bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trophic states regulate assembly processes and network structures of small chromophytic phytoplankton communities in subtropical coastal ecosystem
Authors: Chen Xing , Zhao Huaxian , Jiang Gonglingxia , Tang Jinli , Xu Qiangsheng , Chen Si , Zou Shuqi , Dong Ke , Hou Weiguo , Pan Lianghao , Wang Pengbin , Li Nan 
Published: October 2020

Abstract
Small chromophytic phytoplankton (SCP) are anticipated to be more important for a significant proportion of primary production in estuarine-coastal ecosystems. However, responses of SCP community to coastal eutrophication are still unclear. In this study, we investigated diversity, co-occurrence and assembly features of SCP communities, as well as their relationship with environmental factors in the subtropical Beibu Gulf. The results exhibited that the alpha diversity (ANOVA, p < 0.001) and beta diversity (ANOSIM, p < 0.001) of SCP communities were significantly different among eutrophic states. Co-occurrence network analysis revealed a complex interaction between SCP community and environmental factors. Most ASVs in modules of the network were specific to trophic states. Further, phylogenetic based β-nearest taxon distance analyses revealed that stochastic processes mainly provided 69.26% contribution to SCP community assembly, whereas deterministic processes dominated community assembly in a heavy eutrophic state. Importantly, increased environmental disturbances, such as nitrogen and phosphorus nutrients, could alter SCP community structure and disrupt ecological processes. Overall, our findings elucidate the mechanism of diversity and assembly in marine SCP community and promote the understanding of SCP ecology related to subtropical coastal eutrophication.
To Find Your Location: Secure and Practical Point Location Scheme
Authors: Guo Jingjing , Sun Jiacong 
Published: October 2020

Abstract
With the ubiquitous mobile devices and the advanced wireless communication, location-based service (LBS) helps people to enjoy a convenient lifestyle and has attracted numerous research interests. As a basic query process in LBS system, point location requires to find a region containing the query point. Since location belongs to sensitive information and also leads other private information leaked, it is urgent to design a secure and efficient point location scheme. In this paper, we propose a point location scheme named SecPL to protect sensitive information while supporting high efficient location query. Specifically, we introduce a LineTest scheme from asymmetric scalar-product-preserving encryption (ASPE) to facilitate the checking of whether a point lies above or below a line. Furthermore, the SecPL scheme is designed by leveraging LineTest and order-preserving encryption (OPE) scheme. Through detailed security analysis, we demonstrate that SecPL scheme achieves data privacy and query privacy at the same time. Finally, the performance evaluation demonstrates the high efficiency of the proposed SecPL scheme.
Assessing land use and land cover changes and Effects of Large-scale Agricultural Farm Expansion in Southwester Ethiopia by using remote sensing/GIS tools.
Authors: Shoddo Getaneh Haile 
Published: October 2020

Abstract
Development initiatives like the recent increase in large-scale investment agriculture have made a significant impact on the forest. In the name of development, the land is often given to investors often in long-term leases and at bargain prices. Research on deforestation has been mostly restricted to poverty and population growth as the driving forces for tropical deforestation; however, explanations emphasizing market factors such as increases in large-scale investment agriculture as a cause of deforestation have only been carried out in a small number of areas. The aim of this study is to explore the effects of agricultural land expansion in changing land use and land use cover changes using remote sensing/GIS tools in Sheka zone southwester Ethiopia from 1995 to 2015. The results showed that expansion of investment agriculture has a clear impact on both the local people and the forest ecosystem. The conversion of forestland to investment agriculture has caused varied and extensive environmental degradation to the Sheka forest. The Land Use and Land Cover changes in the Sheka zone are discussed based on underlying socioeconomic factors.
Genome-wide phylogenetic analysis, expression pattern, and transcriptional regulatory network of the pig C/EBP gene family
Authors: Zhang Chaoxin , Wang Tao , Liu Shengwei , Zhang Bing , Li Xue , Tang Jian , Wang Peng , Guo Yuanyuan , Wang Zhipeng 
Published: October 2020

