bluuebunny/crossref_metadata_2025_split huggingface dataset:
Energy spectra of electrons and gamma rays produced by the electron accelerator in thunderclouds
Authors: Chilingarian Ashot , Hovsepyan Gagik , Zazyan MAry 
Published: December 2020

Abstract
We measure energy spectra of electrons and gamma rays of electromagnetic avalanches developed in the electrified atmosphere as they arrive at the earth’s surface at 3200 m height where Aragats research station is located. We compare intensities and spectra shapes of 2 thunderstorm ground enhancements (TGE) observed in June and September 2020 with simulated ones. Although, the variants of electric field strength and topology assumed in the simulations are too simplified to reproduce the rather complicated and dynamic nature of the atmospheric electric field the closeness of several measured and observed parameters allows us to confirm that the relativistic runaway electron avalanches (RREA) is the origin of TGE, and to outline most probable characteristics of the atmospheric electric field for particular observed TGE events.
Problems of Designing Transport Systems of «siberian Antrazit»
Authors: Gorbunova Natalia , Mirkushov Oleg 
Published: December 2020

Abstract
The article discusses the operating conditions of transport systems located in open pit mining of the enterprise of Siberian Anthracite JSC using «Kolyvanskij» opencast mine as an example. The most optimal route for coal supply railway lines from the open pit to the concentration plants was designed, the use of which will increase the enterprise’s productivity and reduce the risks of using vehicles. When searching for the optimal route for coal delivery from «Kolyvanskij» open-pit mine to the «Listvjanskaja-1» and «Listvjanskaja-2» enrichment plants, the method of dynamically programming the optimal route (the optimality principle of R. Bellman) was used as an algorithm. The method of dynamic programming is to determine the shortest distance between nodes (places of intersection of the proposed railway tracks), by successive solutions of which, the shortest route is revealed. Based on the obtained parameters, two designed tracks were compared and, as a recommendation for solving technological issues in the design of transport systems of a mining enterprise it was proposed to use the optimal railway from the open-cast mine to «Listvjanskaja» «2 way» enrichment plant.
DARC&nbsp;Regulates Angiogenesis by Mediating Vascular Endothelial Cell Migration
Authors: Wang Xiaolin , Bian Yongqian , Li Yuejun , Li Jing , Zhao Congying , Li Jinqing , Li Xueyong 
Published: December 2020

Abstract
Background: DARC (The Duffy antigen receptor for chemokines) is a kind of glycosylated membrane protein that binds to members of the CXC chemokine family associated with angiogenesis and has recently been reported to be implicated in diverse normal physiologic processes. This study aimed to investigate the involvement of DARC in angiogenesis, which is known to generate new capillary blood vessels from preexisting ones. Methods: HDMECs (Human dermal microvascular endothelial cells) were divided into two groups (DARC overexpression group, and control group). We used Brdu staining to detect cell proliferation, and wound healing assay to detect cell migration. Then tube formation assay were observed. Also, western blot and immunofluorescent staining were used to estimate the relationship between DARC and RhoA (Ras homolog gene family, member A). Results: HDMECs proliferation, migration, and tube formation were inhibited significantly when DARC was overexpressed intracellular. DARC impaired microfilament dynamics and intercellular connection in migrating cells, and RhoA activation underlay the effect of DARC on endothelial cell. Furthermore, DARC inhibited the formation of new capillaries in vitro. Conclusion: Our ﬁndings revealed the role of DARC in the angiogenic process and provided a novel mechanism for RhoA activation during endothelial cell migration and angiogenesis.
The LINC00504/Mir-4739/KLK4 Axis Facilitates Cell Proliferation and Suppresses Cell Apoptosis Through Triggering The Wnt/Β-Catenin Pathway In Triple-Negative Breast Cancer Cells
Authors: Wu Di , Jia Hongyao , Zhang Zhiru , Li Sijie 
Published: December 2020

