bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disrupted Blood-Retina Lysophosphatidylcholine Transport Impairs Photoreceptor Health But Not Visual Signal Transduction
Authors: Lobanova Ekaterina S. , Schuhmann Kai , Finkelstein Stella , Lewis Tylor R. , Cady Martha A. , Hao Ying , Keuthan Casey , Ash John D. , Burns Marie E. , Shevchenko Andrej , Arshavsky Vadim Y. 
Published: November 2019

Retinal photoreceptor cells contain the highest concentration of docosahexaenoic acid (DHA) in our bodies, and it has been long assumed that this is critical for supporting normal vision. Indeed, early studies using DHA dietary restriction documented reduced light sensitivity by DHA-deprived retinas. Recently, it has been demonstrated that a major route of DHA entry in the retina is the delivery across the blood–retina barrier by the sodium-dependent lipid transporter, Mfsd2a. This discovery opened a unique opportunity to analyze photoreceptor health and function in DHA-deprived retinas using theMfsd2aknock-out mouse as animal model. Our lipidome analyses ofMfsd2a−/−retinas and outer segment membranes corroborated the previously reported decrease in the fraction of DHA-containing phospholipids and a compensatory increase in phospholipids containing arachidonic acid. We also revealed an increase in the retinal content of monounsaturated fatty acids and a reduction in very long chain fatty acids. These changes could be explained by a combination of reduced DHA supply to the retina and a concomitant upregulation of several fatty acid desaturases controlled by sterol regulatory element-binding transcription factors, which are upregulated inMfsd2a−/−retinas.Mfsd2a−/−retinas undergo slow progressive degeneration, with ∼30% of photoreceptor cells lost by the age of 6 months. Despite this pathology, the ultrastructureMfsd2a−/−photoreceptors and their ability to produce light responses were essentially normal. These data demonstrate that, whereas maintaining the lysophosphatidylcholine route of DHA supply to the retina is essential for long-term photoreceptor survival, it is not important for supporting normal phototransduction.SIGNIFICANCE STATEMENTPhospholipids containing docosahexaenoic acid (DHA) are greatly enriched in the nervous system, with the highest concentration found in the light-sensitive membranes of photoreceptor cells. In this study, we analyzed the consequences of impaired DHA transport across the blood–retina barrier. We have found that, in addition to a predictable reduction in the DHA level, the affected retinas undergo a complex, transcriptionally-driven rebuilding of their membrane lipidome in a pattern preserving the overall saturation/desaturation balance of retinal phospholipids. Remarkably, these changes do not affect the ability of photoreceptors to produce responses to light but are detrimental for the long-term survival of these cells.
Iron Metabolism in the Peripheral Nervous System: The Role of DMT1, Ferritin, and Transferrin Receptor in Schwann Cell Maturation and Myelination
Authors: Santiago González Diara A. , Cheli Veronica T. , Wan Rensheng , Paez Pablo M. 
Published: November 2019

Iron is an essential cofactor for many cellular enzymes involved in myelin synthesis, and iron homeostasis unbalance is a central component of peripheral neuropathies. However, iron absorption and management in the PNS are poorly understood. To study iron metabolism in Schwann cells (SCs), we have created 3 inducible conditional KO mice in which three essential proteins implicated in iron uptake and storage, the divalent metal transporter 1 (DMT1), the ferritin heavy chain (Fth), and the transferrin receptor 1 (Tfr1), were postnatally ablated specifically in SCs. Deleting DMT1, Fth, or Tfr1in vitrosignificantly reduce SC proliferation, maturation, and the myelination of DRG axons. This was accompanied by an important reduction in iron incorporation and storage. When these proteins were KOin vivoduring the first postnatal week, the sciatic nerve of all 3 conditional KO animals displayed a significant reduction in the synthesis of myelin proteins and in the percentage of myelinated axons. Knocking out Fth produced the most severe phenotype, followed by DMT1 and, last, Tfr1. Importantly, DMT1 as well as Fth KO mice showed substantial motor coordination deficits. In contrast, deleting these proteins in mature myelinating SCs results in milder phenotypes characterized by small reductions in the percentage of myelinated axons and minor changes in theg-ratio of myelinated axons. These results indicate that DMT1, Fth, and Tfr1 are critical proteins for early postnatal iron uptake and storage in SCs and, as a consequence, for the normal myelination of the PNS.SIGNIFICANCE STATEMENTTo determine the function of the divalent metal transporter 1, the transferrin receptor 1, and the ferritin heavy chain in Schwann cell (SC) maturation and myelination, we created 3 conditional KO mice in which these proteins were postnatally deleted in Sox10-positive SCs. We have established that these proteins are necessary for normal SC iron incorporation and storage, and, as a consequence, for an effective myelination of the PNS. Since iron is indispensable for SC maturation, understanding iron metabolism in SCs is an essential prerequisite for developing therapies for demyelinating diseases in the PNS.
Caregiver Medication Management and Understanding After Pediatric Hospital Discharge
Authors: Philips Kaitlyn , Zhou Roy , Lee Diana S. , Marrese Christine , Nazif Joanne , Browne Constance , Sinnett Mark , Tuckman Steven , Griffith Kimberly , Kiely Victoria , Lutz Marcia , Modi Anjali , Rinke Michael L. 
Published: November 2019

