bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ambulatory searching task reveals importance of somatosensation for lower-limb amputees
Authors: Christie Breanne P. , Charkhkar Hamid , Shell Courtney E. , Burant Christopher J. , Tyler Dustin J. , Triolo Ronald J. 
Published: June 2020

AbstractThe contribution of somatosensation to locomotor deficits in below-knee amputees (BKAs) has not been fully explored. Unilateral disruption of plantar sensation causes able-bodied individuals to adopt locomotor characteristics that resemble those of unilateral BKAs, suggesting that restoring somatosensation may improve locomotion for amputees. In prior studies, we demonstrated that electrically stimulating the residual nerves of amputees elicited somatosensory percepts that were felt as occurring in the missing foot. Subsequently, we developed a sensory neuroprosthesis that modulated stimulation-evoked sensation in response to interactions between the prosthesis and the environment. To characterize the impact of the sensory neuroprosthesis on locomotion, we created a novel ambulatory searching task. The task involved walking on a horizontal ladder while blindfolded, which engaged plantar sensation while minimizing visual compensation. We first compared the performance of six BKAs to 14 able-bodied controls. Able-bodied individuals demonstrated higher foot placement accuracy than BKAs, indicating that the ladder test was sensitive enough to detect locomotor deficits. When three of the original six BKAs used the sensory neuroprosthesis, the tradeoff between speed and accuracy significantly improved for two of them. This study advanced our understanding of how cutaneous plantar sensation can be used to acquire action-related information during challenging locomotor tasks.
Short-term exposure to air pollution (PM2.5) induces hypothalamic inflammation, and long-term leads to leptin resistance and obesity via Tlr4/Ikbke in mice
Authors: Campolim Clara Machado , Weissmann Lais , Ferreira Clílton Kraüss de Oliveira , Zordão Olivia Pizetta , Dornellas Ana Paula Segantine , de Castro Gisele , Zanotto Tamires Marques , Boico Vitor Ferreira , Quaresma Paula Gabriele Fernandes , Lima Raquel Patrícia Ataíde , Donato Jose , Veras Mariana Matera , Saldiva Paulo Hilário Nascimento , Kim Young-Bum , Prada Patricia Oliveira 
Published: June 2020

AbstractA previous study demonstrated that a high-fat diet (HFD), administered for one-three-days, induces hypothalamic inflammation before obesity’s established, and the long term affects leptin signaling/action due to inflammation. We investigate whether exposure to particulate matter of a diameter of ≤2.5 μm (PM2.5) in mice fed with a chow diet leads to similar metabolic effects caused by high-fat feeding. Compared to the filtered air group (FA), one-day-exposure-PM2.5 did not affect adiposity. However, five-days-exposure-PM2.5 increased hypothalamic microglia density, toll-like-receptor-4 (Tlr4), and the inhibitor-NF-kappa-B-kinase-epsilon (Ikbke) expression. Concurrently, fat mass, food intake (FI), and ucp1 expression in brown adipose tissue were also increased. Besides, decreased hypothalamic STAT3-phosphorylation and Pomc expression were found after twelve-weeks-exposure-PM2.5. These were accompanied by increased FI and lower energy expenditure (EE), leading to obesity, along with increased leptin and insulin levels and HOMA. Mechanistically, the deletion of Tlr4 or knockdown of the Ikbke gene in the hypothalamus was sufficient to reverse the metabolic outcomes of twelve-weeks-exposure-PM2.5. These data demonstrated that short-term exposure-PM2.5 increases hypothalamic inflammation, similar to a HFD. Long-term exposure-PM2.5 is even worse, leading to leptin resistance, hyperphagia, and decreased EE. These effects are most likely due to chronic hypothalamic inflammation, which is regulated by Tlr4 and Ikbke signaling.
Allele-specific expression is widespread in Bos indicus muscle and affects meat quality candidate genes
Authors: de Souza Marcela Maria , Zerlotini Adhemar , Rocha Marina Ibelli Pereira , Bruscadin Jennifer Jessica , Diniz Wellison Jarles da Silva , Cardoso Tainã Figueiredo , Cesar Aline Silva Mello , Afonso Juliana , Andrade Bruno Gabriel Nascimento , Mudadu Mauricio de Alvarenga , Mokry Fabiana Barichello , Tizioto Polyana Cristine , de Oliveira Priscila Silva Neubern , Niciura Simone Cristina Méo , Coutinho Luiz Lehmann , Regitano Luciana Correia de Almeida 
Published: June 2020

