bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Relationship and Trends of Socio-Demographic Index, Healthy Life Expectancy and Life Expectancy in China
Authors: Miao Xinlei , Guo Xiuhua , Cai Yue , Zhang Xiang , Meng Qun 
Published: May 2020

Abstract
Background Socio-Demographic Index (SDI) is an index to evaluate social development. Healthy life expectancy (HLE/HALE) can comprehensively measure the population health. This paper analyzes the relationship and trend of Socio-Demographic Index, life expectancy (LE) and healthy life expectancy, which provides a reference for improving population health. Methods We used R Studio (version 1.2.5033) for correlation matrix analysis of SDI, HALE and LE. Meanwhile, we divided provinces into 3 regions according to the 2019 Chinese Health Statistics Yearbook and analyzed the distribution by ArcGIS 10.5 of healthy life expectancy and SDI of 31 provinces and cities in China.Results From 1990 to 2016, socio-demographic, life expectancy and healthy life expectancy showed a gradual increasing trend, and the level in eastern provinces were higher than western provinces in China. There is a correlation between socio-demographic (included its composition that education for aged 15 and older, Gross Domestic Product and total fertility rates under the age of 25), healthy life expectancy and life expectancy. Total fertility rates under the age of 25 has a negative correlation with other variables.Conclusions There is a strong positive correlation between healthy life expectancy, life expectancy and socio-demographic index. The government should consider take the implementation of personalized policies to steadily improve the development of SDI, at the same time to improve healthy life expectancy from improving maternal health and reducing infant mortality, paying attention to adult health, reducing the early death rate of chronic diseases.
Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality
Authors: Ou Zhanhui , Wen Qirong , Deng Yu , Yu Yang , Chen Zhiheng , Sun Ling 
Published: May 2020

Abstract
Purpose The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet. Methods In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC). Results Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P<0.05). However, the level of lipid ROS and iron were significantly increased (P<0.05). Besides, GSH level was reduced following treatment with CSC for 24 hours, while lipid ROS and iron levels were increased (P<0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P<0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 hours, and increased after being co-cultured with Fer-1(P<0.05). Conclusion Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affect semen quality.
EBV-positive intravascular large B-cell lymphoma of the liver: a case report and literature review
Authors: Li Qingjiao , Li Jinghe , Yang Keda , Peng Ying , Xiang Yao , Sun Shuyuan , Zeng Jian , Zhang Xin , Wang Junpu 
Published: May 2020

Abstract
Background Intravascular large B-cell lymphoma (IVLBCL) is an extremely rare subtype of diffuse large B-cell lymphoma that most commonly involves the central nervous system, skin, and bone marrow. To our knowledge, Epstein-Barr virus (EBV)-positive IVLBCL in the liver has never been reported in the literature. Case presentation We report a case of a 65-year-old Chinese man with complaint of fever for 18 days. No obvious abnormality was found by physical examination. Laboratory findings were notable for anemia, thrombocytopenia, and elevated level of serum lactate dehydrogenase. Bone marrow on smear, biopsy, and flow cytometry revealed no lymphoma. Imaging studies showed a slightly lower density lesion in the liver with high fluorodeoxyglucose uptake and hepatosplenomegaly. Percutaneous liver biopsy revealed clustering of large atypical lymphocytes within the hepatic sinusoids. Immunohistochemically, these lymphoma cells were positive for CD20, PAX-5, MUM-1, BCL-6 and CD5, but negative for CD3 and CD10. Besides, Epstein-Barr virus-encoded RNA was detected in tumor cells by in situ hybridization. BCL-2 , BCL-6 and MYC genes were intact tested by fluorescence in situ hybridization analysis. The patient was diagnosed as IVLBCL and died after 1 month of hospitalization without receiving immunochemotherapy. Conclusions IVLBCL of the liver is a highly rare lymphoma with nonspecific manifestations and dismal prognosis. Full recognition of its clinicopathological features will help to better diagnose this disease.
Very High Baseline HIV viremia impairs efficacy of NNRTI-based ART: A long-term observation in treatment-naïve patients
Authors: Chen Shuai , Han Yang , Song Xiaojing , Li Yanling , Zhu Ting , Lu Hongzhou , Tang Xiaoping , Zhang Tong , Zhao Min , He Yun , He Shenghua , Wang Min , Li Yongzhen , Huang Shaobiao , Li Yong , Liu Jing , Cao Wei , Li Taisheng 
Published: May 2020

