bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biosynthesis of vitamin B12 by Propionibacterium freudenreichii subsp. shermanii ATCC 13673 using liquid acid protein residue of soybean as culture medium
Authors: Assis Dener Acosta de , Matte Carla , Aschidamini Bruno , Rodrigues Eliseu , Záchia Ayub Marco Antônio 
Published: May 2020

AbstractVitamin B12 deficiency still persists, mainly caused by low intake of animal food products affecting vegetarians, vegans, and populations of underdeveloped countries. In this study, we investigate the biosynthesis of vitamin B12 by potential probiotic bacterium using an agroindustry residue, the liquid acid protein residue of soybean (LAPRS), as a low‐cost, animal derivate‐free alternative culture medium. Cultures of Propionibacterium freudenreichii subsp. shermanii ATCC 13673 growing in LAPRS for vitamin B12 biosynthesis were studied using the Plackett–Burman experimental approach, followed by a central composite design 22 to optimize the concentration of significant variables. We also performed a proteolytic treatment of LAPRS and evaluated the optimized–hydrolyzed medium influence on the microbial growth and metabolism in shaker flask and bioreactor experiments. In this all‐plant source medium, P. freudenreichii subsp. shermanii produced high concentrations of cells and high amounts of vitamin B12 (0.6 mg/g cells) after process optimization. These results suggest the possibility of producing vitamin B12 by a potential probiotic bacterium in a very cheap, animal derivate‐free medium to address the needs of specific population groups, at the same time reducing the production costs of this essential vitamin.
Design, Synthesis and Bioevaluation of Two Series of 3‐[(1‐Benzyl‐1H‐1,2,3‐triazol‐4‐yl)methyl]quinazolin‐4(3H)‐ones and N‐(1‐Benzylpiperidin‐4‐yl)quinazolin‐4‐amines
Authors: Lan Ta Thu , Anh Duong Tien , Pham‐The Hai , Dung Do Thi Mai , Park Eun Jae , Jang Sun Dong , Kwon Joo Hee , Kang Jong Soon , Thuan Nguyen Thi , Han Sang‐Bae , Nam Nguyen‐Hai 
Published: May 2020

AbstractTwo series of 3‐[(1‐benzyl‐1H‐1,2,3‐triazol‐4‐yl)methyl]quinazolin‐4(3H)‐ones and N‐(1‐benzylpiperidin‐4‐yl)quinazolin‐4‐amines were designed initially as potential acetylcholine esterase inhibitors. Biological evaluation demonstrated that N‐(1‐benzylpiperidin‐4‐yl)quinazolin‐4‐amines significantly inhibited AChE activity. Especially, two compounds of them were found to be the most potent with relative AChE inhibition percentages of 87 % in comparison to donepezil. The docking studies with AChE showed similar interactions between donepezil and four derivatives. N‐(1‐Benzylpiperidin‐4‐yl)quinazolin‐4‐amines also exhibited significant DPPH scavenging effects. The two series of compound also exerted moderate to good cytotoxicity against three human cancer cell lines, including SW620 (human colon cancer), PC‐3 (prostate cancer), and NCI−H23 (lung cancer), with 3‐[(1‐benzyl‐1H‐1,2,3‐triazol‐4‐yl)methyl]quinazolin‐4(3H)‐one being the most cytotoxic agent. 3‐[(1‐Benzyl‐1H‐1,2,3‐triazol‐4‐yl)methyl]quinazolin‐4(3H)‐one significantly induced early apoptosis and arrested the SW620 cells at G2/M phase. From this study, two compounds of N‐(1‐benzylpiperidin‐4‐yl)quinazolin‐4‐amines could serve as new leads for further design and AChE inhibitors, while 3‐[(1‐benzyl‐1H‐1,2,3‐triazol‐4‐yl)methyl]quinazolin‐4(3H)‐one could serve as a new lead for the design and development of more potent anticancer agents.
The roles of SHANK1 in the development of colon cancer
Authors: Wang Lei , Lv Ying , Liu Guoqin 
Published: May 2020

