bluuebunny/crossref_metadata_2025_split huggingface dataset:
Features of immunophenotypic finding B-cell lymphoproliferative diseases by flow cytometry
Authors: Chuksina J J , Kataeva E V , Mitina T A 
Published: February 2020

Aim. To assess the information content of conventional and additional immunophenotypic markers (CD200, CD305) in the differential diagnosis B-cell lymphoproliferative diseases by flow cytometry.
Methods. An immunophenotypic study using 4-color flow cytometry was performed in 204 patients with different variants of B-cell non-Hodgkin's lymphomas. The study material included peripheral blood and bone marrow. The expression of CD45, CD19, CD20, CD22, CD79b, CD79a, CD5, CD10, CD23, FMC7, CD43, CD38, CD11c, CD103, CD25, CD 200, CD 305, light chains of immunoglobulins (kappa/lambda) using monoclonal antibodies (Becton Dickinson, USA) was evaluated. The intensity of antigen expression was assessed using mean fluorescence intensity (y. e.).
Results. Conventional FMC7-positive expression revealed only half patients with different variants of leukemization of non-Hodgkin's lymphomas, whereas atypical positive expression of CD23 was observed in patients with marginal spleen lymphoma and follicular lymphoma in 27.3 and 28.6% of cases, respectively. In mantle cell lymphoma, expression of CD200 in B-cell was detected in a significantly smaller number of observations, accompanied by a significant decrease in the average intensity of CD200 fluorescence compared to B-cell chronic lymphocytic leukemia (B-CLL) cells. The mean fluorescence intensity (MFI) of CD305 in hairy cell leukemia is significantly higher than in splenic marginal zone lymphoma (SMZL) with villous lymphocytes.
Conclusion. Different levels of the information content of some conventional markers were revealed in differential immunophenotypic diagnosis of B-cell lymphoproliferative diseases by flow cytometry; the use of additional markers CD200 and CD305 was highly informative in differential diagnostics between different variants of B-cell lymphoproliferative diseases with similar immunophenotypic and morphological characteristics of lymphoid elements.
PENINGKATAN MINAT DAN HASIL BELAJAR MATEMATIKA MATERI SEGIEMPAT DAN SEGITIGA MENGGUNAKAN MODEL PEMBELAJARAN (PAIKEM GEMBROT) PADA PESERTA DIDIK KELAS VII-D MTs NEGERI SALATIGA
Authors: Rahayuningtyas Esti , Istiqlal Muhammad 
Published: February 2020

This research aimed to increase the interest mathemathics learning and student achievement of class VII-D MTs N 1 Salatiga used PAIKEM Gembrot type.the types of research was Classroom Action Research (CAR). Research was conducted minimal two cycles action. Data wa collected by questionnair, observation, interview, test and documentation.result showed an increase student interest viewed from questionnair, observation, interview and test. Student achievement incresed to by 42,89 at pre-cycle to 85,53 in cycle I and incresed to 90,53 in cycle II. Therefore suggested to teachers use the PAIKEM Gembrot type to increase student interest and achievement.
ROLE OF THE ALKALINE FACTOR IN THE DEVELOPMENT OF DENTAL CARIES
Authors: Hamzaeb Bahlul , Jafarova Ayat , Huseynova Rena , Abbasova Rumia , Yusubova Shahla , Alizade aysel , Ibrahimova Lala 
Published: February 2020

Subject. This article analyzes existing theories about the prevalence and causes of dental caries, which is considered as a civilization disease.
The crucial role of endogenous factors in the risk of caries, including a pulp and dental fluid, is noted. Data are given that acids do not actively participate in the process of demineralization, which is confirmed by numerous theoretical and hypothetical points.
The role of some materials produced by dental industry based on this “pseudo-acidogenic” theory and used in the treatment of caries, as well as the false and negative consequences created by them in general, is analyzed, and adequate parallels are carried out.
The purpose of the study was the theoretical justification of the key role of acetylcholine (the cholinomimetic mediator located on the Toms fibers) in the occurrence of dental caries.
Methodology. Russian and foreign research works due to the etiology, prevalence and pathogenesis of dental caries were studied, and a comparative theoretical analysis of the available data on this issue was carried out.
Results. Based on the results of numerous studies cited in the available literature and our studies, we performed an analysis of theoretical principles and substantiated the data that the process of demineralization occurring in dental caries cannot be caused by acids. Therefore, the only and decisive factor in the origin of the demineralization process is the occurrence of the process because of alkaline substances.
Conclusions. Based on the studied references, we present the data that the most current theories of the etiopathogenesis of caries are imperfect in terms of solving the problem, which, in our opinion, requires further study of the process both of acetylcholine and the enzyme acetylcholinesterase inhibition in the local form and inside dental hard tissues.
Las parteras, patrimonio de la humanidad
Authors: Alarcón-Nivia Miguel Ángel , Sepúlveda-Agudelo Janer , Alarcón-Amaya Iván Camilo 
Published: February 2020

