bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dopamine D2-Like Receptors Modulate Intrinsic Properties and Synaptic Transmission of Parvalbumin Interneurons in the Mouse Primary Motor Cortex
Authors: Cousineau Jérémy , Lescouzères Léa , Taupignon Anne , Delgado-Zabalza Lorena , Valjent Emmanuel , Baufreton Jérôme , Le Bon-Jégo Morgane 
Published: April 2020

AbstractDopamine (DA) plays a crucial role in the control of motor and higher cognitive functions such as learning, working memory, and decision making. The primary motor cortex (M1), which is essential for motor control and the acquisition of motor skills, receives dopaminergic inputs in its superficial and deep layers from the midbrain. However, the precise action of DA and DA receptor subtypes on the cortical microcircuits of M1 remains poorly understood. The aim of this work was to investigate in mice how DA, through the activation of D2-like receptors (D2Rs), modulates the cellular and synaptic activity of M1 parvalbumin-expressing interneurons (PVINs) which are crucial to regulate the spike output of pyramidal neurons (PNs). By combining immunofluorescence,ex vivoelectrophysiology, pharmacology and optogenetics approaches, we show that D2R activation increases neuronal excitability of PVINs and GABAergic synaptic transmission between PVINs and PNs in Layer V of M1. Our data reveal how cortical DA modulates M1 microcircuitry, which could be important in the acquisition of motor skills.
Divergence of Peroxisome Membrane Gene Sequence and Expression Between Yeast Species
Authors: Dubin Claire A , Roop Jeremy I , Brem Rachel B 
Published: April 2020

Abstract
Large population-genomic sequencing studies can enable highly-powered analyses of sequence signatures of natural selection. Genome repositories now available for Saccharomyces yeast make it a premier model for studies of the molecular mechanisms of adaptation. We mined the genomes of hundreds of isolates of the sister species S. cerevisiae and S. paradoxus to identify sequence hallmarks of adaptive divergence between the two. From the top hits we focused on a set of genes encoding membrane proteins of the peroxisome, an organelle devoted to lipid breakdown and other specialized metabolic pathways. In-depth population- and comparative-genomic sequence analyses of these genes revealed striking divergence between S. cerevisiae and S. paradoxus. And from transcriptional profiles we detected non-neutral, directional cis-regulatory variation at the peroxisome membrane genes, with overall high expression in S. cerevisiae relative to S. paradoxus. Taken together, these data support a model in which yeast species have differentially tuned the expression of peroxisome components to boost their fitness in distinct niches.
Estudo sobre fatores intrínsecos a paciente que influenciam na dose glandular média em exames de tomossíntese de mama
Authors: Engler Camila , Attie Márcia Regina Pererira , Reis Lucas Paixão , Squair Peterson Lima , Chevalier Margarita , Nogueira Maria do Socorro 
Published: April 2020

