bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reparative Effect of Mesenchymal Stromal Cells on Endothelial Cells after Hypoxic and Inflammatory Injury
Authors: Sierra-Parraga Jesus Maria , Merino Ana , Eijken Marco , Leuvenink Henri , Ploeg Rutger J. , Møller Bjarne K. , Jespersen Bente , Baan Carla C. , Hoogduijn Martin J. 
Published: June 2020

Abstract
Background
The renal endothelium is a prime target for ischemia reperfusion injury (IRI) during donation and transplantation procedures. Mesenchymal stromal cells (MSC) have been shown to ameliorate kidney function after IRI. However, whether this involves repair of the endothelium is not clear. Therefore, our objective is to study potential regenerative effects of MSC on injured endothelial cells and to identify the molecular mechanisms involved.
Methods
Human umbilical vein endothelial cells (HUVEC) were submitted to hypoxia and reoxygenation and TNF-a treatment. To determine whether physical interaction or soluble factors released by MSC were responsible for the potential regenerative effects of MSC on endothelial cells, dose-response experiments were performed in co-culture and transwell conditions and with secretome deficient MSC.
Results
MSC showed increased migration and adhesion to injured HUVEC, mediated by CD29 and CD44 on the MSC membrane. MSC decreased membrane injury marker expression, oxidative stress levels and monolayer permeability of injured HUVEC, which was observed only when allowing both physical and paracrine interaction between MSC and HUVEC. Furthermore, viable MSC in direct contact with injured HUVEC improved wound healing capacity by 45% and completely restored their angiogenic capacity. In addition, MSC exhibited an increased ability to migrate through an injured HUVEC monolayer compared to non-injured HUVEC in vitro.
Conclusions
These results show that MSC have regenerative effects on injured HUVEC via a mechanism which requires both physical and paracrine interaction. The identification of specific effector molecules involved in MSC-HUVEC interaction will allow targeted modification of MSC to apply and enhance the therapeutic effects of MSC in IRI.
Interdigital Perineural Fibroma And A Care Pathway.
Authors: Price Mark , Bridgen Andrew 
Published: June 2020

Abstract
BackgroundInterdigital perineural fibroma (IPF) is a complex condition affecting the plantar nerve of the foot and the care pathway for its management is based upon a consensus of clinical opinions. AimThe aim of the study was to investigate the treatment outcomes within a podiatric surgical practice and produce a validated care pathway over a three-year period.MethodsA retrospective mixed methods design was used to allow combination of quantitative and qualitative data. 89 patients between 1/4/2014 and 1/4/2017 had completed PASCOM-10 audit data. The treatment options were investigated and the MOXFQ domains and PSQ10 outcome scores were subject to analysis with SPSS.FindingsThe mean age of patients in the study was 56 (range 36-86, SD 10.85) and 89% were female, 11% male. 749 patients out of the total caseload had local anaesthetic/steroid injections over the three-year period, of which 49% of the known injections were for Morton’s Neuroma. No outcome data was recorded for injections.89 patients went forward for surgical excision of the IPF. The surgical approach for excision was either dorsal (60.7%) or plantar (39.3%). All plantar incisions (transverse and linear) were analysed as a group for comparison against the dorsal approach. Using an independent t-test, there was no significant difference for dorsal or plantar approach or location of IPF across the three MOxFQ domains and PSQ10.Using a Pearson product moment correlation coefficient there was a moderately strong correlation between the PSQ10 and the MOxFQ domains (n=89, Walking and Standing: r=0.48, p<0.01; Pain: r=0.42, p<0.01; Social Interaction: r=0.40, p<0.01). Where increases in MOxFQ outcome score (amount of improvement) produces higher PSQ10 scores (greater patient satisfaction).ConclusionsMore audit data is required to assess the effectiveness of steroid and / or alcohol injections. Recommendation for practice is based on literature review. The surgical outcomes would suggest that the excision of IPF is an appropriate and effective technique and the surgical approach or location of pathology will not affect the clinical outcomes. A new care pathway for the management of IPF (Morton’s Neuroma) will be employed in practice and
Straight versus modular nail: Analysis of mechanical properties two knee arthrodesis methods.
Authors: Białecki Jerzy , Para Marcin , Sobolewski Andrzej , Kogut Maciej , Bartosz Paweł 
Published: June 2020

