bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growth phase proteomics of the heterotrophic marine bacterium Ruegeria pomeroyi
Authors: Krayushkina Dasha , Timmins-Schiffman Emma , Faux Jessica , May Damon H. , Riffle Michael , Harvey H. Rodger , Nunn Brook L. 
Published: December 2019

AbstractThe heterotrophic marine bacterium, Ruegeria pomeroyi, was experimentally cultured under environmentally realistic carbon conditions and with a tracer-level addition of 13C-labeled leucine to track bacterial protein biosynthesis through growth phases. A combination of methods allowed observation of real-time bacterial protein production to understand metabolic priorities through the different growth phases. Over 2000 proteins were identified in each experimental culture from exponential and stationary growth phases. Within two hours of the 13C-labeled leucine addition, R. pomeroyi significantly assimilated the newly encountered substrate into new proteins. This dataset provides a fundamental baseline for understanding growth phase differences in molecular physiology of a cosmopolitan marine bacterium.
Species occurrence of ticks in South America, and interactions with biotic and abiotic traits
Authors: Estrada-Peña Agustin , Nava Santiago , Tarragona Evelina , Bermúdez Sergio , de la Fuente José , Domingos Ana , Labruna Marcelo , Mosqueda Juan , Merino Octavio , Szabó Matias , Venzal Jose M. , Guglielmone Alberto A. 
Published: December 2019

AbstractThe datasets of records of the distribution of ticks and their hosts are invaluable tools to understand the phylogenetic patterns of evolution of ticks and the abiotic traits to which they are associated. Such datasets require an exhaustive collection of bibliographical references. In most cases, it is necessary the confirmation of reliable identification of ticks, together with an update of the scientific names of the vertebrate hosts. These data are not easily available, because many records were published in the so-called “grey literature”. Herein, we introduced the Dataset of Ticks in South America, a repository that collates data on 4,764 records of ticks (4,124 geo-referenced) with a special reference to an extra 2,370 records of ticks on cattle, together with a set of abiotic traits, curated from satellite-derived information over the complete target region. The dataset includes details of the phylogenetic relationships of the species of hosts, providing researchers with both biotic and abiotic traits that drive the distribution and evolution of ticks in South America.
A recombination bin-map identified a major QTL for resistance to Tomato Spotted Wilt Virus in peanut (Arachis hypogaea)
Authors: Agarwal Gaurav , Clevenger Josh , Kale Sandip M. , Wang Hui , Pandey Manish K. , Choudhary Divya , Yuan Mei , Wang Xingjun , Culbreath Albert K. , Holbrook C. Corley , Liu Xin , Varshney Rajeev K. , Guo Baozhu 
Published: December 2019

AbstractTomato spotted wilt virus (TSWV) is a devastating disease to peanut growers in the South-eastern region of the United States. Newly released peanut cultivars in recent years are crucial as they have some levels of resistance to TSWV. One mapping population of recombinant inbred line (RIL) used in this study was derived from peanut lines of SunOleic 97R and NC94022. A whole genome re-sequencing approach was used to sequence these two parents and 140 RILs. A recombination bin-based genetic map was constructed, with 5,816 bins and 20 linkage groups covering a total length of 2004 cM. Using this map, we identified three QTLs which were colocalized on chromosome A01. One QTL had the largest effect of 36.51% to the phenotypic variation and encompassed 89.5 Kb genomic region. This genome region had a cluster of genes, which code for chitinases, strictosidine synthase-like, and NBS-LRR proteins. SNPs linked to this QTL were used to develop Kompetitive allele specific PCR (KASP) markers, and the validated KASP markers showed expected segregation of alleles coming from resistant and susceptible parents within the population. Therefore, this bin-map and QTL associated with TSWV resistance made it possible for functional gene mapping, map-based cloning, and marker-assisted breeding. This study identified the highest number of SNP makers and demonstrated recombination bin-based map for QTL identification in peanut. The chitinase gene clusters and NBS-LRR disease resistance genes in this region suggest the possible involvement in peanut resistance to TSWV.
Fractal Analysis in Pore Size Distributions of Different Bituminous Coals
Authors: Zhu Jie , He Fa , Zhang Yang , Zhang Rui , Zhang Bo 
Published: December 2019

