bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epithelial invagination by a vertical telescoping cell movement in mammalian salivary glands and teeth
Authors: Li Jingjing , Economou Andrew D. , Vacca Barbara , Green Jeremy B. A. 
Published: May 2020

AbstractEpithelial bending is a fundamental process that shapes organs during development. Previously known mechanisms involve cells locally changing shape from columnar to wedge-shaped. Here we report a different mechanism that occurs without cell wedging. In mammalian salivary glands and teeth, we show that initial invagination occurs through coordinated vertical cell movement: cells towards the periphery of the placode move vertically upwards while their more central neighbours move downwards. Movement is achieved by active cell-on-cell migration: outer cells migrate with apical, centripetally polarised leading edge protrusions but remain attached to the basal lamina, depressing more central neighbours to “telescope” the epithelium downwards into underlying mesenchyme. Inhibiting protrusion formation by Arp2/3 protein blocks invagination. FGF and Hedgehog morphogen signals are required, with FGF providing a directional cue. These findings show that epithelial bending can be achieved by a morphogenetic mechanism of coordinated cell rearrangement quite distinct from previously recognised invagination processes.
Acute Stress Reduces the Social Amplification of Risk Perception
Authors: Popovic Nathalie F. , Bentele Ulrike U. , Pruessner Jens C. , Moussaïd Mehdi , Gaissmaier Wolfgang 
Published: May 2020

AbstractRisk perceptions typically underlie a complex social dynamic: Risk-related information is transmitted between individuals, this information influences risk perceptions, and risk perceptions influence which information is transmitted. This can lead to a social amplification of risk. We test how stress, a widespread affective state, influences the social dynamics of risk perception. Participants (N = 146) read articles about the controversial antibacterial agent Triclosan and were then asked to inform another person about Triclosan. Before and after reading the articles, participants reported their concern about Triclosan. Stress exposure before the task led to a smaller increase in concern in response to the articles. The stronger the increase in cortisol, the smaller the increase in concern. Furthermore, participants in the stress group transmitted less negative information about Triclosan to others. In contrast, participants’ subjective feelings of stress were associated with higher concern and more alarming risk communication. We conclude that feeling stressed can amplify risk perception, whereas the endocrine stress reaction can attenuate risk perception when information about risk is exchanged in a social context.
Equine penile squamous cell carcinoma: expression of biomarker proteins and EcPV2
Authors: Arthurs Callum , Suarez-Bonnet Alejandro , Willis Claire , Xie Boyu , Machulla Natalie , Mair Tim S. , Cao Kevin , Millar Michael , Thrasivoulou Christopher , Priestnall Simon L. , Ahmed Aamir 
Published: May 2020

AbstractEquine penile squamous cell carcinoma (EpSCC) is a relatively common cutaneous neoplasm with a poor prognosis. In this study, we aimed to determine the protein expression and colocalisation of FRA1, c-Myc, Cyclin D1, and MMP7 in normal (NT), tumour (T), hyperplastic epidermis and/or squamous papilloma (Hyp/Pap), poorly-differentiated (PDSCC), or well-differentiated (WDSCC) EpSCC using a tissue array approach. Further objectives were to correlate protein expression to (i) levels of inflammation, using a convolutional neural network (ii) equine papillomavirus 2 (EcPV2) infection, detected using PCR amplification. We found an increase in expression of FRA1 in EpSCC compared to NT samples. c-Myc expression was higher in Hyp/Pap and WDSCC but not PDSCC whereas MMP7 was reduced in WDSCC compared with NT. There was a significant increase in the global intersection coefficient (GIC) of FRA1 with MMP7, c-Myc, and Cyclin D1 in EpSCC. Conversely, GIC for MMP7 with c-Myc was reduced in EpSCC tissue. Inflammation was positively associated with EcPV2 infection in both NT and EpSCC but not Hyp/Pap. Changes in protein expression could be correlated with EcPV2 for Cyclin D1 and c-Myc. Our results evaluate novel biomarkers of EpSCC and a putative correlation between the expression of biomarkers, EcPV2 infection and inflammation.
Modelling hepatitis D virus RNA and HBsAg dynamics during nucleic acid polymer monotherapy suggest rapid turnover of HBsAg
Authors: Shekhtman Louis , Cotler Scott J. , Hershkovich Leeor , Uprichard Susan L. , Bazinet Michel , Pantea Victor , Cebotarescu Valentin , Cojuhari Lilia , Jimbei Pavlina , Krawczyk Adalbert , Dittmer Ulf , Vaillant Andrew , Dahari Harel 
Published: May 2020

