bluuebunny/crossref_metadata_2025_split huggingface dataset:
The endophytic bacteria of oil palm and areca nut are beneficial as antagonist of Ganoderma boninense and potential as plant growth promoter
Authors: Anggita S A , Munif A , Nawangsih A A , Tryono R 
Published: March 2020

Abstract

Ganoderma boninense is the pathogenic fungi causing Basal Stem Rot (BSR) disease on oil palm. This study aimed to obtain potential endophytic bacteria as antagonist as well as plant growth promoting rhizobacteria (PGPR) agents. This study employed three stepwises as follows: 1) isolation and selection of endophytic bacteria in vitro, 2) in vitro antagonism and plant growth promoting assays, and 3) molecular identification of the bacteria. Endophytic bacteria isolated from the root tissues of oil palm and areca nut totally produced 88 isolates. Among those, EG17, EG26, EG113, EG215, AC28, AC112, and AC214 were investigated further because they showed negative result on both hypersensitive and hemolytic assays suggesting that these are not plant pathogen and harmless to mammals. Antagonism assay showed that three isolates with highest growth inhibitions to G. boninense were exhibited by EG26, EG113 and AC112 with 56.80%, 56.51% and 56.11%, respectively. These isolates also significantly enhanced the growth of rice seedlings as a model plant, particularly AC112 that increased root length and plant height up to 30.77% and 39.57%. Molecular identification using 16S rDNA sequencing showed that EG26, EG113 and AC112 were identical to Bacillus subtilis strain VD1, Bacillus velezensis strain Bac57, Bacillus toyonensis strain JCT-23, respectively.
Degradation of chlorpyrifos and BPMC by the bacteria isolated from contaminated shallot farm soil
Authors: Akhdiya A , Wartono  , Sulaeman Eman 
Published: March 2020

Abstract
Accumulation of insecticide residues is harmful to the environment and human living. The research was conducted to explore the chlorpyriphos and BPMC degrading bacteria from contaminated shallot farm soils and to formulate bacterial consortium to be applied as the insecticides bioremediation agent. Among nineteen bacterial isolates, K10 and K14 bacterial isolates could degrade up to 38.3% and 43.3% chlorpyrifos contained in its growth medium in 5 days, respectively. Two bacterial isolates namely B21 dan B17 could degrade BPMC up to 75.9% dan 77% in 5 days of incubation. Bacterial consortium of K10+K14, K10+B21, and B17+B21 isolates could enhance in-vitro degration of chlorpyrifos up to 89.6%, 88.9%, and 88.1% respectively, while its BPMC degradation enhanced up to 75.9%, 70.3%, dan 69.5% respectively. The highest in-vitro degradation was showed by K10+K14 bacterial consortium. It could degrade up to 79.9% for chlorpyrifos, and 71.9% for BPMC. Base on the 16S rDNA sequence analysis, the isolates have similarity 97.7% to A. baumannii, 96.3% to B. toyonensis, 94.4% to uncultured enterobacter sp. clone 150, and 78.08% to uncultured bacterium clonenck09g01c1 for K10, K14, B17, and B21 bacterial isolates, respectively.
Heterosis of seedling traits and their correlation to aphids infestation in chili pepper
Authors: Daryanto A , Hidayat P , Maharijaya A , M Syukur dan 
Published: March 2020

Abstract
Chili pepper is a vegetable plant in a group of horticulture plants. The seedling vigorous and their genetic potency to reduce the number of aphids infestation can encourage chili pepper productivity. The objective of this research was to study heterosis, heterobeltiosis, and correlation of seedling traits to aphids infestation from ten chili hybrid genotypes derived from five chili inbred lines through half diallel crosses. The experiment was conducted using a Randomized Complete Block Design (RCBD) with three replications at the IPB University experiment field and laboratory, Darmaga. Seedling traits and aphids infestation observed at 5 weeks after sowing. Two aphids were infested per plant and evaluated 12 days after infestation. Heterosis effects were predicted based on the average values of their parents whereas heterobeltiosis were predicted based on the average values of the best parents. Hybrid IPB C5 x IPB C4 had the highest heterosis and heterobeltiosis for all seedling traits. Hybrid IPB C3 x IPB C4, IPB C4 x IPB C313 and IPB C20 x IPB C313 had the highest heterosis and heterobeltiosis for reducing number aphids per leaf and aphids per plant. There was no correlation between seedling traits with aphids infestation.
Development of rice mutant tolerance to salinity through combination of gamma rays irradiation and in-vitro selection
Authors: Yunita R , Dewi I S , Lestar E G , Mastur  
Published: March 2020

