bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dipyridamole-thallium scanning doesn't reduce 90-day cardiac complications and mortality in femoral neck fracture patients undergoing hemiarthroplasty: a propensity-score-matched analysis in an Asian population
Authors: Liao Chin-Yi , Tan Timothy L , Lu Yu-Der , Wu Cheng-Ta , Kuo Feng-Chih , Lee Mel S 
Published: March 2020

Abstract
Background: This study aimed to evaluate the effect of dipyridamole-thallium scanning on 90-day cardiac complications and mortality in patients with femoral neck fracture treated with hemiarthroplasty.Methods: Between 2008 and 2015, 844 consecutive patients undergoing cemented or cementless hemiarthroplasty were identified from the database of a single level-one medical center. One-hundred and thirteen patients (13.4%) underwent the dipyridamole-thallium scan test prior to surgery, and 731 patients (86.6%) did not. Patient characteristics, comorbidities, surgical variables, and duration to surgery were recorded. Multivariate logistic regression was performed, and a propensity-score-matched cohort was identified in order to determine whether dipyridamole-thallium scanning was associated with 90-day cardiac complications and mortality.Results: The incidence of 90-day cardiac complications was 19.47% in the thallium scan group and 11.58% in the non-thallium scan group (p = 0.343) among 452 patients after propensity score-matching. The 90-day mortality rate was similar in the two groups (0.88% vs. 1.18%, p = 0.795). In the propensity score-matched patients, utilization of the dipyridamole-thallium scan was irrelevant to cardiac complications (matched cohort, adjusted odds ratio [OR] = 1.32; 95% confidence interval [CI] 0.75 to 2.33) or mortality (adjusted OR = 0.75; 95% CI 0.08 to 6.71) within 90 days. Risk factors for cardiac complications were an American Society of Anesthesiologists grade 3 (OR 3.19, 95% CI 1.44 to 7.08) and pre-existing cardiac comorbidities (OR 5.56, 95% CI 3.35 to 9.25). Discussion: Preoperative dipyridamole-thallium scanning was not associated with reductions in 90-day cardiac complications and mortality in patients with femoral neck fracture undergoing hemiarthroplasty. The necessity for dipyridamole-thallium scanning should be re-evaluated in elderly patients with femoral neck fractures, given that this increases the duration to surgery.
Comparison between subcutaneous pocket and cryopreservation method for storing autologous bone flaps in developing bone flap infection after cranioplasty, a prospective observational multi-center study of 100 cases
Authors: Shafiei Mehdi , Sourani Arman , Korki Amir Arsalan , Mahram Saeed 
Published: March 2020

Abstract
Background Following a craniotomy, harvested bone flap, if available, is stored for future cranioplasty. There are two different methods for bone banking: subcutaneous pocket in abdominal wall and cryopreservation in a refrigerator. This study is designed to evaluate risk of developing infection in each group, retrospectively. Methods In this prospective observational multi-center study, one hundred (100) patients who underwent cranioplasty with autologous bone flap were divided into two groups of 50 patients. rate of clinical post-operative infection and possible associated risk factors were analyzed with statistical measures. The data check lists parameters were indication of DC, CP vs SP, interval between craniotomy and cranioplasty, post-operative hospitalization duration, new morbidities and possible mortalities. The data were analyzed by an expert bio-statistician with proper bio-statistical methods (p<0.05) Results Four (4) patient in cryopreservation group (50 patients) had post-operative bone flap infection (8%).in subcutaneous pocket method no post-operative infection after cranioplasty was noticed, which are not statistically significant (p=0.054). over-all post-operative infection rate was 4%. Conclusion There were no significant differences in post-operative infection rate between cryopreservation versus subcutaneous pocket method in storing bone flaps. Older age maybe associated with infection development after cranioplasty.
A prognostic nomogram based on competing endogenous RNA network for clear-cell renal cell carcinoma
Authors: Peng Yun , Wu Shangrong , Xu Zihan , Hou Dingkun , Li Nan , Zhang Zheyu , Wang Lili , Zhang Yangyi , Wang Haitao 
Published: March 2020

