bluuebunny/crossref_metadata_2025_split huggingface dataset:
The MicroRNA-130a-5p/RUNX2/STK32A Network Modulates Tumor Invasive and Metastatic Potential in Non-Small Cell Lung Cancer
Authors: Ma Fang , Xie Yangchun , Lei Yiyu , Kuang Zengshuyu , Liu Xianling 
Published: April 2020

Abstract
Background Non-small cell lung cancer (NSCLC) remains a huge health burden for human health and life worldwide. Our study here was to illuminate the relevance of microRNA-130a-5p (miR-130a-5p) on growth and epithelial mesenchymal transition (EMT) in NSCLC cells as along with metastasis in vivo, and to explore the underlying mechanism.Methods RT-qPCR was carried out for miR-130a-5p expression determination in NSCLC cells and tissue samples. Dual luciferase reporter gene assay, RT-qPCR and western blot were carried out to study the potential targets of miR-130a-3p. Effects of miR-130a-5p, runt-related transcription factor 2 (RUNX2) and encoding serine/threonine kinase 32A (STK32A) on NSCLC proliferation, migration, invasion as well as EMT processes were assessed by cell counting kits-8, colony formation, Transwell and western blot assays.Results miR-130a-5p was diminished in NSCLC tissues and cells versus their counterparts. miR-130a-5p exerted its repressive role in NSCLC by curtailing cell viability, migration, invasion as well as EMT, while facilitating apoptosis. miR-130a-5p directly targeted RUNX2, a transcription factor, and conversely regulated its expression. RUNX2 was found to interacted with STK32A to promote its expression. Following the validation of the tumor-supporting role of STK32A in NSCLC cells, RUNX2 overexpression was monitored to reverse miR-130a-5p-inhibited NSCLC tumor volume and weight through enhancing STK32A expression in vivo.Conclusions miR-130a-5p diminished the growth and EMT of NSCLC cells by regulating the RUNX2/STK32A axis, offering possible targets for the treatment for NSCLC.
Neutrophil-to-lymphocyte ratio is a prognostic factor for colon cancer: a propensity score analysis
Authors: Mazaki Junichi , katsumata Kenji , Kasahara Kenta , Tago Tomoya , Wada Takahiro , Kuwabara Hiroshi , Enomoto Masanobu , Ishizaki Tetsuo , Nagakawa Yuichi , Tsuchida Akihiko 
Published: April 2020

Abstract
Background: A large number of patients suffer recurrence after curative resection, and mortality from colon cancer remains high. The role of cancer-associated malnutrition such as the neutrophil-to-lymphocyte ratio (NLR) in cancer recurrence and death has been increasingly recognized. This study aimed to analyze long-term oncologic outcomes of Stage II-III colon cancer to examine the prognostic value of NLR using a propensity score analysis.Methods: A total of 442 patients with colon cancer underwent radical surgery between 2000 and 2014 at Tokyo Medical University Hospital. Long-term oncologic outcomes of these patients were evaluated according to NLR values. A cut-off NLR of 3.0 was used based on receiver operating characteristic curve analysis. A propensity score analysis according to tumor sidedness was also performed. Primary outcomes were overall survival (OS) and relapse-free survival (RFS). Results: Patients with lower NLR values (“lower NLR group”) were more likely to have lymph node metastasis compared to those with higher NLR values (“higher NLR group”) before case matching. After case matching, clinical outcomes were similar between the two groups. There were no significant difference in 5-year OS and 5-year RFS rates between the two groups before case matching based on propensity scores. After case matching, 5-year OS rates were 94.5% in the lower NLR group (n = 135) and 87.0% in the higher NLR group (n = 135), showing a significant difference (p = 0.042). Five-year RFS rates were 87.8% in the lower NLR group and 77.9% in the higher NLR group, also showing a significant difference (p = 0.032). Among patients with left-sided colon cancer, 5-year OS and 5-year RFS rates were 95.2% and 87.3% in the lower NLR group (n = 88), respectively, and 86.4% and 79.2% in the higher NLR group (n = 71), respectively, showing significant differences (p = 0.014 and p = 0.047, respectively).Conclusions: The NLR is an important prognostic factor for advanced colon cancer, especially for left-sided colon cancer.
The effectiveness of an online multimodal intervention for mental health promotion: A randomised controlled trial
Authors: Przybylko Geraldine , Morton Darren , Kent Lillian , Morton Jason , Hinze Jason , Beamish Peter , Renfrew Melanie 
Published: April 2020

