bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of vibration vs non-vibration foam rolling techniques on flexibility, dynamic balance and perceived joint stability after fatigue
Authors: de Benito Ana María , Valldecabres Raúl , Ceca Diego , Richards Jim , Barrachina Igual Joaquín , Pablos Ana 
Published: November 2019

Background
Foam roller and vibration techniques are currently used to assist in recovery after fatigue. The main purpose of this study was to determine the effects of the use of a foam roller with and without vibration on dynamic balance, ankle dorsiflexion, hamstring and lumbar spine flexibility and perceived knee and ankle stability after an induced fatigue protocol.

Methods
A total of 24 healthy recreationally active participants (17 males and seven females) were recruited to a randomized cross over trial consisting of; no treatment (NT), foam roller treatment (FR) and vibration foam roller treatment (VFR). The assessments included; the Sit & Reach test, Y balance test and post-treatment perceived knee and ankle stability. Measurements were taken after a standardized warm up (baseline) and repeated following an exercise-induced muscle fatigue protocol consisting of repeated lunges until volitional fatigue. The three treatment conditions were assessed on three separate days in a randomized order. A 3 × 3 repeated measures ANOVA was used to investigate differences between the three treatments over the three time points and a one factor repeated measures ANOVA was used to determine any differences between treatments using the Global Rate of Change scale when considering perceived stability.

Results
FR and VFR conditions both showed a greater ankle dorsiflexion range of motion (ROM) (p < 0.001), greater posteromedial and posterolateral reach distances (p < 0.001) and a better knee and ankle perceived stability (p < 0.001) when compared to the NT condition. A trend toward significance was observed in the hamstring and lumbar spine flexibility (p = 0.074) in both treatment conditions when compared to the NT condition. However, no differences were seen between the FR and VFR conditions.

Conclusions
Both FR conditions seem to assist in exercise-induced muscle fatigue recovery with improvements in ROM, balance and perceived stability.
Identification of novel genes associated with litter size of indigenous sheep population in Xinjiang, China using specific-locus amplified fragment sequencing technology
Authors: Ma Haiyu , Fang Chao , Liu Lingling , Wang Qiong , Aniwashi Jueken , Sulaiman Yiming , Abudilaheman Kezierkailedi , Liu Wujun 
Published: November 2019

Background
There are abundant sheep breed resources in the Xinjiang region of China attributing to its diverse ecological system, which include several high-litter size sheep populations. Previous studies have confirmed that the major high prolificacy gene cannot be used to detect high litter size. Our research team found a resource group in Pishan County, southern Xinjiang. It showed high fertility with an average litter size of two to four in one birth, excellent breast development, and a high survival rate of lambs. In the present study, we used this resource as an ideal sample for studying the genetic mechanisms of high prolificacy in sheep.

Methods
Indigenous sheep populations from Xinjiang, with different litter sizes, were selected for the research, and specific-locus amplified fragment sequencing (SLAF-seq) technology was used to comprehensively screen single nucleotide polymorphisms (SNPs) from the whole genome that may cause differences in litter size. Novel genes associated with litter size of sheep were detected using genome-wide association studies (GWAS), providing new clues revealing the regulation mechanism of sheep fecundity. Candidate genes related to ovulation and litter size were selected for verification using Kompetitive Allele Specific polymerase chain reaction (KASP) cluster analysis.

Results
We identified 685,300 SNPs using the SLAF-seq technique for subsequent genome-wide analysis. Subsequently, 155 SNPs were detected at the genome-wide level. Fourteen genes related to sheep reproduction were notated: COIL, SLK, FSHR, Plxna3, Ddx24, CXCL12, Pla2g7, ATP5F1A, KERA, GUCY1A1, LOC101107541, LOC101107119, LOC101107809, and BRAF. Based on literature reports, 30 loci of seven genes and candidate genes (CXCL12, FSHR, SLK, GUCY1A1, COIL, LOC101107541, and LOC101107119) related to ovulation and litter size were selected for verification using KASP cluster analysis. Among them, nine loci of three genes were successfully genotyped. Three loci of FSHR (GenBank ID: 443299, g. 75320741G>A site), GUCY1A1 (GenBank ID: 101110000, g. 43266624C>T site), and COIL (GenBank ID: 101123134, g. 7321466C>G site) were found to be significantly or extremely significantly associated with litter size. These three loci are expected to be used as molecular markers to determine differences in litter size in sheep.
Molecular Therapeutic Cancer Peptides: A Closer Look at Bovine Lactoferricin
Authors: Sadiq Idris Zubairu , Babagana Kamaluddeen , Danlami Dauda , Abdullahi Lawi Isa , Khan Amir Riyaz 
Published: November 2019

