bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of Aurora kinase signaling allows lung cancer cells to adopt an alternative cell cycle and form multinucleated polyploid giant cells that resist anti-mitotic drugs
Authors: Tagal Vural , Roth Michael G. 
Published: December 2019

AbstractPolyploid multinucleated (PP/MN) giant cancer cells are common in tumors and have been associated with resistance to cancer therapy, tumor relapse, malignancy, immunosuppression, metastasis, cancer stem cell production and modulation of the tumor microenvironment. However, the molecular mechanisms that cause these cells to form are yet known. In this study, we discover that Aurora kinases are synergistic determinants of a switch from the proliferative cell cycle to polyploid growth and multinucleation in lung cancer cell lines. When Aurora kinases are inhibited together, lung cancer cells uniformly grow into PP/MN giant cells. These cells have adopted an endocycle in which the genome replicates, mitosis is omitted and cells grow in size. Consequently, such cells continue to safely grow in the presence of antimitotic agents. These polyploid multinucleated cancer cells can re-enter the proliferative cell cycle and grow in cell number when the treatment is terminated.
Characterization of the impact of daclizumab beta on circulating natural killer cells by mass cytometry
Authors: Ranganath Thanmayi , Simpson Laura J. , Seiler Christof , Ferreira Anne-Maud , Vendrame Elena , Zhao Nancy , Fontenot Jason D. , Holmes Susan , Blish Catherine A. 
Published: December 2019

AbstractDaclizumab beta is a humanized monoclonal antibody that binds to CD25 and selectively inhibits high-affinity IL-2 receptor signaling. As a former treatment for relapsing forms of multiple sclerosis (RMS), daclizumab beta induces robust expansion of the CD56brightsubpopulation of NK cells that is correlated with the drug’s therapeutic effects. As NK cells represent a heterogeneous population of lymphocytes with a range of phenotypes and functions, the goal of this study was to better understand how daclizumab beta altered the NK cell repertoire to provide further insight into the possible mechanism(s) of action in RMS. We used mass cytometry to evaluate expression patterns of NK cell markers and provide a comprehensive assessment of the NK cell repertoire in individuals with RMS treated with daclizumab beta or placebo over the course of one year. Treatment with daclizumab beta significantly altered the NK cell repertoire compared to placebo treatment. As previously reported, daclizumab beta significantly increased expression of CD56 on total NK cells. Within the CD56brightNK cells, treatment was associated with multiple phenotypic changes, including increased expression of NKG2A and NKp44, and diminished expression of CD244, CD57, and NKp46. While the changes were less dramatic, CD56dimNK cells responded distinctly to daclizumab beta treatment, with higher expression of CD2 and NKG2A, and lower expression of FAS-L, HLA-DR, NTB-A, NKp30, and Perforin. Together, these data indicate that the expanded NK cells share features of both immature and mature NK cells. These findings show that daclizumab beta treatment is associated with unique changes in NK cells that may enhance their ability to kill autoreactive T cells or to exert immunomodulatory functions.
Determination of secretory granule maturation times in pancreatic islet beta-cells by serial block face scanning electron microscopy
Authors: Rao A. , McBride E.L. , Zhang G. , Xu H. , Cai T. , Notkins A.L. , Aronova M.A. , Leapman R.D. 
Published: December 2019

AbstractIt is shown how serial block-face electron microscopy (SBEM) of insulin-secreting beta cells in wild-type mouse pancreatic islets of Langerhans can be used to determine maturation times of secretory granules. Although SBEM captures the beta cell structure at a snapshot in time, the observed ultrastructure can be considered representative of a dynamic equilibrium state of the cells since the pancreatic islets are maintained in culture in approximate homeostasis. It is found that 7.2±1.2% (±st. dev.) of the beta cell volume is composed of secretory granule dense-cores exhibiting angular shapes surrounded by wide (typically ≳100 nm) electron-lucent halos. These organelles are identified as mature granules that store insulin for regulated release through the plasma membrane, with a release time of 96±12 hours, as previously obtained from pulsed 35S-radiolabeling of cysteine and methionine. Analysis of beta cell 3D volumes reveals a subpopulation of secretory organelles without electron-lucent halos, identified as immature secretory granules. Another subpopulation of secretory granules is found with thin (typically ≲30 nm) electron-lucent halos, which are attributed to immature granules that are transforming from proinsulin to insulin by action of prohormone convertases. From the volume ratio of proinsulin in the immature granules to insulin in the mature granules, we estimate that the newly formed immature granules remain in morphologically-defined immature states for an average time of 135±14 minutes, and the immature transforming granules for an average time of 130±17 minutes.
Chromatin-accessibility estimation from single-cell ATAC data with scOpen
Authors: Li Zhijian , Kuppe Christoph , Ziegler Susanne , Cheng Mingbo , Kabgani Nazanin , Menzel Sylvia , Zenke Martin , Kramann Rafael , Costa Ivan G. 
Published: December 2019

