bluuebunny/crossref_metadata_2025_split huggingface dataset:
X-ray Spectroscopy Meets Native Mass Spectrometry: Probing Gas-phase Protein Complexes
Authors: Kung Jocky C. K. , Kádek Alan , Kölbel Knut , Bandelow Steffi , Bari Sadia , Buck Jens , Caleman Carl , Commandeur Jan , Damjanović Tomislav , Dörner Simon , Fahmy Karim , Flacht Lara , Heidemann Johannes , Huynh Khon , Kopicki Janine-Denise , Krichel Boris , Lockhauserbäumer Julia , Lorenzen Kristina , Lu Yinfei , Pogan Ronja , Rehmann Jasmin , Schamoni-Kast Kira , Schwob Lucas , Schweikhard Lutz , Springer Sebastian , Svensson Pamela H.W. , Simke Florian , Trinter Florian , Toleikis Sven , Kierspel Thomas , Uetrecht Charlotte 
Published: February 2025

AbstractGas-phase activation and dissociation studies of biomolecules, proteins and their non-covalent complexes using X-rays hold great promise for revealing new insights into the structure and function of biological samples. This is due to the unique properties of X-ray molecular interactions, such as site-specific and rapid ionization. In this perspective, we report and discuss the promise of first proof-of-principle studies of X-ray-induced dissociation of native biological samples ranging from small 17 kDa monomeric proteins up to large 808 kDa non-covalent protein assemblies conducted at a synchrotron (PETRA III) and a free-electron laser (FLASH2). A commercially available quadrupole time-of-flight mass spectrometer (Q-ToF2, Micromass/Waters), modified for high-mass analysis by MS Vision, was further adapted for integration with the open ports at the corresponding beamlines. The protein complexes were transferred natively into the gas phase via nano-electrospray ionization and subsequently probed by extreme ultraviolet (FLASH2) or soft X-ray (PETRA III) radiation, in either their folded state or following collision-induced activation in the gas phase. Depending on the size of the biomolecule and the activation method, protein fragmentation, dissociation, or enhanced ionization were observed. Additionally, an extension of the setup by ion mobility is described, which can serve as a powerful tool for structural separation of biomolecules prior to X-ray probing. The first experimental results are discussed in the broader context of current and upcoming X-ray sources, highlighting their potential for advancing structural biology in the future.
A Novel Method for Culturing Telencephalic Neurons in Axolotls
Authors: Bostan Sevginur , Serdengeçtİ Safiye , Bayat F. Kemal , Bay Sadık , Sezer Ayşe Server , Ayşİt Neşe , Öztürk Gürkan 
Published: February 2025

AbstractThe axolotl (Ambystoma mexicanum), a neotenic salamander with remarkable regenerative capabilities, serves as a key model for studying nervous system regeneration. Despite its potential, the cellular and molecular mechanisms underlying this regenerative capacity remain poorly understood, partly due to the lack of reliablein vitromodels for axolotl neural cells. In this study, we developed a novel protocol for primary cultures of adult axolotl telencephalon/pallium, enabling the maintenance of viable and functionally active neural cells. Using calcium imaging and immunocytochemistry, we demonstrated the presence of neuronal and glial markers, synaptic connections, and spontaneous calcium activity, highlighting the functional integrity of the cultured cells. Our findings reveal that these cultures can be maintained in both serum and serum-free conditions, with neurons exhibiting robust neurite outgrowth and responsiveness to injury. This protocol addresses a critical gap in axolotl research by providing a controlledin vitrosystem to study neurogenesis and regeneration. By offering insights into the regenerative mechanisms of axolotl neurons, this work lays the foundation for comparative studies with mammalian systems, potentially informing therapeutic strategies for neurodegenerative diseases and CNS injuries in humans.
The Impact of Negative Emotions on Adolescents’ Nonsuicidal Self-Injury Thoughts: An Integrated Application of Machine Learning and Multilevel Logistic Models
Authors: Ahn Chan-Young , Kim Jin-Ha , Kim So-Jung , Kim Jae-Won , Na Jung-Jo , Seo Dong-Gi , Lee Jong-Sun 
Published: February 2025

