bluuebunny/crossref_metadata_2025_split huggingface dataset:
The systemic and governmental agendas in presidential attention to climate change in Mexico 1994–2018
Authors: Balderas Torres Arturo , Lazaro Vargas Priscila , Paavola Jouni 
Published: January 2020

AbstractAmbitious climate action requires sustained long-term attention from political leaders. To understand how climate change entered the political agenda in a developing country, we examine from an agenda-setting perspective the attention paid by Mexican presidents to this issue from 1994 to 2018. We perform a longitudinal analysis of 968 documents referring to climate change published by four presidencies to describe changes in attention levels over time and to determine how changes in international agreements and public policies (i.e. systemic agenda) and National Development Plans (NDPs)(i.e. governmental agenda) influence them. Our results indicate international agreements and national legislation establish a baseline for inclusion of climate change into governmental actions. Agenda changes driven by international agreements result in reactive changes in attention, while ambitious approaches are aligned with proactive NDPs. Our results also indicate public awareness and electoral periods can open windows of opportunity for reframing agendas and promoting ambitious climate action.
Genetic deletion of mast cell serotonin synthesis prevents the development of obesity and insulin resistance
Authors: Yabut Julian M. , Desjardins Eric M. , Chan Eric J. , Day Emily A. , Leroux Julie M. , Wang Bo , Crane Elizabeth D. , Wong Wesley , Morrison Katherine M. , Crane Justin D. , Khan Waliul I. , Steinberg Gregory R. 
Published: January 2020

AbstractObesity is linked with insulin resistance and is characterized by excessive accumulation of adipose tissue due to chronic energy imbalance. Increasing thermogenic brown and beige adipose tissue futile cycling may be an important strategy to increase energy expenditure in obesity, however, brown adipose tissue metabolic activity is lower with obesity. Herein, we report that the exposure of mice to thermoneutrality promotes the infiltration of white adipose tissue with mast cells that are highly enriched with tryptophan hydroxylase 1 (Tph1), the rate limiting enzyme regulating peripheral serotonin synthesis. Engraftment of mast cell-deficient mice with Tph1−/− mast cells or selective mast cell deletion of Tph1 enhances uncoupling protein 1 (Ucp1) expression in white adipose tissue and protects mice from developing obesity and insulin resistance. These data suggest that therapies aimed at inhibiting mast cell Tph1 may represent a therapeutic approach for the treatment of obesity and type 2 diabetes.
Chromosome arm aneuploidies shape tumour evolution and drug response
Authors: Shukla Ankit , Nguyen Thu H. M. , Moka Sarat B. , Ellis Jonathan J. , Grady John P. , Oey Harald , Cristino Alexandre S. , Khanna Kum Kum , Kroese Dirk P. , Krause Lutz , Dray Eloise , Fink J. Lynn , Duijf Pascal H. G. 
Published: January 2020

AbstractChromosome arm aneuploidies (CAAs) are pervasive in cancers. However, how they affect cancer development, prognosis and treatment remains largely unknown. Here, we analyse CAA profiles of 23,427 tumours, identifying aspects of tumour evolution including probable orders in which CAAs occur and CAAs predicting tissue-specific metastasis. Both haematological and solid cancers initially gain chromosome arms, while only solid cancers subsequently preferentially lose multiple arms. 72 CAAs and 88 synergistically co-occurring CAA pairs multivariately predict good or poor survival for 58% of 6977 patients, with negligible impact of whole-genome doubling. Additionally, machine learning identifies 31 CAAs that robustly alter response to 56 chemotherapeutic drugs across cell lines representing 17 cancer types. We also uncover 1024 potential synthetic lethal pharmacogenomic interactions. Notably, in predicting drug response, CAAs substantially outperform mutations and focal deletions/amplifications combined. Thus, CAAs predict cancer prognosis, shape tumour evolution, metastasis and drug response, and may advance precision oncology.
Wireless battery-free body sensor networks using near-field-enabled clothing
Authors: Lin Rongzhou , Kim Han-Joon , Achavananthadith Sippanat , Kurt Selman A. , Tan Shawn C. C. , Yao Haicheng , Tee Benjamin C. K. , Lee Jason K. W. , Ho John S. 
Published: January 2020

