bluuebunny/crossref_metadata_2025_split huggingface dataset:
Análisis demográfico para el manejo reproductivo de jaguares (Panthera onca) cautivos en parques zoológicos de Colombia
Authors: Jiménez González Santiago , Monsalve Redwan Haydy , Moreno Duarte María Angélica , Jiménez Escobar Claudia 
Published: January 2020

Se caracterizó demográfica y reproductivamente la población cautiva del jaguar (Panthera onca) (n=38) en zoológicos colombianos. Se analizaron registros (1970-2010), en los que se completaron datos de paternidad y maternidad mediante análisis de microsatélites de ADN. Se obtuvieron el censo, la distribución por sexo y edad, las tasas de fertilidad, supervivencia y mortalidad, la expectativa de vida media, el valor reproductivo y proyecciones reproductivas para 5, 10, 15, 20 y 100 años, conservando el 90 % de la diversidad genética. La población analizada en el 2010 estaba decreciendo (λ= 0.9876), el tiempo generacional (T) fue mayor para las hembras (T=18.21 años) que para los machos (T= 17.46 años) y la diversidad genética estuvo en ascenso entre 1994-2002 y se mantuvo estable entre 2002-2009 (GD=0.78). El análisis genético permitió estructurar la población cautiva, optimizando el análisis y la confiabilidad de los resultados, abriendo la posibilidad de aplicar estos métodos como modelo de conservación en otras especies amenazadas.
Ampliación del área de distribución de Micrurus camilae (Serpentes: Elapidae) en el Caribe colombiano
Authors: Pelaez Plazas Sergio Alejandro , Perlaza Berrio Lilia Alejandra 
Published: January 2020

Se presenta el primer registro de la especie Micrurus camilae para el departamento de Sucre, Colombia, ampliando su área de distribución y contribuyendo con datos sobre su hábitat. El individuo se encontró en un parche de vegetación en bosque seco tropical, a diferencia de los reportes anteriores donde se localiza en bosque húmedo tropical.
Mathematical modeling and comparative analysis of the Ebola epidemic reveal effective control measures
Authors: Author) Luosheng Wen(Former Corresponding , Li Junlong , Song Caiping , Yang Jingya , Zheng Jingmin , Zhou Lina , Zhang Zhujun , Pu Jie , Luo Yang , Zhang Hongyan , Wang Yungui , Author) Hao Wu(New Corresponding 
Published: January 2020

Abstract
Background
The Ebola virus disease (EVD) is a highly contagious disease which is caused by the Ebola virus. Various measures were used to prevent and control the spread of EVD in Guinea, Liberia Sierra and Leone. The aim of this study was to find out the most critical measures to prevent and control the spread of EVD and the effect of control in the three countries.
Methods
We used a novel deterministic compartmental model with the parameters that describes the spreading and control of Ebola, and then the data of confirmed case from the WHO was fitted to obtain the effect estimation of control measures. On the other hand, a comparative analysis also was used to explore the process.
Results
The results of our compartmental model showed that the control parameters ( a , c ) of pre-infection and post-infection are (0.1827, 0.1091), (0.5832, 0.3278) and (0.3696, 0.1281) in Guinea, Liberia Sierra and Leone, respectively. This means that the control measures in Liberia Sierra works best and that in Guinea works worst. The results of comparative analysis based on the same data showed that the quarantined individuals before infection ( R -squared R 2 = 0.848, standardized coefficients ε = 1.025), the safe burial teams ( R 2 = 0.772, ε = 0.365), and the Ebola treatment units (ETU) beds ( R 2 = 0.690, ε = 0.424) could significantly influence the incidence of EVD.
Conclusion
These findings indicted that a timely and effective quarantine plays a significant role in preventing and controlling the spread of EVD, and the findings would help us prevent and control the epidemic outbreak of new infectious disease in the future.
Physiological and transcriptomic analyses reveal the mechanisms underlying the salt tolerance of Zoysia japonica Steud.
Authors: Wang Jingjing , An Cong , Guo Hailin , Yang Xiangyang , Chen Jingbo , Zong Junqin , Li Jianjian , Liu Jianxiu 
Published: January 2020

