bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cestocidal effects of Balanites aegyptiaca fruits’ methanolic extract on the adult Dipylidium caninum
Authors: Shalaby Hatem A. , Kandil Omnia M. , Hassan Noha M. , Helal Mohamed A. 
Published: April 2020

Abstract
Background
The persistent problem of canine infection with Dipylidium caninum, in spite of the wide accessibility of drugs proposed essentially to kill cestodes, recommends the need to discover other effective alternative therapy. The present study aimed to explore the role of Balanites aegyptiaca fruits against the adult D. caninum, and whether its methanolic extract had any effect on the tegument of adult cestode which is essential for the protective function, following 12- and 24-h incubation in vitro. The tegumental alterations were assessed by using both light and scanning electron microscopic studies.

Results
The Balanites extract exhibited cestocidal effect in vitro on D. caninum, and the tegument of the adult worm appeared to be its primary target. This finding was supported by differences in extract response depending on the exposure period. After 12-h incubation in vitro with Balanites extract, the tegumental changes concerned the scolex other than the proglottides, while by 24-h incubation, the changes in the adult cestodes concerned the whole body surface.

Conclusion
The tegumental distortion appeared to be the main changes induced by treatment with the tested extract. This alteration would obviously disrupt the protective function of the tegument and facilitate expelling of the adult cestode from the dog’s intestine.
Socioeconomic Factors Associated With Burnout Among Oncology Trainees
Authors: Ahmed Awad A. , Ramey Stephen J. , Dean Mary K. , Takita Cristiane , Schwartz David , Wilson Lynn D. , Vapiwala Neha , Thomas Charles R. , Shanafelt Tait D. , Deville Curtiland , Jagsi Reshma , Holliday Emma 
Published: April 2020

PURPOSE: Burnout in the medical workforce leads to early retirement, absenteeism, career changes, financial losses for medical institutions, and adverse outcomes for patients. Recent literature has explored burnout in different specialties of medicine. This article examines burnout among medical oncology trainees and identifies factors associated with burnout and professional dissatisfaction, including socioeconomic factors. METHODS: US medical oncology programs were sent a survey that included the Maslach Burnout Index–Human Services Survey as well as demographic, socioeconomic, and program-specific questions tailored to medical oncology fellowship. Primary binary end points included burnout, satisfaction with being a physician, and satisfaction with being a medical oncologist. Binomial logistic models determined associations between various characteristics and end points. RESULTS: Overall, 261 US fellows completed the survey. Seventy percent of international medical graduates reported no educational debt, whereas only 36% of US graduates reported no educational debt. Eighty-two percent of survey respondents reported their mother had at least a bachelor’s degree, and 87% of respondents reported their father had at least a bachelor’s degree. At least 27% of respondents had symptoms of burnout. Factors inversely associated with burnout on multivariable analysis included having a mother who graduated college (odds ratio [OR], 0.27), reporting an adequate perceived balance between work and personal life (OR, 0.22), feeling that faculty care about educational success (OR, 0.16), and being in the final year of training (OR, 0.45). Having debt ≥ $150,000 (OR, 2.14) was directly associated with burnout. CONCLUSION: Symptoms of burnout are common among medical oncology fellows and are associated with educational debt and socioeconomic factors.
Muscle Oxidative Capacity Is Reduced in Both Upper and Lower Limbs in COPD
Authors: ADAMI ALESSANDRA , CORVINO ROGERIO B. , CALMELAT ROBERT A. , PORSZASZ JANOS , CASABURI RICHARD , ROSSITER HARRY B. 
Published: April 2020

ABSTRACT

Introduction
Skeletal muscle atrophy, weakness, mitochondrial loss, and dysfunction are characteristics of chronic obstructive pulmonary disease (COPD). It remains unclear whether muscle dysfunction occurs in both upper and lower limbs, because findings are inconsistent in the few studies where upper and lower limb muscle performance properties were compared within an individual. This study determined whether muscle oxidative capacity is low in upper and lower limbs of COPD patients compared with controls.

