bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Facile, Sensitive and Rapid Sensing Platform Based on CoZnO for Detection of Fipronil; an Environmental Toxin
Authors: Kumar Sanni , Vasylieva Natalia , Singh Vikrant , Hammock Bruce , Singh Shiv Govind 
Published: June 2020

AbstractA sensitive detection of extremely toxic phenylpyrazole insecticide, ‘Fipronil’ is presented. Currently, the advancement of approaches for the detection of insecticides at low concentrations with less time is important for environmental safety assurance. Considering this fact, an effort has been made to develop an electrospun CoZnO nanofiber (NF) based label‐free electrochemical system for the detection of fipronil. The CoZnO NF were characterized using different techniques including field emission scanning electron microscopy (FE‐SEM), Energy Dispersive X‐Ray Analysis (EDX), X‐ray diffraction (XRD), Fourier‐transform infrared spectroscopy (FTIR), and Raman Spectroscopy. Based on the experimental results, the proposed platform displayed a linear response for fipronil in the attogram/mL range despite the multiple interfering agents. The sensitivity of the device was found to be 3.99 KΩ (g/ml)−1 cm−2. Limit of detection (LOD) and limit of quantification (LOQ) were calculated and found to be 112 ag mL−1 and 340 ag mL−1 respectively. Further, this proposed sensor will be implemented in the fields for the rapid and proficient detection of the real samples.
Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker
Authors: Xia Ya‐Mu , Li Meng‐Ying , Chen Cheng‐Long , Xia Meng , Zhang Wen , Gao Wei‐Wei 
Published: June 2020

AbstractA label‐free DNA biosensor based on three‐dimensional reduced graphene oxide (3D‐rGO) and polyaniline (PANI) nanofibers modified glassy carbon electrode (GCE) was successfully developed for supersensitive detection of breast cancer BRCA1. The results demonstrated that 3D‐rGO and PANI nanofibers had synergic effects for reducing the charge transfer resistance (Rct), meaning a huge enhancement in electrochemical activity of 3D‐rGO‐PANI/GCE. Probe DNA could be immobilized on 3D‐rGO‐PANI/GCE for special and sensitive recognition of target DNA (1.0×10−15–1.0×10−7 M) with a theoretical LOD of 3.01×10−16 M (3S/m). Furthermore, this proposed nano‐biosensor could directly detect BRCA1 in real blood samples.
Metal−Organic Frameworks toward Electrochemical Sensors: Challenges and Opportunities
Authors: Chuang Cheng‐Hsun , Kung Chung‐Wei 
Published: June 2020

AbstractOwing to their fascinating characteristics, metal−organic frameworks (MOFs) have attracted great attention and been utilized in a range of applications. The use of MOFs in electrochemical sensors has become an emerging subfield since 2013. However, the poor chemical stability in aqueous solutions and low electrical conductivity of most MOFs become two main concerns that hinder the use of pristine MOFs in electroanalytical systems. In this short review, we aim to focus on these issues and provide perspectives regarding the opportunities and possible strategies in future studies to overcome these challenges in order to design the MOF‐based electrochemical sensors.
Simultaneous determination of 20 bioactive components in Chuanxiong Rhizoma from different production origins in Sichuan province by ultra‐high‐performance liquid chromatography coupled with triple quadrupole mass spectrometry combined with multivariate statistical analysis
Authors: Wang Xiaoxiao , Yao Yixin , An Chang , Li Xi , Xiang Fangtao , Dong Yangli , Li Maosen 
Published: June 2020

AbstractChuanxiong Rhizoma is a commonly used in traditional Chinese medicine. Chuanxiong Rhizoma is widely distributed in Sichuan province, China, including the cities of Dujiangyan, Pengzhou, Meishan, Qionglai, and Shifang. However, reports on the comparisons of quality of Chuanxiong Rhizoma of different production origins are limited. Therefore, an ultra‐HPLC with triple quadrupole MS method was developed for the determination of 20 bioactive components (12 aromatic acids and eight phthalides) in 36 samples from different production origins and further assessed its quality. The contents of these 20 constituents of samples were analyzed by hierarchical cluster analysis and orthogonal partial least squares discrimination analysis; the result indicated that Chuanxiong Rhizoma of different production origins had some differences. Thirteen constituents of quality difference markers were acquired by variable importance for the project. Furthermore, the sum of the contents of these quality difference markers was different from various production origins of Chuanxiong Rhizoma. Meanwhile, Z‐ligustilide and senkyunolide A as main constituents of quality difference markers, the rate of various production origins of Chuanxiong Rhizoma was different. This study provides a foundation for the quality assessment of Chuanxiong Rhizoma.
A coplanar‐electrode direct‐current triboelectric nanogenerator with facile fabrication and stable output
Authors: Xu Guoqiang , Guan Dong , Yin Xing , Fu Jingjing , Wang Jie , Zi Yunlong 
Published: June 2020

