bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ferric Ion Driven Assembly of Catalase‐like Supramolecular Photosensitizing Nanozymes for Combating Hypoxic Tumors
Authors: Li Yongxin , Sun Pan , Zhao Luyang , Yan Xuehai , Ng Dennis K. P. , Lo Pui‐Chi 
Published: September 2020

AbstractA facile approach to assemble catalase‐like photosensitizing nanozymes with a self‐oxygen‐supplying ability was developed. The process involved Fe3+‐driven self‐assembly of fluorenylmethyloxycarbonyl (Fmoc)‐protected amino acids. By adding a zinc(II) phthalocyanine‐based photosensitizer (ZnPc) and the hypoxia‐inducible factor 1 (HIF‐1) inhibitor acriflavine (ACF) during the Fe3+‐promoted self‐assembly of Fmoc‐protected cysteine (Fmoc‐Cys), the nanovesicles Fmoc‐Cys/Fe@Pc and Fmoc‐Cys/Fe@Pc/ACF were prepared, which could be disassembled intracellularly. The released Fe3+ could catalyze the transformation of H2O2 enriched in cancer cells to oxygen efficiently, thereby ameliorating the hypoxic condition and promoting the photosensitizing activity of the released ZnPc. With an additional therapeutic component, Fmoc‐Cys/Fe@Pc/ACF exhibited higher in vitro and in vivo photodynamic activities than Fmoc‐Cys/Fe@Pc, demonstrating the synergistic effect of ZnPc and ACF.
Nonlinear tensile behavior of cotton fabric reinforced polypropylene composites
Authors: Shao Bo , Chen Bo , Cao Yan , Fang Yiqun , Song Yongming , Wang Qingwen 
Published: September 2020

AbstractFrom the perspectives of elastoplasticity (nontime‐dependent) and viscoelasticity (time‐dependent), the Ramberg–Osgood relation and time‐varying viscosity Maxwell (TVM) models were used to model and analyze the stress–strain behavior of cotton fabric‐reinforced polypropylene composites (CFRLs), respectively. The Ramberg–Osgood relation could well describe the tensile behavior of CFRLs as an elastoplastic behavior, while the tensile behavior could also be described as a nonlinear viscoelasticity behavior by Maxwell model. The fitting results showed that the Maxwell model accurately described the tensile behavior of different CFRLs samples under low strain, but there was a considerable gap between the test data and model values when the strain was greater than 5%. Therefore, a time‐varying viscosity fluid damper was used instead of a Newtonian fluid damper to modify the Maxwell model, namely the TVM model. The TVM model closely described the stress–strain behavior during the entire tensile process.
Effects of genetically modified human skin fibroblasts, stably overexpressing hepatocyte growth factor, on hepatic functions of cocultured C3A cells
Authors: Wencel Agnieszka , Ciezkowska Malgorzata , Wisniewska Monika , Zakrzewska Karolina E. , Pijanowska Dorota G. , Pluta Krzysztof D. 
Published: September 2020

AbstractDiseases leading to terminal hepatic failure are among the most common causes of death worldwide. Transplant of the whole organ is the only effective method to cure liver failure. Unfortunately, this treatment option is not available universally due to the serious shortage of donors. Thus, alternative methods have been developed that are aimed at prolonging the life of patients, including hepatic cells transplantation and bridging therapy based on hybrid bioartificial liver devices. Parenchymal liver cells are highly differentiated and perform many complex functions, such as detoxification and protein synthesis. Unfortunately, isolated hepatocytes display a rapid decline in viability and liver‐specific functions. A number of methods have been developed to maintain hepatocytes in their highly differentiated state in vitro, amongst them the most promising being 3D growth scaffolds and decellularized tissues or coculture with other cell types required for the heterotypic cell‐cell interactions. Here we present a novel approach to the hepatic cells culture based on the feeder layer cells genetically modified using lentiviral vector to stably produce additional amounts of hepatocyte growth factor and show the positive influence of these coculture conditions on the preservation of the hepatic functions of the liver parenchymal cells' model—C3A cells.
Recombinant protein production‐associated metabolic burden reflects anabolic constraints and reveals similarities to a carbon overfeeding response
Authors: Li Zhaopeng , Rinas Ursula 
Published: September 2020

