bluuebunny/crossref_metadata_2025_split huggingface dataset:
MS-based proteomic analysis of cardiac response to hypoxia in the goldfish (Carassius auratus)
Authors: Imbrogno Sandra , Aiello Donatella , Filice Mariacristina , Leo Serena , Mazza Rosa , Cerra Maria Carmela , Napoli Anna 
Published: December 2019

AbstractThe exceptional hypoxia tolerance of the goldfish heart may be achieved through the activation of an alternative mechanism recruiting the first product of the anaerobic glycolysis (i.e. piruvate). This hypothesis led to design a classical mass spectrometry based proteomic study to identify in the goldfish cardiac proteins that may be associated with maintaining heart function under normoxia and hypoxia. A selective protein solubilization, SDS PAGE, trypsin digestion and MALDI MS/MS analysis allowed the identification of the 12 most stable hypoxia-regulated proteins. Among these proteins, five are enzymes catalyzing reversible steps of the glycolysis/gluconeogenesis network. Protein composition reveals the presence of fructose-1,6-bisphosphate aldolase B as a specific hypoxia-regulated protein. This work indicated that the key enzyme of reversible steps of the glycolysis/gluconeogenesis network is fructose-1,6-bisphosphate, aldolase B, suggesting a role of gluconeogenesis in the mechanisms involved in the goldfish heart response to hypoxia.
Direct Observation of Deformation in Microgel Filtration
Authors: Linkhorst John , Rabe Jonas , Hirschwald Lukas T. , Kuehne Alexander J. C. , Wessling Matthias 
Published: December 2019

AbstractColloidal filtration processes using porous membranes suffer from productivity loss due to colloidal matter retention and continuous build-up by the retained matter. Especially during filtration of soft matter, the deformation of the individual colloids that make up the filter cake may be significant; however, this deformation and its impact remain unresolved so far. Yet, understanding the deformation on the single colloid level as well as on the ensemble level is important to be able to deconvolute filter cake properties from resistance increase of the membrane either by simultaneous internal adsorption or blocking of pores. Here, we report on the compression of a filter cake by filtrating soft microgels in a microfluidic channel in front of a model membrane. To study the single colloid deformation amorphous and crystalline domains were built up in front of the membrane and visualized on-line using confocal fluorescence microscopy while adjusting the degree of permeation, i.e., the transmembrane flux. Results show locally pronounced asymmetric deformation in amorphous domains, while the microgels in colloidal crystals approached regular polyeder shape. Increasing the flux beyond the maximum colloid deformation results in non-isochoric microgel behavior. The presented methodology enables a realistic description of complex colloidal matter deposits during filtration.
RNA-binding protein FXR1 is presented in rat brain in amyloid form
Authors: Sopova Julia V. , Koshel Elena I. , Belashova Tatiana A. , Zadorsky Sergey P. , Sergeeva Alexandra V. , Siniukova Vera A. , Shenfeld Alexandr A. , Velizhanina Maria E. , Volkov Kirill V. , Nizhnikov Anton A. , Kachkin Daniel V. , Gaginskaya Elena R. , Galkin Alexey P. 
Published: December 2019

AbstractAmyloids are β-sheets-rich protein fibrils that cause neurodegenerative and other incurable human diseases affecting millions of people worldwide. However, a number of proteins is functional in the amyloid state in various organisms from bacteria to humans. Using an original proteomic approach, we identified a set of proteins forming amyloid-like aggregates in the brain of young healthy rats. One of them is the FXR1 protein, which is known to regulate memory and emotions. We showed that FXR1 clearly colocalizes in cortical neurons with amyloid-specific dyes Congo-Red, Thioflavines S and T. FXR1 extracted from brain by immunoprecipitation shows yellow-green birefringence after staining with Congo red. This protein forms in brain detergent-resistant amyloid oligomers and insoluble aggregates. RNA molecules that are colocalized with FXR1 in cortical neurons are insensitive to treatment with RNase A. All these data suggest that FXR1 functions in rat brain in amyloid form. The N-terminal amyloid-forming fragment of FXR1 is highly conserved across mammals. We assume that the FXR1 protein may be presented in amyloid form in brain of different species of mammals, including humans.
Characterization of vaginal microbiota in women with preterm labor with intra-amniotic inflammation
Authors: Cobo Teresa , Vergara Andrea , Collado Maria Carmen , Casals-Pascual Climent , Herreros Eduardo , Bosch Jordi , Sánchez-García Ana B. , López-Parellada Rosa , Ponce Júlia , Gratacós Eduard 
Published: December 2019

