bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fatty Acids, Tocopherols, Tocotrienols, Phytosterols, Carotenoids, and Squalene in Seed Oils of Hyptis suaveolens, Leonotis nepetifolia, and Ocimum sanctum
Authors: Sahu Pravin Kumar , Chakradhari Suryakant , Sipeniece Elise , Mišina Inga , Qian Ying , Grygier Ania , Rudzińska Magdalena , Patel Khageshwar Singh , Górnaś Paweł 
Published: May 2020

AbstractThe oil yield and composition of fatty acids, tocopherols, tocotrienols, sterols, carotenoids, and squalene in the seeds of three species—Hyptis suaveolens, Leonotis nepetifolia, Ocimum sanctum—belonging to the Lamiaceae family, are studied. The oil yields are 12.1%, 16.1%, and 29.0% in O. sanctum, H. suaveolens, and L. nepetifolia, respectively. The unsaturated fatty acids are a predominant group (86.8–92.1%) in all three investigated plants; however, the profile for each species is unique. The main fatty acid differs as follows: H. suaveolens—linoleic acid (85.8%), L. nepetifolia—oleic acid (58.3%), and O. sanctum—α‐linolenic (48.6%). γ‐Tocopherol accounts for over 97%, 90%, and 93% of the total tocochromanol content (sum of tocopherols and tocotrienols) in H. suaveolens, L. nepetifolia, and O. sanctum, respectively. Two tocotrienol homologues, α and γ, are detected only in L. nepetifolia. β‐Sitosterol is the main detected sterol (38–59%) in all three species. High levels of campesterol (18–20%), Δ5‐stigmasterol (9–21%), and Δ5‐avenasterol (7–12%) are also detected. Squalene is detected only in O. sanctum (45.8 mg/100 g oil). The content of sterols, tocochromanols, and carotenoids in the investigated Lamiaceae plant seed oils ranges between 279.5–576.3, 54.5–66.7, and 0.3–3.1 mg/100 g oil, respectively.Practical Applications: Lamiaceae plants are of medicinal interest due to the presence of a broad spectrum of bioactive molecules. The present study demonstrates that seeds of the species H. suaveolens, L. nepetifolia, and O. sanctum are rich sources of bioactive compounds of lipophilic nature. There is limited knowledge associated with the composition of tocopherols, tocotrienols, sterols, carotenoids, and squalene. The results of the studied medicinal plants may enhance future targeted applications in various sectors.
in vitro Selection of Aptamer for Imidacloprid Recognition as Model Analyte and Construction of a Water Analysis Platform
Authors: Bor Gulsah , Man Ezgi , Ugurlu Ozge , Ceylan Ayse Elcin , Balaban Simge , Durmus Ceren , Pinar Gumus Z. , Evran Serap , Timur Suna 
Published: May 2020

AbstractPesticide use in agriculture is one of the threats to water safety. Therefore, detection of pesticide residues is crucial for human health. Compared to conventional chromatographic methods, aptasensors are promising tools for fast, cheap and sensitive detection of environmental contaminants. To the best of our knowledge, such an aptasensor has not been reported for imidacloprid (Imi) which is one of the most widely used pesticides. In order to meet this demand, we initially selected two novel aptamers designated as ‘Apta‐1’ and ‘Apta‐2’ by graphene oxide‐SELEX (GO‐SELEX) method. Then, these aptamers were used to fabricate the gold electrode‐based aptasensor platforms and characterized by using electrochemical methods such as cyclic voltammetry, and electrochemical impedance spectroscopy as well as X‐Ray photoelectron spectroscopy. It was found that the limit of detection value of Apta‐1 based sensor for the Imi was found better than Apta‐2 based system, although linear ranges were similar. Based on that finding, Apta‐1 based system was further tested against possible interference molecules. The proposed platform was successfully used for detection of very low concentrations of Imi in the range of ng/mL. Thus, it eliminates the need for sample pre‐treatment and enables a practical analysis in real wastewater samples.
Minimal deuterium isotope effects in quantitation of dimethyl‐labeled complex proteomes analyzed with capillary zone electrophoresis/mass spectrometry
Authors: Yan Xiaojing , Sun Liangliang , Dovichi Norman J. , Champion Matthew M. 
Published: May 2020

