bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibacterial effects of chitosan coating containing Mentha aquatica L. essence against Escherichia coli, Staphylococcus aureus and Listeria monocytogenes in Iranian white cheese
Authors: Hani Tabaie Zavareh Seyed Ahmad , Ardestani Fatemeh 
Published: January 2020

The effect of a chitosan coating and Mentha aquatica L. essence on Iranian white cheese was investigated. Results showed 100% inhibition of Escherichia coli growth using 1.5% essence after 10 days. After 15 days of incubation, the Staphylococcus aureus population was reduced by 44.2%, 70.0%, and 88.5% using 0.5, 1.0 and 1.5% essence, respectively. After 15 days, Listeria monocytogenes growth was inhibited by 63.84%, 70.12%, and 85.9% using 0.5, 1.0, and 1.5% essence, respectively. Inhibition zone diameter studies also confirmed the antibacterial effects of applied coating against all the above‐mentioned bacteria in Iranian white cheese.
Local labour market competition and radical right voting: Evidence from France
Authors: BOLET DIANE 
Published: January 2020

AbstractHow do the economic effects of immigration affect radical right support? The evidence in support of the labour market competition theory — which posits that the economic threat posed by immigration to jobs and wages leads to radical right voting — has been mixed. On the one hand, individual‐level surveys underreport economic drivers because of social desirability bias. On the other hand, contextual studies show contradictory findings due to an over‐reliance on units of analysis that are too aggregated to meaningfully capture the competitive threat posed by immigrants. This paper identifies the influence of labour market competition on radical right voting at a local level in contexts where native workers are directly affected by the arrival of immigrants who have similar or higher skillsets. Using an original longitudinal dataset of fine‐grained municipal electoral, demographic and economic data from France over the 2002–2017 period, the paper provides empirical evidence of local contextual influences of economic competition between natives and immigrants of any skillset. Under local conditions of material deprivation, measured by the local unemployment rate, the effect of labour market competition on municipalities’ radical right vote share is amplified. Moreover, higher radical right support is observed in municipalities with a higher share of any one of the following groups: low‐skilled natives, medium‐skilled immigrants or high‐skilled immigrants. This supports the hypothesis that immigrants with higher qualifications are compelled to accept lower‐skilled jobs, and are thus perceived as a competitive threat to low‐skilled natives. By reconciling radical right contextual studies and research on the political economy of immigration policies, this paper highlights the importance of a local analysis in detecting the effect of labour market competition on radical right support. This paper also explains why some local areas are more prone to radical right support than others over time.
Genome‐wide association study for growth and fatness traits in Chinese Sujiang pigs
Authors: Xu P. , Ni L. , Tao Y. , Ma Z. , Hu T. , Zhao X. , Yu Z. , Lu C. , Zhao X. , Ren J. 
Published: January 2020

SummaryGrowth and fatness traits are complex and economically important traits in the pig industry. The molecular basis underlying porcine growth and fatness traits remains largely unknown. To uncover genetic loci and candidate genes for these traits, we explored the GeneSeek GGP Porcine 80K SNP chip to perform a GWAS for seven growth and fatness traits in 365 individuals from the Sujiang pig, a recently developed breed in China. We identified two, 17, one and 11 SNPs surpassing the suggestively significant threshold (P < 1.86 × 10−5) for body weight, chest circumference, chest width and backfat thickness respectively. Of these SNPs, 20 represent novel genetic loci, and five and four SNPs were respectively associated with chest circumference and backfat thickness at a genome‐wide significant threshold (P < 9.31 × 10−7). Eight SNPs had a pleiotropic effect on both chest circumference and backfat thickness. The most remarkable locus resided in a region between 72.95 and 76.27 Mb on pig chromosome 4, harboring a number of previously reported quantitative trait loci related to backfat deposition. In addition to two reported genes (PLAG1 and TAS2R38), we identified four genes including GABRB3, ZNF106, XKR4 and MGAM as novel candidates for body weight and backfat thickness at the mapped loci. Our findings provide insights into the genetic architecture of porcine growth and fatness traits and potential markers for selective breeding of Chinese Sujiang pigs.
Methamphetamine Use and Sexual Risk Behaviors among Men Who Have Sex With Men in a Mexico‐US Border City
Authors: Loza Oralia , Curiel Zuleika V. , Beltran Oscar , Ramos Rebeca 
Published: January 2020

