bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metal Coated Polypropylene Separator with Enhanced Surface Wettability for High Capacity Lithium Metal Batteries
Authors: Din Mir Mehraj Ud , Murugan Ramaswamy 
Published: November 2019

AbstractLithium metal batteries are among the strong contenders to meet the increasing energy demands of the modern world. Metallic lithium (Li) is light in weight, possesses very low standard negative electrochemical potential and offers an enhanced theoretical capacity (3860 mA h g−1). As a negative electrode Li paves way to explore variety of elements including oxygen, sulfur and various other complex oxides as potential positive electrodes with a promise of much higher energy densities than that of conventional positive electrodes. However, there are technical challenges in utilizing metallic lithium due to its higher reactivity towards liquid electrolytes and higher affinity to form Li dendrites, leading to serious safety concerns. Here, we report on preparation of niobium (Nb) metal-coated binder-free and highly hydrophilic polypropylene separator prepared via radio frequency (RF) magnetron sputtering. Thin layer of niobium metal (Nb) particles were deposited onto the polypropylene (PP) sheet for various time periods to achieve desired coating thickness. The as-prepared separator revealed excellent hydrophilic behaviour due to enhanced surface wettability. Symmetric cells display reduced interface resistance and uniform voltage profiles for 1000 cycles with reduced polarization at higher current densities suggesting smooth stripping and plating of Li and homogeneous current distribution at electrode/electrolyte interface under room temperature conditions. Nb nanolayer protected separator with LiNi0.33M0.33Co0.33O2 (LNMC) and composite sulfur cathodes revealed an enhanced cycling stability.
Identification of candidate molecular targets of the novel antineoplastic antimitotic NP-10
Authors: Yokoyama Takuya , Yukuhiro Masaki , Iwasaki Yuka , Tanaka Chika , Sankoda Kazunari , Fujiwara Risa , Shibuta Atsushi , Higashi Taishi , Motoyama Keiichi , Arima Hidetoshi , Yoshida Kazumasa , Sugimoto Nozomi , Morimoto Hiroyuki , Kosako Hidetaka , Ohshima Takashi , Fujita Masatoshi 
Published: November 2019

AbstractWe previously reported the identification of a novel antimitotic agent with carbazole and benzohydrazide structures: N′-[(9-ethyl-9H-carbazol-3-yl)methylene]-2-iodobenzohydrazide (code number NP-10). However, the mechanism(s) underlying the cancer cell-selective inhibition of mitotic progression by NP-10 remains unclear. Here, we identified NP-10-interacting proteins by affinity purification from HeLa cell lysates using NP-10-immobilized beads followed by mass spectrometry. The results showed that several mitosis-associated factors specifically bind to active NP-10, but not to an inactive NP-10 derivative. Among them, NUP155 and importin β may be involved in NP-10-mediated mitotic arrest. Because NP-10 did not show antitumor activity in vivo in a previous study, we synthesized 19 NP-10 derivatives to identify more effective NP-10-related compounds. HMI83-2, an NP-10-related compound with a Cl moiety, inhibited HCT116 cell tumor formation in nude mice without significant loss of body weight, suggesting that HMI83-2 is a promising lead compound for the development of novel antimitotic agents.
Segregation of age-related skin microbiome characteristics by functionality
Authors: Kim Hye-Jin , Kim Jin Ju , Myeong Nu Ri , Kim Taeyune , Kim DooA , An Susun , Kim Hanbyul , Park Taehun , Jang Sue Im , Yeon Jae Ho , Kwack Ilyoung , Sul Woo Jun 
Published: November 2019

