bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary Protein on Growth Performance, Serum Biochemical Index and offspring of Hu ewes in late pregnancy
Authors: Wang Xin , Wang Qiye , Wang Yancan , Dai Chunpeng , Li Jianzhong , Huang Pengfei , Li Yali , Ding Xueqin , Huang Jing , Hussain Tarique , Yang Huansheng 
Published: August 2020

ABSTRACTThe purpose of this study was to explore the effects of ewe growth performance, serum biochemical indicators and lamb growth and development at different protein levels in late pregnancy. A total of 15 pregnant ewes (46.4 ± 1.38kg initial BW) were assigned to 3 groups with 5 ewes in each group in a randomized block design. P1, P2 and P3 of three groups were fed diets with different levels of crude protein at 11.25%, 12.5% and 13.75% (low, medium and high) respectively, while the dietary energy levels remained unchanged. Choose ewes with the same body condition from 90 days of pregnancy to different protein diets during delivery, and feed the same diets during lactation. New-born lambs were left to suckle their dams freely for the first three days and were kept with their dams till weaning at 60 days of age. Dietary protein levels during late gestation showed no significant effect on the weight gain of ewes and their offspring in the late pregnancy (P> 0.05) but had significant effects on blood urea nitrogen (BUN), glucose (GLU), C-reactive protein (CRPL3) and ammonia nitrogen (NH3L) before parturition, and on triglyceride (TG) in lactating ewes. Dietary protein levels during late gestation had significant effects on birth height, body length, chest depth, chest circumference, straight crown hip length and curved crown hip length of lambs (P< 0.05) as well as on weaning body length, chest circumference, head width and head length (P< 0.05). The results showed that although different dietary protein levels during late gestation have no significant effects on growth performance of ewes, singleton, twins and triplets, it affects lambs’ body shape and ewes’ metabolism. Therefore, the optimum protein concentration for the growth of ewes and subsequent lambs in this experiment is 11.25%.
Transcriptional regulation of ACSL1 by CHREBP and NF-kappa B in macrophages during hyperglycemia and inflammation
Authors: Thevkar-Nagesh Prashanth , Rawal Shruti , Zahr Tarik , Fisher Edward A. , Garabedian Michael J. 
Published: August 2020

ABSTRACTAcyl-CoA synthetase 1 (ACSL1) is an enzyme that converts fatty acids to acyl-CoA-derivatives for use in both lipid catabolism and lipid synthesis, including of arachidonic acid mediators that promote inflammation. ACSL1 has also been linked to the pro-atherosclerotic effects of diabetes in mice. ACSL1 expression has been reported to be upregulated in monocytes and macrophages by hyperglycemia, as well as enhanced by inflammatory stimuli, yet surprisingly little is known about the mechanisms underlying its transcriptional regulation. Here we show that increased Acsl1 mRNA expression in mouse macrophages by hyperglycemia is via transcription initiation such that nascent ACSL1 RNA and Acsl1 promoter activity are increased. We further demonstrate that the hyperglycemic-dependent induction of Acsl1 mRNA is governed by the glucose-sensing transcription factor, Carbohydrate Response Element Binding Protein (CHREBP), since the hyperglycemic upregulation of Acsl1 mRNA is lost in mouse bone marrow derived macrophages (BMDMs) from Chrebp knock out mice. In addition, we show that LPS treatment of mouse BMDMs increased Acsl1 mRNA, and this is attenuated by an NF-kappa B inhibitor that blocks p65 subunit binding to DNA. We further show that LPS treatment increased ACSL1 protein abundance and stimulated ACSL1 protein localization to membranes where it likely exerts its activity. Using an ACSL1 reporter gene containing the promoter and 1.6 Kb of upstream regulatory region, which contain multiple predicted CHREBP and NF-kappa B (RELA) binding sites conserved between the human and mouse ACSL1 gene, we found a synergistic increase of ACSL1 promoter activity when CHREBP and RELA were co-expressed. Thus, we have identified pathways controlling the expression of ACSL1 by hyperglycemia and inflammation through CHREBP and NF-kappa B.
Transmission and antibiotic resistance ofAchromobacterin cystic fibrosis
Authors: Gabrielaite Migle , Bartell Jennifer A. , Nørskov-Lauritsen Niels , Pressler Tacjana , Nielsen Finn C. , Johansen Helle K. , Marvig Rasmus L. 
Published: August 2020

