bluuebunny/crossref_metadata_2025_split huggingface dataset:
C3G localizes to the mother centriole in a cenexin-dependent manner and regulates centrosome duplication and primary cilium length
Authors: Nayak Sanjeev Chavan , Radha Vegesna 
Published: May 2020

ABSTRACT
C3G (also known as RAPGEF1) plays a role in cell differentiation and is essential for early embryonic development in mice. In this study, we identify C3G as a centrosomal protein that colocalizes with cenexin (also known as ODF2) at the mother centriole in interphase cells. C3G interacts with cenexin through its catalytic domain, and the two proteins show interdependence for localization to the centrosome. C3G depletion causes a decrease in cellular cenexin levels. Centrosomal localization of C3G is lost as myocytes differentiate to form myotubes. Depletion of C3G by CRISPR/Cas9 results in the formation of supernumerary centrioles, whereas overexpression of C3G, or expression of a catalytically active C3G deletion construct, inhibits centrosome duplication. Cilium length is increased in C3G knockout cells, and this phenotype is reverted upon reintroduction of C3G or its catalytic domain alone. Association of C3G with the basal body is dynamic, decreasing upon serum starvation and increasing upon re-entry into the cell cycle. C3G inhibits cilium formation and length, and this inhibition is dependent on C3G catalytic activity. We conclude that C3G regulates centrosome duplication and maintains ciliary homeostasis, properties that could be important for its role in embryonic development.
Recent Advances in treatment of urethral stricture disease in men
Authors: Mangir Naside , Chapple Christopher 
Published: May 2020

Urethral stricturing is a narrowing of the urethral lumen as a result of ischaemic spongiofibrosis. The main challenge of currently available treatment options is recurrence of the stricture. Recent advancements in the treatment of urethral strictures mainly came from the fields of regenerative medicine and tissue engineering. Research efforts have primarily focused on decreasing the recurrence of stricture after internal urethrotomy and constructing tissue-engineered urethral substitutes to improve clinical outcomes of urethroplasty surgeries. The aim of this article is to review the most recent advancements in the management of urethral stricture disease in men.
Why Isn’t Business Investment More Sensitive to Interest Rates? Evidence from Surveys
Authors: Sharpe Steven A. , Suarez Gustavo A. 
Published: May 2020

Using recent chief financial officer surveys, we examine the sensitivity of investment plans to interest rate expectations and find that, for most firms, investment is extremely insensitive to interest rate decreases and only modestly more responsive to increases. Firms cite ample cash holdings as the most frequent reason to explain their lack of sensitivity. This result is consistent with dynamic models of corporate behavior in which firms build cash buffers in anticipation of potential changes in external financing conditions. In the cross section, we find that the investment plans tend to be the least sensitive to interest rate changes at firms that do not expect to borrow over the coming year and firms facing less-binding financing constraints. This paper was accepted by Tomasz Piskorski, finance.
Robotic technology: current concepts, operative techniques and emerging uses in unicompartmental knee arthroplasty
Authors: Begum Fahima A. , Kayani Babar , Morgan Samuel D. J. , Ahmed Syed S. , Singh Sandeep , Haddad Fares S. 
Published: May 2020

Unicompartmental knee arthroplasty (UKA) is associated with improved functional outcomes but reduced implant survivorship compared to total knee arthroplasty (TKA). Surgeon-controlled errors in component positioning are the most common reason for implant failure in UKA, and low UKA case-volume is associated with poor implant survivorship and earlier time to revision surgery. Robotic UKA is associated with improved accuracy of achieving the planned femoral and tibial component positioning compared to conventional manual UKA. Robotic UKA has a learning curve of six operative cases for achieving operative times and surgical team comfort levels comparable to conventional manual UKA, but there is no learning curve effect for accuracy of implant positioning or limb alignment. Robotic UKA is associated with reduced postoperative pain, decreased opiate analgesia requirements, faster inpatient rehabilitation, and earlier time to hospital discharge compared to conventional manual UKA. Limitations of robotic UKA include high installation costs, additional radiation exposure with image-based systems, and paucity of studies showing any long-term differences in functional outcomes or implant survivorship compared to conventional manual UKA. Further clinical studies are required to establish how statistical differences in accuracy of implant positioning between conventional manual UKA and robotic UKA translate to long-term differences in functional outcomes, implant survivorship, complications, and cost-effectiveness. Cite this article: EFORT Open Rev 2020;5:312-318. DOI: 10.1302/2058-5241.5.190089
Elemental analysis of sage (herb) using calibration-free laser-induced breakdown spectroscopy
Authors: Iqbal J. , Asghar H. , Shah S. K. H. , Naeem M. , Abbasi S. A. , Ali R. 
Published: May 2020

