bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barriers and enablers to implementing mental well‐being programs through Australian rural football clubs—A qualitative descriptive study
Authors: Hutchesson Heidi , Dollman James , Baker Amy , Kernot Jocelyn 
Published: May 2020

AbstractIssue addressedSuicide rates in rural Australia are almost twice as high as those in urban areas. In rural communities, football clubs are often the ‘hub’ of the community and are being explored as an avenue to deliver mental health and well‐being promotion. The aim of this study was to explore barriers and enablers for programs promoting mental health and well‐being through rural Australian football clubs.MethodThis qualitative descriptive study included 12 individuals of 10 rural clubs affiliated with the South Australian National Football League. Recruitment occurred via emails to club secretaries/presidents. Semi‐structured telephone interviews explored mental health and well‐being issues experienced in clubs, previous involvement with mental well‐being programs and potential barriers/enablers for future programs. Interviews were transcribed verbatim and analysed thematically.ResultsThematic analysis identified three themes encompassing barriers and enablers: (1) more than a football club, (2) attitudes towards mental health and (3) what is needed to implement a program. The third theme had subthemes of resources, importance of timing, mental health initiatives and components of a program.ConclusionKey barriers included difficulty getting people involved due to individual attitudes towards mental health, and not having the finances/resources to implement a program. Major enablers included the important role the football club serves in the community, the inclusion of speakers with credibility and making the program engaging.So what?This study identifies key factors which may impact on community engagement and program effectiveness for mental health and well‐being programs delivered via rural football clubs.
Poor academic performance in offspring of survivors with childhood or adolescent central nervous system tumor in Sweden
Authors: Huang Wuqing , Sundquist Kristina , Sundquist Jan , Ji Jianguang 
Published: May 2020

AbstractThe number of children who were born after their parents were diagnosed with central nervous system (CNS) tumor is increasing, but it remains largely unknown regarding the academic performance of these children. We aimed to investigate whether children of survivors with childhood or adolescent CNS tumor were associated with poor academic performance. Children of survivors of CNS tumor were identified by combining the nationwide Swedish Cancer Register and the Multi‐Generation Register, and those who have completed compulsory education in Sweden between 1989 and 2015 were included in our study. “Poor academic performance” was defined as a z‐score of the academic performance below the 10th percentile. Conditional logistic regression and quantile regression were used to examine the association. A total of 655 children were born after their parental diagnosis of CNS tumor and they had 1.39 times higher risk of achieving poor academic performance as compared to the matched comparisons (95% CI = 1.10‐1.76). The poor academic performance was even more pronounced in boys, among those with a paternal diagnosis of CNS tumor and those with a parental ependymoma. The observed association differed depending on preterm birth. In addition, the strength of the association declined with the increased quantiles of academic performance z‐score. Our data suggest that parental CNS tumor affects the subsequent academic achievements among children born after the parental tumor.
Interleukin‐22 and intestinal homeostasis: Protective or destructive?
Authors: Shohan Mojtaba , Dehghani Razieh , Khodadadi Ali , Dehnavi Sajad , Ahmadi Reza , Joudaki Nazanin , Houshmandfar Sheyda , Shamshiri Marziye , Shojapourian Samira , Bagheri Nader 
Published: May 2020

AbstractInterleukin (IL)‐22 is a member of IL‐10 family cytokines with various immunologic functions. As its name implies, IL‐22 is known to be secreted mainly by Th22 cells, a recently discovered lineage of CD4+ T cells. Also, Th17, Th1, natural killer cells, γδT cells, and innate immune cells along with some nonlymphoid cells have been confirmed as secondary cellular sources of IL‐22. Different cell types such as bronchial and intestinal epithelial cells, keratinocytes, hepatocytes, dermal fibroblasts, and tubular epithelial cells are affected by IL‐22. Both pathologic and protective roles have been attributed to IL‐22 in maintaining gut homeostasis and inflammation. According to the latest fast‐growing investigations, IL‐22 is significantly involved in various pathologies including allergic diseases, infection, autoimmunity, and cancer development. Regulating gut immune responses, barrier integrity, and inflammation is dependent on a diverse complex of cytokines and mediators which are secreted by mucosal immune cells. Several investigations have been designed to recognize the role of IL‐22 in gastrointestinal immunity. This article tries to discuss the latest knowledge on this issue and clarify the potential of IL‐22 to be used in the future therapeutic approaches of intestinal disorders including inflammatory bowel diseases and colon cancer.
The halogenation of natural flavonoids, baicalein and chrysin, enhances their affinity to human protein kinase CK2
Authors: Marzec Ewa , Świtalska Marta , Winiewska‐Szajewska Maria , Wójcik Jacek , Wietrzyk Joanna , Maciejewska Agnieszka M. , Poznański Jarosław , Mieczkowski Adam 
Published: May 2020

