bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Study on the Molecular Mechanism of Erchen Tang on Asthma Based on Network Pharmacology
Authors: Ren Yuzhe , Zhang Haijing , Yu Zhou , Wan Jun , Peng Xuejin , Huang Yilin , Li Chengke , Yang Xiangzheng 
Published: June 2020

Abstract
Objective: To explore the active ingredients of ECT and their targets of asthma and investigate the potential mechanism of ECT on asthma. Methods: Firstly, the active ingredients and target of ECT were screened for BATMAN and TCMSP, functional analysis was finished via DAVID. Then, animal model was induced by ovalbumin (OVA) and aluminum hydroxide. Eosinophil (EOS) counts, EOS active substance Eosinophilic cationic protein (ECP) and eotaxin levels were detected followed the instruction. Pathological changes of lungs tissue were examined by H&E staining and transmission electron microscopy. Interleukin (IL-4, IL-10, IL-13, TNF-α), TIgE and IgE level in bronchoalveolar lavage fluid (BALF) were measured by ELISA. Finally, the protein expression of TGF-β / STAT3 pathway to lung tissue was detected by Western Blot. Results: A total of 450 compounds and 526 target genes were retrieved in Erchen tang. Functional analysis indicated that its treatment of asthma was associated with inflammatory factor and fibrosis. In the animal experiment, the results showed that ECT significantly regulated inflammatory cytokine (IL-4, IL-10, IL-13, TNF-α) levels in (P<0.05, P<0.01) , reduced EOS number (P<0.05) and also ECP and Eotaxin levels in blood (P<0.05) in BALF and / or plasma. Bronchial tissue injury was obviously improved on ECT treatment. Associated protein in TGF-β / STAT3 pathway were significantly regulated by ECT (P<0.05). Conclusion: This study originally provided the evidence that the Erchen tang was effective against the treatment of asthma symptoms, and its underlying mechanism might be regulation of inflammatory factor secretion and TGF-β/STAT3 signaling pathway.
Analysis and finding the correlation of air quality parameters on the spread and deceased case of COVID-19 patients in India
Authors: Saha Bibek , Debnath Animesh , Saha Biswajit 
Published: June 2020

Abstract
Recent covid-19 pandemic state across the globe has brought some issues to be considered obviously for better sustainability of human society and economy. In this article a comprehensive study based on the air quality parameters has been represented in view the recent covid-19 situation in India. India being the second largest populated country in the world and having a very high population density is a vital region for controlling and observing the spread and recovery of covid-19 patients. It has been observed that the spread as well as recovery rate in India is far better than many other countries in the world. A noteworthy observation has been extracted in this study from the correlation between three Covid-19 parameters (total confirmed, active and deceased cases) and air quality index comprising of six air quality parameters (PM 2.5, PM 10, NO2, SO2, CO, and Ozone) of 25 highly Covid-19 affected Indian cities. Spearman rank correlation and Kendall correlation tests were used to understand the correlation between the Covid-19 variables with air quality variables. Both the statistical correlation test revealed that particulate matters like PM 2.5, PM 10, and overall air quality have significantly strong correlation with the Covid-19 spread and death cases in 25 cities of India. However, the PM 2.5 is found to be the most significant air quality parameter for Covid-19 spread and death cases in India. It indicates that the western part of India contains more risk zones as compared to the north-eastern and eastern part of the country. The high natural vegetation, less industrial areas of the northeast India with better air quality finds higher recovery rate. Similarly the high natural vegetation of the Himalayan zones are also comparatively having better air quality and experiencing better control of covid-19 cases.
Association of Neuronal Injury Blood Marker Neurofilament light chain with mild-to-moderate COVID-19
Authors: Ameres Markus , Brandstetter Susanne , Toncheva Antoaneta A. , Kabesch Michael , Leppert David , Kuhle Jens , Wellmann Sven 
Published: June 2020

