bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA BC088259 promotes Schwann cell migration through Vimentin following peripheral nerve injury
Authors: Yao Chun , Chen Yanping , Wang Jing , Qian Tianmei , Feng Wei , Chen Yuanyuan , Mao Susu , Yu Bin 
Published: November 2019

AbstractSchwann cell, the major glial cell in the peripheral nervous system, plays an essential role in peripheral nerve regeneration. However, the regulation of Schwann cell behavior following nerve injury is insufficiently explored. According to the development of high‐throughput techniques, long noncoding RNAs (lncRNAs) have been recognized. Accumulating evidence shows that lncRNAs take part in diverse biological processes and diseases. Here, by microarray analysis, we identified an upregulated lncRNA profile following sciatic nerve injury and focused on BC088259 for further investigation. Silencing or overexpression of BC088259 could affect Schwann cell migration. Mechanistically, BC088259 might exert this regulatory role by directly binding with Vimentin. Collectively, our study not only revealed a set of upregulated lncRNAs following nerve injury but also identified a new functional lncRNA, BC088259, which was important for Schwann cell migration, providing a therapeutic avenue toward peripheral nerve injury.
Predictors of online accountability practices in US hospitals: An exploratory investigation
Authors: Cooley Asya 
Published: November 2019

SummaryThe purpose of this research paper is to explore variations in online accountability practices in US hospitals and determine the factors that are associated with higher levels of online accountability practices. This project employed a quantitative content analysis of 240 US hospital websites. Additionally, secondary data were obtained from the American Hospital Association and the American Hospital Directory. The results show that the external environment somewhat impacted hospitals' online accountability practices, with hospital volume (measured through the number of annual admissions) as an unquestionable predictor. Another key finding is that some of the governance forms impacted online accountability practices. Particularly, hospitals with private ownership structures tended to disclose less accountability information in an online environment, compared with their public and nonprofit counterparts. The financial situation of hospitals did not have any significant impact on overall online accountability practices but was influencing performance disclosure practices. Online accountability studies have not been conducted in a health care setting. This research theoretically relates online accountability practices to organizational characteristics (such as size, volume, financial performance, system affiliation, ownership, and rurality). Knowledge of the online accountability landscape might benefit future policy decisions on accountability models.
Non‐performing loans and the harmonisation of extrajudicial collateral enforcement across Europe
Authors: Schuijling Ben , van Hoof Vincent , Hutten Tom 
Published: November 2019

AbstractThe European Union plans on partially harmonising the extrajudicial enforcement of collateral in order to reduce the stocks of nonperforming loans. This article discusses the proposed regime and its background. After examining its impact on the national secured transactions law of Austria, Belgium, France, Germany and The Netherlands, the authors comment on the appropriateness of the proposed harmonisation.
Optimizing deep belief network parameters using grasshopper algorithm for liver disease classification
Authors: Renukadevi Thangavel , Karunakaran Saminathan 
Published: November 2019

AbstractImage processing plays a vital role in many areas such as healthcare, military, scientific and business due to its wide variety of advantages and applications. Detection of computed tomography (CT) liver disease is one of the difficult tasks in the medical field. Hand crafted features and classifications are the two types of methods used in the previous approaches, to classify liver disease. But these classification results are not optimal. In this article, we propose a novel method utilizing deep belief network (DBN) with grasshopper optimization algorithm (GOA) for liver disease classification. Initially, the image quality is enhanced by preprocessing techniques and then features like texture, color and shape are extracted. The extracted features are reduced by utilizing the dimensionality reduction method like principal component analysis (PCA). Here, the DBN parameters are optimized using GOA for recognizing liver disease. The experiments are performed on the real time and open source CT image datasets which embraces normal, cyst, hepatoma, and cavernous hemangiomas, fatty liver, metastasis, cirrhosis, and tumor samples. The proposed method yields 98% accuracy, 95.82% sensitivity, 97.52% specificity, 98.53% precision, and 96.8% F‐1 score in simulation process when compared with other existing techniques.
Step‐by‐step monitoring of a magnetic and SERS‐active immunosensor assembly for purification and detection of tau protein
Authors: Maurer Viktor , Frank Claudia , Porsiel Julian Cedric , Zellmer Sabrina , Garnweitner Georg , Stosch Rainer 
Published: November 2019

