bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adherence with brand versus generic bisphosphonates among osteoporosis patients: a new-user cohort study in the French National Healthcare Insurance database
Authors: Viprey Marie , Xue Yufeng , Rousseau Aurélie , Payet Cécile , Chapurlat Roland , Caillet Pascal , Dima Alexandra , Schott Anne-Marie 
Published: May 2020

AbstractSeveral studies documented declines in treatment adherence with generic forms of oral bisphosphonates in osteoporosis compared to branded forms, while others did not support this relation. Our aim was to compare medication adherence with brand versus generic forms of oral bisphosphonates. A new-user cohort study was conducted using routinely collected administrative and healthcare data linked at the individual level extracted from a nationwide representative sample of the French National Healthcare Insurance database. We included all patients aged 50 and older, new users of oral bisphosphonates for primary osteoporosis between 01/01/2009 and 31/12/2015. Two components of adherence were measured: implementation (continuous multiple-interval measure of medication availability version 7; CMA7) and persistence (time to discontinuation). The sample was composed of 1,834 in the “brand bisphosphonate” group and 1,495 patients in the “generic bisphosphonate” group. Initiating oral bisphosphonate treatment with brand was associated with a higher risk of discontinuation within 12 months (Hazard Ratio = 1.08; 95%CI = [1.02;1.14]). The risk of good implementation (CMA7 ≥ 0.90) was significantly lower in “brand bisphosphonate” group (Risk Ratio = 0.90; 95%CI = [0.85; 0.95]). We did not find any evidence to support the hypothesis of a lower adherence to generic bisphosphonates. In fact, prescribing of generic bisphosphonates led to a higher persistence rate and to better implementation at 1 year.
Topical rifampicin for prevention of deep sternal wound infections in patients undergoing coronary artery bypass grafting
Authors: De Santo Luca Salvatore , Rubino Antonino Salvatore , Torella Michele , De Feo Marisa , Galgano Viviana , Guarente Nicola , Mango Emilio , Savarese Leonardo , Iorio Francesco , Zebele Carlo 
Published: May 2020

AbstractDeep sternal wound infections (DSWI), although an infrequent complication, significantly impair postoperative outcomes after coronary artery bypass grafting (CABG) surgery. Among several preventive strategies, topical antibiotic therapy immediately before sternal closure has been strongly advocated. In this retrospective analysis, the incidence of DSWI in 517 patients undergoing isolated CABG and receiving rifampicin irrigation of mediastinum, sternum and suprasternal tissues was compared to an historical consecutive cohort of 448 patients. To account for the inherent selection bias, a 1:1 propensity matched analysis was performed. Patients receiving topical rifampicin experienced significantly less occurrence of postoperative DSWI (0.2% vs 2.5%, p = 0.0016 in the unmatched analysis; 0.3% vs 2.1%, p = 0.0391 in the matched analysis). Intensive care unit stay, hospital stay, and operative mortality were similar between groups. This study shows that topical rifampicin in combination with commonly prescribed preventative strategies significantly reduces the incidence of DSWI to less than 0.3% in unselected patients undergoing a full median sternotomy for CABG. Further studies, including a larger number of patients and with a randomization design, would establish the potential preventative role of topical rifampicin in reducing the occurrence of DSWI.
The Jurassic magmatism of the Demerara Plateau (offshore French Guiana) as a remnant of the Sierra Leone hotspot during the Atlantic rifting
Authors: Basile Christophe , Girault Igor , Paquette Jean-Louis , Agranier Arnaud , Loncke Lies , Heuret Arnauld , Poetisi Ewald 
Published: May 2020

AbstractWe report the discovery of 173.4 Ma hotspot-related magmatic rocks in the basement of the Demerara Plateau, offshore French Guiana and Suriname. According to plate reconstructions, a single hotspot may be responsible for the magmatic formation of (1) both the Demerara Plateau (between 180 and 170 Ma) and the Guinea Plateau (circa 165 Ma) during the end of the Jurassic rifting of the Central Atlantic; (2) both Sierra Leone and Ceara Rises (mainly from 76 to 68 Ma) during the upper Cretaceous oceanic spreading of the Equatorial Atlantic ocean; (3) the Bathymetrists seamount chain since the upper Cretaceous. The present-day location of the inferred Sierra Leone hotspot should be 100 km west of the Knipovich Seamount.
Ecosystem state change in the Arabian Sea fuelled by the recent loss of snow over the Himalayan-Tibetan Plateau region
Authors: Goes Joaquim I. , Tian Hongzhen , Gomes Helga do Rosario , Anderson O. Roger , Al-Hashmi Khalid , deRada Sergio , Luo Hao , Al-Kharusi Lubna , Al-Azri Adnan , Martinson Douglas G. 
Published: May 2020