Abstract
Background: Currently, long non-coding RNAs (lncRNAs) have been validated to exert critical influence on the malignant progression of triple-negative breast cancer (TNBC). LncRNA long intergenic non-protein coding RNA 504 (LINC00504) has been recently reported as a tumor facilitator in the cellular processes of several cancers. However, its function in TNBC remains unknown.Methods: CCK-8 and colony formation assays were used to detect the cell viability and proliferation in TNBC. Flow cytometry analysis was utilized to measure the cycle and apoptosis of TNBC cells. The levels of key proteins associated with cell apoptosis or the β-catenin pathway were detected through western blot analysis. The activity of the Wnt/β-catenin signaling pathway was measured by the TOP/FOP flash assay. ChIP assay was conducted to confirm the binding between LINC00504 and its transcription factor signal transducer and activator of transcription 3 (STAT3). RIP and luciferase reporter assays were used to detect and verify the interaction among LINC00504 and its downstream molecule.Results: LncRNA LINC00504 was upregulated in TNBC, and silenced LINC00504 suppressed cell proliferation and triggers cell cycle arrest at G0/G1 stage and cell apoptosis in TNBC cells. STAT3 can transcriptionally activate LINC00504 and LINC00504 served as a molecular sponge of microRNA (miR-4379). Kallikrein related peptidase 4 (KLK4) was the target gene of miR-4379 and activates the Wnt/β-catenin pathway. LINC00504 upregulated KLK4 via competitively binding with miR-4379 to activate the Wnt/β-catenin pathway in TNBC. The suppression on TNBC cell proliferation and the promotion on TNBC cell cycle arrest and apoptosis under LINC00504 knockdown were rescued by miR-4379 depletion or KLK4 overexpression.Conclusions: The LINC00504/miR-4379/KLK4 axis promotes cell growth and cell cycle progression as well as suppresses cell apoptosis through activating the Wnt/β-catenin pathway.
High-Intensity Exercise Training Protects The Brain Against Autoimmune Neuroinflammation: Regulation of Microglial Redox and Pro-Inflammatory Functions
Authors: Zaychik Yifat , Fainstein Nina , Touloumi Olga , Goldberg Yehuda , Hamdi Liel , Segal Shir , Nabat Hanan , Zoidou Sofia , Grigoriadis Nikolaos , Katz Abram , Hur Tamir Ben , Einstein Ofira 
Published: December 2020

Abstract
Background: Exercise training induces beneficial effects on neurodegenerative diseases, and specifically on multiple sclerosis (MS) and its model experimental autoimmune encephalomyelitis (EAE). However, it is unclear whether exercise training exerts direct protective effects on the central nervous system (CNS), nor are the mechanisms of neuroprotection fully understood. In this study, we investigated the direct neuroprotective effects of high-intensity continuous training (HICT) against the development of autoimmune neuroinflammation and the role of resident microglia.Methods: We used the transfer EAE model to examine the direct effects of training on the CNS. Healthy mice performed HICT by treadmill running, followed by injection of encephalitogenic proteolipid (PLP)- reactive T-cells to induce EAE. EAE severity was assessed clinically and pathologically. Brain microglia from sedentary and HICT healthy mice, as well as 5-days post EAE induction (prior to onset of disease) were analyzed ex vivo for reactive oxygen species (ROS) and nitric oxide (NO) formation, mRNA expression of M1/M2 markers and neurotrophic factors, and secretion of cytokines and chemokines. Results: Transfer of encephalitogenic T-cells into HICT mice resulted in milder EAE, compared to sedentary mice, as indicated by reduced clinical severity, attenuated T-cell and neurotoxic macrophage/microglial infiltration, and reduced loss of myelin and axons. In healthy mice, HICT reduced the number of resident microglia without affecting their profile. Isolated microglia from HICT mice after transfer of encephalitogenic T-cells exhibited reduced ROS formation and released less IL-6 and monocyte chemoattractant protein in response to PLP-stimulation.Conclusions: HICT protects the CNS against autoimmune neuroinflammation by reducing microglial-derived ROS formation, neurotoxicity and pro-inflammatory responses involved in propagation of autoimmune neuroinflammation.
Exosomal lncRNA PVT1/VEGFA Axis Promotes Colon Cancer Metastasis and Stemness by Downregulation of Tumor Suppressor miR-152-3p
Authors: Lai Shiue-Wei , Chen Ming-Yao , Hsieh Ming-Shou , Huang Ting-Yi , Yeh Chi-Tai , Lee Wei-Hwa , Cherng Yih-Giun 
Published: December 2020

Abstract
The authors have requested that this preprint be removed from Research Square.
Identification Sixteen Metabolic Genes as Potential Biomarkers for Colon Adenocarcinoma&nbsp;
Authors: Cui Binbin , Zhao Fuqiang , Liu Yanlong , Gu Xinyue , Zhang Bomiao , Song Chengxin 
Published: December 2020

