bluuebunny/crossref_metadata_2025_split huggingface dataset:
A single nucleotide polymorphism assay sheds light on the extent and distribution of genetic diversity, population structure and functional basis of key traits in cultivated north American cannabis
Authors: Henry Philippe , Khatodia Surender , Kapoor Karan , Gonzales Britni , Middleton Alexis , Hong Kevin , Hilyard Aaron , Johnson Steve , Allen Davis , Chester Zachary , Jin Dan , Rodriguez Jule José Carlos , Wilson Iain , Gangola Manu , Broome Jason , Caplan Deron , Adhikary Dinesh , Deyholos Michael K. , Morgan Michael , Hall Oliver W. , Guppy Brent J. , Orser Cindy 
Published: September 2020

Abstract
Background
The taxonomic classification of Cannabis genus has been delineated through three main types: sativa (tall and less branched plant with long and narrow leaves), indica (short and highly branched plant with broader leaves) and ruderalis (heirloom type with short stature, less branching and small thick leaves). While still under discussion, particularly whether the genus is polytypic or monotypic, this broad classification reflects putative geographical origins of each group and putative chemotype and pharmacologic effect.

Methods
Here we describe a thorough investigation of cannabis accessions using a set of 23 highly informative and polymorphic SNP (Single Nucleotide Polymorphism) markers associated with important traits such as cannabinoid and terpenoid expression as well as fibre and resin production. The assay offers insight into cannabis population structure, phylogenetic relationship, population genetics and correlation to secondary metabolite concentrations. We demonstrate the utility of the assay for rapid, repeatable and cost-efficient genotyping of commercial and industrial cannabis accessions for use in product traceability, breeding programs, regulatory compliance and consumer education.

Results
We identified 5 clusters in the sample set, including industrial hemp (K5) and resin hemp, which likely underwent a bottleneck to stabilize cannabidiolic acid (CBDA) accumulation (K2, Type II & III). Tetrahydrocannabinolic acid (THCA) resin (Type I) makes up the other three clusters with terpinolene (K4 - colloquial “sativa” or “Narrow Leaflet Drug” (NLD), myrcene/pinene (K1) and myrcene/limonene/linalool (K3 - colloquial “indica”, “Broad Leaflet Drug” (BLD), which also putatively harbour an active version of the cannabichrometic acid Synthase gene (CBCAS).

Conclusion
The final chemical compositions of cannabis products have key traits related to their genetic identities. Our analyses in the context of the NCBI Cannabis sativa Annotation Release 100 allows for hypothesis testing with regards to secondary metabolite production. Genetic markers related to secondary metabolite production will be important in many sectors of the cannabis marketplace. For example, markers related to THC production will be important for adaptable and compliant large-scale seed production under the new US Domestic Hemp Production Program.
A genome-wide identification of the BLH gene family reveals BLH1 involved in cotton fiber development
Authors: LIU Cuixia , LI Zhifang , DOU Lingling , YUAN Yi , ZOU Changsong , SHANG Haihong , CUI Langjun , XIAO Guanghui 
Published: September 2020

Abstract
Background
Cotton is the world’s largest and most important source of renewable natural fiber. BEL1-like homeodomain (BLH) genes are ubiquitous in plants and have been reported to contribute to plant development. However, there is no comprehensive characterization of this gene family in cotton. In this study, 32, 16, and 18 BLH genes were identified from the G. hirsutum, G. arboreum, and G. raimondii genome, respectively. In addition, we also studied the phylogenetic relationships, chromosomal location, gene structure, and gene expression patterns of the BLH genes.

Results
The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic analysis. Among them, 25 members were assigned to 15 chromosomes. Furthermore, gene structure, chromosomal location, conserved motifs, and expression level of BLH genes were investigated in G. hirsutum. Expression profiles analysis showed that four genes (GhBLH1_3, GhBLH1_4, GhBLH1_5, and GhBLH1_6) from BLH1 subfamily were highly expressed during the fiber cell elongation period. The expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid, but not auxin. Exogenous application of gibberellic acid significantly enhanced GhBLH1_3, GhBLH1_4, and GhBLH1_5 transcripts. Expression levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid treatment.

