bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Activated Murine Mast Cells, NFATc2 Is Critical for the Production of Autocrine IL-3, Thereby Promoting the Expression of IL-9
Authors: Sabbaghi Farhad , Ullner Lorenz , Bohn Toszka , Hahlbrock Jennifer , Bopp Tobias , Schmitt Edgar , Klein Matthias , Stassen Michael 
Published: December 2020

Abstract
IL-9 has lent its numerical designation to the Th9 subset of CD4+ Th cells, although it is also produced by additional cell types, including mast cells. It is a pleiotropic cytokine involved in allergic reactions, parasitic infections, autoimmune inflammation, and cancer immunity. In this article, we provide evidence that NFATc2 has contradictory functions in the expression of IL-9 in murine Th9 cells and bone marrow–derived mast cells (BMMC). The basis for this is our observation that the production of IL-9 in NFATc2-deficient Th9 cells is increased, whereas it is decreased in BMMC devoid of NFATc2. In addition, NFATc2 deficiency almost completely abrogates the expression of IL-3 in both cell types. However, selectively in BMMC, the production of IL-9 critically depends on autocrine IL-3 acting via the sustained activation of STAT5 on the expression of IL-9. Furthermore, we demonstrate that IL-3 acts independently and synergistically with IL-1β on the production of IL-9. Taken together, we highlight NFATc2-driven production of autocrine IL-3 as a critical and cell type–specific component for IL-9 expression in BMMC.
High-Sensitivity Assessment of Phagocytosis by Persistent Association-Based Normalization
Authors: de Neergaard Therese , Sundwall Martin , Wrighton Sebastian , Nordenfelt Pontus 
Published: December 2020

Abstract
Phagocytosis is measured as a functional outcome in many research fields, but accurate quantification can be challenging, with no robust method available for cross-laboratory reproducibility. In this study, we identified a simple, measurable parameter, persistent prey–phagocyte association, to use for normalization and dose-response analysis. We apply this in a straightforward analytical method, persistent association-based normalization, in which the multiplicity of prey (MOP) ratio needed to elicit half of the phagocytes to associate persistently (MOP50) is determined first. MOP50 is then applied to normalize for experimental factors, separately analyzing association and internalization. We use reference human phagocyte THP-1 cells with different prey and opsonization conditions to compare the persistent association-based normalization method to standard ways of assessing phagocytosis and find it to perform better, exhibiting increased robustness, sensitivity, and reproducibility. The approach is easily incorporated into most existing phagocytosis assays and allows for reproducible results with high sensitivity.
Expression of GM-CSF Is Regulated by Fli-1 Transcription Factor, a Potential Drug Target
Authors: Wang Xuan , Lennard Richard Mara , Li Pengfei , Henry Brittany , Schutt Steven , Yu Xue-Zhong , Fan Hongkuan , Zhang Weiru , Gilkeson Gary , Zhang Xian K 
Published: December 2020

Abstract
Friend leukemia virus integration 1 (Fli-1) is an ETS transcription factor and a critical regulator of inflammatory mediators, including MCP-1, CCL5, IL-6, G-CSF, CXCL2, and caspase-1. GM-CSF is a regulator of granulocyte and macrophage lineage differentiation and a key player in the pathogenesis of inflammatory/autoimmune diseases. In this study, we demonstrated that Fli-1 regulates the expression of GM-CSF in both T cells and endothelial cells. The expression of GM-CSF was significantly reduced in T cells and endothelial cells when Fli-1 was reduced. We found that Fli-1 binds directly to the GM-CSF promoter using chromatin immunoprecipitation assay. Transient transfection assays indicated that Fli-1 drives transcription from the GM-CSF promoter in a dose-dependent manner, and mutation of the Fli-1 DNA binding domain resulted in a significant loss of transcriptional activation. Mutation of a known phosphorylation site within the Fli-1 protein led to a significant increase in GM-CSF promoter activation. Thus, direct binding to the promoter and phosphorylation are two important mechanisms behind Fli-1–driven activation of the GM-CSF promoter. In addition, Fli-1 regulates GM-CSF expression in an additive manner with another transcription factor Sp1. Finally, we demonstrated that a low dose of a chemotherapeutic drug, camptothecin, inhibited expression of Fli-1 and reduced GM-CSF production in human T cells. These results demonstrate novel mechanisms for regulating the expression of GM-CSF and suggest that Fli-1 is a critical druggable regulator of inflammation and immunity.
KETIKA COVID -19 MENYERBU TAMAN DIDASKALOS
Authors: Muanley Yonas 
Published: December 2020

