bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Early Detection of Neuroendocrine Prostate Cancer as Lineage Plasticity in Recurrent Prostate Cancer After Radiation and Androgen Deprivation Therapy: A Case Report
Authors: Komori Takahiro , Kosaka Takeo , Watanabe Keitaro , Yasumizu Yota , Mikami Shuji , Oya Mototsugu 
Published: November 2020

Abstract
Background: Neuroendocrine prostate cancer is one of the most aggressive prostate cancers, with severely poor prognosis. However, its detection is difficult because no useful marker has been found so far. In addition, serum prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSMA), and PSMA positron emission tomography/computed tomography don’t help in neuroendocrine prostate cancer. However, its early detection is necessary because its prognosis is poor.Case presentation: We described three cases of early neuroendocrine prostate cancer detection after initial external beam radiotherapy followed by salvage androgen deprivation therapy (ADT). We used Magnetic Resonance Imaging for three ADT-resistant patients, and it detected neuroendocrine prostate cancer in all three, although the PSA level was <2 ng/mL.Conclusions: Magnetic resonance imaging might be a better modality for neuroendocrine prostate cancer detection despite low serum prostate-specific-antigen levels.. Our findings in these three cases will help establish better criteria or better follow-up for patients administered salvage androgen deprivation therapy for biochemical recurrence of prostate cancer after external beam radiotherapy.
Whole-exome Sequencing Reveals the Etiology of the Rare Primary Hepatic Mucoepidermoid Carcinoma
Authors: Hou Ping , Su Xiaoyan , Cao Wei , Xu Liping , Zhang Rongguiyi , Huang Zhihao , Wang Jiakun , Li Lixiang , Wu Linquan , Liao Wenjun 
Published: November 2020

Abstract
Background Primary hepatic mucoepidermoid carcinoma (HMEC)is extremely rare and the molecular etiology is still unknown.Recently, The CRTC1-MAML2 fusion gene was detected in a primary HMEC which is often associated with MEC of salivary gland in the literature. Methods a 64-year-old male was diagnosed with HMEC based on malignant squamous cells and mucus-secreting cells in immunohistochemical examination. Whole-exome sequencing(WES) and sanger sequencing were used to reveal the molecular characteristics of HMEC,and analysis with public datas among hepatocellular carcinoma, cholangiocarcinoma and salivary MEC. Meanwhile, The susceptibility genes were identified in pedigree investigation.Result Significant somatic mutations in GNAS,KMT2C,ELF3 genes were identified in primary HMEC by WES and sanger sequencing. Meanwhile, through public data analysis, somtatic GNAS gene alterd in 2.1% hepatobiliary tumors, and typically GNAS occur at exon 8, in which Arg201 is converted to either a cysteine (R201C) or a histidine (R201H) related with cholangiocarcinoma associated with parasite infection .Furthermore, heterozygous germline mutations of FANCA, FANCI, FANCJ/BRIP1 and FAN1 genes were also identified. Pedigree investigation verified that mutation of susceptibility genes of Fanconi's anemia were present in the pedigree.Conclusions It was the first time to demonstrate the molecular etiology of the rare HMEC associated with germline Fanconi’s anemia mutations and somatic GNAS R201H mutation.
Adipocytokine Profile Reveals a Significant Cytokine Network in the Acute Phase of Sepsis
Authors: Ebihara Takeshi , Matsumoto Hisatake , Matsubara Tsunehiro , Matsuura Hiroshi , Hirose Tomoya , Shimizu Kentaro , Ogura Hiroshi , Kang Sujin , Tanaka Toshio , Shimazu Takeshi 
Published: November 2020

