bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heat stress on oocyte or zygote compromises embryo development, impairs interferon tau production and increases reactive oxygen species and oxidative stress in bovine embryos produced in vitro
Authors: Amaral Carolina S. , Koch Júlia , Correa Júnior Eduardo E. , Bertolin Kalyne , Mujica Lady K. S. , Fiorenza Mariani F. , Rosa Suzan G. , Nogueira Cristina W. , Comim Fábio V. , Portela Valério V. M. , Gonçalves Paulo B. D. , Antoniazzi Alfredo Q. 
Published: August 2020

AbstractInterferon tau (IFNT) is the cytokine responsible for the maternal recognition of pregnancy in ruminants and plays a role modulating embryo–maternal communication in the oviduct inducing a local response from immune cells. We aimed to investigate IFNT production, reactive oxygen species, and oxidative stress under the influence of heat stress (HS) during different stages of bovine in vitro embryo production. HS was established when the temperature was gradually raised from 38.5°C to 40.5°C in laboratory incubator, sustained for 6 hr, and decreased back to 38.5°C. To address the HS effects on IFNT production, reactive oxygen species, and oxidative stress, ovaries from a slaughterhouse were used according to treatments: control group (38.5°C); oocytes matured under HS; oocytes fertilized under HS; zygotes cultured in the first day under HS; and cells submitted to HS at oocyte maturation, fertilization, and the first day of zygote culture. The HS negatively affected cleavage and blastocyst rates, in all HS groups. On Day 7, all HS‐treated embryos showed decrease IFNT gene and protein expressions, whereas reactive oxygen species were increased in comparison to the control. In conclusion, the compromised early embryo development due to higher temperatures during in vitro oocyte maturation, fertilization, and/or zygote stage have diminished IFNT expression and increased reactive oxygen species in bovine.
Preparation of antimicrobial 3D printing filament: In situ thermal formation of silver nanoparticles during the material extrusion
Authors: Podstawczyk Daria , Skrzypczak Dawid , Połomska Xymena , Stargała Anna , Witek‐Krowiak Anna , Guiseppi‐Elie Anthony , Galewski Zbigniew 
Published: August 2020

AbstractMany efforts have been made either to provide the typical three‐dimensional (3D) printing materials with new and specific properties or to enhance their overall stability. The use of nanomaterials in 3D printing is gaining attention due to the tremendous functionality that this approach provides for those materials. This article presents a method of producing poly(lactic acid) (PLA)‐silver nanoparticle antibacterial filament for additive manufacturing. Polymer dope of silver salts within PLA was prepared by the solution‐phase and solvent‐less methods, followed by extrusion into filaments. Silver nanoparticles were synthesized in situ by thermal decomposition of silver salt during the thermal extrusion of the filament. The formation and dispersion of the nanoparticles within the polymer structure were examined and confirmed with transmission electron microscope and X‐ray diffraction. The solution‐phase method proposed in this study may prevent failure with nanoparticle dispersion occurring in melt blending. The filament was used for the fabrication of predesigned specimens for antimicrobial and mechanical testing. Printed objects exhibited antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. This study revealed that the incorporation of silver nanoparticles (0.01‐5 wt%) into PLA does not significantly change its bulk properties but renders the composite antibacterial. The filament may be used for manufacturing on‐demand customized antimicrobial objects.
In vivo and in silico characterization of apocynin in reducing organ oxidative stress: A pharmacokinetic and pharmacodynamic study
Authors: Liu Fangfei , Fan Lampson M. , Michael Nicholas , Li Jian‐Mei 
Published: August 2020

