bluuebunny/crossref_metadata_2025_split huggingface dataset:
African swine fever virus-like integrated elements in a soft tick genome – an ancient virus vector arms race?
Authors: Forth Jan H. , Forth Leonie F. , Lycett Samantha , Bell-Sakyi Lesley , Keil Günther M. , Blome Sandra , Calvignac-Spencer Sébastien , Wissgott Antje , Krause Johannes , Höper Dirk , Kampen Helge , Beer Martin 
Published: March 2020

AbstractBackgroundAfrican swine fever virus (ASFV) is the only known DNA-arbovirus and a most devastating suid pathogen that, originating from a sylvatic cycle in Africa, has spread to eastern Europe and recently reached western Europe and Asia, leading to a socio-economic crisis of global proportion. However, since neither closely related viruses nor integrated viral elements have yet been identified, ASFV evolution remains a mystery.ResultsHere, we show that soft ticks of theOrnithodoros moubatagroup, the natural arthropod vector of ASFV, harbour African swine fever virus-like integrated (ASFLI)-elements corresponding to up to 10% (over 20 kb) of the ASFV genome. Through orthologous dating and molecular clock analyses, we provide data suggesting that integration occurred over 1.47 million years ago. Furthermore, our data indicate that these elements, showing high sequence identities to modern ASFV, are maintained in the tick genome to protect the tick from infection with specific ASFV-strains through RNA interference.ConclusionWe suggest that this mechanism of protection, shaped through many years of co-evolution, is part of an evolutionary virus-vector “arms race”, a finding that has not only high impact on our understanding of the co-evolution of viruses with their hosts but also provides a glimpse into the evolution of ASFV.
Anti-microbial activity of potential probiotic lactic acid bacteria against Methicillin-Resistant Staphylococcus Aureus (MRSA)
Authors: Essayas Alazar , Pandit Sujata , Taneja Pankaj 
Published: March 2020

AbstractLactic acid bacteria (LAB)are the essential ingredients in probiotic foods, intestinal microflora, and dairy products able to cope up and exist in diverse environmental ranges. Samples were collected using sterile test tubes and transported to a laboratory in the icebox for further biochemical characterization. Gram test and catalase activity were examined after microscopically distinct colonies were sub-cultured to pure colonies based on standard gram and catalase test protocols. Subsequently, these bacteria were characterized for their ability to grow at various salt concentrations (5%,10%, and15%) and temperature gradients (15°C, 30°C, 45°C). Acid-tolerance was analyzed by growing the colonies in MRS broth adjusted to acidic pH (pH 3) and pH 7.2 (control). Bile tolerance of LAB isolates was assayed by growing in 0.3% bile-supplemented MRS agar. Bile salt hydrolase (BSH) activity was studied by growing 10 μl of the prepared overnight culture on BSH screening media containing MRS agar plate supplemented with bile salts. The LAB isolates were checked for antimicrobial activity by agar well diffusion assay. All isolates found gram-positive, catalase-negative and non-motile, convex elevation and entire margin. All LAB isolates were able to grow at 5-10% Nacl concentration, whereas moderately grow at 10% concentration but rarely grow at 15% Nacl concentration. BCM2, BBM3 and BGM1 record the highest acidic resistance viability percentage 94.9%, 92.7%, and 91.8% respectively. BCM3 has the lowest acidic resistance with a viable percentage of 87.4%. BBM1 records the highest bile tolerance activity whereas BCM2 has the lowest bile tolerance. All isolates were found BSH positive. The study reveals LAB isolates showed a putative probiotic potential.Article highlightsLAB are the main ingredients of probiotic products commercially available in the market nowadays. To effectively functioning the host gastrointestinal tract probiotics, need to have certain criteria like acid and bile tolerance this study revealsAcid tolerance activity of LAB isolated from bovine milkBile tolerance activity of LAB isolated from bovine milkBile salt hydrolase (BSH) activity of LAB isolatesAntimicrobial activity of LAB against MRSA
Evolutionary Distance of Gene-Gene Interactions: Estimation under Statistical Uncertainty
Authors: Gu Xun 
Published: March 2020

