bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caveolin‐1 upregulates Fut8 expression by activating the Wnt/β‐catenin pathway to enhance HCC cell proliferative and invasive ability
Authors: Zhang Cheng , Wu Qiong , Huang Huang , Chen Xixi , Huang Tianmiao , Li Wenli , Zhang Jianing , Liu Yubo 
Published: July 2020

AbstractCaveolin‐1 (Cav‐1), a critical structural protein of caveolae, plays an oncogenic role by participating in abnormal protein glycosylation in hepatocellular carcinoma (HCC). However, the mechanism by which Cav‐1 regulates glycosylation and glycosyltransferase expression has yet to be fully defined. Here, we show that Cav‐1 promotes the expression of α‐1,6‐fucosyltransferase (Fut8), which catalyzes the transfer of GDP‐fucose to the core structure of the N‐sugar chain. In this study, we show that the mouse HCC cell line, Hepa1‐6, which has low Fut8 transcriptional and protein levels, also lacks Cav‐1 expression, whereas the mouse HCC cell line, Hca‐F, has strong Fut8 expression and high transcriptional and protein levels of Cav‐1. Subsequently, Cav‐1 overexpression in Hepa1‐6 was found to activate Wnt/β‐catenin signaling, which leads to downstream binding of the T cell factor/lymphoid enhancer factor to the Fut8 promoter region for activation of its transcription. In contrast, knockdown of Cav‐1 expression in Hca‐F caused the Wnt/β‐catenin pathway to be significantly inhibited, which attenuates the expression of Fut8. We further show that Cav‐1‐induced upregulation of Fut8 expression enhanced proliferation and invasion by mouse HCC cells in vitro. Our current findings provide molecular evidence that Cav‐1 plays an important role in regulating glycosyltransferase expression and may participate in abnormal glycosylation, which mediates the proliferation and invasion of HCC.
Tuning the Topological Landscape of DNA–Cyclodextrin Nanocomplexes by Molecular Design
Authors: Neva Tania , Carbajo‐Gordillo Ana I. , Benito Juan M. , Lana Hugo , Marcelo Gema , Ortiz Mellet Carmen , Tros de Ilarduya Conchita , Mendicuti Francisco , García Fernández José M. 
Published: July 2020

AbstractOriginal molecular vectors that ensure broad flexibility to tune the shape and surface properties of plasmid DNA (pDNA) condensates are reported herein. The prototypic design involves a cyclodextrin (CD) platform bearing a polycationic cluster at the primary face and a doubly linked aromatic module bridging two consecutive monosaccharide units at the secondary face that behaves as a topology‐encoding element. Subtle differences at the molecular level then translate into disparate morphologies at the nanoscale, including rods, worms, toroids, globules, ellipsoids, and spheroids. In vitro evaluation of the transfection capabilities revealed marked selectivity differences as a function of nanocomplex morphology. Remarkably high transfection efficiencies were associated with ellipsoidal or spherical shapes with a lamellar internal arrangement of pDNA chains and CD bilayers. Computational studies support that the stability of such supramolecular edifices is directly related to the tendency of the molecular vector to form noncovalent dimers upon DNA templating. Because the stability of the dimers depends on the protonation state of the polycationic clusters, the coaggregates display pH responsiveness, which facilitates endosomal escape and timely DNA release, a key step in successful transfection. The results provide a versatile strategy for the construction of fully synthetic and perfectly monodisperse nonviral gene delivery systems uniquely suited for optimization schemes.
Electrocardiographic parameters and prognosis of renal light chain amyloidosis
Authors: Li Huixian , Wang Ying , Lan Ping , Xie Liyi , Zhao Yanhong , Lu Wanhong , Li Guoliang 
Published: July 2020

