bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tannins from senescent Rhizophora mangle mangrove leaves have a distinctive effect on prokaryotic and eukaryotic communities in a Distichlis spicata salt marsh soil
Authors: Zhang Qiu-Fang , Laanbroek Hendrikus J 
Published: July 2020

ABSTRACTDue to climate warming, tannin-rich Rhizophora mangle migrates into tannin-poor salt marshes, where the tannins interfere with the biogeochemistry in the soil. Changes in biogeochemistry are likely associated with changes in microbial communities. This was studied in microcosms filled with salt marsh soil and amended with leaf powder, crude condensed tannins, purified condensed tannins (PCT), all from senescent R. mangle leaves, or with tannic acid. Size and composition of the microbial communities were determined by denaturing gradient gel electrophoresis, high-throughput sequencing and real-time PCR based on the 16S and 18S rRNA genes. Compared with the control, the 16S rRNA gene abundance was lowered by PCT, while the 18S rRNA gene abundance was enhanced by all treatments. The treatments also affected the composition of the 16S rRNA and 18S rRNA gene assemblies, but the effects on the 18S rRNA gene were greater. The composition of the 18S rRNA gene, but not of the 16S rRNA gene, was significantly correlated with the mineralization of carbon, nitrogen and phosphorus. Distinctive microbial groups emerged during the different treatments. This study revealed that migration of mangroves may affect both the prokaryotic and the eukaryotic communities in salt marsh soils, but that the effects on the eukaryotes will likely be greater.
The biosurfactants iturin, lichenysin and surfactin, from vaginally isolated lactobacilli, prevent biofilm formation by pathogenic Candida
Authors: Nelson Jakline , El-Gendy Ahmed O , Mansy Moselhy S , Ramadan Mohamed A , Aziz Ramy K 
Published: July 2020

ABSTRACT
Lactic acid bacteria (LAB), particularly lactobacilli, are major components of the vaginal microbiota. Lactobacilli are facultative anaerobes forming a critical line of defense against pathogenic microorganisms, including those forming biofilms, such as Candida spp. This study aimed to investigate the anti-adhesion capabilities of vaginal Lactobacillus isolates against biofilms formed by pathogenic Candida species. When the extracellular biosurfactant activities of culture supernatants from 120 Lactobacillus isolates were evaluated by the oil-spreading method, clear spreading zones were recognized. Biofilm formation was quantified by the crystal violet plate assay, and different isolates exhibited anti-adhesion activity that ranged from 65.6to 74.4% inhibition against Candida spp. biofilms. Liquid chromatography high-resolution electrospray ionization mass spectrometry (LC-HRESIMS) identified biosurfactants, extracted from three representative Lactobacillus isolates, as surfactin, iturin and lichenysin. Finally, the distribution of representative genes from six different biosynthetic clusters, related to the production of different biosurfactants, was investigated by the polymerase chain reaction. In conclusion, surfactin, iturin and lichenysin were identified for the first time in vaginal Lactobacillus spp. These biosurfactants, which showed strong anti-adherence activity may be used as promising antibiofilm agents in equipment care to prevent vaginal infections by pathogenic Candida spp. with the prospect of reducing nosocomial infections.
Towards yeast taxogenomics: lessons from novel species descriptions based on complete genome sequences
Authors: Libkind D , Čadež N , Opulente D A , Langdon Q K , Rosa C A , Sampaio J P , Gonçalves P , Hittinger C T , Lachance M A 
Published: July 2020

ABSTRACTIn recent years, ‘multi-omic’ sciences have affected all aspects of fundamental and applied biological research. Yeast taxonomists, though somewhat timidly, have begun to incorporate complete genomic sequences into the description of novel taxa, taking advantage of these powerful data to calculate more reliable genetic distances, construct more robust phylogenies, correlate genotype with phenotype and even reveal cryptic sexual behaviors. However, the use of genomic data in formal yeast species descriptions is far from widespread. The present review examines published examples of genome-based species descriptions of yeasts, highlights relevant bioinformatic approaches, provides recommendations for new users and discusses some of the challenges facing the genome-based systematics of yeasts.
Review of Denonvilliers’ fascia: the controversies and consensuses
Authors: Zhu Xiao-Ming , Yu Guan-Yu , Zheng Nan-Xin , Liu Hui-Min , Gong Hai-Feng , Lou Zheng , Zhang Wei 
Published: July 2020

