bluuebunny/crossref_metadata_2025_split huggingface dataset:
DAGESTAN REGION BETWEEN THE TWO REVOLUTIONS OF 1917
(BALANCE OF SOCIO-POLITICAL POWERS)
Authors: Salihova Leyla B. , Ayagan Burkutbay G. 
Published: December 2020

The article discusses the distribution of socio-political powers in Dagestan region during the period of the February and October revolutions of 1917. The aim of the paper is to examine the process of formation of socio-political groups, to consider their opposition. The February events of 1917 in Petrograd led to the victory of the revolution. In this regard, a difficult situation arose in the Dagestan region: the victory led to a deterioration of the political situation, to demarcation within the opposing political forces. The paper highlights the formation of various political groups and government bodies which replaced tsarism. The study shows that the Provisional Regional Executive Committee and its district and local authorities, Councils of Soldiers and Officers (Workers) Deputies, religious communities, organizations belonging to one or another ethnic group, etc. were organized in the region. The authors point out the organization of the Provisional Regional Executive Committee, which became the body of power of the Provisional Government, to the opposition of the members of the committee itself, to the organization of Union of Allied Mountaineers of the North Caucasus and Dagestan, etc. The fact that representatives of socio-political groups took an active part in congresses and meetings held in the North Caucasus, Dagestan is noted.
Analysis of the period under study demonstrates that the political situation in Dagestan was tense, however the struggle of opposing parties was carried out within the democratic principles. It mainly manifested itself in the struggle between the socialist group and representatives of N. Gotsinsky. When writing the paper, the works of domestic researchers was used, among which the works of contemporaries of revolutionary events.
OPTIMALISASI PENCEGAHAN COVID-19 DI PERUMAHAN VISAR INDAH PRATAMA 2 CIBINONG KECAMATAN CIBINONG KABUPATEN BOGOR TAHUN 2020.
Authors: Maulina Hana , Raharyanti Fenny 
Published: December 2020

Covid-19 (coronavirus disease 2019) adalah penyakit yang disebabkan oleh jenis coronavirus baru yaitu Sars-CoV-2, yang dilaporkan pertama kali di Wuhan, provinsi Hubei, China pada 8 Desember 2019. Sampai saat ini virus corona menjadi masalah serius yang tengah dihadapi oleh berbagai negara di belahan dunia karena jumlah kasus setiap harinya yang terus meningkat. Tujuan dari kegiatan Kuliah Kerja Nyata Gagasan Tertulis Mandiri (KKN-GTM) ini adalah untuk mengoptimalisasi pencegahan Covid-19 di RT 04 RW 13 Perumahan Visar Indah Pratama 2 Cibinong Kecamatan Cibinong Kabupaten Bogor. Metode dalam pengumpulan data berdasarkan hasil kuesioner berupa pretest dan post-test. Populasinya adalah warga di RT 04 RW 13 Perumahan Visar Indah Pratama 2 Cibinong, dengan sampel sebanyak 20 responden. Berdasarkan hasil pretest dan post test dapat diambil kesimpulan bahwa nilai rata-rata dari hasil pretest pengetahuan covid-19 sebesar 95,30% sedangkan dari hasil post testnya sebesar 99,70%. Nilai rata-rata dari hasil pretest perilaku pencegahan covid-19 sebesar 87,5% sedangkan dari hasil post test sebesar 94,5%. Hal ini menunjukkan tingkat pengetahuan dan perilaku warga terhadap pencegahan covid-19 cukup meningkat, tetapi masih ada beberapa warga yang belum memahami pengetahuan tersebut dan menerapkan perilaku untuk mencegah penularannya.
O INFINITO: COMPREENSÕES QUE PERPASSAM TEORIAS, ENSINO E APRENDIZAGEM
Authors: Araujo Juscimar da Silva , Alves Giovana , Pinheiro José Milton Lopes , Flores César Oswaldo Vásquez 
Published: December 2020