AbstractThis study aims to investigate the relation between vaginal microbiota and exposition to intra-amniotic inflammation (IAI). We conducted a prospective cohort study in women with preterm labor <34 weeks who had undergone amniocentesis to rule out IAI. Vaginal samples were collected after amniocentesis. Women with IAI included those with positive amniotic fluid (AF) for a microorganism identified by specific culture media and Sanger sequencing 16S ribosomal RNA gene and/or high AF interleukin (IL)-6 levels. Vaginal microbiota was characterized by 16S ribosomal RNA gene amplicon sequencing. Specific quantitative PCR targeted to Lactobacillus spp. was also performed. Regression models were used to evaluate associations between vaginal microbiota and exposition to IAI. Concerning our results, 64 women were included. We observed an inverse association between AF IL-6 levels and load of Lactobacillus spp. Depletion in Lactobacillus spp. load was significantly associated with an early gestational age at delivery and a short latency to delivery. Microbial-diversity was found to be a risk factor for the subsequent occurrence of clinical chorioamnionitis. To the contrary, higher Lactobacillus spp. load had a protective role. In conclusion, the study identifies reduced bacterial load of Lactobacillus spp. in women exposed to IAI and found microbial-diversity and Lactobacillus spp. depletion to be associated with a worse perinatal outcome.
Effects of Bimodal and Bilateral Cochlear Implant Use on a Nonauditory Working Memory Task: Reading Span Tests Over 2 Years Following Cochlear Implantation
Authors: Macpherson Ewan A. , Curca Ioan A. , Scollie Susan , Parsa Vijay , Vansevenant Katherine , Zimmerman Kim , Lewis-Teeter Jamie , Allen Prudence , Parnes Lorne , Agrawal Sumit 
Published: December 2019

Purpose
A growing body of evidence indicates that treatment of hearing loss by provision of hearing aids leads to improvements in auditory and visual working memory. The purpose of this study was to assess whether similar working memory benefits are observed following provision of cochlear implants (CIs).

Method
Fifteen adults with postlingually acquired severe bilateral sensorineural hearing loss completed the prospective longitudinal study. Participants were candidates for bilateral cochlear implantation with some aidable hearing in each ear. Implantation surgeries were carried out sequentially, approximately 1 year apart. Working memory was measured with the visual Reading Span Test (Daneman & Carpenter, 1980) at 5 time points: pre-operatively following a 6-month bilateral hearing aid trial, after 6 and 12 months of bimodal (CI plus contralateral hearing aid) listening experience following the 1st CI surgery and activation, and again after 6 and 12 months of bilateral CI listening experience following the 2nd CI surgery and activation.

Results
Compared to the preoperative baseline, CI listening experience yielded significant improvements in participants' ability to recall test words in the correct serial order after 12 months in the bimodal condition. Individual performance outcomes were variable, but almost all participants showed increases in task performance over the course of the study.

Conclusions
These results suggest that, similar to appropriate interventions with hearing aids, treatment of hearing loss with CIs can yield working memory benefits. A likely mechanism is the freeing of cognitive resources previously devoted to effortful listening.
Group Experiences and Individual Differences in Stuttering
Authors: Tichenor Seth E. , Yaruss J. Scott 
Published: December 2019

Purpose
This study explored group experiences and individual differences in the behaviors, thoughts, and feelings perceived by adults who stutter. Respondents' goals when speaking and prior participation in self-help/support groups were used to predict individual differences in reported behaviors, thoughts, and feelings.

Method
In this study, 502 adults who stutter completed a survey examining their behaviors, thoughts, and feelings in and around moments of stuttering. Data were analyzed to determine distributions of group and individual experiences.

Results

Speakers reported experiencing a wide range of both overt behaviors (e.g., repetitions) and covert behaviors (e.g., remaining silent, choosing not to speak). Having the goal of
not stuttering when speaking
was significantly associated with more covert behaviors and more negative cognitive and affective states, whereas a history of self-help/support group participation was significantly associated with a decreased probability of these behaviors and states.