AbstractDifferences between the expression of the two alleles of a gene are known as allele-specific expression (ASE), a common event in the transcriptome of mammals. Despite ASE being a source of phenotypic variation, its occurrence and effects on genetic prediction of economically relevant traits are still unexplored in bovines. Furthermore, as ASE events are likely driven by cis-regulatory mutations, scanning them throughout the bovine genome represents a significant step to elucidate the mechanisms underlying gene expression regulation. To address this question in a Bos indicus population, we built the ASE profile of the skeletal muscle tissue of 190 Nelore steers, using RNA sequencing data and SNPs genotypes from the Illumina BovineHD BeadChip (770 K bp). After quality control, 820 SNPs showed at least one sample with ASE. These SNPs were widespread among all autosomal chromosomes, being 32.01% found in 3′UTR and 31.41% in coding regions. We observed a considerable variation of ASE profile among individuals, which highlighted the need for biological replicates in ASE studies. Functional analysis revealed that ASE genes play critical biological functions in the development and maintenance of muscle tissue. Additionally, some of these genes were previously reported as associated with beef production and quality traits in livestock, thus indicating a possible source of bias on genomic predictions for these traits.
Melatonin induces a stimulatory action on the scrotal skin components of Soay ram in the non-breeding season
Authors: Mustafa Fatma El-Zahraa A. , Abdel-maksoud Fatma M. , Hassan A. H. S. , Mokhtar Doaa M. 
Published: June 2020

AbstractFifteen adult Soay rams were employed in this study to investigate the effect of melatonin on the scrotal skin using histological, histochemical, and morphometrical analysis. The results revealed that the melatonin treated group showed a significant increase in the thickness of the epidermis, the cross-sectional area of blood capillaries and nerve fibers compared with the control one. In addition, obvious hypertrophy and hyperplasia were detected in the sebaceous glands in association with a significant increase in the number and diameter of apocrine sweat glands with well-developed secretory activity. S100 protein and cytokeratin-19 strongly stained the basal cells of sebaceous glands in the melatonin treated group incomparable to the control group. Moreover, the nerve fibers were intensively immunoreacted for S100 and cytokeratin proteins in the melatonin treated group in contrast to the control one. A high number of telocytes (TCs) could be identified in the treated group around the nerve fibers and blood vessels in the dermis. The number of Langerhans cells showed a significant increase in the melatonin groups that were identified by MHC II and PGP 9.5 within the epidermal layer. Furthermore, a significant increase in the number of dendritic cells was identified in the melatonin group, which were distributed within the dermis, around hair follicles, sebaceous glands, and sweat glands and were strongly expressed PGP-9.5, MHC-II, VAMP, SNAP, keratin-5, and cytokeratin-19 immunoreactivity. Notably, Merkel cells showed a significant increase in the number in the melatonin group that could be stained against nestin, SNAP, and VAMP. On the other hand, the secretory granules in sweat glands were exhibited a strong positive reactivity for synaptophysin in melatonin group. The current study showed that the administration of melatonin induced a stimulatory effect on keratinocytes, non-keratinocytes, sebaceous and sweat glands, hair follicles, as well as the vascular, neuronal, and cellular constituents of the dermis.
Monolayered Platinum Nanoparticles as Efficient Electrocatalysts for the Mass Production of Electrolyzed Hydrogen Water
Authors: Wang Yanqing , Fugetsu Bunshi , Sakata Ichiro , Fujisue Chika , Kabayama Shigeru , Tahara Norio , Morisawa Shinkatsu 
Published: June 2020

