bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular underpinnings of ssDNA specificity by Rep HUH-endonucleases and implications for HUH-tag multiplexing and engineering
Authors: Tompkins KJ , Houtti M , Litzau LA , Aird EJ , Everett BA , Nelson AT , Pornschloegl L , Limón-Swanson LK , Evans RL , Evans K , Shi K , Aihara H , Gordon WR 
Published: September 2020

AbstractReplication initiator proteins (Reps) from the HUH-endonuclease superfamily process specific single-stranded DNA (ssDNA) sequences to initiate rolling circle/hairpin replication in viruses, such as crop ravaging geminiviruses and human disease causing parvoviruses. In biotechnology contexts, Reps are the basis for HUH-tag bioconjugation and a critical adeno-associated virus genome integration tool. We solved the first co-crystal structures of Reps complexed to ssDNA, revealing a key motif for conferring sequence specificity and for anchoring a bent DNA architecture. In combination, we developed a deep sequencing cleavage assay, termed HUH-seq, to interrogate subtleties in Rep specificity and demonstrate how differences can be exploited for multiplexed HUH-tagging. Together, our insights allowed engineering of only four amino acids in a Rep chimera to predictably alter sequence specificity. These results have important implications for modulating viral infections, developing Rep-based genomic integration tools, and enabling massively parallel HUH-tag barcoding and bioconjugation applications.
Successful thesis proposals in architecture and urban planning
Authors: Abdellatif Mahmoud , Abdellatif Reham 
Published: September 2020

PurposeThe purpose of this research is to improve the understanding of what constitutes a successful thesis proposal (TP) and as such enhance the quality of the TP writing in architecture, planning and related disciplines.Design/methodology/approachBased on extended personal experience and a review of relevant literature, the authors proposed a conception of a successful TP comprising 13 standard components. The conception provides specific definition/s, attributes and success rules for each component. The conception was applied for 15 years on several batches of Saudi graduate students. The implications of the conception were assessed by a students' opinion survey. An expert inquiry of experienced academics from architectural schools in nine countries was applied to validate and improve the conception.FindingsAssessment of the proposed conception demonstrated several positive implications on students' knowledge, performance and outputs which illustrates its applicability in real life. Experts' validation of the conception and constructive remarks have enabled further improvements on the definitions, attributes and success rules of the TP components.Research limitations/implicationsThe proposed TP conception with its 13 components is limited to standard problem-solving research and will differ in the case of other types such as hypothesis-based research.Practical implicationsThe proposed conception is a useful directive and evaluative tool for writing and assessing thesis proposals for graduate students, academic advisors and examiners.Social implicationsThe research contributes to improving the quality of thesis production process among the academic community in the built environment fields.Originality/valueThe paper is meant to alleviate the confusion and hardship caused by the absence of a consensus on what constitutes a successful TP in the fields of architecture, urban planning and related disciplines.
Perceived benefits and issues associated with COBie datasheet handling in the construction industry
Authors: Kumar Vishal , Teo Evelyn Ai Lin 
Published: September 2020

Purpose
Until now, the usage and usability factors of Construction Operation Building information exchange (COBie) datasheet have remained largely overlooked. This oversight may be the potential factor in the lower adoption rates as well as the effective usage of COBie datasheet in the architecture, engineering and construction-facilities management industry. The purpose of this study is to investigate the benefits and key issues associated with COBie datasheet handling and identify the key technological solutions, which can help in mitigating the identified issues.

Design/methodology/approach
A literature review was conducted to identify the key benefits of using COBie and issues, which are associated with COBie datasheet handling. This paper has also designed a questionnaire based on a literature review and surveyed professionals who are well versed with handling COBie datasheet. Using responses, the issues are analyzed and discussed using non-parametric statistical analysis.

Findings
A total of 9 key benefits and 24 key issues categorized under three groups of usability issues, technical issues and organizational/other issues were identified. The results from the survey agree with all the key issues associated with COBie datasheet handling (with 86 responses). This research also proposes key ideas, that can help in mitigating these issues.

Originality/value
There is a paucity in published literature, which discusses in detail about the various issues associated with COBie datasheet handling. This research study aims to address this gap by identifying key issues by looking at the entire COBie data-capturing process holistically. Finding from this study can help professionals to understand these issues and develop appropriate technological solutions, which can make COBie data capturing and understanding easier. The findings could also assist in increasing the adoption rate of COBie, which could be achieved through mitigation of identified issues.
Towards sustainable tourism: an empirical investigation
Authors: Garg Prerna , Pandey Anoop 
Published: September 2020

Purpose
Looking at the paucity of research examining behaviour in reference to sustainable tourism in India, the study aims to propose a framework for predicting the mediating role played by personal norms in determining the intention to adopt sustainable tourism in India.

