bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a lateral flow test for bed bug detection
Authors: Ko Alexander , Choe Dong-Hwan 
Published: August 2020

AbstractLateral flow strip tests are a cost-effective method for detecting specific proteins in biological samples, which can be performed in the field without specialized expertise. While most recognizable in the pregnancy tests, there are many other applications for lateral flow strip technology. The pest control industry has increasingly emphasized the importance of pest monitoring to reduce unnecessary applications, focus interventions into locations with active pest infestations, and to develop records of pest infestation. Due to their cryptic behavior, the detection of bed bugs often necessitates labor-intensive, time-consuming and invasive visual inspections. A lateral flow strip test for the detection of bed bugs would represent a novel use for a well-established technology, which can enable pest control operators to rapidly confirm the presence or absence of bed bugs in a room. In the current report, we present an effort to develop and calibrate the lateral flow test devices for the detection of a bed bug specific protein. A variety of bed bug residue samples were prepared by varying several parameters: bed bug infestation level (1 bed bug/3 bed bugs), surface type (wood/fabric), feeding status (fed/unfed), and bed bug time-on-surface (1 d/7 d). Using a prototype sensor and test strip, we examined how these variables influenced the detection of the bed bug specific proteins in the sample and to what degree. We discuss how this lateral flow test device can be an effective tool to determine the presence or absence of bed bug proteins on a surface, providing highly credible evidence on bed bug infestations.
Morin protects the blood–brain barrier integrity against cerebral ischemia reperfusion through anti-inflammatory actions in rats
Authors: Khamchai Satchakorn , Chumboatong Wijitra , Hata Janejira , Tocharus Chainarong , Suksamrarn Apichart , Tocharus Jiraporn 
Published: August 2020

AbstractThis study aimed to investigate the effects of morin on cerebral damage and blood–brain barrier (BBB) integrity in a middle cerebral artery occlusion (MCAO) and reperfusion model. Wistar rats were exposed to MCAO for 2 h, followed by reperfusion. Thirty mg/kg of morin was administered via intraperitoneal injection at the different time points: before ischemia, during ischemia, and at reperfusion. The rats were divided into five groups, including sham, vehicle, and three groups of morin. Twenty-four hours after reperfusion, the rats were tested for neurological deficits, and the brains were harvested to assess brain damage. In addition, brains were harvested 72 h to determine BBB disruption. We found that morin significantly reduced reactive oxygen species production and lipid peroxidation. It also decreased inflammation via reducing the expression of Toll-like receptor 4, nuclear factor kappa-beta. Morin ameliorated cerebral damage and reduced apoptosis through decreasing the cerebral infarct size, including apoptotic cell death. Moreover, morin decreased the BBB damage via reducing Evans blue extravasation, neutrophil infiltration, and increasing tight junction protein expression. Therefore, morin protected against cerebral and BBB damage by attenuating oxidative stress, inflammation, and apoptosis in MCAO and reperfusion models.
Circulating GFAP and Iba-1 levels are associated with pathophysiological sequelae in the thalamus in a pig model of mild TBI
Authors: Lafrenaye Audrey D. , Mondello Stefania , Wang Kevin K. , Yang Zhihui , Povlishock John T. , Gorse Karen , Walker Susan , Hayes Ronald L. , Kochanek Patrick M. 
Published: August 2020

AbstractSerum biomarkers are promising tools for evaluating patients following traumatic brain injury (TBI). However, their relationship with diffuse histopathology remains unclear. Additionally, translatability is a focus of neurotrauma research, however, studies using translational animal models are limited. Here, we evaluated associations between circulating biomarkers and acute thalamic histopathology in a translational micro pig model of mTBI. Serum samples were collected pre-injury, and 1 min-6 h following mTBI. Markers of neuronal injury (Ubiquitin Carboxy-terminal Hydrolase L1 [UCH-L1]), microglial/macrophage activation (Ionized calcium binding adaptor molecule-1 [Iba-1]) and interleukin-6 [IL-6]) and astrogliosis/astrocyte damage (glial fibrillary acidic protein [GFAP]) were measured. Axonal injury and histological features of neurons and glia were also investigated using immunofluorescent labeling and correlated to serum levels of the associated biomarkers. Consistent with prior experimental and human studies, GFAP, was highest at 6 h post-injury, while no substantial changes were observed in UCH-L1, Iba-1 or IL-6 over 6 h. This study also found promising associations between thalamic glial histological signatures and ensuing release of Iba-1 and GFAP into the circulation. Our findings suggest that in diffuse injury, monitoring serum Iba-1 and GFAP levels can provide clinically relevant insight into the underlying acute pathophysiology and biomarker release kinetics following mTBI, providing previously underappreciated diagnostic capability.
Coupling day length data and genomic prediction tools for predicting time-related traits under complex scenarios
Authors: Jarquin Diego , Kajiya-Kanegae Hiromi , Taishen Chen , Yabe Shiori , Persa Reyna , Yu Jianming , Nakagawa Hiroshi , Yamasaki Masanori , Iwata Hiroyoshi 
Published: August 2020

