bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single Cell Transcriptional Profiling of Phox2b-Expressing Geniculate Ganglion Neurons
Authors: Anderson Catherine B. , Larson Eric D. 
Published: October 2019

AbstractThe sense of taste is fundamental for survival as harmful substances can be discriminated and prevented from entering the body. Taste buds act as chemosensory sentinels and detect bitter, salty, sweet, sour, and umami substances and transmit signals to afferent nerve fibers. Whether a single gustatory nerve fiber selectively is responsive to a single taste modality (through taste receptor cell activation) is a point of contention in the field.. In the present study, we present a method for single cell RNA sequencing of gustatory geniculate ganglion neurons and compare the results obtained to two prior published works. Additionally, independent reanalysis of the raw data from these previous studies confirms molecular heterogeneity of ganglion neurons. Multiple gustatory clusters are found, and we compare cluster markers identified by the original works and those identified in the present study. Across all datasets and analyses, specific clusters show a high degree of correlation including a somatosensory cluster (Phox2b-, Piezo2+, Fxyd2+), a potential sweet-best cluster (Phox2b+, Spon1+, Olfm3+), and a potential sour-best cluster (Phox2b+, Penk+, Htr3a+). Additionally, a putative mechanosensitive gustatory cluster with an unknown functional role is identified (Phox2b+, Piezo2+, Calb1+). Other gustatory clusters (Phox2b+) are more varied across analyses, but are marked by Olfm3. Which, if any, clusters comprise umami-best, bitter-best, or salty-best fibers will require further study.
Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle
Authors: He Wanwan , Xu Wenbo , Xu Letian , Fu Kaiyun , Guo Wenchao , Bock Ralph , Zhang Jiang 
Published: October 2019

AbstractTransplastomic potato plants expressing double-stranded RNA (dsRNA) targeted against essential genes of the Colorado potato beetle (CPB) can be lethal to larvae by triggering an RNA interference (RNAi) response. High accumulation levels of dsRNAs in plastids are crucial to confer an efficient RNAi response in the insects. However, whether length and sequence of the dsRNA determine the efficacy of RNAi and/or influence the level of dsRNA accumulation in plastids is not known. Here we compared the RNAi efficacy of different lengths of dsRNA targeted against the CPB β–Actin gene (ACT) by feeding in vitro-synthesized dsRNAs to larvae. We show that, while the 60 bp dsRNA induced only a relatively low RNAi response in CPB, dsRNAs of 200 bp and longer caused high mortality and similar larval growth retardation. When the dsRNAs were expressed from the plastid (chloroplast) genome of potato plants, we found that their accumulation levels were correlated with length. dsRNA accumulation levels were positively associated with the observed mortality, suppression of larval growth and suppression of target gene expression. Importantly, transplastomic potato plants expressing the 200 bp dsRNA were better protected from CPB than plants expressing the 297 bp dsRNA, the best-performing line in our previous study. Our results suggest that the length of dsRNAs is an important factor that influences their accumulation levels in plastids and thus determines the strength of the insecticidal RNAi effect. Our findings will aid the design of optimized dsRNA expression constructs for plant protection by plastid-mediated RNAi.
Efficacy of chronic ultrasound neurostimulation on behaviors and distributed brain metabolism in depressive-like mice
Authors: Legrand Marc , Galineau Laurent , Novell Anthony , Planchez Barbara , Brizard Bruno , Leman Samuel , Tauber Clovis , Escoffre Jean-Michel , Lefèvre Antoine , Gosset Philippe , El-Hage Wissam , Emond Patrick , Belzung Catherine , Bouakaz Ayache 
Published: October 2019

AbstractMajor depression is one of the main factors contributing to the Global Burden of Disease. Current treatment strategies (e.g., antidepressants and neurostimulation techniques) of major depression show some limitations including inaccuracy and invasiveness. Ultrasound neurostimulation (USNS) has been recently introduced as a physical non-invasive method for brain tissue stimulation and has gained increasing interest. In this study, we sought to evaluate the efficacy of transcranial USNS in an unpredictable chronic mild stress (UCMS) mouse model. The results show that transcranial USNS of the infralimbic cortex reduced anxiety-related behaviors as well as some, but not all, depression-related parameters. [18F]-FDG microPET imaging and brain metabolomic analyses showed that USNS triggered the activation of targeted brain region in addition to brain areas at a distance from the targeted zone, alleviating anxiety and depression-related behaviors induced by the UCMS regimen. Transcranial ultrasound neurostimulation show therapeutic potential in some aspects of major depression.
GPI-anchored SKU5/SKS are maternally required for integument development in Arabidopsis
Authors: Zhou Ke 
Published: October 2019

