bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lost and lonely: a qualitative study of women’s experiences of no embryo transfer owing to non-fertilization or poor embryo quality
Authors: Holter Herborg , Bergh Christina , Gejervall Ann-Louise 
Published: November 2020

Abstract

STUDY QUESTION
What are the experiences of women undergoing IVF who could not receive an embryo transfer because of failed fertilization or poor embryo development?

SUMMARY ANSWER
No embryo transfer because of failed embryo development is associated with considerable emotional suffering and the need for an early appointment with a physician to obtain information on what went wrong and new alternatives.

WHAT IS KNOWN ALREADY
The psychological and emotional impact of IVF treatments as experienced by IVF patients is well known, particularly following the failure to achieve pregnancy.

STUDY DESIGN, SIZE, DURATION
A qualitative study running from January 2018 to April 2019 was carried out at one public and one private IVF clinic. The invitation to participate was sent to women within 1 month after the cycle failed.

PARTICIPANTS/MATERIALS, SETTING, METHODS
The women undergoing IVF were diverse in terms of cause of infertility, age, number of previous cycles, country of birth and educational level. Nineteen of the 41 invited women who had experienced no embryo transfer because of non-fertilization or poor embryo development took part in a semi-structured interview. Data were analysed by thematic content analysis.

MAIN RESULTS AND THE ROLE OF CHANCE
The master theme was identified as: ‘Lost and lonely’ organized in two main themes ‘Experience of the event’ and ‘Perception of needs from healthcare providers’. Considerable emotional suffering was recognized after no embryo transfer. The need for support was expressed as to be offered an early appointment with a physician for information about what went wrong, looking at new alternatives and, for many women, providing information about counselling.

LIMITATIONS, REASONS FOR CAUTION
Only women participated, not partners. Of the women invited, 46% participated. Several declined to participate because of high levels of emotional stress.

WIDER IMPLICATIONS OF THE FINDINGS
Patients undergoing IVF and not achieving embryo transfer due to poor embryo development are a vulnerable group. They need early feedback concerning reasons for failure and future alternatives. They also require psychological support. This ought to be offered by IVF clinics.

STUDY FUNDING/COMPETING INTEREST(S)
The study was financed by grants from the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement (ALFGBG-70940). It was also financed by the Local Research and Development Board for Gothenburg and Södra Bohuslän (VGFOUGSB-866771) and by Hjalmar Svensson’s research foundation (HJSV-2017012). None of the authors declares any conflicts of interest.

LARGE SCALE DATA
N/A.
Analysis of the Illicit Opioid U-48800 and Related Compounds by LC–MS-MS and Case Series of Fatalities Involving U-48800
Authors: Fogarty Melissa F , Mohr Amanda L A , Papsun Donna M , Logan Barry K 
Published: November 2020

Abstract
We report a method for the detection and quantitation of 12 drugs and 2 metabolites in the same structural class as the illicit mu-opioid agonist U-47700 in human whole blood. These substances are either known or suspected to be present as potential novel opioids in illicit drug markets. The general class of these drugs was developed in pharmaceutical research programs in the 1970s, but these drugs have recently become of concern for overdoses and death in opioid users in the USA and internationally. The scope of analysis included the following compounds: methylenedioxy U-47700, ethylenedioxy U-47700, ethylenedioxy U-51754, U-69593, U-47931E (bromadoline), U-47700, U-48800, U-49900, U-51754, U-50488, propyl U-47700 and isopropyl U-47700. Additionally, two metabolites N,N-didesmethyl U-47700 and desmethyl U-47700 were also included in the scope. Drugs were extracted from human whole blood using solid-phase extraction, and the extracts were analyzed by liquid chromatography--tandem mass spectrometry. The assay was validated with respect to bias, carryover, interference, within-run and between-run precision, and accuracy. Eight medicolegal death investigation cases that had screened positive for U-48800 by liquid chromatography--time-of-flight mass spectrometry were successfully confirmed and quantified using this method. The mean and median concentrations of U-48800 in these cases were 2.5 (±2.1) and 1.8 ng/mL, respectively, with a range of concentrations of 0.27–6.2 ng/mL. Case history information including the presence of other drugs in combination are described and discussed.
Monitoring the Resistance of the Beet Armyworm (Lepidoptera: Noctuidae) to Four Insecticides in Southern China from 2014 to 2018
Authors: Wang Pan , Yang Fan , Wang Yong , Zhou Li-Lin , Luo Hai-Bo , Zhang Shuai , Si Sheng-Yun 
Published: November 2020

