bluuebunny/crossref_metadata_2025_split huggingface dataset:
113 Effects of aquatic conditioning in young horses. I. Markers of inflammation and cartilage metabolism
Authors: Silvers Brittany L , Leatherwood Jessica L , Nielsen Brian D , Arnold Carolyn E , Dominguez Brandon , Glass Kati , Huseman Chelsie , Bradbery Amanda N , Much Mattea L , Martinez Rafael E 
Published: November 2020

Abstract
Aquatic treadmills improve range of motion and increase muscular strength in mature horses; however, effects of buoyancy on inflammation and cartilage metabolism in young horses are not well investigated. Therefore, thirty Quarter Horse yearlings of similar breeding were stratified by age, BW, and sex and randomly assigned to one of three treatment groups during a 140-d trial to evaluate the influence of aquatic vs. dry exercise on joint inflammation and cartilage metabolism in young horses transitioning to an advanced workload. Treatment groups included non-exercise control (CON; n = 10), dry treadmill exercise (DRY; n = 10), or aquatic treadmill exercise (H2O; n = 10; water at 60% wither height). Animals were housed in individual stalls and allowed turnout for a minimum of 10 h/d. During Phase I, DRY and H2O walked on treadmills 30 min/d, 5 d/wk from d 0 to d 112. Phase II represented transition to an advanced workload 5d/wk for 28 d (Table 1). Every 28 d following exercise, synovial fluid samples were collected and analyzed for prostaglandin E2 (PGE2), collagenase cleavage neopeptide (C2C), collagenase of type I and type II collagen (C1,2C), and carboxypeptide of type II collagen (CPII) using commercial ELISA kits. All data were analyzed using PROC MIXED of SAS, including random effect of horse within treatment, and repeated effect of day. Baseline treatment differences were accounted for using a covariate structure. There were no treatment ′ day interactions of synovial inflammation or markers of cartilage metabolism; however, there was an effect of day for each selected marker (P &lt; 0.03). Changes in biomarkers of cartilage turnover in horses exercised at the walk, whether dry or aquatic, could not be distinguished from horses with access to turnout alone. This indicates that there are no negative effects of buoyancy on cartilage metabolism in yearlings transitioned from aquatic exercise to 28-d advanced workload.
105 Chromium propionate improves insulin sensitivity in horses following oral concentrate consumption
Authors: Spears Jerry W , Siciliano Paul , Pratt-Phillips Shannon , Lloyd Karen , Goertzen Ellen , Krafka Kristi , Hyda Jill , Rounds Whitney 
Published: November 2020

Abstract
Forty-eight Quarter Horse geldings (3–8 yr of age) were used to determine the effects of dietary chromium (Cr), in the form of Cr propionate (Cr Prop), on insulin sensitivity. Horses were blocked by age, body condition score, and glucose response to concentrate feeding on d 0, and randomly assigned to treatments. Treatments consisted of 0, 2, 4, or 8 mg Cr/d from Cr Prop. Horses were fed daily a concentrate mix at a rate of 0.2 kg/100 kg BW and grass hay at 1.75 to 2.0 kg/100 kg BW. After an overnight fast, jugular blood samples were obtained at 0, 2, and 4 h after concentrate feeding on d 0 and 28 for determination of glucose and insulin. Plasma glucose on d 28 was affected (P &lt; 0.05) by treatment, time, and treatment x time. Horses fed 4 mg Cr/d had lesser (P &lt; 0.05) plasma glucose concentrations than those in the other treatments at 0 h. At 2 h post feeding glucose concentrations were greater (P &lt; 0.05) in control horses than in those given 4 mg Cr. Horses fed 2 mg Cr/d had lesser (P &lt; 0.05) plasma glucose at 4 h post feeding compared to controls. Plasma glucose did not differ among horses receiving 2 or 4 mg Cr/d at 2 or 4 h. Insulin concentrations were greater (P &lt; 0.05) in horses fed 0 or 2 mg Cr/d than in those given 4 or 8 mg Cr at 0 h. At 4 h post feeding insulin concentrations were greater (P &lt; 0.05) in control horses than in those fed 2 or 4 mg Cr/d. Results of this study indicate that 2 or 4 mg Cr/d from Cr Prop increased insulin sensitivity in adult horses following oral carbohydrate consumption.
363 Meat Quality Matters: Trends and Mitigation Strategies for Muscle Myopathies in the Broiler Meat Industry
Authors: Owens Casey M , Jackson Ashunti Ria 
Published: November 2020

