bluuebunny/crossref_metadata_2025_split huggingface dataset:
A trimeric metazoan Rab7 GEF complex is crucial for endocytosis and scavenger function
Authors: Dehnen Lena , Janz Maren , Verma Jitender Kumar , Psathaki Olympia Ekaterini , Langemeyer Lars , Fröhlich Florian , Heinisch Jürgen J. , Meyer Heiko , Ungermann Christian , Paululat Achim 
Published: June 2020

ABSTRACT
Endosome biogenesis in eukaryotic cells is critical for nutrient uptake and plasma membrane integrity. Early endosomes initially contain Rab5, which is replaced by Rab7 on late endosomes prior to their fusion with lysosomes. Recruitment of Rab7 to endosomes requires the Mon1–Ccz1 guanine-nucleotide-exchange factor (GEF). Here, we show that full function of the Drosophila Mon1–Ccz1 complex requires a third stoichiometric subunit, termed Bulli (encoded by CG8270). Bulli localises to Rab7-positive endosomes, in agreement with its function in the GEF complex. Using Drosophila nephrocytes as a model system, we observe that absence of Bulli results in (i) reduced endocytosis, (ii) Rab5 accumulation within non-acidified enlarged endosomes, (iii) defective Rab7 localisation and (iv) impaired endosomal maturation. Moreover, longevity of animals lacking bulli is affected. Both the Mon1–Ccz1 dimer and a Bulli-containing trimer display Rab7 GEF activity. In summary, this suggests a key role for Bulli in the Rab5 to Rab7 transition during endosomal maturation rather than a direct influence on the GEF activity of Mon1–Ccz1.
Functionalization of Primary C–H Bonds in Picolines toward Pyridylthioamides
Authors: Ho Tuan H , Le Ha H K , To Tuong A , Nguyen Tung T , Phan Nam T S 
Published: June 2020

Abstract
We report a method for coupling of nitroarenes, 2- or 4-methylazaarenes, and elemental sulfur to afford (2-pyridyl)aryl thioamides. Good tolerance of functionalities was observed, including primary and secondary amines, bromo, iodo, ester, and boronic ester groups. Thioamides derived from pyrazole, thiazole, quinoline, quinoxalines, and azoles were obtained in good yields, showing the compatibility of heterocycles. This appears to be a general method to obtain pyridyl thioamides in one step from commercial, simple substrates.
Asymmetric Synthesis of 1-Tetralones Bearing a Remote Quaternary Stereocenter through Rh-Catalyzed C–C Activation of Cyclopentanones
Authors: Ochi Shusuke , Xia Ying , Dong Guangbin 
Published: June 2020

Abstract
Herein, we describe the preparation of 1-tetralones bearing a remote quaternary stereocenter in a highly enantioselective manner. A sequence of Pd-catalyzed asymmetric 1,4-addition and Rh-catalyzed enantiospecific C–C/C–H activation delivers diverse 1-tetralones with a C4 quaternary stereocenter, which are prepared in good overall yields and high enantioselectivity.
Computational screening for potential drug candidates against the SARS-CoV-2 main protease
Authors: Silva Andrade Bruno , Ghosh Preetam , Barh Debmalya , Tiwari Sandeep , José Santana Silva Raner , Rodrigues de Assis Soares Wagner , Silva Melo Tarcisio , Santos Freitas Andria , González-Grande Patrícia , Sousa Palmeira Lucas , Carlos Junior Alcantara Luiz , Giovanetti Marta , Góes-Neto Aristóteles , Ariston de Carvalho Azevedo Vasco 
Published: June 2020