AbstractStable heavy‐isotope labeling is commonly used in quantitative proteomics. Several common techniques incorporate deuterium (2H) as the heavy isotopic label using reductive amination with formaldehyde. Compared with alternatives, dimethyl labeling reagents are inexpensive and the labeling chemistry is simple and rapid. However, the substitution of hydrogen by deuterium can introduce subtle changes in peptides’ polarities, leading to a shift in chromatographic retention times between deuterated and nondeuterated peptides that can lead to quantification deviations. Capillary zone electrophoresis has emerged as a complementary separation for ESI–MS‐based proteomics, including targeted and quantitative approaches. The extent to which the deuterium isotope effect impacts CZE‐based proteomics, which separates peptides based on their S/N ratios, has not been investigated. To address this issue, CZE was used to analyze dimethyl labeled E. coli tryptic digests in 100 min single‐shot analyses. The median migration time shift was 0.1 s for light versus heavy labeled peptides, which is 2.5% of the peak width. For comparison, nUHPLC–ESI–MS/MS was used to analyze the same sample. In UPLC, deuterated peptides tended to elute earlier than nondeuterated peptides, with a retention shift of 3 s for light versus heavy labeled peptides, which is roughly half the peak width. This shift in separation time did not have a significant effect on quantitation for either method for equal mixing ratios of the light‐intermediate‐heavy isotope labeled samples.
Polyphenolic compounds of amla prevent oxidative stress and fibrosis in the kidney and heart of 2K1C rats
Authors: Rahman Md. Mizanur , Ferdous Khandoker Usran , Roy Shraboni , Nitul Iffat Ara , Mamun Fariha , Hossain Md. Hemayet , Subhan Nusrat , Alam Md Ashraful , Haque Md. Areeful 
Published: May 2020

AbstractAmla (Emblica officinalis Gaertn.) is a natural source of antioxidants and possesses valuable medicinal properties. However, the protective effect of amla in the kidney of two‐kidneys‐one‐clip (2K1C) rats has not been explained sufficiently. This study was performed to evaluate the renoprotective effect of amla fruit powder (2.5% W/W) supplementation in kidneys of 2K1C rats. 2K1C rats increased the remnant kidney wet weight and also increased plasma creatinine and uric acid concentration compared to the control. Amla supplementation ameliorates elevated creatinine and uric acid concentration in plasma of 2K1C rats. Various oxidative stress indicators such as malondialdehyde, nitric oxide (NO), and advanced protein oxidation product (APOP) were also increased in plasma, heart, and kidney tissues in 2K1C rats that were also significantly brought down to normal level by amla supplementation. Moreover, the inflammatory cells entry and fibrosis in the 2K1C rat's tissues were prevented by amla supplementation. These research results suggest that amla may restore plasma antioxidant capacities and prevents oxidative stress, inflammation, and fibrosis in 2K1C rats. Taken these results as a base, clinical supplementation of dried amla powder in diet or juice to the CKD patients would be beneficial.
Metabolic fate of newly developed nondigestible oligosaccharide, maltobionic acid, in rats and humans
Authors: Tanabe Kenichi , Okuda Asuka , Ken Fukami , Yamanaka Natsumi , Nakamura Sadako , Oku Tsuneyuki 
Published: May 2020

AbstractMaltobionic acid (MA), formed by a gluconic acid and glucose linked by an α‐1,4 bond, may have the properties of a nondigestible oligosaccharide. The objective of this study was to elucidate the bioavailability of MA in rats and humans by observing digestion of MA by small intestinal enzymes, the fermentation of MA by gut microbiota, and the effect of adaptation following prolonged ingestion of MA. MA digestion was assessed using brush border membrane vesicles (BBMV) from rat small intestine. A within‐subject repeated measures design was used for ingestion experiments in 10 healthy female participants. After MA ingestion, postprandial plasma glucose and insulin levels, breath hydrogen excretion, and urinary MA were measured. The effect of adaptation following prolonged MA ingestion was investigated in rats. MA was minimally hydrolyzed by BBMV. Ingestion of 10 g of MA by healthy females did not elevate postprandial plasma glucose and insulin levels. Breath hydrogen and urinary MA were negligibly excreted over 8 hr following ingestion. Adaptation to prolonged MA ingestion produced no significant difference in exhaled hydrogen levels over 8 hr following administration compared with controls. MA is a new food material that is highly resistant to digestion and fermentation. It expresses the characteristics of a nondigestible oligosaccharide, including being low energy, improving the flavor of food and juice, and mineral solubilization.
Effects of free and encapsulated transglutaminase on the physicochemical, textural, microbial, sensorial, and microstructural properties of white cheese
Authors: Razeghi Fahimeh , Yazdanpanah Sedigheh 
Published: May 2020

