bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative proteome analysis of the spinal dural arteriovenous fistula arterial draining vein with label-free quantitative proteomics
Authors: Liu Peixi , Shi Yuan , Li Sichen , Liu Yingjun , Zhou Yingjie , Song Yaying , Zhu Wei , An Qingzhu 
Published: January 2020

Abstract
Background: Spinal dural arteriovenous fistula (SDAVF) is the most common spinal vascular shunt lesion. Although pathological changes in the SDAVF draining vein (SDAVF-DV) have been elucidated, protein changes remain enigmatic. We investigated protein changes in the SDAVF-DV.Methods: Three SDAVF-DV samples were collected, and superficial temporal artery (STA) and superficial temporal vein (STV) samples were used as controls. After quantification and enzymolysis of the proteins, label-free quantitative proteomics was performed, and the peptide mixture was fractionated and analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify the differentially expressed proteins. Bioinformatics analysis of the differentially expressed proteins was also performed using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction (PPI) network analyses.Results: Compared with the STA, the SDAVF-DV had 195 upregulated proteins and 303 downregulated proteins. GO analysis showed that the most differential GO terms in each category were the adenylate cyclase-modulating G protein-coupled receptor signalling pathway, U6 snRNP and SH3 domain binding. KEGG pathway analysis showed that the most differentially expressed protein pathway was focal adhesion. Compared with the STV, the SDAVF-DV had 158 upregulated proteins and 362 downregulated proteins. GO analysis showed that the most differential GO terms in each category were lamellipodium assembly, U6 snRNP, and SH3 domain binding. KEGG pathway analysis showed that the most differentially expressed protein pathway was dilated cardiomyopathy. The PPI analysis revealed PPIs among the top 300 proteins.Conclusions: We demonstrated that the SDAVF-DV showed specific protein expression changes under long-period venous hypertension. The results of the present study will provide insights into the pathogenesis of SDAVF formation at the protein level. The proteomic results provide a scientific foundation for further study to explore the pathophysiological mechanism of SDAVF.
Fluorescent markers rhodamine B and uranine for Anopheles gambiae adults and matings
Authors: Aviles Erica i , Rotenberry Rachel D , Collins C Matilda , Dotson Ellen M , Benedict Mark Q 
Published: January 2020

Abstract
Background: Marking mosquitoes is vital for mark-release-recapture and many laboratory studies, but their small size precludes the use of methods that are available for larger animals such as unique identifier tags and radio devices. Fluorescent dust is the most commonly used method to distinguish released individuals from the wild population. Numerous colors and combinations can be used, however, dust sometimes affects longevity and behavior so alternatives that do not have these effects would contribute substantially. Rhodamine B has previously been demonstrated to be useful for marking adult Aedes aegypti males when added to the sugar meal. Unlike dust, this also marked the seminal fluid making it possible to detect matings by marked males in the spermatheca of females. Here we evaluate marking in Anopheles gambiae s.s. with rhodamine B and uranine to estimate their potential contribution. Methods:Two fluorescent markers, rhodamine B and uranine, were dissolved in sugar water and fed to adult Anopheles gambiae. Concentrations that are useful for marking individuals and seminal fluid were determined. The effects on adult longevity, the durability of the marking and detection of the marker in mated females was determined. We also evaluated effects on mating competitiveness.Results:Rhodamine B marking in adults is detectable for at least three weeks, however uranine marking declines with time and at low doses can be confused with auto-fluorescence. Both can be used for marking seminal fluid which can be detected in females mated by marked males, but, again, at low concentrations uranine-marking is more easily confused with the natural fluorescence of seminal fluid. Neither dye affected mating competitiveness.Conclusions:Both markers tested could be useful for field and laboratory studies. Their use has substantial potential to contribute to a greater understanding of the bio-ecology of this important malaria vector. Rhodamine B has the advantage that it appears to be permanent and is less easily confused with auto-fluorescence. The primary limitation of both methods is that sugar feeding is necessary for marking and adults must be held for at least 2 nights to ensure all individuals are marked whereas dusts provide immediate and thorough marking.
Trends in Male Contraceptive Use in Ghana: An Analysis of Self-Reported Contraceptive Use from GDHS 2003, 2008 &amp; 2014
Authors: Alando Cyril , Manu Adom , Habib Helen , Modey Emefa , Torpey Kwasi , Adanu Richard , Ankomah Augustine 
Published: January 2020