Background: SARS-CoV-2 is the causal agent of the current coronavirus disease 2019 (COVID-19) pandemic. They are enveloped, positive-sense, single-stranded RNA viruses of the Coronaviridae family. Proteases of SARS-CoV-2 are necessary for viral replication, structural assembly, and pathogenicity. The approximately 33.8 kDa Mpro protease of SARS-CoV-2 is a non-human homologue and is highly conserved among several coronaviruses, indicating that Mpro could be a potential drug target for Coronaviruses. Methods: Herein, we performed computational ligand screening of four pharmacophores (OEW, remdesivir, hydroxychloroquine and N3) that are presumed to have positive effects against SARS-CoV-2 Mpro protease (6LU7), and also screened 50,000 natural compounds from the ZINC Database dataset against this protease target. Results: We found 40 pharmacophore-like structures of natural compounds from diverse chemical classes that exhibited better affinity of docking as compared to the known ligands. The 11 best selected ligands, namely ZINC1845382, ZINC1875405, ZINC2092396, ZINC2104424, ZINC44018332, ZINC2101723, ZINC2094526, ZINC2094304, ZINC2104482, ZINC3984030, and ZINC1531664, are mainly classified as beta-carboline, alkaloids, and polyflavonoids, and all displayed interactions with dyad CYS145 and HIS41 from the protease pocket in a similar way as other known ligands. Conclusions: Our results suggest that these 11 molecules could be effective against SARS-CoV-2 protease and may be subsequently tested in vitro and in vivo to develop novel drugs against this virus.
Rectal gonorrhoea and chlamydia among men who have sex with men in coastal Kenya
Authors: Ngetsa Caroline J. , Heymann Marc W. , Thiong'o Alex , Wahome Elizabeth , Mwambi John , Karani Clara , Menza Nelson C. , Mwashigadi Grace , Muturi Margaret W. , Graham Susan M. , Mugo Peter M. , Sanders Eduard J. 
Published: June 2020

Background: Men who have sex with men (MSM) have a higher prevalence of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) infections compared to the rest of the population, often remaining undiagnosed. In Kenya, prevalence of rectal CT and NG infection and NG antimicrobial sensitivity are poorly described. Methods: MSM who reported receptive anal intercourse (RAI) were recruited from an ongoing human immunodeficiency virus acquisition and treatment study in coastal Kenya in 2016-2017. Rectal swabs were collected at two time points 6 months apart to estimate prevalence and incidence of CT/NG infection using a molecular point-of-care assay. Participants positive for CT or NG were treated according to national guidelines. NG culture and antimicrobial susceptibility testing was performed. Participant and risk behaviour characteristics were collected and association with baseline CT/NG prevalence assessed by multivariable regression analysis. Results: Prevalence of CT/NG in 104 MSM was 21.2% (CT 13.5%, NG 9.6%, dual infection 1.9%) at baseline and 25.9% in 81 MSM at follow-up (CT 14.8%, NG 14.8%, dual infection 3.7%). CT/NG incidence was estimated at 53.0 (95% CI, 34.5-81.3) per 100 person-years. Most CT/NG positive participants were asymptomatic: 95.5% at baseline and 100% at follow-up. CT/NG infection was associated with being paid for sex [adjusted odds ratio (aOR)=6.2, 95% CI (1.7-22.9)] and being in formal employment [aOR=7.5, 95% CI (1.1-49.2)]. Six NG isolates were obtained at follow-up; all were susceptible to ceftriaxone and cefixime and all were resistant to penicillin, tetracycline and ciprofloxacin. Conclusions: There is a high prevalence and incidence of asymptomatic rectal CT and NG in MSM reporting RAI in coastal Kenya. MSM who were paid for sex or had formal employment were more likely to be infected with CT/NG suggesting increased risk behaviour during transactional sex. Antimicrobial susceptibility results suggest that current antibiotic choices in Kenya are appropriate for NG treatment.
The Pediatric Hospital Medicine Core Competencies: 2020 Revision Introduction and Methodology
Authors: Maniscalco Jennifer , Gage Sandra , Teferi Sofa , Fisher Erin Stucky 
Published: June 2020

BACKGROUNDThe Pediatric Hospital Medicine (PHM) Core Competencies define the expertise required of practitioners and provide a framework for educational activities. Since initial publication in 2010, the scope of practice for pediatric hospitalists has evolved in clinical, research, administrative, and educational arenas.OBJECTIVETo describe the methodology utilized in the revision of The PHM Core Competencies to ensure a product reflective of current roles and expectations for pediatric hospitalists across all training pathways and practice settings.METHODSThe Society of Hospital Medicine (SHM) Pediatrics Special Interest Group supported the initiation of the revision. A diverse group of editors and authors was engaged from among members of SHM, the American Academy of Pediatrics and the Academic Pediatrics Association. Editorial roles were expanded to represent all practice settings. More than 80 individuals contributed, representing both university and community sites, and all US geographic regions. Editors conducted a two‐part needs assessment; a survey related to content was distributed to the PHM community and content from recent conferences and PHM related publications was reviewed. The final compendium consists of 4 sections and 66 chapters, including 12 new chapters and 36 chapters with substantial changes. Individual chapters and the entire compendium underwent rigorous internal and external review.CONCLUSIONThe PHM Core Competencies: 2020 Revision reflects the work of a broad spectrum of PHM practitioners responding to the practice and educational changes in PHM over the past decade. The compendium can inform education, training, and career development for pediatric hospitalists practicing now and in coming years.
Portable quantitative phase microscope for material metrology and biological imaging
Authors: Niu Mengxuan , Luo Gang , Shu Xin , Qu Fuyang , Zhou Shuang , Ho Yi-Ping , Zhao Ni , Zhou Renjie 
Published: June 2020