Abstract
Background: High baseline HIV-RNA level (≥100,000 copies/ml) has been proved to be related with weaker virological response to antiretroviral therapy (ART). However, it was not completely clear whether a very high pre-therapy viral load (≥500,000 copies/ml) can further impair the virological response, especially in the long run. Methods: A retrospective study based on data from multicenter cohorts in China was performed. We enlisted untreated HIV infected adults recruited between18 and 65 years old, who received China’s first-line non-nucleoside reverse transcriptase inhibitor (NNRTI)-based regimen. All patients had baseline HIV-RNA levels over 500 copies/ml, showed good adherence, and were followed for at least 24 weeks. Virological suppression (VS) was defined as the first HIV-RNA <50 copies/ml. Virological failure was defined as any of incomplete viral suppression (HIV-RNA ≥200 copies/mL with no recorded VS within 24 weeks of treatment) and viral rebound (confirmed HIV-RNA level ≥50 copies/mL after VS). Chi-square analysis was used to compare rates of VS between different HIV-RNA strata. Kaplan–Meier analysis and Cox regression model were used to compare time to virological suppression. Logistic regression was used to evaluate odds to virological failure.Results: 758 treatment-naïve HIV patients in China were enlisted. Median follow-up time (IQR) was 144 (108-276) weeks. Most patients (68.2%) were given tenofovir disoproxil fumarate (TDF) + lamivudine (3TC) + efavirenz (EFV) regimen. By week 48, rates of VS in three groups (<100,000, 100,000-500,000 and ≥500,000 copies/ml) were 94.1%, 85.0%, and 63.8%, respectively (p<0.001). Very high baseline HIV viremia over 500,000 copies/ml were found to be independently associated with delayed VS [≥500,000 vs. <100,000, adjusted relative hazard (95% CI), 0.455 (0.32-0.65); p <0.001] as well as incomplete viral suppression [≥500,000 vs. <100,000, adjusted odds ratio (aOR) (95% CI), 6.084 (2.761-13.407); p <0.001] and viral rebound [≥500,000 vs. <100,000, aOR (95% CI), 3.671 (1.009-13.355), p =0.048].Conclusion: Very high levels of pre-treatment HIV-RNA were significantly related with delayed efficacy of NNRTI-based ART and increased risk of treatment failure. More potent initial regimens should be considered for those with very high baseline viremia.
Strengthening the clinical laboratory workforce in Cambodia; a case study of a mixed-method in-service training program to improve laboratory quality management system oversight.
Authors: Ong Siew Kim , Donovan Grant T , Ndefru Nayah , Song Sophanna , Leang Chhayheng , Sek Sophat , Noble Michael , Perrone Lucy A 
Published: May 2020

