bluuebunny/crossref_metadata_2025_split huggingface dataset:
Theoretical Consideration on the Application of Continuous Wavelet Transform to Time-Series Processing for Magnetotelluric Data
Authors: Ogawa Hiroki , Hama Yuki , Asamori Koichi , Ueda Takumi 
Published: September 2020

Abstract
In the magnetotelluric (MT) method, the responses of the natural electromagnetic fields are evaluated by transforming time-series data into spectral data and calculating the apparent resistivity and phase. The continuous wavelet transform (CWT) can be an alternative to the short-time Fourier transform, and the applicability of CWT to MT data has been reported. There are, however, few cases of considering the effect of numerical errors derived from spectral transform on MT data processing. In general, it is desirable to adopt a window function narrow in the time domain for higher-frequency components and one in the frequency domain for lower-frequency components. In conducting the short-time Fourier transform, because the size of the window function is fixed unless the time-series data are decimated, there might be difference between the calculated MT responses and the true ones due to the numerical errors. Meanwhile, CWT can strike a balance between the resolution of the time and frequency domains by magnifying or reducing the wavelet, according to the value of frequency. Although the types of wavelet functions and their parameters influence the resolution of time and frequency, those calculation settings of CWT are often determined empirically. In this study, focusing on the frequency band between 0.001 Hz and 10 Hz, we demonstrated the superiority of utilizing CWT in MT data processing and determined its proper calculation settings in terms of restraining the numerical errors caused by the spectral transform of time-series data. The results obtained with the short-time Fourier transform accompanied with gradual decimation of the time-series data, called cascade decimation, were compared with those of CWT. The shape of the wavelet was changed by using different types of wavelet functions or their parameters, and the respective results of data processing were compared. Through these experiments, this study indicates that CWT with the complex Morlet function with its wavelet parameter k set to 6 ≤ k < 10 will be effective in restraining the numerical errors caused by the spectral transform.
Autoantibodies Against Human Renal Glomerular Endothelial Cells in Lupus Nephritis That Induce Endothelial Interferon-Alpha Production
Authors: Hu Ya-Chiao , Tsai I-Jung , Hsu Hui-Yao , Chiang Bor-Luen , Yang Yao-Hsu 
Published: September 2020

Abstract
Background: The pathogenesis of lupus nephritis (LN) remains not fully understood. In this study, we aimed to explore the pathogenic roles of autoantibodies against human renal glomerular endothelial cells (HRGEC) in LN patients.Methods: The serum levels of anti-HRGEC antibodies in systemic lupus erythematosus (SLE) patients without LN and LN patients were determined by cell-based enzyme-linked immunosorbent assay (ELISA). Monoclonal IgG anti-HRGEC antibodies were subsequently generated from LN patients. The binding activities of these monoclonal antibodies to HRGEC, their cross-reactivity with double-stranded DNA (dsDNA), and the ability to activate HRGEC were further evaluated. Results: LN patients had higher serum levels of IgG anti-HRGEC antibodies than SLE patients without LN and healthy controls. Four monoclonal IgG anti-HRGEC antibodies (LN1-4) were obtained; LN1 and LN2 were IgG3 while LN3 and LN4 were IgG1. Among these monoclonal antibodies, LN1-3 were cross-reactive with dsDNA. The functional assays showed that compared with IgG1/IgG3 isotype controls, LN3 had the effect on HRGEC to enhance interleukin (IL)-6 production, LN4 could enhance IL-8 and monocyte chemoattractant protein (MCP)-1 production, and LN1-3 possessed the ability to induce interferon (IFN)-α production by HRGEC. Moreover, the removal of DNA on HRGEC surface by DNAse 1 did not interpose the binding of LN1-3 to HRGEC and the effects of LN1-3 on IFN-α induction by HRGEC.Conclusions: Some IgG anti-HRGEC antibodies in LN patients had the ability to enhance endothelial proinflammatory cytokines (IL-6, IL-8, and MCP-1) production and some could induce the DNA-independent production of IFN-α by HRGEC.
Assembly Building Material Management Method Based on BIM Technology
Authors: Wu Xinhua 
Published: September 2020