AbstractCoal bumps, coal and gas bursts are currently the main threats to coal mine safety in China. The physical properties of coal are important determining factors for the occurrence of coal bumps or coal and gas bursts. A series of experiments using mercury intrusion porosimetry (MIP), nitrogen adsorption (NA) and carbon dioxide adsorption (CA) were employed to investigate the pore size distributions (PSDs) of bump-prone coal and gas-outburst coal. Considering the influence of coal matrix compressibility on the MIP experimental data, the MIP data should be considered in combination with NA and CA testing data. The dominant pores of gas-outburst coal are different from those of bump-prone coal. The PSDs of coal samples have multifractal characteristics. However, the multifractal characteristics of two types of coal are different. (Answer to question 1, reviewer 2). A comparison of the multifractal parameters indicated that Xin Zhou Yao (XZY) coal samples have a higher spatial heterogeneity and complexity of their pore size distribution, while Zhao Ge Zhuang (ZGZ) coal samples have a lower heterogeneity and pore connectivity, which may hinder smooth gas flow and lead to a localized collection of gas in coal seams.
Daily dynamic performance of a solar chimney power plant integrated by waste heat recovery
Authors: Zhou Xinping , Xu Yangyang 
Published: December 2019

The numerical simulation of a large‐scale hybrid SCPP (an additional waste heat source is connected to the collector‐to‐chimney transition section) is performed based on a transient model. The daily dynamic profiles of power output, mass flow rate of condensed water and temperature at a representative point for continuous 24 h are obtained, as well as the daily total electricity output and the power difference‐to‐average ratio (PDAR) defined as the ratio of the peak‐valley difference of the daily power profile to the daily average. The effect of effective waste heat flux (EWHF) on the power output by changing the solar radiation intensity is evaluated. Results show that the incorporation of waste heat with enough high heat flux into SCPP causes clear enhancement of the power output. With the increase in the EWHF from 0 to 2 GW, the daily total electricity output increases by 119.99% and the PDAR drops from 1.61 to 0.8. Moreover, the PDAR is smaller for weaker solar radiation. This indicates that enough high waste heat flux helps to flatten the daily profile of the plant power output, especially for weak solar radiation. While low waste heat flux has little influence on the power output and the PDAR.
IgG4-assoziierte Erkrankung
Authors: Schirmer Jan H. , Hoyer Bimba F. 
Published: December 2019

Was ist neu?
Übersicht und Nomenklatur Die IgG4-assoziierte Erkrankung (IgG4-RD) ist eine erst kürzlich beschriebene heterogene Krankheitsentität, der viele vorher als eigenständige Einzelorganerkrankungen interpretierte Manifestationen zugerechnet werden. Hauptsymptom ist die Schwellung oder Vergrößerung betroffener Organe.
Diagnose und Klassifikation Die Diagnose wird in Zusammenschau von Anamnese, klinischem Bild, Bildgebung, Histopathologie und IgG4-spezifischen Tests in Labor und Histologie gestellt.
Labor und Bildgebung Häufige Laborbefunde sind: erhöhtes Serum-IgG4, IgE und CRP, Hypergammaglobulinämie, Hypokomplementämie und Eosinophilie.
Histopathologie. Das typische Bild umfasst folgende Hauptcharakteristika: dichte lymphoplasmazelluläre Infiltrate, storiforme Fibrose und obliterative Phlebitis. Ferner treten vermehrte IgG4-positive Plasmazellen auf.
Differenzialdiagnostische Abgrenzung Diverse andere Krankheitsbilder können die IgG4-RD imitieren.
Medikamentöse Therapie Neu sind eine Studie zu Mycophenolat-Mofetil und Daten zu Rituximab bei sonst weitgehend fehlender Evidenz aus Studien zur Behandlung der IgG4-RD.
Glukokortikoide Glukokortikoide (GC) sind die Grundlage der medikamentösen Therapie.
Konventionelle Immunsuppressiva Es herrscht weiterhin kein Konsens, ob konventionelle Immunsuppressiva als GC-sparende Therapie immer oder nur in besonderen Fällen Teil der medikamentösen Therapie sein sollten.
Biologika Rituximab ist eine etablierte Reservetherapie.
Interventionelle und chirurgische Verfahren Mechanische Obstruktionen und Kompressionen können chirurgische und interventionelle Verfahren notwendig machen.
Linkage of Routine Data to Other Data Sources in Germany: A Practical Example Illustrating Challenges and Solutions
Authors: Langner Ingo , Riedel Oliver , Czwikla Jonas , Heinze Franziska , Rothgang Heinz , Zeeb Hajo , Haug Ulrike 
Published: December 2019

