bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex‐based differences in biomarkers, health status, and reverse cardiac remodelling in patients with heart failure with reduced ejection fraction treated with sacubitril/valsartan
Authors: Ibrahim Nasrien E. , Piña Ileana L. , Camacho Alexander , Bapat Devavrat , Felker G. Michael , Maisel Alan S. , Butler Javed , Prescott Margaret F. , Abbas Cheryl A. , Solomon Scott D. , Januzzi James L. ,   Prospective Study of Biomarkers, Symptom Improvement and Ventricular Remodeling During Entresto Therapy for Heart Failure (PROVE‐HF) Study Investigators
Published: September 2020

AimsWe sought to determine sex‐based differences in biomarkers, self‐reported health status, and magnitude of longitudinal changes in measures of reverse cardiac remodelling among patients with heart failure with reduced ejection fraction (HFrEF, left ventricular ejection fraction ≤40%) treated with sacubitril/valsartan (S/V).Methods and resultsThis was a subgroup analysis of patients initiated on S/V in the Prospective Study of Biomarkers, Symptom Improvement and Ventricular Remodeling During Entresto Therapy for Heart Failure (PROVE‐HF) study. There were 226 (28.5%) women in the study. Though women had lower baseline N‐terminal pro B‐type natriuretic peptide (NT‐proBNP), they had more rapid early reduction in the biomarker after initiation of S/V. Compared to men, women had lower average baseline Kansas City Cardiomyopathy Questionnaire (KCCQ)‐23 Total Symptom score (67.6 vs. 71.9; P = 0.003) but showed greater linear improvement (7.4 vs. 5.5 points; P < 0.001) and faster pace of KCCQ change (P < 0.001) over the course of the trial. Women and men demonstrated similar degrees of reverse left ventricular remodelling following S/V initiation; however, women did so earlier than men with more consistent changes. These results remained unchanged with adjustment for relevant covariates. Reduction in NT‐proBNP was associated with reverse cardiac remodelling in both women and men. Treatment with S/V was well tolerated in all.ConclusionsIn women with HFrEF, treatment with S/V was associated with significant NT‐proBNP reduction, health status improvement and reverse cardiac remodelling.
Effect of multi‐axial hot forging process on mechanical, and corrosion resistance behavior of Mg‐3Zn alloy for temporary orthopedic implants
Authors: Jaiswal Satish , Agrawal Shubham , Dubey Anshu , Lahiri Debrupa 
Published: September 2020

AbstractMagnesium (Mg) is a load‐bearing biocompatible material which has the ability to reduce stress shielding effect and facilitate osteocompatibility and biodegradation in the presence of body fluids. However, Mg is highly susceptible to corrosion in the physiological environment, hence leading to poor mechanical integrity. In this study, the multi‐axial hot forging (MAHF) process is performed on Mg‐3Zn alloys to study its grain refinement and possible improvement in mechanical, corrosion, and bioactivity behavior. The average grain size of the sample becomes significantly refined after the third cycle of MAHF. The yield and ultimate compressive strength of Mg‐3Zn alloys are found to increase by 70% and 41%, respectively, after the third cycle of MAHF, which is potentially due to the grain size refinement. Accelerated corrosion studies show improvements in the corrosion resistance with the refined grain structure. Additionally, in‐vitro immersion studies in the simulated body fluid for 14 days showed a reduction in the degradation rate after third cycle of MAHF, due to the increased grain boundary area, which offered more nucleation sites for apatite precipitation. This study underscores and lays the foundation for a new branch of severely deformed fine‐grained Mg‐3Zn alloy for temporary orthopedic implants.
Felt power: Can Mexican Indigenous women finally be powerful?
Authors: Whittaker Catherine 
Published: September 2020

