bluuebunny/crossref_metadata_2025_split huggingface dataset:
Including the Temporal Dimension in the SECS Technique
Authors: Marsal S. , Torta J. M. , Pavón‐Carrasco F. J. , Blake S. P. , Piersanti M. 
Published: June 2020

AbstractThe equivalent source method of Spherical Elementary Current Systems (SECS) has contributed valuable results for spatial magnetic interpolation purposes where no observations are available, as well as for modeling equivalent currents both in the ionosphere and in the subsurface, thus providing a separation between external and internal sources. It has been successfully applied to numerous Space Weather (SW) events, whereas some advantages have been reported over other techniques such as Fourier or Spherical (Cap) Harmonic Analysis. Although different modalities of SECS exist (either 1‐D, 2‐D, or 3‐D) depending on the number of space dimensions involved, the method provides a sequence of instantaneous pictures of the source current. We present an extension of SECS consisting in the introduction of a temporal dependence in the formulation based on a cubic B‐splines expansion. The technique thus adds one dimension, becoming 4‐D in general (e.g., 3‐D + t), and its application is envisaged for, though not restricted to, the analysis of past events including heterogeneous geomagnetic data sets, such as those containing gaps, different sampling rates or diverse data sources. A synthetic model based on the SW Modeling Framework is used to show the efficacy of the extended scheme. We apply this method to characterize the current systems of past and significant SW events producing geomagnetically induced currents, which we exemplify with an outstanding geomagnetic sudden commencement occurred on 24 March 1991.
Streamflow Recession Analysis Using Water Height
Authors: Jachens E. R. , Roques C. , Rupp D. E. , Selker J. S. 
Published: June 2020

AbstractRecession analysis is widely used for characterizing aquifer and basin properties based on the falling limb of the hydrograph. However, recession analysis using streamflow discharge requires a relationship (the rating curve) between simultaneous measurements of water height, h, and discharge, Q, across a wide range of flows, which is expensive to obtain, and changes in time. We leverage the relationship between h and Q (typical power law) to perform recession analysis using h directly, thus permitting identification of transient flow regimes, where only h is available. Recession analysis evaluates the rate of change in discharge, −dQ/dt, as a function of discharge, Q, in a bilogarithmic plot where the slope, b, contains information about aquifer characteristics. While values of b are not conserved when replacing Q with h, we find that the variability in values of b within and between events is captured in recession analysis for both Q and h. For example, when considering individual recessions from a large number of watersheds, a change from a smaller b at high discharge/stage to a larger b at lower discharge/stage occurs in most watersheds and suggests a transition to a more long‐lasting, and less drought‐sensitive, baseflow regime. With the advent of low‐cost reliable pressure loggers, as well as satellites that provide global reporting of river stage, recession analysis using water height expands the number of river systems where recession analysis can be conducted and provides the potential for insights into the variability of watershed drainage characteristics without the need for a discharge record.
Measuring Aquifer Specific Yields With Absolute Gravimetry: Result in the Choushui River Alluvial Fan and Mingchu Basin, Central Taiwan
Authors: Chen Kuan‐Hung , Hwang Cheinway , Chang Liang‐Cheng , Tsai Jui‐Pin , Yeh Tian‐Chyi Jim , Cheng Ching‐Chung , Ke Chien‐Chung , Feng Wei 
Published: June 2020

AbstractAccurate and densely covered specific yields (Sy) are essential for estimating the storage capacity of a groundwater reservoir. A cross‐well pumping test can determine Sy, but its high cost often makes it unsuitable for sampling high‐resolution Sy. The gravity‐based method (GBM) based on gravity changes near existing groundwater wells may outperform cross‐well pumping tests in estimating Sy. We established 10 gravity sites close to groundwater wells in the aquifer‐rich Choushui River Alluvial Fan and Mingchu Basin in central Taiwan and measured gravity changes with two FG5 gravimeters from 2012 to 2017. Thirty‐nine Sy values with formal errors are determined by natural rises and falls in gravity and groundwater level. The representative Sy values (0.04 to 0.29) from GBM are in general consistent with those from cross‐well pumping tests (0.03 to 0.24). Repeated groundwater level changes over similar depth range at different times serve as revisit tests, showing that GBM can reproduce a reliable Sy value at a given site and depth. Soil moisture and compaction data show that the effects of gravity changes originating from unsaturated zones and deep aquifer layers are minor. Using the cylinder model for aquifers with limited lateral extents, we assess the validity of the Bouguer model by quantifying gravity differences and relative Sy differences originating from the model assumption. Improvements in environmental resilience and transportability achieved by recent atomic gravimeters may increase the potential of GBM to replace or supplement cross‐well pumping tests in densifying Sy point densities for an improved groundwater resource management.
Ghost spintronic THz-emitter-array microscope
Authors: Chen Si-Chao , Feng Zheng , Li Jiang , Tan Wei , Du Liang-Hui , Cai Jianwang , Ma Yuncan , He Kang , Ding Haifeng , Zhai Zhao-Hui , Li Ze-Ren , Qiu Cheng-Wei , Zhang Xi-Cheng , Zhu Li-Guo 
Published: June 2020

