bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sweet and magnetic: Succession and CAZyme expression of marine bacterial communities encountering a mix of alginate and pectin particles
Authors: Bunse Carina , Koch Hanna , Breider Sven , Simon Meinhard , Wietz Matthias 
Published: December 2020

ABSTRACTPolysaccharide particles are an important nutrient source and microhabitat for marine bacteria. However, substrate-specific bacterial dynamics in a mixture of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed. Here, we studied the composition, functional diversity and gene expression of marine bacterial communities encountering a mix of alginate and pectin particles. Communities were collected above macroalgal forests near Helgoland Island − where polysaccharide-rich particles might regularly occur − and exposed to a mix of magnetic particles of each polysaccharide, allowing the targeted evaluation by particle type. Amplicon, metagenome and metatranscriptome sequencing revealed that particle-associated (PA) and free-living (FL) communities significantly differed in composition and metabolism, whereas dynamics on alginate and pectin particles were unexpectedly similar. Amplicon sequence variants (ASVs) fromTenacibaculum,Colwellia,PsychrobiumandPsychromonasdominated the community on both particle types. Corresponding metagenome-assembled genomes (MAGs) expressed diverse alginate lyases, several co-localized in polysaccharide utilization loci. One low-abundance MAG related toCatenovulumshowed pectin specialization through upregulated GH53 and GH105 genes. A singleGlaciecolaASV dominated the FL fraction, likely persisting on particle-derived oligomers through different glycoside hydrolases. The bacterial preference for alginate, whereas pectin mainly served as colonization scaffold, illuminates substrate-driven microbial dynamics within mixed polysaccharide resources. Moreover, elevated ammonium metabolism signifies nitrogen availability as important factor on particles, whereas elevated methylcitrate and glyoxylate cycles illustrate nutrient-limited conditions in the surrounding water. These insights expand our understanding of bacterial microscale ecology, niche specialization and the biological carbon pump in macroalgae-rich habitats.
The context-dependent, combinatorial logic of BMP signaling
Authors: Klumpe Heidi , Langley Matthew A. , Linton James M. , Su Christina J. , Antebi Yaron E. , Elowitz Michael B. 
Published: December 2020

SummaryCell-cell communication systems typically comprise families of ligand and receptor variants that function together in combinations. Pathway activation depends in a complex way on which ligands are present and what receptors are expressed by the signal-receiving cell. To understand the combinatorial logic of such a system, we systematically measured pairwise Bone Morphogenetic Protein (BMP) ligand interactions in cells with varying receptor expression. Ligands could be classified into equivalence groups based on their profile of positive and negative synergies with other ligands. These groups varied with receptor expression, explaining how ligands can functionally replace each other in one context but not another. Context-dependent combinatorial interactions could be explained by a biochemical model based on competitive formation of alternative signaling complexes with distinct activities. Together, these results provide insights into the roles of BMP combinations in developmental and therapeutic contexts and establish a framework for analyzing other combinatorial, context-dependent signaling systems.
Reusability and composability in process description maps: RAS-RAF-MEK-ERK signalling
Authors: Mazein Alexander , Rougny Adrien , Karr Jonathan R. , Rodriguez Julio Saez , Ostaszewski Marek , Schneider Reinhard 
Published: December 2020

AbstractDetailed maps of the molecular basis of the disease are powerful tools for interpreting data and building predictive models. Modularity and composability are considered necessary network features for large-scale collaborative efforts to build comprehensive molecular descriptions of disease mechanisms. An effective way to create and manage large systems is to compose multiple subsystems. Composable network components could effectively harness the contributions of many individuals and enable teams to seamlessly assemble many individual components into comprehensive maps. We examine manually-built versions of the RAS-RAF-MEK-ERK cascade from the Atlas of Cancer Signalling Network, PANTHER and Reactome databases and review them in terms of their reusability and composability for assembling new disease models. We identify design principles for managing complex systems that could make it easier for investigators to share and reuse network components. We demonstrate the main challenges including incompatible levels of detail and ambiguous representation of complexes and highlight the need to address these challenges.
High-speed rotating device for X-ray tomography with 10 ms temporal resolution
Authors: Mashita Ryo , Yashiro Wataru , Kaneko Daisuke , Bito Yasumasa , Kishimoto Hiroyuki 
Published: December 2020

