bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression profile of SYNE3 and bioinformatic analysis of its prognostic value and functions in tumors
Authors: Liao Liwei , Zhang Longshan , Yang Mi , Wang Xiaoqing , Huang Weiqiang , Wu Xixi , Pan Hua , Yuan Lu , Huang Wenqi , Wu Yuting , Guan Jian 
Published: March 2020

Abstract
Background: Spectrin repeat containing nuclear envelope family member 3 (SYNE3) encodes an important component of linker of cytoskeleton and nucleoskeleton (LINC) complex, namely nesprin-3. In tumor, invasiveness and metastasis rely on the integrity of LINC complex, while the role of SYNE3/nesprin-3 in cancer is rarely studied. Methods: Here, we explored the expression pattern, prognostic value and related mechanisms of SYNE3 through both experimental and bioinformatic methods. We first detected SYNE3 in BALB/c mice, normal human tissues and the paired tumor tissues, then used bioinformatic databases to verify our results. We further analyzed the prognostic value of SYNE3. Next, we predicted miRNA targeting SYNE3 and built a competing endogenous RNA (ceRNA) network and a transcriptional network by analyzing data from the cancer genome atlas (TCGA) database. Interacting genes of SYNE3 were predicted, and we further performed GO and KEGG enrichment analysis on these genes. Besides, the relationship of SYNE3 and immune infiltration was also investigated. Results: SYNE3 exhibited various expressions in different tissues, mainly located on nuclear and in cytoplasm sometimes. SYNE3 expression level had prognostic value in tumors, possibly by stablizing nucleus, promoting tumor cells apoptosis and altering tumor microenvironment. Additionally, we constructed a RP11-2B6.2-miR-149-5p-/LINC01094-miR-330-3p-SYNE3 ceRNA network and a SATB1-miR-149-5p-SYNE3 transcriptional network in lung squamous cell carcinoma to support the tumor-suppressing role of SYNE3. Conclusions: Our study explored novel anti-tumor functions and mechanisms of SYNE3, which might be useful for future cancer therapy.
Prognostic role and treatment tips of FHL1 expression in cytogenetically intermediate- and poor-risk acute myeloid leukemia
Authors: yin chengliang , Liu Yuchen , Chen Qi , Zhao Min , Wu Jing , Sun Jinxiu , Xue Wanguo , Dou Liping , Shi Jinlong 
Published: March 2020

Abstract
Background: Acute myeloid leukemia (AML) is a heterogeneous disorder of hematopoietic system, 35 to 40% of younger patients (<60 years) are recurrent, refractory or drug-resistant, which are canonically called as cytogenetically intermediate- and poor-risk AML (IP-AML). Identifying novel biomarkers is an urgent clinical need and research hotspot for treatment optimization. Methods: Bioinformatics analysis were carried out for RNA-Seq data derived from drug resistance-associated cell lines and The Cancer Genome Atlas (TCGA). Varieties of comparison, visualization and functional enrichments were performed to Four-and-a-half LIM domain 1 (FHL1) and related genes. FHL1's expression changes were confirmed by ‘wet’ experiments. Kaplan-Meier method, log-rank test and multivariate Cox proportional hazards models were utilized to evaluate the associations between FHL1 expression and Overall Survival (OS), Event Free Survival (EFS), Relapse Free Survival (RFS). Prognostic significance of FHL1 expression was further validated in another independent larger IP-AML cohort (GSE6891). Moreover, construction of nomogram and validation of prognostic model. Results: High expression of FHL1 (FHL1 high ) was a potentially effective biomarker of poor prognosis for IP-AML. Compared to FHL1 low group, FHL1 high was associated with short OS and EFS (145 patients, OS, P < 0.001; EFS, P < 0.001), which was further validated in GSE6891 (284 patients, OS, P < 0.001; EFS, P < 0.001). Multivariate analysis also confirmed the adverse prognosis of FHL1 high (HR = 2.2339, P = 0.000156). ROC indicated an ideal predictive accuracy of the outcome model (AUC was 0.773). In addition, to understand the inherent mechanisms FHL1 involved, genome-wide characteristics were investigated to find that FHL1 might be involved in several important carcinogenic signal pathways. Conclusions: FHL1’s expression may serve as an effective prognostic biomarker, and could provide valuable treatment indications for IP-AML patients, whose distinctive gene patterns were helpful to further understand the recurrent, refractory and drug-resistant mechanisms for IP-AML.
Distal Chevron Osteotomy vs The Simple, Effective, Rapid, Inexpensive Technique (SERI) for Mild to Moderate Isolated Hallux Valgus: A Randomized Controlled Study
Authors: Palmanovich Ezequiel , contribution Nissiom Ohana* - equal first author , Small Ilan , Hetsroni Iftach , Amar Eyal , Sharfman Zachary T , Segal David , Atzmon Ran 
Published: March 2020