AbstractTerahertz (THz) waves show great potential in nondestructive testing, biodetection and cancer imaging. Despite recent progress in THz wave near-field probes/apertures enabling raster scanning of an object’s surface, an efficient, nonscanning, noninvasive, deep subdiffraction imaging technique remains challenging. Here, we demonstrate THz near-field microscopy using a reconfigurable spintronic THz emitter array (STEA) based on the computational ghost imaging principle. By illuminating an object with the reconfigurable STEA followed by computing the correlation, we can reconstruct an image of the object with deep subdiffraction resolution. By applying an external magnetic field, in-line polarization rotation of the THz wave is realized, making the fused image contrast polarization-free. Time-of-flight (TOF) measurements of coherent THz pulses further enable objects at different distances or depths to be resolved. The demonstrated ghost spintronic THz-emitter-array microscope (GHOSTEAM) is a radically novel imaging tool for THz near-field imaging, opening paradigm-shifting opportunities for nonintrusive label-free bioimaging in a broadband frequency range from 0.1 to 30 THz (namely, 3.3–1000 cm−1).
Diagnosing metabolic syndrome in a multi-ethnic country: is an ethnic-specific cut-off point of waist circumference needed?
Authors: Tahapary Dicky L. , Harbuwono Dante S. , Yunir Em , Soewondo Pradana 
Published: June 2020

AbstractThe definition of Metabolic Syndrome (MS) required an ethnic-specific cut-off point for waist circumference (WC). We aim to assess the optimal ethnic-specific WC cut-off point for MS in Indonesia, a multi-ethnic country. Three population-based studies in Indonesia were included for analysis [Flores (n = 1227, Floresian), Depok (n = 904, Sundanese), and Jakarta (n = 1574, Javanese)]. All subjects were 25–65 years old. The receiver operator characteristic curve analysis and Youden index method was used to determine the optimal cut-offs of WC to predict two or more risk factors of MS. In Flores, the cut-offs were 80 cm (Sensitivity and Specificity, AUC, 84% and 73%, 0.86) and 77 cm (86% and 68%, 0.85), for men and women, respectively. While in Depok, the values were 87 cm (87% and 67%, 0.85) and 79 cm (94% and 54%, 0.79), for men and women, respectively. While in Jakarta, the values were 83 cm (92% and 60%, 0.85) and 81 cm (84% and 60%, 0.77), for men and women, respectively. The optimal WC cut-off values for MS were different in those three ethnicities, and in general were lower than the currently used cut-off points for Asian population.
Melatonin-induced ApoE expression in mouse astrocytes protects endothelial cells from OGD-R induced injuries
Authors: Xiang Jun , Zhu Wen , Yang Feng , Yu Zhong-Hai , Cai Min , Li Xiang-Ting , Zhang Jing-Si , Zhang Wen , Cai Ding-Fang 
Published: June 2020

AbstractStroke is a leading reason of death and long-term disability, and most studies mainly focus on efforts to protect neurons. However, failed clinical trials suggest that therapies against single target in neurons may not be sufficient and the involvement of endothelial cells and glial cells have been underestimated. Astrocytes are the major source of ApoE in the brain and endothelial cells express high level of ApoE receptors. Thus, ApoE may mediate the interaction between astrocytes and endothelial cells. To address whether and how ApoE-mediated astrocytes–endothelial cells interaction contributes to the pathogenesis of stroke, we used oxygen and glucose deprivation-reoxygenation (OGD-R) as a stroke model and investigated the effects of OGD-R on astrocytes-endothelial cell co-cultures in the current study. We find that OGD-R leads to various damages to endothelial cells, including compromised cell viability, increased ROS level, enhanced caspase activity, and higher apoptotic rate. Meanwhile, mouse astrocytes could secrete ApoE to activate PI3K/eNOS signaling in endothelial cells to prevent OGD-R induced injuries. In addition, OGD-R induces down-regulation of ApoE in astrocyte–endothelial cell co-cultures while melatonin restores astrocytic ApoE expression via pCREB pathway and protects endothelial cell in OGD-R treated co-cultures. Our study provides evidence that astrocytes could protect endothelial cells via ApoE in OGD-R condition and Melatonin could induce ApoE expression to protect endothelial cells.
Investigating resting brain perfusion abnormalities and disease target-engagement by intranasal oxytocin in women with bulimia nervosa and binge-eating disorder and healthy controls
Authors: Martins Daniel , Leslie Monica , Rodan Sarah , Zelaya Fernando , Treasure Janet , Paloyelis Yannis 
Published: June 2020