Background and ObjectivesMethamphetamine (meth) use and its related risk behaviors for HIV among men who have sex with men (MSM) are a public health concern across the Mexico‐US border. This study aims to contribute to the limited literature of meth use and sexual risk behaviors among Latino MSM on the Mexico‐US border.MethodsData were drawn from the Meth Pilot Study (2014‐2015) among men who use meth (n = 100). Descriptive statistics and bivariate analysis comparing MSM to non‐MSM were conducted using Pearson’sχ2test, Fisher’s exact tests, and Mann‐WhitneyUtest; all tests were conducted using SPSS v.25.ResultsMost participants obtained meth in El Paso, Texas (87.2%), used meth orally (65.2%) or smoked (78.3%), and the most common reason for initiation was curiosity. Significant differences (P < .05) in meth use behaviors and sexual risk behaviors between MSM and non‐MSM who used meth included: median number of sex partners (7 vs 3), being penetrated anally by last sexual partner (31.6% vs 1.4%), and engaging in transactional sex ever (63.2% vs 9.6%) and past 12 months (52.6% vs 6.8%). Finally, rates of HIV positivity were higher among MSM than non‐MSM (10.5% vs 1.4%).Discussion and ConclusionsAmong men who use meth, MSM are engaging in higher HIV risk behaviors compared with non‐MSM. Understanding these risks could help identify candidates for pre‐exposure prophylaxis (PrEP) and evidence‐based substance use disorder treatment options.Scientific SignificanceThis study reveals that Latino MSM who use meth is a high‐risk group for HIV and a need for tailored interventions. (Am J Addict 2020;29:111–119)
Systematic review with meta‐analysis: the risks of proton pump inhibitors during pregnancy
Authors: Li Cheng Mei , Zhernakova Alexandra , Engstrand Lars , Wijmenga Cisca , Brusselaers Nele 
Published: January 2020

SummaryBackgroundThere have been safety concerns considering long‐term proton pump inhibitor (PPI) use, also during pregnancy.AimsTo assess the risk of adverse neonatal outcomes associated with maternal intake of PPIs by means of systematic review and meta‐analysis.MethodsThe systematic search included PubMed, Web of Science, Cochrane Database and Embase (inception until June 2019). All studies reporting ≥1 adverse pregnancy outcome comparing PPI users to non‐users. Histamine‐2 receptor antagonists (H2RA) were also compared to both non‐users and PPI users. Outcomes included congenital malformations, abortion, stillbirth, neonatal death, preterm birth, small for gestational age and low birth weight. Pooled odds ratios (OR) and 95% confidence intervals (CI) were obtained by random‐effects modelling. PROSPERO study‐protocol: CRD42018103320.ResultsIn total, 26 observational studies (20 cohort, 6 case‐control studies) were identified, of which 19 assessed PPIs and 12 H2RA. PPI use was associated with an increased risk of congenital malformations (OR 1.28, 95% CI 1.09‐1.52), especially in case‐control studies (OR 2.04, 1.46‐2.86). No associations were found between H2RA and congenital malformations. No significant associations were found between PPI use and abortions, stillbirth, neonatal death, preterm birth and low‐birth weight, although H2RA use may be associated with an increased risk of preterm birth (OR 1.25, 95% CI 1.02‐1.56). Although statistical heterogeneity and the risk of bias were overall low, clinical heterogeneity, information and selection bias may be present in the individual studies.ConclusionsThis meta‐analysis suggests an association between maternal PPI use and congenital malformations in humans, yet power was insufficient to assess specific malformations and drugs.
Off‐clamp tumor excision using soft coagulation in laparoscopic and robotic partial nephrectomy
Authors: Yoshida Tetsuya , Okinaka Yuki , Tomita Keiji , Tsuru Teruhiko , Kageyama Susumu , Narita Mitsuhiro , Kawauchi Akihiro 
Published: January 2020

