bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of oncolytic ECHO-7 virus strain Rigvir on uveal melanoma cell lines
Authors: Tilgase Andra , Grīne Lita , Blāķe Ilze , Borodušķis Mārtiņs , Rasa Agnija , Alberts Pēteris 
Published: April 2020

Abstract
Objective
Uveal melanoma is a rare intraocular malignancy. Half of the patients diagnosed will develop metastases within 10 to 30 years, most commonly in the liver. Although there has been a significant development in the treatment of melanoma, no effective treatment to prevent or treat metastases of uveal melanoma is available. Oncolytic viruses are now being studied for various types of cancers and show promising results. Preclinical results show cytolytic activity of enteric cytopathic human orphan virus type 7 (ECHO-7) strain Rigvir in human melanoma, rhabdomyosarcoma, gastric adenocarcinoma, lung carcinoma and pancreas adenocarcinoma cell lines. The aim of this study was to test the possible cytolytic activity in human uveal melanoma cell lines.

Results
The results suggest cytolytic activity of oncolytic ECHO-7 virus strain Rigvir in MP41, 92-1 and Mel-202 cell lines.
An adolescent girl with signs and symptoms of anaphylaxis and negative immunologic workup: a case report
Authors: Green Sarah , Hicks Allison , Hilsendager Chelsea , Bauer Maureen , Frank Guido K. W. 
Published: April 2020

Abstract
Background
The increasing incidence of allergies and allergic reactions among children and adults has become a major public health concern. The etiology of allergic reactions can often be confirmed based on a detailed history and supportive testing. However, there are cases where the underlying factors are more complex and difficult to identify.

Case presentation
Here we present the case report of a 14-year-old Caucasian girl with weight loss and a 3-year history of reported angioedema culminating in five intensive care unit admissions over the course of 2.5 months. Her initial clinical presentation included hypotension, dyspnea, and reported facial edema, but allergy and immunological workup were negative. A psychiatric workup identified an eating disorder with food restriction, comorbid major depression, obsessive compulsive disorder, and posttraumatic stress disorder. A close collaboration between Adolescent Medicine, Allergy and Immunology, and Psychiatry helped disentangle medical from psychiatric problems, including fluoxetine medication effects, as well as develop a treatment plan that was acceptable to the family. The angioedema was ultimately diagnosed as factitious disorder.

Conclusions
This patient’s treatment highlights the importance of a multidisciplinary team approach, a multifactorial etiology that needed to be addressed by multiple specialists, and the importance of long-term treatment and support.
DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration
Authors: Xiong Liu-Lin , Qiu De-Lu , Xiu Guang-Hui , Al-Hawwas Mohammed , Jiang Ya , Wang You-Cui , Hu Yue , Chen Li , Xia Qing-Jie , Wang Ting-Hua 
Published: April 2020

Abstract
Background
The limited neuronal differentiation of the endogenous or grafted neural stem cells (NSCs) after brain injury hampers the clinic usage of NSCs. Panax notoginseng saponins (PNS) were extensively used for their clinical value, such as in controlling blood pressure, blood glucose, and inhibiting neuronal apoptosis and enhancing neuronal protection, but whether or not it exerts an effect in promoting neuronal differentiation of the endogenous NSCs is completely unclear and the potential underlying mechanism requires further exploration.

Methods
Firstly, we determined whether PNS could successfully induce NSCs to differentiate to neurons under the serum condition. Mass spectrometry and quantitative polymerase chain reaction (Q-PCR) were then performed to screen the differentially expressed proteins (genes) between the PNS + serum and serum control group, upon which dihydropyrimidinase-like 2 (DPYSL2), a possible candidate, was then selected for the subsequent research. To further investigate the actual role of DPYSL2 in the NSC differentiation, DPYSL2-expressing lentivirus was employed to obtain DPYSL2 overexpression in NSCs. DPYSL2-knockout rats were constructed to study its effects on hippocampal neural stem cells. Immunofluorescent staining was performed to identify the differentiation direction of NSCs after 7 days from DPYSL2 transfection, as well as those from DPYSL2-knockout rats.

