bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reproductive performance of gilthead seabream (Sparus aurata) broodstock showing different expression of fatty acyl desaturase 2 and fed two dietary fatty acid profiles
Authors: Ferosekhan Shajahan , Xu Hanlin , Turkmen Serhat , Gómez Ana , Afonso Juan Manuel , Fontanillas Ramon , Rosenlund Grethe , Kaushik Sadasivam , Izquierdo Marisol 
Published: September 2020

AbstractPrevious studies have shown that it is possible to nutritionally program gilthead seabream offspring through fish oil (FO) replacement by vegetable oils (VO) in the broodstock diet, to improve their ability to grow fast when fed low fish meal (FM) and FO diets during grow-out phase. However, in those studies broodstock performance was reduced by the VO contained diet. Therefore, the present study aimed to determine if it is possible to replace FO by a mixture of FO and rapeseed oil (RO) with a specific fatty acid profile in broodstock diets, without altering gilthead seabream broodstock reproductive performance. Besides, the study also aimed to evaluate the reproductive performance of broodstock with different expression of fatty acid desaturase 2 gene (fads2) a key enzyme in synthesis of long chain polyunsaturated fatty acids. For that purpose, broodfish having either a high (HD) or low (LD) expression of fads2 were fed for three months during the spawning season with two diets containing different fatty acid profiles and their effects on reproductive hormones, fecundity, sperm and egg quality, egg biochemical composition and fads2 expression were studied. The results showed that blood fads2 expression in females, which tended to be higher than in males, was positively related to plasma 17β-estradiol levels. Moreover, broodstock with high blood fads2 expression showed a better reproductive performance, in terms of fecundity and sperm and egg quality, which was correlated with female fads2 expression. Our data also showed that it is feasible to reduce ARA, EPA and DHA down to 0.43, 6.6 and 8.4% total fatty acids, respectively, in broodstock diets designed to induce nutritional programming effects in the offspring without adverse effects on spawning quality. Further studies are being conducted to test the offspring with low FM and FO diets along life span.
Oxidative switch drives mitophagy defects in dopaminergic parkin mutant patient neurons
Authors: Schwartzentruber Aurelie , Boschian Camilla , Lopes Fernanda Martins , Myszczynska Monika A. , New Elizabeth J. , Beyrath Julien , Smeitink Jan , Ferraiuolo Laura , Mortiboys Heather 
Published: September 2020

AbstractMutations in PRKN are the most common cause of early onset Parkinson’s disease. Parkin is an E3 ubiquitin ligase, functioning in mitophagy. Mitochondrial abnormalities are present in PRKN mutant models. Patient derived neurons are a promising model in which to study pathogenic mechanisms and therapeutic targets. Here we generate induced neuronal progenitor cells from PRKN mutant patient fibroblasts with a high dopaminergic neuron yield. We reveal changing mitochondrial phenotypes as neurons undergo a metabolic switch during differentiation. Fibroblasts from 4 controls and 4 PRKN mutant patients were transformed into induced neuronal progenitor cells and subsequently differentiated into dopaminergic neurons. Mitochondrial morphology, function and mitophagy were evaluated using live cell fluorescent imaging, cellular ATP and reactive oxygen species production quantification. Direct conversion of control and PRKN mutant patient fibroblasts results in induced neuronal progenitor and their differentiation yields high percentage of dopaminergic neurons. We were able to observe changing mitochondrial phenotypes as neurons undergo a metabolic switch during differentiation. Our results show that when pre-neurons are glycolytic early in differentiation mitophagy is unimpaired by PRKN deficiency. However as neurons become oxidative phosphorylation dependent, mitophagy is severely impaired in the PRKN mutant patient neurons. These changes correlate with changes in mitochondrial function and morphology; resulting in lower neuron yield and altered neuronal morphology. Induced neuronal progenitor cell conversion can produce a high yield of dopaminergic neurons. The mitochondrial phenotype, including mitophagy status, is highly dependent on the metabolic status of the cell. Only when neurons are oxidative phosphorylation reliant the extent of mitochondrial abnormalities are identified. These data provide insight into cell specific effects of PRKN mutations, in particular in relation to mitophagy dependent disease phenotypes and provide avenues for alternative therapeutic approaches.
Genome-wide identification and expression analysis of bZIP gene family in Carthamus tinctorius L.
Authors: Li Haoyang , Li Lixia , ShangGuan Guodong , Jia Chang , Deng Sinan , Noman Muhammad , Liu Yilin , Guo Yongxin , Han Long , Zhang Xiaomei , Dong Yuanyuan , Ahmad Naveed , Du Linna , Li Haiyan , Yang Jing 
Published: September 2020

