bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early Clinical and Radiological outcomes of&nbsp;the New Anatomic Knee System : Minimum 3-year Follow-up.
Authors: Ryu Dong Jin , Jeon Yoon Sang , Shin Byung Ki , Na Yeop , Park Sung Jun , Kim Myung Ku 
Published: August 2020

Abstract
Background: For total knee arthroplasty (TKA), implant design and proper coverage are important for achieving patient satisfaction. An asymmetrical anatomic implant (Persona® knee system, Zimmer Biomet, Warsaw, Indiana) enables accurate coverage and minimal bone cutting. This study aimed to analyze the clinical and radiological outcomes of TKA using an asymmetrical anatomic implant over a minimum 3-year follow-up.Methods: The medical records of 94 patients who underwent 132 TKAs using the Persona® knee system, between April 2015 and January 2016 were reviewed. Pre- and post-operative clinical outcomes (assessed using the International Knee Documentation Committee (IKDC), Western Ontario and McMaster Universities Arthritis Index (WOMAC), and Knee Injury and Osteoarthritis Outcome Score (KOOS) scores, and range of motion (ROM) were reviewed. The mechanical hip-knee-ankle (HKA) axis, component overhang and underhang and presence of a radiolucent line (RLL) were evaluated. Liner thickness were also reviewed.Results: At 3-year follow-up, the mean ROM improved from 108.4° to 130.3°. The mean IKDC subjective, WOMAC and global KOOS score significantly improved from 29.2 to 70.3, from 64.2 to 11.5, and from 66.6 to 21.7, respectively (p<0.0001). The mean mechanical HKA axis indicated the correction of the malalignment (from 9.2° varus to 0.3° varus, p<0.001). The incidence rates of >3-mm bone-to-implant size mismatch were 6.8% and 8.3% for the femoral and tibial components, respectively. RLL was found in seven cases, but they were small (>2 mm) and did not progress. In 36.3% of the knees, the additional liner thickness (11 or 13mm) was used. No case of implant loosening or early failure related to the implant design occurred. Conclusion: TKA using the Persona® knee system improved all the clinical knee scores and ROM without signs of early failure. This knee system also provides more implant size options than the conventional TKA systems and facilitates ligament balancing.
Activation of metabolic and stress responses during subtoxic expression of the type I toxin hok in Erwinia amylovora
Authors: Peng Jingyu , Triplett Lindsay R. , Sundin George 
Published: August 2020

Abstract
Background
Toxin-antitoxin (TA) systems, abundant in prokaryotes, are composed of a toxin gene and its cognate antitoxin. Several toxins are implied to affect the physiological state and stress tolerance of bacteria in a population. However, the molecular targets or regulatory roles of TA systems are largely unknown.
Results
Here, we examined the physiological and transcriptomic changes of Erwinia amylovora cells expressing hok at subtoxic levels that were confirmed to confer no cell death, and at toxic levels that resulted in killing of cells. In both conditions, hok caused membrane rupture and collapse of the proton motive force in a subpopulation of E. amylovora cells. We demonstrated that induction of hok resulted in upregulation of ATP biosynthesis genes, and caused leakage of ATP from cells only at toxic levels. We showed that overexpression of the phage shock protein gene pspA largely reversed the cell death phenotype caused by high levels of hok induction. We also showed that induction of hok at a subtoxic level rendered a greater proportion of stationary phase E. amylovora cells tolerant to the antibiotic streptomycin.
Conclusions
We characterized the molecular mechanism of toxicity by high-level of hok induction and demonstrated that low-level expression of hok primes the stress responses of E. amylovora against further membrane and antibiotic stressors.
Physical mapping and InDel marker development for the restorer gene Rf2 in cytoplasmic male sterile CMS-D8 cotton
Authors: Feng Juanjuan , Zhang Xuexian , Zhang Meng , Guo Liping , QI Tingxiang , Tang Huini , Zhu Haiyong , Wang Hailin , Qiao Xiuqin , Xing Chaozhu , Wu Jianyong 
Published: August 2020