Background: In spite of the progress that was recorded in the development of anticancer drugs, challenges continue to rises particularly due to resistance of the cancer chemotherapies and low sensitivity of the commercially available anticancer drugs.
Methodology: collection and review of available literatures.
Results: Bovine lactoferricin (LfcinB) is a cationic peptide with broad spectrum antimicrobial and anticancer activity. It also shows promising activity against many varieties of cancer cells including colon carcinoma cells, melanoma, fibrosarcoma, monocytic leukemic cells and neuroblastoma. It is unaffected by multidrug resistance mechanisms seen with conventional chemotherapeutic drugs, and displays a higher specificity for cancer cells versus normal cells in comparison to conventional chemotherapy.
Conclusion: LfcinB exhibit strong antitumor activity effectively penetrating cell membrane, activating caspases and induce apoptosis to cancer cells.
Comparative Study of the Effect of Aqueous Extract of Entandrophragma angolense and Vitamin B12 on Induced Hemolytic Anemia and Inflammation in the Wistar Rat
Authors: Mansour Adeoti, Franck , Aurélie Yenon, Achiè , Jonas Vanie, Bi Foua , Bidié Alain, Dit Philippe , Joseph Djaman, Allico 
Published: November 2019

Entandrophragma angolense is a plant of the family Meliaceae of African pharmacopoeia. It is used in traditional medicine as an analgesic and anti-inflammatory in cases of abdominal or hips pain. The aim of this study is to compare the effect of oral administration of aqueous extract of E. angolense and vitamin B12 on hemolytic induced anemia and inflammation in the rat. Anemia was induced by intraperitoneal administration of phenylhydrazine at the dose of 40 mg / kg / day for two days in 24 Wistar rats, followed by aqueous extract of E. angolense and vitamin B12 chosen as reference molecule administered orally to rats once a day for 28 days. A biological assessment on blood count, CRP and the study of secondary metabolites of the aqueous extract of E. angolense have been realized. The results obtained showed the existence of anemia characterized by a significant decrease (p <0.01) of the hemoglobin level of 42.80% ± 3.09 and red blood cells of 44.21% ± 3.16 in rats, and inflammation revealed by the significantly elevated CRP assay (p <0.05). The aqueous extract of E. angolense at the dose of 200 mg / kg increased significantly (p <0.001) the number of red blood cells and hemoglobin at the 4th week of treatment compared to the vitamin B12.
In conclusion, the aqueous extract of the plant has an anti-inflammatory and anti-anemic effect. This double physiological action could come from the phytochemicals (flavonoids, saponins and alkaloids) contained in the extract.
Investigation of the Association of FOLH1 rs61886494 and DISC1 rs12133766 loci in Iranian Schizophrenia Patients
Authors: Parisa Azizi , Faryal Azizi , Fahimeh Nemati 
Published: November 2019

Background: The aim of this study was to identification of polymorphisms of FOLH1 and DISC1 genes in Iranian patients with schizophrenia.
Materials and Methods: In this case-control study, 50 patients with schizophrenia and 50 healthy controls were evaluated. PCR-RFLP and Tetra-ARMS method used for detection of FOLH1 and DISC1 gene respectively in both of patients and control groups.
Results: The frequency of CC, CT, and TT genotypes for FOLH1 gene in rs61886494 locus in schizophrenic patients was 92%, 8%, and 0%, respectively, and in healthy subjects, 94%, 0%, and 6%, respectively. The frequency of DISC gene in GG genotype was higher than that of normal people and frequency of GA genotype was lower than normal subjects. In addition, the genotype AA was identified only in patients.
Conclusion: For FOLH1 gene in rs61886494 locus, the frequency of CC and TT genotypes in patients was 2% and 6% lower in healthy people, while CT genotype in patients was 8% higher in healthy people. Interestingly, TT genotype was not observed in patients and CT genotype in healthy people was not observed. Regarding the DISC1 gene, the results showed that the frequency of homozygous GG and GA homozygote genotypes in the patients was higher in the rs12133766 locus, while the heterozygote GA was high in healthy subjects and was not observed in patients. Therefore, the result of this study in our country can provide suitable method for diagnosis and prevention of schizophrenia patients.
The Efficiency and Effectiveness of Open Pollination in Musa Breeding
Authors: Wilson Victoria , Tenkouano Abdou 
Published: November 2019