AbstractNon-Suicidal Self-Injury (NSSI) is a prevalent and complex behavior among adolescents, often linked to negative emotions such as loneliness, anxiety, and emptiness. Traditional retrospective methodologies often fail to capture the dynamic and real-time nature of NSSI and its emotional triggers due to memory biases and temporal fluctuations. This study aimed to identify emotional predictors of NSSI thoughts among adolescents using machine learning and multilevel logistic regression.The study included 42 adolescents (aged 12–15 years) who had engaged in NSSI in the past year. Participants reported their mood and NSSI behaviors three times daily over a 14-day EMA period via a smartphone application. Predictor variables included depression, anxiety, loneliness, self-anger, anger towards others, shame, and emptiness. A random forest model identified loneliness (feature importance: 0.40), anxiety (0.18), and emptiness (0.14) as the most significant predictors of NSSI thoughts. Multilevel logistic regression confirmed these findings, showing that each one-unit increase in anxiety, loneliness, and emptiness corresponded to a 24%, 19%, and 24% increase in the odds of experiencing NSSI thoughts, respectively. The ICC value of 0.26 indicated substantial between-individual variance, justifying multilevel modeling. However, random effects analysis revealed no significant individual differences, suggesting uniform effects across participants.These findings highlight loneliness as the most influential predictor, emphasizing the need to address social connections in interventions. Combining machine learning with traditional statistical methods enhanced interpretability, providing practical insights for developing tailored, emotion-focused interventions for adolescents engaging in NSSI.
Activity of Carbazole, Aminoguanidine and Diamine Anti-infectives againstToxoplasma gondii
Authors: Singh Davinder , Sleda Melissa A. , Pandey Akanksha , Malwal Satish R. , Chen Yiyuan , Zhou Ruijie , Adewole Feyisola , Sadowska Katie , Onajole Oluseye K. , Moreno Silvia N.J. , Oldfield Eric 
Published: February 2025

ABSTRACTWe report the observation that carbazole anti-infectives developed as antibacterial and antifungal drug leads have activity against the tachyzoite-stage growth of the Apicomplexan parasiteToxoplasma gondiiwith IC50values as low as 2 μM. We show that a phenylthiazole aminoguanidine with antibacterial as well as antifungal activity inhibits growth with an IC50value of 2.1 μM. We also tested a series of 18 analogs of the diamine SQ109, a tuberculosis drug candidate which likewise has both antibacterial and antifungal activity, finding activity as low as 2.3 μM. We tested all compounds for their activity in collapsing the ΔpH component of the promotive force, the results indicating that all compounds acted, at least in part, as protonophore uncouplers. Finally, we also investigated the correlation between the activity of all compounds against the yeastSaccharomyces cerevisiaeand the bacteriumMycobacterium smegmatis, finding significant correlations between the collapse of the proton motive force and anti- fungal/antibacterial activity.
“It falls upon you to create a better place”: exploring ephemeral online communities under the lens of organisational configurations
Authors: Jemine Grégory 
Published: February 2025

Purpose
This study aims to extend Mintzberg’s theory of organisational configurations to the specific context of ephemeral online communities. As such communities self-organise rapidly and temporarily, without fixed hierarchies, authority relationships or formal structures, they represent a novel and challenging subject of study from an organisational perspective. In this paper, the author argues that configuration theory can nevertheless be a powerful framework to understand how such communities organise collective action.

Design/methodology/approach
An analysis of a social experiment organised by the Reddit platform called “The Place” is used to study emergent coordination mechanisms in ephemeral online communities. Drawing on data gathered from Reddit and from the experiment’s outcomes, this paper focuses upon the coordinating mechanisms deployed by different communities to establish themselves in a position of power.

