bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple QTL Mapping in Autopolyploids: A Random-Effect Model Approach with Application in a Hexaploid Sweetpotato Full-Sib Population
Authors: da Silva Pereira Guilherme , Gemenet Dorcus C , Mollinari Marcelo , Olukolu Bode A , Wood Joshua C , Diaz Federico , Mosquera Veronica , Gruneberg Wolfgang J , Khan Awais , Buell C Robin , Yencho G Craig , Zeng Zhao-Bang 
Published: May 2020

Abstract
Genetic analysis in autopolyploids is a very complicated subject due to the enormous number of genotypes at a locus that needs to be considered. For instance, the number of...
In developing countries, the sweetpotato, Ipomoea batatas (L.) Lam. (2n=6x=90), is an important autopolyploid species, both socially and economically. However, quantitative trait loci (QTL) mapping has remained limited due to its genetic complexity. Current fixed-effect models can fit only a single QTL and are generally hard to interpret. Here, we report the use of a random-effect model approach to map multiple QTL based on score statistics in a sweetpotato biparental population (‘Beauregard’ × ‘Tanzania’) with 315 full-sibs. Phenotypic data were collected for eight yield component traits in six environments in Peru, and jointly adjusted means were obtained using mixed-effect models. An integrated linkage map consisting of 30,684 markers distributed along 15 linkage groups (LGs) was used to obtain the genotype conditional probabilities of putative QTL at every centiMorgan position. Multiple interval mapping was performed using our R package QTLpoly and detected a total of 13 QTL, ranging from none to four QTL per trait, which explained up to 55% of the total variance. Some regions, such as those on LGs 3 and 15, were consistently detected among root number and yield traits, and provided a basis for candidate gene search. In addition, some QTL were found to affect commercial and noncommercial root traits distinctly. Further best linear unbiased predictions were decomposed into additive allele effects and were used to compute multiple QTL-based breeding values for selection. Together with quantitative genotyping and its appropriate usage in linkage analyses, this QTL mapping methodology will facilitate the use of genomic tools in sweetpotato breeding as well as in other autopolyploids.
Pathways for Improving Inpatient Pediatric Asthma Care (PIPA): A Multicenter, National Study
Authors: Kaiser Sunitha V. , Jennings Brittany , Rodean Jonathan , Cabana Michael D. , Garber Matthew D. , Ralston Shawn L. , Fassl Bernhard , Quinonez Ricardo , Mendoza Joanne C. , McCulloch Charles E. , Parikh Kavita 
Published: May 2020

BACKGROUND AND OBJECTIVES:
Pathways guide clinicians through evidence-based care of specific conditions. Pathways have been demonstrated to improve inpatient asthma care but mainly in studies at large, tertiary children’s hospitals. It remains unclear if these effects are generalizable across diverse hospital settings. Our objective was to improve inpatient asthma care by implementing pathways in a diverse, national sample of hospitals.

METHODS:
We used a learning collaborative model. Pathway implementation strategies included local champions, external facilitators and/or mentors, educational seminars, quality improvement methods, and audit and feedback. Outcomes included length of stay (LOS) (primary), early administration of metered-dose inhalers, screening for secondhand tobacco exposure and referral to cessation resources, and 7-day hospital readmissions or emergency revisits (balancing). Hospitals reviewed a sample of up to 20 charts per month of children ages 2 to 17 years who were admitted with a primary diagnosis of asthma (12 months before and 15 months after implementation). Analyses were done by using multilevel regression models with an interrupted time series approach, adjusting for patient characteristics.

RESULTS:
Eighty-five hospitals enrolled (40 children’s and 45 community); 68 (80%) completed the study (n = 12 013 admissions). Pathways were associated with increases in early administration of metered-dose inhalers (odds ratio: 1.18; 95% confidence interval [CI]: 1.14–1.22) and referral to smoking cessation resources (odds ratio: 1.93; 95% CI: 1.27–2.91) but no statistically significant changes in other outcomes, including LOS (rate ratio: 1.00; 95% CI: 0.96–1.06). Most hospitals (65%) improved in at least 1 outcome.