Abstract
Background Hallux valgus is a common foot deformity that leads to functional disability with serious sequelae. Minimally invasive surgery is often used to treat hallux valgus in order to reduce wound complications and improve recovery time. The objective of this study was to compare a Simple, Effective, Rapid, Inexpensive (SERI) technique with a simple Chevron technique in patients with minimum of one-year follow. Methods and Materials Between the years 2014-2015 we performed a prospective study comparing the SERI minimally invasive technique to treat symptomatic hallux valgus with a standard chevron osteotomy technique. All procedures were performed by a single fellowship trained foot and ankle surgeon. Twenty-one patients were randomized to the SERI cohort and 15 to the standard Chevron technique. Results The mean pre-operative intermetatarsal angle (IMA) of the SERI group was 14.8 ± 1.9 (11.9 - 22.9). The mean pre-operative IMA of the Chevron control group was 13.3 ± 2.3 (10.4 -18.2) (p = 0.038). The mean IMA two weeks after surgery was 6.0 ± 2.3 (2.4-12) in the SERI group, and 6.1 ± 3 (2.6-13.1) in the control group. At the two week and one year follow up, there was no significant difference found in the IMA between the two groups (p = 0.871). Neither groups reported symptomatic transfer metatarsalgia throughout the follow up period. The SERI group had increased metatarsophalangeal joint (MTPJ) motion (p < 0.001) however, all other parameters with similar. Conclusion The SERI technique provided comparable outcomes at up to one year follow up when compared to a standard Chevron osteotomy for moderate hallux valgus. This study demonstrated good reproducible results using the SERI technique for moderate hallux valgus.
Incidence of Uveitis and Macular Edema among Patients taking Fingolimod 0.5mg for Multiple Sclerosis
Authors: Sonne Scott Joseph , Smith Bradley Thomas 
Published: March 2020

Abstract
Background Patients with multiple sclerosis (MS) have a higher incidence of uveitis compared with the general population. Fingolimod, a first line disease modifying drug used in multiple sclerosis, may cause macular edema and thus requires ophthalmic examination. However, murine models and anecdotal reports suggest fingolimod may reduce the incidence of uveitis. Purpose To report the incidence of uveitis and macular edema among those on fingolimod 0.5 mg (Gilenya®) therapy for Multiple Sclerosis (MS). Methods Retrospective review of patients on fingolimod who developed uveitis and/or macular edema. Results No patients had an occurrence or history of uveitis. Four of the 188 (2.13%) patients developed macular edema without ocular inflammation. One of the 188 (0.53%) patients developed Acute Macular Neuroretinopathy. Conclusion Patients taking fingolimod have a lower incidence of uveitis than expected in a population of MS patients.
Experiential learning for children's dental anxiety: a cluster randomized trial
Authors: Zhu Minmin , Yu Hongbing , Xie Bo , Li Hongwen , He Qian , Li Huimin , Su Jing , Li Xueqi 
Published: March 2020

Abstract
Background Dental anxiety (DA) has an impact on the quality of dental treatment and may have long-lasting implications for children. A recent study introducing experiential learning (EL) into children's oral health promotion resulted in better oral hygiene. The purpose of the study is to evaluate whether EL can reduce children's DA. Methods In September 2018, we recruited 988 children aged 7-8 years from 24 classes to participate in a cluster-randomized trial. Classes were randomly assigned to EL (in which children received a lively presentation on oral health and participated in a role play in a simulated dental clinic in the classroom) or the Tell-Show-Do (TSD) group (in which children received a conventional TSD behavior management). The primary outcome was the prevalence of DA after the procedure of pit and fissure sealant (PFS), assessed by a modified children's fear survey schedule-dental subscale. Secondary outcomes were changes in blood pressures (BP) and pulse rates (PR) before and after the PFS procedure. The intervention effects were estimated by means of mixed effect models, which included covariates of gender and school (and baseline value for BP and PR only), and a random cluster effect. Results In 396 children of the EL group who received the PFS treatment, the prevalence of DA (score > 38) was 18.5%, compared with 24.3% in 391 children of the TSD group (OR=0.65; 95% confidence interval, 0.46-0.93; P =0.019). The increases in BP and PR after the PFS were also significantly less in the EL group. Conclusions School-based experiential learning intervention before a dental visit is feasible and effective in lowering children's dental anxiety.
Re-evaluation of Nucleic Acid Retested Positive Cases in the Recovered COVID-19 Patients: Report from a Designated Transfer Hospital in Chongqing, China
Authors: Chen Yan , Bai Weizhi , Liu Bin , Huang Jian , Laurent Irakoze , Deng Wuquan , Xiao Xiaoqiu 
Published: March 2020

