bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety and efficacy of a novel cephalomedullary nail in femoral shaft fractures: a retrospective observational cohort in 33 patients
Authors: De Leon Jorge Clint , Tye Cooper Benjamin , Breinholt Connor Scott , Dang Khang H , Karia Ravi A. 
Published: October 2020

Abstract
Background: Despite advances in femoral shaft fracture fixation, the nonunion rate remains relatively high; and there is limited data on the efficacy and failure rate of specific implants. A novel cephalomedullary nail that provides the ability to treat femur shaft fractures in isolation, with associated ipsilateral femur injuries, and provides various options for proximal and distal fixation exists on the market; but little is available on the safety and efficacy of this implant. The aim of this study is to evaluate the early failure rate of this cephalomedullary nail, while comparing the nonunion rate to what is currently presented in the literature. This study is the first of its kind in evaluation of a specific implant for treatment of femoral shaft fractures and ipsilateral pathology.Methods: Patients over 18 years of age, with traumatic femur shaft fractures, treated with this novel cephalomedullary nail and available for a minimum of 3-month follow-up were included for analysis. Retrospective chart review for this retrospective observational cohort study was performed. Demographic data, injury details, AO/OTA fracture classification, and implant details were recorded for each patient. Primary outcome measured was implant failures (screw or nail breakage). Secondary outcomes measured included malunion, nonunion, deep infection, post-operative complications, and need for reoperation. We hypothesize that the union and failure rate will be similar to that reported in the literature for femur shaft fractures.Results: Of the 33 patients included for analysis, 1 patient went on to non-union. There were no cases of implant failure. The single nonunion was a high-energy mechanism, open fracture, and higher level AO/OTA classification. The remaining 32 reached radiographic union at 3 months. Conclusion: The nonunion rate of this novel cephalomedullary nail is comparable to what is reported in the literature. This nail is a safe and effective implant to treat femoral shaft fractures with a variety of ipsilateral femoral shaft injuries and reliably leads fracture union. Further studies are needed analyzing implant failure and comparing specific implants.
Construction of a high density genetic map and QTL mapping of PEG-induced drought tolerance at the early seedling stage in sesame using whole genome re-sequencing
Authors: Liang Junchao , Ye Yanying , Yan Xiaowen , Yan Tingxian , Rao Yueliang , Zhou Hongying , Sun Jian , Le Meiwang 
Published: October 2020

Abstract
BackgroundImprovement in sesame (Sesamum indicum L.) drought tolerance at seedling stage is important for yield stability. Genetic approaches combing with conventional breeding is the most effective way to develop drought-tolerant cultivars. So far, only a few studies have been reported to reveal gene/ quantitative trait loci (QTL) controlling drought tolerance in sesame. To identify the genomic regions associated with drought tolerance, we constructed a high-density genetic map using a recombinant inbred line (RIL) population through whole genome re-sequencing (WGRS) technique. QTLs contributing to three seedling traits were identified under both non-stress and water stress conditions.ResultsThree drought tolerance related traits and their relative values (the ratio of value under stress to value under control condition), including seedling weight (SW), shoot length (SL) and root length (RL), were evaluated under control and PEG-induced osmotic conditions at seedling stage in a RIL population derived from cross of Zhushanbai (ZSB) and Jinhuangma (JHM). Significant variation and high broad sense heritability were observed for all traits except SW under stress condition in the population. With this population, a high-density linkage map with 1354 bin markers was constructed through WGRS strategy. Composite interval mapping analysis was performed for all the traits as well as their relative phenotypic data. A total of 34 QTLs were detected for these traits under both conditions and their relative values, and 13 stable QTLs associated with seven traits were revealed in two independent experiments, explaining on average, 4.95-16.26% of phenotypic variation for each QTL. Four of them contributed more than 10% of phenotypic variation. One region on chromosome 12 contained two major QTLs related to RL under osmotic condition and relative RL. Seven candidate genes underlying major QTLs for drought tolerance were identified according to gene descriptions and variations between parents.ConclusionThe current study reports the first QTL mapping of drought tolerance related traits through a RIL population and first QTL detection of root related trait (root length) in sesame. These findings will provide new genetic resources for molecular improvement of drought tolerance and candidate gene identification in sesame.
Strength and Size of Phosphorus-Rich Patches Determine the Foraging Strategy of Neyraudia reynaudiana
Authors: Cai Liping , Wang Yuzhen , Tigabu Mulualem , Hou Xiaolong , Wu Pengfei , Zhou Chuifan , Ma Xiangqing , Cai Liping 
Published: October 2020

