bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of hypoxia and energy depletion on the response of endothelial cells to the vascular disrupting agent combretastatin A-4-phosphate
Authors: Holmes Toby , Brown Andrew W. , Suggitt Marie , Shaw Lucy A. , Simpson Lucy , Harrity Joseph P. A. , Tozer Gillian M. , Kanthou Chryso 
Published: June 2020

AbstractCombretastatin A-4 phosphate (CA4P) is a microtubule-disrupting tumour-selective vascular disrupting agent (VDA). CA4P activates the actin-regulating RhoA-GTPase/ ROCK pathway, which is required for full vascular disruption. While hypoxia renders tumours resistant to many conventional therapies, little is known about its influence on VDA activity. Here, we found that active RhoA and ROCK effector phospho-myosin light chain (pMLC) were downregulated in endothelial cells by severe hypoxia. CA4P failed to activate RhoA/ROCK/pMLC but its activity was restored upon reoxygenation. Hypoxia also inhibited CA4P-mediated actinomyosin contractility, VE-cadherin junction disruption and permeability rise. Glucose withdrawal downregulated pMLC, and coupled with hypoxia, reduced pMLC faster and more profoundly than hypoxia alone. Concurrent inhibition of glycolysis (2-deoxy-D-glucose, 2DG) and mitochondrial respiration (rotenone) caused profound actin filament loss, blocked RhoA/ROCK signalling and rendered microtubules CA4P-resistant. Withdrawal of the metabolism inhibitors restored the cytoskeleton and CA4P activity. The AMP-activated kinase AMPK was investigated as a potential mediator of pMLC downregulation. Pharmacological AMPK activators that generate AMP, unlike allosteric activators, downregulated pMLC but only when combined with 2DG and/or rotenone. Altogether, our results suggest that Rho/ROCK and actinomyosin contractility are regulated by AMP/ATP levels independently of AMPK, and point to hypoxia/energy depletion as potential modifiers of CA4P response.
Wolf habitat selection when sympatric or allopatric with brown bears in Scandinavia
Authors: Ordiz Andrés , Uzal Antonio , Milleret Cyril , Sanz-Pérez Ana , Zimmermann Barbara , Wikenros Camilla , Wabakken Petter , Kindberg Jonas , Swenson Jon E. , Sand Håkan 
Published: June 2020

AbstractHabitat selection of animals depends on factors such as food availability, landscape features, and intra- and interspecific interactions. Individuals can show several behavioral responses to reduce competition for habitat, yet the mechanisms that drive them are poorly understood. This is particularly true for large carnivores, whose fine-scale monitoring is logistically complex and expensive. In Scandinavia, the home-range establishment and kill rates of gray wolves (Canis lupus) are affected by the coexistence with brown bears (Ursus arctos). Here, we applied resource selection functions and a multivariate approach to compare wolf habitat selection within home ranges of wolves that were either sympatric or allopatric with bears. Wolves selected for lower altitudes in winter, particularly in the area where bears and wolves are sympatric, where altitude is generally higher than where they are allopatric. Wolves may follow the winter migration of their staple prey, moose (Alces alces), to lower altitudes. Otherwise, we did not find any effect of bear presence on wolf habitat selection, in contrast with our previous studies. Our new results indicate that the manifestation of a specific driver of habitat selection, namely interspecific competition, can vary at different spatial-temporal scales. This is important to understand the structure of ecological communities and the varying mechanisms underlying interspecific interactions.
Sex differences in the genetic architecture of depression
Authors: Kang Hee-Ju , Park Yoomi , Yoo Kyung-Hun , Kim Ki-Tae , Kim Eun-Song , Kim Ju-Wan , Kim Sung-Wan , Shin Il-Seon , Yoon Jin-Sang , Kim Ju Han , Kim Jae-Min 
Published: June 2020

