bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prefrontal dysfunction associated with a history of suicide attempts among patients with recent onset schizophrenia
Authors: Matsuoka Jun , Koike Shinsuke , Satomura Yoshihiro , Okada Naohiro , Nishimura Yukika , Sakakibara Eisuke , Sakurada Hanako , Yamagishi Mika , Takahashi Katsuyoshi , Takayanagi Yoichiro , Kasai Kiyoto 
Published: October 2020

AbstractSuicide is a major cause of death in patients with schizophrenia, particularly among those with recent disease onset. Although brain imaging studies have identified the neuroanatomical correlates of suicidal behavior, functional brain activity correlates particularly in patients with recent-onset schizophrenia (ROSZ) remain unknown. Using near-infrared spectroscopy (NIRS) recording with a high-density coverage of the prefrontal area, we investigated whether prefrontal activity is altered in patients with ROSZ having a history of suicide attempts. A 52-channel NIRS system was used to examine hemodynamic changes in patients with ROSZ that had a history of suicide attempts (n = 24) or that lacked such a history (n = 62), and age- and sex-matched healthy controls (n = 119), during a block-design letter fluency task (LFT). Patients with a history of suicide attempts exhibited decreased activation in the right dorsolateral prefrontal cortex compared with those without such a history. Our findings indicate that specific regions of the prefrontal cortex may be associated with suicidal attempts, which may have implications for early intervention for psychosis.
Reduced monoaminergic nuclei MRI signal detectable in pre-symptomatic older adults with future memory decline
Authors: Venneri Annalena , De Marco Matteo 
Published: October 2020

AbstractEvidence from murine models and human post-mortem studies indicates that monoaminergic nuclei undergo degeneration at the pre-symptomatic stage of Alzheimer’s disease (AD). Analysing 129 datasets from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) and relying on the Clinical Dementia Rating as group-defining instrument, we hypothesised that the MRI signal of monoaminergic nuclei would be a statistically significant predictor of memory decline in participants initially recruited in ADNI as healthy adults. As opposed to a group of cognitively stable participants, participants developing memory decline had reduced signal in the ventral tegmental area at baseline, before any evidence of functional decline emerged. These findings indicate that monoaminergic degeneration predates the onset of memory decline in an AD-centred initiative, with a crucial involvement of very-early changes of a dopaminergic region. This translates into potential informative avenues for pharmacological treatment of pre-symptomatic AD.
Endophytic fungus, Chaetomium globosum, associated with marine green alga, a new source of Chrysin
Authors: Kamat Siya , Kumari Madhuree , Sajna Kuttuvan Valappil , Jayabaskaran C. 
Published: October 2020

AbstractThe marine ecosystem is an extraordinary reserve of pharmaceutically important, bioactive compounds even in this “synthetic age”. Marine algae-associated endophytic fungi have gained prominence as an important source of bioactive compounds. This study was conducted on secondary metabolites of Chaetomium globosum-associated with marine green alga Chaetomorpha media from the Konkan coastline, India. Its ethyl acetate extract (CGEE) exhibited an IC50 value of 7.9 ± 0.1 µg/mL on MCF-7 cells. CGEE exhibited G2M phase cell cycle arrest, ROS production and MMP loss in MCF-7 cells. The myco-components in CGEE contributing to the cytotoxicity were found by Gas Chromatography/Mass Spectrometry analyses. Chrysin, a dihydroxyflavone was one of the forty-six myco-components which is commonly found in honey, propolis and passionflower extracts. The compound was isolated and characterized as fungal chrysin using HPLC, UV–Vis spectroscopy, LC–MS, IR and NMR analyses by comparing with standard chrysin. The purified compound exhibited an IC50 value of 49.0 ± 0.6 µM while that of standard chrysin was 48.5 ± 1.6 µM in MCF-7 cells. It induced apoptosis, G1 phase cell cycle arrest, MMP loss, and ROS production. This is the first report of chrysin from an alternative source with opportunities for yield enhancement.
Clinical implication of oncogenic somatic mutations in early-stage cervical cancer with radical hysterectomy
Authors: Watanabe Takafumi , Nanamiya Hideaki , Kojima Manabu , Nomura Shinji , Furukawa Shigenori , Soeda Shu , Tanaka Daisuke , Isogai Takao , Imai Jun-ichi , Watanabe Shinya , Fujimori Keiya 
Published: October 2020

