bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resveratrol reverses myogenic induction supression caused by high glucose through activating SIRT1/AKT/FOXO1 pathway
Authors: Author) Meiling Liu(Former Corresponding , Cheng Luyang , Li Xianglu , Ding Haifeng , Wang Hongzhi , Wang Manfeng , Author) Lu Gan(New Corresponding 
Published: February 2020

Abstract
Background: Long-term high glucose environment can cause muscle tissue atrophy, and then lead to musculoskeletal depression or even disability. Regenerative medicine is an extremely attractive select to solve this problem. Resveratrol is a compound which has various clinical therapeutic effects including regulating the myogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). So, the objective of this study is to observe if resveratrol affect myogenic induction of rat BMSCs under high glucose environment and explore the possible mechanism. Methods: Rat BMSCs were isolated and cultured. The phenotypes were identified when cultured to the third passage cells (P3 cells). Then the P3 cells were used to induce to differentiate into myogenic cells by using the conditioned medium. After grouping, glucose, resveratrol and EX527 (inhibitor of SIRT1) were added. The cell viability was measured by MTT assay. The myogenesis related protein was detected by immunofluorescence. The level of reactive oxygen species (ROS) and superoxide dismutase (SOD) activity were detected by use of assay kits. The cell cycle was assayed with flow cytometry. The expression of FOXO1, AKT, p-AKT, MyoD1 and Myogenin were measured by WB. All above indicators in different groups were quantified and compared. Results: During myogenic induction, after 72h treatment, high glucose (35 mmol/L) reduced cell viability and proliferation of rat BMSCs significantly, increased intracellular ROS levels clearly, decreased SOD activity obviously, and restrained AKT/FOXO1 pathway apparently. Resveratrol (15μmol/L) could regulate the process positively and reverse the suppression caused by high glucose partly through restoring cell proliferation and viability, reducing peroxidative damage and activating AKT/FOXO1 pathway. After pretreated the cells with EX527 (20 μmol/L), this reverse effect of resveratrol was eliminated. Conclusion: Resveratrol not only promoted myogenic induction of rat BMSCs, but also partially reversed myogenic induction supression of rat BMSCs caused by high glucose through activating SIRT1/AKT/FOXO1 pathway.
[Key words] resveratrol, bone marrow mesenchymal stem cells, myogenic induction, glucose, SIRT1, AKT, FOXO1
The research of Secretogranin III in periperal blood and vitreous of diabetic retinopathy
Authors: jiao mingfei , Sun Shuo , Wang Manqiao , Dong Lijie , Hu Bojie , Liu Juping , Li Wei , Li Xiaorong 
Published: February 2020

Abstract
Background: Secretory granulin III (SCG3) is a member of the secretory granule protein family that regulates the production of secretory granules. SCG3 has a role in reducing retinal vascular leakage and neovascularization in an animal model of diabetic retinopathy. This study aimed to study SCG3 in periperal blood and vitreous of human. Methods ： (1) Collecting diabetic retinopathy(DR) patients required vitrectomy, and patients requiring vitrectomy for other non-diabetic factors, retaining some of the vitreous, ELISA was used to detect SCG3 in vitreous. The grouping was divided according to the patients’ blood lipids, BMI (Body Mass Index, BMI) and analyzed. (2) Collecting peripheral blood of DR and non-diabetic patients, ELISAwas performed. Results: (1) A total of 43 cases with DR were collected, and 34 cases non-diabetic patients were collected. SCG3 in DR patients was higher than that of non-diabetic. After refinement grouping, it was found that SCG3 with DR and hyperlipidemia was higher than that of non-diabetic patients without hyperlipidemia. SCG3 with DR and hyperlipidemia was higher than that of DR patients without hyperlipidemia. SCG3 of patients with DR and high BMI was higher than that of non-diabetic with normal BMI. SCG3 with DR and high BMI was higher than that of non-diabetic patients with normal BMI. (2) SCG3 in plasma was minimal or could not be detected. Conclusion： In DR patients, up-regulation of vitreous SCG3 may be closely related to the pathogenesis of DR. However, SCG3 is almost difficult to detect in normal vessel vasculature, which may highlight its advantages in using anti-SCG3 drugs in babies and children in future.
Specific perception of the problem of tuberculosis by physicians from primary medical care
Authors: Chelnokova O. G. , Golovanova M. N. , Senin I. G. 
Published: February 2020

