bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic analysis reveals that Placenta-specific 9 induces cell proliferation and motility programs in human bronchial epithelial cells
Authors: Wang Hai-Xia , Qin Xu-Hui , Shen Jinhua , Liu Qing-Hua , Shi Yun-Bo , Xue Lu 
Published: July 2020

Abstract
Background: Abnormal reprogramming of airway epithelium is a key cause of pulmonary diseases. The molecular mechanism underlying the abnormal reprogramming of airway epithelial cells (AECs) remains to be elucidated. Placenta-specific protein 9 (Plac9), a putative secretory protein, initially identified in placenta, has previously been shown to affect cell proliferation and motility in human embryonic hepatic cells. Results: Interestingly, we found that Plac9 was repressed in lung cancers (LCs) compared to the corresponding normal tissues. We further investigated the role of Plac9 in human bronchial epithelial cells by constructing a stable Plac9-overexpressing cell line (16HBE-GFP-Plac9) and analyzing the effect of Plac9 on cellular protein composition by using an isobaric tag for relative and absolute quantification (iTRAQ) proteomic approach. By gene ontology (GO) and pathway analyses, we found that GO terms and pathways associated with cell proliferation, cell cycle progression, and cell motility/migration were significantly enriched among the proteins regulated by Plac9. Consistently, we observed that overexpression of Plac9 reduced cell proliferation and altered cell cycle progression. In addition, it also increased cell motility, including migration and invasion. Conclusions: Our findings suggest that Plac9 inhibits cell proliferation through S phase arrest by altering cyclins/cyclin-dependent kinases (CDKs) and promotes cell motility likely via the concerted actions of cyclins, E-cadherin and vimentin, which may underlie Plac9-mediated abnormal human bronchial pathogenesis.
Effect of Alpha-lipoic Acid Supplementation on Glycemic Control in the Patients with Metabolic Syndrome: A Randomized Clinical Trial
Authors: Kaviyani Nafiseh , Keshavarz Seyed Ali , Abbasi Behnood 
Published: July 2020

Abstract
BackgroundMetabolic syndrome is a collection of metabolic disorders. It is an important risk factor for progression towards type 2 diabetes and coronary artery diseases. Alpha-lipoic acid also plays a role as a co-factor for multi-enzyme complexes catalyzing oxidative. Antioxidant properties of alpha-lipoic acid (ALA) are associated with insulin-like effects. The present study examined the effect of alpha-lipoic acid supplementation on glycemic control in the patients with metabolic syndrome.MethodsA total of 46 patients with metabolic syndrome have participated in this double-blind randomized parallel-group clinical trial, 23 patients in one group received 600 mg of alpha-lipoic acid supplement and 23 patients in another group received the placebo for 12 weeks. RCT protocol was registered at ClinicalTrials.gov and it is available using NCT03589690 code. Fasting blood sugar (FBS), Serum insulin, Hemoglobin A1c (HbA1c), insulin resistance, were measured at the beginning and the end of the study. Dietary intake of participants was examined at the beginning of the study and this information was evaluated using Nutritionist 4 (N4) software. Studied information was analyzed by SPSS-24 software.ResultsThere were significant decreases in FBS levels (P = 0.009), serum insulin (P = 0.02), and insulin resistance (P = 0.001) between the two groups. Also, the HbA1c levels (P = 0.16) had no significant difference between the two groups.ConclusionALA is a potent antioxidant that it might be able to improve the blood glucose levels, serum insulin and insulin resistance; thus it will have positive effects on promoting the health levels in the patients with metabolic syndrome.Trial registrationNCT03589690
Core intestinal microbiomes of planktivorous and algae-farming coral reef damselfishes (Actinopterygii: Pomacentridae) reflects feeding behaviour
Authors: Kavazos Christopher Ronald James , Leggat William , Casey Jordan , Choat John Howard , Ainsworth Tracy D. 
Published: July 2020