OBJECTIVES:Caregivers frequently make mistakes when following instructions on discharge medications, and these instructions often contain discrepancies. Minimal literature reflects inpatient discharges. Our objective was to describe failures in caregiver management and understanding of inpatient discharge medications and to test the association of documentation discrepancies and sociodemographic factors with medication-related failures after an inpatient hospitalization.METHODS:This study took place in an urban tertiary care children’s hospital that serves a low-income, minority population. English-speaking caregivers of children discharged on an oral prescription medication were surveyed about discharge medication knowledge 48 to 96 hours after discharge. The primary outcome was the proportion of caregivers who failed questions on a 10-item questionnaire (analyzed as individual question responses and as a composite outcome of any discharge medication–related failure). Bivariate tests were used to compare documentation errors, complex dosing, and sociodemographic factors to having any discharge medication-related failure.RESULTS:Of 157 caregivers surveyed, 70% had a discharge medication–related failure, most commonly because of lack of knowledge about side effects (52%), wrong duration (17%), and wrong start time (16%). Additionally, 80% of discharge instructions provided to caregivers lacked integral medication information, such as duration or when the next dose after discharge was due. Twenty five percent of prescriptions contained numerically complex doses. In bivariate testing, only race and/or ethnicity was significantly associated with having any failure (P = .03).CONCLUSIONS:The majority of caregivers had a medication-related failure after discharge, and most discharge instructions lacked key medication information. Future work to optimize the discharge process to support caregiver management and understanding of medications is needed.
Metastatic Thymoma Harboring a Deleterious BRCA2 Mutation Derives Durable Clinical Benefit from Olaparib
Authors: Principe Daniel R. , Kamath Suneel D. , Munshi Hidayatullah G. , Mohindra Nisha A. 
Published: November 2019

Abstract

Thymomas comprise a group of rare epithelial neoplasms of the anterior mediastinum. Whereas localized disease carries a favorable prognosis, the majority of patients with metastatic thymomas experience progression or recurrence over a 10-year period. Although targeted therapies have become standard of care in many malignancies, no clinically actionable mutations have consistently been identified in metastatic thymomas. Here, we describe a patient with an aggressive thymoma complicated by extensive pleural metastases. Over a 16-year period, she progressed on multiple treatment regimens. To identify additional treatment options, tissue from a pleural metastasis was sent for next-generation sequencing, revealing mutations in BRCA2, tyrosine kinase 2, and SET domain containing 2. Based on supporting evidence for poly (ADP-ribose) polymerase (PARP) inhibition in other BRCA-mutated tumors, the patient was started on the PARP inhibitor olaparib. She derived significant clinical benefit from treatment, with imaging showing overall stabilization of her disease. Here, we review the genotyping results of her tumor and discuss the functional and clinical significance of the mutations in her cancer as well as implications for managing patients with advanced BRCA-mutant thymomas.

Key Points
Targeted therapy has yet to enter the standard clinical management of metastatic thymomas. Patients with BRCA2-mutant thymomas may benefit from poly (ADP-ribose) polymerase inhibition.
Editorial
Authors: Aguirre-Ramírez Néstor Jaime , Gómez Botero Maryory Astrid , Orozco-Arroyave Juan Rafael , Osorio Juan Pablo , Villegas-Ramírez Juan Guillermo 
Published: November 2019

