bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of doxorubicin‐induced cardiotoxicity on cardiac mitochondrial dynamics and mitochondrial function: Insights for future interventions
Authors: Osataphan Nichanan , Phrommintikul Arintaya , Chattipakorn Siriporn C. , Chattipakorn Nipon 
Published: April 2020

AbstractAnthracyclines is an effective chemotherapeutic treatment used for many types of cancer. However, high cumulative dosage of anthracyclines leads to cardiac toxicity and heart failure. Dysregulation of mitochondrial dynamics and function are major pathways driving this toxicity. Several pharmacological and non‐pharmacological interventions aiming to attenuate cardiac toxicity by targeting mitochondrial dynamics and function have shown beneficial effects in cell and animal models. However, in clinical practice, there is currently no standard therapy for the prevention of anthracycline‐induced cardiotoxicity. This review summarizes current reports on the impact of anthracyclines on cardiac mitochondrial dynamics and mitochondrial function and potential interventions targeting these pathways. The roles of mitochondrial dynamics and mitochondrial function in the development of anthracycline‐induced cardiotoxicity should provide insights in devising novel strategies to attenuate the cardiac toxicity induced by anthracyclines.
Lysophosphatidic acid improves corneal endothelial density in tissue culture by stimulating stromal secretion of interleukin‐1β
Authors: Hsueh Yi‐Jen , Meir Yaa‐Jyuhn James , Lai Jui‐Yang , Chen Hung‐Chi , Ma David Hui‐Kang , Huang Chieh‐Cheng , Lu Tsai‐Te , Cheng Chao‐Min , Wu Wei‐Chi 
Published: April 2020

AbstractThe short supply of donor corneas is exacerbated by the unsuitability of donors with insufficient endothelial cell density. Few studies have investigated promoting corneal endothelial cell proliferation to increase the endothelial cell density. We hypothesize that pre‐transplantation treatment of proliferative tissue‐cultivated corneas may increase corneal endothelial cell density. We observed that the airlift cultures were superior to immersion cultures with respect to both transparency and thickness. In this tissue culture system, we observed that lysophosphatidic acid increased the rabbit corneal endothelial cell density, number of BrdU‐positive cells and improve wound healing. We also observed an indirect effect of lysophosphatidic acid on corneal endothelial cell proliferation mediated by the stimulation of interleukin‐1β secretion from stromal cells. Human corneal tissues treated with lysophosphatidic acid or interleukin‐1β contained significantly more Ki‐67‐positive cells than untreated group. The lysophosphatidic acid‐ or interleukin‐1β‐treated cultured tissue remained hexagon‐shaped, with ZO‐1 expression and no evidence of the endothelial‐mesenchymal transition. Our novel protocol of tissue culture may be applicable for eye banks to optimize corneal grafting.
Longitudinal patterns of cytokine expression at the individual level in humans after laparoscopic sleeve gastrectomy
Authors: Trahtemberg Uriel , Darawshe Fares , Elazary Ram , Ginsburg Isaac , Beil Michael , van Heerden Peter Vernon , Sviri Sigal 
Published: April 2020

AbstractThe study of the human response to injury has been hampered by the inherent heterogeneity in the models and methods used. By studying a standard injury longitudinally, using individual patient‐level analysis, we endeavoured to better describe its dynamics. We analysed clinical variables, clinical laboratory and plasma cytokines from 20 patients at five time points. Clustering analysis showed two prototype patterns of cytokine behaviour: a concordant type, where cytokines behave the same way for all patients (notably IL‐0 and TNFα), and a variable type, where different patterns of expression are seen for different patients (notably IL‐8, IL‐6 and IL‐1RA). Analysis of the cytokines at the individual patient‐level showed a strong four‐way correlation between IL‐1RA, GCSF, MIP‐1β and MCP‐1. As it holds for most patients and not just on average, this suggests that they form a network which may play a central role in the response to gastro‐intestinal injuries in humans. In conclusion, the longitudinal analysis of cytokines in a standard model allowed the identification of their underlying patterns of expression. We propose that the two prototype patterns shown may reflect the mechanism that separates the common and individual aspects of the injury response.
Condition‐dependent mortality exacerbates male (but not female) reproductive senescence and the potential for sexual conflict
Authors: Sultanova Zahida , García‐Roa Roberto , Carazo Pau 
Published: April 2020

