bluuebunny/crossref_metadata_2025_split huggingface dataset:
A requirement for Potassium and Calcium Channels during the Endosomal Trafficking of Polyomavirus Virions
Authors: Dobson Samuel J. , Mankouri Jamel , Whitehouse Adrian 
Published: October 2019

ABSTRACTFollowing internalisation viruses have to escape the endocytic pathway and deliver their genomes to initiate replication. Members of thePolyomaviridaetransit through the endolysosomal network and through the endoplasmic reticulum (ER), from which heavily degraded capsids escape into the cytoplasm prior to nuclear entry. Acidification of endosomes and ER entry are essential in the lifecycle of polyomaviruses, however many mechanistic requirements are yet to be elucidated. Alteration of endocytic pH relies upon the activity of ion channels. Using two polyomaviruses with differing capsid architecture, namely Simian virus 40 (SV40) and Merkel cell polyomavirus (MCPyV), we firstly describe methods to rapidly quantify infection using an IncuCyte ZOOM instrument, prior to investigating the role of K+and Ca2+channels during early stages of infection. Broad spectrum inhibitors identified that MCPyV, but not SV40, is sensitive to K+channel modulation. In contrast, both viruses are restricted by the broad spectrum Ca2+channel inhibitor verapamil, however specific targeting of transient or long lasting Ca2+channel subfamilies had no detrimental effect. Further investigation revealed that tetrandrine blockage of two-pore channels (TPCs), the activity of which is essential for endolysosomal-ER fusion, ablates infectivity of both MCPyV and SV40 by preventing disassembly of the capsid, which is required for the exposure of minor capsid protein nuclear signals necessary for nuclear transport. This study therefore identifies a novel target to restrict the entry of polyomaviruses.IMPORTANCEPolyomaviruses establish ubiquitous, asymptomatic infection in their host. However, in the immunocompromised these viruses can cause a range of potentially fatal diseases. Through the use of SV40 and MCPyV, two polyomaviruses with different capsid organisation, we have investigated the role of ion channels during infection. Here, we show that Ca2+channel activity is essential for both polyomaviruses and that MCPyV is also sensitive to K+channel blockage, highlighting new mechanistic requirements of ion channels during polyomavirus infection. In particular, tetrandrine blockage of endolysosomal-ER fusion is highlighted as an essential modulator of both SV40 and MCPyV. Given that the role of ion channels in disease have been well characterised, there is a large panel of clinically available therapeutics that could be repurposed to restrict persistent polyomavirus infection and may ultimately prevent polyomavirus-associated disease.
Genetic engineering of hoxb8 immortalized hematopoietic progenitors: a potent tool to study macrophage tissue migration
Authors: Accarias Solene , Sanchez Thibaut , Labrousse Arnaud , Ben-Neji Myriam , Boyance Aurélien , Poincloux Renaud , Maridonneau-Parini Isabelle , Le Cabec Véronique 
Published: October 2019

AbstractTumor-associated macrophages (TAM) are detrimental in most cancers. Controlling their recruitment is thus potentially therapeutic. We showed that TAM perform the protease-dependent mesenchymal migration in cancer, while macrophages perform amoeboid migration in other tissues. Inhibition of mesenchymal migration correlates with decreased TAM infiltration and tumor growth, providing rationale for a new cancer immunotherapy specifically targeting TAM motility. To identify new effectors of mesenchymal migration, we produced ER-Hoxb8-immortalized hematopoietic progenitors with unlimited proliferative ability. The functionality of macrophages differentiated from ER-Hoxb8 progenitors was compared to bone marrow-derived macrophages (BMDM). They polarized into M1- and M2-orientated macrophages, generated ROS, ingested particles, formed podosomes, degraded the extracellular matrix, adopted amoeboid and mesenchymal migration in 3D, and infiltrated tumor explantsex vivousing mesenchymal migration. We also used the CRISPR/Cas9 system to disrupt gene expression of a known effector of mesenchymal migration, WASP, to provide a proof of concept. We observed impaired podosome formation and mesenchymal migration capacity, thus recapitulating the phenotype of BMDM isolated fromWasp-KO mice. Thus, we validate the use of Hoxb8-macrophages as a potent tool to investigate macrophage functionalities.Summary statementWe validate the use of ER-Hoxb8-immortalized hematopoietic progenitors combined to CRISPR/Cas9 technology as a potent tool to investigate macrophage functionalities with a large scale of applications.
Upgrade of laser pump time-resolved X-ray probes in Beijing synchrotron
Authors: Yu Can , Zhang Yan , Lei Ge , Wang Hao , Wang Yu-hang , Zhang Bing-Bing , Sun Da-Rui , Zhao Jia-Li , Zhou Yang-Fan , Li Qiu-Ju , Gao Zhen-hua , Zhan Fei , Tao Ye 
Published: October 2019