In this work, laser-induced breakdown spectroscopy (LIBS) has been used for the quantitative and qualitative analysis of the sage sample using the calibration-free LIBS (CF-LIBS) technique. The sage plasma is generated by focusing the second harmonics (532 nm) of a

Q

-switched Nd:YAG laser with a repetition rate of 10 Hz and pulse duration of 5 ns. The emission spectra are recorded using a LIBS 2000 detection system spectrometer consisting of five high-resolution spectrometers covering a wavelength range from 200 to 720 nm. The optical emission spectra of the sage sample reveal the spectral lines of Fe, Ca, Ti, Co, Mn, Ni, and Cr. The plasma temperature and electron number density of the neutral spectral lines of the pertinent elements have been deduced using the Boltzmann plot and Stark-broadening line profile method, with average values

8855

±
885

K

and

3.89

×

10

16

c
m

−
3

, respectively. The average values of the plasma parameters were used for the quantification of the detected elements in the sample. Based on the calibration-free method, the measured results demonstrate that Fe is the major constituent in the sample, having a percentage concentration of 48.1%, while the remaining elements are Ca, Ti, Co, Mn, Ni, and Cr, with percentage concentrations 0.7%, 5.3%, 8%, 11%, 12.3%, and 14.6%, respectively. This study demonstrates the feasibility of LIBS for the compositional analysis of major and trace elements present in the plant samples and its further applications in medicine.
White-light color conversion with red/green/violet laser diodes and yellow light-emitting diode mixing for 34.8  Gbit/s visible lighting communication
Authors: Wang Wei-Chun , Cheng Chih-Hsien , Wang Huai-Yung , Lin Gong-Ru 
Published: May 2020

The visible wavelength-division multiplexing (VWDM) optical wireless communication beyond 30 Gbit/s with a white-light beam mixed by red/green/violet (R/G/V) laser diodes (LDs) and yellow (Y) LED is demonstrated via quadrature amplitude modulation discrete multitone modulation (QAM DMT). To facilitate both high-quality indoor lighting and high-speed optical wireless communication, the R/G/V-LD white-light module incorporates with a Y-LED to provide a high color rendering index (CRI) and encapsulates with a frosted glass to enlarge its divergent angle. By respectively encoding the R/G/V-LDs with the filtered QAM DMT data in a back-to-back case, the total raw data rate as high as 34.8 Gbit/s is achieved by encoded R/G/V-LDs with respective VWDM data rates of 18/7.2/9.6 Gbit/s. To fulfill the demanded CRI and correlated color temperature (CCT) for indoor white-lighting, the yellow LED contributes the yellowish-orange luminescence with flexible CCT and CRI varying from 3952 K to 3031 K and from 0 to 45.9, respectively. A cold white-light carrier at a CCT of 4852 K, CRI of 71.6, and CIE of (0.3652, 0.4942) is also approached by attenuating the red LD power, and such a cold white-light spot with an illuminance of 6800 lux and a divergent solid angle of 0.89 steradian (sr) can support VWDM data transmission at 28.4 Gbit/s.
Art as Unlearning: Towards a Mannerist Pedagogy, John Baldacchino (2019)
Authors: Vella Raphael 
Published: May 2020

