bluuebunny/crossref_metadata_2025_split huggingface dataset:
TNIK signaling imprints CD8+ T cell memory formation early after priming
Authors: Jaeger-Ruckstuhl Carla A. , Hinterbrandner Magdalena , Höpner Sabine , Correnti Colin E. , Lüthi Ursina , Friedli Olivier , Freigang Stefan , Al Sayed Mohamad F. , Bührer Elias D. , Amrein Michael A. , Schürch Christian M. , Radpour Ramin , Riether Carsten , Ochsenbein Adrian F. 
Published: April 2020

AbstractCo-stimulatory signals, cytokines and transcription factors regulate the balance between effector and memory cell differentiation during T cell activation. Here, we analyse the role of the TRAF2-/NCK-interacting kinase (TNIK), a signaling molecule downstream of the tumor necrosis factor superfamily receptors such as CD27, in the regulation of CD8+T cell fate during acute infection with lymphocytic choriomeningitis virus. Priming of CD8+T cells induces a TNIK-dependent nuclear translocation of β-catenin with consecutive Wnt pathway activation. TNIK-deficiency during T cell activation results in enhanced differentiation towards effector cells, glycolysis and apoptosis. TNIK signaling enriches for memory precursors by favouring symmetric over asymmetric cell division. This enlarges the pool of memory CD8+T cells and increases their capacity to expand after re-infection in serial re-transplantation experiments. These findings reveal that TNIK is an important regulator of effector and memory T cell differentiation and induces a population of stem cell-like memory T cells.
A solution-processable and ultra-permeable conjugated microporous thermoset for selective hydrogen separation
Authors: Liu Wei , Jiang Shu-Dong , Yan Youguo , Wang Wensen , Li Jing , Leng Kai , Japip Susilo , Liu Jiangtao , Xu Hai , Liu Yanpeng , Park In-Hyeok , Bao Yang , Yu Wei , Guiver Michael D. , Zhang Sui , Loh Kian Ping 
Published: April 2020

AbstractThe synthesis of a polymer that combines the processability of plastics with the extreme rigidity of cross-linked organic networks is highly attractive for molecular sieving applications. However, cross-linked networks are typically insoluble or infusible, preventing them from being processed as plastics. Here, we report a solution-processable conjugated microporous thermoset with permanent pores of ~0.4 nm, prepared by a simple heating process. When employed as a two-dimensional molecular sieving membrane for hydrogen separation, the membrane exhibits ultrahigh permeability with good selectivity for H2 over CO2, O2, N2, CH4, C3H6 and C3H8. The combined processability, structural rigidity and easy feasibility make this polymeric membrane promising for large-scale hydrogen separations of commercial and environmental relevance.
Functional identity enhances aboveground productivity of a coastal saline meadow mediated by Tamarix chinensis in Laizhou Bay, China
Authors: Yi Shijie , Wu Pan , Peng Xiqiang , Bai Fenghua , Gao Yanan , Zhang Wenxin , Du Ning , Guo Weihua 
Published: April 2020

AbstractResearch in recent decades has confirmed that biodiversity influences ecosystem productivity; however, the potential mechanisms regulating this process remain subject to controversy, due to variation across ecosystems. Here, the effects of biodiversity on ecosystem productivity were evaluated using three variables of biodiversity (taxonomic diversity, functional identity, and functional diversity) and surrounding environmental conditions in a coastal saline meadow located on the south coast of Laizhou Bay, China. At this site, the shrub and field layers were primarily dominated by Tamarix chinensis and natural mesic grasses, respectively. Our results showed that functional identity, which is quantified as the community weighted mean of trait values, had greater explanatory ability than taxonomic and functional diversity. Thus, ecosystem productivity was determined disproportionately by the specific traits of dominant species. T. chinensis coverage was a biotic environmental factor that indirectly affected ecosystem productivity by increasing the community weighted mean of plant maximum height, which simultaneously declined with species richness. The present study advances our understanding of the mechanisms driving variation in the productivity of temperate coastal saline meadows, providing evidence supporting the “mass ratio” hypothesis.
Development of a conductive biocomposite combining graphene and amniotic membrane for replacement of the neuronal network of tissue-engineered urinary bladder
Authors: Adamowicz J. , Pasternak I. , Kloskowski T. , Gniadek M. , Van Breda S. V. , Buhl M. , Balcerczyk D. , Gagat M. , Grzanka D. , Strupinski W. , Pokrywczynska M. , Drewa T. 
Published: April 2020

