bluuebunny/crossref_metadata_2025_split huggingface dataset:
INSTITUIÇÃO DE UM PROTOCOLO DE VIGILÂNCIA ALIMENTAR E NUTRICIONAL NO MUNICÍPIO DE JAGUARI-RS
Authors: Taschetto Hailê Zeul Panisson , Medeiros Teles , Costenaro Regina Gema Santini , Haeffner Léris Salete , Benedetti Franceliane Jobim 
Published: November 2020

Os controles periódicos antropométricos do Programa Bolsa Família (PBF) devem seguir os passos preconizados pelo protocolo do Sistema de Vigilância Alimentar e Nutricional (SISVAN). Mas, observou-se que este protocolo não estava sendo aplicado em sua plenitude no município de Jaguari-RS, perdendo-se a oportunidade de identificar as crianças com distúrbios nutricionais e tomar as medidas pertinentes a cada caso. Com o objetivo de implementar um protocolo de Vigilância Alimentar e Nutricional (VAN) que seja efetivamente aplicado, foi desenvolvida uma pesquisa-ação. Após feito o diagnóstico do funcionamento/operacionalidade do SISVAN e conhecida a situação nutricional das crianças beneficiárias do PBF, público-alvo, foram implementadas alterações operacionais nas avaliações antropométricas semestrais e realizada capacitação antropométrica dos profissionais de saúde. A monitorização inicial, realizada durante um período de controle antropométrico do PBF, revelou resultados positivos na implementação do protocolo de VAN.
Regular nutritional assessment is one condicionality of the Bolsa Familia Program (BFP) that must follow all recommended steps of Brazilian Food and Nutrition Surveillance System (SISVAN) protocol. However, it was observed that protocol was not accomplished in all extension in the city of Jaguari- RS, south of Brazil. That way, it was leaving out the opportunity for identifying children nutritional disorders and act in order to take adequate measures. An action research was performed in order to effectively implement a food and nutrition surveillance protocol (FNSP). First, it was verified the way SISVAN was been operated and it was also checked nutritional status from children enrolled in BFP. Then, operating changings were set up in anthropometric assessments and it was realized a training in anthropometry for health professionals. Monitoring performed during an anthropometric assessment showed positive results in the process of FNSP implementation.
REPRESENTAÇÕES SOCIAIS DE IDOSOS INSTITUCIONALIZADOS ACERCA DA PROMOÇÃO DA SAÚDE POR MEIO DE AÇÕES DIALÓGICAS
Authors: De Carvalho Telma Pelaes , Willig Mariluci Hautsch , Kalinke Luciana Puchalski , Ziesemer Nadine Biagi Sousa , Berberian Ana Paula , Massi Giselle Aparecida Athayde 
Published: November 2020

Objetivo: conhecer as representações sociais de idosos institucionalizados por meio de ações dialógicas. Método: estudo qualitativo descritivo, ancorado na Teoria das Representações Sociais. Utilizou-se de encontros dialógicos grupais de promoção à saúde e entrevistas semiestruturadas. Os resultados foram processados pelo software IRAMUTEQ. Resultados: dentre participantes, a maioria era constituída por mulheres, com idade média de 77 anos, 06 eram viúvas, a escolaridade predominante foi o ensino fundamental incompleto, o tempo de residência nas ILPIs variou de um a nove anos.O software concentrou as respostas dos idosos das entrevistas em três classes, assim nominadas: 1) Conversar para conhecer as outras pessoas, conviver com elas e me fortalecer; 2) Participar do encontro_reunião ajuda a enfrentar a vida institucionalizada; 3) Nunca é tarde para cuidar da saúde. Considerações Finais: os enunciados elaborados pelos participantes revelam que a interação social promove a produção e a troca de conhecimento, o fortalecimento de relações interpessoais, o compartilhamento de experiências vividas e o estabelecimento de confiança mútua. As atividades mostraram-se eficazes para aos idosos, ajudando-os a (re) significar a vida institucionalizada.
PERFIL EPIDEMIOLÓGICO DE PACIENTES ATENDIDOS NA CLÍNICA DE FISIOTERAPIA DE UM FRIGORÍFICO: UM ESTUDO RETROSPECTIVO
Authors: Borges Yasmin Dos Santos , Bagatini Maria Amélia , Galvan Tatiana Cecagno 
Published: November 2020