Abstract
Background Complications after arthroplasty often result in irreversible disability. In some cases for the extremity to be salvaged, the permanent knee joint arthrodesis is the last-chance procedure. Modular implant design simplifies surgical technique but modularity may potentially compromise mechanical strength of an implant. Mechanical properties of the implant are particularly important in case of knee arthrodesis without bone-on-bone contact where forces during gait and weight bearing are transmitted directly through the nail. The aim of this article was to perform comparative analysis of the mechanical properties of modular nail CHARFIX2 FN, when compared to the femoral nail, used for knee arthrodesis; and to analyze the effectiveness of treatment with use of this nail based on the observations of clinical cases. Methods Comparative analysis of: the static 4-point bending test, dynamic 4-point bending test and static torsion test. All tests were performed in accordance with requirements of ASTM F 1264. A clinical analysis of 5 cases, in which CHARFIX2 FN nails were used, was also performed. Results Based on the results of mechanical tests, the strength characteristics of CHARFIX2 FN nail have been found superior and more advantageous than corresponding features of the standard femoral nail. For CHARFIX2 FN nail, the median for flexural stiffness was almost 4 times higher and for maximum torque value was almost 2 times higher when compared to the femoral nail. Observations of the clinical cases gave satisfactory results. Conclusions The obtained mechanical tests present significant differences between CHARFIX2 FN and the femoral nail in mechanical strength and, therefore, its improved stability and safety for patients during walking. It can be used for permanent knee immobilization with satisfactory clinical results. The functional outcomes and subjective measurements of pain in patients treated with CHARFIX2 FN group are satisfying.
Pgrn Alleviates Cerebral Amyloid-β Burden and Cognitive Impairments via Mediating Neuroinflammation
Authors: Xu Wei , Tan Chen-Chen , Cao Xi-Peng , Yu Jin-Tai , Tan Lan 
Published: June 2020

Abstract
Background: Both progranulin (PGRN) and neuroinflammatory activities increased over the course of Alzheimer’s disease (AD). In this study, we set out to determine if cerebrospinal fluid (CSF) PGRN could be a marker of neuroinflammation, and if so, how it contributed to AD pathogenesis and cognitive impairments. Methods: A total of 965 participants from the Alzheimer's Disease Neuroimaging Initiative (ADNI) were grouped within the framework of A-T-N biomarker profile and clinical stage. Causal mediation analyses with 10,000 bootstrapped iterations were conducted to explore the mediation effects of neuroinflammatory markers on the associations of PGRN with amyloid burden indicated by CSF β-amyloid (Aβ) levels. The longitudinal influences of PGRN on cognition were tested. Results: Increases of CSF PGRN and multiple neuroinflammatory markers (sTNFR1, sTNFR2, TGF-β1, VCAM1, and ICAM1) were associated with tau-related neurodegeneration, but not with Aβ pathology. PGRN was positively linked with these neuroinflammatory markers only in the presence of tau pathologies (TN+). In TN+ profile, PGRN was associated with higher CSF Aβ42 via mediating neuroinflammatory markers and could also predict slower cognitive decline. The abovementioned associations became non-significant in TN- profile. Conclusions: PGRN could protect against Aβ pathology and cognitive impairments via modulating neuroinflammation that occurs with neuronal injuries.
Antimalarial Activity of Curcuma Caesia Against 3D7 and K1 Strains of Plasmodium Falciparum
Authors: Chaturvedi Monika , Rani Reena , Sharma Dushyant , Yadav Jaya Parkash 
Published: June 2020

Abstract
Background: Malaria is a severe and sometimes mortal tropical disease that spreads through parasites. The purpose of the study was to evaluate in vitro and in-silicoantiplasmodial potential of Curcuma caesia extracts against Plasmodium falciparum.Methods: Lack of a vaccine and the widespread resistance to antimalarial drugs have resulted in emphasis on novel antimalarial drugs development. Ethyl acetate and methanol extracts of Curcuma caesia were prepared and analysed for their antiplasmodial activity against Chloroquine sensitive (3D7) and resistant (K1) strains of P. falciparumusingfluorescence-based SYBR Green assay. The cytotoxicity tests were carried out using the verocell lines by MTT assay.The phosphoethanolamine methyltransferase enzyme ((PfPMT) essential for growth of Plasmodium falciparum was used as protein target for in-silicostudy.Result: Curcuma caesia ethyl acetate extracts showedpotentantiplasmodial activitywith IC50 values of 3.37 µg/ml and 1.53 µg/ml against 3D7 and K1 strain respectively.Docking results show that β-selinenol an oxygenized sesquiterpene had the free binding energy of -6.76 Kcal/mol.Conclusion: Sesquiterpene present in the Curcuma caesia extract was responsible for antimalarial potential analyzed by molecular modeling. The present findings, however preliminary in nature. Further studies are required to proven the antimalarial efficacy C. caesia by isolating the active compounds and in vivo mechanism study that may contribute to more effective development of antimalarial drugs in the future.
How Useful are Malaria Risk Maps at the Country Level? Perceptions of Decision-Makers in Kenya, Malawi and the Democratic Republic of Congo
Authors: Ghilardi Ludovica , Okello George , Nyondo-Mipando Linda , Chirambo Chawanangwa Mahebere , Malongo Fathy , Hoyt Jenna , Lee Jieun , Sedekia Yovitha , Parkhurst Justin , Lines Jo , Snow Robert W , Lynch Caroline A. , Webster Jayne 
Published: June 2020

