bluuebunny/crossref_metadata_2025_split huggingface dataset:
Subarticular Osteophytes in Patients with Osteoarthritis of the Hip. A Histomorphometric Cross-sectional Case-control Study.
Authors: Klose-Jensen Rasmus , Nielsen Andreas Wiggers , Hartlev Louise Brøndt , Thomasen Jesper Skovhus , Boel Lene Warner Thorup , Laursen Mogens , Keller Kresten Krarup , Hauge Ellen-Margrethe 
Published: August 2020

Abstract
Objective

The objective of this cross-sectional case-control study was to determine the prevalence and size of marginal and subarticular osteophytes in patients with osteoarthritis (OA), and to compare these to that of a control group.

Design

We investigated femoral heads from 25 patients with OA following hip replacement surgery, and 25 femoral heads from a control group obtained post-mortem . The area and boundary length of the femoral head, marginal osteophytes, and subarticular osteophytes were determined with histomorphometry. Marginal osteophytes were defined histologically as bony projections at the peripheral margin of the femoral head, while subarticular osteophytes were defined as areas of bone that expanded from the normal curvature of the femoral head into the articular cartilage.

Results

The prevalence of OA patients with marginal- and subarticular osteophytes were 100% and 84%, respectively. Whereas the prevalence of the participants in the control group with marginal- and subarticular osteophytes were 56% and 28%, respectively.

The area and boundary length of marginal osteophytes was (median (Interquartile range)) 165.3mm 2 (121.4 – 254.0) mm 2 and 75.1 mm (50.8 – 99.3) mm for patients with OA compared to 0 mm 2 (0 – 0.5) mm 2 and 0 mm (0 – 0.5) mm for the control group (P < 0.001). For the subarticular osteophytes, the area and boundary length was 1.0 mm 2 (0 – 4.4) mm 2 and 1.4 mm (0 – 6.5) mm for patients with OA compared to 0 mm 2 (0 – 0.5) mm 2 and 0 mm (0 – 0.5) mm for the control group (P < 0.001).

Conclusion

As expected, both marginal- and subarticular osteophytes at the femoral head, were more frequent and larger in patients with OA than in the control group. However, in the control group, subarticular osteophytes were more prevalent than expected from the minor osteophytic changes at the femoral head margin, which may suggest that subarticular osteophytes are an early degenerative phenomenon that ultimately might develop into clinical osteoarthritis.
Genetically-regulated transcriptomics &amp; copy number variation of proctitis points to altered mitochondrial and DNA repair mechanisms in individuals of European ancestry
Authors: Pathak Gita A , Polimanti Renato , Silzer Talisa K , Wendt Frank R , Chakraborty Ranajit , Phillips Nicole R 
Published: August 2020

Abstract
BACKGROUND: Proctitis is an inflammation of the rectum and may be induced by radiation treatment for cancer. The genetic heritability of developing radiotoxicity and prior role of genetic variants as being associated with side-effects of radiotherapy necessitates further investigation for underlying molecular mechanisms. In this study, we investigated gene expression regulated by genetic variants, and copy number variation in prostate cancer survivors with radiotoxicity.METHODS: We investigated proctitis as a radiotoxic endpoint in prostate cancer patients who received radiotherapy (n=222). We analyzed the copy number variation and genetically regulated gene expression profiles of whole-blood and prostate tissue associated with proctitis. The SNP and copy number data were genotyped on Affymetrix® Genome-wide Human SNP Array 6.0. Following QC measures, the genotypes were used to obtain gene expression by leveraging GTEx, a reference dataset for gene expression association based on genotype and RNA-seq information for prostate (n= 132) and whole-blood tissue (n=369). RESULTS: In prostate tissue, 62 genes were significantly associated with proctitis, and 98 genes in whole-blood tissue. Six genes - CABLES2, ATP6AP1L, IFIT5, ATRIP, TELO2, and PARD6G were common to both tissues. The copy number analysis identified seven regions associated with proctitis, one of which (ALG1L2) was also associated with proctitis based on transcriptomic profiles in the whole-blood tissue. The genes identified via transcriptomics and copy number variation association were further investigated for enriched pathways and gene ontology. Some of the enriched processes were DNA repair, mitochondrial apoptosis regulation, cell-to-cell signaling interaction processes for renal and urological system, and organismal injury.CONCLUSIONS: We report gene expression changes based on genetic polymorphisms. Integrating gene-network information identified these genes to relate to canonical DNA repair genes and processes. This investigation highlights genes involved in DNA repair processes and mitochondrial malfunction possibly via inflammation. Therefore, it is suggested that larger studies will provide more power to infer the extent of underlying genetic contribution for an individual’s susceptibility to developing radiotoxicity.
Study on the community structure&nbsp;and function of symbiotic bacteria at different growth and developmental stages of Hypsizygus marmoreus
Authors: sun shujing , Li Fan , Xu Xin , Liu Yunchao , Kong Xuqiang , Chen Jianqiu , Liu Ting , Chen Liding 
Published: August 2020