Abstract
Introduction: There is an urgent need for efficacious interventions to combat the global mental health crisis, and mental health promotion and primary preventive approaches are paramount. Objective: This study examined whether an online multimodal intervention that incorporates evidence-based strategies from the disciplines of Lifestyle Medicine and Positive Psychology improved measures of mental health and emotional wellness. Methods: 425 adults (68.8% females, aged 46.5 ± 15.6) from Australia and New Zealand were randomised to an intervention or control group. The intervention group participated in a ten-week online multimodal intervention. Measures of mental health and emotional wellness were taken at baseline (Week 1), post-intervention (Week 12), and 12 weeks post-intervention (Week 24). The control group completed the same assessments. Results: The intervention group experienced significant improvements from Week 1 to 12 in: mental health (10%, p < 0.001, d = 0.50) and vitality (22%, p < 0.001, d = 0.54) subscales of the Short Form Survey (SF-36); depression (-42%, p < 0.001, d = 0.48), anxiety (-38%, p < 0.001, d = 0.39) and stress (-31%, p < 0.001, d = 0.52) subscales of the Depression, Anxiety and Stress Scale (DASS-21); and life satisfaction (8%, p < 0.001, d = 0.48) as measured by the Satisfaction With Life Scale (SWLS).The control group experienced significant improvements only in the mental health (3%, p = 0.028, d = 0.16) subscale of the SF-36, and the stress subscale (-9%, p = 0.038, d = 0.15) of the DASS-21. The changes in the intervention group were significantly greater (p < 0.001) than the control group for all measures. Improvements in the outcome measures were generally sustained for the intervention group at 12 weeks post-intervention. Conclusion: The online multimodal intervention improved measures of mental health and emotional wellness suggesting that such interventions may be useful for mental health promotion and prevention. Trial registration: The Australian New Zealand Clinical Trials Registry ACTRN12619000993190. Registered on 12 July 2019 (Retrospectively registered). The ANZCTRN is part of the WHO Primary Registries.
Polyurethane green composites for the treatment of boron present in the oil produced water
Authors: Syed Murtuza Ali , Sawafi Mohammed Al , Shaik Feroz 
Published: April 2020

Abstract
Polyurethane (PU) based algae biocomposite is synthesized and tested for boron removal from oil produced water. The percentage of algae is varied in the biocomposite and its physical and chemical properties are evaluated. The surface morphology, crystalline structure, thermal stability is characterized using scanning electron microscope, fourier transform infrared spectroscopy and thermal gravimetric analysis. The density of synthesized PU/algae composites is in the range of 1.12 and 1.20 g/ml based on content of filler in the PU matrix. Weight losses of the tested specimens in various chemical solutions are less than 10%. The boron removal efficiency is in the range of 84–85%, depending upon the algae filler at pH 7.19.
Long-term outcomes of gastric cancer in a single small center: a retrospective analysis
Authors: Jeong Ji Yoon , Kim Kwang Hee , Seo Sang Hyuk , An Min Sung , Baik HyungJoo , Park Yo-Han , Kang Sang Hyun , Kim Jin Soo , Kim Ki Hoon , Oh Sang Hoon 
Published: April 2020

Abstract
Background

The aim of this study is to evaluate the long-term outcomes and short-term outcomes of gastric cancer patients who underwent surgical treatment in a small-sized single center by one surgeon.
Methods

We retrospectively reviewed the medical records of 950 patients who underwent surgical treatment for gastric cancer between January 2000 and January 2010 at Dong-rae Paik Hospital, Inje University College of Medicine, Busan, Korea. A total of 820 patients were included and analyzed. We divided the time period into 2 groups: 2000–2005 (period 1) and 2006–2010 (period 2). Since all the surgeries were performed by one surgeon, we were able to analyze the changes in long-term outcomes as the individual surgeon volume accumulated.
Results

The mean follow-up period was 78.26 months. The overall survival and relapse-free survival rates of all patients were 84.6%, and 86.5%, respectively; the postoperative morbidity and mortality rates were 12% and 0.36%, respectively. The relapse-free survival rate was higher in period 2 than in period 1 (82.9% versus 90.2%, p = 0.002)
Conclusions

