bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Postglacial Isotopic Record of Intermediate Water Connects Mediterranean Sapropels and Organic‐Rich Layers
Authors: Incarbona Alessandro , Sprovieri Mario 
Published: September 2020

AbstractCarbon‐rich layers exist at both sides of the Mediterranean Sea sedimentary record and are called sapropels and organic rich layers (ORLs), respectively, in the eastern and western basins. They have different levels of organic carbon accumulation and seafloor oxygen deprivation. The most recent sapropel and ORL depositions have a different timing, approximately 10.8–6.1 and 14.5–9.0 ka, respectively. Here we investigate oxygen isotopic records of three foraminifera species that occupy different habitats within the Sicily Channel water column since ~12.0 ka, thus in the sill between the eastern and western Mediterranean basins. These data are ice volume corrected, to get information on water masses density variability, and are accompanied by benthic foraminifera δ13C measurements to establish Sicily Channel seafloor ventilation. Our results, and the comparison with other chronologically well‐constrained Mediterranean records, highlight the connection of the two subbasins due to monsoon activity. The end of the maximum Nile River flooding at ~9.2 ka, and eastern Mediterranean seafloor reventilation above 1,800–1,500 m depth at ~ 8.2 and 7.2 ka, left a clear signature in the intermediate water isotopic record of the Sicily Channel. Concurrently, the western Mediterranean deep water circulation experienced a significant recovery after a long period of slowdown. We argue that African monsoon weakening was transmitted into the western Mediterranean, through the intermediate layer of circulation, where deep water formation took place and brought oxygen to the seafloor.
EGFR activity addiction facilitates anti-ERBB based combination treatment of squamous bladder cancer
Authors: Rose Michael , Maurer Angela , Wirtz Julia , Bleilevens Andreas , Waldmann Tanja , Wenz Maximilian , Eyll Marie , Geelvink Mirja , Gereitzig Melanie , Rüchel Nadine , Denecke Bernd , Eltze Elke , Herrmann Edwin , Toma Marieta , Horst David , Grimm Tobias , Denzinger Stefan , Ecke Thorsten , Vögeli Thomas Alexander , Knuechel Ruth , Maurer Jochen , Gaisa Nadine T. 
Published: September 2020

AbstractRecent findings suggested a benefit of anti-EGFR therapy for basal-like muscle-invasive bladder cancer (MIBC). However, the impact on bladder cancer with substantial squamous differentiation (Sq-BLCA) and especially pure squamous cell carcinoma (SCC) remains unknown. Therefore, we comprehensively characterized pure and mixed Sq-BLCA (n = 125) on genetic and protein expression level, and performed functional pathway and drug-response analyses with cell line models and isolated primary SCC (p-SCC) cells of the human urinary bladder. We identified abundant EGFR expression in 95% of Sq-BLCA without evidence for activating EGFR mutations. Both SCaBER and p-SCC cells were sensitive to EGFR tyrosine kinase inhibitors (TKIs: erlotinib and gefitinib). Combined treatment with anti-EGFR TKIs and varying chemotherapeutics led to a concentration-dependent synergism in SCC cells according to the Chou-Talalay method. In addition, the siRNA knockdown of EGFR impaired SCaBER viability suggesting a putative “Achilles heel” of Sq-BLCA. The observed effects seem Sq-BLCA-specific since non-basal urothelial cancer cells were characterized by poor TKI sensitivity associated with a short-term feedback response potentially attenuating anti-tumor activity. Hence, our findings give further insights into a crucial, Sq-BLCA-specific role of the ERBB signaling pathway proposing improved effectiveness of anti-EGFR based regimens in combination with chemotherapeutics in squamous bladder cancers with wild-type EGFR-overexpression.
The Greatwall kinase safeguards the genome integrity by affecting the kinome activity in mitosis
Authors: Bisteau Xavier , Lee Joann , Srinivas Vinayaka , Lee Joanna H. S. , Niska-Blakie Joanna , Tan Gifford , Yap Shannon Y. X. , Hom Kevin W. , Wong Cheng Kit , Chae Jeongjun , Wang Loo Chien , Kim Jinho , Rancati Giulia , Sobota Radoslaw M. , Tan Chris S. H. , Kaldis Philipp 
Published: September 2020

