bluuebunny/crossref_metadata_2025_split huggingface dataset:
High‐Resolution Proxy Records From Two Simultaneously Grown Stalagmites From Zoolithencave (Southeastern Germany) and their Potential for Palaeoclimate Reconstruction
Authors: Riechelmann Dana F. C. , Riechelmann Sylvia , Wassenburg Jasper A. , Fohlmeister Jens , Schöne Bernd R. , Jochum Klaus Peter , Richter Detlev K. , Scholz Denis 
Published: May 2020

AbstractTwo small annually laminated stalagmites from Zoolithencave (southeastern Germany) grew between CE 1821 and 1970 (Zoo‐rez‐1) and CE 1835 and 1970 (Zoo‐rez‐2), respectively. Trace element concentrations were determined by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA‐ICP‐MS). Samples for δ13C and δ18O analyses were micromilled on annual and subannual resolution. Soil and host rock samples were analyzed by X‐Ray Diffraction (XRD) and their elemental concentrations determined via inductively coupled plasma optical emission spectrometer (ICP‐OES). Trace element concentrations in the stalagmites show two groups in the principal component analyses: one with Mg, Ba, and Sr and another with Y, P, and Al, respectively. The second group reflects the content of detrital material. Increased weathering of soil minerals seems to have a strong influence on the silicate/carbonate weathering ratio controlling the variability of Mg, Ba, and Sr. Meteorological and Global Network of Isotopes in Precipitation (GNIP) station data were used to calculate the δ18O values of the drip water (infiltration‐weighted, mean annual, and the mean of the winter precipitation δ18O values) as well as the corresponding speleothem calcite. The δ18O values calculated by the infiltration‐weighted model show similar patterns and amplitudes as the measured δ18O values of the two stalagmites. This suggests that the δ18O values of speleothem calcite reflect the δ18O values of infiltration‐weighted annual precipitation, which zis related to mean annual temperature, resulting in a significant correlation between mean annual temperature and the measured δ18O values of stalagmite Zoo‐rez‐2. This relationship could potentially be used for quantitative climate reconstruction in the future by extending the time series back in time with further stalagmites from Zoolithencave.
On Parameterizing Soil Evaporation in a Direct Remote Sensing Model of ET: PT‐JPL
Authors: Marshall Michael , Tu Kevin , Andreo Veronica 
Published: May 2020

AbstractRemote sensing models that measure evapotranspiration directly from the Penman‐Monteith or Priestley‐Taylor equations typically estimate the soil evaporation component over large areas using coarse spatial resolution relative humidity (RH) from geospatial climate datasets. As a result, the models tend to underperform in dry areas at local scales where moisture status is not well represented by surrounding areas. Earth observation sensors that monitor large‐scale global dynamics (e.g., MODIS) afford comparable spatial coverage and temporal frequency, but at a higher spatial resolution than geospatial climate datasets. In this study, we compared soil evaporation parameterized with optical and thermal indices derived from MODIS to RH‐based soil evaporation as implemented in the Priestley Taylor‐Jet Propulsion Laboratory (PT‐JPL) model. We evaluated the parameterizations by subtracting PT‐JPL transpiration from observation‐based flux tower evapotranspiration in agricultural fields across the contiguous United States. We compared the apparent thermal inertia (ATI) index, land surface water index (LSWI), normalized difference water index (NDWI), and a new index derived from red and shortwave infrared bands (soil moisture divergence index [SMDI]). Relationships were significant at the 95% confidence band. LSWI and SMDI explained 18–33% of variance in 8‐day soil evaporation. This led to a 3–11% increase in explained ET variance. LSWI and SMDI tended to perform better at the irrigated sites than RH. LSWI and SMDI led to markedly better performance over other indices at a seasonal time step. L‐band microwave backscatter can penetrate clouds and can distinguish soil from canopy moisture content. We are presently fusing red‐SWIR‐RADAR to improve soil evaporation estimation.
Bayesian modeling reveals host genetics associated with rumen microbiota jointly influence methane emission in dairy cows
Authors: Zhang Qianqian , Difford Gareth , Sahana Goutam , Løvendahl Peter , Lassen Jan , Lund Mogens Sandø , Guldbrandtsen Bernt , Janss Luc 
Published: May 2020