Abstract
Background: The symbiotic bacteria associated with edible fungi are valuable microbial resources worthy of in-depth exploration. It is important to analyze the community structure and succession of symbiotic bacteria in mushrooms. This can also assist in the isolation of growth-promoting strains that have an essential relationship with the cultivation cycle as well as the agronomic traits and yields of fruiting bodies.Results: In all of the samples from cultivation bags of Hypsizygus marmoreus, 34 bacterial phyla were detected. Firmicutes was the most abundant bacterial phylum (78.85%). The genus Serratia showed an exponential increase in abundance in the cultivation bags in the mature period, reaching a peak abundance of 55.74% and becoming the dominant symbiotic flora. The most predominant strain was Serratia odorifera HZSO-1, and its abundance increased with the amount of hyphae of H. marmoreus. Serratia odorifera HZSO-1 could reside in the hyphae of H. marmoreus, promote growth and development, shorten the fruiting cycle by 3-4 days, and further increase the fruiting body yield by 12%. Conclusions: This study is a pioneering demonstration of the community structure of the symbiotic microbiota and bacteria-mushroom interaction in the growth and development of edible fungi. This work lays a theoretical foundation to improve the industrial production of mushrooms with symbiotic bacteria as assisting agents.
Associations of Socioeconomic Factors with Cause-specific Mortality and Burden of Cardiovascular Diseases: Findings from the Vital Registration in Urban Shanghai, China, during 1974-2015
Authors: Zhang Lijuan , Li Qi , Han Xue , Wang Shuo , Li Peng , Ding Yibo , Zhang Tao , Zhao Jia , Chen Yifan , Liu Jiluo , Li Jue , Tan Xiaojie , Liu Wenbin , Zhang Rong , Cao Guangwen 
Published: August 2020

Abstract
Background: Cardiovascular disease (CVD) is the leading cause of mortality worldwide. The effect of socioeconomic factors on cause-specific mortality and burden of CVD is rarely evaluated in low- and middle-income countries, especially in a rapidly changing society. Methods: Original data were derived from the vital registration system in Yangpu, a representative, population-stable district of urban Shanghai, China, during 1974-2015. Temporal trends for the mortality rates and burden of CVD during 1974-2015 were evaluated using Joinpoint Regression Software. The burden was evaluated using age-standardized person years of life loss per 100,000 persons (SPYLLs). Age-sex-specific CVD mortality rates were predicted by using age-period-cohort Poisson regression model. Results: A total of 101,822 CVD death occurred during 1974-2015, accounting for 36.95% of total death. Hemorrhagic stroke, ischemic heart disease, and ischemic stroke were the 3 leading causes of CVD death. The age-standardized CVD mortality decreased from 144.5/100,000 to 100.7/100,000 in the residents (average annual percentage change [AAPC] -1.0, 95% confidence interval [CI] -1.7 to -0.2), which was mainly contributed by women (AAPC -1.3, 95% CI -2.0 to -0.7), not by men. Hemorrhagic stroke, the major CVD death in the mid-aged population, decreased dramatically after 1991. The crude mortality of ischemic heart disease kept increasing but its age-adjusted mortality decreased continually after 1997. SPYLLs of CVD death increased from 1974 to 1986 (AAPC 2.1, 95% CI 0.4 to 3.8) and decreased after 1986 (AAPC 1.8, 95% CI -2.3 to -1.3). These changes were in concert with the implementation of policies including extended medical insurance coverage, pollution control, active prophylaxis of CVD including lifestyle promotion, and national health programs. The mortality of CVD increased in those born during 1937-1945, a period of the Japanese military occupation, and during 1958-1965, a period including the Chinese Famine. Sequelae of CVD and ischemic heart disease are predicted to be the leading causes of CVD death in 2029. Conclusions: Exposure to serious malnutrition in early life might increase CVD mortality in later life. Improvements in medical services, pollution control, and lifestyle could decrease CVD death. New strategy is needed to prevent the aging-related CVD death and burden in the future.
Polyporus polysaccharide regulates proliferation, apoptosis and migration of bladder cancer cells by polarizing macrophages to M1 subtype in tumor microenvironment
Authors: Jia Wenyu , Luo Siwan , Lai Gena , Li Shiqi , Huo Shuai , Li Meifang , Zeng Xing 
Published: August 2020

