bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cochlear Implant Rehabilitation During Covid-19 Pandemic:&nbsp;A Parents’ Perspective
Authors: Sahoo Lokanath , Kumari Abha , Patnaik Uma , Dwivedi Gunjan 
Published: September 2020

Abstract
Aim and Objectives: The current study was undertaken to assess the effect of Coronavirus disease 2019 (COVID-19) pandemic on cochlear implant rehabilitation of children with congenital non syndromic bilateral severe to profound sensorineural hearing loss (SNHL). The objectives were to find out the psychological impact of COVID-19 on parents of children with cochlear implant habilitation, to assess the performance of children with cochlear implant habilitation through tele therapy and to analyze the problems faced by the parents to obtain the professional hearing health care services for their cochlear implanted children.Material and Methods: An online questionnaire survey was conducted amongst the parents of children who had undergone cochlear implantation (CI) consequent to congenital severe to profound SNHL. Prior to COVID-19 pandemic, these children received regular auditory verbal therapy (AVT) including visits to the Centre for audiological services (mapping and troubleshooting of the speech processor).Results: The online questionnaire survey, undertaken by a total of fifty (50) parents was analyzed. All the parents unanimously reported that COVID-19 pandemic has adversely affected access to the professional health care services for regular mapping and troubleshooting of the speech processor and thus their children’s rehabilitation. However, active involvement between the professional health care services and the parents through video consultation and tele auditory verbal therapy has definitely helped the children to a great extent.Conclusion: The present study emphasises the great challenge posed by the COVID-19 pandemic for continued rehabilitation of CI children. To overcome this situation, an innovative digital media to address such medical issues through tele medicine, tele audiology and tele therapy is warranted.
EFEITO DO BIOATIVADOR DE SOLO NO DESENVOLVIMENTO INICIAL DO CAFEEIRO
Authors: Souza Eduardo Martins de , Junior Kleso Silva Franco , Brigante Giselle Prado , Brockelmann Carlos Eduardo , Tavares Renata Mendes , Dias Márcio de Souza , Marques Douglas José , Carneiro João Paulo Costa 
Published: September 2020

A cafeicultura tem grande importância econômica no território nacional, entretanto, enfrenta algumas limitações no seu manejo, principalmente na sua implantação, pelo fato de comprometer todo o processo produtivo. A utilização de bioativadores de microrganismos no solo na instalação de lavouras cafeeiras gera um impacto de reciclagem de nutrientes auxiliando no aproveitamento, desenvolvimento e absorção da lavoura cafeeira. Os bioestimulantes à base de algas marinhas, enzimas e microrganismo solo, vêm sendo utilizados como uma alternativa para nutrição de plantas, pelo fato da intensa demanda de nutrientes no sistema atual de agricultura. Neste estudo, objetivou-se verificar o efeito de diferentes dosagens de bioativador de solo (Vitasoil®) na implantação de lavoura cafeeira (Catuai 144 vermelho). Foram testados uma aplicação via solo em 5 doses de 4, 6, 8,10 e 12 gramas por planta, somada a 3 aplicações de 3 gramas via foliar na mesma, para fazer a utilização da mesma ocorreu a hidratação com 100 ml de água para cada grama de produto. Aos 120 dias foram realizadas avaliações no experimento, medições da parte aérea e radicular, contagem de folhas, espessura do caule, pesagem da fração aérea e radicular verde e seca, área foliar e relação parte aérea /raiz. O delineamento experimental foi em blocos ao acaso. Entre os dados analisados observou que o uso do bioativador se mostrou superior estatisticamente quando comparado à testemunha, não diferindo entre si em dosagens. O ativador de microbiota de solo (Vitasoil®) aplicado via solo e foliar em mudas de café, promoveu um melhor desenvolvimento inicial do cafeeiro sendo observado em diâmetro, peso verde e seco, número de folhas por muda, área foliar, altura de planta, comprimento de raiz e relação parte aérea/raiz.
Acetogenin Extracted from Annona muricata Prevented the Actions of EGF in PA-1 Ovarian Cancer Cells
Authors: Periyasamy Loganayaki , Muruganantham Bharathi , Deivasigamani Malarvizhi , Lakshmanan Hariprasath , Muthusami Sridhar 
Published: September 2020

