bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of rhein on bile acid homeostasis in rats
Authors: Xian Zhong , Tian Jingzhuo , Wang Lianmei , Zhang Yushi , Han Jiayin , Deng Nuo , Liu Suyan , Zhao Yong , Li Chunying , Yi Yan , Wang Dunfang , Meng Jing , Pan Chen , Liang Aihua 
Published: January 2020

AbstractRhein, the active ingredient of rhubarb, a medicinal and edible plant, is widely used in clinical practice. In this work, we investigated the alterations of 14 bile acids and hepatic transporters after rats were administered rhein for 5 consecutive weeks. There was no obvious injury to the liver and kidney, and there were no significant changes in biochemical indicators. However, 1,000 mg/kg rhein increased the liver total bile acid (TBA) levels, especially taurine-conjugated bile acids (t-CBAs), inhibited the expression of Farnesoid X receptor (FXR) and (bile salt export pump) BSEP mRNA, and upregulated the expression of (cholesterol 7α-hydroxylase) CYP7A1 mRNA. Rhein close to the clinical dose reduced the amounts of TBAs, especially unconjugated bile acids (UCBAs), and elevated the expression of FXR and multidrug resistance-associated protein 3 (Mrp3) mRNA. These results denote that rhein is not toxic and is safe to use at a reasonable dose and timing.
Systematic Normalization with Multiple Housekeeping Genes for the Discovery of Genetic Dependencies in Cancer
Authors: Bonham-Carter Oliver , Thu Yee Mon 
Published: January 2020

ABSTRACTCancer results from complex interactions between genes that are misregulated. Although our understanding of the contribution of single genes to cancer is expansive, the interplay between genes in the context of this devastating disease remains to be understood. Using the Genomic Data Commons Data Portal through National Cancer Institute, we randomly selected ten data sets of breast cancer gene expression, acquired by RNA sequencing to be subjected to a computational method for the exploration of genetic interactions at a large scale. We focused on genes that suppress genome instability (GIS genes) since function or expression of these genes is often altered in cancer.In this paper, we show how to discover pairs of genes whose expressions demonstrate patterns of correlation. To ensure an inter-comparison across data sets, we tested statistical normalization approaches derived from the expression of randomly selected single housekeeping genes, or from the average of three. In addition, we systematically selected ten housekeeping genes for the purpose of normalization. Using normalized expression data, we determinedR2values from linear models for all possible pairs of GIS genes and presented our results using heatmaps.Despite the heterogeneity of data, we observed that multiple gene normalization revealed more consistent correlations between pairs of genes, compared to using single gene expressions. We also noted that multiple gene normalization using ten genes outperformed normalization using three randomly selected genes. Since this study uses gene expression data from cancer tissues and begins to address the reproducibility of correlation between two genes, it complements other efforts to identify gene pairs that co-express in cancer cell lines. In the future, we plan to define consistent genetic correlations by using gene expression data derived from different types of cancer and multiple gene normalization.CCS CONCEPTSApplied computing →Computational biology.ACM Reference FormatOliver Bonham-Carter and Yee Mon Thu. 2019. Systematic Normalization with Multiple Housekeeping Genes for the Discovery of Genetic Dependencies in Cancer. InNiagara Falls, New York.ACM, New York, NY, USA, 10 pages. https://doi.org/10.1145/nnnnnnn.nnnnnnn
Mutational effects on carbapenem hydrolysis of YEM-1, a new sub-class B2 metallo-β-lactamase fromYersinia mollaretii
Authors: Mercuri P. S. , Esposito R. , Blétard S. , Di Costanzo S. , Perilli M. , Kerff F. , Galleni M. 
Published: January 2020

