bluuebunny/crossref_metadata_2025_split huggingface dataset:
Daya Dukung Fondasi Tangki 5000 KL Pertamina Region VII Sulawesi, Kota Kendari, Kecamatan Kendari Barat
Authors: Adityawan Muh Handy Dwi , Sarita Umran , Sriyani Rini , Mursidi Baso , Masiki Sultan Machmud Hasan , Ode Wuliman La , Sulha Sulha 
Published: February 2025

Seiring dengan pertambahan penduduk pengguna kendaraan juga meningkat secara signifikan setiap tahunya. Sehingga kebutuhan akan bahan bakar juga meningkat. Selain itu, Meningkatnya aktivitas pertambangan secara regional turut menyumbang peningkatan kebutuhan bahan bakar minyak (BBM). Untuk menjamin ketersedian BBM di Kota Kendari, PT. Pertamina melakukan peningkatan infrastruktur berupa penambahan tangki dengan kapasitas 5000 KL. penelitian ini bertujuan untuk menentukan dimensi fondasi tangki timbun yang sesuai berdasarkan daya dukung tanahnya. Analisi daya dukung fondasi dilakukan dengan terlebih dahulu mengintepretasi data tanah dan data struktur tangki timbun sesuai API 650. Dimensi fondasi awal ditentukan secara trial. Analisis daya dukung berdasarkan data sondir yang dikorelasikan ke nilai kohesi undrained. Hasil analisis menunjukkan bahwa nilai tekanan maksimum yang bekerja pada tanah di bawah fondasi lebih kecil dibandingkan daya dukung ijin dari fondasi. Dengan demikian dimensi fondasi yang telah ditetapkan telah sesuai dan memenuhi persyaratan angka aman
Perceptions of the term “abstinence” among adolescents and young adults: a qualitative study on alcohol non-consumption in Switzerland
Authors: Fernandes Palhares Diana , Chok Lorraine , Suris Joan-Carles , Barrense-Dias Yara 
Published: February 2025

As part of a broader qualitative study aimed at understanding the experiences and opinions of adolescents and young adults (AYA) who do not drink alcohol in Switzerland, participants were questioned about their perceptions of the term “abstinence” in the context of alcohol non-consumption. Twelve focus groups were conducted with 63 participants (36 females, 27 males), aged between 14 years and 20 years. Participants were grouped by gender, age (based on Swiss alcohol laws), and drinking status (non-drinker or drinker). The discussions were recorded and transcribed, and thematic content analysis was used to identify and categorize key themes. The terms “abstinence,” “abstainer,” and “abstention” were generally considered unsuitable when describing young people who do not consume alcohol regardless of the drinking status of the participants. The connotation carried by the terms was mostly perceived as religious, sexual, negative and stigmatizing. “Abstinence” was considered more appropriate for adults who have stopped drinking due to alcohol-related issues. When referring to youths, terms such as “non-drinking,” “non-drinkers” or “alcohol non-consumption” were preferred, especially to better integrate non-drinking youths and positively highlight their choices in preventive and educational initiatives.
Effects of exercise on pulse wave velocity in hypertensive and prehypertensive patients: a systematic review and meta-analysis of randomized controlled trials
Authors: Xi Haoyu , Du Liwen , Li Gen , Zhang Shiyan , Li Xiang , Lv Yuanyuan , Feng Lin , Yu Laikang 
Published: February 2025

BackgroundThis study aimed to examine the effects of exercise on pulse wave velocity (PWV) in hypertensive and prehypertensive patients.MethodsA comprehensive search was performed in PubMed, Cochrane, Embase, Scopus, and Web of Science, covering data up to August 31, 2023. A meta-analysis was conducted to determine the weighted mean difference (WMD) and 95% confidence interval for the effects of exercise on PWV in hypertensive and prehypertensive patients.ResultsA total of 17 studies met the inclusion criteria. Exercise had a significant effect on improving PWV in hypertensive and prehypertensive patients (WMD, −0.93, P = 0.0001). Subgroup analysis showed that aerobic exercise (WMD, −1.29, P = 0.0004) significantly improved PWV in hypertensive and prehypertensive patients. Moreover, aerobic exercise, specifically moderate-intensity aerobic exercise (WMD, −1.69, P = 0.03), conducted for ≥12 weeks (WMD, −1.54, P = 0.002), ≥3 times per week (WMD, −1.44, P = 0.002), ≤60 min per session (WMD, −1.50, P = 0.02), and ≥180 min per week (WMD, −0.57, P = 0.005), was more effective in improving PWV in hypertensive and prehypertensive patients, especially in middle-aged individuals (WMD, −1.78, P &lt; 0.0001).ConclusionTo improve arterial stiffness, hypertensive and prehypertensive patients, particularly middle-aged individuals, are recommended to participate in a minimum of 12 weeks of moderate-intensity aerobic exercise at least 3 times per week for less than 60 min per session, with a goal of 180 min per week being achieved by increasing the frequency of exercise.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=458981, identifier: CRD42023458981.
A quantitative coupling degree model for source-to-sink (S2S) system through entropy weight-coupling theory: a case study of the Chengbei Low Uplift area, offshore Bohai Bay Basin, Eastern China
Authors: Ji Xinyuan , Xu Chunqiang , Li Wenrui , Wang Qiming , Hu Hewei 
Published: February 2025

