bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome Analysis of Cinnamomum Migao Seed Germination in Medicinal Plants of Southwest China
Authors: Huang xiaolong , Tian Tian , Chen Jingzhong , Wang Deng , Tong Bingli , Liu Jiming 
Published: December 2020

Abstract
Background: Cinnamomum migao is an endangered evergreen woody plant species endemic to China. Its fruit is used as a traditional medicine by the Miao nationality of China and has a high commercial value. However, its seed germination rate is very low under natural and artificial conditions. As the foundation of plant propagation, seed germination involves a series of physiological, cellular, and molecular changes; however, the molecular events and systematic changes occurring during C. migao seed germination remain unclear.Results: In this study, combined with the changes in physiological indexes and transcription levels, we revealed the regulation characteristic of cell structures, storage substances, and antioxidant capacity during seed germination. Anatomical analysis revealed that abundant smooth and full oil bodies were present in the cotyledons of the seeds. With seed germination, the oil bodies and other substances gradually degraded to supply energy; this was consistent with the content of storage substances. In parallel to anatomical and physiological analyses, transcriptome analysis showed that 80%–90% of differentially expressed genes (DEGs) appeared after seed imbibition, reflecting important development and physiological changes. The unigenes involved in material metabolism (glycerolipid metabolism, fatty acid degradation, and starch and sucrose metabolism) and energy supply pathways (pentose phosphate pathway, glycolysis pathway, pyruvate metabolism, tricarboxylic acid cycle, and oxidative phosphorylation) were differentially expressed in the four germination stages. Among these DEGs, a small number of genes in the energy supply pathway at the initial stage of germination maintained high level of expression to maintain seed vigor and germination ability. Then, the genes involved in lipid metabolism were activated at a large scale, followed by the activation of the genes involved in CHO metabolism, which had its own species specificity. Conclusions: Our study revealed the transcriptional levels of genes during seed germination as well as determined the order of the metabolic pathways. The changes in cell structure and physiological indexes also confirmed these events. Our findings lay a foundation for determining the molecular mechanisms underlying seed germination.
A functional variant in the RAD51 3′ UTR is associated with survival of hepatocellular carcinoma patients
Authors: Qiu Moqin , Liu Yingchun , Lin Qiuling , Jiang Yanji , Zhou Zihan , Wen Qiuping , Liang Xiumei , Huo Rongrui , Zhou Xianguo , Yu Hongping 
Published: December 2020

Abstract
The RAD51 gene plays an important role in DNA repair by homologous recombination, and is involved in the development and progression of multiple cancers. While single nucleotide polymorphisms in RAD51 have been previously described to impact patient prognosis, it is still unclear whether this is also true for hepatocellular carcinoma (HCC). This study therefore aimed to identify genetic variants in RAD51 and determine the effect of these variants on the survival of patients with HCC. In this study, we performed genotyping assays for RAD51 polymorphisms in a cohort of 368 patients with HCC who had undergone radical surgery resection. Using multivariate cox proportional hazards model and Kaplan-Meier analyses with log-rank tests, we compared the survival of patients with HCC according to RAD51 SNP genotypes. We performed expression quantitative trait loci (eQTL) analysis to investigate correlations between SNP rs12593359 and RAD51 mRNA expression levels from the genotype-tissue expression portal. We identified one potential functional variant, rs12593359, located in a microRNA (miRNA) binding site in the RAD51 3′ untranslated region, to be an independent predictor of overall survival of patients with HCC in the dominant model. Patients carrying GT/TT genotypes had a significantly increased risk of death when compared with those carrying GG genotype (adjusted hazard ratio = 1.34, 95% confidence interval = 1.02–1.76, P = 0.035). Kaplan-Meier curve analysis showed a markedly shorter survival time for patients with HCC carrying GT/TT genotypes of SNP rs12593359 (19.0 months vs. 36.0 months; P log−rank = 0.012). Notably, this effect was particularly pronounced in several subgroups of patients (e.g., males, Hepatitis B virus-positive patients, patients with a single tumor nodule, patients with alpha-fetoprotein (AFP) < 400 ng/ml, or patients who were cancer embolus-free). Additional expression analysis of quantitative trait loci showed that the rs12593359 was significantly associated with RAD51 mRNA expression levels in 483 cell-cultured fibroblasts (P = 1.1 × 10− 4). These findings provide evidence that RAD51 rs12593359 is associated with HCC survival and may serve as a promising predictor of survival in patients with HCC.
Prognostic Nomogram for Patients With Unresectable Pancreatic Cancer Treated With Gemcitabine Plus Nab-paclitaxel or FOLFIRINOX: Real-world Results From a Multicenter Retrospective Study (NAPOLEON study).
Authors: Shibuki Taro , Mizuta Toshihiko , Shimokawa Mototsugu , Koga Futa , Ueda Yujiro , Nakazawa Junichi , Komori Azusa , Otsu Satoshi , Arima Shiho , Fukahori Masaru , Makiyama Akitaka , Taguchi Hiroki , Honda Takuya , Mitsugi Kenji , Nio Kenta , Ide Yasushi , Ureshino Norio , Shirakawa Tsuyoshi , Otsuka Taiga 
Published: December 2020