Abstract
Background: Polyporus polysaccharide (PPS), an active ingredient of traditional Chinese medicinal Polyporus umbellatus, has multiple biological functions, such as anti-cancer, immune-regulating and hepatoprotective activities. The purpose of this study was to investigate the mechanism of PPS activated macrophages in the treatment of bladder cancer.Methods: 100 ng/mL Phorbol myristate acetate (PMA) was used to induce THP-1 human leukemic cells as a macrophage model. Flow cytometry was used to detect the expression of CD14 and CD68 to verify the establishment of macrophage model. After that, Macrophages derived from THP-1 were treated with different concentrations of PPS (1,10 and 100 ug/mL). Flow cytometry and RT-PCR were used to detected the expression of CD16, CD23, CD86, CD40 and interleukin (IL)-Iβ, iNOS mRNA. ELISA was used to test the change of IL-1β and TNF-α in macrophage after the treatment with PPS. The conditioned medium from PPS-polarized macrophages was used to detect the effect of activated macrophages on bladder cancer. MTT assay, 5-ethynyl-2¢-deoxyuridine assay, flow cytometry, Transwell assay, and Western blot analysis were used to detect the effects of polarized macrophages on the viability, proliferation, apoptosis, and migration of bladder cancer cells. Western blot was also used to analysis the change of JAK2/NF-κB pathway protein.Results: PPS promoted the expression of pro-inflammatory factors, such as IL-Iβ, TNF-α and iNOS, and surface molecules CD86, CD16, CD23, and CD40 in macrophages and then polarized macrophages to M1 type. The results demonstrated that activated macrophages inhibited the proliferation of bladder cancer cells, regulated their apoptosis, and inhibited migration and epithelial–mesenchymal transformation (EMT). JAK2/NF-κB pathways were downregulated in the anti-bladder cancer process of activated macrophages. Conclusion: The findings indicated that PPS inhibited the proliferation and progression of bladder cancer by the polarization of macrophages to M1 type, and JAK2/NF-κB pathway was downregulated in the process of anti-bladder cancer.
Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product
Authors: Guo Rui , Guo Shuchen , Gao Xiong , Wang Huaiyou , Hu Weihui , Duan Ran , Dong Tina T. X. , Tsim Karl W. K. 
Published: August 2020

Abstract
Background: Danggui Buxue Tang (DBT), an ancient Chinese herbal decoction containing Astragali Radix and Angelicae Sinensis Radix at a ratio of 5: 1, is prescribed for menopausal women. Flavonoids and its flavonoid glycosides are considered as the major active ingredients within the herbal decoction; however, their amount is not controllable during the preparation. Besides, the aglycons within DBT are believed to have better gut absorption and pharmacological efficacy. Methods: The herbal extract of DBT was fermented with Lactobacillus plantarum. The amounts of flavonoid glucosides and its aglycones in the fermented product were analyzed by using UPLC-MS/MS. In addition, in vitro assays were employed to evaluate the efficacy of the fermented DBT in regulating the activities of α-glucosidase, α-amylase and lipase, as well as their antioxidant capacity (DPPH and T-OAC assays) and anti-glycation property (BSA-methylglyoxal, BSA-fructose, and arginine-methylglyoxal models).Results: The fermentation of DBT with L. plantarum drove a completed conversion of calycosin-7-O-β-D-glucoside and ononin to calycosin and formononetin, respectively. The chemical transformation could be probably mediated by β-glycosidase within the fermented product. Several in vitro assays corresponding to anti-diabetic functions were compared between parental DBT against its fermented product, which included the activities against α-glucosidase, α-amylase and lipase, as well as anti-oxidation and anti-glycation. The fermented DBT showed increased activities in inhibiting α-glycosidase, suppressing DPPH radical-scavenging and anti-glycation, as compared to the original herbal product. Conclusion: These results suggested that DBT being fermented with the probiotic L. plantarum could pave a new direction for fermentation of herbal extract, as to strengthen its pharmacological properties in providing health benefits.
Bigger doesn’t mean bolder: Behavioral variation of four wild rodent species to novelty and predation risk following a fast-slow continuum
Authors: Best Ian Nicholas , Shaner Pei-Jen Lee , Lo Hsuan-Yi , Pei Kurtis Jai-Chyi , Kuo Chi-Chien 
Published: August 2020

