bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality assessment: Qualitative and quantitative analysis of nine active components in Sargentodoxa cuneata by HPLC/Q Exactive Plus MS and UPLC-ESI-MS/MS
Authors: Zheng Lin , Zhou Zu-Ying , Wang Chang-Quan , Wang Yong-Lin , Gong Zi-Peng , Li Yue-Ting , Wang Ai-Min , Chen Si-Ying , Ma Xue , Li Yong-Jun , Huang Yong 
Published: July 2020

Abstract
Background: Sargentodoxa cuneata is an herb commonly used by the Hmong people, and many preparations containing this herb have been marketed. However, the source of medicinal materials is not standardized during the production process, and the quality of medicinal materials from different sources may be inconsistent. This study was aimed to establish a qualitative and quantitative analysis method for quality assessment of Sargentodoxa cuneata in different regions. Methods: HPLC/Q Exactive Plus MS was performed in positive mode and negative mode used for qualitative analysis, and there were twenty-one ingredients were tested. Nine high-content or characteristic ingredients were screened out and applied to the quantitative analysis of thirty batches of medicinal materials from different origins. Based on the results of the quantitative analysis, further multivariate statistical analyses were carried out for quality assessment of Sargentodoxa cuneata . Results: Results showed that there were significant differences in the contents of the nine ingredients of Sargentodoxa cuneata in the eastern and southwestern regions. The content of hydroxytyrosol, salidroside, chlorogenic acid, desrhamnosyl isoacteoside, and liriodendrin of Sargentodoxa cuneata in eastern region was higher, while the content of protocatechin in southwestern region was higher. The different ingredients included protocatechuic acid, desrhamnosyl isoacteoside, liriodendrin, and hydroxytyrosol. These four ingredients were important factors that caused the difference in the quality of Sargentodoxa cuneata from different origins. Conclusion: Protocatechuic acid, desrhamnosyl isoacteoside, liriodendrin, and hydroxytyrosol may become a new marker for quality assessment of Sargentodoxa cuneata.
Characteristics of Microbiome in Lung Adenocarcinoma Tissue from Patients in Southwestern China
Authors: Zhu Sinuo , Zhao Yunping , Bao Yanan , Cui Yue , Zhu Xingming , He Xuan , Ye Mengxiao , Chen Xiaobo 
Published: July 2020

Abstract
Background:Increasing evidences have unveiled the connection between microbiome and lung cancer. This study aims to identify the characteristics of microbial communities in the lung cancer tissues from patients in southwestern China, and to compare the distinct microbial genes at different clinical stages of lung cancer for uncovering potential immunotherapy targets.Methods:Forty samples of primary lung adenocarcinoma tissue were performed by 16S rRNA gene sequencing. The subjects were grouped according to TNM stages (T and N group), clinical stage and smoke status. To identify the taxa composition of each sample, Operational Taxonomic Units (OTUs) were classified on the Effective Tags with 97% identity. The linear discriminant analysis effect size (LEfSe) method was utilized to compare relative abundances of all bacterial taxa between non-metastasis group and metastasis group. The Shannon index of the 97% identity OTUs was calculated to evaluate alpha diversity. Beta diversity measurement was calculated using Principal Co-ordinates Analysis (PCoA).Results:A total of 951 OTUs were identified in the cancer tissues, including 224 overlapping genera. No significant difference has been found in the alpha diversity within all the groups. Beta diversity was significantly different in N group, T group and clinical stage group. By LEfSe analysis, nine differential species were identified in the N group, of which the relative abundance of genus Bifidobacterium was 10.78%±11.59% in the N0 group and 20.15%±13.44% in the N+ group (p<0.05). In the T1 and T2 group, the LEfSe result identified 4 phylum and 10 genera. The differential genera were Moraxella, Dolosigranulum, Corynebacteriaceae and Citrobacter in the T2 group and Bifidobacterium, Alistipes, Akkermansia, Blautia, Lactobacillus as well as Facelibacterium in the T1 group. Differential bacterial composition and abundance were also observed in the clinical stage group.Conclusions:In conclusion, by 16S RNA sequencing, we identified dominant species of lung cancer tissue in different groups of AD patients. Bifidobacterium plays important role both in lymph node metastasis and tumor progression, which could provide specific immunotherapy strategy for lung cancer.
Study of the immunogenicity of recombinant protein consisting of multiple epitopes of porcine epidemic diarrhea virus highly expressed in Sf9 cells
Authors: Liu Yangkun , Hu Xiaomin , Zhang Nannan , Li Na , Guan Zhihan , Burov Andrey , Puzyr Alexey , Bondar Vladimir , Yao Lunguang 
Published: July 2020

