bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comprehensive Transcriptome Analysis Identifies FXN and BDNF as Novel Targets of miRNAs in Friedreich’s Ataxia Patients
Authors: Misiorek Julia O. , Schreiber Anna M. , Urbanek-Trzeciak Martyna O. , Jazurek-Ciesiołka Magdalena , Hauser Lauren A. , Lynch David R. , Napierala Jill S. , Napierala Marek 
Published: April 2020

AbstractFriedreich’s ataxia (FRDA) is a genetic neurodegenerative disease that is caused by guanine-adenine-adenine (GAA) nucleotide repeat expansions in the first intron of the frataxin (FXN) gene. Although present in the intron, this mutation leads to a substantial decrease in protein expression. Currently, no effective treatment is available for FRDA, and, in addition toFXN, other targets with therapeutic potential are continuously sought. As miRNAs can regulate the expression of a broad spectrum of genes, are used as biomarkers, and can serve as therapeutic tools, we decided to identify and characterize differentially expressed miRNAs and their targets in FRDA cells compared to unaffected control (CTRL) cells. In this study, we performed an integrated miRNAseq and RNAseq analysis using the same cohort of primary FRDA and CTRL cells. The results of the transcriptome studies were supported by bioinformatic analyses and validated by qRT-PCR. miRNA interactions with target genes were assessed by luciferase assays, qRT-PCR, and immunoblotting. In silico analysis identified theFXNtranscript as a target of five miRNAs upregulated in FRDA cells. Further studies confirmed that miRNA-224-5p indeed targetsFXN,resulting in decreases in mRNA and protein levels. We also validated the ability of miRNA-10a-5p to bind and regulate the levels of brain-derived neurotrophic factor (BDNF), an important modulator of neuronal growth. We observed a significant decrease in the levels of miRNA-10a-5p and increase in the levels ofBDNFupon correction of FRDA cells via zinc-finger nuclease (ZFN)-mediated excision of expanded GAA repeats. Our comprehensive transcriptome analyses identified miRNA-224-5p and miRNA-10a-5p as negative regulators of theFXNandBDNFexpression, respectively. These results emphasize not only the importance of miRNAs in the pathogenesis of FRDA but also their potential as therapeutic targets for this disease.
Nature's Relations: Ontology, Vulnerability, Agency
Authors: Zúñiga Didier 
Published: April 2020

AbstractPolitical theory and philosophy need to widen their view of the space in which what matters politically takes place, and I suggest that integrating the conditions of sustainability of all affected—that is, all participants in nature's relations—is a necessary first step in this direction. New materialists and posthumanists have challenged how nature and politics have traditionally been construed. While acknowledging the significance of their contributions, I critically examine the ethical and political implications of their ontological project. I focus particularly on how the decentering of human agency that they advocate for raises a set of concerns that need to be addressed in developing an appropriate ecological ethics. I argue that the latter must be attuned to the vulnerability of living beings who participate in relationships that sustain life on earth. This brings me to conclude that qualitative distinctions between the worlds ofbiosandtechneare necessary. This is because we need to think critically about ways of evaluating types of relationships so that we can assess them and establish which are worth nurturing and protecting and which are not.
Explaining mass balance and retreat dichotomies at Taku and Lemon Creek Glaciers, Alaska
Authors: McNeil Christopher , O'Neel Shad , Loso Michael , Pelto Mauri , Sass Louis , Baker Emily H. , Campbell Seth 
Published: April 2020

AbstractWe reanalyzed mass balance records at Taku and Lemon Creek Glaciers to better understand the relative roles of hypsometry, local climate and dynamics as mass balance drivers. Over the 1946–2018 period, the cumulative mass balances diverged. Tidewater Taku Glacier advanced and gained mass at an average rate of +0.25 ± 0.28 m w.e. a–1, contrasting with retreat and mass loss of −0.60 ± 0.15 m w.e. a−1 at land-terminating Lemon Creek Glacier. The uniform influence of regional climate is demonstrated by strong correlations among annual mass balance and climate data. Regional warming trends forced similar statistically significant decreases in surface mass balance after 1989: −0.83 m w.e. a–1 at Taku Glacier and −0.81 m w.e. a–1 at Lemon Creek Glacier. Divergence in cumulative mass balance arises from differences in glacier hypsometry and local climate. Since 2013 negative mass balance and glacier-wide thinning prevailed at Taku Glacier. These changes initiated terminus retreat, which could increase dramatically if calving begins. The future mass balance trajectory of Taku Glacier hinges on dynamics, likely ending the historic dichotomy between these glaciers.
Neuropediatric rehabilitation for psychogenic gait disorders in children and adolescents
Authors: Siefen G. , Kirkcaldy B. , Lücke T. 
Published: April 2020