Abstract
Broiler breast myopathies, including woody breast (WB), white striping (WS), and spaghetti breast (SB), negatively impact the industry. Broiler breast myopathy trends show that myopathies generally increase as the birds get older and larger, and they also increase as breast yield increases to higher levels. Gender can have some impact on myopathies, as males tend to have a higher frequency of WB and females have a higher frequency of SB. Mitigation strategies in both plant and live production are needed. In the short term, it is possible to sort carcasses/fillets manually in processing plants and technology is being developed to detect these issues automatically. These online methods may include image analysis of carcass/fillet shape or dimension features, or evaluating compression force to determine hardness. Fillets determined to be severe for these defects may be diverted into products such as comminuted ones, that are less impacted by appearance or reduced functionality relative to whole fillets. In live production, evaluating strategies to slow down growth rate overall (e.g. management or nutrition), or at strategic times, may be worth investigating, though this would be a short-term mitigation strategy. In the long term, strategies should focus on genetic programs. Breeder companies have always been committed in producing exceptional products. A multitude of methods for early detection of myopathies have tested over the years. Currently, innovative techniques centered around machine learning and more structured breeding programs have been leading the way in further improving meat quality without sacrificing yield. These and other intriguing approaches centralizing on the live bird will drive a positive trend in the quality and quantity of protein produced in the broiler industry.
121 Effects of DON on the porcine growth performance and intestinal microbiota and potential remediation by a novel modified HSCAS adsorbent
Authors: Yang Jia-Cheng , Lei Zhang , Liu Shuai , Chu Xiao-Han , Zhang Ni-Ya , Sun Lv-hui 
Published: November 2020

Abstract
The objective of the current study was to evaluate the effects of deoxynivalenol (DON) on growth performance and intestinal microbiota in weaning piglets, and potential efficacy of a modified hydrated sodium calcium aluminosilicate (HSCAS) binder to detoxify DON toxicity. Four groups of 21-day-old male piglets (n = 7/group) were fed either a control diet, or diet adding 1.0 or 3.0 mg/kg DON, or 3.0 mg/kg DON plus 0.05% modified HSCAS for 28 d. Compared to the control, dietary DON at 1.0 and/or 3.0 mg/kg decreased (P &lt; 0.05) the feed intake (18.1–38.7%) and body weight gain (16.0–60.8%) during the whole experiment, and increased (P &lt; 0.05) the feed/gain ratio (12.8–33.8%) during d 1–14. Worse results were obtained at 3.0 mg/kg DON in comparison to 1.0 mg/kg for most of these variables. DON exposure reshaped gut microbial structure by drastically dysregulating the abundance of several bacterial phyla, families, and genera, such as dysbiosis of Actinobacteria, Cyanobacteria, Firmicutes, and Proteobacteria in small intestine. Notably, dietary modified HSCAS binder supplementation mitigated the negative effects of DON on growth performance of piglets and improved the intestinal flora disorder. Therefore, this study has revealed that the modified HSCAS binder can mitigate DON-induced adverse effects and could be used as a promising countermeasure for detoxifying DON toxicity.
148 Effects of managing mature beef bulls on divergent planes of nutrition on body composition and concentrations of hormones and metabolites
Authors: Dahlen Carl R , Underdahl Sarah R , Crouse Matthew S , McCarthy Kacie L , Kassetas Cierrah J , Baumgaertner Friederike , Kirsch James D , Dorsam Sheri T , Sedivec Kevin K , Ward Alison K , Caton Joel 
Published: November 2020

Abstract
Fifteen mature beef bulls (BW = 800.4 ± 17.4 kg) were used in a 112-d experiment to evaluate effects of divergent planes of nutrition on body composition and concentrations of hormones and metabolites. Bulls were ranked by BW and randomly assigned to one of two treatments; 1) managed on a positive plane of nutrition (POS, n = 8), or 2) managed on a negative plane of nutrition (NEG, n = 7). Bulls were fed a common diet with deliveries into Insentec feeders adjusted biweekly to achieve targeted weight loss or gain (~12.5% of original BW). Blood samples were collected on d 0, 56, and 112. By design, bull BW was influenced by a treatment × day interaction (P &lt; 0.0001), and POS bulls gained 1.27 ± 0.08 kg/d while NEG lost 0.93 ± 0.08 kg/d. Body condition score and scrotal circumference were similarly impacted by treatment × day interactions (P &lt; 0.0001), both starting similar among treatments, then greater for POS than NEG thereafter. To achieve targeted weight divergence POS bulls (13.8 ± 0.45 kg/d) ate more (P &lt; 0.0001) than NEG (5.1 ± 0.45 kg/d). Body composition was impacted (P ≤ 0.02), with rump fat, rib fat, loin muscle area, and intramuscular fat each increasing in POS and decreasing in NEG. Concentrations of NEFA in serum were greater in NEG bulls at d 56 and 112 compared with POS (treatment × day; P &lt; 0.0001), concentrations of glucose (treatment × day; P = 0.02) were greater for POS bulls, and concentrations of urea were not impacted (P = 0.60). Additionally, concentrations of T3 and T4 on d 112 were greater (P ≤ 0.004) for POS. Our model resulted in altered body composition and profiles of hormones and metabolites which could influence semen on functional, morphological, and molecular levels.
153 Do pre and postnatal nutrition interact to program reproductive phenotype in sexually mature heifers?
Authors: Maia Tatiane S , Guimarães Higor R , Franco Gessica A , Pohler Ky G , Cardoso Rodolfo C , Williams Gary L 
Published: November 2020

