bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengaruh Perilaku Personal Hygiene Petugas Instalasi Gizi Terhadap Angka Kuman Tangan
Authors: Rodhi Inayati Habib 
Published: June 2020

Pelayanan gizi di Rumah sakit merupakan pelayanan penunjang untuk proses penyembuhan pasien. Personal hygiene atau kebersihan pengelola makanan merupakan prosedur menjaga kebersihan makanan yang sehat dan aman. Tahun 2002 terdapat 5 - 6 kasus infeksi nosokomial setiap 100 kunjungan rumah sakit, salah satu penyebabnya keracunan makanan akibat perilaku personal hygiene petugas instalasi gizi yang buruk. Penelitian bertujuan mengetahui pengaruh perilaku personal hygiene terhadap angka kuman petugas instalasi gizi. Metode penelitian observasional analitik dengan desain cross sectional pada 13 petugas Instalasi Gizi di RS PKU Muhammadiyah Gamping. Pengumpulan data menggunakan check list dan uji laboratorium mengukur jumlah angka kuman. Analisis data uji Chi-square untuk mengetahui pengaruh antar variabel perilaku personal hygiene dan angka kuman tangan. Tingkat perilaku personal hygiene petugas instalasi gizi 53,85% berkategori sedang dan 46,15% berkategori baik. Angka kuman tangan petugas instalasi gizi 61,54% melebihi standar angka kuman yang diperbolehkan dan 38,46% kurang dari standar. Angka kuman yang tertinggi 3000 CFU/cm2 dan yang terendah 50 CFU/cm2. Perilaku personal hygiene tidak mempengaruhi angka kuman tangan (p>0,05). Kesimpulan tidak terdapat pengaruh perilaku personal hygiene terhadap angka kuman tangan petugas instalasi gizi di RS PKU Muhammadiyah Gamping yang signifikan.
FAKTOR YANG MEMPENGARUHI KONSUMSI SAYUR DAN BUAH REMAJA DI SMP NEGERI 238 JAKARTA
Authors: Amelia Chika Meidiana , Fayasari Adhila 
Published: June 2020

Vegetables and fruit are generally food groups that are consumed by many people because many contains of benefits. Even though, in Indonesia consumption of vegetables and fruit still less than recommendation. The purpose of this research is to identify the factors affecting the consumption of vegetables and fruit adolescent at SMP NEGERI 238 Jakarta. This research uses design cross sectional and sampling using system random sampling that gets 107 students class VII, VIII, and IX in January 2020. The data obtained is based on the result of the questionnaire, form SQ-FFQ and Recall 24hour. Statistic data analysis used chi-square and logistic regression test. The result of this study indicate that adolescent who consumed vegetables and fruit according to recommendations per day are 24,3%. Bivariate result indicate there were significant relationship between self efficacy (p = 0,034), availability vegetables and fruit (p = 0,048), parental influence (p = 0,032), and there is no significant relationship between knowledge (p= 0,063). Multivariate result indicate there were signification relationship between knowledge, self efficacy, availability vegetables and fruit, and parental influence to consumption of vegetables and fruit, with highest OR value 5,634 on parental influence.
THE ROLE OF INDICTMENT OF PUBLIC PROSECUTORIN ERADICATION OF THE CASE OF CORRUPTIONIN INDONESIAN CRIMINAL JUSTICE SYSTEM
Authors: Yanto Oksidelfa , Rusdiana Erma , Sari Nani Widya , Lestari Yulita Puji 
Published: June 2020

AbstrakPenelitian ini bertujuan untuk mengetahui peran kejaksaan sebagai lembaga penegak hukum bidang penuntutan dan juga ingin mengetahui peran surat dakwaan jaksa penuntut umum dalam penegakan hukum kejahatan korupsi dalam sistem peradilan pidana Indonesia. Metode yang penulis gunakan dalam penulisan ini adalah metode penelitian hukum yuridis normatif dengan menggunakan data sekunder yang berasal dari bahan-bahan hukum yang relevan dengan masalah yang diteliti. Hasil penelitian menunjukkan bahwa peran jaksa sebagai lembaga penegak hukum bidang penuntutan yang diberi kewenangan membuat surat dakwaan khususnya dalam hal pemberantasan korupsi memiliki arti yang sangat penting dalam mewujudkan tercapainya tujuan hukum. Hal ini dikarenakan jaksa merupakan bagian dari sub sistem peradilan pidana yang ada di Indonesia. Sebagai sub sistem jaksa memiliki kewenangan dalam hal pemberantasan korupsi dengan memberikan tuntutan pidana paling berat kepada pelaku korupsi melalui surat dakwaannya.”
Model Construction of Medical Endoscope Service Evaluation System-Based on the Analysis of Delphi Method
Authors: Zheng Jun , Lou Ligang , Xie Ying , Chen Siyao , Li Jun , Wei Jingming , Feng Jingyi 
Published: June 2020

