bluuebunny/crossref_metadata_2025_split huggingface dataset:
Foveal Regeneration after Treatment of Acute Foveal Toxoplasmic Chorioretinitis
Authors: Abrishami Mojtaba , Hosseini Seyedeh Maryam , Momtahen Solmaz , Zamani Ghodsieh 
Published: January 2021

Abstract
Purpose: To report a patient with impaired vision due to foveal involvement of toxoplasmic chorioretinitis, who was successfully treated with intravitreal and oral therapy and led to successful visual and anatomic recovery.Case presentation: A thirty two-year-old man presented with three-day history of gradually decreasing visual acuity, redness, pain and photophobia of the right eye. Anterior chamber cellular reaction, vitritis and a white retinochoroiditis patch with adjacent retinal vasculitis in the fovea was suggestive of the toxoplasmic chorioretinitis. He was treated with intravitreal Clindamycin and Dexamethason injection followed by six-week regimen of Azithromycine, Trimethoprim-Sulfamethoxazole, and Prednisolone. In serial optical coherence tomography imaging, retinitis patch changed to cavitary foveal destruction. Fovea was regenerated gradually, and visual acuity was concurrently improved from counting finger 3m to 20/25. Conclusion: In foveal toxoplasmic chorioretinitis lesions, timely treatment is associated with retinal regeneration and visual improvement.
Prevalence of Helicobacter Pylori Genotypes and Their Association With Upper Gastrointestinal Diseases: a Cross Sectional Study in Southern Iran
Authors: Raufi Rahim , Shahriarirad Reza , Pishva SeyedehMaryam , Taghipour Nikta 
Published: January 2021

Abstract
Background: Investigating the prevalence of vacuolating cytotoxin (vacA), cytotoxin associated gene A (cagA), glm M genotypes, and subtypes of vacA of Helicobacter pylori (H. pylori) isolate in Jahrom, Southern Iran. DNA extracted from H. pylori samples retrieved from gastric biopsy isolated from 113 dyspeptic patients with positive rapid urease test (RUT). Genotyping was done by polymerase chain reaction (PCR) technique, using primers for vacA (s1a, s1b, s1c, s1, s2, m2, and m1), cagA, and glmM. Endoscopy was done for all the patients to screen upper gastrointestinal (GI) disorders. Results: GlmM was detected in 100% of the cases. VacA subtypes s1am2, s2m2, s1a, s1b, and s1c were detected in 27.9%, 25.6%, 50%, 3.5% and 2.4% of the isolates, respectively, while cagA was detected in 60.5% of the isolates. VacA alleles m1, s1, and s2 were detected in 54%, 50%, and 44% of isolates respectively. Also, 60.5% of the isolates were cagA-vacA-positive. A significant correlation was observed between vacAs1bm1 and gastroesophageal reflux disease (GERD) and glmM and normal esophagus. The presence of vacAs1bm1 and vacAs1bm2 has a significant association with gastric erythema. The presence of cagA showed a significant association with normal esophagus and hiatal hernia.Conclusions: In our research, the number of glmM and cagA positive isolates is higher among other genotypes and cagA is correlated with hiatal hernia, and normal esophageal finding is correlated with glmM genotype. There was no association between age or sex of the patients and bacterial genotype.
MYC amplification at diagnosis drives therapy-induced hypermutation of recurrent glioma
Authors: Wang Jiguang , Mu Quanhua , Chai Ruichao , Liu Hanjie , Yang Yingxi , Zhao Zheng , Lui Ming Hong , Bao Zhaoshi , Song Dong , Jiang Biaobin , Sa Jason , Cho Hee Jin , Chang Yuzhou , Chan Kaitlin Hao Yi , Loi Danson Shek Chun , Tam Sindy Sing Ting , Chan Aden Ka Yin , Wu Angela , Poon Wai San , Ng H.K. , Chan Danny , Iavarone Antonio , Nam Do-Hyun , Jiang Tao 
Published: January 2021

