bluuebunny/crossref_metadata_2025_split huggingface dataset:
Szelenoészter-származékok daganatellenes és rezisztenciamódosító hatásának vizsgálata epidemiológiailag releváns daganatsejt-modellrendszerek felhasználásával
Authors: Gajdács Márió 
Published: September 2020

Absztrakt:
Bevezetés: A szerves szelénvegyületekről számos kutatás
bizonyítja, hogy képesek modulálni az emlőssejtek intracelluláris redox
homeosztázisát, így hatékony és szelektív daganatellenes szerek lehetnek, mivel
a daganatos sejtek érzékenyebbek a külső oxidatív stresszhatásokra.
Célkitűzés: Újszerű szerkezettel rendelkező szerves
szelénvegyületek daganatellenes és MDR-visszafordító hatásának vizsgálata
különböző daganatsejtes modellrendszerek felhasználásával in
vitro. Módszer: A vegyületek citotoxikus
aktivitását MTT módszerrel határoztuk meg, az ABCB1 efflux transzporter gátlását
rhodamin 123 akkumulációs vizsgálattal tanulmányoztuk áramlási citometriás
módszerrel. A vegyületek apoptózist indukáló hatását Annexin V-FITC módszerrel
határoztuk. OSIRIS Molecular Property Explorer és PreADMET 2.0 alkalmazásával
becsültük meg a vegyületek fizikai-kémiai és in vivo
abszorpciós tulajdonságait. Eredmények: Adott szerkezeti
elemekkel (négy vegyület: egy ciklusos szelenoanhidrid és három
alkil-keton-szelenoészter) rendelkező szelénvegyületek hatékonynak bizonyultak
minden vizsgálatban, míg mások csökkent vagy semmilyen aktivitást nem mutattak;
ezek a vegyületek erős citotoxikus hatásúak voltak a nanomoláris tartományban,
emellett hatékonyan gátolták az ABCB1 efflux pumpát. A vegyületek
lipofilitásának fontos szerepe van az effluxpumpa működésének befolyásolásában.
A ciklikus szelenoanhidrid hatékony korai apoptózist indukáló vegyület volt, míg
az alkil-keton szelenoészterek nekrotikus folyamatokat indukáltak.
Következtetések: Kísérleteink rávilágíttotak a szelén
központi szerepére a rákellenes és az MDR-visszafordító hatás szempontjából. A
vegyületek pontos molekuláris hatásmechanizmusa még nem ismert: azt
feltételezhetjük, hogy a szelénvegyületek aktivitása a szelenoészter csoport
hidrolíziséhez köthető. Eredményeink alapján az ígéretes vegyületek további
származékainak szintézise és biológiai hatásának vizsgálata indokolt lehet.
Efficacy assessment of levetiracetam monotherapy in newly-diagnosed epilepsy in adults using epileptiform activity index
Authors: Karlov V. A. , Kozhokaru A. B. , Vlasov P. N. , Samoilov A. S. , Udalov Yu. D. 
Published: September 2020

Abstract. Levetiracetam (LEV) is one of the most commonly prescribed antiepileptic drugs (AED). However, there were no studies on its efficacy and safety in terms of the correlation with epileptiform activity index (EAI) performed among the Russian population.Aim. To evaluate the efficacy and tolerability of LEV monotherapy in patients with newly-diagnosed epilepsy using epileptiform activity index (EAI) assessment.Materials and methods. The study included 107 patients (46 (43.0%) male and 61 (57.0%) female) with focal epilepsy (FE) (39.3%; n=42) or idiopathic generalized epilepsy (IGE) (60.7%; n=65). At each visit, video-electroencephalographic (video-EEG) monitoring was performed (baseline and in 1, 3, 6, and 12 months of the therapy). Therapeutic drug monitoring was performed at dose titration in 1 month of the therapy or in case of therapy correction. Treatment efficacy was assessed using the criteria of seizure absence (medically induced remission), seizure rate decrease by >50% (responders), seizure rate decrease by <50% – insufficient efficacy, a composite index of efficacy/tolerability (retention on treatment), and seizure rate increase compared to baseline and/or development of a new type of seizures (aggravation). Adverse events (AE) were assessed using the scale for side effects in AED treatment (SIDAED).Results. Total EAI at baseline was 5.2-fold higher in patients with IGE compared to FE patients (23.4±3.0 and 4.5±0.97, respectively). After 1 month of LEV therapy, EAI decreased to 3.4±1.1 and 1.9±0.4 in patients with IGE and FE, respectively (p<0.01). The decrease continued during the whole follow-up period. Retention on monotherapy was achieved in 82.2% (n=88/107) patients; in 87.6% (n=57/65) patients with IGE and in 73.8% (n=31/42) with FE. The rate of serious AEs during the follow-up period was 8.4% (n=9).Conclusions. LEV is an effective drug of choice for the initial treatment of newly-diagnosed FE and IGE in monotherapy along with a significant decrease in EAI. EAI is an objective measure of LEV treatment efficacy.
The role of susceptibility-weighted imaging (SWI) in neuroimaging in children with focal epilepsy
Authors: Polyanskaya M. V. , Demushkina A. A. , Kostylev F. A. , Vasilyev I. G. , Chadaev V. A. , Zavadenko N. N. , Alikhanov A. A. 
Published: September 2020

