bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patients With Traumatic Brain Injury Transported by Critical Care Air Transport Teams: The Influence of Altitude and Oxygenation during Transport
Authors: Maddry Joseph K , Araña Allyson A , Reeves Lauren K , Mora Alejandra G , Gutierrez Xandria E , Perez Crystal A , Ng Patrick C , Griffiths Sean A , Bebarta Vikhyat S 
Published: June 2020

ABSTRACTIntroductionTraumatic brain injuries (TBIs) are life-threatening, and air transport of patients with TBI requires additional considerations. To mitigate the risks of complications associated with altitude, some patients fly with a cabin altitude restriction (CAR) to limit the altitude at which an aircraft’s cabin is maintained. The goal of this study was to examine the effects of CARs on patients with TBI transported out of theater via Critical Care Air Transport Teams.Materials and MethodsWe conducted a retrospective chart review of patients with moderate-to-severe TBI evacuated out of combat theater to Landstuhl Regional Medical Center via Critical Care Air Transport Teams. We collected demographics, flight and injury information, procedures, oxygenation, and outcomes (discharge disposition and hospital/ICU/ventilator days). We categorized patients as having a CAR if they had a documented CAR or maximum cabin altitude of 5,000 feet or lower in their Critical Care Air Transport Teams record. We calculated descriptive statistics and constructed regression models to evaluate the association between CAR and clinical outcomes.ResultsWe reviewed the charts of 435 patients, 31% of which had a documented CAR. Nineteen percent of the sample had a PaO2 lower than 80 mm Hg, and 3% of patients experienced a SpO2 lower than 93% while in flight. When comparing preflight and in-flight events, we found that the percentage of patients who had a SpO2 of 93% or lower increased for the No CAR group, whereas the CAR group did not experience a significant change. However, flying without a CAR was not associated with discharge disposition, mortality, or hospital/ICU/ventilator days. Further, having a CAR was not associated with these outcomes after adjusting for additional flights, injury severity, injury type, or preflight head surgery.ConclusionsPatients with TBI who flew with a CAR did not differ in clinical outcomes from those without a CAR.
Teleophthalmology Prevents Unnecessary Evacuation from Warzone
Authors: Cocozza Victoria R , Santamaria Joseph A , Giles G Bryant , Keehn Marion C , Miller Kyle 
Published: June 2020

Abstract
The purpose of this case report is to highlight the benefits of using teleconsultation to diagnose ophthalmologic conditions within the restraints of a combat environment. A previously healthy 49-year-old service member deployed in the Middle East presented with diplopia over the course of 2 weeks. Initial diagnosis by his primary care physician upon partial ophthalmologic exam was a pupil-sparing CN III palsy without ptosis. Initial teleconsultation response from Naval Medical Center Portsmouth was within 6 hours. After an ophthalmologic sensorimotor examination was videotaped and sent to the referred ophthalmologist, teleconsultation was completed and discussed with the patient. The updated diagnosis was CN IV palsy with slight right hypertropia worse on left gaze—most likely congenital in origin. Upon further follow-up stateside, his final diagnosis was diplopia related to thyroid disease. Overall, his diagnosis remained a nonurgent condition that allowed the service member to remain at his duty station and prevent an unnecessary evacuation. Ultimately, teleconsultation provided many benefits for the service member and the U.S. military.
Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: A study by the fungal PCR Initiative and the Modimucor study group
Authors: Rocchi S , Scherer E , Mengoli C , Alanio A , Botterel F , Bougnoux M E , Bretagne S , Cogliati M , Cornu M , Dalle F , Damiani C , Denis J , Fuchs S , Gits-Muselli M , Hagen F , Halliday C , Hare R , Iriart X , Klaassen C , Lackner M , Lengerova M , Letscher-Bru V , Morio F , Nourrisson C , Posch W , Sendid B , Springer J , Willinger B , White P L , Barnes R A , Cruciani M , Donnelly J P , Loeffler J , Millon L 
Published: June 2020