ABSTRACTThe analysis of the genome sequence ofYersinia mollaretii(Y. mollaretii) ATCC 43969 indicates the presence of theblaYEMgene coding for YEM-1, a putative subclass B2 metallo-β-lactamase. The objectives of our work were to produce, purify and complete the kinetic characterization of YEM-1. Compared to the known subclass B2 metallo–β-lactamases, YEM-1 displayed a narrowest substrate profile since it is only able to hydrolyse imipenem with a high catalytic efficiency but not all the other carbapenems tested such as biapenem, meropenem, doripenem and ertapenem. A possible explanation of this peculiar activity profile is the presence of tyrosine 67 (loop L1), threonine 156 (loop L2) and serine 236 (loop L3) respectively. We showed that the substitution of Y67 broadened the activity profile of the enzyme for all carbapenems but still displayed a poor activity toward the other β-lactam classes.
LRRK2 and Rab10 Coordinate Macropinocytosis to Mediate Immunological Responses in Phagocytes
Authors: Liu Zhiyong , Xu Enquan , Zhao Hien Tran , Cole Tracy , West Andrew B. 
Published: January 2020

AbstractGenetic variation inLRRK2associates with susceptibility to Parkinson’s disease, Crohn’s disease, and mycobacteria infection, with high expression of LRRK2, and the LRRK2 kinase substrate Rab10, in phagocytic cells in the immune system. In mouse and human primary monocyte-derived macrophages, dendritic cells, and microglia-like cells, we find that Rab10 specifically regulates a specialized form of endocytosis known as macropinocytosis, without affecting phagocytosis or clathrin-mediated endocytosis. LRRK2 phosphorylates cytoplasmic PI(3,4,5)P3-positive GTP-Rab10 early macropinosomes, before EEA1 and Rab5 recruitment occurs. Macropinosome cargo in macrophages includes CCR5, CD11b, and MHCII, with LRRK2-phosphorylation of Rab10 potently blocking EHBP1L1-mediated recycling tubules and cargo turnover. EHBP1L1 over-expression competitively inhibits LRRK2-phosphorylation of Rab10, mimicking the effects of LRRK2 kinase inhibition in promoting cargo recycling. Both Rab10 knockdown and LRRK2 kinase inhibition potently suppresses the maturation of macropinosome-derived CCR5-loaded signaling endosomes important for CCL5-induced AKT-activation and chemotaxis. These data support a novel axis in the endolysosomal system whereby LRRK2-mediated Rab10 phosphorylation stalls vesicle fast-recycling to promote PI3K-AKT signal transduction.
The Effect of Kinship in Re-identification Attacks Against Genomic Data Sharing Beacons
Authors: Ayoz Kerem , Aysen Miray , Ayday Erman , Cicek A. Ercument 
Published: January 2020

AbstractMotivationBig data era in genomics promises a breakthrough in medicine, but sharing data in a private manner limits the pace of field. Widely accepted “genomic data-sharing beacon” protocol provides a standardized and secure interface for querying the genomic datasets. The data is only shared if the desired information (e.g., a certain variant) exists in the dataset. Various studies showed that beacons are vulnerable to re-identification (or membership inference) attacks. As beacons are generally associated with sensitive phenotype information, re-identification creates a significant risk for the participants. Unfortunately, proposed countermeasures against such attacks have failed to be effective, as they do not consider the utility of beacon protocol.ResultsIn this study, for the first time, we analyze the mitigation effect of the kinship relationships among beacon participants against re-identification attacks. We argue that having multiple family members in a beacon can garble the information for attacks since a substantial number of variants are shared among kinrelated people. Using family genomes from HapMap and synthetically-generated datasets, we show that having one of the parents of a victim in the beacon causes (i) significant decrease in the power of attacks and (ii) substantial increase in the number of queries needed to confirm an individual’s beacon membership. We also show how the protection effect attenuates when more distant relatives, such as grandparents are included alongside the victim. Furthermore, we quantify the utility loss due adding relatives and show that it is smaller compared to flipping based techniques.
Effect of cerium nitrate and salicylic acid on the titanium–zirconium chemical conversion coating of 6061 aluminum alloy
Authors: Wang Xiaobo , Zhan Wen , Gui Boyi 
Published: January 2020

Purpose
The purpose of this paper is to develop a chrome-free and phosphorus-free chemical conversion coating with good corrosion resistance, a novel chemical conversion coating was prepared by adding cerium nitrate hexahydrate and salicylic acid in the treatment solution containing titanium/zirconium ions on 6061 aluminum alloy.

