bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fecal progesterone and estrogen metabolite monitoring for cyclicity and pregnancy in southern tamandua (Tamandua tetradactyla)
Authors: Daly‐Crews Kim , Edell R. Harrison , Metrione Lara C. 
Published: April 2020

AbstractReproductive management of the southern tamandua (Tamandua tetradactyla) should include timed introductions for breeding to minimize aggression and pregnancy monitoring. Since serial blood sampling could cause unnecessary stress, and urinary progesterone metabolites are found in very low concentrations, this study sought to validate progesterone and estradiol enzyme immunoassays for measuring fecal progesterone metabolite (FPM) and fecal estrogen metabolite (FEM) concentrations in two females. Peaks in FEM concentrations coincided with breeding and conception, were 5–6 times higher than baseline concentrations, and were followed by clear luteal phases distinguished by FPM concentrations 5–6 times higher than baseline concentrations. FPM concentrations during the first 30–53 days of gestation overlapped with luteal phase concentrations, thereafter increasing to 8–25 times higher than baseline concentrations. FEM concentrations during the first 41–44 days of gestation remained near basal values for one female, whereas concentrations were 1.8 times higher than baseline for the second. FEM concentrations became elevated for the former by 44 days of gestation and increased further for the latter after 53 days, ultimately averaging four times higher than baseline for both females. The biphasic increase in FPM and FEM concentrations, follicular and luteal phase durations (follicular: 7 ± 1 days, luteal: 25 ± 1 days), total cycle length (41 ± 1 days), and gestation (161–165 days) documented in this study were consistent with previous reports from serum and urine analyses. Monitoring FPM and FEM is a reliable noninvasive method for tracking reproductive cycles and pregnancy in southern tamandua that overcomes the challenges associated with serum or urinary hormone analysis.
CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5
Authors: Brito Luciana F. , Schultenkämper Kerstin , Passaglia Luciane M. P. , Wendisch Volker F. 
Published: April 2020

AbstractGene repression using the endonucleolytically deactivated dCas9 protein and sgRNAs (CRISPR interference or CRISPRi) is a useful approach to study gene functions. Here, we established CRISPRi in Paenibacillus sonchi genomovar Riograndensis SBR5, a plant growth promoting bacterium. CRISPRi system with sgRNAs targeting SBR5 endogenous genes spo0A, yaaT and ydjJ and plasmid-borne gfpUV was constructed and analyzed. Flow cytometry analysis revealed a significant decrease of reporter protein GFPUV signal in P. sonchi strains expressing gfpUV sgRNA in comparison with non-targeting controls. CRISPRi-based repression of chromosomal genes for regulation of sporulation spo0A and yaaT decreased sporulation and increased biofilm formation in SBR5. Repression of the sorbitol catabolic gene ydjJ revealed decreased specific activity of YdjJ in crude cell extracts and reduced biomass formation from sorbitol in growth experiments. Our work on CRISPRi-based gene repression serves as basis for gene function studies of the plant growth promoter P. sonchi SBR5. To our knowledge, the present study presents the first tool for gene repression established in Paenibacillus species.Key points• CRISPRi toward gene repression was applied for the first time in Paenibacillus.• CRISPRi of spo0A and yaaT depleted spores and increased biofilms in SBR5.• CRISPRi-based ydjJ repression decreased specific activity of sorbitol dehydrogenase.
Conserved roles of chromatin remodellers in cohesin loading onto chromatin
Authors: Muñoz Sofía , Passarelli Francesca , Uhlmann Frank 
Published: April 2020

