bluuebunny/crossref_metadata_2025_split huggingface dataset:
The etiology and prognosis of canalicular laceration repair using canalicular anastomosis combined with bicanalicular stent intubation
Authors: Wang Hongwei , Qin Xiuhong , Ji Jiali , Lu Yang , Xu Li , Guo Tao , Xiao Caiwen , Zhang Zhenzhen 
Published: February 2020

Abstract
Background: To evaluate the etiology of lacrimal canalicular laceration and explore possible risk factors influencing prognosis. Methods: A total of 142 patients (142 eyes) with lacrimal canalicular lacerations were reviewed and surgically repaired using canalicular anastomosis combined with bicanalicular stent intubation between March 2017 and March 2018. The analyzed data contained demographic information, the types of trauma, injury locations, associated additional ocular injuries and surgerical outcomes at follow-up. The main outcome measures were anatomic success, functional success, and the complications of surgery. Results: The mean patient age was 42.07years(ranging from 1-75 years) and 112 (78.87%) were men. Upper and lower canalicular lacerations were found in 14 (9.86%) and 112 patients (78.87%), respectively, and both canalicular lacerations were found in 16 patients (11.27%). Electric bike accidents were the leading cause of injury with 76 patients (53.52%). There were 100 (70.42%) patients who had lid laceration without tarsal plate fracture and 42 (29.58%) patients who had lid laceration with tarsal plate fracture. Anatomic success rate was 98.59%, and functional success rate was 83.8%. Functional reconstruction failure rates were higher in patients with indirect injuries, lid laceration with tarsal plate fracture, and with punctum splitting( P <0.05). Surgical Complications were detected in the form of lacrimal punctum ectropion in 3 (2.11%) patients, punctum splitting in 2 (1.41%) patients, stent extrusion and loss in 2 (1.41%) patients. Conclusions: Electric bike accidents have become the leading cause of injury instead of the motor vehicle accidents because of changes in lifestyle. The indirect injuries, lid laceration with tarsal plate fracture and with punctum splitting, were significantly more likely to lead to a poor prognosis, as comfirmed by the lower functional success rate of surgery.
Risk factors for progression of solitary plasmacytoma of bone to multiple myeloma in the spine: A population-based study
Authors: Xie Lin , Wang Hongli , Jiang Jianyuan , Zheng Chaojun 
Published: February 2020

Abstract
Background: Spine SBP is a primary spinal malignant tumor. Risk factors associated with progression of solitary plasmacytoma of bone (SBP) to multiple myeloma in spine remains unknown. This study therefore aimed to identify the risk factors associated with progression of solitary plasmacytoma of bone (SBP) to multiple myeloma in the spine.Methods: Data of 1543 patients diagnosed with spine SBP between 1992 and 2013 was obtained from the Surveillance, Epidemiology, and End Results (SEER) database for analysis. Risk factors associated with progression of SBP to multiple myeloma (MM) were then identified using univariate and multivariate regression analysis methods.Results: Out of the 1543 patients diagnosed with spine SBP, 659 of them progressed to MM. The overall rate of progression to MM was 42.51%. Univariate and multivariate regression analysis revealed that age, race, gender and chemotherapy were independent risk factors associated with SBP progression to MM.Conclusion: Old aged patients, patients belonging to the white race, female patients and those undergoing chemotherapy were identified to be at a high risk of progression to MM. This information will assist clinicians in evaluating patients’ risk of SBP progression to MM at the point of diagnosis and advise them accordingly.
Characterization of astrocytes throughout life in wildtype and APP/PS1 mice after early-life stress exposure
Authors: Abbink Maralinde R. , Kotah Janssen M. , Hoeijmakers Lianne , Mak Aline , Yvon-Durocher Genevieve , Gaag Bram van der , Lucassen Paul J. , Korosi Aniko 
Published: February 2020