AbstractAdvances in the treatment of bulimia nervosa and binge-eating disorder (BN/BED) have been marred by our limited understanding of the underpinning neurobiology. Here we measured regional cerebral blood flow (rCBF) to map resting perfusion abnormalities in women with BN/BED compared with healthy controls and investigate whether intranasal oxytocin (OT), proposed as a potential treatment, can restore perfusion in disorder-related brain circuits. Twenty-four women with BN/BED and 23 healthy women participated in a randomized, double-blind, crossover, placebo-controlled study. We used arterial spin labelling MRI to measure rCBF and the effects of an acute dose of intranasal OT (40 IU) or placebo over 18–26 min post dosing, as we have previously shown robust OT-induced changes in resting rCBF in men in a similar time-window (15–36 min post dosing). We tested for effects of treatment, diagnosis and their interaction on extracted rCBF values in anatomical regions-of-interest previously implicated in BN/BED by other neuroimaging modalities, and conducted exploratory whole-brain analyses to investigate previously unidentified brain regions. We demonstrated that women with BN/BED presented increased resting rCBF in the medial prefrontal and orbitofrontal cortices, anterior cingulate gyrus, posterior insula and middle/inferior temporal gyri bilaterally. Hyperperfusion in these areas specifically correlated with eating symptoms severity in patients. Our data did not support a normalizing effect of intranasal OT on perfusion abnormalities in these patients, at least for the specific dose (40 IU) and post-dosing interval (18–26 min) examined. Our findings enhance our understanding of resting brain abnormalities in BN/BED and identify resting rCBF as a non-invasive potential biomarker for disease-related changes and treatment monitoring. They also highlight the need for a comprehensive investigation of intranasal OT pharmacodynamics in women before we can fully ascertain its therapeutic value in disorders affecting predominantly this gender, such as BN/BED.
COVID-19 infection: the China and Italy perspectives
Authors: Chen Jun , Lu Hongzhou , Melino Gerry , Boccia Stefania , Piacentini Mauro , Ricciardi Walter , Wang Ying , Shi Yufang , Zhu Tongyu 
Published: June 2020

AbstractThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the COVID-19 pandemic. Since its first report in December 2019, despite great efforts made in almost every country worldwide, this disease continues to spread globally, especially in most parts of Europe, Iran, and the United States. Here, we update the recent understanding in clinical characteristics, diagnosis strategies, as well as clinical management of COVID-19 in China as compared to Italy, with the purpose to integrate the China experience with the global efforts to outline references for prevention, basic research, treatment as well as final control of the disease. Being the first two countries we feel appropriate to evaluate the evolution of the disease as well as the early result of the treatment, in order to offer a different baseline to other countries. It is also interesting to compare two countries, with a very significant difference in population, where the morbidity and mortality has been so different, and unrelated to the size of the country.
Intraperitoneal injection of IFN-γ restores microglial autophagy, promotes amyloid-β clearance and improves cognition in APP/PS1 mice
Authors: He Zitian , Yang Yunjie , Xing Zhiwei , Zuo Zejie , Wang Rui , Gu Huaiyu , Qi Fangfang , Yao Zhibin 
Published: June 2020

