bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects on development and microbial community of shrimp Litopenaeus vannamei larvae with probiotics treatment
Authors: Wang Ruixuan , Guo Zihan , Tang Yapeng , Kuang Jiawei , Duan Yafei , Lin Heizhao , Jiang Shigui , Shu Hu , Huang Jianhua 
Published: May 2020

Abstract
Shrimp production is the second ranked of the most-traded production in these decades and the whiteleg shrimp Litopenaeus vannamei is the sixth most cultured species. Probiotics are alternative strategy for the promotion of growth and prevention of diseases in aquaculture. To confirmed the effects of the probiotics on development and microbial community of L. vannamei larvae during different development stages, five kinds of probiotics (108~109 CFU/g ) were added into the shrimp larvae rearing environment, and the effects of probiotics on bacterial community and water quality, larval growth and immune index were determined from nauplius larval stage to post larval stage. Results suggested that probiotics treated groups showed larger survival rate than the control groups from Z1 stage to P5 stage. Lactobacillus could improve the larvae’s survival ability, especially in the larval stages M2, M3, P1, P5 stage. It was confirmed that probiotics could promote the growth and development of shrimp larvae and prevent the incomplete molting in their growing process, particularly for EM-treated group. Results suggested that all the probiotics-treated groups had shown significant decreasing trend in the quantity of vibrios, except for the SA-treated group.And different probiotics could inhibite vibrios in different life periods. Amount these probiotics, LA, EM and PB had shown the best effects, including improving survival rate of the larvae, promoting the larval metamorphosis, reducing the quantity of vibrios and NH4-N and NO2-N levels, and increasing bacterial diversity.
Effects of different stocking densities on the trachea barrier function and its plasma metabolic profile of finishing broilers
Authors: Wang Yuanyuan , Wang Dianchun , Wang Jiangshui , Li Kaixuan , Heng Chianning , Jiang Lei , Cai Chenhao , Zhan Xiuan 
Published: May 2020

Abstract
Background The experiment was conducted to evaluate the effects of different stocking densities on the trachea barrier and its plasma metabolic profile of finishing broilers.Methods One thousand four hundred and forty 22-day-old Lingnan Yellow feathered broilers were randomly allotted to five different stocking density groups (8, 10, 12, 14, and 16 birds per m2). Each group consisted of three replicates.Results A markedly increased in IL-1β and IL-10 concentrations were observed in 16 birds per m2 treatment group than the groups of 8 and 10 birds per m2 (P < 0.05), but there was no significant difference in IL-4 content among five treatments (P > 0.05). 16 birds per m2 (high stocking density group, HSD) treatment group significantly increased the trachea mucosa thickness than 10 birds per m2 (control group, CSD). HSD group exhibited lower expression of claudin1 and higher muc2 and caspase3 than those in the CSD group. In addition, a total of 10 metabolites were identified at higher (P < 0.05) concentrations, while 7 metabolites were identified at lower (P < 0.05) concentrations in the HSD treatment as compared with the CSD group. Most of these potentially diagnostic biomarkers were involved in substance synthesis and energy metabolism. Further metabolic pathway analysis revealed that pentose and glucuronate interconversions along with pentose phosphate pathway were the most relevant and critical pathway identified from these two groups. The activated pathway may partially interpret the metabolic level to alleviate the adverse effects of stress which caused by high-stocking density in broilers.Conclusions In summary, this study can improve our understanding of the harmful effects of high-stocking density on the trachea barrier and identify two metabolic pathways that may be associated with high-stocking density-induced metabolic disorders in broilers.
Uneven Integration: The Case of Angola
Authors: Haddad Eduardo Amaral , Perobelli Fernando , Araújo Inácio Fernandes , Dentinho Tomas L C 
Published: May 2020

