bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome Analysis Reveals Dynamic Changes of Inflammation and Stress Responses during Different Infected Stages with Burkholderia pseudomallei
Authors: Author) Chenglong Rao(Former Corresponding , Mao Chan , Xia Yupei , Zhang Meijuan , Hu Zhiqiang , Yuan Siqi , Zhang Yu , Yan Jingmin , Deng Ling , Mao Xuhu , Li Qian , Author) Yaling Liao(New Corresponding 
Published: January 2020

Abstract
Background: Burkholderia pseudomallei causes melioidosis and usually affects patients’ lungs, its persistent infection promotes the fusion of host cells, leading to the formation of multinucleated giant cells (MGCs) at the late infected stage. In this study, the global transcriptomic responses of B. pseudomallei infection of a human lung epithelial A549 cell model with different infected stages were investigated by means of microarray analysis to further elucidate the host cellular factors involved in the occurrence and development of the event. Results: A set of 35 common differential expression genes (DEGs) in EI and LI on the mRNA level applying a cut-off level of 1.5-fold change and a p-value < 0.05 were observed. Microarray data were further verified by Real-Time quantitative PCR (RT-qPCR). GO classification and pathway enrichment analysis revealed these DEGs mainly involved in inflammatory response related processes, such as cellular response to tumor necrosis factor, cellular response to lipopolysaccharide, positive regulation of NF-κB transcription factor activity. p-eIF2α, ATF4,NF-κB2(p52) and IL-1β were next selected to be validated by western bloting, which indicated B. pseudomallei could activate the eIF2α-ATF4 axis and NF-κB2 pathway in A549 cells. Conclusion: Our data shed light on the transcriptome dynamics of A549 cells which persistently infected with B. pseudomallei and suggested that the formation of MGCs may be a means for B. pseudomallei to manipulate the host's inflammation and stress response to adapt to intracellular life.
The cerebellum is associated with 2-year prognosis in patients with high-frequency migraine
Authors: Liu Hung Yu , Lee Pei Lin , Chou Kun Hsien , Lai Kuan Lin , Wang Yen Feng , Chen Shih Pin , Chen Wei Ta , Wang Shuu Jiun 
Published: January 2020

Abstract
Background: The increase of headache frequency is associated with higher headache related disability and lower quality of life in patients with migraine. However, the pathophysiology of migraine progression, persistence, or remission is elusive. The purpose of this study is to identify the brain signatures that are predictive of the long-term outcomes among patients with high-frequency migraine (HFM: 10–30 headache days/month). Methods: We prospectively enrolled patients with HFM and healthy controls and collected their baseline clinical profiles and brain-MRI data at first visit. We longitudinally followed the patients and determined their outcomes at 2-year follow-up. Good outcome was defined as ≥ 50% reduction of baseline headache days and poor outcome was defined as reduction < 50% or frequency increase. Voxel-based morphometry was used to study gray matter volume (GMV), and structural covariance was used to investigate structural connectivity. Results: Among 56 patients with HFM, 37 had good outcome and 19 poor outcome. Compared to the healthy controls (n = 37), patients with poor outcome had decreased GMV over the left posterior cingulate gyrus, and increased GMV over the bilateral cerebellum and the right precentral gyrus. Further, patients with poor outcome had greater GMV over the right and the left cerebella compared to patients with good outcome, and the GMVs of the cerebella were correlated to 2-year headache frequencies (right: r = 0.38, P = 0.005; left: r = 0.35, P = 0.009). Structural connectivity were increased between the cerebellum and the cuneus, the calcarine cortex, and the temporal lobe, respectively, in patients with poor outcome, and was decreased between the cerebellum and the prefrontal cortex in patients with poor outcome. The structural covariance integrities between the right cerebellum and the right cuneus were correlated to 2-year headache frequencies (r = 0.36, P = 0.008). Conclusions: Structural volume and connectivity changes of the cerebellum may underlie headache persistence in patients with HFM.
Development of a tool for identifying and addressing prioritised determinants of quality improvement projects led by healthcare professionals: a mixed-methods study
Authors: Tuijl Anne van , Wollersheim Hub C. , Fluit Cornelia R.M.G. , Gurp Petra. J. van , Calsbeek Hiske 
Published: January 2020

