bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measuring School Climate: Invariance across Middle and High School Students
Authors: Waasdorp Tracy Evian , Lindstrom Johnson Sarah , Shukla Kathan D , Bradshaw Catherine P 
Published: December 2019

Abstract
Positive school climate has been consistently associated with many desirable student outcomes in both middle and high schools. However, there has been little work comparing the perceptions across these two school settings. The U.S. Department of Education conceptualized a three-factor model for school climate consisting of safety, engagement, and environment. Drawing on data from 29,720 middle and 34,950 high school students, the fit of the three-factor model was examined for measurement invariance, to explore whether the measure functioned similarly across both middle and high schools. The results indicated measurement invariance, which suggests that practitioners and researchers can confidently compare findings across middle and high schools to inform local decision making related to school-based programming. A series of multilevel analyses also explored the extent to which perceptions of school climate differed for middle and high school students; these results generally indicated that middle school students perceived the school more favorably than high school students. Implications of these findings for social workers are considered.
Diurnal and Seasonal Activity Patterns of Drosophilid Species (Diptera: Drosophilidae) Present in Blackberry Agroecosystems With a Focus on Spotted-Wing Drosophila
Authors: Swoboda-Bhattarai Katharine A , Burrack Hannah J 
Published: December 2019

Abstract
Drosophilid species with different life histories have been shown to exhibit similar behavioral patterns related to locating and utilizing resources such as hosts, mates, and food sources. Drosophila suzukii (Matsumura) is an invasive species that differs from other frugivorous drosophilids in that females lay eggs in ripe and ripening fruits instead of overripe or rotten fruits. We hypothesized that there may be diurnal and/or seasonal patterns associated with the movement of drosophilid species into and out of crop fields and their attraction to fermentation-odor-based monitoring traps, and that D. suzukii would conform to similar patterns. To test these hypotheses, we deployed passive, 2-headed Malaise traps between crop fields and wooded edges to simultaneously catch flies moving into and out of crop fields. We also deployed monitoring traps with a fermentation-based bait between crop fields and wooded edges and within crop rows. Traps were deployed weekly in June–August in 2014 and 2015 at two commercial blackberry farm in Cleveland County, NC, and were checked hourly for 24 h, except during darkness. Both D. suzukii and other drosophilid species moved between crop fields and wooded edges and were attracted to monitoring traps primarily during the morning and evening hours. Whereas other drosophilids were captured in traps throughout the season, few D. suzukii were caught in traps until early to mid-July in both years and increased as the season progressed. Understanding D. suzukii movement and activity patterns is essential for the development of effective management strategies.
Whipple’s endocarditis: a case report of a blood culture-negative endocarditis
Authors: Scheurwater Miriam A , Verduin Cees M , van Dantzig Jan-Melle 
Published: December 2019

Abstract

Background
Whipple’s disease is caused by Tropheryma whipplei and causes a self-limiting gastrointestinal infection. The majority of the population is an asymptomatic carrier, however, in some patients, it causes an invasive infection with for example arthritis, endocarditis, or involvement of the eyes.

Case summary
This case describes a man with long-lasting complaints of progressive dyspnoea caused by heart failure due to total destruction of the aortic and mitral valve as a result of T. whipplei endocarditis, diagnosed with serum polymerase chain reaction. The patient was treated with ceftriaxone and prolonged co-trimoxazole therapy and surgical replacement of the aortic and mitral valve. He was discharged to a rehabilitation centre.

Discussion
Tropheryma whipplei is one of the possible microorganisms classified as causing blood culture-negative endocarditis, with predominantly afebrile patients that do not fulfil the Dukes criteria, which makes it difficult to diagnose. Polymerase chain reaction is the cornerstone of the diagnosis. It requires long-term antibiotic treatment up to 12 months. It is recommended by the European Society of Cardiology to discuss treatment in an Endocarditis Team because Whipple’s endocarditis has only rarely been described in the literature previously. Whipple’s endocarditis has high mortality and relapse rates.
Novel mutations in malonyl-CoA-acyl carrier protein transacylase provoke autosomal recessive optic neuropathy
Authors: Li Huiping , Yuan Shiqin , Minegishi Yuriko , Suga Akiko , Yoshitake Kazutoshi , Sheng Xunlun , Ye Jianping , Smith Stuart , Bunkoczi Gabor , Yamamoto Megumi , Iwata Takeshi 
Published: December 2019

