bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salivary lactate and 8-isoprostaglandin F2α as potential non-invasive biomarkers for monitoring heart failure: a pilot study
Authors: Ghimenti Silvia , Lomonaco Tommaso , Bellagambi Francesca G. , Biagini Denise , Salvo Pietro , Trivella Maria G. , Scali Maria C. , Barletta Valentina , Marzilli Mario , Di Francesco Fabio , Errachid Abdelhamid , Fuoco Roger 
Published: May 2020

AbstractHeart failure (HF) is a cardiovascular disease affecting about 26 million people worldwide costing about $100 billons per year. HF activates several compensatory mechanisms and neurohormonal systems, so we hypothesized that the concomitant monitoring of a panel of potential biomarkers related to such conditions might help predicting HF evolution. Saliva analysis by point-of-care devices is expected to become an innovative and powerful monitoring approach since the chemical composition of saliva mirrors that of blood. The aims of this study were (i) to develop an innovative procedure combining MEPS with UHPLC-MS/MS for the simultaneous determination of 8-isoprostaglandin F2α and cortisol in saliva and (ii) to monitor lactate, uric acid, TNF-α, cortisol, α-amylase and 8-isoprostaglandin F2α concentrations in stimulated saliva samples collected from 44 HF patients during their hospitalisation due to acute HF. Limit of detection of 10 pg/mL, satisfactory recovery (95–110%), and good intra- and inter-day precisions (RSD ≤ 10%) were obtained for 8-isoprostaglandin F2α and cortisol. Salivary lactate and 8-isoprostaglandin F2α were strongly correlated with NT-proBNP. Most patients (about 70%) showed a significant decrease (a factor of 3 at least) of both lactate and 8-isoprostaglandin F2α levels at discharge, suggesting a relationship between salivary levels and improved clinical conditions during hospitalization.
Routine Karyotyping Reveals Frequent Mosaic Reciprocal Chromosome Translocations in Swine: Prevalence, Pedigree, and Litter Size
Authors: Rezaei Samira , Donaldson Brendan , Villagomez Daniel A. F. , Revay Tamas , Mary Nicolas , Grossi Daniela A. , King W. Allan 
Published: May 2020

AbstractIn the routine commercial karyotype analysis on 5,481 boars, we identified 32 carriers of mosaic reciprocal translocations, half of which were carrying a specific recurrent translocation, mos t(7;9). An additional 7 mosaic translocations were identified through lymphocyte karyotype analysis from parents and relatives of mosaic carriers (n = 45), a control group of non-carrier boars (n = 73), and a mitogen assessment study (n = 20), bringing the total number of mosaic carriers to 39 cases. Mosaic translocations in all carriers were recognized to be confined to hematopoietic cells as no translocations were identified in fibroblasts cells of the carriers. In addition, negative impact on reproduction was not observed as the fertility of the carriers and their relatives were comparable to breed averages, and cryptic mosaicism was not detected in the family tree. This paper presents the first study of mosaic reciprocal translocations identified in swine through routine screening practices on reproductively unproven breeding boars while presenting evidence that these type of chromosome abnormalities are not associated with any affected phenotype on the carrier animals. In addition, the detection of recurrent mosaic translocations in this study may emphasize the non-random nature of mosaic rearrangements in swine and the potential role of genomic elements in their formation.
Prediction of the Mortality Risk in Peritoneal Dialysis Patients using Machine Learning Models: A Nation-wide Prospective Cohort in Korea
Authors: Noh Junhyug , Yoo Kyung Don , Bae Wonho , Lee Jong Soo , Kim Kangil , Cho Jang-Hee , Lee Hajeong , Kim Dong Ki , Lim Chun Soo , Kang Shin-Wook , Kim Yong-Lim , Kim Yon Su , Kim Gunhee , Lee Jung Pyo 
Published: May 2020

