bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementasi Akuntabilitas Dalam Konsep Metafora Amanah di Lembaga Bisnis Syariah (Studi Kasus : Swalayan Pamella Yogyakarta)
Authors: Kalbarini Rahmah Yulisa , Suprayogi Noven 
Published: October 2020

This study is aimed to find out accountability within Trustful metaphor concept and know the process also type of accountability in islamic business organization case study at Pamella Supermarket.The methods of this study is qualitative approach using research study. The analyze technique is domain and taxonomy. Data is collected by interview, passive participant observation, and secondary data. The informants of this research are an assistant manager, a marketing officer, an employee and costumers of the Pamella Swalayan in Yogyakarta.The results obtained, that the implementation of accountability with trustful metaphor concept in Pamella Supermarket based on pamella owner’s concept on accountability that the purpose of life is rahmatan lil alamin that is each muslim can give a benefit to of the muslims. The realization of that concept is there will be a separation of responsibility : business fund from social fund which is managed by Pamella. From the supermarket sight, the business fund is under the responsibility of the owner and to Allah SWT. While the social fund is under the responsibility of the society by holding some social humanity program, which are reported to the society as well as to the existing interrelated departments in Yogyakarta.
Efektivitas Memberdayakan Ekonomi Pada Program Komunitas Usaha Mikro Muamalat Berbasis Masjid di Masjid Miftahul Jannah Surabaya
Authors: Ardiansyah Hanif , Nafik HR Muhammad 
Published: October 2020

The porpose of this research is to find out the effectiveness of Mosque-basedMicrobusiness Community Program to empowering mustahiq’s economy at Miftahul Jannah Mosque Surabaya.The research method of this research is a qualitative approach, case studies strategy, and descriptive analysis techniques which collecting data through interview, and observation. The informants are members of Mosque-based Microbusiness Community Program at Miftahul Jannah Mosque Surabaya, consist of seven people who had attanded the program and has recieved assistance zakat productive in the form venture capital from Baitul Maal Muamalat through Mosque-based Microbusiness Community Program (KUM3).Based on the results of the research is Mosque-based Microbusiness Community Program effective to empower mustahiq’s economy use productive zakat consist of venture capital, the mosque has a maximum role to facilitate the distribution of productive zakat from Baitul Maal Muamalat to mustahiq evidenced by increasing three indicators, namely ruhiyah, partisipation, and business.
Analysis on influencing factors of viral load in patients with high-risk human papillomavirus
Authors: Lu Xuerong , Wang Tiantian , Zhang Youzhong , Liu Yuzhen 
Published: October 2020

Abstract
Background: High-risk human papillomavirus (HR-HPV) load is thought to be influenced by many factors, and relationship between viral load and degree of cervical lesion is controversial. This study was to explore possible influencing factors of HR-HPV viral load in uterine cervix.Methods: 605 women who needed colposcopic evaluation for abnormal cervical screening in Affiliated Hospital of Weifang Medical University, China, between November 2017 and September 2018 were enrolled. Cervical specimens were collected from endo- and ectocervix separately using two different cervical brushes. Hybrid Capture II test was used to measure HR-HPV load. Age, histological severity, number of viral types, area and location of cervical lesions were recorded. The correlation between viral load and influencing factors was analysed using univariate and multivariate analysis.Results: HR-HPV load was positively correlated with age, histological severity, multiple HPV types and area of cervical lesions (P<0.05). Viral load with combination of endo- and ectocervical sampling was significantly higher than simple endocervical sampling (P<0.001). Multivariate analysis showed that age, multiple HPV types and area of cervical lesions were independent factors for HR-HPV load with combination of endo- and ectocervical sampling (P<0.05). However, only age and area of cervical lesions were independent factors for viral load with simple endocervical sampling (P<0.05). No significant association was found between viral load and lesion severity in multivariate analysis (P>0.05).Conclusion: HR-HPV load is influenced by age, histological severity, multiple viral types, area of cervical lesion and sampling methods. Age and area of cervical lesions are independent factors for viral load.
Consistency between Optical Coherence Tomography and Humphrey Visual Field for Evaluating Glaucomatous Defects in High Myopic Eyes
Authors: Wen Wen , Zhang Yuqiu , Zhang Ting , Sun Xinghuai 
Published: October 2020