Aim. To approve of diagnostic effectiveness of SWAN (SWI) images in revealing of calcium containing epileptogenic substrates in children with resistant focal epilepsy.Materials and methods. The results of MRI in children with refractory focal epilepsy obtained in the Radiology Department of the Russian State Children Hospital in the period from 2018 to 2020 were observed retrospectively. High-resolution epileptological MR protocol used for investigation of 67 children. SWAN was applied in all cases for identification of calcium containing epileptogenic substrates, including cavernomas, DVA syndrome, cortical gangliogliomas, Sturge-Weber syndrome and tuberous sclerosis complex. All images were received by using MRI 3T 750 W Discovery GE.Results. In 17 cases (25%) SWAN provided important diagnostic information about the nature of the of epileptogenic lesion, its prevalence and borders. Additional earlier invisible structural changes were revealed in 2 cases of SWS and 1 cases of FCD; and in 13 cases SWAN gave us possibility to avoid CT for approving calcium in epileptogenic focus.Conclusion. We believe that adding SWAN in to the epileptological MR protocol is the necessary step for optimizing calcium and blood degradation products identification in the structure of potential epileptogenic focuses. Moreover, it would be very effective instrument for differential diagnosis of cerebral structural changes, specifying its etiology and, hence, would have influence on the therapeutic tactic and surgical strategy in children with focal epilepsy.
Bioethical management of patients with epilepsy: sex and age-related aspects
Authors: Mikhalovska-Karlova E. P. 
Published: September 2020

Aim. The study aimed to motivate doctors for ethical management of patients with epilepsy, to study the real picture of the application of values and principles of bioethics during the treatment of patients with epilepsy, and to develop an algorithm of ethical management of patients taking into account sex and age-related factors.Materials and Methods. Content analysis of historical documents and publications in Russian neurological journals was performed. The sociological study included observation, a questionnaire survey of post-graduate students and junior and senior students of medical institutions, standardized and non-standardized interviewing of 211 neurologists, and 265 pediatric neurologists.Results. In their clinical practice, 60% of the interviewed in 2017-2019 followed the principles of respect for the private autonomy of patients and their human dignity. 55% of responders followed partner and negotiation models of interaction with patients. More than 97% of respondents from these groups try to follow the principles of bioethics. However, more than 50% of them could not name classic and special principles of bioethics for their category of patients; 29% could not identify the type of model that they use in their work with patients; 75% of pediatric neurologists significantly lacked knowledge in the area of bioethics and practical skills in their application in patients’ management.Conclusion. The study revealed controversy between the increased motivation of doctors to ethical management and an insignificant level of knowledge in the area of bioethics and its practical implementation. To reduce this controversy, it is necessary to develop a system of continuous ethical education.
SUPPORTING DEVICES IN THE TREATMENT OF CHRONIC HEART FAILURE IN ELDERLY AND OLD PATIENTS
Authors: Hubulava Gennadiy , Kozlov Kirill L. , Bogomolov Andrey N. , Volkov Aleksey , Fedorets Viktor N. , Senkina Ekaterina Ivanovna 
Published: September 2020

