bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure and function of minor pilins of type IV pili
Authors: Jacobsen Theis , Bardiaux Benjamin , Francetic Olivera , Izadi-Pruneyre Nadia , Nilges Michael 
Published: November 2019

AbstractType IV pili are versatile and highly flexible fibers formed on the surface of many Gram-negative and Gram-positive bacteria. Virulence and infection rate of several pathogenic bacteria, such as Neisseria meningitidis and Pseudomonas aeruginosa, are strongly dependent on the presence of pili as they facilitate the adhesion of the bacteria to the host cell. Disruption of the interactions between the pili and the host cells by targeting proteins involved in this interaction could, therefore, be a treatment strategy. A type IV pilus is primarily composed of multiple copies of protein subunits called major pilins. Additional proteins, called minor pilins, are present in lower abundance, but are essential for the assembly of the pilus or for its specific functions. One class of minor pilins is required to initiate the formation of pili, and may form a complex similar to that identified in the related type II secretion system. Other, species-specific minor pilins in the type IV pilus system have been shown to promote additional functions such as DNA binding, aggregation and adherence. Here, we will review the structure and the function of the minor pilins from type IV pili.
Differences and overlaps between Phd studies in diagnostic microbiology in industrial and academic settings
Authors: van Belkum Alex , Pelegrin Andreu Coello , Datar Rucha , Goyal Manisha , Palmieri Mattia , Mirande Caroline , Chalansonnet Valérie , Orenga Sylvain 
Published: November 2019

AbstractIndustrial and academic needs for innovation and fundamental research are essential and not widely different. Depending on the industrial setting, research and development (R&D) activities may be more focused on the developmental aspects given the need to ultimately sell useful products. However, one of the biggest differences between academic and industrial R&D will usually be the funding model applied and the priority setting between innovative research and product development. Generalizing, companies usually opt for development using customer- and consumer-derived funds whereas university research is driven by open innovation, mostly funded by taxpayer’s money. Obviously, both approaches require scientific rigor and quality, dedication and perseverance and obtaining a PhD degree can be achieved in both settings. The formal differences between the two settings need to be realized and students should make an educated choice prior to the start of PhD-level research activities. Intrinsic differences in scientific approaches between the two categories of employers are not often discussed in great detail. We will here document our experience in this field and provide insights into the need for purely fundamental research, industrial R&D and current mixed models at the level of European funding of research. The field of diagnostics in clinical bacteriology and infectious diseases will serve as a source of reference.
Not Wanted: On Scharp’s Solution to the Liar
Authors: Pinder Mark 
Published: November 2019

AbstractKevin Scharp argues that the concept of truth is defective, and is therefore unable to play its intended role in natural language truth-conditional semantics. As such, for this theoretical purpose, Scharp constructs two replacements: ascending truth and descending truth. Scharp applies the resultant theory, AD semantics, to the liar sentence, thereby obtaining a novel solution to the liar paradox. The aim of the present paper is fourfold. First, I show that, contrary to Scharp’s claims, AD semantics in fact yields an inconsistency when applied to standard liar sentences. Second, I diagnose the problem: AD semantics mishandles negation. I propose an alternative treatment, resulting in what I call AD* semantics. Third, I show that AD* semantics gives Scharp the resources required to respond to an alleged revenge paradox that has been raised against his view. Finally, I argue that, these consequences notwithstanding, it remains unclear whether AD* semantics provides an adequate account of alethic paradoxes more generally.
Clinal variation in growth cessation and FTL2 expression in Siberian spruce
Authors: Li Lili , Chen Jun , Lascoux Martin 
Published: November 2019

