bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of body posture change on peripherally inserted central catheter tip position in Chinese cancer patients
Authors: Liu Chunli , Jiang Dingbiao , Jin Tao , Chen Chuanyin , Shi Ruchun , Liu Lagen , Mao Jing , Gu Lili , Xu Liyong , Meng Aifeng 
Published: February 2020

Purpose: To evaluate the influence of body posture change on the peripherally inserted central catheter tip position in Chinese cancer patients. Methods: A prospective observational trial was conducted in a tertiary cancer hospital from August to September 2018. After the insertion of peripherally inserted central catheter, chest X-ray films were taken to check the catheter tip in the upright and supine positions, respectively. The distance from the carina to the catheter tip was separately measured on both chest films by nurses. The primary study outcome was the distance and direction of the catheter tip movement. The secondary study outcome was to analyze the influence factors on the catheter tip movement. The third study outcome was to observe the related adverse events caused by the catheter tip movement. Results: A total of 79 patients were included, the results showed that 61 moved cephalad, 14 moved caudally, and 4 did not move with body change from the supine to the upright position. When moved cephalad, the mean distance was 19.34 ± 11.95 mm; when moved caudally, the mean distance was –15.83 ± 8.97 mm. The difference between the two positions was statistically significant ( p < 0.001). There was also a statistically significant difference between catheter tip movement direction and body mass index ( p = 0.009) and height ( p = 0.015). Two patients developed arrhythmias; no cardiac tamponade was found due to body posture change. Conclusion: The results of this work implied that the tips of the catheter tend to shift toward the cephalad with body change from the supine to the upright position. A study involving a larger sample size is needed to find more information in the future.
Optimizing Communication About Topical Corticosteroids: A Quality Improvement Study
Authors: Johnson Girard Valérie , Hill Ashley , Glaser Emma , Lussier Marie-Thérèse 
Published: February 2020

Background Patients are often non-adherent to topical corticosteroids (TCS). This may be in part due to poor communication between patients and dermatologists. Objectives This quality improvement (QI) study aims to describe dermatologist–patient communication about TCS treatments and to compare communication before and after the implementation of an educational intervention. Methods This QI study assesses the communication between dermatologists and new dermatology outpatients receiving a TCS prescription in a tertiary care center. The QI intervention is 2-pronged, consisting of an educational pamphlet for patients and a communication workshop for the dermatology team. Encounters were audiotaped, and communication was analyzed using a coding system (MEDICODE). Phase 1 recordings happened preintervention and reflect the usual dermatologist–patient communication in this practice setting and phase 2 recordings were postintervention. Results Phase 1 reveals that dermatologists frequently address informational medication themes, such as naming the medications and informing patients about their proper use. They less frequently discuss patient experience themes, such as goals of treatment, adverse effects of treatments, and exploring patients’ emotions about medications (such as anxiety, fears, etc.). After the intervention, there was more frequent discussion of patient experience themes without increasing consultation length. But, in both phases, physicians address most themes as a monolog with little verbal input from patients. Conclusions Our study raises awareness regarding dermatologists’ communication patterns about TCS, identifying specific areas for improvement, such as discussions of adverse effects, and explicitly addressing patients’ attitudes and emotions. This is an essential step to foster a sense-making of TCS for patients.
Collagen 2A Type B Induction after 3D Bioprinting Chondrocytes In Situ into Osteoarthritic Chondral Tibial Lesion
Authors: Gatenholm Birgitta , Lindahl Carl , Brittberg Mats , Simonsson Stina 
Published: February 2020