Chronic heart failure (CHF) is a widespread disease associated with high rates of disability and mortality, as well as a decrease in the quality of life. Moreover, the vast majority of patients are elderly and senile. Modern surgical methods of treating heart failure are able to increase the duration and quality of life of such patients, however, the need far exceeds the volume of this care, and some highly effective methods common in Western countries are still not used in Russian clinical practice.
Elderly age is a risk factor for the development of senile asthenia (frailty) and concomitant pathology. Large abdominal surgery is often contraindicated for patients with signs of senile asthenia, and the method of choice in patients with severe heart failure is the implantation of devices for long-term mechanical circulatory support (LT-MCS). After implantation of LT-MCS, a regression of signs of senile asthenia may be observed.
The topic of an integrated approach to non-drug treatment of heart failure in elderly and senile patients in Russia has not been studied enough. In particular, the implantation of LT-MCS is not used in Russian clinical practice, while in many Western countries for many years it has been the main and most effective treatment for severe heart failure. Systematization of the available up-to-date information on this topic could help increase the duration and quality of life of patients with severe heart failure.
Vitamin D supplementation does not affect serum leptin and adiponectin levels in adults: A systematic review and meta-analysis of randomised controlled trials
Authors: Jafari-Sfidvajani Samira J , Jafari Fateme , Soltani Sepideh 
Published: September 2020

Background & aims: It is proposed that vitamin D supplementation might influence serum adipokines level; however, the recent meta-analyses have led to inconsistent results while they had methodological limitations. Therefore, this study aimed to examine the effects of vitamin D supplementation on serum adipokines through a systematic review and a meta-analysis of randomized placebo-controlled trials (RCTs) using a more comprehensive search strategy.
Methods: PubMed, Google Scholar, and Scopus were searched to identify related articles published up to November 2017. Mean±standard deviation (SD) of changes in serum adiponectin and leptin were extracted, and the effect sizes were pooled using a random-effects model. Studies with Controlled clinical trials design were eligible. Two reviewers extracted mean values and SDs of the baseline, final and net change values of leptin and adiponectin in the intervention and control groups.
Results: The pooled results indicated that vitamin D supplementation affects neither circulating leptin (Hedges’ g = 0.042, 95% CI: -0.294 to 0.378, p = 0. 0.807, n=15) nor adiponectin (Hedges’ g = -0.034, 95% CI: -0.243 to 0.174, p = 0.748, n=18) levels. Subgroup analysis showed that vitamin D supplementation might significantly decrease serum leptin level in patients with end stage renal disease (Hedges’ g = -0.634, 95 % CI: -1.221 to -0.047, p = 0.034).
Conclusions: Although the current evidence does not support the significant effect of vitamin D supplementation on adiponectin and leptin levels, further research is required to reach more definitive conclusions.
Inquiring pre-service teachers’ narratives on language policy and identity during their practicum.
Authors: Montoya-López Juan Carlos , Mosquera-Andrade Ayda Vanessa , Peláez-Henao Oscar Alberto 
Published: September 2020

This narrative inquiry study aims to unveil the incorporation of policy agency within the construction of teacher identity of pre-service teachers in their academic practicums. Drawing on a critical-sociocultural approach to narrative inquiry, language policy, and teacher identity, the narratives of five students of an English teaching program in Medellín, Colombia, were examined. Their reflections and decision making on foreign language policies regulating their pedagogical practices at various schools show their social and critical awareness. Teaching represents a high moral load for them as they embrace a humanistic perspective. However, their narratives also pose challenges to language teaching programs in helping pre-service teachers to build micropolitical agency supported on solid theoretical knowledge to participate in policymaking. On the one hand, their narrations of the policy appropriation process they undertook show their frustration and disappointment in trying to participate when policy structures and other policy arbiters were close to them. On the other hand, when policy structures and arbiters openly allowed their policy participation, their actions and reflections focused on methodological concerns but rarely addressed social or critical awareness regarding curriculum design and development. Therefore, supporting pre-service teachers in strengthening their identities with solid theoretical constructs should be a priority because they will build micropolitical agency to overcome political tensions and negotiate their policy participation.
Gradient Boosting for Parkinson’s Disease Diagnosis from Voice Recordings
Authors: Karabayir Ibrahim , Goldman Samuel , Pappu Suguna , Akbilgic Oguz 
Published: September 2020