Abstract
Background Early-life stress (ES) is an emerging risk factor for later-life development of Alzheimer’s disease (AD). We have previously shown that ES modulates amyloid-beta pathology and the microglial response to it in the APPswe/PS1dE9 mouse model. Because astrocytes are key players in the pathogenesis of AD, we studied here if and how ES affects astrocytes in wildtype (WT) and APP/PS1 mice, and how these relate to the previously reported amyloid pathology and microglial profile. Methods We induced ES by limiting nesting and bedding material from postnatal days (P) 2-9. We studied in WT mice (at P9, P30 and 6 months) and in APP/PS1 mice (at 4 and 10 months) i) GFAP coverage, cell density and complexity in hippocampus (HPC) and entorhinal cortex (EC); ii) hippocampal gene expression of astrocyte markers; and iii) the relationship between astrocyte, microglia and amyloid markers. Results In WT mice, ES increased GFAP coverage in HPC subregions at P9, and decreased it at 10 months. APP/PS1 mice at 10 months exhibited both individual cell as well as clustered GFAP signals. APP/PS1 mice when compared to WT exhibited reduced total GFAP coverage in HPC, which is increased in the EC, while coverage of the clustered GFAP signal in the HPC was increased and accompanied by increased expression of several astrocytic genes. While measured astrocytic parameters in APP/PS1 mice appear not be further modulated by ES, analyzing these in the context of ES-induced alterations to amyloid pathology and microglial shows alterations at both 4 and 10 months of age. Conclusions Our data suggest that ES leads to alterations to the astrocytic response to amyloid-β pathology.
Tissue Adhesive for Wound Closure in enhanced-recovery Total Hip Arthroplasty: A Prospective, Randomized and Controlled Study
Authors: Kong Xiangpeng , Yang Minzhi , Cao Zheng , Chen Jiying , Chai Wei , Wang Yan 
Published: February 2020

Abstract
Background: We aimed to present our experience of adopting tissue adhesive as adjunct to standard wound closure in total hip arthroplasty (THA) and evaluate its performance.Methods: From September 2019 to November 2019, we prospectively enrolled consecutive patients who underwent simultaneous bilateral THA in this randomized and controlled study. Standard wound closure was applied on one side of hip while additional tissue adhesive was applied on the other side at random. We collected and analyzed patients’ information, including age, gender, body mass index (BMI), diagnosis, postoperative length of stay (LOS), dressing changes, wound evaluation scores, wound-related cost and complications.
Results: Thirty patients with simultaneous bilateral THA were enrolled in this study. During the hospital stay, the times of dressing change in hips with tissue adhesive was significantly less than that in the other hips (p=0.000). However, the wound-related cost in hips with tissue adhesive was significantly higher (p=0.000). According to patients’ feedback at one-month follow-up, wound evaluation of hips with tissue adhesive was significantly better than the other hips (p=0.004). Seventeen patients preferred tissue adhesive and only five patients preferred standard wound closure.
Conclusions: Tissue adhesive could significantly reduce wound drainage and increase patients’ satisfaction, which can be an ideal adjunct to standard wound closure in enhanced-recovery THA.
ALDH1A3 serves as a predictor for castration resistance in prostate cancer patients
Authors: Wang Shangqian , Zhou Xiang , Liang Chao , Bao Meiling , Tian Ye , Zhu Jundong , Zhang Tongtong , yang jie , Wang Zengjun 
Published: February 2020

Abstract
Aldehyde dehydrogenase 1A3 (ALDH1A3) has been implicated in the survival and proliferation of prostate cancer cells. We retrospectively reviewed our patients with advanced disease on adjuvant hormonal therapy after prostatectomy. Time to castration resistance stage was documented. And Immunohistochemistry analysis for ALDH1A3 was performed for those patient samples on tissue microarray. Using bioinformatics analysis for RNA sequencing data of both primary prostate cancer and metastatic castration resistance prostate cancer (mCRPC) from online datasets, we have found that the expression level of ALDH1A3 is lower in mCRPC group than in primary cancer group. Crispr-Cas9 was used to knock out ALDH1A3 in prostate cancer luminal cells, and morphologic analysis as well as the Gene Set Enrichment Analysis (GSEA) were facilitated to discover the mechanisms of the resistance phenotype. We found that the patients with ALDH1A3 low expression had shorter time to progression to castration resistance compared with those of higher expression group on adjuvant hormonal therapy after radical prostatectomy. The ALDH1A3 knockout cells gradually acquired resistance to androgen deprivation therapy, a few cells have been found in knockout group showing as that the spindle-like luminal cells in charcoal stripped medium. Furthermore, PI3K pathway activation has been confirmed by Western blot. The PI3K pathway inhibitor BEZ235 has been demonstrated that the acquired ADT resistance by ALDH1A3 down regulation could be rescued by PI3K pathway inhibitor. Together, these results suggested a novel function for ALDH1A3 in development of mCRPC, and indicated PI3K pathway inhibitor has the potential in the treatment of a subgroup of mCRPC patients.
Association between Epstein-Barr Virus Infection and Gastric Cancer: A Systematic Review and Meta-analysis
Authors: Tavakoli Ahmad , Monavari Seyed Hamidreza , Mohammadi Farid Solaymani , Kiani Seyed Jalal , Armat Saber , Farahmand Mohammad 
Published: February 2020