AbstractDisentangling the relationship between age and reproduction is central to understand life‐history evolution, and recent evidence shows that considering condition‐dependent mortality is a crucial piece of this puzzle. For example, nonrandom mortality of ‘low‐condition’ individuals can lead to an increase in average lifespan. However, selective disappearance of such low‐condition individuals may also affect reproductive senescence at the population level due to trade‐offs between physiological functions related to survival/lifespan and the maintenance of reproductive functions. Here, we address the idea that condition‐dependent extrinsic mortality (i.e. simulated predation) may increase the age‐related decline in male reproductive success and with it the potential for sexual conflict, by comparing reproductive ageing inDrosophila melanogastermale/female cohorts exposed (or not) to condition‐dependent simulated predation across time. Although female reproductive senescence was not affected by predation, male reproductive senescence was considerably higher under predation, due mainly to an accelerated decline in offspring viability of ‘surviving’ males with age. This sex‐specific effect suggests that condition‐dependent extrinsic mortality can exacerbate survival‐reproduction trade‐offs in males, which are typically under stronger condition‐dependent selection than females. Interestingly, condition‐dependent extrinsic mortality did not affect mating success, hinting that accelerated reproductive senescence is due to a decrease in male post‐copulatory fitness components. Our results support the recent proposal that male ageing can be an important source of sexual conflict, further suggesting this effect could be exacerbated under more natural conditions.
Dietary patterns are associated with likelihood of hepatic steatosis among US adults
Authors: Mazidi Mohsen , Ofori‐Asenso Richard , Kengne Andre Pascal 
Published: April 2020

AbstractBackground and AimNon‐alcoholic fatty liver disease is a rapidly growing public health problem. In this study, we explored the association between dietary patterns (DPs) and fatty liver and liver function tests.MethodsThis was a cross‐sectional study using data from the US community‐based National Health and Nutrition Examination Survey. Participants with data on dietary intake, blood pressure, and status for diabetes mellitus were analyzed. DPs were determined by principal components analysis. Analysis of covariance and logistic regression models accounted for the survey design and sample weights.ResultsOf the 20 643 eligible participants, 45.7% had prevalent fatty liver. Three DPs collectively explained 50.8% of variance in dietary nutrients consumption. The first DP was representative of a diet containing high levels of saturated and mono‐unsaturated fatty acids, total fat and carbohydrate; the second DP comprised vitamins, minerals and dietary fibre; and the third DP was mainly representative of polyunsaturated fatty acids. In adjusted multivariable regression models, participants in the top quarter of the second DP had 34% lower odds of prevalent fatty liver (odds ratio 0.66 [95% confidence interval [CI]: 0.43–0.71]), while those in the top quarter of the first DP had 86% higher odds (1.86 [95% CI: 1.42–2.95]) of prevalent fatty liver, relative to participants in the bottom quarter of each of the DPs.ConclusionOur findings suggest that a diet with high load of vitamins, minerals, and fiber content is associated with a lower prevalence of non‐alcoholic fatty liver disease.
Obesity and regulation of human placental lactogen production in pregnancy
Authors: Cattini Peter A. , Jin Yan , Jarmasz Jessica S. , Noorjahan Noshin , Bock Margaret E. 
Published: April 2020

AbstractThe four genes coding for placental members of the human (h) growth hormone (GH) family include two that code independently for placental lactogen (PL), also known as chorionic somatomammotrophin hormone, one that codes for placental growth hormone (PGH) and a pseudogene for which RNA but no protein product is reported. These genes are expressed preferentially in the villus syncytiotrophoblast of the placenta in pregnancy. In higher primates, the placental members, including hPL and PGH, are the result of multiple duplication events of the GH gene. This contrasts with rodents and ruminants, where PLs result from duplication of the prolactin (PRL) gene. Thus, unlike their mouse counterparts, the hPL and PGH hormones bind both lactogenic and somatogenic receptors with varying affinity. Roles influenced by nutrient availability in both metabolic control in pregnancy and maternal behaviour are supported. However, the effect maternal obesity has on the activation of placental members of the hGH gene family, particularly the expression and function of those genes, is poorly understood. Evidence from partially humanised hGH/PL transgenic mice indicates that both the remote upstream hPL locus control region (LCR) and more gene‐related regulatory regions are required for placental expression in vivo. Furthermore, a specific pattern of interactions between the LCR and hPL gene promoter regions is detected in term placenta chromatin from women with a normal body mass index (BMI) in the range 18.5–25 kg m‐2 by chromosome conformation capture assay. This pattern is disrupted with maternal obesity (class II BMI > 35 kg m‐2) and associated with a > 40% decrease in term hPL RNA levels, as well as serum hPL but not PRL levels, during pregnancy. The relative importance of the chromosomal architecture and predicted properties for transcription factor participation in terms of hPL production and response to obesity are considered, based on comparison with components required for efficient human pituitary GH gene expression.
Variation of biophysical parameters of the skin with age, gender, and lifestyles
Authors: Zhao Chaoshuai , Wang Xin , Mao Yaqi , Xu Ziqian , Sun Yue , Mei Xingyu , Shi Weimin 
Published: April 2020