AbstractThe Denonvilliers’ fascia (DVF) plays an important role in rectal surgery because of its anatomic position and its relationship to the surrounding organs. It affects the surgical plane anterior to the rectum in the procedure of total mesorectal excision (TME). Anatomical and embryological studies have helped us to understand this structure to some extent, but many controversies remain. In terms of its embryonical origin, there are three mainstream hypotheses: peritoneal fusion of the embryonic cul-de-sac, condensation of embryonic mesenchyme, and mechanical pressure. Regarding its architecture, the DVF may be a single, two, or multiple layers, or a composite single-layer structure. In women, most authors deem that this structure does exist but they are willing to call it the rectovaginal septum rather than the DVF. Operating behind the DVF is supported by most surgeons. This article will review those mainstream studies and opinions on the DVF and combine them with what we have observed during surgery to discuss those controversies and consensuses mentioned above. We hope this review may help young colorectal surgeons to have a better understanding of the DVF and provide a platform from which to guide future scientific research.
Association of Physical Activity, Sedentary Behavior, and Sleep With Unhealthy Aging: Consistent Results for Device-Measured and Self-reported Behaviors Using Isotemporal Substitution Models
Authors: Ortolá Rosario , García-Esquinas Esther , Cabanas-Sánchez Verónica , Migueles Jairo H , Martínez-Gómez David , Rodríguez-Artalejo Fernando 
Published: July 2020

Abstract

Background
We examined the association of time allocation among physical activity (PA), sedentary behavior (SB), and sleep with unhealthy aging (UA), using both accelerometry and self-reports.

Method
We used cross-sectional data from 2312 individuals aged 65 years and older. Physical activity, SB, and sleep were ascertained by both wrist accelerometers and validated questionnaires, and UA was measured with a 52-item health-deficit accumulation index. Analyses used isotemporal substitution linear regression models.

Results
Less deficit accumulation was observed when the distribution of activities was 30 min/d less of SB and 30 min/d more of PA for both accelerometer (fully adjusted β [95% CI]: –0.75 [–0.90, –0.61]) and self-reports (–0.55 [–0.65, –0.45]), as well as less long sleep and more PA (accelerometer: –1.44 [–1.86, –1.01]; self-reports: –2.35 [–3.35, –1.36]) or more SB (accelerometer: –0.45 [–0.86, –0.05]; self-reports: –1.28 [–2.29, –0.28]), less normal sleep and more moderate-to-vigorous PA (accelerometer: –1.70 [–2.28, –1.13]; self-reports: –0.65 [–0.99, –0.31]), and less accelerometer light PA and more moderate-to-vigorous PA (–1.62 [–2.17, –1.07]). However, more deficit accumulation was observed when less sleep was accompanied by either more SB or more light PA in short sleepers. Self-reports captured differential associations by activity: walking appeared to be as beneficial as more vigorous activities, such as cycling or sports, and reading was associated with less UA than more mentally passive SBs, such as watching TV.

Conclusions
More PA was associated with less UA when accompanied by less SB time or sleep in long/normal sleepers, but not in short sleepers, where the opposite was found. Accelerometry and self-reports provided consistent associations.
Development and Psychometric Testing of the Mealtime Engagement Scale in Direct Care Providers of Nursing Home Residents With Dementia
Authors: Liu Wen , Batchelor Melissa , Williams Kristine 
Published: July 2020

Abstract

Background and Objectives
Mealtime engagement is defined as verbal and nonverbal assistance provided by caregivers to guide and motivate care recipients in eating. Quality mealtime engagement is critical to improve mealtime difficulties and intake among older adults with dementia requiring eating assistance. Few tools are feasible and valid to measure mealtime engagement. This study developed and tested the Mealtime Engagement Scale (MES).

Research Design and Methods
Items were developed based on literature review and expert review and finalized based on content validity and corrected item-total correlation. A secondary analysis of 87 videotaped observations capturing 18 nursing home staff providing mealtime care to residents with dementia was conducted. Internal consistency, interrater reliability, and intrarater reliability were assessed. Concurrent and convergent validity were examined through correlation (rs) with the Relational Behavior Scale (RBS) and the Mealtime Relational Care Checklist (M-RCC), respectively.

