bluuebunny/crossref_metadata_2025_split huggingface dataset:
Randomized controlled trial on the effectiveness of web‐based Genomics Nursing Education Intervention for undergraduate nursing students: a study protocol
Authors: Dumo Anndra Margareth , Laing Bobbi , Lim Anecita Gigi , Palaganas Erlinda , Abad Peter James , Valdehueza Ofelia , Palovaara Marjo , Saunders Hannele , Estola Matti , Mandysova Petra , Maguire Jane , Ward Linda D. , Carlberg Carsten , Vehviläinen‐Julkunen Katri 
Published: August 2020

AbstractAimTo describe a randomized controlled trial protocol that will evaluate the effectiveness of two web‐based genomic nursing education interventions.BackgroundPreparing future nurses to be competent in genetic and genomic concepts is fundamental to ensure appropriate clinical application. However, genetics–genomics concepts are still new in the field of nursing. Little is known about what type and kind of web‐based nursing education is effective in improving the knowledge of nursing students. To address these knowledge gaps, a web‐based ‘Genomic Nursing Education Intervention’ will be developed and compared with an existing online education programme.DesignA randomized controlled trial of two groups with pre‐test and repeated posttesting.MethodsThe Genomic Nursing Concept Inventory, a validated tool, will be used to assess the genetics–genomics knowledge of nursing students. Participants will be randomly allocated to either a control or an intervention group. The control group will receive the standard web‐based nursing education, while the intervention group will receive a newly developed web‐based education intervention. Outcome measures include the students’ knowledge level of nursing genetics–genomics concepts. Participants will be retested at 3 and 6 months.ConclusionCurrent evidence shows that ensuring nurses have adequate education in genetic–genomic concepts is challenging. This study will demonstrate which of two web‐based nursing education methods is more effective in teaching genetic–genomic concepts. This research project will better prepare the nursing profession in their careers for the emerging advance technologies in genetics–genomics and personalized health care.ImpactCurrent evidence shows major challenges in ensuring that nurses have adequate education in genetics–genomics concepts. Less is known about what approaches to web‐based education are effective to improve the knowledge gaps of nursing students in genetics–genomics concepts. This study will determine which type of web‐based nursing education is effective in improving the genetics–genomics knowledge of nursing students. This research project will help better prepare nurses in dealing with advances in genetics–genomics in their careers.Trial registration: This study is registered in ClinicalTrials.gov (ID number NCT03963687) https://clinicaltrials.gov/show/NCT03963687
Describing self‐care and its associated variables in ostomy patients
Authors: Giordano Vittoria , Nicolotti Matilde , Corvese Francesco , Vellone Ercole , Alvaro Rosaria , Villa Giulia 
Published: August 2020

AbstractAimsTo describe self‐care in ostomy patients, to identify socio‐demographic and clinical variables associated with self‐care and to identify the association between self‐care self‐efficacy and self‐care over and above the variables associated with self‐care.DesignLongitudinal and multicentre study. Data were collected between February 2017‐May 2018.MethodsIn this study, 523 ostomy patients were enrolled at baseline (T0) and 362 were followed‐up after 6 months (T1). The Ostomy Self‐Care Index was used to measure self‐care maintenance, monitoring, management, and self‐efficacy. Correlations between self‐care dimensions and patient socio‐demographic and clinical characteristics were performed with Pearson's or Spearman's correlations. Three separate two‐step hierarchical regression analyses were performed to identify variables associated with self‐care maintenance, monitoring, and management.ResultsParticipants’ mean age was 69 years (SD 12.4); 63.9% were male and most had enterostomies (38.8% colostomies, 29.3% ileostomies) and permanent ostomies (72.5%). Patients had adequate self‐care maintenance and monitoring at T0 and T1, while they had lower self‐care management and self‐efficacy at baseline. Significant variables associated with better self‐care maintenance and self‐care monitoring were female gender, more information received during hospitalization and better autonomy in stoma management, while a better level of education was an additional variable associated with self‐care monitoring. Self‐care self‐efficacy produced a significant increase in the explained variance of self‐care maintenance and self‐care monitoring. None of the selected variables were significantly associated with self‐care management.ConclusionMiddle‐high levels of self‐care maintenance, monitoring, management, and self‐efficacy were found. The variables associated with ostomy self‐care and the role of self‐care self‐efficacy identified in this study can help in developing tailored nursing interventions.ImpactThis study found specific variables associated with ostomy self‐care which could contribute to guiding future interventions aimed at improving self‐care in ostomy patients.
Identification of microRNA associated with the elimination of hepatitis C virus genotype 1b by direct‐acting antiviral therapies
Authors: Yoneyama Hirohito , Morishita Asahiro , Iwama Hisakazu , Fujita Koji , Masaki Takahiro , Tani Joji , Tadokoro Tomoko , Nomura Takako , Sakamoto Teppei , Oura Kyoko , Takuma Kei , Nakahara Mai , Mimura Shima , Deguchi Akihiro , Oryu Makoto , Tsutsui Kunihiko , Himoto Takashi , Shimotohno Kunitada , Wakita Takaji , Kobara Hideki , Masaki Tsutomu 
Published: August 2020