AbstractThe prevalence and clinical characteristics of depressive disorders differ between women and men; however, the genetic contribution to sex differences in depressive disorders has not been elucidated. To evaluate sex-specific differences in the genetic architecture of depression, whole exome sequencing of samples from 1000 patients (70.7% female) with depressive disorder was conducted. Control data from healthy individuals with no psychiatric disorder (n = 72, 26.4% female) and East-Asian subpopulation 1000 Genome Project data (n = 207, 50.7% female) were included. The genetic variation between men and women was directly compared using both qualitative and quantitative research designs. Qualitative analysis identified five genetic markers potentially associated with increased risk of depressive disorder in females, including three variants (rs201432982 within PDE4A, and rs62640397 and rs79442975 within FDX1L) mapping to chromosome 19p13.2 and two novel variants (rs820182 and rs820148) within MYO15B at the chromosome 17p25.1 locus. Depressed patients homozygous for these variants showed more severe depressive symptoms and higher suicidality than those who were not homozygotes (i.e., heterozygotes and homozygotes for the non-associated allele). Quantitative analysis demonstrated that the genetic burden of protein-truncating and deleterious variants was higher in males than females, even after permutation testing. Our study provides novel genetic evidence that the higher prevalence of depressive disorders in women may be attributable to inherited variants.
Streptococcus agalactiae in childbearing age immigrant women in Comunitat Valenciana (Spain)
Authors: Sahuquillo-Arce José Miguel , Hernández-Cabezas Alicia , Castaño-Aroca María Jesús , Chouman-Arcas Rabab , Díaz-Aguirre Estefanía , Acosta-Boga Beatriz , López-Hontangas José Luis 
Published: June 2020

AbstractStreptococcus agalactiae (GBS) remains the leading cause of meningitis and neonatal sepsis in the world, and causes disease in pregnant and puerperal women. This is a retrospective study of GBS infections on women of childbearing age living in Comunitat Valenciana, Spain (years 2009–2014) and GBS colonization rate on pregnant women attending Hospital La Fe (years 2013–2015) according to their origin. An aggregated total of 6,641,960 women exposed during the study period had an average GBS isolation rate of 5.19‰ (5.14–5.25‰), geographical group rates being: Western Europe (2.2‰), North America (2.1‰), Australia (3.7‰), Spain (4.6‰), Latin America II (4.5‰), Eastern Europe (5.3‰), Asia (6.7‰), Latin America I (7.7‰), Middle East (7.9‰), Indian Subcontinent (17.2‰), North Africa (17.8‰), Sub-Saharan Africa (22.7‰). The 4532 pregnant women studied had an average GBS colonization rate of 12.47% (11.51–13.43) and geographical group rates varied similar to geographical isolation rates. Low GDP and high temperatures of the birth country were associated with higher colonization rates. Thus, differences in GBS colonization depend on the country of origin; Africa and the Indian subcontinent presented the highest, while Western Europe and North America had the lowest. This variability portrays a geographical pattern influenced by temperature and GDP.
The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte
Authors: Walter J. , Monthoux C. , Fortes C. , Grossmann J. , Roschitzki B. , Meili T. , Riond B. , Hofmann-Lehmann R. , Naegeli H. , Bleul U. 
Published: June 2020

AbstractIn vitro maturation (IVM) of oocytes has still a negative impact on the developmental competence of oocytes. Therefore, this study analysed the cumulus proteome of individual cumulus-oocyte complexes (COCs) with and without maturational competence, matured under in vivo or in vitro conditions (n = 5 per group). A novel, ultrasensitive mass spectrometry (MS) based protein profiling approach, using label-free quantification, was applied. The detected cumulus proteome included 2226 quantifiable proteins and was highly influenced by the maturation condition (479 differentially expressed proteins) as well as maturational competence of the corresponding oocyte (424 differentially expressed proteins). Enrichment analysis showed an overrepresentation of the complement and coagulation cascades (CCC), ECM-receptor interaction and steroid biosynthesis in cumulus of COCs that matured successfully under in vivo conditions. Verification of the origin of CCC proteins was achieved through detection of C3 secretion into the maturation medium, with significantly increasing concentrations from 12 (48.4 ng/ml) to 24 hours (68 ng/ml: p < 0.001). In relation, concentrations in follicular fluid, reflecting the in vivo situation, were >100x higher. In summary, this study identified important pathways that are impaired in IVM cumulus, as well as potential markers of the maturational competence of oocytes.
Ultrastructure imaging of Pseudomonas aeruginosa lawn biofilms and eradication of the tobramycin-resistant variants under in vitro electroceutical treatment
Authors: Lochab Varun , Jones Travis H. , Dusane Devendra H. , Peters Casey W. , Stoodley Paul , Wozniak Daniel J. , Subramaniam Vish V. , Prakash Shaurya 
Published: June 2020