Review of: Art as Unlearning: Towards a Mannerist Pedagogy, John Baldacchino (2019)
London and New York: Routledge, 166 pp.,
ISBN 978-1-13831-871-7, h/bk, £120.00
Identifikasi Permasalahan Pembelajaran Mahasiswa Tunanetra Pada Mata Kuliah Sintaksis Bahasa Inggris: Studi Kasus di Universitas Dian Nuswantoro
Authors: Sunardi Sunardi , Nugroho Raden Arief , Harjo Budi 
Published: May 2020

This study focuses on identifying the problems faced by visually impaired students in teaching-learning process of English syntax course at Faculty of Humanities, Dian Nuswantoro University in 2014. English syntax, as one of the subjects studied in the English study program, is considered as a challenging subject for visually impaired students as it involves visual perception, such as graphics and images. This research was a descriptive qualitative one. The researchers collected the data by using an in-depth interview. This involved 2 visually impaired students who experienced the learning process of English syntax and 3 lecturers of Dian Nuswantoro University. The discussion was limited on the issues around the needs for creating an accessible learning media and kinds of difficulties faced by the visually impaired students. From the in-depth interview, there are three major findings that are highlighted: a) the needs to overcome all inaccessible English syntax learning materials; b) general difficulties faced by visually impaired students in English syntax subject; c) technical difficulties in drawing tree structure diagrams. Then, these highlighted problems and findings are used as to formulate the recommendations of this study.
Relational Sociological Analysis of Uncertainties: The case of COVID-19 In Turkey
Authors: Kasapoglu Aytul , Akbal Alev 
Published: May 2020

The main research problem of this study is that due to the uncertainties experienced, rationality has been replaced by feelings like morale panic and not all of the precautions will be applied by 55%, although it is known to be 90%. The main purpose of this article, Turkey and all experienced Covidien-19 (Corono virus)from the effects of a new virus in the world, is to examine the sociological basis of the news (reports) in the Turkish media The concepts of "uncertainties", "turning point" and "liminality" in social relations and values, classified by Harrison White (1992), constitute the theoretical basis of this relational sociological study. The research questions, in which the article was sought in the critical analysis of the media, were formed on the basis of this theoretical framework. Uncertainties in social relations and values have been tried to be revealed through political discourse analysis of pictures and messages (Van Dick, 2016). Research findings revealed that the news in the media related to COVID-19virus caused attitudes and behaviors similar to those observed in previous bird flue (2005) and pig flue (2010) outbreaks in Turkey. In other words, while ontological insecurities lead to irrational reactions (morale panic) with the contribution of the media, serious measures , such as curfews of people over 65, are not taken too seriously despite all warnings of the Minister of Health and Science Committee. On the other hand, it can be said that the authoritarian tendencies towards broader measures such as the curfew proclamation in the whole country have increased.
Telemedicine for Eating Disorders: A Brief Report of a Italian Project
Authors: Savarese Giulia , Carpinelli Luna , Padovano Maria Rosaria , Pecoraro Nadia , Fasano Oreste , Mollo Monica , Giovanni Claudio Di , Politi Martino 
Published: May 2020

The objectives of the present work are the research and development of a Telemedicine system for therapy and enhanced monitoring of Eating disorders (ED). To this end, useful tools have been developed both for patients and caregivers and for operators: a) a mobile application for medical and psychological updates on the status of patients and through which it is possible to access psychological counselling services for family caregivers; and b) virtual and immersive reality viewers for the therapy of ED patients with social anxiety.
Interdisciplinarity, E-Mentoring and Support of Entrepreneurial Research Skills
Authors: Hamburg Ileana 
Published: May 2020