AbstractBackground and AimDirect‐acting antiviral (DAA) therapies have been proven to be highly effective for the eradication of hepatitis C virus (HCV) without resistance‐associated substitutions (RASs). However, even in cases with no detected RASs, treatment sometimes fails, suggestive of the existence of some host‐related factors involved in HCV eradication by DAAs. To explore such factors, we analyzed the serum microRNAs (miRNAs) of patients who received DAA treatment.MethodsThe serum miRNA expression levels of 39 patients with chronic HCV infection without any detectable RASs, who achieved sustained virological response with asunaprevir/daclatasvir or grazoprevir/elbasvir therapy, were investigated cyclopedically, using oligonucleotide microarrays. The effects of specific miRNAs on the replication of HCV were measured in the HCV genomic replicon containing Huh‐7 hepatoma cells.ResultsAlong with the disappearance of HCV, the expression quantiles of 16 miRNAs in the asunaprevir/daclatasvir group and 18 miRNAs in the grazoprevir/elbasvir group showed a tendency to increase or decrease. Among these molecules, adjustments for multiple testing yielded a significant differential expression at a false discovery rate of less than 5% for only one molecule, hsa‐miR‐762. Its expression quantile increased after HCV exclusion in all patients who had achieved sustained virological response. Quantitative polymerase chain reaction analysis validated a significant increase in the serum hsa‐miR‐762 after disappearance of HCV. On the contrary, hsa‐miR‐762 was decreased in the relapse and breakthrough of HCV in DAA failures. Transfection of hsa‐miR‐762 into cultured HCV‐infected hepatocytes significantly decreased HCV‐RNA replication.ConclusionThese data suggest that hsa‐miR‐762 is one of the host factors participating in HCV exclusion by DAA therapy.
Accessory bones of the elbow: Prevalence, localization and modified classification
Authors: Kunc Vojtěch , Kunc Vladimír , Černý Vojtěch , Polovinčák Michal , Kachlík David 
Published: August 2020

AbstractAccessory bones in the region of the elbow are rare variants with high clinical significance as they may be confused with avulsion fractures. We investigated their prevalence and performed a statistical analysis to support their congenital origin. Their localization was mapped to show their exact site of occurrence. We evaluated anteroposterior and lateral X‐ray images of 2413 elbows in a Central European population from which a group of accessory bony structures was selected. Their character was evaluated, and accessory bones were identified. We used logistic regression to evaluate the potential relationship between the occurrence of accessory bones, the age of patients, and the occurrence of calcar olecrani. The prevalence of accessory bones of the elbow in the sample was 0.77%. Our results did not show a statistically significant relationship with the occurrence of calcar olecrani or with the age of patients. The most common type was os subepicondylare mediale (type V) in 0.46%, which was located distal to the medial epicondyle of the humerus, followed by os subepicondylare laterale (type III; 0.21%), situated laterally to the lateral epicondyle of the humerus. In comparison with previous reports, os sesamoideum mediale (type IV) was located more distally. Our data suggest that congenital accessory bones are a rare entity. Knowledge of their exact localization should be considered during diagnosis of avulsion fractures and other unclear diagnoses including accessory bony structures in the elbow region.
Effect ofepigallocatechin‐3‐gallateon the status ofDNAmethylation of E‐cadherin promoter region on endometriosis mouse
Authors: Guan Qi‐Hui , Shi Wen‐Jing , Zhou Long‐Shu , Tao Ai‐Lin , Li Lu 
Published: August 2020