AbstractReducing methane emissions from livestock production is of great importance for the sustainable management of the Earth’s environment. Rumen microbiota play an important role in producing biogenic methane. However, knowledge of how host genetics influences variation in ruminal microbiota and their joint effects on methane emission is limited. We analyzed data from 750 dairy cows, using a Bayesian model to simultaneously assess the impact of host genetics and microbiota on host methane emission. We estimated that host genetics and microbiota explained 24% and 7%, respectively, of variation in host methane levels. In this Bayesian model, one bacterial genus explained up to 1.6% of the total microbiota variance. Further analysis was performed by a mixed linear model to estimate variance explained by host genomics in abundances of microbial genera and operational taxonomic units (OTU). Highest estimates were observed for a bacterial OTU with 33%, for an archaeal OTU with 26%, and for a microbial genus with 41% heritability. However, after multiple testing correction for the number of genera and OTUs modeled, none of the effects remained significant. We also used a mixed linear model to test effects of individual host genetic markers on microbial genera and OTUs. In this analysis, genetic markers inside host genes ABS4 and DNAJC10 were found associated with microbiota composition. We show that a Bayesian model can be utilized to model complex structure and relationship between microbiota simultaneously and their interaction with host genetics on methane emission. The host genome explains a significant fraction of between-individual variation in microbial abundance. Individual microbial taxonomic groups each only explain a small amount of variation in methane emissions. The identification of genes and genetic markers suggests that it is possible to design strategies for breeding cows with desired microbiota composition associated with phenotypes.
The heterocyclic compound Tempol inhibits the growth of cancer cells by interfering with glutamine metabolism
Authors: Ye Shuangyan , Xu Pengfei , Huang Mengqiu , Chen Xi , Zeng Sisi , Wang Qianli , Chen Jianping , Li Keyi , Gao Wenwen , Liu Ruiyuan , Liu Jingxian , Shao Yihao , Zhang Hui , Xu Yang , Zhang Qianbing , Zhong Zhuo , Wei Zibo , Wang Jiale , Hao Bingtao , Huang Wenhua , Liu Qiuzhen 
Published: May 2020

AbstractTempol (4-hydroxy-2,2,6,6-Tetramethylpiperidine-1-oxyl, TPL), a nitroxide compound, inhibits proliferation and increases the vulnerability of cancer cells to apoptosis induced by cytotoxic agents. However, the molecular mechanism of TPL inhibiting cancer cell proliferation has not been fully understood. In this study, we evaluated the metabolic effect of TPL on cancer cells and explored its cancer therapeutic potential. Extracellular flow assays showed that TPL inhibited cellular basal and maximal oxygen consumption rates of mitochondrial. 13C metabolic flux analysis showed that TPL treatment had minimal effect on glycolysis. However, we found that TPL inhibits glutamine metabolism by interfering with the oxidative tricarboxylic acid cycle (TCA) process and reductive glutamine process. We found that the inhibitory effect of TPL on metabolism occurs mainly on the step from citrate to α-ketoglutarate or vice versa. We also found that activity of isocitrate dehydrogenase IDH1 and IDH2, the key enzymes in TCA, were inhibited by TPL treatment. In xenograft mouse model, TPL treatment reduced tumor growth by inhibiting cellular proliferation of xenograft tumors. Thus, we provided a mechanism of TPL inhibiting cancer cell proliferation by interfering with glutamine utilization that is important for survival and proliferation of cancer cells. The study may help the development of a therapeutic strategy of TPL combined with other anticancer medicines.
anlotinib alters tumor immune microenvironment by downregulating PD-L1 expression on vascular endothelial cells
Authors: Liu Shaochuan , Qin Tingting , Liu Zhujun , Wang Jing , Jia Yanan , Feng Yingfang , Gao Yuan , Li Kai 
Published: May 2020