AbstractProgression through mitosis is balanced by the timely regulation of phosphorylation and dephosphorylation events ensuring the correct segregation of chromosomes before cytokinesis. This balance is regulated by the opposing actions of CDK1 and PP2A, as well as the Greatwall kinase/MASTL. MASTL is commonly overexpressed in cancer, which makes it a potential therapeutic anticancer target. Loss ofMastlinduces multiple chromosomal errors that lead to the accumulation of micronuclei and multilobulated cells in mitosis. Our analyses revealed that loss ofMastlleads to chromosome breaks and abnormalities impairing correct segregation. Phospho-proteomic data forMastlknockout cells revealed alterations in proteins implicated in multiple processes during mitosis including double-strand DNA damage repair. In silico prediction of the kinases with affected activity unveiled NEK2 to be regulated in the absence ofMastl. We uncovered that, RAD51AP1, involved in regulation of homologous recombination, is phosphorylated by NEK2 and CDK1 but also efficiently dephosphorylated by PP2A/B55. Our results suggest thatMastlKOdisturbs the equilibrium of the mitotic phosphoproteome that leads to the disruption of DNA damage repair and triggers an accumulation of chromosome breaks even in noncancerous cells.
A human Myogenin promoter modified to be highly active in alveolar rhabdomyosarcoma drives an effective suicide gene therapy
Authors: Pruller Johanna , Hofer Isabella , Ganassi Massimo , Heher Philipp , Ma Michelle T. , Zammit Peter S. 
Published: September 2020

AbstractRhabdomyosarcoma is a rare childhood soft tissue cancer whose cells resemble poorly differentiated skeletal muscle, expressing myogenic proteins including MYOGENIN. Alveolar rhabdomyosarcoma (ARMS) accounts for ~40% of cases and is associated with a poorer prognosis than other rhabdomyosarcoma variants, especially if containing the chromosomal translocation generating the PAX3-FOXO1 hybrid transcription factor. Metastasis is commonly present at diagnosis, with a five-year survival rate of <30%, highlighting the need for novel therapeutic approaches. We designed a suicide gene therapy by generating an ARMS-targeted promoter to drive the herpes simplex virus thymidine kinase (HSV-TK) suicide gene. We modified the minimal human MYOGENIN promoter by deleting both the NF1 and MEF3 transcription factor binding motifs to produce a promoter that is highly active in ARMS cells. Our bespoke ARMS promoter driving HSV-TK efficiently killed ARMS cells in vitro, but not skeletal myoblasts. Using a xenograft mouse model, we also demonstrated that ARMS promoter-HSV-TK causes apoptosis of ARMS cells in vivo. Importantly, combining our suicide gene therapy with standard chemotherapy agents used in the treatment of rhabdomyosarcoma, reduced the effective drug dose, diminishing deleterious side effects/patient burden. This modified, highly ARMS-specific promoter could provide a new therapy option for this difficult-to-treat cancer.
Prevalence and risk factors of hypovitaminosis D in pregnant Spanish women
Authors: Díaz-López Andrés , Jardí Cristina , Villalobos Marcela , Serrat Nuria , Basora Josep , Arija Victoria 
Published: September 2020

AbstractThe hypovitaminosis D epidemic is a global health problem. Our aim was to assess the prevalence and potential risk factors of hypovitaminosis D among pregnant women on the eastern Mediterranean coast. Cross-sectional analysis involved 793 healthy pregnant women (35.3 ± 5.0 years) participating in ECLIPSES, a multicenter randomized trial. Socio-demographic, obstetric, anthropometric, lifestyle, dietary variables and blood draw was collected in the first trimester. Vitamin D deficiency was identified in 50.2% and insufficiency in 30.3% of pregnant women. The mean vitamin D level in the overall sample was 33.9 nmol/L (SD, 17.0). Multivariable logistic regression analysis applying AIC-based backward selection identified excess weight during the 1st trimester (BMI ≥ 25 kg/m2) (OR = 1.950, 95% CI = 1.409, 2.699), Arab ethnic group/dark skin colour (OR = 4.005, 95% CI = 2.488, 6.447), winter/spring (OR = 4.319, 95% CI = 3.112, 5.994), and consumption of milk (OR = 0.754, 95% CI = 0.572, 0.993) and yogurt (OR = 0.635, 95% CI = 0.436, 0.922) as independent risk factors for vitamin D deficiency. All of these factors (except yogurt consumption) and physical activity were independently associated with vitamin D deficiency/insufficiency risk in the final multivariable model (all p < 0.05). All these factors and social class were the most important determinants of circulating 25(OH)D concentrations. Our results confirm a high prevalence of vitamin D deficiency and insufficiency among pregnant women from the eastern Mediterranean coast.
Resting-state functional magnetic resonance imaging of high altitude patients with obstructive sleep apnoea hypopnoea syndrome
Authors: Qin Zongyuan , Kang Dongjie , Feng Xiang , Kong Demin , Wang Fangfang , Bao Haihua 
Published: September 2020

