bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNAs and their regulatory networks in breast muscle tissue of Chinese Gushi chicken during postnatal late development
Authors: Li Yuanfang , Chen Yi , Jin Wenjiao , Zhai Bin , Li Guoxi , Zhang Yanhua , Guo Yujie , Sun Guirong , Han Ruili , Li Zhuanjian , Li Hong , Tian Yadong , Liu Xiaojun , Kang Xiangtao 
Published: March 2020

Abstract
Background: Chicken skeletal muscle is an important economic product. Late stages of chicken development are the main period that affects meat production. LncRNAs play important roles in controlling the epigenetic process of growth and development. However, studies on the role of lncRNAs in the late stages of chicken breast muscle development are still lacking. In this study, in order to study the expression characteristics of lncRNAs during chicken muscle development, 12 cDNA libraries were constructed from Gushi chicken breast muscle samples from 6-, 14-, 22-, and 30-week-old chickens. Results: Therefore, a total of 1,252 new lncRNAs and 1,376 annotated lncRNAs were identified. Furthermore, 53, 61, 50, 153, 117, and 78 DE-lncRNAs were found in the W14 vs. W6, W22 vs. W14, W22 vs. W6, W30 vs. W6, W30 vs. W14 , and W30 vs. W22 comparison groups, respectively. After GO enrichment analysis of the DE-lncRNAs, several muscle development-related GO terms were found in the W22 vs. W14 comparison group. Furthermore, it was found that the MAPK signaling pathway was one of the most frequently enriched pathways in the different comparison groups. In addition, 12 common target DE-miRNAs of DE-lncRNAs were found in different comparison groups, some of which were muscle-specific miRNAs, such as gga-miR-206, gga-miR-1a-3p, and miR-133a-3p. Interestingly, the precursors of four newly identified miRNAs were found to be homologous to lncRNAs. Furthermore, we found some ceRNA networks associated with muscle development-related GO terms. For example, the ceRNA networks containing the DYNLL2 gene with 12 lncRNAs that targeted 2 miRNAs. In addition, we also constructed PPI networks, such as IGF-I - EGF and FZD6 - WNT11 , etc. Conclusions: This study for the first time revealed the dynamic changes of lncRNA expression in Gushi chicken breast muscle at different periods, and reveal the MAPK signaling pathway play a vital role in muscle development. Furthermore, MEF2C and its target lncRNA may be involved in muscle regulation through the MAPK signaling pathway. This research provided valuable resources for elucidating post-transcriptional regulatory mechanisms for postpartum development of chicken breast muscle.
The Age Factor Influencing Long Term Physical Functionality in Stroke Patients Undergoing Intra-arterial Thrombectomy Treatment
Authors: Lu Henry Horng-Shing , Kao Chi-Ling , Lin Chih-Ming , Chang Shu-Wei , Liu Chi-Kuang , Ou Yang-Hao 
Published: March 2020

Abstract
Background The treatment of acute ischemic stroke is heavily time-dependent, and even though, with the most efficient treatment, the long-term functional outcome is still highly variable. In this current study, the authors selected acute ischemic stroke patients who were qualified for intravenous thrombolysis with recombinant tissue plasminogen activator and followed by intra-arterial thrombectomy. With primary outcome defined by the functional level in a one-year follow-up, we hypothesize that patients with older age are at a disadvantage in post-stroke recovery. However, an age-threshold should be determined to help clinicians in selection of patients to undergo such therapy. Methods This is a retrospective chart review study that include 92 stroke patients in Changhua Christian hospital with a total of 68 evaluation indexes recorded. The current study utilized the forward stepwise regression model whose Adj-R2 and p-value in search of important variables for outcome prediction. The chngpt package in R indicated the threshold point of the age factor directing the better future functionality of the stroke patients. Results Datasets revealed the threshold of the age set at 79 the most appropriate. Admission Barthel Index, Age, Ipsi ICA RI, Ipsi VA PI, Contra MCA stenosis, Contra ECA RI, and in-hospital pneumonia are the significant predicting variables. The higher the age, in-hospital pneumonia, Contra MCA stenosis, Ipsi ICA RI and Ipsi VA PI, the less likely patient to recover from functional deficits as the result of acute ischemic stroke; the higher the value of Contra ECA RI and Admission Barthel Index, the better chance to recover at one-year follow up. Conclusions Parameters of pre-intervention datasets could provide important information to aid first-line clinicians in decision making. Especially, in patients whose age is above seventy-nine receives diminish return in the benefit to undergo such intervention and should be considered seriously by both the patients and the physicians.
Chitosan capping enzyme-responsive hollow mesoporous silica nanoplatforms for colon specific drug delivery
Authors: Cai Defu , Han Cuiyan , Liu Chang , Ma Xiaoxing , Qian Jiayi , Zhou Jianwen , Li Yue , Sun Yiming , Zhang Changting , Zhu Wenquan 
Published: March 2020

