bluuebunny/crossref_metadata_2025_split huggingface dataset:
The potential interaction of hereditary and reproductive factors in the etiology of mammary gland hyperplasia: a case-control study
Authors: gao hanlu , Yang Chao , Fan Jinqing , Lan Li , Pang Da 
Published: December 2019

Abstract
Background: Family history of breast cancer and female reproductive factors may work together to influence hyperplasia of mammary gland (HMG) risk. However, the association with HMG risk is poorly characterized and might be important to understand the causation of HMG.Methods: A total of 1881 newly diagnosed HMG cases and 1900 controls were recruited between 2012 and 2017. We collected each participant's demographic characteristics, female reproductive factors and family history of breast cancer. A multi-analytic strategy combining unconditional logistic regression, multifactor dimensionality reduction (MDR) and crossover approaches were applied to systematically identify the interactions of family history of breast cancer and reproductive factors on HMG susceptibility.Results: In MDR analysis, high-order interactions among education level, breastfeeding duration and family history of breast cancer were identified among women (OR=7.069, 95%CI: 6.080-8.219). Similarly, in crossover analysis, compared with individuals having low education level and no family history of breast cancer, HMG risk increased significantly for those having high education level and family history of breast cancer (OR=36.389, 95%CI: 11.469-115.451), similar additive interaction effect was observed among short breastfeeding duration women (OR=27.699, 95%CI: 3.730-205.699).Conclusion: This study suggests high-order interactions of high education level, short breastfeeding duration and family history of breast cancer may synergistically increased HMG risk.
Perforator Flap Based Technique for the Treatment of Dupuytren's Contracture
Authors: Cooper Tokai B , Zhao Bin , Chen Xinglong , Li Zhijie , Gao Weiyang , Yan Hede 
Published: December 2019

Abstract
Background: Perforator flap based technique was used in treating Dupuytren’s Contracture in a cohort of 48 patients. This perforator based on the ulnar palmar digital artery originates from the superficial palmar arch and supplies the hypothenar area. Methods: A curved incision that exposes the diseased palmar fascia was made in middle to distal palm lateral to the hypothenar eminence beginning 20mm distal to the distal wrist crease up to the heel of the palm. An additional incision from the arch of the curved incision extends into the middle phalanx for exposure of the digital cord. The perforator flap was raised along the hypothenar region in 53 hands of 48 patients, nine females and 39 males and their ages at the time of surgery averaged 56 years. The Tubiana classification illustrates the extent of the disease in our patients’ population with no distal interphalangeal joint involvement. Results: Of the 48 patients, five patients had bilateral hands involvement. Two patients complained of paresthesia in the ring and little fingers after surgery, the symptom had disappeared without further intervention before the latest follow-up. There was no incidence of skin necrosis and delayed healing. Up to date, there has been no reported recurrence. Conclusion: This perforator flap based technique is technically simple and reliable with better exposure and easier removal of all the diseased fascia, making it possible for primary healing without skin necrosis and acceptable for treatment of patients at all stages of the disease.
Hypoxic hepatocellular carcinoma cells acquire arsenic trioxide resistance through upregulating HIF-1α expression
Authors: Chen Yaoting , Li Huiqing , Chen Dong , Jiang Xiongying , Wang Weidong , Li Dan , Shan Hong 
Published: December 2019