Abstract
Background

Male participation in contraception is proven to have positive outcomes on reproductive health and remains a critical issue of public health importance. However, there dearth of evidence on male contraceptive use is in Ghana. Knowledge of the trends and trajectories of male contraceptive use could inform programmatic interventions to boost male contraceptive use for effective fertility control. Thus, this study sought to examine the trends in male contraceptive use using the three most recent Demographic and Health Surveys conducted in Ghana in 2003, 2008 and 2014.
Methods

This paper examines trends in self-reported contraceptive use among sexually active Ghanaian males, using data from the three most recent national Demographic and Health Surveys (2003, 2008 and 2014). Frequency distributions of socio-demographic and background characteristics were used to describe the sample by each survey year. The Z test of proportions was used to identify significant differences in the estimated proportions and subgroups of male contraceptive use by survey year. The Combined data from the three nationally representative surveys were analysed, accruing a sample of 9,008, comprising (GDHS 2003=3,104; GDHS 2008=3,007; and GDHS 2014=2,897) male respondents who met the inclusion criteria.

Results

Male contraceptive use appears to have declined over the last three demographic surveys despite an increase in the proportion of sexually active males. A significant proportion of male contraception users (44.5% - 41.5%) still relied on the male condom as their primary contraception choice. Place of residence, educational attainment, occupation, region of residence and religious affiliation were all found to be significantly associated with male contraceptive use consistency.

Conclusions

There has been a significant decrease in contraceptive use by Ghanaian men between 2003 and 2014. The male condom appears to be the dominant modern male contraceptive method, especially during adolescence, but along the life course contraceptive use shifts towards modern female methods. There is a need for family planning service providers, policymakers and all relevant stakeholders to consciously target men with contraceptive products and services. Focusing programmes and policies towards improving men’s contraceptive use is an imperative for effective fertility regulation.

Key Words: Trend Analysis, Male Contraception, Family Planning, Ghana
Morphological Changes of Salicylic Acid Application on Pepper (Capsicum annuum L.) Seedling under Cold Condition
Authors: AHMED AHMED Mohammed , KARİPÇİN Muhemet Zeki , YAŞAR Fikret 
Published: January 2020

This
project was developed to investigate the contribution of salicylic acid (SA) to
the development of pepper seedlings grown in low temperature (0 0C)
conditions. The research was carried out in the controlled plant growing
cabinet in the research- investigation area of the Department of Horticulture,
Faculty of Agriculture, Siirt University. As a vegetable material, Urartu F1
pepper type (capia) which is used in greenhouse cultivation has been used. As a
dose of different salicylic acid; 0.01 and 0.05 mmol doses were applied. The
dose of 0 mmol salicylic acid was used as a control group. Application
frequency; It was applied 1 time, 2 times and 3 times.3 different cold
application times were also investigated; 24 hours, 48 hours and 72 hours. The
experiment was designed in randomized plots and 3 replications. In the pepper seedlings Rate of Lost Seedling Weight (ROLSW) and Rate of Lost Seedling Length (ROLSL) were investigated. At
the end of the research; Both SA applications increased the ROLSW rate
according to the control. The application of 0.01 ppm was the SA application
with the highest ROLSW rate. The application of 0.01 ppm SA also increased the
ROLSL rate compared to the control. The highest ROLSW and ROLSL rates were
obtained from 24-hour cold application. There was no statistically significant
difference between the frequencies of application.
Characterization of Secondary Metabolites in Two Cousinia species
Authors: PAŞAYEVA Leyla , FATULLAYEV Hanifa , KARAKUŞ İrem Nur , SOYLU Serap , TUGAY Osman 
Published: January 2020