The temporal resolution of X-ray tomography, using a synchrotron radiation X-ray source, has been improved to millisecond order in recent years. However, the sample must be rotated at a speed of more than a few thousand revolutions per minute, which makes it difficult to control the environment around the sample. In this study, a high-speed rotation device has been developed, comprising two synchronized coaxial motors movable along the direction of the axis, which can stretch or compress the rotating sample. Using this device, tomograms of breaking rubber were successfully obtained at a temporal resolution of 10 ms.
Consolidating core entrepreneurial competences: toward a meta-competence framework
Authors: Reis Diane Aparecida , Fleury André Leme , Carvalho Marly Monteiro 
Published: December 2020

PurposeEmerging digital ventures and related breakthrough innovations result in new challenges for the development of entrepreneurial competences and demand new perspectives for entrepreneurship research. In this context, policy-makers and organizations are increasingly interested in fostering entrepreneurial competences to improve the success of policies and venture capital investments. This paper aims at identifying the core relevant entrepreneurial competences, mapping the current literature and the main clusters and going beyond toward a meta-competence framework.Design/methodology/approachThe research approach is a literature review, combining bibliometric, network and content analysis. The sampling process was conducted in the Scopus and Web of Science databases. The bibliometrics and content analysis were performed with a computer aid approach applying VosViewer1.6, Ucinet and NetDraw 2.139. The content analysis approach was performed considering a detailed coding schema developed. Finally, toward the meta-competences framework, the study applied quantitative analysis on the coding schema, particularly cross-tabulation, core-periphery and network analysis.FindingsThe results show the state of the art concerning entrepreneurial competences. The research identified a list of 98 entrepreneurial competences. Finally, the study proposes a meta-competence framework and clusters the 33 core entrepreneurial competences previously identified.Originality/valueThe proposed conceptual framework exploring meta-entrepreneurial competences offers an original contribution with implications for theory and practice. The research contributes to broadening the understanding of the entrepreneurial competences, helping on the creation, design, development and improvement of entrepreneurship educational initiatives, which are important to entrepreneurs' educators. The proposed framework contributes by providing relevant knowledge for the policy-makers' strategy formulation processes. As implications for the practice, the proposed framework can allow better assessment process for incubators and accelerators, besides more robust ventures considering learning trails based on meta-competences frameworks.
Realistic evaluation of the Flexmonitor, a team program to maintain and reflect on flexibility
Authors: van Gool Frank , Bierbooms Joyce , Janssen Richard , Bongers Inge 
Published: December 2020

PurposeFlexibility is necessary in a dynamic healthcare environment. However, balancing flexibility and consistency is difficult for healthcare teams, especially when working in threatening conditions. Methods are needed to help teams create, monitor and maintain flexibility.Design/methodology/approachThis study evaluates a practice-based program –– the Flexmonitor – which aims to help teams develop and maintain flexibility. Here, realistic evaluation was used to refine the program and define building blocks for future programs.FindingsThe Flexmonitor can be used to monitor implicit criteria and differences in interpretation and beliefs among team members to promote flexibility. It also monitors team behavior and the effects of this behavior on self-defined indicators. Using the Flexmonitor, team members can discuss their beliefs and the definitions and criteria of flexibility. Strikingly, teams were not able to effectively self-manage their flexibility using the Flexmonitor.Originality/valueThis article contributes to our knowledge of self-managing teams, particularly the question of whether team members can take responsibility for team flexibility.
Direct seminal fluid identification by protease‐free high‐resolution mass spectrometry
Authors: Brown Catherine O. , Robbins Brianna L. , McKiernan Heather E. , Danielson Phillip B. , Legg Kevin M. 
Published: December 2020