Abstract
Background: Laboratory diagnostic testing service delivery and compliance with international standards for laboratory quality are directly influenced by laboratory workforce competency. Many hospital laboratories in constrained resource settings such as Cambodia struggle to cope with the training needs of laboratory professionals in an environment of competing healthcare development priorities. Resource limited countries need an adaptable and effective approach to provide laboratory professionals with job-specific quality oversight training to ensure the accuracy, timeliness and reliability of diagnostic services. Case Presentation: Here we describe the results of an in-service training and mentoring program conducted with the Cambodia Ministry of Health at 12 tertiary level hospital laboratories to drive improvements in laboratory quality management systems toward ISO 15189 accreditation, which demonstrated significant progress between baseline and outcome audits in a concurrent study. This case study describes the program, and evaluates how the four primary activities, including actionable gap assessments and planning, centralized and in-situ training curriculum, in-person mentoring, and remote tele-mentoring via video communication technologies, contributed towards quality improvement in the participating laboratories.We evaluated participant responses to Likert scale and free response questions from program and training evaluation surveys, and we used thematic analysis to develop a model of best practices within the program’s four primary activities to inform future training approaches. Of these activities, participants agreed most highly that in-person visits and planning based on gap assessments contributed to their learning and ability to improve laboratory operations. Tele-mentoring was rated lowest by participants, who were critical of excessive group dialogue and distraction during web-conferencing; however, feedback suggests both in-person and remote mentoring contribute to continuing education, accountability to action, and peer collaboration and problem solving to improve workforce efforts toward improved quality management systems.Conclusions: We recommend here a package of in-service training activities for laboratory quality management system improvement initiatives in resource constrained settings that includes needs-based curricula and personalized action plans for participants; interactive and on-site training workshops; and in-person mentoring, complemented with well managed and regular tele-mentoring that focuses on knowledge retention, accountability to goals, and collaborative problem solving. Our model presents an adaptable approach to human resource development for quality improvement in medical laboratories.
Ileal proteomic changes associated with IL-25-mediated resistance against intestinal trematode infections
Authors: Álvarez-Izquierdo María , Esteban J. Guillermo , Muñoz-Antoli Carla , Toledo Rafael 
Published: May 2020

Abstract
Background: Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode, which has been extensively used to investigate the factors that determine the rejection of intestinal helminths. In this sense, several studies have shown that IL-25 is critical for the development of resistance against E. caproni in mice. In fact, treatment of mice with recombinant IL-25 generates resistance against primary E. caproni infection. However, the mechanisms by which IL-25 induces resistance remain unknown.Methods: To study the mechanisms responsible for resistance elicited by IL-25, we analyze the ileal proteomic changes induced by IL-25 in mice and their potential role in resistance. To this purpose, we compare the protein expression profiles in the ileum of four experimental groups of mice: naïve controls; E. caproni -infected mice; rIL-25-treated mice; and rIL-25-treated mice exposed to E. caproni metacercariae.Results: Quantitative comparison by 2D-DIGE showed significant changes in a total of 41 spots. Forty of those spots validated protein spots were identified by mass spectrometry corresponding to 24 proteins.Conclusions: The analysis of our results indicates that resistance to infectiuon is associated with the maintenance of the intestinal epithelial homeostasis and the regulation of proliferation and cell death. These results provide new insights into the proteins involved in the regulation of tissue homeostasis after intestinal infection and its transcendence in resistance.
Effects of bone marrow mononuclear cells on induction of axonal sprouting in cortico-cortical and cortico-striatal pathways in an animal model of cortical ablation.
Authors: Sampaio Maria de Fatima dos Santos , Guimarães Arthur Giraldi , Lourenço Camila da Silva , Pereira Messias Gonzaga , Coimbra Norberto Cysne 
Published: May 2020

Abstract
Objectives Many therapies have been proposed in order to investigate the mechanisms of neural repair associated with neurological diseases, including BMMC (Bone marrow Mononuclear Cells) transplantation. However, studies show that some brain injuries are less responsive to neural repair, such as, for example, cortical ablation. On the other hand, models of cortical ablation have shown functional recovery after BMMC transplantation. Thus, it is relevant to expand knowledge about BMMC, also, in the induction of neuroplasticity, in this model, considering treating a promising event for the rehabilitation of neurological diseases. Using the experimental model of cortical ablation, adult male Wistar rats, which is known to be poorly responsive to neuroplasticity, the aim of this study was to investigate the effects of BMMC on axonal sprouting induction in cortico-cortical and cortico-striatal projections. An anterograde neurotracer was used to evaluate the distribution of axonal fibers.ResultsQuantitative analyses were performed and the results showed that BMMC were not able to significantly induce axonal sprouting in the evaluated areas. Our results reinforced the idea that cortical ablation may be less responsive to neuroplasticity and the beneficial effects of BMMC therapy depend on the particularities of a neural microenvironment intrinsic to a given lesion.
The common concerns regarding the COVID-19 outbreak in Iran: Explored findings from a qualitative study
Authors: Shahabi Saeed , Sadati Ahmad Kalateh , Zarei Leila , Noroozi Masoome , Tabrizi Reza , Heydari Seyyed Taghi , Lankarani Kamran Bagheri 
Published: May 2020