Abstract
Background
Cytoplasmic male sterile (CMS) with cytoplasm from Gossypium Trilobum (D8) fails to produce functional pollen. It is useful for commercial hybrid cotton seed production. The restore line of CMS-D8 containing Rf2 gene can restore the fertility of the corresponding sterile line. This study combined the whole genome resequencing bulked segregant analysis (BSA) with high-throughput SNP genotyping to accelerate the physical mapping of Rf2 locus in CMS-D8 cotton.
Methods
The fertility of backcross population ((sterile line × restorer line) × maintainer line) comprising of 1623 individuals was investigated in the field. The fertile pool (100 plants with fertile phenotypes, F-pool) and the sterile pool (100 plants with sterile phenotypes, S-pool) were constructed for BSA resequencing. Selection of 24 SNPs through high-throughput genotyping and development InDel markers narrow down the candidate interval. The PPR genes and restore line up-regulated genes in the candidate interval were analyzed by RT-PCR.
Results
The fertility investigation results showed that fertile and sterile separation ratio was consistent with 1:1. BSA resequencing technology, high-throughput SNP genotyping, and InDel markers were used to identify Rf2 locus on candidate interval of 1.48 Mb on Chromosome D05. Furthermore, it was quantified in this experiment that InDel markers co-segregated with Rf2 enhanced the selection of the restorer line. The qRT-PCR analysis revealed PPR family gene Gh_D05G3391 located in candidate interval had significantly lower expression than sterile and maintainer lines. In addition, utilization of previous anther RNA-Seq data of CMS-D8 identified that the expression level of Gh_D05G3374 encoding NB-ARC domain-containing disease resistance protein in restorer lines was significantly higher than that in sterile and maintainer lines.
Conclusions
This study not only enabled us to precise locate the restore gene Rf2 but also evaluated the utilization of InDel markers for marker assisted selection in CMS-D8 Rf2 cotton breeding line. The results of this study provide an important foundation for further studies on the mapping and cloning of restorer genes.
Severity of radiation pneumonitis, from clinical, dosimetric and biological features: a pilot study.
Authors: Aso Samantha , Navarro-Martin Arturo , Castillo Richard , Padrones Susana , Castillo Edward , Montes Ana , Ballarin Jose Ignacio Martinez , Cubero Noelia , Lopez Rosa , Rodriguez Laura , Palmero Ramon , Manresa Federico , Guerrero Thomas , Molina Maria 
Published: August 2020

Abstract
Background and objective: Radiation pneumonitis (RP) could be a lethal complication of lung cancer treatment. No reliable predictors of RP severity have been recognized. This prospective pilot study was performed to identify early predictors of high grade lung toxicity and to evaluate clinical, biological or dosimetric features associated with different grades of toxicity.Method: Sixteen patients with non-small cell lung cancer with indication of concurrent chemoradiotherapy using 60Gy/2Gy/fraction starting at cycle one of platinum based chemotherapy were included. Bronchoalveolar lavage (BAL), pulmonary function testing (PFT), and 18F-2-fluoro-2-deoxy-D-glucose positron-emission tomography was performed before radiotherapy (RT), after three weeks of treatment, and two months post-RT. For analysis, patients were grouped by grade (low [G1-G2] vs. high [G3-G5]). The two groups were compared to identify predictors of RP. Protein expression BAL and lung tissue metabolism was evaluated in two patients (RP-G1 vs. RP-G3). Categorical variables such as comorbidities, stages and locations were summarized as percentages. Radiation doses, pulmonary function values and time to RP were summarized by medians with ranges or as means with standard deviation. Longitudinal analysis PFT was performed by a T-test. Results: All 16 patients developed RP, as follows: G1 (5 pts; 31.3%); G2 (5 pts; 31.3%); G3 (5 pts; 31.3%); and G5 (1 pts; 6.1%). Patients with high grade RP presented significant decrease (p=0.02) in diffusing lung capacity for carbon monoxide (DLCO) after three week of RT. No correlation between dosimetric values and RP grades was observed. BAL analysis of the selected patients showed that CXCL-1, CD154, IL-1ra, IL-23, MIF, PAI-1 and IFN-g were overexpressed in the lungs of the RP-G3 patient, even before treatment. The pre-RT SUVmax value in the RP-G3 patient was non-significantly higher than in the patient with RP-G1. Conclusions: RT induces some degree of RP. Our data suggest that decrease in DLCO% is the most sensitive parameter for the early detection of RP. Moreover, we detect biological differences between the two grades of pneumonitis, highlighting the potential value of some cytokines as a prognostic marker for developing high grade lung toxicity. Further multicenter studies with larger sample size are essential to validate these findings.
MiR-489-3p reduced pancreatic cancer proliferation and metastasis by targeting PKM2 and LDHA involving glycolysis
Authors: Zhang Dan , He zhiwei , Shen Yiyi , wang Jie , Liu Tao , Jiang Jianxin 
Published: August 2020

