bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide analysis of the AREB/ABF gene lineage in land plants and functional analysis of TaABF3 in Arabidopsis
Authors: Li Fangfang , Mei Fangming , Zhang Yifang , Li Shumin , Mao Hude 
Published: March 2020

Abstract
Background: Previous studies have shown that ABFs (abscisic acid-responsive transcription factors) are important ABA-signaling components that participate in abiotic stress response. However, little is known about the function of ABFs in Triticum aestivum. In addition, although various ABFs have been identified in other species, the phylogenetic relationship between ABF transcription factors has not been systemically investigated in land plants. Results: In this study, we systemically collected ABFs from land plants and analyzed the phylogenetic relationship of these ABF genes. The ABF genes are present in all the land plants we investigated, including moss, lycophyte, monocots, and eudicots. Furthermore, these ABF genes are phylogenetically divided into seven subgroups, differentiations that are supported by variation in the gene structure, protein properties, and motif patterns. We further demonstrated that the expression of ABF genes varies among different tissues and developmental stages, and are induced by one or more environmental stresses. Furthermore, we found that three wheat ABFs (TaABF1, TaABF2, and TaABF3) were significantly induced by drought stress. Compared with wild-type (WT) plants, transgenic Arabidopsis plants overexpressing TaABF3 displayed enhanced drought tolerance. Conclusions: These results provide important ground work for understanding the phylogenetic relationships between plant ABF genes. Our results also indicate that TaABFs may participate in regulating plant response to abiotic stresses.
High resolution computed tomography for the diagnosis of 2019 novel coronavirus (2019-nCoV) pneumonia: a study from multiple medical centers in western China
Authors: Li Hong-Wei , Zhuo Li-Hua , Yan Gao-Wu , Wang Ji-Sheng , Huang Guo-Ping , Li Jia-Bing , Fang-Zhang  , Long Yong-Jun , Deng Li-Hua , Su Li-Ping , Yao Hong-Chao , Das Sushant-Kumar , Li Yong , Yang Han-Feng , Du Yong 
Published: March 2020

Abstract
Objective: To evaluate the role of high-resolution computed tomography (HRCT) in the diagnosis of 2019 novel coronavirus (2019-nCoV) pneumonia and to provide experience in the early detection and diagnosis of 2019-nCoV pneumonia. Methods: 72 patients confirmed to be infected with 2019-nCoV from multiple medical centers in western China were retrospectively analyzed, including epidemiologic characteristics, clinical manifestations, laboratory findings and HRCT chest features. Results: All patients had lung parenchymal abnormalities on HRCT scans, which were mostly multifocal in both lungs and asymmetric in all patients, and were mostly in the peripheral or subpleural lung regions in 52 patients (72.22%), in the central lung regions in sixteen (22.22%), and in both lungs, with "white lung "manifestations in four (5.56%). Subpleural multifocal consolidation was predominant abnormality in 38 patients (52.78%). Ground-glass opacity was seen in 34 patients (47.22%). Interlobular septal thickening was found in 18patients, of which eight had only generally mild thickening with no zonal predominance. Reticulation was seen in 8 patients (11.11%), in all of whom it was mild and randomly distributed. In addition, both lungs of 28 patients had two or three CT imaging features. Out of these 72 patients, 36 were diagnosed as early stage, 32 patients as progressive stage and 4 patient as severe stage pneumonia. Moreover, the diagnostic accuracy of HRCT features combined with epidemiological history was not significantly different from the detection of viral nucleic acid (all P >0.05). Conclusion: The HRCT features of 2019-nCoV pneumonia are characteristic to a certain degree, which when combined with epidemiological history yield high clinical value in the early detection and diagnosis of 2019-nCoV pneumonia.Authors Hong-Wei Li, Li-Hua Zhuo, Gao-Wu Yan contributed equally to this work.
Epidemiological Characteristics of 417 patients infected with COVID-19 and 368 discharged cases among them in Shenzhen City, China
Authors: Yuan Bo , An Ya-Wen , Chen Yong-Xin , Yang Jing , Wang Jian-Chun , Li Wei-Xin , Wang Cheng , Song Shuo , Liu Han-Qing 
Published: March 2020

