bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Great Acceleration of fragrances and PAHs archived in an ice core from Elbrus, Caucasus
Authors: Vecchiato Marco , Gambaro Andrea , Kehrwald Natalie M. , Ginot Patrick , Kutuzov Stanislav , Mikhalenko Vladimir , Barbante Carlo 
Published: June 2020

AbstractThe Great Acceleration of the anthropogenic impact on the Earth system is marked by the ubiquitous distribution of anthropogenic materials throughout the global environment, including technofossils, radionuclides and the exponential increases of methane and carbon dioxide concentrations. However, personal care products as direct tracers of human domestic habits are often overlooked. Here, we present the first research combining fragrances, as novel personal care products, and polycyclic aromatic hydrocarbons (PAHs) as combustion and industrial markers, across the onset of the Great Acceleration in the Elbrus, Caucasus, ice core. This archive extends from the 1930s to 2005, spanning the profound changes in the relationship between humans and the environment during the twentieth century. Concentrations of both fragrances and PAHs rose throughout the considered period, reflecting the development of the Anthropocene. However, within this rising trend, remarkable decreases of the tracers track the major socioeconomic crises that occurred in Eastern Europe during the second half of the twentieth century.
Combined and independent effects of hypoxia and tributyltin on mRNA expression and physiology of the Eastern oyster (Crassostrea virginica)
Authors: Barnett Ann Fairly , Gledhill James H. , Griffitt Robert J. , Slattery Marc , Gochfeld Deborah J. , Willett Kristine L. 
Published: June 2020

AbstractOyster reefs are vital to estuarine health, but they experience multiple stressors and globally declining populations. This study examined effects of hypoxia and tributyltin (TBT) on adult Eastern oysters (Crassostrea virginica) exposed either in the laboratory or the field following a natural hypoxic event. In the laboratory, oysters were exposed to either hypoxia followed by a recovery period, or to hypoxia combined with TBT. mRNA expression of HIF1-α and Tβ-4 along with hemocyte counts, biomarkers of hypoxic stress and immune health, respectively, were measured. In field-deployed oysters, HIF1-α and Tβ-4 expression increased, while no effect on hemocytes was observed. In contrast, after 6 and 8 days of laboratory-based hypoxia exposure, both Tβ-4 expression and hemocyte counts declined. After 8 days of exposure to hypoxia + TBT, oysters substantially up-regulated HIF1-α and down-regulated Tβ-4, although hemocyte counts were unaffected. Results suggest that hypoxic exposure induces immunosuppression which could increase vulnerability to pathogens.
Impact of Lorentz force on the pulsatile flow of a non-Newtonian Casson fluid in a constricted channel using Darcy’s law: a numerical study
Authors: Ali Amjad , Farooq Hamayun , Abbas Zaheer , Bukhari Zainab , Fatima Attia 
Published: June 2020

AbstractThe present paper examines the flow behavior and separation region of a non-Newtonian electrically conducting Casson fluid through a two-dimensional porous channel by using Darcy’s law for the steady and pulsatile flows. The vorticity-stream function approach is employed for the numerical solution of the flow equations. The effects of various emerging parameters on wall shear stress and stream-wise velocity are displayed through graphs and discussed in detail. It is noticed the increasing values of the magnetic field parameter (Hartman number) cause vanishing of the flow separation region and flattening of the stream-wise velocity component. The study also reveals that the non-Newtonian character of Casson fluid bears the potential of controlling the flow separation region in both steady and pulsating flow conditions.
Quantitative analysis of tumor-specific BCL2 expression in DLBCL: refinement of prognostic relevance of BCL2
Authors: Roh Jin , Cho Hyungwoo , Yoon Dok Hyun , Hong Jung Yong , Lee A-Neum , Eom Hyeon Seok , Lee Hyewon , Park Weon Seo , Han Jae Ho , Jeong Seong Hyun , Park Joon Seong , Pak Hyo-Kyung , Kim So-Woon , Kim Sang-Yeob , Suh Cheolwon , Huh Jooryung , Park Chan-Sik 
Published: June 2020