Results
Seven differentially expressed protein spots were detected by PD Quest, and DPYSL2 was found as one of the key factors of NSC differentiation in a PNS-treated condition. The results of immunostaining further showed that mainly Tuj1 and GFAP-positive cells increased in the DPYSL2-overexpressed group, while both were depressed in the hippocampal NSCs in the DPYSL2-knockout rat.

Conclusions
The present study revealed that the differentiation direction of NSCs could be enhanced through PNS administration, and the DPYSL2 is a key regulator in promoting NSC differentiation. These results not only emphasized the effect of PNS but also indicated DPYSL2 could be a novel target to enhance the NSC differentiation in future clinical trials.
Capsulized faecal microbiota transplantation ameliorates post-weaning diarrhoea by modulating the gut microbiota in piglets
Authors: Tang Wenjie , Chen Daiwen , Yu Bing , He Jun , Huang Zhiqing , Zheng Ping , Mao Xiangbing , Luo Yuheng , Luo Junqiu , Wang Quyuan , Wang Huifen , Yu Jie 
Published: April 2020

AbstractEarly weaning-induced stress causes diarrhoea, thereby reducing the growth performance of piglets. Gut bacterial dysbiosis has emerged as a leading cause of post-weaning diarrhoea. The present study aimed to investigate the effect of capsulized faecal microbiota transplantation (FMT) on the gut bacterial community, immune response and gut barrier function of piglets. Thirty-two weaned barrows were randomly divided into two groups. The recipient group was inoculated orally with capsulized faecal microbiota of healthy Tibetan pigs during the whole period of the trial, while the control group was given an empty capsule. The feed-to-gain ratio, diarrhoea ratio, and histological damage score of recipient piglets were significantly decreased. FMT treatment significantly increased the colon length of piglets. Furthermore, the relative abundances ofFirmicutes,Euryarchaeota,Tenericutes,Lactobacillus, andMethanobrevibacterin the colon of recipient piglets were increased, and the relative abundances ofCampylobacterandProteobacteriawere significantly decreased compared with those in the control group. CD4+lymphocytes and CD4+/CD8+ratio in the peripheral blood of recipient piglets were significantly increased. FMT treatment increased the IL-4 and IL-10 levels and decreased the TNF-α and INF-γ levels in the colonic tissue of piglets. The recipient piglets’ mRNA expression of TLR2, TLR8, NF-κB, and iNOS was significantly regulated. In addition, FMT significantly enhanced the gene expression of ZO-1. Overall, treatment with capsulized FMT ameliorated diarrhoea in piglets, with significant effects on limiting colon inflammatory responses, downregulating the TLR signalling pathway and the gene expression of iNOS, and strengthening intestinal barrier function by modulating the constituents of the gut microbiota.
Solutions of boundary value problems on extended-Branciari b-distance
Authors: Abdeljawad Thabet , Karapınar Erdal , Panda Sumati Kumari , Mlaiki Nabil 
Published: April 2020

AbstractIn this paper, we consider a new distance structure, extended Branciarib-distance, to combine and unify several distance notions and obtain fixed point results that cover several existing ones in the corresponding literature. As an application of our obtained result, we present a solution for a fourth-order differential equation boundary value problem.
Some oscillation theorems for nonlinear second-order differential equations with an advanced argument
Authors: Chatzarakis G. E. , Moaaz O. , Li T. , Qaraad B. 
Published: April 2020

AbstractThe objective in this work is to study oscillation criteria for second-order quasi-linear differential equations with an advanced argument. We establish new oscillation criteria using both the comparison technique with first-order advanced differential inequalities and the Riccati transformation. The established criteria improve, simplify and complement results that have been published recently in the literature. We illustrate the results by an example.
Attachment style and addictions (alcohol, cigarette, waterpipe and internet) among Lebanese adolescents: a national study
Authors: Nakhoul Laurette , Obeid Sahar , Sacre Hala , Haddad Chadia , Soufia Michel , Hallit Rabih , Akel Marwan , Salameh Pascale , Hallit Souheil 
Published: April 2020

