bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and characterization of paclitaxel and embelin loaded solid lipid nanoparticles for breast cancer
Authors: Kumar Ajay , Kaur Veerpal , Singh Amandeep , Mishra Neeraj 
Published: January 2020

In an effort to develop an alternative formulation of combination of paclitaxel (PTX) and embelin (EMB) suitable for parenteral administration, PTX-EMB loaded sterically stabilized solid lipid nanoparticles (SLNs) were prepared, characterized and examined for in vitro cytotoxicity. The SLNs, comprising glycerol mono stearate (GMS) as a solid lipid core, Brij 35 used as surfactant and PEGylated phospholipid used as stabilizer, were prepared using a hot homogenization method. Optimized PTX-EMB loaded formulation, the particle sizes of the prepared SLNs were around 300 nm, suggesting that they would be suitable as a parenteral formulation. Transmission electron microscopy showed that the SLNs were homogeneous and spherical in shape. Entrapment efficiency of paclitaxel and embelin was 92.83 ± 2.2%, 83.25 ± 2.4% respectively. An in vitro drug release study were performed in PBS (pH 7.4) for 80 hrs and observed that paclitaxel and embelin released from the PEGylated SLNs was 93.91 ± 4.1 % and 75.63 ± 4.37 % respectively. Furthermore, treatment of the MCF-7 breast cancer cell line with PTX-EMB loaded SLNs yielded cytotoxicities comparable to PTX solution, PTX-EMB mixture solution and PTX loaded PEGylated SLNs. These results collectively suggest that our optimized SLN formulation may have a potential as alternative delivery system for parenteral administration of paclitaxel and embelin.
Keywords: Embelin, Apoptosis, Cancer, Cytotoxicity, Breast Cancer, Solid lipid nanoparticles.
The Benefit and Safety of Vitamin B Combination to Reduce Fatigue and Improve Quality of Life in Chronic Kidney Disease Patients
Authors: Pinzon Rizaldy Taslim , Sanyasi Rosa De Lima Renita , Pramudita Esdras Ardi 
Published: January 2020

Background: Chronic kidney disease (CKD) patients often have a chronic fatigue and lower QoL, thus may increase morbidity and mortality in CKD patients. Objective: The objective was to identify the benefit and safety of administration of intravenous vitamin B combination to reducing fatigue, measure the prevalence of fatigue and QoL in CKD patients. Method: This study was an observational non control study for 4 weeks in CKD patients with routine hemodyalisis 2 times/week. Every subject has a routine administration of intravenous vitamin B combination, consist of 100 mg vitamin B1, 100 mg vitamin B6, and 5000 mcg vitamin B12, after each hemodyalisis. Visual analogue scale (VAS) used to measure the degree of fatigue. QoL measured using SF-8 questionnaire, consist of total, physical (PCS), and mental component (MCS). Results: The prevalence of fatigue is high. The mean VAS score was 3.4±2.1 at baseline and there were 46.7% subjects with VAS score above the mean VAS score. After 4 weeks administration of vitamin B combination intravenously, the mean VAS score was decreasing from 3.4 to 2.7. The prevalence of fatigue was decreasing from 46.7% to 41%. This shifting was statistically significant (p:0.008). There was a score reduction in total component (20.19±4.8 to 19.29±4.9), PCS (13.44±3.1 to 12.99±3.5), and MCS (6.75±2.1 to 6.31±1.9). This score reduction indicated a better QoL. However, the reduction was not statistically significant. Conclusion: The prevalence of fatigue is high. Intravenous vitamin B combination considered to be effective and safe to reducing the degree of fatigue in CKD patients.
Keywords: chronic kidney disease, fatigue, hyperhomocysteinemia, vitamin B combination, quality of life
Analytical Method Development and Validation for the Estimation of Sugammadex
Authors: Mashru Rajashree , Parekh Hemangi , Chokshi Parin 
Published: January 2020