AbstractToo often, women from the Global South have been portrayed as victims of gender violence in need of empowerment. Yet in the rural south of Mexico City, many Indigenous women expressed feeling strong, even powerful, despite their varied experiences of violence. Based on fifteen months of ethnographic fieldwork, I argue that their felt power was often rooted in their realities. Nahuatl‐speaking communities have historically recognized certain kinds of power in women (including healing magic) and others in men (including political power). Women's narratives revealed complex interactions between women's and men's power. Women often represented themselves not as helpless victims but as having the power to change their circumstances, for better or worse. I introduce felt power as a conceptual tool for centering Indigenous women's experiential, embodied, and spiritual knowledge in addressing the gender‐based violence they often experienced. Felt power is derived from Dian Million's framework of felt theory, which represents Indigenous people's narratives as feeling‐based theory‐making, rather than raw data to be theorized into abstraction by non‐Indigenous thinkers. I suggest that considering and respecting Indigenous women's felt power in the face of violence will contribute to decolonizing the study of gender violence and development agencies’ responses to it.
A meta‐analysis: Does vitamin D play a promising role in sleep disorders?
Authors: Yan Shoumeng , Tian Zhenwei , Zhao Hantong , Wang Changcong , Pan Yingan , Yao Nan , Guo Yinpei , Wang Han , Li Bo , Cui Weiwei 
Published: September 2020

AbstractBackgroundSleep disorders, one of the most common problems in the general population, have been related to a series of harmful health consequences. Vitamin D appears to be associated with sleep disorders. However, the difference in vitamin D levels between sleep disorder subjects and people without a sleep disorder is unclear. Simultaneously, the influence of vitamin D replenishment on sleep disorders remains controversial.MethodsPubMed, MEDLINE, Web of Science, and Cochrane Library were searched for literatures published until October 2019. Using a random effects model, a meta‐analysis was conducted to calculate the standard mean difference to evaluate the difference in vitamin D concentrations between sleep disorder subjects and normal people and the efficacy of vitamin D supplementation on sleep disorders.ResultsOur study found that the serum vitamin D levels in the sleep disorder subjects were lower than that in the normal people (SMD = −0.75 ng/ml, 95% CI = −0.93, −0.57 ng/ml). Moreover, the Pittsburgh Sleep Quality Index (PSQI)in the subjects with vitamin D supplementation was lower than that in the controls (SMD = −0.45, 95% CI = −0.76, −0.13).ConclusionsVitamin D could play a promising role in sleep disorders. More data are required to confirm the efficacy of vitamin D supplementation for improving sleep disorders.
Physicochemical composition, lipid oxidation, and microbiological quality of ram mortadella supplemented with Smallanthus sonchifolius meal
Authors: do Santos Junior Alexandre Cristiano , Oliveira Rodrigo Fortunato de , Henry Fabio da Costa , Maia Junior Jonhny de Azevedo , Moulin Monique Moreira , Della Lucia Suzana Maria , Quirino Célia Raquel , Martins Meire Lelis Leal , Rampe Maria Cecília Cabral 
Published: September 2020

AbstractThis study evaluated the physicochemical, lipid peroxidation, and microbiological quality of mortadellas prepared with ram and supplemented with different amounts of Smallanthus sonchifolius meal. Three mutton mortadella formulations supplemented with 1.25%, 2.50%, and 5% yacón meal and control formulation without yacón meal was included. The physicochemical, lipid peroxidation, and microbiological analyses were carried out in the time periods 10, 45 days, three, and six month after the preparation of mortadella. The control formulation presenting lighter and more intense red tone compared with the other formulations. All formulations presenting lipid peroxidation increased 90 days after processing; already the pH and Aw values were constant for all formulations at the experimental times stipulated. All formulations had the physicochemical characterization and microbiological quality standards, according to defined in regulations for mortadella production in Brazil. The results show that mutton mortadella supplemented with yacón meal is a promising alternative in the manufacture of healthy meat products.
Orthogonal arrays of particle assembly are essential for normal aquaporin‐4 expression level in the brain
Authors: de Bellis Manuela , Cibelli Antonio , Mola Maria Grazia , Pisani Francesco , Barile Barbara , Mastrodonato Maria , Banitalebi Shervin , Amiry‐Moghaddam Mahmood , Abbrescia Pasqua , Frigeri Antonio , Svelto Maria , Nicchia Grazia Paola 
Published: September 2020