Methods
Oxidative capacity of the forearm and medial gastrocnemius was measured using near-infrared spectroscopy to determine the muscle O2 consumption recovery rate constant (k, min−1) in 20 COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) 2/3/4, n = 7/7/6) and 20 smokers with normal spirometry (CON). Muscle k is linearly proportional to oxidative capacity. Steps per day and vector magnitude units per minute (VMU·min−1) were assessed using triaxial accelerometry. Differences between group and limb were assessed by two-way ANOVA.

Results
There was a significant main effect of group (F = 11.2, ηp
2 = 0.13, P = 0.001): k was lower in both upper and lower limb muscles in COPD (1.01 ± 0.17 and 1.05 ± 0.24 min−1) compared with CON (1.29 ± 0.49 and 1.54 ± 0.60 min−1). There was no effect on k of limb (F = 1.8, ηp
2 = 0.02, P = 0.18) or group–limb interaction (P = 0.35). (VMU·min−1) was significantly lower in COPD (−38%; P = 0.042). Steps per day did not differ between COPD (4738 ± 3194) and CON (6372 ± 2107; P = 0.286), although the difference exceeded a clinically important threshold (>600–1100 steps per day).

Conclusions
Compared with CON, muscle oxidative capacity was lower in COPD in both upper (−20%) and lower (−30%) limbs. These data suggest that mitochondrial loss in COPD is not isolated to locomotor muscles.
Palliative care knowledge and self-awareness in active and observing undergraduate nursing students after end-of-life simulation
Authors: Kirkpatrick Amanda J , Cantrell Mary Ann , Smeltzer Suzanne C 
Published: April 2020

Aim: The purpose of this quasi-experimental one-group repeated measure (pre-test/post-test) study was to determine the effect of an end-of-life (EoL) simulation-based experience (SBE) on active and observer nursing students' palliative care knowledge and self-awareness. Background: Baccalaureate nursing (BSN) graduates must demonstrate competence in EoL care; however, gaining EoL experience is challenging given constraints on nursing faculty and clinical sites. Research also is needed to determine whether similar outcomes are achieved by active and observer participants after EoL SBE. Method: Senior-level BSN students' palliative care knowledge and self-awareness of active and observer participants were measured before and after an EoL SBE. Results: Knowledge and self-awareness increased (P<0.001) post-SBE in all participants, with equivalent post-test scores (P≥0.248) for active (Mattitude=130.1; Mknowledge=80.5) and observer (Mattitude=128.3; Mknowledge=77.9) participants. Conclusion: EoL SBE is an effective method for increasing student palliative care competence. Student observers benefit as much as active participants on knowledge and self-awareness outcomes.
3D Printable Device for Automated Operant Conditioning in the Mouse
Authors: Mazziotti Raffaele , Sagona Giulia , Lupori Leonardo , Martini Virginia , Pizzorusso Tommaso 
Published: April 2020

AbstractOperant conditioning (OC) is a classical paradigm and a standard technique used in experimental psychology in which animals learn to perform an action to achieve a reward. By using this paradigm, it is possible to extract learning curves and measure accurately reaction times (RTs). Both these measurements are proxy of cognitive capabilities and can be used to evaluate the effectiveness of therapeutic interventions in mouse models of disease. Here, we describe a fully 3D printable device that is able to perform OC on freely moving mice, while performing real-time tracking of the animal position. We successfully trained six mice, showing stereotyped learning curves that are highly reproducible across mice and reaching >70% of accuracy after 2 d of conditioning. Different products for OC are commercially available, though most of them do not provide customizable features and are relatively expensive. This data demonstrate that this system is a valuable alternative to available state-of-the-art commercial devices, representing a good balance between performance, cost, and versatility in its use.
BNST GluN2D-Containing NMDA Receptors Influence Anxiety- and Depressive-like Behaviors and ModulateCell-Specific Excitatory/Inhibitory Synaptic Balance
Authors: Salimando Gregory J. , Hyun Minsuk , Boyt Kristen M. , Winder Danny G. 
Published: April 2020

