bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular characterization of a Trichinella spiralis serine proteinase
Authors: Yue Xin , Sun Xiang Yuan , Liu Fang , Hu Chen Xi , Bai Ying , Da Yang Qi , Liu Ruo Dan , Zhang Xi , Cui Jing , Wang Zhong Quan 
Published: September 2020

AbstractThe aim of this study was to investigate the biological characteristics and functions of a Trichinella spiralis serine proteinase (TsSerp) during larval invasion and development in the host. The full-length TsSerp cDNA sequence was cloned and expressed in Escherichia coli BL21. The results of RT-PCR, IFA and western blotting analyses showed that TsSerp was a secretory protein that was highly expressed at the T. spiralis intestinal infective larva and muscle larva stages and primarily located at the cuticle, stichosome and intrauterine embryos of the parasite. rTsSerp promoted the larval invasion of intestinal epithelial cells (IECs) and the enteric mucosa, whereas an anti-rTsSerp antibody impeded larval invasion; the promotion and obstruction roles were dose-dependently related to rTsSerp and the anti-rTsSerp antibodies, respectively. Vaccination of mice with rTsSerp elicited a remarkable humoral immune response (high levels of serum IgG, IgG1/IgG2a, IgE and IgM), and it also triggered both systemic (spleen) and local intestinal mucosal mesenteric lymph node (MLN) cellular immune responses, as demonstrated by a significant elevation in Th1 cytokines (IFN-γ) and Th2 cytokines (IL-4) after the spleen and MLN cells from vaccinated mice were stimulated with rTsSerp. Anti-TsSerp antibodies participated in the killing and destruction of newborn larvae via ADCC. The mice vaccinated with rTsSerp exhibited a 48.7% reduction in intestinal adult worms and a 52.5% reduction in muscle larvae. These results indicated that TsSerp participates in T. spiralis invasion and development in the host and might be considered a potential candidate target antigen to develop oral polyvalent preventive vaccines against Trichinella infection.
Association of working conditions including digital technology use and systemic inflammation among employees: study protocol for a systematic review
Authors: Kaltenegger Helena C. , Becker Linda , Rohleder Nicolas , Nowak Dennis , Weigl Matthias 
Published: September 2020

AbstractBackgroundWith the dynamic advancement of digitalization, working environments are changing and risk for employee stress may be increasing. Work stress has been associated with a dysregulation of inflammatory processes as a component of immune function. Systemic low-grade inflammation is discussed as a key player in the relation between stress exposure and chronic illness, such as cardiovascular diseases. The objective of this investigation will be to evaluate the association of working conditions including digital technology use and systemic inflammation among employees.MethodsWe designed and registered a study protocol for a systematic review of randomized controlled trials and prospective non-randomized studies (e.g., cohort, interrupted time series, or before-after studies). We will include studies conducted among adult workers reporting associations of working conditions and inflammatory activity. The outcome will be biomarkers of systemic low-grade inflammation on cell, plasma molecule and intracellular level, such as C-reactive protein, or different types of leukocytes, cytokines, etc. Literature searches will be conducted in several electronic databases (from January 1982 onwards), including PubMed/MEDLINE, Embase, PsycINFO, Web of Science, and CENTRAL. Two reviewers will independently screen all retrieved records, full-text articles, and extract data. The study methodological quality (or bias) will be appraised using appropriate tools. Our results will be described qualitatively. Random effects meta-analysis will be conducted, if feasible and appropriate. Additional analyses will be performed to explore potential sources of heterogeneity.DiscussionThis systematic review and meta-analysis will provide a synthesis of studies evaluating the association of working conditions and systemic inflammation. We anticipate our findings to identify knowledge gaps in the literature that future research should address. Moreover, results of our review may provide implications for corporate and public policy action for employee health promotion and prevention of occupational stress.Systematic review registrationPROSPERO ID:CRD42020166887
Hopf bifurcation in a diffusive predator–prey model with Smith growth rate and herd behavior
Authors: Jiang Heping , Fang Huiping , Wu Yongfeng 
Published: September 2020

AbstractThis paper mainly aims to consider the dynamical behaviors of a diffusive delayed predator–prey system with Smith growth and herd behavior subject to the homogeneous Neumann boundary condition. For the analysis of the predator–prey model, we have studied the existence of Hopf bifurcation by analyzing the distribution of the roots of associated characteristic equation. Then we have proved the stability of the periodic solution by calculating the normal form on the center of manifold which is associated to the Hopf bifurcation points. Some numerical simulations are also carried out in order to validate our analysis findings. The implications of our analytical and numerical findings are discussed critically.
Stability and solvability for a class of optimal control problems described by non-instantaneous impulsive differential equations
Authors: Chen Yi , Meng Kaixuan 
Published: September 2020

