bluuebunny/crossref_metadata_2025_split huggingface dataset:
Renal Replacement Therapy for Burn Patients: A Systematic Review and Meta-Analysis
Authors: Duan ZhiYu , Chen FengKun , Cai GuangYan , Li JiJun , Chen XiangMei 
Published: September 2020

Abstract
Background: To improve the prognosis of burn patients with renal replacement therapy (RRT), we performed this systematic review and meta-analysis.Methods: We searched multiple databases for studies published before February 2020. Studies about adult populations with burn injury, providing epidemiologic data on prevalence or mortality of RRT, were included. Results: A total of selected 58 studies, including 38,787 patients were enrolled in our analysis. The prevalence rates of RRT were 5.14% (95%CI 4.54%-5.74%) in all burn patients and 35.8% (95%CI 29.54%-42.07%) in acute kidney injury (AKI) patients. The prevalence of RRT among burn patients in the intensive care unit (ICU) was 10.92% (95%CI 8.71%-13.14%). The mortality of all burn patients with RRT was 65.52% (95%CI 58.41%-72.64%). The mortality of patients with RRT in ICU was 62.7% (95%CI 53.7%-71.7%). The mortality rate of RRT patients was 30.33% (95%CI 22.06%-38.59%) of the total. There was no significant correlation (r=-0.224, P=0.159) between the year of publication and the mortality of burn patients with RRT. Neither cohort studies nor RCT studies of subgroup analyses show that RRT could reduce the risk of death in burn patients with AKI. Bleeding (10.92%) and secondary infection (9.61%) were the most common RRT-related adverse reactions. Compared with heparin, regional citrate anticoagulation has advantages in superior filter life spans and fewer bleeding episodes. Dialysis-requiring AKI in burn patients could increases the risk of chronic kidney disease progression and end-stage renal disease. About 35% of RRT patients need to maintain hemodialysis temporarily, even if they survive and leave hospital. Conclusions: The prevalence of RRT is not low; approximately, one-third of burn patients with AKI need RRT. The mortality of burn patients with RRT is very high and accounts for 1/3 of the total deaths. There is no evidence that RRT can improve the prognosis of burn patients with AKI. Regional citrate anticoagulation has some advantages in reducing bleeding and extending filter life spans, which may be more suitable for severe burn patients with CRRT.
Risk Factors For COVID-19 Positivity in Hospitalized Patients in A Low Prevalence Setting
Authors: Zohar Iris , Schwartz Orna , David Debby Ben , Mashavi Margarita , Aboulil Mohamad , Yossepowitch Orit , David Shirley Shapiro Ben , Maor‬‏ ‪Yasmin 
Published: September 2020

Abstract
Background: Identifying hospitalized patients with Coronavirus disease 2019 (COVID-19) in a low prevalence setting is challenging. We aimed to identify differences between COVID-19 positive and negative patients. Methods: Hospitalized patients with respiratory illness, or fever, were isolated in the emergency room and tested for COVID-19. Patients with a negative PCR and low probability for COVID-19 were taken out of isolation. Patients with a higher probability for COVID-19 remained in isolation during hospitalization and were retested after 48 hours. Risk factors for COVID-19 were assessed using logistic regression. Results: 254 patients were included, 37 COVID-19-positive (14.6%) and 217 COVID-19-negative (85.4%). Median age was 76 years, 52% were males. In a multivariate regression model, variables significantly associated with COVID-19 positivity were exposure to a confirmed COVID-19 case, length of symptoms before testing, bilateral and peripheral infiltrates in chest X-ray, neutrophil count within the normal range, and elevated LDH. In an analysis including only patients with pneumonia (N=78, 18 positive for COVID-19), only bilateral and peripheral infiltrates, normal neutrophil count and elevated LDH were associated with COVID-19 positivity. Conclusions: The clinical presentation of COVID-19 positive and negative patients is similar, but radiographic and laboratory features may help to identify COVID-19 positive patients and to initiate quick decisions regarding isolation.
Parathyroid Carcinoma with Sarcomatoid Differentiation: A Case Report and Literature review
Authors: Hu Liang , Xie Xiaojun 
Published: September 2020

