bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of a food and nutrition literacy questionnaire for Chinese school-age children
Authors: Liu Tan , Su Xiao , Li Niuniu , Sun Jing , Ma Guansheng , Zhu Wenli 
Published: January 2020

Abstract
Background : This study aimed to develop and validate a Food and Nutrition Literacy Questionnaire for Chinese School-age Children (FNLQ-SC).
Methods : A comprehensive literature review and a qualitative study were initially performed to identify food and nutrition literacy dimensions and core components. A cross-sectional survey was conducted in 4359 school-age children, and the junior middle school students were used to analyze the reliability and validity (n=2452). The reliability of the questionnaire was determined by internal consistency. The construct validity was assessed by exploratory factor analysis (EFA), and the content validity was assessed by Pearson correlation coefficient.
Results : By literature review and qualitative methods, 19 core components of FNLQ-SC were developed, including five dimensions of food and nutrition knowledge, the ability of access, selection, preparing of food and healthy eating. The overall FNLQ-SC questionnaire had high internal consistency (Cronbach’s α = 0.698). The exploratory factor analysis of skill components extracted 5 factors which were included in the conceptual framework, but a little different model, and cumulative contribution of variance accounted to 50.60％. The commonality was more than 0.20 for all components. The Pearson correlation coefficients between dimensions and total questionnaire were from 0.370 to 0.877. For all 4395 students, the average FNLQ-SC score of all participants was 61.91 ± 9.22, the score of knowledge and understanding was higher than that of skill dimensions. Multiple linear regression analysis indicated not only the social demographic characteristics (girls, only child, non-resident in school, urban registered permanent residence, in a more affluent family, and cared by their parents/grandparents with a higher education level), but also the home food environment were the predictors of food and nutrition literacy in school-age children (R 2 =0.226, F =81.401, P <0.05).
Conclusion : The developed Food and Nutrition Literacy Questionnaire (FNLQ-SC) had good reliability, and it could potentially be a useful instrument for assessing food and nutrition literacy for Chinese school-age children.
Impacts of skull stripping on construction of three-dimensional T1-weighted imaging-based brain structural network in full-term neonates
Authors: Wang Geliang , Hu Yajie , Li Xianjun , Wang Miaomiao , Liu Congcong , Yang Jian , Jin Chao 
Published: January 2020

Abstract
Background: Skull stripping remains a challenge for neonatal brain MR image analysis. However, little is known about how accuracy of the skull stripping affects the neonatal brain tissue segmentation and subsequent network construction. This paper therefore aimed to clarify this issue by comparing two automatic (FSL’s Brain Extraction Tool, BET; Infant Brain Extraction and Analysis Toolbox, iBEAT) and a semiautomatic (iBEAT with manual correction) processes in constructing 3D T1-weighted imaging (T1WI)-based brain structural network. Methods: Twenty-two full-term neonates (gestational age, 37-42 weeks; boys/girls, 13/9) without abnormalities on MRI who underwent brain 3D T1WI were retrospectively recruited. Two automatic (BET and iBEAT) and a semiautomatic preprocessing (iBEAT with manual correction) workflows were separately used to perform the skull stripping. Brain tissue segmentation and volume calculation were performed by a John Hopkins atlas-based method. Sixty-four gray matter regions were selected as nodes; volume covariance network and its properties (clustering coefficient, C p ; characteristic path length, L p ; local efficiency, E local ; global efficiency, E global ) were calculated by GRETNA. Analysis of variance (ANOVA) was used to compare the differences in the calculated volumes between three workflows. Results: There were significant differences in volumes of 48 brain region between three workflows ( P <0.05). Three neonatal brain structural networks presented small-world topology. The semiautomatic workflow showed remarkably decreased C p , increased L p , decreased E local , and E global , in contrast to two automatic ones. Conclusions: Imperfect skull stripping indeed affected the accuracy of brain structural network in full-term neonates.
Regulation of TRPM8 channels by PKCβ mediates morphine-induced cold hypersensitivity.
Authors: Iftinca Mircea , Basso Lilian , Flynn Robyn , Kwok Charlie , Roland Corinne , Hassan Ahmed , Defaye Manon , Ramachandran Rithwik , Trang Tuan , Altier Christophe 
Published: January 2020

