bluuebunny/crossref_metadata_2025_split huggingface dataset:
Habitat Suitability Study of Hylomecon Japonica Based on Maxent Model in China
Authors: Zhen Cao , Xiaoyan Zhang , Xuanji Xue , Lei Zhang , Guanqun Zhan , Xinxin Zhang , Zengjun Guo 
Published: September 2020

Abstract
Background: To understand the potential distribution and habitat suitability of H. japonica in China. And to provide guidance for the wild cultivation and standardized planting of H. japonica. Methods: The maximum entropy model (Maxent) and geographic information system (ArcGIS) were applied to predict the potential suitable habitat of H. japonica species, and the contribution of variables were evaluated by using the jackknife test. Results: The AUC value confirmed the accuracy of the model prediction based on 101 occurrence records. The potential distributions of H. japonica were mainly concentrated in Jilin, Liaoning, Shaanxi and other provinces (adaptability index>0.6). Jackknife experiment showed that the precipitation of driest month (35.6%), precipitation of wettest quarter (13.4%), the mean annual temperature (7.8%) and the subclass of soil (7.8%) were the most important factors affecting the potential distribution of H. japonica. Conclusion: The niche parameters of the most suitable growth area (adaptability index>0.8) for H. japonica were precipitation of driest month (5 mm), precipitation of wettest quarter (400-490 mm), the mean annual temperature (-2-4 °C) and the subclass of soil (Glossy Chernozem, Gleyic Lime, Haplic Gypsisols).
Preventive Antibiotic Treatment Increases Neonatal Chicks Susceptibility to Salmonella Infection Via Disrupting Gut Microbiota and Linoleic Acid Metabolism
Authors: Mei Xueran , Ma Boheng , Zhai Xiwen , Zhang Anyun , Lei Changwei , Zuo Lei , Tang Yizhi , Yang Xin , Gao Yufeng , Zhou Changyu , Wang Hongning 
Published: September 2020

Abstract
Background: Antimicrobial agents have been widely used in animal farms to prevent and treat animal diseases. However, antimicrobial agents may change the bacterial community and increase susceptibility to the pathogenic bacteria infection. Here, we used metagenomic and metabonomic approach to investigate the effects of florfenicol (FFC) pre-treatment on colonization of Salmonella enterica serovar Enteritidis (S. Enteritidis) in intestines of neonatal chicks through analysis of host responses, microbiota and metabolic changes. Results: We observed that FFC pre-treatment significantly increases the level of S. Enteritidis in the cecal contents, spleen and liver and also induces changes to the cecal microbiota and metabolism. Prior to S. Enteritidis infection, FFC significantly reduced the content of Lactobacillus, and significantly affected the linoleic acid metabolism pathway, including significantly reducing the levels of conjugated linoleic acid (CLA), and significantly increasing the abundance of 12,13-EpOME and 12,13-diHOME in cecum. After infection with S. Enteritidis, the abundance of Proteobacteria were significantly increased and the Salmonella-induced intestinal inflammatory responses and intestinal barrier damage were exacerbated. Supplementation with CLA could maintain intestinal integrity, reduce intestinal inflammation, and directly inhibit Salmonella growth to effectively reduce the Salmonella colonization, whereas the 12,13-diHOME through promoting intestinal inflammation and destroying the intestinal barrier function to support the Salmonella infection. Conclusions: Overall, FFC can decrease levels of Lactobacillus and CLA, and elevate cecal 12,13-diHOME concentrations in neonatal chicks and thereby increases susceptibility to Salmonella infection. This study revealed a potential health impact of antibiotics and disturbed gut microbiota and linoleic acid metabolism might be an intestinal health-impairing attribute and may contribute to Salmonella colonization.
Establishment of a Duplex Real-time qPCR Method for Detection of Salmonella Spp. And Serratia Fonticola in Fishmealestablishment of a Duplex Real-time qPCR Method for Detection of Salmonella Spp. And Serratia Fonticola in Fishmeal
Authors: Ruan Jinghua , Wang Wujun , Zhang Tiying , Zheng Teng , Zheng Jing , Yu Shiyu , Yu Daojin , Huang Yifang 
Published: September 2020