Our study showed that the short-term outcomes and long-term outcomes of gastric cancer treatment in a small-sized hospital were comparable to those in large-sized hospitals. Accumulated individual surgeon volume was related to a high relapse-free survival rate.
Immunoregulation of Microglia M2 polarization is an unrecognized physiological function of α -Synuclein
Authors: Li Na , Stewart Tessandra , Sheng Lifu , Shi Min , Cilento Eugene M , Wu Yufeng , Hong Jau-Syong , Zhang Jing 
Published: April 2020

Abstract
BACKGROUNDMicroglial function is vital for maintaining the health of the brain, and their activation is an essential component of neurodegeneration. It is increasingly recognized that microglia also undergo changes, dependent on the cellular environment, that promote mainly reconstructive and anti-inflammatory functions, i.e. mostly desirable functions of microglia in a physiological state. What maintains microglia at this physiological state is essentially unknown, despite significant research on factors that provoke “reactive” or “inflammatory” phenotypes in conditions of injury or disease. One such factor, exposure to the aggregated or oligomeric forms of α-synuclein, an abundant brain protein, plays an essential role in driving microglial activation; including chemotactic migration and production of inflammatory mediators in Lewy body (LB) diseases such as Parkinson’s disease.METHODSIn this study, using in vitro and in vivo models, we challenged primary microglia or BV2 microglia with LPS + IFN-γ,IL-4 + IL-13, α-synuclein monomer and α-synuclein oligomer, examined microglia phenotype and the underlying mechanism by RT-PCR, Western blot, ELISA, IF, IHC, Co-IP. RESULTS: We described a novel physiological function of α-synuclein, in which it modulates microglia towards an anti-inflammatory phenotype by interaction with extracellular signal-regulated kinase (ERK) and recruitment of the ERK, nuclear factor kappa B (NF-κB), and peroxisome proliferator-activated receptor γ (PPARγ) pathways. CONCLUSIONS: These findings suggest a previously unrecognized function of α-synuclein that likely gives new insights into the pathogenesis and potential therapies for Lewy body-related diseases and beyond, given the abundance and multiple functions of α-synuclein in brain tissue.
Kaempferol inhibits pseudorabies virus replication in vitro through regulation of MAPKs and NF-κB signaling pathways
Authors: Chen Xu , Chen Yaqin , Yin Zhongqiong , Wang Rui , Hu Huaiyue , Liang Xiaoxia , He Changliang , Yin Lizi , Zhang Wei , Ye Gang , Zou Yuanfeng , Lv Cheng , Li Lixia , Tang Huaqiao , Zhao Ling , Yue Guizhou , Jia Renyong , song xu 
Published: April 2020

Abstract
Pseudorabies virus (PRV), belonging to the family Herpesviridae, is a pathogen of Aujeszky’s disease leading great economic losses to pig industry. Re-outburst of pseudorabies implies that new control measures are urgent needed. The present study provides a candidate drug for PRV infection that kaempferol possesses the ability to inhibit PRV replication in a dose-dependent manner in vitro. Kaempferol at a concentration of 52.40 μM could decrease PRV-induced cell death by 90%. Kaempferol with a IC50 of 25.57μM is more effective than acyclovir (Positive control) with a IC 50 of 54.97 μM. Mode of action study indicated that kaempferol inhibited viral penetration and replication stages and virus load was decreased by 4-fold and 30-fold, respectively. Addition of kaempferol within 16 hours post infection (hpi) could significantly inhibit virus replication, and the DNA copies were decreased by almost 15-fold when kaempferol was added at 2 hpi. Kaempferol could regulate NF-κB and MAPKs signal pathways involved in PRV infection and change the levels of the target genes of MAPKs (ATF-2 and c-Jun) and NF-κB (IL-1α, IL-1β and IL-2) signaling pathways. All the results indicated that kaempferol has the ability to be an alternative control measure for PRV infection.¶ These authors contribute equally to this work and should be considered as the first author.
Surgery combined with antibiotics for the treatment of endogenous endophthalmitis caused by live abscess
Authors: Wang Yue , Wang Xue , Di Yu 
Published: April 2020