Abstract
The primary goal of material management is to reasonably arrange the use of materials, which is particularly important for the construction industry. In the process of material management, on the premise of meeting the requirements of production and construction materials, try not to increase additional inventory and reduce the amount of capital. In order to explore the method of prefabricated building material management, this paper uses the method of sampling survey and sand table simulation technology to compare the prefabricated building material management based on BIM Technology with the conventional method. We make a comparative study on the procurement, warehouse and material quality of materials, mainly focusing on the production stage and construction stage of the building, and analyze the difference of material management between the two technical methods in different stages. Through experiments, we find that the average inventory turnover times of the BIM based prefabricated building materials management method is 6, and that of the conventional method is 4, but in the construction stage, the gap between the two is narrowed. The turnover times of the prefabricated building material management method based on BIM Technology is reduced to 3, and that of the conventional method is reduced to 2. It can be seen that the prefabricated building material management based on BIM Technology can reduce the capital use and warehouse occupancy rate more than the conventional method, and at the same time, the quality control of materials is also better.
Sexual Dimorphism in Inorganic Mercury Toxicokinetics and the Attendant Lipotoxic and Non-Lipotoxic Dyslipidemia in the Rat
Authors: Wusu Adedoja Dorcas , Ogunrinola Olabisi Oluwafunmilayo , Afolabi Olusegun Kayode , Abam Esther Omugha , Babayemi David Oluwagbemi , Dosumu Oluwatosin Adebisi , Onunkwor Okechukwu Beno , Balogun Elizabeth Abidemi , Odukoya Olusegun Olukayode , ADEMUYIWA OLADIPO 
Published: September 2020

Abstract
BackgroundThe influence of variability in the biology of living organisms is poorly appreciated in toxicology. However, multiple lines of evidence indicate that sex-differences modulate toxicokinetics and toxicodynamics from cellular/molecular to whole animal levels resulting in different toxic responses of living organisms to xenobiotics exposure.MethodsIn order to investigate the influence of sex in inorganic mercury (Hg) exposure, male and female Wistar rats were exposed to 0.5, 1.0 and 1.5 mg Hg/kg body weight orally as HgCl2 twice a week for 12 weeks. ResultsWith the exception of the heart, higher Hg levels in the females as compared to males were observed in the animals. At the highest dose of inorganic Hg, female renal Hg content was 3.3 times higher than that of the males. Mixed sexual dimorphism characterised circulating-lipid- and organ-lipid lipotoxic and non-lipotoxic dyslipidemia. At the highest dose, hypercholesterolemia characterised the effect of inorganic Hg in the males as opposed to hypocholesterolemia in the female. Plasma and erythrocyte free fatty acids increased in both sexes, although the increase was more pronounced in the male. Reverse cholesterol transport was inhibited in the male at the highest dose of Hg as evidenced by decreased HDL cholesterol, whereas female HDL became enriched with cholesterol. Female erythrocytes had all their lipids increased by inorganic Hg, whereas only male erythrocyte triglyceride increased. Brain cholesterol and phospholipids, and splenic phospholipids were depleted in both sexes. Hepatic and pulmonary cholesterogenesis was induced in male animals, whereas cardiac cholesterogenesis and depletion of renal cholesterol were observed in the female. The male heart had its cholesterol depleted whereas renal cholesterol was not affectedConclusionOur findings indicate that inorganic Hg exposure appears to affect Hg and lipid kinetics differently in both sexes, thus underscoring the need to develop sex-tailored approaches in the treatment of metal toxicosis and its metabolic outcomes.
First detection of a plasmid-encoded New-Delhi metallo-beta-lactamase-1 (NDM-1) producing Acinetobacter baumannii using whole genome sequencing, isolated in a clinical setting in Benin
Authors: Yehouenou Carine Laurence , Bogaerts Bert , Vanneste Kevin , Roosens Nancy H.C. , Keersmaecker Sigrid C.J. De , Marchal Kathleen , Affolabi Dissou , Soleimani Reza , Rodriguez-Villalobos Hector , Bambeke Françoise Van , Dalleur Olivia , Simon Anne 
Published: September 2020