AbstractA comparison of the metabolic response of Escherichia coli BL21 (DE3) towards the production of human basic fibroblast growth factor (hFGF‐2) or towards carbon overfeeding revealed similarities which point to constraints in anabolic pathways. Contrary to expectations, neither energy generation (e.g., ATP) nor provision of precursor molecules for nucleotides (e.g., uracil) and amino acids (e.g., pyruvate, glutamate) limit host cell and plasmid‐encoded functions. Growth inhibition is assumed to occur when hampered anabolic capacities do not match with the ongoing and overwhelming carbon catabolism. Excessive carbon uptake leads to by‐product secretion, for example, pyruvate, acetate, glutamate, and energy spillage, for example, accumulation and degradation of adenine nucleotides with concomitant accumulation of extracellular hypoxanthine. The cellular response towards compromised anabolic capacities involves downregulation of cAMP formation, presumably responsible for subsequently better‐controlled glucose uptake and resultant accumulation of glucose in the culture medium. Growth inhibition is neglectable under conditions of reduced carbon availability when hampered anabolic capacities also match with catabolic carbon processing. The growth inhibitory effect with accompanying energy spillage, respectively, hypoxanthine secretion and cessation of cAMP formation is not unique to the production of hFGF‐2 but observed during the production of other proteins and also during overexpression of genes without transcript translation.
The impact of stress, anxiety and depression on stomatognathic system of physiotherapy and dentistry first‐year students
Authors: Owczarek Joanna Elżbieta , Lion Katarzyna Małgorzata , Radwan‐Oczko Małgorzata 
Published: August 2020

AbstractIntroductionIt is well proven that psychoemotional factors play causative role in development of many civilization diseases. Psychosocial stress is effecting with development of certain oral parafunctions like teeth grinding, bruxism, or cheeks biting. Eventually, all of those parafunctional activities may cause increase of masticatory muscles tone and provoke its intensification. Literature indicates that students must cope with greater level of stressful situations than the average representative of the society.MethodsThe research group comprised Ist‐year physiotherapy and dentistry students from University School of Physical Education in Wroclaw and Wroclaw Medical University. The study consisted the following: psychological and health questionnaires, and stomatological examination with masseter muscles' electromyography.ResultsIn intraoral examination, symptoms of parafunctions were more frequently observed in physiotherapy students. The mean tone of masseters was higher also among physiotherapy students. The level of perceived stress was high in both groups, and the experienced borderline and incorrect results in Hospital Anxiety and Depression Scale were more frequently observed in dentistry students. The mean tone of masseter muscle was rising with the level of anxiety in physiotherapy group. Among dentistry, the tendency for rise of masseter muscle tone together with level of anxiety and depression was observed.ConclusionsPhysiotherapy and dentistry beginners differ between each other's by prevalence of temporomandibular disorders (TMD) and oral parafunction symptoms. The level of perceived stress is high in both groups. The level of experienced anxiety and depression is higher in dentistry group. In both study groups, there is significant growth and tendency for simultaneous rise of masseter muscle tone accordingly to stress, anxiety, and depression indicators.
Knockdown of lncRNA ZNRD1‐AS1 inhibits progression of bladder cancer by regulating miR‐194 and ZEB1
Authors: Gao Zhixiang , Li Shidong , Zhou Xufeng , Li Huali , He Shasha 
Published: August 2020

AbstractBackgroundBladder cancer (BC) is a common urinary neoplasm with high incidence worldwide. Long noncoding RNA zinc ribbon domain containing 1 antisense RNA 1 (ZNRD1‐AS1) has been reported to be upregulated in BC. However, the exact role of ZNRD1‐AS1 as well as its mechanism remains poorly understood.MethodsZinc ribbon domain containing 1 antisense RNA 1, and its potential downstream genes microRNA‐194 (miR‐194) and zinc finger E‐box binding homeobox 1 (ZEB1) levels were detected via quantitative real‐time polymerase chain reaction or western blot. Cell proliferation, migration, invasion, and epithelial‐mesenchymal transition (EMT) were detected to assess the influences of ZNRD1‐AS1, miR‐194 and ZEB1 on BC cells by colony formation, cell counting kit‐8 (CCK‐8), transwell analysis or western blot. The relationship between miR‐194 and ZNRD1‐AS1 or ZEB1 was analyzed by luciferase activity analysis. The xenograft experiment was performed to assess the function of ZNRD1‐AS1 in vivo.ResultsZinc ribbon domain containing 1 antisense RNA 1level was upregulated in BC. ZNRD1‐AS1 silence repressed proliferation, migration, invasion and EMT in BC cells. MiR‐194 was identified as a target of ZNRD1‐AS1, and miR‐194 upregulation repressed proliferation, migration, invasion, and EMT by ZNRD1‐AS1 sponging. ZEB1 was targeted via miR‐194 and its interference impeded proliferation, migration, invasion, and EMT. Moreover, ZNRD1‐AS1 regulated ZEB1 expression via miR‐194. Besides, inhibition of ZNRD1‐AS1 attenuated tumor growth by miR‐194/ZEB1 axis in vivo.ConclusionKnockdown of ZNRD1‐AS1 suppressed BC cell development in vitro and in vivo via targeting miR‐194 to regulate ZEB1, indicating a novel avenue for treatment of BC.
Amnion–Chorion Allograft Barrier Used on Root Surface for Regenerative Procedures: Case Report
Authors: Hamada Yusuke , Yeh Yu‐Ting , Blanchard Steven B. 
Published: August 2020

