bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomes for facial repair, regeneration and rejuvenation: comparison between different commercial products
Authors: Salvaggio Antonio , Privitera Anna , Ferruggia Greta , Zimbone Massimo , Brundo Maria Violetta 
Published: December 2024

AbstractExosomes have been studied as a potential therapeutic option for improving skin texture. The mode of action of exosomes for skin texture likely involves several mechanisms, including stimulation of collagen production, promotion of skin cell proliferation; reduction of oxidative stress; regulation of inflammatory and antioxidant responses; extracellular matrix remodeling, which is important for maintaining skin structure and function. Aim of this study is to compare thein vitroeffects of some commercial products that use new technologies containing plant derived nanovesicles fromRosa damascenacallus,Centella asiaticacallus,Euphorbia supinastem and exosomes from bovine colostrum passively loaded with growth factors and cytokines purified from bovine colostrum, for facial repair, regeneration and rejuvenation. Of all the products tested, only product containing exosomes purified from colostrum gave very encouraging results in terms of effects on proliferation, cell viability and wound repair. The other products tested gave results comparable to the untreated samples.
Adoption challenges of sensing technologies in construction project management in sub-Saharan African countries
Authors: Ibrahim Kabir , Amoah Christopher , Simpeh Fredrick , William Justice 
Published: December 2024

PurposeThe use of technology has increased in the industrial sector over the years. This has propelled the construction industry to follow suit by enhancing construction technology to experience the associated benefits. However, technology adoption in the construction environment has not been smoothly implemented. This article aimed to determine the obstacles to adopting sensing technology in managing construction projects in three major sub-Saharan African nations.Design/methodology/approachThe study adopted a quantitative approach using structured questionnaires. The questionnaire link was sent to construction professionals in Nigeria, Ghana and South Africa, of which 120 were responded. Data received were analysed using the Statistical Package for Special Science (SPSS) to generate mean scores and the Kruskal–Wallis test and component analysis.FindingsThe results show that construction professionals face herculean tasks when adopting sensing technology in construction project management. Some prominent challenges are initial cost, lack of proper information technology (IT) infrastructure, power supply, lack of well-trained staff, maintenance cost, operating cost, cost of training, technology immaturity and resistance to change. The results also revealed that adoption challenges differ in these three countries, which may be attributable to the economic development level.Practical implicationsDespite the importance of sensing devices in construction industries, the study has revealed that Sub-Saharan African countries face enormous challenges in adopting them in project management and execution. This situation may hinder the industry’s ability to benefit from this technology in terms of efficiency, effectiveness and safety management, which may ultimately affect their cash flows.Originality/valueThe study provides a valuable understanding of the challenges encountered in implementing sensing technology in the construction sector in major countries within sub-Saharan Africa. These findings will enable the countries examined and other African countries to become cognizant of the challenges and implement strategies to overcome them, thereby enhancing performance in the construction industry.
Ventricular Tachycardia Possibly Originated From the His‐Purkinje System May Result From Aconitine Poisoning
Authors: Ni Honglin , Zhai Changlin , Pan Haihua 
Published: December 2024

ABSTRACTAccurate diagnosis of wide QRS complex tachycardia (WCT) with the earliest ventricular origin potentially located in the His‐Purkinje system, and exploration of its etiology are helpful for further diagnosis and treatment. This article finds through specific cases that one of the possible etiologies is aconitine poisoning.
Comparable outcomes among etiologies in early‐stage hepatocellular carcinoma: Analysis of a nationwide registry in Japan
Authors: Okushin Kazuya , Tateishi Ryosuke , Hirakawa Shinya , Tachimori Hisateru , Uchino Koji , Nakagomi Ryo , Yamada Tomoharu , Nakatsuka Takuma , Minami Tatsuya , Sato Masaya , Fujishiro Mitsuhiro , Hasegawa Kiyoshi , Eguchi Yuichiro , Kanto Tatsuya , Yoshiji Hitoshi , Izumi Namiki , Kudo Masatoshi , Koike Kazuhiko 
Published: December 2024

