bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic relevance of Ki-67 and P16/INK4a markers in histological diagnosis of cervical dysplasia
Authors: Dimitriadi T. A. , Burtsev D. V. , Dzhenkova E. A. , Gudtskova T. N. , Dvadnenko K. V. 
Published: March 2020

Purpose of the study. To determine the quantitative parameters of the molecular markers Ki‑67 and p16/INK4a at CIN 1–3 and to establish the possibility of using them to improve the quality of diagnosis by histological samples.Patients and methods. Biopsy material of the cervix was studied in 71 patients who were carriers of HPV infection. Immunohistochemical method (IHC) in biopsy specimens determined the expression of Ki‑67 and p16/INK4a markers.Results. According to the results of a standard histological examination, the distribution of patientsinto groups occurred as follows: CIN 1–18, in CIN 2–39, in CIN 3–14 patients. It was found that the expression of the studied markers is associated with the severity of dysplastic changes in the tissue of the cervix. By the studied marker, the most characteristic molecular profiles for the degrees of dysplasia were determined: CIN1 – Ki‑67–15–25%, p16/INK4a 10–15%; CIN2 – Ki‑67–70–80%, p16/INK4a – 65–70%; CIN3 – Ki‑67–85–90%, p16/INK4a – 90–95%. Heterogeneity was revealed in the expression of these markersin the CIN 2 group: in 7 cases (22,6%), the molecular profile corresponded to CIN 1, in 1 case (3,6%) to CIN 3. Clinical examples of using the IHC profile to clarify the degree of CIN are given.Conclusion. The use of an IHC study with Ki‑67 and p16/INK4a, in addition to the standard histological examination, makes it possible to objectify the initial diagnosis of the degree of CIN, as well as to identify patients with a high and low risk of developing severe injuries. This is especially true for the CIN2 group, the most problematic in terms of histological evaluation and tactics of clinical management of patients. The use of IHC in the initial diagnosis will contribute to: improving the information content of multiple aimed and loop excisional biopsies; reduction in relapse due to inadequate treatment; eliminating the unreasonable use of such a traumatic method as conization (especially in women of childbearing age).
Çiğ ve UHT Sütlerinde Kimyasal Parametre Değişimlerinin Tespiti
Authors: SUCAK Mustafa Güçlü , ÖZKAYA Ahmet , AĞYAR Oğuz , YILMAZ Eray 
Published: March 2020

Bu çalışmada, çiğ ve ticari UHT inek sütlerinin biyokimyasal parametreleri yönünden karşılaştırılması amaçlandı. Bu amaçla, süt numunelerinin mineral ve kimyasal bileşen parametre seviyeleri, sırasıyla İndüklenmiş Eşleşmiş Plazma Atomik Emisyon Spektrofotometresi ve Foss Electrik süt analizörü cihazları kullanılarak ölçüldü. Doğal inek çiğ sütleri Muş Bölgesinden temin edilirken, ticari amaçla üretilen UHT sütleri Adıyaman ilindeki yerel marketlerden temin edildi. UHT sütleri tam yağlı ve yarım yağlı olarak iki gruba ayrıldı. Çiğ süt minerallerinden kalsiyum, demir, mangan ve fosfor düzeylerinin ticari sütlere göre daha yüksek çıktığı belirlendi. Ayrıca, çiğ süt kimyasal parametrelerinden yağsız kuru madde, yağ ve protein düzeylerinin de daha yüksek çıktığı gözlendi. Sonuçlarımıza göre ticari inek sütlerin besinsel düzeylerindeki azalmanın insan sağlığını etkileyebilecek oranda olmadığını düşünmekteyiz.
Veteriner İlaçlarının Çevrede Bulunuşu ve Etkileri
Authors: ÖZGÜVEN AYŞE 
Published: March 2020