AbstractBackgroundCardiac involvement frequently occurs in patients with renal light chain (AL) amyloidosis, which predisposes these patients to heart failure, arrhythmia, or infarction with poor prognosis.HypothesisTwelve‐lead electrocardiogram (ECG) parameters may be associated with prognosis in renal AL amyloidosis.MethodsA retrospective single‐center cohort study was performed. Biopsy‐proven renal AL amyloidosis patients from January 2014 to December 2018 at the First Affiliated Hospital of Xi'an Jiaotong University were enrolled. The baseline demographic information, laboratory tests, 12‐lead ECG parameters at the time of diagnosis were obtained from medical records. The endpoint was defined as the time to all‐cause death from baseline for all deceased patients and time to censor date (June 2019) for all other patients. Univariate and multivariate Cox proportional hazard models were conducted to identify the relationship between ECG parameters and all‐cause mortality.ResultsA total of 69 patients with a mean age of 61.5 ± 11.4 years were enrolled in this study. The median PR interval and QTc interval were 160 (140, 186) and 417 ± 42 ms. The mean follow‐up duration was 15.9 ± 13.8 months. Multivariate Cox regression analysis showed that regardless of adjustment for age, gender and serum creatinine, PR interval (HR 1.022, 95% CI: 1.007‐1.038, P = .005), and QTc interval (HR 1.012, 95% CI: 1.004‐1.021, P = .004) were independently associated with all‐cause mortality.ConclusionsPR interval and QTc interval were independently associated with all‐cause mortality in renal AL amyloidosis patients. ECG parameters may provide prognostic potential of renal AL amyloidosis patients and promote the management of patients with renal AL amyloidosis.
Carborane‐Containing Matrix Metalloprotease (MMP) Ligands as Candidates for Boron Neutron‐Capture Therapy (BNCT)
Authors: Lutz Marlon R. , Flieger Sebastian , Colorina Andre , Wozny John , Hosmane Narayan S. , Becker Daniel P. 
Published: July 2020

AbstractBased on the previously reported potent and selective sulfone hydroxamate inhibitors SC‐76276, SC‐78080 (SD‐2590), and SC‐77964, potent MMP inhibitors have been designed and synthesized to append a boron‐rich carborane cluster by employing click chemistry to target tumor cells that are known to upregulate gelatinases. Docking against MMP‐2 suggests binding involving the hydroxamate zinc‐binding group, key H‐bonds by the sulfone moiety with the peptide backbone residues Leu82 and Leu83, and a hydrophobic interaction with the deep P1’ pocket. The more potent of the two triazole regioisomers exhibits an IC50 of 3.7 nM versus MMP‐2 and IC50 of 46 nM versus MMP‐9.
Synthesis and Pharmacological Evaluation of Fluorinated Quinoxaline‐Based κ‐Opioid Receptor (KOR) Agonists Designed for PET Studies
Authors: Tangherlini Giovanni , Börgel Frederik , Schepmann Dirk , Slocum Samuel , Che Tao , Wagner Stefan , Schwegmann Katrin , Hermann Sven , Mykicki Nadine , Loser Karin , Wünsch Bernhard 
Published: July 2020

Abstractκ‐Opioid receptors (KORs) play a predominant role in pain alleviation, itching skin diseases, depression and neurodegenerative disorders such as multiple sclerosis. Therefore, imaging of KOR by a fluorinated PET tracer was envisaged. Two strategies were followed to introduce a F atom into the very potent class of cis,trans‐configured perhydroquinoxalines. Whereas the synthesis of fluoroethyltriazole 2 has already been reported, fluoropyrrolidines 14 (1‐[2‐(3,4‐dichlorophenyl)acetyl]‐8‐[(R)‐3‐fluoropyrrolidin‐1‐yl]‐perhydroquinoxalines) were prepared by SN2 substitution of a cyclic sulfuric acid derivative with hydroxypyrrolidine and subsequent transformation of the OH moiety into a F substituent. Fluoropyrrolidines 14 showed similar low‐nanomolar KOR affinity and selectivity to the corresponding pyrrolidines, but the corresponding alcohols were slightly less active. In the cAMP and β‐arrestin assay, 14b (proton at the 4‐position) exhibited similar KOR agonistic activity as U‐50,488. The fluoro derivatives 14b and 14c (CO2CH3 at the 4‐position) revealed KOR‐mediated anti‐inflammatory activity as CD11c and the IFN‐γ production were reduced significantly in mouse and human dendritic cells. Compounds 14b and 14‐c also displayed anti‐inflammatory and immunomodulatory activity in mouse and human T cells. The PET tracer [18F]‐2 was prepared by 1,3‐dipolar cycloaddition. In vivo, [18F]‐2 did not label KOR due to very fast elimination kinetics. Nucleophilic substitution of a mesylate precursor provided [18F]‐14c. Unfortunately, defluorination of [18F]‐14c occurred in vivo, which was analyzed in detail by in vitro studies.
Independent link between peripheral artery disease and muscle wasting in patients with heart failure
Authors: Ohori Katsuhiko , Yano Toshiyuki , Katano Satoshi , Kouzu Hidemichi , Inoue Takuya , Takamura Yuhei , Nagaoka Ryohei , Ishigo Tomoyuki , Koyama Masayuki , Nagano Nobutaka , Fujito Takefumi , Nishikawa Ryo , Miura Tetsuji 
Published: July 2020