Conclusions
The BLH gene family plays a very important role in many biological processes during plant growth and development. This study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.
Spatiotemporal perspectives on urban energy transitions: a comparative study of three cities in China
Authors: Castán Broto Vanesa , Mah Daphne , Zhang Fangzhu , Huang Ping , Lo Kevin , Westman Linda 
Published: September 2020

AbstractThis paper develops an integrated framework to study the socio-spatial and temporal dimensions of urban energy transitions to investigate the development and spread of solar energy technologies in urban China. A comparative analysis of three case studies of solar energy transitions in the cities of Foshan (in Guangdong), Rizhao (in Shandong), and Wuxi (in Jiangsu) demonstrates the framework’s applicability. The results map each city’s trajectory towards low carbon energy. Transitions result from dynamic interactions among central and local governments, solar manufacturers, solar installers, and residents. Alongside industrial strategies, locally-specific factors have a determining influence on the eventual outcomes.
The role of neurohormonal blockers in the primary prevention of acute-, early-, and late-onset anthracycline-induced cardiotoxicity
Authors: Ayuna Ahmed , Abidin Nik 
Published: September 2020

Abstract
Background
Anthracycline-induced cardiotoxicity has been classified based on its onset into acute, early, and late. It may have a significant burden on the quality and quantity of life of those exposed to this class of medication. Currently, there are several ongoing debates on the role of different measures in the primary prevention of cardiotoxicity in cancer survivors. Our article aims to focus on the role of neurohormonal blockers in the primary prevention of anthracycline-induced cardiotoxicity, whether it is acute, early, or late onset.

Main body of the abstract
PubMed and Google Scholar database were searched for the relevant articles; we reviewed and appraised 15 RCTs, and we found that angiotensin-converting enzyme inhibitors (ACEI) and B-blockers were the most commonly used agents. Angiotensin II receptor blockers (ARBs) and mineralocorticoid receptor antagonists (MRAs) were used in a few other trials. The follow-up period was on the range of 1–156 weeks (mode 26 weeks). Left ventricular ejection fraction (LVEF), left ventricular diameters, and diastolic function were assessed by either echocardiogram or occasionally by cardiac magnetic resonance imaging (MRI). The occurrence of myocardial injury was assessed by troponin I. It was obvious that neurohormonal blockers reduced the occurrence of LVEF and myocardial injury in 14/15 RCTs.

Short conclusion
Beta-blockers, especially carvedilol and ACEI, especially enalapril, should be considered for the primary prevention of acute- and early-onset cardiotoxicity. ARB and MRA are suitable alternatives when patients are intolerant to ACE-I and B-blockers. We recommend further studies to explore and establish the role of neurohormonal blockers in the primary prevention of the acute-, early-, and late-onset cardiotoxicity.
Uniportal video-assisted thoracoscopic drainless sympathectomy for palmar and palmo-plantar hyperhidrosis: an institutional experience
Authors: El-Hag-Aly Mohammed Ahmed , Soliman Rafik Fekry , Zaid Nehad Abdo , Eldesouky Mahmoud Said , Bakoush Mohamed Mordy , Hagag Mohamed Gamal 
Published: September 2020

Abstract
Background
Primary hyperhidrosis (PH) still impacts negatively the patient’s quality of life (QoL). Progressively, it leads to poorer QoL regardless of gender. The endoscopic thoracic sympathectomy has been used safely and effectively for control of palmar hyperhidrosis, but it is still questionable in palmo-plantar type. We assessed the benefits of uniportal drainless thoracoscopic sympathectomy (UDTS) for palmo-plantar hyperhidrosis patients compared to palmar ones.
This prospective study comprised 213 consecutive patients with PH. They underwent bilateral simultaneous UDTS. We used the Hyperhidrosis Disease Severity Scale (HDSS) scoring system for assessment of QoL pre- and postoperatively. Follow-up was continued for 1-year that was aiming to detect the QoL improvement, recurrence, and compensatory hyperhidrosis (CH).