Abstract
Backgroud Clear-cell renal cell carcinoma (ccRCC) is stubborn to traditional chemotherapy and radiation treatment, which makes its clinical management a major challenge. Recently, we have made efforts to understand the etiology of ccRCC. Increasing evidence revealed that the competing endogenous RNA (ceRNA) were involved in the development of various tumor. However, it’s scant for studying on ccRCC, and a comprehensive analysis of prognostic model based on lncRNA-miRNA-mRNA ceRNA regulatory network of ccRCC with large-scale sample size and RNA‐sequencing expression data is still limited. Methods RNA‐sequencing expression data were taken out from GTEx database and TCGA database, A total of 354 samples with ccRCC and 157 normal controlled samples were included in our study. The ccRCC-specific genes were obtained from WGCNA and differential expression analysis. Following, the communication between mRNAs and lncRNAs and target miRNAs were predicted by MiRcode, starBase, miRTarBase, and TargetScan. A gene signature of eight genes was constructed by univariate Cox regression, lasso methods and multivariate Cox regression analysis. Results A total of 2191 mRNAs and 1377 lncRNAs was identified, and a dys-regulated ceRNA network for ccRCC was established using 7 mRNAs, 363 lncRNAs, and 3 miRNAs. Further, a gene signature in cluding 8 genes based on this ceRNA was constructed, meanwhile, a nomogram predicting 1-, 3-, 5-year survival probability containing both clinical characteristics and ccRCC-specific gene signatures was developed. Conclusion It could contribute to a better understanding of ccRCC tumorigenesis mechanism and guide clinicians to make a more accurate treatment decision.
Vertebrae rotation made little difference on the position of abdomial aorta relative to spine in adult degenerative scoliosis
Authors: Liang Yan , Xu Shuai , Zhu Zhenqi , Liu Haiying , Meng Fanqi 
Published: March 2020

Abstract
Background: Few study has been published for quantitative relation of the vertebrae rotation on the position of the aorta relative to spine in adult degenerative scoliosis (ADS). Thus, this study was to explore the effect of the vertebrae rotation on the position of the abdomial aorta relative to spine in patients with ADS and identify the risk factor of vertebrae rotation.Methods: A retrospective analysis was performed for 71 patients with ADS divided into left scoliosis (LS group, 40cases) and right scoliosis (RS group, 31cases). The two group were well-matched in demographics. Apical vertebrae, Cobb angle (°), coronal displacement and thoracolumbar kyphosis (TLK) were measured on X-ray. Aorta-vertebrae angle (α), aorta-vertebrae distance (d) and rotation angle (γ) for each level of T12-L4 on MRI were obtained within a Cartesian coordinate system.Result: There was no significant difference in apical vertebrae distribution between the LS and RS group, so were Cobb angle and coronal horizontal displacement distance (P˃0.05). There was no significance on mean γ of of apical vertebrae among different levels in LS group and RS group (P ˃0.05). Pearson correlation analysis showed γ was a positively correlated to Cobb angle and coronal horizontal displacement distance (P<0.001) in both groups. There was no significant correlation between the γ and α, d, whichever the group (P˃0.05). Coronal movement was the independent risk factor of γ in LS group with the equation of γ = -4.502+0.542×Coronal movement while Cobb angle and coronal horizontal movement were risk factors in RS group with γ = 2.953-0.512×Cobb angle -0.406×Coronal movement.Conclusion: Vertebral rotation could be predicted by Cobb angle or coronal movement by X-ray in ADS and aorta maintained a relatively normal position in patients with ADS.
Cloning and functional analysis of JnCYCD3;1 in Jatropha nigroviensrugosus
Authors: Feng Xiao , Yang Zhao , Xiu-Rong Wang 
Published: March 2020