Abstract
Background Declining malaria prevalence and pressure on external funding have increased the need for efficiency in malaria control in sub-Saharan Africa (SSA). Modelled Plasmodium falciparum parasite rate (PfPR) maps are increasingly becoming available and provide information on the epidemiological situation of countries. However, how these maps are understood or used for national malaria planning is rarely explored. In this study the practices and perceptions of national decision-makers on the utility of malaria risk maps, showing prevalence of parasitaemia or incidence of illness, was investigated.Methods A document review of recent National Malaria Strategic Plans was combined with 64 in-depth interviews with stakeholders in Kenya, Malawi and the Democratic Republic of Congo (DRC). The document review focused on the type of epidemiological maps included and their use in prioritising and targeting interventions. Interviews (14 Kenya, 17 Malawi, 27 DRC, 6 global level) explored drivers of stakeholder perceptions of the utility, value and limitations of malaria risk maps.Results Three different types of maps were used to show malaria epidemiological strata: malaria prevalence using a PfPR modelled map (Kenya); malaria incidence using routine health system data (Malawi); and malaria prevalence using data from the most recent Demographic and Health Survey (DRC). In Kenya the map was used to target preventative interventions including long lasting insecticide treated nets (LLINs) and intermittent preventive treatment in pregnancy (IPTp), whilst in Malawi and DRC the maps were used to target in-door residual spraying (IRS) and LLINs distributions in schools. Maps were also used for operational planning, supply quantification, financial justification and advocacy. Findings from the interviews suggested that decision-makers lacked trust in the modelled PfPR maps when based on only a few empirical data points (Malawi and DRC).Conclusions Maps were generally used to identify areas with high prevalence in order to implement specific interventions. Despite the availability of national level modelled PfPR maps in all three countries, they were only used in one country. Perceived utility of malaria risk maps was associated with the epidemiological structure of the country and use was driven by perceived need, understanding (quality and relevance), ownership and trust in the data used to develop the maps.
Coronavirus disease 2019 may be driven by an overactivation of the renin-angiotensin-aldosterone system
Authors: Rysz Susanne , Al-Saadi Jonathan , Farm Maria , Jalde Francesca Campoccia , Klein Margareta , Vargas-Paris Roberto , Nyrén Sven , Abdula Goran , Ouellette Russell , Granberg Tobias , Fagerlund Malin Jonsson , Lundberg Johan 
Published: June 2020

Abstract
SARS-CoV-2 enters the cell through the ACE2 receptor, which is considered one of the main inhibitors in the Renin-Angiotensin-Aldosterone System (RAAS).1​ ,2 The virus has been shown to downregulate the ACE2 receptor, leading to a subsequent increase in the vasopressoragentangiotensinII.3​ Evidently,criticalcoronavirusdisease2019(COVID-19)is thought to be due to a dysregulated immune response, causing a cytokine-release syndrome eventually leading to acute respiratory distress syndrome (ARDS).4​ ,5 ​However, several reports on clinical laboratory features and case-descriptions of critically ill patients with COVID-19 show discrepancies compared to typical ARDS. Here, we show that infusing swines with angiotensin II induces a pathophysiological syndrome closely resembling that of patients with RT-PCR-positive COVID-19. By using multimodal clinical imaging of patients, comparing laboratory data and translational histological features, we show that it is highly likely that an increase in RAAS is one, if not the main, pathogenic feature in critical COVID-19. Furthermore, it is plausible that this large animal model can be used to screen for potential new treatments for patients with severe COVID-19 and that MRI lung perfusion can be used to evaluate the outcome of potential treatments targeting the pathophysiological syndrome.
Analysis of 4 imaging features in patients with COVID-19
Authors: JIN JUN , Gao De-hong , Mo Xin , Tan Si-ping , Kou Zhen-xia , Chen Yi-bo , Cao Jin-bo , Chen Wen-jing , Zhang Ya-ming , Li Bing-qing , Huang Kuan-long , Xu Bing-ren , Tang Xiao-li , Wang Yu-li 
Published: June 2020

