bluuebunny/crossref_metadata_2025_split huggingface dataset:
NORAD orchestrates endometrial cancer progression by sequestering FUBP1 nuclear localization to promote cell apoptosis
Authors: Han Tong , Wu Yukang , Hu Xiang , Chen Yaqi , Jia Wenwen , He Qizhi , Bian Yiding , Wang Mengfei , Guo Xudong , Kang Jiuhong , Wan Xiaoping 
Published: June 2020

AbstractLong noncoding RNAs (lncRNAs) are emerging as critical regulators in tumor initiation and progression. However, the biological mechanisms and potential clinical application of lncRNA NORAD in endometrial cancer (EC) remain unknown. Herein, we identified NORAD underwent promoter hypermethylation-associated downregulation in EC. Epigenetic inactivation of NORAD was correlated with EC progression (FIGO stage) and poor outcome. Overexpression of NORAD significantly inhibited cell growth and promoted apoptosis in EC cells. Mechanistic studies revealed that multiple regions of NORAD served as a platform for binding with the central domain of anti-apoptotic factor FUBP1. Our findings further indicated that the NORAD/FUBP1 interaction attenuated FUBP1 nuclear localization and thus impaired the occupancies of FUBP1 on its target pro-apoptotic gene promoters, resulting in apoptosis induction in EC. Moreover, knockdown of NORAD promoted tumor growth in the xenograft mice model. While, introduction of NORAD-4 fragment, which bound with FUBP1, successfully reversed tumor growth and apoptosis inhibition mediated by NORAD knockdown in vivo. Our findings provide mechanistic insight into the critical roles of NORAD as a tumor suppressor in EC progression. NORAD could possibly serve as a novel prognostic biomarker and provide the rationale for EC therapy.
Associations of sense of coherence and self-efficacy with health status and disease severity in COPD
Authors: Tsiligianni Ioanna , Sifaki-Pistolla Dimitra , Gergianaki Irini , Kampouraki Maria , Papadokostakis Polyvios , Poulonirakis Ioannis , Gialamas Ioannis , Bempi Vasiliki , Ierodiakonou Despo 
Published: June 2020

AbstractSense of coherence and self-efficacy has been found to affect health-related quality of life in chronic diseases. However, research on respiratory diseases is limited. Here we report findings on quality of life (QoL) of COPD patients and the associations with coherence and self-efficacy. This study consists of the Greek national branch of the UNLOCK study, with a sample of 257 COPD patients. Coherence and self-efficacy are positively inter-correlated (Pearson rho = 0.590, p < 0.001). They are negatively correlated with the quality of life (CAT) [Pearson rho: coherence = −0.29, p < 0.001; self-efficacy = −0.29, p < 0.001) and mMRC (coherence = −0.37, p < 0.001; self-efficacy rho = −0.32, p < 0.001)]. Coherence is inversely associated with (Global Initiative for Chronic Obstructive Lung Disease) GOLD 2018—CAT and GOLD 2018—mMRC classification and “having at least one exacerbation in the past year”. Findings are stressing the need for their incorporation in primary health care and COPD guidance as it maybe that enhancing coherence and self-efficacy will improve QoL.
Draft genome of the European medicinal leech Hirudo medicinalis (Annelida, Clitellata, Hirudiniformes) with emphasis on anticoagulants
Authors: Kvist Sebastian , Manzano-Marín Alejandro , de Carle Danielle , Trontelj Peter , Siddall Mark E. 
Published: June 2020

AbstractThe European medicinal leech has been used for medicinal purposes for millennia, and continues to be used today in modern hospital settings. Its utility is granted by the extremely potent anticoagulation factors that the leech secretes into the incision wound during feeding and, although a handful of studies have targeted certain anticoagulants, the full range of anticoagulation factors expressed by this species remains unknown. Here, we present the first draft genome of the European medicinal leech, Hirudo medicinalis, and estimate that we have sequenced between 79–94% of the full genome. Leveraging these data, we searched for anticoagulation factors across the genome of H. medicinalis. Following orthology determination through a series of BLAST searches, as well as phylogenetic analyses, we estimate that fully 15 different known anticoagulation factors are utilized by the species, and that 17 other proteins that have been linked to antihemostasis are also present in the genome. We underscore the utility of the draft genome for comparative studies of leeches and discuss our results in an evolutionary context.
SLC25A11 serves as a novel prognostic biomarker in liver cancer
Authors: Pan Guoqiang , Wang Ruobing , Jia Shengnan , Li Yanqing , Jiao Yan , Liu Nan 
Published: June 2020

