bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pilot study for cystic fibrosis neonatal screening: the Cuban experience
Authors: Castells Elisa M. , Sánchez Aramis , Frómeta Amarilys , Mokdse Yanin , Ozunas Nelson , Licourt Tania , Arteaga Ana Luisa , Silva Eladio , Collazo Teresa , Rodríguez Fidel , Martín Odalys , Espinosa Maryeris , del Río Lesley , Pérez Pedro L. , Morejón Greilys , Almira Claudia , Núñez Zoe , Melchor Antonio , González Ernesto Carlos 
Published: May 2020

Abstract

Background
In Cuba, no screening program for cystic fibrosis (CF) has been implemented yet. The ultramicro enzyme-linked immunosorbent assay (UMELISA)® TIR NEONATAL has been developed for the measurement of immunoreactive trypsin (IRT) in dried blood spots on filter paper. The analytical performance of the kit was evaluated in the national network of laboratories.

Methods
Newborn dried blood samples (DBS) were evaluated in 16 laboratories. An IRT/IRT/DNA protocol was followed using a cut-off value of 50 ng/mL. The mean, median and percentiles of the distribution were calculated and a two-sample t-test with unequal variance was used for statistical analysis. Influence of perinatal factors on IRT levels was analyzed.

Results
From January to June 2018, 6470 newborns were studied, obtaining a mean IRT value of 12.09 ng/mL (ranging 0–358 ng/mL) and a median of 8.99 ng/mL. Fifty-two samples (0.78%) were above the cut-off level and 16 samples (0.24%) were elevated in the re-screening process. One of them was confirmed positive by molecular biology (phe508del/c.3120 + 1G > A), constituting the first newborn screened and diagnosed early in Cuba. Second DBS samples were collected on average at 14 days and processed in the laboratory at 16 days of birth. Significant differences were observed (p < 0.05) when evaluating the influence of gender, birth weight (BW) and gestational age (GA) on the IRT values. Lower IRT concentrations were found in samples processed after 10 days of collection.

Conclusions
The performance of UMELISA® TIR NEONATAL in the laboratories has been satisfactory; hence CF newborn screening (NBS) was extended throughout the country from January 2019.
Faecal haemoglobin distributions by sex, age, deprivation and geographical region: consequences for colorectal cancer screening strategies
Authors: Clark Gavin R.C. , Strachan Judith A. , McPherson Alisson , Digby Jayne , Mowat Craig , Steele Robert J.C. , Fraser Callum G. 
Published: May 2020

Abstract

Objectives
Faecal immunochemical tests for haemoglobin (FIT) are becoming widely used in colorectal cancer (CRC) screening and assessment of symptomatic patients. Faecal haemoglobin concentration (f-Hb) thresholds are used to guide subsequent investigation. We established the distributions of f-Hb in a large screening population by sex, age, deprivation and geography.

Methods
Single estimates of f-Hb were documented for all individuals participating in the first 18 months of the Scottish Bowel Screening Programme (SBoSP). The distributions of f-Hb were generated for all participants, all men and women, and men and women by age quintile and deprivation quintile. Distributions were also generated by geographical region for all participants, men and women, and by deprivation. Comparisons of f-Hb distributions with those found in a pilot evaluation of FIT and three other countries were performed.

Results
f-Hb was documented for 887,248 screening participants, 422,385 men and 464,863 women. f-Hb varied by sex, age, deprivation quintile and geographical region. The f-Hb distributions by sex and age differed between the SBoSP and the pilot evaluation and the three other countries.

