bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peripheral blood inflammatory markers in predicting prognosis in patients with COVID‐19. Some differences with influenza A
Authors: Zhao Yan , Yu Chao , Ni Wei , Shen Hua , Qiu Mengqi , Zhao Youyun 
Published: November 2020

AbstractBackgroundTo evaluate the ability of peripheral blood inflammatory markers in predicating the typing of COVID‐19, prognosis, and some differences between COVID‐19 and influenza A patients.MethodsClinical data on 285 cases laboratory‐confirmed as SARS‐CoV‐2 infection were obtained from a Wuhan local hospital's electronic medical records according to previously designed standardized data collection forms. Additional 446 Influenza A outpatients’ hematologic data were enrolled for comparison.ResultsNLR, SII, RLR, PLR, HsCRP, and IL‐6 were significant higher and LMR was lower in severe COVID‐19 patients than in mild COVID‐19 patients (p < .001). PLR and LMR were lower in the individuals with influenza A than those with COVID‐19 (p < .01). COVID‐19 patients with higher levels of NLR, SII, RLR, PLR, HsCRP, and IL‐6 and lower LMR were significantly associated with the severe type. AUC of NLR (0.76) was larger while the specificity of IL‐6 (86%) and sensitivity of HsCRP (89%) were higher than other inflammatory markers in predicating the typing of COVID‐19. PT had obvious correlation with all the inflammatory markers except RPR. NLR showed positive correlations with AST, TP, BUN, CREA, PT, and D‐dimer. Patients with high IL‐6 levels have a relatively worse prognosis (HR = 2.30).ConclusionPeripheral blood inflammatory markers reflected the intensity of inflammation and associated with severity of COVID‐19.NLR was more useful to predict severity as well as IL‐6 to predict prognosis of COVID‐19. PLR and LMR were initially found to be higher in SARS‐CoV‐2 virus‐infected group than in influenza A.
Measurement of methotrexate in human cerebrospinal fluid using a chemiluminescence immunoassay intended for serum and plasma matrices
Authors: Yoshikawa Naoki , Yamada Ai , Yokota Tsubasa , Moritake Hiroshi , Hirabara Yasutoshi , Ikeda Ryuji 
Published: November 2020

AbstractBackgroundThe concentration of MTX in blood is often measured quickly and easily by immunoassays. Thus, immunoassays may facilitate the easy determination of the concentration of MTX in the cerebrospinal fluid (CSF). In this study, we measured methotrexate (MTX) concentrations in the CSF using a high‐performance liquid chromatography (HPLC) method intended for analyzing CSF matrices and a chemiluminescence immunoassay (CLIA) method intended for assessing serum and plasma matrices and verified the differences in the results of the two methods.MethodsHPLC analysis for MTX in the CSF was performed using a Prominence UFLC system with a C18 column. The HPLC method was validated in accordance with the 2018 FDA guideline. The CLIA method was performed using an ARCHITECT i1000SR system intended for serum and plasma matrices. A total of 47 CSF samples (14 clinical and 33 spiked specimens) were analyzed using the two methods.ResultsThe HPLC method passed the validation criteria. The concentration of MTX in the same sample, determined using the HPLC and CLIA methods, differed proportionally; the percent difference in the concentrations averaged −23.0% (95% confidence interval: −36.9% to −9.1%) as revealed by the Bland‐Altman plot. The relationship between the measured values, evaluated using the Passing‐Bablok regression, was as follows: HPLC = 1.205 × CLIA – 0.024.ConclusionThe equation deduced in this study can be used to correct the concentration of MTX measured using the CLIA method.
Best friends, bad food, and bullying: How students’ school perceptions relate to sense of school belonging
Authors: Renick Jennifer , M. Reich Stephanie 
Published: November 2020

AbstractA positive school climate and sense of school belonging can influence students' academic outcomes and wellbeing in desirable ways. However, not enough is known about the aspects of school climate that influence students' feelings of belonging and how gender, ethnicity, and grade may relate to those feelings. Via a self‐administered survey, a diverse sample of middle school students (n = 1,226) reported what they perceived as the best parts of their school and the parts most in need of improvement, as well as their sense of belonging. Students' perceptions of their school were aligned with the major areas of school climate: safety, relationships, teaching and learning, and institutional environment. These four areas were found to be predictive of sense of belonging to different degrees, such as listing relationships as needing improvement at the school being associated with lower scores in sense of belonging. Gender and grade were also found to be salient predictors of feelings of belonging, with seventh and eighth graders feeling less belonging than their sixth grade peers and girls having lower sense of belonging than boys. These findings affirm the importance of the school environment in influencing students' feelings of being a part of their school.
Dyadic report of relationship quality in school‐based mentoring: Effects on academic and behavioral outcomes
Authors: Jablon Elana M. , Lyons Michael D. 
Published: November 2020