Abstract
The coronavirus disease 2019 (COVID-19) affects primarily the respiratory system but neurologic manifestations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are possible. Serum neurofilament light chain (sNfL) has recently been considered as a specific biomarker to quantitate neuro-axonal damage. Concentrations of sNfL were determined in a prospective cohort study of 100 health care workers (84 females, 16 males) following a COVID-19 outbreak in a large hospital by using the single molecule array (Simoa) NF-light advantage kit. Twenty eight health care workers contracted mild-to-moderate COVID-19, recovered after 1-3 weeks without hospitalization and showed no or only minor neurological symptoms such as anosmia, fatigue or headache. sNfL levels were consistently higher in older persons and multivariable linear regression analysis revealed COVID-19 status as an independent predictor of sNfL (p=.005). In conclusion, increased sNfL levels in mild-to-moderate COVID-19 patients points towards a more general neuro-destructive capability of SARS-CoV-2.
The relationship between 25-hydroxyvitamin D concentration and liver enzymes in healthy adults: A Cross-sectional Study
Authors: Wang Ji-nian , Li Li , Hu Hua-qing , Yan Qi , Liu Ying , Cheng Shu-qi , Lian Pan-pan , Pan Faming , Xu Tao 
Published: June 2020

Abstract
Background

Studies have shown that serum 25-hydroxyvitamin D (25(OH)D), a product of exogenous vitamin D, plays an influential role in calcium and phosphorus balance, anti-inflammation, and cardiovascular protection. There were long-standing interests in the potential benefits of vitamin D for preventing liver diseases. Investigations showed that 25(OH)D was increased in adolescents with abnormal liver function. Yet data about the relationship of 25(OH)D concentration and liver enzymes from prospective cross-sectional study was limited. The present study is undertaken to examine whether serum 25-hydroxyvitamin D and glutamyl transferase (GGT), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were closely related.

Methods

The cross-sectional study of 368 healthy volunteers with no history of liver diseases was used to examine risk factors. Pearson correlation (or Spearman correlation for skewed variables) was performed to assess associations between GGT, ALT, and ALP and 25(OH)D. Linear regression analysis was conducted with GGT, ALT, and ALP as dependent variables in separate models.

Results

The results found no significant difference between ALT and ALP in different vitamin D groups (25(OH)D < 25 nmol/L、25-50 nmol/L and > 50 nmol/L) (Kruskal-Wallis test, all p > 0.05). In multiple linear regression analysis, GGT, ALT and ALP levels remained unrelated to 25(OH)D concentration after adjusting covariates including age, BMI, sex, BuN, Cr, UA, AST, Ghb, ALB, WBC, cholesterol, HDL, LDL, cholesterol, and total protein. (All p > 0.1).

Conclusion

This study suggested that there was no association between the 25(OH)D concentration and the levels of GGT, ALT or ALP in normal population.
Spatial-temporal Distribution and Demography of Knowlesi Malaria in Peninsular Malaysia, 2011 to 2018
Authors: Phang Wei Kit , Hamid Mohd Hafizi bin Abdul , Jelip Jenarun , Mudin Rose Nani binti , Chuang Ting-Wu , Lau Yee Ling , Fong Mun Yik 
Published: June 2020

Abstract
Background: The life-threatening zoonotic malaria caused by Plasmodium knowlesi is on the rise in Malaysia; however, previous studies mainly focus on the transmission in Malaysian Borneo. This study aims to describe the basic epidemiological characteristics of P. knowlesi infection and identify spatial clustering of knowlesi malaria in Peninsular Malaysia.
Methods : The spatial distribution of P. knowlesi monoinfection incidence was mapped across Peninsular Malaysia using Geographic Information System (GIS) approach. Demographic characteristics of the infection was investigated. Global and Local Moran’s I were used to analyze spatial autocorrelation and define clustering of knowlesi malaria incidence.
Results : Gua Musang and Lipis maintained the highest incidence rate (IR) as compared to other districts. Spatial analysis revealed that high IRs (hotspots) were clustered in the central-northern region of Peninsular Malaysia. In the demographic aspect, knowlesi malaria was more prevalent in male and age between 20 and 39.
Conclusions : This study revealed the spatial and temporal patterns of P. knowlesi in Peninsular Malaysia throughout 2011 to 2018. Knowlesi malaria control strategy should be emphasized in Malaysia malaria eradication program.
Transcription Factor Runx1 Activates Opn to Promote Tumor Progression in Head and Neck Cancer
Authors: Liu Kai , Hu Huiying , Jiang Huanyu , Zhang Haidong , Gong Shanchun , yu zhenkun 
Published: June 2020