AbstractForest trees exhibit strong patterns of local adaptation in phenological traits along latitudinal gradients. Previous studies in spruce have shown that variation at genes from the photoperiodic pathway and the circadian clock are associated to these clines but it has been difficult to find solid evidence of selection for some of these genes. Here, we used growth cessation, gene expression, and single nucleotide polymorphism (SNP) data at two major candidate loci,FLOWERING LOCUS T/TERMINAL FLOWER1-Like2(FTL2) andGIGANTEA(GI), as well as at background loci from a latitudinal gradient in Siberian spruce (Picea obovata) populations along the Ob River to test for clinal variation in growth cessation and at the two candidate genes. As in previous studies, there was a strong latitudinal cline in growth cessation that was accompanied by a significant cline in the expression ofFTL2. Expression ofFTL2was significantly associated with allele frequencies at some of theGI’s SNPs. However, the cline in allele frequency at candidate genes was not as steep as in a Norway spruce cline and in a parallel Siberian spruce cline studied previously and nonsignificant when a correction for population structure was applied. A McDonald-Kreitman test did not detect decisive evidence of selection onGI(pvalue = 0.07) and could not be applied toFTL2because of limited polymorphism. Nonetheless, polymorphisms contributed more to the increased neutrality index of PoGIthan to that of control loci. Finally, comparing the results of two previously published studies to our new dataset led to the identification of strong candidate SNPs for local adaptation inFTL2promoter andGI.
Genetic characterization of fragmented populations of Cinchona officinalis L. (Rubiaceae), a threatened tree of the northern Andean cloud forests
Authors: Cueva-Agila Augusta , Vélez-Mora Diego , Arias Daniela , Curto Manuel , Meimberg Harald , Brinegar Chris 
Published: November 2019

AbstractCinchona officinalisL., a tree species endemic to the cloud forests of the northern Tropical Andes, has suffered from historical bark harvesting for extraction of antimalarial compounds and has also experienced recent demographic losses from high rates of deforestation. Most remnant populations are found in severely degraded habitat on the edges of pastures while a minority are protected in private reserves. The goals of our research were to assess the genetic diversities of fragmented populations ofC. officinalisin the Loja province of southern Ecuador, characterize their phylogeographic distribution with respect to the region’s complex topography, and identify priority populations for conservation. Five nuclear microsatellite loci and the chloroplastrps16intron were used to analyze six populations. Moderate levels of genetic diversity were found in all populations although the more remote southern population (Angashcola) had slightly higher heterozygosity and allelic richness. There were no indications of recent genetic bottlenecks although anrps16intron haplotype was fixed in four populations. Genetic distance analysis based on microsatellite data placed the four easternmost populations in the same clade while the Angashcola population was the most divergent. Also, the most frequentrps16intron haplotype in Angashcola was not found in any other population. Although each of the studied populations should be protected from further deforestation and agricultural expansion, the Angashcola population deserves highest conservation priority.
Distribution and controls of petroliferous plays in subtle traps within a Paleogene lacustrine sequence stratigraphic framework, Dongying Depression, Bohai Bay Basin, Eastern China
Authors: Zhu Ji-Chang , Zou Cai-Neng , Feng You-Liang , Jiang Shu , Wu Wei-An , Zhu Ri-xiang , Yuan Miao 
Published: November 2019

AbstractThe characteristics of petroliferous plays in subtle traps within a sequence stratigraphic framework in the Dongying Depression are investigated in this study. Sand bodies within lowstand systems tracts (LSTs) of sequences, comprising incised-channel fills, sublacustrine fans, deltas in LSTs, controlled by syndepositional normal faults, and sand bodies within transgressive systems tracts (TSTs) to early highstand systems tracts (HSTs), consisting of beach bars, and turbidites, controlled by the prodelta slope, paleorelief, and syndepositional normal faults, are good subtle reservoirs. Mudstones and shale of deep lake subfacies in TSTs to early HSTs of sequences are source and cap rocks. Abnormal overpressure is the dominant dynamic factor for hydrocarbon migration from source rock to the subtle traps. Normal faults, sand bodies, and unconformities function as conduit systems. Sand bodies distributed in the abnormal overpressure source rocks within LSTs to early HSTs are petroliferous plays in lithologic traps. The petroliferous plays in stratigraphic traps are controlled by unconformities at margins of the Depression.
Modified method for fracability evaluation of tight sandstones based on interval transit time
Authors: Li Jing , Li Xiao-Rong , Zhan Hong-Bin , Song Ming-Shui , Liu Chen , Kong Xiang-Chao , Sun Lu-Ning 
Published: November 2019