Abstract
Background. The study is to investigate the influence of high myopia on the consistency between optical coherence tomography (OCT) and visual field in primary open-angle glaucoma (POAG). Methods. We enrolled 37 patients with POAG with high myopia (POAG-HM group), 27 patients with POAG without high myopia (POAG group), and 29 controls with high myopia (HM group). All subjects underwent Humphrey perimetry (30-2 and 10-2 algorithms). The peripapillary retinal nerve fiber layer (RNFL) and macular ganglion cell-inner plexiform layer (GCIPL) thicknesses were measured using Cirrus HD-OCT. Spearman’s rank correlation analysis was used to determine correlations between OCT and perimetric parameters. Agreement was analyzed by cross-classification and weighted κ statistics. Results. In POAG group, the cross-classification analysis showed strong agreement between the inferior temporal GCIPL thickness and 10-2 MS (κ = 0.5447, P = 0.0048), and good agreement between the superior and inferior RNFL thicknesses and 30-2 MS (κ = 0.4407 and 0.4815; P < 0.05). In the POAG-HM group, only the inferior temporal GCIPL thickness showed good agreement with 10-2 MS (κ = 0.3155, P = 0.0289). Conclusions. In POAG patients with high myopia, changes in macular measurements were in accordance with visual field defects, and RNFL thickness did not consistently decline with visual field defects due to the effects of high myopia. This study suggests that during diagnosis and follow-up of glaucoma with high myopia, more attention need to be focused on structure and functional defects in macular areas.
Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine
Authors: Zhao Fujie , Liu Lintao , Xu Menglong , Shu Xiangli , zheng Lanlan , Wei Zhanyong 
Published: October 2020

Abstract
Background: Background:Transmissible gastroenteritis virus (TGEV) causes enteric infection in piglets, characterized by vomiting, severe diarrhea and dehydration, and the mortality in suckling piglets is often high up to 100%. Vaccination is an effective measure to control the disease caused by TGEV. Methods: In this study, cell-cultured TGEV HN-2012 strain was inactivated by formaldehyde (FA), β-propiolactone (BPL) or binaryethylenimine (BEI), respectively. Then the inactivated TGEV vaccine was prepared with freund's adjuvant, and the immunization effects were evaluated in mice. The TGEV-specific IgG level was detected by ELISA. The positive rates of CD4+, CD8+, CD4+IFN-γ+, CD4+IL-4+ T lymphocytes were detected by flow cytometry assay. Lymphocyte proliferation assay and gross pathology and histopathology examination were also performed to assess the three different inactivating reagents in formulating TGEV vaccine.Results: The results showed that the TGEV-specific IgG level in FA group (n=17) was earlier and stronger, while the BEI group produced much longer-term IgG level. The lymphocyte proliferation test demonstrated that the BEI group had a stronger ability to induce spleen lymphocyte proliferation. The positive rates of CD4+ and CD8+ T lymphocyte subsets of peripheral blood lymphocyte in BEI group was higher than that in FA group and BPL groups by flow cytometry assay. The positive rate of CD4+IFN-γ+ T lymphocyte subset was the highest in the BPL group, and the positive rate of CD4+IL-4+ T lymphocyte subset was the highest in the FA group. There were no obvious pathological changes in the vaccinated mice and the control group after the macroscopic and histopathological examination. Conclusions: These results indicated that all the three experimental groups could induce cellular and humoral immunity, and the FA group had the best humoral immunity effect, while the BEI group showed its excellent cellular immunity effect.
Diminished adherence of Biomphalaria glabrata embryonic cell line to sporocysts of Schistosoma mansoni following programmed knockout of the allograft inflammatory factor
Authors: Coelho Fernanda Sales , Rodpai Rutchanee , Miller Andre , Karinshak Shannon E , Mann Victoria H , Carvalho Omar dos Santos , Cladeira Roberta Lima , Mourao Marina de Moraes , Brindley Paul J , Ittiprasert Wannaporn 
Published: October 2020

