with small numbers of test portions (n = 12, 20, 30). Fisher’s exact test, the Wilcoxon rank-sum test, and the z-test were conservative even with a moderately large number of test portions (n = 40), while Firth’s logistic regression and the unequal variance two-sample t-test had a size of test closer to 0.05 than other methods.ConclusionsFirth's logistic regression and the unequal variance two-sample t-test are better choices than other competing methods.HighlightsWe recommend the unequal variance two-sample t-test over Firth’s logistic regression because the unequal variance two-sample t-test is better known and easier to use. We provide an example using real data.
Rapid Method for the Determination of Thiamine and Pantothenic Acid in Infant Formula and Milk-Based Nutritional Products by Liquid Chromatography‒Tandem Mass Spectrometry
Authors: Gill Brendon D , Saldo Sheila C , McGrail Iain J , Wood Jackie E , Indyk Harvey E 
Published: March 2020

Abstract

Background
Thiamine and pantothenic acid play a critical role in numerous metabolic reactions and are typically supplemented in infant and adult nutritional formulas as thiamine chloride hydrochloride and calcium pantothenate salts.

Objective
A rapid compliance method for the analysis of thiamine and pantothenic acid applicable to infant formula and milk-based nutritional products is described.

Method
Proteins are removed by centrifugal ultrafiltration, followed by analysis by reversed-phase liquid chromatography‒tandem mass spectrometry (LC-MS/MS), with quantitation accomplished by internal standard technique.

Results
The method was shown to be accurate, with acceptable recovery (thiamine, 99.3–101.1%; pantothenic acid, 99.2–108.6%). A certified reference material (NIST 1849a), showed no statistical bias (α = 0.05) for thiamine (P = 0.64); although a statistically significant bias (P &lt; 0.01) for pantothenic acid was found, the nominal bias was only 4.7% (mean = 7.1 mg/hg; certified value = 6.8 mg/hg). A comparison of results by LC-MS/MS and current methods showed negligible bias (mean bias: thiamine, 0.01 mg/hg; pantothenic acid, 0.17 mg/hg) and no statistical significance (α = 0.05; thiamine, P = 0.399; pantothenic acid, P = 0.058). Acceptable precision was demonstrated with a repeatability of 7.2% repeatability relative standard deviation (RSDr) (HorRat: 0.6) and an intermediate precision of 7.0% RSD for thiamine, and a repeatability of 5.7% RSDr (HorRat: 0.5) and an intermediate precision of 6.1% RSD for pantothenic acid.

Conclusions
This rapid method is intended for use in high-throughput laboratories as part of routine product compliance release testing of thiamine and pantothenic acid in manufactured infant and milk-based nutritional products.
Development and Validation of a Reversed Phase High Performance Liquid Chromatography-Photodiode Array Detection Method for Simultaneous Identification and Quantification of Coumarin, Precocene-I, β-Caryophyllene Oxide, α-Humulene, and β-Caryophyllene in Ageratum Conyzoides Extracts and Essential Oils from Plants
Authors: Shah Sonal , Dhanani Tushar , Sharma Sonu , Singh Raghuraj , Kumar Satyanshu , Kumar Bhanu , Srivastava Sharad , Ghosh Srikant , Kumar Rajesh , Juliet Sanis 
Published: March 2020

Abstract

Background
Ageratum conyzoides is an aromatic plant. It is considered as an invasive and cosmopolite weed, widely spread in tropical and subtropical regions. Phytochemicals such as benzopyrenes, flavonoids, and terpenoids are reported from A. conyzoides.

Objective
Development and validation of a reversed-phase HPLC-photodiode array (PDA) detection method for simultaneous identification and quantification of coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene in extracts of A. conyzoides and essential oils was carried out.

Methods
Separation of analytes was achieved on a RP-18 (250 mm × 4.6 mm, 5 µm) column using a solvent system comprising of a mixture of acetonitrile and water with 0.05% trifluoroacetic acid in gradient elution mode at ambient temperature with flow rate of 1 mL/min.

