bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultra-broadband local active noise control with remote acoustic sensing
Authors: Xiao Tong , Qiu Xiaojun , Halkon Benjamin 
Published: November 2020

AbstractOne enduring challenge for controlling high frequency sound in local active noise control (ANC) systems is to obtain the acoustic signal at the specific location to be controlled. In some applications such as in ANC headrest systems, it is not practical to install error microphones in a person’s ears to provide the user a quiet or optimally acoustically controlled environment. Many virtual error sensing approaches have been proposed to estimate the acoustic signal remotely with the current state-of-the-art method using an array of four microphones and a head tracking system to yield sound reduction up to 1 kHz for a single sound source. In the work reported in this paper, a novel approach of incorporating remote acoustic sensing using a laser Doppler vibrometer into an ANC headrest system is investigated. In this “virtual ANC headphone” system, a lightweight retro-reflective membrane pick-up is mounted in each synthetic ear of a head and torso simulator to determine the sound in the ear in real-time with minimal invasiveness. The membrane design and the effects of its location on the system performance are explored, the noise spectra in the ears without and with ANC for a variety of relevant primary sound fields are reported, and the performance of the system during head movements is demonstrated. The test results show that at least 10 dB sound attenuation can be realised in the ears over an extended frequency range (from 500 Hz to 6 kHz) under a complex sound field and for several common types of synthesised environmental noise, even in the presence of head motion.
Amoeba-inspired analog electronic computing system integrating resistance crossbar for solving the travelling salesman problem
Authors: Saito Kenta , Aono Masashi , Kasai Seiya 
Published: November 2020

AbstractCombinatorial optimization to search for the best solution across a vast number of legal candidates requires the development of a domain-specific computing architecture that can exploit the computational power of physical processes, as conventional general-purpose computers are not powerful enough. Recently, Ising machines that execute quantum annealing or related mechanisms for rapid search have attracted attention. These machines, however, are hard to map application problems into their architecture, and often converge even at an illegal candidate. Here, we demonstrate an analogue electronic computing system for solving the travelling salesman problem, which mimics efficient foraging behaviour of an amoeboid organism by the spontaneous dynamics of an electric current in its core and enables a high problem-mapping flexibility and resilience using a resistance crossbar circuit. The system has high application potential, as it can determine a high-quality legal solution in a time that grows proportionally to the problem size without suffering from the weaknesses of Ising machines.
Strength and microstructure properties of solidified sewage sludge with two types of cement-based binders
Authors: Chen Yijun , He Xingxing , Zhang Shaohua , Tan Xun , Wan Yong 
Published: November 2020

AbstractSolidification treatment with cementitious binder is an effective way to reduce environmental hazards of sewage sludge. Two cementitious binders, i.e., ordinary Portland cement (OPC) and sulfo-aluminate cement (SAC), were compared in this study to treat the sewage sludge. The strength of solidified sewage sludge (SSS) and changes in microscopic characteristics before and after treatment were analyzed through microscopic analysis methods. The effect of organic matter in sludge on the strength of SSS were also discussed. The results showed that the strength of SSS were lower than that of the solidified clay with no organic matter, and the filtrate extracted from the sludge can also weaken the cementation of the two cements significantly. The solidification effect of the OPC on the sludge was lower than that of the SAC evidently. The organic matter in the sewage sludge caused the surface of the soil particles to carry a large negative potential, which interfered with the hydration of the binder and reduced the amount of cementation skeleton formed by the binder hydration products. This resulted in a porous structure with low mechanical strength. The amount of early hydration product formed in the SAC-based solidified samples was higher than that of the OPC-based samples. This was favorable for filling the pores of the solidified samples and increasing their density. SAC had a better compatibility with soft soil containing high organic matter than OPC, and the which provides an effective alternative binder for dealing with sewage sludge.
Transcriptomic analysis of tobacco-flavored E-cigarette and menthol-flavored E-cigarette exposure in the human middle ear
Authors: Song Jae-Jun , Go Yoon Young , Lee Jong Kyou , Lee Bum Sang , Park Su-Kyoung , Jung Harry , Lee Jun Ho , Chang Jiwon 
Published: November 2020