AbstractHigh-performance/low-cost platinum (Pt)-based electrocatalysts have been established by top-coating both sides of a titanium plate with Pt nanoparticles. The average diameter of the Pt nanoparticles used in this study is approximately 100 nm. Three types of Pt top-coated Pt/Ti electrocatalysts, each having different top-coated Pt layer thicknesses, are prepared. Type I is a monolayered Pt top-coated type, in which the thickness of the top-coated Pt layer is approximately 100 nm; Type II is a few-layered type with a top-coated Pt layer thickness of approximately 200 nm, and Type III is a multilayered type with a top-coated Pt layer thickness of approximately 750 nm. The mass loading of Pt is 0.0215 mg cm−2 for Type I, 0.043 mg cm−2 for Type II, and 0.161 mg cm−2 for Type III. The electrocatalytic activities of each type of Pt/Ti electrocatalyst are evaluated through the electrolysis of acidic water and tap water. Type I gives the highest electrocatalytic efficiencies, which are comparable or even better than the electrocatalytic efficiencies of the state-of-the-art commercially available Pt/C electrode and other metal-/carbon-based HER catalysts. For example, in the case of the electrolysis of acidic water at an overpotential of 0.15 V, Type I shows a Tafel slope of 29 mV dec−1 and a current density of 27.5 mA cm−2. Even in the case of the electrolysis of tap water, Type I gives an HER Faradaic efficiency of 92%. A model of water (H2O), hydronium ions (H3O+), and hydroxyl ions (OH−) properly adsorbing on the Pt (111) facet is proposed to explain the electrocatalytic mechanism. New insights into the distinguishing properties of the resultant electrolyzed hydrogen water (EHW), namely, the healthy beneficial effects of EHW, are also described, and a new concept of storing and carrying reductive hydrogen (H*) by free Pt nanoparticles is proposed.
An optimized test bolus for computed tomography pulmonary angiography and its application at 80 kV with 10 ml contrast agent
Authors: Wu Huiming , Chen Xiao , Zhou Hao , Qin Bin , Cao Jian , Pan Zhaochun , Wang Zhongqiu 
Published: June 2020

AbstractComputed tomography pulmonary angiography (CTPA) is usually used for pulmonary embolism (PE) detection. However, the determination of scan timing remains a challenge due to the short scan duration of CTPA. We aimed to develop an optimized test bolus to determine scan delay in CTPA. The time-enhancement curves were obtained by measuring the enhancement within a region of interest in the main pulmonary artery and vein. A total of 70 patients were randomly divided into two groups (n = 35 each): the control group underwent CTPA using the test bolus approach and the test group underwent CTPA using the biphasic time-enhancement curves approach. Tube voltages of 100 kVp and 80 kVp and 20 ml and 10 ml contrast agent were adopted in the control and test groups, respectively. The CT numbers, image quality, PE detection was evaluated. There was a point of intersection between the pulmonary artery and vein test bolus enhancement curves. The scan delay time (TDELAY) was obtained based on the time at intersection (TCROSS) and the scan duration (TSD): TDELAY = TCROSS − TSD. The mean CT numbers for pulmonary vein in the control were higher than those in the test group (all p < 0.001). The image quality for the pulmonary arteries in the test group was better than that in the control group (p < 0.01), with artifact reduction in the superior vena cava. Segmental PE could be detected using the optimized protocol. The radiation dose and iodine load in the test group were all lower than those in the control (p < 0.01). We established an approach to calculate the scan delay of CTPA, and this approach could be used for CTPA at 80 kVp with 10 ml contrast agent.
Cardiac MRI in common marmosets revealing age-dependency of cardiac function
Authors: Moussavi Amir , Mietsch Matthias , Drummer Charis , Behr Rüdiger , Mylius Judith , Boretius Susann 
Published: June 2020

AbstractThe aim of this study was to establish a feasible and robust magnetic resonance imaging protocol for the quantitative assessment of cardiac function in marmosets and to present normal values of cardiac function across different ages from young adult, middle-aged, to very old clinically healthy animals. Cardiac MRI of 33 anesthetized marmosets at the age of 2–15 years was performed at 9.4 T using IntraGate-FLASH that operates without any ECG-triggering and breath holding. Normalized to post-mortem heart weight, the left ventricular end-diastolic volume (LV-EDV) was significantly reduced in older marmosets. The LV end-systolic volume (LV-ESV) and the LV stroke volume (LV-SV) showed a similar trend while the LV ejection fraction (LV-EF) and wall thickening remained unchanged. Similar observations were made for the right ventricle. Moreover, the total ventricular myocardial volume was lower in older monkeys while no significant difference in heart weight was found. In conclusion, IntraGate-FLASH allowed for quantification of left ventricular cardiac function but seems to underestimate the volumes of the right ventricle. Although less strong and without significant sex differences, the observed age related changes were similar to previously reported findings in humans supporting marmosets as a model system for age related cardiovascular human diseases.
Quantitative Analysis of the Cerebral Vasculature on Magnetic Resonance Angiography
Authors: Goswami Pulak , Markey Mia K. , Warach Steven J. , Dula Adrienne N. 
Published: June 2020