AbstractIntroductionThe aim of this study was to assess the perioperative outcomes of off‐clamp tumor excision using soft coagulation in laparoscopic and robotic partial nephrectomy.MethodsWe retrospectively analyzed the data from 78 patients who underwent minimally invasive partial nephrectomy, using soft coagulation, at the Shiga University of Medical Science, between September 2013 and April 2017. Tumor excision and hemostasis without renal arterial clamping was performed using soft coagulation. Collecting system repair and renorrhaphy with arterial clamping were carried out only if the collecting system had been opened.ResultsForty‐three of the 78 patients underwent laparoscopic partial nephrectomy using soft coagulation and the other 35 patients underwent robotic partial nephrectomy using soft coagulation. The median estimated total blood loss was 73 (0‐1140) mL and no patient needed a blood transfusion. No cases featured postoperative hemorrhagic events. Six patients with urinary fistula needed prolonged ureteral stenting. The median percentage change of the estimated glomerular filtration rate was −7.2 at one to 3 months after surgery.ConclusionThe off‐clamp soft coagulation technique in laparoscopic partial nephrectomy and robotic partial nephrectomy is a safe and feasible approach to excise kidney tumors. This procedure may reduce the incidence of perioperative hemorrhagic complications.
The potential function of endometrial‐secreted factors for endometrium remodeling during the estrous cycle
Authors: Billhaq Dody Houston , Lee Sang‐Hee , Lee Seunghyung 
Published: January 2020

AbstractUterine has a pivotal role in implantation and conceptus development. To prepare a conducive uterine condition for possibly new gestation during the estrous cycle, uterine endometrium undergoes dramatic remodeling. In addition, angiogenesis is an indispensable biological process of endometrium remodeling. Furthermore, essential protein expressions related to important biological processes of endometrium remodeling, which are vascular endothelial growth factor (VEGF), myoglobin (MYG), collagen type IV (COL4), fucosyltransferase IV (FUT4), and cysteine‐rich protein 2 (CRP2), were detected in the endometrial tissue reported in many previous studies and recently discovered in histotroph substrates during the estrous cycle. Those proteins, which are liable for provoking new vessel development, cell proliferation, cell adhesion, and cell migration, were expressed higher in the histotroph during the luteal phase than follicular phase. Histotroph proteins considerably contribute to endometrium remodeling during the estrous cycle. To that end, the following review will discuss and highlight the relevant information and evidence of the uterine fluid proteins as endometrial‐secreted factors that adequately indicate the potential role of the uterine secretions to be involved in the endometrial remodeling process.
Efficient recruitment of c‐FLIPL to the death‐inducing signaling complex leads to Fas resistance in natural killer‐cell lymphoma
Authors: Masuda Azuchi , Isobe Yasushi , Sugimoto Koichi , Yoshimori Mayumi , Arai Ayako , Komatsu Norio 
Published: January 2020

AbstractActivation‐induced cell death (AICD) mediated by the Fas/Fas ligand (FasL) system plays a key role in regulating immune response. Although normal natural killer (NK) cells use this system for their homeostasis, malignant NK cells seem to disrupt the process. Extranodal NK/T‐cell lymphoma, nasal type (ENKL) is a rare but fatal disease, for which novel therapeutic targets need to be identified. We confirmed that ENKL‐derived NK cell lines NK‐YS and Hank1, and primary lymphoma cells expressed procaspase‐8/FADD‐like interleukin‐1β‐converting enzyme (FLICE) modulator and cellular FLICE‐inhibitory protein (c‐FLIP), along with Fas and FasL. Compared with Fas‐sensitive Jurkat cells, NK‐YS and Hank1 showed resistance to Fas‐mediated apoptosis in spite of the same expression levels of c‐FLIP and the death‐inducing signaling complex (DISC) formation. Unexpectedly, the long isoform of c‐FLIP (c‐FLIPL) was coimmunoprecipitated with Fas predominantly in both ENKL‐derived NK cell lines after Fas ligation. Indeed, c‐FLIPL was more sufficiently recruited to the DISC in both ENKL‐derived NK cell lines than in Jurkat cells after Fas ligation. Knockdown of c‐FLIPL per se enhanced autonomous cell death and restored the sensitivity to Fas in both NK‐YS and Hank1 cells. Although ENKL cells are primed for AICD, they constitutively express and efficiently utilize c‐FLIPL, which prevents their Fas‐mediated apoptosis. Our results show that c‐FLIPL could be a promising therapeutic target against ENKL.
Phenotypic shift of small intestinal intra-epithelial type 1 innate lymphoid cells in celiac disease is associated with enhanced cytotoxic potential
Authors: Uhde M , Yu X , Bunin A , Brauner C , Lewis S K , Lebwohl B , Krishnareddy S , Alaedini A , Reizis B , Ghosh S , Green P H , Bhagat G 
Published: January 2020

