bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polysaccharides extracted fromRheum tanguticumameliorate radiation-induced enteritis via activation of Nrf2/HO-1
Authors: Zhang Tian , Shi Lei , Li Yan , Mu Wei , Zhang HaoMeng , Li Yang , Wang XiaoYan , Zhao WeiHe , Qi YuHong , Liu Linna 
Published: September 2020

ABSTRACTRadiation-induced enteritis is a major side effect in cancer patients undergoing abdominopelvic radiotherapy. The Nrf2/HO-1 pathway is a critical endogenous antioxidant stress pathway, but its precise role in radiation-induced enteritis remains to be clarified. Polysaccharides extracted from Rheum tanguticum (RTP) can protect the intestinal cells from radiation-induced damage, but the underlying mechanism is unknown. SD rats and IEC-6 cells were exposed to 12 or 10 Gy X-ray radiation. Rat survival, and histopathological and immunohistochemical profiles were analyzed at different time points. Indicators of oxidative stress and inflammatory response were also assessed. Cell viability, apoptosis and Nrf2/HO-1 expression were evaluated at multiple time points. Significant changes were observed in the physiological and biochemical indexes of rats after radiation, accompanied by significant oxidative stress response. The mRNA and protein expression of Nrf2 peaked at 12 h after irradiation, and HO-1 expression peaked at 48 h after irradiation. RTP administration reduced radiation-induced intestinal damage, upregulated Nrf2/HO-1, improved physiological indexes, significantly decreased apoptosis and inflammatory factors, and upregulated HO-1, particularly at 48 h after irradiation. In conclusion, Nrf2 is activated in the early stage of radiation-induced intestinal injury and plays a protective role. RTP significantly ameliorates radiation-induced intestinal injury via the regulation of Nrf2 and its downstream protein HO-1.
Lipofibromatous Hamartoma in a Hispanic Patient with macrodactyly: a rare peripheral nerve tumor causing recurrent carpal tunnel syndrome
Authors: Pinci Marcantonio V , Olivella Gerardo , Rodríguez Manuel , Hess-Arcelay Hans W , Ramírez Norman , Foy-Parilla Christian 
Published: September 2020

AbstractThis is a rare case of a 42-year-old Hispanic male with macrodactyly who presented with recurrent carpal tunnel syndrome (CTS) due to Lipofibromatous Hamartoma (LFH). A slowly growing mass in his right palm with pain and numbness, along with motor and sensory deficits in the median nerve distribution, was reported since the past year prior to visit. Previous open carpal tunnel release was performed at 16 years of age due to CTS without complications. The diagnosis of LFH was made based on ultrasound, electromyography test and physical examination. An extended open carpal tunnel release with transection of transverse ligament was performed. The 24-month postoperative evaluation showed complete resolution of symptoms. This report illustrates the management of a recurrent CTS due to LFH in a Hispanic adult patient with macrodactyly that was successfully treated with a carpal tunnel decompression.
Activation of ACS7 in Arabidopsis affects vascular development and demonstrates a link between ethylene synthesis and cambial activity
Authors: Yang Shuo , Wang Sining , Li Shujia , Du Qian , Qi Liying , Wang Wenguang , Chen Jingtang , Wang Huanzhong 
Published: September 2020

Abstract
Ethylene is a gaseous hormone that affects many processes of plant growth and development. During vascular development, ethylene positively regulates cambial cell division in parallel with tracheary element differentiation inhibitory factor (TDIF) peptide signaling. In this study, we identified an ethylene overproducing mutant, acs7-d, exhibiting enhanced cambial activity and reduced wall development in fiber cells. Using genetic analysis, we found that ethylene signaling is necessary for the phenotypes of enhanced cambial cell division as well as defects in stem elongation and fiber cell wall development. Further, the cambial cell proliferation phenotype of acs7-d depends on WOX4, indicating that the two parallel pathways, ethylene and TDIF signaling, converge at WOX4 in regulating cambium activity. Gene expression analysis showed that ethylene impedes fiber cell wall biosynthesis through a conserved hierarchical transcriptional regulation. These results advance our understanding of the molecular mechanisms of ethylene in regulating vascular meristem activity.
PSB33 protein sustains photosystem II in plant chloroplasts under UV-A light
Authors: Nilsson Anders K , Pěnčík Aleš , Johansson Oskar N , Bånkestad Daniel , Fristedt Rikard , Suorsa Marjaana , Trotta Andrea , Novák Ondřej , Mamedov Fikret , Aro Eva-Mari , Burmeister Björn Lundin 
Published: September 2020

