bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of hsa_circ_005243 induces trophoblast cell dysfunction and inflammation via the β-catenin and NF-κB pathways in gestational diabetes mellitus
Authors: Wang Huiyan , Zhou Wenbo , She Guangtong , Yu Bin , Sun Lizhou 
Published: March 2020

Abstract
Background: Gestational diabetes mellitus (GDM) is a common complication in pregnancy that poses a serious threat to the health of both mother and child. While the specific etiology and pathogenesis of this disease are not fully understood, it is thought to arise due to a combination of insulin resistance, inflammation, and genetic factors. Circular RNAs (circRNAs) are a special kind of non-coding RNA that have attracted significant attention in recent years due to their diverse activities, including a potential regulatory role in pregnancy-related diseases, such as GDM. Methods: We previously reported the existence of a novel a circRNA, hsa_circ_005243, which was identified by RNA sequencing. In this study, we examined its expression in 20 pregnant women with GDM and 20 normal controls using quantitative reverse transcription PCR analysis. Subsequent in vitro experiments were conducted following hsa_circ_005243 knockdown in HTR-8/SVneo cells to examine the role of hsa_circ_005243 in cell proliferation and migration, as well as the secretion of inflammatory factors such as tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6). Finally, we examined the expression of β-catenin and nuclear factor kappa-B (NF-κB) signaling pathways to assess their role in GDM pathogenesis.Results: Expression of hsa_circ_005243 was significantly reduced in both the placenta and plasma of GDM patients. Knockdown of hsa_circ_005243 in trophoblast cells significantly suppressed cell proliferation and migration ability. In addition, increased secretion of inflammatory factors (TNF-α and IL-6) was observed after hsa_circ_005243 depletion. Further analyses showed that knockdown of hsa_circ_005243 reduced the expression of β-catenin and increased nuclear NF-κB p65 nuclear translocation. Conclusions: Downregulation of hsa_circ_005243 may be associated with the pathogenesis of GDM via the regulation of β-catenin and NF-κB signal pathways, suggesting a new potential therapeutic target for GDM.
Risk factors associated with canine overweightness and obesity in an owner-reported survey
Authors: Perry LeeAnn , Shmalberg Justin William , Tanprasertsuk Jirayu , Massey Dan , Honaker Ryan William , Jha Aashish R 
Published: March 2020

Abstract
Background

Overweightness and obesity in dogs are associated with negative health outcomes. A better understanding of risk factors associated with canine weight is fundamental to identifying preventative interventions and treatments. In this cross-sectional study, we used a direct to consumer approach to collect body condition scores (BCS), as well as demographic, diet, and lifestyle data on 4,446 dogs. BCS was assessed by owners using a 9-point system and categorized as ideal (BCS 4-5), overweight (BCS 6), and obese (BCS 7+). Following univariate analyses, a stepwise procedure was used to select variables which were included in multivariate logistic regression models. One model was created to compare ideal to all overweight and obese dogs, and another was created to compare ideal to obese dogs only. We then used Elastic Net selection and XGBoost variable importance measures to validate these results.

Results

Overall, 1,480 (33%) of dogs were reported to be overweight or obese, of which 356 (8% total) of dogs were reported to be obese. Seven factors were significantly associated with both overweightness/obesity and obesity alone in all three analyses (stepwise, Elastic Net, and XGBoost): diet composition, probiotic supplementation, treat quantity, exercise, age, food motivation level, and pet appetite. Neutering was also associated with overweightness/obesity in all analyses.

Conclusions

This study recapitulated established risk factors associated with BCS (age, exercise, neutering). Moreover, we elucidated associations between previously examined risk factors and BCS (diet composition, treat consumption, and temperament) and identified a novel factor (probiotic supplementation). Specifically, relative to dogs on fresh food diets, BCS was higher in dogs eating dry food both alone and in combination with other foods. Furthermore, dogs receiving probiotics, but not other forms of supplementation, were more likely to have an ideal BCS. Future studies should corroborate these findings with experimental manipulations.
Effects of parathyroid hormone and vitamin D supplementation on stroke among patients receiving peritoneal dialysis
Authors: You Xiaohan , Zhou Ying , Zhang Jianna , Zhou Qiongxiu , Shi Yanling , Su Zhen , Chen Chaoshen , Shao Rongrong , Zhang Ji 
Published: March 2020