Abstract
Global warming caused a large rise in global sea level poses many threats, especially to a country like Indonesia. One of the threats that affected rice field located adjacent to coastal area is increase level of soil salinity. Inpari 34 and Inpari 35 varieties tolerant to salinity at seedling phase cannot perform well when planted in salinity prone area where the increase in salinity affected by the up and down of sea surface occurred during the planting season. The aims of this experiment were to obtain putative salinity-tolerant mutant (M1) at vegetative and generative phase from Inpari 34 and Inpari 35 through combination of gamma rays irradiation and in-vitro selection. The study consisted of several activities, namely embryogenic callus induction, mutation induction by gamma-ray irradiation, callus in-vitro selection by NaCl, regeneration of selected callus, and plantlet acclimatization and greenhouse grow-out. In this experiment 62 and 54 putative salinity-tolerant mutant lines obtained from Inpari 34 and Inpari 35, respectively.
Evaluation of morpho-agronomic characterization Apple cucumber: a new variety of melon from Indonesia
Authors: Saputro N W , Hidayat T , Bayfurqon F M , Khamid M B R 
Published: March 2020

AbstractThe Cucurbitaceae has been known to many farmers as a plant that has beneficial for agriculture industries. For decades plants that belong to Cucurbitaceae such as cucumber (Cucumis sativus), watermelon (Citrullus lanatus), and melon (Cucumis melo) has been cultivated to meet consumer needs. In Indonesia, a new variety of melon has been discovered namely apple cucumber. This new variety has blurred the difference between melon and cucumber due to the vegetative resemblance. This research was conducted to clarify the different characters and to study the morpho-agronomic characteristic in qualitative and quantitative-based on IPGRI descriptors. Important and significant diagnostic characters were observed to developed good cultivar in quality and quantity. 16 qualitative and 15 quantitative characters from 120 characters of apple cucumber were observed to determine the specific characters for good cultivar. Principal Analysis Component (PCA) using Python was used to showed discriminative character and also to distinguished apple cucumber to melon and cucumber based on 120 characters of apple cucumber. Morphological differences existing among melon, cucumber, watermelon, and apple cucumber, in particular, are presented.
Melissopalinological characteristic of stingless bee (Trigona/Tetragonula) honey in Lombok, West Nusa Tenggara
Authors: Anggadhania L , Wahyuni N , Rizqiani K D 
Published: March 2020

Abstract
These research objectives were to identify the honey type and the potential plants as food source of stingless bees in Lombok, West Nusa Tenggara. Stingless bees honey samples were collected on September 2016 from four different locations in Lombok which are North Lombok, West Lombok, Central Lombok, and East Lombok. The pollen analysis was performed using Von Der Ohe et al. (2004) methods with modification. The result showed that stingless bees honey samples from four locations did not have predominant pollen which classified as multifloral honey. Meanwhile in the honey samples there identified 27 pollen types belonging to 21 families. The pollen spectrums of honey samples were diverse, with the highest dominancy of Arecaceae (Cocos nucífera) in North and Central Lombok and also Tiliaceae (Type Tílíaceae) in West and East Lombok.
Design and implementation of ecoholistic vertiminaponic with automated control and internet of things based monitoring system for sustainable urban farming
Authors: Sugeru H , Musa P , Miska M E E 
Published: March 2020

Abstract
The urban farming plant system is a type of plant that requires extra monitoring in its maintenance. Internet of Things is a concept whereby an object has ability to transfer data over a network without requiring human-to-human or human-to-computer interaction. In otherwise, optimizing limited spaces in urban areas we can combine several cultivation technique as integrated urban farming consist of plant culture, aquaculture apiary, veterinary, etc. This study aims to create smartly integrated all those cultivations as one cycle system that we called Ecoholistic Vertiminaponic using automated control and monitoring applications on the systems by utilizing the Internet of Things using Android and Cloud smart phones. Applications on Android smart phones are made to be able to know the sensor data that exist on the Cloud and also control the lights, pumps, and fans on the vertiminaponic system through Cloud. Both tasks can be done remotely via an Android smart phone connected to the internet. By using this system, activities that should be done directly can be done remotely via the internet, including in the monitoring and control of plant systems. As the cycle of cultivation system Ecoholistic Vertiminaponic will support urban sustainable farming and its food self-sufficiency.
Major Rice Pest Development After Increasing Rice Planting Index and Acceleration of Planting Time in Lampung Province
Authors: Widiarta I N , Kiswanto  , Rivaie A A 
Published: March 2020