The objective: to study the perception of the problem of tuberculosis in physicians working in primary medical care and enhance motivation to implement tuberculosis control activities.Subjects and methods. A questionnaire survey was conducted in 114 physicians from primary medical care before and after the lecture on phthisiology. A subjective assessment of the knowledge on tuberculosis (self-assessment) and results of an objective assessment were studied and compared.Results. 30% of physicians had the fear of getting infected and developing tuberculosis. The level of theoretical phthisiologic knowledge among physicians from primary medical care is insufficient; the self-assessment of this knowledge is overstated in 58.8%.
The nature of Zn-phyllosilicates in the nonsulfide Mina Grande and Cristal zinc deposits (Bongará District, Northern Peru): The TEM-HRTEM and AEM perspective
Authors: Balassone Giuseppina , Scognamiglio Valentina , Nieto Fernando , Mondillo Nicola , Boni Maria , Cappelletti Piergiulio , Arfè Giuseppe 
Published: February 2020

AbstractZn-phyllosilicates are common minerals in nonsulfide Zn deposits and can give crucial information about the genesis of these oxidized mineralizations. They seldom represent the prevailing economic species but might have a significant impact on mineral processing. This study has been carried out on the Mina Grande and Cristal Zn-sulfide/nonsulfide deposits, which occur in the Bongará district (Amazonas region, northern Peru). The Cristal and Mina Grande orebodies are hosted by the sedimentary (prevailingly carbonate) successions of the Pucará Group (Condorsinga formation, Lower Jurassic), in an area affected by Neogene tectonics and characterized by Late Miocene and Pliocene-Early Pleistocene uplift phases (Andean and Quechua tectonic pulses). The Cristal deposit consists of both sulfide (sphalerite with minor pyrite and galena) and nonsulfide concentrations. The nonsulfides consists of smithsonite, hemimorphite, hydrozincite, chalcophanite, goethite, and greenockite, locally associated with Zn-bearing phyllosilicates. The Mina Grande deposit consists almost exclusively of Zn-oxidized minerals in limestone host rocks. The nonsulfides association consists of hydrozincite, hemimorphite, smithsonite, fraipontite, and Fe-(hydr)oxides, also containing a clayey fraction. The study deals with TEM-HRTEM and AEM investigations on clayey materials, to determine their crystal-chemical features and the origin of the complex Zn-clays-bearing parageneses. In both deposits, Zn-bearing illites (1Md and 2M polytypes) and I/S clay minerals (I3) are the main detected phases, with few compositions close to (Zn-bearing) muscovite. In the clayey fraction at Mina Grande, fraipontite, a Zn-bearing mica called K-deficient hendricksite, and (Zn-bearing) kaolinite also occur. Zn-illites and smectites (always containing Zn in variable amounts) characterize the mineral association at Cristal. The investigated compositional gap between di- and tri-octahedral Zn-phyllosilicates gives indications on the genetic relationships between them and advances on the knowledge of these species. The present work gives an insight into the Zn-bearing phyllosilicates systems by determining the amount/mode of metal incorporation in their lattices and understanding the relationships of natural occurring clay-rich complex associations, which can act as models for possible synthetic counterparts.
Radiation-induced defects in montebrasite: An electron paramagnetic resonance study of O – hole and Ti3+ electron centers
Authors: Toledo José R. , de Oliveira Raphaela , Dias Lorena N. , Chaves Mário L.C. , Karfunkel Joachim , Scholz Ricardo , Pinheiro Maurício V.B. , Krambrock Klaus 
Published: February 2020