RESUMOObjetivo: A dose glandular média (DGM) é a grandeza dosimétrica utilizada para estimar a dose de radiação à qual um paciente é exposta ao realizar um exame de mamografia. Este estudo tem como objetivo verificar a relação entre os fatores característicos do paciente e a DGM. Materiais e Métodos: As imagens de tomossíntese mamária de 660 pacientes foram analisadas usando o software Volpara. A partir desta análise, obteve-se a densidade volumétrica da mama (DVM) e a DGM. A idade do paciente e a espessura da mama comprimida (EMC) foram obtidas do cabeçalho DICOM das imagens. Utilizando o software estatístico SPSS, foram realizados testes de correlação entre a DVM, a idade do paciente e a TCC com a DGM, e calculados os respectivos coeficientes de correlação (r). Resultados: A amostra de pacientes teve uma média da EMC de 59 ± 12mm, mediana da idade da paciente de 51 anos (25 a 87 anos), mediana da DGD de 1,53 mGy (0,43 a 4,68 mGy) e mediana da DVM de 8% ( Intervalo de 2,16% a 36,89%). Os coeficientes de correlação calculados foram 0,695 para a relação entre DGM e EMC, 0,095 para a relação entre DGM e DVM e -0,280 para a relação entre DGM e a idade da paciente. Conclusão: Os resultados mostram que a EMC é o fator mais relacionado ao aumento da DGM. DVM é o fator com menor impacto e a idade da paciente tem uma relação inversa com a DGM. ABSTRACTObjective: Mean Glandular Dose (MGD) is the dosimetric quantity used to estimate the radiation dose to which a patient is exposed when performing a mammography examination. This study aims to verify the relationship between patient characteristic factors and MGD. Materials and Methods: Breast tomosynthesis images of 660 patients were analyzed using Volpara software. From this analysis, the volumetric breast density (VBD) and the MGD were obtained. Patient age and compressed breast thickness (CBT) were obtained from the DICOM header of the images. Using the SPSS statistical software, correlation tests were performed between the VBD, patient age and CBT with the MGD, and the respective correlation coefficients (r) were calculated. Results: The patient sample had a mean CBT of 59 (± 12mm), median patient age of 51 years (25 to 87 years range), median MGD of 1.53 mGy (0.43 to 4.68 mGy range), and median VBD of 8% (2.16% to 36.89% range). The calculated correlation coefficients were 0.695 for the relationship between MGD and CBT, 0.095 for the relationship between MGD and VBD, and -0.280 for the relationship between MGD and patient age. Conclusion: The results show that CBT is the factor most related to the MGD increase. VBD is the factor with the least impact and the patient's age has an inverse relationship with the MGD.
Breastfeeding and Mortality Under 2 Years of Age in Sub-Saharan Africa
Authors: Zhao Min , Wu Han , Liang Yajun , Liu Fangchao , Bovet Pascal , Xi Bo 
Published: April 2020

BACKGROUND:
Several studies have investigated the association of breastfeeding status with offspring mortality in Africa, but most studies were from one center only or had limited statistical power to draw robust conclusions.

METHODS:
Data came from 75 nationally representative cross-sectional Demographic and Health Surveys in 35 countries in sub-Saharan Africa conducted between 2000 and 2016. Our study relied on 217 112 individuals aged 4 days to 23 months for breastfeeding pattern analysis, 161 322 individuals aged 6 to 23 months for breastfeeding history analysis, and 104 427 individuals aged 12 to 23 months for breastfeeding duration analysis.

RESULTS:
Compared with children aged 4 days to 23 months exclusively breastfed in the first 3 days of life, those not breastfed had a high risk of mortality at &lt;2 years of age (odds ratio [OR] = 13.45; 95% confidence interval [CI] = 11.43–15.83). Young children who were predominantly breastfed or partially breastfed had moderately increased risk of mortality at &lt;2 years of age (OR = 1.11, 95% CI = 1.03–1.21 for predominant pattern; OR = 1.12, 95% CI = 0.99–1.27 for partial pattern). Compared with children aged 6 to 23 months who were breastfed within the first 6 months of life, those not breastfed had a high risk of mortality (OR = 5.65; 95% CI = 4.27–7.47). Compared with children aged 12 to 23 months who were breastfed for ≥6 months, those who were breastfed for shorter periods had a higher risk of mortality (OR = 2.78, 95% CI = 1.45–5.32 for duration of &lt;3 months; OR = 5.28, 95% CI = 3.24–8.61 for those who were not breastfed).

CONCLUSIONS:
Our findings support exclusive breastfeeding during the first 6 months of life and continued breastfeeding up to 2 years of age recommended by the World Health Organization for reducing mortality of children &lt;2 years old in sub-Saharan Africa.
The association between sympatho-vagal balance and central blood pressures
Authors: Latchman P. L. , Gates G. , Pereira J. , Axtell R R. , Gardner K. , Schlie J. , Yang Q. , Yue T. , Morin-Viall A. , DeMeersman R. 
Published: April 2020