Abstract
Inherited optic neuropathies are rare eye diseases of optic nerve dysfunction that present in various genetic forms. Previously, mutation in three genes encoding mitochondrial proteins has been implicated in autosomal recessive forms of optic atrophy that involve progressive degeneration of optic nerve and retinal ganglion cells (RGC). Using whole exome analysis, a novel double homozygous mutation p.L81R and pR212W in malonyl CoA-acyl carrier protein transacylase (MCAT), a mitochondrial protein involved in fatty acid biosynthesis, has now been identified as responsible for an autosomal recessive optic neuropathy from a Chinese consanguineous family. MCAT is expressed in RGC that are rich in mitochondria. The disease variants lead to structurally unstable MCAT protein with significantly reduced intracellular expression. RGC-specific knockdown of Mcat in mice, lead to an attenuated retinal neurofiber layer, that resembles the phenotype of optic neuropathy. These results indicated that MCAT plays an essential role in mitochondrial function and maintenance of RGC axons, while novel MCAT p.L81R and p.R212W mutations can lead to optic neuropathy.
Antipsychotic Polypharmacy: A Dirty Little Secret or a Fashion?
Authors: Lin Shih-Ku 
Published: December 2019

AbstractThe term polypharmacy was originally coined to refer to problems related to multiple drug consumption and excessive drug use during the treatment of a disease or disorder. In the treatment of schizophrenia, polypharmacy usually refers to the simultaneous use of 2 or more antipsychotic medications or combined (adjunct) medications such as mood stabilizers, antidepressants, anxiolytics, or hypnotics in addition to single or multiple antipsychotics. Two decades ago, antipsychotic polypharmacy was criticized as being more expensive, having unproven efficacy, and causing more side effects. However, in recent years, antipsychotic polypharmacy has become more or less acceptable in the views of clinical practitioners and academic researchers. Results from recent reviews have suggested that the common practice of antipsychotic polypharmacy lacks double-blind or high-quality evidence of efficacy, except for negative symptom reduction with aripiprazole augmentation. We reviewed some representative studies that enrolled large numbers of patients and compared antipsychotic polypharmacy and monotherapy during the past decade. The results revealed that a certain proportion of select patients can benefit from antipsychotic polypharmacy without further negative consequences. Because most of the current treatment guidelines from different countries and organizations prefer monotherapy and discourage all antipsychotic polypharmacy, guidelines regarding the use of antipsychotic polypharmacy in clinical practice should be revised. On the basis of the findings of 2 large-scale studies from Asia and Europe, we also suggest ideal rates of various maintenance treatments of schizophrenia, which are as follows: antipsychotic polypharmacy, 30%; combined mood stabilizer, 15%; combined antidepressant, 10%; combined anxiolytics, 30%; and combined hypnotic, 10%.
Correlation of mRNA delivery timing and protein expression in lipid-based transfection
Authors: Reiser A , Woschée D , Mehrotra N , Krzysztoń R , Strey H H , Rädler J O 
Published: December 2019

AbstractNon-viral gene delivery is constrained by the dwell time that most synthetic nucleic acid nanocarriers spend inside endosomal compartments. In order to overcome this endosomal-release bottleneck, methods are required that measure nanocarrier uptake kinetics and transfection efficiency simultaneously. Here, we employ live-cell imaging on single-cell arrays (LISCA) to study the delivery-time distribution of lipid-based mRNA complexes under varied serum conditions. By fitting a translation-maturation model to hundreds of individual eGFP reporter fluorescence time courses, the protein expression onset times and the expression rates after transfection are determined. Using this approach, we find that delivery timing and protein expression rates are not intrinsically correlated at the single-cell level, even though population-averaged values of both parameters conjointly change as a function of increasing external serum protein fraction. Lipofectamine-mediated delivery showed decreased transfection efficiency and longer delivery times with increasing serum protein concentration. This is in contrast to ionizable lipid nanoparticle (i-LNP)-mediated transfer, which showed increased efficiency and faster uptake in the presence of serum. In conclusion, the interdependences of single-cell expression rates and onset timing provide additional clues on uptake and release mechanisms, which are useful for improving nucleic acid delivery.
Phenotypic chemical and mutant screening of zebrafish larvae using an on-demand response to electric stimulation
Authors: Khalili Arezoo , Peimani Amir Reza , Safarian Nickie , Youssef Khaled , Zoidl Georg , Rezai Pouya 
Published: December 2019

