bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shifting the use of Leuit in agricultural systems
Authors: Fathurohmah Ani , Prasetyo Djoko Adi 
Published: November 2020

Leuit is a grain bin and has a function to store the community’s rice grains of Suka Puyuh Village. This research aims to describe the preservation effort of Leuit in Suka Puyuh Village. Leuit has been preserved through family trait, Leuit reactivation program, and exploiting ruwat lembur tradition as the mechanism. The villagers’ attempts to preserve the Leuit intrigued the researcher to conduct further research concerning the existence of the Leuit in Suka Puyuh Village. This research is an ethnographic research along with employing a descriptive method and qualitative approach. Further, the technique of data collection uses observation methods and depth interviews. The obtained data are analyzed using Wilbert E. Moore’s Social Change theory. The results of this study indicate that Leuit in Kampung Suka Puyu’s community life has undergone a shift because of the change of social structure. Development of the agricultural system from simple to a more advanced and varied level has an impact on the shift of farming system to gardening system.
Further Optimization of VFD Controllers
Authors: Hinojosa Tim , Huckaby Chance , Cavazos Chris 
Published: November 2020

Abstract
Variable Frequency Drives (VFDs) are an advanced type of beam pump-off controller with the ability to increase or decrease pumping speed. This facilitates drawing down the fluid level to near pump off conditions and maintaining a constant bottom hole pressure, all while running 24 hours per day. VFDs have been successfully utilized over the last few years to reduce the failure frequency in the most problematic wells, and in some cases, increase production as well.
Installation of A Sucker Rod Pumping System Over a Failed Electrical Submersible Pumping System to Recover Production Using Rigless Intervention
Authors: De Lima Fábio Soares , De Souza Carlos Francisco , Neto José Paulino 
Published: November 2020

Abstract
This paper explains the design and the execution of a rigless intervention to resume production from a dry completion offshore installing successfully a Sucker Rod Pump (SRP) system replace a faulted redundant Electrical Submersible Pumping (ESP) system.
Nowadays all oil companies need to work with small budgets and must search for methods of well intervention that reduce operational costs. Considering an offshore field, any intervention is very expensive. In the present case, in a unique well located in caisson platform, a conventional workover would consist recovering and replacing ESP system set at 3.350 ft that operated for about 10 years. To change the bottom hole equipment, a jackup rig was needed, but such a ring was not available and, even if available, its cost probably would be prohibitive for production maintenance. The solution adopted was to run SRP system with an insert pump anchor enabling it to be set at any depth inside the tubing, above the failed ESP assembly. A Linear Rod Pumping (LRP®) was chosen because its profile and its small footprint that allowed its installation with the small Caisson Platform topside area. A structure ("portico") was built on the caisson to perform the new system installation. The successful use of the SRP system permitted to resume production with short well downtime and reduced intervention costs, eliminating the need for a the workover jackup rig.
Even though the outcome of the installed SRP system is around 50% of the last ESP registered flow rate, the solution was an economically attractive option to keep production and also allows future changes of the lifting system without the need of a workover rig.
Investigation of an anthrax outbreak in Makoni District, Zimbabwe
Authors: Makurumidze Richard , Tafara Gombe Notion , Tapuwa Magure , Tshimanga Mufuta 
Published: November 2020

