bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improvement on high-cholesterol diet induced atherosclerosis, lipid profile, oxidative stress and genotoxicity in the liver of mice by Echinops spinosissimus Turra subsp. spinosus
Authors: Dammak Donyez Frikha , Saad Hajer Ben , Bouattour Emna , Boudawara Ons , Jarraya Raoudha Mezghani 
Published: March 2020

Abstract
Background Hypercholesterolemia is a major risk factor for the development of atherosclerosis and endothelial dysfunction. Methods The present study investigates the possible mechanism of Echinops spinosissimus Turra subsp. spinosus ( E. s. spinosus ) flower on the high cholesterol diet. Results Our in vitro results demonstrated the richness of E.s. spinosus flower in antioxidant compounds, and its antioxidant activities. The co-administration of E.s. spinosus (100 or 200 mg/kg/day) with high-fat diet attenuated hepatotoxicity as monitored by the improvement of oxidative stress biomarkers and plasma lipid and liver parameters, when compared to the hypercholesterolemic mice. Atherogenic index and body weight were also reduced markedly, compared to control mice. These results were confirmed by the improvement of histological changes and DNA damage. Conclusion These data indicate that E.s. spinosus flower reduces the hypercholesterolemia risk and atherogenic properties of dietary cholesterol. Its hypocholesterolemic effect may be due to its antioxidant activities and its richness in bioactive molecule.
Failure to replicate the JTC-bias in patients with psychotic disorders: A secondary analysis of a baseline assessment of a randomized controlled trial
Authors: Pytlik Nico , Soll Daniel , Hesse Klaus , Moritz Steffen , Bechdolf Andreas , Herrlich Jutta , Kircher Tilo , Klingberg Stefan , Landsberg Martin W. , Müller Bernhard W. , Wiedemann Georg , Wittorf Andreas , Wölwer Wolfgang , Wagner Michael , Mehl Stephanie 
Published: March 2020

Abstract
Background: The jumping to conclusions bias (JTC) is considered to be an important causal factor in theoretical models on the formation and maintenance of delusions. However, recent meta-analytic findings show a rather equivocal pattern of results regarding associations between JTC and delusions. Thus, the aim of the present preregistered study is to investigate in a large sample whether the JTC-bias is more pronounced in patients with psychotic disorders in comparison to controls and whether the JTC bias is associated with more severe delusional conviction, persecutory delusions and positive symptoms in general. All hypotheses were pre-specified in the trial proposal.Methods: Patients with psychotic disorders (n = 300) enrolled in a therapy trial and healthy controls (n = 51) conducted a variant of the beads task (fish task) as a measure for the JTC-bias at start of the trial. Further, clinical interviews were used to assess patients’ delusional severity and delusional conviction.Results: There were no statistically significant differences between patients with psychotic disorders (with 53% displaying the JTC-bias) and controls (41%). Furthermore, there were no statistically significant correlations between JTC measures and persecutory delusions, delusional conviction and positive symptoms.Conclusions: We found no evidence for hasty decisions in patients with psychotic disorders, possibly because the fish task induces a more deliberative reasoning style as compared to the traditional beads task. The lack of associations between JTC measures and delusion severity is in line with meta-analytic findings using a wide range of JTC task variants. JTC is not as ubiquitous as previously thought and may only manifest itself in certain situational contexts. Trial Registration: ISRCTN29242879 (isrctn.com), date of registration: April 12th 2006, http://www.isrctn.com/ISRCTN12345678
Simplified Minimally Invasive Beating Heart Technique for Redo Isolated Tricuspid Valve Surgery
Authors: Lu Shuyang , Song Kai , Yao Wangchao , Xia Limin , Dong Lili , Sun Yongxin , Hong Tao , Yang Shouguo , Wang Chunsheng 
Published: March 2020