Este estudo visa explicitar o que se evidencia como invariante entre teorias que pensam e definem o infinito, e como este invariante se expõe em situações de ensino e de aprendizagem. Para tanto, realiza-se um estudo bibliográfico, retomando o infinito no âmbito de diferentes perspectivas, buscando convergências com as quais se possa expor o que é comum aos discursos nelas articulados.Compreende-se que, em meio às variações formais e epistemológicas, a intuição de infinitude se mostra como um ponto de interseção entre os discursos matemático, físico e filosófico. Evidencia-se que, se inserida a perspectiva do ensino e aprendizagem, o mesmo ponto se destaca, como propostadidática para constituição de um solo perceptivo e intuitivo com o qual se avança a articulação de conceitos mais abstratos de infinito e de conteúdos matemáticos aos quais o mesmo está inserido.
A DISCIPLINA DE MATEMÁTICA NO ENSINO INDUSTRIAL (1942-1964)
Authors: Maciel Paulo Roberto Castor 
Published: December 2020

O objetivo do trabalho é caracterizar a disciplina de Matemática no Ensino Industrial no período compreendido entre 1942 e 1964. Utilizou-se a pesquisa histórica para realização balizada pela história cultural (CHARTIER, 2002), cujas fontes de pesquisa foram: legislação do ensino industrial, anais do III Congresso Brasileiro de Ensino de Matemática e seis trabalhos acadêmicos. A legislação definia que a disciplina deveria estar presente em todas as séries do primeiro ciclo e em uma série do segundo ciclo, além de existir uma dualidade relacionada com sua natureza (cultura geral e técnica). Os anais do congresso indicavam a metodologia para ensino da disciplina e um programa voltado para os cursos industriais básicos. Os trabalhos acadêmicos relacionavam a disciplina de forma aplicada, apresentando as diferenças com a disciplina no ensino secundário. Conclui-se que a Matemática deveria estar relacionada com a oficina, mas, apesar disso, manteve características da Matemática do ensino secundário.
CONSIDERAÇÕES TEÓRICO-METODOLÓGICAS SOBRE O DESENVOLVIMENTO DE PROJETOS COLABORATIVOS ENTRE PROFESSORES E PESQUISADORES NA CONSTRUÇÃO DE RECURSOS PARA A SALA DE AULA
Authors: Abar Celina Aparecida Almeida Pereira , Igliori Sonia Barbosa Camargo 
Published: December 2020

Neste artigo trazemos reflexões sobre o trabalho colaborativo entre professores e pesquisadores, com o objetivo de construir recursos para a sala de aula. Um trabalho colaborativo dessa forma é assumido por nós como um trabalho realizado “com” e não “sobre” o professor, e tem uma perspectiva de pesquisa-ação-formação. Os resultados devem trazer contribuições tanto para a evolução da pesquisa quanto da prática docente. A colaboração entre professor e pesquisador, portanto, é necessária e frutífera, mas para isso é preciso valorização dos dois tipos de conhecimentos e instrumentos de análise que os possam captar com inteireza. Isso porque, as pesquisas têm revelado que, qualquer que sejam teoria e metodologia norteadoras, o contexto da sala de aula tem que emergir para interessar ao professor e favorecer seu engajamento. A participação em pesquisa colaborativa das autoras deste artigo com professores, revelou que duas situações geram modalidades diferentes de dados que interferem na observação e análise dos resultados: intra (em seu desenvolvimento) e extra (após o desenvolvimento). E assim sendo é nossa proposta a utilização de uma estratégia de conexão de aportes teórico-metodológicos como uma forma de aperfeiçoamento no controle de variáveis, levantamento dos dados e consequente resultados mais significativos. Para tal considera-se a conexão entre dois aportes específicos: a Teoria da Atividade e a Difusão da Inovação para essas duas situações complementares.
ESTIMATIVAS NA EDUCAÇÃO DE JOVENS E ADULTOS: UM ESTUDO COM O BINGO DAS GRANDEZAS E MEDIDAS
Authors: Couto Charliel Lima , Bellemain Paula Moreira Baltar 
Published: December 2020