CONCLUSIONS:
Pathways did not significantly impact LOS but did improve quality of asthma care for children in a diverse, national group of hospitals.
Effects of vitamin pre-sowing treatment on sweet maize seedlings irrigated with saline water
Authors: Pradi Vendruscolo Eduardo , Seleguini Alexsander 
Published: May 2020

Salinity stress represents an obstacle for the production of plants of commercial interest, including sweet maize. Among the techniques used to suppress the effects caused by excess salts in the soil is the exogenous application of vitamins. Thus, the objective of this study was to evaluate the effect of the pre-sowing treatment of sweet maize seeds in solutions of thiamine, niacin and these two vitamins combined on the development of the plants irrigated with saline water. The treatments were composed by the 24 h pre-sowing treatment with water, thiamine solution (thiamine 100 mg L-1), niacin solution (niacin 100 mg L-1) and a combined solution with these two vitamins (thiamine 50 mg L-1 + niacin 50 mg L-1) irrigated with saline water. A control (pre-sowing treatment with water and irrigation with non-saline water) was also used. It was observed that salinity stress affects the sweet maize initial development. Application of isolate thiamine or niacin and its combined application improves the relative contents of chlorophyll ‘a’ and total, height of shoot, leaf number, diameter of stem and shoot, root and total dry matter, reducing dry matter losses by 8.89%, 25.46% and 39.60%, respectively. Soaking seeds for 24 h in thiamine and niacin solutions improve the initial growth of sweet maize plants under salt stress and a combined vitamin solution (thiamine 50 mg L-1 + niacin 50 mg L-1) effectively reduces the salt stress negative effect on the initial development of these plants.
Effects of humus and shading levels in the production of Lactuca canadensis L. seedlings
Authors: Pallaoro Dryelle Sifuentes , Arantes Cárita Rodrigues de Aquino , Correa Amanda Ribeiro , Camili Elisangela Clarete , Coelho Maria de Fátima Barbosa 
Published: May 2020

The objective of this study was to verify levels of shade and doses of earthworm humus in the production of Lactuca canadensis L. seedlings. The experimental design was completely randomized in a 4 x 5 factorial scheme (four levels of shade and five doses of humus). The different levels were obtained using black polyethylene screen with 35, 50 and 70% shade and in full sun. The substrates were obtained by mixing commercial substrate for vegetables (Vivatto Slim ® plus), with earthworm humus in the proportions of 0, 15, 30, 45 and 60%. There was no seedlings emergence in the full sun and, the treatment with 50% of shade allows the best development. The substrate without humus and with 60% presented superior results but the higher dose stood out for number of leaves (3.14) and chlorophyll content (32.9%), in 50% of shade. For the other variables, there was no difference between the commercial substrate and 60% of humus, so the commercial substrate can be used in the production of Lactuca canadensis L. seedlings, considering the ratio benefit/cost ratio.
Effect of Bacillus subtilis and Bacillus amyloliquefaciens culture on the growth and yield of off-season potato (Solanum tuberosum L.)
Authors: Kantar Faik , Uysal Aysel 
Published: May 2020

A study was carried out in order to investigate the effect of microbial fertilizer application on off-season potato (Solanum tuberosum L. cv. Universia) under field conditions in early spring and late autumn in 2016. The experiment included Control (C, no application), Standard Fertilizer Application (SFA), Microbial Fertilizer Application (MFA) and SFA+MFA treatments. An equal mixture of three strains of Bacillus subtilis VKPM B-10641(DSM 24613), Bacillus amyloliquefaciens VKPM B-10642 (DSM 24614) and Bacillus amyloliquefaciens В-10643 (DSM 24615) was applied at 1x109 cfu/ml to tubers before planting and to plants at flowering stage in MFA and SFA+MFA plots. Treatments affected the plant growth parameters. SFA plots produced the highest tuber yields in both seasons. MFA plots had earlier emergence time (40 days), higher number of stems per plant (3), higher number of leaves per plant (37.2), thicker stem diameter (10.15 mm), higher above ground biomass yields (5.42 t/ha), higher single tuber weight (104.21 g) and higher tuber yields (22.06 t/ha) compared with control plots (42 days, 2.2, 31.2, 9.15 mm, 4.40 t/ha, 90.88 g and 20.14 t/ha respectively). Tuber yield in MFA plots (26.56 t/ha) was equal to SFA plots (26.81 t/ha) in warmer autumn planting. Combination of SFA and MFA produced lower tuber yields (21.72 t/ha) than SFA treatment (26.81 t/ha). In conclusion, Bacillus subtilis and Bacillus amyloliquefaciens gave comparable tuber yields to chemical fertilizer application in warmer autumn plantings, but lower tuber yields in colder spring conditions warranting further experiments with cold tolerant psychrophilic bacterial strains for off-season potato production.
Plant growth and yield of butter kale (Brassica oleracea L. var. acephala), as influenced by the combined application of bovine manure and rock powder
Authors: Micaela Micaela B. Pereira , Dias Thiago Jardelino , Neriane Neriane Rodrigues de Lima , Evandra Evandra Da Silva Justino , Daivyd Daivyd S. Oliveira , Mario Mario L. Martins Veras 
Published: May 2020