AbstractHerein, we aim to assess mortality risk prediction in peritoneal dialysis patients using machine-learning algorithms for proper prognosis prediction. A total of 1,730 peritoneal dialysis patients in the CRC for ESRD prospective cohort from 2008 to 2014 were enrolled in this study. Classification algorithms were used for prediction of N-year mortality including neural network. The survival hazard ratio was presented by machine-learning algorithms using survival statistics and was compared to conventional algorithms. A survival-tree algorithm presented the most accurate prediction model and outperformed a conventional method such as Cox regression (concordance index 0.769 vs 0.745). Among various survival decision-tree models, the modified Charlson Comorbidity index (mCCI) was selected as the best predictor of mortality. If peritoneal dialysis patients with high mCCI (>4) were aged ≥70.5 years old, the survival hazard ratio was predicted as 4.61 compared to the overall study population. Among the various algorithm using longitudinal data, the AUC value of logistic regression was augmented at 0.804. In addition, the deep neural network significantly improved performance to 0.841. We propose machine learning-based final model, mCCI and age were interrelated as notable risk factors for mortality in Korean peritoneal dialysis patients.
Lithium response in bipolar disorder correlates with improved cell viability of patient derived cell lines
Authors: Paul Pradip , Iyer Shruti , Nadella Ravi Kumar , Nayak Rashmitha , Chellappa Anirudh S. , Ambardar Sheetal , Sud Reeteka , Sukumaran Salil K. , Purushottam Meera , Jain Sanjeev ,   ADBS Consortium (ADBS: The Accelerator program for Discovery in Brain disorders using Stem cells), Rao Naren P. , Narayanaswamy Janardhanan C. , Sivakumar Palanimuthu T. , Kandasamy Arun , Kesavan Muralidharan , Mehta Urvakhsh Meherwan , Venkatasubramanian Ganesan , John John P. , Mukherjee Odity , Kannan Ramakrishnan , Mehta Bhupesh , Kandavel Thennarasu , Binukumar B. , Saini Jitender , Jayarajan Deepak , Shyamsundar A. , Moirangthem Sydney , Kumar K. G. Vijay , Thirthalli Jagadisha , Chandra Prabha S. , Gangadhar Bangalore N. , Murthy Pratima , Panicker Mitradas M. , Bhalla Upinder S. , Chattarji Sumantra , Benegal Vivek , Varghese Mathew , Reddy Janardhan Y. C. , Raghu Padinjat , Rao Mahendra , Viswanath Biju 
Published: May 2020

AbstractLithium is an effective, well-established treatment for bipolar disorder (BD). However, the mechanisms of its action, and reasons for variations in clinical response, are unclear. We used neural precursor cells (NPCs) and lymphoblastoid cell lines (LCLs), from BD patients characterized for clinical response to lithium (using the “Alda scale” and “NIMH Retrospective Life chart method”), to interrogate cellular phenotypes related to both disease and clinical lithium response. NPCs from two biologically related BD patients who differed in their clinical response to lithium were compared with healthy controls. RNA-Seq and analysis, mitochondrial membrane potential (MMP), cell viability, and cell proliferation parameters were assessed, with and without in vitro lithium. These parameters were also examined in LCLs from 25 BD patients (16 lithium responders and 9 non-responders), and 12 controls. MMP was lower in both NPCs and LCLs from BD; but it was reversed with in vitro lithium only in LCLs, and this was unrelated to clinical lithium response. The higher cell proliferation observed in BD was unaffected by in vitro lithium. Cell death was greater in BD. However, LCLs from clinical lithium responders could be rescued by addition of in vitro lithium. In vitro lithium also enhanced BCL2 and GSK3B expression in these cells. Our findings indicate cellular phenotypes related to the disease (MMP, cell proliferation) in both NPCs and LCLs; and those related to clinical lithium response (cell viability, BCL2/GSK3B expression) in LCLs.
The Effects of Presleep Slow Breathing and Music Listening on Polysomnographic Sleep Measures – a pilot trial
Authors: Kuula Liisa , Halonen Risto , Kajanto Kristiina , Lipsanen Jari , Makkonen Tommi , Peltonen Miina , Pesonen Anu-Katriina 
Published: May 2020

AbstractKnowledge on efficient ways to reduce presleep arousal and, therefore, improve sleep, is scanty. We explored the effects of presleep slow breathing and music listening conditions on sleep quality and EEG power spectral density in young adults in a randomized, controlled trial with a crossover design. Participants’ (N = 20, 50% females) sleep was measured on two consecutive nights with polysomnography (40 nights), the other night serving as the control condition. The intervention condition was either a 30-minute slow breathing exercise or music listening (music by Max Richter: Sleep). The intervention and control conditions were placed in a random order. We measured heart rate variability prior to, during and after the intervention condition, and found that both interventions increased immediate heart rate variability. Music listening resulted in decreased N2 sleep, increased frontal beta1 power spectral density, and a trend towards increased N3 sleep was detected. In the slow breathing condition higher central delta power during N3 was observed. While some indices pointed to improved sleep quality in both intervention groups, neither condition had robust effects on sleep quality. These explorative findings warrant further replication in different populations.
Tuning of oxygen vacancy-induced electrical conductivity in Ti-doped hematite films and its impact on photoelectrochemical water splitting
Authors: Biswas Pranab , Ainabayev Ardak , Zhussupbekova Ainur , Jose Feljin , O’Connor Robert , Kaisha Aitkazy , Walls Brian , Shvets Igor V. 
Published: May 2020

