bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correction to: Functional exploration of co-expression networks identifies a nexus for modulating protein and citric acid titres in Aspergillus niger submerged culture
Authors: Cairns Timothy C. , Feurstein Claudia , Zheng Xiaomei , Zhang Li Hui , Zheng Ping , Sun Jibin , Meyer Vera 
Published: December 2019

Following publication of the original article [1], the authors provided revised images for Figs. 4, 6 and 7 which were not processed by the typesetter during proof-correction stage. The corrected figures are given with this erratum.
First report of Entamoeba moshkovskii in human stool samples from symptomatic and asymptomatic participants in Kenya
Authors: Kyany’a Cecilia , Eyase Fredrick , Odundo Elizabeth , Kipkirui Erick , Kipkemoi Nancy , Kirera Ronald , Philip Cliff , Ndonye Janet , Kirui Mary , Ombogo Abigael , Koech Margaret , Bulimo Wallace , Hulseberg Christine E. 
Published: December 2019

AbstractEntamoeba moshkovskii is a member of the Entamoeba complex and a colonizer of the human gut. We used nested polymerase chain reaction (PCR) to differentiate Entamoeba species in stool samples that had previously been screened by microscopy. Forty-six samples were tested, 23 of which had previously been identified as Entamoeba complex positive by microscopy. Of the 46 specimens tested, we identified nine (19.5%) as E. moshkovskii-positive. In seven of these nine E. moshkovskii-positive samples, either E. dispar or E. histolytica (or both) were also identified, suggesting that co-infections may be common. E. moshkovskii was also detected in both symptomatic and asymptomatic participants. To the best of our knowledge, this is the first report of E. moshkovskii in Kenya.
Adapting TeamSTEPPS for school mental health teams: a pilot study
Authors: Wolk Courtney Benjamin , Stewart Rebecca E. , Cronholm Peter , Eiraldi Ricardo , Salas Eduardo , Mandell David S. 
Published: December 2019

Abstract
Background
School mental health care often is provided by teams contracted from community mental health agencies. The team members that provide this care, however, do not typically receive training in how to work effectively in a team-based context. Team Strategies and Tools to Enhance Performance and Patient Safety (TeamSTEPPS) provides a promising, evidence-based strategy for improving communication and climate in school-based teams.

Methods
In collaboration with stakeholders, we adapted and piloted TeamSTEPPS for use with school mental health teams. Teams in six schools were randomized to receive the adapted TeamSTEPPS approach or usual supports. The main outcomes of interest were feasibility and acceptability of the adapted TeamSTEPPS strategy.

Results
Results indicated that team member burnout was significantly higher at follow-up than pretreatment for both control and intervention teams. TeamSTEPPS was feasible and acceptable to implement, and leadership emerged as an important facilitator. Barriers to implementation success included staff turnover, lack of resources, and challenges in the school mental health team relationship. Additional supports to implement TeamSTEPPS were suggested, including ongoing consultation and booster training to address high staff turnover.

Conclusions
Results suggest that TeamSTEPPS is promising for school mental health teams but additional modifications are likely needed.
What influences road mortality rates of eastern grey kangaroos in a semi-rural area?
Authors: Green-Barber Jai M. , Old Julie M. 
Published: December 2019

Abstract
Background
Roads have major ecological impacts on wildlife. Vehicle collisions most frequently impact large herbivores due to their larger home range compared to smaller animals, and higher population density compared to carnivores. Kangaroos (Macropus spp.) account for a large proportion of reported wildlife vehicle collisions that occur in N S W, Australia. We aimed to evaluate what influenced road mortality of eastern grey kangaroos (Macropus giganteus) in a temperate rural/suburban region. The location of roadkilled kangaroos found on or near two 1 km stretches of road in Richmond NSW was recorded throughout 2014 and 2015. Weather and moon phase data were recorded for the date of each roadkilled kangaroo. Transects were setup on both roads, and multiple road and landscape features, including the width of roadside, fence construction, habitat type, and distance from street lights measured at 50 m intervals. Data were analyzed to explore which landscape features and temporal factors influenced the occurrence of a roadkill hotspot.

