bluuebunny/crossref_metadata_2025_split huggingface dataset:
High diatom species turnover in a Baltic Sea rock pool metacommunity
Authors: Aarnio Sonja , Teittinen Anette , Soininen Janne 
Published: November 2019

AbstractDifferent metacommunity perspectives have been developed to describe the relationship between environmental and spatial factors and their relative roles for local communities. However, only little is known about temporal variation in metacommunities and their underlying drivers. We examined temporal variation in the relative roles of environmental and spatial factors for diatom community composition among brackish-watered rock pools on the Baltic Sea coast over a 3-month period. We used a combination of direct ordination, variation partition, and Mantel tests to investigate the metacommunity patterns. The studied communities housed a mixture of freshwater, brackish, and marine species, with a decreasing share of salinity tolerant species along both temporal and spatial gradients. The community composition was explained by both environmental and spatial variables (especially conductivity and distance from the sea) in each month; the joint effect of these factors was consistently larger than the pure effects of either variable group. Community similarity was related to both environmental and spatial distance between the pools even when the other variable group was controlled for. The relative influence of environmental factors increased with time, accounting for the largest share of the variation in species composition and distance decay of similarity in July. Metacommunity organization in the studied rock pools was probably largely explained by a combination of species sorting and mass effect given the small spatial study scale. The found strong distance decay of community similarity indicates spatially highly heterogeneous diatom communities mainly driven by temporally varying conductivity gradient at the marine-freshwater transition zone.
Opposing the Power of Lies, Bullshit and Fake News: the Value of Truth
Authors: MacKenzie Alison , Bhatt Ibrar 
Published: November 2019

Abstract
‘Post-truth’ politics poses a serious challenge to the values of truth, and consequently trust. Sections of mainstream political parties and the media do not appear to have basic knowledge or insight into processes that underpin our institutions, and we are confronted with the proliferation of lies, fake news and bullshit—and profound ignorance. We will explore the distinctions between truth and truthfulness, and lies and deceit to centrally argue that truth has considerable intrinsic and instrumental value that should be protected and respected. Truth is invaluable to the integrity of the person, institution and nation, and a climate of trust to the proper functioning of democracy. While it may be expedient to distort or ignore the truth, we value truth in ways that cannot be reduced to its practical value. This is because it is very closely related to trust, sincerity and integrity. Indifference to truth, and the cynical espousing of lies, fake news or bullshit can be destabilising and harmful, as Brexit (the United Kingdom’s process of withdrawal from the European Union) is painfully revealing. Deception constitutes a limit to our will: how can we make informed choices if the information we receive is inaccurate, false or untrue? As our information ecosystem becomes increasingly complex, unpredictable and balkanised, educators have a vital role in helping an informed public navigate what it encounters online.
Forming a Transnational Moral Community between Soviet Dissidents and Ex-Communist Western Supporters: The Case of Pavel Litvinov, Karel van het Reve and Stephen Spender
Authors: Matsui Yasuhiro 
Published: November 2019

AbstractThe Soviet dissident and human rights movement, emerging publicly in the mid-1960s, became known globally through various supportive actions of Moscow foreign correspondents and Western intellectuals. For instance, Pavel Litvinov, a well-known dissident, had close relationships with foreigners, especially Karel van het Reve, a Dutch correspondent in Moscow, and Stephen Spender, a British poet, both formerly communists. This article attempts to elucidate aspects of the personal and ethical interactions among these three figures, focusing on van het Reve's and Spender's support activities and projects, particularly the Alexander Herzen Foundation and Writers and Scholars International, founded in 1969 and 1971, respectively, to understand how a transnational moral community was formed.
Perpetuating factors of social anxiety: a serial mediation model
Authors: Carnahan Nicolette D. , Carter Michele M. , Herr Nathaniel R. 
Published: November 2019

AbstractBackground:There is evidence that individuals with high levels of social anxiety utilize more safety behaviours and experience more post-event processing than those with lower levels of social anxiety. There are also data to suggest that the relationship between safety behaviour use and social anxiety symptoms is mediated by perceived control of one’s anxiety. Furthermore, it has been suggested that post-event processing influences anticipatory anxiety for a future social situation.Aim:A direct link between the perpetuating factors of social anxiety described above has not been established in the literature. The aim of the current study was to test a model examining the relationship between these constructs.Method:Participants first completed a battery of questionnaires. They then participated in an impromptu, 3-minute speech and were informed they would be videotaped. Following the speech, participants completed measures of anxiety and were instructed to return the following week. During the second session, they were informed they would deliver an additional speech and provided ratings of their anxiety in anticipation of delivering the second speech.Results:The results of a serial mediation support that greater levels of social anxiety lead to less perceived control over one’s anxiety, leading to increased safety behaviour use. The increase in safety behaviours led to an increase of post-event processing which resulted in greater anticipatory anxiety for a future speech task.Conclusions:This study provides novel evidence for the importance of perceived control in the genesis of social anxiety, which has implications for treatment.
Nine axes of merit for technosignature searches
Authors: Sheikh Sofia Z. 
Published: November 2019