Abstract
Background: The vertebrate C/EBP transcription factors regulate many important biological processes, such as cell proliferation, differentiation, signal transduction, inflammation, and energy metabolism. The first C/EBP protein was identified in rat liver nuclei. Development of sequencing technology resulted in identification of the C/EBP genes in various species. In this study, a bioinformatics approach was used to determine the distribution of the members of the C/EBP family in vertebrates. A phylogenetic tree was constructed to analyze the C/EBP genes in vertebrates. Based on RNA-seq data, the expression patterns of pig C/EBP members in various tissues were analyzed. In addition, a gene transcription regulatory network was constructed with pig C/EBP members as the core.Results: We identified a total of 92 C/EBP genes in 17 vertebrate genomes. Phylogenetic analysis showed that all C/EBP TFs were classified into two groups; group I contained C/EBPβ TFs, and group II contained the remaining C/EBP TFs. The C/EBPα, C/EBPβ, C/EBPδ, C/EBPγ, and C/EBPζ genes were expressed ubiquitously with inconsistent expression patterns in various tissues. Moreover, a pig C/EBP regulatory network was constructed, including C/EBP genes, TFs, and miRNAs. A total of 39 FFL motifs were detected in the pig C/EBP regulatory network. Based on the RNA-seq data, gene expression patterns related to this FFL sub-network were analyzed in 27 adult Duroc tissues. Certain FFL motifs may be tissue specific. Functional enrichment analysis indicated that C/EBP and its target genes are involved in many important biological pathways. Conclusions: These results provide valuable information that clarifies the evolutionary relationships of the C/EBP family and contributes to the understanding of the biological function of C/EBP genes.
Feature-based Energy Consumption Quantitation Strategy for Additive Manufacturing of Complex Parts
Authors: Gao Mengdi , Liu Conghu , Wang Qingyang , Li Lei , Li Xinyu , Ma Zhilin , Liu Zhifeng 
Published: October 2020

Abstract
Additive manufacturing (AM) has significant advantages over conventional manufacturing technologies, including freedom of design, mass customization, and the ability to produce complex structures. Because reducing manufacturing energy is an essential challenge for industrial sustainability and national economics, the trend toward AM motivates us to explore the energy consumption of AM to address energy efficiency. Existing energy consumption quantitation methods for AM parts require complex models, and the energy consumption characteristics of AM equipment muse be considered in the quantitative analysis process. Therefore, a feature-based energy consumption quantitation method for complex AM parts is proposed that uses a simple model and can be applied to different AM technologies. A feature segmentation method is first proposed to divide complex AM parts into typical AM features (AMFs). Then, the energy consumption model is developed for each AMF to quantify the energy consumption during fabrication of the entire part. Finally, the energy consumption characteristics of a typical mechanical part manufactured by three different kinds of AM processes—fuse deposition modeling (FDM), stereolithography (SLA), and selective laser melting (SLM)—are investigated using the proposed feature-based energy consumption quantitation method and measured in an experimental case study. The results show that the proposed method can effectively and quickly predict the energy consumption of AM part manufacturing. Moreover, the efficiency of different types of AM processes is compared and discussed to address applicable efficiency improvement methods. This method can predict the energy consumption of complex AM parts, and can be integrated into the AM three-dimensional software model, providing a reference for structural optimization of AM parts.
Construction and Validation of a Metabolic Reprogramming Related Prognostic Model for Hepatocellular Carcinoma
Authors: Liu Xiaohong - , Xu Qian - , Li Zi-Jing - , Xiong Bin - 
Published: October 2020