AbstractAchromobacterspecies are increasingly being detected in patients with cystic fibrosis (CF), and this emerging pathogen is associated with antibiotic resistance and more severe disease outcomes. Nonetheless, little is known about the extent of transmission and antibiotic resistance development inAchromobacterinfections.We sequenced the genomes of 101 clinical isolates ofAchromobacter(A. xylosoxidansbased on MALDI-TOF/API N20 typing) collected from 51 patients with CF—the largest longitudinal dataset to-date. We performed phylogenetic analysis on the genomes and combined this with epidemiological and antibiotic resistance data to identify patient-to-patient transmission and development of antibiotic resistance.We found that MALDI-TOF/API N20 was not sufficient forAchromobacterspecies-level typing, and that the population ofAchromobacterisolates was composed of five different species whereA. xylosoxidansaccounted for 52% of infections. Most patients were infected by uniqueAchromobacterclone types; nonetheless, suspected patient-to-patient transmission cases identified by shared clone types were observed in 35% (N=18) of patients. In 15 of 16 cases the suspected transmissions were further supported by genome- or clinic visit-based epidemiological analysis. Finally, we found that resistance developed over time.We show that whole-genome sequencing (WGS) is essential forAchromobacterspecies typing and patient-to-patient transmission identification which was identified inA. ruhlandii, A. xylosoxidansand, for the first time,A. insuavis. Furthermore, we show that the development of antibiotic resistance is associated with chronicAchromobacterinfections. Our findings emphasize that transmission and antibiotic resistance should be considered in future treatment strategies.
Mosquito indolergic receptors belong to an ancient and functionally conserved Dipteran gene lineage
Authors: Pitts R. Jason , Shih Shan Ju , Bohbot Jonathan D. 
Published: August 2020

AbstractDiptera is a megadiverse group of flies with sophisticated chemical detection systems, which exploits an incredible variety of ecological niches. Among the vast array of odorants in natural environments, indoles stand out as playing crucial roles in mediating fly behavior. In mosquitoes, indolic compounds are detected by an ancient class of conserved indolergic Odorant Receptors (indolORs). In this study, we have identified a set of 92 putativeindolORgenes encoded in the genomes of Nematoceran and Brachyceran flies, resolved their phylogenetic relationships, and defined conserved elements in their gene structures. Further, we have quantifiedindolORtranscript abundance in the antennae of the housefly,Musca domestica, and have characterized MdomOR30a as a skatole receptor using a heterologous expression system. The presence ofindolORsin species operating in different ecological contexts suggests that indoles act as pleiotropic signals for resource exploitation at multiple developmental stages. Further characterization ofindolORswill impact our understanding of insect chemical ecology and will provide targets for the development of novel odor-based tools that can be integrated into existing vector surveillance and control programs.
Lymphopenia-induced T cell proliferation is a hallmark of severe COVID-19
Authors: Adamo Sarah , Chevrier Stéphane , Cervia Carlo , Zurbuchen Yves , Raeber Miro E. , Yang Liliane , Sivapatham Sujana , Jacobs Andrea , Bächli Esther , Rudiger Alain , Stüssi-Helbling Melina , Huber Lars C. , Schaer Dominik J. , Bodenmiller Bernd , Boyman Onur , Nilsson Jakob 
Published: August 2020

AbstractCoronavirus disease 2019 (COVID-19), caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has a broad clinical presentation ranging from asymptomatic infection to fatal disease. Different features associated with the immune response to SARS-CoV-2, such as hyperinflammation and reduction of peripheral CD8+T cell counts are strongly associated with severe disease. Here, we confirm the reduction in peripheral CD8+T cells both in relative and absolute terms and identify T cell apoptosis and migration into inflamed tissues as possible mechanisms driving peripheral T cell lymphopenia. Furthermore, we find evidence of elevated serum interleukin-7, thus indicating systemic T cell paucity and signs of increased T cell proliferation in patients with severe lymphopenia. Following T cell lymphopenia in our pseudo-longitudinal time course, we observed expansion and recovery of poly-specific antiviral T cells, thus arguing for lymphopenia-induced T cell proliferation. In summary, this study suggests that extensive T cell loss and subsequent T cell proliferation are characteristic of severe COVID-19.
Integrative Responses of Leaf-Cutting Ants to Temperature Rises
Authors: de Sousa Lima Cleverson , Helene André Frazão , Camacho Agustín 
Published: August 2020