Objetivo: Traçar um perfil epidemiológico de pacientes/funcionários atendidos na clínica de fisioterapia de um frigorífico do Rio Grande do Sul. Método: A pesquisa caracteriza-se como observacional de caráter retrospectivo. A população foi composta por 49 fichas de atendimento. O critério de inclusão foi possuir fichas de atendimento fisioterapêutico completas e os critérios de exclusão foram possuir fichas incompletas, ou possuir fichas repetidas (fichas de reavaliação). Todas as análises estatísticas foram realizadas no programa SPSS versão 8.0 e p significativo quando menor que 0,05. Resultados: Dentre os pacientes, identificou-se prevalência do sexo masculino, casados, com mediana de idade em 36 anos e baixo nível de escolaridade. O setor de produção foi o que apresentou mais queixas (46,9%) e os segmentos mais apontados com presença de desconforto foi o membro superior (46,7%). Analisando as comparações identificou-se significância entre local da queixa e sexo do trabalhador, onde mulheres têm mais queixa de dor torácica (p=0,044) e cervical (p=0,053) em relação aos homens. Conclusão: Esses dados demonstram a necessidade da fisioterapia preventiva para auxiliar no diagnóstico das causas destes desconfortos com posterior plano de ação para erradica-los. Sugere-se que sejam realizados novos estudos de caráter epidemiológico para assim criar um perfil mais amplo e fidedigno, visto que a amostra desse estudo foi pequena. Também sugere-se novos estudos verificando quais os motivos dos desconfortos estarem ocorrendo com maior frequência neste segmento.
Impact of climate change on the hydrology of a semi-arid river basin of India under hypothetical and projected climate change scenarios
Authors: Desai Sujeet , Singh D. K. , Islam Adlul , Sarangi A. 
Published: November 2020

AbstractClimate change impact on the hydrology of the Betwa river basin, located in the semi-arid region of Central India, was assessed using the Soil and Water Assessment Tool (SWAT), driven by hypothetical scenarios and Model of Interdisciplinary Research on Climate version 5 (MIROC5) Global Circulation Model projections. SWAT-Calibration and Uncertainty Programs (SWAT-CUP) was used for calibration and validation of SWAT using multi-site streamflow data. The coefficient of determination, Nash–Sutcliffe efficiency, RMSE-observations standard deviation ratio and percent bias during calibration and validation period varied from 0.83–0.92, 0.6–0.91, 0.3–0.63 and −19.8–19.3, respectively. MIROC5 projections revealed an increase in annual mean temperature in the range of 0.7–0.9 °C, 1.2–2.0 °C and 1.1–3.1 °C during the 2020s, 2050s, and 2080s, respectively. Rainfall is likely to increase in the range of 0.4–9.1% and 5.7–15.3% during the 2050s and 2080s, respectively. Simulation results indicated 3.8–29% and 12–48% increase in mean annual surface runoff during the 2050s and 2080s, respectively. Similarly, an increase of 0.2–3.0%, 2.6–4.2% and 3.5–6.2% in mean annual evapotranspiration is likely during the 2020s, 2050s and 2080s, respectively. These results could be used for developing suitable climate change adaptation plans for the river basin.
Evaluation of bacterial contamination and mutagenic potential of treated wastewater from Al-Samra wastewater treatment plant in Jordan
Authors: AL-Quraan Nisreen A. , Abu-Rub Lubna I. , Sallal Abdel-Kareem 
Published: November 2020