AbstractThe recent trend of global warming has exerted a disproportionately strong influence on the Eurasian land surface, causing a steady decline in snow cover extent over the Himalayan-Tibetan Plateau region. Here we show that this loss of snow is undermining winter convective mixing and causing stratification of the upper layer of the Arabian Sea at a much faster rate than predicted by global climate models. Over the past four decades, the Arabian Sea has also experienced a profound loss of inorganic nitrate. In all probability, this is due to increased denitrification caused by the expansion of the permanent oxygen minimum zone and consequent changes in nutrient stoichiometries. These exceptional changes appear to be creating a niche particularly favorable to the mixotroph, Noctiluca scintillans which has recently replaced diatoms as the dominant winter, bloom forming organism. Although Noctiluca blooms are non-toxic, they can cause fish mortality by exacerbating oxygen deficiency and ammonification of seawater. As a consequence, their continued range expansion represents a significant and growing threat for regional fisheries and the welfare of coastal populations dependent on the Arabian Sea for sustenance.
Analysis of corneal real astigmatism and high order aberration changes that cause visual disturbances after lower eyelid epiblepharon repair surgery
Authors: Lee Dong Cheol 
Published: May 2020

AbstractThis retrospective, cross-sectional study investigated changes in corneal low and high order aberrations (LOAs and HOAs) after lower eyelid epiblepharon repair surgery. In total, 108 eyes from 54 patients were evaluated. Wavefront analyses for calibrated LOAs and HOAs were performed using a Galilei G4 Dual Scheimpflug Analyzer before surgery and during the first and second follow-ups (f/u), adjusting for several risk factors. Flat keratometry (K) and axis values decreased significantly from baseline at the first f/u. At the second f/u, mean K and axis values decreased. Coma and trefoil increased from baseline at the first f/u and normalized by the second f/u. Spherical aberrations (SA) only decreased at the second f/u. After correction for risk factors, cylinder, coma, trefoil, and SA significantly increased at the first f/u; axis and flat K values decreased. At the second f/u, cylinder increased while axis and mean K values significantly decreased. Epiblepharon repair surgery may result in a shift from ‘with-the-rule’ to ‘against-the-rule’ axis change. Flat K, coma, and trefoil may be affected by mechanical force changes immediately post-surgery, while mean K values and SA may also change with corneal state changes including corneal erosion healing after the second f/u during the postoperative period.
Challenges to rutile-based geoscientific tools: low-temperature polymorphic TiO2 transformations and corresponding reactive pathways
Authors: Pinto André Jorge , Sanchez-Pastor Nuria , Callegari Ivan , Pracejus Bernhard , Scharf Andreas 
Published: May 2020

AbstractRutile, a common accessory mineral in a wide variety of rocks, is the most stable naturally occurring TiO2 polymorph. The relationship between its trace element composition and formation conditions has provided geoscientists with discriminant tools for fingerprinting geological processes, such as magmatic evolution and subduction zone metamorphism, alongside applications to the study of sediment provenance. In the present work, volcaniclastic rock samples belonging to Fara and Saiq Formations, outcropping in Jebel Akhdar mountains, Oman, are studied with Raman spectroscopy and Electron Microprobe (EMP) aiming: of (i) the identification of different naturally-occurring TiO2 polymorphs, (ii) the evaluation of their trace element contents in relation with hydrothermal alteration features, and (iii) the analysis of the mineral reactive pathways behind the observed textural relationships. Raman investigations demonstrated that interstitial, fine-grained TiO2 corresponds to anatase, whereas rutile occurs as isolated single grains. EMP determinations further revealed that an identified Nb-enrichment in anatase is coupled with a corresponding Nb-depletion in rutile. The combination of the obtained results with petrographic observations enabled unravelling the TiO2 reactive pathways affecting the studied samples. Thus, a coupled polymorphic dissolution-precipitation reaction assisting rutile-to-anatase conversion has been defined, together with the role of Nb in further stabilizing the structure of the lower temperature polymorph. Semi-quantitative thermometric considerations suggest that rutile substrates are likely of magmatic origin, whereas anatase formation is clearly associated with a lower temperature aqueous environment. The gathered results raise fundamental questions concerning the application of commonly used rutile-based geochemical and thermometric tools.
Electronegativity and doping in Si1-xGex alloys
Authors: Christopoulos Stavros-Richard G. , Kuganathan Navaratnarajah , Chroneos Alexander 
Published: May 2020

