bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Cortico-Collicular Amplification Mechanism for Gap Detection
Authors: Weible Aldis P , Yavorska Iryna , Wehr Michael 
Published: December 2019

Abstract
Auditory cortex (AC) is necessary for the detection of brief gaps in ongoing sounds, but not for the detection of longer gaps or other stimuli such as tones or noise. It remains unclear why this is so, and what is special about brief gaps in particular. Here, we used both optogenetic suppression and conventional lesions to show that the cortical dependence of brief gap detection hinges specifically on gap termination. We then identified a cortico-collicular gap detection circuit that amplifies cortical gap termination responses before projecting to inferior colliculus (IC) to impact behavior. We found that gaps evoked off-responses and on-responses in cortical neurons, which temporally overlapped for brief gaps, but not long gaps. This overlap specifically enhanced cortical responses to brief gaps, whereas IC neurons preferred longer gaps. Optogenetic suppression of AC reduced collicular responses specifically to brief gaps, indicating that under normal conditions, the enhanced cortical representation of brief gaps amplifies collicular gap responses. Together these mechanisms explain how and why AC contributes to the behavioral detection of brief gaps, which are critical cues for speech perception, perceptual grouping, and auditory scene analysis.
Long-term Outcome of Patients With Nonoperated Prosthetic Valve Infective Endocarditis: Is Relapse the Main Issue?
Authors: Lecomte Raphaël , Laine Jean-Baptiste , Issa Nahéma , Revest Matthieu , Gaborit Benjamin , Le Turnier Paul , Deschanvres Colin , Benezit François , Asseray Nathalie , Le Tourneau Thierry , Pattier Sabine , Al Habash Ousama , Raffi François , Boutoille David , Camou Fabrice 
Published: December 2019

Abstract
In nonoperated prosthetic valve endocarditis (PVE), long-term outcome is largely unknown. We report the follow-up of 129 nonoperated patients with PVE alive at discharge. At 1 year, the mortality rate was 24%; relapses and reinfection were rare (5% each). Enterococcal PVE was associated with a higher risk of relapse.
NADPH oxidase-4 promotes eccentric cardiac hypertrophy in response to volume overload
Authors: Schnelle Moritz , Sawyer Iain , Anilkumar Narayana , Mohamed Belal A , Richards Daniel A , Toischer Karl , Zhang Min , Catibog Norman , Sawyer Greta , Mongue-Din Héloïse , Schröder Katrin , Hasenfuss Gerd , Shah Ajay M 
Published: December 2019

Abstract

Aims
Chronic pressure or volume overload induce concentric vs. eccentric left ventricular (LV) remodelling, respectively. Previous studies suggest that distinct signalling pathways are involved in these responses. NADPH oxidase-4 (Nox4) is a reactive oxygen species-generating enzyme that can limit detrimental cardiac remodelling in response to pressure overload. This study aimed to assess its role in volume overload-induced remodelling.

Methods and results
We compared the responses to creation of an aortocaval fistula (Shunt) to induce volume overload in Nox4-null mice (Nox4−/−) vs. wild-type (WT) littermates. Induction of Shunt resulted in a significant increase in cardiac Nox4 mRNA and protein levels in WT mice as compared to Sham controls. Nox4−/− mice developed less eccentric LV remodelling than WT mice (echocardiographic relative wall thickness: 0.30 vs. 0.27, P &lt; 0.05), with less LV hypertrophy at organ level (increase in LV weight/tibia length ratio of 25% vs. 43%, P &lt; 0.01) and cellular level (cardiomyocyte cross-sectional area: 323 µm2 vs. 379 μm2, P &lt; 0.01). LV ejection fraction, foetal gene expression, interstitial fibrosis, myocardial capillary density, and levels of myocyte apoptosis after Shunt were similar in the two genotypes. Myocardial phospho-Akt levels were increased after induction of Shunt in WT mice, whereas levels decreased in Nox4−/− mice (+29% vs. −21%, P &lt; 0.05), associated with a higher level of phosphorylation of the S6 ribosomal protein (S6) and the eIF4E-binding protein 1 (4E-BP1) in WT compared to Nox4−/− mice. We identified that Akt activation in cardiac cells is augmented by Nox4 via a Src kinase-dependent inactivation of protein phosphatase 2A.