AbstractFracability is a property that indicates how easy reservoir rocks can be fractured in hydraulic fracturing operations. It is a key parameter for fracturing design and evaluation. In order to utilize continuous logging data to predict fracability, synchronous tests of dynamic and static mechanical parameters of rocks under different confining pressures were conducted on 13 tight sandstone samples derived from the central Junggar Basin, China. A modified formula between dynamic and static mechanical parameters was established. Fracability of the tight reservoir in the Junggar Basin was then evaluated based on brittleness index, fracture toughness, and fracability index. The effectiveness of fracturing was analyzed combined with the oil testing curve after hydraulic fracturing. The results show that: (1) The distribution of oil-bearing formations in the studied area coincides well with stratum of higher fracability index. (2) The critical fracability index is determined to be 0.3, three formations are selected as fracturing candidates, and a thin mudstone interbed is identified in the oil-bearing formation. (3) Well testing curve verifies the reliability of the fracability evaluation method and the accuracy of the modified formula between dynamic and static mechanical parameters. This study provides useful information for improving fracturing operations of tight oil and gas reservoirs.
Vital bleaching for children with dental anomalies: EAPD members’ survey
Authors: Monteiro J. , Ashley P. F. , Parekh S. 
Published: November 2019

Abstract
Aim
Understand EAPD members’ practices of vital bleaching for children with dental anomalies.

Methods
An anonymous online survey sent via EAPD in January 2019, consisting of 13 questions with possible multiple answers and free text.

Results
110 responses from 24 countries were obtained. The majority worked in hospitals/universities (n = 69, 63%) or private practices (n = 50, 46%) and were specialists (n = 62, 57%) or senior academics (n = 35, 32%). Most respondents (n = 74 68%) did not provide vital bleaching for children. 88 respondents (80%) belonged to EU: of these, 46 (52%) were not aware of bleaching regulations. For respondents who provided bleaching 26 (72%) undertook home bleaching, using 10% carbamide peroxide (n = 21, 58%), most commonly for 2 weeks (n = 14, 39%), following establishment of the permanent dentition (n = 21, 58%). Deciding factors included: extent (n = 27, 75%) and shade (n = 26, 72%) of discolouration and child being teased by peers (n = 23, 64%). Main reasons for not bleaching included: concerns with side effects (n = 41; 55%) and not agreeing with bleaching (n = 23, 31%). Dentists who did not bleach managed a range of conditions, most frequently molar-incisor hypomineralisation (n = 57; 77%). The majority provided composite restorations with removal of tooth structure (n = 50; 68%) with a number opting for no treatment (n = 27, 37%).

Conclusion
This study shows wide variations in treatment of children’s dental anomalies across Europe. Fears of adverse effects and personal beliefs seemed to be the main deterrents to bleaching in children. Clinicians who provided bleaching tended to opt for more conservative techniques and to take children’s concerns into consideration.
The Financing of Small and Medium-Sized Enterprises: An Analysis of the Financing Gap in Brazil
Authors: Godke Veiga Marcelo , McCahery Joseph A. 
Published: November 2019

AbstractWhile small and medium-sized enterprises (SMEs) are important for economic growth and employment, they face numerous obstacles in accessing external finance. In this article, we review recent developments in the availability of financing for SMEs in Brazil, focusing on the greater use of equity and debt for SMEs. In assessing the barriers to external financing, we focus on the role of bank characteristics, market structure and variations of interest rate spreads across banks and time. Moreover, as banks retreat from SME financing, we examine the potential for SMEs to seek new sources of financing from private equity and venture capital funds. We examine the changes in the availability of bank loans between 2014 and 2016. By considering demand, we estimate the SME loan gap based on Central Bank and publicly available data. Our results show that the loan gap in Brazil is substantial.
Modelling urban networks using Variational Autoencoders
Authors: Kempinska Kira , Murcio Roberto 
Published: November 2019

