bluuebunny/crossref_metadata_2025_split huggingface dataset:
SGM: a novel time-frequency algorithm based on unsupervised learning improves high-frequency oscillation detection in epilepsy
Authors: Migliorelli Carolina , Bachiller Alejandro , Alonso Joan F , Romero Sergio , Aparicio Javier , Jacobs-Le Van Julia , Mañanas Miguel A , San Antonio-Arce Victoria 
Published: March 2020

Abstract

Objective. We propose a novel automated method called the S-Transform Gaussian Mixture detection algorithm (SGM) to detect high-frequency oscillations (HFO) combining the strengths of different families of previously published detectors. Approach. This algorithm does not depend on parameter tuning on a subject (or database) basis, uses time-frequency characteristics, and relies on non-supervised classification to determine if the events standing out from the baseline activity are HFO or not. SGM consists of three steps: the first stage computes the signal baseline using the entropy of the autocorrelation; the second uses the S-Transform to obtain several time-frequency features (area, entropy, and time and frequency widths); and in the third stage Gaussian mixture models cluster time-frequency features to decide if events correspond to HFO-like activity. To validate the SGM algorithm we tested its performance in simulated and real environments. Main results. We assessed the algorithm on a publicly available simulated stereoelectroencephalographic (SEEG) database with varying signal-to-noise ratios (SNR), obtaining very good results for medium and high SNR signals. We further tested the SGM algorithm on real signals from patients with focal epilepsy, in which HFO detection was performed visually by experts, yielding a high agreement between experts and SGM. Significance. The SGM algorithm displayed proper performance in simulated and real environments and therefore can be used for non-supervised detection of HFO. This non-supervised algorithm does not require previous labelling by experts or parameter adjustment depending on the subject or database considered. SGM is not a computationally intensive algorithm, making it suitable to detect and characterize HFO in long-term SEEG recordings.
An extract of protein pili and filtrat of human spermatozoa: A chemical review
Authors: Sukarjati S , Kusuma P S W , Binawati D K 
Published: March 2020

Abstract
This study aims to determine the effect of E. coli pili protein, filtrate media culture of E. coli and mixture of E. coli pili protein and E. coli filtrate on the motility and vitality of human sperm in an in vitro. The method for obtaining E. coli pili protein is E. coli prepared by do culture on TCG media to grow E. coli pili, then cutting the Pili E. coli using Omni mixer. Pili that has been obtained then dialysis, electrophoresis and electroelution. E. coli filtrate was obtained by the method of culturing E. coli on Mc Conkey media for 48 hours and then doing culture on BHI media for 48 hours. E. coli culture on BHI media was then centrifuged. E. coli cultures in BHI media were centrifuged and the supernatant produced from centrifugation was filtered using a. 0.22µm milipore filter. 10 samples of normal donor sperm according to 1999 WHO criteria, in preparation using Percoll method. Spermatozoa were incubated with E. coli pili protein, E. coli filtrate and a mixture of E. coli pili proteins and E. coli filtrate on U-base microplate. Incubation was performed for 1 hour. Observations by calculating the motility and vitality of spermatozoa each at 100 spermatozoa under a light microscope. Data analysis was done with SPSS. The results of this study found that Protein Pili E. coli, E. coli filtrate and a mixture of E. coli pili proteins and E. coli filtrate had significant effect on sperm motility (p = 0.000) and on sperm vitality (p = 0.000). Thus it can be concluded that E. coli pili protein and E. coli filtrate are potentially used for spermicidal materials.
Milk quality of Etawa crossbred dairy goat fed combination of fermented oil palm fronds, Tithonia (Tithonia diversifolia) and Elephant Grass (Pennisetum Purpureum)
Authors: Jamarun N , Pazla R , Zain M , Arief A 
Published: March 2020