AbstractThe basic leucine zipper (bZIP) is a widely known transcription factors family in eukaryotes. In plants, the role of bZIP proteins are crucial in various biological functions such as plant growth and development, seed maturation, response to light signal and environmental stress. To date, bZIP protein family has been comprehensively identified in Arabidopsis, castor, rice, ramie, soybean and other plant species, however, the complete genome-wide investigation of Carthamus tinctorius-bZIP family still remains unexplained. Here, we identified 52 putative bZIP genes from Carthamus tinctorius using a draft genome assembly and further analyzed their evolutionary classification, physicochemical properties, Conserved domain analysis, functional differentiation and the investigation of expression level in different tissues. Based on the common bZIP domain, CtbZIP family were clustered into 12 subfamilies renamed as (A–J, S, X), of which the X is a unique subfamily to Carthamus tinctorius. A total of 20 conserved protein motifs were found in CtbZIP proteins. The expression profiling of CtbZIP genes deciphered their tissue-specific pattern. Furthermore, the changes in CtbZIP transcript abundance suggested that their transcription regulation could be highly influenced by light intensity and hormones. Collectively, this study highlights all functional and regulatory elements of bZIP transcription factors family in Carthamus tinctorius which may serve as potential candidates for functional characterization in future.
The oil removal and the characteristics of changes in the composition of bacteria based on the oily sludge bioelectrochemical system
Authors: Guo Haiying , Tang Shanfa , Xie Shuixiang , Wang Penghua , Huang Chunfeng , Geng Xiaoheng , Jia Xinlei , Huo Hongjun , Li Xueping , Zhang Jiqiang , Zhang Zaiwang , Fang Jidun 
Published: September 2020

AbstractMicrobial fuel cell (MFC) technology is a simple way to accelerate the treatment of the oily sludge which is a major problem affecting the quality of oil fields and surrounding environment while generating electricity. To investigate the oil removal and the characteristics of changes in the composition of bacteria, sediment microbial fuel cells (SMFCs) supplemented with oily sludge was constructed. The results showed that the degradation efficiency of total petroleum hydrocarbon (TPH) of SMFC treatment was 10.1 times higher than the common anaerobic degradation. In addition, the degradation rate of n-alkanes followed the order of high carbon number > low carbon number > medium carbon number. The odd–even alkane predominance (OEP) increased, indicating that a high contribution of even alkanes whose degradation predominates. The OUT number, Shannon index, AEC index, and Chao1 index of the sludge treated with SMFC (YN2) are greater than those of the original sludge (YN1), showing that the microbial diversity of sludge increased after SMFC treatment. After SMFC treatment the relative abundance of Chloroflexi, Bacteroidia and Pseudomonadales which are essential for the degradation of the organic matter and electricity production increased significantly in YN2. These results will play a crucial role in improving the performance of oily sludge MFC.
Ceruloplasmin oxidized and deamidated by Parkinson's disease cerebrospinal fluid induces epithelial cells proliferation arrest and apoptosis
Authors: Barbariga Marco , Zanardi Alan , Curnis Flavio , Conti Antonio , Boselli Daniela , Di Terlizzi Simona , Alessio Massimo 
Published: September 2020

AbstractIn Parkinson's disease, the ferroxidase ceruloplasmin (Cp) is oxidized and deamidated by the pathological cerebrospinal fluid (CSF) environment. These modifications promote the gain of integrin binding properties, fostered by the deamidation of two NGR-motifs present in the Cp sequence that convert into the isoDGR-motif. Through isoDGR/integrin binding, the oxidized/deamidated-Cp (Cp-ox/de) mediates cell adhesion and transduces an intracellular signal in epithelial cells that seems to be addressed to regulate cell cycle, proliferation and cytoskeletal re-arrangement. However, the effect fostered on cells by integrins engagement via Cp-ox/de is not known. We found that in HaCaT epithelial cells, the incubation with Cp-ox/de resulted in proliferation inhibition mediated by isoDGR, cell cycle arrest and apoptosis induction. Similar proliferation inhibition was induced by treatment with purified Cp previously incubated in the CSF from Parkinson's disease patients, but not by Cp incubated in the CSF from healthy subjects. In human primary choroid plexus epithelial cells, a possible in vivo target of Cp-ox/de generated in pathological CSFs, we found that Cp-ox/de mediated cell adhesion via isoDGR/integrins binding and transduced an intracellular signal, which resulted in cell proliferation inhibition. Thus, the generation of Cp-ox/de in pathological CSFs and the consequent apoptosis induction of epithelial cells facing the liquor, might represent a novel mechanism that contributes to neurodegeneration.
Comparison of visual performance between monofocal and multifocal intraocular lenses of the same material and basic design
Authors: Tanabe Hirotaka , Tabuchi Hitoshi , Shojo Tomohiro , Yamauchi Tomofusa , Takase Kosuke 
Published: September 2020

