bluuebunny/crossref_metadata_2025_split huggingface dataset:
The regulation and mechanism of a dual PI3K/mTOR signaling inhibitor in hemangioma vascular endothelial cells in vitro
Authors: liu wangshu , Yu Yang , Li Juan , Huang Hui , Liu Hao , Yuan Meijia , Tan Hongyang , Wang Nankai , Nie Miaojie , Hu Xuanshen , Yu Song 
Published: March 2020

Abstract
Objective To observe the influence of the dual PI3K/mTOR inhibitor NVP-BEZ235 on proliferation and apoptosis of hemangioma cells in vitro and key molecules of the PI3K/Akt/mTOR signaling pathway.Methods Hemangioma-derived endothelial cells (HeECs) were obtained by surgical resection and cultured after the explants with the trypsin-digestion method. Fourth generation cells were cultured with serum-free medium for 24 hours. Then, the intervention group cells were added to the culture medium with 0.50 μM or 1.00 μM NVP-BEZ235. Cell proliferation was detected with CCK-8 assays, apoptosis was detected by flow cytometry, and PI3K, Akt, mTOR, and p70s6k protein levels were detected by Western blots. Then, the relationship between the phenotype of hemangioma vascular endothelial cells and the four proteins was analyzed.Results the 0.50 μM and 1.00 μM NVP-BEZ235 groups were significantly lower (0.88±0.03 and 0.59±0.05, respectively) than the control group (1.10±0.02) (P<0.01). The rate of G0/G1 phase cells in the 0.50 μM and 1.00 μM NVP-BEZ235 group were higher than the control group (P<0.01). The total rates of apoptotic cells in the 0.50 μM and 1.00 μM NVP-BEZ235 groups were higher than the control group (2.77±1.23)% (P<0.01). The PI3K pathway related protein levels in the NVP-BEZ235 group were lower than control group (P<0.01).Conclusion The PI3K/Akt/mTOR signaling pathway participates in hemangioma development. NVP-BEZ235 affected hemangioma vascular endothelial cells in vitro by regulating the PI3K/Akt/mTOR signaling pathway in a dose-dependent manner.
Mini-midvastus versus medial parapatellar approach in total knee arthroplasty: difference in patient-reported outcomes measured with the Forgotten Joint Score
Authors: Lin Wei , Niu Jinghui , Dai Yike , Yang Guangmin , Li Ming , Wang Fei 
Published: March 2020

Abstract
Background: Low knee awareness after minimally invasive total knee arthroplasty (TKA) has become the ultimate target of a natural feeling knee that meet patient expectations. The objective of this research was to compare the clinical outcomes of TKA via the mini-midvastus (MMV) approach or medial parapatellar (MPP) approach and expound which approach can acquire better quality of life after surgery.Methods: From January 2015 to December 2016, a retrospective cohort study was conducted in 330 patients who underwent TKA via mini-midvastus (MMV) approach were included in MMV group. In this period, we selected 330 patients who underwent TKA via medial parapatellar (MPP) approach (MPP group) for comparison. Clinical results were assessed with visual analogue score for pain (VAS), range of motion (ROM), Knee Society Score (KSS). The forgotten joint score (FJS) was used to analyze the ability to forget the joint.Results: There were significant differences with regard to VAS, ROM and KSS score until six months after surgery between the MMV and MPP group (p < 0.05), but the differences were not found at 12 months, 24 months and 36 months after surgery. However, there were significant differences with regard to FJS score between the groups during the follow up time (p < 0.05).Conclusion: When forgetting the artificial joint after TKA is the ultimate target, better quality of life can be acquired by performing TKA via the MMV approach. In addition, compared with MPP approach, the MMV approach could offer less pain and faster recovery.
Comparison of Paraspinal Muscle Degeneration and Decompression Effect Between Conventional Open and Minimal Invasive Approaches for Posterior Lumbar Spine Surgery: a prospective cohort study.
Authors: FU CHEN-JU , Chen Wen-Chien , Lu Meng-Ling , Cheng Chih-Hsiu , Niu Chi-Chien 
Published: March 2020

