bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating transcriptome and metabolome reveals molecular networks involved in genetic and environmental variation in tobacco
Authors: Liu Pingping , Luo Jie , Zheng Qingxia , Chen Qiansi , Zhai Niu , Xu Shengchun , Xu Yalong , Jin Lifeng , Xu Guoyun , Lu Xin , Xu Guowang , Wang Gangjun , Shao Jianfeng , Xu Hai-Ming , Cao Peijian , Zhou Huina , Wang Xusheng 
Published: April 2020

AbstractTobacco (Nicotiana tabacum) is one of the most widely cultivated commercial non-food crops with significant social and economic impacts. Here we profiled transcriptome and metabolome from 54 tobacco samples (2–3 replicates; n = 151 in total) collected from three varieties (i.e. genetic factor), three locations (i.e. environmental factor), and six developmental stages (i.e. developmental process). We identified 3,405 differentially expressed (DE) genes (DEGs) and 371 DE metabolites, respectively. We used quantitative real-time PCR to validate 20 DEGs, and confirmed 18/20 (90%) DEGs between three locations and 16/20 (80%) with the same trend across developmental stages. We then constructed nine co-expression gene modules and four co-expression metabolite modules , and defined seven de novo regulatory networks, including nicotine- and carotenoid-related regulatory networks. A novel two-way Pearson correlation approach was further proposed to integrate co-expression gene and metabolite modules to identify joint gene–metabolite relations. Finally, we further integrated DE and network results to prioritize genes by its functional importance and identified a top-ranked novel gene, LOC107773232, as a potential regulator involved in the carotenoid metabolism pathway. Thus, the results and systems-biology approaches provide a new avenue to understand the molecular mechanisms underlying complex genetic and environmental perturbations in tobacco.
Multimodality imaging derived energy loss index and outcome after transcatheter aortic valve replacement
Authors: Holy Erik W , Nguyen-Kim Thi Dan Linh , Hoffelner Lisa , Stocker Daniel , Stadler Thomas , Stähli Barbara E , Kebernik Julia , Maisano Francesco , Kasel Markus A , Frauenfelder Thomas , Ruschitzka Frank , Nietlispach Fabian , Tanner Felix C 
Published: April 2020

Abstract

Aims
To assess whether the combination of transthoracic echocardiography (TTE) and multidetector computed tomography (MDCT) data affects the grading of aortic stenosis (AS) severity under consideration of the energy loss index (ELI) in patients undergoing transcatheter aortic valve replacement (TAVR).

Methods and results
Multimodality imaging was performed in 197 patients with symptomatic severe AS undergoing TAVR at the University Hospital Zurich, Switzerland. Fusion aortic valve area index (fusion AVAi) assessed by integrating MDCT derived planimetric left ventricular outflow tract area into the continuity equation was significantly larger as compared to conventional AVAi (0.41 ± 0.1 vs. 0.51 ± 0.1 cm2/m2; P &lt; 0.01). A total of 62 patients (31.4%) were reclassified from severe to moderate AS with fusion AVAi being &gt;0.6 cm2/m2. ELI was obtained for conventional AVAi and fusion AVAi based on sinotubular junction area determined by TTE (ELILTL 0.47 ± 0.1 cm2/m2; fusion ELILTL 0.60 ± 0.1 cm2/m2) and MDCT (ELIMDCT 0.48 ± 0.1 cm2/m2; fusion ELIMDCT 0.61 ± 0.05 cm2/m2). When ELI was calculated with fusion AVAi the effective orifice area was &gt;0.6 cm2/m2 in 85 patients (43.1%). Survival rate 3 years after TAVR was higher in patients reclassified to moderate AS according to multimodality imaging derived ELI (78.8% vs. 67%; P = 0.01).