Conclusions
f-Hb is higher in men than in women and increases with age and deprivation in both sexes. f-Hb also varies by geographical region, independently of deprivation, and by country. The f-Hb distribution estimated by pilot evaluation may not represent the population distribution. Decision limits have advantages over reference intervals. Use of partitioned f-Hb thresholds for further investigation, based on the data generated, has advantages and disadvantages, as do risk scores based on a spectrum of influencing variables.
The internal quality control in the traceability era
Authors: Braga Federica , Pasqualetti Sara , Aloisio Elena , Panteghini Mauro 
Published: May 2020

Abstract
To be accurate and equivalent, laboratory results should be traceable to higher-order references. Furthermore, their quality should fulfill acceptable measurement uncertainty (MU) as defined to fit the intended clinical use. With this aim, in vitro diagnostics (IVD) manufacturers should define a calibration hierarchy to assign traceable values to their system calibrators. Medical laboratories should know and verify how manufacturers have implemented the traceability of their calibrators and estimate the corresponding MU on clinical samples. Accordingly, the internal quality control (IQC) program should be redesigned to permit IVD traceability surveillance through the verification by medical laboratories that control materials, provided by the manufacturer as a part of measuring systems, are in the clinically suitable validation range (IQC component I). Separately, laboratories should also monitor the reliability of employed IVD measuring systems through the IQC component II, devoted to estimation of MU due to random effects and to obtaining MU of provided results, in order to apply prompt corrective actions if the performance is worsening when compared to appropriate analytical specifications, thus jeopardizing the clinical validity of test results.
Situativity: a family of social cognitive theories for understanding clinical reasoning and diagnostic error
Authors: Merkebu Jerusalem , Battistone Michael , McMains Kevin , McOwen Kathrine , Witkop Catherine , Konopasky Abigail , Torre Dario , Holmboe Eric , Durning Steven J. 
Published: May 2020

Abstract
The diagnostic error crisis suggests a shift in how we view clinical reasoning and may be vital for transforming how we view clinical encounters. Building upon the literature, we propose clinical reasoning and error are context-specific and proceed to advance a family of theories that represent a model outlining the complex interplay of physician, patient, and environmental factors driving clinical reasoning and error. These contemporary social cognitive theories (i.e. embedded cognition, ecological psychology, situated cognition, and distributed cognition) can emphasize the dynamic interactions occurring amongst participants in particular settings. The situational determinants that contribute to diagnostic error are also explored.
Vitamin D Supplementation Rescues Aberrant NF-κB Pathway Activation and Partially Ameliorates Rett Syndrome Phenotypes inMecp2Mutant Mice
Authors: Ribeiro Mayara C. , Moore Seth M. , Kishi Noriyuki , Macklis Jeffrey D. , MacDonald Jessica L. 
Published: May 2020

AbstractRett syndrome (RTT) is a severe, progressive X-linked neurodevelopmental disorder caused by mutations in the transcriptional regulatorMECP2. We previously identified aberrant NF-κB pathway upregulation in brains ofMecp2-null mice and demonstrated that genetically attenuating NF-κB rescues some characteristic neuronal RTT phenotypes. These results raised the intriguing question of whether NF-κB pathway inhibitors might provide a therapeutic avenue in RTT. Here, we investigate whether the known NF-κB pathway inhibitor vitamin D ameliorates neuronal phenotypes inMecp2-mutant mice. Vitamin D deficiency is prevalent among RTT patients, and we find thatMecp2-null mice similarly have significantly reduced 25(OH)D serum levels compared with wild-type littermates. We identify that vitamin D rescues aberrant NF-κB pathway activation and reduced neurite outgrowth ofMecp2knock-down cortical neuronsin vitro. Further, dietary supplementation with vitamin D in early symptomatic maleMecp2hemizygous null and femaleMecp2heterozygous mice ameliorates reduced neocortical dendritic morphology and soma size phenotypes and modestly improves reduced lifespan ofMecp2-nulls. These results elucidate fundamental neurobiology of RTT and provide foundation that NF-κB pathway inhibition might be a therapeutic target for RTT.
Role of Synaptic Inhibition in the Coupling of the Respiratory Rhythms that Underlie Eupnea and Sigh Behaviors
Authors: Borrus Daniel S. , Grover Cameron J. , Conradi Smith Gregory D. , Del Negro Christopher A. 
Published: May 2020