AbstractPsychogenic gait disorders in children and adolescents represent a unique neuropediatric and rehabilitative challenge. The inability to walk frequently strikes abruptly. Triggers are not easily identifiable. Patients are commonly admitted to the hospital in an emergency. In view of the dramatic clinical picture, parents are perturbed. They expect rapid diagnostic clarification and therapy. If physical, laboratory, and X-ray examinations do not disclose any significant pathological findings, parents generally insist on consulting diverse specialists and clinics. The pivotal point of the course of treatment is the parent–doctor–patient relationship. This is notably true when child psychologists or psychiatrists have to be consulted. Typical risks and decision conflicts of the rehabilitation dynamics are described – these are augmented by several case vignettes.
A health coach-led smoking cessation program for Chinese Americans at a community health center in New York City
Authors: Lau Jennifer D. , Feldman Naumi , Lee Regina , Xu Annie , Oster Ady 
Published: April 2020

AbstractIntroductionA health coach-led smoking cessation program was implemented in a community of immigrant Chinese Americans. Follow-up was provided face-to-face or over-the-phone to provide support and address barriers. Free nicotine replacement treatment was provided for eligible participants.AimThe aim is to assess smoking cessation outcome by program components.MethodsQuitting was defined as self-reported smoking abstention for at least 3 months. Factors contributing to successful cessation were evaluated using chi-squared tests and regression analysis. Participants were randomly surveyed to measure the helpfulness of and satisfaction with the program.Results/FindingsThe program enrolled 184 participants from November 2015 to January 2017. Participants were mostly men (89%) with a mean age of 44. An intent-to-treat analysis found that 19% quit. Phone counseling had the same success as face-to-face counseling. Each additional session attended was associated with 2.1 times the odds of quitting, P < 0.001. Among the participants who quit, 70% reported the health coach was helpful in their cessation.ConclusionsA health coach-led smoking cessation program that offered phone-based counseling was successful in reducing smoking. Future programs should consider using a health coach to reduce physician burden and phone-based counseling for difficult-to-access patients to increase program reach.
Deciding on the Future: Race, Emigration and the New Economy in Cuba
Authors: Clealand Danielle Pilar 
Published: April 2020

AbstractCuban emigration in the post-Soviet period has largely been attributed to economic motivations, but without significant racial analysis. Moreover, little is known about how black Cubans on the island think about emigration. It is therefore imperative to re-examine how blacks, once cited as the Cuban Revolution's loyalists, make decisions today about remaining in Cuba and/or pursuing economic security outside of its borders. Using original survey data of black Cubans on the island, I find that economic motivations are prominent among black Cubans, but that these motivations can be multifaceted. In a study of black Cubans and emigration, the issue of increasing racial inequality and racial exclusion has significant influence on economic opportunity, which in turn influences the desire to leave Cuba to achieve economic and professional success. The results have implications for the ways in which we analyse migration throughout the Latin American region, where race has not been factored into why people migrate.
CALL research: Where are we now?
Authors: Gillespie John 
Published: April 2020