AbstractThe arterial connections in the Circle of Willis are a central source of collateral blood flow and play an important role in pathologies such as stroke and mental illness. Analysis of the Circle of Willis and its variants can shed light on optimal methods of diagnosis, treatment planning, surgery, and quantification of outcomes. We developed an automated, standardized, objective, and high-throughput approach for categorizing and quantifying the Circle of Willis vascular anatomy using magnetic resonance angiography images. This automated algorithm for processing of MRA images isolates and automatically identifies key features of the cerebral vasculature such as branching of the internal intracranial internal carotid artery and the basilar artery. Subsequently, physical features of the segments of the anterior cerebral artery were acquired on a sample and intra-patient comparisons were made. We demonstrate the feasibility of using our approach to automatically classify important structures of the Circle of Willis and extract biomarkers from cerebrovasculature. Automated image analysis can provide clinically-relevant vascular features such as aplastic arteries, stenosis, aneurysms, and vessel caliper for endovascular procedures. The developed algorithm could facilitate clinical studies by supporting high-throughput automated analysis of the cerebral vasculature.
Pervasive Hydrothermal Events Associated with Large Igneous Provinces Documented by the Columbia River Basaltic Province
Authors: Bindeman I. N , Greber N. D. , Melnik O. E. , Artyomova A. S. , Utkin I. S. , Karlstrom L. , Colón D. P. 
Published: June 2020

AbstractThe degree and extent of crustal hydrothermal alteration related to the eruption of large igneous provinces is poorly known and not easily recognizable in the field. We here report a new δ18O dataset for dikes and lavas from the Columbia River Basalt Group (16–15 Ma) in the western USA, and document that dikes on average are 1–2‰ more depleted in δ18O than basalt flows. We show that this observation is best explained with the involvement of heated meteoric waters during their cooling in the crust. The largest 6–8‰ depletion is found around and inside a 10 m-thick feeder dike that intruded the 125 Ma Wallowa tonalitic batholith. This dike likely operated as a magma conduit for 4–7 years, based on the extent of heating and melting its host rocks. We show that this dike also created a hydrothermal system around its contacts extending up to 100 m into the surrounding bedrock. A model that considers (a) hydrothermal circulation around the dike, (b) magma flow and (c) oxygen isotope exchange rates, suggests that the hydrothermal system operated for ~150 years after the cessation of magma flow. In agreement with a previously published (U-Th)/He thermochronology profile, our model shows that rocks 100 m away from such a dike can be hydrothermally altered. Collectively, our sample set is the first documentation of the widespread hydrothermal alteration of the shallow crust caused by the intrusion of dikes and sills of the Columbia River Basalt Province. It is estimated that heating and hydrothermal alteration of sediments rich in organic matter and carbonates around the dikes and sills releases 18 Gt of greenhouse gases (CH4 and CO2). Furthermore, hydrothermal δ18O depletion of rocks around dikes covers 500–600 km3, which, when scaled to the total CRB province constitutes 31,000 km3 of low-δ18O rocks. These volumes of crust depleted in δ18O are sufficient to explain the abundant low-δ18O magmas in eastern Oregon and western Idaho. This work also demonstrates that the width and magnitude of δ18O depletion around dikes can identify them as feeders. Given this, we here interpret Paleoproterozoic dikes in Karelia with the world’s lowest δ18O depletions (−27.8‰) as feeders to the coeval large igneous province aged 2.2–2.4 Ga that operated under the Snowball Earth glaciation conditions.
Genome-wide identification and characterization of UDP-glucose dehydrogenase family genes in moso bamboo and functional analysis of PeUGDH4 in hemicellulose synthesis
Authors: Yang Ying , Kang Lan , Wu Ruihua , Chen Yuzhen , Lu Cunfu 
Published: June 2020

AbstractUridine diphosphate glucose dehydrogenases (UGDHs) are critical for synthesizing many nucleotide sugars and help promote the carbohydrate metabolism related to cell wall synthesis. In plants, UGDHs are encoded by a small gene family. Genome-wide analyses of these genes have been conducted in Glycine max and Arabidopsis thaliana, however, the UGDH gene family has not been comprehensively and systematically investigated in moso bamboo (Phyllostachys edulis), which is a special woody grass monocotyledonous species. In this study, we identified nine putative PeUGDH genes. Furthermore, analysis of gene duplication events and divergences revealed that the expansion of the PeUGDH family was mainly due to segmental and tandem duplications approximately 4.76–83.16 million years ago. An examination of tissue-specific PeUGDH expression indicated that more than 77% of the genes were predominantly expressed in the stem. Based on relative expression levels among PeUGDH members in different tissues in moso bamboo, PeUGDH4 was selected for detailed analysis. The results of subcellular localization indicated that PeUGDH4-GFP fusion proteins was observed to be localized in the cytoplasm. The ectopic overexpression of PeUGDH4 in Arabidopsis significantly increased the contents of hemicellulose and soluble sugar, suggesting that PeUGDH4 acts as a key enzyme involved in bamboo cell wall synthesis.
High yield of protein-rich forage rice achieved by soil amendment with composted sewage sludge and topdressing with treated wastewater
Authors: Phung Luc Duc , Ichikawa Megumi , Pham Dung Viet , Sasaki Atsushi , Watanabe Toru 
Published: June 2020