Abstract
Background: Although arsenic trioxide (ATO) is used in treatment of advanced hepatocellular carcinoma (HCC) in clinical trials, it is not yet satisfied in improving HCC patients’ overall survival. Intratumoral hypoxia and overexpression of hypoxia-inducible factor-1α (HIF-1α) may result in ATO-resistance and tumor progression. We investigated the mechanisms between HIF-1α expression and acquired ATO-chemoresistance in HCC cells and in mice.
Methods: Therapeutic effects of ATO in normoxic and hypoxic HCC cells were assessed using cell viability and apoptosis assays in vitro and xenografts model. mRNA and protein expression of HIF-1α, P-glycoprotein, and VEGF were measured by qRT-PCR and western blotting. HIF-1α inhibition was performed to investigate the mechanism of ATO-resistance. VEGF secretion was tested using ELISA and tube-formation assay.
Results: Hypoxic HCC cells showed more resistance to ATO, with higher IC50 values and less apoptosis, than normoxic cells and upregulated HIF-1α protein expression, accompanied with the enhancement of P-glycoprotein and VEGF synthesis after ATO treatment. VEGF secretion was elevated in ATO-treated supernatant to potentiate angiogenesis in vitro. HIF-1α inhibition attenuated ATO-resistance and angiogenesis, and promoted the anticancer effects of ATO both in vitro and in vivo by downregulating therapy-induced P-glycoprotein and VEGF overexpression.
Conclusions: Hypoxic HCC cells acquire ATO-resistance by upregulating HIF-1α levels; thus ATO combined with targeting HIF-1α levels may lead to the enhanced antitumor effects in HCC.
Keywords: hepatocellular carcinoma, arsenic trioxide, drug resistance, HIF-1α, targeted therapy
Geographical&nbsp;Accessibility&nbsp;and&nbsp;Spatial&nbsp;Coverage&nbsp;Modelling&nbsp;of&nbsp;Public&nbsp;Health&nbsp;Care&nbsp;Network&nbsp;in&nbsp;Rural&nbsp;and&nbsp;Remote&nbsp;India.
Authors: Verma Veenapani Rajeev , Dash Umakant 
Published: December 2019

Abstract
Background: Long distances to facilities, topographical constraints, inadequate service capacity of institutions and insufficient/ rudimentary road & transportation network culminate into unprecedented barriers to access. These barriers gets exacerbated in presence of external factors like conflict and political disruptions. Thus, this study was conducted in rural, remote and fragile region in India measuring geographical accessibility and modelling spatial coverage of public healthcare network. Methods: Vector and raster based approaches were used to discern accessibility for various packages of service delivery. Alternative scenarios derived from local experiences were modelled using health facility, population and ancillary data. Based on that, a raster surface of travel time between facilities and population was developed by incorporating terrain, physical barriers, topography and travelling modes and speeds through various land-cover classes. Concomitantly, spatial coverage was modelled to delineate catchment areas. Further, underserved population and zonal statistics were assessed in an interactive modelling approach to ascertain spatial relationship between population, travel time and zonal boundaries. Finally, raster surface of travel time was re-modelled for the conflict situation in villages vulnerable to obstruction of access due to disturbed security scenario. . Results: Euclidean buffers revealed 11% villages without ambulatory & immunization care within 2 km radius. Similarly, for 5 km radius, 11% and 12% villages were bereft of delivery and inpatient care. Travel time accessibility analysis divulged walking scenario exhibiting lowest level of accessibility. Enabling motorized travel improved accessibility measures, with highest degree of accessibility for privately owned vehicle (motorcycle and cars). Differential results were found between packages of services where ambulatory & immunization care was relatively accessible by walking; whereas, delivery and inpatient care had a staggering average of three hours walking time. Even with best scenario, around 2/3rd population remained unserved for all package of services. Moreover, 90% villages in conflict zone grapples with inaccessibility when the scenario of heightened border tensions was considered. Conclusions: Our study demonstrated the application of GIS technique to facilitate evidence backed planning at granular level. The analysis divulged inaccessibility to delivery and inpatient care to be most pronounced and majority of population to be unserved, regardless of the scenario. It was suggested to have concerted efforts to bolster already existing facilities and adapt systems approach to exploit synergies of inter-sectoral development.
PROKNOW-C: DA SELEÇÃO DE UM PORTFÓLIO DE ARTIGOS A ANÁLISE SISTÊMICA SOBRE BLOCOS DE TERRA COMPRIMIDA
Authors: França Sâmara , Schuab Mariana Rezende , Sperandio Kastelli Pacheco , Azevedo Rogério Cabral de , Carvalho Maria Cristina Ramos de , Bezerra Augusto Cesar da Silva 
Published: December 2019