Abstract
Angola’s prospects for reconstruction and development of its poor connectivity infrastructure are heavily dependent upon the export performance of its oil sector. Using an interregional input-output table for Angola, we estimate comprehensive measures of trade in value added revealing different hierarchies of interregional and international trade integration, with implications for regional inequality in the country. By encompassing the subnational perspective in the case study of an African country that shows a strong regional divide, we bring new insights to the existing literature on regional integration in the continent. The different hierarchies of interregional and international trade structures reveal a pattern of uneven integration of Angolan provinces, dominated by strong foreign linkages and weak domestic linkages. It also shows the primacy of Luanda in interregional trade, as this province is associated with the main trade flows involving provinces in the extended economic core of the country. The results shed light to a policy discussion about the role played by a wider and broader integration of the country including the interconnection with its neighboring countries as a way to development of the provinces in the borders, but also stimulating the potential of more central provinces, such as Huambo, Bié and Huíla.
Induction of autophagy mitigates TDP-43 pathology and translational repression of neurofilament mRNAs in mouse models of ALS/FTD
Authors: Kumar Sunny , Phaneuf Daniel , Cordeau Pierre , Boutej Hejer , Kriz Jasna , Julien Jean-Pierre 
Published: May 2020

Abstract
Background:
TDP-43 proteinopathy is a pathological hallmark of many neurodegenerative diseases including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). So far, there is no therapy available for these neurodegenerative diseases. In addition, the impact of TDP-43 proteinopathy on neuronal translational profile remains unknown.
Methods:
Biochemical, immunohistology and assay-based studies were done with cell cultures and transgenic mice models. We also used a Ribotag approach combined with microarray and proteomic analyses to investigate the neuronal translational profiles in mouse model of ALS/FTD.
Results:
Here, we report that oral administration of a novel analog (IMS-088) of withaferin-A, an antagonist of nuclear factor kappa-B (NF-ĸB) essential modulator (NEMO), induces autophagy and reduced TDP-43 proteinopathy in the brain and spinal cord of transgenic mice expressing human TDP-43 mutants, models of ALS/FTD. Treatment with IMS-088 ameliorated cognitive impairment, reduced gliosis in the brain of ALS/FTD mouse models. With the Ribotrap method, we investigated the impact of TDP-43 proteinopathy and IMS-088 treatment on the translation profile of neurons of one-year old hTDP-43A315T mice. TDP-43 proteinopathy caused translational dysregulation of specific mRNAs including translational suppression of neurofilament mRNAs resulting in 3 to 4-fold decrease in levels type IV neurofilament proteins. Oral administration of IMS-088 rescued the translational defects associated with TDP-43 proteinopathy and restored the synthesis of neurofilament proteins, which are essential for axon integrity and synaptic function.
Conclusions:
Our study revealed that induction of autophagy reduces TDP-43 pathology and ameliorates the translational defect seen in mice models of ALS/FTD. Based on these results, we suggest IMS-088 and perhaps other inducers of autophagy should be considered as potential therapeutics for neurodegenerative disorders with TDP-43 proteinopathies.
ZSCAN25 and CYP2E1 polymorphisms is risk factors for ischemic stroke in a Chinese Han population: a case control study
Authors: Yuan Haozheng , Fan Pei , Yao Li , Lv Yuying , Wei Haidong , Zheng Juan , Han Xinsheng 
Published: May 2020

Abstract
Background

We aimed to explore the relationship between ZSCAN25 and CYP2E1 polymorphisms and Ischemic stroke (IS) susceptibility among a Chinese Han population.
Methods

We enrolled 477 patients with IS and 480 age- and sex- matched health controls. Genotyping of the ZSCAN25 rs10242455, CYP2E1 rs2070672 and rs2515641 were performed by Agena MassARRAY platform. Odds ratio (OR) and 95% confidence interval (CI) were calculated by logistic regression analysis.
Results

Rs10242455 (OR = 0.56, 95% CI: 0.34–0.93, p = 0.024) was associated with a reduced IS susceptibility, while rs2070672 (OR = 1.40, 95% CI: 1.12–1.75, p = 0.003) and rs2515641 (OR = 1.29, 95% CI: 1.01–1.64, p = 0.041) with an increased IS occurrence. Rs2070672 was observed to correlate with IS risk (OR = 4.06, p = 0.038) at age > 64 years, and rs10242455 (OR = 0.45, p = 0.021) and rs2070672 (OR = 3.28, p = 0.024) affected IS risk in males. In addition, rs10242455 (OR = 1.72, p = 0.014) was significantly associated with hypertension in IS patients.
Conclusion