In 2018, the School of Engineering of Universidad de Antioquia (UdeA) celebrated its 75th anniversary. Within this commemoration, the School organized ExpoIngenieria 2018, a broad forum for the interchange of knowledge between Academia, Industry, and the State. Having “Development and sustainability” as a leitmotif, this event included several parallel activities, namely: an international congress with 3307+ attendants, the business and innovation roundtable Tecnnova 2018 with 2100+ business meetings and the fifth Workshop on Engineering Applications of Computer Science with 130+ attendants and 91 contributed papers, among others.Remarkably, the main Engineering fair (ExpoIngenieria 2018) reached a broad public of more than 28000 attendants, ranging from high school students to researchers and industrial practitioners. The Expo was the opportunity to join together more than 42 research groups from academia and industry. The goal of the Expo was to showcase the contributions generated in the Engineering field by the UdeA School of Engineering as well as other partner universities in a broad range of traditional, new and emerging domains such as: Energy and environment; Materials, chemistry and bioengineering; Infrastructure, mobility and logistics; and, Telecommunication, information technologies and computer science.As part of this celebration, we edited this special issue of Redin - Revista Facultad de Ingeniería. The call was open and received seven submissions from academics and practitioners. After a rigorous review process, we proudly present four papers that were accepted and are published in this issue of Redin. These four papers include one in the renewable energy field (Edwin Chica, Fredys Romero Menco, Ainhoa Rubio Celemnte. “Design of a wave energy converter system for the Colombian Pacific Ocean) and three in alternative (natural) materials, its processing and applications (R. Prakash, R. Thenmozhi, Sudharshan N. Raman, C. Subramanian. “Mechanical behavior of polypropylene fibre reinforced concrete containing waste coconut shell as coarse aggregates and fly ash as partial cement replacement; Darío Alfonso Páez Soto, Luis Jorge Herrera Fernández, Oscar Esneider Acosta Agudelo, Marcelo Herrera Martínez. “Design and construction of an acoustic conditioning panel made from typical Colombian fibres”; Estefanía Echeverri Correa, David Orlando Grajales Lopera, Santiago Gutiérrez Restrepo, Claudia Patricia Ossa Orozco. “Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution”).
Surface Morphology of Spermatheca Structure In Some Terellia Rob-Des (Diptera: Tephritidae) Species: A Scanning Electron Microscope Study
Authors: KÜTÜK Murat , DOĞAN Esra 
Published: November 2019

Spermatheca structures of 6
species [Terellia fuscicornis (Loew,
1844), T. longicauda (Meigen, 1838), T. luteola (Wiedemann, 1830), T. nigripalpis Hendel, 1927, T. serratulae (Linneaus, 1758) and T. virens (Loew, 1846)] belonging to
genus Terellia Robineau - Desvoidy
(Diptera: Tephritidae) were photographed with scanning electron microscopy
(SEM). Obtained figures were examined
and ultrastructure of the spermatheca for each species were described and
differences between species were discussed. Also SEM micrographs of each
species were presented.
Alfabetismo jurídico en las facultades de derecho en Colombia: ¿Fundamental en la democracia y el orden social?
Authors: CARREÑO DUEÑAS DALIA , MORENO DURÁN ÁLVARO HERNÁN , PACHECO BAQUERO JANETH MILENA 
Published: November 2019

El presente artículo de reflexión se deriva de la investigación “Competencias ciudadanas y participación comunitaria” realizada en Fontibón localidad de Bogotá por investigadores de la facultad de derecho la universidad Santo Tomás.
El artículo tiene como objetivo hacer una reflexión y una propuesta que contribuya a las debilidades de la democracia y el estado de derecho de algunas las comunidades. La disertación parte de la idea central del escrito que plantea que el desorden del Estado es debido en parte a la desobediencia de las normas jurídicas de los ciudadanos que posiblemente tenga en una de sus fuentes la ignorancia de las mismas normas y el desconocimiento de principios tanto políticos como de sus derechos y deberes ciudadanos, que por la misma situación no han interiorizado estas reglas del juego lo que se traduce en carencia de conductas positivas al respecto.
Frente a la problemática se expone la estrategia de un alfabetismo jurídico apoyado por la investigación acción participativa entre las facultades de derecho y las comunidades en Colombia dentro del marco constitucional vigente y el espíritu humanístico de la academia.
A arte, a obra, o mundo
Authors: Oliveira Luiz Sérgio da Cruz 
Published: November 2019

Para muitos, a arte nunca esteve nem tampouco está afastada de sua dimensão crítica. Quer tenha colorações políticas e/ou ideológicas ou se apresente simplesmente como apolítica, a arte se mantém diligente diante das coisas do mundo, o que seria suficiente para assegurar e mesmo consagrar a criticidade da arte, independentemente da vontade, do desejo ou mesmo da intencionalidade do artista criador. No entanto, essas afirmações, lançados ao vento na expectativa de sua replicação acrítica ad infinitum, parecem comprometidas com a naturalização de mitos que tendem a transformar o processo de criação artística em narrativas fabulosas, empurrando a arte para os confins de um imaginário no qual a arte parece dissociada do mundo, ao contrário do que supõe sua dimensão crítica.
Resiliens
Authors: Strambi Marta Luiza 
Published: November 2019