Blocos de terra compactada são elementos de alvenaria feitos a partir de terra estabilizada e adensada. Estes materiais se destacam no quesito sustentabilidade por dispensarem a fase de queima em sua produção. A estabilização química dos blocos pode ser realizada através da ativação alcalina que consiste na obtenção de ligantes a partir de materiais aluminossilicatos em uma solução fortemente alcalina. A ativação alcalina também é vista como ambientalmente correta devido a sua baixa emissão de gases do efeito estufa. Entre os materiais precursores da ativação alcalina estão as cinzas volantes devido a sua composição química. Diante desse contexto, o objetivo deste trabalho é montar um portfólio de artigos científicos sobre blocos de terra compactado com adição de cinzas orgânicas e ativação alcalina, fazer análise bibliométrica e sistêmica dos artigos. O portfólio bibliográfico resultante é composto por 4 artigos relevantes e alinhados com o tema. Dentro da análise bibliométrica pode-se destacar o artigo “Feasibility o fmanufacturing geopolymer bricks using circulating fluidized bed combustion bottom ash” como mais relevante. Em relação a análise sistêmica elencou-se 5 lentes para a discussão dos artigos e a partir disso pode-se observar oportunidades para futuras pesquisas.
Mineral and Amino Acid Composition of Germinated and Canned Wheat Grains
Authors: Zenkova Maria 
Published: December 2019

Introduction. Grain germination can improve the nutritional value and functional properties of grain. The research featured the effect of technological processing on the quality of the finished product as in the case of natural, germinated, and canned wheat grains. A set of experiments was conducted to define the mineral substances and amino acid composition as factors that affect the chemical composition of a product during its production.
Study objects and methods. The research featured soft wheat grain, germinated wheat grain, and canned germinated wheat grain. The latter consisted of 55% of pre-treated germinated wheat grain and 45% of filling, which contained 4.5% of sugar and 3.5% of salt. The content of mineral substances was determined by atomic-emission spectrometry with inductively coupled plasma using an atomicemission spectrometer Optima 2100 DV (USA). The amino acid composition was determined using a liquid chromatograph Agilent 1200 (USA).
Results and discussion. 100 grams of canned germinated grain contained about 15% of daily manganese, 12% of chromium (III), 8.4% of copper, and 8.3% of zinc. Canned germinated wheat demonstrated a high biological protein value and contained 20.6% of essential amino acids. As for amino acids, canned germinated wheat grains had a high content of proline (958.4 mg/100 g) and glutamic acid (2456.4 mg/100 g). The characteristics of the nutritional value of canned protein from germinated wheat grains included amino acid rate, amino acid feedstock difference coefficient (AAFDC), and potential biological value (PBV). The proteins of canned germinated wheat appeared inferior in their amino acid composition and contained insufficient amounts of lysine and threonine. The coefficient of differences in amino acid scores was 71.9%, while the potential biological value of the protein was 28.1%.
Conclusion. Germinated grains have a certain set of minerals and amino acids in their composition. Therefore, they can be used to develop canned foods or dishes for public catering industry. The effect of technological processing on the mineral composition of the finished product resulted in the loss of mineral substances from 45 to 85%. Moreover, germinated wheat should be combined with products that are rich in lysine and threonine.
Extraction of Plant Pigments from Local Raw Materials
Authors: Kostina Natalya , Podlegaeva Tatiana , Sergeeva Irina 
Published: December 2019

Introduction. Violation of the ecological balance and modern processing methods may result in a lower content of important nutrients in food. Therefore, there is a need to restore the balance of biologically active substances in food products. In this regard, it seems appropriate to use plant materials that contain biologically active substances, as well as pigments. The use of aromatic plants for these purposes remains understudied. The research objective was to extract chlorophyll-based green pigments based from green plants.
Study objects and methods. The object of the study was dried parsley (Petroselinum vulgare). The extractants included acetone, ethyl alcohol (95%), hexane, petroleum ether, and chloroform.
Results and discussion. The extractants were chosen according to the total content of chlorophylls, which determine the color of the extract. Since carotenoids are extracted along with chlorophylls, the content of this component was also determined. Acetone demonstrated the maximum degree of chlorophyll recovery. However, this solvent cannot be used in food industry. Therefore, 95% ethanol, which also showed a high degree of chlorophyll extraction, was chosen as the extractant. A set of experiments made it possible to establish the ratio of feedstock and extractant as 1:60 (three times extraction with a mass ratio of parsley and extract); the optimal time of each extraction was 0.5 ± 0.1 h. Magnesium carbonate (MgCO2) helped to preserve the dyes. In order to increase the concentration of coloring substances, the obtained extract was subjected to thickening at a residual pressure of 400 Pa at 40°C.
Conclusion. The obtained concentrate from dried parsley can be used in production of combined foods.
Fatty Acid Composition of Goat and Sheep Milk: Transformation during Yogurt Production
Authors: Sadovoy Vladimir , Voblikova Tatyana , Permyakov Anatoly 
Published: December 2019