Results
More kangaroo road mortalities occurred during periods of low temperature and low rainfall, and these factors are likely to affect forage quality. Fewer mortalities occurred when rain was falling. A greater number of mortalities occurred during the waning gibbous phase of the lunar cycle. Significantly more road mortalities occurred a short distance from the end of a section of street lights.

Conclusions
The findings suggest that illumination influences the likelihood of kangaroo road mortalities. Large herbivores are particularly sensitive to habitat fragmentation because they need unrestricted access to large continuous habitat. Knowledge of factors that influence where and when kangaroos are most likely to cross roads can be used to inform more targeted management strategies and improve future road design and habitat connectivity to reduce the incidence of wildlife vehicle collisions.
Impact of combine releases of the egg parasitoid, Trichogramma euproctidis (Girault) and the entomopathogenic nematode, Heterorhabditis bacteriophora to control Tuta absoluta (Meyrick) in tomato greenhouses in Egypt
Authors: Adly D. , Nouh Gehan M. 
Published: December 2019

AbstractIn greenhouses, tomato plants are subject to attack by several pest species. The present study aimed to investigate the compatibility of releasing the egg parasitoid, Trichogramma euproctidis (Girault) (Hymenoptera: Trichogrammatidae), and the entomopathogenic nematode, Heterorhabditis bacteriophora, strain HP88 against the tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), in commercial tomato greenhouses (Cherry and Bushra varieties) at winter plantation of 2018–2019 in Egypt. Three commercial plastic greenhouses were used. The first plastic greenhouses were treated by the two tested biological control agents (BCG), the second sprayed by certain recommended pesticides (PG), and the third used for control. Pheromone traps were used for monitoring the appearance of T. absoluta moths. The combined use of T. euproctidis and H. bacteriophora resulted to reduce the population density of the T. absoluta gradually until the end of the season in BCG. In the 17th week of treatments by T. euproctidis and H. bacteriophora, the population density of T. absoluta was estimated as leaf mines/plant (0.8 and 1.26 mines/leaf, in Cherry and Bushra varieties, respectively) in BCG. Also, in the 17th week of treatments by recommended pesticides, the population density of T. absoluta reached 12.73 mines/leaf and 18.33 mines/leaf, in CG. Early use of pesticides, by the appearance of T. absoluta infestation, could not suppress its population density that continued to increase until the end of the season in PG. Results revealed that the combination of the tested biological control agents against T. absoluta is recommended to be a main part in pest management practices in tomato greenhouses.
Stress hyperglycemia and electrolytes disturbance in patients with acute cerebrovascular stroke
Authors: El-Fawal Bastawy M. , Badry Reda , Abbas Wael A. , Ibrahim Ahmed K. 
Published: December 2019

Abstract
Background
Stroke is a medical emergency and considered the 2nd leading cause of mortality worldwide. High morbidity and mortality of stroke are due to some complications like cerebral edema, infection, and associated heart disease. Stroke may cause stress hyperglycemia and electrolyte disturbance that may be independent predictors for increased morbidity and mortality. Data on electrolyte disorder in neurological conditions especially acute stroke are somewhat scanty and not frequently interpreted.

Objectives
The current study aimed to evaluate the effect of stress induced by acute stroke on the blood glucose and serum electrolytes and its relationship to the type, severity, and prognosis of stroke.

Subjects and methods
A total of 100 patients admitted with acute stroke were included in the study. After informed consent, fasting blood sugar, glycosylated hemoglobin, serum sodium, and potassium levels were measured simultaneously. Stroke severity and outcome were assessed using the Glasgow Coma Scale.

Results
Stress hyperglycemia was noted in 21 patients, out of which 62% presented with hemorrhagic stroke. Undiagnosed diabetes was found in 47% of cases. Also, 13 % of our patients had both stress hyperglycemia and electrolyte disturbance simultaneously. Mortality among hemorrhagic versus ischemic patients was 46% and 22%, respectively. The independent predictors of mortality were stress hyperglycemia, electrolytes disturbance, ischemic heart diseases, and type and admission severity of stroke.