AbstractAimTumor stage and liver function determine the prognosis of patients with hepatocellular carcinoma (HCC). However, prognosis may differ depending on etiology. This study aimed to investigate etiological differences in the prognosis of patients with HCC.MethodsWe enrolled patients initially diagnosed with HCC and curatively treated with resection or ablation between 2018 and 2021 from a Japanese nationwide registry. The patients were grouped into hepatitis B (HBV), hepatitis C (HCV), and non‐B, non‐C. Viral status was also assessed. The end‐points were overall survival and decompensation‐free survival. We applied inverse probability of treatment weighting to draw adjusted Kaplan–Meier curves.ResultsOf the 4568 patients, 3546 underwent resection and 1022 underwent ablation. Hepatitis B virus DNA was undetectable in 45.2% of patients with HBV, and a sustained virologic response was achieved in 42.7% of patients with HCV at the initial diagnosis of HCC. In the resection cohort (13.9% HBV, 29.4% HCV, and 56.0% non‐B, non‐C), the adjusted overall survival showed no significant difference between non‐B, non‐C versus HBV (p = 0.57, log‐rank test) and non‐B, non‐C versus HCV (p = 0.68). Decompensation‐free survival was also similar among etiologies. In the ablation cohort (11.1% HBV, 44.2% HCV, and 43.9% non‐B, non‐C), there was also no significant difference in adjusted survival among etiologies.ConclusionPrognosis of patients with HCC was similar among different etiologies after adjusting for tumor stage and liver function. Nearly half of the patients with viral hepatitis achieved viral suppression or eradication at the initial diagnosis of HCC in Japan.
Autologous Cell‐Free Fat Extract: A Novel Approach for Infraorbital Rejuvenation—A Pilot Study
Authors: Cai Yizuo , Jia Zhuoxuan , Gu Jiancheng , Kang Bijun , Li Wei , Zhang Wenjie 
Published: December 2024

ABSTRACTBackground and ObjectiveCEFFE (Cell‐free fat extract) treatment for periocular fine lines requires thorough clinical evaluation to determine its efficacy and safety in enhancing skin quality. The research enrolled 10 healthy female participants aged 31–58, focusing on skin texture, elasticity, and barrier function.Methods and ResultsCEFFE treatment demonstrated significant benefits, with notable improvements observed as early as 3 months posttreatment, which continued throughout the 12‐month follow‐up period. Objective assessments revealed reductions in SEr% and SEw%, indicative of reduced skin roughness and wrinkles, particularly pronounced after the third month of treatment. Enhanced skin elasticity, as indicated by improvements in R2%, R5%, and R7%, was observed, with the most significant enhancements noted at the 6‐month follow‐up. Furthermore, TEWL decreased consistently, highlighting CEFFE's potential in maintaining the skin's barrier function and moisture retention. High patient satisfaction levels, with 70% expressing satisfaction ranging from satisfied to very satisfied, underscored CEFFE's clinical significance.ConclusionsCEFFE demonstrates potential as an effective and safe intervention for addressing periocular fine lines, providing a solution for fine lines while ensuring skin health (ChiCTR1900024329).
Evaluating the Efficacy of Combined Platelet‐Rich Plasma and Microneedling for Aesthetic Rejuvenation of the Periorbital Area: A Randomized, Blinded Cohort Study
Authors: Hassan Sally Al , Saade Dana S. , Kurban Mazen , Rahal Jihane Abou , Alameddine Ramzi M. 
Published: December 2024

ABSTRACTIntroductionMicroneedling, a technique involving controlled dermal microwounding, and platelet‐rich plasma (PRP) injections are both employed for skin rejuvenation. While both treatments individually show promise, limited research has explored their combined efficacy. This study aimed to evaluate the effectiveness of combining PRP injections with microneedling for aesthetic concerns around the eyes under standardized conditions.MethodsThis single‐center, longitudinal cohort study was conducted from October 2017 to October 2020. Thirteen adult participants (mean age 35, 92.3% female) with aesthetic concerns in the periorbital area underwent combined PRP and microneedling treatment. Standardized photographs were taken preprocedure, at 1 week, and 3 months. Photographs were evaluated by three blinded dermatologists using validated photonumeric scales. Participants completed a self‐assessment questionnaire at the 1‐week follow‐up regarding skin homogeneity, texture, pigmentation, and wrinkles.ResultsSelf‐reported outcomes indicated perceived improvements in skin homogeneity (72.7% reporting mild or significant improvement) and texture (81.8% reporting mild, moderate, or significant improvement). Improvements in pigmentation and wrinkles were less pronounced. Dermatologists' assessments revealed no significant differences in skin attributes before and after treatment.ConclusionDespite subjective reports of improvement, objective evaluations by independent dermatologists did not demonstrate significant changes. Variability in outcomes might be attributed to differences in study design, treatment protocols, and assessment methods. Further research with larger sample sizes and multiple treatment sessions is needed to better understand the benefits of combining PRP with microneedling for periorbital rejuvenation.
An R2R3‐type MYB transcription factor, GmMYB77, negatively regulates isoflavone accumulation in soybean [Glycine max (L.) Merr.]
Authors: Liu Yitian , Zhang Shengrui , Li Jing , Muhammad Azam , Feng Yue , Qi Jie , Sha Dan , Hao Yushui , Li Bin , Sun Junming 
Published: December 2024

