bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system
Authors: Ozel-Tasci Cansu , Pilatin Gozde , Edeer Ozgur , Gulec Sukru 
Published: August 2020

Abstract

Background
Functional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.

Objective
We aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.

Materials and methods
Cake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.

Results and discussion
The glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.

Conclusion
Our results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.
HPLC and LC-MS/MS measurement methods for the quantification of asymmetric dimethylarginine (ADMA) and related metabolites
Authors: Unlu Ali , Eryavuz Onmaz Duygu , Abusoglu Sedat , Abusoglu Gulsum 
Published: August 2020

Abstract
Methyl arginine derivatives such as asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA), L-N-monomethyl arginine (L-NMMA) are formed by proteolytic catalysis following methylation of arginine residues in proteins. These metabolites reduce NO production. Methylated arginines are an important biomarker for various diseases such as cardiovascular and renal diseases. Therefore, many methods have been developed to reliably and accurately measure the levels of these metabolites. This review, HPLC and LC-MS/MS methods developed for the measurement of methylarginine derivatives are discussed. In HPLC methods, solid phase extraction, derivatization and subsequent separation by reverse phase chromatography were performed. Since these metabolites are polar, they are difficult to retain in conventional reverse phase columns. In addition, as serum levels of these metabolites are low, sensitivity problems have been observed in HPLC methods. Derivatization has been applied to eliminate these problems. However, there have been problems with the stability of derivatives formed. Another important problem is that the separation of stereoisomer ADMA and SDMA can only be achieved chromatographically. Tandem mass spectrometric methods are accurate, selective, sensitive and rapid since analytes are separated depending on m/z ratios rather than chromatographic separation. Therefore, tandem mass spectrometry methods might be considered as the goal standard for these analytes.
Structural and wavelength dependent optical study of thermally evaporated Cu2Se thin films
Authors: Yadav Brijesh Kumar , Singh Pratima , Yadav Chandreshvar Prasad , Pandey Dharmendra Kumar , Singh Dhananjay 
Published: August 2020

Abstract
The present work encloses structural and optical characterization of copper (I) selenide (Cu2Se) thin films. The films having thickness 85 nm have been deposited using thermal evaporation technique in initial step of work. The structural and morphological studies of deposited thin films are then done by X-ray diffraction (XRD), scanning electron microscope (SEM), and surface profilometer measurements. Later on, ultraviolet-visible-near-infrared (UV-VIS-NIR) spectrophotometer and Raman spectroscopic measurements are performed for optical characterization of films. The structure and morphology measurements reveal that deposited material of films is crystalline. The optical band gap estimated from the optical transmission spectra of the film has been found 1.90 eV. The mean values of refractive index, extinction coefficient, real and imaginary dielectric constant are received 3.035, 0.594, 9.623, and 3.598, respectively. The obtained results are compared and analyzed for justification and application of Cu2Se thin films.
Der finale Entwurf einer Verordnung über Europäische Schwarmfinanzierungsdienstleister (ECSP-VO) – Anwendungsbereich und Regelungsüberblick
Authors: Rusch Konrad 
Published: August 2020

Zusammenfassung
Dieser Beitrag analysiert den am 8. 7. 2020 in deutscher amtlicher Übersetzung erschienenen, finalen Entwurf einer Verordnung über Europäische Schwarmfinanzierungsdienstleister und klärt zentrale Fragen ihres Anwendungsbereiches vor dem Hintergrund bereits etablierter Schwarmfinanzierungsmodelle.
Gradient-based Learning Methods Extended to Smooth Manifolds Applied to Automated Clustering
Authors: Koudounas Alkis , Fiori Simone 
Published: August 2020

Grassmann manifold based sparse spectral clustering is a classification technique that consists in learning a latent representation of data, formed by a subspace basis, which is sparse. In order to learn a latent representation, spectral clustering is formulated in terms of a loss minimization problem over a smooth manifold known as Grassmannian. Such minimization problem cannot be tackled by one of traditional gradient-based learning algorithms, which are only suitable to perform optimization in absence of constraints among parameters. It is, therefore, necessary to develop specific optimization/learning algorithms that are able to look for a local minimum of a loss function under smooth constraints in an efficient way. Such need calls for manifold optimization methods. In this paper, we extend classical gradient-based learning algorithms on at parameter spaces (from classical gradient descent to adaptive momentum) to curved spaces (smooth manifolds) by means of tools from manifold calculus. We compare clustering performances of these methods and known methods from the scientific literature. The obtained results confirm that the proposed learning algorithms prove lighter in computational complexity than existing ones without detriment in clustering efficacy.
The impact of factor infusion frequency on health-related quality of life in people with haemophilia
Authors: Pedra Gabriel , Christoffersen Pia , Khair Kate , Lee Xin Ying , O’Hara Sonia , O’Hara Jamie , Pasi John 
Published: August 2020

Abstract

Background
Some studies suggest that people with haemophilia (PwH) who use prophylaxis value low frequency of clotting factor administration more than a lower risk of bleeding. However, more frequent infusions offer the potential of reducing joint disease and pain, which in turn may improve functioning and quality of life.

