bluuebunny/crossref_metadata_2025_split huggingface dataset:
An ethnographic study exploring factors that minimize lunch waste in Tokyo elementary schools
Authors: Izumi Betty T , Akamatsu Rie , Byker Shanks Carmen , Fujisaki Kahori 
Published: January 2020

AbstractObjective:To explore factors that minimize lunch waste in Tokyo elementary schools and to consider how such factors can be modified and applied in US schools.Design:Focused ethnographic study using interviews, observation, participant observation and document review. Data were analysed using thematic analysis.Setting:Tokyo, Japan.Participants:Five school dietitians participated in the study. Data collection methods included in-depth interviews, observation of nutrition education lessons, participant observation of school lunchtime and review of relevant school documents (e.g. lunch menus, food waste records).Results:Five themes emerged from the analysis: (i) reinforcement of social norms to eat without waste; (ii) menu planning to increase exposure to unfamiliar and/or disliked foods; (iii) integration of food and nutrition education into the school curriculum; (iv) teacher lunchtime practices related to portion sizes, distributing leftover food and time management; and (v) engagement of students in reducing school lunch waste. Practical and tangible applications to US schools include measuring and reporting lunch waste to influence social norms, teaching students about the importance of reducing food waste, offering flexible school lunch portion sizes and providing students with meaningful opportunities to contribute to solving the problem of school lunch waste.Conclusions:Japan offers a model for minimizing school lunch waste through a holistic approach that includes factors that operate at and interact across multiple levels of society. Modifying and applying such an approach in US schools is worth considering given the urgent need to address food waste in order to support healthy diets and sustainable food systems.
Landscape positions dictating crop fertilizer responses in wheat-based farming systems of East African Highlands
Authors: Amede Tilahun , Gashaw Tadesse , Legesse Gizachew , Tamene Lulseged , Mekonen Kindu , Thorne Peter , Schultz Steffen 
Published: January 2020

AbstractImproving fertilizer use efficiency has remained a challenge, particularly for small-scale farming in undulating ‘abnormal’ landscapes of East Africa. Milne's 1930s concept on ‘Catena’ was considered as a breakthrough in understanding soil variability and its implication on productivity in East African highlands. However, there is limited information on how the ‘Catena’ features could be used for fine tuning fertilizer recommendations. We initiated multiple on-farm replicated experiments in three wheat-growing districts (Endamohoni, Lemo and Worreilu) in the Ethiopian highlands in 2014, 2015 and 2016 to assess landscape positions affecting crop-nutrient responses, identify yield limiting nutrients across the ‘Catena’ (N, P, K, S and Zn) and quantify effects of landscape positions on resources use efficiency. We clustered farmlands across the ‘Catena’ (Hillslopes, Midslopes and Footslopes) based on land scape positions in the respective locations. Wheat yield was more strongly and significantly affected by landscape positions (P < 0.001) than by nutrient sources or rates. The crop response to fertilizers was 50 to 300% higher in foot slopes than in hillslopes, depending on locations and inputs levels. With increasing slope, there was a decrease in a crop fertilizer response due to a significant decrease in soil organic carbon, clay content and soil water content, with r2 of 0.95, 0.86 and 0.96, respectively. The difference in the crop response between landscape positions was significantly higher (P < 0.05) with higher rates of nutrient applications (>N92 P46) while differences between landscape positions diminish at lower rates. Yield benefits due to application of K was significant only in the dry years (P < 0.05), while there was hardly any yield benefit from the application of zinc and sulfur. The crop nitrogen recovery fraction and crop water productivity decreased with an increasing slope regardless of nutrient combinations. The results indicated that the landscape position could be considered as a proxy indicator for targeted fertilizer application, particularly in farms with undulating topographic features. Hillslopes are better served by the application of organic fertilizers along with conservation measures as applying higher rates of mineral fertilizer in hillslopes would rather increase the risk of downstream nutrient movement.
Plume‐Induced Subduction Initiation: Single‐Slab or Multi‐Slab Subduction?
Authors: Baes Marzieh , Sobolev Stephan , Gerya Taras , Brune Sascha 
Published: January 2020

