bluuebunny/crossref_metadata_2025_split huggingface dataset:
IndigenousBacillus paramycoidesandAlcaligenes faecalis: potential solution for the bioremediation of wastewaters
Authors: Rashid Aneeba , Mirza Safdar A. , Keating Ciara , Ali Sikander , Campos Luiza C. 
Published: May 2020

AbstractFarmers near towns and cities are using wide range of untreated wastewaters for crop irrigation in Pakistan due to severe freshwater shortage. The present study aimed to treat different types of wastewater including domestic, hospital, textile, pharmaceutical and mixed wastewaters using indigenous bacterial isolates to remove contaminants and render these wastewaters safer for irrigation. 37 bacterial strains were isolated from the 5 wastewater samples collected from different sites in Lahore, Pakistan. Under optimum growth conditions, the isolates D6, D7 and P1 showed maximum decolourisation potential of 96, 96, 93 %, respectively against hospital wastewater. GCMS analysis of the untreated hospital wastewater confirmed the presence of pharmaceutic pollutants i.e. Phenol, Salicylic acid, Caffeine, Naproxen, Octadecene and Diazepam. These organic compounds were biodegraded into derivate Ticlopidine in the case of isolate D6, derivatives Tetradecene and Griseofulvin in the case of isolate D7, and derivatives Lidocaine and Butalbital in the case of isolate P1. 16S rDNA sequencing was used to identify these isolates. Isolates D6 and D7 showed 100 and 99.86 % homology toBacillus paramycoides, a novel strain fromBacillus cereusgroup (Liu et al., 2017). Isolate P1 showed 97.47 % homology toAlcaligenes faecalis. These strains therefore could represent a low-cost and low-tech alternative to bioremediate complex wastewaters prior to irrigation to support the achievement of the Sustainable Development Goal 6 - clean water and sanitation in Pakistan.
The calcium binding protein S100β marks hedgehog-responsive resident vascular stem cells within vascular lesions
Authors: Luca Mariana Di , Fitzpatrick Emma , Burtenshaw Denise , Liu Weimin , Helt Jay-Christian , Hakimjavadi Roya , Corcoran Eoin , Gusti Yusof , Sheridan Daniel , Harman Susan , Lally Catriona , Redmond Eileen M. , Cahill Paul A. 
Published: May 2020

AbstractA hallmark of subclinical atherosclerosis is the accumulation of vascular smooth muscle cell (SMC)-like cells leading to intimal thickening. While medial SMCs contribute, the participation of hedgehog responsive resident vascular stem cells (vSCs) to lesion formation remains unclear. Using transgenic eGFP mice and genetic lineage tracing of S100β vSCsin vivo, we identified S100β/Sca1 cells derived from a S100β non-SMC parent population within lesions that co-localise with smooth muscle ⍰-actin (SMA) cells following iatrogenic flow restriction, an effect attenuated following hedgehog inhibition with the smoothened inhibitor, cyclopamine.In vitro, S100β/Sca1 cells isolated from atheroprone regions of the mouse aorta expressed hedgehog signalling components, acquired the di-methylation of histone 3 lysine 4 (H3K4me2) stable SMC epigenetic mark at the Myh11 locus and underwent myogenic differentiation in response to recombinant sonic hedgehog (SHh). Both S100β and PTCH1 cells were present in human vessels while S100β cells were enriched in arteriosclerotic lesions. Recombinant SHh promoted myogenic differentiation of human induced pluripotent stem cell-derived S100β neuroectoderm progenitorsIn vitro. We conclude that hedgehog responsive S100β vSCs contribute to lesion formation and support targeting hedgehog signalling to treat subclinical arteriosclerosis.
Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signaling
Authors: Koning Jasper J. , Rajaraman Anusha , Reijmers Rogier M. , Konijn Tanja , Pan Junliang , Ware Carl F. , Butcher Eugene C. , Mebius Reina E. 
Published: May 2020