Background:In individuals with ovarian cancer, an increase in the circulating level of the epidermal growth factor (EGF) is readily apparent. Ovarian cancer cells exhibit signaling pathway of the epidermal growth factor (EGFR) and respond to the EGF. Annona muricata (AM) has been shown to decrease ovarian cell proliferation however, role of AM in regulating EGF actions is not yet to be reported.Objective:In this study, we proposed that the fractionated compound acetogenin can inhibit the activation of EGFR-regulated signaling cascades such as MAPK7 / PI3K-Akt / mTOR / STAT upon EGF stimulation.Methods:Ethanolic extract was prepared for the whole AM plant and Thin Layer Chromatography (TLC) was performed to characterize the secondary metabolites and each fraction was assessed using kedde reagent for the presence of acetogenin. The effects of acetogenins were then tested on the survival of PA-1 ovarian cancer cells under basal and EGF stimulated conditions. To delineate the role of acetogenin in EGFR signaling cascades, the in silico docking studies were conducted.Results:The fraction of acetogenin decreased the viability of EGF induced PA-1 ovarian cancer cells that indicating the EGF inhibitory effects of acetogenin. The docking studies specifically illustrated that when the acetogenin binding with tyrosine kinase (TK) and regulatory unit (RU) which subsequently resulted in a reduction in EGF induced the survival of PA-1 ovarian cancer cells.Discussion:The vital regulatory role of acetogenin reported in this study indicate significant anticancer activities of acetogenin from AM. The in silico study of the acetogenin function predicted that it binds specifically to Asp837 (phosphor-acceptor site) of EGFR, essential for phosphorylation of substrates in the TK domain and RU which promote downstream signaling.Conclusion:Acetogenin isolated from AM effectively inhibited the survival of PA-1 ovarian cancer cells through impaired EGF signaling.
Expression of Rab3b in Human Glioma: Influence on Cell Proliferation and Apoptosis
Authors: Luo Qili , Liu Yueping , Yuan Zilin , Huang Lvshuai , Diao Bo 
Published: September 2020

Background :
Glioma is the most common human central nervous system tumour with a high degree
of malignancy. Some Rab GTPases have significant effects on glioma.

Objective:
This study aimed to investigate the effect of Rab3b (Rab GTPase3b) on human glioma cell proliferation
and apoptosis by silencing Rab3b and to initially verify the value of Rab3b expression for the diagnosis
and progression in human glioma.

Methods:
Rab3b was silenced by siRNA transfection. Human glioma tissues and normal brain tissues adjacent
to glioma were obtained by surgery. Rab3b, P53, Caspase 7, Bax, and Bim mRNA and protein expression levels
were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. Cell
proliferation was detected by the cell counting kit-8 assay, and the cell cycle and apoptosis were analysed using
flow cytometry.

Results:
Rab3b mRNA and protein expression in human glioma U251 and U87 cells were significantly downregulated
after Rab3b silencing. Rab3b silencing inhibited glioma cell proliferation by promoting cell cycle arrest
and induced apoptosis by upregulating the expression of apoptosis-related proteins. Rab3b expression in human
glioma (n = 33) was significantly higher than that in normal brain tissues adjacent to glioma (n = 15). In addition,
Rab3b expression levels in high-grade gliomas (WHO III-IV, n = 19) were also significantly higher than
those in low-grade gliomas (WHO I-II, n = 14).

Conclusion:
Rab3b expression levels are significantly related to the progression of gliomas. Moreover, Rab3b
silencing not only significantly inhibits cell proliferation in gliomas via cell cycle arrest but also promotes cell
apoptosis by upregulating the expression levels of apoptosis-related proteins; however these preliminary in
vitro results warrant validation on in vivo studies.
Oncogenic LncRNA CASC9 in Cancer Progression
Authors: Qi Yuying , Song Chaoying , Zhang Jiali , Guo Chong , Yuan Chengfu 
Published: September 2020

Background:
Long non-coding RNAs (LncRNAs), with the length of over 200 nucleotides, that originate
from intergenic, antisense, or promoter-proximal regions, are a large family of RNAs that lack coding capacity.
Emerging evidences illustrated that LncRNAs played significant roles in a variety of cellular functions
and biological processes in profuse human diseases, especially in cancers. Cancer susceptibility candidate 9
(CASC9), as a member of the LncRNAs group, firstly found its oncogenic function in esophageal cancer. In the
following recent studies, a growing amount of human malignancies are verified to be correlated with CASC9,
most of which are derived from the squamous epithelium tissue. This present review attempts to highlight the
latest insights into the expression, functional roles, and molecular mechanisms of CASC9 in different human
malignancies.