Abstract
Purpose: This study aimed to analyze 4 chest CT imaging features of patients with coronavirus disease 2019 (COVID-19) in Shenzhen, China in order to improve the diagnosis of COVID-19 . Methods: Chest CT of 34 patients with COVID-19 confirmed by the nucleic acid test (NAT) were retrospectively analyzed. A nalyses were performed to investigate the pathological basis of 4 imaging features ( “feather sign” , “dandelion sign” , “pomegranate sign”, and “rime sign” ) and to summarize the follow-up results. Results: There were 22 patients (65.2 %) with typical “feather sign”, and 18 (52.9 %) with “dandelion sign”; few patients had “pomegranate sign”, and “rime sign”. The“feather sign” and “dandelion sign” were composed of stripe or round ground-glass opacity ( GGO ) , thickened blood vessels, and small-thickened interlobular septa. The “pomegranate sign” can be characterized as follows: the range of GGO further increases, the thickening of the interlobular septum become more significant, complicated with a small amount of punctate alveolar hemorrhage . The “rime sign” is characterized by numerous alveolar edema. Microscopically, the wall thickening, small vascular proliferation, luminal stenosis, and occlusion are seen, accompanied by interstitial infiltration of inflammatory cells, as well as numerous pulmonary interstitial fibrosis and partial hyaline degeneration. Repeated chest CT revealed the mediastinal lymphadenectasis in one patient. Re-examination of the NAT showed another positive anal swab in two patients . Conclusion: “Feather sign” and “dandelion sign” were typical chest CT features of COVID-19; “pomegranate sign” was an atypical feature, and “rime sign” was a severe feature . In clinical work, accurate identification of various chest CT signs in can help to improve the diagnostic accuracy of COVID-19 and reduce the misdiagnosis or missed diagnosis rate.
The Study on Serum Urate acid Target for Retarding Renal function to Worse in Diabetes
Authors: Ding Heyuan , Qian Yingjun , Wu Qing , Fan Weifeng , Niu Jianying , Gu Yong , Qin Qiaojing 
Published: June 2020

Abstract
Background: It’s unclear the exact level of serum urate acid (SUA) which can prevent renal function worse although all internists has been known the target of blood glucose, blood pressure and lipid in diabetes. It’s time to aim for a reasonable SUA target to prevent renal failure in diabetes.Methods: Using the physical examination results of 3427 diabetic patients in Jiangchuan Community, Shanghai, China, the relationship between SUA and estimated glomerular filtration rate (eGFR) decline was analyzed, and the appropriate cut-off point for SUA to predict eGFR decline was determined. Meanwhile, the population attributable risk proportion (PARP) of eGFR decline in diabetic patients above this SUA cut-off point was calculated. Results: eGFR decreased accompany with the increased SUA level and was negatively associated with the level of SUA significantly. After adjusting for potential confounders, SUA also was an independent risk factor of eGFR decline. The best appropriate SUA point, predicting eGFR decline obtained by ROC curve, was 326.5μmol/L which may prevent from eGFR decline in 33% male patients and 355.5μmol/L which may prevent from eGFR decline in 18% female patients. Compared with SUA>326.5μmol/L male and SUA>355.5μmol/L female group respectively, the relative risk of eGFR decline in SUA≤326.5μmol/L male and SUA≤355.5μmol/L female group is decreased significantly. Conclusions: SUA is an impotant risk factor for eGFR decline in diabetes. 326.5μmol/L in male and 355.5μmol/L in female may be used as the reasonable SUA target to retard renal function to worse in Chinese diabetes.
Chemokine Receptor Inhibitor vMIP-II Promoting Lymphocytes in COVID-19 Patients and Its Related Mechanism In Vitro
Authors: Li Shiyu , Wang Yuzhe , Feng Lixia , Jiang Zhenyou , Cong Feng , Chen Youyu , Dai Zhenning , Liu Shuting , Zhu Shitao , Fei Zhengbin , Xu Yichao , Mo Xuemei , Shi Shanshan , Zheng Xiao , Xu Anding , Gao Yong , Huang Wenhua , Zhang Nuofu , Sun Hanxiao 
Published: June 2020