Excitatory signaling mediated by NMDARs has been shown to regulate mood disorders. However, current treatments targeting NMDAR subtypes have shown limited success in treating patients, highlighting a need for alternative therapeutic targets. Here, we identify a role for GluN2D-containing NMDARs in modulating emotional behaviors and neural activity in the bed nucleus of the stria terminalis (BNST). Using a GluN2D KO mouse line (GluN2D−/−), we assessed behavioral phenotypes across tasks modeling emotional behavior. We then used a combination ofex vivoelectrophysiology andin vivofiber photometry to assess changes in BNST plasticity, cell-specific physiology, and cellular activity profiles. GluN2D−/−male mice exhibit evidence of exacerbated negative emotional behavior, and a deficit in BNST synaptic potentiation. We also found that GluN2D is functionally expressed on corticotropin-releasing factor (CRF)-positive BNST cells implicated in driving negative emotional states, and recordings in mice of both sexes revealed increased excitatory and reduced inhibitory drive onto GluN2D−/−BNST-CRF cellsex vivoand increased activityin vivo. Using a GluN2D conditional KO line (GluN2Dflx/flx) to selectively delete the subunit from the BNST, we find that BNST-GluN2Dflx/flxmale mice exhibit increased depressive-like behaviors, as well as altered NMDAR function and increased excitatory drive onto BNST-CRF neurons. Together, this study supports a role for GluN2D-NMDARs in regulating emotional behavior through their influence on excitatory signaling in a region-specific manner, and suggests that these NMDARs may serve as a novel target for selectively modulating glutamate signaling in stress-responsive structures and cell populations.SIGNIFICANCE STATEMENTExcitatory signaling mediated through NMDARs plays an important role in shaping emotional behavior; however, the receptor subtypes/brain regions through which this occurs are poorly understood. Here, we demonstrate that loss of GluN2D-containing NMDARs produces an increase in anxiety- and depressive-like behaviors in mice, deficits in BNST synaptic potentiation, and increased activity in BNST-CRF neurons known to drive negative emotional behavior. Further, we determine that deleting GluN2D in the BNST leads to increased depressive-like behaviors and increased excitatory drive onto BNST-CRF cells. Collectively, these results demonstrate a role for GluN2D-NMDARs in regulating the activity of stress-responsive structures and neuronal populations in the adult brain, suggesting them as a potential target for treating negative emotional states in mood-related disorders.
Children as Victims of Sexual Violence Committed by Parents: A Criminological Perspective
Authors: Susanto Joko , Utari Indah Sri 
Published: April 2020

As the technology progress and human civilization, crime which is happened not only involve a crime towards life and property, but also a crime towards morality that is increasing. As a social problem criminal act of sexual violence in nowadays has been done by adult or elderly, even from a father to his children, and most of the victim are women and children. Sexual violence is a violence which is happened because of sexuality issues. The subject of this journal is to know how the protection law for the young victim of sexual violence which is done by their parents. Discussion method which is used is Act Approach (The Statute Approach). Sexual violence usually is done toward their partner. This violence includes all of sexual violence types which are done by anyone to his sexual partner. Including sexual violence which is done by a husband to his wife, just because he wants to satisfy his biological lust. Criminal act of rape is worried, moreover when the victim are children which are still underage, because it will affect the developing of children’s psychology, inflict lifelong trauma, and the more pitiful is the children of sexual violence victim can be a prostitution worker. So that it ruins their future.
Digital Evidence Acquisition System on IAAS Cloud Computing Model using Live Forensic Method
Authors: Sudyana Didik , Lizarti Nora 
Published: April 2020