Results
The 18-item MES was developed with adequate content validity (Scale-content validity index [CVI] = 1.00; Scale-CVI/Average = 0.962–0.987). Each item is scored from 0 (never) to 3 (always). The total scale score ranges from 0 to 54. Higher scores indicate greater mealtime engagement. The MES had very good internal consistency (Cronbach’s α = 0.837), outstanding interrater reliability (interclass correlation = 0.920), outstanding intrarater reliability (interclass correlation = 0.956), adequate concurrent validity based on strong correlation with the RBS (rs = 0.821, p &lt; .001), and fair convergent validity based on weak correlation with the M-RCC (rs = 0.219, p = .042).

Discussion and Implications
Findings provide preliminary psychometric evidence of MES to measure mealtime engagement. Future testing is needed among more and diverse samples in different care settings to accumulate psychometric evidence.
Managing ancillary care in resource-constrained settings: Dilemmas faced by frontline HIV prevention researchers in a rural area in South Africa
Authors: Nkosi Busisiwe , Seeley Janet , Chimbindi Natsayi , Zuma Thembelihle , Kelley Maureen , Shahmanesh Maryam 
Published: July 2020

Abstract

Background
We describe the findings from a research ethics case study, linked with a team evaluating a package of intervention services to prevent HIV infection in adolescent girls and young women (AGYW) living in a rural and poor setting of KwaZulu-Natal, South Africa.

Methods
We conducted qualitative interviews (n=77) with members of the linked research team evaluating the intervention programme, programme implementing staff, AGYW enrolled in the intervention programme, caregivers, ethics committee members, Public Engagement officers, community advisory board members and community stakeholders. Data were analysed iteratively using thematic framework analysis. Themes were determined by the study aims combined with an inductive development of codes emerging from the data.

Results
The findings show that the burden of providing ancillary care fell primarily on the shoulders of frontline researchers and programme staff. Dilemmas around responding to gender-based violence illustrated the limits of ‘referral to services’ as a solution for meeting ancillary care obligations in contexts with barriers to basic health and social services.

Conclusion
Our findings show important gaps in meeting ancillary care needs. Participants’ needs required social and economic support which frontline researchers and implementing partners were not able to meet, causing moral distress.
Trp: a conserved aromatic residue crucial to the interaction of a scorpion peptide with sodium channels
Authors: Xu Yijia , Sun Jianfang , Yu Yue , Kong Xiaohua , Meng Xiangxue , Liu Yanfeng , Cui Yong , Su Yang , Zhao Mingyi , Zhang Jinghai 
Published: July 2020

Abstract
Anti-tumour-analgesic peptide (AGAP), one scorpion toxin purified from Buthus martensii Karsch, was known as its analgesic and anti-tumour activities. Trp38, a conserved aromatic residue of AGAP, might play important roles in its interaction with sodium channels. In this study, a mutant W38F was generated and effects of W38F were examined on hNav1.4, hNav1.5 and hNav1.7 by using whole-cell patch-clamp, which were closely associated to the biotoxicity of skeletal and cardiac muscles and pain signalling. The data showed that W38F decreased the inhibition effects of peak currents of hNav1.7, hNav1.4 and hNav1.5 compared with AGAP, notably, W38F reduced the analgesic activity compared with AGAP. The results suggested that Trp38 be a crucial amino acid involved in the interaction with these three sodium channels. The decreased analgesic activity of W38F might result from its much less inhibition of hNav1.7. These findings provided more information about the relationship between structure and function of AGAP and may facilitate the modification of other scorpion toxins with pharmacological effects.
Hydrogels: From Controlled Release to a New Bait Delivery for Insect Pest Management
Authors: Tay Jia-Wei , Choe Dong-Hwan , Mulchandani Ashok , Rust Michael K 
Published: July 2020