Abstract
Background: Continuous ambulatory peritoneal dialysis (CAPD) patients have a high incidence of stroke and commonly have increased parathyroid hormone levels and vitamin D insufficiency. We seek to investigate the incidence of stroke and the role of parathyroid hormone and vitamin D supplementation in stroke risk among CAPD patients.Methods: This study employed a retrospective design. We enrolled a Chinese cohort of 980 CAPD patients who were routinely followed in our department. The demographic and clinical data were recorded at the time of initial CAPD and during follow-up. The included patients were separated into non-stroke and stroke groups. The effects of parathyroid hormone and vitamin D supplementation on stroke in CAPD patients was evaluated. The primary endpoint is defined as the first occurrence of stroke, and composite endpoint events are defined as death or switch to hemodialysis during follow-up.Results: A total of 757 eligible CAPD patients with a mean follow-up time of 54.7 (standard deviation, 33) months were included in the study. The median incidence of stroke among our CAPD patients was 18.9 (interquartile range, 15.7 - 22.1) per 1000 person-years. A significant nonlinear correlation between baseline iPTH and hazard of stroke (p-value of linear association = 0.2 and nonlinear association = 0.002) was observed in our univariate Cox regression analysis, and low baseline iPTH levels (≤150 pg/ml) were associated with an increased cumulative hazard of stroke. Multivariate Cox regression analysis indicated a significant interaction effect between age and iPTH after adjusting for other confounders. Vitamin D supplementation during follow-up was a predictive factor for stroke in our cohort.Conclusions: CAPD patients suffered a high risk of stroke, and lower iPTH levels were significantly correlated with an increased risk of stroke. Nevertheless, vitamin D supplementation may reduce the risk of stroke in these patients.
Primary carnitine deficiency – diagnosis after heart transplantation: Better late than never!
Authors: Grünert Sarah Catharina , Tucci Sara , Schumann Anke , Schwendt Meike , Gramer Gwendolyn , Hoffmann Georg F. , Erbel Michelle , Stiller Brigitte , Spiekerkoetter Ute 
Published: March 2020

Abstract
Background Primary carnitine deficiency due to mutations in the OCTN2 gene is a rare but well-treatable metabolic disorder that puts patients at risk for metabolic decompensations, skeletal and cardiac myopathy and sudden cardiac death. Results We report on a 7-year-old boy diagnosed with primary carnitine deficiency 2 years after successful heart transplantation thanks his younger sister’s having been identified via expanded newborn screening during a pilot study evaluating an extension of the German newborn screening panel. Conclusion As L-carnitine supplementation can prevent and mostly reverse clinical symptoms of primary carnitine deficiency, all patients with cardiomyopathy should be investigated for primary carnitine deficiency even if newborn screening results were unremarkable.
Very low levels of serum anti- Müllerian hormone as a possible marker for follicle growth in patients with primary ovarian insufficiency under hormone replacement therapy
Authors: Kasahara Yukiyo , Osuka Satoko , Bayasula Bayasula , Nakanishi Natsuki , Murase Tomohiko , Nakamura Tomoko , Goto Maki , Kotani Tomomi , Iwase Akira , Kikkawa Fumitaka 
Published: March 2020

Abstract
Background

Patients with primary ovarian insufficiency (POI) occasionally present with follicle growth; however, it is difficult to predict exactly which cycles will show follicle growth. Serum anti-Müllerian hormone (AMH), a useful marker for ovarian reserve, is produced in early-stage follicles. Therefore, AMH levels should reflect the presence of small follicles, which are difficult to detect using ultrasonography, and may be useful as a marker for predicting follicle growth in patients with POI.

Methods

Using an ultrasensitive enzyme-linked immunosorbent assay (ELISA) kit, we found very low serum AMH levels in patients with POI. We assessed the follicle growth of each patient during each cycle to clarify the usefulness of measuring serum AMH levels for predicting follicle growth in patients with POI under hormone replacement therapy (HRT).