Abstract: This study aims to reveal the negative and positive sides of the spread of Covid-19 which does not rule out the possibility of entering the theological high school campus which in this article uses a theological technical term, namely taman didaskalos. Taman didaskalos is a technical term used to refer to the place where the activities of didaskalos (teachers / lecturers and prospective teachers) take place, namely the Theology College campus, especially the Santosa Asih IKSM Theological College. The method used is a qualitative research method using domain taxonomic data analysis. The theory used is the study of Covid-19 and didaskalos which is based on a number of theoretical-theological truths about the two concepts studied, namely Covid and Didaskalos. The findings were that the didaskalos, especially the didaskalos candidates, saw Covid Covid-19 as an enemy, while Covid-19 as a friend still needed deep thinking about Covid-19.
Keywords: Covid-19, Taman, Didaskalos

Abstrak: Penelitian ini bertujuan untuk mengungkapkan sisi negative dan positif dari penyebaran Covid-19 yang tidak menutup kemungkinan dapat memasuki kampus sekolah tinggi teologi yang di dalam artikel ini menggunakan istilah teknis teologis yakni taman didaskalos. Taman didaskalos adalah istilah teknis yang dipakai untuk merujuk pada tempat berlangsungnya kegiatan para didaskalos (guru/dosen dan calon guru) yaitu kampus Sekolah Tinggi Teologi khususnya Sekolah Tinggi Teologi IKSM Santosa Asih. Metode yang dipakai yakni metode penelitian kualitatif dengan menggunakan analisis data taksonomi domain. Teori yang digunakan yakni kajian tentang Covid-19 dan didaskalos yang didasarkan pada sejumlah kebenaran teoritik-teologis tentang dua konsep yang diteliti yaitu Covid dan didaskalos. Temuannya yakni para didaskalos khususnya calon didaskalos memandang Covid Covid-19 sebagai musuh, sementara Covid-19 sebagai kawan masih memerlukan pemikiran yang mendalam tentang Covid-19.
Kata Kunci: Covid-19, Taman, Didaskalos
PERANAN BAURAN PROMOSI TERHADAP PENINGKATAN VOLUME PEMBIAYAAN PADA PT BPRS RAHMANIA DANA SEJAHTERA BIREUEN DI KABUPATEN BIREUEN
Authors: Nurjannah Nurjannah 
Published: December 2020

Penelitian ini bertujuan untuk mengetahui pengaruh penerapan strategi pemasaran yang meliputi Periklanan (Advertising) Periklanan adalah semua bentuk penyajian nonpersonal, promosi ide-ide, promosi barang atau jasa yang dilakukan oleh sponsor yang dibayar, Promosi Penjualan (Sales Promotion) Promosi penjualan adalah variasi insentif jangka pendek untuk merangsang pembelian atau penjualan suatup roduk atau jasa, Hubungan Masyarakat dan Publisitas (Public Relation and Publicity), Hubungan Masyarakat adalah suatu usaha (variasi) dari rancangan program guna memperbaiki, mempertahankan, atau melindungi perusahaan atau citra produk, Penjualan Persoanal (Personal Selling), Penjualan pribadi atau tatap muka adalah penyajian lisan dalam suatu pembicaraan dengan satu atau beberapa pembeli potensial dengan tujuan untuk melakukan penjualan. Pemasaran Langsung (Direct Marketing) komunikasi secara langsung yang digunakan dari mail, telepon, fax, e-mail, atau internet untuk mendapatkan tanggapan langsung dari konsumen secara jelas.Sampel pada penelitian ini adalah data yang di peroleh pihak manajemen dan nasabah yang menggunakan produk pembiayaan di PT BPRS Rahmania Dana Sejahtera, dalam bentuk wawancara dan analisa data yang dimiliki oleh perusahaan.Kata kunci: Bauran Promosi, Peningkatan Volume Pembiayaan
Haematological stress parameters and behavioural characteristics of dairy type goat kids compared to indigenous breeds during an intensive fattening programme
Authors: Ergul Ekiz Elif , Yalcintan Hulya , Ekiz Bulent 
Published: December 2020

