bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving physical activity in ‘Health Volunteers’: A randomized controlled trial
Authors: Jafarpour Khadijeh , Arastoo Ali Asghar , Gholammnia-Shirvani Zeinab , Montazeri Ali , Haeri-Mehrizi Ali Asghar , Araban Marzieh 
Published: May 2020

Abstract
Background

Health volunteers are women who have humanistic cooperation with healthcare centers to prevent diseases. Health volunteers play an important role in the community. Thus if educated, they could be of great help to health care systems. Since physical inactivity and sedentary lifestyles increase the risk of all cause mortality, the aim of this study was to educate health volunteers on physical activity (PA) applying the theory of planned behavior (TPB).
Methods

This was a randomized controlled trial of an educational intervention to improve physical activity among health volunteers in Shushtar (a southern city), Iran in 2016. A sample of female health volunteers was entered into the study and randomly was assigned to the intervention and the control groups. The intervention included three educational sessions and a walking program. A questionnaire containing items on TPB constructs including: attitude, subjective norms, perceived behavioral control, intention and behavior constructs, the international physical activity questionnaire, and the physiological cost index (PCI- an objective measure of PA) were used to collect data at baseline and 6 months follow-up assessments. Then the data were compared within and between groups using t-test and paried test.
Results

In all 110 ‘Health Volunteers’ (55 per each group) were entered into the study. The mean age of participants was 35.65 ± 10.25 years. The results obtained from analysis showed that educational program improved attitude towards PA, perceived behavioral control, intention, behavior and PCI among the intervention group (P < 0.001). However, no significant changes occurred in the control group in terms of the study variables (P > 0.05). Additionally, comparison of score changes between two groups showed significant differences for all variables except for subjective norms and physical activity behavior (minute per day) group (P > 0.05).
Conclusion

The TPB-directed educational program improved physical activity and physiological cost index. The study framework might be used as a practical template for interventions aimed at improving physical activity among women.
Pathogenic Variants in BRIP1, RAD51C, and RAD51D Identified with Multi-Gene Panel Testing for Hereditary Cancers
Authors: Cummings Shelly , Roman Susana San , Saam Jennifer , Bernhisel Ryan , Brown Krystal , Lancaster Johnathan M. , Usha Lydia 
Published: May 2020

Abstract
Background: Professional society guidelines recommend risk-reducing salpingo-oophorectomy (RRSO) for women with pathogenic variants (PVs) in ovarian cancer-risk genes. Personalization of that intervention is based on gene-specific phenotypes; however, the age of ovarian cancer diagnosis in women with PVs in moderate penetrance ovarian cancer-risk genes is not well characterized. Women who had hereditary cancer panel testing from September 2013-May 2019 were included (N=631,950). Clinical/demographic information was compared for women with a PV in BRIP1, RAD51C, or RAD51D versus in BRCA1 or BRCA2. Results: PVs in BRIP1, RAD51C, or RAD51D were identified in 0.5% of all tested women but in 1.6% of women with a history of ovarian cancer (~3-fold increase). PVs in BRCA1 or BRCA2 were identified in 2.4% of all tested women but in 6.1% of women with a history of ovarian cancer (~2.5-fold increase). The proportion of women with a personal or family history of ovarian cancer was similar among women with a PV in BRIP1, RAD51C, RAD51D, BRCA1, or BRCA2. The median age at ovarian cancer diagnosis was 53 years in BRCA1, 59 years for BRCA2, 65 years for BRIP1, 62 years for RAD51C, and 57 years for RAD51D.Conclusions: These data reinforce the importance of identifying PVs in moderate penetrance ovarian cancer-risk genes. The age at ovarian cancer diagnosis was older for women with PVs in BRIP1, RAD51C, or RAD51D, suggesting that it is safe to delay RRSO until age 45-50 in RAD51D PV carriers and possibly, until age 50-55 in BRIP and RAD51C PV carriers.
Using electrical impedance tomography for rapid determination of starch and soluble sugar contents in Rosa hybrida
Authors: Qian Ji , Zhou Juan , Di Bao , Liu Yang , Zhang Gang 
Published: May 2020