Cousinia is one of the widespread genera of Asteraceae family. According to previous studies on some Cousinia species it was found that these species are rich of triterpenes, sesquiterpenes, flavonoids, acetylenes and steroids. According to our knowledge, there are no published reports on the chemical composition of C. iconica Hub. - Mor. and C. aintabensis Boiss. &amp; Hausskn., thus we aimed to investigate secondary metabolites of these species. In this study, the phytochemical constituents of these species were evaluated. Seven of identified compounds were quantified. The quantitative and qualitative determination of compounds within the extracts was carried out by LC-MS/MS. Phytochemical analyses revealed the presence of flavonoids, saponins, terpenes and steroids. Preliminary examination of the mass spectrums revealed the presence of phenolic acids and derivatives and flavonoid compounds in extracts. According to quantitative analyses the main compound of C. iconica (CI) and C. aintabensis (CA) extracts was rutin with the highest contents (169.779 µg/mgextract and 161.638 µg/mgextract). Moreover, qualitative and quantitative study combined with different biological activities will shed new lights to the advanced studies.
Acceptance and Commitment Therapy (ACT) to Improve Educators Self-Acceptance of Children with Special Needs
Authors: Barida Muya , Widyastuti Dian Ari 
Published: January 2020

Children with Special Needs are children with disabilities in the development of life due to disruption (mental, intellectual, emotional, social, physical) in the social-social, career, and academic fields, so they need special services and are different from children in general. This study aims to determine the effectiveness of Acceptance and Commitment Therapy (ACT) to increase the self-acceptance of educators towards Children with Special Needs. This study uses an experimental approach to the design of one group pretest-posttest. The study was conducted on teachers who are members of the HIMPAUDI Sleman District, who were randomly selected and obtained as many as 27 teachers. Data analysis was performed using the T-Test technique. Based on the results of the study, it appears that the self-acceptance of educators towards Children with Special Needs before and after the training on Acceptance and Commitment Therapy (ACT) increases, but the increase is less significant. Conclusion The Acceptance and Commitment Therapy (ACT) program is less effective in improving the self-acceptance of educators towards Children with Special Needs. Based on these results, it needs to be followed up with direct assistance or assistance in schools for educators who systematically help Children with Special Needs.
Managing Democracy in Malaysia (Identity, Minorities, and Representation)
Authors: Dwijayanto Arik , Afif Yusmicha Ulya , Fathoni Khoirul 
Published: January 2020

The Malaysian General Election (GE-14) in May 2018 raised an interesting and new phenomenon. For the first time since independence of Malaysia in 1957, the rulling alliance known as the National Front (Barisan Nasional, BN) which led by UMNO failed to secure simple majority in parliament and lost control of seven of Malaysia’s 13 states. This was due to the challenge presented by the new opposition alliance known as Pakatan Harapan (Alliance of Hope, PH) which won 113 of seats in parliament and gain control the central government. It is interesting that this significant increase in support for the opposition is due to their change the way minorities, identity and representation is managed. The Malaysian Supremacy as political manifesto of Pakatan Harapan which involves eradicating affirmative action based on etnicity, basing it instead on need, for instance need due to prosperity through the removal of government service tax (GST), and reformation of law. This would potentially increase the justice and equality for all ethnic or racial groups. This paper links the 2013 and 2018 elections, the more global trend in which minorities and youth representation are standing up to demand their right and to the challenge multiracial presents in Malaysia as a Muslim country. Therefore, the fundamental questions in this paper of how Pakatan Harapan manage minorities, identity and representation within in the process of democracy and to what extent the opportunities and the involvement of youth as voters and parliamentary candidates in order to support a progressive democracy in Malaysia.
Use of the Sentinel-2A Multispectral Image for Litho-Structural and Alteration Mapping in Al Glo’a Map Sheet (1/50,000) (Bou Azzer–El Graara Inlier, Central Anti-Atlas, Morocco)
Authors: El Atillah Abdessamad , El Morjani Zine El Abidine , Souhassou Mustapha 
Published: January 2020