AbstractAimsA high prevalence of muscle wasting, that is, reduction in muscle mass, in patients with peripheral artery disease (PAD) and heart failure (HF) has been reported. However, whether the association between PAD and muscle wasting is independent of shared risk factors such as diabetes mellitus has not been examined.Methods and resultsWe retrospectively enrolled 440 HF patients (mean age, 74 years; inter‐quartile range, 64–82 years; 52% male). Muscle wasting was defined as an appendicular skeletal muscle mass index (ASMI) of <7.0 kg/m2 in men and <5.4 kg/m2 in women. PAD was defined as an ankle brachial index (ABI) of <0.9 in either leg. The prevalence of PAD in HF patients was 21%. ASMI was positively correlated with ABI in HF patients. In multivariate logistic regression analysis, ASMI and muscle wasting were selected as independent explanatory factors of the presence of PAD after adjustment for age, sex, diabetes mellitus, hypertension, dyslipidaemia, estimated glomerular filtration rate, and smoking status, established risk factors of atherosclerosis. In propensity score‐matched analysis, frequency of muscle wasting was higher in patients with PAD than in patients with an ABI of ≧1.1 (72.1% vs. 52.5%, P = 0.04).ConclusionsThe results suggest that there is an independent link between PAD and muscle wasting in HF patients.
Amperometric Tyrosinase Biosensor Based on Carbon Black Paste Electrode for Sensitive Detection of Catechol in Environmental Samples
Authors: Wong Ademar , Santos Anderson M. , Fatibello‐Filho Orlando , Sotomayor Maria D. P. T. 
Published: July 2020

AbstractIn this work, a renewable tyrosinase‐based biosensor was developed for the detection of catechol, using a carbon black paste electrode, without any mediator. The effect of pH, type of electrolyte, and amount of tyrosinase enzyme were explored for optimum analytical performance. The best‐performing biosensor in amperometric experiments at potential −0.2 V vs. Ag/AgCl (3 mol L−1 KCl) was obtained using a 0.1 mol L−1 phosphate buffer solution (pH 7.0) as electrolyte. Under optimized conditions, the proposed biosensor had two concentration linear ranges from 5.0×10−9 to 4.8×10−8 and from 4.8×10−8 to 8.5×10−6 mol L−1 and a limit of detection of 1.5×10−9 mol L−1. The apparent Michaelis‐Menten constant (
) was calculated by the amperometric method, and the obtained value was 1.2×10−5 mol L−1 whose result was similar when compared with other studies previously. The biosensor was applied in river water samples, and the results were very satisfactory, with recoveries near 100 %.In addition, the response of this biosensor for different compounds, taking into account their molecular structures was investigated and the results obtained showed no interference with the response potential of catechol. The electrochemical biosensor developed in this work can be considered highly advantageous because it does not require the use of a mediator (direct detection) for electrochemical response, and also because it is based on a low‐cost materials that can be used with success to immobilise other enzymes and/or biomolecules.
Voltammetric Detection of Nitrite Anions Employing Imidazole Functionalized Reduced Graphene Oxide as an Electrocatalyst
Authors: Tajiki Alireza , Abdouss Majid , Sadjadi Sodeh , Mazinani Saeedeh 
Published: July 2020

AbstractA kind of metal free catalyst with excellent electrocatalytic activity toward nitrite anions oxidation was synthesized through functionalization of graphene oxide (GO) with imidazole aromatic rings. Imidazole rings linked to graphene oxide nanosheets via 3‐chloropropyl trimethoxysilane (CPTMS) mediators following simultaneous thermally reduction of GO during the functionalization, to form rGO−Im for modification of gold electrode (GE). The sensing platform characterized by SEM, XRD, IR and Raman methods. The resulting rGO−Im‐GE sensor, responded sensitively to nitrite concentrations within a wide linear range (1 to 1000 μM) and indicated a relative low detection limit (LOD=0.28 μM, S/N=3) without interfering with common ions existing in aqueous media. Furthermore acceptable recoveries were obtained when applying the proposed sensor to tap water samples.
Inhibition of Mild Steel Corrosion in Acidic Medium by Extract of Spilanthes Uliginosa Leaves
Authors: Durodola Solomon S. , Adekunle Abolanle S. , Olasunkanmi Lukman O. , Oyekunle John A. O. 
Published: July 2020