Abstract
BackgroundEndogenous endophthalmitis is a serious disease caused by intraocular infection, that can rapidly progress to cause blindness. This study evaluated the clinical features, surgical and antibiotics treatment strategies, and treatment outcomes in patients with endophthalmitis caused by liver abscess.MethodsBetween April 2014 and April 2019, the clinical data of patients (19 eyes) with endophthalmitis associated with liver abscess who underwent surgery at Shengjing Hospital were retrospectively analyzed. Furthermore, we also evaluated the degree of visual acuity correction in patients after surgery to determine the efficacy of vitrectomy.ResultsFifteen patients (18 eyes) underwent intravitreal injection followed by vitrectomy after admission. One patient (1 eye) was only underwent intravitreal injection. Three patients (3 eyes) had recurrent intraocular inflammation and eventually underwent evisceration. Systemic antibiotics were administered for all patients based on the results of vitreous humor culture, blood culture, and antibiotic susceptibility tests. Outpatient follow-ups were performed until the patients were stable (6 months). Of the 19 eyes, 1 eye (5.26%) had visual acuity restored to 20/200, 6 eyes (31.58%) had visual acuity restored to counting fingers (CF), 2 eyes (10.53%) had visual acuity restored to hand motion (HM), 4 eyes (21.58%) showed only light perception (LP), and the remaining 6 eyes showed no light perception (NLP),3 patients showed carbapenems resistance.ConclusionEndogenous endophthalmitis caused by liver abscess is occult, and prognosis is poor. Timely surgical intervention combined with antibiotic treatment is essential, and the primary disease must be treated to control disease progression at the earliest.
Dissecting drug and prognostic lncRNA signature mediated by DNA methylation and transcription factor in colon cancer
Authors: Zhang Jiayu , Shen Zhen , Song Zheyu , Luan Jian , Li Yezhou , Zhao Tiancheng 
Published: April 2020

Abstract
Background: Colon cancer is still the most commonly diagnosed malignancy and leading cause of death worldwide. Apart from living habits, genetic and epigenetic changes are key factors to influence the risk of colon cancer. However, the impact of epigenetic alterations in non-coding RNAs and the consequences for colon cancer has not been fully characterized.Methods: We detected differential methylation sites (DMSs) in lncRNA promoters, and identified lncQTMs by association test. To investigate TF binding affected by DNA methylation, we characterized known TF motif occurrence among DMSs collected from MEME suit. We further combined methylome and transcriptome data to construct TF-methylation-lncRNA relationships. To study the role of lncRNAs in drug response, we used pharmacological and lncRNA profiles derived from CCLE and predict drug response by lncRNA expression level. We also used the combination of TF-methylation-lncRNA relationship to stratified patient survival information by a risk model.Results: DNA methylation display global hyper-methylation character in lncRNA promoters, and they tend to have negative relationship with the corresponding lncRNAs. Negative lncQTMs located near TSS have more significant and stronger correlation with the corresponding lncRNAs. Some lncRNAs mediated by the interplay between DNA methylation and TFs are proved markers for colon cancer. Typically, lncRNA CAHM, RP11-834C11.4 and LINC00460 are good predictors for 5 drug components (17-AAG, Sorafenib, TKI258, RAF265, Topotecan) in colon cancer. And we found HES1_cg24685006_RP4-728D4.2 and SREBF1_cg05372727_LINC00460 relationships are prognostic signatures for colon cancer.Conclusions: These findings suggested lncRNAs mediated by the interplay between DNA methylation and TFs are promising predictors for drug response, besides, combined TF-methylation-lncRNA can serve as prognostic signature for colon cancer.
Non-Inferiority of Survival in Early Breast Cancer Patients with False-Negative Sentinel Nodes After Completing Axillary Lymph Node Dissection
Authors: Loh Zhu-Jun , Lee Kuo-Ting , Chen Ya-Ping , Kuo Yao-Lung , Chung Wei-Pang , Hsu Ya-Ting , Huang Chien-Chang , Hsu Hui-Ping 
Published: April 2020