Abstract
Background: Areas with saline soils are sparsely populated and have fragile ecosystems, which severely restricts the sustainable development of local economies. Zoysia grasses are recognized as excellent warm-season turfgrasses worldwide, with high salt tolerance and superior growth in saline-alkali soils. However, the mechanism underlying the salt tolerance of Zoysia species remains unknown.
Results: The phenotypic and physiological responses of two contrasting materials, Zoysia japonica Steud. Z004 (salt sensitive) and Z011 (salt tolerant) in response to salt stress were studied. The results show that Z011 was more salt tolerant than was Z004, with the former presenting greater K+/Na+ ratios in both its leaves and roots. To study the molecular mechanisms underlying salt tolerance further, we compared the transcriptomes of the two materials at different time points (0 h, 1 h, 24 h, and 72 h) and from different tissues (leaves and roots) under salt treatment. The 24-h time point and the roots were identified as a significant time point and tissue type. Moreover, GO and KEGG analyses of different comparisons revealed that the key DEGs participating in the salt-stress response belonged to the hormone pathway, various TF families and the DUF family.
Conclusions: Z011 may have improved salt tolerance by reducing Na+ transport from the roots to the leaves, increasing K+ absorption in the roots and reducing K+ secretion from the leaves to maintain a significantly greater K+/Na+ ratio. Twenty-four hours might be a relatively important time point for the salt-stress response of zoysiagrass. The auxin signal transduction family, ABA signal transduction family, WRKY TF family and bHLH TF family may be the most important families in Zoysia salt-stress regulation. This study provides fundamental information concerning the salt-stress response of Zoysia and improves the understanding of molecular mechanisms in salt-tolerant plants.
Path analysis of skin cancer preventive behaviors in rural women based on protection motivation theory
Authors: Kaviani Abdol-Hossain , Khorsandi Mahboobeh , Roozbahani Nasrin 
Published: January 2020

Abstract
Background: Determining factors affecting adopting preventive behaviors of skin cancer is helpful in designing interventions promoting these behaviors. The present study was conducted aimed to analyze preventive behaviors’ path of skin cancer in rural women based on protection motivation theory.
Methods: In this cross-sectional study, 230 rural women in the west of Iran were selected randomly. Data were collected by a valid and reliable questionnaire including demographic information and structures of protection motivation theory by interview with rural women and then analyzed using SPSS 22 and LISREL8.8.
Results: In relation with skin cancer preventive behaviors, 27.8% of women were dressed up when working under the sun, 21.7% used sunscreen cream, 5.7% wore cap and 4.8 wore gloves and sunglasses. Protection motivation theory and per capita income explained 76% of motivation variance and 25% of the variance of skin cancer preventive behaviors. Response efficacy structure was the strongest predictor of the motivation of protection (0/001> p, ß= -0.44) and per capita income (0/001> p, ß= -0.34) and motivation (0/001> p, ß= -0.33) were the strongest predictors of these behaviors.
Conclusions: This study showed that protection motivation theory is efficient in predicting preventive behaviors of skin cancer and promoting interventions of the behaviors can be designed and implemented by this theory. It is also necessary to perform proper planning in promoting these behaviors in people with low per capita income.
Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse
Authors: LI Bei , He Xiaolong , Zhao Yiping , Bai Dongyi , Du Ming , Song Lianjie , Liu Zhuang , Yin Zhenchen , Manglai Dugarjaviin 
Published: January 2020

Abstract
Background: Horse testis development and spermatogenesis are complex physiological processes. Methods: To study these processes, three immature and three mature testes were collected from the Mongolian horse, and six libraries were established using high-throughput RNA sequencing technology (RNA-Seq) to screen for genes related to testis development and spermatogenesis. Results: A total of 16,237 upregulated genes and 8,641 downregulated genes were detected in the testis of the Mongolian horse. These genes play important roles in different developmental stages of spermatogenesis and testicular development. Five genes with alternative splicing events that may influence spermatogenesis and development of the testis were detected. GO (Gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses were performed for functional annotation of the differentially expressed genes. Pathways related to “spermatogenesis,” male gamete generation,” “spermatid development” and “oocyte meiosis” were significantly involved in different stages of testis development and spermatogenesis. Conclusion: Genes, pathways and alternative splicing events were identified with inferred functions in the process of spermatogenesis in the Mongolian horse. The identification of these differentially expressed genetic signatures improves our understanding of horse testis development and spermatogenesis.
Impact of Lesinurad and Allopurinol on Experimental Hyperuricemia in mice: Biochemical, Molecular and Immunohistochemical Study
Authors: Alghamdi Youseef , Soliman Mohamed Mohamed , Nasan Mohamed 
Published: January 2020