AbstractGenomic selection (GS) has proven to be an efficient tool for predicting crop-rank performance of untested genotypes; however, when the traits have intermediate optima (phenology stages), this implementation might not be the most convenient. GS might deliver high-rank correlations but incurring in serious bias. Days to heading (DTH) is a crucial development stage in rice for regional adaptability with a significant impact on yield potential. The objective of this research consisted in develop a novel method that accurately predicts time-related traits such as DTH in unobserved environments. For this, we propose an implementation that incorporates day length information (DL) in the prediction process for two relevant scenarios: CV0, predicting tested genotypes in unobserved environments (C method); and CV00, predicting untested genotypes in unobserved environments (CB method). The use of DL has advantages over weather data since it can be determined in advance just by knowing the location and planting date. The proposed methods showed that DL information significantly helps to improve the predictive ability of DTH in unobserved environments. Under CV0, the C method returned a root-mean-square error (RMSE) of 3.9 days, a Pearson correlation (PC) of 0.98 and the differences between the predicted and observed environmental means (EMD) ranged between -4.95 and 4.67 days. For CV00, the CB method returned an RMSE of 7.3 days, a PC of 0.93 and the EMD ranged between -6.4 and 4.1 days while the conventional GS implementation produced an RMSE of 18.1 days, a PC of 0.41 and the EMD ranged between -31.5 and 28.7 days.
Automata representation of successful strategies for social dilemmas
Authors: Murase Yohsuke , Baek Seung Ki 
Published: August 2020

AbstractIn a social dilemma, cooperation is collectively optimal, yet individually each group member prefers to defect. A class of successful strategies of direct reciprocity were recently found for the iterated prisoner’s dilemma and for the iterated three-person public-goods game: By a successful strategy, we mean that it constitutes a cooperative Nash equilibrium under implementation error, with assuring that the long-term payoff never becomes less than the co-players’ regardless of their strategies, when the error rate is small. Although we have a list of actions prescribed by each successful strategy, the rationale behind them has not been fully understood for the iterated public-goods game because the list has hundreds of entries to deal with every relevant history of previous interactions. In this paper, we propose a method to convert such history-based representation into an automaton with a minimal number of states. Our main finding is that a successful strategy for the iterated three-person public-goods game can be represented as a 10-state automaton by this method. In this automaton, each state can be interpreted as the player’s internal judgement of the situation, such as trustworthiness of the co-players and the need to redeem oneself after defection. This result thus suggests a comprehensible way to choose an appropriate action at each step towards cooperation based on a situational judgement, which is mapped from the history of interactions.
Ultrasound-modulated optical glucose sensing using a 1645 nm laser
Authors: Park Eun-Yeong , Baik Jinwoo , Kim Hyojin , Park Sung-Min , Kim Chulhong 
Published: August 2020