Conclusion
Endogenous Nox4 is required for the full development of eccentric cardiac hypertrophy and remodelling during chronic volume overload. Nox4-dependent activation of Akt and its downstream targets S6 and 4E-BP1 may be involved in this effect.
Echocardiographic phenotype and prognosis in transthyretin cardiac amyloidosis
Authors: Chacko Liza , Martone Raffaele , Bandera Francesco , Lane Thirusha , Martinez-Naharro Ana , Boldrini Michele , Rezk Tamer , Whelan Carol , Quarta Cristina , Rowczenio Dorota , Gilbertson Janet A , Wongwarawipat Tanakal , Lachmann Helen , Wechalekar Ashutosh , Sachchithanantham Sajitha , Mahmood Shameem , Marcucci Rossella , Knight Daniel , Hutt David , Moon James , Petrie Aviva , Cappelli Francesco , Guazzi Marco , Hawkins Philip N , Gillmore Julian D , Fontana Marianna 
Published: December 2019

Abstract

Aims
Transthyretin amyloidosis cardiomyopathy (ATTR-CM) is an increasingly recognized cause of heart failure. We sought to characterize the structural and functional echocardiographic phenotype across the spectrum of wild-type (wtATTR-CM) and hereditary (hATTR-CM) transthyretin cardiomyopathy and the echocardiographic features predicting prognosis.

Methods and results
We studied 1240 patients with ATTR-CM who underwent prospective protocolized evaluations comprising full echocardiographic assessment and survival between 2000 and 2019, comprising 766 with wtATTR-CM and 474 with hATTR-CM, of whom 314 had the V122I variant and 127 the T60A variant. At diagnosis, patients with V122I-hATTR-CM had the most severe degree of systolic and diastolic dysfunction across all echocardiographic parameters and patients with T60AhATTR-CM the least; patients with wtATTR-CM had intermediate features. Stroke volume index, right atrial area index, longitudinal strain, and E/e’ were all independently associated with mortality (P &lt; 0.05 for all). Severe aortic stenosis (AS) was also independently associated with prognosis, conferring a significantly shorter survival (median survival 22 vs. 53 months, P = 0.001).

Conclusion
The three distinct genotypes present with varying degrees of severity. Echocardiography indicates a complex pathophysiology in which both systolic and diastolic function are independently associated with mortality. The presence of severe AS was independently associated with significantly reduced patient survival.
Expanding the capillary electrophoresis-based glucose unit database of the GUcal app
Authors: Jarvas Gabor , Szigeti Marton , Campbell Matthew P , Guttman Andras 
Published: December 2019

Abstract
GUcal is a standalone application for automatically calculating the glucose unit (GU) values for separated N-glycan components of interest in an electropherogram and suggests their tentative structures by utilizing an internal database. We have expanded the original database of GUcal by integrating all publicly available capillary electrophoresis (CE) data in the GlycoStore collection (https://www.glycostore.org) and with in-house measured GU values. The GUcal app is freely available online (https://www.gucal.hu) and readily facilitates CE-based high throughput GU value determination for first line structural elucidation.
Heparan sulfate inhibits transforming growth factor β signaling and functionsin cisandin transto regulate prostate stem/progenitor cell activities
Authors: Rai Sumit , Alsaidan Omar Awad , Yang Hua , Cai Houjian , Wang Lianchun 
Published: December 2019

AbstractProstate stem/progenitor cells (PrSCs) are responsible for adult prostate tissue homeostasis and regeneration. However, the related regulatory mechanisms are not completely understood. In this study, we examined the role of heparan sulfate (HS) in PrSC self-renewal and prostate regeneration. Using an in vitro prostate sphere formation assay, we found that deletion of the glycosyltransferase exostosin 1 (Ext1) abolished HS expression in PrSCs and disrupted their ability to self-renew. In associated studies, we observed that HS loss inhibited p63 and CK5 expression, reduced the number of p63+- or CK5+-expressing stem/progenitor cells, elevated CK8+ expression and the number of differentiated CK8+ luminal cells and arrested the spheroid cells in the G1/G0 phase of cell cycle. Mechanistically, HS expressed by PrSCs (in cis) or by neighboring cells (in trans) could maintain sphere formation. Furthermore, HS deficiency upregulated transforming growth factor β (TGFβ) signaling and inhibiting TGFβ signaling partially restored the sphere-formation activity of the HS-deficient PrSCs. In an in vivo prostate regeneration assay, simultaneous loss of HS in both epithelial cell and stromal cell compartments attenuated prostate tissue regeneration, whereas the retention of HS expression in either of the two cellular compartments was sufficient to sustain prostate tissue regeneration. We conclude that HS preserves self-renewal of adult PrSCs by inhibiting TGFβ signaling and functions both in cis and in trans to maintain prostate homeostasis and to support prostate regeneration.
Humpback whales extend their stay in a breeding ground in the Tropical Eastern Pacific
Authors: Avila Isabel Cristina , Dormann Carsten F , García Carolina , Payán Luis Fernando , Zorrilla María Ximena 
Published: December 2019

