bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural modeling and conserved epitopes prediction against SARS-COV-2 structural proteins for vaccine development
Authors: Qamar Muhammad Tahir ul , Shahid Farah , Ashfaq Usman Ali , Aslam Sidra , Fatima Israr , Fareed Muhammad Mazhar , Zohaib Ali , Chen Ling-Ling 
Published: February 2020

Abstract
Background: Coronavirus disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Corona virus 2 (SARS-COV-2) was first diagnosed in December 2019, Wuhan, China. Little is known about this new virus and it has the potential to cause severe illness and pneumonia in some people, therefore the development of an effective vaccine is highly desired.Methods: Immunoinformatics and statistical approaches were used in this study to forecast B- and T- cell epitopes for the SARS-COV-2 structural proteins (Surface glycoprotein, Envelope protein, and Membrane glycoprotein) that may play a key role in eliciting immune response against COVID-19. Different types of B cell epitopes (linear as well as discontinuous) and T cell (MHC class I and MHC class II) were determined. Moreover, their antigenicity and allergenicity were also estimated.Results: The antigenic B-cell epitopes exposed to the outer surface were screened out and 23 linear B cell epitopes were selected. “SPTKLNDLCFTNVY” had the highest antigenicity score among B cell epitopes. The T-cell epitopes bound to multiple alleles, antigenic, non-allergen, non-toxic, and conserved in the protein sequence were shortlisted. In total, 16 epitopes (9 from MHC class I and 7 from MHC class II) were selected. Among the T-cell epitopes, MHC class I (IPFAMQMAYRFN) and MHC class II (VTLACFVLAAVYRIN) were classified as strongly antigenic. Digestion analysis verified the safety and stability of the peptides predicted during this study. Furthermore, docking analyses of predicted peptides showed significant interactions with the HLA-B7 allele.Conclusion: The putative antigen epitopes identified in this study may serve as vaccine candidates and can help to eliminate/control growing health threat of COVID-19.
"Prevalence of role stressors and associated factors among health surveillance assistants in Malawi? A cross-sectional study"
Authors: Ntopi Simon , Chirwa Ellen , Maluwa Alfred 
Published: February 2020

Abstract
Background: Health Surveillance Assistants (HSAs) are community-based health workers in Malawi, responsible for health services delivery at the community level. The prevalence of high role stressors among health surveillance assistants (HSAs) is a very important issue to strengthen the delivery of the essential health package at the community level; however, data on the prevalence of these role stressors to help understand more about the role stressors among HSAs is unavailable. This study aimed to investigate the prevalence of role stressors and its associated factors among HSAs. Methods: A cross-sectional study design was used in this study to collect data from January 2017 to December 2017. Data were collected from 430 HSAs from the districts of Mangochi, Lilongwe and Mzimba using a self-administered structured questionnaire was hand delivered to a total of 455 HSAs (response rate of 94.5%). Role ambiguity and role conflict was measured using a 13-item Rizzo et al. role conflict and ambiguity (RCA) scale; role overload was measured using a 9-item Reilly role overload scale and the job tasks for HSAs were measured using Burgal et al. modified version of the task inventory scale. Factors for role stressors were analyzed using the principal component analysis (PCA) and multiple linear regression model. Results: Of the respondents 3% had high risk for RA, 51.20% RC and 49% RO. Role stressors are prevalent among HSAs in Malawi. HSAs who were resident in rural areas, female, and performing additional clinical roles experienced significant levels of role stressors. Interestingly, no statistically significant associations were found between role stressors and the years at service post, age and the district of work. Conclusion: This is the first study of occupational role stressors in Malawi. Role stressors are prevalent among HSAs. Interventions aimed at improving supervision, training and task regulation by an independent regulatory body may support to reduce role stressors among HSAs. Keywords : health surveillance assistants, role ambiguity, role conflict, role overload, role stressors
A quantified discrimination of a query compound between target classes under 3D-similarity metric
Authors: Lee Sanghyeok , Ahn Sangjin , Kim Mi-hyun 
Published: February 2020