Abstract
Montebrasite is a lithium aluminum phosphate mineral with the chemical formula LiAlPO4(Fx,OH1–x) and considered a rare gemstone material when exhibiting good crystallinity. In general, montebrasite is colorless, sometimes pale yellow or pale blue. Many minerals that do not have colors contain hydroxyl ions in their crystal structures and can develop color centers after ionization or particle irradiation, examples of which are topaz, quartz, and tourmaline. The color centers in these minerals are often related to O− hole centers, where the color is produced by bound small polarons inducing absorption bands in the near UV to the visible spectral range. In this work, colorless montebrasite specimens from Minas Gerais state, Brazil, were investigated by electron paramagnetic resonance (EPR) for radiation-induced defects and color centers. Although γ irradiation (up to a total dose of 1 MGy) did not visibly modify color, a 10 MeV electron irradiation (80 MGy) induced a pale greenish-blue color. Using EPR, O− hole centers were identified in both γ- or electron-irradiated montebrasite samples showing superhyperfine interactions with two nearly equivalent 27Al nuclei. In addition, two different Ti3+ electron centers were also observed. From the γ irradiation dose dependency and thermal stability experiments, it is concluded that production of O− hole centers is limited by simultaneous creation of Ti3+ electron centers located between two equivalent hydroxyl groups. In contrast, the concentration of O− hole centers can be strongly increased by high-dose electron irradiation independent of the type of Ti3+ electron centers. From detailed analysis of the EPR angular rotation patterns, microscopic models for the O− hole and Ti3+ electron centers are presented, as well as their role in the formation of color centers discussed and compared to other minerals.
Pemberdayaan Masjid sebagai Fungsi Sosial dan Ekonomi bagi Jamaah Pemegang Saham Unit Usaha Bersama
Authors: Suryorini Ariana 
Published: February 2020

<p>The function of the mosque is very universal, other than in the religious field, the mosque also has other functions which include religious/worship functions, educational functions, unifying functions of the people, social functions and economic functions. Islam places the mosque in a strategic position. Considering the strategic function of the mosque, it is necessary to provide good guidance, in terms of the physical building and the aspects of its prosperity. The mosque is expected to be a center of social and economic activity for its worshipers, not just a center for worship activities. Mosque empowerment is very important because it can provide a positive perspective related to the utilization of human resources through the empowerment of mosques for the welfare of Muslims. In the economic function of the empowerment of the Sirathal Mustaqim mosque through the activities of the Joint Business Unit (UB), whose ownership is pilgrims who have equity participation or capital ownership, which they refer to as shares of the business units they run, where profits are distributed to the owners or accompanying members of the capital in a meeting of capital owner members every year. The Joint Business Unit (UB) is named UB Rejeki Barokah, whose economic function activities are very diverse, both in the retail business of kiosks, services and goods credit business.</p><p> </p><p>Fungsi masjid sangat universal, selain di bidang keagamaan, masjid juga mempunyai fungsi lain yang meliputi fungsi keagamaan/peribadatan, fungsi pendidikan, fungsi pemersatu umat, fungsi sosial dan fungsi ekonomi. Islam menempatkan masjid dalam posisi yang strategis. Mengingat fungsi masjid yang strategis maka perlu pembinaan secara baik, dari segi fisik bangunan maupun segi kegiatan pemakmurannnya. Masjid diharapkan bisa menjadi pusat aktivitas sosial dan ekonomi bagi para jamaahnya, tidak hanya menjadi pusat kegiatan ibadah saja. Pemberdayaan masjid menjadi hal yang sangat penting karena dapat memberikan perspektif positif terkait pemanfaatan sumber daya manusia melalui pemberdayaan masjid untuk kesejahteraan umat Islam. Dalam fungsi ekonomi pemberdayaan masjid Sirathal Mustaqim melalui aktivitas unit Usaha Bersama (UB), yang kepemilikannya adalah jamaah yang mempunyai penyertaan atau kepemilikan modal, yang mereka sebut seperti saham dari unit usaha yang mereka jalankan, dimana keuntungannya dibagikan kepada jamaah pemilik atau penyerta modal tersebut dalam rapat anggota Pemilik modal setiap tahunnya. Unit Usaha Bersama (UB) tersebut bernama UB Rejeki Barokah, yang aktivitas fungsi ekononominya sangat beragam, baik dalam usaha retail kios, jasa maupun usaha perkreditan barang.</p>
Treatment of high-concentration chromium-containing wastewater by sulfate-reducing bacteria acclimated with ethanol
Authors: Li Xilin , Fan Ming , Liu Ling , Chang Jinghua , Zhang Jiawen 
Published: February 2020