AbstractAberrant vascular network is a hallmark of cancer. However, the role of vascular endothelial cells (VECs)-expressing PD-L1 in tumor immune microenvironment and antiangiogenic therapy remains unclear. In this study, we used the specimens of cancer patients for immunohistochemical staining to observe the number of PD-L1+ CD34+ VECs and infiltrated immune cells inside tumor specimens. Immunofluorescence staining and flow cytometry were performed to observe the infiltration of CD8+ T cells and FoxP3+ T cells in tumor tissues. Here, we found that PD-L1 expression on VECs determined CD8+ T cells’, FoxP3+ T cells’ infiltration, and the prognosis of patients with lung adenocarcinoma. Anlotinib downregulated PD-L1 expression on VECs through the inactivation of AKT pathway, thereby improving the ratio of CD8/FoxP3 inside tumor and remolding the immune microenvironment. In conclusion, our results demonstrate that PD-L1 high expression on VECs inhibits the infiltration of CD8+ T cells, whereas promotes the aggregation of FoxP3+ T cells into tumor tissues, thus becoming an “immunosuppressive barrier”. Anlotinib can ameliorate the immuno-microenvironment by downregulating PD-L1 expression on VECs to inhibit tumor growth.
Activation and targeting of ATG8 protein lipidation
Authors: Martens Sascha , Fracchiolla Dorotea 
Published: May 2020

AbstractATG8 family proteins are evolutionary conserved ubiquitin-like modifiers, which become attached to the headgroup of the membrane lipid phosphatidylethanolamine in a process referred to as lipidation. This reaction is carried out analogous to the conjugation of ubiquitin to its target proteins, involving the E1-like ATG7, the E2-like ATG3 and the E3-like ATG12–ATG5–ATG16 complex, which determines the site of lipidation. ATG8 lipidation is a hallmark of autophagy where these proteins are involved in autophagosome formation, the fusion of autophagosomes with lysosomes and cargo selection. However, it has become evident that ATG8 lipidation also occurs in processes that are not directly related to autophagy. Here we discuss recent insights into the targeting of ATG8 lipidation in autophagy and other pathways with special emphasis on the recruitment and activation of the E3-like complex.
Mendelian neurodegenerative disease genes involved in autophagy
Authors: Stamatakou Eleanna , Wróbel Lidia , Hill Sandra Malmgren , Puri Claudia , Son Sung Min , Fujimaki Motoki , Zhu Ye , Siddiqi Farah , Fernandez-Estevez Marian , Manni Marco M. , Park So Jung , Villeneuve Julien , Rubinsztein David Chaim 
Published: May 2020

AbstractThe lysosomal degradation pathway of macroautophagy (herein referred to as autophagy) plays a crucial role in cellular physiology by regulating the removal of unwanted cargoes such as protein aggregates and damaged organelles. Over the last five decades, significant progress has been made in understanding the molecular mechanisms that regulate autophagy and its roles in human physiology and diseases. These advances, together with discoveries in human genetics linking autophagy-related gene mutations to specific diseases, provide a better understanding of the mechanisms by which autophagy-dependent pathways can be potentially targeted for treating human diseases. Here, we review mutations that have been identified in genes involved in autophagy and their associations with neurodegenerative diseases.
Natural polyphenol assisted delivery of single-strand oligonucleotides by cationic polymers
Authors: Shen Wanwan , Wang Ruojun , Fan Qianqian , Li Yiwen , Cheng Yiyun 
Published: May 2020

AbstractSingle-strand oligonucleotides provide promising potential as new therapeutics towards various diseases. However, the efficient delivery of oligonucleotide therapeutics is still challenging due to their susceptibility to nuclease degradation and the lack of effective carriers for condensation. In this study, we reported the use of natural polyphenol to facilitate the condensation of single-strand oligonucleotides by cationic polymers. Green tea catechin complexed with single-strand oligonucleotides to form anionic nanoparticles, which were further coated by low molecular weight cationic polymers to increase their cell internalization. The resulting core-shell structured nanoparticles, so-called green nanoparticles (GNPs), showed improved cargo stability, and achieved high efficiency in the delivery of several types of single-strand oligonucleotides including antisense oligonucleotides, anti-miRNA, and DNAzyme. This study provides a facile strategy for the efficient delivery of single-strand oligonucleotides.
A human monoclonal antibody blocking SARS-CoV-2 infection
Authors: Wang Chunyan , Li Wentao , Drabek Dubravka , Okba Nisreen M. A. , van Haperen Rien , Osterhaus Albert D. M. E. , van Kuppeveld Frank J. M. , Haagmans Bart L. , Grosveld Frank , Bosch Berend-Jan 
Published: May 2020