Conclusion
Stress hyperglycemia and electrolyte disturbance after acute stroke were found in 21% and 47% of patients respectively and may adversely affect outcome of stroke especially those with cerebral hemorrhage.
High survivorship of highly cross-linked polyethylene in revision Total hip Arthroplasty: a minimum 10-year follow-up study
Authors: Lim Seung-Jae , Yeo Ingwon , Park Chan-Woo , Lee Kyung-Jae , Min Byung-Woo , Park Youn-Soo 
Published: December 2019

Abstract
Purpose
Highly cross-linked polyethylene has been introduced to decrease osteolysis secondary to polyethylene wear debris generation. However, few long-term data on revision total hip arthroplasty (THA) using highly cross-linked polyethylene liners are available. The objective of this study was to determine long-term outcomes of a highly cross-linked polyethylene liner in revision THA.

Materials & methods
We evaluated 63 revision THAs performed in 63 patients using a highly cross-linked polyethylene liner between April 2000 and February 2005. Of these, nine died and four were lost to follow-up. Thus, the final study cohort consisted of 50 patients (50 hips), including 26 males and 24 females with a mean age of 53 years (range, 27–75 years). Mean follow-up was 11 years (range, 10–14 years).

Results
The mean Harris hip score improved from 44 points preoperatively to 85 points at the final follow-up. No radiographic evidence of osteolysis was found in any hip. The mean rate of polyethylene liner wear was 0.029 mm/year (range, 0.003 to 0.098 mm/year). A total of 5 hips (10%) required re-revision arthroplasty, including one cup loosening, one recurrent dislocation, and three deep infections. Kaplan-Meier survivorship with an end point of re-revision for any reason was 91.1% and for aseptic cup loosening was 97.9% at 11 years.

Conclusion
At a minimum of 10 years, the highly cross-linked polyethylene liners showed excellent clinical performance and implant survivorship, and were not associated with osteolysis in our patients with revision THAs.
MRI for assessment of pathologic nipple discharge: is it mandatory?
Authors: Zaky Mona M. , Hafez Asmaa , Zaky Marwa M. , Shoma Ashraf , Soliman Nermin Y. , Elmokadem Ali H. 
Published: December 2019

Abstract
Background
Benign breast lesions is the most common cause of nipple discharge; however, a rare but major cause is breast cancer. This study assesses the superadded value of MRI in diagnosing causes of pathologic nipple discharge. Ninety-three patients with pathologic nipple discharge were evaluated by sonomammography and DCE-MRI. Sonomammography and MR imaging features were analyzed and correlated with the histopathology.

Results
Histopathology revealed 69 benign, three high-risk, and 21 malignant lesions. Simply dilated ducts and presence of a mass on US examination as well as non-mass enhancement and STIR signal changes on MRI were of statistically significant probability in differentiation between benign and malignant causes of pathological nipple discharge (p value = 0.017 and 0.001) and (p value ≤ 0.001). Sensitivity and specificity of mammogram and ultrasound in differentiation between benign and malignant causes of pathologic nipple discharge were 71.4% and 54.2% respectively with positive predictive value of 31.2%,negative predictive value of 86.7%, and accuracy of 58.1%. MRI gave higher sensitivity and specificity of 100% and 83.3% with positive predictive value of 63.6%, negative predictive value of 100%, and accuracy of 87.1%.

Conclusion
Magnetic resonance imaging is superior to sonommagraphy in diagnosis of pathologic nipple discharge and we recommend it in special situations.
Proton MR spectroscopy in characterization of focal bone lesions of peripheral skeleton
Authors: Amar Meenu , Ghasi Rohini Gupta , Krishna L. G. , Khanna Geetika 
Published: December 2019

Abstract
Background
The aim of our study was to determine the value of single-voxel proton MR spectroscopy (1HMRS) in distinguishing benign from malignant focal bone lesions in the peripheral skeleton. MRI and 1HMRS was performed in 50 focal lesions (> 1 cm size) detected on radiographs of peripheral skeleton.1HMRS was performed at 1.5 T with TE of 144 ms with automatic shimming and water suppression. Qualitative analysis for a discrete choline peak at 3.2 ppm was done. Significance of the presence of choline peak on 1HMRS in distinguishing benign from malignant lesions was calculated using histopathology as a gold standard. Chi-square test was used and p value < 0.05 was considered significant.