Abstract
BackgroundRedo isolated tricuspid valve surgery has been associated with a high morbidity and mortality, and its optimal timing of surgical intervention remains controversial. Hence, we reviewed our early and midterm results with a simplified minimally invasive beating heart technique for isolated redo tricuspid valve surgery in patients at high risk.MethodsBetween June 2016 and August 2017, a total of 14 consecutive patients underwent isolated tricuspid valve operations after previous cardiac operations with minimally invasive beating heart technique through a right lateral thoracotomy in our center. Mean patient age was 54.0 ± 8.3 years, and 9 patients (64.3%) were women. Mean preoperative EuroSCORE was 8.1 ± 1.3 (6 to 11). Previous cardiac operations included 6 patients (42.9%) with mitral valve replacement, 1 patient (7.1%) with mitral valve replacement and tricuspid valve repair, 1 patient (7.1%) with tricuspid valve replacement, 5 patients (35.7%) with mitral valve and aortic valve replacement, and 1 patient (7.1%) with Ebstein repair. Midterm follow-up was complete for 12 patients (85.7%).ResultsBoth in-hospital and thirty-day mortalities were 0%. Tricuspid valve replacement with bioprosthesis was performed in 12 patients (85.7%), and the remaining 2 patients (14.3%) underwent tricuspid repair (annuloplasty and leaflets reconstruction). Mean cardiopulmonary bypass time was 55.6 ± 10.7 minutes. Overall in-hospital duration and intensive care unit (ICU) time were 11.6 ± 8.8 days, 3.9 ± 2.8 days, respectively. Postoperative complications included 2 patients (1.4%) with prolonged ventilation, and 2 patients (1.4%) with acute kidney injury. There were no postoperative cerebrovascular accidents, myocardial infarctions, reoperations for bleeding, or deep wound infections. All patients were discharged uneventful. Except 2 patients lost follow-up, there were no adverse cardiovascular events and deaths occurred in other patients.ConclusionsSimplified minimally invasive beating heart technique for redo tricuspid valve surgery is both feasible and safe, and the early and midterm results are excellent.
Construction and analysis of the lncRNA-miRNA-mRNA network based on competitive endogenous RNA reveal functional lncRNAs in oral cancer
Authors: Yin Junhao , Zeng Xiaoli , Ai Zexin , Yu Miao , Wu Yang’ou , LI SHENGJIAO 
Published: March 2020

Abstract
Background There is a growing body of evidence suggesting that long non-coding RNAs(lncRNAs) can function as a microRNA(miRNA) sponge in various diseases including oral cancer. However, we are still not very clear about the pathophysiological function of lncRNAs. Methods We constructed a lncRNA-miRNA-mRNA network in oral cancer based on the competitive endogenous RNA(ceRNA) theory with the human expression profiles GSE74530 from the Gene Expression Omnibus (GEO) database and used topological analysis to determine the hub lncRNAs in the regulatory ceRNA network. Then, function enrichment analysis was performed by R package Cluster Profiler. Results A total of 238 potential co-dysregulated competing triples were obtained in the lncRNA-assoicated ceRNA network of oral cancer, which consisted of 10 lncRNA nodes, 41 miRNA nodes, and 122 mRNA nodes. Additionally, we found three lncRNAs( HCP5 , AGAP11 , HCG22 ) exhibiting superior potential as diagnostic and prognostic markers of oral cancer. Conclusions Our findings will provide novel insights for understanding the ceRNA regulation in oral cancer and identify three novel lncRNAs as potential molecular biomarkers.
Comprehensive analyses to identify LINC02310 as an enhancer in lung adenocarcinoma
Authors: Zhao Wenyuan , Wang Jun , Luo Qingxi , Peng Wei , Li Bin , Wang Lei , Zhang Chunfang , Duan Chaojun 
Published: March 2020

Abstract
Lung adenocarcinoma (LADC) is a major subtype of non-small cell lung cancer (NSCLC) and has one of the highest mortality rates. An increasing number of long non-coding RNAs (lncRNAs) were reported to be associated with the occurrence and progression of LADC. In order to find potential biomarkers for LADC therapies among lncRNAs, 2431 differentially expressed lncRNAs (DElncRNAs) over LADC and adjacent normal samples were identified from The Cancer Genome Atlas (TCGA) expression data. Lnc-YARS2-5, lnc-NPR3-2 and LINC02310, which were the top-3 significant DElncRNAs related to overall survival, were selected for further analysis. Their overexpression indicated poor prognostic. PCR experimental results also showed that they were mainly up-regulated in the LADC tissues from Xiangya Hospital. Clinical analysis of these cases suggested that LINC02310 was significantly correlated with TNM-stage and T-stage, which was further validated by the clinical analysis based on TCGA. Reasonably, we assumed that LINC02310 acted as an enhancer in LADC, which was demonstrated by CCK-8 assay and colony formation assay. Moreover, 3 targeted miRNAs of LINC02310 and 414 downstream differentially expressed mRNAs (DEmRNAs) were predicted. The competing endogenous RNA (ceRNA) regulatory network was constructed with LINC02310, 3 miRNAs and 113 DEmRNAs involved. The downstream mRNAs were then enriched in 405 GO terms and 11 KEGG pathways, which revealed their potential functions and mechanisms. The protein-protein interaction (PPI) network showed the relationships between the downstream mRNAs. In conclusion, this study verified LINC02310 as an enhancer in LADC and conducted comprehensive analyses on its target miRNAs and downstream mRNAs.
Up-regulation of miR-1825 inhibits the progression of glioblastoma by suppressing CDK14 though Wnt/β-catenin signaling pathway
Authors: Lu Fengqin , Li Chunhong , Sun Yuping , Jia Ting , Li Na , Li Haiyan 
Published: March 2020