Abstract
Background: The cytokines compose a network and play a crucial role in the pathogenesis and prognosis of sepsis. Adipose tissue is noted as an important immune endocrine organ that releases adipocytokines. The aim of this study was to evaluate the adipocytokines in sepsis from a network perspective.Methods: This retrospective study comprising 37 patients with sepsis and 12 healthy controls was conducted from February 2014 to July 2015. Blood samples (maximum of 7 samples per patient, 185 samples in total) were collected from the patients on days 1 (within 24 hours of the diagnosis), 2, 4, 6, 8, 11 and 15 and from the healthy controls. Adipocytokines (adiponectin, leptin, resistin, chemerin, visfatin, vaspin, CXCL-12/SDF-1, angiotensinogen), inflammatory cytokines (IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12/IL-23p40, TNF-α, MCP-1), and PAI-1 were measured. The APACHE II score was evaluated on day 1, and the SOFA score and JAAM and ISTH overt disseminated intravascular coagulation (DIC) scores were assessed at the same time points at which blood was sampled.Results: Hierarchical clustering analysis showed that the cluster formed by resistin, IL-6, IL-8, MCP-1, and IL-10 on days 1, 2 and 4 represented the cytokine network throughout the acute phase of sepsis. There was a significant association of each cytokine in this network with SOFA and JAAM DIC scores over the acute phase. In a Cox proportional hazards model focusing on the acute phase, each of these cytokines showed a significant relation with patient prognosis.Conclusions: Adipocytokines and an inflammatory cytokine profile were assessed over time in patients with sepsis. We found that resistin was involved in an inflammatory cytokine network including IL-6, IL-8, IL-10 and MCP-1 in the acute phase of sepsis, and this network was associated with the severity and prognosis of sepsis.
Prevalence of IgG Antibodies to SARS-CoV-2 in Wuhan – Implications for the Longevity of Antibodies Against SARS-CoV-2
Authors: Liu Tao , Wu Sanyun , Tao Huangheng , Zeng Guang , Zhou Fuling , Wang Xinghuan 
Published: November 2020

Abstract
Background It is to be determined whether people infected with SARS-CoV-2 will develop long-term immunity against SARS-CoV-2 and retain long-lasting antibodies after the infection is resolved. This study was to explore the outcomes of IgG antibodies to SARS-CoV-2 in four groups of individuals in Wuhan, China.MethodsWe conducted a cross-sectional study on the following four groups who received both COVID-19 IgM/IgG tests and RT-PCR tests for SARS-CoV-2 from February 29, 2020 to April 29, 2020: 1470 hospitalized patients with COVID-19 from Leishenshan Hospital, Zhongnan Hospital of Wuhan University, and Wuhan No. 7 Hospital, 3832 healthcare providers without COVID-19 diagnosis, 19555 general workers, and 1616 other patients to be admitted to the hospital (N=26473). COVID-19 patients who received IgM/IgG tests <21 days after symptom onset were excluded. Results IgG prevalence was 89.8% (95% CI 88.2-91.3%) in COVID-19 patients, 4.0% (95% CI 3.4-4.7%) in healthcare providers, 4.6 (95% CI 4.3-4.9 %) in general workers, and 1.0% in other patients (p all <0.001 for comparisons with COVID-19 patients). IgG prevalence increased significantly by age among healthcare workers and general workers. Among hospitalized COVID-19 patients, presence of IgG antibodies to SARS-CoV-2 was not associated with most disease severity, presence of comorbidities, treatment received, and clinical characteristics. We identified 24 hospitalized patients with COVID-19 and multiple COVID-19 antibody tests who lost previously detected IgG antibodies to SARS-CoV-2ConclusionsIgG antibodies to SARS-CoV-2 in infected people may become undetectable overtime.
Human Umbilical Cord Mesenchymal Stem Cell-derived Exosomal miR-27b Attenuate Subretinal Fibrosis via Suppressing Epithelial-mesenchymal Transition by Targeting HOXC6
Authors: Li dongli , Zhang Junxiu , Liu Zijia , Gong Yuanyuan , Zheng Zhi 
Published: November 2020