Abstract
Background

The first cases of coronavirus disease 2019 (COVID-19) were reported in Iran on February 20, and then the virus spread rapidly in all parts of the country, so that over 95000 positive cases and approximately 6000 deaths have been confirmed in this region until May 1, 2020. Even if measures taken in this respect have had significant impacts on reducing the incidence rate of COVID-19, a large number of other problems have arisen. Following this situation, people have been also drawn against many concerns, especially Iranian population with their own previous socioeconomic and cultural problems. Therefore, this nation-wide project aimed to investigate common concerns regarding COVID-19 outbreak in Iran.
Methods

This qualitative study was conducted in Iran from February to March 2020 via an online open-ended questionnaire. The participants were also selected using convenience and snowball sampling methods. As well, the data collection process continued until data saturation was achieved. In addition, thematic content analysis was utilized to analysis the transcribed texts.
Results

The statements retrieved also represented the most challenging psychological stress experienced by the participants. Four themes were accordingly recognized based on the content analysis including stressful conditions, health concerns, social and political concerns, and economic concerns. Throughout the study, a major proportion of the participants commented that psychological disorders such as fear, anxiety, stress, and ennui were their main challenges regarding this pandemic. Furthermore, lack of social responsibility, worries about high-risk and susceptible groups, decreased economic power of the public, financial hardships for low-income groups, shortage of healthcare facilities, and adverse effects of disinfectants were expressed as the main concerns.
Conclusions

As a whole, it is evident that people have confronted with several challenges and need help together with effective policies and strategies during and after this pandemic to reduce their current concerns. The study findings provided a favorable ground to develop and adopt the required policies in Iran and other countries. It was concluded that creating local, national, and global solidarity in such epidemics is an inevitable necessity.
Comparative Heavy Metal Removal Efficiencies of Biosurfactants Produced by Odoribacter Splanchnicus DSM 20712, Bacterium Clone JX981747 and Soil Washing Agents
Authors: Ogbulie TOOCHUKWU Ekwutosi , Okore Chioma Chimezie , Ejele Agunna Everest 
Published: May 2020