Abstract
Background: Malignant proliferation and chemotherapy resistance are some of the causes of high mortality in pancreatic cancer. MicroRNAs have long been a hot spot in cancer research and are involved in tumor formation and metabolic stress responses. miR-489-3p is involved in inhibiting the growth of many tumors, but its relationship with the growth and metabolism of pancreatic cancer is not clear. Methods：We used RNA in situ hybridization to analyze the differential expression of miR-489-3p in pancreatic cancer tissues and adjacent tissues. The qRT-PCR experiment detected the content of miR-489-3p in pancreatic cancer cell lines and ordinary pancreatic ductal epithelial cells. Then we did experiments in vivo (subcutaneous tumor formation in nude mice) and in vitro (plate cloning, transwell, glycolysis related experiments) experiments to verify that miR-489-3p can continue the invasion and metastasis of pancreatic cancer and glucose metabolism. Furthermore, we confirmed that LDHA and PKM2 are the two targets of miR-489-3p through dual luciferase reporter gene experiments. Finally, several reply experiments were done to verify the regulation mechanism of miR-489-3p.Results: We determined that miR-489-3p is under-expressed in pancreatic cancer tissues by RNA in situ hybridization, and the function acquisition and deletion experiments and glycolysis experiments confirmed that miR-489-3p can inhibit the proliferation and invasion of Glycolysis. We then analyzed the website to find that miR-489-3p can target LDHA and PKM, and then we verified this finding with a luciferase report experiment. Therefore, we proceeded with recovery experiments on LDHA and PKM2, and concluded that miR-489-3p performs its function by targeting LDHA and PKM2. Finally, in vivo experiments confirmed that highly expressed miR-489-3p inhibited the growth of pancreatic cancer. Conclusion: In short, we identified miR-489-3p as a novel chemotherapy target for pancreatic cancer, and its diagnostic value deserves further study.
Internal Fixation of Anterior Acetabular Fractures with a Limited Pararectus Approach and the New Anatomical Plates: Preliminary Results
Authors: Yao Sheng , Chen Kaifang , Zhu Fengzhao , Liu Jia , Wang Yulong , Zeng Lian , Wan Yizhou , Qu Yanzhen , Yang Liang , Yang Xu , Guo Xiaodong 
Published: August 2020

Abstract
Background The surgical treatment of acetabular fracture has adverse outcomes and high risk, and minimally invasive method is a good way to reduce complications and improve hip joint function. This study is to investigate the treatment of certain acetabular fractures primarily involving the anterior column and quadrilateral plate using a limited pararectus approach and the new anatomical plates. Methods A consecutive cohort of 17 patients with anterior displaced acetabular fractures were managed operatively with minimally invasive approach and the new anatomical plates. Ten patients had anterior column fractures, 1 patient had anterior wall fracture, 4 patients had transverse fractures and 2 patients had anterior column with posterior hemi-transverse fractures. The inferior half of the pararectus approach was adopted to open the medial window and to access the anterior column and the quadrilateral plate. The new anatomical plates were used for internal fixation. Residual displacements were assessed on the postoperative CT scans using a standardized digital method. The surgical details, hip functional outcomes, and complications were noted. Results All of the patients were operated using the limited pararectus approach and the new anatomical plates successfully. The mean operative time and blood loss were 90.9 minutes and 334.1 ml, respectively. The average postoperative residual gap and step displacement were 2.9 mm and 0.7 mm, respectively. The radiological outcome was estimated according to the Matta score, ten of the cases were graded anatomical, six were graded imperfect, and one was graded poor. Follow up averaged 15 months. Functional outcomes were excellent for nine, good for six, and fair for two. It was noted that one case of peritoneal injury was repaired intraoperatively.Conclusions The limited pararectus approach with the advantages of more minimally invasive and less complications can be an alternative choice for simple anterior acetabular fractures especially involving the quadrilateral plate. The new anatomical plates can fit with the surface of the acetabulum, which saves the time of remodeling plates during operation and facilitate fracture reduction.
Prediction&nbsp;of&nbsp;Corona Virus Disease 2019 based on SIR model in China
Authors: mu jiangang 
Published: August 2020

Abstract
Background

From the end of 2019 to the beginning of 2020, the Corona Virus Disease 2019 epidemic occurred in Wuhan, China, and quickly spread to the whole country. We used SIR epidemic model to predict the epidemic trend in China.
Materials and Methods