Abstract
Agriculture development in Indonesia has been targeted to attain food resilient by increasing rice production. Lampung has been involved in rice production increasing activity since 2008 up to now. Rice transplanted area in Lampung was increased by distinctive effort by mean of increasing rice planting index and acceleration of planting time to boost rice planting areas as well as production. The objectives of this study were to observe pest before and after; as well as ongoing rice field with and without increasing rice planting index and acceleration of planting time, and to analyze factors involved in the pest attack. The results showed that brown planthopper and rat were main pest problem. Infested area by brown planthopper and rat were negatively correlated with delayed planting but were increased by increasing planting index. The rise of brown planthopper populations was affected by pest management, planting system, integrated crop management implementation, and resistant variety planted. Meanwhile, the increase of rat attack was influenced by increasing of rice planting index, acceleration of planting time, and the dose of urea fertilizer. The brown planthopper and rat threaten sustainability of rice production in which increasing rice planting index and acceleration of planting time were implemented.
The role of indigenous cellulolytic and amylolytic microbes in cassava pulp during the drying process
Authors: Chalisya N , Sunarti T C , Meryandini A 
Published: March 2020

Abstract
Cassava pulp is a solid waste resulted from the processing of cassava into tapioca. Cassava pulp is composed of cellulose and residual starch extract. In addition, cassava pulp contains undigested oligosaccharides. During the cassava pulp drying process, various indigenous microbes grow so that it affects the quality of dried cassava pulp. This study aimed to examine the role of indigenous cellulolytic and amylolytic microbes in cassava pulp during the drying process. Indigenous microbes were isolated from cassava pulp from 0 to 15 days using CMC and starch agar to obtain cellulolytic and amylolytic bacteria. Thirty-two types of cellulolytic bacteria and four types of amylolytic bacteria from cassava pulp were selected based on their cellulolytic and amylolytic index. The dominance of cellulolytic bacteria occurred during the drying process (15 days). The highest cellulolytic index obtained was 1.47 and the amylolytic index was 0.86. Selected isolates that had a high index value were then further tested for growth and cellulase enzyme activity. Cellulase produced by isolates of COC1 bacteria had the highest enzyme activity at the 15th hour, which was 0.06 U / mL. Cassava pulp which underwent a drying process for 15 days had crude fiber and carbohydrates changes.
Characteristics of mannanase enzyme in actinomycetes isolated from Taman Nasional Bukit Duabelas Jambi
Authors: Evan S , Meryandini A , Sunarti T C 
Published: March 2020

Abstract
Indonesia has about 14.03 million hectars of palm oil plantation. Palm kernel cake (PKC) is a byproduct of palm oil processing majority consist of β-mannan. Mannan is a component of hemicellulose which have an economic value. Mannan can be converted to the manooligosaccharides (MOS) using bacterial enzyme. This study aimed to produce MOS from PKC using actinomycetes from Taman Nasional Bukit Duabelas Jambi. The selection was begun with the production of the clear zone on medium containing PKC then enzyme activity was measured. Isolate HJ4-5B had the highest mannanolytic index up to 0.4. The enzyme activity of isolate HJ4-5B increased significantly up to 0.895 U/mL at 7th day of culture incubation. The highest mannanase activity of isolate HJ4-5B occurs at pH 6 and 70 °C with an incubation time of 7th day which reached 1.186 U/mL.
The enhancement of growth, biomass production and lipid productivity of microalgae Choricystis sp. LBB13-AL045 by the addition of hot water extract of its dried biomass
Authors: Praharyawan S , Rahman D Y , Susilaningsih D 
Published: March 2020

