bluuebunny/crossref_metadata_2025_split huggingface dataset:
205 Effect of supplemental trace mineral source on performance, health, and carcass characteristics in finishing beef steers
Authors: Heldt Jeff , Holland Ben , Word Alyssa , Karr Kendall 
Published: November 2020

Abstract
Crossbred beef steers (n = 2,420; 357 ± 16 kg BW) were used to evaluate the effects of supplemental trace mineral sources on the performance and carcass characteristics on feedlot cattle. Steers were housed in 20 pens (120–125 steers per pen) with 10 pens per treatment. Supplemental trace mineral treatments (100% DM) consisted of: control: 8.3 mg/kg of Cu (100% CuSO4), 83.4 mg/kg of Zn (64.1% ZnSO4/35.9% Zn amino acid complex), and 28.7 mg/kg of Mn (100% MnSO4); hydroxy: 8.3 mg/kg of Cu (100% basic Cu chloride), 82.9 mg/kg of Zn (100% Zn hydroxychloride), and 19.3 mg/kg of Mn (100% Mn hydroxychloride). Steers were fed trace mineral treatments for 158 d and harvested at a commercial abattoir. Data were analyzed as a randomized complete block design with pen as the experimental unit. There were no differences between treatments in DMI, ADG, feed:gain, final BW, or mortality (P ≥ 0.38); however, morbidity tended (P = 0.06) to be greater for hydroxy than control (3.44 or 2.20%, respectively). Hot carcass weight, dressing percentage, marbling score, and longissimus muscle area were unaffected (P ≥ 0.28) by treatment, though backfat tended (P = 0.07) to be greater for hydroxy compared to control (1.55 or 1.51 cm, respectively). Distributions of quality grades were not different (P ≥ 0.14) with the exception of increased (P = 0.01) Prime carcasses in control compared to hydroxy (2.57 or 1.18%, respectively). Distributions of yield grades 2, 3, and 4 were not different (P ≥ 0.17) between treatments, though percentage of carcasses assigned 1 was reduced (P = 0.01) and 5 was increased (P = 0.05) in hydroxy compared to control. These data indicate steers fed 100% hydroxychloride trace minerals perform similiarly to steers fed CuSO4, MnSO4, and a ZnSO4/Zn amino acid complex combination.
222 Partitioning of retained energy between protein and fat gain, and heat of product formation and support metabolism in growing beef cattle
Authors: Lancaster Phillip A 
Published: November 2020

Abstract
Multiple linear regression inaccurately computes the efficiency of energy use for protein and fat gain. The objective was to quantify efficiency of metabolizable energy use for protein and fat gain along with heats of product formation and support metabolism. A literature search was performed to compile data (31 studies, 214 treatment means) on metabolizable energy intake (MEI) and composition of empty body gain in growing steers and heifers. Data analyses were performed using R statistical package for mixed models with study as random variable. Linear regression of MEI on energy gain (EG; P &lt; 0.001; R2 = 0.627) resulted in an estimate of metabolizable energy for maintenance (MEm) of 156 kcal/kg.75 and efficiency of ME use for gain of 0.518. Linear regression of MEI on EG as protein and fat (P &lt; 0.001; R2 = 0.623) resulted in an estimate of MEm of 149 kcal/kg.75, and efficiency of protein (kp) and fat (kf) gain of 0.274 and 0.585, respectively, resulting in an overall efficiency of EG of 0.520. Nonlinear regression model (EG = kg*(MEI-MEm)) resulted in an estimate of MEm of 103 kcal/kg.75 and efficiency of EG of 0.342. The heat of product formation was assumed to be 0.48 (1 – 0.52) and the heat of support metabolism (HiEv) 0.18 (0.52 – 0.34). Multivariate regression was used to fit simultaneous models for EG as protein (EGp = (kp+HiEvp)*k*MEA) and fat (EGf = (kf+(0.18-HiEvp))*(1-k)*MEA). Estimates (P &lt; 0.001) of kp and kf were 0.12 ± 0.01 and 0.63 ± 0.02, and HiEvp and proportion of ME available for protein gain (k) were 0.11 ± 0.01 and 0.75 ± 0.01, respectively. The heat of product formation and support metabolism for protein were 0.77 and 0.11, and fat were 0.30 and 0.07, respectively. In conclusion, efficiency of ME use for protein was lesser than for fat gain, and heat of support metabolism was greater for protein than fat gain.
213 Feeding essential oils and α-amylase or its association with 25-hydroxy-vitamin-D3 improves productive performance by feedlot cattle
Authors: Niehues Maria Betania , Perdigão Alexandre , Valério de Carvalho Victor V , Acedo Tiago S , Vasconcellos Guilherme S F M , Tamassia Luis Fernando , Martins Cyntia L , Millen Danilo D , Arrigoni Mário B 
Published: November 2020