Aims: This field experiment was conducted to determine if hand and open pollination methods affected performances of Musa progenies from 4x - 2x crosses and to identify promising progenies for recurrent selection.
Study Design: The experimental design was a randomized complete block design with two replications of 6 plants per genotype.
Place and Duration of Study: International Institute of Tropical Agriculture (IITA) High Rainfall Station, Onne (4º51’N, 7º03’E, 10 m above sea level), in Rivers State, South-south Nigeria for 24 months.
Methodology: Two-month old seedlings of hand pollinated (6 diploid, 6 tetraploid) and open pollinated (6 diploid, 6 tetraploid) progenies, along with parental clones (2x) and (4x) of each genotype were planted at 3 m x 2 m spacing. Data on phenology, vegetative growth, yield and yield characters were collected at flowering and harvest over three crop cycles. Genotypes were partitioned into 5 clusters assayed by means of orthogonal contrasts to compare the performance of progenies from both pollination methods.
Results: Pollination methods produced no significant (P = .05) differences, unfavourable effects or reduction in performance of economically important yield and yield components of 4x and 2x progenies of similar genotype. Some significant (P = .05) linear correlations and relationships between phenological and vegetative traits; and yield and yield components changed with pollination methods and ploidy levels but did not affect final outcomes. Promising open pollinated diploids include the early maturing TMP2x 2829-62OP; and for high yield and yield components measured, TMB2x 8084-2OP and TMP2x 1448-1OP. Promising open pollinated tetraploids include TMP4x 7002-1OP and TMP4x 2796-5OP.
Conclusion: Open pollination did not result in unfavorable effects or reduction in performance of economically important yield and yield components in progenies of similar genotypes. Therefore, open pollination could be considered for Musa breeding. This will reduce cost, labour, time and stress involved in Musa improvement.
Comparative Analysis for Internal Fixations of Pauwels II by Biomechanical Finite Element Method
Authors: Peng Matthew Jian-Qiao , Ju Xiangyang , Chen Hai-Yan , Bo Bai , Li XinXu 
Published: November 2019

Purpose:
A series models of surgical internal fixation for femoral neck fracture of Pauwels II will be constructed by an innovative approach of finite element so as to determine the most stable fixation by comparison of their biomechanical performance.

Method:
Seventeen specimens of proximal femurs scanned by computed tomography in Digital Imaging and Communications in Medicine (DICOM) format were input onto Mimics rebuilding 3D models; their stereolithography (STL) format dataset were imported into Geomagic Studio (3D Systems, Rock Hill, South Carolina) for simulative osteotomy and non-uniform rational basis spline kartograph; the generated IGS dataset were interacted by UG to fit simulative 3D-solid models; 3 sorts of internal fixators were expressed in 3D model by ProE (PTC, Boston, Connecticut) program virtually. Processed by HyperMesh (Altair, Troy, Michigan), all compartments (fracture model + internal immobilization) were assembled onto 3 systems actually as: Dynamic hip screw (DHS) / Lag screw (LS) / DHS+LS. Eventually, a numerical model of finite elemental analysis was exported to ANSYS for solution.

Result:
Three models of internal fixations for femoral neck fracture of Pauwels II were established and validated effectively, the stress and displacement of each internal pin were analyzed, the advantages of each surgical therapy for femoral neck fracture of Pauwels II were compared and demonstrated synthetically as: “The contact stress of 3-LS-system was checked to be the least; the interfragmentary displacement of DHS+1-LS assemblages was assessed to be the least.”

