bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Sociodemographic and Information Factors on Family Behaviour in Early Detection of High-Risk Pregnancy
Authors: Mardiyanti Ika , Devy Shrimarti Rukmini , Ernawati Ernawati 
Published: March 2020

Introduction: Pregnancy and childbirth are physiological processes experienced by women, but they sometimes have risky conditions. There are still many pregnant women and their families who are unable to detect a high-risk of pregnancy early. This study aims to determine family behaviour in conducting early detection of a high-risk of pregnancy in terms of sociodemographic and information factors.Methods: This study uses an explanatory survey design with a cross sectional design. The sample size of this study was 146, with simple random sampling. The independent variables were sociodemographic factors (age, gender, ethnicity, education, income and religion) and information factors (experience, and media exposure) while the dependent variable is family behaviour. The instrument with the questionnaire used the Likert scale. The data was analysed using partial least square.Results: The results showed that Structural Equation Modelling-Partial least square (SEM-PLS) statistical analysis, through Confirmatory Factor Analysis (CFA), obtained sociodemographic factors on family behaviour of 1,999, and information factors on family behaviour of 13,78. The value of the influence of sociodemographic factors (0.102) and the value of the influence of information factors (0.754). R2 (0.63) and Q2 value of 0.65.Conclusion: Sociodemographic factors and information factors significantly influenced family behaviour factors in early detection of high-risk of pregnancy. Information factors have a greater effect on family behaviour than sociodemographic factors. Midwives as health service providers at the health care centre need to optimize family empowerment through health information efforts in health promotion efforts. Further research requires the involvement of other factors to improve family behaviour, especially in the ability of families to detect early high-risk pregnancies.
Ethnic Foods Diet Program Improve Self-Efficacy and Diet Compliance Among Type 2 Diabetic Patients
Authors: Has Eka Mishbahatul Mar'ah , Aulia Amira , Kusumaningrum Tiyas , Efendi Ferry 
Published: March 2020

A well-balanced diet is one of the four pillars of diabetes self-management. Patient's culture strongly influences intake food. Diabetic dietary guidelines which fit with the patient's culture is expected to improve patient's self-efficacy and diet compliance. This study was aimed to analyze the effect of ethnic foods diet program in improving self-efficacy and diet compliance among Type-2 Diabetes Mellitus (T2DM) patients. This was quasy experiment research with pre and post-test control design. The population was 112 T2DM patients from Sasak Tribes, West Nusa Tenggara. Samples were 36 respondents, divided into intervention (18) and control (18) groups. The independent variable was the ethnic food diet (EFD) program, while the dependent variables were patient's self-efficacy and diet compliance. Data were collected using self-efficacy questionnaire and a 24-hour dietary recall form. Data were then analyzed using Wilcoxon Signed Rank Test and Mann Whitney U Test. The result showed differences in self-efficacy between pre and post-test in the treatment group (p=0,001), but there were no differences in the control group. There were differences in diet compliance in the treatment group (p=0,001), but there were no differences in the control group. There were differences between treatment and control groups on self-efficacy (p=0,000) and diet compliance (p=0,000). Ethnic foods diet program can improve self-efficacy and diet compliance among T2DM patients because more comfortable and easier to be applied. Nurses can apply ethnic foods diet program as an intervention to promote healthy diet for T2DM patients.
Pengembangan Bahan Ajar Online (Learning Object) pada Matakuliah Landasan Teknologi Pendidikan
Authors: Fauzan Achmad Nur , Siregar Eveline , Imbar Kunto 
Published: March 2020