Abstract
Background: As a new variety of Jatropha, the female to male flower ratio and yield of Jatropha nigroviensrugosus are higher than the common J. curcas. Using pre-transcriptome data, the full-length gene sequence, subcellular function localization, and verification of the transgenic function were obtained in order to understand the specific functions of differentially expressed genes (DEGs). Results: Results revealed that the open reading frame (ORF) of J. nigroviensrugosus CYCD3;1 (JnCYCD3;1) was 414 bp long, encoding 137 amino acids (aa). Compared to J. curcas, the presence of intron retention led to early termination of the coding frame. JnCYCD3;1 had the highest expression in new leaves, which was 68.42 times root expression, followed by inflorescence buds. The JnMYC2 ORF was 2025 bp, encoding 674 aa. JnMYC2 had the highest expression levels in inflorescence buds. JnCYCD3;1 functioned in the nucleus, while JnMYC2 was distributed in both the nucleus and cytoplasm and may possess transmembrane membrane behavior. Bimolecular fluorescence complementation (BIFC) experiments indicated that JnMYC2 interacted with JnCYCD3;1. JnCYCD3;1 transgenic tobacco considerably advanced the reproductive cycle and may promote flower formation and transformation. Conclusions:The related experiments obtained new CYCD3;1 transcript, verified that CYCD3;1 is related to flower bud differentiation, proved the interaction between hormones-related genes. The study provides a new research direction for gene function of CYCD3;1.
The molecular basis of loss of smell in 2019-nCoV infected individuals
Authors: Gupta Krishan , Mohanty Sanjay Kumar , Kalra Siddhant , Mittal Aayushi , Mishra Tripti , Ahuja Jatin , Sengupta Debarka , Ahuja Gaurav 
Published: March 2020

Abstract
Among the prominent clinical symptoms such as fatigue, shortness of breath, fever, and cough, 2019-nCoV infected individuals often experience hyposmia/anosmia (decrease or loss of sense of smell). Angiotensin I Converting Enzyme 2 (ACE2), a key host receptor has now been established as an important moiety for the entry of 2019-nCoV into the host cells. A multitude of studies estimated the expression of ACE2 in multiple organs including heart, kidney, intestines, lungs, buccal cavity, etc. The ongoing medical examinations and the autopsy reports of the diseased individuals strongly corroborate these organ/tissue-level molecular insights. Olfactory mucosa harbors multiple functionally distinct cell types. Zeroing in on the cell lineages that underpin infection associated loss of olfaction may provide new leads for diagnostics/clinical management of 2019-nCoV infected individuals. Our pointed bioinformatic analysis of single-cell expression profiles underscored selective expression of ACE2 in a subset of horizontal basal cells (HBCs) and sustentacular cells (SUSs) of the olfactory mucosa in humans. Inspection of the ACE2 levels in the olfactory mucosa of 4 additional mammalian species revealed comparable expression patterns, indicating the risk of olfactory dysfunction in these species. In summary, our findings pinpoint the molecular rationale of loss of smell in 2019-nCoV infected patients.
ASCUS Negative Conversion in Pelvic Floor Reconstruction of POP Patients with Human Papillomavirus-Negative ASCUS Cytology
Authors: Wei Ai-Min , Fan Yun , Shen Yu-Fei , Zhang Lei , Tan Xiao-Mei 
Published: March 2020

Abstract
Objective To inquire into the influence of the pelvic floor reconstruction surgery of pelvic organ prolapse on Papillomavirus-Negative atypical squamous cells of undetermined significance cytology. Methods This investigation was studied retrospectively about female patients of POP 3~4 stage with high-risk human papillomavirus DNA-negative ASCUS between January, 2008 and September, 2018.All patients received one of two treatment regimens, pelvic floor reconstruction or local hydrocortisone cream(0.1% Hydrocortisone Butyrate cream, 1g nightly for no less than 12 weeks). After 6 months follow-up, we collected data to compare the positive rate of ASCUS with high-risk human papillomavirus DNA-negative between the two groups after intervention. Results The negative conversion rate of ASCUS in the operative patients was higher than in patients using local hydrocortisone cream (33/47 [70.2%] vs 19/44 [43.2%], P = 0.009). Conclusions The decline of non–human papillomavirus ASCUS cytology in postoperative patients might be related to pelvic floor reconstruction.
CRISPR/Cas9-mediated microRNA-21 Cleavage Sensitizes the Treatment Response to Dasatinib in Chronic Myeloid Leukemia Cell Line K562
Authors: Zhang Yun , Wang Lingyan , Li Jiazheng , Jiang Peifang , Lin Minhui , Hu Jianda , Chen Buyuan 
Published: March 2020

