bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variation in breast cancer grading: the effect of creating awareness through laboratory-specific and pathologist-specific feedback reports in 16 734 patients with breast cancer
Authors: van Dooijeweert Carmen , van Diest Paul J , Baas Inge O , van der Wall Elsken , Deckers Ivette A 
Published: April 2020

AimsHistological grade is widely used to guide the management of invasive breast cancer (IBC). Yet, substantial interlaboratory and intralaboratory grading variations exist in daily pathology practice. To create awareness and to facilitate quality improvement, feedback reports, containing case-mix-adjusted laboratory-specific grades benchmarked against other laboratories, were sent to the individual laboratories by 1 March 2018. We studied the effect of these feedback reports on interlaboratory grading variation up till 1 year later.MethodsOverall, 17 102 synoptic pathology reports of IBC resection specimens from 33 laboratories, obtained between 1 March 2017 and 1 March 2019 were retrieved from the Dutch Pathology Registry (PALGA). An overall deviation score (ODS), representing the sum of deviations from the grade-specific overall proportions, was calculated to compare the absolute deviation for all grades at once. Case-mix correction was performed by two multivariable logistic regression analyses, providing laboratory-specific ORs for high-grade versus low-grade IBC.ResultsAfter feedback, the overall range between laboratories decreased by 3.8%, 6.4% and 6.6% for grades I, II and III, respectively. Though the mean ODS remained similar (13.8% vs 13.7%), the maximum ODS decreased from 34.1% to 29.4%. The range of laboratory-specific ORs decreased by 21.9% for grade III versus grades I–II.ConclusionsAn encouraging decrease in grading variation of IBC was observed after laboratory-specific feedback. Nevertheless, the overall grading variation remains substantial. In view of the important role of grading in patient management, it is adamant that not only feedback should be provided on a regular basis but also other interventions, such as additional training, are required.
Between Mandate and Motivation
Authors: Well Mareike , Saerbeck Barbara , Jörgens Helge , Kolleck Nina 
Published: April 2020

Abstract
International public administrations (IPA s) play a significant role for the success of multilateral negotiations. As the bureaucratic bodies of international organizations, they are deeply involved in international negotiations, but receive little credit for their outcomes. By studying the administrative styles of the Secretariat of the UN Framework Convention on Climate Change (UNFCCC), this article arrives at an understanding of how this IPA aims at contributing to the outcome of multilateral climate change negotiations. Administrative styles can be conceived of, ideal typically, as being entrepreneurial or servant-like. The article observes that, despite a prohibitively strict mandate as a technocratic facilitator, the UNFCCC Secretariat adopts an entrepreneurial style at the policy initiation stage and a cautiously entrepreneurial style during the policy formulation phase. It has acquired a distinctive actor quality in the climate regime, motivated by a strong commitment to furthering meaningful cooperation and driving the UNFCCC process toward ambitious climate policies.
The Arctic: Press, Policy and the Arctic Council
Authors: Yermakova Yelena 
Published: April 2020

The changing situation in the Arctic due to global warming has prompted media coverage of a supposed “scramble for the Arctic,” an “Arctic boom,” or an “Arctic Bonanza.” Some even go further, deploying the rhetoric of a “New Cold War,” predicting an inevitable clash between the United States and Russia over interests in the region. The press coverage in both countries over the past decade reflects this new sensationalism. The academic literature unequivocally confirms that the press exerts substantial influence on governmental policy makers, and vice versa. However, while scholars agree that international organizations (IOs) are essential to shaping policies, the existing literature lacks research on media’s relationship with IOs, which often struggle to obtain the coverage and publicity they deserve.
The Arctic Council has provided an effective platform for constructive dialogue and decision making involving the USA and Russia. Accordingly, despite disagreements in other regions of the world, the two global powers have managed to cooperate in the Arctic – notwithstanding recent media coverage painting a different and incomplete picture. This project surveys the media coverage of the Arctic over the past decade in Russia and the USA and its correlation with the Arctic Council’s activities. The analysis draws upon two prominent news organizations in Russia (Kommersant and Izvestiya) and two in the USA (the New York Times and the Wall Street Journal), as well as the Arctic Council’s press releases from June 2006 to June 2017. The paper finds that there is a clear disconnect between media coverage of the region and the Arctic Council’s activities. It recommends that the media pay more attention to the organization, particularly since it is the only prominent platform for international cooperation in the Arctic.
Assessment of the Forecast Skill of Multiphysics and Multistochastic Methods within the GRAPES Regional Ensemble Prediction System in the East Asian Monsoon Region
Authors: Xu Zhizhen , Chen Jing , Jin Zheng , Li Hongqi , Chen Fajing 
Published: April 2020