Abstract
The aim of this research was to determine the influence of Fermented Oil Palm Fronds (FOPF) by Phanerochaete chrysosporium with Tithonia (T) and Elephant Grass (EG) as roughage on milk quality of Etawa Crossbreed Dairy Goats (ECDG). This study used a randomized complete design consisting of four treatment groups that is: T1 = 20% FOPF+16% T+64% EG, T2 = 20% FOPF+32% T+48% EG, T3 = 20% FOPF+48%T+32% EG and T4 = 20% FOPF+64% T+16% EG. Four replicates were used per treatment. The data were analysed using Analysis of Variance (ANOVA) and differences among the treatment means were tested using Duncan’s Multiple Range Tests (DMRT) with 5 replications and 1% confidence intervals. Parameters measured were quality of milk, i.e. protein, fat, lactose and mineral (Ca and P) content. Results showed using combination FOPF by phanerochaete chrysosporium with T and EG can significantly increase milk mineral concentration (Ca and P) (p<0.01), but there was no effect on milk content of protein, fat and laktosa (p>0.05). The combination FOPF by Phanerochaete chrysosporium with Tithonia (T) and Elephan Grass (EG) can be used as an alternative feed for goats. The best feed for dairy goats was 20% FOPF + 64 % T+ 16 % EG (Treatment D).
Mixed formula of neem leaves extract and curcumin as botanical insecticides for sustainable agriculture
Authors: Joeniarti E , Purwanti S , Hidayati S , Nurlina N 
Published: March 2020

Abstract
Neem extract possess important bioactivities such as insect repellent, antifeedant, growth inhibitor, and other insecticidal properties, while curcumin has many bioactivities as antioxidant, antiinflamation, antibacterial, as well as anticancer. Both natural substances were mixed as botanical insecticides formula. Its efficacy against bean pod sucking bugs in vitro were evaluated. Several stages were done in this research, i.e. neem leave extraction, curcumin extraction, mixed formula production, and efficacy evaluation of mixed formula against bean pod sucking bugs in laboratory. The result of research showed that the mixed formula of the botanical insecticides containing neem leaves extract and curcumin caused mortality of bean pod sucking bugs in vitro significantly. The mortality percentage of bugs was up to 96, 25%. Therefore, it is emphasized that mixed formula of botanical insecticides containing natural substances is an eco-friendly product for agriculture.
Oral supplementation of the Extract of Fish oil to reduce fasting blood Glucose and Endothel damage but not Malondialdehyde level in diabetic male Wistar Rat (Rattus norvegicus)
Authors: Sahadewa S , Durry F D , Pangkahila W , Pinatih G N I 
Published: March 2020

Abstract
The main target of hyperglycaemia is endothelial dysfunction involving pathways; protein kinase activation, hexosamine activation, polyol activation, and Advanced Glycation End Products (AGEs) formation, trigger reactive radical superoxide (O2•-) to stress oxidative. Malondialdehyde (MDA) is an end product of lipid peroxidation in body and is an indicator of oxidant-antioxidant level in diabetic patients. Fish oil composing mostly omega 3 as an antioxidant can reduce oxidative stress and hyperglycaemic condition. This study aimed to investigated the effects of omega-3-rich fish oil in lowering blood sugar levels, inhibiting oxidative stress and aortic endothelial cell damage in diabetic rat models. This study was an experimental study using post-test only control group design. Thirty-two rats divided into two study groups (n = 16 individuals per group), including the diabetic rat’s group (as control) and the diabetic rats group given fish oil doses of 300 mg/kilogram body weight/day. Provision of fish oil was performed for 28 days used Blackmores® fish oil. Blood sugar and malondialdehyde levels were analyzed by spectrophotometric method. The number of aortic endothelial cells was analyzed by haematoxylin-eosin staining. Comparability test showed that the average number of fasting blood glucose level after treatment in both groups showed highly significant differences (p=0.00). Although MDA level was reduced in treatment group than control group, but statistically not significantly difference, p=0.43. Comparability test showed that average of endothelial cell between control and treatment group significantly different (p=0.00). It was concluded that fish oil supplementation containing omega-3 in diabetic rats can lower blood glucose level and can inhibit endothelial cell damage.
Effect of oyster mushroom (Pleurotus ostreatus) to the mice longissimus dorsi muscle: A chemical review
Authors: Puspitasari R L , Pambudi A , Pangeran M H 
Published: March 2020

