bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Digital Subtraction Angiography in Canine Hindlimb Arteriography
Authors: Chen Tiannan , Wang Xue , Wang Chengwei , Zhang Shiyong , Wang Jie 
Published: June 2020

Abstract
BACKGROUND: Research in the field of lower extremity vascular disease has increased in popularity over the years. In order to properly characterize and validate the effectiveness of interventions, in vivo experimentation in large animals is required. Thus, it is necessary to find a method to detect the shape and density of blood vessels in the lower extremities, which can evaluate and verify the effectiveness of the treatment measures, and has high feasibility and repeatability. This experiment is to characterize the influencing factors and determine both the feasibility and overall value of digital subtraction angiography in lower limb arteriography in a canine animal model.METHODS: Six Beagle dogs were immobilized on a working bed after administration of anesthesia. Percutaneous access was achieved via the femoral artery using an indwelling needle. A bolus of contrast agent was injected into the access site and digital subtraction angiography bolus chase technology was used to collect contrast images for analysis of this method.At the end of our procedure, the Beagles was anesthetized,veterinary injected excess potassium chloride.After confirming the loss of vital signs of beagles, the carcasses were handed over to the animal experimental center of Xinjiang Medical University,handled by qualified institutions.RESULT: The final arteriographic images of the hind limbs from all six canines were clear, and the branches of small vessels could be distinguished without any visible artifacts.CONCLUSION: These results suggest that arteriography using digital subtraction angiography can clearly reveal the shape and density of blood vessels in canine animal models. This method has great potential to significantly improve research related to limb ischemia, due to its simple and reproducible results.
Electrocardiographic findings of methanol toxicity: A cross-sectional study of 356 cases in Iran.
Authors: Nikoo Mohammad Hossein , Arjangzadeh Alireza , Pakfetrat Maryam , Boogar Shahrokh Sadeghi , Mohammadkarimi Vahid , Ostovan Vahid Reza , Khodamoradi Zohre , Roozbeh Jamshid , Khalili Mohammadreza , Shirazi Farnaz Kamali Haghighi , Kouhi Paryia , Heydari Seyyed Taghi 
Published: June 2020

Abstract
Bakground: Methanol is used widely in industry but methanol poisoning is not common; however, a number of outbreaks have been recently reported due to inappropriate processing of alcoholic beverages. Shiraz which is located in the southern part of Iran faced one of these in 2020 during COVID-19 pandemic. There is scarce literature on the electrocardiographic findings in methanol toxicity. The aim of this study is to address this gap in the literature. Method: A total of 356 cases with methanol toxicity were referred to Shiraz University of Medical Science Tertiary Hospitals (Faghihi and Namazi) in March and April 2020. The clinical findings such as blindness and impaired level of consciousness, lab data such as arterial blood gas, electrolytes, and creatinine, and the most common findings from ECGs were collected. Results: The most common ECG findings were J point elevation (68.8%), presence of U wave (59.2%), QTc prolongation (53.2% in males and 28.6% in females), and fragmented QRS (33.7%). An outstanding finding in this study was the presence of myocardial infarction in 5.3% of the cases, a finding which, to our knowledge, has only been reported in in a few case reports. Brugada pattern (8.1%) and Osborn wave (3.7%) were other interesting findings. In multivariate analysis, when confounding factors faded, myocardial infarction, atrioventricular conduction disturbances, sinus tachycardia and a prolonged QTC of more than 500 msecond were four independent correlates of methanol toxicity severity measured with arterial blood PH on arterial blood gas measurements, with p values and odds ratios of < 0.001 (12.386), 0.012 (5.981), 0.018(2.262) and 0.001(3.247), respectively. Conclusion: Electrocardiographic changes during methanol intoxication are remarkable and correlate well with the severity of poisoning. Myocardial infarction was an egregious yet concerningly common finding in this sample, which need to be ruled out in methanol toxicity.
Progesterone levels on the human chorionic gonadotropin trigger day affect the pregnancy rates for embryos transferred at different stages of development in both general and selected IVF/ICSI populations
Authors: Merviel Philippe , Bouée Sarah , Jacamon Anne-Solenn , Chabaud Jean-Jacques , Martelot Marie-Thérèse Le , Roche Sylvie , Rince Camille , Drapier Hortense , Perrin Aurore , Beauvillard Damien 
Published: June 2020