AbstractIt is well known that tumour initiation and progression are primarily an accumulation of genetic mutations. The mutation status of a tumour may predict prognosis and enable better selection of targeted therapies. In the current study, we analysed a total of 55 surgical tumours from stage IB-IIB cervical cancer (CC) patients who had undergone radical hysterectomy including pelvic lymphadenectomy, using a cancer panel covering 50 highly mutated tumorigenesis-related genes. In 35 patients (63.6%), a total 52 mutations were detected (58.3% in squamous cell carcinoma, 73.7% in adenocarcinoma), mostly in PIK3CA (34.5%) and KRAS and TP53 (9.1%). Being mutation-positive was significantly correlated with pelvic lymph node (PLN) metastasis (P = 0.035) and tended to have a worse overall survival (P = 0.076). In particular, in the patients with squamous cell carcinoma, there was a significant association between being mutation-positive and relapse-free survival (P = 0.041). The patients with PLN metastasis had a significantly worse overall survival than those without (P = 0.006). These results indicate that somatic mutation status is a predictive biomarker for PLN metastasis in early-stage CC, and is consequently related to poor prognosis. Therefore, comprehensive genetic mutations, rather than a single genetic mutation, should be examined widely in order to identify novel genetic indicators with clinical usefulness.
The relationship between circulating vitamin D3 and subclinical atherosclerosis in an elderly Asian population
Authors: Lu Ya-Wen , Chou Ruey-Hsing , Liu Li-Kuo , Chen Liang-Kung , Huang Po-Hsun , Lin Shing-Jong 
Published: October 2020

AbstractThe current evidence regarding the association between vitamin D deficiency and cardiovascular diseases/metabolic disorders is contradictory and inconclusive. In this large-scale observational study, we investigated the relationship between the serum 25-hydroxy vitamin D3 [25(OH)D] concentration and subclinical atherosclerosis in an elderly Asian population. In the I-Lan longitudinal study (ILAS), 1798 elderly, aged 50 and older, were enrolled. For each subject, serum 25-hydroxy vitamin D3 [25(OH)D] concentration and demographic data were recorded. The participants were divided into two groups according to their serum 25(OH)D level (sufficient, > 20 ng/mL and deficient, ≤ 20 ng/mL). Carotid intima-media thickness (cIMT) was measured at bilateral common carotid arteries. Subclinical atherosclerosis was defined as a mean cIMT > 0.81 mm. The mean subject age was 64 ± 9 years old, and 604 (33.6%) were identified as having serum 25(OH)D level ≤ 20 ng/mL. Subjects with serum 25(OH)D level ≤ 20 ng/mL were younger, more likely to be female and smoker, and had a higher incidence of hypertension, dyslipidemia, and metabolic syndrome, compared to those with serum 25(OH)D level > 20 ng/mL. Additionally, patients with serum 25(OH)D level ≤ 20 ng/mL were associated with a lower risk of subclinical atherosclerosis (crude OR: 0.63, 95% CI 0.50–0.81, p < 0.001), according to univariate analysis. However, after adjusting for gender and age, serum 25(OH)D level ≤ 20 ng/mL was not a significant risk factor for subclinical atherosclerosis. Serum 25(OH)D level ≤ 20 ng/mL was not an independent risk factor for subclinical atherosclerosis in this large elderly Asian population. Association observed in the univariate analysis may be confounded by gender or comorbidities.
Modification of large area Cu2O/CuO photocathode with CuS non-noble catalyst for improved photocurrent and stability
Authors: Panzeri G. , Cristina M. , Jagadeesh M. S. , Bussetti G. , Magagnin L. 
Published: October 2020

AbstractIn this work, a three-layered heterostructure Cu2O/CuO/CuS was obtained through a low-cost and large-area fabrication route comprising electrodeposition, thermal oxidation, and reactive annealing in a sulfur atmosphere. Morphological, microstructural, and compositional analysis (AFM, SEM, XRD, EDS, XPS) were carried out to highlight the surface modification of cuprous oxide film after oxidation and subsequent sulfurization. Impedance, voltammetric, and amperometric photoelectrochemical tests were performed on Cu2O, Cu2O/CuO, and Cu2O/CuO/CuS photocathodes in a sodium sulfate solution (pH 5), under 100 mW cm−2 AM 1.5 G illumination. A progressive improvement in terms of photocurrent and stability was observed after oxidation and sulfurization treatments, reaching a maximum of − 1.38 mA cm−2 at 0 V versus RHE for the CuS-modified Cu2O/CuO electrode, corresponding to a ~ 30% improvement. The feasibility of the proposed method was demonstrated through the fabrication of a large area photoelectrode of 10 cm2, showing no significant differences in characteristics if compared to a small area photoelectrode of 1 cm2.
Airway delivery of both a BCG prime and adenoviral boost drives CD4 and CD8 T cells into the lung tissue parenchyma
Authors: Kaveh Daryan A. , Garcia-Pelayo M. Carmen , Bull Naomi C. , Sanchez-Cordon Pedro J. , Spiropoulos John , Hogarth Philip J. 
Published: October 2020

