bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examination of the Role of Cells in Commercially Available Cellular Allografts in Spine Fusion
Authors: Abedi Aidin , Formanek Blake , Russell Nicholas , Vizesi Frank , Boden Scott D. , Wang Jeffrey C. , Buser Zorica 
Published: October 2020

Background:
Despite the extensive use of cellular bone matrices (CBMs) in spine surgery, there is little evidence to support the contribution of cells within CBMs to bone formation. The objective of this study was to determine the contribution of cells to spinal fusion by direct comparisons among viable CBMs, devitalized CBMs, and cell-free demineralized bone matrix (DBM).

Methods:
Three commercially available grafts were tested: a CBM containing particulate DBM (CBM-particulate), a CBM containing DBM fibers (CBM-fiber), and a cell-free product with DBM fibers only (DBM-fiber). CBMs were used in viable states (CBM-particulatev and CBM-fiberv) and devitalized (lyophilized) states (CBM-particulated and CBM-fiberd), resulting in 5 groups. Viable cell counts and bone morphogenetic protein-2 (BMP-2) content on enzyme-linked immunosorbent assay (ELISA) within each graft material were measured. A single-level posterolateral lumbar fusion was performed on 45 athymic rats with 3 lots of each product implanted into 9 animals per group. After 6 weeks, fusion was assessed using manual palpation, micro-computed tomography (μ-CT), and histological analysis.

Results:
The 2 groups with viable cells were comparable with respect to cell counts, and pairwise comparisons showed no significant differences in BMP-2 content across the 5 groups. Manual palpation demonstrated fusion rates of 9 of 9 in the DBM-fiber specimens, 9 of 9 in the CBM-fiberd specimens, 8 of 9 in the CBM-fiberv specimens, and 0 of 9 in both CBM-particulate groups. The μ-CT maturity grade was significantly higher in the DBM-fiber group (2.78 ± 0.55) compared with the other groups (p < 0.0001), while none of the CBM-particulate samples demonstrated intertransverse fusion in qualitative assessments. The viable and devitalized samples in each CBM group were comparable with regard to fusion rates, bone volume fraction, μ-CT maturity grade, and histological features.

Conclusions:
The cellular component of 2 commercially available CBMs yielded no additional benefits in terms of spinal fusion. Meanwhile, the groups with a fiber-based DBM demonstrated significantly higher fusion outcomes compared with the CBM groups with particulate DBM, indicating that the DBM component is probably the key determinant of fusion.

Clinical Relevance:
Data from the current study demonstrate that cells yielded no additional benefit in spinal fusion and emphasize the need for well-designed clinical studies on cellular graft materials.
Performance of Mattis dementia rating scale-Chinese Version in patients with mild cognitive impairment and Alzheimer's disease&nbsp;
Authors: QIAN shuxia , CHEN keliang , GUAN qiaobing , Guo qihao 
Published: October 2020

Abstract
Background: To identify the applicability of the Chinese Version of Mattis Dementia Rating Scale (DRS-CV).Methods: The DRS-CV was administered to 483 participants, including 136 normal controls, 167 patients with mild cognition impairment (MCI), and 180 patients with Alzheimer’s disease (AD). Receiver Operating Characteristic (ROC) curve was used to evaluate the sensitivity and specificity of the scale.Results: The scores of DRS-CV were ranked in the order of NC >MCI > mild AD > moderate AD group. Memory was the sensitive function affected at a relatively earlier stage of AD. ROC curve analysis indicated the DRS-CV total score and memory subscale showed excellent sensitivity and specificity in the discrimination between MCI from mild AD and mild AD from moderate AD, but poor sensitivity and specificity in the discrimination between MCI and NC.Conclusion: The DRS-CV is useful to the early diagnosis and severity of AD, not to the early identification of MCI.
A single Epley manoeuvre can improve self-perceptions of disability (quality of life) in patients with cp-BPPV: A randomised controlled trial in primary care.
Authors: Muñoz Ricard Carrillo , Moreno Jose Luis Ballve , Balboa Ivan Villar , Matos Yolanda Rando , Puertolas Oriol Cunillera , Perez Estrella Rodero , Curto Xavier Monteverde , Ripollès Carles Rubio , Farres Noemi Moreno , Agudelo Olga Lucia Arias , Ortega Jesus Almeda 
Published: October 2020