Abstract
Clonal evolution drives cancer progression and therapeutic resistance1-2. Recent longitudinal analyses revealed divergent clonal dynamics in adult diffuse gliomas3–11. However, the early genomic and epigenomic factors that steer post-treatment molecular trajectories remain unknown. To track evolutionary predictors, we analyzed sequencing and clinical data of matched initial-recurrent tumor pairs from 511 adult diffuse glioma patients. Using machine learning we developed methods capable of predicting grade progression and hypermutation from tumor characteristics at diagnosis. Strikingly, MYC copy number gain in initial tumors emerged as a key factor predicting development of hypermutation under temozolomide (TMZ) treatment. The driving role of MYC in TMZ-associated hypermutagenesis has been experimentally validated in a model of TMZ-induced hypermutator using both patient-derived gliomaspheres and established glioma cell lines. Subsequent studies showed that c-Myc binding to open chromatin and transcriptionally active regions increases the vulnerability of genomic regions to TMZ-induced mutagenesis. Consequently, MYC target genes, including the key mismatch repair genes, develop loss-of-function mutations, thus triggering the hypermutation process. This study reveals MYC as an early predictor of cancer evolution and provides a machine learning platform for predicting cancer dynamics to improve patient management.
Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Cirrhosis Model
Authors: Kawakatsu-Hatada Yumi , Murata Soichiro , Mori Akihiro , Kimura Kodai , Taniguchi Hideki 
Published: January 2021

Abstract

Liver transplantation is the most effective treatment for end-stage cirrhosis. However, due to serious donor shortages, new treatments to replace liver transplantation are sorely needed. Recent studies have focused on novel therapeutic methods using hepatocytes and induced pluripotent stem cells, we try hard to develop methods for transplanting these cells to the liver surface. In the present study, we evaluated several methods for their efficiency in the detachment of serous membrane covering the liver surface for transplantation to the liver surface. The liver surface of dipeptidyl peptidase IV (DPPIV)-deficient rats in a cirrhosis model was detached by various methods, followed by fetal livers from DPPIV-positive rats were transplanted. We found that the engraftment rate and area as well as the liver function were improved in rats undergoing transplantation following serous membrane detachment with an ultrasonic homogenizer, which mimics Cavitron Ultrasonic Surgical Aspirator® (CUSA), compared with no detachment. Furthermore, the bleeding amount was lower with the ultrasonic homogenizer method than with the needle and electric scalpel methods. These findings provide evidence that transplantation to the liver surface with serous membrane detachment using CUSA might contribute to the development of new treatments for cirrhosis using liver organoids.
hTERT Promotes CRC Proliferation and Migration by Recruiting YBX1 to Increase NRF2 Expression
Authors: Gong Chunli , Yang Huan , Wang Sumin , Liu Jiao , Li Zhibin , Hu Yuyang , Chen Yang , Huang Yu , Luo Qiang , Wu Yuyun , Xiao Yufeng 
Published: January 2021