Penelitian pengembangan ini bertujuan menghasilkan bahan ajar yang bersifat non-cetak yang dimasukkan kedalam http://fip.unj.ac.id/hylearn/. Sasaran pengguna bahan ajar online ini ialah mahasiswa semester baru di TP UNJ. Penelitian pengembangan ini dilakukan berdasarkan model Rapid Prototyping yang memiliki lima tahapan yaitu tahap assess needs and analyze content (penilaian kebutuhan dan analisis konten), set objectives (menetapkan tujuan pembelajaran), constructing a prototype (mengembangkan prototype), utilizing the prototype (memanfaatkan prototipe (research/penelitian), dan installing the final system (memasang sistem). Dalam Evaluas itersebut melibatkan 1 orang ahli materi, 1 orang ahli media, dan 22 orang pengguna. Nilai rata-rata yang diperoleh dari tahap expert review oleh ahli materi ialah 3,36 dan menunjukkan kategori baik. Nilai rata-rata yang diperoleh dari tahap expert review oleh ahli desain pembelajaran ialah 3,4. Nilai rata-rata yang diperoleh dari tahap expert review oleh ahli media ialah 3,4 dan menunjukkan kategori baik. Pada tahap penggunaan face-to-face diperoleh nilai rata-rata 3,05 yang menandakan secara keseluruhan panduan ini cukup baik. Sedangkan secara small group diperoleh nilai rata rata 3,10 yang menandaakan penggunaan bahan ajar online tersebut dinilai baik.
A novel insight into nitrogen and auxin signaling in lateral root formation in tea plant [Camellia sinensis (L.) O. Kuntze]
Authors: Hu Shunkai , Zhang Mi , Yang Yiqing , Xuan Wei , Zou Zhongwei , Arkorful Emmanuel , Chen Yi , Ma Qingping , Jeyaraj Anburaj , Chen Xuan , Li Xinghui 
Published: March 2020

Abstract
Abstract
Background
Tea plant (Camellia sinensis) is one of the most popular non-alcoholic beverage worldwide. Lateral roots (LRs) of tea plant are the main organ used for tea plant to absorb soil moisture and nutrients. Lateral roots formation and development are tightly regulated by the nitrogen and auxin signaling pathway. In order to understand the function of auxin and nitrogen signaling in LRs formation and development, transcriptome analysis was applied to investigate the differentially expressed genes involved in lateral roots of tea plants treated with indole-3-butyric acid (IBA), N-1-naphthylphthalamic acid (NPA), low and high nitrogen concentration.
Results
A total of 296 common differentially expressed genes were mainly identified and annotated to four signaling pathways, such as nitrogen metabolism, plant hormone signal transduction, Glutathione metabolism and transcription factors. RNA-sequencing results revealed that majority of differentially expressed genes play important roles in nitrogen metabolism and hormonal signal transduction. Low nitrogen condition induced the biosynthesis of auxin and accumulation of transcripts, thereby regulating lateral roots formation. Furthermore, metabolism of cytokinin and ethylene biosynthesis were also involved in lateral roots development. Transcription factors like MYB genes also contributed to the lateral roots formation of tea plants through secondary cell wall biosynthesis. Reversed phase ultra performance liquid chromatography (RP-UPLC) results showed that the auxin concentration in lateral roots was increased, while the nitrogen level decreased. Thus, tea plant lateral roots formation could be induced by low nitrogen concentration via auxin biosynthesis and accumulation.
Conclusion
This study provides new insights into the mechanisms associated with nitrogen and auxin signaling pathways to regulate LRs formation and arises new clues for the efficient utilization of nitrogen in tea plant at the genetic level.
Spatio-temporal distribution and associated factors of home delivery in Ethiopia. Further multilevel and spatial analysis of Ethiopian Demographic and Health Surveys 2005 - 2016
Authors: Tessema Zemenu Tadesse , Tiruneh Sofonyas Abebaw 
Published: March 2020