Abstract
Postoperative shivering and cold hypersensitivity are major side effects of acute and chronic opioid treatments respectively. TRPM8 is a cold and menthol-sensitive channel found in a subset of dorsal root ganglion (DRG) nociceptors. Deletion or inhibition of the TRPM8 channel was found to prevent the cold hyperalgesia induced by chronic administration of morphine. Here, we examined the mechanisms by which morphine was able to promote cold hypersensitivity in DRG neurons and transfected HEK cells. Mice daily injected with morphine for five days developed cold hyperalgesia. Treatment with morphine did not alter the expressions of cold sensitive TREK-1, TRAAK and TRPM8 in DRGs. However, TRPM8-expressing DRG neurons isolated from morphine-treated mice exhibited hyperexcitability. Sustained morphine treatment in vitro sensitized TRPM8 responsiveness to cold or menthol and reduced activation-evoked desensitization of the channel. Blocking the phospholipase C (PLC) as well as protein kinase C beta (PKCβ), but not protein kinase A (PKA) or Rho-associated protein kinase (ROCK), restored channel desensitization. Identification of two PKC phosphorylation consensus sites, S1040 and S1041, in the TRPM8 and their site-directed mutation were able to prevent the MOR-induced reduction in TRPM8 desensitization. Our results show that activation of MOR by morphine 1) promotes hyperexcitability of TRPM8-expressing neurons and 2) induces a PKCβ-mediated reduction of TRPM8 desensitization. This MOR-PKCβ dependent modulation of TRPM8 may underlie the onset of cold hyperalgesia caused by repeated administration of morphine. Our findings point to TRPM8 channel and PKCβ as important targets for opioid-induced cold hypersensitivity.
Whole-exome sequencing identified a novel mutation of SLC20A2 (c.C1849T) in a Chinese family with hereditary multiple exostoses
Authors: Li YiQiang , Lin XueMei , Zhu MingWei , Xun FuXing , Li JingChun , Yuan Zhe , Liu YanHan , Xu HongWen 
Published: January 2020

Abstract
Background: Although the main causative genes for hereditary multiple exostoses (HME) are EXT-1 and EXT-2, there are still many HME patients without EXT-1 and EXT-2 mutations. This study aimed to identify novel candidate genes for the development of HME in patients without EXT-1 and EXT-2 mutations.Methods: Whole-exome sequencing was performed in a typical Chinese HME family without EXT-1 and EXT-2 mutations, followed by a combined bioinformatics pipeline including annotation and filtering process to identify candidate variants. Candidate variants were then validated by Sanger sequencing.Results: A total of 1830 original variants were revealed to be heterozygous mutations in the three patients suffering from HME that were not present in the healthy controls. Two mutations (c.C1849T in SLC20A2 and c.G506A in LETM1) were identified as a possible causative variant for HME through a bioinformatics filtering procedure and harmful prediction. Sanger sequencing results confirmed these two mutations in all patients with HME. A mutation in SLC20A2 (c.C1849T) led to a change in amino acid (p.R617C), which may be involved in the development of HME by inducing metabolic disorders of phosphate and abnormal proliferation and differentiation in chondrocytes.Conclusions: The present study revealed two mutations [SLC20A2 (c.C1849T) and LETM1 (c.G506A) in a Chinese HME family. The mutation in SLC20A2 (c.C1849T) is more likely to be involved in the development of HME.
Clinical characteristics and prenatal diagnosis in 22 families in Henan Province of China with X-linked agammaglobulinemia (XLA) related with Bruton’s tyrosine kinase (BTK) gene mutations.
Authors: gao shanshan , Hu Shuang , Duan Huikun , Wang Li , Kong Xiangdong 
Published: January 2020

Abstract
Background: X-linked agammaglobulinaemia (XLA) is a rare immunodeficiency disease, and the main clinical symptoms is recurrent severe infections. BTK is the main disease-causing gene, and the genetic mode is X chromosome recessive inheritance. But the current mutations do not fully explain this disorder. Methods: We detected the percentage of CD19+ B cells and serum immunoglobulin (IgG, IgA, and IgM) levels by Flow cytometer and rate scatter immunoturbidimetry, and investigated the mutation profile of BTK gene through Sanger sequencing and Real- Time PCR in 22 XLA patients. Results: We described the clinical symptoms and investigated the genetic mutations in 22 XLA patients, and then identified six novel mutations of BTK gene, which included 2 missense mutations (c.23G>T and c.112T>C), 2 frameshift mutations (c.522_523insC and c.1060delA), 1 large deletion (deletion of exon 2 to 5), and 1 splice-site mutation (c.1631+2T>C). Prenatal diagnosis were performed in six families (F10, F11, F15, F18, F20 and F21). F10 was a male fetus without c.922_923delGA mutation; F15 was a male fetus without c.1631+1G>T splicing mutation; F20 was a female fetus without c.1931T>C mutation; and F21 was a male fetus without large deletion mutation. All four fetuses were less likely to become XLA patients in the future. Family 11 and Family 18 were male fetuses with c.1060delA and c.1684C>T mutations, respectively. The pregnant women in F18 chose to terminate the pregnancy, and the F11 chose to continue the pregnancy. Conclusion: It is noticeable that we confirmed the diagnosis of 22 XLA patients from 22 unrelated families and found six new pathogenic mutations. Prenatal diagnosis was performed in six families. Early genetic diagnosis and routine lifelong immunoglobulin replacement therapy can prevent and treat the infections of the XLA children and save their lives.
Comparison of overall survival on surgical resection versus transarterial chemoembolization with or without radiofrequency ablation in intermediate stage hepatocellular carcinoma: A propensity score matching analysis
Authors: Lin Chih-Wen , Chen Yaw-Sen , Lo Gin-Ho , Hsu Yao-Chun , Hsu Chia-Chang , Wu Tsung-Chin , Yeh Jen-Hao , Hsiao Pojen , Hsieh Pei-Min , Lin Hung-Yu , Shu Chih-Wen , Hung Chao-Ming 
Published: January 2020