AbstractPlants can quickly and dynamically respond to spectral and intensity variations of the incident light. These responses include activation of developmental processes, morphological changes, and photosynthetic acclimation that ensure optimal energy conversion and minimal photoinhibition. Plant adaptation and acclimation to environmental changes have been extensively studied, but many details surrounding these processes remain elusive. The photosystem II (PSII)-associated protein PSB33 plays a fundamental role in sustaining PSII as well as in the regulation of the light antenna in fluctuating light. We investigated how PSB33 knock-out Arabidopsis plants perform under different light qualities. psb33 plants displayed a reduction of 88% of total fresh weight compared to wild type plants when cultivated at the boundary of UV-A and blue light. The sensitivity towards UV-A light was associated with a lower abundance of PSII proteins, which reduces psb33 plants’ capacity for photosynthesis. The UV-A phenotype was found to be linked to altered phytohormone status and changed thylakoid ultrastructure. Our results collectively show that PSB33 is involved in a UV-A light-mediated mechanism to maintain a functional PSII pool in the chloroplast.
A High-Level Overview of the Regulations Surrounding a Clinical Laboratory and Upcoming Regulatory Challenges for Laboratory Developed Tests
Authors: Graden Kevin C , Bennett Shannon A , Delaney Sarah R , Gill Hillary E , Willrich Maria A V 
Published: September 2020

Abstract

Objective
Regulations for clinical laboratories in the United States are complex. The goal of this review is to improve the clarity of laboratory-developed test (LDT) regulation to facilitate innovation.

Methods
A literature and regulation review of current legislation for compliance by U.S. clinical laboratories was performed, and examples of the steps to implement LDTs within compliance with the regulatory environment are shared.

Results
Many federal and state jurisdictions are critical to the functionality of a laboratory in addition to upcoming potential promulgation of the Verifying Accurate Leading-Edge IVCT Development Act. Increased regulation, although imperative to maintain consistent, high-standard clinical care, could mean additional costs for developers and healthcare while also hindering innovation.

Conclusion
An extensive discussion of proposed regulations for LDTs needs to occur. Laboratory testing requires the sustained use of innovative methods at a cost that will permit continued, timely, uninterrupted high-quality service.
Photometric modelling and VIS-IR albedo maps of Rhea from Cassini-VIMS
Authors: Filacchione G , Ciarniello M , D’Aversa E , Capaccioni F , Cerroni P , Buratti B J , Clark R N , Stephan K , Plainaki C 
Published: September 2020

ABSTRACT
Photometric correction based on the Shkuratov method is applied to derive visible and infrared albedo maps of Rhea from disc-resolved Cassini VIMS data. Differently from I/F images, albedo maps offer an optimal disentanglement of composition and physical properties of the surface from illumination-viewing effects and to study spectral variations occurring at hemispherical and local scales. A similar methodology has been already applied to Dione’s and Tethys’s data sets returned by VIMS. Following the same scheme also for Rhea, spectral albedo is derived at 59 wavelengths between 0.35 and 5.047 µm. Equigonal albedo maps are rendered in cylindrical projection with a 0.5$^\circ \, \times$ 0.5° angular resolution in latitude and longitude, corresponding to a maximum spatial resolution of 6.7 km bin−1. Apart from albedo, 0.35–0.55 and 0.55–0.95 µm spectral slopes and the water ice 1.5–2.0 µm band depth maps are computed from photometric-corrected data with the specific scope to investigate the leading-trailing hemisphere colour-albedo dichotomy and to constrain spectral properties above different morphological units including fresh craters (Inktomi) and bright tectonics features (Wakonda-Avaiki Chasmata).
EIF4A3-induced circular RNA ASAP1 promotes tumorigenesis and temozolomide resistance of glioblastoma via NRAS/MEK1/ERK1–2 signaling
Authors: Wei Yutian , Lu Chenfei , Zhou Peng , Zhao Lin , Lyu Xiao , Yin Jianxing , Shi ZhuMei , You Yongping 
Published: September 2020

Abstract

Background
Acquired chemoresistance is a major challenge in the clinical treatment of glioblastoma (GBM). Circular RNAs have been verified to play a role in tumor chemoresistance. However, the underlying mechanisms remain unclear. The aim of this study was to elucidate the potential role and molecular mechanism of circular (circ)RNA ADP-ribosylation factor GTPase activating proteins with Src homology 3 domain, ankyrin repeat and Pleckstrin homology domain 1 (circASAP1) in temozolomide (TMZ) resistance of GBM.