Abstract
The outbreak of Coronavirus Disease 2019 (COVID-19) is getting worse every day all over the world. The present study aimed to review the epidemiological characteristics of patients infected with COVID-19 in Shenzhen city, a super megacity of China, to provide some references for fighting to the coronavirus. We collected data of 417 patients with laboratory-confirmed COVID-19 of Shenzhen through March 7th, 2020. The epidemiological characteristics of the patients were analyzed. Besides, we collected the governmental measures of Shenzhen city, and the the dynamic changes of the epidemic outbreak. Governmental strategies such as early detection, early hospitalization and popular science etc. are effective for the prevention and control of the epidemic. Nearly 80% confirmed patients with COVID-19 in Shenzhen were in normal or mild conditions, and the mortality was less than 1%. Age, gender, exposure to source of transmission within 14 days and basic diseases are major risk factors for severe patients. Patients in characters of elder, male, with exposure of Wuhan and carried basic diseases had higher risk to be in severe condition (P<0.01; P<0.001). There were 368 patients discharged from hospital by the end of March 7th, 2020. Patients in severe conditions took more time from onset to discharge (P<0.001), so as those elder one (P<0.001) or who carried basic diseases (P<0.01). The differences of epidemiological characteristics between children and the elderly are still not clear. The nucleic acid test of some discharged patients returned to positive again, and the potential mechanisms need to be further explored. In conclusion, timely administrative intervention is necessary for prevention and control of the COVID-19 outbreak. Patients in characters of elder, male and carried basic diseases worthy more attention. The management of discharged patients, especially the investigation of the recurrence of positive SARS-CoV-2 RNA will be the focus on for the next step. Authors Bo Yuan and Ya-Wen An contributed equally to this work.
Prior vaccination with the rVSV-ZEBOV vaccine does not interfere with but improves the efficacy of postexposure antibody treatment in nonhuman primates exposed to Ebola virus
Authors: Cross Robert W. , Bornholdt Zachary A. , Prasad Abhishek N. , Geisbert Joan B. , Borisevich Viktoriya , Agans Krystle N. , Deer Daniel J. , Melody Kevin , Fenton Karla A. , Feldmann Heinz , Sprecher Armand , Zeitlin Larry , Geisbert Thomas W. 
Published: March 2020

Abstract
A replication-competent, vesicular stomatitis virus vaccine expressing the Ebola virus (EBOV) glycoprotein (GP) (rVSV-ZEBOV) was successfully used during the 2013-16 EBOV epidemic1. Additionally, chimeric and human monoclonal antibodies (mAb) against the EBOV GP showed promise in animals and EBOV patients when administered therapeutically2-6. Given the large number of at-risk humans being prophylactically vaccinated with rVSV-ZEBOV there is uncertainty regarding whether vaccination would preclude use of antibody treatments in the event of a known exposure of a recent vaccinee. To model a worst-case scenario, we performed a study using rhesus monkeys vaccinated or unvaccinated with the rVSV-ZEBOV vaccine. One day after vaccination, animals were challenged with a uniformly lethal dose of EBOV. Five vaccinated animals and five unvaccinated animals were then treated with the anti-EBOV GP mAb-based therapeutic MIL77 starting 3 days postexposure. Additionally, five vaccinated macaques received no therapeutic intervention. All five macaques that were vaccinated and subsequently treated with MIL77 showed no evidence of clinical illness and survived challenge. In contrast, all five animals that only received the rVSV-ZEBOV vaccine became ill and 2/5 survived; all five macaques that only received MIL77 only also became ill and 4/5 survived. Enhanced efficacy of vaccinated animals that were treated with MIL77 was associated with delayed EBOV viremia attributed to the vaccine. These results suggest that rVSV-ZEBOV augments immunotherapy.Authors Robert W. Cross, Zachary A. Bornholdt, and Abhishek N. Prasad contributed equally to this work.
A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats
Authors: Petković Branka , Kesić Srđan , Ristić Slavica , Pavković Željko , Podgorac Jelena , Stojadinović Gordana , Pešić Vesna 
Published: March 2020

Background:
Psychotic states related to psychostimulant misuse in patients with hepatitis C virus
infection may complicate acceptance and reaction to antiviral treatment. This observation equally applies to the
widely used ribavirin therapy.