Abstract
BackgroundMetabolic reprogramming is an important hallmark in the development of malignancies. Numerous metabolic genes have been demonstrated to participate in the progression of hepatocellular carcinoma (HCC). However, the prognostic significance of the metabolic genes in HCC remains elusive. MethodsWe downloaded the gene expression profiles and clinical information from the GEO, TCGA and ICGC databases. The differently expressed metabolic genes were identified by using Limma R package. Univariate Cox regression analysis and LASSO (Least absolute shrinkage and selection operator) Cox regression analysis were utilized to uncover the prognostic significance of metabolic genes. A metabolism-related prognostic model was constructed in TCGA cohort and validated in ICGC cohort. Furthermore, we constructed a nomogram to improve the accuracy of the prognostic model by using the multivariate Cox regression analysis.ResultsThe high-risk score predicted poor prognosis for HCC patients in the TCGA cohort, as confirmed in the ICGC cohort (P < 0.001). And in the multivariate Cox regression analysis, we observed that risk score could act as an independent prognostic factor for the TCGA cohort (HR (hazard ratio) 3.635, 95% CI (confidence interval)2.382-5.549) and the ICGC cohort (HR1.905, 95%CI 1.328-2.731). In addition, we constructed a nomogram for clinical use, which suggested a better prognostic model than risk score.ConclusionsOur study identified several metabolic genes with important prognostic value for HCC. These metabolic genes can influence the progression of HCC by regulating tumor biology and can also provide metabolic targets for the precise treatment of HCC.
SIMS (Imaging Secondary Ion Mass Spectrometry) Analysis of a Cross-section Sample From Leonardo Da Vinci’s Adoration of the Magi
Authors: Tian Hua , Seracini Maurizio , Schimmel Katherine , Benkovic Stephen J. , Winograd Nicholas 
Published: October 2020

Abstract
Paintings often consist of highly complex layered structures that contain a mixture of organic and inorganic materials at each layer depending upon the artist’s technique. A comprehensive analysis of the chemical composition could provide critical information on the sequence of events that led to an artwork’s current state. In this investigation, we have employed a novel imaging technique known as Secondary Ion Mass Spectrometry (SIMS) to chemically map the cross-section of a paint film sampled from Leonardo da Vinci’s Adoration of the Magi at a submicron resolution using a C60+ ion source. A wide range of materials were found to be spatially localized at the different layers such as: protein, carbon, silicates, fatty acids, salts and lead associated compounds. An interaction of chloride salts with the lead white was observed in the priming layer. The chloride salts disrupted most of the priming layer suggesting that a cleaning process took place that removed most of the priming layer before the monochrome brown repaints were added on top at a later time.
Low or Moderate Intensity Aerobic Training: Its Role in Clinical and Psychological Aspects of Community Dwelling COVID-19 Asymptomatic Older Adults With Sarcopenia Symptoms
Authors: Nambi Gopal , Abdelbasset Walid Kamal , Verma Anju , Vellaiyan Arul 
Published: October 2020

Abstract
Background: Sarcopenia is the major health concern and common consequence of COVID-19 in the aging population. Moreover, this clinical condition has not been considered in usual physical rehabilitation practice and nor its exercise protocol is well defined, which requires a meaningful study in this field.Objective: To find and compare the low and moderate intensity aerobic training protocols on clinical and psychological effects in community dwelling COVID-19 asymptomatic older adults with Sarcopenia symptoms. Methods: By using computer random table method the eligible participants were randomized into two groups. First group received low-intensity aerobic training (LAT; n = 38) and the second group received moderate-intensity aerobic training (MAT; n = 38) for 8 weeks. Clinical (muscle strength, muscle mass and physical performance) and psychological (kinesiophobia and quality of life) measures were measured at baseline, at 4th week, 8th week and at 6 month follow up. Results: Baseline demographic and clinical attributes show homogenous presentation among the study groups (p>0.05). After eight weeks of different aerobic trainings, and at the end of 6 months follow up, the hand grip strength, -3.0 (CI 95% -4.16 to -1.83), chair stand test -2.7 (-3.29 to -2.10), physical performance -0.08 (-0.10 to -0.05), kinesiophobia, 4.2 (3.25 to 5.14), and quality of life -5.7 (-8.4 to -2.9) shows more improvement (p<0.001) in LAT group than MAT group but in muscle mass both groups did not show any significant difference (p>0.05).Conclusion: This study reports that low intensity aerobic training exercises improve the clinical (muscle strength and physical performance) and psychological (kinesiophobia and quality of life) aspects than moderate intensity aerobic training in COVID-19 asymptomatic older adults with Sarcopenia symptoms. At the same time both types of aerobic training exercises have negligible or little role in increasing the muscle quantity - cross sectional area.
Angelica Sinensis Polysaccharides Ameliorate 5-Flourouracil-Induced Bone Marrow Stromal Cell Proliferation Inhibition Via Regulating Wnt/ β-Catenin Signaling
Authors: Xiao Hanxianzhi , Qi Rongjia , Wang Ziling , Xiao Minghe , Xiang Yue , Wang YaPing , Wang Lu 
Published: October 2020

