bluuebunny/crossref_metadata_2025_split huggingface dataset:
Menstrual hygiene management practice and factors affecting it among high school females in Ambo City, Oromia state, Ethiopia, 2018: A cross-sectional mixed method
Authors: Shallo Seifadin Ahmed , Willi Wakeshi , Abubekar Abuzumeran 
Published: February 2020

Abstract
Background Though menstruation is normal physiology in the females’ life, if not managed safely, it can interrupt daily activity or it may lead to health problems. Poor water, sanitation and hygiene (WASH) facilities in schools, inadequate puberty education and lack of hygienic menstrual hygiene management items (absorbents) cause girls to experience menstruation as shameful and uncomfortable. This study aimed to assess the extent of unsafe menstrual hygiene practice and factors influencing it among school females in Ambo, Ethiopia.Methods An institutional-based mixed-method cross-sectional study was conducted from March 01 to 15/2018 to collect data from 364 school females, teachers, and investigators observation. To collect the data, self-administered technique, interview, observational and FGD techniques were used. Data were analyzed using SPSS statistical software version 20. Uni-variate, bivariate and multiple logistic regression analysis were done. With 95% CI, the P-value of less than 0.05 was taken as the level of significance.Results The prevalence of the unsafe menstrual hygiene management practice was 53.6%, which implies urgent response from the stakeholders is of paramount importance. Factors such as the age of the females, frequency of discussing menses with mothers and source of information about menses were variables significantly associated with menstrual hygiene management practice.Conclusion and Recommendation High numbers of school females’ menstrual hygiene management were poorly managed. This implies urgent measure is needed from the stakeholders to solve these problems so that sustainable development goal number 3, 4 and 5 will be achieved. To rid off the current problems which school females are facing, comprehensive and different sectors collaboration is important. Specifically, education sectors, water and sanitation sectors, and health sectors bear the frontline responsibilities.
Whole-genome sequencing of a multiple endocrine neoplasia type 1 proband identifies gross deletion in MEN1
Authors: Naruoka Akane , Ohnami Sumiko , Nagashima Takeshi , Serizawa Masakuni , Hatakeyama Keiichi , Ohshima Keiichi , Ohnami Shumpei , Urakami Kenichi , Horiuchi Yasue , Kiyozumi Yoshimi , Matsubayashi Hiroyuki , Abe Masato , Ohishi Takuma , Kameya Toru , Sugino Takashi , Onitsuka Tetsuro , Isaka Mitsuhiro , Ohde Yasuhisa , Sugiura Teiichi , Ito Takaaki , Uesaka Katsuhiko , Akiyama Yasuto , Kusuhara Masatoshi , Yamaguchi Ken 
Published: February 2020

Abstract
BackgroundMultiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant tumor syndrome with neuroendocrine tumorigenesis of the parathyroid and pituitary glands and pancreatic islet cells. This hereditary cancer is caused by germline mutations in the MEN1, located on chromosome 11q13. Among the approximately 3,000 cancer patients, in which multi-omics analysis was performed, we had a patient with multiple tumors including pancreatic neuroendocrine tumor, thymic carcinoid and adenoma parathyroid, who showed no family history of MEN1.MethodsWe performed whole genome sequencing (WGS) of peripheral blood samples and three isolated tumor tissues (pancreas, thymus, and parathyroid). Sanger sequencing was used to validate MEN1 mutations; meanwhile, MEN1 mRNA levels on tumor samples were obtained by microarrays. MEN1 copy numbers and protein expression levels were determined by real-time PCR, and immunohistochemistry, respectively.ResultsMEN1 mRNA levels of pancreas and parathyroid tissues from patient-derived tumor samples were remarkably reduced, compared to those of 3,095 tumor and normal tissue samples. WGS data indicated the thymus sample with MEN1 nonsense mutation (W203*), while no MEN1 mutations were observed in the other two samples. WGS also identified MEN1 gross deletion of 18.5 kbp (chr11:64569322 – chr11:64587796; GRCh37/hg19 assembly) in peripheral blood samples and all the three tumor tissues. In addition, no MEN1 copy numbers on pancreas and parathyroid tumors were detected, whereas peripheral blood and the thymic carcinoid had one copy. Immunostaining of all the tissues detected very low levels of the MEN1 protein, Menin.ConclusionWe detected a hemizygous MEN1 germline deletion in blood sample of the MEN1 proband. All the three tumor samples had second hit with a deleterious mutation or normal alleles lacked to generate loss of heterozygosity at the MEN1 locus, suggesting loss of tumor suppressive function for MEN1.
Incidence and Mode of Deployment Failure of the Fast-Fix 360™ Meniscal Repair Device
Authors: Lawrie Steven Alan , Lawrie Angus , Vertullo Christopher 
Published: February 2020