Objective:
We examined psychomotor and body weight gain responses to low ribavirin doses after cessation of
intermittent amphetamine treatment in adult rats to assess its role in neurobehavioral outcome during psychostimulant
withdrawal.

Method:
The model of amphetamine-induced (1.5 mg/kg/day, i.p., 7 consecutive days) motor sensitization and
affected body weight gain was established in adult male Wistar rats. Then, additional cohort of amphetaminesensitized
rats was subjected to saline (0.9% NaCl; 1 mL/kg/day; i.p.) or ribavirin (10, 20 and 30 mg/kg/day, i.p.)
treatment for 7 consecutive days. Animals’ motor activity in a novel environment was monitored after the 1st and
the 7th saline/ribavirin injection. Body weight gain was calculated as appropriate. Determination and quantification
of ribavirin in the brain tissue were performed also.

Results:
The 1st application of ribavirin to amphetamine-sensitized rats affected/decreased their novelty-induced
motor activity only at a dose of 30 mg/kg. After the 7th application, ribavirin 30 mg/kg/day still decreased, while
10 and 20 mg/kg/day increased novelty-induced motor activity. These behavioral effects coincided with the time
required to reach maximum ribavirin concentration in the brain. Body weight gain during withdrawal was not
influenced by any of the doses tested.

Conclusion:
Ribavirin displays central effects that in repeated treatment, depending on the applied dose, could
significantly influence psychomotor response but not body weight gain during psychostimulant/amphetamine
withdrawal.
Pd/C-catalyzed Synthesis of 4-biaryl Substituted 2-amino benzo[h] chromene Derivatives as Potential Cytotoxic Agents
Authors: Rani Chekuri Sharmila , Haritha Meda , Basaveswara Rao Mandava V. , Pal Manojit 
Published: March 2020

Background:
We report the design, synthesis and evaluation of a series of 4-biaryl
substituted 2-amino benzo[h]chromene derivatives as potential cytotoxic agents.

Methods:
The one-pot synthesis of this class of compounds was carried out under
ultrasound irradiation using an MCR involving Pd/C-catalyzed Suzuki-Miyaura coupling as a
key step.

Result and Conclusion:
Several of these compounds showed promising cytotoxicities when tested
against cancer cell lines but no or little effects on non-cancerous cell lines.
High Throughput Screening and Molecular Docking of Calmodulin with Antagonists of Trifluoperazine and Phenothiazine Chemical Class
Authors: Messaoudi Abdelmonem , Basharat Zarrin , Naqvi Asif 
Published: March 2020

Background:
Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind
to and regulate a multitude of different protein targets, thereby affecting many different cellular
functions. Binding ofsmall, hydrophobic molecules alter its function, as in the case of Phenothiazine
(PTZ). PTZ isan organic compound with a history of use as an antihelminth and frequently
quoted as a classic example of a pharmaceutical lead structure.Its derivatives are currently used as
antipsychotic drugs. Triflouperazine (TFP) is a phenothiazine derivative and a dopamine antagonist,
with antiemetic and antipsychotic activities. TFP exerts its antipsychotic effect by blocking central
dopamine receptors, thereby preventing effects like delusions and hallucinations caused by an excess
of dopamine. This agent also functions as a calmodulin inhibitor, thereby leading to an elevation
of cytosolic calcium. We have attempted to screen the extent of CaM specificity towards different
classes of TFP and PTZ.

Methods:
Molecular docking approach using the Lamarckian Genetic Algorithm was used to elucidate
the basis of structural similarity of TFP and PTZ with CaM. In total, 3000 compounds were
studied. All of the antagonists were taken from PubChem database on the basis of the structural
similarity of TFP and PTZ.

