bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laboratory Evaluation of Egg White and Milk External Biomarkers for Wasmannia auropunctata (Hymenoptera: Formicidae)
Authors: Montgomery Michelle , Hagler James R , Vanderwoude Casper , Lynch A Jasmyn J , Robinson Wayne A 
Published: December 2019

Abstract
Acquisition and retention of two protein markers were tested on little fire ants, Wasmannia auropunctata Roger. Pure (100%) cow’s milk and a dilution (10%) of chicken egg whites were applied to W. auropunctata directly by contact spray plus residue or indirectly via residual contact only with protein-marked plant debris. Protein-marked ants were held in plastic shoe-box-sized containers, collected at 0, 24, and 48 h after exposure to their respective marks, and then examined for the presence of the marks by a chicken egg albumin and milk casein-specific enzyme-linked immunosorbent assay. Cross-contamination rates were assessed by allowing ants marked with egg whites to interact with an equal number marked milk for 24 and 48 h, and then collected either individually or in bulk. Results indicated that the egg white biomarker was retained longer than milk and that more ants were successfully marked when the direct spray application method was employed. Cross-contamination rates were highest among bulk-collected ants and lowest among ants collected individually after 24 h. However, the rates of cross-contamination among individually collected ants increased and were similar to that of bulk-collected ants after 48 h. On the basis of our results, external protein marking may not be suitable if mass trapping is required or if the study extends beyond 24 h due to high cross-contamination rates among specimens collected in bulk and reduced marker detection rates.
Traveling to thermal refuges during stressful temperatures leads to foraging constraints in a central-place forager
Authors: Haase Catherine G , Fletcher Robert J , Slone Daniel H , Reid James P , Butler Susan M 
Published: December 2019

AbstractCentral-place foragers can be constrained by the distance between habitats. When an organism relies on a central place for thermal refuge, the distance to food resources can potentially constrain foraging behavior. We investigated the effect of distance between thermal refuges and forage patches of the cold-intolerant marine mammal, the Florida manatee (Trichechus manatus latirostris), on foraging duration. We tested the alternative hypotheses of time minimization and energy maximization as a response to distance between habitats. We also determined if manatees mitigate foraging constraints with increased visits to closer thermal refuges. We used hidden Markov models to assign discrete behaviors from movement parameters as a function of water temperature and assessed the influence of distance on foraging duration in water temperatures above (&gt; 20°C) and below (≤ 20°C) the lower critical limit of the thermoneutral zone of manatees. We found that with increased distance, manatees decreased foraging duration in cold water temperature and increased foraging duration in warmer temperatures. We also found that manatees returned to closer thermal refuges more often. Our results suggest that the spatial relationship of thermal and forage habitats can impact behavioral decisions regarding foraging. Addressing foraging behavior questions while considering thermoregulatory behavior implicates the importance of understanding changing environments on animal behavior, particularly in the face of current global change.
Quantitation of Cell-Derived Microparticles in Blood Products and Its Potential Applications in Transfusion Laboratories
Authors: Noulsri Egarit 
Published: December 2019

AbstractCell-derived microparticles (MPs) are small fragments released from various cells when they are activated or undergo apoptosis. In the field of transfusion medicine, a number of studies have documented increased levels of MPs in blood products, which have been associated with multiple factors, including donor variability, blood component processing, and storage. In addition, transfusions that contain high levels of MPs are linked to posttransfusion complications. Considering the clinical importance of MP levels, transfusion laboratories should routinely screen blood products for them. However, this practice is not yet applied routinely, perhaps in part because of a lack of understanding of how to apply MP data to transfusion medicine. We describe the methods used to quantitate MPs in blood components and discuss the application of these quantitative data in routine transfusion laboratories in order to manage quality, improve the outcomes of transfusions, and minimize their complications.
Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics
Authors: Song Jinzhao , Hegge Jorrit W , Mauk Michael G , Chen Junman , Till Jacob E , Bhagwat Neha , Azink Lotte T , Peng Jing , Sen Moen , Mays Jazmine , Carpenter Erica L , van der Oost John , Bau Haim H 
Published: December 2019