Conclusion
Data from this survey suggest that participating in self-help/support groups and having a goal of communicating freely (as opposed to trying not to stutter) are associated with less negative life outcomes due to stuttering. Results further indicate that the behaviors, thoughts, and experiences most commonly reported by speakers may not be those that are most readily observed by listeners.
Mean Length of Utterance and Other Quantitative Measures of Spontaneous Speech in Russian-Speaking Children
Authors: Tomas Ekaterina , Dorofeeva Svetlana 
Published: December 2019

Purpose
This study investigated methodological and theoretical aspects of using mean length of utterance (MLU) and its alternatives in cross-linguistic research and, in particular, its applicability to Russian—a language with a rich system of grammatical and derivational morphemes.

Method
We collected audio recordings of spontaneous speech samples from 27 Russian-speaking children aged between 2;9 and 5;7 (years;months) over individual play sessions. For each participant, we transcribed the first 100 complete utterances and coded them for several types of utterance length measurements, including length in morphemes (grammatical and derivational), words, and syllables. At a sample level, we calculated the average number of produced unique grammatical forms, getting an alternative quantitative estimate of children's morphosyntactic abilities.

Results
A combination of Pearson correlation analysis and Bland–Altman difference plots established that MLU can be reliably used in Russian-speaking children aged around 3;0. The average number of unique grammatical forms remains a sensitive measurement of language capabilities even in older children aged over 3;6. Two quantitative measurements, MLU in syllables and morphemes, show good agreement, suggesting that these measurements can be used interchangeably across studies. Sample size analysis revealed that samples under 75 utterances do not provide sufficient reliability for estimating a child's MLU.

Conclusions
This article demonstrated that MLU can be used in young Russian-speaking children under 3;0–3;6. Also, we showed that the classical morpheme calculation approach can be substituted with counting syllables, which is more time efficient in the absence of automated parsers and is potentially more appropriate for some (e.g., polysynthetic) languages. Our proposed alternative to MLU—the average number of grammatical forms in a sample—appears to be a more sensitive measurement of language capabilities even in older children.
Kinematics of disk galaxies in (proto-)clusters atz= 1.5
Authors: Böhm A. , Ziegler B. L. , Pérez-Martínez J. M. , Kodama T. , Hayashi M. , Maier C. , Verdugo M. , Koyama Y. 
Published: December 2019

Aims.While many aspects of the impact of dense environments on late-type galaxies at redshifts below unity have been scrutinized in the past few decades, observational studies of the interplay between environment and disk galaxy evolution atz >  1 are still scarce. We observed star-forming galaxies atz ≈ 1.5 selected from the HyperSuprimeCam Subaru Strategic Program. The galaxies are part of two significant overdensities of [O II] emitters identified via narrowband imaging and photometric redshifts fromgrizyphotometry.Methods.We used theK-band Multi-Object Spectrograph (KMOS) to carry out Hαintegral field spectroscopy of 46 galaxies in total. Ionized gas maps, star formation rates, and velocity fields were derived from the Hαemission line. We quantified morphological and kinematical asymmetries in order to look for potential gravitational (e.g., galaxy-galaxy) or hydrodynamical (e.g., ram-pressure) interactions.Results.Hαemission was detected in 36 of our targets. Of these galaxies, 34 are members of two (proto-)clusters atz = 1.47, confirming our selection strategy to be highly efficient. By fitting model velocity fields to the observed ones, we determined the intrinsic maximum rotation velocityVmaxof 14 galaxies. Utilizing the luminosity–velocity (Tully–Fisher) relation, we find that these galaxies are more luminous than their local counterparts of similar mass by up to ∼4 mag in the rest-frameB-band. In contrast to field galaxies atz <  1, the offsets of thez ≈ 1.5 (proto-)cluster galaxies from the local Tully–Fisher relation are not correlated with their star formation rates but with the ratio betweenVmaxand gas velocity dispersionσg. This probably reflects that fewer disks have settled to purely rotational kinematics and highVmax/σgratios, as is observed in the field at similar redshifts. Tests with degraded low-redshift cluster galaxy data show that we cannot identify purely hydrodynamical interactions with the imaging currently at hand. Due to relatively low galaxy velocity dispersions (σv <  400 km s−1) of the (proto-)clusters, gravitational interactions are likely more efficient, resulting in higher kinematical asymmetries than in present-days clusters.
λ= 2 mm spectroscopy observations toward the circumnuclear disk of NGC 1068
Authors: Qiu Jianjie , Zhang Jiangshui , Zhang Yong , Jia Lanwei , Tang Xindi 
Published: December 2019