AbstractThe emergence of the novel human coronavirus SARS-CoV-2 in Wuhan, China has caused a worldwide epidemic of respiratory disease (COVID-19). Vaccines and targeted therapeutics for treatment of this disease are currently lacking. Here we report a human monoclonal antibody that neutralizes SARS-CoV-2 (and SARS-CoV) in cell culture. This cross-neutralizing antibody targets a communal epitope on these viruses and may offer potential for prevention and treatment of COVID-19.
Challenges in returning results in a genomic medicine implementation study: the Return of Actionable Variants Empirical (RAVE) study
Authors: Kochan David C. , Winkler Erin , Lindor Noralane , Shaibi Gabriel Q. , Olson Janet , Caraballo Pedro J. , Freimuth Robert , Pacyna Joel E. , Breitkopf Carmen Radecki , Sharp Richard R. , Kullo Iftikhar J. 
Published: May 2020

AbstractTo inform the process of returning results in genome sequencing studies, we conducted a quantitative and qualitative assessment of challenges encountered during the Return of Actionable Variants Empiric (RAVE) study conducted at Mayo Clinic. Participants (n = 2535, mean age 63 ± 7, 57% female) were sequenced for 68 clinically actionable genes and 14 single nucleotide variants. Of 122 actionable results detected, 118 were returnable; results were returned by a genetic counselor—86 in-person and 12 by phone. Challenges in returning actionable results were encountered in a significant proportion (38%) of the cohort and were related to sequencing and participant contact. Sequencing related challenges (n = 14), affecting 13 participants, included reports revised based on clinical presentation (n = 3); reports requiring corrections (n = 2); mosaicism requiring alternative DNA samples for confirmation (n = 3); and variant re-interpretation due to updated informatics pipelines (n = 6). Participant contact related challenges (n = 44), affecting 38 participants, included nonresponders (n = 20), decedents (n = 1), and previously known results (n = 23). These results should be helpful to investigators preparing for return of results in large-scale genomic sequencing projects.
Pairing mechanism in multiband superconductors
Authors: Huang Wen-Min , Lin Hsiu-Hau 
Published: May 2020

AbstractIt has been a long-standing puzzle why electrons with repulsive interactions can form pairs in unconventional superconductors. Here we develop an analytic solution for renormalization group analysis in multiband superconductors, which agrees with the numerical results exceedingly well. The analytic solution allows us to construct soluble effective theory and answers the pairing puzzle: electrons form pairs resonating between different bands to compensate the energy penalty for bring them together, just like the resonating chemical bonds in benzene. The analytic solutions allow us to explain the peculiar features of critical temperatures, spin uctuations in unconventional superconductors and can be generalized to cuprates where the notion of multibands is replaced by multipatches in momentum space.
Snakes elicit specific neural responses in the human infant brain
Authors: Bertels J. , Bourguignon M. , de Heering A. , Chetail F. , De Tiège X. , Cleeremans A. , Destrebecqz A. 
Published: May 2020

AbstractDetecting predators is essential for survival. Given that snakes are the first of primates’ major predators, natural selection may have fostered efficient snake detection mechanisms to allow for optimal defensive behavior. Here, we provide electrophysiological evidence for a brain-anchored evolved predisposition to rapidly detect snakes in humans, which does not depend on previous exposure or knowledge about snakes. To do so, we recorded scalp electrical brain activity in 7- to 10-month-old infants watching sequences of flickering animal pictures. All animals were presented in their natural background. We showed that glancing at snakes generates specific neural responses in the infant brain, that are higher in amplitude than those generated by frogs or caterpillars, especially in the occipital region of the brain. The temporal dynamics of these neural responses support that infants devote increased attention to snakes than to non-snake stimuli. These results therefore demonstrate that a single fixation at snakes is sufficient to generate a prompt and large selective response in the infant brain. They argue for the existence in humans of an inborn, brain-anchored mechanism to swiftly detect snakes based on their characteristic visual features.
Differences in intracellular localisation of ANKH mutants that relate to mechanisms of calcium pyrophosphate deposition disease and craniometaphyseal dysplasia
Authors: Vijen Sunny , Hawes Chris , Runions John , Russell R. Graham G. , Wordsworth B. Paul , Carr Andrew J. , Pink Ryan C. , Zhang Yun 
Published: May 2020