Findings
Using configuration theory, the author outlines four main ways for the participants to coordinate their actions in ephemeral online contexts. The findings suggest that, beyond the study of more traditional organisational contexts, configuration theory is a powerful framework for understanding more emergent and contemporary forms of organising.

Originality/value
This paper assesses the validity of a well-rounded theory in organisation studies, configuration theory, to study a novel type of organisations – ephemeral online communities. In doing so, it offers new insights into how online environments can facilitate and structure collective action without traditional organisational structures, thus advancing the study of online coordination mechanisms.
Social enterprise development in Ireland: making a case for actor-network-theory
Authors: Quinn Úna , McCusker Paul , Gallagher Padraig 
Published: February 2025

PurposeThe development of social enterprises in Ireland holds much promise with regards to the potential to create innovation and growth within local economies. However, fulfilling this promise requires the ability to recognise the complexities of this multifaced sector. In response, this article proposes Actor-Network-Theory (ANT) as having the theoretical capability to develop and create further progress in social enterprise development in Ireland.Design/methodology/approachANT upholds that reality consists of series of heterogeneous networks composed of relations between entities. This relational lens foregrounds the interactions among the varied actors involved in social enterprise. To investigate the capabilities of ANT, this paper aligns ANT with three core areas of concern for social enterprise research: structure, power and shared meaning.FindingsANT works to address these identified key concerns by bridging the conceptual divide between theory and practice within the social enterprise sector. This brings to light the state of affairs as empirically practiced and not as recognised under traditional dualisms. As a result, the influences which shape the collective reality of social enterprise networks can be empirically investigated.Originality/valueAs an alternative sensibility, the core capabilities of ANT lie in the ability of the theory to reclaim an empirical grasp of a local environment. In this instance, it is demonstrated as to how this theoretical approach can be used to understand how growth and development manifests within social enterprise networks. From this, there is a great potential to use these insights to direct change in the place of random, and often contested, growth.
Mapping global trends and future opportunities in Islamic banking windows: a 15-year bibliometric perspective (2008–2023)
Authors: Mainata Dedy , Hanafi Mamduh M.M. , Setiyono Bowo 
Published: February 2025

Purpose
Islamic banking windows have attracted a considerable deal of scholarly attention lately, as shown by the sharp rise in publications across the globe. The growing number of Islamic banking windows being published demonstrates how much scholarly interest there has been in this topic. To evaluate the body of knowledge on Islamic banking windows, this paper aims to conduct a bibliometric analysis.

Design/methodology/approach
A bibliometric examination of literature samples pulled in July 2023 from the Scopus database served as the study’s methodology. In total, 79 publications that met the criteria for selection and were published between 2008 and 2023 made up the study’s sample size. Software like RStudio (Rshiny), VOSviewer and Microsoft Excel have been used to evaluate the data. Three major kinds of analyses were carried out: overall performance indicators, citation analysis and cross-dimensional keyword analysis. A quick content analysis to pinpoint research flows then followed.

Findings
The number of publications increased noticeably between 2019 and 2021, with Abdul Majid M. contributing the most with four articles published during that time. The findings also include the countries, groups, publications, works, sources and topics that have contributed the most. This examination found five research streams that have already been examined by other writers out of 79 papers. Comparative efficiency, Islamic banking’s customer, performance, risk and liquidity management are some of the research areas covered. With precise research questions, this study further outlined the future research agenda.

Research limitations/implications
The scope of this study is restricted to English-language journal articles appearing in sources that are Scopus-indexed. A variety of databases, including Dimensions and the Web of Science, as well as expanding the investigated units, could be used in future research. Future studies might center on the systematic review of publications published on certain topics, whereas this study only focused on bibliometric analysis and research streams.