AbstractBackground : Hyperuricemia is an abnormal increase in uric acid levels in the blood. It is the cause of gout that manifested by inflammatory arthritis and painful disable. Therefore, current study evaluated the potential ameliorative impact of Lesinurad and Allopurinol on the kidneys of hyperuricemic mice at the biochemical, molecular and cellular levels. Methods : Lesinurad and allopurinol alone or in combination were orally administered to hyperuricemic and control mice for seven consecutive days. Levels of uric acid and blood urea nitrogen, along with antioxidants and inflammatory cytokines (IL-1β and TNF-a) were measured in the serum. The mRNA expression of mouse urate anion transporter-1, glucose transporter 9, organic anion transporters, in renal tissues were examined using quantitative real time PCR (qRT-PCR). Simultaneously, the immunoreactivity of transforming growth factor-beta 1 was examined immunohistochemically. Results : Lesinurad and allopurinol administration resulted in significant decrease in serum levels of uric acid, blood urea nitrogen, xanthine oxidase activity, catalase, glutathione peroxidase and inflammatory cytokines (IL-1β and TNF-a) reported in hyperuricemic mice. Both partially reversed oxonate-induced alterations in renal mURAT-1, mGLUT-9, mOAT-1 and mOAT-3 expressions, as well as alterations in the immunoreactivity of TGF- β1, resulting in the increase of renal uric acid secretion and excretion. The combined administration of lesinurad and ALP restored all altered parameters in a synergistic manner, improving renal function in the hyperuricemic mouse model employed. Conclusion : This study confirmed synergistic ameliorative hypouricemic impact of both lesinurad and allopurinol in the treatment of hyperuricemia in mice at the biochemical, molecular and cellular levels.
Molecular Epidemiology and Hematologic Characterizationof δβ-thalassemia and Hereditary Persistence of Fetal Hemoglobin in 125661 Families of Greater Guangzhou Area, the metropolis of Southern China
Authors: JIANG FAN , Liao Can 
Published: January 2020

Abstract
Background: Individuals with δβ-thalassemia/HPFH and β-thalassemia usually present with intermedia or thalassemia major. No large-scale survey on HPFH/δβ-thalassemia in southern China has been reported to date. The purpose of this study was to examine the molecular epidemiology and hematologic characteristics of these disorders in Guangzhou, the largest city in Southern China, to offer advice for thalassemia screening programs and genetic counseling.
Methods: A total of 125,661 couples participated in pregestational thalassemia screening. 654 subjects with fetal hemoglobin (HbF) level≥5% were selected for further investigation. Gap-PCR combined with Multiplex ligation dependent probe amplification (MLPA) were used to screen for β-globin gene cluster deletions. Gene sequencing for the promoter region of HBG1 /HBG2 gene were performed for all those subjects.
Results: A total of 654 individuals had hemoglobin (HbF) levels≥5%, and 0.12% of the couples were found to be heterozygous for HPFH/δβ-thalassemia, including Chinese Gγ(Aγδβ)0-thal, Southeast Asia HPFH (SEA-HPFH), Taiwanese deletion and Hb Lepore–Boston–Washington. The highest prevalence was observed in the Huadu district and the lowest in the Nansha district. Three cases were identified as carrying β-globin gene cluster deletions, which had not been previously reported. Two at-risk couples (0.0015%) were required to receive prenatal diagnosis. We also found 55 cases of nondeletional-HPFH (nd-HPFH), including 54 with Italian nd-HPFH and one with the A γ-197C-T heterozygous state. It is difficult to discriminate between Chinese G γ( A γδβ) 0 -thal and Italian nd-HPFH carriers using hemoglobin (Hb) analysis.
Conclusions: This study is the first to describe the familial prevalence of HPFH/δβ-thalassemia and the high-risk rate in in Greater Guangzhou Area, and the findings will support the implementation of thalassemia screening for three common deletions by gap-PCR. We also presented a systematic description of genotype-phenotype relationships which will be useful for genetic counseling and prenatal diagnostic services for β-thalassemia intermedia.
Keywords: Prevalence, δβ-thalassemia, HPFH, Guangzhou
Pneumocystis pneumonia occurrence and prophylaxis duration in kidney transplant recipients according to perioperative treatment with rituximab: a single-center, retrospective study
Authors: Kim Young Hoon , Kim Jee Yeon , Kim Dong Hyun , Ko Youngmin , Choi Ji Yoon , Shin Sung , Jung Joo Hee , Park Su-Kil , Kim Sung-Han , Kwon Hyunwook , Han Duck Jong 
Published: January 2020

