bluuebunny/crossref_metadata_2025_split huggingface dataset:
Household economic burden and outcomes of patients with schizophrenia after being unlocked and treated in rural China
Authors: Xu L. , Xu T. , Tan W. , Yan B. , Wang D. , Li H. , Lin Y. , Li K. , Wen H. , Qin X. , Sun X. , Guan L. , Bass J.K. , Ma H. , Yu X. 
Published: December 2019

Abstract

Aims
Patients with severe mental disorders in low-resource settings have limited access to services, resulting in overwhelming caregiving burden for families. In extreme cases, this has led to the long-term restraining of patients in their homes. China underwent a nationwide initiative to unlock patients and provide continued treatment. This study aims to quantify household economic burden in families after unlocking and treatment, and to identify factors associated with increased burden due to schizophrenia.

Methods
A total of 264 subjects were enrolled from three geographically diverse provinces in 2012. Subjects were patients with schizophrenia who were previously put under restraints and had participated in the ‘unlocking and treatment’ intervention. The primary outcome was the current household economic burden, obtained from past year financial information collected through on-site interview. Patient disease characteristics, treatment, outcomes and family caregiving burden were collected as well. Univariate and multivariate linear regression were used to construct risk factor models for indirect economic burden.

Results
After participating in the intervention, 85% of patients continued to receive mental health services, 70% used medication as prescribed and 80% were never relocked. Family members reported significantly decreased caregiving burden after receiving the intervention. Mean direct and indirect household economic burdens were CNY963 (US$31.7) and CNY11 724 (US$1670) per year, respectively, while family total income was on average CNY12 108 (US$1913) per year. Greater disease severity and poorer patient psychosocial function at time of study were found to be independent factors related to increased indirect burden.

Conclusions
The ‘unlocking and treatment’ intervention has improved the lives of patients and families. Indirect burden due to disease is still a major economic issue that needs to be addressed, potentially through improving treatment and patient functioning. Our findings contribute to the unravelling and eventual elimination of chronic restraining of mentally ill patients in low-resource settings.
First Steps of the Mexico Twin Registry (RGMex-MexTR) at the University of Guadalajara
Authors: Cervantes-Cardona Guillermo A. , Cervantes-Nápoles Tania E. , Cervantes-Guevara Gabino , Manzo-Palomera Olga R. , González-Ojeda Alejandro , Silva-González Gabriela , Fuentes-Orozco Clotilde , Sánchez-Michel Ángel , Nápoles-Echauri Adriana 
Published: December 2019

AbstractDespite the well-known relevance of twin studies in the medical and social sciences and the growing number of twin registries throughout the world, Latin America has not fully incorporated into the twin research community. We describe the first steps taken toward developing a twin registry in Mexico: its aim, organization, recruiting potential and main short-term objectives.
Folate and vitamin B-12 deficiencies additively impaire memory function and disturb the gut microbiota in amyloid-β infused rats
Authors: Park Sunmin , Kang Sunna , Sol Kim Da 
Published: December 2019

Abstract. Folate and vitamin B12(V-B12) deficiencies are associated with metabolic diseases that may impair memory function. We hypothesized that folate and V-B12 may differently alter mild cognitive impairment, glucose metabolism, and inflammation by modulating the gut microbiome in rats with Alzheimer’s disease (AD)-like dementia. The hypothesis was examined in hippocampal amyloid-β infused rats, and its mechanism was explored. Rats that received an amyloid-β(25–35) infusion into the CA1 region of the hippocampus were fed either control(2.5 mg folate plus 25 μg V-B12/kg diet; AD-CON, n = 10), no folate(0 folate plus 25 μg V-B12/kg diet; AD-FA, n = 10), no V-B12(2.5 mg folate plus 0 μg V-B12/kg diet; AD-V-B12, n = 10), or no folate plus no V-B12(0 mg folate plus 0 μg V-B12/kg diet; AD-FAB12, n = 10) in high-fat diets for 8 weeks. AD-FA and AD-VB12 exacerbated bone mineral loss in the lumbar spine and femur whereas AD-FA lowered lean body mass in the hip compared to AD-CON(P < 0.05). Only AD-FAB12 exacerbated memory impairment by 1.3 and 1.4 folds, respectively, as measured by passive avoidance and water maze tests, compared to AD-CON(P < 0.01). Hippocampal insulin signaling and neuroinflammation were attenuated in AD-CON compared to Non-AD-CON. AD-FAB12 impaired the signaling (pAkt→pGSK-3β) and serum TNF-α and IL-1β levels the most among all groups. AD-CON decreased glucose tolerance by increasing insulin resistance compared to Non-AD-CON. AD-VB12 and AD-FAB12 increased insulin resistance by 1.2 and 1.3 folds, respectively, compared to the AD-CON. AD-CON and Non-AD-CON had a separate communities of gut microbiota. The relative counts of Bacteroidia were lower and those of Clostridia were higher in AD-CON than Non-AD-CON. AD-FA, but not V-B12, separated the gut microbiome community compared to AD-CON and AD-VB12(P = 0.009). In conclusion, folate and B-12 deficiencies impaired memory function by impairing hippocampal insulin signaling and gut microbiota in AD rats.
Intensified Ocean Deoxygenation During the end Devonian Mass Extinction
Authors: Liu Jiangsi , Luo Genming , Lu Zunli , Lu Wanyi , Qie Wenkun , Zhang Feifei , Wang Xiangdong , Xie Shucheng 
Published: December 2019