Aims.We investigate the physical and chemical conditions of molecular gas in the circumnuclear disk (CND) region of NGC 1068.Methods.We carried out a spectral line survey with the IRAM 30 m telescope toward the center of NGC 1068 and mainly focused on the 2 mm band with a frequency coverage of 160.7–168.6 GHz and 176.5–184.3 GHz.Results.Fifteen lines are detected in NGC 1068, eight of which are new detections for this galaxy. We derive the rotation temperatures and column densities of fourteen molecular species.Conclusions.Based on the [HCO+(2 − 1)]/[HOC+(2 − 1)] ratio, we obtain a high ionization degree in the CND of NGC 1068. It is found that HC3N is concentrated in the east knot, while13CCH, CH3CN, SO, HOC+, CS, CH3CCH, and H2CO are concentrated in the west knot. Compared to the star-forming galaxies M 82 and NGC 253, the chemistry of NGC 1068 might be less strongly affected by the UV radiation field, and its kinetic temperature might be lower.
The Tarantula Massive Binary Monitoring
Authors: Mahy L. , Sana H. , Abdul-Masih M. , Almeida L. A. , Langer N. , Shenar T. , de Koter A. , de Mink S. E. , de Wit S. , Grin N. J. , Evans C. J. , Moffat A. F. J. , Schneider F. R. N. , Barbá R. , Clark J. S. , Crowther P. , Gräfener G. , Lennon D. J. , Tramper F. , Vink J. S. 
Published: December 2019

Context. Accurate stellar parameters of individual objects in binary systems are essential to constrain the effects of binarity on stellar evolution. These parameters serve as a prerequisite to probing existing and future theoretical evolutionary models.
Aims. We aim to derive the atmospheric parameters of the 31 double-lined spectroscopic binaries in the Tarantula Massive Binary Monitoring sample. This sample, composed of detached, semi-detached and contact systems with at least one of the components classified as an O-type star, is an excellent test-bed to study how binarity can impact our knowledge of the evolution of massive stars.
Methods. In the present paper, 32 epochs of FLAMES/GIRAFFE spectra are analysed by using spectral disentangling to construct the individual spectra of 62 components. We then apply the CMFGEN atmosphere code to determine their stellar parameters and their helium, carbon, and nitrogen surface abundances.
Results. Among the 31 systems that we study in the present paper, we identify between 48 and 77% of them as detached, likely pre-interacting systems, 16% as semi-detached systems, and between 5 and 35% as systems in or close to contact phase. Based on the properties of their components, we show that the effects of tides on chemical mixing are limited. Components on longer-period orbits show higher nitrogen enrichment at their surface than those on shorter-period orbits, in contrast to expectations of rotational or tidal mixing, implying that other mechanisms play a role in this process. For semi-detached systems, components that fill their Roche lobe are mass donors. They exhibit higher nitrogen content at their surface and rotate more slowly than their companions. By accreting new material, their companions spin faster and are likely rejuvenated. Their locations in the N − v sin i diagram tend to show that binary products are good candidates to populate the two groups of stars (slowly rotating, nitrogen-enriched objects and rapidly rotating non-enriched objects) that cannot be reproduced through single-star population synthesis. Finally, we find no peculiar surface abundances for the components in (over-)contact systems, as has been suggested by evolutionary models for tidal mixing.
Conclusions. This sample, consisting of 31 massive binary systems, is the largest sample of binaries composed of at least one O-type star to be studied in such a homogeneous way by applying spectral disentangling and atmosphere modelling. The study of these objects gives us strong observational constraints to test theoretical binary evolutionary tracks.
Extended radio emission associated with a breakout eruption from the back side of the Sun
Authors: Morosan D. E. , Palmerio E. , Lynch B. J. , Kilpua E. K. J. 
Published: December 2019