AbstractThermal variation has complex effects on organisms and they deal with it by combining behavioral and physiological thermal tolerance. However, we still do not understand well how these two types of traits relate to body condition (e.g. size, hydration) and environmental variables (e.g. relative humidity), some of which are typical aspects of thermal tolerance experiments (warming rates, start temperature). We explored these interactions using a set of experiments that sequentially measure behavioral (Voluntary Thermal Maxima) and physiological thermal tolerance (Critical Thermal Maxima) for individuals ofAtta sexdens rubropilosa(Forel, 1908). We found non-linear effects of body size on behavioral thermal tolerance and refuted the traditional hypothesis that body size increases ant’s physiological thermal tolerance. Hydration state and humidity had complex effects on behavioral and physiological tolerance. However, both tolerance measures increased with heating rates and start temperature. Our work helps understanding how an ectotherm integrates stimuli affecting its thermal tolerance to decide which temperatures to avoid. We discuss implications for the ecology of ants, their labor division, and for their susceptibility to climate warming and drought.Summary StatementHere we show how internal (body size, hydration level) and external factors (heating rate, relative humidity) affect leaf-cutting ants behavioral and physiological responses to temperature rises.
Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysis
Authors: Bull Rowena A. , Adikari Thiruni , Ferguson James M. , Hammond Jillian M. , Stevanovski Igor , Beukers Alicia G. , Naing Zin , Yeang Malinna , Verich Andrey , Gamaarachchi Hasindu , Kim Ki Wook , Luciani Fabio , Stelzer-Braid Sacha , Eden John-Sebastian , Rawlinson William D. , van Hal Sebastiaan J. , Deveson Ira W. 
Published: August 2020

ABSTRACTViral whole-genome sequencing (WGS) provides critical insight into the transmission and evolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Long-read sequencing devices from Oxford Nanopore Technologies (ONT) promise significant improvements in turnaround time, portability and cost, compared to established short-read sequencing platforms for viral WGS (e.g., Illumina). However, adoption of ONT sequencing for SARS-CoV-2 surveillance has been limited due to common concerns around sequencing accuracy. To address this, we performed viral WGS with ONT and Illumina platforms on 157 matched SARS-CoV-2-positive patient specimens and synthetic RNA controls, enabling rigorous evaluation of analytical performance. Despite the elevated error rates observed in ONT sequencing reads, highly accurate consensus-level sequence determination was achieved, with single nucleotide variants (SNVs) detected at >99% sensitivity and >99% precision above a minimum~60-fold coverage depth, thereby ensuring suitability for SARS-CoV-2 genome analysis. ONT sequencing also identified a surprising diversity of structural variation within SARS-CoV-2 specimens that were supported by evidence from short-read sequencing on matched samples. However, ONT sequencing failed to accurately detect short indels and variants at low read-count frequencies. This systematic evaluation of analytical performance for SARS-CoV-2 WGS will facilitate widespread adoption of ONT sequencing within local, national and international COVID-19 public health initiatives.
Interaction of clinical-stage antibodies with heme predicts their physiochemical and binding qualities
Authors: Lecerf Maxime , Kanyavuz Alexia , Rossini Sofia , Dimitrov Jordan D. 
Published: August 2020