AbstractWe report a bottom‐up synthesis of iron oxide and gold nanoparticles, which are functionalized and combined to form a nanohybrid serving as an immune sensor, which selectively binds to tau protein, a biomarker for diagnosis of Alzheimer's disease. Detection of the target analyte is achieved by surface‐enhanced Raman scattering originating from the diagnostic part of the nanohybrid that was prepared from Au nanoparticles functionalized with 5,5′‐dithiobis‐(2‐nitrobenzoic acid) as a Raman reporter and monoclonal anti‐tau antibody. The magnetic part consists of FexOy nanoparticles functionalized with polyclonal anti‐tau antibody and is capable to separate tau protein from a complex matrix such as cerebrospinal fluid. We further identified and validated a set of analytical tools that allow monitoring the success of both nanoparticle preparation and each functionalization step performed during the assembly of the two binding sites by an immune reaction. By applying UV/Vis spectroscopy, dynamic light scattering, zeta potential measurements, X‐ray diffraction, small‐angle X‐ray scattering, and transmission electron microscopy, we demonstrate a proof‐of‐concept for a controlled and step‐by‐step traceable synthesis of a tau protein‐specific immune sensor.
LncRNA TDRG1 promotes the metastasis of NSCLC cell through regulating miR‐873‐5p/ZEB1 axis
Authors: Hu Xiufeng , Mu Yu , Wang Jiaojie , Zhao Yanqiu 
Published: November 2019

AbstractLong noncoding RNAs (lncRNAs) are dysregulated in various malignancies and involved in the growth and aggressive phenotypes of cancer cells. Previous studies indicate that lncRNA testis development related 1 (TDRG1) plays critical roles in the development of several malignancies. Nevertheless, the molecular mechanism underlying TDRG1 contributes to non–small cell lung cancer (NSCLC) remains elusive. Here, we demonstrate that TDRG1 is significantly overregulated in NSCLC tissues and cell lines. Knockdown of TDRG1 inhibits the proliferation and metastatic‐related traits of NSCLC cell in vitro whereas overexpression of TDRG1 causes opposite results. In addition, TDRG1 silencing inhibits the growth and metastatic ability of NSCLC cell in vivo as demonstrated by xenograft tumor model and lung metastasis model. The binding capacity of TDRG1 with miR‐873‐5p is demonstrated by bioinformatics prediction tool and luciferase reporter gene assay. Additional, the rescue experiments indicate that TDRG1 interacts with miR‐873‐5p and its expression is positively associated with the target of miR‐873‐5p, zinc finger e‐box binding homeobox 1 (ZEB1). Altogether, lncRNA TDRG1 facilitates the progression of NSCLC via interacting with miR‐873‐5p and positively regulates the expression of ZEB1.
Long noncoding RNA NEAT1 correlates with higher disease risk, worse disease condition, decreased miR‐124 and miR‐125a and predicts poor recurrence‐free survival of acute ischemic stroke
Authors: Li Ping , Duan Shuyuan , Fu Adan 
Published: November 2019

AbstractObjectiveThis study aimed to investigate the predictive value of long noncoding RNA nuclear enriched abundant transcript 1 (lncRNA NEAT1) for acute ischemic stroke (AIS) risk and to explore the correlation of lncRNA NEAT1 with disease severity, inflammation, recurrence and target microRNAs in patients with AIS.Methods210 patients with AIS and 210 controls were enrolled, and their peripheral blood samples were collected within 24 hours after admission and collected on the enrollment, respectively. lncRNA NEAT1 expression was detected by quantitative polymerase chain reaction (qPCR). For patients with AIS, disease severity was evaluated by National Institute of Health Stroke Scale (NIHSS) score; plasma concentrations of inflammatory factors and lncRNA NEAT1 target microRNAs were measured by enzyme‐linked immune sorbent assay and qPCR, respectively; stroke recurrence and death were recorded; and recurrence‐free survival (RFS) was calculated.ResultslncRNA NEAT1 expression was elevated in patients with AIS compared with controls, and it had a good predictive value for AIS risk (area under the curve [AUC]: 0.804 [95% confidence interval [CI]: 0.763‐0.845]). In patients with AIS, lncRNA NEAT1 expression positively correlated with NIHSS score and inflammatory factor levels including C‐reactive protein (CRP), tumor necrosis factor (TNF)‐α, interleukin (IL)‐6, IL‐8, and IL‐22, while it negatively correlated with anti‐inflammatory cytokine IL‐10 level. Besides, lncRNA NEAT1 predicted increased recurrence/death risk (AUC: 0.641 [95% CI: 0.541‐0.741]), and its high expression correlated with worse RFS. Additionally, lncRNA NEAT1 expression negatively correlated with microRNA‐124 and microRNA‐125a expressions.ConclusionLncRNA NEAT1 may serve as a novel biomarker for assisting AIS management and prognosis.
Reduced expression of circRNA hsa_circ_0067582 in human gastric cancer and its potential diagnostic values
Authors: Yu Xiuchong , Ding Haixiang , Yang Liangwei , Yu Yu , Zhou Jiaming , Yan Zhilong , Guo Junming 
Published: November 2019