Abstract
Background: Several frameworks have been developed to identify essential determinants for healthcare improvement. These frameworks aim to be comprehensive, leading to the creation of long lists of non-prioritised determinants. Furthermore, most existing frameworks do not describe the methods or actions used to identify and address the determinants, limiting their practical value. The aim of this study is to describe the development of a tool with prioritised facilitators and barriers for healthcare improvement projects led by healthcare professionals, supplemented with methods to identify and address each determinant. Methods: A mixed-methods study design was used to develop the tool. First, a cross-sectional study was performed, using an online survey to ask healthcare professionals about the determinants they experienced during the performance of their quality improvement project. A priority score was calculated for every named determinant, and those with a priority score ≥ 20 were incorporated into the tool. Semi-structured interviews with implementation experts determined: 1) how healthcare professionals can be assisted to use our tool to analyse the determinants in their project and 2) how healthcare professionals can tackle the barriers or use the facilitators identified in their quality improvement project. Results: The 25 healthcare professionals in this study experienced 64 facilitators and 66 barriers when performing their improvement projects. Of these, 12 facilitators and nine barriers were incorporated into the tool. Sufficient support from management of the department was identified as the most important facilitator, while having limited time to perform the project was considered the biggest barrier. The interviews with 16 experts in implementation science led to various inputs for better identifying and addressing each determinant. Important themes included maintaining adequate communication with stakeholders, keeping the project at a manageable size, learning by doing and being able to influence determinants. Conclusions: This paper describes a tool for performing quality improvement projects in healthcare, with the barriers and facilitators that were considered most important by healthcare professionals. For each of these barriers and facilitators, suggestions on how to analyse and address the determinant are given. More research is needed to validate and develop the tool further.
Correlation study on gut microbiota and omentin-1 gene polymorphism in Uyghur newly diagnosed type 2 diabetes
Authors: Nuli Rebiya , Shan Jiaoyu , Zhang Bing , Li Rui , Guan Yaqun 
Published: January 2020

Abstract
Background: Type 2 diabetes (T2DM) is a top risk factor for health in China. Gut microbiota, genetic factors and lipids metabolism play important role in development of T2DM. In this study, we investigated the relationship between the gut microbiota and omentin-1 gene polymorphism to explore host gene and gut microbiota interaction in Uyghur T2DM.Methods: A total of 98 newly diagnosed Uyghur T2DM patients and 99 healthy normal controls (NC) enrolled into this study according to inclusion criteria. The total DNAs were extracted from the fecal microbiota. Abundant of the Lactobacillus genus, Bacteroides thetaiotaomicron and Clostridium in the gut microbiota were determined with 16S rDNA gene Real-time fluorescence quantitative PCR amplification. PCR-PFLP was applied to determine the genotypes of Val109Asp variant (rs2274907) in the Omentin-1 gene. And the relationship between rs2274907 and gut microbiota was assessed.Results : There were no significant differences between the genotypes of Val109Asp variant (rs2274907) of T2DM and control group. The abundances of Lactobacillus genus and Clostridium genus were lower in newly diagnosed T2DM group, compared with NC group ( P <0.05). Serum insulin, LDL-C, the abundances of Lactobacillus genus and Clostridium genus were the risk factor of T2DM. (OR=1.094 95%CI 1.014-1.180), (OR=3.868 95%CI 1.250-11.971), (OR=0.288, 95%CI 0.145-0.571), (OR=0.044, 95%CI 0.012-0.154).Conclusions: The abundance of Lactobacillus and Clostridium genus may be related to the pathogenesis of new-onset T2DM in Uyghur population, the mechanism of which needs to be further studied. The interaction relationship between the gut microbiota and omentin-1 gene polymorphism in newly diagnosed T2DM was not observed in this study.
Prevalence of ADHD symptoms and their correlations with mental health-related variables among medical student
Authors: Boonnag Chiraphat , Siritikun Sirinut , Chalanun Sirikorn , Kuntawong Pimolpun , Wongpakaran Nahathai , Wongpakaran Tinakon 
Published: January 2020