SH3 and multiple ankyrin repeat domains protein 1 (SHANK1) belongs to a family of postsynaptic scaffolding proteins. In this study, we found that SHANK1 was abnormally high expressed in colon cancer tissues compared to normal tissues. Colon cancer patients with low SHANK1 expression had better prognosis. Furthermore, the expression of SHANK1 was knocked down in human colon cancer cell lines HCT116 and HT29 and the role of SHANK1 was investigated in colon cancer tumorigenesis. Our results showed that the knockdown of SHANK1 inhibited the survival and proliferation of both cells. The migration of these two cell lines was significantly reduced and the apoptosis was induced compared with control cells. The Bax/Bcl‐2 ratio in both cell lines that SHANK1 was knocked down was increased, which is a signal that the mitochondrial apoptotic pathway was triggered. In addition, we observed that knockdown of SHANK1 reduced the expression of phosphorylated forms of AKT and mTOR. These data suggested that loss of SHANK1 inhibited viability and induced apoptosis of HCT116 and HT29 cells through the AKT/mTOR signaling pathway. Our data revealed that SHANK1 played important roles in the growth of colon cancer cells and may be used as a novel strategy for colon cancer therapy.Significance of the studyHerein, we reported that SHANK1 was abnormally high expressed in colon cancer tissues and associated with worse prognosis of patients. In addition, knockdown of SHANK1 inhibited viability and induced apoptosis in colon cancer cell lines through AKT/mTOR signaling pathways. These data suggest that SHANK1 may be a new oncogene in colon cancer. This study reveals the role of SHANK1 in addition to neuronal development and cognitive development. And it provides a new potential target for the prediction and treatment of colon cancer.
Bidentate N‐based Ligands for Highly Reusable, Ligand‐coordinated, Supported Pt Hydrosilylation Catalysts
Authors: Chen Linxiao , Ali Iyad S. , Tait Steven L. 
Published: May 2020

AbstractA significant challenge in designing supported metal‐ligand catalysts for solution‐phase reactions is the stabilization of the metal active sites against leaching into solution. Here, we examine alkene hydrosilylation reactions as model systems to improve the stability of highly dispersed Pt using a metal‐ligand coordination strategy on high surface area oxide supports. By evaluating a series of bidentate N‐based ligands, we demonstrate several design strategies to improve stability of the highly dispersed Pt2+ centers against leaching, while maintaining a high level of catalytic activity, selectivity, and recyclability for alkene hydrosilylation batch reactions under mild conditions. These involve a bi‐functional approach to ligand design, which considers interaction to the support and a well‐defined coordination environment for the metal active site. Three strategies are reported: modifying ligands for stronger interaction with oxide surfaces, mixing ligands, and pre‐depositing an “anchoring ligand” to the support before loading the metal‐ligand catalyst. Each of these is successful in enhancing Pt recyclability. Particularly, two Pt‐phenanthroline catalysts exhibit excellent reusability for multiple batch reaction cycles, due to high stability of the active Pt species. Addressing the active site leaching problem significantly enhances the utility of ligand‐coordinated supported metal catalysts as highly stable and selective catalysts for solution‐phase reactions.
Chronic Inhibition of CYP3A is Temporarily Reduced by Each Hemodialysis Session in Patients With End‐Stage Renal Disease
Authors: Egeland Erlend Johannessen , Witczak Bartlomiej J. , Zaré Hasse Khiabani , Christensen Hege , Åsberg Anders , Robertsen Ida 
Published: May 2020

