bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifikasi Bakteri dan Efektivitas Antibiotik dalam Pengencer untuk Mengontrol Pertumbuhan Bakteri pada Semen Sapi Friesian Holstein
Authors: Rabusin Muttaqinullah , Andriani Andriani , Arifiantini Raden Iis , Karja Ni Wayan Kurniani 
Published: January 2020

This study was designed to investigate the presence of bacterial species in Friesian Holstein (FH) bovine semen at the time of collection, processing and to assess the efficacy of two types of antibiotics combinations; penicillin and streptomycin (PS) and gentamycin, tylosin, lincomycin and spectinomycin (GTLS) in semen extender on bacterial control and quality of semen. For this purpose, three experiments were conducted. In experiment 1, identification of bacterial content in fresh semen which collected from 5 bovine ejaculates. In experiment 2, identification of bacterial content in skimmilk-eggyolk extender which were prepared in artificial insemination center, Lembang, Bandung. In experiment 3, identification of bacterial content in frozen thawed semen. In the result, some of bacterial species were isolated from the bovine semen. The GTLS combination of antibiotics may be incorporated into a freezing extender or protocol without compromising the post-thawed semen quality of FH bull spermatozoa. Three types of bacteria were found in fresh semen; Klebsiella sp., Micrococcus sp., and Pediococcus sp.. Three types of bacteria were found in semen extender; Enterobacter cloacae, Pseudomonas diminuta and Serratia plymutica. Two types of bacteria were found in frozen semen; Enterobacter cloacae and Serratia plymutica. In conclusion, antibiotics PS and GTLS were effective for controlling the growth of bacteria in frozen semen.
Respons Hematologi dan Kimia Darah Domba Lokal Indonesia Terhadap Stres Transportasi Selama 12 Jam (HAEMATOLOGICAL AND BLOOD BIOCHEMICAL RESPONSES TO 12 HOUR TRANSPORTATION STRESS IN IN LOCAL INDONESIAN SHEEP)
Authors: Sarmin Sarmin , Hana Amelia , Astuti Pudji , Febrianto Yuda Heru , Airin Claude Mona 
Published: January 2020

The objective of this study was to investigate the effect of transport 12 hours on haematological and some biochemical responses of local Indonesian sheeps. Ten adults (2.0-2.5 years) male local Indonesian sheeps BW adult 30-40 kg were transported by L-300 pickup truck starting at 3:00 pm to 3:00 am on the d 2 without stopping. Jugular blood were collecte-d at 7 am (-8 h pre- transportation as baseline), 15:00 immadiately after (0 h), 19:00 (4 h), 23:00 (8 h) , 03.00 (12 h ), and at 11.00 d 2 (+8 h) post- transportation as recovery period). Under the conditions of the present study, transport of local Indonesian sheeps significantly increased glucose during 4 h transport (P <0.05), increased eosinophil at 0 h (P <0.05) and no significant changed in erythrocytes, haemoglobin , monocytes, neutrophils, lymphocytes, leukocytes, packed cell volume (PCV), and total plasma protein (TPP) (P> 0.05). Our findings showed that transportation 12 h affected eosinophil during 0 h and glucose metabolism during 4 h of transportation, but did not any affect on some haematology and dehydration responses in local Indonesian sheeps.
Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide (FROZEN SPERM QUALITY OF BALI BULLS IN MODIFIED COCONUT WATER EXTENDER WITH DIFFERENT DIMETHYL SULFOXIDE CONCENTRATION)
Authors: Hine Thomas Mata , Uly Kirenius , Nalley Wilmientje Marlene , Armadianto Heri 
Published: January 2020

