bluuebunny/crossref_metadata_2025_split huggingface dataset:
First‐line pembrolizumab for non–small cell lung cancer patients with PD‐L1 ≥50% in a multicenter real‐life cohort: The PEMBREIZH study
Authors: Amrane Karim , Geier Margaux , Corre Romain , Léna Hervé , Léveiller Guillaume , Gadby Florence , Lamy Régine , Bizec Jean‐Louis , Goarant Eric , Robinet Gilles , Gouva Sylvie , Quere Gilles , Abgral Ronan , Schick Ulrike , Bernier Cyril , Chouaid Christos , Descourt Renaud 
Published: February 2020

AbstractBackgroundThe KEYNOTE‐024 trial demonstrated that pembrolizumab, a PD‐1 inhibitor, significantly improves progression‐free survival (PFS) and overall survival (OS) in selected patients with previously untreated advanced non–small cell lung cancer (NSCLC) with a PD‐L1 tumor proportion score (TPS) ≥50% and without EGFR/ALK aberrations. The main aim of this study was to report the efficacy and safety profile of pembrolizumab in real‐life conditions.MethodThis was a French retrospective multicenter longitudinal study of 108 consecutive patients with advanced NSCLC, a PD‐L1 TPS ≥50% and without EGFR/ALK aberrations who were treated by pembrolizumab, in first line. Patient data were obtained from medical files.ResultsThe main characteristics of the cohort were: median age [range] 66.7 [37‐87] years, 64.8% male, 23.1% with a performance status (PS) of 2, and 88.9% current or former smokers. Eighty‐seven percent had stage IV NSCLC at diagnosis, 9.2% untreated brain metastases at inclusion,. With a median follow‐up of 8.2 months, the median PFS was 10.1 months (95% CI, 8.8‐11.4). The objective response rate was 57.3% (complete response 2.7%, partial response 54.6%). Disease control rate was 71.1%. At 6 months, the OS rate estimated was 86.2%. Treatment‐related adverse events (AE) of grade 3 occurred in 8% of patients. There were no grade 4 or 5 AEs.ConclusionIn a real‐life cohort of advanced NSCLC patients (including PS 2 and untreated brain metastases), with PD‐L1 TPS ≥50%, pembrolizumab demonstrates similar PFS to the pivotal clinical trial.
A pediatric regimen for adolescents and young adults with Philadelphia chromosome‐negative acute lymphoblastic leukemia: Results of the ALLRE08 PETHEMA trial
Authors: Ribera Josep‐Maria , Morgades Mireia , Montesinos Pau , Tormo Mar , Martínez‐Carballeira Daniel , González‐Campos José , Gil Cristina , Barba Pere , García‐Boyero Raimundo , Coll Rosa , Pedreño María , Ribera Jordi , Mercadal Santiago , Vives Susana , Novo Andrés , Genescà Eulàlia , Hernández‐Rivas Jesús‐María , Bergua Juan , Amigo María‐Luz , Vall‐Llovera Ferran , Martínez‐Sánchez Pilar , Calbacho María , García‐Cadenas Irene , Garcia‐Guiñon Antoni , Sánchez‐Sánchez María‐José , Cervera Marta , Feliu Evarist , Orfao Alberto ,   the PETHEMA Group, Spanish Society of Hematology
Published: February 2020

AbstractBackgroundPediatric‐based or ‐inspired trials have improved the prognosis of adolescents and young adults (AYA) with Philadelphia chromosome‐negative (Ph‐neg) acute lymphoblastic leukemia (ALL).MethodsThis study reports the results of treatment of the ALLRE08 trial, a full pediatric trial for AYA aged 15‐30 years with standard‐risk (SR) ALL.ResultsFrom 2008 to 2018, 89 patients (38 adolescents [15‐18 years] and 51 young adults [YA, 19‐30 years], median age: 20 [15‐29] years) were enrolled in the ALLRE08 trial. The complete response (CR) was 95%. Twenty‐two patients were transferred to a high‐risk (HR) protocol because of poor marrow response on day 14 (n = 20) or high‐level of end‐induction minimal residual response (MRD ≥ 0.25%, n = 2). Cumulative incidence of relapse (CIR) at 5 years was 35% (95%CI: 23%‐47%), with significant differences between adolescents and YA: 13% (4%‐28%) vs 52% (34%‐67%), P = .012. No treatment‐related mortality was observed in 66/66 patients following the ALLRE08 trial vs 3/23 patients moved to a HR trial. The estimated 5‐year overall survival (OS) was 74% (95%CI: 63%‐85%), with significantly higher rates for adolescents vs YA: 87% (95%CI: 74%‐100%) vs 63% (46%‐80%), P = .021. Although CIR or OS were lower in patients who were transferred to a HR trial, the differences were not statistically significant (CIR: 34% [21%‐47%] vs 37% [14%‐61%]; OS: 78% [66%‐90%] vs 61% [31%;91%]).ConclusionA full pediatric trial is feasible and effective for AYA with Ph‐neg, SR‐ALL, with better results for adolescents than for YA. Outcome of patients with poor early response rescued with a HR trial was not significantly inferior.
Expression of p16 and HPV E4 on biopsy samples and methylation of FAM19A4 and miR124‐2 on cervical cytology samples in the classification of cervical squamous intraepithelial lesions
Authors: Leeman Annemiek , Jenkins David , del Pino Marta , Ordi Jaume , Torné Aureli , Doorbar John , Meijer Chris J. L. M. , van Kemenade Folkert J. , Quint Wim G. V. 
Published: February 2020

