bluuebunny/crossref_metadata_2025_split huggingface dataset:
RAGE mediates airway inflammation via the HDAC1 pathway in a toluene diisocyanate-induced murine asthma model
Authors: Peng Xianru , Huang Minyu , Zhao Wenqu , Lan Zihan , Wang Xiaohua , Yuan Yafei , Li Bohou , Yu Changhui , Liu Laiyu , Dong Hangming , Cai Shaoxi , Zhao Haijin 
Published: August 2020

Abstract
BackgroundExposure to toluene diisocyanate (TDI) is a significant pathogenic factor for asthma. We previously reported that receptor for advanced glycation end products (RAGE) plays a key role in TDI-induced asthma; however, the mechanism is not clear. Epigenetic alterations of histone deacetylase (HDAC) are associated with allergic asthma. However, its effect in TDI-induced asthma is not known. The purpose of this study was to determine the role of RAGE and HDAC1 in the regulation of airway inflammation using a TDI-induced asthma model.MethodsBALB/c mice were sensitized, and challenged by TDI to establish murine asthma models. FPS-ZM1 (RAGE inhibitor), JNJ-26482585 and romidepsin (HDAC inhibitor) were given intraperitoneally before each challenge. The human bronchial epithelial cell line 16HBE was stimulated by TDI-human serum albumin (TDI-HSA) in vitro. RAGE knockdown cells were constructed and evaluated, and MK2006 (AKT inhibitor) was used in in vitro experiments.ResultsIn the TDI-induced asthmatic mice, airway reactivity, the level of Th2 cytokines in lymph supernatant, IgE, airway inflammation, and goblet cell metaplasia were all significantly increased. The increases were suppressed by FPS-ZM1, JNJ-26482585, and romidepsin. The expression of HDAC1, RAGE, and p-AKT/t-AKT was also upregulated in TDI-induced asthmatic mice, and the expressions were attenuated by FPS-ZM1. Knockdown of RAGE attenuated the upregulation of HDAC1 and phospho-AKT (p-AKT) in 16HBE cells stimulated by TDI-HSA. Treatment with the AKT inhibitor MK2006 suppressed TDI-induced HDAC1 expression. ConclusionRAGE mediates airway inflammation in a TDI-induced murine asthma model, partly via the HDAC1 pathway. Key words: Toluene diisocyanate, asthma, histone deacetylase 1, advanced glycosylation end product receptor
Multiple Anatomical Brain Networks for Self-esteem Related Analysis Among Young Adults
Authors: Peng Bo , Wang Suhong , Wee Chong-Yaw , Dai Yakang 
Published: August 2020

Abstract
Background: Self-esteem is the individual evaluation of oneself. People with high self-esteem have mental health and can bravely cope with the threats from the environment. With the development of neuroimaging techniques, researches on the cognitive neural mechanisms of self-esteem are increased. Existing methods based on brain morphometry and single-layer brain network cannot characterize the subtle structural differences related to self-esteem. Method: To solve this issue, we proposed multiple anatomical brain network framework based on multi-resolution ROI template to study the cognitive neuroscience mechanism of self-esteem. Multiple anatomical brain network consist of high-resolution ROI features extracted from structural MRI and hierarchal brain network features. For each layer, we calculated the correlation relationship between pairs of ROIs. In order to solve the high-dimensional problem caused by the large amount of multiple network features, combined feature selection methods are adopted to reduce the number of features while retaining discriminative information to the maximum extent. Multi-kernel SVM is employed to combine the two types of features by appropriate weight coefficient. Result: Our experimental results show that the proposed method can improve classification accuracy to 97.26% compared with single-layer brain network. Conclusions: This article reveals the cognitive neural mechanism of self-esteem and provides foundation for positive and healthy self-esteem.
Genetic association of G2/M checkpoint genes with susceptibility to HIV-1 infection and AIDS progression in northern Chinese MSM population
Authors: Wu Jiawei , Zhang Xuelong , Wang Kaili , Xu Lidan , Liu Bangquan , Sun Wenjing , Hu Yuanting , Jia Xueyuan , Sun Haiming , Wu Jie , Huang Yun , Ji Wei , Saleem Komal , Fu Songbin , Qiao Yuandong 
Published: August 2020