Results:
The docking result of these molecules with CaM demonstrated favored binding energies in
the range of -11.50 kcal/mol to -4.51 kcal/mol, with 8 molecules showing hydrogen bonds with the
active site residue Met124. Compound 1 was found to be the best CaM inhibitor. The Drug like and
chemical toxicity was also predicted for this compound.

Conclusion:
Computational docking is a feasible method to screen inhibitor compounds against a
biomolecule of interest.
The Effect of Feeding Date Palm Fruit (Phoeix dactylifera L.) on Energy, Macronutrient, and Fiber Intakes of a Convenient Sample of Females
Authors: Al-Sayyed Hiba F. , Takruri Hamed R. , Bakir Nawal A. , Takruri Dima H. 
Published: March 2020

Background:
Female menstrual changes in hormone concentration influence appetite and
eating behavior. As well, eating pattern has some influences on menstrual hormonal status. Feeding
date palm fruit has been found in our previous report to affect menstrual hormones and some menstrual
parameters.

Objective:
The aim of this research is to study the effect of feeding dates on energy, macronutrient,
and fiber intakes, body weight, and body fat%. Additionally, this study aims to find any correlation
between menstrual hormone concentration and the above-mentioned parameters.

Methods:
A convenient sample (n=37) of not-sexually active females aged 20-30 were divided into
two groups; one group (dates group) was fed 7 dates and the other served as a control group. The participants
of the control group were exposed to the same experimental conditions except for eating
dates. Throughout the feeding trial, the volunteers filled a 3-day food record; one menses day and
two non-menses days.

Results:
There was a significant effect of the menstrual status-feeding duration interaction in terms
of protein and fat intakes. Additionally, menstrual status, as well as feeding duration, affected energy
and macronutrient intakes significantly. Furthermore, feeding duration affected fiber intake significantly.
Despite the above-mentioned differences in energy and macronutrient intakes, these effects
didn’t affect the body weight and body mass index of the study participants, neither their body fat%.
Moreover, we have found significant correlations between nutrient intakes and menstrual hormone
concentration.

Conclusion:
Feeding dates affected energy, macronutrient, and fiber intakes significantly. These effects
were related to the changes in menstrual hormone concentration.
A magyarországi romák népességszámának prognózisa regionális szinten 2061-ig
Authors: Hablicsek Laszlo , Hablicsekne Richter Maria , Langi Tamas 
Published: March 2020

Tanulmányunkban megkísérelünk átfogó képet adni a magyarországi roma népesség országos és területi demográfiai jellemzőiről, előreszámítást készíteni akövetkező évtizedekre 2061-ig, régiók szerinti bontásban. A vizsgálat aktualitását jelzi, hogy az elmúlt évtizedekben a hazai roma népesség létszáma, illetve a népességen belüli aránya folyamatosan és jelentősen emelkedett. A következő évtizedekben mindkét téren további emelkedés várható, viszont a növekedés üteme lelassul. A roma népesség esetében a termékenység tovább csökken, a halálozás is mérséklődik, a várható élettartam emelkedik, ezek következményeként az idősek aránya egyre jobban nő. Becslésünk azt mutatja, hogy 2061-re a besorolás szerinti cigányság korfája átalakul, egyre inkább hasonlóvá válik a jelenlegi, teljes magyarországi népességéhez.
Journal of Economic Literature (JEL) kódok: C82, J11, J13, J19
Information About Sunscreen on YouTube and Considerations for Sun Safety Promotion: Content Analysis
Authors: Julian Anne K , Welch Jessica , Bean Maddison M , Shahid Sarah , Perna Frank M 
Published: March 2020