Cloud Computing is a technological development that has been warmly discussed in recent years and has seen significant increases in usage, especially on the IAAS Cloud Computing model. The high rate of development of IAAS Cloud Computing model is in line with the high number of crimes involving IAAS Cloud Computing model on server virtualization. When a computer crime occurs and a digital forensic investigation will be carried out to uncover the case, it raises issues related to the acquisition of digital evidence. Because the acquisition model in general, it is done only to one operating system, while in virtualization there is more than one operating system, so the acquisition technique in general cannot be used because it takes only one operating system involved crime, and cannot acquire the whole data server related privacy data in other virtual operating systems. Therefore, research to make the acquisition system of server virtualization is needed. The focus in this research is to make system acquisition in server virtualization Proxmox using the live forensic method to produce a system that can acquire virtualization without disrupting the overall data server and in accordance with the principle of digital forensics. The resulting acquisition system can be a reference for investigators to investigate the IAAS Cloud Computing model on Proxmox virtualization and facilitate the investigator's work in the use of the system because the investigator simply chooses which virtual operating system to acquire, after which the system will work on its own the acquisition.
Hepatitis B Surface Antigen Positivity Is an Independent Unfavorable Prognostic Factor in Diffuse Large B-Cell Lymphoma in the Rituximab Era
Authors: Cheng Chieh-Lung , Huang Sheng-Chuan , Chen Jia-Hong , Wei Chao-Hung , Fang Wei-Quan , Su Tung-Hung , Yuan Chang-Tsu , Liu Jia-Hau , Chuang Ming-Kai , Tien Hwei-Fang 
Published: April 2020

Abstract

Background
Patients with diffuse large B-cell lymphoma (DLBCL) with concurrent hepatitis B surface antigen (HBsAg)-positive hepatitis B virus (HBV) infection have distinct clinical features. Nevertheless, the prognostic value of HBsAg in DLBCL in the rituximab era remains unclear.

Materials and Methods
We conducted a retrospective cohort study to investigate the clinical relevance of HBsAg in immunocompetent patients with DLBCL treated with homogeneous rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone between 2002 and 2016.

Results
Among 416 analyzed patients, 98 (23.6%) were HBsAg positive. HBsAg positivity was associated with a younger age and more advanced stage at diagnosis, more frequent hepatic impairment during perichemotherapy, and a trend of higher National Comprehensive Cancer Network-International Prognostic Index (NCCN-IPI) score at diagnosis. Compared with the HBsAg-negative patients, the HBsAg-positive patients had a lower overall response rate (76.5% vs. 85.5%, p = .043), poorer 5-year overall survival (OS) rate (57.2% vs. 73.5%, p &lt; .001), and shorter 5-year progression-free survival (PFS) rate (47.2% vs. 60.7%, p = .013). Multivariate analyses showed that HBsAg positivity was an independent unfavorable prognostic indicator for OS and PFS. A scoring system incorporating HBsAg positivity, the NCCN-IPI score, and serum albumin levels proved to be useful for stratifying prognostically relevant subgroups of patients with DLBCL.

Conclusion
This study demonstrated that HBV infection is uniquely relevant to DLBCL. HBsAg might serve as a novel biomarker to improve clinical risk stratification of patients with DLBCL in areas with high prevalence of HBV infection. Further research investigating the etiopathogenesis of HBV infection in DLBCL is imperative.

Implications for Practice
A considerable disparity exists regarding the prognostic relevance of hepatitis B surface antigen (HBsAg)-positive hepatitis B virus (HBV) infection in patients with diffuse large B-cell lymphoma (DLBCL). In this large, retrospective cohort study from an area with high prevalence of HBV infection, the authors demonstrated that HBsAg was an independent unfavorable factor significantly associated with survival, highlighting its potential as a novel prognostic indicator to improve the risk stratification of patients with DLBCL in the rituximab era.
Evolution of Representative Bodies in Russia
Authors: Osavelyuk A. M. 
Published: April 2020