Veteriner
ilaçları hastalığı tedavi etmek ve hayvan sağılığını korumak için yaygın olarak
kullanılmaktadır. Hayvan hastalıklarının tedavisinde ve önlenmesinde kullanılan
veteriner ilaçları, yoğun tarımsal ve su ürünleri yetiştiriciliğinden dolayı
çevre kirliliğinin önemli bir kaynağını oluşturmaktadır. Hayvancılık ve
tavukçuluk üretiminde kullanılan farmasötik maddeler hayvanlar tarafından
metabolize edildikten sonra dışkı yoluyla çevresel ortamlara bırakılırlar. Hayvan yemi ve kullanılmayan ilaçların uygunsuz
bertarafı sonucunda yüzeysel su kaynaklarına kadar ulaşabilirler. Ayrıca hayvan
gübrelerinin tarımsal alanlarda kullanılması veteriner ilaçlarının çevrede
bulunmasına neden olmaktadır. Bu derlemede veteriner uygulamalarında yaygın
olarak kullanılan ilaçların çevresel ortamlarda bulunuşu, davranışı,
dönüşümü ve canlılar üzerindeki toksik
etkileri hakkında mevcut bilgiler verilmektedir. Ayrıca düşük konsantrasyondaki ilaçların ve
metabolitlerinin uzun vadeli etkileri, ekosistemdeki veteriner ilaçlarının davranışı
ve çevreye etkileri araştırılmıştır.
Karadeniz Teknik Üniversitesi Kanuni Kampüsü’nde Bazı Ofislerde Radon Gazı Ölçümü ve Çevrelerindeki Topraklarda Radyonüklid Seviyeleri
Authors: UZUN DURAN Selcen , KÜÇÜKÖMEROĞLU Belgin 
Published: March 2020

Bu
çalışmada KTÜ Kanuni Kampüsünde ofislerde radon gazı seviyesi ölçülmüş ve
çalışanlar için radondan kaynaklanan doz eşdeğerleri tahmin edilmiştir. Radon
gazı ölçümleri 30 ofiste yapılmıştır.
Ölçümler üçer katlı on binanın her bir katında yapılmış ve katlara göre
radon gazı değerleri karşılaştırılmıştır. Çalışanlar zamanının çoğunu
ofislerinde geçirdikleri için ofislerde radon gazı ölçümü önemlidir. Radon gazı
ölçümleri ALPHAGUARD PQ 2000 radon monitörü ile ölçülmüş sonuçlar uluslararası
değerler ile karşılaştırılmıştır. KTÜ Kanuni Kampüsünde ölçülen radon gazı
değerleri izin verilen limit değerlerin altındadır. Ayrıca seçilen bu 10
binanın etrafından alınan toprak örneklerinde radyonüklid analizi yapılmış ve
değerler uluslararası kuruluşların izin verdiği limit seviyelerle
karşılaştırılmıştır.
MICROCLIMA Y CONFORT TËRMICO
Authors: Therán Nieto Kevin Rafael 
Published: March 2020

El presente artículo presenta una revisión de la literatura existente en cuanto al diseño de espacios urbanos, microclima y confort térmico urbano. El microclima y confort térmico en espacios urbanos, especialmente en las ciudades localizadas en regiones tropicales, debe ser abordada a partir de los conceptos y teorías que permiten comprender la incidencia de los parámetros microclimáticos y tejido urbano sobre la sensación de confort térmico y bioclimatización de los espacios urbanos. De esta forma, en este documento se manifiesta la significación de la planificación, el diseño urbano y microclima de una ciudad en regiones tropicales, explorando la clasificación de las zonas climáticas locales a nivel urbano, la isla de calor urbana, el confort térmico urbano, los parámetros microclimáticos como temperatura del aire, humedad relativa, viento, radiación solar e iluminación natural, la vegetación y su caracterización en espacios urbanos y la morfología de una ciudad.
AN INVESTIGATION ON THE EFFECT OF AUDIT COMMITTEE ON FINANCIAL REPORTING QUALITY IN PRE AND POST IFRS ADOPTION: EVIDENCE FROM MALAYSIAN COMPANIES
Authors: Jatiningrum Citrawati , Fauzi  , Irviani Rita , Mujiyati  , Hasan Shahanif 
Published: March 2020

