bluuebunny/crossref_metadata_2025_split huggingface dataset:
Not only compulsivity: The SAPAP3-KO mouse reconsidered as a comorbid model expressing a spectrum of pathological repetitive behaviors
Authors: Lamothe H , Schreiweis C , Lavielle O , Mallet L , Burguière E 
Published: January 2020

AbstractSymptom comorbidity is present amongst neuropsychiatric disorders with repetitive behaviours, complicating clinical diagnosis and impeding appropriate treatments. This is of particular importance for obsessive-compulsive disorder (OCD) and Tourette syndrome. Here, we meticulously analysed the behaviour of Sapap3 knockout mice, the recent rodent model predominantly used to study compulsive-like behaviours, and found that its behaviour is more complex than originally and persistently described. Indeed, we detected previously unreported elements of distinct pathologically repetitive behaviours, which do not form part of rodent syntactic cephalo-caudal self-grooming. These repetitive behaviours include sudden, rapid body and head twitches, resembling tic-like movements. We also observed that another type of repetitive behaviours, aberrant hindpaw scratching, is responsible for the flagship-like skin lesions of this mouse model, rather than originally suspected self-grooming. In order to characterize the symptomatological nature of observed repetitive behaviours, we pharmacologically challenged these phenotypes by systemic aripiprazole administration, a first-line treatment for tic-like symptoms in Tourette Syndrome and trichotillomania. A single treatment of aripiprazole significantly reduced the number of tic-like movements but not syntactic grooming events. These observations are in line with the high comorbidity of tic- and compulsive-like symptoms in Tourette, OCD and trichotillomania patients as well as with the hypothesis of shared neurobiological mechanisms.
Structured clustering of the glycosphingolipid GM1 is required for membrane curvature induced by cholera toxin
Authors: Kabbani Abir Maarouf , Raghunathan Krishnan , Lencer Wayne I. , Kenworthy Anne K. , Kelly Christopher V. 
Published: January 2020

ABSTRACTAB5bacterial toxins and polyomaviruses induce membrane curvature as a mechanism to facilitate their entry into host cells. How membrane bending is accomplished is not yet fully understood but has been linked to the simultaneous binding of the pentameric B-subunit to multiple copies of their glycosphingolipid receptors. Here, we probe the toxin membrane binding and internalization mechanisms by using a combination of super-resolution and polarized localization microscopy. We show that cholera toxin subunit B (CTxB) can induce membrane curvature only when bound to multiple copies of its glycosphingolipid receptor, GM1, and the ceramide structure of GM1 is likely not a determinant of this activity as assessed in model membranes. A mutant CTxB capable of binding only a single GM1 fails to generate curvature either in model membranes or in cells and clustering the mutant CTxB-single-GM1 complexes by antibody cross-linking does not rescue the membrane curvature phenotype. We conclude that both the multiplicity and specific geometry of GM1 binding sites are necessary for the induction of membrane curvature. We expect this to be a general rule of membrane behavior for all AB5toxins and polyomaviruses that bind glycosphingolipids to invade host cells.SIGNIFICANCE STATEMENTMembrane binding toxins demonstrate both a public health challenge and a bioengineering opportunity due to their efficient internalization into cells. These toxins multivalently bind to naturally occurring lipid receptors at the plasma membrane and initiate endocytosis. This manuscript reports the importance of structured lipid-receptor clustering for the induction of membrane bending. We also observed that the magnitude of membrane curvature was correlated to the stoichiometry of toxin-bound receptors. By identifying how these bacterial proteins initiate membrane curvature, these findings provide mechanistic insights into the early steps of pathogenic endocytosis.
Anillin propels myosin-independent constriction of actin rings
Authors: Kučera Ondřej , Janda Daniel , Siahaan Valerie , Dijkstra Sietske H. , Pilátová Eliška , Zatecka Eva , Diez Stefan , Braun Marcus , Lansky Zdenek 
Published: January 2020

AbstractConstriction of the actin cytokinetic ring is an essential step of cell division. In a generally accepted view, the constriction is driven by relative sliding of actin filaments propelled by myosin motors. However, in multiple organisms, the ring constriction is myosin independent. How actin rings constrict in the absence of motor activity remains unclear. Here, we demonstrate that actin contractility can be propelled by anillin, a diffusible non-motor actin crosslinker, localising to the cytokinetic ring. We in vitro observed the formation and constriction of rings comprising multiple actin filaments bundled by anillin. Rings constricted due to anillin-generated forces maximising the overlap lengths between the filaments. Actin disassembly promoted constriction. We propose that actin crosslinkers, generating forces complementary to molecular motors, contribute to the contractility of diverse actin structures, including the cytokinetic ring.
Using visual encounter data to improve capture-recapture abundance estimates
Authors: Joseph Maxwell B. , Knapp Roland A. 
Published: January 2020