AbstractAlthough physiological changes are the most evident indicators of skin aging by alteration of the skin’s structure and function, we question whether skin aging is also affected by the structure and assembly process of the skin microbiome. We analysed the skin microbiomes of 73 healthy Chinese women in two age groups (25–35 years old and 56–63 years old) using 16S rRNA gene amplicon sequencing; the overall microbiome structure was significantly different between the two age groups. An analysis using ecological theory to evaluate the process of microbial community assembly processes revealed that the microbiomes of the older group were formed under a greater influence of the niche-based process, with the network of microbes being more collapsed than that of the younger group. Inferred metagenomic functional pathways associated with replication and repair were relatively more predominant in the younger group whereas, among the various metabolism-related pathways, those associated with biodegradation were more predominant in the older group. Interestingly, we found two segregated sub-typing patterns in the younger group which were also observed in the skin microbiomes of young Chinese women living in four other cities in China. The results of our study highlights candidate microbes and functional pathways that are important for future research into preventing skin aging and which could lead to a comprehensive understanding of age-related skin microbiome characteristics.
The VP3 protein of duck hepatitis A virus mediates host cell adsorption and apoptosis
Authors: Lai Yalan , Zeng Ni , Wang Mingshu , Cheng Anchun , Yang Qiao , Wu Ying , Jia Renyong , Zhu Dekang , Zhao XinXin , Chen Shun , Liu Mafeng , Zhang Shaqiu , Wang Yin , Xu Zhiwen , Chen Zhengli , zhu Ling , Luo Qihui , Liu Yunya , Yu Yanling , Zhang Ling , Huang Juan , Tian Bin , Pan Leichang , Ur Rehman Mujeeb , Chen Xiaoyue 
Published: November 2019

AbstractDuck hepatitis A virus (DHAV) causes an infectious disease that mainly affects 1- to 4-week-old ducklings, resulting in considerable loss to the duck industry. Although there have been many studies on DHAV in recent years, the effects on host infection and pathogenesis of DHAV-1 remain largely unknown. This study investigated the effects of the DHAV-1 structural protein VP3 on DHAV-1 virus adsorption and apoptosis to explore the role of VP3 in the viral life cycle. The effects of DHAV-1 VP3 and an antibody against the protein on virion adsorption was analyzed by qRT-PCR. The results showed that the virus copy number for the rabbit anti-VP3 IgG-treated group was significantly lower than that for the negative control group but higher than that for the rabbit anti-DHAV-1 IgG-treated group. This result indicates that VP3 mediates DHAV-1 virus adsorption but that it is not the only protein that involved in this process. In addition, a eukaryotic recombinant plasmid, pCAGGS/VP3, was transfected into duck embryo fibroblasts (DEFs), and the apoptotic rate was determined by DAPI staining, the TUNEL assay and flow cytometry. DAPI staining showed nucleus fragmentation and nuclear edge shifting. TUNEL assay results revealed yellow nuclei, and flow cytometry indicated a significant increase in the apoptotic rate. In addition, qRT-PCR revealed increased in the transcriptional levels of the apoptotic caspase-3, −8 and −9, with the largest increase for caspase-3, followed by caspase-9 and caspase-8. Enzyme activity analysis confirmed these results. Furthermore, the VP3 protein decreased the mitochondrial membrane potential, and the transcriptional levels of the proapoptotic factors Bak, Cyt c and Apaf-1 in the mitochondrial apoptotic pathway were significantly upregulated. These data suggest that expression of VP3 in DEFs induces apoptosis and may primarily activate caspase-3-induced apoptosis through mitochondrion-mediated intrinsic pathways. The findings provide scientific data to clarify DHAV-1 infection and pathogenesis.
Postoperative serum metabolites of patients on a low carbohydrate ketogenic diet after pancreatectomy for pancreatobiliary cancer: a nontargeted metabolomics pilot study
Authors: Kang Chang Moo , Yun BoKyeong , Kim Minju , Song Mina , Kim Yeon-hee , Lee Sung Hwan , Lee Hosun , Lee Song Mi , Lee Seung-Min 
Published: November 2019