Abstract
Background: Carbapenem-resistant Acinetobacter baumannii is considered a top priority pathogen by the World Health Organization for combatting increasing antibiotic resistance and development of new drugs. Since it was originally reported in Klebsiella pneumoniae in 2009, the quick spread of the blaNDM-1 gene encoding a New-Delhi metallo-beta-lactamase-1 (NDM-1) is increasingly recognized as a serious threat. This gene is usually carried by large plasmids and has already been documented in diverse bacterial species, including A. baumannii. Here, we report the first detection of a NDM-1-producing A. baumannii strain isolated in Benin.Case presentation: A 31-year-old woman was admitted to a surgical unit with a diagnosis of post-cesarean hematoma. An extensively-drug resistant A. baumannii strain solely susceptible to amikacin, colistin and ciprofloxacin, and resistant to several other antibiotics including ceftazidime, imipenem, meropenem, gentamicin, tobramycin, ceftazidime/avibactam, and sulfamethoxazole-trimethoprim, was isolated from the wound. Production of NDM-1 was demonstrated by immunochromatographic testing. Whole genome sequencing of the isolate confirmed the presence of blaNDM-1, but also antibiotic resistance genes against multiple beta-lactamases and other classes of antibiotics, in addition to several virulence genes. Moreover, the blaNDM-1 gene was found to be present in a Tn125 transposon integrated on a plasmid.Conclusions: The discovery of this extensively-drug resistant A. baumannii strain carrying blaNDM-1 in Benin is worrying, especially because of its high potential risk of horizontal gene transfer due to being integrated in a transposon located on a plasmid. Strict control and prevention measures should be taken, once NDM-1 positive A. baumannii has been identified to prevent transfer of this resistance gene to other Enterobacterales. Capacity building is required by governmental agencies to provision suitable antibiotic treatment options and strategies, in combination with strengthening laboratory services for detection and surveillance of this pathogen.
Decrease of exercise endurance in critically ill COVID-19 survivors: 4 case reports
Authors: Geng Qian , Yu Xinxin , Shen Beilan , Li Shiyue , Zheng Jinping , Zhong Liping , Hong Yi , Huang Xiaoyan , Zeng Qingsi , Li Shaoqiang , Ye Feng , Guan Weijie , Xie Yanqing , Zhong Nanshan , Gao Yi 
Published: September 2020

Abstract
Background: The Coronavirus Disease 2019 (COVID-19) already have been as a pandemic. However, knowledge about the sequelae of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains limited. Here we descirbe the pulmonary function test (PFT) and cardiopulmonary exercise test (CPET) of critically ill COVID-19 in four cases with sereve acute respiratory distress syndrome (ARDS) after discharge.Case presentation: We introduce four patients who complained of fever, cough, chest tightness and other symptoms, all of them were confirmed as SARS-CoV-2 infection by real-time reverse transcription polymerase chain reaction (RT-PCR). They were treated with mechanical ventilation because of severe ARDS. After respiratory support, antiviral and anti-infective treatment, they were weaned from mechanic ventilation with the improvement of hypoxemia. All patients were discharged from the hospital after completion of treatment and had no mortality. Around 1-month post-discharge, they were followed up for chest computed tomography (CT) scan, and performed PFT and CPET. Peak oxygen uptake of predicted (peakVO2% pred) decreased in all four cases, although spirometry were in the normal range, and only 2 cases had mild decline in carbon monoxide diffusion capacity of predicted (DLCO%pred).Conclusions: We found reduced exercise endurance in all four COVID-19 survivors, even parts of them with normal or slightly abnormal static lung function. We also believe that exercise endurance impairment of COVID-19 convalescents is more likely affected by extrapulmonary factors. Taken the above into consideration, our study highlights that the combination of PFT and CPET are important tests for tracking the development and recovery of COVID-19 survivors.
Achievement of Long-term Local Control After Concurrently With Radiation and Anti-PD-1 Immunotherapy in Locally Advanced Non-small Cell Lung Cancer Patients
Authors: jing zhao , Zhou Rongjin , He Huaxiang , Wu Shixiu 
Published: September 2020