AbstractThe preBötzinger complex (preBötC) gives rise to two types of breathing behavior under normal physiological conditions: eupnea and sighing. Here, we examine the neural mechanisms that couple their underlying rhythms. We measured breathing in awake intact adult mice and recorded inspiratory rhythms from the preBötC in neonatal mouse brainstem slice preparations. We show previously undocumented variability in the temporal relationship between sigh breaths or bursts and their preceding eupneic breaths or inspiratory bursts. Investigating the synaptic mechanisms for this variabilityin vitro, we further show that pharmacological blockade of chloride-mediated synaptic inhibition strengthens inspiratory-to-sigh temporal coupling. These findings contrast with previous literature, which suggested glycinergic inhibition linked sigh bursts to their preceding inspiratory bursts with minimal time intervals. Furthermore, we verify that pharmacological disinhibition did not alter the duration of the prolonged interval that follows a sigh burst before resumption of the inspiratory rhythm. These results demonstrate that synaptic inhibition does not enhance coupling between sighs and preceding inspiratory events or contribute to post-sigh apneas. Instead, we conclude that excitatory synaptic mechanisms coordinate inspiratory (eupnea) and sigh rhythms.
How Do We Segment Text? Two-Stage Chunking Operation in Reading
Authors: Yang (杨金骉) Jinbiao , Cai (蔡清) Qing , Tian (田兴) Xing 
Published: May 2020

AbstractChunking in language comprehension is a process that segments continuous linguistic input into smaller chunks that are in the reader’s mental lexicon. Effective chunking during reading facilitates disambiguation and enhances efficiency for comprehension. However, the chunking mechanisms remain elusive, especially in reading, given that information arrives simultaneously yet the written systems may not have explicit cues for labeling boundaries such as Chinese. What are the mechanisms of chunking that mediates the reading of the text that contains hierarchical information? We investigated this question by manipulating the lexical status of the chunks at distinct levels in four-character Chinese strings, including the two-character local chunk and four-character global chunk. Male and female human participants were asked to make lexical decisions on these strings in a behavioral experiment, followed by a passive reading task when their electroencephalography (EEG) was recorded. The behavioral results showed that the lexical decision time of lexicalized two-character local chunks was influenced by the lexical status of the four-character global chunk, but not vice versa, which indicated the processing of global chunks possessed priority over the local chunks. The EEG results revealed that familiar lexical chunks were detected simultaneously at both levels and further processed in a different temporal order, the onset of lexical access for the global chunks was earlier than that of local chunks. These consistent results suggest a two-stage operation for chunking in reading, the simultaneous detection of familiar lexical chunks at multiple levels around 100 ms followed by recognition of chunks with global precedence.
The Influence of Middle Eastern Tourists on Local Community in Cisarua Bogor in the Perspective of Identity Politics and Communitarian Citizenship
Authors: Ngenget Indiana , Indarwati Netik 
Published: May 2020

This research aimed to analyze the influence of Middle Eastern tourists on local community in Cisarua Bogor in the perspective of Identity Politics and Communitarian Citizenship. This research usedqualitative with a descriptive explanatory research type. Data collection techniques used were observation, in-depth interviews, and library research. The results of this research showed that Middle Eastern tourists positively encouraged tourism activities and economic development of local community but there was an emergence of social, political, and cultural problems such as identity crisis. Local community in Cisarua as part of Indonesia is faced with the presence of a minority group namely Arab tourists who visit this region at certain times and demand a recognition of their identity. So that the localcommunity in Cisarua is faced with multicultural life because they touch and interact with values that are different from what they have. Empirically, from a political perspective, Middle Eastern tourists have encouraged the formation of identity politics and the issue of communitarian citizenship while local values are increasingly eroded. The problem of communitarian citizenship is reflected in the phenomenon of Arabic villages through the rise of Arabic writing in business, the dominance of Arabic culture, Arabic food, and so on. The thick Middle Eastern culture in tourist areas becomes a problem when dealing with local culture and nationalism as a challenge for a sovereign nation. The novelty of this research is the influence of Middle Eastern tourists in the perspective of Political Science on identity politics and communitarian citizenship.
Follow The Money Approach in The Management of Fishing Criminals by Fisheries PPNS
Authors: Zakariya Rizki 
Published: May 2020

