bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of miRNA-200b on the proliferation and apoptosis of cervical cancer cells by targeting RhoA
Authors: He Lijie , Wang Jing , Chang Dandan , Lv Dandan , Li Haina , Feng Heqiang 
Published: October 2020

Abstract

Objective
This article aims to investigate the effect of miRNA-200b on the proliferation and apoptosis of cervical cancer cells by targeting RhoA.

Methods
HeLa cells of cervical cancer were divided into five groups: blank control group, negative control group (miRNA-200b mimic NC), miRNA-200b mimic group, RhoA-negative control group, and RhoA overexpression group. Cells were collected 48 h after transfection. The expression levels of miRNA-200b were detected by RT-PCR. Target relationship between miRNA-200b and RhoA was verified by the dual-luciferase reporter assay. RhoA mRNA and protein expression were detected by western blot and RT-PCR methods. Flow cytometry was used to detect the apoptosis of cells in each group, and the CCK8 method was used to detect the proliferation of cells in each group. The mRNA and protein expression of Bax and cyclin D1 were detected by RT-PCR and western blot.

Results
The results of the dual luciferase reporter assay showed that RhoA was the target gene of microRNA 200b. Compared with the blank control group and the miRNA-200b mimic-NC group, the proportion of apoptotic cells increased significantly in the miRNA-200b mimic group, and the proliferation of cells was inhibited (P < 0.05). After overexpression of RhoA, the percentage of apoptotic cells decreased and the ability of cell proliferation increased significantly (P < 0.05).

Conclusion
miRNA-200b can inhibit the proliferation and promote the apoptosis of cervical cancer cells by targeting the RhoA gene.
Rheumatoid arthritis increases the risk of pleural empyema
Authors: Liao Kuang-Ming , Lin Cheng-Li , Shen Te-Chun 
Published: October 2020

Abstract

Background
Rheumatoid arthritis (RA) can lead to various pulmonary manifestations. Evidence shows the possible association between RA and pleural empyema.

Methods
We conducted a retrospective cohort study to investigate the risk of pleural empyema in patients with RA. The RA group (n = 29,061) included newly diagnosed adult patients between 2000 and 2012. The comparison group (n = 1,16,244) included individuals without RA at a 1:4 ratio of frequency matched by age, gender, and diagnosis year. The occurrence of pleural empyema was monitored until the end of 2013.

Results
Patients with RA had a higher risk of developing pleural empyema than those without RA (23.6 vs 1.82 per 10,000 person-years, adjusted hazard ratio = 11.0, 95% confidence interval [CI] = 8.90–13.5). Furthermore, intensive care unit admission rates of pleural empyema were 37.7% in the RA group and 37.2% in the comparison group (adjusted odds ratio [OR] = 1.02, 95% CI = 0.66−1.57). The 30-day mortality rates of pleural empyema were 11.2% in the RA group and 10.9% in the comparison group (adjusted OR = 1.01, 95% CI = 0.51−1.88).

Conclusion
Patients with RA are at a greater risk of developing pleural empyema than those without RA.
The Selfish Meme: Dawkins, Peirce, Freud
Authors: West Joel 
Published: October 2020

Abstract
Biologist Richard Dawkins coined the term “meme” by which he meant a unit of culture. Dan Dennett continued by defining a meme as a bunch of bits of information. This paper explores the “meme” and how it is semiotic, both in its technical sense and in its popular sense and explores how memes signify both in terms of classical semiotics and also in terms of post-structuralist thought.
Bromide hydrogen oxalate salts with the diprotonated 1,4-diazabicyclo[2.2.2]octane counterion
Authors: Ndiaye Mamadou , Diop Mouhamadou Birame , Samb Abdoulaye , Diop Libasse , Oliver Allen G. , Plasseraud Laurent 
Published: October 2020

Abstract
Two new salts composed of the diprotonated 1,4-diazabicyclo[2.2.2]octane (DABCO) molecule as the cations and bromide and hydrogen oxalate as the anions have been isolated and structurally characterized by X-ray diffraction analysis. The salt [DABCOH2]{[HC2O4][Br]} (1) crystallizes in the orthorhombic system, space group P212121 with a = 9.0809(7), b = 9.5156(7), c = 12.3558(9) Å, V = 1067.67(14) Å3 and Z = 4. The salt [DABCOH2]2{[HC2O4][Br]3}·H2O (2) crystallizes in the orthorhombic system, space group Pnma with a = 26.6554(17), b = 7.3711(4), c = 10.7421(7) Å, V = 2110.6(2) Å3 and Z = 4. The compounds were prepared from ethanolic solutions of [DABCOH2][HC2O4]2 (L