ZusammenfassungRoutinedaten haben ein hohes Potenzial für die epidemiologische und Versorgungsforschung, doch fehlen beispielsweise Informationen zu Todesursachen und es mangelt häufig an detaillierten Informationen u. a. zu Lebensstilfaktoren. In Deutschland ist v. a. aufgrund der strengen Datenschutzauflagen eine unmittelbare Ergänzung dieser Informationen durch andere Datenquellen („Linkage“) mit einigen Herausforderungen verbunden. So besteht eine Herausforderung darin, dass die Routinedatennutzer üblicherweise keinen Zugriff auf Personenidentifikatoren besitzen, die für ein Linkage erforderlich sind. Darüber hinaus sollten sensible Informationen (z. B. die Todesursache) nicht an Institutionen übermittelt werden, die selbst über Personenidentifikatoren verfügen. In diesem Artikel veranschaulichen wir diese zentralen Herausforderungen und stellen entsprechende Lösungen anhand eines Praxisbeispiels vor, bei dem die Abrechnungsdaten von gesetzlichen Krankenkassen mit einem epidemiologischen Krebsregister verknüpft werden, um Informationen zur Todesursache zu erhalten. Wir beschreiben die für das Linkage notwendigen Genehmigungsverfahren, den Datenfluss zwischen den beteiligten Institutionen und erläutern die Gründe für den Datenfluss im Hinblick auf die wesentlichen Herausforderungen. Schließlich verallgemeinern wir die Fragen, die bei der Planung eines Linkage-Verfahrens zu klären sind und zeigen mögliche weitere Herausforderungen auf. Mit diesem praktischen Beispiel zeigen wir, dass ein Abgleich zwischen Routinedaten und anderen Datenquellen in Deutschland umsetzbar ist, dabei aber bestimmte Beschränkungen und Hindernisse berücksichtigt werden müssen.
Percutaneous Ultrasound-Guided Core Needle Biopsy: Comparison of 16-Gauge versus 14-Gauge Needle and the Effect of Coaxial Guidance in 1065 Breast Biopsies – A Prospective Randomized Clinical Noninferiority Trial
Authors: Gruber Ines , Oberlechner Ernst , Heck Kerstin , Hoopmann Uta , Böer Bettina , Fugunt Regina , Gall Christian , Hartkopf Andreas D. , Helms Gisela , Hoffmann Sascha M. , Ott Claudia , Röhm Carmen , Stäbler Annette , Wallwiener Diethelm , Brucker Sara Y. , Hahn Markus 
Published: December 2019

Abstract
Purpose Ultrasound-guided core needle biopsy (CNB) is considered the standard assessment to diagnose sonographically visible suspicious breast mass lesions. Based on nonrandomized trials, the current German guidelines recommend at least three cylinders with ≤ 14-gauge needle biopsy. However, no recommendation is made as to how many specimens are needed with a smaller needle size, such as 16-gauge, or if biopsy with coaxial guidance improves diagnostic accuracy and quality. Therefore, in a prospective monocentric unblinded randomized controlled clinical noninferiority trial, the diagnostic accuracy of 16-gauge versus 14-gauge core needle biopsy, with and without coaxial guidance, was evaluated.
Materials and Methods 1065 breast biopsies were included in order to analyze the number of core samples necessary to obtain an appropriate rate of diagnostic quality adequate for histological evaluation, and to achieve high diagnostic accuracy and diagnostic yield. Histological results were verified by surgery or long-term follow-up of at least two years up to five years.
Results In order to obtain an additive diagnostic accuracy of > 99 %, a minimum of two cylinders with 14-gauge biopsy were required. The diagnostic accuracy and the diagnostic quality of 14-gauge biopsy were not affected by the coaxial technique. When performing a 16-gauge biopsy, five cylinders were required to achieve an additive diagnostic accuracy of > 99 %. Without coaxial guidance, 16-gauge CNB required at least three samples, whereas five needle passes with coaxial-guided 16-gauge biopsy were needed.
Conclusion The diagnostic accuracy and quality of ultrasound-guided 16-gauge core needle biopsy were inferior to the 14-gauge needle size, regardless of the use of a coaxial technique.
Vitamin D und Typ-2-Diabetes: Update 2019
Authors: Gröber Uwe , Holick Michael F. 
Published: December 2019