Our study firstly found that rs10242455 in ZSCAN25, rs2070672 and rs2515641 in CYP2E1 were associated with the occurrence of IS in a Chinese Han population.
Polysaccharide from angelica sinensis attenuates SNP-Induced Apoptosis in osteoarthritis Chondrocytes by Inducing Autophagy via ERK1/2 Pathway
Authors: Xu Chao , Ni Su , Zhuang Chao , Li Chenkai , Zhao Gongyin , Jiang Shijie , Wang Liangliang , Zhu Ruixia , Wijnen Andre J. van , Wang Yuji 
Published: May 2020

Abstract
Objective Chondrocyte apoptosis plays a vital role in osteoarthritis (OA) progression. Angelica sinensis polysaccharide (ASP), a traditional Chinese medicine, possesses anti-inflammatory and anti-apoptotic properties in chondrocytes. This study aimed to determine the protective role of ASP on sodium nitroprusside (SNP)-induced chondrocyte apoptosis, and explore the underlying mechanism. Method Human primary chondrocytes isolated from articular cartilage of OA patients were treated with SNP alone or in combination with different dose of ASP. Cell viability and apoptosis were assessed, and apoptosis-related proteins including Bcl-2 and Bax were detected. Autophagy levels were evaluated by light chain 3 (LC3)-II immunofluorescence staining, mRFP-GFP-LC3 fluorescence localisation and western blot (LC3II, p62, Beclin-1, Atg5). Meanwhile,activation of ERK 1/2 pathway was determined by western blot. The autophagy inhibitors 3-Methyladenine (3-MA), chloroquine (CQ) and a specific inhibitor of ERK1/2, SCH772984, were used respectively to confirm the autophagic effect of ASP. Results The results showed that SNP-induced chondrocyte apoptosis was significantly rescued by ASP, whereas ASP alone promoted chondrocytes proliferation. The anti-apoptotic effect of ASP was related to the enhanced autophagy and depended on the activation of ERK1/2 pathway.Conclusion ASP markedly rescued SNP-induced chondrocyte apoptosis by activating ERK1/2-dependent autophagy in chondrocytes, and it made ASP a potential therapeutic supplementation for OA treatment.
Study of a robotic system to detect water leakage and fuel debris -System proposal and feasibility study of visual odometry providing intuitive bird's eye view
Authors: Wang Zhenyu , Endo Gen , Takahashi Masashi , Nabae Hiroyuki , Suzumori Koichi , Nobuyoshi Tsuzuki , Takahashi Hideharu , Kimoto Kazushi , Ihara Tomonori , Kikura Hiroshige 
Published: May 2020

Abstract
To obtain the necessary information of the fuel debris and water leakages in the decommissioning task of Fukushima Daiichi nuclear power plants, an ultrasonic-based investigating method had been proposed towards the future internal investigation of the Primary Containment Vessel (PCV). In this article, we studied the assisting mechanisms and methods for the investigating method, as the rotatable winch mechanism and visual localization method. We used the rotatable winch mechanism to adjust the height and orientation of the ultrasonic sensor and localized the robot with cameras to localize the sensor, as assisting information for data combination. We studied the feasibility of the conventional visual odometry method of applying to the situation and performed localizing accuracy evaluating experiments with a mobile robotic platform prototype. Results showed that the visual odometry method could generate intuitive maps from bird’s eye view, and provided the average error rate of 35mm/1500mm, which met the required error rate for grating movement. The adjustable parameter ranges that could provide the required accuracy were also conducted.
Mechanical Buckling Analysis of Functionally Graded Plates Using an Accurate Shear Deformation Theory
Authors: MADJID BERRABAH HAMZA , BACHIR BOUDERBA 
Published: May 2020

Abstract
In this present study, we are interested in the use of a precise theory of shear deformation for the buckling analysis of plates with functional gradation simply supported such as the refined theory of plates with four variables, several parameters of comparison have been used, dimensional and non-dimensional, the displacement field is compatible with this study, the non-use of shear correction factors is satisfied, the choice of material is very precise in such a way are variable according to the thickness of the plate and on the other hand to make comparison with other researcher and confirms that this study gives precise results and converges, the transverse shear stresses vary through the thickness, the results found is also studied and discussed.
Development and validation of an individualized immune-related gene pairs prognostic signature in papillary renal cell carcinoma
Authors: Zhou Xianghong , Qiu Shi , Jin Di , Jin Kun , Zheng Xiaonan , Yang Lu , Wei Qiang 
Published: May 2020