AbstractThe decision to treat a cervical squamous intraepithelial lesion (SIL) by loop electrosurgical excision procedure (LEEP) relies heavily on a colposcopy‐directed biopsy showing high‐grade (H)SIL. Diagnosis is often supported by p16, an immunohistochemical (IHC) biomarker of high‐risk (hr)HPV E7 gene activity. Additional potential markers include methylation of tumor suppressor genes FAM19A4/miR124‐2 in cervical cytology for advanced transforming HSIL and the IHC marker HPV E4 for productive, potentially regressing lesions. In 318 women referred for colposcopy, we investigated the relationship between staining patterns of p16 and E4 IHC in the worst biopsy, and the relation of these to FAM19A4/miR124‐2 methylation status in cytology. E4‐positive staining decreased with increasing SIL/CIN grade from 41% in LSIL to 3% in HSIL/CIN3. E4 positivity increased with grade of p16 when p16 expression was limited to the lower two third of the epithelium (r = 0.378), but fell with expression over. Loss of E4 expression in the worst lesion was associated with the methylation of FAM19A4/miR124‐2. We also examined whether these biomarkers can predict the histological outcome of the LEEP biopsy in a subgroup of 119 who underwent LEEP treatment. About 85% of women with ≥lower two third p16 staining/E4‐negative HSIL biopsies and 65% with limited p16 staining/E4‐positive HSIL biopsies had ≥HSIL in the LEEP specimen (P = .025). p16 expression in a biopsy is related both to viral production and transformation, while decreased E4 expression relates to methylation, indicating advanced HSIL. p16 expression in ≥2/3 of the epithelium and absent E4 indicate likely HSIL on a subsequent LEEP specimen.
Microsatellite instability and its associations with the clinicopathologic characteristics of diffuse large B‐cell lymphoma
Authors: Tian Tian , Li Jiwei , Xue Tian , Yu Baohua , Li Xiaoqiu , Zhou Xiaoyan 
Published: February 2020

AbstractMicrosatellite instability (MSI) has been investigated as a prognostic and predictive factor for chemotherapy in colorectal cancer and has recently been demonstrated to be predictive of the PD‐1/PD‐L1 checkpoint blockade response in various solid tumors. However, MSI status in diffuse large B‐cell lymphomas (DLBCLs) has not been thoroughly explored. This study investigated MSI status in DLBCLs and analyzed the associations between MSI and clinicopathologic characteristics and clinical outcomes. Ninety‐two cases of primary DLBCLs treated with R‐CHOP/CHOP chemotherapy between 2009 and 2017 were collected. MSI detection was performed by the Promega MSI Analysis System. The protein expression of MLH1, MSH2, MSH6, and PMS2 was detected by immunohistochemistry. The associations of MSI‐H and MSI‐L with progression‐free survival (PFS) and overall survival (OS) were assessed by COX models and Kaplan–Meier curves. The correlations of complete response (CR) after R‐CHOP/CHOP chemotherapy with MSI‐H and MSI‐L were examined by univariate and multivariate logistic regression analyses, respectively. 3 of 92 cases (3.2%) were high MSI (MSI‐H), and 9 cases (9/92, 9.8%) exhibited low MSI (MSI‐L). One case with MSI‐H showed negative expression of MSH2 and MSH6. Univariate analysis indicated that MSI‐L was correlated with poor response to R‐CHOP/CHOP chemotherapy in DLBCLs (OR, 0.178; 95% CI, 0.041‐0.776; P = .022). Multivariate analysis showed that MSI‐L was an independent predictive factor for non‐CR to R‐CHOP/CHOP chemotherapy (OR, 0.144; 95% CI, 0.027‐0.761; P = .023). Kaplan‐Meier curves showed that there was a trend that MSI‐H patients had favorable PFS (P = .36) and OS (P = .48), which did not have statistical significance and MSI‐L was not significantly correlated with PFS (P = .24) and OS (P = .52).Our study indicated that there existed MSI‐H and MSI‐L in DLBCLs. MSI‐L could be an independent predictive factor for the chemotherapy response in DLBCLs.
Management and risk factors of recurrent gestational trophoblastic neoplasia: An update from 2004 to 2017
Authors: Kong Yujia , Zong Liju , Cheng Hongyan , Jiang Fang , Wan Xirun , Feng Fengzhi , Ren Tong , Zhao Jun , Yang Junjun , Xiang Yang 
Published: February 2020