AbstractBackgroundThe prevalence in the Lebanese general population of cigarette and waterpipe smoking, alcohol drinking and internet use seems to be increasing lately. So far, no study was done relating the above to attachment styles in Lebanese adolescents. Consequently, the objective of our study was to assess the relationship between attachment styles (secure, preoccupied, fearful, and dismissing) and addictions (cigarettes, water pipes, alcohol, and internet) among this population.MethodsIt is a cross-sectional study that took place between January and May 2019. Two thousand questionnaires were distributed out of which 1810 (90.5%) were completed and collected back. A proportionate random sample of schools from all Lebanese Mohafazat was used as recruitment method.ResultsA secure attachment style was significantly associated with lower addiction to alcohol, cigarette, and waterpipe, whereas insecure attachment styles (preoccupied, dismissing and fearful) were significantly associated with higher addiction to cigarette, waterpipe, alcohol, and internet.ConclusionLebanese adolescents with insecure attachment had higher rates of addiction to cigarette, waterpipe, alcohol, and internet. They should be closely monitored in order to reduce the risk of future substance use disorder and/or behavioral addiction development.
Development of a small panel of SNPs to infer ancestry in Chileans that distinguishes Aymara and Mapuche components
Authors: Verdugo Ricardo A. , Di Genova Alex , Herrera Luisa , Moraga Mauricio , Acuña Mónica , Berríos Soledad , Llop Elena , Valenzuela Carlos Y. , Bustamante M. Leonor , Digman Dayhana , Symon Adriana , Asenjo Soledad , López Pamela , Blanco Alejandro , Suazo José , Barozet Emmanuelle , Caba Fresia , Villalón Marcelo , Alvarado Sergio , Cáceres Dante , Salgado Katherine , Portales Pilar , Moreno-Estrada Andrés , Gignoux Christopher R. , Sandoval Karla , Bustamante Carlos D. , Eng Celeste , Huntsman Scott , Burchard Esteban G. , Loira Nicolás , Maass Alejandro , Cifuentes Lucía 
Published: April 2020

Abstract
Background
Current South American populations trace their origins mainly to three continental ancestries, i.e. European, Amerindian and African. Individual variation in relative proportions of each of these ancestries may be confounded with socio-economic factors due to population stratification. Therefore, ancestry is a potential confounder variable that should be considered in epidemiologic studies and in public health plans. However, there are few studies that have assessed the ancestry of the current admixed Chilean population. This is partly due to the high cost of genome-scale technologies commonly used to estimate ancestry. In this study we have designed a small panel of SNPs to accurately assess ancestry in the largest sampling to date of the Chilean mestizo population (n = 3349) from eight cities. Our panel is also able to distinguish between the two main Amerindian components of Chileans: Aymara from the north and Mapuche from the south.

Results
A panel of 150 ancestry-informative markers (AIMs) of SNP type was selected to maximize ancestry informativeness and genome coverage. Of these, 147 were successfully genotyped by KASPar assays in 2843 samples, with an average missing rate of 0.012, and a 0.95 concordance with microarray data. The ancestries estimated with the panel of AIMs had relative high correlations (0.88 for European, 0.91 for Amerindian, 0.70 for Aymara, and 0.68 for Mapuche components) with those obtained with AXIOM LAT1 array. The country’s average ancestry was 0.53 ± 0.14 European, 0.04 ± 0.04 African, and 0.42 ± 0.14 Amerindian, disaggregated into 0.18 ± 0.15 Aymara and 0.25 ± 0.13 Mapuche. However, Mapuche ancestry was highest in the south (40.03%) and Aymara in the north (35.61%) as expected from the historical location of these ethnic groups. We make our results available through an online app and demonstrate how it can be used to adjust for ancestry when testing association between incidence of a disease and nongenetic risk factors.