AbstractThe end‐Devonian mass extinction (~359 Ma) substantially impacted marine ecosystems and shaped the roots of modern vertebrate biodiversity. Although multiple hypotheses have been proposed, no consensus has been reached about the mechanism inducing this extinction event. In this study, I/Ca ratio of carbonate was used to unravel the changes in local oxygen content of the upper water column during this critical interval. The Devonian‐Carboniferous boundary was recorded in two shallow water carbonate sections in south China. I/Ca values at both locations show a clear decline in the Middle and UpperSiphonodella praesulcataconodont zones, which coincides with a positive shift in carbonate carbon isotope composition and a negative shift in nitrogen isotope composition. These results suggest that deoxygenation was intensified during this critical interval, which likely influenced shallow water habitats, lending support to the notion that oxygen deficiency likely was a direct mechanism impacting the end‐Devonian mass extinction.
Physiological levels of the PTEN-PI3K-AKT axis activity are required for maintenance of Burkitt lymphoma
Authors: Gehringer Franziska , Weissinger Stephanie Ellen , Möller Peter , Wirth Thomas , Ushmorov Alexey 
Published: December 2019

Abstract
In addition to oncogenic MYC translocations, Burkitt lymphoma (BL) depends on the germinal centre (GC) dark zone (DZ) B cell survival and proliferation programme, which is characterized by relatively low PI3K-AKT activity. Paradoxically, PI3K-AKT activation facilitates MYC-driven lymphomagenesis in mice, and it has been proposed that PI3K-AKT activation is essential for BL. Here we show that the PI3K-AKT activity in primary BLs and BL cell lines does not exceed that of human non-neoplastic tonsillar GC DZ B cells. BLs were not sensitive to AKT1 knockdown, which induced massive cell death in pAKThigh DLBCL cell lines. Likewise, BL cell lines show low sensitivity to pan-AKT inhibitors. Moreover, hyper-activation of the PI3K-AKT pathway by overexpression of a constitutively active version of AKT (myrAKT) or knockdown of PTEN repressed the growth of BL cell lines. This was associated with increased AKT phosphorylation, NF-κB activation, and downregulation of DZ genes including the proto-oncogene MYB and the DZ marker CXCR4. In contrast to GCB-DLBCL, PTEN overexpression was tolerated by BL cell lines. We conclude that the molecular mechanisms instrumental to guarantee the survival of normal DZ B cells, including the tight regulation of the PTEN-PI3K-AKT axis, also operate in the survival/proliferation of BL.
Rationally engineered 3D-dendritic cell-like morphologies of LDH nanostructures using graphene-based core–shell structures
Authors: Sarigamala Karthik Kiran , Shukla Shobha , Struck Alexander , Saxena Sumit 
Published: December 2019