AbstractCorrosion of mild steel in 2.0 M HCl was investigated in the presence of various concentration of methanolic extract of Spilanthes uliginosa leaves. Inhibition efficiency was evaluated by gravimetric and electrochemical methods and was found to increase with increase in concentration of the extract. The results showed that inhibitive actions of the extract occurred as a result of adsorption of molecular constituents of the leave extract on mild steel in acidic medium. Potentiodynamic polarization results confirmed that the extract act as mixed‐type inhibitor.
Voltammetric Study of the Electrocatalytic Oxidation of Formaldehyde on Pt−Pd Co‐catalyst Supported on Reduced Graphene Oxide¶
Authors: Galal Ahmed , Hassan Hagar K. , Atta Nada F. 
Published: July 2020

AbstractElectrocatalysis on chemically converted graphene and decorated with Pt−Pd mixed‐catalyst (CCG/Pt−Pd) is described. Graphene sheets were chemically synthesizeed via microwave‐assisted method and modified with Pt−Pd mixed‐catalyst that was formed electrochemically. The hybrid layer, CCG/Pt−Pd displayed current oscillations for the electro‐oxidation of formaldehyde. Several factors influence both the number of oscillation in current peaks and their frequencies such as formaldehyde concentration, catalyst loading, potential scan rate and window, and temperature. CCG/Pt−Pd has high electrocatalytic activity toward formaldehyde conversion and increased oscillations were obtained at lower concentrations. Apparent activation energy for the oxidation process wasEa, of 6.29 kJ.mol−1.
Draining solar drying of sewage sludge: Experimental study and modeling
Authors: Masmoudi Azza , Ben Sik Ali Ahlem , Dhaouadi Hatem , Mhiri Hatem 
Published: July 2020

AbstractThe draining solar drying is considered as an innovative solution in the field of sanitation. The objective of this work is to investigate the feasibility and model the kinetics of the sludge solar drying in a lab‐scale draining greenhouse by varying the air flow rate during winter and summer. The first experiment was conducted without ventilation. Then, a series of experiences was carried out with increasing the air flow. The drying period varied between 3 and 6 days in summer and between 9 and 14 days in winter. By increasing the air velocity, the drying time decreased. For the drying rate curves, in summer, only the falling rate period existed. However, in winter, the curves did not follow any regular shape of drying kinetics. Nine mathematical equations existing in the literature were tested to identify the thin‐layer drying kinetics of sewage sludge inside the draining greenhouse. The Midilli‐Kucuk model was the most reliable for describing the evolution of reduced moisture content versus drying time in hot and cold periods. However, for different drying conditions, the Wang and Singh model was the least suitable. The results revealed also that the effective diffusivity increased when the air flow rate and the ambient temperature increased.
Optimization of energy, economic, and environmental indices in sunflower cultivation: A comparative analysis
Authors: Aghili Nategh Nahid , Banaeian Narges , Gholamshahi Alireza , Nosrati Mohammad 
Published: July 2020

AbstractThis study examined the input energy, economic indices, and Greenhouse Gas (GHG) emissions in sunflower farm enterprises of Kermanshah province of Iran. Different mechanization production systems involving traditional, semi‐mechanized, and mechanized ones were statistically compared. Results revealed that mechanized farms consumed more total inputs energy, while possessed significantly higher yield and better economic indices. In which, the human labor, diesel fuel, and fertilizer were the most predominant inputs in GHG emissions. In particular, traditional, semi‐mechanized and mechanized farms emitted 358, 386, and 438 kg CO2/ha, respectively. Also, technical efficiencies were reported as 0.88, 0.86, and 0.96, for traditional, semi‐mechanized, and mechanized farms, respectively. The relationship among different variables including energy inputs, GHG emissions, output energy, and benefit to cost ratio was studied using econometric modeling. Data envelopment analysis (DEA) and multi‐objective genetic algorithm (MOGA) were also applied to detect a set of Pareto frontiers in the combination of energy, environmental, and economic indices (energy consumption, GHG emissions, and benefit to cost ratio as three selected output parameters) for sunflower production. It has been observed that the capability of MOGA for energy saving was higher than DEA. Application results of DEA and MOGA combined algorithms showed that diesel fuel and water had the highest and lowest potential for total energy savings, respectively.
Preparation and surface characteristics of phosphoric acid‐activated carbon from coconut shell in air
Authors: Higai Daisuke , Huang Zhebin , Qian Eika W. 
Published: July 2020