Results
All patients experienced immediate complete resolution of hyperhidrosis postoperatively. Recurrence was encountered in one patient during the first 6 months. All patients had improved QoL postoperatively, but at the end of 1-year follow-up, 2.8% of patients were still suffering moderate to severe impaired QoL. Mean hospital stay was 31.79 ± 17.5 h. We found significant longer hospital stay in palmar group than palmo-plantar group. There was no significant difference between both groups in neither operative time, recurrence, nor CH.

Conclusions
UDTS offers better QoL for patients with PH even the palmo-plantar type. The palmo-plantar type benefits similarly to the palmar type in terms of comparable postoperative QoL and CH incidence.
Establishing the pattern of the vertebrate limb
Authors: McQueen Caitlin , Towers Matthew 
Published: September 2020

ABSTRACT
The vertebrate limb continues to serve as an influential model of growth, morphogenesis and pattern formation. With this Review, we aim to give an up-to-date picture of how a population of undifferentiated cells develops into the complex pattern of the limb. Focussing largely on mouse and chick studies, we concentrate on the positioning of the limbs, the formation of the limb bud, the establishment of the principal limb axes, the specification of pattern, the integration of pattern formation with growth and the determination of digit number. We also discuss the important, but little understood, topic of how gene expression is interpreted into morphology.
Intrafractional fiducial marker position variations in stereotactic liver radiotherapy during voluntary deep inspiration breath-hold
Authors: Stick Line Bjerregaard , Vogelius Ivan Richter , Risum Signe , Josipovic Mirjana 
Published: September 2020

Objectives: To evaluate intrafractional fiducial marker position variations during stereotactic body radiotherapy (SBRT) in patients treated for liver metastases in visually guided, voluntary deep inspiration breath-hold (DIBH). Methods: 10 patients with implanted fiducial markers were studied. Respiratory coaching with visual guidance was used to ensure comfortable voluntary breath-holds for SBRT imaging and delivery. Three DIBH CTs were acquired for treatment planning. Pre- and post-treatment CBCTs were acquired for each of the three treatment fractions. Per-fraction marker position was evaluated on planar 2D kV images acquired during treatment fractions for 4 of the 10 patients. Results: The median difference in marker position was 0.3 cm (range, 0.0–0.9 cm) between the three DIBH CTs and 0.3 cm (range, 0.1 to 1.4 cm) between pre- and post-treatment CBCTs. The maximum intrafractional variation in marker position in craniocaudal (CC) direction on planar kV images was 0.7 to 1.3 cm and up to 1.0 cm during a single DIBH. Conclusion: Difference in marker position of up to 1.0 cm was observed during a single DIBH despite use of narrow external gating window and visual feedback. Stability examination on pre-treatment DIBH CTs was not sufficient to guarantee per-fraction stability. Evaluation of differences in marker position on pre- and post-treatment CBCT did not always reveal the full magnitude of the intrafractional variation. Advances in knowledge To increase treatment accuracy, it is necessary to apply real-time monitoring of the tumour or a reliable internal surrogate when delivering liver SBRT in voluntary DIBH.
ACE inhibitor, captopril, attenuates cytopathic effects of herpes simplex virus 1 in SH-SY5Y cells
Authors: Wofford Amina S. , Standiford Adam , Harris Kennedy , Salam Ahmad B. , Jackson Cynthia A. , Bradford Chastity N. , Griffin Gerald D. 
Published: September 2020