AbstractHere, we review the literature on the development and application of hydrogel compounds for insect pest management. Researchers have used hydrogel compounds for the past few decades to achieve the controlled release of various contact insecticides, but in recent years, hydrogel compounds have also been used to absorb and deliver targeted concentrations of toxicants within a liquid bait to manage insect pests. The highly absorbent hydrogel acts as a controlled-release formulation that keeps the liquid bait available and palatable to the target pests. This review discusses the use of various types of hydrogel compounds in pest management based on different environmental settings (e.g., agricultural, urban, and natural areas), pest systems (e.g., different taxa), and modes of insecticide delivery (e.g., spray vs bait). Due to their unique physicochemical properties, hydrogel compounds have great potential to be developed into new and efficacious pest management strategies with minimal environmental impact. We will also discuss the future research and development of hydrogels in this review.
Relationship Between the Distribution and Biodiversity of Sand Flies (Diptera: Psychodidae) With the Incidence of Zoonotic Cutaneous Leishmaniasis in Endemic Foci of Golestan Province, Iran
Authors: Sofizadeh Aioub , Akbarzadeh Kamran , Allah Kalteh Ehsan , Karimi Fatemeh 
Published: July 2020

AbstractZoonotic cutaneous leishmaniasis (ZCL) is prevalent in Golestan Province, Iran. The current study determined the relationship between the distribution and biodiversity of sand flies with cutaneous leishmaniasis at 14 villages in plain and hillsides areas. In each village from July to September 2017, 60 sticky traps and 2 CDC light traps were laid. Spearman and Mann–Whitney tests were used to determine the relationship between the incidence of ZCL and the abundance of different species of sand flies. Simpson, Shannon-Wiener, Evenness, and Margalef indices were calculated to estimate the diversity of species. A total of 5,295 phlebotomine sand flies were collected, comprising 10 species of the genus Phlebotomus (3,947 flies) and 7 species of genus Sergentomyia (1,248 flies). The abundance of sand flies and incidence of ZCL in plain areas were greater than that of hillsides areas (P = 0.013, P = 0.002). There was a significant correlation between the incidence of ZCL and the abundance of Phlebotomus papatasi (r = 0.72, P = 0.004) and P. caucasicus groups (P = 0.006; 0.022). In the Shannon-Wiener index, the rest of the biodiversity indices were reduced in higher-altitude areas. Increasing Shannon-Wiener index showed higher diversity of sand flies in higher-altitude areas. Data of the reported cases of leishmaniasis in plain areas can reveal the relationship between less diversity index (Shannon-Wiener), higher dominant diversity index (Simpson), and incidence of leishmaniasis in these areas.
Rosiglitazone ameliorates radiation-induced intestinal inflammation in rats by inhibiting NLRP3 inflammasome and TNF-α production
Authors: Hu Liqiong , Chen Hao , Zhang Xingliang , Feng Zhencheng , Zhang Haifeng , Meng Qingqi 
Published: July 2020

Abstract
Radiation-induced acute intestinal injury is a common and serious occurrence following abdominal and pelvic irradiation. The Nod-like receptor protein 3 (NLRP3)-dependant inflammasome and inflammation activation is crucial in this process. In a pre-experimental design of radiation-induced intestinal injury, we found that rosiglitazone inhibited caspase-1 which is a key marker of inflammasome activation. The purpose of the present study was to clarify the inhibitory effect of rosiglitazone on the NLRP3 inflammasome both in vivo and in vitro. Radiation-induced intestinal injury after rosiglitazone treatment, and the expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), caspase-1 and NLRP3 in a radiation-induced intestinal injury model in a rat and macrophages were observed. We found that rosiglitazone ameliorated radiation-induced intestinal injury in rats by suppressing the expression of caspase-1, NLRP3, IL-1β and TNF-α. Treatment with rosiglitazone in vitro reduced the expression of NLRP3, and the NLRP3 activator monosodium urate (MSU) reversed the inhibition of IL-1β and TNF-α by rosiglitazone in macrophages. MSU reversed the protective effect of rosiglitazone on radiation-induced intestinal injury in rats by reversing the rosiglitazone-induced inhibition of IL-1β and TNF-α. Taken together, these findings indicate that the peroxisome proliferator-activated receptor gamma (PPARγ) agonist, rosiglitazone, ameliorates radiation-induced intestine inflammation in rats via inhibiting the induction of the NLRP3-dependent inflammasome in macrophages.
Effects of MOPP Gear on SAM Medical Junctional Tourniquet Application: A Prospective, Randomized Control Trial
Authors: Wagner Eric M , Naylor Jason F , Ahern Brian J , Gendron Brett C , April Michael D , Schauer Steven G 
Published: July 2020