Results
Forty-one benign and 9 malignant lesions were confirmed by histopathological results. Amongst malignant lesions, choline peak was positive in all but 1 case of low-grade lymphoma. MR spectra of 11 benign lesions showed the presence of choline peak. All 7 benign giant cell tumors (GCT) were positive for choline peak. The sensitivity, specificity, PPV, NPV of proton MR spectroscopy in differentiating benign from malignant lesions were 87.5%,71%,38.8%, and 96.4% respectively. p value was significant (< 0.05).

Conclusion
1HMRS in focal bone lesions can help in the differentiation of malignant from benign musculoskeletal tumors. Although some benign lesions may show false-positive result, absence of choline peak is a reliable reassurance against malignancy. GCT is an exception amongst benign bone tumors as it consistently shows the presence of choline peak on 1HMRS.
Propranolol therapy in infantile hemangioma: correlation of age and duration of treatment to the outcomes
Authors: Al-Mayoof Ali Farooq , Joda Ali Egab , Almushhadany Osama Esmaeel 
Published: December 2019

Abstract
Background
Infantile hemangioma (IH) is the commonest vascular tumor affecting children that appears in the first 2 weeks of life and follows a proliferative phase that continues during the first year of life. After then, it undergoes involution, which lasts for several months or years depending on the size, site, gender, and development of complications. The purpose of this prospective study was to evaluate the correlation of age and duration of propranolol therapy to the outcomes of infantile hemangioma.

Methods
A prospective study included 28 patients with IH in which the propranolol therapy was initiated in a dose of 3 mg/kg/day divided into two to three doses. The surface area of IH was calculated monthly using AutoCAD software. Treatment with propranolol was discontinued when there was no more decrease in the surface area for two consecutive visits. Eleven males and 17 females completed the study. The age at initiation of therapy ranged from 2 to 16 months while at the end of therapy it was 9 to 23 months.

Results
The mean difference percent of surface area at 6 months was (51.1 ± 16.3), while at the end of the treatment course was (75.0±16.8) which was statistically significant (P < 0.0001). In addition, a significant inverse correlation was found between the age at the beginning of treatment and the difference percent of surface area. A similar inverse correlation was observed between the age at the beginning of treatment and the duration of treatment.

Conclusion
In addition to the safety and efficacy of propranolol therapy for IH, a higher response rate can be gained with early treatment and a prolonged course of therapy. In addition, propranolol therapy should be continued until there is no more response for two consecutive months regardless of the age of therapy initiation and the duration of treatment.
Quantifying koala locomotion strategies: implications for the evolution of arborealism in marsupials
Authors: Gaschk Joshua L. , Frère Celine H. , Clemente Christofer J. 
Published: December 2019

ABSTRACTThe morphology and locomotor performance of a species can determine their inherent fitness within a habitat type. Koalas have an unusual morphology for marsupials, with several key adaptations suggested to increase stability in arboreal environments. We quantified the kinematics of their movement over ground and along narrow arboreal trackways to determine the extent to which their locomotion resembled that of primates, occupying similar niches, or basal marsupials from which they evolved. On the ground, the locomotion of koalas resembled a combination of marsupial behaviours and primate-like mechanics. For example, their fastest strides were bounding type gaits with a top speed of 2.78 m s−1 (mean 1.20 m s−1), resembling marsupials, while the relatively longer stride length was reflective of primate locomotion. Speed was increased using equal modification of stride length and frequency. On narrow substrates, koalas took longer but slower strides (mean 0.42 m s−1), adopting diagonally coupled gaits including both lateral and diagonal sequence gaits, the latter being a strategy distinctive among arboreal primates. The use of diagonally coupled gaits in the arboreal environment is likely only possible because of the unique gripping hand morphology of both the fore and hind feet of koalas. These results suggest that during ground locomotion, they use marsupial-like strategies but alternate to primate-like strategies when moving amongst branches, maximising stability in these environments. The locomotion strategies of koalas provide key insights into an independent evolutionary branch for an arboreal specialist, highlighting how locomotor strategies can convergently evolve between distant lineages.
Selective Oxidative Hydroxylation of Arylboronic Acids by Colloidal Nanogold Catalyzed In Situ Generation of H2O2 from Alcohols under Aerobic Conditions
Authors: Vinsen  , Uetake Yuta , Sakurai Hidehiro 
Published: December 2019

