bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acetyl-11-keto-β-boswellic acid enhances the cisplatin sensitivity of non-small cell lung cancer cells through cell cycle arrest, apoptosis induction, and autophagy suppression via p21-dependent signaling pathway
Authors: Lv Minghe , Zhuang Xibing , Zhang Qi , Cheng Yunfeng , Wu Duojiao , Wang Xiangdong , Qiao Tiankui 
Published: June 2020

AbstractCisplatin-based therapy is a widely used chemotherapeutic regimen for non-small cell lung cancer (NSCLC); however, drug resistance limits its efficacy. Acetyl-11-keto-β-boswellic acid (AKBA), a bioactive compound from frankincense, has been shown to exert anti-cancer effects. The aim of this study is to explore the potential of AKBA in combination with cisplatin as a new regimen for NSCLC. CCK8 assay and clone formation assay were used to determine the effects of AKBA in combination with cisplatin on cell viability of NSCLC cell lines. A three-dimensional spherification assay was used to simulate in vivo tumor formation. Flow cytometry was performed to examine cell cycle distribution and the percentages of apoptotic cells. The associated proteins and mRNA of cell cycle, apoptosis, and autophagy were measured by western blotting and real-time fluorescence quantitative PCR. Immunofluorescence assay was used to test apoptotic nuclei and autolysosome. Small interfering RNA experiments were used to silence the expression of p21. Combination treatment of AKBA and cisplatin inhibited cell viability, clone formation, and three-dimensional spherification, enhanced G0/G1 phase arrest, increased the percentages of apoptotic cells, and decreased the ratio of positive autolysosomes, compared with cisplatin alone. AKBA in combination with cisplatin suppressed the protein expressions of cyclin A2, cyclin E1, p-cdc2, CDK4, Bcl-xl, Atg5, and LC3A/B, and upregulated p27 and p21 mRNA levels in A549 cells. Downregulation of p21 decreased G0/G1 phase arrest and the percentages of apoptotic cells, and promoted autophagy in NSCLC A549 cells. Our study demonstrates that AKBA enhances the cisplatin sensitivity of NSCLC cells and that the mechanisms involve G0/G1 phase arrest, apoptosis induction, and autophagy suppression via targeting p21-dependent signaling pathway.
Methylsulfonylmethane sensitizes endometrial cancer cells to doxorubicin
Authors: Kowalska Karolina , Habrowska-Górczyńska Dominika Ewa , Kurczewska Dominika , Domińska Kamila , Urbanek Kinga Anna , Piastowska-Ciesielska Agnieszka Wanda 
Published: June 2020

Abstract
Background
Methylsulfonylmethane (MSM) is a commonly used diet supplement believed to decrease the inflammation in joints and fastens recovery in osteoarthritis, gastric mucosal injury, or obesity-related disorders. It was also suggested that MSM might play a beneficial role in cancer treatment.

Purpose
So far, the MSM might have a potentially beneficial effect in endometrial cancer (EC) treatment.

Study design
This study evaluated the effect and usefulness of MSM in combinatory therapy with known drug doxorubicin (DOX).

Methods
The effect of combinational treatment of MSM and DOX on the induction of apoptosis was evaluated in EC cell lines (ISHIKAWA, MFE-296, MFE-280).

Results
We observed that MSM itself induces apoptosis in EC cell lines, and pre-treatment with MSM for 24 h increases the sensitivity of EC cells to DOX-induced apoptosis and DNA damage and that effect might be regulated by p42/44 (Erk1/2) MAPK and Akt (protein kinase B).