Abstract
Salmonella spp. is a high-risk bacterial pathogen that is monitored in imported animal-derived feedstuffs. Serratiafonticola is the bacterial species most frequently confused with Salmonella spp. in traditional identification methods based on biochemical characteristics, which are time-consuming and labor-intensive, and thus unsuitable for daily inspection and quarantine work. In this study, we established a duplex real-time qPCR method with invA- and gyrB-specific primers and probes corresponding to Salmonella spp. and S. fonticola. The method could simultaneously detect both pathogens in imported feedstuffs, with a minimum limit of detection for Salmonella spp. and S. fonticola of 197 copies/SL and 145 copies/SL, respectively (correlation coefficient R2 = 0.999 in both cases). The amplification efficiency for Salmonella spp. and S. fonticola was 98.346% and 96.49%, respectively. Detection of clinical samples was consistent with method GB/T 13091-2018, and all seven artificially contaminated imported feed samples were positively identified. Thus, the developed duplex real-time qPCR assay displays high specificity and sensitivity, and can be used for the rapid and accurate detection of genomic DNA from Salmonella spp. and S. fonticola within hours. This represents a significant improvement in the efficiency of detection of both pathogens in imported feedstuffs.
Descriptive Study of a 488 Patients Cohort with Inherited Retinal Diseases and a Low Genetic Diagnosis Rate.
Authors: Coco-Martin Rosa M. , Diego-Alonso Miguel , Orduz-Montaña W. Andres , Sanabria M. Rosa , Sanchez-Tocino Hortensia 
Published: September 2020

Abstract
Backgorund: To analyze the distribution of inherited retinal diseases (IRDs), describe the clinical characteristics of patients, and determine the percentages of patients with genetic diagnoses in the Castilla and Leon region of Spain.Methods: All patients with an IRD seen in the two major referral units of Castilla y Leon during a 20-year period were included. The ages at symptom onset, diagnosis, and the last visit; sex; family history; history of consanguinity; type of inheritance; status of the fundus and electroretinogram; lens and macular status, visual acuity; and visual field data were recorded. Patients were divided into those with retinitis pigmentosa (RP) and all others. The genetic mutations were gathered.Results: Four hundred eighty-eight patients with IRDs were studied, 216 with RP (of which 15.74% had syndromic disease) and 272 with other conditions (59.19% of which were macular dystrophies). A genetic study had been performed in 27.31% of patients (29.31% of which were negative) among the patients with RP and in 26.1% of the rest of the IRDs (28.16% of which were negative). In the group without a genetic diagnosis, 70.6% of subjects had good remaining vision. The mean delay in diagnosis was 6-16.2 years.Conclusions: The percentage of patients with IRDs that are candidates for undergoing personalized treatments including gene therapy in our region is low and must be improved. Most patients with RP who did not undergo a genetic study have good remaining vision, so they would be candidates for receiving treatments aimed at maintaining vision. There is a significant delay in diagnosis.
TreeSke: A Structural-Lossless Skeleton Extraction Method for Point Cloud Data of Canopy Woody Materials
Authors: Wu Bingxiao , Zheng Guang , Chen Yang , Yu Dongsheng 
Published: September 2020

Abstract
Background: Skeletons extracted from point clouds of woody materials present canopy structural features (e.g., the inclination angle of branches) for simulating canopy interception and understory solar radiation distribution. However, existing methods cannot easily capture structure-lossless skeletons of woody organs from tree point-cloud data. To fulfill this goal, we proposed a distance-weighted method, named the TreeSke method, to iteratively contract the point cloud of canopy woody materials to their median-axis skeleton. After heuristically searching the local point set, we pointwise-extracted the tightened weights to obtain the coarse skeleton. Then, we thinned the skeleton of woody organs and optimized it by a noise filtering and re-centering process. Results: The proposed method was verified on six simulated tree models that have reference skeletons and two field-collected datasets at the plot level. The results show that the TreeSke-extracted skeletons were with higher location accuracy than using the other two tested methods. The mean offset distance, the RMSE of the offset distance, and the Hausdorff distance between the extracted and reference skeletons were less than 0.04 m, less than 0.06 m, and less than 0.11 m, respectively. Conclusions: The extracted skeletons by the TreeSke method could nearly integrally depict the structural features of woody materials. The proposed method showed robust for noisy points and outliers, while missing data would reduce the skeleton integrity and cause deformation errors. From the extracted skeletons, users can extract the inclination-angle features of canopy woody materials, which are useful for simulating most eco-hydrological processes.
Improved pyrrolysine biosynthesis through continuous directed evolution of the complete pathway
Authors: Ho Joanne , Miller Corwin , Mattia Jacob , Bennett Matthew 
Published: September 2020