Abstract
Detection of disease-associated, cell-free nucleic acids in body fluids enables early diagnostics, genotyping and personalized therapy, but is challenged by the low concentrations of clinically significant nucleic acids and their sequence homology with abundant wild-type nucleic acids. We describe a novel approach, dubbed NAVIGATER, for increasing the fractions of Nucleic Acids of clinical interest Via DNA-Guided Argonaute from Thermus thermophilus (TtAgo). TtAgo cleaves specifically guide-complementary DNA and RNA with single nucleotide precision, greatly increasing the fractions of rare alleles and, enhancing the sensitivity of downstream detection methods such as ddPCR, sequencing, and clamped enzymatic amplification. We demonstrated 60-fold enrichment of the cancer biomarker KRAS G12D and ∼100-fold increased sensitivity of Peptide Nucleic Acid (PNA) and Xenonucleic Acid (XNA) clamp PCR, enabling detection of low-frequency (&lt;0.01%) mutant alleles (∼1 copy) in blood samples of pancreatic cancer patients. NAVIGATER surpasses Cas9-based assays (e.g. DASH, Depletion of Abundant Sequences by Hybridization), identifying more mutation-positive samples when combined with XNA-PCR. Moreover, TtAgo does not require targets to contain any specific protospacer-adjacent motifs (PAM); is a multi-turnover enzyme; cleaves ssDNA, dsDNA and RNA targets in a single assay; and operates at elevated temperatures, providing high selectivity and compatibility with polymerases.
FluoroCalins: engineered lipocalins with novel binding functions fused to a fluorescent protein for applications in biomolecular imaging and detection
Authors: Eggenstein Evelyn , Richter Antonia , Skerra Arne 
Published: December 2019

AbstractFluoroCalins represent novel bifunctional protein reagents derived from engineered lipocalins fused to a fluorescent reporter protein, here the enhanced green fluorescent protein (eGFP). We demonstrate the construction, facile bacterial production and broad applicability of FluoroCalins using two Anticalin® molecules directed against the tumor vasculature-associated extra domain B of fibronectin (ED-B) and the vascular endothelial growth factor receptor 3, a marker of tumor and lymphangiogenesis. FluoroCalins were prepared with two different spacers: (i) a short Ser3Ala linker and (ii) a long hydrophilic and conformationally unstructured PASylation® polypeptide comprising 200 Pro, Ala and Ser residues. These FluoroCalins were applied for direct target quantification in enzyme-linked immunosorbent assay as well as target detection by flow cytometry and fluorescence microscopy of live and fixed cells, respectively, demonstrating high specificity and signal-to-noise ratio. Hence, FluoroCalins offer a promising alternative to antibody-based reagents for state of the art fluorescent in vitro detection and biomolecular imaging.
Identification of novel dynamin‐related protein 1 (Drp1) GTPase inhibitors: Therapeutic potential of Drpitor1 and Drpitor1a in cancer and cardiac ischemia‐reperfusion injury
Authors: Wu Danchen , Dasgupta Asish , Chen Kuang‐Hueih , Neuber‐Hess Monica , Patel Jignesh , Hurst Timothy E. , Mewburn Jeffrey D. , Lima Patricia D. A. , Alizadeh Elahe , Martin Ashley , Wells Michael , Snieckus Victor , Archer Stephen L. 
Published: December 2019

AbstractMitochondrial fission is important in physiological processes, including coordination of mitochondrial and nuclear division during mitosis, and pathologic processes, such as the production of reactive oxygen species (ROS) during cardiac ischemia‐reperfusion injury (IR). Mitochondrial fission is mainly mediated by dynamin‐related protein 1 (Drp1), a large GTPase. The GTPase activity of Drp1 is essential for its fissogenic activity. Therefore, we aimed to identify Drp1 inhibitors and evaluate their anti‐neoplastic and cardioprotective properties in five cancer cell lines (A549, SK‐MES‐1, SK‐LU‐1, SW 900, and MCF7) and an experimental cardiac IR injury model. Virtual screening of a chemical library revealed 17 compounds with high predicted affinity to the GTPase domain of Drp1. In silico screening identified an ellipticine compound, Drpitor1, as a putative, potent Drp1 inhibitor. We also synthesized a congener of Drpitor1 to remove the methoxymethyl group and reduce hydrolytic lability (Drpitor1a). Drpitor1 and Drpitor1a inhibited the GTPase activity of Drp1 without inhibiting the GTPase of dynamin 1. Drpitor1 and Drpitor1a have greater potency than the current standard Drp1 GTPase inhibitor, mdivi‐1, (IC50 for mitochondrial fragmentation are 0.09, 0.06, and 10 μM, respectively). Both Drpitors reduced proliferation and induced apoptosis in cancer cells. Drpitor1a suppressed lung cancer tumor growth in a mouse xenograft model. Drpitor1a also inhibited mitochondrial ROS production, prevented mitochondrial fission, and improved right ventricular diastolic dysfunction during IR injury. In conclusion, Drpitors are useful tools for understanding mitochondrial dynamics and have therapeutic potential in treating cancer and cardiac IR injury.
SUMO1 SUMOylates and SENP3 deSUMOylates NLRP3 to orchestrate the inflammasome activation
Authors: Shao Luyao , Liu Yan , Wang Wenbiao , Li Aixin , Wan Pin , Liu Weiyong , Shereen Muhammad Adnan , Liu Fang , Zhang Wen , Tan Quiping , Wu Kailang , Liu Yingle , Wu Jianguo 
Published: December 2019