Objetivo: presentar el origen etimológico del término partera. Describir las parteras que han hecho aportes a la obstetricia a través de la historia. Hacer una reflexión . sobre el desempeño actual de las parteras en la saludMateriales y métodos: se presentan las parteras más famosas o que hicieron aportes a la tocología o la salud pública a través de los siglos. Se describe el papel de las parteras hoy día en las diferentes regiones del mundo.Conclusión: las parteras continúan jugando un importante papel en la salud materna en varios países del mundo, dado que tienen amplia aceptación en las comunidades, reciben capacitación en buenas prácticas y pueden detectar las alteraciones tempranas del embarazo y el parto; sin embargo, en otros países este oficio se halla prácticamente en el ocaso, está reducido a zonas marginales y no cuentan con apoyo de los organismos de salud
Generał Wacław Iwaszkiewicz-Rudoszański (1871–1922) – zarys biografii
Authors: Jarno Witold 
Published: February 2020

Artykuł opisuje życie generała Wacława Iwaszkiewicza-Rudoszańskiego (1871–1922). Urodził się on w Omsku, w polskiej rodzinie zesłanej na Syberię po powstaniu styczniowym. Był zawodowym oficerem armii rosyjskiej, w której służył w różnych jednostkach na Dalekim Wschodzie. Brał udział w tłumieniu tzw. powstania bokserów w Chinach oraz w wojnie rosyjsko-japońskiej 1904–1905. Po wybuchu pierwszej wojny światowej dowodził 54 pułkiem strzelców syberyjskich. W 1915 r. został awansowany na stopień generała. W armii rosyjskiej służył do 1917 r., a następnie dowodził 3 Dywizją Strzelców w I Korpusie Polskim w Rosji. Po odzyskaniu przez Polskę niepodległości zgłosił się do Wojska Polskiego i kolejno dowodził: Okręgiem Generalnym nr III Kielce, Dywizją Litewsko-Białoruską, a od marca 1919 r. był dowódcą Wojsk Polskich na Galicję Wschodnią. Dowodził wówczas odsieczą dla Lwowa, która uwolniła miasto od zagrożenia ze strony wojsk ukraińskich. W latach 1919–1920 dowodził kolejno: Frontem Galicyjskim, 6 Armią oraz Frontem Południowym. Następnie – od września 1920 r. do maja 1921 r. – był dowódcą Okręgu Generalnego numer I Warszawa. Wskutek choroby został przeniesionyw stan spoczynku i niebawem zmarł w Warszawie (pochowany został we Lwowie). Jako jeden z nielicznych był odznaczony Orderem Virtuti Militari II klasy.
Pengaruh pengencer dan lama simpan semen Ayam kampung pada suhu ruang terhadap Motilitas dan viabilitas spermatozoa
Authors: Ulus Egidius , Kusumawati Enike Dwi , Krisnaningsih Aju Tjatur Nugroho 
Published: February 2020

Abstract
The study was conducted from February to April 2018 in the field laboratory and integrated laboratory of University Kanjuruhan Malang. The purpose of this research is to know the effect of diluent and timetorage of kampung chicken sperm at room temperature to motility and viability of spermatozoa. The diluent used was NaCl, egg yolk tris aminomethane and no diluent with sperm duration of 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30 hours and 10 replications. This research method is experimental laboratory by using completely randomize design of factorial. The data obtained in the study will be analyzed using the method of analysis of variance. The results showed that there was no significant effect (P> 0,05) of diluent, time storage as well as interaction between diluent and time storage to sperm motility and viability. Diluent and time storage which still maintain motility up to the minimum limit for AI was NaCl in 18 hours (41,5%), egg yolk tris aminomethan and without diluent at 12 hours of time storage that was equal to 49% and 42%. While the diluent and the time store that still can maintain viability up to the minimum limit for AI was NaCl and egg yolk tris aminomethan on the 18-hour (82.5%), without diluent on the time storage 12 hours (81.5%). It can be concluded that the optimum spermatozoa motility on NaCl diluent with 6 hours of time storage. While the optimum viability of the NaCl and egg yolk tris aminomethan on the time storage 18 hours.
Aspiration-Sclerotherapy Versus Laparoscopic De-roofing in the Treatment of Renal Cysts: Which is better?
Authors: Zhang Xueling , Cao Dehong , Han Peizhen , Ren Zhengju , Wang Jia , Wei Qiang 
Published: February 2020

