bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide Identification of Powdery Mildew Resistance in Common Bean
Authors: Binagwa Papias Hongera , Traore Sy M. , Egnin Marceline , Bernard Gregory C. , Ritte Inocent , Mortley Desmond , Kamfwa Kelvin , He Guohao , Bonsi Conrad C. 
Published: June 2020

Abstract
Background

Genome-wide association studies (GWAS) was utilized to detect genetic variations related to the powdery mildew (PM) resistance and several agronomic traits in common bean. However, its application in common bean and the PM interactions to identify genes and their location in the common bean genome has not been fully addressed.
Results

Genome-wide association studies (GWAS) through marker-trait association are useful molecular tools for identification of disease resistance and other agronomic traits. SNP genotyping with a BeadChip containing 5398 SNPs was used to detect genetic variations related to resistance to PM disease in a panel of 206 genotypes grown under field conditions for two consecutive years. Significant SNPs identified on chromosome 4 and 10 were repeatable, confirming the reliability of the phenotypic data scored from the genotypes grown in two locations within two years. A cluster of resistance genes was revealed on the chromosome 4 of common bean genome among which CNL and TNL like resistance genes were identified. Furthermore, two resistance genes Phavu_010G1320001g and Phavu_010G136800g were also identified on pv10; further sequence analysis showed that these genes were homologs to the Arabidopsis disease resistance protein (RLM1A-like) and the putative disease resistance protein (At4g11170.1), respectively. Two LRR receptor-like kinases (RLK) were also identified on pv11 in samples collected in 2018 only. Many genes encoding auxin-responsive protein, TIFY10A protein, growth-regulating factor 5-like, ubiquitin-like protein, cell wall protein RBR3-like protein related to PM resistance were identified nearby significant SNPs. These results suggested that the resistance to PM pathogen involves a network of many genes constitutively co-expressed and may generate several layers of defense barriers or inducible reactions.
Conclusion

Our results provide new insights into common bean and PM interactions, and revealed putative resistance genes as well as their location on common bean genome that could be used for marker-assisted selection, functional genomic study approaches to confirm the role of these putative genes; hence, developing common bean resistance lines to the PM disease.
Prevalence, associated factors, and outcomes for labor induction at a tertiary hospital in Northern Tanzania: A retrospective cohort study 2000 - 2015
Authors: Tarimo Clifford Silver , Wu Jian , Obure Joseph , Mahande Michael Johnson 
Published: June 2020

Abstract
Background

Labor induction (IOL) refers to an obstetric intervention which include artificial stimulation of uterine contraction aiming at vaginal delivery of a fetus before the onset of spontaneous labor. Despite undisputed importance of this intervention in improving pregnancy outcomes, data on its utilization and outcomes in Tanzania is limited. The aim of this study was to determine the prevalence, associated factors and outcomes for labor induction among women who were attended at a tertiary hospital in north-Tanzania.
Methods

We designed a retrospective cohort study and analyzed 53338 deliveries at the Kilimanjaro Christian Medical Centre (KCMC) between the year 2000 to 2015. We enrolled singleton deliveries with vertex presentation and excluded observations with missing information on induction status. Relative risk and 95% Confidence Interval for risk factors and outcomes of labor induction were estimated using log-binomial regression models. Robust variance estimation was used to account for repeated deliveries from the same subject.
Results

53,338 deliveries were analyzed. Prevalence of labor induction was 21.63%. Independent risk factors for labor induction were; postdates (RR = 1.21; 95% CI: 1.15–1.28), fetal macrosomia (RR = 1.27; 95% CI: 1.18–1.36) and obesity (RR = 1.12; 95% CI: 1.06–1.18). Labor induction was associated with an increased risk of uterine rapture (RR = 1.84; 95% CI: 1.62–2.09) and low (< 7) Apgar score (RR = 1.27; 95% CI: 1.17–1.37). Labor induction offered protective effect against cesarean delivery (RR = 0.56; 95% CI: 0.53–0.58) and admission to neonatal intensive care unit (RR = 0.94; 95% CI: 0.89–0.99).
Conclusion