Abstract
Background:Metastatic progression remains a major burden for head and neck squamous cell carcinoma (HNSCC). Runt-related transcription factor 1 (RUNX1)has been reported to be associated with an aggressive phenotype in several cancers. However, the precise roles of RUNX1 underlying the metastaticprogression of HNSCC remain largely unknown.Methods:RUNX1 expression levels in HNSCC cells and tissues were detected by quantitative real-time PCR (qPCR), Western blottingand immunohistochemistry (IHC). In vitro and in vivo assays were performed to investigate the function of RUNX1 in the metastatic phenotype and the tumorigenic capability of HNSCC cells. Luciferase reporter and chromatin immunoprecipitation (ChIP)-qPCR assays were performed to determine the underlying mechanism of RUNX1-mediated HNSCC aggressiveness.Results:RUNX1 was increased with disease progression in patients withHNSCC.Furthermore, we found that silencing ofRUNX1 significantly decelerated the malignant progression of HNSCC cells and reduced Osteopontin (OPN) expression in vitro, and weakened the tumorigenicityof HNSCC cells in vivo. Mechanistically, we demonstrated that RUNX1 played an important role in activating MAPK signaling by directly binding to the promoter of OPN.Conclusions: Our results provide new insight into the mechanisms underlying the facilitate metastasisability of RUNX1and reveal the therapeutic potential of targeting RUNX1 in HNSCC.
KRT23 Acts as an Oncogene in Hepatocellular Carcinoma by Regulating P21 via PI3K/AKT/GSK3β Pathway
Authors: Guo Dan , Ma Wenhui , Wang Ruhua , Li Yarui , Chowdhury Abu Taiub mohammed Mohiuddin , Ren Mudan , Lu Xinlan , He Shuixiang , Lu Guifang 
Published: June 2020

Abstract
Background:Hepatocellular carcinoma (HCC) is a leading cancer worldwide for which diagnosis, treatment and progression are largely unknown. Keratin23 is a potential biomarker forHCC development; however, regulatory mechanisms underlying its expression remain unclear. Inthis research we explored the expression and effect of KRT23 underlying HCC development. Materials and methods:GEPIA was applied to analyze the expression of KRT23 in HCC samples and Kaplan-Merier survival analysis for patients’ prognosis. Next, IHC was further conducted for confirming its expression in HCC tissues. Meanwhile qRT-PCR and western blot analysis were applied to examine the expression of KRT23 on both mRNA and protein level in HCC cell lines compared with immortal hepatocyte LO2. Cell experiments including MTT assay, apoptosis analysis, cell cycle assay and clone formation assay were conducted for cell proliferation while transwell assay and scratch test for metastasis in vitro. Moreover, xenograft tumors in nude mice were further conducted for verification in vivo. As for mechanism in depth, immunofluorescence and western blot were operated to explore the effect of KRT23 on EMT and PI3K/AKT/GSK3βsignaling pathway. Furthermore, Co-immunoprecipitation was applied for interaction between KRT23 and P21. Functional rescue experiments were conducted to further testify their mutual effect.Results:For this research, we discovered the high expression of KRT23 in HCC samples and cell lines. Functionally, KRT23 knockdown reduced cell proliferation and metastasis in vitro and vivo. Furthermore, KRT23 participated in EMT progression and interacted with P21 to mediate PI3K/AKT/GSK3βpathway in HCC development.Conclusion:To summarize, KRT23 accelerated HCC proliferation and metastasis by regulating P21 via PI3K/AKT/GSK3βpathway.
Vibrionaceae core and shell genes are non-randomly distributed into spatially distinct intracellular domains
Authors: Bækkedal Cecilie Sonnenberg , Kahlke Tim , Haugen Peik 
Published: June 2020