Abstract
In order to solve the problem of difficult treatment of high-concentration chromium-containing wastewater, sulfate-reducing bacteria (SRB) with a high tolerance of hexavalent chromium and a strong ability to reduce the compound were isolated from sludge from a sedimentation tank in a leather industrial park and was identified as Desulfovibrio by morphological observation, routine physiological and biochemical determination, 16S rDNA sequencing and phylogenetic tree construction. After ethanol acclimation, a strain of SRB that could reduce chromium (CR-1) was selected as the research object. The optimum growth conditions for hexavalent chromium removal by the strain were determined by single-factor analysis. The chromium removal mechanism of the strain was analyzed, and a kinetic model of the reduction process was established. The chromium-reducing ability of the strain was 500 mg/L, the optimum pH value was 7, the optimum temperature was 35 °C, the optimum cultivation time was 24 h, and the optimum ratio of bacteria to waste (volume ratio of bacterial solution dosage and chromium-containing wastewater) was 1:5. The mechanism of treatment of Cr(VI) by this strain is mainly based on the reduction of Cr(VI) by H2S accumulated in the cultured bacterial solution and the small amount of H2S generated by bacterial reductase, bacterial growth and SO42− reduction in the waste liquid.
Pine Bark Phenolic Extracts, Current Uses, and Potential Food Applications: A Review
Authors: Dridi Wafa , Bordenave Nicolas 
Published: February 2020

Purpose:
To summarize the main findings from research on food uses of Pine Bark Phenolic Extracts
(PBPE), their origin, methods of extraction, composition, health effects, and incorporation into food products.

Methods:
A narrative review of all the relevant papers known to the authors was conducted.

Results:
PBPE are mainly extracted from the bark Pinus pinaster. They are generally rich in procyanidins and
their effects on health in the form of nutritional supplements include effect on some forms of cancer, on diabetes,
on eye and skin health. Their method of extraction influences greatly their composition and yield, and commercially
suitable methods are still to be developed. Incorporation into food products raises challenges related to
bioavailability and subsequent bioactivity and sensory properties of the final products.

Conclusion:
PBPE represent an opportunity for the development of functional foods with phenolic-rich bioactive
compounds.
Plant Virus Nanoparticles for Vaccine Applications
Authors: Santoni Mattia , Zampieri Roberta , Avesani Linda 
Published: February 2020

:
In the rapidly evolving field of nanotechnology, plant virus nanoparticles (pVNPs) are
emerging as powerful tools in diverse applications ranging from biomedicine to materials science. The
proteinaceous structure of plant viruses allows the capsid structure to be modified by genetic engineering
and/or chemical conjugation with nanoscale precision. This means that pVNPs can be engineered
to display peptides and proteins on their external surface, including immunodominant peptides derived
from pathogens allowing pVNPs to be used for active immunization. In this context, pVNPs are safer
than VNPs derived from mammalian viruses because there is no risk of infection or reversion to pathogenicity.
Furthermore, pVNPs can be produced rapidly and inexpensively in natural host plants or
heterologous production platforms.

:
In this review, we discuss the use of pVNPs for the delivery of peptide antigens to the host immune in
pre-clinical studies with the final aim of promoting systemic immunity against the corresponding
pathogens. Furthermore, we described the versatility of plant viruses, with innate immunostimulatory
properties, in providing a huge natural resource of carriers that can be used to develop the next generation
of sustainable vaccines.
Amyloid β-induced Mesenteric Inflammation in an Alzheimer’s Disease Transgenic Mouse Model
Authors: Ano Yasuhisa , Ikado Kumiko , Uchida Kazuyuki , Nakayama Hiroyuki 
Published: February 2020

Background:
Alzheimer’s disease (AD) is a neurodegenerative disorder histopathologically
characterized by the accumulation of amyloid β (Aβ) peptides and inflammation associated with activated
microglia. These features are well investigated in the central nervous system using AD-model
mice; however, peripheral inflammation in these mice has not been investigated well.

Objective:
We evaluated the inflammatory responses, especially myeloid dendritic cells (mDCs), in peripheral
lymphoid tissues in AD-model mice to determine their association with Aβ deposition.

Methods:
We collected lymphocytes from mesenteric lymphoid nodes (MLNs) and Peyer’s patches
(PPs) of 5×FAD transgenic mice used as an AD model. Lymphocytes were analyzed using a flow cytometer
to characterize mDCs and T cells. Collected lymphocytes were treated with Aβ1-42 ex vivo to
evaluate the inflammatory response.

