bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non‐coding RNA colon cancer‐associated transcript‐1 regulates tumor cell proliferation and invasion of non‐small‐cell lung cancer through suppressing miR‐152
Authors: Li Na , Hao Weiwei , Yang Junfang , Guo Yali , Guo Yonggang , Du Ying 
Published: March 2020

AimLung cancer serves as one of the most common cancers in the world, and approximately 50% of non‐small‐cell lung cancer (NSCLC) patients are found to be aged >70 when diagnosed. In this study, we aimed to explore the effect of long non‐coding RNAs colon cancer‐associated transcript‐1 (CCAT1) in NSCLC.MethodsA total of 72 clinical samples from older NSCLC patients were collected for analysis. The relative mRNA level of CCAT1 was detected through real‐time polymerase chain reaction. Overall survival of NSCLC patients was detected through Kaplan–Meier survival analysis. MTT assays were used to detect cell proliferation. Cell invasion was determined by transwell assay. Protein levels were detected through western blot.ResultsCCAT1 expression levels significantly increased in NSCLC tumor tissues and were associated with poor overall survival of NSCLC patients. CCAT1 promotes cell proliferation, cell invasion and epithelial–mesenchymal transition of NSCLC cell lines. CCAT1 binds with miR‐152, and the effect of si‐CCAT1 in NSCLC cell proliferation, cell invasion and epithelial–mesenchymal transition was partially reversed by anti‐miR‐152.ConclusionsLong non‐coding RNA CCAT1 regulates tumor cell proliferation and invasion in NSCLC through suppressing miR‐152. Geriatr Gerontol Int 2020; ••: ••–••.
Migraine Care in the Era of COVID‐19: Clinical Pearls and Plea to Insurers
Authors: Szperka Christina L. , Ailani Jessica , Barmherzig Rebecca , Klein Brad C. , Minen Mia T. , Halker Singh Rashmi B. , Shapiro Robert E. 
Published: March 2020

ObjectiveTo outline strategies for the treatment of migraine which do not require in‐person visits to clinic or the emergency department, and to describe ways that health insurance companies can remove barriers to quality care for migraine.BackgroundCOVID‐19 is a global pandemic causing widespread infections and death. To control the spread of infection we are called to observe “social distancing” and we have been asked to postpone any procedures which are not essential. Since procedural therapies are a mainstay of headache care, the inability to do procedures could negatively affect our patients with migraine. In this manuscript we review alternative therapies, with particular attention to those which may be contra‐indicated in the setting of COVID‐19 infection.Design/ResultsThe manuscript reviews the use of telemedicine visits and acute, bridge, and preventive therapies for migraine. We focus on evidence‐based treatment where possible, but also describe “real world” strategies which may be tried. In each section we call out areas where changes to rules from commercial health insurance companies would facilitate better migraine care.ConclusionsOur common goal as health care providers is to maximize the health and safety of our patients. Successful management of migraine with avoidance of in‐person clinic and emergency department visits further benefits the current urgent societal goal of maintaining social distance to contain the COVID‐19 pandemic.
Spectrum of skin diseases in Amerindian villages of the Upper Oyapock, French Guiana
Authors: Malmontet Thomas , Guarmit Basma , Gaillet Melanie , Michaud Celine , Garceran Nicolas , Chanlin Romain , Demar Magalie , Couppie Pierre , Blaizot Romain 
Published: March 2020

AbstractBackgroundDue to their genetic characteristics, their isolation in rainforest areas, and their traditional way of life, Amerindian populations are likely to suffer from a specific spectrum of dermatoses. However, there are few available data on such skin disorders. Our aims were to describe all skin disorders in two Amerindian villages of French Guiana.MethodsThis retrospective study concerned all patients who consulted in the Health Centres of Camopi and Trois‐Sauts between July 1, 2017, and December 31, 2018. We included all patients classified with an ICD code linked to a skin disorder. All medical records were cross‐checked by two dermatologists to correct misclassifications.ResultsA total of 639 patients formed the study population, for 866 different skin disorders. Non‐sexually transmitted infections represented 57.6% of all skin disorders, followed by eczema (11.5%) and bites/envenomations (9.1%). Bacteria were responsible for 238 skin infections, followed by fungi (141 cases) and parasites (69 cases, including 43 scabies, nine cutaneous leishmaniasis, and two tungiasis). We reported a low prevalence of sexually transmitted infections (10 cases) and an absence of skin cancers.ConclusionsThis study revealed the absence of skin cancer in the Amerindian population of the Upper Oyapock and the important burden of infectious and animal‐related diseases. Future studies should assess a possible underestimation of sexually transmitted diseases in this area. Public health policies should target neglected diseases such as cutaneous leishmaniasis, tungiasis, scabies, and envenomations. Atopic dermatitis was a significant and unexpected cause of consultations.
Controllable synthesis of Eu3+‐doped Y2O3 nanocrystal/g‐C3N4 composites with tunable fluorescence
Authors: Zheng Zekun , Zeng Zixin , Guo Qianyi , Pan Qiwen , Qiu Jianrong , Dong Guoping 
Published: March 2020