Efforts aimed at achieving the health-related sustainable development goals should focus on increasing access to effective interventions as well as improving quality of health care while being cautioned on likelihood of adverse maternal and fetal outcomes.
A comparison of two-dimensional and three-dimensional techniques for determining the kinematic patterns for hindlimb obstacle clearance during sheep locomotion.
Authors: Diogo Camila Cardoso , Fonseca Bárbara , Almeida Francisca S.M. , Costa Luís Maltez da , Pereira José Eduardo , Filipe Vitor , Couto Pedro Alexandre , Geuna Stefano , Armada-da-Silva Paulo A. , Maurício Ana Colette , Varejão Artur S.P. 
Published: June 2020

Abstract
Background: Analysis of locomotion is often used as a measure for impairment and recovery following experimental peripheral nerve injury. Compared to rodents, sheep offer several attractive features as an experimental model for studying peripheral nerve regeneration. There are no studies on locomotion outcomes after peripheral nerve injury and repair in the sheep model. In the present study, we performed and compared two-dimensional (2D) and, for the first time, three-dimensional (3D) hindlimb kinematics during obstacle avoidance in the ovine model. This study aimed to obtain kinematic data to serve as a template for an objective assessment of the ankle joint motion in future studies of common peroneal nerve (CP) injury and repair in the ovine model. Results: The strategy used by the sheep to bring the hindlimb over a moderately high obstacle, set to 10% of its hindlimb length, was the pronounced knee, ankle and metatarsophalangeal flexion when approaching and clearing the obstacle. Despite the overall time course kinematic patterns about the hip, knee, ankle, and metatarsophalangeal were identical, we found significant differences between values of the 2D and 3D joint angular motion. Conclusions: Our results show that the most apparent changes that occurred during the gait cycle were for the ankle and metatarsophalangeal joints, whereas the hip and knee joints were much less affected. Data and techniques described here are likely to be useful for an objective assessment of altered gait after CP injury and repair in an ovine model.
Chromosomal Abnormality in Fetuses with Isolated Antenatal Hydronephrosis: Update for Prenatal Diagnosis and Genetic Counseling
Authors: Zhou Xiaoyan , Wang Yan , Meng Lulu , Tan Jianxin , Qiao Fengchang , Hu Ping , Xu Zhengfeng 
Published: June 2020

Abstract
Background: The prenatal finding of fetuses with antenatal hydronephrosis (ANH) gives a significant dilemma for the clinicians. Which patients require invasive prenatal diagnosis? Though previous literatures have recommended the use of chromosomal microarray analysis (CMA)for fetuses with CAKUT, the cutoff value for CMA have no current consensus on fetuses with ANH. In this article, we aimed to detect chromosomal abnormalities in fetuses with isolated severe ANH (anterior-posterior renal pelvic diameter (APRPD) ≥ 10mm) by CMA, summarized the literatures and proposed recommendations for the prenatal genetic diagnosis according to APRPD.Methods: Fetuses (n=84) with isolated severe ANH (APRPD ≥ 10mm) were evaluated by CMA. According to APRPD measurements at second trimester, we classified the cases into two groups: (1) Group A: cases with APRPD of 10–15 mm(N=57); (2) Group B: cases with APRPD ≥ 15 mm(N=27).The prenatal and postnatal outcomes were assessed by ultrasonic examination and telephone follow-up.Results: Overall, one case with 18 trisomy was identified. Clinically significant copy number variants (pathogenic or likely pathogenic CNVs) were identified in 11.9% (10/84) cases, including 3.5% (3/84) of pathogenic CNVs. The detection rates were 5.2% (3/57), 25.9% (7/27) for group A and group B, respectively. There was statistically significant differences in the frequency of clinic significant CNVs in the two groups (p＜0.05). Conclusion: CMA is valuable in prenatal genetic diagnosis of fetuses with severe ANH（APRPD ≥ 10mm）, regardless of whether other ultrasonic abnormalities were observed. This cohort should be followed up during the pregnancy.
Genome-wide analysis and expression characterization of zinc finger homeodomain (ZHD) family genes responsed to different abiotic stresses and hormonal treatments in grape (Vitis vinifera L.)
Authors: Nai Guojie , Mao Juan , Lu Shixiong , Li Yanmei , Ma Zonghuan , Abdul khaliq , Chen Baihong 
Published: June 2020