Abstract
BackgroundParathyroid carcinoma (PC) is a rare thyroid tumor. PC with sarcomatoid differentiation(PCSD) is even rarer and its exact etiology remains unclear. We here report a case of PCSD, and present the clinicopathological features and pathological diagnosis and review the literature. Case presentationA 71-year-old man presented with a mass of 4.5 cm × 3.5 cm in the right neck. The tumor was composed of nest-like transparent cells, and the septum had heterotypic rhabdoid cells with sarcomatoid differentiation. Immunophenotype was as follows: MyoD1, myogenin and desmin were positive; clear cells were positive for CGA, Syn and GATA-3; and Ki-67 proliferation index was 40%. Hematoxylin and eosin staining and immunohistochemistry were performed. The patient was diagnosed with PCSD, and died 6 months after surgery.ConclusionsPCSD is a rare type of primary parathyroid tumor with high malignancy and poor prognosis. Definitive diagnosis should be based on histopathological morphology and immunophenotype, and surgical treatment should be performed as soon as possible.
Chronic Mental Health Impacts of Climate Change Extremes: A Case Study of the Largest Californian Wild Fire
Authors: Silveira Sarita , Kornbluh Mariah , Withers Mathew C. , Grennan Gillian , Ramanathan Veerabhadran , Mishra Jyoti 
Published: September 2020

Abstract
Background. Weather-related disasters such as droughts and fires as accelerated by climate change have led to substantial growth in interest in impacted health outcomes. While physical health outcomes have been studied in this context, our understanding of climate change impacted mental health is at its infancy. This study focuses on the mental health impacts of the largest Californian wildfire to-date, the Camp Fire of 2018.Methods. We investigated a sample of 780 Californian residents with different degrees of disaster exposure, and measured mental health using clinically valid scales for post-traumatic stress disorder (PTSD), major depressive disorder (MDD), and generalized anxiety disorder (GAD); data were collected six months post-exposure to understand sustained chronic impacts. Data were modeled using multiple-regression analyses. Additionally, we included vulnerability and resilience factors in hierarchical regression analyses.Results. Our primary finding is that direct exposure to large scale fires significantly increased the risk for all three mental health disorders, PTSD, MDD and GAD. Indirect exposure, for those who witnessed but did not personally experience the fires, increased the risk for MDD and GAD. Inclusion of vulnerability and resilience factors led to significantly improved prediction of all mental health outcomes. Low socio-economic status, childhood trauma and sleep disturbances were identified as vulnerability factors, while self-reported resilience had a positive effect on mental health. Mindfulness was associated with lower MDD and GAD symptom scores.Conclusion. Overall, our study demonstrates that climate-related extremes such as fires severely impact long-term mental wellbeing. Additionally, pre-existing adverse life events, resilient personality traits and lifestyle factors play an important role in the development of psychopathology after such disasters. Unchecked climate changes of magnitude projected for the latter half of this century risk severely impacting the mental wellbeing of the global population.
Relationship Among Atrial Fibrillation, CHADS2 Score and Ischaemic Stroke in Patients With Coronary Artery Disease: A Propensity Score Matching Study
Authors: Shi Boqun , Liu Demin , Wang Qian , Geng Xue , Hou Qian , Gu Guoqiang , Xie Ruiqin , Cui Wei 
Published: September 2020

Abstract
Objective: The purpose of this study was to investigate the relationship among atrial fibrillation (AF), CHADS2 score and ischaemic stroke in patients with coronary artery disease.Methods: A total of 2335 patients with coronary artery disease were included. They were divided into a nonischaemic stroke group (n=1997) and an ischaemic stroke group (n=388). Propensity score matching (PSM) was used to match ischaemic stroke patients with nonischaemic stroke patients in a 1:4 ratio. The relationship between AF, CHADS2 score and ischaemic stroke was evaluated using a univariate generalized linear model in different age, body mass index, and coronary artery disease subgroups. Univariate and multivariate generalized linear models were used to evaluate the relationship between AF and ischaemic stroke in different models.Results: Compared with the nonischaemic stroke group, the rate of AF (8.81% vs. 14.20%, P=0.002) in the ischaemic stroke group was higher. The proportion of patients with ischaemic stroke was significantly different between the AF group and the non-AF group (19.04% vs. 28.74%, P=0.003). With increasing CHADS2 score, the incidence of AF gradually increased (P for trend <0.001). With increasing AF burden, the rate of ischaemic stroke increased continuously (P for trend <0.001). Subgroup analysis showed that the trend towards increased stroke risk in the AF group was consistent across the various subgroups. Multivariate analysis demonstrated that paroxysmal and nonparoxysmal AF were not associated with ischaemic stroke compared with the absence of AF.Conclusion: After adjusting for confounding factors, the correlation between AF and ischaemic stroke decreased. Atherosclerotic factors may play an important role in ischaemic stroke in patients with coronary heart disease.
Enhanced Cell Survival and Therapeutic Benefits of IL-10-expressing Multipotent Mesenchymal Stromal Cells for Muscular Dystrophy
Authors: Kasahara Yuko Nitahara , Kuraoka Mutsuki , Oda Yuki , Kinoh Hiromi Hayashita , Takeda Shin’ichi , Okada Takashi 
Published: September 2020