Abstract
Background: Larval development in an intermediate host gastropod snail of the genus Biomphalaria is an obligatory component of the life-cycle of Schistosoma mansoni. Understanding of the mechanism(s) of host defense may hasten the development of tools that block transmission of schistosomiasis. The allograft inflammatory factor 1, AIF, which is evolutionarily conserved and expressed in phagocytes, is a marker of macrophage activation in both mammals and invertebrates. AIF enhances cell proliferation and migration. The embryonic cell line, termed Bge, from Biomphalaria glabrata is a versatile resource for investigation of the snail-schistosome relationship since Bge exhibits a hemocyte-like phenotype. Hemocytes perform central roles in innate and cellular immunity in gastropods and in some cases can kill the parasite. However, the Bge cells do not kill the parasite in vitro.Methods: Bge cells were transfected by electroporation with plasmid pCas-BgAIFx4, encoding the Cas9 nuclease and a guide RNA specific for exon 4 of the B. glabrata AIF (BgAIF) gene. Transcript levels for Cas9 and for BgAIF were monitored by reverse-transcription-PCR and, in parallel, adhesion of gene-edited Bge cells during co-culture with of schistosome sporocysts was assessed.Results: Gene knockout manipulation induced gene-disrupting indels, frequently 1–2 bp insertions and/or 8–30 bp deletions, at the programmed target site; a range from 9 to 17% of the copies of the BgAIF gene in the Bge population of cells were mutated. Transcript levels for BgAIF were reduced by up to 73% (49.5 ± 20.2% SD, P ≤ 0.05, n =12). Adherence by BgAIF gene-edited (ΔBgAIF) Bge to sporocysts diminished in comparison to wild type cells, although cell morphology did not change. Specifically, as scored by a semi-quantitative cell adherence index (CAI), fewer ΔBgAIF than control wild type cells adhered to sporocysts; control CAI, 2.66 ± 0.10, ΔBgAIF, 2.30 ± 0.22 (P ≤ 0.01).Conclusions: The findings supported the hypothesis that BgAIF plays a role in the adherence of B. glabrata hemocytes to sporocysts during schistosome infection in vitro. This demonstration of the activity of programmed gene editing will enable functional genomics approaches using CRISPR/Cas9 to investigate additional components of the snail-schistosome host-parasite relationship.
Correlation between sestrin2 expression and airway remodeling in COPD
Authors: Zhang Da-Wei , Wei Yuan-Yuan , Ji Shuang , Fei Guang-He 
Published: October 2020

Abstract
Background. Airway remodeling is a major pathological characteristic of chronic obstructive pulmonary disease (COPD), and has been shown to be associated with oxidative stress. Sestrin2 has recently drawn attention as an important antioxidant protein. However, the underlying correlation between sestrin2 and airway remodeling in COPD has yet to be clarified.Methods. A total of 124 subjects were enrolled in this study, including 62 control subjects and 62 COPD patients. The pathological changes in airway tissues were assessed by different staining methods. The expression of sestrin2 and matrix metalloproteinase 9 (MMP9) in airway tissues was monitored by immunohistochemistry. Enzyme-linked immunosorbent assays (ELISAs) were used to detect the serum concentrations of sestrin2 and MMP9. The airway parameters on computed tomography (CT) from all participants were measured for evaluating airway remodeling. The relationship between serum sestrin2 and MMP9 concentration and airway parameters in chest CT was also analyzed. Results. In patients with COPD, staining of airway structures showed distinct pathological changes of remodeling, including cilia cluttered, subepithelial fibrosis, and reticular basement membrane (Rbm) fragmentation. Compared with control subjects, the expression of sestrin2 and MMP9 was significantly increased in both human airway tissues and serum. Typical imaging characteristics of airway remodeling and increased airway parameters were also found by chest CT. Additionally, serum sestrin2 concentration was positively correlated with serum MMP9 concentration and airway parameters in chest CT.Conclusion. Increased expression of sestrin2 is related to airway remodeling in COPD. We demonstrated for the first time that sestrin2 may be a novel biomarker for airway remodeling in patients with COPD.
Human Reproduction is Regulated by Retrotransposons derived from Ancient Hominin-Specific Viral Infections
Authors: Tao Yu , Xiang Xiinyu , Hsu Fei-Man , Pontis Julien , Trono Didier , Liu Wanlu , Clark Amander 
Published: October 2020

