bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA FER1L4 promotes osteogenic differentiation of human periodontal ligament stromal cells via miR-874-3p and vascular endothelial growth factor A
Authors: Huang Yiping , Han Yineng , Guo Runzhi , Liu Hao , Li Xiaobei , Jia Lingfei , Zheng Yunfei , Li Weiran 
Published: January 2020

Abstract
Background
Periodontal ligament stromal cells (PDLSCs) are ideal cell sources for periodontal tissue repair and regeneration, but little is known about what determines their osteogenic capacity. Long non-coding RNAs (lncRNAs) are important regulatory molecules at both transcriptional and post-transcriptional levels. However, their roles in the osteogenic differentiation of PDLSCs are still largely unknown.

Methods
The expression of lncRNA Fer-1-like family member 4 (FER1L4) during the osteogenic differentiation of PDLSCs was detected by quantitative reverse transcription polymerase chain reaction. Overexpression or knockdown of FER1L4 was used to confirm its regulation of osteogenesis in PDLSCs. Alkaline phosphatase and Alizarin red S staining were used to detect mineral deposition. Dual luciferase reporter assays were used to analyze the binding of miR-874-3p to FER1L4 and vascular endothelial growth factor A (VEGFA). Bone regeneration in critical-sized calvarial defects was assessed in nude mice. New bone formation was analyzed by micro-CT, hematoxylin and eosin staining, Masson’s trichrome staining, and immunohistochemical analyses.

Results
FER1L4 levels increased gradually during consecutive osteogenic induction of PDLSCs. Overexpression of FER1L4 promoted the osteogenic differentiation of PDLSCs, as revealed by alkaline phosphatase activity, Alizarin red S staining, and the expression of osteogenic markers, whereas FER1L4 knockdown inhibited these processes. Subsequently, we identified a predicted binding site for miR-874-3p on FER1L4 and confirmed a direct interaction between them. Wild-type FER1L4 reporter activity was significantly inhibited by miR-874-3p, whereas mutant FER1L4 reporter was not affected. MiR-874-3p inhibited osteogenic differentiation and reversed the promotion of osteogenesis in PDLSCs by FER1L4. Moreover, miR-874-3p targeted VEGFA, a crucial gene in osteogenic differentiation, whereas FER1L4 upregulated the expression of VEGFA. In vivo, overexpression of FER1L4 led to more bone formation compared to the control group, as demonstrated by micro-CT and the histologic analyses.

Conclusion
FER1L4 positively regulates the osteogenic differentiation of PDLSCs via miR-874-3p and VEGFA. Our study provides a promising target for enhancing the osteogenic potential of PDLSCs and periodontal regeneration.
Long noncoding RNA HULC accelerates the growth of human liver cancer stem cells by upregulating CyclinD1 through miR675-PKM2 pathway via autophagy
Authors: Wang Chen , Jiang Xiaoxue , Li Xiaonan , Song Shuting , Meng Qiuyu , Wang Liyan , Lu Yanan , Xin Xiaoru , Pu Hu , Gui Xin , Li Tianming , Lu Dongdong 
Published: January 2020

Abstract
Background
The functions of HULC have been demonstrated in several cancers. However, its mechanism has not been elucidated in human liver cancer stem cells.

Methods
Liver cancer stem cells were isolated from Huh7 cells; gene infection and tumorigenesis test in vitro and in vivo were performed.

Results
We demonstrate that HULC promotes growth of liver cancer stem cells in vitro and in vivo. Mechanistically, HULC enhances the expression of Sirt1 dependent on miR675 and then induces the cellular autophagy through Sirt1. HULC enhances CyclinD1 and thereby increases pRB and inhibited P21 WAF1/CIP 1 via autophagy-miR675-PKM2 pathway in human liver cancer stem cells. Ultimately, our results demonstrate that CyclinD1 is required for the oncogenic functions of HULC in liver cancer stem cells.