Results

A total of 91 cycles of 19 patients with POI were analyzed in this study. Five patients presented with positive serum AMH levels during the observational periods, and all five experienced follicle growth. Only two of the 14 patients with negative serum AMH levels had follicle growth. Serum AMH and follicle-stimulating hormone (FSH) levels in cycles with follicle growth were significantly higher than in cycles without follicle growth. The median serum AMH level (2.77 pg/mL; 25 th , 75 th percentiles: 0.0, 9.64) in cycles with follicle growth was lower than the lower limit of detection of conventional AMH ELISA kits. The positive predictive value of positive serum AMH levels for follicle growth was higher than that of FSH (< 10 mIU/mL).

Conclusions

Measurement of very low levels of serum AMH using picoAMH assays is useful for prediction of follicle growth in patients with POI under HRT conditions.
Preoperative Prostate Health Index predicts adverse pathology and Gleason score upgrading after radical prostatectomy for prostate cancer
Authors: Novák Vojtěch , Veselý Štěpán , Lukšanová Hana , Průša Richard , Čapoun Otakar , Fiala Vojtěch , Dolejšová Olga , Sedláčková Hana , Kučera Radek , Stejskal Jiří , Záleský Miroslav , Babjuk Marko 
Published: March 2020

Abstract
Background: We aimed to explore the utility of prostate specific antigen (PSA) isoform [-2]proPSA and its derivatives for prediction of pathological outcome after radical prostatectomy (RP).Methods: Preoperative blood samples were prospectively and consecutively analyzed from 472 patients treated with RP for clinically localized prostate cancer at four medical centers. Measured parameters were PSA, free PSA (fPSA), fPSA/PSA ratio, [-2]proPSA (p2PSA), p2PSA/fPSA ratio and Prostate Health Index (PHI) (p2PSA/fPSA)*√PSA]. Logistic regression models were fitted to determine the accuracy of markers for prediction of pathological Gleason score (GS) ≥7, Gleason score upgrading, extracapsular extension of the tumor (pT3) and the presence of positive surgical margin (PSM). Results: Of 472 patients undergoing RP, 339 (72%) were found to have pathologic GS ≥ 7, out of them 178 (53%) experienced an upgrade from their preoperative GS=6. The findings of pT3 and PSM were present in 132 (28%) and 133 (28%) cases, respectively. At univariable analysis of all the preoperative parameters, PHI was the most accurate predictor of pathological GS ≥7, GS upgrading, pT3 disease and the presence of PSM. Adding of PHI into the base multivariable model increased significantly the accuracy for prediction of pathological GS and GS upgrading by 4.4% (p=0.015) and 5.0% (p=0.025), respectively. Conclusion: We found that PHI provides the highest accuracy in predicting prostate cancer aggressiveness and expansion of the tumor detected at final pathology. The ability of PHI to predict the risk of Gleason score upgrade may help to identify potentially high-risk patients among men with biopsy proven insignificant prostate cancer.
Hemodynamic changes in regular antipsychotic users after adrenaline-containing lidocaine injection under general anesthesia: a prospective study
Authors: Shionoya Yoshiki , Nakamura Eishi , Tsujimoto Gentaro , Koyata Takayuki , Yasuda Asako , Nakamura Kiminari , Sunada Katsuhisa 
Published: March 2020

Abstract
Background: Local anesthetic containing adrenaline is commonly used in many operations to maintain hemostasis and prolong the duration of anesthesia. Antipsychotic drugs have an α-adrenergic receptor blocking action, and it is thought that β-adrenergic receptors predominate when adrenaline is administered in combination, thus inducing hypotension. Many general anesthetics have vasodilatory effects, and it is possible that blood pressure may decrease further if adrenaline-containing lidocaine is administered to antipsychotic users during anesthesia. This study aimed to assess the circulation dynamics in regular antipsychotics users administered adrenaline-containing lidocaine under general anesthesia in a dental procedure. Methods: Participants included 30 patients regularly using antipsychotics (butyrophenone, phenothiazine, and/or atypical antipsychotics) who were scheduled for dental treatment to be performed under general anesthesia. At five minutes after tracheal intubation, the systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), and percutaneous oxygen saturation (SpO2) were measured as a baseline. SBP, DBP, HR, and SpO2 were measured 2, 4, 6, 8, and 10 minutes after the injection of 1.8 mL of 2% lidocaine (32 mg) with 1:80,000 adrenaline (22.5 μg) into the oral cavity. Differences in the SBP, DBP, HR, and SpO2 between baseline and at each time point were analyzed using Dunnett’s test.Results: No significant changes were observed in the SBP, DBP, HR, and SpO2 compared to baseline until ten minutes after the administration of adrenaline-containing lidocaine. No adverse events were observed up to one hour after the administration of adrenaline-containing lidocaine.Conclusions: This study demonstrated that adrenaline used at the usual dose for dental treatment under general anesthesia is unlikely to affect the circulation dynamics of regular antipsychotic users.
Assessing the impacts of the Agenda Gap intervention for youth mental health promotion through policy engagement: A study protocol
Authors: Jenkins Emily , Haines-Saah Rebecca , McGuinness Liza , Hirani Saima , Boakye-Yiadom Noah , Halsall Tanya , Rivers Robert , Morris Jonathan 
Published: March 2020