AbstractRegular and frequent blood glucose monitoring is vital in managing diabetes treatment plans and preventing severe complications. Because current invasive techniques impede patient compliance and are not infection-free, many noninvasive methods have been proposed. Among them, optical methods have drawn much attention for their rich optical contrast, but their resolution is degraded in deep tissue. Here, we present an ultrasound-modulated optical sensing (UOS) technique to noninvasively monitor glucose that uses an infrared laser (1645 nm) and a single-element focused ultrasound transducer. Focused ultrasound waves can acoustically localize diffused photons in scattering media, and thus optical contrast can be represented with much enhanced spatial resolution. To maximize the signal-to-noise ratio, we compared the modulation depths of UOS signals in both continuous and burst ultrasound transmission modes. Finally, UOS measurements of various glucose concentrations are presented and compared with those acquired in phantoms with a conventional diffuse optical sensing method. The UOS measurements in a 20 mm thick tissue-mimicking phantom show 26.6% accuracy in terms of mean absolute relative difference (MARD), which indicates the great potential of the proposed technique as a noninvasive glucose sensor.
Genetic heterogeneity and prognostic impact of recurrent ANK2 and TP53 mutations in mantle cell lymphoma: a multi-centre cohort study
Authors: Jeong Seri , Park Yu Jin , Yun Woobin , Lee Seung-Tae , Choi Jong Rak , Suh Cheolwon , Jo Jae-Cheol , Cha Hee Jeong , Jeong Jee-Yeong , Chang HeeKyung , Cha Yoon Jin , Kim Hyerim , Park Min-Jeong , Song Wonkeun , Cho Eun-Hae , Jeong Eun-Goo , Lee Junnam , Park Yongmin , Lee Yong Seok , Kim Da Jung , Lee Ho Sup 
Published: August 2020

AbstractThe molecular features of mantle cell lymphoma (MCL), including its increased incidence, and complex therapies have not been investigated in detail, particularly in East Asian populations. In this study, we performed targeted panel sequencing (TPS) and whole-exome sequencing (WES) to investigate the genetic alterations in Korean MCL patients. We obtained a total of 53 samples from MCL patients from five Korean university hospitals between 2009 and 2016. We identified the recurrently mutated genes such as SYNE1, ATM, KMT2D, CARD11, ANK2, KMT2C, and TP53, which included some known drivers of MCL. The mutational profiles of our cohort indicated genetic heterogeneity. The significantly enriched pathways were mainly involved in gene expression, cell cycle, and programmed cell death. Multivariate analysis revealed that ANK2 mutations impacted the unfavourable overall survival (hazard ratio [HR] 3.126; P = 0.032). Furthermore, TP53 mutations were related to worse progression-free survival (HR 7.813; P = 0.043). Among the recurrently mutated genes with more than 15.0% frequency, discrepancies were found in only 5 genes from 4 patients, suggesting comparability of the TPS to WES in practical laboratory settings. We provide the unbiased genetic landscape that might contribute to MCL pathogenesis and recurrent genes conferring unfavourable outcomes.
Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis
Authors: Fass Mónica I. , Rivarola Máximo , Ehrenbolger Guillermo F. , Maringolo Carla A. , Montecchia Juan F. , Quiroz Facundo , García-García Francisco , Blázquez Joaquín Dopazo , Hopp H. Esteban , Heinz Ruth A. , Paniego Norma B. , Lia Verónica V. 
Published: August 2020

AbstractSclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotinia-sunflower pathosystem by analysing temporal changes in gene expression in one susceptible and two tolerant inbred lines (IL) inoculated with the pathogen under field conditions. Differential expression analysis showed little overlapping among ILs, suggesting genotype-specific control of cell defense responses possibly related to differences in disease resistance strategies. Functional enrichment assessments yielded a similar pattern. However, all three ILs altered the expression of genes involved in the cellular redox state and cell wall remodeling, in agreement with current knowledge about the initiation of plant immune responses. Remarkably, the over-representation of long non-coding RNAs (lncRNA) was another common feature among ILs. Our findings highlight the diversity of transcriptional responses to SHR within sunflower breeding lines and provide evidence of lncRNAs playing a significant role at early stages of defense.
The integral spliceosomal component CWC15 is required for development in Arabidopsis
Authors: Slane Daniel , Lee Cameron H. , Kolb Martina , Dent Craig , Miao Yingjing , Franz-Wachtel Mirita , Lau Steffen , Maček Boris , Balasubramanian Sureshkumar , Bayer Martin , Jürgens Gerd 
Published: August 2020