Abstract
The beet armyworm Spodoptera exigua (Hübner) is a serious polyphagous pest that infests vegetable crops worldwide and has rapidly developed resistance due to its long-term exposure to insecticides. The current resistance statuses to four insecticides exhibited by three field populations of beet armyworms collected in southern China from 2014 to 2018 were investigated. Monitoring data from five consecutive years demonstrated that all three tested S. exigua populations developed extremely high resistance to chlorantraniliprole in 2018 (220.58- to 2,597.39-fold). Two populations (Baiyun and Fengxian) developed low to moderate resistance to spinosad, whereas the Huangpi population remained susceptible (except in 2014, with RR of 6.11-fold). The RR of the Fengxian and Baiyun populations to indoxacarb steadily increased over the years, whereas that of the Huangpi population increased relatively slowly. The Baiyun and Fengxian populations developed moderate to high resistance to indoxacarb and methoxyfenozide, whereas the Huangpi population exhibited susceptibility to low resistance (1.06- to 6.45-fold) to indoxacarb and susceptibility to moderate resistance (1.53- to 14.22-fold) to methoxyfenozide. These results suggest that chlorantraniliprole should not be employed to control this pest in southern China. Reduced use of indoxacarb and methoxyfenozide or the use of alternating insecticides with low levels of resistance is recommended. Spinosad remains an effective insecticide for the management of S. exigua. To avoid the rapid development of insecticide resistance, rotations of insecticides with low levels of resistance and different modes of action based on the resistance patterns of S. exigua should be performed in southern China.
Tolerance in Maize Landraces toDiabrotica speciosa(Coleoptera: Chrysomelidae) Larvae and Its Relationship to Plant Pigments, Compatible Osmolytes, and Vigor
Authors: Costa Eduardo Neves , Sardinha de Souza Bruno Henrique , Ribeiro Zulene Antônio , dos Santos Durvalina Maria Mathias , Boiça Arlindo Leal 
Published: November 2020

AbstractThe rootworm Diabrotica speciosa (Germar) is native to South America and causes severe economic losses to several crops due to root feeding and disease spread. In maize (Zea mays L.), losses in production come from larval rootworm attack on plant roots resulting in plant health problems, including stalk lodging. More options for controlling this pest are needed to create well balanced, integrated pest management programs for farmers in this region. Natural sources of tolerance in maize genotypes are important for maize breeding programs, and this study investigated the expression of tolerance in several Brazilian maize landraces to D. speciosa. Plant vigor and compounds associated with plant health, including chlorophylls, carotenoids, glycine betaine, and proline were assessed for each landrace. Five landraces and one maize cultivar were selected based on their levels of antibiosis-resistance to D. speciosa that were determined in a prior screening. The percent reduction in plant growth was used as the measure of tolerance. The landrace Azteca was classified as tolerant to D. speciosa larval feeding, displaying less reduction in plant matter despite having lower plant vigor. This landrace also had higher amounts of chlorophyl and carotenoid pigments, suggesting a positive correlation between tolerance to D. speciosa and higher contents of these photosynthetic pigments. The compatible osmolytes glycine betaine and proline do not seem to be associated with tolerance in maize landraces to D. speciosa larvae. Landrace Azteca seems promising for plant breeding, and repeated field studies are needed to confirm its suitability in maize integrated pest management.
The International Criminal Court versus the African Criminal Court
Authors: Martini Pauline 
Published: November 2020

Abstract
In 2014, the Assembly of the African Union amended the Statute of the prospective African Court of Justice and Human Rights (ACJHR) by the adoption of the Malabo Protocol. The Annex to the Protocol confers an extensive international criminal jurisdiction to the ACJHR’s International Criminal Law Section — often referred to as the African Criminal Court — which overlaps with the jurisdiction of the International Criminal Court (ICC). If the Protocol enters into force, the Courts will exercise concurrent jurisdiction over the same matters. Conflicts of jurisdiction would therefore arise should both Courts decide to exercise jurisdiction over the same situation or case. This article advocates for a remodelling of the principle of complementarity, enshrined in Article 17 of the Rome Statute and in Article 46H of the Annex to the Malabo Protocol, to solve these potential conflicts of jurisdiction. The arguments presented herein will be relevant to the relationship between the ICC and any regional criminal court which prosecutes international crimes that would take shape.
Fleas (Siphonaptera) Parasitizing Peridomestic and Indigenous Mammals in Panamá and Screening of Selected Fleas for Vector-Borne Bacterial Pathogens
Authors: Durden Lance A , Bermúdez Sergio , Vargas Géminis A , Sanjur Boris E , Gillen Laura , Brown Lisa D , Greiman Stephen E , Eremeeva Marina E 
Published: November 2020