SummarySoybean [Glycine max (L.) Merr.] is an exceptionally rich in isoflavones, and these compounds attach to oestrogen receptors in the human body, lessening the risk of breast cancer and effectively alleviating menopausal syndrome symptoms. Uncovering the molecular mechanisms that regulate soybean isoflavone accumulation is crucial for enhancing the production of these compounds. In this study, we combined bulk segregant analysis sequencing (BSA‐seq) and a genome‐wide association study (GWAS) to discover a novel R2R3‐MYB family gene, GmMYB77, that regulates isoflavone accumulation in soybean. Using the soybean hairy root transient expression system, we verified that GmMYB77 inhibits isoflavone accumulation. Furthermore, knocking out GmMYB77 significantly increased total isoflavone (TIF) content, particularly malonylglycitin, while its overexpression resulted in a notable decrease in contents of malonylglycitin and TIF. We found that GmMYB77 can directly binds the core sequence GGT and suppresses the expression of the key isoflavone biosynthesis genes Isoflavone synthase 1 (GmIFS1), Isoflavone synthase 2 (GmIFS2), Chalcone synthase 7 (GmCHS7) and Chalcone synthase 8 (GmCHS8) by using dual‐luciferase assays, electrophoretic mobility shift assays and yeast one‐hybrid experiments. Natural variations in the promoter region of GmMYB77 affect its expression, thereby regulating the malonylglycitin and TIF contents. Hap‐P2, an elite haplotype, plays a pivotal role in soybean breeding for substantially enhanced isoflavone content. These findings enhance our understanding of the genes influencing soybean isoflavone content and provide a valuable genetic resource for molecular breeding efforts in the future.
Construction and infection characteristics of a GFP‐labelled Botryosphaeria dothidea causing kiwifruit soft rot disease
Authors: Song Jinhuang , Shu Yiyang , Zheng Yanfei , Zhao Zhibo , Long Youhua , Fan Rong 
Published: December 2024

AbstractSoft rot disease caused by Botryosphaeria dothidea is one of the most important fungal diseases affecting kiwifruit production. The symptoms of the disease generally appear during the fruit storage period, resulting in difficult disease management and serious economic losses. Therefore, the characterization of the infection mechanism needs to be explored to deepen our understanding of the pathogen and the disease. In this study, a highly pathogenic strain JY‐1, causing kiwifruit soft rot, was isolated and identified as B. dothidea. A polyethylene glycol‐mediated genetic transformation system for B. dothidea JY‐1 was established, enabling the successful acquisition of a green fluorescent protein (GFP)‐labelled strain with stable inheritance. The GFP‐labelled transformant Bd13 was generated and used in the research, and the infection process of kiwifruit tissues was studied through wound inoculation essays and fluorescence labelling microscopy. The results of the present study indicate that the transformant Bd13 not only damaged the kiwifruit fruits but also effectively infected and colonized the leaves and shoots of kiwifruit plants, causing shoot blight and leaf spot. These findings contribute to a deeper understanding of the pathogenic mechanisms underlying kiwifruit soft rot and lay a foundation for further investigations into the interaction between the soft rot pathogen B. dothidea and kiwifruit.
CRISPR/Cas9‐driven double modification of grapevine MLO6‐7 imparts powdery mildew resistance, while editing of NPR3 augments powdery and downy mildew tolerance
Authors: Moffa Loredana , Mannino Giuseppe , Bevilacqua Ivan , Gambino Giorgio , Perrone Irene , Pagliarani Chiara , Bertea Cinzia Margherita , Spada Alberto , Narduzzo Anna , Zizzamia Elisa , Velasco Riccardo , Chitarra Walter , Nerva Luca 
Published: December 2024