AbstractAimTo evaluate whether epigallocatechin‐3‐gallate acts on endometriosis mouse, and changes the status of DNA methylation of E‐cadherin promoter region.MethodsAccording to our previous research, the tracing nude mouse model of endometriosis was built up and randomly divided into three groups: control group (group A), epigallocatechin‐3‐gallate group (group B) and decitabine group (group C). Normal saline, epigallocatechin‐3‐gallate and decitabine were isometrically intraperitoneally injected into each group once in 2 days. In this period, the growth situations of lesions were monitored by living image system. After 16 days, the lesions were taken out and the distribution of E‐cadherin and its methylated situation of promoter region were analyzed.ResultsThe region of interest of ectopic lesion increased from 4th to 16th day in group A (P < 0.01); in group B and C, the region of interest of ectopic lesion increased in the 0–8th day (P < 0.01), and decreased in the 8–16th day (P < 0.01). The positive expression rate of E‐cadherin in group C was higher than group B, and group B was higher than group A (P < 0.01). The DNA methylation status of E‐cadherin promoter region in group A was higher than group B, and group B was higher than group C (P < 0.01).ConclusionEpigallocatechin‐3‐gallate may inhibit the growth of endometrial lesion, affect the expression of E‐cadherin on the cell membrane and reduce the status of DNA methylation of E‐cadherin promoter region.
Establishment and evaluation of a risk‐scoring system for lymph node metastasis in early‐stage endometrial carcinoma: Achieving preoperative risk stratification
Authors: Zhang Ying , Zhao Weidong , Chen Zhengzheng , Zhao Xuxu , Ren Pingping , Zhu Meiling 
Published: August 2020

AbstractAimTo establish a risk‐scoring system for lymph node metastasis (LNM) of early‐stage endometrial carcinoma (EC), and to stratify the preoperative risk of LNM.MethodsWe retrospectively analyzed the clinical data of 507 patients diagnosed with the early‐stage EC (i.e., confined to the uterine corpus). We determined the risk factors for LNM by logistic regression analysis; then constructed a simple logistic scoring system, and an additive scoring system based on the regression coefficient (β), and odds ratio, of each variable, respectively.ResultsThe overall rate of LNM was 9.1% (46/507). Multivariate analysis showed that preoperative serum cancer antigen 125 (CA125) ≥35 U/mL, histopathology of grade 3 and/or type II, depth of myometrial invasion ≥1/2 and positive immunostaining for Ki‐67 ≥50%, were independent risk factors for LNM (P < 0.05). The simple logistic and additive scoring systems exhibited good predictive ability (area under the curve [AUC] >0.8). Based on the additive scoring system, the risk of LNM in patients with early‐stage EC was classified into three groups: a low‐risk group (total score: <5), an intermediate‐risk group (total score: 5–10) and a high‐risk group (total score: >10). The incidence of LNM differed significantly across these three groups (P < 0.05).ConclusionThe risk‐scoring system constructed in this study can effectively predict the risk of LNM in patients with early‐stage EC, achieve preoperative risk stratification and provide a reference guideline for the use of lymphadenectomy.
Randomized controlled trial of oral bicarbonate treatment for labor stagnation
Authors: Seyedi Mitra , Ghorashi Zohreh , Sedighi Darijani Tayebeh 
Published: August 2020

AbstractAimThe aim of this study was to determine the effect of oral sodium bicarbonate solution on delivery outcome of primiparous women with labor stagnation.MethodsThis randomized controlled trial study was performed on 142 primiparous pregnant women referred to Jiroft University hospitals who had stagnation during labor. In the intervention group, in addition to oxytocin, 4.26 g of sodium bicarbonate dissolved in 200 mL of water were given orally. The control group received only oxytocin. Data were analyzed by t‐test and chi‐square test.ResultsThe mean interval of dilatation stopping to delivery in the intervention group was significantly lower than the control group (202.34 vs 304.08 min, P = 0.001). The mean duration of the first and second stages of labor in the intervention group was lower than the control group (280.44 vs 397.16 and 35.00 vs 51.72 min respectively, P = 0.001). There was no statistically significant difference between the intervention and control groups in the rate of cesarean section and Apgar score. In the intervention group, the rate of spontaneous delivery was significantly higher (65 vs 56 cases, P = 0.03) than the control group.ConclusionThe results of the present study could suggest the use of oral bicarbonate in stagnation as a cheap and no‐cost method to facilitate normal vaginal delivery.
RNA in situ hybridization for human papillomavirus testing in oropharyngeal squamous cell carcinoma on a routine clinical diagnostic platform
Authors: Henley‐Smith Rhonda , Santambrogio Alice , Andoniadou Cynthia L. , Odell Edward , Thavaraj Selvam 
Published: August 2020