Abstract
Background: Multipotent mesenchymal stromal cells (MSCs) are potentially therapeutic for muscle disease because they can accumulate at the sites of injury and are immunosuppressive. MSCs are attractive candidates for cell-based strategies that target diseases with chronic inflammation, such as Duchenne muscular disease (DMD). We focused on the IL-10 based on anti-inflammatory properties and hypothesized that IL-10 could increase the typically low survival of MSCs by exerting a paracrine effect after transplantation.Methods: We developed a continuous IL-10 expression system of MSCs using an adeno-associated virus (AAV) vector. To investigate the potential benefits of using AAV/IL-10 vector-transduced MSCs (IL-10-MSCs), we examined the cell survival rates in the skeletal muscles after intramuscular injection into mice and dogs. The systemic treatment of IL-10-MSCs derived from dental pulp (DPSCs) was comprehensive analyzed using the canine X-linked muscular dystrophy model in Japan (CXMDJ ), which has a severe phenotype similar to DMD patient. Results: In vivo bioluminescence imaging analysis revealed higher retention of IL-10-MSCs injected into the hind-limb muscle of mice. In the muscles of dogs, myofiber-like tissue was formed after the stable engraftment of IL-10-MSCs. Repeated systemic administration of IL-10-DPSCs into CXMDJ model resulted in long-term engraftment of cells, slightly increased serum levels of IL-10.IL-10-hDPSCs showed remarkably reduced the expression of pro-inflammatory MCP-1 and IL-6, and upregulated stromal-derived factor-1 (SDF-1). In fact, MRI and histopathology of the hDPSC-treated CXMDJ indicated the regulation of inflammation in the muscles, but not myogenic differentiation from treated cells. hDPSC-treated CXMDJ showed improved running capability, and recovery in tetanic force with concomitantincrease in physical activity. Serum creatine kinase levels, which increased immediately after exercise, were suppressed in the IL-10-hDPSC-treated CXMDJ . Conclusions: In case of local injection, IL-10-MSCs could maintain the long-term engraftment status and facilitate associated tissue repair. In case of repeated systemic administration, IL-10-MSCs facilitated the long-term retention of the cells in the skeletal muscleand also protected muscles with physical damage-induced injury, which improved muscle dysfunction in DMD. We can conclude that the local and systemic administration of IL-10-producing MSCs offers potential benefits for DMD therapy, through the beneficialIL-10 paracrine effects and may exert SDF-1.
CircNFIX promotes progression of pituitary adenoma via CCNB1 by sponging miR-34a -5p
Authors: Cheng Jianhua , Nie Ding , Li Bin , Gui SongBai , Li ChuZhong , Zhang YaZhuo , Zhao Peng 
Published: September 2020

Abstract
Background: Previous studies have shown that CCNB1 affects the invasiveness of pituitary adenomas, and it is of great significance to find the upstream mechanism of regulating CCNB1.Methods: RT-qPCR was used to measure the expression of circNFIX and miR-34a-5p in pituitary adenoma tissues. Western blotting was used to detect the expression of CCNB1 in pituitary adenomas. The relationship between circNFIX and miR-34a-5p was determined using dual-luciferase reporter assays. Pituitary adenoma cell invasion, migration and proliferation were analyzed using transwell, colony formation and CCK-8 assays, respectively. Additionally, xenograft experiments were performed to determine the effect of circNFIX silencing on tumor growth. Results: In pituitary adenoma tissues, the expression of circNFIX (has-circ_0005660) and CCNB1 were upregulated, while miR-34a-5p expression was downregulated. The silencing of circNFIX or overexpression of miR-34a-5p inhibited cell invasion, migration and proliferation. Inhibition of miR-34a-5p expression reversed the inhibitory effect of circNFIX silencing on the progression of pituitary adenoma. Conclusions: CircNFIX affects cell invasion, migration, and proliferation in pituitary adenomas by sponging miR-34a-5p through CCNB1. Therefore, circNFIX is expected to serve as a potential target for the treatment of pituitary adenomas.
Determinants of Unintended Pregnancies Among Currently Married Women in Uganda
Authors: Wasswa Ronald , Kabagenyi Allen , Atuhaire Leonard 
Published: September 2020