Conclusions
It reveals the key molecular signaling pathways for HULC and provides important basic information for finding effective tumor therapeutic targets based on HULC.
Review of “The Model Thinker” by Scott Page
Authors: Niazi Muaz A. , Iantovics László Barna , Temkin Anatoly 
Published: January 2020

Book detailsScott E. PageThe Model Thinker: What You Need to Know to Make Data Work for You.Hatchett Book Group.448 pages; ISBN-10: 0465094627; ISBN-13: 978-0465094622. 18.56 USD.
Role of offset and gradient architectures of 3-D melt electrowritten scaffold on differentiation and mineralization of osteoblasts
Authors: Abbasi Naghmeh , Ivanovski Saso , Gulati Karan , Love Robert M. , Hamlet Stephen 
Published: January 2020

Abstract

Background
Cell-scaffold based therapies have the potential to offer an efficient osseous regenerative treatment and PCL has been commonly used as a scaffold, however its effectiveness is limited by poor cellular retention properties. This may be improved through a porous scaffold structure with efficient pore arrangement to increase cell entrapment. To facilitate this, melt electrowriting (MEW) has been developed as a technique able to fabricate cell-supporting scaffolds with precise micro pore sizes via predictable fibre deposition. The effect of the scaffold’s architecture on cellular gene expression however has not been fully elucidated.

Methods
The design and fabrication of three different uniform pore structures (250, 500 and 750 μm), as well as two offset scaffolds with different layout of fibres (30 and 50%) and one complex scaffold with three gradient pore sizes of 250–500 - 750 μm, was performed by using MEW. Calcium phosphate modification was applied to enhance the PCL scaffold hydrophilicity and bone inductivity prior to seeding with osteoblasts which were then maintained in culture for up to 30 days. Over this time, osteoblast cell morphology, matrix mineralisation, osteogenic gene expression and collagen production were assessed.

Results
The in vitro findings revealed that the gradient scaffold significantly increased alkaline phosphatase activity in the attached osteoblasts while matrix mineralization was higher in the 50% offset scaffolds. The expression of osteocalcin and osteopontin genes were also upregulated compared to other osteogenic genes following 30 days culture, particularly in offset and gradient scaffold structures. Immunostaining showed significant expression of osteocalcin in offset and gradient scaffold structures.

Conclusions
This study demonstrated that the heterogenous pore sizes in gradient and fibre offset PCL scaffolds prepared using MEW significantly improved the osteogenic potential of osteoblasts and hence may provide superior outcomes in bone regeneration applications.
Preoperative embolization in surgical treatment of long bone metastasis: a systematic literature review
Authors: Geraets Stijn E. W. , Bos P. Koen , van der Stok Johan 
Published: January 2020

Surgery of long bone metastases is associated with a significant risk of perioperative blood loss, which may necessitate blood transfusion. Successful embolization (> 70% obliteration of vascularity) can be achieved in 36–75% of cases. The reported rate of embolization-related complications is 0–9%. Three out of six level III evidence studies showed a reduction in perioperative blood loss and/or blood transfusion requirement after preoperative embolization of renal cell carcinoma metastasis in long bones; three out of six studies did not. One level III evidence study did not show a reduction in perioperative blood loss and/or transfusion requirement after preoperative embolization of hepatocellular carcinoma metastases in long bones. There were no studies found that support preoperative embolization of thyroid metastases or other frequent long bone metastases (e.g. mamma carcinoma, lung carcinoma, or prostate carcinoma). The clinical level of evidence of the studies found is low and randomized studies taking into account primary tumour, location of metastases and type of surgery are therefore desired. Cite this article: EFORT Open Rev 2020;5:17-25. DOI: 10.1302/2058-5241.5.190013
Single-frequency chirally coupled-core all-fiber amplifier with 100  W in a linearly polarized TEM00 mode
Authors: Hochheim Sven , Steinke Michael , Wessels Peter , de Varona Omar , Koponen Joona , Lowder Tyson , Novotny Steffen , Neumann Jörg , Kracht Dietmar 
Published: January 2020

Large mode area fibers have become indispensable in addressing the power requirements of laser sources in gravitational wave detectors. Besides high power capabilities, the system must provide an excellent beam quality and polarization. In this Letter, we present the characterization of a monolithic high-power fiber amplifier at 1064 nm, built using an ytterbium-doped chirally coupled-core fiber, which achieves an output power of 100 W in a linearly polarized

T
E
M

00

mode in an all-fiber setup.
Strain tuning of the emission axis of quantum emitters in an atomically thin semiconductor
Authors: Chakraborty Chitraleema , Mukherjee Arunabh , Moon Hyowon , Konthasinghe Kumarasiri , Qiu Liangyu , Hou Wenhui , Peña Tara , Watson Carla , Wu Stephen M. , Englund Dirk , Vamivakas Nick 
Published: January 2020

