bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro ketone‐supported mitochondrial respiration is minimal when other substrates are readily available in cardiac and skeletal muscle
Authors: Petrick Heather L. , Brunetta Henver S. , Pignanelli Chris , Nunes Everson A. , van Loon Luc J. C. , Burr Jamie F. , Holloway Graham P. 
Published: July 2020

Key points
Ketone bodies are proposed to represent an alternative fuel source driving energy production, particularly during exercise.
Biologically, the extent to which mitochondria utilize ketone bodies compared to other substrates remains unknown.
We demonstrate in vitro that maximal mitochondrial respiration supported by ketone bodies is low when compared to carbohydrate‐derived substrates in the left ventricle and red gastrocnemius muscle from rodents, and in human skeletal muscle.
When considering intramuscular concentrations of ketone bodies and the presence of other carbohydrate and lipid substrates, biological rates of mitochondrial respiration supported by ketone bodies are predicted to be minimal.
At the mitochondrial level, it is therefore unlikely that ketone bodies are an important source for energy production in cardiac and skeletal muscle, particularly when other substrates are readily available.
AbstractKetone bodies (KB) have recently gained popularity as an alternative fuel source to support mitochondrial oxidative phosphorylation and enhance exercise performance. However, given the low activity of ketolytic enzymes and potential inhibition from carbohydrate oxidation, it remains unknown if KBs can contribute to energy production. We therefore determined the ability of KBs (sodium dl‐β‐hydroxybutyrate, β‐HB; lithium acetoacetate, AcAc) to stimulate in vitro mitochondrial respiration in the left ventricle (LV) and red gastrocnemius (RG) of rats, and in human vastus lateralis. Compared to pyruvate, the ability of KBs to maximally drive respiration was low in isolated mitochondria and permeabilized fibres (PmFb) from the LV (∼30–35% of pyruvate), RG (∼10–30%), and human vastus lateralis (∼2–10%). In PmFb, the concentration of KBs required to half‐maximally drive respiration (LV: 889 µm β‐HB, 801 µm AcAc; RG: 782 µm β‐HB, 267 µm AcAc) were greater than KB content representative of the muscle microenvironment (∼100 µm). This would predict low rates (∼1–4% of pyruvate) of biological KB‐supported respiration in the LV (8–14 pmol s−1 mg−1) and RG (3–6 pmol s−1 mg−1) at rest and following exercise. Moreover, KBs did not increase respiration in the presence of saturating pyruvate, submaximal pyruvate (100 µm) reduced the ability of physiological β‐HB to drive respiration, and addition of other intracellular substrates (succinate + palmitoylcarnitine) decreased maximal KB‐supported respiration. As a result, product inhibition is likely to limit KB oxidation. Altogether, the ability of KBs to drive mitochondrial respiration is minimal and they are likely to be outcompeted by other substrates, compromising their use as an important energy source.
Three-Dimensional Simulation of Highly Unsteady and Isothermal Flow in Centrifugal Pumps for the Local Loss Analysis Including a Wall Function for Entropy Production
Authors: Melzer Steffen , Pesch Andreas , Schepeler Stephan , Kalkkuhl Tobias , Skoda Romuald 
Published: July 2020

Abstract
A local loss analysis (LLA) based on entropy production is presented for the numerical three-dimensional (3D) simulation of isothermal centrifugal pump flow. A finite volume method and a statistical turbulence model are employed. Wall functions for direct and turbulent entropy production in isothermal flow are derived, implemented in a node-centered finite volume scheme as a postprocessing procedure, and validated on an attached channel flow as well as on separated flow in an asymmetric diffuser. The integrity of the entropy wall function is demonstrated by a loss balance for a wide range of boundary layer resolution in terms of nondimensional wall distance y+≈1 to ≈200. Remaining differences to the total pressure loss are traced back to the particular turbulent wall function for the flow solution within the finite volume solver and vanish toward a wall resolution of the viscous sublayer, i.e., y+≈1. LLA together with the new entropy wall function is applied to highly unsteady isothermal flow in a single-blade pump as well as to part-load operation of a conventional multiblade pump which reveals distinctive flow structures that are associated with entropy production. By these examples, it is demonstrated how efficiency characteristics of centrifugal pumps can be attributed to local loss production in particular flow regions.
Effects of 2 Methods of Combined Training on Cardiometabolic Risk Factors in Adolescents: A Randomized Controlled Trial
Authors: Faria Waynne F. , Mendonça Filipe R. , Santos Géssika C. , Kennedy Sarah G. , Elias Rui G.M. , Stabelini Neto Antonio 
Published: July 2020