ABSTRACTGlycosylphosphatidylinositol-anchored proteins (GPI-APs) play crucial roles in various processes in eukaryotes. In Arabidopsis, SKS1, SKS2, SKS3 and SKU5 from SKU5/SKS gene family could encode GPI-anchored proteins, and they were recently reported to regulate cell polar expansion and cell wall synthesis redundantly in roots. Here, we report that, they are also redundantly crucial for seed production and seed morphogenesis in Arabidopsis through regulating maternal integument development. Their loss-of-functions resulted in disrupted development of integuments that failed to protect embryo sacs from exposure to external space due to physical restriction, and lead to female gametophytic abortion. Interestingly, those less defective ovules could be fertilized and develop into seeds normally, however, their seed morphogenesis was largely affected.Our research made SKS1, SKS2, SKS3 an SKU5 be not only the first class of GPI-anchored proteins that could regulate maternal integument development, but also the first class of proteins that could regulate cell polar expansion in both root and integument cells besides several MAPK cascade components. Our study also underlined the regulation by integument development in reproductive processes.
In pursuit of self-reliance – perspectives of refugees in Jordan
Authors: Shalan Muna 
Published: October 2019

Purpose
The purpose of this paper is to explore the employment experiences of refugees, who have benefited from job opportunities in Jordan. It investigates the impact of employment on their livelihoods and the related challenges that they face.

Design/methodology/approach
The research presented in this paper is of exploratory nature. By ways of analyzing selected interviews with refugees, it explores their employment experiences in terms of their motivation to work and thus provides insights into how refugees perceive the impact of employment on their progress toward self-reliance. It also incorporates the perspectives of employers and investigates measures taken by the employers to overcome challenges and facilitate refugees’ employment. The study is not meant to draw conclusions about the general trends of refugee employment or to measure refugee self-reliance. The study sample thus relies on a small number of people since it seeks depth rather than breadth in data collection.

Findings
The interpretation of the collected accounts explores how the indicative domains of self-reliance arise in the accounts of the interviewees. Findings suggest that regardless of gender, the employment process was transformational in terms of young refugees’ progress toward self-reliance, whereas its impact was less significant on older refugee women with child-rearing responsibilities. Although older refugee women with children acknowledge the positive impact of employment on their livelihoods, the accounts that they tell suggest that humanitarian assistance still plays an important role in their livelihoods. Furthermore, the collected accounts highlighted aspects that the interviewed women find problematic. These include age-restrictions that some employers impose when hiring, in addition to the limited employment opportunities for men living in Zaatari camp.

Research limitations/implications
The research limitation is primarily methodological and is attributed to the limited respondent sample size. This implies that the sample is not representative; therefore, results may lack generalizability.

Practical implications
This study highlighted multi-faceted and complex factors influencing refugee self-reliance. Therefore, it includes implications for the development of refugee employment programs to become more effective in enhancing refugee livelihoods and self-reliance. For example, it highlights that central actors engaged in employment programs for refugees should duly consider the local context to produce context-based solutions. Furthermore, it also emphasizes that central actors should not lose sight of the people-based factors such as refugees’ age, gender and child-rearing responsibilities when devising the employment programs.

Originality/value
This paper offers insights into how employment can become more effective in promoting the progressive development of refugee self-reliance in Jordan.
Re-imaging Rosetta historic core through Space Syntax
Authors: Hegazi Yasmine Sabry , Fouda Mohanad 
Published: October 2019

Purpose
The purpose of this paper is to develop strategies of re-imaging the Rosetta historic district through choosing the suitable uses and their specific locations in compliance with the urban design fabric of the historic core. These strategies are to be fulfilled using Space Syntax as an urban analysis tool, in the context of “connectivity analysis.”

Design/methodology/approach
The research methodology follows the combined strategy between three methods of research: a SWOT analysis, to reveal the historic core status; an experimental research approach, which stimulates the chosen area via Space Syntax; and the third is a case study of the Rosetta historic core.

Findings
The re-usage of the Rosetta old core was not originally planned as commercial and to host movable vendors, but the core was forced to adapt afterwards to suit this usage, while the Souk and those big new residential building urban blocks have clearly deformed the historic image of the Rosetta historic core. Moving from one space to another, it was found that well-connected spaces have higher movement density, such as Al Souk Street, while less dense spaces can tolerate more movement without conflicting with highly dense ones. The existing commercial activities can be classified into movable activities – which can be easily relocated – and shop-based, which need a developmental approach in their original locations. The disconnected district can be used as a commercial zone for the movable vendors, to which human flow can be successfully directed in order to reduce the density in the more connected spaces.

Originality/value
The research value lies in exploring how to re-image urban heritage via relocating the places reused with unsuitable activities through Space Syntax.
Experiments in interfaces
Authors: Mancke Carol Jane 
Published: October 2019

Purpose
Exploring the need for “neutral” public space located between the private act of voting and formal deliberative democracy, the purpose of this paper is to examine two interfaces between everyday life and democratic politics and considers ways this territory can be a site for generative artistic practises.

