bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential Immunotherapeutic Targets for Hypoxia Due to COVI-Flu
Authors: Leyfman Yan , Erick Timothy K. , Reddy Sandeep S. , Galwankar Sagar , Nanayakkara Prabath W.B. , Di Somma Salvatore , Sharma Pushpa , Stawicki Stanislaw P. , Chaudry Irshad H. 
Published: July 2020

ABSTRACT
The world is currently embroiled in a pandemic of coronavirus disease 2019 (COVID-19), a respiratory illness caused by the novel betacoronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The severity of COVID-19 disease ranges from asymptomatic to fatal acute respiratory distress syndrome. In few patients, the disease undergoes phenotypic differentiation between 7 and 14 days of acute illness, either resulting in full recovery or symptom escalation. However, the mechanism of such variation is not clear, but the facts suggest that patient's immune status, comorbidities, and the systemic effects of the viral infection (potentially depending on the SARS-CoV-2 strain involved) play a key role. Subsequently, patients with the most severe symptoms tend to have poor outcomes, manifest severe hypoxia, and possess elevated levels of pro-inflammatory cytokines (including IL-1β, IL-6, IFN-γ, and TNF-α) along with elevated levels of the anti-inflammatory cytokine IL-10, marked lymphopenia, and elevated neutrophil-to-lymphocyte ratios. Based on the available evidence, we propose a mechanism wherein SARS-CoV-2 infection induces direct organ damage while also fueling an IL-6-mediated cytokine release syndrome (CRS) and hypoxia, resulting in escalating systemic inflammation, multi-organ damage, and end-organ failure. Elevated IL-6 and hypoxia together predisposes patients to pulmonary hypertension, and the presence of asymptomatic hypoxia in COVID-19 further compounds this problem. Due to the similar downstream mediators, we discuss the potential synergistic effects and systemic ramifications of SARS-CoV-2 and influenza virus during co-infection, a phenomenon we have termed “COVI-Flu.” Additionally, the differences between CRS and cytokine storm are highlighted. Finally, novel management approaches, clinical trials, and therapeutic strategies toward both SARS-CoV-2 and COVI-Flu infection are discussed, highlighting host response optimization and systemic inflammation reduction.
A Convolution Based Computational Approach Towards DNA N6-methyladenine Site Identification and Motif Extraction in Rice Genome
Authors: Rahman Chowdhury Rafeed , Amin Ruhul , Shatabda Swakkhar , Toaha Md. Sadrul Islam 
Published: July 2020

ABSTRACTDNA N6-methylation (6mA) in Adenine nucleotide is a post replication modification responsible for many biological functions. Automated and accurate computational methods can help to identify 6mA sites in long genomes saving significant time and money. Our study develops a convolutional neural network (CNN) based tool i6mA-CNN capable of identifying 6mA sites in the rice genome. Our model coordinates among multiple types of features such as PseAAC (Pseudo Amino Acid Composition) inspired customized feature vector, multiple one hot representations and dinucleotide physicochemical properties. It achieves auROC (area under Receiver Operating Characteristic curve) score of 0.98 with an overall accuracy of 93.97% using 5 fold cross validation on benchmark dataset. Finally, we evaluate our model on three other plant genome 6mA site identification test datasets. Results suggest that our proposed tool is able to generalize its ability of 6mA site identification on plant genomes irrespective of plant species. An algorithm for potential motif extraction and a feature importance analysis procedure are two by products of this research. Web tool for this research can be found at:https://cutt.ly/dgp3QTR.
Clustering fibromyalgia patients: A combination of psychosocial and somatic factors leads to resilient coping in a subgroup of fibromyalgia patients
Authors: Braun Alexandra , Evdokimov Dimitar , Frank Johanna , Pauli Paul , Üçeyler Nurcan , Sommer Claudia 
Published: July 2020