Abstract
Behavioral responses of zebrafish larvae to environmental cues are important functional readouts that should be evoked on-demand and studied phenotypically in behavioral, genetical and developmental investigations. Very recently, it was shown that zebrafish larvae execute a voluntary and oriented movement toward the positive electrode of an electric field along a microchannel. Phenotypic characterization of this response was not feasible due to larva’s rapid movement along the channel. To overcome this challenge, a microfluidic device was introduced to partially immobilize the larva’s head while leaving its mid-body and tail unrestrained in a chamber to image motor behaviors in response to electric stimulation, hence achieving quantitative phenotyping of the electrically evoked movement in zebrafish larvae. The effect of electric current on the tail-beat frequency and response duration of 5–7 days postfertilization zebrafish larvae was studied. Investigations were also performed on zebrafish exposed to neurotoxin 6-hydroxydopamine and larvae carrying a pannexin1a (panx1a) gene knockout, as a proof of principle applications to demonstrate on-demand movement behavior screening in chemical and mutant assays. We demonstrated for the first time that 6-hydroxydopamine leads to electric response impairment, levodopa treatment rescues the response and panx1a is involved in the electrically evoked movement of zebrafish larvae. We envision that our technique is broadly applicable as a screening tool to quantitatively examine zebrafish larvae’s movements in response to physical and chemical stimulations in investigations of Parkinson’s and other neurodegenerative diseases, and as a tool to combine recent advances in genome engineering of model organisms to uncover the biology of electric response.
‘An indefatigable intermediary’
Authors: Rowlands Eliot W 
Published: December 2019

Abstract
At a time when museum curatorship in America was in its infancy, Harold Woodbury Parsons (1882–1967) scouted and negotiated for outstanding works of art for the cash-rich Cleveland Museum of Art, which opened to the public in 1916. As its European representative (1925–41), he acquired such masterworks as the Stroganoff Ivory, El Greco’s Holy Family with St Mary Magdalen, and the Warren tondo by Filippino Lippi, all during the late 1920s. During a lifetime’s work in the art market, in which he worked for private collectors and other museums, this was his most important achievement. What he acquired for the Cleveland Museum is vividly recounted in the art agent’s correspondence, until now, almost entirely unpublished. After moving to Rome in 1910, Parsons first served as ‘an indefatigable intermediary’ in the world market for antiquities. Later, with the blessing of Edward Waldo Forbes and Paul J. Sachs – director and assistant director, respectively, of Harvard’s Fogg Art Museum – and with a host of European contacts, he was able to ‘gun for’ art for an ever expanding number of clients.
Oral Leukoplakia and Risk of Progression to Oral Cancer: A Population-Based Cohort Study
Authors: Chaturvedi Anil K , Udaltsova Natalia , Engels Eric A , Katzel Jed A , Yanik Elizabeth L , Katki Hormuzd A , Lingen Mark W , Silverberg Michael J 
Published: December 2019