Abstract
Background No reliable nomogram has been developed until date for predicting the survival in patients with unresectable pancreatic cancer undergoing treatment with gemcitabine plus nab-paclitaxel (GnP) or FOLFIRINOX (FFX).Methods This analysis was conducted using clinical data of patients with unresectable pancreatic cancer undergoing GnP or FFX treatment obtained from a multicenter study (NAPOLEON study). A Cox proportional hazards model was used to identify the independent prognostic factors. A nomogram to predict 6-, 12-, and 18-month survival probabilities was generated, validated by using the concordance index (C-index), and calibrated by the bootstrapping method. And then, we attempted risk stratification for survival by classifying the patients according to the sum of the scores on the nomogram (total nomogram points; TNP).Results A total of 318 patients were enrolled. A prognostic nomogram was generated using data on the Eastern Cooperative Oncology Group performance status, liver metastasis, serum LDH, serum CRP, and serum CA19-9. The C-indexes of the nomogram were 0.77, 0.72 and 0.70 for 6-, 12-, and 18-month survival, respectively. The calibration plot showed optimal agreement at all points. Risk stratification based on tertiles of the TNP yielded clear separations of the survival curves. The median survival times in the low-, moderate-, and high-risk groups were 15.8, 12.8 and 7.8 months (P<0.05), respectively.Conclusions: Our nomogram is a convenient and inexpensive tool to accurately predict survival in patients with unresectable pancreatic cancer undergoing treatment with GnP or FFX, and will help clinicians in selecting appropriate therapeutic strategies for individualized management.
An ancient motif unique for human STING, RGS12 and SARS-CoV-2 spike proteins
Authors: Aydin Suleyman , Aydin Ayca Cakmak 
Published: December 2020

Abstract
The coronavirus named as SARS-CoV-2 is the cause of the COVID-19 pandemic and spreading rapidly1. It is a pneumonia outbreak2 with T cell exhaustion, cytokine storm and coagulation3,4. Short motifs on proteins play important roles on protein-protein interactions5,6. We hypothetized role of molecular mimicry of small-xxx-small motifs for the spike protein of SARS-CoV-2. Here we show that a unique and evolutionary conserved motif is found only on the spike protein of SARS-CoV-2 and stimulator of interferon genes (STING) proteins. Surprisingly we could not find this motif on any other protein of any living form. We found a similar, but not identical motif mimicry for the spike and regulator of G protein signaling 12 (RGS12), C1QT4 and also for proteins of Archaea and beta-lactamase enzymes of bacteria including Mycobacterium tuberculosis. STING proteins have roles on coagulation, T cell exhaustion, cytokine release1-3 and RGS12 on inflammation7. In contrast to cGAS-STING pathway8, the motif mimicry indicated a direct interaction between spike and STING proteins suggesting the importance of STING, RGS12 and C1QT4 on the pathogenesis of COVID-19. To our surprise, the molecular mimicry showed that beta-lactamase inhbitors may be effective against SARS-CoV-2. The motif is unique, as found on Archaea and Cnidaria it is evolutionary old but a new target and mechanism for the COVID-19.
A Decade of Plague in Madagascar: A Description of Two Hot Spot Districts
Authors: Rakotosamimanana Sitraka , Kassie Daouda , Taglioni François , Ramamonjisoa Josélyne , Rakotomanana Fanjasoa , Rajerison Minoarisoa 
Published: December 2020