AbstractNetworks of sensors placed on the skin can provide continuous measurement of human physiological signals for applications in clinical diagnostics, athletics and human-machine interfaces. Wireless and battery-free sensors are particularly desirable for reliable long-term monitoring, but current approaches for achieving this mode of operation rely on near-field technologies that require close proximity (at most a few centimetres) between each sensor and a wireless readout device. Here, we report near-field-enabled clothing capable of establishing wireless power and data connectivity between multiple distant points around the body to create a network of battery-free sensors interconnected by proximity to functional textile patterns. Using computer-controlled embroidery of conductive threads, we integrate clothing with near-field-responsive patterns that are completely fabric-based and free of fragile silicon components. We demonstrate the utility of the networked system for real-time, multi-node measurement of spinal posture as well as continuous sensing of temperature and gait during exercise.
Context-sensitive adjustment of pointing in great apes
Authors: Tauzin Tibor , Bohn Manuel , Gergely György , Call Josep 
Published: January 2020

AbstractGreat apes are able to request objects from humans by pointing. It is unclear, however, whether this is an associated response to a certain set of cues (e.g. the presence and attention of a human addressee) or a communicative signal which can be adjusted to relevant aspects of the spatial and social context. In three experiments, we tested captive great apes’ flexible use of pointing gestures. We manipulated the communicative context so that the default pointing response of apes would have indicated an undesired object, either due to 1) the spatial arrangements of the target objects, 2) the perspective of the addressee or 3) the knowledge of the addressee about the target objects’ location. The results of the three experiments indicate that great apes can successfully adjust their pointing to the spatial configuration of the referent environment such as distance and location of food. However, we found no evidence that they take the perspective or the knowledge of the addressee into account when doing so. This implies that pointing in great apes is a context-sensitive, but maybe less versatile, communicative signal compared to human pointing.
A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum
Authors: Brennan K. , Martin K. , FitzGerald S. P. , O’Sullivan J. , Wu Y. , Blanco A. , Richardson C. , Mc Gee M. M. 
Published: January 2020

AbstractExtracellular vesicles (EVs) are nano-sized vesicles containing nucleic acid and protein cargo that are released from a multitude of cell types and have gained significant interest as potential diagnostic biomarkers. Human serum is a rich source of readily accessible EVs; however, the separation of EVs from serum proteins and non-EV lipid particles represents a considerable challenge. In this study, we compared the most commonly used isolation techniques, either alone or in combination, for the isolation of EVs from 200 µl of human serum and their separation from non-EV protein and lipid particles present in serum. The size and yield of particles isolated by each method was determined by nanoparticle tracking analysis, with the variation in particle size distribution being used to determine the relative impact of lipoproteins and protein aggregates on the isolated EV population. Purification of EVs from soluble protein was determined by calculating the ratio of EV particle count to protein concentration. Finally, lipoprotein particles co-isolated with EVs was determined by Western blot analysis of lipoprotein markers APOB and APOE. Overall, this study reveals that the choice of EV isolation procedure significantly impacts EV yield from human serum, together with the presence of lipoprotein and protein contaminants.
Diversity, compositional and functional differences between gut microbiota of children and adults
Authors: Radjabzadeh Djawad , Boer Cindy G. , Beth Sanne A. , van der Wal Pelle , Kiefte-De Jong Jessica C. , Jansen Michelle A. E. , Konstantinov Sergey R. , Peppelenbosch Maikel P. , Hays John P. , Jaddoe Vincent W. V. , Ikram M. Arfan , Rivadeneira Fernando , van Meurs Joyce B. J. , Uitterlinden André G. , Medina-Gomez Carolina , Moll Henriette A. , Kraaij Robert 
Published: January 2020

