bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased Hyaluronic Acid Level in Mid-Trimester Amniotic Fluid of Trisomy 21 Fetuses with Hydrops
Authors: Chang YL , Chao An-shine , shuenn-dyh Chang , You Shu-Han , Cheng Po-Jen 
Published: August 2020

Abstract
Background: Trisomy 21 is the most prevalent chromosomal anomaly among new-borns with mental disability. The phenotypes with trisomy 21 may involve short stature, mental retardation, shortness of the extremities, congenital heart disease and hydropsMaterial and methods: To evaluate the hyaluronic acid concentrations in amniotic fluid of trisomy 21 fetuses with and without hydrops.Materials and Methods: Totally 56 amniotic fluid supernatant samples were collected from pregnant women who underwent genetic amniocentesis for this study: group 1 were euploid fetuses without hydrops (n=30), group 2 were trisomy 21 fetuses without hydrops (n=16), group 3 were euploid fetuses with hydrops (n=6) and group 4 were trisomy 21 fetuses with hydrops (n=4). Hyaluronic acid concentrations in AF supernatants were evaluated by ELISA method.Results: There are no significant differences of hyaluronic acid concentrations between euploid and trisomy pregnancy in AF obtained from genetic amniocentesis. AF in the gestational age of amniocentesis among the four groups of fetuses; maternal age at amniotic fluid collected is oldest in group 2 fetuses. The amniotic fluid hyaluronic acid concentration is highest in group 4 fetuses, and is not significant difference among the other three groups. (P= 0.014, one way ANOVA test).Conclusion: Elevated hyaluronic acid concentration in amniotic fluid may associate with the pathophysiology of fetal hydrops in trisomy 21 fetuses.
Phosphorus Source and Availability Modulate the Rhizosphere Bacterial Community Assembly in Common Bean
Authors: Chiaramonte Josiane Barros , Vargas-Hoyos Harold Alexander , Costa Lilian Simara Abreu Soares , Rossmann Maike , Silva Daiana Alves , Bulgarelli Davide , Mendes Rodrigo 
Published: August 2020

Abstract
Background

Phosphorus (P) availability is the main nutritional factor that limits crops yields in tropical soils due to edaphic processes that lead to P immobilization after mineral fertilization. Considering the potential of the rhizosphere microbiome to transform insoluble P into forms readily available for plant uptake, in this study is proposed that plants with contrasting P uptake efficiency, growing under depleted amounts of P are able to shape distinct bacterial communities in the rhizosphere enriching taxa specialized in P mobilization.
Methods

We selected two common bean genotypes contrasting in P efficiency uptake and grew them in a soil with a gradient of two different sources of P, triple superphosphate (TSP) or rock phosphate Bayovar (RPB). The rhizosphere bacterial community was assessed by 16S rRNA amplicon sequencing. Data analyses focused in describing the structure of the bacterial communities, identification of OTUs differentially enriched in different treatments, functional metagenomic prediction and cooccurrence network.
Results

P sources and levels resulted in different rhizosphere bacterial community structure. A high number of differentially enriched OTUs were observed under P depleted conditions in the P-inefficient genotype, mainly belonging to Actinobacteria phylum. The P-inefficient genotype did not show significant differences in the rhizosphere bacterial community assembly growing in different P sources. Predicted metagenome profiles showed the enrichment of bacterial functions involved in P mobilization, in the rhizosphere of the P inefficient genotype cultivated in P depleted conditions. The network analysis revealed that in the rhizosphere of the P-inefficient genotype under P depleted conditions the bacterial community has a higher number of nodes and edges, higher average degree and clustering coeficient when compared to the treatment with optimal P level.
Conclusion

Our data showed that the uptake of exogenous input resulted in the assembly of a P-competent microbiome in the P-inefficient genotype compared to the efficient one, supporting the hypothesis that the selective pressure for the P uptake engages P-inefficient genotypes in symbiotic relationships with the soil microbiome. These results will pave the way for future experimentation aiming at explore the contribution of this P-competent microbiome to plant growth and development in a range of soil type.
Assessment of Novice Learners in a Novel High-fidelity Simulation-Based Extracorporeal Membrane Oxygenation Education Program
Authors: TONG Li , HU Wen-jie , ZHU Yan-Ping , CAO Lu , LI Ying-Hui , ZHANG Ning , CAI Chang-jie , KUANG Ming 
Published: August 2020