Aims
To explore the impact on health-related quality of life (HRQoL) aspects of haemophilia associated with adherence and annual infusion rate in the context of factors influencing treatment that are important to patients, including prophylaxis, chronic pain, concomitant conditions and hospital admission.

Materials and methods
HRQoL was assessed in participants with severe haemophilia in the ‘Cost of Haemophilia in Europe: a Socioeconomic Survey’ (CHESS) study who were using prophylaxis. Patients using on-demand treatment were excluded. This multivariate analysis examined the interaction between factors potentially influencing treatment and HRQoL, and minor and major bleeds.

Results
From the total CHESS population (n=1,285), 338 (26%) participants provided responses for major and minor bleeds and target joints, and 145 (11%) provided EQ-5D-3L responses. Major and minor bleeds were associated with pain. Patients with severe chronic pain reported a substantial negative impact on HRQoL; but this was significantly improved by increases in the annual infusion rate. This was not apparent in participants with mild or moderate pain.

Conclusion
Increasing the frequency of prophylaxis infusions is associated with improved quality of life in PwH who have severe chronic pain. However, increasing the number of infusions per week in those with mild or moderate chronic pain with the intention of improving prophylactic effect may not have the same effect.
PERMAINAN TRADISIONAL KAULINAN BARUDAK UNTUK MENGEMBANGKAN KEMAMPUAN MENGELOLA EMOSI DIRI SENDIRI ANAK USIA DINI
Authors: Mulyadiprana Ahmad , Ganda Nana , WS Rustono 
Published: August 2020

This research is based on the facts in the field regarding the process of achieving early childhood education that is not optimal. Eeducational attainment is about to manage sel emotion of early childhood. The traditional games "kaulinan barudak" are one of the learning process approaches could be expected to provide positive stimulation toward developmental aspect of children’s potential. This study is conducted to determine the contribution of traditional games "kaulinan barudak" to the development of self emotion manage of children aged 5-6 years. This study uses quasi-experimental method with Pretest and Posttest control group design. Data is collected using structured observation, notes, and documentation. The results of this study are 1) the ability of self emotion manage of children aged 5-6 years in kindergarten Artanita Tasikmalaya entered into category”positive significant”; 2) the ability of self emotion manage of children aged 5-6 years of conventional game in kindergarten Artanita Tasikmalaya entered into category”no siginificant positive effect”; 3) the ability of self emotion manage of children aged 5-6 years in kindergarten Artanita Tasikmalaya entered into category”Different effect of traditional game “kaulinan barudak” from the conventional game”. The traditional game of “kaulinan barudak” gives a more significant positive effect than the conventional game. Penelitian ini dilatarbelakangi oleh fakta-fakta di lapangan mengenai kondisi proses pencapaian pendidikan anak usia dini yang belum optimal. Proses pencapaian pendidikan yang dimaksud adalah kemampuan mengelola emosi diri sendiri anak usia dini. Permainan tradisional “kaulinan barudak” merupakan salah satu pendekatan proses belajar yang diduga dapat memberikan stimulasi positif terhadap aspek perkembangan potensi anak. Penelitian ini dilakukan untuk mengetahui kontribusi permainan tradisional “kaulinan barudak” terhadap pengembangan kemampuan mengelola emosi diri sendiri anak usia 5-6 tahun. Penelitian ini menggunakan metode kuasi eksperimen dengan Pretest-Posttest control group design. Teknik pengumpulan data menggunakan observasi terstruktur, catatan lapangan dan dokumentasi. Hasil penelitian menunjukkan bahwa 1) Terdapat pengaruh yang positif dan signifikan penerapan permainan tradisional kaulinan barudak terhadap pengembangan kemampuan mengelola emosi diri sendiri anak usia 5-6 tahun di TK Artanita Kota Tasikmalaya; 2) Tidak terdapat pengaruh positif yang signifikan penerapan permainan konvensional terhadap pengembangan kemampuan mengelola emosi diri sendiri anak usia 5-6 tahun di TK Negeri Pembina Kota Tasikmalaya; 3) Terdapat perbedaan pengaruh permainan tradisional kaulinan barudak dengan permainan konvensional terhadap pengembangan kemampuan mengelola emosi diri sendiri anak usia 5-6 tahun. Permainan tradisional kaulinan barudak memberikan pengaruh positif yang lebih signifikan dari pada permainan konvensional.
KEMAMPUAN MEMBACA PERMULAAN MELALUI PENGGUNAAN MEDIA PAPAN FLANEL ANAK USIA 4-5 TAHUN
Authors: Hasanah Lathipah , Nurhasanah Aini 
Published: August 2020