AbstractIn total, 341 fleas belonging to 16 species were collected from 78 host mammals belonging to 10 species in Panamá from 2010 to 2016. The cat flea, Ctenocephalides felis (Bouché) predominated on domestic dogs and was also recorded from domestic cats, the raccoon, Procyon lotor (Linnaeus) and the common opossum, Didelphis marsupialis Linnaeus. The largest number of flea species (7) was recorded from D. marsupialis and the most common flea on that host was the ctenophthalmid, Adoratopsylla intermedia copha Jordan. One Oriental rat flea, Xenopsylla cheopis (Rothschild), was collected from D. marsupialis. Native rodents were parasitized by indigenous ceratophyllid, rhopalopsyllid, and stephanocircid fleas. The Mexican deermouse, Peromyscus mexicanus (Saussure), was parasitized by six species of ceratophyllids belonging to the mostly Central American genera, Baculomeris, Jellisonia, Kohlsia and Plusaetis. The long-tailed singing mouse, Scotinomys xerampelinus (Bangs), was parasitized by Plocopsylla scotinomi Tipton and Méndez, the only species of stephanocircid flea known from Central America. Twenty-six pools of extracted flea DNA representing 5 flea species (C. felis, Pulex echidnophagoides (Wagner), Pulex simulans Baker, A. intermedia copha, and P. scotinomi) and 79 individual fleas were all real-time polymerase chain reaction negative for Rickettsia felis, Rickettsia typhi, and Bartonella henselae.
Silencing of lncRNA XLOC_035088 Protects Middle Cerebral Artery Occlusion-Induced Ischemic Stroke by Notch1 Signaling
Authors: Chen Miao , Wang Feng , Wang Hairong 
Published: November 2020

Abstract
Ischemic stroke represents one of the leading causes of mortality worldwide and especially in developing countries. It is crucial for finding effective therapeutic targets that protect the brain against ischemic injury. Long noncoding RNAs (lncRNAs) have emerged as major regulators of neurological diseases, and clarifying their roles in cerebral ischemic injury may provide novel targets for the treatment of ischemic stroke. We aimed to investigate the role of lncRNA-XLOC_035088 in middle cerebral artery occlusion (MCAO)-induced rat brain injury and oxygen-glucose deprivation (OGD)-reperfusion treated hippocampal neurons. In our findings, we found that XLOC_035088 expression was significantly upregulated in OGD-reperfusion treated hippocampal neurons and in different brain regions of MCAO-treated rats. XLOC_035088 silencing protected against MCAO-induced ischemic brain injury in vivo and OGD-induced hippocampal neuronal apoptosis in vitro. Intrahippocampal silencing of XLOC_035088 significantly decreased brain XLOC_035088 expression, reduced brain infarct size, and improved neurological function through inhibiting NOTCH1 following derepression of presenilin 2 (PSEN2). Taken together, this study provides evidence that the lncRNA XLOC_035088/PSEN2/Notch1 axis is involved in the pathogenesis of ischemic brain injury, and presents a promising therapeutic route for ischemic stroke.
Losing oceans: The effects of composition on the thermal component of impact-driven atmospheric loss
Authors: Biersteker John B , Schlichting Hilke E 
Published: November 2020

ABSTRACT
The formation of the Solar system’s terrestrial planets concluded with a period of giant impacts. Previous works examining the volatile loss caused by the impact shock in the moon-forming impact find atmospheric losses of at most 20–30 per cent and essentially no loss of oceans. However, giant impacts also result in thermal heating, which can lead to significant atmospheric escape via a Parker-type wind. Here we show that H2O and other high-mean molecular weight outgassed species can be efficiently lost through this thermal wind if present in a hydrogen-dominated atmosphere, substantially altering the final volatile inventory of terrestrial planets. We demonstrate that a giant impact during terrestrial planet formation can remove several Earth oceans’ worth of H2O, and other heavier volatile species, together with a primordial hydrogen-dominated atmosphere. These results may offer an explanation for the observed depletion in Earth’s light noble gas budget and for its depleted xenon inventory, which suggest that Earth underwent significant atmospheric loss by the end of its accretion. Because planetary embryos are massive enough to accrete primordial hydrogen envelopes and because giant impacts are stochastic and occur concurrently with other early atmospheric evolutionary processes, our results suggest a wide diversity in terrestrial planet volatile budgets.
Bayesian calibration of the mixing length parameter αML and of the helium-to-metal enrichment ratio ΔY/ΔZ with open clusters: the Hyades test-bed
Authors: Tognelli E , Dell’Omodarme M , Valle G , Prada Moroni P G , Degl’Innocenti S 
Published: November 2020