Drug dosing is challenging in patients with end‐stage renal disease. Not only is renal drug elimination reduced, but nonrenal clearance pathways are also altered. Increasing evidence suggest that uremia impacts drug metabolizing enzymes and transporters leading to changes in nonrenal clearance. However, the exact mechanisms are not yet fully understood, and the acute effects of dialysis are inadequately investigated. We prospectively phenotyped cytochrome P450 3A (CYP3A; midazolam) and P‐glycoprotein (P‐gp)/organic anion‐transporting proteins (OATP; fexofenadine) in 12 patients on chronic intermittent hemodialysis; a day after (“clean”) and a day prior to (“dirty”) dialysis. Unbound midazolam clearance decreased with time after dialysis; median (range) reduction of 14% (−3% to 41%) from “clean” to “dirty” day (P = 0.001). Fexofenadine clearance was not affected by time after dialysis (P = 0.68). In conclusion, changes in uremic milieu between dialysis sessions induce a small, direct inhibitory effect on CYP3A activity, but do not alter P‐gp/OATP activity.
Tall willow thickets return to northern Yellowstone
Authors: Painter Luke E. , Tercek Michael T. 
Published: May 2020

AbstractNorthern Yellowstone National Park provides an example of passive restoration, as wetlands and riparian areas there lost most tall willows in the 20th century, due to intensive herbivory by elk (Cervus canadensis). Following large carnivore restoration in the late 1990s, elk numbers decreased, and some researchers reported willows growing taller with reductions in browsing, evidence of a shift toward willow recovery. Others questioned the extent and significance of these changes. To investigate how willow heights have changed in northern Yellowstone since the 1990s, and to assess the importance of browsing as a driver of willow height and canopy cover, we compared data from three time periods: 1988 to 1993 when elk densities were high and most willows very short, 2001 to 2004 when willows may have begun to recover, and 2016 to 2018. We found a strong contrast between sites along streams, compared to wet meadows (meadow sites). Willows in meadow sites did not increase in height, but willows in stream sites increased significantly, exceeding 200 cm mean height in summer by 2001–2004, and in spring by 2016, a height indicative of recovery. Where height did not increase, this was due to loss of annual growth to herbivory. Overall willow height distribution changed from mostly short to become clearly bimodal, with a new peak around 300–400 cm. Bison increased, and in some sites where bison congregate willows remained suppressed at heights below 120 cm, a condition strongly correlated with summer browsing. We also located and measured willow thickets (groups of 5 of more willows >200 cm spring height and <2 m apart) along streams in the study area. We found willow thickets in all stream reaches surveyed, a significant change from past conditions. Thickets occupied >80% of willow patches in some sites, but as little as 22% in others. Tall willow thickets are an important habitat feature and an indicator of willow recovery. Our results demonstrate that the reduction of elk herbivory over the last two decades in northern Yellowstone has allowed willows to grow taller in many places, despite a warming and drying climate, while increased herbivory by bison continues to suppress willows in some locations.
Expanded clinical‐grade membrane‐bound IL‐21/4‐1BBL NK cell products exhibit activity against acute myeloid leukemia in vivo
Authors: Zhao Xiang‐Yu , Jiang Qian , Jiang Hao , Hu Li‐Juan , Zhao Ting , Yu Xing‐Xing , Huang Xiao‐Jun 
Published: May 2020