Abstract
In summary, an enzyme-responsive colon specific drug delivery system was developed based on hollow mesoporous silica sphere (HMSS), in which the biodegradable chitosan (CS) was gated on the openings of HMSS through cleavable azo bonds (HMSS-N=N-CS). Doxorubicin (DOX) was encapsulated into the hollow cavity and mesopores of HMSS, and HMSS-N=N-CS/DOX showed a high loading amount of 35.2%. X-ray diffraction (XRD) experiment proved that the DOX loaded in the HMSS-N=N-CS was in a non–crystalline state. In vitro drug release experiments proved that HMSS-N=N-CS/DOX showed an enzyme-responsive drug release property. The grafted CS could increase the biocompatibility and stability of HMSS, and reduce the protein adsorption on the surface of HMSS. The gastrointestinal mucosa irritation and cell cytotoxicity results indicated the good biocompatibility of HMSS and HMSS-N=N-CS. The confocal laser scanning microscope (CLSM) and flow cytometry technique (FCM) results indicated that the cellular uptake of DOX was obviously increased after the HMSS-N=N-CS/DOX was preincubated with colonic enzyme mixture. Cell viability result indicated that HMSS-N=N-CS/DOX incubated with colon enzyme showed an increased cytotoxicity and the IC50 value was three time less than that of HMSS-N=N-CS/DOX group. The present work will lay the foundation for subsequent research on mesoporous carriers for oral colon-specific drug delivery.
The Relationship between Clinical Dishonesty and Perceived Clinical Stress among Nursing Students in Southeast of Iran
Authors: Rafati Foozieh , Bagherian Behnaz , Mangolian Parvin , Imani-Goghary Zahra 
Published: March 2020

Abstract
Background Clinical dishonesty is a complex problem that threatens the health and safety of patients. This study aimed to investigate the relationship between clinical dishonesty and perceived clinical stress in nursing students. Method This cross sectional correlational study was performed on 395 nursing students from 4 nursing colleges. Data were collected using a demographic information questionnaire, Nursing Student's Perception of Clinical Stressors, and a 12-item researcher-made questionnaire to evaluate the frequency of clinical dishonesty in the past semester, the frequency of witnessing dishonest behavior among peers, and the perceived severity of behavior as unethical behavior. Results In this study, 89.1% of the students had at least one dishonest clinical behavior in the last semester. Frequency of clinical dishonesty was correlated with gender, interest in the field of study, academic year, frequency of observing dishonesty among peers, perceived severity of behaviors as unethical behavior, and perceived stress of students in the clinical setting. Conclusion The frequency of clinical dishonesty among nursing students is high and needs attention. Also, considering the positive relationship between dishonesty and perceived clinical stress, it is essential to teach effective strategies to nursing students to empower them to cope with clinical stress.
Strategies to improve the implementation of nurse-initiated management of antiretroviral therapy (NIMART) training: a systematic review
Authors: Mboweni Sheillah Hlamalani , MAKHADO LUFUNO 
Published: March 2020