Abstract
Background: Understanding how wild species respond to novel situations with associated risk can provide valuable insights for inter-specific behavioral variation and associations with pace-of-life (POL). Rodents, a globally distributed and diverse taxonomic group, have been the subjects of countless studies emulating risky situations. Controlled laboratory experiments with a focus on wild-caught species provide the opportunity to test fine-scale behavioral responses to contexts of risk with ecological implications. For example, assessing the importance of predator cues eliciting antipredator responses, as well as whether wild rodents embody behavioral plasticity and repertoires, illustrated by habituation and variation in behavioral traits, respectively. Results: In this comparative study, we examined multiple behavioral responses of four rodent species in eastern Taiwan (three native species Mus caroli, Apodemus agrarius, Rattus losea, and one invasive, Rattus exulans) exposed to an unfamiliar microenvironment and novel cue from an allopatric predator, the leopard cat (Prionailurus bengalensis). All wild-caught animals were subjected to two consecutive nights of experimental trials in a laboratory setting. Behavioral responses to a novel situation during the first trial differed between species; smaller species investing more time in non-defensive behaviors compared to the larger species. More specifically, the smaller species M. caroli and A. agrarius allocated more time to exploration and foraging, whereas the larger rat species R. exulans and R. losea spent more time motionless or concealing. During the second trial, the addition of leopard cat cues did not elicit antipredator behaviors, but rather, rodents were found to exhibit increased non-defensive behaviors, specifically foraging efforts. Conclusions: Our results suggest that these four species do largely follow a behavioral fast-slow continuum with the two smaller mice species demonstrating increased boldness in a novel context compared to the larger rat species. Also, the wild populations of rodents in eastern Taiwan may be naïve to leopard cats. Finally, the rodents in our study demonstrated habituation to the microenvironment, indicating they possess adaptive capacity.
WRN modulates translation by influencing mRNA export from the nucleus through its interaction with the export receptor NXF1 in HeLa cancer cell
Authors: Iglesias-Pedraz Juan Manuel , Jara Diego Matia Fossatti , Felice Valeria Del Carmen Valle-Riestra , Visalaya Sergio Rafael Cruz , Felix Jose Antonio Ayala , Comai Lucio 
Published: August 2020

Abstract
Background The Werner syndrome protein (WRN) belongs to the RecQ family of helicases and its loss of function results in the premature aging disease Werner syndrome (WS). We previously demonstrated that an early cellular change induced by WRN depletion is a posttranscriptional decrease in the levels of enzymes involved in metabolic pathways that control macromolecular synthesis and protect from oxidative stress. This metabolic shift is tolerated by normal cells but causes mitochondria dysfunction and acute oxidative stress in rapidly growing cancer cells, thereby suppressing their proliferation.Results To identify the mechanism underlying this metabolic shift, we examined global protein synthesis and mRNA nucleocytoplasmic distribution after WRN knockdown. We determined that WRN depletion in HeLa cells attenuates global protein synthesis without affecting the level of key components of the mRNA export machinery. We further observed that WRN depletion affects the nuclear export of mRNAs and demonstrated that WRN directly interacts with mRNA and the mRNA export receptor Nuclear Export Factor 1 (NXF1).Conclusions Our findings suggest that WRN influences the export of mRNAs from the nucleus through its interaction with the NXF1 export receptor thereby affecting cellular proteostasis. In summary, we identified a new partner and a novel function of WRN, which is especially important for the proliferation of cancer cells.
The relationship between ratio of serum triglyceride to high-density lipoprotein cholesterol and frequency of sarcopenia in Chinese community adults
Authors: Wang Na , Chen Mengjun , Fang Danhong 
Published: August 2020