AbstractPurposeHigh central blood pressure is more predictive of cardiovascular disease (CVD) versus high peripheral blood pressure. Measures of central pressures (CPs) include, central systolic blood pressure (CSBP) and central diastolic blood pressure. Measures of central pressures augmentation (CPsA) include augmentation pressure (AP) and the augmentation index @ 75 beats·min−1 (AIx@75). Increased sympathetic tone (ST) is also associated with CVD. The low to high frequency ratio (LF/HF) is often used to determine sympatho-vagal balance. Given the association between ST, CPs, CPsA and CVD there is a need to understand the association between these predictors of CVD. The aims of this study were to examine the association between the LF/HF ratio, CPs, and CPsA in men and women collectively and based on gender.MethodsWe measured the LF/HF ratio, CSBP, AP, and AIx@75 in 102 participants (41F/61M). The LF/HF ratio was determined via power spectral density analysis. CSBP, AP, and AIx@75 were determined via applanation tonometry.ResultsThe LF/HF ratio was inversely associated with AP (r = –0.26) and AIx @75 (r = –0.29) in the combined group of men and women. The LF/HF ratio was inversely associated with CSBP (r = –0.27), AP (r = –0.28), and AIx@75 (r = –0.32) in men, but not in women.ConclusionThere is an inverse association between the LF/HF ratio, AP, and AIx@75 in men and women combined. The association between the LF/HF ratio, CSBP, AP, and AIx@75, differs based on gender.
The stability of sample storage for complete blood count (CBC) toward the blood cell morphology
Authors: Rahmanitarini Arie , Hernaningsih Yetti , Indrasari Yulia Nadar 
Published: April 2020

Background: Peripheral blood smear examination had a pivotal role in determining diagnosis and as confirmation of the automatic hematology analyzer results. The storage process was highly influential on the morphological stability of the cell. This study aimed to assess the morphological changes in blood cells stored at certain period and temperature. Method: Sample of 30 blood specimens of healthy people with dipotassium ethylenediaminetetraacetic acid (K2EDTA) anticoagulants. The specimens were stored at room temperature (18-25ÂºC) and refrigerator temperature (2-8ÂºC) and were analyzed at the preliminary examination i.e., 8 hours, 16 hours, 24 hours, 48 hours, 72 hours, and 96 hours. Kappa test was used in validating the reading of PBS (Peripheral Blood Smear). The discrimination testing used were paired t-test and Kolmogorov Smirnov test with p value <0.005, which stated to be significant.Â Result: The changes in erythrocytes morphology stored at room temperature (18-25ÂºC) was that the erythrocytes crenation started to happen at 8 hours storage with the grading scale of +2 that were found in 24 (80%) samples, whereas at refrigerator temperature (2-8ÂºC) the grading scale was +1 and found in 13 (43.3%) samples. Spherocytes on erythrocytes began to form at room temperature (18-25ÂºC) at 8 hours storage with grading scale of +1 and was found in 2 (6.7%) samples, whereas at refrigerator temperatures (2-8ÂºC) spherocytes on erythrocytes began to form at 24 hours storage with grading scale of + 2 and was found in 3 (10%) samples.Conclusion: Peripheral blood smear (PBS) examination shall be done immediately to obtain significant results.
Erythrocyte alloantibodies in chronic kidney disease patients receiving packed red cell transfusions in Sanglah General Hospital, Denpasar
Authors: Krisnawati Ni Komang , Herawati Sianny , Mulyantari Ni Kadek 
Published: April 2020

Introduction: In Chronic kidney disease (CKD) patients, as the consequences of the pathogenesis, anemia due to the failure of erythropoietin production requires Packed Red Cell (PRC) transfusion. The presence of alloantibodies in CKD patients who routinely require transfusion in Bali has never been evaluated. This study aims to determine the erythrocyte alloantibodies of CKD patients who received PRC transfusions in Sanglah Hospital.Methods: This study was a cross sectional descriptive study in adult CKD patients with history of PRC transfusion more than twice.Results: A total of 50 patients with Chronic Kidney Disease consisting of 31 men and 19 women, mostly 46-65 years old (60%) and the most blood group were blood group O (44%). There were 3 patients with positive antibody screening and identification results, 2 male patients (66.67%) and 1 female (33.33%). Two patients 46-65 years (66.67%) while 1 person 18-45 years (33.33%), with 2 person with blood group A (66.67%) and one person with blood group O (33.37% ). These three patients presented multiple alloantibodies in the form of anti-K, anti-Kpa, anti-LuaConclusion: From Â the CKD patients who received PRC transfusions in Sanglah Hospital, Â±6% formed alloantibodies. All of the alloantibodies formed were multiple alloantibodies, in the form of anti-K, anti-Kpa and Lua.
The relationship of lactic acid, neutrophil lymphocyte ratio and monocyte lymphocyte ratio with the outcomes of coronary arterial disease patients following coronary artery bypass surgery
Authors: Citradinata Cynthia , Widyastiti Nyoman Suci 
Published: April 2020