AbstractDuring the austral winter, G-stock humpback whales, Megaptera novaeangliae, migrate to the Tropical Eastern Pacific to breed. To analyse if the whale migration times have changed over time, we analysed 31 years (1988–2018) of arrival and departure times to Gorgona National Park, Colombia, an important breeding site. During this period, whales have significantly changed their arrival time, coming now earlier, but their departure time has not changed significantly. Hence, humpback whales now stay 1 month longer than 31 years ago. Humpbacks arrived in Gorgona at the earliest during the beginning of May and stayed at the most until late December. The change observed in the arrival time to breeding grounds could be related to ice sheet mass changes in autumn in Antarctica and increase in population size over the past decades but we were unable to determine which factor is more important in explaining the observed trend. Management decisions in Colombia need to account for a longer stay, specifically restricting anthropogenic activities from 1May to 31December. We urge other researchers to review their data, in case this shift is evident in other regions and management plans need to be updated.
Vibration of effects in epidemiologic studies of alcohol consumption and breast cancer risk
Authors: Chu Lingzhi , Ioannidis John P A , Egilman Alex C , Vasiliou Vasilis , Ross Joseph S , Wallach Joshua D 
Published: December 2019

AbstractBackgroundDifferent analytical approaches can influence the associations estimated in observational studies. We assessed the variability of effect estimates reported within and across observational studies evaluating the impact of alcohol on breast cancer.MethodsWe abstracted largest harmful, largest protective and smallest (closest to the null value of 1.0) relative risk estimates in studies included in a recent alcohol–breast cancer meta-analysis, and recorded how they differed based on five model specification characteristics, including exposure definition, exposure contrast levels, study populations, adjustment covariates and/or model approaches. For each study, we approximated vibration of effects by dividing the largest by the smallest effect estimate [i.e. ratio of odds ratio (ROR)].ResultsAmong 97 eligible studies, 85 (87.6%) reported both harmful and protective relative effect estimates for an alcohol–breast cancer relationship, which ranged from 1.1 to 17.9 and 0.0 to 1.0, respectively. The RORs comparing the largest and smallest estimates in value ranged from 1.0 to 106.2, with a median of 3.0 [interquartile range (IQR) 2.0–5.2]. One-third (35, 36.1%) of the RORs were based on extreme effect estimates with at least three different model specification characteristics; the vast majority (87, 89.7%) had different exposure definitions or contrast levels. Similar vibrations of effect were observed when only extreme estimates with differences based on study populations and/or adjustment covariates were compared.ConclusionsMost observational studies evaluating the impact of alcohol on breast cancer report relative effect estimates for the same associations that diverge by &gt;2-fold. Therefore, observational studies should estimate the vibration of effects to provide insight regarding the stability of findings.
Interfaces for collecting data from patients: 10 golden rules
Authors: Vickers Andrew J , Chen Ling Y , Stetson Peter D 
Published: December 2019

Abstract
Memorial Sloan Kettering Cancer Center has more than a decade's experience creating online interfaces for obtaining data from patients as part of routine clinical care. We have developed a set of “golden rules” for design of these interfaces. Many relate to the knowledge imbalance between professional staff (whether medical or informatics) and patients, who are often old and sick and have limited knowledge of technology. Others relate to the clinical nature of the encounter: data cannot be taken from patients as part of clinical care unless there is a plan to act on whatever information is prepared. We also note that the plethora of marketing questionnaires makes patients suspicious of surveys: patient trust is hard to gain and easy to lose. Addition of these golden rules to standard approaches to interface design will maximize our ability to obtain data from patients and thus improve communication between patients and clinicians.
301 Effects of housing system on performance and behavior of dairy calves before and after weaning
Authors: Liu Shuai , Alugongo Gibson , Li Jinghui , Cao Zhijun , Wang Yajing , Li Shengli 
Published: December 2019