Originality/value
Despite the fact that Islamic banking is a fascinating topic for research, as far as the authors’ knowledge, this study is the first bibliometric analysis of Islamic banking windows. The five research streams identified in this study also offer numerous potential research topics in a unique way.
Trickle-down effects of supervisors’ family-to-work enrichment and conflict: the roles of supervisor support and dyadic similarity
Authors: Wang Peng , Steed Laurens Bujold , Wang Zhen 
Published: February 2025

PurposeDrawing upon the Conservation of Resources theory and Relational Demography Theory, we examine the crossover of supervisor family experiences to subordinates in the workplace. We posit that supervisor family-to-work enrichment and conflict influence subordinate perceptions of supervisor support for work–family, which, in turn, positively affects subordinate work engagement and job satisfaction and negatively affects subordinate turnover intentions. The effects of supervisor family-to-work enrichment and conflict on perceptions of supervisor support are respectively suggested to be strengthened and weakened by the demographic similarity between the supervisor and the subordinate.Design/methodology/approachUsing a sample of 496 employees nested within 83 supervisors from China, we conducted a multilevel analysis.FindingsOur results indicate that supervisor family-to-work conflict and supervisor family-to-work enrichment have negative and positive effects (respectively) on subordinates’ perceptions of supervisor work–family support, and this effect is moderated by low, rather than high, similarity in the supervisor–subordinate dyad. An overall indirect effect of supervisor family-to-work enrichment and family-to-work conflict on subordinate work engagement, job satisfaction and turnover intentions through the mediator of perceived supervisor work–family support is also confirmed.Practical implicationsFrom a practical standpoint, our research emphasizes the importance for organizations to support supervisors in achieving work–family balance in order to promote positive employee work-related outcomes.Originality/valueOur study contributes to work–family literature by unraveling how and when resources may travel through supervisors to affect the generation of new resources (i.e. supervisor support for work–family) and ultimately affect subordinate outcomes in the workplace.
Analysis of the Functional Role of TIMM29 in the Hepatitis B Virus Life Cycle
Authors: Abueldahab Limia , Suwanmanee Yadarat , Muriungi Nelly , Ohsakai Eriko , Wada Masami , Kimura‐Ohba Shihoko , Ueda Keiji 
Published: February 2025

ABSTRACTHepatitis B virus (HBV) causes chronic hepatitis B, which can progress to liver cirrhosis and hepatocellular carcinoma. HBV has complex interactions with various cell organelles and proteins that ensure effective progeny virus production. We previously reported that a mitochondrial protein, TIMM29, should regulate the HBV life cycle through interactions with the HBV preS1 protein. Here, we established Halo‐TIMM29wt‐, Halo‐TIMM29:∆99–192‐, and Halo‐TIMM29:92–194‐expressing cells using TIMM29‐knockout HB611 (TIMM29KO/HB611) cells, a stably HBV‐producing cell line based on Huh6 cells. We found that HBV antigen expression and replication were downregulated in cells stably expressing full‐length TIMM29, but not in those expressing TIMM29 deletion mutants. On the other hand, in the case of TIMM29‐knockout C4 (TIMM29KO/C4), which is a human NTCP‐expressing HepG2 cell line that is competent for HBV infection and amplification, these phenomena were not reproduced, except in full‐length TIMM29 (Halo‐TIMM29wt)‐expressing cells. Using gene expression microarrays, we identified downregulation of ARRDC3 and BASP1 in TIMM29KO/HB611 and TIMM29KO/C4. It was suggested that TIMM29 localized at the mitochondrial inner membrane served as a signaling hub, orchestrating the activation of ARRDC3 and BASP1 expression to restrict HBV transcription. The expression of TIMM29 mutants in TIMM29KO/HB611 and TIMM29KO/C4 cells suggested that ARRDC3 was dependent on the HBV preS1‐binding region of TIMM29 (amino acids 99–189). In contrast, BASP1 expression varied according to cell type, indicating additional regulatory mechanisms. Thus, this study should significantly advance our understanding of TIMM29‐mediated inhibition of HBV amplification and lead to improvements in antiviral strategies and therapeutic interventions against HBV.
Grazing regulates temperate grassland multidimensional stability facing extreme winter snowfall reductions by influencing below‐ground bud density
Authors: Guo Tongtian , Zhang Hao , Guo Meiqi , Yang Gaowen , Xu Hengkang , Jing Xin , Liu Nan , Zhang Yingjun 
Published: February 2025