AbstractCohesin is a conserved, ring-shaped protein complex that topologically entraps DNA. This ability makes this member of the structural maintenance of chromosomes (SMC) complex family a central hub of chromosome dynamics regulation. Besides its essential role in sister chromatid cohesion, cohesin shapes the interphase chromatin domain architecture and plays important roles in transcriptional regulation and DNA repair. Cohesin is loaded onto chromosomes at centromeres, at the promoters of highly expressed genes, as well as at DNA replication forks and sites of DNA damage. However, the features that determine these binding sites are still incompletely understood. We recently described a role of the budding yeast RSC chromatin remodeler in cohesin loading onto chromosomes. RSC has a dual function, both as a physical chromatin receptor of the Scc2/Scc4 cohesin loader complex, as well as by providing a nucleosome-free template for cohesin loading. Here, we show that the role of RSC in sister chromatid cohesion is conserved in fission yeast. We discuss what is known about the broader conservation of the contribution of chromatin remodelers to cohesin loading onto chromatin.
Analysis of motives and patient satisfaction in oncological second opinions provided by a certified university breast and gynecological cancer center
Authors: Loehberg Christian R. , Meyer Julia , Häberle Lothar , Hack Carolin C. , Jud Sebastian , Hein Alexander , Wunderle Marius , Emons Julius , Gass Paul , Fasching Peter A. , Egloffstein Sainab , Krebs Jessica , Erim Yesim , Beckmann Matthias W. , Lux Michael P. , Wasner Sonja 
Published: April 2020

Abstract
Purpose
Second opinions in oncology are becoming increasingly important in an era of more complex treatments and a growing demand for information by patients. Therefore, we analyzed their effects and influencing factors like patients’ motives, subjective extent of information and satisfaction with communications.

Methods
This prospective study evaluated second opinions for patients with breast cancer or gynecological malignancy. The patients received a questionnaire before and two months after, which inquired expectations, reasons, and satisfaction with the second opinion and the attending physicians.

Results
A total of 164 patients were included and the majority had breast cancer (75.0%). Receiving the second opinion made 89.7% feel better informed, their need for information decreased (from 75.3% to 39.2%, P < 0.0001), and satisfaction with doctor–patient communications increased (from 61.9 to 91.8%, P = 0.0002). There were various reasons for requesting a second opinion, e.g., the extremely stressful situation of a cancer diagnosis, hope for change in the treatment recommendation or dissatisfaction with the initial physicians.

Conclusions
Second opinions can lead to significantly greater patient satisfaction, meeting the need for information and leading to better management of patients in the extremely stressful situation of a cancer diagnosis. Doctor–patient communications play a key role.
The prevention of musculoskeletal complaints: long-term effect of a work-related psychosocial coaching intervention compared to physiotherapy alone—a randomized controlled trial
Authors: Becker Annette , Angerer Peter , Weber Jeannette , Müller Andreas 
Published: April 2020

Abstract
Purpose
Research shows that psychosocial factors play a significant role in the emergence of musculoskeletal complaints (MSC). The aim of this study was to determine the long-term effects on unspecific MSC by a combined physiotherapy and coaching intervention compared to physiotherapy alone. The coaching intervention focussed on enabling better strategies for coping with work stressors.

Methods
The participants of a previous randomized controlled intervention were invited to participate again in a third follow-up survey 22 months after the end of the intervention. In 2014, 65 nurses completed a 10-week personalised physiotherapy. Additionally, the intervention group (n = 33) passed five individual coaching sessions, plus an opening and closing session. 44 nurses (IG: n = 24; CG: n = 20) passed again a physical examination as well as another questionnaire assessment in 2016. The primary outcome was MSC, secondary outcomes were work ability and work-related well-being. Due to missing data, multiple imputations were conducted using the mice package in R. Data were analysed by ANOVA with two-way repeated measures, t tests for independent samples and Chi-squared tests.

Results
In respect of MSC, stronger improvement of movement in the vertebral column was observed in the IG compared to the CG. No differences between the IG and CG regarding other long-term effects were observed.