Innovation requires the development of entrepreneurial skills in order to provide a new approach to the work and solutions to a challenge in the corresponding enterprise. Research skills can help entrepreneurs throughout your career i.e. to develop an innovative project, to find an answer to a question. to find data and information about a topic, analyze and interpret this information to find a solution.
Mentoring is understood mostly as a supportive relationship to guide the successful integration of new members/entrepreneurs into an organization or to help entrepreneurs to be innovative and add value to the organization. E-mentoring helps a mentee to obtain knowledge with the support of a mentor who is physically not present but using digital technologies.
One important conceptual change regarding mentoring in both business and education is the emphasis on the need for more than a single mentor i.e. networks of mentors who may provide both technical careers advancing advice and psychosocial support. Interdisciplinary mentorship is a tool of scientists and entrepreneurs to help produce synergy in groups, and to generate multifocal ideas and complex solutions to complex challenges.
This paper examines entrepreneurial learning, mentoring and entrepreneurial research skills as main factors for development of innovation in companies. An example of a project in the context of achieving workplace-oriented research skills of entrepreneurs is given. A part of the project runs within the Coronavirus crisis, so entrepreneurs learn and interact mainly by using a digital platforms and E-mentors.
Posidonia oceanica lower depth limits along a latitudinal gradient in the eastern Adriatic Sea
Authors: Zubak Ivana , Cizmek Hrvoje , Mokos Melita 
Published: May 2020

AbstractAlthough extensive studies have been made on Posidonia oceanica ecosystems, in terms of their phenological characteristics, shoot density, biomass, and associated faunal assemblages, little attention has been given to the depth distribution of this species in the Adriatic Sea. The depth limits of the Mediterranean endemic seagrass P. oceanica growing along the eastern coast of the Croatian Adriatic Sea were examined by the use of SCUBA diving. Fifty-two independent measurements show that the lower depth limit of P. oceanica in the Croatian Adriatic Sea ranges from 24 m in the north to 36 m in the south, with a strong latitudinal gradient. The information on maximum depth distribution is an important asset when the increased human pressure and rapid environmental changes pose a threat to the survival of this slow-growing marine species.
Perfectionism and Loneliness: The Role of Expectations and Social Hopelessness in Daily Life
Authors: Harper Kelly L. , Eddington Kari M. , Silvia Paul J. 
Published: May 2020

Introduction: Research suggests that social disconnection is one pathway that explains why perfectionists have higher negative mental health outcomes. However, thus far research has not examined the role that perfectionists' expectations, about their social world, plays in explaining social disconnection. The current study examined whether negative expectations about future social events and social hopelessness explained the relations between socially prescribed perfectionism (SPP), self-oriented perfectionism (SOP), and social disconnection. Method: The current study used experience sampling methodology (ESM) with 145 participants to test multilevel structural equation models examining whether negative expectations and social hopelessness mediated the relation between SPP (as well as SOP) and loneliness at the following time point. Results: Results indicate that having negative expectations for future social interactions and feeling socially hopeless explains the relation between SPP and loneliness both when loneliness was measured concurrently and loneliness measured later in the day. Having lower levels of social hopelessness explained the relation between SOP and loneliness when loneliness was measured concurrently and later in the day. Discussion: Findings suggest that SPP and SOP are differently associated with cognitions about social interactions and relationships in daily life, which in turn explains variations in loneliness. Results indicate that targeting socially prescribed perfectionists’ negative social cognitions in treatment may be effective for reducing loneliness.
The Prevalence and Correlates of Suicidality in a Sample of Adolescents in Guangdong, China
Authors: Yan Pei , Ren Yaxuan , Li Minyi , Lin Min-Pei , Xu Sian , Liu Jiaqi , Zhou Guofu , Xu Liang , You Jianing 
Published: May 2020

Background: Suicidality is a public health concern among Chinese adolescents. This study aimed to examine the prevalence and correlates of suicidal ideation and suicide attempts in a large sample of Chinese adolescents.Methods: A total of 30,644 Chinese students (56.7% male; Mage = 14.14 years) completed the questionnaires, and a multinomial logistic regression was conducted to analyze the data.Results: About 17.0% of the participants reported having suicidal ideation and 4.4% reported suicide attempts in the past year. Adolescents with more rumination, more severe depressive symptoms, more communication problems with their parents, and more feelings of burdensomeness were more likely to be suicidal ideators or suicide attempters rather than non-suicidal controls. Additionally, male adolescents, adolescents from incomplete families, adolescents whose parents have lower educational levels, and adolescents with less rumination, more severe depressive symptoms, less communication problems with their parents were more likely to be suicide attempters rather than suicidal ideators.Discussion: Suicidality is prevalent and correlates with various familial and individual factors in Chinese adolescents. Theoretical and clinical implications of the findings were discussed.
Disruption oftmc1/2a/2bGenes in Zebrafish Reveals Subunit Requirements in Subtypes of Inner Ear Hair Cells
Authors: Smith Eliot T. , Pacentine Itallia , Shipman Anna , Hill Matthew , Nicolson Teresa 
Published: May 2020

