bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neural signal analysis with memristor arrays towards high-efficiency brain–machine interfaces
Authors: Liu Zhengwu , Tang Jianshi , Gao Bin , Yao Peng , Li Xinyi , Liu Dingkun , Zhou Ying , Qian He , Hong Bo , Wu Huaqiang 
Published: August 2020

AbstractBrain-machine interfaces are promising tools to restore lost motor functions and probe brain functional mechanisms. As the number of recording electrodes has been exponentially rising, the signal processing capability of brain–machine interfaces is falling behind. One of the key bottlenecks is that they adopt conventional von Neumann architecture with digital computation that is fundamentally different from the working principle of human brain. In this work, we present a memristor-based neural signal analysis system, where the bio-plausible characteristics of memristors are utilized to analyze signals in the analog domain with high efficiency. As a proof-of-concept demonstration, memristor arrays are used to implement the filtering and identification of epilepsy-related neural signals, achieving a high accuracy of 93.46%. Remarkably, our memristor-based system shows nearly 400× improvements in the power efficiency compared to state-of-the-art complementary metal-oxide-semiconductor systems. This work demonstrates the feasibility of using memristors for high-performance neural signal analysis in next-generation brain–machine interfaces.
A fine resolution dataset of accessibility under different traffic conditions in European cities
Authors: Christodoulou Aris , Dijkstra Lewis , Christidis Panayotis , Bolsi Paolo , Poelman Hugo 
Published: August 2020

AbstractUrban accessibility and congestion indicators allow us to benchmark cities. If these indicators are also available at a fine resolution, we can compare different neighbourhoods within a city. We present a dataset of different accessibility indicators for all urban areas with more than 250 thousand people in the EU27, the UK, Switzerland and Norway. Each city is analysed by means of a population grid of 500 m by 500 m and represented by a wider area covering both the densely populated urban centre and the commuting zone. To capture congestion, we measure accessibility for each grid cell at different times of the day that correspond to different traffic conditions using the detailed network and congestion information provided by TomTom.
Experimental investigation of the dynamic cavitation behavior and wall static pressure characteristics through convergence-divergence venturis with various divergence angles
Authors: Liu Yinan , Fan Hua , Wu Di , Chen Haijun , Feng Kuolei , Zhao Chaocheng , Wu Dongyin 
Published: August 2020

AbstractAs a highly efficient and energy-saving cavitation method, Venturi cavitation is widely used in many industrial fields. This study synchronously investigated the cavity behavior and its corresponding wall static pressure characteristics in Venturi channels with various divergence angles to research the role of the divergence angle in cavity shape and the wall static pressure oscillation. Five rectangular Venturi channels with different divergence angles (4°, 6°, 8°, 10°, and 12°) were tested at the cavitation number (0.3–1.0). Based on the dynamic behaviour of gas–liquid interface, three cavity shedding types were identified: front shedding (I), central shedding (II) and tail shedding (III). A modified correlation for predicting average cavity length was proposed with the consideration of the effect of the divergence angle. Combined with the wall static pressure characteristics, as the divergence angle increased, the wall static pressure fluctuation in the Venturi became more intense. According to the wall static pressure oscillation characteristics, for the larger divergence angles (θ = 6°, 8°, 10° and 12°), the wall static pressure oscillation frequency was the same as the cavity shedding frequency and increased with the increase of the divergence angle. For smaller divergence angle (θ = 4°), no definite periodicity in pressure oscillation frequency could be observed.
New water-soluble colorimetric pH and metal ione sensor based on graphene quantum dot modified with alizarine red S
Authors: Ahmadi Hassan , Keshipour Sajjad , Ahour Fatemeh 
Published: August 2020

AbstractA new colorimetric sensor was designed for the screening pH changes in solutions, as well as, detection of some cations. The sensor preparation includes the chemical binding of alizarine red S (ARS) as a sensor of pH and cation to graphene quantum dots (GQD). Loading ARS on GQD led to the formation of water soluble sensor which finally responded to the colorimetric detection of some cations in water. Solubility and stability of the sensor in water indicate that the sensor is an ideal system for the biological and environmental applications. To demonstrate the applicability of the new sensor, the colorimetric responds of sensor were examined for some cations including Fe3+, Co2+, Ca2+, As3+, Cd2+, Hg2+, Pb2+, Sn2+, Al3+, and Cr3+. The colorimetric detections of all the ions were performable individually in a solution. In addition, GQD-ARS as a colorimetric sensor detected Co2+ at pH < 0.6 with limit of quantification 0.08 mM and Fe3+ at 0.6 < pH < 4.0 with limit of quantification 0.03 mM in the mixture of cations.
TRPV4 activates the Cdc42/N-wasp pathway to promote glioblastoma invasion by altering cellular protrusions
Authors: Yang Wei , Wu Peng-fei , Ma Jian-xing , Liao Mao-jun , Xu Lun-shan , Yi Liang 
Published: August 2020