Abstract
Objectives Attention deficit hyperactivity disorder (ADHD) is a neurobehavioral development disorder contributed to social difficulties and affective problems. ADHD was evidently related to perceived stress, internet addiction and depression, leading to academic problems. However, ADHD symptoms among young medical student remains underreported. This study aimed to investigate the prevalence of ADHD and explore the association between ADHD symptoms and mental health-related conditions among medical students. Results Among 124 participants of first year medical students in Chiang Mai University, Thailand, 78 (62.9%) were female. The average age was 18.7 (SD 0.7). Thirty-one (25%) were considered as having ADHD symptoms based on the Adult ADHD Self-Report Scale screener (ASRS). The group with ADHD symptoms showed higher scores on the internet addiction test core (t = 3.27, p = .001), perceived stress scale (t = 2.94, p = .004) and Rosenberg Self-esteem Scale (t = 3.04, p = .003). No difference was found between groups regarding depression and perceived social support scores. However, the total score of ASRS appeared to significantly correlate with all these mental health- related variables. The relatively high prevalent may have contributed to invalid items of ASRS, for which a new or revised scale is needed.
The effectiveness of simulation-based training in non-physician anesthetists in Tigray region, Ethiopia
Authors: Etanaa Naod Bulti , Benwu Kore Menjie , Gebremedhin Hagos Gebregzabiher , Desta Haftom Birhane 
Published: January 2020

Abstract
Objective : In Ethiopia simulation-based anesthesia education is a new way of teaching method which started in Mekelle University as of January, 2019. Hence, the purpose of this study is to evaluate whether simulation-based training improves non-physician anesthetists’ knowledge and attitude on maternal and neonatal anesthesia cares or not. Results : A total of 50 participants were included in this study were 70 % were males and majority (66%) had a working experience of less than 5 years. Knowledge score improved significantly from 49.78% and 66.22% in pretest and posttest results respectively. The standard deviation for the knowledge score for pretest was 4.48 and for posttest it was 5.96. The posttest result was significantly improved (P< 0.001) for all knowledge questions. The respondents were asked about negative statement and a positive statement about the need to have effective closed lope communication, maternal resuscitation and neonatal resuscitation. The attitude score improved from 72.45% to 79.11% in pretest and posttest respectively. From the 9 questions the attitudinal mean score for pretest was 6.52 and posttest 7.12. The null hypothesis of equal knowledge and attitude was rejected, t (49) = -5.54, p < 0.001 and t (49) = -2.25, p < 0.03 respectively.
Maternal intensive care unit admission as an indicator of severe acute maternal morbidity: A population-based study
Authors: Godeberge Charlotte , Deneux-Tharaux Catherine , Séco Aurélien , Rossignol Mathias , Chantry Anne Alice , Bonnet Marie-Pierre 
Published: January 2020

Abstract
Background Severe acute maternal morbidity, accounting for any life-threatening complication during pregnancy or after delivery, is a major issue in maternal health. Measuring and monitoring it seems critical for assessing the quality of maternal health care. We explored the relevance of maternal ICU admission as an indicator of severe acute maternal morbidity by characterizing, among maternal ICU admissions, the profile of women with severe acute maternal morbidity and their ICU stay, according to the association with other criterion of severe acute maternal morbidity. Methods Secondary analysis of a multiregional prospective population-based study of 2,540 women with severe acute maternal morbidity according to a multicriteria definition based on national experts’ consensus and including ICU admission. Results 511 women were admitted to an ICU during or up to 42 days after pregnancy (2.8 per 1,000 deliveries; 20.1% of women with severe acute maternal morbidity); 15.5% had no other severe acute maternal morbidity criterion. Among women with severe acute maternal morbidity, on multivariable multinomial analysis and adjusting for cause, the odd of intensive care unit admission with another morbidity criterion was increased for migrant from outside of Europe or Africa (adjusted odds ratio = 2.1 [95% CI 1.3-3.4]), with multiple gestation (adjusted odds ratio =1.5 [1.0-2.2]), and intrapartum cesarean (adjusted odds ratio =1.5 [1.1-2.2]). The odd of intensive care unit admission with no other morbidity criterion was increased with pre-existing medical conditions (adjusted odds ratio =2.2 [1.2-4.0]) and cesarean before labor (adjusted odds ratio =3.0 [1.4-6.1]). Women admitted to an ICU with no other morbidity criterion had no interventions for organ support. Conclusions Among women with severe acute maternal morbidity, one in five is admitted to an ICU; 15.5% of these have no other severe acute maternal morbidity criterion and their admission appears mostly indicated for continuous monitoring. The use of ICU admission alone as a single criterion morbidity is misleading to define severe acute maternal morbidity; this criterion needs to be refined to be included in the definition of severe acute maternal morbidity. These results also challenge the current organization of acute care for women with severe maternal morbidity.
Development and usefulness of a virtual reality-based training program for promoting subjective well-being: A preliminary study
Authors: Kim Junhyung , Jung Young Hoon , Shin Yu-Bin , Kim Min-Kyeong , Eom Hyojung , Kik Eunjoo , Kim Joohan , Kim Jae-Jin 
Published: January 2020