Abstract
Background
Two meta-analyses have shown that pregnancy and birth rates are significantly higher after blastocyst transfer than after cleaved embryo transfer. Other studies have revealed that a serum progesterone level > 1.5 ng/ml on the trigger day is responsible for premature luteinization and is associated with a low pregnancy rate. The objectives of the present study were to determine whether blastocyst transfer gave higher pregnancy rates than cleaved embryo transfer at day 3 in both the general and selected IVF/ICSI populations, and whether the serum progesterone level influenced the pregnancy rate.
Method
: We studied IVF/ICSI cycles with GnRH antagonist - FSH/hMG protocols in a general population (n = 1210) and a selected “top cycle” population (n = 677), after blastocyst transfer on D5 or cleaved embryo transfer on D3. The selected cycles had to meet the following criteria: female age < 35, first or second cycle, and one or two embryos transferred. We recorded predictive factors for pregnancy and calculated the serum progesterone to oocyte ratio (P/Ooc), the serum progesterone to serum estradiol ratio (P/E2), and the serum progesterone to follicle (> 14 mm) index (PFI).
Results
In the general population, the clinical pregnancy rate was significantly higher after blastocyst transfer (33.3%) than after cleaved embryo transfer (25.3%; p < 0.01). The differences between blastocyst and embryo transfer groups were not significant in the selected population (respectively 35.7% vs. 35.8% for the clinical pregnancy rate). The only predictive factors common to the general and selected populations were the serum progesterone levels on the eve of the trigger day and on the day itself, which were significantly lower in the subgroups of women who became pregnant (p < 0.01). We found a serum progesterone threshold of 0.9 ng/ml, as also reported by other studies. The P/Ooc ratio and the PFI appear to have predictive value for cleaved embryos transfers.
Conclusions
Blastocyst transfers were associated with higher clinical pregnancy rate than cleaved embryo transfers in a general population but not in a selected population. The serum progesterone levels on the eve of the trigger day and on the day itself predicted the likelihood of pregnancy.
Health-Related Behaviors and Health Insurance Status among US Adults: Findings from the 2017 Behavioral Risk Factor Surveillance System
Authors: Zhao Guixiang , Hsia Jason , Town Machell 
Published: June 2020

Abstract
Background
Health insurance coverage has increased overtime. However, little is known on the associations between health insurance status and adoption of health-related behaviors among US adults.
Methods
The 2017 Behavioral Risk Factor Surveillance System collected data on health insurance coverage in 50 states and the District of Columbia (DC), and on type of insurance in 5 states and DC. We examined four health-related behaviors: no tobacco use, nondrinking or moderate drinking, meeting aerobic physical activity recommendations, and having a healthy body weight. We conducted log-linear regression analyses to assess the associations between health-related behaviors and insurance status, while adjusting for potential confounders. Data analyses were conducted in 2019.
Results
The percentages of adults who reported no tobacco use or meeting physical activity recommendations were significantly higher, and the percentages of adults with a healthy body weight were significantly lower among those who were insured versus uninsured, or among adults with private insurance versus uninsured. Adults with health insurance also had a higher prevalence of reporting all 4 health-related behaviors than those uninsured. These patterns persisted after multivariable adjustment for potential confounders including sociodemographics, routine checkup, and number of chronic diseases. Adults with public insurance were 7% more likely to report no tobacco use than adults who were uninsured. Additionally, adults with private insurance were 8% and 7% more likely to report no tobacco use and meeting physical activity recommendations, respectively, but 10% less likely to report nondrinking or moderate drinking than adults with public insurance.
Conclusions
Significant associations existed between having health insurance coverage and engaging in some health-related behaviors among US adults.
Modified culture system and combination of FICTION could increase the detection rate of abnormalities in multiple myeloma
Authors: Fu Qiang , Xie Shanzhen , Chen Jiadi , Ye Changjiang , Huang Huifang 
Published: June 2020

Abstract
Background

Conventional karyotyping of multiple myeloma (MM) is hampered by the low mitotic index of plasma cells (PCs). Furthermore, low proportions of PCs in some specimens may lead to false negative results in fluorescence in situ hybridisation (FISH) detection. The detection rate of cytogenetic abnormalities in MM must therefore be improved.
Methods

Bone marrow cells were cultured in an ordinary medium for 24 h or in a medium containing 10 ng/mL IL-6 and 40 ng/mL GM-CSF for 6 d. Fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasms (FICTION) was also conducted, which combines fluorescence immunophenotyping (using CD138) and FISH.
Results