ABSTRACTThe purpose of this research is improve the early reading ability in the child age 4-5 years through used flannel board. The subject of this research is children in grade A, TKI Nurul Hidayah, Bekasi Jaya Indah. The method used classroom action research. This research used qualitative and quantitative approach according to Kemmis model which consists of four components: planning, action, observation and reflection. The mean value obtained in pre intervention was 31.6%. Based on these results, intervention must be taken through used flannel board. After doing intervention in cycle I, The results obtained an average score of 51.1%. Then intervention in cycle II and obtained an average value of 75.4%. Based on these results, the early reading ability has increased according to determined criteria, so that intervention action is stopped. Based on the results of research that has been implemented, using flannel board very effective can improve the early reading ability. Penelitian ini bertujuan untuk meningkatkan kemampuan membaca permulaan pada anak usia 4-5 tahun melalui penggunaan media papan flanel. Subjek penelitian ini adalah anak-anak kelompok A di TKI Nurul Hidayah, Bekasi Jaya Indah. Metode penelitian yang dilakukan oleh peneliti adalah metode penelitian tindakan kelas. Pendekatan yang digunakan adalah pendekatan kualitatif dan kuantitatif menurut model Kemmis yang terdiri dari empat komponen, yaitu: perencanaan, tindakan, observasi dan refleksi. Nilai rata-rata yang didapat pada praintervensi adalah 31.6%. Berdasarkan hasil ini maka harus dilakukan tindakan intervensi melalui kegiatan bermain balok. Setelah melakukan intervensi tindakan siklus I maka diperoleh hasil nilai rata-rata sebesar 51.1%. Kemudian dilakukan intervensi tindakan siklus II dan diperoleh nilai rata-rata 75.4%. Berdasarkan hasil ini maka kemampuan membaca permulaan anak telah meningkat sesuai dengan kriteria yang telah ditentukan sehingga tindakan intervensi dihentikan. Berdasarkan hasil penelitian yang telah dilaksanakan, penggunaan media papan flanel sangat efektif dapat meningkatkan kemampuan membaca permulaan pada anak.
KEMAMPUAN BERHITUNG PERMULAAN ANAK TAMAN KANAK-KANAK KELOMPOK B SE-KELURAHAN LENGKONGSARI KOTA TASIKMALAYA
Authors: Maesaroh Maya , Sumardi Sumardi , Nur Lutfi 
Published: August 2020

ABSTRACTThis study aims to determine the ability to count the beginning of kindergarten children group B village Lengkongsari Tasikmalaya City. This is based on the importance of numeracy skills for early childhood. In addition, the ability to start counting also needs to be developed so that children have the readiness to continue to higher education again that is elementary school. This research is descriptive research using survey method. Population in this research is kindergarten children group B Se-village Lengkongsari Tasikmalaya City. The data retrieval technique is using purposive sampling. The population of this research is Children of Kindergarten of Lengkongsari Urban Village. The results of data analysis of early numeracy skills include the ability to split the numbers 1-10, write 1-10 numbers, the ability of addition and reduction of 1-10 numbers. The ability to spell shows an average of 9.87. In terms of criteria according to Acep Yoni the ability to count is very well developed. Ability to write an average of 9.53 and is in the criteria developed very well. Furthermore, the summation ability of 9.25 and the average abatement capability is 9.08 and is in excellent growing criteria. ABSTRAKPenelitian ini bertujuan untuk mengetahui kemampuan berhitung permulaan anak TK kelompok B Se-kelurahan Lengkongsari Kota Tasikmalaya. Hal ini dilatar belakangi oleh pentingnya kemampuan berhitung untuk anak usia dini. Selain itu kemampuan berhitung permulaan juga perlu dikembangkan sehingga anak memiliki kesiapan melanjutkan ke pendidikan yang tinggi lagi yaitu sekolah dasar. Penelitian ini adalah penelitian deskriptif dengan menggunakan metode survey. Populasi dalam penelitian ini adalah anak TK kelompok B Se-kelurahan Lengkongsari Kota Tasikmalaya. Teknik pengambilan data yaitu menggunakan sampling purposive. Hasil analisis data mengenai kemampuan berhitung permulaan yang meliputi kemampuan membilang angka 1-10, menulis angka 1-10, kemampuan penjumlahan dan pengurangan angka 1-10. Kemampuan membilang menunjukan rata-rata sebesar 9,87. Kemampuan membilang sudah berkembang sangat baik (BSB). Kemampuan menulis rata-rata sebesar 9,53 dan berada dalam kriteria berkembang sangat baik. Selanjutnya kemampuan penjumlahan 9,25 dan kemampuan pengurangan rata-rata sebesar 9,08 dan berada dalam kriteria berkembang sangat baik (BSB).
PENINGKATAN KEMAMPUAN MOTORIK HALUS ANAK USIA DINI MELALUI KOLASE BERBAHAN SERBUK KAYU PADA KELOMPOK B DI RA YASBIMAN AL-MUNAWAR KABUPATEN TASIKMALAYA
Authors: Nurmala Wida , Yasbiati Yasbiati , Rahman Taopik 
Published: August 2020