ZusammenfassungVitamin D spielt u. a. im Insulin- und Glukosestoffwechsel eine Rolle. Bereits ältere Studien weisen auf eine Korrelation hin zwischen niedrigem Vitamin-D-Spiegel und dem Risiko für metabolisches Syndrom sowie der Progression vom Prädiabetes zum manifesten Typ-2-Diabetes. Aktuelle Interventionsstudien und Metaanalysen weisen auf eine Assoziation hin zwischen Vitamin-D-Mangel und erhöhtem Risiko für das Auftreten von Insulinresistenz bzw. der Entwicklung eines Typ-2-Diabetes.Die vorliegenden Daten legen nahe, dass Diabetiker hinsichtlich ihres Stoffwechsels bei Vitamin-D-Mangel besonders von einer Supplementierung profitieren. Bei Patienten mit Insulinresistenz sollte der 25(OH)-D-Status labordiagnostisch objektiviert und ggf. supplementiert werden.
Job Satisfaction of U.A.E. Dental Practitioners
Authors: Al-Buainain Fajer S. , Alzarouni Asma A. , Alshamsi Hissa A. , Arab Arwa H. , Bader Fares , Awad Manal 
Published: December 2019

Abstract
Objective The study aims to analyze job satisfaction among registered clinical dentists in the United Arab Emirates (UAE), and also to explore satisfaction with different work environmental factors and relate them to overall job satisfaction.
Materials and Methods A survey modified from the American Dental Association version of job satisfaction published in 2013 to 2014, was given to 197 licensed dentists in Dubai and Sharjah cities in the UAE. The questionnaire included four main sections, in addition to the demographic factors questions. All questions were answered using the 5-point Likert scale. The only exception was the comfortability in the working environment which was answered using a 3-point Likert scale.
Statistical Analysis Categorical data were presented as frequencies and percentages, and data were analyzed using means and standard deviations. Regression analysis was performed with overall job satisfaction as the dependent variable and seven aspects of satisfaction with work and individual characteristics as the independent variables. An α level of 0.05 was used for tests of statistical significance.
Results The overall job satisfaction of dentists working in the UAE is high compared with other countries. Highest satisfaction was related to the relationship with patients, colleagues, and staff. On the other hand, the least satisfaction was linked to the opportunity for part-time work and benefits package. There were no significant differences between male and female participants regarding all work-related factors apart from autonomy. However, private sector dentists had a higher level of satisfaction compared with the public sector in many work-related factors.
Conclusion There are various dimensions that collectively influence the level of overall job satisfaction. Difference existing between the levels of job satisfaction among private and public sector dentists and between male and female dentists need to be addressed to increase the level of job satisfaction among UAE dentists and thus improve all dental care system.
Influence of Different Pigment Incorporation Methods on Color, Dimensional Stability, and Detail Reproduction of Silicones
Authors: Nobrega Adhara Smith , Malavazi Estefania Marrega , Melo Neto Clóvis Lamartine de Moraes , Ervolino Isabela Caroline de Sousa , Bannwart Lisiane Cristina , Bertoz André Pinheiro de Magalhães , Goiato Marcelo Coelho 
Published: December 2019

Abstract
Objective To analyze the influence of three pigment incorporation methods on color change, dimensional stability, and detail reproduction of the MDX4–4210 and A-2186 silicones.
Materials and Methods The A-2186 and MDX4–4210 silicones were used for preparation of samples, with the incorporation of bronze, black and pink pigments, usingconventional, mechanical, and industrial incorporation methods. Samples were submitted to the initial readings of color (n = 10; 22-mm diameter × 2-mm thickness), detail reproduction, and dimensional stability (n = 10; 30-mm diameter × 3-mm thickness). Readings were also taken at the end of 252, 504 and 1,008 hours of aging cycles.
Results Quantitative data were evaluated by ANOVA and Tukey test, with a level of significance of 5%. The mechanical and industrial methods caused smaller color changes of all samples compared with the conventional method (p < 0.05). In most cases, the mechanical and industrial methods caused less samples’ contraction than the conventional method after aging (p < 0.05). The color change values increased progressively in each aging period for all samples (p < 0.05). The contraction values increased progressively in each aging period for all samples (p < 0.05). In the qualitative analysis of detail reproduction, all samples presented full reproduction of the three grooves, with accurate angles, initially and after the aging periods.
Conclusions The industrial and mechanical methods showed the best results for color and dimensional stability. Despite the statistical differences, all pigment incorporation methods generated acceptable dimensional and color changes of the MDX4-4210 and A-2186 silicones, regardless of the pigment and aging. In addition, the detail reproduction was satisfactory after aging periods in all cases of this study, showing the excellent quality of the A-2186 and MDX4–4210 silicones.
Long-Term Outcomes of Digital Nerve Repair Accompanied by Digital Artery Injury in Flexor Zone 2
Authors: Yildiran Gokce , Sutcu Mustafa , Akdag Osman , Tosun Zekeriya 
Published: December 2019

