bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD109 mediates tumorigenicity and cancer aggressiveness via regulation of EGFR and STAT3 signalling in cervical squamous cell carcinoma
Authors: Mo Xue-Tang , Leung Thomas Ho-Yin , Tang Hermit Wai-Man , Siu Michelle Kwan-Yee , Wan Peter Kok-Ting , Chan Karen Kar-Loen , Cheung Annie Nga-Yin , Ngan Hextan Yuen-Sheung 
Published: June 2020

Abstract
Background
CD109 was involved in the tumorigenesis and progression of various cancers via TGF-β1 signalling and STAT3 activation. As CD109 is strongly expressed in cervical squamous cell carcinoma, this study was conducted to investigate its functional characteristics in cervical cancer.

Methods
CD109 expression was examined by immunohistochemistry (IHC) with cervical tissue microarray. The effects of CD109 expression were examined on migration, cell proliferation, spheroid formation and soft-agar colony-formation assay. Meanwhile, cervical cancer cell lines with high CD109 expression were chosen for the functional study using siRNA knockdown and CRISPR/Cas9 knockout.

Results
IHC demonstrated an upregulation of CD109 in the cell membrane of cervical squamous cell carcinoma. CD109( + ) cells isolated by flow-cytometric sorting displayed enhanced migration, cell proliferation, sphere-forming and anchorage-independent cell growth ability. In contrast, silencing of CD109 expression could reverse the in vitro and in vivo tumorigenic and aggressive properties. Furthermore, CD109 induced EGFR-mediated STAT3 phosphorylation known to be responsible for cell migration, proliferation and maintenance of CSC phenotype.

Conclusion
Abundant CD109( + ) populations in cervical cancer cells potentially contributed to carcinogenesis and aggressiveness, whereas silencing of CD109 expression could reverse those properties. CD109 mediates cervical tumorigenicity and aggressiveness via CD109/EGFR/STAT3 signalling.
The circadian clock gene Bmal1 facilitates cisplatin-induced renal injury and hepatization
Authors: Zha Min , Tian Ting , Xu Weilong , Liu Su , Jia Jia , Wang Lijuan , Yan Qianhua , Li Nan , Yu Jiangyi , Huang Liji 
Published: June 2020

AbstractCisplatin is one of the most potent chemotherapy drugs to treat cancers, but its clinical application remains limited due to severe nephrotoxicity. Several approaches have been developed to minimize such side effects, notably including chronotherapy, a well-known strategy based on the circadian clock. However, the component of the circadian clock machinery that particularly responses to the cisplatin stimulation remains unknown, including its functions in cisplatin-induced renal injury. In our present study, we demonstrated that Bmal1, as a key clock gene, was induced by the cisplatin stimulation in the mouse kidney and cultured human HK-2 renal cells. Gain- and loss-of-function studies indicated that Bmal1 facilitated cisplatin-induced renal injury both in vivo and in vitro, by aggravating the cell apoptotic process. More importantly, RNA-seq analysis revealed that Bmal1 triggered the expression of hallmark genes involved in renal hepatization, a critical event accompanied by the injury. At the molecular level, Bmal1 activated the transcription of hepatization-associated genes through direct recruitment to the E-box motifs of their promoters. Our findings suggest that Bmal1, a pivotal mediator induced renal injury in response to cisplatin treatment, and the therapeutic intervention targeting Bmal1 in the kidney may be a promising strategy to minimize the toxic side-effects of cisplatin in its clinical applications.
Shedding light on dark genes: enhanced targeted resequencing by optimizing the combination of enrichment technology and DNA fragment length
Authors: Iadarola Barbara , Xumerle Luciano , Lavezzari Denise , Paterno Marta , Marcolungo Luca , Beltrami Cristina , Fortunati Elisabetta , Mei Davide , Vetro Annalisa , Guerrini Renzo , Parrini Elena , Rossato Marzia , Delledonne Massimo 
Published: June 2020