AbstractAiming to promote low-cost production of protein-rich forage rice and resource recycling from wastewater treatment plants, a pot experiment was conducted to assess the possibility to substitute mineral fertilizers with composted sewage sludge (CSS) with/without top-dressing with treated municipal wastewater (TWW). Results indicated that a basal application of CSS at 2.6 g N pot−1 replaced conventional mineral fertilization of 1.3 g N pot−1 to produce comparable yields with the same rice protein content, although there might be a risk of increased As concentration in rice grains. Interestingly, CSS application at a reasonable dose of 1.3 g N pot−1, followed by a topdressing with TWW resulted in 27% higher yield and 25% superior rice protein content relative to the mineral fertilization, with no risk of heavy metal(loid) accumulation in grains and in paddy soils. Here we demonstrated an appealing fertilization practice with zero use of mineral fertilizers in paddy rice cultivation, expectedly contributing towards sustainable rice farming and animal husbandry in Japan.
The origin of sheep settlement in Western Mediterranean
Authors: Kandoussi Asmae , Boujenane Ismaïl , Auger Clément , Serranito Bruno , Germot Agnès , Piro Mohammed , Maftah Abderrahman , Badaoui Bouabid , Petit Daniel 
Published: June 2020

AbstractThe arrival of Neolithic culture in North Africa, especially domestic animals has been essentially documented from archaeological records. As the data relative to sheep are scarce, we studied the genetic relationship between Moroccan sheep breeds and Mediterranean ones using the sequencing of 628 bp of the mitochondrial DNA control region in 193 Moroccan individuals, belonging to six breeds, and 652 sequences from other breeds in Europe and Middle East. Through Network analysis and an original phylogenetically derived method, the connection proportions of each Moroccan breed to foreign ones were estimated, highlighting the strong links between Moroccan and Iberian breeds. The first founders of Moroccan sheep population were issued at 79% from Iberia and 21% from a territory between Middle East and Africa. Their calculated expansion times were respectively 7,100 and 8,600 years B.P. This suggests that Neolithization was introduced by a double influence, from Iberia and from another route, maybe Oriental or Sub-Saharan. The consequence of the environmental changes encountered by founders from Iberia was tested using different neutrality tests. There are significant selection signatures at the level of Moroccan and European breeds settled in elevated altitudes, and an erosion of nucleotide diversity in Moroccan breeds living in arid areas.
Neuronal On- and Off-type heterogeneities improve population coding of envelope signals in the presence of stimulus-induced noise
Authors: Hofmann Volker , Chacron Maurice J. 
Published: June 2020

AbstractUnderstanding the mechanisms by which neuronal population activity gives rise to perception and behavior remains a central question in systems neuroscience. Such understanding is complicated by the fact that natural stimuli often have complex structure. Here we investigated how heterogeneities within a sensory neuron population influence the coding of a noisy stimulus waveform (i.e., the noise) and its behaviorally relevant envelope signal (i.e., the signal). We found that On- and Off-type neurons displayed more heterogeneities in their responses to the noise than in their responses to the signal. These differences in heterogeneities had important consequences when quantifying response similarity between pairs of neurons. Indeed, the larger response heterogeneity displayed by On- and Off-type neurons made their pairwise responses to the noise on average more independent than when instead considering pairs of On-type or Off-type neurons. Such relative independence allowed for better averaging out of the noise response when pooling neural activities in a mixed-type (i.e., On- and Off-type) than for same-type (i.e., only On-type or only Off-type), thereby leading to greater information transmission about the signal. Our results thus reveal a function for the combined activities of On- and Off-type neurons towards improving information transmission of envelope stimuli at the population level. Our results will likely generalize because natural stimuli across modalities are characterized by a stimulus waveform whose envelope varies independently as well as because On- and Off-type neurons are observed across systems and species.