Abstract
Background: High human telomerase reverse transcriptase (hTERT) expression is related to severe Colorectal Cancer (CRC) progression and negatively related to CRC patient survival. Previous studies have revealed that hTERT can reduce cancer cellular Reactive Oxygen Species (ROS) levels and accelerate cancer progression; however, the mechanism remains poorly understood. NFE2‑related factor 2 (NRF2) is a molecule that plays a significant role in regulating cellular ROS homeostasis, but whether there is a correlation between hTERT and NRF2 remains unclear. Herein, we sought to determine the relationship of hTERT and NRF2 in the progression of CRC and to elucidate the underlying molecular mechanisms.Methods: qPCR, Western blot, Immunohistochemistry, immunofluorescence assays were used to detect the mRNA and protein expression of hTERT, NRF2 and YBX1 in CRC cell lines and tissues.CCK8 and colony formation were used to detect the proliferation of CRC cells, transwell assay was used to detect the migration of CRC cells. Dual-luciferase reporter assays were used to detect the promoter activity of NRF2. DNA pull-down/MS analysis was used to identify NRF2 promoter binding proteins. ChIP-qPCR was used to detect the YBX1binding sequences of NRF2 promoter.Results: Both hTERT and NRF2 are highly expressed in CRC tissues and associated with poor diagnosis. hTERT increases NRF2 expression at both the mRNA and protein levels. hTERT increases CRC proliferation and migration by inducing NRF2 upregulation. Moreover, hTERT primarily upregulates NRF2 by increasing NRF2 promoter activity rather than by regulating NRF2 mRNA or protein stability, and hTERT recruits YBX1 to upregulate NRF2 promoter activity thus increases NRF2 expression and enhances CRC proliferation and migration.Conclusions: hTERT facilitates CRC proliferation and migration by upregulating NRF2 expression through the recruitment of the transcription factor YBX1 to activate the NRF2 promoter.
Comparison of Sacroiliac Biopsies and Magnetic Resonance Imaging Examinations in Non-Radiographic Axial Spondyloarthritis
Authors: Wang Dan min , Gong Yao , Hou Zhi duo , Liu Yuan , Peng Jian hua , Zhang Yu ping , Shen Feng cai , Liang Hong jin , Lin Ling , Xiao Zhengyu 
Published: January 2021

Abstract
Background : Early diagnosis and treatment are the key to improve the prognosis of axial spondyloarthritis (axSpA), therefore, we aimed to evaluate the diagnostic value of magnetic resonance imaging (MRI) and pathological examination of the sacroiliac joint (SIJ) for non-radiographic axSpA (nr-axSpA). Methods : Fine needle aspiration biopsy of bilateral SIJs was performed in 107 patients with nr-axSpA after MRI examination. The active inflammatory manifestations and chronic structural changes in SIJs were evaluated using MRI. The pathological changes of SIJ specimens were examined using microscopy. Results : Bone marrow edema (BME) was present in 67/214 joints on MRI (31.3%). The proportion of pannus formation and inflammatory cell infiltration were up to 63.8% and 53.4% in patients without BME on MRI, which were not significantly lower than 75.0% and 58.3% in patients with BME on MRI. The inflammatory infiltrating cells were mainly CD3 + T lymphocytes and CD68 + macrophages. In the group with normal cartilage on MRI, the proportions of chondrocytes and cartilage matrix degeneration were 22.2% and 44.9%，respectively, which was not significantly lower than those in the group with abnormal cartilage on MRI (35.7% and 46.4%). However, a significantly higher proportion of pannus invasion was observed in the latter (26.6% vs 57.1%, P =0.001). In the group with a normal bone plate on MRI, the incidence of subchondral pannus formation and bone plate destruction was as high as 74.0% and 71.7%, with no significant difference compared with that in the group with bone erosion on MRI (88.9% and 88.3%). Conclusions: Pathological examination is more sensitive for detecting inflammatory and structural changes in early sacroiliitis than MRI examination. Key words : non-radiographic axial spondyloarthritis, sacroiliac joint, aspiration biopsy, magnetic resonance imaging.
Characteristics of Inflammatory Storm in BALF of Severe Pneumonia in Children: A Case Control Study
Authors: Deng Fang , Cao Hui ling , Liang Xiao hua , Li Qu bei , Yang Yang , Zhao Zhi hua , Tan Jun jie , Fu Guo , Shu Chang 
Published: January 2021