Abstract
Background Sentinel lymph node biopsy (SLNB) is standard approach of axillary region for early breast cancer patients with clinically negative node. The present study investigated patients with false-negative sentinel nodes (FNSN) of intraoperative frozen section.Methods A case-control study with 1:3 ratio was conducted. FNSN was diagnosed as negative sentinel nodes (SNs) in frozen sections, but positive for metastasis in formalin-fixed paraffin-embedded (FFPE) blocks. Control was defined no metastasis of SNs in both frozen and FFPE sections.Results Total 20 FNSN cases and 60 matched-controls were enrolled from 333 SLNB patients between April 1, 2005 and November 31, 2009. The demographics and intrinsic subtypes of breast cancer were similar between FNSN and controls. The FNSN patients had a larger tumor size in preoperative mammography and more lymphatic tumor emboli in core biopsy (P-value 0.033 and < 0.001, respectively). Four FNSN patients had metastasis in the non-relevant SNs. Other 16 FNSN patients had results of benign lymphoid hyperplasia of SNs in frozen sections and metastasis in FFPE blocks. Micrometastasis (less than 2 mm) was detected in seven of 16 patients and metastases in non-sentinel nodes was recognized in two of 16 patients. All FNSN patients received second operation with axillary lymph node dissection (ALND). After a median follow-up of 143 months, no FNSN patients developed recurrence of breast cancer. The disease-free survival, disease-specific survival, and overall survival in FNSN were non-inferior than controls.Conclusion Outcomes of FNSN patients after completing ALND was non-inferior to those without metastasis in SNs. ALND improved survival of patients with metastasis non-sentinel ALNs. However, omitting ALND had no effect for those have only micrometastasis in SNs.
Anti-inflammatory and NF-κB inhibitory activity of aerial parts of Cestrum diurnum
Authors: Khatun Amina , Rahman Mahmudur , Nesa Lutfun , Looi Chung Yeng , Wong Won Fen , Hazni Hazrina , Mahdzir Mohamad Azrul bin , Uddin Shaikh Jamal , Awang Khalijah , Shilpi Jamil Ahmad 
Published: April 2020

Abstract
Background

Cestrum diurnum L. (Solanaceae) is used in various traditional medicine for pain and related disorders. The Malayali tribe of Tamil Nadu in India use the leaf in joint pain. In the Chinese traditional medicine it is used for the treatment of burns and swellings. Present study was designed to evaluate its traditional use in pain and inflammation.
Methods

Methanol extract of the aerial parts of C. diurnum was tested by carrageenan induced paw oedema and formalin induced paw licking test in mice at the oral doses of 150 and 300 mg/kg body weight. NF-κB inhibitory activity was evaluated by TNF-α induced NF-κB activation assay in RAW 264.7 macrophage cells at the concentration of 100 µg/ml.
Results

The extract, at the doses of 150 and 300 mg/kg, showed significant inhibition (p < 0.05) of carrageenan induced paw oedema and the effect persisted throughout the entire experimental period of 3 h with the highest activity (50% inhibition) at 3rd h. In formalin induced paw licking test, the extract exhibited significant (p < 0.05) inhibition of paw licking, both in the early and late phase of the experiment at the aforementioned dose levels. At the concentration of 100 µg/ml, the extract did not inhibit the nuclear translocation of NF-κB. Rather, the extract was found to downregulate NF-κB p65 protein expression.
Conclusions

The present work supports the folkloric use of the plant for its analgesic and anti-inflammatory action which might involve downregulation of NF-κB p65 protein expression and/or inhibition of autacoid (histamine, serotonin, prostaglandin) synthesis.
Detailed severity assessment of Cincinnati Prehospital Stroke Scale to detect large vessel occlusion in acute ischemic stroke
Authors: Tárkányi Gábor , Csécsei Péter , Szegedi István , Fehér Evelin , Annus Ádám , Molnár Tihamér , Szapáry László 
Published: April 2020

Abstract
Background
Selecting stroke patients with large vessel occlusion (LVO) based on prehospital stroke scales could provide a faster triage and transportation to a comprehensive stroke centre resulting a favourable outcome. We aimed here to explore the detailed severity assessment of Cincinnati Prehospital Stroke Scale (CPSS) to improve its ability to detect LVO in acute ischemic stroke (AIS) patients.
Methods
A cross-sectional analysis was performed in a prospectively collected registry of consecutive patients with first ever AIS admitted within 6 hours after symptom onset. On admission stroke severity was assessed National Institutes of Health Stroke Scale (NIHSS) and the presence of LVO was confirmed by computed tomography angiography (CTA) as an endpoint. A detailed version of CPSS (d-CPSS) was designed based on the severity assessment of CPSS items derived from NIHSS. The ability of this scale to confirm an LVO was compared to CPSS and NIHSS respectively.
Results
Using a ROC analysis, the AUC value of d-CPSS was significantly higher compared to the AUC value of CPSS itself (0.788 vs. 0.633, p < 0.001) and very similar to the AUC of NIHSS (0.795, p = 0.510). An optimal cut-off score was found as d-CPSS ≥ 5 to discriminate the presence of LVO (sensitivity: 69.9%, specificity: 75.2%).
Conclusion
A detailed severity assessment of CPSS items (upper extremity weakness, facial palsy and speech disturbance) could significantly increase the ability of CPSS to discriminate the presence of LVO in AIS patients.