Design/methodology/approach
The study is cross-sectional in nature and has gathered responses from 275 valid respondents with diverse socio-demographic profiles. EFA was conducted, followed by CFA and structural model analysis to test the hypothesized relationships.

Findings
The results indicate that all the hypotheses were found to be significant, thus confirming the direct and indirect effect of consumer knowledge and consumer perceived effectiveness on an intention to adopt sustainable tourism.

Research limitations/implications
The study has not just added a different perspective to sustainable tourism but has also attempted to capture the attention of all academicians and practitioners who have sidelined this relevant concept in Indian context.

Practical implications
Marketers aiming to alter tourist’s behaviour with respect to sustainability must understand that they need to redesign their strategies within the context of personal norms or values.

Originality/value
While some of the literature has attempted to explore the attitudinal dimension of environmentally conscious behaviour, there is much scope to examine the factors motivating the formation of intention towards sustainable tourism in Indian context. The confined use of constructs from value-belief-norm model and theory of planned behaviour in sustainable tourism, has led to the development of the proposed research framework in the study.
Illegal gold mining in Indonesia: structure and causes
Authors: Hasibuan Ongku P. , Tjakraatmadja Jann H. , Sunitiyoso Yos 
Published: September 2020

PurposeThis study aims to understand the process, structure and determinants of persistent illegal gold mining in Indonesia.Design/methodology/approachAn exploratory study included a literature review, field observations and in-depth interviews. Fieldwork was conducted at two mining locations in Kalimantan, Indonesia. Sixteen respondents representing key stakeholders: government, mining companies, mining associations and community, including illegal miners, were interviewed. The findings of the literature review were categorized as the push or pull factors within the topics, and field observations verified the literature review results regarding Indonesia. Interview data were analyzed using content analysis methods. A structural diagram of the actors and causal loop diagrams among the determinants of illegal gold mining was created and interpreted.FindingsA complex activity process and structure were revealed involving a range of actors. Politicians, government officials and law enforcement authorities added the complexity to the structure. Six main determinants of persistent illegal gold mining emerged: financial, low entry barrier, regulations and policies, supporting resources availability, politics and power and psychosocial factors.Practical implicationsThis study provides a deeper understanding for policymakers to consider when formulating policies on natural resources, and insights for mining companies to minimize conflicts with the community.Originality/valueThe study contributes to our knowledge of the complex structure and main determinants of illegal gold mining in Indonesia, which supports efforts to curtail this illegal activity.
Climate vulnerability as a catalyst for early stadium replacement
Authors: Kellison Timothy , Orr Madeleine 
Published: September 2020

PurposeSevere hazards associated with climate change are threatening human settlements, thereby requiring global cities to implement comprehensive climate adaptation strategies. For sports organizations, adaptive measures may include designing and constructing new stadiums. In this study, the authors explore climate change as a vehicle for urban transformation, particularly as it relates to the replacement of existing stadiums with new, more sustainable and resilient venues.Design/methodology/approachThe authors employed a collective case study approach focusing on three recent cases of stadium replacement: Globe Life Field in Arlington, Texas; Oakland Ballpark in Oakland, California; and Marlins Park in Miami, Florida. These cases were selected because an official representative of each team made explicit references to some form of climate adaptation, though each ballpark faces a distinctive climate-related threat.FindingsEach of the cases illustrates the various ways in which climate vulnerability may be deployed by teams and policymakers to replace professional sports stadiums. Although all three examples involved the replacement of an existing ballpark, only in the Texas case was climate adaptation openly cited as the primary reason for stadium replacement. Still, ballpark replacement plans in Oakland and Miami included significant and costly design features to protect the stadiums from extreme weather events.Originality/valueThis study applies the concept of climate vulnerability to illustrate a potential strategy to justify stadium replacement. As cities and metropolitan regions continue to grapple with the grand challenge of climate change, the associated vulnerability of large public assembly facilities such as major sports stadiums – particularly those prominently situated in urban centers – can no longer be ignored.
The role of co-creation experience in engaging customers with service brands
Authors: Hussain Khalid , Jing Fengjie , Junaid Muhammad , Zaman Qamar Uz , Shi Huayu 
Published: September 2020

Purpose
This study aims to investigate the outcomes of customers’ co-creation experience in a realistic and routinely performed co-creation setting, a restaurant. To fulfill this purpose, the current study links the branding literature to hospitality research and offers a novel framework by incorporating customers’ co-creation experience, customer brand engagement, emotional brand attachment and customer satisfaction in an integrated research model.