AbstractObjectiveWe investigated the clinical characteristics, treatments, and survival of patients with gestational trophoblastic neoplasia (GTN) who experienced recurrence. Factors predictive of recurrence were also investigated.MethodsPatients with GTN who recurred after completing chemotherapy at Peking Union Medical College Hospital Trophoblastic Disease Center were identified between January 2004 and December 2017. Logistic regression analysis was used to identify factors predictive of GTN recurrence.ResultsA total of 1827 patients with GTN achieved complete remission (CR) at our center, of whom 118 (6.5%) experienced recurrence during follow‐up. The recurrence rates for patients initially treated at our center and those referred to us were 2.7% and 14.6%, respectively. The majority of recurrent patients received floxuridine‐based multiagent chemotherapy (n = 64). Patients who underwent surgery achieved a significantly higher CR rate than those who did not (88.6% vs 61.1%, P = .001). Although 94.1% of recurrent patients reachieved CR, 33.3% of them recurred for a second time. The 5‐year survival rate of the entire cohort was 80.4%. An interval between antecedent pregnancy and chemotherapy >12 months (OR: 6.600, 95% CI [3.217‐13.540], P < .001), and an interval from first chemotherapy to achieving β‐human chorionic gonadotropin (β‐hCG) normalization >14 weeks (OR: 2.226, 95% CI [1.080‐4.588], P = .030) were predictors of recurrence.ConclusionsPatients with recurrent GTN are prone to recurring for a second time. Surgery plays a beneficial role in the management of recurrent GTN. An interval between antecedent pregnancy and chemotherapy >12 months, and an interval from first chemotherapy to achieving β‐hCG normalization >14 weeks were predictors of recurrence.
Reciprocal language style matching in psychotherapy research
Authors: Aafjes‐van Doorn Katie , Müller‐Frommeyer Lena 
Published: February 2020

AbstractSuccessful coordination of language, that is language style matching (LSM; Gonzales, Hancock, & Pennebaker, 2010) indicated by interdependent use of function words, might be of particular relevance to the psychotherapy process. Empirically, two previous psychotherapy studies indicate that LSM may provide a deeper understanding of the quality of the therapeutic alliance. Theoretically, this process of implicit linguistic coordination may be conceptualised as interpersonal synergy (Riley, Richardson, Shockley, & Ramenzoni, 2011). To illustrate the potential of LSM in psychotherapy research, patterns of LSM per 5 min segments and reciprocal LSM per speaking turn were assessed in a seven‐session treatment demonstration of a narcissistic client. First, LSM predicted observed frequency of ruptures but did not predict observer‐rated working alliance in the corresponding 5 min segments. Second, LSM in these 5 min segments showed a quadratic trajectory of change within session and a stable pattern between sessions. Reciprocal LSM per speaking turn showed that within the majority of this treatment, the client followed the therapist's language style; however, this influence appeared to be bidirectional during the time of a physical altercation in session five. LSM may thus provide a novel way to study some of the more elusive aspects of the therapeutic interaction.
Microtubule stabilizer epothilone B as a motor neuron differentiation agent for human endometrial stem cells
Authors: Mahmoodi Narges , Ai Jafar , Ebrahimi‐Barough Somayeh , Hassannejad Zahra , Hasanzadeh Elham , Basiri Arefeh , Vaccaro Alexander R. , Rahimi‐Movaghar Vafa 
Published: February 2020