AbstractThe objective of the study was to observe brain function changes in Obstructive Sleep Apnoea Hypopnoea Syndrome (OSAHS) patients at high altitude. Resting-state functional magnetic resonance imaging (rs-fMRI) in patients with OSAHS was assessed using regional homogeneity (ReHo), amplitude of low frequency fluctuation (ALFF) and functional connectivity (FC). In this study, 36 male patients with OSAHS and 38 healthy male subjects were recruited from high-altitude areas, specifically, altitudes of 2,000–3,000 m. OSAHS was diagnosed by polysomnography (PSG). The blood oxygen level-dependent (BOLD) signals of OSAHS patients and healthy controls in the resting state were obtained and compared using ReHo, ALFF and FC methods. The posterior cingulate cortex (PCC) was selected as the seed region in the comparison of FC between the two groups. Compared with the healthy control group, multiple brain functions in the OSAHS patient group were different. There were correlations between the brain function values of some brain regions and demographic data. We also found that in contrast to earlier findings with individuals in plains areas, the brain function at the frontal lobe and the precuneus were higher in OSAHS patients, and the PCC showed higher FC with the left caudate, which may be due to the high-altitude hypoxic environment.
Answering Mermin’s challenge with conservation per no preferred reference frame
Authors: Stuckey W. M. , Silberstein Michael , McDevitt Timothy , Le T. D. 
Published: September 2020

AbstractIn 1981, Mermin published a now famous paper titled, “Bringing home the atomic world: Quantum mysteries for anybody” that Feynman called, “One of the most beautiful papers in physics that I know.” Therein, he presented the “Mermin device” that illustrates the conundrum of quantum entanglement per the Bell spin states for the “general reader.” He then challenged the “physicist reader” to explain the way the device works “in terms meaningful to a general reader struggling with the dilemma raised by the device.” Herein, we show how “conservation per no preferred reference frame (NPRF)” answers that challenge. In short, the explicit conservation that obtains for Alice and Bob’s Stern-Gerlach spin measurement outcomes in the same reference frame holds only on average in different reference frames, not on a trial-by-trial basis. This conservation is SO(3) invariant in the relevant symmetry plane in real space per the SU(2) invariance of its corresponding Bell spin state in Hilbert space. Since NPRF is also responsible for the postulates of special relativity, and therefore its counterintuitive aspects of time dilation and length contraction, we see that the symmetry group relating non-relativistic quantum mechanics and special relativity via their “mysteries” is the restricted Lorentz group.
In vivo assessment of the antischistosomal activity of curcumin loaded nanoparticles versus praziquantel in the treatment of Schistosoma mansoni
Authors: Mokbel Kamal El-Din M. , Baiuomy Ibrahim R. , Sabry Abd El-Hamid A. , Mohammed Mona M. , El-Dardiry Marwa A. 
Published: September 2020

AbstractSchistosomiasis is a serious parasitic infection affecting millions worldwide. This study aimed to explore the anti-schistosomal activity of curcumin and curcumin loaded gold-nanoparticles (Cur-GNPs) with or without praziquantel (PZQ). We used six groups of the C57BL/6 mice in which five groups were infected with Schistosoma Mansoni (S. mansoni) cercariae and exhibited, separately, to different treatment regimens of curcumin, curcumin loaded nanoparticle, and PZQ, in addition to one untreated group which acts as a control. Mice were sacrificed at the 8th week where both worms and eggs were counted in the hepatic and porto-mesenteric vessels in the liver and intestine, respectively, in addition to a histopathological examination of the liver granuloma. Curcumin caused a significant reduction in the worms and egg count (45.45%) at the 3rd week. A significant schistosomicidal effect of PZQ was found in all groups. Cur-GNPs combined with PZQ 97.4% reduction of worm burden in the 3rd week and the highest reduction in the intestinal and hepatic egg content, as well, besides 70.1% reduction of the granuloma size. The results suggested the curcumin in combination with PZQ as a strong schistosomicidal regimen against S. mansoni as it alters the hematological, biochemical, and immunological changes induced.
A machine learning-based diagnostic model associated with knee osteoarthritis severity
Authors: Kwon Soon Bin , Ku Yunseo , Han Hyuk-Soo , Lee Myung Chul , Kim Hee Chan , Ro Du Hyun 
Published: September 2020