Abstract
Background: Globally, between 2012 -2017, 80% of live births occurred at health facilities assisted by skilled health personnel. In Ethiopia, in 2016 only 26% of live births attended by skilled health personal. The aim of this study was to assess the spatial patterns and associated factors of home delivery in Ethiopia using 2005, 2011, and 2016 Ethiopian Demographic and Health Surveys. Method: A total of 33 482 women who gave live birth in the five years preceding each survey were included for this study. ArcGIS version 10.7 software was used to visualize the spatial distribution of home delivery. The Bernoulli model was applied using Kilduff SaTScan version 9.6 software to identify significant purely spatial clusters for home delivery in Ethiopia. A multilevel logistic regression model was fitted to identify factors associated with home delivery. A p-value < 0.05 was taken to declare statistically significant predictors. Result: Home delivery was declined from 94.78% in 2005, 90.05% in 2011, and 73.44% in 2016 in Ethiopia. Among the three surveys, consistently high clustering of home delivery was observed in Amhara and Southern Nations Nationalities and People’s Regions (SNNPR) of Ethiopia. In spatial scan statistics analysis, a total of 128 clusters (RR= 1.04, P-value < 0.001) in 2005, and 90 clusters (RR = 1.11, P-value < 0.001) in 2011, and 55 clusters (RR = 1.29, P-value < 0.001) in 2016 significant primary clusters were identified. Educational status of women and husband, religion, distance to the health facility, mobile access, antenatal care visit, birth order, parity, wealth index, residence, and Region were statistically significant predictors of home delivery.Conclusion: The spatial distribution of home delivery among the three consecutive surveys were non-random in Ethiopia. Educational status of women and husband, religion, distance to the health facility, wealth index, residence, parity, mobile access, Region, and antenatal care visit were statistically significant predictors of home delivery in Ethiopia. Therefore, an intervention needs to improve the coverage of antenatal care visit, and health care facilities. Ministry of health and other stakeholders should give special attention to women living in Amhara and SNNPR states of Ethiopia.
LipoDDx: a mobile application for identification of rare lipodystrophy syndromes
Authors: Araujo-Vilar David , Fernández-Pombo Antia , Rodríguez-Carnero Gemma , Martínez-Olmos Miguel Angel , Cantón Ana , Villar-Taibo Rocio , Hermida-Ameijeiras Alvaro , Santamaría-Nieto Alicia , Díaz-Ortega Carmen , Martínez-Rey Carmen , Antela Antonio , Losada Elena , Muy-Pérez Andrés , González-Méndez Blanca , Sánchez-Iglesias Sofía 
Published: March 2020

Abstract
Background: Lipodystrophy syndromes are a group of disorders characterized by a loss of adipose tissue once other situations of nutritional deprivation or exacerbated catabolism have been ruled out. With the exception of the HIV-associated lipodystrophy, they have a very low prevalence, which together with their large phenotypic heterogeneity makes their identification difficult, even for endocrinologists and pediatricians. This leads to significant delays in diagnosis or even to misdiagnosis. Our group has developed an algorithm that identifies the more than 40 rare lipodystrophy subtypes described to date. This algorithm has been implemented in a free mobile application, LipoDDx®. Our aim was to establish the effectiveness of LipoDDx®.40 clinical records of patients with a diagnosis of certainty of most lipodystrophy subtypes were analyzed, including subjects without lipodystrophy. The medical records, blinded for diagnosis, were evaluated by 13 physicians, 1 biochemist and 1 dentist. Each evaluator first gave his/her results based on his/her own criteria. Then, a second diagnosis was given using LipoDDx®. The results were analysed based on a score table according to the complexity of each case and the prevalence of the disease.Results: LipoDDx® provides a user-friendly environment, based on usually dichotomous questions or choice of clinical signs from drop-down menus. The final result provided by this app for a particular case can be a low/high probability of suffering a particular lipodystrophy subtype. Without using LipoDDx® the success rate was 17 ± 20%, while with LipoDDx® the success rate was 79 ± 20% (p <0.01).Conclusions: LipoDDx® is a free app that enables the identification of subtypes of rare lipodystrophies, which in this small cohort has around 80% effectiveness, which will be of help to doctors who are not experts in this field. However, it will be necessary to analyze more cases in order to obtain a more accurate efficiency value.
Feasibility of Heavy Slow Resistance Training in patients with subacromial shoulder pain. A single-blind, randomized pilot study.
Authors: Kvalvaag Elisabeth , Høiland Hans Hugo Fredriksen , Johnsen Marianne Bakke , Brox Jens Ivar , Engebretsen Kaia Beck , Røe Cecilie 
Published: March 2020