Purpose of study: This study sought to investigate the effect of the audit committee on Financial Reporting Quality (FRQ), explicitly focuses on the period pre- and post-mandatory IFRS adoption in Malaysia. The Financial Reporting Quality in this study proxied by earnings management. Malaysian.
Methodology: The sample study has covered 81 listed companies on Bursa Malaysia, with 567 observations, which examined the time of 2009 to 2015. The relationship was analyzed by statistical multiple regression linear methods and also examined the significance of differences between pre and post IFRS adoption by paired sample t-test.
Result: The main finding reveals that the relationship between the audit committee and financial reporting quality after IFRS adoption in Malaysia has more significant. However, empirical evidence showed that the post period of mandatory IFRS evidently no significant difference level of earnings management practice. This result indicates that the IFRS adoption cannot reduce managerial discretion yet and the possibility for EM manipulation for Malaysian companies.
Implication/Application: This finding has critical implications for regulators and policymakers, that the consequences of IFRS adoption do not increase the quality of financial reporting when EM practices still continue in the different forms.
Novelty/Originality of this study: This study gives empirical evidence that there are differences in relationship level between audit quality and earnings management in the period before and after IFRS mandatory adoption in Malaysia companies.
A NYELVI JOGSÉRTÉSEK SZANKCIONÁLHATÓSÁGÁNAK TÉTELES JOGI ÉS NEMZETKÖZI JOGI DIMENZIÓI
Authors: BERETKA Katinka 
Published: March 2020

A tanulmány a jogtudomány módszereivel mutatja be a szerbiai nyelvi jogi szabályozást és az ezen szabályok megsértésének szankcióit, illetve egyéb negatív jogkövetkezményeit, különösképpen a nemzeti kisebbségek hivatalos nyelvhasználatának szemszögéből, mind a tételes jog, mind pedig a nemzetközi jog által lefektetett keretek között. A sokat és egyes elemeiben méltán dicsért szerbiai nyelvi jogok nagy vonalakban történő ismertetése mellett a hangsúly az esetleges büntető rendelkezések, illetve azok hatékonyságának kritikai analízisén van, figyelemmel arra a nem éppen kedvező gyakorlatra Szerbiában, hogy sok esetben a jogsértő magatartás semmilyen következményt nem von maga után a jogszegőre nézve.
A cél összegyűjteni azon jogszabályhelyeket, amelyek valamilyen szankciót, felelősségre vonást írnak elő a nyelvi jogsértések esetére, és lehetőség szerint értékelni azok eredményességét a nemzeti kisebbségek nyelvi jogérvényesítésének folyamatában.
Self-Analysis of Repeat Proteins Reveals Evolutionarily Conserved Patterns
Authors: Merski Matthew , Młynarczyk Krzysztof , Ludwiczak Jan , Skrzeczkowski Jakub , Dunin-Horkawicz Stanisław , Górna Maria W. 
Published: March 2020

Abstract
Background

Protein repeats can confound sequence analyses because the repetitiveness of their amino acid sequences lead to difficulties in identifying whether similar repeats are due to convergent or divergent evolution. We noted that the patterns derived from traditional “dot plot” protein sequence self-similarity analysis tended to be conserved in sets of related repeat proteins and this conservation could be quantitated using a Jaccard metric.

Results

Comparison of these dot plots obviated the issues due to sequence similarity for analysis of repeat proteins. A high Jaccard similarity score was suggestive of a conserved relationship between closely related repeat proteins. The dot plot patterns decayed quickly in the absence of selective pressure with an expected loss of 50% of Jaccard similarity due to a loss of 8.2 % sequence identity. To perform method testing, we assembled a standard set of 79 repeat proteins representing all the subgroups in RepeatsDB. Comparison of known repeat and non-repeat proteins from the PDB suggested that the information content in dot plots could be used to identify repeat proteins from pure sequence with no requirement for structural information. Analysis of the UniRef90 database suggested that 16.9% of all known proteins could be classified as repeat proteins. These 13.3 million putative repeat protein chains were clustered and a significant amount (82.9%) of clusters containing between 5 and 200 members were of a single functional type.