AbstractIn this paper, we investigate the existence and stability of solutions for a class of optimal control problems with 1-mean equicontinuous controls, and the corresponding state equation is described by non-instantaneous impulsive differential equations. The existence theorem is obtained by the method of minimizing sequence, and the stability results are established by using the related conclusions of set-valued mappings in a suitable metric space. An example with the measurable admissible control set, in which the controls are not continuous, is given in the end.
Assessing farmers' contribution to greenhouse gas emission and the impact of adopting climate-smart agriculture on mitigation
Authors: Israel Michael Ayeah , Amikuzuno Joseph , Danso-Abbeam Gideon 
Published: September 2020

Abstract
Background
The adoption of climate-smart agricultural (CSA) practices is expected to improve farmers’ adaptation to climate change and also increase yields while simultaneously curbing greenhouse gas (GHG) emissions. This paper explores the determinants of smallholder farmers’ participation in GHG-emitting activities. It also estimates the impact of CSA activities on reducing GHG emissions.

Methods
The findings are based on survey data obtained from 350 smallholder farmers in the East Gonja district of Northern Ghana. We adopted the generalized Poisson regression model in identifying factors influencing farmers’ participation in the GHG emission practices and inverse-probability-weighted regression adjustment (IPWRA) to estimate the impact of CSA adoption on GHG emissions.

Results
Most farming households engaged in at least one emission activity. The findings of the generalized Poisson model found that wealthier households, higher education, and households with access to extension services were less likely to participate in GHG emission activities. There was also evidence that CSA adoption significantly reduces GHG emissions.

Conclusion
Advocacy in CSA adoption could be a necessary condition for environmental protection through the reduction of GHG emissions.
Effectiveness of two-stage horizontal subsurface flow constructed wetland planted with Cyperus alternifolius and Typha latifolia in treating anaerobic reactor brewery effluent at different hydraulic residence times
Authors: Alayu Ermias , Leta Seyoum 
Published: September 2020

Abstract
Background
Agro-industrial wastewaters in Ethiopia cause severe environmental pollution problems. Research evidence showed that anaerobic reactors are good options for the treatment of these wastewaters. But, their final effluent does not meet the discharge standards. Conversely, a series stage horizontal subsurface flow constructed wetland (HSSFCW) system is encouraging for the polishing of anaerobic reactor effluents. However, its treatment efficiency is dependent on hydraulic residence time (HRT). Cyperus alternifolius and Typha latifolia-based wastewater treatment showed good removal efficiencies individually. However, data on their combined treatment effectiveness is negligible. Therefore, this study assesses HRT influences on the treatment effectiveness of a two-stage HSSFCW system planted with these two macrophytes for the polishing of anaerobic reactor brewery effluent. A series connected two-stage HSSFCW unit planted with Cyperus alternifolius and Typha latifolia was built to treat Kombolcha brewery anaerobic reactor effluent. Then, the macrophytes were endorsed to grow with continuous application of diluted brewery effluent from a reservoir tank using gravity force. After dense stand formation, the experiment was initiated to determine the influence of HRTs on the removal efficiency of a complete wetland system. The system was operated sequentially by supplying fixed influent inflow rates of 2791, 1395, 930, 698, and 558 L day−1, respectively, for 1, 2, 3, 4, and 5 days HRT. Both the influent and effluent of the two-stage HSSFCW system were analyzed following common procedures for main brewery pollutants.

Result
Results showed that as HRT increased from 1 to 5 days, the effluent pH and temperature were decreased along with enhanced pollutant removals ranging from 47.8–87.2%, 29.2–90.1%, 32.9–77.7%, 16.8–75.4%, and 18.4–76.8% with decreased influent mass loading rates ranging from 26.4–2.1, 64.5–7.3, 11.5–0.8, 5–0.6, and 3.8–0.4 gm−2 day−1, respectively, for total suspended solids, chemical oxygen demand, total nitrogen, total phosphorous and orthophosphate. However, better and steadier pollutant removals were achieved at higher HRTs.