Purpose: To analyze the effects of 2 combined training methods on the cardiometabolic risk factors in adolescents. Methods: A total of 76 adolescents (16.1 [1.1] y, n = 44 female) were randomized into groups of moderate-intensity continuous training combined with resistance training (MICT + RT), high-intensity interval training combined with resistance training (HIIT + RT), or control. The training sessions were performed twice weekly for 12 weeks. The outcomes evaluated included body fat percentage, waist circumference, fasting blood glucose, fasting insulin, total cholesterol, triglycerides, low-density lipoprotein, high-density lipoprotein, glycated hemoglobin, insulin resistance index, blood pressure, peak oxygen consumption (VO2peak), and cardiometabolic risk Z score. Results: The intervention groups presented a significant reduction in the cardiometabolic risk Z score after 12 weeks of the combined exercise program. In relation to the cardiometabolic risk Z score between groups, the HIIT + RT group presented a significant intervention effect when compared with the control group (Cohen d = 0.23; P < .05). Significant intervention effects were found when comparing the MICT + RT and control groups for body fat percentage, high-density lipoprotein, and VO2peak. Between the HIIT + RT and control groups, significant intervention effects were found for body fat percentage, blood pressure, and VO2peak. There were not significant differences between the HIIT + RT and MICT + RT groups. Conclusion: Twelve weeks of HIIT + RT and MICT + RT were effective in significantly reducing the cardiometabolic risk in these adolescents.
Oxidative conditions can lead to exceptional preservation through phosphatization
Authors: Gueriau Pierre , Bernard Sylvain , Farges François , Mocuta Cristian , Dutheil Didier B. , Adatte Thierry , Bomou Brahimsamba , Godet Marie , Thiaudière Dominique , Charbonnier Sylvain , Bertrand Loïc 
Published: July 2020

Abstract
Exceptional preservation through phosphatization is primarily controlled by a reduction in pH, favoring the precipitation of apatite over that of calcite. Laboratory experiments have suggested that phosphatization results from anoxic decay. Here we report results of the fine-scale mineralogical characterization of Cretaceous phosphatized fossils of teleost fishes and crustaceans from the Jebel oum Tkout Lagerstätte (Morocco). Data collected using complementary laboratory and synchrotron-based X-ray techniques reveal that oxidative conditions were established at a certain step of decay. Supporting these conclusions are the presence, covering and embedded in the phosphatized tissues, of Fe(III)-rich mineral phases, the precipitation of which was likely biologically induced during decay. The present study highlights that the establishment of oxidative conditions during decay can be compatible with exceptional preservation of fossils through phosphatization.
Identifying the facilitators, constraints and barriers of community indoor walking programmes: protocol for a realist synthesis
Authors: Singh Shaminder , Yang Lin , Butalia Sonia , Quan Hude , Turin Tanvir C 
Published: July 2020

IntroductionPhysical inactivity is a costly and leading health risk factor. Engaging in moderate or more intense regular physical activity reduces premature mortality at the population level. Walking is a viable option for achieving the recommended level of physical activity. Yet, the sedentary lifestyle is trending. Determinants of physical activity may be personal, social or environmental. Health promotion endeavours aiming to enhance population-level physical activity are reported in the literature. However, a full range of factors influencing the development and implementation of sustainable indoor walking programmes is unclear. The current review protocol is aimed at describing a process of realist synthesis to uncover contexts, mechanisms and outcomes of indoor walking intervention programmes, which might reveal facilitators, constraints and barriers of planning, implementing and participating in indoor walking initiatives open for the members of the general public.Methods and analysisWe will employ a realist synthesis to determine successes or failures in certain circumstances for specific stakeholders, which will aid in developing a sustainable mall walking health promotion and community engagement programme. Qualitative, quantitative and mixed-method articles and reports will be screened for intervention theories and models in order to identify elements of programmes that may be linked to the success or failure of the interventions. Data related to the context, mechanism and outcome of the interventions will be collected, analysed and synthesised iteratively until a theoretical understanding develops, which might explain the intricacies of the success and failure of identified indoor walking programmes. The review process will be conducted and evaluated by using the recommended tools.Ethics and disseminationEthical approval, such as Conjoint Health Research Ethics Board, was not required for this study because no direct interaction with patients will occur for data collection and analysis. We will disseminate directly to the scholarly community through publication and presentation and may post on social media or websites.PROSPERO registration numberCRD42020150415.
Primary medication non-adherence at Counties Manukau Health Emergency Department (CMH-ED), New Zealand: an observational study
Authors: Martini Nataly Dominica , van der Werf Bert , Bassett-Clarke Deborah 
Published: July 2020