AbstractSerological screening of sexual assault evidence has traditionally focused on enzyme activity and immunochromatographic assays that provide only a presumptive indication of seminal fluid and have limited sensitivity relative to DNA testing. Seminal fluid detection based on protein mass spectrometry represents a “Next Gen” serological technology that overcomes the specificity and sensitivity limitations of traditional serological screening but requires time‐consuming sample preparation protocols. This paper describes a novel “peptidomics” approach to seminal fluid detection that eliminates the need for lengthy trypsin digestion. This streamlines sample preparation to a one‐step process followed by high‐resolution mass spectrometry to identify naturally occurring seminal fluid peptides and low‐molecular weight proteins. Multiple protein biomarkers of seminal fluid were consistently and confidently identified based on the multiplexed detection of numerous endogenous peptides. These included Semenogelin I and II (90% and 86% sequence coverage, respectively); Prostate Specific Antigen/p30 (29% sequence coverage); and Prostatic Acid Phosphatase (24% sequence coverage). The performance of this streamlined peptidomics approach to seminal fluid identification in a forensic context was also assessed using simulated casework samples of the type typically collected as part of a sexual assault examination (e.g., oral and vaginal swabs stained with semen). The resulting data demonstrate that sub‐microliter quantities of seminal fluid on cotton swabs can be recovered and reliably detected. This supports the forensic applicability of a peptidomic assay for seminal fluid identification with same‐day sample preparation and analysis. Future development and streamlined multiplex peptidomic assays for additional biological stains can easily be envisaged.
Spatial‐Temporal Distribution, Geological Characteristics and Ore‐Formation Controlling Factors of Major Types of Rare Metal Mineral Deposits in China
Authors: JIANG Shaoyong , SU Huimin , XIONG Yiqu , LIU Tao , ZHU Kangyu , ZHANG Lu 
Published: December 2020

AbstractRare metals including Lithium (Li), Beryllium (Be), Rubidium (Rb), Cesium (Cs), Zirconium (Zr), Hafnium (Hf), Niobium (Nb), Tantalum (Ta), Tungsten (W) and Tin (Sn) are important critical mineral resources. In China, rare metal mineral deposits are spatially distributed mainly in the Altay and Southern Great Xingán Range regions in the Central Asian orogenic belt; in the Middle Qilian, South Qinling and East Qinling mountains regions in the Qilian–Qinling–Dabie orogenic belt; in the Western Sichuan and Bailongshan–Dahongliutan regions in the Kunlun–Songpan–Garze orogenic belt, and in the Northeastern Jiangxi, Northwestern Jiangxi, and Southern Hunan regions in South China. Major ore‐forming epochs include Indosinian (mostly 200–240 Ma, in particular in western China) and the Yanshanian (mostly 120–160 Ma, in particular in South China). In addition, Bayan Obo, Inner Mongolia, northeastern China, with a complex formation history, hosts the largest REE and Nb deposits in China. There are six major rare metal mineral deposit types in China: Highly fractionated granite; Pegmatite; Alkaline granite; Carbonatite and alkaline rock; Volcanic; and Hydrothermal types. Two further types, namely the Leptynite type and Breccia pipe type, have recently been discovered in China, and are represented by the Yushishan Nb–Ta– (Zr–Hf–REE) and the Weilasituo Li–Rb–Sn–W–Zn–Pb deposits. Several most important controlling factors for rare metal mineral deposits are discussed, including geochemical behaviors and sources of the rare metals, highly evolved magmatic fractionation, and structural controls such as the metamorphic core complex setting, with a revised conceptual model for the latter.
Mineral Deposit Model of Cu–Fe–Au Skarn System in the Edongnan Region, Eastern China
Authors: XIE Guiqing , MAO Jingwen , ZHU Qiaoqiao , HAN Yingxiao , LI Wei , DUAN Chao , YE Hui 
Published: December 2020