Abstract
Background

MSM has a high risk of HIV infection. Previous studies showed that the cell cycle regulation played an important role in HIV-1 infection, especially in G2/M checkpoint. The ATR, Chk1, Cdc25C and CDK1 are key genes in G2/M checkpoint. However, the association between the SNPs of these genes and susceptibility to HIV-1 infection and AIDS progression remains unknown.
Results

42 tSNPs of G2/M checkpoint genes were genotyped to analyze the association between these tSNPs and susceptibility to HIV-1 infection and AIDS progression among MSM (529 HIV − 1 seropositive men and 529 HIV-1 seronegative men) in northern China. The results showed that rs34660854-A and rs75368165-A in ATR gene and rs3756766-A in Cdc25C gene could increase the risk of HIV-1 infection (P = 0.049, P = 0.020 and P = 0.010, respectively), and ATR rs75069062 and Chk1 rs10893405 were significantly associated with AIDS progression (P = 0.026 and P = 0.029, respectively). Besides, rs34660854 and rs75368165 in ATR gene, rs12576279 and rs540436 in Chk1 gene, rs3756766 in Cdc25C gene and rs139245206 in CDK1 gene were significantly associated with HIV-1 infection under different models (P < 0.05).
Conclusions

The genetic variants of G2/M checkpoint genes had a molecular impact on the genesis of HIV-1 infection and AIDS progression in northern Chinese MSM population.
Use of Murine Models to Identify Tumor Immune Components that Correlate with Response to Immunotherapy in Breast Cancer
Authors: Castrillon Jessica A. , Mehta Anita K. , Cheney Emily , Miller Eric , Sotayo Alaba , Mittendorf Elizabeth A. , Letai Anthony , Guerriero Jennifer L. 
Published: August 2020

Abstract
Background: The heterogeneity of the breast tumor microenvironment (TME) may contribute to the lack of durable responses to immune checkpoint blockade (ICB), however, mouse models to test this are currently lacking. Proper choice and use of pre-clinical models are necessary for rigorous, pre-clinical studies to rapidly move laboratory findings into the clinic to treat patients. Methods: To elucidate how tumor latency and the heterogeneity of the TME contributes to ICB resistance, we performed comprehensive characterization of the TME using quantitative flow-cytometry and RNA expression analysis (NanoString) utilizing three distinct breast cancer models, all derived from the same autochthonous model. Tumor cells were obtained from the commonly used MMTV-PyMT murine breast cancer model and 1E6, 1E5 or 1E4 cells were either immediately injected into the mammary fat pad of FVB/NJ wild type mice or frozen (i.e. the tumor inoculation derived from the MMTV-PyMT tumors were never cultured). We then correlated the immunophenotyping to the efficacy observed from ICB. Results: These studies reveal that the number of cells used to generate syngeneic tumors significantly influences tumor latency, the infiltrating leukocyte population and response to ICB. The 4 models had vastly different TMEs which correlated to responses to ICB. Compared to the autochthonous model, all three syngeneic models had significantly more tumor infiltrating lymphocytes (TILs; CD3 + , CD4 + , and CD8 + ) and higher proportions of PD-L1 positive myeloid cells, whereas the MMTV-PyMT model had the highest frequency of myeloid cells out of total leukocytes. Increased TILs correlated with response to anti-PD-L1 and anti-CTLA-4 therapy; but tumor cell PD-L1expression and T-cell PD-1 expression did not. Conclusions: Here we have identified ICB-sensitive and resistant breast cancer models, generated from the same tumor cell inoculum. These models represent an opportunity to further interrogate the TME for breast cancer treatment and provide novel insights into therapeutic combinations and response to ICB. We believe this work serves as an important resource for the field to inform proper mouse model selection for pre-clinical studies.
Knowledge level of cardio-oncology in oncologist and cardiologist: A survey study in China
Authors: Liu Binliang , Wang Yanfeng , An Tao , Cheng Leilei , Liu Ying , Ou Jianghua , Li Hong , Zhao Xuemei , Xia Yunlong , Zhang Yuhui , Ma Fei 
Published: August 2020