AbstractThe exome contains many obscure regions difficult to explore with current short-read sequencing methods. Repetitious genomic regions prevent the unique alignment of reads, which is essential for the identification of clinically-relevant genetic variants. Long-read technologies attempt to resolve multiple-mapping regions, but they still produce many sequencing errors. Thus, a new approach is required to enlighten the obscure regions of the genome and rescue variants that would be otherwise neglected. This work aims to improve the alignment of multiple-mapping reads through the extension of the standard DNA fragment size. As Illumina can sequence fragments up to 550 bp, we tested different DNA fragment lengths using four major commercial WES platforms and found that longer DNA fragments achieved a higher genotypability. This metric, which indicates base calling calculated by combining depth of coverage with the confidence of read alignment, increased from hundreds to thousands of genes, including several associated with clinical phenotypes. While depth of coverage has been considered crucial for the assessment of WES performance, we demonstrated that genotypability has a greater impact in revealing obscure regions, with ~1% increase in variant calling in respect to shorter DNA fragments. Results confirmed that this approach enlightened many regions previously not explored.
Reciprocal Benefits of Engaging Undergraduate Researchers in Conducting a Systematic Literature Review
Authors: Look Rachael , Shoemaker Hannah , Hoepner Jerry Kevin , Blake Margaret Lehman 
Published: June 2020

Purpose
This project used a reflective practice framework to examine outcomes of an undergraduate student–faculty collaborative research project. The project examined benefits of undergraduate contributions to a large-scale systematic review and explore benefits to the students.

Conclusions
Throughout a large literature search process, two student researchers documented their reflections, including field notes and written reflections about their personal learning. Students completed searches of articles about speech and language impairments associated with right hemisphere damage. In the process, a repository of articles was created for further examination by a writing committee of the Academy of Neurologic Communication Disorders and Sciences. Student accuracy in identifying potential articles that met inclusion criteria was measured by comparing student inclusions to those identified by committee members. Authors examined student reflection outcomes in the context of existing research about student–faculty collaborative research outcomes. Broad-based student outcomes included improvements in understanding the research process, collaboration skills, and confidence, along with opportunities to network/disseminate findings, focus career interests, and foster continued interest in research. Outcomes specific to the project included an increase in topic knowledge and increased accuracy in identifying articles that met inclusion criteria. The two undergraduate student researchers were able to search and identify potential articles for the systematic review. This provided an opportunity to become more proficient in searching databases and interpreting articles. Beyond direct contributions to the systematic review project, both undergraduates reported growth as student learners, researchers, and future clinicians. The students also report ongoing interests in research and considering academic careers.
Addressing the Gap in Education for Cleft Palate: A Module Training Series for Craniofacial Assessment and Treatment
Authors: Baigorri Miriam , Crowley Catherine , Sommer Chelsea 
Published: June 2020

Purpose
A shortage of speech-language pathologists (SLPs) with expertise in cleft lip and palate (CLP) care exists in the United States and worldwide. Limited educational and clinical preparation can result in ineffective intervention for individuals with CLP. Yet, comprehensive cleft care is a requisite for patients who require such services from SLPs. It is critical that practitioners possess the necessary knowledge and skills. In response to the shortage of qualified personnel, the authors developed an online cleft palate self-study course. This clinical focus article discusses the development process and provides a description of the video tutorials. A preliminary examination of the effectiveness of the tutorials via a quasi-experimental study was conducted with a group of 25 graduate students who had not taken an undergraduate or graduate course that either exclusively or partially covered the topic of CLP.

Conclusion
Initial investigation of the tutorials via quasi-experimental assessment and feedback from consumers suggests that this resource may serve as an alternative and/or supplemental tool for the education of SLPs, parents, and allied professionals.
Bougie through Murphy’s Eye During Airway Exchange: A Near Miss
Authors: Sameera Vattipalli , Biswas Konish , Rath Girija P. 
Published: June 2020

AbstractEndotracheal tube (ETT) exchange is a common procedure performed in the operating room and intensive care unit for the purpose of changing damaged or soiled ETT with appropriate one. The exchange procedure is performed over an airway exchange catheter or ETT introducer (bougie). We report an event during ETT exchange over bougie wherein the introducer exited through Murphy’s eye. Here, we intend to re-emphasize the safety concerns pertaining to the use of bougie (with coude tip) as airway exchange conduit and enumerate the precautions to be taken during tube exchange.
Cardiac PET-CT: A Brief Review of Indications and Feasibility in the Indian Scenario
Authors: Nallapareddy Kavitha , Rayaprolu V. L. Gowthami 
Published: June 2020