Abstract
The discovery of natural resources remains the main mission of Earth observation satellites, especially in geographical areas that have a very difficult accessibility as those of the Bou Azzer–El Graara inlier (Central Anti-Atlas, Morocco). This work investigates the use of different satellite data, such as Sentinel-2A’s multispectral imagery, in order to direct the prospection program in an efficient manner, saving both time and cost. The image processing methods of Landsat 7, 8, and “Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER)” (30 m/15 m) were used to create methods for Sentinel-2A images (10 m). The red, green, blue (RGB) image 12.8.2, 11/12.11/2.11/8, principal component (PC) 1,2,3(11.12.2), and other new images were the result of principal component analysis (PCA), and classification by the Iterative Self-Organizing Data Analysis Technique (ISODATA) and K-Means allowed realization of a lithological cartography as well as maps of lineaments through directional filters and the ratio of 11/12 for hydrothermal alteration zone mapping. The assembly of lithological, structural, and hydrothermal alteration data gave an idea of the mineralogy of the study area. Validity of the results was tested by comparison with the field data and the geological maps of the studied site (62% for the hydrothermal alteration zone, 81% for the lithological map, and 74% for the structural map).
Kinematic PPP Using Mixed GPS/Glonass Single-Frequency Observations
Authors: Hamed Mostafa , Abdallah Ashraf , Farah Ashraf 
Published: January 2020

Abstract
Nowadays, Precise Point Positioning (PPP) is a very popular technique for Global Navigation Satellite System (GNSS) positioning. The advantage of PPP is its low cost as well as no distance limitation when compared with the differential technique. Single-frequency receivers have the advantage of cost effectiveness when compared with the expensive dual-frequency receivers, but the ionosphere error makes a difficulty to be completely mitigated. This research aims to assess the effect of using observations from both GPS and GLONASS constellations in comparison with GPS only for kinematic purposes using single-frequency observations. Six days of the year 2018 with single-frequency data for the Ethiopian IGS station named “ADIS” were processed epoch by epoch for 24 hours once with GPS-only observations and another with GPS/GLONASS observations. In addition to “ADIS” station, a kinematic track in the New Aswan City, Aswan, Egypt, has been observed using Leica GS15, geodetic type, dual-frequency, GPS/GLONASS GNSS receiver and single-frequency data have been processed. Net_Diff software was used for processing all the data. The results have been compared with a reference solution. Adding GLONASS satellites significantly improved the satellite number and Position Dilution Of Precision (PDOP) value and accordingly improved the accuracy of positioning. In the case of “ADIS” data, the 3D Root Mean Square Error (RMSE) ranged between 0.273 and 0.816 m for GPS only and improved to a range from 0.256 to 0.550 m for GPS/GLONASS for the 6 processed days. An average improvement ratio of 24%, 29%, 30%, and 29% in the east, north, height, and 3D position components, respectively, was achieved. For the kinematic trajectory, the 3D position RMSE improved from 0.733 m for GPS only to 0.638 m for GPS/GLONASS. The improvement ratios were 7%, 5%, 28%, and 13% in the east, north, height, and 3D position components, respectively, for the kinematic trajectory data. This opens the way to add observations from the other two constellations (Galileo and BeiDou) for more accuracy in future research.
Nutritional Evaluation of Fermented, Germinated and Roasted Pumpkin (Cucurbita maxima) Seed Flour
Authors: Akintade Adeyanmola Oluwaseyi , Awolu Olugbenga Olufemi , Ifesan Beatrice Olawumi 
Published: January 2020