Abstract
Background

Current inactivated and attenuated vaccines can not provide complete protection, resulting in the ongoing epidemic of porcine epidemic diarrhea virus (PEDV), which has caused significant economic losses in the swine industry worldwide. To develop an alternative efficient and economical vaccine, a fusion protein named S1CD containing multiple neutralizing epitopes of PEDV was designed and expressed in Sf9 cells. The expression yield was optimized by production of baculoviral constructs harboring multiple S1CD-expressing cassettes, and the immunogenicity of the purified protein was evaluated.
Results

The results showed that high-level expression of the S1CD fusion protein was achieved by multi-copy gene co-expression. The recombinant virus carrying three copies of S1CD gene under the control of p10 promoter showed the highest expression level, with a yield of 462 mg/L, which is about 2.6 or 3.5 fold that of the recombinant viruses carrying only one copy. BALB/c mice were subcutaneously immunized with purified S1CD protein three times at 2 week intervals. After immunization, S1CD protein could induce the mice produce high level of specific IgG antibodies and neutralizing antibodies.
Conclusion

Fusion S1CD protein consisting of multiple epitopes of porcine epidemic diarrhea virus was highly expressed by multi-copy gene co-expression. Moreover, it stimulated mice to produce high level of specific IgG antibodies and neutralizing antibodies. The S1CD protein expressed in this study could be used as a putative economic and efficient subunit vaccine against PEDV infection.
Seasonal Bioavailability of Heavy Metal Contaminants From the University of Lagos Lagoon, Nigeria
Authors: Sowunmi Kehinde , Titilayo Soyebo , Sowunmi Lukman 
Published: July 2020

Abstract
Heavy metals have been implicated as Lagoon pollutants from sources such as agriculture, mining and manufacturing industries and waste water treatment works. A study was carried out in the University of Lagos lagoon to investigate the seasonal bioavailability of the heavy metal contaminants Cd, Cr, Cu, Pb and Zn. The physical parameters pH, redox potential, temperature, TDS and conductivity were measured on site. Dried sediment samples were extracted using the Community Bureau of Reference (BCR) sequential procedure and analysed by ICP-OES. A certified reference material (CRM), BCR 701 (lake sediment) was used for quality assurance with recoveries ranging between 80-120%. Statistical analysis (ANOVA) showed that there was a significant difference between metal distribution in the dry and wet seasons. Cr and Cd were partitioned in the residual fraction (unavailable for uptake). Cu, Pb and Zn were in the available fractions (carbonate, Fe/Mn oxide and organic). Cu was highest in the Fe/Mn oxide and organic fractions. This indicated that an increase in reducing agents and organic matter will avail the Cu. Zn was distributed in all fractions while Pb was found in the Fe/Mn oxide fraction (3.93- 21.3 %). Results showed that the bioavailability of Cu, Pb and Zn was high. Metal bioavailability by BCR indicates a potential risk of pollution in lagoon sediments as the available metals exceeded the permissible Sediment Quality Assessment Guidelines (SQAG’s) from Environmental Protection Agency (EPA).
Leisure-time, occupational, and commuting physical activity and the risk of chronic kidney disease in a working population: a prospective cohort study
Authors: Yamamoto Shohei , Inoue Yosuke , Kuwahara Keisuke , Miki Takako , Nakagawa Tohru , Honda Toru , Yamamoto Shuichiro , Hayashi Takeshi , Mizoue Tetsuya 
Published: July 2020

Abstract
Background:

Very few attempts have been made to examine the association of leisure-time physical activity with chronic kidney disease (CKD) onset. In addition, there is no prospective information on the relationship between other domains of physical activity and CKD. In this study, we examined the risk of CKD in the context of leisure-time, occupational, and commuting physical activity.
Methods:

This prospective cohort study included 17,331 Japanese workers (aged 20–65 years at baseline) without CKD who were followed from 2006 to 2020. Leisure-time, occupational physical activity, and the duration of walking to and from work were assessed by a questionnaire at baseline. Incident CKD was defined as estimated glomerular filtration rate < 60 mL/min/1.73 m2 and/or proteinuria [1+, 2+, or 3+] by dipstick. Cox proportional hazard regression models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) of CKD.
Results:

During 147,752 person-years of follow-up, 4,013 participants developed CKD. After controlling for a wide range of covariates, leisure-time physical activity and walking for commute were not associated with CKD onset. By contrast, compared to the participants mostly engaging in sedentary work, those with standing/walking and fairly active work had HRs of 0.86 (95% CI: 0.79–0.94) and 0.87 (95%CI: 0.76-1.00) to develop CKD, respectively, after adjusting for all covariates including the other forms of physical activity (p for trend = 0.01).
Conclusions:

Occupational physical activity was associated with the risk of CKD, and leisure-time and commuting physical activity were not.
Da Cheng Qi Decoction Alleviates Cerulein-Stimulated AR42J Pancreatic Acinar Cell Injury via the JAK2/STAT3 Signalling Pathway
Authors: Zhou Zehua , Chen Ying , Dong Wenmin , An Rui , Liang Kun , Wang Xinhong 
Published: July 2020

Abstract
Background: Acute pancreatitis (AP) is a common acute abdomen, characterized by the dysregulation of digestive enzyme production and secretion. Many studies have shown that Da Cheng Qi Decoction (DCQD) is a secure, effective prescription on AP. In this study, cerulein-stimulated AR42J cells damage model was established to further explore the feasibility and underlying mechanism of DCQD as a potential JAK2/STAT3 pathway inhibitor for the treatment of AP. Methods: Cell viability of DCQD was measured using a Cell Counting Kit-8 assay. Pancreatic biochemical markers such as amylase, lipase and C-reactive protein production were measured by assay kits respectively. Cytokines (TNF-α, IL-6, IL-10 and IL-1β) were assayed by ELISA. Protein location was detected by immunofluorescence staining. Proteins expression was quantified by Western blotting, and gene expression was assessed by real‑time PCR. For mechanistic analysis of the effect of DCQD on JAK2/STAT3 signaling pathway, selective JAK2 inhibitor (Fedratinib) and STAT3 inhibitor (Stattic) as well as STAT3 activator (Garcinone D) were used.Results: DCQD protected cells by regulating cerulein-induced inflammation and reducing the secretion of pancreatic biochemical markers. The mechanisms mediating these effects may be related to the suppression of the JAK2/STAT3 pathway. Additionally, DCQD significantly attenuated cell injury and regulated the expression of downstream targets Bax and Bcl-XL. Moreover, treatment with JAK2 inhibitor Fedratinib or STAT3 inhibitor Stattic reversed the activated effect of cerulein on JAK2/STAT3 pathway. And the activation of JAK2/STAT3 pathway, via STAT3 activator Garcinone D, did exert damage on cells, which bore a resemblance to cerulein.Conclusion: The activation of JAK2/STAT3 pathway may play a key role in the pathogenesis of cerulein-stimulated AR42J pancreatic acinar cell injury. DCQD could improve inflammatory cytokines and cell injury, which might be mediated by suppressing the activation of JAK2/STAT3 signaling pathway.
An unusual collection: Nasal septal abscess secondary to a Furuncle
Authors: Bhaga Heeral , Maharaj Shivesh 
Published: July 2020

Abstract
Nasal septal abscess is an uncommon entity and most likely to occur with nasal septal hematoma associated with trauma. It occurs when pus collects between the mucoperichondrium and the nasal septal cartilage. Complications can be avoided by prompt identification and initiation of appropriate treatment. Thus avoiding systemic and cosmetic sequelae.We present a septal abscess secondary to nasal furuncle in a tertiary institute. This case report also serves to illustrate the investigations, recommended management and possible treatment outcomes.
No Coil® Placement in Patients Undergoing Left Hemicolectomy and Lower Anterior Resection for Colorectal Cancer: Efficacy, Tolerability and Differences.
Authors: Ammendola Michele , Ruggiero Michele , Talarico Carlo , Memeo Riccardo , Ammerata Giorgio , Capomolla Antonella , Filippo Rosalinda , Romano Roberto , Pallio Socrate , Navarra Giuseppe , Montemurro Severino , Currò Giuseppe 
Published: July 2020

Abstract
Background. Colorectal cancer (CRC) is the most common type of the gastrointestinal tract. Anastomotic leak (AL), is the most important complication of colorectal surgery. In this retrospective study, we evaluated efficacy and tolerability of transanal tube No Coil® in patients with CRC undergoing low anterior resection (LAR) and left hemicolectomy (LC). Methods. Thirty-eight patients operated by the same team for primary colorectal adenocarcinoma were collected, 24 LC and 14 LAR. Neither defuncioning stoma nor neoadjuvant therapy not was administered to anyone patients. No Coil® was placed immediately after the end of surgical treatment.Results. In our experience, LC was performed in 24 individuals (63%), LAR in 14 individuals (37%). AL was evident in 2,63 % of the sample; no statistical difference was found in AL occurrence between LC and LAR with only one patient in LAR group.Conclusion. With our preliminary data, we suggest that No Coil® placement can be considered as a valuable procedure assisting colorectal surgery, but further studies are required to confirm and enlarge actual evidence.
Direct Effects of Nerve Growth Factor-β, Purified From Bull Seminal Plasma,on Steroidogenesis and Angiogenic Markers of Thebovine Pre-ovulatory Follicle
Authors: Stewart Jamie L. , Gao Liying , Flaws Jodi A. , Mercadante Vitor R.G. , Dias Nicholas W. , Canisso Igor F. , Lima Fabio 
Published: July 2020