AbstractA major drawback of single cell ATAC (scATAC) is its sparsity, i.e. open chromatin regions with no reads due to loss of DNA material during the scATAC-seq protocol. We propose scOpen, a computational method for imputing and quantifying the open chromatin status of regulatory regions from sparse scATAC-seq experiments. We show that scOpen improves crucial down-stream analysis steps of scATAC-seq data as clustering, visualisation, cis-regulatory DNA interactions and delineation of regulatory features. We demonstrate the power of scOpen to dissect regulatory changes in the development of fibrosis in the kidney. This identified a novel role of Runx1 and target genes by promoting fibroblast to myofibroblast differentiation driving kidney fibrosis.
Sensing Traction Force on Matrix Induces Cell-Cell Distant Mechanical Communications for Self-assembly
Authors: Ouyang Mingxing , Qian Zhili , Bu Bing , Jin Yang , Wang Jiajia , Liu Lei , Pan Yan , Deng Linhong 
Published: December 2019

AbstractThe long-range biomechanical force propagating across large scale may reserve the capability to trigger coordinative responses within cell population such as during angiogenesis, epithelial tubulogenesis, and cancer metastasis. How cells communicate in a distant manner within the group for self-assembly remains largely unknown. Here we found that airway smooth muscle cells (ASMCs) rapidly self-assembled into well-constructed network on 3D Matrigel containing type I collagen (COL), which relied on long-range biomechanical force across the matrix to direct cell-cell distant interactions. Similar results happened by HUVEC cells to mimic angiogenesis. Interestingly, single ASMCs initiated multiple extended protrusions precisely pointing to neighboring cells in distance, depending on traction force sensing. Separate ASMCs sensed each other to move directionally on both non-fibrous Matrigel and more efficiently when containing fibrous COL, but lost mutual sensing on fixed gel or coated glass due to no long-range force transmission. Beads tracking assay demonstrated distant transmission of traction force, and finite element method modeling confirmed the consistency between maximum strain distribution on matrix and cell directional movements in experiments. Furthermore, ASMCs recruited COL from the hydrogel to build fibrous network to mechanically stabilize cell network. Our results revealed for the first time that cells can sense traction force transmitted through the matrix to initiate cell-cell distant mechanical communications, resulting in cell directional migration and coordinative self-assembly with active matrix remodeling. As an interesting phenomenon, cells sound able to ‘make phone call’ via long-range biomechanics, which implicates physiological importance such as for tissue pattern formation.
Towards the “Homo Deus” excellence perspective?
Authors: Anninos Loukas N. 
Published: December 2019

Purpose
The evolution of management underlines the importance of the human, systemic, technological and contingency element and their interaction along with an amplified awareness of organizations for achieving excellence. This paper aims to discuss whether the fusion of digital, biological and physical world leads to a new excellence perspective and to investigate the potential value of informative neuroscientific findings for setting the foundations for smart services.

Design/methodology/approach
This study is based on a literature review regarding the advances of neurosciences and its implications for business. Their usefulness and potential contribution for the provision of smart services are investigated.

Findings
The fusion of technological evolution and biological sciences may potentially give birth to a new excellence conceptualization complemented by genetic data whose consequences are hard to predict. Neurosciences offer insights for various human behavior areas that can be used by business practitioners, to refine their thinking and management style and build brain-friendly organizational contexts. The combination of using neuroscientific evidence and technology in service systems sets the foundations for an “intelligent” provision of services in a quality context.

Originality/value
The paper investigates the conceptual development of excellence within the receding context of the “smart era” and the potential contribution of neurosciences for the provision of smart services with reference to quality pioneers’ theories and ideas.
Universities as the engine of transformational sustainability toward delivering the sustainable development goals
Authors: Purcell Wendy Maria , Henriksen Heather , Spengler John D. 
Published: December 2019

Purpose
Universities can do more to deliver against the sustainable development goals (SDGs), working with faculty, staff and students, as well as their wider stakeholder community and alumni body. They play a critical role in helping shape new ways for the world, educating global citizens and delivering knowledge and innovation into society. Universities can be engines of societal transformation. Using a multiple case study approach, this study aims to explore different ways of strategizing sustainability toward delivering the SDGs are explored in a university setting with an example from the UK, Bulgaria (Europe) and USA.