Abstract
Background: Chemotherapy-induced bone marrow hematopoietic microenvironment oxidative damage is closely related to myelosuppression. Angelica Sinensis Polysaccharides (ASP) are major effective ingredients of traditional Chinese medicine Angelica with multi-target anti-oxidative stress features. In this study, we investigated the potential benefits and mechanism of action of ASP on chemotherapy-induced bone marrow stromal cell (BMSC) damage.Methods: The human bone marrow stromal cell line HS-5 cells were divided into control group, 5-FU group, 5-FU + ASP group, and 5-FU+ LiCl group to investigate the mechanism of ASP to alleviate 5-FU-induced BMSC proliferation inhibition.Results: The results showed that 5-FU inhibits the growth of HS-5 cells in a time and dose-dependent manner; however, ASP partially counteracted 5-FU-induced decrease in cell viability, whereas Wnt signaling inhibitor Dkk1 antagonized the effect of ASP on HS-5 cells. ASP reversed the decrease in cytoplasmic total β-catenin, p-GSK-3β, and CyclinD1 following 5-FU treatment, and modulated nuclear expression of β-catenin, Lef-1, and C-myc proteins. Furthermore, ASP also enhanced the antioxidant capacity of cells and reduced 5-FU caused oxidative stress, attenuated FoxO1 expression, thus weakened its downstream apoptosis-related proteins and G0/G1 checkpoint-associated p27Kip1expression to alleviate 5-FU-induced apoptosis promote cell cycle progression. Conclusion: The protective role of ASP in BMSCs proliferation for the chemotherapy may be related to its activating Wnt/β-catenin signaling and keeping homeostasis between β-catenin and FoxO1 under oxidative stress. The study provides a potential therapeutic strategy for alleviating chemotherapeutic damage on BMSCs.
Development of a Mobile-App&nbsp;Based Free Field Click Test Against Conventional Free Field Voice Test: A Randomized Controlled Trial
Authors: Rajendran Thilaga , Chye Julius Goh Liang , Shanmuganathan Jothi 
Published: October 2020

Abstract
Objective:Hearing loss is a prevalent disease with free field voice test (FFVT) being a common hearing test performed.FFVT has its own disadvantage as screening tool as there is no standardization of speech among examiners.We aim to invent mobile-app based hearing screening using Flutter-Dart programming language that mimics findings of FFVT.The app is termed as Free field click test (FFCT).Our aim is to compare sensitivity&specificity of FFCT and FFVT with Pure Tone audiogram (PTA).Methods:This is an open-labelled randomized controlled trial. This study was conducted in a sound proof room in our ORL clinic from December-March 2020. 50 patients from 18-80 years old with complaint of hearing loss were recruited.These patients were randomly assigned into two groups; FFVT&FFCT which were compared with PTA.Categorical data were analyzed with Chi-square test,non-categorical data were calculated with Student t-test for statistical significance.Results:Results were calculated based on degree of hearing loss against PTA.FFCT‘s rate of detection of hearing loss was significant for all degrees of hearing loss with p-values of <0.05. FFVT’s detection rate was only significant to normal hearing, moderate & severe hearing loss. FFCT had higher sensitivity of 94.1% for normal hearing compared to 52% for FFVT.FFVT had higher specificity but with a marginal difference only.A higher rate of accuracy (94%) and strength of the correlation (0.97) were obtained for FFCT than FFVT.Conclusion:FFCT is a more sensitive&accurate tool than FFVT in detecting hearing thresholds. It proves to be an ideal tool to screen hearing loss and is convenient in times of social distancing during COVID-19 pandemic now.
Comparison of Robot-assisted Versus Conventional Laparoscope on Incidence and Risk Factors of Postoperative Delirium for Patients undergoing radical prostatectomy: A single-center Retrospective Analysis
Authors: Hu GuangJun , Weidong Shao , Mengda Xu , Bixi Li , Li Li , Bo Xu 
Published: October 2020