Este artigo apresenta uma pesquisa cujo objetivo foi averiguar possíveis contribuições do Bingo das Grandezas e Medidas para a construção e mobilização de conhecimentos ligados à habilidade de estimar por estudantes da Educação de Jovens e Adultos. O estudo empírico consistiu na observação participante da aplicação do jogo em uma escola municipal do Recife - PE durante três dias consecutivos, totalizando quatro rodadas, complementada pela realização de entrevista e questionário. Todos os encontros foram filmados e gravados. Os resultados mostram que, no caso dassituações cujo contexto era familiar a pelo menos parte da turma, os participantes estabeleceram relações entre suas experiências e as situações de estimativa propostas no jogo, debateram dificuldades a serem consideradas e partilharam com o grupo seus conhecimentos. No caso de situações menos habituais, eles desenvolveram estratégias, como: utilizar instrumentos de medidas, comparar com outras grandezas e realizar anotações em seus cadernos para registrar valores estimados. De modo geral, o jogo propiciou a mobilização, a partilha e a ampliação do repertório de conhecimentos dos estudantes da EJA em relação à estimativa.
Fcγ Receptor IIa (FCGR2A) Polymorphism Is Associated With Severe Respiratory Syncytial Virus Disease in Argentinian Infants
Authors: Holgado María Pía , Raiden Silvina , Sananez Inés , Seery Vanesa , De Lillo Leonardo , Maldonado Lucas L. , Kamenetzky Laura , Geffner Jorge , Arruvito Lourdes 
Published: December 2020

BackgroundMost patients with respiratory syncytial virus (RSV) infection requiring hospitalization have no risk factors for severe disease. Genetic variation in the receptor for the Fc portion of IgG (FcγR) determines their affinity for IgG subclasses driving innate and adaptive antiviral immunity. We investigated the relationship between FcγRIIa-H131R polymorphism and RSV disease.MethodsBlood samples were collected from 182 infants ≤24-month-old (50 uninfected, 114 RSV-infected with moderate course and 18 suffering severe disease). FcγRIIa-H131R SNP genotypic frequencies (HH, HR, RR) and anti-RSV IgG1, IgG2 and IgG3 levels were studied.ResultsGenotypic frequencies for FcγRIIa-H131R SNP were comparable between uninfected and RSV-infected infants. In contrast, we found a significant higher frequency of HH genotype in severe RSV-infected children compared to moderate patients. Among severe group, HH infants presented more factors associated to severity than HR or RR patients did. Furthermore, compared to moderate RSV-infected infants, severe patients showed higher levels of anti-RSV IgG1 and IgG3.ConclusionsWe found an association between an FcγRIIa (H131) polymorphism and severe RSV disease, which points towards a critical role for interactions between FcγRs and immune complexes in RSV pathogenesis. This genetic factor could also predict the worse outcome and identify those infants at risk during hospitalization.
Abnormal Neutrophil Transcriptional Signature May Predict Newly Diagnosed Latent Autoimmune Diabetes in Adults of South China
Authors: Xing Yixuan , Lin Qiuqiu , Tong Yue , Zhou Wenzhi , Huang Juan , Wang Yanfei , Huang Gan , Li Yanhua , Xiang Zhongyuan , Zhou Zhiguang , Li Tian , Xiao Yang 
Published: December 2020