AbstractLiver cancer is a disease with high mortality; it is often diagnosed at intermediate and advanced stages and has a high recurrence rate. ROS restriction and adequate energy supply play significant roles in liver cancer. SLC25A11, a member of the malate-aspartate shuttle (MAS), regulates electroneutral exchange between 2-oxoglutarate and other dicarboxylates. It transports glutathione (GSH) from the cytoplasm into mitochondria to maintain GSH levels to limit ROS production. Moreover, SLC25A11 is essential for ATP generation in cancers as it regulates NADH transportation from the cytoplasm to mitochondria. The purpose of this research was to investigate the prognostic value of SLC25A11 in liver cancer. The Cancer Genome Atlas database was used to analyze the levels of SLC25A11 in liver cancer. Fisher’s exact and chi-square tests were used to evaluate the relationship between SLC25A11 expression and clinical characteristics. Finally, we explored the value of SLC25A11 in prognosis by Cox analysis and Kaplan-Meier curves. Our results revealed that SLC25A11 was downregulated in liver cancer compared to normal controls. Low expression of SLC25A11 was associated with clinical stage, vital status, histologic grade, overall survival (OS) and relapse-free survival (RFS). Liver cancer patients with low SLC25A11 expression had shorter OS and RFS than patients with high SLC25A11 expression. Multivariate analysis showed that the expression of SLC25A11 was an independent predictor of RFS and OS. In conclusion, this study identified that SLC25A11 serves as a new prognostic marker for liver cancer.
Unsuspected Paraganglioma—Infratemporal Fossa
Authors: Sultana J Nikhat , Gurrala Shekhar R. , Kommula Lakshman K. , Boola Gnana R. 
Published: June 2020

AbstractParaganglioma of the infratemporal fossa is exceedingly rare, with no more than a handful of documented cases. Undiagnosed paraganglioma poses a great challenge. We present a 39-year-old hypertensive female, who presented with giddiness and headache for 3 months and four episodes of syncope over 3 months. CT and MRI revealed an enhancing infratemporal region space-occupying mass close proximity to petrous carotid artery. Intraoperatively, the patient had hemodynamic instability (tachycardia and hypertension) when the mass was surgically manipulated. Diagnosis of paraganglioma was suspected, based on hemodynamic instability, which was later confirmed by histopathology. Undiagnosed paraganglioma pose a great challenge to the anesthetic management. Low-threshold of suspicion has to be there to avoid significant morbidity and mortality.
Test the Best: Classification Accuracies of Four Cognitive Rating Scales for Parkinson’s Disease Mild Cognitive Impairment
Authors: Mazancova Adela Fendrych , Růžička Evžen , Jech Robert , Bezdicek Ondrej 
Published: June 2020

Abstract

Objective
A progressive cognitive impairment is one of the frequent non-motor symptoms during Parkinson’s disease (PD) course. A short and valid screening tool is needed to detect an incipient cognitive deficit at the mild cognitive impairment stage in Parkinson’s disease (PD–MCI).

Method
The present study aims to evaluate the classification accuracies of four cognitive screenings: Montreal Cognitive Assessment (MoCA), Mattis Dementia Rating Scale second edition (DRS–2), Mini-Mental State Examination (MMSE) and Frontal Assessment Battery (FAB) in a cohort of PD patients (PD–MCI, n = 46; and Parkinson’s disease with normal cognition, PD-NC, n = 95) and Controls (n = 66). All subjects underwent a standard neuropsychological battery as recommended by the International Parkinson and Movement Disorder Society and underwent all four screening tools.

Results
In the detection of PD-MCI versus PD-NC, the MoCA showed a sensitivity of 84% and a specificity of 66% with a screening cutoff score at ≤25 points. The MoCA’s AUC was 86% (95% CI 78.7–93.1). In the detection of PD-MCI versus Controls, the FAB displayed 84% sensitivity and 79% specificity with a cutoff ≤16 points, to screen. The FAB’s AUC was 87% (79.0–95.0).