SUMMARYThe implementation of genome editing strategies in grapevine is the easiest way to improve sustainability and resilience while preserving the original genotype. Among others, the Mildew Locus‐O (MLO) genes have already been reported as good candidates to develop powdery mildew‐immune plants. A never‐explored grapevine target is NPR3, a negative regulator of the systemic acquired resistance. We report the exploitation of a cisgenic approach with the Cre‐lox recombinase technology to generate grapevine‐edited plants with the potential to be transgene‐free while preserving their original genetic background. The characterization of three edited lines for each target demonstrated immunity development against Erysiphe necator in MLO6‐7‐edited plants. Concomitantly, a significant improvement of resilience, associated with increased leaf thickness and specific biochemical responses, was observed in defective NPR3 lines against E. necator and Plasmopara viticola. Transcriptomic analysis revealed that both MLO6‐7 and NPR3 defective lines modulated their gene expression profiles, pointing to distinct though partially overlapping responses. Furthermore, targeted metabolite analysis highlighted an overaccumulation of stilbenes coupled with an improved oxidative scavenging potential in both editing targets, likely protecting the MLO6‐7 mutants from detrimental pleiotropic effects. Finally, the Cre‐loxP approach allowed the recovery of one MLO6‐7 edited plant with the complete removal of transgene. Taken together, our achievements provide a comprehensive understanding of the molecular and biochemical adjustments occurring in double MLO‐defective grape plants. In parallel, the potential of NPR3 mutants for multiple purposes has been demonstrated, raising new questions on its wide role in orchestrating biotic stress responses.
Ditopic Centrosymmetric Mercaptobenzothiazole Dilithium Salts: From the Molecular Complex to Luminescent 1D Coordination Polymers
Authors: Rogozhin A. F. , Ilichev V. A. , Silantyeva L. I. , Kozlova E. A. , Fukin G. K. , Bochkarev M. N. 
Published: December 2024

Abstract
The reaction of lithium amide LiN(Si(Me)3)2 and ditopic heterocyclic ligand benzo[1,2-d:4,5-d′]bis(thiazole)-2,6(3H,7H)-dithione (H2L) in dimethoxyethane (DME) affords the binuclear molecular complex Li2L(DME)4 (I). New compounds [Li2L(DMSO)4·(DMSO)2]n (II) and [Li2L(DMSO)4·(THF)2]n (III) are prepared by the recrystallization of compound I using a DMSO–diethyl ether or DMSO/THF mixture of solvents, respectively. According to the XRD data, these compounds are one-dimensional Coordination polymer (CPs) differed by the arrangement of the bis(thiazole) fragments relative to each other and the Li2O2 fragment in the polymer chain, which affects the luminescence properties. The molecular structures of compounds I–III are determined by XRD (CIF files CCDC nos. 2334192 (I), 2334193 (II), and 2334194 (III)).
CSF GPNMB in Parkinson's disease: A potential association with age and microglial activation
Authors: Zhu Xi-Chen , Mizutani Yasuaki , Ohdake Reiko , Tatebe Harutsugu , Maeda Toshiki , Shima Sayuri , Ueda Akihiro , Ito Mizuki , Ito Shinji , Tokuda Takahiko , Watanabe Hirohisa 
Published: December 2024

Background Recent evidence suggests a link between glycoprotein non-metastatic melanoma protein B (GPNMB) and Parkinson's disease (PD) pathogenesis. Although elevated plasma GPNMB levels associated with disease severity have been reported in PD, cerebrospinal fluid (CSF) alterations remain elusive. Objective To explore CSF GPNMB alterations and its clinical significance in PD. Methods This study enrolled 118 sporadic PD patients and 40 controls. We examined the potential associations between CSF GPNMB levels and the clinical characteristics or biomarkers of neurodegenerative pathogenesis. Results PD patients had higher CSF GPNMB levels than controls ( p = 0.0159). In the PD group, CSF GPNMB levels correlated with age (age at examination: rs = 0.2511, p = 0.0061; age at onset: rs = 0.2800, p = 0.0021) and the severity of motor and cognitive dysfunction (MDS-UPDRS III score: rs = 0.1998, p = 0.0347; Mini-Mental State Examination score: rs = −0.1922, p = 0.0370). After correcting for multiple comparisons, the correlation with age at onset remained significant. CSF GPNMB levels were also positively correlated with CSF soluble triggering receptor expressed on myeloid cells 2 (sTREM2) levels in both the PD ( rs = 0.3582, p < 0.0001) and control ( rs = 0.4743, p = 0.0023) groups. Furthermore, multiple regression analysis revealed CSF sTREM2 level as the strongest determinant of CSF GPNMB levels in the PD group (t-value = 3.49, p = 0.0007). Conclusions Elevated CSF GPNMB levels, linked with age and microglial activation, may be a valuable marker for understanding the interplay between aging, neuroinflammation, and PD pathology.
Clinical prognostic factors in progressive supranuclear palsy: Implications for clinical trials
Authors: Marchand Félix , Blaise Anne-Sophie , Defebvre Luc , Cailliau Emeline , Bombois Stéphanie , Devos David , Moreau Caroline 
Published: December 2024