Abstract
Background: Medical endoscope is widely used in clinical practice for the purpose of diagnosis and treatment，occupying around 5% of the medical device market. Evaluating the true service level of medical endoscope is essential and necessary to improve overall performance of medical diagnosis and treatment, and to maintain competitiveness of endoscope manufacturers, however, such a tool is not available in the market. This study develops an Evaluation Index System (EIS) to assess service level of medical endoscope, and to provide suggestions for improving the service level through the Delphi method.Methods: Firstly, the possible factors influencing the service level were identified from literature review. In parallel, the Delphi expert method questionnaire was designed and 25 experts were invited to conduct three rounds of questionnaire, to evaluate and rate the possible factors. Finally, we determined the weights associated with the factors, using the analytic hierarchy process (AHP) and percentage method, and developed the service level EIS.Results: The EIS consists of 3 first-level indicators, 24 second-level indicators and 68 third-level indicators. According to the weights computed using AHP, first-level indicators are ranked as post-sale (0.62), in-sale (0.25) and pre-sale (0.13). Through case verification, the medical endoscope brand Olympus had a total score of 4.17, Shanghai Aohua had a total score of 3.71, and Shanghai Chengyun had a total score of 3.28, which matches its market popularity and ranking in terms of market share. The results obtained from the EIS are consistent with the reality.Conclusions: The EIS established in this study is comprehensive, reliable and reasonable with strong practicality. The EIS can act as a tool for the endoscope users to evaluate potential products and make informed choices. It also provides a measurable basis for endoscope manufacturers and service providers to improve service quality.
Comparative transcriptome analysis of root, stem, and leaf tissues of Entada phaseoloides reveals potential genes involved in triterpenoid saponin biosynthesis
Authors: Liao Weifang , Mei Zhinan , Miao Lihong , Liu Pulin , Gao Ruijie 
Published: June 2020

Abstract
Background: Entada phaseoloides (L.) Merr. is an important traditional medicinal plant. The stem of Entada phaseoloides is popularly used as traditional medicine because of its significance in dispelling wind and dampness and remarkable anti-inflammatory activities. Triterpenoid saponins are the major bioactive compounds of Entada phaseoloides. However, genomic or transcriptomic technologies have not been used to study the triterpenoid saponin biosynthetic pathway in this plant.Results: We performed comparative transcriptome analysis of the root, stem, and leaf tissues of Entada phaseoloides with three independent biological replicates and obtained a total of 53.26 Gb clean data and 116,910 unigenes, with an average N50 length of 1218 bp. Putative functions could be annotated to 42,191 unigenes (36.1%) based on BLASTx searches against the Non-redundant, Uniprot, KEGG, Pfam, GO, KEGG and COG databases. Most of the unigenes related to triterpenoid saponin backbone biosynthesis were specifically upregulated in the stem. A total of 26 cytochrome P450 and 17 uridine diphosphate glycosyltransferase candidate genes related to triterpenoid saponin biosynthesis were identified. The differential expressions of selected genes were further verified by qPT-PCR. Conclusions: The dataset reported here will facilitate the research about the functional genomics of triterpenoid saponin biosynthesis and genetic engineering of Entada phaseoloides.
Oncolytic vaccinia virus GLV-1h68 exhibits profound antitumoral activities in cell lines originating from neuroendocrine neoplasms
Authors: Kloker Linus D , Berchtold Susanne , Smirnow Irina , Beil Julia , Krieg Andreas , Sipos Bence , Lauer Ulrich M 
Published: June 2020

Abstract
Background: Oncolytic virotherapy is an upcoming treatment option for many tumor entities. But so far, a first oncolytic virus only was approved for advanced stages of malignant melanomas. Neuroendocrine tumors (NETs) constitute a heterogenous group of tumors arising from the neuroendocrine system at diverse anatomic sites. Due to often slow growth rates and (in most cases) endocrine non-functionality, NETs are often detected only in a progressed metastatic situation, where therapy options are still severely limited. So far, immunotherapies and especially immunovirotherapies are not established as novel treatment modalities for NETs.Methods: In this immunovirotherapy study, pancreatic NET (BON-1, QGP-1), lung NET (H727, UMC-11), as well as neuroendocrine carcinoma (NEC) cell lines (HROC-57, NEC-DUE1) were employed. The well characterized genetically engineered vaccinia virus GLV-1h68, which has already been investigated in various clinical trials, was chosen as virotherapeutical treatment modality. Results: Profound oncolytic efficiencies were found for NET/NEC tumor cells. Besides, NET/NEC tumor cell bound expression of GLV-1h68-encoded marker genes was observed also. Furthermore, a highly efficient production of viral progenies was detected by sequential virus quantifications. Moreover, the mTOR inhibitor everolimus, licensed for treatment of metastatic NETs, was not found to interfere with GLV-1h68 replication, making a combinatorial treatment of both feasible. Conclusions: In summary, the oncolytic vaccinia virus GLV-1h68 was found to exhibit promising antitumoral activities, replication capacities and a potential for future combinatorial approaches in cell lines originating from neuroendocrine neoplasms. Based on these preliminary findings, virotherapeutic effects now have to be further evaluated in animal models for treatment of Neuroendocrine neoplasms (NENs).
Incidence, risk factors and outcomes of early postoperative hyperglycemia in surgical patients: a protocol for a systematic review and meta-analysis
Authors: Ssentongo Paddy , Lewcun Joseph A. , Ssentongo Anna E. , Soybel David I. 
Published: June 2020