AbstractA process of chemical activation with phosphoric acid from coconut shell in air to produce activated carbon was developed. The effects of various preparation parameters on the porous texture and surface characteristics were investigated by means of the nitrogen physisorption, Fourier transform‐infrared spectra (FT‐IR), and X‐ray photoelectron spectroscopy (XPS). The adsorption capacities of activated carbon for chromium, lithium, and cesium were also investigated. The surface modification of activated carbon using nitric acid was performed to enhance the adsorption capacities, and the relationship between the adsorption capacities and the surface functional groups was investigated. The oxygen‐containing functional groups on the surface of an activated carbon played a more important role than its textural structure in adsorption of metal ions, especially cesium ion.
Phytoremediation potential of coffee pod (Cassia tora): An in situ approach for attenuation of chromium from overburden soil of Sukinda Chromite Mine, India
Authors: Patra Deepak Kumar , Grahacharya Aradhana , Pradhan Chinmay , Patra Hemanta Kumar 
Published: July 2020

AbstractPot experiments were conducted for 60 days to investigate the phytoremediation potential of Cassia tora grown in different concentrations of chromium (Cr) rich overburden soil (OBS) of Sukinda Chromite Mine (10, 20, 30, 40, 50, 70, and 100%). To assess the phytoaccumulation ability of C. tora, the tolerance index (TI), bio‐concentration factor (BCF), transportation index (Ti), and total accumulation rate (TAR) were measured. The results showed that at 100% OBS the TI, BCF, Ti, and TAR of C. tora have 23.89, 0.28, 60.25, and 7.46, respectively. The Cr recovery from contaminated soil by C. tora was notable as reported to be 6.25, 11.6, 12.75, 14.2, 17.35, 22.65, and 17.7 mg/kg, respectively. The outcome indicated that C. tora is a potential phytoremediator of Cr from contaminated sites due to its high bioaccumulation activity, high tolerance, and Ti. Therefore, C. tora can be suggested for phytostabilization program to lessen toxic Cr from chromite overburden mining sites.
Pretreatment methods affecting the color, flavor, bioactive compounds, and antioxidant activity of jujube wine
Authors: Cai Wenchao , Tang Fengxian , Shan Chunhui , Hou Qiangchuan , Zhang Zhendong , Dong Yun , Guo Zhuang 
Published: July 2020

AbstractIn the case of wine production, the selection of optimal pretreatment methods and starter cultures are the 2 key points before fermentation. In this research, the fresh jujube was separately underwent alcoholic fermentation at 20°C with 3 different pretreatment methods (with peel, without peel, and juice) and 5 different starter cultures, respectively. Color analysis, electronic sense analysis, bioactive compound analysis, and antioxidant activity analysis combined with multivariate statistical analysis were applied to evaluated the effects of pretreatment methods and starter cultures on the overall quality of jujube wine. It was found that both pretreatment methods and starter cultures have effects on the quality of jujube wines, in which pretreatment methods have much more significant effects. The jujube wines fermented with different pretreatment methods were classified clearly by their overall quality, and that of the jujube wines fermented with peel was the best among all, since it can not only enhance the color and flavor quality of the wine, but also maximize the preservation of bioactive compounds and antioxidant activity of jujube for better consumer acceptance. This will provide a theoretical reference and application basis for the quality improvement of jujube wine.
Do servant, transformational, transactional, and passive avoidant leadership styles influence mentoring competencies for faculty? A study of a gender equity leadership development program
Authors: Sims Cynthia , Carter Angela , Moore De Peralta Arelis 
Published: July 2020

AbstractThis study examined whether women and men who were more skilled in one leadership style—servant, transformational, transactional, or passive avoidant—were better mentors and assessed if gender influenced leadership style or mentoring. Faculty (n = 56) who were members of one of two cohorts, participated in leadership development programs focused on gender equity at a university in the southeast U.S. The study used a quantitative cross‐sectional survey design and the units of analysis were individual program participants. Initial regression analysis revealed servant leadership was positively and statistically associated with mentoring and passive avoidant leadership was negatively and statistically associated with mentoring. Transformational and transactional leadership were not statistically associated with mentor competency. Gender was not found to be associated with leadership style or mentoring. Human Resource Development professionals and those who conduct gender equity and other leadership development programs should consider the benefits of servant leadership due to its gender‐neutral style and synergistic ability to develop leaders as skilled mentors.