1
) and ZnBr2 in molar ratios of 2:1 and 1:1, respectively. The salts 1 and 2 exhibit extended hydrogen bonding networks leading to supramolecular topologies.
Targeted Stimulation of an Orbitofrontal Network Disrupts Decisions Based on Inferred, Not Experienced Outcomes
Authors: Wang Fang , Howard James D. , Voss Joel L. , Schoenbaum Geoffrey , Kahnt Thorsten 
Published: October 2020

When direct experience is unavailable, animals and humans can imagine or infer the future to guide decisions. Behavior based on direct experience versus inference may recruit partially distinct brain circuits. In rodents, the orbitofrontal cortex (OFC) contains neural signatures of inferred outcomes, and OFC is necessary for behavior that requires inference but not for responding driven by direct experience. In humans, OFC activity is also correlated with inferred outcomes, but it is unclear whether OFC activity is required for inference-based behavior. To test this, we used noninvasive network-based continuous theta burst stimulation (cTBS) in human subjects (male and female) to target lateral OFC networks in the context of a sensory preconditioning task that was designed to isolate inference-based behavior from responding that can be based on direct experience alone. We show that, relative to sham, cTBS targeting this network impairs reward-related behavior in conditions in which outcome expectations have to be mentally inferred. In contrast, OFC-targeted stimulation does not impair behavior that can be based on previously experienced stimulus–outcome associations. These findings suggest that activity in the targeted OFC network supports decision-making when outcomes have to be mentally simulated, providing converging cross-species evidence for a critical role of OFC in model-based but not model-free control of behavior.SIGNIFICANCE STATEMENTIt is widely accepted that the orbitofrontal cortex (OFC) is important for decision-making. However, it is less clear how exactly this region contributes to behavior. Here we test the hypothesis that the human OFC is only required for decision-making when future outcomes have to be mentally simulated, but not when direct experience with stimulus–outcome associations is available. We show that targeting OFC network activity in humans using network-based continuous theta burst stimulation selectively impairs behavior that requires inference but does not affect responding that can be based solely on direct experience. These results are in line with previous findings in animals and suggest a critical role for human OFC in model-based but not model-free behavior.
Eosinophil otitis media – egy kevéssé ismert kórkép kezelési nehézségei
Authors: Révész Péter , Gerlinger Imre , Kálmán Endre , Koukkoullis Alexandros , Burián András , Tóth István 
Published: October 2020

Absztrakt:
Az eosinophil otitis media az utóbbi évtizedben került a kutatók és a klinikum
látóterébe; jellemzője a középfülben termelődő, eosinophil granulocytákkal
telített mucosus, nehezen eltávolítható váladék és az asthma bronchiale,
valamint a krónikus rhinosinusitis társulása. A betegség egyelőre kevéssé ismert
a klinikumban, emiatt nincsenek információk a pontos előfordulási gyakoriságáról
sem. A kórkép nem gyógyítható, de kezelésére beváltak az intratympanalisan
használható szteroidkészítmények, melyek azonban csak enyhébb fokú betegség
esetén vezetnek eredményre. A súlyosabb gradusú eosinophil otitis media dobüregi
granulációs szövet képződésével és idegi halláscsökkenéssel is járhat, ami
sebészi eltávolítást és per os szteroidkezelést is igényel.
Fontos felhívni a figyelmet a kórkép létezésére, mert a megfelelő diagnózis
felállítása után az időben elkezdett kezeléssel elkerülhető lehet az
életminőséget rontó fülváladékozás, felülfertőződés és az idegi halláscsökkenés.
A jelen közleményben a szerzők a nemzetközi szakirodalom áttekintésén túl saját
esetbemutatásokon keresztül demonstrálják a betegség enyhe és súlyos fokú
változatának kezelési lépéseit. Orv Hetil. 2020; 161(41): 1769–1775.
Stigma and Abortion in Argentina
Authors: Drovetta Raquel Irene 
Published: October 2020