Abstract
Purpose: To compare the clinical efficiency between aspiration-sclerotherapy (AS) and laparoscopic de-roofing (LD) in the management of renal cysts through meta-analysis of comparative studies. Method: A comprehensive literature search was performed by PubMed, MEDLINE, Ovid and Web of Science for relevant studies published up to January 2020. The statistical analyses were conducted with Review Manager 5.3.0 and Stata 15.1. The sensitivity analysis was also carried out to confirm the reliability of this Meta-analysis. Results: Our searches of literature generated 6 studies (1547 patients incorporated) comparing AS with LD in the impacts of renal cyst therapy. Of these, 6 studies contained 1106 and 441 patients who were treated with AS and LD, respectively. The outcome of this meta-analysis indicated that LD group was superior in symptomatic successful rate [Odds Ratio(OR): 0.28; 95%Confidence Interval(CI): 0.09 to 0.86；P=0.03), radiological successful rate (OR: 0.06; 95%CI: 0.02 to 0.15; P<0.01) and recurrence rate (OR: 6.08; 95%CI: 2.81 to 13.15; p<0.01). Nevertheless, AS group had shorter treatment time [Mean Difference(MD)：-51.10; 95% CI：-73.01 to -29.20; p<0.01]. No statistically significant difference was showed in the rate of complications (OR: 3.19; 95% CI: 0.39 to 25.88; P=0.28). Conclusion: In our meta-analysis, LD had higher symptomatic successful rate, radiological successful rate as well as lower recurrence rate than AS, while the treatment time was longer.
Self-Analysis of Repeat Proteins Reveals Evolutionarily Conserved Patterns
Authors: Merski Matthew , Młynarczyk Krzysztof , Ludwiczak Jan , Skrzeczkowski Jakub , Dunin-Horkawicz Stanisław , Górna Maria W. 
Published: February 2020

Abstract
Background Protein repeats can confound sequence analyses because the repetitiveness of their amino acid sequences lead to difficulties in identifying whether similar repeats are due to convergent or divergent evolution. We noted that the patterns derived from traditional “dot plot” protein sequence self-similarity analysis tended to be conserved in sets of related repeat proteins and this conservation could be quantitated using a Jaccard metric. Results Comparison of these dot plots obviated the issues due to sequence similarity for analysis of repeat proteins. A high Jaccard similarity score was suggestive of a conserved relationship between closely related repeat proteins. The dot plot patterns decay quickly in the absence of selective pressure with an expected loss of 50% of Jaccard similarity due to a loss of 8.2 % sequence identity. We assembled a standard set of 79 repeat proteins representing all the subgroups in RepeatsDB to perform method testing on. Comparison of known repeat and non-repeat proteins from the PDB suggested that the information content in dot plots could be used to identify repeat proteins from pure sequence without needing structural information. Analysis of the UniRef90 database suggested that 16.9% of all known proteins could be classified as repeat proteins. These 13.3 million putative repeat protein chains were clustered and a significant amount (82.9%) of clusters containing between 5 and 200 members were of a single functional type. Conclusions Dot plot analysis of repeat proteins attempts to obviate issues that arise due to the sequence degeneracy of repeat proteins. These results show that this kind of analysis can efficiently be applied to analyze repeat proteins on a large scale.
Storage time and temperature affect the isolation rate of Mannheimia haemolytica and Pasteurella multocida from bovine bronchoalveolar lavage samples
Authors: Driessche Laura Van , De Neve Charlotte , Haesebrouck Freddy , Leenen Katharina van , Boyen Filip , Pardon Bart 
Published: February 2020