Abstract
The aim of this study was to evaluate the effects of housing system on performance and behavior of dairy calves during pre-weaning (d 1–42), weaning (d 43–56), post-weaning (d 57–63) and mixing (d 64–70) period. Thirty-seven newborn Holstein dairy calves were allocated to individual (n = 17) or pair housing (n = 10 pairs). After that, calves remained in their pens until d 63 ± 1, and then PH group and IH group were mixed and moved to 6 group pens randomly. Starter intake, fecal consistency scores and body weight were collected. Instantaneous scan-sampling with 5-min intervals was used to collect the behavioral observations (lying, ruminating, standing, eating, drinking, walking, self-grooming, manipulating object) for 48 h beginning on d 43, 50, 57 and the second day after mixing. Data were analyzed by mixed effect model with treatment, time, and the interaction between them as fixed effects, and animal within treatment as a random effect. During post-weaning period, PH group had higher starter intake (P = 0.02) and average daily gain (P = 0.02) than IH group. PH group weighed more on d 63 (P = 0.002) and d 70 (P = 0.002). PH group had higher diarrhea frequency than IH group in week 3 (P = 0.03). PH increased lying time in week 8 (P = 0.03) and walking time during post-weaning period (P = 0.05), yet IH group spent more time standing in week 8 (P = 0.008), eating during post-weaning period (P = 0.008), self-grooming during weaning period (week 7, P = 0.007; week 8, P = 0.03). Furthermore, previous housing system had no effect on calves’ behavior after mixing. In conclusion, Pair housing during post-weaning period improved performance of calves and housing system had on effect on behavior after mixing.

Figure 1. ADG (mean±SEM) for calves housed individually (n = 17 calves) or in pairs (n = 10 pairs). PH = calves housed in pairs; IH = calves housed individually. * P ≤ 0.05, † P &lt; 0.10. Figure 2. BW (mean±SEM) for calves housed individually (n = 17 calves) or in pairs (n = 10 pairs). PH = calves housed in pairs; IH = calves housed individually. * P ≤ 0.05, † P &lt; 0.10. Figure 3. Diarrhea frequency for calves housed individually (n = 17 calves) or in pairs (n = 10 pairs) before 63d. PH = calves housed in pairs; IH = calves housed individually. * P ≤ 0.05. Figure 4. Time (mean±SEM) for calves (n = 6) performing each behavior as affected by different housing system during weaning, post-weaning and mixing period.* P ≤ 0.05.
304 Transgenerational influence of prenatal stress on temperament, birth weight, and weaning weight of Brahman calves
Authors: Littlejohn Brittni P , Riley David G , Neuendorff Don A , Long Charles R , Welsh Thomas H , Randel Ronald D 
Published: December 2019

Abstract
The objective was to evaluate the transgenerational influence of prenatal transportation stress (PNS) on temperament, birth weight, and weaning weight in Brahman calves. Dams were derived from multiparous cows that were transported for 2 h at 60, 80, 100, 120, and 140 d of gestation (n = 48) or maintained as Controls (n = 48). Heifers born to Transported or Control cows were followed through maturity and their calves (n = 79; 35 calves born to Control dams and 44 to PNS dams) studied across multiple years. Calf birth and weaning weights were recorded. Calf temperament at weaning was evaluated utilizing pen score (PS; 1=calm and 5=excitable), exit velocity (EV; m/sec), and temperament score (TS; (PS+EV)/2). Data were analyzed using mixed linear models. Fixed effects included dam treatment, calf sex, and birth season nested within birth year (season nested within year was identified as an efficient parameterization). Calf sire was a random effect. Birth weight was not affected by dam treatment, calf sex, or season nested within year (P &gt; 0.1). Weaning weight was greater in males than females (P &lt; 0.01). Weaning weight was affected by season nested within year (P &lt; 0.01). DOA at weaning was a covariate for weaning weight (0.74±0.09 kg; P &lt; 0.01), but not PS, EV, or TS (P &lt; 0.1). Weaning weight was not affected by dam treatment (P &gt; 0.1). TS was affected by season nested within year (P = 0.04) but not dam treatment or calf sex (P &gt; 0.1). EV was not affected by dam treatment, calf sex, or season nested within year (P &gt; 0.1). PS was affected by season nested within year (P &lt; 0.01) but not calf sex (P &gt; 0.1). Calves born to PNS dams had greater (P = 0.02) PS than calves born to Controls (3.17±0.20 and 2.58±0.22, respectively). There was a transgenerational influence of PNS on temperament but not birth or weaning weight.
320 Carcass characteristics of Bos indicus-Bos taurus steers following BVDV type 1b challenge
Authors: Knowles Arthur , Paschal Joe , Runyan Chase , Sawyer Jason , Herring Andy 
Published: December 2019