Abstract
Jordan is one of the lowest countries in the world in terms of water resources. The reuse of treated wastewater is an important alternative to supply agricultural demands for water. In Jordan, Kherbet Al-Samra wastewater treatment plant (KSWWTP) is the largest and its effluent is mainly used for irrigation purposes. In this study, bacterial contamination and mutagenic potential were evaluated in six sites, beginning with KSWWTP and ending with King Tallal Dam. The results showed high contamination with many pathogenic bacteria and coliforms. The isolated pathogenic bacteria were Salmonella sp., Shigella sp., Bacillus cereus and Staphylococcus aureus. The isolated opportunistic pathogenic bacteria were Acinetobacter lwoffii, Elizabethkingia meningosepticum, Pseudomonas fluorescens and Bacillus licheniformis. These bacteria were found in all sampling sites without a specific prevalence pattern. Differences in temperature between seasons affect total coliform and other bacterial count. All water samples showed positive mutagenic activity and high bacterial pollution. Improving the disinfection efficiency in the wastewater treatment plant is important to minimize potential toxicity and exposure of public health to pathogenic bacteria, reduce water resources' contamination and environmental pollution. Increasing effluent sampling frequency from KSWWTP is required to monitor bacterial contamination and toxicity/mutagenicity level for water safety and public health risk assessments.
Mesoporous structure regulation of activated carbons and effects on the synergistic efficacy of synchronous adsorption and bio-degradation in biological activated carbon process
Authors: Gong Xu-Jin , Dong Yu-Qi , Li Wei-Guang 
Published: November 2020

Abstract
Mesoporous activated carbon MCGL-4 was tailored for simultaneous enhancement of adsorption and bio-degradation by multistage depth-activation (MDA). Synergistic efficacy of synchronous adsorption and bio-degradation was evaluated in pilot-scale bio-enhanced activated carbon (BEAC) system. Results identified that MCGL-4 obtains synchronously well-developed meso- (0.7605 cm3/g), micro- (0.2655 cm3/g) and macro-porous (0.143 cm3/g) structures. Higher volume during 20.4–208.2 Å (0.6848 cm3/g) ensured higher adsorption capacities for natural organic matters (NOM). The initial immobilized biomass and stabilities on MCGL-4 were also significantly promoted. Rapid small-scale column tests system (RSSCTs) tests showed that adsorption capacities for humic-like organics were 67,725.32 mg·DOC/(kg·carbon) at 39.50 m3·H2O/(kg·carbon). In BEAC system, MCGL-4 achieved higher removal efficiency for fulvic acid, humic acid and aromatic organic matters than commercial carbons. At 39.50 m3·H2O/(kg·carbon), cumulative uptake of organic-pollutants achieved by MCGL-4 was 94,850.51 mg·DOC/(kg·carbon). The proportion occupied by bio-degradation were 31,674.70 mg·DOC/(kg·carbon). It also confirmed that bio-degradation ability was much higher than commercial carbons after mesoporous structures regulation by MDA process.
Evaluation of a human-associated genetic marker for Escherichia coli (H8) for fecal source tracking in Thailand
Authors: Nopprapun Pimchanok , Boontanon Suwanna Kitpati , Harada Hidenori , Surinkul Nawatch , Fujii Shigeo 
Published: November 2020