Respectively fitted the Corona Virus Disease 2019 epidemic trend equations in China, Hubei, and Wuhan, predicted future trends, based on the hypothesis of the infectious disease process by the SIR model and the official announcement data of the Chinese Health Commission .
Results

There will be no new cases in the non-Hubei area nationwide after March 8; there will be no new cases in Hubei that non Wuhan after March 12; Wuhan will there be no new cases after March 22 ;
Conclusions

The epidemic will end soon in China, under the prevention and control measures are not relaxed.
Risk Factors for Adolescents' Mental Health During the COVID-19 Pandemic: A Comparison between Wuhan and Other Areas in China
Authors: Chen Shitao , Cheng Zeyuan , Wu Jing 
Published: August 2020

Abstract
Background: The outbreak of Coronavirus Disease is causing considerable acute risk to public health and might have an unanticipated impact on the mental health of children and adolescents in the long run. This study aims to understand whether there is a clinically significant difference in anxiety, depression, and parental rearing style when comparing adolescents from Wuhan and other cities in China. This study also intends to examine whether gender, grade in school, single child status, online learning participation, parents' involvement in COVID-19 related work, and parents being quarantined or infected due to the disease would lead to clinically significant differences in anxiety and depression. Beyond that, this study explored the pathways among the different variables in order to better understand how these factors play a part in impacting adolescents' mental health condition. Results: Results showed that there was a statistically significant difference in anxiety symptoms between participants who were from Wuhan compared to other areas, but not in depressive symptoms. In addition, participants’ grade level, gender, relative being infected, and study online have direct positive predictive value for depressive and anxiety symptoms, whereas location and sibling status have indirect predictive value. Having relatives who participated in COVID-19 related work had positive direct predictive value only toward depression, but not anxiety.Conclusions: This study discovered several risk factors for adolescents’ depression and anxiety during the pandemic. It also called for a greater awareness of Wuhan parents’ mental wellbeing and recommended a systematic approach for mental health prevention and intervention.
Chloroplast Genome Variation and Phylogenetic Relationships of Atractylodes species
Authors: wang yiheng , Wang Sheng , Liu Yanlei , Yuan Qingjun , Sun Jiahui , Guo Lanping 
Published: August 2020

Abstract
Atractylodes DC is the basic original plant of the widely used herbal medicines “Baizhu” and “Cangzhu” and an endemic genus in East Asia. Species within the genus have minor morphological differences, and the universal DNA barcodes cannot clearly distinguish the systemic relationship or identify the species of the genus. In this study, we sequenced the chloroplast genomes of all species of Atractylodes using high-throughput sequencing. The results indicate that the chloroplast genome of Atractylodes has a typical quadripartite structure and ranges from 152,294 bp (A. carlinoides) to 153,261 bp (A. macrocephala) in size. The genome of all species contains 113 genes, including 79 protein-coding genes, 30 transfer RNA genes and four ribosomal RNA genes. Four hotspots, rpl22-rps19-rpl2, psbM-trnD, trnR-trnT(GGU), and trnT(UGU)-trnL, and a total of 42-47 simple sequence repeats (SSR) were identified as the most promising potentially variable makers for species delimitation and population genetic studies. Phylogenetic analyses of the whole chloroplast genomes indicate that Atractylodes is a clade within the tribe Cynareae; Atractylodes species form a monophyly that clearly reflects the relationship within the genus. Our study included investigations of the sequences and structural genomic variations, phylogenetics and mutation dynamics of Atractylodes chloroplast genomes and will facilitate future studies in population genetics, taxonomy and species identification.
Analysis of Magnetic and Non Magnetic Nanoparticles With Newtonian/non-newtonian Base Fluids Over a Nonlinear Stretching Sheet
Authors: Jabeen kanwal , Mushtaq Muhamad , Akram Rana MUhammad 
Published: August 2020

Abstract
Unsteady mixed convectional boundary layer flow of Casson nanofluid having magnetic( Fe3O4) and nonmagnetic(

Al2O3) nanoparticles suspension within two different types of base fluids, water(Newtonian) and