Abstract
Pyrrolysine (Pyl, O) exists in nature as the 22nd proteinogenic amino acid. Despite being a fundamental building block of proteins, studies of Pyl have been hindered by the difficulty and inefficiency of both its chemical and biological syntheses. Here, we improved Pyl biosynthesis via rational engineering and directed evolution of the entire biosynthetic pathway. To accommodate toxicity of Pyl biosynthetic genes in Escherichia coli, we devised an approach termed Alternating Phage Assisted Non-Continuous Evolution (Alt-PANCE) that alternates mutagenic and selective phage growths. The evolved pathway exhibited a 32-fold improved yield of Pyl-containing super- folder green fluorescent protein (sfGFP) compared to the rationally engineered ancestor, whereas the WT pathway produced no detectable quantities of Pyl-containing sfGFP. This study demonstrates that Alt-PANCE provides a general approach for evolving proteins exhibiting toxic side effects, and further provides an improved pathway capable of producing substantially greater quantities of Pyl- proteins in E. coli.
Salmon Gut Microbiota Correlates With Disease Infection Status: Potential for Monitoring Health in Farmed Animals&nbsp;&nbsp;
Authors: Bozzi Davide , Rasmussen Jacob A. , Carøe Christian , Sveier Harald , Nordøy Kristian , Gilbert M. Thomas P. , Limborg Morten T. 
Published: September 2020

Abstract
Background: Infectious diseases cause significant production losses in aquaculture every year. Given the pivotal role played by the gut microbiota in regulating the host immune system, health and physiology, interest has risen in the possibility of controlling the fish health status by modulating the gut microbiota. An altered gut microbiota is often associated with a disease status. However, few studies have examined the association between disease severity and degree of gut dysbiosis, especially when the gut is not the site of the primary infection. Moreover, there is a lack of knowledge on the impact of formalin, a commonly used disinfectant in aquaculture, on the gut microbiome. Here we investigate, through 16S rRNA gene metabarcoding, changes in the distal gut microbiota composition of a captive-reared cohort of Atlantic salmon (Salmo salar L.), in consequence of an external bacterial skin infection and the subsequent formalin treatment.Results: We show that the distal gut of diseased salmon presented a different composition from that of healthy individuals, with an increased relative abundance of strains regarded as opportunistic in sick fish. Conversely, healthy salmon were dominated by a new, yet undescribed, Mycoplasma genus. We also observed a positive correlation between fish weight and Mycoplasma sp. relative abundance, potentially indicating a beneficial effect for its host. Moreover, we observed that the treatment with formalin, while suited to resolve the external infection, was unable to recover the gut microbiota characteristic of healthy fish prior to treatment, potentially compromising the subsequent health status and growth performances of all treated fish.Conclusions: We conclude that infectious diseases have the potential of affecting the host gut microbiota at different body sites and that treatment optimization procedures should account for that. The formalin treatment is not an optimal solution from a holistic perspective, we suggest its coupling with a probiotic treatment aimed at re-establishing the original community. Lastly, we have observed a positive correlation of Mycoplasma sp. with salmon health and growth performances and, while inviting further research on this microorganism, we also encourage further investigations towards its potential utilization as a biomarker for monitoring health in salmon and potentially other farmed fish species.
The Proposal and Research on the Most Basic Problem of Fault Diagnosis
Authors: Jun Hu 
Published: September 2020