Conclusions
Our results show that the MoCA is the most discriminative tool for screening MCI in the PD population.
ProbeRating: a recommender system to infer binding profiles for nucleic acid-binding proteins
Authors: Yang Shu , Liu Xiaoxi , Ng Raymond T 
Published: June 2020

Abstract

Motivation
The interaction between proteins and nucleic acids plays a crucial role in gene regulation and cell function. Determining the binding preferences of nucleic acid-binding proteins (NBPs), namely RNA-binding proteins (RBPs) and transcription factors (TFs), is the key to decipher the protein–nucleic acids interaction code. Today, available NBP binding data from in vivo or in vitro experiments are still limited, which leaves a large portion of NBPs uncovered. Unfortunately, existing computational methods that model the NBP binding preferences are mostly protein specific: they need the experimental data for a specific protein in interest, and thus only focus on experimentally characterized NBPs. The binding preferences of experimentally unexplored NBPs remain largely unknown.

Results
Here, we introduce ProbeRating, a nucleic acid recommender system that utilizes techniques from deep learning and word embeddings of natural language processing. ProbeRating is developed to predict binding profiles for unexplored or poorly studied NBPs by exploiting their homologs NBPs which currently have available binding data. Requiring only sequence information as input, ProbeRating adapts FastText from Facebook AI Research to extract biological features. It then builds a neural network-based recommender system. We evaluate the performance of ProbeRating on two different tasks: one for RBP and one for TF. As a result, ProbeRating outperforms previous methods on both tasks. The results show that ProbeRating can be a useful tool to study the binding mechanism for the many NBPs that lack direct experimental evidence. and implementation

Availability and implementation
The source code is freely available at &lt;https://github.com/syang11/ProbeRating&gt;.

Supplementary information
Supplementary data are available at Bioinformatics online.
Allografts and xenografts for right ventricular outflow tract reconstruction in Ross patients
Authors: Sharifulin Ravil , Bogachev-Prokophiev Alexander , Demin Igor , Afanasyev Alexander , Ovcharov Mikhail , Pivkin Alexey , Sapegin Andrey , Zhuravleva Irina , Karaskov Alexander 
Published: June 2020

Abstract

OBJECTIVES

Pulmonary allografts (AG) are the gold standard for right ventricular outflow tract (RVOT) reconstruction during the Ross procedure. However, there is limited availability of AG in some countries, and the use of alternative grafts for RVOT reconstruction remains controversial. This study aimed to compare the rates of freedom from RVOT graft dysfunction for AG and diepoxide-treated pericardial xenografts (DPXG).

METHODS
Between 1998 and 2015, 793 adult patients underwent the Ross procedure in our centre. Using propensity score matching, the clinical outcomes and echocardiographic results of AG and DPXG were compared.

RESULTS
Propensity score matching resulted in 2 groups (AG and DPXG) of 122 patients each. No difference was found in early mortality (2.5%) in both groups. The freedom from RVOT graft dysfunction curves were comparable between the AG and DPXG groups (P = 0.186) and the 8-year rates of freedom from graft dysfunction were 91.8% and 82.2%, respectively. The survival rates at 8 years were 90.5% and 90.1%, and the rates of freedom from RVOT reintervention at 8 years were 100% and 96.8% for the AG and DPXG groups, respectively. At discharge and follow-up, transprosthetic gradients were significantly higher in the DPXG group. The rate of the RVOT gradient progression was also higher in the DPXG group than in the AG group (1.80 ± 0.06 vs 1.39 ± 0.04 mmHg/year, P &lt; 0.001).