Quantitative phase microscopy (QPM) has emerged as an important tool for material metrology and biological imaging. For broader adoption in those applications, we have proposed and demonstrated a new portable off-axis QPM method, which works in both transmission and reflection modes to meet different sample measurement requirements. The temporal and spatial sensitivities of our system, as quantified by optical path-length difference values, are 0.65 nm and 1.04 nm, respectively. To demonstrate its applicability for a wide range of applications, we deployed our system for profiling transistor gold electrode samples, observing red blood cell membrane fluctuations, imaging living cells flowing in a microfluidic chip, etc. Our portable QPM system has a low-cost design and involves a simple and robust phase-retrieval algorithm that we envision will allow for broader deployment at different environmental settings, including in resource-limited sites and integration with other metrology or imaging modalities.
The Sage Handbook of Media and Migration, Kevin Smets, Koen Leurs, Myria Georgiou, Saskia Witteborn and Radhika Gajjala (eds) (2019)
Authors: Gabor Elena 
Published: June 2020

Review of: The Sage Handbook of Media and Migration, Kevin Smets, Koen Leurs, Myria Georgiou, Saskia Witteborn and Radhika Gajjala (eds) (2019)
Thousand Oaks, CA: Sage, 684 pp.,
ISBN 978-1-52644-721-0, e-book, $93
Gastroprotective effect of ethylacetate fraction of the leaves of Hannoa klaineana on aspirin and histamine-induced gastric ulcer in rats
Authors: Abubakar Ibrahim , Muhammad Yankuzo Hassan , Shuaibu Baraya Yusha'u , Abubakar Gusau Mu'azu 
Published: June 2020

Background: Peptic ulcer disease remains endemic in our society affecting about four million people every year worldwide. Hannoa klaineana is used traditionally in the treatment of various gastrointestinal diseases including ulcer.Aim: This study aims at evaluating the gastroprotective effect of ethylacetate fraction of the leaves of Hannoa klaineana (Simaroubaceae).Methods: The gastroprotective effect of ethylacetate fraction of the Hannoa klaineana (50, 100 and 200mg/kg b.wt) was evaluated using aspirin and histamine induced ulcer models.Results: In aspirin-induced ulcer model, the ethylacetate fraction of the Hannoa klaineana demonstrated significant (p<0.001) decreased in mean ulcer index with the maximum protective effect (99.84%) at 200 mg/kg against the gastric damages. While histamine-induced ulcer model, the solvent fraction significantly (p<0.001) decreased mean ulcer index with the protective effect up to 99.83% against the gastric lesions. In both models, a significant (p<0.001) increased in pH value coupled with significant (p<0.001) decreased in gastric volume, free and total acidity in rats pre-treated with varying doses of the ethylacetate fraction was found.Conclusion: The mechanism of gastroprotective effects of ethylacetate fraction of the Hannoa klaineana could be attributed to its ability to stimulate prostaglandins secretion or possess prostaglandins like-substances or suppression of histamine-induced vasospastic effect and gastric secretion.Â Â Â
Marco de desarrollo algorítmico de inteligencia de enjambres aplicada en almacenes
Authors: González Pinzón Cesar Leonardo , Espitia Cuchango Helbert Eduardo , Avendaño Prieto Gerardo 
Published: June 2020