Abstract
Background Enhancing subjective well-being is an effective way to improve mental health and virtual reality is useful as an intervention tool for cultivating well-being. This preliminary study aimed to probe the usefulness of a virtual reality-based interactive feedback program as an intervention tool for promoting subjective well-being. Methods In the experiment, 36 males participated in this program, consisting of three tasks constructed based on the theories of positive psychology: ‘Experience-based problem recognition task’, ‘Future self-based success story expression task’, and ‘Strength expression task’. Participants rated visual analog scores associated with each of the tasks' contents. The concurrent validity of task scores was evaluated by correlations with scores of the psychological scales, such as the Mental Health Continuum-Short Form, Rosenberg’s Self-Esteem Scale, Dispositional Hope Scale, and Life Orientation Test Revised. Results The total task score was positively correlated with the scores of Mental Health Continuum-Short Form emotional well-being ( r = 0.492, p < 0.001) and psychological well-being ( r = 0.501, p < 0.001), Rosenberg’s Self-Esteem Scale ( r = 0.435, p < 0.001), Dispositional Hope Scale agency dimension ( r = 0.601, p < 0.001) and pathways dimension ( r = 0.451, p < 0.001), and Life Orientation Test Revised ( r = 0.378, p < 0.05), but not with the MHC-SF social well-being scores. After controlling the effects of the other task scores, the task scores had linear relationships with certain psychological assessments. Conclusion The results showed that the total task score was significantly correlated with indicators of well-being, self-esteem, hope, and optimism. The task scores obtained from the individual tasks were closely related to the indicators, depending on the nature of the task. These findings suggest that the program contents are well associated with certain aspects of subjective well-being and thus may be available for training that improves subjective well-being through interactive feedback.
Over-expression of PTEN attenuates the inflammation of fibroblast-like synoviocytes in rheumatoid arthritis
Authors: Li Xiao-Feng , Xu Qing-Qing , Yang Man-Wen , Chen He , Yin Su-Qin , Chen Xin , Song Biao , Zhang Lei , Meng Xiao-Ming , Li Jun 
Published: January 2020

Abstract
Background: Rheumatoid arthritis (RA) is characterized by a tumor-like expansion of the synovium and the subsequent destruction of adjacent articular cartilage and bone. Recent studies have shown that phosphatase and tension homolog deleted on chromosome 10 (PTEN) might contribute to the survival of fibroblast-like synoviocytes (FLS) and the production of pro-inflammatory cytokines in RA.Methods : The expression was determined in RA and adjuvant-induced arthritis (AIA) synovial tissues by immunohistochemistry. FLSs were treatment with bpv, PTEN-RNAi or over-expression plasmid in RA and AIA. FLSs migration was assessed. The ad-PTEN was also injected into the knee of AIA in vivo. Chromatin Immunoprecipitation (ChIP) and Methylation-special PCR (MSP) assay were used to study the expression of PTEN mRNA in DNA methylation.Results : Down-regulated level of PTEN expression was observed in RA and AIA. Inhibition PTEN expression by bpv or PTEN-RNAi could promote the expression of pro-inflammatory cytokines, chemokines and migration of FLS with TNF-α in RA and AIA. Consistently, over-expression of PTEN reduced their low-expression of pro-inflammatory cytokines, chemokines and migration. Intra-articular injection of ad-PTEN in AIA knees dramatically reduced inflammatory and paw swelling in vivo. The ChIP and MSP assay has clearly detected the DNA methylation of PTEN was increased in FLS with TNF-α. Moreover, intraperitoneally injected 5-Aza in AIA also suppressed the inflammatory and paws swelling in vivo.Conclusions: Our findings suggest that over-expression PTEN attenuates the formation of pro-inflammatory cytokines, chemokines and migration of FLS, and it may be regulated by DNA methylation in the pathogenesis of RA.
Human parvoviruses affect the development and clinical course of meningitis and meningoencephalitis
Authors: Vilmane Anda , Terentjeva Anna , Tamosiunas Paulius Lukas , Suna Normunds , Suna Inga , Petraityte-Burneikiene Rasa , Murovska Modra , Rasa-Dzelzkaleja Santa , Nora-Krukle Zaiga 
Published: January 2020