Abstract
Mounting evidences displayed that miRNAs modulated glioblastoma (GBM) development. Here, our goal was to investigate whether miR-1825 could regulate GBM development and explore its potential mechanism. Our findings displayed that miR-1825 was decreased in GBM tissue specimens by qRT-PCR and it confirmed as a prognostic marker of GBM by Kaplan-Meier survival analysis. Moreover, we also found that miR-1825 up-regulation suppressed GBM cell viability, tumor growth, invasion and migration by MTT assay, Xenograft assay and Transwell assay. Furthermore, CDK14 was first identified as the target of miR-1825 by Luciferase reporter assay and CDK14 acted as an oncogene in GBM development by Immunohistochemistry. In addition, Western blot analysis demonstrated that Wnt/β-catenin signaling pathway took part in GBM development modulating by miR-1825. In conclusion, miR-1825 up-regulation suppressed GBM progression by targeting CDK14 through Wnt/β-catenin pathway.
Internet-based cognitive rehabilitation for WORking Cancer survivors (i-WORC): study protocol of a randomized controlled trial
Authors: Klaver Kete , Duijts Saskia , Geusgens Chantal , Aarts Maureen , Ponds Rudolf , Beek Allard van der , Schagen Sanne 
Published: March 2020

Abstract
Background Cognitive problems are common in non-central nervous system cancer survivors. These problems are perceived as an important contributor to decline in work performance and work ability. Various interventions for cognitive problems have been proposed, but effectiveness regarding work-related outcomes has not yet been established. Effective treatment options to alleviate the adverse influence of cognitive problems on work performance are needed for working cancer survivors. In this paper, we will describe the design of a randomized, controlled, multicenter trial that evaluates the (cost-)effectiveness of an internet-based cognitive rehabilitation program for occupationally active cancer survivors confronted with cognitive problems. Methods/ Design A three-armed randomized controlled trial will be conducted, including two intervention groups (i.e., basic and extensive cognitive rehabilitation program) and one waitlist control group. In total, 261 cancer survivors (18 – 65 years) who have returned to work, and who experience cognitive problems will be recruited. Patients with and without cognitive impairment as established in a neuropsychological assessment, will be eligible; stratification will take place based on the presence of this cognitive impairment. The extensive intervention arm will contain a comprehensive training program (including psycho-education, fatigue management, and cognitive strategy training) with individual guidance (blended intervention). The basic intervention arm will contain a brief cognitive training program (including psycho-education and fatigue management) without individual guidance. The primary outcome will be accomplishment of an individually defined work-related treatment goal. Secondary outcomes include, among others, subjective cognitive functioning, work functioning, and quality of life. Primary and secondary outcomes will be measured at baseline (T0), and at 12 weeks (T1) and 26 weeks (T2) post-randomization. Discussion About 40-50% of the cancer patients worldwide are of working age at time of diagnosis. Many of the occupationally active cancer survivors experience cognitive problems. Both from an individual and a societal perspective, it is important to sustain cancer survivors’ employability. An effective treatment to alleviate the impact of cognitive decline and to improve work ability might help cancer survivors to sustain employability. Trial registration The trial is registered at ClinicalTrials.gov (NCT03900806) at 03 April 2019 (current status: ongoing),https://clinicaltrials.gov/ct2/show/NCT03900806?term=NCT03900806&draw=2&rank=1. KEYWORDS Cancer-related cognitive impairment, Cognitive rehabilitation, Employment, Internet-based, Randomized controlled trial, Cost-effectiveness
Examining Long-Term Effects of Human Papillomavirus Vaccine Recommendation Messages: A 4-months Follow-up Survey of a Randomized Controlled Study in Japan
Authors: Okuhara Tsuyoshi , Ishikawa Hirono , Ueno Haruka , Okada Hiroko , Kiuchi Takahiro 
Published: March 2020