AbstractApocynin has been widely used in vivo as a Nox2‐contaninig nicotinamide adenine dinucleotide phosphate oxidase inhibitor. However, its time‐dependent tissue distribution and inhibition on organ reactive oxygen species (ROS) production remained unclear. In this study, we examined apocynin pharmacokinetics and pharmacodynamics (PKPD) after intravenous (iv) injection (bolus, 5 mg/kg) of mice (CD1, 12‐week). Apocynin was detected using a HPLC coupled to a linear ion‐trap tandem mass spectrometer. Apocynin peak concentrations were detected in plasma at 1 minute (5494 ± 400 ng/mL) (t1/2 = 0.05 hours, clearance = 7.76 L/h/kg), in urine at 15 minutes (14 942 ± 5977 ng/mL), in liver at 5 minutes (2853 ± 35 ng/g), in heart at 5 minutes (3161 ± 309 ng/g) and in brain at 1 minute (4603 ± 208 ng/g) after iv injection. These were accompanied with reduction of ROS production in the liver, heart and brain homogenates. Diapocynin was not detected in these samples. Therapeutic effect of apocynin was examined using a mouse model (C57BL/6J) of high‐fat diet (HFD, 16 weeks)‐induced obesity and accelerated aging. Apocynin (5 mmol/L) was supplied in drinking water during the HFD period and was detected at the end of treatment in the brain (5369 ± 1612 ng/g), liver (4818 ± 1340 ng/g) and heart (1795 ± 1487 ng/g) along with significant reductions of ROS production in these organs. In conclusion, apocynin PKPD is characterized by a short half‐life, rapid clearance, good distribution and inhibition of ROS production in major organs. Diapocynin is not a metabolite of apocynin in vivo. Apocynin crosses easily the blood‐brain barrier and reduces brain oxidative stress associated with metabolic disorders and aging.
Bidirectional connectivity via fish ladders in a large Neotropical river: Response to a comment
Authors: Fernandes Celestino Leandro , Sanz‐Ronda Francisco Javier , Miranda Leandro Esteban , Cavicchioli Makrakis Maristela , Dias João Henrique Pinheiro , Makrakis Sergio 
Published: August 2020

AbstractIn a recent article, we described fitting electronic tags to the fish Prochilodus lineatus to document how a fishway connected aquatic habitats downstream and upstream of a major dam. Moreover, given that tagged fish remained upstream or downstream for periods extending months and years before returning to the fishway, and that observed patterns of passage were consistent with seasonal migratory cycles, and building on existing literature, we speculated that the fishway allows fish access to spawning habitats upstream and feeding habitats downstream. Our interpretation of the movement data resulted in several comments from Pelicice, Pompeu, and Agostinho (2020) and they outline various reasons by which, in their opinion, some of our conclusions may be mistaken. Their critique is threefold. First, they argue that the percentage of fish attracted into the fishway is too low to consider the fishway an effective link between the reservoir and the river downstream. We contend that without estimates of population size it is impossible to judge if 28% passage is “limited”; conceivably, the absolute number of fish passed may still be enough to maintain a viable population. Second, they assert that because receivers were located only in the fishway it is unknown if fish that used the fishway remained near the dam, or if they continued their migration. We counter with a brief literature review that documents P. lineatus migrating through reservoirs and spawning in tributaries. Third, they advocate for a broader conservation perspective and for additional research. We agree and, in the article, had already expressed this view that fishways are only a temporary fix and that we support their use only as an element of a broader environmental management package. We also agree with the need for more research but argue that procrastinating on conservation action may not be wise because we do not know if the research will be done, how long it will take, or what the cost may be of waiting.
Association of history of cerebrovascular disease with severity of COVID-19
Authors: Siepmann Timo , Sedghi Annahita , Barlinn Jessica , de With Katja , Mirow Lutz , Wolz Martin , Gruenewald Thomas , Helbig Sina , Schroettner Percy , Winzer Simon , von Bonin Simone , Moustafa Haidar , Pallesen Lars-Peder , Rosengarten Bernhard , Schubert Joerg , Gueldner Andreas , Spieth Peter , Koch Thea , Bornstein Stefan , Reichmann Heinz , Puetz Volker , Barlinn Kristian 
Published: August 2020

Abstract
Objective
To determine whether a history of cerebrovascular disease (CVD) increases risk of severe coronavirus disease 2019 (COVID-19).

Methods
In a retrospective multicenter study, we retrieved individual data from in-patients treated March 1 to April 15, 2020 from COVID-19 registries of three hospitals in Saxony, Germany. We also performed a systematic review and meta-analysis following PRISMA recommendations using PubMed, EMBASE, Cochrane Library databases and bibliographies of identified papers (last search on April 11, 2020) and pooled data with those deriving from our multicenter study. Of 3762 records identified, 11 eligible observational studies of laboratory-confirmed COVID-19 patients were included in quantitative data synthesis.
Risk ratios (RR) of severe COVID-19 according to history of CVD were pooled using DerSimonian and Laird random effects model. Between-study heterogeneity was assessed using Cochran’s Q and I2-statistics. Severity of COVID-19 according to definitions applied in included studies was the main outcome. Sensitivity analyses were conducted for clusters of studies with equal definitions of severity.