ObjectiveLatent autoimmune diabetes in adults (LADA) is an autoimmune diabetes characterized by slowly progressive of β-cell function deterioration. Our previous finding demonstrated that neutrophil numbers and migration abilities display distinct levels in different types of diabetes, including LADA, whereas its pathological alterations in the development of LADA remain unknown. We aimed to investigate the changes in transcriptional levels of peripheral neutrophils in newly diagnosed LADA.MethodsPeripheral blood neutrophils were isolated from newly diagnosed LADA patients (n = 5) and age-and sex-matched healthy controls (n = 5). The Transcriptomic signature was determined by RNA sequencing (RNA-seq). Differentially expressed genes (DEG) were screened, followed by analyzing downstream Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Real-time polymerase chain reaction (qPCR) was applied for validation in LADA patients (n = 9) and age-and sex-matched healthy controls (n = 18), including sequencing samples.ResultsCompared with controls, 4105 DEG were screened in LADA patients, including 2661 upregulated and 1444 downregulated DEG. In GO analysis, DEG are mainly involved in leukocyte degranulation, myeloid cell differentiation, and immune response-regulating signaling. The top enriched KEGG pathways included cytokine-cytokine receptor interaction, adhesion molecule signaling, nuclear factor-κB (NF-κB) signaling and Th17 cell differentiation. Consistent with RNA-seq results, SELL, ITGA4, ITGAM, NCF4, ARHGAP3, and CLDN15 are upregulated in neutrophils by qPCR.ConclusionThe present study results provided a profile of DEG in the newly diagnosed LADA of south China. Our study reveals an abnormality in neutrophil disposition at the transcriptional level in LADA. Several essential genes may be involved in of LADA’s pathological process, which may be useful to guide prediction for LADA and further investigation into the pathogenesis for this disease.
The Construction and Analysis of Tumor-Infiltrating Immune Cells and ceRNA Networks in Bladder Cancer
Authors: Jiang Aimin , Liu Na , Bai Shuheng , Wang Jingjing , Gao Huan , Zheng Xiaoqiang , Fu Xiao , Ren Mengdi , Zhang Xiaoni , Tian Tao , Ruan Zhiping , Yao Yu , Liang Xuan 
Published: December 2020

BackgroundBladder cancer (BLCA) is the 11th most common malignancy worldwide. Although significant improvements have been made in screening, diagnosis, and precise management in recent years, the prognosis of BLCA remains bleak.ObjectivesThis study aimed to investigate the prognostic significance of tumor-infiltrating immune cells and construct ceRNA networks in BLCA patients.MethodsThe expression data of BLCA patients were obtained from The Cancer Genome Atlas (TCGA) database. A competing endogenous RNA (ceRNA) network was constructed to identify the hub genes involved in the prognosis of BLCA. The CIBERSORT algorithm was utilized to investigate the infiltration levels of 22 subsets of immune cells. Ultimately, the nomogram was generated to visualize the survival probability of each patient, with the calibration curve being performed to assess its performance. Furthermore, the Pearson correlation test was used to explore the correlation between the identified hub genes in the ceRNA network and the prognostic-related immune cells.ResultsA total of eight elements in the ceRNA network were considered as key members and correlated with the prognosis of BLCA, including ELN, SREBF1, DSC2, TTLL7, DIP2C, SATB1, hsa-miR-20a-5p, and hsa-miR-29c-3p. T cells CD8, T cells follicular helper (Tfh), and neutrophils were identified as independent prognostic factors in BLCA. The co-expression analysis showed that there was a significant correlation between the identified hub genes and immune cells.ConclusionOur results suggest that the mechanism of hsa-miR-29c-3p regulates the expression of ELN and DSC2, and the infiltration of Tfh and neutrophils might play pivotal roles in the progression of BLCA.
Immune-Related Neurological Toxicities of PD-1/PD-L1 Inhibitors in Cancer Patients: A Systematic Review and Meta-Analysis
Authors: Tian Yuan , Gao Aiqin , Wen Qing , Wang Shuyun , Zhang Shuisheng , Yang Xiaowei , Su Guohai , Sun Yuping 
Published: December 2020