AbstractCardiac positron emission tomography (PET) is a functional imaging modality with the predominant indications being an assessment of myocardial perfusion and viability using short-lived positron emitters. PET procedures are used for perfusion assessment only when single-photon emission computed tomography is equivocal. Perfusion agents are ultra short-lived and have limited availability in our country, that is, only at centers with onsite cyclotron. Viability assessment with fluorodeoxyglucose (FDG) is widely performed in many centers across the country due to the availability of FDG. The other important indication is in the detection of inflammatory myocarditis, the most common being cardiac sarcoidosis done with special dietary instructions and FDG as a tracer. This indication is widely used for early detection of myocardial inflammation which would be reversible if treated early. It is also used in the assessment of treatment response. Newer tracer 68 Ga Dotapeptide is also used in this application.
Prosody transfer failure despite cross-language similarities
Authors: Liu Di 
Published: June 2020

AbstractThis study investigates how English and Mandarin speakers (1) use pitch resets and pauses to signal discourse boundaries, and (2) use pitch, duration, and intensity to indicate contrastive stress in their L1s. This study also explores how Mandarin-speaking English learners use prosodic features in L1 Mandarin and L2 English. Linear mixed-effects models showed that Mandarin and English share similarities in the forms and functions of prosody. However, Mandarin-speaking English learners did not transfer prosody usage to L2 English despite these similarities. These findings suggest that L2 prosody learning is not a typological transfer between two static language systems. Rather, it involves the interaction between two complex and dynamic prosody systems, each with its own mapping between prosodic forms and functions. Prosody teaching, therefore, should take into account the dynamic nature of prosody and compare L1 and L2 prosody in forms, meanings, and functions.
Dimensions of bilingualism promoting cognitive control
Authors: Khodos Iryna , Moskovsky Christo 
Published: June 2020

Abstract
The study investigated the capacity of language experiences to predict cognitive performance of bilingual adults,
with a special focus on participants’ proactive (mixing costs) and reactive (switching costs) control processes. Using a Language
and Social Background Questionnaire, demographic and language data were collected from a linguistically diverse group of 60
bilingual adults residing in Australia. The participants were then tested on a non-verbal switching task. The results of multiple
regressions revealed that two of the language variables being examined accounted for the variance in the mixing and switching
costs. In particular, reduced mixing costs were related to the use of two languages in a dual-language context and earlier onset
age of active bilingualism; reduced switching costs were linked to a dual-language context only. These findings reveal that
bilingual experiences contribute to shaping proactive and reactive control processes across cognitive domains.
Introduction
Authors: Mardale Alexandru , Montrul Silvina 
Published: June 2020

Abstract
This volume brings together a selection of papers that were
presented at the international workshop on the acquisition of Differential
Object Marking (DOM) organized by Alexandru Mardale (INaLCO, SeDyL) in Paris
on December 10, 2016 as part of the Unity and diversity in
Differential Object Marking research project funded by the
Fédération Typologie et Universaux Linguistiques of the CNRS. Other papers
in the volume were comissioned by Silvina Montrul (University of Illinois at
Urbana Champaign) as part of her collaborative research projects on DOM in
language acquisition. Taking a crosslinguistic perspective, the present
volume includes 13 chapters on the monolingual and bilingual acquisition of
DOM in a number of typologically unrelated languages (Basque, Estonian,
Hindi, Korean, Persian, Romanian, Spanish, Turkish), from different
theoretical and acquisition perspectives, and using different methodologies.
The new and original empirical data from diverse acquisition situations
presented in this collection contribute to advance our understanding of the
factors that characterize DOM in diverse languages and to test and evaluate
the explanatory power of available theoretical analyses of DOM and of
language acquisition.
Acquisition of symmetrical and asymmetrical Differential Object Marking
in Estonian
Authors: Vihman Virve-Anneli , Theakston Anna , Lieven Elena 
Published: June 2020