Background: Over 60% of the United States population is infected with herpes simplex virus 1 (HSV-1). Current HSV-1 treatment regimens exert their antiviral effects through a common mechanism of action and suffer from high dosing frequencies, which may contribute to patient noncompliance and the subsequent development of antiviral resistance. Although primarily known for their functions in maintaining homeostatic control of arterial blood and osmotic pressures, components of the Renin-Angiotensin Aldosterone System (RAAS) have been implicated in viral replication and some components demonstrated antiviral properties. However, the antiviral properties of RAAS components have not been well characterized in reference to HSV-1. Methods: To address this gap in knowledge, we evaluated the antiviral effects of captopril, an Angiotensin Converting Enzyme 1 (ACE-1) inhibitor, on HSV-1 infection in SH-SY5Y neuroblastoma cells. We demonstrated that captopril attenuates HSV-1-induced cytopathic effects (CPE) via cell-based and morphological assays. To investigate the potential mechanism, we conducted molecular modeling studies and identified the ability of captopril to interact with and bind HSV-1 glycoprotein D. To determine where in the virus life cycle captopril exerts its protective effects, we performed experiments observing the effect of captopril on both viral entry and replication utilizing a green fluorescent protein-tagged virus and subsequent quantitative Polymerase Chain Reaction. Results: Results suggest captopril protects cells from HSV-1-induced CPE through its effect on viral replication by increasing cell viability in infected cells and decreasing virus replication, which we propose is modulated through the decreased expression of ICP0. Conclusions: Collectively, the results presented here support further evaluation of captopril, and other RAAS components, as a basis for potential novel therapeutic interventions for the treatment of HSV-1, its associated pathologies, and potentially other virus infections.
Assessing the impact of tobacco control policies on smokeless tobacco uptake and use among secondary school students in South Asia: protocol for a feasibility study of conducting longitudinal surveys
Authors: Mishu Masuma Pervin , Siddiqi Kamran , McNeill Ann , Kanaan Mona , Jackson Cath , Huque Rumana , Kanan Sushama , Abdullah S.M. , Fieroze Fariza , Garg Suneela , Singh M. Meghachandra , Borle Amod L. , Deshmukh Chetana , Akhter Zohaib , Mazhar Laraib , Khan Zohaib , Rehman Khalid , Ullah Safat , Han Lu , Readshaw Anne , Iqbal Romaina 
Published: September 2020

Background: Smokeless tobacco (ST) use is common among youth in South Asia where 85% of the world’s 300 million ST users live and use the most lethal ST forms. Little is known about the impact of tobacco control policies on the youth ST uptake in those countries. We planned to conduct longitudinal surveys among secondary school students to evaluate existing tobacco control policies on ST uptake and use, and a feasibility study for that prospective, observational cohort study. Study objectives: (1) To demonstrate the feasibility of selection, recruitment and retention of schools and of study participants; (2) To assess the feasibility and acceptability of the study procedure and study tool (questionnaire); (3) To assess if the questionnaire can assess tobacco uptake and use, and the potential predictors of tobacco uptake and use of the envisaged main study. Methods and analysis: The feasibility study will be conducted in two administrative areas within each of three South Asian countries: Bangladesh, India and Pakistan. We will use both quantitative and qualitative data collection methods. Eight eligible secondary schools will be randomly selected within purposively selected sub-districts from each country. We plan to conduct one baseline and one follow up survey among secondary school students, one year apart. At each time point, data on tobacco uptake and the potential predictors will be collected from students via self-administered questionnaires. The qualitative component will be embedded into the study with each round of data collection to assess the acceptability of the study instrument (questionnaire) and data collection methods, via focus group discussions with students and semi-structured interviews with schoolteachers. Recruitment and retention rates, completeness of the questionnaires, frequencies and associations of tobacco use and explanatory variables will be reported. Data gathered from the focus group and interviews will be analysed using the framework approach.
On the use of real-time reported mortality data in modelling and analysis during an epidemic outbreak
Authors: Liljenberg Per 
Published: September 2020

Background: For diseases like Covid-19, where it has been difficult to identify the true number of infected people, or where the number of known cases is heavily influenced by the number of tests performed, hospitalizations and deaths play a significant role in understanding the epidemic and in determining the appropriate response. However, the Covid-19 deaths data reported by some countries display a significant weekly variability, which can make the interpretation and use of the death data in analysis and modeling difficult. Methods: We derive the mathematical relationship between the series of new daily deaths by reporting date and the series of deaths by death date. We then apply this formalism to the corresponding time-series reported by Sweden during the Covid-19 pandemic. Results: The practice of reporting new deaths daily, as is standard procedure during an outbreak in most countries and regions, should be viewed as a time-dependent filter, modulating the underlying true death curve. After having characterized the Swedish reporting process, we show how smoothing of the Swedish reported daily deaths series results in a curve distinctly different from the true death curve. We also comment on the use of nowcasting methods. Conclusions: Modelers and analysts using the series of new daily deaths by reporting date should take extra care when it is highly variable and when there is a significant reporting delay. It might be appropriate to instead use the series of deaths by death date combined with a nowcasting algorithm as basis for their analysis.
Housing with support for older people: a mixed-methods systematic review protocol
Authors: Coyle Camille , Buggy Sarah , Cagney Olivia , Farragher Louise , Lee Caitriona , Patje Darren , Long Jean 
Published: September 2020