Abstract
Background: Under natural conditions, soil nutrients are heterogeneously distributed, and plants have developed adaptation strategies, such as root morphological plasticity and/or physiological plasticity, to efficiently forage patchily distributed nutrient. Most previous studies examined either patch strength or patch size separately and focused mainly on root morphological plasticity, thus the effects of both patch strength and size on morphological and physiological plasticity are not well understood. In this study, we examined the foraging strategy of Neyraudia reynaudiana (Kunth) Keng ex Hithc, a pioneer grass colonizing degraded sites, with respect to patch strength and size in heterogeneously distributed phosphorus (P), and how foraging patchily distributed P affects total plant biomass. Plants were grown in sand-culture pots divided into ½, ¼, 1/6 compartments and full size and supplied with 0 + 0/30, 0 + 7.5/30 and 7.5 + 0/30 mg P/kg dry soil as KH2PO4 or 0 + 15/15, 0 + 18.5/ 18.5, 7.5 + 15/15 mg kg−1 in the homogenous treatment. The first amount was the P concentration in the central region, and that the second amount was the P concentration in the outer parts of the pot.Results: After 3 months of growth under experimental conditions, significantly (p < 0.05) high root elongation, root surface area, root volume and average root diameter was observed in large patches with high patch strength. Roots absorbed significantly more P in P-replete than P-deficient patches. Whole plant biomass was significantly higher in larger patches with high patch strength than small patches and homogeneous P distribution.Conclusion: The result demonstrates that root morphological and physiological plasticity are important adaptive strategies for foraging patchily distributed P and the former is largely determined by patch strength and size. The results also establish that foraging patchily distributed P resulted in increased total plant biomass compared to homogeneous P distribution.
Effect of APOE ε4 genotype on amyloid-β and tau accumulation in Alzheimer’s disease
Authors: Baek Min Seok , Cho Hanna , Lee Hye Sun , Lee Jae Hoon , Ryu Young Hoon , Lyoo Chul Hyoung 
Published: October 2020

Abstract
Background: To assess the effects of apolipoprotein E (ApoE) ε4 genotype on amyloid-β (Aβ) and tau burden and their longitudinal changes in Alzheimer’s disease (AD) spectrum.Methods: Among 272 individuals who underwent PET scans (18F-florbetaben for Aβ and 18F-flortaucipir for tau) and ApoE genotyping, 187 individuals completed 2-year follow-up PET scans. After correcting for the partial-volume effect, we compared the standardized uptake value ratio (SUVR) for Aβ and tau burden between the ε4+ and ε4- groups. By using a linear mixed-effect model, we measured changes in SUVR in the ApoE ε4+ and ε4- groups.Results: The ε4+ group showed greater baseline Aβ burden in the diffuse cortical regions and greater tau burden in the lateral, and medial temporal, cingulate, and insula cortices. Tau accumulation rate was higher in the parietal, occipital, lateral, and medial temporal cortices in the ε4+ group. In Aβ+ individuals, baseline tau burden was greater in the medial temporal cortex, while Aβ burden was conversely greater in the ε4- group. Tau accumulation rate was higher in the ε4+ group in a small region in the lateral temporal cortex. The effect of ApoE ε4 on enhanced tau accumulation persisted even after adjusting for the global cortical Aβ burden.Conclusions: Progressive tau accumulation may be more prominent in ε4 carriers, particularly in the medial and lateral temporal cortices. ApoE ε4 allele has differential effects on the Aβ burden depending on the existing amyloidosis and may enhance vulnerability to progressive tau accumulation in the AD spectrum independent of Aβ.
Age Distribution and Associated Factors of Cornea Biomechanical Parameter Stress-Strain Index in Chinese Healthy Population
Authors: Liu Guihua , Rong Hua , Pei Ruxia , Du Bei , Jin Nan , Wang Di , Jin Chengcheng , Wei Ruihua 
Published: October 2020