ABSTRACTThe application of graphitic carbon nitride (g‐C3N4) has been restricted in some optoelectronics fields due to its narrow tunable emission region. In order to engineer the optical properties, herein we combine Eu3+:Y2O3 nanocrystals with g‐C3N4to control its electronic structure. Surprisingly, with the increasing concentration of Eu3+:Y2O3 nanocrystals, the photoluminescence of Eu3+:Y2O3/g‐C3N4 (EYCN) showed a continuous red shift in comparison with pure g‐C3N4 from blue to green. In EYCN composites, the Eu3+:Y2O3 nanocrystals as electron donors, can improve the degree of electron delocalization and narrow the bandgap. Importantly, the EYCN composites not only maintain the intrinsic blue‐green emission of g‐C3N4, but also introduce the red light of Eu3+ ions. Based on the EYCN composites, white light‐emitting diode (WLED) were fabricated with a Commission International de L’Eclairage value of (0.3631, 0.3560). This work proposes a new kind of luminescent g‐C3N4‐based composites, which is expected to broaden its applications in optoelectronics field.
FGF21 induces autophagy‐mediated cholesterol efflux to inhibit atherogenesis via RACK1 up‐regulation
Authors: Xiaolong Lin , Dongmin Guo , Liu Mihua , Zuo Wang , Huijun Hu , Qiufen Tan , XueMei Hu , Wensheng Lin , Yuping Pan , Jun Lin , Zhaolin Zeng 
Published: March 2020

AbstractFibroblast growth factor 21 (FGF21) acts as an anti‐atherosclerotic agent. However, the specific mechanisms governing this regulatory activity are unclear. Autophagy is a highly conserved cell stress response which regulates atherosclerosis (AS) by reducing lipid droplet degradation in foam cells. We sought to assess whether FGF21 could inhibit AS by regulating cholesterol metabolism in foam cells via autophagy and to elucidate the underlying molecular mechanisms. In this study, ApoE−/− mice were fed a high‐fat diet (HFD) with or without FGF21 and FGF21 + 3‐Methyladenine (3MA) for 12 weeks. Our results showed that FGF21 inhibited AS in HFD‐fed ApoE−/− mice, which was reversed by 3MA treatment. Moreover, FGF21 increased plaque RACK1 and autophagy‐related protein (LC3 and beclin‐1) expression in ApoE−/− mice, thus preventing AS. However, these proteins were inhibited by LV‐RACK1 shRNA injection. Foam cell development is a crucial determinant of AS, and cholesterol efflux from foam cells represents an important defensive measure of AS. In this study, foam cells were treated with FGF21 for 24 hours after a pre‐treatment with 3MA, ATG5 siRNA or RACK1 siRNA. Our results indicated that FGF21‐induced autophagy promoted cholesterol efflux to reduce cholesterol accumulation in foam cells by up‐regulating RACK1 expression. Interestingly, immunoprecipitation results showed that RACK1 was able to activate AMPK and interact with ATG5. Taken together, our results indicated that FGF21 induces autophagy to promote cholesterol efflux and reduce cholesterol accumulation in foam cells through RACK1‐mediated AMPK activation and ATG5 interaction. These results provided new insights into the molecular mechanisms of FGF21 in the treatment of AS.
A comprehensive analysis of IDO1 expression with tumour‐infiltrating immune cells and mutation burden in gynaecologic and breast cancers
Authors: Feng Xu , Tang Ranran , Zhang Runjie , Wang Huan , Ji Zhaodong , Shao Yang , Wang Shuoer , Zhong Tianying , Gu Yun , Meng Jiao 
Published: March 2020

