bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leucine rich repeats and calponin homology domain containing 1 inhibits microglia-mediated neuroinflammation in a rat traumatic spinal cord injury model
Authors: Chen Wen-Kai , Feng Lin-Juan , Liu Qiao-Dan , Ke Qing-Feng , Cai Pei-Ya , Zhang Pei-Ru , Cai Li-Quan , Huang Nian-Lai , Lin Wen-Ping 
Published: December 2019

Abstract
Background Spinal cord injury (SCI) triggers the primary mechanical injury and secondary inflammation-mediated injury. Neuroinflammation-mediated insult causes secondary and extensive neurological damage after SCI. Microglia play a pivotal role in the initiation and progression of post-SCI neuroinflammation.Methods To elucidate the significance of LRCH1 to microglial functions, we applied lentivirus-induced LRCH1 knockdown in primary microglia culture, and tested the role of LRCH1 in microglia-mediated inflammatory reaction both in vitro and in a rat SCI model.ResultsWe found that LRCH1 was down-regulated in microglia after traumatic SCI. LRCH1 knockdown increased the production of pro-inflammatory cytokines such as IL-1β, TNF-α, and IL-6 after in vitro priming with lipopolysaccharide and adenosine triphosphate. Furthermore, LRCH1 knockdown promoted the priming-induced microglial polarization towards the pro-inflammatory M1 type, as demonstrated by increased differentiation into inducible nitric oxide synthase (iNOS)+ microglia. LRCH1 knockdown also enhanced microglia-mediated N27 neuron death after priming. Further analysis revealed that LRCH1 knockdown increased priming-induced activation of p38 mitogen-activated protein kinase (MAPK) and Erk1/2 signaling, which are crucial for M1 polarization of microglia. When LRCH1-knockdown microglia were adoptively injected into rat spinal cords, they enhanced post-SCI production of pro-inflammatory cytokines, increased SCI-induced recruitment of leukocytes, aggravated SCI-induced tissue damage and neuronal death, and worsened the locomotor function.Conclusion Our study reveals for the first time that LRCH1 serves as a negative regulator of microglia-mediated neuroinflammation after SCI, and provides clues for developing novel therapeutic approaches against SCI.
Characterization of astrocytes throughout life in wildtype and APP/PS1 mice after early-life stress exposure
Authors: Abbink Maralinde R. , Kotah Janssen M. , Hoeijmakers Lianne , Mak Aline , Yvon-Durocher Genevieve , Gaag Bram van der , Lucassen Paul J. , Korosi Aniko 
Published: December 2019

Abstract
Background Early-life stress (ES) is an emerging risk factor for later-life development of Alzheimer’s disease (AD). We have previously shown that ES modulates amyloid-beta pathology and the microglial response to it in the APPswe/PS1dE9 mouse model. Because astrocytes are key players in the pathogenesis of AD, we studied here if and how ES affects astrocytes in wildtype (WT) and APP/PS1 mice, and how these relate to the previously reported amyloid pathology and microglial profile.Methods We induced ES by limiting nesting and bedding material from postnatal days (P) 2-9. We studied in WT mice (at P9, P30 and 6 months) and in APP/PS1 mice (at 4 and 10 months) i) GFAP coverage, cell density and complexity in hippocampus (HPC) and entorhinal cortex (EC); ii) hippocampal gene expression of astrocyte markers; and iii) the relationship between astrocyte, microglia and amyloid markers.ResultsIn WT mice, ES increased GFAP coverage in HPC subregions at P9, and decreased it at 10 months. APP/PS1 mice at 10 months exhibited both individual cell as well as clustered GFAP signals. APP/PS1 mice when compared to WT exhibited reduced total GFAP coverage in HPC, which is increased in the EC, while coverage of the clustered GFAP signal in the HPC was increased and accompanied by increased expression of several astrocytic genes. While measured astrocytic parameters in APP/PS1 mice appear not be further modulated by ES, analyzing these in the context of ES-induced alterations to amyloid pathology and microglial shows alterations at both 4 and 10 months of age.Conclusions Our data suggest that ES leads to alterations to the astrocytic response to amyloid-β pathology.
Identification and Evolutionary Analysis of the Nucleolar Proteome of Giardia lamblia
Authors: Jin-mei Feng , Chun-Lin Yang , Hai-Feng Tian , Jiang-Xin Wang , Wen Jianfan 
Published: December 2019