AbstractThe gut microbiota has been shown to play diverse roles in human health and disease although the underlying mechanisms have not yet been fully elucidated. Large cohort studies can provide further understanding into inter-individual differences, with more precise characterization of the pathways by which the gut microbiota influences human physiology and disease processes. Here, we aimed to profile the stool microbiome of children and adults from two population-based cohort studies, comprising 2,111 children in the age-range of 9 to 12 years (the Generation R Study) and 1,427 adult individuals in the range of 46 to 88 years of age (the Rotterdam Study). For the two cohorts, 16S rRNA gene profile datasets derived from the Dutch population were generated. The comparison of the two cohorts showed that children had significantly lower gut microbiome diversity. Furthermore, we observed higher relative abundances of genus Bacteroides in children and higher relative abundances of genus Blautia in adults. Predicted functional metagenome analysis showed an overrepresentation of the glycan degradation pathways, riboflavin (vitamin B2), pyridoxine (vitamin B6) and folate (vitamin B9) biosynthesis pathways in children. In contrast, the gut microbiome of adults showed higher abundances of carbohydrate metabolism pathways, beta-lactam resistance, thiamine (vitamin B1) and pantothenic (vitamin B5) biosynthesis pathways. A predominance of catabolic pathways in children (valine, leucine and isoleucine degradation) as compared to biosynthetic pathways in adults (valine, leucine and isoleucine biosynthesis) suggests a functional microbiome switch to the latter in adult individuals. Overall, we identified compositional and functional differences in gut microbiome between children and adults in a population-based setting. These microbiome profiles can serve as reference for future studies on specific human disease susceptibility in childhood, adulthood and specific diseased populations.
A Nondestructive Eggshell Thickness Measurement Technique Using Terahertz Waves
Authors: Khaliduzzaman Alin , Konagaya Keiji , Suzuki Tetsuhito , Kashimori Ayuko , Kondo Naoshi , Ogawa Yuichi 
Published: January 2020

AbstractEggshells play a number of important roles in the avian and reptile kingdom: protection of internal contents and as a major source of minerals for developing embryos. However, when researching these respective roles, eggshell thickness measurement remains a bottleneck due to the lack of a non-destructive measurement techniques. As a result, many avian and reptile research protocols omit consideration of eggshell thickness bias on egg or embryo growth and development. Here, we validate a non-destructive method to estimate eggshell thickness based on terahertz (THz) reflectance spectroscopy using chicken white coloured eggs. Since terahertz waves are reflected from outer air-eggshell interface, as well as the inner eggshell-membrane boundary, the resulting interference signals depend on eggshell thickness. Thus, it is possible to estimate shell thickness from the oscillation distance in frequency-domain. A linear regression-based prediction model for non-destructive eggshell thickness measurement was developed, which had a coefficient of determination (R2) of 0.93, RMSEP of 0.009, RPD of 3.45 and RER 13.67. This model can estimate eggshell thickness to a resolution of less than 10 μm. This method has the potential to expand the protocols in the field of avian and reptile research, as well as be applied to industrial grading of eggs.
Unique IL-13Rα2/STAT3 mediated IL-13 regulation detected in lung conventional dendritic cells, 24 h post viral vector vaccination
Authors: Roy Sreeja , Liu Ho-Ying , Jaeson Muhammad Irwan , Deimel Lachlan Paul , Ranasinghe Charani 
Published: January 2020

AbstractThis study demonstrates that 24 h following viral vector-based vaccination IL-13Rα2 functions as a master sensor on conventional dendritic cells (cDCs), abetted by high protein stability coupled with minimal mRNA expression, to rapidly regulate DC mediated IL-13 responses at the lung mucosae, unlike IL-13Rα1. Under low IL-13, IL-13Rα2 performs as a primary signalling receptor, whilst under high IL-13, acts to sequester IL-13 to maintain homeostasis, both in a STAT3-dependent manner. Likewise, we show that viral vector-derived IL-13 levels at the vaccination site can induce differential STAT3/STAT6 paradigms in lung cDC, that can get regulated collaboratively or independently by TGF-β1 and IFN-γ. Specifically, low IL-13 responses associated with recombinant Fowlpox virus (rFPV) is regulated by early IL-13Rα2, correlated with STAT3/TGF-β1 expression. Whilst, high IL-13 responses, associated with recombinant Modified Vaccinia Ankara (rMVA) is regulated in an IL-13Rα1/STAT6 dependent manner associated with IFN-γR expression bias. Different viral vaccine vectors have previously been shown to induce unique adaptive immune outcomes. Taken together current observations suggest that IL-13Rα2-driven STAT3/STAT6 equilibrium at the cDC level may play an important role in governing the efficacy of vector-based vaccines. These new insights have high potential to be exploited to improve recombinant viral vector-based vaccine design, according to the pathogen of interest and/or therapies against IL-13 associated disease conditions.
Sodium-induced population shift drives activation of thrombin
Authors: Kahler Ursula , Kamenik Anna S. , Kraml Johannes , Liedl Klaus R. 
Published: January 2020