AbstractMicrotubule‐stabilizing agents (MSAs), until now, have primarily been considered for their anti‐proliferative effects in the setting of cancer. However, recent studies have revealed that one particular MSA, epothilone B (EpoB), can promote axonal regeneration after traumatic spinal cord injuries (SCI) even in the presence of inhibitor molecules such as neurite outgrowth inhibitor‐A (Nogo‐A). On the basis of the importance of having an efficient motor neuron (MN) differentiation protocol for stem cell therapy and the attention of MSAs for SCI treatment, our study investigated the effect of EpoB on human endometrial stem cells (hEnSCs) differentiation into MN‐like cells. hEnSCs were isolated and characterized by flow cytometry. The hEnSC cell viability was evaluated by a 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. To mimic the in vivo inhibitory environment, hEnSCs were also differentiated in the presence of Nogo‐A. After 15 days of differentiation, the expressions of MN‐markers were evaluated by real‐time reverse‐transcriptase polymerase chain reaction and immunofluorescence. According to the MTT assay results, three doses (1, 5, and 10 nM) of EpoB were selected to evaluate their effect on MN‐differentiation. All selected doses can increase the efficacy of hEnSCs differentiation into MN‐like cells. In particular, the 10 nM EpoB dosage was shown to increase the axon elongation, cell alignment, and upregulation of these MN‐markers compared with other doses. EpoB can improve MN differentiation from hEnSC and potentially provide a unique route for neuronal replacement in the setting of SCI.
Catheter cryoablation of ventricular ectopy originating from his region
Authors: Rossi Andrea , Frontera Antonio , Panchetti Luca , Startari Umberto , Mirizzi Gianluca , Formichi Bruno Antonio , Piacenti Marcello 
Published: February 2020

AbstractCareful mapping, early detection of AV conduction damage and cryothermal energy availability are essential in dealing with ablation procedures at the parahisian region.
Accidental poisoning with Aconitum: Case report and review of the literature
Authors: Bonanno Giuseppe , Ippolito Mariachiara , Moscarelli Alessandra , Misseri Giovanni , Caradonna Rosaria , Accurso Giuseppe , Cortegiani Andrea , Giarratano Antonino 
Published: February 2020

AbstractAconitine intoxication by ingestion of Aconitum roots can lead to ventricular tachycardia and cardiac arrest and provides an example of the potential effect of self‐medication. Educational campaigns should be implemented to contain acute intoxications caused by herbal‐derived products.
Calcium pyrophosphate deposition disease: A case report with bilateral involvement of the temporomandibular joints and concurrence of psoriatic arthritis
Authors: Loro Lado Lako , Bjørnland Tore 
Published: February 2020

AbstractCalcium pyrophosphate dehydrate deposition (CPDD) disease very rarely affects the temporomandibular joint (TMJ). It may resemble synovial chondromatosis, chondrosarcoma, chondroblastoma, or a parotid tumor. Clinical examination, CT, and MRI are important in making the correct diagnosis. Surgical removal of CPDD is necessary with or without excision of the TMJ.
Hierarchical Transition Metal Oxide Arrays Grown on Graphene‐Based Fibers with Enhanced Interface by Thin Layer of Carbon toward Solid‐State Asymmetric Supercapacitors
Authors: Xu Ting , Yang Dongzhi , Liu Yaxin , Zhang Shiyi , Zhang Ming , Zhao Tianyu , Li Xiaofeng , Yu Zhong‐Zhen 
Published: February 2020

AbstractCarbon‐based asymmetric supercapacitors with high performance hold broad application prospects in wearable devices. Forming a smooth interface between the active transition metal oxides and carbon substrate for providing electron‐transfer channels and accommodating the volume contraction for hybrid electrodes is a key issue to be tackled. Herein, both fibrous cathode and anode are designed on the basis of conductive graphene/carbon nanotube hybrid fibers by introducing pseudocapacitive NiCo2O4 nano‐grass arrays or forming interconnected pores, respectively. For hierarchical positive fibers, a nitrogen‐doped carbon (NC) middle layer is employed to effectively regulate the electronic structural states between NiCo2O4 and fiber substrate. The cycling and rate performance of cathode are significantly improved due to the strong interfacial bonding and abundant electrochemical active sites. Moreover, an interconnected porous structure is also developed to provide both unlimited axial and radial channels to promote electrolyte ion diffusion sufficiently in the porous negative electrode. The assembled solid‐state flexible asymmetric supercapacitor delivers a high energy density of 11.2 μWh cm−2 with a power density of 472.1 μW cm−2, as well as an excellent long cycling performance with 93 % capacitance retention after 10000 cycles.
Synergistic Effect of Molybdenum and Tungsten in Highly Mixed Carbide Nanoparticles as Effective Catalysts in the Hydrogen Evolution Reaction under Alkaline and Acidic Conditions
Authors: Fu Qi , Peng Baoxiang , Masa Justus , Chen Yen‐Ting , Xia Wei , Schuhmann Wolfgang , Muhler Martin 
Published: February 2020