AbstractInitiation of subduction following the impingement of a hot buoyant mantle plume is one of the few scenarios that allow breaking the lithosphere and recycling a stagnant lid without requiring any preexisting weak zones. Here, we investigate factors controlling the number and shape of retreating subducting slabs formed by plume‐lithosphere interaction. Using 3‐D thermomechanical models we show that the deformation regime, which defines formation of single‐slab or multi‐slab subduction, depends on several parameters such as age of oceanic lithosphere, thickness of the crust and large‐scale lithospheric extension rate. Our model results indicate that on present‐day Earth multi‐slab plume‐induced subduction is initiated only if the oceanic lithosphere is relatively young (<30–40 Myr, but >10 Myr), and the crust has a typical thickness of 8 km. In turn, development of single‐slab subduction is facilitated by older lithosphere and pre‐imposed extensional stresses. In early Earth, plume‐lithosphere interaction could have led to formation of either episodic short‐lived circular subduction when the oceanic lithosphere was young or to multi‐slab subduction when the lithosphere was old.
The Relationship Between Magmatism and Deformation Along the Intra‐arc Strike‐Slip Atacama Fault System, Northern Chile
Authors: Seymour N. M. , Singleton J. S. , Mavor S. P. , Gomila R. , Stockli D. F. , Heuser G. , Arancibia G. 
Published: January 2020

AbstractMagmatic arcs may play a major role in the initiation, behavior, and abandonment of intra‐arc strike‐slip systems. Here we present zircon U‐Pb and (U‐Th)/He geochronology/thermochronology with new mapping to relate Coastal Cordillera arc magmatism to sinistral shear along the Atacama fault system (AFS) in northern Chile. New dates from 18 intrusions along the AFS between 24.6°S and 27°S compiled with published data record a minor Early Jurassic magmatic pulse (195–175 Ma), broad latest Jurassic to Early Cretaceous (150–120 Ma) pulse, and a minor younger (120–105 Ma) pulse. Mylonitization occurred only along the margins of Early Cretaceous plutons and surrounding Paleozoic metasedimentary rock, whereas Jurassic plutons and metasedimentary rocks away from Early Cretaceous plutons lack mylonitic fabrics. Early Cretaceous magmatism facilitated AFS deformation by thermally weakening the crust with elevated geothermal gradients that enabled mylonitization to take place at ~5‐ to 7‐km depths and low stresses. Spatial variability of pluton emplacement produced significant rheological heterogeneity, giving rise to a highly segmented fault system that did not originate as a regional‐scale shear zone. Synkinematic dikes (~120–117 Ma) cut mylonitic fabrics, and a postkinematic dike (~110 Ma) records the end stages of slip. The cessation of slip coincided with cooling below ~180 °C at ~116–99 Ma as arc magmatism migrated eastward and geothermal gradients relaxed, coeval with a major reorganization in plate motion and the onset of seafloor spreading in the south Atlantic.
Digital Rock Segmentation for Petrophysical Analysis With Reduced User Bias Using Convolutional Neural Networks
Authors: Niu Yufu , Mostaghimi Peyman , Shabaninejad Mehdi , Swietojanski Pawel , Armstrong Ryan T. 
Published: January 2020

AbstractPore‐scale digital images are usually obtained from microcomputed tomography data that has been segmented into void and grain space. Image segmentation is a crucial step in the process of digital rock analysis that can influence pore‐scale characterization studies and/or the numerical simulation of petrophysical properties. This is concerning since all segmentation methods have user‐selected parameters that result in biases. Convolutional neural networks (CNNs) provide a way forward since once trained, CNN can provide consistent and reliable image segmentation with no user‐defined inputs. In this paper, a CNN is used to segment digital sandstone data, and various ground truth data sets are tested. The ground truth images are created based on high‐resolution microcomputed tomography data and corresponding scanning electron microscope data. The results are evaluated in terms of porosity, permeability, and pore size distribution computed from the segmented data. We find that watershed‐based segmentation provides a wide range of possible petrophysical values depending on user‐selected thresholds, whereas CNN provides a smaller variance when trained on scanning electron microscope data. It can be concluded that CNN offers a reliable and consistent way to segment digital sandstone data for petrophysical analyses.
Daratumumab plus lenalidomide and dexamethasone in relapsed/refractory multiple myeloma: extended follow-up of POLLUX, a randomized, open-label, phase 3 study
Authors: Bahlis Nizar J. , Dimopoulos Meletios A. , White Darrell J. , Benboubker Lotfi , Cook Gordon , Leiba Merav , Ho P. Joy , Kim Kihyun , Takezako Naoki , Moreau Philippe , Kaufman Jonathan L. , Krevvata Maria , Chiu Christopher , Qin Xiang , Okonkwo Linda , Trivedi Sonali , Ukropec Jon , Qi Ming , San-Miguel Jesus 
Published: January 2020