Abstract
Background: Papillary renal carcinoma (PRCC) is one of the important subtypes of kidney cancer, with a high degree of heterogeneity. At present, there is still a lack of robust and accurate biomarkers for the diagnosis, prognosis and treatment selection of PRCC. Considering the important role of tumor immunity in PRCC, we aim to construct a signature based on immune-related gene pairs (IRGPs) to estimate the prognostic of patients with PRCC.Methods: We obtained gene expression profiling and clinical information of patients with PRCC from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), which were divided into discovery and validation cohorts, respectively. The immune-related genes in the samples were used to construct gene pairs, and the immune-related genes pairs (IRGPs) with robust impact for overall survival (OS) were screened out to construct the signature by univariate analysis, multivariate Cox analysis, and least absolute shrinkage and selection operator (Lasso) analysis. Then we verified the prognostic role of the signature, and assessed the relationship between this signature with tumor immune infiltration and functional pathways.Results: A total of 315 patients were included in our study, and divided to discovery (n=287) and validation (n=28) cohorts. Finally, we selected 14 IRGPs with a panel of 22 unique genes to construct the prognostic signature. According to the signature, we stratified patients into high-risk group and low-risk group. In both discovery and validation cohorts, the results of Kaplan-Meier analysis showed that there were significant differences in OS between the two groups (p<0.001). Combined with multiple clinical pathological factors, the results of multivariate analyses confirmed that this signature was an independent predictor of OS (HR, 3.548; 95%CI, 2.096−6.006; p<0.001). The results of immune infiltration analysis demonstrated that the abundance of multiple tumor-infiltration lymphocytes such as CD8+ T cells, Tregs, and T follicular cell helper were significantly higher in the high-risk group. Functional analysis showed that multiple immune-related signaling pathways were enriched in the high-risk group.Conclusions: We successfully established an individualized prognostic immune-related gene pairs signature, which can accurately and independently predict the OS of patients with PRCC.
The Effect of Crocin Supplementation on Glycemic Control, Insulin Resistance and Active AMPK Levels in Patients with Type 2 Diabetes: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
Authors: Behrouz Vahideh , Dastkhosh Ali , Hedayati Mehdi , Sedaghat Meghdad , Sharafkhah Maryam , Sohrab Golbon 
Published: May 2020

Abstract
Background Crocin as a carotenoid exerts anti-oxidant, anti-inflammatory, anti-cancer, neuroprotective and cardioprotective effects. Besides, the increasing prevalence of diabetes mellitus and its allied complications, and also patients' desire to use natural products for treating their diseases, led to the design of this study to evaluate the efficacy of crocin on glycemic control, insulin resistance and active adenosine monophosphate-activated protein kinase (AMPK) levels in patients with type-2 diabetes (T2D). Methods In this clinical trial with a parallel-group design, 50 patients with T2D received either 15-mg crocin or placebo, twice daily, for 12 weeks. Anthropometric measurements, dietary intake, physical activity, blood pressure, glucose homeostasis parameters, active form of AMPK were assessed at the beginning and at the end of the study.Results Compared with the placebo group, crocin improved fasting glucose level (P=0.015), hemoglobin A1c (P=0.045), plasma insulin level (P=0.046), insulin resistance (P=0.001), and insulin sensitivity (P=0.001). Based on the within group analysis, crocin led to significant improvement in plasma levels of glucose, insulin, hemoglobin A1c, systolic blood pressure, insulin resistance and insulin sensitivity. The active form of AMPK did not change within and between groups after intervention. Conclusions The findings indicate that crocin supplementation can improve glycemic control and insulin resistance in patients with T2D. Further studies are needed to confirm these findings. Trial Registration This study has been registered at Clinicaltrial.gov with registration number NCT04163757.
Incidence, Risk Factors and Clinical Outcomes of Acute Kidney Injury after Heart Transplantation: A Retrospective Single Center Study.
Authors: Jiang Yiyao , Kong Xiangrong 
Published: May 2020