Abstract
Background: Although concurrent chemoradiotherapy (CRT) was recommend as standard of care in patients with stage III unresectable non-small cell lung cancer (NSCLC), many patients refused or were not eligible for chemotherapy in clinical practice. These patients often receive RT alone with 5-year OS rate of about 5-6%. This addressed a common clinical challenge of treating these patients. Immune-checkpoint inhibitors have demonstrated objective antitumor responses in patients with advanced NSCLC, but it is unclear how these agents can be used in the curative therapy with concurrent radiation. Case presentation: Here we described, the case series, the effect of stage III unresectable NSCLC patients who refused chemotherapy received radiation and anti-PD-1 immunotherapy. Three patients with stage III unresectable NSCLC were treated with radiotherapy concurrently with anti-PD-1 agent (pembrolizumab) between May 2018 and August 2018 in our hospital. Two patients experienced partial response and one patient experienced stable disease. One patient developed the liver metastasis 4 months after the treatment. All patients had no local-regional recurrence. No patient experienced ≥ grade 3 adverse event (AE), and no patient discontinued treatment because of an AE. Conclusions: Concurrent treatment with radiation and pembrolizumab for unresectable stage III NSCLC patients who refused chemotherapy demonstrated its efficacy and acceptable tolerance. Further investigations are warranted to determine its role in the management of these patients.
Prognostic and Clinicopathological Significance of CXCL1 Expression in Gastric&nbsp;Cancer: A Meta-Analysis.
Authors: Xia Yujian , Wei ZheWei , Huang WeiBin , Wei XiaoJing , He YuLong 
Published: September 2020

Abstract
Background: Some studies have shown that CXCL1 expression in gastric cancer (GC) is associated with survival and clinicopathological characteristics. However, the evidence remains inconclusive. Thus, we aim to further explore the clinicopathological significance and potential prognostic role of CXCL1 expression in GC.Methods: Databases of EMBASE, PubMed Web of Science and the Cochrane Library were systematically searched for the eligible studies from their establishment to July 16, 2020, which reported the association between CXCL1 expression and survival in GC. The quantitative meta-analysis was carried out with Stata SE12.0 software. Results: A total of 1474 patients from 9 eligible studies were included in the present meta-analysis. Our results demonstrated that elevated expression level of CXCL1 was significantly associated with poor overall survival (OS) (HR=1.68; 95%CI: 1.26-2.24, P<0.001). The subgroup analysis revealed that elevated CXCL1 expression was found to be associated with a poor OS in Chinese and Japanese patients. Moreover, the stratification analysis by detection methods showed that elevated CXCL1 expression had a significantly poor OS effect on GC patients by IHC but not by RT-PCR. Besides, high CXCL1 expression was obviously associated with higher depth of tumor invasion, earlier lymph node metastasis and more advanced TNM stage compared with low CXCL1 expression in GC.Conclusions: CXCL1 may serve as a potential biomarker to predict prognosis and clinicopathological features in GC.
Developmental Regulation of Dirofilaria Immitis Microfilariae and Evaluation of Ecdysone Signaling Pathway Transcript Level Using Droplet Digital Polymerase Chain Reaction
Authors: Kuan Tsai-Chi Shang , Prichard Roger K 
Published: September 2020