Abstract
Objectives were to test the hypothesis that nutritional extremes during pregnancy in the bovine female negatively affect phenotypic characteristics of the estrous cycle of sexually mature offspring, and to determine whether these effects interact with postnatal diets during the juvenile period. Beginning at 90 d of pregnancy, Brangus and Braford (n = 108) beef cows with a female fetus were fed to achieve body condition scores of 7.5–8 (H, obese), 5–5.5 (M, moderate) or 3–3.5 (L, very thin) by the start of the third trimester. Heifer offspring were weaned and fed to gain at either a high (H; 1 kg/d) or low (L; 0.5 kg/d) rate between 4 and 8 months of age, then fed a common growth diet until puberty. Estrous cycles of a subgroup (n = 53) were synchronized using 2 injections of prostaglandin F2α(PGF). Transrectal ultrasonography was employed during the follicular phase of two estrous cycles to evaluate antral follicle count(AFC), rate of growth and size of the ovulatory follicle, size of corpus luteum(CL), and endometrial thickness. Blood samples were collected daily for assay of plasma concentrations of progesterone(P4) and estradiol-17β(E2) by RIA. Preliminary data (n = 35) were analyzed using ANOVA and mixed model procedures. Least Squares Means estrous cycle length (20.9 ± 0.2 days), AFC (19.7 ± 1.0), follicular growth rate (0.81 ± 0.11), ovulatory follicle size (12.8 ± 0.2 mm), CL size (16.8 ± 0.6 mm), and endometrial thickness (14.6 ± 0.3 mm) did not differ among treatments. Heifers in postnatal H group tended (P &lt; 0.1) to have a greater area (arbitrary units) under the E2 curve (12.6 ± 0.6) than postnatal L (11.3 ± 0.5) during the follicular phase. Normalized AUC for P4 was greater (P &lt; 0.03) in L/H (56.94 ± 3.22) than both M/H (42.98 ± 3.61) and L/L (43.66 ± 3.22) groups. Despite marked pre- and postnatal nutritional contrasts, independent and interactive effects of nutritional treatments have been of minor significance or undetectable to date.
152 Impact of chorionic somatomammotropin RNA interference on uterine blood flow and placental glucose uptake in the absence of intrauterine growth restriction
Authors: Tanner Amelia R , Ali Asghar , Winger Quinton A , Rozance Paul J , Anthony Russell V 
Published: November 2020

Abstract
Chorionic somatomammotropin (CSH) is one of the most abundant hormones produced by the sheep placenta, yet the exact function of CSH has been elusive. Previously we reported the use of in vivo RNA interference (RNAi) to assess the impact of CSH deficiency on placental and fetal growth in sheep. Near-term (135 dGA), there are two distinct CSH RNAi phenotypes: 1) pregnancies with intrauterine growth restriction (IUGR), and 2) pregnancies with normal fetal and placental weights. This study describes physiological changes in the latter phenotype. To generate the CSH RNAi pregnancies, the trophectoderm of hatched blastocysts (9 dGA) were infected with lentiviral-constructs expressing either a scrambled control (NTS) or CSH-specific shRNA (CSH RNAi), prior to transfer into synchronized recipient ewes. At 120 dGA, 6 NTS and 6 CSH RNAi pregnancies were fitted with maternal and fetal catheters. Uterine and umbilical blood flows were measured utilizing the 3H2O transplacental diffusion technique at 132 dGA, and nutrient uptakes were calculated by the Fick principle. Resulting data were analyzed by Student’s t-test and significance was set at P ≤ 0.05. CSH RNAi tended (P ≤ 0.10) to reduce placentome weight with no effect on fetal weight. Absolute (ml/min) and relative (ml/min/kg fetus) uterine blood flows were reduced (P ≤ 0.05) in CSH RNAi pregnancies, but umbilical flows were not impacted. The uterine artery-to-vein glucose gradient (mmol/l) was significantly (P ≤ 0.05) increased, whereas the gradients for taurine and glycine were reduced (P ≤ 0.05). Uteroplacental glucose uptake (mmol/min/kg placenta) was increased 27% (P ≤ 0.05), whereas umbilical glucose uptake (mmol/min/kg fetus) was reduced 13%. This cohort demonstrates that even in the absence of IUGR, CSH deficiency has significant physiological ramifications, and the investigation of CSH RNAi pregnancies exhibiting both IUGR and non-IUGR phenotypes may help determine the direct effects of CSH and its potential impact on fetal programming. Supported by NIH R01 HD093701.
158 Provision of artificial shade before weaning influences performance of Angus and Brangus cow-calf pairs
Authors: da Silva Gleise Medeiros , Schulmeister Tessa M , Podversich Federico , Tarnonsky Federico , Zamora Maria E , Urrutia Juan I , Laporta Jimena , Batista Dubeux Jose Carlos , Gonella-Diaza Angela M , DiLorenzo Nicolas 
Published: November 2020