AbstractGynaecologic and breast cancers share some similarities at the molecular level. The aims of our study are to highlight the similarities and differences about IDO1, an important immune‐related gene in female cancers. The NGS data from TCGA of cervical squamous cell carcinoma (CESC), ovarian serous cystadenocarcinoma (OV), uterine corpus endometrial carcinoma (UCEC), uterine carcinosarcoma (UCS) and breast invasive carcinoma (BRCA) were analysed to identify molecular features, and clinically significant and potential therapeutic targets of IDO1. We found IDO1 was significantly up‐regulated in four gynaecologic cancers and breast cancer. According to breast cancer PAM50 classification scheme, IDO1 expression was higher in tumours of basal than other subtypes and showed better survival prognosis in BRCA and OV. Through immune infiltration analysis, we found a strong correlation between IDO1 and immune cell populations especially for dendritic cells and T cells. In addition, we investigated the association between IDO1 and tumour mutation burden (TMB) and found that IDO1 was significantly correlated with TMB in BRCA and CESC. GSVA revealed that hallmarks significantly correlated with IDO1 were involved in interferon gamma response, allograft rejection and inflammatory response. We also found PD‐L1 and LAG3 were highly positive related to IDO1 in gynaecologic cancers when comparing with their corresponding normal tissues. Our results indicated that IDO1 participated in anti‐tumour immune process and is correlated with mutation burden. These findings may expand our outlook of potential anti‐IDO1 treatments.
Overexpression of peptidase inhibitor 16 attenuates angiotensin II–induced cardiac fibrosis via regulating HDAC1 of cardiac fibroblasts
Authors: Deng Mengqing , Yang Shuo , Ji Yue , Lu Yan , Qiu Ming , Sheng Yanhui , Sun Wei , Kong Xiangqing 
Published: March 2020

AbstractCardiac hypertrophy and fibrosis are the major causes of heart failure due to non‐ischaemia heart disease. To date, no specific therapy exists for cardiac fibrosis due to the largely unknown mechanisms of disease and lack of applicable therapeutic targets. In this study, we aimed to explore the role and associated mechanism of peptidase inhibitor 16 (PI16) in cardiac fibrosis induced by angiotensin II. In cardiac fibroblasts (CFs), overexpressed PI16 significantly inhibited CF proliferation and the levels of fibrosis‐associated proteins. Further analysis of epigenetic changes in CF revealed that overexpressed PI16 decreases the nuclear level of histone deacetylase 1 (HDAC1) after angiotensin II treatment, resulting in increased histone 3 acetylation in K18 and K27 lysine. However, overexpression of HDAC1 by an adenovirus vector in CFs reversed these changes. Echocardiography showed that PI16 transgenic (Tg) mice have smaller left ventricle mass than wild‐type mice. Histological analysis data showed that PI16 Tg mice demonstrated smaller cardiomyocyte size and less collagen deposition than wild‐type mice. The effects of PI16 on HDAC1 and histone 3 were also confirmed in PI16 Tg mice using immunostaining. Generally, PI16 is a HDAC1 regulator specifically in CFs, and PI16 overexpression prevents cardiac hypertrophy and fibrosis by inhibiting stress‐induced CF activation.
Association between family history of suicide attempt and neurocognitive functioning in community youth
Authors: Jones Jason D. , Boyd Rhonda C. , Calkins Monica E. , Moore Tyler M. , Ahmed Annisa , Barzilay Ran , Benton Tami D. , Gur Raquel E. , Gur Ruben C. 
Published: March 2020

BackgroundSuicidal behavior is highly familial. Neurocognitive deficits have been proposed as an endophenotype for suicide risk that may contribute to the familial transmission of suicide. Yet, there is a lack of research on the neurocognitive functioning of first‐degree biological relatives of suicide attempters. The aim of the present study is to conduct the largest investigation to date of neurocognitive functioning in community youth with a family history of a fatal or nonfatal suicide attempt (FH).MethodsParticipants aged 8–21 years from the Philadelphia Neurodevelopmental Cohort completed detailed clinical and neurocognitive evaluations. A subsample of 501 participants with a FH was matched to a comparison group of 3,006 participants without a family history of suicide attempt (no‐FH) on age, sex, race, and lifetime depression.ResultsAfter adjusting for multiple comparisons and including relevant clinical and demographic covariates, youth with a FH had significantly lower executive function factor scores (F[1,3432] = 6.63, p = .010) and performed worse on individual tests of attention (F[1,3382] = 7.08, p = .008) and language reasoning (F[1,3387] = 5.12, p = .024) than no‐FH youth.ConclusionsYouth with a FH show small differences in executive function, attention, and language reasoning compared to youth without a FH. Further research is warranted to investigate neurocognitive functioning as an endophenotype for suicide risk. Implications for the prevention and treatment of suicidal behaviors are discussed.
Drug‐Eluting Balloon Angioplasty for In‐Stent Restenosis Following Carotid Artery Stent Placement
Authors: Bhatia Kunal , Akhtar Iqra N. , Akinci Yasemin , Liaqat Jahanzeb , Siddiq Farhan , Gomez Camilo R. , Qureshi Adnan I. 
Published: March 2020