AbstractThe invasion ability of glioblastoma (GBM) causes tumor cells to infiltrate the surrounding brain parenchyma and leads to poor outcomes. Transient receptor potential vanilloid 4 (TRPV4) exhibits a remarkable role in cancer cell motility, but the contribution of TRPV4 to glioblastoma metastasis is not fully understood. Here, we reported that TRPV4 expression was significantly elevated in malignant glioma compared to normal brain and low-grade glioma, and TRPV4 expression was negatively correlated with the prognosis of glioma patients. Functionally, stimulation of TRPV4 promoted glioblastoma cell migration and invasion, and repression of TRPV4 hindered the migration and invasion of glioblastoma cells in vitro. Molecularly, TRPV4 strongly colocalized and interacted with skeletal protein-F-actin at cellular protrusions, and TRPV4 regulated the formation of invadopodia and filopodia in glioblastoma cells. Furthermore, the Cdc42/N-wasp axis mediated the effect of TRPV4-regulated cellular protrusions and invasion. Foremost, TRPV4 inhibitor treatment or downregulation of TRPV4 significantly reduced the invasion-growth of subcutaneously and intracranially transplanted glioblastoma in mice. In conclusion, the TRPV4/Cdc42/wasp signaling axis regulates cellular protrusion formation in glioblastoma cells and influences the invasion-growth phenotype of glioblastoma in vivo. TRPV4 may serve as a prognostic factor and specific therapeutic target for GBM patients.
MRI-based radiomics in breast cancer: feature robustness with respect to inter-observer segmentation variability
Authors: Granzier R. W. Y. , Verbakel N. M. H. , Ibrahim A. , van Timmeren J. E. , van Nijnatten T. J. A. , Leijenaar R. T. H. , Lobbes M. B. I. , Smidt M. L. , Woodruff H. C. 
Published: August 2020

AbstractRadiomics is an emerging field using the extraction of quantitative features from medical images for tissue characterization. While MRI-based radiomics is still at an early stage, it showed some promising results in studies focusing on breast cancer patients in improving diagnoses and therapy response assessment. Nevertheless, the use of radiomics raises a number of issues regarding feature quantification and robustness. Therefore, our study aim was to determine the robustness of radiomics features extracted by two commonly used radiomics software with respect to variability in manual breast tumor segmentation on MRI. A total of 129 histologically confirmed breast tumors were segmented manually in three dimensions on the first post-contrast T1-weighted MR exam by four observers: a dedicated breast radiologist, a resident, a Ph.D. candidate, and a medical student. Robust features were assessed using the intraclass correlation coefficient (ICC > 0.9). The inter-observer variability was evaluated by the volumetric Dice Similarity Coefficient (DSC). The mean DSC for all tumors was 0.81 (range 0.19–0.96), indicating a good spatial overlap of the segmentations based on observers of varying expertise. In total, 41.6% (552/1328) and 32.8% (273/833) of all RadiomiX and Pyradiomics features, respectively, were identified as robust and were independent of inter-observer manual segmentation variability.
The effects of Piper sarmentosum aqueous extracts on zebrafish (Danio rerio) embryos and caudal fin tissue regeneration
Authors: Zainol Abidin Intan Zarina , Fazry Shazrul , Jamar Nur Hidayah , Ediwar Dyari Herryawan Ryadi , Zainal Ariffin Zaidah , Johari Anis Nabilah , Ashaari Nur Suhanawati , Johari Nor Azfa , Megat Abdul Wahab Rohaya , Zainal Ariffin Shahrul Hisham 
Published: August 2020