CONCLUSIONS
There was no difference in freedom from RVOT graft dysfunction by 8 years when using AG and DPXG in adult Ross patients, nor in survival and freedom from RVOT conduit reintervention. Long-term results need further evaluation.
Microbiome approaches provide the key to biologically control postharvest pathogens and storability of fruits and vegetables
Authors: Kusstatscher Peter , Cernava Tomislav , Abdelfattah Ahmed , Gokul Jarishma , Korsten Lise , Berg Gabriele 
Published: June 2020

ABSTRACT
Microbes play an important role in plants and interact closely with their host starting from sprouting seeds, continuing during growth and after harvest. The discovery of their importance for plant and postharvest health initiated a biotechnological development of various antagonistic bacteria and fungi for disease control. Nevertheless, their application often showed inconsistent effects. Recently, high-throughput sequencing-based techniques including advanced microscopy reveal fruits and vegetables as holobionts. At harvest, all fruits and vegetables harbor a highly abundant and specific microbiota including beneficial, pathogenic and spoilage microorganisms. Especially, a high microbial diversity and resilient microbial networks were shown to be linked to fruit and vegetable health, while diseased products showed severe dysbiosis. Field and postharvest handling of fruits and vegetables was shown to affect the indigenous microbiome and therefore has a substantial impact on the storability of fruits and vegetables. Microbiome tracking can be implemented as a new tool to evaluate and assess all postharvest processes and contribute to fruit and vegetable health. Here, we summarize current research advancements in the emerging field of postharvest microbiomes and elaborate its importance. The generated knowledge provides profound insights into postharvest microbiome dynamics and sets a new basis for targeted, microbiome-driven and sustainable control strategies.
The Role of Polygenic Score and Cognitive Activity in Cognitive Functioning Among Older Adults
Authors: Shin Su Hyun , Park Soohyun , Wright Cheryl , D’astous Valerie Anne , Kim Giyeon 
Published: June 2020

Abstract

Background and Objectives
This study explored whether the intensity of cognitive activities could moderate the relationship between a genetic predisposition for developing Alzheimer’s disease (AD) and cognitive functioning among older adults in the United States. Furthermore, we examined whether the same moderating effects were dependent on different measures of cognition.

Research Design and Methods
We used a data set from the 2000–2014 waves of the Health and Retirement Study and the Consumption and Activities Mail Survey. Our sample included 3,793 individuals aged 50 or older. We used the polygenic score (PGS) for AD as a genetic trait for cognitive functioning. Reading, listening to music, using a computer, playing cards/games/solving puzzles, singing/playing musical instruments, and creating art and crafts were included as cognitive activities, and TV viewing as passive activities. We used total cognition, fluid intelligence, and crystallized intelligence as proxies for cognitive functioning. Growth-curve models were conducted.

Results
After controlling for covariates, we found that reading books, using a computer, and playing cards/games/solving puzzles had a positive effect on cognitive functioning. An additional hour spent reading books moderated the negative effect of AD PGS on cognition. The measure of fluid, when compared with crystallized intelligence, appeared to drive these results.

Discussion and Implications
Reading could be a protective factor against cognitive decline among older adults who are genetically predisposed to developing AD. Implications for individuals, caregivers, clinicians, and policymakers are suggested. Furthermore, the onset of AD in those at greater genetic risk may be delayed with this intervention.
The UPC without the UK: Consequences and Alternatives
Authors: Tilmann Winfried 
Published: June 2020

Abstract
The UK has announced its withdrawal from the UPCA. In order to effectively leave the group of CMS it would have to notify its departure from the UPCA and the two Regulations to the EU Council and from the Protocol on provisional application to the Council of Europe. The UPCA-CMS on the continent should quickly pick up preparatory work after a new ratification law has been enacted in Germany consequential to the decision of the German Constitutional Court. The alternative of merging the UPC with a new specialised court within the Court of the CJEU has no realistic prospect of succeeding because ratification by all EU Member States is necessary.
Geometric curve flows in low dimensional Cayley–Klein geometries
Authors: Benson Joseph , Valiquette Francis 
Published: June 2020

Abstract
Using the method of equivariant moving frames, we derive the evolution equations for the curvature invariants of arc-length parametrized curves under arc-length preserving geometric flows in two-, three- and four-dimensional Cayley–Klein geometries. In two and three dimensions, we obtain recursion operators, which show that the curvature evolution equations obtained are completely integrable.
The impact of metabolites derived from the gut microbiota on immune regulation and diseases
Authors: Ohno Hiroshi 
Published: June 2020

