bluuebunny/crossref_metadata_2025_split huggingface dataset:
OFAT versus DOE as two optimization protocols for the chromatographic analysis of some OTC pharmaceuticals carrying negative cardiovascular effects and administered by pregnant and breast-feeding females: Application to dose dependent effect
Authors: El Sherbiny Dina , Wahba Mary E. K. 
Published: April 2020

AbstractA simple HPLC technique has been utilized for rapid and sensitive quantitative analysis of two mixtures of drugs that are used during pregnancy and lactation. Drugs of the first mixture are used to manage gastrointestinal tract illness that are common during early stages of pregnancy, while pharmaceutical agents of the second mixture are administered over the counter as galactagogues or to overcome postpartum depression. Mixture I includes famotidine (FMT), ranitidine (RNT), nizatidine (NZT), and pantoprazole (PNT), which were separated on a C18 column using a mobile phase composed of methanol: 0.02 M sodium dihydrogen phosphate (60:40, v/v) of pH 6.9, adopting UV detection at 240 nm at a flow rate of 1 mL/min. Mixture II on the other hand, consists of domperidone (DOM), metoclopramide (MET), and sulpiride (SUL). These drugs were eluted using the same column and flow rate as those in mixture I, using a mobile phase consisting of acetonitrile: 0.075 M sodium dihydrogen phosphate (30:70, v/v) of pH 6 adopting a detection wavelength 270 nm. Two optimization protocols were utilized to optimize the chromatographic separation conditions, namely one factor at a time (OFAT) and design of experiments (DOE) where face centered cube response surface experimental design was chosen for this investigation. Comparison of the results obtained from both protocols reveals the accordance between them.Full validation procedure under guidance of United States Pharmacopoeia (USP) was applied to the proposed methods which enabled their application to separate the drugs of both mixtures in spiked rat whole blood samples and in vivo analysis of rat heart blood.
Evaluation of Agro-Industrial by Products as Potential Local Feed for Ruminant Animals: Chemical Composition, Fiber Fractions and In Vitro Rumen Fermentation
Authors: Pratama Said Mirza , Wajizah Sitti , Jayanegara Anuraga , Samadi Samadi 
Published: April 2020

Animals are considered as source of protein should be improved their productivity with the minimum cost production. Agro-industrial by products have been used as animal feed to reduce feed cost. The purpose of this study is to evaluate agro-industrial by product in Aceh as potential local feed for ruminant animals based on chemical composition, fiber fraction and in vitro rumen fermentation. There were eight sources of agro-industrial by products (sago residues, coconut meal, soybean-ketchup by product, coffee pulp, cacao pod, sago tree, corncob, and rice brand) which were collected from 3 different locations in Aceh. All agro-industrial by product samples were dried at 600C for 24 h and ground to pass a 1 mm sieve. Grounded samples were analyzed to determine chemical composition, fiber fractions and in vitro rumen fermentation. Incubation was conducted at temperature 390C for 48 h in water bath with three replicates. Data for in vitro rumen fermentation were statically calculated by using SPSS differences between treatments were stated (P≤0.05) by using Duncan Multiple Range Test (DMRT). The results indicated that agro industrial by product from coconut meal, ketchup residues, coffee by product, cacao by product, and rice brand can be used as source of protein and industrial by product from sago by product, sago tree and corncob can be used as source of energy. Neutral detergent insoluble CP (NDICP) and Acid detergent insoluble CP (ADICP) were relatively high for sago by product, sago tree and rice brand but relatively low for cacao by product and corncob. The value of incubated pH for most feed samples was in the normal range. In vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD) were significantly difference (P≤0.05) each agro-industrial by products with the highest for sago and the lowest for coffee by product. In conclusion, agro-industrial by products had a potential feed for ruminant animals both as protein and energy sources. However, feed ingredients with high fiber content and low degradability, further treatments such as physical, chemical and biological treatments were required to improve the feed quality.
Calves Productivity with Applying Integrated Village Management System (IVMS) and Rice-Straw Based Feed
Authors: Ratnawati Dian , Dikman Dicky Mohammad , Affandhy Lukman 
Published: April 2020