AbstractElectrochemically generated bactericidal compounds have been shown to eradicate bacterial lawn biofilms through electroceutical treatment. However, the ultrastructure of biofilms exposed to these species has not been studied. Moreover, it is unknown if the efficacy of electroceutical treatment extends to antibiotic-resistant variants that emerge in lawn biofilms after antibiotic treatment. In this report, the efficacy of the in vitro electroceutical treatment of Pseudomonas aeruginosa biofilms is demonstrated both at room temperature and in an incubator, with a ~4 log decrease (p < 0.01) in the biofilm viability observed over the anode at both conditions. The ultrastructure changes in the lawn biofilms imaged using transmission electron microscopy demonstrate significant bacterial cell damage over the anode after 24 h of electroceutical treatment. A mix of both damaged and undamaged cells was observed over the cathode. Finally, both eradication and prevention of the emergence of tobramycin-resistant variants were demonstrated by combining antibiotic treatment with electroceutical treatment on the lawn biofilms.
Histologic tissue components provide major cues for machine learning-based prostate cancer detection and grading on prostatectomy specimens
Authors: Han Wenchao , Johnson Carol , Gaed Mena , Gómez José A. , Moussa Madeleine , Chin Joseph L. , Pautler Stephen , Bauman Glenn S. , Ward Aaron D. 
Published: June 2020

AbstractAutomatically detecting and grading cancerous regions on radical prostatectomy (RP) sections facilitates graphical and quantitative pathology reporting, potentially benefitting post-surgery prognosis, recurrence prediction, and treatment planning after RP. Promising results for detecting and grading prostate cancer on digital histopathology images have been reported using machine learning techniques. However, the importance and applicability of those methods have not been fully investigated. We computed three-class tissue component maps (TCMs) from the images, where each pixel was labeled as nuclei, lumina, or other. We applied seven different machine learning approaches: three non-deep learning classifiers with features extracted from TCMs, and four deep learning, using transfer learning with the 1) TCMs, 2) nuclei maps, 3) lumina maps, and 4) raw images for cancer detection and grading on whole-mount RP tissue sections. We performed leave-one-patient-out cross-validation against expert annotations using 286 whole-slide images from 68 patients. For both cancer detection and grading, transfer learning using TCMs performed best. Transfer learning using nuclei maps yielded slightly inferior overall performance, but the best performance for classifying higher-grade cancer. This suggests that 3-class TCMs provide the major cues for cancer detection and grading primarily using nucleus features, which are the most important information for identifying higher-grade cancer.
Extraction and reconstitution of membrane proteins into lipid nanodiscs encased by zwitterionic styrene-maleic amide copolymers
Authors: Fiori Mariana C. , Zheng Wan , Kamilar Elizabeth , Simiyu Geuel , Altenberg Guillermo A. , Liang Hongjun 
Published: June 2020

AbstractMembrane proteins can be reconstituted in polymer-encased nanodiscs for studies under near-physiological conditions and in the absence of detergents, but traditional styrene-maleic acid copolymers used for this purpose suffer severely from buffer incompatibilities. We have recently introduced zwitterionic styrene-maleic amide copolymers (zSMAs) to overcome this limitation. Here, we compared the extraction and reconstitution of membrane proteins into lipid nanodiscs by a series of zSMAs with different styrene:maleic amide molar ratios, chain sizes, and molecular weight distributions. These copolymers solubilize, stabilize, and support membrane proteins in nanodiscs with different efficiencies depending on both the structure of the copolymers and the membrane proteins.
Measurement of moisture-dependent ion diffusion constants in wood cell wall layers using time-lapse micro X-ray fluorescence microscopy
Authors: Jakes Joseph E. , Zelinka Samuel L. , Hunt Christopher G. , Ciesielski Peter , Frihart Charles R. , Yelle Daniel , Passarini Leandro , Gleber Sophie-Charlotte , Vine David , Vogt Stefan 
Published: June 2020