IntroductionGuided tissue regeneration (GTR) has been well documented with combination of bone graft substitutes and biologic modifiers to improve the outcomes of periodontal regenerative procedures. Amnion‐chorion allograft membrane (ACM) is a placenta‐derived resorbable allograft membrane which contains growth factors found in the placenta. The primary purpose of the barrier membranes for GTR was to exclude the epithelial down‐growth along with the root surface, however, the ACM can be used as an additional biologic modifier because of the release of growth factors from the ACM after placement. The aim of this case report is to evaluate the efficacy and the application of ACM on the previously diseased root surface to treat periodontal intrabony defect.Case PresentationA 60‐year‐old Caucasian male with deep and wide intrabony defect on mesial #19 was treated with a regenerative procedure with combination of application of ACM on the root surface and filling the intrabony defect with the corticocancellous freeze‐dried bone allograft. The bone substitute was covered with another layer of ACM and primary closure was achieved. Wound healing process was uneventful, and the clinical and radiographic outcomes were favorable up to 18 months after the surgical procedure.ConclusionThis case report demonstrated that the application of ACM on the root surface with a combination of bone substitute might enhance to the radiographic bone fill and the clinical attachment level gain and minimize the risk of post‐operative gingival recession.
ALDH5A1 acts as a tumour promoter and has a prognostic impact in papillary thyroid carcinoma
Authors: Deng Xing‐Yan , Gan Xiao‐Xiong , Feng Jian‐Hua , Cai Wen‐Song , Wang Xin‐Quan , Shen Liang , Luo Hong‐Tu , Chen Zhen , Guo Mengli , Cao Jie , Shen Fei , Xu Bo 
Published: September 2020

Thyroid cancer is the most common endocrine carcinoma, with papillary thyroid carcinoma (PTC) accounting for 80%‐90% of thyroid cancers. Accumulating studies reported that mitochondria plays an important role in the regulation of cell proliferation. ALDH5A1, may function as an oncogene or tumour suppressor in various human cancers, and the role of ALDH5A1 in PTC is still unclear. The aim of this study was to investigate the clinical significance of ALDH5A1 expression and its functions in PTC. In this present study, we studied ALDH5A1 expression on primary papillary thyroid carcinoma (PTC) in The Cancer Genome Atlas (TCGA) database. Results showed that the levels of ALDH5A1 were found positively correlated with tumour stage, metastasis, lymph node stage, and higher levels of ALDH5A1 demonstrated poor disease‐free survival (DFS). Immunohistochemistry (IHC) revealed that significantly higher expression of ALDH5A1 was found in PTC tissues. On the other hand, knockdown of ALDH5A1 significantly inhibited PTC cell proliferation, migration and invasion detection found the migration and invasion of cells also were hindered when ALDH5A1 level was reduced. The knockdown of ALDH5A1 inhibited the expression of Vimentin and promoted the expression of E‐cadherin. In brief, knockdown of ALDH5A1may act as a novel molecular target for the prevention and treatment of PTC.Significance of the studyThe present study focused on the role and the potential mechanism of ALDH5A1 in papillary thyroid carcinoma. We demonstrated that reduced expression of ALDH5A1 might inhibit the progression of TC by inhibiting cell proliferation, migration and invasion and reversing epithelial‐mesenchymal transition (EMT). The findings ensured the interaction relation between ALDH5A1 and EMT in PTC, providing a novel biological marker for PTC and enriching the potential strategies for TC treatment.
Effect of Turbulence of the Freeboard on Fluidized‐Bed Biomass Combustion
Authors: Wickramasinghe Chathuranga , Narayana Mahinsasa , Amarasinghe A. D. U. Shantha 
Published: September 2020