Abstract
We compared the acquisition of symmetrical and asymmetrical
Differential Object Marking (DOM) within Estonian, which employs symmetrical
DOM (alternation between overtly case-marked objects) with asymmetrical
subsystems (alternation between marked and unmarked objects) for
imperatives, impersonal voice constructions and plural objects. This
difference in marking symmetry is linked to differences in form frequency
and morphological complexity, with both factors affecting language
acquisition. Through a detailed corpus analysis, we found that marking
symmetry has an effect on one child’s DOM usage at ages 2;0 and 3;0. The
child uses more marked objects overall and makes more errors of commission
than omission in asymmetrical contexts, using case-marked objects in place
of unmarked ones. Morphological complexity and frequency of form-function
pairings were found to affect acquisition.
Differential Object Marking in simultaneous Hungarian-Romanian
bilinguals
Authors: Avram Larisa , Tomescu Veronica 
Published: June 2020

Abstract
This study charts the acquisition of Differential Object Marking
(DOM) in Romanian by Hungarian-Romanian simultaneous bilinguals (2L1). The
main finding is that these 2L1 children acquire the properties of the DOM
system similarly to monolinguals, with only one difference deriving from
descriptive DPs, with which marking is constrained by discourse-pragmatics.
During the early stages, the 2L1 children inconsistently mark these DPs in
comparison with age-matched monolinguals. Data from ‘frog story’ narratives
of 4-year-old bilinguals reveal an increase in DOM use with these DPs. The
results show that the vulnerability is selective and that it is overcome
early. We explain our findings with reference to the syntax of DOM in
Romanian and positive cross-linguistic influence from Hungarian.
The acquisition of Differential Object Marking in Basque as a
sociolinguistic variable
Authors: Rodríguez-Ordóñez Itxaso 
Published: June 2020

Abstract
Following variationist approaches to second language acquisition,
this study examines the variable use of Differential Object Marking (DOM) in
Basque as a second language and tests whether attitudes towards Basque DOM
plays a role in its use. The spontaneous speech of 15 advanced learners and
12 intermediate learners were compared to the speech of 24 Basque-dominant bilinguals. Attitude data was gathered by means of a matched-guise
experiment in which participants rated a number of guises with DOM based on
two traits (Basqueness and correctness).
Results indicated that the learners’ differences in constraining the use of
Basque DOM were affected by their attitudes towards DOM, providing further
evidence that social meaning plays a role in acquisition process of L2
grammars.
On the acceptability of the Spanish DOM among Romanian-Spanish
bilinguals
Authors: López Otero Julio César 
Published: June 2020

Abstract
Spanish and Romanian have Differential Object Marking (DOM)
systems conditioned by similar but not the same features. This study
examined whether Romanian-speaking highly proficient L2 users of Spanish
show signs of crosslinguistic influence from Romanian in their acquisition
of the distribution of DOM in Spanish. A group of Spanish-speaking
monolinguals and a group of Romanian-Spanish bilinguals who acquired Spanish
naturalistically completed an Acceptability Judgment Task that investigated
their receptive grammatical knowledge of the DOM in Spanish and, for the
bilinguals, Romanian. The results are discussed within the frame of the
Feature Reassembly Hypothesis and the Activation Approach.
Verbal lexical frequency and DOM in heritage speakers of Spanish
Authors: Hur Esther 
Published: June 2020

Abstract
This chapter examines the effect of lexical frequency of the verb
on heritage speakers’ (HS) production of Differential Object Marking (DOM)
in Spanish. Specifically, it examines whether HS are more likely to use DOM
with animate direct objects that are the complement of high frequent telic
verbs, as opposed to low-frequency telic verbs. Thirty HSs of different
levels (intermediate and advanced) and 15 Spanish-dominant controls (SDC)
completed an elicited production task (EPT). Results revealed that
intermediate heritage speakers produced more DOM with high-frequency verbs
but such an effect was not found in the advanced group. In explaining our
data, we follow the activation approach that argues that the variability of
DOM among HS is due to how frequently the HS activates the functional
features (FF) of the heritage language (HL) for production purposes.
The processing of Differential Object Marking by heritage speakers of
Spanish
Authors: Arechabaleta Regulez Begoña 
Published: June 2020