Abstract
Background: Numerous studies conducted over the past 30 years have pointed to the presence of Epstein–Barr virus (EBV) in gastric cancer samples. This study was aimed to provide a meta-analytic review of the prevalence of EBV in gastric cancer patients, and to clarify the relationship between EBV infection and gastric cancer. Methods: A literature search was performed electronically using online databases for English language publications until July 1, 2019. The pooled EBV prevalence and 95% confidence intervals (CIs) were estimated using a random effects model. To determine the association between EBV and gastric cancer, pooled odds ratio (OR) and its 95% CI were computed for case-control studies with matched pairs design. Results: The pooled prevalence of EBV in 20411 gastric cancer patients was 8.78% (95% CI: 7.75-9.93%; I 2 =83.0%). The proportion of EBV-associated gastric cancer among male cases was significantly higher than among female cases (10.85%, vs 5.72%) ( P <0.01). EBV was more prevalent in the cardia (12.47%) and in the body (11.68%) compared to the antrum (6.29%) ( P <0.01). There were 20 studies with matched pairs design, including tumor and tumor-adjacent normal tissue pairs from 4116 gastric cancer patients. The pooled OR between EBV infection and gastric cancer risk was 18.56 (95% CI: 15.68–21.97; I 2 = 55.4%). Conclusion: EBV infection is associated with more than 18 times increase risk of gastric cancer. Although the prevalence of EBV was higher in male patients than in female patients with gastric cancer, women are more likely than men to develop EBV-associated gastric cancer.
A comprehensive study on the oncogenic mutation and molecular pathology in Chinese lung adenocarcinoma patients
Authors: Zhang Xilin , Jiang Yan , Yu Huanming , Xia Hui , Wang Xiang 
Published: February 2020

Abstract
Background: Several driver genetic alterations have been identified in micropapillary lung adenocarcinoma (MPA). However, the frequency of ROS1 rearrangements is yet unclear. Herein, we investigated the associations between clinicopathological and molecular characteristics of MPA compared with non-micropapillary lung adenocarcinoma (LA).Methods: FFPE sections derived from lung adenocarcinoma patients who never received adjuvant chemotherapy or radiation therapy prior to surgical resection were collected from October 2016 to June 2019. EGFR mutations, ROS1 rearrangements and EML4-ALK fusions were identified in a set of 131 MPA and LA cases by using the Amplification Refractory Mutation System.Results: EGFR mutations had occurred in 42 (76.4%) MPA patients and 42 (55.3%) LA patients. But interestingly, ROS1 rearrangement was present in 10.9% (6/55) MPA cases and 1.3% (1/76) LA cases. Moreover, 7.3% (4/55) MPA samples had multiple gene mutations, while only 1.3% (1/76) LA cases had double gene alterations.Conclusions: A higher prevalence of ROS1 rearrangements or combined mutations of ROS1 and EGFR or EML4-ALK may play a critical role in the tumorigenesis of MPA. These finding provides a novel therapy strategy for the patients with malignant MPA through combining TKIs than one TKI.
Identification of blast resistance QTLs based on two advanced backcross populations in rice
Authors: Jiang Haichao , Feng Yutao , Qiu Lei , Gao Guanjun , Zhang Qinglu , He Yuqing 
Published: February 2020