Design/methodology/approach
Many artists and architects work in the space between the individual and formal collective political processes. Speculating outward from two artworks by the author and drawing on the thought of Hannah Arendt, Rosalyn Deutsche, Chantal Mouffe, Bruno Latour and others, this paper maps theory to the territory and proposes a new framework for reconsidering the work of such practitioners.

Findings
Three potentially fruitful avenues for exploration as artistic practice related to democratic interfaces are identified and discussed through examples.

Originality/value
This exploration is part of a broader practice-led research project into models of public collaborative thinking within the context of artistic practice. Many argue that the public realm has been co-opted by neo-liberal political and economic forces, resulting in a sense of hopelessness that limits the ability to imagine anything else. This research reflects on artistic tactics that counter this sense of hopelessness. These practices often suggest alternative social structures, foster ephemeral (local) public spheres or propose spatial configurations that support these. This paper offers a useful framework for reflecting on the work of politically engaged artists and architects as well as structuring new projects.
Characterisation of fresh fruit consumption in Spain based on food-related lifestyle
Authors: Montero-Vicente Luis , Roig-Merino Bernat , Buitrago-Vera Juan , Sigalat-Signes Enrique 
Published: October 2019

Purpose
The purpose of this paper is to describe fresh fruit consumers in Spain according to their food-related lifestyle (FRL).

Design/methodology/approach
A random stratified sample of 500 people, representative of the persons responsible for household food purchasing and resident in Spain, was interviewed in 2017 using a revised and adapted version of the FRL instrument (Grunert et al., 1993). Questions about fruit purchasing criteria, consumption habits and demographics were also included. Factor and cluster analysis (Ward method) yielded four segments.

Findings
The segments identified are: “Total indifference (TI)”, small segment with disinterest in extra-domestic and social consumption, nutrition and innovation; “Little time to cook, concerned about nutrition and extra-domestic consumption (LICNE)”, the largest consumers of fresh fruit who show interest in nutrition and health, but no interest in the price of products, convenience foods or liking cooking; “Cooks and preference for natural products (COOKNAT)”, the largest segment, with a medium-high consumption of fresh fruits, who are related to cooking at home, natural products and a concern for the price-quality ratio; and “Unconcerned (UNC)” presents the lowest fresh fruit consumption and shows the lowest interest in natural products and some indifference to the other criteria.

Originality/value
The information obtained in this study gives interesting new insights for the marketing strategies of the fresh fruit suppliers to Spain and the Food and Public Health Administrations.
Does the denomination taste better than the wine?
Authors: Vecchio Riccardo , Rinaldi Alessandra , Moio Luigi 
Published: October 2019

Purpose
The purpose of this paper is twofold: first, to investigate whether consumer preferences differ for wines with diverse price points and second, to analyze the effect of denominations of origin information on preferences.

Design/methodology/approach
In, total 150 regular wine buyers expressed their willingness-to-pay (WTP) for 12 red wines of 3 denominations (Chianti Classico DOCG, Morellino di Scansano DOCG and Toscana IGT) with three price points (basic=€5, medium=€10 and high=€20) in an incentive-compatible experiment, involving two consecutive rounds: a blind tasting and an informed tasting.

Findings
The findings reveal that price points are not statistically related to consumer preferences measured in the blind round, while information on the denomination of origin leads to a statistically significant increase in WTP for wines of all price points for all denominations.

Originality/value
This paper investigates whether Sangiovese-based wines at varying levels of price points (basic, medium and high) receive different WTP by consumers in non-hypothetical experimental auctions. Furthermore, the effect of denominations of origin information on preferences is explored, comparing evaluations without and with this specific information.
An optimized hybrid model based on artificial intelligence for grape price forecasting
Authors: Chu Xiaoquan , Li Yue , Tian Dong , Feng Jianying , Mu Weisong 
Published: October 2019

PurposeThe purpose of this paper is to propose an optimized hybrid model based on artificial intelligence methods, use the method of time series forecasting, to deal with the price prediction issue of China’s table grape.Design/methodology/approachThe approaches follows the framework of “decomposition and ensemble,” using ensemble empirical mode decomposition (EEMD) to optimize the conventional price forecasting methods, and, integrating the multiple linear regression and support vector machine to build a hybrid model which could be applied in solving price series predicting problems.FindingsThe proposed EEMD-ADD optimized hybrid model is validated to be considered satisfactory in a case of China’ grape price forecasting in terms of its statistical measures and prediction performance.Practical implicationsThis study would resolve the difficulties in grape price forecasting and provides an adaptive strategy for other agricultural economic predicting problems as well.Originality/valueThe paper fills the vacancy of concerning researches, proposes an optimized hybrid model integrating both classical econometric and artificial intelligence models to forecast price using time series method.
Biotechnology in food
Authors: Charlebois Sylvain , Somogyi Simon , Music Janet , Cunningham Caitlin 
Published: October 2019

Purpose
The purpose of this paper is to measure Canadian attitudes towards genetic engineering in food, for both plant-based and livestock, assess trust towards food safety and overall regulatory system in Canada.

