bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of swine enteroids as in vitro models forLawsonia intracellularisinfection1,2
Authors: Resende Talita Pilar , Medida Ramya Lekha , Vannucci Fabio A , Saqui-Salces Milena , Gebhart Connie 
Published: January 2020

AbstractThe enteric pathogen Lawsonia intracellularis is one of the main causes of diarrhea and compromised weight gain in pigs worldwide. Traditional cell-line cultures have been used to study L. intracellularis pathogenesis. However, these systems fail to reproduce the epithelial changes observed in the intestines of L. intracellularis-infected pigs, specifically, the changes in intestinal cell constitution and gene expression. A more physiologically accurate and state-of-the-art model is provided by swine enteroids derived from stem cell-containing crypts from healthy pigs. The objective of this study was to verify the feasibility of two-dimensional swine enteroids as in vitro models for L. intracellularis infection. We established both three- and two-dimensional swine enteroid cultures derived from intestinal crypts. The two-dimensional swine enteroids were infected by L. intracellularis in four independent experiments. Enteroid-infected samples were collected 3 and 7 d postinfection for analysis using real-time quantitative PCR and L. intracellularis immunohistochemistry. In this study, we show that L. intracellularis is capable of infecting and replicating intracellularly in two-dimensional swine enteroids derived from ileum.
Ingestion of organic acids and cinnamaldehyde improves tissue homeostasis of piglets exposed to enterotoxic Escherichia coli (ETEC)
Authors: Jiménez Milton J , Berrios Roger , Stelzhammer Sabine , Hohmann Miriam , Verri Waldiceu , Bracarense Ana Paula F R L 
Published: January 2020

Abstract
Organic acids (OA) and phytogenic compounds have been used in pig feeding as alternatives to antibiotic growth promoters. However, few studies have evaluated the systemic effect of the combination of these additives. The aim of this study was to assess the impact of an organic acid-based feed additive (OAFA), containing a blend of OA and cinnamaldehyde, on the tissue integrity of bacterially challenged piglets. Thirty weaned piglets 21 d old were used in a 19-d trial. Pigs received a standard diet during the first 7 d and afterward were allotted to five treatments. Dietary treatments were: Control (basal diet), Escherichia coli (basal diet and challenge with E. coli), colistin (basal diet + 200 mg colistin/kg feed + challenge with E. coli), OAFA1 (basal diet + 1 kg OAFA/ton feed + challenge with E. coli), and OAFA2 (basal diet + 2 kg OAFA/ton feed + challenge with E. coli). Seven days after the beginning of the treatment, the animals were challenged with an enterotoxic strain of E. coli (K88) for pigs. Five days after the challenge, all animals were euthanized for tissue sampling for histological and oxidative stress (intestine and liver) analysis. The reduced glutathione (GSH), ferric-reducing ability potential (FRAP), and free-radical scavenging ability (ABTS) assays were used to evaluate the intestinal antioxidant defense. Lipid peroxidation and superoxide anion production were evaluated through the levels of thiobarbituric acid-reactive substances (TBARS) and nitroblue tetrazolium (NBT) reduction assay, respectively. Animals fed the OAFA (1 and 2) diets had a decrease (P &lt; 0.05) on histological changes in the intestine, liver, mesenteric lymph nodes, and spleen. Greater villus height (VH) and a higher ratio of VH to crypt depth (CD) were observed in animals of the OAFA2 group compared with the control and E. coli groups. The colistin and OAFA groups decreased (P &lt; 0.05) the number of inflammatory cells in intestinal lamina propria. OAFA2 group increased (P &lt; 0.05) intestinal cell proliferation. Colistin and OAFA2 supplementation induced a decrease (P &lt; 0.05) in the levels of TBARS in both the intestine and liver compared with the E. coli group. In addition, an increase (P &lt; 0.05) in GSH and FRAP ileal levels was observed in the OAFA2 group compared with E. coli group. These results show that the supplementation with OAFA in the diet of weaned piglets, especially at a dose of 2 kg/ton (OAFA2) protected tissues against enterotoxigenic Escherichia coli (ETEC) damage.
Telemammography: Technical Advances Improve Patient Access in Breast Care
Authors: Chung Hannah L , Parikh Jay R 
Published: January 2020