AbstractBackgroundGastric cancer (GC) is one of the global mortality diseases and has a poor prognosis due to the lack of ideal tumor biomarkers. Circular RNAs (circRNAs) are an abundant kind of endogenous RNAs that recently are found play a crucial role in the cancer occurrence and development. Nevertheless, little is known with regard to the diagnostic values of these circRNAs for GC. In this article of research, we investigated the role of hsa_circ_0067582 in clinical diagnosis of GC.Materials and MethodsWe used divergent primers, and the expression levels of hsa_circ_0067582 in 93 fresh GC tissues and paired adjacent normal tissues from surgical patients were detected using quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). Then, a receiver operating characteristic (ROC) curve was established to assess the diagnostic significance of hsa_circ_0067582. The relationship between expression of hsa_circ_0067582 and clinicopathological factors of patients was made further explored.ResultsHsa_circ_0067582 levels were significantly decreased in GC tissues contrasted with adjacent normal tissues (n = 93, P < .001). After that, we discovered that it was evidently downregulated in 81.7% (76/93) GC tissues. The area under the ROC curve (AUC) of hsa_circ_0067582 was up to 0.6937, the sensitivity was 66.67%, and the specificity was 61.29%. Moreover, the hsa_circ_0067582 levels were obviously associated with tumor diameter (P = .002) and carbohydrate antigen 19‐9 (CA19‐9, P = .01). Meanwhile, after operation, low‐level group of hsa_circ_0067582 of GC patients was associated with better prognosis.ConclusionOur data imply that hsa_circ_000067582 may be a potential biomarker for GC diagnosis and prognosis evaluation.
Modular cognitive‐behavioral therapy for affective symptoms in young individuals at ultra‐high risk of first episode of psychosis: Randomized controlled trial
Authors: Pozza Andrea , Dèttore Davide 
Published: November 2019

AbstractObjectives: Individuals at ultra‐high risk of psychosis often present concurrent affective symptoms (depression/anxiety). This study investigated whether modular cognitive‐behavioral therapy (CBT) targeting both ultra‐high risk and affective symptoms (a) reduced/delayed risk of a first psychotic episode at posttreatment and 14‐month follow‐up compared with a supportive intervention, (b) was more effective than control condition in producing remission on depression/anxiety.Methods: Fifty‐eight ultrahigh risk individuals were randomly assigned to CBT or control condition. CBT consisted of 30 sessions, including CBT for psychotic experiences and depression/anxiety.Results: In the CBT group, the cumulative number of participants who developed a first psychotic episode (n = 3, 10.34%) at follow‐up was lower than in the control group (n = 8, 27.60%; logrank χ2(1) = 3.68, p = .05). In the CBT group, a higher number of participants achieved remission than in control condition on affective symptoms at posttreatment/follow‐up.Conclusion: CBT can prevent psychosis risk and produce better outcomes on depression/anxiety than supportive intervention.
Hepatic hemangioma supplied by abnormal portal vein: A case report
Authors: Ogawa Masahiro , Matsumoto Naoki , Kaneko Masahiro , Kumagawa Mariko , Watanabe Yukinobu , Hirayama Midori , Moriyama Mitsuhiko , Higaki Tokio , Takayama Tadatoshi , Sugitani Masahiko 
Published: November 2019

AbstractHepatic hemangioma is the most common hepatic tumor with a prevalence of approximately 3%. It is typically supplied by the hepatic artery as evident from findings of abdominal angiography, contrast‐enhanced ultrasonography (CEUS), contrast‐enhanced computed tomography (CT), and contrast‐enhanced magnetic resonance imaging. However, few cases of hepatic hemangioma supplied by the portal vein have been reported. In this paper, we report a rare case of hepatic hemangioma supplied by the portal vein as shown on CEUS and CT arterioportography.
Exome sequencing identifies a FHOD3 p.S527del mutation in a Chinese family with hypertrophic cardiomyopathy
Authors: Huang Suqiu , Pu Tian , Wei Wei , Xu Rang , Wu Yurong 
Published: November 2019