Abstract
Our objective was to evaluate the effects of associating feed additives on performance of finishing cattle when fed a high-concentrate diet for 105 days. Twenty-four Angus-Nellore crossbred bulls (iBW, 456 ± 10.04 kg; age, 18 mo) were randomly allocated to three treatments with eight replicates per treatment (animal as experimental unit). The treatments were: 1) Control (MON) - Sodium Monensin, 26 mg/kg DM; 2) Crina® RumistarTM (CR) - a blend of essential oils, 90 mg/kg DM + exogenous α-amylase, 560 mg/kg DM) and 3) CR + HyD® (25-hydroxy-vitamin-D3 at 1 mg/animal/d). The DM intake and animalsˈ weight variables were assessed individually, by using the Intergado® electronic system and the Bosch® Precision Livestock platform placed in the feedlot pen, respectively. Data were analyzed using the Mixed procedure of SAS and means comparison evaluated by Tukey test at P &lt; 0.05. The initial BW was used as a covariate when significant. Feeding CR and CR+HyD increased DMI (13.18 and 12.82 kg vs. 10.77 kg P &lt; 0.01) and tended to increase ADG (1.94, 1.92 vs. 1.68kg/d, P = 0.07) and final BW (654, 652 vs. 628 kg, P = 0.08) compared with MON. Likewise, animals fed CR and CR+HyD had greater carcass ADG (1.38, 1.41 vs. 1.14 kg/d, P &lt; 0.01), and HCW (368.53 and 371.87 vs. 344.13 kg; P &lt; 0.01) compared to MON. In addition, feeding CR+HyD increased the dressing percentage in 2.1 percent points compared with MON (57.4 vs. 55.3%; P &lt; 0.03). The G:F and the biological efficiency were similar among treatments (P = 0.50). We conclude that including Crina® RumistarTM +HyD® can be used as a tool to increase carcass production by feedlot cattle.
390 Micronutrients, One-Carbon Metabolism, and Epigenetics: Potential Developmental and Production Outcomes
Authors: Crouse Matthew S , Caton Joel , Ward Alison K 
Published: November 2020

Abstract
One-carbon metabolism is the network of biochemical pathways in which methyl groups are transferred from one compound to another for methylation processes. Expansion of the core pathway connects one-carbon metabolism to polyamine synthesis, nucleotide synthesis, redox metabolism, and the citric acid cycle. One-carbon metabolites (OCM) are methyl donors and cofactors which play key roles in the one-carbon metabolism pathway and include B-vitamins (choline, vitamin B12, vitamin B6, riboflavin, and folate), minerals (cobalt and sulfur) and amino acids (methionine, serine, and glycine). One-carbon metabolites are fundamental methyl donors for epigenetic modifications. Immediately post-fertilization, the embryonic genome undergoes epigenetic remodeling, and is the time when cell division is greatest (cell divisions/total cell number). At this time OCM supplementation may have its greatest impact on programming of offspring development, growth and postnatal performance, due to established metabolic roles in epigenetics (methyl transfer), growth (polyamine and nucleotide synthesis), and energetics. Limited data are available, however, which directly investigates the developmental effects of OCM supplementation in ruminants. In dairy cows, OCM supplementation in late gestation increased calf birth weight, nutrient sensing pathway activation, and offspring performance through the pre-weaning period. Methyl deficient diets during the pre-conception period in ewes altered the offspring hepatic methylome at 90 days of gestation, as well as the body composition and insulin tolerance of ram lambs at 22-mo of age. Bovine embryonic fibroblasts cultured in vitro with increasing OCM had greater growth rates and mitochondrial respiration parameters. Additional research into the area of one-carbon metabolism and the roles that OCM supplementation may play on postnatal function will provide new knowledge that could lead to altered management practices and increased efficiency of beef cattle. USDA is an equal opportunity provider and employer.
398 Towards new feeding approaches for optimizing nutritional status, immunity and host-microbiota interactions in neonatal and weaned piglets
Authors: Lessard Martin , Blais Mylène , Talbot Guylaine , Matte J Jacques , Letellier Ann , Thibodeau Alexandre , Guay Frédéric 
Published: November 2020

