bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ_LDLR promoted the development of papillary thyroid carcinoma via regulating miR-195-5p/LIPH axis
Authors: Gui Xiaolong , Li Yan , Zhang Xiaobin , Su Ka , Cao Wenlong 
Published: June 2020

Abstract
Background: Emerging studies have demonstrated that circular RNAs (circRNAs) are key regulators for tumorigenesis in cancers, including papillary thyroid carcinoma (PTC). In this study, we aimed to explore the effects of circ_LDLR on PTC. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the levels of circ_LDLR, miR-195-5p and lipase H (LIPH). RNase R digestion assay and Actinomycin D assay were utilized to analyze the characteristics of circ_LDLR. Colony formation assay and 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay were conducted to evaluate cell proliferation. Western blot assay was used for the determination of protein levels. Flow cytometry analysis was applied to determine cell apoptosis. Transwell assay was performed to determine cell migration and invasion. Dual-luciferase reporter assay was used to verify the associations among circ_LDLR, miR-195-5p and LIPH. The murine xenograft model was constructed to explore the roles of circ_LDLR in vivo. Results: Compared to normal tissues and cells, circ_LDLR was upregulated in PTC tissues and cells. Silencing of circ_LDLR suppressed PTC cell colony formation, proliferation, migration and invasion and promoted apoptosis in vitro and hampered tumor growth in vivo. For mechanism investigation, circ_LDLR could regulate LIPH expression via sponging miR-195-5p. Moreover, miR-195-5p inhibition restored the effects of circ_LDLR knockdown on the malignant behaviors of PTC cells. MiR-195-5p overexpression inhibited PTC cell colony formation, proliferation, migration and invasion and facilitated apoptosis by targeting LIPH. Conclusion: Circ_LDLR knockdown decelerated PTC progression by regulating miR-195-5p/LIPH axis, which might provide a novel therapeutic target for PTC.
Infrequent tooth brushing and teeth lost may increase the risk of oral and pharyngeal cancers: A nationwide population-based cohort study in Korea.
Authors: Woo Ho Geol , Lee Kijeong , Lee Ji Sung , Kim Jinkwon , Chang Younkyung , Kim Jin-Woo , Song Tae Jin 
Published: June 2020

Abstract
Background: Oral and pharyngeal cancers are emerging and troublesome diseases worldwide. Although poor oral hygiene behaviors and parameters affecting oral health have been considered as potential risk factors of oral and pharyngeal cancers including smoking and alcohol consumption for decades, study results have been inconsistent. Longitudinal studies regarding the association between poor oral hygiene behaviors and parameters of oral health and the occurrence of oral and pharyngeal cancers have been lacking. We aimed to investigate this relationship in a nationwide general population-based cohort.Methods: We included data on demographics, past history, and laboratory findings for 150,774 subjects without teeth lost from the Korean National Health Insurance System-Health Screening Cohort. The presence of periodontal disease and parameters of oral health, including frequency of tooth brushings, dental visits for any reason, professional teeth scaling, and number of teeth lost, were investigated. Oral and pharyngeal cancers were defined by the International Statistical Classification of Diseases, 10th Revision, codes of C00-C14.Results: During a median 11.1 years of follow-up, 1,155 (0.77%) oral and pharyngeal cancers occurred. Based on multivariable analysis, after adjusting for demographics, alcohol consumption, smoking status, regular exercise, body mass index, systolic blood pressure, blood and urinary laboratory findings, and parameters of oral health including frequent tooth brushing (three or more times per day) were related to a lower risk of oral and pharyngeal cancers (hazard ratio [HR]: 0.78, 95% confidence interval [CI]: 0.66–0.93, p=0.005). An increased number of teeth lost (≥15) was positively associated with occurrence of oral and pharyngeal cancers (HR: 1.71, 95% CI: 1.26–2.31, p=0.001 in model 1; HR: 1.68, 95% CI: 1.24–2.28, p<0.001 in model 2; HR: 1.66, 95% CI: 1.22–2.25, p=0.001 in model 3; p-value for trend test for HR <0.001).Conclusions: The occurrence of oral and pharyngeal cancers decreased with more frequent tooth brushing (three or more times per day) and increased with the number of teeth lost. With regards to the prevention of oral and pharyngeal cancers, improving oral hygiene may have a significant contribution.
Supporting Australian Clinical Learners in a Collaborative Clusters Education Model: A Mixed Methods Study
Authors: de Mortel Thea van , Armit Lyn , Shanahan Brenton , Needham Judith , Brown Candy , Grafton Eileen , Havell Michelle , Henderson Amanda , Grealish Laurie 
Published: June 2020