Abstract
Background: Anthrax continues to be a disease of public health importance in Zimbabwe, with sporadic outbreaks reported annually in many parts of the country. A human anthrax outbreak occurred in wards 22 and 23 of Makoni District from mid-June 2013 to end of January 2014, following cattle deaths in the wards. Laboratory tests confirmed anthrax as the cause for the cattle deaths. This study aims to investigate the clinical characteristics, distribution of anthrax cases (places, person and time), risk factors for contracting the disease, environmental assessment, district preparedness and response, and outbreak prevention and control measures.Methods: We conducted an outbreak investigation using a mixed-methods design. A 1:1 case-control study was used to assess risk factors for contracting anthrax. The controls were frequency matched to cases by sex. Data were collected using a structured interviewer-administered questionnaire. Environmental assessment, district preparedness and response, and outbreak prevention and control measures were assessed using a checklist, observations, and key informant interviews. Multivariable unconditional logic regression analysis was performed to identify independent risk factors associated with contracting anthrax.Results: We interviewed 37 of the 64 cases, along with 37 controls. All the cases had cutaneous anthrax, with the hand being the most common site of the eschar (43%). Most of the cases (89%) were managed according to the national guidelines. Multivariable analysis demonstrated that meat sourced from other villages [vs butchery, OR = 15.21, 95% CI (2.32-99.81)], skinning [OR = 4.32, 95% CI (1.25-14.94)], and belonging to religions that permit eating meat from cattle killed due to unknown causes or butchered after unobserved death [OR = 6.12, 95% CI (1.28-29.37)] were associated with contracting anthrax. The poor availability of resources in the district caused a delayed response to the outbreak.Conclusion: The described anthrax outbreak was caused due to contact with infected cattle meat. Although the outbreak was eventually controlled through cattle vaccination and health education and awareness campaigns, the response of the district office was initially delayed and insufficient. The district should strengthen its emergency preparedness and response capacity, revive zoonotic committees, conduct awareness campaigns and improve surveillance, especially during outbreak seasons.
Parental drinking according to parental composition and adolescent binge drinking: Findings from a nationwide high school survey in Japan
Authors: Inoura Satoshi , Shimane Takuya , Kitagaki Kunihiko , Wada Kiyoshi , Matsumoto Toshihiko 
Published: November 2020

Abstract
Background: Alcohol problems in parents have been revealed to affect adolescent alcohol misuse. However, few studies examine the effects of parental drinking on adolescent risky drinking (including binge drinking) in the general population. In particular, previous study findings are inconsistent regarding the influence of parental drinking according to parental composition. In this study, we aimed to examine the relationship between parental drinking, according to parental composition, and binge drinking among high school students in Japan.Methods: We performed a secondary analysis of the Nationwide High School Survey on Drug Use and Lifestyle 2018, Japan. A total of 46,848 valid surveys from high school students of 78 schools were included for analysis. Logistic regression analysis with a generalized linear mixed model was conducted with binge drinking as the dependent variable and “parental drinking according to parental composition” (e.g., father’s drinking, mother’s drinking, father’s absence, mother’s absence, both parents drinking, and neither parent at home) as the independent variable, after adjusting with covariates. Binge drinking was defined as five or more alcoholic drinks for male adolescents or four or more alcoholic drinks for females on the same occasion within two hours. Results: In the fully adjusted models, adolescents whose mothers drink (adjusted odds ratio (AOR): 1.50, 95% confidence interval (CI): 1.06–2.12) were significantly associated with adolescent binge drinking. This risk was significantly higher among students with neither parent living at home (AOR: 4.35, 95% CI: 2.10–9.02). Conclusion: Parental drinking and absence do affect adolescent binge drinking; our findings show that adolescents are more likely to engage in binge drinking if their mothers drink or if they are not living with either parent. Therefore, it is important to engage parents and non-parental family members in future programs and interventions to prevent adolescent binge drinking.
Diversity and Prevalence of Antibiotic Resistance Genes, Virulence Factors, and the Microbiome in Aquaculture in Southern China Revealed by Metagenomic Sequencing
Authors: Su Haochang , Xu Wujie , Hu Xiaojuan , Xu Yu , Wen Guoliang , Cao Yucheng 
Published: November 2020

Abstract
Background: Microbiota carrying multiple antibiotic resistance genes (ARGs) and virulence factors (VFs) are posing increasing risks to public health. Particularly the rapid spread of human pathogenic bacteria (HPB) with antibiotic resistance is recognized as a top health issue. The occurrence and abundance of ARGs in aquaculture have been investigated following metagenomic approaches. However, few studies have investigated the antibiotic resistome and VFs and their HPB hosts in aquaculture. Moreover, the relationships between ARGs and VFs and their microbiome in aquaculture are poorly understood. Results: The profiles of the antibiotic resistome, VFs, and HPB in aquaculture in Southern China were investigated. In total, 492 subtypes of 24 ARGs types were detected. Multidrug ARGs were most predominant, followed by macrolide-lincosamide-streptogramin (MLS). Proteobacteria were the most predominant phylum carrying ARGs, followed by Firmicutes. Fifty-two HPB genera were detected. Firmicutes was the most abundant phylum, followed by Proteobacteria. Staphylococcus was the most abundant HPB genus. The samples contained 363 VFs, with Capsule being the most abundant. Seven HPB phyla, including 42 HPB genera, carried VFs, and the abundance of Bacillus was highest. The abundances of ARGs and VFs were highest in the sediment. However, the abundance of HPB was highest in shrimp guts and Staphylococcus was most abundant. Most ARGs were more prevalent on chromosomes than on plasmids. Source tracking analysis showed that the sediment was the greatest contributor to microbes carrying ARGs, VFs, and HPB in shrimp guts. Additionally, the water source contributed some of the HPB of shrimp guts. Conclusions: This study provides in-depth profiles of the abundances, diversity, distribution, and prevalence of ARGs, VFs, and their hosts HPB in aquaculture for the first time. Sediment was the most direct and important contributor to the ARGs, VFs, and HPB in the shrimp guts. The prevalence of HPB in aquaculture, particularly the high abundance of Staphylococcus in shrimp guts, poses potential risks to human health and food safety. Aquaculture water sources should be monitored and protected. The findings of this study provide a better understanding of the dissemination and hosts of ARGs and VFs for improving aquaculture management and public health surveillance.
Developing a Conceptual Model to Understand the&nbsp;Nutritional Problems and Negative Impacts on Quality of Life among Chinese Patients with type 2 Diabetes: A Qualitative Study
Authors: wang yun , yuan xiangping , gu xianju , meng changxiu , zheng yanling 
Published: November 2020

