bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adolescent polycystic ovary syndrome according to the international evidence-based guideline
Authors: Peña Alexia S. , Witchel Selma F. , Hoeger Kathleen M. , Oberfield Sharon E. , Vogiatzi Maria G. , Misso Marie , Garad Rhonda , Dabadghao Preeti , Teede Helena 
Published: March 2020

Abstract
Background
Diagnosing polycystic ovary syndrome (PCOS) during adolescence is challenging because features of normal pubertal development overlap with adult diagnostic criteria. The international evidence-based PCOS Guideline aimed to promote accurate and timely diagnosis, to optimise consistent care, and to improve health outcomes for adolescents and women with PCOS.

Methods
International healthcare professionals, evidence synthesis teams and consumers informed the priorities, reviewed published data and synthesised the recommendations for the Guideline. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) framework was applied to appraise the evidence quality and the feasibility, acceptability, cost, implementation and strength of the recommendations.

Results
This paper focuses on the specific adolescent PCOS Guideline recommendations. Specific criteria to improve diagnostic accuracy and avoid over diagnosis include: (1) irregular menstrual cycles defined according to years post-menarche; > 90 days for any one cycle (> 1 year post-menarche), cycles< 21 or > 45 days (> 1 to < 3 years post-menarche); cycles < 21 or > 35 days (> 3 years post-menarche) and primary amenorrhea by age 15 or > 3 years post-thelarche. Irregular menstrual cycles (< 1 year post-menarche) represent normal pubertal transition. (2) Hyperandrogenism defined as hirsutism, severe acne and/or biochemical hyperandrogenaemia confirmed using validated high-quality assays. (3) Pelvic ultrasound not recommended for diagnosis of PCOS within 8 years post menarche. (4) Anti-Müllerian hormone levels not recommended for PCOS diagnosis; and (5) exclusion of other disorders that mimic PCOS. For adolescents who have features of PCOS but do not meet diagnostic criteria an ‘at risk’ label can be considered with appropriate symptomatic treatment and regular re-evaluations. Menstrual cycle re-evaluation can occur over 3 years post menarche and where only menstrual irregularity or hyperandrogenism are present initially, evaluation with ultrasound can occur after 8 years post menarche. Screening for anxiety and depression is required and assessment of eating disorders warrants consideration. Available data endorse the benefits of healthy lifestyle interventions to prevent excess weight gain and should be recommended. For symptom management, the combined oral contraceptive pill and/or metformin may be beneficial.

Conclusions
Extensive international engagement accompanied by rigorous processes honed both diagnostic criteria and treatment recommendations for PCOS during adolescence.
Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI
Authors: Morse Leslie R. , Nguyen Nguyen , Xu Yan , Jha Prakash , Battaglino Ricardo A. 
Published: March 2020

Abstract
Background
Peroxiredoxin activated in M-CSF stimulated monocytes (PAMM) is a novel protein produced by adipocytes with putative redox regulatory and anti-inflammatory properties. Because acute spinal cord injury (SCI) is associated with oxidative stress and neuroinflammation and because PAMM can be detected in systemic circulation, we hypothesized that acute neuro-trauma might induce changes in circulating PAMM expression. Specifically, we hypothesized that PAMM levels might vary based on the presence or absence of acute, traumatic SCI. We therefore investigated circulating PAMM levels in adults with and without acute traumatic SCI.

Methods
We studied 105 men and women (54 with SCI and 51 without SCI). Participants with SCI were admitted for acute rehabilitation within 1 month after injury. Serum samples were obtained during hospitalization and stored at − 80 °C until batch analysis. Total PAMM was quantified by ELISA assay (MyBiosource, Cat. No: MBS9327247) with a detection limit of 0.25 ng/ml. Separate multivariate models including age, BMI, and injury severity were assessed to determine significant clinical predictors of change in PAMM levels.

Results
When adjusting for BMI, age, and gender, mean change in PAMM levels were greatest in participants with motor complete SCI compared to able-bodied (1.65 ng/ml versus 0.94 ng/ml, p = 0.003). This model explained 26% of the variation in change in circulating PAMM levels.