AbstractElectronic cigarettes (e-cigarettes) are the most widely used electronic nicotine delivery systems and are designed to imitate smoking and aid in smoking cessation. Although the number of e-cigarette users is increasing rapidly, especially among young adults and adolescents, the potential health impacts and biologic effects of e-cigarettes still need to be elucidated. Our previous study demonstrated the cytotoxic effects of electronic liquids (e-liquids) in a human middle ear epithelial cell (HMEEC-1) line, which were affected by the manufacturer and flavoring agents regardless of the presence of nicotine. In this study, we aimed to evaluate the gene expression profile and identify potential molecular modulator genes and pathways in HMEEC-1 exposed to two different e-liquids (tobacco- and menthol-flavored). HMEEC-1 was exposed to e-liquids, and RNA sequencing, functional analysis, and pathway analysis were conducted to identify the resultant transcriptomic changes. A total of 843 genes were differentially expressed following exposure to the tobacco-flavored e-liquid, among which 262 genes were upregulated and 581 were downregulated. Upon exposure to the menthol-flavored e-liquid, a total of 589 genes were differentially expressed, among which 228 genes were upregulated and 361 were downregulated. Among the signaling pathways associated with the differentially expressed genes mediated by tobacco-flavored e-liquid exposure, several key molecular genes were identified, including IL6 (interleukin 6), PTGS2 (prostaglandin-endoperoxide synthase 2), CXCL8 (C-X-C motif chemokine ligand 8), JUN (Jun proto-oncogene), FOS (Fos proto-oncogene), and TP53 (tumor protein 53). Under menthol-flavored e-liquid treatment, MMP9 (matrix metallopeptidase 9), PTGS2 (prostaglandin-endoperoxide synthase 2), MYC (MYC proto-oncogene, bHLH transcription factor), HMOX1 (heme oxygenase 1), NOS3 (nitric oxide synthase 3), and CAV1 (caveolin 1) were predicted as key genes. In addition, we identified related cellular processes, including inflammatory responses, oxidative stress and carcinogenesis, under exposure to tobacco- and menthol-flavored e-liquids. We identified differentially expressed genes and related cellular processes and gene signaling pathways after e-cigarette exposure in human middle ear cells. These findings may provide useful evidence for understanding the effect of e-cigarette exposure.
Echocardiographic signs of subclinical cardiac function impairment in Duchenne dystrophy gene carriers
Authors: Kincl Vladimír , Panovský Roman , Pešl Martin , Máchal Jan , Juříková Lenka , Haberlová Jana , Masárová Lucia 
Published: November 2020

AbstractTo assess subclinical cardiac function impairment in Duchenne dystrophy (DMD) female carriers. Forty-four female subjects proved as DMD carriers underwent echocardiographic examination including tissue Doppler imaging (TDI) of mitral and tricuspid annulus. Seventeen age-matched healthy female subjects served as controls. A significant differences in peak systolic annular velocity (Sa) between carriers and controls were found for lateral and septal part of the mitral annulus and for tricuspid annulus (0.09 vs. 0.11 m/s, p < 0.001, 0.08 vs. 0.09 m/s, p < 0.01 and 0.13 vs. 0.14 m/s, p = 0.02 respectively). There was also difference in early diastolic velocity (Ea) of the septal part of the mitral annulus (0.11 vs. 0.13 m/s, p = 0.03). The subclinical deterioration of systolic function is presented even in asymptomatic DMD female carriers.
Type I toxin-antitoxin systems contribute to the maintenance of mobile genetic elements in Clostridioides difficile
Authors: Peltier Johann , Hamiot Audrey , Garneau Julian R. , Boudry Pierre , Maikova Anna , Hajnsdorf Eliane , Fortier Louis-Charles , Dupuy Bruno , Soutourina Olga 
Published: November 2020

AbstractToxin-antitoxin (TA) systems are widespread on mobile genetic elements and in bacterial chromosomes. In type I TA, synthesis of the toxin protein is prevented by the transcription of an antitoxin RNA. The first type I TA were recently identified in the human enteropathogen Clostridioides difficile. Here we report the characterization of five additional type I TA within phiCD630-1 (CD0977.1-RCd11, CD0904.1-RCd13 and CD0956.3-RCd14) and phiCD630-2 (CD2889-RCd12 and CD2907.2-RCd15) prophages of C. difficile strain 630. Toxin genes encode 34 to 47 amino acid peptides and their ectopic expression in C. difficile induces growth arrest that is neutralized by antitoxin RNA co-expression. We show that type I TA located within the phiCD630-1 prophage contribute to its stability and heritability. We have made use of a type I TA toxin gene to generate an efficient mutagenesis tool for this bacterium that allowed investigation of the role of these widespread TA in prophage maintenance.
Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway
Authors: Rico Patricia , Rodrigo-Navarro Aleixandre , Sánchez Pérez Laura , Salmeron-Sanchez Manuel 
Published: November 2020