Conclusions
We have conducted the most extensive sampling, across many different cities, of current Chilean population. Ancestry varied significantly by latitude and human development. The panel of AIMs is available to the community for estimating ancestry at low cost in Chileans and other populations with similar ancestry.
The feasibility and impact of instrument-assisted manual therapy (IAMT) for the lower back on the structural and functional properties of the lumbar area in female soccer players: a randomised, placebo-controlled pilot study design
Authors: Weber Patrick , Graf Christine , Klingler Werner , Weber Nadine , Schleip Robert 
Published: April 2020

Abstract
Background
Myofascial (self-)treatments, such as foam rollers to therapeutic instruments in manual therapy, are utilized increasingly in prevention and therapy in healthy people, athletes, and patients suffering from chronic back pain. However, there is limited knowledge about the effectiveness of treatment and the underlying mechanisms of myofascial therapies, especially for instrument-assisted manual therapy (IAMT). Therefore, this pilot study will investigate the feasibility and impact of IAMT for the lumbar area compared with heat application and placebo treatment as a basis for calculating the sample size for further full studies. The primary outcomes will be a critical analysis of the feasibility of the measurement protocol in terms of time economy and expressiveness and of the short- and long-term effects on shear motion of the single tissue layers of the lower back obtained through ultrasound imaging. Secondary outcomes will include thickness and compressibility of the lumbar structures and flexibility of the dorsal structures, indentometry, and superficial skin temperature.

Methods
A minimum of 60 healthy, competitive 15–35-year-old female soccer players will be recruited and randomised into three groups. Short-term effects of IAMT on thoracolumbar structures will be compared with heat application and pressure-less placebo treatment. Long-term effects in the IAMT group will be tested after nine further interventions over a 5-week period (2×/week) and compared with the placebo group, which will not receive further treatments but will serve as a control. Intermediate and final testing of both groups will occur in weeks three and five.

Discussion
This pilot study will assess the feasibility and the impact of IAMT for the lower back particularly by examining the structural and functional properties of myofascial tissue using diagnostic ultrasound. These outcomes could evaluate the feasibility of the measurements used, shall build a basis for sample size calculation of further full studies, and might generate a greater understanding of myofascial therapies, especially IAMT, for the lower back and its benefits. If this approach proves to be practicable, next steps will be further full studies with soccer players, other sports, and patients with low back pain.

Trial registration
German Clinical Trials Register (DRKS00012252) 20.06.2018; retrospectively registered.
Psychometric properties of Turkish version of Jenkins sleep scale in fibromyalgia syndrome
Authors: Unal-Ulutatar Cagri , Ozsoy-Unubol Tugba 
Published: April 2020

AbstractBackgroundFibromyalgia syndrome (FMS) has adverse effects on the quality of sleep. The aim of this study was to investigate the validity and reliability of Jenkins Sleep Scale (JSS-TR) in Turkish FMS patients.MethodsFMS patients who met the 2016 fibromyalgia diagnostic criteria were included in the study. Clinical and demographic data of the patients were noted. The relationship between this scale and other functional parameters such as Pittsburgh Sleep Quality Index (PSQI), European Quality of Life Scale-5 Dimensions (EQ-5D), Fatigue Severity Scale (FSS), Beck Depression Inventory (BDI) was examined. Fibromyalgia Impact Questionnaire (FIQ) was used to evaluate the functional status of the patients and the progression of the disease. Test-retest reliability was calculated by re-applying the questionnaire to patients at 2-week intervals. Duloxetine treatment was initiated in newly diagnosed patients and sensitivity to change was tested at the end of the treatment. Spearman correlation coefficient was used.P < 0.05 was accepted as significant.ResultsEighty-one FMS patients (71 females, 10 males) were included in the study. The mean age was 44.2 ± 10.7 years. The strongest correlation of JSS-TR was with another sleep questionnaire, PSQI (rho = 0.79,p < 0.0005). The correlation with other functional parameters and FIQ was moderate. In test-retest validity, intraclass correlation coefficient was found to be 0.98 (p < 0.0005). Chronbach α value calculated for internal consistency was found to be 0.741.ConclusionsJSS-TR is a valid, simple and feasible sleep instrument that can be easily applied to FMS patients both in researches and clinical settings.
Influence of microwave on chromium complex composition in tanning liquor
Authors: Zhang Jinwei , Chen Wuyong , Gaidau Carmen 
Published: April 2020