Conclusion:
3-LS-system is recommended to be a clinical optimization for Pauwels II femoral neck facture, by this therapeutic fixation mechanically, breakage of fixators, or secondary fracture rarely occurs.
A data mining approach for classification of orthostatic and essential tremor based on MRI‐derived brain volume and cortical thickness
Authors: Benito‐León Julián , Louis Elan D. , Mato‐Abad Virginia , Sánchez‐Ferro Alvaro , Romero Juan P. , Matarazzo Michele , Serrano J. Ignacio 
Published: November 2019

AbstractObjectiveOrthostatic tremor (OT) is an extremely rare, misdiagnosed, and underdiagnosed disorder affecting adults in midlife. There is debate as to whether it is a different condition or a variant of essential tremor (ET), or even, if both conditions coexist. Our objective was to use data mining classification methods, using magnetic resonance imaging (MRI)‐derived brain volume and cortical thickness data, to identify morphometric measures that help to discriminate OT patients from those with ET.MethodsMRI‐derived brain volume and cortical thickness were obtained from 14 OT patients and 15 age‐, sex‐, and education‐matched ET patients. Feature selection and machine learning methods were subsequently applied.ResultsFour MRI features alone distinguished the two, OT from ET, with 100% diagnostic accuracy. More specifically, left thalamus proper volume (normalized by the total intracranial volume), right superior parietal volume, right superior parietal thickness, and right inferior parietal roughness (i.e., the standard deviation of cortical thickness) were shown to play a key role in OT and ET characterization. Finally, the left caudal anterior cingulate thickness and the left caudal middle frontal roughness allowed us to separate with 100% diagnostic accuracy subgroups of OT patients (primary and those with mild parkinsonian signs).ConclusionsA data mining approach applied to MRI‐derived brain volume and cortical thickness data may differentiate between these two types of tremor with an accuracy of 100%. Our results suggest that OT and ET are distinct conditions.
Single‐Cell Analysis Using Droplet Microfluidics
Authors: Matuła Kinga , Rivello Francesca , Huck Wilhelm T. S. 
Published: November 2019

AbstractDroplet microfluidics has revolutionized the study of single cells. The ability to compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide range of strategies to extract information at the genomic, transcriptomic, proteomic, or metabolomic level from large numbers of individual cells. Studying the different molecular landscapes at single‐cell resolution has provided the authors with a detailed picture of intracellular heterogeneity and the resulting changes in cellular phenotypes. In addition, these technologies have aided in the discovery of rare cells in tumors or in the immune system, and left the authors with a deeper understanding of the fundamental biological processes that determine cell fate. This review aims to provide a detailed overview of the various droplet microfluidic strategies reported in the literature, taking into account the sometimes subtle differences in workflow or reagents that enable or improve certain protocols. Specifically, approaches to targeted‐ and whole‐genome analysis, as well as whole‐transcriptome profiling techniques, are reviewed. In addition, an up‐to‐date overview of new methods to characterize and quantify single‐cell protein levels, and of developments to screen secreted molecules such as antibodies, cytokines, or metabolites at the single‐cell level, is provided.
Out‐of‐Plane Polarization in Bent Graphene‐Like Zinc Oxide and Nanogenerator Applications
Authors: Tan Dan , Willatzen Morten , Wang Zhong Lin 
Published: November 2019

AbstractHighly efficient piezoelectric nanogenerator operation is demonstrated based on dynamic bending of graphene‐like ZnO nanosheets. Energy is harvested by an external resistor by virtue of a strong time‐varying piezoelectric polarization component perpendicular to the graphene‐like ZnO plane. It is shown analytically and verified numerically using molecular dynamics simulations that the m2 point group of flat graphene‐like ZnO is reduced to monoclinic m symmetry for bent graphene‐like ZnO. The latter symmetry allows for a nonzero and large piezoelectric polarization component perpendicular to the plane of the 2D structure. The numerical results confirm that flexoelectric effects are negligible subject to graphene‐like ZnO bending operation.
One‐Step Rapid Fabrication of Cell‐Only Living Fibers
Authors: Sousa Ana Rita , Martins‐Cruz Cláudia , Oliveira Mariana B. , Mano João F. 
Published: November 2019

AbstractCellular aggregates are used as relevant regenerative building blocks, tissue models, and cell delivery platforms. Biomaterial‐free structures are often assembled either as 2D cell sheets or spherical microaggregates, both incompatible with free‐form deposition, and dependent on challenging processes for macroscale 3D upscaling. The continuous and elongated nature of fiber‐shaped materials enables their deposition in unrestricted multiple directions. Cellular fiber fabrication has often required exogenously provided support proteins and/or the use of biomaterial‐based sacrificial templates. Here, the rapid (<24 h) assembly of fiberoids is reported: living centimeter‐long scaffold‐free fibers of cells produced in the absence of exogenous materials or supplements. Adipose‐derived mesenchymal stem cell fiberoids can be easily modulated into complex multidimensional geometries and show tissue‐invasive properties while keeping the secretion of trophic factors. Proangiogenic properties studied on a chick chorioallantoic membrane in an ovo model are observed for heterotypic fiberoids containing endothelial cells. These micro‐to‐macrotissues may find application as morphogenic therapeutic and tissue‐mimetic building blocks, with the ability to integrate 3D and 4D full biological materials.
Through Space Charge Transfer for Efficient Sky‐Blue Thermally Activated Delayed Fluorescence (TADF) Emitter with Unconjugated Connection
Authors: Wang Ya‐Kun , Huang Chen‐Chao , Ye Hao , Zhong Cheng , Khan Aziz , Yang Sheng‐Yi , Fung Man‐Keung , Jiang Zuo‐Quan , Adachi Chihaya , Liao Liang‐Sheng 
Published: November 2019