Abstract
BackgroundGreat challenges have been reported in China with respect to the largest number of patients with type 2 diabetes mellitus and the poor nutrition management. It was generally agreed that healthy diet and regular physical activity could promote the management of diabetes, with emerging research indicating potential delay and prevention of T2DM and its complications. However, currently no conceptual model has been proposed regarding the nutritional problems and its relationship with quality of life among Chinese patients with type 2 diabetes. This study adopted a qualitative method in order to obtain a firsthand and an in-depth understanding about the nutritional problems and concerns among patients with type 2 diabetes, and explored the negative impacts of insufficient nutrition management on quality of life.MethodsTwenty-one adult patients with type 2 diabetes from five hospitals in the southwest of China participated in a semi-structured interview regarding the nutritional problems and negative impacts on quality of life among Chinese patients with type 2 diabetes. Recordings were transcribed verbatim and content analysis were adopted to identify themes. ResultsFive themes emerged from the analysis to represent the nutritional problems and negative impacts on quality of life among Chinese patients with type 2 diabetes: knowledge about nutrition, social aspects of nutrition, cultural aspects of nutrition, behaviors and nutrition management, and health outcomes of nutrition.ConclusionsNutrition issues and its negative impacts on quality of life need to be considered in nursing care among Chinese patients with type 2 diabetes. Personal, social, cultural and religious factors should all be taken into consideration when promoting nutrition knowledge and behaviors.
Risk Factors for Development of High-Risk Stigmata in Branch-Duct Intraductal Papillary Mucinous Neoplasms
Authors: Yamazaki Tatasuhiro , Tomoda Takeshi , Kato Hironari , Miyamoto Kazuyuki , Matsumi Akihiro , Ueta Eijiro , Fujii Yuki , Saragai Yosuke , Uchida Daisuke , Matsumoto Kazuyuki , Horiguchi Shigeru , Tsutsumi Koichiro , Okada Hiroyuki 
Published: November 2020

Abstract
Background: Strict follow-up is recommended for branch-duct intraductal papillary mucinous neoplasms (BD-IPMN) to avoid missing the development of high-risk stigmata (HRS) at a premalignant stage. This study aimed to identify the risk factors associated with the development of HRS during follow-up.Methods: We performed a retrospective analysis of 283 patients with BD-IPMN, treated at the Okayama University Hospital in Japan between January 2009 and December 2016. Only patients with imaging studies indicative of classical features of BD-IPMN without HRS and followed for >1 year were included in the study. We performed radiological follow-up every 6 months and collected patients’ demographic data, cyst characteristics, and clinical outcomes. Cyst size was recorded at the initial and final imaging studies and growth rate was calculated. The primary outcome was to evaluate the risk factors for development of HRS in patients with BD-IPMN without HRS at the initial diagnosis.Results: Patients with BD-IPMN had a median follow-up of 53.3 months. Based on image analyses, a mean cysts size were initially 18.0 mm and their final size was 20.4 mm, and the mean annual cyst growth was 0.57 mm. Among them, 10 patients (3.5%) developed HRS after a median surveillance period of 55.8 months. Main pancreatic duct (MPD) size (5-9 mm) and cyst growth rate (>2.5 mm/year) were, both, independent risk factors for the development of HRS (odds ratio, 14.2; 95% CI, 3.1-65.2, P = .0006, and odds ratio, 6.1; 95% CI 1.5-25.5, P = .014). Considering the number of worrisome features (WFs), the rate of HRS development was not a WF: 2.0% (4/199), a single WF: 1.6% (1/62), multiple WFs: 22.7% (5/22), and significantly higher in multiple WFs (95% CI: 4.0–57.1; p=.0003).Conclusions: MPD dilation, rapid cyst growth, and multiple WFs were significant risk factors for the development of HRS. In the presence of such features, it is necessary to closely follow the development of HRS and avoid missing the best opportunity for surgical intervention.
Trajectory of Inflammatory Biomarkers to Predict Intra-Abdominal Septic Complications After Surgery for Crohn’s Disease
Authors: Yu Zeqian , Feng Dengyu , Wang Zhen , Xu Yi , Zhao Lei , Yi Xianqi , Gong Jianfeng 
Published: November 2020