Abstract
Objectives Better healing results of any tissue or area is closely linked with a well-blood supply in reconstructive surgery. Peripheric nerve healing is closely related to blood supply as well. We aimed to assess whether there was any difference between digital nerve healing with and without extrinsic blood supply.
Methods We assessed 48 patients with unilateral digital nerve injury at zone 2. Twenty-four of them had unrepairable arterial injury and other 24 had no arterial injury. The 24 patients in the “unrepaired artery group” (UA) and 24 patients in the “intact artery group” (IA) were compared.
Results Mean follow-up time was 17.7 months. The mean two-point discrimination (2PD) was 5.29 mm in IA group and 5.37 mm in UA group. One neuroma in IA group and two neuromas in UA group were determined. We found no statistically significant difference between these groups in terms of neuroma, 2PD, and cold intolerance. The results of British Medical Research Council sensory recovery clinical scale were comparable for these two groups.
Conclusion Digital nerve healing is related to numerous factors. We hypothesized that blood flow may be one of these factors; however, at this zone digital artery repair is not the foremost determinant for digital nerve healing. Further researches should be done for upper injury levels. Despite this result, we argue not to leave the digital artery without repairment and we propose to repair both artery and nerve to achieve the normal anatomical integrity and to warrant finger blood flow in possible future injuries.
Characterization of the activity, aggregation, and toxicity of heterodimers of WT and ALS-associated mutant Sod1
Authors: Brasil Aline de Araújo , de Carvalho Mariana Dias Castela , Gerhardt Ellen , Queiroz Daniela Dias , Pereira Marcos Dias , Outeiro Tiago Fleming , Eleutherio Elis Cristina Araujo 
Published: December 2019

SignificanceAggregation of the antioxidant enzyme Sod1 represents common factors of both familial (fALS) and sporadic cases of ALS, a fatal neurodegenerative disease. Although many ALS studies have focused on Sod1 homodimers/homomers, the investigation of Sod1 heterodimers/heteromers remains controversial and has mostly been performed with recombinant proteins in vitro, in the absence of a cellular environment. By using living cells, this study sheds light into a critical issue in the context of fALS, the high toxicity of the WT–mutant heteromeric inclusions, especially WT–A4V heteromers which accumulate both in human cells as well as in chronologically aged yeast cells. Besides the aggregation, we proposed that an inefficient heteromer response against oxidative conditions might contribute to fALS-linked mutant hSod1 toxicity.
Development of an autonomous and bifunctional quorum-sensing circuit for metabolic flux control in engineered
Escherichia coli
Authors: Dinh Christina V. , Prather Kristala L. J. 
Published: December 2019

Significance
Efficient microbial synthesis in challenging pathways relies on dynamic regulation of multiple metabolic fluxes to balance several competing goals. To address these situations, we developed an autonomous, pathway-independent, and layered regulation tool. By incorporating parts from 2 different quorum-sensing systems, the layers of our system can be tuned independently to ensure generalizability. Application of the regulation system to overcoming 2 different sets of challenges in the naringenin and salicylic acid pathways resulted in significant improvements in titer, demonstrating that the system is an effective tool for improving pathway production.
Divergent trophic responses of sympatric penguin species to historic anthropogenic exploitation and recent climate change
Authors: McMahon Kelton W. , Michelson Chantel I. , Hart Tom , McCarthy Matthew D. , Patterson William P. , Polito Michael J. 
Published: December 2019

Significance
We reveal species-specific changes in penguin trophic responses to historic shifts in krill availability over the last century by applying new molecular isotope techniques to historic penguin museum specimens. Generalist foraging gentoo penguins, whose population increased 6-fold in the last 40 y, showed adaptive shifts in trophic position in concert with changes in Antarctic krill availability following historic exploitation of marine mammals and recent climate change. In contrast, chinstrap penguins maintained a consistent krill diet despite changes in krill availability and concurrent population declines. These results highlight how responses to shared environmental change can vary substantially among closely related species, supporting ecological niche theory that specialists will be more sensitive to environmental change than their generalist counterparts.