Abstract
Background In a previous study, the high ratio of serum triglyceride to high-density lipoprotein cholesterol (TG/HDL-C) was relevant to a high risk of sarcopenia in Korean old males. In this study, the purpose was to discover the correlation in Chinese community adults.Methods Chinese adults who had physical examinations from May 2016 to August 2017 at the First Affiliated Hospital of Wenzhou Medical University were involved in this study. The univariable and multivariable analyses were applied to evaluate possible effect and an association was found in a subgroup analysis.Results In total, 2613 adults were involved in the study, with 13.85% presenting sarcopenia. Individuals with TG/HDL-C ratios <2.78 or ≥2.78 were categorized as TG/HDL-Clow or TG/HDL-Chigh, respectively. 1266 individuals were in high group and the remaining were in the low group. By univariate and multivariable analyses, the ratio of TG/HDL-C remained independent association with sarcopenia status(OR: 0.63; 95%CI: 0.49-0.81). Besides, the effect of the TG/HDL-C ratio kept significantly favorable (OR<1.00) in most subgroups, except in groups aged＞65 years and in the overweight population. Conclusions The negative correlation between sarcopenia and TG/HDL-C ratio was discovered in Chinese community population, particularly in subjects aged <65 years and in non-overweight population.
Proposal of a new equation for estimating resting energy expenditure for acute kidney injury patients on dialysis. A Machine Learning Approach.
Authors: ponce daniela , de Goes Cassiana Regina , de Andrade Luis Gustavo Modelli 
Published: August 2020

Abstract
The objective of this study was, therefore, to develop a new predictive equation of resting energy expenditure (REE) for acute kidney injury patients (AKI) on dialysis. Material and methods: A cross-sectional descriptive study has been carried out in 114 AKI patients on dialysis and mechanical ventilation consecutively selected, aged between 19 and 95 years. For the construction of the predictive model, 80% of the cases were randomly separated to train and 20% of unused cases for validation. Several machine learning models were tested in the training data: linear regression with Stepwise, rpart, support vector machine with radial kernel, generalized boosting machine, and random forest. The models were selected by 10-fold cross-validation and the performances evaluated by the root mean square error (RMSE). Results: There were 364 indirect calorimetry (IC) measurements in 114 patients, mean age of 60.65 ± 16.9 years and 68.4% were males. The average REE was 2081 ± 645 kcal. REE was positively correlated with C-reactive protein, minute volume (MV), expiratory positive airway pressure, serum urea, body mass index and inversely with age. The principal variables included in the selected model were age, BMI, use of vasopressors, expiratory positive airway pressure, minute volume, C-reactive protein, temperature, and serum urea. The final r-value in the validation set was 0.69. Conclusion: We propose a new predictive equation for estimating the REE for AKI patients on dialysis that use a non-linear approach with better performance than actual models.
The Impact of COVID-19 Pandemic on Dental Practice in Iran: A questionnaire-based report
Authors: Ahmadi Hanie , Ebrahimi Alireza , Ghorbani Farhad 
Published: August 2020

Abstract
Background: The highly contagious nature of the novel coronavirus, besides the fact that dental procedures commonly generate blood and saliva droplets that are the routes of contagion lead to the closure of many dental clinics. In the present study, we aimed to evaluate the impact of coronavirus disease 2019 (COVID-19) pandemic on dental practice by conducting an online questionnaire among the Iranian dental practitioners and finding their perspectives on the subject.Methods: This report is based on a questionnaire that was conducted among Iranian dentists. The survey included questions that evaluate the dentists’ attitudes toward the COVID-19 pandemic and its effect on their personal life, financial status, and the quality of dental services for patients. Results: Overall, 240 dentists contributed to this study (214 general dentists and 26 specialists). The majority of the participants (n=170, 70%) did not perform non-emergency procedures during the pandemic. More than half of the participants (n=156, 64%) believed that the triage of patients should be done regarding the COVID-19 symptoms. Most of the dentists (n=210, 87%) had a problem with providing personal protective equipment during the pandemic. Moreover, 97% (n=234) of the participants reported that they encountered a decrease in their financial income since the eruption of the pandemicConclusion: Dental health care workers are at the highest risk of contracting COVID-19. Iranian dentists executed standard protocols more cautiously during the pandemic. Besides, they preferred to lower their work hours and limit dental procedures to emergency treatments until the end of the pandemic. It is recommended that public organizations should support the dental practitioners during the pandemic.