Abstract
Background: Patients with Barcelona Clinic Liver Cancer (BCLC) stage B hepatocellular carcinoma (HCC) are recommended to undergo transcatheter arterial chemoembolization (TACE). However, TACE in combination with radiofrequency ablation (RFA) is not inferior to surgical resection (SR), and the benefits of surgical resection (SR) for BCLC stage B HCC remain unclear. Hence, this study aims to compare the impact of SR, TACE+RFA, and TACE on analyzing overall survival (OS) in BCLC stage B HCC.Methods: Overall, 428 HCC patients were included in BCLC stage B, and their clinical data and OS were recorded. OS was analyzed by the Kaplan-Meier method and Cox regression analysis. Results: One hundred forty (32.7%) patients received SR, 231 (53.9%) received TACE+RFA, and 57 (13.3%) received TACE. The OS was significantly higher in the SR group than that in the TACE+RFA group [hazard ratio (HR): 1.78; 95% confidence incidence (CI): 1.15-2.75, p=0.009]. The OS was significantly higher in the SR group than that in the TACE group (HR: 3.17; 95% CI: 2.31-4.36, p<0.0001). Moreover, the OS was significantly higher in the TACE+RFA group than that in the TACE group (HR: 1.82; 95% CI: 1.21-2.74, p=0.004). The cumulative OS rates at 1, 3 and 5 years in the SR, TACE+RFA, and TACE groups were 89.2%, 69.4% and 61.2%, 86.0%, 57.9% and 38.2%, and 69.5%, 37.0% and 15.2%, respectively. After propensity score matching, the SR group still had a higher OS than those of the TACE+RFA and TACE groups. The TACE+RFA group had a higher OS than that of the TACE group. Conclusion: The SR group had higher OS than the TACE+RFA and TACE groups in BCLC stage B HCC. Furthermore, the TACE+RFA group had higher OS than the TACE group.
The relationship between preoperative T helper cytokines in the ileal mucosa and the pathogenesis of pouchitis
Authors: Kitajima Takahito , Okita Yoshiki , Kawamura Mikio , Kondo Satoru , Toiyama Yuji , Uchida Keiichi , Kusunoki Masato 
Published: January 2020

Abstract
Background: Although the etiology of pouchitis remains unknown, inflammatory cytokines are significantly associated with the pathogenesis of pouchitis. The cytokine responses that characterize inflammatory bowel diseases (IBD) are key pathogenic components of the disease. Although cytokine profiles in the colonic mucosa have been investigated in experimental colitis models or IBD patients, cytokine profiles in the ileal mucosa at colectomy have been rarely assessed.
Aim: To assess the relationship between pouchitis and T helper (Th) cytokines in the ileal mucosa collected at the time of colectomy and pouch construction.
Methods: This retrospective study involved 68 consecutive patients from January 2004 to May 2011 who underwent ileal pouch–anal anastomosis for ulcerative colitis. Samples were obtained from the terminal ileum of resected specimens at time of total colectomy or subtotal colectomy. mRNA expression levels of Th cytokines (IFN-γ, IL-23A, IL-5, IL-13 and IL‐17A) were determined.
Results: Forty of 68 patients (58.8%) developed pouchitis. There was no association between IL-23A expression levels and incidence of pouchitis ( p= 0.301). Patients with elevated IFN-γ had a significantly higher incidence of pouchitis compared with low IFN-γ patients ( p =0.043). Univariate analysis demonstrated a total dose of prednisolone >7000 mg administered before colectomy ( p =0.04) and high IFN-γ expression ( p =0.02) were significant risk factors for pouchitis onset. In multivariate analysis, elevated IFN-γ mRNA levels were significantly associated with pouchitis onset ( p =0.03).
Conclusion: IFN-γ expression in the normal ileal mucosa at the time of colectomy may be an important factor in the pathophysiology of pouchitis.
Prevalence and Antimicrobial Resistance Profiles of Bacterial Isolates Associated with Intramammary Infections in Malaysian Dairy Herds
Authors: Ali Bashir , Zunita Z. , Jesse F.F. , Ramanoon S.Z. , Mohd-Azmi M. L. 
Published: January 2020