Dimethyl Sulfoxide (DMSO) is one type of cryoprotectant which has a low molecular weight so that it is easier to enter cells when cryopreservation. The purpose of this study was to explore the optimal concentration of DMSO in modified coconut water (mCW) extender that were able to maintain frozen sperm quality of bali bulls. Semen was collected from two four-year old bali bulls by artificial vagina. Good quality semen diluted with mCW (young coconut water + 20% egg yolk + 7.5 % moringa leaf extract) and supplemented by 3, 5, or 7% DMSO. Semen was filled into 0.25 ml ministraw, and was incubated in a refrigerator at 5°C for four hours, frozen on the surface of liquid nitrogen for 10 minutes and then dipped into liquid nitrogen. The quality of post thawing sperm was measured 24 hours later by placing the ministraw of frozen semen into water at 37oC for 30 seconds. Data were analyzed by analysis of variance and continued with Duncan test. Postthawing observations showed that bali bulls sperm cryopreserved at 3% DMSO yielded higher motility and viability (p<0.05) i.e. 36 and 44.15%, than DMSO 5% i.e. 18 and 23.65%, and DMSO 7% i.e. 7 and 12.62%. The recovery rate of sperm cryopreserved at 3% DMSO was also higher (p<0.05) than DMSO 5 and 7%, successively 45.65, 23.06, and 8.86%. The results of this study concluded that the optimal concentration of DMSO in mCW diluent to maintain frozen sperm quality of bali bulls was 3%.
Gambaran Histopatologi dan Populasi Bakteri Asam Laktat pada Duodenum Ayam Pedaging yang Diberi Sinbiotik dan Diinfeksi Escherichia coli (HISTOPATHOLOGY AND LACTIC ACID BACTERIA POPULATION IN DUODENUM WITH SINBIOTIC AND ESCHERICHIA COLI INFECTED BROILER
Authors: Daud Muhammad , Yaman Muhammad Aman , Zulfan Zulfan 
Published: January 2020

The research aims to study the use of synbiotics as feed additives in rations on describe lesions histopathology and population of lactic acid bacteria in duodenum infected with Escherichia coli. The study used 96 broiler Jumbo series A Cibadak strains which were maintained until the age of six weeks. The study was conducted by experimental method, using a completely randomized design (CRD) with eight treatments of rations and four replications. The broilers were devided into eight treatment diets: R1 (basal diet/control), R2 (basal diet + prebiotic 0.4%); R3 (basal diet + probiotic 108 CFU); R4 (basal diet + prebiotic 0.4% + probiotic 108 CFU); R5 (basal diet + infected with E. coli 104 CFU); R6 (basal diet + prebiotic 0.4% + infected with E. coli 104 CFU); R7 (basal diet + probiotic 108 CFU + infected with E. coli 104 CFU); and R8 (basal diet + prebiotic 0.4% + probiotic 108 CFU + infected with E. coli 104 CFU). The observed variables were histopathology based on lesio microscopic and lactic acid bacteria population in duodenum age 2, 4 and 6 weeks. Data was analyzed by using one-way analysis of variance then continued with Duncan test. The results showed that the use of probiotics, prebiotics and synbiotics significantly (P<0.05) decreased histopathological lesio score and increased the population of lactic acid bacteria in duodenum age 2, 4 and 6 weeks. In conclusion the use of synbiotics as feed additives in the ration a positive on describe lesions histopathological and increase in the population of lactic acid bacteria in duodenum age 2, 4 and 6 weeks infected and not infected with E. coli.
Uji pigmen dan deteksi kapsul polisakarida pada Staphylococcus aureus isolat asal broiler PIGMENT TEST AND DETECTION OF POLISAKARIDA CAPSULES ON Staphylococcus aureus ISOLAT BROILER
Authors: Khusnan Khusnan , Kusmanto Dwi 
Published: January 2020