AbstractA series of halogenated derivatives of natural flavonoids: baicalein and chrysin were designed and investigated as possible ligands for the catalytic subunit of tumor‐associated human kinase CK2. Thermal shift assay method, in silico modeling, and high‐performance liquid chromatography‐derived hydrophobicity together with IC50 values determined in biochemical assay were used to explain the ligand affinity to the catalytic subunit of human protein kinase CK2. Obtained results revealed that substitution of baicalein and chrysin with halogen atom increases their binding affinity to hCK2α, and for 8‐chlorochrysin the observed effect is even stronger than for the reference CK2 inhibitor—4,5,6,7‐tetrabromo‐1H‐benzotriazole. The cytotoxic activities of the baicalein and chrysin derivatives in the in vitro model have been evaluated for MV4‐11 (human biphenotypic B myelomonocytic leukemia), A549 (human lung adenocarcinoma), LoVo (human colon cancer), and MCF‐7 (human breast cancer) as well as on the nontumorigenic human breast epithelial MCF‐10A cell lines. Among the baicalein derivatives, the strongest cytotoxic effect was observed for 8‐bromobaicalein, which exhibited the highest activity against breast cancer cell line MCF‐7 (IC50 10 ± 3 μM). In the chrysin series, the strongest cytotoxic effect was observed for unsubstituted chrysin, which exhibited the highest activity against leukemic cell line MV4‐11 (IC50 10 ± 4 μM).
Three‐dimensional printing of extracellular matrix (ECM)‐mimicking scaffolds: A critical review of the currentECMmaterials
Authors: Da Silva Kate , Kumar Pradeep , Choonara Yahya E. , du Toit Lisa C. , Pillay Viness 
Published: May 2020

AbstractThe loss of tissues and organs through injury and disease has stimulated the development of therapeutics that have the potential to regenerate and replace the affected tissue. Such therapeutics have the benefit of reducing the reliance and demand for life‐saving organ transplants. Of the several regenerative strategies, 3D printing has emerged as the forerunner in regenerative attempts due to the fact that biologically and anatomically correct 3D structures can be fabricated according to the specified need. Despite the progress in this field, improvement is still limited by the difficulty in fabricating scaffolds that adequately mimic the native cellular microenvironment. In response, despite the complexities of the native extracellular matrix (ECM), the inclusion of ECM components into bioinks has emerged as a cutting‐edge research area in terms of providing possible ECM‐mimicking abilities of the 3D printed constructs. Furthermore, the development of ECM‐mimicking scaffolds can potentially assist in improving personalized patient treatments. This review provides a critical analysis of selected naturally occurring ECM components as well as synthetic self‐assembling peptides in their ability to provide the required ECM microenvironment for tissue regeneration. The success and possible short comings of each material, as well as the specific characteristics of each bioink, are evaluated.
Exosomal lncRNAs NONMMUT000375.2 and NONMMUT071578.2 derived from titanium particle treated RAW264.7 cells regulate osteogenic differentiation of MC3T3‐E1 cells
Authors: Xu Jie , Li Deng , Cai Zhiqing , Sun Hao , Su Baohua , Qiu Meiling , Ma Ruofan 
Published: May 2020