AbstractImmunoglobulin repertoires contain a fraction of antibodies that recognize low molecular weight compounds, including some enzymes’ cofactors, such as heme. Here, by using a set of 113 samples with variable region sequences matching clinical-stage antibodies, we demonstrated that a considerable number of these antibodies interact with heme. Antibodies that interact with heme possess specific sequence traits of their antigen-binding regions. Moreover they manifest particular physicochemical and functional qualities i.e. increased hydrophobicity, higher propensity of self-binding, higher intrinsic polyreactivity and reduced expression yields. Thus, interaction with heme is a strong predictor of different molecular and functional qualities of antibodies. Notably, these qualities are of high importance for therapeutic antibodies, as their presence was associated with failure of drug candidates to reach clinic. Our study reveled an important facet of information about relationship sequence-function in antibodies. It also offers a convenient tool for detection of liabilities of therapeutic antibodies.
BioMAX – the first macromolecular crystallography beamline at MAX IV Laboratory
Authors: Ursby Thomas , Åhnberg Karl , Appio Roberto , Aurelius Oskar , Barczyk Artur , Bartalesi Antonio , Bjelčić Monika , Bolmsten Fredrik , Cerenius Yngve , Doak R. Bruce , Eguiraun Mikel , Eriksson Thomas , Friel Ross J. , Gorgisyan Ishkhan , Gross Andrea , Haghighat Vahid , Hennies Franz , Jagudin Elmir , Norsk Jensen Brian , Jeppsson Tobias , Kloos Marco , Lidon-Simon Julio , de Lima Gustavo M. A. , Lizatovic Robert , Lundin Magnus , Milan-Otero Antonio , Milas Mirko , Nan Jie , Nardella Alberto , Rosborg Anders , Shilova Anastasya , Shoeman Robert L. , Siewert Frank , Sondhauss Peter , Talibov Vladimir O. , Tarawneh Hamed , Thånell Johan , Thunnissen Marjolein , Unge Johan , Ward Christopher , Gonzalez Ana , Mueller Uwe 
Published: August 2020

BioMAX is the first macromolecular crystallography beamline at the MAX IV Laboratory 3 GeV storage ring, which is the first operational multi-bend achromat storage ring. Due to the low-emittance storage ring, BioMAX has a parallel, high-intensity X-ray beam, even when focused down to 20 µm × 5 µm using the bendable focusing mirrors. The beam is tunable in the energy range 5–25 keV using the in-vacuum undulator and the horizontally deflecting double-crystal monochromator. BioMAX is equipped with an MD3 diffractometer, an ISARA high-capacity sample changer and an EIGER 16M hybrid pixel detector. Data collection at BioMAX is controlled using the newly developed MXCuBE3 graphical user interface, and sample tracking is handled by ISPyB. The computing infrastructure includes data storage and processing both at MAX IV and the Lund University supercomputing center LUNARC. With state-of-the-art instrumentation, a high degree of automation, a user-friendly control system interface and remote operation, BioMAX provides an excellent facility for most macromolecular crystallography experiments. Serial crystallography using either a high-viscosity extruder injector or the MD3 as a fixed-target scanner is already implemented. The serial crystallography activities at MAX IV Laboratory will be further developed at the microfocus beamline MicroMAX, when it comes into operation in 2022. MicroMAX will have a 1 µm × 1 µm beam focus and a flux up to 1015 photons s−1 with main applications in serial crystallography, room-temperature structure determinations and time-resolved experiments.
Factors influencing trust and behavioral intention to use Airbnb service innovation in three ASEAN countries
Authors: Chua Evelyn Lim , Chiu Jason Lim , Chiu Candy Lim 
Published: August 2020

Purpose
The sharing economy is described as a community marketplace, particularly home sharing such as Airbnb, which is more prevalent. Airbnb changed the way renters and tourists find places to stay when they are traveling. The company introduced innovations in business models and technologies. So, Airbnb requires specific factors that will influence consumers’ trust because consumers intuitively seek out trusting factors to make judgments on innovative service providers. Thus, the purpose of this study is to understand the factors that influence travelers’ trust to use Airbnb within the three ASEAN nations.

Design/methodology/approach
The data were collected from both qualitative and quantitative methods. The questionnaire was the main data-gathering instrument used in this study and supplemented by informal interviews. A self-administered questionnaire was provided to 130 Airbnb users from the Philippines, Indonesia and Singapore using Hayes’ Process Macro as the statistical tool.

Findings
The correlation test was carried out to determine the strength and relationships among the independent, mediating and dependent variables. All independent variables are positively correlated with the mediating variable. The results reveal that ease of use, convenience, information social influence, normative social influence and security have a significant impact on trust and behavioral intention to use Airbnb.