Abstract
This study sought to evaluate the influence of hot water extract addition on cell growth, biomass production and lipid productivity during cultivation of microalgae Choricystis sp. LBB13-AL045 under different light intensity. The cellular growth of microalgae in terms of growth rate and produced biomass increased significantly with the addition of hot water extract into the microalgal culture under both low- and high-light intensity (3000 and 30000 lux, respectively), demonstrating that hot water extract of microalgal biomass positively interferes with the metabolism of microalgae and the production of biomass. Thus, microalgal lipid productivity was eventually increased almost three times when hot water extract (7,5 mg/mL) was employed in the microalgal cultivation with high light intensity. Hot water extract was prepared by the autoclaving varied concentration of dried microalgal biomass (2,5-7,5 mg/mL) in water. The cultivation of Choricystis sp. LBB13-AL045 showed the highest net increase of biomass production (975,05 mg) at hot water extract concentration of 7,5 mg/mL and light intensity of 30000 lux. The current investigation demonstrates that hot water extract of Choricystis’s dried biomass accelerate its growth and induce its lipid productivity for its application in biodiesel production.
Replacement of fish meal with the waste of smoked fish processing and snail (Pila ampulacea) flesh meal in the fermented practical diet of walking Catfish (Clarias sp.)
Authors: Nurhayati A P D , Ashuri N M , Setiawan E , Novanti C , Firmansyah Y , Putro B L , Isnansetyo A 
Published: March 2020

Abstract
Waste of smoked fish processing still contains nutritious ingredients for fish feed. Aims of this research were to determine the effect of giving pellets from fish fumigation waste to the growth and proximate levels of fish. Dumbo catfishes that feed by recycled pellets and characterizing fungal content that might contaminate recycled pellets. Catfishes were grouped into five groups, and feed with a variation of smoked fish waste contents in pellets K.0 (Control), K.1 (0% of recycled waste), K.2 (30% of recycled waste), K.3 (60% of recycled waste), and K.4 (90% of recycled waste). Feeding and nourishment were performed for 30 days. Results indicated that K4 yields the highest yield of weight and length for catfishes with a length of 22.28±0.69 cm and weight 31.74±0.93 gr, in addition to the lowest FCR value 0.77.
Characterization of pyrophosphate-dependent phosphofructokinase α-subunit gene from sugarcane showing gene without intron
Authors: Alamsyah F , Widyasari W , Suhandono S 
Published: March 2020

AbstractPyrophosphate-dependent Phosphofructokinase or PFP is an enzyme that regulate sucrose metabolism. It consists of α- and β-subunits, which encoded byPFPαandPFPβgenes, respectively. Sugarcane PFPa has a strong function in glycolysis and has the potency to be engineered to increase sugarcane yield. Hence, the purpose of this work was to isolate, clone and characterize the sugarcanePFPαgene. Total RNA was isolated from leafrolls of TD 91 sugarcane variety. cDNA synthesis followed by DNA amplification ofPFPαgene were performed using degenerate primers. cDNA and DNA fragments were ligated into pGEM-T Easy vector, which were subsequently introduced toE. colicompetent cells.EcoRIwere used to cut the plasmids for sequencing. Finally, homology searching was conducted using BLASTn, and then the nucleotide sequence was translated to a protein sequence using Bioedit. The results showed thatPFPαcDNA fragment was 900 bp in length. The translated PFPa protein showed binding sites for fructose-6-phosphate and fructose-1,6-biphosphate, which are conserved in all family members of PFP.In silicoanalysis of the DNA fragment showed gene without intron. In conclusion, thePFPαgene from sugarcane has been successfully isolated, cloned and characterized.
In vitro spermatogenesis of shark minnow fish (Osteochilus hasselti Valenciennes 1842) as a potential fish reproductive biotechnology
Authors: Umami M , Sistina Y , Wijayanti G E 
Published: March 2020

Abstract

In vitro spermatogenesis has many clinical applications and hopefully with improvement of research findings this technique will solve the applied fisheries era 4.0. This research aimed to evaluate the effects of testosterone and type of serum on the in vitro spermatogenesis of shark-minnow fish (Osteochilus hasselti). In the present research, four concentrations of testosterone (0, 5, 10 and 15 ng/mL) were tested. The types of serum used were autologous serum and Fetal Bovine Serum (FBS). The results showed that the use autologous serum in the culture medium capable of maintaining the consistency of the tissue culture in culture period and maintaining in vitro spermatogenesis of shark-minnow. This study shown that stage-specific of spermatogenic cells needed certain testosterone concentration. The stage of secondary spermatocyte needed testosterone concentration of 5ng/mL i.e. 50.81±9.29% in medium with autologous serum and 36.47±15.49% in medium with FBS, while stage of spermatid and spermatozoa needed testosterone concentration of 10ng/mL i.e. 36.66±19.81% and spermatozoa 43.45±23.44% in autologous serum and the proportion of spermatid i.e. 42.35±9.09% and spermatozoa 35.25±14.0% in medium with FBS. This study proven that in-vitro spermatogenesis, already demonstrated in shark-minnow fish, offered great promises to cope with reproductive issues in the aquaculture and applied fisheries biotechnology.
Mining and characterization of genomic-based microsatellite markers in duku (Lansium domesticum)
Authors: Sari H P , Efendi D , Suwarno W B , Matra D D 
Published: March 2020