Abstract. In any production model, the extent to which the animals cope with the environment is important in terms of animal welfare and sustainability of production. The aim of the study was to investigate certain haematological parameters and behaviours of goat kids from dairy type Saanen and Maltese breeds via comparison with indigenous Hair and Gokceada breeds during the 10-week intensive fattening period. Eleven male goat kids each of Saanen, Maltese, Hair and Gokceada breeds were weaned at 3–3.5 months of age and then placed into four fattening pens prepared for each breed, separately. Cortisol, glucose and total protein levels were higher in Gokceada kids in the last period of the fattening compared to the kids of other breeds (P<0.05). In Gokceada kids, an evident decrease in the time spent hay feeding and on rumination and an increase in lying, standing and self-grooming behaviours were determined during the last 3 weeks of fattening. Moreover, there was a significant decrease regarding hay feeding in Hair goat kids in the last 2 weeks (P<0.05). Hair goat kids also exhibited less rumination behaviour compared to Saanen kids during the last 4 weeks of fattening (P<0.05). On the other hand, kids of dairy breeds did not express behavioural or biochemical stress responses during the fattening period. As a conclusion, when evaluated in terms of animal welfare, results of the current study may indicate that indigenous breeds, especially Gokceada kids, are not appropriate for intensive fattening in a pen.
Marco de referencia para la integración de la contabilidad social en la gestión estratégica de las empresas de Economía Social
Authors: Echanove Franco Alfonso 
Published: December 2020

La presión social e institucional al tejido empresarial por generar y evidenciar un valor más allá de sus resultados económicos se ha incrementado en los últimos años. A las demandas de información sobre los impactos positivos y negativos de la actividad empresarial se suma ahora la necesidad de plantear e implantar acciones de impacto social positivo. En este contexto las empresas de Economía Social se enfrentan a un reto importante. Por su naturaleza cabría atribuirles una mayor generación de valor social y, sin embargo, esta no puede evidenciarse exclusivamente a través de la formulación de sus aspiraciones sociales. Surge la necesidad de integrar de forma diferenciada la generación y distribución de valor social en su gestión estratégica. Lo anterior se sustenta en el presente artículo en un análisis comparativo entre empresas de Economía Social y empresas mercantiles sobre la gestión del valor social en dos hitos de sus procesos de gestión estratégica: la declaración de la intención estratégica y la medición de resultados. Del análisis se infiere la necesidad de contar con sistemas de información social robustos, que permitan la trazabilidad entre la definición del propósito de la organización y sus resultados y por ende, la comparativa entre organizaciones. La aplicación del modelo poliédrico de contabilidad social se ha considerado adecuada en estudios previos para la medición y comunicación del valor social generado por organizaciones de la Economía Social. Con el presente artículo se pretende incorporar un elemento novedoso al proponer su integración en los procesos de gestión estratégica.
Science and technology parks: measuring their contribution to society through social accounting
Authors: Blázquez Arenas Víctor , Aguado Ricardo , Retolaza José Luis 
Published: December 2020

Science and Technology Parks (STPs) have made a positive contribution to regional development in the last decades. In general, the assessment of their level of performance has been based on two major variables: number of new companies and number of new jobs created. In this paper we propose the use of social accounting to measure the social value (SV) generated by STPs as an additional tool to assess the level of contribution of STPs to social development. Social accounting could be of interest for policy makers and regional governments in order to evaluate regional STPs and their social performance. In addition, in this paper we present an innovative methodology for calculating SV. Instead of using primary data to calculate SV (through interviews or consultations with individual stakeholders), we propose the use of secondary data available in open databases to measure SV. In this way, the measurement of SV for a large number of individual organizations can be achieved using a limited amount of resources. In this line, and as a seminal implementation of this methodology, we calculate the SV generated by four STPs in two different regions and countries belonging to the European Union using secondary data. Thus, the main contribution of the paper is twofold: on one hand it proposes social accounting to assess the performance of STPs through the calculation of SV, and on the other hand it implements a new methodology to calculate SV which requires the use of a lesser number of resources.
Determining success factors in the implementation of social accounting
Authors: Lazkano de Anta Larraitz , Beraza Ana , San-José Leire 
Published: December 2020