AbstractIntroductionHemorrhage is the leading cause of preventable death on the battlefield, and hemostasis is particularly challenging to achieve at junctional sites such as the axillary or inguinal regions. Mission-oriented protective posture (MOPP) gear, as worn most recently in Syria to guard against chemical weapons, can make the performance of technical skills more challenging still. The objective of this study was to evaluate how wearing MOPP gear affects the application time of the SAM Medical Junctional Tourniquet (SJT) by U.S. Army combat medics.Materials and MethodsWe conducted a prospective, randomized control trial evaluating time for SJT application between participants wearing MOPP versus those not wearing MOPP. Secondary outcomes included SJT application success rate and participant appraisal of SJT application difficulty assessed with five-point Likert items, between groups. Participants placed SJTs on robotic simulation mannequins with a penetrating inguinal injury.ResultsIn April 2019, we enrolled 49 combat medics. Most participants were male (77.5%), had a median age of 25 (interquartile range 23–28), and in the grade of E4 or less (63.3%). Mean SJT application times in seconds were higher among those wearing MOPP versus those who were not (223.1 versus 167.2; 95% confidence interval for difference in means 5.293, 106.374; P = 0.03). Participants wearing MOPP had a less successful application rate overall, but this difference was not statistically significant (64.3% versus 81.0%, P = 0.34). Compared to participants not wearing MOPP, those wearing MOPP agreed that SJT application was difficult (4 versus 3, P = 0.03), what they were wearing affected SJT application (4 versus 2, P = 0.01), and it was difficult to use their hands during SJT application (4 versus 1, P &lt; 0.001).ConclusionsWearing military MOPP gear significantly prolongs the amount of time required for combat medics to apply an SJT on a simulated casualty with a penetrating inguinal injury. This study highlights the importance of incorporating MOPP gear into medical training scenarios to improve skills competency while wearing these protective garments.
On the Trail of a Knight of Santiago: Collecting Music and Mapping Knowledge in Renaissance Europe
Authors: Honisch Erika Supria , Escrivà-Llorca Ferran , Knighton Tess 
Published: July 2020

Abstract
The 2011 discovery that the substantial collection of sixteenth-century printed partbooks preserved in Madrid’s Real Conservatorio Superior de Música originally belonged to an Austrian diplomat, Wolfgang Rumpf, has opened up new perspectives on the formation of early modern music libraries. Employed by the Holy Roman Emperor Rudolf II, Rumpf was also an informant of Philip II of Spain, who rewarded him with induction into the chivalric Order of Santiago. The Order, headquartered at Uclés, received the bulk of Rumpf’s music library after his death.
This article uses Rumpf’s library, and the catalogue he commissioned from the Imperial Librarian Hugo Blotius, to shed light on the music book’s place in early modern material culture, and music’s place in an expanding world of knowledge. Rumpf’s partbooks were not, in the first instance, intended for performance; they reflect instead his assiduous efforts to assemble a ‘universal library’ in which music books formed an integral part.
PINCER: improved CRISPR/Cas9 screening by efficient cleavage at conserved residues
Authors: Veeneman Brendan , Gao Ying , Grant Joy , Fruhling David , Ahn James , Bosbach Benedikt , Bienkowska Jadwiga , Follettie Maximillian , Arndt Kim , Myers Jeremy , Zhong Wenyan 
Published: July 2020

Abstract
CRISPR/Cas9 functional genomic screens have emerged as essential tools in drug target discovery. However, the sensitivity of available genome-wide CRISPR libraries is impaired by guides which inefficiently abrogate gene function. While Cas9 cleavage efficiency optimization and essential domain targeting have been developed as independent guide design rationales, no library has yet combined these into a single cohesive strategy to knock out gene function. Here, in a massive reanalysis of CRISPR tiling data using the most comprehensive feature database assembled, we determine which features of guides and their targets best predict activity and how to best combine them into a single guide design algorithm. We present the ProteIN ConsERvation (PINCER) genome-wide CRISPR library, which for the first time combines enzymatic efficiency optimization with conserved length protein region targeting, and also incorporates domains, coding sequence position, U6 termination (TTT), restriction sites, polymorphisms and specificity. Finally, we demonstrate superior performance of the PINCER library compared to alternative genome-wide CRISPR libraries in head-to-head validation. PINCER is available for individual gene knockout and genome-wide screening for both the human and mouse genomes.