Under modified culture conditions, the successful rate of culture and abnormality rate during karyotype analysis increased to 86.4% and 40.9%, respectively. The abnormality rate of FICTION (89.5%) was significantly higher than that of FISH (60.0%). The genetic abnormality rate increased to 92.3% when FICTION and karyotyping were conducted under modified culture conditions.
Conclusion

The established modified culture system could improve karyotyping quality in MM. Owing to its obvious advantages compared with FISH, FICTION is recommended for detecting genetic abnormalities in MM. Combining modified culture system and FICTION could improve the sensitivity of cytogenetic risk stratification in MM patients.
Analysis of Normal Ankle Radiographic Angles
Authors: Zheng Weixin , Du Junhua , Lu Yao , Liang Jingqi , Zhang Yan , Liang Xiaojun , Zhao Hongmou 
Published: June 2020

Abstract
Background

Understanding the position of the normal ankle is the key before every successfully surgical planning. The aims of our study were to quantify the normal ankle angles, measures and reference points systematically on ankle radiographs.
Methods

A total of 900 angles and 300 basic information were measured on 100 healthy ankles. The radiographic measurements were performed on standard weight-bearing anteroposterior (AP) and lateral view of the ankle. A total of 6 angles were made from the AP view, 3 angles from the lateral view. All angles were measured by 2 foot and ankle surgeons, independent of each other. For intraobserver reliability, after six weeks, the same angles were measured by each observer.
Results

A total of 900 angles and 300 basic information were measured on 100 healthy ankles. The mean age of the subjects was 33.0 ± 9.1years, with 57 females(57%) and 43 males(43%), with 44 left feet(44%) and 56 right feet(56%). Intraobserver is reliability greater than 0.8 and the interobserver reliability greater than 0.8. The following angles were measured from the AP of ankle: TAS’s angle, TT’s angle, TC’s angle, TMM’s angle, TLM’s angle, MA’s angle. The following angles were measured from the lateral view: TLS’s angle, Kite angle, TTA’s angle.
Conclusions

Our study will provide a set of standard angle measures. The range of angles can be used as a reference for preoperative design and intraoperative and postoperative evaluations.
Patient Characteristics and Medical Provider Factors Are Associated With Length of Stay in Total Hip Arthroplasty Patients: a Retrospective Chinese Cohort Study
Authors: Zeng Hui , Li Guoqing , Yu Fei , Weng Jian , Xiong Ao , Fu Qiang , Zeng Yucong , Wang Fangxi , Xu Chang , Wang Deli 
Published: June 2020

Abstract
Background

Total hip arthroplasty (THA) is a successful treatment in the improvement of quality of life. Diagnosis-related groups (DRGs) payment has a significant impact on the hospital market in China and length of stay (LOS) is one of its crucial manifestations. Patient characteristics and medical provider factors can affect LOS but the relationship is uncertain. We intent to explore the relationship between patient characteristics and medical provider factors and LOS of primary THA patients.
Methods

We reviewed the database containing 461 patients who underwent primary THA between January 2014 to January 2019 and regressed the LOS against a variety of perioperative factors. A multivariable linear regression model was performed to assess the difference.
Results

For parts of patient characteristics, multivariable linear regression analysis revealed that comorbidities, pre-operation albumin < 30 g/L, and pre-operation CRP ≥ 5 mg/L were all significantly associated with LOS (p < 0.05). For parts of medical provider factors, multivariable linear regression analysis revealed that date of surgery, urinary catheter, and incision drainage were all significantly associated with LOS (p < 0.05).
Conclusions

Patient characteristics and medical provider factors are associated with LOS of THA patients. Evaluation and identification of risk factors are beneficial in patients' education, perioperative discussion and surgery decisions in the different primary THA patient populations.
Alteration of Screw-home Movement in Patients With Knee Osteoarthritis: A Cross-sectional Study
Authors: Jeon Jeongwoo , Lee Dongyeop , Yu Jaeho , Kim Jinseop , Lhee Sang Hoon , Chong Suri , Hong Jiheon 
Published: June 2020