AbstractIt can be difficult to develop an effective and balanced search strategy in SETI, especially from a funding perspective, given the diverse methodologies and myriad orthogonal proposals for the best technosignatures. Here I propose a framework to compare the relative advantages and disadvantages of various proposed technosignatures based on nine ‘axes of merit’. This framework was first developed at the NASA Technosignatures Workshop in Houston in 2018 and published in that report. I give the definition and rationale behind the nine axes as well as the history of each axis in the SETI and technosignature literature. These axes are then applied to three classes of technosignature searches as an illustration of their use. An open-source software tool is available to allow technosignature researchers to make their own version of the figure.
Anthony Del Genio: Climates of Planets Near and Far
Authors: Del Genio Anthony D. 
Published: November 2019

Plain language summaryThis essay describes a career that has spanned one of the most momentous periods in science history, a time when humankind first ventured into space, visited every planet in the Solar System, discovered thousands of planets orbiting other stars, and during this whole time, began to unintentionally transform the climate of our own planet. The author had the opportunity to do research in all these areas—after failing an early graduate school exam—and grew as a scientist along the way as the direct result of working across disciplines, with the help of many colleagues whose talents complemented and often exceeded his own.
Numerical Simulation of the Irrigation Effects on Surface Fluxes and Local Climate in Typical Mountain‐Oasis‐Desert Systems in the Central Asia Arid Area
Authors: Zhang Miao , Luo Geping , Cao Xiaoshu , Hamdi Rafiq , Li Tao , Cai Peng , Ye Hui , He Huili 
Published: November 2019

AbstractIn this study, the Weather Research and Forecasting model was coupled with an improved Noah land surface model (WRF‐Noah) where dynamic flood and drip irrigation processes were implemented firstly. We simulated the different irrigation effects on surface water‐heat processes in a typical mountain‐oasis‐desert system in Central Asia for both wet and drought years, respectively, using the modified WRF‐Noah. The modified WRF‐Noah model can dynamically generate amounts of irrigation in agreement with actual values. The statistically significant decrease in the root mean square error and increase in the Pearson correlation coefficient for the 2‐m temperature (T2), relative humidity (RH), latent heat flux (LE), and precipitation suggest that the modified WRF‐Noah model was improved by implementing irrigation processes. During the irrigation season, flood and drip irrigation decreased the average sensible heat flux by −80.69 and −50.50 W/m2 and T2 by 1.09 and 0.82 °C over the irrigated area and increased LE by 88.47 and 66.70 W/m2 and RH by 6.23% and 4.65%, respectively. Throughout the domain, flood irrigation had equivalent or slightly weaker effects on near‐surface temperature and humidity due to the smaller irrigated area. Both flood and drip irrigation increased precipitation throughout the domain, especially in the mountainous area, thereby accelerating the hydrological cycle in the mountain‐oasis‐desert system. The local oasis breeze circulation that plays a role in maintaining the oasis stability is still counteracted by the dominant background circulation even with irrigation processes. Thus, more effort should be exerted to maintain the future sustainability of the oasis.
Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery
Authors: Wang Pengwei , Pleskot Roman , Zang Jingze , Winkler Joanna , Wang Jie , Yperman Klaas , Zhang Tong , Wang Kun , Gong Jinli , Guan Yajie , Richardson Christine , Duckney Patrick , Vandorpe Michael , Mylle Evelien , Fiserova Jindriska , Van Damme Daniel , Hussey Patrick J. 
Published: November 2019

AbstractThe Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these proteins are also involved in actin cytoskeleton regulated autophagy. Both AtEH/Pan1 proteins localise to the plasma membrane and autophagosomes. Upon induction of autophagy, AtEH/Pan1 proteins recruit TPC and AP-2 subunits, clathrin, actin and ARP2/3 proteins to autophagosomes. Increased expression of AtEH/Pan1 proteins boosts autophagosome formation, suggesting independent and redundant pathways for actin-mediated autophagy in plants. Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion, we identify the existence of an autophagy-dependent pathway in plants to degrade endocytic components, starting at the EPCS through the interaction among AtEH/Pan1, actin cytoskeleton and the EPCS resident protein VAP27-1.
Homologous bd oxidases share the same architecture but differ in mechanism
Authors: Theßeling Alexander , Rasmussen Tim , Burschel Sabrina , Wohlwend Daniel , Kägi Jan , Müller Rolf , Böttcher Bettina , Friedrich Thorsten 
Published: November 2019