AbstractObjective of the research was to determine potency of oyster as mice nutrition growth. Oyster from market, oyster which harvested from ganyong waste biomass, and 6 weeks old male DDY strain mice were used for this research. The mushroom powder dissolved in distilled water then inserted orally force-fed 0.5 mL. There were 5 groups of treatments were dosage 200 mg/kgBW of oyster from market, without mushroom, dosage 100 mg/kgBW of mushroom from biomass waste of ganyong, dosage 200 mg/kgBW, and dosage 300 mg/kgBW. Parameters measured were histology and perimeter Longissimus dorsi muscle. The results showed histology analysis with higher dosage of mushroom solution could effect a better growth of Longissimus dorsi muscle.
The synergistic effects of 1, 2-epoxy-3 (3-(3, 4-dimethoxyphenyl)-4H-1-benzopiran-4on) propane and doxorubicin on breast cancer culture cell line
Authors: Dewi M K , Rusmartini T , Sobandi A F , Yuniarti L 
Published: March 2020

Abstract
Breast cancer is the second leading cause of death due to the low success rate of cancer treatment. Breast cancer therapy has not been maximized that encourage researchers to look for effective and selective cancer drugs. The purpose of this study was to determine at the synergistic effects of 1, 2-epoxy-3(3-(3, 4-dimethoxyphenyl)-4H-1-benzopiran-4on) propane (EPI) and doxorubicin on breast cancer cell cultures. This research is an experimental study in vitro, with a randomized study design post-test only control group design. The subject of this study was a T47D breast cancer cell culture, grown in a growth media with optimum time and incubation conditions. T47D are morphologically continuous cell lines like epithelial cells taken from breast tissue of a woman affected by ductal carcinoma. Cultures were performed using 30 wells, each well consisted of 1 x 106 cells. The results presented in the form of IC 50 using the Microculture Tetrazolium Salt (MTT) method and Combination index (CI) using compusyn software. An MTT method is a colorimetric assay based on cell metabolic activity. The results showed that the MTT method produced IC50 EPI values of 21.06μg/mL and doxorubicin 10.3μg/mL. EPI compounds in a combination of high concentrations have a CI below 1 (synergistic), whereas at high concentrations have CI above 1 (antagonistic). The conclusion of this study is that EPI compounds have weak anticancer in breast cancer and are synergistic with doxorubicin.
Unripe Papaya (Carica papaya L.) Aqueous Extract (UPAE) for the acute toxicity test on fatty liver changes
Authors: Kharisma Y , Rachmawati M , Indriyanti R A , Respati T 
Published: March 2020

Abstract
We investigated whether the oral ingestion of aqueous extract of unripe papaya fruit (UPAE), would also induce fatty liver changes by analysing the UPAE-treated mice qualitatively. This study used laboratory experiments which performed by a proposed (new) recommended method with 11 subjects of female mice which were administered with single oral doses of 0; 50; 200; 400; 800; 1, 000; 1, 500; 2, 000; 3, 000; 4, 000; 5, 000 mg/kg respectively. All these mice were euthanized after 24 hours of UPAE administration. Histopathological studies were conducted using liver samples which were stained by Haematoxylin-Eosin (HE). The UPAE did not cause death in all doses but did induce hepatic steatosis and steatohepatitis at 4, 000 and 5, 000 mg/kg dosages. Ballooning degeneration, necroinflammation and portal fibrosis revealed at 200 – 5, 000 mg/kg. These findings suggest that the UPAE provoked the fatty liver changes at the highest dose administration. It may be obtained from a multiple hit process by the accumulation of the phytochemical compounds of UPAE.
Waste processing technology for biodiesel fuel cooking oil using zeolite bayah catalyst
Authors: Fitriyah F , Pangesti F S P 
Published: March 2020

Abstract
The waste of household cooking oil is thrown away so that it causes environmental problems. Therefore, this problem must be responded to immediately, for example by processing household waste cooking oil into environmentally friendly biodiesel as an alternative method to address this problem. This study aims to make biodiesel from household waste cooking oil to eliminate or reduce levels of free fatty acids (ALB) contained in used cooking oil catalyst, namely Zeolite Bayah catalyst, while the temperature and reaction time is a fixed variable esterification reaction. The methods of this study are activated zeolite as a catalyst and mixed it with waste cooking oil. The results of this study indicate that the processing of used cooking oil using Zeolite Bayah catalyst can reduce the free fatty acid content contained in used cooking oil, and the physical characteristics of biodiesel generally meet the SNI-04-7182-2012 standard. So that it can be seen that the Zeolite Bayah catalyst influenced the process of reducing free fatty acids in used cooking oil, it can be concluded that some of the parameters are already included in the national standard 04-7182-2012, and it can be seen that the Zeolite Bayah catalyst density parameters obtained the results of 871, the density of NaOH catalysts obtained 862, while from the national standard 04-7182-2012, it was set at 850-890. Both of these results have met the standard for the quality of making biodiesel.
Test formulation of liquid organic fertilizer on growth and result of soybean plants
Authors: Herawati J , Indarwati I , Ernawati E 
Published: March 2020