Abstract
Since the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, Hubei province, China, 1 the epidemic has spread rapidly in Hubei province and other regions in China. A total of 80,552 patients confirmed with COVID-19 have been reported in the main land China up to March 5th, 2020. 2 They included a huge number of patients discharged from hospital. A total of 53726 cases have met the discharge criteria (one of the criteria includes two continued negative result of nucleic acid test with repeated interval period of at least 1day or 24 hours.) in mainland China up to March 5th, 2020. Previous studies have paid more attention to the epidemic situation of COVID-19 and patient's diagnosis and treatment.Closely attention should be paid to the discharged patients. Surprising, previous follow-up reported that some patients nucleic acid retest result was positive again after discharge. 3 Impact factors should be further investigated. Since the first confirmed case was diagnosed in our hospital (Chongqing Emergency Medical Center, the designated transfer hospital) on February 4th, we have confirmed a total of 17 cases. All the patients infected with the novel coronavirus have been transferred to a designated hospital in Southwest China's Chongqing by ambulance with an inbuilt negative-pressure chamber. 4In the follow-up of these patients, all patients accepted RT-PCR tests again after having discharged from designated hospital 3 days later. Four of them showed recurrence of positive results after few days of discharge. Thus, we reported these cases aiming to provide information on policy formulation and modification of discharge plans.
The removal efficiency of iron and manganese from pre-ozonated groundwater using limestone filter
Authors: Abdul Aziz Hamidi , Shakr Shahanis Nabila Mohd , Akbar Nor Azliza , Alazaiza Motasem Y. D. 
Published: March 2020

AbstractThis paper investigates the suitability of limestone to attenuate Fe and Mn from pre-ozonated groundwater samples using batch study and fixed column bed. The effects of limestone dosage, shaking speed, contact time, and pH on the removal efficiency of Fe and Mn are discussed. Pre-ozonated groundwater samples were prepared to present actual samples from Pintu Geng water treatment plant. A general characterization for groundwater as well as for limestone was conducted. In addition, the breakthrough and exhaustion points for limestone were determined. Results showed that limestone has good potential to remove more than 90% of Fe and Mn at optimum dosage of 40 g. The removal percentage for both metals was also affected by changing the contact time, where the maximum removal of Fe and Mn was observed at 90 and 120 minutes, respectively, at pH 8 and shaking speed of 350 rpm. The breakthrough time and exhaustion time of Fe were 40 hours and 210 hours, respectively. However, faster breakthrough time (30 minutes) and exhaustion time (16 hours) were observed for Mn sorption. The findings showed that an integrated ozone-limestone adsorption process significantly enhanced the removal of Fe and Mn up to 99.5% and 92%, respectively.
Binding Mode Prediction and Identification of New Lead Compounds from Natural Products as 3-OST Enzyme Inhibitors
Authors: Sousa Rui , Moorthy Narayana Subbiah Hari Narayana , Fernandes Pedro Alexandrino , Ramos Maria Joao , Brás Natércia Fernandes 
Published: March 2020

Background and Introduction:
The availability of antiviral medicines for the treatment
of viral diseases is limited, hence the discovery of novel bioactive molecules is required. The present
investigation has been carried out to develop novel 3-O-sulfotransferase enzyme inhibitors to
treat viral diseases.

Method:
Virtual screening study (QSAR, docking and pharmacophore analysis) and binding mode
analysis have been performed on a dataset collected from the literature (synthetic and natural compounds).

Results:
The docking studies showed that Glu184, His186, Lys215 and Lys368 residues
established the most important hydrogen bonding with several hit compounds. The QSAR results
explained that the presence of electronegative atoms/groups in the aromatic or heteroaromatic
rings confer increased activity. Furthermore, the flexibility and the aromatic rings with less polar
groups have better activity than the compounds connected to purine rings. Finally, the structurebased
pharmacophore studies illustrated that the ligand has many polar interaction sites, and the projected
acceptor and donor groups in the molecules make a significant contribution to the pharmacophore
model building.