Detection of sound and head movement requires mechanoelectrical transduction (MET) channels at tips of hair-cell stereocilia. In vertebrates, the transmembrane channel-like (TMC) proteins TMC1 and TMC2 fulfill critical roles in MET, and substantial evidence implicates these TMCs as subunits of the MET channel. To identify developmental and functional roles of this Tmc subfamily in the zebrafish inner ear, we tested the effects of truncating mutations intmc1,tmc2a, andtmc2bonin vivomechanosensation at the onset of hearing and balance, before gender differentiation. We find thattmc1/2a/2btriple-mutant larvae cannot detect sound or orient with respect to gravity. They lack acoustic-evoked behavioral responses, vestibular-induced eye movements, and hair-cell activity as assessed with FM dye labeling and microphonic potentials. Despite complete loss of hair-cell function,tmctriple-mutant larvae retain normal gross morphology of hair bundles and proper trafficking of known MET components Protocadherin 15a (Pcdh15a), Lipoma HMGIC fusion partner-like 5 (Lhfpl5), and Transmembrane inner ear protein (Tmie). Transgenic, hair cell-specific expression of Tmc2b-mEGFP rescues the behavioral and physiological deficits intmctriple mutants. Results fromtmcsingle and double mutants evince a principle role for Tmc2a and Tmc2b in hearing and balance, respectively, whereas Tmc1 has lower overall impact. Our experiments reveal that, in developing cristae, hair cells stratify into an upper, Tmc2a-dependent layer of teardrop-shaped cells and a lower, Tmc1/2b-dependent tier of gourd-shaped cells. Collectively, our genetic evidence indicates that auditory/vestibular end organs and subsets of hair cells therein rely on distinct combinations of Tmc1/2a/2b.SIGNIFICANCE STATEMENTWe assessed the effects oftmc1/2a/2btruncation mutations on mechanoelectrical transduction (MET) in the inner-ear hair cells of larval zebrafish.tmctriple mutants lacked behavioral responses to sound and head movements, while further assays demonstrated no observable mechanosensitivity in thetmc1/2a/2btriple mutant inner ear. Examination oftmcdouble mutants revealed major contributions from Tmc2a and Tmc2b to macular function; however, Tmc1 had less overall impact. FM labeling of lateral cristae intmcdouble mutants revealed the presence of two distinct cell types, an upper layer of teardrop-shaped cells that rely on Tmc2a, and a lower layer of gourd-shaped cells that rely on Tmc1/2b.
Evolution of Microbial Growth Traits Under Serial Dilution
Authors: Lin Jie , Manhart Michael , Amir Ariel 
Published: May 2020

AbstractSelection of mutants in a microbial population depends on multiple cellular traits. In serial-dilution evolution experiments, three key traits are the lag time when transitioning from starvation to growth, the exponential growth rate, and the yield (number of cells per unit resource). Here, we investigate how these traits evolve in laboratory evolution experiments using a minimal model of population dynamics, where the only interaction between cells is competition for a single limiting resource. We find that the fixation probability of a beneficial mutation depends on a linear combination of its growth rate and lag time relative to its immediate ancestor, even under clonal interference. The relative selective pressure on growth rate and lag time is set by the dilution factor; a larger dilution factor favors the adaptation of growth rate over the adaptation of lag time. The model shows that yield, however, is under no direct selection. We also show how the adaptation speeds of growth and lag depend on experimental parameters and the underlying supply of mutations. Finally, we investigate the evolution of covariation between these traits across populations, which reveals that the population growth rate and lag time can evolve a nonzero correlation even if mutations have uncorrelated effects on the two traits. Altogether these results provide useful guidance to future experiments on microbial evolution.