Design/methodology/approach
This exploratory study is derived from an inductive, quantitative analysis of primary data obtained from an online survey of adults, aged 18 and over, living in Canada for at least 12 months. An online survey was widely distributed in both French and English. Data were collected from 1,049 respondents. The sample was randomized using regional and demographic benchmarks for an accurate representation of the Canadian population. The completion rate of the survey was 94 per cent. Based on the sampling design, the margin of error is 3.1 per cent, 19 times out of 20.

Findings
Consumers misunderstand the nature of genetic engineering or do not appreciate its prevalence in agrifood or both. In total, 44 per cent of Canadians are confused about health effects of genetically engineered foods and ingredients. In total, 40 per cent believe that there is not significant testing on genetically engineered food to protect consumers. In total, 52 per cent are uncertain on their consumption of genetically engineered food, despite its prominence in the agrifood marketplace. Scientific literacy of respondents on genetic engineering is low. While Canadians are divided on purchasing genetically engineered animal-based products, 55 per cent indicated price is the most important factor when purchasing food.

Research limitations/implications
More research is required to better appreciate the sociological and economic dimensions of incorporating GM foods into our lives. Most importantly, longitudinal risks ought to be better understood for both plant- and animal-based GM foods and ingredients. Additional research is needed to quantify the benefits and risks of GM crops livestock, so business practices and policies approach market expectations. Significantly, improving consumers’ scientific literacy on GM foods will reduce confusion and allow for more informed purchasing decisions. Indeed, a proactive research agenda on biotechnologies can accommodate well-informed discussions with public agencies, food businesses and consumers.

Originality/value
This exploratory study is one of the first to compare consumers’ perceptions of genetic engineering related to animal and plant-based species in Canada since the addition of genetically modified salmon to the marketplace.
Navigating through institutional complexity: adoption of a process view in functional organizations
Authors: Henk Anastasiya , Fallmyr Terje 
Published: October 2019

PurposeThe purpose of this paper is to contribute to the ongoing debate on the appropriate organizational design for the process management implementation. Using the lens of institutional theory, the paper discusses how organizations adapt to a required implementation of a process view alongside their organizational structures.Design/methodology/approachThe study is designed as a single case study of a Norwegian shipping company. On the one hand, shipping companies are traditionally managed by functions due to the specifics of maritime operations and high safety-related risks of the work. On the other hand, the rising demands of regulatory bodies and customers within the offshore logistics are calling for implementation of a process view within the organizations, which implies management by processes.FindingsThe study analyses conflicting requirements of the institutional environment influencing organizational structure and how these conflicts are addressed by the company. Besides, it describes the decoupling mechanism the company uses to balance between such requirements and adapt to the changes of the institutional pressures.Originality/valueThe study introduces a situational-based organizational structure as an alternative for both process and vertical views implementation within the companies operating in the highly demanding institutional environments.
Learning clusters, MOOCs, free videos and organization learning: a case study from Indian SMEs
Authors: Roy Shirshendu , Bhattacharya Samar , Das Prasun 
Published: October 2019

Purpose
This paper shows how organizations can use learning clusters, Massive Open Online Courses (MOOCs) and free videos to improve organizational learning. The paper presents implementation steps through a case study with Indian micro, and small and medium-sized enterprises (SMEs).

Design/methodology/approach
As a part of the employee development strategy, most Indian organizations adapted structured training framework. However, employee skill and competency development are equally important for SMEs. An attempt was made to train employees of these sectors using MOOCs and free videos. Twenty Indian organizations were selected for the pilot study and segregated into two clusters for efficient implementation of the concept. The learning process was observed for the next six months to summarize the outcome.

Findings
The case study concluded that MOOCs and free videos made a difference in skill and competency development of SMEs using cost-effective internet and mobile handset. It also revealed the positive impact of this learning framework on productivity, the quality which eventually improved the revenue.

Research limitations/implications
This study explores the usability of MOOCs and free videos for learning purpose. More studies on learning effectiveness are required to generalize results.

Practical Implementation
This study reveals the effectiveness of MOOCs and free videos for employee development. The foundation result will help the Learning and Development (L&D) professionals and senior management to think in a different way while making the employee development strategy.

Originality/value
This research increases the knowledge base related to the use of MOOCs and free videos for employee training.