AbstractThe equilibrium between active E and inactive E* forms of thrombin is assumed to be governed by the allosteric binding of a Na+ ion. Here we use molecular dynamics simulations and Markov state models to sample transitions between active and inactive states. With these calculations we are able to compare thermodynamic and kinetic properties depending on the presence of Na+. For the first time, we directly observe sodium-induced conformational changes in long-timescale computer simulations. Thereby, we are able to explain the resulting change in activity. We observe a stabilization of the active form in presence of Na+ and a shift towards the inactive form in Na+-free simulations. We identify key structural features to quantify and monitor this conformational shift. These include the accessibility of the S1 pocket and the reorientation of W215, of R221a and of the Na+ loop. The structural characteristics exhibit dynamics at various timescales: Conformational changes in the Na+ binding loop constitute the slowest observed movement. Depending on its orientation, it induces conformational shifts in the nearby substrate binding site. Only after this shift, residue W215 is able to move freely, allowing thrombin to adopt a binding-competent conformation.
Silencing E3 Ubiqutin ligase ITCH as a potential therapy to enhance chemotherapy efficacy in p53 mutant neuroblastoma cells
Authors: Meng Jinhong , Tagalakis Aristides D. , Hart Stephen L. 
Published: January 2020

AbstractP53 mutations are responsible for drug-resistance of tumour cells which impacts on the efficacy of treatment. Alternative tumour suppressor pathways need to be explored to treat p53- deficient tumours. The E3 ubiquitin ligase, ITCH, negatively regulates the tumour suppressor protein TP73, providing a therapeutic target to enhance the sensitivity of the tumour cells to the treatment. In the present study, two p53-mutant neuroblastoma cell lines were used as in vitro models. Using immunostaining, western blot and qPCR methods, we firstly identified that ITCH was expressed on p53-mutant neuroblastoma cell lines. Transfection of these cell lines with ITCH siRNA could effectively silence the ITCH expression, and result in the stabilization of TP73 protein, which mediated the apoptosis of the neuroblastoma cells upon irradiation treatment. Finally, in vivo delivery of the ITCH siRNA using nanoparticles to the neuroblastoma xenograft mouse model showed around 15–20% ITCH silencing 48 hours after transfection. Our data suggest that ITCH could be silenced both in vitro and in vivo using nanoparticles, and silencing of ITCH sensitizes the tumour cells to irradiation treatment. This strategy could be further explored to combine the chemotherapy/radiotherapy treatment to enhance the therapeutic effects on p53-deficient neuroblastoma.
Teratological cases of the antennae in the family Aradidae (Hemiptera: Heteroptera)
Authors: Taszakowski Artur , Kaszyca-Taszakowska Natalia 
Published: January 2020

AbstractTeratological cases of the antennae in the family Aradidae (Hemiptera: Heteroptera) are widely described for the first time. Four hundred seventy-six specimens of flat bugs were studied, and antennal malformations were found in 14 of them (2.94%) (belonging to eight species and three subfamilies: Aradinae, Aneurinae and Mezirinae). All of the teratologies were observed using optical microscopy; moreover, in order to determine any compensatory regeneration, selected cases were also studied using a scanning electron microscope. In almost all of the specimens, the successful regeneration of the sensory organs to various degrees was observed. Additional results were the discovery of a previously unrecognized type of sensillum in flat bugs – a campaniform sensillum as well as significant differences in the distribution of the sensilla depending on the systematic affiliation.
Variation in chemokines plasma concentrations in primary care depressed patients associated with Internet-based cognitive-behavioral therapy
Authors: Romero-Sanchiz Pablo , Nogueira-Arjona Raquel , Araos Pedro , Serrano Antonia , Barrios Vicente , Argente Jesús , Garcia-Marchena Nuria , Lopez-Tellez Antonio , Rodriguez-Moreno Silvia , Mayoral Fermin , Pavón Francisco J. , Fonseca Fernando Rodríguez de 
Published: January 2020