AbstractBackgroundCoping strategies and their efficacy vary greatly in patients suffering from fibromyalgia syndrome (FMS).ObjectiveWe aimed to identify somatic and psychosocial factors that might contribute to different coping strategies and resilience levels in FMS.Subjects and methodsStandardized questionnaires were used to assess coping, pain, and psychological variables in a cohort of 156 FMS patients. Quantitative real-time polymerase chain reaction (qRT-PCR) determined gene expression of selected cytokines in white blood cells of 136 FMS patients and 25 healthy controls. Data of skin innervation, functional and structural sensory profiles of peripheral nociceptive nerve fibers of a previous study were included into the statistics. An exploratory factor analysis was used to define variance explaining factors, which were then included into cluster analysis.Results54.9% of the variance was explained by four factors which we termed (1) affective load, (2) coping, (3) pain, and (4) pro-inflammatory cytokines (p < 0.05). Considering differences in the emerged factors, coping strategies, cytokine profiles, and disability levels, 118 FMS patients could be categorized into four clusters which we named “maladaptive”, “adaptive”, “vulnerable”, and “resilient” (p < 0.05). The adaptive cluster had low scores in disability and in all symptom categories in contrast to the vulnerable cluster, which was characterized by high scores in catastrophizing and disability (p < 0.05). The resilient vs. the maladaptive cluster was characterized by better coping and a less pro-inflammatory cytokine pattern (p < 0.05).ConclusionOur data suggest that problem- and emotion-focused coping strategies and an anti-inflammatory cytokine pattern are associated with reduced disability and might promote resilience. Additional personal factors such as low anxiety scores, ability of acceptance, and persistence further favor a resilient phenotype. Individualized therapy should take these factors into account.
A Holistic Approach to Identification of Covid-19 Patients from Chest X-Ray Images utilizing Transfer Based Learning
Authors: Rafi Taki Hasan 
Published: July 2020

AbstractNovel coronavirus likewise called COVID-19 began in Wuhan, China in December 2019 and has now outspread over the world. Around 8 millions of individuals previously got influenced by novel coronavirus and it causes at any rate 500,000 deaths. There are just about 90,000 individuals contaminated by COVID-19 in Bangladesh too. As it is an exceptionally new pandemic infection, its diagnosis is challenging for the medical community. In regular cases, it is hard for lower incoming countries to test cases easily. RT-PCR test is the most generally utilized analysis framework for COVID-19 patient detection. However, by utilizing X-ray image based programmed recognition can diminish the expense and testing time. So according to handling this test, it is important to program and effective recognition to forestall transmission to others. In this paper, we attempt to distinguish COVID-19 patients by chest X-ray images. We execute different pre-trained deep neural system models, for example, Sequential, DenseNet121, ResNet152 and EfficientNetB4 to assess the most productive outcome. And aims to utilize transfer-based learning. We assess this outcome by AUC, where EfficientNetB4 has 0.997 AUC, ResNet50 has 0.967 AUC, DenseNet121 has 0.874 AUC and the Sequential model has 0.762 AUC individually. And EfficientNetB4 has achieved 98.86% accuracy.
Restriction Point timing and cell cycle variability – a re-evaluation
Authors: Brooks Robert F. 
Published: July 2020

AbstractThe Restriction Point (R) in the mammalian cell cycle is regarded as a critical transition in G1 when cells become committed to enter S phase even in the absence of further growth factor stimulation. Classic time-lapse studies by Zetterberg and Larsson suggested that the acquisition of growth factor independence (i.e. passage of R) occurred very abruptly 3-4 hours after mitosis, with most cell cycle variability arising between R and entry into S phase. However, the cycle times of the post-R cells that continued on to mitosis after serum step-down without perturbation were far less variable than the control cells with which they were compared. A re-analysis of the data, presented here, shows that when the timing of R and entry in mitosis are compared for the same experiments, the curves are superimposable and statistically indistinguishable. This indicates that the data are compatible with the timing of R contributing to much of the overall variability in the cell cycle, contrary to the conclusions of Zetterberg and colleagues.
Gene expression profiles underlying aggressive behavior in the prefrontal cortex of cattle
Authors: Eusebi Paulina G. , Sevane Natalia , Pizarro Manuel , Valero Marta , Dunner Susana 
Published: July 2020