BackgroundAdoptive NK cell infusion is a promising immunotherapy for acute myeloid leukemia (AML) patients. The aim of this study was to test the activity of clinical‐grade membrane‐bound IL‐21/4‐1BBL‐expanded NK cell products against AML in vivo.MethodsFresh peripheral blood mononuclear cells (PBMCs) were incubated with equal numbers of irradiated membrane‐bound IL‐21/4‐1BBL‐expressing K562 cells for 2–3 weeks to induce clinical‐grade NK cell expansion.ResultsExpansion for 2 and 3 weeks produced ∼4 and 8 × 109 NK cells from 2 × 107 PBMCs. The production of CD107a and TNF‐α in NK cell products in response to AML cell lines and primary blasts was higher than that observed in resting NK cells. The 2‐week expanded NK cell products were xenografted into immunodeficient mice with leukemia and were persistently found in the BM, spleen, liver, lung, and peripheral blood for at least 13 days; furthermore, these expanded products reduced the AML burden in vivo. Compared with matched AML patients with persistent or relapsed minimal residual disease (MRD+) who underwent regular consolidation therapy, MRD+ patients who underwent NK treatment had better overall survival and showed no major adverse events.ConclusionsClinical‐grade mbIL‐21/4‐1BBL‐expanded NK cells exhibited antileukemic activity against AML in vitro and in vivo.
Dietary Supplementation of Tetracarpidium conophorum (African Walnut) Seed Enhances Muscle n‐3 Fatty Acids in Broiler Chickens
Authors: Adeyemi Kazeem D. , Abdulrahman Abdulraheem , Ibrahim Sulyman O. , Zubair Marili F. , Atolani Olubunmi , Badmos Abdulhammed A. 
Published: May 2020

AbstractThe influence of dietary Tetracarpidium conophorum (African Walnut) seed meal (TCSM) on fatty acids, productivity parameters, and physicochemical properties of breast and thigh muscles in broiler chickens are assessed. A total of 180, 28‐d‐old Arbor acre broiler chickens are randomly assigned to dietary treatments containing 0% (control), 2.5%, and 5% w/w TCSM, fed for 28 d, and euthanized. Dietary TCSM reduces (p < 0.05) feed intake, body weight gain (BWG), carcass weight, and abdominal fat. Diet does not affect feed efficiency and hematological parameters. The control birds have higher (p < 0.05) serum total cholesterol and triglycerides than do the supplemented birds. Diet has no effect on pH, water holding capacity, carbonyl and malondialdehyde contents, and organoleptic properties of breast and thigh muscles. The 5% TCSM has higher redness in breast muscle than do other treatments. Dietary TCSM improves (p < 0.05) the concentration of C18:3n‐3 (4.80–8.76% vs 1.56%), C20:5n‐3 (0.54–0.79% vs 0.39%), C22:5n‐3 (0.64–0.89% vs 0.18%), and C22:6n‐3 (0.75–0.97% vs 0.19%), and reduces (p < 0.05) the fat content (2.15–2.45% vs 3.15%) in breast and thigh muscles. Dietary TCSM enhances muscle n‐3 fatty acids without instigating oxidative deterioration, but reduces BWG in broiler chickens.Practical Application: Albeit that broiler meat is rich in polyunsaturated fatty acids (PUFA), its omega 6 (n‐6)/omega 3 (n‐3) is >4. Elevated n‐6/n‐3 could have adverse effect on human physiology thereby promoting the pathogenesis of certain diseases. This heightens the need to enhance the n‐3 PUFA content of broiler meat. Dietary TCSM induced up to a fourfold increase in n‐3 PUFA content of the breast and thigh muscles in broiler chickens. Moreover, dietary TCSM induced up to a tenfold decrease in the n‐6/n‐3 of the breast and thigh muscles in broiler chickens. This finding assumes great significance because the health concerns regarding dietary fat are the foremost factors responsible for the bad image suffered by meat. These results provide insights on the potential of TCSM to improve the nutritional quality without compromising the oxidative shelf life, organoleptic traits, and physicochemical properties of broiler meat.
Electrophoretic measurement of water charge density and ion hydration
Authors: Barger James P. , Dillon Patrick F. 
Published: May 2020