ABSTRACTBACKGROUND AND PURPOSEDrug‐eluting balloons (DEBs) have been proposed as an option for the treatment of in‐stent restenosis (ISR) following carotid artery stent placement. We report our experience and review of literature to provide additional data.METHODSFor literature review, PubMed search was conducted to identify studies published between 2005 and 2019, reporting data on management of carotid ISR with DEBs. Two cases with carotid ISR, which were successfully treated with DEB at our facility, were also included in the final compilation of resultsRESULTSA total of seven studies demonstrating the use of the DEBs for treatment of carotid ISR were identified. They encompassed 31 patients, 11 (35.5%) of whom presented with symptomatic ISR, with the remaining 20 patients (64.5%) asymptomatic. DEB angioplasty followed by stent placement was performed in 3 patients, whereas DEB alone was utilized in 28 patients. Periprocedural complications included asymptomatic dissection from DEB inflation in 1 patient and transient neurological deficits in another patient. Follow‐up period was variable and ranged from 1 month to 5 years. Three patients were noted to develop recurrent asymptomatic stenosis, whereas 1 patient developed an episode of symptomatic restenosis post procedural on follow‐up. In our two cases, both patients were noted to have protracted period of hypotension postprocedure without any new or recurrent neurological symptoms.CONCLUSIONThe use of DEBs is a promising development and a viable alternative for management of severe and recurrent carotid ISR.
Pharmaceutical evaluation of matrix tablets prepared using a fused deposition modelling type three-dimensional printer – Effect of geometrical internal microstructural factors on drug release from enteric-polymer tablets containing rebamipide
Authors: Hattori Yusuke , Kubota Shiho , Otsuka Makoto 
Published: March 2020

Abstract

Objectives
Three-dimensional (3-D) printers are widely expected to provide a novel manufacturing method in the future to make personalized medicines in hospitals.

Methods
Functional filament containing 5–20% drug was prepared with rebamipide (REB; a poorly water-soluble model drug) and hypromellose phthalate (HPMCP; enteric base) at 130–170°C using a biaxial kneading extruder. Several tablet models with various internal structures were designed using computer-aided design. REB-containing 3-D tablets were prepared based on these designs from filament using a fused deposition modelling type 3-D printer.

Key findings
Physicochemical characteristics of the filament and 3-D tablets were measured by powder X-ray diffraction and differential scanning calorimetry. The results suggested that a part of the crystalline REB dispersed into the HPMCP and transformed into an amorphous form, because crystalline REB was kneaded with melted HPMCP at 130–170°C during the preparation processes of filament and 3-D tablets. The drug-release properties of 3-D tablets were tested in both pH 1.2 and 6.8 buffers. REB was not released from the 3-D tablets at pH 1.2, but HPMCP dissolved at pH 6.8, and then REB was rapidly released from the tablet.

Conclusions
The dissolution of 3-D tablets in the small intestine could be controlled by the tablet geometrical structure.
Evaluation of terrestrial carbonaceous matter aromatization by Raman spectroscopy and its application to C chondrites
Authors: Schmidt Jaques S. , Hinrichs Ruth 
Published: March 2020