A simple, precise, accurate, specific RP-HPLC method developed for sugammadex in bulk and simulated mixture. Chromatographic separation is achieved by C18 column (250 x 4.6 mm, 5µ) in isocratic mode. The optimized mobile phase consists of acetonitrile and double distilled water in ratio of 20:80%v/v at a flow rate of 0.5mL/min and sugammadex was monitored at 210nm. Retention time of the drug was found to be 3.39min. The linearity obtained in range of 50 – 250 µg/mL. %RSD mean for precision and %Recovery mean of the sugammadex were found to be 0.63 and 99.04% - 99.84% respectively. Stability indicating nature of RP-HPLC method was established by applying the degradation condition. The results indicate that developed RP-HPLC method would be suitable for estimation of drug in presence of degradant product. The above developed method was validated according to ICH guideline.
Keywords: Sugammadex, Assay, Simulated Mixture, Forced Degradation, Validation.
Use of Molecular Diagnostic Techniques to Determinate the Epidemiology of Malaria Parasites in North Eastern Nigeria
Authors: Lawan M.M. , Shago And M.I. 
Published: January 2020

The emergence of resistance to all antimalarial drugs in clinical use is now making it necessary to discover the markers responsible for the resistance. The principal aim of this research is the use of molecular diagnostic techniques to Determine the epidemiology of malaria parasites. Thirty blood samples were analyzed by microscopy and molecular techniques to monitor the relative efficiency in malaria diagnosis. Molecular analysis revealed 28 out of 30 samples as positive for malaria while Microscopic analysis revealed 27out of 30 samples as positive malaria parasite. The molecular analysis was particularly useful to unveil parasites presence in infections not detectable by blood smear analysis.
Keywords: Molecular Diagnostic Techniques, Epidemiology, Malaria Parasites
Development and Validation of Stability Indicating RP-HPLC Method for Estimation of Cilnidipine
Authors: Tiwari Balakrishna , Shirsat Mrunal K. , Kulkarni Amol 
Published: January 2020

Cilnidipine is one of the dihydropyridine calcium antagonists. It was created combinedly by Fuji Viscera Pharmaceutical Company, Ajinomoto and Japan and was approved in the year 1995. Cilnidipine acts on N-type calcium channel where exist the end of sympathetic nerve in addition to common L-type calcium channel like that of other calcium antagonists. China, Japan, India, Korea and several other countries approved this drug. The objective of the method validation is to demonstrate whether the method was suited for the intended purpose. The method was validated as per the ICH guidelines. The method was validated for linearity, precision (repeatability, intermediate precision), accuracy, specificity, robustness, limit of detection and limit of quantification. Cosmosil (4.6 X 250mm, 5 μ) column was used for separation. The selected wavelength for Cilnidipine was 241 nm. The mobile phase consists Methanol: Potassium dihydrogen phosphate buffer (50:50). Flow rate was delivered at 1.0 mL/min. Appropriate dilutions of standard stock solutions were prepared as per the get desired concentrations in the range of 100-500 mcg/ml. The RT obtained was 4.8165 minutes.
Keywords: Cilnidipine, UV spectroscopy, RP-HPLC, ICH
Bisphenol-A in Wistar Rats: Toxicological Study as Mitochondrial Disrupting Agent
Authors: Zahak Ahmad , Saraswat Rohit 
Published: January 2020

BPA is an EDC which is continuously released into the environment because of its extensive usage as plasticizer and other industrial applications. The present study establishes BPA induced alterations in mitochondrial marker enzymes and hence cause mitochondrial dysfunction. Studies were performed to evaluate the histopathological changes in tissue on exposure to different doses of BPA for different time periods. Light microscopic examination of samples was done to evaluate the possible changes caused by treatment of different doses of BPA. After getting the results in light microscopy the changes and damage caused by BPA ultra-structurally were observed. Light microscopic study showed that BPA exposure for short duration of 7 days did not resulted in significant change in histology of Rat of treated animals. Light microscopic observations showed that low doses of BPA (5 and 10 mg/kg body weight) exposure for 14 days caused reduction in the number of cells in the layers of seminiferous tubules. Highest dose of BPA treatment for 14 days caused much damage to the seminiferous tubules. There were many ultra-structural changes found in the treated groups as compared to the control group. Hence it can be stated that due to the toxic effect of BPA the mitochondrial marker enzymes have shown marked reduction in their activities.
Keywords: BPA, EDCs, Light microscopic, Mitochondria and Toxicity.
Identifikasi Bawah Permukaan Di Daerah Panasbumi Menggunakan Geolistrik Di Desa Tolole Kecamatan Ampibabo Kabupaten Parigi Moutong
Authors: Fahri Moh. , Abdullah Abdullah , Rugayya Sitti , Badaruddin Badaruddin 
Published: January 2020

