bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recolonizing wolves and opportunistic foxes: interference or facilitation?
Authors: Ferretti Francesco , Pacini Giada , Belardi Irene , ten Cate Bouke , Sensi Marco , Oliveira Raquel , Rossa Mariana , Burrini Lucia , Lovari Sandro 
Published: August 2020

AbstractThe mechanisms of interactions among carnivore species range from facilitation (mainly through increased availability of prey carcasses) to competition. We assessed the potential for facilitative/competitive interactions between the two most widespread carnivores in the world, the wolf and the red fox, in a prey-rich area recently recolonized by the apex predator. One could expect that the superior competitor would ecologically suppress the inferior one, leading to avoidance of the former by the latter. In a Mediterranean coastal area (2017–2018), we assessed spatiotemporal and dietary interspecific overlap and investigated whether the recovery of wolves affected food habits of foxes. Spatiotemporal overlap was extensive (0.84–0.89). Wild ungulates were the staple of the wolf diet (~88–90%); foxes used mainly invertebrates and fruits (~78%), with ungulates being a substantial food category (13% of diet; 66% of occurrences among vertebrate prey). Interspecific dietary overlap was low (0.23), but extensive (0.89) for vertebrate prey. In comparison to a preceding wolf-free period, the volume and occurrence of large mammals in the diet of foxes showed a 2.8- to 3.5-fold increase. Apparently, foxes did not avoid wolves, which provided additional food to the foxes as prey leftovers. In a rich community, the presence of wolves may increase the food spectrum of foxes. Temporal variation of facilitation vs. competition should be assessed in relationship to spatiotemporal changes of predator–prey numbers.
Rebuilding
Authors: Jackson Rob 
Published: August 2020

Abstract
Enriching BioScience's role as a Forum for Integrating the Life Sciences, Arts in Science provides an occasional venue for poems, visual art, and other forms of artistic expression that explore and enliven our understanding of life. Through the contributions in this section, we hope to share with our readers the passion for nature that science inspires.
These contributions are from Rob Jackson, professor, Earth System Science; Senior Fellow, Stanford Woods Institute for the Environment; and Senior Fellow, Precourt Institute for Energy, at Stanford University, in California.
Clinical Practice Guidelines by the Infectious Diseases Society of America (IDSA), American Academy of Neurology (AAN), and American College of Rheumatology (ACR): 2020 Guidelines for the Prevention, Diagnosis and Treatment of Lyme Disease
Authors: Lantos Paul M , Rumbaugh Jeffrey , Bockenstedt Linda K , Falck-Ytter Yngve T , Aguero-Rosenfeld Maria E , Auwaerter Paul G , Baldwin Kelly , Bannuru Raveendhara R , Belani Kiran K , Bowie William R , Branda John A , Clifford David B , DiMario Francis J , Halperin John J , Krause Peter J , Lavergne Valery , Liang Matthew H , Meissner H Cody , Nigrovic Lise E , Nocton James (Jay) J , Osani Mikala C , Pruitt Amy A , Rips Jane , Rosenfeld Lynda E , Savoy Margot L , Sood Sunil K , Steere Allen C , Strle Franc , Sundel Robert , Tsao Jean , Vaysbrot Elizaveta E , Wormser Gary P , Zemel Lawrence S 
Published: August 2020

AbstractThis evidence-based clinical practice guideline for the prevention, diagnosis, and treatment of Lyme disease was developed by a multidisciplinary panel representing the Infectious Diseases Society of America (IDSA), the American Academy of Neurology (AAN), and the American College of Rheumatology (ACR). The scope of this guideline includes prevention of Lyme disease, and the diagnosis and treatment of Lyme disease presenting as erythema migrans, Lyme disease complicated by neurologic, cardiac, and rheumatologic manifestations, Eurasian manifestations of Lyme disease, and Lyme disease complicated by coinfection with other tick-borne pathogens. This guideline does not include comprehensive recommendations for babesiosis and tick-borne rickettsial infections, which are published in separate guidelines. The target audience for this guideline includes primary care physicians and specialists caring for this condition such as infectious diseases specialists, emergency physicians, internists, pediatricians, family physicians, neurologists, rheumatologists, cardiologists and dermatologists in North America.
Palpitation referrals from primary care to a secondary care cardiology outpatient clinic: assessing adherence to guidelines
Authors: Pallikadavath Susil , Patel Roshan , Sarania Rishi , Ahmad Talal , Vali Zakariyya , Kandala Ngianga II , Sandilands Alastair 
Published: August 2020