The paper based on the analysis of the provisions of the Constitution of the RSFSR of 1918, the Constitution of the USSR of 1936, the Constitution of the USSR of 1977 shows the basic principles of organization and activity of the Soviets as representative authorities of the Soviet State. The author has analyzed research papers, primarily research papers and studies by Prof. Ekaterina I. Kozlova explaining the main stages and features of evolution of the essence and activity of the Soviets at all levels of the State. Also, the author has examined the shortcomings of the legal status and functioning of domestic representative bodies of state power — the Soviets — during the Soviet period.The study has demonstrated that with the adoption of the Constitution of Russia in 1993 , the Russian Federation witnessed the development of a fundamentally new, democratic stage of development of representative bodies of state power, as well as local self — government, based on the principles of democracy, separation of powers, federalism, autonomy of local self — government and its representative bodies.Having analyzed Prof. Kozlova research papers, the author has come to the conclusion that even on the example of her research of one of the most important institutions of the state — representative bodies of state power and local self-government in the Russian Federation — Prof. Kozlova made an outstanding contribution to the development of the science of constitutional law of Russia.
Anterior Cruciate Ligament Retensioning—A Novel Revision Procedure Using Suture Augmentation
Authors: Shu Henry T. , Day Jonathan , Bodendorfer Blake M. , Argintar Evan H. 
Published: April 2020

Case:We report the case of a 44-year-old woman with previous anterior cruciate ligament (ACL) reconstruction 25 years ago in the right knee, who sustained a bucket-handle medial meniscus tear after pain and instability while pivoting during tennis. Magnetic resonance imaging demonstrated an intact but vertical and anterior ACL graft. A novel ACL retensioning procedure with suture augmentation (SA) was performed as the revision procedure. Excellent clinical outcomes and knee stability were obtained at both 17 and 36 months postoperatively.Conclusions:In certain cases, ACL retensioning with SA may be performed as the revision procedure for unsuccessful primary ACL reconstruction.
Mechanism of Wnt Pathway on Cartilage Differentiation of Adipose-Derived Stem cells
Authors: Dai Wanwu , Zhang Bo , Huang Zuquan , Sun Guili , Yang Yejing , Cai Min , Feng Zhe , Li Xingyan 
Published: April 2020

Abstract
Background: Adipose-derived stem cells ( ADSCs ) therapy is considered as a promising alternative to treat osteoarthritis (OA).Considerable evidence has shown that the Wnt signaling pathway is involved in chondrogenic differentiation in various stem cells.In the present study, we explored in detail the mechanism of of Wnt signaling pathway in the regulation of adipose-derived stem cell chondrogenic differentiation.Methohs: Adipose-derived stem cells were extracted from Sprague-Dawley groin area, isolated, cultured and verified. Then LiCl and DKK-1 used to induce ADSCs cartilage differentiation to detect indicators of cartilage at different stages, such as collagen type II (Collagen2a), aggrecan (Aggrecan) and Wnt pathway key proteins Sox9, β-catenin, Glycogen synthase kinase-3β (GSK-3β) expression.Subsequently, lentivirus was used as the vector, the full-length Sox9 gene was transfected into ADSCs and the expression levels of relevant indicators were detected again after stable expression.Results: In the process of inducing differentiation, the Wnt pathway promoted the rapid proliferation of ADSCs in the early, middle and late stages, and up-regulates Sox9 expression.Cartilage indicators Collagen 2a and Aggrecan expression levels were not significantly increased in the early stage, but significantly increased in the middle and late stages (P <0.05). At the later stage, however, the Wnt pathway down-regulated Sox9 expression, weakened the mature cartilage phenotype, promoted cartilage hypertrophy and early cartilage ossification. Finally, the overexpression of Sox9 feedback inhibits the Wnt pathway activity, down-regulates β-catenin expression, maintains cartilage phenotype, delays cartilage hypertrophy and early osteogenesis.Conclusion:The Wnt pathway regulates chondrogenic differentiation of ADSCs by regulating Sox9, but its regulatory mechanism is significantly different at different stages of induced differentiation.