AbstractA ketogenic diet is a potential adjuvant cancer therapy that limits glucose availability to tumours while fuelling normal tissues with ketone bodies. We examined the effect of a low carbohydrate ketogenic diet (LCKD) (80% kcal from fat, ketogenic ratio 1.75:1, w/w) compared to a general hospital diet (GD) on serum metabolic profiles in patients (n = 18, ≥ 19 years old) who underwent pancreatectomy for pancreatobiliary cancer. Serum samples collected preoperatively (week 0) and after the dietary intervention (week 2) were analysed with a nontargeted metabolomics approach using liquid chromatography–tandem mass spectrometry. Serum β-hydroxybutyrate and total ketone levels significantly increased after 2 weeks of LCKD compared to GD (p < 0.05). Principal component analysis score plots and orthogonal partial least squares discriminant analysis also showed significant differences between groups at week 2, with strong validation. In all, 240 metabolites differed between LCKD and GD. Pathways including glycerophospholipid and sphingolipid metabolisms were significantly enriched in the LCKD samples. LCKD decreased C22:1-ceramide levels, which are reported to be high in pancreatic cancer, while increasing lysophosphatidylcholine (18:2), uric acid, citrulline, and inosine levels, which are generally low in pancreatic cancer. Postoperative LCKD might beneficially modulate pancreatic cancer-related metabolites in patients with pancreatobiliary cancer.
Distinctive patterns of peripheral neuropathy across the spectrum of plasma cell disorders
Authors: Byun Ja Min , Kwon Young Nam , Koh Youngil , Yoon Sung-Soo , Sung Jung-Joon , Kim Inho 
Published: November 2019

AbstractMany patients with plasma cell disorders suffer from peripheral neuropathy, but differential diagnosis with chronic inflammatory demyelinating polyneuropathy (CIDP) is difficult. We aimed to (1) identify factors useful for differential diagnosis between peripheral neuropathy associated with plasma cell disorders versus CIDP and (2) determine whether neuropathy presentations and severity varied across the spectrum of different plasma cell disorders. A retrospective chart review of 18 monoclonal gammopathy of unknown significance (MGUS) patients, 15 POEMS syndrome patients and 34 CIDP patients between January 2005 and December 2016 was conducted. The peripheral neuropathy associated with plasma cell disorders seemed to be more sensory oriented compared to CIDP. MGUS patients were significantly older than CIDP patients (median age 70 vs. 59, respectively, p = 0.027). POEMS syndrome patients showed significantly higher platelet count at the time of neuropathy presentation compared to CIDP (p = 0.028). Lambda type MGUS patients were associated with less severe symptoms compared to POEMS syndrome patients despite harboring lambda monoclonal gammopathy as a common denominator. Kappa type MGUS patients showed predominantly axonal type neuropathy compared to its counterpart and POEMS syndrome. Careful inspection of clinical profiles and symptoms of patients presenting with neuropathy can help to discriminate those with underlying plasma cell disorders. The phenotype of neuropathy, platelet count and age at presentation seem to be the most useful indicators.
A pilot study of peripheral blood DNA methylation models as predictors of knee osteoarthritis radiographic progression: data from the Osteoarthritis Initiative (OAI)
Authors: M. Dunn Christopher , Nevitt Michael C. , Lynch John A. , Jeffries Matlock A. 
Published: November 2019

AbstractKnee osteoarthritis (OA) is a leading cause of chronic disability worldwide, but no diagnostic or prognostic biomarkers are available. Increasing evidence supports epigenetic dysregulation as a contributor to OA pathogenesis. In this pilot study, we investigated epigenetic patterns in peripheral blood mononuclear cells (PBMCs) as models to predict future radiographic progression in OA patients enrolled in the longitudinal Osteoarthritis Initiative (OAI) study. PBMC DNA was analyzed from baseline OAI visits in 58 future radiographic progressors (joint space narrowing at 24 months, sustained at 48 months) compared to 58 non-progressors. DNA methylation was quantified via Illumina microarrays and beta- and M-values were used to generate linear classification models. Data were randomly split into a 60% development and 40% validation subsets, models developed and tested, and cross-validated in a total of 40 cycles. M-value based models outperformed beta-value based models (ROC-AUC 0.81 ± 0.01 vs. 0.73 ± 0.02, mean ± SEM, comparison p = 0.002), with a mean classification accuracy of 73 ± 1% (mean ± SEM) for M- and 69 ± 1% for beta-based models. Adjusting for covariates did not significantly alter model performance. Our findings suggest that PBMC DNA methylation-based models may be useful as biomarkers of OA progression and warrant additional evaluation in larger patient cohorts.
Crustal movement and strain distribution in East Asia revealed by GPS observations
Authors: Hao Ming , Li Yuhang , Zhuang Wenquan 
Published: November 2019