Abstract
Background: A microbiological diagnosis is essential to better target antimicrobial treatment, control and prevention of respiratory tract infections in cattle. Under field conditions, non-endoscopic broncho-alveolar lavage (nBAL) samples are increasingly collected. To what extent the highly variable turnaround time and storage temperatures between sampling and cultivation affect the isolation rate of bacterial pathogens is unknown. Therefore, the objective of this experimental study was to determine the effect of different storage temperatures (0°C, 8°C, 23°C and 36°C) and times (0,2,4,6,8,24,48 hours) on the isolation rate and concentration of Pasteurellaceae in nBAL samples from clinically affected animals. Results: At a storage temperature temperature of 36°C isolation rates of Mannheimia haemolytica and Pasteurella multocida were significantly reduced 6h and 48h after sampling, respectively. At room temperature (23°C), a decrease in M. haemolytica and P. multocida isolation rate was noticed, starting at 24 and 48 hours after sampling, respectively, but only significant for P. multocida at 48h. The presence of microbial contamination negatively affected the isolation of P. multocida in clinical nBAL samples, but not of M. haemolytica. Conclusion: Optimal M. haemolytica and P. multocida isolation rates from clinical nBAL samples are obtained after storage at 0°C or 8°C, provided that the sample is cultivated within 24 hours after sampling. The maximum period a sample can be stored without an effect on the M. haemolytica and P. multocida isolation success varies and is dependent on the storage temperature and the degree of microbial contamination.
Neuropathological changes in dorsal root ganglia induced by pyridoxine in dogs
Authors: Yun Sumin , Kim Woosuk , Kang Min Soo , Kim Tae Hyeong , Kim Yoonhwan , Ahn Jin-Ok , Choi Jung Hoon , Hwang In Koo , Chung Jinyoung 
Published: February 2020

Abstract
Background: Pyridoxine (PDX), vitamin B6, is an essential vitamin. PDX deficiency induces various symptoms and abuse of PDX supplement also acts as a neurotoxicant that induces severe sensory neuropathy. Results: To assess the possibility of reversible sensory neuropathy model using dogs, 150 mg/kg pyridoxine (PDX) was injected subcutaneously into dogs for seven days and body weight measurement, postural reaction assessments, and electrophysiological recordings were conducted. In addition, the morphology of dorsal root ganglia (DRG) and changes of glial fibrillary acidic protein (GFAP) immunoreactive satellite glial cells and ionized calcium-binding adapter molecule 1 (Iba-1) immunoreactive microglia/macrophages were observed at 0, 1 and 4 weeks after the last PDX treatment. During the administration period, body weight loss and proprioceptive loss occurred. After the treatments were completed, electrophysiological recordings showed that the H-reflex of the treated dogs had disappeared at week 0. These phenomena persisted for four weeks exceptional body weight. CV and HE staining revealed that neurons in DRG had significant loss of large-sized neurons 0 and 1 week, but these neurons were recovered 4 weeks. Iba-1 and GFAP immunohistochemistry showed that reactive microglia/macrophages and satellite glial cells were pronounced at 0 and 1 weeks after the last PDX treatment, respectively and thereafter decreased with time after PDX treatment, respectively.Conclusions: This result suggests that PDX-induced neuropathy model is reversible and can be a good experimental model for research on neuropathy in dogs.
The Geriatric Nutrition Risk Index versus the Mini-Nutritional Assessment Short Form in predicting postoperative delirium and hospital length of stay among older non-cardiac surgical patients: a prospective cohort study
Authors: Zhao Yanli , Ge Ning , Xie Dongmei , Gao Langli , Wang Yanyan , Liao Yulin , Yue Jirong 
Published: February 2020

Abstract
Backgrounds : Malnutrition has been shown to be associated with poor prognosis in older surgical patients. Several tools are available for detecting malnutrition. But little is known about their ability to assess risks of postoperative adverse outcomes. The study aimed to compare the ability of the Geriatric Nutritional Risk Index (GNRI) and the Mini-Nutritional Assessment Short Form (MNA-SF) in predicting postoperative delirium (POD) and length of stay (LOS) among older non-cardiac surgical patients.
Methods : Prospective study of 288 older non-cardiac surgical patients from the West China Hospital of Sichuan University. Preoperative nutritional status was assessed using the GNRI and MNA-SF, and patients were followed for the occurrence of POD and LOS. Multivariable logistic regression and linear regression analyses were used to identify predictors of these outcomes. The relative performance of the GNRI and MNA-SF as predictors of these outcomes were determined by Receiver Operating Characteristic curves (ROC) analyses and the area under the curve (AUC) .
Results : Multivariable analysis revealed that preoperative malnutrition by the MNA-SF was significantly associated with POD. Linear regression analysis showed that preoperative low/high nutritional risk of the GNRI and malnutrition by the MNA-SF were independent predictors of prolonged LOS. Moreover, the area under the curve (AUC) of MNA-SF scores for POD was better than GNRI scores (AUC=0.718, 95%CI: 0.64-0.80, P <0.001 vs AUC=0.606, 95%CI: 0.52-0.69, P= 0.019; Delong’s test, P=0.006), but the AUC of GNRI scores and MNA-SF scores have no significant difference when predicting prolonged LOS (AUC=0.611, 95%CI: 0.54-0.69, P= 0.006 vs AUC=0.533, 95%CI: 0.45-0.62, P= 0.421; Delong’s test, P=0.079).
Conclusion : The MNA-SF was more effective than the GNRI at predicting the development of POD, but the two nutrition screening methods have similar performance in predicting prolonged LOS among older non-cardiac surgical patients.
Relationships of Accelerometer-based Measured Objective Physical Activity and Sedentary Behaviour with Cognitive Function: A Comparative Cross-sectional Study of China’s Elderly Population
Authors: Wu Zhi jian , Wang Zhu ying , Hu Bing qian , Zhang Xu hui , Zhang Fan , Wang Hou lei , Li Fang hui 
Published: February 2020