ObjectivesTo measure primary medication non-adherence (failure to fill prescription medicines) in patients discharged from the emergency department (ED), and to determine whether sociodemographic factors, smoking status and access to a general practitioner affect prescription filling. Little is known about primary medication non-adherence in EDs, and less so in New Zealand (NZ). Identifying reasons for non-adherence will enable development of strategies to improve adherence and reduce morbimortality.Design and settingAn observational study based on patient data from the ED of a large public hospital in South Auckland, NZ.ParticipantsData were collected from 1600 patients discharged between 28 April–6 May and 28 July–9 August 2014. Data were included if patients were residents within the Auckland Regional Public Health Service boundaries, admitted to ED and discharged with a prescription. Data were excluded if patients were admitted to another ward, transferred to another hospital or left the ED without seeing a doctor.Results992 patients were included in the study, the majority were under 10 years (32.6%), of Pacific Island descent (42.8%), NZ-born (67.7%) and living in the most socioeconomically deprived areas (78.1%). Almost 50% of patients failed to fill all prescription medications. Simple linear regression analysis indicated that non-adherence was significant for those 10–24 years (n=236; adherence=47.2%; p<0.05), of NZ Māori ethnicity (n=175; 51.3%; p=0.01), unemployed (n=77; 46.8%; p<0.01), homemakers (n=66; 45.7%; p<0.01), students (n=228; 55.6%; p<0.05) and cigarette smokers (n=139; 50.3%; p<0.01). Following multivariable analysis, the strongest predictors for non-adherence were those aged between 10 and 17 years (n=116; p<0.01), the unemployed (n=77; p=0.01) and homemakers (n=66; p=0.01).ConclusionsAge and occupation were the greater predictors of non-adherence; however, no other significant differences were found. Since this study, changes to prescription co-payments have been made. Further research is warranted to assess whether this change has more recently affected the rates of non-adherence.
Receipt of routine preventive care among infant daughters and sons of immigrant mothers in Ontario, Canada: a retrospective cohort study
Authors: Pulver Ariel , Guttmann Astrid , Ray Joel G , O'Campo Patricia J , Urquia Marcelo L 
Published: July 2020

ObjectivesTo explore gender disparities in infant routine preventive care across maternal countries of birth (MCOB) and by mother tongue among infants of Indian-born mothers.SettingRetrospective population-based administrative cohort in Ontario, Canada (births between 2002 and 2014).Participants350 366 (inclusive) healthy term singletons belonging to families with a minimum of one opposite gender child.Outcome measuresFixed effects conditional logistic regression generated adjusted ORs (aORs) for a daughter being underimmunised and having an inadequate number of well-child visits compared with her brother, stratified by MCOB. Moderation by maternal mother tongue was assessed among children to Indian-born mothers.ResultsUnderimmunisation and inadequate well-child visits were common among both boys and girls, ranging from 26.5% to 58.2% (underimmunisation) and 10.5% to 47.8% (inadequate well-child visits). depending on the maternal birthplace. Girls whose mothers were born in India had 1.19 times (95% CI 1.07 to 1.33) the adjusted odds of inadequate well-child visits versus their brothers. This association was only observed among the Punjabi mother tongue subgroup (aOR: 1.26, 95% CI 1.08 to 1.47). In the Hindi mother tongue subgroup, girls had lower odds of underimmunisation than their brothers (aOR: 0.73, 95% CI 0.54 to 0.98).ConclusionsGender equity in routine preventive healthcare is mostly achieved among children of immigrants. However, daughters of Indian-born mothers whose mother tongue is Punjabi, appear to be at a disadvantage for well-child visits compared with their brothers. This suggests son preference may persist beyond the family planning stage among some Indian immigrants.
Favipiravir for treating patients with novel coronavirus (COVID-19): protocol for a systematic review and meta-analysis of randomised clinical trials
Authors: Arab-Zozani Morteza , Hassanipour Soheil , Ghoddoosi-Nejad Djavad 
Published: July 2020