Abstract
Background: The nucleoli, including their proteomes, of higher eukaryotes have been extensively studied, while few studies about the nucleoli of the lower eukaryotes – protists were reported. Giardia lamblia, a protist with the controversy of whether it is an extreme primitive eukaryote or just a highly evolved parasite, might be an interesting object for carrying out the nucleolar proteome study of protists and further examining the controversy.Results: Using bioinformatics methods, we reconstructed G. lamblia nucleolar proteome (GiNuP) and the common nucleolar proteome of the three higher eukaryotes (human, Arabidopsis, yeast) (HEBNuP). Comparisons of the two proteomes revealed that: 1) GiNuP is much smaller than HEBNuP, but 78.4% of its proteins have orthologs in the latter; 2) More than 68% of the proteins in GiNuP are involved in the “Ribosome related” function, and the others participate in the other functions, and these two groups of proteins are much larger and much smaller than those in HEBNuP, respectively; 3) Both GiNuP and HEBNuP have their own specific proteins, but HEBNuP has a much higher proportion of such proteins to participate in more categories of functions.Conclusion: For the first time the nucleolar proteome of a protist - Giardia was reconstructed. The results of comparison of it with the common proteome of three representative higher eukaryotes -- HEBNuP indicated that the relatively simple GiNuP should reflect the primitiveness but not the parasitic reduction of Giardia, and simultaneously revealed some interesting evolutionary phenomena about the nucleolus and even the eukaryotic cell, compositionally and functionally.
Transcriptome analysis of Spodoptera litura reveals the molecular mechanism to pyrethroids resistance
Authors: Li Dongzhi , Mei Yu , Liu Runqiang , Chen Xiling , Xu Li , Wang Chengju 
Published: December 2019

Abstract
Background Spodoptera litura is a destructive agricultural pest and has evolved resistance to multiple insecticides, especially pyrethroids. At present, the resistance mechanism to pyrethroids remains unclear.Results Four field-collected populations, namely CZ, LF, NJ and JD, were identified to have high resistance to pyrethroids comparing to pyrethroid-susceptible population (GX), with increased resistant ratio ranging from 40.8 to 1764.0-fold. To characterize pyrethroid resistance mechanism, the transcriptomes between pyrethroid-resistant (LF and NJ) and pyrethroid-susceptible (GX) populations were compared by RNA-sequencing. Results showed that multiple differentially expressed genes were enriched in metabolism-related GO terms and KEGG pathways. 35 up-regulated metabolism-related genes were screened to verify by qRT-PCR. Consistent up-regulation of 13 unigenes, including 3 P450s, 4 GSTs, 1 UGT, 4 COEs and 1ABC, were verified in the additional pyrethroid resistant populations CZ and JD. The expression levels of CYP3 and GST3 , which were homologous to CYP321A8 and GST1 , respectively, showed positive correlation with their pyrethroid resistance levels within CZ, LF, NJ and JD populations. While the expression levels of CYP12 , CYP14 , COE4 and ABC5 showed good correlation with their pyrethroid resistance levels in at least three populations. UGT5 had the highest expression level among the tested UGT genes in the four pyrethroid resistant populations.Conclusion CYP3 , CYP12 , CYP14 , GST3 , COE4 , UGT5 and ABC5 play important roles in pyrethroid resistance among the four field-collected populations. Our work provides a valuable clue for further study of pyrethroid resistance mechanisms in S. litura .
18F- FDG PET/CT-derived parameters predict clinical stage and prognosis of esophageal cancer.
Authors: Mantziari Styliani , Pomoni Anastasia , Prior John O , Winiker Michael , Allemann Pierre , Demartines Nicolas , Schäfer Markus 
Published: December 2019