Abstract
Background and aimSubretinal fibrosis resulting from neovascular age-related macular degeneration (nAMD) is one of the major causes of serious and irreversible vision loss worldwide, and no definite and effective treatment exists currently. Retinal pigmented epithelium (RPE) cells are crucial in maintaining the visual function of normal eyes and its epithelial–mesenchymal transition (EMT) is associated with the pathogenesis of subretinal fibrosis. Stem cells-derived exosomes have been reported to play a crucial part in tissue fibrosis by transferring their molecular contents. This study aimed to explore the effects of human umbilical cordderived mesenchymal stem cell exosomes (hucMSC-Exo) on subretinal fibrosis in vivo and in vitro and to investigate the anti-fibrotic mechanism of hucMSC-Exo.Methods In this study, we successfully cultured and identified human umbilical cord-derived mesenchymal stem cells (hucMSC), and isolated exosomes from their supernatant by ultracentrifugation.Laser-induced (choroidal neovascularization) CNV and subretinal fibrosis model indicated that intravitreal administration of hucMSC-Exo effectively alleviated subretinal fibrosis in vivo. Furthermore, we found that hucMSC-Exo could efficaciously suppress RPE cells migration and promote the mesenchymal–epithelial transition (MET) by delivering miR-27b-3p. Analysis of the latent binding of miR-27b-3p to HOXC6 was made by bioinformatics prediction and luciferase reporter assays. ResultsThe study showed that intravitreal injection of hucMSC-Exo effectively ameliorated laser-induced CNV and subretinal fibrosis via suppression of EMT process. In addition, hucMSC-Exo containing miR-27b repressed the EMT process in RPE cells induced by the TGF-β2 via inhibiting HOXC6 (Homeobox protein Hox-C6) expression. ConclusionsThis study provided novel insights into the anti-fibrotic mechanism of hucMSC-Exo on subretinal fibrosis. HucMSCs-derived exosomal miR-27b could reverse the process of EMT induced by TGF-β2 via inhibiting HOXC6, which indicated that exosomal miR-27b/HOXC6 axis could play a vital role on ameliorating subretinal fibrosis. Our study put forward a promising therapeutic agent for the treatment of ocular fibrotic diseases, as well as comprehension into the mechanism of hucMSC-Exo under subretinal fibrosis.
Exosomes From LPS-stimulated hDPSCs&nbsp;Promote the Angiogenesis of HUVECs
Authors: Huang Xiangyu , Qiu Wei , Pan Yuhua , Li Jianjia , Chen Zhao , Zhang Kaiying , Luo Yifei , Wu Buling , Xv Wenan 
Published: November 2020

Abstract
Background: Angiogenesis is fundamental to biomimetic pulp regeneration. Extracellular vesicles (EVs) derived from human dental pulp stem cells (hDPSCs) from patients with periodontitis were reported to have better angiogenic capabilities. However, the underlying regulatory mechanism remains unknown. As an important component of EVs, exosomes from hDPSCs were indicated to play a crucial role in multiple regeneration processes. In this study, the inflammatory factor lipopolysaccharide (LPS) was used to stimulate hDPSCs, and exosomes were extracted from these hDPSCs. The role of exosomes in the angiogenesis of Human Umbilical Vein Endothelial Cells (HUVECs) was examined, and the underlying mechanism was studied.Method: Exosomes were isolated from hDPSCs with or without LPS stimulation. The angiogenic capabilities of HUVECs were evaluated after coculture with exosomes derived from hDPSCs (hDPSC-EXOs) or exosomes derived from LPS-stimulated hDPSCs (LPS-hDPSC-EXOs). Tube formation and migration assays were conducted, and angiogenesis-related mRNA expression was detected. MicroRNA sequencing was performed to explore the microRNA profile of hDPSC-EXOs and LPS-hDPSC-EXOs. Gene Ontology (GO) analysis was used to study the functions of the predicted target genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to estimate the signaling pathways associated with the inflammation-induced angiogenesis process.Result: The release of hDPSC-EXOs increased after stimulation with LPS. Compared to endocytosis of hDPSC-EXOs, endocytosis of LPS-hDPSC-EXOs promoted the tube formation and migration of HUVECs. The mRNA expression levels of vascular endothelial growth factor (VEGF) and kinase-insert domain-containing receptor (KDR) in the LPS-hDPSC-EXOs group were significantly higher than those in the hDPSC-EXOs group. MicroRNA sequencing showed that 10 microRNAs were significantly changed in LPS-hDPSC-EXOs; of these microRNAs, 7 were increased, and 3 were decreased. Pathway analysis showed that the genes targeted by differentially expressed microRNAs were involved in multiple angiogenesis-related pathways.Conclusion: This study revealed that exosomes derived from inflammatory hDPSCs displayed a stronger effect on vascular regeneration. It’s the first time to explore the role of exosomal microRNA from hDPSCs in inflammation-induced angiogenesis. This finding sheds new light on the effect of inflammation-stimulated hDPSCs on tissue regeneration.
The value of neurohumoral regulation in the outcome of multiple organ failure syndrome in sepsis
Authors: Leontiev M. A. , Vodova A. B. , Kravchuk S. V. 
Published: November 2020