AbstractEast Asia is bounded by the Indian plate to the southwest and the Pacific and Philippine plates to the east, and has undergone complex tectonic evolution since ~55 Ma. In this study, we collect and process three sources of GPS datasets, including GPS observations, GPS positioning time series, and published GPS velocities, to derive unified velocity and strain rate fields for East Asia. We observed southward movement and arc-parallel extension along the Ryukyu Arc and propose that the maximum principal stress axis (striking NEE) in North China could be mainly induced by westward subduction of the Pacific plate and the southward movement of the Ryukyu Arc. The large EW-trending sinistral shear zone that bounds North China has been created by eastward movement of South China to the south and westward subduction of the Pacific plate to the north. GPS velocity profiles and strain rates also demonstrate that crustal deformation in mainland China is controlled by northeastward collision of the Indian plate into Eurasia and westward subduction of the Pacific and Philippine Sea plates beneath Eurasia. In particular, the India-Eurasia continental collision has the most extensive impact, which can reach as far as the southern Lake Baikal. The viscous behavior of the subducting Pacific slab also drives interseismic deformation of North China. The crustal deformation caused by Philippine oceanic subduction is small and is limited to the region between the southeast coast of mainland China and Taiwan island. However, the principal compressional strain around eastern Taiwan is the largest in the region.
A standalone bioreactor system to deliver compressive load under perfusion flow to hBMSC-seeded 3D chitosan-graphene templates
Authors: Lovecchio Joseph , Gargiulo Paolo , Vargas Luna Jose Luis , Giordano Emanuele , Sigurjónsson Ólafur Eysteinn 
Published: November 2019

AbstractThe availability of engineered biological tissues holds great potential for both clinical applications and basic research in a life science laboratory. A prototype standalone perfusion/compression bioreactor system was proposed to address the osteogenic commitment of stem cells seeded onboard of 3D chitosan-graphene (CHT/G) templates. Testing involved the coordinated administration of a 1 mL/min medium flow rate together with dynamic compression (1% strain at 1 Hz; applied twice daily for 30 min) for one week. When compared to traditional static culture conditions, the application of perfusion and compression stimuli to human bone marrow stem cells using the 3D CHT/G template scaffold induced a sizable effect. After using the dynamic culture protocol, there was evidence of a larger number of viable cells within the inner core of the scaffold and of enhanced extracellular matrix mineralization. These observations show that our novel device would be suitable for addressing and investigating the osteogenic phenotype commitment of stem cells, for both potential clinical applications and basic research.
Altered fluvial patterns in North China indicate rapid climate change linked to the Permian-Triassic mass extinction
Authors: Zhu Zhicai , Liu Yongqing , Kuang Hongwei , Benton Michael J. , Newell Andrew J. , Xu Huan , An Wei , Ji Shu’an , Xu Shichao , Peng Nan , Zhai Qingguo 
Published: November 2019

AbstractThe causes of the severest crisis in the history of life around the Permian-Triassic boundary (PTB) remain controversial. Here we report that the latest Permian alluvial plains in Shanxi, North China, went through a rapid transition from meandering rivers to braided rivers and aeolian systems. Soil carbonate carbon isotope (δ13C), oxygen isotope (δ18O), and geochemical signatures of weathering intensity reveal a consistent pattern of deteriorating environments (cool, arid, and anoxic conditions) and climate fluctuations across the PTB. The synchronous ecological collapse is confirmed by a dramatic reduction or disappearance of dominant plants, tetrapods and invertebrates and a bloom of microbially-induced sedimentary structures. A similar rapid switch in fluvial style is seen worldwide (e.g. Karoo Basin, Russia, Australia) in terrestrial boundary sequences, all of which may be considered against a background of global marine regression. The synchronous global expansion of alluvial fans and high-energy braided streams is a response to abrupt climate change associated with aridity, hypoxia, acid rain, and mass wasting. Where neighbouring uplands were not uplifting or basins subsiding, alluvial fans are absent, but in these areas the climate change is evidenced by the disruption of pedogenesis.
Non-Gaussian models of diffusion weighted imaging for detection and characterization of prostate cancer: a systematic review and meta-analysis
Authors: Brancato V. , Cavaliere C. , Salvatore M. , Monti S. 
Published: November 2019