Abstract
Background: Nursing student numbers have risen in response to projected registered nurse shortfalls, increasing numbers of new graduates requiring transitional support and pressure on clinical placements. A Collaborative Clusters Education Model, in which Entry to Practice facilitators coach ward-based registered nurses to support students’ and new graduates’ learning, may address placement capacity. The research aim was to evaluate the acceptability of the Collaborative Clusters Education Model to stakeholders by examining their perceptions of the facilitators and barriers to the model in its implementation. Methods: A convergent mixed methods evaluation approach was adopted. The study took place in a large Australian health service in south-east Queensland. Participants included Bachelor of Nursing students, Entry to Practice facilitators, ward-based registered nurses, academics and new graduates. A mixed methods design was used. Elements included an online survey of nursing students, and interviews with new graduates, Entry to Practice facilitators, ward-based registered nurses, and academics. Descriptive statistics were calculated on quantitative data. Thematic analysis was conducted on qualitative data. Results: Participants included 134 (of 990) nursing students (response rate 13.5%), five new graduates, seven Entry to Practice facilitators, four registered nurses, and three nurse academics. Students rated facilitators’ effectiveness highly (4.43/5±0.75), although this finding is tempered by a low response rate (13.5%). For learners, the model provided access to learning experiences, although preferences for sources of support differed between students and new graduates, and further clarification of responsibilities was required. For other stakeholders, three themes emerged: students’ and new graduates’ integration into the workplace can promote learning; tensions arise in new ways to approach performance assessment; and aligning expectations requires high levels of communication. Conclusions: This evaluation found that acceptability was good but at risk from limited clarity around roles and responsibilities. Further research into this model is recommended.
Motivation factors affecting the job attitude of medical doctors and the organizational performance of public hospitals in Warsaw, Poland
Authors: Chmielewska Malgorzata , Stokwiszewski Jakub , Filip Justyna , Hermanowski Tomasz 
Published: June 2020

Abstract
Background This paper explores the relationship between selected motivation factors that affect the attitude to work among medical doctors at public hospitals and the organizational performance of hospitals and selected motivation factors that affect the attitude to work among medical doctors at public hospitals. Methods This study was based on World Health Organization questionnaires designed to estimate motivation factors according to Herzberg’s motivation theory and to measure the levels of organizational performance of hospitals using the McKinsey model in the social aspect. A survey was conducted among physicians (n=249) with either surgical or nonsurgical specialty in 22 departments/units of general public hospitals in Warsaw, Poland. The relationship between the examined factors of job motivation and organizational effectiveness was determined using Spearman’s rank correlation. Furthermore, 95% confidence intervals were computed. The independent samples t-test was used to confirm any statistically significant differences between the independent groups. Normality was tested by the Kolmogorov–Smirnov test. Results The survey revealed that motivation factors related to “quality and style of supervision” have the greatest effect on the hospital’s organizational performance (Spearman’s rank correlation coefficient = 0.490; p<0.001), whereas “performance feedback” was rated as the lowest to affect organizational performance among the surveyed healthcare professionals (54% of physicians). Conclusion The principles of Individual Performance Review should be incorporated into strategies designed to improve the organizational performance of hospitals (with NHS serving as a possible role model) in order to establish specific rules on how to share performance feedback with among individual physicians. The present study contributes to literature on human resource management in the healthcare sector, highlighting the importance of social nonfinancial aspects in improving organizational performance in a hospital setting.
The effects of seaward distance on above and below ground carbon stocks in estuarine mangrove ecosystems
Authors: Cleyndert Georgia de Jong , Cuni-Sanchez Aida , Seki Hamidu A , Shirima Deo D , Munishi Pantaleo K.T. , Burgess Neil , Marchant Robert 
Published: June 2020