Abstract
Lactation, feeding conditions, microbial interventions and piglet growth in the first few weeks of life have important impact on the intestinal microbiota establishment and immune system development of piglets. Indeed, colostrum and milk contain various bioactive components such as immune factors, antimicrobial peptides and oligosaccharides that contribute to maintain intestinal homeostasis and regulate interactions between microbiota and host immune system. Recent results revealed that low birth weight piglet (LBWP) with poor weight gain during the first two weeks of life develop different intestinal microbiota and immune response profiles compared to high BWP (HBWP) littermates. Consequently, piglets within litters may have different resilience to infections after weaning and benefit from feed additives in a specific manner. A study has been performed to evaluate the potential of bovine colostrum extract (BC) as replacement to plasma proteins for improving gut health and resilience to Salmonella infection in piglets. Results revealed that in weaned piglets fed BC, intestinal microbiota was differently modulated and bacterial dysbiosis induced by Salmonella was restored faster. Moreover, expression of genes involved in innate immunity such as β-defensin-2 and glutathione peroxidase-2 was respectively down- and up-regulated in BC fed piglets. A combination of dietary supplementation with BC, cupper and vitamins A and D has also been tested in LBWP and HBWP, and there is clear evidence that BC in combination with other feed additives promote growth and gut health in both LBWP and HBWP. The porcine intestinal epithelial cell line IPEC-J2 was used to better understand the functional properties of BC. Results indicated that BC improves wound healing, enhances barrier function and modulates the expression of several genes involved in innate immune response. Finally, as microbial intervention, the potential of fecal transplantation to modulate intestinal microbiota and immune system development of piglets is under investigation and will be discussed.
291 Influence of feeding amino acid complexes of zinc and copper on growth performance and carcass response of finishing steers fed ractopamine hydrochloride
Authors: Engel Chanda , Tibbetts Gary , Branine Mark 
Published: November 2020

Abstract
A 42-d research study evaluated effects of feeding beef steers increasing levels of dietary Zn from Zn methionine (AAC-Zn; ZINPRO, Zinpro Corporation, Eden Prairie, MN) with or without supplemental Cu from Cu amino acid complex (AAC-Cu; Availa Cu, Zinpro Corp.) concurrently with ractopamine hydrochloride (RAC; Optaflexx, Elanco Animal Health, Greenfield, IN). One hundred-twenty steers (mean BW = 624 kg), ≤ 50 d from projected harvest were randomized to three pens (40 steers/pen) equipped with GrowSafe Systems (Calgary AB, Canada) feed bunk technology. Each pen was assigned to one of three treatment diets: 1) basal finishing diet + 30 mg Zn/kg DM from AAC-Zn (CON); 2) basal finishing diet + 90 mg Zn/kg DM from AAC-Zn (AAC-Zn90); or 3) AAC-Zn90 diet + 10 mg Cu/kg DM from AAC-Cu (AAC-Zn/Cu). Following allocation to treatments, cattle were acclimated to pen cohorts and GrowSafe feed bunks for 7 days. All steers were fed 300 mg RAC∙hd-1∙d-1 starting 35 d prior to harvest. Individual feed intake measurements began with RAC feeding and continued for 35 d until cattle were shipped for harvest. Carcass data were collected from each steer. Data were analyzed with individual animal as the experimental unit using PROC MIXED and PROC GLIMMIX procedures of SAS 9.4 (SAS Institute, Cary, NC). Numerically AAC-Zn90 fed cattle had heavier carcass weights than CON and AAC-Zn/Cu. Steers fed AAC-Zn90 had greater (P = 0.02) marbling scores compared to AAC-Zn/Cu. Steers fed AAC-Zn90 had lower backfat thickness (P = 0.02) and numerically greater marbling scores compared to CON. A biphasic program of feeding a lower level of AAC-Zn for the duration of the finishing phase followed by an increased rate of AAC-Zn during RAC feeding may optimize overall live and carcass response and improve total individual animal value.
PSXII-24 Identification of selection signatures for response of American mink to Aleutian mink disease virus infection
Authors: Karimi Karim , Farid A Hossain , Myles Sean , Miar Younes 
Published: November 2020