ABSTRACT
We tested the capability of a Bayesian procedure to calibrate both the helium abundance and the mixing length parameter (αML), using precise photometric data for main-sequence (MS) stars in a cluster with negligible reddening and well-determined distance. The method has been applied first to a mock data set generated to mimic Hyades MS stars and then to the real Hyades cluster. We tested the impact on the results of varying the number of stars in the sample, the photometric errors, and the estimated [Fe/H]. The analysis of the synthetic data set shows that αML is recovered with a very good precision in all the analysed cases (with an error of few percent), while [Fe/H] and the helium-to-metal enrichment ratio ΔY/ΔZ are more problematic. If spectroscopic determinations of [Fe/H] are not available and thus [Fe/H] has to be recovered alongside with ΔY/ΔZ and αML, the well-known degeneracy between [Fe/H]–ΔY/ΔZ-αML could result in a large uncertainty on the recovered parameters, depending on the portion of the MS used for the analysis. On the other hand, the prior knowledge of an accurate [Fe/H] value puts a strong constraint on the models, leading to a more precise parameters recovery. Using the current set of pisa models, the most recent [Fe/H] value and the Gaia photometry and parallaxes for the Hyades cluster, we obtained the average values &lt;αML&gt; = 2.01 ± 0.05 and &lt;ΔY/ΔZ&gt; = 2.03 ± 0.33, sensitively reducing the uncertainty in these important parameters.
Genetic Architecture and Fitness of Bacterial Interspecies Hybrids
Authors: Bartke Katrin , Garoff Linnéa , Huseby Douglas L , Brandis Gerrit , Hughes Diarmaid 
Published: November 2020

Abstract
Integration of a conjugative plasmid into a bacterial chromosome can promote the transfer of chromosomal DNA to other bacteria. Intraspecies chromosomal conjugation is believed responsible for creating the global pathogens Klebsiella pneumoniae ST258 and Escherichia coli ST1193. Interspecies conjugation is also possible but little is known about the genetic architecture or fitness of such hybrids. To study this, we generated by conjugation 14 hybrids of E. coli and Salmonella enterica. These species belong to different genera, diverged from a common ancestor &gt;100 Ma, and share a conserved order of orthologous genes with ∼15% nucleotide divergence. Genomic analysis revealed that all but one hybrid had acquired a contiguous segment of donor E. coli DNA, replacing a homologous region of recipient Salmonella chromosome, and ranging in size from ∼100 to &gt;4,000 kb. Recombination joints occurred in sequences with higher-than-average nucleotide identity. Most hybrid strains suffered a large reduction in growth rate, but the magnitude of this cost did not correlate with the length of foreign DNA. Compensatory evolution to ameliorate the cost of low-fitness hybrids pointed towards disruption of complex genetic networks as a cause. Most interestingly, 4 of the 14 hybrids, in which from 45% to 90% of the Salmonella chromosome was replaced with E. coli DNA, showed no significant reduction in growth fitness. These data suggest that the barriers to creating high-fitness interspecies hybrids may be significantly lower than generally appreciated with implications for the creation of novel species.
Tirant Stealthily Invaded NaturalDrosophila melanogasterPopulations during the Last Century
Authors: Schwarz Florian , Wierzbicki Filip , Senti Kirsten-André , Kofler Robert 
Published: November 2020