O presente artigo contempla a convergência teoria e prática, envolvendo o desenvolvimento poético de obras que foram produzidas sob o fulgor de um pensamento artístico que tem como condição sua dimensão crítica, as relações com a autobiografia e seus campos ‘borrados’ nas modalidades em questão. Para tanto ele inclui a instalação Campo de forças sob o conceito de artificialidade e resistência à vida e, sua discussão sobre o campo em “perigo”, ações que sobrepõem “o que se quer” ao “que não podemos dominar”.
PENINGKATAN KEMAMPUAN PEMECAHAN MASALAH MATEMATIS MELALUI MODEL PEMBELAJARAN LAPS-HEURISTIK
Authors: Azwardi Gilang , Sugiarni Rani 
Published: November 2019

Abstrak: Penelitian ini bertujuan untuk menjawab apakah setelah penerapan model LAPS-Heuristic(Logan Avenue Problem Solving) dapat meningkatkan kemampuan pemecahan masalah matematis siswa pada materi trigonometri di kelas X SMA Negeri 1 Cibeber. Jenis penelitian kuantitatif tipe Quasi Experimen dengan bentuk Design The Nonequivalent Prettest-Posttest Control Group Design. Populasi dalam penelitian ini adalah siswa kelas X IPA SMAN 1 Cibeber. Adapun penelitian ini dipilih sebanyak 2 kelas dari 3 kelas melalui teknik purposive samplingdan melibatkan sampel sebanyak 53 orang yang berasal dari dua kelas yang pilih. Kelas X IPA 2 dipilih sebagai kelas eksperimen yang memperoleh pembelajaran dengan model LAPS-Heuristik dan kelas X IPA 1 dipilih sebagai kelas kontrol yang memperoleh pembelajaran biasa. Untuk mendapatkan data hasil penelitian digunakan instrument berupa tes kemampuan pemecahan masalah matematis siswa dan kuisioner sikap siswa. Pengolahan data hasil penelitian ini menggunakan software SPSS versi 20. Berdasarkan hasil analisis data penelitian kemampuan pemecahan masalah matematis siswa dengan menggunkan model pembelajaran LAPS-Heuristic lebih baik di bandingan menggunakan model pembelajaran biasa. Sikap siswa terhadap pembelajaran matematika dengan menggunakan model LAPS-Heuristic(Logan Avenue Problem Solving)sebagian besar positif.
Katakunci: Model LAPS-Heuristic; Kemampuan Pemecahan Masalah Matematis.
Mean Free Path of Gas Molecules in Organic Nanochannels Using Molecular Simulations
Authors: Baek Seunghwan , Yucel Akkutlu I.. 
Published: November 2019

SummaryA computational method using molecular–simulation data is introduced to estimate the average mean–free–path length of multicomponent hydrocarbon molecules in an organic nanochannel. Grand–canonical Monte Carlo (MC) simulation is used first to construct the equilibrium distribution of the gas molecules in the channel. These results show that the smaller the channel is, the denser the gas mixture becomes because of nanoconfinement effects. Capillary condensation occurs in the smaller channels. The fluid composition inside a channel becomes progressively heavier when the bulk–fluid pressure outside the nanopore is reduced and the lighter hydrocarbons leave the channel. The mean–free–path lengths of the confined molecules are computed using the trajectories of the molecules displaced over time in the equilibrium molecular–dynamics (MD) simulation. The average length of the confined molecules is estimated to be an order of magnitude smaller than the theoretical value. Further, the length does not show a strong dependence on the channel width and the pressure. Consequently, the predicted Knudsen–number value does not vary significantly, as anticipated by the kinetic theory of gases and by the molecular simulations of pure fluids. This invariance indicates that compositional change caused by nanoconfinement eliminates transition into other transport regimes where continuum mechanics is no longer valid.
Compound heterozygous LPIN2 pathogenic variants in a patient with Majeed syndrome with recurrent fever and severe neutropenia: case report
Authors: liu jun , Hu Xu-Yun , Zhao Zhi-Peng , Guo Ruo-Lan , Guo Jun , Li Wei , Hao Chan-Juan , Xu Bao-Ping 
Published: November 2019