AbstractIn POLLUX, daratumumab (D) plus lenalidomide/dexamethasone (Rd) reduced the risk of disease progression or death by 63% and increased the overall response rate (ORR) versus Rd in relapsed/refractory multiple myeloma (RRMM). Updated efficacy and safety after >3 years of follow-up are presented. Patients (N = 569) with ≥1 prior line received Rd (lenalidomide, 25 mg, on Days 1–21 of each 28-day cycle; dexamethasone, 40 mg, weekly) ± daratumumab at the approved dosing schedule. Minimal residual disease (MRD) was assessed by next-generation sequencing. After 44.3 months median follow-up, D-Rd prolonged progression-free survival (PFS) in the intent-to-treat population (median 44.5 vs 17.5 months; HR, 0.44; 95% CI, 0.35–0.55; P < 0.0001) and in patient subgroups. D-Rd demonstrated higher ORR (92.9 vs 76.4%; P < 0.0001) and deeper responses, including complete response or better (56.6 vs 23.2%; P < 0.0001) and MRD negativity (10–5; 30.4 vs 5.3%; P < 0.0001). Median time to next therapy was prolonged with D-Rd (50.6 vs 23.1 months; HR, 0.39; 95% CI, 0.31–0.50; P < 0.0001). Median PFS on subsequent line of therapy (PFS2) was not reached with D-Rd versus 31.7 months with Rd (HR, 0.53; 95% CI, 0.42–0.68; P < 0.0001). No new safety concerns were reported. These data support using D-Rd in patients with RRMM after first relapse.
Protein tyrosine phosphatase 4A3 (PTP4A3/PRL-3) drives migration and progression of T-cell acute lymphoblastic leukemia in vitro and in vivo
Authors: Wei M. , Haney M. G. , Rivas D. R. , Blackburn J. S. 
Published: January 2020

AbstractT-cell acute lymphoblastic leukemia (T-ALL) is an aggressive blood cancer. There are no immunotherapies and few molecularly targeted therapeutics available for treatment of this malignancy. The identification and characterization of genes and pathways that drive T-ALL progression are critical for the development of new therapies for T-ALL. Here, we determined that the protein tyrosine phosphatase 4A3 (PTP4A3 or PRL-3) plays a critical role in T-ALL initiation and progression by promoting leukemia cell migration. PRL-3 is highly expressed in patient T-ALL samples at both the mRNA and protein levels compared to normal lymphocytes. Knock-down of PRL-3 expression using short-hairpin RNA (shRNA) in human T-ALL cell lines significantly impeded T-ALL cell migration capacity in vitro and reduced their ability to engraft and proliferate in vivo in xenograft mouse models. Additionally, PRL-3 overexpression in aMyc-induced zebrafish T-ALL model significantly accelerated disease onset and shortened the time needed for cells to enter blood circulation. Reverse-phase protein array (RPPA) and gene set enrichment analysis (GSEA) revealed that the SRC signaling pathway is affected by PRL-3. Immunoblot analyses validated that manipulation of PRL-3 expression in T-ALL cells affected the SRC signaling pathway, which is directly involved in cell migration, although Src was not a direct substrate of PRL-3. More importantly, T-ALL cell growth and migration were inhibited by small molecule inhibition of PRL-3, suggesting that PRL-3 has potential as a therapeutic target in T-ALL. Taken together, our study identifies PRL-3 as an oncogenic driver in T-ALL both in vitro and in vivo and provides a strong rationale for targeted therapies that interfere with PRL-3 function.
Two faces of bivalent domain regulate VEGFA responsiveness and angiogenesis
Authors: Chen Jiahuan , Liang Xiaodong , Zhang Shasha , Wang Shiyan , Garcia Sara P. , Yan Pengyi , Yu Huijing , Li Zixuan , Liu Li , Zhang Fang , Wei Weiting , Le Huangying , Zhang Yan , Yuan Guo-cheng , Chen Sun , Chen Yingwei , Sun Kun , Pu William T. , Zhang Bing 
Published: January 2020

AbstractThe bivalent domain (BD) at promoter region is an unique epigenetic feature poised for activation or repression during cell differentiation in embryonic stem cell. However, the function of BDs in already differentiated cells remains exclusive. By profiling the epigenetic landscape of endothelial cells during VEGFA (vascular endothelial growth factor A) stimulation, we discovered that BDs are widespread in endothelial cells and preferentially marked genes responsive to VEGFA. The BDs responsive to VEGFA have more permissive chromatin environment comparing to other BDs. The initial activation of bivalent genes depends on RNAPII pausing release induced by EZH1 rather than removal of H3K27me3. The later suppression of bivalent gene expression depended on KDM5A recruitment by its interaction with PRC2. Importantly, EZH1 promoted both in vitro and in vivo angiogenesis by upregulating EGR3, whereas KDM5A dampened angiogenesis. Collectively, this study demonstrates a novel dual function of BDs in endothelial cells to control VEGF responsiveness and angiogenesis.
Inhibition of AIM2 inflammasome activation alleviates GSDMD-induced pyroptosis in early brain injury after subarachnoid haemorrhage
Authors: Yuan Bin , Zhou Xiao-ming , You Zong-qi , Xu Wei-dong , Fan Jie-mei , Chen Shu-juan , Han Yan-ling , Wu Qi , Zhang Xin 
Published: January 2020