BackgroundSunscreen use is a popular sun protection method; however, application of sunscreen rarely meets the standards recommended for effectiveness. Access to information about how to effectively use sunscreen may play a role in proper sunscreen application. The internet is a common health information source; however, the quality of sunscreen-related content varies.ObjectiveThe objective of this study was to examine information about sunscreen in YouTube videos by video source.MethodsIn November 2017, the authors identified the 20 most popular YouTube videos (sorted by view count and relevance) for each of these 5 search terms: sunscreen cancer, sunscreen health, sunscreen information, sunscreen ingredients, and sunscreen natural. The inclusion criteria were English language and view count >1000 (N=111 unique videos). We double-coded videos for standard recommendations for sunscreen use (eg, apply 20 minutes before sun exposure), use of outdated terminology, and recommendation of complementary sun safety strategies.ResultsThe view counts ranged from 1100 to 671,142 (median 17,774, SD 109,651) and the average daily views ranged from 1 to 1448 (median 23, SD 234). End users (46/111, 41.4%) and health care providers (24/111, 21.6%) were the most common sources, and none of the most popular videos were produced by federal agencies or cancer-related nongovernmental organizations. Health care provider videos included marginally more recommendations than end user videos (mean 1.46, SD 1.96 vs mean 1.05, SD 1.20), but few (19/111, 17.1%) mentioned reapplication. The videos were generally positive toward sunscreen (82/111, 73.9%); however, some videos were negative (29/111, 26.1%), with warnings about the health risks of chemical sunscreens and their ingredients. Do-it-yourself sunscreen tutorials represented 19/111 (17.1%) of the sample.ConclusionsYouTube is a potential source for disseminating sun safety messages; however, the quality of its sunscreen content varies. Most of the videos in our study failed to include important sunscreen use recommendations. Clinicians should be prepared to address the information needs of patients by discussing effective, evidence-based sunscreen application and recommending a combined sun safety approach.
The Impact of a Social Networking Service–Enhanced Smart Care Model on Stage 5 Chronic Kidney Disease: Quasi-Experimental Study
Authors: Yang Feng-Jung , Hou Ying-Hui , Chang Ray-E 
Published: March 2020

Background
Stage 5 chronic kidney disease (CKD) presents a high risk for dialysis initiation and for complications such as uremic encephalopathy, uremic symptoms, gastrointestinal bleeding, and infection. One of the most common barriers to health care for patients with stage 5 CKD is poor continuity of care due to unresolved communication gaps.

Objective
Our aim was to establish a powerful care model that includes the use of a social networking service (SNS) to improve care quality for patients with CKD and safely delay dialysis initiation.

Methods
We used a retrospective cohort of CKD patients aged 20-85 years who received care between 2007 and 2017 to evaluate the efficacy of incorporating an SNS into the health care system. In 2014, author F-JY, a nephrologist at the National Taiwan University Hospital Yunlin Branch, started to use an SNS app to connect with stage 5 CKD patients and their families. In cases of emergency, patients and families could quickly report any condition to F-JY. Using this app, F-JY helped facilitate productive interactions between these patients and the health care system. The intention was to safely delay the initiation of dialysis therapy. We divided patients into four groups: group 1 (G1) included patients at the study hospital during the 2007-2014 period who had contact only with nephrologists other than F-JY; group 2 (G2) included patients who visited F-JY during the 2007-2014 period before he began using the SNS app; group 3 (G3) included patients who visited nephrologists other than F-JY during the 2014-2017 period and had no interactions using the SNS; and group 4 (G4) included patients who visited F-JY during the 2014-2017 period and interacted with him using the SNS app.

Results
We recruited 209 patients with stage 5 CKD who had been enrolled in the study hospital’s CKD program between 2007 and 2017. Each of the four groups initiated dialysis at different times. Before adjusting for baseline estimated glomerular filtration rate (eGFR), the G4 patients had a longer time to dialysis (mean 761.7 days, SD 616.2 days) than the other groups (G1: mean 403.6 days, SD 409.4 days, P=.011 for G4 vs G1; G2: 394.8 days, SD 318.8 days, P=.04; G3: 369.1 days, SD 330.8 days, P=.049). After adjusting for baseline eGFR, G4 had a longer duration for each eGFR drop (mean 84.8 days, SD 65.1 days) than the other groups (G1: mean 43.5 days, SD 45.4 days, P=.005; G2: mean 42.5 days, SD 26.5 days, P=.03; G3: mean 3.8.7 days, SD 33.5 days, P=.002).

Conclusions
The use of an SNS app between patients with stage 5 CKD and their physicians can reduce the communication gap between them and create benefits such as prolonging time-to-dialysis initiation. The role of SNSs and associated care models should be further investigated in a larger population.