Abstract
Background: With the rapid development of the field of cardio-oncology, it has been recognized as an important medical area to improve the healthcare quality of cancer patients with cardiac complications. In this study, we investigated the knowledge level of cardio-oncology of oncologists and cardiologists in China.Methods: A structured questionnaire survey was conducted during 30th September, 2018 to 3rd December, 2019. Questionnaires were given to oncologists and cardiologists randomly selected. A general linear model was used to explore the significant factors associated with the knowledge level.Results: In total, 973 responses were finally solicited and included in the analysis. Only 3 respondents reached the full marks. Results of knowledge level of cardio-oncology in China were not found satisfactory. The knowledge level was better in respondents with a cardiology background (P-value =0.001), worked in the higher-level hospital (P-value =0.01), from the east or west region of mainland China (P-value <0.001), at a high position of doctor (Chief doctor, P-value =0.009), with full awareness of cardio-oncology (P-value <0.001) and favorable attitude toward cardio-oncology development (P-value <0.001).Conclusions: Our survey suggested that the demands of cardio-oncology service in China was huge but the low level of knowledge on cardio-oncology was alarming. Strong supports are needed to improve the dedicated education of cardio-oncology in China
Relationship Between Electrode Position and Clinical Efficacy of Subthalamic Nucleus Deep Brain Stimulation and Motor Symptoms of Parkinson's Disease
Authors: Zhang Feng , Wang Feng , Li Weiguo , Wang Ning , Han Chunlei , Fan Shiying , Li Peng , Xu Lifeng , Zhang Jianguo , Meng Fangang 
Published: August 2020

Abstract
Objectives To investigate the relationship between the position of bilateral STN-DBS location of active contacts and clinical efficacy of STN-DBS in the motor symptoms of Parkinson’ disease (PD) patients. Methods We retrospectively analyzed the clinical data of 57 patients with PD who underwent bilateral STN-DBS from March 2018 to December 2018. UPDRS III scores, LEDD, PDQ-39 scores before operation and within 6 months after operation were determined. The location of activate contacts and volume of tissue activated (VTA) in the Montreal Neurological Institute (MNI) space, and their correlation with the rate of improvement of motor symptoms（UPDRS-Ⅲ score improvement rate）were examined. Results After 6 months of follow up, the UPDRS-Ⅲ scores of 57 patients（Med-off） were improved by 55.4±18.9% (P＜0.001) compared with that before operation. The improvement rate of PDQ-39 score [(47.4±23.2)%, (P<0.001)] and the reduction rate of LEDD [(40.1±24.3)% , (P<0.01)] at 6 months post surgery were positively correlated with the improvement rate of motor symptoms（Med-off）(PDQ-39:r=0.461, P＜0.001; LEDD:r=0.354, P=0.007)，the rate of improvement of UPDRS Ⅲ (Med-off) and the Z-axis coordinate of the active contact in the MNI space were positively correlated (left side:r=0.349,P=0.008;right eide:r=0.369,P=0.005). In the MNI space,there was no correlation between the UPDRS-Ⅲ score improvement rate (Med-off) at 6 months after operation and bilateral VTA in the STN motor subregion,STN associative subregion and STN limbic subregion of the active electrode contacts of 57 patients(all P>0.05).At 6 months after surgery, the difference between the Z-axis coordinate in the different improvement rate subgroups（＜25%, 25% to 50%, and＞50%） in the MNI space was statistically significant (left side: P=0.030; right side: P=0.024). In the MNI space, there was no statistically significant difference between the 3 groups in the VTA of the active electrode contacts (all P>0.05). Conclusions STN-DBS could improve the motor symptoms of PD patients and improved the quality of life. The closer the active stimulation contacts is to the dorsolateral sensorimotor area of STN, the better effect the DBS has on the motor symptoms of PD patients.
Hand hygiene and facemask use to prevent droplet transmitted viral diseases during air travel: a systematic literature review
Authors: De Angelis Giulia , Lohmeyer Franziska M. , Grossi Adriano , Posteraro Brunella , Sanguinetti Maurizio 
Published: August 2020