Abstract
Background

Zinc finger homeodomain (ZHD) proteins transcription factors (TFs) family occupy a crucial role in regulating plant growth and development. The ZHD TFs family had been studied in diverse organisms such as humans, Drosophila, nematode worms and plants. Especially in plants there is an extensive exploration involved in photosynthesis, defense mechanisms and responses to stress. In this further research, we will provide a better understanding of the ZHD gene family in grape.
Results

In this study, a total of 12 VvZHD genes were identified from the Grape Genome. The exploration of detailed bioinformatics found that the full length of VvZHD genes ranges from 150 bp (base pair) to 26706 bp, isoelectric points (pIs) were 6.25–9.68, molecular weights (MWs) ranged from 10.28 kilodaltons (KDa) to 97.93 kDa. Also, analysis of phylogenetic evolution showed there existed a common ancestor between Arabidopsis and grape. Ka (Nonsynonymous) / Ks (Synonymous) was lower than 1 and strong collinearity showed there were tandem duplications of genes between Arabidopsis and grape. Codon bias levels of ZHD gene family were relatively higher overall. Gene chip data analysis of cabernet sauvignon seedlings showed VvZHD genes had different expression under different time of different treatments, and with the prolongation of treatment time, most of VvZHD genes were slightly downregulated. The qRT-PCR was carried out under different hormones and stresses to verify expression profiles in grape roots and leaves. Results revealed that most VvZHD genes had higher expressions in roots but lower expressions in leaves. Also, most of the VvZHD gene members were responsed with high expressions after the treatment of ABA, IAA and MeJA in roots. However, VvZHD9, VvZHD11 and VvZHD12 were highly expressed by inducing of PEG in leaves.
Conclusions

Through genome-wide analysis and expression characterization of ZHD family genes, results showed 12 members had the diversity of function, structure and unique expression characterization under various abiotic stress and hormone treatments. This study will provide us with a newer insight on ZHD gene family of grape and promote to explore the biological functions of ZHD genes in other plants.
Identification of a novel circRNA, hsa_circ_0065898, that regulates tumor growth in cervical squamous cell carcinoma
Authors: Li Ni , Liu Jie , Deng Xiaohui 
Published: June 2020

Abstract
Background: Circular RNAs (circRNAs) have been reported to play an important role in regulating tumor pathogenesis and progression. The molecular mechanism of circRNAs in cervical squamous cell carcinoma (CSCC) remains poorly understood. We aimed to identify the circRNAs differentially expressed, and to investigate the role of a novel circRNA, hsa_circ_0065898, in regulating proliferation, migration, and invasion in CSCC.Methods: The online Kaplan-Meier Plotter was used to analyze the relationship between miRNA expression and overall survival. Bioinformatics tools, such as R, Cytoscape, and Perl, were used to analyze the Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, protein-protein interaction (PPI) network, and regulatory network. The expression level of hsa_circ_0065898 in CSCC cell lines was evaluated using quantitative polymerase chain reaction in vitro. The cell counting kit-8 (CCK-8) and transwell assays were used to assess cell proliferation, migration, and invasion. Results: circRNA expression data (GSE102686) was downloaded from the Gene Expression Omnibus database, and this included data from 5 CSCC patients and 5 normal tissues. Thirteen differentially expressed circRNAs were identified, which included 9 upregulated circRNAs and 4 downregulated circRNAs. GO enrichment analysis showed that the target genes of miRNAs associated with hsa_circ_0065898 were enriched in ubiquitin-protein transferase activity, ubiquitin-like protein transferase activity, core promoter sequence-specific DNA binding, mRNA 3′-UTR AU-rich region binding, core promoter binding, AU-rich element binding, ubiquitin-like protein ligase activity, and transcription corepressor activity. KEGG results showed that the Hippo and p53 signaling pathways played significant role in the pathway network. Hsa_circ_0065898 was significantly overexpressed in the CSCC cell lines. Hsa_circ_0065898 facilitated cell proliferation, migration, and invasion in CSCC.Conclusions: This study identified differentially expressed circRNAs and constructed the regulatory network of hsa_circ_0065898 targeting microRNAs and mRNAs. We demonstrated that hsa_circ_0065898 promoted CSCC cell proliferation, migration, and invasion. Hence, hsa_circ_0065898 might be useful as a biomarker for CSCC diagnosis and targeted therapy.
Molecular Epidemiology of SARS-CoV-2 Clusters Caused by Asymptomatic Cases in Anhui Province, China
Authors: yuan yuan , He Jun , Gong Lei , Li Weiwei , Jiang Liangliang , Liu Jiang , Chen Qingqing , Yu Junling , Hou Sai , Shi Yonglin , Lu Siqi , Zhang Zhuhui , Ge Yinglu , Sa Nan , He Lan , Wu Jiabing , Sun Yong , Liu Zhirong 
Published: June 2020