AbstractRecently, the direct‐current (DC) TENG based on triboelectrification and discharge has been reported, which can not only generate a DC power, but also provide a higher charge density compared to traditional TENG. Here, we report a novel coplanar‐electrode DC TENG (CDC‐TENG) design which can be easily fabricated by two electrodes, and then a theoretical model is built to illustrate its principle and optimize its output performance. Both theoretical and experimental studies show that the designed device can enhance the energy output, and then the output power is optimized. Furthermore, we develop a three‐electrode CDC‐TENG to output DC during the reciprocating motion. We apply this CDC‐TENG design for rotational energy harvesting and produce a stable constant DC output. This represents an important progress to effectively store the energy harvested by the CDC‐TENG with the aim to drive portable/wearable/implantable electronics with the stable output.image
On the performance of WENO/TENO schemes to resolve turbulence in DNS/LES of high‐speed compressible flows
Authors: Hamzehloo Arash , Lusher David J. , Laizet Sylvain , Sandham Neil D. 
Published: June 2020

SummaryHigh‐speed compressible turbulent flows typically contain discontinuities and have been widely modeled using Weighted Essentially Non‐Oscillatory (WENO) schemes due to their high‐order accuracy and sharp shock capturing capability. However, such schemes may damp the small scales of turbulence and result in inaccurate solutions in the context of turbulence‐resolving simulations. In this connection, the recently developed Targeted Essentially Non‐Oscillatory (TENO) schemes, including adaptive variants, may offer significant improvements. The present study aims to quantify the potential of these new schemes for a fully turbulent supersonic flow. Specifically, DNS of a compressible turbulent channel flow with M = 1.5 and Reτ = 222 is conducted using OpenSBLI, a high‐order finite difference computational fluid dynamics framework. This flow configuration is chosen to decouple the effect of flow discontinuities and turbulence and focus on the capability of the aforementioned high‐order schemes to resolve turbulent structures. The effect of the spatial resolution in different directions and coarse grid implicit LES are also evaluated against the WALE LES model. The TENO schemes are found to exhibit significant performance improvements over the WENO schemes in terms of the accuracy of the statistics and the resolution of the three‐dimensional vortical structures. The sixth‐order adaptive TENO scheme is found to produce comparable results to those obtained with nondissipative fourth‐ and sixth‐order central schemes and reference data obtained with spectral methods. Although the most computationally expensive scheme, it is shown that this adaptive scheme can produce satisfactory results if used as an implicit LES model.
New cross‐linkable poly[bis(octafluoropentoxy) phosphazene] biomaterials: Synthesis, surface characterization, bacterial adhesion, and plasma coagulation responses
Authors: Xu Li‐Chong , Chen Chen , Zhu Jieru , Tang Meixian , Chen Andy , Allcock Harry R. , Siedlecki Christopher A. 
Published: June 2020

AbstractBiomaterial‐associated microbial infection and thrombosis represent major issues to the success of long‐term use of implantable blood‐contacting medical devices. The development of new poly[bis(octafluoropentoxy) phosphazene (OFP) biomaterials provides new routes for combatting microbial infection and thrombosis. However, the limited mechanical properties of OFP to date render them unsuitable for application in medical devices and inhibit any attempts at subsequent surface topography modification. In this study, we synthesized cross‐linkable OFPs (X‐OFPs) with the different degrees of cross‐linking in an effort to improve the mechanical properties. The results showed that the surface chemistry and surface topography of X‐OFPs do not change significantly, but the surface mechanical stiffness increased after cross‐linking. Atomic force microscopic phase images showed that the polymer phase separation structures changed due to cross‐linking. Experiments with three bacterial strains: Staphylococcal epidermidis, Staphylococcal aureus, and Pseudomonas aeruginosa showed that bacterial adhesion was significantly decreased on the OFP and X‐OFPs for both the pre‐cross‐linked and cross‐linked as compared to polyurethane biomaterials. Furthermore, bacterial adhesions were lower on X‐OFP surfaces than on pre‐cross‐linked materials, suggesting that mechanical stiffness is an important parameter influencing bacterial adhesion. Blood plasma coagulation responses revealed longer coagulation times for OFP and X‐OFP materials than on polyurethanes, indicating that the new cross‐linked OFPs are resistant to plasma coagulation compared to currently used polyurethane biomaterials.
Association of genetic variants in lncRNA GAS5/miR‐21/mTOR axis with risk and prognosis of coronary artery disease among a Chinese population
Authors: Li Hu , Liu Yingxue , Huang Jinyan , Liu Yu , Zhu Yufeng 
Published: June 2020