Conclusions

Dot plot analysis of repeat proteins attempts to obviate issues that arise due to the sequence degeneracy of repeat proteins. These results show that this kind of analysis can efficiently be applied to analyze repeat proteins on a large scale.
The loss of photosynthesis pathway and genomic locations of the lost plastid genes in a holoparasitic plant Aeginetia indica
Authors: Chen Jingfang , Yu Runxian , Dai Jinhong , Liu Ying , Zhou Renchao 
Published: March 2020

Abstract
Background: With three origins of holoparasitism, Orobanchaceae provides an ideal system to study the evolution of holoparasitic lifestyle in plants. The evolution of holoparasitism can be revealed by plastid genome degradation and coordinated changes in the nuclear genome, since holoparasitic plants lost the capability of photosynthesis. Among the three clades with holoparasitic plants in Orobanchaceae, only Clade VI has no available plastid genome sequences for holoparasitic plants. In this study, we sequenced the plastome and transcriptome of Aeginetia indica, a holoparasitic plant in Clade VI of Orobanchaceae, to study its plastome evolution and the corresponding changes in the nuclear genome as a response of the loss of photosynthetic function. Results: The plastome of A. indica is reduced to 86,212 bp in size, and almost all photosynthesis-related genes were lost. Massive fragments of the lost plastid genes were transferred into the mitochondrial or nuclear genomes. These fragments could not be detected in its transcriptomes, suggesting that they were non-functional. Most protein coding genes in the plastome showed the signal of relaxation of purifying selection. Plastome and transcriptome analyses indicated that the photosynthesis pathway is completely lost, and that the porphyrin and chlorophyll metabolism pathways are partially retained, although chlorophyll synthesis is not possible.Conclusions: Our study suggests the loss of photosynthesis-related functions in A. indica in both the nuclear and plastid genomes. The lost plastid genes are transferred into its nuclear and mitochondrial genomes, and exist in very small fragments with no expression and are thus non-functional. The Aeginetia indica plastome also provides a resource for comparative studies on the repeated evolution of holoparasitism in Orobanchaceae.
Impact of dietary Mannan-oligosaccharide and β-Glucan supplementation on growth, histopathology, E-coli colonization and hepatic transcripts of TNF-α and NF- ϰB of broiler challenged with E. coli O78
Authors: Fadl Sabreen Ezzat , El-Gammal Ghada Ahmed , Sakr Osama Atia , Salah Aly A.B.S. , Atia Ayman Ali , Prince Abdelbary Mohammed , Hegazy Abdelhaleem Mohamed 
Published: March 2020

Abstract
Background: Using probiotics have become popular. They are considered an alternative to Antibiotic Growth Promoters (AGP). Probiotics are supplemented into animal feed for improving growth performance along with preventing and controlling enteric pathogens. The aim of this work was to study the impact of dietary supplementation of Mannan-oligosaccharide and β-Glucan (Agrimos®) on broiler challenged with Escherichia coli O78 (E. coli O78 - marked with an antibiotic (320 μg ciprofloxacin/ml broth) on growth performance, serum biochemistry, immune organs-histopathology, E-coli colonization, and hepatic transcripts of Tumor necrosis factor-alpha (TNF-α) and Nuclear factor-kappa B (NF-ϰB).Methods: A total of 125 one-day-old chicks were used for conducting the experiment. Five one-day-old chicks were slaughtered for measuring the initial weight of lymphoid tissue. The remaining chicks (120) were allotted into four groups according to Mannan-oligosaccharide and β-Glucan supplementation and E. coli infection. The data were analyzed using SPSS version 16.Results: Results indicated significant alteration of growth performance, serum biochemistry, selected liver gene expression with pathological lesions especially in lymphoid organs due to E. coli infection. These alterations were mitigated by Mannan-oligosaccharide and β-Glucan supplementation.Conclusion: It could be concluded Mannan-oligosaccharide and β-Glucan supplementation in broiler's diet improved the immune response of broilers and mitigated pathological lesion resulted from E. coli infection.
NMNAT3 improves mitochondrial function and enhances BMSCs anti-oxidative stress through the NAD+-Sirt3 pathway
Authors: Wang Tao , Peng Wuxun , Zhang Fei , Wang Lei , Zhang Jian , Dong Wentao , Ye Chuan , Tian Xiaobin , Li Yanlin , Gong Yuekun 
Published: March 2020