Abstract
Germ cells are essential to pass DNA from one generation to the next. In human reproduction, germ cell development begins with the specification of primordial germ cells (PGCs) and a failure to specify PGCs leads to human infertility. Recent studies have revealed that the transcription factor network required for PGC specification has diverged in mammals, and this has a significant impact on our understanding of human reproduction. Here, we evaluated the emerging epigenetic landscape during hPGC specification using a combination of in vivo and in vitro analysis of hPGCs/hPGC-like cells (hPGCLCs) and human embryonic stem cells (hESCs). Our data reveals that hominid restricted Transposable Elements (TEs) partly derived from ancient viruses are pre-bound by the transcription factors TFAP2C and NANOG in undifferentiated hESCs, become transcriptionally induced during PGC specification and undergo dynamic epigenetic reprogramming leading to increased chromatin accessibility, localized DNA demethylation and establishment of broad peaks of H3K27ac. Using KRAB mediated CRISPRi we show that blocking this remodeling has a significant impact on hPGC specification. In summary, our data reveals that human reproduction requires the establishment of an epigenetic landscape during hPGC specification driven by the acquisition of hominid-specific TEs that were derived from ancient viral infections that entered the hominid germline less than 5 million years ago.
Reversible formation of coordination bonds in Sn-based metal-organic frameworks for high-performance lithium storage
Authors: Liu Jingwei , Xie Daixi , Xu Xiufang , Jiang Luozhen , Si Rui , Shi Wei , Cheng Peng 
Published: October 2020

Abstract
Sn-based compounds with buffer matrixes possessing high theoretical capacity, low working voltage, and alleviation of the volume expansion of Sn are ideal materials for lithium storage. However, it is highly challenging to confine well-dispersed Sn within a lithium active matrix because low-melting-point Sn tends to agglomerate. Here, for the first time, we apply a metal-organic framework (MOF) chemistry between Sn-nodes and lithium active ligands to create two Sn-based MOFs comprising Sn2(dobdc) and Sn2(dobpdc) with extended ligands from H4dobdc (2,5-dioxido-1,4-benzenedicarboxylate acid) to H4dobpdc (4,4’-dioxidobiphenyl-3,3’-dicarboxylate acid) with molecule-level homodispersion of Sn in organic matrixes for lithium storage. The enhanced utilization of active sites and reaction kinetics are achieved by the isoreticular expansion of the organic linkers. The reversible formation of coordination bonds during lithium storage processes is first revealed by X-ray absorption fine structure characterization, providing an in-depth understanding of the lithium storage mechanism in coordination compounds.
Therapeutic Effects of Genetically Modified Wharton’s Jelly Mesenchymal Stem Cells Expressing Erythropoietin on Cancer-related Anemia in Mice Model
Authors: Estiri Mohammad , Estiri Bahareh , Fallah Asghar , Aghazadeh Marzyeh , Sedaqati Amir , Abdollahi Abbas , Rabienia Mahsa , Mortazavidehkordi Nahid , Farjadfar Akbar 
Published: October 2020