Abstract
Background: To investigate the new cornea Biomechanical parameter stress-strain index (SSI) in Chinese healthy people and the factors associated with SSI. Methods: A total of 175 eyes from 175 participants were included in this study. Axial length was measured with the Lenstar LS-900. Pentacam measured curvature of the cornea and anterior chamber volume (ACV). Cornea biomechanical properties assessments were performed by corneal visualization Scheimpflug technology (Corvis ST). Student’s t-test, one-way ANOVA, partial least square linear regression (PLSLR) and linear mixed effects (LME) model were used in the statistical analysis. Results: The mean (±SD) SSI was 1.14 ± 0.22 (range, 0.66–1.78) in all subjects and affected by age significantly after age of 35 (P < 0.05). In LME models, SSI was significantly associated with age (β=0.526, P<0.001), axial length (AL) (β=-0.541, P<0.001), intraocular pressure (IOP) (β=0.326, P<0.001) and steepest radius of anterior corneal curvature (RsF) (β=0.229, P<0.001) but not with ACV, biomechanical corrected intraocular pressure (bIOP), flattest radius of anterior corneal curvature (RfF) or central corneal thickness (CCT) (P>0.05 for each). Conclusions: SSI increased with age after the age of 35. In addition to age, SSI was positively correlated with RsF and IOP, while negatively correlated with AL.
Frequency of Fabry disease in a Juvenile Idiopathic Arthritis Cohort
Authors: Paim-Marques Luciana , Cavalcante Amanda Virginia , Verçosa Islane , Carneiro Paula , Souto-Maior Marcia , Marques Erlane , Appenzeller Simone 
Published: October 2020

Abstract
Background: Fabry disease (FD) is a rare, X-linked, multisystemic lysosomal storage disorder (LSD) that results from a deficiency in the hydrolase alpha-galactosidase A (⍺-GalA). During childhood, classic FD symptomatology is rare. The majority of children may show non-specific symptoms, including in the musculoskeletal system. The prevalence of FD among juvenile idiopathic arthritis (JIA) patients is unknown. Objective: The aim of this study was to identify the frequency of FD in a JIA cohort, characterizing early clinical symptoms, enzyme titers and GLA genotyping. Methods: Children with JIA followed in a tertiary Children Hospital cohort were selected. Clinical, laboratorial, and familiar information were recorded. Molecular genetic testing to detect GLA gene mutations was performed in girls and enzymatic analysis in boys. Results: In 89 patients (56.2% female, age at disease onset: 8.93 ± 4.35 years), one male (1.12%) patient presented pathogenic mutation in GLA gene, c.1244T>C p.L415P, one female patient had a variant of uncertain significance c.38C>T (p.Ala13Val). The enzymatic activity of alpha galactosidase was slightly decreased in 3 additional (3.4%) patients. We observed the presence of intronic variants in 44.44% patients in our cohort: c.1000-22C> T; c.370-81_-77del; c.640-16A> G; c.10C>T; c.548-125C> G and c.-12G> A. These variants and their combination were associated with clinical symptoms in our cohort.Conclusions: The incidence of FD in our cohort was 1.12%. Intronic variants were associated with symptoms previously described in literature. Screening for FD in JIA may be a reasonable strategy for those with atypical pattern of pain.
A Qualitative Study of Systemic Barriers Encountered by HIV-Positive Immigrants in Spain
Authors: Gogishvili Megi , Flórez Karen , Costa Sergio A. , Huang Terry T.-K. 
Published: October 2020