Conclusion
Multimodality imaging derived ELI reclassifies AS severity in 43% undergoing TAVR and predicts mid-term outcome.
Totally thoracoscopic ablation: a unilateral right-sided approach
Authors: Fleerakkers Jelle , Hofman Frederik N , van Putte Bart P 
Published: April 2020

Abstract
The aim of this article is to describe a unilateral approach for totally thoracoscopic ablation and left atrial appendage closure for the treatment of atrial fibrillation to simplify the procedure, avoid a technically more demanding thoracoscopy on the left side and potentially reduce postoperative pain without compromising the lesion set.
Overweight and obesity in shift workers: associated dietary and lifestyle factors
Authors: O’Brien Victoria M , Nea Fiona M , Pourshahidi L Kirsty , Livingstone M Barbara E , Bardon Laura , Kelly Ciara , Kearney John M , Corish Clare A 
Published: April 2020

Abstract

Background
Approximately 17% of the European Union workforce is engaged in shift work. Shift work has been associated with a number of chronic conditions, including obesity and obesity-related metabolic diseases. The aim of this study was to explore the dietary and lifestyle behaviours of shift workers with a healthy vs. overweight/obese body mass index (BMI).

Methods
A cross-sectional study was conducted on 1080 shift workers using a 15-min, telephone-administered questionnaire developed from qualitative research on Irish shift workers and national dietary intake data. Demographic and work-related factors, as well as dietary and lifestyle behaviours were recorded. BMI was calculated using self-reported height and weight. Univariate and multivariate logistic regression methods were used to analyze data according to BMI category.

Results
Over 40% of shift workers were classified as overweight or obese. Multivariate analysis indicated that being male [P &lt; 0.001, aOR = 2.102, 95% CI (1.62–2.73)] and middle- or older-aged were independently associated with overweight and obesity [P &lt; 0.001, aOR = 2.44 95% CI (1.84–3.24) and P &lt; 0.001, aOR = 2.9 95% CI (1.94–4.35), respectively]. Having a medium–high consumption of fried foods was independently associated with overweight and obesity [aOR = 1.38, 95% CI (1.06–1.8)].

Conclusions
Similar to the general population, overweight and obesity were strongly associated with male sex and middle- or older-age. Male shift workers may benefit from targeted dietary and lifestyle advice specifically focused on limiting fried foods to help protect against overweight and obesity.
Rebuilding depleted fisheries towards BMSY under uncertainty: harvest control rules outperform combined management measures
Authors: Sun Ming , Li Yunzhou , Ren Yiping , Chen Yong 
Published: April 2020

AbstractRebuilding depleted fisheries towards sustainable levels, such as BMSY, is challenging under uncertainty. Although a substantial amount of research has highlighted the importance of accounting for uncertainty in fisheries management, tactical measures remain to be identified. We consider two approaches to achieve this goal: (i) the naive maximum sustainable yield (MSY) approach, combining management measures based on effort control, catch quotas, and spatial–temporal closures, and (ii) the harvest control rules (HCRs) approach, developing HCRs based on short-term or long-term targets. A suite of strategies is developed accordingly and tested with management strategy evaluation for their performance under four sources of uncertainty that may negatively impact management effects, including reduced recruitment strength, increased natural mortality, inadequate implementation error, and varying levels of temporal effort aggregation. Combining management measures using the naive MSY approach is found to perform poorly in tackling uncertainty. Complex HCRs that account for both short-term and long-term BMSY targets can mitigate the adverse effects of uncertainty. The rebuilding target can be only achieved by compromising yield, especially when uncertainties with natural mortality and recruitment are present. Strategies based on catch quotas are prone to all sources of uncertainty, indicating latent risks in many current management practices.
Diagnosis of a primary pericardial mesothelioma by the combination of conventional echocardiography and contrast echocardiography
Authors: Wang Hongxia , Mou Yun , Zheng Zhelan 
Published: April 2020

Abstract
Primary malignancies arising from the pericardium are rare, even more unusual for primary pericardial mesothelioma. The diagnosis is difficult and has no standard treatment. We herein present a case of a 65-year-old woman with primary pericardial mesothelioma associated with dyspnoea and palpitations. Transthoracic conventional echocardiography revealed mild pericardial effusion and a hypo-echogenic mass in the pericardium. Contrast echocardiography showed that the mass was hyper-enhanced with a radial enhancement pattern. The patient underwent open chest exploration and was diagnosed by pathological examination. She had no further treatment and died 2 years later. In conclusion, the combination of conventional echocardiography and contrast echocardiography plays a significant role in diagnosing primary pericardial mesothelioma. Comprehensive evaluation and accurately preoperative diagnosis are important to exclude certain tumours that do not require surgery.
Cost of Hospitalization Associated With Respiratory Syncytial Virus Infection Versus Influenza Infection in Hospitalized Older Adults
Authors: Ackerson Bradley , An Jaejin , Sy Lina S , Solano Zendi , Slezak Jeff , Tseng Hung-Fu 
Published: April 2020