Conclusion:
These studies identified two compounds, Phomoidride B and Barceloneic acid A, as
potential 3-OST inhibitors.
Investigation on the Solidification Behavior of Bi Droplets Embedded in Cu Matrix by Nanocalorimetry
Authors: Wu Mannan , Zhao Bingge , Ding Kai , Ma Hailan , Wu Guanzhi , Zhai Qijie , Gao Yulai 
Published: March 2020

Background:
The solidification of metals is usually initiated by heterogeneous nucleation,
which, however, is less controllable due to the unexpected impurities. In this study, the nucleation
behavior of micro- and nano-sized Bi droplets embedded in the Cu matrix, where well-identified
catalyst interfaces were introduced, was studied.

Objective:
This paper demonstrated the nucleation behavior of the nano-sized Bi droplets embedded
in the Cu matrix to determine the nucleation sites and contact angle.

Methods:
Cu-20Bi (wt. %) melt-spun ribbons consisting of numerous micro- and nano-sized Bi
droplets embedded in the Cu matrix were prepared. Based on the structure characterization by transmission
electron microscopy (TEM), the nucleation behavior of these embedded Bi droplets was
demonstrated by nanocalorimetry.

Results:
The orientation relationship between the nano-sized Bi droplets and the Cu matrix was determined.
The undercooling of the nano-sized Bi droplets with the cooling rate was more stable than
that of the micro-sized Bi droplets, and a nucleation contact angle of 72o was obtained.

Conclusion:
The undercooling increases with the reduction of droplet size. The nucleation of the Bi
droplets is significantly affected by their interfacial structure with the Cu matrix. Compared with
hexagonal Zn, the Cu matrix with face-centered cubic structure has a less catalytic effect on the nucleation
of Bi.
Gadolinium Oxide Nanoparticles Enhance the Cytotoxicity of Chemotherapeutic Drugs by Blocking Autophagic Flux in Human Ovarian Cancer Cells
Authors: Zhang Tianyu , Zhong Cheng , Xie Zhixiong 
Published: March 2020

Background:During chemotherapy, drugs can damage cancer cells’ DNA and cytomembrane structure, and then induce cell death. However, autophagy can increase the chemotherapy resistance of cancer cells, reducing the effect of chemotherapy.Objective:To block the autophagic flux in cancer cells, it is vital to enhance the anti-cancer efficacy of chemotherapy drugs; for this purpose, we test the gadolinium oxide nanoparticles (Gd2O3NPs)’ effect on autophagy.Methods:The cytotoxicity of Gd2O3NPs on HeLa cells was evaluated by a (4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide assay. Then, monodasylcadaverine staining, immunofluorescence, immunoblot, and apoptosis assay were conducted to evaluate the effect of Gd2O3NPs on autophagy and efficacy of chemotherapy drugs in human ovarian cancer cells.Results:We found that Gd2O3NPs, which have great potential for use as a contrast agent in magnetic resonance imaging, could block the late stage of autophagic flux in a dose-dependent manner and then cause autophagosome accumulation in HeLa cells. When co-treated with 8 μg/mL Gd2O3NPs and 5 μg/mL cisplatin, the number of dead HeLa cells increased by about 20% compared with cisplatin alone. We observed the same phenomenon in cisplatin-resistant COC1/DDP cells.Conclusion:We conclude that Gd2O3NPs can block the late stage of autophagic flux and enhance the cytotoxicity of chemotherapeutic drugs in human ovarian cancer cells. Thus, the nanoparticles have significant potential for use in both diagnosis and therapy of solid tumor.
Tuning the Mechanical and Dielectric Properties of Zinc Incorporated Hydroxyapatite
Authors: Qamar Alliya , Zia Rehana , Raiz Madeeha 
Published: March 2020