Telah dilakukan identifikasi bawah permukaan di daerah panasbumi menggunakan metode geolistrik di Desa Tolole Kecamatan Ampibabo Kabupaten Parigi Moutong. Penelitian ini bertujuan untuk mengetahui lapisan bawah permukaan. Pengukuran dilakukan sebanyak 4 lintasan dengan metode Automatic Array Scanning (AAS) konfigurasi Wenner-Schlumberger. Data hasil pengukuran diolah dengan menggunakan program Res2Dinv 3.53 dan Surfer 11. Hasil penelitian lapisan bawah permukaan di daerah panasbumi terdiri dari 3 lapisan. Lapisan pertama dengan nilai hambatan jenis 2 sampai 17,5 m yang diduga merupakan lempung pasiran, lapisan kedua dengan nilai hambatan jenis 17,5 sampai 104 m yang diduga merupakan pasir dan kerikil. Pada lapisan kedua ini terdapat air panas yang tersebar pada permukaan yang berbentuk manifestasi sampai dengan kedalaman ± 44 m bmt dengan faktor formasi 2 sampai 5 pada formasi aluvium dan endapan pantai. Sedangkan pada lapisan ketiga dengan nilai hambatan jenis 104 sampai 247 m yang diduga merupakan pasir, krikil dan lempung.
Kata kunci: Geolistrik, Panasbumi, Res2dinv, Surfer, Wenner-Schlumberger
PENGARUH VARIASI WAKTU DAN KECEPATAN PENGADUKAN DALAM PEMBUATAN ETIL ESTER DARI MINYAK KELAPA
Authors: Jusman Jusman , Setiaji Bambang , Triyono Triyono , Syoufian Akhmad 
Published: January 2020

Penelitian ini bertujuan memperoleh etil ester yang optimum. Penelitian ini diawali dengan reaksi transesterifikasi antara minyak kelapa dan etanol dengan variasi waktu dan kecepatan pengadukan. Etil ester dianalisis dengan GC dan GC-MS. Hasil transesterifikasi optimum sebesar 76,48 % diperoleh pada rasio minyak kelapa/etanol/NaOH 1:2:0,02 (100 mL : 50 mL : 2 g) dengan kecepatan pengadukan 300 rpm. Kecepatan pengadukan dan waktu reaksi berturut-turut di atas 400 rpm dan 30 menit menghasilkan rendamen etil ester di atas 80 %. Kata Kunci : minyak kelapa, transesterifikasi, variasi waktu, dan kecepatan pengadukan
ANALISIS KANDUNGAN PROTEIN TERLARUT DAN KAROTENOID NASI JAGUNG (Zea mays var. indentata) YANG DIFERMENTASI DENGAN KAPANG ONCOM MERA (Neurospora sp.) Analysis of Dissolved Protein and Carotenoid Content of Corn Rice (Zea mays var. indentata) Fermented with Red Oncom Shells (Neurospora sp.)
Authors: Nurfahriani Dwi , Nurhaeni Nurhaeni , Khairuddin Khairuddin 
Published: January 2020