Abstract
To more comprehensively and accurately address model uncertainties in the East Asia monsoon region, a single-physics suite, where each ensemble member uses the same set of physics parameterizations as the control member in combination with multiple stochastic schemes, is developed to investigate if the multistochastic schemes that combine different stochastic schemes together can be an alternative to a multiphysics suite, where each ensemble member uses a different set of physics parameterizations (e.g., cumulus convection, boundary layer, surface layer, microphysics, and shortwave and longwave radiation). For this purpose, two experiments are performed for a summer monsoon month over China: one with a multiphysics suite and the other with a single-physics suite combined with multistochastic schemes. Three stochastic schemes are applied: the stochastically perturbed parameterizations (SPP) scheme, consisting of temporally and spatially varying perturbations of 18 parameters in the microphysics, convection, boundary layer, and surface layer parameterization schemes; the stochastically perturbed parameterization tendencies (SPPT) scheme; and the stochastic kinetic energy backscatter (SKEB) scheme. The combination of the three stochastic schemes is compared with the multiphysics suite in the Global and Regional Assimilation and Prediction Enhanced System–Regional Ensemble Prediction System with a horizontal grid spacing of 15 km. Verification results show that, overall, a single-physics suite that combines SPP, SPPT, and SKEB outperforms the multiphysics suite in precipitation verification and verification for upper-air weather variables, 10-m zonal wind, and 2-m temperature in the East Asian monsoon region. The indication is that a single-physics suite combining SPP, SPPT, and SKEB may be an appropriate alternative to a multiphysics suite. This finding lays a foundation for the development and design of future regional and global ensembles.
Comparative transcriptomic analysis of global gene expression mediated by (p) ppGpp reveals common regulatory networks in Pseudomonas syringae
Authors: Liu Jun , Yu Menghao , Chatnaparat Tiyakhon , Lee Jae Hoon , Tian Yanli , Hu Baishi , Zhao Youfu 
Published: April 2020

AbstractBackgroundPseudomonas syringaeis an important plant pathogen, which could adapt many different environmental conditions. Under the nutrient-limited and other stress conditions,P. syringaeproduces nucleotide signal molecules, i.e., guanosine tetra/pentaphosphate ((p)ppGpp), to globally regulate gene expression. Previous studies showed that (p) ppGpp played an important role in regulating virulence factors inP. syringaepv.tomatoDC3000 (PstDC3000) andP. syringaepv.syringaeB728a (PssB728a). Here we present a comparative transcriptomic analysis to uncover the overall effects of (p)ppGpp-mediated stringent response inP. syringae.ResultsIn this study, we investigated global gene expression profiles ofPstDC3000 andPssB728a and their corresponding (p)ppGpp0mutants inhrp-inducing minimal medium (HMM) using RNA-seq. A total of 1886 and 1562 differentially expressed genes (DEGs) were uncovered between the (p)ppGpp0mutants and the wild-type inPstDC3000 andPssB728a, respectively. Comparative transcriptomics identified 1613 common DEGs, as well as 444 and 293 unique DEGs inPstDC3000 andPssB728a, respectively. Functional cluster analysis revealed that (p) ppGpp positively regulated a variety of virulence-associated genes, including type III secretion system (T3SS), type VI secretion system (T6SS), cell motility, cell division, and alginate biosynthesis, while negatively regulated multiple basic physiological processes, including DNA replication, RNA processes, nucleotide biosynthesis, fatty acid metabolism, ribosome protein biosynthesis, and amino acid metabolism in bothPstDC3000 andPssB728a. Furthermore, (p) ppGpp had divergent effects on other processes inPstDC3000 andPssB728a, including phytotoxin, nitrogen regulation and general secretion pathway (GSP).ConclusionIn this study, comparative transcriptomic analysis reveals common regulatory networks in bothPstDC3000 andPssB728a mediated by (p) ppGpp in HMM. In bothP. syringaesystems, (p) ppGpp re-allocate cellular resources by suppressing multiple basic physiological activities and enhancing virulence gene expression, suggesting a balance between growth, survival and virulence. Our research is important in that due to similar global gene expression mediated by (p) ppGpp in bothPstDC3000 andPssB728a, it is reasonable to propose that (p) ppGpp could be used as a target to develop novel control measures to fight against important plant bacterial diseases.
Effect of different drugs and drug combinations on killing stationary phase and biofilms recovered cells of Bartonella henselae in vitro
Authors: Zheng Xiaoyan , Ma Xiao , Li Tingting , Shi Wanliang , Zhang Ying 
Published: April 2020