AbstractOur future bioeconomy depends on increased utilization of renewable lignocellulosic biomass. Controlling the diffusion of chemicals, such as inorganic ions, within secondary plant cell walls is central to many biomass applications. However, insufficient understanding of intra-cell-wall diffusion within secondary plant cell walls is hindering the advancement of many lignocellulosic biomass applications. In this work, X-ray fluorescence microscopy was used to measure diffusion constants of K+, Cu2+, and Cl− diffusing through loblolly pine (Pinus taeda) cell wall layers under 70%, 75%, or 80% relative humidity (RH). Results revealed that diffusion constants increased with RH, the larger Cu2+ diffused more slowly than the K+, and the Cl− diffusion constant was the same as that for the counter cation, indicating cations and anions diffused together to maintain charge neutrality. Comparison with electrical conductivity measurements showed that conductivity is being controlled by ion mobility over these RH. The results further support that intra-cell-wall diffusion of inorganic ions is a Fickian diffusion process occurring through rubbery amorphous polysaccharides, which contradicts previous assertions that intra-cell-wall diffusion is an aqueous process occurring through water pathways. Researchers can now utilize polymer science approaches to engineer the molecular architecture of lignocellulosic biomass to optimize properties for specific end uses.
Structural and enzymatic characterisation of the Type III effector NopAA (=GunA) from Sinorhizobium fredii USDA257 reveals a Xyloglucan hydrolase activity
Authors: Dorival Jonathan , Philys Sonia , Giuntini Elisa , Brailly Romain , de Ruyck Jérôme , Czjzek Mirjam , Biondi Emanuele , Bompard Coralie 
Published: June 2020

AbstractRhizobia are nitrogen-fixing soil bacteria that can infect legume plants to establish root nodules symbiosis. To do that, a complex exchange of molecular signals occurs between plants and bacteria. Among them, rhizobial Nops (Nodulation outer proteins), secreted by a type III secretion system (T3SS) determine the host-specificity for efficient symbiosis with plant roots. Little is known about the molecular function of secreted Nops (also called effectors (T3E)) and their role in the symbiosis process. We performed the structure-function characterization of NopAA, a T3E from Sinorhizobium fredii by using a combination of X-ray crystallography, biochemical and biophysical approaches. This work displays for the first time a complete structural and biochemical characterization of a symbiotic T3E. Our results showed that NopAA has a catalytic domain with xyloglucanase activity extended by a N-terminal unfolded secretion domain that allows its secretion. We proposed that these original structural properties combined with the specificity of NopAA toward xyloglucan, a key component of root cell wall which is also secreted by roots in the soil, can give NopAA a strategic position to participate in recognition between bacteria and plant roots and to intervene in nodulation process.
Effects of Age and Cochlear Implantation on Spectrally Cued Speech Categorization
Authors: DiNino Mishaela , Arenberg Julie G. , Duchen Anne L. R. , Winn Matthew B. 
Published: June 2020

Purpose
Weighting of acoustic cues for perceiving place-of-articulation speech contrasts was measured to determine the separate and interactive effects of age and use of cochlear implants (CIs). It has been found that adults with normal hearing (NH) show reliance on fine-grained spectral information (e.g., formants), whereas adults with CIs show reliance on broad spectral shape (e.g., spectral tilt). In question was whether children with NH and CIs would demonstrate the same patterns as adults, or show differences based on ongoing maturation of hearing and phonetic skills.

Method
Children and adults with NH and with CIs categorized a /b/–/d/ speech contrast based on two orthogonal spectral cues. Among CI users, phonetic cue weights were compared to vowel identification scores and Spectral-Temporally Modulated Ripple Test thresholds.

Results
NH children and adults both relied relatively more on the fine-grained formant cue and less on the broad spectral tilt cue compared to participants with CIs. However, early-implanted children with CIs better utilized the formant cue compared to adult CI users. Formant cue weights correlated with CI participants' vowel recognition and in children, also related to Spectral-Temporally Modulated Ripple Test thresholds. Adults and child CI users with very poor phonetic perception showed additive use of the two cues, whereas those with better and/or more mature cue usage showed a prioritized trading relationship, akin to NH listeners.