The aim of this paper is to determine the success factors based on principal change driving forces when implementing social accounting into social companies, and by extension, gain an insight into the overall impact of social accounting, in other words the monetization of social value. It includes a quantitative analysis of the various effects involved in implementing social accounting in Social Economy companies: 1) improving relations with stakeholders; 2) the commitment of company employees; 3) an interest in comparing results with other companies that have also introduced social accounting; 4) networking with other organizations through social accounting. Twenty-seven percent of the social companies that apply social accounting participated in the study. It was applied at three points (prior to, immediately after and six months after applying social accounting), social accounting in order to compare expectations of perceptions regarding communication, strategy and results after implementing social accounting. The results of statistical analysis conducted by SPSS Statistics 26.0 suggest that the main reason that leads social companies to apply social accounting is to improve reputation. Likewise, they suggest that the more time passes, the greater the incidence of social accounting in communication and strategy. This study contributes to “Lewin’s change theory” whereby the change project is implemented by generating greater collaborator participation through empowerment in social economy entities, where employee participation is crucial.
POLITICA DE FORMAÇÃO DE ADOLESCENTES INFRATORES DA FUNASE/PE: PERSPECTIVA DOS PROFESSORES QUANTO A PROPOSTA EDUCACIONAL E A PRÁTICA PEDAGÓGICA
Authors: César Isaura De Albuquerque , Alencar Maria Fernanda Dos Santos 
Published: December 2020

Este artigo tem como objeto de estudo a formação de jovens e adolescentes responsáveis por atos infracionais acolhidos na Fundação de Atendimento Socioeducativo de Pernambuco (Funase-PE). Busca analisar a proposta educacional e a prática pedagógica da Funase-PE, na perspectiva dos docentes que atuam na referida instituição, tendo como parâmetro a formação cidadã dos socioeducandos, conforme preconiza a proposta pedagógica. De natureza qualitativa, a pesquisa dividiu-se em três tipos: bibliográfica, documental e de campo. Concluiu que a proposta educacional e a prática pedagógica da Funase-PE buscam, por meio do esforço docente, contribuir para a formação cidadã dos adolescentes e jovens infratores nela acolhidos. Palavras-chave: Educação. Formação de Jovens infratores. Política de formação.
O QUE É ISSO QUE SE MOSTRA: O FENÔMENO NA ANÁLISE TEXTUAL DISCURSIVA?
Authors: Galiazzi Maria do Carmo , Sousa Robson Simplicio de 
Published: December 2020

Apresentamos uma compreensão sobre o fenômeno na Análise Textual Discursiva (ATD), metodologia de análise difundida em pesquisas em Educação. A palavra aparece em todo o livro inaugural de ATD, no qual realizamos um exercício de análise com a própria metodologia. A interrogação perseguida foi O que é isso que se mostra: o fenômeno na ATD? Pretendemos ampliar a compreensão da palavra em um movimento descendente do conceito à palavra e também nos movimentarmos em um movimento ascendente da palavra ao conceito. Entendeu-se que não há um fenômeno que de início se mostre, mas a maior clareza dele se tem por meio da descrição fenomenológica em direção à interpretação hermenêutica. A imersão nos textos, sua desorganização em busca de insights que organizam novos sentidos levam à abstração e à comunicação dos horizontes compreensivos.
Effect of Biochar, FYM and Nitrogen on Uptake of Nutrients, Quality and Yield of Blackgram
Authors: Kadam Y. D. , Laharia G. S. , Age A. B. , Jadhao S. D. , Mali D. V. , Rakhonde O. S. , Sonune B. A. , Kankal D. S. , Dange N. R. 
Published: December 2020

The present investigation was conducted during Kharif season (2018-19) to study the interactive effect of biochar, FYM and nitrogen on uptake of nutrients, quality and yield of Blackgram at Research Farm, Department of Agronomy, Dr. PDKV, Akola. The experiment was laid out in Randomized Block Design with nine treatments and three replications by split plot design (RCBD). The highest yield and yield attributing characters of blackgram were observed with soil application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1 and nitrogen @ 20 kg ha-1. Nutrient content and uptake by blackgram was significantly highest in treatment where soil application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1 and nitrogen @ 20 kg ha-1 was applied and it was on par with Biochar 5 t ha-1 + FYM 5 t ha-1 + Nitrogen 20 kg ha-1 and FYM 10 t ha-1 + Nitrogen 20 kg ha-1. The test weight was significantly highest in treatment receiving soil application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1 and nitrogen @ 20 kg ha-1 and it was found to be on par with all other treatments expect treatment of control. Protein content of blackgram was increased (p<0.05) with the application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1 and nitrogen @ 20 kg ha-1.
TDP‐43 as structure‐based biomarker in amyotrophic lateral sclerosis
Authors: Beyer Léon , Günther René , Koch Jan Christoph , Klebe Stephan , Hagenacker Tim , Lingor Paul , Biesalski Anne‐Sophie , Hermann Andreas , Nabers Andreas , Gold Ralf , Tönges Lars , Gerwert Klaus 
Published: December 2020