AbstractPeriprosthetic osteolysis and the subsequent aseptic loosening can lead to the failure of joint replacement. Wear particles are well known to be the initiative cause inducing osteolysis through enhancing osteoclast‐mediated bone resorption and reducing osteogenic differentiation. The purpose of this study was to investigate the effects of osteoclast‐secreted exosomal long noncoding RNAs (lncRNAs) on osteogenesis in the process of particle‐induced osteolysis. RAW264.7 cells were treated by titanium particles (TI). The inflammatory cytokines were increased, and expression of Receptor Activator of Nuclear Factor‐κB and Nuclear factor of activated T cells c1 were also increased, indicating osteoclast differentiation occurred. The purified exosomes from RAW264.7 cells induced with TI inhibited osteogenic differentiation of MC3T3‐E1 cells. RNA sequencing generated lncRNAs expression profiles (458 up‐regulated and 1641 down‐regulated) of the exosomes derived from RAW264.7 cells treated with TI. Based on the results of gene ontology/Kyoto Encyclopedia of Genes and Genomes analysis and quantitative real time polymerase chain reaction validation, we confirmed two candidate lncRNAs, NONMMUT000375.2 and NONMMUT071578.2. The regulation network presented that some vital genes involved in osteoclast differentiation, such as Bcl2, Wnt11, TGF‐β, and Pdk1, were under the regulation of NONMMUT000375.2 and NONMMUT071578.2. Taken together, exosomes derived from TI treated RAW264.7 cells inhibit the osteogenic activity of MC3T3‐E1 cells. Exosomal lncRNAs, NONMMUT000375.2 and NONMMUT071578.2 may potentially play their roles in promoting osteoclast differentiation and suppressing osteogenesis, which aggravates the osteoclastogenesis/osteogenesis imbalance.
Ag2Cr2O7 nanoparticles: An eco‐friendly and reusable catalyst for synthesis of pyrano[c]chromenediones and [1,3]dioxolo[g]chromeneones in aqueous media at ambient temperature
Authors: Ebrahimi Mitra , Abdolmohammadi Shahrzad , Kia‐Kojoori Reza 
Published: May 2020

AbstractAn efficient and green protocol for the synthesis of 4‐aryl‐3,4‐dihydro‐2H,5H‐pyrano[3,2‐c]chromene‐2,5‐diones and 8‐aryl‐7,8‐dihydro‐6H‐[1,3]dioxolo[4,5‐g]chromene‐6‐ones through the Ag2Cr2O7 nanoparticles catalyzed cyclocondensation reaction of active methylene compounds including 4‐hydroxycoumarin or 3,4‐methylenedioxyphenol, aromatic aldehydes, and meldrum's acid in water at ambient temperature was described. This method demonstrates several advantages compared with methods that are currently employed such as a mild reaction conditions, simple work‐up, good to excellent yields, avoiding toxic catalyst and hazardous solvent, and recovery and reuse of the catalyst.
Exploration of financial stress indicators in a developing economy
Authors: Utkarsh  , Pandey Asheesh , Ashta Arvind , Spiegelman Eli , Sutan Angela 
Published: May 2020

AbstractFinancial stress scales need to be incorporated into the borrower selection procedure before providing a loan. The three financial stress scales that we examined have strong correlations, but the two involving at least eight questions are better for discriminating between poor and rich. The financial stress indicator that separates male and female students the most is whether financial problems interfere with relationships with other people (interference is more for men). Older students are less satisfied with their financial position and seem to think that their parents worry more about disappointing them for materialistic reasons.
Determining the attributes of gastronomic tourism experience: Applying impact‐range performance and asymmetry analyses
Authors: Pratt Stephen , Suntikul Wantanee , Agyeiwaah Elizabeth 
Published: May 2020

AbstractCooking classes are a good example of third‐generation gastronomic tourism experiences. It is important to determine which cooking class experience attributes are important to participants and assess the performance of these experiences. We assess the importance and performance of cooking class experiences and their impact on overall satisfaction. We first apply the conventional importance‐performance analysis approach, critically assessing the shortcomings of this approach. Next, we use three‐factor customer satisfaction theory with the alternative approaches of impact range‐performance analysis and impact‐asymmetry analysis to determine these impacts. The findings have marketing and operational implications by identifying which cooking class experience attributes to highlight and deliver.
DJ‐1 plays a neuroprotective role in SH‐SY5Y cells by modulating Nrf2 signaling in response to lidocaine‐mediated oxidative stress and apoptosis
Authors: Xue Ying , Wang Ai‐Zhong 
Published: May 2020