Abstract
This study examines the online processing of Differential Object
Marking by heritage speakers of Spanish living in the U.S. Previous research
has found that even heritage speakers with high proficiency in Spanish omit
DOM with animate objects in oral production and find omitted DOM acceptable
in judgment tasks. Therefore, this study investigated whether heritage
speakers’ acceptability of DOM omission is reflected in their language
processing. Twenty monolingual-raised native speakers and 20 heritage
speakers participated in an eye-tracking reading experiment. Sentences
varied in DOM marking (use vs. omission) and word order (SVO vs. VSO).
Overall, reading time data indicated that heritage speakers behaved more
similarly to monolingual-raised native speakers than predicted. The
potential effects of language variation and language attrition are
discussed.
Risk from low blood pressure in frail older adults: diastolic pressure and pulse pressure are important
Authors: Spence J David 
Published: June 2020

Abstract
Blood pressure measurement with a cuff in patients with stiff arteries can be misleading, with false elevation of the diastolic pressure. Coronary flow, and most of the cerebral blood flow occur during diastole. There is a marked diastolic J curve in patients with diastolic pressure &lt;60 mmHg and pulse pressure &gt;60 mmHg. Aiming for a systolic target of 120 mmHg is not safe in some frail older patients.
Skin Exposure to Acrylates in Nail Salons
Authors: Kalenge Sheila , Kirkham Tracy L , Nguyen Linh V , Holness D Linn , Arrandale Victoria H 
Published: June 2020

Abstract

Objectives
The nail salon industry has seen significant growth in recent years. Nail technicians provide manicures, pedicures and apply artificial nails; tasks that expose them to chemicals, including acrylates that are known skin and respiratory sensitizers. This paper reports on potential skin exposure to acrylates among nail technicians in Toronto, Canada.

Methods
Hand skin wipes were collected after (i) the application of artificial nails, (ii) the application of ultraviolet cured gel nail polish, and (iii) deliberate contact with nail polish. Surface wipes were collected from work areas and surfaces contaminated with known products. Wipe samples were analysed for eight acrylates: methyl acrylate (MA), ethyl acrylate (EA), methyl methacrylate (MMA), ethyl methacrylate (EMA), 2-hydroxyethyl acrylate (HEA), 2-hydroxyethyl methacrylate (HEMA), 2-hydroxypropyl methacrylate (HPMA), and ethylene glycol dimethacrylate (EGDMA) by gas chromatography with mass spectrometry.

Results
Thirty-five wipe samples were collected. No acrylates were detected in skin samples (n = 8) or work surfaces (n = 6). However, MMA, EMA, EA, HEMA, and HPMA were detected in the samples of surfaces contaminated with known products. MMA was detected in 28% of products tested. HEMA and HPMA were detected where deliberate contact with nail polish occurred.

Conclusions
These results confirm that acrylates are present in nail products. However, no acrylates were detected in skin wipes. MMA, a chemical prohibited for use in cosmetics in Canada, was detected in nail polishes. Nail technicians should continue to employ safe work practices that reduce the probability of skin contact with acrylates and other chemicals.
Altmetrics in Plastic Surgery Journals: Does It Correlate With Citation Count?
Authors: Asaad Malke , Howell Skyler M , Rajesh Aashish , Meaike Jesse , Tran Nho V 
Published: June 2020

Abstract

Background
Altmetrics (alternative metrics) have become one of the most commonly utilized metrics to track the impact of research articles across electronic and social media platforms.

Objectives
The goal of this study was to identify whether the Altmetric Attention Score (AAS) is a good proxy for citation counts and whether it can be employed as an accurate measure to complement the current gold standard.

Methods
The authors conducted a citation analysis of all articles published in 6 plastic surgery journals during the 2016 calendar year. Citation counts and AAS were abstracted and analyzed.

Results
A total of 1420 articles were identified. The mean AAS was 11 and the median AAS was 1. The journal with the highest mean AAS was Aesthetic Surgery Journal (31), followed by Plastic and Reconstructive Surgery (19). A weak positive correlation was identified (r = 0.33, P &lt; .0001) between AAS and citations. Articles in the top 1% in terms of citation counts showed strong positive correlation between AAS and citation counts (r = 0.64, P = .01). On the contrary, articles in the top 1% of AAS had no significant correlation with citation counts (r = −0.31, P = .29).

Conclusions
Overall correlation between citations and AAS was weak, and therefor AAS may not be an accurate early predictor of future citations. The 2 metrics seem to measure different aspects of the impact of scholarly work and should be utilized in tandem for determining the reach of a scientific article.