AbstractAstrocyte endfeet are endowed with aquaporin‐4 (AQP4)‐based assemblies called orthogonal arrays of particles (OAPs) whose function is still unclear. To investigate the function of OAPs and of AQP4 tetramers, we have generated a novel “OAP‐null” mouse model selectively lacking the OAP forming M23‐AQP4 isoform. We demonstrated that AQP4 transcript levels were not reduced by using qPCR. Blue native (BN)/SDS‐PAGE and Western blot performed on OAP‐null brain and primary astrocyte cultures showed the complete depletion of AQP4 assemblies, the selective expression of M1‐AQP4‐based tetramers, and a substantial reduction in AQP4 total expression level. Fluorescence quenching and super‐resolution microscopy experiments showed that AQP4 tetramers were functionally expressed in astrocyte plasma membrane and their dimensions were reduced compared to wild‐type assemblies. Finally, as shown by light and electron microscopy, OAP depletion resulted in a massive reduction in AQP4 expression and a loss of perivascular AQP4 staining at astrocyte endfeet, with only sparse labeling throughout the brain areas analyzed. Our study relies on the unique property of AQP4 to form OAPs, using a novel OAP‐null mouse model for the first time, to show that (a) AQP4 assembly is essential for normal AQP4 expression level in the brain and (b) most of AQP4 is organized into OAPs under physiological conditions. Therefore, AQP4 tetramers cannot be used by astrocytes as an alternative to OAPs without affecting AQP4 expression levels, which is important in the physiological and pathological conditions in which OAP aggregation/disaggregation dynamics have been implicated.
Neuroinflammatory inhibition of synaptic long‐term potentiation requires immunometabolic reprogramming of microglia
Authors: York Elisa M. , Zhang Jingfei , Choi Hyun B. , MacVicar Brian A. 
Published: September 2020

AbstractImmunometabolism refers to the rearrangement of metabolic pathways in response to immune stimulation, and the ability of these metabolic pathways themselves to control immune functions. Many aspects of immunometabolism have been revealed through studies of peripheral immune cells. However, immunometabolic reprogramming of microglia, the resident immune cell of the central nervous system, and the consequential outcome on neuronal activity have remained difficult to unravel. Microglia are highly sensitive to subtle changes in their environment, limiting the techniques available to study their metabolic and inflammatory profiles. Here, using fluorescence lifetime imaging of endogenous NAD(P)H, we measure the metabolic activity of individual microglia within acute hippocampal slices. We observed an LPS‐induced increase in aerobic glycolysis, which was blocked by the addition of 5 mM 2‐deoxyglucose (2DG). This LPS‐induced glycolysis in microglia was necessary for the stabilization of hypoxia inducible factor‐1α (HIF‐1α) and production of the proinflammatory cytokine, interleukin‐1β (IL‐1β). Upon release, IL‐1β acted via the neuronal interleukin‐1 receptor to inhibit the formation of synaptic long‐term potentiation (LTP) following high frequency stimulation. Remarkably, the addition of 2DG to blunt the microglial glycolytic increase also inhibited HIF‐1α accumulation and IL‐1β production, and therefore rescued LTP in LPS‐stimulated slices. Overall, these data reveal the importance of metabolic reprogramming in regulating microglial immune functions, with appreciable outcomes on cytokine release and neuronal activity.
Prognostic models integrating quantitative parameters from baseline and interim positron emission computed tomography in patients with diffuse large B‐cell lymphoma: post‐hoc analysis from the SAKK38/07 clinical trial
Authors: Zucca Emanuele , Cascione Luciano , Ruberto Teresa , Facchinelli Davide , Schär Sämi , Hayoz Stefanie , Dirnhofer Stefan , Giovanella Luca , Bargetzi Mario , Mamot Christoph , Ceriani Luca 
Published: September 2020