AbstractThe NLRP3 inflammasome regulates innate immune and inflammatory responses by promoting caspase1‐dependent induction of pro‐inflammatory cytokines. However, aberrant inflammasome activation causes diverse diseases, and thus inflammasome activity must be tightly controlled. Here, we reveal a molecular mechanism underlying the regulation of NLRP3 inflammasome. NLRP3 interacts with SUMO‐conjugating enzyme (UBC9), which subsequently promotes small ubiquitin‐like modifier 1 (SUMO1) to catalyze NLRP3 SUMOylation at residue Lys204. SUMO1‐catalyzed SUMOylation of NLRP3 facilitates ASC oligomerization, inflammasome activation, and interleukin‐1β secretion. Moreover, this study also reveals that SUMO‐specific protease 3 (SENP3) is required for the deSUMOylation of NLRP3. Interestingly, SENP3 deSUMOylates NLRP3 to attenuate ASC recruitment and speck formation, the NLRP3 inflammasome activation, as well as IL‐1β cleavage and secretion. In conclusion, we reveal that SUMO1‐catalyzed SUMOylation and SENP3‐mediated deSUMOylation of NLRP3 orchestrate the inflammasome activation.
Implementing a Rehabilitation Protocol for Spatial Neglect Assessment and Treatment in an Acute Care Hospital
Authors: Hreha Kimberly , Chen Peii , LaRosa Jennifer , Santos Christopher , Gocon Cindy , Barrett A.M. 
Published: December 2019

Purpose:
Spatial neglect can occur after stroke, causing disabling spatial errors limiting functional abilities. In rehabilitation research, administration of spatial neglect assessment and treatment protocols can improve function. The objective of this study was to implement those protocols, the Kessler Foundation Neglect Assessment Process (KF-NAP) and the Kessler Foundation Prism Adaptation Treatment (KF-PAT), in an acute care hospital to determine their feasibility and sustainability.

Methods:
We formally instructed participating therapists, followed by a 5-month training phase and then 6-month implementation phase. Patient participants included right brain stroke survivors, at acute care. We measured patient-based (study participation rates, refusals) and institutional-based feasibility (fidelity and maintenance) for both protocols.

Results:
A total of 126 of 145 patients with stroke meeting the inclusion criteria were assessed for spatial neglect. Among the 126, 20.6% had spatial neglect. Ten of the 26 patients with spatial neglect were treated with the KF-PAT. There were no refusals; however, 3 people were not seen because of medical scheduling. Overall, the fidelity of both protocols was maintained because main concepts and components were not changed. However, adaptations to both protocols were required secondary to lack of time and medical complexity of the patients.

Conclusions:
Implementation of a standard rehabilitation assessment and treatment protocol for spatial neglect in acute care is feasible. The findings related to fidelity are promising; however, further research is recommended.
A Survey Study of Health Promotion, Wellness, and Prevention in Wound Management Environments
Authors: Wendland Deborah M. , Taylor David W. M. , Lucado Ann M. 
Published: December 2019

Purpose:
To survey physical therapists and physical therapist assistants practicing in wound management environments regarding their personal beliefs, clinical practices, and perceived barriers to incorporating health promotion, wellness, and prevention (HPWP) in practice.

Methods:
A 30-question validated survey was sent to wound care therapists.