Abstract
Background
Bartonella henselae is a Gram-negative bacterium transmitted to humans by a scratch from cat in the presence of ectoparasites. Humans infected with B. henselae can result in various clinical diseases including local lymphadenopathy and more serious systemic disease such as persistent bacteremia and endocarditis. The current treatment of persistent B. henselae infections is not very effective and remains a challenge. To find more effective treatments for persistent and biofilm Bartonella infections, in this study, we evaluated a panel of drugs and drug combinations based on the current treatment and also promising hits identified from a recent drug screen against stationary phase and biofilm recovered cells of B. henselae.

Results
We evaluated 14 antibiotics and 25 antibiotic combinations for activity against stationary phase B. henselae (all antibiotics were at 5 μg/ml) and found that ciprofloxacin, gentamicin, and nitrofurantoin were the most active agents, while clofazimine and miconazole had poor activity. Drug combinations azithromycin/ciprofloxacin, azithromycin/methylene blue, rifampin/ciprofloxacin, and rifampin/methylene blue could rapidly kill stationary phase B. henselae with no detectable CFU after 1-day exposure. Methylene blue and rifampin were the most active agents against the biofilm B. henselae after 6 days of drug exposure. Antibiotic combinations (azithromycin/ciprofloxacin, azithromycin/methylene blue, rifampin/ciprofloxacin, rifampin/methylene blue) completely eradicated the biofilm B. henselae after treatment for 6 days.

Conclusions
These findings may facilitate development of more effective treatment of persistent Bartonella infections in the future.
Comparison of overall survival on surgical resection versus transarterial chemoembolization with or without radiofrequency ablation in intermediate stage hepatocellular carcinoma: a propensity score matching analysis
Authors: Lin Chih-Wen , Chen Yaw-Sen , Lo Gin-Ho , Hsu Yao-Chun , Hsu Chia-Chang , Wu Tsung-Chin , Yeh Jen-Hao , Hsiao Pojen , Hsieh Pei-Min , Lin Hung-Yu , Shu Chih-Wen , Hung Chao-Ming 
Published: April 2020

Abstract
Background
Patients with Barcelona Clinic Liver Cancer (BCLC) stage B hepatocellular carcinoma (HCC) are recommended to undergo transcatheter arterial chemoembolization (TACE). However, TACE in combination with radiofrequency ablation (RFA) is not inferior to surgical resection (SR), and the benefits of surgical resection (SR) for BCLC stage B HCC remain unclear. Hence, this study aims to compare the impact of SR, TACE+RFA, and TACE on analyzing overall survival (OS) in BCLC stage B HCC.

Methods
Overall, 428 HCC patients were included in BCLC stage B, and their clinical data and OS were recorded. OS was analyzed by the Kaplan-Meier method and Cox regression analysis.

Results
One hundred forty (32.7%) patients received SR, 57 (13.3%) received TACE+RFA, and 231 (53.9%) received TACE. The OS was significantly higher in the SR group than that in the TACE+RFA group [hazard ratio (HR): 1.78; 95% confidence incidence (CI): 1.15–2.75, p = 0.009]. The OS was significantly higher in the SR group than that in the TACE group (HR: 3.17; 95% CI: 2.31–4.36, p < 0.0001). Moreover, the OS was significantly higher in the TACE+RFA group than that in the TACE group (HR: 1.82; 95% CI: 1.21–2.74, p = 0.004). The cumulative OS rates at 1, 3 and 5 years in the SR, TACE+RFA, and TACE groups were 89.2, 69.4 and 61.2%, 86.0, 57.9 and 38.2%, and 69.5, 37.0 and 15.2%, respectively. After propensity score matching, the SR group still had a higher OS than those of the TACE+RFA and TACE groups. The TACE+RFA group had a higher OS than that of the TACE group.

Conclusion
The SR group had higher OS than the TACE+RFA and TACE groups in BCLC stage B HCC. Furthermore, the TACE+RFA group had higher OS than the TACE group.
SRSF3 functions as an oncogene in colorectal cancer by regulating the expression of ArhGAP30
Authors: Wang Ji-Lin , Guo Chun-Rong , Sun Tian-Tian , Su Wen-Yu , Hu Qiang , Guo Fang-Fang , Liang Lun-Xi , Xu Jie , Xiong Hua , Fang Jing-Yuan 
Published: April 2020

Abstract
Background
Splicing factor SRSF3 is an oncogene and overexpressed in various kinds of cancers, however, the function and mechanism involved in colorectal cancer (CRC) remained unclear. The aim of this study was to explore the relationship between SRSF3 and carcinogenesis and progression of CRC.