Abstract
BackgroundCurrent Dirofilaria immitis prevention relies on macrocyclic lactones but evidence of drug-resistant isolates has called for the need for alternative approaches to disease intervention. As microfilariae are known to be in a state of developmental arrest in their mammalian host and then undergo a rapid molt once inside the arthropod, this study aimed to look at the developmental regulation of D. immitis microfilariae that occurs in their arthropod host using in vitro approaches and to investigate the role of the ecdysone signaling system in this development.MethodsD. immitis microfilariae extracted from dog blood were incubated in various media conditions to identify suitable conditions for in vitro culture and development, and to determine the effects of FBS, mosquito cells, and ecdysteroid on the development of the microfilariae. Transcript level of the ecdysone signaling pathway components were measured with droplet digital PCR.ResultsIn vitro conditions that best promote early development of D. immitis microfilariae to the “late sausage stage” have been identified. FBS showed inhibitory effects on the development and motility of the microfilariae, but media conditioned with Anopheles gambiae cells was favorable to microfilarial growth. Transcript level study using droplet digital PCR also showed that developing microfilariae displayed upregulation of the ecdysone signaling system components, and 20‑hydroxyecdysone increased the proportion of larvae developing to the sausage and late sausage stages in vitro.ConclusionsThe arthropod host environment provides cues required for the rapid development of the D. immitis microfilariae and the ecdysone signaling system may play an important role in filarial nematode developmental transitions. This study contributes to a better understanding the developmental process of D. immitis microfilariae.
Terminal Groups Dependent Near-Field Enhancement Effect of Ti3C2Tx Nanosheets
Authors: Yang Ying-Ying , Zhou Wen-Tao , Song Wei-Long , Zhu Qing-Quan , Xiong Hao-Jiang , Zhang Jue , Cheng Sheng , Luo Pai-Feng , Lu Ying-Wei 
Published: September 2020

Abstract
Both multilayered (ML) and few-layered (FL) Ti3C2Tx nanosheets with different dominant terminal groups have been prepared through a typical etching and delaminating procedure. Various characterizations confirm that the physical and chemical performance of the nanosheets are dependent on the dominant functional groups. It has been demonstrated that ML-Ti3C2Tx has been mainly terminated by O-related groups, which result in better oxidation resistance and stronger near-field enhancement effect. As for FL-Ti3C2Tx, which is mainly terminated by hydroxyl groups, it can be better dispersed in aqueous solution and could confine stronger near-field after coupling to Ag nanostructures by electron injection.
Characteristic and Potential Therapeutic Effect of Isolated MDR-Acinetobacter Baumannii Lytic Phage
Authors: Sisakhtpour Behnam , Mirzaei Arezoo , Karbasizadeh Vajihe , Hosseini Nafise Sadat , Shabani Mehdi , Moghim Sharareh 
Published: September 2020

Abstract
Background: Acinetobacter baumannii is a major pathogen in the hospital, especially in Intensive Care Units (ICU) and the resistance to multiple drugs as a major contributor to hospital infection. Bacteriophages are viruses that attack bacteria and kill them that could be used for clinical treatment. The aim of the study is in evaluating the function of bacteriophage specificity of multi-drug resistant Acinetobacter baumannii, to be used as a useful method for treating of Acinetobacter Infections.Methods: Cross-sectional study during the year 2017, from patients admitted to the ICU, First, 48 isolates of Acinetobacter baumannii were identified by phenotypic method and amplified with blaOXA-51 gene. Then, the sensitivity of phages to pathogens namely ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp) evaluated. DNA of the phage was extracted using the Viral Nucleic Extraction Kit II (Geneaid, Taipei, Taiwan) according to the manufacturer's instructions. Then for protein analysis, PEG-precipitated purified phages were subjected directly to SDS-PAGE, and protein bands were visualized by coomassie Blue G-250 staining method. Finally for cell survival assay we investigated the toxicity of the isolated phage to Hela cells.Results: In the bacterial resistance pattern, the highest resistance belongs to ciprofloxacin. In optimal phage test, at dilution of 1 (MOI 1) it produced the best effect on bacteria in 30 minutes. Phage sensitivity to different hosts performed by double layer agar method, the phage was treated with ESKAPE bacteria and after 24 hours’ incubation at 37°C, only for Acinetobacter baumannii Plaque created. The genome analysis indicated that phage pIsf-AB2 has a double-stranded DNA genome. In bacterial control, all cells were killed by A. baumannii, and no live-cell was seen. The cells remained in control of the phage, and the phage did not affect the cells.Conclusion: Our findings support the potential application of the phage with potent endolysin activity against MDR A. baumannii and give useful information for its further study and use.