Conclusion
For better nutrient removals, the 4 and 5 days HRT can serve as a good benchmark.
Radiotherapy Practice for Treatment of Bone Metastasis in Ethiopia
Authors: Rick Tara J. , Habtamu Biruk , Tigeneh Wondemagegnhu , Abreha Aynalem , Grover Surbhi , Assefa Mathewos , Heemsbergen Wilma , Incrocci Luca 
Published: September 2020

PUROSE Ethiopia has one cobalt radiotherapy (RT) machine to serve a population of more than 100 million. The purpose of this study was to report on patterns of palliative RT of bone metastasis in a severely low-capacity setting. PATIENTS AND METHODS Patient and treatment characteristics of patients irradiated for palliation of symptomatic bone metastasis were extracted from a retrospective database of patients treated between May 2015 and January 2018. This database included a random sample of 1,823 of the estimated 4,000 patients who were treated with RT within in the study period. Associations between the applied RT schedule and patient and tumor characteristics were evaluated with the χ2 test. Hypothetical savings of RT sessions and time were compared in the case of a single-fraction policy. RESULTS From the database, 234 patients (13%) were treated for bone metastasis. Most patients were ≤ 65 years of age (n = 189; 80%) and female (n = 125; 53%). The most common primary sites were breast (n = 82; 35%) and prostate (n = 36; 15%). Fractionated regimens were preferred over single fraction: 20 Gy in 5 fractions (n = 192; 82.1%), 30 Gy in 10 fractions (n = 7; 3%), and 8 Gy in 1 fraction (n = 28; 12%). Factors associated with single-fraction RT included nonaxial sites of bone metastasis ( P < .01) and an address outside Addis Ababa ( P ≤ .01). If single-fraction RT would have been given uniformly for bone metastasis, this would have resulted in a 78% reduction in the number of RT sessions and 76% reduction in total RT time. CONCLUSION The pattern of palliative RT for bone metastasis in Ethiopia favors fractionated regimens over single fraction. Efforts should be made to adopt evidence-based and cost-effective guidelines.
Venetoclax Plus Rituximab in Relapsed Chronic Lymphocytic Leukemia: 4-Year Results and Evaluation of Impact of Genomic Complexity and Gene Mutations From the MURANO Phase III Study
Authors: Kater Arnon P. , Wu Jenny Qun , Kipps Thomas , Eichhorst Barbara , Hillmen Peter , D’Rozario James , Assouline Sarit , Owen Carolyn , Robak Tadeusz , de la Serna Javier , Jaeger Ulrich , Cartron Guillaume , Montillo Marco , Dubois Julie , Eldering Eric , Mellink Clemens , Van Der Kevie-Kersemaekers Anne-Marie , Kim Su Young , Chyla Brenda , Punnoose Elizabeth , Bolen Christopher R. , Assaf Zoe June , Jiang Yanwen , Wang Jue , Lefebure Marcus , Boyer Michelle , Humphrey Kathryn , Seymour John F. 
Published: September 2020

PURPOSE In previous analyses of the MURANO study, fixed-duration venetoclax plus rituximab (VenR) resulted in improved progression-free survival (PFS) compared with bendamustine plus rituximab (BR) in patients with relapsed or refractory chronic lymphocytic leukemia (CLL). At the 4-year follow-up, we report long-term outcomes, response to subsequent therapies, and the predictive value of molecular and genetic characteristics. PATIENTS AND METHODS Patients with CLL were randomly assigned to 2 years of venetoclax (VenR for the first six cycles) or six cycles of BR. PFS, overall survival (OS), peripheral-blood minimal residual disease (MRD) status, genomic complexity (GC), and gene mutations were assessed. RESULTS Of 389 patients, 194 were assigned to VenR and 195 to BR. Four-year PFS and OS rates were higher with VenR than BR, at 57.3% and 4.6% (hazard ratio [HR], 0.19; 95% CI, 0.14 to 0.25), and 85.3% and 66.8% (HR, 0.41; 95% CI, 0.26 to 0.65), respectively. Undetectable MRD (uMRD) at end of combination therapy (EOCT) was associated with superior PFS compared with low MRD positivity (HR, 0.50) and high MRD positivity (HR, 0.15). Patients in the VenR arm who received ibrutinib as their first therapy after progression (n = 12) had a reported response rate of 100% (10 of 10 evaluable patients); patients subsequently treated with a venetoclax-based regimen (n = 14) had a reported response rate of 55% (six of 11 evaluable patients). With VenR, the uMRD rate at end of treatment (EOT) was lower in patients with GC than in those without GC ( P = .042); higher GC was associated with shorter PFS. Higher MRD positivity rates were seen with BIRC3 and BRAF mutations at EOCT and with TP53, NOTCH1, XPO1, and BRAF mutations at EOT. CONCLUSION Efficacy benefits with fixed-duration VenR are sustained and particularly durable in patients who achieve uMRD. Salvage therapy with ibrutinib after VenR achieved high response rates. Genetic mutations and GC affected MRD rates and PFS.
Enhanced Stromal Cell CBS-H2S Production Promotes Estrogen-Stimulated Human Endometrial Angiogenesis
Authors: Qi Qian-Rong , Lechuga Thomas J , Patel Basari , Nguyen Nicole A , Yang Yi-Hua , Li Yan , Sarnthiyakul Sassi , Zhang Quan-Wei , Bai Jin , Makhoul Josh , Chen Dong-Bao 
Published: September 2020