Abstract
Background To investigate the effects of NMNAT3 on mitochondrial function and anti-oxidative stress in rabbit BMSCs and its underlying mechanisms. Methods Stable strains of NMNAT3 overexpressing rabbit BMSCs were obtained by lentivirus transfection; the Oxidative stress model in rabbit BMSCs was imitated by treating with H 2 O 2 ; Observe the changes in mitochondrial ultrastructure and mitochondrial function-related indicators (mitochondrial membrane potential, ATP and mitochondrial protein PGC-1α, NRF1 synthesis), to study the effect of NMNAT3 on improving mitochondrial function under oxidative stress; detect ROS and lipids Peroxidation products (MDA content), antioxidant enzymes (CAT and GPx) activity, SA-β-Gal activity and apoptosis in rabbit BMSCs, to study the changes of the anti-oxidative stress ability of BMSCs modified by NMNAT3;Then, the selective Sirt3 inhibitor (3-TYP) was used to analyze the mechanism of NMNAT3 improve mitochondrial function and antioxidant stress ability of BMSCs by regulating mitochondrial NAD + levels, and whether it affects the acetylation levels of mitochondrial target proteins (Idh2 and FOXO3a) and the expression and activation of effect proteins (GSH, ATP synthase and Mn-SOD) through Sirt3 pathway. Results Overexpression of NMNAT3 can improve the mitochondrial ultrastructural damage of rabbit BMSCs under oxidative stress; increase the mitochondrial membrane potential, increase the synthesis of ATP and mitochondrial protein PGC-1α and NRF1,significantly improve the function of mitochondria.It also can significantly enhance the antioxidant stress ability of rabbit BMSCs by reducing ROS level and MDA content, increasing the activities of antioxidant enzymes CAT and GPx, and decreasing cell senescence and apoptosis under stress.Rabbit BMSCs overexpressed by NMNAT3 significantly increased the activity of Sirt3, significantly decreased the acetylation levels of Idh2 and FOXO3a under oxidative stress, and increased the level of GSH and the activities of ATP synthase and Mn-SOD.However, the addition of 3-TYP basically blocked the protective effect of NMNAT3, indicating that Sirt3 is an important pathway for NMNAT3 to regulate mitochondrial function and antioxidant stress in BMSCs under oxidative stress. Conclusion NMNAT3 can effectively improve the mitochondrial function of rabbit BMSCs under oxidative stress through NAD + -Sirt3 pathway, and play a role in enhancing its antioxidant stress injury.
Down-regulated FOXO1 in refractory/relapse childhood B-cell acute lymphoblastic leukemia
Authors: ZHANG HUI , Jiang Chuang , Liu Haiyan , Hao Wenge , Wang Pengfei , Qian Maoxiang , Huang Haiying , Li Ziping , Qian Jiabi , Zheng Qingqing 
Published: March 2020

Abstract
Background Refractory/relapsed acute lymphoblastic leukemia (RR-ALL) remains to be a leading cause of treatment failure and subsequent death. Forkhead box O1 (FOXO1) belongs to the forkhead family of transcription factors, its specific role in RR-ALL has not yet been determined in B-cell ALL (B-ALL). The purpose of this study was to investigate the expression and prognostic value in ALL. Methods RNA sequencing was applied to an ALL case with induction failure to identify the causal events. The transcription activity was examined with luciferase reporter assay. FOXO1 mRNA expression level was examined using real-time quantitative PCR. Association analysis was performed to correlate FOXO1 transcription with childhood B-ALL prognosis and relapse. Results In this ALL case with induction failure, we successfully identified a novel MEIS1-FOXO1 fusion gene. The transcription activity of MEIS1-FOXO1 was significantly abolished as compared to wild-type FOXO1. Low FOXO1 expression level was strongly associate with unfavorable subtype, MRD positivity and relapse. Conclusions FOXO1 loss of function associates with ALL high-risk stratification and relapse, which might be due to drug resistance.
Thinking about the misdiagnosis: A case report of intra-abdominal desmoid-type fibromatosis and literature review
Authors: He XuChang , An Wenhui , Zeng Qingbin , Long Kui , Dong Wenzhi , Mo Xiaohua 
Published: March 2020