AbstractBCL2 overexpression has been reported to be associated with poor prognosis in patients with diffuse large B-cell lymphoma (DLBCL). However, currently there is no consensus on the evaluation of BCL2 expression and only the proportion of BCL2 positive cells are evaluated for the determination of BCL2 positivity. This study aimed to define BCL2 positivity by quantitative analysis integrating both the intensity and proportion of BCL2 expression. BCL2 expression of 332 patients (221 patients for the training set and 111 patients for the validation set) with newly diagnosed DLBCL who received R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone) were analyzed using the tumor-specific automated quantitative analysis (AQUA) scoring method based on multiplex immunofluorescence. In the training set, high BCL2 AQUA score (N = 86, 38.9%) was significantly associated with poor prognosis (p = 0.01, HR 2.00; 95% CI [1.15–3.49]) independent of international prognostic index, cell of origin, and MYC expression. The poor prognostic impact of the high BCL2 AQUA score was validated in the validation set. AQUA scoring of BCL2 expression incorporating both the intensity and proportion of BCL2 positive cells was independently associated with survival outcomes of patients with primary DLBCL treated with R-CHOP.
Distinctive roles of Abi1 in regulating actin-associated proteins during human smooth muscle cell migration
Authors: Wang Ruping , Liao Guoning , Wang Yinna , Tang Dale D. 
Published: June 2020

AbstractSmooth muscle cell migration is essential for many diverse biological processes such as pulmonary/cardiovascular development and homeostasis. Abi1 (Abelson interactor 1) is an adapter protein that has been implicated in nonmuscle cell migration. However, the role and mechanism of Abi1 in smooth muscle migration are largely unknown. Here, Abi1 knockdown by shRNA reduced human airway smooth muscle cell migration, which was restored by Abi1 rescue. Abi1 localized at the tip of lamellipodia and its protrusion coordinated with F-actin at the leading cell edge of live cells. In addition, we identified profilin-1 (Pfn-1), a G-actin transporter, as a new partner for Abi1. Abi1 knockdown reduced the recruitment of Pfn-1 to the leading cell edge. Moreover, Abi1 knockdown reduced the localization of the actin-regulatory proteins c-Abl (Abelson tyrosine kinase) and N-WASP (neuronal Wiskott–Aldrich Syndrome Protein) at the cell edge without affecting other migration-related proteins including pVASP (phosphorylated vasodilator stimulated phosphoprotein), cortactin and vinculin. Furthermore, we found that c-Abl and integrin β1 regulated the positioning of Abi1 at the leading edge. Taken together, the results suggest that Abi1 regulates cell migration by affecting Pfn-1 and N-WASP, but not pVASP, cortactin and focal adhesions. Integrin β1 and c-Abl are important for the recruitment of Abi1 to the leading edge.
Parvalbumin-, substance P- and calcitonin gene-related peptide-immunopositive axons in the human dental pulp differ in their distribution of varicosities
Authors: Park Sook Kyung , Choi Seung Ki , Kim Youn Gyung , Choi So Young , Kim Jin Wook , Seo Sang Hyeok , Lee Ji Hyun , Bae Yong Chul 
Published: June 2020

AbstractInformation on the frequency and spatial distribution of axonal varicosities associated with release of neurotransmitters in the dental pulp is important to help elucidate the peripheral mechanisms of dental pain, mediated by myelinated versus unmyelinated fibers. For this, we investigated the distribution of axonal varicosities in the human dental pulp using light- and electron-microscopic immunohistochemistry for the vesicular glutamate transporter 2 (VGLUT2), which is involved in the glutamatergic transmission, and syntaxin-1 and synaptosomal nerve-associated protein 25 (SNAP-25), combined with parvalbumin (PV), which is expressed mostly in myelinated axons, and substance P (SP) and calcitonin gene-related peptide (CGRP), which are expressed mostly in unmyelinated axons. We found that the varicosities of the SP- and CGRP-immunopositive (+) axons were uniformly distributed throughout the dental pulp, whereas those of PV+ axons were only dense in the peripheral pulp, and that the expression of PV, VGLUT2, syntaxin-1, SNAP-25, SP and CGRP was significantly higher in the varicosities than in the axonal segments between them. These findings are consistent with the release of glutamate and neuropeptides by axonal varicosities of SP+ and CGRP+ unmyelinated fibers, involved in pulpal pain throughout the human dental pulp, and by varicosities of PV+ fibers, arising from parent myelinated fibers, and involved in dentin sensitivity primarily in the peripheral pulp.
Atelocollagen promotes chondrogenic differentiation of human adipose-derived mesenchymal stem cells
Authors: Kim Seon Ae , Sur Yoo Joon , Cho Mi-La , Go Eun Jeong , Kim Yun Hwan , Shetty Asode Ananthram , Kim Seok Jung 
Published: June 2020