Abstract
Background: We previously conducted a randomized controlled study to examine persuasive messages recommending HPV vaccination to mothers with daughters in September 2017 in Japan. Our previous study showed that the three types of intervention message used (statistical information only, a patient’s narrative in addition to statistical information, and a mother’s narrative in addition to statistical information) all significantly improved mothers’ intention to have their daughter(s) receive the HPV vaccine, in comparison with mothers who received no messaging. The present study is a follow-up survey to assess the long-term effect of the intervention in our previous study. Methods: Four months after the initial study, in January 2018, participants in the previous study were contacted and queried about their current intention to have their daughter(s) receive the HPV vaccine. Statistical analysis was conducted using the paired t -test and analysis of variance. Results: A total of 978 mothers participated in the current survey. Vaccination intention 4 months after intervention had decreased to a level that did not differ significantly from the level prior to intervention in all three intervention conditions. The amount of change in vaccination intention 4 months after intervention did not differ significantly among the three intervention groups ( p =0.871). Conclusions: A single exposure to messaging was insufficient to produce a persistent intervention effect; repeated exposure to messaging coupled with other interventions may be important.
Lung ultrasound in patients with rheumatoid arthritis and the definition of significant interstitial lung disease
Authors: Carlo Marco Di , Tardella Marika , Filippucci Emilio , Carotti Marina , Salaffi Fausto 
Published: March 2020

Abstract
Background

In recent years, a growing interest has grown around interstitial lung disease (ILD) in patients with rheumatoid arthritis (RA). While high resolution computed tomography (HRCT) of the chest remains the diagnostic method of choice, increasing attention has been directed towards lung ultrasound (LUS) in the diagnosis of ILD in connective tissue diseases. However, it is not yet well defined how to interpret the LUS findings under suspicion of RA-ILD. The aim of this study was to determine the cut-off number of LUS B-lines that identifies a significant RA-ILD.

Methods

A cross sectional study was conducted on consecutive RA patients with suspected RA-ILD. The inclusion criteria were clinical (dyspnea, velcro sounds), instrumental (suggestive anomalies on conventional radiography, DLco reduction), or in presence of at least two of the following risk factors for RA-ILD: smoking habit, male sex, advanced age, and ACPA presence.
Patients underwent LUS (carried out in 14 defined intercostal spaces), chest HRCT, pulmonary function tests, and clinical evaluation. The diagnosis of RA-ILD was based on a semi-quantitative evaluation of chest HRCT using a computer-aided method (CaM). The discriminative validity of the LUS versus HRCT has been studied by using the receiver operating characteristic (ROC) curve analysis.

Results

72 consecutive RA patients (21 male, 51 female) were evaluated, with a mean age of 63.0 (SD 11.5 years). The mean estimate of pulmonary fibrosis using the CaM was 11.20% (SD 7.48) at chest HRCT, while at LUS the mean number of B-lines was 10.65 (SD 15.11). A significant RA-ILD, as measured by the CaM at HRCT, was detected in 25 patients (34.7%). The presence of 9 B-lines was found to be the optimal cut-off at ROC curve analysis. This LUS cut-off defines the presence of significant RA-ILD with a sensitivity of 70.0%, a specificity of 97.62%, and a positive likelihood ratio of 29.4.

Conclusion

The present study provided data to determine the number of B-lines to identify a significant RA-ILD. LUS may represent a useful technique to select RA patients to be assessed by chest HRCT.
MicroRNA-559 restrains gastric cancer progression via activating AKT signaling pathway by targeting TRIM14
Authors: Wei Li , Zhang Lu , Zhang Bo , Wang Guanghui , Meng Lei , Zhang Junbo , Meng Yan , Qin Hao 
Published: March 2020