Abstract
Background: COVID-19 is a newly emerging disease caused by a novel coronavirus (SARS-CoV-2), which spread globally in early 2020. Asymptomatic carriers of the virus contribute to the propagation of this disease, and the existence of asymptomatic infection has caused widespread fear and concern in the control of this pandemic. Methods: In this study, we investigated the origin and transmission route of SARS-CoV-2 in Anhui’s two clusters, analyzed the role and infectiousness of asymptomatic patients in disease transmission, and characterized the complete spike gene sequences in the Anhui strains. Results: We conducted an epidemiological investigation of two clusters caused by asymptomatic infections sequenced the spike gene of viruses isolated from 12 patients. All cases of the two clusters we investigated had clear contact histories, both from Wuhan, Hubei province. The viruses isolated from two outbreaks in Anhui were found to show a genetically close link to the virus from Wuhan. In addition, new single nucleotide variations were discovered in the spike gene. Conclusions: Both clusters may have resulted from close contact and droplet-spreading and asymptomatic infections were identified as the initial cause. We also analyzed the infectiousness of asymptomatic cases and the challenges to the current epidemic to provided information for the development of control strategies.
Lumbosacral fusion terminated to S1 for adult degenerative scoliosis: Technical innovation and finite element analysis
Authors: Zhao Zheng , Hu Huiqiang , Guo Jianwei , Dai Kun , Wang Chaoying , Du Yukun , Li Jianyi , Xi Yongming 
Published: June 2020

Abstract
Background: To evaluated the practicability and reliability of CBT+TT in the treatment of ADS by finite element analysis and the comparison of clinical effects.Methods: 18 cases were enrolled from patients who were admitted to the department of Spine Surgery in Affiliated Hospital of Qingdao University with a strict standard which qualify for the surgical indications of L5-S1 long segment fusion. Based on different spinal-pelvic fusion techniques, those cases were divided into three groups including the groups of IS , S2AI and CBT+TT, 3, 4 and 11 cases recpectively. Clinical data of CBT+TT group such as VAS, ODI, coronal Codd angle, C7PL-CSVL and SVA were acquired preoperatively and compared with those measured at the end of follow-up. DICOM data of CT scan (1mm) of all postoperative patients in those 3 groups were used to created 3D models. The models were imported into software for the finite element analysis under different conditions such as flexion, extension, lateral flexion and rotation, to obtain the stress distribution properties of three fusion techniques.Results: In CBT+TT group, the VAS score was 7.00±0.89 preoperatively and 0.91±0.70 at the end of follow-up. The ODI was 51.09±7.83% and 5.45±1.13%. There was a significant difference between the preoperative data and that at the end of follow-up (P<0.001). The Cobb angle was 49.10±11.51° and 12.33±3.96°. C7PL-CSVL was 27.27±17.61 mm and 12.20±8.04 mm. SVA was 25.33±18.21 mm and 8.60±5.31 mm. The results of the finite element analysis of simulated conditions, such as flexion, extension, lateral flexion and rotation, showed that in the IS group, the average extreme pressure value was 366.1 MPa, 183.1 MPa, 398.4 MPa, and 573.6 MPa; in the S2AI group, the values were 518.4 MPa, 259.2 MPa, 608.2 MPa, and 545.7 MPa; and in the CBT+TT group, the values were 390.0 MPa, 195.0 MPa, 353.7 MPa, and 476.8 MPa. Conclusion: This innovative technique (CBT+TT, terminating at S1) has been proven to be reliable and practical in the treatment of degenerative scoliosis.
Similar outcomes with first-line observation versus metastasectomy for bone giant cell tumor lung metastases
Authors: Tsukamoto Shinji , Ciani Giovanni , Mavrogenis Andreas F. , Ferrari Cristina , Akahane Manabu , Tanaka Yasuhito , Rocca Michele , Longhi Alessandra , Donati Davide Maria , Errani Costantino 
Published: June 2020