Abstract
Duku (Lansium domesticum) has a high diversity. There are no specific markers to assess genetic diversity in Duku. Microsatellite markers can determine genetic diversity more specifically in the cultivar level. This study aimed to isolate and characterize microsatellite sequences from the assembled-genome database. The markers were developed from 455 010 contigs of the velvet-assembled genome using the MISA program. BLASTN program was used for sequence annotation.Characterization of microsatellite markers was analyzed using bioinformatics. The result was found 7 types of repeat type: 63 contigs of mononucleotide with most motif of T, 450 contigs of dinucleotide with most motif of TA, 3094 contigs of trinucleotide with most motif of AAT, 561 contigs of tetranucleotide with most motif of TTAA, 85 contigs of pentanucleotide with most motif of TTTCT, 144 contigs of hexanucleotide with most motif of AAAAAT and 1331 contigs of compound sequence. This study provides information about specific markers in L. domesticum and will contribute to plant breeding development.
Stingless bee Tetragonula laeviceps and T. aff. biroi: geometric morphometry analysis of wing venation variations
Authors: Laksono P , Raffiudin R , Juliandi B 
Published: March 2020

Abstract

Tetragonula laeviceps from Pandeglang West Java is characterized by a thin honey pot. The purpose of this study was to analyze variations of wing venation among stingless bee T. laeviceps, T. laeviceps (thin honey pot) and T. aff. biroi using geometric morphometric analysis. The research was conducted by digitized the landmark at nine homologs venations of stingless bee anterior right-wing venation from 20 individuals of each species. The results show variations on wing venation in the intra- and interspecies of stingless bees based on the relative warp ordination plot. Landmark numbers 4 and number 6 are the characteristics that separate T. laeviceps and T. laeviceps (thin honey pot), and landmark numbers 1, 2, 5, and number 9 are characteristic between T. laeviceps and T. aff. biroi. Based on relative contribution value, landmark number 7 revealed as significant characters in the genus level and landmark number 9 are characteristics in the intraspecies of Tetragonula. The number of landmark variations and bending energy value of intraspecies are less compare to the interspecies of Tetragonula. The unrooted phylogenetic analysis shows the T. laeviceps, T. laeviceps (thin honey pot) and T. aff. biroi are separate in a different cluster for the intra- and interspecies.
A Method for the Measurement of Thickness of Inorganic Solid Films by Medium Energy Electron Excited X-ray Fluorescence
Authors: Zhou Hailong , Yu Feng , Ji Zhenguo 
Published: March 2020

Abstract
In this paper, a new non-destructive method of measuring the thickness of nanometer thin films by medium energy electron excited X-ray fluorescence was introduced. Due to strong decay of the intensity of incident electron beam with energy of the order of 1 keV in solid materials, the penetration depth is very small, only in the order of 1 nm. Therefore, the escape depth of the X-ray fluorescence excited by the electron beam is also limited by the penetration depth of the incident electron beam, i.e., the X-ray fluorescence detected is emitted only from the top most layers. By simply compare the intensity of an XRF peak from the substrate with/without the deposition of thin film, the thickness of the films can be easily calculated. The method described in this paper is simple in participle, nondestructive, and applicable for films of any elements. As an example, a set of Al films deposited on Si substrate was demonstrated, the results show that this method is feasible, and the detection limit can be as low as 1 nm or less.
Synthesis of a Novel Water-Based Drilling Fluids Loss Reducer
Authors: Du Weichao , Wang Xiangyun , Zhang Yunyue , Chen Gang 
Published: March 2020

Abstract
In this paper, an inorganic/organic water-based drilling fluids reducer AM/AA/GO was prepared by using acrylamide (AM), acrylic acid (AA) and graphene oxide (GO) as raw material. Structural of AM/AA/GO was characterized via FT-IR, GPC and TGA-DSC. GPC results showed that the molecular weight of AM/AA/GO was 23503 g/mol and the TGA-DSC confirmed the excellent temperature resistance ability of AM/AA/GO.