IntroductionSource-to-sink (S2S) analysis plays a crucial role in sedimentological studies, serving as an effective tool for reconstructing sediment-routing processes and guiding hydrocarbon exploration. Numerous studies on S2S systems have focused on examining the correlations among subsystems by statistically analyzing and characterizing the parameters of the S2S system. However, few studies focus on quantifying the degree of interaction (i.e., the coupling degree) between multiple sub-S2S systems, leading to a biased understanding of coupling mechanisms, S2S processes, and exploration potential.MethodsTo address this issue, we developed a quantitative model for coupling degree based on entropy weight-coupling theory (EW-CT), providing a novel framework for systematically evaluating the interactions within S2S systems. The Paleogene Chengbei Low Uplift (CBLU) in the Bozhong Sag, offshore Bohai Bay Basin (OBBB) in eastern China, serves as a case study to validate the model and examine the multi-hierarchy S2S coupling mechanisms as well as the exploration potential in an ancient uplift system.ResultsThe main findings are: (1) the CBLU is divided into five second-level S2S systems (P/Q/R/S/T), with the study area (S) further subdivided into six third-level sub-S2S systems (S1–S6); (2) there is a positive correlation between EW-CT values and coupling degree in the S2S systems; (3) the coupling degree of the eastern braided-delta S2S systems (S4–S6) exceeds that of the northern fan-delta S2S systems (S1–S3); (4) among the braided-delta S2S systems, the S5 system shows the greatest exploration potential.DiscussionImportantly, this study underscores the broader applicability of the EW-CT model across diverse geological settings, offering a quantitative approach to assess S2S interactions and identify high-potential areas for hydrocarbon exploration. The proposed framework has the potential to enhance sedimentological research and improve exploration strategies in various sedimentary basins worldwide, bridging the gap between theoretical studies and practical applications.
Effect of thyroid stimulating hormone on the prognosis of coronary heart disease
Authors: Ding Ning , Hua Rui , Guo Hanqing , Xu Yu , Yuan Zuyi , Wu Yue , Li Ting 
Published: February 2025

IntroductionClinical studies have shown that thyroid stimulating hormone (TSH) is associated with increased cardiovascular disease risk and mortality. Even within normal ranges, elevated TSH levels have an impact on the cardiovascular system and have been associated with cardiac dysfunction. The aim of our study was to evaluate the predictive value of admission fasting serum TSH levels in patients with coronary heart disease in relation to long-term major adverse cardiovascular events (MACE) and all-cause mortality.MethodA total of 3515 patients with coronary heart disease who met the inclusion criteria were divided into four groups according to the quantile of TSH levels: Group 1 (TSH, 0.34-1.02 mIU/L, n=878); Group 2 (TSH, 1.03-1.71 mIU/L, n=886); Group 3 (TSH, 1.72-2.84 mIU/L, n=880); and Group 4 (TSH, 2.86-5.50 mIU/L, n=873). MACE and all-cause mortality were also compared. TSH concentrations associated with the risk of MACE, all-cause mortality were assessed using continuous scales (restricted quartic splines) and Cox proportional hazards regression models.ResultsA total of 3515 patients with coronary heart disease were eligible for analysis. At a median follow-up of 70 months, patients in group 2 had a lower incidence of MACE compared to the other three groups. All-cause mortality was lower in the 3rd group. Restricted quartic spline analysis also revealed that TSH concentrations were associated with heart failure risk.DiscussionTSH levels have predictive value for adverse cardiovascular events and heart failure in patients with coronary heart disease.
Impact of segmental body composition on metabolic dysfunction-associated fatty liver disease in Chinese children
Authors: Hu Meng-Yuan , Sun Dan-Qin , Yang Fan , Zheng Xiao-Wei , Wu Ning-Xi , Zhang Hao-Yang , Yao Xiao-Die , Zhang Jia-Hui , Zhang Le 
Published: February 2025