AbstractKnee osteoarthritis (KOA) is characterized by pain and decreased gait function. We aimed to find KOA-related gait features based on patient reported outcome measures (PROMs) and develop regression models using machine learning algorithms to estimate KOA severity. The study included 375 volunteers with variable KOA grades. The severity of KOA was determined using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). WOMAC scores were used to classify disease severity into three groups. A total of 1087 features were extracted from the gait data. An ANOVA and student’s t-test were performed and only features that were significant were selected for inclusion in the machine learning algorithm. Three WOMAC subscales (physical function, pain and stiffness) were further divided into three classes. An ANOVA was performed to determine which selected features were significantly related to the subscales. Both linear regression models and a random forest regression was used to estimate patient the WOMAC scores. Forty-three features were selected based on ANOVA and student’s t-test results. The following number of features were selected from each joint: 12 from hip, 1 feature from pelvic, 17 features from knee, 9 features from ankle, 1 feature from foot, and 3 features from spatiotemporal parameters. A significance level of < 0.0001 and < 0.00003 was set for the ANOVA and t-test, respectively. The physical function, pain, and stiffness subscales were related to 41, 10, and 16 features, respectively. Linear regression models showed a correlation of 0.723 and the machine learning algorithm showed a correlation of 0.741. The severity of KOA was predicted by gait analysis features, which were incorporated to develop an objective estimation model for KOA severity. The identified features may serve as a tool to guide rehabilitation and progress assessments. In addition, the estimation model presented here suggests an approach for clinical application of gait analysis data for KOA evaluation.
Response of plant reflectance spectrum to simulated dust deposition and its estimation model
Authors: Zhu Jiyou , Zhang Xinna , He Weijun , Yan Xuemei , Yu Qiang , Xu Chengyang , Jiang Qun’ou , Huang Huaguo , Wang Ruirui 
Published: September 2020

AbstractTo quantitatively reflect the relationship between dust and plant spectral reflectance. Dust from different sources in the city were selected to simulate the spectral characteristics of leaf dust. Taking Euonymus japonicus as the research object. Prediction model of leaf dust deposition was established based on spectral parameters. Results showed that among the three different dust pollutants, the reflection spectrum has 6 main reflection peaks and 7 main absorption valleys in 350–2500 nm. A steep reflection platform appears in the 692–763 nm band. In 760–1400 nm, the spectral reflectance gradually decreases with the increase of leaf dust coverage, and the variation range was coal dust > cement dust > pure soil dust. The spectral reflectance in 680–740 nm gradually decreases with the increase of leaf dust coverage. In the near infrared band, the fluctuation amplitude and slope of its first derivative spectrum gradually decrease with the increase of leaf dust. The biggest amplitude of variation was cement dust. With the increase of dust retention, the red edge position generally moves towards short wave direction, and the red edge slope generally decreases. The blue edge position moved to the short wave direction first and then to the long side direction, while the blue edge slope generally shows a decreasing trend. The yellow edge position moved to the long wave direction first and then to the short wave direction (coal dust, cement dust), and generally moved to the long side direction (pure soil dust). The yellow edge slope increases first and then decreases. The R2 values of the determination coefficients of the dust deposition prediction model have reached significant levels, which indicated that there was a relatively stable correlation between the spectral reflectance and dust deposition. The best prediction model of leaf dust deposition was leaf water content index model (y = 1.5019x − 1.4791, R2 = 0.7091, RMSE = 0.9725).
Data-driven prediction and analysis of chaotic origami dynamics
Authors: Yasuda Hiromi , Yamaguchi Koshiro , Miyazawa Yasuhiro , Wiebe Richard , Raney Jordan R. , Yang Jinkyu 
Published: September 2020