Staphylococcus aureus is a pathogenic bacterium causing disease in humans and animals. In broilers it cause septicemia, tendosinovitis, dermatitis, endocarditis, wound infections and arthritis and bumblefoot. The ability of Staphylococcus aureus to cause disease depends on the virulence factors they bear. The purpose of this research is to investigate the distribution of pigment production type and the existence of genes of polysaccharide capsule phenotype and genotype as determinant factor of virulence of bacteria on 15 isolates of Staphylococcus aureus from broiler. Pigment production test showed that 86.7% of isolates producing yellow pigment and 13.3% isolates produce orange pigment. The detection of polysaccharide capsules was phenotypically performed with hydrophobicity test with serum soft agar medium (SSA) showed 53.3% isolate grow compact and 46,7% isolate grown difuse and hydrophobicity test by salt aggregation test method (SAT) showed 66,7% hydrophil and 33.3% are hydrophobic. Genotype detection of polysaccharide capsule genes by polymerase chain reaction (PCR) showed 66.7% detected cap5 (amplicon 361bp) and 33.3% detected cap8 (173bp ampliole). The type of pigment production and the presence of polysaccharide capsules are some of the virulent factors in Staphylococcus aureus.
Efek Regional dan Cardiopulmonary Penggunaan Lidocaine dan Lidocaine-Xylazine pada Blokade Nervus Ischiadicus Domba (REGIONAL AND CARDIOPULMONARY EFFECTS OF LIDOCAINE AND LIDOCAINEXYLAZINE IN SHEEP SCIATIC NERVE BLOCK)
Authors: Napitu Herawati , Noviana Deni , Soehartono Raden Harry , Nurhidayat Nurhidayat 
Published: January 2020

General anaesthesia has a high risk during surgery, regional anaesthesia with nerve block is expected to minimize the use of general anaesthesia while increase the analgesic effect and muscle relaxation effect. This study aimed to compare the analgesic effects of sciatic nerve block on the hind limb and the effect of lidocaine 2%, and a combination of 2% lidocaine with 2% xylazine on sheep cardiopulmonary function. Sciatic nerve block was guided with ultrasonography and nerve stimulator. In this research, sheep were sedated with diazepam (0.5 mg / kg). Sheep was divided into three groups; first injected with saline solution (SS), second with lidocaine 2% 5 mg / kg (Lid) and third with lidocaine 2% 5 mg / kg and xylazine 2% 0.1 mg / kg (LidXyl). The results of this research showed that the LidXyl group had the highest analgesic effect and broader blockade area, namely femoris, cruris, and metatarsus-digit. The LidXyl blockade value was highest in the digitmetatarsus region, while the Lid group shows the highest blockade value in the cruris region. The LidXyl group with the addition of xylazine results in a decrease in heart rate and respiration rate. Xylazine increased and extended analgesic effect compared to lidocaine but resulted in a decrease in heart rate and respiration rate in the early minutes of anaesthesia, it is however safe to be used in sheep.
Impact of Dietary Fatty Acids on Macrophage Lipid Metabolism, Signaling and Function
Authors: Davanso Mariana Rodrigues , Crisma Amanda Rabello , Murata Gilson , Newsholme Philip , Curi Rui 
Published: January 2020

Abstract
Leukocytes are potent regulators of adipose tissue biology and whole-body metabolic homeostasis. In lean, non-obese conditions (insulin-sensitive), adipose tissue has innate and adaptive immune cells, including eosinophils, regulatory T cells, invariant NK cells, and M2 macrophages. A vast expansion in adipose tissue occurs in obesity, and this is associated with a marked alteration in the tissue leukocyte profile. There is a marked increase in B cells, CD8+ T cells, NK cells, neutrophils, and M1 macrophages. This condition induces a state of low-grade, chronic inflammation in the adipose tissue, which disrupts whole-body metabolism. Macrophages were the first leukocyte to be discovered in adipose tissue. Due to their proximity to nearby adipocytes, the macrophages are exposed to high levels of fatty acids and other lipids reported in obesity. Lipid uptake by tissue-resident macrophages is essential for their biological actions. Specifically, lipid uptake and metabolism, particularly of long-chain saturated fatty acids, activate inflammatory signaling pathways, potentiating adipose tissue inflammation, and metabolic dysfunction. Obesity exhibits increased fatty acid levels within the adipose tissue microenvironment. The increased lipid accumulation in the resident macrophages reflects the fatty acid composition of the adipocytes. The dietary fatty acid determines the fatty acid composition of the adipose tissue. Macrophages then accumulate fatty acids indirectly provided by the diet. The composition varies with the acyl chain length, e.g., short-, medium-, or long-chain, and saturated fatty acids. These fatty acids have wide-ranging effects on macrophages. We described herein in detail the impact of the different dietary fatty acids on macrophage functions. Shortly, long-chain saturated fatty acids are pro-inflammatory, whereas medium-chain fatty acids are relatively benign. Long-chain unsaturated fatty acids often antagonize the pro-inflammatory effects of long-chain saturated fatty acids.
Penyuluhan Kesehatan Masyarakat untuk Meningkatkan Kesehatan Keluarga di Desa Krajan Sukoharjo
Authors: Rukayah Rukayah , Poerwanti Jenny Indrastoeti Siti , Istiyati Siti , Sularmi Sularmi , Marmoah Sri 
Published: January 2020