Background: The implementation of housing with support is rapidly expanding, particularly as life expectancy is increasing throughout the world. This expansion is likely to intensify in the context of coronavirus disease 2019 (COVID-19), which has revealed the risks of relying primarily on nursing homes. This mixed-methods systematic review aims to: 1) explore older people’s perceptions and experiences of housing with support and 2) examine the impact of providing housing with support for older people. Methods: The databases Ovid Medline, Ovid Social Policy & Practice, EBSCO CINAHL, and EBSCO SOCIndex will be searched, and grey literature will also be identified. Quality assessment will be carried out using Joanna Briggs Institute’s Critical Appraisal Checklist for Qualitative Research as well as a tool from the National Institutes of Health for observational cohort studies. This review will employ convergent parallel design; as such, qualitative and quantitative findings will be synthesised separately in the initial stage of analysis. The results from the qualitative and quantitative syntheses will then be integrated in the final stage of the analysis. Conclusion: This systematic review will synthesise the evidence regarding older people’s perceptions and experiences of housing with support and the impact of providing housing with support for older people on their quality of life.
Implementing a National Approach to Research Ethics Review during a Pandemic – the Irish Experience
Authors: Sheehy Aileen , Ralph James Jennifer , Horgan Mary 
Published: September 2020

The surge of coronavirus disease 2019 (COVID-19) research studies involving human participants in response to the pandemic has meant that research ethics committees across the world have been challenged to adapt their processes to meet demand while retaining high standards of review. Ethics review during this pandemic remains essential to ensure the safety, dignity and well-being of research participants, however research ethics committees are now faced with new, and often complex, ethics considerations and logistical challenges. This Open Letter looks specifically at the Irish experience of establishing a national approach to research ethics review amidst a global pandemic. This represents Ireland’s first National Research Ethics Committee, which provided the research community with an expedited and ‘single national opinion’ for ethics review for COVID-related research. The insights gleaned and lessons learned from the Irish experience may inform emergency responses to future pandemics or public health emergencies.
All-metallic metasurfaces towards high-performance magneto-plasmonic sensing devices
Authors: Li Lixia , Zong Xueyang , Liu Yufang 
Published: September 2020

Magneto-plasmonic sensors based on surface plasmon resonance have been studied considerably in recent years, as they feature high sensitivity and ultrahigh resolution. However, the majority of such investigations focus on prism-based sandwich architectures that not only impede the miniaturization of devices but also have a weak transverse magneto-optical Kerr effect (TMOKE) in magnitude. Herein, we theoretically demonstrate a magneto-plasmonic sensor composed of Au/Co bilayer nanodisk arrays on top of optically thick metallic films, which supports a narrow surface plasmon resonance (SPR) with a bandwidth of 7 nm and allows for refractive index sensitivities as high as 717 nm/RIU. Thanks to the high-quality SPR mode, a Fano-like TMOKE spectrum with a subnanometer bandwidth can be achieved in the proposed structure, thereby giving rise to ultrahigh sensing of merit values as large as 7000 in water. Moreover, we demonstrate a large TMOKE magnitude that exceeds 0.6. The value is 1 order of magnitude larger than that of magneto-plasmonic sensors reported. We also demonstrate that the behavior of TMOKE spectra can be controlled by tuning the geometrical parameters of the device including the diameter and thickness of nanodisk arrays. This work provides a promising route for designing magneto-plasmonic sensors based on metasurfaces or metamaterials.