AbstractBackgroundSweet, spicy or greasy food, staying up late, and using electronic products for a long time are common bad habits nowadays. Their role in skin diseases has been paid much attention.ObjectiveThe aim of this study was to investigate whether unhealthy lifestyles would affect the skin sebum content, SC hydration, and pH and how do they affect.MethodsA total of 300 volunteers were enrolled, and a multifunctional skin physiology monitor measured the three skin biophysical properties on the forehead and dorsal hand. Lifestyle factors were evaluated by a self‐administered questionnaire.ResultsEating oily, sweet, spicy food, and staying up late increased the sebum content of the forehead significantly. Dorsal hand SC hydration was higher in people eating more sweet food and oily food, and forehead SC hydration was higher in people eating more sweet food and go to bed earlier. Eating sweet food could increase pH in both forehead and dorsal hand. The forehead pH decreased in using electronic products over 6 hours a day or staying up late. There are significant differences in sebum, hydration, and pH value among different age groups. In males, the pH was lower than females, but the sebum was higher.ConclusionSebum content, SC hydration, and pH are affected by unhealthy lifestyles, age, and gender.
Low‐Risk Anomalies?
Authors: SCHNEIDER PAUL , WAGNER CHRISTIAN , ZECHNER JOSEF 
Published: April 2020

ABSTRACTThis paper shows that low‐risk anomalies in the capital asset pricing model and in traditional factor models arise when investors require compensation for coskewness risk. Empirically, we find that option‐implied ex ante skewness is strongly related to ex post residual coskewness, which allows us to construct coskewness factor‐mimicking portfolios. Controlling for skewness renders the alphas of betting‐against‐beta and betting‐against‐volatility insignificant. We also show that the returns of beta‐ and volatility‐sorted portfolios are driven largely by a single principal component, which in turn is explained largely by skewness.
Occurrence and pathogenicity of Enterobacter sp. causing sprout decay and seedling stunting of upland cotton (Gossypium hirsutum L.)
Authors: Nagrale Dipak T. , Gawande Shailesh P. , Hiremani Neelakanth S. , Gokte‐Narkhedkar Nandini 
Published: April 2020

AbstractA new sprout decay and seedling stunting disease of unknown aetiology in upland cotton (Gossypium hirsutum L.) affecting nearly 5%–10% of young seedlings was noticed in vertisols of central Vidarbha (Maharashtra state, India) in July of 2017. The bacterium was consistently isolated from diseased seedlings and identified with a polyphasic method of characterization, including morphological, physiological, biochemical and 16S rRNA gene sequence analysis. The bacterium strain CICR‐MGMG1 was isolated from diseased plants identified as Enterobacter sp. Inoculation of healthy cotton seed with an axenic culture of strain CICR‐MGMG1 isolated from diseased young seedling reproduced disease symptoms of yellowing, stunting and deformed growth similar to the symptoms reported from infected field condition. The strain CICR‐MGMG1 was consistently isolated from both diseased seedlings and stunted plants. Thus, the pathogenicity test of Koch's postulates was confirmed with the bacterium Enterobacter sp. strain CICR‐MGMG1 as the causal organism of sprout decay and seedling stunting. To the best of our knowledge, this is the first record of Enterobacter sp. causing sprouts decay and seedling stunting of cotton.
Weaning affects the glycosidase activity towards phenolic glycosides in the gut of piglets
Authors: Van Noten Noémie , Van Liefferinge Elout , Degroote Jeroen , De Smet Stefaan , Desmet Tom , Michiels Joris 
Published: April 2020