Abstract
Background: The outcome of lung metastases in patients with giant cell tumor of bone (GCTB) varies from spontaneous regression to uncontrolled growth. To investigate whether observation is an appropriate first-line management for patients with lung metastases from GCTB, we compared the outcomes of patients initially treated with observation with those treated with metastasectomy.Methods: We retrospectively reviewed the data of 29 patients with lung metastases from histologically confirmed GCTB. The median follow-up period was 114 months. We evaluated progression-free survival, which was defined as the time from the date of occurrence of lung metastases to the date of disease progression in the observation or incomplete metastasectomy group, disease recurrence in the complete metastasectomy group, or the last follow-up.Results: Disease progression or recurrence occurred in 14 patients (48.3%). Progression-free survival did not vary significantly between the observation and metastasectomy groups (p=0.373). The number of metastasectomies was significantly higher in the initial metastasectomy group than in the observation group (p=0.017).Conclusions: Observation can be used safely as first-line management for patients with lung metastases from GCTB with an outcome similar to that of metastasectomy.
Patients and relatives as auditors of safe practices
Authors: Rodrigo-Rincon Maria Isabel , Aristorena Isabel Irigoyen , Tirapu-Leon Belén , Barcala Nicolas Zaballos , Carricas Maite Sarobe , Lobo-Palanco Joaquin , Caamaño Maria Luisa Antelo , Martin-Vizcaino Marta , Burnett Susan 
Published: June 2020

Abstract
Background When there is a gap in professionals’ adherence to safe practices during cancer treatment, the consequences can be serious. Identifying these gaps in order to enable improvements in patient safety can be a challenge. This study aimed to assess if cancer patients and their relatives can be given the skills to audit four safe practices reliably, and to explore whether they are willing to play this new role.Methods We recruited 136 participants in 2018, from the oncology and haematology day hospital of a tertiary hospital in Spain.The study comprised two parts: an interventional educational program and a cross-sectional design to collect data on the participants’ skills and perceptions about their willingness to be auditors.Results 63 participants (46.3%) were classified as good auditors after the training. A logistic regression model identified age, educational level, previous experience of an adverse event and perception of hospital safety as predictor variables. Additionally, 106 (77.9%) participants said that they would like to anonymously audit the professionals’ compliance of at least three of four safe practices. The willingness to audit safe practices was differed depending on the safe practice but these differences did not reach statistical significance.Conclusion The data gathered by patients and relatives acting as auditors can provide healthcare organizations with valuable information about safety and quality of care that is not accessible otherwise. This new role provides an innovative way to engage patients and their families (P&Fs) in healthcare safety where other methods have not had success. The paper sets out the methods that healthcare organizations need to undertake to enrol and train patients and relatives in an auditor role.
Prevalence of khat chewing during pregnancy in Ethiopia: a systematic review and meta-analysis
Authors: Bifftu Berhanu Boru 
Published: June 2020

Abstract
Background

Khat (Catha edulis) is a flowering green plant mainly chewed for recreational. Khat chewing is becoming on rising at alarming rate and adversely affects the socio-economic, psycho-social, and physical health including the health of the fetus. Thus, this study aimed to determine the pooled prevalence of khat chewing during pregnancy and its associated factors.
Methods

Databases: PubMed/Medline, CINAHL, SCOPUS and AJOL were searched. Weighted inverse variance random-effects model was used to calculate the pooled prevalence of depression. Heterogeneity across the studies was assessed by Cochrane chi-square and quantified by I2 statistics test. Funnel plots and Egger’s test were used to determine publication bias. Sensitivity test and subgroup analysis were also performed.
Results

In this study, a total of 9,915 study participants were included from 22 studies. The pooled prevalence of khat chewing was found to be 20% (95% CI; 14%-27%). Tests of heterogeneity demonstrated evidence of heterogeneity (I2 = 98.46%, p < 0.001). However, the analysis of sensitivity tests and publication bias showed absence of influential study and publication bias [Egger’s test (P = 0.02)]. Factors such as: being Muslim, lower educational status, family history of mental illness, and partner previous history of khat chewing were associated with khat chewing.
Conclusion

One in five pregnant women has history of khat chewing. Thus, authors’ suggest the need of screening for khat chewing as an integrated part of Antenatal care.