Abstract
High levels of microbial fecal pollution are a major concern in many countries. A human-associated genetic marker for Escherichia coli (H8) has recently been developed for fecal source tracking. The assessment of the H8 marker performance is crucial before it can be applied as a suitable method for fecal source tracking in each country. The performance (specificity and sensitivity) of the H8 marker was evaluated by using non-target host groups (cattle, buffalo, chicken, duck, and pig feces) and target host groups (influent and effluent from a wastewater treatment plant and septages). SYBR based real-time PCR (polymerase chain reaction) was done on 400 E. coli isolates from non-target and target host groups after E. coli isolation. It was found that the specificity from animal feces samples collected in Thailand was 96%. Moreover, influent, effluent, and septage samples showed the values of the sensitivity at 18, 12, and 36%, respectively. All of the non-target host groups were found to be significantly different with positive proportions from the target host group (septage samples) (p ≤ 0.01). Based on the results, this marker is recommended for use as a human-associated E. coli marker for identifying sources of fecal pollution in Thailand.
The Role of MIF in Hepatic Function, Oxidative Stress, and Inflammation in Thioacetamide-induced Liver Injury in Mice: Protective Effects of Betaine
Authors: Vukićević Dušan , Rovčanin Branislav , Gopčević Kristina , Stanković Sanja , Vučević Danijela , Jorgačević Bojan , Mladenović Dušan , Vesković Milena , Samardžić Janko , Ješić Rada , Radosavljević Tatjana 
Published: November 2020

Background:
Macrophage migration inhibitory factor (MIF) is a multipotent
cytokine that contributes to the inflammatory response to chemical liver injury. This cytokine
exhibits pro- and anti-inflammatory effects depending on the etiology and stage of
liver disease.

Objective:
Our study aimed to investigate the role of MIF in oxidative stress and inflammation
in the liver, and modulatory effects of betaine on MIF in thioacetamide (TAA)-induced
chronic hepatic damage in mice.

Methods:
The experiment was performed on wild type and knockout MIF-/- C57BL/6
mice. They were divided into the following groups: control; Bet-group that received betaine
(2% wt/v dissolved in drinking water); MIF-/- mice group; MIF-/-+Bet; TAA-group
that received TAA (200 mg/kg b.w.), intraperitoneally, 3x/week/8 weeks); TAA+Bet;
MIF-/-+TAA, and MIF-/-+TAA+Bet. In TAA- and Bet-treated groups, animals received
the same doses. After eight weeks of treatment, blood samples were collected for biochemical
analysis, and liver specimens were prepared for the assessment of parameters
of oxidative stress and inflammation.

Results:
In MIF-/-mice, TAA reduced transaminases, γ-glutamyltranspeptidase, bilirubin,
malondialdehyde (MDA), oxidative protein products (AOPP), total oxidant status (TOS),
C-reactive protein (CRP), IL-6, IFN-γ, and increased thiols and total antioxidant status
(TAS). Betaine attenuated the mechanism of MIF and mediated effects in TAA-induced
liver injury, reducing transaminases, γ-glutamyltranspeptidase, bilirubin, MDA, AOPP,
TOS, CRP, IL-6, IFN-g, and increasing thiols.

Conclusion:
MIF is a mediator in hepatotoxic, pro-oxidative, and proinflammatoryeffects
of TAA-induced liver injury. MIF-targeted therapy can potentially mitigate oxidative
stress and inflammation in the liver, but the exact mechanism of its action requires
further investigation. Betaine increases anti-oxidative defense and attenuates hepatotoxic
effects of MIF, suggesting that betaine can be used for the prevention and treatment of
liver damage.
Insights from Pharmaceutical Biotechnology into Phenolic Biopharmaceuticals against COVID-19
Authors: Cabral-Hipólito Nidia , Molina-Ramírez Brenda , María-de-la-Luz Sevilla-González  , Meza-Velázquez Rocío , García-Garza Rubén , Velázquez Gauna Sergio-Everardo , Castillo-Maldonado Irais , Delgadillo-Guzmán Dealmy , Rivera-Guillén Mario-Alberto , Serrano-Gallardo Luis-Benjamín , María-del-Carmen Vega-Menchaca  , Ramírez-Moreno Agustina , Pedroza-Escobar David 
Published: November 2020

Background:
The COVID-19 pandemic had infected more than 3.5M people around the
world and more than 250K people died in 187 countries by May 2020. The causal agent of this disease
is a coronavirus whose onset of symptoms to death range from 6 to 41 days with a median of 14 days.
This period is dependent on several factors such as the presence of comorbidities, age and the efficiency
of the innate or adaptive immune responses.