AbstractThe intrinsic properties of mesenchymal stem cells (MSCs) make them ideal candidates for tissue engineering applications. Efforts have been made to control MSC behavior by using material systems to engineer synthetic extracellular matrices and/or include soluble factors in the media. This work proposes a simple approach based on ion transporter stimulation to determine stem cell fate that avoids the use of growth factors. Addition of borax alone, transported by the NaBC1-transporter, enhanced MSC adhesion and contractility, promoted osteogenesis and inhibited adipogenesis. Stimulated-NaBC1 promoted osteogenesis via the BMP canonical pathway (comprising Smad1/YAP nucleus translocation and osteopontin expression) through a mechanism that involves simultaneous NaBC1/BMPR1A and NaBC1/α5β1/αvβ3 co-localization. We describe an original function for NaBC1 transporter, besides controlling borate homeostasis, capable of stimulating growth factor receptors and fibronectin-binding integrins. Our results open up new biomaterial engineering approaches for biomedical applications by a cost-effective strategy that avoids the use of soluble growth factors.
Ligand-induced conformational rearrangements regulate the switch between membrane-proximal and distal functions of Rho kinase 2
Authors: Hajdú István , Szilágyi András , Végh Barbara M. , Wacha András , Györffy Dániel , Gráczer Éva , Somogyi Márk , Gál Péter , Závodszky Péter 
Published: November 2020

AbstractRho-associated protein kinase 2 (ROCK2) is a membrane-anchored, long, flexible, multidomain, multifunctional protein. Its functions can be divided into two categories: membrane-proximal and membrane-distal. A recent study concluded that membrane-distal functions require the fully extended conformation, and this conclusion was supported by electron microscopy. The present solution small-angle X-ray scattering (SAXS) study revealed that ROCK2 population is a dynamic mixture of folded and partially extended conformers. Binding of RhoA to the coiled-coil domain shifts the equilibrium towards the partially extended state. Enzyme activity measurements suggest that the binding of natural protein substrates to the kinase domain breaks up the interaction between the N-terminal kinase and C-terminal regulatory domains, but smaller substrate analogues do not. The present study reveals the dynamic behaviour of this long, dimeric molecule in solution, and our structural model provides a mechanistic explanation for a set of membrane-proximal functions while allowing for the existence of an extended conformation in the case of membrane-distal functions.
Entropic thermodynamics of nonlinear photonic chain networks
Authors: Wu Fan O. , Jung Pawel S. , Parto Midya , Khajavikhan Mercedeh , Christodoulides Demetrios N. 
Published: November 2020

AbstractThe convoluted nonlinear behaviors of heavily multimode photonic structures have been recently the focus of considerable attention. The sheer complexity associated with such multimode systems, allows them to display a host of phenomena that are otherwise impossible in few-mode settings. At the same time, however, it introduces a set of fundamental challenges in terms of comprehending and harnessing their response. Here, we develop an optical thermodynamic approach capable of describing the thermalization dynamics in large scale nonlinear photonic tight-binding networks. For this specific system, an optical Sackur-Tetrode equation is obtained that explicitly provides the optical temperature and chemical potential of the photon gas. Processes like isentropic expansion/compression, Joule expansion, as well as aspects associated with beam cleaning/cooling and thermal conduction effects in such chain networks are discussed. Our results can be used to describe in an effortless manner the exceedingly complex dynamics of highly multimoded nonlinear bosonic systems.
Enhanced dielectric constant and energy density in a BaTiO3/polymer-matrix composite sponge
Authors: Jian Gang , Jiao Yong , Meng Qingzhen , Wei Zhaoyu , Zhang Jiaoxia , Yan Chao , Moon Kyoung-Sik , Wong Ching-Ping 
Published: November 2020