Conclusion
These results for the first time show that MSM might act as a sensitizer of EC cells to known drugs, for which EC cells quickly acquire resistance.
Tailored covalently cross-linked hydrogels based on oxidized cellulose sulfate and carboxymethyl chitosan by targeted adjustment of the storage modulus
Authors: Strätz Juliane , Fischer Steffen 
Published: June 2020

AbstractIn general hydrogel synthesis uses a trial and error method when creating new hydrogels and searches afterwards for a fitting application of the received hydrogel. A much more effective and sustainable approach would be to have an application in mind before starting a targeted synthesis of a hydrogel with specific properties. Hence the goal of the study is to investigate influencing factors—crosslink density here represented through the degree of substitution, mass average molecular mass, mixing ratio and time for gelation—to the storage modulus $$G'$$

G
′

for covalently cross-linked hydrogels based on oxidized cellulose sulfate and carboxymethyl chitosan. The results can be summarized as follows: higher values for $$G'$$

G
′

can be reached by increasing the mass average molecular mass, the crosslink density and by shifting the mixing ratio toward the polymer which determines the number of possible cross-linkings in the hydrogel. The findings of this work may be transferred to similar systems and are helpful for a targeted synthesis of hydrogels with specific characteristics.
The ‘Exposed’ Population, Violent Crime in Public Space and the Night-time Economy in Manchester, UK
Authors: Haleem Muhammad Salman , Do Lee Won , Ellison Mark , Bannister Jon 
Published: June 2020

AbstractThe daily rhythms of the city, the ebb and flow of people undertaking routines activities, inform the spatial and temporal patterning of crime. Being able to capture citizen mobility and delineate a crime-specific population denominator is a vital prerequisite of the endeavour to both explain and address crime. This paper introduces the concept of an exposed population-at-risk, defined as the mix of residents and non-residents who may play an active role as an offender, victim or guardian in a specific crime type, present in a spatial unit at a given time. This definition is deployed to determine the exposed population-at-risk for violent crime, associated with the night-time economy, in public spaces. Through integrating census data with mobile phone data and utilising fine-grained temporal and spatial violent crime data, the paper demonstrates the value of deploying an exposed (over an ambient) population-at-risk denominator to determine violent crime in public space hotspots on Saturday nights in Greater Manchester (UK). In doing so, the paper illuminates that as violent crime in public space rises, over the course of a Saturday evening, the exposed population-at-risk falls, implying a shifting propensity of the exposed population-at-risk to perform active roles as offenders, victims and/or guardians. The paper concludes with a discussion of the theoretical and policy relevance of these findings.
Regulators and villains: the dual role of private actors in diamonds and caviar
Authors: Siegel Dina , Spapens Toine , van Uhm Daan 
Published: June 2020

AbstractIn the past decades, private actors have become key actors in regulation and enforcement of various forms of trade. In this paper, we focus on the role of private actors in the regulation of the trade in diamonds and caviar. We examine the stages of calling for additional regulation; setting regulatory requirements; and determining and effectuating compliance mechanisms. Our analysis illustrates that private actors may play important roles in this process, but at the same time be unaware of loopholes that illegal operators may exploit, whereas others may commit crimes themselves. These dual roles reflect the various activities of private actors in different representations. We argue in this article that the increased involvement of private actors in processes of regulation may not only benefit but also constrain the effectivity, competence and meaning of such regulatory frameworks within contemporary society.
Comparing the effect of positioning on cerebral autoregulation during radical prostatectomy: a prospective observational study
Authors: Beck Stefanie , Ragab Haissam , Hoop Dennis , Meßner-Schmitt Aurélie , Rademacher Cornelius , Kahl Ursula , von Breunig Franziska , Haese Alexander , Graefen Markus , Zöllner Christian , Fischer Marlene 
Published: June 2020

Abstract
Purpose
Surgery in the prolonged extreme Trendelenburg position may lead to elevated intracranial pressure and compromise cerebral hemodynamic regulation. We hypothesized that robot-assisted radical prostatectomy with head-down tilt causes impairment of cerebral autoregulation compared with open retropubic radical prostatectomy in the supine position.

Methods
Patients scheduled for elective radical prostatectomy were included at a tertiary care prostate cancer clinic. Continuous monitoring of the cerebral autoregulation was performed using the correlation method. Based on measurements of cerebral oxygenation with near-infrared spectroscopy and invasive mean arterial blood pressure (MAP), a moving correlation coefficient was calculated to obtain the cerebral oxygenation index as an indicator of cerebral autoregulation. Cerebral autoregulation was measured continuously from induction until recovery from anesthesia.