Abstract
Background: The genome of Vibrionaceae bacteria, which consists of two circular chromosomes, is replicated in a highly ordered fashion. In fast-growing bacteria, multifork replication results in higher gene copy numbers and increased expression of genes located close to the origin of replication of Chr 1 (ori1 ). This is believed to be a growth optimization strategy to satisfy the high demand of essential growth factors during fast growth. The relationship between ori1 -proximate growth-related genes and gene expression during fast growth has been investigated by many researchers. However, it remains unclear which other gene categories that are present close to ori1 and if expression of all ori1 -proximate genes is increased during fast growth, or if expression is selectively elevated for certain gene categories. Results: We calculated the pangenome of all complete genomes from the Vibrionaceae family and mapped the four pangene categories, core, softcore, shell and cloud, to their chromosomal positions. This revealed that core and softcore genes were found heavily biased towards ori1 , while shell genes were overrepresented at the opposite part of Chr 1 (i.e., close to ter1 ). RNA-seq of Aliivibrio salmonicida and Vibrio natriegens showed global gene expression patterns that consistently correlated with chromosomal distance to ori1 . Despite a biased gene distribution pattern, all pangene categories contributed to a skewed expression pattern at fast-growing conditions, whereas at slow-growing conditions, softcore, shell and cloud genes were responsible for elevated expression. Conclusion: The pangene categories were non-randomly organized on the two chromosomes, with an overrepresentation of core and softcore genes around ori1 . We mapped our gene distribution data on to the intracellular positioning of chromatin described for V. cholerae , and suggested that core/softcore and shell/cloud genes were enriched at two spatially separated intracellular regions in the cell. The concurrence of the spatial distribution of core and the level of gene expression in one intracellular region, implied that there is a link between the structural organization of core genes and their cellular function in the cell.
The Hardy Cross method and its implementation in Spain
Authors: Pons Josep Maria 
Published: June 2020

In May 1930, Hardy Cross (1885-1959) published an article called ‘Analysis of continuous frames by distributing fixed-end moments’ in the American Society of Civil Engineers (ASCE). This article proposed a new approach to Structural Theory, and its relevance could be compared to that of the Three Moments Theorem (also known as the Clapeyron Theorem). The Cross method, as this calculation methodology has been often called, had remarkable significance from the moment it came out until the 70s, when new calculation methods became popular.
In the present article, we will be trying to evaluate its impact in locations far from its origins; in particular, how it was understood and formulated in Spain. As will be demonstrated, the importance of this method was extremely relevant for theconstruction of new buildings and the implementation of new industries, which started to appear in a decisive moment for the development of the country. Even though the Hardy Cross method was the most widely used methodology at the time, two other procedures were also available; namely, the Kani and the Takabeya methods, methods that would also appear in the technical bibliography of the time. Despite the infrequent implementation of these other methods, we have briefly referred to both of them in the present paper. This article aims to show the relevance of the Cross method as well as its early implementation in Spain, by using academic bibliography of that time.
Lifestyle Segmentation to Explain the Online Health Information–Seeking Behavior of Older Adults: Representative Telephone Survey
Authors: Weber Winja , Reinhardt Anne , Rossmann Constanze 
Published: June 2020

BackgroundAs a result of demographic changes, the number of people aged 60 years and older has been increasing steadily. Therefore, older adults have become more important as a target group for health communication efforts. Various studies show that online health information sources have gained importance among younger adults, but we know little about the health-related internet use of senior citizens in general and in particular about the variables explaining their online health-related information–seeking behavior. Media use studies indicate that in addition to sociodemographic variables, lifestyle factors might play a role in this context.ObjectiveThe aim of this study was to examine older people’s health-related internet use. Our study focused on the explanatory potential of lifestyle types over and above sociodemographic variables to predict older adults’ internet use for health information.MethodsA telephone survey was conducted with a random sample of German adults aged 60 years and older (n=701) that was quota-allocated by gender, age, educational status, and degree of urbanity of their place of residence.ResultsThe results revealed that participants used the internet infrequently (mean 1.82 [SD 1.07]), and medical personnel (mean 2.89 [SD 1.11]), family and friends (mean 2.86 [SD 1.21]), and health brochures (mean 2.85 [SD 1.21]) were their main sources of health information. A hierarchical cluster analysis based on values, interests, and leisure time activities revealed three different lifestyle types for adults aged over 60 years: the Sociable Adventurer, the Average Family Person, and the Uninterested Inactive. After adding these types as second-step predictors in a hierarchical regression model with sociodemographic variables (step 1), the explained variance increased significantly (R2=.02, P=.001), indicating that the Average Family Person and the Sociable Adventurer use the internet more often for health information than the Uninterested Inactive, over and above their sociodemographic attributes.ConclusionsOur findings indicate that the internet still plays only a minor role in the health information–seeking behavior of older German adults. Nevertheless, there are subgroups including younger, more active, down-to-earth and family-oriented males that may be reached with online health information. Our findings suggest that lifestyle types should be taken into account when predicting health-related internet use behavior.
Electronic Mental Health as an Option for Egyptian Psychiatry: Cross-Sectional Study
Authors: Kamel Mostafa Mamdouh , Westenberg Jean Nicolas , Choi Fiona , Tabi Katarina , Badawy Adel , Ramy Hisham , Elsawi Hossam , Krausz Michael 
Published: June 2020