Abstract
Nerve growth factor-β (NGF) is critical for ovulation in the mammalian ovary and is luteotrophic when administered systemically to camelids and cattle. This study aimed to assess the direct effects of purified bovine NGF on steroidogenesis and angiogenic markers in the bovine pre-ovulatory follicle. A chort of Holstein heifers were synchronized with a standard protocol and heifers with the preovulatory follicle (≥ 12 mm) had an the ovary containing the dominant follicle removed via colpotomy. Pre-ovulatory follicles were dissected in 24 pieces containing theca and granulosa cells that were randomly allocated to receive either cultured in media supplemented with purified bovine NGF (100 ng/mL) or untreated (control) for 72 h. The supernatant media was harvested for determination of progesterone, testosterone, and estradiol, whereas explants were used for mRNA analyses for steroidogenesis and angiogenic markers. Treatment of follicle tissue with NGF upregulated gene expression of steroidogenic enzyme HDS17B ( P = 0.04) and increased testosterone production ( P < 0.01). However, NGF treatment did not alter production of progesterone ( P = 0.81) or estradiol ( P = 0.14). Consistently, gene expression of steroidogenic enzymes responsible for producing these hormones ( STAR , CYP11A1 , HSD3B , CYP17A1 , CYP19A1 ) were unaffected by NGF treatment ( P ≥ 0.31). Treatment with NGF downregulated gene expression of the angiogenic enzyme FGF2 ( P = 0.02) but did not alter PGES ( P = 0.63), VEGFA ( P = 0.44), and ESR1 ( P = 0.77). Collectively, these results demonstrate that NGF from seminal plasma may interact directly with the bovine pre-ovulatory follicle to alter downstream steroidogenesis and luteal development.
Antibiotic-resistant staphylococci from the wastewater treatment plant and grey-water samples in Obafemi Awolowo University, Ile-Ife, Nigeria
Authors: Adesoji Tomiwa Olumide , Egyir Beverly , Shittu Adebayo Osagie 
Published: July 2020

Abstract
This study examined the occurrence and molecular basis for antibiotic-resistant staphylococci from the wastewater treatment plant and grey-water samples in Obafemi Awolowo University, Nigeria. Standard microbiological techniques and molecular methods were utilized. The species identified (MALDI score &gt;1.7) comprised S. saprophyticus (19), S. cohnii (8), S. sciuri (7), S. aureus (4), S. epidermidis (3), S. warneri (2), S. equorum (1), S. haemolyticus (1), S. nepalensis (1), S. condimenti (1), and S. pasteuri (1). Resistance to trimethoprim, tetracycline and cefoxitin were observed in 78.3% (47/60), 36.7% (22/60) and 25% (15/60) of the isolates, respectively. The rate of multidrug resistance was 53.3% (32/60) and observed in eight species from different sampling sites. Seven (S. sciuri; n = 5; S. aureus; n = 1; S. warneri; n = 1) of the 20 selected (representing the various staphylococcal species and antibiotypes) isolates were mecA-positive. Furthermore, the tetK gene was detected in nine isolates, six with dfrA, and four were positive for the dfrG gene. One S. aureus was mecA, tetK and dfrG gene positive. The study provides insights on antibiotic-resistant staphylococci from a non-clinical setting and highlights the need for active surveillance to understand the burden of antimicrobial resistance in Nigeria. This is key to improve synergy across the human, animal and environmental health sectors in Nigeria.
This article has been made Open Access thanks to the generous support of a global network of libraries as part of the Knowledge Unlatched Select initiative.
Application of solid phase extraction (SPE) coupled to dispersive liquid–liquid micro-extraction (DLLME) and sensory evaluation technique for the study of taste and odor active compounds in water
Authors: Zou Jian , Shi Chenyi , Xu Jingyi , Green Shayne , Zhou Yang , Yang XiuHan , Fry Tanya , Winniford Bill 
Published: July 2020