Abstract
Background
Soluble sugar and starch, as carbon sources, directly participate in plant metabolism by providing energy. Conventional determination of plant starch and soluble sugar content usually involves destructive sampling, complicated procedures, and considerable amounts of chemicals and labor. Therefore, there is an urgent need to develop a non-destructive and rapid method for determining plant starch and soluble sugar contents. Electrical impedance tomography (EIT) technology has been used to determine the physiological state and cold resistance of plant tissues. However, so far there have been no reports on the use of EIT for the rapid estimation of soluble sugar and starch contents.
Results
In this study, EIT was used to obtain reconstructed voltage values and estimate starch and soluble sugar contents in the stems of three Rosa hybrida cultivars during February to May. Stems from two of the cultivars were used for establishing regression models for starch and soluble sugar contents as functions of reconstructed voltage values. The third cultivar was used to test the accuracy of the regression models. The results showed that the reconstructed voltage value significantly correlated with soluble sugar and starch contents. The quadratic regression model was best for determining soluble sugar content and the logarithmic regression model was best for determining starch content.
Conclusions
Thus, we preliminarily established and verified regression models for estimating soluble sugar and starch contents using reconstructed voltage values of rose stems. These data provide technical support for using EIT to analyze changes in physiological parameters and to rapidly estimate physiological indexes of plants.
miR-577 suppressed the metastasis, EMT and viability via NF-κB pathway by targeting CXCL5 through in hepatocellular carcinoma
Authors: Tu Lirui , Liu Jing , Li Wei , Song Xiuguang , Xu Hongwei 
Published: May 2020

Abstract
Background

Hepatocellular carcinoma (HCC) is a common malignant cancer worldwide. miR-577 have a role in inhibiting cell viability, metastasis in many tumors. This research was to explore the great role of miR-577 in hepatocellular carcinoma.
Methods

RT-qPCR and western blot were performed to evaluate the the miR-577 and genes mRNA and protein levels. Transwell assay and CCK-8 were applied to measure the viable and invasive abilities. Meanwhile, Kaplan-Meier method was used to assess the survival of HCC patients.
Results

miR-577 was downregulated in HCC tissues, which predicted a worse overall survival in HCC. miR-577 targeted to CXCL5 and mediated its expression in HCC. miR-577 suppressed cell invasion and EMT in HuH-7 cells. miR-577 inhibited cell viability via NF-κB pathway. In addition, miR-577 overxpression impaired the xenograft growth of HuH-7 cells.
Conclusion

miR-577 inhibited cell invasion, EMT and viability via NF-κB pathway by targeting to CXCL5 in HCC. The newly identified miR-577/CXCL5 axis provides novel insight into the pathogenesis of hepatocellular carcinoma.
Novel strategy with the automatic non-coplanar volumetric-modulated arc therapy for angiosarcoma of the scalp.
Authors: Inui Shoki , Ueda Yoshihiro , Ohira Shingo , Tsuru Haruhi , Isono Masaru , Miyazaki Masayoshi , Koizumi Masahiko , Teshima Teruki 
Published: May 2020

Abstract
Background Total scalp irradiation presents technical and dosimetric challenges. While reports suggest that HyperArc, a new stereotactic radiosurgery planning technique, is associated with high conformity and rapid dose fall-off, the performance of HyperArc plans for cancers of the head and neck regions has not been explored. The current study aimed to compare the dosimetric performance of HyperArc plans with those of non-coplanar volumetric-modulated arc therapy (VMAT) plans in angiosarcoma of the scalp.Methods Six patients with angiosarcoma of the scalp were included in this study. Performance of three different non-coplanar plans administered using TrueBeam Edge was compared. Three plans were employed namely VMAT using flattening filter (FF) beams (VMAT-FF), HyperArc using FF beams (HyperArc-FF), and HyperArc using flattening filter free (FFF) beams (HyperArc-FFF). The dose distribution, dosimetric parameters, and dosimetric accuracy for all plans were evaluated.Results The three plans showed no statistically significant differences in target volume coverage, conformity, and homogeneity. With regard to the normal brain tissue, the received volume doses were significantly lower for the HyperArc-FF and the HyperArc-FFF plans than for the VMAT-FF plans. Mean brain doses were 17.56 ± 5.70 Gy, 12.88 ± 3.36 Gy, and 13.24 ± 3.55 Gy in the VMAT-FF, the HyperArc-FF, and the HyperArc-FFF plans, respectively. There were almost no differences in sparing the organs at risk between the HyperArc-FF and HyperArc-FFF plans. The HyperArc-FF and HyperArc-FFF plans provide a shorter beam-on time than does the VMAT-FF plan. The 3%/2 mm gamma test pass rates were above 95% for all plans.Conclusions Our results suggested that the HyperArc plan can be potentially used for radiation therapy of target regions with large and complicated shape, such as the scalp, and that there are no advantages of using FFF beams.
Advantage of Laparoscopy Surgery for Elderly Colorectal Cancer Patients without Compromising Oncologic Outcome
Authors: Chern Yih Jong , Hung Hsin-Yuan , You Jeng-Fu , Hsu Yu-Jen , Chiang Jy-Ming , Hsieh Pao-Shiu , Tsai Wen-Sy 
Published: May 2020