Results
The retention time of coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene was 4.38, 12.86, 20.10, 33.34, and 35.11 min, respectively. Limits of detection for coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene were 2.5, 2.5, 2.5, 0.025, and 2.5 µg/mL, respectively. Similarly, LOQ were 10, 10, 10, 0.10, and 10 µg/mL for coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β- caryophyllene, respectively. Repeatabilities (RSD, %) values for intraday and interday precision for coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene was 0.765–2.086 and 0.886–2.128; 0.879–1.672 and 0.979–1.825; 0.696–2.418 and 0.768–2.592; 1.728–2.362 and 1.965–2.378; 1.615–2.897 and 1.658–2.906, respectively.

Conclusions
The separation of five analytes was achieved within 50 min. The developed and validated HPLC-PDA method was successfully applied for identification and quantification of above five analytes in A. conyzoides extracts and essential oils. The method could be used for meeting the characterization criteria of phytoformulations.
Characterization of Insect Stains Deposited by Calliphora vicina (Diptera: Calliphoridae) on Shirt Fabrics
Authors: Rivers David B , Dunphy Brendan , Hammerschmidt Claire , Carrigan Alexandra 
Published: March 2020

AbstractDespite the fact that necrophagous flies are known to alter bloodstains and create unique artifacts, no research has occurred to date that has examined the characteristics of insect stains on textiles or fabrics. This study represents the first effort to characterize artifacts produced by adult Calliphora vicina Robineau-Desvoidy deposited on a range of shirt fabrics that varied in type, color, orientation, and yarn tension. In general, artifact morphology on any type of fabric was distorted in comparison to those observed on smooth and/or nonporous surfaces in previous studies. Consequently, distinction of artifact type could only be made broadly as digestive artifacts and transfer patterns, in which the latter was predominantly detected as tarsal tracks. None of the artifacts displayed satellite stains typical of human bloodstains found on textiles. Wicking was evident on all fabrics but was most pronounced with dri wick and jersey knit polyester in comparison to cotton knit. Digestive artifacts on any colored fabric, but especially with green and yellow shirt samples, resembled the reported color, size, and morphology of bloodstains generated in laboratory studies on a range of fabrics. Unique digestive artifacts were also detected as small, black, and nearly spherical. These defecatory stains did not appear to wet or wick into any of the fabrics. Digestive artifacts and tarsal tracks differentially interacted with front face stitch loops of clothing fabrics to yield distinct stain patterns. The implications of these observations in reference to bloodstain pattern analysis at crime scenes are discussed.
The white dwarf planet WD J0914+1914 b: barricading potential rocky pollutants?
Authors: Veras Dimitri 
Published: March 2020

Abstract
An ice giant planet was recently reported orbiting white dwarf WD J0914+1914 at an approximate distance of 0.07 au. The striking non-detection of rocky pollutants in this white dwarf’s photosphere contrasts with the observations of nearly every other known white dwarf planetary system. Here, I analyse the prospects for exterior extant rocky asteroids, boulders, cobbles, and pebbles to radiatively drift inward past the planet due to the relatively high luminosity ($0.1 \, \mathrm{L}_{\odot }$) of this particularly young (13 Myr) white dwarf. Pebbles and cobbles drift too slowly from Poynting–Robertson drag to bypass the planet, but boulders and asteroids are subject to the much stronger Yarkovsky effect. In this paper, I (i) place lower limits on the time-scales for these objects to reach the planet’s orbit, (ii) establish 3 m as the approximate limiting radius above which a boulder drifts too slowly to avoid colliding with the planet, and (iii) compute bounds on the fraction of boulders that succeed in traversing mean motion resonances and the planet’s Hill sphere to eventually pollute the star. Overall, I find that the planet acts as a barrier against rather than a facilitator for radiatively driven rocky pollution, suggesting that future rocky pollutants would most likely originate from distant scattering events.
RAiSE X: searching for radio galaxies in X-ray surveys
Authors: Turner Ross J , Shabala Stanislav S 
Published: March 2020