Abstract
Background Meniscal repair device deployment failure is a recognised complication of all-inside meniscal repair with subsequent risk of patient harm. The Fast-Fix device has undergone design changes to reduce deployment failure since its introduction in 2001. Purpose The purpose of this study was to assess the incidence and mechanism of intra-operative deployment failure of the 3 rd generation Fast-fix 360™ (Smith & Nephew, Andover, USA) device. Method Data was prospectively collected for 106 consecutive all-inside meniscal repairs undertaken with the Fast-fix 360™ device. Patient demographics, mechanism of failure, site and type of repair were recorded. Results 20 deployment failures occurred in 423 Fast-fix 360™ deployments, an incidence of 4.72%. Deployment failure occurred in 19 of 109 patients an incidence of 17.43%. Six different failure mechanisms occurred; anchor exiting device without trigger being initiated, anchors not holding in meniscus, both anchors simultaneously exiting device and braided suture failing. The most common failure mechanism was failure of the second anchor not holding in the meniscus. Average cost of device failure was $75 per patient.Conclusion Despite design modification, deployment failure still occurs with similar modes top to the original design.
Staphylococcus Aureus Biofilm Evolution and Povidone-Iodine (PVP-I) Treatment Efficacy
Authors: chen mingli , Chen Zhi-da , Wu Qing-quan , Dai Li-lin , Wu Jin , Zeng Wen-rong 
Published: February 2020

Abstract
Objective The aim of this study is to observe the evolution changes of staphylococcus aureus biofilm on the surface of titanium alloy and evaluate bacterial changes of these biofilm after 0.5%g/L povidone–iodine treatment through the in vitro experiment. We have done research on this to provide some clinical guidance. Methods First, each period of 7days, 14days, 21days and 28days staphylococcus aureus biofilm models were established on the surfaces of titanium alloy and the biofilm morphology were detected by the confocal laser scanning microscope and scanning electron microscope. Then, morphological structure and the effect of 0.5%g/L povidone–iodine were further evaluated by viable count method after These biofilm were soak in the0.5%g/L povidone–iodine for5 min and 10 min respectively. Results 1. the extracellular polymer of biofilm was increasing with the Among them, the most obvious change and maturity occurred at the 21days and 28days respectively. With the process of biofilm maturation, the number of bacteria was decreased but the bacteria close to 90,000 and there was no significant difference (P>0.05). 2. The effect of 0.5%g/L povidone–iodine sterilization was significant,0.5%g/L povidone–iodine sterilization ten minutes group was better than PBS group(no intervention group) (P<0.05),0.5%g/L povidone–iodine sterilization five minutes was better than PBS group too (P<0.05). However, with the maturation of the biofilm, but not maturity 0.5%g/L povidone–iodine sterilization effect weakened but the 21days0.5%g/L povidone–iodine sterilization ten minutes was better than five minutes(P<0.05).there was no significant difference in maturity(P>0.05). Conclusion 1. The maturation of the biofilm is bacteria and outside then formation of extracellular polymeric substance and they formation micro environment together and the 21days is the time critical point for distinguishing young biofilm and mature biofilm, the most difference of which is the extracellular polymers and micro-environment.2. Early Implant related biofilm 0.5%g/L povidone–iodine treatment for 10 min is better than 5 min, but there is no extension meaning of static after maturity. So for early clinical implant related infections0.5%g/L povidone–iodine sterilization 10 min is accepted.
Enhanced β-carotene production by promoting the multivesicular body (MVB) pathway in Yarrowia lipolytica
Authors: Yang Fan , Liu Liang , Qiang Shan , Hu Ching Yuan , Li Ying , Meng Yong Hong 
Published: February 2020