Conclusions
Our results suggest that PAMM may be a novel biomarker of neurological injury or of native anti-inflammatory responses to neurological injury. More work is needed to establish the role of PAMM and other adipocyte-derived factors in the acute response to neurotrauma.
Role of dietary fatty acids in microglial polarization in Alzheimer’s disease
Authors: Desale Smita Eknath , Chinnathambi Subashchandrabose 
Published: March 2020

AbstractMicroglial polarization is an utmost important phenomenon in Alzheimer’s disease that influences the brain environment. Polarization depends upon the types of responses that cells undergo, and it is characterized by receptors present on the cell surface and the secreted cytokines to the most. The expression of receptors on the surface is majorly influenced by internal and external factors such as dietary lipids. Types of fatty acids consumed through diet influence the brain environment and glial cell phenotype and types of receptors on microglia. Reports suggest that dietary habits influence microglial polarization and the switching of microglial phenotype is very important in neurodegenerative diseases. Omega-3 fatty acids have more influence on the brain, and they are found to regulate the inflammatory stage of microglia by fine-tuning the number of receptors expressed on microglia cells. In Alzheimer’s disease, one of the pathological proteins involved is Tau protein, and microtubule-associated protein upon abnormal phosphorylation detaches from the microtubule and forms insoluble aggregates. Aggregated proteins have a tendency to propagate within the neurons and also become one of the causes of neuroinflammation. We hypothesize that tuning microglia towards anti-inflammatory phenotype would reduce the propagation of Tau in Alzheimer’s disease.
Psychological functioning in children suspected for mitochondrial disease: the need for care
Authors: van de Loo Kim F. E. , Custers José A. E. , Koene Saskia , Klein Inge-Lot , Janssen Mirian C. H. , Smeitink Jan A. M. , Verhaak Christianne M. 
Published: March 2020

Abstract
Background
Mitochondrial diseases (MD) are generally serious and progressive, inherited metabolic diseases. There is a high comorbidity of anxiety and depression and limitations in daily functioning. The complexity and duration of the diagnostic process and lack of knowledge about prognosis leads to uncertainty. In this study, we investigated the psychological well-being of children who are suspected for MD and their parents.

Methods
In total 122 children suspected for MD and their parents, received questionnaires as part of standard clinical investigation.

Results
Parent proxy report revealed a lower quality of life (QoL) compared to norms and even more physical problems compared to chronically ill patients. They also reported more behavioral problems in general and more internalizing problems compared to the norms. Most frequent reported somatic complaints were tiredness and pain. Parents did not report enhanced levels of stress regarding parenting and experienced sufficient social support. At the end of the diagnostic process, 5.7% of the children received the genetically confirmed diagnosis of MD, 26% showed non-conclusive abnormalities in the muscle biopsy, 54% did not receive any diagnosis, and the remaining received other diagnoses. Strikingly, children without a diagnosis showed equally QoL and behavioral problems as children with a diagnosis, and even more internalizing problems.

Conclusions
This study highlights the psychological concerns of children with a suspicion of MD. It is important to realize that as well as children with a confirmed diagnosis, children without a diagnosis are vulnerable since explanation for their complaints is still lacking.
Therapeutic Potential of the Gut Microbiota in the Management of Sepsis
Authors: Bassetti Matteo , Bandera Alessandra , Gori Andrea 
Published: March 2020

AbstractThis article is one of ten reviews selected from the Annual Update in Intensive Care and Emergency Medicine 2020. Other selected articles can be found online at https://www.biomedcentral.com/collections/annualupdate2020. Further information about the Annual Update in Intensive Care and Emergency Medicine is available from http://www.springer.com/series/8901.
A Clinician’s Guide to Management of Intra-abdominal Hypertension and Abdominal Compartment Syndrome in Critically Ill Patients
Authors: De Laet Inneke E. , Malbrain Manu L. N. G. , De Waele Jan J. 
Published: March 2020

AbstractThis article is one of ten reviews selected from the Annual Update in Intensive Care and Emergency Medicine 2020. Other selected articles can be found online at https://www.biomedcentral.com/collections/annualupdate2020. Further information about the Annual Update in Intensive Care and Emergency Medicine is available from http://www.springer.com/series/8901.
Respiratory Muscle Rehabilitation in Patients with Prolonged Mechanical Ventilation: A Targeted Approach
Authors: Bissett Bernie , Gosselink Rik , van Haren Frank M. P. 
Published: March 2020