Abstract
Background: 3D similarity is useful to predict the profiles of unprecedented molecular frameworks, 2D dissimilar to known compounds. Basically, when comparing compound pairs, 3D similarity of the pairs depends on conformational sampling of compounds, alignment method, chosen descriptors, and metric to show limited discriminative power. In addition to four factors, 3D chemocentric target prediction of an unknown compound requires compound - target associations. The associations for the target prediction replace compound-to-compound comparison with compound-to-target comparison. Results: Quantitative comparison of query compounds to target classes (one-to-group) could be acquired using two type similarity distributions: one is from maximum likelihood (ML) estimation of queries and another is from Gaussian mixture model (GMM) of target classes. While Jaccard-Tanimoto similarity of query-to-ligand pairs could be transformed into query distribution through ML estimation, the similarity of ligand pairs within each target class could be transformed into the representative distribution of a target class through GMM, hyperparameterized through expectation-maximization (EM) algorithm. To quantify the discriminativeness of a query ligand against target classes, Kullback-Leibler (K-L) divergence was calculated between two distributions.Conclusions: Stratified sampled 14K ligands from four target classes, estrogen receptor alpha (ESR), vitamin D receptor (VDR), cyclooxygenase-2 (COX2), and cathepsin D (CTSD) presented whether or not each query can be a representative ligand of each target through compared K-L divergence value. The feasibility index, Fm and the probability, from K-L divergence could summarize 3D chemocentric relationship between target classes.
A split supply of bio-organic and chemical fertilizer synergistically affects root system architecture and improves above-ground growth in pear tree (Pyrus pyrifolia Nakai)
Authors: Kang Yalong , An Xiangrui , Ma Yanwei , Li Yan , Wu Wenli , Dong Caixia , Xu Yangchun , Shen Qirong 
Published: February 2020

Abstract
Background: Root system architecture (RSA) is highly plastic, responding to nutrient availability and the heterogeneity of the soil environment. However, the linkage of root morphology to anatomy and root nutrient, and its implication for root function at the heterogeneous application of bio-organic and chemical fertilizer have not yet been defined, especially for pear trees.Results: In this study, a split-root experiment was conducted using 1-year old ‘Cuiguan’ trees. No fertilizer (NF), chemical fertilizer (CF), and bio-organic fertilizer (BIO) were paired to test six combinations: NF-NF, NF-CF, NF-BIO, CF-CF, BIO-BIO, and BIO-CF. Root morphological, anatomical traits and root nutrient concentrations, and their relationships were determined. Trees receiving BIO had significantly higher lateral root numbers, activity, total lateral root length, and specific root length than trees receiving no BIO, while the effects on root tissue density were the direct opposite. Compared with CF-CF treatment, root xylem thickness, stele diameter, vessel diameter, and number of vessels all increased in response to BIO-CF treatment. Root growth was synergistically promoted in BIO-CF, with increased special root length and root nitrogen concentration, but root tissue density and the carbon:nitrogen ratio were reduced. Intriguingly, the synergistic effect resulted in greater trunk girth without sacrificing height, compared to trees receiving CF or BIO alone.Conclusions: The combination of BIO and CF improves root traits and tree growth, suggesting that using bio-organic fertilizer as a supplement to reduce the application rate of chemical fertilizer is beneficial to orchard ecosystems.
Exploring the Role of Asp1116 in Selective Drug Targeting of CREBcAMP- Responsive Element-binding Protein Implicated in Prostate Cancer
Authors: Akinsiku Oluwayimika E. , Soremekun Opeyemi S. , Olotu Fisayo A. , Soliman Mahmoud E.S. 
Published: February 2020

Background:
The selective targeting of CREB-cAMP-responsive element-binding
protein (CBP) has recently evolved as a vital therapeutic approach for curtailing its aberrant
upregulation associated with the development of prostate cancer. Inhibition of CBP has been
discovered to be an important therapeutic option in androgen receptor signalling pathway mediated
prostate cancer. Y08197, a novel selective inhibitor of CBP, has shown promising therapeutic
outcome in prostate carcinogenesis over non-selective analogues such as CPI-637.