ABSTRACT
We model the X-ray surface brightness distribution of emission associated with Fanaroff & Riley type-II radio galaxies. Our approach builds on the RAiSE dynamical model which describes broad-band radio frequency synchrotron evolution of jet-inflated lobes in a wide range of environments. The X-ray version of the model presented here includes: (1) inverse-Compton upscattering of cosmic microwave background radiation; (2) the dynamics of the shocked gas shell and associated bremsstrahlung radiation; and (3) emission from the surrounding ambient medium. We construct X-ray surface brightness maps for a mock catalogue of extended FR-IIs based on the technical characteristics of the eRosita telescope. The integrated X-ray luminosity function at low redshifts (z ≤ 1) is found to strongly correlate with the density of the ambient medium in all but the most energetic sources, whilst at high-redshift (z &gt; 1) the majority of objects are dominated by inverse-Compton lobe emission due to the stronger cosmic microwave background radiation. By inspecting our mock spatial brightness distributions, we conclude that any extended X-ray detection can be attributed to AGN activity at redshifts z ≥ 1. We compare the expected detection rates of active and remnant high-redshift radio AGNs for eRosita and LOFAR, and future more sensitive surveys. We find that a factor of ten more remnants can be detected using X-ray wavelengths over radio frequencies at z &gt; 2.2, increasing to a factor of 100 for redshifts z &gt; 3.1.
Searching for multiple populations in the integrated light of the young and extremely massive clusters in the merger remnant NGC 7252
Authors: Bastian Nate , Lardo Carmela , Usher Christopher , Kamann Sebastian , Larsen Søren S , Cabrera-Ziri Ivan , Chantereau William , Martocchia Silvia , Salaris Maurizio , Asa’d Randa , Hilker Michael 
Published: March 2020

ABSTRACT
Recent work has shown that the properties of multiple populations (MPs) within massive stellar clusters (i.e. in the extent of their abundance variations as well as the fraction of stars that show the anomalous chemistry) depend on the mass as well as the age of the host cluster. Such correlations are largely unexpected in current models for the formation of MPs and hence provide essential insight into their origin. Here, we extend our previous study into the presence or absence of MPs using integrated light spectroscopy of the ∼600 Myr, massive (∼107–108 M⊙) clusters, W3 and W30, in the galactic merger remnant, NGC 7252. Due to the extreme mass of both clusters, the expectation is that they should host rather extreme abundance spreads, manifested through high mean [Na/Fe] abundances. However, we do not find evidence for a strong [Na/Fe] enhancement, with the observations being consistent with the solar value. This suggests that age is playing a key role, or alternatively that MPs only manifest below a certain stellar mass, as the integrated light at all ages above ∼100 Myr is dominated by stars near or above the main-sequence turn-off.
Positive Selection and Inactivation in the Vision and Hearing Genes of Cetaceans
Authors: McGowen Michael R , Tsagkogeorga Georgia , Williamson Joseph , Morin Phillip A , Rossiter and Stephen J 
Published: March 2020

AbstractThe transition to an aquatic lifestyle in cetaceans (whales and dolphins) resulted in a radical transformation in their sensory systems. Toothed whales acquired specialized high-frequency hearing tied to the evolution of echolocation, whereas baleen whales evolved low-frequency hearing. More generally, all cetaceans show adaptations for hearing and seeing underwater. To determine the extent to which these phenotypic changes have been driven by molecular adaptation, we performed large-scale targeted sequence capture of 179 sensory genes across the Cetacea, incorporating up to 54 cetacean species from all major clades as well as their closest relatives, the hippopotamuses. We screened for positive selection in 167 loci related to vision and hearing and found that the diversification of cetaceans has been accompanied by pervasive molecular adaptations in both sets of genes, including several loci implicated in nonsyndromic hearing loss. Despite these findings, however, we found no direct evidence of positive selection at the base of odontocetes coinciding with the origin of echolocation, as found in studies examining fewer taxa. By using contingency tables incorporating taxon- and gene-based controls, we show that, although numbers of positively selected hearing and nonsyndromic hearing loss genes are disproportionately high in cetaceans, counts of vision genes do not differ significantly from expected values. Alongside these adaptive changes, we find increased evidence of pseudogenization of genes involved in cone-mediated vision in mysticetes and deep-diving odontocetes.
Eosinophil and lymphocyte counts predict bevacizumab response and survival in recurrent glioblastoma
Authors: Vaios Eugene J , Winter Sebastian F , Muzikansky Alona , Nahed Brian V , Dietrich Jorg 
Published: March 2020

