bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunohistochemical assessment of HRAS Q61R mutations in breast adenomyoepitheliomas
Authors: Pareja Fresia , Toss Michael S , Geyer Felipe C , da Silva Edaise M , Vahdatinia Mahsa , Sebastiao Ana Paula M , Selenica Pier , Szatrowski Austin , Edelweiss Marcia , Wen Hannah Y , Mihai Raluca , Varga Zsuzsanna , Foschini Maria P , Rubin Brian P , Ellis Ian O , Chandarlapaty Sarat , Jungbluth Achim A , Brogi Edi , Weigelt Britta , Reis‐Filho Jorge S , Rakha Emad A 
Published: December 2019

AimsBreast adenomyoepitheliomas (AMEs) are uncommon tumours. Most oestrogen receptor (ER)‐positive AMEs have mutations in phosphoinositide 3‐kinase (PI3K) pathway genes, whereas ER‐negative AMEs usually harbour concurrent mutations affecting the HRAS Q61 hotspot and PI3K pathway genes. Here, we sought to determine the sensitivity and specificity of RAS Q61R immunohistochemical (IHC) analysis for detection of HRAS Q61R mutations in AMEs.Methods and resultsTwenty‐six AMEs (14 ER‐positive; 12 ER‐negative) previously subjected to massively parallel sequencing (n = 21) or Sanger sequencing (n = 5) of the HRAS Q61 hotspot locus were included in this study. All AMEs were subjected to IHC analysis with a monoclonal (SP174) RAS Q61R‐specific antibody, in addition to detailed histopathological analysis. Nine ER‐negative AMEs harboured HRAS mutations, including Q61R (n = 7) and Q61K (n = 2) mutations. Five of seven (71%) AMEs with HRAS Q61R mutations were immunohistochemically positive, whereas none of the AMEs lacking HRAS Q61R mutations (n = 17) were immunoreactive. RAS Q61R immunoreactivity was restricted to the myoepithelium in 80% (4/5) of cases, whereas one case showed immunoreactivity in both the epithelial component and the myoepithelial component. RAS Q61R immunohistochemically positive AMEs were associated with infiltrative borders (P < 0.001), necrosis (P < 0.01) and mitotic index in the epithelial (P < 0.05) and myoepithelial (P < 0.01) components. RAS Q61R IHC assessment did not reveal Q61K mutations (0/2).ConclusionsIHC analysis of RAS Q61R shows high specificity (100%) and moderate sensitivity (71%) for detection of HRAS Q61R mutations in breast AMEs, and appears not to detect HRAS Q61K mutations. IHC analysis of RAS Q61R may constitute a useful technique in the diagnostic workup of ER‐negative AMEs.
How helpful are the European AIDS Clinical Society cognitive screening questions in predicting cognitive impairment in an aging, well‐treated HIV‐positive population?
Authors: Metral M , Nadin I , Locatelli I , Tarr PE , Calmy A , Kovari H , Brugger P , Cusini A , Gutbrod K , Schmid P , Schwind M , Kunze U , Di Benedetto C , Pignatti R , Du Pasquier R , Darling KEA , Cavassini M ,   the Neurocognitive Assessment in the Metabolic and Aging Cohort (NAMACO) study group, Swiss HIV Cohort Study
Published: December 2019

ObjectivesDiagnosing neurocognitive impairment (NCI) in HIV infection requires time‐consuming neuropsychological assessment. Screening tools are needed to identify when neuropsychological referral is indicated. We examined the positive and negative predictive values (PPVs and NPVs, respectively) of the three European AIDS Clinical Society (EACS) screening questions in identifying NCI.MethodsThe Neurocognitive Assessment in the Metabolic and Aging Cohort (NAMACO) study recruited patients aged ≥45 years enrolled in the Swiss HIV Cohort Study between 1 May 2013 and 30 November 2016. NAMACO participants (1) answered EACS screening questions, (2) underwent standardized neuropsychological assessment and (3) completed self‐report forms [Center for Epidemiologic Studies Depression Scale (CES‐D)] rating mood. NCI categories were defined using Frascati criteria. PPVs and NPVs of the EACS screening questions in identifying NCI categories were calculated.ResultsOf 974 NAMACO participants with complete EACS screening question data, 244 (25.1%) expressed cognitive complaints in answer to at least one EACS screening question, of whom 51.3% had NCI (26.1% HIV‐associated and 25.2% related to confounding factors). The PPV and NPV of the EACS screening questions in identifying HIV‐associated NCI were 0.35 and 0.7, respectively. Restricting analysis to NCI with functional impairment or related to confounding factors, notably depression, the NPV was 0.90. Expressing cognitive complaints for all three EACS screening questions was significantly associated with depression (P < 0.001).ConclusionsThe EACS screening questions had an NPV of 0.7 for excluding patients with HIV‐associated NCI as defined by Frascati criteria. The PPV and NPV may improve if NCI diagnoses are based on new criteria.
Preparation, characterization, and physical properties of lightweight ceramics by using sewage sludge ash
Authors: Negm Asmaa A. , Elrasasi Tarek Y. , Khoder Hanem A. , Metawe Fatma M. , El‐Mansy Mabrouk K. 
Published: December 2019