Abstract
Background Human plague cases, mainly the bubonic form, occur annually in the endemic regions of the central highlands of Madagascar. The aim of the study is to compare the evolution of the epidemiological features of human plague in two districts of the central highlands.Methods In Madagascar, all clinically suspected plague cases that meet clinical and epidemiological criteria specified in the World health organization standard case definition are reported to the national surveillance system. Data on plague cases reported between 2006 and 2015 in the districts of Ambositra and Tsiroanomandidy were analyzed. Statistical comparisons between the epidemiological characteristics of the two districts were conducted. Results A total of 840 cases were reported over the period studied, including 563 (67%) probable and confirmed cases (C+P). Of these C+P cases, nearly 91% (488/536) were cases of bubonic plague. Reported clinical forms of plague were significant for both districts during 2006 to 2015 (p=0.001). Plague cases occurred annually in a period of 10 years in Tsiroanomandidy district. One year of absence of cases characterized Ambositra district during the same period. Conclusion The differences in the epidemiological situation with respect to the plague from 2006 to 2015 in the two central highland districts may suggest that several factors other than biogeographical factors determine the expression of the plague and its evolution in this region. The need to take into account epidemiological situations according to the specific contexts of the districts could improve results in the fight against the plague in Madagascar.
Relationship Between Undernutrition and Periodontal Diseases Among Yemen Population: a Cross-sectional Study
Authors: Al-kalisi Milad , Al-Hajri Manal , Al-Rai Sarah 
Published: December 2020

Abstract
Background: Undernutrition is an inadequate supply of energy and nutrients. Periodontal diseases (PDs) defined as a broad form of chronic inflammatory diseases of the gingiva, bone and ligaments supporting the teeth. This study aimed to reveal the effect of undernutrition, using body mass index (BMI) and serum albumin level (Alb) on PDs and other risk factors as age, smoking and khat chewing. Methods: This was a cross-sectional study conducted at the faculty of dentistry, Sana’a University. Of 1920 patients attended to clinics, only 229 matched the study criteria. Oral examination was performed to assess the periodontal clinical parameters measurements. BMI and Alb was measured. Statistical analysis was used to present the association between categorical variables was assessed using Chi square test and Fisher-Exact test. ANOVA was used to assess the differences in the mean values of the quantitative outcomes. Chi square test was used to evaluate the association between BMI and age, gender, occupation, education level, smoking, khat chewing as well as BMI with PDs. Results: Most of participants (58.5%) were males and most of the study sample (91.3%) was at the age group of (18-35). Among all subjects, (81.2%) of cases were diagnosed with gingivitis. (60.7%) of study participants were mildly undernourished according to BMI. (93%) of participants showed normal Alb level. Regarding to habits, only (18.2%) of patients were smokers and more than half of participants (59.4%) were khat chewers. Conclusion: There was a relationship between PDs and undernutrition which was obviously seen between gingivitis and mild undernutrition.
The GAINS trial - Growth and Anabolism In Intensive care Survivors
Authors: Anstey Matthew , Rauniyar Rashmi , Fitzclarence Ethan , Tran Ngan , Osnain Emma , Mammana Bianca , Jacques Angela , Palmer Robert N , Chapman Andrew , Wibrow Bradley 
Published: December 2020

Abstract
BackgroundTo explore the feasibility, safety and potential benefits from administration of nandrolone to patients in the recovery phase from critical illness weakness.MethodsIn this phase 2 randomized controlled trial, adult critically ill patients admitted for longer than 7 days with significant weakness, received nandrolone ((males 200 mg, females 100 mg) or placebo weekly, up to a total of three doses. Primary outcome measures were improvement in grip strength, medical research council (MRC) muscle strength sum score, and functional activity level (Chelsea critical care assessment tool (CPAx)).Results22 patients were enrolled between September 2017 and May 2019. No significant adverse events were detected. Median grip strength change was non-significantly greater in the nandrolone group (left hand (9.9 vs 4.4, p = 0.190), right (5.8 vs 3.0, p = 0.343)). The discharge CPAx and ICU mobility scores scores were higher in the nandrolone group, although there was no difference in the change in CPAx score (16.4 vs 17.2, p = 0.865). There were no changes in ultrasound detected muscle thickness between groups.ConclusionsIn patients with prolonged critical illness, nandrolone appears to be safe but a larger study, potentially combined with resistance exercise, is needed to definitively address the potential benefits.Trial registration: ANZCTR registration number : ACTRN12616000835448. Registered 27/6/2016.https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=370920
Identification of Metal Pollutants in Ambient Air (Case Study: West Surabaya, East Java, Indonesia)
Authors: Ciptaningayu Tresta Nurina , Syafei Arie Dipareza , Santoso Muhayatun , Hermana Joni , Boedisantoso Rachmat , Assomadi Abdu Fadli 
Published: December 2020