AbstractThis article is one of ten reviews selected from the Annual Update in Intensive Care and Emergency Medicine 2020. Other selected articles can be found online at https://www.biomedcentral.com/collections/annualupdate2020. Further information about the Annual Update in Intensive Care and Emergency Medicine is available from http://www.springer.com/series/8901.
Burnout and Joy in the Profession of Critical Care Medicine
Authors: Kerlin Meeta Prasad , McPeake Joanne , Mikkelsen Mark E. 
Published: March 2020

AbstractThis article is one of ten reviews selected from the Annual Update in Intensive Care and Emergency Medicine 2020. Other selected articles can be found online at https://www.biomedcentral.com/collections/annualupdate2020. Further information about the Annual Update in Intensive Care and Emergency Medicine is available from http://www.springer.com/series/8901.
Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction
Authors: Fastrès Aline , Canonne Morgane A. , Taminiau Bernard , Billen Frederic , Garigliany Mutien-Marie , Daube Georges , Clercx Cécile 
Published: March 2020

AbstractInfection with Bordetella bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bb in all diseased dogs, about half being co-infected with Mc. In diseased compared with healthy dogs, the β-diversity changed (P = 0.0024); bacterial richness and α-diversity were lower (P = 0.012 and 0.0061), and bacterial load higher (P = 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P = 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases.
Some inequalities related to strong convergence of Riesz logarithmic means
Authors: Lukkassen D. , Persson L. E. , Tephnadze G. , Tutberidze G. 
Published: March 2020

AbstractIn this paper we derive a new strong convergence theorem of Riesz logarithmic means of the one-dimensional Vilenkin–Fourier (Walsh–Fourier) series. The corresponding inequality is pointed out and it is also proved that the inequality is in a sense sharp, at least for the case with Walsh–Fourier series.
A new error estimate on uniform norm of a parabolic variational inequality with nonlinear source terms via the subsolution concepts
Authors: Boulaaras Salah , Bencheikh Le Hocine Mohamed El Amine , Haiour Mohamed 
Published: March 2020

AbstractThis paper deals with the numerical analysis of parabolic variational inequalities with nonlinear source terms, where the existence and uniqueness of the solution is provided by using Banach’s fixed point theorem. In addition, an optimally $L^{\infty}$L∞-asymptotic behavior is proved using Euler time scheme combined with the finite element spatial approximation. The approach is based on Bensoussan–Lions algorithm for evolutionary free boundary problems using the concepts of subsolutions.
Spatio-temporal co-occurrence of hotspots of tuberculosis, poverty and air pollution in Lima, Peru
Authors: Carrasco-Escobar Gabriel , Schwalb Alvaro , Tello-Lizarraga Kelly , Vega-Guerovich Percy , Ugarte-Gil Cesar 
Published: March 2020

AbstractGrowing evidence suggests pollution and other environmental factors have a role in the development of tuberculosis (TB), however, such studies have never been conducted in Peru. Considering the association between air pollution and specific geographic areas, our objective was to determine the spatial distribution and clustering of TB incident cases in Lima and their co-occurrence with clusters of fine particulate matter (PM2.5) and poverty. We found co-occurrences of clusters of elevated concentrations of air pollutants such as PM2.5, high poverty indexes, and high TB incidence in Lima. These findings suggest an interplay of socio-economic and environmental in driving TB incidence.
Surgical management and prognostic factors of spinal metastatic tumors
Authors: Elmesallamy Wael A. A. , Taha Mahmoud M. 
Published: March 2020

Abstract
Objective
This study aims to evaluate different prognostic factors after surgical management of metastatic spinal tumors regarding clinical condition, preoperative investigations, histopathological results, and surgical data.

Methods
Seventy patients diagnosed as metastatic spinal tumors with neurological deficits and/or unstable spine operated for spinal decompression with or without instrumental fixation according to Spinal Instability Neoplastic Score (SINS) at our institute during the period from May 2014 to October 2018 with follow-up at least 9 months.

Results
Lymphoma metastases were the commonest spinal metastases of 23% with significant p value = 0.001, males and ages above 50 years old were significantly affected. High vascularity and bone invasion were significant operative findings. Significant good prognostic factors for both survival and Klekampe score improvement were paretic patients, > 15 preoperative Klekampe score, early surgery, ≤ 3 vertebral affection, extradural tumor location, gross total resection, and metastatic tumors from multiple myeloma, thyroid gland, lymphoma, and prostatic gland.