Abstract
Background: Fish harbour diverse microbiomes within their gastro-intestinal system that effect the host’s digestion, nutrition and immunity. Despite the great taxonomic diversity of fish, little is understood about fish microbiome diversity and the factors that determine its structure and composition. Damselfish are important coral reef fish species that play a strong role in determining algae and coral structure of reefs. Broadly, damselfish belong to either of two trophic guilds based on whether they are planktivorous or algae-farming. In this study, we use 16s rRNA sequencing to interrogate the intestinal microbiome of 10 damselfish species (Pomacentridae) from the Great Barrier Reef to compare the composition of their intestinal bacterial assemblages across the planktivorous and algae-farming trophic guilds.Results: We identify core intestinal bacterial taxa for each host fish species. Gammaproteobacteria, belonging to the genus Actinobacillus, were detected in 80 % of sampled individuals and suggests a possible core member of pomacentrid microbiomes. Core microbiomes of algae-farming species were more diverse than planktivorous species with farming species sharing 35 ± 22 ASVs and planktivorous sharing 7 ± 3 ASVs. We also provide evidence for significant shifts in bacterial community composition along the intestines. We show that Bacteroidia, Clostridia and Mollicutes bacteria are more abundant in the anterior intestinal regions while Gammaproteobacteria are generally highest in the stomach. Finally, we highlight differences in microbiomes associated with both trophic guilds. Algae-farming and planktivorous damselfish host species significantly differed in their composition of bacteria belonging to Vibrionaceae, Lachnospiraceae and Pasteurellaceae.Conclusions: Our results demonstrate that core intestinal bacterial communities of damselfish reflect host species diet and feeding behaviour, whereby algae-farming hosts have larger and more diverse core microbiomes than planktivorous hosts. We suggest that the trophic guild of a host fish species is a strong determinant of microbiome structure.
Rapid Screening Diagnosis of SARS-COV-2 Infection With IgM-igG Combined Antibody Test Using Peripheral Blood
Authors: Li Zhengtu , Li Shaoqiang , Wang Youwei , Liao Yongkang , Chen Hui , Cheng Jing , Lin Ye , Chen Zhaoming , Sun Kangjun , Zhang Min , Wang Mindie , Wang Xinni , Yang Xinyan , Cai Wensheng , Zhan Yangqing , Li Shiyue , Zhong Nanshan , Ye Feng 
Published: July 2020

Abstract
Background

Rapid and convenient screening for identification of SARS-CoV-2 infected individuals are key to prevent and control this pandemic.
Methods

The peripheral blood samples were collected from coronavirus disease 2019 (COVID-19) patients and asymptomatic carriers to evaluate the test characteristics of the IgM-IgG combined assay for SARS-CoV-2 compared to that of serum samples and enzyme-linked immuno sorbent assay (ELISA). Close contacts, healthcare workers and workforces were recruited and screened using this assay.
Results

The sensitivity of the rapid IgM-IgG combined antibody test for SARS-CoV-2 using peripheral blood (sued as a POCT) was 97.0% and the specificity was 99.2%, which was consistent with the result obtained using serum sample (consistency is about 100%). Furthermore, this POCT assay also can detect IgM and IgG antibodies of SARS-CoV‐2 in asymptomatic carriers, with 19 of the 20 RT-PCR confirmed asymptomatic carriers testing positive. Therefore, this POCT assay was used for population screening of SARS-CoV-2 infection diagnosis. First, it found 4 positive close contacts among the 10 cases, and there were three IgM positive cases and one IgG positive case among them. It is worth noting that the IgM positive cases also tested positive for the nucleic acid of the SARS-CoV-2. Second, there was one IgM positive assay among the 63 healthcare workers, but RT-PCR of SARS CoV-2 was negative. Third, for workforces screening, there were no positive cases.
Conclusions

The IgM-IgG combined antibody test of SARS-CoV-2 can be used as a POCT for rapid screening of SARS-CoV-2 infection.
A Self-Selected Movement Classification Method for Forearm Via sEMG and Attitude Sensor
Authors: Zhang Lei 
Published: July 2020