Abstract
Background: Meningitis and meningoencephalitis are neurological inflammatory diseases and although routine diagnostics include testing of wide range of pathogens, still in many cases no causative agent is detected. Human parvovirus B19 (B19V), human bocaviruses 1-4 (HBoV1-4) and human parvovirus 4 (hPARV4) are members of Parvoviridae family and are associated with wide range of clinical manifestations including neurological disorders. The main aim of this study was to determine whether human parvoviruses infection markers are present among patients with meningitis/meningoencephalitis in Latvia as well as to clarify the role of these viruses on clinical course of mentioned diseases. Methods: In this study 42 cases of confirmed or unknown aetiology meningitis or meningoencephalitis were evaluated. PCR method was used for detection of B19V, HBoV1-4 and hPARV4 genomic sequences. In order to evaluate if there are some clinical differences between patient groups and in case of active parvovirus infection, nonparametric statistical tests were used. Results: Our study revealed HBoV1-4 and B19V genomic sequences in 52.38% and 16.67% of patients, respectively. Furthermore, symptoms such as the presence of a headache and its severity, fatigue, disorientation and difficulties to concentrate were significantly frequently present in patients with active parvovirus infection in comparison with parvoviruses negative patients. Conclusions: We suggest that HBoV1-4 and B19V infection should be included in the diagnostics to reduce the number of meningitis/meningoencephalitis with unknown/unexplained aetiology.
Reliability of Tibiofemoral Contact Area and Centroid Location in an Upright, Open MRI (UO-MRI)
Authors: Schmidt Andrew , Stockton David J. , Hunt Michael A. , Yung Andrew , Masri Bassam A. , Wilson David R. 
Published: January 2020

Abstract
Background: Biomechanical studies are often performed using conventional closed-bore MR, which has necessitated simulating weightbearing load on the joint. The clinical applicability of these biomechanical findings is unclear because of the limitations of simulating weightbearing. Upright, open MRI (UO-MRI) can be used to assess knee joint mechanics, in particular contact area and centroid location. However, it is not clear how reliably measurements of contact area and centroid location can be made in upright weightbearing postures. Methods: Manual segmentation of cartilage regions in contact was performed and centroids of those contact areas were automatically determined for the medial (MC) and lateral (LC) tibiofemoral compartments. To assess reliability, inter-rater, test-retest, and intra-rater reliability were determined by intra-class correlation (ICC 3,1 ), standard error of measurement (SEM), smallest detectable change with 95% confidence (SDC 95 ). Accuracy was assessed by using a high-resolution, 7T MRI as a reference and determined by measurement error (%). Results: Contact area and centroid location reliability (inter-rater, test-retest, and intra-rater) for sagittal scans in the MC demonstrated ICC 3,1 values from 0.95-0.99 and 0.98-0.99 respectively, and in the LC from 0.83-0.91 and 0.95-1.00 respectively. The smallest detectable change in contact area was 1.28% in the MC and 0.95% in the LC. Contact area and centroid location reliability for coronal scans in the MC demonstrated ICC 3,1 values from 0.90-0.98 and 0.98-1.00 respectively, and in the LC from 0.76-0.94 and 0.93-1.00 respectively. The smallest detectable change in contact area was 0.65% in the MC and 1.41% in the LC. Contact area segmentation was accurate to within 4.81% measurement error. Conclusions: Knee contact area and contact centroid location can be assessed in upright weightbearing MRI with good to excellent reliability and accuracy within 5%. The lower field strength used in upright, weightbearing MRI does not compromise the reliability of tibiofemoral contact area and centroid location measures.