Originality/value
This study contributes to the field of sharing economy, particularly home sharing, by examining different factors that influence trust and behavioral intention. This study focused on the case of Southeast Asian consumers, so this study is useful for marketing practitioners to enhance their marketing strategies in catering to this segment of the market.
Hybrid cascode compensation with Q-factor control module for three-stage OTAs driving ultra-large load capacitors
Authors: Aminzadeh Hamed , Valinezhad Mohammad Mahdi 
Published: August 2020

Purpose
The purpose of this study is to discuss the effect of hybrid cascode compensation with quality factor (Q-factor) control module for the three-stage amplifiers driving ultra-large load capacitors. Compared to the present frequency compensation solutions, it extends the amplifier bandwidth by establishing an extra AC feedback pathway besides the primary pathway through the Miller capacitor, increasing the loop gain at the gain–bandwidth product (GBW) frequency by pushing to the higher frequencies the nondominant poles.

Design/methodology/approach
A Q-factor control block is used to improve the damping factor of the compensation loop with no power or area overhead, thereby reducing the frequency peaking and the undesired oscillation in the time response for small load capacitors. The Q-factor control module is realized by a tiny-size on-chip capacitor, and provides an extra feedback loop to feed the damping current back to the input stage. A left-half-plane (LHP) zero is also introduced to further improve the stability.

Findings
A prototype of the proposed amplifier is simulated in 180-nm CMOS with a quiescent current of 24-µA from 1.80-V voltage supply. It achieves a 3.98-MHz gain–bandwidth product for 500-pF load capacitor, while the overall compensation capacitor is limited to 0.5-pF and the DC gain is extended beyond 100-dB.

Originality/value
The proposed amplifier is absolutely stable for the load capacitors ranging between 80-pF and 100-nF.
International marketing doctrine: the use of guiding principles
Authors: Asseraf Yoel , Gnizy Itzhak , Shoham Aviv 
Published: August 2020

PurposeMarketing doctrine (MD) refers to a “firm's unique principles, distilled from its experiences, which provide firm-wide guidance on market-facing choices” (Challagalla et al., 2014, p. 4). Drawing on the knowledge-based view, the purpose of this paper is to develop a model of how MD is used and provide the first quantitative test of its relationship with business success.Design/methodology/approachThe authors advance the understanding of MD by providing a mixed-methods paper. In Study 1, a survey-based quantitative study was used. The final sample comprised 349 internationally active strategic business units (SBUs) of Israeli firms. Data were analysed using structural equation modelling. Study 2 provides insights into the use of MD based on 20 in-depth interviews.FindingsThe cross-sectional evidence shows that there tends to be more MD Use in higher-performing firms. The important roles of MD Clarity and MD Knowledgeability as mobilising processes of MD Use are demonstrated. Learning by doing impacts MD Use only through MD Clarity and MD Knowledgeability.Practical implicationsMD is a new strategic tool that can be applied practically. MD may provide a straightforward way of communication between international ventures. MD Use may allow global consistency and flexibility within local markets, simultaneously. Therefore, marketing managers are advised to supplement MD to their portfolio of management tools.Originality/valueThis paper is the first to investigate empirically, through newly developed scales, whether and how MD's core processes (learning by doing, MD Clarity, MD Knowledgeability and MD Use) are related to the success of international ventures.
Factors affecting BIM application in China: a social network model
Authors: Tai Shuangliang , Zhang Yao , Li Ting 
Published: August 2020

Purpose
The purpose of this paper is to promote the application of building information modeling (BIM) in China’s construction industry, key factors and their relationships are explored.

Design/methodology/approach
Based on a literature review, 28 factors were extracted and their relationships (239 in total) obtained using the Delphi method. A social network model of the factors was constructed and factors were analyzed using social network analysis (SNA).

Findings
The top 10 key factors and their relationships were obtained using SNA. Among the top 10 critical factors, six were source factors. They were: training for the application of BIM, guidance from experts, proper management modes, efficient BIM teams, specifications and demonstrations and standards for building information communication. The other four factors included as follows: a willingness to accept BIM, knowledge of its value and benefits, the definition of its benefits and the availability of IT software and hardware. These were mediating factors that could further the influence of the source factors.

Originality/value
The results provide useful information for public agencies and professionals to understand the immediate and mediating influences of the factors on the application of BIM. Solutions and future efforts for different participants are presented to promote the application of BIM-based on the key factors and their relationships.