Abstract
Screening mammography’s efficacy in reducing breast cancer deaths depends on patient compliance with screening recommendations and the radiologist’s interpretative skills. Reasons for suboptimal screening compliance may be multifactorial, including possible limitations in access. Additionally, while studies show experienced breast radiologists are more accurate in their mammographic interpretation, only a minority of the nation’s mammograms are interpreted by breast imaging specialists. To simultaneously optimize the benefit of early breast cancer detection while minimizing the harms associated with a false positive interpretation, delivery models that help improve access to breast expertise should be considered. Telemammography is one such delivery model that may be underutilized in current practice. While radiologists and other stakeholders of healthcare have accepted teleradiology interpretation of non-mammography studies as routine, telemammography use and acceptance is less well known. In this article, we review the operational components of a telemammography practice in today’s information- and technology-dependent society. Current use of telemammography and remaining potential challenges are discussed. Telemammography can improve healthcare delivery and access by bringing together patients and breast expertise. If accepted, use of telemammography can help meet Centers for Disease Control’s Healthy People 2020 goals related to breast cancer.
Relative Abundance of the Stink Bug (Hemiptera: Pentatomidae) Complex Infesting Rice in the Everglades Agricultural Area of Florida
Authors: VanWeelden Matthew T , Cherry Ronald H , Karounos Michael 
Published: January 2020

AbstractFlorida’s rice stink bug complex comprises three species; Oebalus pugnax (F.), O. insularis (Stal), and O. ypsilongriseus (DeGeer), the latter two of which are invasive and exclusive to Florida within the United States. A series of surveys were conducted in 2017 and 2018 to determine the relative abundance of the three species throughout Florida’s rice growing region within the Everglades Agricultural Area, in addition to comparing their seasonality within crop and noncrop habitats. Sampling occurred in commercial rice fields and adjacent transects of graminaceous noncrop hosts using sweep nets. Oebalus pugnax (52.7%) and O. insularis (61.7%) were the most abundant species in 2017 and 2018, respectively. Both species were more prevalent in rice fields compared to transects of noncrop hosts. Oebalus ypsilongriseus remained in low abundance relative to O. pugnax and O. insularis, and did not differ in numbers collected among rice and noncrop hosts. Of the noncrop hosts in transects, Panicum dichotomiflorum (fall panicum) was the most abundant across both years. This study is the first report of Oebalus species feeding on Echinochloa crus-galli (common barnyardgrass) in Florida. This study shows that the invasive O. insularis continues to increase in abundance, and has surpassed O. pugnax in terms of regional populations. These results emphasize the need for additional studies to assess the interactions among O. insularis and other Oebalus species in addition to its feeding behavior in Florida rice.
Does pain and hip function improve 2 years after reverse periacetabular osteotomy? A follow-up study of 74 patients
Authors: Bræmer Christina Nielsen , Langberg Sarah Ankjær , Jakobsen Stig Storgaard , Jakobsen Søren Reinhold , Aleixo Hugo , Søballe Kjeld , Mechlenburg Inger 
Published: January 2020

AbstractData on outcome in patients with acetabular retroversion (AR) treated with reverse periacetabular osteotomy (reverse PAO) are sparse. The aim of the study was to investigate changes in pain and hip function among patients with AR 2 years after reverse PAO and to examine whether changes in pain were associated with changes in hip function. In addition, to evaluate patient satisfaction and changes in quality of life (QoL). We present a prospective follow-up study with patient-reported outcome data from Aarhus University Hospital in Denmark. Pain at rest and during activity was measured with a Visual Analogue Scale (VAS), hip function with the Hip disability and Osteoarthritis Outcome Score (HOOS) and QoL with the Short-Form 36, pre-operatively and 2 years after reverse PAO in 74 patients. Changes were analysed using paired t-test and multiple linear regressions. Significant and clinically relevant mean improvements in pain and hip function were found. The numbers of responders achieving a minimal clinically important difference varied from 51 to 73%. Positive significant association between changes in pain and changes in hip function were found. Significant mean improvement in QoL was found. The study had a loss to follow-up of 23%. Two years after reverse PAO, patients diagnosed with AR showed significant and clinically relevant mean improvements in pain and hip function. Decreased pain was significantly associated with improved hip function. The majority of patients were satisfied with the result of surgery and QoL was similar to the Danish background population.
Usefulness of carbon-11-labeled methionine positron-emission tomography for assessing the treatment response of primary central nervous system lymphoma
Authors: Miyakita Yasuji , Ohno Makoto , Takahashi Masamichi , Kurihara Hiroaki , Katai Hitoshi , Narita Yoshitaka 
Published: January 2020