Abstract
Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance of methods currently used. The participants comprised 12 laboratories from French university hospitals (nine of them participating in the Modimucor study) and 11 laboratories participating in the Fungal PCR Initiative. For panel 1, three sera were each spiked with DNA from three different species (Rhizomucor pusillus, Lichtheimia corymbifera, Rhizopus oryzae). For panel 2, six sera with three concentrations of R. pusillus and L. corymbifera (1, 10, and 100 genomes/ml) were prepared. Each panel included a blind negative-control serum. A form was distributed with each panel to collect results and required technical information, including DNA extraction method, sample volume used, DNA elution volume, qPCR method, qPCR template input volume, qPCR total reaction volume, qPCR platform, and qPCR reagents used. For panel 1, assessing 18 different protocols, qualitative results (positive or negative) were correct in 97% of cases (70/72). A very low interlaboratory variability in Cq values (SD = 1.89 cycles) were observed. For panel 2 assessing 26 different protocols, the detection rates were high (77–100%) for 5/6 of spiked serum. There was a significant association between the qPCR platform and performance. However, certain technical steps and optimal combinations of factors may also impact performance. The good reproducibility and performance demonstrated in this study support the use of Mucorales qPCR as part of the diagnostic strategy for mucormycosis.
In vivo mutagenicity evaluation of the soil fumigant 1,3-dichloropropene
Authors: Badding Melissa , Gollapudi B Bhaskar , Gehen Sean , Yan Zhongyu (June) 
Published: June 2020

Abstract
1,3-Dichloropropene (1,3-D; CAS No. 542-75-6) is a soil fumigant used for the control of nematodes in agriculture. There is an extensive database on the genotoxicity of 1,3-D and many of the published studies are confounded by the presence of mutagenic stabilisers in the test substance. Mixed results were obtained in the in vitro assays, often due to the purity of the 1,3-D sample tested. In order to get further clarity, the mutagenic potential of 1,3-D was investigated in vivo in the transgenic Big Blue rodent models. Inhalation exposure of 150 ppm 1,3-D (×2.5 tumourigenic dose) to transgenic male B6C3F1 mice did not induce lacI mutations in either the lung (tumour target tissue) or liver. Similarly, dietary administration of 1,3-D up to 50 mg/kg/day to transgenic male Fischer 344 rats did not increase the cII mutant frequency in either the liver (tumour target) or kidney. These results, along with other available in vivo data, including the absence of DNA adducts and clastogenic/aneugenic potential, support the conclusion that 1,3-D is efficiently detoxified in vivo and, as such, does not pose a mutagenic hazard or risk.
High-throughput methods for measuring DNA thermodynamics
Authors: Bae Jin H , Fang John Z , Zhang David Yu 
Published: June 2020

Abstract
Understanding the thermodynamics of DNA motifs is important for prediction and design of probes and primers, but melt curve analyses are low-throughput and produce inaccurate results for motifs such as bulges and mismatches. Here, we developed a new, accurate and high-throughput method for measuring DNA motif thermodynamics called TEEM (Toehold Exchange Energy Measurement). It is a refined framework of comparing two toehold exchange reactions, which are competitive strand displacement between oligonucleotides. In a single experiment, TEEM can measure over 1000 ΔG° values with standard error of roughly 0.05 kcal/mol.
Getting DNA and RNA out of the dark with 2CNqA: a bright adenine analogue and interbase FRET donor
Authors: Wypijewska del Nogal Anna , Füchtbauer Anders F , Bood Mattias , Nilsson Jesper R , Wranne Moa S , Sarangamath Sangamesh , Pfeiffer Pauline , Rajan Vinoth Sundar , El-Sagheer Afaf H , Dahlén Anders , Brown Tom , Grøtli Morten , Wilhelmsson L Marcus 
Published: June 2020

Abstract
With the central role of nucleic acids there is a need for development of fluorophores that facilitate the visualization of processes involving nucleic acids without perturbing their natural properties and behaviour. Here, we incorporate a new analogue of adenine, 2CNqA, into both DNA and RNA, and evaluate its nucleobase-mimicking and internal fluorophore capacities. We find that 2CNqA displays excellent photophysical properties in both nucleic acids, is highly specific for thymine/uracil, and maintains and slightly stabilises the canonical conformations of DNA and RNA duplexes. Moreover, the 2CNqA fluorophore has a quantum yield in single-stranded and duplex DNA ranging from 10% to 44% and 22% to 32%, respectively, and a slightly lower one (average 12%) inside duplex RNA. In combination with a comparatively strong molar absorptivity for this class of compounds, the resulting brightness of 2CNqA inside double-stranded DNA is the highest reported for a fluorescent base analogue. The high, relatively sequence-independent quantum yield in duplexes makes 2CNqA promising as a nucleic acid label and as an interbase Förster resonance energy transfer (FRET) donor. Finally, we report its excellent spectral overlap with the interbase FRET acceptors qAnitro and tCnitro, and demonstrate that these FRET pairs enable conformation studies of DNA and RNA.
Impact of the dialysate acid component on haemodialysis mortality rates
Authors: Couchoud Cécile , Hannedouche Thierry , Bauwens Marc , Ecochard René , Lassalle Mathilde , Frimat Luc , Choukroun Gabriel , Lobbedez Thierry 
Published: June 2020