Abstract
The objective of this research is to know the result of Liquid Organic Fertilizer (LOF) formulation on growth and yield of soybean crop. The hypothesis of this research is;(1) Suspected different LOF formulations provide different growth and yields of soybean crops;(2) suspected formulations F2 (LOF water hyacint + banana cobs) will have a better effect on the growth of soybean crop than other formulation;(3) suspected formulations F7 (LOF water hyacint + fish bone + chicken egg shell) will have a better effect on soybean crop yield than other formulations. The research was conducted by using Randomized Block Group Design with the following treatment: F = LOF Formulation; F0: without LOF (Control), F1: LOF water hyacinth (BioEcgon, F2 = BioEcgon + banana hump, F3 = BioEcgon + bone fish, F4 = BioEcgon + chicken egg shell, F5 = BioEcgon + banana hump + fish bone, F6 = BioEcgon + banana hump + chicken egg shell, F7 = BioEcgon + fish bone + chicken egg shell, andF8 = BioEcgon + banana hump + fish bone + chicken egg shell. From each treatment repeated 3 times, so it takes 27 plots of experiments (9x3 = 27) with a plot / plot of 18 m² (3x6). The parameters observed are plant height, number of leaves and dry weight of soybean seeds / ha. The data obtained were analyzed by Analysis of Variants (Anova), if there is a real difference followed by 5% BNT test. LOF application with various formulations is expected to improve the growth of soybean crops, so it can increase the yield of soy / ha, in the long term aims to increase land productivity and can prevent land degradation, as well as one solution in overcoming the problem of organic waste.
Local mineral formulas for supplementation of kacang goat’s feed based on wild forages, rice bran and sago stalk
Authors: Khalil K , Abbadiah A , Andri A , Evitayani E 
Published: March 2020

Abstract
Supplementation is required to satisfy mineral requirement of traditional grazing kacang goat. The present research was aimed to design of mineral supplement for better nutrition of kacang goat fed based on wild forages, rice bran and sago stalk. Crude nutrient and mineral composition of feed which are normally consumed or fed to kacang goat were analyzed for dry matter (DM), crude protein (CP), crude fibre (CF), and minerals (Ca, P). Samples of forages were collected from three areas, i.e. coconut plantations, banana plantations and idle lands. Mineral feed was then formulated by using locally available materials: Bukit Kamang’s stone powder, roasted fresh water mussel shell meal and bone ash. The local minerals were prepared in loose and block forms and offered to 12 young kacang goat males for 14 weeks in three treatments: P0: no supplementation (control), P1: supplemented with loose local mineral, and P2: supplemented with block local mineral. Each treatment consisted of 4 goats as replication. Parameters measured included: body weight gain, feed intake, feed conversion ratio (FCR), blood minerals (Ca, P), blood hematology (hemoglobin [HGB], mean corpuscular Hb concentration [MCHC], total red blood cell [RBC], white blood cell [WBC], hematocrit concentration [HCT]) and total protein. Results showed that there were seven kinds of wild forages consumed by grazing goat. The average crude nutrient and mineral content of the forages were as follows: DM: 17.2%, CP: 16.5%, CF: 18.20%; Ca and P: 4.5 dan 2.1g/kg DM, respectively. The average daily body weight gain was 35.4, 31.7 and 41.2 g/head, and FCR was 22.8, 26.0 and 21.3 for P0, P1 and P2, respectively. The mineral concentration, total protein and hematological values of blood of P2 ranged in normal values, except WBC. It was concluded that mineral supplementation in block lick form gave positive effect on growth performance and feed utilization efficiency.
Comparison of flavonoid from clove leaf oil cytotoxic activities with doxorubicin and cisplatin on liver cancer cell culture
Authors: Tejasari M , Respati T , Trusda S A D , Hendryanny E , Yuniarti L 
Published: March 2020