Abstract
Background: This study aimed to design a suitable integrated extracorporeal membrane oxygenation (ECMO) curriculum and assessed the impact of this a high-fidelity simulation-based education module on novice learners.Methods: An ECMO training curriculum was developed by well-trained and experienced ECMO experts that incorporated simulation modules to train multidisciplinary health care professionals. Pre- and post-participation questionnaires were used to determine the effects on the knowledge, ability, and confidence level of the participants. A five-point Likert scale was used to assess the participants regarding the ECMO practical test.Results: The study enrolled 37 participants (10 doctors, 27nurses). 67.6% (25/37) of trainees didn’t have ECMO experience. All two questionnaires were completed by 37 participants. A five-point Likert scale on practical test was finished by 4 ECMO experts. All participants passed the written and practical tests. All the responding participants thought the curriculum was useful in improving their perception of their overall knowledge and their ability to perform the required critical performance criteria on simulated ECMO. 91.9% (34/37) trainees thought that they invested more in active learning during the whole course; 60% (22/37) of them achieved a good level of technical evaluation. On the other hand, 67.6% (25/37) of the trainees have shown a good self-confidence in the priming course; however, 43.2% (16/27) trainees still needed to strengthen their overall competence in ECMO management. Conclusions: The integration of traditional teaching and high-fidelity simulation teaching can effectively improve the ECMO monitoring level and management skills of novice learners, but whether this training mode can be successfully transferred to the clinical field needs further research and confirmation.
Development of PREPARE (PREdicting PAtients’ Long-Term Outcome for REcovery): A Practically Usable Prediction Model for Quality of Life of ICU Survivors
Authors: Wubben Nina , Boogaard Mark van den , Ramjith Jordache , Bisschops Laurens LA , Frenzel Tim , Hoeven Johannes G van der , Zegers Marieke 
Published: August 2020

Abstract
BACKGROUND Though numbers of Intensive Care Unit (ICU) survivors have been increasing, using data on patient-reported long-term physical and mental functioning post-ICU during ICU admission is rare. Individualised information about long-term quality of life (QoL) supports ICU physicians’ decision making and empowers patients to better manage their disease during ICU admission and recovery and rehabilitation. We aimed to develop a prediction model for ICU survivors’ change in QoL one year after ICU admission. METHODS This is a sub-study of an ongoing multicenter prospective cohort study (MONITOR-IC study), in which long-term outcomes of ICU patients are measured up to five years after ICU admission. Adult patients admitted ≥12hrs to the ICU between July 2016 and January 2019 were included. Moribund patients were excluded. Multivariable linear regression and best subsets regression analysis (SRA) were used for building the prediction model. Change in QoL after one year was quantified using the EuroQol five-dimensional (EQ-5D-5L) questionnaire (Dutch range: -0.446–1), and Short-Form 36 (SF-36, range: 0-100). Models were internally validated RESULTS Data on 1308 ICU survivors was used to build the PREdicting PAtients’ long-term outcome for Recovery, PREPARE, prediction model. The best model contained 33 predictors, using the EQ-5D. Explained variance (R2) was 58.0%. Using SRA, we reduced the number of predictors to 5 (R2=55.3%): QoL before admission; sex; Clinical Frailty Scale; a cerebral embolism, occlusion, bleeding or infarction prevalent at or within one hour of admission; and having been admitted to the ICU from the operating room, from the same hospital’s nursing ward. The prediction model using the EQ-5D to quantify QoL had better predictive performance than the best SF-36 model (R2=40.6%).CONCLUSIONS We developed PREPARE, a prediction model for ICU survivors’ QoL one year after ICU admission that is practically usable due to the small number of predictors, measurable within the first 24 hours of admission. The next step is to test and evaluate the use of this prediction model in conversations between ICU physicians and patients and their families to ultimately improve patient outcomes. TRIAL REGISTRATION ClinicalTrials.gov: NCT03246334 on August 11, 2017.
Horizontal Transfer Mediated Proliferation of Antibiotic Resistance Genes in High-Fat Diet Induced Obesity Mice
Authors: Wang Rui , Chen Dawei , Wang Fang , Fan Xiuqin , Fan Chaonan , Tang Tiantian , Li Ping , Yang Mengyi , Zhao Yunfeng , Qi Kemin 
Published: August 2020