AbstractPositron emission computed tomography (PET/CT) in patients with diffuse large B‐cell lymphoma (DLBCL) enrolled in a prospective clinical trial were reviewed to test the impact of quantitative parameters from interim PET/CT scans on overall (OS) and progression‐free (PFS) survival. We centrally reviewed baseline and interim PET/CT scans of 138 patients treated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone given every 14 days (R‐CHOP14) in the SAKK38/07 trial (ClinicalTrial.gov identifier: NCT00544219). Cutoff values for maximum standardized uptake value (SUVmax), metabolic tumor volume (MTV), total lesion glycolysis (TLG) and metabolic heterogeneity (MH) were defined by receiver operating characteristic analysis. Responses were scored using the Deauville scale (DS). Patients with DS 5 at interim PET/CT (defined by uptake >2 times higher than in normal liver) had worse PFS (P = 0.014) and OS (P < 0.0001). A SUVmax reduction (Δ) greater than 66% was associated with longer PFS (P = 0.0027) and OS (P < 0.0001). Elevated SUVmax, MTV, TLG, and MH at interim PET/CT also identified patients with poorer outcome. At multivariable analysis, ΔSUVmax and baseline MTV appeared independent outcome predictors. A prognostic model integrating ΔSUVmax and baseline MTV discriminated three risk groups with significantly (log‐rank test for trend, P < 0.0001) different PFS and OS. Moreover, the integration of MH and clinical prognostic indices could further refine the prediction of OS. PET metrics‐derived prognostic models perform better than the international indices alone. Integration of baseline and interim PET metrics identified poor‐risk DLBCL patients who might benefit from alternative treatments.
DNMT1 maintains the methylation of miR‐152‐3p to regulate TMSB10 expression, thereby affecting the biological characteristics of colorectal cancer cells
Authors: Wang Chenchen , Ma Xiaoji , Zhang Jieyun , Jia Xiaobin , Huang Mingzhu 
Published: September 2020

AbstractObjectiveDNA methyltransferases (DNMTs) take on a relevant role in epigenetic control of cancer proliferation and cell survival. However, the molecular mechanisms underlying the establishment and maintenance of DNA methylation in human cancer remain to be fully elucidated. This study was to investigate that how DNMT1 affected the biological characteristics of colorectal cancer (CRC) cells via modulating methylation of microRNA (miR)‐152‐3p and thymosin β 10 (TMSB10) expression.MethodsDNMT1, miR‐152‐3p, and TMSB10 expression, and the methylation of miR‐152‐3p in CRC tissues and cells were detected. SW‐480 and HCT‐116 CRC cells were transfected with DNMT1 or miR‐152‐3p‐related sequences or plasmids to explore their characters in biological functions of CRC cells. The binding relationship between DNMT1 and miR‐152‐3p and the targeting relationship between miR‐152‐3p and TMSB10 were analyzed. The tumor growth was also detected in vivo.ResultsUpregulated DNMT1, TMSB10, reduced miR‐152‐3p, and methylated miR‐152‐3p were detected in CRC tissues and cells. Silenced DNMT1 or upregulated miR‐152‐3p reduced TMSB10 expression and suppressed CRC progression and tumor growth. Moreover, elevated DNMT1 could reverse the effect of miR‐152‐3p upregulation on CRC development and tumor growth. DNMT1 maintained methylation of miR‐152‐3p. TMSB10 was the direct target gene of miR‐152‐3p.ConclusionThe study highlights that silenced DNMT1 results in non‐methylated miR‐152‐3p to depress TMSB10 expression, thereby inhibiting CRC development, which provides a new approach for CRC therapy.
Synthesis, in vitro cytotoxicity, ADME, and molecular docking studies of benzimidazole‐bearing furanone derivatives
Authors: Husain Asif , Bhutani Medha , Parveen Shazia , Khan Shah Alam , Ahmad Aftab , Iqbal Md Azhar 
Published: September 2020