AbstractIn Malaysia,Piper sarmentosumor ‘kaduk’ is commonly used in traditional medicines. However, its biological effects including in vivo embryonic toxicity and tissue regenerative properties are relatively unknown. The purpose of this study was to determine zebrafish (Danio rerio) embryo toxicities and caudal fin tissue regeneration in the presence ofP. sarmentosumaqueous extracts. The phytochemical components and antioxidant activity of the extract were studied using GC–MS analysis and DPPH assay, respectively. Embryo toxicity tests involving survival, heartbeat, and morphological analyses were conducted to determineP. sarmentosumextract toxicity (0–60 µg/mL); concentrations of 0–400 µg/mL of the extract were used to study tissue regeneration in the zebrafish caudal fin. The extract contained several phytochemicals with antioxidant activity and exhibited DPPH scavenging activity (IC50 = 50.56 mg/mL). Embryo toxicity assays showed that a concentration of 60 μg/mL showed the highest rates of lethality regardless of exposure time. Slower embryogenesis was observed at 40 µg/mL, with non-viable embryos first detected at 50 µg/mL. Extracts showed significant differences (p < 0.01) for tissue regeneration at all concentrations when compared to non-treated samples. In conclusion,Piper sarmentosumextracts accelerated tissue regeneration, and extract concentrations at 60 µg/mL showed the highest toxicity levels for embryo viability.
The proanthocyanin-related transcription factors MYBC1 and WRKY44 regulate branch points in the kiwifruit anthocyanin pathway
Authors: Peng Yongyan , Thrimawithana Amali H. , Cooney Janine M. , Jensen Dwayne J. , Espley Richard V. , Allan Andrew C. 
Published: August 2020

AbstractThe groups of plant flavonoid metabolites termed anthocyanins and proanthocyanins (PA) are responsible for pigmentation in seeds, flowers and fruits. Anthocyanins and PAs are produced by a pathway of enzymes which are transcriptionally regulated by transcription factors (TFs) that form the MYB-bHLH-WD40 (MBW) complex. In this study, transcriptomic analysis of purple-pigmented kiwifruit skin and flesh tissues identified MYBC1, from subgroup 5 of the R2R3 MYB family, and WRKY44 (highly similar to Arabidopsis TTG2) as candidate activators of the anthocyanin pathway. Transient over-expression of MYBC1 and WRKY44 induced anthocyanin accumulation in tobacco leaves. Dual luciferase promoter activation assays revealed that both MYBC1 and WRKY44 were able to strongly activate the promoters of the kiwifruit F3′H and F3′5′H genes. These enzymes are branch points of the pathway which specifies the type of anthocyanin accumulated. Stable over-expression of MYBC1 and WRKY44 in kiwifruit calli activated the expression of F3′5′H and PA-related biosynthetic genes as well as increasing levels of PAs. These results suggest that while previously characterised anthocyanin activator MYBs regulate the overall anthocyanin biosynthesis pathway, the PA-related TFs, MYBC1 and WRKY44, more specifically regulate key branch points. This adds a layer of regulatory control that potentially balances anthocyanin and PA levels.
Electrowetting-on-dielectric characteristics of ZnO nanorods
Authors: Kim Jae-Hun , Lee Jae-Hyoung , Mirzaei Ali , Kim Hyoun Woo , Tan Boon Teoh , Wu Ping , Kim Sang Sub 
Published: August 2020

AbstractHerein, we report the electrowetting-on-dielectric (EWOD) characteristics of ZnO nanorods (NRs) prepared via the hydrothermal method with different initial Zn2+ concentrations (0.03, 0.07, and 0.1 M). Diameter of the resultant ZnO NRs were 50, 70 and 85 nm, respectively. Contact angle (CA) measurements showed that the Teflon-coated ZnO NRs with diameters of 85 nm prepared from the 0.1 M solution had the highest CA (137°). During the EWOD studies, on the application of a voltage of 250 V, the water CA decreased to 78°, which demonstrates the potential application of this material in EWOD electronics. Furthermore, we explained the relationship between the applied voltage and CA based on the substrate nanostructures and our newly developed NR-on-film wetting model. In addition, we further validated our model by introducing the homo-composite dielectric structure, which is a composite of thin layered ZnO/Teflon and nano-roded ZnO/Teflon.
The α2 Na+/K+-ATPase isoform mediates LPS-induced neuroinflammation
Authors: Leite J. A. , Isaksen T. J. , Heuck A. , Scavone C. , Lykke-Hartmann K. 
Published: August 2020