La inteligencia de enjambres biológicos ha tenido un alto desarrollo en diferentes campos de la ingeniería aplicada, como es el caso de la robótica colaborativa en donde, a través de la bioinspiración, se desarrollan algoritmos que buscan imitar los comportamientos emergentes que suceden en la naturaleza cuando interactúan los integrantes de un enjambre de manera local, generando una inteligencia para resolver una problemática de manera auto organizada. Dentro de las aplicaciones en campos de la ingeniera está el desarrollo de procesos automatizados en ambientes de toda la cadena productiva de las empresas. Es por ello que este articulo busca dar un marco de referencia en la automatización de almacenes de picking, aplicando algoritmos de inteligencia artificial, específicamente la técnica de inteligencia de enjambres, que posibilite el uso de agentes que interactúen de forma colaborativa (cero colisiones, manipulación de objetos, entre otras) y competitiva (menor gasto de energía para el desarrollo de actividades), bajo un esquema de comunicación ambiente – agente, agente.
Design and simulation of a non-resonant antenna: waveguide slot antenna for 30 GHz
Authors: Montiel Óscar I , Ayala Mario A. , Ripoll S. Lácides 
Published: June 2020

The main contribution of this article is the design and simulation of a non-resonant slot antenna based on wave guides to operate in the range of microwaves. The design is done using MATLAB and the Chebyshev synthesis to determine the distribution of current in each element. Based on this information, the size and distribution of the elements are calculated. Different design conditions are simulated, such as: changes in the level from main lobe to secondary and dierent number of slots at one frequency. The slots are interleaved with respect to the axis of the waveguide and separated by a distance (d). To determine its dimensions, it is necessary to deduce the conductances of the array. A particular design case is compared with simulations from the software CST Microwave Studio. It is observed that radiation diagrams are very similar in both number and amplitude of the lobes, validating the tool used.
Problema de asignación quadrática (pac) sobre gpu a través de una pga maestro-esclavo
Authors: Castellanos Millán Julián Octavio , Amarillo Calvo Víctor Hugo , Poveda Chaves Roberto Manuel 
Published: June 2020

Este documento describe la implementación de un algoritmo genético paralelo maestroesclavo (AGP) en unidades de procesamiento gráfico (UPG) para encontrar soluciones o soluciones cercanas a soluciones óptimas para casos particulares del Problema de asignación Cuadrática (PAC). La eficiencia del algoritmo se prueba en un conjunto de problemas de la biblioteca estándar QAPLIB.
Development of a grip and support system for public transport
Authors: Conto-Campis Juan Camilo , Ortiz-Guzmán Jessica Marlen , Castiblanco-Jiménez Ivonne Angélica , Ortiz-Guzmán Johan Enrique 
Published: June 2020

It is given that in Bogotá public transport system moves approximately 29.18% of the population, it is essential to ensure that the features of the service provision are the most appropriate for its users; for example, the support bars inside the vehicle are not at an adequate height according to the physical characteristics of the users, generating risks within the vehicles. Due to this failure has not been resolved in its entirety by the service provider is important to implement a method that allows adequate grip to the fasteners, considering this, the project aims to develop a system of grip and support that reduces the risks of physical damage to the user during his trip. This system will allow users who are in the orthostatic position (standing) to anchor themselves in a simple way of the elements incorporated in the vehicle. The development process was carried out using a combination of the Ulrich and Schnarch methodologies
Bridging two worlds with RICE
Authors: Groß Philipp , Lehner Wolfgang , Weichert Thomas , Färber Franz , Li Wen-Syan 
Published: June 2020

The growing need to use large amounts of data as the basis for sophisticated business analysis conflicts with the current capabilities of statistical software systems as well as the functions provided by most modern databases.
We developed two novel approaches towards a solution for this basic conflict, based on the widely-used statistical software package R and the SAP In-Memory Computing Engine (IMCE).
We thereby propose an alternative data exchange mechanism with R. Instead of using standard SQL interfaces like JDBC or ODBC we introduced SQL-SHM, a shared memory-based data exchange to incorporate R's vertical data structure. Furthermore, we extended this approach to R-Op introducing R scripts equivalent to native database operations like join or aggregation within the execution plans.
With the calculation engine, IMCE provides a framework to model logical execution plans and thereby offers a convenient way to use the full functionality of R via SQL interface. Moreover, this enables us to run R scripts in parallel without the necessity of extending the R interpreter itself.
Citrusleaf
Authors: Srinivasan V. , Bulkowski Brian 
Published: June 2020

In this paper, we describe the Citrusleaf real-time distributed database platform that is built using the core principles of traditional database consistency and reliability while also being fast and flexible enough for use in high-performance applications like real-time bidding. In fact, Citrusleaf is unique among NoSQL databases for its ability to provide immediate consistency and ACID while still being able to consistently exceed the high performance and scalability standards required by demanding real-time applications. This paper describes how the Citrusleaf system achieves the marriage of traditional database reliability, including immediate consistency and ACID, with flexibility and operational efficiency.
Citrusleaf scales linearly at extremely high throughput while keeping response time in the sub-millisecond range as demonstrated by the test results presented here. This kind of performance has enabled Citrusleaf to become the underlying component of some of the world's largest real-time bidding networks.
Formation of laser-induced periodic surface structures on different materials: fundamentals, properties and applications
Authors: Gräf Stephan 
Published: June 2020