AbstractA Raman method to estimate the aromatization degree of carbonaceous matter (CM) was established, and calibrated by literature data respecting differences in acquisition procedures. The generated equations allowed a comparison of the G‐band width to vitrinite reflectance, and to additional structural organization parameter extracted from X‐ray powder diffraction. The relations between aromatization degree and peak temperatures from literature were then propagated to G‐band width parameter. The applicability of these geothermometers is demonstrated by the consistency of data obtained for carbonaceous chondrites with previous estimations. Since the CM aromatization is controlled by peak temperature and time span, it is possible to estimate the duration of chondrite’s heating events. The G‐band width relation to elemental ratio trends of organic matter in chondrites, interplanetary dust particles, glucose, and saccharose‐based semi‐coke favors synthesis by formose route. These findings provide new approaches for the future development of chemical kinetic models for organic matter of chondrites. A reliable chemical model may allow the external calibration of numerical models for accurately evaluating the peak temperatures and cooling durations of chondrites.
Parentage analyses identify local dispersal events and sibling aggregations in a natural population of Millepora hydrocorals, a free‐spawning marine invertebrate
Authors: Dubé Caroline E. , Boissin Emilie , Mercière Alexandre , Planes Serge 
Published: March 2020

AbstractDispersal is a critical process for the persistence and productivity of marine populations. For many reef species, there is increasing evidence that local demography and self‐recruitment have major consequences on their genetic diversity and adaptation to environmental change. Yet empirical data of dispersal patterns in reef‐building species remain scarce. Here, we document the first genetic estimates of self‐recruitment and dispersal distances in a free‐spawning marine invertebrate, the hydrocoral Millepora cf. platyphylla. Using twelve microsatellite markers, we gathered genotypic information from 3,160 georeferenced colonies collected over 27,000 m2 of a single reef in three adjacent habitats in Moorea, French Polynesia; the mid slope, upper slope, and back reef. Although the adult population was predominantly clonal (85% were clones), our parentage analysis revealed a moderate self‐recruitment rate with a minimum of 8% of sexual propagules produced locally. Assigned offspring often settled at <10 m from their parents and dispersal events decrease with increasing geographic distance. There were no discrepancies between the dispersal distances of offspring assigned to parents belonging to clonal versus nonclonal genotypes. Interhabitat dispersal events via cross‐reef transport were also detected for sexual and asexual propagules. Sibship analysis showed that full siblings recruit nearby on the reef (more than 40% settled at <30 m), resulting in sibling aggregations. Our findings highlight the importance of self‐recruitment together with clonality in stabilizing population dynamics, which may ultimately enhance local sustainability and resilience to disturbance.
Patience is not always a virtue: effects of terrain complexity on the host‐seeking behaviour of adult blacklegged ticks, Ixodes scapularis, in the presence of a stationary host
Authors: Curtis T. R. , Shi M. , Qiao X. 
Published: March 2020

AbstractBlacklegged ticks, Ixodes scapularis Say (Acari: Ixodidae), are the primary vectors of Lyme disease in the U.S.A. In this study, adult ticks were observed on public trails exhibiting increasing levels of terrain complexity with a potential host nearby. The goal of this study was to (a) examine the extent to which adult ticks may actively search (vs. sit‐and‐wait) for a nearby host, (b) determine whether or not ticks could locate the position of the host in natural conditions and (c) determine the role of terrain complexity on the distances ticks travelled in a short period of time (30 min). Results indicate that, when a potential stationary host is within 50 cm, ticks will utilize an active‐search strategy. The majority of ticks moved in the direction of the host in natural conditions. Finally, ticks in a less complex terrain were more active and travelled greater horizontal distances than ticks in a more complex terrain. In conclusion, the use of an active‐search approach would likely increase the foraging success of ticks, especially in terrains with minimal complexity, near host animals that have stopped to rest or feed, reinforcing that humans should be vigilant about checking for ticks after being outdoors.
STONE ‘CANVAS’ AND NATUFIAN ART: AN INCISED HUMAN FIGURE FROM THE NATUFIAN CEMETERY AT RAQEFET CAVE, ISRAEL
Authors: Rosenberg Danny , Chasan Rivka , Lengyel György , Nadel Dani 
Published: March 2020

SummaryThe Natufian culture is the first cultural entity in the southern Levant to have large graveyards and a wide array of symbolic expressions. Hundreds of Natufian burials have been discovered and studied; at some Natufian sites the presence of stone slabs within the graves has been recorded. One interesting yet rare phenomenon documented is the slabs bearing clear incised patterns, suggesting the Natufians were using these flat stone blocks as 'canvases'. Here we report and discuss an incised anthropomorphic figure that has been discovered in the Natufian graveyard at Raqefet Cave, Mt. Carmel, Israel. The human figure, most likely shown as dancing, adds another dimension to the rich assemblage of Natufian burial practices and symbolism as recorded at the site of Raqefet Cave as well as at other Natufian sites.