Conclusion
Early surgeries aiming neural decompression and keeping spinal stability according to Spinal Instability Neoplastic Score for patients with spinal metastases are the main hope for better survival and neurological improvement.
Total Number of Alterations in Liquid Biopsies Is an Independent Predictor of Survival in Patients With Advanced Cancers
Authors: Vu Peter , Khagi Yulian , Riviere Paul , Goodman Aaron , Kurzrock Razelle 
Published: March 2020

PURPOSE Studies have demonstrated an association between quantity of circulating tumor DNA (ctDNA) and poorer survival. We investigated the relationship between percent ctDNA (%ctDNA), total number of ctDNA alterations, and overall survival (OS) in liquid biopsies. MATERIALS AND METHODS Overall, 418 patients with blood-based next-generation sequencing (54 to 73 genes) were analyzed. Eligible patients included those who had advanced/metastatic solid tumor malignancies and never received immunotherapy treatment, which may alter the survival curve in patients with high mutational burden. RESULTS Patients with a high (≥ 5%) %ctDNA had significantly shorter OS versus those with intermediate (≥ 0.4% to < 5%) or low (< 0.4%) values (median OS, 7.0 v 14.1 v not reached [NR] months, respectively; P < .0001). Patients with a high (≥ 5) total number of alterations had significantly shorter OS versus those with intermediate (≥ 1.46 to < 5), low (< 1.46), or no alterations (median OS, 4.6 v 11.7 v 21.3 v NR months, respectively; P < .0001). The total number of alterations correlated with %ctDNA (r = 0.85; 95% CI, 0.81 to 0.87; P < .0001). However, only an intermediate to high total number of alterations (≥ 1.46) was an independent predictor of worse OS (hazard ratio, 1.96; 95% CI, 1.30 to 2.96; P = .0014; multivariate analysis). CONCLUSION We demonstrate that the total number of alterations and %ctDNA have prognostic value and correlate with one another, but only the total number of alterations was independently associated with survival outcomes. Our findings suggest that the total number of alterations in plasma may be an indicator of more aggressive tumor biology and therefore poorer survival.
A “Step–Ramp–Step” Protocol to Identify the Maximal Metabolic Steady State
Authors: IANNETTA DANILO , INGLIS ERIN CALAINE , POGLIAGHI SILVIA , MURIAS JUAN M. , KEIR DANIEL A. 
Published: March 2020

ABSTRACT
The oxygen uptake (V̇O2) at the respiratory compensation point (RCP) closely identifies with the maximal metabolic steady state. However, the power output (PO) at RCP cannot be determined from contemporary ramp-incremental exercise protocols.

Purpose
This study aimed to test the efficacy of a “step–ramp–step” (SRS) cycling protocol for estimating the PO at RCP and the validity of RCP as a maximal metabolic steady-state surrogate.

Methods
Ten heathy volunteers (5 women; age: 30 ± 7 yr; V̇O2max: 54 ± 6 mL·kg−1·min−1) performed in the following series: a moderate step transition to 100 W (MOD), ramp (30 W·min−1), and after 30 min of recovery, step transition to ~50% POpeak (HVY). Ventilatory and gas exchange data from the ramp were used to identify the V̇O2 at lactate threshold (LT) and RCP. The PO at LT was determined by the linear regression of the V̇O2 versus PO relationship after adjusting ramp data by the difference between the ramp PO at the steady-state V̇O2 from MOD and 100 W. Linear regression between the V̇O2–PO values associated with LT and HVY provided, by extrapolation, the PO at RCP. Participants then performed 30-min constant-power tests at the SRS-estimated RCP and 5% above this PO.

Results
All participants completed 30 min of constant-power exercise at the SRS-estimated RCP achieving steady-state V̇O2 of 3176 ± 595 mL·min−1 that was not different (P = 0.80) from the ramp-identified RCP (3095 ± 570 mL·min−1) and highly consistent within participants (bias = −26 mL·min−1, r = 0.97, coefficient of variation = 2.3% ± 2.8%). At 5% above the SRS-estimated RCP, four participants could not complete 30 min and all, but two exhibited non–steady-state responses in blood lactate and V̇O2.