AbstractBackgroundAllowing for the significance of single nucleotide polymorphisms (SNPs) in reflecting disease risk, this investigation attempted to uncover whether SNPs situated in lncRNA GAS5/miR‐21/mTOR axis were associated with risk and prognosis of coronary heart disease (CHD) among a Chinese Han population.MethodsAltogether 436 patients with CHD were recruited as cases, and meanwhile, 471 healthy volunteers were included into the control group. Besides, SNPs ofGAS5/MIR‐21/mTORaxis were genotyped utilizing mass spectrometry. Chi‐square test was applied to figure out SNPs that were strongly associated with CHD risk and prognosis, and combined effects of SNPs and environmental parameters on CHD risk were evaluated through multifactor dimensionality reduction (MDR) model.ResultsSingle nucleotide polymorphisms ofGAS5(ie, rs2067079 and rs6790), MIR‐21 (ie, rs1292037), andmTOR(rs2295080, rs2536, and rs1034528) were associated with susceptibility to CHD, and also Gensini score change of patients with CHD (P < .05). MDR results further demonstrated that rs2067079 and rs2536 were strongly interactive in elevating CHD risk (P < .05), while smoking, rs6790 and rs2295080 showed powerful reciprocity in predicting Gensini score change of patients with CHD (P < .05).ConclusionSingle nucleotide polymorphisms of lncRNA GAS5/miR‐21/mTOR axis might interact with smoking to regulate CHD risk, which was conducive to diagnosis and prognostic anticipation of CHD.
Mesenchymal stem cell‐derived extracellular vesicles suppress the fibroblast proliferation by downregulating FZD6 expression in fibroblasts via micrRNA‐29b‐3p in idiopathic pulmonary fibrosis
Authors: Wan Xuan , Chen Shuyun , Fang Yan , Zuo Wei , Cui Jian , Xie Shiguang 
Published: June 2020

AbstractIdiopathic pulmonary fibrosis (IPF), a progressive and fatal lung disease, usually leads to an irreversible distortion of the pulmonary structure. The functional roles of bone marrow‐derived mesenchymal stem cells (BMSC)‐secreted extracellular vesicles (EVs) in fibroblasts have been implicated, yet their actions in the treatment of IPF are not fully understood. This study investigated the roles of BMSC‐derived EVs expressing miR‐29b‐3p in fibroblasts in IPF treatment. EVs derived from BMSCs were successfully isolated and could be internalized by pulmonary fibroblasts, and Cell Counting Kit‐8 (CCK‐8) and Transwell assay results identified that EVs inhibited the activation of fibroblast in IPF. miR‐29b‐3p, frizzled 6 (FZD6), α‐skeletal muscle actin (α‐SMA), and Collagen I expressions were examined, which revealed that miR‐29b‐3p was poorly expressed and FZD6, α‐SMA, and Collagen I were overexpressed in pulmonary tissues. Dual‐luciferase reporter assay results demonstrated that miR‐29b‐3p could inversely target FZD6 expression. The gain‐ and loss‐of‐function assays were conducted to determine regulatory effects of FZD6 and miR‐29b‐3p on IPF. CCK‐8 and Transwell assays results displayed that BMSCs‐derived EVs overexpressing miR‐29b‐3p contributed to inhibited pulmonary interstitial fibroblast proliferation, migration, invasion, and differentiation. Furthermore, the effects of BMSCs‐derived EVs overexpressing miR‐29b‐3p on IPF progression were assessed in vivo, which confirmed the repressive effects of BMSCs‐derived EVs overexpressing miR‐29b‐3p on IPF progression. Collectively, BMSCs‐derived EVs overexpressing miR‐29b‐3p relieve IPF through FZD6.
The effects of macrophage‐mediated inflammatory response to the donor site on long‐term retention of a fat graft in the recipient site in a mice model
Authors: Wang Zijue , Chen Yunzi , Zhu Shengqian , Chen Xinyao , Guan Jingyan , Yao Yao , Wang Xinhui , Li Ye , Lu Feng , Gao Jianhua , Dong Ziqing 
Published: June 2020