Abstract
Background Posterior Instrumented Transforaminal Lumbar Interbody Fusion (TLIF) is used to treat spinal stenosis. Minimally invasive surgery (MIS) can cause less muscle injury than conventional open surgery (COS) The purpose of this study was to compare the degree of postoperative fatty degeneration in the paraspinal muscles and the spinal decompression between COS and MIS based on magnetic resonance imaging (MRI). Methods Forty-six patients received TLIF (21 COS, 25 MIS) from February 2016 to January 2017. Lumbar MRI was performed within 3 months before surgery and 1 year after surgery. The postoperative muscle-fat-index (MFI) change of the paraspinal muscles (multifidus and erector spinae) and the dural sac cross-sectional area (DSCAS) change were compared between the 2 groups. Results The average MFI change at L2-S1 erector spinae muscle was significantly greater in the COS group (27.37 ± 21.37% vs. 14.13 ± 19.19%, P = 0.044). A significant MFI change difference between the COS and MIS group was also found in the erector spinae muscle at the caudal adjacent level (54.47 ± 37.95% vs. 23.60 ± 31.59%, P = 0.016). No significant differences at the operated and cranial adjacent level were found. DSCSA improvement after surgery was significantly greater in the COS group (128.15 ± 39.83 mm2 vs. 78.15 ± 38.5 mm2, P = 0.0005) Conclusion COS is associated with more prominent fatty degeneration of the paraspinal muscles. Statically significant post-operative MFI change was only noted in erector spinae muscle at caudal adjacent level and L2-S1 mean global level. COS produces a greater decompressive effect than MIS.
Finite element analysis of breakage risk of two kinds of sacroiliac screws in unilateral sacral fractures
Authors: Ma Yupeng , Zhao Yong , Zou Dexin , Dong Shengjie , Sun Xiujiang , Cheng Gong , Lian Wei , Zhao Yuchi , Sun Tao , Wang Dan , Zhang Shudong 
Published: March 2020

Abstract
To compare the risk of breakage of lengthened sacroiliac screw and ordinary sacroiliac screw for the treatment of unilateral vertical sacral fractures to provide reference for clinical application. A finite element model of Tile C pelvic ring injury (unilateral type Denis Ⅱ fracture of sacrum) was produced. The sacral fractures were fixed with lengthened sacroiliac screw and ordinary sacroiliac screw in 6 types of models respectively. The maximal Von Mises stresses and stress distribution of the two kinds of screws in the case of standing on both feet were measured and compared. The maximal Von Mises stress of lengthened screw is less than that of ordinary screw. Compared with ordinary screw, the stress distribution in lengthened screw is more homogeneous. In conclusion, the breakage risk of screws fixed in double-segment is lower than that of screws fixed in single-segment, and the breakage risk of lengthened screws is lower than that of ordinary screw, and the breakage risk of screws fixed in S2 segment is lower than that of screws fixed in S1 segment.
Hemivertebra Resection Combined With Wedge Osteotomy for Treatment of Severe Rigid Congenital Kyphoscoliosis in Adolescence
Authors: Yin Xinhua , Yan Liang , He Baorong , Hao Ding Jun , Liu Zhongkai 
Published: March 2020

Abstract
Background There was a controversy about surgery approach of severe rigid congenital kyphoscoliosis in adolescence treatment. Aim of the study is to compare the clinical efficacy of surgical treatement by hemivertebra resection (HR) and hemivertebra resection combined with wedge osteotomy (HRWO) for severe rigid congenital kyphoscoliosis in adolescence. Methods Twenty-five patients with severe rigid congenital kyphoscoliosis between Jan 2006 and Dec 2011 were studied in our center. The patients were divided into hemivertebra resection group (group HR) or hemivertebra resection combined with wedge osteotomy group (group HRWO). The clinical and radiographic evaluation in terms of operation time, blood loss, correction rate, fusion time, hospital stay, complications and SRS-24 questionnaire score were compared between Group A and Group B. Results It is obvious that group HR achieved much better results in time of operation time, intra-operative blood loss, and hospital stay than group HRWO (P < 0.05). But meanwhile, group HRWO was significantly better than group HR in the times of coronal Cobb angle, kyphosis, the sagittal imbalance, coronal imbalance and SRS-24 questionnaire score (P < 0.05). There were no significant differences between the two groups in the age, mean flexibility, follow-up time, fusion time, and complications in the last follow-up (P > 0.05). Conclusion The outcomes of follow-up showed that the hemivertebra resection combined with wedge osteotomy approach obtained better clinical outcomes hemivertebra resection surgery. It might be a better surgical treatment for severe rigid congenital kyphoscoliosis in adolescence patients, but it needs longer operation time, more intra-operative blood loss, and extended hospital stay.
Simulation of Intelligent Robot in Boxing Exercises Based on Kinect Sensor
Authors: Li Peicong 
Published: March 2020