PurposeThis study aimed to assess the relationship between regional body composition and metabolic dysfunction-associated fatty liver disease (MAFLD) in Chinese children.MethodsIn this study, 1399 children aged 7–14 years were included. Liver steatosis was assessed using the controlled attenuation parameter (CAP) measured through Fibroscan. MAFLD is defined as the presence of liver steatosis along with either overweight/obesity, prediabetes/diabetes, or at least two metabolic index abnormalities. Regression analyses were applied to assess the relationship between regional body composition and MAFLD in children. Subgroup analyses were performed based on sex and weight.ResultsThe participants had a mean age of 9 years, with 52.11% being boys. Among them, 134 (9.57%) were diagnosed with MAFLD, and 17 (1.22%) had severe fatty liver disease. We found an inverse correlation between the muscle percentage in each region and MAFLD, with the extremities demonstrating the most significant negative correlation (OR: 0.732; 95% CI: 0.634–0.844). Conversely, regional fat was positively associated with MAFLD, with the strongest correlation found in the upper limbs (OR: 3.104; 95% CI: 2.023–4.764). Subgroup analyses showed similar results.ConclusionThe decrease in regional muscle percentage, particularly in the limbs, along with the increase in regional fat percentage, especially in the upper limbs, is associated with a higher probability of developing MAFLD in prepubertal children. Additional prospective studies are needed to strengthen and validate these findings.
Single-cell dual-omics reveals translational and transcriptional landscapes and regulations in oocytes from ovarian endometriosis patients
Authors: Diao Xiaoting , Huang Jiana , Xiang Rui , Zhuang Shaohong , Liang Qiqi , Liang Xiaoyan , Zeng Haitao 
Published: February 2025

IntroductionA significant proportion of women in their reproductive years are afflicted by endometriosis. And one of the major contributing factors to infertility linked to ovarian endometriosis is thought to be oocyte quality. The precise molecular mechanisms are still unknown. Furthermore, because of transcriptional silence, translatome is better able to explain molecular behavior in oocytes than transcriptome sequencing, which has been used widely in recent years.MethodsWe conducted single-cell transcriptome and translatome sequencing on oocytes obtained from patients with ovarian endometriosis, as well as from control subjects with infertility due to tubal or male factors.ResultsFor the first time, we characterized the translational and transcriptional profiles of mRNA in GV-stage oocytes from patients with ovarian endometriosis and control subjects. Our translational analysis identified 2,480 differentially expressed genes in oocytes from ovarian endometriosis patients. Furthermore, we demonstrated that global translational activity in human oocytes is significantly altered by ovarian endometriosis. Key pathways such as "oxidative stress," "oocyte meiosis," and "spliceosome" were identified as critical factors influencing oocyte quality in ovarian endometriosis patients.DiscussionThis study elucidated the molecular characteristics and potential mechanisms underlying poor oocyte quality in patients with ovarian endometriosis. Our findings provided new insights into the pathogenesis of endometriosis-associated infertility and highlighted potential therapeutic targets for improving oocyte quality and reproductive outcomes.
Role of PD-L1 in mediating the effect of lipid on ulcerative colitis: a mediation Mendelian randomization study
Authors: Li Peihong , Wang Yiwen , Hu Hongyi , Sun Boyun 
Published: February 2025

IntroductionRecent evidence suggests that lipids play a crucial role in intestinal metabolic balance and are closely linked to ulcerative colitis (UC). However, the mechanisms underlying their effects remain unclear. This study employed Mendelian randomization (MR) to investigate the relationships among lipids, inflammatory factors, and UC.MethodsWe analyzed data on 179 lipids from the GeneRISK cohort (7,174 individuals), 91 inflammation-related proteins from the EBI GWAS Catalog (14,824 participants), and UC GWAS summary statistics from the FinnGen Biobank (411,317 samples). Associations were assessed using inverse variance weighted (IVW) and Bayesian-weighted MR (BWMR) methods. A mediation analysis was conducted to explore the potential role of inflammatory factors in mediating lipid effects on UC.ResultsMR analysis revealed a significant negative association between sterol ester (27:1/20:4) levels and UC (SNPs = 31; IVW: OR = 0.900 [95% CI: 0.851–0.952], p &lt; 0.001; BWMR: OR = 0.906 [95% CI: 0.849–0.967], p = 0.003). Furthermore, sterol ester (27:1/20:4) was negatively correlated with PD-L1 (SNPs = 30; IVW: OR = 0.961 [95% CI: 0.934–0.990], p = 0.008), and PD-L1 was found to be inversely associated with UC (SNPs = 24; IVW: OR = 0.850 [95% CI: 0.724–0.999], p = 0.048). Mediation analysis suggested that sterol esters (27:1/20:4) may indirectly increase UC risk by downregulating PD-L1 expression. However, the MR analysis results suggest that sterol esters (27:1/20:4) act as a protective factor against UC, which contradicts the mediation analysis. This discrepancy highlights the dual role of PD-L1 in UC pathogenesis.DiscussionPD-L1 may serve as a key mediator in the regulation of UC pathogenesis by sterol esters, but the underlying complex mechanisms require further investigation.
Exploration and verification of circulating diagnostic biomarkers in osteoarthritis based on machine learning
Authors: Wang Xinyu , Liu Tianyi , Sheng Yueyang , Qiu Cheng , Zhang Yanzhuo , Liu Yanqun , Wu Chengai 
Published: February 2025