The many cases of Illegal Fishing, threaten the wealth of fisheries and sovereignty of Indonesia. This study aims to explain the urgency of the Follow the Money approach in handling fisheries crime cases by PPNS Fisheries. Then provide concrete recommendations for the Follow the Money implementation in handling fisheries crime cases. This paper is a normative juridical study, which is processed qualitatively. The results of this study indicate the urgency of the Follow the Money approach in handling fisheries crime cases by PPNS Fisheries due to Indonesia's large fishery wealth; the magnitude of the threat of illegal fishing in Indonesia; lack of fisheries crime cases handled by PPNS Fisheries; and the development of the modus operandi of fisheries criminals. Then, the efforts made in the Follow the Money approach are by increasing collaboration with PPATK institutions; increased cooperation with international law enforcement agencies; and the use of electronic transaction evidence as evidence at court.
Relationship Between the Initial Position of Palatally Displaced Canines and Treatment Duration
Authors: Esenlik Elçin , Bolat Esra , Findik Yavuz 
Published: May 2020

This prospective study aimed to assess the positional features of palatally displaced maxillary canines (PDCs), their relationship with both cephalometric and dental cast measurements and treatment duration. Pretreatment panoramic and lateral cephalometric radiographs and dental casts of 46 patients (23 patients with uni/bilateral PDC and 23 patients with Class I malocclusion) were collected. The mesial inclination of the permanent canine to the midline (α angle), the distance from the cusp tip of the permanent canine to the occlusal line (d distance), and the mesial position of the crown of the displaced canine (sector) were measured on the panoramic radiographs. SNA°, SNB°, ANB°, SN-GoGn°, SN-PP°, and PP-MP° angles and sagittal inclinations of the PDCs’ (C-PP°) were measured on cephalometric radiographs. Arch length discrepancies and transversal arch measurements were also performed. The Student’s t-test, Mann-Whitney U test and Kruskal-Wallis test were used to compare variables that were not normally distributed while ANOVA was used for the normally distributed data. The arch widths were similar between the groups while crowding was significantly greater in the PDC group. A negative correlation was found between the α angle and vertical plane angle (SN-GoGn°). Treatment duration was positively correlated with α angle and d distance but there was no relationship with the sagittal angulation of the PDC to the palatal plane (C-PP°) and the treatment duration. Treatment duration can be expected to be longer with every increase in the angle of the PDC to the midline and distance from the occlusal plane.
The effect of various classes of glucose-lowering medications on the blood vessel elasticity in patients with type 2 diabetes
Authors: Nedogoda S. V. , Barykina I. N. , Salasyuk A. S. , Sanina T. N. , Smirnova V. O. , Popova E. A. 
Published: May 2020

Aim. To study the effect of various classes of glucose-lowering agents (dipeptidyl peptidase-4 (DPP-4) and sodium-glucose cotransporter-2 (SGLT-2) inhibitors) on the vascular stiffness in patients with type 2 diabetes (T2D) and high cardiovascular risk.Material and methods. The open-label, prospective 24-week study included 120 patients with T2D and high cardiovascular risk. We evaluated the effect of modern glucose-lowering medications, empagliflozin at a dose of 25 mg/day (SGLT-2 inhibitor) and sitagliptin at a dose of 100 mg/day (DPP-4 inhibitor), on vascular elasticity, central hemodynamic and laboratory parameters.Results. After 24-week therapy, the target glycated hemoglobin level reached 71% and 80% of patients in the sitagliptin and empagliflozin groups, respectively. In both groups, vascular stiffness and central hemodynamic parameters were improved. However, significant changes were recorded only in the empagliflozin (carotid-femoral pulse wave velocity decreased by 14,4%, augmentation index — by 6%, central pulse pressure — by 7,8%) (p<0,05). Use of sitagliptin was associated with significant improvements in the lipid profile (total cholesterol decreased by 9,5%, triglycerides — by 21%, low density lipoproteins — by 15,1%; high density lipoproteins increased by 15,7%) (p<0,05). In the empagliflozin group, anthropometric parameters were improved (body mass index decreased by 9,1%, waist circumference — by 4,1%) (p<0,05). Patients in both groups had a significant decrease in HOMA-IR index and highly sensitive C-reactive protein: by 34% and 51,6% in the empagliflozin group and by 31,8% and 22,1% in the sitagliptin group, respectively (p<0,05).Conclusion. The use of empagliflozin is more associated with arterial stiffness decrease in T2D patients with high cardiovascular risk compared with sitagliptin.
Barriers to effective outpatient hypertension treatment: a view of physicians and patients
Authors: Nedogoda S. V. , Sabanov A. V. , Bychkova O. I. 
Published: May 2020