AbstractIt was long thought that solely three different transposable elements (TEs)—the I-element, the P-element, and hobo—invaded natural Drosophila melanogaster populations within the last century. By sequencing the “living fossils” of Drosophila research, that is, D. melanogaster strains sampled from natural populations at different time points, we show that a fourth TE, Tirant, invaded D. melanogaster populations during the past century. Tirant likely spread in D. melanogaster populations around 1938, followed by the I-element, hobo, and, lastly, the P-element. In addition to the recent insertions of the canonical Tirant, D. melanogaster strains harbor degraded Tirant sequences in the heterochromatin which are likely due to an ancient invasion, likely predating the split of D. melanogaster and D. simulans. These degraded insertions produce distinct piRNAs that were unable to prevent the novel Tirant invasion. In contrast to the I-element, P-element, and hobo, we did not find that Tirant induces any hybrid dysgenesis symptoms. This absence of apparent phenotypic effects may explain the late discovery of the Tirant invasion. Recent Tirant insertions were found in all investigated natural populations. Populations from Tasmania carry distinct Tirant sequences, likely due to a founder effect. By investigating the TE composition of natural populations and strains sampled at different time points, insertion site polymorphisms, piRNAs, and phenotypic effects, we provide a comprehensive study of a natural TE invasion.
Benchmark dose analysis of multiple genotoxicity endpoints in gpt delta mice exposed to aristolochic acid I
Authors: Chen Ruixue , You Xinyue , Cao Yiyi , Masumura Kenichi , Ando Tomoko , Hamada Shuichi , Horibata Katsuyoshi , Wan Jingjing , Xi Jing , Zhang Xinyu , Honma Masamitsu , Luan Yang 
Published: November 2020

Abstract
As the carcinogenic risk of herbs containing aristolochic acids (AAs) is a global health issue, quantitative evaluation of toxicity is needed for the regulatory decision-making and risk assessment of AAs. In this study, we selected AA I (AAI), the most abundant and representative compound in AAs, to treat transgenic gpt delta mice at six gradient doses ranging from 0.125 to 4 mg/kg/day for 28 days. AAI-DNA adduct frequencies and gpt gene mutation frequencies (MFs) in the kidney, as well as Pig-a gene MFs and micronucleated reticulocytes (MN-RETs) frequencies in peripheral blood, were monitored. The dose–response (DR) relationship data for these in vivo genotoxicity endpoints were quantitatively evaluated using an advanced benchmark dose (BMD) approach with different critical effect sizes (CESs; i.e., BMD5, BMD10, BMD50 and BMD100). The results showed that the AAI-DNA adduct frequencies, gpt MFs and the MN-RETs presented good DR relationship to the administrated doses, and the corresponding BMDL100 (the lower 90% confidence interval of the BMD100) values were 0.017, 0.509 and 3.9 mg/kg/day, respectively. No positive responses were observed in the Pig-a MFs due to bone marrow suppression caused by AAI. Overall, we quantitatively evaluated the genotoxicity of AAI at low doses for multiple endpoints for the first time. Comparisons of BMD100 values across different endpoints provide a basis for the risk assessment and regulatory decision-making of AAs and are also valuable for understanding the genotoxicity mechanism of AAs.
Factors Associated With Pregnancy and Perinatal Outcomes in Female Neurosurgeons: A Cross-Sectional Study
Authors: Parker Samantha L , Conner Christopher R , Mata Douglas A , Zima Laura A , Chen Amber , McCormack Ryan , Bindal Shivani , Stark Jessica R 
Published: November 2020

Abstract
Neurosurgery is male dominated with women representing only 12% of residents and 5% of practicing neurosurgeons. The conflicting demands of training versus pregnancy and motherhood are significant deterrents to women entering the field. We examined pregnancy incidence and timing, perinatal complications, and the perceived career impact of motherhood on female neurosurgeons using an anonymous survey of 643 training, practicing, and retired female neurosurgeons from the United States. Among 260 respondents, 50.8% (132/260) reported pregnancies, with an average age at first pregnancy that was significantly higher than the national average (32.1 vs 26.3 yr). In all, 40.1% (53/132) of respondents reported perinatal complications in at least one of their pregnancies. Only 25% (33/132) of respondents noted designated program maternity allowances. The most significant challenges associated with being a mother and neurosurgeon reported were issues relating to work/life balance, “mommy guilt,” and sleep deprivation. A majority of respondents, 70.1% (82/116), reported fear of backlash from co-residents, partners, and staff, as well as hindered career advancement related to childbearing. Female neurosurgeons face challenges surrounding family planning different from those faced by male practitioners. Higher perinatal and fetal complications, backlash from colleagues, and demanding workload are significant issues. Progress requires institutional support and mentorship for women to create a more diverse field of practitioners.