Design/methodology/approach
Compared with the AA6061 aluminum alloy matrix, the self-corrosion potential of the conversion coating is significantly positively shifted, the self-corrosion current density is greatly reduced and its corrosion resistance is significantly improved. Morphology and composition of the conversion coatings were observed by scanning electron microscopy and energy-dispersive X-ray spectroscopy. The microdomain structure of conversion coatings at different formation stages was analyzed by electron probe microanalyzer.

Findings
An optimized preparation technique of titanium–zirconium chemical conversion coating for AA6061 aluminum alloy is obtained: H2TiF6 4 mL/L, H2ZrF6 0.4 mL/L, salicylic acid 0.35 g/L, Ce(NO3)3·6H2O 0.14 g/L, reaction temperature 30°C, reaction time 120 s and pH 4.0.

Originality/value
The coating forms on the Al(Fe)Si intermetallic compound, and Ce3+ is preferentially adsorbed on the intermetallic compound. The hydrolysis of Ce3+ causes the local pH of the solution to decrease, which promotes matrix dissolution and charge migration. As the microanode/microcathode reaction occurs, the local pH of the solution increases, and Al2O3/ZrO2/TiO2 begins to deposit on the surface of the metal substrate.
Enhancement of low-voltage ride-through capability of permanent magnet synchronous generator wind turbine by applying state-estimation technique
Authors: Shahriari Sayyed Ali Akbar , Mohammadi Mohammad , Raoofat Mahdi 
Published: January 2020

Purpose
The purpose of this study is to propose a control scheme based on state estimation algorithm to improve zero or low-voltage ride-through capability of permanent magnet synchronous generator (PMSG) wind turbine.

Design/methodology/approach
Based on the updated grid codes, during and after faults, it is necessary to ensure wind energy generation in the network. PMSG is a type of wind energy technology that is growing rapidly in the network. The control scheme based on extended Kalman filter (EKF) is proposed to improve the low voltage ride-through (LVRT) capability of the PMSG. In the control scheme, because the state estimation algorithm is applied, the requirement of DC link voltage measurement device and generator speed sensor is removed. Furthermore, by applying this technique, the extent of possible noise on measurement tools is reduced.

Findings
In the proposed control scheme, zero or low-voltage ride-through capability of PMSG is enhanced. Furthermore, the requirement of DC link voltage measurement device and generator speed sensor is removed and the amount of possible noise on the measurement tools is minimized. To evaluate the ability of the proposed method, four different cases, including short and long duration short circuit fault close to PMSG in the presence and absence of measurement noise are studied. The results confirm the superiority of the proposed method.

Originality/value
This study introduces EKF to enhance LVRT capability of a PMSG wind turbine.
Repositioning a country for global manufacturing competitiveness: a case of South Africa
Authors: Gates Gareth Earle , Adetunji Olufemi 
Published: January 2020

Purpose
This study aims to develop an artifact to measure the level of manufacturing competitiveness of a country in the global context and provide a suitable interpretation mechanism for the measured values, and to provide prescriptive solution where necessary so that the country can develop an actionable plan of program to move from the current level of global competitiveness to another such that they could provide more economic opportunities for their citizenry.

Design/methodology/approach
A manufacturing competitive index (MCI) was developed which includes relevant variables to capture a country’s manufacturing activity level in an economy with a balanced perspective. Reliable international sources were used. Ward algorithm was used to identify clear clusters of performance upon which competitive gaps were measured and improvement projects were identified and prioritized to obtain the best value for cluster transitional plan.

Findings
This study shows that the case country is not doing as well as it wants to believe, even when the relevant technology import measures were included in the expanded metric, but also, the next level of competitiveness is achievable within the national budget if proper prioritization is done.