AbstractTo compare the visual performance of a monofocal intraocular lens (IOL) (ZCB00) and a multifocal IOL (ZMB00) of the same material and basic design, we evaluated postoperative parameters at 10 weeks after the last surgery in cataract patients who underwent bilateral ZCB00 or ZMB00 implantation from December 13, 2010, to July 29, 2019, with the right and left lenses implanted within 3 months of each other. The study enrolled 2,230 eyes of 1,115 patients. The monofocal group comprised 904 eyes of 452 patients (72.3 ± 6.8 years; females/males, 268/184), and the multifocal group comprised 1,326 eyes of 663 patients (67.0 ± 7.8 years; females/males, 518/145). Contrast sensitivity (4.0/2.5/1.6/1.0/0.7 degrees), contrast sensitivity with glare (1.6/1.0/0.7 degrees), and the VFQ-25 score for driving at night were significantly better in the monofocal group (p < 0.00068, Wald test). Uncorrected intermediate/near visual acuity and near spectacle independence were significantly better in the multifocal group (p < 0.00068, Wald test). The two IOL groups had different characteristics in terms of contrast sensitivity, night-time driving, uncorrected intermediate/near visual acuity and near spectacle independence.
Nanosecond pulsed electric fields modulate the expression of the astaxanthin biosynthesis genes psy, crtR-b and bkt 1 in Haematococcus pluvialis
Authors: Bai Fan , Gusbeth Christian , Frey Wolfgang , Nick Peter 
Published: September 2020

AbstractNanosecond pulsed electric fields (nsPEFs) have been extensively studied with respect to cellular responses. Whether nsPEFs can regulate gene expression and to modulate the synthesis of valuable compounds, has so far been only tested in the context of apoptosis in cancer cells. We used the unicellular algae Haematococcus pluvialis as system to test, whether nsPEFs could alter gene expression and to promote the biosynthesis of astaxanthin. We find that nsPEFs induce a mild, but significant increase of mortality up to about 20%, accompanied by a moderate increase of astaxanthin accumulation. Steady-state transcript levels of three key genes psy, crtR-b and bkt 1 were seen to increase with a maximum at 3 d after PEF treatment at 50 ns. Pulsing at 25 ns reduce the transcripts of psy, crtR-b from around day 2 after the pulse, while those of bkt 1 remain unchanged. By blocking the membrane-located NADPH oxidase RboH, diphenylene iodonium by itself increased both, the levels of astaxanthin and transcripts of all three biosynthetic genes, and this increase was added up to that produced by nsPEFs. Artificial calcium influx by an ionophore did not induce major changes in the accumulation of astaxanthin, nor in the transcript levels, but amplified the response of crtR-b to nsPEFs at 25 ns, while decreased in 50 ns treatment. When Ca2+ influx was inhibited by GdCl3, the transcript of psy and bkt 1 were decreased for both 25 ns and 50 ns treatments, while crtR-b exhibited an obvious increase for the 25 ns treatment. We interpret these data in a working model, where nsPEFs permeabilise plasma and chloroplast membrane depending on pulse duration leading to a differential release of plastid retrograde signaling to the nucleus.
Complete genome sequence of fish-pathogenic Aeromonas hydrophila HX-3 and a comparative analysis: insights into virulence factors and quorum sensing
Authors: Jin Lei , Chen Yu , Yang Wenge , Qiao Zhaohui , Zhang Xiaojun 
Published: September 2020