Abstract

Background
Primary central nervous system lymphoma (PCNSL) responds relatively quickly to chemotherapy or radiotherapy. However, determination of a complete response after treatment is often difficult because of extremely light residual contrast enhancement on magnetic resonance images due to the effects of microhemorrhages and scar tissue formation. These small enhancing lesions define an unconfirmed complete response. The aim of this study was to investigate the usefulness of carbon-11-labeled methionine (11C-Met) positron-emission tomography (PET) for determining the treatment response of PCNSL.

Methods
Data for 36 patients who were treated for PCNSL between 2011 and 2015 and underwent magnetic resonance imaging and 11C-Met PET were reviewed. Magnetic resonance imaging findings were classified as complete response, unconfirmed complete response, and tumor mass (a composite of partial response, stable disease and progressive disease). PET images were evaluated, standardized uptake values were quantified, and the tumor-to-normal tissue count ratio (TNR) was calculated. Receiver operating characteristic curves were generated to determine the optimal cutoff TNRs.

Results
The optimal TNRs for differentiating complete response and unconfirmed complete response from tumor mass were 1.83 (area under the curve, 0.951) and 1.80 (area under the curve, 0.932), respectively. The corresponding sensitivity and specificity values for the diagnosis of tumor mass were 82.4 and 100%, respectively, in the complete response group and 85.3 and 85%, respectively, in the unconfirmed complete response group.

Conclusions
A TNR of ≥1.80 can aid in the detection of active PCNSL using 11C-Met PET. Thus, 11C-Met-PET may be a useful tool for accurate evaluation of the treatment efficacy in PCNSL.
The impact of AGN feedback on galaxy intrinsic alignments in the Horizon simulations
Authors: Soussana Adam , Chisari Nora Elisa , Codis Sandrine , Beckmann Ricarda S , Dubois Yohan , Devriendt Julien , Peirani Sebastien , Laigle Clotilde , Pichon Christophe , Slyz Adrianne 
Published: January 2020

ABSTRACT
The intrinsic correlations of galaxy shapes and orientations across the large-scale structure of the Universe are a known contaminant to weak gravitational lensing. They are known to be dependent on galaxy properties, such as their mass and morphologies. The complex interplay between alignments and the physical processes that drive galaxy evolution remains vastly unexplored. We assess the sensitivity of intrinsic alignments (shapes and angular momenta) to active galactic nuclei (AGN) feedback by comparing galaxy alignment in twin runs of the cosmological hydrodynamical Horizon simulation, which do and do not include AGN feedback, respectively. We measure intrinsic alignments in three dimensions and in projection at $z$ = 0 and $z$ = 1. We find that the projected alignment signal of all galaxies with resolved shapes with respect to the density field in the simulation is robust to AGN feedback, thus giving similar predictions for contamination to weak lensing. The relative alignment of galaxy shapes around galaxy positions is however significantly impacted, especially when considering high-mass ellipsoids. Using a sample of galaxy ‘twins’ across simulations, we determine that AGN changes both the galaxy selection and their actual alignments. Finally, we measure the alignments of angular momenta of galaxies with their nearest filament. Overall, these are more significant in the presence of AGN as a result of the higher abundance of massive pressure-supported galaxies.
The observational anatomy of externally photoevaporating planet-forming discs – I. Atomic carbon
Authors: Haworth Thomas J , Owen James E 
Published: January 2020