AbstractIn this study, the effect of free and encapsulated transglutaminase (TGase) on physicochemical, textural, microstructural, microbial, and sensorial properties of white cheese was investigated. For this purpose, different types of white cheese incorporated with 20 and 60 ppm free enzyme (F20 and F60) and encapsulated enzyme (E20 and E60) were prepared and then compared with control (C) white cheese without TGase. The results showed that the addition of encapsulated TGase significantly (p ˂ .05) increased protein and fat content, dry matter, nitrogen recovery, and pH, as well as the production yield of cheeses. The hardness of treated samples was increased during the storage time, while the reverse trend was observed for the control sample. F60 and E60 samples showed more oriented and compact structures compared with other samples. Based on the results of sensory evaluation, E60 sample received the highest taste and flavor scores. Generally, the physicochemical, sensorial, and microstructural properties of white chesses were improved by the presence of encapsulated enzyme in the formulation.
Reirradiation with intensity‐modulated radiation therapy for recurrent or secondary head and neck cancer: Meta‐analysis and systematic review
Authors: Lee Jeongshim , Shin In‐Soo , Kim Woo Chul , Yoon Won Sup , Koom Woong Sub , Rim Chai Hong 
Published: May 2020

AbstractBackgroundTo summarize outcomes of reirradiation with intensity‐modulated radiotherapy (IMRT) for recurrent or secondary head and neck cancer (HNC).MethodsPrimary endpoints were 2‐year local control (LC) and overall survival (OS). Studies involving only recurrent nasopharyngeal patients with cancer were excluded.ResultsA total of 17 studies involving 1635 patients were included. Fourteen (82%) of those were retrospective, and 15 (88%) were from single institution. Reirradiation with IMRT produced pooled 2‐year LC and OS rates of 52% (95% confidence interval [CI], 46%‐57%) and 46% (95% CI, 41%‐50%), respectively. In subgroup analyses, the rate of salvage surgery (<42% vs ≥42%) influenced the pooled 2‐year LC rate (45.9% vs 58.5%, P = .011). The pooled rates of late grade ≥ 3 and grade 5 toxicities were 26% (95% CI, 20%‐32%) and 3.1% (95% CI, 2%‐5%), respectively.ConclusionsReirradiation with IMRT was an effective modality compared to historical outcomes in the pre‐IMRT era.
Establishing a threshold for rainfall‐induced landslides by a kinetic energy–duration relationship
Authors: Ferro Vito , Carollo Francesco G. , Serio Maria A. 
Published: May 2020

AbstractMany investigators have attempted to define the threshold of landslide failure, that is, the level of the selected climatic variable above which a rainfall‐induced landslide occurs. Intensity–duration (I–d) relationships are the most common type of empirical thresholds proposed in the literature for predicting landslide occurrence induced by rainfall. Recent studies propose the use of the kinetic power per unit volume of rainfall (J m−2 mm−1) to quantify the threshold of landslides induced by rainfall. In this paper, the relationship between rainfall duration and kinetic power corresponding to landslides triggered by rain was used to propose a new approach to define the threshold for predicting landslide occurrence. In particular, for the first time, a kinetic power per unit volume of rainfall–duration relationship is proposed for defining the minimum threshold needed for landslide failure. This new method can be applied using commonly used relationship for estimating the kinetic power per unit volume of rainfall and a new equation based on the measured raindrop size distribution. The applicability of this last method was tested using the data of rainfall intensity, duration and median volume diameter for 51 landslides in Taiwan. For the 51 landslides, the comparison between the measured pairs' kinetic power–duration and all selected relationships demonstrated that the equation based on the measured raindrop size distribution is the best method to define the landslide occurrence threshold, as it is both a process‐oriented approach and is characterized by the best statistical performance. This last method has also the advantage to allow the forecasting of landslide hazard before the end of the rainfall event, since the rainfall kinetic power threshold value can be exceeded for a time interval less than the event duration.
An enhanced weighted greedy analysis pursuit algorithm with application to EEG signal reconstruction
Authors: Mohagheghian Fahimeh , Deevband Mohammad Reza , Samadzadehaghdam Nasser , Khajehpour Hassan , Makkiabadi Bahador 
Published: May 2020