Abstract
Objective: The use of surface electromyography (sEMG) to realize the recognition of the movement intention can realize the control of the artificial hand or the robot, and can help the rehabilitation training for hemiplegia or muscle weakness. However, the sEMG are weak and susceptible to external interference, so the current research focuses on identifying certain types of movements. But once the subjects are changed, the recognition accuracy will greatly reduce. This study proposes a classification method which the subject could choose optional movements of forearm.Methods: Two sEMG sensors were used, and a 9-axis attitude sensor was added to the wrist. 8 different subjects participated in the experiment, and everyone selected 5 movements. The sEMG sensors were attached to the extensor pollicis brevis and the extensor digitorum. The sEMG features were: Standard Deviation (SD), Power Spectrum Density (PSD); attitude sensor features were: angle and angular acceleration in three dimensional space, and integral of angular acceleration. The results were classified and identified using Linear Discriminant Analysis (LDA), K-Nearest Neighbor (KNN), Decision Tree (DT) and Ensembles (En) algorithms. The results of using the sEMG, using the attitude sensor signals and combining the two were compared. Analysis of variance was conducted on the average accuracy. Features were reduced the dimension by the Principal Component Analysis (PCA), and the results of using PCA and not were compared. Results: The results showed that the combination of the two types of sensors could improve the recognition effect compared to the using sEMG sensor or the attitude sensor alone. The final recognition result was that KNN performed best, reaching 95.0%. The results of using PCA were more stable.Conclusion: The method could be used between different subjects, and the user could select the movements autonomously.Significance: This method can improve the adaptability of movement intention recognition based on sEMG, and has important significance for popularizing the use of the sEMG to control the manipulator or the prosthetic and the rehabilitation training.
Documentation Practice and Associated Factors among Nurses in Harari Regional State and Dire Dawa Administration Governmental Hospitals, Eastern Ethiopia
Authors: Melkie Takla Tamir 
Published: July 2020

Abstract
Background: Nursing documentation is an integral and vital professional nursing practice, deals the process of recording nursing activities concerned with the care given to individual clients to assure continual effective, safe, qualified, evidence-based and individualized care. Despite of this, in Ethiopia there is a paucity of information on nursing documentation practice and its associated factors due to limited studies with methodological limitations. So this study aimed to assess documentation practice and identify its associated factors among nurses in six Governmental Hospitals of Harari Regional State and Dire Dawa Administration, Eastern Ethiopia. Methods: Institutional based cross-sectional study was conducted among 430 nurses and 421 medical records. Simple random sampling was employed for the selection of nurses and charts after the total sample size had allocated proportionally for each hospital. Data were collected by using self-administered questionnaire and review of records; entered and analyzed by using Epi data version 3.1 and statistical package for social sciences version 20.0 respectively. Logistic regression was used to identify the associated factors. Result: In this study, 47.5% of nurses were found to have good nursing documentation practice whereas good nursing documentation was found to practiced on 38.5% of medical records. Age, attitude, in-service training, nurse to patient ratio, availability of motivation and familiarity with standards of nursing documentation were found to had statistically significant positive association with documentation practice with AOR and 95%CI of 3.54 (1.170-10.8), 5.66 (3.17- 10.11), 2.53(1.477-4.35), 2.24(1.24-4.047), 4.60 (2.721-7.76) and 1.98 (1.137-3.44) respectively. Conclusion: Documentation was practiced poorly due to the identified factors. Therefore, it is better to put further effort towards improving documentation practice through providing training on standards of documentation and enhancing favorable attitude of nurses towards documentation by motivating them for their documentation activities.
Leveraging IgG N-glycosylation quantitative-trait loci to characterize the relationship between Type 2 Diabetes and hypertension: A network with bidirectional mendelian randomization study
Authors: Liu Di , Zhang Jie , Zhang Xiaoyu , Tian Qiuyue , Meng Xiaoni , Wu Lijuan , Song Manshu , Hou Haifeng , Wang Wei , Meng Qun , Wang Youxin 
Published: July 2020

Abstract
Background: The relationship between IgG N-glycosylation, type 2 diabetes (T2D) and hypertension is not well understood.Methods: A genome-wide association study (GWAS) of IgG N-glycosylation traits from 536 individuals was performed and 1203 IgG N-glycan quantitative trait loci (IgG N-glycan-QTL) variants targeting 24 IgG N-glycosylation were mapped traits after multi-testing correction. Network with bidirectional mendelian randomization (MR) analysis was performed to examine the causal association between IgG N-glycosylation, T2D and hypertension.Results: By linking IgG N-glycan-QTL variants with GWAS results for T2D and hypertension, 19 putatively causal IgG N-glycans for T2D and 21 putatively causal IgG N-glycans for hypertension were identified. IgG N-glycan-QTL determined IgG N-glycosylation to higher T2D was associated with higher hypertension risk (β [95% CI] =1.234 [0.939-1.529], P <0.001). In addition, IgG N-glycan-QTL determined IgG N-glycosylation to higher hypertension was associated with higher T2D risk (β [95% CI] =0.753 [0.140-1.3669], P =0.016). No evidence of pleiotropic bias was detected in MR-Egger analysis.Conclusions: Overall, our study showed that IgG N-Glycosylation-QTLs determined T2D is associated with higher hypertension risk, and vice versa, performing bidirectional regulation through IgG N-Glycosylation.
Metformin extends the lifespan of iMSUD mice by rescuing physiologic dysfunction and modulating mechanisms of mTORC1 activation
Authors: Hsu Yuehmei , Davis Sonnet , Liao Chen-Yu , Larson Samuel , Nava Vanessa , Wang Junying , Miller Delana , Kennedy Brian , Ramanathan Arvind 
Published: July 2020