AbstractIn the present study, sewage sludge ash (SSA) was added to clay to prepare lightweight ceramics for sustainable construction materials. The characterization and the effect of different concentrations of SSA on the physical and mechanical properties of the samples were studied. The results showed that the organic matter in SSA facilitated the combustion process. SSA addition reduced the bulk density from (1.94 to 1.32 g/cm3). Otherwise, the water absorption, the apparent porosity and the loss on ignition increased with the increase in SSA concentration. The addition of SSA lowered the compression strength but still within the standard range of the construction materials at concentration up to (30 wt.%). Furthermore, heavy metals are solidified inside the sintered samples, since Cu, Cd, Fe, Zn, Cr, Mn, Ni, and Pb, concentrations in the leachate met the range of Egyptian standard specification.
Investigation on corrosion and wear resistance of MgO‐Al2O3 composite coating prepared by plasma electrolytic oxidation
Authors: Liu Xin‐tong , Wang Dong‐dong , Wu Ye‐kang , Yang Zhong , Li Dalong , Shen Dejiu 
Published: December 2019

AbstractCeramic coatings were prepared on 6061 Al alloy in a mixed electrolyte with/without MgO powders at different treatment durations. The results of energy dispersive spectroscopy (EDS) and X‐ray diffraction (XRD) showed that MgO powder was incorporated into the coatings, and Mg species gradually aggregated into coating inside as prolonging the oxidation time. Scanning electron microscopy (SEM) showed that MgO additive had a certain effect on the microstructures and coating thickness. The corrosion behavior tests evaluated by potentiodynamic polarization tests and electrochemical impedance spectroscopy (EIS) in 3.5 wt% NaCl solution suggested that at the same treatment time, the addition of MgO powders can improve the corrosion resistance of the coating, and the Mg‐rich layer can affect the corrosion resistance of the coating. The tests of mechanical properties showed that the addition of MgO powders improved the stability and hardness of the coating.
Permeability behavior of silicon carbide‐based membrane and performance study for oily wastewater treatment
Authors: Das Dulal , Kayal Nijhuma , Marsola Gabriel Antonio , Damasceno Leonardo Augusto , Innocentini Murilo Daniel de Mello 
Published: December 2019

AbstractPorous SiC ceramic is considered as a suitable material for hot gas filtration, microfiltration, and many others industrial applications. However, full utilizations of porous SiC ceramics have been limited by high‐processing costs. In this study, mullite‐bonded porous SiC ceramics membranes were prepared using commercial SiC powder, alumina, clay, and different sacrificial pore formers. The effect of different pore formers on the microstructure, mechanical strength, porosity and pore size distribution, air, and water permeability of porous SiC ceramics were investigated. The average pore diameter, porosities, and flexural strength of the final ceramics varied in the range 3.7‐6.5 µm, 38‐50 vol. %, and 28‐38 MPa, respectively, depending on the characteristics of pore former. The Darcian (k1) and non‐Darcian (k2) permeability evaluated from air permeation behavior at room temperature was found to vary from 1.48 × 10−13 to 4.64 × 10−13 m2 and 1.46 × 10−8 to 6.51 × 10−8 m, respectively. All membranes showed high oil rejection rate (89%‐93%) from feed wastewater with oil concentration of 1557 mg/L. The membrane with porosity ~48 vol% and mechanical strength 31.5 MPa showed and highest pure water permeability of 13 298 Lm−2h−1bar−1.
Petits‐enfants de Keynes, tâcherons de Marx: pourquoi le travail humain conserve toute son importance
Authors: EKBIA Hamid R. , NARDI Bonnie A. 
Published: December 2019