Aim. To study the opinion of primary care physicians and hypertensive (HTN) outpatients with different compliance rate on factors preventing effective antihypertensive therapy (AHT).Material and methods. Primary care physicians and HTN outpatients were questioned. Questionnaires for physicians and patients included informative and survey parts, with a list of possible factors aggravated adherence to treatment. The assessment was carried out using visual analogue scale. The patient questionnaire also included questions about AHT and the eight-item Morisky Medication Adherence Scale (MMAS-8). The calculation was carried out with a 95% confidence interval (CI).Results. The survey involved 298 physicians and 517 patients. Among patients, about 1% had a high compliance rate, 34% — moderate, 65% — low. In all groups, AHT did not significantly differ and was characterized by a low frequency of prescribing fixeddose combinations (27,1%). According to physicians, the most significant and equivalent are the economic aspects of treatment — 7,9±2,1 (95% CI: 7,51-8,38), the need for lifestyle change — 7,9±2,4 (95% CI: 7,37-8,38) and, to a slightly lesser extent, psychological aspects 6,8±2,2 (95% CI: 5,43-6,43). The economic aspects of treatment and need for lifestyle change were also most significant factors according to patients with high (8,8±1,8 (95% CI: 7,23-10,37) and 8,4±1,7 (95% CI: 6,93-9,87), respectively) and low (95% CI: 6,4±3,0 (5,65-7,07) and 6,2±2,8 (95% CI: 5,5-6,82) respectively) compliance rates. For patients with moderate compliance rate, the most significant and almost equivalent factors were the need for lifestyle change — 5,6±3,3 (95% CI: 4,53- 6,71), the need for regular visits — 5,6±3,1 (95% CI: 4,53-6,58) and the need for selfmanagement — 5,6±2,8 (95% CI: 4,63-6,48).Conclusion. The results obtained make it possible to forecast the compliance rate of patients with HTN, and, therefore, direct more efforts to those with a low rate, thereby increasing the effectiveness of AHT.
Outcomes in patients with hypertension and type 2 diabetes receiving a stent for angina
Authors: Akhtereyev R. N. , Galyavich A. S. , Baleeva L. V. , Galeeva Z. M. 
Published: May 2020

Aim. To study the outcomes in hypertensive (HTN) patients receiving a stent for class III angina, depending on concomitant type 2 diabetes (T2D).Material and methods. The study included 295 patients aged 45 to 75 years. All patients underwent coronary angiography followed by elective stenting. Clinical outcomes were evaluated after outpatient follow-up (average 44 months). Patients were divided into two groups: group 1 — 214 patients with coronary artery disease (CAD) in combination with HTN; group 2 — 81 patients with CAD in combination with HTN and T2D.Results.In the group of patients without T2D, during the follow-up period, class III angina was observed in 92 patients (43%). The decrease in systolic blood pressure (BP) (SBP) and diastolic BP (DBP) in this subgroup was 18 and 14 mm Hg, respectively (p<0,001); 35 patients (38%) had myocardial infarction (MI). In the group of patients with T2D, class III angina was recorded in 60 patients (74,1%). The decrease in SBP and DBP in this subgroup was 19 and 12 mm Hg, respectively (p<0,001). There were 58 cases of MI in this subgroup (96,7%).Conclusion.After stent insertion, patients with CAD, HTN and T2D still reporting class III angina were more likely to have MI than patients without T2D. This indicates a greater contribution of T2D to MI development, despite an equal decrease of SBP in patients with/without T2D.