AbstractSpecialized stromal cells occupy and help define B- and T cell domains, which is crucial for proper functioning of our immune system. Signaling through lymphotoxin and TNF-receptors is crucial for development of different stromal subsets which are thought to arise from a common precursor. However, mechanisms that control the selective generation of the different stromal phenotypes are not known.Here we show that in mice, retinoic acid mediated signaling is important for the differentiation of precursors towards the Cxcl13posfollicular dendritic cell (FDC) lineage, while blocking lymphotoxin mediated Ccl19posfibroblastic reticular cell (FRC) lineage differentiation. Consequently, we see at day of birth Cxcl13posCcl19neg/lowand Cxcl13neg/lowCcl19poscells within neonatal lymph nodes.Furthermore, ablation of retinoic acid receptor signaling in stromal precursors early after birth reduces Cxcl13 expression, while in addition, complete blockade of retinoic acid signaling prevents formation of FDC networks in lymph nodes.
Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cells
Authors: Albanese Manuel , Chen Yen-Fu Adam , Hüls Corinna , Gärtner Kathrin , Tagawa Takanobu , Mejias-Perez Ernesto , Keppler Oliver T. , Göbel Christine , Zeidler Reinhard , Shein Mikhail , Schütz Anne K. , Hammerschmidt Wolfgang 
Published: May 2020

ABSTRACTMammalian cells release different types of vesicles, collectively termed extracellular vesicles (EVs). EVs contain cellular microRNAs (miRNAs) with an apparent potential to deliver their miRNA cargo to recipient cells to affect the stability of individual mRNAs and the cells’ transcriptome. The extent to which miRNAs are exported via the EV route and whether they contribute to cell-cell communication are controversial. To address these issues, we analyzed the capacity of EVs to deliver packaged miRNAs into target cells and to exert biological functions. We applied well-defined approaches to produce and characterize purified EVs with or without specific viral miRNAs. We found that only a small fraction of EVs carried miRNAs. EVs readily bound to different target cell types, but there was no EV-cell membrane fusion or delivery of cargo. Importantly, the functionality of cells exposed to miRNA-carrying EVs was not affected. These results suggest EV-borne miRNAs do not act as effectors and question their relevancy in paracrine cell-to-cell communication.AUTHOR SUMMARYThe majority of metazoan cells release vesicles of different types and origins, such as exosomes and microvesicles, now collectively termed extracellular vesicles (EVs). EVs have gained much attention because they contain microRNAs (miRNAs) and thus could regulate their specific mRNA targets in recipient or acceptor cells that take up EVs. Using a novel fusion assay with superior sensitivity and specificity, we revisited this claim but found no convincing evidence for an efficient functional uptake of EVs in many different cell lines and primary human blood cells. Even EVs engineered to fuse and deliver their miRNA cargo to recipient cells had no measurable effect on target mRNAs in very carefully controlled, quantitative experiments. Our negative results clearly indicate that EVs do not act as vehicles for miRNA-based cell-to-cell communication.
Resistance to serine inBacillus subtilis: Identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism
Authors: Klewing Anika , Koo Byoung Mo , Krüger Larissa , Poehlein Anja , Reuß Daniel , Daniel Rolf , Gross Carol A. , Stülke Jörg 
Published: May 2020

SummaryThe Gram-positive bacteriumBacillus subtilisuses serine not only as building block for proteins but also as an important precursor in many anabolic reactions. Moreover, a lack of serine results in the initiation of biofilm formation. However, in excess serine inhibits the growth ofB. subtilis. To unravel the underlying mechanisms, we isolated suppressor mutants that can tolerate toxic serine concentrations by three targeted and non-targeted genome-wide screens. All screens as well as genetic complementation inEscherichia coliidentified the so far uncharacterized permease YbeC as the major serine transporter ofB. subtilis. In addition to YbeC, the threonine transporters BcaP and YbxG make minor contributions to serine uptake. A strain lacking these three transporters was able to tolerate 100 mM serine whereas the wild type strain was already inhibited by 1 mM of the amino acid. The screen for serine-resistant mutants also identified mutations that result in increased serine degradation and in increased expression of threonine biosynthetic enzymes suggesting that serine toxicity results from interference with threonine biosynthesis.Originality-Significance StatementSerine is an important precursor for many biosynthetic reactions, and lack of this amino acid can induce biofilm formation inBacillus subtilis. However, serine is toxic for the growth ofB. subtilis. To understand the reason(s) for this toxicity and to identify the so far unknown serine transporter(s) of this bacterium, we performed exhaustive mutant screens to isolate serine-resistant mutants. This screen identified YbeC, the major serine transporter ofB. subtilis. Moreover, we observed an intimate link between serine and threonine metabolism that is responsible for serine toxicity by inhibiting threonine biosynthesis.
Silica particles trigger the production of exopolysaccharides in harsh environment plant growth-promoting rhizobacteria and increase their ability to enhance drought tolerance
Authors: Fetsiukh Anastasiia , Conrad Julian , Bergquist Jonas , Ayele Fantaye , Timmusk Salme 
Published: May 2020