AbstractPolymer-matrix dielectric composites are promising for use in electrostatic energy storage devices due to the ultra-fast charge–discharge speed and the long service life. Here we report a strategy for designing BaTiO3 sponge polymer composites for energy storage. BaTiO3 sponges with tunable porosities are prepared from polymethyl methacrylate micro-sphere arrays. Liquid epoxy completely fills the pores in a BaTiO3 sponge during vacuum de-foaming, forming a solid composite. The resulting composites possess a maximum dielectric constant of εr~332 and εr/εm~85, compared to εr~38 in a sample filled with BaTiO3 NPs, at 1 kHz. The composites also possess, at 100 kV cm−1, a high discharge energy density of Ud~105 × 10−3 J cm−3 and Ud/Um~51, and electric displacement of 3.2 μC cm−2, compared with those utilizing traditional strategies at low electric fields. Finite element simulation reveals the enhanced energy density is due to a high local electric displacement in composites.
Laser-metal interaction dynamics during additive manufacturing resolved by detection of thermally-induced electron emission
Authors: DePond Philip J. , Fuller John C. , Khairallah Saad A. , Angus Justin R. , Guss Gabe , Matthews Manyalibo J. , Martin Aiden A. 
Published: November 2020

AbstractIn situ monitoring is required to improve the understanding and increase the reliability of additive manufacturing methods such as laser powder bed fusion (LPBF). Current diagnostic methods for LPBF capture optical images, X-ray radiographs, or measure the emission of thermal or acoustic signals from the component. Herein, a methodology based on the thermal emission of electrons - thermionic emission - from the metal surface during LPBF is proposed which can resolve laser-material interaction dynamics. The high sensitivity of thermionic emission to surface temperature and surface morphology is revealed to enable precise determination of the transition between conduction and keyhole mode melting regimes. Increases in thermionic emission are correlated to laser scanning conditions that give rise to pore formation and regions where surface defects are pronounced. The information presented here is a critical step in furthering our understanding and validation of laser-based metal additive manufacturing.
β-glucan from Lentinus edodes inhibits breast cancer progression via the Nur77/HIF-1α axis
Authors: Zhang Xiuru , Li Tingting , Liu Shuwen , Xu Yiming , Meng Minjun , Li Xiumin , Lin Zhichao , Wu Qici , Xue Yu , Pan Yutian , Alitongbieke Gulimiran 
Published: November 2020

Abstract
Background: β-glucan from Lentinus edodes (LNT) is a plant-derived medicinal fungus possessing significant bioactivities on anti-tumor. Both hypoxia-induced factor-1α (HIF)-1α and Nur77 have been shown to be involved in the development of breast cancer. However, there is yet no proof of Nur77/HIF-1α involvement in the process of LNT-mediated tumor-inhibition effect.
Methods: Immunohistochemistry, immunofluorescence and Hematoxylin–Eosin staining were used to investigate tumor growth and metastasis in MMTV-PyMT transgenic mice. Proliferation and metastasis-associated molecules were determined by Western blotting and reverse transcription-quantitative PCR. Hypoxic cellular model was established under the exposure of CoCl2. Small interference RNA was transfected using Lipofectamine reagent. The ubiquitin proteasome pathway was blunted by adding the proteasome inhibitor MG132.
Results: LNT inhibited the growth of breast tumors and the development of lung metastases from breast cancer, accompanied by a decreased expression of HIF-1α in the tumor tissues. In in vitro experiments, hypoxia induced the expression of HIF-1α and Nur77 in breast cancer cells, while LNT addition down-regulated HIF-1α expression in an oxygen-free environment, and this process was in a manner of Nur77 dependent. Mechanistically, LNT evoked the down-regulation of HIF-1α involved the Nur77-mediated ubiquitin proteasome pathway. A strong positive correlation between Nur77 and HIF-1α expression in human breast cancer specimens was also confirmed.
Conclusion: Therefore, LNT appears to inhibit the progression of breast cancer partly through the Nur77/HIF-1α signaling axis. The findings of the present study may provide a theoretical basis for targeting HIFs in the treatment of breast cancer.
Collagen type I promotes osteogenic differentiation of amniotic membrane-derived mesenchymal stromal cells in basal and induction media
Authors: Akhir Haselamirrah Mohd , Teoh Peik Lin 
Published: November 2020