AbstractTitanium (Ti)-doped hematite (α-Fe2O3) films were grown in oxygen-depleted condition by using the spray pyrolysis technique. The impact of post-deposition annealing in oxygen-rich condition on both the conductivity and water splitting efficiency was investigated. The X-ray diffraction pattern revealed that the films are of rhombohedral α-Fe2O3 structure and dominantly directed along (012). The as-grown films were found to be highly conductive with electrons as the majority charge carriers (n-type), a carrier concentration of 1.09×1020 cm−3, and a resistivity of 5.9×10−2 Ω-cm. The conductivity of the films were reduced upon post-deposition annealing. The origin of the conductivity was attributed firstly to Ti4+ substituting Fe3+ and secondly to the ionized oxygen vacancies (VO) in the crystal lattice of hematite. Upon annealing the samples in oxygen-rich condition, VO slowly depleted and the conductivity reduced. The photocurrent of the as-grown samples was found to be 3.4 mA/cm−2 at 1.23 V vs. RHE. The solar-to-hydrogen efficiency for the as-grown sample was calculated to be 4.18% at 1.23 V vs. RHE. The photocurrents were found to be significantly stable in aqueous environment. A linear relationship between conductivity and water-splitting efficiency was established.
Development of Social Attention and Oxytocin Levels in Maltreated Children
Authors: Suzuki Shizuka , Fujisawa Takashi X. , Sakakibara Nobuko , Fujioka Toru , Takiguchi Shinichiro , Tomoda Akemi 
Published: May 2020

AbstractChild maltreatment (CM) is a major risk factor for various psychopathologies but also adversely affects social development. Research on oxytocin (OT) is currently drawing attention as an endocrine basis for social development. In this study, we investigated the relationship between visual attention to social cues and salivary OT levels in children exposed to CM. The results revealed that the CM group had a significantly lower percentage of gaze fixation for the human face eye area and lower salivary OT levels compared to the typical development group. Moreover, a path analysis suggested that gaze fixation for the eye area was a mediator of the relationship between salivary OT levels and social-emotional problems in the CM group. These results suggest that lower endogenous OT levels in maltreated children may lead to atypical development of their visual attention to eyes as a social cue, resulting in social-emotional problems.
Common and distinct lateralised patterns of neural coupling during focused attention, open monitoring and loving kindness meditation
Authors: Yordanova Juliana , Kolev Vasil , Mauro Federica , Nicolardi Valentina , Simione Luca , Calabrese Lucia , Malinowski Peter , Raffone Antonino 
Published: May 2020

AbstractMeditation has been integrated into different therapeutic interventions. To inform the evidence-based selection of specific meditation types it is crucial to understand the neural processes associated with different meditation practices. Here we explore commonalities and differences in electroencephalographic oscillatory spatial synchronisation patterns across three important meditation types. Highly experienced meditators engaged in focused attention, open monitoring, and loving kindness meditation. Improving on previous research, our approach avoids comparisons between groups that limited previous findings, while ensuring that the meditation states are reliably established. Employing a novel measure of neural coupling – the imaginary part of EEG coherence – the study revealed that all meditation conditions displayed a common connectivity pattern that is characterised by increased connectivity of (a) broadly distributed delta networks, (b) left-hemispheric theta networks with a local integrating posterior focus, and (c) right-hemispheric alpha networks, with a local integrating parieto-occipital focus. Furthermore, each meditation state also expressed specific synchronisation patterns differentially recruiting left- or right-lateralised beta networks. These observations provide evidence that in addition to global patterns, frequency-specific inter-hemispheric asymmetry is one major feature of meditation, and that mental processes specific to each meditation type are also supported by lateralised networks from fast-frequency bands.
First report of AChE1 (G119S) mutation and multiple resistance mechanisms in Anopheles gambiae s.s. in Nigeria
Authors: Fagbohun Ifeoluwa Kayode , Idowu Emmanuel Taiwo , Otubanjo Olubunmi Adetoro , Awolola Taiwo Samson 
Published: May 2020