AbstractHeterologous BCG prime-boost regimens represent a promising strategy for an urgently required improved tuberculosis vaccine. Identifying the mechanisms which underpin the enhanced protection induced by such strategies is one key aim which would significantly accelerate rational vaccine development. Experimentally, airway vaccination induces greater efficacy than parenteral delivery; in both conventional vaccination and heterologous boosting of parenteral BCG immunisation. However, the effect of delivering both the component prime and boost immunisations via the airway is not well known. Here we investigate delivery of both the BCG prime and adenovirus boost vaccination via the airway in a murine model, and demonstrate this approach may be able to improve the protective outcome over parenteral prime/airway boost. Intravascular staining of T cells in the lung revealed that the airway prime regimen induced more antigen-specific multifunctional CD4 and CD8 T cells to the lung parenchyma prior to challenge and indicated the route of both prime and boost to be critical to the location of induced resident T cells in the lung. Further, in the absence of a defined phenotype of vaccine-induced protection to tuberculosis; the magnitude and phenotype of vaccine-specific T cells in the parenchyma of the lung may provide insights into potential correlates of immunity.
Correction of water column height variation on 2D grid high-resolution seismic data using dGPS based methodology
Authors: Abegunrin Ayobami , Hepp Daniel A. , Mörz Tobias 
Published: October 2020

AbstractVariations in the physical properties of water column usually impede exact water column height correction on high-resolution seismic data, especially when the data are collected in shallow marine environments. Changes in water column properties can be attributed to variation in tides and currents, wind-generated swells, long and short amplitude wave-fronts, or variation in salinity and water temperature. Likewise, the proper motion of the vessel complicates the determinability of the water column height. This study provides a less time-consuming and precise differential Global Positioning System based methodology that can be applied to most types of high-resolution seismic data in order to significantly improve the tracking and quality of deduced geological interpretations on smaller depth scales. The methodology was tested on geophysical profiles obtained from the German sector of the North Sea. The focus here was to identify, distinguish and classify various sub-surface sedimentary structures in a stratigraphically highly complex shallow marine environment on decimeter small-scale. After applying the correction to the profiles, the sea floor, in general, occurs 1.1 to 3.4 m (mean of 2.2 m) deeper than the uncorrected profiles and is consistent with the sea floor from published tide corrected bathymetry data. The corrected seismic profiles were used in plotting the depth of the base of Holocene channel structures and to define their gradients. The applied correction methodology was also crucial in glacial and post-glacial valley features distinction, across profile correlation and establishing structural and stratigraphic framework of the study area.
Clinical and radiological characteristics and outcome of wake-up intracerebral hemorrhage
Authors: Martí-Fàbregas Joan , Delgado-Mederos Raquel , Martínez-Domeño Alejandro , Camps-Renom Pol , Guisado-Alonso Daniel , Guasch-Jiménez Marina , Marrero-González Paula , Jiménez-Xarrié Elena , Marín Rebeca , Prats-Sánchez Luis 
Published: October 2020

AbstractThere is little information on the characteristics of patients with wake-up intracerebral hemorrhage (WU-ICH). We aimed to evaluate frequency and relevant differences between WU-ICH and while-awake (WA) ICH patients. This is a retrospective study of a prospective database of consecutive patients with spontaneous ICH, who were classified as WU-ICH, WA-ICH or UO-ICH (unclear onset). We collected demographic, clinical and radiological data, prognostic and therapeutic variables, and outcome [(neurological deterioration, mortality, functional outcome (favorable when modified Rankin scale score 0–2)]. From a total of 466 patients, 98 (25.8%) were classified as UO-ICH according to the type of onset and therefore excluded. We studied 368 patients (mean age 73.9 ± 13.8, 51.4% men), and compared 95 (25.8%) WU-ICH with 273 (74.2%) WA-ICH. Patients from the WU-ICH group were significantly older than WA-ICH (76.9 ± 14.3 vs 72.8 ± 13.6, p = 0.01) but the vascular risk factors were similar. Compared to the WA-ICH group, patients from the WU-ICH group had a lower GCS score or a higher NIHSS score and a higher ICH score, and were less often admitted to a stroke unit or intensive care unit. There were no differences between groups in location, volume, rate of hematoma growth, frequency of intraventricular hemorrhage and outcome. One in five patients with spontaneous ICH are WU-ICH patients. Other than age, there are no relevant differences between WU and WA groups. Although WU-ICH is associated with worse prognostic markers vital and functional outcome is similar to WA-ICH patients.
Relationship between initial peritoneal dialysis modality and risk of peritonitis
Authors: Kokubu Maiko , Matsui Masaru , Uemura Takayuki , Morimoto Katsuhiko , Eriguchi Masahiro , Samejima Kenichi , Akai Yasuhiro , Tsuruya Kazuhiko 
Published: October 2020