Abstract
Background Pneumocystis pneumonia (PCP) is a life-threatening fungal infection that can occur in kidney transplantation (KT) recipients. A growing number of KT recipients are receiving perioperative treatment with rituximab, which is associated with prolonged B-cell depletion and possible risk of PCP occurrence; however, the optimal prophylaxis duration according to rituximab treatment is yet unknown. We compared the occurrence of PCP and the duration of prophylaxis in KT recipients according to rituximab treatment. Method We retrospectively analyzed 2110 patients who underwent KT between January 2009 and December 2016. The study cohort was divided into non-rituximab group (n = 1588, 75.3%) and rituximab group (n = 522, 24.7%), the latter of which was defined as recipients who had been treated with rituximab due to pre-operative desensitization or rejection treatment within 6 months after transplant. Results In the rituximab group, the estimated number needed to treat (NNT) for prophylaxis prolongation from 6 to 12 months was 29.0 with a relative risk reduction of 90.0%. In the non-rituximab group, the estimated NNT value was 133.3 and the relative risk reduction was 66.4%. Rituximab treatment (hazard ratio (HR) = 3.09; P < 0.01) and acute rejection (HR = 2.19; P = 0.03) were significant risk factors for PCP in multivariate analysis. Conclusion Our results suggest that maintaining PCP prophylaxis for 12 months may be beneficial in KT recipients treated with rituximab for desensitization or acute rejection treatment.
Milk exosome-derived miRNAs from water buffalo are implicated in immune response and metabolism process
Authors: Chen Zujing , Xie Yueqin , Luo Junyi , Chen Ting , Xi Qianyun , Zhang Yongliang , SUN JIAJIE 
Published: January 2020

Abstract
Background: Buffalo milk represents an indispensable source of nourishment, and recently receives increasing research interest and investment in various countries due to its high nutrient content compared with non-buffalo milks. Exosomes are membranous vesicles of endocytic origin that are found in various breast milks and can mediate intercellular communication with exosomal miRNAs. In comparison with non-buffalo milks, we hypothesized that exosomes from buffalo milk, compared with cow-milk, would yield novel miRNAs that would inform the potential physiological importance of consumption of buffalo-milk exosomes.Results: Here, we isolated exosomes from buffalo and cow milk samples that were obtained at mid-lactation period, and the exosomal miRNA profiles were then generated using RNA-seq. As control, miRNA sequencing data of pig, human and panda milk exosomes were downloaded from GEO database. Finally, a total of 27 milk exosomal miRNA profiles that included 4 buffalo, 4 cow, 8 pig, 4 human and 7 panda were analyzed using the miRDeep2 program. For these datasets there were 558 unique miRNA candidates existed across all species, and the top 10 highly expressed miRNA were evolutionarily conserved across multiple species. Functional analysis revealed that these milk enriched miRNAs targeted 400 putative sites to modulate disease resistance, immune responsiveness and basic metabolism events. In addition, a total of 32 miRNAs in buffalo milk were significantly up-regulated compared with non-buffalo milks, while 16 were significantly down-regulated. Of interest, functional analysis showed that up-regulated miRNA were mainly related to host metabolism processes, while the predicted functions of down-regulated miRNAs were enriched in immune response.Conclusion: In this study, we explored the exosomal miRNAome differences between milks of different animals, expanding the theoretical basis for potential applications of the miRNA-containing vesicles.
Prognosis-related classification and dynamic monitoring of immune status in patients with sepsis: a prospective observational study
Authors: Yin Jun , Chen Yao , Huang Junling , Yan Lei , Kuang Zhongshu , Xue Mingming , Sun Si , Xiang Hao , Hu Yanyan , Dong Zhimin , Tong Chaoyang , Bai Chunxue , song zhenju 
Published: January 2020