AbstractFluidized‐bed combustion is a very popular technology used to convert solid biomass into thermal energy, which has many advantages over other conversion technologies. However, these advantages strongly depend on the freeboard combustion. This study focuses on the effect of turbulence of the freeboard via developing a comprehensive mathematical model including four‐way coupling, biomass thermal decomposition, and turbulent combustion by means of an Eulerian‐Lagrangian framework. The validated model predicts with significant accuracy for all gas species when turbulence modeling is improved. The optimum amount of the secondary air ratio and the optimum excess air ratio were determined, which give a higher freeboard temperature while maintaining minimum emission.
Green Precursors and Soft Templating for Printing Porous Carbon‐Based Micro‐supercapacitors
Authors: Lochmann Stefanie , Kintzel Susann , Bräuniger Yannik , Otto Thomas , Zhang En , Grothe Julia , Kaskel Stefan 
Published: September 2020

AbstractA combination of soft lithographic printing and soft templating has been used to fabricate high‐resolution interdigitated micro‐supercapacitors (MSC). Surfactant‐assisted self‐assembly produces high surface area ordered mesoporous carbons (490 m2 g−1). For the first time, such precursors have been printed by nano‐imprint lithography as microdevices with a line width of only 250 nm and a spacing of only 1 μm. The devices are crack‐free with low specific resistance (1.2×10−5 Ωm) and show good device capacitance up to 0.21 F cm−3.
Mechanistic Study of Pd/NHC‐Catalyzed Sonogashira Reaction: Discovery of NHC‐Ethynyl Coupling Process
Authors: Eremin Dmitry B. , Boiko Daniil A. , Kostyukovich Alexander Yu. , Burykina Julia V. , Denisova Ekaterina A. , Anania Mariarosa , Martens Jonathan , Berden Giel , Oomens Jos , Roithová Jana , Ananikov Valentine P. 
Published: September 2020

AbstractThe product of a revealed transformation—NHC‐ethynyl coupling—was observed as a catalyst transformation pathway in the Sonogashira cross‐coupling, catalyzed by Pd/NHC complexes. The 2‐ethynylated azolium salt was isolated in individual form and fully characterized, including X‐ray analysis. A number of possible intermediates of this transformation with common formulae (NHC)nPd(C2Ph) (n=1,2) were observed and subjected to collision‐induced dissociation (CID) and infrared multiphoton dissociation (IRMPD) experiments to elucidate their structure. Measured bond dissociation energies (BDEs) and IRMPD spectra were in an excellent agreement with quantum calculations for coupling product π‐complexes with Pd0. Molecular dynamics simulations confirmed the observed multiple CID fragmentation pathways. An unconventional methodology to study catalyst evolution suggests the reported transformation to be considered in the development of new catalytic systems for alkyne functionalization reactions.
Heterogeneous Parahydrogen‐Induced Polarization of Diethyl Ether for Magnetic Resonance Imaging Applications
Authors: Salnikov Oleg G. , Svyatova Alexandra , Kovtunova Larisa M. , Chukanov Nikita V. , Bukhtiyarov Valerii I. , Kovtunov Kirill V. , Chekmenev Eduard Y. , Koptyug Igor V. 
Published: September 2020

AbstractMagnetic resonance imaging (MRI) with the use of hyperpolarized gases as contrast agents provides valuable information on lungs structure and function. While the technology of 129Xe hyperpolarization for clinical MRI research is well developed, it requires the expensive equipment for production and detection of hyperpolarized 129Xe. Herein we present the 1H hyperpolarization of diethyl ether vapor that can be imaged on any clinical MRI scanner. 1H nuclear spin polarization of up to 1.3 % was achieved using heterogeneous hydrogenation of ethyl vinyl ether with parahydrogen over Rh/TiO2 catalyst. Liquefaction of diethyl ether vapor proceeds with partial preservation of hyperpolarization and prolongs its lifetime by ≈10 times. The proof‐of‐principle 2D 1H MRI of hyperpolarized diethyl ether was demonstrated with 0.1×1.1 mm2 spatial and 120 ms temporal resolution. The long history of use of diethyl ether for anesthesia is expected to facilitate the clinical translation of the presented approach.
Solution‐Processed White Circularly Polarized Organic Light‐Emitting Diodes Based on Chiral Binaphthyl Emitters
Authors: Zhang Yu , Li Jie , Quan Yiwu , Ye Shanghui , Cheng Yixiang 
Published: September 2020

