bluuebunny/crossref_metadata_2025_split huggingface dataset:
Darwin’s second ‘abominable mystery’: trait flexibility as the innovation leading to angiosperm diversity
Authors: Onstein Renske E. 
Published: October 2019

SummaryThe fact that angiosperms are so species‐rich and ecologically diverse – Darwin’s second abominable mystery – could be explained by their ability to ‘reinvent’ themselves by evolving functional traits repeatedly over time, space and taxonomic clades. These trait innovations may facilitate adaptation and increase diversification rates. In this article, I quantify this ‘trait flexibility’ by reviewing the literature on trait transition rates and trait‐dependent diversification rates in angiosperms and their extant sister clade, acrogymnosperms. I show that angiosperms indeed evolved elevated trait transition and trait‐dependent diversification rates compared to gymnosperms, and rates are highest within species‐rich angiosperm orders (e.g. Fabales, Lamiales). The (genetic) ability of certain angiosperm lineages to repeatedly evolve adaptive traits may have facilitated sustained high net diversification resulting from numerous episodic radiations.
Functional analysis of hisJ in Aeromonas veronii reveals a key role in virulence
Authors: Zhang Hai‐peng , Kang Yuan‐huan , Kong Ling‐cong , Ju An‐qi , Wang Yi‐ming , Muhammad Inam , Zhang Dong‐xing , Qian Ai‐dong , Shan Xiao‐feng , Ma Hong‐xia 
Published: October 2019

AbstractAeromonas veronii is an important aquatic zoonotic pathogen in humans and animals. In recent years, extracellular proteins from bacteria have been found to be the major pathogenic factors for aquatic animals. The aim of this study was to systematically analyze the extracellular proteins of nine sources of A. veronii and the effects of hisJ on virulence. We screened only the common proteins from nine different sources of A. veronii by liquid chromatography−tandem mass spectrometry and identified the gene hisJ. We then constructed ΔhisJ (deleted) and C‐hisJ (complemented) variants of A. veronii TH0426 to assess the biological function of hisJ. While the ΔhisJ strain did not show altered growth (P > 0.05), we observed that it had reduced colony formation and biofilm formation and reduced adhesion to and invasion of epithelioma papulosum cyprini cells by 2.0‐, 1.9‐, and 10.8‐fold, respectively. Additionally, infection experiments on zebrafish and mouse infection experiments showed that the virulence of the ΔhisJ strain was decreased by 865‐fold (P < 0.001) compared with the wild‐type strain; virulence of the complemented C‐hisJ strain was reduced only 2.8‐fold. Furthermore, in the context of hisJ deletion, flagella of A. veronii TH0426 were easily detached and the expression of virulence genes was downregulated. A persistence test (of bacterial colonies in crucian carp) showed that the number of bacteria in the immune organs of the ΔhisJ‐infected group was lower than that in the wild‐type–infected group. Overall, these results show that hisJ affects flagellar shedding, virulence, biofilm formation, adhesion, and invasion of A. veronii TH0426, and that hisJ is closely associated with virulence and plays a crucial role in its pathogenicity of A. veronii TH0426.
Relationships between Morphological Changes of Lower Limbs and Gender During Medial Compartment Knee Osteoarthritis
Authors: Lu Yang , Zheng Zhan‐le , Lv Ji , Hao Rui‐zheng , Yang Yi‐ping , Zhang Ying‐ze 
Published: October 2019