Analisis kandungan protein terlarut dan karotenoid nasi jagung (zea mays var. indentata) yang difermentasi oleh kapang oncom merah (neurospora sp.). Penelitian ini bertujuan untuk mengetahui konsentrasi inokulum kapang oncom merah dan waktu fermentasi yang dapat menghasilkan nasi jagung dengan kadar protein terlarut dan karotenoid tertinggi. Penelitian menggunakan rancangan acak lengkap pola faktorial yang terdiri atas dua faktor yaitu : faktor a (konsentrasi inokulum kapang oncom merah) terdiri atas 3 taraf meliputi : 1%; 1,5% dan 2%. Faktor b (waktu inkubasi) terdiri atas 3 taraf : waktu fermentasi 36 jam; 48 jam dan 60 jam. Hasil analisis diperoleh perlakuan yang terbaik adalah konsentrasi 2% dan waktu fermentasi 48 jam dengan kadar protein terlarut 22,4% dan kadar karotenoid 0,38 mg. Analisis statistic sidik ragam menunjukkan bahwa tidak terdapat korelasi antara konsentrasi inokulum dan waktu fermentasi terhadap kadar protein terlarut dan kadar karotenoid.Kata kunci: nasi jagung, kapang oncom merah, protein terlarut, karotenoid.
PRODUKSI MALTODEKSTRIN DARI PATI UMBI TALAS (Colocasia esculenta) MENGGUNAKAN ENZIM α-AMILASE
Authors: Sao Febby Prisca Valentina , Bahri Syaiful , Indriani Indriani 
Published: January 2020

Telah dilakukan penelitian tentang produksi maltodekstrin dari pati umbi talas (Colocasia esculenta) menggunakan enzim alfa amilase. Tujuannya adalah untuk menghasilkan maltodekstrin dengan variasi berat pati terhadap enzim dan waktu hidrolisis terbaik yang diperlukan untuk memperoleh maltodekstrin dengan rendemen dan nilai DE ≤20. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 2 faktor yaitu berat pati dan waktu hidrolisis yang masing–masing dilakukan sebanyak 3 kali. Hasil penelitian menunjukan bahwa rendemen maltodekstrin tertinggi diperoleh pada berat pati terhadap enzim 25 g dengan nilai 54,93% dan DE 12,56. Waktu hidrolisis pati yang menghasilkan rendemen maltodekstrin tertinggi adalah pada waktu 180 menit yaitu 64,29% dengan nilai DE maltodekstrin yang diperoleh yaitu 11,37.Kata Kunci :Enzim α- amilase, maltodekstrin, pati umbi talas.
UJI STABILITAS PIGMEN EKSTRAK UBI BANGGAI (Dioscorea bulbifera var celebica Burkill) PADA BERBAGAI VARIASI pH DAN LAMA PAPARAN SINAR MATAHARI
Authors: Rundubelo Bryan Agung , Ridhay Ahmad , Hardi Jaya , Pusptasari Dwi Juli 
Published: January 2020

Penelitian tentang potensi ubi banggai (Dioscorea bulbifera var celebica Burkill) sebagai pewarna alami makanan dan minuman yang ditinjau dari stabilitas pH dan paparan sinar matahari telah dilaksanakan. Tujuan dari penelitian ini adalah mengetahui kestabilan ekstrak ubi banggai terhadap pengaruh pH dan terhadap paparan sinar matahari selama masa penyimpanan. Waktu yang digunakan adalah 10 hari untuk penyimpanan terhadap pengaruh pH dan 5 jam untuk paparan sinar matahari. Hasil penelitian menunjukan zat warna ekstrak ubi banggai stabil pada pH 2 setelah masa penyimpanan 10 hari dengan presentasi degradasi 37.38 % sementara pengaruh paparan sinar matahari menghasilkan degradasi terendah pada botol gelap yaitu 36,91 % dibandingkan dengan botol bening 41,96 %.Kata Kunci : Zat warna, stabilitas ubi banggai, degradasi
PEMBUATAN Carboxymethyl cellulose (CMC) DARI PELEPAH NANAS (Ananas cosmosus Merr.)
Authors: Ripdayana Ripdayana , Ridhay Ahmad , Rahim Erwin Abdul 
Published: January 2020