Abstract
Background: Tibial rotation accompanying sagittal movement contains the phenomenon of screw-home movement of the knee, which plays an important role in knee stability during extension. This study aimed to investigate the alteration of screw-home movement in patients with knee osteoarthritis (OA).Methods: Participants (n =67) in this cross-sectional study were outpatients in the department of orthopedics of a general hospital and included patients with knee OA (n=31) and asymptomatic control subjects (n=36).Knee kinematic data were measured using an inertial measurement unit. The total tibial rotation was obtainedduring knee sagittal movement. The acquired angle of tibial rotation was divided into four periodseach extension and flexion. The total tibial rotation and the variation of each period were compared between the OA and control groups.Results: Significant differences arose between the OA and control groups in the total tibial rotation during knee extension and flexion(P< 0.001).The variation of tibial rotation was also significantly different between groups for all periods (P< 0.001; knee extensionat 70° to 45°,P = 0.014).Conclusions: We found a reduction in the total tibial rotation and loss of the screw-home movement in the unloaded OA knee. To normalize the screw-home movement, it is necessary to promote proper articular movement of the knee joint and suppress the hyperexcitability of the medial muscles.
Factors Associated with Failure of Bony Union After Conservative Treatment of Acute Cases of Unilateral Lumbar Spondylolysis
Authors: Tatsumura Masaki , Gamada Hisanori , Okuwaki Shun , Eto Fumihiko , Nagashima Katsuya , Ogawa Takeshi , Mammoto Takeo , Hirano Atsushi , Koda Masao , Yamazaki Masashi 
Published: June 2020

Abstract
Background: If bone union is expected, conservative treatment is generally selected for lumbar spondylolysis. However, sometimes conservative treatments are unsuccessful. We sought to determine the factors associated with failure of bony union in acute unilateral lumbar spondylolysis with bone marrow edema including contralateral pseudoarthrosis.Methods: This study targeted unilateral lumbar spondylolysis treated conservatively in high school or younger students. Conservative therapy was continued until the bone marrow edema disappeared on MRI and bone union was investigated by CT. We conducted a univariate analysis of sex, age, pathological stage, lesion level complicating the contralateral bone defect, lesion level, and intercurrent spina bifida occulta, and variables with p < 0.1 were considered in a logistic regression analysis. An item with p < 0.05 was defined as a factor associated with failure of bony union.Results: We found 92 cases of unilateral spondylolysis with bone marrow edema and 66 cases were successfully treated conservatively. Failure of bony union in unilateral lumbar spondylolysis with bone marrow edema was associated with advanced pathological stage (p = 0.004), contralateral pseudoarthrosis (p < 0.001), and L5 lesion level (p = 0.002). The odds ratio was 20.0 (95% CI 3.0–193.9) for advanced pathological stage, 78.8 (95% CI 13–846) for contralateral pseudoarthrosis, and 175 (95% CI 8.5–8192) for L5 lesion level.Conclusions: Conservative therapy aiming at bony union is contraindicated in cases of acute unilateral spondylolysis when the pathological stage is advanced, the lesion level is L5, or there is contralateral pseudoarthrotic spondylolysis.
Genomic surveillance of a resurgence of COVID-19 in Guangzhou, China
Authors: Zhang Zhou-Bin , Li Peng , Liu Hong-Jie , Zhong Jia-Yong , Zheng Yingfeng , Li Kui-Biao , Qin Peng-Zhe , Zeng Qing , Li Jin-Hui , Li Li-Zhong , Cao Lan , Wu Ji-Bin , Chen Yi-Yun , Jia Lei-Li , Song Hong-Bin , Wang Hua-Dong , Zhang Qi-Wei , Fan Jun , Di Biao , Jia Hong-Ling , Ni Ming , Xiao Chuan-Le 
Published: June 2020