Abstract
Background: Tyrosine kinase inhibitors (TKIs) targeting BCR-ABL1 kinase are effective in treating chronic myeloid leukemia (CML), but TKI resistance occurs in a significant number of patients, and the underlying molecular mechanisms of this resistance remain largely unknown. As an oncomiR, microRNA-21(miR-21) functions directly in drug resistance, but its relationship with TKI resistance in CML is rarely reported. As a novel and effective gene editing tool, clustered regularly interspaced short palindromic repeats–CRISPR-associated protein 9(CRISPR/Cas9) has certain advantages in completely knocking out target genes at the gene level. Methods: We successfully constructed lentiviruses LV-VMP1-sgRNA (045001) and human chronic myeloid leukemia K562 cells were transducted with them. Single-cell-derived clones were screened for miR-21 deletion by genomic DNA PCR and Sanger sequence. RQ-PCR assays was used to confirm the knockout of miR-21. 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide(MTT) and colony formation assays were applied to assess the cell growth inhibition. Imatinib and dasatinib sensitivity was determined by MTT assay and Annexin-V APC/7-AAD double staining flow cytometry. Western blot assay was performed to measure the levels of Phosphatase and tensin homolog(PTEN), Phosphatidylinositol 3-kinase(PI3K), Serine/threonine Kinase(AKT), p-AKT, BCR-ABL(P210), p-BCR-ABL (p-P210) . Result: miR-21 knockout inhibited proliferation of K562 cells, promoted their apoptosis and increased their sensitivity to dasatinib. Further mechanism studies suggest that this is achieved by inhibiting the PI3K/AKT signaling pathway and destroying BCR-ABL. Conclusions: Our study reveals the efficacy of CRISPR/Cas9 gene editing to miR-21 in K562 and indicates miR-21 as a potential target in sensitizing dasatinib treatment for CML patients with pool response to the TKI targeting therapy.
Effects of StAAP1 and StAAP8 in Potato ATFs Transcription Factor Family on Plant Development
Authors: Zhang Chao , Kong Nana , Si JiaXin , Wang Dongdong , Chen Qin 
Published: March 2020

Abstract
Background: Amino acid transporters mediated the transport of various amino acids in plants, which were essential for plant growth and development. ATFs (the amino acid transporter family) was one of amino acid transporters in plants and AAPs (amino acid permease) subfamily belongs to the ATFs family.Results: In this research, eight AAP genes were identified in the potato and divided into two subgroups. The first subgroup is the StAAP gene containing six microporous transport channels (Pore-lining), and the second subgroup are the StAAP gene containing seven microporous transport channels. The subcellular location and overexpression vectors were built by StAAP1 and StAAP8. The results indicated these two genes were localized on the cell membrane. Observing the phenotype of the transgenic plants, StAAP1 and StAAP8 promoted the growth of leaves, StAAP1 changed the shape of tubers and enhanced their weight and StAAP8 was no significant influence in tubers but decreased the weight a little.Conclusions: In this study, the expression pattern showed StAAP8 might regulate the transport of amino acids in potato roots and affect the osmotic potential of potato root cells. Thereby, the tolerance of plants to abiotic stress and StAAP1 and StAAP8 promote the amino acid transport into leaves and StAAP1 had effected on tuber development and StAAP8 might resist the weight of tubers.
The impact of COVID-19 on ischemic stroke: A case report
Authors: Zhai Pan , Ding Yanbing , Li Yiming 
Published: March 2020

Abstract
BackgroundThe outbreak of a novel coronavirus since December 2019 in Wuhan, became an emergency of major international concern. As of March 5, 2020, the SARS-CoV-2 epidemic has caused 80,565 confirmed infections with 3,015 fatal cases in China. The SARS-CoV-2 outbreak is a major challenge for clinicians. In our clinic, we found a rare case that a COVID-19 patient combined with ischemic stroke.Case PresentationA 79-year-old man was admitted to the Hubei Provincial Hospital of Chinese Traditional Medicine due to right limb weakness for 1 day and slight cough for 1 week. At presentation, his body temperature was 37.3°C (99.0°F) with some moist rales. Neurological examination showed right limb weakness, and the limb muscle strength was grade 4. The left leg and arms were unaffected. In addition, runs of speech were not fluent enough with tongue deviation. Laboratory studies showed lymphopenia and eosinophilic granulocytopenia. Chest CT revealed bilateral pulmonary parenchymal ground-glass and consolidative pulmonary opacities, with a peripheral lung distribution. Real-time polymerase chain reaction (RT-PCR) from throat swab sample was positive for SARS-CoV-2 nucleic acid. This patient was treated with antiviral drugs and anti-inflammatory drugs with supportive care until his discharge. Clopidogrel (75 mg) and atorvastatin (20 mg) were administered orally to treat acute ischemic stroke. After twelve days of treatment, he can walk normally and communicate with near fluent language.ConclusionWe report an even more unusual case, a patient who was hospitalized for right limb weakness and was later diagnosed with COVID-19. Here, SARS-CoV-2 infection caused hypoxemia and excessive secretion of inflammatory cytokines, which contribute to the occurrence and development of ischemic stroke. Once COVID-19 patients show acute ischemic stroke, neurologists should cooperate with infectious disease doctors to help patients.
Outbreak Measures Taken by Medical Schools During the Coronavirus Pandemic in London, United Kingdom: A Qualitative Study
Authors: Ting Andrew Ser Jien , Ho Carmen Lok Tung 
Published: March 2020