Abstract
The use of ultra-short pulsed lasers enables the fabrication of laser-induced periodic surface structures (LIPSS) on various materials following a single-step, direct-writing technique. These specific, well-ordered nanostructures with periodicities in the order of the utilised laser wavelength facilitate the engineering of surfaces with functional properties. This review paper discusses the physical background of LIPSS formation on substrates with different material properties. Using the examples of structural colours, specific wetting states and the reduction of friction and wear, this work presents experimental approaches that allow to deliberately influence the LIPSS formation process and thus tailor the surface properties. Finally, the review concludes with some future developments and perspectives related to forthcoming applications of LIPSS-based surfaces are discussed.
Ultrafast laser micro-nano structured superhydrophobic teflon surfaces for enhanced SERS detection via evaporation concentration
Authors: Hu Xinyu , Pan Rui , Cai Mingyong , Liu Weijian , Luo Xiao , Chen Changhao , Jiang Guochen , Zhong Minlin 
Published: June 2020

Abstract
Evaporation concentration of target analytes dissolved in a water droplet based on superhydrophobic surfaces could be able to break the limits for sensitive trace substance detection techniques (e.g. SERS) and it is promising in the fields such as food safety, eco-pollution, and bioscience. In the present study, polytetrafluoroethylene (PTFE) surfaces were processed by femtosecond laser and the corresponding processing parameter combinations were optimised to obtain surfaces with excellent superhydrophobicity. The optimal parameter combination is: laser power: 6.4 W; scanning spacing: 40 μm; scanning number: 1; and scanning path: 90 degree. For trapping and localising droplets, a tiny square area in the middle of the surface remained unprocessed for each sample. The evaporation and concentration processes of droplets on the optimised surfaces were performed and analyzed, respectively. It is shown that the droplets with targeted solute can successfully collect all solute into the designed trapping areas during evaporation process on our laser fabricated superhydrophobic surface, resulting in detection domains with high solute concentration for SERS characterisation. It is shown that the detected peak intensity of rhodamine 6G with a concentration of 10−6
m in SERS characterisation can be obviously enhanced by one or two orders of magnitude on the laser fabricated surfaces compared with that of the unprocessed blank samples.
Laboratory practices to mitigate biohazard risks during the COVID-19 outbreak: an IFCC global survey
Authors: Loh Tze Ping , Horvath Andrea Rita , Wang Cheng-Bin , Koch David , Lippi Giuseppe , Mancini Nicasio , Ferrari Maurizio , Hawkins Robert , Sethi Sunil , Adeli Khosrow 
Published: June 2020

Abstract

Objectives
A global survey was conducted by the IFCC Task Force on COVID-19 to better understand how general biochemistry laboratories manage the pre-analytical, analytical and post-analytical processes to mitigate biohazard risks during the coronavirus disease 2019 (COVID-19) pandemic.

Methods
An electronic survey was developed to record the general characteristics of the laboratory, as well as the pre-analytical, analytical, post-analytical and operational practices of biochemistry laboratories that are managing clinical samples of patients with COVID-19.

Results
A total of 1210 submissions were included in the analysis. The majority of responses came from hospital central/core laboratories that serve hospital patient groups and handle moderate daily sample volumes. There has been a decrease in the use of pneumatic tube transport, increase in hand delivery and increase in number of layers of plastic bags for samples of patients with clinically suspected or confirmed COVID-19. Surgical face masks and gloves are the most commonly used personal protective equipment (PPE). Just >50% of the laboratories did not perform an additional decontamination step on the instrument after analysis of samples from patients with clinically suspected or confirmed COVID-19. A fifth of laboratories disallowed add-on testing on these samples. Less than a quarter of laboratories autoclaved their samples prior to disposal.

Conclusions
The survey responses showed wide variation in pre-analytical, analytical and post-analytical practices in terms of PPE adoption and biosafety processes. It is likely that many of the suboptimal biosafety practices are related to practical local factors, such as limited PPE availability and lack of automated instrumentation.