Abstract
Objectives
This study aimed to identify the incidence rate of Acute kidney injury (AKI) in our center, assess risk factors for one-year mortality, and predict short- and long-term survival after heart transplantation (HTx).
Methods
This single-center, retrospective study from October 2009 to Jan 2020 analyzed the pre-, intra-, and postoperative characteristics of 87 patients who underwent HTx. AKI was defined according to the Kidney Disease: Improving Global Outcomes (KDIGO) criteria. Risk factors were analyzed by multivariable logistic regression models. The log-rank test was used to compare long-term survival.
Results
Twenty-six (36.1%) patients developed AKI. The one-year mortality rates in HTx patients with and without AKI were 26.92% and 10.87%, respectively (P > 0.05). Recipients who required renal replacement therapy (RRT) had a one-year mortality rate of 53.85% compared to 10.87% in those without AKI or RRT (P = 0.003). A long cardiopulmonary bypass (CPB) time (OR: 1.622, 95% CI: 1.014 to 2.595, P = 0.044) was positively related to the occurrence of AKI. A high intraoperative urine volume (OR: 0.566, 95% CI: 0.344 to 0.930, P = 0.025) was negatively correlated with AKI. AKI requiring RRT (HR, 6.402; 95% CI, 2.014–20.355, P = 0.002) was a risk factor for death. Overall survival in patients without AKI at 1, 3, and 5 years was higher than that in patients with AKI (P > 0.05).
Conclusions
AKI is common after HTx and adversely impacts early mortality. A long CPB time and low intraoperative urine volume maybe associated with the occurrence of AKI. AKI requiring RRT could contribute powerful prognostic information to predict short-term survival.
Inhibition of Jiawei Foshou San on invasion and metastasis through MMP/TIMP signaling in endometriosis
Authors: Zhang Ying , Wei Jiahui , Tan Yi , Zhang Chengling , Xu Pingli , Chen Yi 
Published: May 2020

Abstract
Background: The new formula Jiawei Foshou San (JFS) is consisted of ligustrazine, ferulic acid and tetrahydropalmatine designed from Foshou San . Previously JFS inhibited the growth of rat autograft endometriosis with unclear mechanism. To uncover the effect of JFS on invasion and metastasis in endometrial cells and xenograft endometriosis. Methods: In vitro , cell viability assay was performed for IC50 measurement in hEM15A and HEC1-B cells after treating JFS. Effects of JFS on invasion and metastasis were analyzed in scratch wound and transwell assay. In vivo , effect of JFS was evaluated in xenogeneic transplantation of endometriosis model. The gene and protein expression of MMP/TIMP signaling were inspected in vitro and in vivo . Results: Inhibitory effects of JFS were investigated with dose-dependent manner in hEM15A and HEC1-B cells. JFS significantly inhibited the invasion and metastasis in dose- and time-dependent manner. In xenograft endometriosis, JFS reduced the volume of ectopic endometrium. In-depth study, inactive MMP/TIMP signaling expressed the lower MMP-2/9, higher TIMP-1 by JFS in vitro and in vivo . Conclusions: JFS prevent invasion and metastasis via inactivation of MMP/TIMP signaling in endometrial cells and xenograft endometriosis. It reveals the potential mechanism of JFS on endometriosis and the benefit for further application.
Land Use Land Cover (LULC) Change Detection Using Geospatial Technique in Anbessa Forest, Benishangul-Gumuz Region, Ethiopia
Authors: Guyu Daie Ferede , Aduwa Tunkala Anjulo 
Published: May 2020

Abstract
Background

LULC analysis using satellite images for detecting the changes across a given landscape is a very crucial tool for understanding the nexus between forest ecosystems and human activities. LULC pattern of Anbessa forest had undergone fast changes over the last 30 years, but no research measured the level of the changes. The present study was aimed at detecting the LULC change pattern of Anbessa forest using data from satellite images between 1989 and 2019.
Methods

We examined the LULC changes of Anbessa forest using satellite image data over the period of 1989–2019 using geospatial technique.
Results

The results show a 29% and 18% decrease in land area under dense and open forests respectively over a period of 30 years. Conversely there is 32% and 25.6% increase in the land under agricultural land and settlement areas respectively. A relatively small average decrease of 8% in shrub land was found although there was a decrease from 1989 to 2004 and an increase from 2004 to 2019.
Conclusion

The fact that there is a decrease in natural and open forests and an increase in agricultural and settlement areas implies there had been fast degradation of natural forests of Anbessa forest due to human activities. Thus, there should be an intervention project that ensures the sustainability of the forest.