ObjectivesTo evaluate the dynamic changes of key morphology indicators of the lower extremities in the coronal plane with progressing medial compartment knee osteoarthritis (KOA) with an emphasis on gender‐dependent regional differences.MethodsThe radiographs of patients with non‐traumatic knee pain and varying degrees of genu varus were reviewed. Radiographs were studied in 1538 lower limbs of 883 consecutive patients who visited our hospital from January to July 2017; all patients had long‐standing anteroposterior image‐splicing radiographs taken of their lower limbs. Morphological indicators of bones and joints that can change the alignment of lower limbs or reflect cartilage wear and soft‐tissue relaxation were selected and measured with the help of picture archiving and communication systems. After comparing the data of different genders, the data of males and females was separated into three age groups, <40 years, 40–60 years, >60 years respectively, and then compared among age groups using the Kruskal‐Wallis and Mann–Whitney U tests. Scatterplots of age and all the measurements were drawn to determine the strength of the relations. The Pearson correlation test was performed to reveal correlations of measurements and age.ResultsFemoral bowing angle (FBA) and joint line convergence angle (JLCA) have obvious differences between different genders (P = 0.001, 0.000, respectively). This suggests that females have greater femoral curvature and joint space angle than males. Significant differences were found in hip‐knee‐ankle angle (HKA), FBA, distal femoral valgus resection angle (DFVRA), medial proximal tibial angle (MPTA), JLCA, and minimum joint space width (min‐JSW) by age groups in females (P = 0.000, 0.000, 0.000, 0.000, 0.003, 0.002, respectively). The difference of mechanical medial distal femoral angle (mMDFA) was significant with P values less than 0.05 deemed significant (P = 0.030). Significant correlations were found between age and all measurements (r = −0.166, 0.253, 0.270, −0.147, 0.089, −0.105, −0.076, respectively, P < 0.01). Whereas, the difference in min‐JSW by age group was the only significant one in males (P = 0.001), and no significant correlation was found between age and measurements (r = −0.107, 0.041, 0.134, −0.067, 0.079, −0.134, −0.098, respectively, P > 0.01).ConclusionsAs KOA progressed, both dynamic deformation of lower extremities and degeneration of articular cartilage could be found in females, while no obvious dynamic deformations were found in males. Dynamic deformation of lower extremities was the important feature and the major causative factor of KOA in females.
Genomic analysis reveals low tumor mutation burden which may be associated with GNAQ/11 alteration in a series of primary leptomeningeal melanomas
Authors: Fortin Ensign Shannon , Bollin Kathryn , Millis Sherri Z. , Hinds Brian R. , Kosty Michael , Uchiyama Christopher 
Published: October 2019

AbstractPrimary central nervous system melanoma is rare and characterized by a variable prognosis, and no current treatment guidelines exist. We describe the clinical course of a 70‐year‐old female patient diagnosed with primary leptomeningeal melanoma (LMN) whose case represents the diagnostic and management challenges of this tumor. Targeted genomic sequencing of 315 genes from this tumor revealed GNAQ Q209L mutation and low (4 mutations/Megabase) tumor mutation burden (TMB). Wild‐type NRAS, KIT, and BRAF were also observed. A cohort of 4,787 melanomas was subsequently analyzed to identify additional primary central nervous system melanomas, of which 10 additional tumors met pathologic criteria (0.21% of total melanoma cohort). These tumors were genomically assessed according to the same targeted sequencing panel, and 6 of the tumors were also found to harbor a GNAQ mutation. All 10 tumors had low (less than or equal to 2 mutations/Megabase) TMB indicating a potential trend between G‐protein‐coupled receptor (GPCR) alterations and low TMB in LMNs. GPCR alterations were found to significantly correlate with TMB across the cohort of 4,787 melanomas, supporting this potential finding in the limited LMN subset.
Yoga therapy as an adjunct to traditional tooth brushing training methods in children with autism spectrum disorder
Authors: Ramassamy Eswari , Gajula Shivashankarappa Prathima , Adimoulame Sanguida , Meena Ramanathan , Elangovan Harikrishnan , Govindasamy Ezhumalai 
Published: October 2019

AbstractAimTo evaluate if yoga could be an adjunct to regular training methods in training brushing skill to children with autism spectrum disorder (ASD).MethodsSeventy‐two children with ASD aged 7‐15 years were selected and divided into two groups (N = 36). Children in Group I received visual pedagogy and video modeling and children in Group II received visual pedagogy and video modeling with yoga. Plaque and gingival indices (PI and GI) were recorded at baseline and at the end of first, second, third, and sixth month. The scores were summarized as mean and standard deviation and inter‐group comparison was done using independent t‐test.ResultsInter‐group comparison of mean plaque and gingival indices scores were statistically significant at second month (P = .039 for PI and P = .009 for GI). The scores were statistically significant even at third month (P = .001 for PI and P = .002 for GI) and sixth month (P = .001 PI and GI), with children in Group II demonstrating better oral hygiene.ConclusionYoga training can be used as an adjunct to enhance tooth brushing learning capabilities of children with ASD in addition to visual modeling and pedagogy.
Multiple‐output quantile regression through optimal quantization
Authors: Charlier Isabelle , Paindaveine Davy , Saracco Jérôme 
Published: October 2019