Methods:
In this review, the latest findings related to the pathophysiological processes of CASC9 in human cancers
were summarized and analyzed, and the associated studies collected in systematic retrieval of PubMed
used lncRNA and CASA9 as keywords.

Results:
CASC9 expression is identified to be aberrantly elevated in a variety of malignancies. The over-expression
of CASC9 has been suggested to accelerate cell proliferation, migration, cell growth and drug resistance of
cancer cells, while depressing cell apoptosis, revealing its role as an oncogene. Moreover, the current review demonstrated
CASC9 as closely related to the neoplastic transformation of squamous epithelial cells and squamous
metaplasia in non-squamous epithelial tissues. Finally, we discuss the limitations and tremendous diagnostic/
therapeutic potential of CASC9 in various human cancers.

Conclusion:
Long non-coding RNA CASC9 likely serve as useful disease biomarkers or therapeutic targets
which be effectively applied in the treatment of different kinds of cancers.
Expression and Prognostic Significance of the MMP Family Molecules in Bladder Cancer
Authors: Shen Chong , Da La , Wu Zhouliang , Wang Yujie , Gao Shen , Tian Dawei , Hu Hailong 
Published: September 2020

Background:
Bladder cancer (BC) is the 10th most common cancer worldwide with significantly varied prognosis in different pathological subtypes. MMPs, a group of enzymes, could involve in the invasion and metastasis of numerous
malignancies. The function of MMPs in BC is partly reported in several studies but with a great conflicts; hence, a systematic analysis of expression levels and prognostic values of these MMP genes are still to be determined.

Methods:
Firstly, differentially expressed genes (DEGs) of MMPs were identified in ONCOMINE, GEPIA and UALCAN
databases; and these DEGs were also detected by real-time RT-qPCR. More importantly, we investigated the clinical significance of these DEGs in BC patients via Kaplan-Meier (KM) Plotter, UALCAN and cBioPortal databases.

Results:
The study found that mRNA expression of MMP1/11 in BC samples was significantly higher than that in normal
bladder tissues, and MMP2/3 were lower in the former than in the latter. The expression level of MMP1/2/7/9/11/13/23B
were significantly related to the tumour stages. Furthermore, the prognostic analysis suggested that the high transcription
levels of MMP7 and low transcription levels of MMP23A were correlated with favorable relapse-free survival and overall
survival in the patients with BC, respectively. Notably, high MMP11/13 expression levels indicated poor overall survival
(OS) and relapse-free survival (RFS) in patients with BC.

Conclusion:
This study revealed that MMP1/2/3/7/9/11/13/23A/23B are possible prognostic biomarkers and clinical therapeutic targets for patients with BC.
miRNA-146a Improves Immunomodulatory Effects of MSC-derived Exosomes in Rheumatoid Arthritis
Authors: Tavasolian Fataneh , Hosseini Ahmad Zavaran , Soudi Sara , Naderi Mahmood 
Published: September 2020

Background:
Rheumatoid arthritis (RA) is a severe inflammatory joint disorder, and several
studies have taken note of the probability that microRNAs (miRNAs) play an important role in
RA pathogenesis. MiR-146 and miR-155 arose as primary immune response regulators. Mesenchymal
stem cells (MSCs) immunomodulatory function is primarily regulated by paracrine factors,
such as exosomes. Exosomes, which serve as carriers of genetic information in cell-to-cell communication,
transmit miRNAs between cells and have been studied as vehicles for the delivery of therapeutic
molecules.

Aims:
The current research aimed to investigate the therapeutic effect of miR-146a/miR-155 transduced
mesenchymal stem cells (MSC)-derived exosomes on the immune response.