AbstractPhenolic compounds in pig diets, originating either from feed ingredients or additives, may occur as glycosides, that is conjugated to sugar moieties. Upon ingestion, their bioavailability and functionality depend on hydrolysis of the glycosidic bond by endogenous or microbial glycosidases. Hence, it is essential to map the glycosidase activities towards phenolic glycosides present along gut. Therefore, the activity of three key glycosidases, that is α‐glucosidase (αGLU), β‐glucosidase (βGLU) and β‐galactosidase (βGAL), was quantified in small intestinal mucosa and digesta of piglets at different gastrointestinal sites (stomach, three parts of small intestine, caecum and colon) and at different ages around weaning (10 days before and 0, 2, 5, 14 and 28 days after weaning). Activity assays were performed with p‐nitrophenyl glycosides at neutral pH. The αGLU activities in mucosa and digesta were low (overall means 1.4 and 60 U respectively) as compared to βGLU (15.2 and 199 U) and βGAL (23.4 and 298 U; p < .001). Moreover, αGLU activity in mucosa was unaffected by age. Conversely, βGLU and βGAL activities dropped significantly after weaning. Minimal levels, ranging between 18% and 54% of the pre‐weaning values, were reached at 5 days post‐weaning. Similarly, in small intestinal digesta, reductions from 60% up to 90% were observed for the three enzyme activities on day five post‐weaning as compared to pre‐weaning levels. In caecal contents, activities were lowest at 14 days post‐weaning, while in stomach and colon no clear weaning‐induced effects were observed. Our data suggest that weaning affects the glycosidase activity in mucosa (mainly endogenous origin) and digesta (primarily bacterial origin) with the most pronounced effects occurring 5 days post‐weaning. Moreover, differences in activities exist between different glycosidases and between gut locations. These insights can facilitate the prediction of the fate of existing and newly synthetized glycosides after oral ingestion in piglets.
Cinnamic acid decreases periodontal inflammation and alveolar bone loss in experimental periodontitis
Authors: Karatas Ozkan , Balci Yuce Hatice , Taskan Mehmet Murat , Gevrek Fikret , Alkan Cemil , Isiker Kara Gozde , Temiz Cengiz 
Published: April 2020

AbstractBackground and ObjectivePeriodontitis is the chronic destructive disease of the periodontium, which causes severe inflammation in the tissues. Cinnamic acid as an unsaturated carboxylic acid might prevent inflammation and periodontal destruction. The present study aimed to evaluate the effects of cinnamic acid in two different forms as free cinnamic acid and cinnamic acid liposome on experimental periodontitis in Wistar rats.MethodsThirty‐two female rats were used in the present study. Four main groups were created as follows: C: control group; P: periodontitis group; C‐P: free cinnamic acid‐administered periodontitis group; and CL‐P: cinnamic acid liposome applied group. Periodontitis was induced via ligating 4‐0 silk sutures around lower first molar teeth on both right and left mandibles. The study duration was 30 days, and the ligatures were removed from half of the rats in the periodontitis‐induced groups. The other half carried the ligatures throughout 30 days, and all rats were euthanized at 30th day. Mandibles were removed and evaluated via stereomicroscope and underwent histological procedures. Inflammatory cell counts, osteoblast, and osteoclast cell counts were determined in hematoxylin‐eosin–stained slides, and peroxisome proliferator–activated receptor (PPAR)‐γ, cyclooxygenase (COX)‐2, receptor activator of nuclear factor κ‐B (RANKL), and osteoprotegerin (OPG) expressions were evaluated by immunohistochemistry.ResultsControl group had the lowest bone loss, and periodontitis group which kept ligatures had the highest bone loss compared to the other groups. Ligature removal provided significant improvement in bone measurements. Cinnamic acid groups also showed lower bone loss compared to the periodontitis group. The inflammatory cell and osteoclast counts were also higher in the periodontitis group, and both applications of cinnamic acid decreased these values. Osteoblast cells were the lowest in the periodontitis group, and cinnamic acid increased these counts. PPAR‐γ and COX‐2 levels were higher in the periodontitis group, and cinnamic acid decreased these levels but not to a significant level except for the cinnamic acid liposome ligature removal group, which had significantly lower values in the PPAR‐γ and COX‐2. OPG levels were lower in the periodontitis group compared to the other groups. Cinnamic acid significantly decreased RANKL and increased OPG levels.ConclusionPeriodontitis caused increased inflammation and bone destruction accompanied by increased PPAR‐γ, COX‐2, and RANKL levels and osteoclast counts. Cinnamic acid decreased osteoclast counts and inflammation and increased osteoblast counts and OPG expression in the present animal model of periodontitis.
Novel hepatitis B virus surface antigen mutations associated with occult genotype B hepatitis B virus infection affect HBsAg detection
Authors: Wang Hao , Wang Min , Huang Jieting , Xu Ru , Liao Qiao , Shan Zhengang , Zheng Yourong , Rong Xia , Tang Xi , Li Tingting , Wang Wenjing , Li Chengyao , Fu Yongshui 
Published: April 2020