AbstractA series of benzimidazole‐derived–furanones (4a–l) were synthesized, characterized, and explored for their in vitro anticancer activities. The pharmacokinetic parameters assessed revealed that all the compounds followed the Lipinski's rule of five, making them potential drug candidates. Further, the results of anticancer activity revealed that (E)‐5‐(1H‐benzo[d]imidazol‐2‐yl)‐3‐(3,4,5‐trimethoxybenzylidene)furan‐2(3H)‐one (4a), was active against A549, MCF7, and DU145 with an IC50 values of 10.4 ± 0.39, 11.1 ± 0.43, and 10.7 ± 0.19 μM, respectively. While another compound (E)‐5‐(1H‐benzo[d]imidazol‐2‐yl)‐3‐([5‐phenyl]furan‐2‐yl)furan‐2(3H)‐one (4k) also exhibited good activity against A549, MCF7, and DU145 with IC50 values of 11.4 ± 0.39, 9.1 ± 0.43, and 12.7 ± 0.19 μM, respectively. Doxorubicin was used as the standard drug. Further, molecular docking studies were carried out to provide binding mode into the binding sites of vascular endothelial growth factor receptor (VEGFR). Docking scores and binding energies corroborated well with the results of experimental anticancer activity. Pharmacokinetic (ADME) parameters of the potent derivatives were also found to be in an acceptable range. The benzimidazole‐furanonone conjugates seem to be a potential source for the further development of potent cytotoxic agents.
Meaning profiles and the perception of the working alliance at the start of outpatient person‐centered, experiential, and existential psychotherapies
Authors: Golovchanova Nadezhda , Dezutter Jessie , Vanhooren Siebrecht 
Published: September 2020

AbstractObjective(s)Quantitative research on meaning in life in the context of psychotherapy is relatively limited. The current study aims to investigate the profiles of the meaning of clients and their perception of the working alliance and initial symptomatology at the start of therapy.DesignIn a sample of 145 clients (62.1% female; mean age, 34.77) who started person‐centered psychotherapy, the relationship between meaning, search for meaning, symptomatology, and the working alliance was analyzed. The assessment took place after the second session.ResultsCluster analysis revealed four profiles: Low Presence High Search, High Presence Low Search, High Presence High Search, and Low Presence Low Search. These meaning profiles are distinguished in terms of symptomatology but not in terms of the working alliance perception. However, the experience of meaning is significantly associated with the working alliance.ConclusionsResults show the relevance of meaning‐related questions for certain groups of clients at the start of therapy.
Validation of a description of sarcopenic obesity defined as excess adiposity and low lean mass relative to adiposity
Authors: Baker Joshua F. , Harris Tamara , Rapoport Allegra , Ziolkowski Susan L. , Leonard Mary B. , Long Jin , Zemel Babette , Weber David R. 
Published: September 2020