Results:
The qualified response rate was 29%. Therapists reported a belief in their professional role in HPWP as follows: physical (97%), psychological (82%), emotional (79%), intellectual (73%), occupational (73%), social (71%), and spiritual (55%). Practice behaviors that were frequently included by these individuals included nutrition optimization (88%), diabetes/metabolic syndrome education (81%), physical activity/fitness prescription (81%), and tobacco cessation (78%). Conditions least addressed included violence (91%) and substance-free living (75%). Common barriers included lack of resources, time, patient interest, and economic limitations of patients. Respondent lifestyles were generally healthier than adults in the United States.

Conclusions:
There is an increasing need in physical therapist practice to actively promote health and wellness at the level of the individual. Modeling health behaviors is important to include as well and may be beneficial to support patient healthy lifestyle. While wound management therapists do well in promoting health in certain areas, work is still needed in others.
Demographic Factors of Nasal Bone Fractures and Social Reflection
Authors: Byun Il Hwan , Lee Won Jai , Roh Tai Suk , Hong Jong Won 
Published: December 2019

Introduction:
The nose is the most protruding central part of the face, and nasal bone fractures are the most common of facial bone fractures, leading up to 39%. Despite its high frequency, not many studies handled the etiology of nasal bone fractures, leading to the necessity of a recent demographic study.

Materials and Methods:
A total of 1111 patients diagnosed with nasal bone fracture from 2013 to 2018 at our institute were evaluated. A retrospective review of the various demographic and etiologic characteristics was done.

Results:
Numerous factors associated with nasal bone fractures were analyzed. Male patients were 3.3 times greater than the number of female patients, and twenties were the most common age range. Violence was the overall most common cause of injury, while slip down was the most common cause for females. Upon monthly distribution, the highest incidence of nasal bone fractures occurred in September, followed by March and December. The authors further divided the patients by time zone of the fractures, and male patients were most commonly injured from midnight to 3 AM, while female patients were 6 PM to 9 PM.

Conclusion:
Our findings represent a recent urban data of various etiologic factors of nasal bone fracture. The concept of school violence has been widely used since 1990s, and our data reflects that violence of the youth is a serious issue of the society. The mechanism of injury differed among sexes and age groups, and such discrepancies will aid physicians to better understand facial bone fracture patients and educate them in the future.
Liquid-like protein interactions catalyze assembly of endocytic vesicles
Authors: Day Kasey J. , Kago Grace , Wang Liping , Richter J Blair , Hayden Carl C. , Lafer Eileen M. , Stachowiak Jeanne C. 
Published: December 2019

AbstractDuring clathrin-mediated endocytosis, dozens of proteins assemble into an interconnected network at the plasma membrane. As early initiators of endocytosis, Eps15 and Fcho1 are responsible for locally concentrating downstream components on the membrane surface. However, they must also permit dynamic rearrangement of proteins within the budding vesicle. How do initiator proteins meet these competing demands? Here we show that Eps15 and Fcho1 rely on weak, liquid-like interactions to efficiently catalyze endocytosis. In reconstitution experiments, these weak interactions promote the assembly of protein droplets with liquid-like properties, including rapid coalescence and dynamic exchange of protein components. To probe the physiological role of liquid-like interactions among initiator proteins, we tuned the strength of initiator protein assembly in real time using light-inducible oligomerization of Eps15. Low light levels drove initiator proteins into liquid-like assemblies, restoring normal rates of endocytosis in mammalian Eps15 knockout cells. In contrast, initiator proteins formed solid-like assemblies upon exposure to higher light levels. Assembly of these structures stalled vesicle budding, likely owing to insufficient molecular rearrangement. These findings suggest that liquid-like assembly of early initiator proteins provides an optimal catalytic platform for endocytosis.
Chromosome Evolution of Octoploid Strawberry
Authors: Hardigan Michael A. , Feldmann Mitchell J. , Lorant Anne , Famula Randi , Acharya Charlotte , Cole Glenn , Edger Patrick P. , Knapp Steven J. 
Published: December 2019