Abstract
Background: Rice blast is an economically important and mutable disease of rice. Using host resistance gene to breed resistant varieties has been proven to be the most effective and economical method to control rice blast and new resistance genes or quantitative trait loci (QTLs) are then needed. Results: In this study, we constructed two advanced backcross population to mapping blast resistance QTLs. CR071 and QingGuAi were as the donor parent to establish two BC 3 F 1 and derived BC 3 F 2 backcross populations in the Jin23B background. By challenging the two populations with natural infection in 2011 and 2012, 16 and 13 blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi population, respectively. Among Jin23B/CR071 population, 3 major and 13 minor QTLs have explained the phenotypic variation from 3.50% to 34.08% during six observation times. And, among Jin23B/QingGuAi population, 2 major and 11 minor QTLs have explained the phenotypic variation from 2.42% to 28.95% during six observation times. Conclusions: Sixteen and thirteen blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi population, respectively. QTL effect analyses suggested that major and minor QTLs interaction is the genetic basis for durable blast resistance in rice variety CR071 and QingGuAi.
Derangements in Coronary Calcium Score and Left Ventricular Global Longitudinal Strain in Patients Post Left Breast Radiotherapy
Authors: Okwuosa Tochi , Palomo Andres , Dangana Thelma , Yakupovich Anel , Basu Sanjib , Fughhi Ibtihaj 
Published: February 2020

Abstract
Background Radiotherapy is a common treatment for breast cancer, with unintended long-term CV consequences; and no consensus on adequate CV screening methods to prevent future events. We aimed to assess the use of coronary artery calcium (CAC) and left ventricular global longitudinal strain (GLS) to identify those at risk for CVD due to radiotherapy. Methods Using data from an urban cancer registry, we enrolled 17 women (mean age 62 years) with left-sided breast cancer from 1999-2003, treated with radiotherapy. Agatston CAC score was measured by CT scan; and GLS by echocardiography. Logistic regression was used to compare study CAC scores with historical controls using age- and race-matched (Multi-Ethnic Study of Atherosclerosis) MESA CAC calculator [derived from MESA cohort from 6 US communities, free of clinical CVD at baseline (2000-2002) https://www.mesa-nhlbi.org ]; and GLS with historical controls from prior meta-analysis with defined normal GLS values ( Yingchoncharoen et al). Results The Hosmer-Lemeshow goodness of fit test reported a significant lack of fit (p < 0.02) compared with the expected probability of non-zero CAC in the historical controls from MESA. The mean GLS of -20.6% (95% CI: -21.50%, -19.79%), and was not statistically different when compared with the average of the historical controls. However, there was a significant difference of GLS compared to the meta-analysis using a one-sample T test with P=0.03. Conclusion We found that women post left sided breast radiotherapy had higher CAC presence without significant difference in mean GLS, compared with historical controls from the MESA database and prior GLS meta-analysis.
Antiobesity activity of Acer tegmentosum Maxim on 3T3-L1 preadipocyte and high-fat diet-induced obese rats
Authors: Cho Hang-Hee , Lee Soo-Jung , Kim Sung-Ho , Jang Sun-Hee , Won Chungkil , Kim Hong-Duck , Kim Tae Hoon , Cho Jae-Hyeon 
Published: February 2020

Abstract
Background: The aim of this study was to investigate the effect of Acer tegmentosum Maxim (ATM) on adipocyte differentiation in 3T3-L1 adipocyte-derived cells and anti-obesity properties in high fat diet (HFD)-induced obese rats.

Methods: 3T3-L1 adipocytes and HFD-induced obese rats were treated with ATM, and its effect on gene expression was analyzed using RT-PCR and Western blotting experiments.

Results: Cellular lipid contents in DMI (dexamethasone, 3-isobutyl-1-methylxanthine, and insulin mixture)-treated cells increased, while ATM treatment caused a significant reduction in lipid accumulation in differentiated 3T3-L1 cells. ATM caused inhibition of adipogenesis via down-regulation of the CCAAT/enhancer binding protein β (C/EBPβ), C/EBPα, and peroxisome proliferator-activated receptor γ (PPARγ) expressions in 3T3-L1 cells. Moreover, treatment with ATM caused a decrease in the expressions of adipocyte-specific genes, such as adipocyte fatty acid-binding protein-2 (aP2), fatty acid synthase (FAS), and lipoprotein lipase (LPL), compared with DMI-stimulated adipocytes. In addition, phosphorylation levels of protein kinase B (Akt) and its downstream substrate, glycogen synthase kinase 3β (GSK3β), were significantly decreased by ATM treatment of 3T3-L1 adipocytes. Together, these results indicated that ATM caused inhibition of both adipocyte differentiation via suppression of the C/EBP family and PPARγ expressions and the Akt signaling pathway in 3T3-L1 adipocytes. In the present study, we further investigated anti-obesity effects of ATM on HFD-induced obese rats. Rats fed with HFD demonstrated elevations in body weight gain, while the administration of ATM significantly reversed BW gains and adipose tissue weights in rats fed HFD. ATM supplementation also caused a decrease in the circulating triglyceride levels and total cholesterol levels and led to inhibition of lipid accumulation in the adipose tissues in HFD-induced obesity in rats. Furthermore, epididymal fat exhibited larger adipocytes in the HFD group, whereas the ATM-treated group was significantly smaller than that of HFD group. These results strongly demonstrate that ATM administration caused a reduction in adiposity via attenuation in adipose tissue mass and adipocyte size.