Methods:
The effector mechanisms of both types of immune responses depend on the pathogen involved.
In the case of a viral infection, the innate immune response may approach the harmful virus
through pattern recognition receptors inducing an antiviral state.

Results:
On the other hand, the adaptive immune response activates antibody production to neutralize
or eliminate the virus. Phenolics are plant secondary metabolites with many biological activities for
plants and humans against infection. Chemical modification of proteins may enhance their biological
properties; thus, a protein of medical interest, for instance, a viral protein can be used as a scaffold to
build a biopharmaceutical conjugated or complexated with phenolics exhibiting structural complexity
or biological activities to achieve effective phenolic-protein-based therapeutics like vaccine adjuvant
complexes, immunogen conjugates, and antiviral conjugates.

Conclusion:
Pharmaceutical biotechnology applies the principles of biotechnology to develop biopharmaceuticals
for protein-based therapeutics; such as adjuvants, recombinant proteins, monoclonal
antibodies, and antivirals. As neither a vaccine nor a treatment for COVID-19 is currently available,
this manuscript focuses on insights from pharmaceutical biotechnology into phenolic biopharmaceuticals
against COVID-19.
Design, Synthesis and Primary Immunologic Evaluation of M2e-CRM197 Conjugate as a Universal Influenza Vaccine Candidate
Authors: Xu Lu , Zhang Chun , Zhang Jing , Yu Rong , Su Zhiguo 
Published: November 2020

Background:
Influenza is a contagious respiratory illness caused by an acute infection of
influenza viruses, among which influenza A virus causes seasonal epidemic infections nearly every
year. Due to the unpredictability of the evolving influenza A virus and time-consuming vaccine development
cycles, novel universal influenza vaccines designed to induce broadly cross-reactive immune
responses against frequently mutant influenza A virus strains are urgently required.

Objective:
The aim of this study was to synthesize a novel vaccine through the dual-site specific
conjugation of the constant epitope of 23 amino acids (M2e) of the influenza A virus with a highly
immunogenic carrier protein of Cross-Reacting Material (CRM197) under denaturation and to evaluate
its primary immunogenicity in mice.

Methods:
The antigen (M2e) and the carrier protein (CRM197) were linked with different types of
hetero-functionalized linkers, α-Maleimide-ε-Hydrazide Polyethylene Glycol 2k (MAL-PEG-HZ)
and N-β-Maleimidopropionic Acid Hydrazide (BMPH) separately. The immunogenicity of the
M2e-CRM197 conjugates with different types of linkers was evaluated in mice, and the M2especific
total IgG and IgG-isotypes were determined by ELISA.

Results:
Immunogenicity studies revealed that anti-M2e antibody could be induced by the conjugate
products, M2e-PEG-CRM197 and M2e-BMPH-CRM197, by approximately 30 and 90-fold higher
than that of the M2e group. In addition, the anti-M2e antibody level induced by M2e-PEG-CRM197
conjugate was three times higher than that of M2e-BMPH-CRM197 conjugate, and the former could
simultaneously activate both cellular and humoral immune responses.

Conclusions:
The M2e-CRM197 conjugated vaccines we synthesized in this study are highly immunogenic
compared with M2e alone. Besides, evidence presented here indicated that the hydrophilic,
non-immunogenic and biocompatible chain of the cross-linker might be a better choice for the development
of a conjugate vaccine.
Recent Advances in Ultrasound-Assisted Synthesis of Nano-Emulsions and their Industrial Applications
Authors: Thakur Parag , Sonawane Shriram , Potoroko Irina , Sonawane Shirish H. 
Published: November 2020

Background:
Colloidal systems are used in various industrial and medical applications.
Nanoparticles have good physio-chemical properties that enhance the performance of the system.
Nano-emulsions can be synthesized by ultrasonication. Ultrasonication is the simplest method as compared
to other available methods. The purpose of this article is to review the recent advances in ultrasound-
assisted nano-emulsion synthesis and challenges regarding the safety of the nano-emulsion.