A research about production of Carboxymethyl cellulose (CMC) from pineapple midrib (Ananas cosmosus Merr.) has been conducted. This research aims to determine the best cellulose ratio with sodium monochloro acetate and the best reaction time to the substitution degree of carboxymethyl cellulose from pineapple midrib fibers. This study used a Completely Randomized Design method with two independent variables, i.e. variations in the weight of sodium monochloroacetate (2.5 grams, 3 grams, 3.5 grams, 4 grams, and 4.5 grams) and variations in reaction time (1 hour, 2 hours, 3 hours, 4 hours , and 5 hours), each treatment was done duplo using 20 treatment units. The result of the study showed that the best ratio of sodium monochloroacetate to cellulose which produced carboxymethyl cellulose from pineapple midribs was 2.5: 2.5 with substitution degree value of 1.93 and optimum reaction time which produced carboxymethyl cellulose from pineapple midribs was 1 hour with substitution degree of 2.38.
Keywords: Carboxymethyl cellulose, Pineapple midrib
STABILITAS EKSTRAK WARNA BUNGA ASOKA (Ixora javanica) BERDASARKAN VARIASI pH SELAMA MASA PENYIMPANAN
Authors: Jenianti Ellditna , Nurhaeni  , Satrimafitrah Pasjan , Pusptasari Dwi Juli 
Published: January 2020

Research on the potential of Ashoka (Ixora javanica) flower as a natural food and beverage coloring agent in terms of pH stability during the storage period. This study aims to determine the stability of Asoka flower extract on the effect of pH during the storage period. The time used was 10 days and the variation of pH of 2,3,4,5 and 6, respectively. The results show that Asoka flower extract was stable at pH 2 after a 10-day storage period with degradation presentation of 36.21%, while Ashoka flower extract dyes were unstable at pH 6 after a 10-day storage period with degradation percentage is 78.02%. This proves that the different acidic atmosphere influences the stability of Ashoka flower extract.
Keywords : Dyes, stability of Ashoka flowers, degradation
ANALISIS KANDUNGAN NUTRISI MIE KERING YANG DISUBTITUSIKAN AMPAS KELAPA
Authors: Bawias Sylvia Florensy , Syamsuddin  , Prismawiryanti  , Sumarni Ni Ketut 
Published: January 2020

Research has been carried out on the nutritional content of dried noodles substituted with coconut pulp. This study aims to determine the highest total protein content, highest crude fiber content and the highest water content and organoleptic quality of dried noodles substituted with coconut pulp. Dry noodles are made from wheat flour and coconut pulp as the main ingredients. The material ratio is varied to 90:10; 80:20; 70:30 and without the addition of coconut pulp flour. Dry noodles without the addition of coconut pulp flour produced the highest total protein content of 18.57%(b/b) and at the ratio of 70:30 the highest crude fiber was 17.55% (w/w) and the highest water content was 2.63% (w/w). Based on the quality of organoleptic dry noodles the most preferred is at the ratio of 90:10.
Keywords: Dry noodles, coconut pulp flour, total protein content, crude fiber content, water content, organoleptic quality
PENGARUH JENIS PELARUT TERHADAP EKSTRAKSI DARI KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)
Authors: Noviyanty Amalia , Salingkat Chitra Anggriani , Syamsiar Syamsiar 
Published: January 2020

This study aims to determine the effect of the type of solvent on extract yield, total phenolics and IC50 values of the skin of red dragon fruit and also obtain the best type of solvent that provides extract rendement, total phenolics and the highest or best IC50 value for extracting red dragon fruit peels. There are 3 types of solvents used (95% ethanol, ethyl acetate and acetone: water (7: 3) with a sample-solvent ratio (5:1 v/b). The data obtained were analyzed using a Completely Randomized Design that was applied to observations of extract rendemen, phenolics total and IC50 values, if the treatment had a very significant or significant effect followed by continued BNJ test at 1% or 5% level. The results showed that the solvent ratio very significantly affected the extract rendemen, phenolics total and IC50 value of red dragon fruit peel extract. Ethanol 95% solvent is the best solvent for extracting dragon fruit peels because it has the highest yield (26.15%), total phenolic (64.75 ppm) and antioxidant activity (IC50 value) (120.53 ppm) highest or best.
Keywords : IC50, the red dragon fruit skin, solvent ratio, extract yield, phenolics total
Düşey Düz bir Levhanın Periyodik Salınımlarının Isı Transferine Etkisi
Authors: AKÇAY Selma , AKDAĞ Ünal 
Published: January 2020