Originality/value
The paper presents a cocktail of indexes that is more exhaustive of MCI, including both research capacity and technology import variables. It also uses clustering mechanism to provide a proper context to interpret the MCI scores in the context of peer nations. It presents a gap determination methodology and shows how priority projects could be logically selected to close measured gaps based on anticipated value from budget expenses
Public libraries and goal 16 of the SDGS in Cameroon: which actors for a national policy?
Authors: Ngo Kobhio Balôck Louise Lutéine 
Published: January 2020

Purpose
This paper aims to investigate how public libraries in Cameroon contribute to the achievement of goal 16 of the Sustainable Development Goals, which is “peace, justice and strong institutions”.

Design/methodology/approach
The methodology used is observation, talks with the librarians of public libraries from different regions of the country. Authorities in charge of this domain in Cameroon, that is the Ministry of Arts and Culture. Statistics from these different actors have been collected and used for the study, as well as those available through the National Institute of statistics.

Findings
The participation of public libraries in the implementation of goal 16 in Cameroon is remarkable, even if they lack substantial budgets for the achievement of their goals and rely partly on donations. The increasingly widespread use of information and communication technologies, as well as certain paying activities, partly compensates for this lack. Democracy, living together, access to employment and self-employment through the dissemination of information and animation are effective tools that public libraries implement. Users from different origins can gather and discuss freely on national issues. Public libraries are sometimes involved as mediators in the resolution of some social conflicts.

Practical implications
In a period were seeking peace, justice and strong institutions is one of the main national issues discussed in Cameroon, the study provides more visibility to both the authorities and the potential users on the important role played by public libraries in the resolution of national issues.

Originality/value
The study determines the different actors of a national policy in the framework of the creation, and the promotion of the public libraries and their respective roles for a more concerted and better-oriented action.
Career cooperation, coordination, compatibility and co-working
Authors: Shortland Susan 
Published: January 2020

Purpose
The purpose of this study is to examine how female expatriates mobilise couples’ dual-career coordination strategic choices to achieve their own and their partners’ desired career goals.

Design/methodology/approach
This qualitative research is based upon in-depth interviews with 20 dual-career female expatriates working in two case study oil and gas organisations.

Findings
Female expatriates use a series of tactics ranging from cooperation in maintaining a dual-career hierarchy, through to coordinating aspects of their own and their partners’ assignments, undertaking compatible industry roles and co-working (working together in the same organisation) to attempt to achieve a greater egalitarian international dual-career strategic outcome.

Research limitations/implications
This case analysis was based on a relatively small sample of female expatriates in heterosexual relationships working in oil and gas exploration. Further research in different sectors, with larger samples, and with male expatriates is also needed.

Practical implications
Employers should minimise periods of separation by focussing on coordinated assignment timings for both partners, facilitate suitable employment for both partners who wish to work abroad, and prioritise securing partner work visas.

Social implications
The inability to pursue desired dual-careers together while undertaking international assignments can be detrimental to couples’ relationships, potentially leading to unwillingness to expatriate and thereby deliver necessary skills in the host country.

Originality/value
The originality lies in identifying the tactics women use to enact dual-career coordination strategies, including coordinating assignment timings and locations to reduce separation and pursuing compatible roles to achieve egalitarian career and relationship outcomes. While women expected co-working in the same firm to facilitate dual-career mobility, its career outcomes were disappointing.
Service breakdown prevention
Authors: Woodside Arch G. , Caldwell Marylouise , Calhoun Jennifer Rebecca 
Published: January 2020

Purpose
This study defines service breakdowns, service breakdown prevention, and “servicide” as they relate to service-dominant logic. The study reviews relevant relevant literature on these three topics. This study categorizes real-life examples into five levels of dramatic turns toward service degradations and breakdowns that range from customer being aware but not mentioning service inadequacy to the service breakdown resulting in death of the customer or service provider. Taking initial steps in developing dramatic turn theory and improving the practice of service breakdown prevention are the major contributions of this study.

Design/methodology/approach
This study is a conceptual contribution that includes a dramatic turn role-playing exercise (at category 4 among five categories of dramatic turns for pedagogical/on-site enacting/practicing and training of service professionals. The study emphasizes and shows how to create and enact role-playing scenarios to increase requisite variety, provide training modules and increase skills/expertise in service enactment contexts.