The objective of the study was to assess the productivity of PO calves with Integrated Village Management System (IVMS). The calf management generally follows cow pattern. The IVMS management applied to calves include weaning 5-6 months and optimizing local feed. Straw-based cow feed with supplementation using greenfeed and concentrate. Recording productivity of calf include: birth, weaning, body weight, calf death calf mobilization. Weighing weight was done at birth, weaning, and every month in the first year of study and every 2 months in the next research year. Implementation of IVMS management with rice straw-based feed produces good PO calf productivity when supported by addition of other feed sources, i. e. feed with high protein composition (concentrate, legume) to balancing the nutrient composition of feed.
Development of a Control Strategy for a Smart Sucker Rod Pump
Authors: Oliva G. B. , Galvão H. L. , Silva R. E. , Costa R. O. , Carratore P. R. , Maitelli A. L. , Maitelli C. W. 
Published: April 2020

Summary
The sucker-rod pumping (SRP) system is the most used artificial lift method in oil wells in Brazil and in the world. This method stands out for its simplicity and low cost in investments and maintenance, and can be operated in a large range of flow rates, with fluids of different compositions and viscosities. However, the SRP units as well as all other mechanical equipment require periodic adjustments and maintenance, whether corrective or preventive. Two important procedures commonly made at the SRP units are the adjustment for counterbalancing the pumping unit and the adjustment of the polished rod stroke length. Performing these procedures requires stopping the production of the well. Usually, such adjustment procedures are made manually by a work team that is transported to the place where the pumping unit is located. In this context, Petrobras in partnership with Federal University of Rio Grande do Norte and other companies developed a new type of pumping unit, called smart sucker rod pump (SSRP), which is capable of counterbalancing the unit and adjusting the polished rod stroke length without mobilizing a team to go to the field. Thus, this work proposes to develop a strategy to automatically control the SSRP. The development of this strategy aims to ensure that the equipment operates under proper conditions and within its safety limits (previously set by the operator), as well as to optimize the well production. In order to test the control strategy developed for the SSRP, simulations were performed using synthetic wells. It was observed that the developing strategy reached the proposed goals for which it was developed; that is, to keep the parameters of the SSRP within the limits set by the operator and optimize the production flow rate.
Challenges and barriers of Humanitarian Aid Management in 2017 Kermanshah Earthquake: A Qualitative Study
Authors: Safarpour Hamid , Fooladlou Saeideh , Safi-Keykaleh Meysam , Mousavipour Somayyeh , Pirani Davoud , Sahebi Ali , Ghodsi Hassan , Farahi-Ashtiani Iman , Dehghani Arezoo 
Published: April 2020

Abstract
Background: Examining various problems after disasters is important for the affected people. Managing humanitarian aid and donations among the affected people is considered as one of the most important problems after disasters. Therefore, the present study aimed to evaluate the challenges and barriers of humanitarian aid management in 2017 Kermanshah Earthquake. Methods: This study was conducted from November 2017 to January 2018, with qualitative case study design. The population included 21 people including 6 humanitarian aid manager, 6 volunteers, 4 aid workers, and 5 affected people. The data were collected through semi-structured interviews and purposeful sampling, which continued until saturating the data. The strategies recommended by Guba were used for evaluating the trustworthiness of the data. The data was analyzed with conventional content analysis method according to method suggested by Graneheim and Lundman. Results: Based on the results, two themes, nine categories, and 19 sub-categories were identified considering the challenges and barriers of humanitarian aid and donors’ management during the 2017 Kermanshah Earthquake. The categories included education, command and coordination, communication and information, rules, security, traffic and overcrowding, assessment, providing system, and cultural setting. Also, two themes including managerial and structural barriers were extracted. Conclusion: Adopting an effective management and appropriate policies with respect to humanitarian aid and modifying structural and managerial barriers can improve the performance and management of humanitarian aid.
Linkage Mapping and Genome-Wide Association Study Reveals Conservative QTLs and Candidate Genes for Fusarium Rot Resistance in Maize
Authors: Wu Yabin , Zhou Zijian , Dong Chaopei , Chen Jiafa , Ding Junqiang , Zhang Xuecai , Mu Cong , Chen Yuna , Li Xiaopeng , Li Huimin , Han Yanan , Wang Ruixia , Sun Xiaodong , Li Jingjing , Dai Xiaodong , Song Weibin , Chen Wei , Wu Jianyu 
Published: April 2020