Background
Egypt is a country of nearly 100 million citizens, and there are less than 1000 registered psychiatrists. The mental health care system is under resourced and nearly inaccessible for the majority of the population. In addition, youth under the age of 25 years represent 50% of Egyptian citizens; however, there are no specific services addressing their unique needs. How can the needs of the largest population in the Middle East be effectively addressed? Is a web-based framework an option for Egyptian psychiatrists to serve the population?

Objective
The aims of this study were to better understand the opinions of psychiatrists on the current state of mental health care services in Egypt and their current knowledge on electronic mental health (EMH); assess the attitudes of Egyptian psychiatrists toward web-based interventions and telemedicine for mental health; and identify perceived advantages and barriers of EMH development in Egypt.

Methods
A cross-sectional survey was conducted online among 640 Egyptian psychiatrists. It included a total of 36 items within a set of 16 questions asking about EMH literacy, integrating EMH into the mental health care system, and the perceived priorities and barriers of EMH. The sampling was supported by Tanta University, a large academic institution close to Cairo. Statistical analysis was performed using SPSS 25 (IBM Corp). Descriptive statistics, the chi-square test, the independent sample t test, and analysis of variance were applied.

Results
A total of 188 participants responded (response rate of 29.4%), of which 54.2% (102/188) were female and 54.3% (102/188) were between 30 and 45 years old. Less than half of the participants thought that the current health care system was efficient for adults (69/155, 44.4%), and even less thought it was efficient for youth (44/155, 28.3%). Almost all participants agreed that EMH would be beneficial for patient care (147/155, 94.8%) and that integrating EMH into the current health care system would be a good idea (118/155, 76.2%). The highest rated utility of web-based solutions was documentation, followed by psychoeducation and communication with professionals. The main advantages were to improve access to care in rural areas of the country and its convenience.

Conclusions
There is scarcity of mental health resources in Egypt. Egyptian psychiatrists are interested in EMH and believe web-based platforms can become part of the solution for the Egyptian mental health care system.
The Influence Of Health Education On The Selection Of Healthy Snacks For School
Authors: Putri Ronasari Mahaji 
Published: June 2020

Usia sekolah merupakan masa pertumbuhan dan perkembangan anak.Asupan makan yang baik dan bergizi akan sangat menunjang pertumbuhan dan perkembangan anak usia sekolah. Jajanan bisa dianggap sebagai salah satu kebutuhan anak sekolah yang harus dipenuhi. Pemilihan jajan anak yang tidak sehat berdampak pada kesehatan anak. Tujuan penelitian untuk mengetahui pengaruh pendidikan pendidikan kesehatan terhadap pemilihan jajan sehat anak sekolah.Merupakan penelitian quasy eksperiment dengan the one group pre test post test design. Populasi adalah seluruh anak SD Merjosari 3 Malang sejumlah 196 orang. Sampel sejumlah 69 anak diambil melalui purposive sampling. Instrumen dalam penelitian ini adalah kuesioner. Analisa data menggunakan uji t test 2 sampel berpasangan. Hasil penelitian diketahui pengetahuan pemilihan jajan anak meningkat setelah pemberian pendidikan kesehatan dari kategori cukup ( 54,2%) menjadi baik (47%).Hasil uji statistic menunjukkan bahwa ada pengaruh pendidikan kesehatan terhadap pemilihan jajan anak di SDN 3 Merjosari Malang (pvalue 0,00) dengan kekutan hubungan 0,912