Abstract
Background: Immigrants are disproportionally impacted by HIV infection in Europe and in Spain. Immigrants are also identified as a vulnerable population during economic crises. Various socioeconomic barriers hinder HIV-positive immigrants from accessing healthcare services in the host country. As a result of the 2008 financial crisis, Spain has implemented multiple austerity measures, one of which was the enactments of Royal Decree Law (RDL) 16/2012 and Royal Decree (RD) 1192/2012 which abolished universal healthcare coverage. In this context, this study examined systemic barriers encountered by the participants while accessing health care after the enactments of 2012 RDL and RD. The study also researched distress felt by the participants and their experiences as HIV-positive immigrants living in Spain. Methods: Participants were recruited through a nongovernmental organization (NGO) during routine visits at the their center. A total of 12 participants were interviewed to reach data saturation. Participants were HIV-positive immigrants living in Spain for 1 or more years, allowing for substantial experience with navigating the healthcare system. Thematic analysis was performed to identify common themes in participants’ experiences living as HIV-positive individuals in Spain and confronting systemic barriers to healthcare access. Results: Four primary themes were identified. Participants identified experiencing emotional or physical (eg, side effects of medication) distress in adapting to life as HIV-positive individuals. Participants also expressed experiencing discrimination while living as HIV-positive immigrants in Spain. The primary systemic barrier to accessing health care encountered by participants was the inability to fulfill the requirement of having proof of registration in an Autonomous Community for the required time period, thus not being able to apply for a public health insurance card and utilize free care services. Participants identified a positive impact of third party (NGO, social worker, friend/family member) guidance on their experience of applying for a public health insurance card.Conclusions: HIV-positive immigrants are underserved in Spain. They encounter systemic barriers while accessing healthcare services, and experience fear and/or discrimination. The study underscores the role of NGOs in helping HIV-positive immigrants navigate the healthcare system. More research is needed on comprehensive approaches to address healthcare needs of HIV-positive immigrants in Spain.
Interventions to prevent anastomotic leak after esophageal surgery: A systematic review and meta-analysis
Authors: Grigor Emma J.M. , Kaaki Suha , Fergusson Dean A. , Maziak Donna E. , Seely Andrew J.E. 
Published: October 2020

Abstract
Background: Anastomotic leakage (AL) is a common and serious complication following esophagectomy. We aimed to provide an up-to-date review and critical appraisal of interventions designed to reduce AL risk. Methods: We searched MEDLINE and Embase from 1946 to January 2019 for randomized controlled trials (RCTs) evaluating interventions to minimize esophagogastric AL. Pooled risk ratios (RR) for AL was performed using random effects. Results: Two reviewers screened 441 abstracts and identified 17 RCTs eligible for inclusion; 11 studies were meta-analyzed. Omentoplasty reduced the risk of AL significantly by 78% [RR: 0.22; 95% CI: 0.10, 0.50] compared to no omentoplasty (3 studies, n = 611 patients). Early removal of NG tube reduced AL risk significantly by 62% [RR: 0.38; 95% CI: 0.02, 0.65] compared to prolonged NG tube (2 studies, n = 293 patients); Stapled (vs. hand-sewn) anastomosis did not significantly reduce AL risk [RR: 0.92; 95% CI: 0.45, 1.87] compared to hand-sewn (6 studies, n = 1,454 patients). The quality of evidence was high for omentoplasty (vs. no omentoplasty), moderate for early removal of NG tube (vs. conventional removal), and very low for stapled anastomosis (vs. hand-sewn). Conclusions: This is the first meta-analysis to summarize the graded quality of evidence for all RCT interventions designed to reduce AL following esophagectomy. Our findings demonstrated that omentoplasty reduced the risk of AL with a high quality of evidence. Although early nasogastric tube removal reduced AL risk, there is a need for further research to strengthen the quality of evidence. Evidence profiles presented in our review may help inform the development of clinical practice recommendations. Systematic review registration: CRD42019127181
Warming up Cooling Cooperators
Authors: Ferguson Eamonn , Lawrence Claire , Gemelli Carley , Rozsa Amy , Niekrasz Konrad , Masser Barbara , Davison Tanya 
Published: October 2020

Abstract
The warm-glow of giving is one mechanism proposed to account for high levels of human cooperation. However, little is known about how warm-glow can be harnessed to sustain long-term cooperation to benefit wider society (vaccinations, blood donation). We argue that the power of warm-glow to sustain long-term cooperation cools offs over time but can be warmed-up with a simple intervention message. We test, and confirm this prediction, in a field-based experiment (n = 5,821) comparing warm-glow versus positive affect messages to predict long-term cooperation (blood donation) and confirm our findings in a subsequent implementation study comparing donation attendance in a 3 year pre-implementation period, prior to the warm-glow message being sent to all 1st time donors in Australia (Ns = 90,317, 93,430 & 89,606) to a 2 year post-implementation period (Ns = 81,766 & 88,551). Exogenously manipulated warm-glow can encourage long-term cooperation that benefits society. Wider societal implications are discussed.
A Multi-Tier Approach to Estimate Energy Efficiency in Urban Passenger Mobility
Authors: Gonçalves Daniel Neves Schmitz , Bandeira Renata Albergaria de Mello , Assis Tássia Faria de , D'Agosto Marcio Almeida , Goes George Vasconcelos , Almeida Isabela Rocha Pombo Lessi de , Costa Mariane Gonzales da , Freitas Rodrigo Rodrigues de 
Published: October 2020