BackgroundOsteoarthritis (OA) is a prevalent chronic joint condition. This study sought to explore potential diagnostic biomarkers for OA and assess their relevance in clinical samples.MethodsWe searched the GEO database for peripheral blood leukocytes expression profiles of OA patients as a training set to conduct differentially expressed gene (DEG) analysis. Two machine learning algorithms, least absolute shrinkage and selection operator (LASSO) logistic regression and support vector machine-recursive feature elimination (SVM-RFE), were employed to identify candidate biomarkers for OA diagnosis. The performance was assessed using receiver operating characteristic (ROC) curves, and the areas under the curve (AUCs) with 95% confidence interval (CI) were calculated. Furthermore, we gathered clinical peripheral blood samples from healthy donors and OA patients (validation set) to validate our findings. Small interfering RNA and CCK8 proliferation assay were used for experimental verification.ResultsA total of 31 DEGs were discovered, and the machine learning screening found five DEGs that were considered to be candidate biomarkers. Notably, BIRC2 had a very good discriminatory effect among the five candidate biomarkers, with an AUC of 0.814 (95% CI: 0.697-0.915). In our validation set, results showed that the levels of BIRC2 and SEH1L were remarkably higher in healthy donors than OA patients, consistent with the results of the training set. SEH1L owned the largest AUC of 0.964 (95% CI: 0.855-1.000). BIRC2 also displayed a larger AUC of 0.836 (95% CI: 0.618-1.000) in the training set. Knockdown of these two genes could significantly suppress human chondrocyte proliferation.ConclusionTwo novel biomarkers, SEH1L and BIRC2, were indicated to have the capacity to differentiate healthy people from OA patients at the peripheral level. Experiments have shown that knockdown of these two genes could inhibit human chondrocyte proliferation, as verified by cell proliferation assays.
Randomized clinical trial in cancer patients shows immune metabolic effects exerted by formulated bioactive phenolic diterpenes with potential clinical benefits
Authors: Gómez de Cedrón Marta , Moreno-Rubio Juan , de la O Pascual Victor , Alvarez Beatriz , Villarino Marta , Sereno María , Gómez-Raposo César , Roa Silvia , López Gómez Miriam , Merino-Salvador María , Jiménez-Gordo Ana , Falagán Sandra , Aguayo Cristina , Zambrana Francisco , Tabarés Beatriz , Garrido Beatriz , Cruz-Gil Silvia , Fernández Díaz Cristina M. , Fernández Lara P. , Molina Susana , Crespo María Carmen , Ouahid Youness , Montoya Juan José , Ramos Ruíz Ricardo , Reglero Guillermo , Ramírez de Molina Ana , Casado Enrique 
Published: February 2025