Abstract
By keep asking “what happened to the part that was connected between normal and fault?” The basic problem of fault diagnosis is put forward. Based on the parameter analysis, the most basic problem of fault diagnosis is pointed to the generalized stiffness looseness through the congenital underlying logic presupposition of the fault. The relationship between generalized looseness, part fault and system fault is analyzed by analytic method, which further proves that looseness fault is the most basic problem in fault diagnosis.Finally, the essential priority of looseness fault in fault diagnosis is expounded.The essential priority of loosening fault in fault diagnosis explains the basic problem of fault diagnosis and provides a scientific basis for the systematic development of fault diagnosis.
Multi-Attribute Access Selection Algorithm for Heterogeneous Wireless Networks Based On Uncertain Network Attribute Values
Authors: Liang Gen , Guo Xiaoxue , Sun Guoxi , Fang Jingcheng , Yu Hewei , Lin Maoyuan 
Published: September 2020

Abstract
A heterogeneous wireless network (HWN) environment contains many kinds of wireless networks, such as UMTS, LTE, and WLAN, where users move around within their coverage area. How to ensure mobile users select the most suitable network is a hot research topic for HWNs. While traditional access selection algorithms assume that mobile users can obtain accurate network attribute values, the network attribute values obtained by mobile users are often uncertain due to the mobility of users, the interference of wireless signals, and the fluctuation of the network state. To solve this problem, this paper designs an access selection algorithm for HWNs in the context of inaccurate network attribute values. First, the algorithm calculates the network attribute values based on the hesitant fuzzy theory, then calculates the weights of network attributes using the fuzzy analytic hierarchy process (FAHP), and finally sorts the candidate networks using the hesitant fuzzy technique for order preference by similarity to ideal solution (TOPSIS) method. The simulation results show the proposed algorithm enables users to select the most suitable network to access under the inaccurate network attribute environment and obtain higher gains.
Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering
Authors: Chato-Astrain Jesus , Chato-Astrain Isabel , Sanchez-Porras David , Garcia-Garcia Oscar-Dario , Bermejo-Casares Fabiola , Vairo Claudia , Villar-Vidal María , Gainza Garazi , Villullas Silvia , Oruezabal Roke Iñaki , Ponce Angela , Garzon Ingrid , Carriel Victor , Campos Fernando , Alaminos Miguel 
Published: September 2020

Abstract
Background: Treatment of patients affected by severe burns is challenging, especially due to the high risk of Pseudomonas infection. In the present work, we have generated a novel model of bioartificial human dermis substitute by tissue engineering to treat infected wounds using fibrin-agarose biomaterials functionalized with nanostructured lipid carriers (NLCs) loaded with two anti-Pseudomonas antibiotics: sodium colistimethate (SCM) and amikacin (AMK). Results: Results show that the novel tissue-like substitutes have strong antibacterial effect on Pseudomonas cultures, directly proportional to the NLC concentration. Free DNA quantification, WST-1 and Caspase 7 immunohistochemical assays in the functionalized dermis substitute demonstrated that neither cell viability nor cell proliferation were affected by functionalization in most study groups. Furthermore, immunohistochemistry for PCNA and KI67 and histochemistry for collagen and proteoglycans revealed that cells proliferated and were metabolically active in the functionalized tissue with no differences with controls. When functionalized tissues were biomechanically characterized, we found that NLCs were able to improve some of the major biomechanical properties of these artificial tissues, although this strongly depended on the type and concentration of NLCs. Conclusions: These results suggest that functionalization of fibrin-agarose human dermal substitutes with antibiotic-loaded NLCs is able to improve the antibacterial and biomechanical properties of these substitutes with no detectable side effects. This opens the door to future clinical use of functionalized tissues.
Traditional Medicinal vegetables in Northern Uganda. An ethnobotanical survey.
Authors: Nakaziba Rebecca , Anyolitho Maxson Kenneth , Amanya Sharon Bright , Ogwal-Okeng Jasper , Alele Paul E. 
Published: September 2020