Abstract
Background: It is difficult to collect bronchoalveolar lavage fluid (BALF) from children. There are few studies on inflammatory storms in BALF of children with pneumonia. Therefore, we will retrospectively investigate characteristics of inflammatory storms in BALF of children with pneumonia in our hospital.Methods: This retrospective study included 161 children with pneumonia. Pediatric pneumonia was divided into two groups: mild and severe pneumonia, according to the clinic diagnosis. All clinic information and data came from our hospital’s database. Blood and BALF samples were collected and measured by Clinical laboratory and Clinical Molecular Medical Center. Flow cytometry was performed to detect the levels of cytokines, including IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-α and IFN-γ. We performed receiver operator characteristic curve (ROC) and spearman correlation to evaluate the role of BALF inflammatory cytokines and cells in pneumonia.Results: 1) The levels of IL-6, IL-10, IL-17A, and TNF-α, and ratios of IL-6 to IL-10 and TNF-α to IL-10 in BALF of severe pneumonia group were higher than those of mild pneumonia group. 2) The number of inflammatory cells and the percentage of neutrophils in BALF of severe pneumonia group increased, and the percentage of macrophages decreased, compared with the mild pneumonia group. 3) ROC analysis showed that the levels of IL-6, IL-10 and TNF-α, and ratios of IL-6 to IL-10 and TNF-α to IL-10, the number of inflammatory cells and neutrophils, as well as the percentage of neutrophils exceeded the cut-off value (60.37pg/mL, 2.54pg/mL, 13.07pg/mL, 29.30, 1.57, 3140.00*109/L, 768.00*109/L, 43.00%, respectively) could be used to distinguish children with severe pneumonia from children with mild pneumonia. While the percentage of macrophages in BALF decreased to below the cut-off value (36.00%) also had the discrimination ability. 4) The BALF levels of IL-6, IL-10, TNF-α and IFN-γ were correlated, positively with the inflammatory cell count and the percentage of neutrophils, and negatively with the percentage of macrophages.Conclusion: In severe pediatric pneumonia, BALF inflammatory cytokines and inflammatory cell infiltration are more intense, especially above the cut-off value. This study demonstrates that these inflammatory signals are potential biomarkers for predicting the severity of pediatric pneumonia.Trial registration: This study approved by the Medical Research Ethics Committee of Children’s Hospital of Chongqing Medical University, registered in http://www.chictr.org.cn/, No. ChiCTR2000034048(registration date, June 22, 2020).
Clinical Effect of a New Type of Transpedicular Reducer for Fracture Reduction via Pedicle and Bone Grafting Combined With Pedicle Screw Fixation for Thoracolumbar Burst Fractures
Authors: cai menghan , Xin Zhijun , Kong Weijun , Du Qian , Ji Wenjun , Wu Fujun , Li Jin , He Jialin , Liao Wenbo 
Published: January 2021

Abstract
BackgroundShort-segment transpedicular screw fixation is a common method for the treatment of thoracolumbar burst fractures (TBFs). When it is used, the anterior middle columns of the fractured vertebral body lack good support, resulting in fractured vertebral bodies decreased height and difficult to reduct.Therefore,the purpose of this study was to evaluate the clinical effect of a new type of transpedicular reducer that we designed for fractured vertebral body reduction and bone grafting in the treatment of TBFs.MethodsA total of 9 patients with TBFs who agreed to be treated with the new transpedicular reducer for fracture reduction via pedicle and bone grafting combined with pedicle screw fixation were retrospectively analysed. We measured the anterior and middlel heights of the injured vertebrae, the ratios of the anterior and middlel heights of the injured vertebral body to the respective heights of the adjacent uninjured vertebral bodies (AVBHr and MVBHr, respectively), and the Cobb angle of patients at different stages before and after surgery, and evaluated the distribution of bone grafts and bone healing 3 days and 12 months after the operation.ResultsThe anterior height of the injured vertebrae before the operation; after application of the transpedicular reducer; and 3 days, 3 months, 6 months and 12 months after the operation were 17.56±3.74 mm; 27.70±2.53 mm; and 29.08±2.52 mm, 28.36±1.93 mm, 28.12±1.42 mm, and 27.96±0.72 mm, respectively. The mid-heights of the injured vertebrae were 21.36±4.20 mm; 26.74±1.00 mm; and 27.70±2.01 mm, 27.05±2.45 mm, 26.94±1.84 mm, and 26.83 ±2.45 mm, respectively. The Cobb angles of the injured vertebrae were 3.80±1.44°; 1.26±1.00°; and 0.72±0.70°, 1.03±0.65°, 1.12±0.63°, and 1.34±0.56°, respectively. The allogeneic bones were distributed in the anterior and middle columns 3 days after the operation, and the bone had healed well 12 months after the operation.ConclusionThe new transpedicular reducer has a good reduction, allogeneic bone support, and clinical treatment effect for TBFs through pedicle fracture reduction and bone grafting combined with pedicle screw fixation.
Asymptomatic Malaria And Glycemic Control Among Type 2 Diabetes Mellitus Patients In A Rural Tertiary Health Facility In Ido- Ekiti, Southwestern Nigeria- A Cross Sectional Study.
Authors: Ibrahim Azeez Oyemomi , Agbesanwa Tosin Anthony , Agboola Segun Mathew , Shabi Olabode Muftau , Ajetunmobi Adewumi Oluwaserimi , Ismail Waheed Olalekan 
Published: January 2021