Abstract
Background: Transmission of airborne viral diseases (e.g. influenza A H1N1) takes mainly place in confined spaces including public travel by aircrafts. Adoption of hygiene measures may help to prevent the disease spread in air travel. This review summarizes the evidence on hand hygiene and use of facemask as viral disease prevention measures in aircrafts.Methods: A literature search was performed in PubMed, Scopus, and Web of Science databases up to 10 June 2020, according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses criteria. A PICOS (participants, intervention, comparator, outcome, and study design) approach was used to define the review question.Results: We included four studies, published between 2007 and 2020, all targeting the influenza virus disease, in the qualitative synthesis. Three studies used mathematical models, and two of them single flight models. The fourth was a case-control designed study to tracing an influenza outbreak in two flights during the 2009 influenza A H1N1 pandemic. Unlike the others, this study provided substantial evidence about the risk of not wearing a facemask in the airplane cabin during a flight from New York City to Hong Kong. Interestingly, none of 9 infected passengers compared to 15 (47%) of 32 healthy control passengers wore a mask (odds ratio, 0.0; 95% confidence interval, 0–0.7). In contrast, both case and control passengers appeared to be equally compliant in hand hygiene. Finally, we discussed the practicability of hygiene measures to control and prevent the SARS-CoV-2 transmission in confined air travel-related spaces.Conclusions: Facemask use combined with other hygiene measures may minimize the chance of droplet-transmitted virus (including SARS-CoV-2) spread by air travelers. However, more evidence is necessary before hygiene measures become an integral part of standard procedures in aircrafts.
Dys-regulation of Lnc-SNHG1 and miR-216b-5p Correlate With Chemo-resistance and Indicate Poor Prognosis of Serous Epithelial Ovarian Cancer
Authors: Pei Mei Li , Zhao Zong Xia , Shuang Ting 
Published: August 2020

Abstract
Background: Ovarian cancer is the most lethal of gynecological cancers and the 5-year survival rate is still low and chemo-resistance is the main obstacle for the treatment of ovarian cancer. Methods and Results: In this study, the human ovarian cancer tissue microarray was applied, 63 cases of chemo-sensitive serous EOC tissues and 32 cases of chemo-resistant serous EOC tissues were selected. By applying RNA fluorescence in situ hybridization (FISH), we found expression of Lnc-SNHG1 was up-regulated while miR-216b-5p showed low expression in chemo-resistant EOC patients compared with the chemo-sensitive group, both were mainly localized in the cytoplasm of the tissue cells. Chi-square test showed high lnc-SNHG1 level was significantly correlated with tumor stage, histological grade, nodal status, metastasis and chemo-resistance except tumor size. While there was no significant association between miR-216b-5p expression and parameters including tumor stage, histological grade, nodal status, metastasis, except chemo-resistance (P=0.0001). Spearman’s correlation analysis revealed significantly negative correlations between lnc-SNHG1 and miR-216b-5p (r = -0.424, P = 0.0001). Multivariate Cox regression analysis showed the expression of miR-216b-5p (P = 0.012, RR 2.137, 95 % CI 1.109–5.339) and FIGO stage (P = 0.001, RR 3.537, 95 % CI 1.72–7.276) was independent prognostic factors for the overall survival (OS) of serous EOC patients .While the FIGO stage (P = 0.003, RR2.237, 95 % CI 1.323–3.783) was the independent prognostic factor for the disease free survival (DFS) for the serous EOC patients. Kaplan- Meier curves revealed significant association of increased expression of lnc-SNHG1 with less OS and shorter DFS, while patients with low level of miR-216b-5p indicated less OS and DFS. Conclusions: In a word, we claimed over-expression of lnc-SNHG1 and decreased expression of miR-216b-5p were correlated with chemo-resistance of serous EOC patients and indicated less OS and shorter DFS of the patients.
Construction and identification of Rab7-ROSA26 knock-in mice by CRISPR/Cas9 technology
Authors: Xu Qing , Jin Linfang , Qi Xiaowei 
Published: August 2020