Abstract
Background- Dysregulation of branched chain amino acid (BCAA) catabolism caused by mutations in the metabolizing enzyme-branched chain alpha-keto acid dehydrogenase (BCKDH) leads to the fatal newborn disorder Maple Syrup Urine Disease (MSUD) and is linked to chronic diseases such as type 2 diabetes. Results- Here we show that a MSUD mouse model with severely reduced BCKDH activity exhibits glucose intolerance, altered mTOR signaling, decreased levels of TCA cycle intermediates, reduced distribution of Type I skeletal muscle fibers and activated AMP-activated protein kinase (AMPK) signaling, all of which can be ameliorated by long term treatment with metformin, which enhances the survival of these mice. Treatment with metformin was correlated with increased serum-levels of growth/differentiation factor GDF15 (GFD15) and adiponectin. We also found that branched chain keto acid regulates leucine-mediated activation of mTORC1 by preventing the dissociation of sestrin2-gator complex in vitro. Conclusions- In summary, our study suggests metformin could be an effective therapeutic candidate for MSUD patients and maps the crosstalk between key pathways that maintain metabolic homeostasis in MSUD.
Roxadustat (FG4592) alleviates Radiation-Induced Intestinal Injury by facilitating recovery of intestinal stem cell and reducing damage of intestinal epithelial cell
Authors: Xia Penglin , Yang Yajie , Liu Ruling , Tang Haibo , Du Jicong , Cheng Ying , Cai Jianming , Liu Cong , Xu Xiaohua , Liu Hu 
Published: July 2020

Abstract
Background: Radiation induced-intestinal injury (RIII) is a common complication after radiation therapy in patients with pelvic, abdominal or retroperitoneal tumors. The mechanism of RIII includes radiation-induced death of intestinal epithelial cells (IECs) and damage of intestinal stem cells (ISCs), among which damage of ISCs is main cause. Most recently, hypoxia Inducible factor (HIF) has been found to have effect on maintaining stemness and promoting proliferation of ISCs, which suggests a protective role of HIF in the RIII. In this study, we investigated the effect of FG4592, a novel up-regulator of HIF, on the protection of RIII, and further researched its function on ISCs.Methods: With/without FG4592 treatment, the abdomen of mice was radiated at dose of 25Gy, and then the degree of intestinal injury was assessed by H&E staining and Brdu label. By intestinal organoid culture, the multiplication capacity and differentiation features of ISCs were detected. Besides, the effects of FG4592 on the radiated IECs were also evaluated by detecting cell viability, apoptosis, and proliferation potential.Results: FG4592 could effectively up-regulate the expression of HIF-1α and HIF-2α in vivo. An abdominal radiation of 25Gy established the RIII model of mice, by which FG4592 was verified to have protective effect on the intestine from radiation. Morphology and Brdu test of intestinal organoid showed that FG4592 could promote regeneration and differentiation in ISCs after radiation, which were mediated by up-regulating HIF-2 rather than HIF-1.Conclusion: FG4592, a novel up-regulator of HIF could remit RIII and promote regeneration and differentiation of ISCs after radiation, which were depend on HIF-2 rather than HIF-1.
Mitigating Implicit Bias in Patient-Clinician Communication in Clinical Encounters: Early Insights from the COmmuNity-engaged SimULation Training (CONSULT) Trial
Authors: Tjia Jennifer , Pugnaire Michele , Calista Joanne , Esparza Nancy , Valdman Olga , Garcia Maria , Yazdani Majid , Hale Janet , Terrien Jill , Eisdorfer Ethan , Zolezzi-Wyndham Valerie , Chiriboga Germán , Rappaport Lynley , Puerto Geraldine , Dykhouse Elizabeth , Potts Stacy , Sifuentes Andriana Foiles , Stanhope Sylvia , Allison Jeroan , Duodu Vennesa , Sabin Janice 
Published: July 2020