AbstractAutophagy is a major self-degradative process that maintains cellular homeostasis and function in mammalian cells. Autophagic dysfunction occurs in the early pathogenesis of Alzheimer’s disease (AD) and directly regulates amyloid-β (Aβ) metabolism. Although it has been proven that the cytokine IFN-γ enhances autophagy in macrophage cell lines, whether the signaling cascade is implicated in Aβ degradation in AD mouse models remains to be elucidated. Here, we found that 9 days of the intraperitoneal administration of IFN-γ significantly increased the LC3II/I ratio and decreased the level of p62 in APP/PS1 mice, an AD mouse model. In vitro, IFN-γ protected BV2 cells from Aβ toxicity by upregulating the expressions of Atg7 and Atg5 and the LC3II/I ratio, whereas these protective effects were ablated by interference with Atg5 expression. Moreover, IFN-γ enhanced autophagic flux, probably through suppressing the AKT/mTOR pathway both in vivo and in vitro. Importantly, using intravital two-photon microscopy and fluorescence staining, we found that microglia interacted with exogenous IFN-γ and Aβ, and surrounded Aβ in APP/PS1;CX3CR1-GFP+/− mice. In addition, IFN-γ treatment decreased the Aβ plaque load in the cortex and hippocampus and rescued cognitive deficits in APP/PS1 mice. Our data suggest a possible mechanism by which the peripheral injection of IFN-γ restores microglial autophagy to induce the phagocytosis of cerebral Aβ, which represents a potential therapeutic approach for the use of exogenous IFN-γ in AD.
Aberrant activation of neuronal cell cycle caused by dysregulation of ubiquitin ligase Itch results in neurodegeneration
Authors: Chauhan Monika , Modi Prashant Kumar , Sharma Pushkar 
Published: June 2020

AbstractIt is critical for the neuronal cell cycle to remain suppressed in terminally differentiated neurons as its activation results in aberrant cell cycle re-entry that causes neuronal apoptosis (CRNA), which has been observed in several neurodegenerative disorders like Alzheimer’s disease (AD). In the present study, we report that E3 ubiquitin ligase Itch is a major regulator of CRNA and elucidated the mechanism via which it is regulated in this process. Neurotoxic amyloid peptide Aβ42-treated neurons or neurons from an AD transgenic mouse model (TgAD) exhibited aberrant activation of the JNK pathway which resulted in the hyperphosphorylation of Itch. The phosphorylation of Itch primes it for autoubiquitination, which is necessary for its activation. These post-translational modifications of Itch facilitate its interaction with TAp73 resulting in its degradation. These series of events are critical for Itch-mediated CRNA and its phosphorylation and autoubiquitination site mutants reversed this process and were neuroprotective. These studies unravel a novel pathway via which neurodegeneration in AD and possibly other related disorders may be regulated by aberrant regulation of the neuronal cell cycle.
Novel XIAP mutation causing enhanced spontaneous apoptosis and disturbed NOD2 signalling in a patient with atypical adult-onset Crohn’s disease
Authors: Parackova Zuzana , Milota Tomas , Vrabcova Petra , Smetanova Jitka , Svaton Michael , Freiberger Tomas , Kanderova Veronika , Sediva Anna 
Published: June 2020

AbstractX-linked inhibitor of apoptosis (XIAP) is the most potent human inhibitor of apoptosis, and is also involved in NOD2-dependent NFκB and MAPK signalling cascade activation. The absence or defective function of XIAP leads to the development of a rare and severe primary immunodeficiency known as X-linked lymphoproliferative syndrome type 2 (XLP-2), which is characterized by a triad of clinical manifestations, including a high incidence of haemophagocytic lymphohistiocytosis (HLH), lymphoproliferation and inflammatory bowel disease (IBD), usually with very early onset. Here, we present a novel XIAP mutation identified in a patient with atypical adult-onset IBD complicated by relapsing HLH, splenomegaly and sarcoid-like disease. The c.266delA mutation in the XIAP gene creates a premature stop codon, and causes a severe reduction in XIAP protein expression. The mutation is also associated with impaired spontaneous and staurosporine- and PMA-induced apoptosis accompanied by significantly increased expression of pro-apoptotic genes. We also confirmed the negative impact of this particular XIAP mutation on NOD2-dependent NFκB and MAPK activation, while NOD2-independent activation was found to be unaffected. Moreover, we assume that the mutation has an impact on the overproduction of IL-12 and IFNγ, the shift towards the Th1 immune response and increased numbers of central memory and effector memory CD4+ and CD8+ T cells. All these changes contribute to immune dysregulation and the clinical manifestation of XLP-2.
Kinase inhibition of G2019S-LRRK2 enhances autolysosome formation and function to reduce endogenous alpha-synuclein intracellular inclusions
Authors: Obergasteiger Julia , Frapporti Giulia , Lamonaca Giulia , Pizzi Sara , Picard Anne , Lavdas Alexandros A. , Pischedda Francesca , Piccoli Giovanni , Hilfiker Sabine , Lobbestael Evy , Baekelandt Veerle , Hicks Andrew A. , Corti Corrado , Pramstaller Peter P. , Volta Mattia 
Published: June 2020