IntroductionAn outbreak of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was reported in Wuhan, China, in mid-December 2019, and declared a pandemic by the WHO on 11 March 2020. Due to the unknown nature of the disease and the lack of specific drugs, several potential treatments were used for patients. This systematic review and meta-analysis will evaluate studies of the effects of favipiravir in COVID-19 pneumonia.Methods and analysisWe will search electronic databases including LitCovid hub, PubMed, Scopus, ISI Web of Sciences, Cochrane and Embase using keywords related to COVID-19 and favipiravir. We will search the reference lists of all included studies and reviews. We will also search for clinical trial registries, such as ClinicalTrials.gov, for the ongoing clinical trials. All randomised clinical trials investigating the safety and efficacy of favipiravir compared with other control groups for the treatment of patients with confirmed infection with SARS-CoV-2 will be included. Patients’ survival at the end of the treatment as well as the follow-up will be the primary outcome of the treatment, followed by the time and rate of the patient with a negative COVID-19 test. The desired secondary outcome will consist of a decreased rate of symptoms, proportion of intensive care unit (ICU) transfers, length of the hospital stay, ICU treatments, the quality of life and additional adverse events. Data synthesis will be conducted using CMA V.2. Two independent investigators will be screening titles, abstracts and full texts of included studies, based on eligibility criteria. These investigators will then independently extract the data and appraise the quality of said studies. All potential discrepancies will be resolved through consultation with the third reviewer. Statistical heterogeneity will be assessed using a standard I2 test. A funnel plot, Egger’s test and Begg’s test will be used for detecting asymmetry to explore possible publication bias.Ethics and disseminationAll findings of this systematic review and meta-analysis will help identify the safety and efficacy of favipiravir for patients with COVID-19. Given that the design of the study is a systematic review, there is no need to follow the code of ethics protocol. The results of this study will be published in a reputable journal.PROSPERO registration numberCRD42020180032.
Sudden cardiac death in asymptomatic patients with aortic stenosis
Authors: Minners Jan , Rossebo Anne , Chambers John B , Gohlke-Baerwolf Christa , Neumann Franz-Josef , Wachtell Kristian , Jander Nikolaus 
Published: July 2020

ObjectiveWe retrospectively analysed outcome data from the Simvastatin and Ezetimibe in Aortic Stenosis (SEAS) study to assess the incidence and potential risk factors of sudden cardiac death (SCD) in this prospectively followed cohort of asymptomatic patients with aortic stenosis (AS).MethodsOf the 1873 patients included in SEAS, 1849 (99%) with mild to moderate AS (jet velocity 2.5–4.0 m/s at baseline) and available clinical, echocardiographic and follow-up data were analysed. Patients undergoing aortic valve replacement were censored at the time of operation.ResultsDuring an overall follow-up of 46.1±14.6 months, SCD occurred in 27 asymptomatic patients (1.5%) after a mean of 28.3±16.6 months. The annualised event rate was 0.39%/year. The last follow-up echocardiography prior to the event showed mild to moderate stenosis in 22 and severe stenosis (jet velocity >4 m/s) in 5 victims of SCD. The annualised event rate after the diagnosis of severe stenosis was 0.60%/year compared with 0.46%/year in patients who did not progress to severe stenosis (p=0.79). Patients with SCD were older (p=0.01), had a higher left ventricular mass index (LVMI, p=0.001) and had a lower body mass index (BMI, p=0.02) compared with patients surviving follow-up. Cox regression analysis identified age (HR 1.06, 95% CI 1.01 to 1.11 per year, p=0.02), increased LVMI (HR 1.20, 95% CI 1.10 to 1.32 per 10 g/m2, p<0.001) and lower BMI (HR 0.87, 95% CI 0.79 to 0.97 per kg/m2, p=0.01) as independent risk factors of SCD.ConclusionSCD in patients with asymptomatic mild to moderate AS is rare and strongly related to left ventricular hypertrophy but not stenosis severity.
Need for consistent beach lifeguard data collection: results from an international survey
Authors: Koon William , Schmidt Andrew , Queiroga Ana Catarina , Sempsrott Justin , Szpilman David , Webber Jonathon , Brander Robert 
Published: July 2020