AbstractSusceptibility and PBO synergist bioassays were done using 3–5 days old femaleAnophelesmosquito collected from Lagos State, Nigeria with WHO test papers DDT (4%), permethrin (0.75%), Bendiocarb (1%) and PBO (4%) according to standard procedures. The activities of cytochrome P450s, glutathione S-transferase and carboxylesterases were determined using biochemical assays. The presence ofkdr-w,kdr-eandAce-1Rmutations were examined using molecular assays. Resistance to DDT and permethrin inAn gambiaes.s from the four Local Government Areas (LGAs) was recorded while suspected resistance to bendiocarb was recorded in mosquitoes from Alimosho and Kosofe LGAs. PBO synergist reduced the knockdown time and also recorded significantly (P < 0.05) higher 24 hrs percentage mortality compared to non-synergized bioassays. Increased activities of detoxifying enzymes was recorded in wild mosquito compared to the insecticides susceptible laboratory strain and this was significant (P < 0.05) in P450s, esterase α and β.Kdr-wwas detected inAn. gambiaes.s from all the LGAs,kdr-e(L1014S) was detected in Alimosho, Kosofe and Ibeju-Lekki, while theAce-1Rgene was detected in Alimosho and Kosofe. Results from this study provide evidence for resistance ofAn. gambiaefrom Lagos State to multiple classes of neurotoxic insecticides with multiple resistance mechanisms to these insecticides.
Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model
Authors: Pacia Christopher Pham , Zhu Lifei , Yang Yaoheng , Yue Yimei , Nazeri Arash , Michael Gach H. , Talcott Michael R. , Leuthardt Eric C. , Chen Hong 
Published: May 2020

AbstractAlthough blood-based liquid biopsy is a promising noninvasive technique to acquire a comprehensive molecular tumor profile by detecting cancer-specific biomarkers (e.g. DNA, RNA, and proteins), there has been limited progress for brain tumor application partially because the low permeability of the blood-brain barrier (BBB) hinders the release of tumor biomarkers. We previously demonstrated focused ultrasound-enabled liquid biopsy (FUS-LBx) that uses FUS to increase BBB permeability in murine glioblastoma models and thus enhance the release of tumor-specific biomarkers into the bloodstream. The objective of this study was to evaluate the feasibility and safety of FUS-LBx in the normal brain tissue of a porcine model. Increased BBB permeability was confirmed by the significant increase (p = 0.0053) in Ktrans (the transfer coefficient from blood to brain extravascular extracellular space) when comparing the FUS-sonicated brain area with the contralateral non-sonicated area. Meanwhile, there was a significant increase in the blood concentrations of glial fibrillary acidic protein (GFAP, p = 0.0074) and myelin basic protein (MBP, p = 0.0039) after FUS sonication as compared with before FUS. There was no detectable tissue damage by T2*-weighted MRI and histological analysis. Findings from this study suggest that FUS-LBx is a promising technique for noninvasive and localized diagnosis of the molecular profiles of brain diseases with the potential to translate to the clinic.
The alleviating effect of exogenous polyamines on heat stress susceptibility of different heat resistant wheat (Triticum aestivum L.) varieties
Authors: Jing Jianguo , Guo Suyan , Li Youfang , Li Weihua 
Published: May 2020

AbstractHigh temperature inhibits wheat grain filling. Polyamines (PAs) are closely associated with plant resistance caused by abiotic stress. However, little is known about the effect of PAs on the grain filling of wheat under heat stress. Two wheat varieties differing in heat resistance were used, and endogenous PAs levels were measured during grain filling under normal growth conditions outside the greenhouse (CK), artificially simulated high temperature (HT), artificially simulated high temperature plus exogenous application of spermine (HT + Spm) and artificially simulated high temperature plus spermidine (HT + Spd) treatments. Additionally, the variation of antioxidant enzymatic activities and osmotic adjustable substances content in grains was measured during grain filling. The results showed that compared with HT,HT + Spm and HT + Spd significantly increased grain weight of XC 6 (heat-resistant variety) by 19% and 5%, and XC 31 (heat-sensitive variety) by 31% and 34%, activity of superoxide dismutase (SOD), peroxidase (POD)and catalase (CAT) and content of Spm, Spd, and proline (Pro) increased significantly, while putrescine (Put), malondialdehyde (MDA) and soluble sugar (SS)contentdecreased during grain filling; The correlation analysis showed that grain weight was negatively correlated with the content of PUT, MDA, Pro and activity of SOD and CAT and positively correlated with the content of Spd and activity of POD in grains. Our results indicated that exogenous Spm and Spd could alleviate the heat injury of grain filling.
Human platelet antigen 1-6, 9 and 15 in the Iranian population: An anthropological genetic analysis
Authors: Kazemi Mohammad Hossein , Malakootikhah Farideh , Momeni-Varposhti Zahra , Falak Reza , Delbandi Ali-Akbar , Tajik Nader 
Published: May 2020