Abstract

Background and aims
Palpitations are a common presentation in primary care. Guidelines have been developed to identify patients with palpitations who require further assessment by a cardiologist in secondary care. However, patients that do not meet guideline thresholds for referrals are still referred to secondary care services. This audit evaluated the adherence to referral guidelines at our trust and assessed the characteristics of patients who were referred appropriately versus those referred without meeting guideline referral thresholds (inappropriate referral).

Results
Palpitation referrals to a single cardiology outpatient clinic were assessed (n = 66). Half the patients referred for palpitations were referred inappropriately (n = 34, 51.5%). Patients referred inappropriately were more likely to have a benign diagnosis after assessment (91.2%). These patients also had significantly fewer investigations [mean difference of 1.1 (confidence interval: 0.6–1.6)]. Specialist investigations, such as cardiac event recorders (P &lt; 0.05) and cardiac magnetic resonance imaging (P &lt; 0.05) were less likely to be used in inappropriately referred patients.

Conclusions
The results from this audit provide early evidence that there are a significant number of patients who are being referred that could be managed in primary care. Further studies are needed to confirm our findings in larger cohorts and to establish the underlying reasons for inappropriate referrals.
Human-specific microglial Siglec-11 transcript variant has the potential to affect polysialic acid-mediated brain functions at a distance
Authors: Hane Masaya , Chen Dillon Y , Varki Ajit 
Published: August 2020

AbstractCD33-related Siglecs are often found on innate immune cells and modulate their reactivity by recognition of sialic acid-based “self-associated molecular patterns” and signaling via intracellular tyrosine-based cytosolic motifs. Previous studies have shown that Siglec-11 specifically binds to the brain-enriched polysialic acid (polySia/PSA) and that its microglial expression in the brain is unique to humans. Furthermore, human microglial Siglec-11 exists as an alternate splice form missing the exon encoding the last (fifth) Ig-like C2-set domain of the extracellular portion of the protein, but little is known about the functional consequences of this variation. Here, we report that the recombinant soluble human microglial form of Siglec-11 (hSiglec-11(4D)-Fc) binds endogenous and immobilized polySia better than the tissue macrophage form (hSiglec-11(5D)-Fc) or the chimpanzee form (cSiglec-11(5D)-Fc). The Siglec-11 protein is also prone to aggregation, potentially influencing its ligand-binding ability. Additionally, Siglec-11 protein can be secreted in both intact and proteolytically cleaved forms. The microglial splice variant has reduced proteolytic release and enhanced incorporation into exosomes, a process that appears to be regulated by palmitoylation of cysteines in the cytosolic tail. Taken together, these data demonstrate that human brain specific microglial hSiglec-11(4D) has different molecular properties and can be released on exosomes and/or as proteolytic products, with the potential to affect polySia-mediated brain functions at a distance.
Is IV iron sucrose a cost-effective option for treatment of severe anaemia in pregnancy as compared with oral iron?
Authors: Ray Shomik , Neogi Sutapa B , Singh Ranjana , Devasenapathy Niveditha , Zodpey Sanjay 
Published: August 2020

AbstractAnaemia in pregnancy is a public health concern because it is strongly associated with maternal and perinatal morbidity and mortality. An open label randomized controlled trial (RCT) was conducted in India across four government medical colleges, comparing intravenous (IV) iron sucrose and oral iron for the treatment of anaemia in pregnancy. This RCT failed to demonstrate superiority of IV iron sucrose compared with oral iron therapy in reducing adverse clinical (maternal and foetal/neonatal) outcomes in moderate-to-severe anaemia in pregnancy. However, IV iron sucrose seemed to reduce the need for blood transfusion among women with severe anaemia. The study objective was to conduct a cost-effectiveness analysis of IV iron sucrose over oral therapy for treatment of severe anaemia in pregnancy, alongside the RCT, to inform policy. The outcome of interest in our study was a ‘safe delivery’ defined by the absence of composite maternal and foetal/neonatal adverse clinical outcomes. Incremental cost-effectiveness ratio (ICER) was calculated from a limited societal perspective. IV iron sucrose was found to be more costly but more effective than the oral therapy for treatment of severe anaemia. The ICER was calculated at INR 31 951 (USD 445.2) per safe delivery. We considered a threshold of half the gross national income for decision-making. Considering this threshold of India (INR 57 230, USD 797.4), IV iron-sucrose remained cost-effective in 67% of the iterations in the model. At the current ICER, for every 32 severely anaemic pregnant woman treated with IV iron sucrose one additional pregnant woman will have a safe delivery. Such analyses can complement the national strategy to support evidence-based action.
Integrative analysis of scRNA-seq and GWAS data pinpoints periportal hepatocytes as the relevant liver cell types for blood lipids
Authors: Hao Xingjie , Wang Kai , Dai Chengguqiu , Ding Zeyang , Yang Wei , Wang Chaolong , Cheng Shanshan 
Published: August 2020