AbstractThe gram-negative, aerobic, rod-shaped bacteriumAeromonas hydrophila, the causative agent of motile aeromonad septicaemia, has attracted increasing attention due to its high pathogenicity. Here, we constructed the complete genome sequence of a virulent strain,A. hydrophilaHX-3 isolated fromPseudosciaena croceaand performed comparative genomics to investigate its virulence factors and quorum sensing features in comparison with those of otherAeromonasisolates. HX-3 has a circular chromosome of 4,941,513 bp with a 61.0% G + C content encoding 4483 genes, including 4318 protein-coding genes, and 31 rRNA, 127 tRNA and 7 ncRNA operons. Seventy interspersed repeat and 153 tandem repeat sequences, 7 transposons, 8 clustered regularly interspaced short palindromic repeats, and 39 genomic islands were predicted in theA. hydrophilaHX-3 genome. Phylogeny and pan-genome were also analyzed herein to confirm the evolutionary relationships on the basis of comparisons with other fully sequencedAeromonasgenomes. In addition, the assembled HX-3 genome was successfully annotated against the Cluster of Orthologous Groups of proteins database (76.03%), Gene Ontology database (18.13%), and Kyoto Encyclopedia of Genes and Genome pathway database (59.68%). Two-component regulatory systems in the HX-3 genome and virulence factors profiles through comparative analysis were predicted, providing insights into pathogenicity. A large number of genes related to the AHL-type 1 (ahyI,ahyR), LuxS-type 2 (luxS,pfs,metEHK,litR,luxOQU) and QseBC-type 3 (qseB,qseC) autoinducer systems were also identified. As a result of the expression of theahyIgene inEscherichia coliBL21 (DE3), combined UPLC-MS/MS profiling led to the identification of several new N-acyl-homoserine lactone compounds synthesized by AhyI. This genomic analysis determined the comprehensive QS systems ofA. hydrophila, which might provide novel information regarding the mechanisms of virulence signatures correlated with QS.
Determination of controlled limit value of groundwater level depth and management practice in Xi’an, China
Authors: Mu En-lin , Yan Long , Ding Ai-zhong , Deng Wei , Wang Yong 
Published: September 2020

AbstractBased on the different types of geomorphic units in Xi'an, China, and the groundwater recharge methods of the different geomorphic units, the goal is to ensure the sustainable development and utilization of groundwater, to ensure the natural attributes and to prevent salinization. According to different rainfall conditions, the upper and lower limits of the controlled limit value of groundwater level in different regions are calculated to define the control targets of the different geomorphic units. Based on the calculated controlled limit value of groundwater level and the administrative divisions of Xi'an City, the red lines of groundwater control in each county and city are developed. Four management lines are delineated from the surface to the bottom (from top to bottom, the upper limit of groundwater depth, the upper limit of infiltration of groundwater depth, the lower limit of groundwater depth and the risk line in extremely dry years), and five management areas are delineated (from top to bottom, the prevent soli salinization area, the normal extraction area, the careful extraction area, the permit only in extreme dry years area and the prohibited extraction area) to provide technical support for groundwater management in Xi'an.
A multiple drug loaded, functionalized pH-sensitive nanocarrier as therapeutic and epigenetic modulator for osteosarcoma
Authors: Yuan Ye , Song Jia-Xing , Zhang Mei-Na , Yuan Bao-Shan 
Published: September 2020

AbstractOsteosarcoma is a malignant condition affecting adolescents and children more than adults. Nanobiomedicine has opened up several avenues which have increased therapeutic efficiencies than the conventional treatment for the same. In the current study, a novel organic nanoparticle was devised conjugated with bisphosphonate zoledronic acid which has an affinity for bone tissues. Moreover, the nanoparticle was loaded with multiple anti-cancer drugs like gemcitabine and epirubicin. The nanoparticles were characterized by microscopic analysis, entrapment and loading efficiencies, bone affinity studies, in-vitro release studies, cytotoxicity studies and finally in-vivo tumor regression studies. Bone affinity studies depicted a high affinity of zoledronic acid towards bone powder. The nanoparticle exhibited a nanosize dimension, high entrapment and loading efficiencies with uniform symmetry devoid of agglomeration. The in-vitro release experiments showed a measured release of drugs over a longer time without any hint of burst release. However, the release was comparatively for a longer duration in acidic pH and normal physiological pH which may be excellent for therapeutic efficiency. The cytotoxicity studies revealed enhanced cytotoxic effect for MG-63 cell lines in comparison of free drug or single drug combinations. Nonetheless, they proved to be cytocompatible with primary bone cells. Additionally, cellular uptake of nanoparticle was appreciably improved. Significant tumor (250%) regression was seen upon treatment with multiple drug loaded zoledronic acid conjugated nanoparticle, along with epigenetic changes affecting microRNA expressions. The increased cytotoxicity and increased cellular uptake may be of greater advantage in systemic osteosarcoma therapy. Combining all results, our study demonstrated substantial potential towards management of osteosarcoma.
Landscape of immune checkpoint inhibitor-related adverse events in Chinese population
Authors: Li Li , Li Gang , Rao Bin , Dong An-Hui , Liang Wei , Zhu Jin-Xian , Qin Mu-Ping , Huang Wen-Wen , Lu Jie-Ming , Li Zi-Fang , Wu Yao-Zhong 
Published: September 2020