AbstractA long-standing question for urban and regional planners pertains to the ability to describe urban patterns quantitatively. Cities’ transport infrastructure, particularly street networks, provides an invaluable source of information about the urban patterns generated by peoples’ movements and their interactions. With the increasing availability of street network datasets and the advancements in deep learning methods, we are presented with an unprecedented opportunity to push the frontiers of urban modelling towards more data-driven and accurate models of urban forms.In this study, we present our initial work on applying deep generative models to urban street network data to create spatially explicit urban models. We based our work on Variational Autoencoders (VAEs) which are deep generative models that have recently gained their popularity due to the ability to generate realistic images. Initial results show that VAEs are capable of capturing key high-level urban network metrics using low-dimensional vectors and generating new urban forms of complexity matching the cities captured in the street network data.
Testing Web Mapping and Active Learning to Approach Lidar Data
Authors: Forest Marion , Costa Laurent , Combey Andy , Dorison Antoine , Pereira Grégory 
Published: November 2019

ABSTRACTAfter acquiring 91 km2of lidar data from the Zacapu region, West Mexico, we confronted a series of issues that most archaeologists using this technology face. These include the large volume of data available, the limited training of potential “analysts,” the difficult development of a collective mapping tool and protocol, and the reliability of desk-based interpretation of archaeological features. In this article, we present an initiative conducted in 2015 and 2017 as an attempt to answer these methodological and pedagogical issues. We developed a web mapping platform to collectively interpret archaeological features using lidar-derived imagery and to train volunteer students to participate in this desk-based web mapping within a crowdsourcing framework. After evaluating the results of this initiative, we discuss the potential and limitations of this method for both lidar-based research and future training.
Conference Report: Global Health Security Alliance (GLoHSA), a Product of the World Health Summit
Authors: Quinn John M. , Haggenmiller Christian , Wilson James M. , McNamara Tracey , Goebbels Stefan , Hansen Jan-Cedric , Opitz Anja , Bourdeaux Margaret , Sullivan Richard 
Published: November 2019

ABSTRACTOver the past decade, the World Health Summit (WHS) has provided a global platform for policy-makers and decision-makers to interact with academics and practitioners on global health. Recently the WHS adopted health security into their agenda for transnational disease risks (eg, Ebola and antimicrobial resistance) that increasingly threaten multiple sectors. Global health engagement (GHE) focuses efforts across interdisciplinary and interorganizational lines to identify critical threats and provide rapid deployment of key resources at the right time for addressing health security risks. As a product of subject matter experts convening at the WHS, a special side-group has organically risen with leadership and coordination from the German Institute for Defense and Strategic Studies in support of GHE activities across governmental, academic, and industry partners. Through novel approaches and targeted methodology that maximize outcomes and streamline global health operational process, the Global Health Security Alliance (GloHSA) was born. This short conference report describes in more detail the GloHSA.
Fluidity, Phenotype and Afro-Latin Group Consciousness
Authors: Johnson Marcus 
Published: November 2019

AbstractRacial fluidity and mixed phenotype have been posited as critical barriers to politicized black identity in the region. Using an original, survey experiment in Panama, this paper finds that racial fluidity and phenotype significantly affect who identifies as black, but have relatively little impact on the strength of measures of black group consciousness. Rather than reducing the strength of group consciousness all together, racial fluidity and phenotype influence the salience of different measures of group consciousness. Afro-Panamanians with phenotypic features that stably predict black self-identification express stronger beliefs that racial discrimination is a problem in Panama (perceived discrimination) and greater dissatisfaction with the social standing of their in-group (polar power). In contrast, Afro-Panamanians with mixed phenotypic features express stronger in-group affect (pride), stronger belief in the efficacy of black collective mobilization (collective efficacy), and stronger linked fate.
Dietary protein insufficiency: an important consideration in fatty liver disease?
Authors: Ampong Isaac , Watkins Adam , Gutierrez-Merino Jorge , Ikwuobe John , Griffiths Helen R. 
Published: November 2019