Design/methodology/approach
The first case is a public UK university that adopted enterprise and sustainability as its academic mission to secure differentiation in a disrupted and increasingly marketized global higher education sector; this became a source of inspiration for change in regional businesses and the local community. The second case is a business sector-led sustainability-driven transformation working with a private university in Bulgaria to catalyze economic regeneration and social innovation. Finally, a case from the office for sustainability in a major US research university is given to show how its engagement program connected faculty and students in sustainability projects within the institution and with external partners.

Findings
Each case is in effect a “living lab,” positioning sustainability as an intentional and aspirational strategy with sustainable development and the SDG framework a means to that end. Leadership at all levels, and by students, was key to success in acting with a shared purpose. Partnerships within and with universities can help accelerate delivery of the SDGs, enabling higher education to make a fuller contribution to sustaining the economic, environmental, cultural and intellectual well-being of our global communities.

Originality/value
The role of universities as the engine of transformational sustainability toward delivering the SDGs has been explored by way of three case studies that highlight different means toward that end. The collegiate nature of the higher education sector, with its shared governance models and different constituencies and performance drivers, means that sustainability at a strategic level must be led with leaders at all levels acting with purpose. The “living lab” model can become a part of transformative institutional change that draws on both top-down and bottom-up strategies in pursuit of sustainable development.
Have accounting measures lost their usefulness after the 2008 global financial crisis?
Authors: Almujamed Hesham I. , Alfraih Mishari M. 
Published: December 2019

Purpose
This purpose of this paper is to investigate the value relevance and incremental importance of earnings and book value in the Kuwaiti market to equity holders over time and in the context of the decade after the 2008 global financial crisis.

Design/methodology/approach
Following reports in the literature, the value relevance of earnings and book values was examined using the price valuation model provided by Ohlson (1995). Observations (2,817) were collected from all firms listed on the Kuwait Stock Exchange from 1994 to 2016.

Findings
The results suggest that the value relevance of earnings and book values declined over this period, and that the loss of value relevance for earnings data was greater than that for book value. The analysis provides evidence that the decline in value relevance of earnings and book value was driven by book values in the post-GFC period and suggests an exchange of value relevance between earning and book value post GFC.

Practical implications
The results are useful for regulators, analysts, investors and academics as an assessment of effectiveness of current financial reporting. There is a need for improvement because quality information helps equity holders determine value precisely. Timely financial reporting may mitigate the drop in value relevance of financial statements.

Originality/value
This is the first study to examine value relevance accounting measures of Kuwaiti companies, in the post-GFC context. It contributes to capital market research through an empirical examination of a frontier capital market.
State of the sector: US worker cooperatives in 2017
Authors: Palmer Tim 
Published: December 2019

Purpose
This is a national census of all worker cooperatives in the USA for 2017 implemented by the Democracy at Work Institute which is affiliated with the United States Federation of Worker Cooperatives, the largest worker cooperative association in the USA. The paper aims to discuss these issues.

Design/methodology/approach
Longitudinal survey with descriptive analysis of results.

Findings
The author identified 394 worker cooperatives and democratic workplaces in all areas of the country in fiscal year 2017 (though early tracking for 2018 shows at least 400 such businesses). While the typical worker cooperative is small (about nine workers and about $588,600 gross annual revenue) and either recently launched, or converted to the form, there are many notable examples of very large and/or long lasting businesses. Together they employ an estimated 6,734 workers and produce about $467m in revenue each year. The following analysis attempts to outline the basic geographic, structural, economic and demographic features of these enterprises.

Research limitations/implications
The author reached out to this list of businesses via e-mail and telephone contact between August and November 2018. This included both members of the US Federation of Worker Cooperatives and non-members. The author asked participants to provide information based on their fiscal year 2017 performance. The survey asked similar questions to the prior year’s survey (e.g. total revenue, total assets, total wages, net income, total workers, total worker–owners, etc.), but also included some small changes in content, format and style. In total, 105 worker cooperatives responded and either fully or substantially completed the survey questions. All non-responsive firms were verified to be in business and operate as worker cooperatives in 2017 to the best of the author’s knowledge. Regardless of whether they responded, the author assigned all businesses an industry (NAICS) classification, foundational year and primary location based on basic internet research. This allowed the author to examine the larger universe when analyzing issues around the geography, age and industry of these enterprises. Additionally, internet research also allowed the author to add some other information about a portion of the non-responding worker cooperatives (e.g. number of workers, etc.) when such information was self-reported on their websites.