ABSTRACTIn coming decades drought is expected to expand globally owing to increased evaporation and reduced rainfall. In order to reduce the vulnerability of agricultural systems we need to understand the crop plant growth environment. Understanding, predicting and controlling the rhizosphere has potential to harness plant microbe interactions, improve plant responses to environmental stress and mitigate effects of climate change. Our plant growth-promoting rhizobacteria (PGPR) are isolated from the natural laboratory ‘Evolution Canyon’ Israel (EC). The endophytic rhizobacteria from the wild progenitors of cereals have been co-habituated with their hosts for long periods of time. The study revealed that silica particles (SN) triggered the PGPR production of exopolysaccharides (EPS) containing D-glucuronate (D-GA). This leads to increased plant biomass accumulation in drought-stressed growth environments. The PGPR increased EPS content increases the water holding capacity (WHC) and osmotic pressure of the biofilm matrix. Light- and electron-microscopic studies show that in the presence of SN particles, bacterial morphology is changed, indicating that SNs are associated with significant reprogramming in bacteria.The results here show that the production of EPS containing D-GA is induced by SN treatment. The findings encourage formulation of cells considering microencapsulation with materials that ensure higher WHC and hyperosmolarity under field conditions. Our results illustrate the importance of considering natural soil nanoparticles in the application of PGPR. Osmotic pressure involvement of holobiont cohabitation is discussed.
CD8+ T cell cross-reactivity against SARS-CoV-2 conferred by other coronavirus strains and influenza virus
Authors: Lee Chloe H. , Pereira Pinho Mariana , Buckley Paul , Woodhouse Isaac , Ogg Graham , Simmons Alison , Napolitani Giorgio , Koohy Hashem 
Published: May 2020

AbstractWhile individuals infected with coronavirus disease 2019 (COVID-19) manifested a broad range in susceptibility and severity to the disease, the pre-existing immune memory of related pathogens can influence the disease outcome. Here, we investigated the potential extent of T cell cross-reactivity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be conferred by other coronaviruses and influenza virus, and generated a map of public and private predicted CD8+ T cell epitopes between coronaviruses. Moreover, to assess the potential risk of self-reactivity and/or diminished T cell response for peptides identical or highly similar to the host, we identified predicted epitopes with high sequence similarity with human proteome. Lastly, we compared predicted epitopes from coronaviruses with epitopes from influenza virus deposited in IEDB to support vaccine development against different virus strains. We believe the comprehensivein silicoprofile of private and public predicted epitopes across coronaviruses and influenza viruses will facilitate design of vaccines capable of protecting against various viral infections.
FGFR Signaling Coordinates the Balance Between Glycolysis and Fatty Acid β-Oxidation in Lymphatic Endothelial Cells
Authors: Song Hongyuan , Zhu Jie , Fang Longhou , Yu Pengchun 
Published: May 2020

AbstractThe balance between glycolysis and oxidative phosphorylation is believed to be critical for maintaining cellular bioenergetics, yet the regulation of such balance in lymphatic endothelial cells (LECs) remains unclear. Here we found that chemical inhibition of fibroblast growth factor receptor (FGFR) activity or knockdown of FGFR1, which has been shown to suppress glycolysis and consequently ATP production, induces substantial upregulation of fatty acid β-oxidation (FAO), but not glucose oxidation or glutamine oxidation in LECs. Mechanistically, blockade of FGFR-AKT signaling promotes the expression of CPT1A, a rate-limiting enzyme of FAO, in a PPARα-dependent manner. Metabolic analysis further showed that CPT1A depletion impairs ATP generation in FGFR1-deficient rather than wild-type LECs. This result suggests that FAO, which makes a minor contribution to cellular energy under normal conditions, can compensate for energy deficiency caused by FGFR inhibition. Consequently, CPT1A silencing potentiates the effect of FGFR1 knockdown on impeding LEC proliferation and migration. Collectively, our study identified an FGFR-regulated metabolic balance that is important for LEC growth.
Non-canonical cell death by reassortant reovirus
Authors: Stewart Roxana M. Rodríguez , Raghuram Vishnu , Berry Jameson T.L. , Mainou Bernardo A. 
Published: May 2020