Abstract
Background The dynamic monitoring of immune status is crucial to the precise and individualized treatment of sepsis. In this study, we introduced a model to describe and monitor the immune status of sepsis and explored its prognostic value. Methods A prospective observational study was carried out in Zhongshan Hospital, Fudan University, enrolling septic patients admitted between July 2016 and December 2018. Blood samples were collected at day 1 and 3. Serum cytokines levels (TNF-α, IL-2R, IL-6, IL-8 and IL-10) and CD14 + monocyte HLA-DR expression were measured to serve as immune markers. Cut-off values were acquired from ROC curves and serum cytokine level higher than or HLA-DR level lower than cut point was defined as “positive”, otherwise defined as “negative”. Classification of each immune status, namely SIRS, CARS and MARS, was defined by numbers of positive immune markers levels. Changes of immune status from day 1 to 3 were classified into four groups which were Stabilization (SB), Deterioration (DT), Remission (RM) and Non-remission (NR). Results A total of 174 septic patients were enrolled including 50 non-survivors. In univariate analysis, serum levels of TNF-α, IL-2R, IL-6, IL-8 and IL-10 and HLA-DR expression at day 1 and 3 were associated with prognosis. Multivariate analysis discovered IL-10 and HLA-DR expression at day 3 were independent prognostic factors. Patients with MARS at day 1 had the highest mortality rate up to 58.7% among three immune status classifications, so did the patient with MARS at day 3 with mortality rate up to 69.2%. Immune status of 46.1% patients changed from day 1 to 3. Among four groups of immune status changes, DT had the highest mortality rate up to 77.8% followed by NR, RM and SB with mortality rate of 64.7%, 42.9% and 11.2% respectively. Conclusions Patients with severe immune disorder defined as MARS or deterioration of immune status defined as DT have the worst outcomes. The preliminary model of the classification and dynamic monitoring of immune status based on immune markers levels has prognostic values and worthy of further investigation. Trail Registration: ChiCTR-OCC-14005202 (Chinese Clinical Trial Registry), Registered 09 September 2014, http://www.chictr.org.cn/showproj.aspx?proj=4373
Myxoid stroma is associated with postoperative relapse in patients with stage II colon cancer
Authors: Okuyama Takashi , Sameshima Shinichi , Takeshita Emiko , Mitsui Takashi , Noro Takuji , Ono Yuko , Noie Tamaki , Ban Shinichi , Oya Masatoshi 
Published: January 2020

Abstract
Background Fibrosis surrounding cancer cells has been shown to affect cancer cell metastatic behavior. The present study aimed to explore the utility of myxoid stroma as a predictive factor for postoperative relapse in colon cancer patients. Methods The present study retrospectively investigated 169 patients who underwent curative surgical resection of stage II colon cancer. The fibrotic stroma was classified according to Ueno’s criteria, and the patients were divided into the myxoid (MY) group and the non-MY (NMY) group. Results Thirty-two (18.9%) patients had MY. MY was significantly associated with tumor budding (TB) and postoperative relapse (p<0.001 and p<0.001, respectively). Five-year relapse-free and overall survival rates were 52.1% and 94.6%, respectively, in the MY group and 74.6% and 93.3%, respectively, in the NMY group (p<0.001 and p=0.001, respectively). Multivariate analysis showed that both MY and TB were significant risk factors for postoperative relapse (; p<0.001, and p=0.02, respectively). Furthermore, compared with patients with either one of MY or TB, patients with both MY and TB had postoperative relapse significantly more frequently (11.4% vs. 53.8%). Conclusions The present study suggests that MY is a predictive marker for postoperative relapse in patients with stage II colon cancer.
The association between intrinsic capacity and activities of daily living among older adults in China
Authors: Hu Mingyue , Hu Hengyu , Shu Xinhui , Feng Hui 
Published: January 2020

Abstract
Background: The Intrinsic capacity (IC) has been considered the core of older adults’ functional ability. However, there is little study on IC from China, Asia. Methods: Data were drawn from the Long-term Care Demand Assessment (LCDA), a population-based face-to-face survey of 60 years old or over from Southern, Western, Central, Northern, Easton China. Results: A total of 2016 individuals were included in the final analysis. Independent variables were composed of demographic characteristics, four IC domains, health-related factors, and common diseases. The outcome was known as activities of daily living (ADL). Logistic regression analysis was used to explore the association between IC and ADL. Univariate analysis showed that each IC domains were significantly associated with ADL. Multivariate analysis showed that the association between sensory function and ADL was not stable. Nursing home elders (odds ratio [OR] = 0.58; 95% confidence interval [CI] 0.52-0.64) with low nutrition score were more likely to have ADL impairment than community dwellers (OR = 0.86, 95%CI 0.80-0.13). Community dwellers with depression were more likely to suffer ADL impairment (OR = 1.11, 95%CI 1.07-1.14). Conclusion: IC is the core of the ADLs among Chinese older adults. However, the magnitude of the contribution of each IC domain to ADL is partly influenced by living conditions.