Abstract
This study explored the effects of physical activity and sedentary behaviour on the decline of cognitive ability among the elderly. To compensate for the limitations of self-reported physical activity, objective measures were used. A cross-sectional survey of 308 aged people mean 68.66±5.377 years, in Nanjing, China, was conducted. Physical activity was measured using the ActiGraph GT3X+, and cognitive function was measured using the Montreal Cognitive Assessment. The overall participant model, adjusted for age, BMI, education, and monthly average income, found that light physical activity (β=0.006, p<0.01), moderate-vigorous physical activity (β=0.068, p<0.001), and total physical activity (β=0.006, p<0.01) had a significant linear relationship with cognitive ability, while sedentary time did not (β=-0.020, p＞0.05). Further, light physical activity only affects the cognitive ability of elderly females (β=0.006, p<0.05). There was an inverted ‘U’ association between moderate-vigorous physical activity and cognitive ability. The association models found that moderate-vigorous physical activity in the 22.13 min·day –1 ～38.79 min·day –1 range affected cognitive ability most beneficially, with the highest beta coefficient among all groups (β=0.091, p<0.05). In conclusion, while physical activity can significantly improve cognitive ability among the elderly, sedentary behaviour is associated with decreased cognitive function across genders.
Prognostic significance of SDF-1 in colorectal cancer depends on CD8+ T-cell density
Authors: Lalos Alexandros , Tülek Ali , Tosti Nadia , Mechera Robert , Wilhelm Alexander , Soysal Savas , Daester Silvio , Kancherla Venkatesh , Weixler Benjamin , Spagnoli Giulio C. , Eppenberger-Castori Serenella , Terracciano Luigi , Piscuoglio Salvatore , Flüe Markus von , Posabella Alberto , Droeser Raoul 
Published: February 2020

Abstract
Background: Since colorectal cancer (CRC) remains one of the most common malignancies, a tremendous amount of studies keep taking place in this field. Over the past 25 years, a notable part of the scientific community has focused on the association between the immune system and colorectal cancer. A variety of studies have shown that high densities of infiltrating CD8+ T cells are associated with improved disease-free and overall survival in colorectal cancer (CRC). Stromal cell-derived factor-1 (SDF-1) is a protein that regulates leukocyte trafficking and is variably expressed in several healthy and malignant tissues. There is strong evidence that SDF-1 has a negative prognostic impact on colorectal cancer (CRC). However, based on a significant correlation of SDF-1 and CD8+ T cells in a previous study (r=0.53, p<0.0001), we hypothesized that the prognostic significance of SDF-1 in CRC could depend on the immune microenvironment. Therefore, we explored the combined prognostic significance of SDF-1 expression and CD8+ T cell density in a large CRC collective. Methods: We analyzed a tissue microarray (TMA) of 613 patient specimens of primary CRCs by immunohistochemistry (IHC) for the expression of SDF-1 by tumor cells and tumor-infiltrating immune cells (TICs) and CD8+ T-cells. Besides, we analyzed the expression of SDF-1 at the RNA level in The Cancer Genome Atlas cohort (TCGA). Results: We found that the the combined high expression of SDF-1 and CD8+ T-cell infiltration shows a favorable 5-year overall survival rate (66%; 95%CI=48–79%) compared to tumors showing a high expression of CD8+ T-cells only (55%; 95%CI=45–64%; p=0.0004). High expression of SDF-1 and CD8+ T-cells infiltration was significantly associated with a favorable prognosis also in a validation group (p=0.016). Univariate and multivariate Hazard Cox regression survival analysis considering the combination of both markers revealed that the combined high expression of SDF-1 and CD8+ T cells was an independent, favorable, prognostic marker for overall survival (HR=0.34, 95%CI=0.17–0.66; p=0.002 and HR=0.45, 95%CI=0.23–0.89; p=0.021, respectively). In a spearman’s correlation analysis from the TCGA cohort, SDF-1 also correlated significantly with CD8+ T cells (r=0.28). Conclusions: SDF-1 high /CD8 high density represents an independent, favorable, prognostic condition in CRC, most likely due to an effective antigen-specific immune response.