Abstract
Background: Majeed syndrome is a rare, autosomal recessive autoinflammatory disorder first described in 1989. The syndrome starts during infancy with recurrent relapses of osteomyelitis typically associated with fever, congenital dyserythropoietic anemia (CDA), and often neutrophilic dermatosis. Mutations in the LPIN2 gene located on the short arm of chromosome 18 have been identified as being responsible for Majeed syndrome.
Case presentation: We report an 8-month-old boy, who presented with recurrent fever, mild to moderate anemia, and severe neutropenia. Erythrocyte sedimentation rate and C-reactive protein were elevated. Molecular testing identified a paternal splicing donor site variant c.2327+1G>C and a maternal frameshift variant c.1691_1694delGAGA (Arg564Lysfs*3) in LPIN2.
Conclusions: Only a few cases with LPIN2 mutation have been reported, mainly in the Middle East with homozygous variants. Our patient exhibited a mild clinical phenotype and severe neutropenia, different from previous reports.
Autotransplantation of mature impacted tooth to a fresh molar socket using a 3D replica and guided bone regeneration: two years retrospective case series
Authors: Wu Ye , Chen Jiaming , Xie Fuping , Liu Huanhuan , Niu Gang , Zhou Lin 
Published: November 2019

Abstract
Background: The aim of this study was to evaluate the clinical outcome of autotransplantation of mature third molars to fresh molar extraction sockets using 3D replicas.
Methods: Ten patients underwent teeth autotransplantation with or without GBR. We observed the mobility, percussion, radiography examination, the probing depth and the masticatory function of the transplanted teeth during two years following up, which were transplanted into fresh molar sockets by using 3D replicas, and GBR when it is necessary.
Results: The average extra-oral time of donor tooth had been shortened to 1.65 min when used the 3D replica. Some probing depth of the transplanted tooth were deeper than 3 mm at 4 or 5 weeks temporarily. And one patient felt slight sensitive when chewing with soft food at 4 weeks, then disappeared. The clinical examination of the autotransplantation teeth during one year follow-up showed no sign of failure.
Conclusions: The tooth autotransplantation using 3D replica with or without GBR is an effective method which can reduce the extra-oral time of the donor teeth and may result in less failure.
Point of care testing evaluation of lateral flow immunoassay for diagnosis of Cryptococcus Meningitis in Kenya, 2017
Authors: Gitonga Lawrence Kirimi , Boru Waqo Gufu , Kwena Arthur , Maritim Marybeth , Wamicwe Joyce , Ransom James Anthony 
Published: November 2019

Abstract
Objectives: The objective of this study was to evaluate the performance of lateral flow immunoassay (LFA) against latex agglutination (LA), India ink and culture in point-of-care diagnosis of Cryptococcus meningitis (CM). We conducted a cross-sectional study among patients with suspected CM at Mbagathi Hospital, Nairobi, April-July 2017.
Results: Of 124 capillary blood and serum and 99 cerebrospinal fluid (CSF) samples, the agreement between LFA and LA on serum was 94.4%, kappa (0.88), sensitivity (100%) and specificity (91%). LFA and LA on CSF, was 97.9%, kappa (0.96), sensitivity (100%) and specificity (96%). LFA and India ink was 96.9%, kappa (0.94), sensitivity (100%) and specificity (94.1%). On CSF culture, the agreement was 72.7%, kappa (0.43), sensitivity (100%) and specificity (64%). The agreement of LFA on capillary blood, serum and CSF was 100% with kappa (1.00), sensitivity and specificity of 100%
Multidisciplinary training program for the promotion of physical activity and other healthy habits in adolescents: justification, design and methodology of PAP study
Authors: Sánchez-Miguel Pedro Antonio , Vaquero-Solís Mikel , Sánchez-Oliva David , González Juan José Pulido , Serrano Miguel Angel Tapia 
Published: November 2019

Abstract
Overweight and obesity are one of the most important health problems worldwide. Physical inactivity is indicated as one of the most determining factors of overweight and obesity. The Self-Determination Theory points out the importance of motivation in the patterns related to physical activity. The aims of this study were to show the reasons for performing and the methods of a randomised controlled trial o evaluate the effectiveness of a multidisciplinary physical activity programme to promote physical activity levels in inactive school children and encourage healthy lifestyles, carried out under the Self-Determination Theory postulates. The present paper presents a RCT for adolescents of 1st and 2nd grades of Secondary Compulsory School. The multidisciplinary training program consists of two parts: the first part is cross-sectional, and the second part, the Physical Activity Program, is of a longitudinal nature. This project takes into account the socio-economic impact that a healthy lifestyle will generate, as well as its potential to apply the results and transfer them to the productive sector. This study is of great interest to the scientific community as it will provide relevant information on physical inactivity issues, sedentary lifestyle, generation of healthy habits and physical exercise.