The upgrade of the laser pump time-resolved X-ray probes, namely time-resolved X-ray absorption spectroscopy (TR-XAS) and X-ray diffraction (TR-XRD), implemented at the Beijing Synchrotron Radiation Facility, is described. The improvements include a superbunch fill, a high-efficiency fluorescence collection, an efficient spatial overlap protocol and a new data-acquisition scheme. After upgrade, the adequate TR-XAS signal is now obtained in a 0.3 mM solution, compared with a 6 mM solution in our previous report. Furthermore, to extend application in photophysics, the TR-XAS probe is applied on SrCoO2.5 thin film. And for the first time, TR-XAS is combined with TR-XRD to simultaneously detect the kinetic trace of structural changes in thin film.
Study on unbiased interval grey number prediction model with new information priority
Authors: Li Ye , Li Juan 
Published: October 2019

Purpose
The purpose of this paper is to construct an unbiased interval grey number prediction model with new information priority for dealing with the jumping errors from difference equation to the differential equation in the prediction model of interval grey number.

Design/methodology/approach
First, this study obtains a set of linear equations about the model parameters by taking the minimum error sum of squares between the accumulative sequence and its simulation values as criterion, and solves them on the basis of the Crammer rule. Then, according to the new information priority principle, it selects the last number of the accumulated generation sequence as the initial value and gives the expression of the time response function by the recursive iteration method to establish the interval grey number prediction model.

Findings
This paper provides an unbiased interval grey number prediction model with new information priority, and the example analysis shows that the method proposed in this paper has higher prediction precision and practicality.

Research limitations/implications
If there is a better method to whiten the interval grey number, so as to fully tap the grey information contained in it, the accuracy of the model will be higher.

Practical implications
The model proposed in this paper can avoid the error caused by jumping from difference equation to differential equation and make full use of new information. It can be better used in a problem where new information has a great influence on prediction results.

Originality/value
This paper selects the last number of the accumulated generation sequence as the initial value and gives the expression of the time response function by the recursive iteration method. Then, it constructs an unbiased interval grey number prediction model with new information priority.
Technology integration for students’ information and digital literacy education in academic libraries
Authors: Rafi Muhammad , JianMing Zheng , Ahmad Khurshid 
Published: October 2019

PurposeDigital literacy has become a widely studied topic for connecting people, participation in government and public affairs. It encourages library professionals to train students and effectively acquire, preserve and disseminate knowledge. Academic libraries involve using digital technology improved the students’ level of digital literacy and technological skills. This paper aims to understand the students’ technological competence in using database resources and searching for online information. In addition, to evaluate the training contents and designated instructors on digital literacy education, the study also focuses on digitally exposed students’ learning behavior, the library management and students’ academic interaction on new technological trends.Design/methodology/approachThis study uses a quantitative method for which primary data were collected from university students using a structured research questionnaire. Through targeted random sampling techniques, 1,500 printed questionnaires were distributed among students, and 1,266 (84.4%) responses were received. The authors applied descriptive statistics to analyze the demographic information of male and female students. The statistical technique Chi-square test (χ2) was applied using SPSS to determine the association between variables. Moreover, Bonferroni correction for the new a value [modified = 0.05/number of possible comparisons/analysis for each question shown in Tables (II-IX)] was also used to avoid a family-wise error.FindingsThe results inferred a strong association between students’ technological skill with using digital tools, utilization of database resources and browsing networked information on the Web. The association between universities in terms of recruiting designated instructors on digital literacy education, introducing ICT based curriculum and the association between library management in maintaining a regular training program on digital literacy demonstrated a negative association. Besides, the findings disclosed that student’s digital exposure is not the forcing factor in developing technological competence, but found to be a more encouraging factor. It was also concluded that library technological involvement significantly improves student technology-based learning skills.Practical implicationsThe results obtained after the analyzed data may help library management in providing long-term strategy for improving digital literacy education and training for students in the effective utilization of technology for better academic performance and social integration.Originality/valueThe study was based on quantitative methods using a questionnaire. Extensive database coverage and digital literacy skills based on coach input can improve student performance and academic quality.
Understanding second grader’s computational thinking skills in robotics through their individual traits
Authors: Baek Youngkyun , Yang Dazhi , Fan Yibo 
Published: October 2019

Purpose
This study aims to investigate the relationship between the personal traits and computational thinking skills of second graders within the context of robotics activities.