AbstractWater exchange between bulk water and water‐ion complexes will be at equilibrium when the charge density of the complex surface equals the charge density of bulk water, producing a constant radius water‐ion complex. This complex will migrate in an electric field at a velocity proportional to the complex radius. CE velocity is the sum of the complex charge‐dependent velocity and the buffer electro‐osmotic flow. Simultaneous use of both a base (1.07 mM imidazole) and an acid (1.5 mM MOPS) buffer negates EOF at pH 7.4. Electric fields below 300 V/cm (potassium, calcium) and 400 V/cm (magnesium) yield migration velocities with no dehydration of the water‐ion complexes. The number of waters per complex increase with the ion charge density: K+ 1.90, Ca++ 5.90, Mg++ 6.59 waters/ion. The charge densities of the complexes are similar: K+ 1.24, Ca++ 1.43, Mg++ 1.21 e/nm2, for an average bulk water charge density of 1.29 ± 0.11 (SD) e/nm2. The addition of 0.1% Triton increases the number of waters for Mg++ to 25.33 and lowers the charge density to 0.497 e/nm2. High electric field dehydration shows that calcium will be fully dehydrated at 638.3 V/cm and magnesium fully dehydrated at 925.5 V/cm, which occur at 6.15 and 5.78 nm from the membrane. Dehydrated magnesium will then bind to calcium channels leading to decreased smooth muscle activation.
Farmed Mussels in the Northeastern U.S. Exclusive Economic Zone: An Opportunity Too Good to Ignore
Authors: Mizuta Darien D , Wikfors Gary H 
Published: May 2020

Abstract
The United States is the top seafood importer in the world. Nevertheless, opportunities to expand national seafood production, such as offshore aquaculture, are restricted by unclear frameworks for licensing, permitting, and regulating new enterprises. Currently, domestic mussel demand is reliant upon international trade but demand could be met by aquaculture within the Exclusive Economic Zone, especially in suitable areas off the Northeast coast. With national public seafood preferences pointing towards the need for a larger mussel farming industry, science-based efforts to develop offshore farming will contribute to local economies and domestic supply of high-quality, traceable seafood. Beyond a scientific foundation, perceptions that offshore farming represents environmental and privatization threats instead of a national economic development and sustainable opportunity, will have to be addressed. The situation will only change when managers decide to include offshore aquaculture as one of the many legitimate activities for New England's ocean waters, so that spatial planning can be done and conservation measures and offshore farming develop in tandem.
Development of omega‐3 rich eggs through dietary flaxseed and bio‐evaluation in metabolic syndrome
Authors: Shakoor Hira , Khan Muhammad Issa , Sahar Amna , Khan Muhammad Kashif Iqbal , Faiz Furukh , Basheer Ahmad Hafiz 
Published: May 2020

AbstractAn egg is a nutrient‐dense food that contains protein, fats, vitamins, and minerals. It is proven that the consumption of eggs influences serum lipid concentration. Therefore, a study was conducted to investigate the effect of normal and omega‐3 eggs on serum lipids profiles. Lipids were extracted from egg yolks and analyzed for fatty acids content. The present research is a crossover study design in which 20 participants were recruited randomly, and all subjects received three treatments: no eggs, omega‐3 eggs, and normal eggs. However, fasting blood was drawn at baseline and the end of each diet period and analyzed for serum lipids, blood glucose, and insulin level. Omega‐3 egg treatment showed reduction in the serum total cholesterol by 16.57 mg/dl (p < .001), triglyceride by 17.48 mg/dl, and increase in HDL cholesterol concentration by 0.48 mg/dl (p < .001) as compared to no‐egg. A significant (p < .05) reduction in blood pressure by 8.34/8.67 mm/Hg and insulin level was observed due to omega‐3 egg consumption which indicates that omega‐3 fatty acids improve insulin sensitivity. On the other hand, regular egg intake elevates serum total cholesterol and triglycerides concentration but decreases blood pressure. It was concluded that omega‐3‐enriched egg consumption had a positive effect on the serum lipid profile and blood pressure of patients with metabolic syndrome as compared to normal eggs.
Effect of the cleaning process on physical properties for different malting barley seed varieties
Authors: Hetclova Veronika , Jezerska Lucie , Strbova Kristina , Prokes Rostislav , Zegzulka Jiri 
Published: May 2020