Abstract
Backgrounds: Laparoscopic surgery has achieved good results in elderly patients with colorectal cancer (CRC). In this study, we compared the short-term and long-term outcomes of open surgery and laparoscopic surgery in CRC patients aged above 75 years at a single tertiary medical center.Methods: Between January 2009 and December 2015, we studied 967 patients who underwent curative resection for primary colorectal adenocarcinoma without distant metastasis in a single institution. Of the enrolled subjects, 305 underwent laparoscopic surgery, and 662 received open laparotomy surgery.Results: Compared to the patients who received open surgery, those received laparoscopic surgery had prominent shorter postoperative stay (10.3 vs. 13.5 days p < 0.001) and similar postoperative mortality (p = 0.082) and morbidity (p = 0.354). In the laparoscopy cohort, 6 of 305 patients were converted to open surgery and 1 died. The long-term overall survival, cancer-specific survival and recurrence rate were all similar between both cohorts in each stage.Conclusions: Laparoscopic surgery is suitable for elderly patients due to shorter postoperative stay, similar long-term outcomes with open surgery and acceptably low conversion rates. For long-term overall and oncological outcome, the results of laparoscopic surgery were similar to that of open surgery in each TNM stage.
Comparitive Study Of Short-Term Outcomes Of Preterm Babies ≤30 Weeks In A Tertiary Care Hospital Between India And The U.K
Authors: diggikar shivshankar , Nagesh Karthik , Kumar Arun , Aladangady Narendra 
Published: May 2020

Abstract
Large numbers of preterm babies are born in middle income countries and neonatal care is improving in these countries. However, comparative clinical outcomes of preterm babies between a tertiary neonatal unit from middle income country and higher income country is limited. Objective: To compare the clinical profile and short-term outcomes of preterm babies ≤ 30 weeks gestation admitted to a tertiary care hospital in India and the U.K. Design: Retrospective cohort observational study using anonymised data from Electronic Patient Records. Setting: Regional tertiary neonatal intensive care unit (NICU) of Manipal Hospitals, Bangalore, India and Homerton University Hospital, London, UK. Participants: Preterm babies born at ≤ 30 weeks gestation admitted to NICUs over five year period (January 2011 to December 2015). Infants with major congenital abnormalities were excluded. Intervention: This is an observational study and routine care was provided as per departmental standard protocols. Main outcome measures: Comparison of neonatal unit mortality between two centres, infant morbidities till discharge from neonatal unit. Results: A total of 740 babies from the U.K centre and 294 babies from Indian centre were enrolled into the study. The mean gestational age in the U.K cohort was 26.6 ± 2.16 weeks, whereas in Indian cohort was 28.4 ± 1.66 weeks (p,<0.001). A significantly lower number of mothers received antenatal steroid (37% v 73%), and higher number of babies were inborn (78.6%) in Indian centre compared to the UK centre (67.3%; p < 0.001). Incidence of BPD and IVH ≥ grade 2 was significantly lower, and ROP needing treatment was significantly higher in Indian centre. Higher number of babies were treated for ductus arteriosus and incidence of culture positive sepsis was significantly high in Indian cohort babies. Survival of babies born at gestational age > 28 weeks was comparable between the U.K and Indian centres. Survival of babies born at ≤ 28 weeks gestation was lower in Indian centre. Conclusions and relevance: The survival of babies above 28 weeks prematurity was comparable between a tertiary care centre in India and the U.K. However, survival of babies ≤ 28 weeks gestation was lower in Indian centre. Incidence of ROP and sepsis was higher in Indian centre.
Oral Bisphenol-A Regulates The Development and Function of Reproductive System through Differential Expression of METTL3
Authors: Zhang Zhe , Wang Yong , Zhu Yuan , Zhang Zhifan , Wang Daojuan , Wang Hongwei , Yan Guijun 
Published: May 2020