AbstractPeritonitis is a critical complication of peritoneal dialysis (PD). Investigators have reported the risk of peritonitis in patients on continuous ambulatory peritoneal dialysis (CAPD) versus automated peritoneal dialysis (APD), but the available evidence is predominantly based on observational studies which failed to report on the connection type. Our understanding of the relationship between peritonitis risk and PD modality thus remained insufficient. We studied 285 participants who began PD treatment between 1997 and 2014 at three hospitals in Nara Prefecture in Japan. We matched 106 APD patients with 106 CAPD patients based on their propensity scores. The primary outcome was time to first episode of peritonitis within 3 years after PD commencement. In total, PD peritonitis occurred in 64 patients during the study period. Patients initiated on APD had a lower risk of peritonitis than did those initiated on CAPD in both the unadjusted and adjusted models. The hazard ratio (HR) and 95% confidence interval (CI) for the primary endpoint were 0.30 (0.17–0.53) in the fully adjusted model including connection type. In the matched cohort, APD patients had a significantly lower risk of peritonitis than did CAPD patients (log-rank: p < 0.001, HR 0.32, 95% CI 0.16–0.59). The weighting-adjusted analysis of the inverse probability of treatment yielded a similar result (HR 0.35, 95% CI 0.18–0.67). In conclusion, patients initiated on APD at PD commencement had a reduced risk of peritonitis compared with those initiated on CAPD, suggesting APD may be preferable for prevention of peritonitis among PD patients.
Manipulating the spectral collapse in two-photon Rabi model
Authors: Lo C. F. 
Published: October 2020

AbstractWe have investigated the eigenenergy spectrum of the two-photon Rabi model with a full quadratic coupling, particularly the special feature “spectral collapse”. The critical coupling strength is reduced by half from that of the two-photon Rabi model, implying that the spectral collapse can now occur at a more attainable value of the critical coupling. At the critical coupling some discrete eigenenergy levels still survive below the continuous energy spectrum, i.e. an incomplete spectral collapse, and the set of discrete eigenenergies has a one-to-one mapping with that of a particle of variable effective mass in a finite potential well. Since the energy difference between the two atomic levels specifies the depth of the potential well, the number of bound states available (or the extent of the “spectral collapse”) can be straightforwardly monitored. Obviously, this bears a great resemblance to the spectral collapse of the two-photon Rabi model, at least qualitatively. Moreover, since the full quadratic coupling includes an extra term proportional to the photon number operator only, our analysis indicates that one may manipulate the critical coupling of the two-photon Rabi model by incorporating an adjustable proportionality constant to this extra term.
The identification and functional analysis of CD8+PD-1+CD161+ T cells in hepatocellular carcinoma
Authors: Li Zhixuan , Zheng Bo , Qiu Xinyao , Wu Rui , Wu Tong , Yang Shuai , Zhu Yanjing , Wu Xuan , Wang Shan , Gu Ziqi , Shen Siyun , Wu Mengchao , Wang Hongyang , Chen Lei 
Published: October 2020