The objective: to present information about potential mechanisms of development of sepsis-associated encephalopathy, and its potential role in sepsis outcome.Neurohumoral regulation is the most important system that integrates many functions of variable values to achieve the final result that is beneficial for the host. The central nervous system (CNS) is the switch and control mechanism responsible for the functioning of this system. The increasing number of studies indicating the relationship between the development of sepsis and occurrence of qualitative and quantitative changes in the central nervous system suggests that it is the degree of damage to neurohumoral regulation mechanisms at the very beginning of the disease can significantly determine the severity of the course and prognosis of the outcome of multiple organ failure syndrome in sepsis.
Pembelajaran Keaksaraan Dasar PKBM Bina Sekar Melati di Desa Triharjo, Kecamatan Pandak, Bantul
Authors: Sutrisno Sutrisno 
Published: November 2020

AbstrakPenelitian ini bertujuan untuk mengetahui (1) Pelaksanaan pendidikan keaksaraan dasar, (2) Hasil dari pendidikan keaksaraan dasar, dan (3) Faktor-faktor pendukung dan penghambat dalam pelaksanaaan pendidikan keaksaraan dasar. Penelitian ini menggunakan metode penelitian kualitatif dengan pendekatan deskriptif kualitatif. Subjek penelitian ini adalah pengelola PKBM Bina Sekar Melati, tutor pendidikan keaksaraan dan warga belajar pendidikan keaksaraan dasar. Metode pengumpulan data yang digunakan adalah wawancara, observasi dan dokumentasi, Teknik analisis data menggunakan miles dan huberman. Hasil penelitian yaitu (1) Pelaksanaan Pendidikan keaksaraan dasar di Desa Triharjo, Kecamatan Pandak, Kabupaten Bantul berjalan dengan baik, (2) Hasil dari pendidikan keaksaraan dasar ialah warga belajar mampu membaca, menulis dan berhitung sederhana setelah diberikan pembelajaran, dan (3) Faktor pendukung pelaksanaan pendidikan keaksaraan dasar ialah (a) Kemampuan tutor, (b) dukungan dari pemerintah pusat, (c) dukungan dari pemerintah setempat, (d) motivasi dari warga belajar, dan (e) sarana dan prasarana yang memadai . Sedangkan faktor penghambatnya ialah (a) daya tangkap warga belajar berbeda-beda, (b) Kegiatan di desa seperti arisan PKK, Membantu tetangga yang mempunyai hajatan serta mati listrik membuat warga belajar tidak berangkat pembelajaran Basic Literacy Learning PKBM Bina Sekar Melati in Triharjo Village, Pandak District, Bantul AbstractThis study aims to determine (1) Implementation of basic literacy education, (2) Results of basic literacy education, and (3) Supporting and inhibiting factors in implementing basic literacy education. This study uses qualitative research methods with a qualitative descriptive approach. The subjects of this study were the manager of PKBM Bina Sekar Melati, literacy education tutors and residents learning basic literacy education. Data collection methods used were interviews, observation and documentation, data analysis techniques using miles and Huberman. The results of the study are (1) The implementation of basic literacy education in Triharjo Village, Pandak Subdistrict, Bantul Regency is going well, (2) The results of basic literacy education are the people learning to be able to read, write and count simple after learning, and (3) Factors supporters of the implementation of basic literacy education are (a) Tutor ability, (b) support from the central government, (c) support from local government, (d) motivation from learning citizens, and (e) adequate facilities and infrastructure. While the inhibiting factors are (a) the different learning capacities of the residents of learning, (b) Activities in the village such as the PKK social gathering, helping neighbors who have a celebration and power outages make learning residents not go to learning.
“Mirror of Effect” dalam Perkembangan Perilaku Anak pada Masa Pandemi Covid 19
Authors: Ismaniar Ismaniar , Utoyo Setiyo 
Published: November 2020