Context. Coronal mass ejections (CMEs) on the Sun are the largest explosions in the Solar System that can drive powerful plasma shocks. The eruptions, shocks, and other processes associated to CMEs are efficient particle accelerators and the accelerated electrons in particular can produce radio bursts through the plasma emission mechanism.
Aims. Coronal mass ejections and associated radio bursts have been well studied in cases where the CME originates close to the solar limb or within the frontside disc. Here, we study the radio emission associated with a CME eruption on the back side of the Sun on 22 July 2012.
Methods. Using radio imaging from the Nançay Radioheliograph, spectroscopic data from the Nançay Decametric Array, and extreme-ultraviolet observations from the Solar Dynamics Observatory and Solar Terrestrial Relations Observatory spacecraft, we determine the nature of the observed radio emission as well as the location and propagation of the CME.
Results. We show that the observed low-intensity radio emission corresponds to a type II radio burst or a short-duration type IV radio burst associated with a CME eruption due to breakout reconnection on the back side of the Sun, as suggested by the pre-eruptive magnetic field configuration. The radio emission consists of a large, extended structure, initially located ahead of the CME, that corresponds to various electron acceleration locations.
Conclusions. The observations presented here are consistent with the breakout model of CME eruptions. The extended radio emission coincides with the location of the current sheet and quasi-separatrix boundary of the CME flux and the overlying helmet streamer and also with that of a large shock expected to form ahead of the CME in this configuration.
Undetected electron backscattering in Perkeo III
Authors: Roick Christoph , Saul Heiko , Abele Hartmut , Märkisch Bastian 
Published: December 2019

The beta asymmetry in neutron beta decay is used to determine the ratio of axial-vector coupling to vector coupling most precisely. In electron spectroscopy, backscattering of electrons from detectors can be a major source of systematic error. We present the determination of the correction for undetected backscattering for electron detection with the instrument Perkeo III. For the electron asymmetry, undetected backscattering leads to a fractional correction of 5 × 10−4, i.e. a change by 40% of the total systematic uncertainty.
Neutron spin rotation measurements
Authors: Sarsour M. , Amadio J. , Anderson E. , Barrón-Palos L. , Crawford B. , Crawford C. , Esposito D. , Fox W. , Francis I. , Fry J. , Gardiner H. , Haddock C. , Holly A. , Hoogerheide S.F. , Korsak K. , Lieers J. , Magers S. , Maldonado-Velázquez M. , Mayorov D. , Mumm H.P. , Nico J.S. , Okudaira T. , Paudel C. , Santra S. , Shimizu H.M. , Snow W.M. , Sprow A. , Steen K. , Swanson H.E. , Tôvesson F. , Vanderwerp J. , Yergeau P.A. 
Published: December 2019

The neutron spin rotation (NSR) collaboration used parity-violating spin rotation of transversely polarized neutrons transmitted through a 0.5 m liquid helium target to constrain weak coupling constants between nucleons. While consistent with theoretical expectation, the upper limit set by this measurement on the rotation angle is limited by statistical uncertainties. The NSR collaboration is preparing a new measurement to improve this statistically-limited result by about an order of magnitude. In addition to using the new high-flux NG-C beam at the NIST Center for Neutron Research, the apparatus was upgraded to take advantage of the larger-area and more divergent NG-C beam. Significant improvements are also being made to the cryogenic design. Details of these improvements and readiness of the upgraded apparatus are presented. We also comment on how recent theoretical work combining effective field theory techniques with the 1/Nc expansion of QCD along with previous NN weak measurements can be used to make a prediction for dϕ/dz in 4He.
An experiment using the same apparatus with a room-temperature target was carried out at LANSCE to place limits on parity-conserving rotations from possible fifth-force interactions to complement previous studies. We sought this interaction using a slow neutron polarimeter that passed transversely polarized slow neutrons by unpolarized slabs of material arranged so that this interaction would tilt the plane of polarization and develop a component along the neutron momentum. The results of this measurement and its impact on the neutron-matter coupling gA2 from such an interaction are presented. The NSR collaboration is also preparing a new measurement that uses an upgraded version of the room-temperature target to be run on the NG-C beamline; and it is expected to constrain gA2 by at least two additional orders of magnitude for λc between 1 cm and 1 μm.