AbstractBackgroundNo prospective study has evaluated the long-term effect on mortality of the new acid concentrates added to bicarbonate dialysate. The aim of this pharmacoepidemiological study was to evaluate the association between hydrochloric or citric acid–based dialysate and mortality on haemodialysis (HD).MethodsThis study included 117 796 patients with 3 723 887 months on HD recorded in the national French Renal Epidemiology and Information Network registry. Dialysate acid components were retrospectively reconstructed for each facility. All patients on HD were associated each month with an exposure based on that at their facility of treatment. We took each patient’s time-varying exposure into account to calculate the monthly mortality rates for each exposure. Incidence rate ratios (IRRs) for mortality were calculated with a Poisson regression, with acetic acid as the reference. Regressions were adjusted for initial clinical characteristics (age, gender, previous cardiovascular events, active malignancy, diabetes, pulmonary disease, mobility), dialysis technique and location (in-centre, outpatient centre, self-care unit) and ESRD vintage, updated monthly.ResultsThe crude mortality rate per 1000 patient-months with citric acid {11.5 [95% confidence interval (CI) 11.1–12.0]} was lower than with either acetic acid [12.9 (95% CI 12.8–13.1)] or hydrochloric acid [12.8 (95% CI 12.2–13.5)]. For the 2014–17 period, the IRR for mortality with citric acid [adjusted IRR 0.94 (95% CI 0.90–0.99)] and with hydrochloric acid [adjusted IRR 0.86 (95% CI 0.79–0.94)] were significantly lower than with acetic acid.ConclusionThis post-marketing study of long-term exposure to dialysate acidifiers at the patient level found the use of citric and hydrochloric acid–based dialysates, compared with acetic acid, was associated with lower mortality.
Urine interleukin-9 and tumor necrosis factor-α for prognosis of human acute interstitial nephritis
Authors: Moledina Dennis G , Wilson F Perry , Kukova Lidiya , Obeid Wassim , Luciano Randy , Kuperman Michael , Moeckel Gilbert W , Kashgarian Michael , Perazella Mark A , Cantley Lloyd G , Parikh Chirag R 
Published: June 2020

Abstract

Background
We previously demonstrated that urine interleukin (IL)-9 and tumor necrosis factor (TNF)-α can distinguish acute interstitial nephritis (AIN) from other causes of acute kidney injury. Here we evaluated the role of these biomarkers to prognosticate kidney function in patients with AIN.

Methods
In a cohort of participants with biopsy-proven, adjudicated AIN, we tested the association of histological features and urine biomarkers (IL-9 and TNF-α) with estimated glomerular filtration rate measured 6 months after diagnosis (6 m-eGFR) controlling for eGFR before AIN and albuminuria. We also evaluated subgroups in whom corticosteroid use was associated with 6 m-eGFR.

Results
In the 51 (93%) of the 55 participants with complete data, median (interquartile range) eGFR before and 6 m after AIN were 41 (27–69) and 28 (13–47) mL/min/1.73 m2, respectively. Patients with higher severity of interstitial fibrosis had lower 6 m-eGFR, whereas those with higher tubulointerstitial infiltrate had higher 6 m-eGFR. IL-9 levels were associated with lower 6 m-eGFR only in the subset of patients who did not receive corticosteroids [6m-eGFR per doubling of IL-9, −6.0 (−9.4 to −2.6) mL/min/1.73 m2]. Corticosteroid use was associated with higher 6 m-eGFR [20.9 (0.2, 41.6) mL/min/1.73 m2] only in those with urine IL-9 above the median (&gt;0.66 ng/g) but not in others.