Pengabdian ini ditujukan untuk anggota PKK Desa Krajan Sukoharjo dalam rangka meningkatkan kesehatan keluarga. Tujuan pengabdian ini adalah agar para anggota PKK dapat mengetahui manfaat dalam menjaga kebersihan lingkungan masyarakatdan mampu meningkatkandan menjaga kebersihan lingkungan masyarakat terutama keluarga. Pengabdian ini dilaksanakan melalui metode ceramah dan diskusi. Hasil pengabdian sampai awal September 2019 ini adalah 1) terlaksananya pemaparan tentang kesehatan kepada ibu-ibu PKK di DesaKrajan Kecamatan Gatak Kabupaten Sukoharjo untuk meningkatkan kesehatan keluarga; 2) tanya jawab dan diskusi tentang kesehatan keluarga; 3) simulasi membersihkan ruangan tempat pertemuan dan dilanjutkan ke halamannya, kemudian dipisah-pisah antara sampah organik dan non organik.
Upaya Meningkatkan Pembelajaran Berorientasi HOTS Bagi Guru Matematika SMP Kota Surakarta dengan Pemanfaatan Hasil Program AKSI for School
Authors: Sujadi Imam , Kurniawati Ira , Wulandari Arum Nur 
Published: January 2020

Era revolusi industri 4.0 adalah suatu era dimana teknologi informasi telah menjadi basis dalam kehidupan manusia, karena dipengaruhi oleh perkembangan internet dan teknologi digital. Tantangan yang harus dihadapi dalam perubahan hidup di era disrupsi ini adalah peningkatan kualitas sumber daya manusia yang mampu bersaing di era tersebut dengan bekal ketrampilan berpikir tingkat tinggi. Penelitian ini dilakukan diawali dengan mensosialisasikan kebijakan pemerintah tentang Program AKSI for school kepada Guru agar mereka mampu memanfaatkan program AKSI untuk melakukan pembelajaran yang berorientasi Pada HOTS. Kegiatan terdiri dari empat tahap, yaitu: (1) pembekalan tentang kebijakan pendidikan, perencanaan dan penilaian pembelajaran matematika berorientasi HOTS, (2) pelatihan penggunaan program AKSI for School, (3) pelatihan identifikasi kesulitan siswa dalam menyelesaikan soal HOTS pada program AKSI for School, dan (4) pelatihan pengembangan pembelajaran untuk mengatasi kesulitan siswa dalam menyelesaikan soal HOTS. Hasil kegiatan ini menunjukkan bahwa guru guru MGMP matematika mampu menggunakan program AKSI for school dengan baik, mampu meningkatan pemahaman Guru matematika tentang karakteristik level soal level Knowing, Applying dan Reasoning, serta meningkatan kemampuan Guru dalam merancang pembelajaran matematika di SMP yang berorientasi pada HOTS.<p> </p>
Penerapan Teknologi Image Processing untuk Optimalisasi Petik Merah pada Kebun Kopi Rakyat
Authors: Budianto Aris , Budiyanto Cucuk , Fauzan Qois Amin , Widiastuti Indah , Maryono Dwi 
Published: January 2020