AbstractThis article considers the current position of computer-assisted language learning (CALL) research by producing an integrative synthetic overview of all the articles published in three leading international CALL journals: ReCALL (in its 31st year of publication), the CALICO Journal (its 36th) and Computer Assisted Language Learning journal (its 32nd) over a sustained recent period: 2006–2016. They are judged sufficiently representative to enable broad trends to be detected and the sector’s strengths and weaknesses to be identified. The focus is on CALL research’s international reach, the range of topics researched and the nature of the studies themselves. The findings suggest that CALL research is growing internationally in the number of countries and researchers involved. A wide range of topics is researched, but there is a concentration of papers published on a cluster of popular areas. Consequently, fewer articles are published on a large number of CALL topics or, in some cases, rarely studied. The research methods employed are rigorous: in writing, structure, theory, literature awareness, and discussion and presentation of results, yet there are still weaknesses. Most empirical studies are small scale: based on one institution, a small cohort of students, over a short period of time and seldom followed up. Based on these findings, suggestions are made with a view to broadening and strengthening CALL research through targeting neglected strategic areas with special journal issues and conferences, and improving the quality of research projects. Key areas for future research are proposed.
Effect of temperature and moist conditions on seed dormancy cycling of two sympatric limestone species, Begonia guishanensis and Paraisometrum mileense, in southern China
Authors: Hu Xiao-Jian , Liu Cheng , Li Ai-Rong , Yang Xiang-Yun , Baskin Carol 
Published: April 2020

AbstractInformation about seed dormancy cycling and germination in relation to temperature and moisture conditions in the natural environment is important for the conservation and restoration of rare species, including Begonia guishanensis and Paraisometrum mileense, two sympatric perennial limestone (karst) species. Dry afterripening (DAR) and wet and dry (WD) cycles at 15/5 and 25/15°C as well as moist chilling (MC) at 15/5°C were used to mimic the natural environment at different times of the year. A field experiment was conducted to monitor seasonal changes in germination responses of the seeds. About 40–65% of B. guishanensis and 5% of P. mileense seeds were dormant at maturity. DAR at 25/15 and 15/5°C as well as MC and WD cycles at 15/5°C alleviated dormancy for B. guishanensis but not P. mileense, and WD cycles at 25/15°C induced a deeper conditional dormancy for both species. Seeds of B. guishanensis exhibited dormancy cycling in the field, with increased dormancy under natural WD cycles at relatively high temperatures during the transition from the dry to the wet season in April to May and decreased dormancy during the wet season from June to October. KNO3 mitigated the dormancy-inducing effect of both artificial and natural WD cycles at relatively high temperatures for B. guishanensis. The field experiment indicated that seeds of B. guishanensis may be able to form a persistent soil seed bank, while almost all seeds of P. mileense germinate at the beginning of the wet season in the field.
Reinterpreting space: mapping people and relationships in late medieval and early modern English cities using GIS
Authors: Colson Justin 
Published: April 2020

AbstractGeographical Information Systems (GIS) are becoming increasingly popular in historical research, especially in urban contexts. However, digitizing historical sources in a way that can be mapped using the Cartesian co-ordinate systems of a GIS is often challenging, especially so in the case of records pre-dating centralized property registers or street numbering. This article explores how the vernacular spatial descriptions used in several case-studies of documents from late medieval and early modern London can be translated and geocoded into GIS compatible co-ordinates in a sympathetic way. Translating this data from a historical spatial paradigm into a modern one unlocks a whole range of new insights into spatial patterns, networks and relationships which would not have been feasible to construct using traditional methods
Influence of oocyte selection, activation with a zinc chelator and inhibition of histone deacetylases on cloned porcine embryo and chemically activated oocytes development
Authors: Ongaratto Felipe L. , Rodriguez-Villamil Paula , Bertolini Marcelo , Carlson Daniel F. 
Published: April 2020