Methods
We analyzed circRNA alterations in recurrent GBM tissues relative to primary GBM through RNA sequencing. Real-time quantitative reverse transcription PCR verified the expression of circASAP1 in tissues and cells. Knockdown and overexpressed plasmids were used to evaluate the effect of circASAP1 on GBM cell proliferation and TMZ-induced apoptosis. Mechanistically, fluorescent in situ hybridization, dual-luciferase reporter, and RNA immunoprecipitation assays were performed to confirm the regulatory network of circASAP1/miR-502-5p/neuroblastoma Ras (NRAS). An intracranial tumor model was used to verify our findings in vivo.

Results
CircASAP1 expression was significantly upregulated in recurrent GBM tissues and TMZ-resistant cell lines. CircASAP1 overexpression enhanced GBM cell proliferation and TMZ resistance, which could be reduced by circASAP1 knockdown. Further experiments revealed that circASAP1 increased the expression of NRAS via sponging miR-502-5p. Moreover, circASAP1 depletion effectively restored the sensitivity of TMZ-resistant xenografts to TMZ treatment in vivo.

Conclusions
Our data demonstrate that circASAP1 exerts regulatory functions in GBM and that competing endogenous (ce)RNA-mediated microRNA sequestration might be a potential therapeutic strategy for GBM treatment.
Integral field unit for the existing imaging and spectroscopy instrument, FOCAS
Authors: Ozaki Shinobu , Fukushima Mitsuhiro , Iwashita Hikaru , Mitsui Kenji , Hattori Takashi , Lee Chien-Hsiu , Tanaka Yoko , Tsuzuki Toshihiro , Miyazaki Satoshi , Yamashita Takuya , Okada Norio , Obuchi Yoshiyuki 
Published: September 2020

Abstract
The Faint Object Camera and Spectrograph (FOCAS) is an optical imaging and spectroscopy instrument for the Subaru Telescope. It has been a workhorse instrument since the first-light phase of the telescope. We describe an integral field unit (IFU) that has recently been installed in FOCAS. The IFU utilizes an image slicer that divides a ${13{^{\prime \prime }_{.}}5}$ × ${10{^{\prime \prime }_{.}}0}$ field of view into 23 stripes, with a width of ${0{^{\prime \prime }_{.}}435}$. A sky spectrum separated from an object by approximately ${5{^{\prime }_{.}}2}$ can be obtained at the same time as an object spectrum. Test observations confirmed that the image quality of the IFU does not degrade the ${0{^{\prime \prime }_{.}}435}$ sampling, and that slice width and length are consistent with the design. Highly reflective multilayer dielectric coatings were coated on all the mirrors in the IFU, thereby offering a high mean IFU throughput of ∼85% over the field. However, the outer part of the field showed throughput degradation, which was mainly caused by vignetting as a result of misalignment. The flat-fielding accuracy was degraded by the vignetting, with the variation depending on the direction of the telescope.
Coeliac disease is a risk factor for the development of seronegative arthritis in patients with autoimmune thyroid disease
Authors: Lupoli Gelsy Arianna , Tasso Marco , Costa Luisa , Caso Francesco , Scarpa Raffaele , Del Puente Antonio , Peluso Rosario 
Published: September 2020

AbstractObjectiveHashimoto’s thyroiditis is known to cluster with other systemic autoimmune disorders. Rheumatic manifestations, such as a seronegative non-erosive polyarthritis have been described. The aim of this study was to evaluate the characteristics and the prevalence of rheumatic features in thyroiditis patients, and to ascertain whether the association with systemic autoimmune disorders improved the arthritis manifestations.MethodsIn total, 180 thyroiditis patients were enrolled. Major clinical and demographic characteristics have been recorded. Patients underwent a rheumatological clinical assessment and extra-articular manifestations allowing for a differential diagnosis with systemic autoimmune diseases and spondyloarthropathy. Presence of systemic autoimmune diseases was recorded.ResultsA total of 8.33% of thyroiditis patients shown a peripheral inflammatory arthritis (P = 0.002). Female gender (P = 0.042) and thyroid peroxidase (TPOAbs) positivity (P = 0.001) were more frequent. In total, 37 patients had systemic autoimmune diseases (P = 0.0003). A significant high prevalence of coeliac disease and Addison disease was found (P = 0.034 and P = 0.049, respectively). In patients with coeliac disease, the articular manifestations were more frequent (21.21%) (P = 0.001) and the risk to develop joint involvement was 2.96.ConclusionAlthough we found an articular involvement in about one-third of thyroiditis patients, the prevalence of inflammatory arthropathy was only 8.33%. The prevalence of other coexisting autoimmune disorders was 34.26% with a significant prevalence of coeliac disease (7.41%). Thyroiditis patients with coeliac disease have an articular involvement more frequently than those without. In these patients, we have found a high risk of developing arthritis than patients with only thyroiditis, suggesting cumulative autoimmune effects in the developing articular involvement.
Ageing unequally in Europe
Authors: Olivera Javier 
Published: September 2020