AbstractConsider the functional interaction of geneAto an interaction subjectX; for instance, it is the gene-gene interaction if X represents for a gene, or gene-tissue interaction (expression status) if X for a tissue. In the simplest case, the status of thisA-Xinteraction isr=1 if they are interacted, orr=0 otherwise. A fundamental problem in molecular evolution is, given two homologous (orthologous or paralogous) genesAandB, to what extent their functional overlapping could be by the means of interaction networks. Given a set of interaction subjects (X1, …XN), it is straightforward to calculate the interaction distance (IAB) between genesAandB, by a Markov-chain model. However, since the high throughput interaction data always involve a high level of noises, reliable inference ofr=1 orr=0 for each gene remains a big challenge. Consequently, the estimated interaction distance (IAB) is highly sensitive to the cutoff of interaction inference which is subject to some arbitrary. In this paper we will address this issue by developing a statistical method for estimatingIABbased on thep-values (significant levels). Computer simulations are carried out to evaluate the performance of differentp-value transformations against the uncertainty of interaction networks.
Public goods production and value creation in wineries: a structural equation modelling
Authors: Marotta Giuseppe , Nazzaro Concetta 
Published: March 2020

PurposeThe aim of the study is to analyse the value creation processes in multifunctional wineries. Specifically, the paper poses the following research questions: can the creation of public goods (i.e. multifunctionality) open up new paths of value creation for wineries? And if so, can multifunctionality be only compensated through policy instruments? Or, is there a form of market compensation?Design/methodology/approachAn empirical analysis was carried out on selected wineries that sell directly. The study implemented the “value portfolio” model that identifies specific variables, both internal and external to the farm, which contribute to the value creation. The methodology adopted is a structural equation modeling (SEM) approach that offers a theoretical basis for developing an understanding of the relationships between group of variables.FindingsThe empirical analysis confirms the assumptions developed. Indeed, the localized public goods generated by multifunctional farms can benefit from compensation on the market through the direct sale of products and services.Originality/valueThe study’s findings are quite innovative in the field of agricultural economics and open the way for interesting policy implications.
Output additionality of R&D subsidy on manufacturing venture firms in Korea
Authors: Kim KonShik 
Published: March 2020

PurposeThe purpose of this study is to determine the extent to which R&D subsidy can affect the innovation process of manufacturing venture firms by examining the output additionality measured as both proximal indicators of innovation and distal indicators of growth. Further, the differences in output additionality between the clusters in the subcontracting regime were examined to investigate whether the effect of R&D subsidy can vary depending on subcontracting practices and structure among large enterprises and venture firms.Design/methodology/approachThis study uses survey data of the Korea Venture Business Association conducted in 2012, 2013, 2014, 2015, and 2016 respectively, which selects a random sample from venture firms by stratified random sampling method based on the industry sector, size and location for each survey year. This study analyzed the data using an endogenous treatment effects model to estimate the average treatment effect of R&D subsidy, yielding more accurate estimates than a traditional treatment effects model by controlling the unobserved endogenous components.FindingsThis research found that R&D subsidy may not facilitate the process of transformation of innovation into financial growth even though R&D subsidy can facilitate the innovation process and contribute to producing new and improved products. This research also reveals that the relationship between R&D subsidy and innovation performance for firms heavily dependent on subcontracting is generally much weaker than those for independent subcontractors. Further, the present study exhibits that public R&D subsidy for independently subcontracting venture firms is more effective for the growth in both employment and sales than those for subcontracting with large enterprises or other subcontractors.Research limitations/implicationsR&D subsidy for venture firms does not relieve the burden of liability of newness and smallness of venture firms, especially the disadvantage in market penetration and competition. In addition, venture firms subcontracting with large enterprises or other prime subcontractors tend to achieve incremental innovation with the help of the technology and competence of large companies and run stable businesses through a predetermined market.Practical implicationsR&D subsidy for venture firms does not relieve the burden of liability of newness and smallness of venture firms, especially the disadvantage in market penetration and competition. Further policy measures should be implemented so as to identify and eliminate barriers to market acceptance for new products of venture firms.Originality/valueThis research verifies that the effect of R&D subsidy may harmful to the sales growth of venture firms and the output additionality differs with the degree of dependency on subcontracting practices and structure.
The relationship between leadership styles and organisational innovation
Authors: Alblooshi Mohamed , Shamsuzzaman Mohammad , Haridy Salah 
Published: March 2020