AbstractThe aim of the study was to investigate the influence of the barley cleaning process in relation to physical properties. The knowledge of the range of changes in the physical parameters of processed material and their mutual relationships is required for the design and implementation of various technological processes. In this study were compared the input and output commodities in the primary postharvest cleaning process of undesirable components—occurring as admixtures of fine and coarse barley impurities as well as the barley component itself. An efficient cleaning process ensuring barley grain quality is a basic step in beer production. Therefore, seven bred varieties of brewing barley (Malz, Sebastian, Francin, KWS Irina1, KWS Irina2, Bojos, and Laudis) were tested for the qualitative assessment of the cleaning process. Physical parameters such as granulometry, bulk and tapped density, angle of repose, internal and wall friction angle, and flow functions were determined for all samples. In order to identify whether the barley variety or the sample cleaning significantly influences the determined physical properties, two‐way ANOVA was applied. The results imply that barley cleaning had the main influence on wall friction angle, while the barley variety had a significant effect on effective internal friction. Moreover, the mechanical postharvest cleaning process reduces the overall wall friction.
Mitigation of head and neck cancer service disruption during COVID‐19 in Hong Kong through telehealth and multi‐institutional collaboration
Authors: Lee Alex K. F. , Cho Ryan H. W. , Lau Eric H. L. , Cheng Hung K. , Wong Eddy W. Y. , Ku Peter K. M. , Chan Jason Y. K. , Yeung Zenon W. C. 
Published: May 2020

AbstractThe 2019 novel coronavirus disease (COVID‐19) pandemic has been spreading worldwide at an alarming rate. Health‐care workers have been confronted with the challenge of not only treating patients with the virus, but also managing the disruption of health‐care services caused by COVID‐19. In anticipation of outbreak, clinic sessions and operation theater lists have been actively cut back since February 2020 to reduce hospital admissions and clinic attendances. This has severely disrupted health‐care services, leading to accumulating clinic caseload and substantial delays for operations. The head and neck cancer service has been faced with the difficult task of managing the balance between infection risk to health‐care providers and the risk of disease progression from prolonged waiting times. We share our experience in Hong Kong on the mitigation of head and neck cancer service disruption through telehealth and multi‐institution collaboration.
Validation of a self‐administered olfactory and gustatory test for the remotely evaluation of COVID‐19 patients in home quarantine
Authors: Vaira Luigi Angelo , Salzano Giovanni , Petrocelli Marzia , Deiana Giovanna , Salzano Francesco Antonio , De Riu Giacomo 
Published: May 2020

AbstractBackgroundChemosensitive disorders are very frequent in the early stages of COVID‐19 and in paucisymptomatic cases. These patients are typically placed in home quarantine. This study has the aim of validating a new olfactory and gustatory objective evaluation test in these patients.MethodsThirty‐three home‐quarantined COVID‐19 patients have undergone a self‐administered chemosensitive test the day before the control swab. On this occasion, the patients underwent operator‐administered already validated tests. The results were finally compared.ResultsThe differences between the results of the two tests were not significant for both the olfaction (P =.201) and the taste (P =.180).ConclusionThe olfactory and gustatory evaluation by self‐administered test can be considered a valid tool, fundamental for obtaining objective qualitative and quantitative data on the extent of chemosensitive disorders in home‐quarantined COVID‐19 patients.
Respiratory sampling for severe acute respiratory syndrome coronavirus 2: An Overview
Authors: See Anna , Toh Song Tar 
Published: May 2020

AbstractThe novel coronavirus disease 2019 is caused by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and was declared a pandemic in March 2020. A plethora of respiratory sampling methods for SARS‐CoV‐2 viral detection has been used and in the current evolving situation, there is no international consensus on the recommended method of respiratory sampling for diagnosis. Otolaryngologists deal intimately with the upper respiratory tract and a clear understanding of the respiratory sampling methods is of paramount importance. This article aims to provide an overview of the various methods and their evidence till date.