Results
Pooled analysis included data from 1906 laboratory-confirmed COVID-19 patients (43.9% females, median age ranging from 39 to 76 years). Patients with previous CVD had higher risk of severe COVID-19 than those without [RR 2.07, 95% confidence interval (CI) 1.52–2.81; p < 0.0001]. This association was also observed in clusters of studies that defined severe manifestation of the disease by clinical parameters (RR 1.44, 95% CI 1.22–1.71; p < 0.0001), necessity of intensive care (RR 2.79, 95% CI 1.83–4.24; p < 0.0001) and in-hospital death (RR 2.18, 95% CI 1.75–2.7; p < 0.0001).

Conclusion
A history of CVD might constitute an important risk factor of unfavorable clinical course of COVID-19 suggesting a need of tailored infection prevention and clinical management strategies for this population at risk.
The revised version 2018 of the nationwide web-based registry system for kidney diseases in Japan: Japan Renal Biopsy Registry and Japan Kidney Disease Registry
Authors: Ozeki Takaya , Maruyama Shoichi , Nagata Michio , Shimizu Akira , Sugiyama Hitoshi , Sato Hiroshi , Yokoyama Hitoshi ,   the Committee for Renal Biopsy and Disease Registry of the Japanese Society of Nephrology
Published: August 2020

Abstract
Background
The Japan Renal Biopsy Registry (J-RBR), the first nation-wide registry of renal biopsies in Japan, was established in 2007, and expanded to include non-biopsy cases as the Japan Kidney Disease Registry (J-KDR) in 2009. The J-RBR/J-KDR is one of the biggest registries for kidney diseases. It has revealed the prevalence and distribution of kidney diseases in Japan. This registry system was meant to be revised after 10 years.

Methods
In 2017, the Committees of the Japanese Society of Nephrology started a project for the revision of the J-RBR/J-KDR. The revised system was designed in such a way that the diagnoses of the patients could be selected from the Diagnosis Panel, a list covering almost all known kidney diseases, and focusing on their pathogenesis rather than morphological classification. The Diagnosis Panel consists of 22 categories (18 glomerular, 1 tubulointerstitial, 1 congenital/genetical, 1 transplant related, and 1 other) and includes 123 diagnostic names. The items for clinical diagnosis and laboratory data were also renewed, with the addition of the information on immunosuppressive treatment.

Results
The revised version of J-RBR/J-KDR came into use in January 2018. The number of cases registered under the revised system was 2748 in the first year. The total number of cases has reached to 43,813 since 2007.

Conclusion
The revised version 2018 J-RBR/J-KDR system attempts to cover all kidney diseases by focusing on their pathogenesis. It will be a new platform for the standardized registration of kidney biopsy cases that provides more systemized data of higher quality.
Long-term follow-up of a randomised controlled trial of prolonged exposure therapy and supportive counselling for post-traumatic stress disorder in adolescents: a task-shifted intervention
Authors: Rossouw Jaco , Yadin Elna , Alexander Debra , Seedat Soraya 
Published: August 2020