AbstractCu and Fe skarns are the world's most abundant and largest skarn type deposits, especially in China, and Au‐rich skarn deposits have received much attention in the past two decades and yet there are few papers focused on schematic mineral deposit models of Cu–Fe–Au skarn systems. Three types of Au‐rich deposits are recognized in the Edongnan region, Middle–Lower Yangtze River metallogenic belt: ∼140 Ma Cu–Au and Au–Cu skarn deposits and distal Au–Tl deposits. 137–148 Ma Cu–Fe and 130–133 Ma Fe skarn deposits are recognized in the Edongnan region. The Cu–Fe skarn deposits have a greater contribution of mantle components than the Fe skarn deposits, and the hydrothermal fluids responsible for formation of the Fe skarn deposits involved a greater contribution from evaporitic sedimentary rocks compared to Cu–Fe skarn deposits. The carbonate‐hosted Au–Tl deposits in the Edongnan region are interpreted as distal products of Cu–Au skarn mineralization. A new schematic mineral deposit model of the Cu–Fe–Au skarn system is proposed to illustrate the relationship between the Cu–Fe–Au skarn mineralization, the evaporitic sedimentary rocks, and distal Au–Tl deposits. This model has important implications for the exploration for carbonate–hosted Au–Tl deposits in the more distal parts of Cu–Au skarn systems, and Fe skarn deposits with the occurrence of gypsum‐bearing host sedimentary rocks in the MLYRB, and possibly elsewhere.
Geochemistry and Geochronology of the Causative Intrusion and a New Genetic Model Study of the Yaojialing Polymetallic Skarn Deposit, Tongling District, China
Authors: XIONG Yanyun , ZHOU Taofa , FAN Yu , CHEN Jing , LIU Jun 
Published: December 2020

AbstractThe Yaojialing deposit is the first large‐scale Zn–Au–Cu polymetallic skarn deposit located in the Shatanjiao ore field of the Tongling area in the Middle–Lower Yangtze belt. It has distinct metallogenic characteristics and is clearly different from the known skarn Cu–Au deposits in the Tongling area and the Middle–Lower Yangtze belt. Previous studies of the Yaojialing deposit have included rock geochemistry, alteration and mineralization characteristics, as well as metallogenesis and metallogenic models. However, there are still numerous problems concerning the coexistence of multiple elements, metallogenetic specialization of the magma and the metallogenic model. In this study, using the latest production exploration work on the deposit, we investigated the Yaojialing deposit including its geological characteristics, petrography, LA–ICP MS zircon U–Pb dating and whole rock geochemistry. Two kinds of magmatic rocks have been distinguished for the first time in the deposit, amongst which the granodiorite porphyry exposed on the surface of the mining area, which is the host rock of the veined lead–zinc ore body, is the wall‐rock intrusion; and the deep concealed quartz monzonite porphyry is the causative intrusion, the distribution of orebodies and wall‐rock alteration characteristics showing regular zoning around the quartz monzonite porphyry. The 206Pb/238U weighted average age of the granodiorite porphyry is 140.2 ± 1.0 Ma (MSWD = 0.85, n = 13) by LA–ICP MS zircon U–Pb dating, while the quartz monzonite porphyry is 138.9 ± 1.2 Ma (MSWD = 0.60, n = 16), which is consistent with petrographic evidence. The geochemical characteristics show that the quartz monzonite porphyry is a high‐K calc‐alkaline series peraluminous rock. The trace element characteristics show that the quartz monzonite porphyry is enriched in LILE such as K, Rb, Sr, Ba and LREE, yet depleted in HFSE such as Nb, Ta, P and Ti. The Yaojialing deposit shows the mineralization characteristics of proximal skarn and distal skarn, having the common characteristics of ‘multi‐storey’ and ‘Trinity’ metallogenic models.
Trace Elements in Sphalerite from the Dadongla Zn‐Pb Deposit, Western Hunan–Eastern Guizhou Zn‐Pb Metallogenic Belt, South China
Authors: HU Yusi , YE Lin , WEI Chen , LI Zhenli , HUANG Zhilong , WANG Haoyu 
Published: December 2020