AbstractEfficient mRNA splicing is a prerequisite for protein biosynthesis and the eukaryotic splicing machinery is evolutionarily conserved among species of various phyla. At its catalytic core resides the activated splicing complex Bact consisting of the three small nuclear ribonucleoprotein complexes (snRNPs) U2, U5 and U6 and the so-called NineTeen complex (NTC) which is important for spliceosomal activation. CWC15 is an integral part of the NTC in humans and it is associated with the NTC in other species. Here we show the ubiquitous expression and developmental importance of the Arabidopsis ortholog of yeast CWC15. CWC15 associates with core components of the Arabidopsis NTC and its loss leads to inefficient splicing. Consistent with the central role of CWC15 in RNA splicing, cwc15 mutants are embryo lethal and additionally display strong defects in the female haploid phase. Interestingly, the haploid male gametophyte or pollen in Arabidopsis, on the other hand, can cope without functional CWC15, suggesting that developing pollen might be more tolerant to CWC15-mediated defects in splicing than either embryo or female gametophyte.
Novel behavior in a polymer solution: the disappearance of the melting temperature (Tm) and enthalpy change (ΔHm) of the solvent
Authors: Kim Mi Rae , Park Hee Jung , Cheon Kang Ho , Yeom Choong Kyun , Lee Kee Yoon 
Published: August 2020

AbstractThe phase change temperature and enthalpy change as a function of polystyrene (PS) concentration in dimethylformamide through a dynamic heating and quenching process were investigated. Cold crystallization, freezing and melting phenomena in a 10 wt% PS solution were all observed. Cold crystallization and melting phenomena were still observed in a 20 wt% solution. In a 30 wt% solution, all three phenomena disappeared without any solvent enthalpy changes, e.g., enthalpy changes at the melting temperature. The disappearance of both the melting temperature and the melting enthalpy change indicated that all polymer and solvent molecules in the 30 wt% solution existed only in the amorphous phase without any phase changes despite repeated heating and quenching processes. Thus, our results can provide a new approach for gelation through enthalpy changes and can be applied in the fabrication of porous membranes with a narrow distribution.
Self-reported difficulty initiating sleep and early morning awakenings are associated with nocturnal diastolic non-dipping in older white Swedish men
Authors: Tan Xiao , Lind Lars , Ingelsson Martin , Sundström Johan , Cedernaes Jonathan , Benedict Christian 
Published: August 2020

AbstractChronically blunted nocturnal blood pressure (BP) dipping has been shown to increase the future risk of cardiovascular diseases. In the present cross-sectional study, we investigated whether self-reported insomnia symptoms were associated with an altered 24-h BP profile and blunted nocturnal BP dipping (night-to-day BP ratio > 0.90) in older men. For the analysis, we used 24-h ambulatory blood pressure data and reports of insomnia symptoms (difficulty initiating sleep, DIS; and early morning awakenings, EMA) from 995 Swedish men (mean age: 71 years). Compared to men without DIS, those reporting DIS (10% of the cohort) had a higher odds ratio of diastolic non-dipping (1.85 [1.15, 2.98], P = 0.011). Similarly, men who reported EMA (19% of the cohort) had a higher odds ratio of diastolic non-dipping than those without EMA (1.57 [1.09, 2.26], P = 0.015). Despite a slightly higher nocturnal diastolic BP among men with EMA vs. those without EMA (+ 1.4 mmHg, P = 0.042), no other statistically significant differences in BP and heart rate were found between men with and those without insomnia symptoms. Our findings suggest that older men reporting difficulty initiating sleep or early morning awakenings may have a higher risk of nocturnal diastolic non-dipping. Our findings must be replicated in larger cohorts that also include women.
Chemical carving lithography with scanning catalytic probes
Authors: Ki Bugeun , Kim Kyunghwan , Choi Keorock , Oh Jungwoo 
Published: August 2020

AbstractThis study introduces a new chemical carving technique as an alternative to existing lithography and etching techniques. Chemical carving incorporates the concept of scanning probe lithography and metal-assisted chemical etching (MaCE). A catalyst-coated probe mechanically scans a Si substrate in a solution, and the Si is chemically etched into the shape of the probes, forming pre-defined 3D patterns. A metal catalyst is used to oxidize the Si, and the silicon oxide formed is etched in the solution; this local MaCE reaction takes place continuously on the Si substrate in the scanning direction of probes. Polymer resist patterning for subsequent etching is not required; instead, scanning probes pattern the oxidation mask directly and chemical etching of Si occurs concurrently. A prototype that drives the probe with an actuator was used to analyze various aspects of the etching profiles based on the scanning speeds and sizes of the probe used. This technique suggests the possibility of forming arbitrary structures because the carving trajectory is formed according to the scan direction of the probes.
Cerebral tissue pO2 response to treadmill exercise in awake mice
Authors: Moeini Mohammad , Cloutier-Tremblay Christophe , Lu Xuecong , Kakkar Ashok , Lesage Frédéric 
Published: August 2020