ABSTRACT
We demonstrate the utility of C i as a tracer of photoevaporative winds that are being driven from discs by their ambient UV environment. Commonly observed CO lines only trace these winds in relatively weak UV environments and are otherwise dissociated in the wind at the intermediate to high UV fields that most young stars experience. However, C i traces unsubtle kinematic signatures of a wind in intermediate UV environments (∼1000 G0) and can be used to place constraints on the kinematics and temperature of the wind. In C i position–velocity (PV) diagrams external photoevaporation results in velocities that are faster than those from Keplerian rotation alone, as well as emission from quadrants of PV space in which there would be no Keplerian emission. This is independent of viewing angle because the wind has components that are perpendicular to the azimuthal rotation of the disc. At intermediate viewing angles (∼30–60°) moment 1 maps also exhibit a twisted morphology over large scales (unlike other processes that result in twists, which are typically towards the inner disc). C i is readily observable with ALMA, which means that it is now possible to identify and characterize the effect of external photoevaporation on planet-forming discs in intermediate UV environments.
Classification of pulsars with Dirichlet process Gaussian mixture model
Authors: Ay Fahrettin , İnce Gökhan , Kamaşak Mustafa E , Ekşi K Yavuz 
Published: January 2020

ABSTRACT
Young isolated neutron stars (INSs) most commonly manifest themselves as rotationally powered pulsars that involve conventional radio pulsars as well as gamma-ray pulsars and rotating radio transients. Some other young INS families manifest themselves as anomalous X-ray pulsars and soft gamma-ray repeaters that are commonly accepted as magnetars, i.e. magnetically powered neutron stars with decaying super-strong fields. Yet some other young INSs are identified as central compact objects and X-ray dim isolated neutron stars that are cooling objects powered by their thermal energy. Older pulsars, as a result of a previous long episode of accretion from a companion, manifest themselves as millisecond pulsars and more commonly appear in binary systems. We use Dirichlet process Gaussian mixture model (DPGMM), an unsupervised machine learning algorithm, for analysing the distribution of these pulsar families in the parameter space of period and period derivative. We compare the average values of the characteristic age, magnetic dipole field strength, surface temperature, and transverse velocity of all discovered clusters. We verify that DPGMM is robust and provide hints for inferring relations between different classes of pulsars. We discuss the implications of our findings for the magnetothermal spin evolution models and fallback discs.
Differential evolution in 3′UTRs leads to specific gene expression in Staphylococcus
Authors: Menendez-Gil Pilar , Caballero Carlos J , Catalan-Moreno Arancha , Irurzun Naiara , Barrio-Hernandez Inigo , Caldelari Isabelle , Toledo-Arana Alejandro 
Published: January 2020

Abstract
The evolution of gene expression regulation has contributed to species differentiation. The 3′ untranslated regions (3′UTRs) of mRNAs include regulatory elements that modulate gene expression; however, our knowledge of their implications in the divergence of bacterial species is currently limited. In this study, we performed genome-wide comparative analyses of mRNAs encoding orthologous proteins from the genus Staphylococcus and found that mRNA conservation was lost mostly downstream of the coding sequence (CDS), indicating the presence of high sequence diversity in the 3′UTRs of orthologous genes. Transcriptomic mapping of different staphylococcal species confirmed that 3′UTRs were also variable in length. We constructed chimeric mRNAs carrying the 3′UTR of orthologous genes and demonstrated that 3′UTR sequence variations affect protein production. This suggested that species-specific functional 3′UTRs might be specifically selected during evolution. 3′UTR variations may occur through different processes, including gene rearrangements, local nucleotide changes, and the transposition of insertion sequences. By extending the conservation analyses to specific 3′UTRs, as well as the entire set of Escherichia coli and Bacillus subtilis mRNAs, we showed that 3′UTR variability is widespread in bacteria. In summary, our work unveils an evolutionary bias within 3′UTRs that results in species-specific non-coding sequences that may contribute to bacterial diversity.
Secondary nucleotide messenger c-di-GMP exerts a global control on natural product biosynthesis in streptomycetes
Authors: Makitrynskyy Roman , Tsypik Olga , Nuzzo Desirèe , Paululat Thomas , Zechel David L , Bechthold Andreas 
Published: January 2020