Abstract
Liver, a heterogeneous tissue consisting of various cell types, is known to be relevant for blood lipid traits. By integrating summary statistics from genome-wide association studies (GWAS) of lipid traits and single-cell transcriptome data of the liver, we sought to identify specific cell types in the liver that were most relevant for blood lipid levels. We conducted differential expression analyses for 40 cell types from human and mouse livers in order to construct the cell-type specifically expressed gene sets, which we refer to as construction of the liver cell-type specifically expressed gene sets (CT-SEGS). Under the assumption that CT-SEGS represented specific functions of each cell type, we applied stratified linkage disequilibrium score regression to determine cell types that were most relevant for complex traits and diseases. We first confirmed the validity of this method (of delineating functionally relevant cell types) by identifying the immune cell types as relevant for autoimmune diseases. We further showed that lipid GWAS signals were enriched in the human and mouse periportal hepatocytes. Our results provide important information to facilitate future cellular studies of the metabolic mechanism affecting blood lipid levels.
The effects of eight serum lipid biomarkers on age-related macular degeneration risk: a Mendelian randomization study
Authors: Han Xikun , Ong Jue-Sheng , Hewitt Alex W , Gharahkhani Puya , MacGregor Stuart 
Published: August 2020

Abstract

Background
Age-related macular degeneration (AMD) is a leading cause of vision loss. Whereas lipids have been studied extensively to understand their effects on cardiovascular diseases, their relationship with AMD remains unclear.

Methods
Two-sample Mendelian randomization (MR) analyses were performed to systematically evaluate the causal relationships between eight serum lipid biomarkers, consisting of apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), total cholesterol (CHOL), high-density lipoprotein cholesterol (HDL-C), direct low-density lipoprotein cholesterol (LDL-C), lipoprotein A [Lp(a)], triglycerides (TG) and non-HDL cholesterol (non-HDL-C), and the risk of different AMD stages and subtypes. We derived 64–407 genetic instruments for eight serum lipid biomarkers in 419 649 participants of European descent from the UK Biobank cohort. We conducted genome-wide association studies (GWAS) for 12 711 advanced AMD cases [8544 choroidal neovascularization (CNV) and 2656 geographic atrophy (GA) specific AMD subtypes] and 5336 intermediate AMD cases with 14 590 controls of European descent from the International AMD Genomics Consortium.

Results
Higher genetically predicted HDL-C and ApoA1 levels increased the risk of all AMD subtypes. LDL-C, ApoB, CHOL and non-HDL-C levels were associated with decreased risk of intermediate and GA AMD but not with CNV. Genetically predicted TG levels were associated with decreased risk of different AMD subtypes. Sensitivity analyses revealed no evidence for directional pleiotropy effects. In our multivariable MR analyses, adjusting for the effects of correlated lipid biomarkers yielded similar results.