Abstract
BackgroundTo address the gap in knowledge about how to design feasible and acceptable trainings for clinicians that aim to mitigate implicit bias in clinical encounters, we report the early insights from the COmmuNity-engaged SimULation Training for Blood Pressure Control (CONSULT) Trial.MethodsWe engaged academic and community stakeholders to design, pilot test and implement a training program addressing healthcare disparities knowledge, bias awareness, and communication skills focused on bias mitigation. A stepped wedge cluster randomized trial was developed to determine intervention dose effects. We assessed the CONSULT training program through structured feedback using online surveys, real-time comments, and individualized feedback from trainees, faculty and standardized patients.ResultsThe first training cohort completed the intervention (N = 64). Feedback prompted training program revisions as follows: reducing overall time burden and the number of implicit bias assessments; supplementing on-line learning with augmented in-person interactive sessions. Feedback also reinforced the critical importance of having highly skilled facilitators versed in implicit bias.ConclusionsIterative stakeholder engagement is essential for developing and revising educational interventions aimed at raising bias awareness and mitigating the effects of implicit bias.Trial RegistrationClinicalTrials.gov, NCT 03375918. Registered December 18, 2017, https://clinicaltrials.gov/ct2/show/NCT03375918?id=NCT+03375918&draw=2&rank=1
Cellular Compatibility of a New Tantalum-Niobium Scaffold Material
Authors: Ouyang Jianan , Deng Zhenhan , Chen Kang , Xiong Jianyi , Li Ying , Wang Daping , Lu Wei , Zeng Yanjun , Zhu Weimin 
Published: July 2020

Abstract
[Objective] To determine the cellular compatibility of porous tantalum-niobium (Ta-Nb) material. [Method] Rabbit osteoblasts were co-cultured with porous Ta-Nb material. The cell proliferation was detected by CCK-8 method, and the cell adhesion was observed under scanning electron microscope (SEM). The expressions of type-I collagen and osteocalcin were detected by RT-PCR assay. [Results] CCK-8 detection indicated that the cell proliferation on the porous Ta-Nb material showed no difference from that of the control group (P>0.05). SEM revealed that a large amount of cells adhered onto the surface and in the pores of the material. The number of cells on the material surface increased obviously over time. RT-PCR assay showed that with the prolonging of the time of co-culture, the expression of type-I collagen was enhanced (P<0.05), while the osteocalcin expression exhibited no significant difference (P>0.05[Conclusion] Porous Ta-Nb scaffold material can be used to promote the adhesion, growth and differentiation of osteoblasts with satisfactory cellular compatibility.
Comparison Between the Nature and Activity of the Silver Nano Particles on Cervical Cancer Cells That Were Produced by the Active and Inactive Fungal Biomass Forms
Authors: Pourali Parastoo , Nouri Mahnaz , Heidari Tana , Kheirkhahan Niloufar , Yahyaei Behrooz 
Published: July 2020

Abstract
Background Biologic nanoparticles can be produced by the active and passive mechanisms but their properties have not been compared to each other and recent research tries to uncover them.Methods Biomass of Fusarium oxysporum was divided into four groups and pretreated in: room temperature (RT) and refrigerator (for preparation the active biomass forms); autoclave and hot air oven instruments (for preparation the inactive biomass forms). Samples were floated in ddH2O and SNPs were produced after addition of 1mmol final concentration of silver nitrate solution. Production of SNPs was confirmed using visible spectrophotometer, transmission electron microscopy (TEM) and X-ray diffraction (XRD) analyses. SNPs were washed and their concentration was determined using ICP measurement. Plate well diffusion method was used for antibacterial activity. MTT and Annexin V-FITC/ propidium iodide assays were used for cytotoxicity and apoptosis induction assays, respectively. Result Results showed that all the four tested samples produced SNPs and had maximum absorbance peaks about 400-450 nm. The produced SNPs had almost same shapes and sizes and XRD results confirmed the presence of SNPs in all the samples. ICP results showed that except the refrigerator incubated sample, the concentration of the SNPs in all the flasks was similar to each other. Due to the differences in the nature of the applied bacterial strains the produced SNPs had some differences in their antibacterial activities. MTT assay results showed that due to lower SNPs concentration of the refrigerator incubated sample they had less toxic effects than the others. Apoptosis induction results revealed that the biologically produced SNPs induced more apoptosis and less necrosis.Conclusion In conclusion, the active and inactive fungal biomass forms produced SNPs that their nature and activity were not altered.