Abstract
Angiogenesis is a physiological process for endometrial regeneration in the menstrual cycle and remodeling during pregnancy. Endogenous hydrogen sulfide (H2S), produced by cystathionine-β synthase (CBS) and cystathionine-γ lyase (CSE), is a potent proangiogenic factor; yet, whether the H2S system is expressed in the endometrium and whether H2S plays a role in endometrial angiogenesis are unknown. This study was to test whether estrogens stimulate endometrial H2S biosynthesis to promote endometrial microvascular endothelial cell (EMEC) angiogenesis. CBS messenger RNA/protein and H2S production significantly differed among endometria from postmenopausal (POM), premenopausal secretory (sPRM), and proliferative (pPRM) nonpregnant (NP) and pregnant (Preg) women (P &lt; .05) in a rank order of POM approximately equal to sPRM is less than pPRM is less than Preg, positively correlating with angiogenesis indices and endogenous estrogens and with no difference in CSE expression. CBS and CSE proteins were localized to stroma, glands, and vessels in endometrium, and greater stromal CBS protein was observed in the pPRM and Preg states. Estradiol-17β (E2) (but not progesterone) stimulated CBS (but not CSE) expression and H2S production in pPRM endometrial stromal cells (ESCs) in vitro, which were attenuated by ICI 182 780. The H2S donor sodium hydrosulfide promoted in vitro EMEC angiogenesis. Co-culture with sPRM, pPRM, and Preg ESCs all stimulated EMEC migration with a rank order of sPRM less than pPRM approximately equal to Preg. CBS (but not CSE) inhibition attenuated ESC-stimulated EMEC migration. E2 did not affect EMEC migration but potentiated ESC-stimulated EMEC migration. Altogether, estrogens stimulate specific receptor-dependent stromal CBS-H2S production to promote endometrial EMEC angiogenesis in women.
Empiric Determination of the Daily Glucocorticoid Replacement Dose in Adrenal Insufficiency
Authors: Caetano Celina M , Sliwinska Aleksandra , Madhavan Parvathy , Grady James , Malchoff Carl D 
Published: September 2020

Abstract

Background
For the treatment of adrenal insufficiency (AI) in adults, the Endocrine Society’s recommended daily glucocorticoid replacement dose (DGRD) is 15 to 25 mg hydrocortisone (HC), which is approximately 1.7 times the reported mean daily cortisol production rate. Prolonged glucocorticoid overtreatment causes multiple morbidities.

Hypothesis
We tested the hypotheses that the DGRD, empirically determined by individual patient titration, is lower than that of the Endocrine Society guidelines and tolerated without evidence of glucocorticoid under-replacement.

Methods
We empirically determined the DGRD in 25 otherwise healthy adults with AI by titrating the DGRD to the lowest dose tolerated as judged by body mass index, blood pressure, serum sodium concentration and AI symptoms. Patients received either HC or prednisone (PRED). The HC equivalent of PRED was assumed to be 4:1.

Results
The mean empirically determined DGRD, expressed as HC equivalent, was significantly less than the midpoint of the Endocrine Society’s recommended DGRD (7.6 ± 3.5 mg/m2 vs 11.8 mg/m2; P &lt; 0.001). The DGRD in the adrenalectomy group was not significantly different than the DGRD of those with other AI causes (7.9 ± 4.0 mg/m2 vs 7.3 ± 3.1 mg/m2; P = ns), demonstrating that the empirically determined DGRD was not biased by residual cortisol secretion. There was no evidence of glucocorticoid under-replacement as determined by measured biometrics and AI symptoms.