Findings
Before explicit reviewing of the dramatic-turn performances, some of the participants as actors as well as audience members in role-play dramatic turns were quick to blame the customer behavior as the principal cause for the service breakdown. The study’s exposition stresses prevention of negative dramatic turns follows from experiencing and coaching a wide variety of customer and server interactions – achieving “richness” in enactments.

Research limitations/implications
Research on service breakdown prevention needs to include field experiments on the efficacy of training programs for effective management of dramatic turns.

Practical implications
Training of service workers and service managers in experiencing/participating in dramatic turns is likely to be beneficial in reducing the severe adverse outcomes and unintended consequences of service breakdowns. Prevention, not only service failure recovery, needs to be an explicit focus in hospitality management training and assessment.

Social implications
This study suggests tools and procedures to reduce the instances of the need for service breakdown recoveries.

Originality/value
The study calls attention and contributes a way forward in managing dramatic turns in hospitality service contexts. The study provides a nascent configurational theoretical foundation of dramatic-turn propositions. Given the severity of financial costs and loss of brand/firm reputation following the occurrence of extreme dramatic turns, a research focus on service breakdown prevention is necessary.
The mediating roles of gratitude and obligation to link employees’ social exchange relationships and prosocial behavior
Authors: Kim Haemi , Qu Hailin 
Published: January 2020

PurposeThis study aims to investigate the psychological mechanisms underlying hospitality employees’ social exchange relationships at work by applying the social aspects of work and the social exchange theory.Design/methodology/approachMTurk was used for conducting a cross-sectional questionnaire survey, targeting frontline employees who were working in full-service restaurants. Descriptive statistic, confirmatory factor analysis and structural equation modeling were performed.FindingsCustomer-employee exchange had a positive relationship with gratitude. Moreover, gratitude was positively associated with both role-prescribed customer service and extra-role customer service. Leader-member exchange and coworker exchange were positively related to obligation. Obligation had positive association with both role-prescribed customer service and extra-role customer service. The mediating effects of gratitude and obligation were statistically significant.Research limitations/implicationsEmployees’ social exchange relationship with customers promotes prosocial behaviors by arousing gratitude in them. Moreover, their social exchange relationships with supervisors and coworkers lead to prosocial behaviors by provoking obligation from them.Originality/valueThis research shows the importance of the social aspects of work to contribute to employees’ prosocial behavior in the hospitality industry. Moreover, it proves the critical roles of emotions to guide employees’ decisions about social exchange.
Financial development threshold, private capital inflows and economic growth
Authors: Anetor Friday Osemenshan 
Published: January 2020

Purpose
This study aims to examine the relationship between private capital inflows, financial development and economic growth in 28 sub-Saharan African (SSA) countries between the periods 1995 and 2017.

Design/methodology/approach
The study used a secondary source of data obtained from the world development indicator (WDI) and used the system generalized method of moments (SGMM) and dynamic panel threshold regression to analyze the data.

Findings
The study found that foreign direct investment has a negative and significant impact on the economic growth of SSA. The study also found that portfolio investment has a positive impact on economic growth but it is statistically insignificant. However, when portfolio investment interacted with financial development, it became positive and statistically significant presupposing that financial development is a necessary condition for portfolio investment to exert impact on economic growth. Further, the study showed that the interaction of foreign direct investment with financial development has a negative and significant effect on economic growth. Finally, the study found the minimum threshold of financial development at 42.66 per cent.

Practical implications
Policymakers in SSA should be cautious and critical in the kind of foreign direct investment they attract as the open door policy to attract all kinds of foreign direct investment would not bring about the desired result. Also, policymakers in the region should develop and implement policies that would deepen and strengthen the financial system to foster the development of the country’s financial sector and accelerate economic growth.

Originality/value
The contribution of the study lies in establishing a minimum threshold of financial development; thus, providing a clear-cut direction for policymakers in SSA countries in their pursuit of financial development and economic growth.