Introduction. Information on the structure of fatty acids is crucial for production and promotion of goat and sheep milk in dairy industry. The profile of fatty acids of milk fat can affect the nutrition value and market value of dairy products.
Study objects and methods. The present research featured fatty acid structure of goat and sheep milk and its transformation during yogurt production. The fatty acid structure was studied using gas chromatography. The milk was obtained from goats of the Zaanensky breed and sheep of the North Caucasian breed. Their ratio in the yogurt was 1:1.
Results and discussion. The content of saturated fatty acids was 12% less in goat milk than in sheep milk. Olein, stearin, and palmitic acids are the main fatty acids in the fatty phase of milk and yogurt. The research revealed some changes in concentration of individual fatty acids during milk processing and during the storage of yogurt. As a result of ripening and storage, the amount of saturated fatty acids in yogurt increased by 5% on day 7, in comparison with the initial dairy mix. The content of the polynonsaturated fatty acids decreased by 19.27%. Goat milk had the highest value of the ratio of the hypocholesteremic and hypercholesteremic fatty acids. Goat milk demonstrated the most acceptable fatty acid structure in terms of healthy nutrition and prevention of atherosclerosis and thrombogenesis. During yogurt production and storage, the monononsaturated and polynonsaturated fatty acids decreased, while the content of saturated fatty acids increased. Thus, goat milk can increase the amount of monononsaturated and polynonsaturated fatty
acids in dairy products. However, the research also revealed a general tendency to decrease in monononsaturated and polynonsaturated fatty acids during yogurt production and storage, with a parallel increase in the content of saturated fatty acids. Unlike ship milk, goat milk had a lower value of the indices of atherogenicity and thrombogenesis.
Conclusion. The indices of atherogenicity and thrombogenesis provided additional information on the functional properties of the product. The established features of the fatty acid profile and its transformation during yogurt production provide data that can help produce qualitatively new dairy drinks with a healthy fatty acid profile.
The Current State and Prospects of the Grain Market in the Kemerovo Region
Authors: Shadrin Vladislav , Kotova Olga , Polikarpova Lada 
Published: December 2019

Introduction. Kuzbass is a large territorial complex with highly-developed industries. However, the local agriculture is also quite intensive. The research objective was to summarize the results various studies of the current state of the grain market in the Kemerovo region and identify areas of its development. Implementation of the grain complex development strategy with marketing elements will strengthen the position of the Kemerovo region on the international market of grain and products of its processing.
Study objects and methods. The research was based on a systematic approach to the study of the regional grain market and employed abstract-logical, statistical-economic, and graphical research methods.
Results and discussion. The article focuses on the current state of Kemerovo grain market and identifies development prospects and sale opportunities. In recent years, the volume of wheat and rye yield has significantly increased, as well as the canola acreage. The main consumer of grain crops grown in the Kemerovo region is the region itself. Nevertheless, the region is gradually becoming a grain exporter. The authors identified factors that affect the enterprises specializing in the production and sale of grain. The region has a good base for the development of agriculture in general and grain crops in particular. Unfortunately, the current problems in cultivation technologies, price fluctuations, insufficient state support, and poor marketing hinder the development of the industry. The authors proposed several directions of grain market development proceeding from possibilities of producers and change in the structure of production sales taking into account foreign trade risks.
Conclusion. Marketing and realization of grain production in the Kemerovo region should be based on a well-planned development strategy of improving the competitive positions of the region and the development of a large production and technological complex.
Exosomal miR-1298 and lncRNA-RP11-583F2.2 Expression in Hepatocellular Carcinoma
Authors: Matboli Marwa , Labib Mariam E. , Nasser Hanaa El-Tayeb , El-Tawdi Ahmed H.F. , Habib Eman K. , Ali-Labib Randa 
Published: December 2019

Aim:
The aim of this study was to explore the expression of exosomal non-coding RNAs
(ncRNAs) in the sera of patients with HCC versus control.

Methods:
Firstly, Bioinformatics analysis was conducted to retrieve ncRNAs specific to HCC (hsamiRNA-
1298 and lncRNA-RP11-583F2.2). Afterwards, extraction and characterization of exosomes
were performed. We measured the expression of the chosen exosomal RNAs by reverse transcriptase
quantitative real-time PCR in sera of 60 patients with HCC, 42 patients with chronic hepatitis C
(CHC) infection and 18 healthy normal volunteers.