Abstract
Background: CRISPR/Cas9 system, a Genome editing technique, can achieve the purpose of gene knockout and gene insertion. Because we want to study the relationship between Rab7 and renal fibrosis in vivo, we should construct Rab7-knock-in mice. Results: We constructed Rab7-knock-in mice by CRISPR/Cas9 technology. Using the identification of PCR, southern blot and sequencing, the detected results were in line with our expectation. Conclusions: We constructed Rab7-knock-in mice successful, which can be provided for studying the relationship between Rab7 and renal fibrosis.
Blue-wavelength Light Therapy for Post-traumatic Brain Injury Sleepiness, Sleep Disturbance, Depression, Fatigue: A Systematic Review and Network Meta-analysis
Authors: Srisurapan Karan , Samakarn Yanisa , Kamklong Boonyasit , Siratrairat Phichayakan , Bumiputra Arina , Jaikwang Montita , Srisurapan Manit 
Published: August 2020

Abstract
Background: We aimed to determine the efficacy of blue-wavelength light therapy (BWLT) for post-traumatic brain injury (TBI) sleepiness, sleep disturbance, depression, and fatigue.Methods: We searched six databases for randomized controlled trials of BWLT for adults with a history of TBI. Outcomes of interest included sleepiness, sleep disturbance, depression, or fatigue. We conducted random-effect frequentist network meta-analyses (NMA) to compare the efficacy and a pairwise meta-analysis to examine the dropout rates.Results: We included four randomized controlled trials that compared BWLT, amber light therapy, and no light therapy. BWLT was significantly superior in reducing depression as compared to amber light therapy (SMD = 0.57, 95% CI = 0.04 to 1.10) and no light therapy (SMD = 0.81, 95% CI = 0.20 to 1.43). BWLT was also significantly superior in reducing fatigue as compared to amber light therapy (SMD = 1.00, 95% CI = 0.14 to 1.86) and no light therapy (SMD = 1.09, 95% CI = 0.41 to 1.76). There was no significant heterogeneity of depression and fatigue data (I2 = 0% for both). The relative risk of dropout rates (95% CI) shows no significant difference between BWLT and amber light therapy groups (RR = 3.72, 95% CI = 0.66 to 21.34, I2 = 0%).Conclusion: BWLT may be effective for post-TBI depression and fatigue. Due to the risks of bias and the inability to exclude publication bias among included studies, the current evidence remains insufficient to support the clinical application of BWLT for post-TBI depression and fatigue. Protocol registration: The protocol of this systematic review is available at Open Science Framework (https://osf.io/yf2qe/).
Effects of COVID-19 on Anxiety, Depression and Other Mental Health Issues: A worldwide scope review.
Authors: Machado Daiane Borges , Alves1 Flávia J.O. , Teixeira Camila S. S. , Rocha Aline S. , Castro-de-Araujo Luís FS , Singh Amandeep , Barreto Maurício L. 
Published: August 2020