Abstract
Surabaya City, especially the western area of Surabaya, is utilized for industrial space, warehousing, final disposal sites (FDS), and port areas. Activities in these areas produce emissions, particularly PM2.5 (particulate matter smaller than 2.5 µm) and PM10 (particulate matter smaller than 10 µm). PM2.5 and PM2.5−10 contain metals and can cause respiratory disorders and death. Therefore, it is necessary to determine the concentrations of PM2.5 and PM2.5−10 and the heavy metals contained in the PMs. Sampling for PM2.5 and PM2.5−10 was conducted using a Gent Stacked Filter Unit (Gent SFU) every six (6) days between October 2019–April 2020. The samples were then analyzed using gravimetry to determine mass concentrations of PM2.5 and PM10 and analyzed using XRF (X-Ray Fluorescence) to determine the elements contained within PM2.5 and PM2.5−10 and their concentrations. The elemental concentrations were used as Positive Matrix Factorization (PMF) inputs to identify potential pollutant sources. The results of the PMF analysis were then used to determine the source locations of pollutants by using a Conditional Probability Function (CPF). The observed concentrations of PM2.5 and PM10 are 11.47 µg/m3 and 27.49 µg/m3, or 11.45 µg/Nm3 and 26.98 µg/Nm3. 18 elements are identified in the PM, namely Na, Mg, Al, Si, S, K, Cl, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, Br, and Pb. Potential sources of PM2.5 pollutants are a combination of Cu industries, biomass burning, Ni industries, non-ferrous metal industries, transportation activities, iron and steel industries, construction activities, dust, port activities, and Pb industries. Potential sources of PM2.5−10 pollutants are construction activities, dust, transportation activities, non- ferrous metals industries, and Ni industries. The estimated source locations of the pollutants contributing to PM2.5 and PM2.5−10 are spread out from north to southwest. Based on this analysis, the concentrations of PM2.5, PM2.5−10, and metal elements met air quality standards.
Genotype-Phenotype correlation of 17 cases of Pompe Disease in Spanish Patients and Identification of 4 Novel Mutations
Authors: Hernandez-Arevalo Paula , Santotoribio Jose D. , Delarosa-Rodriguez Rocio , Gonzalez-Meneses Antonio , Garcia-Morillo Salvador , Jimenez-Arriscado Pilar , Guerrero juan M , Macher Hada C. 
Published: December 2020

Abstract
Background: Pompe disease (PD) is an autosomal recessive metabolic disorder caused by mutations in the acid α-glucosidase gene (GAA) that produces defects in the lysosomal acid α-1,4-glucosidase. We aimed to identify genetic variations and clinical features in Spanish subjects to establish genotype-phenotype correlation.Methods: A total of 2709 subjects who showed symptoms or susceptible signs of PD were enrolled in this observational study. Enzymatic activity was detected by fluorometric techniques and the genetic study was carried out using Next-Generation Sequencing.Results: Fourteen different variants from seventeen patients were identified, four of whom had not been described in the literature previously, including a homozygous variant. In all of them α-glucosidase activity was decreased. Muscle weakness, respiratory distress, exercise intolerance, hypotonia, dysphagia and myalgia were commonly observed in patients. Conclusions: This study report four new mutations that contribute to the mutation spectrum of the GAA gene. We confirm that patients in Spain have a characteristic profile of a European population, with c.-32-13T> G being the most prevalent variant. Furthermore, it was confirmed that the c.236_246delCCACACAGTGC mutation in homozygosis is associated with early disease and a worse prognosis.
Process evaluation of the implementation of a parenting programme for Somali-born parents living in Sweden
Authors: Osman Fatumo , Schön Ulla-Karin , Klingberg-Allvin Marie , Flacking Renée , Tistad Malin 
Published: December 2020