AbstractNa+/K+-ATPase is a transmembrane ion pump that is essential for the maintenance of ion gradients and regulation of multiple cellular functions. Na+/K+-ATPase has been associated with nuclear factor kappa B (NFκB) signalling, a signal associated with lipopolysaccharides (LPSs)-induced immune response in connection with activated Toll-like receptor 4 (TLR4) signalling. However, the contribution of Na+/K+-ATPase to regulating inflammatory responses remains elusive. We report that mice haploinsufficient for the astrocyte-enriched α2Na+/K+-ATPase isoform (α2+/G301R mice) have a reduced proinflammatory response to LPS, accompanied by a reduced hypothermic reaction compared to wild type litter mates. Following intraperitoneal injection of LPS, gene expressions of Tnf-α, Il-1β, and Il-6 was reduced in the hypothalamus and hippocampus from α2+/G301R mice compared to α2+/+ littermates. The α2+/G301R mice experienced increased expression of the gene encoding an antioxidant enzyme, NRF2, in hippocampal astrocytes. Our findings indicate that α2Na+/K+-ATPase haploinsufficiency negatively modulates LPS-induced immune responses, highlighting a rational pharmacological target for reducing LPS-induced inflammation.
Potential mechanisms underlying the association between single nucleotide polymorphism (BRAP and ALDH2) and hypertension among elderly Japanese population
Authors: Shimizu Yuji , Arima Kazuhiko , Noguchi Yuko , Kawashiri Shin-Ya , Yamanashi Hirotomo , Tamai Mami , Nagata Yasuhiro , Maeda Takahiro 
Published: August 2020

AbstractMinor allele frequency (MAF) of rs3782886 (BRAP) and rs671 (ALDH2) are reported to be inversely associated with blood pressure. Another study revealed that hematopoietic activity which is evaluated by reticulocytes could influenced on hypertension status partly by indicating activity of endothelial maintenance. Therefore, to evaluate the association between genetic factor and hypertension, influence of hematopoietic activity should be considered. A multi-faced analysis was performed in a simple general elderly population model (1,313 older Japanese aged 60–98 years). Participants were stratified by median values of reticulocytes (5.21 × 104 cells/μL for men and 4.65 × 104 cells/μL for women). Independent of known cardiovascular risk factors, MAF of rs3782886 and rs671 are significantly inversely associated with hypertension for participants with high hematopoietic activity (high reticulocytes level) (fully adjusted odds ratio (ORs) were 0.72 (0.55, 0.96) for rs3782886 and 0.72 (0.54, 0.96) for rs671) but not for low reticulocytes count (the corresponding values were 1.05 (0.79, 1.39) and 1.08 (0.81, 1.45), respectively). Hematopoietic activity evaluated by reticulocytes levels could influence on the association between single nucleotide polymorphism (rs3782886 and rs671) and hypertension. Those results were efficient tool to clarify the part of the mechanism that underlying the association between genetic factor and hypertension.
Heterologous expression of high-activity cytochrome P450 in mammalian cells
Authors: Kumondai Masaki , Hishinuma Eiji , Gutiérrez Rico Evelyn Marie , Ito Akio , Nakanishi Yuya , Saigusa Daisuke , Hirasawa Noriyasu , Hiratsuka Masahiro 
Published: August 2020

AbstractThe evaluation of Cytochrome P450 (CYP) enzymatic activity is essential to estimate drug pharmacokinetics. Numerous CYP allelic variants have been identified; the functional characterisation of these variants is required for their application in precision medicine. Results from heterologous expression systems using mammalian cells can be integrated in in vivo studies; however, other systems such as E. coli, bacteria, yeast, and baculoviruses are generally used owing to the difficulty in expressing high CYP levels in mammalian cells. Here, by optimising transfection and supplementing conditions, we developed a heterologous expression system using 293FT cells to evaluate the enzymatic activities of three CYP isoforms (CYP1A2, CYP2C9, and CYP3A4). Moreover, we established co-expression with cytochrome P450 oxidoreductase and cytochrome b5. This expression system would be a potential complementary or beneficial alternative approach for the pharmacokinetic evaluation of clinically used and developing drugs in vitro.
Activation-induced cytidine deaminase overexpression in double-hit lymphoma: potential target for novel anticancer therapy
Authors: Zhang Jingcheng , Shi Yifen , Zhao Mingzhe , Hu Huixian , Huang He 
Published: August 2020