AbstractBackgroundHypertrophic cardiomyopathy (HCM) is the most common inheritable cardiac disease and is characterised by unexplained ventricular myocardial hypertrophy. HCM is highly heterogeneous and is primarily caused by the mutation of genes encoding sarcomere proteins. As a result of its genetic basis, we investigated the underlying cause of HCM in a Chinese family by whole‐exome sequencing.MethodsWhole‐exome sequencing was performed for seven clinically diagnosed HCM family members and the resulting single nucleotide variants associated with cardiac hypertrophy or heart development were analysed by a polymerase chain reaction and Sanger sequencing.ResultsA non‐frameshift deletion mutation (p.S527del) of Formin Homology 2 Domain Containing 3 (FHOD3) was detected in all of the affected family members and was absent in all unaffected members, with the exception of one young member. Moreover, three single nucleotide variants associated with heart development and morphogenesis were identified in the proband but were absent in the other affected subjects.ConclusionsThis is the first HCM family case of FHOD3 (p.S527del) variation in Asia. Additionally, RNF207 (p.Q268P), CCM2 (p. E233K) and SGCZ (p.Q134X) may be related to the clinical heterogeneity of the family. The present study could enable the provision of genetic counseling for this family and provide a basis for future genetic and functional studies.
Crosstalk between myeloid-derived suppressor cells and the immune system in prostate cancer
Authors: Sanaei Mohammad-Javad , Salimzadeh Loghman , Bagheri Nader 
Published: November 2019

AbstractProstate cancer is the second most common cancer and the fifth leading cause of cancer-associated death in men. Previous studies have revealed a surprising ability for an immature population of myeloid cells called myeloid-derived suppressor cells (MDSCs) in the commencement and development of many tumors, including those of prostate cancer. Herein, the molecular and cellular changes of MDSCs in prostate cancer in both human and nonhuman models are reviewed. The suppressive function of MDSCs are also discussed with a particular focus on the role of IL-6 and JAK/STAT3 signaling pathways in the induction of their suppressive activity. Ultimately, a brief review of MDSC-targeting approaches for potential cancer therapy is presented.
Sequential CCL2 Expression Profile After Disc Injury in Mice
Authors: Nakawaki Mitsufumi , Uchida Kentaro , Miyagi Masayuki , Inoue Gen , Kawakubo Ayumu , Kuroda Akiyoshi , Satoh Masashi , Takaso Masashi 
Published: November 2019

ABSTRACTMacrophages produce proinflammatory cytokines in injured intervertebral discs (IVDs). We recently showed that macrophage‐derived inflammatory cytokines contribute to the production of pain‐related factors. However, the mechanism by which macrophages are recruited to injured IVDs has not been fully clarified. Here, we examined the expression dynamics of the chemokine CCL2 in a mouse IVD injury model and the mechanisms of its regulation. The percentage of macrophages increased from day 1 after injury and persisted up until day 28. At 1 and 3 days after injury, the expression of both Ccl2 messenger RNA (mRNA) and CCL2 protein was elevated in the IVD injury group, after which expression decreased to basal levels. Consistent with the increase in CCL2 expression, Ccr2 and Tnfa expression and various types of macrophages were also immediately elevated following disc injury. Further, tumor necrosis factor‐α (TNF‐α) stimulated Ccl2 mRNA and CCL2 protein expression in IVD cells in vitro. The expressions of M1 (Cd86 and Nos2) and M2a (Ym1) macrophage markers were all significantly elevated from  day 1 following injury in injured compared with control mice. Meanwhile, the expression of Cd206 (M2a and M2c marker) was significantly elevated on days 3, 7, 14, and 28 following injury. These results suggest that in IVD injury, TNF‐α stimulates CCL2, which, in turn, mediates the recruitment of macrophages with the recruited macrophages subsequently differentiating into M1 and M2 subtypes. CCL2 signaling may, therefore, play an important role in IVD pathology via macrophage recruitment. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:895‐901, 2020
Cellular Plasticity in Musculoskeletal Development, Regeneration, and Disease
Authors: Kaji Deepak A. , Tan Zhijia , Johnson Gemma L. , Huang Wesley , Vasquez Kaetlin , Lehoczky Jessica A. , Levi Benjamin , Cheah Kathryn S. E. , Huang Alice H. 
Published: November 2019