Abstract
Background: : Despite the high burden of Asymptomatic Malaria and Type 2 diabetes mellitus in sub-Saharan African, limited data exist regarding their co-occurrence and its associated clinical impacts of asymptomatic malaria parasitaemia on Type 2 diabetes mellitus in rural setting. The purpose of this study is to determine the prevalence of glycemic control and asymptomatic malaria parasitaemia; and to investigate the influence of socio-demographic characteristics and asymptomatic malaria parasitaemia on glycemic control among patients with Type 2 diabetes mellitus.Methods: Hospital-based cross-sectional study was conducted on 150 T2DM patients at Federal Teaching Hospital, Ido-Ekiti, SouthWestern Nigeria, between April and September 2019. Systematic random sampling technique was adopted to recruit the respondents. Socio-demographic data were obtained using a semi- structured interviewer administered questionnaire and clinical files. Venous blood samples were collected and processed for glycosylated haemoglobin sugar estimation and malaria parasite detection by microscopy. Data were analyzed using SPSS version 20.0. Multivariate logistic regression analysis was used to identify the influence of asymptomatic malaria parasitaemia and the socio-demographic profile on glycemic control.Results: The mean age, mean glycemic control and mean parasite density of the respondents were 64.8±11.1 years, 6.9±2.1% and 1123.4±433.8 respectively. The prevalence of poor glycemic control and asymptomatic malaria parasitaemia were 30.0% and 14.0% respectively. Old age, female gender, lack of formal education and asymptomatic malaria parasitaemia were associated with poor glycemic control.Conclusion: The study revealed that Type 2 diabetes mellitus patients in Ido-Ekiti, Nigeria, harbor asymptomatic malaria parasitaemia and that Asymptomatic malaria parasitaemia, old age, female gender and illiteracy were negative predictors of glycemic control. Therefore, type 2 diabetes mellitus patient with poor glycemic control should be screened for asymptomatic malaria parasitaemia. Also, respondents with these identified predictors should be targeted with focus care and qualitative health education to improve their glycemic control level.
Pemberitahuan Retraksi Artikel: Pengaruh Pemberian Terapi Tambahan Curcuma longa dan Boswellia serrata pada NSAID Terhadap Aktivitas Fungsional pada Pasien Osteoarthritis
Authors: Pharmacon Editor 
Published: January 2021

Pemberitahuan retraksi artikel: Pengaruh Pemberian Terapi Tambahan Curcuma longa dan Boswellia serrata pada NSAID Terhadap Aktivitas Fungsional pada Pasien Osteoarthritis The Effect of Additional Therapy of Curcuma longa and Boswellia serrata on NSAIDs on Functional Activity in Osteoarthritis PatientsRizaldy Taslim Pinzon1*, Jessica Herwanto21Departemen Neurologi, Fakultas Kedokteran, Universitas Kristen Duta Wacana, Yogyakarta, Indonesia2Mahasiswa Program Studi Farmasi Fakultas Farmasi Universitas Sanata Dharma, Yogyakarta, IndonesiaArtikel ini telah diretraksi dari Pharmacon: Jurnal Farmasi Indonesia 17(2), 121-132, Desember 2020, atas permintaan dari penulis dan persetujuan dari dewan editor.
Peningkatan Motivasi dan Hasil Belajar Matematika Tentang Pecahan Melalui Model Find The Fib Pada Siswa Sekolah Dasar
Authors: Adianto Samsul 
Published: January 2021