Abstract
Background: Subacromial shoulder pain is the most common shoulder diagnosis, and should preferably be treated non-operatively. Previous studies have shown promising results of Heavy Slow Resistance training (HSR) in patellar and Achilles tendinopathy, but few studies have evaluated the effect of HSR for shoulder pain. The purpose of this pilot trial was to evaluate if HSR training is feasible for patients with subacromial shoulder pain, and to compare HSR to traditional supervised exercises.
Methods: Twenty-two patients with subacromial shoulder pain lasting at least three months were recruited and randomized to HSR (n=11) or traditional supervised exercises (n=11) by computer-generated randomization in blocks of four. Patients received HSR once a week, in addition to home-training twice a week, or supervised exercises twice a week for 12 weeks, in addition to daily home-training. The primary outcome measure was the Shoulder Pain and Disability Index (SPADI). Linear regression analysis was applied to evaluate the between group differences after 12 weeks. Outcome assessors were masked.
Results: After 12 weeks, patients in both groups had improved significantly from baseline in SPADI score (P=0.001) but no group difference was found (mean difference 1.3; 95% CI, -21.9 to 24.5, P=0.91). Similar non-significant results between groups were seen for pain in activity and rest, and the DASH score.
Conclusion: This pilot-study observed similar results of HSR compared to supervised exercises for patients with subacromial shoulder pain and both groups improved significantly from baseline. Only one patient changed from HSR to supervised exercises due to increased pain. Based on this, HSR appears to be a feasible treatment for this patient group, but due to small sample size there is a risk of type II error and studies with larger sample size are required.
Mitochondrial tRNA methylation in Alzheimer’s disease and progressive supranuclear palsy
Authors: Silzer Talisa K. , Pathak Gita A. , Phillips Nicole 
Published: March 2020

Abstract
Background

Methylation of mitochondrial tRNAs (mt-tRNA) at the 9 th position (“p9 site”) is known to impact translational efficiency and downstream mitochondrial function; however, direct assessment of mt-RNA methylation is challenging. Recent RNA sequence-based methods have been developed to reliably identify post-transcriptional methylation. Though p9 methylation has been studied in healthy human populations and in the context of cancer, it has not yet been analyzed in neurodegenerative disease, where mitochondrial dysfunction is a prominent and early hallmark of disease progression.

Results

Here we show variable methylation at 11 p9 sites in post-mortem cerebellar tissue of elderly subjects who were either healthy or diagnosed with Alzheimer’s disease (AD), progressive supranuclear palsy (PSP) or pathological aging (PA). Similarities in degree of methylation were observed between AD and PSP. Expression of 5300 genes was significantly correlated with p9 methylation, with AD and PSP subjects exhibiting similar expression profiles. The top 100 transcripts were found to be enriched for mitochondrial-related toxicity. Overrepresentation testing using the top 1000 transcripts revealed enrichment for a number of molecular processes and terms including some which were mitochondrial-related.

Conclusion

With mitochondrial dysfunction being an established hallmark of neurodegenerative disease pathophysiology, this work sheds light on the potential molecular underpinnings of this dysfunction. Here we show overlap in cerebellar pathophysiology between common tauopathies such as Alzheimer’s disease and progressive supranuclear palsy. Whether p9 hypermethylation is a cause or consequence of pathology remains an area of focus.
Percutaneous endoscopic posterior lumbar interbody fusion(PEPLIF): technical note
Authors: He Liming , Feng Haoyu , Ma Xun , Chang Qiang , Chang Jianjun , Sun Lin 
Published: March 2020

Abstract
Background: Endoscopic lumbar interbody fusion is based on endoscopic lumbar discectomy, a well-established and widely used clinical procedure. Endoscopic lumbar interbody fusion has several key advantages over other types of procedures, including that it is minimally invasive, more precise, safer, and provides better visualization. Most surgeons use the transforaminal approach for this surgery. Here, we report the first percutaneous endoscopic posterior lumbar interbody fusion (PEPLIF). Methods: The authors introduce the PEPLIF technique with step by step instructions and describe key procedures in detail. Tips for controlling hemorrhages and avoiding complications during the procedure are presented. The authors also discuss the indications and advantages of applying the PEPLIF technique.Results: PEPLIF is a repeatable lumbar fusion technique.Conclusions: PEPLIF is a feasible and effective technique for the fusion of L4-5 and L5-S1 (especially L5-S1). All procedures are completed in full using endoscopy without replacing the cannula. The endoscopic view is similar to that of an open posterior lumbar interbody fusion, thus reducing the learning curve.
Human breast tumour cells viability effect of African Dioscorea Rotundata Tuber extracts in MCF-7 and MDA-MB231 cell lines
Authors: Odimegwu Joy Ifunanya , Odukoya Olukemi Abiodun , Español Alejandro , Sales Maria Elena 
Published: March 2020