Abstract
Background: Unintended pregnancies are no longer bound to teenagers or school going children, married women in Uganda as well experience such pregnancies though little has been investigated on them. This study therefore examines the determinants of unintended pregnancies among currently married women in Uganda.Methods: In this study, we used data from the 2016 Uganda Demographic and Health Survey (UDHS) which comprised of 11,223 married women aged 15-49 years. Analysis was done using descriptive analysis, logistic regression, Poisson regression, log-rank test for survival functions, cox proportional hazards model, and the generalized structural equation model.Results: The study revealed that 45% of the pregnancies were unintended while 3 in 10 married women were not using contraceptives. At the bivariate level; unintended pregnancy was associated with the highest wealth quintile (OR=0.45, 95%CI=0.40-0.49) while contraceptive use was associated with higher education level (OR=4.90, 95% CI=4.10-5.86). Similarly, children ever born were associated with married women from rural areas (IRR=4.34, 95% CI=4.30-4.39). At the multivariate level, married women from northern region (AOR=0.55, 95% CI=0.45-0.64) had lower odds and Muslim married women with more children (AOR=1.04, 95% CI=1.01-1.07) had higher odds of unintended pregnancy.Conclusion: Unintended pregnancies are directly and indirectly influenced by higher fertility and improver use of contraceptive. Married women who had more children and were: from poor households, with lowest education, in Central region, in rural areas, with low age at first birth, with older partners and were Muslim were more likely to have unintended pregnancies. Also, married women who were using contraceptives and were: older age, Anglican, from wealthiest households, in agricultural or domestic sector and higher parity were associated with higher risk of unintended pregnancies. The government should make efforts in reducing the fertility among married women by investing in programs and policies like: sensitization of women on the effectiveness in use of contraceptives, making contraceptives affordable and easily accessible to all people in different regions of the country with emphasis on who already have four or more children. Extension of higher education to all people will lead to reduced risks of unintended pregnancies.
Multiregion sequencing reveals the genetic correlation of esophageal squamous cell carcinoma and matched cell-free DNA
Authors: Yuan Zuyang , Wang Xinfeng , Geng Xiao , Li Yin , Mu Juwei , Tan Fengwei , Xue Qi , Gao Shugeng , He Jie 
Published: September 2020

Abstract
Background: The aim of this study was to assess whether both ubiquitous and heterogeneous somatic mutations could be detected in circulating cell-free DNA (cfDNA) from patients with esophageal squamous cell carcinoma (ESCC). Methods: Paired multi-regional tumor tissues, cfDNA and white blood cells (WBCs) collected from five ESCC patients before treatment from a prospective study (NCT02395705). Of them, samples from Cohort 1 (E102 and E110) were sequenced by whole-exome sequencing (WES) and those from Cohort 2 (E104, E111 and E121) were sequenced by targeted captured sequencing with a panel of 560 cancer-related genes respectively. To call somatic single nucleotide variations (SNVs) by comparing the solid tumor or cfDNA with matched WBCs, the minimal variant allele frequency (VAFmin) as 0.1% and P value <0.05 were allowed. Results: Genomic DNA (gDNA) and plasma-derived cfDNA from 26 samples were successfully sequenced. In Cohort 1, 596 (596/712, 83%) and 562 (562/796, 71%) were heterogeneous SNVs in E102 and E110 respectively. There was a statistically significant linear relationship between the VAFs for tumor and cfDNA (R2 = 0.78, P <0.0001). In Cohort 2, 296 (296/323, 92%), 384 (384/423, 91%) and 331 (331/357, 93%) were heterogeneous SNVs in E104, E111 and E121respectively. cfDNA could recover an average of 60.7% (31/51; range, 35.7%-76.2%) of somatic mutations present in matched solid tumors. The correlation of VAFs between cfDNA and matched solid tumor was significantly positive (r2 =0.92, P <0.0001).Conclusions: Both sequencing approaches revealed the highly intratumoral heterogeneity in ESCC and enabled the detection of both ubiquitous and heterogeneous mutations in cfDNA. Further validation in cfDNA is required to define its potential utility for ESCC in clinical practice. Trial registrationAll patients selected in this study were from the registered clinical trial from ClinicalTrials.gov (NCT02395705). Date of registration: March 24, 2015.
Greyscale 2D nanograting fabrication by multistep nanoimprint lithography and ion beam etching
Authors: Buhl Janek , Yoo Danbi , Köpke Markus , Gerken Martina 
Published: September 2020