AbstractTriple-negative breast cancer (TNBC) constitutes 12% of all breast cancer and is associated with worse prognosis compared to other subtypes of breast cancer. Current therapies are limited to cytotoxic chemotherapy, radiation, and surgery, leaving a need for targeted therapeutics to improve outcomes for TNBC patients. Mammalian orthoreovirus (reovirus) is a nonenveloped, segmented, dsRNA virus in theReoviridaefamily. Reovirus preferentially kills transformed cells and is in clinical trials to assess its efficacy against several types of cancer. We previously engineered a reassortant reovirus, r2Reovirus, that infects TNBC cells more efficiently and induces cell death with faster kinetics than parental reoviruses. In this study, we sought to understand the mechanisms by which r2Reovirus induces cell death in TNBC cells. We show that r2Reovirus infection of TNBC cells of a mesenchymal-stem like (MSL) lineage downregulates the MAPK/ERK pathway and induces non-conventional cell death that is caspase dependent, but caspase 3-independent. Infection of different MSL lineage TNBC cells with r2Reovirus results in caspase 3-dependent cell death. We map the enhanced oncolytic properties of r2Reovirus in TNBC to epistatic interactions between the Type 3 Dearing M2 gene segment and Type 1 Lang genes. These findings suggest that the genetic composition of the host cell impacts the mechanism of reovirus-induced cell death in TNBC. Together, our data show that understanding host and virus determinants of cell death can identify novel properties and interactions between host and viral gene products that can be exploited for the development of improved viral oncolytics.ImportanceTriple negative breast cancer (TNBC) is unresponsive to hormone therapies, leaving patients afflicted with this disease with limited treatment options. We previously engineered an oncolytic reovirus (r2Reovirus) with enhanced infective and cytotoxic properties in TNBC cells. However, how r2Reovirus promotes TNBC cell death is not known. In this study, we show that reassortant r2Reovirus can promote non-conventional caspase-dependent but caspase 3-independent cell death and that the mechanism of cell death depends on the genetic composition of the host cell. We also map the enhanced oncolytic properties of r2Reovirus in TNBC to interactions between a Type 3 M2 gene segment and Type 1 genes. Our data show that understanding the interplay between the host cell environment and the genetic composition of oncolytic viruses is crucial for the development of efficacious viral oncolytics.
Rapture-ready darters: choice of reference genome and genotyping method (whole-genome or sequence capture) influence population genomic inference inEtheostoma
Authors: Reid Brendan N. , Moran Rachel L. , Kopack Christopher J. , Fitzpatrick Sarah W. 
Published: May 2020

AbstractResearchers studying non-model organisms have an increasing number of methods available for generating genomic data. However, the applicability of different methods across species, as well as the effect of reference genome choice on population genomic inference, are still difficult to predict in many cases. We evaluated the impact of data type (whole-genome vs. reduced representation) and reference genome choice on data quality and on population genomic and phylogenomic inference across several species of darters (subfamily Etheostomatinae), a highly diverse radiation of freshwater fish. We generated a high-quality reference genome and developed a hybrid RADseq/sequence capture (Rapture) protocol for the Arkansas darter (Etheostoma cragini). Rapture data from 1900 individuals spanning four darter species showed recovery of most loci across darter species at high depth and consistent estimates of heterozygosity regardless of reference genome choice. Loci with baits spanning both sides of the restriction enzyme cut site performed especially well across species. For low-coverage whole-genome data, choice of reference genome affected read depth and inferred heterozygosity. For similar amounts of sequence data, Rapture performed better at identifying fine-scale genetic structure compared to whole-genome sequencing. Rapture loci also recovered an accurate phylogeny for the study species and demonstrated high phylogenetic informativeness across the evolutionary history of the genusEtheostoma. Low cost and high cross-species effectiveness regardless of reference genome suggest that Rapture and similar sequence capture methods may be worthwhile choices for studies of diverse species radiations.
A flexible planning methodology for product family assembly line based on improved NSGA_II
Authors: Wu Yongming , Zhao Xudong , Xu Yanxia , Chen Yuling 
Published: May 2020