Abstract
Background: Coronavirus disease (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was first reported in Wuhan, China in December 2019. On 11 March 2020, the World Health Organization (WHO) declared COVID-19 to be a pandemic. As of mid-March 2020, more than a total of 150,000 cases have been reported in 122 countries, including 1,543 in the United Kingdom. Within London, there are five universities with medical schools each faced with difficult decisions on how to respond to this unprecedented situation, having to balance the education of future doctors who will soon be joining the front lines and their safety. In this paper, the responses and timeliness of medical schools are collated and compared. This will help guide medical schools’ responses in the future.Methods: Information was gathered from the official university websites and social media platforms. Thematic analysis was performed to obtain overarching categories of responses by the medical schools.Results: All five medical schools displayed similar responses to COVID-19, following guidance provided by Public Health England (PHE), Foreign & Commonwealth Office (FCO) and Medical Schools Council (MSC). Eight broad themes of responses were identified to have been undertaken by most London medical schools. Responses such as suspending clinical placements, keeping university facilities open and not banning on-campus events were universally adopted by all five medical schools. Other responses such as specific exam rearrangements and elective travel advice were more heterogeneous amongst the medical schools.Conclusion: Medical schools must take extraordinary measures in response to a pandemic. The experience gained from the COVID-19 pandemic will help future administrations be more confident in providing a more rapid response to similar health crises.
Plan of tests with addition. Efficient estimate of dependability indicators
Authors: Mikhailov V. S. 
Published: March 2020

The Aim of the paper consists in improving the efficiency of dependability indicator estimation for the plan of tests with addition, i.e. probability of no-failure and mean time to failure. Due to economic considerations, determinative dependability tests of highly dependable and costly products involve minimal numbers of products, expecting failure-free testing or testing with one failure, thus minimizing the number of tested products. The latter case is most interesting. By selecting specific values of the acceptance number Q and number of tested products, the tester performs a preliminary estimation of the dependability indicator, while selecting Q = 1 the tester minimizes the risks caused by an unlikely random failure. However, as the value Q grows, the number of tested products does so as well, which makes the testing costly. Therefore, the reduction of the number of products tested for dependability is the firstpriory problem and, in this context, economic planning of testing with addition is becoming increasingly important. We will consider binomial tests (original sample) with addition of one product (oversampling) to testing in case of failure of any of the initially submitted products. Testing ends when all submitted products have been tested with any outcome (original sampling and oversampling). Hereinafter it is understood that the testing time is identical for all products. Testing with the acceptance number of failures greater than zero (Q > 0) conducted with addition allows reducing the number of tested products through successful testing of the original sample.Methods. Efficient estimation is based on the integral approach formulated in many papers. The integral approach is based on the formulation of the rule of efficient estimate selection specified on the vertical sum of absolute (or relative) biases of estimates selected out of a certain set based on the distribution law parameter, where n is the number of products initially submitted to testing. The criterion of selection of an efficient estimate of the probability of failure (or PNF) at a set of estimates is based on the total square of absolute (or relative) biases of the mathematical expectation of estimates from probability of failure p for all possible values of p, n.Conclusions. The paper examines the probability of no-failure estimates for the plan of tests with addition. For the case of n > 3, the estimates and composite estimate are more efficient in comparison with estimate . The composite estimate of the probability of no-failure should be used in failure-free tests. For the case of n > 3, testing with the acceptance number of failures greater than zero (Q > 0) conducted with addition allows reducing the number of tested products through successful testing of the original sample. The composite estimate of the mean time to failure is bias-efficient among the proposed mean time to failure estimates. The obtained composite estimates and are of practical significance in the context of failure-free testing with addition.