Abstract
In the middle of March, the World Health Organization declared the outbreak of COVID-19 caused by SARS-CoV-2 infection a global pandemic. While China experienced a dramatic decline in daily growth rate of COVID-19, multiple importations of new cases from other countries and their related local infections caused a rapid rise. Between March 12 and April 15, we collected nasopharyngeal samples from 109 imported cases from 25 countries and 69 local cases in Guangzhou, China. In order to characterize the transmission patterns and genetic evolution of this virus among different populations, we sequenced the genome of SARS-CoV-2. The imported viral strains were assigned to lineages distributed in Europe (33.0%), America (17.4%), Africa (25.7%), or Southeast/West Asia (23.9%). Importantly, 10 imported cases from Africa formed two novel sub-lineages not identified in global tree previously. A detailed analysis showed that the imported viral strains from Philippines and Pakistan were closely related and within the same sub-lineage, whereas Ethiopia had varied lineages in the African phylogenetic tree. In spite of the diversity of imported SARS-CoV-2, 60 of 69 local infections could be traced back to two specific small lineages imported from Africa. A combined genetic and epidemiological analysis revealed a high-resolution transmission network of the imported SARS-CoV-2 in local communities, which might help inform the public health response and genomic surveillance in other cities and regions. Finally, we observed in-frame deletions on seven loci of SARS-CoV-2 genome, some of which were intra-host mutations, and they exhibited no enrichment on the S protein. Our findings provide new insight into the viral phylodynamics of SARS-CoV-2 and beta coronavirus.
MCL-1 plays an oncogenic role in breast cancer by modulating chemoresistance and stemness properties by activating Wnt/β-catenin
Authors: Zhang Yuzhu , Zhu Ling , Zhang Yanmei , Lu Hai , Wei Xiaoqing , Xu Rui , Ren Liping , Chen Qianjun 
Published: June 2020

Abstract
Background

Breast cancer is the most common malignant tumor and the leading cause of death in women. Chemotherapy is one of the most important treatments for breast cancer. However, the development of chemotherapy resistance is the main cause of 20–30% of breast cancer patients developing metastasis, leading to death. MCL-1, an anti-apoptotic protein, has not been found to contribute to chemotherapy resistance in breast cancer.
Methods

We used large gene panels to detect pathological sections of tumors in drug-resistant and sensitive patients. We validated protein profiling by IHC in a larger cohort of samples. We performed the function of MCL-1 by knockdown and overexpression in vitro and in vivo. Luciferase assay and CHIP assay were used to prove the regulatory network between MCL-1 and LRP6.
Result

We found that MCL-1 is more highly expressed in drug-resistant breast cancer tissues than it is in sensitive breast cancer tissues. Functional studies have revealed that MCL-1 plays an important role in drug resistance by regulating apoptosis in breast cancer cells. We found that overexpression of MCL-1 enhances the chemoresistance and stemness of breast cancer cells in vitro and in vivo, while silencing has the opposite effect. Mechanistically, by downregulating and upregulating MCL-1, we show that MCL-1 regulates LRP6 and activates the WNT/β-catenin signaling pathway in breast cancer cells. Finally, we found that a high level of MCL-1 expression predicts a poor prognosis in breast cancer.
Conclusion

Our work highlights the role of MCL-1 in chemoresistance and stemness. The MCL-1-WNT/β-catenin axis might be used as a new clinical target for breast cancer therapy.
Ethnic Disparities in Stillbirth Risk in Yunnan, China: A Prospective Cohort Study, 2010-2018
Authors: Wu Yanpeng , Pan Jianhong , Han Dong , Li Lixin , Wu Yanfei , Liao Rui , Liu Zijie , You Dingyun , Chen Pingyan , Wu Ying 
Published: June 2020

Abstract
Background: Racial and ethnic disparities in stillbirth risk had been documented in most western countries, but it remains unknown in China. This study was to determine whether exist ethnic disparities in stillbirth risk in mainland China. Methods: Data came from 217,070 pregnancies of Yunnan China during 2010-2018 year. The Han majority and other four main minorities including Yi, Dai, Miao, Hani were investigated in the analysis. The stillbirth hazards were estimated by life-table analysis. The excess stillbirth risk (ESR) was computed for Chinese minorities in multiple logistic regression. Results: Compared with other four minorities, women in Han majority were more likely to more educated, less multiparous, and less occupied in agriculture. The pattern of stillbirth hazard of Dai women across different gestation intervals were found to be different from other ethnic groups, especially in 20-23 weeks with 3.2 times higher than Han women. The ESR of the Dai, Hani, Miao, and Yi were 45.05%, 18.70%, -4.17% and 12.28%, respectively. Adjusted for maternal age, education, parity and other general risk factors, the ethnic disparity still persisted between Dai women and Han women. Adjusted for preterm birth further (gestation age <37 weeks) can reduce 19.39% ESR of Dai women and made the disparity insignificant. Maternal diseases and congenital anomalies explained little for ethnic disparities.Conclusions: We identified the ethnic disparity in stillbirth risk between Dai women and Han women. General risk factors including sociodemographic factors and maternal diseases explained little. Considerable ethnic disparities can be attributed to preterm birth.