Design/methodology/approach
Data were collected from 421 diners at Chinese hotpot restaurants via a self-administered questionnaire. The reliability and convergent and discriminant validities were established through confirmatory factor analysis, and then hypotheses were tested through structural equation modeling.

Findings
This study demonstrates that customers’ co-creation experience with a restaurant brand positively impacts customer brand engagement, emotional brand attachment and customer satisfaction. In addition, current study examines these relational paths at the dimensional level by taking the co-creation experience and customer brand engagement as multidimensional constructs. The resulting in-depth investigation reveals that the hedonic, social and economic experience dimensions of co-creation experience positively influence customer satisfaction, emotional brand attachment and customer brand engagement’s buying, referring, influencing and feedback dimensions.

Practical implications
This study helps relationship and brand managers better understand customer experience in co-creation settings and paves the way for managers to devise engagement strategies.

Originality/value
The current study marks an initial attempt to delineate the outcomes of customers’ co-creation experience in a realistic co-creation setting. Furthermore, the study is first of its kind that investigates the relationship of co-creation experience and customer brand engagement at the dimensional level.
Ethical leadership and newcomer adjustment: examining a moderated mediation model
Authors: Kang Fei , Zhang Han 
Published: September 2020

PurposeSubstantial research has examined the pivotal role of ethical leadership in generating employee outcomes. To date, though, little is known about the relationship between ethical leadership and newcomers' adjustment. The purpose of this paper is to contribute to this gap by examining the effect of ethical leadership on newcomers' adjustment. In doing so, the authors highlighted positive emotions of newcomers as a mediating mechanism that explains the aforementioned association.Design/methodology/approachData were drawn from a two-wave sample of 271 newcomers. The hypotheses were tested by using hierarchical regression analyses and Hayes' PROCESS macro.FindingsThe results demonstrated that ethical leadership affects employees' positive emotions which, in turn, positively relates to newcomers' adjustment. Additionally, newcomers' social comparison orientation moderated the effect of ethical leadership on newcomers' positive emotions.Research limitations/implicationsThe research uses a correlational research design, making it difficult to derive causal inferences from the data. Moreover, the data we obtained on the variables were all based on employees' self-reports, which might inflate the relationship between some of the variables.Originality/valueTo the best of the knowledge, this is the first study that illustrates the role of ethical leadership in enhancing both newcomers’ positive emotions and adjustment.
Loyalty layers, expectations and the role of knowledge
Authors: Quach Sara , Thaichon Park , Roberts Robin E. , Weaven Scott 
Published: September 2020

PurposeAdopting exchange theory and social orientation of loyalty, this research investigates the antecedents of customer loyalty consisting of (1) loyalty layers (i.e. personal loyalty, relationship with consumption communities and local network effects) and (2) loyalty expectations (i.e. service quality, reciprocity and firm innovativeness) and how these relationships are moderated by customer knowledge.Design/methodology/approachThe data were collected from 4,208 customers in the mobile services industry using mall intercept technique.FindingsThe findings reveal that loyalty layers, including personal loyalty and relationship with consumption communities, can influence customers' expectations of service providers. The degree to which the firm is able to handle and meet customers' expectations over time would result in the strength of customer loyalty. Customer expectations also mediate the relationships between different loyalty layers and customer loyalty. In addition, customer knowledge significantly moderates the effects of loyalty layers and expectations on both attitudinal loyalty and behavioural loyalty.Originality/valueThe study extends the current body of knowledge by incorporating a sociological perspective to examine the relationships between loyalty layers and customer expectations and customer loyalty. This research enables service operators to establish strategies to sustain customer loyalty across different customer segments with various levels of knowledge.
Help or resistance? Product market competition and water information disclosure: evidence from China
Authors: Zhou Zhifang , Zhang Tao , Chen Jiachun , Zeng Huixiang , Chen Xiaohong 
Published: September 2020

Purpose
This paper investigates the relationship between product market competition and firms’ water information disclosure and how firms’ ownership type can affect this relationship in China, offering new insights into corporate water management.

Design/methodology/approach
The authors investigated 303 Chinese listed companies in highly water-sensitive industries to examine how product market competition influences corporate water information disclosure by subdividing the product market competition into market competition at the firm level and the industry competition intensity at the industry level.

Findings
The results show that there exists an inverted U-shaped relationship between industry competition and water information disclosure; enterprises with the highest market power in a mildly competitive industry are more willing to voluntarily disclose water information and play an industry benchmarking role. Further tests demonstrate that the relationship between industry competition intensity and water information disclosure is stronger for state-owned enterprises than for private enterprises.