AbstractInflammatory responses mediated by macrophages play a role in tissue repair. However, it is unclear whether the repair in the donor site after liposuction would have any effects on fat graft retention in the recipient site. This study is designed to evaluate the effects of a macrophage‐mediated inflammatory response in donor sites on long‐term retention of fat grafting. In this study, mice were randomly divided into two groups. One underwent simulated liposuction, called the fat procurement plus grafting (Pro‐Grafting) group, and the other underwent sham surgery, called the fat grafting only (Grafting Only) group. The prepared fat (0.3 ml each) was engrafted and cellular events over a 90‐day period were assessed. We found macrophages were infiltrated into adipose tissue at the recipient site in the Grafting Only group within 7 days and the repair essentially completed within 30 days. By contrast, few macrophages infiltrated the recipient site in the Pro‐Grafting group within 7 days and the entire remodeling process took 30 days longer in the Pro‐Grafting than the Grafting Only group. Moreover, C‐reactive protein levels were immediately upregulated after surgery, and the inflammatory factors' expression was higher at the donor rather than the recipient site. However, the repair processes and the long‐term retention rate became normal when the adipose tissue was grafted after the donor site did not require macrophages for repair. Therefore, we suggest higher inflammatory factors promote macrophage infiltration and the adipose tissue regeneration process at the donor site. This process is delayed at the recipient site, which may affect long‐term retention of fat grafts.
Long noncoding RNA EPB41L4A‐AS1 functions as an oncogene by regulating the Rho/ROCK pathway in colorectal cancer
Authors: Bin Jie , Nie Shaolin , Tang Ziyuan , Kang Anding , Fu Zhongping , Hu Yingbin , Liao Qianjin , Xiong Wei , Zhou Yujuan , Tang Yanyan , Jiang Jiarui 
Published: June 2020

AbstractColorectal cancer (CRC) is one of the most common malignant tumors worldwide. In terms of cancer‐related death, colon cancer ranks second and third among men and women, respectively, and the incidence is increasing annually. Accumulating evidence have indicated that long noncoding RNA (lncRNA) plays an important role in tumorigenesis. In this study, we found that lncRNA EPB41L4A‐AS1 was highly expressed in CRC tissues and was associated with poor prognosis and tumor metastasis in patients with CRC. In vitro studies showed that the knockdown of EPB41L4A‐AS1 inhibited the proliferation, migration, invasion, and epithelial–mesenchymal transition of CRC cells. Mechanically, we found that EPB41L4A‐AS1 may participate in the development of CRC by activating the Rho/Rho‐associated protein kinase signaling pathway. Collectively, these results demonstrated that EPB41L4A‐AS1 can promote the proliferation, invasion, and migration of CRC, and it may be a novel biomarker for the diagnosis and targeted treatment of CRC.
Diagnostic utility of superb microvascular imaging in depiction of corkscrew collaterals in Buerger's disease
Authors: Nas Omer Fatih , Kandemirli Sedat Giray , Erdemli Gursel Basak , Bilgin Cem , Korkmaz Baris , Yolgosteren Atif , Inecikli Mehmet Fatih 
Published: June 2020

AbstractPurposeTo evaluate the corkscrew collaterals in Buerger's disease by superb microvascular imaging (SMI) and power Doppler ultrasonography (PDU).MethodsWe evaluated with SMI and PDU 14 patients with Buerger's disease in whom corkscrew collaterals had been identified on digital subtraction angiography (DSA). Corkscrew collaterals were classified on DSA and PDU based on their size and morphology.ResultsA total of 17 vascular regions of collateral vessel formation were assessed. Based on DSA classification, there were three cases of type I collaterals (arterial diameter of >2 mm with large helical pattern), seven cases of type III collaterals (arterial diameter of 1‐1.5 mm with small helical pattern), and seven cases of type IV collaterals (arterial diameter of <1 mm with tiny helical pattern). On PDU, all type I collaterals on DSA appeared as “large snake” images, all type III collaterals on DSA appeared as “small snake” images, and all type IV collaterals on DSA appeared as dots. SMI imaging, both in color and monochrome mode, provided superior demonstration of the continuity of the vessel of large or small “snake” images. In cases appearing as dot pattern on PDU, color SMI was able to show continuity of the flow signal as a helical pattern.DiscussionSMI is a promising new Doppler imaging technique that is superior to conventional power Doppler imaging in depiction and identification of corkscrew collaterals in Buerger's disease.
Structural and Functional Thalamic Changes in Parkinson's Disease With Mild Cognitive Impairment
Authors: Li Ming‐ge , He Jian‐feng , Liu Xin‐yun , Wang Zhen‐fu , Lou Xin , Ma Lin 
Published: June 2020