Abstract
Aleutian disease (AD) is one of the most important health problems in the mink industry worldwide, leading to economic losses. We used a set of single nucleotide polymorphisms (SNPs) to detect the genomic regions potentially under selection for response to Aleutian mink disease virus (AMDV) infection in black American mink. A total of 191 mink which were inoculated with a local strain of AMDV and survived until pelting were genotyped using genotyping-by-sequencing technique. The presence of viral DNA in the spleen samples were tested by polymerase chain reaction. After filtering, 47,800 SNPs at 171 individuals were used for further analyses. Signatures of selection for response to AMDV infection were detected using fixation index (FST) and nucleotide diversity (θπ) statistics measured between negative and positive groups. The overlap of top 1% SNPs obtained from both FST and θπ scores were considered as potential selection signs. This measurement identified a total of 21 candidate regions containing 11 genes which were likely subjected to selection for viral clearance. Several identified genes were those that modulate immune system (TCF4), reproductive process (CATSPERB, MAS1 and IGF2R), response to stimulus (WNT11 and MAS1), and functions of heart (TENM4 and WNT11) and liver (IGF2R). In addition, gene ontology showed that 63.6% of detected genes (seven) were involved in binding activities (GO:0005488). These genes can be used in molecular assessment of viral clearance in American mink. The results indicated that selection for viral clearance and thus AD tolerant animals can be a feasible strategy to deal with the global AMDV infection on mink farms.
PSVII-23 Using low-moisture molasses-based blocks to supplement Ca salts of soybean oil to forage-fed beef cows
Authors: Brandão Alice , Cooke Reinaldo F , Schubach Kelsey , Colombo Eduardo , Scatolin Giovanna , Rett Bruna , Pohler Ky G 
Published: November 2020

Abstract
This experiment compared plasma fatty acid (FA) profile of forage-fed beef cows receiving a molasses-based supplement enriched with Ca salts of soybean oil [(CSSO); 24.7% of dry matter (DM)] via a self-fed low-moisture block (LMB) or hand-fed granular concentrate daily (CONC). Thirty-six cows were allocated to 1 of 9 drylot pens, which were enrolled in a replicated 3 x 2 Latin square design with 2 periods of 42 d. Pens were randomly assigned to receive 1 of the 3 treatments, in a manner that pens did not receive the same treatment in both periods. Cows received hay for ad libitum consumption during the study. Hay intake was recorded daily from d 0 to 42, and LMB intake was recorded from d 14 to 42. The CONC was offered at 0.420 kg/cow daily (DM basis) from d 0 to 13, and then adjusted (d 14 to 42) to match LMB intake. Average LMB intake during the initial 13 d was 0.846 ± 0.107 kg/cow daily (DM basis). Supplement DM intake did not differ (P = 0.39) between LMB and CONC cows from d 14 to 42. No treatments effects were noted (P ≥ 0.40) for hay intake, BCS, and BW. Plasma FA profile did not differ (P ≥ 0.20) between treatments on d 0. From d 14 to 42, plasma concentrations of linoleic acid, ω-6 polyunsaturated FA, and total FA were greater (P &lt; 0.01) in CONC and LMB vs. NOSUPP cows. Plasma concentrations of these FA were also greater (P ≤ 0.03) in LMB vs. CONC cows on d 14, but did not differ (P ≥ 0.35) on d 28 and 42. Inclusion of CSSO into LMB resulted in similar incorporation of ω-6 polyunsaturated and total FA in the circulation compared with CONC offered at the same daily rate.
PSVII-12 Investigating the influence of copper supplementation on copper homeostatic genes in bovine liver
Authors: Tillquist Nicole M , Thorndyke Meghan P , Thomas Tyler A , Coleman Stephen J , Engle Terry E 
Published: November 2020

Abstract
The objective of this experiment was to investigate the influence of copper (Cu) supplementation on Cu homeostatic genes in bovine liver. Liver samples were obtained from multiparous cows (n = 3 cows per treatment) from a previous experiment. Treatments consisted of: 1) no supplemental Cu (-Cu; total diet contained 7.0 mg Cu/kg DM) and 2) 3 mg Cu/kg DM (+Cu; total diet contained 10.0 mg Cu/kg DM). Cows were fed their respective diets for 420 d. Liver samples were harvested immediately upon slaughter, rinsed with phosphate-buffered saline solution, diced into small pieces, and placed into RNAlater and frozen at -80°C. Total RNA was extracted from all samples, and quantitative RT-PCR was used to determine the abundance of transcripts for proteins involved in Cu homeostasis in liver tissue. The target transcripts were: ALDH2, APOA1, ATOX1, ATP7A, ATP7B, BHMT, BLVRB, CA2, CCS, COX17, CTR1, ELN, GAPDH, GLUD1, GSS, LOXL1, PDIA3, SOD1, SOD3. The relative abundance of APOA1, ATOX1, ATP7B, BLVRB, CTR1, GLUD1, LOXL1, and SOD3 mRNA was greater (P &lt; 0.05) in cows receiving supplemental Cu when compared to cattle not receiving supplemental Cu. These data indicate possible differences in Cu homeostasis in the liver based on Cu supplementation. Further investigation is warranted to determine if a difference in the relative abundance of identified transcripts are related to differences in associated protein production and function.
PSXI-33 Effects of weight loss and feeding a high-protein, high-fiber diet on blood metabolite profiles, body composition and voluntary physical activity in overweight dogs
Authors: Phungviwatnikul Thunyaporn , Belchik Sara E , Swanson Kelly S 
Published: November 2020