Strain engineering is a natural route to control the electronic and
optical properties of two-dimensional (2D) materials. Recently, 2D
semiconductors have also been demonstrated as an intriguing host of
strain-induced quantum-confined emitters with unique valley properties
inherited from the host semiconductor. Here, we study the continuous
and reversible tuning of the light emitted by such localized emitters
in a monolayer tungsten diselenide embedded in a van der Waals
heterostructure. Biaxial strain is applied on the emitters via strain
transfer from a lead magnesium niobate–lead titanate (PMN-PT)
piezoelectric substrate. Efficient modulation of the emission energy
of several localized emitters up to 10 meV has been demonstrated on
application of a voltage on the piezoelectric substrate. Further, we
also find that the emission axis rotates by

∼

40
∘

as the magnitude of the biaxial
strain is varied on these emitters. These results elevate the prospect
of using all electrically controlled devices where the property of the
localized emitters in a 2D host can be engineered with elastic fields
for an integrated opto-electronics and nano-photonics platform.
Brillouin wavelength-selective all-optical polarization conversion
Authors: Samaniego Diego , Vidal Borja 
Published: January 2020

The manipulation of the polarization properties of light in guided media is crucial in many classical and quantum optical systems. However, the capability of current technology to finely define the state of polarization of particular wavelengths is far from the level of maturity in amplitude control. Here, we introduce a light-by-light polarization control mechanism with wavelength selectivity based on the change of the phase retardance by means of stimulated Brillouin scattering. Experiments show that any point on the Poincaré sphere can be reached from an arbitrary input state of polarization with little variation of the signal amplitude (

<
2.5

dB

). Unlike other Brillouin processing schemes, the degradation of the noise figure is small (1.5 dB for a full

2
π

rotation). This all-optical polarization controller can forge the development of new polarization-based techniques in optical communication, laser engineering, sensing, quantum systems, and light-based probing of chemical and biological systems.
All-optical tuning of a diamond micro-disk resonator on silicon
Authors: Hill Paul , Klitis Charalambos , Guilhabert Benoit , Sorel Marc , Gu Erdan , Dawson Martin D. , Strain Michael J. 
Published: January 2020

High-quality integrated diamond photonic devices have previously been demonstrated in applications from non-linear photonics to on-chip quantum optics. However, the small sample sizes of single crystal material available, and the difficulty in tuning its optical properties, are barriers to the scaling of these technologies. Both of these issues can be addressed by integrating micrometer-scale diamond devices onto host photonic integrated circuits using a highly accurate micro-assembly method. In this work a diamond micro-disk resonator is integrated with a standard single-mode silicon-on-insulator waveguide, exhibiting an average loaded

Q

-factor of

3.1
×

10

4

across a range of spatial modes, with a maximum loaded

Q

-factor of

1.05
×

10

5

. The micrometer-scale device size and high thermal impedance of the silica interface layer allow for significant thermal loading and continuous resonant wavelength tuning across a 450 pm range using a milliwatt-level optical pump. This diamond-on-demand integration technique paves the way for tunable devices coupled across large-scale photonic circuits.
Trends in Wait Time for Colorectal Cancer Screening and Diagnosis 2013-2016
Authors: Hubers Jeffrey , Sonnenberg Amnon , Gopal Deepak , Weiss Jennifer , Holobyn Thomas , Soni Anurag 
Published: January 2020

OBJECTIVES:
Colorectal cancer (CRC) screening has increased in the United States during the past 20 years, resulting in an increased demand for colonoscopy. We tested the hypothesis that such increase resulted in longer wait times for colonoscopy and influenced CRC diagnosis.

METHODS:
A total of 36,623 consecutive colonoscopies performed at the University of Wisconsin from April 8, 2013, until December 31, 2016, were included in the analysis. Wait times for colonoscopy were stratified by consecutive 6-month periods and indications of screening/surveillance vs diagnostic colonoscopy.