Results
There was no significant difference in cerebral autoregulation between robot-assisted and open retropubic radical prostatectomy during induction (p = 0.089), intraoperatively (p = 0.162), and during recovery from anesthesia (p = 0.620). Age (B = 0.311 [95% CI 0.039; 0.583], p = 0.025) and a higher difference between baseline MAP and intraoperative MAP (B = 0.200 [95% CI 0.073; 0.327], p = 0.002) were associated with impaired cerebral autoregulation, whereas surgical technique was not (B = 3.339 [95% CI  1.275; 7.952], p = 0.155).

Conclusion
Compared with open radical prostatectomy in the supine position, robot-assisted surgery in the extreme Trendelenburg position with capnoperitoneum did not lead to an impairment of cerebral autoregulation during the perioperative period in our study population.
Trial registration number: DRKS00010014, date of registration: 21.03.2016, retrospectively registered.
Upper and Lower Bounds for the Synchronizer Performance in Systems with Probabilistic Message Loss
Authors: Zeiner Martin , Schmid Ulrich 
Published: June 2020

AbstractIn this paper, we revisit the performance of theα-synchronizer in distributed systems with probabilistic message loss as introduced in Függer et al. [Perf. Eval. 93(2015)]. In sharp contrast to the infinite-state Markov chain resp. the exponential-size finite-state upper bound presented in the original paper, we introduce a polynomial-size finite-state Markov chain for a new synchronizer variant$\alpha ^{\prime }$α′, which provides a new upper bound on the performance of theα-synchronizer. Both analytic and simulation results show that our new upper bound is strictly better than the existing one. Moreover, we show that a modified version of the$\alpha ^{\prime }$α′-synchronizer provides a lower bound on the performance of theα-synchronizer. By means of elaborate simulation results, we show that our new lower bound is also strictly better than the lower bound presented in the original paper.
Field surveys and numerical modeling of the August 2016 Typhoon Lionrock along the northeastern coast of Japan: the first typhoon making landfall in Tohoku region
Authors: Heidarzadeh Mohammad , Iwamoto Takumu , Takagawa Tomohiro , Takagi Hiroshi 
Published: June 2020

AbstractTyphoon Lionrock, also known as the national number 1610 in Japan, caused severe flooding in east Japan in August 28–31, 2016, leaving a death toll of 22. With a maximum sustained wind speed of ~ 220 km/h from the Joint Typhoon Warning Center’s best track, Lionrock was classified as a category 4 hurricane in Saffir–Simpson Hurricane Wind Scale and as a typhoon in Japan Meteorological Agency’s scale. Lionrock was among unique typhoons as it started its landfall from north of Japan. Here, we studied the characteristics of this typhoon through tide gauge data analysis, field surveys and numerical modeling. Tide gauge analysis showed that the surges generated by Lionrock were in the ranges of 15–55 cm with surge duration of 0.8–3.1 days. Our field surveys revealed that the damage to coastal communities/structures was moderate although it caused severe flooding inland. We measured a maximum coastal wave runup of 4.3 m in Iwaisaki. Such a runup was smaller than that generated by other category 4 typhoons hitting Japan in the past. Our numerical model was able to reproduce the storm surge generated by the 2016 Typhoon Lionrock. This validated numerical model can be used in the future for typhoon-hazard studies along the coast of northeastern Japan. Despite relatively small surge/wave runups in coastal areas, Lionrock’s death toll was more than that of some other category 4 typhoons. We attribute this to various primary (e.g., flooding, surges, waves, strong winds) and secondary (e.g., landslides, coastal erosions, debris flows, wind-blown debris) mechanisms and their combinations and interactions that contribute to damage/death during a typhoon event.
Effects of breeding history and crop management on the root architecture of wheat
Authors: Fradgley N. , Evans G. , Biernaskie J.M. , Cockram J. , Marr E.C. , Oliver A. G. , Ober E. , Jones H. 
Published: June 2020

Abstract
Aims
Selection for optimal root system architecture (RSA) is important to ensure genetic gains in the sustainable production of wheat (Triticum aestivum L.). Here we examine the hypothesis that past wheat breeding has led to changes in RSA and that future breeding efforts can focus directly on RSA to improve adaptation to target environments.