Abstract
Background

Infections caused by pathogens carrying antibiotic resistance genes (ARGs) are triggering a global health crisis. Human gut microbiota is an important reservoir of ARGs, which can not only be exchanged inside the symbiotic microbiota, but can also be horizontally transferred to opportunistic pathogens. A dysbiotic gut microbiota has been associated with obesity and other metabolic disorders, which has also been described as the most unneglected global health problem. Many studies have focused on the effects of antibiotics and other exogenous chemical on the fate of ARGs, but little is known about the effect of obesity induced dysbiotic gut microbiota on the fate of ARGs.
Results

This study addressed the significant proliferation of ARGs and the dramatical change of mobile genetic elements (MGEs) in high-fat diet induced obesity (DIO) mice, which the ermB and tnpA-03 genes mostly increased, demonstrating that DIO alone could enrich the abundance of ARGs. A clear increase of serine and tyrosine in the feces showed a significant positive correlation with the dominant genes of DIO groups (ermB and tnpA-03), suggesting that serine and tyrosine might enhance DNA transposition (tnpA-03) by nucleophilic attacking a phosphorus atom of a backbone phosphate group and ultimately significantly amplified the ARGs. Additionally, ermB has been frequently found in obesity-associated Lactobacillus, which might contribute to the sharp increase of ARGs and dramatical change of MGEs in the DIO mice. Finally, procrustes analysis showed the explanatory variables of the MGEs, the metabolites, and the microbial communities for the ARGs accounted for 94.3%, 53.4%, and 68.1%, respectively.
Conclusion

This study demonstrated that DIO significantly enhanced the abundance of ARGs and dramatically changed MGEs in mouse gut microbiota. MGEs were the most direct factor affecting ARGs, and microbiota were the main driver of the proliferation of ARGs in the DIO mice.
MicroRNA-133a-5p Promotes Angiogenesis of Endothelial Progenitor Cells via TRIM59/Id1 in Ovarian Cancer
Authors: Teng Lichen , Wu Jian , Li Huiling , Wang Huiyan , Wang Ying , Yu Ming , Zhu Yu , Su Yajuan 
Published: August 2020

Abstract
Background: Endothelial progenitor cells (EPCs) play an important role in tumor angiogenesis and growth. Our previous study has indicated that over-expressed inhibitor of DNA binding/differentiation 1 (Id1) in EPCs could promote EPCs proliferation, migration, and adhesion. In this study, we assessed the effect of MicroRNAs (miRNAs) on EPCs migration and angiogenesis and its signaling pathway in patients suffered from ovarian cancer (OC).Methods: We cultured peripheral circulating EPCs derived from 32 OC patients and 20 healthy control subjects, respectively. The miRNA profiles of EPCs in ovarian cancer patients were compared with that in healthy control subjects, and aberrantly expressed miRNAs in both groups were identified via miRNA microarray and clustering analysis. Among these miRNAs, miR-133a-5p was considered as one of the most important miRNAs, which biological function in EPCs has been investigated. Bioinformatic analysis combined with knockdown and overexpression of miR-133a-5p were used to identify its target protein.Results: An obviously downregulated expression level of miR-133a-5p has been seen in EPCs with ovarian cancer patients. Downregulated expression level of miR-133a-5p has been seen in ovarian cancer tissues and ovarian cancer cells (SKOV-3 and OVCAR-3). Downregulated of miR-133a-5p can increase TRIM59 expression, moreover, downregulated of miR-133a-5p further induce migration and angiogenesis via increase VEGF and Id1 in EPCs. MiR-133a-5p pro-angiogenesis would be diminished by TRIM59 knockdown. Additionally, increased TRIM59 also can promote EPCs migration and angiogenesis.Conclusions: The study found that miR-133a-5p was an important upstream factor regulated Id1/VEGF expression. Additionally, functional studies have revealed that TRIM59 was a direct target protein of miR-133a-5p, and TRIM59 silencing attenuated the role of miR-133a-5p in angiogenesis and Id1/VEGF expression. So we proposed that miR-133a-5p would be a new target for OC therapy.
Effect of EGR1-Mediated LncRNA HOTAIR on MRP1&nbsp;Gene Expression and MDR in Lung Cancer
Authors: Shao Shuli , Du Yang , Zhu Shaowei , Zhang Weiwei , Zhang Zhenzhu , Sun Yingning , Cui Tingting , Liu Xianglu , Feng Yunjianan , Liu Chang 
Published: August 2020