<p class="HeaderAbs"><strong>Abstract</strong></p><p class="Abstract">Coffee has been cultivated as the secondary produce for decades in Girimarto, Wonogiri, however, the selective picking practice remain alient for local farmers. Selective picking is considered impractical due to time consumption and laborous work that farmers should carry out. The community service project designs and implements image recognition technology to help acquire coffee-cheery ripeness condition. Adopting a geolocation, the appropriate routing strategies, would enable farmers to selectively pick the red cherries in a systematic sequence. The image processing technology was applied by adopting Raspberry Pi microcomputer, <em>Raspberry Pi Camera Board, </em>version <em>2</em>, and OpenCV programming language. The transition to selective picking and the subsequent post-harvest technology would likely produce high-quality green bean coffee. It is expected that the income of smallholder coffee farmers will gradually be increasing.</p><br /><p class="HeaderAbs"><strong>ABSTRAK</strong></p><p class="Abstract">Praktek petik buah merah dalam budidaya kopi belum menjadi prosedur baku pada perkebunan kopi rakyat di wilayah Girimarto, Wonogiri. Petani menganggap praktek petik merah pada panen buah kopi merepotkan dan memakan waktu karena dalam satu kunjungan ke kebun mereka hanya memetik buah kopi yang benar-benar matang. Pengabdian ini mendesain dan menerapkan perangkat pemantau kematangan buah kopi untuk membantu petani memperoleh informasi lokasi buah matang dan estimasi jumlahnya sehingga petani bisa merencanakan jalur pemetikan kopi berdasarkan lokasi batang pohon kopi tingkat kematangannya. Teknologi <em>image processing</em> diterapkan dengan mengadopsi penggunaan komputer mikro <em>Raspberry Pi</em>, modul kamera <em>Raspberry Pi Camera Board, </em>versi <em>2</em>, dan bahasa pemrograman <em>OpenCV. </em>Perubahan pola panen buah kopi dari petik sembarang (petik racutan_ menjadi panen petik merah diikuti dengan perbaikan proses fermentasi buah kopi diharapkan menghasilkan kualitas green bean menjadi lebih baik dan harga jual yang lebih tinggi.</p>
EVALUASI KURIKULUM PELATIHAN DALAM MENINGKATKAN KOMPETENSI PEDAGOGIK GURU PAI DI BALAI DIKLAT KEAGAMAAN JAKARTA
Authors: Bahrissalim Bahrissalim , Fauzan Fauzan 
Published: January 2020

<p><strong> </strong>This study aims to analyze the curriculum of PAI SD teacher training at Jakarta Religious Training Center, to identify the qualifications of the teaching staff and the Training participants at the Jakarta Religious Training Center, to review the implementation process of the curriculum at the Jakarta Religious Training Center, and to analyze the results of the improvement of pedagogic competence after the participants attended the training. The method used in this research is survey method using evaluative research type with CIPP model (context, Input, Process, Product). Data collection techniques used were observation, interview, questionnaire, document analysis and test. This research found: 1) Design of curriculum Training of Islamic Primary School Education teachers need to be improved formulation of training objectives and competence of Training participants, 2) Teachers Training of Islamic Primary School Teachers Education teachers meet the qualifications determined by R &amp; D Institute of Ministry of Religious Affairs. 3) The qualification of the participants of the Teacher Training for Islamic Primary School Education is not in accordance with the stipulations set out by the IAARD and the Ministry of Religious Affairs, 4) The evaluation of post-training process and evaluation needs to be improved and supplemented with monitoring and evaluation, and mentoring program. 5) The process of education and training at Jakarta Religious Training Center can improve the competence of teachers of Islamic Religious Education of Elementary School in DKI Jakarta.<strong></strong></p>
Severe Wound Complication due to Prineo Surgical Dressing in Shoulder Hemiarthroplasty
Authors: Pate Ryan C. , Neumeister Michael W. 
Published: January 2020

Case:
A 61-year-old woman with left shoulder osteoarthritis underwent a left shoulder hemiarthroplasty. The wound was closed using the Dermabond Prineo system. The patient had a severe and progressive reaction during the first 2 weeks that necessitated debridement and skin grafting. Three months after surgery, the patient was doing well.