AbstractThe importance of Diffusion Weighted Imaging (DWI) in prostate cancer (PCa) diagnosis have been widely handled in literature. In the last decade, due to the mono-exponential model limitations, several studies investigated non-Gaussian DWI models and their utility in PCa diagnosis. Since their results were often inconsistent and conflicting, we performed a systematic review of studies from 2012 examining the most commonly used Non-Gaussian DWI models for PCa detection and characterization. A meta-analysis was conducted to assess the ability of each Non-Gaussian model to detect PCa lesions and distinguish between low and intermediate/high grade lesions. Weighted mean differences and 95% confidence intervals were calculated and the heterogeneity was estimated using the I2 statistic. 29 studies were selected for the systematic review, whose results showed inconsistence and an unclear idea about the actual usefulness and the added value of the Non-Gaussian model parameters. 12 studies were considered in the meta-analyses, which showed statistical significance for several non-Gaussian parameters for PCa detection, and to a lesser extent for PCa characterization. Our findings showed that Non-Gaussian model parameters may potentially play a role in the detection and characterization of PCa but further studies are required to identify a standardized DWI acquisition protocol for PCa diagnosis.
‘Normal’ hearing thresholds and fundamental auditory grouping processes predict difficulties with speech-in-noise perception
Authors: Holmes Emma , Griffiths Timothy D. 
Published: November 2019

AbstractUnderstanding speech when background noise is present is a critical everyday task that varies widely among people. A key challenge is to understand why some people struggle with speech-in-noise perception, despite having clinically normal hearing. Here, we developed new figure-ground tests that require participants to extract a coherent tone pattern from a stochastic background of tones. These tests dissociated variability in speech-in-noise perception related to mechanisms for detecting static (same-frequency) patterns and those for tracking patterns that change frequency over time. In addition, elevated hearing thresholds that are widely considered to be ‘normal’ explained significant variance in speech-in-noise perception, independent of figure-ground perception. Overall, our results demonstrate that successful speech-in-noise perception is related to audiometric thresholds, fundamental grouping of static acoustic patterns, and tracking of acoustic sources that change in frequency. Crucially, speech-in-noise deficits are better assessed by measuring central (grouping) processes alongside audiometric thresholds.
Possible indication of endoscopic resection in undifferentiated early gastric cancer
Authors: Ryu Dae Gon , Choi Cheol Woong , Kim Su Jin , Kang Dae Hwan , Kim Hyung Wook , Park Su Bum , Nam Hyeong Seok 
Published: November 2019

AbstractEndoscopic resection for early gastric cancer (EGC) without lymph node metastasis may be a valuable treatment option. To date, endoscopic resection for undifferentiated EGC is being investigated. We evaluated the risk of lymph node metastasis in undifferentiated EGC by examining the preoperative endoscopic findings and operated pathologic specimen. The medical records of patients who underwent surgical resection because of undifferentiated EGC between November 2008 and December 2015 were reviewed retrospectively. The risk factors associated with lymph node metastasis and the lymph node metastasis rate in the expanded indication of undifferentiated EGC were evaluated. A total of 376 patients with undifferentiated EGC (233 signet ring cell type and 143 poorly differentiated type) were analyzed. Lymph node metastasis was found in 9.8% of the patients. Among the patients who met the expanded criteria (59 patients), only one patient had lymph node metastasis (signet ring cell type without ulceration and 15 mm in size). The risk factors associated with lymph node metastasis were lesion size >20 mm (OR 3.013), scar deformity (OR 2.248), surface depression (OR 2.360), submucosal invasion (OR 3.427), and lymphovascular invasion (OR 6.296). Before endoscopic resection of undifferentiated EGC, careful selection of patients should be considered. The undifferentiated EGC with size ≥15 mm, scar deformity, surface depression, submucosal invasion, and lymphovascular invasion should be considered surgical resection instead of endoscopic resection.