AbstractCytochrome bd oxidases are terminal reductases of bacterial and archaeal respiratory chains. The enzyme couples the oxidation of ubiquinol or menaquinol with the reduction of dioxygen to water, thus contributing to the generation of the protonmotive force. Here, we determine the structure of the Escherichia coli bd oxidase treated with the specific inhibitor aurachin by cryo-electron microscopy (cryo-EM). The major subunits CydA and CydB are related by a pseudo two fold symmetry. The heme b and d cofactors are found in CydA, while ubiquinone-8 is bound at the homologous positions in CydB to stabilize its structure. The architecture of the E. coli enzyme is highly similar to that of Geobacillus thermodenitrificans, however, the positions of heme b595 and d are interchanged, and a common oxygen channel is blocked by a fourth subunit and substituted by a more narrow, alternative channel. Thus, with the same overall fold, the homologous enzymes exhibit a different mechanism.
The browning and re-browning of lakes: Divergent lake-water organic carbon trends linked to acid deposition and climate change
Authors: Meyer-Jacob Carsten , Michelutti Neal , Paterson Andrew M. , Cumming Brian F. , Keller Wendel , Smol John P. 
Published: November 2019

AbstractDissolved organic carbon (DOC) concentrations and water colour are increasing in many inland waters across northern Europe and northeastern North America. This inland-water “browning” has profound physical, chemical and biological repercussions for aquatic ecosystems affecting water quality, biological community structures and aquatic productivity. Potential drivers of this “browning” trend are complex and include reductions in atmospheric acid deposition, changes in land use/cover, increased nitrogen deposition and climate change. However, because of the overlapping impacts of these stressors, their relative contributions to DOC dynamics remain unclear, and without appropriate long-term monitoring data, it has not been possible to determine whether the ongoing “browning” is unprecedented or simply a “re-browning” to pre-industrial DOC levels. Here, we demonstrate the long-term impacts of acid deposition and climate change on lake-water DOC concentrations in low and high acid-deposition areas using infrared spectroscopic techniques on ~200-year-long lake-sediment records from central Canada. We show that acid deposition suppressed naturally higher DOC concentrations during the 20th century, but that a “re-browning” of lakes is now occurring with emissions reductions in formerly high deposition areas. In contrast, in low deposition areas, climate change is forcing lakes towards new ecological states, as lake-water DOC concentrations now often exceed pre-industrial levels.
Effects of human-made resource hotspots on seasonal spatial strategies by a desert pitviper
Authors: DeSantis Dominic L. , Wagler Amy E. , Mata-Silva Vicente , Johnson Jerry D. 
Published: November 2019

AbstractHabitat heterogeneity and local resource distribution play key roles in animal search patterns. Optimal strategies are often considered for foraging organisms, but many of the same predictions are applicable to mate searching. We quantified movement and space use by a pitviper to test whether Native Habitats (NH) and human-made Resource Hotspots (RH) facilitate alternative seasonal spatial strategies as a result of critical resources, including potential mating partners, being widely dispersed in NH and clustered in RH. Independent of habitat category, seasonal patterns resembled an intermediate mating system with elements of prolonged male mate-searching and female-defense. However, individuals using primarily NH or RH exhibited alternative strategies. NH rattlesnakes displayed greater movement and larger home ranges than RH rattlesnakes across behavioral seasons. NH males increased movement distances and home ranges during the mating season, while RH males displayed minimal or no seasonal shifts. NH females also elevated movement distances during the mating season, while RH females showed no significant seasonal differences. Despite contrasting spatial patterns, mating success and female-defense effort were not significantly affected by habitat category. This unique study system highlights the potential for interactions among sexual selection, habitat heterogeneity, and behavioral plasticity to facilitate divergent search tactics within populations.
Impact of the 2018 ASCO/CAP guidelines on HER2 fluorescence in situ hybridization interpretation in invasive breast cancers with immunohistochemically equivocal results
Authors: Wang Bo , Ding Wei , Sun Ke , Wang Xiaoling , Xu Liming , Teng Xiaodong 
Published: November 2019

AbstractThe American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) recently issued updated guidelines on human epidermal growth factor receptor 2 (HER2) testing by fluorescence in situ hybridization (FISH) in invasive breast cancers. In this study, we aimed to investigate the impact of the new recommendations on HER2 FISH interpretation in invasive breast cancers with immunohistochemically (IHC) equivocal results. 1810 breast cancer cases with IHC equivocal results were enrolled in this study between January 2012 and May 2019. Concomitant IHC was performed on the same tissue blocks detected by FISH testing. According to the 2018 guidelines, all the cases in ISH group 2 were categorized as HER2 negative; three of four cases in ISH group 3 were considered as HER2 positive, while the one scored IHC 1+ was reclassified as HER2 negative; Fifty-three previously ISH equivocal cases were redistributed into ten HER2-positive cases and forty-three HER2-negative cases. In conclusion, the utility of 2018 ASCO/CAP guidelines resulted in a slight decrease in HER2 positive rate, due to the reclassification of cases in ISH group 2 and group 4. The implementation of the new guidelines can reduce reflex FISH test and make the diagnosis of HER2 gene status more definitive.
Direct Conversion of Human Urine Cells to Neurons by Small Molecules
Authors: Xu Guosheng , Wu Feima , Gu Xiaotong , Zhang Jiaye , You Kai , Chen Yan , Getachew Anteneh , Zhuang Yuanqi , Zhong Xiaofen , Lin Zuoxian , Guo Dongsheng , Yang Fan , Pan Tingcai , Wei Hongcheng , Li Yin-xiong 
Published: November 2019