Abstract
Background: Posterior canal benign paroxysmal positional vertigo (pc-BPPV) causes physical, functional, and emotional impairment. The treatment of choice is the Epley manoeuvre (EM). The purpose of the study was to compare the impact of the EM and a sham manoeuvre in primary care on self-perceived disability. Method: Randomised, double-blind, sham-controlled clinical trial conducted in primary care with a follow-up of 1 year. Patients aged ≥18 years old diagnosed with pc-BPPV according to the Dix-Hallpike test (DHT) were randomised to an intervention (EM) group or a control (sham manoeuvre) group. The main study covariables were age, sex, history of depression and anxiety, presence of nystagmus in the DHT, patient-perceived disability assessed with the Dizziness Handicap Inventory-screening version (DHI-S). Data were analyzed using bivariate and multivariate mixed Tobit analyses. Results: Overall, 134 patients were studied: 66 in the intervention group and 68 in the control group. Median age was 52 years (interquartile range [IQR], 38.25–68.00 years) and 76.12% of the patients were women. The DHT triggered nystagmus in 40.30% of patients. The median total DHI-S score for the overall sample at baseline was 16 (IQR, 8.00–22.00); 16 [IQR, 10.5–24.0] vs 10 [6.0–14.0] for women vs men ( P <0.001) and 16 [IQR, 10.0-24.0] vs 12 [IQR, 8.0–18.0] for patients without nystagmus vs those with nystagmus ( P =0.033).Patients treated with the EM experienced a mean reduction of 2.03 points in DHI-S score over the follow-up period compared with patients in the sham group. Conclusion: Pc-BPPV affects the quality of life of primary care patients. A single EM can improve self-perceptions of disability by around 2 points on the DHI-S scale, Trial registration : ClinicalTrials.gov Identifier: NCT01969513. Retrospectively registered. First Posted: October 25, 2013. https://clinicaltrials.gov/ct2/show/NCT01969513
The relationship between time to surgery after neoadjuvant chemotherapy and survival in breast cancer patients:a meta-analysis
Authors: Su YongCheng , Zheng XiaoGang 
Published: October 2020

Abstract
PURPOSE: This meta-analysis aims to evaluate the impact of delaying surgery in operable breast tumor patients after neoadjuvant chemotherapy (NAC) on survival. METHODS:An electronic literature retrieval was conducted on PubMed/Medline and EMBASE((between January 2000 and June 2020).The primary end point was overall survival(OS),secondary end points included disease-free survival (DFS) or recurence-free survival (RFS).The HR with 95% confidence intervals were calculated using a random-effects or fixed-effects model. RESULTS:The combined HR for OS was 1.51 (95% CI:1.30-1.76; P=0.000) by fixed effects model.No statistically significant heterogeneity was found (P=0.168, I 2 =31.3%).The pooled HR for RFS/DFS was 1.59 (95%CI:1.30-1.95,I 2 = 66.0%) by random-effects model,with significant heterogeneity. CONCLUSION:Our meta-analysis revealed a significant adverse association between longer TTS after NAC and more inferior OS and RFS/DFS in patients with breast cancer.Clinicians and patients should minimize surgical delay after NAC as much as possible.
Student Lecture Attendance and Its Relation to Exam Performance in the Era of Lecture Capture Technology.
Authors: Powers Claud A , Carroll Mairead A. 
Published: October 2020