AbstractBackgroundThe optimal clinical management of oral precancer remains uncertain. We investigated the natural history of oral leukoplakia, the most common oral precancerous lesion, to estimate the relative and absolute risks of progression to cancer, the predictive accuracy of a clinician’s decision to biopsy a leukoplakia vis-à-vis progression, and histopathologic predictors of progression.MethodsWe conducted a retrospective cohort study (1996–2012) of patients with oral leukoplakia (n = 4886), identified using electronic medical records within Kaiser Permanente Northern California. Among patients with leukoplakia who received a biopsy (n = 1888), we conducted a case-cohort study to investigate histopathologic predictors of progression. Analyses included indirect standardization and unweighted or weighted Cox regression.ResultsCompared with the overall Kaiser Permanente Northern California population, oral cancer incidence was substantially elevated in oral leukoplakia patients (standardized incidence ratio = 40.8, 95% confidence interval [CI] = 34.8 to 47.6; n = 161 cancers over 22 582 person-years). Biopsied leukoplakias had a higher oral cancer risk compared with those that were not biopsied (adjusted hazard ratio = 2.38, 95% CI = 1.73 to 3.28). However, to identify a prevalent or incident oral cancer, the biopsy decision had low sensitivity (59.6%), low specificity (62.1%), and moderate positive–predictive value (5.1%). Risk of progression to oral cancer statistically significantly increased with the grade of dysplasia; 5-year competing risk-adjusted absolute risks were: leukoplakia overall = 3.3%, 95% CI = 2.7% to 3.9%; no dysplasia = 2.2%, 95% CI = 1.5% to 3.1%; mild-dysplasia = 11.9%, 95% CI = 7.1% to 18.1%; moderate-dysplasia = 8.7%, 95% CI = 3.2% to 17.9%; and severe dysplasia = 32.2%, 95% CI = 8.1%–60.0%. Yet 39.6% of cancers arose from biopsied leukoplakias without dysplasia.ConclusionsThe modest accuracy of the decision to biopsy a leukoplakia vis-à-vis presence or eventual development of oral cancer highlights the need for routine biopsy of all leukoplakias regardless of visual or clinical impression. Leukoplakia patients, particularly those with dysplasia, need to be closely monitored for signs of early cancer.
Narratives and International Reconciliation
Authors: Lind Jennifer 
Published: December 2019

AbstractIn relations between Japan and South Korea, as well as between other former adversaries, observers frequently argue that “history stands in the way” of better relations. They expect that hostile historical narratives will prevent leaders from pursuing potentially advantageous cooperation. To evaluate this claim, in this article I define narratives and their elements, noting that they range from more hostile to more friendly. I outline and theoretically develop two perspectives: the view of history as an obstacle, and a view more optimistic about the potential for cooperation and narrative transformation. Evidence from Franco-German relations after World War II, as well as other cases across time and space, supports the latter, more optimistic, view. Finally, I hypothesize different strategic and domestic conditions that make cooperation and narrative change more or less likely. Ultimately, I argue that observers have exaggerated the constraining power of narratives and thus underestimated the potential for cooperation between former enemies. This has important implications for relations between longtime rivals all over the world, and particularly in East Asia, where a conventional wisdom expects historical memories to impede balancing against China's rise.
Species turnover drives grassland community to phylogenetic clustering over long-term grazing disturbance
Authors: Zhu Juntao , Zhang Yangjian , Wang Wenfeng , Yang Xian , Chen Ning , Shen Ruonan , Wang Li , Jiang Lin 
Published: December 2019

Abstract

Aims
Grazing exerts profound effects on grassland ecosystem service and functions by regulating species composition and diversity, and structuring community assembly worldwide. However, adaptions of phylogenetic diversity and phylogenetic community structure to long-term grazing disturbance remain poorly studied, especially for ecosystems distributed in extreme environments.

Methods
Here, we conducted an experiment with multigrazing intensities to explore the impacts of grazing disturbance on plant phylogenetic diversity and community structure in an alpine grassland of the Tibetan Plateau.

Important Findings
Grazing disturbance enriched plant species richness (SR), and stimulated species turnover from regional species pool, consequently changing community species composition. Under low intensities, grazing exerted no obvious effects on phylogenetic diversity and community structure, whereas communities changed from overdispersion to clustering under high grazing intensity. High grazing intensity resulted in stronger environmental filtering, which consequently selected those species with high resilience to grazing disturbance. The observed clustering structure was associated with the colonizing species which were closely related to resident species, and locally extinct species, and distantly related to residents. At the plant functional trait level, high grazing intensity increased species colonization largely by altering the effect of root depth on species colonization compared to light grazing. Our results highlight that solely utilization of SR and diversity cannot fully represent grassland communities responses to grazing. The effects of species turnover on community phylogenetic diversity and structure are entailed to be explored in the future grazing studies.
Natural selection on flower size in invasive Cytisus scoparius along an elevation gradient
Authors: Bode Robert Frederick , Dufresne Catherine 
Published: December 2019