Abstract
Background: Parkinson’s Disease (PD) is a clinically diagnosed neurodegenerative disorder that affects both motor and non-motor neural circuits. Speech deterioration (hypokinetic dysarthria) is a common symptom, which often presents early in the disease course. Machine learning can help movement disorders specialists improve their diagnostic accuracy using non-invasive and inexpensive voice recordings.Method: We used “Parkinson Dataset with Replicated Acoustic Features Data Set” from the UCI-Machine Learning repository. The dataset included 44 speech-test based acoustic features from patients with PD and controls. We analyzed the data using various machine learning algorithms including Light and Extreme Gradient Boosting, Random Forest, Support Vector Machines, K-nearest neighborhood, Least Absolute Shrinkage and Selection Operator Regression, as well as logistic regression. We also implemented a variable importance analysis to identify important variables classifying patients with PD. Results: The cohort included a total of 80 subjects: 40 patients with PD (55% men) and 40 controls (67.5% men). Disease duration was 5 years or less for all subjects, with a mean Unified Parkinson’s Disease Rating Scale (UPDRS) score of 19.6 (SD 8.1), and none were taking PD medication. The mean age for PD subjects and controls was 69.6 (SD 7.8) and 66.4 (SD 8.4), respectively. Our best-performing model used Light Gradient Boosting to provide an AUC of 0.951 with 95% confidence interval 0.946-0.955 in 4-fold cross validation using only seven acoustic features.Conclusions: Machine learning can accurately detect Parkinson’s disease using an inexpensive and non-invasive voice recording. Light Gradient Boosting outperformed other machine learning algorithms. Such approaches could be used to inexpensively screen large patient populations for Parkinson’s disease.
Upregulation of long non-coding RNA LOC284454 may serve as a new serum biomarker for head and neck cancers
Authors: Fan Chunmei , Wang Jinpeng , Tang Yanyan , Zhang Shanshan , Xiong Fang , Guo Can , Zhou Yanhong , Li Zheng , Li Xiaoling , Li Yong , Li Guiyuan , Zeng Zhaoyang , Xiong Wei 
Published: September 2020

Abstract
Background: Identification of effective diagnostic and prognostic biomarkers of cancer is necessary for improving precision medicine. Long non-coding RNAs (lncRNAs) play an important regulatory role in tumor initiation and progression. The lncRNA LOC284454 is distinctly expressed in various head and neck cancers (HNCs), as demonstrated by our previous bioinformatics analysis. However, the expression levels and functions of LOC284454 in cancer are still unclear.Methods: We investigated the dysregulation of lncRNAs in HNCs using the GEO database and found that LOC284454 was highly expressed in HNCs. Serum samples from 212 patients with HNCs and 121 normal controls were included in this biomarker study. We measured the expression of LOC284454 in the sera of HNC patients and normal controls using RT-qPCR. Receiver operating characteristics (ROC) analysis is an important statistical method that is widely used in clinical diagnosis and disease screening. ROC was used to analyze the clinical value of LOC284454 in the early diagnosis of HNCs.Results: LOC284454 was significantly upregulated in the sera of patients with nasopharyngeal carcinoma, oral cancer, and thyroid cancer. LOC284454 upregulation had good clinical diagnostic value in these cancers, as evaluated by area under the ROC curve values of 0.931, 0.698, and 0.834, respectively.Conclusions:LOC284454 may be a valuable serum biomarker for HNCs facilitating the early diagnosis of malignant cancers. Further studies are needed to elucidate the mechanisms underlying the involvement of LOC284454 in HNCs. This study provides the first evidence that LOC284454 may be a serum biomarker for HNCs.
Diagnosis of left ventricular hypertrophy using convolutional neural network
Authors: Jian Zini , Wang Xianpei , Zhang Jingzhe , Wang Xinyu , Deng Youbin 
Published: September 2020