Abstract
Background: Postoperative infectious complications, especially intra-abdominal septic complications (IASCs), represent a major cause of morbidity after surgery for Crohn's disease (CD). We aimed to assess the association between postoperative trajectory of C-reactive protein (CRP), interleukin-6 (IL-6) and procalcitonin (PCT) and postoperative IASCs of CD bowel surgery.Methods: This is a single-center, prospective, observational study between July 2016 and November 2018, 202 consecutive adults underwent CD-related intestinal resection and anastomosis were enrolled. Levels of CRP, IL-6 and PCT were measured preoperatively and for five days postoperatively. Postoperative IASCs were recorded accordingly.Results: Postoperative IASCs were detected in 24 (11.76%) patients. For prediction of isolated timepoints, IL-6 has higher area under curve (AUC) compared to CRP and PCT in predicting postoperative IASCs (AUC: 0.85 on POD 1; 0.87 on POD 3 and 0.90 on POD 5). For trend analysis, an CRP increase of more than 50 mg/L from POD 3 to POD 5 had a highest AUC of 0.80. An average CRP on POD 3 and POD 5 of >100.3 mg/L had an AUC of 0.947. Conclusions: CRP and IL-6 outperforms PCT in predicting postoperative IASCs in terms of trajectory and cutoff values. Continued elevation of CRP showed excellent performance in diagnosing IASCs.
Whole-genome epigenetic function annotation through sgRNA libraries synthesized by controlled template-dependent elongation
Authors: Jia Junling , Pan Chen , Li Ran , Shui Liyan , Wang Yali , Xiao Zhengyun , Zhu Jing , Wu Chao , Zheng Min 
Published: November 2020

Abstract
Epigenome is the set of DNA-associated proteins or chemical modifications to DNA, which regulates gene expression in processes of development and disease. While current advances have allowed researchers to routinely profile epigenomes from given samples, our understandings of the functions of epigenetic hallmarks are nonspecific at best. Applying CRISPR-screening to genome-widely interrogate the function of individual epigenetic hallmarks demands massive sgRNA libraries which are unaffordable via commercial syntheses. Our development consists of a high throughput and cost-effective controlled template-dependent elongation (CTDE) approach which converts source DNA to sgRNA templates. Affiliated screenings encompass 3.8M sgRNAs generated by CTDE targeting all major H3K4me3 and CTCF hallmarks in mESCs and HepG2 and identified 20K essential epigenetic hallmarks, which render the first batch of functional epigenome annotation of H3K4me3 and CTCF hallmarks in mammals. As an application example, we show that a H3K4me3 hallmark orchestrates CDC42 level and cell-cycle progression through promoting LINC00339 expression in HepG2.
Clinical Features and Risk Factors for Secondary Infection in Critically Ill Patients With COVID-19
Authors: Pan Tongtong , Chen Dazhi , Chen Yi , Pan Chenwei , Su Feifei , Hong Liang , Li Huili , Zhao Hui , Lin Zhuo , Wang Xiaodong , Lin Hongwei , Cai Chao , Chen Yong-Ping 
Published: November 2020