AbstractOnly a few types of inflammasomes have been described in central nervous system cells. Among these, the absent in melanoma 2 (AIM2) inflammasome is primarily found in neurons, is highly specific and can be activated only by double-stranded DNA. Although it has been demonstrated that the AIM2 inflammasome is activated by poly(deoxyadenylic-deoxythymidylic) acid sodium salt and leads to pyroptotic neuronal cell death, the role of AIM2 inflammasome-mediated pyroptosis in early brain injury (EBI) after subarachnoid haemorrhage (SAH) has rarely been studied. Thus, we designed this study to explore the mechanism of gasdermin D(GSDMD)-induced pyroptosis mediated by the AIM2 inflammasome in EBI after SAH. The level of AIM2 from the cerebrospinal fluid (CSF) of patients with SAH was detected. The pathway of AIM2 inflammasome-mediated pyroptosis, the AIM2/Caspase-1/GSDMD pathway, was explored after experimental SAH in vivo and in primary cortical neurons stimulated by oxyhaemoglobin (oxyHb) in vitro. Then, we evaluated GSDMD-induced pyroptosis mediated by the AIM2 inflammasome in AIM2 and caspase-1- deficient mice and primary cortical neurons generated through lentivirus (LV) knockdown. Compared with that of the control samples, the AIM2 level in the CSF of the patients with SAH was significantly increased. Pyroptosis-associated proteins mediated by the AIM2 inflammasome were significantly increased in vivo and in vitro following experimentally induced SAH. After AIM2 and caspase-1 were knocked down by an LV, GSDMD-induced pyroptosis mediated by the AIM2 inflammasome was alleviated in EBI after SAH. Intriguingly, when caspase-1 was knocked down, apoptosis was significantly suppressed via impeding the activation of caspase-3. GSDMD-induced pyroptosis mediated by the AIM2 inflammasome may be involved in EBI following SAH. The inhibition of AIM2 inflammasome activation caused by knocking down AIM2 and caspase-1 alleviates GSDMD-induced pyroptosis in EBI after SAH.
Laminated self-standing covalent organic framework membrane with uniformly distributed subnanopores for ionic and molecular sieving
Authors: Li Yang , Wu Qianxun , Guo Xinghua , Zhang Meicheng , Chen Bin , Wei Guanyi , Li Xing , Li Xiaofeng , Li Shoujian , Ma Lijian 
Published: January 2020

AbstractThe preparation of subnanoporous covalent-organic-framework (COF) membranes with high performance for ion/molecule sieving still remains a great challenge. In addition to the difficulties in fabricating large-area COF membranes, the main reason is that the pore size of 2D COFs is much larger than that of most gas molecules and/or ions. It is urgently required to further narrow their pore sizes to meet different separation demands. Herein, we report a simple and scalable way to grow large-area, pliable, free-standing COF membranes via a one-step route at organic–organic interface. The pore sizes of the membranes can be adjusted from >1 nm to sub-nm scale by changing the stacking mode of COF layers from AA to AB stacking. The obtained AB stacking COF membrane composed of highly-ordered nanoflakes is demonstrated to have narrow aperture (∼0.6 nm), uniform pore distribution and shows good potential in organic solvent nanofiltration, water treatment and gas separation.
Macrophages directly contribute collagen to scar formation during zebrafish heart regeneration and mouse heart repair
Authors: Simões Filipa C. , Cahill Thomas J. , Kenyon Amy , Gavriouchkina Daria , Vieira Joaquim M. , Sun Xin , Pezzolla Daniela , Ravaud Christophe , Masmanian Eva , Weinberger Michael , Mayes Sarah , Lemieux Madeleine E. , Barnette Damien N. , Gunadasa-Rohling Mala , Williams Ruth M. , Greaves David R. , Trinh Le A. , Fraser Scott E. , Dallas Sarah L. , Choudhury Robin P. , Sauka-Spengler Tatjana , Riley Paul R. 
Published: January 2020