Abstract
Background: Clinically, doctors obtain the left ventricular posterior wall thickness (LVPWT) mainly by observing ultrasonic echocardiographic video stream to capture a single frame of images with diagnostic significance, and then mark two key points on both sides of the posterior wall of the left ventricle with their own experience for computer measurement. In the actual measurement, the doctor's selection point is subjective, and difficult to accurately locate the edge, which will bring errors to the measurement results.Methods: In this paper, a convolutional neural network model of left ventricular posterior wall positioning was built under the TensorFlow framework, and the target region images were obtained after the positioning results were processed by non-local mean filtering and opening operation. Then the edge detection algorithm based on threshold segmentation is used. After the contour was extracted by adjusting the segmentation threshold through prior analysis and the OTSU algorithm, the design algorithm completed the computer selection point measurement of the thickness of the posterior wall of the left ventricle. Results: The proposed method can effectively extract the left ventricular posterior wall contour and measure its thickness. The experimental results show that the relative error between the measurement result and the hospital measurement value is less than 15%, which is less than 20% of the acceptable repeatability error in clinical practice. Conclusions: Therefore, the measurement method proposed in this paper has the advantages of less manual intervention, and the processing method is reasonable and has practical value.
Discovery and validation of mucosal TNF expression combined with histological score - a biomarker for personalized treatment in ulcerative colitis
Authors: Florholmen Jon R. , Johnsen Kay-Martin , Renate Meyer , Olsen Trine , Moe Øystein Kittel , Tandberg Petter , Gundersen Mona D. , Kvamme Jan-Magnus , Johnsen Knut , Løitegård Terje , Raschpichler Gabriele , Vold Cecilia , Sørbye Sveinung W. , Goll Rasmus 
Published: September 2020

Abstract
Background There are no accurate markers that can predict clinical outcome in ulcerative colitis at time of diagnosis. The aim of this study was to explore a comprehensive data set to identify and validate predictors of clinical outcome in the first year following diagnosis. Methods Treatment naive-patients with ulcerative colitis were included at time of initial diagnosis from 2004-2014, followed by a validation study from 2014-2018. Patients were treated according to clinical guidelines following a standard step-up regime. Patients were categorized according to the treatment level necessary to achieve clinical remission: mild, moderate and severe. The biopsies were assessed by Robarts histopathology index (RHI) and TNF gene transcripts. Results We included 66 patients in the calibration cohort and 89 patients in the validation. Mucosal TNF transcripts showed high test reliability for predicting severe outcome in UC. When combined with histological activity (RHI) scores the test improved its diagnostic reliability. Based on the cut-off values of mucosal TNF and RHI scores from the calibration cohort, the combined test had still high reliability in the validation cohort (specificity 0.99, sensitivity 0.44, PPV 0.89, NPV 0.87) and a diagnostic odds-ratio (DOR) of 54.Conclusions The combined test using TNF transcript and histological score at debut of UC can predict severe outcome and the need for anti-TNF therapy with a high level of precision. These validated data may be of great clinical utility and contribute to a personalized medical approach with the possibility of top-down treatment for selected patients.
Incidence and risk factor for short term postoperative cough after thyroidectomy
Authors: Wu Junfu , Dai Liyuan , Lou Weihua 
Published: September 2020

Abstract
Background: The prevalence of potential risk factors for postoperative cough after thyroidectomy remain unknown. The current study aimed to research postoperative cough in patients undergoing thyroid surgery prospectively.Methods: Adult patients who underwent primary thyroid surgery were selected prospectively. Data regarding age, sex, BMI, pathology and surgical procedure were collected and analyzed. The Leicester Cugh Questionnaire (LCQ) was required to be completed by all patients before operation, 2 weeks and 4 weeks after operation.Results: There were 1264 patients enrolled in total. Eleven patients with vocal cord paralysis were excluded. In patients with benign disease, postoperative cough occurred in 61 patients, with an prevalence rate of 17. 0% compared to an prevalence rate of 33.1% in patients with malignant disease; the difference was significant. For benign patients, the factors of smoking and operation time were independently related to the occurrence of postoperative cough. For malignant patients, the factors of smoking, operation time, operation extent, and the number of positive nodes at level 6 were independently related to the occurrence of postoperative cough. There was no significant difference regarding the LCQ score in patients with benign or malignant disease at the preoperative and the postoperative 4-week time periods. Patients with malignant disease had a significantly lower LCQ score than patients with benign disease at the postoperative 2-week time point (p=0.004).Conclusions: Patients undergoing thyroid cancer surgery had a higher incidence of postoperative cough and were also associated with a decreased cough-related quality of life. The factors of smoking and operation time were the most important predictors for postoperative cough after thyroidectomy.