AbstractBackground: Fusarium ear rot (FER) caused by Fusarium verticillioides is a major disease of maize that reduces grain yield and quality globally. However, there have been few reports of major loci for FER were verified and cloned. Result: To gain a comprehensive understanding of the genetic basis of natural variation in FER resistance, a recombinant inbred lines (RIL) population and one panel of inbred lines were used to map quantitative trait loci (QTL) for resistance. As a result, a total of 10 QTL were identified by linkage mapping under four environments, which were located on six chromosomes and explained 1.0%-7.1% of the phenotypic variation. Epistatic mapping detected four pairs of QTL that showed significant epistasis effects, explaining 2.1%-3.0% of the phenotypic variation. Additionally, 18 single nucleotide polymorphisms (SNPs) were identified across the whole genome by genome-wide association study (GWAS) under five environments. Compared linkage and association mapping revealed five common intervals located on chromosomes 3, 4, and 5 associated with FER resistance, four of which were verified in different near-isogenic lines (NILs) populations. GWAS identified three candidate genes in these consistent intervals, which belonged to the Glutaredoxin protein family , actin-depolymerizing factors (ADFs), and AMP-binding proteins. In addition, two verified FER QTL regions were found consistent with Fusarium cob rot (FCR) and Fusarium seed rot (FSR). Conclusions: These results revealed that multi pathways were involved in FER resistance, which was a complex trait that was controlled by multiple genes with minor effects, and provided important QTL and genes, which could be used in molecular breeding for resistance.
Modified Staff-ball Technique for Densely Vegetated Areas
Authors: Pop Paul 
Published: April 2020

Abstract

Staff-ball technique is a method used to measure the horizontal cover of vegetation in an area. For the occupancy study of two threatened avian species, a modified version of this method has been developed, employed and standardized in the field, to collect data that has been used as proxy for visibility of these species through the undergrowth in plantations within Western Ghats, India. This modified cost-effective technique is very useful in areas which are highly vegetated with undulating terrain, as opposed to its original use in grasslands. Two staffs and a ball are the main equipment used, wherein a central staff with ball is placed within vegetation and viewed using a peripheral staff at a fixed height from different points in an imaginary circle. A weakness of this technique is the difficulty in alignment with the central staff-ball, which may be rectified by the use of a laser rangefinder.
Whole Genome Sequencing of Nontuberculous Mycobacterium (NTM) Isolates from Sputum Specimens of Co-Habiting Patients with NTM Pulmonary Disease and NTM Isolates from Their Environment
Authors: Yoon Jung-Ki , Kim Taek Soo , Kim Jong-Il , Yim Jae-Joon 
Published: April 2020