Methods/Results:
Herein, we used molecular dynamics simulation to gain insights into the
mechanistic and selective targeting of Y08197 at the bromodomain active site. Molecular
Mechanics/ Poisson-Boltzmann Surface Area (MM/PBSA) analysis revealed a similar inhibitory
effect between Y08197 and CPI-637. Furthermore, in exploring the selective affinity of Y08197
towards CBP in combination with Bromodomain and PHD finger-containing protein 1(BRPF1),
our findings highlighted Asp1116 as the ‘culprit’ residue responsible for this selective targeting.
Upon binding, Asp1116 assumed a conformation that altered the architecture of the bromodomain
active site, thereby orienting the helices around the active site in a more compacted position. In
addition to some specific structural perturbations mediated by Asp1116 on the dynamics of CBP,
our study revealed that the strong hydrogen bond interaction (N-H...O) elicited in CBP-Y08197
sequestered Y08197 tightly into the CBP bromodomain active site.

Conclusion:
Conclusively, the inhibition and selective pattern of Y08197 can be replicated in
future structure-based CBP inhibitors and other bromodomain implicated in carcinogenesis.
The Prevalence of Streptococcus Mutans with Different ABO Blood Groups Among Healthy College Students
Authors: Al-Mahmood Sumayah , Al-Kaisy Aedah Z. , Mahmood Maha A. , Aldhaher Zainab A. , Sabri Abbas 
Published: February 2020

Objective:This study was conducted to determine the correlation between the occurrence ofStreptococcus mutans(S. mutans) which is considered as the main pathogen responsible for the initiation and development of dental caries with blood groups and Rhesus (Rh) factor that are considered as a genetic predictor of having dental caries among healthy students.Methods:Saliva and blood samples were taken from 40 healthy students whose ages were between 19-23 years old in the College of Dentistry/Al-Iraqia University in Baghdad, Iraq. Estimation of the flow rate and pH was done for all the collected saliva samples within 5 min of saliva collection. Then, 100 µL of saliva was cultured on Mitis salivarius bacitracin agar (MSBA) at 37 °C for 48 h. The colonies ofS. mutanswere identified by their morphology and biochemical tests. Later, 1 to 2 drops of blood were taken from each student’s thumb to determine the blood group.Results:This study showed there was a greater prevalence ofS. mutansamong students of blood group A in contrast to the blood group O. In addition, Rh+ groups were dominant than Rh- groups among the study samples.Conclusion:S. mutanslevels were higher in the blood group A followed by AB, B, O blood groups among the college students studied.
An Efficient Clustering-Based Segmentation Approach for Biometric Image
Authors: Shukla Aparna , Kanungo Suvendu 
Published: February 2020

Background:
Image analysis plays a vital role in the biometric identification system. To
achieve the effective outcome of any biometric identification system, the inputted biometric image
taken should be of fine quality as it greatly impacts the decision. Image segmentation is a significant
aspect of image analysis that must be carried out for enhancing the quality of an image. It efficiently
differentiates the foreground and background region of the inputted biometric image and
facilitates further image processing simply by providing a segmented binary image which is more
coherent to the system.

Objective:
We present an efficient clustering based image segmentation approach to obtain the quality segmented binary image that further processed to get the quality decision in biometric based identification system.

Method:
A centre of mass based centroid clustering approach for image segmentation has been proposed to perform binarization of an image so as the adequate and operative results can be found.

Result:
The performance of the proposed approach has been applied on different sets of biometric data set having different number of hand images. Resultant this approach provides the sharp and lucid images so that good enough and effective intended results can be obtained.

Conclusion:
The centroid based clustering approach for image segmentation outperform the existing clustering approach. In order to measure the quality of segmented binary image three statistical performance parameters are used: PSNR, SUMD and Time Elapsed (sec).
Retóricas de la distinción en la crónica latinoamericana
Authors: Viu Adagio Julieta 
Published: February 2020