Abstract
Background: β-carotene is a precursor of vitamin A and has great commercial value as an additive in foods and feeds. Many pathways not directly related to the β-carotene synthesis affect β-carotene production since the interactions among metabolic fluxes of cells confer a complex regulatory network. Engineered Y. lipolytica strain has excellent potential for β-carotene production as oleaginous yeast. Optimizing indirectly metabolic pathways in Y. lipolytica may offer a new strategy for making the β-carotene production achieve a commercially viable yield.Results: In this study, we found that the proper promotion of the multivesicular body (MVB) sorting pathway elevated the production of β-carotene by 1.58 fold when overexpressing one copy of the Did2 gene in Y. lipolytica. Through the measurement of ATP, NADPH, the mRNA, and protein level of key genes in the β-carotene synthesis pathway, the reason for β-carotene elevated was deuced that the protein level of the key enzymes (tHMG and CarA) was increased. When overexpressing two copies of the Did2 gene, the transcription level of the key genes was all elevated. However, the protein level of key enzymes in the β-carotene synthesis pathway was reduced compared with the overexpressing one copy of the Did2 gene, which resulted in reduced β-carotene content.Conclusion: This study suggests that the MVB sorting pathway is not responsible for sorting protein but has a crucial regulating effect on protein abundance in cells. Engineering the MVB sorting pathway could potentially increase the production of other high-value products. Moreover, manipulation of indirectly related metabolic pathways also is a critical strategy in synthetic biology research.
The effect of joint pain and arthritis on the Montana ranching and farming community
Authors: Webber Eliza J , Tran Tan , June Ronald , Healy Emily , Andrews Tara M , Younkin Roubie , MacDonald Justin , Adams Erik S 
Published: February 2020

Abstract
Background: Arthritis and joint pain have shown high prevalence rates in agricultural workers and is of concern because many agricultural operations are sustained by a small number of workers. The disability of even one worker may therefore contribute to economic hardship. This study investigated joint health in Montana agricultural workers and its economic effects on ranches and farms. Methods: The aims of this study were to determine associations between years working in agriculture and joint health, and between joint health and the economic health of a ranch or farm. A total of 299 ranchers and farmers in nine Montana counties completed a survey that included an assessment of the economic health of the ranch or farm, work capacity, and the need to rely on others to complete one’s work. Focus groups were then held with ranchers in two Montana counties, discussing similar topics. Results: 87.6% of survey respondents reported joint pain, 47.8% a diagnosis of arthritis, and 22.4% osteoarthritis (OA). Using proportional odds regression analysis, a 10-point increase in the Western Ontario and McMaster University arthritis index (WOMAC) score was significantly associated with lower work capacity (OR 2.00; 95% CI [1.58, 2.55], p<0.01), worse financial condition (OR 1.23; 95% CI [1.01,1.48], p=0.04), and increased reliance on others (OR 1.82; 95% CI [1.32, 2.55], p<0.01). A diagnosis of arthritis was associated with moving to a worse category of work capacity (OR 4.66; 95% CI [2.71, 8.01], p<0.01) and increased odds of relying on others (OR 3.23; 95% CI [1.56, 6.66], p<0.01), compared to those without arthritis. A diagnosis of OA was significantly associated with decreased work capacity (OR 3.47; 95% CI [1.97, 6.11], p<0.01). Adjusting for age and BMI, we found no association between years spent working in agriculture and joint health. Focus group themes included decreased productivity with increased joint symptoms and a tendency for ranchers to avoid interaction with the health care system. Conclusions: This mixed-methods study demonstrated an association between poor joint health and economic risk on Montana ranches and farms.
Participants’ satisfaction on China's New Rural Cooperative Medical System:a cross-sectional Survey in Liaoning province of China.
Authors: Liu Chunping , Wu Yifan , Wei Jingjing , Hu Xinzhu , Jiang Chunling , Sun Shu , Sun Yang 
Published: February 2020