Design/methodology/approach
Through literature review, a research model and hypotheses were tested with 122 second graders after robotic activities.

Findings
The hypothesized model showed that learning preference, intrinsic motivation and self-efficacy were the main predictors of coding achievement and computational thinking skills, while no direct relationship was found between learning preference, intrinsic or extrinsic motivation. The final path analysis revealed that intrinsic and extrinsic motivation predict self-efficacy, self-efficacy predicts coding achievement and coding achievement predicts computational thinking skills. Another important finding was the strong impact of self-efficacy on coding achievement, as well as computational thinking skills. Results are interpreted with reference to implications for potential methods of improving computational thinking skills when using robotics in the lower grades in elementary schools.

Research limitations/implications
This study not only examined these relationships but also proposed, tested and built a research model containing a wide range of personal traits based on path analysis and multiple regression analysis, which, to the best of the researchers’ knowledge, has not been investigated in the current literature.

Practical implications
As reflected in the final research model, self-efficacy played an important role in impacting second grader’s coding achievement and computational thinking skills.

Originality/value
Few studies have investigated the various relationships in the context of robotics instruction in elementary schools as in this study. Given the increasing popularity of robotics education in elementary schools, the re-examination and identification of the pivotal role of self-efficacy in predicting second graders’ learning of coding and computational thinking skills have important implications for the implementation of robotics education.
Conservation management plans
Authors: Worthing Derek , Organ Samantha 
Published: October 2019

Purpose
The purpose of this paper is to identify factors that contribute to the development of an effective conservation management plan (CMP).

Design/methodology/approach
The approach was based on a literature review from which key issues and concerns were identified. This was followed by in-depth interviews with a number of creators and users of CMPs.

Findings
CMPs have developed as an identifiable process with the key stages having a logic and synergy with each other. The research found that undue emphasis was placed on some stages at the expense of others which lead to ineffective management tools often being produced. The reasons for this are related to the interests and background of the creators and a lack of interaction with organisational culture and processes – and importantly a failure to engage with frontline staff. In addition, there were also resource and skill constraints within the client organisation.

Research limitations/implications
Interviews were conducted with six creators (consultants) and seven users. These were mostly from national heritage organisations and specialist heritage consultants. A wider range of user organisations and consultants could be identified for follow-up research. Also those who actually deliver CMPs “on the ground” and day to day could form an important part of the development of this research.

Practical implications
CMPs should be practical working management tools which have to be used by the heritage organisation in order to be effective. This research will hopefully help practitioners focus on what needs to be done in order to produce an effective plan.

Social implications
The conservation of built heritage is essentially concerned with the protection of a social good. CMPs have the potential to provide effective protection of that which is seen as valuable and significant to individuals, groups and society at large.

Originality/value
The management of heritage is an area that is generally under-researched. This work will hopefully be engaged with by academics and practitioners in order to help establish and promote a wider interest in the field.
The power of place in public leadership research and development
Authors: Jackson Brad 
Published: October 2019

PurposeThe purpose of this paper is to foreground place as a critical and central concern for public leadership research, development and practice.Design/methodology/approachThis invited essay draws on the author’s own research and development work engaging in collaborative place-based interventions with academics, policy makers and practitioners.FindingsPlace is one of six heuristic lenses in a Leadership Hexad that has been developed to interrogate and better understand leadership in a multi-dimensional manner. Place can provide an important theoretical and practical fulcrum for bridging both collaborative governance and collective leadership and public and political leadership as well as facilitating cross-sectoral leadership.Practical implicationsThis essay argues that more time and effort should be invested into researching and developing place leadership to complement the already extensive efforts to promote collaborative governance and place-based policy initiatives. Place leadership development should be genuinely cross-sectoral in its ambition and should focus on developing emerging and established leaders from the public, private, not-for-profit and indigenous sectors to tackle place-based problems and opportunities.Originality/valueThis essay draws on experience undertaking academic research and conducting leadership development that draws from and feeds into policy and practice. It utilises research from geography, leadership studies, public management, public policy and political science to gain a more sophisticated understanding of the relationship between place and public leadership and how this can be harnessed to improve economic and social impact.
Can family characteristics influence the future labor situation of children? Evidence for Spain
Authors: Morales Marina 
Published: October 2019

Purpose
The purpose of this paper is to analyze whether family support, measured through the labor status of parents and the presence of both parents in the household during adolescence, may be an important determinant of unemployment in Spain.