Methods
The expression of SRSF3 in CRC tissues was detected by immunohistochemistry. The proliferation and invasion rate was analyzed by CCK-8 assay, colony formation assay, transwell invasion assay and xenograft experiment. The expression of selected genes was detected by western blot or real time PCR.

Results
SRSF3 is overexpressed in CRC tissues and its high expression was associated with CRC differentiation, lymph node invasion and AJCC stage. Upregulation of SRSF3 was also associated with shorter overall survival. Knockdown of SRSF3 in CRC cells activated ArhGAP30/Ace-p53 and decreased cell proliferation, migration and survival; while ectopic expression of SRSF3 attenuated ArhGAP30/Ace-p53 and increases cell proliferation, migration and survival. Targeting SRSF3 in xenograft tumors suppressed tumor progression in vivo.

Conclusions
Taken together, our data identify SRSF3 as a regulator for ArhGAP30/Ace-p53 in CRC, and highlight potential prognostic and therapeutic significance of SRSF3 in CRC.
Altered structural brain networks at term-equivalent age in preterm infants with grade 1 intraventricular hemorrhage
Authors: Cha Jong Ho , Choi Yong-Ho , Lee Jong-Min , Lee Joo Young , Park Hyun-Kyung , Kim Jinsup , Kim Il-Kewon , Lee Hyun Ju 
Published: April 2020

AbstractBackgroundPreterm infants are at risk for structural disruption of brain connectivity due to perinatal complications encountered during the fetal and neonatal periods. This study aimed to investigate the development of connectivity using diffusion tensor imaging at near-term age and the effect of grade 1 intraventricular hemorrhage on it.MethodsA total of 86 infants (55 preterm infants, 24 full-term infants) without apparent brain injury underwent diffusion magnetic resonance imaging (MRI) between 36 and 41 weeks post-menstrual age. The diffusion-MRI based connectomics were constructed from 64-segmented regions by using the Johns Hopkins University neonate atlas and were weighted with fractional anisotropy. The connectomes were quantified in the structural networks and investigated using network metrics, such as the clustering coefficient, local efficiency, characteristic path length, global efficiency, and small-worldness. We compared the differences in the brain networks of preterm infants with or without grade 1 intraventricular hemorrhage in binary and fractional anisotropy-weighted (wFA) connectomes.ResultsThe 55 preterm infants had a mean gestational age at birth of 29.3 ± 4.1 weeks and the 24 term-born infants, 38.1 ± 1.1 weeks. A total of 13 of the 55 preterm infants (23.6%) were diagnosed with grade 1 intraventricular hemorrhage. The development of connectivity of the brain network in preterm infants without intraventricular hemorrhage was comparable at near-term age to that in term infants. The preterm infants with germinal matrix hemorrhage exhibited higher clustering (0.093 ± 0.015 vs. 0.088 ± 0.007,p = 0.027) and local efficiency (0.151 ± 0.022 vs. 0.141 ± 0.010,p = 0.025), implying the potential for segregation. However, the preterm infants with intraventricular hemorrhage revealed a longer path length (0.291 ± 0.035 vs. 0.275 ± 0.019,p = 0.020) and lower global efficiency (3.998 ± 0.473 vs. 4.212 ± 0.281,p = 0.048), indicating a decreased integration in the wFA connectivity matrix than those without germinal matrix hemorrhage, after correcting for gestational age, sex, bronchopulmonary dysplasia, and age at scan.ConclusionGrade 1 intraventricular hemorrhage in preterm infants may enhance the capacity for local information transfer and the relative reinforcement of the segregation of networks at the expense of global integration capacity.
Lower bound for the blow-up time for a general nonlinear nonlocal porous medium equation under nonlinear boundary condition
Authors: Ouyang Baiping , Lin Yiwu , Liu Yan , Cai Zihan 
Published: April 2020