Objective Large cartilage defects and osteoarthritis (OA) cause cartilage loss and remain a therapeutic challenge. Three-dimensional (3D) bioprinting with autologous cells using a computer-aided design (CAD) model generated from 3D imaging has the potential to reconstruct patient-specific features that match an articular joint lesion. Design To scan a human OA tibial plateau with a cartilage defect, retrieved after total knee arthroplasty, following clinical imaging techniques were used: (1) computed tomography (CT), (2) magnetic resonance imaging (MRI), and (3) a 3D scanner. From such a scan, a CAD file was obtained to generate G-code to control 3D bioprinting in situ directly into the tibial plateau lesion. Results Highest resolution was obtained using the 3D scanner (2.77 times more points/mm2 than CT), and of the 3 devices tested, only the 3D scanner was able to detect the actual OA defect area. Human chondrocytes included in 3D bioprinted constructs produced extracellular matrix and formed cartilage tissue fragments after 2 weeks of differentiation and high levels of a mature splice version of collagen type II (Col IIA type B), characteristic of native articular cartilage and aggrecan (ACAN). Chondrocytes had a mean viability of 81% in prints after day 5 of differentiation toward cartilage and similar viability was detected in control 3D pellet differentiation of chondrocytes (mean viability 72%). Conclusion Articular cartilage can be formed in 3D bioprints. Thus, this 3D bioprinting system with chondrocytes simulating a patient-specific 3D model provides an attractive strategy for future treatments of cartilage defects or early OA.
Increasing Concentration of Sinovial: Effect on Cartilage Protection in a Rabbit ACLT Model
Authors: Boissier Pomme , Mainil-Varlet Pierre , Mautone Giuseppe R. 
Published: February 2020

Objective This study aimed to test the hypothesis that administration of increasing doses of Sinovial (hyaluronic acid [HA]), would exhibit a dose-dependent effect on the prevention of cartilage degradation, without local and systemic toxicity. Methods Twenty-seven adult rabbits were subjected to anterior cruciate ligament transection (ACLT). Two Sinovial products containing HA concentrations of 1.6% and 2.4% were used as active treatment, and 0.9% saline was used as control and injected intra-articularly 7 days post ACLT. Radiographs were taken prior to surgery, at injection and sacrifice times. After euthanasia, 8 weeks postsurgery, knee joints were observed macroscopically using India ink staining with OARSI (Osteoarthritis Research Society International) scoring and histologically using modified Mankin scoring. The synovial membranes were analyzed using Cake classification. Results No intraoperative complications were observed. One week postinjection, 4 animals in the HA 2.4% group developed subcutaneous nodules that disappeared spontaneously. No inflammation of the synovial membrane was ever observed. The control group exhibited the maximum uptake of India ink 2.22 ± 0.14. HA groups exhibited a dose-dependent ( P = 0.02) reduction in India ink uptake: 1.75 ± 0.17 for HA 1.6% and 1.58 ± 0.14 for HA 2.4%. The most marked dose-dependent effect of this study was a reduction of modified Mankin score for HA groups, with the 2.4% treatment achieving a statistically significant improvement as compared with the control group (7.19 ± 0.85 for saline, 4.65 ± 0.66 for HA 1.6%, and 3.53 ± 0.59 for HA 2.4%; P = 0.005). Conclusions A dose-dependent protective effect on cartilage was observed after injection of both HA solutions.
Corporate Governance Deviance: A Case Study of Infosys
Authors: Chatterjee Anindita , Hazarika Deepti D. 
Published: February 2020

The major organizational transformation journey of Indian software bellwether firm, Infosys, from 2014 until 2017 under the leadership of first non-founder member is captured in this case study. The entrepreneurial ambitions and zeal to grow through organic and inorganic moves saddled with lack of cognizance of corporate governance resulted in deviance leading to turmoil followed by the resignation of the CEO. The company values, the roles of founder members, institutional investors, shareholders and other aspects came under the scanner to understand the reasons behind the corporate governance deviance. Dilemma Though the company got a clean chit against the corporate governance deviance charges, the conflict between the board of governors and founders, particularly the ex-CEO continued which culminated into the resignation of the CEO. What are the roles of founder members, board of governors, institutional investors and shareholders in case of corporate governance deviance? Theory: Models of Corporate Governance Type of the case: Decisional, applied Protagonist: CEO of the firm Options After obtaining clean chit against the corporate governance deviance charges, the CEO could have continued. Instead he decided to resign. Discussions and Case Questions Why the chairman of the board was indecisive in questioning the CEO undertaking deviant business practices? Why were the founders not happy with the ‘non-transparent’ way in which the board had decided to raise Dr Sikka’s annual compensation package from US$7.08 million to US$11 million even when it was linked with the company’s progress towards ‘Vision 2020’? What kind of risk did the founders saw in the appointment of the wife a sitting minister in the Indian government as an independent director? Why the board decided to give a generous severance package to ex-CFO without the approval of the chairperson of Nominations and Remuneration Committee?
Primary or secondary wound healing of the pin sites after removal of the external fixator: study protocol for a prospective, randomized controlled, monocenter trial
Authors: Roth Franz , Cagienard Flavio , Link Björn C. , Hodel Sandro , Lehnick Dirk , Babst Reto , Beeres Frank J. P. 
Published: February 2020

Abstract
Background
Temporary fixation with an external fixator is used for numerous indications in orthopedic trauma surgery. It is unclear whether primary wound healing or secondary open-wound healing after removal of the external fixator should be advocated for the pin site. This study compares primary wound closure with secondary wound healing for the pin site. The primary aim is to compare pin-site infection rates. The secondary aim is to compare time to wound healing and esthetic outcome. The hypothesis was that primary wound closure does not lead to more infections than secondary wound healing.