Abstract
Since microwave irradiation could promote hydrolysis and olation of chromium tanning liquor, but the influence of microwave on chromium complex component in the liquor was still unknown. Chromium sulphate solution (0% basicity) and 33% basicity chromium tanning liquor were subjected microwave (MW) and water bath (WB) heating, and the samples without any warming were regarded as control. Ion exchange chromatography (IEC) and gel filtration chromatography (GFC) were used to measure the charge composition and molecular size of chromium complexes in each sample. FT-IR was used to characterize the structure of chromium complexes in each composition separated by IEC. Moreover, the chromium tanning liquor after warming was used in hide powder tanning trials to illustrate whether microwave would affect its tanning ability. The results show there are more high positive charge and large molecular size complexes in chromium tanning liquor after warming but the phenomena are more significant in MW samples compared with WB due to non-thermal effect of microwave. In addition, microwave has more powerful effect on 33% basicity chromium tanning liquor hydrolysis and olation to generate larger molecular size complexes. In FT-IR results, the combination pattern between chromium and ligands are changed after warming but there is no difference between WB and MW. The chromium exhaustion and thermal stability of hide powder tanned with chromium tanning liquor after microwave irradiation are both higher. It could conclude that both thermal and non-thermal effects of microwave promote the process together, and the non-thermal effect leads to more high positive charge and large molecular size complexes and has stronger influence on high polarity system. In short, this work would provide theoretical basis for applying microwave in tanning agent modification and chrome tanning process further.

Graphical abstract
Late-onset personality disorder: a condition still steeped in ignorance
Authors: Tyrer Peter , Howard Robert 
Published: April 2020

SUMMARYPersonality disorder is likely to be common in late life, but our ignorance is such that, at present, we can only speculate about its frequency and importance. The only firm evidence we have is that antisocial personality features tend to be attenuated in older age and obsessional and detached features accentuated. Differentiating personality change following organic disease from personality disorder requires more attention as it is important for good clinical management.
Comparison of Treatment, Cost, and Survival in Patients With Metastatic Colorectal Cancer in Western Washington, United States, and British Columbia, Canada
Authors: Yezefski Todd A. , Le Dan , Chen Leo , Speers Caroline H. , Chennupati Shasank , Snider Jeremy , Gill Sharlene , Ramsey Scott D. , Kennecke Hagen F. , Shankaran Veena 
Published: April 2020

PURPOSE: Few studies have directly compared health care utilization, costs, and outcomes between patients treated in the US multipayer health system and Canada’s single-payer system. Using cancer registry and claims data, we assessed treatment types, costs, and survival for patients with metastatic colorectal cancer (mCRC) in Western Washington State (WW) and British Columbia (BC). MATERIALS AND METHODS: Patients age ≥ 18 years diagnosed with mCRC in 2010 and later were identified from the BC Cancer database and a regional database linking WW SEER to claims from Medicare and two large commercial insurers. Demographics, treatment characteristics, costs of systemic therapy, and survival data were obtained from these databases and compared between the two regions. RESULTS: A total of 1,592 patients from BC and 901 from WW were included in the study. Median age was similar (BC, 66 years; WW, 63 years), but patients in BC were more likely to be male (57.1% v 51.2%; P ≤ .01) and to have de novo metastatic disease (61.0% v 38.3%; P ≤ .01). The use of radiation therapy was similar between regions (BC, 31.2%; WW, 33.9%; P = .18), but primary tumor resection was more common in BC (74.1% v 66.3%; P ≤ .01) as was hepatic metastasectomy (12.4% v 2.3%; P ≤ .01). Similar percentages of patients received systemic therapy (BC, 68.8%; WW, 67.1%; P = .40), but costs were significantly higher for first-line systemic therapy in WW ($6,226 v $15,792 per patient per month; P ≤ .01). Median overall survival was similar (BC, 16.9 months; WW, 18 months). CONCLUSION: Cost of systemic therapy for mCRC was significantly higher for patients in WW than in BC, but this did not translate to a difference in overall survival.