Abstract
Background Nurse-Initiated Management of Antiretroviral Therapy ( NIMART) training was introduced with the purpose of improving the knowledge, skills and competence of nurses in providing comprehensive quality care to people living with HIV (PLWH) and facilitating World Health Organisation task-shifting where nurses initiate antiretroviral therapy (ART) rather than doctors to meet the increasing demand of ART drugs. The aim of this review was to identify evidence of training strategies that can improve the implementation and deals with challenges that hinder quality. Methods A measurement tool to assess systematic reviews methodological systematic review tool was used to critically assess selected studies. Studies were sought using multiple databases including CINAHL (EBSCO), Google Scholar, ProQuest, academic journals, SA publications, electronic resources and OAISTER published from January 2012 to February 2017. The initial review of studies yielded 535 results excluding duplications. The screening of full-text articles was conducted, and 33 articles met the eligibility criteria and were included for quality assessment. The rest were excluded. The quality assessment research instrument was used to analyse and synthesise selected full-text articles (n=33). Results The studies indicate that NIMART or HIV training is used interchangeably and has the potential to empower nurses with knowledge and skills. It also has a positive impact on increasing ART uptake; however, nurses still lack confidence and competence to provide comprehensive quality patient-centred care. Studies suggest that a shift from the traditional didactics of lectures or slide presentation methods during training to the use of interactive training strategies that stimulate critical thinking in making decisions about HIV care, followed by mentoring and continuous professional development. Conclusion and recommendation This study reveals that the use of effective training techniques during NIMART training has a positive impact on the learning outcomes, implementation and performance of HIV programmes. The health care system should focus on dealing with barriers that negatively influence NIMART implementation. Given the lack of a framework or model related to NIMART or HIV training, the study recommends the development of a conceptual framework to strengthens the implementation of NIMART training.
Gut microbiome is associated with thyroid nodule and functions
Authors: Ding Suying , Li Ang , Li Tiantian , Gao Xinxin , Yan Hang , Wang Yu , Chen Jingfeng , Jiao Mengfan , Ren Zhigang , Shi Qingmiao , Huang Meng , Wang Lin , Li Jingmei , Dong Yihan , Jia Zhuqing , Zhang Lihua , Feng Qing , Han Huandi , Yang Xiang , Li Yang , Rao Benchen , Yin Detao , Li Hongqiang , Ma Runsheng , Zeng Qiang , Yu Zujiang 
Published: March 2020

Abstract
Background: Thyroid nodule affects nearly half of the adult population. Accumulating evidence suggests that gut microbiota plays an important role in the thyroid disorders and functions, yet the association between gut microbiota capacity and thyroid nodule and function have not been studied comprehensively.Methods: We performed a gut microbiota whole-genome wide association study in 537 thyroid nodule participants and 725 controls from the general Chinese population. Thyroid nodules were with the Thyroid Imaging Reporting and Data System assessment.Results: Participants with the highest grade thyroid nodule had decreased gut microbial species and functional richness compared with controls and thyroid nodule with lower grade. Microbiota composition of the low-grade thyroid nodule was similar to that in controls rather than high-grade thyroid nodule. We found significant alternations of overall microbial functional composition in high-grade thyroid nodule but not the species composition, only a small portion of microbioal species were associated with high-grade thyroid nodule. The high-grade thyroid nodule gut microbiome was characterized by greater amino acid degradation and glycolysis, also with fewer Butyrivibrio and lower butyric acid production. Thyroid functions were positively associated with L-histidine and L-tyrosine related biosynthesis pathways, which are essential components of thyrotropin-releasing hormone and thyroxine, also corelated with broad nutrition metabolism pathways including fatty acids, B family vitamins and nucleotides. High-grade thyroid nodule discrimination models based on the gut microbiome and demography were validated using 294 independent samples and showed stable classification power.Conclusion: Our study comprehensively described the gut microbiome characteristics in thyroid nodule and suggested potiental role of the key gut microbiota-driven functional pathways in thyroid nodule and functions metabolism.
Guidelines of the Association of Anesthesiologists-Intensivists, the Interregional Non-Governmental Organization Alliance of Clinical Chemotherapists and Microbiologists, the Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy (IACMAC), and NGO Russian Sepsis Forum Diagnostics and antimicrobial therapy of the infections caused by multiresistant microorganisms
Authors: Beloborodov V. B. , Gusarov V. G. , Dekhnich A. V. , Zamyatin M. N. , Zubareva N. A. , Zyryanov S. K. , Kamyshova D. A. , Klimko N. N. , Kozlov R. S. , Kulabukhov V. V. , Polushin Yu. S. , Rudnov V. A. , Sidorenko S. V. , Shlyk I. V. , Edelshtein M. V. , Yakovlev S. V. 
Published: March 2020