AbstractA new nonparametric quantile regression method based on the concept of optimal quantization was developed recently and was showed to provide estimators that often dominate their classical, kernel‐type, competitors. In the present work, we extend this method to multiple‐output regression problems. We show how quantization allows approximating population multiple‐output regression quantiles based on halfspace depth. We prove that this approximation becomes arbitrarily accurate as the size of the quantization grid goes to infinity. We also derive a weak consistency result for a sample version of the proposed regression quantiles. Through simulations, we compare the performances of our estimators with (local constant and local bilinear) kernel competitors. The results reveal that the proposed quantization‐based estimators, which are local constant in nature, outperform their kernel counterparts and even often dominate their local bilinear kernel competitors. The various approaches are also compared on artificial and real data.
Mutations in the miR396 binding site of the growth‐regulating factor gene VvGRF4 modulate inflorescence architecture in grapevine
Authors: Rossmann Susanne , Richter Robert , Sun Hequan , Schneeberger Korbinian , Töpfer Reinhard , Zyprian Eva , Theres Klaus 
Published: October 2019

SummaryBunch rot caused by Botrytis cinerea infections is a notorious problem in grapevine cultivation. To produce high quality fruits, grapevine plants are treated with fungicides, which is cost intensive and harmful to the environment. Conversely, loose cluster bunches show a considerably enhanced physical resilience to bunch diseases. With the aim to identify genetic determinants that modulate the development of bunch architecture, we have compared loose and compact 'Pinot noir' clones. Loose cluster architecture was found to be correlated with increased berry size, elongated rachis and elongated pedicels. Using transcriptome analysis in combination with whole genome sequencing, we have identified a growth‐regulating factor gene, VvGRF4, upregulated and harbours heterozygous mutations in the loose cluster clones. At late stages of inflorescence development, the mRNA pools of loose cluster clones contain predominantly mRNAs derived from the mutated alleles, which are resistant to miR396 degradation. Expression of the VvGRF4 gene and its mutated variants in Arabidopsis demonstrates that it promotes pedicel elongation. Taken together, VvGRF4 modulates bunch architecture in grapevine 'Pinot noir' clones. This trait can be introduced into other cultivars using marker‐assisted breeding or CRISPR‐Cas9 technology. Related growth‐regulating factors or other genes of the same pathway may have similar functions.
Plasma membrane integrity and oxidative stress index outcomes of canine progenitor epidermal keratinocytes (CPEKs) exposed to virgin coconut oil (VCO)
Authors: Boyd Megan , Morris Jordan M. , Santoro Domenico 
Published: October 2019

BackgroundThere is a rapidly growing market for topical use of virgin coconut oil (VCO). Studies of topical use in dogs are lacking.Hypothesis/ObjectiveThe objective of this study was to measure the release of lactate dehydrogenase (LDH, a plasma membrane disruption marker) and production of nitrite (Griess reaction, an oxidative stress marker) from a canine keratinocyte cell line after exposure to VCO as an initial toxicity screening to suggest future studies.Methods and materialsCanine progenitor epidermal keratinocytes (CPEKs) were plated onto permeable transwell membranes and cultured with undiluted organic VCO or control media. Following a 24 h incubation, an LDH assay and a Griess reaction were performed on the collected subnatants.ResultsExposure of CPEKs to VCO significantly increased LDH release compared to controls, 62.29 ± 16.32% versus 8.88 ± 5.82% (P = 0.0056) and there was no significant difference in production of nitrite compared to controls, 2.47 ± 1.56 μmol/L versus 1.42 ± 0.95 μmol/L (P = 0.086).Conclusions and clinical importanceBased on this study VCO induced an increased disruption of plasma membrane integrity, as measured by LDH. However, VCO did not induce increased oxidative stress, as measured by nitrite production. Based on these preliminary data, further studies to assess the toxicity of VCO are needed.
On Selberg’s eigenvalue conjecture for moduli spaces of abelian differentials
Authors: Magee Michael 
Published: October 2019

J.-C. Yoccoz proposed a natural extension of Selberg’s eigenvalue conjecture to moduli spaces of abelian differentials. We prove an approximation to this conjecture. This gives a qualitative generalization of Selberg’s $\frac{3}{16}$ theorem to moduli spaces of abelian differentials on surfaces of genus ${\geqslant}2$.
Performance Analysis of Photovoltaic-Heat Pump (PV/T) Combined Systems: A Comparative Numerical Study
Authors: Ozakin Ahmet Numan , Yakut Kenan , Khalaji Mansour Nasiri 
Published: October 2019