Methods and design
This is a prospective, randomized controlled, blinded, monocenter study based on a non-inferiority design. To obtain an equal patient population and groups, all pin-entry sites of the patients are treated alternately at the time of removal of the external fixator with primary wound closure and secondary wound healing. Patients are randomized according to whether the proximal pin-entry site is treated with wound closure or by secondary open-wound healing, from which the further sequence develops. The pre- and postoperative protocol is standardized for all pin-entry sites. A photo documentation of the pin-entry sites takes place 2 and 52 weeks postoperatively during the routine clinical follow-up visits. Further controls take place at 6, 12 and 26 weeks after pin removal.
The primary outcome was to demonstrate the non-inferiority of primary wound closure compared to secondary wound healing in terms of postoperative wound infections according to the Center of Disease Control and Prevention (CDC) definitions.
The secondary outcomes are time to complete wound healing (days) and esthetical outcome (subjective preference of patients and Vancouver Scar Scale score).

Discussion
This study aims to answer how to deal with the pin site after removal of the external fixator. To date, no routine and generally accepted protocol exists for the management of pin sites after removal of the external fixator. This prospective, randomized controlled, blinded monocenter trial should determine whether primary wound closure or secondary wound healing should be advocated after removal of the external fixator.

Trial registration
ClinicalTrials.gov, ID: NCT03842956. Registered retrospectively on 13 February 2019.
Yellow fever epizootics in non-human primates, Southeast and Northeast Brazil (2017 and 2018)
Authors: Mares-Guia Maria Angélica Monteiro de Mello , Horta Marco A. , Romano Alessandro , Rodrigues Cíntia D. S. , Mendonça Marcos C. L. , dos Santos Carolina C. , Torres Maria C. , Araujo Eliane S. M. , Fabri Allison , de Souza Everton R. , Ribeiro Roberta O. R. , Lucena Fabiana P. , Junior Luiz C. A. , da Cunha Rivaldo V. , Nogueira Rita M. R. , Sequeira Patricia C. , de Filippis Ana M. Bispo 
Published: February 2020

Abstract
Background
Yellow fever (YF) is a severe, infectious, but non-communicable arboviral hemorrhagic disease. In the last decades, yellow fever virus (YFV) infections have been prevalent in endemic areas in Brazil, affecting human and non-human primate (NHP) populations. Monitoring of NHP infection started in 1999, and reports of epizootic diseases are considered important indicators of viral transmission, particularly in relation to the sylvatic cycle. This study presents the monitoring of YFV by real-time RT-PCR and the epidemiological findings related to the deaths of NHPs in the south-eastern states and in the north-eastern state of Bahia, during the outbreak of YF in Brazil during 2017 and 2018.

Methods
A total of 4198 samples from 2099 NHPs from south-eastern and north-eastern Brazilian states were analyzed by real-time reverse transcription polymerase chain reaction (rtRT-PCR).

Results
A total of 4198 samples from 2099 NHPs from south-eastern and north-eastern Brazilian states were collected between 2017 and 2018. The samples were subjected to molecular diagnostics for YFV detection using real-time reverse transcription polymerase chain reaction (rtRT-PCR) techniques. Epizootics were coincident with human YF cases. Furthermore, our results showed that the YF frequency was higher among marmosets (Callithrix sp.) than in previous reports. Viremia in species of the genus Alouatta and Callithrix differed greatly.

Discussion
Our results indicate a need for further investigation of the role of Callithrix spp. in the transmission cycles of YFV in Brazil. In particular, YFV transmission was observed in a region where viral circulation has not been recorded for decades and thus vaccination has not been previously recommended.