AbstractANKH mutations are associated with calcium pyrophosphate deposition disease and craniometaphyseal dysplasia. This study investigated the effects of these ANKH mutants on cellular localisation and associated biochemistry. We generated four ANKH overexpression-plasmids containing either calcium pyrophosphate deposition disease or craniometaphyseal dysplasia linked mutations: P5L, E490del and S375del, G389R. They were transfected into CH-8 articular chondrocytes and HEK293 cells. The ANKH mutants dynamic differential localisations were imaged and we investigated the interactions with the autophagy marker LC3. Extracellular inorganic pyrophosphate, mineralization, ENPP1 activity expression of ENPP1, TNAP and PIT-1 were measured. P5L delayed cell membrane localisation but once recruited into the membrane it increased extracellular inorganic pyrophosphate, mineralization, and ENPP1 activity. E490del remained mostly cytoplasmic, forming punctate co-localisations with LC3, increased mineralization, ENPP1 and ENPP1 activity with an initial but unsustained increase in TNAP and PIT-1. S375del trended to decrease extracellular inorganic pyrophosphate, increase mineralization. G389R delayed cell membrane localisation, trended to decrease extracellular inorganic pyrophosphate, increased mineralization and co-localised with LC3. Our results demonstrate a link between pathological localisation of ANKH mutants with different degrees in mineralization. Furthermore, mutant ANKH functions are related to synthesis of defective proteins, inorganic pyrophosphate transport, ENPP1 activity and expression of ENPP1, TNAP and PIT-1.
Molecular insights and cell cycle assessment upon exposure to Chaga (Inonotus obliquus) mushroom polysaccharides in zebrafish (Danio rerio)
Authors: Eid Jehane Ibrahim , Das Biswadeep 
Published: May 2020

AbstractChaga (Inonotus obliquus) mushroom is considered as one of the most powerful antioxidants across the world. Though the therapeutic effects of Chaga components are well characterized in vitro, the in vivo developmental effects are not elucidated in detail. In this study, we assessed the in vivo developmental effects of Chaga polysaccharides in zebrafish, along with revealing the effects on cell cycle and apoptosis. Chaga mushroom polysaccharides comprised xylulose, rhamnose, mannose, glucose, inositol, and galactose, in addition to phenolic compounds; zebrafish embryos exhibited normal embryonic development upon transient exposure to Chaga extract (24 hours). Most embryos (>90%) were found to be healthy even at high concentrations (5 mg/mL). In addition, staining with the DNA binding dye, acridine orange showed that Chaga polysaccharides alleviated oxidative stress. Flow cytometric analysis using H2DCFDA that specifically binds to cells with fragmented DNA showed significantly reduced levels of intracellular reactive oxygen species (ROS) (p < 0.05), which in turn reduced apoptosis in the developing embryos. Cell cycle analysis by measuring the DNA content using flow cytometry revealed that Chaga polysaccharides moderately arrested the cells at G1 stage, thereby inhibiting cell proliferation that can be further explored in cancer studies. Overall, transient exposure of Chaga polysaccharide extract reduced intracellular ROS and assisted in the normal development of zebrafish.
On the interchangeability of sea-surface and near-surface air temperature anomalies in climatologies
Authors: Rubino Angelo , Zanchettin Davide , De Rovere Francesco , McPhaden Michael J. 
Published: May 2020

AbstractOn global and hemispheric scales, sea-surface temperature (SST) anomalies are assumed to be good surrogates for near-surface marine air temperature (MAT) anomalies. In fact, global gridded temperature datasets commonly blend SST and near-surface air temperature anomalies to overcome the lack of geographically homogeneous and reliable MAT observations. Here, we show that SST and MAT anomalies differ regarding crucial statistical properties such as multiannual trends and probabilistic distributions of daily and monthly averages. We provide evidence of the lack of interchangeability from an array of moored buoys in the tropical Pacific Ocean. We identify statistically significant discrepancies between SST and MAT anomalies for single as well as groups of such buoys. Thus, caution is required when characterizing and interpreting MAT variability through SST observations, especially at shorter than decadal timescale.