AbstractTo shed light on the quality of the ageing experience in Europe and its heterogeneity, this study adapts and computes the Active Ageing Index—an index constructed at the country level to monitor ageing quality in Europe—at the individual level. This strategy allows the measuring of inequality in the experience of active ageing and is flexible enough to consider different value judgements in the overall assessment of the quality of life while ageing. The study examines the predictors of this inequality by using regressions with a Gini-recentred influence function. It finds that education plays a very significant role in reducing inequality, though its influence varies across countries. Furthermore, the study uncovers large variance in the quality of the ageing experience across Europe. For instance, more than 50% of the populations of Romania, Lithuania and Bulgaria show a level of active ageing quality lower than that of the bottom decile of the distribution in Sweden.
The Limits of Gaining Rights while Remaining Marginalized: The Deferred Action for Childhood Arrivals (DACA) Program and the Psychological Wellbeing of Latina/o Undocumented Youth
Authors: Patler Caitlin , Hamilton Erin R , Savinar Robin L 
Published: September 2020

Abstract
Policies that expand the rights of marginalized groups provide an additional level of structural integration, but these changes do not always come with broad social acceptance or recognition. What happens when a legally marginalized group attains increased rights but not full political or social inclusion? In particular, what are the mental health implications of these transitions for impacted groups? We bring together theories of liminal legality and stress process to offer a framework for understanding how expansions in the legal rights of a highly politicized and vulnerable social group can be initially beneficial, but can attenuate due to renewed or new stress events, chronic stressors, and anticipatory stressors. We use the case of Latina/o immigrant youth who transitioned from undocumented legal status to temporarily protected status under the Deferred Action for Childhood Arrivals (DACA) program. Analyses of representative California statewide survey data from 2007 to 2018, combined with surveys and in-depth interviews with DACA recipients, suggest that without full social and structural inclusion, legal transitions that expand rights will produce short-term psychological benefits that do not hold up over time.
Sleep deprivation and compensatory cognitive effort on a visual information processing task
Authors: Sullan Molly J , Drummond Sean P A , Granholm Eric 
Published: September 2020

AbstractStudy ObjectivesTotal sleep deprivation (TSD) is often associated with worse performance on tasks of attention and working memory, but some studies show no performance changes. One possibility is that greater compensatory cognitive effort is put forth to achieve similar results after TSD. We aimed to better understand the relationship between TSD, cognitive engagement, and performance outcomes following TSD.MethodsTwenty healthy adults completed cognitive testing following a night of normal sleep and again after ~55 hours of TSD. Participants detected target letters in low (3-item) and high (10-item) load visual letter displays on the span of apprehension task with concurrent pupillometry, a measure of cognitive effort.ResultsWe found significantly poorer detection accuracy and marginally longer response times following TSD across both arrays. In both arrays, significantly greater preparatory pupillary responses were found just prior to array onset. There was also a significant session by array interaction for pupillary responses, such that significantly greater dilation was found for the 3-letter array after TSD, while a nonsignificant decline in dilation was found following the 10-letter array after TSD.ConclusionsThese results suggest a complex relationship between attentional control and cognitive resource allocation following TSD. Sleep-deprived individuals may allocate more compensatory cognitive effort to easier tasks but choose to disengage from more challenging cognitive tasks that have little perceived reward or probability of success to preserve diminishing cognitive resources. More work is needed to better delineate the underlying neurological systems involved in these processing load-dependent attentional control mechanisms after TSD.
The effects of enzymatically treated soybean meal on growth performance and intestinal structure, barrier integrity, inflammation, oxidative status, and volatile fatty acid production of nursery pigs
Authors: Ruckman Leigh A , Petry Amy L , Gould Stacie A , Kerr Brian J , Patience John F 
Published: September 2020