Abstract
In this study, the performance of the combined system of photovoltaic-heat pump (PV/T) and effects of a newly designed evaporator on the performance of the combined system were numerically investigated. The coefficient of performance (COP) value of the heat pump and electrical efficiency of the photovoltaic system were determined considering the studies carried out at the same resource temperature. ansys Fluent software was used in the numerical analysis. According to the results, the electrical efficiency increased as expected. The maximum electrical efficiency obtained for photovoltaic system was 2.5% higher than those without cooling systems and the COP of the heat pump system was determined to be 4.75 at its maximum. The maximum exergetic efficiency of the combined system was calculated to be 55.5% at 6 m/s of refrigerant velocity.
Eruption age and duration of the ∼9 km3 Burney Mountain dacite dome complex, northern California, USA
Authors: Downs Drew T. , Clynne Michael A. , Champion Duane E. , Muffler L.J. Patrick 
Published: October 2019

Abstract
At ∼9 km3, the six dacite domes (db1–db6) of Burney Mountain, northern California, USA, constitute the most voluminous Quaternary dome complex in the Cascades volcanic arc. Whole-rock geochemistry, electron microprobe, and petrographic data indicate that the domes are magmatically related, which when integrated with geomorphology and stratigraphic superposition, indicate early (db1, db2, and db3) and late (db4, db5, and db6) erupted groups. We present 40Ar/39Ar ages of 271.9 ± 4.6 ka (db1), 280.8 ± 8.2 and 281.7 ± 6.8 ka (db2), and 290.2 ± 6.0 ka (db3) along with a previous age of 280 ± 12 ka (db1). These ages scatter over 20 k.y., whereas remanent magnetic directions are similar between 53.3–59.0° inclination and 352.7–355.9° declination. The latter data set indicates that the dacite domes were emplaced over a geologically brief time interval, not thousands of years. Crystal-size distribution patterns of plagioclase were used to calculate residence times, which we use to infer the duration over which the eruptions likely occurred. Three slopes represent three populations of plagioclase crystals (fine-grained groundmass, coarse-grained groundmass, and phenocrysts). A commonly used growth rate for plagioclase in dacitic magmas (10−10 mm/s) yields 9–10 yr of growth for the coarse-grained groundmass (early erupted domes of db1, db2, and db3), whereas plagioclase in the fine-grained groundmass (late erupted domes of db4, db5, and db6) grew over 4–5 yr. All plagioclase phenocrysts have apparent residence times of 26–36 yr; however, they contain high anorthite (An)&gt;70 resorbed cores with sieve textures, which have euhedral, lower An&lt;65 overgrowth rims. Similarities in chemistry between groundmass plagioclase and phenocryst overgrowth rims indicate that they grew concurrently, and we therefore propose that both have similar residence times. Thus, the Burney Mountain dacite dome complex was emplaced during a single eruptive episode over the course of years to decades at 281.1 ± 4.8 ka (weighted mean age).
Behavioural difficulties in early childhood and risk of adolescent injury
Authors: Bandyopadhyay Amrita , Tingay Karen , Akbari Ashley , Griffiths Lucy , Bedford Helen , Cortina-Borja Mario , Walton Suzanne , Dezateux Carol , Lyons Ronan A , Brophy Sinead 
Published: October 2019

ObjectiveTo evaluate long-term associations between early childhood hyperactivity and conduct problems (CP), measured using Strengths and Difficulties Questionnaire (SDQ) and risk of injury in early adolescence.DesignData linkage between a longitudinal birth cohort and routinely collected electronic health records.SettingConsenting Millennium Cohort Study (MCS) participants residing in Wales and Scotland.Patients3119 children who participated in the age 5 MCS interview.Main outcome measuresChildren with parent-reported SDQ scores were linked with hospital admission and Accident & Emergency (A&E) department records for injuries between ages 9 and 14 years. Negative binomial regression models adjusting for number of people in the household, lone parent, residential area, household poverty, maternal age and academic qualification, child sex, physical activity level and country of interview were fitted in the models.Results46% of children attended A&E or were admitted to hospital for injury, and 11% had high/abnormal scores for hyperactivity and CP. High/abnormal or borderline hyperactivity were not significantly associated with risk of injury, incidence rate ratio (IRR) with 95% CI of the high/abnormal and borderline were 0.92 (95% CI 0.74 to 1.14) and 1.16 (95% CI 0.88 to 1.52), respectively. Children with borderline CP had higher injury rates compared with those without CP (IRR 1.31, 95% CI 1.09 to 1.57).ConclusionsChildren with high/abnormal hyperactivity or CP scores were not at increased risk of injury; however, those with borderline CP had higher injury rates. Further research is needed to understand if those with difficulties receive treatment and support, which may reduce the likelihood of injuries.
Incidence of necrotising enterocolitis before and after introducing routine prophylacticLactobacillusandBifidobacteriumprobiotics
Authors: Robertson Claire , Savva George M , Clapuci Raducu , Jones Jacqueline , Maimouni Hassan , Brown Eleanor , Minocha Ashish , Hall Lindsay J , Clarke Paul 
Published: October 2019