Methods:
Application of the nano-emulsions in the various industrial and medical processes like drug
delivery process, food industry, pesticide industry and cosmetics industry is also reviewed.

Results:
Ultrasound-assisted synthesis of nano-emulsions is a simple method as compared to other
synthesis methods of the nano-emulsions. Nano-emulsions have potential applications in the cosmetic,
drug delivery, pesticides and industry.

Conclusion:
Fabrication of the nano-emulsions is different for each application and product. Physiochemical
properties should be strictly monitored in the nano-emulsions as they influence the efficiency
of the nano-emulsions greatly. Ostwald ripening is the main phenomenon for the nano-emulsions
mechanism.
Application of Contemporary Neuroproteomic Techniques in Unravelling Neurological Disorders
Authors: Khurana Mallika , Rahman Syed Obaidur , Najmi Abul Kalam , Pottoo Faheem Hyder , Akhtar Md Sayeed 
Published: November 2020

:
Decades of research has stunned us with the very distinctive anatomy and physiology of
our brain, and on the other hand, its complexity has always posed great difficulty in treating its dysfunction
or damage. Understanding the brain under normal and, particularly in the diseased state,
has always been very challenging and would have been impossible without proteomics. Neuroproteomic
techniques have been extensively used for unraveling both dynamics and content of the proteome
of our nervous system. This modern-day investigation and quantification of protein concentration
and expression have given us a platform that enhances our knowledge on disease-associated
processes and pathways modification and also leads to the identification of possible biomarkers
that can be therapeutically targeted. With an increased interest in identifying and targeting possible
biomarkers, this article focuses on describing applications of the much discussed neuroproteomics,
with a significant role in the disease pathogenesis of some very common neurological disorders.
This article will collectively discuss the use and relevance of neuroproteomics in a range of neurological
diseases, such as Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, epilepsy, and
psychiatric disorders. We have also attempted to present the current successes and failures of the
neuroproteomics approach on the results obtained from different clinical studies that targeted biomarkers
associated with any particular neurological disorder.
Novel Carbamοyloxy Analogues of Tamoxifen: Synthesis, Molecular Docking and Bioactivity Evaluation
Authors: Kasiotis Konstantinos M. , Lambrinidis George , Fokialakis Nikolas , Haroutounian Serkos A. 
Published: November 2020

Background:
Tamoxifen (TAM), a non-steroidal antiestrogen, constitutes the endocrine
treatment of choice against breast cancer. Since its inauguration, substantial effort has been devoted
towards the design and synthesis of TAM’s analogues aiming to improve its bioactivity and reveal
their structure-activity relationship.

Objective:
One of the most studied synthetic features of TAM’s structure is the ether side chain,
which is strongly related to its positioning into the active site of the Estrogen Receptors (ERα and
ERβ). Herein, we present the application of a straightforward route for the efficient synthesis of
selected novel carbamoyloxy analogues of TAM and the evaluation of their respective binding affinities
to the Estrogen Receptors α and β.

Methods:
A one-pot reaction was applied for the construction of TAM’s triarylethylene core moiety,
which subsequently was derivatized to provide efficiently the target carbamoyloxy analogues of
TAM. The Z and E isomers of the latter were separated using RP-HPLC-UV and their binding affinities
to ERα and ERβ were measured.

Results:
Among all compounds synthesized, the dimethyl derivative was determined as the most
potent for both receptors, displaying binding affinity values comparable to TAM, though the Zdiethyl
analogue maintained substantial affinity to both ERs. The aforementioned results were further
studied by theoretical calculations and molecular modelling to delineate a concordance among
calculations and biological activity.

Conclusion:
Approach applied herein permitted the extraction of a useful structure-activity relationship
correlation pattern highlighting the importance of a chemically stabilized tamoxifen side chain.