AbstractBackgroundEmpirical evidence on the longer-term effectiveness of evidence-based treatments for adolescents with post-traumatic stress disorder (PTSD) in low-resource settings is needed. The aim of the study was to evaluate the maintenance of treatment gains achieved in a comparative study of effectiveness of prolonged exposure therapy for adolescents (PE-A) and supportive counselling (SC) in adolescents with PTSD up to 24-months post-treatment.MethodSixty-three adolescents (13–18 years) with PTSD were randomly assigned to receive either of the interventions comprising 7–14 sessions of treatment provided by trained and supervised non-specialist health workers (NSHWs). The primary outcome measure was PTSD symptom severity, as independently assessed on the Child PTSD Symptom Scale, at pretreatment, post-treatment, and at 3-, 6-, 12- and 24-months post-treatment follow-up (FU) evaluations.ResultsParticipants in both the prolonged exposure and SC treatment groups attained a significant reduction in PTSD symptoms and maintained this reduction in PTSD symptoms at 12- and 24-month assessment. Participants receiving prolonged exposure experienced greater improvement on the PTSD symptom severity scale than those receiving SC at 12-months FU [difference in PE-A v. SC mean scores = 9.24, 95% CI (3.66–14.83), p < 0.001; g = 0.88] and at 24-months FU [difference in PE-A v. SC mean scores = 9.35, 95% CI (3.53–15.17), p = 0.002; g = 0.68].ConclusionsAdolescents with PTSD continued to experience greater benefit from prolonged exposure treatment than SC provided by NSHWs in a community setting 12 and 24 months after completion of treatment.
The impact of demographic change in the balance between formal and informal old-age care in Spain. Results from a mixed microsimulation–agent-based model
Authors: Spijker Jeroen , Devolder Daniel , Zueras Pilar 
Published: August 2020

AbstractChanges in population and family structures are altering the provision of care for dependent older people. In Southern European countries like Spain, such care is still largely provided by family, typically spouses and adult daughters. However, an increasing proportion of women have entered the labour force, thereby affecting their availability. To study the demand and supply balance of informal care and to quantify the need for formal care when there is a deficit, we have developed a mixed microsimulation–agent-based model (ABM). Based on nuptiality, fertility and mortality levels of cohorts born at ten-year intervals between 1908 and 1968, the model starts with a microsimulation of the lifecycle of individuals and their close relatives until death. The ABM then determines the amount of time available or needed to care for family members, starting from age 50. Estimates are derived from Spanish survey data on employment, disability and time of care received. Surprisingly, results show that the family care deficit was greater in the older cohorts due to higher mortality and thus a greater impact of widowhood. However, for future elderly persons, we foresee that persistent below-replacement fertility and, paradoxically, the prolongation of the lifespan of couples will increase the demand for formal care as there will be more couples with both members incapacitated but without children to take care of them.
Development, validation and utilisation of dish-based dietary assessment tools: a scoping review
Authors: Shinozaki Nana , Yuan Xiaoyi , Murakami Kentaro , Sasaki Satoshi 
Published: August 2020

AbstractObjective:To summarise the existing evidence of development, validation and current status of utilisation of dish-based dietary assessment tools.Design:Scoping review.Setting:Systematic search using PubMed and Web of Science.Results:We identified twelve tools from seventy-four eligible publications. They were developed for Koreans (n4), Bangladeshis (n2), Iranians (n1), Indians/Malays/Chinese (n1), Japanese (n3) and Chinese Americans (n1). Most tools (10/12) were composed of a dish-based FFQ. Although the development process of a dish list varied among the tools, six studies classified mixed dishes based on the similarity of their characteristics such as food ingredients and cooking methods. Tools were validated against self-reported dietary information (n9) and concentration biomarkers (n1). In the eight studies assessing the differences between the tool and a reference, the mean (or median) intake of energy significantly differed in five studies, and 26–83 % of nutrients significantly differed in eight studies. Correlation coefficients for energy ranged from 0·15 to 0·87 across the thirteen studies, and the median correlation coefficients for nutrients ranged from 0·12 to 0·77. Dish-based dietary assessment tools were used in fifty-nine studies mainly to assess diet–disease relationships in target populations.Conclusions:Dish-based dietary assessment tools have exclusively been developed and used for Asian-origin populations. Further validation studies, particularly biomarker-based studies, are needed to assess the applicability of tools.
Cellular, mitochondrial and molecular alterations associate with early left ventricular diastolic dysfunction in a porcine model of diabetic metabolic derangement
Authors: Heinonen Ilkka , Sorop Oana , van Dalen Bas M. , Wüst Rob C. I. , van de Wouw Jens , de Beer Vincent J. , Octavia Yanti , van Duin Richard W. B. , Hoogstrate Youri , Blonden Lau , Alkio Milla , Anttila Katja , Stubbs Andrew , van der Velden Jolanda , Merkus Daphne , Duncker Dirk J. 
Published: August 2020