AbstractActivation-induced cytidine deaminase (AID) is one kind of the mutant enzymes, which target regulating the immunoglobulin (Ig) gene in Burkitt’s lymphoma to initiate class switch recombination (CSR), resulting in c-Myc chromosomal translocation. However, it is not clear that whether AID induces c-Myc/IgH translocation in double-hit lymphoma (DHL) with c-Myc gene translocation. In this study, the AID in DHL tissues and classical diffuse large b-cell lymphoma (DLBCL) tissues were compared. The results suggested that AID is of important value in predicting DHL, stronger CSR of AID was observed in DHL patients, which exhibited AID overexpression and c-Myc gene translocation of DHL after CSR induction. It is concluded that AID directly induces CSR in DHL and may result in c-Myc gene translocation. Targeting AID may be a good treatment regimen for DHL.
Penicillin causes non-allergic anaphylaxis by activating the contact system
Authors: Gao Yuan , Han Yixin , Zhang Xiaoyu , Fei Qiaoling , Qi Ruijuan , Hou Rui , Cai Runlan , Peng Cheng , Qi Yun 
Published: August 2020

AbstractImmediate hypersensitivity reaction (IHR) can be divided into allergic- and non-allergic-mediated, while “anaphylaxis” is reserved for severe IHR. Clinically, true penicillin allergy is rare and most reported penicillin allergy is “spurious”. Penicillin-initiated anaphylaxis is possible to occur in skin test- and specific IgE-negative patients. The contact system is a plasma protease cascade initiated by activation of factor XII (FXII). Many agents with negative ion surface can activate FXII to drive contact system. Our data showed that penicillin significantly induced hypothermia in propranolol- or pertussis toxin-pretreated mice. It also caused a rapid and reversible drop in rat blood pressure, which did not overlap with IgE-mediated hypotension. These effects could be countered by a bradykinin-B2 receptor antagonist icatibant, and consistently, penicillin indeed increased rat plasma bradykinin. Moreover, penicillin not only directly activated contact system FXII-dependently, but also promoted bradykinin release in plasma incubated-human umbilical vein endothelial cells. In fact, besides penicillin, other beta-lactams also activated the contact system in vitro. Since the autoactivation of FXII can be affected by multiple-factors, plasma from different healthy individuals showed vastly different amidolytic activity in response to penicillin, suggesting the necessity of determining the potency of penicillin to induce individual plasma FXII activation. These results clarify that penicillin-initiated non-allergic anaphylaxis is attributed to contact system activation, which might bring more effective diagnosis options for predicting penicillin-induced fatal risk and avoiding costly and inappropriate treatment clinically.
Drug-encapsulated blend of PLGA-PEG microspheres: in vitro and in vivo study of the effects of localized/targeted drug delivery on the treatment of triple-negative breast cancer
Authors: Jusu S. M. , Obayemi J. D. , Salifu A. A. , Nwazojie C. C. , Uzonwanne V. , Odusanya O. S. , Soboyejo W. O. 
Published: August 2020

AbstractTriple-negative breast cancer (TNBC) is more aggressive and difficult to treat using conventional bulk chemotherapy that is often associated with increased toxicity and side effects. In this study, we encapsulated targeted drugs [A bacteria-synthesized anticancer drug (prodigiosin) and paclitaxel] using single solvent evaporation technique with a blend of FDA-approved poly lactic-co-glycolic acid-polyethylene glycol (PLGA_PEG) polymer microspheres. These drugs were functionalized with Luteinizing Hormone-Releasing hormone (LHRH) ligands whose receptors are shown to overexpressed on surfaces of TNBC. The physicochemical, structural, morphological and thermal properties of the drug-loaded microspheres were then characterized using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), Nuclear Magnetic Resonance Spectroscopy (NMR), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC). Results obtained from in vitro kinetics drug release at human body temperature (37 °C) and hyperthermic temperatures (41 and 44 °C) reveal a non-Fickian sustained drug release that is well-characterized by Korsmeyer-Peppas model with thermodynamically non-spontaneous release of drug. Clearly, the in vitro and in vivo drug release from conjugated drug-loaded microspheres (PLGA-PEG_PGS-LHRH, PLGA-PEG_PTX-LHRH) is shown to result in greater reductions of cell/tissue viability in the treatment of TNBC. The in vivo animal studies also showed that all the drug-loaded PLGA-PEG microspheres for the localized and targeted treatment of TNBC did not caused any noticeable toxicity and thus significantly extended the survival of the treated mice post tumor resection. The implications of this work are discussed for developing targeted drug systems to treat and prevent local recurred triple negative breast tumors after surgical resection.