Conclusions
Urine IL-9 was associated with lower 6 m-eGFR only in participants not treated with corticosteroids. Corticosteroid use was associated with higher 6 m-eGFR in those with high urine IL-9. These findings provide a framework for IL-9-guided clinical trials to test efficacy of immunosuppressive therapy in patients with AIN.
Marriage and housework
Authors: Borra Cristina , Browning Martin , Sevilla Almudena 
Published: June 2020

AbstractThis article provides insights into the gains of forming a couple by estimating how much of the difference in housework between single and married individuals is causal and how much is due to selection. Time-varying observed variables and time-invariant heterogeneity explains about half of the observed differences in housework documented in the cross-sectional data. There remains a genuine one-and-a-half-hour increase per week in housework time for each partner, with women specializing in routine and men in non-routine housework tasks.
The impact of the COVID-19 pandemic on suicide rates
Authors: Sher Leo 
Published: June 2020

SummaryMultiple lines of evidence indicate that the coronavirus disease 2019 (COVID-19) pandemic has profound psychological and social effects. The psychological sequelae of the pandemic will probably persist for months and years to come. Studies indicate that the COVID-19 pandemic is associated with distress, anxiety, fear of contagion, depression and insomnia in the general population and among healthcare professionals. Social isolation, anxiety, fear of contagion, uncertainty, chronic stress and economic difficulties may lead to the development or exacerbation of depressive, anxiety, substance use and other psychiatric disorders in vulnerable populations including individuals with pre-existing psychiatric disorders and people who reside in high COVID-19 prevalence areas. Stress-related psychiatric conditions including mood and substance use disorders are associated with suicidal behavior. COVID-19 survivors may also be at elevated suicide risk. The COVID-19 crisis may increase suicide rates during and after the pandemic. Mental health consequences of the COVID-19 crisis including suicidal behavior are likely to be present for a long time and peak later than the actual pandemic. To reduce suicides during the COVID-19 crisis, it is imperative to decrease stress, anxiety, fears and loneliness in the general population. There should be traditional and social media campaigns to promote mental health and reduce distress. Active outreach is necessary, especially for people with a history of psychiatric disorders, COVID-19 survivors and older adults. Research studies are needed of how mental health consequences can be mitigated during and after the COVID-19 pandemic.
Identity Politics and Trade Policy
Authors: Grossman Gene M , Helpman Elhanan 
Published: June 2020

AbstractWe characterize trade policies that result from political competition when assessments of well-being include both material and psychosocial components. The material component reflects, as usual, satisfaction from consumption. Borrowing from social identity theory, we take the psychosocial component as combining the pride and self-esteem an individual draws from the status of groups with which she identifies and a dissonance cost she bears from identifying with those that are different from herself. In this framework, changes in social identification patterns that may result, for example, from increased income inequality or heightened class or ethnic tensions, lead to pronounced changes in trade policy. We analyse the nature of these policy changes.
Biomarker profiles of endothelial activation and dysfunction in rare systemic autoimmune diseases: implications for cardiovascular risk
Authors: Wienke Judith , Mertens Jorre S , Garcia Samuel , Lim Johan , Wijngaarde Camiel A , Yeo Joo Guan , Meyer Alain , van den Hoogen Lucas L , Tekstra Janneke , Hoogendijk Jessica E , Otten Henny G , Fritsch-Stork Ruth D E , de Jager Wilco , Seyger Marieke M B , Thurlings Rogier M , de Jong Elke M G J , van der Kooi Anneke J , van der Pol W Ludo ,   Dutch Juvenile Myositis Consortium, Arkachaisri Thaschawee , Radstake Timothy R D J , van Royen-Kerkhof Annet , van Wijk Femke 
Published: June 2020

Abstract

Objectives
Vasculopathy is an important hallmark of systemic chronic inflammatory connective tissue diseases (CICTD) and is associated with increased cardiovascular risk. We investigated disease-specific biomarker profiles associated with endothelial dysfunction, angiogenic homeostasis and (tissue) inflammation, and their relation to disease activity in rare CICTD.