Abstract
Background: Nontuberculous mycobacterium (NTM) species are ubiquitous microorganisms. NTM pulmonary disease (NTM-PD) is thought to be caused not by human-to-human transmission but by independent environmental acquisition. However, recent studies using next-generation sequencing (NGS) have reported trans-continental spread of Mycobacterium abscessus among patients with cystic fibrosis. Results: We investigated NTM genomes through NGS to examine transmission patterns in three pairs of co-habiting patients with NTM-PD who were suspected of patient-to-patient transmission. Three pairs of patients with NTM-PD co-habiting for at least 15 years were enrolled: a mother and a daughter with M. avium-PD, a couple with M. intracellulare-PD, and a second couple, one of whom was infected with M. intracellulare and the other of whom was infected with M. abscessus. Whole genome sequencing was performed using patients’ NTM isolates as well as environmental specimens. Genetic distances were estimated based on single nucleotide polymorphisms (SNPs). By comparison with the genetic distances among 78 publicly available NTM genomes, NTM isolates derived from the two pairs of patients infected with the same NTM species were not closely related to each other. In phylogenetic analysis, the NTM isolates from patients with M. avium-PD clustered with isolates from different environmental sources.Conclusions: In conclusion, considering the genetic distances between NTM strains, the likelihood of patient-to-patient transmission in pairs of co-habiting NTM-PD patients without overt immune deficiency is minimal.
Morin inhibits Listeria monocytogenes virulence in vivo and in vitro by targeting listeriolysin O and inflammation
Authors: Li Gen , Wang Guizhen , Li Meng , Li Li , Liu Hongtao , Sun Meiyang , Wen Zhongmei 
Published: April 2020

Abstract
Background: Listeria monocytogenes ( L. monocytogenes ) is a global opportunistic intracellular pathogen that can cause many diseases, including meningitis and abortion in humans and animals; thus, L. monocytogenes poses a great threat to public safety and the development of the aquaculture industry. The pore-forming toxin listeriolysin O (LLO) is one of the most important virulence factors of L. monocytogenes ; LLO can promote cytosolic bacterial proliferation and aid in evading the attacks of the host immune system. In addition, L. monocytogenes infection can trigger a series of severe inflammatory reactions. Results: Here, on the basis of our previous studies, we further confirmed that morin lacking anti-Listeria activity could inhibit LLO oligomerization and found that morin could effectively alleviate the inflammation induced by Listeria in vivo and in vitro and has an obvious protective effect on infected cells and mice. Conclusions: Thus, we further prove that morin is a valuable drug precursor to be developed for the treatment of Listeria infection.
Evidence of an Increased Prevalence of Multiple Sclerosis: A Population-Based Study of Tehran Registry during 1999–2018
Authors: Almasi-Hashiani Amir , Sahraian Mohammad Ali , Eskandarieh Sharareh 
Published: April 2020

Abstract
Background: The epidemiological characteristics of multiple sclerosis (MS) have been investigated in various studies, which have revealed that the prevalence of MS varies across countries. The present study was conducted to investigate the longitudinal prevalence of MS in Tehran, Iran. Methods: The present population-based study was conducted in Tehran, the capital of Iran from 1999 to 2018 based on the annual report data provided by the Iranian MS Society (IMSS) registry system. The age-standardized and crude prevalence were estimated using population data presented by the Statistical Centre of Iran. Results: A total of 21,580 MS cases were registered and included in the analysis. Among the participant patients, 24.99% (5,393) and 75.01% (16,187) of cases were male and female, respectively. The mean age of MS onset was 28.8 years (S.D: 8.7). The age-standardized prevalence (ASP) of MS increased from 73.7 (95%CI: 72.1-75.2) per 100,000 people in 2006 to 137.6 (95% CI: 135.7-139.5) per 100,000 people in 2018. The ASP of MS in 2018 was estimated to be 67.9 (95%CI: 66.0-69.8) and 207.3 (95%CI: 204.0-210.7) per 100,000 people among males and females, respectively. The age-standardized female-to-male ratio of MS ranged from 3.7 (in 2010) to 2.06 (in 2017). Conclusion: The findings of this study suggested that the prevalence of MS in Tehran province is relatively high, and the occurrence of the disease is more common in the age groups under 40 years as compared with older-aged groups. In line with reports provided for various regions of the world, the prevalence of MS was higher among women. Similarly, the findings of this study revealed that the female-to-male ratio was 2.14 in 2018.
Immediate Effects of Urgent Reorganisation of Emergency Department-Based Treatment Pathway in Nonperforated Appendicitis: A Retrospective Study
Authors: Ojakäär Artur , Purdy Martin , Kechagias Aristotelis , Järvelin Ulla , Palomäki Ari 
Published: April 2020