Conclusions
This highlights the need to straighten epizootic surveillance and evaluate the extent of vaccination programmes in Brazil in previously considered “YFV-free” areas of the country.
A chromosomal connectome for psychiatric and metabolic risk variants in adult dopaminergic neurons
Authors: Espeso-Gil Sergio , Halene Tobias , Bendl Jaroslav , Kassim Bibi , Ben Hutta Gabriella , Iskhakova Marina , Shokrian Neda , Auluck Pavan , Javidfar Behnam , Rajarajan Prashanth , Chandrasekaran Sandhya , Peter Cyril J. , Cote Alanna , Birnbaum Rebecca , Liao Will , Borrman Tyler , Wiseman Jennifer , Bell Aaron , Bannon Michael J. , Roussos Panagiotis , Crary John F. , Weng Zhiping , Marenco Stefano , Lipska Barbara , Tsankova Nadejda M. , Huckins Laura , Jiang Yan , Akbarian Schahram 
Published: February 2020

Abstract
Background
Midbrain dopaminergic neurons (MDN) represent 0.0005% of the brain’s neuronal population and mediate cognition, food intake, and metabolism. MDN are also posited to underlay the neurobiological dysfunction of schizophrenia (SCZ), a severe neuropsychiatric disorder that is characterized by psychosis as well as multifactorial medical co-morbidities, including metabolic disease, contributing to markedly increased morbidity and mortality. Paradoxically, however, the genetic risk sequences of psychosis and traits associated with metabolic disease, such as body mass, show very limited overlap.

Methods
We investigated the genomic interaction of SCZ with medical conditions and traits, including body mass index (BMI), by exploring the MDN’s “spatial genome,” including chromosomal contact landscapes as a critical layer of cell type-specific epigenomic regulation. Low-input Hi-C protocols were applied to 5–10 × 103 dopaminergic and other cell-specific nuclei collected by fluorescence-activated nuclei sorting from the adult human midbrain.

Results
The Hi-C-reconstructed MDN spatial genome revealed 11 “Euclidean hot spots” of clustered chromatin domains harboring risk sequences for SCZ and elevated BMI. Inter- and intra-chromosomal contacts interconnecting SCZ and BMI risk sequences showed massive enrichment for brain-specific expression quantitative trait loci (eQTL), with gene ontologies, regulatory motifs and proteomic interactions related to adipogenesis and lipid regulation, dopaminergic neurogenesis and neuronal connectivity, and reward- and addiction-related pathways.

Conclusions
We uncovered shared nuclear topographies of cognitive and metabolic risk variants. More broadly, our PsychENCODE sponsored Hi-C study offers a novel genomic approach for the study of psychiatric and medical co-morbidities constrained by limited overlap of their respective genetic risk architectures on the linear genome.
The status of Clibanarius erythropus after a recent range expansion to Great Britain, with the highest latitude recording of a gravid individual
Authors: Patterson Christophe , Slater Matt , Early Regan , Laing Chris 
Published: February 2020

Abstract
Background
In 2016, the range of the hermit crab Clibanarius erythropus expanded to South West Britain for the second time. C. erythropus primarily lives in the Mediterranean and the Atlantic coast of Europe from the Bay of Biscay to Morocco. The species has now been recorded on both the north and south coast of the South West peninsula of the UK from Newtrain Bay, on the north coast of Cornwall, to Wembury, on the south coast of Devon. It is unknown if the crab’s reappearance in the UK has been caused by a one-off colonisation event or by a continued influx of larvae.

Results
The population in the UK is made up of individual within a narrow size bracket, indicating a single colonisation event took place, and that the population is an ageing one. However, we also report the highest latitude recording of a gravid individual for the species.

Conclusion
A lack of gravid individuals was suggested to be why the species was unable to sustain its presence in the UK following a previous colonisation in 1960. This discovery hints that rising water temperatures may allow C. erythropus and other warm-water species to expand and sustain themselves in the UK. We also found crossover in shell utilisation between C. erythropus and the native hermit crab Pagurus bernhardus, suggesting that competition might occur between the two species.
Nutritional value and impact of wheatgrass juice (Green Blood Therapy) on increasing fertility in male albino rats
Authors: Eissa Hesham A. , Mohamed Sherif S. , Hussein Ahmed M. S. 
Published: February 2020

Abstract
Background and objective
The wheatgrass juice (WGJ) contains a high concentration of vitamin C. WGJ contains a lot of highly functional nutritive ingredients potent to unify the liver with the kidneys for detoxification of the organs and filtration of the blood to build a strong immune system. Also, it boosts fertility and increases sexual desire because of the high magnesium content in phytochemical pigment (chlorophyll) which boosts the production of the enzymes that restores sex steroids.