Introduction.Strains of microorganisms resistant to antimicrobial agents are commonly found in medical units throughout most regions of the world, including Russia. This leads to lower antimicrobial therapy efficacy when treating nosocomial infections. In this regard, the timely implementation of adequate antibiotic therapy is of great importance.The objective of the guidelines:To provide summarized information on contemporary approaches to microbiological diagnostics and the assessment of results, as well as the principles of rational use of antimicrobial and antifungal agents, including treatment of infections caused by multiple drug-resistant strains of microorganisms.Subjects and methods.These guidelines are based on published data obtained in the course of randomized trials, as well as information presented in the provisions of international guidelines supported by high-level evidence. The guidelines were prepared by a working group of Russian experts with extensive experience in research and practical work in this area. On October 11, 2019, the ﬁnal version of the guidelines was reviewed and approved at a joint meeting of the working group and representatives of the public organizations which initiated the development of these guidelines (Association of Anesthesiologists-Intensivists, the Interregional Non-Governmental Organization Alliance of Clinical Chemotherapists and Microbiologists, the Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy (IACMAC), NGO Russian Sepsis Forum).Conclusion.The guidelines reﬂect an interdisciplinary consensus of approaches to the diagnostics and antibiotic therapy of infections caused by multiresistant microorganisms. The provisions set forth should be used to decide on the strategy of empirical and etiotropic therapy of the most severe infections.
Characterization and anaerobic digestion of highly concentrated Mexican wine by-products and effluents
Authors: Vital-Jacome M. , Cazares-Granillo M. , Carrillo-Reyes J. , Buitron G. 
Published: March 2020

Abstract
Wine production has increased in recent years, especially in developing countries such as Mexico. This increase is followed by an increase of winery effluents that must be treated to avoid environmental risks. However, little information is available about the characteristics of these effluents and the possible treatments. This paper aimed to characterize the effluents and by-products generated by the Mexican winery industry and to evaluate the performance and stability of the anaerobic treatment using a single-stage and a two-stage process. Results showed that the winery effluents had a high content of biodegradable organic matter, with chemical oxygen demand (COD) values ranging from 221 to 436 g COD/L. The single-stage anaerobic process was able to treat an organic loading rate of 9.6 kg COD/(m3 d); however, it was unstable and highly dependent on the addition of bicarbonate alkalinity (0.31 g NaHCO3/g COD removed). The two-stage process was more stable working at a higher organic load (12.1 kg COD/(m3 d)) and was less dependent on the addition of bicarbonate (0.17 g NaHCO3/g COD removed). The results highlight the potential of the winery effluents to produce methane through anaerobic digestion in a two-stage process, making wine production more sustainable.
Avaliação da Contaminação Ambiental por Arsênio e Estudo Epidemiológico da Exposição Humana em Paracatu-MG - Brasil
Authors: Castilhos Zuleica Carmen , De Capitani Eduardo Mello , Jesus Iracina Maura de , Bidone Edison Dausacker , Mello William Zamboni de , Lima Marcelo de Oliveira , Faial Kleber Raimundo Freitas , Mataveli Lidiane Raquel Verola , Arauz Luciana Juncioni de , Silva Lilian Irene Dias da , Ferreira Aldo Pacheco , Távora Renata Souza , Mertens Frédéric 
Published: March 2020

Apresentamos um estudo interdisciplinar onde condições ambientais, sociais e de saúde são integradas para avaliar o risco de exposição ao arsênio na população de Paracatu, devido à exploração da maior mina de ouro a céu aberto do Brasil. O estudo possui três fases: avaliação da contaminação ambiental; estudo epidemiológico de exposição ambiental humana; exame dos fatores associados aos comportamentos para reduzir o risco de exposição. As águas de consumo humano na área urbana não estão contaminadas com arsênio. Os teores de arsênio em urina indicam exposição mais alta em bairros próximos à mina, em concordância com os dados sobre material particulado atmosférico. A taxa de mortalidade por câncer não se mostrou superior aos controles. As redes sociais e relações de gênero são chave na adoção de comportamentos preventivos. Recomenda-se o estabelecimento de um Programa de Vigilância da exposição ao arsênio, com envolvimento do SUS, ao nível municipal, estadual e federal.
Non-Enzymatic Protein Acetylation by 7-Acetoxy-4-Methylcoumarin: Implications in Protein Biochemistry
Authors: Bhatt Anant Narayan , Rai Yogesh , Verma Amit , Pandey Sanjay , Kaushik Kumar , Parmar Virinder S. , Arya Anu , Prasad Ashok K. , Dwarakanath Bilikere S. 
Published: March 2020

Background:
The semi-synthetic acetoxycoumarins are known to acetylate proteins
using novel enzymatic Calreticulin Transacetylase (CRTAase) system in cells. However, the nonenzymatic
protein acetylation by polyphenolic acetates is not known.

Objective:
To investigate the ability of 7-acetoxy-4-methyl coumarin (7-AMC) to acetylate proteins
non-enzymatically in the test tube.

Methods:
We incubated 7-AMC with BSA and analyzed the protein acetylation using Western blot
technique. Further, BSA induced biophysical changes in the spectroscopic properties of 7-AMC
was analyzed using Fluorescence spectroscopy.