Abstract
Background:China launched New Rural Cooperative Medical System (NRCMS) in 2003. However, satisfaction and voluntary participation in NRCMS of Liaoning province was unknown after ten years launching of NRCMS. Methods: In 2014, a total of 1500 individuals from 12 general hospitals in Liaoyang City, Dalian City, Panjin City and Tieling City of Liaoning Province were investigated using self-administered questionnaire by trained staff. Univariate and multivariate logistic regression analyses were applied to find out the satisfaction with NRCMS and its influencing factors among population from Liaoning province. Results: Of all respondents, 180 individuals (13.8%) were highly or relatively satisfied with the program. Moreover, 1092 individuals (84.0%) were fairly satisfied with the program, whereas 29 individuals (2.2%) were not. Multivariate analysis showed the price and range of drug, reimbursement ratio, simplicity of reimbursement procedures and medical technology level of hospitals were the main factors influencing the satisfaction.Conclusion: The overall satisfaction degree of Liaoning province's participating in NRCMS participant is at the middle level. In the process of seeking medical treatment, efforts are made to improve the reimbursement level, simplify the reimbursement procedures, increase the types of drugs in the reimbursement catalogue, and simultaneously take effective measures to control drug prices.
Development of a novel three-lncRNA immune-related signature as a prognostic indicator for hepatocellular carcinoma
Authors: Hu Bo , Yang Xiao-Bo , Sang Xinting 
Published: February 2020

Abstract
Background: Hepatocellular carcinoma (HCC) is one of the deadliest malignancies. Currently, there is still a lack of effective treatment. Our purpose was to develop an immune-related prognosis lncRNA signature with regard to HCC.Methods: A total of 14,142 lncRNAs and 331 immune genes were obtained from The Cancer Genome Atlas (TCGA) and the Molecular Signatures Database to construct the immune-related lncRNAs co-expression networks. Moreover, the tumor samples collected from TCGA were randomized as training set and testing set, among which, the testing set and the entire set were used for verification. Subsequently, gene set enrichment analysis (GSEA) and principal component analysis (PCA) were employed for functional annotation.Results: An immune-related signature consisting of AC015908.3, AC068987.4 and AL365203.2 was identified among HCC patients. Under different conditions, patients in low-risk group exhibited longer overall survival (OS) than those in high-risk group (P < 0.001). Moreover, the as-constructed signature was an independent factor, which showed marked association with patient OS (P < 0.001, hazard ratio (HR) = 1.407). These findings were further validated in testing set and the entire set. Additionally, GSEA results revealed the different immune states between low-risk and high-risk groups. On the other hand, lncRNA-related mRNAs were also extracted to depict the networks.Conclusion: Our findings indicate that the three-lncRNA immune-related signature shows prognostic value for HCC.
Progesterone regimen impact on live birth rate and neonatal outcomes in artificial frozen-thawed embryo transfer cycles: a retrospective cohort study
Authors: Liu Yuan , Wu Yu 
Published: February 2020

Abstract
Background: Previous studies have demonstrated that singletons from frozen embryo transfer (FET) are heavier and longer-gestational-days at delivery than those from fresh embryo transfer. The amounts and routes of progesterone used in FET vary tremendously among different ART centers. Does different serum progesterone level induced by different progesterone regimens determine live birth rate and neonatal outcomes in hormone replacement therapy frozen-thawed embryo transfer (HRT-FET) cycles? Design: A cohort study of 856 HRT-FET cycles from a Chinese public fertility center. Data from patients undergoing their first FET cycles from 2015-2018 were extracted from the database. All patients had their first FET with two day2 or day3 embryos transferred. Endometrial preparation was performed with sequential administration of estrogen followed by progesterone 60mg per day intramuscularly or Crinone 90mg per day vaginally. Live birth was the primary outcome. Secondary outcome included clinical pregnancy rate, singleton birthweight, large for gestational age (LGA) rate, SGA rate and preterm delivery rate. Student’s t test, Mann-Whitney U-test, Chi square analysis and multivariable logistic regression were used where appropriate. Differences were considered significant if p<0.05. Results: No significant difference of live birth rate was found between different progesterone regimens (Adjusted OR 1.128, 95%CI 0.842, 1.511, p=0.420). Neonatal outcomes like birthweight, preterm delivery rate, SGA and LGA rate were not different between two progesterone regimens. Serum P level >41.82 pmol/L at 14 day post-FET was associated with higher live birth rate than serum P level ≤41.82pmolL in HRT-FET cycles when progesterone was intramuscularly delivered (Adjusted OR 1.690, 95%CI 1.002, 2.849, p=0.049). Birthweight and gestational weeks were not different between these two different P level groups. Conclusions: Progesterone vaginally or intramuscularly didn’t impact live birth rate and neonatal outcomes in artificial FET cycles. Relatively higher serum progesterone level induced by intramuscular regimen did not increase newborn birthweight or prolong gestational weeks compared to vaginal regimen. Intramuscular progesterone supplementation during HRT-FET cycles was associated with improved live birth rate when progesterone concentration at day 14 post-FET was higher than 41.82pmol/L.
Exploring Heterogeneity in Moral Terminology in Palliative Care Consultations
Authors: Broek-Altenburg Eline van den , Gramling Robert , Gothard Kelly , Kroesen Maarten , Chorus Caspar 
Published: February 2020