Background: Coronary artery bypass surgery (CABG) is a surgical procedure for coronary arterial disease (CAD). It has a risk of postoperative complication, extending from a long length of stay in the intensive care unit (ICU) to death. CABG needs a long duration of perioperative time, which decreases tissue oxygenation. It causes increased lactic acid production and initiates the secretion of inflammatory factors. Neutrophil/lymphocyte ratio (NLR) and monocyte/lymphocyte ratio (MLR) become inflammatory signs that are caused by the failure of heart microcirculation during the operative procedure. This study aimed to determine the relationship of lactic acid, NLR and MLR with the outcomes of CAD patients following CABG surgery.Methods: This was a prospective study including 42 CAD patients who had undergone CABG procedure at Dr. Kariadi Hospital Semarang between December 2017 and July 2018. Lactic acid data were taken using the spectrophotometer method. Â NLR and MLR were taken by the calculation index. The outcomes data were ICU length of stay (LoS), complications, and death, which were obtained from the medical record. The data were analyzed using bivariate analysis.Results: There was a significant correlation between lactic acid with ICU LoS (p = 0.001), complication (p = 0.01), and mortality (p = 0.044). There was a significant correlation between NLR with ICU LoS (p = 0.003), complication (p = 0.009), and mortality (p = 0.014). There was a significant correlation between MLR with ICU LoS (p = 0.02), complication (p = 0.027), and mortality (p = 0.017). Conclusion: There was a significant correlation between lactic acid, NLR dan MLR with the outcomes of CAD patients (ICU LoS, complication and mortality) that have undergone CABG procedure.
Development of rabies eradication package, a family-based health promotion media to prevent rabies in Buleleng, Bali, Indonesia
Authors: Setiawan Komang Hendra , Probandari Ari Natalia , Pamungkasari Eti Poncorini , Tamtono Didik Gunawan 
Published: April 2020

Background : Rabies occurred in Bali since 2008, and in 2017 rabies cases were still found in Bali, including in Buleleng Regency. Prevention of rabies in Bali must involve the participation of the community especially family, which is in direct contact with dogs. This study aims to develop family-based health promotion media, which is later called as the rabies eradication package, to overcome rabies in Buleleng, Bali.Methods:Â This study uses the research and development (R&D) method. which consists of 5 phases, namely analysis, design, development, implementation and evaluation. at the implementation and evaluation stage researchers used a quasi-experimental approach. The total number of samples used was 100 families taken from 2 villages. The variables measured were the level of knowledge and attitudes towards prevention of rabies. data analysis using the Mann-Whitney U test.Result : Â Â This study develop rabies eradication package as a family-based health promotion media. This package consists of a rabies eradication book, a dog vaccination card, a medical history cards and bite wound treatment tools. Of the 100 respondents in the study sample, 50 respondents who were included in the treatment group, which received a rabies eradication package for 1 month, experienced a significant increase in knowledge (p <0.001) and attitudes (p <0.001) in prevention of rabies compared to the control group.Conclusion : Rabies eradication package can be used as a family-based health promotion media to increase rabies prevention efforts because it can increase knowledge and attitudes in preventing rabies
Developing Indonesian version of childbirth self-efficacy inventory (Cbsei)-C32
Authors: Kartini Farida , Emilia Ova , Dasuki Djaswadi , Prabandari Yayi Suryo 
Published: April 2020