AbstractAimsEstablished invasive plant species have more diverse gene pools than single populations in their home range. This genetic and often phenotypic variation allows for natural selection to act and produce rapid adaptations as an invasive species interacts with other members of the community. For an invasive flowering plant, interactions with pollinators may lead to adaptations to a heterogeneous pollinator community. The variation in the pollinator community in the invaded range is similar to variation in the native range, suggesting that adaptations may mirror patterns seen in the home range. In this study, we investigated variation in flower size in Cytisus scoparius, an invasive leguminous shrub, along an elevation gradient.MethodsThis study took place in the Capitol State Forest in Washington State. We measured flower widths and the proportion of visited flowers, using 10 plants per field site. Sites were located along an elevation gradient and were visited three times to measure phenological variation on a per-plant basis.Important FindingsWe saw positive selection for flower size, albeit without a distinct pattern of higher selection at higher elevations. The pattern of natural selection could be seen both in terms of pollinator visitation rates and in seed production. We also found that the largest contributor to changes in seed production was not elevation or flower size but management practices.
Enhancement of membrane lipid peroxidation in lung cancer cells irradiated with monoenergetic X-rays at the K-shell resonance absorption peak of phosphorus
Authors: Maezawa Hiroshi , Indo Hiroko P , Usami Noriko , Majima Hideyuki J , Ito Hiromu , Ohnishi Ken , Kobayashi Katsumi 
Published: December 2019

AbstractThe aim of this study was to determine whether membrane lipid peroxidation in mammalian cells is enhanced by X-ray irradiation at the K-shell resonance absorption peak of phosphorus. A549 and wild-type p53-transfected H1299 (H1299/wtp53) cell lines derived from human lung carcinoma were irradiated with monoenergetic X-rays at 2.153 keV, the phosphorus K-shell resonance absorption peak, or those at 2.147 or 2.160 keV, which are off peaks. Immunofluorescence staining for 4-hydroxy-2-nonenal (HNE), a lipid peroxidation product, was used as marker for protein modification. In both cell lines, the HNE production was significantly enhanced after irradiation at 2.153 keV compared to sham-irradiation. The enhancement (E) was calculated as the ratio of the fluorescence intensity of irradiated cells to that of sham-irradiated cells. In both the cell lines, E2.153 was significantly larger than E2.147 and no significant difference between E2.147 and E2.160 was observed. The extra enhancement at 2.153 keV was possibly caused by energy transition within the phosphorus K-shell resonance absorption. Our results indicate that membrane lipid peroxidation in cells is enhanced by the Auger effect after irradiation at the K-shell resonance absorption peak of phosphorus rather than by the photoelectric effect of the constituent atoms in the membrane lipid at 2.147 keV.
Living in Permanent Temporariness: The Multigenerational Ordeal of Living under Germany’s Toleration Status
Authors: Tize Carola 
Published: December 2019

AbstractToleration status (temporary suspension of deportation) has been Germany’s hesitant answer to providing humanitarian relief for Palestinians escaping the dismal conditions of refugee camps and the civil war in Lebanon in the late 1980s and early 1990s. At the same time, the status has subjected families to years, even decades, of insecurity and uncertainty through constant threats of deportation and restrictions on work, travel and higher education. Based on 19 months of ethnographic research, the article shows the story of one family during their 16 years on toleration status and their experiences after gaining permanent residency. The family’s experiences illuminate the insecurity and uncertainty large communities on toleration status in Berlin-Neukölln experienced, all sharing the fate of constantly wavering between hope, fear and disillusionment. Their struggles also show how the permanent temporariness of long-term toleration status affects both the parents who fled the conflict and their children, most of whom were born and raised in Germany. I argue that toleration status limits the capabilities of children stuck in the stagnant realities of their family’s insecure status, along the lines of gender and birth order. Moreover, toleration status as a multigenerational ordeal persists long after the legal insecurity has ended.
An intrathoracic mass behind severe immunodeficiency: a case report of Good’s syndrome and large type AB thymoma
Authors: Thuraisingam Amalan 
Published: December 2019

Abstract
A large solitary intrathoracic mass was identified in a patient with concurrent immunodeficiency. The patient was found to have a type AB thymoma and was diagnosed with Good’s syndrome. A case report demonstrates the importance of timely surgical intervention for this rare syndrome.