AbstractFunctionalization of graphene-based materials using chemical moieties not only modify the electronic structure of the underlying graphene but also enable in limited enhancement of targeted properties. Surface modification of graphene-based materials using other nanostructures enhances the effective properties by minimally modifying the properties of pristine graphene backbone. In this pursuit, we have synthesized bio-inspired hierarchical nanostructures based on Ni–Co layered double hydroxide on reduced graphene oxide core–shells using template based wet chemical approach. The material synthesized have been characterized structurally and electrochemically. The fabricated dendritic morphology of the composite delivers a high specific capacity of 1056 Cg−1. A cost effective solid state hybrid supercapacitor device was also fabricated using the synthesized electrode material which shows excellent performance with high energy density and fast charging capability.
Molecular anatomy and pathogenic actions of Helicobacter pylori CagA that underpin gastric carcinogenesis
Authors: Takahashi-Kanemitsu Atsushi , Knight Christopher T. , Hatakeyama Masanori 
Published: December 2019

AbstractChronic infection withHelicobacter pylori cagA-positive strains is the strongest risk factor for gastric cancer. ThecagAgene product, CagA, is delivered into gastric epithelial cells via the bacterial type IV secretion system. Delivered CagA then undergoes tyrosine phosphorylation at the Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs in its C-terminal region and acts as an oncogenic scaffold protein that physically interacts with multiple host signaling proteins in both tyrosine phosphorylation-dependent and -independent manners. Analysis of CagA using in vitro cultured gastric epithelial cells has indicated that the nonphysiological scaffolding actions of CagA cell-autonomously promote the malignant transformation of the cells by endowing the cells with multiple phenotypic cancer hallmarks: sustained proliferation, evasion of growth suppressors, invasiveness, resistance to cell death, and genomic instability. Transgenic expression of CagA in mice leads to in vivo oncogenic action of CagA without any overt inflammation. The in vivo oncogenic activity of CagA is further potentiated in the presence of chronic inflammation. SinceHelicobacter pyloriinfection triggers a proinflammatory response in host cells, a feedforward stimulation loop that augments the oncogenic actions of CagA and inflammation is created in CagA-injected gastric mucosa. Given thatHelicobacter pyloriis no longer colonized in established gastric cancer lesions, the multistep nature of gastric cancer development should include a “hit-and-run” process of CagA action. Thus, acquisition of genetic and epigenetic alterations that compensate for CagA-directed cancer hallmarks may be required for completion of the “hit-and-run” process of gastric carcinogenesis.
Chemical-genetic profiling reveals limited cross-resistance between antimicrobial peptides with different modes of action
Authors: Kintses Bálint , Jangir Pramod K. , Fekete Gergely , Számel Mónika , Méhi Orsolya , Spohn Réka , Daruka Lejla , Martins Ana , Hosseinnia Ali , Gagarinova Alla , Kim Sunyoung , Phanse Sadhna , Csörgő Bálint , Györkei Ádám , Ari Eszter , Lázár Viktória , Nagy István , Babu Mohan , Pál Csaba , Papp Balázs 
Published: December 2019

AbstractAntimicrobial peptides (AMPs) are key effectors of the innate immune system and promising therapeutic agents. Yet, knowledge on how to design AMPs with minimal cross-resistance to human host-defense peptides remains limited. Here, we systematically assess the resistance determinants ofEscherichia coliagainst 15 different AMPs using chemical-genetics and compare to the cross-resistance spectra of laboratory-evolved AMP-resistant strains. Although generalizations about AMP resistance are common in the literature, we find that AMPs with different physicochemical properties and cellular targets vary considerably in their resistance determinants. As a consequence, cross-resistance is prevalent only between AMPs with similar modes of action. Finally, our screen reveals several genes that shape susceptibility to membrane- and intracellular-targeting AMPs in an antagonistic manner. We anticipate that chemical-genetic approaches could inform future efforts to minimize cross-resistance between therapeutic and human host AMPs.
ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress
Authors: Park Su Hyung , Kang Nalae , Song Eunho , Wie Minwoo , Lee Eun A. , Hwang Sunyoung , Lee Deokjae , Ra Jae Sun , Park In Bae , Park Jieun , Kang Sukhyun , Park Jun Hong , Hohng Sungchul , Lee Kyoo-young , Myung Kyungjae 
Published: December 2019