Abstract
A completely randomized design study with a 2 × 2 factorial arrangement of treatments evaluated the impact of artificial shade (SHADE or NO SHADE) and breed (ANGUS vs. BRANGUS) on performance of cows, nursing calves, and subsequent offspring. Twenty-four Angus and 24 Brangus black-hided pregnant cows (579 ± 8 kg BW; 6.5 yr; approximately 85 d of gestation) and their nursing calves were randomly allocated to 12 ‘Pensacola’ bahiagrass pastures (Paspalum notatum Flüggé; 1.3 ha; n = 4 pairs/pasture), with or without access to artificial shade (NO SHADE BRANGUS [NSB], NO SHADE ANGUS [NSA], SHADE BRANGUS [SB], and SHADE ANGUS [SA]) for 56 d during summer. Body condition score (BCS) of cows and BW of pairs were obtained on d -1, 0, 55, and 56 (weaning weight). Following weaning, calves were randomly allocated to 4 pens (n = 12/pen) equipped with GrowSafe feed bunks for 14 d to measure feed intake (DMI) and efficiency (G:F). A shade × breed interaction (P &lt; 0.05) was observed for average daily gain (ADG) and BCS of cows, with SB being greatest (P ≤ 0.05). Pre-weaning calf ADG tended to be greater (P = 0.10) for SHADE vs. NO SHADE. Weaning weight and BW 14-d post-weaning were lesser for NSB vs. NSA, SA, and SB, whereas no differences in DMI, ADG, or G:F were observed (P &gt; 0.11). Gestation length was greater for SHADE vs. NO SHADE cows (292 vs. 274; P = 0.02), but calf birth weight was not different. Providing artificial shade to pregnant-lactating beef cows positively impacted the growth of Brangus but not Angus cows. However, weaning BW of calves from Angus cows regardless of shade access did not differ from that of Brangus calves with shade. Further research should investigate the potential long-term effects of shade on the subsequent offspring.
377 Validation and improvement of a monitoring system overtime and its overall capabilities
Authors: Jennings Tracy D , Nickell Jason 
Published: November 2020

Abstract
Automated animal monitoring is a growing segment in animal agriculture. These technologies capture biometric data from numerous parameters such as facial recognition, rumination, body temperature, and animal movement. The goal of automated animal monitoring technologies is to provide a 24 h perspective on an individual animal. These devices can offer valuable information pertaining to animal health, location, locomotion, animal behavior, phenotypic traits, and reproductive cycles. Devices collect data in various forms, such as counting steps, G.P.S. coordinates, triangulation, or XYZ coordinates. Raw data (e.g. step counts, XYZ coordinates) are then analyzed with proprietary algorithms to provide information in a usable format to the consumer. However, the development of these algorithms require thousands of hours of observation. For example, thorough visual observation of individual animal behaviors (e.g. rumination and eating time) can be identified based upon accelerometer movement. However, these calculated behaviors must then be validated through observation to optimize accuracy. Accelerometer-based devices are common in the dairy industry and growing in popularity in the beef industry for monitoring health and detecting estrus. Twenty-four-hour observation provides livestock producers greater insight than does once or twice daily observations. However, the usefulness or reliability of a technology requires evaluation of outcomes based on sensitivity and specificity of the test. The choice to optimize sensitivity or specificity is dependent on the desired outcome of the end user. For example, by increasing sensitivity in an animal health monitoring system, more truly ill animals will be identified, but more false positives will be generated, resulting in more animal treatments and elevated treatments costs. Conversely, increasing specificity, sick animals will be missed, but treatment costs will be reduced. Ultimately, developing an automated animal monitoring system that meets consumer expectation is the goal; however, information interpretation and evaluation will still be necessary by the end user.
202 Dietary supplementation with a blend of essential oils and a proprietary cobalt source along monensin may be antagonistic to growth performance of Holstein yearling bulls
Authors: Wu Jianping , Bai Yan , Lang Xia , Wang Cailian , Shi Xiaolei , Zhang Liping , Liu Haibo , Liu Ting , Gong Xuyin , Liang Tingyu , Zhang Rui , Casper David P 
Published: November 2020