This paper examines the stigma of abortion among professionals who are providers of the service, considering, in particular, the time when they joined the Network of Healthcare Professionals for the Right to Decide. This network was created in Argentina at the end of 2014 by a joint alliance of women’s and feminist organizations and healthcare professionals. We have conducted an exploratory study, of a descriptive type, based on a qualitative strategy. From 2016 to 2018, we carried out interviews with healthcare professional, with a view to addressing the relationship between the stigma of abortion and the healthcare professionals who are members of the Network. The main results indicate that the professionals have changed their perception of the stigma of abortion by becoming members of a group made up of peers among whom there exists homogeneity regarding their goals, and objectives in their profession. They admit to gaining confidence to resist stigma indicators, such as a negative self-perception of the job done, the fear of information disclosure and the silencing of, and detachment from fellow professionals. The experience permits to show how it is possible to resist stigmatization effects, by performing an active role regarding professional identity.
EARLY PREDICTORS OF HOSPITAL MORTALITY IN PATIENTS WITH COVID-19 PNEUMONIA AT A LEVEL III HOSPITAL. LIMA, PERU.
Authors: Ravelo-Hernández Jorge , Cáceres-Bernaola Ursula , Becerra-Nuñez Claudia , Mendívil-Tuchia Sabina , Vásquez-Rivas María Eugenia , Aguirre-Quispe Wilfor , Quispe-Ayuque Edwin , Reyes-Rocha Gabriela 
Published: October 2020

Objective: Determine whether the tomographic characteristics of patients with COVID-19 pneumonia at hospital admission and the initial tomographic severity score (TSS) as well as some laboratory tests or clinical characteristics predict mortality and length of hospital stay.Material and methods: Analytical, retrospective study that included 203 patients with a clinical diagnosis of SARSCoV2 Virus Infection, performed by reverse transcriptase polymerase chain reaction (RT-PCR), reactive serological test (IgM/IgG) and/or spiral computed tomography (CT) chest without contrast, admitted in the period from 6 April to 27 June 2020. Two radiologists (blind evaluators) described the tomographic findings. 15 patients with normal CT were excluded, 188 patients with clinical and laboratory parameters were admitted to the statistical analysis of TSS and mortality; data taken from epidemiological records and clinical histories, divided into two groups: recovered and deceased. Data analysis used the statistical package Stata version 14.2; hospital stay was analyzed by Kaplan – Meier curves and mortality prediction was made by Cox regression with 95% CI and a p value <0.05 as statistically significant.Results: Hypertension was the most frequent associated disease, the most common clinical presentation included cough, malaise, fever and dyspnea , there were no significant differences between studied groups (Recovered vs. death patients), 15 cases had normal CT, for this reason, they were excluded from the TSS score and mortality analysis. Mean age of the recovered patients was 53.6 ± 16.4 years and of the deceased 75.9 ± 13.9 years (p <0.0001). A moderate and high TSS (≥ 8) resulted in deaths (p <0.05), as well as a higher degree of lymphopenia and a history of asthma in the deceased (p <0.05). The variable arterial hypertension predicts the increase in days of hospital stay (p <0.05). The ground glass pattern was the most frequent, followed by the consolidation and distortion of the architecture; however, they were not associated with a type of outcome. The pattern of pleural effusion and bronchial dilation showed a significant difference with respect to the outcome, however, but it could be due to the infrequency of presentation of these patterns. Conclusions: TSS is useful in the initial and comprehensive diagnostic evaluation of COVID-19 pneumonia, in conjunction with markers such as lymphopenia and elevated CRP that can predict a poor short-term outcome. A high TSS score is a predictor of mortality. Hypertension predicts the increase in days of hospital stay.
TRANSIENT ANALYSIS OF SIMULTANEOUS LOFA AND RIA IN RSG-GAS REACTOR AFTER 32 YEARS OPERATION
Authors: Isnaini Muhammad Darwis , Kuntoro Iman , Subekti Muhammad 
Published: October 2020