BackgroundLifeguards are integral to beach safety and collect data which is used for a variety of purposes, although guidelines and best practice have yet to be established. This study served to identify and characterise existing beach lifeguard service provider (BLSP) data collection procedures in order to identify the degree of uniformity and areas for improvement.MethodsThe ‘International Beach Lifeguard Data Collection and Reporting’ online survey was distributed via the International Drowning Researchers’ Alliance to BLSP supervisors and managers. The survey included questions on beach conditions and lifeguard activity data collection practices, and respondent’s opinions on their own BLSP’s methods.ResultsVariability in data collection practices was evident in surveys obtained from 55 lifeguard leaders in 12 countries. Discrepancies exist in definitions for ‘rescue’ among BLSPs, a significant amount of information related to beach conditions are recorded and beach visitation is primarily obtained by visual estimate. Respondents expressed challenges with getting frontline staff to collect information in the field and ensuring reporting consistency between recorders. They identified rescue victim demographic factors as key data they would like to collect in the future.ConclusionsInconsistencies in lifeguard data collection present challenges to operations, safety education and prevention efforts, research and policy relying on these data. Variation in definitions, methods and collected variables generally restricts analysis to a single BLSP with limited generalisability to other beach settings. Some gaps in lifeguard data collection may soon be addressed by technology, but developing uniform, internationally acceptable standards and definitions should be prioritised.
Hyperdense vessel sign as a potential guide for the choice of stent retriever versus contact aspiration as first-line thrombectomy strategy
Authors: Mohammaden Mahmoud H , Haussen Diogo C , Perry da Camara Catarina , Pisani Leonardo , Olive Gadea Marta , Al-Bayati Alhamza R , Liberato Bernardo , Rangaraju Srikant , Frankel Michael R , Nogueira Raul G 
Published: July 2020

BackgroundThe first-pass effect (FPE) has emerged as a key metric for efficacy in mechanical thrombectomy (MT). The hyperdense vessel sign (HDVS) on non-contrast head CT (NCCT) indicates a higher clot content of red blood cells.ObjectiveTo assess whether the HDVS could serve as an imaging biomarker for guiding first-line device selection in MT.MethodsA prospective MT database was reviewed for consecutive patients with anterior circulation large vessel occlusion stroke who underwent thrombectomy with stent retriever (SR) or contact aspiration (CA) as first-line therapy between January 2012 and November 2018. Pretreatment NCCT scans were evaluated for the presence of HDVS. The primary outcome was FPE (modified Thrombolysis in Cerebral Infarction score 2c/3). The primary analysis was the interaction between HDVS and thrombectomy modality on FPE. Secondary analyses aimed to evaluate the predictors of FPE.ResultsA total of 779 patients qualified for the analysis. HDVS and FPE were reported in 473 (60.7%) and 286 (36.7%) patients, respectively. The presence of HDVS significantly modified the effect of thrombectomy modality on FPE (p=0.01), with patients with HDVS having a significantly higher rate of FPE with a SR (41.3% vs 22.2%, p=0.001; adjusted OR 2.11 (95% CI 1.20 to 3.70), p=0.009) and non-HDVS patients having a numerically better response to CA (41.4% vs 33.9%, p=0.28; adjusted OR 0.58 (95% CI 0.311 to 1.084), p=0.088). Age (OR 1.01 (95% CI 1.00 to 1.02), p=0.04) and balloon guide catheter (OR 2.08 (95% CI 1.24 to 3.47), p=0.005) were independent predictors of FPE in the overall population.ConclusionOur data suggest that patients with HDVS may have a better response to SRs than CA for the FPE. Larger confirmatory prospective studies are warranted.