AbstractMentoring, a common support practice for middle schoolers, can have varying effects on student academic and behavioral outcomes depending on the type of mentoring and quality of relationship formed. Although research has examined mentees' perception of relationship quality in mentoring relationships, fewer studies have looked at both mentor and mentee reports. The present study aims to explore how the interaction of mentor and mentee perceptions of the relationship quality is associated with student academic and behavioral outcomes. Major findings include the significant association between mentor‐reported relationship quality and academic outcomes for mentees, as well as some associations mentee‐ and mentor‐reported relationship quality and behavioral outcomes. Results illustrate the importance of training in mentoring programs, as well as how mentee‐ versus mentor‐report of the relationship may impact outcomes in distinct ways. This study can improve understanding of mentor–mentee relationships, which may improve student academic and behavioral outcomes.
Prevalence of and factors associated with advanced HIV disease among newly diagnosed people living with HIV in Guangdong Province, China
Authors: Jiang Hongbo , Liu Jun , Tan Zhimin , Fu Xiaobing , Xie Yingqian , Lin Kaihao , Yan Yao , Li Yan , Yang Yi 
Published: November 2020

AbstractIntroductionA high proportion of people living with HIV (PLHIV) present for care with advanced HIV disease (AHD), which is detrimental to “90‐90‐90” targets to end AIDS by 2030. This study aimed to explore the prevalence of and factors related to AHD among newly diagnosed PLHIV in Guangdong Province, China.MethodsNewly diagnosed PLHIV were recruited from six cities in Guangdong Province from May 2018 to June 2019. AHD was defined as an initial CD4 count <200 cells/µL or an AIDS‐defining event within one month of HIV diagnosis. Data from a questionnaire and the national HIV surveillance system were used to explore the potential factors related AHD.ResultsA total of 400 of 997 newly diagnosed PLHIV were defined as having AHD with a proportion of 40.1%. After adjusting for statistically significant variables in univariate analysis, multivariable logistic regressions showed that individuals aged 30 to 39 years (adjusted odds ratio (aOR) = 1.77, 95% confidence interval (CI): 1.13 to 2.79) and ≥50 years (aOR = 1.98, 95% CI: 1.15 to 3.43) were at a higher risk of AHD than those aged 18 to 29 years. Participants diagnosed by voluntary counselling and testing (VCT) clinics were less likely to have AHD (aOR = 0.67, 95% CI: 0.48 to 0.94) than those diagnosed at medical facilities. Participants who had ever considered HIV testing (aOR = 0.66, 95% CI: 0.45 to 0.98) and who had high social support (aOR = 0.73, 95% CI: 0.55 to 0.97) were at a lower risk of AHD, whereas participants who had HIV‐related symptoms within one year before diagnosis were at a higher risk of AHD (aOR = 2.09, 95% CI: 1.58 to 2.77). The most frequent reason for active HIV testing was “feeling sick” (42.4%, 255/601), and the main reason for never considering HIV testing was “never thinking of getting HIV” (74.0%, 542/732).ConclusionsLow‐risk perception and a lack of awareness of HIV‐related symptoms resulted in a high proportion of AHD in Guangdong Province, especially among the elderly, those diagnosed at medical facilities and those with low social support. Strengthening AIDS education and training programmes to scale up HIV testing through provider‐initiated testing and counselling in medical facilities and VCT could facilitate early HIV diagnosis.
Annexin A2 regulates unfolded protein response via IRE1–XBP1 axis in macrophages duringP. aeruginosainfection
Authors: Zhou Chuan-Min , Luo Li-Mei , Lin Ping , Pu Qinqin , Wang Biao , Qin Shugang , Wu Qun , Yu Xue-Jie , Wu Min 
Published: November 2020