Originality/value
This is the only census of worker cooperatives done in the USA to the author’s knowledge.
The role of teachers’ self-efficacy between trust and organisational citizenship behaviour among secondary school teachers
Authors: Choong Yuen Onn , Ng Lee Peng , Ai Na Seow , Tan Chun Eng 
Published: December 2019

PurposeThe purpose of this paper is to examine the effects of teachers’ perception on trust over their willingness to exercise organisational citizenship behaviours (OCB) using self-efficacy as a mediator.Design/methodology/approachUsable questionnaires were collected from 411 teachers in secondary schools. A two-stage analytic approach was used to analyse the data.FindingsThe results indicated that trust and teachers’ self-efficacy dimensions (general teaching and personal teaching) are positively related to OCB. Additionally, trust in the principal, colleagues and clients are predicted to have indirect influence on OCB through self-efficacy.Practical implicationsTeachers’ personal sense of efficacy is largely dependent on the amount of efforts devoted in their teaching, their decision-making ability and the degree of persistency in solving problematic issues. School management and policy makers are urged to develop effective human resources initiatives and programmes that can create a trusting relationship in the organisation and enhance teachers’ self-efficacy. These may include the socialisation programmes that can inculcate teachers’ inner natures, confidence and interpersonal skills when directed towards perceived abilities in given specific tasks and responsibilities to make a significant impact on OCB. Apart from this, the school administrator is advised to offer relevant training and workshops that able to enhance the efficacy level of teachers.Originality/valueThis study explored how teachers’ self-efficacy was related to trust and OCB. Teachers’ positive behaviours enable them to have greater belief in their capability of handling pressures and crises. The study contributes to the current body of literature and creates a comprehensive theoretical framework for teachers to be involved in OCB. Besides, these unique findings served as a reference to management of any school to better understand the importance of trust in the school and how it relates to teacher self-efficacy, which, in turn nurture the citizenship behaviour in workplace.
A pilot study to assess an in-process inspection method for small diameter holes produced by direct metal laser sintering
Authors: Deja Mariusz , Zielinski Dawid 
Published: December 2019

Purpose
The purpose of this study is to evaluate the geometric quality of small diameter holes in parts printed by direct metal laser sintering (DMLS) technology. An in-process optical inspection method is proposed and assessed during a pilot study. The influence of the theoretical hole diameter assumed in a computer-aided design (CAD) system and the sample thickness (hole length) on the hole clearance was analyzed.

Design/methodology/approach
The samples are made of two different materials: EOS MaragingSteel MS1 and aluminium alloy EOS Aluminium consisted of straight through holes of different diameters and lengths. Dimensional and shape accuracy of the holes were determined with the use of the image processing software and the computer analysis of two-dimensional (2-D) images. The definition of the equivalent hole diameter was proposed to calculate the hole clearance. Feret’s diameters were determined for the evaluation of the shape accuracy.

Findings
The dependency between the equivalent hole diameter and the theoretical diameter was approximated by the linear function for a specific sample thickness. Additionally, a general empirical model for determining the hole clearance was developed, allowing for calculating the equivalent hole diameter as a function of a sample thickness and a theoretical hole diameter.

Practical implications
Developed functions can be used by designers for a proper assignment of a hole diameter to achieve the required patency. The relevant procedures and macros based on proposed empirical models can be embedded in CAD systems to support the designing process.

Originality/value
The analysis of the geometric quality of the holes in parts printed by DMLS was based on the computer analysis of 2-D images. The proposed method of assessing the shape accuracy of straight through holes is relatively cheap, is widely available and can be applied to the features of other shapes produced by three-dimensional printing.
Study on the improvement of ultra-wideband localization accuracy in narrow and long space
Authors: Cao Bo , Wang Shibo , Ge Shirong , Liu Wanli , Wang Shijia , Yi Shixue 
Published: December 2019

Purpose
Wireless network localization technology is very popular in recent years and has attracted worldwide attention. The purpose of this paper is to improve the localization accuracy of ultra-wideband (UWB) with lower localization error taking into consideration the special real environment with the closed long and narrow space.

Design/methodology/approach
The principle of multidimensional scaling (MDS), particle swarm optimization (PSO) and Taylor series expansion algorithm (Taylor-D) were introduced. A novel positioning algorithm, MDS-PSO-Taylor was proposed to minimize the localization error. MDS-PSO algorithm provided a more accurate preliminary coordinate by applying the PSO algorithm so that the Taylor-D was used for further enhancing the localization accuracy.

Findings
Experimental results manifested that the proposed algorithm, providing small localization error value and higher positioning accuracy, can effectively reduce errors and achieve better performance in terms of the considerable improvement of localization accuracy.

Originality/value
The presented study with the real environment test attempts to demonstrate the proposed algorithm is hopeful to be applied to the underground environment for in the future.