Methods:
Here, exosomes were extracted from normal MSCs with over-expressed
miR-146a/miR-155; Splenocytes were isolated from collagen-induced arthritis (CIA) and control
mice. Expression levels miR-146a and miR-155 were then monitored. Flow cytometry was performed
to assess the impact of the exosomes on regulatory T-cell (Treg) levels. Expression of some
key autoimmune response genes and their protein products, including retinoic acid-related orphan
receptor (ROR)-γt, tumor necrosis factor (TNF)-α, interleukin (IL)-17, -6, -10, and transforming
growth factor (TGF)-β in the Splenocytes was determined using both quantitative real-time PCR
and ELISA. The results showed that miR-146a was mainly down-regulated in CIA mice. Treatment
with MSC-derived exosomes and miR-146a/miR-155-transduced MSC-derived exosomes significantly
altered the CIA mice Treg cell levels compared to in control mice.

Results:
Ultimately, such modulation may promote the recovery of appropriate T-cell responses in
inflammatory situations such as RA.

Conclusion:
miR-146a-transduced MSC-derived exosomes also increased forkhead box P3 (Fox-
P3), TGFβ and IL-10 gene expression in the CIA mice; miR-155 further increased the gene expressions
of RORγt, IL-17, and IL-6 in these mice. Based on the findings here, Exosomes appears to
promote the direct intracellular transfer of miRNAs between cells and to represent a possible therapeutic
strategy for RA. The manipulation of MSC-derived exosomes with anti-inflammatory miRNA
may increase Treg cell populations and anti-inflammatory cytokines.
Transmissibility and Epidemicity of COVID-19 in India: A Case Study
Authors: Jinila Bevish , Shyry Prayla S. 
Published: September 2020

Background:
The global outbreak of COVID-19 was first reported in
Wuhan, China, in the late 2019. The origin of the infection is still unclear. Over
time, it spread to 150 countries in the world, due to which, the World Health Organization
(WHO) declared the infectious disease to be pandemic.

Objective:
COVID-19 transmitted to India due to the arrival of travelers from
other countries. The transmissibility and epidemicity of COVID-19 in India are
exponential. So, in order to understand the above characteristics, specifically
COVID-19 status in India has to be analyzed. To gain better insight on the
matter, the state of Kerala is selected. The epidemiological characteristics of patients
in Kerala, South India, and the possible transmission of COVID-19 from
asymptomatic members to other peers are shown describing certain cases.

Methodology:
The COVID-19 dataset is taken from the Kaggle dataset. This dataset
contains the details of the infected patients from different states of India.
Statistical analysis techniques were used to analyze the distribution of the affected
cases in a particular state.

Case Presentation:
The analysis shows that there is a possibility of the transmission
of infection even during the incubation period. The recent trend in the number
of infected cases in India is discussed.

Conclusion:
The transmissibility of COVID-19 and its epidemicity in India are
discussed. Specifically, a case study on COVID-19 cases in the state of Kerala relating
transmissibility is also summarized. Further, data related to patents on coronavirus
is also discussed. From the analysis, it can be concluded that there is a
possibility of COVID-19 transmission even during the incubation period. The
preventive measures to overcome COVID-19 and methods to increase immunity
are discussed.
Integration of Fuzzy Logic and ABC Algorithm for Optimized Network Selection in Heterogeneous Wireless Environment
Authors: Litake Shilpa R. , Mukherji Prachi 
Published: September 2020

Background & Objective:
Various Radio Access Technologies are integrated in the next
generation of Heterogeneous Wireless Networks. The coexistence of various kinds of wireless access
networks ensures high service quality (QoS) for the users. Seamless vertical handover plays a significant
role in providing ubiquitous access to users. The ability to select the optimal access network out of available
access networks decides the comprehensive performance of the system. A novel scheme: Handoff Urgency
Estimator and Target Access Network Selector using Artificial Bee Colony algorithm (HUETANSABC)
for deciding the necessity of handover and selection of the best network is proposed in this paper.
The objective of the proposed work is to choose the most promising access network out of available coexisting
networks for enhancing user experience. Fuzzy logic provides reliable results even when the input
parameters are random in nature and can not be defined precisely. Artificial Bee Colony is an effective
method for searching and optimization. Proposed system combines best of fuzzy logic and ABC algorithm
for timely initiation of vertical handover. To gather the required information for handover, services provided
by IEEE 802.21 standard are utilized. Proposed integration of fuzzy logic and the ABC algorithm has
resulted in a decreasing number of unnecessary handovers.