Abstract
Purpose Colon adenocarcinoma (COAD) is the most common primary malignant tumor of the digestive tract. It is still important to find important markers that affect the prognosis of COAD. This research aims to identify some key prognosis-related metabolic genes (PRMG) and establish a clinical prognosis model for COAD patients. Method We used The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) to obtain gene expression profiles of COAD, and then identified differentially expressed prognostic-related metabolic genes through R language and Perl software, Through univariate Cox analysis and least absolute shrinkage and selection operator (LASSO) Cox analysis to obtain target genes, established metabolic genes prognostic models and risk scores. Through COX regression analysis, independent risk factors affecting the prognosis of COAD were analyzed, and Receiver Operating Characteristic (ROC) curve analysis of independent prognostic factors was performed and a nomogram for predicting overall survival was constructed. Perform the consistency index (C-index) test and decision curve analysis (DCA) on the nomogram, and use Gene Set Enrichment Analysis (GSEA) to identify the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway of model genes. Result We selected PRMG based on the expression of metabolic genes, and used LASSO Cox regression to construct 16 metabolic gene (SEPHS1, P4HA1, ENPP2, PTGDS, GPX3, CP, ASPA, POLR3A, PKM, POLR2D , XDH, EPHX2, ADH1B, HMGCL, GPD1L and MAOA) models. The risk score generated from our model can well predict the survival prognosis of COAD. A nomogram based on the clinicopathological characteristics and risk scores of COAD can personally predict the overall survival rate of COAD patients. Conclusion We comprehensively identified metabolic genes related to the prognosis of COAD. The risk score based on the expression of 16 metabolic genes can effectively predict the prognosis of patients with COAD.
Clinical Characteristics in Patients with Hospital-Acquired Pneumonia Caused by Extensively Drug-Resistant Acinetobacter baumannii, A Multicenter Retrospective Study
Authors: Li Yujun , Huang Xiaomei , Wang Yuyao , Pan Chuzhi , Mo Zexun , Huang Muchao , Fang Changquan , Guo Penghao , Chen Huiling , Zhao Zhuxiang , Zhao Ziwen , Wei Shuquan 
Published: December 2020

Abstract
Background Extremely drug-resistant (XDR) Acinetobacter baumannii （A. baumannii）has been of a great concern. The relationship between XDR and patient outcomes remains unclear. We investigated the clinical features, risk factors, and outcomes of Hospital-acquired pneumonia (HAP)caused by XDR A. baumannii. Methods A multicenter retrospective case-control study was performed to determine factors associated with XDR A. baumannii pneumonia from 5 teaching hospitals in Guangzhou, China. Results 76 patients were enrolled in the study. XDR A. baumannii pneumonia patients were tend to be smoker (11.9% vs 3.9%, P = 0.130) and older (76.5±11.2 vs 70.3±16.4, P = 0.007) and had more comorbid diseases including chronic obstructive pulmonary disease (COPD) (48.7% vs 21.1%, P = 0.001) and renal failure (21.1% vs 3.9%, P = 0.002) and had higher APACHE II score (65.8% vs 47.4%, P = 0.033). Invasive procedures including insertion of urinary catheter, nasogastric tube, central venous/arterial catheter, bronchoscopy and mechanical ventilation along with using β-lactam/β-lactamase inhibitor and carbapenem were also risk factors for XDR A. baumannii pneumonia. Multivariate analysis showed the APACHE II score >=20 (OR, 2.1; 95% CI: 1.1–4.1, P = 0.023), COPD (OR, 9.6; 95% CI: 2.0–45.5, P = 0.004), central venous/arterial catheter placement (OR,11.5; 95% CI: 1.1-117.8, P = 0.040), low albumin levels (OR, 1.2; 95% CI: 1.1-1.4, P = 0.001) and using β-lactam/β-lactamase inhibitor (OR,15.9; 95% CI: 2.7-94.2, P = 0.002) were independent risk factors for XDR A. baumannii pneumonia. Compared with the non-XDR A. baumannii patients, the XDR A. baumannii pneumonia increased length of mechanical ventilation (11.1±12.3 vs 5.1±5.6, P = 0.000), hospital stay (42.2±24.3 vs 34.8±18.0, P = 0.036) and ICU (Intensive Care Unit) stay (27.5±19.0 vs 20.0±20.5, P = 0.020), but it did not increase in-hospital mortality (47.4% vs 32.9%, P = 0.137). Conclusions XDR A. baumannii pneumonia was strongly related to systemic illnesses, invasive procedure, low albumin levels and the APACHE II score and increasing the length of mechanical ventilation and hospital stay. But it did not increase in-hospital mortality.
Validity of 2015 ESC Risk Assessment in Idiopathic Pulmonary Arterial Hypertension in China
Authors: Gong Su-Gang , Li Chao , Zhao Qin-Hua , Jiang Rong , Wu Wen-Hui , Luo Ci-Jun , Qiu Hong-Ling , Wang Lan , Zhang Rui 
Published: December 2020