SummaryAggressive behavior is an ancient and conserved trait considered habitual and essential for most animals in order to eat, protect themselves from predators and also to compete for mating and defend their territories. Genetic factors have shown to play an important role in the development of aggression both in animals and humans, displaying moderate to high heritability estimates. However, although such types of conducts have been studied in different animal models, the molecular architecture of aggressiveness remains poorly understood. This study compared gene expression profiles of 16 prefrontal cortex (PFC) samples from aggressive and non-aggressive cattle breeds: Lidia, selected for agonistic responses, and Wagyu, specialized on meat production and selected for tameness. RNA sequencing was used to identify 918 up and 278 down-regulated differentiated expressed genes (DEG). The functional interpretation of the up-regulated genes in the aggressive cohort revealed enrichment of pathways such as the Alzheimer disease-presenilin, integrins or the ERK/MAPK signaling cascade, all implicated in the development of abnormal aggressive behaviors and neurophysiological disorders. Moreover, gonadotropins, leading to testosterone release, are also up-regulated as natural mechanisms enhancing aggression. Concomitantly, heterotrimeric G-protein pathways, associated with low reactivity mental states, and theGAD2gene, a repressor of agonistic reactions at PFC, are down-regulated, guaranteeing the development of the adequate responses required by the aggressive Lidia cattle. We also identified six upstream regulators, whose functional activity fits with the etiology of abnormal behavioral responses associated with aggression. These results provide valuable insights into the complex architecture that underlie naturally developed agonistic behaviors.
Solvent quality and chromosome folding inEscherichia coli
Authors: Xiang Yingjie , Surovtsev Ivan V. , Chang Yunjie , Govers Sander K. , Parry Bradley R. , Liu Jun , Jacobs-Wagner Christine 
Published: July 2020

SummaryAll cells must fold their genomes, including bacterial cells where the chromosome is compacted into a domain-organized meshwork called nucleoid. Polymer conformation depends highly on the quality of the solvent. Yet, the solvent quality for the DNA polymer inside cells remains unexplored. Here, we developed a method to assess this fundamental physicochemical property in live bacteria. By determining the DNA concentration and apparent average mesh size of the nucleoid, we provide evidence that the cytoplasm is a poor solvent for the chromosome inEscherichia coli. Monte Carlo simulations showed that such a poor solvent compacts the chromosome and promotes spontaneous formation of chromosomal domains connected by lower-density DNA regions. Cryo-electron tomography and fluorescence microscopy revealed that the (poly)ribosome density within the nucleoid is spatially heterogenous and correlates negatively with DNA density. These findings have broad implications to our understanding of chromosome folding and intracellular organization.
Positioning – a literature review
Authors: Saqib Natasha 
Published: July 2020

PurposeThe purpose of this paper is to review state-of-the-art literature on product/brand positioning to re-examine the positioning concept and developing a more comprehensive definition from a theoretical viewpoint.Design/methodology/approachA systematic review of positioning was conducted. The review conformed to a rigorous set of core principles: it was systematic (organized according to a method designed to address the review questions), transparent (explicitly stated), reproducible and updatable and synthesized (summarized the evidence relating to the review question).FindingsThe literature review reveals that there is lack of coherent definition for positioning, and there is no mutual agreement among marketing scholars and practitioners about the exact meaning of the concept. Therefore, comprehensive definition of positioning encompassing the five underlying positioning perspectives (competition; empty slot/mind; consumers’ perception, differentiation and competitive advantage) is suggested.Research limitations/implicationsThis paper will be useful for academicians to analyze the current nature of academic research in this area and will provide an added advantage to managers to design and implement positioning strategies for their product/brands that will allow their organizations to gain competitive advantage. This study acknowledges limitations with respect to its exclusive search criteria, which might affect its generalizability.Social implicationsPosition and positioning is of relevance in society in broad terms, e.g. in sports, politics and culture. Positioning strategy is discussed and implemented in different industries (business-to-business and consumer), for all kinds of brands (including, for instance, corporate brands) and for “brands” in the very widest sense (such as places or people).Originality/valueThis is the first systematic review of positioning that provides a detailed understanding of the current state of positioning research on a single platform and also draws a comprehensive positioning conceptualization.
The linkage between CSR and cost of equity: an Indian perspective
Authors: Dahiya Monika , Singh Shveta 
Published: July 2020