Abstract
Background High quality serious illness communication requires good understanding of patients' values and beliefs for their treatment at end of life. Natural Language Processing (NLP) offers a reliable and scalable method for measuring and analyzing value- and belief-related features of conversations in the natural clinical setting. We use a validated NLP corpus and a series of statistical analyses to capture and explain conversation features that characterize the complex domain of moral values and beliefs. The objective of this study was to examine the frequency, distribution and clustering of morality lexicon expressed by patients during palliative care consultation using the Moral Foundations NLP Dictionary.Methods We used text data from 231 audio-recorded and transcribed inpatient PC consultations and data from baseline and follow-up patient questionnaires at two large academic medical centers in the United States. With these data, we identified different moral expressions in patients using text mining techniques. We used latent class analysis to explore if there were qualitatively different underlying patterns in the PC patient population. We used Poisson regressions to analyze if individual patient characteristics, EOL preferences, religion and spiritual beliefs were associated with use of moral terminology.Results We found two latent classes: a class in which patients did not use many expressions of morality in their PC consultations and one in which patients did. Age, race (white), education, spiritual needs, and whether a patient was affiliated with Christianity or another religion were all associated with membership of the first class. Gender, financial security and preference for longevity-focused over comfort focused treatment near EOL did not affect class membership.Conclusions This study is among the first to use text data from a real-world situation to extract information regarding individual foundations of morality. It is the first to test empirically if individual moral expressions are associated with individual characteristics, attitudes and emotions.
End of life practice and perceptions among physicians and residents in Intensive Care Units managed by anesthesiologists: a French national survey
Authors: Garcia Julie , Ichai Carole , Quintard Herve 
Published: February 2020

Abstract
Background In France, death after decision to withdrawal or withholding sustaining life therapies concerns about 10% of intensive care unit (ICU) patients. Clayes-Leonetti law published in 2016 intend to frame our medical exercise. The purpose of this study is to review the current practice concerning end of life (EOL) management in French ICU. Methods Two national surveys were conduct, the first one consisted in 30 multiple choices questions about epidemiology, EOL decisions making, care organization and self-opinion sent by mail to senior physicians members of “Société Française d’Anesthésie Réanimation (SFAR)”. Evaluation of practices on analgesia and sedation was conduct with two clinical cases. The second survey consisted in 24 multiples choices questions sent by mail to residents members of “SFAR jeunes”. Results Between October 2016 and October 2017, 695 physicians participated to the study. They majority work in a medico surgical unit in an University Center. Withholding and withdrawal life sustaining therapies decisions occur after collegial meeting (88%), a third doesn’t report decision in medical record, 46% always include medical third in decision making and only a third of physicians collect anticipate directive. Majority of them continue support care on these patients. In half of cases they haven’t sedation and analgesia protocol. Only 5% of physicians are completely satisfied of EOL care, where a better pain monitoring support is expected. Conclusion Heterogeneity for EOL management is important between physicians, we need to improve care and set up protocols to help physicians particularly concerning analgesia and sedation. Trial registration NA