Abstract
Background Mental health challenges are a leading health concern for youth globally, requiring a comprehensive approach incorporating promotion, prevention and treatment within a healthy public policy framework. However, the broad enactment of this vision has yet to be realized. Further, mental health promotion evidence specific to youth is still emerging and has not yet focused at a policy level. This is a critical gap, as policy is a key mental health promotion lever that can alter the social and structural conditions that contribute to shaping youth mental health outcomes for all youth, across the full spectrum of need. Responsive to this research and intervention priority, our prototype study intervention – the Agenda Gap – is comprised of an innovative, multi-media engagement intervention, developed in collaboration with youth. This intervention aims to equip youth and build capacity for them to lead meaningful policy change reflective of the mental health needs of diverse communities of youth, including those who experience structural vulnerability and who would not typically have had their voice represented in policymaking processes. Methods A mixed methods study grounded in a realist approach will be conducted in three sites across two Canadian provinces (British Columbia and Alberta). In an earlier phase of this research, we collaboratively designed the prototype intervention with youth, community and policy partners. In this phase of the study, the intervention will be implemented and further tested with new groups of youth collaborators (n=10-15/site). Outcome data will be collected through realist qualitative interviews and validated questionnaires (i.e., Child and Youth Resilience Measure (CYRM-12), General Self-Efficacy (GSE) Scale, and the Critical Consiousness Scale (CCS)). Analysis will focus on identification of key context-mechanism-outcome configurations to provide comprehensive insights into how this intervention works, for whom, and in what context. Discussion This study is unique in its “upstream” focus on youth-engaged policymaking as a tool for improving the social and structural conditions that influence youth mental health across socioecological levels. Through the implementation and testing of the Agenda Gap intervention with diverse youth, this study will contribute to the evidence base on youth-engaged policymaking as a novel and innovative, mental health promotion strategy.
Factors associated with inappropriate attitude towards antibiotic usage among outpatients of a large public primary care center in Malaysia: A cross sectional study.
Authors: Zubaidi Zati Sabrina Ahmad , Malek Khasnur Abdul , Ismail Zaliha 
Published: March 2020

Abstract
Background: Antibiotic resistance is one of the biggest global threat in modern medicine. The situation is even more consequential in lower-and middle-income countries (LMIC) as financial limitations and political instability may become barriers to an impactful health policy. Therefore, The World Health organization has urge all nations to increase public awareness on antibiotic through effective educational interventions. In order for interventions to be successful, it is important to acknowledge that each country is unique in its problems as the demographic in LMIC are diverse. This study aims to identify the community’s attitude appropriateness towards antibiotic usage and their knowledge gaps, the relationship between antibiotic knowledge and their attitude as well as factors associated with inappropriate attitude towards antibiotic among outpatients attending an established primary care specialist center in Malaysia.Methodology : This cross-sectional study was conducted among 256 respondents attending a public primary care specialist clinic center. Those who fulfilled the inclusion and exclusion criteria from June to August 2017 were given a self-administered, validated and translated questionnaire. Pearson correlation was used to deduce the relationship between knowledge and attitude. Chi square test, independent t-test and multiple logistic regression were used to determine factors associated with inappropriate attitude towards antibiotic.Results: It was found that the respondents’ mean attitude and knowledge score were 29.5 ± 4.19 and 5.94 ± 2.4 respectively. The highest inappropriate attitude response was expecting antibiotic from the doctor for common colds and the most frequent incorrect knowledge response was on the domain of role of antibiotics. There is a weak positive relationship between antibiotic knowledge and attitude (r=0.315, n=256, p=0.0001). Respondents with low education level and poor antibiotic knowledge were at least 2.5 times more likely to have inappropriate attitude when handling antibiotics.Conclusion: Our study demonstrated the common inappropriate attitude towards antibiotic and areas of knowledge gap among the population. Antibiotic education should be incorporated in non-pharmacological measures of viral illnesses that is targeted among those with low education and poor antibiotic knowledge. The weak relationship between antibiotic knowledge and attitude suggest that health campaign should focus on behavioral change rather than a theoretical approach.
WITHDRAWN: Method and device for treating sinus pain at acupressure points
Authors: L Priya , S Ramesh , Aarthi S. , Preethi S. M. , Monisha T. 
Published: March 2020