During the operation of the research reactor RSG-GAS, there are many design parameters should be verified based on postulated accidents. Several design basis accidents (DBA) such as loss of flow accident (LOFA) and reactivity-initiated accident (RIA) also have been conducted separately. This paper discusses about possibility of simultaneous accidents of LOFA and RIA. The accident analyses carry out calculation for transient condition during RIA, LOFA, and postulated accident of simultaneous LOFA-RIA. This study aims to conduct a safety analysis on simultaneous LOFA and RIA, and investigate the impact on safety margins. The calculations are conducted by using the PARET code. The maximum temperature of the center fuel meat at nominal power of 30 MW and steady state conditions is 126.10°C and MDNBR of 2.94. At transients condition, the maximum center fuel meat temperature for LOFA, RIA and simultaneous LOFA-RIA are consecutively 132.99°C, 135.67°C and 138.21°C, and the time of reactor trip are 3.2593s, 3.6494s and 2.7118s, respectively. While the MDNBR for LOFA, RIA and simultaneous LOFA-RIA are respectively at transient condition are 2.88, 2.58 and 2.63, respectively. It is shown that, simultaneous LOFA-RIA has the fastest trip time. In this case, the low flow trip occurs first in advance to over power trip. From these results, it can be concluded that the RSG-GAS has adequate safety margin against transient of simultaneous LOFA-RIA.Keywords: RSG-GAS, Simultaneous, LOFA, RIA, PARET
Magnitude Calculation without Saturation from Strong-Motion Waveforms
Authors: Hongcai Zhang , Melgar Diego , Goldberg Dara E. 
Published: October 2020

ABSTRACTAfter destructive earthquakes, it is a challenge to estimate magnitude rapidly and accurately for dissemination to emergency responders and the public. Here, we propose criteria to calculate peak ground displacement (PGD) from strong-motion records, which can be used to calculate unsaturated event magnitude. Using collocated strong-motion and Global Navigation Satellite Systems observations of five major earthquakes in Japan, we demonstrate the effectiveness and accuracy of our strategy. Our results show that, with the right filtering criteria, PGD estimated from strong-motion acceleration waveforms is consistent with geodetic estimates. The methodology, however, does not allow for calculation of reliable estimates of coseismic deformation or other ground displacement metrics. We demonstrate a simulated real-time magnitude estimation that suggests it is feasible to generate an unsaturated magnitude estimate in real time from near-field strong-motion records. These findings have important implications for early warning and emergency response in seismically active areas, especially where real-time strong-motion data are more widely available than geodetic measurements.
MEDIDAS MODERNIZADORAS NO RECIFE DO SÉCULO XIX: ordenamento, progresso e civilidade
Authors: DUARTE JOSÉ LINS 
Published: October 2020

O presente artigo objetiva analisar os desdobramentos do Recife no século XIX, mediante a necessidade de implementar melhorias urbanas que envolvessem os aparatos das tecnologias em curso e as medidas de higienização, a fim de atender às carências de uma urbe que se encontrava tencionada pelo adensamento populacional. Além disso, visa avaliar determinadas observações emitidas por estrangeiros e citadinos, quanto às impressões do espaço urbano e à interação com a população. Igualmente, considerar aspectos que levaram, em certo momento, haver maior aproximação entre parcela da sociedade e governo, motivados pela ideia de progresso.
Palavras-chave: Urbe. Espaço Urbano. Progresso. Higienização.
O COTIDIANO DO HOSPITAL DE CARIDADE DE SÃO LUÍS E A CRISE DA ASSISTÊNCIA NA SEGUNDA METADE DO SÉCULO XIX
Authors: COE AGOSTINHO JÚNIOR HOLANDA 
Published: October 2020

A Irmandade da Misericórdia foi fundada em São Luís por volta de 1623. Em 1642, a Irmandade já havia construído uma igreja e um cemitério e, em 1653, já haviam projetos para a construção de um hospital. No século XIX administrava um cemitério, o Hospital de Caridade e dos Lázaros, além da Roda e Casa dos Expostos. Diante de tal crescimento do poderio e importância da assistência praticada pelos congregados na Irmandade da Misericórdia, analisaremos como o tratamento dispensado aos doentes no Hospital de Caridade foi se tornando cada vez mais um “grande fardo” para a Misericórdia, já que, na segunda metade do século XIX, as reclamações eram recorrentes acerca da dificuldade do cuidado para com os enfermos em virtude do acirramento de crises financeiras. Como o século XIX, em São Luís, foi marcado pelas diversas epidemias que assolavam os aglomerados urbanos, também estudaremos a assistência no contexto dos surtos epidêmicos e as transformações que tais epidemias proporcionaram ao Hospital de Caridade.
Palavras-chave: Hospital. Caridade. Assistência. Crise.
PERUBAHAN PERILAKU GROOMING DAN IMOBILITAS MENCIT BALB/C TERINDUKSI DEPRESI YANG DISUPLEMENTASI TEMPE SEBAGAI SUMBER PARAPROBIOTIK
Authors: Ardianty Vanessa , Manurung Brian Saputra 
Published: October 2020