AbstractHepatitis D virus (HDV) requires hepatitis B surface antigen (HBsAg) for its assembly and release. Current HBV treatments are only marginally effective against HDV because they fail to inhibit HBsAg production/secretion. However, monotherapy with the nucleic acid polymer REP 2139-Ca is accompanied by rapid declines in both HBsAg and HDV RNA. We used mathematical modeling to estimate HDV-HBsAg-host parameters and to elucidate the mode of action and efficacy of REP 2139-Ca against HDV in 12 treatment-naive HBV/HDV co-infected patients. The model accurately reproduced the observed decline of HBsAg and HDV, which was simultaneous. Median serum HBsAg half-life (t1/2) was estimated as 1.3 [0.9–1.8] days corresponding to a pretreatment production and clearance of ~108 [107.7–108.3] IU/day. The HDV-infected cell loss was estimated to be 0.052 [0.035–0.074] days−1 corresponding to an infected cell t1/2 = 13.3 days. The efficacy of blocking HBsAg and HDV production were 98.2 [94.5–99.9]% and 99.7 [96.0–99.8]%, respectively. In conclusion, both HBsAg production and HDV replication are effectively inhibited by REP 2139-Ca. Modeling HBsAg kinetics during REP 2139-Ca monotherapy indicates a short HBsAg half-life (1.3 days) suggesting a rapid turnover of HBsAg in HBV/HDV co-infection.
Human multi-organ chip co-culture of bronchial lung culture and liver spheroids for substance exposure studies
Authors: Schimek Katharina , Frentzel Stefan , Luettich Karsta , Bovard David , Rütschle Isabel , Boden Laura , Rambo Felix , Erfurth Hendrik , Dehne Eva-Maria , Winter Annika , Marx Uwe , Hoeng Julia 
Published: May 2020

AbstractExtrapolation of cell culture-based test results to in vivo effects is limited, as cell cultures fail to emulate organ complexity and multi-tissue crosstalk. Biology-inspired microphysiological systems provide preclinical insights into absorption, distribution, metabolism, excretion, and toxicity of substances in vitro by using human three-dimensional organotypic cultures. We co-cultured a human lung equivalent from the commercially available bronchial MucilAir culture and human liver spheroids from HepaRG cells to assess the potential toxicity of inhaled substances under conditions that permit organ crosstalk. We designed a new HUMIMIC Chip with optimized medium supply and oxygenation of the organ cultures and cultivated them on-chip for 14 days in separate culture compartments of a closed circulatory perfusion system, demonstrating the viability and homeostasis of the tissue cultures. A single-dose treatment of the hepatotoxic and carcinogenic aflatoxin B1 impaired functionality in bronchial MucilAir tissues in monoculture but showed a protective effect when the tissues were co-cultured with liver spheroids, indicating that crosstalk can be achieved in this new human lung–liver co-culture. The setup described here may be used to determine the effects of exposure to inhaled substances on a systemic level.
Extracellular Signal-Regulated Kinases Mediate an Autoregulation of GABAB-Receptor-Activated Whole-Cell Current in Locus Coeruleus Neurons
Authors: Wu Rui-Ni , Kuo Chao-Cheng , Min Ming-Yuan , Chen Ruei-Feng , Yang Hsiu-Wen 
Published: May 2020

AbstractThe norepinephrine-releasing neurons in the locus coeruleus (LC) are well known to regulate wakefulness/arousal. They display active firing during wakefulness and a decreased discharge rate during sleep. We have previously reported that LC neurons express large numbers of GABABreceptors (GABABRs) located at peri-/extrasynaptic sites and are subject to tonic inhibition due to the continuous activation of GABABRs by ambient GABA, which is significantly higher during sleep than during wakefulness. In this study, we further showed using western blot analysis that the activation of GABABRs with baclofen could increase the level of phosphorylated extracellular signal-regulated kinase 1 (ERK1) in LC tissue. Recordings from LC neurons in brain slices showed that the inhibition of ERK1/2with U0126 and FR180204 accelerated the decay of whole-cell membrane current induced by prolonged baclofen application. In addition, the inhibition of ERK1/2also increased spontaneous firing and reduced tonic inhibition of LC neurons after prolonged exposure to baclofen. These results suggest a new role of GABABRs in mediating ERK1-dependent autoregulation of the stability of GABABR-activated whole-cell current, in addition to its well-known effect on gated potassium channels, to cause a tonic current in LC neurons.
Photocatalytic dye degradation and antimicrobial activities of Pure and Ag-doped ZnO using Cannabis sativa leaf extract
Authors: Chauhan Ankush , Verma Ritesh , Kumari Swati , Sharma Anand , Shandilya Pooja , Li Xiangkai , Batoo Khalid Mujasam , Imran Ahamad , Kulshrestha Saurabh , Kumar Rajesh 
Published: May 2020