Materials and methods
The experimental animals were divided into 3 groups of 8 rats. The first group (G1) was fed on the standard normal diet. The same feeding was used also in the second group (G2) and third group (G3). But in the G2, a pharmaceutical formula (contain zinc and vitamin A) was used as a food supplement to increase fertility, and in the G3, wheatgrass juice of 11 mg/day was applied. Hunter L, a, and b values; glucose, fructose, and sucrose contents; pH; total soluble solids (TSS); acidity; concentration of vitamin C and vitamin B complex contents; and phenolic compounds of wheatgrass quality juice were measured.

Results
The juice samples showed L* value of 21.78 (as a lightness index), a* value of − 7.11 (as a redness index), b* values of 17.35 (as a yellowness index), pH (6.7), TSS (5°Bx), and acidity (0.00992%). In the same time, wheatgrass chlorophyll represents 70% of its total chemical constituents that is an antioxidant and rebuilds the bloodstream. In addition, it gives the juice its distinctive green color which is the major quality factor in juice products. The results amounted to normal values of vital organs such as the liver and kidney functions in all groups. The values of aspartate aminotransferase (AST) were 27.88 ± 2.10, 22.50 ± 4.93, and 23.25 ± 4.71 μ/ml in G1, G2, and G3, respectively. Meanwhile, also the results of sexual hormones indicated an elevation in testosterone hormone in G3 (2.90 ± 0.26 ng/ml) than the normal negative control (2.78 ± 0.23 ng/ml) and pharmaceutical formula positive control (2.04 ± 0.40 ng/ml). However, follicle-stimulating hormone (FSH) decreased to 1.44 ± 0.28 IU/L and 1.45 ± 0.24 for G3 and G2, respectively, compared to 1.65 ± 0.23 IU/L in G1.

Conclusion
The findings proved that WGJ increased fertility and promoted youthfulness, and the wheatgrass (WG) has the potential to be used as a “functional herb” containing natural bioactive compounds.
Atezolizumab (atezo) therapy for upper tract (UT) urothelial carcinoma (UC): Subgroup analysis of the single-arm international SAUL study in pretreated locally advanced/metastatic urinary tract carcinoma.
Authors: Sternberg Cora N. , Loriot Yohann , Tambaro Rosa , Buttigliero Consuelo , Morelli Franco , Papai Zsuzsanna , Retz Margitta , Necchi Andrea , Van Der Heijden Michiel Simon , Faust Guy , Cardona Zorrilla Andres Felipe , Lainez Nuria , Panni Stefano , Etxaniz Olatz , Krieger Laurence , Mendez Vidal Maria Jose José , Los Maartje , de Ducla Sabine , Fear Simon , Merseburger Axel Stuart 
Published: February 2020

488 Background: UTUC is rarer than bladder UC and typically responds poorly to standard chemotherapy. Analysis of 220 biomarker-evaluable atezo-treated patients (pts) in phase II/III trials suggested worse outcomes in UT vs lower tract UC [Galsky, ESMO 2018]. We explored clinical outcomes in pts with UTUC (renal pelvis or ureter) treated with atezo in the SAUL study. Methods: The single-arm SAUL study (NCT02928406) [Sternberg, Eur Urol 2019] enrolled a broader pt population, including pts with poor clinical characteristics and/or immune-mediated conditions, more representative of real-world practice than typically enrolled in randomized phase III immunotherapy trials. Pts with urinary tract carcinoma received atezo 1200 mg q3w until disease progression/unacceptable toxicity. Baseline characteristics, safety and efficacy were analyzed in subgroups of pts with UTUC (subdivided into renal pelvis or ureter UC) vs bladder UC. Results: Baseline characteristics in the 4 subgroups were generally similar, except for a numerically lower proportion of pts with 0 prior lines of therapy for metastatic disease in the UTUC vs bladder UC subgroup (30% vs 41%). Treatment exposure, safety and efficacy are shown below. Conclusions: These exploratory analyses of SAUL showed very similar efficacy and safety in UT vs bladder UC. This provides reassurance that atezo is active and has an acceptable safety profile in pts with UTUC, who are generally expected to have worse outcomes than bladder UC pts. Clinical trial information: NCT02928406 . [Table: see text]