Introduction: Women readiness and self-efficacy for pregnancy and childbirth is an important issue which usually assessed by using CBSEI-32 but currently no evaluation is ever conducted nor Indonesian version of this instrument ever developed to enhance subject understanding. Therefore, this study aimed to develop the Indonesian Language version of CBSEI (childbirth self-efficacy inventory) instrument.Method: A quantitative study was conducted by involving 73 pregnant mothers in their trimester III either as the primigravida or as multigravida. The selected respondents are those who lived in the working area or visited several health care centres in the area between July-August 2018. The content validity was tested by using Aiken V analysis and the construct validity was assessed using Kaiser-Meyer-Olkin (KMO) and Bartlettâ€™s test. Finally, the reliability test was conducted using Alpha Cronbach.Result: Â Analysis revealed that the Aiken V value of the instrument was in the range of 0.67-1 for the outcome expectancy and 0.75-1 for efficacy expectancy. 16 items in outcome expectancy showed KMO value > 0.5 with MSA value between 0.648-0.957.Â Meanwhile, 16 questions in efficacy expectancy also had KMO value > 0.5 with a MSA range between 0.798-0.937 respectively. The average values of the instrument validity for the outcome expectancy and efficacy expectancy were 0.740 and 0.726 respectively. Finally, Alpha Cronbach value for both outcome expectancy and efficacy expectancy was 0.94.Conclusion: According to the analysis, it can be concluded that CBSEI-C32 in Indonesia language version was valid and reliable instrument to be applied in pregnant mother in Indonesia.Â
Identification factors affecting adolescentâ€™s reproductive health behavior: a qualitative study
Authors: Triyanto Endang , Prabandari Yayi Suryo , Yuniarti Kwartarini Wahyu , Werdati Sri 
Published: April 2020

Introduction: Sexual risk behavior is mostly conducted by adolescents when the puberty started.Â The motivations underlies such behavior are varied including those related to individual, peers, family, and the environment. Therefore, this study aimed toÂ exploreÂ the factors that affect adolescentâ€™s reproductive health behavior.Methods: This study used qualitative method with purposive sampling on high risk and low risk adolescents.Â ExplorationÂ was conducted in accordance with several behavior theories such as Ecological Model of Health Behavior, Problem Behavior TheoryÂ and Integrated Behavior Model.Â Data collection was conducted by in-depth interviewsÂ in 2018 at Banyumas District.Â The results of the interviews wereÂ then analyzed by content analysisÂ technique.Â Results: According to the results ofÂ content analysis, weÂ found that reproductive behavior among adolescents was affected by two broad categories of factors namely individual internal factors and external factors.Â Individual internal factors were including age related risk, self-helplessness, knowledge, attitude, perceived norm, and lifestyle.Â External factors were determined by negative influence of peers, family, and environment risk. Peers, family, lifestyle and external factors seemed to influence personal values which could act as risk factor or protective factor depending on the nature of those factors involved. Conclusion: This research revealed that reproductive health behavior among adolescents was influenced by multiple factors that interact with one another.Â
Correlation between Loss of Cervical Lordosis and Degenerative Diseases of the Sub-axial Cervical Spine Columns
Authors: Martadiani Elysanti Dwi , Asih Made Widhi , Laksminigsih Nyoman Srie , Tjan Anastasia , Widyasari Nyoman 
Published: April 2020

Background: Subaxial cervical spine is the cervical segment which commonly undergo degenerative changes. There are numerous cervical lordosis angle measurement, however posterior tangential method is considered more accurate to detect any degenerative related changes.Aim: This study aimed to find the profile of cervical lordotic curve among patients with cervical degenerative disease, and to determine the correlation between degenerative process of subaxial cervical spine columns and cervical curve malalignment.Method: A cross sectional study was conducted by adopting the posterior tangent measurement. Cervical alignment was measured from cervical MRI mid sagittal section to identify loss of cervical lordotic and the presence of kyphosis. Degenerative disease of each vertebral column of subaxial cervical spine, anterior and posterior were evaluated. Spearman correlation test was done to identify the relationship between degenerative process of each column of subaxial cervical spine with loss of cervical lordosis and the presence of kyphosis. Multivariate analysis was done to adjust the effect of confounding variable.Result: Total of 90 subjects with male predominance (62%), mean age 54Â± 1.3 years. Neck pain contributes the most presenting complain (92%). As many as 51 subjects have hypo-lordosis, while 32 subjects have kyphotic changes. There was no significant correlation between degenerative process of each column of subaxial cervical spine and loss of cervical lordotic, but significant correlation was found between posterior listhesis and cervical kyphosis defomity. (p < 0.05).Conclusion: There was significant correlation between cervical kyphotic malalignment with posterior listheis Â in all three vertebra columns instead of hypo-lordosis.