AbstractCanonical roles for macrophages in mediating the fibrotic response after a heart attack include extracellular matrix turnover and activation of cardiac fibroblasts to initiate collagen deposition. Here we reveal that macrophages directly contribute collagen to the forming post-injury scar. Unbiased transcriptomics shows an upregulation of collagens in both zebrafish and mouse macrophages following heart injury. Adoptive transfer of macrophages, from either collagen-tagged zebrafish or adult mouse GFPtpz-collagen donors, enhances scar formation via cell autonomous production of collagen. In zebrafish, the majority of tagged collagen localises proximal to the injury, within the overlying epicardial region, suggesting a possible distinction between macrophage-deposited collagen and that predominantly laid-down by myofibroblasts. Macrophage-specific targeting of col4a3bpa and cognate col4a1 in zebrafish significantly reduces scarring in cryoinjured hosts. Our findings contrast with the current model of scarring, whereby collagen deposition is exclusively attributed to myofibroblasts, and implicate macrophages as direct contributors to fibrosis during heart repair.
Pattern generation and symbolic dynamics in a nanocontact vortex oscillator
Authors: Yoo Myoung-Woo , Rontani Damien , Létang Jérémy , Petit-Watelot Sébastien , Devolder Thibaut , Sciamanna Marc , Bouzehouane Karim , Cros Vincent , Kim Joo-Von 
Published: January 2020

AbstractHarnessing chaos or intrinsic nonlinear behaviours of dynamical systems is a promising avenue toward unconventional information processing technologies. In this light, spintronic devices are promising because of the inherent nonlinearity of magnetization dynamics. Here, we demonstrate experimentally the potential for chaos-based schemes using nanocontact vortex oscillators by unveiling and characterizing their waveform patterns and symbolic dynamics using time-resolved electrical measurements. We dissociate nonlinear deterministic patterns from thermal fluctuations and show that the emergence of chaos results in the unpredictable alternation of well-defined patterns. With phase-space reconstruction techniques, we perform symbolic analyses of the time series and show that the oscillator exhibits maximal entropy and complexity at the centre of its incommensurate region. This suggests that such vortex-based systems are promising nanoscale sources of entropy that could be exploited for information processing.
Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia
Authors: Llorens Franc , Hermann Peter , Villar-Piqué Anna , Diaz-Lucena Daniela , Nägga Katarina , Hansson Oskar , Santana Isabel , Schmitz Matthias , Schmidt Christian , Varges Daniela , Goebel Stefan , Dumurgier Julien , Zetterberg Henrik , Blennow Kaj , Paquet Claire , Baldeiras Inês , Ferrer Isidro , Zerr Inga 
Published: January 2020

AbstractThe clinical diagnosis of vascular dementia (VaD) is based on imaging criteria, and specific biochemical markers are not available. Here, we investigated the potential of cerebrospinal fluid (CSF) lipocalin 2 (LCN2), a secreted glycoprotein that has been suggested as mediating neuronal damage in vascular brain injuries. The study included four independent cohorts with a total n = 472 samples. LCN2 was significantly elevated in VaD compared to controls, Alzheimer’s disease (AD), other neurodegenerative dementias, and cognitively unimpaired patients with cerebrovascular disease. LCN2 discriminated VaD from AD without coexisting VaD with high accuracy. The main findings were consistent over all cohorts. Neuropathology disclosed a high percentage of macrophages linked to subacute infarcts, reactive astrocytes, and damaged blood vessels in multi-infarct dementia when compared to AD. We conclude that CSF LCN2 is a promising candidate biochemical marker in the differential diagnosis of VaD and neurodegenerative dementias.
Reversible interconversion between methanol-diamine and diamide for hydrogen storage based on manganese catalyzed (de)hydrogenation
Authors: Shao Zhihui , Li Yang , Liu Chenguang , Ai Wenying , Luo Shu-Ping , Liu Qiang 
Published: January 2020

AbstractThe development of cost-effective, sustainable, and efficient catalysts for liquid organic hydrogen carrier systems is a significant goal. However, all the reported liquid organic hydrogen carrier systems relied on the use of precious metal catalysts. Herein, a liquid organic hydrogen carrier system based on non-noble metal catalysis was established. The Mn-catalyzed dehydrogenative coupling of methanol and N,N’-dimethylethylenediamine to form N,N’-(ethane-1,2-diyl)bis(N-methylformamide), and the reverse hydrogenation reaction constitute a hydrogen storage system with a theoretical hydrogen capacity of 5.3 wt%. A rechargeable hydrogen storage could be achieved by a subsequent hydrogenation of the resulting dehydrogenation mixture to regenerate the H2-rich compound. The maximum selectivity for the dehydrogenative amide formation was 97%.