Sodium Alginate(non-Newtonian), which incorporates viscous and ohmic dissipation effects over a permeable

nonlinear stretching sheet with magnetic field effects. Some suitable non-dimensional similarity transformations

are applied to convert the governing PDEs into a system of nonlinear coupled ODEs and then solved

by differential transformation method(DTM) association with Pade-approximation. To validate the present

results for limited cases, a comparison was made with previously existing literature and found to be in well

agreement. The impact of radiation, internal heat sink/ source, viscous and ohmic dissipation was discussed for

magnetic and non-magnetic nanofluid categories by tabular and graphical demonstrations. We have presented

the tabular results of various emerging parameters to discuss the nature of skin friction, Nusselt and Sherwood

numbers. It was also observed that performance of non-Newtonian(Sodium Alginate) fluid in heat and mass

transfer is better than Newtonian(water) based fluid but no major difference was seen in heat and mass transfer

when comparison was made with Magnetic and Non-magnetic nanoparticles
Prediction of intraperitoneal adhesions using striae gravidarum and scar characteristics in women undergoing repeated cesarean sections
Authors: Elprince Mohamed , Taha Omima Tharwat , khamees rasha , atwa khaled , gadallah Ahmed , al-okda noha , aal radwa abdel , ibrahim Mohamed , Aboelroose Ahmed , ashour osama , elgedawy asmaa , elbahie amira , ghoneim hanan , ahmed amal 
Published: August 2020

Abstract
Background: The current fact of increasing rates of cesarean deliveries is a catastrophe. Recurrent cesareans result in intraperitoneal adhesions that would lead to maternal morbidity during delivery. Great efforts are directed towards the prediction of intraperitoneal adhesions to provide the best care for laboring women. The aim of the current study was to evaluate the role of abdominal striae and cesarean scar characters in the prediction of intraperitoneal adhesions.Methods: This was a case- control study conducted in the emergency ward of the obstetrics and gynecology department of a tertiary hospital from June to December 2019. The study was carried on patients admitted to the ward fulfilling particular inclusion and exclusion criteria. The study included two groups, group one was assessed for the presence of striae, and the degree of intraperitoneal adhesions was evaluated during the current cesarean section. Group two included patients without evidence of abdominal striae. They were evaluated for the severity of adhesions also after evaluation of the previous scar. Evaluation of the striae was done using Davey's scoring system. The scar was assessed using the Vancouver Scar Scale. The modified Nair's scoring system was used to evaluate intraperitoneal adhesions.Results: The study group included 203 women, while the control group included 205 women. There were significant differences in the demographic characters of the recruited patients (p-value 0.001 for almost all variables). The mean Davey score in those with mild, moderate, and severe striae was 1.82 ± 0.39, 3.57 ± 0.5, and 6.73 ± 0.94, respectively (p-value <0.001). Higher scores for the parameters of the Vancouver scale were present in patients with severe striae (1.69 ± 1.01, 1.73 ± 0.57, 2.67 ± 1.23, and 1.35 ± 1.06 for scar vascularity, pigmentation, pliability, and height respectively with a p-value of <0.001 each). Thick intraperitoneal adhesions were noted significantly in women with severe striae [21 (43.75%), p-value <0.001)]. The Davey's and Vancouver scores showed highly significant predictive performance in the prediction of intraperitoneal adhesions (p-value < 0.001).Conclusion: Abdominal striae and cesarean scar were significant predictors for intraperitoneal adhesions.
Influence of Depression on Physical and Mental Health in Patients with Angina
Authors: Alharbi Raed Abdullah 
Published: August 2020

Abstract
Purpose: Angina is well known as chest pain caused by insufficient flow of oxygen-rich blood to the cardiac muscle. Although there are several studies showing an association between depression and angina, none of these studies investigated the influence of depression on the physical and mental health of adult patients with angina, which is the overall goal of this study.Methods: This study used a publicly open data source from the Behavioral Risk Factor Surveillance System (BRFSS), 2017, in the United States. The BRFSS questionnaire relating to health, behaviors, and social life was administered to the United States residents through a telephone interview. There were 450,013 participants in this survey. Different statistical methods, including descriptive, inferential, and predictive methods, were conducted through the Statistical Analysis System (version 9.4) to accomplish the goal of this study. Results: Of the 450,013 participants in the BRFSS 2017, 25,389 were adult patients diagnosed with angina. Of these, 22,875 have depression. This study showed that depression had a statistically significant influence on physical and mental health in adult patients with angina.Conclusion: Depression has a significant influence on physical and mental health of adult patients with angina. Depression increased the physical health problems by 61.95% and mental health problems by 33.15% in adult patients with angina.