AbstractThe causative factors of occult hepatitis B infection are complicated and not yet been fully elucidated. Mutations in hepatitis B virus (HBV) S gene are one of the factors may contributing to occult infection. In this study, 89 blood donors with genotype B occult HBV infection were investigated. Fifty‐seven hepatitis B surface antigen (HBsAg)‐positive/HBV DNA‐positive blood donors served as control group for comparison. Occult HBV‐related mutations with a high incidence (P < .05) in the S gene were identified. To further verify these occult infection‐related mutations, a conservative full‐gene expression vector of HBV B genotype (pHBV1.3B) was constructed. Then, the mutant plasmids on the basis of pHBV1.3B were constructed and transfected into HepG2 cells. Extracellular as well as intracellular HBsAg was analysed by electrochemical luminescence and cellular immunohistochemistry. Ten occult infection‐related mutations (E2G, Q101R, K122R, M133T, D144E, G145R, V168A, S174N, L175S and I226S) were significantly more frequent in the occult infection group (P < .05). Five of the ten mutations (E2G, D144E, G145R, V168A and S174N) strongly decreased extracellular HBsAg level (P < .05) in the transfection system. Notably, the E2G mutation had the most significant impact on the ratio of extracellular HBsAg (3.8% vs pHBV1.3B) and intracellular HBsAg (239.3% vs pHBV1.3B) (P < .05), and the fluorescence density of E2G mutant HBsAg was significantly higher than that of pHBV1.3B (P < .0001). Hence, ten mutations were associated with genotype B occult HBV infection; E2G and V168A were novel mutations which we confirmed significantly affect HBsAg detection. E2G might cause HBsAg secretion impairment that results in intracellular accumulation and a decrease in HBsAg secretion.
Genomic data reveal international lineages of critical priority Escherichia coli harbouring wide resistome in Andean condors (Vultur gryphus Linnaeus, 1758)
Authors: Fuentes‐Castillo Danny , Esposito Fernanda , Cardoso Brenda , Dalazen Gislaine , Moura Quézia , Fuga Bruna , Fontana Herrison , Cerdeira Louise , Dropa Milena , Rottmann Jürgen , González‐Acuña Daniel , Catão‐Dias José L. , Lincopan Nilton 
Published: April 2020

AbstractCritical priority pathogens have globally disseminated beyond clinical settings, thereby threatening wildlife. Andean Condors (Vultur gryphus) are essential for ecosystem health and functioning, but their populations are globally near threatened and declining due to anthropogenic activities. During a microbiological and genomic surveillance study of critical priority antibiotic‐resistant pathogens, we identified pandemic lineages of multidrug‐resistant extended‐spectrum β‐lactamase (ESBL)‐producing Escherichia coli colonizing Andean Condors admitted at two wildlife rehabilitation centres in South America. Genomic analysis revealed the presence of genes encoding resistance to hospital and healthcare agents among international E. coli clones belonging to sequence types (STs) ST162, ST602, ST1196 and ST1485. In this regard, the resistome included genes conferring resistance to clinically important cephalosporins (i.e., CTX‐M‐14, CTX‐M‐55 and CTX‐M‐65 ESBL genes), heavy metals (arsenic, mercury, lead, cadmium, copper, silver), pesticides (glyphosate) and domestic/hospital disinfectants, suggesting a link with anthropogenic environmental pollution. On the other hand, the presence of virulence factors, including the astA gene associated with outbreak of childhood diarrhoea and extra‐intestinal disease in animals, was identified, whereas virulent behaviour was confirmed using the Galleria mellonella infection model. E. coli ST162, ST602, ST1196 and ST1485 have been previously identified in humans and food‐producing animals worldwide, indicating that a wide resistome could contribute to rapid adaptation and dissemination of these clones at the human–animal–environment interface. Therefore, these results highlight that Andean Condors have been colonized by critical priority pathogens, becoming potential environmental reservoirs and/or vectors for dissemination of virulent and antimicrobial‐resistant bacteria and/or their genes, in associated ecosystems and wildlife.