Abstract
In the continuous development process, robot technology has been widely used in aerospace, medical, education and service industries, and the relationship between robots and humans is getting closer.The improvement of robot intelligence is a process of continuously learning outside knowledge.Since the introduction of human-computer interaction technology in 1959, it has provided more and more technical support for human research robots.This paper first takes Taijiquan as an example, and carries out mechanical analysis and deconstruction of its standard Taiji pusher action, and obtains corresponding action parameterization information;Then, based on the human-computer interaction technology, the human body depth image and the skeleton structure are acquired through the Kinect sensor, and the human joint position data is recorded.The coordinate conversion is sent to the Nao robot, which realizes the imitation learning of the robot's push action on the Taiji.
Application of data mining technology and wireless network sensing technology in sports training index analysis
Authors: Qian Liqiu , Liu Jiatong 
Published: March 2020

Abstract
The conventional analysis method can provide a general analysis of sports training index, but its ability is relatively low when analyzing niche data. To solve this problem, this paper proposes data mining technology. First, the indicator parameter classification is determined, then the data mining technology is imported, the sports training analysis mechanism is established through this technology, and the construction of the index analysis model is completed. The model is used to analyze the process of niche data mining, and effective data of training indicators are obtained. Deep learning is a method of machine learning based on representation of data.Through the coverage test, accuracy test and immunity test, the variable parameters of the comprehensive analysis capability are determined. Further calculation of this parameter shows that the comprehensive ability of the data mining application analysis method is improved by 37.14% compared with the conventional method, which is suitable for analysis of niche sports training indicators of different data types.
Assessment of the Role of a Tutor in Implementation of Individual Educational Strategy of a University Applicant
Authors: Bogomolov Denis , Grinev Alexandr , Berechikidze Iza , Larina Svetlana , Degtyarevskaya Tatyana 
Published: March 2020

Abstract
Background Nowadays the specialist program of medical profile is one of the most popular admission programs among university entrants. The study is aimed at assessment of the tutor's role in the implementation of the applicant's individual educational strategy basing on the example of preparation for the exam in biology for admission to a medical university. Methods The study is basing on the hypothesis that the tutor is an important, but not essential component of an individual educational strategy for entering a medical university. More than 100 first-year students of the Sechenov University took part in the questionnaire survey to implement the goals and objectives of the research. The survey results were analyzed using specialized statistical packages. Standard methods of descriptive statistics, correlation analysis, clustering methods were used. Results As a result various IES were identified and several features of the interconnection of the IES and the results of USE were studied.In the framework of this study, various educational strategies that are implemented by the student were identified and analyzed, including the students of schools participating in the program “Medical class in Moscow school” and “Sechenov Pre-university”, so, it gives an opportunity to assess the effectiveness of profile orientation within the biological profile and to extrapolate findings to similar programs of profile orientation of education in high school. Conclusions This study provides an opportunity to assess the effectiveness of a particular educational strategy and can help to choose the right path in preparation for admission to medical university. Trial registration Retrospectively registered.
Silver nanoparticles as a potential nematicide against Meloidogyne graminicola
Authors: Baronia Richa , Kumar Puneet , Singh S. P. , Walia R. K. 
Published: March 2020