AbstractMaintaining stability of replication forks is important for genomic integrity. However, it is not clear how replisome proteins contribute to fork stability under replication stress. Here, we report that ATAD5, a PCNA unloader, plays multiple functions at stalled forks including promoting its restart. ATAD5 depletion increases genomic instability upon hydroxyurea treatment in cultured cells and mice. ATAD5 recruits RAD51 to stalled forks in an ATR kinase-dependent manner by hydroxyurea-enhanced protein-protein interactions and timely removes PCNA from stalled forks for RAD51 recruitment. Consistent with the role of RAD51 in fork regression, ATAD5 depletion inhibits slowdown of fork progression and native 5-bromo-2ʹ-deoxyuridine signal induced by hydroxyurea. Single-molecule FRET showed that PCNA itself acts as a mechanical barrier to fork regression. Consequently, DNA breaks required for fork restart are reduced by ATAD5 depletion. Collectively, our results suggest an important role of ATAD5 in maintaining genome integrity during replication stress.
The upper-airway microbiota and loss of asthma control among asthmatic children
Authors: Zhou Yanjiao , Jackson Daniel , Bacharier Leonard B. , Mauger David , Boushey Homer , Castro Mario , Durack Juliana , Huang Yvonne , Lemanske Robert F. , Storch Gregory A. , Weinstock George M. , Wylie Kristine , Covar Ronina , Fitzpatrick Anne M. , Phipatanakul Wanda , Robison Rachel G. , Beigelman Avraham 
Published: December 2019

AbstractThe airway microbiome has an important role in asthma pathophysiology. However, little is known on the relationships between the airway microbiome of asthmatic children, loss of asthma control, and severe exacerbations. Here we report that the microbiota’s dynamic patterns and compositions are related to asthma exacerbations. We collected nasal blow samples (n = 319) longitudinally during a clinical trial at 2 time-points within one year: randomization when asthma is under control, and at time of early loss of asthma control (yellow zone (YZ)). We report that participants whose microbiota was dominated by the commensal Corynebacterium + Dolosigranulum cluster at RD experience the lowest rates of YZs (p = 0.005) and have longer time to develop at least 2 episodes of YZ (p = 0.03). The airway microbiota have changed from randomization to YZ. A switch from the Corynebacterium + Dolosigranulum cluster at randomization to the Moraxella- cluster at YZ poses the highest risk of severe asthma exacerbation (p = 0.04). Corynebacterium’s relative abundance at YZ is inversely associated with severe exacerbation (p = 0.002).
Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
Authors: Thibault Derek , Jensen Paul A. , Wood Stephen , Qabar Christine , Clark Stacie , Shainheit Mara G. , Isberg Ralph R. , van Opijnen Tim 
Published: December 2019

AbstractWhile Tn-Seq is a powerful tool to determine genome-wide bacterial fitness in high-throughput, culturing transposon-mutant libraries in pools can mask community or other complex single-cell phenotypes. Droplet Tn-Seq (dTn-Seq) solves this problem by microfluidics facilitated encapsulation of individual transposon mutants into growth medium-in-oil droplets, thereby enabling isolated growth, free from the influence of the population. Here we describe and validate microfluidic chip design, production, encapsulation, and dTn-Seq sample preparation. We determine that 1–3% of mutants in Streptococcus pneumoniae have a different fitness when grown in isolation and show how dTn-Seq can help identify leads for gene function, including those involved in hyper-competence, processing of alpha-1-acid glycoprotein, sensitivity against the human leukocyte elastase and microcolony formation. Additionally, we show dTn-Seq compatibility with microscopy, FACS and investigations of bacterial cell-to-cell and bacteria-host cell interactions. dTn-Seq reduces costs and retains the advantages of Tn-Seq, while expanding the method’s original applicability.
The role of prefrontal cortex in the control of feature attention in area V4
Authors: Bichot Narcisse P. , Xu Rui , Ghadooshahy Azriel , Williams Michael L. , Desimone Robert 
Published: December 2019

AbstractWhen searching for an object in a cluttered scene, we can use our memory of the target object features to guide our search, and the responses of neurons in multiple cortical visual areas are enhanced when their receptive field contains a stimulus sharing target object features. Here we tested the role of the ventral prearcuate region (VPA) of prefrontal cortex in the control of feature attention in cortical visual area V4. VPA was unilaterally inactivated in monkeys performing a free-viewing visual search for a target stimulus in an array of stimuli, impairing monkeys’ ability to find the target in the array in the affected hemifield, but leaving intact their ability to make saccades to targets presented alone. Simultaneous recordings in V4 revealed that the effects of feature attention on V4 responses were eliminated or greatly reduced while leaving the effects of spatial attention on responses intact. Altogether, the results suggest that feedback from VPA modulates processing in visual cortex during attention to object features.
Predicting quality and quantity of water used by urban households based on tap water service
Authors: Jeandron Aurelie , Cumming Oliver , Kapepula Lumami , Cousens Simon 
Published: December 2019