AbstrakWork From Home (WFH) selama masa pandemi covid 19 memberikan dampak yang cukup beragam bagi setiap orang. Terjadinya perubahan secara tiba-tiba dalam habitual keseharian senantiasa menyebabkan ketidaksiapan secara emosional pada setiap anggota keluarga, baik orang tua maupun anak. Tujuan penelitian ini adalah meningkatkan kesadaran orang tua akan pengaruh negatif yang dapat terjadi akibat interaksi yang terjadi antara anggota keluarga, pada perkembangan perilaku anak usia dini. Metode penelitian yang digunakan adalah studi literatur. Hasil penelitian menunjukkan bahwa; Mirror of effect merupakan suatu fenomena yang terjadi dimana perilaku anak mencerminkan perilaku lingkungan sosial di sekitarnya. Karakteristik anak yang cenderung mudah berimitasi, belum memiliki konsep yang kuat tentang moral serta menyukai aktivitas bermain menyebabkan kemungkinan Mirror of effect terhadap hal negatif dalam perkembangan perilakunya akan terjadi jika orang tua kurang mampu mengendalikan emosi selama Work From Home pada masa pandemi covid 19. Oleh sebab itu maka diharapkan orang tua mampu mengendalikan emosi dan selalu berusaha menciptakan lingkungan keluarga yang kondusif untuk perkembangan perilaku anak. “Mirror of Effect” in the Development of Child Behavior During Work From Home (WFH) during the Covid Pandemic 19AbstractWork From Home (WFH) during the Covid 19 pandemic had quite a variety of impacts for everyone. The sudden change in daily habituals always causes emotional unpreparedness in each family member, both parents and children. The purpose of this study is to increase parental awareness of the negative effects that can occur due to interactions that occur between family members and on the development of early childhood behavior. The research method used is literature study. The results showed that; Mirror of effect is a phenomenon that occurs in which children's behavior reflects the behavior of the social environment around them. The characteristics of children who tend to easily imitate, do not have a strong concept of morality and like playing activities, causing the possibility of a Mirror of effect on negative things in the development of their behavior if parents are less able to control emotions during Work From Home during the Covid 19 pandemic. Therefore, it is hoped that parents will be able to control emotions and always try to create a family environment that is conducive to the development of children's behavior.
The level of motor ability of soccer athletes in the FC UNY Academy in 2020
Authors: Yudanto Yudanto , Alfian Tifa 
Published: November 2020

This research aims to find out the level of motor ability of the Football Athlete at the FC UNY Academy in 2020. The purpose of this study was to determine the level of motor ability of the Football Athlete at the FC UNY Academy in 2020. This research was a quantitative research with survey method. The research population was 24 Soccer players from Yogyakarta State University FC Academy in 2020. The test instruments used in this study are using the Barrow motor ability test which includes: Standing broad jump , Soft ball throw , Zigzag run , Wall pass , Medicine ball put , Running 60 yard dash. Measuring instruments used to measure are stopwatch and meter. Data analysis techniques using descriptive analysis with percentage. The results of the research on the motor ability level of Football Athletes at the FC UNY Academy in 2020 as the following: 2 athletes (8.33%) are in very high category, 2 athletes (8.33%) are in high category, 10 athletes (41.67 %) are in moderate category, 8 athletes (33.33%) are in poor category, 2 athletes (8.33%) are in very poor category. Tingkat kemampuan motorik atlet sepakbola di Akademi FC UNY tahun 2020 AbstrakPenelitian ini bertujuan untuk mengetahui seberapa tingkat kemampuan motorik Atlet Sepakbola Di Akademi FC UNY Tahun 2020. Tujuan dari penelitian ini yaitu untuk mengetahui tingkat kemampuan motorik Atlet Sepakbola Di Akademi FC UNY Tahun 2020. Penelitian ini merupakan penelitian kuantitatif. Metode penelitian yang digunakan adalah metode survei dengan teknik tes pengukuran untuk mendapatkan data. Populasi yang digunakan dalam penelitian ini adalah Atlet Sepakbola Di Akademi FC UNY Tahun 2020 yang berjumlah 24 orang. Instrumen tes yang digunakan dalam penelitian ini yaitu menggunakan tes Barrow motor ability test yang meliputi: Standing broad jump, Soft ball throw, Zig-zag run, Wall pass, Medicine ball put, Lari 60 yard dash. Alat ukur yang digunakan untuk mengukur adalah stopwatch dan meteran. Teknik analisis data menggunakan analisis deskriptif dengan persentase. Hasil dari penelitian tingkat kemampuan motorik Atlet Sepakbola Di Akademi FC UNY Tahun 2020 sebagai berikut bahwa 2 atlet (8,33%) berada pada kategori sangat tinggi, 2 atlet (8,33%) berada pada kategori tinggi, 10 atlet (41,67%) berada pada kategori sedang, 8 atlet (33,33%) berada pada kategori kurang, 2 atlet (8,33%) berada pada kategori sangat kurang.