AbstractTissue engineering allows to combine biomaterials and seeded cells to experimentally replace urinary bladder wall. The normal bladder wall however, includes branched neuronal network propagating signals which regulate urine storage and voiding. In this study we introduced a novel biocomposite built from amniotic membrane (Am) and graphene which created interface between cells and external stimuli replacing neuronal network. Graphene layers were transferred without modifying Am surface. Applied method allowed to preserve the unique bioactive characteristic of Am. Tissue engineered constructs composed from biocomposite seeded with smooth muscle cells (SMC) derived from porcine detrusor and porcine urothelial cells (UC) were used to evaluate properties of developed biomaterial. The presence of graphene layer significantly increased electrical conductivity of biocomposite. UCs and SMCs showed an organized growth pattern on graphene covered surfaces. Electrical filed stimulation (EFS) applied in vitro led additionally to increased SMCs growth and linear arrangement. 3D printed chamber equipped with 3D printed graphene based electrodes was fabricated to deliver EFS and record pressure changes caused by contracting SMCs seeded biocomposite. Observed contractile response indicated on effective SMCs stimulation mediated by graphene layer which constituted efficient cell to biomaterial interface.
Amber from the Triassic to Paleogene of Australia and New Zealand as exceptional preservation of poorly known terrestrial ecosystems
Authors: Stilwell Jeffrey D. , Langendam Andrew , Mays Chris , Sutherland Lachlan J. M. , Arillo Antonio , Bickel Daniel J. , De Silva William T. , Pentland Adele H. , Roghi Guido , Price Gregory D. , Cantrill David J. , Quinney Annie , Peñalver Enrique 
Published: April 2020

AbstractThe Northern Hemisphere dominates our knowledge of Mesozoic and Cenozoic fossilized tree resin (amber) with few findings from the high southern paleolatitudes of Southern Pangea and Southern Gondwana. Here we report new Pangean and Gondwana amber occurrences dating from ~230 to 40 Ma from Australia (Late Triassic and Paleogene of Tasmania; Late Cretaceous Gippsland Basin in Victoria; Paleocene and late middle Eocene of Victoria) and New Zealand (Late Cretaceous Chatham Islands). The Paleogene, richly fossiliferous deposits contain significant and diverse inclusions of arthropods, plants and fungi. These austral discoveries open six new windows to different but crucial intervals of the Mesozoic and early Cenozoic, providing the earliest occurrence(s) of some taxa in the modern fauna and flora giving new insights into the ecology and evolution of polar and subpolar terrestrial ecosystems.
Rapid Identification of Candida Species in Candidemia Directly from Blood Samples Using Imperfect Match Probes
Authors: Higashi Yoshitsugu , Niimi Hideki , Sakamaki Ippei , Yamamoto Yoshihiro , Kitajima Isao 
Published: April 2020

AbstractCandidemia is associated with a high mortality rate, and initial adequate antifungal therapy results in a significant decrease in the crude mortality. We herein report a rapid method that can identify eight Candida species in candidemia using imperfect match quenching probes (IM Q-probes) within three and a half hours of whole blood sample collection. Furthermore, employing the D value, which reflects the difference between the Tm signature from a clinical isolate and that registered in the database, it is possible to quickly identify samples suitable for IM Q-probe identification. We first evaluated the method using 34 Candida colonies collected from different patients, and 100% (34/34) of the identification results matched the preidentified Candida species. We then performed blind tests using eight whole blood samples artificially mixed with eight different Candida species respectively, and all identification results correctly matched the preidentified Candida species. Finally, using 16 whole blood samples collected from candidemia patients, we compared the IM Q-probe method with the culture/sequencing method. Of a total of 16 patient samples, 100% (16/16) matched the culture and sequencing results. The IM Q-probe method is expected to contribute not only to the life expectancy of candidemia patients but also to antifungal stewardship.
Therapeutic effects of Low intensity extracorporeal low energy shock wave therapy (LiESWT) on stress urinary incontinence
Authors: Long Cheng-Yu , Lin Kun-Ling , Lee Yung-Chin , Chuang Shu-Mien , Lu Jian-He , Wu Bin-Nan , Chueh Kuang-Shun , Ker Chin-Ru , Shen Mei-Chen , Juan Yung-Shun 
Published: April 2020