Conclusion: These finding demonstrated that ATM exerted anti-obesity effects through inhibition of adipocyte differentiation and adipogenesis, leading to a decrease in BW and fat tissue mass in HFD-induced obesity in rats.
A novel COMP Mutation in a Chinese Family with Multiple Epiphyseal Dysplasia
Authors: Shao Jiashen , Zhao Sen , Yan Zihui , Wang Lianlei , Zhang Yuanqiang , Lin Mao , Yu Chenxi , Wang Shengru , Niu Yuchen , Li Xiaoxin , Qiu Guixing , Zhang Jianguo , Wu Zhihong , Wu Nan 
Published: February 2020

Abstract
Background: Multiple Epiphyseal Dysplasia (MED) is a skeletal disorder characterized by delayed and irregular ossification of the epiphyses and early-onset osteoarthritis, at least 66% of the reported autosomal dominant MED (AD-MED) cases are caused by COMP mutations. Methods : Here, we reported a four-generation Chinese family with early-onset hip osteoarthritis, flatfoot, brachydactyly, and mild short stature. Whole exome sequencing was performed on the proband followed by enquiring family history, detailed physical examination, and radiographic evaluation. After that, the pathogenicity of candidate mutation was also analyzed. Results : We recruited a dominant MED family with 10 affected members and 17 unaffected members. The main radiographic findings were symmetric changes in dysplastic acetabulum and femoral head, irregular contours of epiphyses, shorted femoral neck, and flatfoot. Lower bone density of ankle joint , wrist joint, and knees were observed as well as irregular vertebral end plates. As a result, we found a missense mutation c.1153G>T (p. Asp385Tyr) located in the exon 11 of COMP gene. This mutation was assessed as “pathogenic” for the low allele frequency and a high likelihood of co-segregation with disease in the reported family. Sanger sequencing identified a novel heterozygous mutation c.1153G>T (p. Asp385Tyr), in exon 11 of COMP gene in all affected male individuals. Conclusions: Our result underlined the key role for Asp385 amino acid in the protein function of COMP, and first confirmed the pathogenicity of COMP (c.1153G>T; p. Asp385Tyr) in AD-MED disease. We expanded the mutational spectrum of COMP and phenotypic information of AD-MED.
PSORS1C1 gene polymorphism is associated with autoimmune thyroid diseases in the Chinese population
Authors: Author) Yanfei Jiang(Former Corresponding , Liu Zhiwen , Song Ronghua , Mu Kaida , Yang Yanping , Zhang Jinan 
Published: February 2020

Abstract
Objective Autoimmune thyroid disease(AITD) is not only an immune-related disorder, but also inherited as a complex gene-related disease. The psoriasis susceptibility 1 candidate 1(PSORS1C1) gene is a susceptibility locusin autoimmune diseases. Many autoimmune diseases have been proved to be related with the PSORS1C1 gene. However, whether the PSORS1C1 gene plays a role in affecting the predisposition to AITD remains unknown.Methods In this study, we included 1056 Chinese Han AITD patients and 954 matched healthy controls. Multiplex polymerase chain reaction (PCR) technology was used to genotype SNPs (rs3130983, rs3778638, rs3815087, rs4959053)in PSORS1C1 gene. Results An association between rs3778683 and Graves' disease (GD) was observed with statistical significance (p=0.039). The allele and genotype of single nucleotide polymorphism (SNP) rs3130983, rs3815087 and rs4959053 showed no significant association with AITDs. In addition, no linkage disequilibrium for rs3130983, rs3815087, rs3778638 and rs4959053 in PSORS1C1 gene was found between AITD patients and controls.Conclusion These data revealed the influence of PSORS1C1 on the susceptibility to AITDs and reinforced this locus as a common autoimmunity risk factor.