Abstract
Our previous work has indicated that feeding a blend of essential oils and a proprietary cobalt source (EOC) in combination with monensin (MON) may not be mutually beneficial to dairy calf growth performance. To evaluate this observation further, a 240 d long-term growth experiment was conducted using 12 young growing Holstein bulls using a 2 × 2 factorial treatment arrangement. Main factors were EOC (Rum-A-Fresh, Ralco, Inc., Marshall, MN) and monensin (MON; Norvikon Biotechnology Co., Hunan, China) arranged in 4 treatments: 1) Control: ration fed without EOC or MON; 2) EOC: EOC fed at 26 mg/kg DM; 3) MON: MON fed at 25 mg/kg DM; and 4) EOC+MON: EOC and MON fed in combination. Holstein bulls were 70 d of age and similar in BW (93.3 ± 4.54 kg) and individually fed for 280 d. Body weights, frame measurements, and blood samples were collected monthly. Interactions of EOC by MON were detected for BW, BW gain, ADG, and a trend for feed conversion. Bulls fed EOC or MON demonstrated greater final BW (367.7, 385.2, 381.2, and 357.5 kg for Control, EOC, MON, and EOC+MON, respectively), while BW gains (277.2, 291.5, 284.8, and 265.2 kg) and ADG (1.16, 1.22, 1.19, 1.11 kg/d) were greatest for bulls fed EOC or MON compared with bulls fed EOC+MON, with bulls fed Control being intermediate and similar to bulls fed MON. Intake of DM was greater for bulls fed EOC (6.55, 6.99, 6.60, and 6.42 kg/d) compared with bulls fed remaining treatments. Feeding EOC or MON separately can improve growth performance of growing Holstein bulls. Why the combination of EOC and MON is antagonistic to growth performance of Holstein bulls is unknown.
192 Abundance of rumen methanogens did not differ in cattle that differed in residual feed intake
Authors: Freetly Harvey C , Lindholm-Perry Amanda K , Foote Andrew P , Bennett Sarah , Wells Jim E 
Published: November 2020

Abstract
Methane represents an energetic loss, and it has been proposed that its reduction may be associated with improved feed efficiency. Rumen methanogens produce most of the methane. Forty-nine crossbred heifers were individually fed a ration that consisted of 67.75% rolled corn, 20% wet distillers grain with solubles, 8% chopped alfalfa and 4.25% mineral/vitamin/rumensin mix for 84 d. Residual feed intake was the residual of the observed – predicted FI for the multivariate regression of FI on ADG and mid-metabolic BW for the population. Six heifers with the greatest RFI (1.75 ± 0.31 kg DM/d) and 6 with least RFI (-1.48 ± 0.31 kg DM/d) were selected. Rumen fluid was sampled from a tube passed through the mouth into the rumen. Rumen fluid was flash frozen in liquid N2 and stored at -80°C. After the first collection, heifers with greater RFI were pair fed to the less RFI heifers and rumen fluid was sampled 5 wk later. DNA was isolated from the rumen fluid and primer sets targeting total methanogens, Methanomicrobiales, Methanobacteriales, Methanosarcina, Methanobacterium, Methanobrevibacter ruminantium + Mbb. Cuticularis, and Methanobrevibacter smithii + Mbb. wolinii + Mbb. thaueri + Mbb. gottschalkii + Mbb. Woesei were amplified with real-time quantitative PCR (qPCR). Standard curves were generated to quantify copy number. There were no differences in copy number for the interaction of RFI classifications and sample period for all primer sets (P &gt; 0.18). There were no differences in (P &gt; 0.15) in RFI classification for all qPCR primer sets. There were fewer copies for the Methanobrevibacter ruminantium + Mbb. Cuticularis qPCR for samples collected in the first week compared to the 5 wk sample (P = 0.03); however, there were no difference for collection week for the other qPCR assays (P &gt; 0.16). USDA is an equal opportunity provider and employer.