Abstract

Global climate changes increased the frequency of snowfall reduction events in the Northern Hemisphere, consequently suppressing plant productivity. Grazing, the most widespread use of grasslands, influences productivity in response to climatic extremes by shaping community structure. Since grazing could disrupt normal plant growth and reproduction, rest from grazing at various stages of the growing season may have different effects on above‐ and below‐ground community properties. However, how grazing or grazing rest at different stages of growing season affects grassland stability when facing extreme snowfall reduction remains unclear.

We investigated the multidimensional stability (resistance, resilience, recovery and temporal stability) of above‐ground net primary productivity (ANPP) under a time‐dependent strategic‐rest grazing practice (rest in early, peak and late growing stage) experiment, during which a natural extreme snowfall reduction event occurred. We also assessed plant richness, dominance, asynchrony, key functional group stability and below‐ground bud density to explore the mechanisms underlying multidimensional stability.

We found that extreme snowfall reduction significantly decreased grassland ANPP under all grazing practices. However, grazing with short‐term rest during the peak growing stage significantly enhanced ANPP, improved resistance and recovery from extreme snowfall reduction, and consequently greatly improved the temporal stability compared to continuous grazing. In contrast, the grazing rest during the early and late growing stages did not improve temporal stability of ANPP. Meanwhile, resilience was not affected by grazing practices. The benefits of peak rest primarily arise from allowing the formation of sufficient below‐ground buds before and after extreme events. Notably, changes in community properties (such as diversity or asynchrony) resulting from the rest were not correlated with resistance and recovery. Additionally, the increases in grass bud density from the peak rest indirectly contributed to temporal stability by enhancing the stability of perennial rhizome grass and preserving community composition.

Synthesis. These findings underscore the essential role of plant below‐ground buds in sustaining stable grassland productivity in response to snowfall reduction and also suggest that grassland management strategies should account for the protection of plant asexual reproductive organs, which contributes to grassland sustainability in the face of future climate change.
Hair root sonication washing impact on nuclei counts
Authors: Betts Stephanie M. , Espinoza Quiroga Ivette A. , Otterstatter Linda M. , Donfack Joseph 
Published: February 2025

AbstractIn a previous study, hair samples were washed by sonication in Terg‐a‐zyme™ (Alconox, White Plaines, NY, USA) as part of the DNA extraction protocol. The sonication wash step was deemed necessary in the previous study because the DNA extracts could be used for both mitochondrial (mt) and nuclear(nu) DNA analyses. In the current study, the impact of the sonication wash on the persistence of nuclei was assessed. Scalp hair roots were stained using the DNA binding dye 4′, 6‐diamidino‐2‐phenylindole (DAPI) and visible nuclei were counted. Hair roots that contained <50 visible nuclei were primarily used to monitor the change in the number of visible nuclei due to sonication. Hair roots with a larger number of nuclei counts (e.g., >100) were also included as comparative data. The selected hair samples were washed by sonication in Terg‐a‐zyme™. The number of nuclei detected in unwashed DAPI‐stained hair roots was compared with the number in washed DAPI‐stained hair roots. Intricacies that impeded visible nuclei counting in the same hair root were observed, such as orientation of the mounted hair root, hair root morphology, turgescence, and folding of soft tissue. Despite these challenges, this study showed the sonication wash of hair roots containing <50 visible nuclei might lead to a reduction of nuclei available for DNA extraction and short tandem repeat (STR) typing. However, for laboratories performing only nuDNA analysis, the sonication could be replaced with a less aggressive hair washing method such as briefly vortexing at low speed in saline followed by an ethanol rinse.