AbstractTo investigate the effects of DJ‐1 on lidocaine‐induced cytotoxicity in neurons and the link with Nrf2 signaling, SH‐SY5Y cells were treated with 1, 4, 8, and 16 mM lidocaine. Cell viability was measured by MTT assay, and apoptosis was measured by flow cytometry analysis. The mitochondrial membrane potential, reactive oxygen species (ROS) levels, lipid peroxidation (MDA), and GSH/GSSG ratio were determined with specific kits. Expression of DJ‐1, Nrf2, and Nrf2 downstream signaling proteins (glutathione peroxidase [GPx], heme oxygenase‐1 [HO‐1], catalase [CAT], and glutathione reductase [GR]), was determined by western blot and qRT‐PCR. The cell viability was dramatically decreased, while levels of apoptosis, ROS and Cys106‐oxidized DJ‐1 were significantly enhanced following treatment with lidocaine (concentration 4‐16 mM), and increases were observed in a dose‐dependent manner. After treatment with 8 mM lidocaine, DJ‐1, and nuclear Nrf2, as well as antioxidative stress‐related proteins, GPx, GR, HO‐1, and CAT, were all significantly inhibited. Overexpression of DJ‐1 suppressed lidocaine‐induced apoptosis and oxidative stress in SH‐SY5Y cells and activated Nrf2 signalling at the same time, and these effects were reversed by the inhibition of Nrf2. DJ‐1 could protect SH‐SY5Y cells from lidocaine‐induced apoptosis through inhibition of oxidative stress via Nrf2 signaling.
Assimilation and short‐term processing of microphytobenthos nitrogen in intertidal sediments
Authors: Oakes Joanne M. , Riekenberg Philip M. , Eyre Bradley D. 
Published: May 2020

AbstractMicrophytobenthos (MPB) are an important nitrogen (N) sink in coastal systems, but little is known about the fate of N assimilated by MPB. We used an in situ 15N pulse‐chase experiment in intertidal, nonpermeable, sandy mud to trace the assimilation, transformation, and loss from the sediment of MPB‐N over 31 d. Following assimilation, 15N was tightly retained in surface sediments, with only up to 8.1% transported to 2–10 cm sediments over 31 d. MPB accounted for a considerable portion of the 15N in surface sediments throughout the study (59% ± 13%). Bacteria rapidly assimilated 15N but accounted for only up to 17% of the 15N within surface sediments. Of the assimilated 15N, 78.9% was lost from the sediment over 31 d. Resuspension was the dominant loss pathway (74.4%) and was primarily associated with minor flooding following a rainfall event at 8 d. Biological pathways for N export were far less important. Denitrification, during both sediment exposure and inundation, was the main biological pathway for 15N loss (2.6% total), whereas there was little loss through ammonification and nitrification (NH4+ or NOx efflux; 1.4% combined) and dissolved organic nitrogen efflux (0.5%). It was estimated that the N incorporated by MPB within one emersion period would take ~ 40 d to be entirely removed from the sediment. This study highlights the potential importance of MPB and intertidal sediments for the uptake and longer‐term storage of coastal N, and the need to better quantify the impact of episodic flooding on coastal N budgets.
Errors associated with compound‐specific δ15N analysis of amino acids in preserved fish samples purified by high‐pressure liquid chromatography
Authors: Swalethorp Rasmus , Aluwihare Lihini , Thompson Andrew R. , Ohman Mark D. , Landry Michael R. 
Published: May 2020

AbstractCompound‐specific isotopic analysis of amino acids (CSIA‐AA) is increasingly used in ecological and biogeochemical studies tracking the origin and fate of nitrogen (N). Its advantages include the potential for resolving finer‐scale trophic dynamics than possible with standard bulk SIA and for reconstructing historical changes in the food webs of consumers from analyses of specimens in preserved sample archives. For the latter, assessing the effects of chemical preservatives on δ15NAA has been inconclusive because the conventional CSIA approach for derivatized AAs by gas chromatography‐combustion‐isotope ratio mass spectrometry (GC‐C‐IRMS) has analytical errors (0.4–1.0‰) in the range expected from chemical preservation. Here, we show improved analytical precision (0.15 ± 0.08‰) for 11 underivatized AA standards analyzed by high‐pressure liquid chromatography followed by offline elemental analysis‐IRMS (HPLC/EA‐IRMS). Using this method, we report the first high‐precision tests of preservation effects on δ15NAA in Northern Anchovy (Engraulis mordax) kept 1.5‐yr in ethanol and up to 27‐yr in formaldehyde. We found minimal methodological induced fractionation for eight AAs, and preservation effects on δ15N were similar regardless of duration and preservative used. Although some of the AAs differed significantly from frozen control samples (AA average +1.0 ± 0.8‰), changes in δ15N in the source AA phenylalanine and trophic position estimates were statistically insignificant. Our results are encouraging for resolving fine‐scale natural variability using HPLC/EA‐IRMS on chemically preserved specimens and for ultimately reconstructing biogeochemical records and trophic dynamics over long time scales.
The interplay between cancer associated fibroblasts and immune cells in the context of radiation therapy
Authors: Piper Miles , Mueller Adam C. , Karam Sana D. 
Published: May 2020