Abstract
Despite the severity of respiratory syncytial virus (RSV) disease in older adults, data on its costs are limited. We compared hospitalization costs for 2090 adults aged ≥ 60 years hospitalized with RSV or influenza by assigning direct health care costs. Hospitalization with RSV was associated with longer hospitalization and increased frequency of diagnosis-related groups for pulmonary complications, resulting in costs at least as great as those for influenza ($16 034 vs $15 163; 95% confidence interval for the difference, −$811 to $2547). Awareness of RSV disease burden in adults is needed to facilitate vaccination and treatment when they become available.
Indoor Environmental Factors and Acute Respiratory Illness in a Prospective Cohort of Community-Dwelling Older Adults
Authors: Han Lefei , Ran Jinjun , Chan Kwok-Hung , Mak Yim-Wah , Suen Lorna , Cowling Benjamin John , Yang Lin 
Published: April 2020

Abstract

Background
Ambient environmental factors have been associated with respiratory infections in ecological studies, but few studies have explored the impact of indoor environmental factors in detail. The current study aimed to investigate the impact of indoor environment on the risk of acute respiratory illness (ARI) in a subtropical city.

Method
A prospective cohort study was conducted in 285 community-dwelling older adults from December 2016 through May 2019. Individual household indoor environment data and ARI incidence were continuously collected. A time-stratified case-crossover analysis was conducted to estimate the excess risk of ARI associated with per-unit increase of daily mean indoor temperature, relative humidity, and absolute humidity (AH).

Result
In total, 168 episodes of ARI were reported with an average risk of 36.8% per year. We observed a negative association of ARI with indoor AH up to 5 lag days in cool seasons, with a 6-day cumulative excess risk estimate of −9.0% (95% confidence interval, −15.9% to −1.5%). Negative associations between household temperature or relative humidity and ARI were less consistent across warm and cool seasons.

Conclusions
Lower indoor AH in household was associated with a higher risk of ARI in the community-dwelling older adults in Hong Kong during cold seasons.
What patients “see” doctors in online fever clinics during COVID-19 in Wuhan?
Authors: Li Gang , Fan Guorui , Chen Yanyan , Deng Zhaohua 
Published: April 2020

Abstract

Objective
In December 2019, coronavirus disease 2019 (COVID-19) occurred in Wuhan, China. Online fever clinics were developed by hospitals, largely relieving the hospital’s burden. Online fever clinics could help people stay out of crowded hospitals and prevent the risk of cross infections. The objective of our study was to describe the patient characteristics of an online fever clinic and explore the most important concerns and question of online patients.

Materials and Methods
Our study extracted data from fever clinic records in medical information systems from January 24 to February 18, 2020 in a tertiary hospital in Wuhan. We described the characteristics of patients in fever clinic, then we extracted and classified questions of patient consultations through the online fever clinic dataset.

Results
For the 64 487 patients who attended the online fever clinic, the average age was 30.4 years, and 37 665 (58.4%) were female patients. The current state of patients from online were home without isolation (52 360 [81.2%]), home isolated (11 152 [17.29%]), and outpatient observation (975 [1.51%]). From the 594 patient questions analyzed, confirming diagnosis and seeking medical treatment account for 60.61% and 38.05%, respectively, followed by treating (25.59%), preventing (4.38%), and relieving anxiety (1.68%).

Discussion
Online fever clinics can effectively relieve patients’ mood of panic, and doctors can guide patients with suspected of COVID-19 to isolate and protect themselves through online fever clinic. Online fever clinics can also help to reduce the pressure of hospital fever clinics and prevent cross infection.

Conclusions
This study indicated the importance of online fever clinics during the COVID-19 outbreak for prevention and control.
Simultaneous Spectrophotometric Determination of Brilliant Blue and Tartrazine in Diverse Sample Matrices after Solid Phase Extraction
Authors: Bişgin Abdullah Taner 
Published: April 2020

Abstract

Background
Brilliant blue (BB) and tartrazine (TZ) are manufactured from petroleum and its products. These are the most popular consumed food dyes and are widely used in foodstuffs. Therefore, overuse of these dyes in foodstuffs and consumption of excessive amounts of these dyes can lead to health problems in humans.