Este artículo se propone trazar un mapa de la crónica latinoamericana moderna e iluminar una serie de hitos artísticos y culturales que signaron su historia. Con el objetivo de identificar y periodizar los intereses hegemónicos de los escritores en relación con el campo cultural y las lecturas teóricas generales del arte y la literatura. Estos análisis permitieron identificar el auge del divismo modernista caracterizado, entre otras cuestiones, por la reivindicación de la aristocracia del arte; la celebridad basada en una retórica de la fama; y en oposición a éstos la emergencia de la plebeyez que ubica en el centro de la representación a personajes populares. La originalidad de este trabajo reside en la lectura del campo de la crónica cuya tradición de estudios en general ha avanzado por autores apreciados individualmente. De igual importancia en cuanto a su originalidad es el haber identificado los tópicos descritos, desconsiderados por el campo de la crítica. Abstract: This article aims to draw a map of the modern Latin American chronicle and illuminate a series of artistic and cultural milestones that marked its history. With the aim of identifying and periodizing the hegemonic interests of writers in relation to the cultural field and the general theoretical readings of art and literature. These analyzes allowed us to identify the rise of the modernist divism characterized, among other issues, by the claim of the aristocracy of art; the celebrity based on a rhetoric of fame; and in opposition to these the emergence of the plebeyez that locates popular characters in the center of the representation. The originality of this work lies in the reading of the field of the chronicle whose tradition of studies in general has advanced by authors appreciated individually. Of equal importance in the originality of this paper is the identification of the topics described, disregarded by the field of criticism.Key words: Latin American Chronicle; Modernism; The Famous; The Plebeian.
Ketentuan Calon Kepala Daerah Dari Pns Menurut Undang-Undang Nomor 5 Tahun 2014 Tentang Aparatur Sipil Negara
Authors: Syuib Muhammad 
Published: February 2020

Undang-Undang Dasar 1945 mengisyaratkan bahwa setiap warga negara memiliki hak yang sama untuk mencalonkan diri sebagai calon kepala daerah. Isyarat ini tertuang dalam Pasal 28D ayat (3) yang menyebutkan bahwa “Setiap warga negara berhak memperoleh kesempatan yang sama dalam pemerintahan”. Berdasarkan Pasal 123 ayat 3 Undang-Undang Nomor 5 Tahun 2014 tentang Aparatur Sipil Negara menyatakan bahwa Pegawai ASN dari PNS yang mencalonkan diri atau dicalonkan menjadi gubernur dan wakil gubernur; bupati/walikota dan wakil bupati/wakil walikota wajib menyatakan pengunduran diri secara tertulis sebagai PNS sejak mendaftar sebagai calon. Untuk itu, dibutuhkan penelitian lebih lanjut untuk mengetahui landasan pertimbangan PNS harus mundur jika maju dalam Pilkada. Maka dari itu, penelitian ini bertujuan untuk menemukan dan menjelaskan landasan pertimbangan sehingga PNS harus mundur dari jabatan organiknya jika maju sebagai calon kepala daerah. Metode penelitian yang digunakan adalah yuridis normatif yang menitik beratkan pada penelitian data kepustakaan atau yang disebut data sekunder, serta mengkaji peraturan perundang-undangan berkaitan dengan persyaratan pencalonan bagi PNS yang maju sebagai calon kepala daerah. Hasil penelitian menunjukkan bahwa PNS harus mundur dari jabatan negeri adalah bagian dari ketundukkannya terhadap aturan-aturan yang mengatur birokrasi pemerintahan dan aturan-aturan kepegawaian karena ketika seseorang telah memilih menjadi PNS maka dia telah mengikatkan diri dalam ketentuan-ketentuan yang mengatur dirinya sebagai aparatur negara. Selain itu, agar terhindar dari adanya penyalahgunaan wewenang karena hal itu melanggar hukum.
Itinerários filosóficos da Ciência da Informação no Brasil: o pioneirismo do IBICT e a propagação das ideias
Authors: Pinheiro Lena Vania Ribeiro 
Published: February 2020

Análise, sob a abordagem da epistemologia histórica, do surgimento, desenvolvimento e estágio atual de pesquisas fundamentadas ou com foco em Filosofia, na Ciência da Informação e seu objeto, relacionando-as a pesquisadores, fatos, eventos e influências nacionais e do exterior. O mestrado em Ciência da Informação do IBICT, introdutor dos estudos filosóficos, é traçado na sua trajetória acadêmica, bem como os demais Programas, nas suas áreas de concentração e linhas de pesquisa, para verificação da presença de estudos filosóficos. A análise incluiu, ainda, as instituições e eventos que propiciaram o desenvolvimento de pesquisas na área, como a ANCIB e os ENANCIB. A pesquisa é finalizada por uma análise da influência do pensamento de Rafael Capurro na produção científica deste campo do conhecimento, evidenciada por análise de citação nos ENANCIB. Os resultados demonstram a expansão de estudos teóricos, o seu aprofundamento e, consequentemente, maior densidade teórica no estatuto científico da Ciência da Informação.
Memórias e histórias do departamento de bibliotecas escolares e comunitárias de Florianópolis: 1988 a 2018
Authors: Capistrano Tatiana Quadra e Silva , Unglaub Tânia Regina da Rocha 
Published: February 2020