Abstract
Background: Multi-drug resistance-associated protein 1 (MRP1) plays critical roles in the multi-drug resistance (MDR) of cancer cells, and its expression is regulated by several transcription factors. However, the effects of EGR1 on MRP1 expression and MDR in lung cancer cells remain unknown.Methods: The expression of Transcription factors EGR1 and lncRNA HOTAIR in Non-small cell lung cancer were detected by using qRT-PCR, at the same time, Western Blot was used to detect the expression of transcription factor EGR1. MTT assay, Flow Cytometry and Observation with laser confocal microscope assay were used to explore the role of EGR1 and lncRNA HOTAIR in Non-small cell lung cancer cells. ChIP PCR assay and Luciferase reporter assays were used to demonstrate the molecular biological mechanism of EGR1 and lncRNA HOTAIR in Non-small cell lung cancer.Results: We found that EGR1 could bind to the MRP1 promoter at site -53/-42 bp and thereby regulate MRP1 expression. EGR1 knock-down reduced MRP1 expression, while EGR1 overexpression increased it. Knockdown of EGR1 increased the drug sensitivity to 5-Fluorouracil (5-FU), Toosendanin (TSN), and cisplatin (DDP), reduced the efflux ability of Rho-123, and induced apoptosis of drug-resistant lung cancer cells A549/DDP, while EGR1 overexpression had the opposite effects. Further, we demonstrated that lncRNA HOTAIR mediated the effects of EGR1 on MRP1 and MDR via sponging of miR-6807-3p. Moreover, we showed that miR-6807-3p exerts its function by targeting the EGR1 3'UTR. Conclusions: We revealed the role and molecular mechanisms of the novel HOTAIR/miR-6807-3p/EGR1 axis in the regulation of MRP1 expression and MDR in lung cancer cells. Our findings identify EGR1 as a potential biomarker and therapeutic target for MDR in human lung cancer.
In the Absence of Effective Malaria Parasite Sentinel System: A Cross-Sectional Study to Assess Prevalence of Plasmodium Spp Infection and Malaria Among Patients at Federal Medical Centre Birnin-Kebbi, Kebbi State, Nigeria.
Authors: Olaosebikan V. Oluwatosin , Alabi Olatunji , Hussaini Kabiru , Ojo A. Abiodun 
Published: August 2020

Abstract
Background: Local knowledge of the prevalence, burden and features of diseases as well as effective disease surveillance system to identifying population at risk are important at achieving malaria elimination of the National Malaria Strategic Plan (2014-2020). Methods: The study utilized a retrospective cross-sectional descriptive data collected from January to December, 2018 to determine the prevalence of malaria parasite infection and prevalent Plasmodium spp infection among different age groups and sexes among patients attending Federal Medical Centre, Birnin Kebbi, Kebbi State,Nigeria . We analysed secondary data of hospital records of 5,645 feverish patients attending general out patients’ department and medical laboratory department at the study area. Blood samples from the patients were collected and examined using thin and thick-blood smear slides technique for the presence or absence of parasites. Stained slides were examined using two levels of examination by trained microscopists and WHO certified expert microscopists as validators. Results: The prevalence of malaria parasite in the study area was 17.1%. P.falciparum was the most prevalent species of malaria parasite (99.69%) among the positive cases in the study area, while P.malariae was identified in 3 of the positive slides representing 0.31% case. Malaria positivity was highest (52.49%) among children aged 0-10years. Test of association between malaria positivity and patients’ age was statistically significant (p<0.05). Among patients who tested positive to malaria parasite, more than half (53.37%) of them were females. Conclusion: Presence of Plasmodium malariae may be indicative of trans-border transmission of the disease due to the proximity of the study area to international border and thus calls for effective malaria parasite surveillance system and further inter boarder research.
Reaction of Cotton Genotypes to Meloidogyne Incognita Race 3 and Selection Ofisolates With High Reproductive Rate in Cotton
Authors: Pires Ely , Antes Vanessa Aparecida , Domiciano Gisele Pereira , Souza Caio Felipe de Barros , Lima Edriana Araújo , Furlanetto Cleber 
Published: August 2020