Conclusions
Age group and hearing modality can influence phonetic cue-weighting patterns. Results suggest that simple nonlexical categorization tests correlate with more general speech recognition skills of children and adults with CIs.
Directly comparing coronal and solar wind elemental fractionation
Authors: Stansby D. , Baker D. , Brooks D. H. , Owen C. J. 
Published: June 2020

Context. As the solar wind propagates through the heliosphere, dynamical processes irreversibly erase the signatures of the near–Sun heating and acceleration processes. The elemental fractionation of the solar wind should not change during transit, however, making it an ideal tracer of these processes.
Aims. We aim to verify directly if the solar wind elemental fractionation is reflective of the coronal source region fractionation, both within and across different solar wind source regions.
Methods. A backmapping scheme was used to predict where solar wind measured by the Advanced Composition Explorer (ACE) originated in the corona. The coronal composition measured by the Hinode Extreme ultraviolet Imaging Spectrometer (EIS) at the source regions was then compared with the in situ solar wind composition.
Results. On hourly timescales, there is no apparent correlation between coronal and solar wind composition. In contrast, the distribution of fractionation values within individual source regions is similar in both the corona and solar wind, but distributions between different sources have a significant overlap.
Conclusions. The matching distributions directly verify that elemental composition is conserved as the plasma travels from the corona to the solar wind, further validating it as a tracer of heating and acceleration processes. The overlap of fractionation values between sources means it is not possible to identify solar wind source regions solely by comparing solar wind and coronal composition measurements, but a comparison can be used to verify consistency with predicted spacecraft-corona connections.
The atomic gas of star-forming galaxies atz∼ 0.05 as revealed by the Five-hundred-meter Aperture Spherical Radio Telescope
Authors: Cheng Cheng , Ibar Edo , Du Wei , Molina Juan , Orellana-Gonzáles Gustavo , Zhang Bo , Zhu Ming , Xu Cong Kevin , Wu Shumei , Cao Tianwen , Huang Jia-Sheng , Leiton Roger , Hughes Thomas M. , He Chuan , Li Zijian , Xu Hai , Sophia Dai Y. , Shao Xu , Musin Marat 
Published: June 2020

Context.We report new H Iobservations of fourz ∼ 0.05 VALES galaxies undertaken during the commissioning phase of the Five-hundred-meter Aperture Spherical Radio Telescope (FAST).Aims.FAST is the largest single-dish telescope in the world, with a 500 m aperture and a 19-Beam receiver. Exploiting the unprecedented sensitivity provided by FAST, we aim to study the atomic gas content, via the H I21 cm emission line, in low-zstar formation galaxies taken from the Valparaíso ALMA/APEX Line Emission Survey (VALES). Together with previous Atacama Large Millimeter/submillimeter Array (ALMA) CO(J = 1−0) observations, the H Idata provides crucial information to measure the gas mass and dynamics.Methods.As a pilot H Igalaxy survey, we targeted four local star-forming galaxies atz ∼ 0.05. In particular, one of them has already been detected in H Iby the Arecibo Legacy Fast ALFA survey (ALFALFA), allowing a careful comparison. We use an ON-OFF observing approach that allowed us to reach an rms of 0.7 mJy beam−1at a 1.7 km s−1velocity resolution within only 20 min ON-target integration time.Results.In this Letter, we demonstrate the extraordinary capability of the FAST 19-beam receiver to push the detectability of the H Iemission line of extra-galactic sources. The H Iemission line detected by FAST shows good consistency with the previous Arecibo telescope ALFALFA results. Our observations are put into context with previous multi-wavelength data to reveal the physical properties of these low-zgalaxies. We find that the CO(J = 1−0) and H Iemission line profiles are similar. The dynamical mass estimated from the H Idata is an order of magnitude higher than the baryon mass and the dynamical mass derived from the CO observations, implying that the mass probed by dynamics of H Iis dominated by the dark matter halo. In one case, a target shows an excess of CO(J = 1−0) in the line centre, which can be explained by an enhanced CO(J = 1−0) emission induced by a nuclear starburst showing high-velocity dispersion.
Schädelfrakturen bei der Katze: CT-Befunde und Management
Authors: Betz Daniela Simon 
Published: June 2020

Knight R, Meeson RL. Feline head trauma: A CT analysis of skull fractures and their management in 75 cats. J Feline Med Surg 2019; 21: 1120–1126
Bis zu 27 % aller traumabedingten Frakturen bei Katzen betreffen die Schädelknochen. CT-Studien sind sensitiver in der Darstellung von Schädelfrakturen als die Röntgenuntersuchung. Ziel der Studie war daher, Frakturen bei der Katze nach Schädeltrauma mittels CT-Untersuchung zu charakterisieren und deren begleitende Verletzungen sowie das Management zu beschreiben.