Abstract
Background: Students can now review on-line lecture recordings rather than attend lectures. However, there is little data on the fraction of students attending lectures, the patterns of attendance, or the ultimate learning outcomes. To study this issue, attendance data was used to compare learning outcomes of students with different patterns of attendance.Methods: A retrospective observational analysis was performed using data collected in the Medical Pharmacology course in Fall 2013 (197 students) and Fall 2014 (207 students). Attendance was monitored at 13 of 40 lectures with an audience response system in each semester. All lectures were recorded using lecture-capture, and students could attend lectures or review online recordings and PowerPoint files as desired. Exam averages of students in different attendance categories were compared, along with the frequency distribution of exam scores in the different attendance categories. Results: In the years studied, 12-14% of the students had a consistent pattern of high attendance at monitored lectures; 35-46% of the students had an erratic pattern of low to intermediate attendance, and 41-52% of the students did not attend any monitored lectures. Aggregate exam averages of students with >80% attendance were over 5 percentage points higher than those of students in other attendance categories (P<0.05 using t-tests or ANOVA). Students with >80% attendance also displayed a significant shift in aggregate exam averages to higher ranks in frequency distribution analyses (P<0.05 using Chi-Square tests). Exam averages of students with low to intermediate attendance did not differ from students with no attendance. The scoring advantage associated with >80% lecture attendance remained evident 20 weeks after the Fall semester when students took a comprehensive pharmacology examination (P<0.05). Conclusions: On average, students with a consistent pattern of high lecture attendance had higher exam scores than students with low to intermediate or no attendance who had access to the identical course material on-line. This may reflect factors related to the method of content acquisition (live vs. on-line) or differences in the learning readiness of students choosing one approach vs. another. The data suggest that curricular changes that encourage consistent lecture attendance might improve learning outcomes for some students.
Risk Factors for Mortality in Critically Ill Patients with COVID-19: A Multicenter Retrospective Case-Control Study
Authors: Gao Jinghua , Zhong Li , Wu Ming , Ji Jingjing , Shao Ziyun , Liu Zheying , Wang Conglin , Xie Qifeng , Liu Zhifeng 
Published: October 2020

Abstract
Background

Coronavirus disease 2019 (COVID-19) has spread around the world and caused many deaths, but little is known about the risk factors for death in critically patients.
Methods

we collected demographic and clinical data on all severe inpatients with COVID-19 .We used univariable and multivariable Cox regression methods to determine the independent risk factors related to likelihood of 28-day and 60-day survival, performing survival curve analysis.
Results

Of 325 patients enrolled in the study, Multi-factor Cox analysis showed increasing odds of in-hospital death associated with basic illness (hazard ratio [HR] 6.455, 95% Confidence Interval [CI] 1.658–25.139, P = 0.007), lymphopenia (HR 0.373, 95% CI 0.148–0.944, P = 0.037), higher Sequential Organ Failure Assessment (SOFA) score on admission (HR 1.171, 95% CI 1.013–1.354, P = 0.033) and being critically ill (HR 0.191, 95% CI 0.053–0.687, P = 0.011). Increasing 28-day and 60-day mortality, declining survival time and more serious inflammation and organ failure were associated with lymphocyte count ≤ 0.8 × 109/L, SOFA score > 3, Acute Physiology and Chronic Health Evaluation II (APACHE II) score > 7, PaO2/FiO2 ≤ 200 mmHg, IL-6 > 120 pg/ml, and CRP > 52 mg/L.
Conclusions

Being critically ill and lymphocyte count, SOFA score, APACHE II score, PaO2/FiO2, IL-6, and CRP on admission were associated with poor prognosis in COVID-19 patients.
The value of lymph node ratio and total number of lymph nodes examined for resected pancreatic signet ring cell carcinoma: a retrospective cohort study
Authors: Ren Chao , Xue Feng , Wu Yinying , Wang Zheng 
Published: October 2020

Abstract
Background—Pancreatic signet ring cell carcinoma (SRCC) was an exceedingly rare histological subtype of pancreatic cancer. Previous studies focused on the trends of incidence and independent predictors of pancreatic SRCC. Our objectives of the study was to analyze the prognostic value of lymph node ratio (LNR) and explore the minimal number of lymph nodes examined to accurately evaluate the N stage in resected pancreatic signet ring cell carcinoma.Method—The data diagnosed from January 1, 1990 to December 31, 2016 constituted the study cohort from the Surveillance, Epidemiology, and End Results(SEER) registry. We calculated overall survival (OS) of these patients using Kaplan–Meier analysis and Cox proportional hazards model and used receiver-operating characteristic curve (ROC) analysis to investigate the discriminatory ability of the total number of lymph nodes examined(TNLE) relative to whether lymph node metastasis.Results—The median number of lymph nodes examined among 120 patients of resected pancreatic SRCC was 14 (interquartile range, 6.25 to 20.0).According to the univariate analysis of overall survival(OS) result, age, grade, chemotherapy, LNR and TNLE were significantly different(P<0.05).Multivariate survival analysis showed that LNR and grade were the independent prognostic indicators after pancreatic SRCC resection for OS. TNLE ≥ 8 showed the highest discriminatory power to evaluate whether the lymph node metastasis (AUC 0.656, 95%CI 0.564-0.741, Youden index 0.2533, sensitivity 78.67%, specificity 46.67%, P= 0.003)Conclusion—Our study indicated that LNR was a valuable independent prognostic factor for resected pancreatic SRCC. Regional lymphadenectomy of at least 8 lymph nodes was necessary to stage patients accurately. Enough number lymph nodes examined was necessary for the clinicians to accurately predict the significance of LNR in resected pancreatic SRCC.
Treatment of SARS-CoV-2 induced pneumonia with NAD+ in a mouse model
Authors: Jiang Yisheng , Deng Yongqiang , Ma Tiantian , Pang Huanhuan , Hu Zeping , Qin Cheng , Xu Zhiheng 
Published: October 2020