AbstractCapture-recapture studies are widely used in ecology to estimate population sizes and demographic rates. In some capture-recapture studies, individuals may be visually encountered but not identified. For example, if individual identification is only possible upon capture and individuals escape capture, visual encounters can result in failed captures where individual identities are unknown. In such cases, the data consist of capture histories with known individual identities, and counts of failed captures for individuals with unknown identities. These failed captures are ignored in traditional capture-recapture analyses that require known individual identities. Here we show that if animals can be encountered at most once per sampling occasion, failed captures provide lower bounds on population size that can increase the precision of abundance estimates. Analytical results and simulations indicate that visual encounter data improve abundance estimates when capture probabilities are low, and when there are few repeat surveys. We present a hierarchical Bayesian approach for integrating failed captures and auxiliary encounter data in statistical capture-recapture models. This approach can be integrated with existing capture-recapture models, and may prove particularly useful for hard to capture species in data-limited settings.
Behavioural traits and feeding ecology of Mediterranean lionfish and native species naiveté to lionfish predation
Authors: D’Agostino Daniele , Jimenez Carlos , Reader Tom , Hadjioannou Louis , Heyworth Stephanie , Aplikioti Marilena , Argyrou Marina , Feary David A. 
Published: January 2020

ABSTRACTThe detrimental effects of invasion by Indo-Pacific lionfish (Pterois volitans/miles) on western Atlantic fishes have spurred concerns for Mediterranean fish biodiversity, where a Lessepsian invasion of lionfish has recently begun. In order to assess the potential impact on biodiversity, we examine key behavioural and ecological traits of lionfish, and the resident fish community in the Mediterranean, that may contribute to lionfish invasion success. We focus on Cyprus, where lionfish populations were first sighted in 2012 and have now established abundant and stable populations. Using field observations, we examine lionfish predatory behaviour and feeding ecology, and resident fish species naiveté to hunting lionfish. Our findings suggest that lionfish in the Mediterranean are crepuscular generalist predators, with prey targeted dominated by small-bodied benthic or bentho-pelagic associated species. Such prey are more likely to be native than introduced (Lessepsian) fishes, with native prey fishes showing greater naiveté towards lionfish than Lessepsian prey species. Notably, one of the Mediterranean’s key ecological fish species (the native damselfishChromis chromis), showed the highest level of naiveté and was the most heavily targeted prey. Overall, lionfish in the Mediterranean show similar predatory behaviour and ecology to their western Atlantic counterparts. Although the Mediterranean invasion is still relatively recent, it may result in a similar disruption to reef fish biomass to that recorded in the Atlantic, with impact to the structure and biodiversity of reef fish communities and the services they provide.
The effect of genetic architecture and selfing on the capacity of a population to expand its range
Authors: Eriksson Martin , Rafajlović Marina 
Published: January 2020

AbstractPrevious theoretical work on range expansions over heterogeneous environments showed that there is a critical environmental gradient where range expansion stops. For populations with freely recombining loci underlying the trait under selection (hereafteradaptive loci), the critical gradient in one-dimensional habitats depends on the fitness cost of dispersal, and the strength of selection relative to genetic drift. Here, we extend the previous work in two directions and ask: What is the role of the genetic architecture of adaptive loci during range expansions? And what effect does the ability of selfing have on range expansions? To answer these questions, we use computer simulations. We demonstrate that, while reduced recombination rates between adaptive loci slow down range expansions due to poor purging of locally deleterious alleles at the expansion front, they may also allow a species to occupy a greater range. In fact, for some parameter values, we find that a population with freely recombining adaptive loci experiences global extinction, whereas reduced recombination rates allow for a successful expansion over a wide geographic range. In addition, we find that allowance of selfing may improve the ability of populations to expand their ranges. We discuss the mechanisms underlying these results.
Functional assessment of cell entry and receptor usage for lineage B β-coronaviruses, including 2019-nCoV
Authors: Letko Michael , Munster Vincent 
Published: January 2020