AbstractPseudomonas aeruginosa is a severe Gram-negative opportunistic bacterium that causes a spectrum of organ system diseases, particularly in immunocompromised patients. This bacterium has been shown to induce unfolded protein response (UPR) during mammalian infection. Annexin A2 (AnxA2) is a multicompartmental protein relating to a number of cellular processes; however, it remains unknown whether AnxA2 coordinates a UPR pathway under bacterial infection conditions. Here, we report that the endoplasmic reticulum stress inositol-requiring enzyme 1 (IRE1)–X-box binding protein 1 (XBP1) pathway was up-regulated by AnxA2 through p38 MAPK signaling following P. aeruginosa infection in macrophages, whereas ATF4 and ATF6 not. In addition, XBP1 was found as a positive regulator of innate immunity to tame P. aeruginosa challenges by enhancing autophagy and bacterial clearance. XBP1 also facilitated NF-κB activation to elicit the release of proinflammatory cytokines predominantly in macrophages. Together, our findings identify AnxA2 as a regulator for XBP1-mediated UPR pathway.
SERSTEM: An app for the statistical analysis of correlative SERS and TEM imaging and evaluation of SERS tags performance
Authors: Lenzi Elisa , Litti Lucio , Jimenez de Aberasturi Dorleta , Henriksen‐Lacey Malou , Liz‐Marzán Luis M. 
Published: November 2020

AbstractRaman spectroscopy is becoming increasingly popular as an in vitro bioimaging technique, when coupled with plasmonic substrates such as gold nanoparticles (AuNPs). Plasmonic AuNPs not only display excellent biocompatibility but can also induce the surface‐enhanced Raman scattering (SERS) effect, which can be exploited for cell labeling, as an interesting alternative to fluorescence‐based techniques. SERS bioimaging requires the use of so‐called SERS tags or SERS‐encoded AuNPs. A remaining difficulty toward the general implementation of this method is the difficulty to correlate the SERS signal (spectral intensity) with the number of SERS tags. Therefore, a general correlation method, suitable for arbitrary AuNP morphologies and Raman‐active molecules (Raman reporters or RaRs), should largely improve the quantitative character of SERS as an imaging technique. We propose a protocol, with an associated app (SERSTEM), which enables the user to determine the average SERS intensity per nanoparticle from transmission electron microscopy (TEM) and SERS data. As a proof of concept, we demonstrated the method for Au nanostars and nanorods, carrying four different RaRs, and implemented the SERSTEM app, which is publicly available from an open‐source platform.
Step‐Economical Synthesis of Conjugated Polymer Materials Composed of Three Components: Donor, Acceptor, and π Units
Authors: Kuwabara Junpei , Kanbara Takaki 
Published: November 2020

AbstractConjugated polymers have immense potential for their use as semiconducting materials in organic optoelectronic devices. The improvement of synthetic methods for conjugated polymers is important for the practical application of conjugated polymers. For mass production, synthetic methods must be developed by considering the concerns regarding cost and environment. Reduction in the number of synthetic steps is an efficient approach to address these concerns. The utilization of direct CH functionalization is a reasonable strategy in monomer and polymer syntheses, because the prefunctionalization steps for CC bond formation can be eliminated. This review summarizes the recent developments in the efficient syntheses of conjugated polymers as well as their monomers via direct arylation (CH/CX coupling) and cross‐dehydrogenative coupling (CH/CH coupling) reactions.
Surface Modification of Parylene C Film via Buchwald–Hartwig Amination for Organic Solvent‐Compatible and Flexible Microfluidic Channel Bonding
Authors: Satheeshkumar Chinnadurai , Jung Bum‐Joon , Jang Hansol , Lee Wonhee , Seo Myungeun 
Published: November 2020

AbstractSurface modification offers an efficient and economical route to installing functional groups on a polymer surface. This work demonstrates that primary amine groups can be introduced onto a polymer surface via Buchwald–Hartwig amination, and the functionalized substrates can be chemically bonded to produce functional microfluidic devices. By activating the CCl bond in commercially used poly(chloro‐p‐xylylene) (parylene C) by Pd catalyst and substituting Cl with the amine source, the amine groups are successfully installed in a facile and recyclable manner. The substrates can be covalently bonded with each other via amine‐isocyanate chemistry, providing much higher bonding strength compared to previous methods based on noncovalent adhesive coatings. As a result, transparent and flexible microfluidic channels can be fabricated that are compatible with organic solvents and high pressure. Retention of amine group reactivity in the channel suggests the potential of this methodology for the surface immobilization of functional molecules for microfluidic reactors and biosensors.
Does the Country Make a Difference in Impulse Control Disorders? A Systematic Review
Authors: Parra‐Díaz Paloma , Chico‐García Juan Luis , Beltrán‐Corbellini Álvaro , Rodríguez‐Jorge Fernando , Fernández‐Escandón Clara Lastras , Alonso‐Cánovas Araceli , Martínez‐Castrillo Juan Carlos 
Published: November 2020