Abstract
Coronavirus disease (COVID-19) accompanies severe immune injury as well as a decrease and overactivation of T lymphocytes. We observed that vMIP-Ⅱ, a broad-spectrum chemokine receptor inhibitor, could improve the lymphocyte decrease of COVID-19. Comparisons of T cell populations in PBMCs showed that the effects of vMIP-II on the subsets of T cells and cytokine secretion stimulated by SARS-CoV-2 S protein were the same as those in the asymptomatic infection group: the proportion of CD8+ TCM cells in the vMIP-II treatment and asymptomatic groups was significantly higher than that in the symptomatic control group. Differential gene expression of effector CD8+ T cells suggested that vMIP-II inhibits multiple chemokine receptors and related signal pathway and strengthens their stem proliferating capacity. Thus, vMIP-II reconstitutes cellular immunity lost due to acute infection of SARS-CoV-2 by modulating effector CD8+ T cells to produce more TCM cells.
A Novel Amplification-free SARS-CoV-2 Point-of-care Nucleic Acid Detection System based on Hybrid Capture Fluorescence Immunoassay
Authors: Wang Daming , He Shaogui , Wang Xiaohui , Chen Min , Li Li , Lei Yang , Zhang Jieli , Zhang Liwei , Hu Xiao , Zheng Xinhui , Bai Jiawei , Song Mingxuan , Tang Yuguo 
Published: June 2020

Abstract
Herein we presented a novel, rapid and amplification-free SARS-CoV-2 nucleic acid detection system based on hybrid capture fluorescence immunoassay (HC-FIA) technology. The usage of the monoclonal antibody S9.6 in recognizing DNA-RNA double-stranded hybrids enabled the conversion of nucleic acid testing into immunofluorescence carrying on a simple lateral flow dipstick. The established HC-FIA also exhibited satisfactory sensitivity, specificity and great robustness. The clinical evaluation of HC-FIA kit and fluorescence reading device are further processed in three hospitals independently. The results of 734 samples from 670 subjects indicated high consistency between our HC-FIA and quantitative polymerase chain reaction based commercially available kit or clinical diagnosis according to Kappa statistics. Altogether, HC-FIA related method and commercial test kit show unparalleled advantages as time saving, amplification-free, high throughput and portable POCT molecular diagnosis, which facilitates its application as on-site Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid detection in epidemic prevention and control worldwide, especially during the outbreak.
Development Strategy of Earthenware Craft Business Group with Analytic Network Process Approach
Authors: Ismail Khafid , Prajanti Sucihatiningsih Dian Wisika , Widiyanto Widiyanto 
Published: June 2020

Abstract This study aims to develop a strategy for developing Earthenware Craft Business Group in Kasongan Tourism Village. This research method uses mix methods, used namely primary and secondary data. The analytical method used is the Analytic Network Process (ANP) analysis. The results showed that the priority order of the first criteria was the aspect of sales with a value weight of 49.3 %%, followed by the business actor aspect with a value weight of 31%, and the aspects of business products with a value weight of 19.5%. The finding of this research is that the sales aspect is a priority aspect to be developed. From the final results the determination of priorities obtained three main priorities namely aspects of product innovation with a weight of 39.16%, partnership with a weight of 38.81% and training with a weight of 36.32%. Keywords: earthenware, business, kasongan, analytic network process Strategi Pengembangan Kelompok Usaha Kerajinan Gerabah Dengan Pendekatan Analytic Network Process AbstrakPenelitian ini bertujuan untuk menyusun strategi pengembangan kelompok usaha kerajinan gerabah di Desa Wisata Kasongan. Metode penelitian ini menggunakan pendekatan kualitatif dan kuantitatif. Jenis data yang digunakan ada dua yaitu data primer dan sekunder. Metode analisis yang digunakan adalah analisis Analytic Network Process (ANP). Hasil penelitian menunjukkan bahwa urutan prioritas dari kriteria tersebut yang pertama adalah aspek penjualan dengan bobot nilai sebesar 49,3%%, selanjutnya diikuti oleh aspek pelaku usaha dengan bobot nilai sebesar 31 %, dan aspek produk usaha dengan bobot nilai sebesar 19,5%. Temuan dari penelitian ini adalah aspek penjualan adalah aspek yang diprioritaskan untuk dikembangkan. Dari hasil akhir penentuan prioritas diperoleh tiga prioritas utama yaitu aspek inovasi produk dengan bobot 39,16%, kemitraan dengan bobot 38,81% dan pelatihan dengan bobot 36,32%. Kata kunci: gerabah, kasongan, kerajinan, pengembanganKeywords: Earthenware, Business, Kasongan, Analytic Network Process