AbstractAdvances in machine learning have revolutionized capabilities in applications ranging from natural language processing to marketing to health care. Recently, machine learning techniques have also been employed to learn physics, but one of the formidable challenges is to predict complex dynamics, particularly chaos. Here, we demonstrate the efficacy of quasi-recurrent neural networks in predicting extremely chaotic behavior in multistable origami structures. While machine learning is often viewed as a “black box”, we conduct hidden layer analysis to understand how the neural network can process not only periodic, but also chaotic data in an accurate manner. Our approach shows its effectiveness in characterizing and predicting chaotic dynamics in a noisy environment of vibrations without relying on a mathematical model of origami systems. Therefore, our method is fully data-driven and has the potential to be used for complex scenarios, such as the nonlinear dynamics of thin-walled structures and biological membrane systems.
LncRNA HAND2-AS1 inhibits proliferation and promotes apoptosis of non-small cell lung cancer cells by inactivating PI3K/Akt pathway
Authors: Gao Ting , Dai Xiaoqiang , Jiang Yindi , He Xiaopeng , Yuan Shuli , Zhao Peiwen 
Published: September 2020

Abstract
Background: Non-small cell lung cancer (NSCLC) is a major subtype of lung cancer and is correlated with high incidence and mortality rate. Functionality of lncRNA HAND2-AS1 is only reported in endometrioid endometrial carcinoma and osteosarcoma. In our study, the role of HAND2-AS1 in NSCLC was investigated.
Methods: We first detected the expression of HAND2-AS1 in lung tissues and serum of both NSCLC patients and healthy controls by qRT-PCR. Correlation between HAND2-AS1 expression level and clinical data of NSCLC patients was analyzed by Chi-square test. NSCLC cells, and cell proliferation, cell apoptosis and expression of PI3K/Akt pathway-related proteins were detected by CCK-8 assay, cell apoptosis assay and Western blot, respectively.
Results: HAND2-AS1 expression was significantly down-regulated in NSCLC. HAND2-AS1 and tumor size of NSCLC patients were closely associated. Serum HAND2-AS1 can be used to effectively distinguish osteosarcoma patients from healthy controls, and it can also be used to predict prognosis of osteosarcoma patients. HAND2-AS1 overexpression inhibited osteosarcoma cell proliferation, promoted cell apoptosis, and down-regulated phosphorylation of PI3K/Akt pathway-related proteins. PI3K/Akt pathway inhibitor showed no significant effects on HAND2-AS1 expression, but reduced its effects on cell proliferation and apoptosis.
Conclusion: We conclude that HAND2-AS1 may suppress the proliferation of NSCLC cells by targeting PI3K/Akt pathway.
Word Form Processing in Primary School Children: A Psycholinguistic Perspective
Authors: Hein Karin , Kauschke Christina 
Published: September 2020

Purpose
From a psycholinguistic perspective, the quality of the stored word form in the phonological input lexicon, as well as its effective retrieval from the phonological output lexicon, is of great importance in lexical processing. This study aimed at gaining a deeper understanding of (a)typical word form processing in primary school children. In particular, age-related development and profiles of word form processing including children's response behavior were investigated.

Method
A sample of 164 monolingual primary school children (6–9 years of age) was tested in a cross-sectional design using two word form–related tasks (auditory lexical decision and rapid naming) with carefully controlled stimuli in combination with traditional vocabulary tests and a nonword repetition task.

Results
First, an age-related improvement (better performance and acceleration of reaction times) was found for both word form–related tasks. Second, a cluster analysis revealed five clusters with different profiles of word processing. Beside a cluster with overall results of above average, we found two clusters including children with typical lexical abilities who applied specific strategies to deal with the tasks (speed–accuracy trade-offs). Two other clusters represented weak lexical abilities at different levels of word processing.

Conclusions
Children's abilities in word form processing develop over time and are characterized by developmental boosts that occur at different times in development. The uncovered profiles document patterns of (a)typical lexical processing. Since difficulties with word form processing are easily overlooked in clinical assessment, lexical decision and rapid naming tasks offer valuable tools for an in-depth evaluation of lexical skills.

Supplemental Material

https://doi.org/10.23641/asha.12986036