Abstract
The objective of this experiment was to determine the impact of diets containing increasing amounts of enzymatically treated soybean meal (ESBM) but decreasing amounts of soybean meal (SBM) on growth performance, intestinal structure, and barrier integrity, inflammation, and oxidative status in weaned pigs. A total of 480 pigs [6.3 ± 1.2 kg body weight (BW)] were blocked by initial BW and pens (n = 12 per treatment) were randomly allotted to one of four dietary treatments. Diets were fed in three phases (days 0–14, 14–28, and 28–35) over a 35-d period. The four dietary treatments consisted of a negative control diet (NC): the NC with 7.0% ESBM (ESBM1), the NC with 14.0% ESBM (ESBM2), and the NC with 21.0% ESBM (ESBM3). Soybean meal was reduced proportionately in each treatment. In phase 2, ESBM inclusion was decreased by 50% (3.5%, 7.0%, and 10.5% ESBM, respectively); phase 3 was a common diet and contained no ESBM. Fecal score was visually ranked weekly using a four-point scale. Intestinal tissue, digesta, and blood samples were collected from 48 pigs (1 per pen) on day 10. Data were analyzed using PROC MIXED of SAS (9.4) with pen as the experimental unit; diet and block were considered fixed effects. Linear and quadratic contrasts were used to determine the effect of increasing ESBM. Overall, ESBM2 and ESBM3 decreased final BW, average daily gain, and average daily feed intake compared to NC and ESBM1 (diet, P &lt; 0.05; linear, P &lt; 0.05). Overall fecal score (diet, P &lt; 0.05) and fecal dry matter (P &lt; 0.05) were improved by feeding ESBM diets compared to NC. Volatile fatty acid (VFA) concentration of acetate, propionate, butyrate, and total VFA in ileal contents increased as ESBM inclusion increased (P &lt; 0.05). Colonic VFA concentration was not impacted (P &gt; 0.10). Total antioxidant capacity was increased by ESBM (P &lt; 0.05). The concentration of mucosal interleukin-4 increased as the inclusion of ESBM increased (linear, P &lt; 0.05). Messenger ribonucleic acid abundance of occludin and zonula-occludens-1 in ileal tissue was increased by ESBM1 or ESBM2 (P &lt; 0.05). In conclusion, increasing the dietary levels of ESBM over 7% had a negative impact on nursery pig performance, but ESBM positively impacted fecal score. Feeding ESBM improved oxidative status and intestinal barrier integrity while increasing ileal VFA production but had minimal impact on intestinal inflammation or morphology. Further research is needed to determine the optimal inclusion level of ESBM.
Canopy position affects photosynthesis and anatomy in matureEucalyptustrees in elevated CO2
Authors: Crous K Y , Campany C , López R , Cano F J , Ellsworth D S 
Published: September 2020

AbstractLeaves are exposed to different light conditions according to their canopy position, resulting in structural and anatomical differences with consequences for carbon uptake. While these structure–function relationships have been thoroughly explored in dense forest canopies, such gradients may be diminished in open canopies, and they are often ignored in ecosystem models. We tested within-canopy differences in photosynthetic properties and structural traits in leaves in a mature Eucalyptus tereticornis canopy exposed to long-term elevated CO2 for up to 3 years. We explored these traits in relation to anatomical variation and diffusive processes for CO2 (i.e., stomatal conductance, gs, and mesophyll conductance, gm) in both upper and lower portions of the canopy receiving ambient and elevated CO2. While shade resulted in 13% lower leaf mass per area ratio (MA) in lower versus upper canopy leaves, there was no relationship between leaf nitrogen concentration (Nmass) and canopy gap fraction. Both maximum carboxylation capacity (Vcmax) and maximum electron transport (Jmax) were ~18% lower in shaded leaves and were also reduced by ~22% with leaf aging. In mature leaves, we found no canopy differences for gm or gs, despite anatomical differences in MA, leaf thickness and mean mesophyll thickness between canopy positions. There was a positive relationship between net photosynthesis and gm or gs in mature leaves. Mesophyll conductance was negatively correlated with mean parenchyma length, suggesting that long palisade cells may contribute to a longer CO2 diffusional pathway and more resistance to CO2 transfer to chloroplasts. Few other relationships between gm and anatomical variables were found in mature leaves, which may be due to the open crown of Eucalyptus. Consideration of shade effects and leaf-age-dependent responses to photosynthetic capacity and mesophyll conductance are critical to improve canopy photosynthesis models and will improve the understanding of long-term responses to elevated CO2 in tree canopies.