BackgroundThe thalamus is a key node of deep gray matter and previous studies have demonstrated that it is involved in the modulation of cognition.PurposeTo investigate the volume changes of the thalamus and its subregions and altered thalamus functional connectivity patterns in Parkinson's disease (PD) patients with and without mild cognitive impairment (MCI).Study TypeProspective.PopulationThirty‐three patients with MCI (PD‐MCI), 36 PD patients having no cognitive impairment (PD‐NCI), 21 healthy controls (HCs).Sequence3.0T MRI scanner; 3D T1‐weighted fast spoiled gradient recalled echo (3D T1‐FSPGR); resting‐state fMRIAssessmentVoxel‐based morphometry (VBM) was performed to calculate the volume of the thalamus and its subregions. The left and right total thalamus were considered seeds and seed‐based functional connectivity (FC) was analyzed. Additionally, correlations between volumes and cognitive performance and between FC values and cognitive performance were examined separately.Statistical TestAnalysis of covariance (ANCOVA); two‐sample t‐tests; partial correlation analysis.ResultsThe volumes of the total thalamus (PD‐MCI vs. PD‐NCI vs. HCs: 18.39 ± 1.67 vs. 19.63 ± 1.79 vs. 19.47 ± 1.35) and its subregions were significantly reduced in PD‐MCI as compared to PD‐NCI (total thalamus: P = 0.002) and HCs (total thalamus: P = 0.012). Compared with PD‐NCI, PD‐MCI showed increased FC between the thalamus and bilateral middle cingulate cortex and left posterior cingulate cortex, and decreased FC between thalamus and the left superior occipital gyrus, left cuneus, left precuneus, and left middle occipital gyrus. Volumes of thalamus and the subregions, as well as the FC of thalamus with the identified regions, were significantly correlated (P < 0.05, FDR‐corrected) with neuropsychological scores in PD patients.Data ConclusionWe noted volume loss and altered FC of thalamus in PD‐MCI patients, and these changes were correlated with global cognitive performance.Level of Evidence2Technical EfficiencyStage 2 J. Magn. Reson. Imaging 2020;52:1207–1215.
Stable isotope ratio analysis combined with inductively coupled plasma‐mass spectrometry for geographical discrimination between Italian and foreign saffron
Authors: Perini Matteo , Pianezze Silvia , Ziller Luca , Ferrante Marco , Ferella Francesco , Nisi Stefano , Foschi Martina , D'Archivio Angelo Antonio 
Published: June 2020

AbstractSeventy‐six samples of saffron were analysed through inductively coupled plasma‐mass spectrometry (ICP‐MS) and stable isotope ratio analysis. The dataset was formed by 67 samples harvested in different areas of Italy, Morocco and Iran, and nine samples purchased in the Italian market. For the first time, 42 elements and five stable isotopes (δ13C, δ15N, δ34S, δ2H and δ18O) were considered to carry out the discrimination of the samples on the basis of their geographical origin. Combined ICP‐MS and isotopic composition data turned out to be a useful tool for the geographical discrimination of saffron among predefined cultivation sites.K, Cr, Mn, Ni, Zn, Rb, Sr, Mo, Cs, Nd, Eu, Pb, δ13C, δ15N, δ34S and δ2H were identified as the significant variables in geographical discrimination. Moreover, the class models generated for saffron cultivated in two specific areas of Central Italy exhibited 100% specificity for Moroccan, Iranian and commercial samples and a high specificity (83% and 84%) for the saffron samples cultivated in other, although close, Italian sites.
Age‐related hearing loss: Why we need to think about sex as a biological variable
Authors: Nolan Lisa S. 
Published: June 2020

AbstractIt has long been known that age‐related hearing loss (ARHL) is more common, more severe, and with an earlier onset in men compared to women. Even in the absence of confounding factors such as noise exposure, these sexdifferences in susceptibility to ARHL remain. In the last decade, insight into the pleiotrophic nature by which estrogen signaling can impact multiple signaling mechanisms to mediate downstream changes in gene expression and/or elicit rapid changes in cellular function has rapidly gathered pace, and a role for estrogen signaling in the biological pathways that confer neuroprotection is becoming undeniable. Here I review the evidence why we need to consider sex as a biological variable (SABV) when investigating the etiology of ARHL. Loss of auditory function with aging is frequency‐specific and modulated by SABV. Evidence also suggests that differences in cochlear physiology between women and men are already present from birth. Understanding the molecular basis of these sex differences in ARHL will accelerate the development of precision medicine therapies for ARHL.