The supply of organic matter in soil of arid and semiarid in Brazilian regions is very import, as it allows to increase crop yields. However, there are few studies on alternative sources of fertilizer for vegetables. Therefore, the objective was to evaluate the effect of different doses of bovine manure and rock powder (RP) on growth characteristics, concentration of chlorophyll, and physiological indices and production of (B. oleracea L. var. acephala). The treatments were arranged in five randomized blocks in a factorial 4 x 4 reference doses of bovine manure (60, 120, 180 and 240 g pit-1) combined with doses of rock powder (6, 12, 18 and 24 g pit-1). Doses of bovine manure and rock powder of 180 and 18 g, respectively, increased the growth of B. oleracea by plant height, stem diameter, leaf number. The combination doses of bovine manure 120 g + 12 g rock powder and 120 g of bovine manure + 18 g rock powder, provided, in this order, larger leaf area results for the productivity of B. oleracea. The bovine manure promotes greater concentration of CO2, net photosynthesis and stomatal conductance of B. oleracea plant, while rock powder limits these characteristics. Intermediate doses of bovine manure and RP promote higher growth and yield of B. oleracea.
La bitácora de diseño, artefacto cognitivo de aprendizaje. Externalización de modelos mentales y metacognición
Authors: Gonzalez-Tobon Juanita , Cuervo Roberto , Hernández Mihajlovic Edgar , Camacho Jorge 
Published: May 2020

La bitácora, un instrumento utilizado en los procesos de enseñanza-aprendizaje en diseño, suele utilizarse como registro del proyecto y no como un medio de externalización de los modelos mentales de los estudiantes y su transformación durante el curso de una asignatura. En este artículo de reflexión teórica se indaga por cómo la bitácora de diseño puede ser un artefacto cognitivo de aprendizaje para promover la metacognición en el proceso formativo. Para ello, se propone una aproximación cognitivista histórico-cultural en relación con el concepto de metacognición, como un aspecto fundamental para que se dé el aprendizaje en profundidad y comprender así la bitácora de diseño desde la noción de artefacto cognitivo, la cual se construye en las relaciones entre los agentes involucrados en el aula. Se establece el marco conceptual desde donde se formulan las categorías y posibles variables para comprender las representaciones externas que se presentan en la bitácora de diseño: sus niveles físico, simbólico y cognitivo. Las representaciones externas, como elementos simbólicos, son evidencia de los modelos mentales del sujeto y su aproximación a la solución de problemas desde el pensamiento diseñístico. Como resultado, se presenta una matriz con rasgos observables de las dimensiones y procesos metacognitivos.
Lexicalising phonological structure in morphemes
Authors: Nasukawa Kuniya 
Published: May 2020

AbstractBy comparing different theoretical models of phonological representation, this paper considers (i) what kinds of properties are lexically specified in morpheme-internal phonological structure, and (ii) how this morpheme-internal phonological structure is constructed before being stored in the mental lexicon. The aim is to contribute to the ongoing development of a model which can characterize the lexicalisation of phonological structure within morphemes.
Threadfin bream (Nemipteridae) resources in the sea of Vietnam based on the bottom trawl surveys
Authors: Anh Tran Nhat , Cuong Tran Van 
Published: May 2020

The threadfin bream (Nemipteridae) resources in the sea of Vietnam were analysed using the results of bottom trawl surveys conducted by the assessment of living marine resources project in Vietnam (ALMRV) in period 2000–2005 and comprehensive investigation of marine fisheries resources project in Vietnam sea during 2012–2013. Results showed that a total of 23 species belonging to 4 genera of Nemipterus, Parascolopsis, Pentapodus and Scolopsis were recorded. The Southeast region has the highest number of species encountered with 26 species, and the lowest number in Central waters with 16 species. The highest number of species was encountered at 50–100 m depth range with 25 species and the lowest number at the depth of less than 20 m with 9 species. Catch of threadfin bream comprised more than 1% of total catch of surveys. In threadfin bream family, genus Nemipterus was the most abundant, the catch of this genus contributed from 75.1% to 88.4% of the family catch. The catch per unit effort decreased from Northern region to Southern region, while the catch in the Northeast monsoon was higher than in the Southwest monsoon. The biomass of threadfin bream in Vietnamese water was estimated to be 7,534 to 12,229 tons depending on the monsoon patterns.
Delta-Like Protein 3 Expression and Targeting in Merkel Cell Carcinoma
Authors: Xie Hao , Kaye Frederic J. , Isse Kumiko , Sun Yan , Ramoth Johanna , French Dorothy M. , Flotte Thomas J. , Luo Yan , Saunders Laura R. , Mansfield Aaron S. 
Published: May 2020