Abstract
Background Although 18 F- FDG PET/CT is validated in baseline workup of esophageal cancer to detect distant metastases, it remains underused in assessing local staging and biology of the primary tumor. This study aimed to evaluate the association between 18 F- FDG PET/CT-derived parameters of esophageal cancer, and its clinico-pathological features and prognosis.Methods All patients (n=86) with esophageal adenocarcinoma or squamous cell cancer operated between 2005-2014 were analyzed. Linear regression was used to identify clinico-pathologic features of esophageal cancer associated with the tumor’s maximal Standardized Uptake Value (SUV max ), Total Lesion Glycolysis (TLG) and Metabolic Tumor Volume (MTV). ROC curve analysis was performed to precise the optimal cutoff of each variable associated with a locally advanced (cT3/4) status, long-term survival and recurrence. Kaplan Meier curves and Cox regression were used for survival analyses.Results High baseline SUV max was associated with cT3/4 status and middle-third tumor location, TLG with a cT3/4 and cN+ status, whereas MTV only with active smoking. A cT3/4 status was significantly predicted by a SUV max >8.25g/mL (p<0.001), TLG>41.7 (p<0.001) and MTV>10.70 cm 3 (p<0.01) whereas a SUV max > 12.7 g/mL was associated with an early tumor recurrence and a poor disease-free survival (median 13 versus 56 months, p=0.030), particularly in squamous cell cancer.Conclusions Baseline 18 F- FDG PET/CT has a high predictive value of preoperative cT stage, as its parameters SUV max , TLG and MTV can predict a locally advanced tumor with high accuracy. A SUV max > 12.7 g/mL may herald early tumor recurrence and poor disease-free survival.
Analysis of anesthesia-related prognostic indicators in neurosurgery
Authors: Shu Aihua , Wang Qiang , Chen Xiaobo , Zhou Feng , Liu Yifei 
Published: December 2019

Abstract
Background: This retrospective study was conducted to identify prognostic predictors for patients undergoing neurosurgery under general anesthesia.Methods: From April 2015 to May 2017, a total of 757 patients undergoing neurosurgery in our hospital were analyzed. Baseline and clinical data including age, gender, etiology, surgery procedures, comorbid and underlying diseases, the Glasgow coma scale (GCS), the APACHE II score, ASA score, anesthesia class, operation time, intraoperative cardiac events, intraoperative blood transfusion, and postoperative fever were collected and analyzed. The Glasgow outcome scale (GOS) was used as outcome measure. Logistic regression analysis was performed to identify the independent prognostic factors. Results: Among the 14 factors analyzed, twelve were significantly correlated to the GOS scores (P < 0.05), including age, etiology, type of surgery, hypertension, anesthesia class, APACHE II score and ASA class, intraoperative cardiac events, intraoperative blood transfusion and postoperative fever. Further logistic regression analysis showed the three parameters had prognostic values with odds ratios of 3.933, 1.812 and 2.910, respectively. On other hand, gender and operation time were not correlated to GOS.Conclusions: Anesthesia class, APACHE II score and ASA class have significant prognostic values for patients undergoing neurosurgical surgery.
Evaluation of peripheral perfusion index and heart rate variability as early predictors for intradialytic hypotension in critically ill patients.
Authors: Mostafa Hanan , Shaban Mohamed , Hasanin Ahmed , Mohamed Hassan , Fathy Shymaa , Abdelreheem Hossam M , Lotfy Ahmed , Abougabal Ayman , Mukhtar Ahmed , El-adawy Akram 
Published: December 2019

Abstract
Background Intradialytic hypotension is a serious complication during renal replacement therapy in critically ill patients. Early prediction of intradialytic hypotension could allow adequate prophylactic measures. In this study we evaluated the ability of peripheral perfusion index (PPI) and heart rate variability (HRV) to predict intradialytic hypotension. Methods A prospective observational study included 36 critically ill patients with acute kidney injury during their first session of intermittent hemodialysis. In addition to basic vital signs, PPI was measured using Radical-7 (Masimo) device. Electrical cardiometry (ICON) device was used for measuring cardiac output, systemic vascular resistance, and HRV. All hemodynamic values were recorded at the following time points: 30 minutes before the hemodialysis session, 15 minutes before the start of hemodialysis session, every 5 minutes during the session, and 15 minutes after the conclusion of the session. The ability of all variables to predict intradialytic hypotension was assessed through area under receiver operating characteristic (AUROC) curve calculation. Results Twenty-three patients (64%) had intradialytic hypotension. Patients with pulmonary oedema showed higher risk for development of intradialytic hypotension {Odds ratio (95% CI): 13.75(1.4-136)}. Each of baseline HRV, and baseline PPI showed good predictive properties for intradialytic hypotension {AUROC (95% CI): 0.761(0.59-0.88)}, and 0.721(0.547-0.857)} respectively. Conclusions Each of low PPI, low HRV, and the presence of pulmonary oedema are good predictors of intradialytic hypotension.
ESTIMATION OF INCOME CHARACTERISTICS FOR REGIONS IN POLAND USING SPATIO-TEMPORAL SMALL AREA MODELS
Authors: Jędrzejczak Alina , Kubacki Jan 
Published: December 2019