SummaryThe aim of this study was to evaluate the effects of alternative protocols to improve oocyte selection, embryo activation and genomic reprogramming on in vitro development of porcine embryos cloned by somatic cell nuclear transfer (SCNT). In Experiment 1, in vitro-matured oocytes were selected by exposure to a hyperosmotic sucrose solution prior to micromanipulation. In Experiment 2, an alternative chemical activation protocol using a zinc chelator as an adjuvant (ionomycin + N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) + N-6-dimethylaminopurine (6-DMAP)) was compared with a standard protocol (ionomycin + 6-DMAP) for the activation of porcine oocytes or SCNT embryos. In Experiment 3, presumptive cloned zygotes were incubated after chemical activation in a histone deacetylase inhibitor (Scriptaid) for 15 h, with the evaluation of embryo yield and total cell number in day 7 blastocysts. In Experiment 1, cleavage rates tended to be higher in sucrose-treated oocytes than controls (123/199, 61.8% vs. 119/222, 53.6%, respectively); however, blastocyst rates were similar between groups. In Experiment 2, cleavage rates were higher in zygotes treated with TPEN than controls but no difference in blastocyst rates between groups occurred. For Experiment 3, the exposure to Scriptaid did not improve embryo development after cloning. Nevertheless, the total number of cells was higher in cloned zygotes treated with Scriptaid than SCNT controls. In conclusion, oocyte selection by sucrose as well as treatments with zinc chelator and an inhibitor of histone deacetylases did not significantly improve blastocyst yield in cloned and parthenotes. However, the histone deacetylases inhibitor produced a significant improvement in the blastocyst quality.
Kinematic, bioenergetic and oxidative evaluations of donkey sperm preserved at +4°C
Authors: Di Palma Tommaso , Cecchini Stefano , Macchia Giuseppe , Pasolini Maria Pia , Cocchia Natascia , Boni Raffaele 
Published: April 2020

SummaryInformation on donkey sperm bioenergetics, kinetics and oxidative status is scarce even though crucial for development of reproductive technologies and germplasm conservation. For these reasons, it is interesting to monitor sperm kinetics, bioenergetics, and oxidative status during sperm storage at +4°C and with several sperm extenders and concentrations. Donkey semen was collected from three jackasses, three times each. It was diluted with four extenders (Kenney, Equiplus, INRA96 or Hippex), set at three sperm concentrations (30, 50 or 70 × 106spermatozoa/ml) and evaluated for its functionality after 0, 3, 24, 48 and 72 h storage at +4°C. Sperm kinetics was analyzed by Sperm Computer Analysis; sperm bioenergetics was assessed by mitochondrial membrane potential (MMP); sperm oxidative status was evaluated by lipid peroxidation (LPO), anti-LPO potential and nitroblue tetrazolium (NBT) assays. Incubation produced a progressive (P< 0.01) decline in sperm kinetics and MMP, whereas parameters related to oxidative status either increased (LPO, NBT) or decreased (anti-LPO). The anti-LPO potential was the index better related to sperm motility and kinetics. Extenders proved to be differently (P< 0.01) effective in preserving sperm kinetics, MMP, and oxidative status. The concentration of 30 × 106spermatozoa/ml provided an optimum preservation of sperm functions. Significant correlations emerged between most parameters examined. This study identified reference criteria for storing donkey spermatozoa at +4°C. A low sperm concentration together with a proper extender are crucial requirements for optimum sperm cryopreservation efficiency. Field trials are, however, required to validate these findings, making them operational in practice.
Maternal mental health symptoms are positively associated with child dietary diversity and meal frequency but not nutritional status in Eastern Democratic Republic of Congo
Authors: Emerson Jillian A , Caulfield Laura E , Musafiri Kishimata Espoir , Nzanzu Jean-Pierre , Doocy Shannon 
Published: April 2020

AbstractObjective:The objective was to examine the association between symptoms of maternal mental health problems and child diet and nutritional status in South Kivu, Democratic Republic of Congo.Design:Maternal depression and anxiety symptoms were measured by the Hopkins Symptom Checklist-25 (HSCL-25), and post-traumatic stress was measured by the Harvard Trauma Questionnaire (HTQ). Participants were classified as having high psychological distress if their mean item score was in the upper quartile of both measures. Dependent variables included child dietary diversity, meal frequency, height-for-age z score (HAZ), weight-for-age z score (WAZ), weight-for-height z score (WHZ), stunting and underweight. Bivariate and multivariate regression analyses were conducted.Setting:The study was nested in a larger quasi-experimental study evaluating Jenga Jamaa II, a food and nutrition assistance project in Uvira and Fizi territories.Participants:In total, 812 mother–child pairs participated. Children ranged from 2·6 to 5·6 years of age.Results:HSCL-25 (ß: 0·18, P < 0·05) and HTQ (ß: 0·19, P < 0·05) were statistically significantly associated with higher dietary diversity scores, and all maternal mental health measures were associated with higher meal frequency (HSCL-25: ß: 0·13, P = 0·001; HTQ: ß: 0·12, P = 0·001; high distress: ß: 0·15, P < 0·05) and consumption of at least three meals/snacks per day (HSCL-25: OR: 2·06, HTQ: OR: 1·93, high distress: OR: 2·68, P < 0·001 for all). No significant associations were found with HAZ, WAZ, WHZ, stunting or underweight indicators.Conclusions:More severe symptoms of maternal mental health problems were positively associated with child diet, but not anthropometry indicators. More research is needed to understand the role of maternal mental health in child feeding practices in food insecure and resource-poor settings.
Body iron and lead status in early childhood and its effects on development and cognition: a longitudinal study from urban Vellore
Authors: Koshy Beena , Srinivasan Manikandan , Zachariah Susan Mary , Karthikeyan Arun S , Roshan Reeba , Bose Anuradha , Mohan Venkata Raghava , John Sushil , Ramanujam Karthikeyan , Muliyil Jayaprakash , Kang Gagandeep 
Published: April 2020