AbstractPathologic alterations of Transactivation response DNA‐binding protein 43 kilo Dalton (TDP‐43) are a major hallmark of amyotrophic lateral sclerosis (ALS). In this pilot study, we analyzed the secondary structure distribution of TDP‐43 in cerebrospinal fluid of ALS patients (n = 36) compared to Parkinson´s disease patients (PD; n = 30) and further controls (Ctrl; n = 24) using the immuno‐infrared sensor technology. ALS patients could be discriminated from PD and Ctrl with a sensitivity/specificity of 89 %/77 % and 89 %/83 %, respectively. Our findings demonstrate that TDP‐43 misfolding measured by the immuno‐infrared sensor technology has the potential to serve as a biomarker candidate for ALS.
Multi‐Site Electrocatalysts Boost pH‐Universal Nitrogen Reduction by High‐Entropy Alloys
Authors: Zhang Dan , Zhao Huan , Wu Xueke , Deng Ying , Wang Zuochao , Han Yi , Li Hongdong , Shi Yue , Chen Xilei , Li Shaoxiang , Lai Jianping , Huang Bolong , Wang Lei 
Published: December 2020

AbstractElectrocatalytic nitrogen reduction reaction (NRR) has been an important area for many scientists. However, high voltage requirements, low NH3 yield, and poor stability remain the biggest challenges for NRR. Here, novel high‐entropy alloys RuFeCoNiCu nanoparticles with small size (≈16 nm) and uniformity, prepared in oil phase at atmospheric pressure and low temperature (≤250 °C) are reported for the first time and are applied to NRR. According to the experiments, there is a high NH3 yield at a low overpotential. It has a surprising NH3 yield of 57.1 µg h–1 (11.4 µg h–1 cm–2) at 0.05 V versus RHE in 0.1 m KOH, and the corresponding Faradaic efficiency reaches 38.5%, which is the electrocatalyst with the highest NH3 yield at the voltage of 0.05 V versus RHE reported so far. Similarly, the material also exhibits excellent electrochemical properties in other electrolytes such as 0.1 m Li2SO4, 0.1 m Na2SO4, and 0.1 m HCl electrolytes. Besides, after the 100 h test, only slightly diminished in activity. Theoretical calculation shows that Fe surrounded by alloy metals is the best site for N2 adsorption and activation. Co‐Cu and Ni‐Ru couples show an excellent capacity to surface hydrogenation at a low overpotential.
Graphene‐Based Sorbents for Multipollutants Removal in Water: A Review of Recent Progress
Authors: Yap Pei Lay , Nine Md Julker , Hassan Kamrul , Tung Tran Thanh , Tran Diana N. H. , Losic Dusan 
Published: December 2020

AbstractThe coexistence of multiple toxic water pollutants (heavy metals, organic dyes, oils, and organic solvents) limits the sustainable supply of clean water worldwide and urges the development of advanced water purification technology that can remove these contaminants simultaneously. Since its discovery, graphene‐based materials have gained substantial attention toward development of new‐generation sorbents for water purification. Despite several recently published reviews on water purification technology using graphene and its derivatives, there is still a gap in the review considering multiple water‐pollutant remediation using advanced graphene materials. In this review, in the first instance, a comparative structure–function–performance relationship between graphene‐based sorbents and the multipollutants in water is established. A fundamental correlation is made between the sorption performance for diverse pollutants in water with the more specific adsorption properties (surface area, pore size, type of functional groups, C/O, C/N, and C/S atomic ratio) of advanced graphene sorbents. Second, the underlying interaction mechanisms are uncovered between different classes of water pollutants using single graphene‐based sorbents. Third, the rational design of advanced multipollutant sorbents based on graphene is elaborated. The reality, challenges, and opportunities of advanced graphene materials as emerging sorbents for sustainable water purification technology are finally presented in the last section.