Abstract
The paper presents the comparison of estimation results for spatial and spatiotemporal small area models. The study was carried out for income-related variables drawn from the Polish Household Budget Survey and explanatory variables from the Polish Local Data Bank for the years 2003-2013. The properties of EBLUPs (Empirical Best Linear Unbiased Predictors) based on spatiotemporal models, which utilize spatial correlation between neighbouring areas as well as historical data, were compared and contrasted with EBLUPs based on spatial models obtained separately for each year and with EBLUPs based on the Rao-Yu model. The computations were performed using sae, sae2 and spdep packages for R-project environment. In the case of sae package, the eblupFH, eblupSFH and the eblupSTFH functions were used for point estimation along with the mseFH, mseSFH and the pbmseSTFH functions for the MSE estimation, whereas the eblupRY function was applied for the purposes of sae2 package. The precision of direct estimators was guaranteed by the adoption of the Balanced Repeated Replication method. The results of the analysis demonstrate that a visible reduction of the estimation error was achieved for the implemented spatiotemporal small-area models, especially when significant spatial and time autocorrelations were observed. These results are even more valuable than those achieved by the means of the Rao-Yu model. In the computations, three author-defined functions were adopted, which not only enabled the author to perform the extract of random effects for spatial, spatiotemporal and Rao-Yu models, but also made it possible to obtain their decomposition with respect to spatial and temporal parts, thus creating a novel solution. The comparison was carried out using choropleth maps for spatial effects and distributions of temporal random effects for the considered years.
Kemampuan Berfikir Kritis Matematis Siswa dengan Menggunakan Model Collaborative Learning dengan Pendekatan Open-Ended Berbantuan Media Macroflash 8
Authors: Janah Ika Nur , Kusumadewi Rida Fironika , Ulia Nuhyal 
Published: December 2019

Kemampuan berfikir kritis matematis merupakan kemampuan berfikir dalam menyelesaikan suatu masalah yang melibatkan pengetahuan, penalaran, dan pembuktian matematika. Namun pada kenyataannya kemampuan berfikir kritis matematis siswa kelas V SD Negari Sembungharjo 01 masih rendah. Tujuan dari pembelajaran ini adalah agar kemampuan berfikir kritis matematis siswa berkembang setelah pembelajaran dengan model <em>collaborative learning </em>berpendekatan <em>open-ended</em> berbantuan media <em>macroflash</em> 8. Penelitian ini menggunakan desain <em>Quasi Experimental Design </em>dengan bentuk <em>pretest-posttest control group design</em> Hasil penelitian menunjukkan bahwa: (1) kemampuan berfikir kritis matematis siswa kelas eksperimen menggunakan model <em>collaborative learning</em> berpendekatan <em>open-ended </em>berbantuan media <em>macroflash </em>8 lebih baik atau sama terhadap siswa kelas kontrol dengan menggunakan model ekspositori, dibuktikan dengan hasil -t tabel (-2,034) ≤ t hitung (1,503) ≤ t hitung (2,034) sehingga Ho ditolak dan Ha diterima diartikan terdapat perbandingan nilai <em>pretest</em> dan <em>posttest</em> pada kelas eksperimen dan kelas kontrol pada uji-t. Dalam penelitian ini dapat disimpulkan bahwa setelah diberikan pembelajaran model <em>collaborative learning</em> berpendekatan <em>open-ended</em> terdapat perbedaan hasil dari sebelum dan sesudah pembelajaran terhadap kemampuan berfikir kritis matematis.
Cell-Derived Microparticles and Acute Coronary Syndromes: Is there a Predictive Role for Microparticles?
Authors: Zacharia Effimia , Zacharias Konstantinos , Papamikroulis George-Angelo , Bertsias Dimitrios , Miliou Antigoni , Pallantza Zoi , Papageorgiou Nikolaos , Tousoulis Dimitris 
Published: December 2019