Summary
The small intestinal (SI) epithelium harbors a heterogeneous population of lymphocytes that mediate mucosal damage and repair in celiac disease (CD). The composition and roles of human proximal SI intra-epithelial innate lymphoid cells (ILCs), and their alterations in CD, are not well understood. We report that duodenal intra-epithelial ILCs predominantly consist of natural killer (NK)p44+CD127− cytotoxic ILC1s and NKp44−CD127+ helper ILC1s, while ILC3s only represent a minor population. In patients with newly diagnosed or active CD (ACD) and refractory CD type 1 (RCD I), the frequency of SI NKp44+ ILCs is decreased, with restoration of NKp44+ ILC frequency observed in patients adhering to a gluten-free diet who show evidence of mucosal healing. Moreover, the frequency of SI NKp44− ILCs is increased in ACD and RCD I patients and correlates with the severity of villous atrophy and epithelial damage, as assessed by serum levels of fatty acid binding protein 2 (FABP2). We show that the ILC alterations in CD represent a phenotypic shift of cytotoxic ILC1s rather than an increase in helper ILC1s or transdifferentiation of ILC1s to ILC3s, and activation-induced loss of NKp44 by cytotoxic ILC1s is associated with increased interferon (IFN)-γ expression and release of lytic granules. These findings suggest that intra-epithelial NKp44−CD127− cytotoxic ILC1s may contribute to mucosal damage in CD.
Further insights into the treatment of perineal hernia based on a the experience of a single tertiary centre
Authors: Blok R. D. , Brouwer T. P. A. , Sharabiany S. , Musters G. D. , Hompes R. , Bemelman W. A. , Tanis P. J. 
Published: January 2020

AbstractAimThere is little evidence concerning the optimal surgical technique for the repair of perineal hernia. This study aimed to report on the evolution of a technique for repair of perineal hernia by analysing the experience in a tertiary referral centre.MethodThis was a retrospective review of consecutive patients who underwent perineal hernia repair after abdominoperineal excision in a tertiary referral centre. The main study end‐points were rate of recurrent perineal hernia, perineal wound complications and related re‐intervention.ResultsThirty‐four patients were included: in 18 patients a biological mesh was used followed by 16 patients who underwent synthetic mesh repair. Postoperative perineal wound infection occurred in two patients (11%) after biological mesh repair compared with four (25%) after synthetic mesh repair (P = 0.387). None of the meshes were explanted. Recurrent perineal hernia following biological mesh was found in 7 of 18 patients (39%) after a median of 33 months. The recurrence rate with a synthetic mesh was 5 of 16 patients (31%) after a median of 17 months (P = 0.642). Re‐repair was performed in four (22%) and two patients (13%), respectively (P = 0.660). Eight patients required a transposition flap reconstruction to close the perineum over the mesh, and no recurrent hernias were observed in this subgroup (P = 0.030). No mesh‐related small bowel complications occurred.ConclusionRecurrence rates after perineal hernia repair following abdominoperineal excision were high, and did not seem to be related to the type of mesh. If a transposition flap was added to the mesh repair no recurrences were observed, but this finding needs confirmation in larger studies.
Extracorporeal photopheresis for graft‐vs‐host disease: A literature review and treatment guidelines proposed by the Nordic ECP Quality Group
Authors: Nygaard Marietta , Wichert Stina , Berlin Gösta , Toss Fredrik 
Published: January 2020

AbstractExtracorporeal photopheresis (ECP) is one of the most used and established therapies for steroid‐refractory graft‐vs‐host disease (GvHD), with a good effect to side effect profile. In this review, we present a summary of present literature and provide evidence‐based treatment guidelines for ECP in GvHD. The guidelines constitute a consensus statement formed by the Nordic ECP Quality Group representing all ECP centres in the Nordic countries, and aims to facilitate harmonisation and evidence‐based practice. In developing the guidelines, we firstly conducted a thorough literature search of original articles and existing guidelines. In total, we identified 26 studies for ECP use in acute GvHD and 36 in chronic GvHD. The studies were generally small, retrospective and heterogeneous regarding patient characteristics, treatment schedule and outcome assessment. In general, a majority of patients achieved partial response or better, but response rates varied by the organs affected. Head‐to‐head comparisons to other treatment modalities were lacking. Overall, we consider the quality of evidence to be low–moderate (GRADE) and encourage future prospective multi‐armed trials to strengthen the present recommendations. However, despite limitations in evidence strength, standardised treatment schedules and regular follow‐up are imperative to ensure the best possible patient outcome.
Investigating cortical features of Sotos syndrome using mice heterozygous for Nsd1
Authors: Oishi Sabrina , Zalucki Oressia , Vega Michelle S. , Harkins Danyon , Harvey Tracey J. , Kasherman Maria , Davila Raul A. , Hale Lauren , White Melissa , Piltz Sandra , Thomas Paul , Burne Thomas H. J. , Harris Lachlan , Piper Michael 
Published: January 2020