AbstractHow the presence of inflammation has repercussions for brain function is a topic of active research into depression. Signals released from immune system-related cells, including chemokines, might be indicative of active depression and can, hypothetically, serve as biomarkers of response to interventions, both pharmacological and psychological. The objective of this study is to analyze the peripheral plasma concentrations of CXCL12, CCL11, CX3CL1 and CCL2 in a cohort of depressed primary-care patients, as well as their evolution after an internet-based cognitive-behavioral intervention. The concentrations of those chemokines were measured in 66 primary-care patients with mild and moderate depression, before and after the intervention, as well as 60 controls, using multiplex immunoassays. Concentrations of CXCL12 and CCL2 were significantly higher in the clinical sample in comparison with controls. A stable multivariate discriminative model between both groups was found. Concentrations of all chemokines decreased after the internet-based psychological intervention. These findings support the implication of chemokines in depression, even in a sample of patients with mild and moderate severity. Furthermore, they demonstrate the need for further multidisciplinary research that confirms how biomarkers such as plasma chemokines can serve as a marker for depression and are sensitive to non-pharmacological interventions.
Altered dorsal CA1 neuronal population coding in the APP/PS1 mouse model of Alzheimer’s disease
Authors: Chockanathan Udaysankar , Warner Emily J. , Turpin Loel , O’Banion M. Kerry , Padmanabhan Krishnan 
Published: January 2020

AbstractWhile the link between amyloid β (Aβ) accumulation and synaptic degradation in Alzheimer’s disease (AD) is known, the consequences of this pathology on population coding remain unknown. We found that the entropy, a measure of the diversity of network firing patterns, was lower in the dorsal CA1 region in the APP/PS1 mouse model of Aβ pathology, relative to controls, thereby reducing the population’s coding capacity. Our results reveal a network level signature of the deficits Aβ accumulation causes to the computations performed by neural circuits.
Embryo survival in the oviduct not significantly influenced by major histocompatibility complex social signaling in the horse
Authors: Jeannerat E. , Marti E. , Thomas S. , Herrera C. , Sieme H. , Wedekind C. , Burger D. 
Published: January 2020

AbstractThe major histocompatibility complex (MHC) influences sexual selection in various vertebrates. Recently, MHC-linked social signaling was also shown to influence female fertility in horses (Equus caballus) diagnosed 17 days after fertilization. However, it remained unclear at which stage the pregnancy was terminated. Here we test if MHC-linked cryptic female choice in horses happens during the first days of pregnancy, i.e., until shortly after embryonic entrance into the uterus and before fixation in the endometrium. We exposed estrous mares to one of several unrelated stallions, instrumentally inseminated them with semen of another stallion, and flushed the uterus 8 days later to test for the presence of embryos. In total 68 embryos could be collected from 97 experimental trials. This success rate of 70.1% was significantly different from the mean pregnancy rate of 45.7% observed 17 days after fertilization using the same experimental protocol but without embryo flushing. Embryo recovery rate was not significantly dependent on whether the mares had been socially exposed to an MHC-dissimilar or an MHC-similar stallion. These observations suggest that MHC-linked maternal strategies affect embryo survival mainly (or only) during the time of fixation in the uterus.
An intra-bacterial activity for a T3SS effector
Authors: El Qaidi Samir , Scott Nichollas E. , Hays Michael P. , Geisbrecht Brian V. , Watkins Shelby , Hardwidge Philip R. 
Published: January 2020

AbstractMany Gram-negative bacterial pathogens interact with mammalian cells by using type III secretion systems (T3SS) to inject virulence proteins into host cells. A subset of these injected protein ‘effectors’ are enzymes that inhibit the function of host proteins by catalyzing the addition of unusual post-translational modifications. The E. coli and Citrobacter rodentium NleB effectors, as well as the Salmonella enterica SseK effectors are glycosyltransferases that modify host protein substrates with N-acetyl glucosamine (GlcNAc) on arginine residues. This post-translational modification disrupts the normal functioning of host immune response proteins. T3SS effectors are thought to be inactive within the bacterium and fold into their active conformations after they are injected, due to the activity of chaperones that keep the effectors in a structural state permissive for secretion. While performing mass spectrometry experiments to identify glycosylation substrates of NleB orthologs, we unexpectedly observed that the bacterial glutathione synthetase (GshB) is glycosylated by NleB on arginine residue R256. NleB-mediated glycosylation of GshB resulted in enhanced GshB activity, leading to an increase in glutathione production, and promoted C. rodentium survival in oxidative stress conditions. These data represent, to our knowledge, the first intra-bacterial activity for a T3SS effector and show that arginine-GlcNAcylation, once thought to be restricted to host cell compartments, also plays an important role in regulating bacterial physiology.