Abstract
Endocrine-disrupting chemicals (EDCs), which have a profound impact on the reproductive system, can cause endocrine and reproductive disorders, such as polycystic ovary syndrome (PCOS). Bisphenol-A (BPA) is a common endocrine disruptor which can affect the function of the reproductive system. However, the mechanism by which this molecule disrupts normal reproduction is still unclear. In this study, we hypothesized that oral BPA would have a effect on the structure and function of the reproductive system in adolescent female rats, and we would explore a kind of feasible mechanism for this effect. 4-week-old Sprague-Dawley (SD) rats were intragastrically treated for 10 weeks, which were divided into blank group (n = 8), control group (soybean oil, n = 8), three BPA-treatment groups (0.5 mg/kg BPA + soybean oil, 5 mg/kg BPA + soybean oil, 50 mg/kg BPA + soybean oil, n = 8). The results showed that 0.5 mg/kg oral BPA increased the coefficient of uteri, and oral BPA increased the length of uteri in rats without causing hyperandrogenism and ovarian polycystic changes. Oral BPA disturbed the expression of CYP17A1, CYP11A1 and METTL3 in ovaries. Our results suggested that oral BPA might partially interfere uterine morphology and the level of androgen synthetases with RNA methylation by disturbing the expression level of METTL3. RNA methylation might be a new way to explain the interference mechanism of BPA.
In silico screening of known small molecules to bind ACE2 specific RBD on&nbsp;Spike Glycoprotein of SARS-CoV-2 for repurposing against COVID-19
Authors: R Bharath B , Damle Hrishikesh , Ganju Shiban , LathaDamle  
Published: May 2020

Abstract
Human coronavirus (SARS-CoV-2) is causing a pandemic with significant morbidity and mortality. Although, no effective novel drugs are available, drug repurposing is emerging as an effective strategy. In this study, we present an in silico drug repurposing study implementing successful concepts of computer aided drug design (CADD) technology with an objective to repurpose known drugs to interfere the viral cellular entry via the spike glycoprotein (SARS-CoV-2-S), which mediates the virus–cell receptor interaction. SARS-CoV-2-S uses ACE2 to enter cells. Totally, 4015 known and approved small molecules were screened for interaction with SARS-CoV-2 S through docking studies and 15 lead molecules were shortlisted. Further, three molecules streptomycin, ciprofloxacin and Glycyrrhizic acid (GA) were selected based on their reported anti-viral activity, safety, availability, affordability and subjected for Molecular Dynamics (MD) simulation. The MD simulation results indicate that GA from plant origin may be repurposed against SARS-CoV-2 and further studies are needed for validation.
Development of Fluorescence/MR Dual-Modal Manganese-Nitrogen-Doped Carbon Nanosheets as An Efficient Contrast Agent for Targeted Ovarian Carcinoma Imaging
Authors: Han Cuiping , Xie Ting , Wang Keying , Jin Shang , Li Ke , Dou Peipei , Yu Nana , Xu Kai 
Published: May 2020

Abstract
Background: Development of sensitive and specific imaging approaches for the detection of ovarian cancer holds great promise for improving the therapeutic efficacy and the lifespan of the patients. Results: In this study, manganese-nitrogen doped carbon nanosheets coupled with Anti-HE4 monoclonal antibody (Mn-N-CNSs@Anti-HE4) were synthesized for the specific and targeted fluorescence/MR dual-modal imaging of ovarian carcinoma. The prepared Mn-N-CNSs@Anti-HE4 nanoprobe revealed excellent aqueous dispersity, good colloidal stability, great optical properties and high longtudinal relaxivity rate (r1 =10.30 mM-1s-1). Encouraged by the tunable photoluminiscence of the nanoprobe, the ovarian carcinoma cells were specifically labeled by the Mn-N-CNSs@Anti-HE4 nanoprobe with multi-color fluorescences (blue, green, red). Benefiting from the high r1 relaxivity, the nanoprobe exhibited targeted and enhanced MR contrast effect in the ovarian carcinoma cells and tumor bearing mice model. Besides, the high biocompatibility and easy excretion from the body of the nanoprobe were further confirmed in vivo. Conclusion: The prepared Mn-N-CNSs@Anti-HE4 with excellent biocompatibility, high-performance and superior tumor-targeting ability provides a novel fluorescence/MR dual-modal nanoprobe for specific labeling and detection of ovarian carcinoma cells in vitro and in vivo.
Degradation and biodegradability improvement of the landfill leachate using electrocoagulation with iron and aluminum electrodes: A comparative study
Authors: Bharath M. , Krishna B. M. , Manoj Kumar B. 
Published: May 2020