AbstractEffective engineering approaches for cartilage regeneration involve a combination of cells and biomaterial scaffolds. Multipotent mesenchymal stem cells (MSCs) are important sources for cartilage regeneration. Atelocollagen provides a suitable substrate for MSC attachment and enhancing chondrogenic differentiation. Here, we assessed the chondrogenic potential of adipose tissue derived human MSCs (hMSCs) mixed with atelocollagen gel. We observed cell attachment, viability, and microstructures by electron microscopy over 21 days. The levels of Sox9, type II collagen, aggrecan, type I collagen, Runx2, type X collagen, ALP, Osterix, and MMP13 were measured by RT-qPCR. Cartilage matrix-related proteins were assessed by enzyme-linked immunosorbent assay (ELISA), histology, and immunohistochemistry. hMSCs of all groups exhibited well-maintained cell survival, distribution and morphology. Abundant type II collagen fibers developed on day 21; whileSox9, type II collagen, and aggrecan expression increased over time in the atelocollagen group. However, type I collagen,RUNX2, type X collagen (CoL10A1),Osterix, and ALP were not expressed. These results corroborated the protein expression detected by ELISA. Further, histological analysis revealed lacunae-like structures, while staining demonstrated glycosaminoglycan accumulation. Cumulatively, these results indicate that atelocollagen scaffolds improve hMSC chondrogenic differentiation and are a potential approach for cartilage regeneration.
Iron in parasitic protists – from uptake to storage and where we can interfere
Authors: Mach Jan , Sutak Robert 
Published: June 2020

Abstract
It is well known that iron is a crucial micronutrient for all living organisms. Due to its chemical properties, iron is an irreplaceable cofactor of many essential enzymes but is also potentially toxic when present in excess. The acquisition of iron from the environment can be challenging for organisms, especially for parasitic protists that rely solely on the host for available nutrients. One of the host defense mechanisms is to starve parasites by detaining the crucial iron in a form unreachable for pathogens. In this review, we summarize current information about iron homeostasis-related pathways of important human parasites, such as Plasmodium, trypanosomes, Leishmania, pathogenic amoebas and Trichomonas. We focus on the parasites’ strategies of iron acquisition, storage/detoxification, trafficking, and iron-regulated protein expression and address the questions of iron-influenced virulence and anti-parasitic chemotherapeutics targeted to iron metabolism. Finally, we outline the potential of understudied and somewhat neglected iron chelating agents as safe chemotherapeutics against protozoan parasites.
On the nightglow polarisation for space weather exploration
Authors: Bosse Léo , Lilensten Jean , Gillet Nicolas , Rochat Sylvain , Delboulbé Alain , Curaba Stephane , Roux Alain , Magnard Yves , Johnsen Magnar G. , Løvhaug Unni-Pia , Amblard Pierre-Olivier , Le Bihan Nicolas , Nabon Maxime , Marif Hanane , Auriol Frédérique , Noûs Camille 
Published: June 2020

We present here observations of the polarisation of four auroral lines in the auroral oval and in the polar cusp using a new ground polarimeter called Petit Cru. Our results confirm the already known polarisation of the red line, and show for the first time that the three other lines observed here (namely 557.7 nm, 391.4 nm and 427.8 nm) are polarised as well up to a few percent. We show that in several circumstances, this polarisation is linked to the local magnetic activity and to the state of the ionosphere through the electron density measured with EISCAT. However, we also show that the contribution of light pollution from nearby cities via scattering can not be ignored and can play an important role in polarisation measurements. This series of observations questions the geophysical origin of the polarisation. It also leaves open its relation to the magnetic field orientation and to the state of both the upper atmosphere and the troposphere.
Median Nerve Injury in Ultrasound-Guided Hydrodissection and Corticosteroid Injections for Carpal Tunnel Syndrome
Authors: Hsu Yi-Chih , Yang Fu-Chi , Hsu Hsian-He , Huang Guo-Shu 
Published: June 2020