Abstract
Background: Mangrove forests have gained recognition for their potential role in climate change mitigation due to the carbon sequestration of the productive ecosystems and storage in the sediments that are trapped by the mangrove tree roots and pneumatophores. Africa hosts about 19% of the world’s mangroves, yet there are relatively few studies that have examined the carbon stocks of African mangroves, and the studies available report great differences among sites and amongst the different pools of carbon stocks, particularly between the above ground carbon stored in trees (AGC) and organic carbon stored within the sediment - ‘soil organic carbon (SOC)’ and none considered the effects of seaward distance. We investigate if AGC and SOC carbon stocks differ with increasing seaward distance for estuarine mangroves in Tanzania and, how our results compare to those reported elsewhere across Africa.Results: AGC ranged between 10.9-54.9 Mg C ha-1 , but was not significantly affected by seaward distance. SOC for 0-1m depth ranged from 153.7-483.6, with a mean of 301.7 Mg C ha-1. SOC was significantly negatively correlated with seaward distance, which is different from studies in Micronesia and the Indo-Pacific. Mangrove type (estuarine/oceanic), soil erosion and soil depth may explain these differences We note important methodological differences in previous studies on carbon stocks in mangroves in Africa. Conclusion:This study indicates that seaward distance has an important effect on SOC stocks in the Lindi region of Tanzania. There is the need to standardise methodological approaches for mangroves in Africa, to report seaward distance and to describe the type of mangrove stand to make results easily comparable across sites and to assess the true value of Blue Carbon in Africa. We recommend focusing on trees >10cm diameter for AGC, and sampling soils to greater than 1m depth for SOC, which would provide a more complete assessment of the potentially considerable mangrove carbon store.
COVID-19 Pandemic- Knowledge, Perception, Anxiety and Depression Among Frontline Doctors of Pakistan
Authors: Amin Faridah , Sharif Salman , Saeed Rabeeya , Durrani Noureen , Jilani Daniyal 
Published: June 2020

Abstract
BackgroundCOVID-19 is a global pandemic and has become a major public health burden worldwide. With already fragile healthcare systems it can have long lasting effects in developing countries. Outbreaks especially a pandemic situation evokes fear related behaviors among healthcare professionals and there is always an increased risk of mental health disorders. Therefore, this study aims to determine knowledge and perception about this pandemic, prevalence and factors associated with depression/ anxiety among frontline physicians of Pakistan.MethodsData were collected through an online survey released in the last week of March-2020. 389 frontline physicians from all four provinces and 65 cities of Pakistan participated. Survey questionnaire consisted of 4 parts including informed consent section, demographic section, knowledge and perception about COVID-19 pandemic and assessment of depression through World Health Organization Self-reporting questionnaire (SRQ-20). A score of 8 or above on SRQ-20 was used as cut-off to label the participant as depressed. Data was analyzed using SPSS version22.ResultsA 43% prevalence of depression/anxiety among frontline physicians of Pakistan was reported. Almost all the doctors had moderate to high knowledge score. Majority of participants marked N-95 mask as essential while aerosol generating procedures, assessing patients with respiratory symptoms, in COVID patient care area, ER triage and direct care of COVID-19 patient. Only 12% of the doctors were fully satisfied with the provision of PPEs and almost 94% felt unprotected.In multivariable model, the odds of depression was high in physicians who were assessing more than five COVID suspects/patients per day (aOR=2.73, 95% CI: 1.65 – 4.52), who were working 20 hours/week or less (aOR=2.11, 1.27 – 3.49), had children among household members (aOR= 1.58, 95% CI: 1.00 – 2.50) and had moderate to low knowledge of the infection (aOR=2.69, 95% CI: 1.68 – 4.31).ConclusionDepression/anxiety among more than a third of frontline doctors of Pakistan warrants the need to address mental health of doctors caring for patients during this pandemic; control modifiable factors associated with it and explore the effectiveness of interventions to promote psychological well-being of physicians.
Antibacterial mechanism of rhodomyrtone involves the disruption of nucleoid segregation checkpoint in Streptococcus suis
Authors: Traithan Apichaya , Tongtawe Pongsri , Thanongsaksrikul Jeeraphong , Voravuthikunchai Supayang , Sriman Potjanee 
Published: June 2020