Abstract
Canine obesity can be managed by dietary energy restriction using a specifically formulated weight loss diet. The objective of this study was to determine the effects of weight loss on body composition, voluntary physical activity, and blood metabolites of overweight dogs while being fed a high-protein, high-fiber diet. All procedures were approved by the University of Illinois Institutional Animal Care and Use Committee prior to experimentation. Twelve overweight adult spayed female dogs (BW: 15.3±2.1 kg, BCS: 8.1±0.6) were fed a high-protein (CP: 42.0% DMB), high-fiber (TDF: 22.0% DMB) diet during a 5-wk baseline phase (wk 0) to identify food intake needed to maintain BW. A 24-wk weight loss phase followed. After wk 0, food was initially provided at 80% the amount needed to maintain BW and then adjusted weekly with a goal of 1.5–2% weight loss per wk. Data were analyzed statistically overtime using SAS 9.4. After 24 wk, dogs lost 31.2% of initial BW (P &lt; 0.0001), with 1.4±0.7% weight loss per wk. BCS decreased by 2.8 units (P &lt; 0.0001). During weight loss, dogs consumed an average of 457.5±61.4 kcal/d, with energy intake being reduced by a total of 43.8% by wk 24 compared to baseline. Lean muscle mass, fat mass, and fat percentage were reduced (P &lt; 0.0001) by 1.3 kg, 3.1 kg, and 11.7% respectively. Serum triglycerides, alkaline phosphatase, white blood cell counts, and neutrophils were decreased (P &lt; 0.0001), but serum bilirubin, creatinine, and blood urea nitrogen were increased (P &lt; 0.01) over time. Average daily physical activity changed over time, but was not greatly different due to weight loss. Our results suggest that a high-protein, high-fiber diet promotes fat mass loss, minimizes lean muscle mass loss, and reduces inflammatory marker and triglyceride concentrations in overweight dogs. Therefore, it is a suitable nutritional solution for weight loss programs in dogs.
PSIX-36 Animal welfare and its science in Nigeria;past and present outlook
Authors: Alabi Olufemi M , Akinoso Sabainah O , Alabi Banwo O 
Published: November 2020

Abstract
Animal welfare is becoming an interesting global issue which must be given the best of attention by researchers, producers, consumers and the governments. The need to ensure the general well-being of the wild and domestic animals in every aspect of their husbandry is mandatory now in many developed countries of the world, and poultry industries are responding positively to this new dimension of husbandry. Species of animal are sensitive to the way they are being managed in terms of housing and other handlings. Meanwhile, there is little or no concern on subjects of of applied ethology in developing areas of the world such as Africa and Asia, and particular in countries like Nigeria, where livestock production and wildlife conservation is currently experiencing tremendous growth. Unlike developing countries, issue of animal welfare are being promoted by researches, sponsored information, legislation and enforcement by relevant agencies in developed nations of the world. Factors such as poor economic and technological advancements, political instabilities, social insecurities, low level of awareness and lack of information on legal provisions on animal on animal welfare have been identified to be responsible for poor animal welfare and its science in developing countries. Despite the fact that livestock sector carries the highest percentage among agricultural facets commercially in Nigeria, the level of welfare of the domestic animals and those in the wild is still below standard. This paper is however suggesting that regardless of the level of economic development in most of the low and middle-income nations, researchers can be motivated into animal welfare science, the curricula of colleges and universities be expanded to include subjects of applied ethology, professional bodies and associations on animal welfare can be formed with international affiliations. All these may change the orientation of the people and governments in developing countries positively towards animal welfare.