Conclusions
We conclude that an empirically determined DGRD is significantly lower than that of the Endocrine Society guidelines and tolerated without evidence of glucocorticoid under-replacement.
Test Utilization and Value in the Evaluation of Peripheral Neuropathies
Authors: Callaghan Brian C. 
Published: September 2020

ABSTRACT
Peripheral neuropathies can be classified as typical or atypical. Patients with atypical neuropathy have one or more of the following features: acute/subacute onset, non–length dependence, motor predominance, or asymmetry. This classification is important because it informs the appropriate diagnostic evaluation of this highly prevalent condition. The evaluation of a typical peripheral neuropathy, also known as distal symmetric polyneuropathy, requires a thorough history, neurologic examination, and focused laboratory testing. Electrodiagnostic testing and MRI account for the majority of costs but rarely lead to changes in diagnosis or management. These costs are increasingly being passed on to patients, especially those with high-deductible health plans. In contrast, patients with atypical neuropathy require more extensive testing, including electrodiagnostic tests. These tests are much more likely to lead to the use of disease-modifying therapies in these patients compared to in those with typical peripheral neuropathy. This article describes two cases to illustrate the appropriate diagnostic workup of those with typical or atypical neuropathy.
Charcot-Marie-Tooth Disease and Other Hereditary Neuropathies
Authors: Klein Christopher J. 
Published: September 2020

ABSTRACT

PURPOSE OF REVIEW
This article provides an overview of Charcot-Marie-Tooth disease (CMT) and other inherited neuropathies. These disorders encompass a broad spectrum with variable motor, sensory, autonomic, and other organ system involvement. Considerable overlap exists, both phenotypically and genetically, among these separate categories, all eventually exhibiting axonal injury and neurologic impairment. Depending on the specific neural and non-neural localizations, patients experience varying morbidity and mortality. Neurologic evaluations, including neurophysiologic testing, can help diagnose and predict patient disabilities. Diagnosis is often complex, especially when genetic and acquired components overlap.

RECENT FINDINGS
Next-generation sequencing has greatly improved genetic diagnosis, with many third-party reimbursement parties now embracing phenotype-based panel evaluations. Through the advent of comprehensive gene panels, symptoms previously labeled as idiopathic or atypical now have a better chance to receive a specific diagnosis. A definitive molecular diagnosis affords patients improved care and counsel. The new classification scheme for inherited neuropathies emphasizes the causal gene names. A specific genetic diagnosis is important as considerable advances are being made in gene-specific therapeutics. Emerging therapeutic approaches include small molecule chaperones, antisense oligonucleotides, RNA interference, and viral gene delivery therapies. New therapies for hereditary transthyretin amyloidosis and Fabry disease are discussed.

SUMMARY
Comprehensive genetic testing through a next-generation sequencing approach is simplifying diagnostic algorithms and affords significantly improved decision-making processes in neuropathy care. Genetic diagnosis is essential for pathogenic understanding and for gene therapy development. Gene-targeted therapies have begun entering the clinic. Currently, for most inherited neuropathy categories, specific symptomatic management and family counseling remain the mainstays of therapy.
Management of Neuropathic Pain in Polyneuropathy
Authors: Peltier Amanda C. , Wood Derek 
Published: September 2020

ABSTRACT

PURPOSE OF REVIEW
Many polyneuropathies cause significant neuropathic pain, resulting in substantial morbidity and reduced quality of life. Appropriate management is crucial for maintaining quality of life for patients with painful polyneuropathies. The US Food and Drug Administration (FDA) has only approved one new drug for painful diabetic neuropathy in the past decade, a topical capsaicin patch that was initially approved for the treatment of postherpetic neuralgia in 2009. Gabapentinoids and serotonin norepinephrine reuptake inhibitors (SNRIs) continue to have an advantage in safety profiles and efficacy. Other antiepileptic medications remain second-line agents because of fewer studies documenting efficacy.

RECENT FINDINGS
This article reviews recent literature on complementary and pharmacologic therapies for the management of painful polyneuropathies. Exercise has emerged as an important therapeutic tool and may also improve the underlying polyneuropathy in the setting of obesity, metabolic syndrome, and diabetes.

SUMMARY
The approach to management of painful polyneuropathies is multifactorial, using both pharmacologic and nonpharmacologic measures to improve pain severity and patient quality of life.