Abstract
Freshly harvested pumpkin seed was processed into flour namely; raw (RPSF), fermented (FPSF), germinated (GPSF) and roasted (ROPSF). Various pumpkin seed flour obtained were investigated for nutritional properties using standard methods. Protein was higher in the fermented flour (35.85%) while the roasted flour (46.31%) possessed the highest fat content. Results obtained from processed pumpkin seed flour further revealed that glutamic acid (10.45-13.02 g/100g) and linoleic acid (134.72-419.70 µg/ml) were the most abundant amino acid and fatty acid, respectively. Processed pumpkin seed flour contained appreciable amount of minerals and demonstrated very good functional properties. It can be concluded that subjecting pumpkin seed to various processing methods enhanced its nutritional properties and may find application in food industry.
Information and Communication Infrastructures and New Business Models in Rural Areas: The Case of Molise Region in Italy
Authors: Ievoli Corrado , Belliggiano Angelo , Marandola Danilo , Milone Pierluigi , Ventura Flaminia 
Published: January 2020

Abstract
The paper deals with the role of ICT and the related infrastructures to induce innovations for sustainable rural development. In particular, it focuses on the innovations induced by ICT in farms and in new rural firms, and on how digital infrastructures support and generate social innovation mechanisms, leading to the consolidation of entrepreneurship and dissemination of ICT-based innovation in rural areas. The hypothesis is that the presence of digital infrastructures generates a double effect: overcoming the concept of geographical proximity (relevant for remote rural areas) and promoting social innovation. In particular, this paper examines the role of social innovation to create a new demand for products, services and organisational models for farms and rural enterprises, promoting further innovation. To target the objectives, the work analyses three case studies of new business models (BMs) based on ICT innovation. The analysis focuses on the most important interactions, learning and organisational processes within the new enterprises and among the new farms/enterprises and the other economic and institutional actors, and on how they were shaped and changed by the use of ICT, relating them to a conceptual model. These three cases, although pioneering, are important since they give an original response to some of the main problems and needs of remote and inner rural areas, as for the access to high value segment of food market, the information deliveries about attractiveness of landscape and countryside for foreigners, investors and tourists and the creation of new stable relation with consumers/citizens in the urban areas. The three cases have been analysed with the aim to identify how the ICT, and the related innovations, create an interconnection between four characteristic elements of the BMs (value creation, supply chain, customer interfaces, financial model) and the restructuring of proximity dimensions (cognitive, institutional, social, geographical, organizational). The work shows how these three cases have several communalities, but also different aspects with respect to our objective of analysis: there are different ways in which the four characteristic elements of the BM are constructed and also different in the role that the different dimensions of proximity play in structuring the innovation process in each one of them. More generally, the results of the work also lead to consider a new role for public investments in ICT infrastructures: public administrations should intervene in order to create a coherence within projects of public and private initiatives.
Coastal Countryside Innovation Dynamics in North-Western Russia
Authors: Mikhaylov Andrey S. , Mikhaylova Anna A. , Lachininskii Stanislav S. , Hvaley Dmitry V. 
Published: January 2020

Abstract
Coastal regions are generally conceived as highly advanced in terms of socioeconomic and innovative development. Acting as international contact zones, coastal agglomerations are described as gateways for absorbing new knowledge, technologies, business cultures, etc. Yet, this perception is based on studies of large coastal cities and agglomerations. In our study, we focus on coastalization effects manifested in rural settlements and evaluate the innovation capability of the economies of coastal rural areas. The research scope covers 13 municipalities of the Leningrad region, including 134 rural settlements. The research methodology is structured into three main blocks: the evaluation of the human capital, assessment of the favorability of the entrepreneurial environment, and analysis of susceptibility of local economies to innovations. The list of analyzed innovation dynamics parameters includes the geospatial data for the distribution of population, companies and individual entrepreneurs, localization of specialized support and innovation infrastructure, sectoral analysis of the economic structure, digitalization aspects, et cetera. The data coverage period is 2010–2019 with variations depending on the availability of individual indicators. The research findings reveal particular features of the countryside as compared to urban settlements. Strong asymmetries are observed between the development of rural settlements cross-influenced by coastalization, near-metropolitan location, and national border proximity.