AbstractDespite significant progress in improving access to safe water globally, inadequate access remains a major public health concern in low- and middle-income countries. We collected data on the bacterial quality of stored drinking water and the quantity of water used domestically from 416 households in Uvira, Democratic Republic of the Congo. An indicator of tap water availability was constructed using invoices from 3685 georeferenced piped water connections. We examined how well this indicator predicts the probability that a household’s stored drinking water is contaminated with Escherichia coli, and the total amount of water used at home daily, accounting for distance from alternative surface water sources. Probability of drinking water contamination is predicted with good discrimination overall, and very good discrimination for poorer households. More than 80% of the households are predicted to store contaminated drinking water in areas closest to the rivers and with the worst tap water service, where river water is also the most likely reported source of drinking water. A model including household composition predicts nearly two-thirds of the variability in the reported quantity of water used daily at home. Households located near surface water and with a poor tap water service indicator are more likely to use water directly at the source. Our results provide valuable information that supports an ongoing large-scale investment in water supply infrastructure in Uvira designed to reduce the high burden of cholera and other diarrhoeal diseases. This approach may be useful in other urban settings with limited water supply access.
Comparison of three models fitting the soil water retention curves in a degraded alpine meadow region
Authors: Pan Tao , Hou Shuai , Liu Yujie , Tan Qinghua 
Published: December 2019

AbstractSoil water retention curve (SWRC) plays an important role in simulating soil water movement and assessing soil water holding capacity and availability. Comparison of fitness between different models to determine the best SWRC model of specific regions is required. In this study, three popular models, van Genuchten, Brooks Corey and Gardner model, were selected for comparing in a degraded alpine meadow region on the eastern Tibetan Plateau. Fitness, error distribution along with key parameters were compared. For each soil horizon, the soil moisture content at all soil water potentials decreased consistently with degradation, thereby integrally moving the SWRCs of all soil depths downward with degradation. The differences in SWRCs across various degradation degrees diminished along with soil depth and soil water potential. The Adj.r2 values of van Genuchten, Brooks Corey and Gardner models ranged in 0.971–0.995, 0.958–0.997, and 0.688–0.909, respectively. The van Genuchten and Brooks Corey models significantly (p < 0.05) outperformed the Gardner model, and have no significant differences in fitness. The fitness of all three models showed no significant changes with degradation. Regardless of degradation degree and soil depth, the fitting error of van Genuchten and Brooks Corey models was mainly distributed in the higher (from –100 hPa to –500 hPa) and lower (below –10000 hPa) potential sections. With regard to the parameters of van Genuchten and Brooks Corey models, the field capacity (θs), and permanent wilting moisture were highly coherent with Adj.r2 values of higher than 0.98, while the curve shape parameter (θr), and air entry pressure of the Brooks Corey model were much lower than those of the van Genuchten model with Adj.r2 values of lower than 0.91. The SWRCs with varying degrees of degradation are best fitted by both van Genuchten and Brooks Corey models but cannot be fitted by Gardner model. Soil water holding capacity decreased with degradation especially in the top soil (0 cm to 30 cm), but the curve shape of all SWRCs did not change significantly with degradation.
Vinculins interaction with talin is essential for mammary epithelial differentiation
Authors: Wang Pengbo , Wu Jian , Wood Amber , Jones Matthew , Pedley Robert , Li Weiping , Ross Robert S. , Ballestrem Christoph , Gilmore Andrew P. , Streuli Charles H. 
Published: December 2019

AbstractVinculin is an essential component of cell adhesion complexes, where it regulates the strength and stability of adhesions. Whilst the role of vinculin in cell motility is well established, it remains unclear how vinculin contributes to other aspects of tissue function. Here we examine the role of vinculin in mammary epithelial cell phenotype. In these cells, correct adhesion to the extracellular matrix is essential for both the formation of polarised secretory acini and for the transcription of tissue-specific milk protein genes. We show that vinculin, through its interaction with talin, controls milk protein gene expression. However, vinculin is not required for the formation of polarised acini. This work reveals new roles for vinculin that are central to cellular differentiation, and for the ability of cells to interpret their extracellular microenvironment.