BackgroundThe current diagnostic standard for detection of high‐risk human papillomavirus (HPV) in oropharyngeal squamous cell carcinoma is via a two‐stage algorithm, namely p16 immunohistochemistry followed by HPV DNA in situ hybridization in p16 positive cases. This study evaluated the feasibility of automated RNA in situ hybridization on a clinical platform as a single‐step alternative to the two‐stage algorithm within a routine diagnostic histopathology setting.MethodsThirty‐eight cases positive for both p16 and DNA in situ hybridization, 42 p16 negative cases and 20 cases positive for p16 but negative for DNA in situ hybridization were randomly selected. High‐risk HPV RNA in situ hybridization was undertaken on all cases on an automated clinical platform. Manufacturer‐recommended and on‐slide additional p16/HPV positive and negative controls were used. Test quality assurance and diagnostic RNA in situ hybridization were independently assessed by two observers. A consensus diagnosis was reached in the presence of a third observer on discordant cases. All RNA in situ hybridization results were then correlated against p16 and DNA ISH status.ResultsInter‐slide RNA in situ hybridization staining variation was observed in control sections. RNA in situ hybridization demonstrated a high inter‐observer agreement rate (κ = .897, P < .001). Following consensus review, there was full concordance between RNA in situ hybridization and the current standard.ConclusionHuman papillomavirus testing by standalone automated RNA in situ hybridization on a clinical diagnostic platform currently available in routine diagnostic histopathology laboratories is a feasible alternative to the two‐step algorithm of p16 and DNA in situ hybridization. Control tissue staining procedures need to be adapted to achieve the most accurate results.
Low potassium diets with different levels of calcium in comparison with different anionic diets fed to prepartum dairy cows: Effects on sorting behaviour, total tract digestibility, energy metabolism, oxidative status and hormonal response
Authors: Rajaeerad Abbas , Ghorbani Gholam R. , Khorvash Mohammad , Sadeghi‐Sefidmazgi Ali , Mahdavi Amir H. , Wilkens Mirja R. 
Published: August 2020

AbstractThis study investigated the effects of low potassium diets with different levels of Ca compared to two diets low in dietary cation–anion difference (DCAD) fed prepartum as a strategy to prevent hypocalcemia on sorting behaviour, total tract digestibility, oxidative status and energy and protein metabolism of transition cows. Forty‐eight pregnant dairy cows were assigned to 4 treatment groups: Low Ca, low K (LCLK), High Ca, low K (HCLK), Supplementation with anionic mineral mixture (AMS) supplementation with SoyChlor (CAS). After parturition, all animals were fed a standard postpartum diet. Data were collected until 21 DIM. Prepartum urinary pH was significantly reduced by the low DCAD diets, while postpartum Ca homeostasis was affected by the HCLK ration. Feeding AMS induced sorting against particles <1.18 mm in favour of particles >19 mm prepartum. In contrast, cows fed CAS showed an increase in selective consumption of fine particles and sorted against longer particles similar to the HCLK and LCLK groups. Postpartum sorting activity was not affected by the dietary treatments. After calving, apparent digestibility of NDF was significantly reduced in the HCLK group. Prepartum, we observed effects on serum concentrations of non‐esterified fatty acids were higher and insulin sensitivity was lower in the AMS group. Blood urea nitrogen (BUN) was decreased in cows fed the CAS ration. Postpartum, we found serum protein to be decreased with the low DCAD diets while BUN was decreased in the CAS group. The low DCAD rations increased prepartum serum malondialdehyde concentrations, while postpartum total antioxidant capacity was lower in the HCLK and the AMS group. From these data, we conclude that AMS decreased prepartum intake due to compromised palatability. Intermediate protein metabolism was affected by the low DCAD diets, while parameters of oxidative stress were probably affected by acid–base balance and Ca homeostasis.
Psychometric properties of the Turkish version of the Diabetes Family Impact Scale
Authors: Cetintas Ismail , Akgün Kostak Melahat 
Published: August 2020