Bu çalışmada, düşey düz bir levhanın periyodik
salınımlarının ısı transferi üzerindeki etkisi deneysel ve sayısal olarak
incelenmiştir. Deney sistemi, içinde deneysel modelin hareket ettiği şeffaf bir muhafazayı, modelin salınım hareketini üreten volan-motor sistemini,
güç kaynağını, veri toplama sistemini ve bilgisayarı içerir. Deneysel model,
üzerine termoelemanlar yerleştirilmiş iki bakır levha ve levhalar arasına
yerleştirilmiş Kapton ısıtıcılardan oluşur. Çalışmada, levhanın yüzeyine
uygulanan ısı akısı (q″), Womersley sayısı (Wo) ve boyutsuz salınım genliği
(Ao) değiştirilmiş ve bu parametrelerin ısı transferi üzerindeki etkisi analiz
edilmiştir. Ayrıca, çalışma deneysel verilere dayalı kontrol hacim tabanlı bir
HAD çözücüsü kullanılarak sayısal olarak çözülmüştür. Sayısal sonuçlar,
deneysel sonuçlarla karşılaştırılmıştır. Salınım genliği ve frekansının ısı
transferine etkisini göstermek için levha yüzeyinde anlık hız ve sıcaklık görüntüleri
elde edilmiştir. Sayısal ve deneysel sonuçlar, ısı transferinin salınım parametrelerinden
önemli derecede etkilendiğini ve salınım genliği ve frekansının artması ile arttığını
göstermiştir.
The Experienced Level of Stress and Anxiety in Patients of a Multidisciplinary Medical Center
Authors: Kholmogorova A. B. , Subotich M. I. , Rakhmanina A. A. , Borisonik E. V. , Roy A. P. , Lysenko Y. S. , Petrikov S. S. 
Published: January 2020

At the present stage of development of medicine, the delivery of psychological assistance is an important component of a systemic biopsychosocial approach. Patients and persons accompanying them undoubtedly experience anxiety during hospitalization due to the fact that the very appeal to the hospital and the preceding events are stressful. In turn, many previous studies show that stress can have both mobilizing (eustress) and negative effects (distress) on the emotional state and adaptive processes of a person.The objective of this study is to study the level of stress and anxiety in patients (n=83) at the time of hospitalization and hospital stay. The study was conducted using three self-reporting scales (the Distress Thermometer, A. Beck Anxiety Inventory, and Hospital Anxiety and Depression Scale (HADS). The results of the study showed that most patients (58%) experienced an increased level of distress. Distress was most often accompanied by the following emotional reactions: anxiety (51%), fear (25%), sadness (21%), and loneliness (21%). The study of emotional state showed that 22% of respondents had a high level of anxiety (according to HADS), and 5% had clinically significant symptoms of anxiety (according to Beck Inventory). Symptoms of depression are less common. In the group of patients with a significant level of distress, a greater number of patients noted high rates of hospital anxiety and depression. In addition, the authors investigated the emotional state of patients after psychological assistance received in the hospital. The results show that the level of distress was almost twice reduced.A pilot study of a group of patients being treated in the clinical departments of an emergency hospital allows us to draw a preliminary conclusion about the positive effect of the course of psychocorrective classes on the patient’s emotional state and the level of distress experienced, i.e. helps the patient resolve certain internal conflicts associated with a sudden change in life, calm down and positively set oneself up for treatment.Authors declare lack of the conflicts of interests.