AbstractThe allo-octoploid cultivated strawberry (Fragaria×ananassa) originated through a combination of polyploid and homoploid hybridization, domestication of an interspecific hybrid lineage, and continued admixture of wild species over the last 300 years. While genes appear to flow freely between the octoploid progenitors, the genome structures and diversity of the octoploid species remain poorly understood. The complexity and absence of an octoploid genome frustrated early efforts to study chromosome evolution, resolve subgenomic structure, and develop a single coherent linkage group nomenclature. Here, we show that octoploidFragariaspecies harbor millions of subgenome-specific DNA variants. Their diversity was sufficient to distinguish duplicated (homoeologous and paralogous) DNA sequences and develop 50K and 850K SNP genotyping arrays populated with co-dominant, disomic SNP markers distributed throughout the octoploid genome. Whole-genome shotgun genotyping of an interspecific segregating population yielded 1.9M genetically mapped subgenome variants in 5,521 haploblocks spanning 3,394 cM inF. chiloensissubsp.lucida, and 1.6M genetically mapped subgenome variants in 3,179 haploblocks spanning 2,017 cM inF. ×ananassa. These studies provide a dense genomic framework of subgenome-specific DNA markers for seamlessly cross-referencing genetic and physical mapping information, and unifying existing chromosome nomenclatures. Through comparative genetic mapping, we show that the genomes of geographically diverse wild octoploids are effectively diploidized and completely collinear. The preservation of genome structure among allo-octoploid taxa is a critical factor in the unique history of garden strawberry, where unimpeded gene flow supported both its origin and domestication through repeated cycles of interspecific hybridization.
How does management innovation matter for performance: Efficiency or legitimacy?
Authors: Wei Zelong , Song Xi , Xie Paike 
Published: December 2019

PurposeDespite increased research attention to management innovation, the literature offers conflicting explanations of how it affects firm performance. The rational perspective emphasises the role of management innovation for organisational routine updating. The fashion perspective views management innovation as a symbolic activity to foster legitimacy. The purpose of this study is to integrate the two perspectives and explore both the mediating effects of organisational efficiency and business legitimacy and the moderating effect of CEO shareholding.Design/methodology/approachBased on empirical data from 238 Chinese firms, this study conducts stepwise regression to test the hypotheses.FindingsThis study finds that management innovation positively affects both organisational efficiency and business legitimacy and then firm performance. However, the promotion effect of organisational efficiency is stronger than that of business legitimacy on firm performance. The results further indicate that CEO shareholding strengthens the effect of management innovation on organisational efficiency but weakens it for business legitimacy.Originality/valueThis study presents a complete explanation of the effect of management innovation on firm performance by exploring the mediating effect of both organisational efficiency and business legitimacy. Further, it compares the effects of organisational efficiency and business legitimacy on firm performance. Finally, it resolves the conflict between the rational and fashion perspectives by involving the moderating effect of CEO shareholding.
¿Qué hacer cuando tú marca es secuestrada por Narcos? El caso del whisky de Buchanan’s
Authors: Kervyn Nicolas , Cavazos Arroyo Judith , Castillo Villar Fernando Rey , Gomez Zuñiga Rosa Andrea 
Published: December 2019

Learning outcomes
Entender la diferencia entre identidad de marca e imagen de marca. - Aplicar varias herramientas de segmentación - Entender el atractivo aspiracional de la marca y su consecuencia en el consumo privado y público. - Explorar las opciones estratégicas disponibles para una marca que enfrenta una apropiación de marca. - Explorar los pros y los contras de oponerse a una apropiación de marca. Desarrollar un plan para la implementación de esta estrategia - Explorar los pros y los contras de utilizar la estrategia de Laisser-Faire en la apropiación de la marca. Desarrollar un plan para la implementación de esta estrategia - Explorar los pros y los contras de instrumentalizar la apropiación de marca. Desarrollar un plan para la implementación de esta estrategia

Case overview/synopsis
Este caso pretende ayudar a los alumnos a comprender la diferencia entre la identidad de marca (la que los propietarios de estas pretenden que se perciba) y la imagen de marca (la que los consumidores perciben realmente).

Complexity academic level
Este caso está diseñado para ser utilizado en un curso de gestión de marketing, estrategias de marca o cultura del consumidor. Especificamente, el caso esta diseñado para estudiantes de último año de licenciatura o maestría en Mercadotecnia ya que tienen nociones básicas de mercadotecnia estratégica. Debería proporcionar bases para las discusiones sobre temas de gestión de marca, cultura del consumidor, gestión de carteras de marca, marketing internacional, estrategia de reposicionamiento, arquitectura de marca, el valor de la marca, los valores de la marca, apropiación de la marca y relaciones de los consumidores con estas.

Supplementary materials
Teaching Notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes.

Subject code
CSS 8: Marketing.