Abstract
Sinusitis is an inflammation caused in the nasal passage due to cold, allergies or by nasal polyps. Sinusitis is a major problem among one in eight people around the world. People are interested more in ailment of natural treatments than antibiotic and other medicines. The objective of this work is to provide relief from sinusitis pain through acupressure points. The proposed methodology of sinusitis treatment can be done by applying heating cooling effect and massaging action simultaneously. This paper deals with both the methods of treating the sinusitis. This system integrates an android mobile application with a hardware device that serves this purpose. A face mask which consists of motors and heating and cooling system embedded which can be controlled using user friendly mobile application. The similar methodology can be used for other applications like back pain, muscular pain and knee pain relief.
Psychiatric inpatient beds for youths in China: data from a nation-wide survey
Authors: Geng Feng , Jiang Feng , Rakofsky Jeffrey , Liu Tingfang , Liu Yuanli , Liu Huanzhong , Tang Yi-lang 
Published: March 2020

Abstract
Background The development of child psychiatry in China is slow and very limited resources have been allocated to it. To investigate the current resources of inpatient psychiatric facilities for youth in top-tier psychiatric hospitals in China and the characteristics of youth patients hospitalized in an adult unit. Method As part of a official national survey, 29 most representative provincial tertiary psychiatric hospitals in China were selected. Data of 1975 inpatients discharged from these hospitals from March 19 to 31, 2019 were retrieved and analyzed. Results The mean number of youth psychiatric bed was 27.7±22.9 in these hospitals and 6/29 hospitals had zero youth beds. There were significantly more youth beds in developed regions than in less developed regions (P<0.05). Most of discharged youth patients were teenagers with severe mental illnesses, including schizophrenia, depressive disorder and bipolar disorder. 7.5% (149) of 1975 discharged patients were youth while youth beds only accounted for 3.2% (804/25136) of all psychiatric beds. 45.6% (68) of youth patients hospitalized in adult units. Conclusion Our findings highlight a dire situation of youth inpatient service in China, especially in less developed regions. There is an urgent need to change the policy and develop mental health services, including inpatient services for child and adolescent patients.
Mercury in the terrestrial environment: a review
Authors: Gworek Barbara , Dmuchowski Wojciech , Baczewska-Dąbrowska Aneta 
Published: March 2020

Abstract
Environmental contamination by mercury is and will continue to be a serious risk for human health. Pollution of the terrestrial environment is particularly important as it is a place of human life and food production. Publication presents a review of the literature on issues related to Hg pollution of the terrestrial environment: soil and plants and their transformations. Different forms of atmospheric Hg may be deposited on surfaces by way of wet and dry processes. These forms may be sequestered within terrestrial compartments or emitted back into the atmosphere, and the relative importance of these processes is dependent on the form of Hg, the surface chemistry, and the environmental conditions. On the land surface, Hg deposition mainly occurs in the oxidized form (Hg 2+ ), and its transformations are associated primarily with the oxidation-reduction potential of the environment and the biological and chemical processes of methylation. The deposition of Hg on ground with low vegetation as 3–5 times lower than that in forests. The estimation of Hg emissions from soil and plants, which occur mainly in the Hg 0 form, is very difficult. Generally, the largest amounts of Hg are emitted from tropical regions, and the lowest levels are from the polar regions.