Abstract
Background: Among the pathogens that reduce cotton productivity in Brazil, Meloidogyne incognita is one of the most important as it causes severe yield losses and is widespread. The most recommended methods for control of this species are the use of resistant cultivars and crop rotation systems. In Brazil, M. incognita races 3 and 4 have already been reported as cotton parasites but the race 3 is the most commonly found and widely disseminated.This work aimed at selecting virulent and aggressive populations of M. incognita race 3 for testing sources of resistance to this nematode in cotton genotypes.Results: The three isolates of M. incognita race 3 were important for screening of resistant cotton genotypes. The isolate from Umuarama was the most aggressive followed by Moreira Sales and Iporã. The genotypes CD 05-419, CD 05-945, CD 05-1087 and CD 05-1170 showed good performance against M. incognita race 3 in both greenhouse and field conditions.Conclusions: The cotton genotypes CD05-945, CD05-1170, CD05-1087 and CD05-419 will be selected for future work involving tests for resistance against other important cotton pathogens. Those genotypes can also be used as source of genes for resistance to nematodes in cotton breeding programs. The search for virulent and aggressive M. incognita isolates was very important when screening for resistance in cotton germplasm.
Effect of Crosstalk Between Th17 and Th9 Cells on the Activation of Dermal Vascular Smooth Muscle Cells in Systemic Sclerosis and Regulation of Tanshinone IIA
Authors: liu mengguo , li ming , fu wenwen 
Published: August 2020

Abstract
Background: To evaluate the effect of T helper 17 (Th17) cells and Th9 cells on the activation of dermal vascular smooth muscle cells (DVSMCs) in systemic sclerosis (SSc) and regulation of tanshinone IIA. Methods: The expression of interleukin 17 receptor (IL-17R) and interleukin 9 receptor (IL-9R) in the skin of SSc patients was assessed by immunofluorescence. The expression of IL-9 and IL-9R mRNA in peripheral blood mononuclear cells (PBMCs) of SSc patients were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The proportion of Th9 cells in PBMCs of SSc patients was sorted by flow cytometry. The effect of IL-9 on the differentiation of Th17 and IL-17 on that of Th9 was detected by flow cytometry. The proportion of Th9 and Th17 cells in SSc patients was detected by flow cytometry. The level of collagen I, III, α-SMA, IL-9R, IL-17R, JNK, P38, and ERK were analyzed using Western blot (WB).Results: Th9 cells were highly expressed in SSc. IL-9 stimulated the differentiation of immature T cells into Th17 cells. IL-17 induced the differentiation of immature T cells into Th9 cells. Tanshinone IIA inhibited the differentiation of immature T lymphocytes into Th17 and Th9. WB showed that the combined action of IL-17 and IL-9 upregulated the inflammation and proliferation of DVSMCs. Anti-IL17, anti-IL9, and tanshinone IIA inhibited the functional activation of DVSMCs.Conclusions: The current results suggested that Th17 and Th9 cells induced the activation of DVSMCs in SSc through crosstalk in vitro, and tanshinone IIA inhibited the process.
Distinct Expression and Prognostic Values of GATA Transcription Factor Family in Human Ovarian Cancer.
Authors: Zhou Quan , Huai-jie Yang , Man-Zhen Zuo , Tao Ya-ling 
Published: August 2020

Abstract
Accumulated studies have provided controversial evidences of expression patterns and prognostic value of the GATA transcription factor family in human ovarian cancer. In the present study, we accessed the distinct expression and prognostic roles of 7 individual members of GATA family in ovarian cancer (OC) patients through Oncoming analysis, CCLE analysis, the Kaplan–Meier plotter (KM plotter) database, cBioPortal and Metascape. Our results indicated that GATA1, GATA3, GATA4 and TRPS1 mRNA expression was significantly higher in OC than normal samples. High expression of GATA1, GATA2, and GATA4 were significantly correlated with better overall survival (OS), while increased GATA3 and GATA6 expression were associated with worse prognosis in OC patients. GATA1, GATA2, GATA3 and GATA6 were closely related to the diﬀerent clinicopathological features of OC. The genetic variation and interaction of the GATA family may be closely related to the pathogenesis and prognosis of OC, and the regulatory network composed of GATA family genes and their neighboring genes are mainly involved in Notch signaling pathway, Th1 and Th2 cell differentiation and Hippo signaling pathway. Our results might be beneficial for the better understanding of heterogeneity and complexity in the molecular biology of ovarian cancer, and paving a way for more accurate prediction of the prognosis of patients with OC.