Abstract
BACKGROUND: The COVID-19 pandemic has spread across the world and, along with it, a considerable degree of fear and uncertainties that impact on various aspects of societal life, including on people’s Mental Health (MH). Understanding how the COVID-19 pandemic affects MH can help to implement interventions and adequate public policies, providing more effective responses to mitigate its effects.OBJECTIVES: To summarize the scientific evidence on the possible influence of the Covid-19 pandemic on MH, critically evaluating the methods and scientific validity of the studies found, in addition to summarizing the recommendations on strategic measures to reduce the impact of COVID-19 on MH.METHODOLOGY: The current scoping review was conducted from a screening of 465 articles on COVID-19 and MH outcomes, based on the main database of scientific references on health, assessed throughout PubMed. RESULT: Of the 43 papers selected for summary and critical analysis 77% (n=33) indicated a relationship between the COVID-19 pandemic and anxiety symptoms, 56% (n=26) with depression or depressive symptoms, seven with changes in sleep pattern and seven with obsessive behaviours or Obsessive Compulsive Disorder. In addition to studies that showed an influence of the COVID-19 pandemic on trauma or post-traumatic stress disorder (PTSD), psychological distress, stress and fear. Most of the studies were carried out in China, and primarily evaluated the presence of anxiety and depression, through scales and questionnaires. Health professionals and the elderly were cited among the most affected population groups. CONCLUSIONS: Most studies presented significant methodological limits. Investments in new research, with controlled studies, including representative and randomized samples, and longitudinal follow-up, are necessary, to further explore the relationships between the COVID-19 pandemic and people's MH and long-term effects. Pending such studies, given the effects (during and after) of previous epidemics on MH, government measures need to be implemented to reduce the potential catastrophic effects of the COVID-19 pandemic on MH, and the burden that will remain after the pandemic. The timely identification of psychological distress and the identification of MH needs among populations, facilitate the development of targeted psychological interventions, in addition to the organization of health services and systems, during the pandemic.
Hepatoprotective Effect of Azolla microphylla on Isoproterenol-induced Rats and the Identification of Active Compound through HPTLC and GC-MS Analysis
Authors: Bhaskaran Sreenath K. , Kannappan Poornima , Muneeswari Perumalsamy , Madathil Sri R. 
Published: August 2020

Aim:
To study the pretreatment effect of ethanolic extract of Azolla microphylla (EAM)
on rat liver induced with Isoproterenol (ISO) and to identify the phytochemicals present in EAM using
HPTLC and GC-MS techniques.

Materials and Methods:
42 male Wistar rats were divided into 7 groups. Rats were pre-treated with
EAM (250 and 500 mg/kg bw) orally for 28 days and induced with ISO (85 mg/kg; intra-peritoneal)
on the 29th and 30th days. Blood and liver samples were collected from all the rats on the 30th day for
biochemical and histopathological observations. HPTLC and GC-MS analyses of EAM were done
using the standard protocols.

Results:
The ISO-induced group of rats displayed a significant decrease in the hepatic tissue level
and activities of total protein and Aspartate Transaminase (AST), Alanine Transaminase (ALT),
Alkaline Phosphatase (ALP) enzymes, respectively. Moreover, a significant decline in enzymatic and
non-enzymatic antioxidants levels was spotted in the same group. However, EAM pretreatment for
28 days significantly protected the rat liver from the aforementioned alterations. Nevertheless, histopathological
analysis revealed central vein dilation, necrosis, and infiltration of inflammatory cells in
the ISO-induced group, wherein, EAM pretreatment significantly protected the hepatocytes from the
above-mentioned changes indicating its antioxidant and cytoprotective potential. HPTLC analysis
displayed the presence of flavonoids. The GC-MS analysis confirmed the presence of quercetin in
EAM.

Conclusion:
The overall results suggest that EAM pretreatment possesses an ameliorative effect
against the ISO-induced oxidative damage in the rat hepatocytes.