AbstractWe exploited two-photon microscopy and Doppler optical coherence tomography to examine the cerebral blood flow and tissue pO2 response to forced treadmill exercise in awake mice. To our knowledge, this is the first study performing both direct measure of brain tissue pO2 during acute forced exercise and underlying microvascular response at capillary and non-capillary levels. We observed that cerebral perfusion and oxygenation are enhanced during running at 5 m/min compared to rest. At faster running speeds (10 and 15 m/min), decreasing trends in arteriolar and capillary flow speed were observed, which could be due to cerebral autoregulation and constriction of arterioles in response to blood pressure increase. However, tissue pO2 was maintained, likely due to an increase in RBC linear density. Higher cerebral oxygenation at exercise levels 5–15 m/min suggests beneficial effects of exercise in situations where oxygen delivery to the brain is compromised, such as in aging, atherosclerosis and Alzheimer Disease.
Quantifying the thermodynamics of protein unfolding using 2D NMR spectroscopy
Authors: Puglisi Rita , Brylski Oliver , Alfano Caterina , Martin Stephen R. , Pastore Annalisa , Temussi Piero A. 
Published: August 2020

AbstractA topic that has attracted considerable interest in recent years is the possibility to perform thermodynamic studies of proteins directly in-cell or in complex environments which mimic the cellular interior. Nuclear magnetic resonance (NMR) could be an attractive technique for these studies but its applicability has so far been limited by technical issues. Here, we demonstrate that 2D NMR methods can be successfully applied to measure thermodynamic parameters provided that a suitable choice of the residues used for the calculation is made. We propose a new parameter, named RAD, which reflects the level of protection of a specific amide proton in the protein core and can guide through the selection of the resonances. We also suggest a way to calibrate the volumes to become independent of technical limitations. The methodology we propose leads to stability curves comparable to that calculated from CD data and provides a new tool for thermodynamic measurements in complex environments.
High-pressure annealing driven nanocrystal formation in Zr50Cu40Al10 metallic glass and strength increase
Authors: Shibazaki Yuki , Yamada Rui , Saida Junji , Kono Yoshio , Wakeda Masato , Itoh Keiji , Nishijima Masahiko , Kimoto Koji 
Published: August 2020

AbstractPressure-induced structural changes in metallic glasses have been of great interest as they are expected to open new ways to synthesize novel materials with unexpected properties. Here, we investigated the effect of simultaneous high-pressure and high-temperature treatment on the structure and properties of a Zr50Cu40Al10 metallic glass by in situ X-ray structure measurement and property analysis of the final material. We found the unusual formation of Cu-rich nanocrystals at high pressure and temperature, accompanied by significant strength and hardness enhancement. Based on reverse Monte Carlo modeling and molecular dynamics simulations, the structure of the metallic glass changed to a densely packed, chemically uniform configuration with high short-range and medium-range ordering at high pressure and temperature. These results show that high-pressure annealing processes provide a new way to improve and control properties without changing their composition.
Yoga as Therapy for People With Aphasia
Authors: Dietz Aimee , Duncan E. Susan , Bislick Lauren , Stegman Sarah , Collins Jenna , Mamlekar Chitrali , Gleason Rachel , McCarthy Michael J. 
Published: August 2020

PurposeThis viewpoint offers a perspective on the potential impact of an adapted yoga program for people with stroke-induced aphasia, with a call for additional work in this area.ConclusionAphasia often results in decreased quality of life (QoL) from fewer social interactions, relationship strain between survivors and co-survivors, depression, and a multitude of other factors. We suggest a therapeutic yoga program for survivors and co-survivors could enhance several components of the dyad's lives that are frequently diminished as a result of aphasia, ultimately increasing QoL. In particular, we highlight the role of resilience and coping as essential tools on the rehabilitative journey in aphasia. After exploring yoga and other mind–body practices, we describe documented positive changes—including cognitive function, social integration, and QoL—following use of yoga in other chronic conditions. As people with communication deficits are typically excluded from these studies, further research is needed to establish whether these benefits generalize to people with aphasia.