Abstract
Background: To analyze the clinical features and the possible risk factors of secondary infection, and explore their impact on prognosis of COVID-19. Methods: A total of 165 severe and critical hospitalized patients diagnosed with COVID-19 were included. The clinical characteristics, laboratory tests, imaging data, secondary infections and outcomes were analyzed. Results: The mean age of total patients was (57.3±15.2) years, of which 111 were males (67.3%). 108 cases were with basic diseases (65.5%), and 1 death (0.6%). The secondary infection rate in critical patients was significantly higher than in severe patients (P <0.05). The secondary infections were mainly lung infections. The pathogens were principally Burkholderia multivorans, Stenotrophomonas maltophilia, Acinetobacter baumannii and Klebsiella pneumoniae. The recovery rate of 28 days in the infected group was significantly lower than that in the non-infected group (p < 0.001).The utilization rate and usage time of invasive ventilator, and deep vein catheterization, catheter indwelling and ECMO were the risk factors for the secondary infected patients.Conclusion: Secondary infection is an extremely common complication in critically ill patients and a trigger point for exacerbation of the disease. An effective control on the secondary infection will do good to the prognosis of COVID-19 patients.
Identification Candidate Diagnostic Biomarkers Between Minimal Change Disease and Focal Segmental Glomerulosclerosis by Bioinformatics Analysis
Authors: Jiang Xue , Chen Laite , Du Yuanyuan , Li Yayu 
Published: November 2020

Abstract
Background: Minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) are common causes of nephrotic syndrome which have similar clinical as well as histologic magnification and hard to differentiate. This study aimed to identify novel biomarkers to distinguish FSGS and MCD through bioinformatics analysis and elucidate the possible molecular mechanism.Methods: Based on the microarray datasets GSE104948 and GSE108113 downloaded from the Gene Expression Omnibus database, the differentially expressed genes (DEGs) between FSGS vs healthy control , MCD vs healthy control were identified, and further defined by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Hub genes were checked by protein-protein interaction networks.Results: A total of and 358 and 368 genes were identified in FSGS and MCD compared with healthy controls, among them, there were156 overlapping DEGs. GO analysis showed the DEGs in these two diseases were simultaneously enriched in mRNA splicing, RNA polymerase II transcription, mRNA export, insulin stimulus, integrin-mediated signaling pathway, viral process and phagocytosis. Module analysis showed that genes in the top 1 significant module of the PPI network were mainly associated with Spliceosome among FSGS and MCD. The top 10 hub genes analysis discovered that Most of hub genes were same between two disease, while among these genes, CD2 cytoplasmic tail binding protein 2 (CD2BP2), U6 snRNA-associated Sm-like protein (LSM8) and Small nuclear ribonucleoprotein polypeptides B (SNRPB) only differential expression in FSGS and Splicing factor 3A, subunit 3 (SF3A3) only differential expression in MCD, which may be used for differential diagnosis of these two diseases in the future.Conclusions: We identified key genes and mainly pathway associated with FSGS and MCD. Our results provide a set of potential genes used for differential diagnosis of these two diseases.
METTL13 Mediates the Translation of Snail in Head and Neck Squamous Cell Carcinoma
Authors: Wang Xiaochen , Li Kang , Wan Yuehan , Cheng Maosheng , Xiong Gan , Wang Ganping , Chen Shuang , Chen Zhi , Chen Jianwen , Xu Xiuyun , Wang Cheng , Peng Liang , Chen Demeng 
Published: November 2020

Abstract
Background: Accumulating evidence supports that Methyltransferase like 13 (METTL13), which is implicated in protein binding and synthesis, act as oncogenic molecule. This study aimed to reveal the potential mechanism of METTL13 in head and neck squamous cell carcinoma (HNSCC) via mediating enhancing translation efficiency of Snail. Methods: The expression of METTL13, Snail and ki67 were detected in patient HNSCC and paired para-carcinoma tissues. Knockdown and ectopic expression experiments were used in HNSCC cells to investigate their effects on tumor cell migration, proliferation and apoptosis. RNA-sequencing, polysome profiling and SUnSET assays were developed to obtain the further mechanism of the interaction among METTL13, Snail and translation.Results: METTL13 was signiﬁcantly upregulated in HNSCC at both mRNA and protein level. Increased METTL13 was negatively associated with clinical prognosis. And METTL13 markedly affected HNSCC cellular phenotypes in vivo and vitro. Further mechanism study revealed that METTL13 could regulate EMT signaling pathway by mediating enhancing translation efficiency of Snail, the key transcription factor in EMT, hence regulating the progression of EMT. Conclusion: Altogether, our study had revealed the clinical signiﬁcance of METTL13 in HNSCC, and providing a potential therapeutic strategy.