Conclusion
These results suggest the role of lipid metabolism in drusen formation and particularly in AMD development at the early and intermediate stages. Mechanistic studies are warranted to investigate the utility of lipid pathways for therapeutic treatment in preventing AMD.
Posttranscriptional modifications in mitochondrial tRNA and its implication in mitochondrial translation and disease
Authors: Kazuhito Tomizawa , Wei Fan-Yan 
Published: August 2020

Abstract
A fundamental aspect of mitochondria is that they possess DNA and protein translation machinery. Mitochondrial DNA encodes 22 tRNAs that translate mitochondrial mRNAs to 13 polypeptides of respiratory complexes. Various chemical modifications have been identified in mitochondrial tRNAs via complex enzymatic processes. A growing body of evidence has demonstrated that these modifications are essential for translation by regulating tRNA stability, structure and mRNA binding, and can be dynamically regulated by the metabolic environment. Importantly, the hypomodification of mitochondrial tRNA due to pathogenic mutations in mitochondrial tRNA genes or nuclear genes encoding modifying enzymes can result in life-threatening mitochondrial diseases in humans. Thus, the mitochondrial tRNA modification is a fundamental mechanism underlying the tight regulation of mitochondrial translation and is essential for life. In this review, we focus on recent findings on the physiological roles of 5-taurinomethyl modification (herein referred as taurine modification) in mitochondrial tRNAs. We summarize the findings in human patients and animal models with a deficiency of taurine modifications and provide pathogenic links to mitochondrial diseases. We anticipate that this review will help understand the complexity of mitochondrial biology and disease.
Where is the tantric mainstream? Discussions with a Bengali Brahmin god-maker in Kolkata
Authors: Bradbury James Samuel 
Published: August 2020

AbstractThe idea of Hindu Tantra as an esoteric, transgressive, or otherwise fringe practice has been revised to account for a ‘tantric mainstream’ in Indian religious life. However, this tantric mainstream has been faintly reflected in the ethnographic record, which would be otherwise well--suited to explore how religious categories are formed in broader social contexts. The religious world of the Bhattacharyas, a Śākta Brahmin family from East Bengal that now lives in suburban Kolkata, evinces some of the challenges in identifying ‘the tantric’ while suggesting alternative framings of ritual practices. Tapan Bhattacharya, a member of the family in his sixties, not only conducted pūjās to the tantric goddess Dakṣiṇā Kālī in their household shrine; he also sculpted the clay statues of deities (pratimā) that were used in local community rituals. Tapan’s nephew, Souvik, drew upon tantric classifications to explain the relations between mainstream household and community pūjās on one hand, and transgressive rituals on the other. Tantra, as understood by members of this household, encompassed a much wider set of relations than conventional binaries of tantra/not-tantra have allowed for. Taking their cue, I locate mainstream Śākta Tantra within the broader contexts of their religious world and the categories that make sense therein, while ultimately recognising that this family’s framings of contemporary religious culture will be matched by other, competing perspectives.
Image-guided endonasal endoscopic excision of Meckel’s cave trigeminal schwannoma from cavernous and petrous carotid artery
Authors: Almomen Ali , Alyousif Amal , Ali Zainab , Al Yaeesh Ibrahim , AlOmirin Ahmed , Al Yahya Abdulwahab , AlSuqair Hesham , Almolani Fadhel 
Published: August 2020

Abstract
Trigeminal schwannoma is the second most common schwannoma after vestibular schwannoma. Symptoms vary depending on the anatomical structures affected by the tumor, including facial pain, paresthesia, dizziness and ataxia. The primary goals are controlling the symptoms and the maintenance of cranial nerves’ integrity perioperatively. We report a 39-year-old lady who was complaining of mild right-sided headache, vision and hearing loss, right facial weakness and dysphagia. CT and MRI showed a large dumbbell-shaped tumor originating from the trigeminal fossa abutting the petrous and cavernous carotid artery and extending to the infratemporal fossa. An image-guided endonasal endoscopic removal was successfully done. Image-guided endonasal endoscopic removal of a trigeminal schwannoma abutting the petrous and cavernous carotid artery and extending to the infratemporal fossa is a safe, effective approach, as it offers excellent visualization, accurate localization and safe dissection of the tumor from the critical anatomical neurovascular structures surrounding it.
Rescuing Vapers Versus Rescuing Smokers: The Ethics
Authors: Eyal Nir 
Published: August 2020

Abstract

Suppose for the sake of argument that wide access to electronic cigarettes would help a great many smokers quit and others avoid smoking, with a dramatic cumulative effect on population health. But suppose also that nowadays, teenagers rarely benefit from that effect, because most do not smoke and are not expected to smoke as adults. Many teenagers do vape. When the net effect of vaping on general public health is expected to remain very positive, should we nevertheless fight vaping, so as to protect teenagers from the direct risks of vaping? For example, what to do if and when certain targeted regulations that would protect teenagers would also, by making it harder to vape, substantially increase smoking in the general population? This situation may be common, and, by pitting the health interests of the general population against those of teenagers, poses an ethical dilemma. This article argues philosophically that if such tradeoffs between the health interests of adolescents and those of the general population exist, morally it is both permissible and preferable to promote the health of the general population.