Abstract
Purpose Permanent nerve damage after corticosteroid injection has been suggested when symptoms of median nerve injury (MNI) are irreversible. We assess the outcomes of MNI and their association with ultrasonography (US)-guided hydrodissection and the following corticosteroid injection for symptomatic carpal tunnel syndrome (CTS).
Methods US-guided hydrodissection and the following corticosteroid injections were administered to 126 CTS patients. Occurrence of MNI, clinical data, and post-hydrodissection findings were evaluated. Post-hydrodissection findings included vascular injury during hydrodissection, altered echogenicity, reduced flattening ratio, and increased cross-sectional area of the MN at the inlet of the carpal tunnel (MN-CSA-Inlet) on ultrasonography after hydrodissection. The relevance of MNI with respect to these clinical data and findings was determined. The outcome was rated using Boston Carpal Tunnel Syndrome Questionnaire (BCTQ) scores.
Results Nine patients suffered MNI (incidence, 7.1 %) but improved significantly at follow-up. Clinical data and vascular injury during hydrodissection, altered echogenicity, and reduced flattening ratio after hydrodissection were unrelated to prolonged transient MNI (p > 0.05). MNI was significantly associated with increased CSA (p = 0.005). A CSA increase > 2 mm2 after hydrodissection yielded the greatest performance (0.979) for MNI in the receiver operating characteristic analysis. Decreases in BCTQ scores after injection did not differ significantly between groups with and without MNI (p > 0.05).
Conclusion MNI during hydrodissection may be reversible. MNI is indicated by an increase in MN-CSA-inlet immediately after hydrodissection.
Virtual reality distraction for patients to relieve pain and discomfort during colonoscopy
Authors: Veldhuijzen Govert , Klaassen Nienke J.M. , Van Wezel Richard J.A. , Drenth Joost P.H. , Van Esch Aura A. 
Published: June 2020

Abstract
Background and study aims Colonoscopy is an invasive procedure that may cause patients pain and discomfort. Routine use of sedation, while effective, is expensive and requires logistical planning. Virtual reality (VR) offers immersive, three-dimensional experiences that distract the attention and might comfort patients. We performed a pilot study to investigate the feasibility of VR distraction during colonoscopy.
Patients and methods Adults referred for colonoscopy were considered for inclusion and divided over two groups: with and without VR glasses. The main outcome was patient acceptance of wearing VR glasses during colonoscopy without compromising the technical success of the procedure. Secondary outcomes were patient comfort, pain, and anxiety before, during and after the procedure, using validated patient questionnaires. Patient comments were collected through a qualitative interview.
Results We included 19 patients, 10 of whom were offered VR glasses. All patients accepted VR glasses without prolonging procedural time. No disadvantages of the VR glasses were reported in terms of communication or change of position of the patient. We found that patient comfort, pain, anxiety, and satisfaction in relation to the procedure were similar in both groups. Patients described a pleasant distracting effect using VR glasses.
Conclusion VR glasses during colonoscopy are accepted by patients and do not compromise endoscopic technical success. Patients reported that the VR experience was pleasant and distracting.
COMPARISON OF DENTAL CURRICULUM IN INDIA VERSUS DEVELOPED COUNTRIES
Authors: Rao Lakshmi Nidhi , Hegde Mithra N. , Hegde Priyadarshini , Shetty Chitharanjan 
Published: June 2020

Abstract:As the importance of good dental care becomes increasingly paramount in a globally connected society, it is imperative that the dental curriculum keep up pace with it.This means that the large numbers of Indian dental graduates who pass out every year are in sync with latest in the dental world.Hence this study aims to compare the dental curriculum of the traditional Indian BDS undergraduate program with those of the developed countries (restricted to American and British universities).It goes onto highlight the strengths of each of curriculums studied. Additionally it identifies specific features of other curriculums which could be incorporated into the Indian dental curriculum.
Progress in Local Treatment of Breast Cancer: A Narrative Review
Authors: Cavalcante Francisco Pimentel , Millen Eduardo Camargo , Zerwes Felipe Pereira , Novita Guilherme Garcia 
Published: June 2020