Abstract
Background: Forced migration has been shown to have a negative impact on family dynamics, parenting and the parent-child relationship. Parental support programmes aim to strengthen family functioning and the parent-child relationship and to promote the mental health of children and parents. However, there is a lack of knowledge on how parenting support programmes can be implemented for newly arrived immigrant parents. This process evaluation, therefore, describes the implementation of a successful parenting programme for immigrant parents from Somalia and identifies key components in the implementation process.Method: This process evaluation considered context, implementation and mechanism of impact, in accordance with the Medical Research Councils guidance. Data were collected through focus group discussions, a questionnaire, attendance lists, field and reflection notes, and observations of the sessions. The data were then analysed using content analysis and descriptive statistics. Results: The findings showed that of the 60 parents who were invited to the parenting programme, 58 participated in the sessions. The study showed that involving key individuals in the early stage of the implementation facilitated reaching the Somali-born parents in the parenting programme. To retain the participants in the programme, parents were offered free transportation. The programme was implemented and delivered as intended. A majority of the parents were satisfied with the programme, and they reported increased knowledge about children’s rights and the support they might seek from social services. Conclusions: This study illustrates how a parenting support programme can be implemented for Somali-born parents and provides guidance on how to engage and attract immigrant parents to participate in parenting support programmes.
Descriptions of two new species of Tarachoptera from Burmese amber (Insecta, Amphiesmenoptera: Tarachoptera)
Authors: Mey Wolfram , Wichard Wilfried , Müller Patrick , Wang Bo 
Published: December 2020

During examination of new material of Burmese amber three individuals of Tarachoptera were discovered. The inclusions are sufficiently well preserved and allow identification and description of these rare, fossil insects. The species belong to the family Tarachocelidae and are described as Kinitocelis macroptera spec. nov. (†) and Retortocelis spicipalpia spec. nov. (†). The species and their preservations are described in detail, and photos and line drawings are provided for wing venation, head, mouthparts, and abdomen. The hitherto described species of Tarachoptera are summarized in a checklist.

Taxonomic acts
Kinitocelis macroptera Mey & Wichard, spec. nov. – urn:lsid:zoobank.org:act:E0B1624A-C69E-4B53-851F-4368AC21C614Retortocelis spicipalpia Mey & Wichard, spec. nov. – urn:lsid:zoobank.org:act:9778E7E2-EEFE-4DC5-B891-54DF114D525C
„Die Blicke gehen alle nach vorne“ – Qualitative Rekonstruktion zum Anerkennungserleben von Bildungsfachkräften für Inklusion
Authors: Otten Matthias , Hempel Sebastian , Masurek Martina , Platte Andrea 
Published: December 2020

Die praxeologisch-rekonstruktiv ausgerichtete Erforschung des Bildungskontextes inklusiver Hochschule steht, anders als dies für den Bereich der rekonstruktiven Schul- und Unterrichts­forschung gesagt werden kann, noch am Anfang. Das in diesem Artikel vorgestellte Verbundprojekt erweitert den Blick auf Inklusionsbedingungen auf der Ebene der Lehre von Bildungsfachkräften. Damit löst es sich von einer sonst dominierenden Adressat*innenlogik, welche die Inklusionsfrage fast ausschließlich für die Lernenden stellt. Die im Projekt durchgeführten Videografien und Gruppendiskussionen wurden auf Grundlage der Dokumen­tarischen Methode analysiert. Der vorliegende Artikel geht auf das Forschungsdesign und erste Forschungsergebnisse ein. Hierbei spielt das rekonstruierte Anerkennungserleben der Bildungsfachkräfte eine zentrale Rolle.Abstract
Compared with the fast growing amount of reconstructive studies on inclusion in school research, reconstructive studies on inclusive teaching at universities are rare. First results of a reconstructive research project about inclusive teaching in higher and adult education widens the perspective on conditions of inclusion: It focusses on the teaching-praxis of so called ‘Bildungsfachkräfte’, people with learning disabilities who accomplished a training program lasting three years to qualify for teaching in various academic settings. Our analysis considers the layer of inclusive teaching instead of conditions of inclusive learning. The videographies and group discussions conducted in the project are analysed on the basis of the documentary method. The experiences of recognition will be analysed empirically as a pattern of orientation among the so called ‘Bildungsfachkräfte’ which guides their teaching approach towards inclusion.