Abstract

Purpose
Delta-like protein 3 (DLL3) is being developed as a predictive biomarker for DLL3-targeting antibody-drug conjugate and other therapies. Given the neuroendocrine features of Merkel cell carcinoma (MCC), we sought to evaluate DLL3 expression and its role in MCC.

Experimental Design
Formalin-fixed and paraffin-embedded MCC cases were consecutively selected. Immunohistochemistry was performed for DLL3 (SC16.65 antibody) and polyomavirus large T-antigen (sc-136172 antibody). Slides were read out for percentage of positive tumor cells. Cox proportional hazards model was applied to assess the association between DLL3 expression and overall survival (OS). A patient with a DLL3-expressing MCC was treated with rovalpituzumab tesirine (Rova-T) in the “other tumor” cohort of NCT02709889 and assessed for response.

Results
The median H-score of DLL3 expression of 65 patients included was 60 (interquartile range, 30–100). Fifty-eight cases (89%) had ≥1% tumor cells positive for DLL3 expression with any intensity, of which the median DLL3 expression was 50% (interquartile range, 25%–70%). Thirty-four cases (52%) had ≥50% tumor cells positive for DLL3 expression with any intensity. Higher H-score of DLL3 expression was associated with higher polyomavirus nuclear expression (p = .003) when it was dichotomized to negative versus positive. H-score of DLL3 expression did not predict OS of patients with MCC (p = .4) after being adjusted for common clinicopathological factors. A patient treated with Rova-T for refractory metastatic MCC achieved partial response.

Conclusions
DLL3 overexpression is very common in MCC by immunohistochemistry. The response to treatment suggests that DLL3 expression may have predictive relevance for DLL3-targeting therapies in MCC.

Implications for Practice
Delta-like protein 3 (DLL3) is being developed as a predictive biomarker to identify patients for treatment with DLL3-targeting agents. Merkel cell carcinoma (MCC) is an aggressive neuroendocrine carcinoma of the skin. It was found that DLL3 overexpression is very common in MCC by immunohistochemistry and significantly associated with Merkel cell polyomavirus expression. Despite the lack of prognostic significance in this cohort, DLL3 expression may have predictive relevance for DLL3-targeting therapies in MCC. The high levels of DLL3 expression in a subset of MCC may potentially be used to select patients to receive DLL3-targeting therapies.
Gülen Yüzlerin Ardından: Angelman Sendromu
Authors: AKCA Unal , SANRI Aslihan , AKÇA Gülfer , DOLU Merve Hilal , TAŞDEMİR Haydar Ali 
Published: May 2020

Amaç: Nörogelişimsel gerilik,
epilepsi nedeniyle polikliniğimize başvuran Angelman Sendromu kliniği taşıyan
hastalarımızın genetik ve klinik özelliklerini sunmak.

Yöntem:Angelman/ Prader-Willi Sendromu
tanısı koyduğumuz hastaların retrospektif incelenmesi.

Bulgular:Mikrosefalisi, sürekli gülme
atakları,hipotonisitesi,gelişimsel geriliği olan iki kız üç erkek hastamızın FISH incelemeleri 15q11-q13
delesyonu olarak sonuçlandı. Yaşları 1-4 yaş aralığındaydı. 3 hastamızın
epilepsisi mevcuttu. EEG’lerinde zemin aktivite bozukluğu ve diken dalgalardan
oluşan epileptik aktivite saptandı. Antiepileptiklerle kontrol altına
alındılar.Tüm hastalarda konuşma bozukluğu
mevcutken, en sık görülen dismorfik bulgu geniş ağız, sonrasında
sırasıyla frontalbossing, geniş aralıklı dişler ve burun kökü basıklığıydı.
Prader-Willi tanısı alan NG ile beslenen 1 yaşındaki erkek hastada antenatal polihidramnioz ve inmemiş testis operasyonu hikayesi
mevcuttu.Sık enfeksiyon geçiren bir hastada immün yetmezlik saptandı, İVİG
tedavisi alıyor. Kranial görüntülemede bir hastada terminal zon, bir hastada
corpuscallozum inceliği saptandı. Diğer hastaların görüntülemeleri normaldi. Görme ve işitme geriliği izlenmedi.
Tüm hastalarımız fizik tedavi ve konuşma terapisi almaktadır.