AbstractSotos syndrome is a developmental disorder characterized by a suite of clinical features. In children, the three cardinal features of Sotos syndrome are a characteristic facial appearance, learning disability and overgrowth (height and/or head circumference > 2 SDs above average). These features are also evident in adults with this syndrome. Over 90% of Sotos syndrome patients are haploinsufficient for the gene encoding nuclear receptor‐binding Su(var)3‐9, Enhancer‐of‐zesteand Trithorax domain‐containing protein 1 (NSD1). NSD1 is a histone methyltransferase that catalyzes the methylation of lysine residue 36 on histone H3. However, although the symptomology of Sotos syndrome is well established, many aspects of NSD1 biology remain unknown. Here, we assessed the expression of Nsd1 within the mouse brain, and showed a predominantly neuronal pattern of expression for this histone‐modifying factor. We also generated a mouse strain lacking one allele of Nsd1 and analyzed morphological and behavioral characteristics in these mice, showing behavioral characteristics reminiscent of some of the deficits seen in Sotos syndrome patients.
Update on the use of dapsone in dermatology
Authors: Ghaoui Nohra , Hanna Edith , Abbas Ossama , Kibbi Abdul‐Ghani , Kurban Mazen 
Published: January 2020

AbstractDapsone (4,4'‐diaminodiphenylsulfone) is the only remaining sulfone used in anthropoid therapeutics and is commercially available as an oral formulation, an inhaled preparation, and a 5% or 7.5% cream. Dapsone has antimicrobial effects stemming from its sulfonamide‐like ability to inhibit the synthesis of dihydrofolic acid. It also has anti‐inflammatory properties such as inhibiting the production of reactive oxygen species, reducing the effect of eosinophil peroxidase on mast cells and down‐regulating neutrophil‐mediated inflammatory responses. This allows for its use in the treatment of a wide variety of inflammatory and infectious skin conditions. Currently in dermatology, the US Food and Drug Administration (FDA)‐approved indications for dapsone are leprosy, dermatitis herpetiformis, and acne vulgaris. However, it proved itself as an adjunctive therapeutic agent to many other skin disorders. In this review, we discuss existing evidence on the mechanisms of action of dapsone, its FDA‐approved indications, off‐label uses, and side effects.
Krüppel homologue 1 interacts directly with Hairy and regulates ecdysis in the brown planthopper
Authors: Mao Y. , Li Y. , Gao H. , Lin X. 
Published: January 2020

AbstractJuvenile hormone (JH) plays important roles in the growth and development of insects. JH and its receptor methoprene‐tolerant (Met) regulate the expression of transcription factors to control the transcription of downstream genes. The expression of Hairy (Hry) and Krüppel homologue 1 (Kr‐h1) is regulated by JH and JH receptors. Hry and Kr‐h1 are both crucial in mediating JH signalling. However, whether they interact at the gene level in regulating metamorphosis and whether they interact physically at the protein level remain unknown. We used co‐immunoprecipitation, glutathione S‐transferase pull‐down and RNA interference (RNAi) approaches to study the genetic and biochemical interactions of the two proteins Hry and Kr‐h1. The results showed that brown planthopper (Nilaparvata lugens) Hry and Kr‐h1 interact directly: Hry binds to the N‐terminal of Kr‐h1, which includes five zinc‐finger domains. The RNAi experiment showed that downregulation of Hry reduced the ratio of ecdysis failure caused by knockdown of Kr‐h1, indicating that the downregulation of Hry might mitigate ecdysis failure via the downregulation of Kr‐h1. The expression of Hry increased significantly when Kr‐h1 was downregulated, whereas it did not change significantly when both were downregulated. Our results suggest that the binding of Hry protein with Kr‐h1 prevents the N‐terminal five zinc‐finger domains from binding with DNA, which in turn inactivates the transcription activator or inhibitor function of Kr‐h1. Hry could possibly be used as a target for pesticide applications in the future.