Conclusions
In healthy individuals cycling at their preferred cadence, the SRS protocol and the RCP are capable of accurately predicting the PO associated with maximal metabolic steady state.
Understanding roles in health care through interprofessional educational experiences
Authors: Furr Susan , Lane Susan Hayes , Martin Dana , Brackney Dana E 
Published: March 2020

Background: Students can find interacting within a healthcare team challenging. It is important for students to understand their role and respect those of other healthcare team members. Interprofessional education (IPE) is a strategy for exploring the roles of self and others within the team. Aim: The purpose of this study was to evaluate nursing students' perceptions of roles and responsibilities following an IPE experience. Methods: Students in an undergraduate baccalaureate degree nursing programme participated in a two-day IPE event with students in the physician's assistant's (PA) programme, pharmacy programme, and physical therapy (PT) programme. Findings: Self-perception and the perception of others were two main themes that emerged. The results suggested that roles and responsibilities are often misunderstood. Conclusion: Educators must be committed to educating our future healthcare workforce on role expectations and responsibilities within an individual's own profession and that of others. This education should start in the foundation stages of each discipline's educational curricula.
Analisis Tipologi Potensi Pariwisata di Provinsi Jawa Tengah
Authors: Mustofa Lutfie Juliarizka , Haryati Titik 
Published: March 2020

Pariwisata merupakan salah satu bagian yang penting dari sebuah Daerah/Provinsi. Selain sebagai tempat rekreasi, pariwisata juga penting dalam 3 aspek, dari aspek ekonomi sebagai sumber pendapatan dan pajak, dari aspek sosial penciptaan lapangan kerja serta dari aspek budaya kita dapat memperkenalkan budaya kepada wistawan. Metode yang digunakan yaitu Klassen Typologi, LQ, MRP, dan Overlay. Dengan mengambil wilayah studi seluruh kabupaten/kota di Provinsi Jawa Tengah. hasil analisis dengan beberapa metode tersebut didapat hasil bahwa kabupaten Banjarnegara, Kab. Semarang, Kota Magelang, dan Kebumen merupakan kabupaten dengan pariwisata unggulan. untuk jenis wisata Alam yaitu Banjarnegara, Banyumas, Klaten, Wonosobo. Sedangkan Banjarnegara, Kab. Semarang, Kota Magelang, dan Kota Surakarta merupakan kabupaten/kota unggulan untuk jenis wisata buatan. Wisata bahari yang menjadi unggulan yaitu Brebes, Demak, Kebumen, Purworejo, Rembang, dan Kota Pekalongan. Wisata religi yang menjadi unggulan adalah Jepara dan Kab. Semarang. Kebumen, Kabupaten Magelang, Sragen, dan Wonosobo merupakan kabupaten dengan keunggulan jenis wisata kesehatannya. Wisata sejarah dan wisata budaya tidak ada yang unggul di semua kabupaten/kota. Kesimpulannya masih banyak kabupaten/kota yang belum potensial untuk itu pemerintah dapat mengembangkan sektor pariwisatanya disesuaikan dengan jenis wisatanya yang mempunyai keunggulan atau potensial untuk dikembangkan.
Tourism is one important part of a Regional / Provincial. Aside from being a place for recreation, tourism is also important in three aspects, economic aspects as a source of income and tax aspects of employment creation and social aspects of our culture can introduce the culture to wistawan. The method used is KlassenTypologi,LQ, MRP, and Overlay. By taking the entire study area districts / cities in Central Java province. the analysis results with some of these methods result is thatdistrict, Banjarnegara Kab. Semarang, Magelang, and Kebumen district is featured tourism. for this type of travel, namely Alam Banjarnegara, Banyumas, Klaten, Wonosobo. While Banjarnegara, Kab. Semarang, Magelang and Surakarta is a regency / city featured on the type of artificial travel. Marine tourism is seeded Brebes, Demak, Kebumen, Purworejo, Rembang, and Pekalongan. Religious tourism is the flagship and Kab Jepara. Semarang. Kebumen, Magelang, Sragen and Wonosobo is the district with the advantages of the type of travel health. The historical and cultural attractions nothing superior in all districts / cities. In conclusion there are many districts / cities who have the potential for the government to develop the tourism sector adapted to the type of tourism that has an advantage or potential for development.