AbstractObjective:Early childhood factors can have persisting effects on development and cognition in children. We propose to explore the trends of Fe deficiency and Pb toxicity in early childhood and their association with child development at 2 years of age and cognition at 5 years.Design:Longitudinal birth cohort study.Setting:Urban slum, Vellore, India.Participants:Children enrolled at birth were followed up regularly in the first 2 years with developmental and cognitive assessments at 2 and 5 years of age, respectively.Results:The birth cohort enrolled 251 children with 228 children followed up at 2 years and 212 at 5 years of age. Fe deficiency (ID) was highest at 15 months of age and improved subsequently at 24 months. Blood Pb levels (BLL) remained high at all age groups with an increasing trend with age; 97 % at 36 months having high BLL. Persistent high mean BLL at 15 and 24 months had negative association with both cognition and expressive language raw scores of 24 months, while high mean BLL at 15, 24 and 36 months had no significant association with any of the domains of cognition at 5 years of age. Early childhood cumulative body Fe status at 7, 15 and 24 months did not show any association with child development at 2 years, but was associated with verbal, performance and processing speed components of cognition at 5 years.Conclusions:Optimising body Fe status and limiting Pb exposure in early childhood can augment child development and school entry cognition.
When Religious Voting Becomes Volatile: The Case of Jewish Voters in Britain
Authors: Barclay Andrew 
Published: April 2020

AbstractThe political preferences of Britain's Jewish community, as well as the factors which underpin them, have been historically under-researched. This paper addresses this both empirically and conceptually. The study complements emerging evidence by showing that British Jews overwhelmingly support the Conservatives, but also employs longitudinal data for the first time to show that this association has strengthened sharply over recent election cycles. These findings highlight how existing theories of the effects of religious belonging on voting struggle to explain religious groups with volatile partisan preferences, such as British Jews. The paper therefore makes the case that the mechanism of party support should be understood differently in such cases. To this end, the findings presented here are consistent with the notion that security for Jews acts as a group utility heuristic, which leads voters to reward parties on the basis of how they are judged in prioritising security for Jews.
Opportunistic Constant Target Matching—A New Method for Satellite Intercalibration
Authors: Buehler Stefan A. , Prange Marc , Mrziglod John , John Viju O. , Burgdorf Martin , Lemke Oliver 
Published: April 2020

AbstractOpportunistic constant target matching is a new method for satellite intercalibration. It solves a long‐standing issue with the traditional simultaneous nadir overpass (SNO) method, namely, that it typically provides only data points with cold brightness temperatures for humidity sounding instruments on sun‐synchronous satellites. In the new method, a geostationary infrared sensor (SEVIRI) is used to select constant target matches for two different microwave sensors (MHS on NOAA 18 and Metop A). We discuss the main assumptions and limitations of the method and explore its statistical properties with a simple Monte Carlo simulation. The method was tested in a simple case study with real observations for this combination of satellites for MHS Channel 3 at 183 ± 1 GHz, the upper tropospheric humidity channel. For the studied 3‐month test period, real observations are found to behave consistently with the simulations, increasing our confidence that the method can be a valuable tool for intercalibration efforts. For the selected case study, the new method confirms that the bias between NOAA 18 and Metop A MHS Channel 3 is very small, with absolute value below 0.05 K.