Abstract
Background: The 2015 European pulmonary hypertension guidelines recommend a risk stratification strategy for pulmonary arterial hypertension (PAH). We aimed to investigate the validation and potential prognostic information in our patients.Methods: The risk assessment variables proposed by the PH guidelines was performed, using World Health Organization function class, 6-min walking distance, brain natriuretic peptide or its N-terminal fragment, right arterial pressure, cardiac index, mixed venous saturation, right atrium area, pericardial effusion, peak oxygen consumption and ventilatory equivalents for carbon dioxide. An abbreviated version also was applied.Results: The 392 patients were enrolled between 2009 and 2018. After a median interval of 13 months, re-evaluation assessments were available for 386 subjects. The PH guidelines risk tool may effectively discriminate three risk group and mortality (p< 0.001) both at baseline and re-evaluation. Meanwhile, its simplified risk version was valid for baseline and accurately predicted the risk of death in all risk group (p< 0.001). At the time of re-evaluation, the percentage of low risk group has an increase, but a greater proportion achieved high risk group and a lesser proportion maintained in intermediate risk group.Conclusions: The 2015 European PH guidelines and its simplified version risk stratification assessment present effective discrimination of different risk groups and accurate mortality estimates in patients with IPAH. Changes of risk proportion at re-evaluation implicated that natural treatment decisions may not be consistently with goal-oriented treatment strategy.
Restoration of Colorectal Services in the Recovery Phase of the COVID-19 Pandemic: A view from Oxford
Authors: Yeung Trevor , Merchant Julia , Chen Patrick , Smart Corinne , Ghafoor Hamira , Woodhouse Fran , James David , Symons Nicholas , Boyce Stephen , Jones Oliver , George Bruce , Lindsey Ian 
Published: December 2020

Abstract
BackgroundThe coronavirus pandemic has significantly disrupted the way we deliver healthcare worldwide. We have been flexible and creative in order to continue providing elective colorectal cancer operations and to restart services for benign cases during the recovery period of the pandemic. In this paper, we describe the impact of coronavirus on our elective services and how we have implemented new patient pathways to allow us to continue providing patient care.MethodsData on major colorectal elective resections was prospectively collected in an Enhanced Recovery After Surgery (ERAS) database. Data on the number of proctology cases and telemed appointments were collected from the hospital theatre information management system and electronic patient record system respectively. ResultsDuring the pandemic, there was a complete shift towards cancer cases, with benign services and proctology cases being placed on hold. Hospital length of stay was reduced. We implemented earlier hospital discharge and more intense telephone follow up after elective major surgery. This has not resulted in an increase in post-operative complications, nor any increase in readmission into hospital. During the recovery phase, we have introduced a higher proportion of telemed consultations, including one-stop telemed proctology clinics, resulting in straight to tests or investigations.ConclusionsWe have created a streamlined multi-disciplinary pathway to reinstate our elective colorectal services as soon as possible and to minimise potential harm caused to patients whose treatment have been delayed. We anticipate many of these changes will be permanently incorporated into our clinical practice once the pandemic is over.
Assessing longitudinal and cross-sectional effects of age on adult obesity in an Iranian population: results from a large population-based cohort study
Authors: Alhossaini Mahsa Rafiee , Kazemnejad Anoshirvan , Zayeri Farid , Sadeghi Masoumeh 
Published: December 2020