AbstractThrough‐space charge transfer, which exists in nonconjugation linker based thermally activated delayed fluorescence (TADF) materials, excites chemists to explore more possibilities in organic light‐emitting diodes (OLEDs). Herein, an sp3‐hybrid carbon‐centered donor–σ–acceptor type chromophore, QAFCN, is tentatively developed by exploring bi‐acridine based electron‐donor, i.e., 5,5‐dimethyl‐5,9‐dihydroquinolino[3,2,1‐de]acridine (QA). It is interesting to find that the QA moiety shows downshift in highest occupied molecular orbital because of its deformed geometry, which makes it qualified for sky‐blue electroluminescence emission. Together with the blue‐shift, enhanced photoluminescence quantum yield and faster reverse intersystem crossing rate are also observed in QAFCN, which can be attributed to the close through‐space distance between donor/acceptor. As compared to the ACRFLCN without these special features, QAFCN has obvious TADF property and achieves efficient OLEDs of ≈18% external quantum efficiency.
Recent Progress in Two‐Dimensional Ferroelectric Materials
Authors: Guan Zhao , Hu He , Shen Xinwei , Xiang Pinghua , Zhong Ni , Chu Junhao , Duan Chungang 
Published: November 2019

AbstractThe investigation of two‐dimensional (2D) ferroelectrics has attracted significant interest in recent years for applications in functional electronics. Without the limitation of a finite size effect, 2D materials with stable layered structures and reduced surface energy may go beyond the presence of an enhanced depolarization field in ultrathin ferroelectrics, thereby opening a pathway to explore low‐dimensional ferroelectricity, making ultra‐high‐density devices possible and maintaining Moore's Law. Although many theoretical works on potential 2D ferroelectric materials have been conducted, much still needs to be accomplished experimentally, as it is rare for 2D ferroelectric materials to be proven and plenty of 2D ferroelectrics are waiting to be discovered. First, experimental and theoretical progress on 2D ferroelectric materials, including in‐plane and out‐of‐plane, is reviewed, followed by a general introduction to various characterization methods. Intrinsic mechanisms associated with promising 2D ferroelectric materials, together with related applications, are also discussed. Finally, an outlook for future trends and development in 2D ferroelectricity are explored. Researchers can use this to obtain a basic understanding of 2D ferroelectric materials and to build a database of progress of 2D ferroelectrics.
Thin, Skin‐Integrated, Stretchable Triboelectric Nanogenerators for Tactile Sensing
Authors: Liu Yiming , Wang Lingyun , Zhao Ling , Yao Kuanming , Xie Zhaoqian , Zi Yunlong , Yu Xinge 
Published: November 2019

AbstractRecent advances in thin, soft skin‐integrated electronics have brought many opportunities in the wearable technics. A simple platform with the functionality of self‐powering for epidermal electronics is reported. These electronics can generate electricity from external mechanical stresses that associates with triboelectric effect, and therefore afford excellent performance in tactile sensing and energy harvesting. Combined advances in materials and mechanics of the skin‐integrated electronics with high efficiency energy harvesting techniques, triboelectric nanogenerators (TENGs) in an epidermal format is realized for the first time. The dots‐distributed electrode pattern allows these electronics exhibiting excellent flexibility and stretchability, distinguishing a broad range of pressures that are relevant to normal body motions. The electricity output of the epidermal device from simple finger tapping modes can achieve >60 V of voltage and >1 µA of current, which is sufficient to light up 15 small light‐emitting diodes. Furthermore, the authors also report a 4 × 4 sensor array based on these TENGs, and demonstrate a skin‐like electronics for real‐time motion monitoring and tactile mapping.