BackgroundNutrients, including bioactive natural compounds, have been demonstrated to affect key metabolic processes implicated in tumor growth and progression, both in preclinical and clinical trials. Although the application of precision nutrition as a complementary approach to improve cancer treatments is still incipient in clinical practice, the development of powerful “omics” techniques has opened new possibilities for delivering nutritional advice to cancer patients. Precision nutrition may contribute to improving the plasticity and function of antitumor immune responses.ObjectivesHerein, we present the results of a randomized, prospective, longitudinal, double-blind, and parallel clinical trial (NCT05080920) in cancer patients to explore the immune-metabolic effects of a bioactive formula based on diterpenic phenols from rosemary, formulated with bioactive alkylglycerols (Lipchronic© WO/2017/187000). The trial involved cancer patients, including those with lung cancer (LC), colorectal cancer (CRC), and breast cancer (BC), undergoing chemotherapy, targeted biological therapy, and/or immunotherapy. The main readouts of the study were the analysis of Lip on systemic inflammation, hemogram profile, anthropometry, lipid and glucose profiles, and tolerability. Additionally, a deep immune phenotyping of peripheral blood mononuclear cells (PBMCs) was performed to identify the functional effects of Lip on key mediators of the immune system.ResultsLip was well tolerated. The lung cancer subgroup of patients showed a reduction in biomarkers of systemic inflammation, including the neutrophil-to-lymphocyte ratio (NLR). Furthermore, modulation of key players in the immune system associated with the experimental treatment Lip compared to the control placebo (Pla) treatment was revealed, with particularities among the distinct subgroups of patients. Our results encourage further research to apply molecular nutrition-based strategies as a complementary tool in the clinical management of cancer patients, particularly in the current era of novel immunotherapies.Clinical trial registrationClinicalTrials.gov, identifier NCT05080920
Intradermal delivery of SARS-CoV-2 RBD3-Fc mRNA vaccines via a needle-free injection system induces robust immune responses in rats
Authors: Wang Cenrong , Tang Xin , Jiang Chenghan , Zhang Yu , Han Bo , Sun Yi , Guo Jianfeng , Peng Hanyu , Wang Zihan , Wang Yipeng , Zhang Jialu , Zhang Yong , Jiang Chunlai 
Published: February 2025

IntroductionNeedle-free injection system (NFIS) is easy to operate and can decrease needle phobia. Besides, NFIS can increase the interaction of antigens in a more dispersed manner with immune cell at local injection site, which may improve the immune responses of mRNA vaccines. Although SARS-CoV-2 mRNA vaccines have great success, universal vaccines are urgently needed. Delivering universal mRNA vaccines by NFIS is preferred to combat COVID-19.MethodsRBD3-Fc mRNA expressing BA.4, Delta, and prototype RBD, and human IgG Fc with YTE mutation was designed and synthesized. The safety and immune responses of universal RBD3-Fc naked mRNA and mRNA-LNP vaccines delivered intradermally using NFIS (named GV-01) and intramuscularly via needles were evaluated and compared in rats.ResultsThe prime-boost regimen administered by two routes resulted in potent immune responses and intradermal delivery displays comparable or better performance in terms of binding antibodies, neutralizing antibodies and T cell responses. Naked mRNA vaccines were functional, but less effective than mRNA-LNP vaccines.DiscussionThe above results suggest that RBD3-Fc vaccines are safe and immunogenic and NFIS can be used as an alternative to needles/syringes for the inoculation of mRNA-LNP vaccines to elicit robust systematic immune responses.
A predictive model for early neurological deterioration after intravenous thrombolysis in patients with ischemic stroke
Authors: He Liping , Zhang Meng , Xu Fei , Wu Zhangsong , Chen Huijuan , Li Ying , Chen Ran 
Published: February 2025

ObjectiveIntravenous thrombolysis (IVT) is the treatment of choice for acute ischemic stroke (AIS), but some patients develop early neurological deterioration (END) within 24 h after IVT. Therefore, we aimed to identify predictors of END in AIS patients following treatment with IVT.MethodsWe retrospectively analyzed the clinical data of 621 AIS patients who received IVT with recombinant tissue-type plasminogen activator (rt-PA) at the Stroke Centre of the People’s Hospital of Lu’an City, China, from July 2018 to July 2023. Clinical data, including demographic characteristics, clinical assessment results, underlying diseases, and laboratory indices, were collected at the time of admission. The patients were divided into training and validation cohorts, after which LASSO regression was applied to select the most important predictor variables, and multivariate logistic regression was used to construct a nomogram. The discriminative power of the model was determined by calculating the area under the curve (AUC), and calibration and decision curve analyses (DCA) were performed.ResultsThe platelet-to-lymphocyte ratio (PLR) (OR 1.01, 95% CI 1.01–1.01, p &lt; 0.001), mean platelet corpuscular volume (MPV) (OR 2.12, 95% CI 1.67–2.69, p &lt; 0.001), and admission NIHSS score (OR 1.25, 95% CI 1.16–1.36, p &lt; 0.001) were significantly associated with the development of END. The AUC of the prediction model constructed from these three factors was 0.896 (95% CI 0.862–0.93), and the calibration curve was close to the diagonal.ConclusionThis predictive model can be used for the early identification of the risk of developing END after IVT and development of active interventions to improve the prognosis of AIS.