AbstractThis study aimed to evaluate the therapeutic effects of Low intensity extracorporeal low energy shock wave therapy (LiESWT) on stress urinary incontinence (SUI). The investigation was a single-arm, open-label, multicentre study conducted in Taiwan. 50 female patients with SUI received LiESWT-treated with 0.25 mJ/mm2 intensity, 3000 pulses, and 3 pulses/second, once weekly for 4-weeks (W4) and 8-weeks (W8). The pad test, uroflowmetry, life quality questionnaires, and 3-day urinary diary measurement were performed before and after LiESWT intervention. The results revealed that 8-week of LiESWT treatment meaningfully improved urine leakage (pad test), maximum flow rate, post-voided residual urine, average urine volume, functional bladder capacity, urinary frequency, urgency symptom, and nocturia, which also persisted to show significant improvements at 1-month follow up (F1). Moreover, bothersome questionnaires scores were significantly improved at W4, W8, and F1 as compared to the baseline (W0). These results indicated that 8 weeks of LiESWT attenuated SUI symptoms on physical activity, reduced bladder leaks and overactive bladder (OAB), implying that LiESWT brought significant improvement in the quality of life. (ClinicalTrials.gov number, NCT04059133).
Chronology of cellular events related to mitochondrial burnout leading to cell death in Fuchs endothelial corneal dystrophy
Authors: Méthot Sébastien J. , Proulx Stéphanie , Brunette Isabelle , Rochette Patrick J. 
Published: April 2020

AbstractFuchs endothelial corneal dystrophy (FECD) is a degenerative eye disease characterized by corneal endothelial cell (CEC) death and the formation of guttae, an abnormal thickening of CEC’s basement membrane. At the tissue level, an oxidative stress causing mitochondrial damage and CEC death have been described to explain FECD pathogenesis. At the cellular level, our group has previously observed significant variability in the mitochondrial mass of FECD CECs. This led us to hypothesize that mitochondrial mass variability might play a key role in the chronology of events eventually leading to CEC death in FECD. We thus used different fluorescent markers to assess mitochondrial health and functionality as a function of mitochondrial mass in FECD corneal endothelial explants, namely, intra-mitochondrial calcium, mitochondrial membrane potential, oxidation level and apoptosis. This has led us to describe for the first time a sequence of events leading to what we referred to as a mitochondrial burnout, and which goes as follow. FECD CECs initially compensate for endothelial cell losses by incorporating mitochondrial calcium to help generating more ATP, but this leads to increased oxidation. CECs then resist the sustained need for more ATP by increasing their mitochondrial mass, mitochondrial calcium and mitochondrial membrane potential. At this stage, CECs reach their maximum capacity and start to cope with irreversible oxidative damage, which leads to mitochondrial burnout. This burnout is accompanied by a dissipation of the membrane potential and a release of mitochondrial calcium, which in turn leads to cell death by apoptosis.
MONOPOL - A traveling-wave magnetic neutron spin resonator for tailoring polarized neutron beams
Authors: Jericha Erwin , Gösselsberger Christoph , Abele Hartmut , Baumgartner Stefan , Berger Bernhard Maximilian , Geltenbort Peter , Hino Masahiro , Oda Tatsuro , Raab Robert , Badurek Gerald 
Published: April 2020

AbstractWe report on first experimental tests of a neutron magnetic spin resonator at a very cold neutron beam port of the high flux reactor at the ILL Grenoble. When placed between two supermirror neutron polarizers and operated in a pulsed traveling-wave mode it allows to decouple its time- and wavelength-resolution and can therefore be used simultaneously as electronically tunable monochromator and fast beam chopper. As a first ‘real’ scientific application we intend its implementation in the PERC (p roton and e lectron r adiation c hannel) project related to high-precision experiments in neutron beta decay.
Cell cycle–dependent localization of the proteasome to chromatin
Authors: Kito Yuki , Matsumoto Masaki , Hatano Atsushi , Takami Tomoyo , Oshikawa Kiyotaka , Matsumoto Akinobu , Nakayama Keiichi I. 
Published: April 2020