AbstractSilicon germanium alloys are technologically important in microelectronics but also they are an important paradigm and model system to study the intricacies of the defect processes on random alloys. The key in semiconductors is that dopants and defects can tune their electronic properties and although their impact is well established in elemental semiconductors such as silicon they are not well characterized in random semiconductor alloys such as silicon germanium. In particular the impact of electronegativity of the local environment on the electronic properties of the dopant atom needs to be clarified. Here we employ density functional theory in conjunction with special quasirandom structures model to show that the Bader charge of the dopant atoms is strongly dependent upon the nearest neighbor environment. This in turn implies that the dopants will behave differently is silicon-rich and germanium-rich regions of the silicon germanium alloy.
Inhibitory action of phenothiazinium dyes against Neospora caninum
Authors: Pereira Luiz Miguel , Mota Caroline Martins , Baroni Luciana , Bronzon da Costa Cássia Mariana , Brochi Jade Cabestre Venancio , Wainwright Mark , Mineo Tiago Wilson Patriarca , Braga Gilberto Úbida Leite , Yatsuda Ana Patrícia 
Published: May 2020

AbstractNeospora caninum is an Apicomplexan parasite related to important losses in livestock, causing abortions and decreased fertility in affected cows. Several chemotherapeutic strategies have been developed for disease control; however, no commercial treatment is available. Among the candidate drugs against neosporosis, phenothiazinium dyes, offer a low cost-efficient approach to parasite control. We report the anti-parasitic effects of the phenothiaziums Methylene Blue (MB), New Methylene Blue (NMB), 1,9–Dimethyl Methylene Blue (DMMB) and Toluidine Blue O (TBO) on N. caninum, using in vitro and in vivo models. The dyes inhibited parasite proliferation at nanomolar concentrations (0.019–1.83 μM) and a synergistic effect was achieved when Methylene Blue was combined with New Methylene Blue (Combination Index = 0.84). Moreover, the phenothiazinium dyes improved parasite clearance when combined with Pyrimethamine (Pyr). Combination of Methylene Blue + 1,9–Dimethyl Methylene Blue demonstrated superior efficacy compared to Pyrimethamine based counterparts in an in vivo model of infection. We also observed that Methylene Blue, New Methylene Blue and 1,9–Dimethyl Methylene Blue increased by 5000% the reactive oxygen species (ROS) levels in N. caninum tachyzoites. Phenothiazinium dyes represent an accessible group of candidates with the potential to compound future formulations for neosporosis control.
Integrative analysis of Mendelian randomization and Bayesian colocalization highlights four genes with putative BMI-mediated causal pathways to diabetes
Authors: Liu Qian , Pan Jianxin , Berzuini Carlo , Rutter Martin K. , Guo Hui 
Published: May 2020

AbstractGenome-wide association studies have identified hundreds of single nucleotide polymorphisms (SNPs) that are associated with BMI and diabetes. However, lack of adequate data has for long time prevented investigations on the pathogenesis of diabetes where BMI was a mediator of the genetic causal effects on this disease. Of our particular interest is the underlying causal mechanisms of diabetes. We leveraged the summary statistics reported in two studies: UK Biobank (N = 336,473) and Genetic Investigation of ANthropometric Traits (GIANT, N = 339,224) to investigate BMI-mediated genetic causal pathways to diabetes. We first estimated the causal effect of BMI on diabetes by using four Mendelian randomization methods, where a total of 76 independent BMI-associated SNPs (R2 ≤ 0.001, P < 5 × 10−8) were used as instrumental variables. It was consistently shown that higher level of BMI (kg/m2) led to increased risk of diabetes. We then applied two Bayesian colocalization methods and identified shared causal SNPs of BMI and diabetes in genes TFAP2B, TCF7L2, FTO and ZC3H4. This study utilized integrative analysis of Mendelian randomization and colocalization to uncover causal relationships between genetic variants, BMI and diabetes. It highlighted putative causal pathways to diabetes mediated by BMI for four genes.
DNA methylation and gene expression signatures are associated with ataxia-telangiectasia phenotype
Authors: McGrath-Morrow Sharon A. , Ndeh Roland , Helmin Kathryn A. , Khuder Basil , Rothblum-Oviatt Cynthia , Collaco Joseph M. , Wright Jennifer , Reyfman Paul A. , Lederman Howard M. , Singer Benjamin D. 
Published: May 2020