AbstractBackgroundImpulse control disorders (ICDs) have an increased frequency in patients with Parkinson's disease (PD), mainly because of treatment with dopamine agonists (DA). Factors related with the country of origin (culture, economy, healthcare politics) may impact phenomenology.ObjectivesTo explore phenomenology of ICDs depending on the country.MethodsA systematic review following PRISMA guidelines was performed using Pubmed database. Articles published up to 2018 in which the prevalence of ICDs was analyzed were selected.ResultsThirty‐two studies from 22 countries worldwide were included. The highest prevalence of ICDs in each continent was found in UK (59%), USA (39.1%) and India (31.6%). Frequency of ICDs was higher in those studies with lower mean age, higher proportion of males, whenever a screening instrument was used and whenever prescription of DAs was more common. Prevalence of ICDs was higher in Western countries compared to Asian countries (20.8% vs. 12.8%,P < 0.001) as it was the proportion of patients treated with DAs (66% vs. 48.2%,P < 0.001). Hypersexuality was the most common ICD overall (up to 23.8%). The highest frequencies of compulsive buying and eating were found in Western countries. Gambling was less commonly diagnosed, but prevalence was relevant Japan (14%).ConclusionWe observed a tendency towards a different ICD profile in different geographical areas, which may be attributable to socio‐economical, cultural or political influences in the phenomenology of these disorders. Acknowledging these differences could help their early detection, which is critical for prognosis.
A percentile‐based approach to rainfall scenario construction for surface‐water flood forecasts
Authors: Böing Steven J. , Birch Cathryn E. , Rabb Benjamin L. , Shelton Kay L. 
Published: November 2020

AbstractA novel technique to produce reasonable worst‐case rainfall scenarios from ensemble forecasts is presented. This type of scenario is relevant for predicting the risk of localized, intense rainfall events with a duration between 15 min and several hours. Such rainfall events can cause surface‐water (pluvial) flooding. Producing useful forecasts of these events at lead times of more than a few hours is challenging due to the precision and accuracy in rainfall intensity, duration and location that is required. The technique described here addresses these challenges by constructing appropriate scenarios using a neighbourhood technique in combination with ensemble forecasting. It is similar to the distance‐dependent depth–duration analysis described in earlier studies, but it introduces an additional post‐processing step based on probability distribution functions of rainfall accumulation near a location of interest. This additional step makes the reasonable worst‐case scenarios less dependent on grid‐scale behaviour, and helps to generate scenarios with a consistent interpretation. The method is used to compare forecasts with a lead time of 6–36 hr to radar data for several case studies that occurred in Yorkshire. These comparisons also introduce new techniques to present maps of the reasonable worst‐case rainfall accumulation at each location.
Clinicopathological and immunological characterization of RNA m6A methylation regulators in ovarian cancer
Authors: Wang Qingying , Zhang Qinyi , Li Qingxian , Zhang Jing , Zhang Jiawen 
Published: November 2020

AbstractBackgroundN6‐methyladenosine (m6A) modification is one of the critical gene regulatory mechanisms implicated in cancer biology. However, the roles of m6A regulators in ovarian cancer are still poorly understood.MethodsWe integrated multiple databases including Gene Expression Omnibus (GEO), ROC Plotter, Kaplan‐Meier Plotter, and Tumor Immune Estimation Resource (TIMER) to explore clinicopathological significance of m6A regulators in ovarian cancer.ResultsWe showed that alterations in the expression of m6A regulators were related to the malignancy and poor prognosis of ovarian cancer. We found decreased YTHDC1 and increased RBM15 expressions were associated with ovarian cancer cell metastases and HNRNPC was a predictor of paclitaxel resistance. Moreover, dysregulated m6A regulators were enriched in the activation of cancer‐related pathways. Our results further demonstrated that the level of immune cell infiltration and the expression of various immune gene markers were closely associated with the expressions of specific m6A regulators (RBM15B, ZC3H13, YTHDF1, and IGF2BP1).ConclusionsOur study establishes a new prognostic profile of ovarian cancer patients based on m6A regulators, and highlights the potential roles of m6A regulators in ovarian cancer development.
Oxytocin Administration Increases Proactive Control in Men with Overweight or Obesity: A Randomized, Double‐Blind, Placebo‐Controlled Crossover Study
Authors: Plessow Franziska , Marengi Dean A. , Perry Sylvia K. , Lawson Elizabeth A. 
Published: November 2020