PurposeThis study explores the role of leadership in organisational innovation by reviewing several publications that discuss the relationship between various leadership styles and innovation.Design/methodology/approachThe study followed a descriptive research methodology by reviewing 64 journal articles on the relationship between various leadership styles and innovation. The articles were analysed descriptively and then reviewed based on the leadership style it discusses to derive meaningful findings on the relationship between leadership and innovation.FindingsVarious leadership styles had a positive impact on organisational innovation either directly or indirectly, by influencing the organisational climate, employees' and leaders' behaviours or other organisational variables such as learning and knowledge sharing. Some leadership styles had both direct and indirect impacts on organisational innovation.Research limitations/implicationsThis study collected journal articles published in almost all major electronic databases such as Emerald, ScienceDirect, Taylor & Francis and Scopus. However, the review is limited to journal articles in which the title, abstract or author-specified keywords contain the search terms “leadership” and “innovation,” and published between 2000 and 2019. Therefore, this review may miss some relevant research insights mentioned in the literature that discussed innovation or leadership separately not combined.Originality/valueThis study contributes to the existing body of research on leadership and innovation by extensively discussing the role of various leadership styles in determining organisational innovation. The analysis reveals that prior studies had many limitations and focused on specific leadership styles only. The study goes a step further by explaining how the leadership and innovation aspects are related, and classifying various leadership styles according to their impact on organisational innovation being direct, indirect or both.
Line management involvement in performance appraisal work
Authors: Tyskbo Daniel 
Published: March 2020

PurposeTwo research questions are asked in this paper: RQ1. How does line management involvement in PA work unfold in practice? RQ2. How does line management involvement contribute toward any divergence arising between intended and implemented PA work?Design/methodology/approachAn in-depth case study from a multi-actor perspective based on interviews with HR managers, line managers and employees, and organizational documents.FindingsThe findings illustrate how line managers faced three types of complexities during implementation, i.e. dilemmas, understandings, and local adaptations. These jointly contributed to a divergence arising between the PA as intended and the PA as implemented. This divergence became associated with how line management involvement was restricted to the local context and the initial stages of the PA process, highlighting how HR practices can contain both devolved and non-devolved elements.Originality/valueWe respond to calls for more in-depth qualitative studies of how line managers are involved in HR work; this is done specifically by conceptualizing the complexities line managers face in practice when implementing HR practices. As such, we add to the understanding of HR practices as relational and social in nature. We also contribute to the processual understanding of HRM by highlighting how HR practices can contain both devolved and non-devolved elements. By stressing the limitations of binary conceptualizations of HR devolution, we add to the understanding of HR devolution as more complex and multifaceted than traditionally assumed.
The dynamic role of rapport on satisfaction–commitment relationship
Authors: Fatima Johra Kayeser , Mascio Rita Di , Quazi Ali , Johns Raechel 
Published: March 2020

PurposeThis study aims to capture the mediation role of customer–frontline employee rapport on customer satisfaction and affective, calculative and normative commitment by using three alternative models. It also verifies the moderation effect of relationship age on the rapport-satisfaction link in each alternative model.Design/methodology/approachThe survey data collected from bank customers were analyzed using structural equation modeling (SEM) with the partial least square (PLS) method.FindingsResults confirmed rapport as a significant mediator between satisfaction and each of the three types of commitment. Relationship age significantly moderates the links between rapport to affective and normative commitment but not to calculative commitment.Research limitations/implicationsAdditional findings from “importance–performance analysis” suggest that satisfaction is more import to customers than rapport for developing commitment, so further investigations can reveal the underlying reasons. Also, complementary mediation shows one or more missing mediators, which calls for future research.Practical implicationsManagers need to use rapport strategically with customers in different relationship ages to build different types of commitment. Specific tactics to build rapport and possible long run implications for developing affective, calculative and normative commitment have been discussed in the “note to practitioner” section.Originality/valueUsing “broaden-and-build” theory, the study extends the literature by confirming the mediation influence of rapport on satisfaction and three types of commitment relationships.
The impacts of relational capital on self-disclosure in virtual communities
Authors: Lee Yi-Hsuan , Hsiao Chan , Weng Jingjing , Chen Yi-Hsuan 
Published: March 2020

PurposeThis study examines whether relational capital influences self-disclosure behavior through the mechanism of needs-based motivation in virtual communities.Design/methodology/approachThis study adopts hierarchical linear model (HLM) to differentiate between the relationships at different levels, with 378 online questionnaires recovered from 42 virtual communities.FindingsThe results show that group-level relational capital is positively related to self-disclosure and affects it through the partially mediating mechanism of motivation. Relational capital also strengthens the positive influence of the need to be on trend on individual self-disclosure behavior.Originality/valueThis study makes four research contributions. Firstly, we identify the means by which relational capital established within a virtual community influences user disclosure behavior. This focus differs from those of previous studies, which have emphasized privacy and security of information systems, cost–benefit considerations, and/or adopted personality traits as the research basis. Secondly, this study examines and verifies the mediating mechanism of motivation, establishing an alternative perspective for theoretical studies, and providing future studies with a reference for investigating the self-disclosure behavior of members. Thirdly, this research introduces and verifies the moderating effects of relational capital based on member relationships, thus making further theoretical and empirical contributions. Finally, we adopt HLM to conduct our analyses, thereby ensuring higher precision regarding the explanatory power of group-level explanatory variables for individual-level dependent variables.
Ex-post effects of circuit breakers in crisis and calm markets
Authors: Sifat Imtiaz , Mohamad Azhar 
Published: March 2020