Abstract
This present study investigates the comparative study of iron and aluminum electrodes for the treatment of landfill leachate by the Batch Electrocoagulation (EC) technique. The performance of EC was used to determine the removal efficiency of COD and Color. The effects of operating conditions such as electrode material, stirring speed, inter-electrode distance, electrolysis time, initial pH, and applied voltage were studied to evaluate the performance of the electrode. The electrodes were arranged in a monopolar mode by applying different cell voltages of 4, 6, 8, 10 and 12 V for 180 min of electrolysis time (ET) with a varying inter-electrode distance between 1 and 4 cm. The iron and aluminum electrodes can be successfully used as anodes and cathodes for the treatment process, which makes the process more efficient and easier to maintain. Based on the obtained results, it was observed that there was an increase in BOD/COD ratio from 0.11 to 0.79. The maximum removal of COD and Color was found to be 76.5% and 67.2% respectively, accomplished with 105 min optimum electrolysis time with a pH of 9.25 using an iron electrode. In the case of the aluminum electrode, the BOD/COD ratio was increased from 0.11 to 0.66. Over 78.4% of COD and 77.0% of Color removal was obtained with 90 min optimum electrolysis duration and pH 9.3 with an optimum 10 V and an optimum inter-electrode distance of 1 cm. However, the aluminum electrode is superior to iron as a sacrificial electrode material in terms of Color and COD removal efficiency. The aluminum electrode significantly treated landfill leachate by the electrocoagulation method under optimum experimental conditions.
In Vitro Data of Current Therapies for SARS-CoV-2
Authors: Anastasiou Ioanna A. , Eleftheriadou Ioanna , Tentolouris Anastasios , Tsilingiris Dimitrios , Tentolouris Nikolaos 
Published: May 2020

Background:
In December 2019, a new coronavirus, named Severe Acute Respiratory
Syndrome Coronavirus 2 (SARS-CoV-2), emerged from China, causing pneumonia
outbreaks first in the Wuhan region and then spread worldwide. Due to a lack of efficient and
specific treatments and the need to contain the epidemic, drug repurposing appears to be the
most efficient tool to find a therapeutic solution.

Objectives:
The aim of this study was to summarize in vitro data of current agents used for the
management of SARS-CoV-2 all over the world.

Methods:
A literature search of articles from January 2000 until April 2020 was performed
using MEDLINE, EMBASE and the Cochrane Library to assess in vitro data of current or putative
therapies for SARS-CoV-2.

Results:
Although in vitro studies are scarce, data regarding chloroquine, hydroxychloroquine,
remdesivir, nitazoxanide, teicoplanin, ivermectin, lopinavir, homoharringtonine, and
emetine seem promising.

Conclusion:
Scientists all over the world should work together and increase their efforts in
order to find feasible and efficient solutions against this new global viral threat.
Nano-sized Metal and Metal Oxide Modified Electrodes for Pharmaceuticals Analysis
Authors: Topal Burcu Dogan , Sener Ceren Elif , Kaya Basak , Ozkan Sibel Aysıl 
Published: May 2020

:
The electrochemical analysis offers a number of important advantages such as providing
information on pharmaceuticals analysis and their in vivo redox processes and pharmacological activity.
The interest in developing electrochemical sensing devices for use in clinical assays is growing rapidly.
Metallic nanoparticles can be synthesized and modified with various chemical functional groups,
which allow them to be conjugated with antibodies, ligands, and drugs of interest.

:
In this article, the novel developments to enhance the performance of sensor modified with metal nanoparticles
of pharmaceuticals were reviewed. A discussion of the properties of metal nanostructures
and their application in drug analysis is presented. Their application as a modifier agent in determining
low levels of drugs in pharmaceutical dosage forms and biological samples is discussed. It has been
found that the electrocatalytic effect of the electrode, sensitivity and selectivity were increased using
various working electrodes modified with nano-sized metal, metal oxide and metal/metal oxide
particles.