PurposeThis study aims to explore the relationship between corporate social responsibility (CSR) and the cost of equity (CoE) capital of Indian manufacturing firms.Design/methodology/approachThe study is conducted on a sample of 68 manufacturing firms listed on National Stock Exchange of India Limited (NSE) 200, investigated for the period 2013 to 2018. To deal with the issue of endogeneity, the techniques of system generalized method of moments and two-stage least square have been applied.FindingsThe results suggest that CSR disclosure is positively linked with the CoE in the case of manufacturing firms, signalling that socially responsible firms in India bear a higher CoE. The findings indicate that investors do not treat CSR as a value-augmenting factor.Practical implicationsFirms should effectuate effective managerial and organizational changes to fulfil their social responsibility instead of window dressing their activities. Regulators in India must work towards more stringent enforcement of the act and make efforts to promote public awareness of CSR.Social implicationsThe integration of CSR activities with the economic operations of the business is imperative.Originality/valueTo the best of researchers’ knowledge, there is a lack of studies focussing on India, which serves as an ideal setting for the study owing to the latest legislation mandating CSR expenditure. The study focusses on manufacturing firms as these firms are more susceptible to contribute to environmental pollution, exploitation of natural resources and labour concerns.
A scoping review of fall hazards in the homes of older adults and development of a framework for assessment and intervention
Authors: Keglovits Marian , Clemson Lindy , Hu Yi‐Ling , Nguyen An , Neff Anna J. , Mandelbaum Caren , Hudson Margaret , Williams Rebecca , Silianoff Tara , Stark Susan 
Published: July 2020

AbstractIntroductionComprehensive evaluation and intervention provided by occupational therapists is effective in reducing the presence of fall hazards in the homes of older adults. The purpose of this study was to document known environmental hazards and to update a previous content analysis. A secondary goal reviewed a framework for evaluation and practice.MethodsA comprehensive scoping review of published academic articles was performed from 1996 to 2019 to answer: What environmental hazards have been associated with falls in the homes of community‐dwelling older adults? Data was extracted in a standardised critical appraisal worksheet and content analysis was conducted. A review of a conceptual framework for assessment and intervention was conducted by international experts (n = 6) in face‐to‐face interviews.ResultsFourteen studies met the inclusion criteria for the scoping study. The studies reported 17 in‐home environmental hazards: throw rugs/carpets, clutter, cords/wires, poorly placed light switches, items placed too low, items placed too high, no grab bars, toilet seats too low, uneven floor surfaces, slippery/wet surfaces, snowy/icy surfaces, backless/unsupportive shoes, unsteady stairs, inadequate lighting, inadequate heating/cooling, step stools without railings, and pets.ConclusionA comprehensive list of specific fall hazards in and around the homes of older adults and a guiding framework offers occupational therapists an evidence‐based foundation for fall prevention efforts.
Prevalence of elevated liver enzymes and comorbidities in children and adolescents with overweight and obesity
Authors: Hartman Corina , Rennert Hedy S , Rennert Gad , Elenberg Yigal , Zuckerman Eli 
Published: July 2020