Abstract
Intramammary infections are costly endemic disease in dairy cows and it highly affects both the quality and quantity of milk production in addition to the animal well-being. It a major cause of considerable economic losses to the dairy farmers. This study was aimed to determine the prevalence of mastitis pathogens and antimicrobial resistance pattern of selected most prevalent pathogens. A total of 1945 quarter samples from 517 cows across 33 dairy herds were used. Isolation and identification of mastitis pathogens was carried out based on standard bacteriological procedures. Antimicrobial resistance profile was conducted by disk diffusion technique. The overall culture prevalence was 67.6% (n=503/744, CI= 64.1-70.9). The respective states’ culture prevalence were 66.4% (n=71/107, CI= 56.5-75.0), 60.3% (184/305, CI= 54.6-65.8), 58% (n=94/162, CI= 50-65.6), 100% (n=75/75, CI= 93.9-100), and 83.3% (n=79/95, CI= 73.8-89.8) for Pahang, Perak, Selangor, Negeri Sembilan and Johor respectively. The most prevalent isolates identified were non-aureus staphylococci (NAS) 39.5%, S. aureus 13.1%, K. pneumoniae 6.5%, S. agalactiae 4.8%, and S. uberis 4.3%. Resistance profile of K. pneumoniae isolates against ampicillin and Penicillin G were 70.4% and 88.9% respectively while that of Actinobacter spp against chloramphenicol and streptomycin were 71.4% and 35.7% respectively. For S. uberis isolates, 66.7% and 73.3% have shown resistance to tetracycline and streptomycin respectively. This study provides knowledge of the epidemiology of bacterial isolates associated with subclinical mastitis in Malaysia and their resistance profile. The high prevalence of these pathogens in milk and multidrug resistance observed should not be over looked.
Zirconium interferences on the detection of silver nanoparticles by single particle ICP-MS: implications on natural water analysis
Authors: Turcotte Patrice , Gagnon Christian 
Published: January 2020

Abstract
Background: The analysis of silver nanoparticle (NP-Ag) by the single particle technique with argon plasma-coupled mass spectrometry (SP-ICP-MS) is an increasingly used analytical approach. The sensitive technique, distinguishing particle size distribution, allows working at concentrations similar to those found in environmental samples. The two natural Ag isotopes 107 and 109, with abundances of 52 and 48% respectively, have similar sensitivity in ICP-MS detection. However, it is common to encounter isobaric interferences in mass spectrometry, and the element silver is not an exception, as much with the 107 isotope as 109. For both isotopes, zirconium oxides present isobaric interferences, either 91Zr16O, 90Zr16O1H for the isotope 107 and the 92Zr16O1H for the 109. Results: For surface water analysis by ICP-MS in regular technique, these interferences do not generally impact the analysis of total Ag concentrations (mainly dissolved) as they can be then simply subtracted like background signal. On the other hand, detection of NPAg was impacted by the interfering colloidal Zr. The analysis of Zr by the SP-ICP-MS technique of surface waters showed the presence of colloidal Zr, a random signal that cannot be simply subtracted from NP Ag signal. Our results show that, Zr colloids are effectively interfering with the NP-Ag assays by SP-ICP-MS technique where interferences translated into a false positive. Conclusion: The analytical issue related isobaric interferences from the naturally occurring colloidal Zr was attenuated (up to 250% in this assay) by the use of the 109 isotope in the Ag detection, limiting false positive detections and improving the reliability of NP-Ag measurements in natural waters. Therefore , more specific detection of NP Ag in surface waters that naturally contain Zr colloids can be accomplished.
In silico analysis of hub genes expressed in the renal tubulointerstitium of immunoglobulin A nephropathy
Authors: Min Pan , Zhou QionXue , Duo Li , You XiaoHan , Li ZhanYuan , Zhou ZhiHong , Zhang Ji 
Published: January 2020