BackgroundSystematic assessment of PD-1/PD-L1 inhibitor-related neurological toxicities is important for guiding anti-PD-1 and anti-PD-L1 immunotherapy. Therefore, we conducted this meta-analysis to reveal the relationship between PD-1/PD-L1 inhibitors and neurological toxicities among cancer patients.MethodsClinical trials investigating PD-1/PD-L1 inhibitors in cancer patients were identified by a systematic search of PubMed. The random-effect model was used to synthesize individual studies. Neurological toxicities, including all-grades and grades 3–5, were taken into account for the final comprehensive meta-analysis. The Newcastle Ottawa Scale (NOS) was used to assess the quality of included trials.ResultsThirty-one clinical trials containing data of neurological toxicities were included. Compared with chemotherapy, the risk of all-grade neurological toxicities caused by PD-1/PD-L1 inhibitors was much lower in terms of peripheral neuropathy [OR = 0.07, 95%CI:(0.04, 0.13)], peripheral sensory neuropathy [OR = 0.07, 95%CI(0.04, 0.12)], dysgeusia [OR = 0.26, 95%CI:(0.19, 0.35)], paraesthesia [OR = 0.23, 95%CI:(0.14, 0.36)], and polyneuropathy [OR = 0.12, 95%CI:(0.01, 0.94)]. However, for grades 3–5, the statistically significant results were only seen in peripheral neuropathy [OR = 0.15, 95%CI:(0.07, 0.34)] and peripheral sensory neuropathy [OR = 0.13, 95%CI:(0.04, 0.40)]. No statistically significant difference regarding the risk of headache, dizziness, and Guillain–Barré syndrome was found between PD-1/PD-L1 inhibitors and chemotherapy. For PD-1/PD-L1 inhibitors plus chemotherapy, the risk trends of the above-mentioned neurological toxicities, especially grades 3–5 peripheral neuropathy [OR = 1.76, 95%CI:(1.10, 2.82)] was increased compared to chemotherapy alone.ConclusionOur comprehensive analysis showed that PD-1/PD-L1 inhibitors alone exhibited lower neurological toxicities than chemotherapy. However, the risk of headache, dizziness, and Guillain–Barré syndrome was similar between PD-1/PD-L1 and chemotherapy. For PD-1/PD-L1 inhibitors plus chemotherapy, the incidence trend of neurological toxicities would be increased, especially for peripheral neuropathy of grades 3–5.
Altered Degranulation and pH of Neutrophil Phagosomes Impacts Antimicrobial Efficiency in Cystic Fibrosis
Authors: Hayes Elaine , Murphy Mark P. , Pohl Kerstin , Browne Niall , McQuillan Karen , Saw Le Er , Foley Clare , Gargoum Fatma , McElvaney Oliver J. , Hawkins Padraig , Gunaratnam Cedric , McElvaney Noel G. , Reeves Emer P. 
Published: December 2020

Studies have endeavored to understand the cause for impaired antimicrobial killing by neutrophils of people with cystic fibrosis (PWCF). The aim of this study was to focus on the bacterial phagosome. Possible alterations in degranulation of cytoplasmic granules and changes in pH were assessed. Circulating neutrophils were purified from PWCF (n = 28), PWCF receiving ivacaftor therapy (n = 10), and healthy controls (n = 28). Degranulation was assessed by Western blot analysis and flow cytometry. The pH of phagosomes was determined by use of BCECF-AM-labelledStaphylococcus aureusor SNARF labelledCandida albicans. The antibacterial effect of all treatments tested was determined by colony forming units enumeration. Bacterial killing by CF and healthy control neutrophils were found to differ (p = 0.0006). By use of flow cytometry and subcellular fractionation the kinetics of intraphagosomal degranulation were found to be significantly altered in CF phagosomes, as demonstrated by increased primary granule CD63 (p = 0.0001) and myeloperoxidase (MPO) content (p = 0.03). In contrast, decreased secondary and tertiary granule CD66b (p = 0.002) and decreased hCAP-18 and MMP-9 (p = 0.02), were observed. After 8 min phagocytosis the pH in phagosomes of neutrophils of PWCF was significantly elevated (p = 0.0001), and the percentage of viable bacteria was significantly increased compared to HC (p = 0.002). Results demonstrate that the recorded alterations in phagosomal pH generate suboptimal conditions for MPO related peroxidase, and α-defensin and azurocidine enzymatic killing ofStaphylococcus aureusandPseudomonas aeruginosa. The pattern of dysregulated MPO degranulation (p = 0.02) and prolonged phagosomal alkalinization in CF neutrophils were normalizedin vivofollowing treatment with the ion channel potentiator ivacaftor (p = 0.04). Our results confirm that alterations of circulating neutrophils from PWCF are corrected by CFTR modulator therapy, and raise a question related to possible delayed proton channel activity in CF.
A Synthetic Small Molecule F240B Decreases NLRP3 Inflammasome Activation by Autophagy Induction
Authors: Wu Chun-Hsien , Gan Chin Heng , Li Lan-Hui , Chang Jen-Che , Chen Shin-Tai , Menon Mridula P. , Cheng Shu-Meng , Yang Shih-Ping , Ho Chen-Lung , Chernikov Oleg V. , Lin Chi-Hung , Lam Yulin , Hua Kuo-Feng 
Published: December 2020