Conclusions
The results suggest that the combined intervention of work-related coaching and physiotherapy had only a marginally stronger long-term effect with respect to MSC than physiotherapy alone.
Parental effects influence life history traits and covary with an environmental cline in common frog populations
Authors: Rowiński Piotr K. , Laurila Anssi , Gotthard Karl , Sowersby Will , Lind Martin I. , Richter-Boix Alex , Eckerström-Liedholm Simon , Rogell Björn 
Published: April 2020

AbstractAcross latitudinal clines, the juvenile developmental rates of ectotherms often covary with the length of the growing season, due to life-history trade-offs imposed by the time-constrained environments. However, as the start of the growing season often varies substantially across years, adaptive parental effects on juvenile developmental rates may mediate the costs of a delayed season. By employing a meta-analysis, we tested whether larval developmental rates across a latitudinal cline of the common frog (Rana temporaria) are affected by fluctuating onsets of breeding, across years. We predicted that larval developmental rate will be inversely related to the onset of breeding, and that northern populations will be more prone to shorten their developmental rate in response to late breeding, as the costs of delayed metamorphosis should be highest in areas with a shorter growing season. We found that the larval period of both northern and southern populations responded to parental environmental conditions to a similar degree in absolute terms, but in different directions. In northern populations,a late season start correlated with decreased development time, suggesting that the evolution of parental effects aids population persistence in time-constrained environments. In southern populations, late season start correlated with increased development time, which could potentially be explained as a predator avoidance strategy. Our findings suggest that local ecological variables can induce adaptive parental effects, but responses are complex, and likely trade-off with other ecological factors.
Towards an Acoustic Simulation of a Water Drop Impacting in a Water Pool
Authors: Friedrich Jonas , Schäfer Michael 
Published: April 2020

AbstractThe sound which is produced when a water drop impacts into a water pool is a prominent example for acoustics produced by multiphase flow. In this work the feasibility of numerical methods for simulating this challenging test case is evaluated. First the multiphase flow needs to produce the correct physical mechanisms, e.g. the bubble entrapment. For this an in-house block-structured finite-volume solver with the volume-of-fluid method is used. For the curvature computation a standard finite difference method within the continuum surface force model is employed, including some necessary improvements. A high resolution in space and time is essential and therefore the method is parallelized by domain decomposition. The acoustic part is simulated with the linearized Euler equations which are valid in each phase but need to be adapted in the interface region. The results are compared with numerical and experimental data. It is shown, that the methods are suitable for simple test cases. A coupled drop impact test case corresponds with equivalent experiments until the drop detachment. The acoustic pressure shows a significant rise in the vicinity of the bubble detachment within both phases. However, an oscillation of the cavity bottom can not be observed in the multiphase neither in the acoustic outputs of the airborne signal.
Downsizing and enhancing broad learning systems by feature augmentation and residuals boosting
Authors: Xie Runshan , Wang Shitong 
Published: April 2020

AbstractRecently, a broad learning system (BLS) has been theoretically and experimentally confirmed to be an efficient incremental learning system. To get rid of deep architecture, BLS shares the same architecture and learning mechanism of the well-known functional link neural networks (FLNN), but works in broad learning way on both the randomly mapped features of original features of data and their randomly generated enhancement nodes. As such, BLS often requires a huge heap of hidden nodes to achieve the prescribed or satisfactory performance, which may inevitably cause both overwhelming storage requirement and overfitting phenomenon. In this study, a stacked architecture of broad learning systems called D&BLS is proposed to achieve enhanced performance and simultaneously downsize the system architecture. By boosting the residuals between previous and current layers and simultaneously augmenting the original input space with the outputs of the previous layer as the inputs of current layer, D&BLS stacks several lightweight BLS sub-systems to guarantee stronger feature representation capability and better classification/regression performance. Three fast incremental learning algorithms of D&BLS are also developed, without the need for the whole re-training. Experimental results on some popular datasets demonstrate the effectiveness of D&BLS in the sense of both enhanced performance and reduced system architecture.
Characterization of bio-coal briquettes blended from low quality coal and biomass waste treated by Garant® bio-activator and its application for fuel combustion
Authors: Mursito Anggoro Tri , Widodo  , Arifin Danang Nor 
Published: April 2020