Abstract
Rhodomyrtone has been recently demonstrated to possess a novel antibiotic mechanism of action against Gram-positive bacteria which involved the multiple targets, resulting in the interference of several bacterial biological processes including the cell division. The present study aims to closely look at the downstream effect of rhodomyrtone treatment on nucleoid segregation in Streptococcus suis, an important zoonotic pathogen. The minimum inhibition concentration (MIC) and the minimum bactericidal concentration (MBC) values of rhodomyrtone against the recombinant S. suis ParB-GFP, a nucleoid segregation reporter strain, were 0.5 and 1 µg/ml, respectively, which were equivalent to the potency of vancomycin. Using the fluorescence live-cell imaging, we demonstrated that rhodomyrtone at 2 × MIC caused incomplete nucleoid segregation and septum misplacement, leading to the generation of anucleated cells. FtsZ immune-staining of rhodomyrtone-treated S. suis for 30 min revealed that the large amount of FtsZ was trapped in the region of high fluidity membrane and appeared to be able to polymerize to form a complete Z-ring. However, the Z-ring was shifted away from the midcell. Transmission electron microscopy further confirmed the disruption of nucleoid segregation and septum misplacement at 120 min following the rhodomyrtone treatment. Asymmetric septum formation resulted in either generation of minicells without nucleoid, septum formed over incomplete segregated nucleoid (guillotine effect), or formation of multi-constriction of Z-ring within a single cell. This finding spotlights on antibacterial mechanism of rhodomyrtone involves the early stage in bacterial cell division process.
Unhealthy lifestyle is an important risk factor for BPPV recurrence
Authors: Fu Chang-yong , Zhang Zhen-zhong , Chen Jin , Jaiswal Sandip Kumar , Yan Fu-ling 
Published: June 2020

Abstract
Background: Benign paroxysmal positional vertigo (BPPV) is self-limiting and recurrent but the cost is considerable. The recurrence of BPPV increased significantly under the quarantine policy in Hangzhou. The unhealthy lifestyle risk factors of the recurrence of BPPV have not yet been investigated. So, the objective is to analyze the unhealthy lifestyle risk factors of recurrent BPPV.Methods: A retrospective observational study was conducted in the Department of Neurology in Tongde Hospital of Zhejiang Province from December 2018 to March 2020. The study included 186 patients aged 23–86 years. All patients received the definitive diagnosis and canalith repositioning maneuvers (CRM) treatment and finally accomplished follow-up for one year. Demographic variables, potential recurrence risk factors, neurological examination, cranial computed tomography and lifestyle were assessed.Data Analyses: The t-test or chi-squared test was first performed for group comparison, then logistic regression analysis was performed to investigate the recurrence risk factors in all of the patients.Results: The 1-year recurrence rates of BPPV patients after reposition maneuvers were 36.02%, is significantly higher than other study especially in the first quarter of 2020 (38.8% of patients with BPPV recurrence). Logistic regression analysis shows that BPPV patients who was poor physical activities and prolonged recumbent position time have more recurrence risks. Poor physical activities (odds ratio =5.690, 95% confidence interval: 1.650-19.618, p <0.006), prolonged recumbent position time (odds ratio = 1.651, 95% confidence interval: 1.370-2.003, p = 0.000), were risk factors for the recurrence of BPPV in patients.Conclusion: We conclude that poor physical activities and prolonged recumbent position time may be independent risk factors for the recurrence of BPPV patients, but aging, Ménière’s disease, sudden deafness migraine, hypertension, hyperlipemia, diabetes, CHD, POCI, mental factors, and mental labor do not increase the recurrence risk.
Clinical Characteristics, Treatment and Outcome of Adnexal Torsion in Pregnant Women: A Retrospective Study
Authors: Deng Shan , Wang Yong-xue 
Published: June 2020