AbstractPeople with ataxia-telangiectasia (A-T) display phenotypic variability with regard to progression of immunodeficiency, sino-pulmonary disease, and neurologic decline. To determine the association between differential gene expression, epigenetic state, and phenotypic variation among people with A-T, we performed transcriptional and genome-wide DNA methylation profiling in patients with mild and classic A-T progression as well as healthy controls. RNA and genomic DNA were isolated from peripheral blood mononuclear cells for transcriptional and DNA methylation profiling with RNA-sequencing and modified reduced representation bisulfite sequencing, respectively. We identified 555 genes that were differentially expressed among the control, mild A-T, and classic A-T groups. Genome-wide DNA methylation profiling revealed differential promoter methylation inciswith 146 of these differentially expressed genes. Functional enrichment analysis identified significant enrichment in immune, growth, and apoptotic pathways among the methylation-regulated genes. Regardless of clinical phenotype, all A-T participants exhibited downregulation of critical genes involved in B cell function (PAX5,CD79A,CD22, andFCRL1) and upregulation of several genes associated with senescence and malignancy, includingSERPINE1. These findings indicate that gene expression differences may be associated with phenotypic variability and suggest that DNA methylation regulates expression of critical immune response genes in people with A-T.
Super-resolution in situ analysis of active ribosomal DNA chromatin organization in the nucleolus
Authors: Maiser Andreas , Dillinger Stefan , Längst Gernot , Schermelleh Lothar , Leonhardt Heinrich , Németh Attila 
Published: May 2020

AbstractRibosomal RNA (rRNA) transcription by RNA polymerase I (Pol I) is the first key step of ribosome biogenesis. While the molecular mechanisms of rRNA transcription regulation have been elucidated in great detail, the functional organization of the multicopy rRNA gene clusters (rDNA) in the nucleolus is less well understood. Here we apply super-resolution 3D structured illumination microscopy (3D-SIM) to investigate the spatial organization of transcriptionally competent active rDNA chromatin at size scales well below the diffraction limit by optical microscopy. We identify active rDNA chromatin units exhibiting uniformly ring-shaped conformations with diameters of ~240 nm in mouse and ~170 nm in human fibroblasts, consistent with rDNA looping. The active rDNA chromatin units are clearly separated from each other and from the surrounding areas of rRNA processing. Simultaneous imaging of all active genes bound by Pol I and the architectural chromatin protein Upstream Binding Transcription Factor (UBF) reveals a random spatial orientation of regular repeats of rDNA coding sequences within the nucleoli. These observations imply rDNA looping and exclude potential formation of systematic spatial assemblies of the well-ordered repetitive arrays of transcription units. Collectively, this study uncovers key features of the 3D organization of active rDNA chromatin units and their nucleolar clusters providing a spatial framework of nucleolar chromatin organization at unprecedented detail.
Genetic basis of sleep bruxism and sleep apnea—response to a medical puzzle
Authors: Wieckiewicz Mieszko , Bogunia-Kubik Katarzyna , Mazur Grzegorz , Danel Dariusz , Smardz Joanna , Wojakowska Anna , Poreba Rafal , Dratwa Marta , Chaszczewska-Markowska Monika , Winocur Efraim , Emodi-Perlman Alona , Martynowicz Helena 
Published: May 2020