AbstractOver the past 20 years, several coronaviruses have crossed the species barrier into humans, causing outbreaks of severe, and often fatal, respiratory illness. Since SARS- CoV was first identified in animal markets, global viromics projects have discovered thousands of coronavirus sequences in diverse animals and geographic regions. Unfortunately, there are few tools available to functionally test these novel viruses for their ability to infect humans, which has severely hampered efforts to predict the next zoonotic viral outbreak. Here we developed an approach to rapidly screen lineage B betacoronaviruses, such as SARS-CoV and the recent 2019-nCoV, for receptor usage and their ability to infect cell types from different species. We show that host protease processing during viral entry is a significant barrier for several lineage B viruses and that bypassing this barrier allows several lineage B viruses to enter human cells through an unknown receptor. We also demonstrate how different lineage B viruses can recombine to gain entry into human cells and confirm that human ACE2 is the receptor for the recently emerging 2019-nCoV.
Stator–rotor interaction in the tip leakage flow of an inlet vaned low-speed axial fan
Authors: Vazquez Gonzalez Adrián , Meana-Fernández Andrés , Fernández Jesús Manuel 
Published: January 2020

Purpose
The purpose of the paper is to quantify the impact of the non-uniform flow generated by the upstream stator on the generation and convection of the tip leakage flow (TLF) structures in the passages of the rotor blades in a low-speed axial fan.

Design/methodology/approach
A full three dimensional (3D)-viscous unsteady Reynolds-averaged Navier-stokes (RANS) (URANS) simulation of the flow within a periodic domain of the axial stage has been performed at three different flow rate coefficients (φ = 0.38, 0.32, 0.27) using ReNormalization Group k-ε turbulence modelling. A typical tip clearance of 2.3 per cent of the blade span has been modelled on a reduced domain comprising a three-vaned stator and a two-bladed rotor with circumferential periodicity. A non-conformal grid with hybrid meshing, locally refined O-meshes on both blades and vanes walls with (100 × 25 × 80) elements, a 15-node meshed tip gap and circumferential interfaces for sliding mesh computations were also implemented. The unsteady motion of the rotor has been covered with 60 time steps per blade event. The simulations were validated with experimental measurements of the static pressure in the shroud of the blade tip region.

Findings
It has been observed that both TLF and intensities of the tip leakage vortex (TLV) are significantly influenced by upstream stator wakes, especially at nominal and partial load conditions. In particular, the leakage flow, which represents 12.4 per cent and 11.3 per cent of the working flow rate, respectively, has shown a clear periodic fluctuation clocked with the vane passing period in the relative domain. The periodic fluctuation of the TLF is in the range of 2.8-3.4 per cent of the mean value. In addition, the trajectory of the tip vortex is also notably perturbed, with root-mean squared fluctuations reaching up to 18 per cent and 6 per cent in the regions of maximum interaction at 50 per cent and 25 per cent of the blade chord for nominal and partial load conditions, respectively. On the contrary, the massive flow separation observed in the tip region of the blades for near-stall conditions prevents the formation of TLV structures and neglects any further interaction with the upstream vanes.

Research limitations/implications
Despite the increasing use of large eddy simulation modelling in turbomachinery environments, which requires extremely high computational costs, URANS modelling is still revealed as a useful technique to describe highly complex viscous mechanisms in 3D swirl flows, such as unsteady tip flow structures, with reasonable accuracy.

Originality/value
The paper presents a validated numerical model that simulates the unsteady response of the TLF to upstream perturbations in an axial fan stage. It also provides levels of instabilities in the TLV derived from the deterministic non-uniformities associated to the vane wakes.
Second law analysis on Hall effect of natural convection flow through vertical channel in the presence of uniform heat source/sink
Authors: Roja A. , Gireesha B.J. 
Published: January 2020

Purpose
Microfluidics is one of the extensive elaborated technologies in thermal and engineering fields due to its wide range of applications, such as micro heat exchangers, micro mixture and microchannel heat sinks, which is used to develop a large number of microscopic devices and systems. Enhancement of thermal energy using verity of nanoliquids is one of the challenges in these applications of microfluidics. Therefore, using single wall carbon nanotubes for enhancement of thermal energy in microchannel is the main purpose of this study. Hall effect of natural convection flow in a vertical channel with slip and temperature jump condition is considered. The impacts of radiative heat flux, uniform heat source/sink, viscous dissipation and joule heating are also taken into account.

Design/methodology/approach
Suitable non-dimension variables are applied to the governing equations to reduce the system into ordinary differential equations. The reduced nonlinear system is then solved numerically using Runge–Kutta–Fehlberg fourth–fifth-order method along with shooting technique. The impact of different pertinent parameters on numerical solutions of primary velocity, secondary velocity, temperature, entropy generation and Bejan number is comprehensively discussed in detail. Also, the obtained numerical results are compared with existing one which perfectly found to be in good agreement.