Background:Despite the recent advances in the treatment of Acute Coronary Syndromes (ACS), patients with ACS are still exposed to an increased risk for adverse cardiovascular events, while their prognosis is difficult to determine. Experimental and clinical studies have shown that cell-derived Microparticles (MPs) are associated with the underlying pathophysiological processes that are responsible for atherogenesis and may be causally implicated in the induction of atherothrombosis.Objective:In the present article, we aimed to review the available evidence regarding the predictive role of MPs in patients with ACS.Results:Evidence suggests that endothelial MPs are associated with future adverse cardiovascular events in patients with ACS. Platelet-derived MPs have been excessively studied, since they have been found to trigger the coagulation cascade; however, their role as predictors of future cardiovascular events remains debatable. The role of red blood cell-derived MPs is more intriguing; they have been proposed as markers of ongoing thrombosis in patients with ACS, while previous studies have shown that they have anti-coagulant properties in healthy individuals. Leukocyte-derived MPs may also have a predictive role, although the studies regarding these are still limited. Last but not least, it was an interesting discovery that circulating MPs can provide information regarding the angiographic lesions in patients with ACS.Conclusion:The concept of MPs as potential circulating biomarkers in patients with ACS holds much promise. However, large-scale clinical studies are required to evaluate whether the measurement of plasma MPs could be of clinical significance and, thus, dictate a more aggressive treatment strategy in patients with high levels of circulating MPs.
Frequent Mobile Electronic Medical Records Users Respond More Quickly to Emergency Department Consultation Requests: Retrospective Quantitative Study
Authors: Jung Kwang Yul , Kim SuJin , Kim Kihyung , Lee Eun Ju , Kim Kyunga , Lee Jeanhyoung , Choi Jong Soo , Kang Mira , Chang Dong Kyung , Cha Won Chul 
Published: December 2019

Background
Specialty consultation is a critical aspect of emergency department (ED) practice, and a delay in providing consultation might have a significant clinical effect and worsen ED overcrowding. Although mobile electronic medical records (EMR) are being increasingly used and are known to improve the workflow of health care providers, limited studies have evaluated their effectiveness in real-life clinical scenarios.

Objective
For this study, we aimed to determine the association between response duration to an ED specialty consultation request and the frequency of mobile EMR use.

Methods
This retrospective study was conducted in an academic ED in Seoul, South Korea. We analyzed EMR and mobile EMR data from May 2018 to December 2018. Timestamps of ED consultation requests were retrieved from a PC-based EMR, and the response interval was calculated. Doctors’ log frequencies were obtained from the mobile EMR, and we merged data using doctors’ deidentification numbers. Pearson’s product-moment correlation was performed to identify this association. The primary outcome was the relationship between the frequency of mobile EMR usage and the time interval from ED request to consultation completion by specialty doctors. The secondary outcome was the relationship between the frequency of specialty doctors’ mobile EMR usage and the response time to consultation requests.

Results
A total of 25,454 consultations requests were made for 15,555 patients, and 252 specialty doctors provided ED specialty consultations. Of the 742 doctors who used the mobile EMR, 208 doctors used it for the specialty consultation process. After excluding the cases lacking essential information, 21,885 consultations with 208 doctors were included for analysis. According to the mobile EMR usage pattern, the average usage frequency of all users was 13.3 logs/day, and the average duration of the completion of the specialty consultation was 51.7 minutes. There was a significant inverse relationship between the frequency of mobile EMR usage and time interval from ED request to consultation completion by specialty doctors (coefficient=–0.19; 95% CI –0.32 to –0.06; P=.005). Secondary analysis with the response time was done. There was also a significant inverse relationship between the frequency of specialty doctors’ mobile EMR usage and the response time to consultation requests (coefficient=–0.18; 95% CI –0.30 to –0.04; P=.009).

Conclusions
Our findings suggest that frequent mobile EMR usage is associated with quicker response time to ED consultation requests.