Abstract
Background: MicroRNAs (miRNAs) act as pivotal functions in gastric cancer (GC) carcinogenesis and progression. MiR-559 has been defined as a potential cancer suppressor gene in a few cancers. Nevertheless, the biological effect of miR-559 in human GC and the underlying molecular mechanism still unclear and need to be further illuminated. Methods: Quantitative real-time PCR (qRT-PCR) was fulfilled for measuring the miR-559 expression level in GC. The dual-luciferase reporter was used to verify that tripartite motif-containing 14 (TRIM14) is a target gene of miR-559. The expression levels of TRIM14 were examined by qRT-PCR and Western blot in GC tissue specimens and cell lines. The effects of miR-559 on GC cell growth were detected with MTT and cell counting assays. Cell cycle and apoptosis were examined by using flow cytometry. Overexpression and siRNA further demonstrated the role of TRIM14 in GC. Results: Our results revealed that miR-559 expression was dramatically downregulated in human GC tissue specimens and cell lines. MiR-559 overexpression restrained GC cell growth, and induced cell cycle G1-S phase arrest and apoptosis. MiR-559 inhibitors promoted cell multiplication and cell cycle G1-S transition, and inhibited apoptosis. MiR-559 level was negatively related to TRIM14 mRNA level in GC. The results showed that TRIM14 was affirmed to be a target gene of miR-559. MiR-559 overexpression downregulated the TRIM14 expression, and miR-559 inhibitors upregulated the TRIM14 expression. Particularly, knockdown of TRIM14 gave rise to the semblable cellular effects observed upon miR-559 overexpression. TRIM14 overexpression recapitulated the revulsive cellular and molecular effects by suppression of miR-559. Furthermore, both miR-559 overexpression and TRIM14 silencing led to the inhibition of the AKT signaling pathway. On the contrary, miR-559 suppression and TRIM14 overexpression activated the AKT signaling pathway, and inhibited Bax/Bcl-2 pathway. Conclusions: The findings illustrate that miR-559 restrains cell multiplication via suppressing the AKT signaling pathway in human GC and induces cell apoptosis through the suppression of Bax/Bcl-2 signaling pathway via targeting TRIM14. The findings imply that miR-559 acts as a pivotal function in human GC and represents a latent novel target in GC therapy.
The value of circulating tumor cells with positive centromere probe 8 in the diagnosis of small pulmonary nodules.
Authors: Liu Caidong , Chen Hongling , Sun Tong , Wang Haibo , Chen Baoan , Wang Xuerong 
Published: March 2020

Abstract
Background Circulating cancer cells (CTCs) provide opportunities for early diagnosis and evaluation of cancer stage as a more acceptable non-invasive liquid biopsy. The advanced development and use of CTCs for diagnosis or prognosis just started in recent years. Methods Fifty three patients, diagnosed as SPNs with a diameter less than 30 mm by CT examination, was enrolled for statistical analysis based on their CTCs level, CT examination features, serum tumor marker concentrations, and histopathological characteristics. Centromere probe 8 (CEP8) was utilized as a marker for CTCs determination. Results The CTCs level was significantly different between malignant and benign SPNs, as well as between early (0/Ia) and advanced (Ib/II/III) stages of lung cancer. For the malignancy judgment and the pTNM stage diagnosis of SPNs, ROC analysis results showed that combined use of CTCs level or density feature of CT morphology significant improved diagnostic effect compared to use of these two markers solely. Moreover, in bigger, single, and solid SPNs based on CT morphology, the CTCs level significantly correlated with the malignant histopathology. Additionally, triple staining (CEP8, EpCAM and CKs) results using samples from 22 out of 53 patients showed that more CTCs was detected when CEP8 was used as a marker. Conclusion The CTCs determined by CEP8 positive would be a potential adjuvant diagnostic marker for the malignance and stage of lung cancer for patients with SPNs.
PD1 and PD-L1 Inhibitors for the Treatment of Kidney Cancer: The Role of PD-L1 Assay
Authors: Cimadamore Alessia , Massari Francesco , Santoni Matteo , Lopez-Beltran Antonio , Cheng Liang , Scarpelli Marina , Montironi Rodolfo , Moch Holger 
Published: March 2020

Background:
Immune checkpoint inhibitors targeting the programmed death receptor ligand
1 (PD-L1)/programmed death receptor 1 (PD-1) pathway represent a drastic change in the treatment
landscape of RCC resulting in a dynamic and evolving scenario. There is an urgent need for predictive
biomarkers of response to provide a personalized therapeutic strategy for individual patients.

Objective:
In this review, we focused on trials that investigated the administration of a PD-1 and PDL1
inhibitor alone or in combination with another agent and compared the different assays applied in
each trial to evaluate the role of PD-L1 as a prognostic and predictive biomarker.

Conclusion:
So far, the use of PD-L1 expression alone is not sufficient to predict treatment response
and present many limitations: the lack of consensus between different methodologies on biomarker
assessment, the heterogeneity of PD-L1 between primary tumors and metastatic sites, different criteria
of response to therapy (RECIST vs. irRECIST), the complex interplay with inflammatory components,
previous treatments, administration of antibiotic therapy. Combinations of different biomarkers and
biological features, such as gene expression associated with angiogenesis, immune response and myeloid
inflammation are promising biological variables that need to be validated in the context of prospective
clinical trials.