Conjugated polyenes are a class of widely occurring natural products with various biological functions. We previously identified 4-hydroxy auxarconjugatin B (4-HAB) as anti‐inflammatory agent with an IC50 of ~20 µM. In this study, we synthesized a new anti‐inflammatory 4-HAB analogue, F240B, which has an IC50 of less than 1 µM. F240B dose-dependently induced autophagy by increasing autophagic flux, LC3 speck formation and acidic vesicular organelle formation. F240B inhibited NACHT, LRR and PYD domain-containing protein 3 (NLRP3) inflammasome activation through autophagy induction. In a mechanistic study, F240B inhibited interleukin (IL)-1β (IL-1β) precursor expression, promoted degradation of NLRP3 and IL-1β, and reduced mitochondrial membrane integrity loss in an autophagy-dependent manner. Additionally, F240B inhibited apoptosis-associated speck-like protein containing a CARD (ASC) oligomerization and speck formation without affecting the interaction between NLRP3 and ASC or NIMA-related kinase 7 (NEK7) and double-stranded RNA-dependent kinase (PKR). Furthermore, F240B exerted in vivo anti-inflammatory activity by reducing the intraperitoneal influx of neutrophils and the levels of IL-1β, active caspase-1, IL-6 and monocyte chemoattractant protein-1 (MCP-1) in lavage fluids in a mouse model of uric acid crystal-induced peritonitis. In conclusion, F240B attenuated the NLRP3 inflammasome through autophagy induction and can be developed as an anti-inflammatory agent in the future.
Clinical Characteristics and Aqueous Humor Laboratory Analysis of Chinese Patients With Rubella Virus-Associated and Cytomegalovirus-Associated Fuchs Uveitis Syndrome
Authors: Kang Hao , Bao Han , Shi Yanhong , Feng Jing , Yang Weiqiang , He Yinzhang , Wang Hui , Hu Xiaofeng , Tao Yong 
Published: December 2020

Purpose: To describe and compare the clinical characteristics and laboratory analysis results of aqueous humor (AH) in fuchs uveitis syndrome (FUS) patients caused by rubella virus (RV) and cytomegalovirus (CMV).Methods: A retrospective and observation-based study was performed on 32 patients with FUS. Etiologies, clinical characteristics, ocular complications, visual prognoses, inflammatory cytokines, and virus-specific antibodies in AH were compared.Results: Among all the cases involved, 24 had RV FUS and 8 had CMV FUS. The mean age at diagnosis of FUS in the CMV group was older than that of the RV group (P = 0.031). The mean LogMAR best corrected visual acuity (BCVA) at initial presentation and at the final visit were both significantly higher in the CMV FUS group than those in the RV FUS group (P = 0.004, 0.047). The highest intraocular pressure (IOP) was significantly higher in the CMV group (P = 0.040). Consistent with elevated IOP, the CMV FUS patients were significantly more prone to developing glaucoma eventually than the RV FUS patients (P = 0.039). Vitreous opacity was found in 66.7% of the RV patients and 25.0% of the CMV patients (P = 0.038). The gender ratio, initial symptoms, presence and types of keratic precipitates, severity of anterior segment inflammation, iris lesions, and incidence of complicated cataract were similar between the two groups. There was no detectable difference of inflammatory cytokines in AH between RV FUS and CMV FUS.Conclusion: The clinical manifestations and disease prognosis vary between CMV FUS and RV FUS. However, clinical differences are always not obvious enough for differential diagnosis. The laboratory AH analysis is necessary to identify the etiology, determine the therapeutic strategies, and assess the disease prognosis.