Abstract
Background Desmoid-type fibromatosis is a low grade soft tissue sarcoma that derives from mesenchymal progenitor cells. Without characteristics of imaging, desmoid-type fibromatosis often confused with stromal tumors clinically. The disease characterized by local invasiveness and local recurrence but rare do metastasize. It can occur anywhere in the body. Most common location is the abdominal wall, nearly 30%~50% in all cases. It can also occur in the abdominal cavity, with a low incidence. At present, all types of desmoid-type fibromatosis follow the treatment consensus of extra-abdominal type. However, because of the difficulty in preoperative diagnosis, sometimes it is hard to choose the appropriate treatment. Case presentation We describe the case of a 22-years-old woman, who was diagnosed with an abdominal mass . She was a healthy individual who was incidentally diagnosed with abdominal mass. The mass was under the suspicion of gastrointestinal stromal tumors in a local hospital, according to the result of MRI scan. A CT scan of her abdomen showed a huge hypoechoic mass in the hepatic. Under misdiagnosis as hemangioma of liver in our hospital, she underwent a laparoscopic resection of the tumor . Postoperatively, the tumor was confirmed to be a desmoid-type fibromatosis by patholgical and immunohistochemical findings. Conclusions In conclusion, due to the high risk of recurrence, the diagnosis before treatment is the most important part in the management of desmoid-type fibromatosis. Even if it is rare, DF should also be included in the differential diagnosis. Treatment needs to be evaluated according to the location of the lesion. For lesions on the body surface, pathologic examination will be safer and more useful. Surgery is still one of the best choice for intra-abdominal desmoid-type fibromatosis patients. Meanwhile, in order to change the operation in time, the clinicals should keep a rigorous attitude in surgery, if preoperative diagnosis is difficult.
Temporal summation of repetitive mechanical stimulation in patients with painful temporomandibular joints and healthy controls
Authors: Xi Xiaojing , Zhou Weina , Zhang Jinglu , Yu Linfeng , Wang Kelun , Svensson Peter 
Published: March 2020

Abstract
Aim: This study was conducted to test segmental and extra-segmental temporal summation (TS) effects of repetitive mechanical stimulation in patients with painful temporomandibular joints (TMJ) and healthy individuals. Methods: Twenty patients with unilateral pain in the TMJ and 20 gender- and age-matched healthy controls were included. A modified Quantitative Sensory Testing (QST) protocol was performed including pressure pain thresholds (PPT), mechanical pain thresholds (MPT), and numerical rating scale (NRS) scores of TS effects of 10 repeated 0.5 kg, 1.0 kg pressure stimuli and pinprick stimuli were determined as wind-up ratios (WUR) on the glabrous skin of the dominant hand (extra-segmental) and bilaterally in the TMJ region. Kruskal-Wallis test was used for data analysis. Results: The PPT and MPT were significantly lower on painful TMJs compared to non-painful TMJs (P=0.001) and control TMJs (P=0.001). WUR of repetitive mechanical stimulation on painful TMJs was higher compared to control TMJs (P ≤ 0.012). Non-painful TMJs also had significantly higher WUR of pinprick stimuli compared to control TMJs (P=0.008). Except the WUR of repetitive mechanical stimulation (P=0.030) no significant differences were detected at the hand for any of the test parameters between the two groups (P ≥ 0.05). Conclusion: Patients with unilateral TMJ pain have segmentally, but not extra-segmentally, increased sensitivity to mechanical stimuli including facilitated temporal summation mechanisms at segmental as well at extra-segmental sites.