Abstract
Background Adnexal torsion during pregnancy is a gynecological emergency. Delayed diagnosis and treatment can cause ovarian necrosis and fetal loss. This study assessed the clinical characteristics, treatment and outcomes of adnexal torsion in pregnant women.Methods A retrospective study was conducted at a tertiary center between January 2008 and January 2018. Eighty-two pregnant women with surgically confirmed adnexal torsion were included. The clinical characteristics, ultrasound data, surgical interventions and pregnancy outcomes were analyzed.Results The median age of the patients was 28 (range, 18–38) years. The median gestational age was 11 (range, 6–31) weeks: 53 (64.6%) were in the first trimester, 21 (25.6%) were in the second trimester, and 8 (9.8%) were in the third trimester. The most common symptoms and signs were sudden pelvic pain (100%) and adnexal or pelvic masses (97.6%), followed by nausea and vomiting (61%). The Doppler blood flow signal disappeared in 62.5% of the patients. Sixty-three (76.8%) patients underwent laparoscopy, and 29 (24.2%) underwent laparotomy. The median gestational age in patients undergoing laparotomy was higher than that in those undergoing laparoscopy (26 weeks vs 10 weeks, p < 0.001). Fifty-three (64.6%) patients underwent conservative surgery, with 48 detorsions and cystectomies, 2 detorsions and cyst fenestrations, 1 detorsion only and 2 salpingectomies only. Twenty-nine (25.4%) patients underwent unilateral salpingo-oophorectomy. There were no cases of postoperative thrombosis, spontaneous abortion or recurrence. Seven patients underwent simultaneous artificial abortion. One patient experienced intrauterine fetal death, and 74 patients had live births.Conclusion Surgical intervention was required as soon as possible. Laparoscopic conservative surgery is safe and may be appropriate to preserve ovarian function. Cystectomy could reduce the risk of recurrence.
The Relationship Between Lipid Profile and Outcomes of Pregnant Women with Gestational Diabetes Mellitus and Hypertensive Disorders: A Retrospective Study
Authors: Li Yuan , Chen Wenqing , Wang Xiaoqian , Jiang Fengjuan , Chen Xiaotian 
Published: June 2020

Abstract
Background Dyslipidemia during pregnancy in women with gestational diabetes mellitus (GDM) and hypertensive disorders of pregnancy (HDP) has been extensively studied in western countries. However, similar researches have rarely been conducted in Asia. The present study aimed to evaluate maternal lipid profile changes in relation to pregnancy complications and clinical outcomes.Methods The medical records of 668 pregnant women were retrospectively analyzed. Maternal fasting serum lipids were assayed for total cholesterol (TC), triglycerides (TG), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), apolipoprotein A1 (Apo A1) and Apo B concentrations during the second trimesters. The atherogenic index of plasma (AIP) was calculated. The pregnancy complications included GDM and HDP. The clinical outcomes were collected by evaluating delivery mode, postpartum hemorrhage, macrosomia, birth weight, body length and neonatal Apgar score.Results Levels of TG and AIP were elevated while decreased TC and HDL-C was observed in women with GDM compared with that of the control group (p < 0.05). TG and AIP in HDP group were higher than in control group (p < 0.05). Significant differences were observed in postpartum hemorrhage, birth weight and body length between control group and women with GDM or HDP (p < 0.05). Compared with women with serum TG ≥ 2.5 mmol/L or TC ≥ 6.2 mmol/L, the incidence of GDM, cesarean section and macrosomia was lower in normal lipid group (p < 0.05). Spearman correlation analysis showed that the incidence of GDM was positively correlated with levels of TG (r = 0.226, p < 0.01) and AIP (r = 0.250, p < 0.01), while negative correlated with TC (r = -0.106, p < 0.01) and HDL-C (r = -0.190, p < 0.01). AIP was correlated positively with the incidence of HDP (r = 0.115, p = 0.043). According to the logistic regression analysis, each unit elevation in AIP increased the risk of GDM by 6.943 times (OR = 6.943, 95% CI: 1.761–27.374).Conclusions Lipid control, especially for women with GDM and HDP, might be a potential strategy for improving the pregnancy outcomes.