AbstractA facile green route has been employed for the synthesis of ZnO and Ag-doped ZnO using Cannabis sativa as a reducing and stabilizing agent. The as-synthesized nanoparticles were characterized and tested for photocatalytic dye degradation and antimicrobial activity. The results suggested that nanoparticles have shown antimicrobial activity against different human pathogenic bacteria (Escherichia coli, Klebsiella pneumonia, MRSA, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus) and fungal strains (Fusarium spp. and Rosellinia necatrix). Ag-doped nanoparticles comparatively have shown better removal Congo red and methyl orange under visible light. Therefore, green synthesized nanoparticles could have beneficial applications in environmental science and biological field.
Universal principles justify the existence of concept cells
Authors: Calvo Tapia Carlos , Tyukin Ivan , Makarov Valeri A. 
Published: May 2020

AbstractThe widespread consensus argues that the emergence of abstract concepts in the human brain, such as a “table”, requires complex, perfectly orchestrated interaction of myriads of neurons. However, this is not what converging experimental evidence suggests. Single neurons, the so-called concept cells (CCs), may be responsible for complex tasks performed by humans. This finding, with deep implications for neuroscience and theory of neural networks, has no solid theoretical grounds so far. Our recent advances in stochastic separability of highdimensional data have provided the basis to validate the existence of CCs. Here, starting from a few first principles, we layout biophysical foundations showing that CCs are not only possible but highly likely in brain structures such as the hippocampus. Three fundamental conditions, fulfilled by the human brain, ensure high cognitive functionality of single cells: a hierarchical feedforward organization of large laminar neuronal strata, a suprathreshold number of synaptic entries to principal neurons in the strata, and a magnitude of synaptic plasticity adequate for each neuronal stratum. We illustrate the approach on a simple example of acquiring “musical memory” and show how the concept of musical notes can emerge.
Endosulfan exposure alters transcription of genes involved in the detoxification and stress responses in Physella acuta
Authors: Alonso-Trujillo María , Muñiz-González Ana-Belén , Martínez-Guitarte José-Luis 
Published: May 2020

AbstractEndosulfan is a persistent pesticide that has been in use for more than five decades. During this time, it has contaminated soil, air, and water reservoirs worldwide. It is extremely toxic and harmful to beneficial non-target invertebrates, aquatic life, and even humans upon consumption, which is one of the many dangers of this pesticide since it biomagnifies in the food chain. The effects of three endosulfan concentrations (1, 10, and 100 µg/L) on the freshwater snail Physella acuta, an invasive cosmopolitan species, were examined over a week-long exposure period. Alterations in the expression of ten genes related to stress and xenobiotic detoxification were measured against the endogenous controls rpL10 and GAPDH by Real-Time polymerase chain reaction. Four genes are described here for the first time in this species, namely Hsp60, Grp78, GSTk1, and GSTm1. The rest of genes were Hsp90, sHsp16.6, cyp2u1, cyp3a7, cyp4f22, and MRP1. cyp2u1, sHsp16.6, and Grp78 expression were all altered by endosulfan. These results suggest a low pesticide concentration activates the acute response in P. acuta by affecting detoxification and stress responses and alter endoplasmic reticulum function and lipid metabolism. Furthermore, the newly identified genes extend the number of processes and cellular locations that can be analyzed in this organism.
Elevated primary productivity triggered by mixing in the quasi-cul-de-sac Taiwan Strait during the NE monsoon
Authors: Huang Ting-Hsuan , Chen Chen-Tung Arthur , Bai Yan , He Xianqiang 
Published: May 2020