Background:Hydroxyapatite is similar to bone mineral in chemical composition and has good biocompatibility with host tissue and bone.Objective:This work aims to tailor the mechanical and dielectric properties of hydroxyapatite with zinc substitution, to improve the wearability of implant and accelerate the healing process.Methods:Pure and zinc incorporated hydroxyapatite Ca10(PO4)6(OH)2 samples have been successfully prepared by means of the chemical precipitation method.Results:The results showed that hydroxyapatite(Hap) having a hexagonal structure was the major phase identified in all the samples. It was found that the secondary phase of β-tricalcium phosphate (β-TCP) formed due to the addition of zinc, resulting in biphasic structure BCP (Hap + β-TCP). A minor phase of ZnO also formed for a higher concentration of Zn (Zn ≥ 2mol%) doping. It was found that the Zn incorporation to Hap enhanced both mechanical and dielectric properties without altering the bioactive properties. The microhardness increased up to 0.87 GPa for Zn concentration equal to 1.5mol%, which is comparable to the human bone ~0.3 - 0.9 GPa. The dielectric properties evaluated in the study showed that 1.5 mol% Zn doped hydroxyapatite had the highest dielectric constant. Higher values of dielectric constant at low frequencies signify its importance in healing processes and bone growth due to polarization of the material under the influence of the electric field.Conclusion:Sample Z1.5, having 1.5 mol% Zn doping, showed the most optimized properties suitable for bone regeneration applications.
Differential Gene Expression of ASUN, NEMF, PTPRC and DHX29: Candidate Biomarkers for the Diagnosis of Active and Latent Tuberculosis
Authors: Mamishi Setareh , Pourakbari Babak , Sadeghi Reihaneh Hosseinpour , Marjani Majid , Mahmoudi Shima 
Published: March 2020

Introduction:
Tuberculosis (TB) remains one of the most important infectious causes of
death throughout the world. A wide range of technologies have been used for the diagnosis of TB.
However, current diagnostic tests are inadequate. The aim of this study was to evaluate the expression
of four genes, namely ASUN, NEMF, PTPRC and DHX29 as candidate biomarkers for the diagnosis
of Latent tuberculosis infection (LTBI) and active TB and discrimination of active TB and
LTBI.

Material and Methods:
The expression of the mentioned four genes as well as ACTB as a housekeeping
gene was evaluated by real-time PCR. Receiver operating characteristic (ROC) curve analysis
was conducted to assess the specificity and sensitivity of each validated biomarker.

Results:
Our results showed that the expression of theASUN gene could discriminate between active
TB cases and healthy BCG vaccinated volunteers with an AUC value of 0.76, combing with a
sensitivity of 68% and a specificity of 67%. It should be noted that the PTPRC gene also has the potential
for the diagnosis of active TB with an AUC value of 0.67 and a sensitivity of 64.5% and a
specificity of 70%. The curve revealed that cases with LTBI could be distinguished from healthy
BCG vaccinated volunteers according to their expression of the ASUN gene with an AUC value of
0.81. The cut-off value for diagnosing was 11, with a sensitivity of 73% and a specificity of 79%.
Moreover, the expression of the NEMF gene might be considered as a diagnostic tool for the diagnosis
of LTBI. The analysis showed an AUC value of 0.75. The highest sensitivity (60%) and specificity
(81%) were obtained with a cut off value of 12.

Conclusion:
According to our results, the expression of ASUN and NEMF genes might be considered
as a diagnostic tool for the diagnosis of LTBI. Our study showed that the expression of ASUN
and PTPRC was obviously higher in active TB patients than those in healthy BCG vaccinated controls.
On the other hand, DHX29 and PTPRC genes might be helpful in differentiating active TB
and LTBI. However, our findings deserve further validation in larger studies.
Time-Dependent Effects in Chronic Urticaria: A Time-Series Perspective of Omalizumab Treatment
Authors: Tagka Anna , Lambrou George I. , Makris Michael , Nakou Evangelia , Nicolaidou Electra , Chatziioannou Argyro , Katsarou Alexandra 
Published: March 2020

Background:
Chronic spontaneous urticaria (CSU, or CU) is a disease that significantly
affects the quality of life of patients. It is a chronic disease and requires a specialized approach to
diagnosis and treatment. In recent years, the disease has been of great interest due to the existence of
new targeted therapeutic approaches.

Aims:
The present study aims at analyzing CU score concerning time, as a time-series. The authors have
attempted to model the investigated time-series to unravel possible causative relationships.

Methods:
108 patients (25Males/83Females) admitted to our department were diagnosed with CU. CU
was estimated on a score basis, which was used to define disease severity. Urticaria score was assessed
on the basis of Urticaria Activity Score 7 (UAS7). The mean CU score, the mean CU score rate concerning
the first month at diagnosis as well as the monthly CU score rate were calculated.

Result:
Gender is a factor that influences CU score with time. In addition, there was a significant finding
that time-series differ with the administration of monotherapy or complementary therapy.

Conclusion:
We have found that females are more prone to CU, while omalizumab monotherapy has
more beneficial results as compared to the application of concurrent and maintenance therapies. Further,
patients with co-morbidities were more likely to interrupt treatment. Finally, and most significantly, it
was shown that monthly CU score rate manifested an oscillatory pattern, which was modelled with the
sum of sines functions, highlighting a relative immunological pattern.