Results:
The exosomal ncRNAs [hsa-miRNA-1298, lncRNA-RP11-583F2.2] had better sensitivity
and specificity than alpha-fetoprotein (AFP) in HCC diagnosis.

Conclusion:
The exosomal hsa-miRNA-1298, lncRNA-RP11-583F2.2 can be potential biomarkers for
HCC diagnosis.
Chemical Composition and In vitro Antidiabetic Effects of Olea europaea Linn. (Olive)
Authors: Ahamad Javed , Uthirapathy Subasini , Ameen Muath S.M. , Anwer Esra T. , Hussain Faiq H.S. , Mir Showkat R. 
Published: December 2019

Background:
Olive oil and fruits are essential components of Mediterranean diets. The olive
tree is a prevalent plant species and one of the important cultivated crops of the Mediterranean region.
The present study aimed to evaluate the effectiveness of olive in achieving glucose homeostasis through
the inhibition of carbohydrate metabolizing enzymes using in vitro models and also determine the chemical
composition of olive oil by GC/MS.

Methods:
The chemical composition of olive oil was determined by GC/MS and its antidiabetic activity
was assessed through inhibition α-amylase and α-glucosidase enzymes in in vitro models.

Results:
The olive oil analysis by GC/MS yielded 41 constituents amounting to 98.21% of total oil
composition. Oleic acid, 3-(octadecyloxy) propyl ester (19.34%), arachidonic acid (11.25%), oleic acid
(6.07%), Docosahexaenoic Acid (DHA) (9.50%), pentadecanoic acid (5.53%), palmitic acid (3.86%),
and linoleic acid (3.13%) were the major components of olive oil. Olive oil and extract produce dosedependent
inhibition of α-amylase and α-glucosidase enzymes. The IC50 values for olive oil, olive extract,
and acarbose were found as 210.50±4.76, 121.8±3.18, and 91.04±2.16 μg/mL, respectively,
against the α-amylase enzyme. The IC50 values for olive oil, extract, and acarbose were found as
204.3±3.41, 165.04±5.27 and 116.5±2.17 μg/mL, respectively, against the α-glucosidase enzyme.

Conclusion:
The result of this study concluded that olive oil has oleic acid and its ester derivatives as
major constituents. The study findings also confirm the traditional claim of olive use in the treatment of
diabetes mellitus.
Facile Synthesis of Cu2O nanoparticle-loaded Carbon Nanotubes Composite Catalysts for Reduction of 4-Nitrophenol
Authors: Feng Yao , Wang Ran , Yin Juanjuan , Zhan Fangke , Chen Kaiyue , Jiao Tifeng , Zhou Jingxin , Zhang Lexin , Peng Qiuming 
Published: December 2019

Background:
4-nitrophenol (4-NP) is one of the pollutants in sewage and harmful to human
health and the environment. Cu is a non-noble metal with catalytic reduction effect on nitro
compounds, and.has the advantages of simple preparation, abundant reserves, and low price. Carbon
nanotubes (CNT) are widely used for substrate due to their excellent mechanical stability and high
surface area. In this study, a simple method to prepare CNT-Cu2O by controlling different reaction
time was reported. The prepared nanocomposites were used to catalyze 4-NP.

Methods:
CNTs and CuCl2 solution were put into a beaker, and then ascorbic acid and NaOH were
added while continuously stirring. The reaction was carried out for a sufficiently long period of time
at 60°C. The prepared samples were dried in a vacuum at 50°C for 48 h after washing with ethyl
alcohol and deionized water.

Results:
Nanostructures of these composites were characterized by scanning electron microscope and
transmission electron microscopy techniques, and the results at a magnification of 200 nanometers
showed that Cu2O was distributed on the surface of the CNTs. In addition, X-ray diffraction was
performed to further confirm the formation of Cu2O nanoparticles. The results of ultraviolet spectrophotometry
showed that the catalytic effect of the compound on 4-NP was obvious.

Conclusions:
CNTs acted as a huge template for loading Cu2O nanoparticles, which could improve
the stability and cycle performance of Cu2O. The formation of nanoparticles was greatly affected by
temperature and the appropriate concentration, showing great reducibility for the 4-NP reduction
reaction.