Implications
In the debate about electronic-cigarette based tobacco harm reduction, some disagreements are factual, for example, disagreements about how harmful vaping is to human lungs, and how much the option of vaping helps smokers quit. However, there is also an ethical debate: if tradeoffs between the health interests of adolescents at risk of vaping and those of the general population (driven by those of smokers) exist, who should be prioritized? This article argues that morally it is sometimes, at least, both permissible and preferable to promote the health of the general population over those of adolescents at risk of vaping.
“Because There’s Just Something About That Menthol”: Exploring African American Smokers’ Perspectives on Menthol Smoking and Local Menthol Sales Restrictions
Authors: D’Silva Joanne , O’Gara Erin , Fryer Craig S , Boyle Raymond G 
Published: August 2020

AbstractIntroductionLocal governments are pursuing policies to limit the availability of menthol cigarettes at the point-of-sale. Although African Americans are disproportionately impacted by menthol cigarettes, little is known about African American smokers’ perspectives on emerging menthol policy. The purpose of this study was to fill a gap in the literature by exploring African American adult (25+) smoker perspectives on menthol and a local menthol sales restriction.MethodsIn-depth semi-structured interviews were conducted with African American smokers (n = 27) in the Minneapolis–St. Paul area June–September 2017. Interviews explored smoking behaviors, harm perceptions, perspectives of menthol in the community and reactions to local menthol sales restrictions. The framework method guided identification of key themes and synthesis of findings.ResultsAlmost all (96%) participants smoked Newport cigarettes. The majority of participants indicated that menthol cigarettes were more harmful than non-menthol cigarettes, citing strength and additives and because they were targeted to African Americans. Some participants were receptive to policy change while others viewed the policy as inconvenient and unfair. Overall, there was a lack of understanding of the policy’s intended public health impact. Some participants indicated that the policy would have no impact on their purchasing or smoking behaviors while others who were contemplating quitting noted that a menthol restriction was encouragement to prompt a quit attempt.ConclusionsSales restrictions can provide a unique opportunity to persuade menthol smokers to quit. Efforts are needed to increase awareness and support of these policies as well as to support African American menthol smokers achieve cessation.ImplicationsThere is growing momentum to restrict local menthol tobacco sales; however, little is known about perceptions among populations most impacted. In Minneapolis–St. Paul, where menthol restrictions were passed in 2017, African American smokers expressed limited awareness and uneven policy support. While some participants were unconvinced the restriction would impact smoking, others indicated it would encourage decreased consumption and prompt quit attempts. There is a need for public education to increase awareness of menthol’s harms, to help menthol smokers quit, and to increase support for menthol policies.
Ecophysiological traits of mixotrophic Strombidium spp
Authors: Maselli Maira , Altenburger Andreas , Stoecker Diane K , Hansen Per Juel 
Published: August 2020

Abstract
Ciliates represent an important trophic link between nanoplankton and mesoplankton. Many species acquire functional chloroplasts from photosynthetic prey, being thus mixotrophs. Little is known about which algae they exploit, and of the relevance of inorganic carbon assimilation to their metabolism. To get insights into these aspects, laboratory cultures of three mixotrophic Strombidium spp. were established and 35 photosynthetic algal species were tested as prey. The relative contributions of ingestion and photosynthesis to total carbon uptake were determined, and responses to prey starvation were studied. Ciliate growth was supported by algal species in the 2–12 μm size range, with cryptophytes and chlorophytes being the best prey types. Inorganic carbon incorporation was only quantitatively important when prey concentration was low (3–100 μgCL−1), when it led to increased gross growth efficiencies. Chla specific inorganic carbon uptake rates were reduced by 60–90% compared to that of the photosynthetic prey. Inorganic carbon uptake alone could not sustain survival of cultures and ciliate populations declined by 25–30% during 5 days of starvation. The results suggest that mixotrophy in Strombidium spp. may substantially bolster the efficiency of trophic transfer when biomass of small primary producers is low.