AbstractIn this paper, we study the blow-up phenomenon for a general nonlinear nonlocal porous medium equation in a bounded convex domain $(\varOmega\in \mathbb{R}^{n}, n\geq 3)$(Ω∈Rn,n≥3) with smooth boundary. Using the technique of a differential inequality and a Sobolev inequality, we derive the lower bound for the blow-up time under the nonlinear boundary condition if blow-up does really occur.
Nontraumatic warfarin-related intrapulmonary hemorrhage presenting as a lung mass
Authors: Tsuchida Hiroyuki , Fujikawa Ryo , Nakamura Hidenori , Nakamura Toru 
Published: April 2020

Abstract
Background
Although bleeding events are the major concern when using oral anticoagulants, intrathoracic hemorrhages due to warfarin are rare. Most cases in the literature have been related to trauma and have manifested as a hemothorax. Here we report a case of a nontraumatic hemorrhage within a pre-existing pulmonary cyst that presented as a lung mass during warfarin therapy.

Case presentation
A 75-year-old asymptomatic woman presented with a 10-cm-diameter mass on chest radiography that was not evident 6 months prior. She had been taking warfarin for paroxysmal atrial fibrillation and a transient ischemic attack. There was no history of chest trauma, warfarin overdosing, or any suspected interactions with other drugs such as nonsteroidal anti-inflammatory drugs or antibiotics. The prothrombin time/international normalized ratio(PT-INR) was prolonged at 4.73 and her hemoglobin level was 8.7 g/dl. Chest computed tomography(CT)revealed an air-fluid mass adjacent to the right upper and middle lobes with a pleural effusion. A CT scan obtained 15 years prior revealed a cyst at the corresponding site and the mass was diagnosed as a warfarin-related hemorrhage within the pre-existing pulmonary cyst. We performed a surgical resection of the cyst to prevent any worsening hemorrhage and subsequent infection. The postoperative course was uneventful and the patient was discharged on the 3rd postoperative day.

Conclusion
A warfarin-related thoracic hemorrhage, other than a hemothorax, could manifest as a pulmonary mass on radiography in patients with pre-existing pulmonary cysts. History taking especially of any anticoagulant medications and a precise assessment of the past images are crucial for a correct diagnosis. Once the intrapulmonary cystic hemorrhage becomes evident, prompt withdrawal with a reversal of warfarin and surgical resection are required to prevent a worsening hemorrhage and subsequent infection.
Entomological assessment of the risk of dengue outbreak in Abomey-Calavi Commune, Benin
Authors: Padonou Germain Gil , Ossè Razaki , Salako Albert Sourou , Aikpon Rock , Sovi Arthur , Kpanou Casimir , Sagbohan Hermann , Akadiri Yessoufou , Lamine Baba-Moussa , Akogbeto Martin C. 
Published: April 2020

Abstract
Background
In May 2019, a confirmed dengue fever case was detected at the local hospital of Abomey-Calavi Commune in southern Benin. In Benin, there remains a dearth of literature concerning the distribution and biology of Aedes aegypti, the principal vector of dengue fever. This study was initiated by the Ministry of Health to partially fill this gap. The findings allowed us to assess the arboviral transmission risk incurred by the population of Abomey-Calavi to support programmatic decision-making.

Methods
Entomological assessments were conducted in 5% of the houses, meaning 314 houses selected from 11 boroughs in Abomey-Calavi Centre district and 9 villages in Hêvié district. The surveyed breeding sites were water containers located in (domestic) and around (peri-domestic) the dwellings. When a container was positive (housing larvae), a portion of the immature population was sampled with a larval dipper and poured into labeled jars. Immatures were then reared to adulthood at the Centre de Recheche Entomologique de Cotonou (CREC) insectary. Adult mosquitoes were morphologically identified to species level by site and, a subsample of the collected Ae. aegypti mosquitoes were used for WHO susceptibility tube tests.

Results
Of the 1372 adult Aedes specimens which emerged from the collected larvae and pupae, 1356 Ae. aegypti (98.83%), 10 Ae. luteocephalus, and 4 Ae. vittatus were identified. The Breteau indices were 160.2 in Abomey-Calavi Centre and 150 in Hêvié, whereas the House indices were 58.5% and 61.6% in the respective districts. WHO insecticide susceptibility tube tests showed that the mortality rates were 38.71% in Abomey-Calavi Centre and 85.71% in Hêvié for permethrin, and 72.22% in Abomey-Calavi Centre and 100% in Hêvié for deltamethrin.

Conclusion
The two districts were highly infested by Ae. aegypti whose breeding sites were mostly man-made. Considering this, human behavioral change to substantially reduce the number of larval habitats is necessary to control the vector populations. As Aedes mosquitoes are day biters, the use of repellents such as ointments and smoke coils can also be useful.