RésuméL'inquiétude que suscitent partout dans le monde l'automatisation et son influence sur l'«avenir du travail» découle de certaines idées répandues sur la technologie, l'activité humaine et la valeur économique. Le débat n'est pas nouveau et intéressait déjà des penseurs éminents comme Marx et Keynes. Pour mieux appréhender la situation actuelle, les auteurs réexaminent la question à la lumière de l'histoire du capitalisme. En mettant l'accent sur la place du travail et sur la technologie, ils décrivent des formes cachées de création de valeur dans l'économie contemporaine et abordent certains aspects encore inédits dans le débat historique, avant de présenter plusieurs scénarios possibles pour l'avenir.
Progrès économiques et avancées sociales au sein des chaînes d'approvisionnement mondiales: plusieurs exemples dans les pays du cône Sud
Authors: REINECKE Gerhard , POSTHUMA Anne 
Published: December 2019

RésuméLes auteurs s'interrogent sur la nature et la diffusion des progrès induits par les chaînes d'approvisionnement mondiales (CAM), en s'appuyant sur les conclusions de neuf études de cas portant sur des filières implantées dans des pays du sud de l'Amérique latine. Des progrès économiques, associés à certaines avancées en matière sociale, sont observés parmi les entreprises têtes de file, mais les fournisseurs en amont n'en tirent pas toujours profit. Après avoir isolé trois types d'évolutions possibles au sein des CAM (développements «antagoniste», «tronqué» et «intégré»), les auteurs formulent des recommandations en ce qui concerne l'action des pouvoirs publics, des entreprises et des acteurs sociaux.
Introduction – Travail, emploi, société: que nous réserve l'avenir?
Authors: Rani Uma , Grimshaw Damian 
Published: December 2019

RésuméPartant de la matière de ce numéro spécial, les auteurs mettent en évidence les grands thèmes de recherche sur l'avenir du travail. Ils décrivent les mutations accélérées du monde du travail qui offrent des possibilités de croissance génératrice d'égalité, mais avec le risque de mal gérer le changement technique, les inégalités (en insistant sur le caractère intersectionnel et persistant des inégalités entre hommes et femmes), les chaînes d'approvisionnement mondiales et les nouvelles possibilités de dialogue social. Partant, ils formulent des recommandations pour renforcer la protection des travailleurs et les institutions qui les représentent, revoir les cadres réglementaires et les systèmes fiscaux, et enfin assurer des transitions équitables.
Introducción. Trabajo, empleo y sociedad: ¿Qué esperar del futuro?
Authors: RANI Uma , GRIMSHAW Damian 
Published: December 2019

ResumenEn la presente introducción se destacan los principales aspectos extraídos de los artículos de este número especial que cabría considerar en las investigaciones sobre el futuro del trabajo. Se presentan los cambios acelerados que afectan al mundo del trabajo como una oportunidad de orientarse hacia un crecimiento proclive a la igualdad, pero también se advierte de los peligros que plantea una mala gestión del cambio tecnológico, de las desigualdades –haciendo hincapié en el carácter persistente y transversal de la desigualdad de género–, de las cadenas mundiales de suministro y de las oportunidades de diálogo social. En vista de ello, se proponen también recomendaciones de política en las que se presta especial atención al fortalecimiento de la protección de los trabajadores y de las instituciones representativas, el replanteamiento de los marcos normativos y de los sistemas fiscales, y el logro de transiciones justas.
Combining Opinions for Use in Bayesian Networks: A Measurement Error Approach
Authors: Charisse Farr A. , Mengersen Kerrie , Ruggeri Fabrizio , Simpson Daniel , Wu Paul , Yarlagadda Prasad 
Published: December 2019

SummaryBayesian networks (BNs) are graphical probabilistic models used for reasoning under uncertainty. These models are becoming increasingly popular in a range of fields including engineering, ecology, computational biology, medical diagnosis and forensics. In most of these cases, the BNs are quantified using information from experts or from users' opinions. While this quantification is straightforward for one expert, there is still debate about how to represent opinions from multiple experts in a BN. This paper proposes the use of a measurement error model to achieve this. The proposed model addresses the issues associated with current methods of combining opinions such as the absence of a coherent probability model, the loss of the conditional independence structure of the BN and the provision of only a point estimate for the consensus. The proposed model is applied to a subnetwork (the three final nodes) of a larger BN about wayfinding in airports. It is shown that the approach performs well than do existing methods of combining opinions.
Finite element modeling of resistive surface layers by micro‐contact impedance spectroscopy
Authors: Veazey Richard A. , Gandy Amy S. , Sinclair Derek C. , Dean Julian S. 
Published: December 2019