ObjectivePreclinical and clinical evidence suggests that oxytocin administration decreases food intake and weight. The mechanisms underlying the anorexigenic effects of oxytocin in humans are unknown but critical to study to consider oxytocin as a neurohormonal weight loss treatment. Complementing ongoing research into metabolic and food motivation mechanisms of oxytocin, this study hypothesized that in humans, oxytocin improves cognitive control over behavior.MethodsIn a randomized, double‐blind, placebo‐controlled crossover study of 24‐IU single‐dose intranasal oxytocin, 10 men with overweight or obesity completed a stop‐signal task assessing ability and strategy to suppress behavioral impulses, in which they performed a choice‐reaction task (go task) but had to withhold their response when prompted (stop task). It was hypothesized that oxytocin would improve suppression of behavioral impulses.ResultsAfter receiving oxytocin, compared with placebo, participants showed increased reaction times in the go task (mean [M] = 936 milliseconds vs. 833 millseconds; P = 0.012; 95% CI: 29 to 178) and displayed fewer stop errors (M = 36.41% vs. 41.15%; P = 0.049; 95% CI: −9.43% to −0.03%).ConclusionsOxytocin triggers increased proactive control over behavior. Future studies need to further characterize the impact of oxytocin on cognitive control and investigate its potential role in the anorexigenic effects of oxytocin in human obesity.
Efficacy of FOLFOX in Patients with Aggressive Pancreatic Neuroendocrine Tumors After Prior Capecitabine/Temozolomide
Authors: Al-Toubah Taymeyah , Morse Brian , Pelle Eleonora , Strosberg Jonathan 
Published: November 2020

Abstract

Background
5-fluorouracil, leucovorin, and oxaliplatin (FOLFOX) has activity in pancreatic neuroendocrine tumors (pNETs), but its use is limited, partly because of toxicities. pNETs can often become aggressive over time. We evaluated the efficacy of FOLFOX in patients with aggressive pNETs who had progressed after capecitabine plus temozolomide (cap/tem) among other treatments.

Materials and Methods
This was a retrospective study of all patients with well-differentiated metastatic pNETs, treated at an academic cancer center between January 2008 and June 2019, who received FOLFOX and had received cap/tem in the past. The primary endpoint was objective response rate.

Results
Thirty-one patients met eligibility criteria. Twenty-five received FOLFOX, and six received FOLFOX with bevacizumab. Patients were heavily pretreated, having received a median of three prior lines of systemic therapy prior to FOLFOX (range, 1–8). Three (9.7%) patients had grade [G]1 tumors, 16 (51.6%) had G2, and 6 (19.4%) had G3, and grade was unspecified in 6 (19.4%) patients. Fourteen (45.2%) exhibited a best response of partial radiographic response per RECIST 1.1 criteria, 15 (48.4%) stable disease, and 2 (6.4%) progressive disease; overall response rate was 45.2% and disease control rate was 93.5%. Median progression-free survival was 6 months (95% confidence interval [CI], 5.0–7.0), and median overall survival was 16 months from onset of study treatment (95% CI, 11.3–20.7) and 67 months from date of diagnosis (95% CI, 49.8–84.2). Median duration of treatment was 3 months, and median duration of response was 2 months. Toxicity profile was consistent with known adverse events associated with this regimen.

Conclusion
FOLFOX is active in aggressive, heavily pretreated pNETs that have progressed on prior cap/tem chemotherapy; response durations are relatively short.

Implications for Practice
FOLFOX chemotherapy has robust activity in patients with rapidly progressive, heavily pretreated pancreatic neuroendocrine tumors (NETs), a setting in which few, if any, other options are likely to be effective. Durations of response, however, are relatively short, and new treatments are urgently needed for patients with aggressive transformation of pancreatic NETs.