Abstract
Collagen has been widely shown to promote osteogenesis of bone marrow mesenchymal stromal cells (BM-MSCs). Due to the invasive procedure of obtaining BM-MSCs, MSCs from other tissues have emerged as a promising alternative for regenerative therapy. MSCs originated from different sources, exhibiting different differentiation potentials. Therefore, the applicability of collagen type I (COL), combining with amniotic membrane (AM)-MSCs was examined through proliferation and differentiation assays together with the expression of surface markers and genes associated with stemness and differentiation under basal or induction conditions. No increase in cell growth was observed because AM-MSCs might be directed toward spontaneous osteogenesis. This was evidenced by the calcium deposition and elevated expression of osteogenic genes when AM-MSCs were cultured in collagen plate with basal media. Under the osteogenic condition, reciprocal expression of OCN and CEBPA suggested a shift toward adipogenesis. Surprisingly, adipogenic genes were not elevated upon adipogenic induction, although oil droplets deposition was observed. In conclusion, our findings demonstrated that collagen causes spontaneous osteogenesis in AM-MSCs. However, the presence of exogenous inductors could shift the direction of adipo-osteogenic gene regulatory network modulated by collagen.
Red algae natural products for prevention of lipopolysaccharides (LPS)-induced liver and kidney inflammation and injuries
Authors: Nabil-Adam Asmaa , Shreadah Mohamed A. 
Published: November 2020

Abstract
Background: The liver and kidney inflammation due to bacterial infection is one of the most common pathological problems leading to tissue damage or disease. In many liver and kidney disorders, which represent serious global health burden with a high economic cost, oxidative stress-related inflammation and apoptosis are important pathogenic components, finally resulting in acute liver and/or kidney failure. Erythropoietin and its analogues are well known to influence the interaction between apoptosis and inflammation in liver and kidney. Objective: The aim of the present study is to investigate and clarify the effect of Galaxaura oblongata (G. oblongata) red algae on lipopolysaccharides (LPS)-induced acute liver and kidney injury of mice with endotoxemia and associated molecular mechanism from inflammation, apoptosis and oxidative stress levels. Results: The current study cleared out that treatment of rats with the G. oblongata extract prior to LPS injection significantly lowered serum cytokines, including NF-κB, MPO and LPO, and improved liver apoptosis through suppressing protein tyrosine kinase signaling pathway, and that may be due to antibacterial activity as well antioxidant capacity of G. oblongata extract. Conclusion: The present study was cleared out the possibility of administration of G. oblongata red algae as a multi products source for biotechnological, medical, nutraceutical and pharmaceutical applications due to highly antioxidant and anti-inflammatory capacities even although more investigations are required for separating, purifying and characterizing these bioactive compounds.
Screening and validation of differentially expressed microRNAs and target genes in hypertensive mice induced by cytomegalovirus infection
Authors: Shi YunZhong , Xi DongMei , Zhang XiaoNi , Huang Zhen , Tang Na , Liu YongMin , Wang LaMei , Tang Yan , Zhong Hua , He Fang 
Published: November 2020

Abstract
Introduction: Multiple studies have suggested an association between cytomegalovirus (CMV) infection and essential hypertension (EH). MicroRNAs (miRNAs) play a critical role in the development of EH by regulating the expression of specific target genes. However, little is known about the role of miRNAs in CMV-induced EH. In the present study, we compared the miRNA expression profiles of samples from normal and murine cytomegalovirus (MCMV)-infected C57BL/6 mice using high-throughput sequencing analysis. Methods: We collected the thoracic aorta, heart tissues, and peripheral blood from 20 normal mice and 20 MCMV-infected mice. We identified differentially expressed miRNAs in the peripheral blood samples and predicted their target genes using bioinformatics tools. We then experimentally validated them using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and the target genes with double luciferase reporter gene assay. Results: We found 118 differentially expressed miRNAs, among which 9 miRNAs were identified as potential MCMV infection-induced hypertension regulators. We then validated the expression of two candidate miRNAs, mmu-miR-1929-3p and mcmv-miR-m01-4-5p, using qRT-PCR. Furthermore, the dual-luciferase reporter gene assay revealed that the 3′-untranslated region (UTR) of endothelin A receptor (Ednra) messenger RNA (mRNA) contained a binding site for mmu-miR-1929-3p. Collectively, our data suggest that MCMV infection can raise the blood pressure and reduce mmu-miR-1929-3p expression in C57BL/6 mice. Moreover, we found that mmu-miR-1929-3p targets the 3′-UTR of the Ednra mRNA. Conclusion: This novel regulatory axis could aid the development of new approaches for the clinical prevention and control of EH.