AbstractDietary protein insufficiency has been linked to excessive TAG storage and non-alcoholic fatty liver disease (NAFLD) in developing countries. Hepatic TAG accumulation following a low-protein diet may be due to altered peroxisomal, mitochondrial and gut microbiota function. Hepatic peroxisomes and mitochondria normally mediate metabolism of nutrients to provide energy and substrates for lipogenesis. Peroxisome biogenesis and activities can be modulated by odd-chain fatty acids (OCFA) and SCFA that are derived from gut bacteria, for example, propionate and butyrate. Also produced during amino acid metabolism by peroxisomes and mitochondria, propionate and butyrate concentrations correlate inversely with risk of obesity, insulin resistance and NAFLD. In this horizon-scanning review, we have compiled available evidence on the effects of protein malnutrition on OCFA production, arising from loss in mitochondrial, peroxisomal and gut microbiota function, and its association with lipid accumulation in the liver. The methyl donor amino acid composition of dietary protein is an important contributor to liver function and lipid storage; the presence and abundance of dietary branched-chain amino acids can modulate the composition and metabolic activity of the gut microbiome and, on the other hand, can affect protective OCFA and SCFA production in the liver. In preclinical animal models fed with low-protein diets, specific amino acid supplementation can ameliorate fatty liver disease. The association between low dietary protein intake and fatty liver disease is underexplored and merits further investigation, particularly in vulnerable groups with dietary protein restriction in developing countries.
RETHINKING THE ETHIOPIAN RED TERROR: APPROACHES TO POLITICAL VIOLENCE IN REVOLUTIONARY ETHIOPIA
Authors: WIEBEL JACOB , ADMASIE SAMUEL ANDREAS 
Published: November 2019

ABSTRACTThis article examines the role of trade unions and of theKebele– the most local urban administrative structures of the Ethiopian state – in the making of the Red Terror, a period of unprecedented political violence that closely followed the Ethiopian revolution of 1974. Drawing on a broad range of new source materials – from labour union files to oral histories and East German State Security archives – this article shows how the Red Terror was in large part the product of synergies between diverse groups and actors within these structures, and how it was rooted in histories, motives, and collaborations that have scarcely been considered in the historiography of revolutionary Ethiopia. In turn, the Red Terror radically reshaped both trade unions andKebeleadministrations, affording Ethiopian state actors an unprecedented means of control over civil society and urban residents.
REEDUCATION CAMPS, AUSTERITY, AND THE CARCERAL REGIME IN SOCIALIST MOZAMBIQUE (1974–79)
Authors: MACHAVA BENEDITO 
Published: November 2019

AbstractThroughout the socialist experiment between 1974 and 1992, the Mozambique Liberation Front (Frelimo) ran a network of internment camps officially known as reeducation centers. Established in remote rural sites to mentally decolonize wayward members of urban society and putative enemies of the socialist revolution, the camps became a dumping ground for unwanted citizens accused of all kinds of wrongdoing. Although the Frelimo leaders envisioned a pedagogical institution that would undo the damage of colonialism by transforming reeducatees into new social beings, the gap between the idea of rehabilitation and the reality of detention was abysmally wide. Austerity – the order of the day throughout the fifteen years of socialist experiment in Mozambique – conditioned and defined the organic functioning of reeducation camps. Unlike internment camps elsewhere, Mozambique's camps were not strictly regimented. The carceral regime that emerged not only set Mozambique's reeducation centers apart from camps elsewhere, they were also far from the technocratic moralism and panoptic ambitions of the ruling party.