AbstractThe prevalence of diabetic metabolic derangement (DMetD) has increased dramatically over the last decades. Although there is increasing evidence that DMetD is associated with cardiac dysfunction, the early DMetD-induced myocardial alterations remain incompletely understood. Here, we studied early DMetD-related cardiac changes in a clinically relevant large animal model. DMetD was established in adult male Göttingen miniswine by streptozotocin injections and a high-fat, high-sugar diet, while control animals remained on normal pig chow. Five months later left ventricular (LV) function was assessed by echocardiography and hemodynamic measurements, followed by comprehensive biochemical, molecular and histological analyses. Robust DMetD developed, evidenced by hyperglycemia, hypercholesterolemia and hypertriglyceridemia. DMetD resulted in altered LV nitroso-redox balance, increased superoxide production—principally due to endothelial nitric oxide synthase (eNOS) uncoupling—reduced nitric oxide (NO) production, alterations in myocardial gene-expression—particularly genes related to glucose and fatty acid metabolism—and mitochondrial dysfunction. These abnormalities were accompanied by increased passive force of isolated cardiomyocytes, and impaired LV diastolic function, evidenced by reduced LV peak untwist velocity and increased E/e′. However, LV weight, volume, collagen content, and cardiomyocyte cross-sectional area were unchanged at this stage of DMetD. In conclusion, DMetD, in a clinically relevant large-animal model results in myocardial oxidative stress, eNOS uncoupling and reduced NO production, together with an altered metabolic gene expression profile and mitochondrial dysfunction. These molecular alterations are associated with stiffening of the cardiomyocytes and early diastolic dysfunction before any structural cardiac remodeling occurs. Therapies should be directed to ameliorate these early DMetD-induced myocardial changes to prevent the development of overt cardiac failure.
iPSC-derived progenitor stromal cells provide new insights into aberrant musculoskeletal development and resistance to cancer in down syndrome
Authors: Galat Yekaterina , Perepitchka Mariana , Elcheva Irina , Iannaccone Stephen , Iannaccone Philip M. , Galat Vasiliy 
Published: August 2020

AbstractDown syndrome (DS) is a congenital disorder caused by trisomy 21 (T21). It is associated with cognitive impairment, muscle hypotonia, heart defects, and other clinical anomalies. At the same time, individuals with Down syndrome have lower prevalence of solid tumor formation. To gain new insights into aberrant DS development during early stages of mesoderm formation and its possible connection to lower solid tumor prevalence, we developed the first model of two types of DS iPSC-derived stromal cells. Utilizing bioinformatic and functional analyses, we identified over 100 genes with coordinated expression among mesodermal and endothelial cell types. The most significantly down-regulated processes in DS mesodermal progenitors were associated with decreased stromal progenitor performance related to connective tissue organization as well as muscle development and functionality. The differentially expressed genes included cytoskeleton-related genes (actin and myosin), ECM genes (Collagens, Galectin-1, Fibronectin, Heparan Sulfate, LOX, FAK1), cell cycle genes (USP16, S1P complexes), and DNA damage repair genes. For DS endothelial cells, our analysis revealed most down-regulated genes associated with cellular response to external stimuli, cell migration, and immune response (inflammation-based). Together with functional assays, these results suggest an impairment in mesodermal development capacity during early stages, which likely translates into connective tissue impairment in DS patients. We further determined that, despite differences in functional processes and characteristics, a significant number of differentially regulated genes involved in tumorigenesis were expressed in a highly coordinated manner across endothelial and mesodermal cells. These findings strongly suggest that microRNAs (miR-24-4, miR-21), cytoskeleton remodeling, response to stimuli, and inflammation can impact resistance to tumorigenesis in DS patients. Furthermore, we also show that endothelial cell functionality is impaired, and when combined with angiogenic inhibition, it can provide another mechanism for decreased solid tumor development. We propose that the same processes, which specify the basis of connective tissue impairment observed in DS patients, potentially impart a resistance to cancer by hindering tumor progression and metastasis. We further establish that cancer-related genes on Chromosome 21 are up-regulated, while genome-wide cancer-related genes are down-regulated. These results suggest that trisomy 21 induces a modified regulation and compensation of many biochemical pathways across the genome. Such downstream interactions may contribute toward promoting tumor resistant mechanisms.