Objective
The aim of this study was to develop a simple separation and preconcentration method for simultaneous spectrophotometric determination of BB and TZ dyes.

Methods
A column solid-phase separation extraction method combined with UV-Vis spectrophotometry was preferred and developed for single and simultaneous determination of BB and TZ dyes.

Results
The preconcentration factor was obtained as 80. Relative standard deviations were below than 4%. Detection limits of the method were determined as 0.29 and 1.21 µg/L for BB and TZ, respectively. Recovery values were obtained between 95–99% and 96–100% for BB and TZ, respectively. 10.9–235.7 µg/g and 1.7–8.0 µg/mL of BB contents of real samples were determined for solid and liquid samples, respectively. TZ concentrations of solid and liquid samples were ranged between 18.7–220.7 µg/g and 5.9–7.5 µg/mL, respectively.

Conclusions
Quantitative extraction results and satisfactory recovery values showed that method was successful and applicable for determination of BB and TZ concentrations in real pharmaceutical, industrial, and foodstuff samples.

Highlights
The method has exhibited a high preconcentration factor and effective separation against to matrix ions. The method did not need an experienced operator with high operation experience. Elution solvent can be chosen according to the availability of the chemicals in the laboratory and cheapness of the chemicals.
Report on the Emergence Time of a Species of Thitarodes Ghost Moth (Lepidoptera: Hepialidae), Host of the Caterpillar Fungus Ophiocordyceps sinensis (Ascomycota: Ophiocordycipitaceae) in Uttarakhand, India
Authors: Wang Zhengyang , Yang Darong , Janak Rawal , Pierce Naomi E 
Published: April 2020

Abstract
Ghost moths in the genus Thitarodes Viette, 1968, are hosts of the economically important caterpillar fungus, which is harvested in high mountain meadows across the Hengduan mountains and the Himalayas. In northwestern India, although caterpillar fungus has been collected in the state of Uttarakhand, no adults of Thitarodes species have been recorded. We report the sighting of a female pupa of Thitarodes sp. and its last-instar molt at Munsiyari, Uttarakhand, India, on 5 July 2019 and estimate that the adult emergence time in this habitat should be late July or early August. Although the habitats of caterpillar fungus in Uttarakhand are geographically closer to those in Nepal, they are climatically more similar to habitats of caterpillar fungus in Qinghai province in China. Among records at the same elevation, climatic variables are highly predictive of the emergence date of Thitarodes adults (adjusted-R2: 0.7925, F = 6.27, P = 0.03). Our result highlights the role of the Himalayan mountains as both a north–south climatic barrier and an east–west climatic gradient. We encourage local stakeholders and scientists in Uttarakhand to survey adult emergences of Thitarodes from July to mid-August.
Elevated Cerebrospinal Fluid and Plasma N-Cadherin in Alzheimer Disease
Authors: Choi Ji-Young , Cho Sun-Jung , Park Jung Hyun , Yun Sang-Moon , Jo Chulman , Kim Eun-Joo , Huh Gi Yeong , Park Moon Ho , Han Changsu , Koh Young Ho 
Published: April 2020

Abstract
N-cadherin is a synaptic adhesion molecule stabilizing synaptic cell structure and function. Cleavage of N-cadherin by γ-secretase produces a C-terminal fragment, which is increased in the brains of Alzheimer disease (AD) patients. Here, we investigated the relationship between fluid N-cadherin levels and AD pathology. We first showed that the cleaved levels of N-cadherin were increased in homogenates of postmortem brain from AD patients compared with that in non-AD patients. We found that cleaved N-cadherin levels in the cerebrospinal fluid were increased in AD dementia compared with that in healthy control. ELISA results revealed that plasma levels of N-cadherin in 76 patients with AD were higher than those in 133 healthy control subjects. The N-cadherin levels in the brains of an AD mouse model, APP Swedish/PS1delE9 Tg (APP Tg) were reduced compared with that in control. The N-terminal fragment of N-cadherin produced by cleavage at a plasma membrane was detected extravascularly, accumulated in senile plaques in the cortex of an APP Tg mouse. In addition, N-cadherin plasma levels were increased in APP Tg mice. Collectively, our study suggests that alteration of N-cadherin levels might be associated with AD pathology.
A hybrid treatment modality of a subtrochanteric femoral fracture in a patient with osteoporosis due to a renal Fanconi syndrome: a case report
Authors: Lange Stefan F , Schrooten Tim K J , de Wit Ralph J , de Groot Reinier 
Published: April 2020