ABSTRACTSmooth motor skills are abilities that involve muscles in certain parts of body, such as fingers. One of the activities that can improve the fine motor skills of early childhood is through collage activities made from wood powder. This study aims to improve the fine motor skills of early childhood through collections made from wood powder in group B in RA Yasbiman Al-Munawar Tasikmalaya District. The fine motor aspects studied are the motion of both hands, the skill of moving the limbs associated with the fingers, and the skills are able to coordinate the eye by hand. This type of research is Classroom Action Research. The research model from Kemmis and Mc. Taggart. The Data analysis in this study are used qualitative data and quantitative data. Subjects in this study were early childhood group B RA Yasbiman Al-Munawar amounted to 15 people, namely 6 boys and 9 girls. The results of this study showed that the fine motor skills of children have increased after giving them the collage activity of wood powder. ABSTRAKKemampuan motorik halus adalah kemampuan yang melibatkan otot-otot pada bagian tubuh tertentu seperti jari tangan. Salah satu kegiatan yang dapat meningkatkan kemampuan motorik halus anak usia dini yaitu melalui kegiatan kolase berbahan serbuk kayu. Penelitian ini bertujuan untuk peningkatan kemampuan motorik halus anak usia dini melalui kolase berbahan serbuk kayu pada kelompok B di RA Yasbiman Al-Munawar Kabupaten Tasikmalaya. Aspek motorik halus yang diteliti antara lain, keterampilan gerak kedua tangan, keterampilan menggerakan anggota tubuh yang berhubungan dengan jari-jemari, dan keterampilan yang mampu mengkoordinasikan mata dengan tangan. Jenis penelitian ini yaitu Penelitian Tindakan Kelas. Dengan model penelitian Kemmis dan Mc. Taggart. Analisis data dalam penelitian ini peneliti menggunakan data kualitatif dan data kuantitatif. Subjek dalam penelitian ini adalah anak usia dini kelompok B RA Yasbiman Al-Munawar berjumlah 15 orang, yaitu 6 orang anak laki-laki dan 9 orang anak perempuan. Hasil penelitian ini menunjukan bahwa kemampuan motorik halus anak mengalami peningkatan setelah diberikan kegiatan kolase berbahan serbuk kayu.
PENGEMBANGAN MEDIA HEALTHY DENTAL BOX (HDB) UNTUK MEMFASILITASI KETERAMPILAN MENGGOSOK GIGI ANAK USIA 4-5 TAHUN
Authors: Najiah Irna , Nur Lutfi , Rahman Taopik 
Published: August 2020