Abstract
Plant-parasitic nematodes cause severe damage to the various agricultural crops, leading to economic losses for farmers. Therefore, identification and development of novel and environmentally benign nematicides is critically important. In this study, a silver nanoparticle (AgNP) formulation was synthesized, characterized, and investigated as a potential nematicide against rice root-knot nematode, Meloidogyne gramnicola, on rice (Oryza sativa). A series of lab assays (water and sand screening) and glasshouse experiments (using soilless system, autoclaved soil, and naturally infested soil) were conducted to examine the nematicidal effects of AgNP. The results from lab assays revealed 0.1 µg/ml as the minimum concentration for 100% irreversible nematode mortality after 12 hr in the water screening test. However, results from the sand screening test indicated 100% nematicidal effect of AgNP at 2 µg/ml after 24 hr of incubation. In glasshouse assays in soilless system of rice cultivation, 1 µg/ml concentration of AgNP applied directly to the trays achieved significant suppression of root gall formation. The effective dosage to kill nematodes in field soil assays was determined to be 3 µg/ml, which is lower than the value of 150 µg/ml reported in the literature. No visible adverse effect of AgNP was observed on seed germination or plant growth in all the experiments. The results indicate that AgNP has effective nematicidal activity against M. graminicola in rice.
A draft genome of a field-collected Steinernema feltiae strain NW
Authors: Fu Zhen , Li Yuxiang , Elling Axel A. , Snyder William E. 
Published: March 2020

Abstract
Advances in sequencing technologies have accelerated our understanding of the complex genetic network of organisms and genomic divergences that are linked to evolutionary processes. While many model organisms and laboratory strains have been sequenced, wild populations are underrepresented in the growing list of sequenced genomes. Here, we present a de novo assembly of Steinernema feltiae, strain NW, collected from a working agricultural field in south central Washington, USA. Leveraging Pacific Biosciences (PacBio) long reads, we sequenced strain NW to a high depth (99×). The resulting de novo assembly is significantly larger than the previous assembly generated from the laboratory strain SN, with a noticeable improvement in continuity and completeness. Comparative analysis of two assemblies revealed numerous single nucleotide polymorphisms (SNPs), breakpoints, and indels present between the two genomes. This alternative genome resource and annotation could benefit the research community to examine the genetic foundation of evolutionary processes as well as genomic variation among conspecific populations.
Essential oils for managing Pratylenchus penetrans on Easter lilies
Authors: Westerdahl B. B. , Giraud D. , Riddle L. J. , Anderson C. A. 
Published: March 2020

Abstract
Easter lily bulbs for greenhouse forcing are produced in Del Norte County, California and Curry County, Oregon, USA. Pratylenchus penetrans infestation seriously affects growth of field grown bulbs. During two consecutive years of field trials containing 22 treatments, commercially prepared formulations of essential oils (EOs) were compared to an untreated control and to a standard chemical fumigant treatment (FU) (1,3-dichloropropene and metam sodium) applied preplant followed by phorate (PH) at planting to determine their value in the management of lesion nematode, and in improving plant health. The EO products Duogard, EF400, EF300, and Cinnamite were tested as preplant dips to bulblet planting stock. The treated bulblets were tested either alone, in combination with PH at-planting, at planting following FU or in combination with PH at planting following FU. The organophosphates ethoprop and fosthiazate were also tested either alone, or at a reduced rate in combination with a reduced rate of PH. With respect to bulb circumference, ten treatments consistently outperformed the control. In consecutive years, three treatments had healthier looking roots than the control. At harvest, levels of lesion nematode within roots were consistently lower in nine treatments. EOs were beneficial in mitigating nematode damage.
Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease
Authors: Carta L.K. , Li S. 
Published: March 2020

Abstract
Generating DNA markers for microscopic plant parasitic nematodes can be especially difficult if only a few valuable, tiny specimens are available. Providing a reliable maximum amount of unambiguous genetic information from single nematodes is especially important when identifying damaging, regulated nematodes of importance to trade where a few nucleotide differences in diagnostic markers are significant. There are many possible reasons for difficulty amplifying unpurified nematode DNA for long range PCR followed by direct sequencing. Specimen age, proofreading errors and reagent compatibility during PCR are among those problems. While unsuccessful direct amplification of difficult samples may sometimes be overcome by cloning, a more expensive and time-consuming process. Therefore, long segment PCR of a large 3.5 kb segment of ribosomal DNA was optimized for individual difficult-to-amplify young Litylenchus crenatae mccannii (Anguinidae) nematodes by systematically testing thermostable polymerases, proofreading enzymes and buffers. The combination of thermostable DreamTaq™, proofreading Pfu polymerase, and PicoMaxx™ buffer provided the best results. These nematodes are the subject of surveys currently active at many sites in the northeastern United States. This new, optimized PCR protocol will be useful for diagnostic labs associated with the surveys.
Fungal communities associated with Heterodera glycines and their potential in biological control: a current update
Authors: Haarith Deepak , Bushley Kathryn E. , Chen Senyu 
Published: March 2020