AbstractPurposeThe objective of this study was to examine the psychometric properties of the Turkish version of the Diabetes Family Impact Scale (DFIS).Methods and DesignThe study was carried out methodologically with 121 parents who have 6–18 year‐old children with type 1 diabetes (n = 121). The collected data were analyzed by conducting confirmatory factor analysis, t test, and Pearson's correlation analysis and by computing Cronbach's α and intraclass correlation coefficients (ICCs).ResultsThe linguistic validity of the scale was ensured by undertaking translation and back‐translation. Expert opinions were analyzed and all the content validity indices were found to be ≥0.80. The factor loadings of all the items ranged from 0.47 to 0.89, and the model fit indices were as follows: χ²/df = 1.21 and RMSEA = 0.04. Scores that were obtained across two temporally separated administrations were not significantly different (p > .05), and there was a strong correlation between the two sets of scores (ICC = 0.892; p < .001). The Cronbach's α of the scale was .881. The item‐total correlation coefficients of the scale items ranged from .34 to .72.ConclusionsSimilar to the original assessment, the Turkish version of the DFIS consists of 14 items and 4 subscales. It is a valid and reliable measure that is suitable for use with Turkish samples. Researchers can use this scale to assess the effect of type 1 diabetes mellitus on the family of an affected child.
Efficacy of various coated materials to prevent nutrient leaching for Pacific white shrimp Litopenaeus vannamei commercial diets
Authors: Guo Jingping , Davis Robert , Starkey Charles , Davis Donald Allen 
Published: August 2020

AbstractThe physical characteristics and leaching rates of water‐soluble compounds from shrimp feeds are major considerations in nutrient delivery. Yet, there is limited information on nutrient leaching from feeds or the mechanisms to minimize leaching. This study evaluated the efficacy of various coating agents on the physical characteristics and leaching of nutrients from commercial feeds for Litopenaeus vannamei. A series of diets were top coated with two lipid sources (fish or stearin oil) and two additives to the oil (5% wax or gelatin) at three levels (0, 1.5, and 3%). Physical characteristics and leaching of water‐soluble compounds were then characterized. The reduction of leaching was seen with the diets that were top coated with 1.5 or 3% oil, which significantly decreased the leaching of phenylalanine (Phe) after 30 min and 60 min of immersion. Adding 1.5 and 3% oil coating significantly decreased the pellet durability index. Adding gelatin as an additive to the oil significantly slowed sinking velocity and improved water stability and the pellet durability index of the diets. In all, results demonstrated that coating 1.5 or 3% fish or stearin oil decreases the leaching rate. Supplementing 5% gelatin in the coating material for diets can improve the performance of the diets.
Reducing malnutrition in Cambodia. A modeling exercise to prioritize multisectoral interventions
Authors: Laillou Arnaud , Gauthier Ludovic , Wieringa Frank , Berger Jacques , Chea Samnang , Poirot Etienne 
Published: August 2020

AbstractUndernutrition is a major contributor to child morbidity and mortality and poses a large burden to the economy, in Cambodia. This study explored factors contributing to child stunting and wasting and their regional inequalities among 1,938 Cambodian children aged 6–23.9 months. Data were drawn from a longitudinal study (year 2017) conducted in six districts of two north‐eastern provinces and the capital and used as cross‐sectional. Socio‐demographic and household characteristics, children's feeding practices during the previous 24 hr, and children's length and weight measurements were collected. Gradient boosting models were used to calculate the contribution of determinants to child undernutrition whereas concentration index was used to assess the impact of those determinants on stunting and wasting inequalities among socioeconomic groups. It was found that low‐household wealth could predict 21% to 45% of child stunting and 23% to 36% of wasting across regions. After wealth, source and treatment of drinking water were found the second major predictor for stunting (15%) and wasting (21%). Combining child nutrition and household water, sanitation and hygiene indicators predicted around 30% of child undernutrition, either in the form of stunting or wasting. Mothers' education predicted >30% of stunting in the north‐eastern region. Results highlight that a complex interplay of factors contributes to child stunting and wasting. An integrated, intersectoral, equity‐focused approach that addresses children's dietary quality, household's water, sanitation and hygiene conditions, mother's education, and poverty is likely to yield the highest impact in achieving further gains in nutritional status among Cambodian children.