AbstractThe Taiwan Strait (TS) connects two of the largest marginal seas in the world, namely the East China Sea (ECS) and the South China Sea (SCS). When the NE monsoon prevails, the fresh, nutrient-rich but P-limited China Coastal Current (CCC) flows southward. Yet, part of the CCC turns eastward after entering the TS and then turns back toward the ECS. In the southern TS, part of the salty, N-limited, northward TS current (TSC) in the eastern part of the strait turns westward and eventually returns to the SCS. That is, the TS acts like a quasi-cul-de-sac during the NE monsoon season. Based on 822 samples from 28 cruises, the highest Chl. a concentration occurs at a salinity around 32 even though the nutrient concentration is not the highest. Mixing the cold-fresh-eutrophic CCC water and the warm-salty-oligotrophic TSC water results in a more suitable condition for biological uptake in both the southern ECS and the northern SCS.
Seasonal fluctuation of beak and feather disease virus (BFDV) infection in wild Crimson Rosellas (Platycercus elegans)
Authors: Martens Johanne M. , Stokes Helena S. , Berg Mathew L. , Walder Ken , Bennett Andrew T. D. 
Published: May 2020

AbstractUnderstanding patterns of pathogen emergence can help identify mechanisms involved in transmission dynamics. Beak and feather disease virus (BFDV) poses a major threat world-wide to wild and captive parrots. Yet data from wild birds on seasonal fluctuations in prevalence and infection intensity, and thereby the potential high-risk times for virus transmission, have been lacking. We screened wild Crimson Rosellas (Platycercus elegans) for BFDV in blood and cloacal swabs. Prevalence in blood samples and cloacal swabs, as well as viral load varied with Julian date and in blood, were highest after the breeding season. Breeding birds had lower viral load and lower BFDV prevalence in blood than non-breeding birds (10.1% prevalence in breeding vs. 43.2% in non-breeding birds). BFDV prevalence was much higher in younger (<3 years) than older (≥3 years) birds for both blood samples (42.9% vs. 4.5%) and cloacal swabs (56.4% vs. 12.3%). BFDV status in blood and cloacal samples was not correlated within individuals. We show that, at least inP. elegans, BFDV infection seems to occur year-round, with seasonal changes in prevalence and load found in our samples. Our analyses suggest that the seasonal changes were associated primarily with the breeding season. We also discuss age and sex as important predictors of BFDV infection.
Association analysis in a Latin American population revealed ethnic differences in rheumatoid arthritis-associated SNPs in Caucasian and Asian populations
Authors: Castro-Santos P. , Verdugo R. A. , Alonso-Arias R. , Gutiérrez M. A. , Suazo J. , Aguillón J. C. , Olloquequi J. , Pinochet C. , Lucia A. , Quiñones L. A. , Díaz-Peña R. 
Published: May 2020

AbstractLarge genome-wide association studies (GWAS) have increased our knowledge of the genetic risk factors of rheumatoid arthritis (RA). However, little is known about genetic susceptibility in populations with a large admixture of Amerindian ancestry. The aim of the present study was to test the generalizability of previously reported RA loci in a Latin American (LA) population with admixed ancestry. We selected 128 single nucleotide polymorphisms (SNPs) in linkage equilibrium, with high association to RA in multiple populations of non-Amerindian origin. Genotyping of 118 SNPs was performed in 313 RA patients/487 healthy control subjects by mid-density arrays of polymerase chain reaction (PCR). Some of the identified associations were validated in an additional cohort (250 cases/290 controls). One marker, the SNP rs2451258, located upstream of T Cell Activation RhoGTPase Activating Protein (TAGAP) gene, showed significant association with RA (p = 5 × 10−3), whereas 18 markers exhibited suggestive associations (p < 0.05). Haplotype testing showed association of some groups of adjacent SNPs around the signal transducer and activator of transcription 4 (STAT4) gene (p = 9.82 × 10−3 to 2.04 × 10−3) with RA. Our major finding was little replication of previously reported genetic associations with RA. These results suggest that performing GWAS and admixture mapping in LA populations has the potential to reveal novel loci associated with RA. This in turn might help to gain insight into the ‘pathogenomics’ of this disease and to explore trans-population differences for RA in general.
Structural investigation of borosilicate glasses containing lanthanide ions
Authors: Fabian M. , Gergely F. , Osan J. , Cendak T. , Kesari S. , Rao R. 
Published: May 2020