Abstract

Background
There is a lack of biomarkers to identify glioblastoma (GBM) patients who may benefit from specific salvage therapies, such as the anti-angiogenic agent bevacizumab. We hypothesized that circulating blood counts may serve as biomarkers for treatment response and clinical outcomes.

Methods
Complete blood counts, clinical data, and radiographic information were collected retrospectively from 84 recurrent GBM patients receiving bevacizumab (10 mg/kg every 2 weeks). Significant biomarkers were categorized into quartiles and the association with clinical outcomes was assessed using the Kaplan–Meier method.

Results
The median treatment duration and survival on bevacizumab (OS-A) was 88 and 192 days, respectively. On multivariate analysis, MGMT promoter methylation (hazard ratio [HR] 0.504, P = .031), increases in red blood cells (HR 0.496, P = .035), and increases in eosinophils (HR 0.048, P = .054) during treatment predicted improved OS-A. Patients in the first and fourth quartiles of eosinophil changes had a 12-month survival probability of 5.6% and 41.2% (P &lt; .0001), respectively. Treatment response was associated with increases in eosinophil counts (P = .009) and improved progression-free survival (P = .013). On multivariate analysis, increases in lymphocyte counts among responders predicted improved OS-A (HR 0.389, P = .044). Responders in the first and fourth quartiles of lymphocyte changes had a 12-month survival probability of 0% and 44.4% (P = .019), respectively. Changes in platelet counts differed before and after radiographic response (P = .014).

Conclusions
Changes in circulating eosinophil, lymphocyte, and platelet counts may predict treatment response and clinical outcomes in patients with recurrent GBM receiving bevacizumab.
Anisotropic fluoride nanocrystals modulated by facet-specific passivation and their disordered surfaces
Authors: Yang Ziyu , Zhang Huihui , Xu Junjie , Ma Renzhi , Sasaki Takayoshi , Zeng Yu-Jia , Ruan Shuangchen , Hou Yanglong 
Published: March 2020

Abstract
Rutile-type fluorides have been proven to be active components in the context of emerging antiferr-omagnetic devices. However, controlled synthesis of low-dimensional, in particular two-dimensional (2D), fluorides in a predictable and deterministic manner remains unrealized because of a lack of efficient anisotropic control, which impedes their further development in reduced dimensions. We report here that altered passivation of {110} growing facets can direct the synthesis of rutile-type fluoride nanocrystals into well-defined zero-dimensional (0D) particulates, one-dimensional (1D) rods and 2D sheets in a colloidal approach. The obtained nanocrystals show positive exchange bias and enhanced magnetic transition temperature from the coexistence of long-range antiferromagnetic order and disordered surface spins, making them strong alternatives for flexible magnetic devices and sensors.
De Minimis Normativism: a New Theory of Full Aptness
Authors: Carter J Adam 
Published: March 2020