AbstractThis study aimed to describe the landscape of Immune checkpoint inhibitors (ICIs)-related adverse events (AEs) in a predominantly Chinese cohort. We searched electronic datasets including PubMed, Web of Science and Embase to identify and recruit relevant trials up to September 2, 2019. Clinical trials focusing on ICIs in Chinese patients or a predominantly Chinese population were included. Incidences of treatment-related AEs (TRAEs) and immune-related AEs (irAEs) were pooled and compared. In total, we recruited 13 trials consisting of 1063 patients, with 922 (86.7%) receiving ICI monotherapy and 141 (13.3%) receiving combination of ICI with chemotherapy or anti-angiogenesis. The pooled incidence of any grade TRAEs, grade 1–2, grade 3–5 TRAEs, any grade irAEs, grade 1–2 irAEs and grade 3–5 irAEs in all 1063 patients were 84.1%, 63.3%, 20.9%, 43.3%, 40.0% and 3.0%, respectively. Moreover, 4.3% (44/1018) of patients experienced treatment discontinuation and only 8 (0.8%) patients experienced treatment-related death. Compared to ICI monotherapy, combination significantly increased grade 3–5 TRAEs (46.1% vs. 17.0%, P < 0.001) and grade 3–5 irAEs (7.1% vs. 2.0%, P = 0.015). By comparing the toxicity profiles between different ICIs, we found some drug-specific AEs such as reactive capillary haemangiomas for camrelizumab (58.6%), hyperglycemia for toripalimab (55.6%) and pyrexia for tislelizumab (54.3%). Additionally, nivolumab has the lowest incidence of any grade (64.1%) and grade 3–5 (11.8%) TRAEs. ICI-related AEs were generally mild and tolerable for a predominantly Chinese cohort. However, we should pay attention to the combination of ICI with chemotherapy as it could increase grade 3–5 TRAEs and irAEs.
Advanced risk-based event attribution for heavy regional rainfall events
Authors: Imada Yukiko , Kawase Hiroaki , Watanabe Masahiro , Arai Miki , Shiogama Hideo , Takayabu Izuru 
Published: September 2020

AbstractRisk-based event attribution (EA) science involves probabilistically estimating alterations of the likelihoods of particular weather events, such as heat waves and heavy rainfall, owing to global warming, and has been considered as an effective approach with regard to climate change adaptation. However, risk-based EA for heavy rain events remains challenging because, unlike extreme temperature events, which often have a scale of thousands of kilometres, heavy rainfall occurrences depend on mesoscale rainfall systems and regional geographies that cannot be resolved using general circulation models (GCMs) that are currently employed for risk-based EA. Herein, we use GCM large-ensemble simulations and high-resolution downscaled products with a 20-km non-hydrostatic regional climate model (RCM), whose boundary conditions are obtained from all available GCM ensemble simulations, to show that anthropogenic warming increased the risk of two record-breaking regional heavy rainfall events in 2017 and 2018 over western Japan. The events are examined from the perspective of rainfall statistics simulated by the RCM and from the perspective of background large-scale circulation fields simulated by the GCM. In the 2017 case, precipitous terrain and a static pressure pattern in the synoptic field helped reduce uncertainty in the dynamical components, whereas in the 2018 case, a static pressure pattern in the synoptic field provided favourable conditions for event occurrence through a moisture increase under warmer climate. These findings show that successful risk-based EA for regional extreme rainfall relies on the degree to which uncertainty induced by the dynamic components is reduced by background conditioning.
Long-term outcomes of therapist-guided Internet-delivered cognitive behavior therapy for pediatric obsessive-compulsive disorder
Authors: Lenhard Fabian , Andersson Erik , Mataix-Cols David , Rück Christian , Aspvall Kristina , Serlachius Eva 
Published: September 2020

AbstractCognitive behavior therapy (CBT) is the recommended first-line intervention for children and adolescents with obsessive-compulsive disorder (OCD), but is not broadly accessible. Internet-delivered CBT (ICBT) with minimal therapist support is efficacious and cost-effective, at least in the short term. Whether the therapeutic gains of ICBT for OCD are sustained in the long run is unknown. In this study, 61 adolescents with OCD who participated in a randomized trial of ICBT were followed-up 3 and 12 months after treatment. The proportion of treatment responders and remitters remained stable from post-treatment to 3-month follow-up and increased significantly from 3-month to 12-month follow-up. This study suggests that the gains of ICBT for youth with OCD are not only maintained long-term, but that further improvements continue to occur during follow-up.