Penelitian ini merupakan penelitian tindakan kelas yang bertujuan untuk meningkatkan motivasi dan hasil belajar matematika tentang pecahan melalui model Find The Fib pada siswa kelas 5C di Sekolah Dasar SDN 004 Samarinda Ulu. Latar belakang penelitian ini adalah ditemukan permasalahan belajar pada kelas 5C khususnya pada motivasi dan hasil belajar matematika. Subyek penelitian ini adalah seluruh siswa kelas 5C. Penelitian dilaksanakan sebanyak tiga siklus, yang terdiri 3 kali pertemuan dalam setiap siklusnya. Teknik pengumpulan data berupa lembar observasi dan hasil belajar siswa. Data yang diperoleh disajikan dalam rata-rata dan persentase. Dari hasil analisis ketuntasan belajar siswa mengalami peningkatan, siklus 1 (8%), siklus 2 (70%), siklus 3 (100%). Berdasarkan hasil penelitian disimpulkan bahwa melalui model Find The Fib dapat meningkatkan motivasi dan hasil belajar matematika kelas 5C. Dengan variasi pengajaran yang unik dan berkelompok, pembelajaran menjadi aktif, suasana kelas menjadi lebih hidup, menciptakan pengalaman belajar baru bagi siswa, sehingga menimbulkan gairah belajar serta motivasi belajar dalam diri siswa untuk menyukai matematika.
TAFSIR QS. AN-NUR 24:32 TENTANG ANJURAN MENIKAH (Studi Analisis Hermeneutika Ma’na Cum Maghza)
Authors: Herlena Winceh , Hasri Muads 
Published: January 2021

AbstractMarriage requires sufficient mental, social, and material readiness to build a household. However, the Al-Qur'an calls for marriage even in a state of poverty.This contradicts the present context which requires preparedness before marriage. This paper aims to explore further the recommendations for marriage in QS. An-Nur: 32 with a few questions. First, what did QS. An-Nur: 32 mean to order marriage even though he was in poverty?. Second, what is the significance of QS. An-Nur: 32 is contextualized in the present context?. This research will use the theory of hermeneutics ma'na cum maghza which was popularized by Sahiron Syamsuddin. This research concludes that the recommendation to marry in a destitute state is not the main purpose of QS.An-Nur: 32, but rather as a liberator for slaves, a recommendation to respect those who cannot afford it, as well as a recommendation to marry for those who are able. AbstrakPernikahan membutuhkan kesiapan mental, sosial, dan materi yang cukup untuk membangun rumah tangga. Namun al-Qur’an berkata lain, al-Qur’an menyerukan untuk menikah meskipun dalam keadaan fakir. Hal ini tentu saja mengalami kontradiksi dengan konteks sekarang yang mengharuskan kesiapan sebelum pernikahan. Oleh karena itu, tulisan ini bertujuan untuk menggali lebih lanjut anjuran menikah dalam QS. An-Nur: 32 dengan beberapa pertanyaan. Apa maksud dan tujuan QS. An-Nur: 32 memerintahkan menikah meskipun dalam keadaan fakir? kemudian bagaimana signifikansi dari QS. An-Nur: 32 dikontekstualisasikan dalam konteks sekarang? Untuk menjawab rumusan masalah di atas, penelitian ini akan menggunakan teori hermeneutika ma’na cum maghza yang dipopulerkan oleh Sahiron Syamsuddin. Dari penelitian yang dilakukan, maka dapat disimpulkan bahwa anjuran menikah dalam keadaan fakir bukanlah maksud dan tujuan utama dari QS. An-Nur: 32, melainkan sebagai pembebas bagi para budak dan hamba sahaya, anjuran untuk lebih menghargai orang-orang yang tidak mampu, serta anjuran menikah bagi yang telah mampu. Kata Kunci: Q.S. An-Nur, Menikah, Maghza.