Abstract
The global COVID-19 epidemic has spread rapidly around the world and has already caused the death of more than one million people. As there is yet no vaccine, it is urgent to develop effective strategies to treat COVID-19 patients. Here, we used a mouse-adapted SARS-CoV-2 infection model to explore potential therapeutic targets for SARS-CoV-2 pneumonia. Global gene expression analysis of infected mouse lungs revealed dysregulation of genes associated with NAD+ metabolism, immune response and cell death, similar to that in COVID-19 patients. We therefore investigated the effect of treatment with NAD+ and found that the pneumonia phenotypes, including excessive inflammatory cell infiltration and embolization in SARS-CoV-2 infected lungs were significantly rescued by boosting NAD+ levels. Most notable, cell death was suppressed substantially (>65%) by NAD+ supplementation. Thus, our in vivo mouse study supports trials for treating COVID-19 patients with NAD+ or its precursors.
Whole Genome Sequencing in single CTC improves clinical outcome in Her-2 negative breast cancer patients
Authors: Li Yongping , Yuan Hao , Zhang Bin , Jiang Xiaofei , Yu Minghua , Zhu Hongbo , You Qinghua , Wang Ling , Yu Bo 
Published: October 2020

Abstract
Backgroud: Tumor tissues are usually highly heterogeneous and difficult to characterize which could mislead treatment strategy. Circulating tumor cells (CTCs) represent the most active and invasive tumor cells. This study retrospectively investigated the feasibility of individualized treatment of breast cancer patients based on genome sequencing of single cell CTC. Twenty-four CTCs were identified in three patients with breast cancer. For each patient, one polyploid CTC was captured and on which the whole genome sequencing (WGS) was performed. Based on the histopathological Her-2 status in tumor tissue and the HER2 gene status in WGS results of CTC, we adjusted treatment strategies, and monitored disease progression. Results: Patient ID1 and ID2 are with Her-2 protein overexpression in primary tumors and HER2 gene amplification in the DNA of CTCs. In patient ID3, histological examination of primary tumor and liver metastases revealed Her-2 negative, but the WGS analysis of CTC showed that the HER2 gene was amplified. After adjusting treatment by adding Her-2 inhibitors according to the results of CTC sequencing, the liver metastases and pleural effusion were significantly reduced 2 month later, CTC number and ctDNA burden were decreased, and 18-month progression-free survival (PFS) was recorded. In addition, some potential therapeutic targets and mutations in drug-resistant genes were found. Conclusions: The results of CTC sequencing effectively guided treatment of a patient with HER2 gene amplification in CTC but with Her-2 negative on tumor tissue. CTC sequencing is useful in resolving the heterogeneity of tumors and providing precision medicine for patients.
Percutaneous Transapical approach in the Treatment of Paravalvular Leak
Authors: Ruan Weiqiang , Yang Qin , Gan Changping , Lin Ke 
Published: October 2020