AbstractThe western Hunan–eastern Guizhou Zn‐Pb metallogenic belt is one of the important Zn‐Pb mineralization regions in China. The Dadongla deposit, located in the northeast of Guizhou Province, is one of the typical Zn‐Pb deposits in the region and has estimated resources more than 12 million metric tons (Mt) with an average grade of 4.11 wt% Zn+Pb. Its orebodies are hosted in the lower Cambrian Aoxi Formation dolomite, occurring as bedded, para‐bedded in shape, and in conformity with the wall rock. The ore mineral assemblage is simple, dominated by sphalerite with minor pyrite and galena, and the gangue minerals are composed of dolomite, calcite with minor bitumen and barite. In view of the lack of geological and geochemical researches, the genesis of Zn‐Pb ore is still unclear. Laser ablation‐inductively coupled plasma mass spectrometry (LA‐ICPMS) spot and mapping analyses were used to obtain sphalerite trace element chemistry in the Dadongla Zn‐Pb deposit in Guizhou, China, aiming to constrain its ore genesis. The results show that sphalerite is characterized by the enrichment of Cd, Fe, Ge and Hg, corresponding with that of typical MVT deposits. Four zones were identified in the sphalerite crystal from Dadongla from the center to margin according to the color bands, in which the zone in the center, representing the early ore‐stage sphalerite, is characterized by enrichment of Cd relatively, while the zone forming at late ore‐stage is enriched in Ge and Hg relatively. The finding was controlled by differential leached metals content in ore‐forming fluid from its source rock. Notably, critical element Ge trends to be enriched at the late ore‐stage and follows a substitution of 2Zn2+↔ Ge4++□ (vacancy). Moreover, the calculated ore‐forming temperature ranges from 79.9°C to 177.6°C by the empirical formula, which is similar to that of typical Mississippi Valley‐type (MVT) deposits. Compared with the features of trace elements in sphalerite from different types of deposits, together with the geology, the Dadongla deposit belongs to an MVT Zn‐Pb deposit.
Food‐dependent NSAID‐induced hypersensitivity (FDNIH) reactions: Unraveling the clinical features and risk factors
Authors: Sánchez‐López Jaime , Araujo Giovanna , Cardona Victoria , García‐Moral Alba , Casas‐Saucedo Rocío , Guilarte Mar , Torres María José , Doña Inmaculada , Picado Cesar , Pascal Mariona , Muñoz‐Cano Rosa , Bartra Joan 
Published: December 2020

AbstractBackgroundIn up to 70%–80% of patients with a suspected non‐steroidal anti‐inflammatory drug hypersensitivity (NSAIDH), challenge tests with the culprit drug yield negative results. On the other hand, there could be a NSAIDH overdiagnosis when anaphylaxis is the clinical manifestation. We hypothesize that some negative NSAID challenge tests and an overdiagnosis of NSAIDH occur in patients with food‐dependent NSAID‐induced hypersensitivity (FDNIH).MethodsWe studied 328 patients with a suspected acute NSAIDH. FDNIH was diagnosed in patients meeting all the following: (1) tolerance to the food ingested more temporally closed before the reaction, later the episode, (2) respiratory or cutaneous symptoms or anaphylaxis related to NSAID, (3) positive skin prick test to foods and/or specific IgE to food allergens (Pru p 3, Tri a 19, Pen a 1) involved in the reaction, and (4) negative oral provocation test to the culprit NSAID.Results199 patients (60%) were diagnosed with NSAIDH and 52 (16%) with FDNIH. Pru p 3 was involved in 44 cases (84.6%) and Tri a 19 in 6 cases (11%). FDNIH subjects were younger (p < .001), with a higher prevalence of rhinitis (p < .001) and previous food allergy (p < .001), together with a higher proportion of subjects sensitized to pollens (p < .001) and foods (p < .001). Using just four variables (Pru p 3 sensitization, Tri a 19 sensitization, anaphylaxis, and any NSAID different from pyrazolones), 95.3% of cases were correctly classified, with a sensitivity of 92% and specificity of 96%.ConclusionEvaluation of FDNIH should be included in the diagnostic workup of NSAIDH.