AbstractBy combining the blue and orange CPL or functionalized bis‐benzoxanethones emitters (S‐/R‐BN‐tCz and S‐/R‐BN‐PXZ), warm white CP‐OLEDs were fabricated using solution‐processed single emitting layer strategy. The successful realization of white CP‐EL benefited from the same stable binaphthyl chirality and similar rigid structure of the two emissive CPL emitters. The devices exhibited the low turn‐on voltage of ≈4.3 V, maximum luminance of ≈10200 cd m−2 and maximum current efficiency of ≈2.0 cd A−1. Most significantly, the devices with CIE coordinates of (0.32, 0.45) displayed intense CP‐EL signals in the spectral range of 450 to 650 nm, and showed stable gEL values of ≈10−3 as the luminance increased from 100 to 6000 cd m−2. To the best of our knowledge, this work provides for the first time a simple and feasible strategy to fabricate solution‐processed white CP‐OLEDs based on the co‐doping of the CPL emitters.
Group dialectical behavioral therapy for binge‐eating disorder: Outcomes from a community case series
Authors: Blood Lauren , Adams Gillian , Turner Hannah , Waller Glenn 
Published: September 2020

AbstractObjectiveWhilst there is evidence to support the use of group dialectical behavioral therapy (DBT) in the treatment of binge‐eating disorder (BED), few studies have reported on its effectiveness when delivered in routine clinical practice. This study addressed this gap by exploring the effectiveness of group DBT for BED when delivered in a community eating disorder service.MethodParticipants were 56 adults who presented with BED, and were offered a 20‐week DBT group. Eight groups were conducted. Measures of eating disorder pathology, anxiety, depression and emotion regulation were completed at start and end of treatment, and one‐month follow‐up.ResultsThe attrition rate was 16.1%. Abstinence rates (no objective binges in the previous month) were approximately 60% at the end of treatment and 50% at follow‐up. There were significant reductions in eating disorder psychopathology (but not in mood) by end of treatment and improvements were maintained at follow‐up.DiscussionGroup DBT is an acceptable and effective treatment for adults with BED when delivered in a routine community setting. Findings are broadly comparable with those from research trials. The lack of significant effect on mood suggests that DBT can be effective by teaching new emotion‐regulation skills, rather than changing mood per se.
Detecting tampered region in video using LSTM and U‐Net
Authors: Taya Kunihiko , Kuroki Nobutaka , Takeda Naoto , Hirose Tetsuya , Numa Masahiro 
Published: August 2020

AbstractIn the world today, we can always shoot digital videos with smartphones and share them with family or friends after editing them. It is necessary to check whether these videos have been tampered with to use them as forensic evidence in criminal investigations. This paper proposes a method for detecting tampered regions in a video where a specific subject is removed from a scene. In the proposed method, we use LSTM for the time domain analysis and UNet for the space domain analysis. The detection accuracy achieved 0.98 in terms of F‐measure, even though tampered the region was deformed or moved in the video. The experimental results show superior performance in the detection of tampered regions in digital videos.
The rapid effects of sleeve gastrectomy on glucose homeostasis and resolution of diabetes mellitus
Authors: Bozkurt Emre , Kaya Cemal , Ömeroğlu Sinan , Güven Onur , Mihmanlı Mehmet 
Published: August 2020

AbstractAimsType 2 diabetes caused by obesity is increasing globally. Bariatric surgical procedures are known to have positive effects on glucose homeostasis through neurohormonal action mechanisms. In the present study, we aimed to investigate the factors influencing glucose homeostasis independent of weight loss after the laparoscopic sleeve gastrectomy (LSG).MethodsPatients who underwent LSG for morbid obesity in a 3‐year period were evaluated. Data on demographics, clinical characteristics (duration of diabetes, resected gastric volume, antral resection margin) and laboratory parameters (preoperative and postoperative blood glucose on fasting, preoperative HbA1c levels and first‐year HbA1c levels) were retrospectively reviewed. Effect of patients' body mass index (<50 kg/m2, ≥50 kg/m2), first‐year excess weight loss (EWL%) rates, age (≥50 years, <50 years), duration of diabetes (≥5 years, <5 years) and antral resection margin (≥3 cm, <3 cm) on postoperative blood glucose profile and diabetic resolution status were investigated.ResultsTotal of 61 patients constituted the study group. There were 40 female and 21 male patients with an average age of 43.8 ± 10.5 years (19‐67 years). Preoperatively, mean BMI, blood glucose levels and HbA1c were 48.8 ± 8.5 kg/m2, 133.6 ± 47.4 mg/dL and 7.4 ± 1.1, respectively. The mean blood glucose level at the postoperatively 5th day was 88.0 ± 16.3 mg/dL (median: 84 mg/dL) (P < .001). Fifty‐nine out of 61 patients improved their glycaemic control.ConclusionsIt is noteworthy that LSG can control blood glucose levels in short term after surgery regardless of weight loss. Therefore, LSG should be preferred at earlier stages in the treatment of obesity‐related T2DM in order to prevent T2DM‐related complications.