Abstract
The application of nanopatterned electrode materials is a promising method to improve the performance of thin-film optoelectronic devices such as organic light-emitting diodes and organic photovoltaics. Light coupling to active layers can be enhanced by employing individual nanopatterns specifically tailored to the device structure. During the development process typically a range of different nanopatterns need to be evaluated. Fabrication of each of these nanopatterns using electron-beam lithography is time and cost intensive, particularly for larger scale devices, due to the serial nature of electron beam writing. Here, we present a meth-od to generate nanopatterns of varying depth with different nanostructure designs from a single one-dimensional grating template structure with fixed grating depth. We employ multiple subsequent steps of UV nanoimprint lithography and ion beam etching to fabricate greyscale two-dimensional nanopatterns. After each imprint step, the imprint resist is cured and etched to maintain the structural conformity. In this work we present variable greyscale nanopatterning of the widely used electrode material indium tin oxide. We demonstrate the fabrication of periodic pillar-like nanostructures with different period lengths and heights in the two grating directions. The patterned films can be used either for immediate device fabrication or pattern reproduction by convention-al nanoimprint lithography. This parallel processing approach promises cost-efficient large-scale nanopattern variation for the device development process.
Diagnosis of Coronavirus disease by measuring serum concentrations of IL-6 and blood Ferritin
Authors: Yazdanpanah Parviz , Vafaei Farzad , Javdansirat Saeed , pouranfard Jalal , Afrouz Sajad 
Published: September 2020

Abstract
Objectives: Coronavirus disease 2019 (COVID-19) associated by infection and high death rate. The unresolved questions about the fatality rate of COVID-19 is most probably related to cytokine storm syndrome. There is currently no specific medication. Understanding the pathogenic pathway of this disease will lead to production of treatment and decreases of death rate. The aim of this study is to investigate changes of peripheral blood parameters (Interleukin-6 and Ferritin) in COVID-19 patients, which may be beneficial in the management of patients.Methods: In this case-control study, we collected data of 270 subjects in two groups including 133 patients with severe type COVID-19 (case) and 137 patients with nonsevere (control) between March 20 and May 21, 2020, and the clinical symptoms and inflammatory indications of patients diagnosed by laboratory test in Shahid Jalil hospital of Yasuj University of medical Sciences were collected to explore potential markers for disease monitoring. The data were analyzed by SPSS software version 20. Descriptive statistics, T-test and bivariate correlation tests were used to analyze. Results: The enrolled COVID-19 patients consisted of 53.4% males and 46.6% females with the medium age of 45.56±18.55 years and there were 50.04% males and 49.6% females with the medium age of 45.59±17.0 years for non COVID-19 patients. There was no significant difference in the age and sex ratio between two population under study. The proportion interstitial abnormalities evidenced by CT imaging in COVID-19 patients was 91.0%, while, 4.4% abnormalities was found in non COVID-19 patients. The frequency of positive RT-PCR test for case and control groups were 88.0% and 3.6%, respectively. The mean IL-6 and Ferritin levels and hematological parameters in two groups of patients with COVID-19 and non- COVID-19, were significantly different across all comparisons.There was a direct positively correlated between serum level of IL-6, Ferritin levels and hematological parameters including WBC, Lymphocytes, Neutrophils and Hb, except for platelets (negatively correlate), with COVID-19. Conclusions: In conclusion, inflammatory markers specifically IL-6 and Ferritin and hematological parameters (WBC, Lymphocytes, Neutrophils, Platelet and Hb) were correlated with the severity of COVID-19. Measurement of IL-6, Ferritin and hematological indices might be workable tests to diagnosis and prognosis of patients with COVID-19.