Abstract
Abstract
The gut microbiota strongly impacts the physiology and pathology in the host. To understand the complex interactions between host and gut microbiota, an ‘integrated omics’ approach has been employed, where exhaustive analyses for the different layers of cellular functions, such as epigenomics, transcriptomics and metabolomics, in addition to metagenomics, are combined. With this approach, the mechanisms whereby short-chain fatty acids (SCFAs) regulate host defense and the immune system have been elucidated. In a gnotobiotic mouse model of enterohemorrhagic Escherichia coli infection, Bifidobacterium-derived acetate can protect from infection-mediated death by changing the gene expression profile of colonic epithelial cells. It has also been shown that gut microbiota-derived butyrate enhances colonic regulatory T-cell differentiation through its epigenetic modulatory ability via histone deacetylase inhibition. SCFAs are involved in many other immunomodulatory effects as well as host pathophysiological conditions. Dysbiosis in the gut has been implicated in the pathogenesis of many diseases. Although the causal relationship of gut microbial dysbiosis and/or metabolites with pathogenesis is mostly unknown, mechanistic insights have been elucidated in some cases. Metabolism in the gut microbiota and host liver produces trimethylamine N-oxide, which is known to aggravate atherosclerosis, and a secondary bile acid deoxycholate, which reportedly induces non-alcoholic steatohepatitis-related hepatocellular carcinoma. It has been reported that secondary bile acids could also induce the differentiation of peripherally derived regulatory T cells in the gut. Further studies on the interactions between the host and gut microbiota could lead to the development of new therapeutic strategies as well as in preventive medicine.
Outdoor Programs for Veterans: Public Land Policies and Practices to Support Therapeutic Opportunities
Authors: Derrien Monika M , Cerveny Lee K , Havlick David G 
Published: June 2020

Abstract
Many veterans returning from military deployment experience stress- or trauma-related symptoms that make reintegration with civilian society difficult. Nature exposure and outdoor recreation can be important parts of alternative and complementary approaches to reduce symptoms and build on veterans’ pre-existing strengths. Multiple outdoor programs now exist for veterans; many of these occur on federal public lands and present a variety of needs, opportunities, and challenges. This paper relies on interviews (n = 36) with public land managers, program providers and participants, health professionals, and veterans to enhance understanding about outdoor programs for veterans (OPVs). We develop a typology of OPVs to help land managers understand current and potential programs, and then describe programs’ varying dimensions. We examine opportunities and challenges for land managers in their interactions with OPVs. Results inform policymakers and public officials interested in developing more effective institutional partnerships and programs that engage and serve veterans and their communities.
Study Implications: With growing scientific evidence of the benefits of nature-based therapy, nature exposure, and outdoor recreation for veterans, programming for veterans on public lands has proliferated. Public land-management agencies vary in the extent to which they have systematically organized to provide opportunities for veterans, developed partnerships to support veterans’ health, and explicitly acknowledged agency roles in serving veterans. We describe seven types of outdoor programs for veterans (OPVs) that currently serve this population: supported outdoor activity; guided outdoor activity; retreat; outdoor job training; stewardship or service; horticulture, farming or gardening; and animal-assisted therapy. Each OPV type has different needs for infrastructure, outdoor spaces, and administrative or programmatic engagement. OPVs occurring on public lands typically involve one or more partner organizations, such as commercial outfitters and guides, health providers, veterans’ associations, foundations, corporations, and research institutions. There is potential for public land-management agencies to expand their role as institutional leaders in support of veterans’ health by facilitating the use of public lands as therapeutic landscapes. By enhancing new and existing relations with OPV providers, health providers, and other government agencies, public land agencies could expand benefits to veterans and spur broader societal benefits.
Repair of a recurrent symptomatic hernia through the foramen of Morgagni: a case study and review of the literature
Authors: Ben-Yaacov Almog , Menasherov Nikolay , Bard Vyacheslav 
Published: June 2020

Abstract
The prevalence and natural history in adults of Morgagni hernias have been relatively poorly characterized. A case is presented of a 31-year-old man where the hernia recurred following a laparoscopic mesh repair. In the era of minimally invasive surgery, debate concerns whether the peritoneal sac should be excised and if the insertion of mesh is superior to primary diaphragmatic repair.