Abstract
Short-chain carbonyls could cause taste and odor issues in drinking water due to their very low odour threshold concentrations (OTCs). Several techniques such as 2,4-dinitrophenylhydrazine (DNPH) derivatization-HPLC and solid-phase micro-extraction (SPME) have been applied for trace short-chain aldehydes analysis in water. However, to study the taste and odor contributors in drinking water, it would be helpful to obtain an extract with representative odor from water samples for further studies, such as GC-olfactometry analysis or sensory test. Therefore, the above techniques still have some constraints due to the derivatization process or complex operating procedures. In this work, we developed a solid-phase extraction (SPE) coupled to dispersive liquid–liquid micro-extraction (DLLME) method to study short-chain aldehydes in ozonated water samples (LOQ = 1 ppb, RSD% = 12–45%). This method enables us to obtain an extract with representative odor from water for further volatile components analysis and sensory evaluation. Pair-wise nose clip sensory evaluation on the taste and odor of ozonated water indicated a high correlation between odor and taste of ozonated water. The SPE-DLLME method enabled a reliable study, for the first time, on correlations of volatile carbonyls and taste & odor performance for ozonated water by applying a series of statistical models.
Removal of the antimicrobial activity from fortified effluents with fluoroquinolones by photocatalytic processes: a comparative study of differently synthesized TiO2-N
Authors: Venancio Wilson Augusto Lima , Rodrigues-Silva Caio , Spina Mylena , Guimarães José Roberto 
Published: July 2020

Abstract
This study presents a comparison of three methods for TiO2-N synthesis that were applied in the photocatalytic oxidation of the fluoroquinolones (FQs) ciprofloxacin, ofloxacin, and lomefloxacin in aqueous solution. The TiO2-N bandgap is small enough to allow the use of solar energy in the photocatalytic oxidation (PCO) reactions. The TiO2 doped by a sol-gel method with titanium butoxide (TiO2–N–BUT) and titanium isopropoxide (TiO2–N–PROP) as the precursor were effective as the TiO2 (P25) impregnation with urea (TiO2-N-P25) to degrade the FQs. The FQ degradation was higher by 74, 65, and 91%, respectively for TiO2–N–BUT, TiO2–N–PROP, and TiO2-N (load 50 mg L−1, 20 min of reaction under 28 W UV-ASolar). The TiO2-P25 with urea showed the best performance in FQ degradation. The reaction intermediates might present modifications in their acceptor groups by PCO and, because of that the antimicrobial activity dropped as the reaction time increased. Reactions with TiO2-N-P25 (100 mg L−1) and TiO2-N-BUT (100 mg L−1) achieved ≥ 80% of antimicrobial activity removal from the mixed FQ solution (Cciprofloxacin = 100 μg L−1; Cofloxacin = 100 μg L−1; Clomefloxacin = 100 μg L−1) after 40 min of reaction, for both for Escherichia coli and Bacillus subtilis.
Bioproducts characterization of residual periphytic biomass produced in an algal turf scrubber (ATS) bioremediation system
Authors: de Souza Maiara P. , Rizzetti Tiele M. , Hoeltz Michele , Dahmer Mainara , Júnior João A. , Alves Gisele , Benitez Lisianne B. , Schneider Rosana C. S. 
Published: July 2020

Abstract
The transformation of residual biomass from bioremediation processes into new products is a worldwide trend driven by economic, environmental and social gain. The present study aimed to evaluate the potential for obtaining bioproducts of technological interest from the remaining periphytic biomass formed during a bioremediation process with an algal turf scrubber (ATS) system installed in a lake catchment. Different methodologies were used according to the target bioproduct. Analyses were performed by high performance liquid chromatography with diode array detector (HPLC/DAD), gas chromatography mass spectrometry (GC-MS), ultraviolet–visible spectroscopy (UV-VIS) and inductively coupled plasma optical emission spectrometry (ICP-OES). The results demonstrated that the periphytic biomass presented potential since protein (17.7%), carbohydrates (22.4%), total lipids (3.3%) with 3.6 mg mL−1 of fatty acids, antioxidants (144.5 μmol Trolox eq. g−1) and chlorophyll a, chlorophyll b and carotenoids (1,719.7 μg mL−1, 541.2 μg mL−1 and 317.7 μg mL−1, respectively) were obtained. Inorganic analysis presented a value of 42.3 ± 2.58% of total ash and metal presence was detected, indicating bioaccumulation. The properties found in periphyton strengthen the possibility of its application in different areas, ensuring bioremediation efficiency.