Abstract
Background: IgA nephropathy (IgAN) is an autoimmune disease and common cause of chronic renal failure worldwide, but its etiology has not been fully elucidated. Tubulointerstitial fibrosis/atrophy is the most reliable histological marker associated with poor outcome of IgAN. However, few studies have focused on gene expression in the renal tubulointerstitium of IgAN.Methods: A systemic search of gene expression among the renal tubulointerstitium in Gene Expression Omnibus (GEO) datasets was performed, and eligible datasets were integrated to investigate the significant genes associated with IgAN. Differentially expressed genes (DEGs) and significant gene modules of weighted gene coexpression network analysis (WGCNA) were identified, and enrichment analysis using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases was performed for interpretation of these genes.Results: Three datasets named GSE32591, GSE35489 and GSE37463 were selected for analysis of the relationships between gene expression in the renal tubulointerstitium and IgAN. Ten genes named ALB, FN1, CCL2, DUSP1, MMP7, JUNB, CTGF, TIMP3, NEU1 and HLA-E were identified as hub genes from DEGs and the significant gene module of WGCNA. Most of these genes have been related to the significant relationships of prognosis of IgAN in previous studies, but many interactions between these genes were unclear.Conclusion: Our study provided a cluster of hub genes significantly expressed in renal tubulointerstitial IgAN that may be novel treatment targets of IgAN.
Efficacy of a mixed herbal essential oils as a treatment option for clinical endometritis in dairy cattle
Authors: Davoodian Najmeh , Kadivar Ali , Elahi Raziyeh , Esfandabadi Naser Shams , Tafti Rohollah Dehghani , Rashidzade Habib Allah , Shahrbabak Mohammd Javad Behzadi , Hafshejani Taghi Taktaz 
Published: January 2020

Abstract
Backgruond: The endometritis form of uterine infection is considered as a common reproductive disorder deleterious to the reproductive performance of dairy herds. A wide variety of treatments with controversial results have been reported for endometritis, including local or systemic administration of antibiotics or disinfectants as well as hormone application. These treatments have side effects on endometrium, and antibiotic residues are found in milk following intrauterine and systemic antibiotic therapies. Objective: The aim of this study is to evaluate the possible effects of the mixed essential oil of Satureja bachtiarica Bunge, Artemisia Aucheri Boiss and Syzygium aromaticum (L.) Merr. & L.M.Perry on treatment of clinical endometritis in dairy cattle. Materials and methods: One hundred and twenty cows with clinical endometritis were selected and randomly assigned to one of the following three groups: the HM group intrauterinely received mixed herbal essential oils, the OX group received 2.5 g oxytetracycline HCl, and the EX group received 1 g of ceftiofur sodium. Results: The cleaning and first service conception rate was significantly higher in HM group than the EX, whereas the mean open days were lower in HM than the EX group. The number of service per conception was also significantly lower in HM group than OX and EX groups. In general, reproductive performance after herbal treatment was quite comparable to chemical antibiotic therapy and even better in some other reproductive indices. Conclusion: The mixed essential oils treatment represents an effective potential alternative to postpartum therapy for cows with clinical endometritis. Key words: cattle; endometritis; Satureja; Artemisia; Syzygium
Effects of post-training administration of LY341495, as a mGluR2/3 antagonist on spatial memory deficit in rats fed with high-fat diet
Authors: Moridi Heresh , Sarihi Abdolrahman , Habibitabar Elahe , Shateri Hossein , Salehi Iraj , Komaki Alireza , Karimi Jamshid , Karimi Seyed Asaad 
Published: January 2020

Abstract
Background High-fat diets (HFDs) adversely influence glutamate metabolism and neurotransmission. The precise role of the group II metabotropic glutamate receptors (mGluR2/3) antagonist on spatial memory deficit following consumption of HFD has not yet been clarified. Therefore, in this study, we examined the effects of post-training administration of mGluR2/3 antagonism; LY341495 on spatial memory in rats fed with HFD by using Morris Water Maze (MWM) task. Intraperitoneal injection (i.p) injection of LY341495 was done 30 minutes before retention test. Results Our results showed that HFD did not have any effect on memory acquisition. There were not significant differences in escape latency and swimming distance between experimental groups (P>0.05, Two-way ANOVA). Our finding showed that consumption of an HFD leads to spatial memory impairment. There were significant differences in time spent in target zone between experimental groups [F (3, 20) = 7.031, P=0.0021, one-way ANOVA]. Also, LY341495 improved HFD-induced reference memory impairment. HFD animals treated with LY341495 spent more time in the target zone in compare with HFD animals (P= 0.0449). Conclusions Our results suggested that prolonged consumption of high-fat diet has no effects on the acquisition of spatial learning, but can impair memory retention of the adult male rats and post-training administration of LY341495 can improve HFD-induced reference memory impairment.