AbstractIn the past decade, compressed sensing (CS) has provided an efficient framework for signal compression and recovery as the intermediate steps in signal processing. The well‐known greedy analysis algorithm, called Greedy Analysis Pursuit (GAP) has the capability of recovering the signals from a restricted number of measurements. In this article, we propose an extension to the GAP to solve the weighted optimization problem satisfying an inequality constraint based on the Lorentzian cost function to modify the EEG signal reconstruction in the presence of heavy‐tailed impulsive noise. Numerical results illustrate the effectiveness of our proposed algorithm, called enhanced weighted GAP (ewGAP) to reinforce the efficiency of the signal reconstruction and provide an appropriate candidate for compressed sensing of the EEG signals. The suggested algorithm achieves promising reconstruction performance and robustness that outperforms other analysis‐based approaches such as GAP, Analysis Subspace Pursuit (ASP), and Analysis Compressive Sampling Matching Pursuit (ACoSaMP).
Proteomic analysis of underlying apoptosis mechanisms of human retinal pigment epithelial ARPE‐19 cells in response to mechanical stretch
Authors: Yan Fancheng , Gao Meng , Gong Yiyi , Zhang Lin , Ai Nanping , Zhang Jingxue , Chai Yijie , Wu Shen , Liu Qian , Jiang Xian , Deng Haiteng , Liu Wu 
Published: May 2020

AbstractOur previous study demonstrated mechanical stretch (MS) could induce the apoptosis of retinal pigment epithelial (RPE) cells, but the related mechanisms remained unclear. This study was to characterize the protein expression profile in RPE cell line ARPE‐19 exposed to MS, cytochalasin D (CD; an inhibitor of actin polymerization) or CD + MS at 2‐time points (6, 24 hr; n = 3, at each time point) by using proteomics technique. Our data highlighted that compared with control, ECE1 was continuously downregulated in ARPE‐19 cells treated by MS or CD + MS from 6 to 24 hr. Function and protein–protein interaction network analyses showed ATAD2 was downregulated in all three treatment groups compared with control, but successive upregulation of RPS13 and RPL7 and downregulation of AHSG were specifically induced by MS. ATAD2 was enriched in cell cycle; AHSG was associated with membrane organization; RPS13 and RPL7 participated in ribosome biogenesis. Furthermore, transcription factor CREB1 that was upregulated in MS group at 24 hr after treatment, may negatively regulate ATAD2. The expressions of all crucial proteins in ARPE‐19 cells were confirmed by western blot analysis. Overexpression of ATAD2 and AHSG were also shown to reverse the apoptosis of ARPE‐19 cells induced by MS or CD + MS, with significantly decreased apoptotic rates and caspase‐3 activities. Accordingly, our findings suggest downregulation of ATAD2 and AHSG may be potential contributors to the apoptosis of RPE cells induced by MS. Overexpression of them may represent underlying preventive and therapeutic strategies for MS‐induced retinal disorders.
Mass transfer limitations in binderless ZSM‐5 zeolite granules during adsorption of flavour compounds from aqueous streams
Authors: Gernat Deborah C , Rozenbroek Renzo , Brouwer Eric R , van der Wielen Luuk AM , Ottens Marcel 
Published: May 2020

AbstractBACKGROUNDRecently, a new process concept has been proposed to selectively adsorb wort off‐flavours, i.e. aldehydes, from alcohol‐free beers with hydrophobic zeolites.RESULTSIn this work, we investigated the uptake of a mixture of wort flavour compounds (2‐methylpropanal, 2‐methylbutanal, 3‐methylbutanal, furfural, and methional), from a model solution onto binderless hydrophobic ZSM‐5 zeolite granules in order to quantify mass transfer parameters and identify bottlenecks. Subsequently, the homogenous solid diffusion model was employed to regress the effective diffusion coefficients for each molecule and experimental condition, which ranged between 10−15 and 10−13 m2 s−1, indicating strong intraparticle mass transfer limitation. Furthermore, it was found that the effective diffusion coefficient is inversely correlated to the molecules' hydrophobicity, expressed as the logD value and its isotherm affinity constant.CONCLUSIONThese results give valuable insight to design and improve the adsorbent material and an off‐flavour removal unit at industrial scale. © 2020 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Generating simple near‐bipartite bricks
Authors: Kothari Nishad , de Carvalho Marcelo H. 
Published: May 2020