Abstract
Background : Several Dioscorea species are used in Chinese medicine for tumours and Dioscorea species grown in Africa are yet to be tested to the best of knowledge for efficacy as cytotoxic agents. In Nigeria, the greatest producer of edible yams in the world, Dioscorea is the king of plants. Its tubers feeds millions of people and also function as ethnomedicine for diverse diseases. We aim to test the efficacy of edible Dioscorea species grown and consumed in Nigeria on two breast cancer cell lines; MCF-7 and MDA-MB231 derived from a luminal and a triple-negative breast cancer (TNBC) respectively and to confirm safety in non-tumour cells MCF-10A (normal breast cell lines) using a well established cytotoxic compound paclitaxel as a standard. Metastatic breast cancer is a prevalent cause of mortality in women around the world. Breast cancer therapies have greatly advanced in recent years, leading to the statement that cancer is not a death sentence but many patients develop cancer re-occurrence and metastasis and subsequently yield to the disease because of chemoresistance. Methods : Ethanolic extracts of Dioscorea rotundata boiled and raw (DiosB and DiosR) respectively were chemically analysed for the presence of diosgenin using HPLC and their cytotoxic activity was tested on MCF-7, MDA-MB-231 and MCF-10A cells I n vitro by MTT assay.Results : DiosB and DiosR extracts were more effective on MCF-7 than on MDA-MB231 cells showing a higher maximal effect on MCF-7 cells than on MDA-MB231 after 24 h and 48 h treatments (p<0.0001 and p<0.05 respectively). DiosR, if applied at a range between 50-70 g/ml, can be effective to reduce breast tumor cell viability without affecting non tumorigenic MCF-10A cells either at 24 h or at 48 h. African yams contain cytotoxic principles. DiosB showed an IC 50 of 38.83µg/ml while DiosR showed an IC 50 of 41.80µg/ml. Discussion : The results have shown that Dioscorea rotundata tubers could be used effectively to treat breast cancer tumors and this is in sync with its diosgenin content, a known cytotoxic compound and the use of other Dioscorea species for similar treatments in Asia etc. A major limitation will be in creating higher demand for the tuber which is consumed as a staple and at the moment affordable to all.Conclusion : There will be need to make consumers aware of the significant anticancer importance of D. rotundat a and a great need to increase its cultivation in order to make it sufficient as food and as medicine.
A differential expression of receptors mediating receptor-mediated transcytosis (RMT) in brain microvessels, brain parenchyma and peripheral tissues of the mouse and the human
Authors: Zhang Wandong , Liu Qing Yan , Haqqani Arsalan S. , Leclerc Sonia , Lei Joy , Fauteux Francois , Baumann Ewa , Delaney Christie E. , Ly Dao , Star Alexandra T. , Brunette Eric , Sodja Caroline , Hewitt Melissa , Sandhu Jagdeep K. , Stanimirovic Danica B. 
Published: March 2020

Abstract
Receptor-mediated transcytosis (RMT) is a principal pathway for transport of macromolecules essential for brain function across the blood-brain barrier (BBB). Antibodies or peptide ligands which bind RMT receptors are often co-opted for brain delivery of biotherapeutics. A constitutively recycling transferrin receptor (TfR) is a prototype receptor utilized to shuttle therapeutic cargos across the BBB. Several other BBB-expressed receptors have been shown to mediate transcytosis of ligands including insulin receptor (INSR) and insulin-like growth factor-1 receptor (IGF1R), lipid transporters LRP1, LDLR, LRP8 and TMEM30A, solute carrier family transporter LAT1 (subunit CD98hc) and leptin receptor (LEPR). In this study, we analyzed expression patterns of genes encoding RMT receptors in isolated brain microvessels, the brain and peripheral organs of the mouse and the human using RNAseq approach. IGF1R, INSR and LRP8 were highly enriched in the mouse brain microvessels compared to peripheral tissues. In the human brain microvessels only INSR was enriched compared to either the brain or the lung. The expression levels of LRP1, IGF1R, LRP8 and TFRC were significantly higher in the mouse compared to the human brain microvessels. The protein expression of these receptors analyzed by quantitative Western blot and immunofluorescent staining of the brain microvessels correlated with their transcript abundance. This study provides a molecular transcriptomics map of key RMT receptors in mouse and human brain microvessels and peripheral tissues, important sot translational studies of biodistribution, efficacy and safety of antibodies developed against these receptors.