PurposeDespite regulatory claims of straitening volatility and preventing crashes, evidences on circuit breakers' ability to achieve so are nonconclusive. While previous scholars studies general performances of circuit breakers, the authors examine whether Malaysian price limits aggravate volatility, impede price discovery, and interfere with trading activities in both tranquil and stressful periods.Design/methodology/approachThe study uses a combination of parametric and nonparametric techniques consistent with Kim and Rhee (1997) to examine the major ex-post hypotheses in circuit breaker research.FindingsFor calm markets, the authors find significant success of upper limits in tempering volatility with low trading interference. Lower limits show mixed results. Conversely, in crisis markets limits fare poorly in nearly all aspects, particularly for lower limits.Practical implicationsRamifications of the paper's findings are discussed through highlighting the asymmetric nature of price limits' ex-post effects. The paper also contributes to regulatory debate surrounding the quest for an optimal price limit.Originality/valueThe paper is the first of its kind in documenting long-horizon evidence of ex-post effects of a wide-band price limit. Moreover, the paper is unique in its approach in bifurcating circuit breaker performance along the line of market stability periods.
The sixth estate: tech media corruption in the age of information
Authors: Spence Edward Howlett 
Published: March 2020

Purpose
The purpose of this paper is to demonstrate how some of the information and communication practices of the Tech Media and specifically of Facebook, constitute media corruption. The paper will examine what the professional role of Facebook is regarding its information/communication practices and then demonstrate that Facebook is essentially a media company and not merely a “platform,” therefore liable to the same normative responsibilities as other media companies.

Design/methodology/approach
Applying the dual obligation information theory (DOIT), a normative information and communication theory that applies generally to all media companies that disseminate and share information, the paper demonstrates that Facebook’s role of mediating and curating the information of its users places upon it a normative editing responsibility, to ensure both the preventive detection and corrective editing of fake news, as well as other forms of misinformation disseminated on its platform. Finally, applying a philosophical model of media corruption the paper will demonstrate that Facebook’s role in the Cambridge Analytica case was not only unethical but moreover, constituted media corruption.

Findings
The paper concludes that Facebook’s media corruption illustrated in the Cambridge Analytica case is not a one-off case but the result of a systemic and inherent conflict of interest between its business model of selling users’ information to advertisers and its normative media role rendering the conflict of interest between those two roles conducive to media corruption.

Originality/value
The paper's originality is twofold. It demonstrates that Facebook is a media company normatively accountable on the basis of an original theory the DOIT and moreover, on the basis of an original media corruption theory its actions in the Cambridge Analytica case constituted media corruption.
How does concrete strength affect the fire resistance?
Authors: Lubloy Eva 
Published: March 2020

Purpose
The aim of the research was to investigate the effect of concrete strength on the fire resistance of structures. At first, it may seem contradictory that higher concrete strengths can decrease the fire resistance of building structures. However, if the strength of the concrete exceeds a maximum value, the risk of spalling (the detachment of the concrete surface) significantly.

Design/methodology/approach
Prefabricated structural elements are often produced with higher strength. The higher concrete strengths generally do not cause a reduction in the load bearing capacity, but it can have serious consequences in case of structural fire design. Results of two prefabricated elements, namely, one slab (TT shaped panel) and one single layer wall panel, were examined. Results of the specimen with the originally designed composition and a specimen with modified concrete composition were examined, were polymer fibres were added to prevent spalling.

Findings
As a result of the experiments, more strict regulations in the standards the author is suggested including more strict regulations in the standards. It has been proved that to ensure the fire safety of the reinforced concrete structures, it is required after polymer fibres even in lower concrete strength class than prescribed by the standard. In addition, during the classification and evaluation of structures, it is advisable to introduce an upper limit of allowed concrete strength for fire safety reasons.

Originality/value
As a result of the experiments, the author suggests including more strict regulations in the standards. It has been proved that to ensure the fire safety of the reinforced concrete structures, it is necessary to require the addition of polymer fibres even in lower concrete strength class than prescribed by the standard. In addition, during the classification and evaluation of structures, it is advisable to introduce an upper limit of allowed concrete strength for fire safety reasons.