Abstract
AbstractThe risk of obesity commonly changes with age, which is a longitudinal (aging) effect. Also, individuals who enter the study of the same age have similar living conditions that may influence their obesity risk in a particular way; this is a cross-sectional effect. To assess the cross-sectional and longitudinal effects of age, using a Marginal Logistic Regression model. In the current study, we used the information of individuals who had participated in the Isfahan Cohort Study. Participants were a large group of Iranian adults over 35 years of age in 2001, who lived in the central region of Iran. Repeated measurements were obtained in 2001, 2007, and 2013. From 2001 to 2013, the percentage of obesity in men and women has raised from 13% to 18% and from 31% to 44%, respectively. Both cross-sectional and longitudinal effects of age were significantly associated with the odds ratio of obesity. There was a rise in the probability of obesity for individuals with a baseline age of 35 to 60 and a decline for the older ones. The odds of obesity had about a 2% increase (on average) with each year of aging, regardless of the baseline age. The high frequency of individuals with obesity and its fast growth has been a serious public health issue among Iranian adults aged 35-60 years, especially in women. To better understand the effect of age on obesity and identify the related factors, both cross-sectional and longitudinal effects of age should be considered.
Severe pantothenic acid deficiency induces alterations in the intestinal mucosal proteome of starter Pekin ducks
Authors: Tang Jing , Feng Yulong , Zhang Bo , Wu Yongbao , Guo Zhanbao , Liang Suyun , Zhou Zhengkui , Xie Ming , Hou Shuisheng 
Published: December 2020

Abstract
Background: Pantothenic acid deficiency (PAD) results in growth depression and intestinal hypofunction of animals. However, the underlying mechanisms remain to be established. Mucosal proteome might reflect dietary influences on physiological processes.Results: A total of 128 one-day-old Pekin ducks were divided into two groups, with 8 replicates and 8 birds per replicate. All the ducks were fed either a PAD or a pantothenic acid adequate (control, CON) diet. After a 16-day feeding period, two ducks from each replicate were sampled to measure plasma parameters, intestinal morphology, and mucosal proteome. High mortality, growth retardation, fasting hypoglycemia, reduced plasma insulin, and oxidative stress were observed in the PAD group compared to the CON group. Furthermore, PAD induced morphological alterations of the small intestine indicated by reduced villus height and villus surface area of duodenum, jejunum, and ileum. The duodenum mucosal proteome of ducks showed that 198 proteins were upregulated and 223 proteins were downregulated (> 1.5-fold change) in the PAD group compared to those in the CON group. Selected proteins were confirmed by Western blotting. Pathway analysis of these proteins exhibited the suppression of glycolysis and gluconeogenesis, fatty acid beta oxidation, tricarboxylic acid cycle, oxidative phosphorylation, oxidative stress, and intestinal absorption in the PAD group, indicating impaired energy generation and abnormal intestinal absorption. We also show that nine out of eleven proteins involved in regulation of actin cytoskeleton were upregulated by PAD, probably indicates reduced intestinal integrity.Conclusion: PAD leads to growth depression and intestinal hypofunction of ducks, which are associated with impaired energy generation, abnormal intestinal absorption, and regulation of actin cytoskeleton processes. These findings provide insights into the mechanisms of intestinal mucosa metabolic disorders due to PAD.
E-DEMOCRACY IN UKRAINE: CONDITION AND DEVELOPMENT TAKING INTO ACCOUNT EUROPEAN EXPERIENCE
Authors: Yansons Yanis 
Published: December 2020

The work analyzes the mechanism of legal support of e-democracy development in Ukraine and European Union in a part of formation and implementation of its tools. A position of central executive authorities in Ukraine in the context of e-democracy development is considered.
The Ukrainian legislative base of formation and realization of e-democracy tools have been studied, and ones, considered priority in the aspect of their legal support and implementation level, have been separated. These e-democracy tools in Ukraine include: e-consulting, e-petitions, e-appeals, participation budgets (public budgets).
The analysis of e-democracy tools, offered and implemented in the EU countries allowed to establish a series of them, needed additional attention from both state authorities of Ukraine and Ukrainian publicity. These tools include: e-voting, e-referendum, e-plebiscites, and e-election process totally.
Based on comparison analysis of using e-democracy tools in Ukraine and EU, there have been separated obstacles on the way of potential improvement of the national legal mechanism of e-democracy development as to its tools usage at the national, regional and local levels. The main ones are: imperfection of resource provision of the development legal mechanism; absence of an integral idea of the development strategy of e-democracy as an institution in the state; predominance of e-services automation development over development of tools of active involvement of citizens in participation in the public-political life of the country.
Recommendations for public authorities as to improvement of the existent legal mechanism of implementation, use and development of e-democracy tools in a part of development of a series of strategic documents that must fix the priority of e-democracy development, its resource support; comprehensive support to public initiatives as to implementation and development of e-democracy and e-participation tools and so on, have been substantiated.