ABSTRACTIn this review, we highlight themes from a recent workshop focused on “Plasticity of Cell Fate in Musculoskeletal Tissues” held at the Orthopaedic Research Society's 2019 annual meeting. Experts in the field provided examples of mesenchymal cell plasticity during normal musculoskeletal development, regeneration, and disease. A thorough understanding of the biology underpinning mesenchymal cell plasticity may offer a roadmap for promoting regeneration while attenuating pathologic differentiation. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:708‐718, 2020
Shedding light into the health‐beneficial properties of Corema album—A vibrational spectroscopy study
Authors: Martin D. , Marques J. , Amado A.M. , Barroca M.J. , Moreira da Silva A. , Batista de Carvalho L.A.E. , Marques M.P.M. 
Published: November 2019

AbstractCorema album (L.) D. Don is a wild maritime shrub endemic to the Iberian Peninsula, which contains bioactive compounds with promising chemoprotective activity. The present work reports the first study of the edible fruits of this potentially health‐beneficial plant by complementary Raman and infrared techniques. Unique vibrational signatures were obtained for each part of the Corema album berries, revealing distinct chemical compositions for the skin (outer and inner) and the seeds, particularly regarding the content in phenolic derivatives, unsaturated fatty acids, and waxy polymers.
Photodynamic therapy may provide a benefit over systemic chemotherapy among non‐surgically managed patients with extrahepatic cholangiocarcinoma
Authors: Wu Lu , Merath Katiuscha , Farooq Ayesha , Hyer J. Madison , Tsilimigras Diamantis I , Paredes Anghela Z. , Mehta Rittal , Sahara Kota , Shen Feng , Pawlik Timothy M. 
Published: November 2019

AbstractBackgroundSystemic chemotherapy is the standard treatment for patients with unresectable extrahepatic cholangiocarcinoma (ECC), however, the survival benefit of chemotherapy is limited. Photodynamic therapy (PDT) has been associated with improved survival among patients with advanced ECC, yet utilization of PDT remains low. We sought to compare the outcomes of patients with unresectable ECC following treatment with PDT versus chemotherapy.MethodsA review of the National Cancer Database was conducted to identify patients with ECC who were nonsurgically managed between 2004 and 2013. Overall survival (OS) of patients receiving PDT vs systemic chemotherapy was compared using propensity score matching.ResultsAfter propensity matching (PDT, n = 59; chemotherapy, n = 177), 5‐year OS was 17.6% (95% confidence interval [CI], 9.0%‐28.6%) among patients who underwent PDT vs 3.8% (95%CI, 0.4%‐14.0%) among patients receiving chemotherapy (P < .001). On multivariable analysis PDT was associated with an OS benefit (hazard ratio, 0.72; 95%CI, 0.52‐0.998; P = .048). Subset analysis of patients receiving PDT only (n = 45) and patients receiving chemotherapy demonstrated similar results. In subset analysis of patients undergoing PDT‐only vs PDT‐chemotherapy, OS was comparable.ConclusionPDT was associated with a survival benefit compared with chemotherapy alone among patients with unresectable ECC.
The prognostic value of anterior commissure involvement in T1 glottic cancer: A systematic review and meta‐analysis
Authors: Tulli Michele , Re Massimo , Bondi Stefano , Ferrante Luigi , Dajko Marianxhela , Giordano Leone , Gioacchini Federico M. , Galli Andrea , Bussi Mario 
Published: November 2019

ObjectiveThe anterior commissure is considered a laryngeal subsite, but it is not taken into account in the current T‐staging system. The impact of anterior commissure involvement (ACI) on the oncologic outcomes of T1 glottic tumors has not been well defined and remains controversial. The aim of this study was to assess the influence of ACI in the prognosis of T1 glottic tumors.MethodsA systematic review and meta‐analysis of observational studies was performed by including studies published up to July 2019. Studies were selected if they had investigated the local control rate (LCR) at 5 years of clinical stage I (T1 and N0) glottic squamous cell carcinoma with and without ACI. The difference in LCR at 5 years between T1 glottic tumors without and with ACI was evaluated.ResultsThe meta‐analysis showed that the difference in LCR at 5 years between T1 glottic tumors without and with ACI is 12% (95% confidence interval: 8%‐16%, p < 0,0001, I2 = 34.81%).ConclusionsOur study pointed out that the anterior commissure involvement is a negative prognostic factor in LCR at 5 years for T1 glottic tumors. Our results suggest the need to consider ACI in the T staging of glottic tumors. Laryngoscope, 130:1932–1940, 2020