Methods
We conducted field trials using diverse wheat varieties, including modern and historic UK varieties and non-UK landraces, tested under contrasting tillage regimes (non-inversion tillage versus conventional ploughing) for two trial years or different seeding rates (standard versus high rate) for one trial year. We used field excavation, washing and measurement of root crowns (‘shovelomics’) to characterise RSA traits, including: numbers of seminal, crown and nodal roots per plant, and crown root growth angle.

Results
We found differences among genotypes for all root traits. Modern varieties generally had fewer roots per plant than historic varieties. On average, there were fewer crown roots and root angles were wider under shallow non-inversion tillage compared with conventional ploughing. Crown root numbers per plant also tended to be smaller at a high seeding rate compared with the standard. There were significant genotype-by-year, genotype-by-tillage and genotype-by-seeding-rate interactions for many root traits.

Conclusions
Smaller root systems are likely to be a result of past selection that facilitated historical yield increases by reducing below-ground competition within the crop. The effects of crop management practices on RSA depend on genotype, suggesting that future breeding could select for improved RSA traits in resource-efficient farming systems.
Health-related quality of life and physical activity level after a behavior change program at Norwegian healthy life centers: a 15-month follow-up
Authors: Blom Ellen Eimhjellen , Aadland Eivind , Skrove Guri Kaurstad , Solbraa Ane Kristiansen , Oldervoll Line Merethe 
Published: June 2020

Abstract
Purpose
The long-term impact of primary care behavior change programs on health-related quality of life (HRQoL) and physical activity (PA) level is unknown. The aim of this study was to investigate changes in HRQoL and PA among participants after a 3-month behavior change intervention at Norwegian healthy life center (HLCs) and at a 15-month follow-up. Furthermore, we aimed to study associations between changes in PA and HRQoL.

Methods
We followed 524 adult participants (18–83 years), recruited from 32 HLCs in August 2016–January 2018, who provided data on HRQoL (SF-36) and PA (ActiGraph accelerometers) 12 months after a 3-month behavior change intervention. Changes in HRQoL and PA between baseline, 3-month and 15-month follow-ups, and associations between changes in PA and HRQoL were analyzed by linear mixed models.

Results
All HRQoL dimensions improved from baseline to 3-month follow-up, and the improvements maintained at 15-month follow-up (mean 3.1–13.1 points, p < 0.001). PA increased from baseline to 3 months (mean 418 steps/day, p < 0.001), but declined from 3 to 15 months (mean − 371 steps/day, p < 0.001). We observed positive associations between changes in PA and HRQoL (0.84–3.23 points per 1000 steps/day, p < 0.023).

Conclusions
Twelve months after completing a 3-month HLC intervention we found improved HRQoL, but not PA level. Still, there were positive associations between PA and HRQoL over this period, indicating that participants increasing their PA were more likely to improve their HRQoL.
GlycA, a novel marker for low grade inflammation, reflects gut microbiome diversity and is more accurate than high sensitive CRP in reflecting metabolomic profile
Authors: Mokkala Kati , Houttu Noora , Koivuniemi Ella , Sørensen Nikolaj , Nielsen Henrik Bjørn , Laitinen Kirsi 
Published: June 2020

Abstract
Introduction
Gut microbiota is, along with adipose tissue, recognized as a source for many metabolic and inflammatory disturbances that may contribute to the individual’s state of health.

Objectives
We investigated in cross-sectional setting the feasibility of utilizing GlycA, a novel low grade inflammatory marker, and traditional low grade inflammatory marker, high sensitivity CRP (hsCRP), in reflecting serum metabolomics status and gut microbiome diversity.

Methods
Fasting serum samples of overweight/obese pregnant women (n = 335, gestational weeks: mean 13.8) were analysed for hsCRP by immunoassay, GlycA and metabolomics status by NMR metabolomics and faecal samples for gut microbiome diversity by metagenomics. The benefits of GlycA as a metabolic marker were investigated against hsCRP.

Results
The GlycA concentration correlated with more of the metabolomics markers (144 out of 157), than hsCRP (55 out of 157) (FDR < 0.05). The results remained essentially the same when potential confounding factors known to associate with GlycA and hsCRP levels were taken into account (P < 0.05). This was attributable to the detected correlations between GlycA and the constituents and concentrations of several sized VLDL-particles and branched chain amino acids, which were statistically non-significant with regard to hsCRP. GlycA, but not hsCRP, correlated inversely with gut microbiome diversity.