Sonuçlar:Angelman
Sendromu(AS) 15q11-q13 lokusunu içeren delesyon, uniparentaldizomi,
imprintlenmedefekti veya UBE3Agen defektinden kaynaklanan, nadir görülen bir
genetik hastalıktır. 15q11q13 bölge delesyonu, AS’ unun yaklaşık%70 inden
sorumludur. Etkilenmiş kişilerde şiddetli mental retardasyon, motor gelişimde
gerilik, konuşma bozukluğu, karakteristik yüz görünümü bulunmaktadır. 15q11-q13
delesyonlu kromozom anneden kalıtıldığında AS’u, babadan kalıtıldığındaPrader-Willi sendromu
oluşmaktadır
The Relationship of Intrauterine Growth Restriction with Placental Pathologic Changes in Newborns
Authors: Dalili Hossein , Nayeri Fatemeh Sadat , Mirjalili Seyed Reza , Hossein Seyyed Nasrollah , Abdollahi Alireza , Shariat Mamak , Hantoushzadeh Sedigheh 
Published: May 2020

Introduction: Intrauterine growth restriction is a multifaceted problem and is associated with a significant increase in the level of morbidity and perinatal mortality. According to some studies, failure of the placenta is responsible for the most cases of intrauterine growth restriction. The aim of this study was to evaluate the placental pathologic changes in the intrauterine growth restriction (IUGR) samples and compare them with normal cases.

Methods: A study population consisted of 60 intrauterine growth restriction neonates and 60 normalized neonates born at Tehran Imam Khomeini Hospital between June 2016 and July 2017. The placenta was weighed, immediately after delivery, and the umbilical cord was separated, then stored in 10% formalin and sent for pathological examination as soon as possible. Data collection was performed according to the following items: the pathologist's report, the results of the infants' examination, and the data in the neonatal cases.

Results: The intrauterine growth restriction group showed a high frequency of placenta infarction (P < 0.001), inflammation of the villous (P < 0.001), villous fibrosis (P = 0.044), villous vascularization disorder (P = 0.001), prevalence of chorioamnionitis (P = 0.027), prevalence of Syncytiotrophoblastic knots (P < 0.001) and placental necrosis (P = 0.048) than normal group. However, the mean weight of the placenta (P < 0.001), the length and width of the macroscopic placenta changes was less (P < 0.001).

Conclusion: The results of the current study showed that a major part of the macroscopic and histological changes are detectable in the intrauterine growth restriction samples, which are considerably more common than normal, although they are not pathognomonic, but in the future, more accurate results can be obtained from more extensive studies.
Investigating the Relationship between Resiliency and Psychological Well-Being of Nurses in ICU & NICU of University Hospitals of Shiraz in 2012
Authors: Sadeghifar Esmat , Salari Mehrdad , Farnia Frahnaz , Mirzae Samaneh , Nasiriani Khadijeh 
Published: May 2020

Background: Human resources, especially nurses, have a significant role in health care services due to the nature of their service and their direct relationships with patients. In this regard, nurses are encountered with higher stressful factors resulting from their responsibilities in providing ease and comfort for patients. Resiliency is one of the main purposes of health promotion and is considered to be a protective agent. Moreover, psychological well-being refers to striving for perfection in order to realize the potential and real individual talents and abilities. This study aims to determine the relationship between resiliency and psychological well-being of nurses in ICU & NICU of Shiraz in 2017.

Methods: This is a descriptive-correlational research. The statistical society consists of 150 nurses working in ICU & NICU of university hospitals in Shiraz. The tools of data collection were psychological well-being and resiliency questionnaires. The collected data were analyzed via SPSS software and descriptive-inferential statistics.

Results: According to the Table, 86% of the subjects are females, and 14% are males; 53.4% of them are single, and 44.6% of them are married; 84% of them have bachelor degree, and 16% of them have master degree. Average age and work experience of the subjects are 31.38 and 4.79 years, respectively. Average resiliency and psychological well-being scores of the nurses are estimated to be 66.03 ± 16.72 and 119.55 ± 24.35, respectively. Their significant relationship was approved by Pearson test P = 0.002).

Conclusions: The results indicated that resiliency and psychological well-being of the nurses have significant relationship; with an increase in resiliency, their psychological and mental health increases. Therefore, some programs and interventions are recommended for nurses in order to improve their resiliency, and consequently, promote their psychological well-being.