This research is based on the results of preliminary studies that have been conducted by researchers regarding the importance of brushing teeth. Brushing teeth in children aged 4-5 years is not explained in detail in the curriculum and has an impact on the difficulties faced by teachers in selecting and designing learning media to brush their teeth, therefore researchers design learning media with approaches that are appropriate to the conditions of children in the field. Researchers developed the tooth puppet media into a Healthy Dental Box (HDB) medium to facilitate the brushing skills of children aged 4-5 years. The method used in this research is Design Based Research (DBR) developed by Reeves to design, develop, and test the feasibility of a product to overcome problems in learning. Data collection is done by observation, interview and validation of media experts. In general, the product is declared suitable for use as a learning medium regarding brushing teeth based on the results of media validation by experts in the health sector, experts in the field of learning media and pedagogical experts. Reflections on product development, namely producing Healthy Dental Box (HDB) media to facilitate tooth brushing skills in children aged 4-5 years. Penelitian ini didasarkan pada hasil studi pendahuluan yang telah dilakukan oleh peneliti mengenai pentingnya menggosok gigi. Menggosok gigi pada anak usia 4-5 tahun tidak dijelaskan secara rinci dalam kurikulum dan berdampak pada kesulitan yang dihadapi guru dalam memilih dan merancang media pembelajaran menggosok gigi anak, untuk itu peneliti merancang media pembelajaran dengan pendekatan yang sesuai dengan kondisi anak di lapangan. Peneliti mengembangkan media boneka gigi menjadi media Healthy Dental Box (HDB) untuk memfasilitasi keterampilan menggosok gigi anak usia 4-5 tahun. Metode yang digunakan dalam penelitian ini adalah Design Based Research (DBR) yang dikembangkan oleh Reeves untuk merancang, mengembangkan, dan menguji kelayakan suatu produk untuk mengatasi masalah dalam pembelajaran. Pengumpulan data dilakukan dengan observasi, wawancara dan validasi ahli media. Secara umum produk dinyatakan layak digunakan sebagai media pembelajaran mengenai menggosok gigi berdasarkan hasil validasi media oleh ahli dalam bidang kesehatan, ahli bidang media pembelajaran dan ahli pedagogik. Refleksi dari pengembangan produk, yaitu menghasilkan media Healthy Dental Box (HDB) untuk memfasilitasi keterampilan menggosok gigi pada anak usia 4-5 tahun.
PENGEMBANGAN DASAR KEBUTUHAN RENCANA KEGIATAN PEMBELAJARAN BERORIENTASI SAINS PADA SUB TEMA AIR UNTUK MENGOPTIMALKAN KETERAMPILAN MENGAMATI ANAK USIA DINI
Authors: Adawiyah Wasilah Nur , Mulyana Edi Hendri , Elan Elan 
Published: August 2020

Research and development has been carried out, which aims find out the basic needs of developing scienceoriented learning activity plans on the water sub theme to optimize early childhood observation skills. Metode penelitian yang dilakukan menggunakan metode pengembangan, model pendekatan mix method dengan teknik Educational Design Research mengacu pada model menurut McKenney dan Reeves. This article only explains the Educational Design Research engineering research in the first stage, namely the exploration and analysis of research problems. Data collection was carried out with preliminary studies, namely field studies with initial observations and literature studies. The field study was conducted at Raudhatul Athfal AT-Taufiq which is located in Cibeureum Sub-district, Tasikmalaya City. Literature study is carried out by examining the journals and the results of previous research related to problems and science-oriented books in early childhood. Thus, the results of the study indicate various basic needs for developing science-oriented learning activity plans to optimize observational skills, which will later be used as a reference or guideline for designing research development products in the form of learning activity plans. Telah dilakukan penelitian dan pengembangan, yang bertujuan untuk mengetahui dasar kebutuhan pengembangan rencana kegiatan pembelajaran berorientasi sains pada sub tema air untuk mengoptimalkan keterampilan mengamati anak usia dini. Metode penelitian yang dilakukan menggunakan metode pengembangan, model pendekatan mix method dengan teknik Educational Design Research mengacu pada model menurut McKenney dan Reeves. Artikel ini hanya menjelaskan penelitian teknik Educational Design Research pada tahap pertama yaitu tahap eksplorasi dan analisis masalah penelitian. Pengumpulan data dilakukan dengan studi pendahuluan yaitu studi lapangan dengan observasi awal dan studi literatur. Studi lapangan dilaksanakan di Raudhatul Athfal AT-Taufiq yang beralamat di Kecamatan Cibeureum Kota Tasikmalaya. Studi literatur dilakukan dengan mengkaji jurnal dan hasil penelitian terdahulu terkait permasalahan serta buku-buku berorientasi sains pada anak usia dini. Sehingga, hasil penelitian menunjukan berbagai kebutuhan dasar untuk mengembangkan rencana kegiatan pembelajaran berorientasi sains untuk mengoptimalkan keterampilan mengamati, yang nantinya akan dijadikan sebagai acuan atau pedoman untuk perancangan produk pengembangan penelitian berupa rencana kegiatan pembelajaran.