Purpose
The product family assembly line (PFAL) is a mixed model-assembly line, which is widely used in mass customization and intelligent manufacturing. The purpose of this paper is to study the problem of PFAL, a flexible (evolution) planning method to respond to product evolution for PFAL, to focus on product data analysis and evolution planning method.

Design/methodology/approach
The evolution balancing model for PFAL is established and an improved NSGA_II (INSGA_II) is proposed. From the perspective of data analysis, dynamic characteristics of PFAL are researched and analyzed. Especially the tasks, which stability is considered, can be divided into a platform and individual task. In INSGA_II algorithm, a new density selection and a decoding method based on sorting algorithms are proposed to compensate for the lack of traditional algorithms.

Findings
The effectiveness and feasibility of the method are validated by an example of PFAL evolution planning for a family of similar mechanical products. The optimized efficiency is significantly improved using INSGA_II proposed in this paper and the evolution planning model proposed has a stronger ability to respond to product evolution, which maximizes business performance over an effective period of time.

Originality/value
The assembly line designers and managers in discrete manufacturing companies can obtain an optimal solution for PFAL planning through the evolution planning model and INSGA-II proposed in this paper. Then, this planning model and optimization method have been successfully applied in the production of small wheel loaders.
Building career capital: developing business leaders' career mobility
Authors: Brown Cathy , Hooley Tristram , Wond Tracey 
Published: May 2020

PurposeCareer theorists have been increasingly occupied with role transitions across organisations, neglecting role transitions undertaken within single organisations. By exploring in depth the aspects of career capital that role holders need to facilitate their own organisational role transition, this article builds upon career capital theory.Design/methodology/approachAdopting an interpretivist approach, this study explores the experiences of 36 business leaders who have undertaken a recent role transition within a UK construction business.FindingsThe article empirically characterises 24 career capital aspects, clustered into Knowing Self, Knowing How and Knowing Whom. It argues that these aspects are important to internal role transitions and compares them to mainstream career capital theory. In addition, the concepts of connecting, crossing and investing career capital are introduced to explain how career capital supports such transitions.Research limitations/implicationsThis study proposes a new career capital framework and refocuses debate on organisational careers. It is based on a single organisation, and it would be beneficial for future researchers to explore its applicability within other organisations.Practical implicationsThe article explores the implications of the new career capital framework for business leaders and organisational managers who wish to build individual and organisational career mobility.Originality/valueThis study proposes a new, empirically grounded, career capital theoretical framework particularly attending to organisational role transitions.
Analyzing the intention of the households to drop off mobile phones to the collection boxes: empirical study in Malaysia
Authors: Afroz Rafia , Muhibbullah Mohammad , Farhana Puteri , Morshed Mohammad Niaz 
Published: May 2020

PurposeTo achieve proper waste management, the disposal of electronic waste (e-waste) is one suitable method. Most developing countries, including Malaysia, are facing lack of e-waste recycling facilities and low household participation. Using a survey method using a questionnaire, this study aims to examine the intention of Malaysian households to drop-off their mobile phones to the nearest collection boxes (n= 600).Design/methodology/approachThis study expanded the theory of planned behavior by adding environmental awareness and knowledge. In addition, the cost of disposal and the convenience of the available disposal infrastructure were measured as two parts of the perceived behavioral control.FindingsThe results of this study show that environmental knowledge and awareness have a significant impact on attitudes toward recycling intention of the households. In addition, it was also found that the attitude and cost of disposal infrastructure is positively related to household intention.Originality/valueThese results show that if e-waste collection boxes are provided to the nearest community and e-waste management information is distributed, this will increase household participation in e-waste management.