Abstract
Bovine Viral Diarrhea virus (BVDV), is a component of bovine respiratory disease (BRD) worldwide. Documentation of animal genetic influences and performance variability specific to BVDV challenge is limited. This study evaluated individual animal effects in the 14 d following BVDV Type 1b intranasal challenge in yearling, Nellore-Angus (Bos indicus-Bos taurus) F2 and F3 crossbred steers (n = 363) on carcass traits 180 d post-challenge. Prior to challenge steers were confirmed to be free of BVDV infection with families balanced across non-vaccinated control, killed (KV), or modified-live (MLV) commercial BRD vaccines. Hot carcass weight (HCW), adjusted fat thickness (AFT), longissimus muscle area (REA) and marbling score (MARB) were analyzed through mixed models with sire and pen(year) as random effects; potential fixed effects of vaccine, type of cross, pyrexia status, clinical sign presentation, and levels of feed intake and ADG 14 d post-challenge were investigated. Vaccine influenced AFT (P = 0.016); MLV steers had 0.26 cm less AFT than KV steers. Marbling was affected by type of cross (P = 0.023) with up to 0.60 marbling scores higher (P &lt; 0.05) in some parental combinations; an interaction between type of cross and pyrexia status also affected MARB (P = 0.017) with one parental combination having higher MARB associated with pyrexia. Hot carcass weight was not affected by pyrexia, but was affected by feed intake (P = 0.019), with steers in the highest vs. lowest category averaging 24.0 kg heavier. No animals presented morbidity signs severe enough for therapeutic treatment, yet presence of mild clinical symptoms affected REA (P = 0.012), resulting in 3.4 cm2 lower REA, however, this pattern was inconsistent among feed intake levels. This study affirms the complexity of health impacts on beef carcass traits and the need for improved study of subclinical illness in beef production systems.
318 Total flavonoids from Rhizoma drynariae ameliorate low calcium-induced osteoporosis by regulating the bone metabolism in caged laying hens
Authors: Huang Jie , Zhang Lei , Zhou Zhongxin 
Published: December 2019

Abstract
Cage layer osteoporosis (CLO) is one of the most serious bone metabolic diseases in laying hens industry. This disease not only causes huge economic losses, but also greatly affects the welfare of animals. Traditional Chinese medicines are widely used for prevention and treatment of all kinds of diseases. It is famous for excellent treatment, low side effects, and wide range of use safety. Total flavonoids from Rhizoma drynariae (TFRD) is an herbal product extracted from the dried root of Rhizoma drynariae. Recently, many researcher reports about the beneficial effects of TFRD in relation to various types of bone conditions but no report is available about promoting effect of TFRD against CLO. Therefore, current study was conducted to explore the ameliorating effect of TFRD in low calcium-induced osteoporosis. A total of 144 Lohmann pink-shell laying hens of 31-week-old were equally distributed in four groups: The control group (containing calcium 3.5% in based diet), the low calcium group (containing calcium 1.5% in low-calcium diet), the test group 1 (adding 500 mg/kg TFRD to low-calcium diet), the test group 2 (adding 2000 mg/kg TFRD to low-calcium diet). The birds were fed the diets for 60 d. The bone mineral density of tibia was measured by dual-energy X-ray absorptiometry indicating that the low calcium group had lower bone density than the control group. Compared with the low calcium group, the supplementation of TFRD protects bone tissue and prevents bone density from decreasing. Serum biochemical analysis showed that TFRD supplementation decreased the level of alkaline phosphatase, tartrate-resistant acid phosphatase, osteocalcin and calcitonin. Therefore, adding TFRD to low-calcium diet can improve the activity of important biochemical markers, clearly inhibit bone resorption and alleviate osteoporosis induce by low-calcium diet in caged laying hens.