AbstractExperimental research was carried out on the manufacturing of bio-coal briquettes from a blend of two different types of low-quality coal and biomass waste in the absence of coal carbonization, where the third blend of the material was fermented by adding a bio-activator solution before pressurizing the components into briquettes. The coal samples from Caringin–Garut Regency (BB–Garut) had a low calorific value and a high sulfur content (6.57 wt%), whereas the coal samples from Bayah–Lebak Regency (BB–Bayah) had a higher calorific value and a lower sulfur content (0.51 wt%). The biomass added to the coal blend is in the form of fermented cow dung (Bio–Kohe), and it had a calorific value of 4192 kcal/kg and a total sulfur content of 1.56 wt%. The main objective of this study is to determine the total decrease in the sulfur content in a blend of coal and biomass in which a fermentation process was carried out using a bio-activator for 24 h. The used bio-activator was made from Garant® (1:40) + molasses 1 wt%/vol, and its used amount was 0.2 L/kg. Also, the total sulfur content in the blend was 1.00 wt%–1.14 wt%, which fulfills the necessary quality requirements for non-carbonized bio-coal briquettes. The pyritic and sulfate content in the raw coal was dominant, and the organic sulfur, when fermented with Garant®, was found to be less in the produced bio-coal briquettes by 38%–58%.
Explanation of the Colour Change in Alexandrites
Authors: Xie Fei , Cao Yu , Ranchon Cindy , Hart Alan , Hansen Robin , Post Jeffrey E. , Whitney Coralyn W. , Dawson-Tarr Emma , Drew Alan J. , Dunstan David J. 
Published: April 2020

AbstractAlexandrites are remarkable and rare gemstones. They display an extraordinary colour change according to the ambient lighting, from emerald green in daylight to ruby red in incandescent light from tungsten lamps or candles. While this colour change has been correctly attributed to chromium impurities and their absorption band in the yellow region of the visible light spectrum, no adequate explanation of the mechanism has been given. Here, the alexandrite effect is fully explained by considering the von Kries model of the human colour constancy mechanism. This implies that our colour constancy mechanism is real (objective) and primarily attuned to correct for the colour temperature of black-body illuminants.
Biochemical evolution of dissolved organic matter during snow metamorphism across the ablation season for a glacier on the central Tibetan Plateau
Authors: Feng Lin , An Yanqing , Xu Jianzhong , Li Xiaofei , Jiang Bin , Liao Yuhong 
Published: April 2020

AbstractThe metamorphism of snow (snowmelt process) has a potential influence on chemical and physical process occurring within it. This study carried out a detailed study on the variation of dissolved organic matter (DOM) in different stages of snowmelt in a typical mountain glacier located at Tibetan Plateau through collecting four different surface snow/ice categories, i.e., fresh snow, fine firn, coarse firn, and granular ice during May to October in 2015. The dissolved organic carbon (DOC) was observed by lost 44% from fresh snow to fine firn and enriched 129% from fine firn to granular ice, reflecting the dynamic variability in DOC concentration during snow metamorphism. The absorbance properties of each snow category are positively correlated with DOC concentration. The result of excitation emission matrix fluorescence with parallel factor analysis (EEM-PARAFAC) and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) highlighted the domination of lipid- and protein-like compounds in glacial-derived DOM. The molecular composition of the DOM also exhibited a new N-containing molecular formula (CHON classes) that was enriched during snow metamorphism. This study suggests that snow metamorphism could induce a loss of DOM as well as enrich and modify the DOM.
Simulation of overland flow considering the influence of topographic depressions
Authors: Hu Lin , Bao Weimin , Shi Peng , Wang Jianjin , Lu Meixia 
Published: April 2020