Conclusions:
Severe reactions to 2-octyl cyanoacrylate are uncommon. The severity of the presented reaction is rare. However, surgeons using this closure method should be aware of potential reactions that may initially mimic infection. It is important to initiate appropriate treatment including removing the dressing, topical steroids, and/or oral antihistamines, antibiotics, and possible surgical wound debridement.
Bilateral Acetabular Fractures Associated with Seizures
Authors: Yngstrom Korey , Stapleton Erik , Aberman Zachary , Galos David 
Published: January 2020

Cases:
Two elderly women with significant medical comorbidities presented to a community hospital setting after new-onset generalized seizure activity while in bed without a history of trauma. Both were diagnosed by computed tomography scan with bilateral acetabular fractures. Owing to altered mental status from a postictal period, both were unable to be reliably assessed for pain, which resulted in a diagnostic delay.

Conclusions:
These cases highlight the importance of a proper secondary survey on mentally altered patients even without a history of significant trauma, a high index of suspicion for associated injuries in seizure patients, and the use of appropriate advanced imaging.
Reamed Intramedullary Nailing of a Femur Fracture in a Polytraumatized Patient on Extracorporeal Membrane Oxygenation
Authors: Bosch Liam , Ruoss Stephen , Bishop Julius 
Published: January 2020

Case:
We present the case of a young adult with blunt lung trauma and acute respiratory distress syndrome requiring extracorporeal membrane oxygenation (ECMO) after a motor vehicle crash with a concomitant diaphyseal femur fracture who underwent reamed intramedullary nailing (IMN) while on ECMO support.

Conclusion:
This case is important because it demonstrates that reamed IMN of the femur has been performed safely in a critically ill patient on ECMO.
Femoropatellar Osteoarthritis and Trochlear Femoral Bone Defect due to Giant Cell Tumor of the Knee: A Selected Patellofemoral Joint Arthroplasty and Reconstructive Technique
Authors: Conti Andrea , Boffano Michele , Pellegrino Pietro , Ratto Nicola , Sabatini Luigi , Piana Raimondo 
Published: January 2020

Case:A 35-year-old man with a giant cell tumor involving the lateral condyle and trochlea of the right distal femur underwent curettage of the lesion and cement grafting, which resulted in symptomatic patellofemoral osteoarthritis after more than 4 years. A standard follow-up excluded recurrence, whereas infection was ruled out while investigating the symptoms. Finally, a patellofemoral resurfacing prosthesis was implanted while filling the bone defects with tantalum cones.Conclusion:A “tailored” surgical technique with a selected patellofemoral joint arthroplasty could be used in oncologic setting to save further bone stock for possible revisions while permitting full and quick clinical recovery.
Team Approach: Evaluation and Management of Low-Grade Cartilaginous Lesions
Authors: Sullivan Connor W. , Kazley Jillian M. , Murtaza Hamza , Cooley Michael , Jones David , DiCaprio Matthew R. 
Published: January 2020

» Assessment of chondral lesions begins with a clinical evaluation and radiographs.» Longitudinal follow-up with serial radiographs is appropriate in cases without evidence of aggressive radiographic features.» Concerning radiographic features include periosteal reaction, soft-tissue extension, cortical destruction, endosteal scalloping of greater than two-thirds of the native cortex, larger lesion size (≥5 cm), and location in the axial skeleton.» Biomarkers such as IMP3, SOX4, microRNA, and periostin may be used as an adjunct in histologic assessment to help differentiate benign enchondroma from a low-grade chondrosarcoma.» Advanced-imaging studies, such as computed tomography (CT), bone scans, magnetic resonance imaging (MRI), dynamic contrast-enhanced MRI, and fluorodeoxyglucose positron emission tomography (FDG-PET), may be considered for borderline cases.» Aggressive or concerning radiographic features should prompt evaluation with advanced imaging or referral to an orthopaedic oncologist.