Conclusion
GlycA is a superior marker than hsCRP in assessing the metabolomic profile and gut microbiome diversity. It is proposed that GlycA may act as a novel marker that reflects both the gut microbiome and adipose tissue originated metabolic aberrations; this proposal will need to be verified with regard to clinical outcomes.

Clinical trial registration
ClinicalTrials.gov, NCT01922791, August 14, 2013
Textual Representation and Intertextuality of Graphene in Swedish Newspapers
Authors: Boholm Max 
Published: June 2020

AbstractTextual representation of graphene in Sweden’s most circulated newspapers is analyzed in 229 articles from 2004 to 2018. What is and is not said about graphene is explored through systematically identifying the lexical and grammatical patterns of sentences using the word “graphene.” Graphene is said to be a super material with certain properties, to be an object of research, commercialization, and application, and to have societal significance. Given frequent classifications of graphene as a nanomaterial in scientific discourse, there is notably limited reference to graphene as “nano” in the newspapers and only marginal reference to risk. This paper discusses the findings regarding this Swedish newspaper discourse on graphene in relation to its intertextuality, i.e., how texts draw upon and recontextualize other texts: the Swedish newspaper discourse on graphene echoes discourses of promise formulated elsewhere in society; it is not very diversified in terms of themes; it is dominated by positive and neutral representations rather than by risk; and it makes limited reference to the nano-discourse, even though, according to most definitions, graphene is a nanomaterial.
Operationalising place for land system science
Authors: Switalski Michal , Grêt-Regamey Adrienne 
Published: June 2020

AbstractThe following paper introduces the concept of place for land system science to better understand how the transformation of place, as place-making, can be operationalised. The aim is to operationalise place with the motivation that a deeper understanding of people–place interactions can advance knowledge of land systems towards practicable solutions to current sustainability challenges. An overview of place studies spanning a wide range of research disciplines is presented to form a clear and concise theoretical foundation, necessary when operationalising place beyond its traditional research domains and applications. The limitations and potential of place in the context of land systems science are then explored through examples and the importance of operationalising place as both a product and process is demonstrated. Place and place-making are presented as a conceptual model, which allows for expansion and substantiation when deployed to relevant land system research tasks. In closing, the directions and key themes for further development of people–place interactions in land system science are discussed.
The Impact of Mitochondrial Dysfunction on Dopaminergic Neurons in the Olfactory Bulb and Odor Detection
Authors: Paß Thomas , Aßfalg Marlene , Tolve Marianna , Blaess Sandra , Rothermel Markus , Wiesner Rudolf J. , Ricke Konrad M. 
Published: June 2020

AbstractUnderstanding non-motor symptoms of Parkinson’s disease is important in order to unravel the underlying molecular mechanisms of the disease. Olfactory dysfunction is an early stage, non-motor symptom which occurs in 95% of Parkinson’s disease patients. Mitochondrial dysfunction is a key feature in Parkinson’s disease and importantly contributes to the selective loss of dopaminergic neurons the substantia nigra pars compacta. The olfactory bulb, the first olfactory processing station, also contains dopaminergic neurons, which modulate odor information and thereby enable odor detection as well as odor discrimination. MitoPark mice are a genetic model for Parkinson’s disease with severe mitochondrial dysfunction, reproducing the differential vulnerability of dopaminergic neurons in the midbrain. These animals were used to investigate the impact of mitochondrial dysfunction on olfactory-related behavior and olfactory bulb dopaminergic neuron survival. Odor detection was severely impaired in MitoPark mice. Interestingly, only the small anaxonic dopaminergic subpopulation, which is continuously replenished by neurogenesis, was moderately reduced in number, much less compared with dopaminergic neurons in the midbrain. As a potential compensatory response, an enhanced mobilization of progenitor cells was found in the subventricular zone. These results reveal a high robustness of dopaminergic neurons located in the olfactory bulb towards mitochondrial impairment, in striking contrast to their midbrain counterparts.