AbstractA brick is a 3‐connected graph such that the graph obtained from it by deleting any two distinct vertices has a perfect matching. A brick is near‐bipartite if it has a pair of edges and such that is bipartite and matching covered; examples are and the triangular prism . The significance of near‐bipartite bricks arises from the theory of ear decompositions of matching covered graphs. The object of this paper is to establish a generation procedure which is specific to the class of simple near‐bipartite bricks. In particular, we prove that every simple near‐bipartite brick has an edge such that the graph obtained from by contracting each edge that is incident with a vertex of degree two is also a simple near‐bipartite brick, unless belongs to any of eight well‐defined infinite families. This is a refinement of the brick generation theorem of Norine and Thomas which is applicable to the class of near‐bipartite bricks. Earlier, the first author proved a similar generation theorem for (not necessarily simple) near‐bipartite bricks; we deduce our main result from this theorem. Our proof is based on a strategy of Carvalho, Lucchesi and Murty and uses several of their techniques and results.
Changes in temperature and precipitation in the instrumental period (1951–2018) and projections up to 2100 in Podgorica (Montenegro)
Authors: Burić Dragan , Doderović Miroslav 
Published: May 2020

AbstractThe article presents the results of the analysis of several temperature and precipitation parameters in the instrumental period in the capital of Montenegro—Podgorica, for the period 1951–2018. In order to use the latest results of several Regional Climate Models (RCMs) developed for the Western Balkans, the results of temperature and precipitation modelling for the period 2011–2100, ALADIN, HIRHAM i RACMO models for RCP4.5 and RCP8.5 scenarios, are also presented. For the observed 68‐year period (1951–2018), the trend calculations clearly show that the temperature is rising. This is indicated by all the analysed temperature parameters (TY, TW, TSp, TSu, TA, TYx, TYn, FD, ID, SU, TD, TR, T35+ and T40+). Although seasonal and annual precipitation totals do not show significant changes in the instrumental period, Podgorica's climate has become more arid and extreme, as the number of days with precipitation ≥1 mm (R1) has significantly decreased. Conversly, the number of days with precipitation has increased ≥40 and 50 mm (R40 and R50). The projected changes in the considered precipitation parameters are in most cases insignificant. The obtained results indicate that there is a high agreement between the temperatures projections of the three models used. Concerning precipitation, it appears that their modelling is more complex, because there are visible qualitative and quantitative differences in the projections of models of future changes in seasonal and annual sums (RY, RW, RSp, RSu and RA), that is, the number of precipitation days (R0.1, R1, R10, R20, R30, R40 and R50). The projections of the used models show that in the future we can expect a warmer climate with more extremes of temperature and precipitation in the grid area to which Podgorica belongs.
The Mediation Effect of Ethical Leadership and Creative Performance: A Social Information Processing Perspective
Authors: Wadei Kwame Ansong , Chen Lu , Frempong Joseph , Appienti William Ansah 
Published: May 2020

AbstractDrawing upon social information processing theory, the study examined how ethical leadership shapes creative performance. Specifically, we tested a theoretical model integrating the sequential roles of psychological safety and creative self‐efficacy. A two‐waved sample of 512 supervisor‐subordinate dyads from frontline employees of three service industries located in Ghana was administered. The results of the hierarchical linear modeling analysis revealed that there is a positive relationship between ethical leadership and creative performance and that psychological safety positively and significantly mediates the relationship between ethical leadership and creative performance. Similarly, creative self‐efficacy significantly and positively mediates the relationship between ethical leadership and creative performance. Moreover, both psychological safety and creative self‐efficacy sequentially mediate the relationship between ethical leadership and creative performance. We discuss the implications of these results for research and practice.