AbstractBackgroundThis study aims to assess the construct validity of a body composition‐defined definition of sarcopenic obesity based on low appendicular lean mass relative to fat mass (ALMIFMI) and high fat mass index (FMI) and to compare with an alternative definition using appendicular lean mass index (ALMI) and percent body fat (%BF).MethodsThis is a secondary analysis of two cohort studies: the National Health and Examination Survey (NHANES) and the Health, Aging, and Body Composition study (Health ABC). Sarcopenic obesity was defined as low ALMIFMI combined with high FMI and was compared with a widely used definition based on ALMI and %BF cut‐points. Body composition Z‐scores, self‐reported disability, physical functioning, and incident disability were compared across body composition categories using linear and logistic regression and Cox proportional hazards models.ResultsAmong 14, 850 participants from NHANES, patients with sarcopenic obesity defined by low ALMIFMI and high FMI (ALMIFMI‐FMI) had above‐average FMI Z‐scores [mean (standard deviation): 1.00 (0.72)]. In contrast, those with sarcopenic obesity based on low ALMI and high %BF (ALMI‐%BF) had below‐average FMI Z‐scores. A similar pattern was observed for 2846 participants from Health ABC. Participants with sarcopenic obesity based on ALMIFMI‐FMI had a greater number of disabilities, worse physical function, and a greater risk of incident disability compared with those defined based on ALMI‐%BF.ConclusionsBody composition‐defined measures of sarcopenic obesity defined as excess adiposity and lower‐than‐expected ALMI relative to FMI are associated with functional deficits and incident disability and overcome the limitations of using %BF in estimating obesity in this context.
Cancer cachexia in a mouse model of oxidative stress
Authors: Brown Jacob L. , Lawrence Marcus M. , Ahn Bumsoo , Kneis Parker , Piekarz Katarzyna M. , Qaisar Rizwan , Ranjit Rojina , Bian Jan , Pharaoh Gavin , Brown Chase , Peelor Fredrick F. , Kinter Michael T. , Miller Benjamin F. , Richardson Arlan , Van Remmen Holly 
Published: September 2020

AbstractBackgroundCancer is associated with muscle atrophy (cancer cachexia) that is linked to up to 40% of cancer‐related deaths. Oxidative stress is a critical player in the induction and progression of age‐related loss of muscle mass and weakness (sarcopenia); however, the role of oxidative stress in cancer cachexia has not been defined. The purpose of this study was to examine if elevated oxidative stress exacerbates cancer cachexia.MethodsCu/Zn superoxide dismutase knockout (Sod1KO) mice were used as an established mouse model of elevated oxidative stress. Cancer cachexia was induced by injection of one million Lewis lung carcinoma (LLC) cells or phosphate‐buffered saline (saline) into the hind flank of female wild‐type mice or Sod1KO mice at approximately 4 months of age. The tumour developed for 3 weeks. Muscle mass, contractile function, neuromuscular junction (NMJ) fragmentation, metabolic proteins, mitochondrial function, and motor neuron function were measured in wild‐type and Sod1KO saline and tumour‐bearing mice. Data were analysed by two‐way ANOVA with Tukey–Kramer post hoc test when significantFratios were determined andαwas set at 0.05. Unless otherwise noted, results in abstract are mean ±SEM.ResultsMuscle mass and cross‐sectional area were significantly reduced, in tumour‐bearing mice. Metabolic enzymes were dysregulated in Sod1KO mice and cancer exacerbated this phenotype. NMJ fragmentation was exacerbated in tumour‐bearing Sod1KO mice. Myofibrillar protein degradation increased in tumour‐bearing wild‐type mice (wild‐type saline, 0.00847 ± 0.00205; wildtype LLC, 0.0211 ± 0.00184) and tumour‐bearing Sod1KO mice (Sod1KO saline, 0.0180 ± 0.00118; Sod1KO LLC, 0.0490 ± 0.00132). Muscle mitochondrial oxygen consumption was reduced in tumour‐bearing mice compared with saline‐injected wild‐type mice. Mitochondrial protein degradation increased in tumour‐bearing wild‐type mice (wild‐type saline, 0.0204 ± 0.00159; wild‐type LLC, 0.167 ± 0.00157) and tumour‐bearing Sod1KO mice (Sod1KO saline, 0.0231 ± 0.00108; Sod1 KO LLC, 0.0645 ± 0.000631). Sciatic nerve conduction velocity was decreased in tumour‐bearing wild‐type mice (wild‐type saline, 38.2 ± 0.861; wild‐type LLC, 28.8 ± 0.772). Three out of eleven of the tumour‐bearing Sod1KO mice did not survive the 3‐week period following tumour implantation.ConclusionsOxidative stress does not exacerbate cancer‐induced muscle loss; however, cancer cachexia may accelerate NMJ disruption.