Abstract
The soybean cyst nematode (SCN) is the most important pest on soybean, a major crop worldwide. The SCN is considered both parasitic and pathogenic as it derives nutrition from the host and manipulates host physiology to do so. Currently, there are no commercially available chemicals that are specific, environmentally safe and cost effective to control SCN levels. Crop rotation, use of host resistance and other cultural practices remain the main management strategies. The need for bioprospecting other methods of controlling SCN is paramount, and fungi show promise in that respect. Several studies have evaluated fungi and fungal products as biocontrol options against plant-parasitic nematodes. This review discusses fungal genera isolated from the SCN with potential for use as biocontrol agents and the effects of their secondary metabolites on various stages of SCN development. The review also summarizes efforts to control SCN using soil amendments that could potentially impact fungal communities in the soil.
Accidente Cerebrovascular Secundario a Síndrome de HELLP y sus Implicaciones Cognitivas
Authors: Martínez Arrieta Karen Tatiana , Vargas Rubio Claudia Victoria , Acosta María Rocio 
Published: March 2020

El accidente cerebrovascular es una afección clínica que tiene entre uno de sus factores de riesgo la presión arterial alta. En la gestación, la complejidad de esta afección puede desembocar en un cuadro hipertensivo severo, comúnmente conocido como síndrome de HELLP, el cual es una de las variaciones más complejas de la hipertensión gestacional, que conlleva a múltiples alteraciones en la salud de aquellas que lo padecen. La mayoría de los estudios en el área se han enfocado en conocer su etiología, epidemiología y las consecuencias físicas que acarrean este síndrome; sin embargo, poca es la información que se tiene sobre las secuelas cognitivas a largo plazo que produce en la madre un embarazo complicado posterior a un cuadro hipertensivo severo. El presente estudio tuvo como objetivo analizar el caso de una mujer, quien presentó síndrome de HELLP con una complicación de encefalopatía en tallo cerebral y su posterior perfil cognitivo. Se realizó un análisis descriptivo de exploración neuropsicológica y se compararon sus resultados con datos normativos. Entre los resultados se encontraron dificultades en todas las áreas del funcionamiento, además de un déficit cognitivo secundario al cuadro clínico de base. Se concluyó que la hipertensión gestacional es más severa cuando se acompaña de una afectación vascular. Esto afecta la cognición, teniendo un impacto negativo en la vida de la mujer en sus diferentes áreas de ajuste.
Improving hydropower inflow forecasts by assimilating snow data
Authors: Magnusson Jan , Nævdal Geir , Matt Felix , Burkhart John F. , Winstral Adam 
Published: March 2020

AbstractAccurate long-term inflow forecasts are essential for optimal planning of hydropower production. In snow-rich regions, where spring snowmelt is often the largest reservoir of water, inflow forecasts may be improved by assimilating snow observations to achieve more accurate initial states for the hydrological models prior to the prognosis. In this study, we test whether an ensemble Kalman based approach is useful for this purpose for a mountainous catchment in Norway. For 15 years, annual snow observations near peak accumulation at three locations were assimilated into a distributed hydrological model. After the update, the model was run for a 4-month forecasting period with inflows compared to a base case scenario that omitted the snow observations. The assimilation framework improved the forecasts in several years, and in two of the years, the improvement was very large compared to the base case simulation. At the same time, the filter did not degrade the forecasts largely, indicating that though the updating might slightly degrade performance in some years, it maintains the potential for large improvements in others. Thus, the framework proposed here is a viable method for improving snow-related deficiencies in the initial states, which translates to better forecasts.