Abstract
BackgroundCancer-related anemia (CRA) negatively influences cancer patients’ survival, disease progression, treatment efficacy, and quality of life (QOL). Current treatments such as iron therapy, red cell transfusion, and erythropoietin-stimulating agents (ESAs) may cause severe adverse effects. Therefore, development of long-lasting and curative therapies is highly required. Combined cell and gene therapy platform can introduce a new route for permanent production of erythropoietin (EPO) in the body with various degrees of clinical benefits and avoiding the need for repeat treatments.MethodsIn this study, we developed cell and gene therapy strategy for in-vivo delivery of EPO cDNA via genetic engineering human Wharton’s jelly mesenchymal stem cells (hWJMSCs) to long-term produce and secret human EPO protein after transplantation into the mice model of CRA. To evaluate CRA's treatment in cancer-free and cancerous conditions, at first, we designed recombinant breast cancer cell line 4T1 expressing herpes simplex virus type 1 thymidine kinase (HSV1-TK) by a lentiviral vector encoding HSV1-TK and injected into mice. After 3 weeks, all mice develop the metastatic breast cancer associated to the acute anemia. Then, we administrated ganciclovir (GCV) for 10 days in half of the mice to clear cancer cells. Meanwhile, we designed another lentiviral vector encoding EPO to transduce hWJMSCs. Following implantation of rhWJMSCs-EPO, the whole peripheral blood samples were collected from the tail vein once per week which were immediately analyzed for the measurements of EPO, hemoglobin (Hb), and hematocrit (Hct) plasma levels. Results We found that after implantation of rhWJMSCs-EPO, plasma EPO, Hb, and Hct concentration significantly increased which rose to a peak in the fourth week and remained at a therapeutic level for >17 weeks in the cancer-free group and >10 weeks in the cancerous group.ConclusionOur data indicate that the EPO-transduced hWJMSCs could improve the anemia of cancer in both cancer-free and cancerous mice model. This significant difference in length of time that Hb and Hct are in therapeutic levels in both treatment groups indicates that developing a precise targeted-therapy to eliminate cancer cells along with an effective treatment for CRA, as we presented here, could bring important clinical benefits.
Quantifying the Effects of Post-surgery Recovery Time on the Migration Dynamics and Survival Rates in the Wild of Acoustically Tagged Atlantic Salmon Salmo Salar Smolts
Authors: Daniels Jason , Brunsdon Eric B. , Chaput Gerald , Dixon Heather , Labadie Holly , Carr Jonathan W. 
Published: October 2020

Abstract
Background: The experimental effects of surgically implanting fish with acoustic transmitters is likely to have negative effects on survival and behavior. Measuring the extent of these negative effects is important if we wish to extrapolate inferences from tagged animals to unmanipulated animals. In this study, we examine the effect of surgery and post-tagging recovery time on the survival and migration rate of acoustically tagged wild Atlantic Salmon (Salmo salar) smolts through freshwater, estuarine and ocean phases of migration. Four treatment groups were used: pre-smolt captured in the fall that overwintered in a hatchery and were tagged either 75 days prior to release (winter hatchery) or within 24 hours prior to release (spring hatchery) and smolt captured during the spring smolt run, tagged 24 hours prior to release and released during the day (day-released) or night (night-released).Results: The spring hatchery treatment group served as a reference treatment group such that surgical (comparison to winter hatchery treatment) and hatchery effects (comparison to day-released and night-released treatments) could both be discerned. The hatchery effect increased migration rate whereas short recovery times and captivity in a hatchery negatively affected survival. These effects were most pronounced within the first 5 days and/or 48 km downstream post-release, however, the residual surgical effects appeared to persist during the transition from the estuary into salt water.Conclusions: Even with smolts originating from the wild and spending relatively little time within the hatchery environment, post-release survival was still negatively affected. Migration speed was faster for hatchery smolts but is likely only due to their larger size. Surgical effects were most prominent during the initial migration period in freshwater and again in the transition from the estuary to saltwater which may be due to added stress during these transitional zones. As surgery related bias will likely never be completely removed from telemetry studies it is important to quantify and account for these effects in situ when making inferences on the unmanipulated component of the population.
The effects of Fe and NH4Cl on silicon nitridation
Authors: Yi X.M. , Zhang Senjing , Zhu Weijun , Li Qingda , Zhang Wenjie , Li Songze 
Published: October 2020

Abstract
Silicon nitride (Si3N4) with a high α-phase content is in demand due to its higher thermal conductivity for use in heat dissipation bases for electronic devices. However, more needs to be understood about its fibrous growth, therefore, rich fibrous morphologies were synthesized with the assistance of Fe powder and NH4Cl. The effects of these additives on the phase content and fiber morphologies of the products were investigated The results illustrate that the Si3N4 products possess upper and lower layers and that when the Fe powder content is 4 wt% and NH4Cl content is 5 wt%, the Si3N4 fibers have smooth surfaces, uniform diameters, and no floating particles. Moreover, the maximum α-Si3N4 content reached 94.8 wt%, demonstrating an increase in this phase. The double mechanisms of vapor-liquid-solid and vapor-solid are presented as the growth mechanism at the fiber.