AbstractAn integrative understanding of nuclear events including transcription in normal and cancer cells requires comprehensive and quantitative measurement of protein dynamics that underlie such events. However, the low abundance of most nuclear proteins hampers their detailed functional characterization. We have now comprehensively quantified the abundance of nuclear proteins with the use of proteomics approaches in both normal and transformed human diploid fibroblasts. We found that subunits of the 26S proteasome complex were markedly down-regulated in the nuclear fraction of the transformed cells compared with that of the wild-type cells. The intranuclear proteasome abundance appeared to be inversely related to the rate of cell cycle progression, with restraint of the cell cycle being associated with an increase in the amount of proteasome subunits in the nucleus, suggesting that the nuclear proteasome content is dependent on the cell cycle. Furthermore, chromatin enrichment for proteomics (ChEP) analysis revealed enrichment of the proteasome in the chromatin fraction of quiescent cells and its apparent dissociation from chromatin in transformed cells. Our results thus suggest that translocation of the nuclear proteasome to chromatin may play an important role in control of the cell cycle and oncogenesis through regulation of chromatin-associated transcription factors.
Conditional down-regulation of GreA impacts expression of rRNA and transcription factors, affecting Mycobacterium smegmatis survival
Authors: Jha Rajiv Kumar , Udupa Shubha , Rai Ashutosh Kumar , Rani Phoolwanti , Singh Prakruti R. , Govind Shamitha , Nagaraja Valakunja 
Published: April 2020

AbstractGre, one of the conserved transcription factors in bacteria, modulates RNA polymerase (RNAP) activity to ensure processivity and fidelity of RNA synthesis. Gre factors regulate transcription by inducing the intrinsic-endonucleolytic activity of RNAP, allowing the enzyme to resume transcription from the paused and arrested sites. While Escherichia coli and a number of eubacteria harbor GreA and GreB, genus mycobacteria has a single Gre (GreA). To address the importance of the GreA in growth, physiology and gene expression of Mycobacterium smegmatis, we have constructed a conditional knock-down strain of GreA. The GreA depleted strain exhibited slow growth, drastic changes in cell surface phenotype, cell death, and increased susceptibility to front-line anti-tubercular drugs. Transcripts and 2D-gel electrophoresis (2D-PAGE) analysis of the GreA conditional knock-down strain showed altered expression of the genes involved in transcription regulation. Among the genes analysed, expression of RNAP subunits (β, β’ and ω), carD, hupB, lsr2, and nusA were affected to a large extent. Severe reduction in the expression of genes of rRNA operon in the knock-down strain reveal a role for GreA in regulating the core components of the translation process.
Prior Experience Alters the Appearance of Blurry Object Borders
Authors: Perez Diana C. , Cook Sarah M. , Peterson Mary A. 
Published: April 2020

AbstractObject memories activated by borders serve as priors for figure assignment: figures are more likely to be perceived on the side of a border where a well-known object is sketched. Do object memories also affect the appearance of object borders? Memories represent past experience with objects; memories of well-known objects include many with sharp borders because they are often fixated. We investigated whether object memories affect appearance by testing whether blurry borders appear sharper when they are contours of well-known objects versus matched novel objects. Participants viewed blurry versions of one familiar and one novel stimulus simultaneously for 180 ms; then made comparative (Exp. 1) or equality judgments regarding perceived blur (Exps. 2–4). For equivalent levels of blur, the borders of well-known objects appeared sharper than those of novel objects. These results extend evidence for the influence of past experience to object appearance, consistent with dynamic interactive models of perception.
TAS2R38 is a novel modifier gene in patients with cystic fibrosis
Authors: Castaldo Alice , Cernera Gustavo , Iacotucci Paola , Cimbalo Chiara , Gelzo Monica , Comegna Marika , Di Lullo Antonella Miriam , Tosco Antonella , Carnovale Vincenzo , Raia Valeria , Amato Felice 
Published: April 2020