Depression, a mental disorder marked by sadness, anhedonia and increased fatigability, is becoming more common in this industry 4.0 era. Nowadays, depression affects approximately 322 million people around the world (more than 14 million in Indonesia) and gives major contribution to the rise of global burden of disease. Although antidepressant is considered the common treatment for depression, recent studies show a possibility to treat depression by altering gut microbiome of the patient, by giving them probiotic food. Tempeh is a globally well-known Indonesian paraprobiotic food which already proven to modulate gut microbiota. This research aimed to find out the effect of tempeh to depression symptom as expressed in Balb/c mice behaviors. The methods used was to feed tempeh or tempeh starter to mice which were depressed-induced by Unpredictable Chronic Mild Stress (UCMS) procedure. Parameter measured were depression level of the Balb/c mice based on immobility times and grooming durations acquired from tail suspension test, forced swim test, sucrose splash test and coat state score. The result showed that tempeh supplementation did not affect coat states and grooming durations but tend to improve immobility times of the Balb/c mice. Meanwhile, tempeh starter supplementation tends to improve the immobility time and kept coat state clean/tidy, but lowered grooming duration. In conclusion, supplementation of paraprobiotic tempeh, especially in starter form, tend to improve depression symptoms.
Keywords: depression, mice, paraprobiotic, tempeh
The Vent-Lock Protocol: 3D Printing and Testing of A Ventilator Multiplexer to Enhance the Capacity of Treating Patients with COVID-19&nbsp;
Authors: Xun Helen , Shallal Christopher , Unger Justin , Tao Runhan , Torres Alberto , Vladimirov Michael , Frye Jenna , Singhala Mohit , Horne Brockett , Yesantharao Pooja , Kim Bo Soo , Burke Broc , Montana Michael , Talcott Michael , Winters Bradford , Frisella Margaret , Kushner Bradley , Sacks Justin , Guest James , Kang Sung Hoon , Caffrey Julie 
Published: October 2020

Abstract
Mechanical ventilators are essential to patients who become critically ill from acute respiratory distress syndrome (ARDS), and shortages have been reported due to the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We utilized cost-effective, on-demand 3D printing (3DP) technology to produce critical components for a novel ventilator multiplexer system, Vent-Lock, to split one ventilator or anesthesia gas machine between two patients. FloRest, a novel 3DP flow restrictor, provides clinicians control of tidal volumes and positive end expiratory pressure (PEEP), using the 3DP manometer adaptor to monitor pressures. We tested the ventilator splitter circuit in simulation centers between artificial lungs and used an anesthesia gas machine to successfully ventilate two swines. As one of the first studies to demonstrate splitting one anesthesia gas machine between two swines, we present proof-of-concept of a de novo, closed, multiplexing system, with flow restriction for individualized patient therapy. Our studies underscore that while possible, ventilator multiplexing is a complicated synergy between machine settings, circuit modification, and patient monitoring. Consequently, ventilator multiplexing is reserved only as a last emergency resource, by trained clinicians and respiratory therapists with ventilator operative experience.
Information Processing of Health Warning Messages on Cigarette Packs Among Young Iranian Smokers: An Application of Elaboration Likelihood Model
Authors: Shahi Fatemeh , Pourrazavi Sara , Kouzekanani Kamiar , Jafarabadi Mohammad Asghari , Allahverdipour Hamid 
Published: October 2020

Abstract
Background: One of the major smoking prevention strategies has been to educate the public and increase people’s awareness, using health warning messages. However, many young people continue smoking without paying attention to health risk messages on cigarette packets in Iran. Hence, this study was conducted to examine the processing route of anti-smoking messages and influencing cognitive factors by using the Elaboration Likelihood Model.Methods: The non-probability sample for the cross-sectional study consisted of 387 smokers in the age range of 18 to 30 years old in Tabriz, Iran. A researcher-designed questionnaire was used for the purpose of data collection. No causal inferences were drawn due to the non-experimental nature of the study.Results: It was found that smokers often processed warning messages through the central route. Perceived severity, smoking abstinence self-efficacy, and psychological dependence were predictors of message processing through the central route. The results supported the conceptual model of cognitional predictors of the processing route.Conclusions: To design and execute effective health warning messages to quit smoking, it is recommended to consider cognitive factors as a means to enhance critical thinking about the content of the message.