AbstractAimWe investigated the prevalence of elevated liver aminotransferases (ALT) and additional comorbidities in a large cohort of Israeli children and adolescents with overweight and obesity.MethodsThis study included data from medical records of 2‐ to 18‐year‐old children and adolescents, with body mass index (BMI) in the overweight or obesity range (WHO definitions), for whom ALT testing was performed.ResultsOverweight was present in 50 418 (10.7%) and obesity in 70 515 (15.0%). Elevated ALT, above 30 IU/L (0.51 μkat/L), was reported in 2245 (7.2%) of children with overweight and 5475 (16.8%) of children with obesity (P < .0001). Compared to children with overweight and obesity and ALT within normal range, children with elevated ALT were older (11.9 ± 4.2 vs 10.9 ± 4.2, P < .001), mostly male (68.0% vs 49.4%, P < .001) and had higher BMI (27.3 ± 6.1vs 24.0 ± 4.8, P < .001). They also had a more unfavourable cardiometabolic profile with significantly higher either systolic or diastolic blood pressure, total cholesterol and triglycerides, and had more than three criteria defining metabolic syndrome.ConclusionIn this large cohort, abnormally elevated ALT was present in a high number of individuals with overweight or obesity. The children and adolescents with abnormal ALT had higher BMI, were older, male and had more cardiometabolic risk factors.
Relationship quality and supply chain quality performance: The effect of supply chain integration in hotel industry
Authors: Le Shanghong , Wu Jinlin , Zhong Jianlan 
Published: July 2020

AbstractThis study investigates the relationship between relationship quality and supply chain quality performance in hotel supply chain, through the mediating effect of supply chain integration. A questionnaire survey is used to collect data relating to the research hypotheses. Structural equation model technique is suited for our research goals, and the SmartPLS software is implemented to test the conceptual model. The results show that relationship quality has a direct and positive impact on supply chain quality performance; but after introducing mediating variable—supply chain integration, relationship quality indirectly affects supply chain quality performance through supply chain integration. This means that a good relationship quality can promote supply chain integration and ultimately improve supply chain quality performance.
Beneath the surface: Application of transparent super absorbent polymer substrates to track faunal activity within the sediment layer
Authors: Mathers Kate L. , Michalec François‐Gaël , Holzner Markus , Weber Christine 
Published: July 2020

Abstract

Tracking the movement of organisms is a fundamental goal of many ecological studies. Several techniques exist in the study of terrestrial and aquatic fauna; however, to date, the ability to monitor aquatic fauna within the sediment layer efficiently and in multiple dimensions is lacking. Given the importance of subsurface sediments in supporting ecosystem functioning, this inability to observe organism behaviour represents a fundamental gap in our knowledge and limits our capability to holistically characterise the response of freshwater systems to stressors.

Here we present an experimental study that employs novel transparent super absorbent polymer substrates (c. 8–12 mm in diameter) in combination with computer vision technology, which enables, for the first time, real‐time observation and tracking of organisms within the sediment layer under lotic flow conditions. Use of these substrates allowed the successful extraction of organism trajectories, which enabled the velocity and body orientation of a freshwater amphipod (Gammarus fossarum) in the sediment layer to be calculated in response to a number of vertical hydrological exchange treatments (upwelling, downwelling, and no vertical exchange).

Results indicate that under vertical hydrological exchange, a higher proportion of fast velocities (both horizontal and vertical) were recorded for G. fossarum in the sediment layer compared to no vertical exchange (control) conditions. This increase was most marked for upwelling flow exchange. We also observed a change in the body orientation of individuals in the sediment layer from a vertical alignment under no vertical exchange to a more horizontal one under downwelling and more notably upwelling flow exchange. This shift in body position was exacerbated under stronger vertical exchange rates.

We identified that following the flow transition of downwelling to upwelling conditions, there was an immediate shift (0–2 min) in both the orientation angle and activity level of individuals. This increased rate of activity was maintained for the individuals' velocity but not for their changing orientation angle. These trends were not apparent within the flow transition of no vertical exchange to downwelling flow.

Our new methodological approach enables vital insights into the behaviour of organisms within the sediment layer. Use of super absorbent polymer substrates allows real‐time multi‐directional tracking of multiple organisms in parallel. We believe the method represents an innovative tool that can be employed to tackle a wide range of ecological questions and thereby improve our mechanistic understanding of ecological responses to biotic and abiotic processes/stressors.