Abstract
Background A wide range of indigenous vegetables grow in Uganda especially during rainy seasons but scarcely during droughts, except those that are commercially grown. Although a number of these vegetables have medicinal values, they have not been satisfactorily studied besides conservation. Therefore, we conducted an ethnobotanical cross-sectional survey in Northern Uganda in order to document traditional vegetables and their medicinal values. Methods An ethnobotanical survey was conducted in Northern Uganda using both qualitative and quantitative data collection approaches to data collection and analysis. Data was collected using semi-structured, interviewer-administered questionnaires following international ethical codes and key informant interviews using well developed key informant guides. A total of 244 individuals participated in the study. Results The study documented forty five (45) traditional vegetables in Northern Uganda, out of which fifteen (15) also served as folk medicine. These included: Amalakwang (Hibiscus sabdariffa), Akeo (Gynandropsis gynandra), Alaju (Asystasia gangetica), Otigo (Corchorus spp.), Ocwica (Cucubita maxima), and Bojo (Vigna unguiculata) among others. The disease conditions managed using traditional vegetables in the study area included: gastrointestinal, reproductive, musculoskeletal complications as well as non-communicable diseases.Conclusion Northern Uganda has numerous traditional vegetables with medicinal benefits such as gastrointestinal, reproductive and musculoskeletal abnormalities. The community obtains vegetable leaves from the backyard and stews them for the medicinal purposes. However, there was no specific dosage administered. Therefore, we recommend studies to standardize the dosages and verify in laboratory models the efficacy of these vegetables.
The effects of meditation on length of telomeres in healthy individuals: A systematic review
Authors: Dasanayaka Nirodhi Namika , Sirisena Nirmala , Samaranayake Nilakshi 
Published: September 2020

Abstract
Background: Meditation based practices have been suggested to result in many biological benefits which include reduction of attrition of telomeres, the protective nucleotide-protein complexes at termini of eukaryotic chromosomes. This systematic review evaluated the effects of meditation on telomere length (TL) in healthy adults. Methods: Randomized controlled trials (RCTs) and Case-control studies (CCS) conducted to determine the effects of meditation on TL in healthy individuals, published up to July 2020 were retrieved by searching seven electronic databases (PubMed, Scopus, PsycINFO, Embase, Cochrane Library, CINAHL and Google Scholar). The methodological quality of RCTs and CCS was assessed using the Cochrane Collaboration Risk of Bias Tool and Joanna Briggs Institute critical appraisal checklist respectively. The data was synthesized narratively and the effect estimates of telomere length in the RCTs was synthesized using alternative methods as a meta-analysis was not conducted. The certainty of evidence was classified according to the GRADE system. Results: A total of 1751 articles were screened. Five studies comprising two RCTs and three CCS were included in the final review based on the inclusion and exclusion criteria. The combined sample consisted of 615 participants with 41.7% males. Average age of participants was 47.7 years. One CCS and one RCT reported significant beneficial effects of meditation on TL while the two remaining CCS and the RCT showed positive effects of meditation on TL which were not significant. For all CCS and one RCT, the methodological quality was high while the remaining RCT was of moderate quality. The quality of evidence for the primary outcome was moderate in RCTs.Conclusion: The present review adds to the existing evidence showing that meditation is potentially beneficial in reducing shortening of TL in healthy adults. However, strictly designed and well-reported RCTs with larger sample sizes are required to provide evidence of higher quality. Systematic Review Registration: The protocol of this review was registered with the International Prospective Register of Systematic Reviews (PROSPERO) database (registration number: CRD42020153977)
Generalised Odd Frechet Family of Distributions: Properties and Applications
Authors: Marganpoor Shahdie , Ranjbar Vahid , Alizadeh Morad , Abdollahnezhad Kamel 
Published: September 2020

Abstract
A new distribution called Generalized Odd Fréchet (GOF) distribution is presented and its properties explored. Some structural properties of the proposed distribution, including the shapes of the hazard rate function, moments, conditional moments, moment generating function, skewness, and kurtosis are presented. Mean deviations, Lorenz and Bonferroni curves, Rényi entropy, and the distribution of order statistics are given. The maximum likelihood estimation technique is used to estimate the model parameters, and finally applications of the model to a real data set are presented to illustrate the usefulness of the proposed distribution.