Methods:
The effect of varying context parameters is analyzed using Fuzzy Inference System to estimate
the urgency of handover. The optimization of a target access network selection process is
achieved using meta-heuristic method.

Results:
Simulation results on MATLAB indicate that the proposed system performs better than
ABC and Multiple Attribute Decision Making (MADM) techniques.
COVID-19: An Insight on the Third Respiratory Global Emergency of the Century
Authors: Batra Sonali , Sharma Sumit , Verma Payal , Arora Nikhil 
Published: September 2020

Introduction:
COVID-19 presents a global emergency in recent days and as a consequence,
the whole world is in a state of war. Continuous efforts by researchers are being made to establish a
sound scientific understanding regarding the behavior of the virus and its pathogenesis.

Background:
In recent days, a lot of scientific data are published describing viral transfusion into host
cells, compared with SARS-CoV-1, viability of SARS-CoV-2, fatality and diagnosis of infection among
infected patients, thereby, motivating to make right decisions in order to treat and mitigate COVID-19.

Review Results:
Coronaviruses usually affect the respiratory tract and produce symptoms similar to
pneumonia with varied complications. The spike protein on the surface of the virus makes the SARSCoV-
2 phylogenetically different from other viruses of the corona family. SARS-CoV-2 has an affinity
to angiotensin converting enzyme-2 receptors present on human cells. The fatality of the disease is
found to be low, but the severity of the disease might vary from person to person. The incubation period
is 14 days but the symptoms like dry cough and high fever are evident around 3-7 days. Already known
anti-inflammatory immunomodulators and antiviral drugs are being tested and still under clinical trials.

Conclusion:
There are many current strategies which are being tried and practiced to overcome
this pandemic disease apart from precautionary measures. Although now, some decline has been
seen, but the question still remains the same of whether the scientists are approaching towards the clinical
solution, or are still in the midstream. This requires more study and intensive research to finally
come to a concrete conclusion.
Expressions of Individualization on the Internet and Social Media: Multigenerational Focus Group Study
Authors: Mayer Gwendolyn , Alvarez Simone , Gronewold Nadine , Schultz Jobst-Hendrik 
Published: September 2020

Background
Growing individualization within the past decades has been described as a fundamental shift in society. Studies have reported how the digital age promotes new forms of individualism with self-tracking technologies and self-presentation in social networks. Potential harmful effects on the mental health of young adults have already been at the forefront of research. However, 2 questions that remain unanswered are how emotional experiences and expressions of self-relatedness differ among generations in their usage of the internet and social media, and if an increasing individualism can be observed by this.

Objective
The aim of this study is to examine whether the use of the internet and social media has led people to be more concerned about themselves than former generations. The potential consequences of mental and emotional distress among different age groups are analyzed.

Methods
A focus-group approach was chosen to study the following age groups: Baby Boomers (those born in 1950-1965), Generation X (those born in 1966-1980), and Digital Natives (those born in 1981-2000). We organized 6 focus groups with 36 participants who discussed their private usage of the internet and social media, different devices, platforms and functions, communication behavior, and self-tracking. We applied inductive category formation and followed the Standards for Reporting Qualitative Research (SRQR) checklist.

Results
We found differences in the 3 studied generations regarding the reasons for their use of the internet and social media, the effects of this use, personal feelings and experiences, expressions of self-relatedness, and social relationships. The Baby Boomers voiced a wish to stay autonomous while being in contact with their personal network. Generation X included enthusiastic members who appreciate self-tracking for curiosity and fascination, as well as people who felt fears about data surveillance. The Digital Natives reported a wish to optimize their own body by self-tracking while being faced with norms and expectations that were communicated via the internet and social media.

Conclusions
All generations expressed self-relatedness, yet by different means. The Baby Boomers expressed less individualism than Generation X and the Digital Natives, who felt the highest strain due to social comparisons. However, all generations reported specific, potentially problematic consequences for their mental health. Age-specific coping strategies are necessary to promote a mentally healthy way of using the internet and social media.