Abstract
Background Acute appendicitis is a global disease and a very common indication for emergency surgery worldwide. The need for hospital resources is therefore constantly high. The administration in Kanta-Häme Central Hospital, Southern Finland, called for an urgent reorganisation due to shortage of hospital beds at the department of general surgery. According to it, postoperative treatment pathway of patients with nonperforated acute appendicitis was ordered to take place in the Emergency Department (ED). The aim of this study was to assess whether this reorganisation affected the length of in-hospital stay (LOS) and the 30-day complication rate.Methods This is a retrospective pre- and post-intervention analysis. After the reorganisation, most patients with nonperforated appendicitis were followed postoperatively at the 24-hour observation unit of the ED instead of surgical ward. Patients operated during the first three months after the reorganisation were compared to those operated during the three months before it. A case met inclusion criteria if there were no signs of appendiceal perforation during surgery. Exclusion criteria comprised age <18 years and perforated disease. Results During the study period, appendicectomy was performed on 112 patients, of whom 62 were adults with nonperforated appendicitis. Twenty-seven of the included patients were treated before the reorganisation, and 35 after it. Twenty of the latter were followed only at the ED. Postoperative LOS decreased significantly after the reorganisation. Median postoperative time till discharge was 15.7 hours for all patients after the reorganisation compared to 24.4 hours before the reorganisation (standard error 6.2 hours, 95% confidence interval 2.3–15.2 hours, p < 0.01). There were no more complications in the group treated postoperatively in the ED. Conclusions Early discharge of patients with nonperforated appendicitis after enforced urgent reorganisation of the treatment pathway in the ED observation unit is safe and feasible. Shifting the postoperative monitoring and the discharge policy of such patients to the ED – instead of the surgical ward – occurred in the majority of the cases after the reorganisation. This change may spare resources as in our series it resulted in a significantly shorter LOS without any increase in the 30-day complication rate.
Feasibility investigation of uniportal video-assisted thoracoscopic anatomical lung resection for pulmonary sequestration
Authors: Sun Yungang , Shao Feng , Zhang Qiang , Wang Zhao 
Published: April 2020

Abstract
Background Uniportal video-assisted thoracic surgery (UVATS) technique has been increasingly used for many thoracic diseases. Whether UVATS has equivalent or better perioperative outcomes for pulmonary sequestration (PS) patients remains controversial. Our study aimed to evaluate the feasibility of UVATS in anatomical lung resection for pulmonary sequestration. Methods A total of 24 patients with PS including fifteen males and nine females with the mean age of 40 (rang, 18-65) years old, who had received completely UVATS anatomical lung resection for PS in Nanjing Chest Hospital between January 2016 and December 2018 were retrospectively reviewed. Related clinical data were retrieved from hospital records and analyzed. Results All 24 patients had been treated with the UAVTS approach successfully without aberrant artery ruptured or massive hemorrhage, and no patients died during the perioperative period. Overall median surgery time was 102 mins (range, 55-150mins), the mean blood loss was 94 ml (range, 10-300ml), the mean days of chest tube maintained were 4 days (range,1-10days), and the mean postoperative hospitalization days was 6 days (range,2-11days). All patients were cured, without cough, fever, hemoptysis, and so on, associated with PS, occurring during the average follow-up of 17 months (range, 3-35months). Conclusions Our preliminary results revealed that anatomical lung resection by UVATS is a safe and feasible mini-invasive technique for PS patients, which might be associated with less postoperative pain, reduced paresthesia, better cosmetic results, and faster recovery.