AbstractHuman platelet antigens (HPAs) are membranous glycoproteins considered as alloantigens due to their polymorphisms. HPA-incompatibility in multiple pregnancies or blood transfusion can induce the development of alloantibodies leading to thrombocytopenia. The frequency of HPAs varies among populations, so that deep knowledge of HPA frequencies will help us to reduce those incompatibilities. Herein, we studied the allele and genotype frequencies of HPA1-6, HPA9, and HPA15 among the Iranians with intra- and inter-populations analyses on 36 worldwide populations with diverse ethnicities. The analysis shows that the HPA2 and HPA5 have the greatest differences in genotype distribution between the Iranians and other nations, although similar to other populations, the sole allele found in HPA4, 6, and 9 is “a”. Despite other HPAs, the most frequent allele in HPA15 is “b”, which is also abundant in HPA3. Hierarchical clustering indicates the highest degree of global similarity in HPA genotype frequency among Iranian, Argentinian, Brazilian, and German Turkish populations. Our findings can be applied to decrease the risk of alloimmunizations and platelet disorders, especially in neonates.
Double hemispheric Microdialysis study in poor-grade SAH patients
Authors: Torné Ramon , Culebras Diego , Sanchez-Etayo Gerard , García-García Sergio , Muñoz Guido , Llull Laura , Amaro Sergio , Heering Christian , Blasco Jordi , Zavala Elizabeth , Enseñat Joaquim 
Published: May 2020

AbstractDelayed cerebral ischemia (DCI) is a dreadful complication present in 30% of subarachnoid hemorrhage (SAH) patients. DCI prediction and prevention are burdensome in poor grade SAH patients (WFNS 4–5). Therefore, defining an optimal neuromonitoring strategy might be cumbersome. Cerebral microdialysis (CMD) offers near-real-time regional metabolic data of the surrounding brain. However, unilateral neuromonitoring strategies obviate the diffuse repercussions of SAH. To assess the utility, indications and therapeutic implications of bilateral CMD in poor grade SAH patients. Poor grade SAH patients eligible for multimodal neuromonitoring were prospectively collected. Aneurysm location and blood volume were assessed on initial Angio-CT scans. CMD probes were bilaterally implanted and maintained, at least, for 48 hours (h). Ischemic events were defined as a Lactate/Pyruvate ratio >40 and Glucose concentration <0.7 mmol/L. 16 patients were monitored for 1725 h, observing ischemic events during 260 h (15.1%). Simultaneous bilateral ischemic events were rare (5 h, 1.9%). The established threshold of ≥7 ischemic events displayed a specificity and sensitivity for DCI of 96.2% and 83.3%, respectively. Bilateral CMD is a safe and useful strategy to evaluate areas at risk of suffering DCI in SAH patients. Metabolic crises occur bilaterally but rarely simultaneously. Hence, unilateral neuromonitoring strategies underestimate the risk of infarction and the possibility to offset its consequences.
The teleost fish, blue gourami Trichopodus trichopterus, distinguishes the taste of chemically similar substances
Authors: Kasumyan Alexander O. , Mouromtsev Grigoryi E. 
Published: May 2020

AbstractBehavioural approaches permit studies of the functional features of animal gustatory systems at the organism level, but they are seldom used compared to molecular and electrophysiological methods. This imbalance is particularly apparent in studies on fish gustation. Consequently, our notion of taste preferences remains limited in fish, the most numerous and diverse group of vertebrates. The present study aimed to determine whether fish could distinguish the tastes of chemical substances with similar structures and configurations. We performed behavioural trials, where each test substance (L-alanine, glycine, L-cysteine and 9 of their derivatives; 0.1 M) was incorporated into agar pellets, and presented to blue gourami (Trichopodus trichopterus). We found that L-α-, L-β-, and D-α-alanine as well as L-cysteine and L-cystine had different palatabilities; and glycine, methyl-glycine, dimethyl-glycine-HCl, trimethyl-glycine, and glycyl-glycine had similar taste qualities. Results show that molecular transformation could shift the palatability of amino acids, which led to changes in the orosensory behaviour of blue gourami. The ability of fish to display different taste preferences for substances, like amino acids and their, derivetives, widely distributed among aquatic organisms, undoubtedly forms the sensory basis for selective feeding, which in turn, reduces the competition for food among sympatric species in natural waters.