ObjectiveTo compare rates of necrotising enterocolitis (NEC), late-onset sepsis, and mortality in 5-year epochs before and after implementation of routine daily multistrain probiotics administration in high-risk neonates.DesignSingle-centre retrospective observational study over the 10-year period from 1 January 2008 to 31 December 2017.SettingLevel 3 neonatal intensive care unit (NICU) of the Norfolk and Norwich University Hospital, UK.PatientsPreterm neonates at high risk of NEC: admitted to NICU within 3 days of birth at <32 weeks’ gestation or at 32–36 weeks’ gestation and of birth weight <1500 g.InterventionPrior to 1 January 2013 probiotics were not used. Thereafter, dual-speciesLactobacillus acidophilusandBifidobacterium bifidumcombination probiotics were routinely administered daily to high-risk neonates; from April 2016 triple-species probiotics (L. acidophilus, B. bifidum,andB. longumsubspeciesinfantis) were used.Main outcome measuresIncidence of NEC (modified Bell’s stage 2a or greater), late-onset sepsis, and mortality.ResultsRates of NEC fell from 7.5% (35/469 neonates) in the pre-implementation epoch to 3.1% (16/513 neonates) in the routine probiotics epoch (adjusted sub-hazard ratio=0.44, 95% CI 0.23 to 0.85, p=0.014). The more than halving of NEC rates after probiotics introduction was independent of any measured covariates, including breast milk feeding rates. Cases of late-onset sepsis fell from 106/469 (22.6%) to 59/513 (11.5%) (p<0.0001), and there was no episode of sepsis due toLactobacillusorBifidobacterium. All-cause mortality also fell in the routine probiotics epoch, from 67/469 (14.3%) to 47/513 (9.2%), although this was not statistically significant after multivariable adjustment (adjusted sub-hazard ratio=0.74, 95% CI 0.49 to 1.12, p=0.155).ConclusionsAdministration of multispeciesLactobacillusandBifidobacteriumprobiotics has been associated with a significantly decreased risk of NEC and late-onset sepsis in our neonatal unit, and no safety issues. Our data are consistent with routine use ofLactobacillusandBifidobacteriumcombination probiotics having a beneficial effect on NEC prevention in very preterm neonates.
Parents of babies who participated in an invasive clinical study report a positive experience: the Glucose in Well Babies (GLOW) study
Authors: Cumberpatch Alana R , Weston Philip J , Harding Jane E , Harris Deborah L 
Published: October 2019

ObjectiveThere is a paucity of data about normal blood metabolite concentrations in healthy babies, in part because of a reluctance to undertake non-therapeutic invasive testing in newborns. The Glucose in Well Babies study (GLOW) sought to describe blood glucose, lactate and beta-hydroxybutyrate concentrations in healthy term babies over the first 5 postnatal days. We also sought to understand both parents’ experience of participation in this invasive non-therapeutic study.Design, setting, patients and interventionsEligible babies were healthy, term, appropriately grown singletons born in a birthing centre, hospital or home within the greater Hamilton area and then discharged home. Babies had subcutaneous continuous glucose monitoring placed soon after birth, up to 14 heel-prick blood samples, twice-daily home visits and parents were asked to record all feeds. At study completion, both parents were asked to independently complete a questionnaire about their experience.ResultsAll eligible babies completed the study and every parent completed the questionnaire (65 fathers, 66 mothers). Parents reported they liked contributing to improving healthcare (126/131, 96%) and support from the GLOW team (119/131, 91%). Nearly all (127/131, 97%) would participate in GLOW again if they had another eligible baby, and all would recommend GLOW to family and friends. Two-thirds of parents (87/131, 66%) reported that participation had made them more likely to contribute to clinical research in the future.ConclusionsNon-therapeutic studies involving invasive procedures in healthy term babies are feasible, and parents were positive about their experience.