AbstractMicro‐contact impedance spectroscopy (MCIS) is potentially a powerful tool for the exploration of resistive surface layers on top of a conductive bulk or substrate material. MCIS employs micro‐contacts in contrast to conventional IS where macroscopic electrodes are used. To extract the conductivity of each region accurately using MCIS requires the data to be corrected for geometry. Using finite element modeling on a system where the resistivity of the surface layer is at least a factor of ten greater than the bulk/substrate, we show how current flows through the two layers using two typical micro‐contact configurations. This allows us to establish if and what is the most accurate and reliable method for extracting conductivity values for both regions. For a top circular micro‐contact and a full bottom counter electrode, the surface layer conductivity (σs) can be accurately extracted using a spreading resistance equation if the thickness is ~10 times the micro‐contact radius; however, bulk conductivity (σb) values can not be accurately determined. If the contact radius is 10 times the thickness of the resistive surface, a geometrical factor using the micro‐contact area provides accurate σs values. In this case, a spreading resistance equation also provides a good approximation for σb. For two top circular micro‐contacts on thin resistive surface layers, the MCIS response from the surface layer is independent of the contact separation; however, the bulk response is dependent on the contact separation and at small separations contact interference occurs. As a consequence, there is not a single ideal experimental setup that works; to obtain accurate σs and σb values the micro‐contact radius, surface layer thickness and the contact separation must all be considered together. Here we provide scenarios where accurate σs and σb values can be obtained that highlight the importance of experimental design and where appropriate equations can be employed for thin and thick resistive surface layers.
Overexpression of B7H5/CD28H is associated with worse survival in human gastric cancer
Authors: Hu Can , Xu Zhiyuan , Chen Shangqi , Lv Hang , Wang Yiping , Wang Xiaofeng , Mo Shaowei , Shi Chengwei , Wei Shenyu , Hu Liqiang , Chen Wei , Cheng Xiangdong 
Published: December 2019

AbstractGastric cancer (GC) is a common malignancy with low 5‐year overall survival (OS). Recently, immune therapy has been used to treat cancer. B7H5 and CD28H are novel immune checkpoint molecules. However, the prognostic value of B7H5/CD28H expression in patients with GC remains unclear. In this study, seventy‐one patients diagnosed with GC were included in this study. Patients' GC tissues and matched adjacent tissue constructed a tissue microarray. The expression levels of B7H5 and CD28H were examined using immunohistochemistry. Correlations between the expression of B7H5 and CD28H and the clinical data were evaluated. We found that the expression of B7H5 and CD28H (both P = .001) were higher in GC tumour tissues than in adjacent noncancerous tissues. B7H5/CD28H expression acted as an independent predictive factor in the OS of patients with GC. High expression of B7H5 and CD28H predicted poor outcome. Patients in the B7H5+CD28H+ group had a lower 5‐year OS compared with patients in the B7H5−CD28− group (4.5% vs 55.6%, P = .001). A significant difference was found in the 5‐year OS between patients in the B7H5+CD28H− and B7H5+CD28H+ groups (33.5% vs 4.5%, P = .006). However, there was no correlation between B7H5 and CD28H expression (P = .844). Therefore, B7H5 and CD28H expression are up‐regulated in GC and are independent prognostic factors for overall survival in patients with GC. Although there was no correlation between B7H5 and CD28H expression, high expression of B7H5 and CD28H predicts poor prognosis, especially when both are highly expressed.
Safety and the effectiveness of a new education program for nurses to assess swallowing function using fiberoptic endoscopic evaluation of swallowing (FEES)
Authors: Yoshida Mikako , Kagaya Hitoshi , Kamakura Yayoi , Miura Yuka , Saitoh Eiichi , Okawa Yohei , Sanada Hiromi 
Published: December 2019