RESULTS:
Despite unchanged number of endoscopists, more colonoscopies were performed in 2015–2016 than in 2013–2014 (20,897 vs 15,726, respectively, P = 0.004). The mean wait time for colonoscopy increased from 68 days in 2013–2014 to 111 days in 2015–2016 (P < 0.0001), with most change affecting screening/surveillance colonoscopy. In 170 patients with a newly diagnosed CRC, the wait time did not significantly change between 2013–2014 and 2015–2016 (21 vs 27 days, respectively, P = 0.2206).

DISCUSSION:
An increase in screening/surveillance colonoscopies resulted in a substantial rise in the number of procedures between 2013 and 2016. This increase was associated with longer wait times for screening/surveillance but not diagnostic colonoscopy. Longer wait times did not result in later CRC stage at diagnosis.
Co-cultured Bone-marrow Derived and Tendon Stem Cells: Novel Seed Cells for Bone Regeneration
Authors: Liu Yang , Yuan Chengsong , Zhou Mei , Tang Kanglai 
Published: January 2020

AbstractTendon-bone healing after injury is an unsolved problem. Several types of stem cells are used as seed cells. However, the optimal co-culture ratio of different types of cells suitable for tissue engineering as well as the stimulator for facilitating the differentiation of stem cells in tendon-bone healing is unclear. In this study, the proliferation of both bone marrow-derived stem cells (BMSCs) and tendon stem cells (TSCs) was increased at a 1:1 co-cultured ratio, and proliferation was suppressed by Tenascin C (TNC). TNC treatment can promote osteogenesis or chondrogenesis of both BMSCs and TSCs under a 1:1 co-cultured ratio. In addition, the expression level of Rho-associated kinase (ROCK) increased in the process of TNC-induced osteogenesis and decreased in the process of TNC-induced chondrogenesis. Furthermore, the level of insulin-like growth factor 1 receptor (IGF-1R) and mitogen-activated protein kinase (MEK) was upregulated during the osteogenesis and chondrogenesis of both BMSCs and TSCs after TNC treatment. Although our study was conducted in rats with no direct evaluation of the resulting cells for tendon-bone healing and regeneration, we show that the proliferation of BMSCs and TSCs was enhanced under a 1:1 co-cultured ratio. TNC has a significant impact on the proliferation and differentiation of co-cultured BMSCs and TSCs. IGF-IR, ROCK, and MEK may become involved in the process after TNC treatment.
Isolation and characterisation of the agarolytic bacterium Pseudoalteromonas ruthenica
Authors: Khalifa Ashraf , Aldayel Munira 
Published: January 2020

AbstractAgar is a polysaccharide that primarily constitutes the cell wall of red algae. It is a good source of carbon and energy for many microbes. In the present study, an agarolytic bacterium, UQAD-3, was obtained from the waters of Al-Uqair, the Arabian Gulf, Al-Ahsaa, Saudi Arabia. UQAD-3 exhibited agarolytic activity when grown on agar as the sole source of carbon and energy. The strain was identified asPseudoalteromonas ruthenicabased on comparative analysis of the 16S rRNA, with 99.6% similarity. This finding was further confirmed by phylogenetic analyses based on 16S rRNA gene sequences, which highlighted that UQAD-3 was assembled within the Pseudoalteromonas clade and constituted a monophyletic subcluster with P. ruthenica, KMM 300T. The strain was further characterised biochemically using the Biolog Gen III microtest system. UQAD-3 showed positive reactions to 16 (17%) of the 94 diverse traits assessed. Good growth was reported in 10% NaCl indicating its moderate halophilic nature. These observations indicate the agarolytic potential of the strain and opens new horizons for industrial applications in the future.
Enhanced adsorption of sulfonamide antibiotics in water by modified biochar derived from bagasse
Authors: Qin Pinzhu , Huang Dawei , Tang Rong , Gan Fangqun , Guan Ying , Lv Xiaoxiao 
Published: January 2020

AbstractIn this study, biochars derived from bagasse were prepared and their ability for the adsorption of four kinds of sulfonamide antibiotics (sulfamethoxazol, thiazole, methylpyrimidine, dimethylpyrimidine) was investigated. Results showed that the modified biochar can efficiently adsorb sulfonamides in water. The biochar obtained at 500oC and modified with 30% hydrogen peroxide was chosen as the adsorbent. Under optimum conditions, pH 4 and 35°C, great adsorption performance was exhibited in the adsorption process of the four sulfonamide antibiotics. The productivity of the modified biochar was ~ 89% compared to un-modified biochar which is ~31%. The successful preparation of biochar from bagasse indicates that it is a good way to reuse the resources. Besides the adsorption of antibiotics, the obtained material also has a great prospect in the removal of other pollutants.
Doubly-adaptive artificial compression methods for incompressible flow
Authors: Layton William , McLaughlin Michael 
Published: January 2020