Research limitations/implications
The current water resources regulations in China are relatively lax and the water risk awareness of firms is weak, which may affect the applicability of the results. In addition, water information disclosure research is a relatively new field and a quantitative index system for water information disclosure is still in the exploratory stage. Further developments, including the selection, definition and measuring methods of a water index are required.

Practical implications
The authors developed a new direction of enterprise water management activities from the perspective of market competition. Based on the market conditions in China, the authors also investigated the impact of the ownership type of the enterprises on the relationship between market competition and water information disclosure.

Social implications
The authors suggested that the government should improve laws and regulations and adopt incentive mechanisms to encourage enterprises to implement water resource management. In addition, the government should encourage high market status enterprises to actively fulfill their environmental responsibilities so that the entire industry is encouraged to follow suit.

Originality/value
This study represents an important development in the field of environmental accounting and is the first research on corporate water information disclosure; it also extends the research on the influence mechanisms of market competition on the environmental management practices of enterprises.
Infrastructures éducatives et achèvement des études en contexte camerounais de décentralisation
Authors: Fouelefack Perrier Carmelle , Nembot Ndeffo Luc , Maiga Windkouni Haoua Eugenie 
Published: September 2020

AbstractThe objective of the article is to measure the impact in 2014 of school infrastructures on school completion in Cameroon's rural milieu. They are provided between 2002 and 2012 through a participatory development model that the National Community‐Driven Development Programme (NCDDP) is implementing in local governments to fight against school drop‐out, a major obstacle to education‐for‐all in Cameroon. Applying a triple difference to data from The fourth Cameroonian Household Survey (ECAM4), it emerges that with a classroom built or equipped, the number of years of completed schooling by children aged 2 to 6 in 2004 has increased on average by 0.004 compared to that of those aged 12 to 16 in 2004 who dropped out of school in the same year. This leads to recommendations to consolidate the participatory development model to ensure better results from the decentralization of education.
Resource Extraction, Economic Growth, and the Climate Dilemma in Canada and Australia
Authors: MacDonald Connor , Sloman Peter 
Published: September 2020

AbstractCanada and Australia are two of the economic success stories of the last thirty years, enjoying rapid growth during the 1990s and 2000s and (unlike the UK and US) escaping the worst effects of the 2008 financial crisis. In both countries, however, economic growth has become highly dependent on commodities extraction, leaving them vulnerable to fluctuations in commodity prices, and imposing political constraints on tackling climate change. This article explores the economic and political challenges which the role of the natural resource sector has posed in Australia and Canada in recent years, and examines the contrasting ways in which Scott Morrison and Justin Trudeau’s governments deployed the climate issue in the two countries’ 2019 federal elections.
The Italian State, its Regions and the Virus
Authors: Rubinelli Lucia 
Published: September 2020

AbstractItalian politics has always been characterised by deep regional divides. While the north and centre are well integrated into the European economy, southern regions struggle to keep up their productivity and employment levels. Although this divide has a long history, the Covid‐19 crisis is set to worsen the inequality separating the two parts of the country. This article looks at how the coronavirus pandemic immediately assumed a regional dimension, which was reflected not only in the geography of the contagion, but also in how the central state struggled to manage the contrasting demands coming from northern and southern regions. Although the north has been the epicentre of the health crisis, the south is set to pay the highest economic and social price for the lockdown measures. The conclusion suggests that, while the Covid crisis has confirmed the salience of regional divides for Italian politics, it might also strengthen autonomist forces.
Sustaining What is Valuable: Contours of an Educational Language About Values
Authors: Bergdahl Lovisa , Langmann Elisabet 
Published: September 2020

Abstract
Making liberal democratic values meaningful to study in schools is a more complex issue than being a question of turning values into explicit educational goals (Schleicher) or of curing a motivational deficit (Critchley). Since values seem to play an important role in the practices and commitments of people's everyday lives, values are calling for a continual refinement of our words in relation to the world (Laverty). The purpose of this article is to offer contours of an educational language about values that acknowledges this refinement and the pedagogical work that teachers might do—by way of language—in order to sustain the living-on of what is valued and valuable to us as individuals and as societies. To this end, the article is divided into two parts. The first part takes the temperature of the current political and educational debates, offering thereby a sociopolitical background to the need of a renewed language about values. Drawing on ordinary language philosophy (Moi, Murdoch and Forsberg) and the idea that there is an intimate relationship between how we look at the world (attention) and the words we use in describing it (language). The second part of the article places the emergence of values in a particular time in history before suggesting a more existential vocabulary about values for the purpose of teaching values in schools.