AbstractAimNurses have the potential to perform fiberoptic endoscopic evaluation of swallowing (FEES) in Japan, which can aid in their provision of swallowing care appropriate to individual patients' swallowing functions. We aimed to confirm the safety and effectiveness of a new nursing education program on FEES by examining its impact on autonomy in performing FEES and accuracy in observing bolus swallowing.MethodsIn this prospective descriptive study, we developed a FEES education program comprising three phases: (a) obtaining knowledge and skills through e‐leaning and a practical workshop; (b) becoming proficient in skills for assessing healthy adults through self‐learning; and (c) obtaining clinical competence through 30 cases of on‐the‐job training (OJT). The trends in autonomy and in accuracy of FEES were evaluated by the data during OJT.ResultsOf the three certified nurses in dysphagia nursing, a trainee completed 30 cases of OJT and the other two trainees experienced 20 cases and 10 cases, respectively without any adverse event. Autonomy in the four major FEES skills gradually increased over the OJT. The correct answer rate, sensitivity, and specificity of the penetration‐aspiration scale and severity of residue in the pyriform sinus and epiglottis valley were above 95% at the first 10 cases of OJT among three trainees and they were 100% after the 11th case of OJT.ConclusionsOur results suggest that the developed education program helped nurses with experience being present at FEES obtain sufficient knowledge and skills to appropriately and safely perform FEES with 30 cases of OJT.
Prenatal electronic cigarette exposure decreases brain glucose utilization and worsens outcome in offspring hypoxic–ischemic brain injury
Authors: Sifat Ali E. , Nozohouri Saeideh , Villalba Heidi , Al Shoyaib Abdullah , Vaidya Bhuvaneshwar , Karamyan Vardan T. , Abbruscato Thomas 
Published: December 2019

AbstractIt has been shown that prenatal nicotine and tobacco smoke exposure can cause different neurobehavioral disorders in the offspring. We hypothesize that prenatal exposure to nicotine‐containing electronic cigarette (e‐Cig) vapor can predispose newborn to enhanced sensitivity to hypoxic–ischemic (HI) brain injury and impaired motor and cognitive functions. In this study, pregnant CD1 mice were exposed to e‐Cig vapor (2.4% nicotine). Primary cortical neurons isolated from e‐Cig exposed fetus were exposed to oxygen–glucose deprivation followed by reoxygenation (OGD/R) to mimic HI brain injury. Cell viability and glucose utilization were analyzed in these neurons. HI brain injury was induced in 8–9‐day‐old pups. Short‐term brain injury was evaluated by triphenyltetrazolium chloride staining. Long‐term motor and cognitive functions were evaluated by open field, novel object recognition, Morris water maze, and foot fault tests. Western blotting and immunofluorescence were done to characterize glucose transporters in offspring brain. We found that e‐Cig exposed neurons demonstrated decreased cell viability and glucose utilization in OGD/R. Prenatally e‐Cig exposed pups also had increased brain injury and edema 24 hr after HI brain injury. Further, in utero e‐Cig exposed offspring with HI brain injury displayed impaired memory, learning, and motor coordination at adolescence. Additionally, the expression of glucose transporters decreased in e‐Cig exposed offspring brain after HI brain injury. These results indicate that reduced glucose utilization can contribute to prenatal e‐Cig exposure induced worsened HI brain injury in offspring. This study is instrumental in elucidating the possible deleterious effects of e‐Cig use in the general population.
image
Early identification of cardiac autonomic neuropathy using complexity analysis in children with type 1 diabetes
Authors: Kane Penny , Larsen Peter , Wiltshire Esko 
Published: December 2019

AimIn children and adolescents, there are significant limitations to detecting cardiac autonomic neuropathy (CAN), an important contributor to morbidity and mortality in adults with type 1 diabetes (T1D). The analysis of heart rate variability (HRV) is one method available to detect CAN. Some evidence shows traditional linear HRV measures detect abnormalities in youth with T1D. In this study, we aimed to assess whether non‐linear complexity analysis of HRV would assist identification of CAN in youth with T1D and to assess contributory factors.MethodsWe studied 19 youth with T1D and 17 healthy controls. Each had an electrocardiogram recorded continuously for 10 min, at a sampling frequency of 1000 Hz. Using Labview software and an algorithm for complexity analysis, along with standard time‐domain and spectral analysis, recordings of the electrocardiogram were analysed to detect differences in HRV between groups.ResultsYouth with T1D had significantly higher sample entropy than controls (P = 0.015) suggesting increased complexity in HRV, but similar detrended fluctuation analysis (P = 0.68). Youth with T1D also had increased % high frequency power (P = 0.017) and reduced mid‐frequency power (P = 0.019) on spectral analysis. There were no differences in heart rate or blood pressure responses to standing, or time‐domain analysis of HRV. Within the T1D group, sample entropy correlated strongly with triglycerides (r = 0.76, P = 0.001) and detrended fluctuation analysis correlated strongly with serum potassium (r = −0.86, P < 0.001).ConclusionsComplexity analysis of HRV, particularly using sample entropy, may aid detection of CAN in youth with T1D.