Findings
It is established that, with the aspects of Joule heating, viscous dissipation, radiative heat flux and uniform heat source/sink, the production in the entropy can be improved. Further, it is found that the increasing ratio of wall ambient temperature difference and nanoparticle volume fraction leads to enhance the entropy generation. The same effect reverses with increasing values of fluid wall interaction parameter (FWIP) and rare faction. The irreversibility ratio enhances with larger values of nanoparticle volume fraction and decelerates with increment values of FWIP.

Originality/value
The impact of single wall carbon nanoliquid in a vertical channel flow by using radiative heat flux, heat source/sink, joule heating and viscous dissipation is first time investigated. Further, the influence of Hall current is explored in detail.
The hunt for computerized support in information security policy management
Authors: Rostami Elham , Karlsson Fredrik , Kolkowska Ella 
Published: January 2020

Purpose
The purpose of this paper is to survey existing information security policy (ISP) management research to scrutinise the extent to which manual and computerised support has been suggested, and the way in which the suggested support has been brought about.

Design/methodology/approach
The results are based on a literature review of ISP management research published between 1990 and 2017.

Findings
Existing research has focused mostly on manual support for managing ISPs. Very few papers have considered computerised support. The entire complexity of the ISP management process has received little attention. Existing research has not focused much on the interaction between the different ISP management phases. Few research methods have been used extensively and intervention-oriented research is rare.

Research limitations/implications
Future research should to a larger extent address the interaction between the ISP management phases, apply more intervention research to develop computerised support for ISP management, investigate to what extent computerised support can enhance integration of ISP management phases and reduce the complexity of such a management process.

Practical implications
The limited focus on computerised support for ISP management affects the kind of advice and artefacts the research community can offer to practitioners.

Originality/value
Today, there are no literature reviews on to what extent computerised support the ISP management process. Findings on how the complexity of ISP management has been addressed and the research methods used extend beyond the existing knowledge base, allowing for a critical discussion of existing research and future research needs.
Evaluating graphical user interfaces for buildings
Authors: Halmetoja Esa , Forns-Samso Francisco 
Published: January 2020

Purpose
The purpose of this paper is to evaluate six different graphical user interfaces (GUIs) for facilities operations using human–machine interaction (HMI) theories.

Design/methodology/approach
The authors used a combined multi-functional method that includes a review of the theories behind HMI for GUIs as its first approach. Consequently, heuristic evaluations were conducted to identify usability problems in a professional context. Ultimately, thematic interviews were conducted with property managers and service staff to determine special needs for the interaction of humans and the built environment.

Findings
The heuristic evaluation revealed that not all the studied applications were complete when the study was done. The significant non-motivational factor was slowness, and a lighter application means the GUI is more comfortable and faster to use. The evaluators recommended not using actions that deviate from regular practice. Proper implementation of the GUI would make it easier and quicker to work on property maintenance and management. The thematic interviews concluded that the GUIs form an excellent solution that enables communication between the occupant, owner and service provider. Indoor conditions monitoring was seen as the most compelling use case for GUIs. Two-dimensional (2D) layouts are more demonstrative and faster than three-dimensional (3D) layouts for monitoring purposes.

Practical implications
The study provides an objective view of the strengths and weaknesses of specific types of GUI. So, it can help to select a suitable GUI for a particular environment. The 3D view is not seen as necessary for monitoring indoor conditions room by room or sending a service request. Many occupants’ services can be implemented without any particular layout. On the other hand, some advanced services were desired for the occupants, such as monitoring occupancy, making space reservations and people tracking. These aspects require a 2D layout at least. The building information model is seen as useful, especially when monitoring complex technical systems.

Originality/value
Earlier investigations have primarily concentrated on investigating human–computer interaction. The authors’ studied human–building interaction instead. The notable difference to previous efforts is that the authors considered the GUI as a medium with which to communicate with the built environment, and looked at its benefits for top-level processes, not for the user interface itself.
Bond–slip constitutive relation of mortar anchor under different loading rates
Authors: Wang Haitao , Guo Tao , Sun Haoyu 
Published: January 2020

Purpose
This paper aims to focus on establishing the bond-slip constitutive relation of mortar anchor under high loading rates by the dynamic pull-out test.

Design/methodology/approach
Self-made specimens were made for the dynamic pull-out test to explore the bond performance of mortar anchor, and the bond-slip constitutive relation of mortar anchor under high loading rates was established according to the analysis of test data.

Findings
During the loading process, the position of the peak bond stress was observed to translate to the free end. The bearing capacity of the mortar anchor was enhanced to some extent due to the increase of the loading rate.

Originality/value
The bond-slip constitutive relation of mortar anchor under high loading rates was established with the introduction of the position function and dynamic-load expanded coefficient.