Background Progressive supranuclear palsy (PSP) is a rare neurodegenerative disease with diverse clinical phenotypes, prompting the development of new diagnostic criteria known as the MDS-PSP classification. However, little is known about the prognostic value of this classification in order to better stratify patients for the clinical trials. Objective To assess the impact of the different clinical phenotypes according to the MDS-PSP classification on prognosis using the clinical milestones of death, severe dysphagia, institutionalization, and need for walking aid. Methods A prospective cohort of 205 PSP patients from Lille University Hospital was analyzed retrospectively. Patients were classified into different MSD-PSP phenotypes according to their clinical presentation after 3 years of follow-up. The milestones of death, severe dysphagia, institutionalization, and need for walking aid were recorded, and a survival analysis was performed to describe the prognosis of each disease presentation. Results Median survival time was 6.4 (interquartile range (IQR): 4.8–8.6) years and mean diagnostic delay from symptom onset was 38.1 ± 22.5 months. PSP Richardson Syndrome (PSP-RS) had a poorer survival rate and a higher occurrence of severe dysphagia and need for walking aid compared to PSP variants such as PSP Parkinsonism (PSP-P), PSP postural instability without ocular motor dysfunction (PSP-PI), and other rare phenotypes. Conclusions PSP-RS has a less favorable prognosis compared to PSP variants stratified according to the MDS-PSP classification. This classification could assist in selecting patients for clinical trials and help design outcomes that account for the disease heterogeneity.
International collaboration to improve knowledge on myotonic dystrophy type 2
Authors: Peric Stojan , Ivanovic Vukan , Ashley Emma-Jayne , Esparis Belen , Campbell Craig , Wenninger Stephan , Monckton Darren , Marini-Bettolo Chiara , Walker Helen , Voháňka Stanislav , Cumming Kleed , Łusakowska Anna , Hodgkinson Victoria , Cosyns Marjan , Rodrigues Miriam , Yiu Eppie , Mazanec Radim , Stevenson Tanya , Kostera-Pruszczyk Anna , Korngut Lawrence , Jagut Marlène , Schoser Benedikt , Forbes Robin , Poll Annie , Roxburgh Richard 
Published: December 2024

Background: The TREAT-NMD Global Registry Network is a global collaboration of neuromuscular disease registries, including myotonic dystrophy type 2 (DM2), which aims to facilitate collaborative research and clinical trials. Objectives: This study aimed to assess DM2 patients included in the network, and to analyse their socio-demographic and clinical features. Methods: Data were collected through email surveys sent to 16 TREAT-NMD myotonic dystrophy core member registries. 10 registries enrolled DM2 patients. Results: The total number of DM2 cases was 1,720, with the Czech, German, and USA registries enrolling the most patients (445, 430, and 339 cases, respectively). The highest rates were seen in Czechia and Serbia (4.2 and 2.0 registered per 100,000 population, respectively). High DM2:DM1 ratios were seen in Central Europe. The median age at registry entry was 51 years. Symptom onset occurred before age 20 in 14% of cases. One fifth of patients used an assistive device to walk, and 4% were non-ambulatory. Insertion of a pacemaker or implantable cardioverter-defibrillator was reported in 4% of subjects, while 7% used non-invasive ventilation. Conclusions: This represents the largest DM2 cohort assembled to date, providing demographic and clinical data for future research and trial recruitment, illustrating TREAT-NMD's international reach and the importance of capturing DM2 data.