AbstractTransdifferentiation of other cell type into human neuronal cells (hNCs) provides a platform for neural disease modeling, drug screening and potential cell-based therapies. Among all of the cell donor sources, human urine cells (hUCs) are convenient to obtain without invasive harvest procedure. Here, we report a novel approach for the transdifferentiation of hUCs into hNCs. Our study demonstrated that a combination of seven small molecules (CAYTFVB) cocktail induced transdifferentiation of hUCs into hNCs. These chemical-induced neuronal cells (CiNCs) exhibited typical neuron-like morphology and expressed mature neuronal markers. The neuronal-like morphology revealed in day 1, and the Tuj1-positive CiNCs reached to about 58% in day 5 and 38.36% Tuj1+/MAP2+ double positive cells in day 12. Partial electrophysiological properties of CiNCs was obtained using patch clamp. Most of the CiNCs generated using our protocol were glutamatergic neuron populations, whereas motor neurons, GABAergic or dopaminergic neurons were merely detected. hUCs derived from different donors were converted into CiNCs in this work. This method may provide a feasible and noninvasive approach for reprogramming hNCs from hUCs for disease models and drug screening.
Orexin A as a modulator of dorsal lateral geniculate neuronal activity: a comprehensive electrophysiological study on adult rats
Authors: Orlowska-Feuer Patrycja , Smyk Magdalena Kinga , Palus-Chramiec Katarzyna , Dyl Katarzyna , Lewandowski Marian Henryk 
Published: November 2019

AbstractOrexins (OXA, OXB) are hypothalamic peptides playing crucial roles in arousal, feeding, social and reward-related behaviours. A recent study on juvenile rats suggested their involvement in vision modulation due to their direct action on dorsal lateral geniculate (dLGN) neurons. The present study aimed to verify whether a similar action of OXA can be observed in adulthood. Thus, in vivo and in vitro electrophysiological recordings on adult Wistar rats across light-dark and cortical cycles were conducted under urethane anaesthesia. OXA influenced ~28% of dLGN neurons recorded in vivo by either excitation or suppression of neuronal firing. OXA-responsive neurons did not show any spatial distribution nor represent a coherent group of dLGN cells, and responded to OXA similarly across the light–dark cycle. Interestingly, some OXA-responsive neurons worked in a cortical state-dependent manner, especially during the dark phase, and ‘preferred’ cortical activation over slow-wave activity induced by urethane. The corresponding patch clamp study confirmed these results by showing that < 20% of dLGN neurons were excited by OXA under both light regimes. The results suggest that OXA is involved in the development of the visual system rather than in visual processes and further implicate OXA in the mediation of circadian and arousal-related activity.
Global diversity and genetic landscape of natural populations and hatchery stocks of largemouth bass micropterus salmoides across American and Asian regions
Authors: Wang Dan , Yao Hong , Li Yan-He , Xu Yong-Jiang , Ma Xu-Fa , Wang Han-Ping 
Published: November 2019

AbstractAlthough largemouth bass Micropterus salmoides has shown its extremely economic, ecological, and aquacultural significances throughout the North American and Asian continents, systematic evaluation of genetic variation and structure of wild and cultured populations of the species is yet to be documented. In this study, we investigated the genetic structure of M. salmoides from 20 wild populations and five cultured stocks across the United States and China using eight microsatellite loci, which are standard genetic markers for population genetic analysis. Our major findings are as follows: (1) the result of Fst showed largemouth bass had high genetic differentiation, and the gene flow indicated the genetic exchange among wild populations is difficult; (2) AMOVA showed that 14.05% of the variation was among populations, and 85.95% of the variation was within populations; (3) The majority of largemouth bass populations had a significant heterozygosity excess, which is likely to indicate a previous population bottleneck; (4) Allelic richness was lower among cultured populations than among wild populations; (5) Effective population size in hatcheries could promote high levels of genetic variation among individuals and minimize loss of genetic diversity; China’s largemouth bass originated from northern largemouth bass of USA. The information provides valuable basis for development of appropriate conservation policies for fisheries and aquaculture genetic breeding programs in largemouth bass.