Results:
Using pan anti-acetyl lysine antibody, herein we demonstrate that 7-AMC acetylates
Bovine Serum Albumin (BSA) in time and concentration dependent manner in the absence of any
enzyme. 7-AMC is a relatively less fluorescent molecule compared to the parental compound, 7-
hydroxy-4-methylcoumarin (7-HMC), however the fluorescence of 7-AMC increased by two fold
on incubation with BSA, depending on the time of incubation and concentration of BSA. Analysis
of the reaction mixture of 7-AMC and BSA after filtration revealed that the increased fluorescence
is associated with the compound of lower molecular weight in the filtrate and not residual BSA,
suggesting that the less fluorescent 7-AMC undergoes self-hydrolysis in the presence of protein to
give highly fluorescent parental molecule 7-HMC and acetate ion in polar solvent (phosphate
buffered saline, PBS). The protein augmented conversion of 7-AMC to 7-HMC was found to be
linearly related to the protein concentration.

Conclusion:
Thus protein acetylation induced by 7-AMC could also be non-enzymatic in nature
and this molecule can be exploited for quantification of proteins.
Synthesis, Characterization and Anti-hepatoma Activity of New Hederagenin Derivatives
Authors: Liu Xiao , Sun Lu , Liu Qing-Hua , Chen Bao-Quan , Liu Yu-Ming 
Published: March 2020

Background:
Based on the biological significance of hederagenin-type saponins found in
our previous investigation, a series of new hederagenin derivatives were designed and synthesized.

Methods :
Their in vitro antiproliferative activities were evaluated against the HepG2 liver cancer cell
line and normal cell line L929 by MTT assay.

Results:
The preliminary bioassay results demonstrated that all the tested compounds 1-7 showed potent
anti-hepatoma activities, and some compounds exhibited better effects than 5-fluorouracil against
human hepatocellular carcinoma HepG2 cell line. Furthermore, compound 5 showed a significant antihepatoma
activity against HepG2 cells with an IC50 value of 1.88 µM. Besides, all of the tested compounds
showed a low cytotoxic effect against the normal cell line L929.

Conclusion:
All the compounds 1-7 displayed superior selectivity against human hepatocellular carcinoma
HepG2 cell line, and the results suggest that the structural modifications of C ring on the hederagenin
backbone are vital for modulating anti-hepatoma activities.
Liraglutide as Adjunct to Insulin Treatment in Patients with Type 1 Diabetes: A Systematic Review and Meta-analysis
Authors: Dimitrios Patoulias , Michael Doumas , Vasilios Kotsis , Konstantinos Stavropoulos , Konstantinos Imprialos , Ioanna Zografou , Konstantinos Petidis , Spyridon Bakatselos , Asterios Karagiannis 
Published: March 2020

Background:A few Randomized Controlled Trials (RCTs) have evaluated the use of liraglutide in Type 1 Diabetes (T1D). Through the present systematic review and meta-analysis, we aim at critically appraising and summarizing those RCTs, providing precise effect estimates.Methods:We searched major databases and grey literature from their inception to October 2018, for RCTs with a duration ≥ 12 weeks, comparing liraglutide with placebo or any other comparator as adjunct to insulin in patients with T1D, investigating major efficacy and safety endpoints. This review is reported in accordance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) statement.Results:We included 5 trials with 2,445 randomized participants. Liraglutide provided modest reductions in HbA1c, with liraglutide 1.8 mg producing the greatest decrease (MD = -0.24%, 95% CI -0.32 to -0.16, I2=0%). Significant weight reduction, up to 4.87 kg with liraglutide 1.8 mg was also observed (95% CI -5.31 to -4.43, I2=0%). Decrease in total daily insulin dose, primarily driven by a decrease in bolus insulin requirements, was demonstrated. Liraglutide decreased non-significantly the odds for severe hypoglycemia (OR=0.80, 95% CI 0.57-1.14, I2=0%), while it increased significantly the odds for gastrointestinal adverse events (for nausea, OR=4.70, 95% CI 3.68-6.00, I2=37%, and for vomiting, OR=2.50, 95% CI 1.54-4.72, I2=27%). A significant increase in heart rate was also demonstrated. No association with diabetic ketoacidosis or malignancies was identified.Conclusion:In patients with T1D, liraglutide might prove be an adjunct to insulin, improving glycemic control, inducing body weight loss and decreasing exogenous insulin requirements and severe hypoglycemia.