AbstractThe Parkinson’s disease (PD)-associated kinase Leucine-Rich Repeat Kinase 2 (LRRK2) is a crucial modulator of the autophagy-lysosome pathway, but unclarity exists on the precise mechanics of its role and the direction of this modulation. In particular, LRRK2 is involved in the degradation of pathological alpha-synuclein, with pathogenic mutations precipitating neuropathology in cellular and animal models of PD, and a significant proportion of LRRK2 patients presenting Lewy neuropathology. Defects in autophagic processing and lysosomal degradation of alpha-synuclein have been postulated to underlie its accumulation and onset of neuropathology. Thus, it is critical to obtain a comprehensive knowledge on LRRK2-associated pathology. Here, we investigated a G2019S-LRRK2 recombinant cell line exhibiting accumulation of endogenous, phosphorylated alpha-synuclein. We found that G2019S-LRRK2 leads to accumulation of LC3 and abnormalities in lysosome morphology and proteolytic activity in a kinase-dependent fashion, but independent from constitutively active Rab10. Notably, LRRK2 inhibition was ineffective upon upstream blockade of autophagosome-lysosome fusion events, highlighting this step as critical for alpha-synuclein clearance.
Inflammasome activation and regulation: toward a better understanding of complex mechanisms
Authors: Zheng Danping , Liwinski Timur , Elinav Eran 
Published: June 2020

AbstractInflammasomes are cytoplasmic multiprotein complexes comprising a sensor protein, inflammatory caspases, and in some but not all cases an adapter protein connecting the two. They can be activated by a repertoire of endogenous and exogenous stimuli, leading to enzymatic activation of canonical caspase-1, noncanonical caspase-11 (or the equivalent caspase-4 and caspase-5 in humans) or caspase-8, resulting in secretion of IL-1β and IL-18, as well as apoptotic and pyroptotic cell death. Appropriate inflammasome activation is vital for the host to cope with foreign pathogens or tissue damage, while aberrant inflammasome activation can cause uncontrolled tissue responses that may contribute to various diseases, including autoinflammatory disorders, cardiometabolic diseases, cancer and neurodegenerative diseases. Therefore, it is imperative to maintain a fine balance between inflammasome activation and inhibition, which requires a fine-tuned regulation of inflammasome assembly and effector function. Recently, a growing body of studies have been focusing on delineating the structural and molecular mechanisms underlying the regulation of inflammasome signaling. In the present review, we summarize the most recent advances and remaining challenges in understanding the ordered inflammasome assembly and activation upon sensing of diverse stimuli, as well as the tight regulations of these processes. Furthermore, we review recent progress and challenges in translating inflammasome research into therapeutic tools, aimed at modifying inflammasome-regulated human diseases.
Unusual context of CENPJ variants and primary microcephaly: compound heterozygosity and nonconsanguinity in an Argentinian patient
Authors: Cueto-González Anna M. , Fernández-Cancio Mónica , Fernández-Alvarez Paula , García-Arumí Elena , Tizzano Eduardo F. 
Published: June 2020

AbstractPrimary microcephaly (MCPH) is a genetically heterogeneous disorder showing an autosomal recessive mode of inheritance. Patients with MCPH present head circumference values two or three standard deviations (SDs) significantly below the mean for age- and sex-matched populations. MCPH is associated with a nonprogressive mild to severe intellectual disability, with normal brain structure in most patients, or with a small brain and gyri without visceral malformations. We present the case of an adult patient born from Argentinian nonconsanguineous healthy parents. He had a head circumference >5 SD below the mean, cerebral neuroimaging showing hypoplasia of the corpus callosum, bilateral migration disorder with heterotopia of the sylvian fissure and colpocephaly. The patient was compound heterozygous for pathogenic variants in the CENPJ gene (c.289dupA inherited from his mother and c.1132 C > T inherited from his father). Our patient represents an uncommon situation for the usual known context of CENPJ and MCPH, including family origin (Argentinian), pedigree (nonconsanguineous), and genotype (a compound heterozygous case with two variants predicting a truncated protein). Next-generation sequencing studies applied in a broader spectrum of clinical presentations of MCPH syndromes may discover additional similar patients and families.