Abstract
Cyclic dimeric 3′-5′ guanosine monophosphate, c-di-GMP, is a ubiquitous second messenger controlling diverse cellular processes in bacteria. In streptomycetes, c-di-GMP plays a crucial role in a complex morphological differentiation by modulating an activity of the pleiotropic regulator BldD. Here we report that c-di-GMP plays a key role in regulating secondary metabolite production in streptomycetes by altering the expression levels of bldD. Deletion of cdgB encoding a diguanylate cyclase in Streptomycesghanaensis reduced c-di-GMP levels and the production of the peptidoglycan glycosyltransferase inhibitor moenomycin A. In contrast to the cdgB mutant, inactivation of rmdB, encoding a phosphodiesterase for the c-di-GMP hydrolysis, positively correlated with the c-di-GMP and moenomycin A accumulation. Deletion of bldD adversely affected the synthesis of secondary metabolites in S. ghanaensis, including the production of moenomycin A. The bldD-deficient phenotype is partly mediated by an increase in expression of the pleiotropic regulatory gene wblA. Genetic and biochemical analyses demonstrate that a complex of c-di-GMP and BldD effectively represses transcription of wblA, thus preventing sporogenesis and sustaining antibiotic synthesis. These results show that manipulation of the expression of genes controlling c-di-GMP pool has the potential to improve antibiotic production as well as activate the expression of silent gene clusters.
Ferrimagnetic mPEG-b-PHEP copolymer micelles loaded with iron oxide nanocubes and emodin for enhanced magnetic hyperthermia–chemotherapy
Authors: Song Yonghong , Li Dongdong , Lu Yang , Jiang Kun , Yang Yi , Xu Yunjun , Dong Liang , Yan Xu , Ling Daishun , Yang Xianzhu , Yu Shu-Hong 
Published: January 2020

Abstract
As a non-invasive therapeutic method without penetration-depth limitation, magnetic hyperthermia therapy (MHT) under alternating magnetic field (AMF) is a clinically promising thermal therapy. However, the poor heating conversion efficiency and lack of stimulus–response obstruct the clinical application of magnetofluid-mediated MHT. Here, we develop a ferrimagnetic polyethylene glycol-poly(2-hexoxy-2-oxo-1,3,2-dioxaphospholane) (mPEG-b-PHEP) copolymer micelle loaded with hydrophobic iron oxide nanocubes and emodin (denoted as EMM). Besides an enhanced magnetic resonance (MR) contrast ability (r2 = 271 mM−1 s−1) due to the high magnetization, the specific absorption rate (2518 W/g at 35 kA/m) and intrinsic loss power (6.5 nHm2/kg) of EMM are dozens of times higher than the clinically available iron oxide nanoagents (Feridex and Resovist), indicating the high heating conversion efficiency. Furthermore, this composite micelle with a flowable core exhibits a rapid response to magnetic hyperthermia, leading to an AMF-activated supersensitive drug release. With the high magnetic response, thermal sensitivity and magnetic targeting, this supersensitive ferrimagnetic nanocomposite realizes an above 70% tumor cell killing effect at an extremely low dosage (10 μg Fe/mL), and the tumors on mice are completely eliminated after the combined MHT–chemotherapy.
Recurrent Giant Anterior Cerebral Artery (ACA) Aneurysm With Previous Coiling, Attempted Clipping Then Direct ACA-to-ACA Bypass: 2-Dimensional Operative Video
Authors: Hendricks Benjamin K , Spetzler Robert F 
Published: January 2020

Abstract
Giant aneurysms are defined as lesions with a widest diameter of 2.5 cm or greater and account for 2% to 5% of all intracranial aneurysms. These lesions are challenging entities for microsurgical management with techniques such as direct aneurysmal neck clipping, aneurysm neck reconstructions, aneurysmotomy, and aneurysmectomy. This patient had a previously coiled, unruptured, superiorly projecting giant anterior communicating artery (ACom) aneurysm, eccentric toward the left, for which surgical intervention was undertaken. A left orbitozygomatic craniotomy was performed, and a temporary clip was applied to the bilateral proximal A1 segments. Aneurysmotomy was then performed with internal debulking of the aneurysmal thrombus. Aneurysmectomy and removal of the coil mass were performed. Next, the aneurysm neck was reconstructed using multiple surgical clips. After anticipated aneurysm neck reconstruction, indocyanine green (ICG) angiography demonstrated a lack of flow in the ipsilateral A2. The ACom was then transected along the aneurysm neck, and an end-to-end anastomosis of the distal A1 and proximal A2 was performed. Repeat ICG angiography demonstrated patency of the A1-A2 anastomosis. The patient gave informed consent for surgery and video recording. Institutional review board approval was deemed unnecessary. Used with permission from Barrow Neurological Institute, Phoenix, Arizona.