AbstractThe simulation of overland flow, wherein runoff yield and concentration are influenced by topography, is fundamental to hydrological forecasting. Therefore, critically evaluating the characteristics of overland flow under the influence of topographic depressions—which are one of the most common microtopographic structures—is vital for improving current hydrological models. In this study, we developed a solution for the real-world application of overland flow simulations under the influence of depressions in hydrological models. A relative depression storage–outflow curve (RDOC) was proposed to investigate surface outflow processes. Experiments were conducted based on the variable-controlling approach using three rainfall return periods, four slopes, and four depression rates while ensuring a consistent initial soil moisture content. A homogenized RDOC was achieved based on shape analysis; it was parameterized by the outflow threshold and the reciprocal of the curve index of two outflow stages (B and D). A relative depression storage–outflow function (RDOF) was generated and a complete calculation procedure was applied within a hydrological model. Furthermore, we analyzed the hydrological responses to parameters of different hydrological factors to improve our understanding of the parameter determination of the RDOF.
Arzt-Bewertungsportale – Tipps und Tricks
Authors: Koch U. 
Published: April 2020

ZusammenfassungWenn die ärztliche Tätigkeit und alle sonstigen Eigenschaften der eigenen Behandlungseinrichtung öffentlich im Internet bewertet werden, empfinden viele Ärzte dies als unbequem, ehrenrührig oder sehen gar ein Risiko für den wirtschaftlichen Erfolg. Die Rechtsprechung in Deutschland ist eindeutig: Es gibt keine Vermeidungsstrategie. Dieser Beitrag führt einige Handlungsoptionen für Ärzte auf, die sich aktiv mit Online-Bewertungen auseinandersetzen wollen und bewertet diese Optionen anhand eigener Erfahrungen des Autors.
Color Stability in Temporization
Authors: Mann Navjot S. , Jhamb Ashu , Bajaj Shikha , Arora Rahul 
Published: April 2020

Abstract
Introduction Provisional restoration refers to a prosthesis which is designed to enhance the esthetic value, or function for a short time, following which it is replaced by using a fixed prosthesis. Color stability is very important factor to be kept in mind during selection of provisional materials in aesthetically important areas. Although there are various studies which look into the issue of stability of color of provisional restorations, the results are contradictory and confusing. This study was planned to compare the color stability of commonly used provisional restorative materials after exposing them to various kinds of discolorants.
Materials and Methods This work evaluated the effect of five common beverages, including tea, on color changes of different provisional materials: 1) Polymethyl methacrylates (DPI); 2) Bisacryl composite (ProtempTM II–3M ESPE); 3) Light polymerized composite (Revotek LC–GC). Color measurement of all specimens was compared with the original values of each specimen prior to immersion.
Results Within the limitations of this study, it was concluded that:a) There is no difference in color stability of provisional materials.b) Only food dye produced a clinically appreciable color change, whereas color change by cola and coffee cannot be appreciated by humans and that of tea can be appreciated only by trained eyes.
The interpreter as a sequential coordinator in courtroom interaction
Authors: Licoppe Christian , Veyrier Clair-Antoine 
Published: April 2020

Abstract
We present here an ethnographic study of asylum court interpreting with remote participants and video links. First, we
describe the multimodal resources interpreters have at their disposal to manage turn-taking and begin interpreting while an asylum seeker’s
answer to a question has not come yet to a recognizable completion point. We distinguish between ‘implicit’ configurations, in which a
collaborative turn transition is apparently achieved through reorientations of body and gaze, the use of discourse markers, or other
conversational strategies, like overlaps and cases where a turn transition is achieved through the use of ‘explicit’ resources such as
instructions to stop and requests to give brief answers. We show that the collaborative production of such long answers is affected by the
remote placement of the interpreter, and that recurrent trouble in the management of turn transitions between the asylum seeker and the
interpreter during extended narratives may be detrimental to the asylum seeker’s case.