AbstractThe clinical manifestation of cystic fibrosis (CF) is heterogeneous also in patients with the same cystic fibrosis transmembrane regulator (CFTR) genotype and in affected sibling pairs. Other genes, inherited independently of CFTR, may modulate the clinical manifestation and complications of patients with CF, including the severity of chronic sinonasal disease and the occurrence of chronic Pseudomonas aeruginosa colonization. The T2R38 gene encodes a taste receptor and recently its functionality was related to the occurrence of sinonasal diseases and upper respiratory infections. We assessed the T2R38 genotype in 210 patients with CF and in 95 controls, relating the genotype to the severity of sinonasal disease and to the occurrence of P. aeruginosa pulmonary colonization. The frequency of the PAV allele i.e., the allele associated with the high functionality of the T2R38 protein, was significantly lower in i) CF patients with nasal polyposis requiring surgery, especially in patients who developed the complication before 14 years of age; and ii) in CF patients with chronic pulmonary colonization by P. aeruginosa, especially in patients who were colonized before 14 years of age, than in control subjects. These data suggest a role for T2R38 as a novel modifier gene of sinonasal disease severity and of pulmonary P. aeruginosa colonization in patients with CF.
Dynamic input-dependent encoding of individual basal ganglia neurons
Authors: Matzner Ayala , Gorodetski Lilach , Korngreen Alon , Bar-Gad Izhar 
Published: April 2020

AbstractComputational models are crucial to studying the encoding of individual neurons. Static models are composed of a fixed set of parameters, thus resulting in static encoding properties that do not change under different inputs. Here, we challenge this basic concept which underlies these models. Using generalized linear models, we quantify the encoding and information processing properties of basal ganglia neurons recorded in-vitro. These properties are highly sensitive to the internal state of the neuron due to factors such as dependency on the baseline firing rate. Verification of these experimental results with simulations provides insights into the mechanisms underlying this input-dependent encoding. Thus, static models, which are not context dependent, represent only part of the neuronal encoding capabilities, and are not sufficient to represent the dynamics of a neuron over varying inputs. Input-dependent encoding is crucial for expanding our understanding of neuronal behavior in health and disease and underscores the need for a new generation of dynamic neuronal models.
The coupling of Phanerozoic continental weathering and marine phosphorus cycle
Authors: Wang Ruimin , Lang Xianguo , Ding Weiming , Liu Yarong , Huang Tianzheng , Tang Wenbo , Shen Bing 
Published: April 2020

AbstractOrganic matter production and decomposition primarily modulate the atmospheric O2 and CO2 levels. The long term marine primary productivity is controlled by the terrestrial input of phosphorus (P), while the marine P cycle would also affect organic matter production. In the past 540 million years, the evolution of terrestrial system, e.g. colonization of continents by vascular land plants in late Paleozoic, would certainly affect terrestrial P input into the ocean, which in turn might have impacted the marine primary productivity and organic carbon burial. However, it remains unclear how the marine P cycle would respond to the change of terrestrial system. Here we reconstruct the secular variations of terrestrial P input and biological utilization of seawater P in Phanerozoic. Our study indicates that riverine dissolved P input and marine P biological utilization (i.e. the fraction of P being buried as organophosphorus) are inversely correlated, suggesting the coupling of continental P input and marine P cycle. We propose an increase of P input would elevate surface ocean productivity, which in turn enhances marine iron redox cycle. Active Fe redox cycle favors the scavenging of seawater P through FeOOH absorption and authigenic phosphate formation in sediments, and accordingly reduces the bioavailability of seawater P. The negative feedback of marine P cycle to terrestrial P input would keep a relatively constant organic carbon burial, limiting the variations of surface Earth temperature and atmospheric O2 level.
Electron-polaron dichotomy of charge carriers in perovskite oxides
Authors: Husanu M.-A. , Vistoli L. , Verdi C. , Sander A. , Garcia V. , Rault J. , Bisti F. , Lev L. L. , Schmitt T. , Giustino F. , Mishchenko A. S. , Bibes M. , Strocov V. N. 
Published: April 2020

AbstractMany transition metal oxides (TMOs) are Mott insulators due to strong Coulomb repulsion between electrons, and exhibit metal-insulator transitions (MITs) whose mechanisms are not always fully understood. Unlike most TMOs, minute doping in CaMnO3 induces a metallic state without any structural transformations. This material is thus an ideal platform to explore band formation through the MIT. Here, we use angle-resolved photoemission spectroscopy to visualize how electrons delocalize and couple to phonons in CaMnO3. We show the development of a Fermi surface where mobile electrons coexist with heavier carriers, strongly coupled polarons. The latter originate from a boost of the electron-phonon interaction (EPI). This finding brings to light the role that the EPI can play in MITs even caused by purely electronic mechanisms. Our discovery of the EPI-induced dichotomy of the charge carriers explains the transport response of Ce-doped CaMnO3 and suggests strategies to engineer quantum matter from TMOs.