AbstractImmunotherapy is a powerful therapeutic strategy for end-stage hepatocellular carcinoma (HCC). It is well known that T cells, including CD8+PD-1+ T cells, play important roles involving tumor development. However, their underlying phenotypic and functional differences of T cell subsets remain unclear. We constructed single-cell immune contexture involving approximate 20,000,000 immune cells from 15 pairs of HCC tumor and non-tumor adjacent tissues and 10 blood samples (including five of HCCs and five of healthy controls) by mass cytometry. scRNA-seq and functional analysis were applied to explore the function of cells. Multi-color fluorescence staining and tissue micro-arrays were used to identify the pathological distribution of CD8+PD-1+CD161 +/− T cells and their potential clinical implication. The differential distribution of CD8+ T cells subgroups was identified in tumor and non-tumor adjacent tissues. The proportion of CD8+PD1+CD161+ T cells was significantly decreased in tumor tissues, whereas the ratio of CD8+PD1+CD161− T cells was much lower in non-tumor adjacent tissues. Diffusion analysis revealed the distinct evolutionary trajectory of CD8+PD1+CD161+ and CD8+PD1+CD161− T cells. scRNA-seq and functional study further revealed the stronger immune activity of CD8+PD1+CD161+ T cells independent of MHC class II molecules expression. Interestingly, a similar change in the ratio of CD8+CD161+/ CD8+CD161− T cells was also found in peripheral blood samples collected from HCC cases, indicating their potential usage clinically. We here identified different distribution, function, and trajectory of CD8+PD-1+CD161+ and CD8+PD-1+CD161− T cells in tumor lesions, which provided new insights for the heterogeneity of immune environment in HCCs and also shed light on the potential target for immunotherapy.
Assessment of a deep-learning system for fracture detection in musculoskeletal radiographs
Authors: Jones Rebecca M. , Sharma Anuj , Hotchkiss Robert , Sperling John W. , Hamburger Jackson , Ledig Christian , O’Toole Robert , Gardner Michael , Venkatesh Srivas , Roberts Matthew M. , Sauvestre Romain , Shatkhin Max , Gupta Anant , Chopra Sumit , Kumaravel Manickam , Daluiski Aaron , Plogger Will , Nascone Jason , Potter Hollis G. , Lindsey Robert V. 
Published: October 2020

AbstractMissed fractures are the most common diagnostic error in emergency departments and can lead to treatment delays and long-term disability. Here we show through a multi-site study that a deep-learning system can accurately identify fractures throughout the adult musculoskeletal system. This approach may have the potential to reduce future diagnostic errors in radiograph interpretation.
Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity
Authors: Ruhlandt Daja , Andresen Martin , Jensen Nickels , Gregor Ingo , Jakobs Stefan , Enderlein Jörg , Chizhik Alexey I. 
Published: October 2020

AbstractOne of the key photophysical properties of fluorescent proteins that is most difficult to measure is the quantum yield. It describes how efficiently a fluorophore converts absorbed light into fluorescence. Its measurement using conventional methods become particularly problematic when it is unknown how many of the proposedly fluorescent molecules of a sample are indeed fluorescent (for example due to incomplete maturation, or the presence of photophysical dark states). Here, we use a plasmonic nanocavity-based method to measure absolute quantum yield values of commonly used fluorescent proteins. The method is calibration-free, does not require knowledge about maturation or potential dark states, and works on minute amounts of sample. The insensitivity of the nanocavity-based method to the presence of non-luminescent species allowed us to measure precisely the quantum yield of photo-switchable proteins in their on-state and to analyze the origin of the residual fluorescence of protein ensembles switched to the dark state.
PAX6 mutation alters circadian rhythm and β cell function in mice without affecting glucose tolerance
Authors: Chhabra Nirav Florian , Amarie Oana Veronica , Wu Moya , Amend Anna-Lena , Rubey Marina , Gradinger Daniel , Irmler Martin , Beckers Johannes , Rathkolb Birgit , Wolf Eckhard , Feuchtinger Annette , Huypens Peter , Teperino Raffaele , Rozman Jan , Przemeck Gerhard K. H. , Hrabě de Angelis Martin 
Published: October 2020

AbstractThe transcription factor PAX6 is involved in the development of the eye and pancreatic islets, besides being associated with sleep–wake cycles. Here, we investigated a point mutation in the RED subdomain of PAX6, previously described in a human patient, to present a comprehensive study of a homozygous Pax6 mutation in the context of adult mammalian metabolism and circadian rhythm. Pax6Leca2 mice lack appropriate retinal structures for light perception and do not display normal daily rhythmic changes in energy metabolism. Despite β cell dysfunction and decreased insulin secretion, mutant mice have normal glucose tolerance. This is associated with reduced hepatic glucose production possibly due to altered circadian variation in expression of clock and metabolic genes, thereby evading hyperglycemia. Hence, our findings show that while the RED subdomain is important for β cell functional maturity, the Leca2 mutation impacts peripheral metabolism via loss of circadian rhythm, thus revealing pleiotropic effects of PAX6.