Results:
We observed elevated levels of inflammatory cytokines and chemokines including interleukin
(IL)-12 and macrophage inflammatory protein-1α in mDCs from MLNs and PPs and reduced levels of
programmed death-ligand-1, an immunosuppressive co-stimulatory molecule, on the surface of mDCs
from 5×FAD mice. Additionally, we found increases in interferon (IFN)-γ-producing CD4- or CD8-
positive T cells in MLNs were increased in 5αFAD mice. Moreover, ex vivo treatment with Aβ peptides
increased the production of IL-12 and IFN-γ by lymphocytes from 5×FAD mice.

Conclusion:
The present study showed that pro-inflammatory mDC and T cells were induced in MLNs
and PPs of 5×FAD mice.
Synthesis and Biological Evaluation of New Naphthoquinones Derivatives
Authors: Ourhzif El-Mahdi , Decombat Caroline , Abrunhosa-Thomas Isabelle , Delort Laetitia , Khouili Mostafa , Akssira Mohamed , Caldefie-Chezet Florence , Chalard Pierre , Troin Yves 
Published: February 2020

:
New substituted 1,4-naphthoquinones have been prepared in good overall yields through the
naphthol route. The cytotoxicity of these compounds was tested in vitro on MCF-7 breast tumor cells. The
most active compound 14 displayed an IC50 of 15μM.

Objective:
To investigate the cytotoxicity of new naphthoquinones derivatives on MCF-7 cells.

Methods:
Synthesis of new naphtoquinones derivatives and in vitro evaluation of their cytotoxicity on MCF-7
cells (rezasurin cell-based assay).

Results:
Starting from Ethyl 4-hydroxy-6,7-dimethoxy-2-naphthoate, four naphthoquinones were prepared and
exhibited substantial cytotoxicity against MCF-7 cells.

Conclusion:
Preliminary studies of the structure-activity relationship have shown the influence of the structural
parameters and, in particular, the nature of the naphthoquinone side chain.
Phytochemical and Pharmacophoric Fragment Based Anticancer Drug Development
Authors: Rochlani S.P. , Dahiwade L.K. , Choudhari P.B. 
Published: February 2020

Background:
Cancer is the leading cause of death in the current decade. With the advancement
in scientific technologies various treatments had been introduced but they suffer from
numerous side effects. The root cause of cancer is alteration in the cell cycle which generates cancerous
cells. Development of new lead which specifically target cancerous cells is needed to reduce
the side effect and to overcome multidrug resistance.

Objective:
Design and development of anticancer leads targeting colchicine site of microtubules using
structurally screened phytofragments is the primary objective of this work.

Materials and Methods:
Bioactive fragments of phytoconstituents were identified from a large dataset
of phytochemicals. The identified phytofragments were used to design structures which were
screened for virtual interactions with colchicine site of microtubules. Selected set of designed molecules
was further screened for drug like properties and toxicity. The designed molecules which
surpassed virtual filters were synthesized, characterized and further screened for anticancer potential
against HEPG2 liver cancer cell line.

Results:
A novel series of chalcones was designed by phytofragment based drug design. Synthesized
compounds showed profound anticancer activity comparable to standard, 5-fluoro uracil. In
the present communication, rational development of anticancer leads targeting colchicine site of
microtubules has been done by integrating pocket modeling and virtual screening with synthesis
and biological screening.

Conclusion:
In this present work, we found that compounds S4 and S3 showed specific interaction
with colchicine site of microtubules and desirable anticancer activity. Further optimization of the
lead could yield drug like candidate with reduced side effects and may overcome multidrug resistance.
Toddalia asiatica L. - A Rich Source of Phytoconstituents with Potential Pharmacological Actions, an Appropriate Plant for Recent Global Arena
Authors: Alagaraj Praveena , Muthukrishnan Suriyavathana 
Published: February 2020

:
Toddalia asiatica L. is used for a long period against various chronic diseases. The main
objective of the present review is to search the literature for the folklore uses, phytochemicals isolated
and their pharmacological properties of Toddalia asiatica L. The individual plant parts possess
many phytoconstituents with therapeutic actions. The root bark of the plant is antimalarial, antipyretic
and carminative. Plant leaves and stems have bitter taste and analgesic in nature. Leaf essential oil
is used in relieving rheumatic arthritis, sprains, contusions, intercostal neuralgia, cough, malaria,
dysentery and gastralgia. This compiled data may be helpful for the researchers to focus on the areas
of research yet to be discovered. Complete information about the plant has been collected from various
books, journals etc.