AbstractMonometallic Mo and W carbides as well as highly mixed (Mo,W) carbides with various Mo/W ratios were synthesized directly on oxygen‐functionalized carbon nanotubes (OCNTs), and used as noble‐metal‐free electrocatalysts in the hydrogen evolution reaction (HER) under both acidic and alkaline conditions. A purely orthorhombic structure was found in both monometallic and mixed carbide samples by X‐ray diffraction. Transmission electron microscopy images showed that the carbide particles were highly dispersed on the OCNTs with well‐controlled particle size. The homogeneous distribution of Mo and W in the carbides was confirmed by elemental mapping. (Mo,W)2C/OCNT with a Mo/W ratio of 3 : 1 showed the lowest overpotential to reach a current density of 10 mA/cm2 (87 mV in 0.1 M KOH and 92 mV in 0.5 M H2SO4), and the smallest Tafel slope of 34 mV/dec. Long‐term stability under both alkaline and acidic conditions was demonstrated for 24 h. Our results revealed that an optimal amount of W in the mixed carbide can significantly improve its performance in the HER following the Tafel reaction pathway, most likely due to the weakened Mo−Hads bond.
Persistent Radical Pairs between N‐Substituted Naphthalimide and Carbanion Exhibit pKa‐Dependent UV/Vis Absorption
Authors: Nie Xiancheng , Su Hao , Chen Xiaoyu , Huang Wenhuan , Huang Linkun , Chen Biao , Miao Hui , Jiang Jun , Wang Tao , Zhang Guoqing 
Published: February 2020

AbstractA new strategy was devised for estimating and screening pKa values among different carbon acids under ambient conditions by using the UV/Vis absorption spectrum of persistent radical pairs (PRPs), which are generated from an N‐substituted naphthalimide (NNI) derivative in the presence of various carbanions in organic solutions. The electron paramagnetic resonance (EPR) spectroscopy was used to examine the presence of radicals. Unexpectedly, it was discovered that the UV/Vis spectrum of PRPs reveals a distinct linear relationship between the PRP absorption and the pKa value of a corresponding carbon acid, which is likely due to the energy difference among different RPRs. The finding may offer organic chemists an alternative reference to conduct carbanion‐mediated reactions in various organic solutions.
Asymmetric Construction of Alkaloids by Employing a Key ω‐Transaminase Cascade
Authors: Taday Freya , Ryan James , Argent Stephen P. , Caprio Vittorio , Maciá Beatriz , O'Reilly Elaine 
Published: February 2020

AbstractAn ω‐transaminase‐triggered intramolecular aza‐Michael reaction has been employed for the preparation of cyclic β‐enaminones in good yield and excellent enantio‐ and diastereoselectivity, starting from easily accessible prochiral ketoynones and commercially available enzymes. The powerful thermodynamic driving force associated with the spontaneous aza‐Michael reaction effectively displaces the transaminase reaction equilibrium towards product formation, using only two equivalents of isopropylamine. To demonstrate the potential of this methodology, this biocatalytic aza‐Michael step was combined with annulation chemistry, affording unique stereo‐defined fused alkaloid architectures.
Cr (VI) adsorption from aqueous solutions on grafted chitosan
Authors: Sanchez Ramirez Diego O. , Periolatto Monica , Carletto Riccardo A. , Varesano Alessio , Vineis Claudia , Tonetti Cinzia , Bongiovanni Roberta 
Published: February 2020

AbstractChitosan was grafted on the surface of a cotton gauze (20, 50, and 100 mg chitosan g−1 cotton) to improve its stability in aqueous solutions. The adsorption of hexavalent chromium ions from water on the grafted chitosan was evaluated to determine, by means of linear and nonlinear models, the kinetic and isotherm adsorption of the process. The kinetics of pseudo second‐order, pseudo first‐order, and adsorption isotherms type II were obtained, that is, a monolayer adsorption on nonporous adsorbents with physical adsorption was present. The most probable energy of adsorption corresponded to a physisorption with hydrogen bond interactions between chromium ions and ammonium groups. Moreover, three different cross‐sectional areas of hexavalent chromium ions were calculated and used to estimate the specific surface area employed by active sites to adsorb metal ions in terms of chitosan or cotton mass. Finally, the percentage of the area occupied by chromium ions on the surface was estimated by dividing the resulting specific surface area in terms of cotton mass by the specific surface area of cotton reported in literature. As a result, it was determined that the occupied area is between 6% (for 20 mg chitosan g−1 cotton)‐24% (for 100 mg chitosan g−1 cotton) from the total area of cotton.