A memória está relacionada com a construção de armazenamentos de informações e fatos vivenciados. Entende-se que ela é a matéria prima da história, e é considerada um dos alicerces que dá sentido à vida, a sociedade e a uma instituição. Dentre as classificações de estudos sobre memória há a memória institucional, que tem como objetivo manter viva e pública a trajetória de instituições, por meio de relatos, fotos, documentos e/ou objetos, que de forma organizada e com informações consistentes transmitem a história do local. Com essa concepção, este artigo apresenta a origem e trajetória da atuação do Departamento de Bibliotecas Escolares e Comunitárias (DEBEC) da rede municipal de Florianópolis, que engloba os anos de 1988 a 2018. Esse estudo faz parte de uma pesquisa de mestrado profissional em andamento, vinculada ao Programa de Pós-Graduação em Gestão da Informação da Universidade do Estado de Santa Catarina. Trata-se de uma pesquisa histórica, desenvolvida por estudos bibliográficos, exame a fontes documentais e entrevistas a protagonistas que fizeram parte dessa história. Assim como tece uma colcha de retalhos, essa pesquisa foi construída. Um depoimento aqui e outro documento acolá encontraram e teceram a trajetória histórica dessa instituição. A temática surgiu devido a aproximação da pesquisadora com as atividades e projetos criados e desenvolvidos por esse Departamento junto aos bibliotecários da rede. Os resultados obtidos contribuíram para de suprir a lacuna sobre a memória institucional do Departamento e produzir reflexões sobre a importância desse Departamento para a Rede de Bibliotecas Escolares e Comunitárias de Florianópolis.
O buraco negro criado pelas Tecnologias da Informação e Comunicação
Authors: Carvalho Antonio Aparecido de , Farina Milton Carlos , Carvalho Leonardo Birche de 
Published: February 2020

A pesquisa teve o intuito de trazer à tona uma análise crítica acerca da forma de coleta, armazenamento e acesso a dados e informações, anteriormente documentados eram arquivados em meio manual, tais como relatórios, livros dentre outros, já na atualidade a tecnologia de informação e comunicação trouxe a ideia de que as informações devem estar armazenadas em meios digitais, nuvens, pen drives, e-mails e demais hardwares, contudo surge o seguinte questionamento: “O que nos garante que no futuro teremos programas, dispositivos e ferramentas compatíveis para acessá-las?” Documentos e informações anteriormente armazenados em fitas cassetes, disquetes ou nas memórias de computadores antigos, por vezes se tornam inacessíveis devido à indisponibilidade de dispositivos devido à obsolescência de equipamentos ou até mesmo das constantes atualizações de softwares. A impossibilidade de acesso às informações arquivadas digitalmente é conhecida como “Buraco Negro Digital”. A pesquisa demonstra que algumas organizações estão antevendo problemas e buscando possíveis soluções para preservação documental e consequentemente da nossa história para as gerações futuras, contudo muito há de ser feito.
The Potential Wind Power Resource in Central-South of the Constanta County
Authors: Boboc Emilian 
Published: February 2020

Abstract
Usually, wind turbine generator’s structures or radio masts are located in wind exposed sites. The paper aims to investigate the wind conditions in the nearby area of Cobadin Commune, Constanta County, Romania at heights of 150-200m above the surface using global reanalysis data sets CFSR, ERA 5, ERA I and MERRA 2.
Using the extreme value theory and the physical models of the datasets, the research focuses on the assessment of the maximum values that are expected for the wind speeds, but the wind statistics created can be used for a further wind or energy yield calculation.
Without reaching the survival wind speed for wind turbine generators, with mean wind speed values higher than 7 m/s and considering the cut-in and cut-out wind speeds of 3 m/s, respectively 25 m/s, the site can be exploited in more than 90% of the time to generate electricity, thus, the paper is addressed to the investors in the energy of renewable sources. At the same time, the insights of the wind characteristics and the knowledge of the extreme values of the wind speed can be useful, not just for the designers, in the rational assessment of the structural safety of wind turbines, but also those evaluating the insured losses.