AbstractFibroblasts are a key component of the tumor microenvironment (TME) that can serve as a scaffold for tumor cell migration and augment the tumor's ability to withstand harsh conditions. When activated by external or endogenous stimuli, normal fibroblasts become cancer associated fibroblasts (CAFs), a heterogeneous group of stromal cells in the tumor that are phenotypically and epigenetically different from normal fibroblasts. Dynamic crosstalk between cancer cells, immune cells, and CAFs through chemokines and surface signaling makes the TME conducive to tumor growth. When activated, CAFs promote tumorigenesis and metastasis through several phenomena including regulation of tumor immunity, metabolic reprogramming of the TME, extracellular matrix remodeling and contraction, and induction of therapeutic resistance. Ionizing radiation (radiation theraphy [RT]) is a potent immunological stimulant that has been shown to increase cytotoxic Teff infiltration and IFN‐I stimulated genes. RT, however, is unable to overcome the infiltration and activation of immunosuppressive cells which can contribute to tumor progression. Another paradox of RT is that, while very effective at killing cancer cells, it can contribute to the formation of CAFs. This review examines how the interplay between CAFs and immune cells during RT contributes to organ fibrosis, immunosuppression, and tumor growth. We focus on targeting mechanistic pathways of CAF formation as a potentially effective strategy not only for preventing organ fibrosis, but also in hampering tumor progression in response to RT.
An electronically switchable UWB to narrow band antenna for cognitive radio applications
Authors: Jacob Naveen , Kulkarni Muralidhar , K. Krishnamoorthy 
Published: May 2020

AbstractCognitive radio (CR) is designed to achieve intelligent spectrum sensing and sharing, in order to solve spectrum underutilization problem. Conventional CR uses multiple/multiport antenna to switch between UWB and narrow band (NB) frequencies for primary and secondary users. In this article, we propose a compact frequency reconfigurable single port antenna using PIN diodes, to switch between UWB and NB frequencies. A circular disc monopole antenna with partial ground plane is designed to deliver UWB response. Three interdigital capacitors (IDC) are electrically connected to a partial ground plane and feed line using PIN diodes. OFF condition of all PIN diodes will provide the UWB response of 2.8 to 10.6 GHz and ON condition of the diodes will result in 36 different NB frequencies, completely covering the UWB spectrum. A simulation study carried out by incorporating a varactor diode into IDC, clearly shows that, the antenna resonance can be switched using PIN diodes and each resonant frequency can be fine‐tuned independently, with in the band of interest. This is the unique feature of proposed antenna, which facilitates the technology for CR based internet of things applications in 5G wireless communication networks.
Lead‐207 NMR spectroscopy at 1.4 T: Application of benchtop instrumentation to a challenging I = ½ nucleus
Authors: Bernard Guy M. , Michaelis Vladimir K. 
Published: May 2020

AbstractThe practicality of obtaining liquid‐ and solid‐state 207Pb nuclear magnetic resonance (NMR) spectra with a low permanent‐field magnet is investigated. Obtaining 207Pb NMR spectra of salts in solution is shown to be viable for samples as dilute as 0.05 M. The concentration dependence of the 207Pb chemical shifts for lead nitrate was investigated; the results are comparable with those obtained with high‐field instruments. Likewise, the isotope effect of substituting D2O for H2O as the solvent was investigated and found to be comparable with those reported previously. Obtaining solid‐state 207Pb NMR spectra is challenging, but we demonstrate the ability to obtain such spectra for three unique solid samples. An axially symmetric 207Pb powder pattern for lead nitrate and the powder pattern expected for lead chloride reveal linewidths dominated by shielding anisotropy, while 207Pb‐35/37Cl J‐coupling dominates in the methylammonium lead chloride perovskite material. Finally, recent innovations and the future potential of the instruments are considered.