Abstract
Full aptness is the most important concept in performance-based virtue epistemology. The structure of full aptness, in epistemology and elsewhere, is bilevelled. At the first level, we evaluate beliefs, like performances, on the basis of whether they are successful, competent, and apt—viz successful because competent. But the fact that aptness itself can be fragile—as it is when an apt performance could easily have been inapt—points to a higher zone of quality beyond mere aptness. To break in to this zone, one must not merely perform aptly but also in doing so safeguard in skilled ways against certain risks to inaptness. But how must this be done, exactly? This paper has two central aims. First, I challenge the credentials of mainstream thinking about full aptness by raising some new and serious problems for the view. I then propose a novel account of full aptness—what I call de minimis normativism—which keeps all the advantages of the canonical view, avoids its problems entirely, and offers some additional payoffs.
Linking Pelvic Floor Muscle Function Terminology to the International Classification of Functioning, Disability and Health
Authors: Saltiel Fernanda , Miranda-Gazzola Ana Paula Gonçalves , Vitória Rayane Oliveira , Sampaio Rosana Ferreira , Figueiredo Elyonara Mello 
Published: March 2020

Abstract

Objective
A previous systematic review on pelvic floor muscle function (PFMF) identified a miscellaneity of terms. The lack of consensual terminology might have come from the complexity of neuromusculoskeletal function itself. This study sought to link the previously identified PFMF terms to the International Classification of Functioning, Disability and Health (ICF) terminology.

Methods
In this secondary analysis study, 10 linkage rules were applied to link 196 previously identified PFMF terms to the ICF. Two researchers performed the linking process independently. Disagreements were solved by open dialogue with a third researcher. Percentage agreement was computed for main outcome.

Results
A total of 184 (93.9%) PFMF terms were subsumed into the following 6 ICF terms: tone, involuntary movement reaction, control, coordination, strength, and endurance. The most frequently investigated PFMF was strength (25.5%), followed by involuntary movement reaction (22.9%), endurance (17.2%), control (14.1%), coordination (9.9%), and tone (4.2%). Only 6.2% PFMF could not be linked to ICF terminology. A wide variation of instruments/methods was used to measure PFMF. Vaginal palpation was the only method employed to measure all 6 PFMF. Percentage agreement between raters was 100%.

Conclusions
Linking PFMF terminology to the ICF was feasible and valid. It allowed the identification of the most investigated PFMF and their measuring methods. ICF terminology to describe PFMF should be used since it may improve communication, data gathering, and the advance in scientific knowledge.

Impact
Standardized terminology anchored in a theoretical framework is crucial to data gathering, communication, and dissemination of evidence-based practice. PFMF terminology based on ICF can be used to improve data pooling and communication.
Targeted Pathway-basedIn VivoTesting Using Thyroperoxidase Inhibition to Evaluate Plasma Thyroxine as a Surrogate Metric of Metamorphic Success in Model AmphibianXenopus laevis
Authors: Haselman Jonathan T , Olker Jennifer H , Kosian Patricia A , Korte Joseph J , Swintek Joseph A , Denny Jeffrey S , Nichols John W , Tietge Joseph E , Hornung Michael W , Degitz Sigmund J 
Published: March 2020

AbstractChemical safety evaluation is in the midst of a transition from traditional whole-animal toxicity testing to molecular pathway-based in vitro assays and in silico modeling. However, to facilitate the shift in reliance on apical effects for risk assessment to predictive surrogate metrics having characterized linkages to chemical mechanisms of action, targeted in vivo testing is necessary to establish these predictive relationships. In this study, we demonstrate a means to predict thyroid-related metamorphic success in the model amphibian Xenopus laevis using relevant biochemical measurements during early prometamorphosis. The adverse outcome pathway for thyroperoxidase inhibition leading to altered amphibian metamorphosis was used to inform a pathway-based in vivo study design that generated response-response relationships. These causal relationships were used to develop Bayesian probabilistic network models that mathematically determine conditional dependencies between biochemical nodes and support the predictive capability of the biochemical profiles. Plasma thyroxine concentrations were the most predictive of metamorphic success with improved predictivity when thyroid gland sodium-iodide symporter gene expression levels (a compensatory response) were used in conjunction with plasma thyroxine as an additional regressor. Although thyroid-mediated amphibian metamorphosis has been studied for decades, this is the first time a predictive relationship has been characterized between plasma thyroxine and metamorphic success. Linking these types of biochemical surrogate metrics to apical outcomes is vital to facilitate the transition to the new paradigm of chemical safety assessments.