Abstract
Background: Paravalvular leaks (PVLs) occur in up to 17% of patients after valve replacement. Due to the high morbidity and mortality associated with re-operation, percutaneous transapical (TA) closure has been introduced as an alternative to the surgical repair and percutaneous transcatheter closure in the treatment of PVLs. Here, we evaluated the outcomes of patients who underwent TA-closure for PVLs at the our centre.Methods: This study analyzed records of 12 patients with 13 problematic mechanical valves who underwent treatment at our hospital from April 2015 to November 2018. All procedures were performed under general anesthesia, with preoperative CT reconstruction and guidance by three-dimensional transesophageal echocardiography (3D-TEE). Results: Our data showed that all the TA punctures were successful with delivery sheath sizes that ranged from 5-7 Fr. Out of the 13 valves, 10 were single leaks (76.9%) and were all closed by a single occluder. There were 2 double leaks (15.3%) which were closed by two single bigger devices, while the remaining double leak was corrected by two separate devices. The median duration of the procedure was 82 minutes (IQR, 65-85 minutes). In addition, the mean postoperative ICU stay was 4 days (IQR, 1-2 days) and mean duration from procedure to hospital discharge was 7 days (IQR, 4-7 days). One patient died prior to the discharge. Conclusion: Taken together, our data demonstrates that percutaneous TA is safe and is associated with a low complication rate and procedure-related mortality. Multiple leaks and diameter of ≥ 10 mm might increase the risk for residual regurgitation.
Spontaneous Conception Following Laparoscopic Surgery in Women with Infertility at the Yaoundé Gyneco-Obstetric and Pediatric Hospital: A Cross-Sectional and Analytical Study
Authors: Mokam Marie Noelle , Ndoua Cyrille Claude Noa , Djuikwo Felicite , Sandie Arsène Brunelle , Ojong Samuel , Foumane Pascal 
Published: October 2020

Abstract
Background: The infertility is a common health issue affecting many couples with a broad consequence on their lives. This study investigated the effectiveness of laparoscopic surgery for treating infertility in Cameroon.Methods: We conducted a cross-sectional, analytic study in order to determine the rate of conception and associated factors following laparoscopic surgery in women who presented with infertility, and treated at the Yaoundé Gynaeco-Obstetric and Pediatric Hospital from January 1st 2007 to December 31st 2017.Results: The average age of the included patients was 32.2 ± 4.9 years old. Married women and those with tertiary level studies were the most represented. The average duration of infertility stood at 4.6 ± 2.8 years. The most common surgical procedures were adhesiolysis (86.1%) and neo-salpingotomy (55.4%). The conception rate after the endoscopic surgery was 39.3% (119/303).Conclusions: The study revealed the efficiency of laparoscopic surgery in the management of infertility.
Artificial Intelligence for Reduced Dose 18F-FDG PET Examinations: A Real-World Deployment Through a Standardized Framework and Business Case Assessment
Authors: Penna Daniele , Katsari Katia , Arena Vincenzo , Polverari Giulia , Ianniello Annarita , Italiano Domenico , Milani Rolando , Roncacci Alessandro , Pelosi Ettore , Illing Rowland 
Published: October 2020

Abstract
BackgroundTo determine whether artificial intelligence (AI) processed PET/CT images of reduced by 33% administered 18-F FDG activity acquired in a single center, were non-inferior to native scans and if so to assess the potential impact of commercialization. MethodsSubtlePET™ AI was introduced in a PET/CT center in Italy. Eligible patients referred for 18F-FDG PET/CT were prospectively enrolled. Administered 18F-FDG was reduced to two-thirds of standard dose. Patients underwent one low-dose CT and two sequential PET scans; ‘PET-processed’ with reduced dose and standard acquisition time, and ‘PET-native’ with an elapsed time to simulate standard acquisition time and dose. PET-processed images were reconstructed using SubtlePET™. PET-native images were defined as the standard of reference. The datasets were anonymized and independently evaluated in random order by four blinded readers. The evaluation included subjective image quality (IQ) assessment, lesion detectability and assessment of business benefits. ResultsFrom February to April 2020, 61 patients were prospectively enrolled. Subjective IQ was not significantly different between datasets (4.62±0.23, p=0.237) for all scanner models, with ‘almost perfect’ inter-reader agreement. There was no significant difference between datasets in lesions’ detectability, target lesion mean SUVmax value and liver mean SUVmean value (182.75/181.75 [SD:0.71], 9.8/11.4 [SD:1.13], 2.1/1.9 [SD:0.14] respectively). No false-positive lesions were reported in PET-processed examinations. Agreed SubtlePET™ price per examination was 15-20% of FDG savings. ConclusionThis is the first real-world study to demonstrate the non-inferiority of AI processed 18F-FDG PET/CT examinations obtained with 66% standard dose and a methodology to define the AI solution price.