AbstractHigh level radioactive actinides are produced as a side product in reprocessing spent nuclear fuel, for which safe long-term-inert immobilizer matrices are needed. Borosilicate glasses are of great potential amongst the candidates of suitable inert materials for radioactive waste immobilization. Understanding the effects of actinide addition to a borosilicate glass matrix is of great importance in view of waste immobilization. Here we present structural studies of a simplified glass-matrix, − 55SiO2·10B2O3·25Na2O·5BaO·5ZrO2 - upon adding lanthanide (Ln-)oxides: CeO2, Nd2O3, Eu2O3, in two different concentrations 10% and 30w% each, to investigate the effects of lanthanides (Ln) taken as chemical surrogates for actinides. Neutron diffraction combined with of Reverse Monte Carlo simulations show that all investigated glass structures comprise tetrahedral SiO4, trigonal BO3 and tetrahedral BO4 units, forming mixed [4]Si-O-[3]B and [4]Si-O-[4]B linkages. 11B Magic Angle Spinning Nuclear Magnetic Resonance is indicative of simultaneous presence of trigonal BO3 and tetrahedral BO4 units, with spectral fractions strongly dependent on the Ln addition. Ln-addition promote the BO3 + O-→[BO4]– isomerization resulting in lower fraction of boron in BO3, as compared to BO4 units. Raman spectra, in full agreement with neutron diffraction, confirm that the basic network structure consists of BO3/trigonal and SiO4/BO4 tetrahedral units. Second neighbour atomic pair correlations reveal Ce, Nd, Eu to be accommodated in both Si and B sites, supporting that the borosilicate-matrix well incorporates Ln-ions and is likely to similarly incorporate actinides, opening a way to radioactive nuclear waste immobilization of this group of elements in a borosilicate glass matrix.
Clinical efficacy of Stereotactic Body Radiation Therapy (SBRT) for adrenal gland metastases: A multi-center retrospective study from China
Authors: Zhao Xianzhi , Zhu Xiaofei , Zhuang Hongqing , Guo Xueling , Song Yongchun , Ju Xiaoping , Wang Ping , Yuan Zhiyong , Zhang Huojun 
Published: May 2020

AbstractTo evaluate the efficacy and safety of CyberKnife Stereotactic Body Radiation Therapy (SBRT) in the treatment of adrenal gland metastases (AGM), we designed a large-scale multicenter retrospective study to report the safety and efficacy of SBRT for inoperable AGM. In this study, 75 (61 males, 14 females) patients with 84 AGM and Karnofsky performance score ≥70 were treated by SBRT from October 2006 to January 2017. Of these, the purpose of treatment were controlling all known metastatic sites for 21 patients while 54 for palliation of bulky adrenal metastases. The efficacy and safety of SBRT were evaluated during follow-up. Potential factors predictive of local control (LC) and overall survival (OS) were identified by univariate and multivariate analysis. Median follow-up time was 12.7 months (range 1.8–96.4). The complete response (CR), partial response (PR), stable disease (SD) and progressive disease (PD) rates were 23.8%, 23.8%, 31.0% and 21.4%, respectively. The 0.5, 1, and 2-year LC rates were 93.6%, 83.8%, and 62.1%, respectively; OS rates on the same follow-up intervals were 93.7%, 62.5%, and 49.6%, respectively, and the corresponding PFS rates were 48.5%, 33.9%, and 16.0%, respectively. The treatment was well tolerated with 2 patients reporting grade-3 diarrhea and fatigue, respectively. Multivariate analysis showed that simultaneous treatment of SBRT for other metastatic lesions, the number of AGM, initiation of systemic therapy, and the maximum diameter of AGM were predictive of LC rates. Moreover, patients with AGM < 5 cm had a superior OS compared with those with AGM ≥ 5 cm (28.0 months vs. 17.6 months, P = 0.032). SBRT is an effective therapeutic option for treatment of AGM with high LC rates with minimal toxicity.
Stokes flow around an obstacle in viscous two-dimensional electron liquid
Authors: Gusev G. M. , Jaroshevich A. S. , Levin A. D. , Kvon Z. D. , Bakarov A. K. 
Published: May 2020

AbstractThe electronic analog of the Poiseuille flow is the transport in a narrow channel with disordered edges that scatter electrons in a diffuse way. In the hydrodynamic regime, the resistivity decreases with temperature, referred to as the Gurzhi effect, distinct from conventional Ohmic behaviour. We studied experimentally an electronic analog of the Stokes flow around a disc immersed in a two-dimensional viscous liquid. The circle obstacle results in an additive contribution to resistivity. If specular boundary conditions apply, it is no longer possible to detect Poiseuille type flow and the Gurzhi effect. However, in flow through a channel with a circular obstacle, the resistivity decreases with temperature. By tuning the temperature, we observed the transport signatures of the ballistic and hydrodynamic regimes on the length scale of disc size. Our experimental results confirm theoretical predictions.