Design/methodology/approach
To address this issue, we follow the Quantity-Quality model of Becker–Lewis (Becker and Lewis, 1973), using data from the Survey of Living Conditions (2011).

Findings
First, the results show that individuals living with both parents at home during their teenage years are less likely to be unemployed in the future. Second, the authors find evidence of the intergenerational transmission of unemployment outcomes, and that the unemployment status of the mother is strongly transferred to the child. Additionally, the authors extend this work to an analysis of other labor characteristics, finding that family support is an important determinant of self-employment and temporary employment. The findings are robust to controls for observable and unobservable characteristics by region, and to the use of different subsamples.

Originality/value
This study can be considered as first evidence of the effect of family support during adolescence on the Spanish labor market.
Do remittances promote human development? Empirical evidence from developing countries
Authors: Huay Chong Siew , Winterton Jonathan , Bani Yasmin , Matemilola Bolaji Tunde 
Published: October 2019

Purpose
The purpose of this paper is to analyse the impact of remittances on human development in developing countries using panel data from 1980 to 2014 and to address the critical question of whether the increasing trend of remittances has any impact on human development in a broad range of developing countries.

Design/methodology/approach
Usual panel estimates, such as pooled OLS, fixed or random effects model, possess specification issues such as endogeneity, heterogeneity and measurement errors. In this paper, we, therefore, apply dynamic panel estimates – System generalised method of moment (Sys-GMM) developed by Arellano and Bond (1991) and Arellano and Bover (1995). This estimator is able to control for the endogeneity of all the explanatory variables, account for unobserved country-specific effects that cannot be done using country dummies due to the dynamic structure of the model (Azman-Saini et al., 2010).

Findings
The effect of remittances is statistically significant with positive coefficients in developing countries. The significant coefficient of remittances means that, holding other variables constant, a rise in remittance inflows is associated with improvements in human development. A 10 per cent increase in remittances will lead to an increase of approximately 0.016 per cent in human development. These findings are consistent with Üstubuci and Irdam (2012) and Adenutsi (2010), who found evidence that remittances are positively correlated with human development.

Practical implications
The paper considers implications for policymakers to justify the need for more effective approaches. Policymakers need to consider indicators of human development and to devise public policies that promote income, health and education, to enhance human development.

Originality/value
The question of whether remittances affect human development has rarely been subject to systematic empirical study. Extant research does not resolve the endogeneity problem, whereas the present study provides empirical evidence by utilising dynamic panel estimators such as Sys-GMM to tackle the specification issues of endogeneity, measurement errors and heterogeneity. The present study provides a benchmark for future research on the effect of remittances on human development.
Creativity, innovation and smartness in destination branding
Authors: Trinchini Lino , Kolodii Natalia Andreevna , Goncharova Natalia Aleksandrovna , Baggio Rodolfo 
Published: October 2019

PurposeThe purpose of this paper is to examine the role of creativity and innovation as important attributes of smartness in cities/destination branding.Design/methodology/approachA conceptual support to the notion of smart destination branding is provided by discussing the relationship between creativity, innovation and technology as determinants for the smartness concept applied to destination branding and marketing. This paper adopts a qualitative and logical-deductive approach. The cases of Milan (Italy) and Tomsk (Russia) are presented and compared as smart cities approach to branding within and outside Europe. The authors emphasise the importance of smart destination branding strategies based on people participation, creativity and innovation as drivers of smart urban development.FindingsThe endogenous ability of cities/destinations to embrace creativity across stakeholders is essential to smart branding strategies relying on advanced information and communication technologies. The entwined connection between smart cities/destinations creative initiatives and innovation underpins innovative branding strategies.Research limitations/implicationsThe paper is conceptual and the findings cannot be generalised to other destinations, even if a couple of examples are briefly discussed. The authors intend to provide a basis for future research concerning smart destination branding.Originality/valueThe technological, human and institutional dimensions of smart cities and smart tourism destinations have been increasingly addressed by scholars and practitioners. Despite the reference and attention to human factors is not new, there is still a lack of extensive focus on creativity as crucial driver of innovation in smart destination branding. This paper aims to fill such gap by focussing on the implications of urban smartness driven by creativity and innovation in destination branding and marketing.