AbstractSleep bruxism (SB) and obstructive sleep apnea (OSA) are co-occurring sleep conditions. The study aimed to evaluate the association of selected single-nucleotide polymorphisms (SNPs) occurring within the genes of the serotonin and dopamine pathways in SB and OSA and investigate the relationship between them. The study group included 100 Caucasian patients. SB and OSA were diagnosed in 74 and 28 patients, respectively. In addition, 125 unrelated Caucasian healthy blood donors served as randomly selected controls to enable comparison of polymorphisms. The following SNPs were analyzed: rs2770304 and rs6313 within the serotonin receptor encoding gene (HTR2A), rs4680 polymorphism of the catechol-O-methyltransferase (COMT) gene, and rs686 within the dopamine receptor (DRD1) encoding gene. The prevalence of the DRD1 rs686 G variant (GG homozygosity) was found to be high in the study group compared to the control group. Bruxism episode index (BEI) was found to be significantly increased in the HTR2A rs6313 TT homozygotes compared to the heterozygous patients. Moreover, within a group of the HTR2A rs2770304 TT homozygous cases, a statistically significant correlation was observed between BEI and apnea–hypopnea index. These results indicate that DRD1 rs686 may potentially affect predisposition to SB, that HTR2A rs6313 SNP may be involved in SB pathogenesis, and that HTR2A rs2770304 polymorphism might contribute to the association between SB and OSA. This suggests a possible genetic contribution to the etiology of primary SB.
miR-618 rs2682818 C&gt;A polymorphism decreases Hirschsprung disease risk in Chinese children
Authors: Zheng Yi , Lu Tongyi , Xie Xiaoli , He Qiuming , Lu Lifeng , Zhong Wei 
Published: May 2020

Abstract
MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play an important role in the development of many malignant tumors. In addition, recent studies have reported that single nucleotide polymorphisms (SNPs) located in the miRNA functional region was inextricably linked to tumor susceptibility. In the present study, we investigated the susceptibility between miR-618 rs2682818 C&gt;A and Hirschsprung disease (HSCR) in the Southern Chinese population (1470 patients and 1473 controls). Odds ratios (ORs) and 95% confidence intervals (CIs) were used for estimating the strength of interrelation between them. We found that the CA/AA genotypes of miR-618 rs2682818 were associated with a decreased risk of HSCR when compared with the CC genotype (OR = 0.84, 95% CI = 0.72–0.99, P=0.032). Based on the stratified analysis of HSCR subtypes, the rs2682818 CA/AA genotypes were able to significantly lessen the risk of HSCR compared with CC genotype in patients with long-segment HSCR (adjusted OR = 0.70, 95% CI = 0.52–0.93, P=0.013). In conclusion, our results indicated that the miR-618 rs2682818 C&gt;A polymorphism was associated with a reduced risk of HSCR in Chinese children, especially in patients with long-segment HSCR (L-HSCR) subtype.
Full 5D characterisation of the Sagittarius stream with Gaia DR2 RR Lyrae
Authors: Ramos P. , Mateu C. , Antoja T. , Helmi A. , Castro-Ginard A. , Balbinot E. , Carrasco J. M. 
Published: May 2020

Context. The Sagittarius (Sgr) stream is one of the best tools that we currently have to estimate the mass and shape of our Galaxy. However, assigning membership and obtaining the phase-space distribution of the stars that form the tails of the stream is quite challenging.
Aims. Our goal is to produce a catalogue of the RR Lyrae stars of Sgr and obtain an empiric measurement of the trends along the stream in sky position, distance, and tangential velocity.
Methods. We generated two initial samples from the Gaia DR2 RR Lyrae catalogue: one selecting only the stars within ±20° of the orbital plane of Sagittarius (Strip), and the other resulting from application of the Pole Count Map (nGC3) algorithm. We then used the model-independent, deterministic method developed in this work to remove most of the contamination by detecting and isolating the stream in distance and proper motions.
Results. The output is two empiric catalogues: the Strip sample (higher-completeness, lower-purity) which contains 11 677 stars, and the nGC3 sample (higher-purity, lower-completeness) with 6608 stars. We characterise the changes along the stream in all the available dimensions, namely the five astrometric dimensions plus the metallicity, covering more than 2π rad in the sky, and obtain new estimates for the apocentres and the mean [Fe/H] of the RR Lyrae population. Also, we show the first map of the two components of the tangential velocity thanks to the combination of distances and proper motions. Finally, we detect the bifurcation in the leading arm and report no significant difference between the two branches in terms of metallicity, kinematics, or distance.
Conclusions. We provide the largest sample of RR Lyrae candidates of Sgr, which can be used as input for a spectroscopic follow-up or as a reference for the new generation of models of the stream through the interpolators in distance and velocity that we constructed.