Abstract
Background: Recent studies evidence a lack of accurate methods to estimate energy efficiency in urban areas. This is due to the complex nature of obtaining wide range of activity and energy data from a single municipality, especially from developing countries, where data is usually scarce. Under these circumstances, this paper develops a method for estimating the energy efficiency in urban passenger mobility, considering three different levels of detail. The innovative factor is the use of a multi-tier approach to compare and adjust outputs. The method was applied in Sorocaba, Brazil, estimating a baseline of energy efficiency in this city. Results: Results show that energy efficiency varied from 0.67 passenger per kilometer/Mega Joule in 2013 to 0.70 passenger per kilometer /Mega Joule in 2017, which are consistent with the Brazilian passenger transport energy efficiency. Conclusions: The method proved to be an important mechanism for benchmarking purposes and for the decision-making process on transport investments. Moreover, it can be applied in cities from countries with different cultural and economic contexts.
Dog Breeds and Body Conformations with Predisposition to Osteosarcoma in the UK: A Case-Control Study
Authors: Edmunds Grace Louise , Smalley Matthew J , Beck Sam , Errington Rachel J , Gould Sara , Winter Helen , Brodbelt David C , O'Neill Dan G 
Published: October 2020

Abstract
BackgroundOsteosarcoma is an aggressive and painful bone neoplasm in dogs. Previous studies have reported epidemiological associations suggesting that large body mass, long bone length and the genetics of certain breeds including the Rottweiler are associated with elevated osteosarcoma risk. However, these studies were often limited by selection bias and confounding, and have rarely offered insights into breed-associated protection as well as breed-associated predisposition for osteosarcoma. The current study includes 1756 osteosarcoma cases presenting to VPG Histology (Bristol, UK) that are compared against a control population of 905,211 dogs without osteosarcoma taken from primary care electronic patient records in the VetCompass™ dataset. Methods and Study DesignRetrospective, case-control study. Multivariable logistic regression analysis explored associations between demographic risk factors (including breed, chondrodystrophy, age, sex/neuter status, skull-shape, and body mass) and osteosarcoma. ResultsWe identified several breeds with increased and reduced odds of osteosarcoma. At highest risk were the Rottweiler and Great Dane, with >10 times the odds of osteosarcoma compared with crossbreeds, and the Rhodesian Ridgeback; which has not featured in previous lists of at-risk breeds for osteosarcoma, and had an odds ratio of 11.31 (95% confidence interval 7.37 - 17.35). Breeds at lowest risk of osteosarcoma (protected breeds) included the Bichon Frise, the French Bulldog and the Cavalier King Charles Spaniel, all with odd ratios of less than 0.30 compared with crossbreeds. Body mass was strongly associated with osteosarcoma risk; dogs over 40kg exhibited osteosarcoma odds of 45.44 (95% confidence interval 33.74 - 61.20) compared with dogs less than 10kg. Chondrodystrophic breeds had an osteosarcoma odds ratio of 0.13 (95% confidence interval 0.11 - 0.16) compared with non-chondrodystrophic breeds. ConclusionsOverall, this study provides evidence of strong breed-associated osteosarcoma risk and protection, suggesting a genetic basis for osteosarcoma pathogenesis. The study findings highlight that breeds bred for long legs or large body mass are generally overrepresented amongst at-risk breeds, whilst those bred for short leg length or small body mass are generally protected. These findings could inform genetic studies to identify osteosarcoma risk alleles in canines and humans; as well as increasing awareness amongst vets and owners, resulting in improved breeding practices and clinical management of osteosarcoma in dogs.