AbstractThis report presents adaptive artificial compression methods in which the time-step and artificial compression parameter ε are independently adapted. The resulting algorithms are supported by analysis and numerical tests. The first and second-order methods are embedded. As a result, the computational, cognitive, and space complexities of the adaptive ε, k algorithms are negligibly greater than that of the simplest, first-order, constant ε, constant k artificial compression method.
On convergent schemes for a two-phase Oldroyd-B type model with variable polymer density
Authors: Sieber Oliver 
Published: January 2020

AbstractThe paper is concerned with a diffuse-interface model that describes two-phase flow of dilute polymeric solutions with a variable particle density. The additional stresses, which arise by elongations of the polymers caused by deformations of the fluid, are described by Kramers stress tensor. The evolution of Kramers stress tensor is modeled by an Oldroyd-B type equation that is coupled to a Navier–Stokes type equation, a Cahn–Hilliard type equation, and a parabolic equation for the particle density. We present a regularized finite element approximation of this model, prove that our scheme is energy stable and that there exist discrete solutions to it. Furthermore, in the case of equal mass densities and two space dimensions, we are able to pass to the limit rigorously as the regularization parameters and the spatial and temporal discretization parameters tend towards zero and prove that a subsequence of discrete solutions converges to a global-in-time weak solution to the unregularized coupled system. To the best of our knowledge, this is the first existence result for a two-phase flow model of viscoelastic fluids with an Oldroyd-B type equation. Additionally, we show that our finite element scheme is fully practical and we present numerical simulations.
The surface layer integral method for the modelling of the radial gravity gradient component over sea surface
Authors: Zhao D. , Gong Z. , Feng J. 
Published: January 2020

Abstract
For the modelling and determination of the Earth’s external gravity potential as well as its second-order radial derivatives in the space near sea surface, the surface layer integral method was discussed in the paper. The reasons for the applicability of the method over sea surface were discussed. From the original integral formula of disturbing potential based on the surface layer method, the expression of the radial component of the gravity gradient tensor was derived. Furthermore, an identity relation was introduced to modify the formula in order to reduce the singularity problem. Numerical experiments carried out over the marine area of China show that, the modi-fied surface layer integral method effectively improves the accuracy and reliability of the calculation of the second-order radial gradient component of the disturbing potential near sea surface.
Ein Ende der Geschichte?
Authors: Bach Olaf 
Published: January 2020

Vorspann
Der Begriff Globalisierung stellt für sich genommen ein historisches Phänomen dar. Seine Geschichte verweist auf globale oder global imaginierte gesellschaftliche Kontexte seiner Entstehung und Popularisierung. Die semantischen Strukturveränderungen seines Gebrauchs spiegeln politische und wirtschaftliche Gegensätze und Dynamiken der zweiten Hälfte des 20. Jahrhunderts. Und schließlich ist der Begriffsentwicklung eine Temporalität eigen, die sich nicht in einer ins Globale gesteigerten, nochmaligen Beschleunigung der geschichtlichen Zeit erschöpft, sondern vielmehr eine spezifische Ungeschichtlichkeit der Globalisierung als „unvollendete Tatsache“ anzeigt.
The role of background music in the experience of watching YouTube videos about death and dying
Authors: Pentaris Panagiotis , Yerosimou Maria 
Published: January 2020

YouTube is the largest video sharing site live at the moment. It has been used to communicate a vast array of information, while it allows for user-generated content. This paper will focus on YouTube videos that communicate death, and in particular will present findings from a preliminary study undertaken by the authors considering the role that background music plays in these videos.
Specifically, this study explores the experiences of the viewers of death-related YouTube videos with and without background music while it makes comparisons in relation to the impact that music has on the viewers’ emotional experiences.
We conclude that background music elicits emotions and enhances feelings of sadness and sympathy in relation to the visual content of videos while recommendations for future research are made.