Methods
A total of 38 serum proteins associated with endothelial (dys)function and inflammation were measured by multiplex-immunoassay in treatment-naive patients with localized scleroderma (LoS, 30), eosinophilic fasciitis (EF, 8) or (juvenile) dermatomyositis (34), 119 (follow-up) samples during treatment, and 65 controls. Data were analysed by unsupervised clustering, Spearman correlations, non-parametric t test and ANOVA.

Results
The systemic CICTD, EF and dermatomyositis, had distinct biomarker profiles, with ‘signature’ markers galectin-9 (dermatomyositis) and CCL4, CCL18, CXCL9, fetuin, fibronectin, galectin-1 and TSP-1 (EF). In LoS, CCL18, CXCL9 and CXCL10 were subtly increased. Furthermore, dermatomyositis and EF shared upregulation of markers related to interferon (CCL2, CXCL10), endothelial activation (VCAM-1), inhibition of angiogenesis (angiopoietin-2, sVEGFR-1) and inflammation/leucocyte chemo-attraction (CCL19, CXCL13, IL-18, YKL-40), as well as disturbance of the Angiopoietin-Tie receptor system and VEGF-VEGFR system. These profiles were related to disease activity, and largely normalized during treatment. However, a subgroup of CICTD patients showed continued elevation of CXCL10, CXCL13, galectin-9, IL-18, TNFR2, VCAM-1, and/or YKL-40 during clinically inactive disease, possibly indicating subclinical interferon-driven inflammation and/or endothelial dysfunction.

Conclusion
CICTD-specific biomarker profiles revealed an anti-angiogenic, interferon-driven environment during active disease, with incomplete normalization under treatment. This warrants further investigation into monitoring of vascular biomarkers during clinical follow-up, or targeted interventions to minimize cardiovascular risk in the long term.
‘She Used to Doctor Us up Herself’: Patent Medicines, Mothers, and Expertise in Early Twentieth-Century Britain
Authors: Bramwell Erin Elizabeth 
Published: June 2020

Abstract
This article provides much-needed new insights into how patent medicines could enhance maternal power outside of clinical environments in the early twentieth century. While historians have considered the maternal management of domestic health, the role patent medicine products played in this complex equation of authority and expertise has yet to be the subject of sustained historical analysis. Using oral history archives, memoirs, social commentary, and advertisements, this article demonstrates how the expertise that mothers possessed was multifaceted, with powerful spatial, temporal, and even commercial connotations. This influence was especially significant at a time when clinical environments were still largely dominated by men, when the home was increasingly invaded by experts and expertise, and when dependants of wage earners were not covered by welfare provisions such as the 1911 National Insurance Act.
Effects of Arsenic (+3 Oxidation State) Methyltransferase Gene Polymorphisms and Expression on Bladder Cancer: Evidence from a Systematic Review, Meta-analysis and TCGA Dataset
Authors: Song Yuxuan , Jin Donghui , Chen Jingyi , Liang Wanfeng , Liu Xiaoqiang 
Published: June 2020

AbstractInorganic arsenic (iAs) is a recognized environment-related factor for bladder cancer (BCa). Arsenic (+3 oxidation state) methyltransferase (AS3MT) gene might influence BCa by regulating iAs metabolism. The aim of the present study was to explore whether AS3MT polymorphisms could affect BCa susceptibility. We systematically reviewed eligible case-control studies about AS3MT polymorphisms and BCa and to further compare the genotype distribution and allele distribution between BCa patients and controls by meta-analysis for humans. Besides, to clarify the effects of AS3MT expression on BCa clinical outcomes and survival time, we also conducted a series of analyses based on The Cancer Genome Atlas dataset. Databases were systematically retrieved and we applied Stata software to perform meta-analysis. The registration of this study protocol is at PROSPERO and ID is CRD42019133947. Five articles were recruited and pooled results demonstrated that rs3740393 and rs11191438 polymorphisms were related to BCa risk in overall population (p &lt; .05) in the overall population. In addition, GG and GC genotypes in rs3740393 and GG genotype in rs11191438 might be the susceptibility genotypes for BCa. Results based on 168 BCa samples from TGCA indicated that patients with higher expression of AS3MT had poor overall survival time and AS3MT expression is an independent indicator for BCa survival. This study identified that AS3MT polymorphisms could affect BCa risk and AS3MT expression was pivotal in prognosis of BCa.