AbstractThe present paper reports on the local treatment of breast cancer from a historical perspective. A search for articles written in English was made in the Medline and EMBASE databases, and 40 papers were selected. Over the past 10 years, various randomized, controlled clinical trials on the local treatment of breast cancer indicated that patients with the same molecular subtype may receive different individualized surgical treatments aimed at optimizing systemic adjuvant therapy. With a view to retaining the gains made in disease-free and overall survival, surgical techniques have advanced from radical surgery to conservative mastectomies, thus reducing sequelae, while adjuvant and neoadjuvant therapies have contributed toward controlling the disease, both distant metastases and local recurrence. Current studies evaluate whether future breast cancer therapy may even succeed in eliminating surgery to the breast and axilla altogether.
The Effectiveness of Psycho-Educational and Cognitive-Behavioral Counseling on Female Sexual Dysfunction
Authors: Mirzaee Firoozeh , Ahmadi Atefeh , Zangiabadi Zahra , Mirzaee Moghaddameh 
Published: June 2020

Abstract
Introduction Sexual function is a multidimensional phenomenon that is affected by many biological and psychological factors. Cognitive-behavioral sex therapies are among the most common nonpharmacological approaches to psychosexual problems. The purpose of the present study was to investigate the effectiveness of psychoeducational and cognitive-behavioral counseling on female sexual dysfunction.
Methods The present study was a clinical trial with intervention and control groups. The study population consisted of women referring to the general clinic of a governmental hospital in Iran. After completing the demographic questionnaire and Female Sexual Function Index (FSFI), those who obtained the cutoff score ≤ 28 were contacted and invited to participate in the study. Convenience sampling method was used and 35 subjects were randomly allocated for each group. Eight counseling sessions were held for the intervention group (two/week/1.5 hour). Post-test was taken from both groups after 1 month, and the results were statistically analyzed by PASW Statistics for Windows, Version 18 (SPSS Inc., Chicago, IL, USA).
Results The total mean scores of FSFI and the subscales of sexual desire, arousal, orgasm, and satisfaction were significantly higher in the intervention group than in the control group after the intervention. In addition, postintervention pain mean scores in the intervention group were significantly lower than in the control group (p < 0.05).
Conclusion The results of the present study indicate that psychoeducational cognitive-behavioral counseling is effective in improving female sexual function. It is recommended to compare the effects of psychoeducational cognitive-behavioral counseling on sexual dysfunctions of couples and with a larger sample size in future research.
Changes in pH as a result of galvanic currents used in percutaneous needle electrolysis
Authors: Margalef Ramón , Minaya-Muñoz Francisco , Valera-Garrido Fermín , Bosque Marc , Santafé Manel M. 
Published: June 2020

Abstract
Aim To determine whether sodium chloride electrolysis causes a change in the pH of tissues.
Methods The effects of a 3 mA galvanic current has been evaluated, applied for 3 seconds and 3 repetitions (3:3:3). In vitro pH changes were evaluated in three experiments: 1) Eppendorf® tubes filled with Ringer's solution; 2) a very small volume of Ringer's solution (100µl); 3) Eppendorf® tubes filled with saline solution (NaCl 0.9%). The pH changes in the gastrocnemius of mice were evaluated, using the left limb as a control and the right limb for the intervention. The gastrocnemius muscles were ground up and the pH of each group was determined.
Results In the in vitro experiments 1 and 2, no variation was observed in the pH of either the cathode in the Ringer's solution or the anode in the Ringer's solution (the variation did not exceed 16% in either of the cases, p> 0.05). In the third in vitro study, the pH after galvanic current application increased by 70% in the saline solution of the cathode and the anode pH decreased by 34% (p < 0.05 in both cases). In the in vivo experiments, no change in pH was obtained (% variation: 0.00 ± 0.00).
Conclusions The galvanic current used in percutaneous needle electrolysis applying the 3:3:3 parameters generates very small changes in the pH, in the area near the needle, which the body is able to rapidly compensate for.