Abstract
The application of two biosurfactants in the removal of copper, zinc, and lead from waste metal dumpsite soil with their efficiencies was compared to soil washing agents as KNO3, Ca(NO3)2 and NaOH. The test soil samples were also spiked separately with different concentrations (50 mg/L, 250 mg/L, 750 mg/L and 1500 mg/L ) of CuSO4, ZnSO4 and Pb(NO3)2. The biosurfactants used were produced by Odoribacter splanchnicus DSM 20712 (WBS1) and an unidentified bacterium clone JX981747 (CMS). Five different treatment set up comprising of different ratios (20:1, 15:1, 10:1, 5:1, and 1:1) with the soil solution constant was respectively used. The heavy metal contents were measured using Atomic Absorption Spectrophotometer and the percentage heavy metal removal efficiency was calculated. The highest concentrations of biosurfactant (20:1) at different spiked concentrations of metallic salts recorded the highest values of copper (95.47%, 95.73%, 91.69%, 78.82%); zinc (97.98%, 98.98%, 97.29%, 96.78%) and lead (97.68%, 93.09%, 88.12%, 84.98%) removal. The percentage metal removed in each treatment increased with increasing concentration of the biosurfactants and washing agents (1:1 to 20:1). The chemical structure of the two biosurfactants analyzed using Gas Chromatography Mass Spectroscopy (GC-MS) depict the major component of biosurfactants produced from Odoribacter splanchnicus DSM 20712 to be Di-n- amyl phthalate while 9, Octadecanoic acid, methyl ester was from unidentified bacterium clone JX981747. The one dimensional paper chromatography showed presence of galactose/glucose, mannose, ribose, rhamnose in the biosurfactants produced from Odoribacter splanchnicus DSM 20712 whereas the unidentified bacterium clone JX981747 produced biosurfactants that contained all sugars except mannose. The test biosurfactants studied showed high levels of copper and lead removal than zinc when compared with the test soil washing agents (KNO3), Ca(NO3)2 and NaOH used in this study. Biosurfactants have thus shown to have the ability to remove metals hence its use requires scaling up for environmental applications.
miR-301b-3p promotes lung adenocarcinoma cell proliferation, migration and invasion by targeting DLC1
Authors: Liu Haitao , Ma Xingjie , Niu Niu , Zhao Junjie , Lu Chao , Yang Fan , Qi Weibo 
Published: May 2020

Abstract
Background

miR-301b-3p has been reported to be abnormally expressed in various human cancers including lung cancer. However, the underlying role and molecular mechanisms in lung adenocarcinoma (LUAD) remain unclear. This study aimed to elucidate the underlying mechanisms of miR-301b-3p in LUAD.
Methods

Based on TCGA database, we found that miR-301b-3p was prominently up-regulated in LUAD tissues. A series of functional experiments including CCK-8 assay, colony formation assay and Transwell assay uncovered that the up-regulation of miR-301b-3p facilitated LUAD cell proliferation, migration and invasion abilities. Bioinformatics analysis and dual-luciferase reporter gene assay demonstrated that Deleted in Liver Cancer 1 (DLC1) was negatively regulated by miR-301b-3p, and it was extremely lowly expressed in LUAD tissues and cells.
Results

Rescue experiments suggested that overexpressing DLC1 restored the promoting effect of miR-301b-3p on LUAD cell proliferation, migration and invasion.
Conclusions

Taken together, our study elucidated that miR-301b-3p promoted LUAD cell proliferation, migration and invasion by targeted suppressing DLC1 expression. The discovery of the mechanism provides a novel therapeutic strategy for LUAD patients, which helps to improve the survival of patients.
A New Botox Therapy Method in Chronic Anal Fissüre
Authors: Boz Alper , Acar Hasan Zafer 
Published: May 2020

Abstract
BackgroundBotox toxin treatment(BT) is an effective method in chronic anal fissures(CAF).However, there are different suggestions regarding the application place and method of BT in the literature. Our aim in this study is to reveal the most appropriate application site and method in BT for CAFs. MethodsFor this purpose, 2 groups were randomly selected from patients who admitted to our hospital with CAF complaints. In our study, we performed the BT application in different places in different amounts in both groups. After the treatment, we evaluated the recurrence and anal incontinence scores of the patients in certain periods and compared them with each other. The follow-up period for our patients were at least 12 months. ResultsThe recurrence rate was found to be 33.3% in patients in Group A (Gp A) and 19.3% in patients in Group B (Gp B). Patients in Gp A had a statistically significant higher recurrence rate (inadequate treatment) compared to patients in Gp B at the first control, which was performed at first week after treatment(p<00.5). Anal incontinence rate 3.3% in Gp A, 6.4% in Gp B. The difference between anal incontinence rates according to Cleveland Clinic Incontinence Score (CCIS) was not statistically significant between patients with GP A and Gp B (p>0.05).Conclusion3-way BT in internal and external anal sphincters in CAFs, is an effective and low-risk treatment method.