Abstract
In the development of new drugs as candidates for cancer therapy agents, cytotoxic testing is needed as an initial screening to determine the effect of a natural substance in inhibiting tumour cell growth. One of the active compounds of flavonoid derivatives that are being studied as anticancer is a compound derived from clove leaf oil. The purpose of this study was to determine the cytotoxic activity of flavonoid compounds from clove leaf oil on liver cancer cell culture and make comparisons with standard drugs for cancer therapy. Examination of cytotoxic activity was carried out on HepG2 cell line culture used MTT method. The absorbance of each well was measured with spectrophotometer at a wavelength of 595 nm. The absorbance results were calculated to create a cytotoxic curve. Based on the cytotoxic curve, IC50 values of flavonoid compounds, doxorubicin and cisplatin were 50.62μg/mL, 20.25μg/mL, 15.42μg/mL respectively. Those showed that flavonoid compounds from clove leaf oil have strong anti-cancer activity (IC50<100μg/mL) against liver cancer cells. It was concluded that flavonoid compounds isolated from clove leaf oil were shown to have anticancer activity on liver cancer cell and thus could be used as a new candidate for liver cancer therapy agents.
Antibacterial effect potention of n-hexane fraction of rose apple leaves
Authors: Suwendar S , Mulqie L , Choesrina R , Mardliyani D 
Published: March 2020

Abstract
This research was an attempt to prove scientifically about the antibacterial effect potention of n-hexane fraction of rose apple leaves [Eugenia aqueum (Burm. F) Alston]. The aims of this research were to determine the value of minimum inhibitory concentration (MIC) and type of effect as antibacterial from n-hexane fraction of rose apple leaves. Evaluation of antibacterial effect potention was carried out by diffusion method using well techniques on Staphylococcus aureus and Escherichia coli. Antibacterial effect was determined based on Bell’s criteria. The type of work against bacteria was evaluated by turbidimetry method. The results showed that the n-hexane fraction of rose apple leaves have antibacterial effect both against S. aureus and E. coli. The MIC of n-hexane fraction of rose apple leaves on S. aureus and E. coli were 1.56% and 6.25% respectively. The type of effect of n-hexane fraction of rose apple leaves were bactericidal both against S. aureus and E. coli.
Healthy home’s model for rural people with Analytical Hierarchy Process (AHP) in Kabupaten Ngawi, Indonesia
Authors: Rini T S , Rejeki F S , Purwaningrum S W , Wahyuningtyas E , Saurina N 
Published: March 2020

Abstract
For many years, the housing environment has been acknowledged as one of the main settings that affect human health. Living and housing conditions are the basis of many factors influencing residential health. Risk and environmental factors in house building that can affect the incidence of diseases and accidents include ventilation, lighting, density, humidity, animal transmitting diseases, clean water, household waste, to the inhabitants of the house. This study is to measure criteria for rural people in Kabupaten Ngawi, Indonesia, whether including healthy home or not using AHP. Parameter measurement of healthy home in this research developed parameter which have been done by Ministry of health department, which use three parameters namely the components of the house; sanitation facilities; the behaviour of the residents in healthy living. This study adds three parameters the behaviour in processing household waste; drainage; and physical path. These additional parameters have been approved by the head of the health office in Kabupaten Ngawi. This method uses analytic hierarchy process (AHP) to measure healthy home’s model result three values i.e. Lamda, Consistency Index, Consistency Ratio. The results show that healthy home for Kabupaten Ngawi with Global Priority value are 0.489792978, Medium Healthy Home with Global Priority value are 0.264061054 and last Unhealthy Home with Global Priority value are 0.246145969.
Durability based service life prediction of bacterial concrete
Authors: Prabowo H 
Published: March 2020

Abstract
Concrete durability is one the essential factors effecting the service life of structures. The problem in durability of structures not only gives great impact on the functionality of the structures themselves but also on their environment. The maintenance and repair process resulting from the problems will consume a lot of energy and resources. The use of bacteria as an effort for repairing concrete cracks becomes popular recently. Crack formation is a common problem in many concrete structures. Durability problems may rise due to these cracks usually reducing concrete integrity and permeability. There are many degradation mechanisms affecting concrete performance, two of them are carbonation and chloride ingress. Several researches prove increased resistance towards those two mechanisms. This paper is aimed to simulate service life of bacterial concrete based on carbonation and chloride ingress mechanisms incorporating carbonation rate constants and chloride migration coefficients. It is concluded that the utilization of bacteria in concrete is prospective in increasing the durability of concrete structures.