Abstract
Background: Robot-assisted laparoscopic radical prostatectomy ( RALRP) has better effects than conventional laparoscopic radical prostatectomy (LRP). However, there were few research focus on the incidence and risk factors of postoperative delirium for patients with different procedures. Methods: Eighty-one cases of RLRP patients (group RALRP) and 71 cases of LRP patients (group LRP) under general anesthesia were collected from anesthesia information system from June 2013 to December 2019. Comparison of the time of preparation period before surgery(Tp), time of operation(To), time of anesthesia maintenance (Ta), time of anesthesia recovery(time from cessation of intravenous anesthetic to endotracheal extubation, Tr), time of endotracheal extubation(time from endotracheal extubation to discharge from post-anesthesia care unit, Te), dosage of anesthetic (propofol, sufentanil, remifentanil), PaCO2, plasma lactate level and airway pressure at different points (before pneumoperitoneum, post-pneumoperitoneum, after loosening pneumoperitoneum), the incidences of postoperative delirium and utilization of mannitol, IAP, dosage of vasoactive agents(ephedrine, methoxamine and atropine), fluid infusion volume, blood transfusion volume, blood loss, postoperative hospitalization time (Th) and overall cost. Results: The incidences of postoperative delirium and utilization of mannitol in group RALRP and LRP were (17.3% VS 32.4%, P=0.03;7.4% VS 21.1%, P=0.014), Tr was (31.52±9.89 VS 38.65±16.32 min; P=0.01,95%CI 2.72-11.54), IAP was (12.72±1.87 VS 13.37±1.12 mmHg; P=0.01, 95%CI 0.16-1.14), PaCO2 at post-pneumoperitoneum and after loosening pneumoperitoneum were (47.89±10.89 VS 38.65±16.32 mmHg; P <0.001, 95%CI 6.47-11.98; 40.77±5.34 VS 43.57±4.54 mmHg; P=0.001, 95%CI 1.17-4.37), the difference was statistically significant. Tp, To, Ta and overall cost of patients in the RLRP group were significantly longer than those in the LRP group (P <0.02). No statistical difference was found in other observation variables. The incidence of postoperative delirium was considered as the dependent variable for binary logistic regression analysis. The result showed the incidence of postoperative delirium increased 1.17 times (95%CI 1.06-1.17), 1.13 times (95%CI 1.03-1.24), and 1.66 times (95%CI 1.02-2.70) for each unit increase in Tr, post-pneumoperitoneum PaCO2 and IAP.Conclusion: Compared with group LRP, patients in group RALRP had lower IAP, lower incidence of postoperative delirium and shorter recovery time, but the overall cost was higher. IAP is the main factor affecting the incidence of postoperative delirium.
Prevalence of Lead Toxicity in Adolescents in Kuwait
Authors: Jallad Reem , Rao Muddanna , Rahman Abdur 
Published: October 2020

Abstract
Background: Environmental lead (Pb) exposure is a public health problem in many developing and industrialized countries. Being a petrochemical industry-based economy, Pb levels are expected to be high in Kuwait but systematic data on population exposure are lacking. This study aimed at determining the prevalence of Pb toxicity in adolescents in Kuwait.Methods: Adolescents (N=1385; age range 11-16 years) were cross-sectionally selected from public middle schools from all Governorates of Kuwait, utilizing multi-stage cluster random sampling. Pb in whole blood was analyzed by inductively coupled plasma mass spectrometry. Pb levels among Governorates and genders were compared by median test and the prevalence of Pb levels above the CDC cutoff (≥5 µg/dL) was estimated by x2 test. Multiple logistic regression was used for association between prevalence of high Pb levels and Governorate.Results: Median (IQR) Pb was 5.1(3.6 – 7.1) µg/dL [4.9 (3.8 6.5) µg/dL in males and 5.4 (3.3-7.6) µg/dL in females; p=0.001]. In the overall sample, 51% had Pb levels ≥5 µg/dL; 13% were ≥10 µg/dL and 3% >20 µg/dL. Prevalence of Pb ≥5 µg/dL was 47% in males and 56% in females (p<0.001). High Pb levels were clustered in Al-Asima and Al-Ahmadi (in both genders); Al-Jahra (in males) and Mubarak Al-Kabeer (in females) Governorates.Conclusion: Pb exposure is a significant public health problem in adolescents in Kuwait. Urgent public health intervention is required in high Pb exposure areas and further research is needed to identify the sources of exposure in these areas for prevention.