Abstract
Selective hydroxylation of arylboronic acids was achieved through PVP (polyvinylpyrrolidone)-stabilized nanogold catalyzed in situ generated H2O2 formed by the oxidation of an alcoholic solvent under aerobic conditions. The synthetic application of in situ generated H2O2 was investigated through aerobic epoxidation of (E)-chalcone.
Inter-fraction robustness of intensity-modulated proton therapy in the post-operative treatment of oropharyngeal and oral cavity squamous cell carcinomas
Authors: Hague Christina , Aznar Marianne , Dong Lei , Fotouhi-Ghiam Alireza , Lee Lip Wai , Li Taoran , Lin Alexander , Lowe Matthew , Lukens John N , McPartlin Andrew , O'Reilly Shannon , Slevin Nick , Swisher-Mcclure Samuel , Thomson David , Van Herk Marcel , West Catharine , Zou Wei , Teo Boon-Keng Kevin 
Published: December 2019

Objective:
To evaluate dosimetric consequences of inter-fraction setup variation and anatomical changes in patients receiving multifield optimised (MFO) intensity modulated proton therapy for post-operative oropharyngeal (OPC) and oral cavity (OCC) cancers.

Methods:
Six patients receiving MFO for post-operative OPC and OCC were evaluated. Plans were robustly optimised to clinical target volumes (CTVs) using 3 mm setup and 3.5% range uncertainty. Weekly online cone beam CT (CBCT) were performed. Planning CT was deformed to the CBCT to create virtual CTs (vCTs) on which the planned dose was recalculated. vCT plan robustness was evaluated using a setup uncertainty of 1.5 mm and range uncertainty of 3.5%. Target coverage, D95%, and hotspots, D0.03cc, were evaluated for each uncertainty along with the vCT-calculated nominal plan. Mean dose to organs at risk (OARs) for the vCT-calculated nominal plan and relative % change in weight from baseline were evaluated.

Results:
Robustly optimised plans in post-operative OPC and OCC patients are robust against inter-fraction setup variations and range uncertainty. D0.03cc in the vCT-calculated nominal plans were clinically acceptable across all plans. Across all patients D95% in the vCT-calculated nominal treatment plan was at least 100% of the prescribed dose. No patients lost ≥10% weight from baseline. Mean dose to the OARs and max dose to the spinal cord remained within tolerance.

Conclusion:
MFO plans in post-operative OPC and OCC patients are robust to inter-fraction uncertainties in setup and range when evaluated over multiple CT scans without compromising OAR mean dose.

Advances in knowledge:
This is the first paper to evaluate inter-fraction MFO plan robustness in post-operative head and neck treatment.
Glycogen storage diseases-time to flip the outdated diagnostic approach centered on liver biopsy with the molecular testing
Authors: Ahmed Sibtain , Afroze Bushra 
Published: December 2019

The glycogen storage diseases (GSDs) are a group of inherited metabolic disorders that result from a defect in any one of several enzymes required for either glycogen synthesis or glycogen degradation. The traditional diagnostic approach is based on the invasive hepatic or muscle biopsies, which are neither cost effective nor convenient. Molecular (gene testing) has emerged over the course of past few years as a robust alternative diagnostic tool, which not only confirms the diagnosis of GSDs but also clearly differentiates the types of GSDs allowing the initiation of the type-specific appropriate treatment for the particular type of GSDs. The aim of this update is to highlight the limitations of undertaking a liver biopsy for the diagnosis of GSDs; and to further describe the pros of the molecular testing for better patient centered care.
doi: https://doi.org/10.12669/pjms.36.2.1310
How to cite this:Ahmed S, Afroze B. Glycogen storage diseases-time to flip the outdated diagnostic approach centered on liver biopsy with the molecular testing. Pak J Med Sci. 2020;36(2):290-292. doi: https://doi.org/10.12669/pjms.36.2.1310
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.