Abstract
A 24-year-old male with an idiopathic renal Fanconi syndrome presented to our ER after a low-energetic fall. Conventional imaging revealed a right subtrochanteric femoral fracture, severely decreased bone quality and cannulated collum femoris screws on the contralateral side. Regular plate-screw osteosynthesis or cephalomedullary implantation was deemed insufficient, due to a high iatrogenic and periprosthetic fracture probability. The decision was made to perform a plate-screw osteosynthesis combined with an intramedullary polymer bone enhancement (IlluminOss), to minimize this risk. No complications occurred perioperatively. The patient was able to walk independently two months postoperatively. This case shows that use of polymer implant as an enhancement of osteosynthesis in repair of fractures in the Fanconi syndrome is a safe and possible useful treatment method.
Preventing spread of SARS-CoV-2 and preparing for the COVID-19 outbreak in the surgical department: perspectives from two Scandinavian countries
Authors: Lindeman Robbert-Jan , Sund Malin , Löfgren Jenny , Basso Trude , Søreide Kjetil 
Published: April 2020

ABSTRACT
A COVID-19 pandemic was declared on March 11 by the World Health Organization (WHO). The first cases of COVID-19 were confirmed on January 31 in Sweden and on February 26 in Norway. Despite being similar countries with universal healthcare systems, the governmental approaches to mitigation of the epidemic have varied considerably. Norway initiated a societal lockdown effective from March 12, the same day as the first confirmed death. Sweden has initiated a more laxed and gradual strategy based on the appeal for a strong personal sense of responsibility to mitigate the viral spread. In both countries, the first weeks of preparation has seen a strong reduction in elective surgery, with several implemented principles to mitigate SARS-CoV-2 spread and prepare for surgical care for COVID-19 diseases as needed. This invited leading article gives a brief overview of some of the early experiences of the outbreak in two Scandinavian countries.
Solving a Paradox of Evidential Equivalence
Authors: Dorr Cian , Hawthorne John , Isaacs Yoaav 
Published: April 2020

Abstract
David Builes presents a paradox concerning how confident you should be that any given member of an infinite collection of fair coins landed heads, conditional on the information that they were all flipped and only finitely many of them landed heads. We argue that if you should have any conditional credence at all, it should be 1/2.
Deficiency of the onco-miRNA cluster, miR-106b∼25, causes oligozoospermia and the cooperative action of miR-106b∼25 and miR-17∼92 is required to maintain male fertility
Authors: Hurtado Alicia , Palomino Rogelio , Georg Ina , Lao Miguel , Real Francisca M , Carmona F David , Burgos Miguel , Jiménez Rafael , Barrionuevo Francisco J 
Published: April 2020

Abstract
The identification of new genes involved in sexual development and gonadal function as potential candidates causing male infertility is important for both diagnostic and therapeutic purposes. Deficiency of the onco-miRNA cluster miR-17∼92 has been shown to disrupt spermatogenesis, whereas mutations in its paralog cluster, miR-106b∼25, that is expressed in the same cells, were reported to have no effect on testis development and function. The aim of this work is to determine the role of these two miRNA clusters in spermatogenesis and male fertility. For this, we analyzed miR-106b∼25 and miR-17∼92 single and double mouse mutants and compared them to control mice. We found that miR-106b∼25 knock out testes show reduced size, oligozoospermia and altered spermatogenesis. Transcriptomic analysis showed that multiple molecular pathways are deregulated in these mutant testes. Nevertheless, mutant males conserved normal fertility even when early spermatogenesis and other functions were disrupted. In contrast, miR-17∼92+/−; miR-106b∼25−/− double mutants showed severely disrupted testicular histology and significantly reduced fertility. Our results indicate that miR-106b∼25 and miR-17∼92 ensure accurate gene expression levels in the adult testis, keeping them within the required thresholds. They play a crucial role in testis homeostasis and are required to maintain male fertility. Hence, we have identified new candidate genetic factors to be screened in the molecular diagnosis of human males with reproductive disorders. Finally, considering the well-known oncogenic nature of these two clusters and the fact that patients with reduced fertility are more prone to testicular cancer, our results might also help to elucidate the molecular mechanisms linking both pathologies.