Abstract
Background: Early postoperative hyperglycemia (POHG) is common and associated with poor postoperative outcomes. Currently, there is no systematic review and meta-analysis that addresses the knowledge gap of the incidence of POHG in surgical patients and that explores the associated risk factors and complications. The objective of this study will be to estimate the pooled incidence, risk factors and clinical outcomes of early postoperative hyperglycemia in men and women globally. Methods: We designed and registered a study protocol for a systematic review and meta-analysis of studies reporting the incidence of postoperative hyperglycemia (POHG). We will search PubMed (MEDLINE), Scopus, Web of Science, EMBASE, Cochrane Library, OVID (HEALTH STAR), OVID (MEDLINE), Joana Briggs Institute EBF Database (from inception onwards). Randomized controlled trials and observational cohort studies reporting the incidence of POHG and conducted in surgical patients will be included. No age, geographical location, study-design or language limits will be applied. The primary outcome will be the incidence of POHG. Secondary outcomes will be risk factors and clinical outcomes of POHG. Two reviewers will independently screen citations, full text articles, and abstract data, extract data, and evaluate the quality and bias of included studies. Discrepancies will be resolved through discussion or consultation with a third researcher. The risk of bias and study methodological quality of included studies will be evaluated by the appropriate Cochrane risk of bias tool for randomized trials and Newcastle-Ottawa Scale for cohort studies. If feasible, we will conduct random effects meta-analysis with a logit transformation of proportions. We will report the probability of postoperative hyperglycemia as a measure of incidence rate, relative risk ratios (RR) and 95% confidence intervals to report the effects of the risk factors and postoperative outcomes. Additional analyses will be conducted to explore the potential sources of heterogeneity (e.g. age, gender, geographical location, publication year, comorbidities, type of surgical procedure). The Egger’s test and funnel plots will be used to assess small study effects (publication bias).Discussion This systematic review and meta-analysis will identify, evaluate and integrate the evidence on the incidence, risk factors and outcomes of early POHG in surgical patients. The results of this study can be used to identify populations which may be at particular risk for POHG. Future studies which use this information to better guide post-operative glycemic control in surgical patients could be considered.Systematic review registration: PROSPERO registration number CRD42020167138
Bloodstream Infections caused by Klebsiella pneumoniae and Serratia marcescens isolates co-harboring NDM-1 and KPC-2.
Authors: Bes Taniela , Nagano Debora , Martins Roberta , Marchi Ana Paula , Neto Lauro Perdigão , Higashino Hermes , Prado Gladys , Guimaraes Thais , Levin Anna S , Costa Silvia 
Published: June 2020

Abstract
Carbapenem-resistant Enterobacteriaceae is a worldwide health problem, however isolates carrying both blaKPC-2 and blaNDM-1 are unusual. Here we describe microbiological and clinical characteristics of five cases of bloodstream infection (BSI) caused by carbapenem-resistant Klebsiella pneumoniae and Serratia marcescens co-harboring blaKPC-2 and blaNDM-1.Of the five blood culture isolates, three from are from hematopoietic stem cell transplantation patients, one from a renal transplant patient, and one from a soft tissue surgical patient. All patients lived in low-income neighborhoods and had no travel history. Despite antibiotic treatment, four of five patients died. The phenotypic assays showed that Meropenem added with either EDTA, PA or both showed increased zone of inhibition in comparison to Meropenem alone. Molecular tests confirmed blaKPC-2 and blaNDM-1 genes, the K. pneumoniae were assigned as ST258 and ST340 by Whole Genome Sequencing.This case-series showed high mortality of BSI caused by Enterobacteria coproducing KPC-2 and NDM-1. The detection of samples co-harboring blaKPC-2 and blaNDM-1 remains unsatisfactory with phenotypic assay. Routine microbiology laboratories must be on alert for samples co-harboring these mechanisms.
Functional analysis of SlERF01 gene in the disease resistance to S.lycopersici
Authors: Yang Huanhuan , Shen Fengyi , Wang Hexuan , Zhao Tingting , Zhang He , Jiang Jingbin , Xu Xiangyang , LI JINGFU 
Published: June 2020

Abstract
Background: Tomato gray leaf spot disease caused by Stemphylium lycopersici ( S. lycopersici ) is a serious disease that can severely affect tomato production. So far, only resistance gene Sm has been reported and the molecular mechanism of tomato resistance to the disease remains unclear. To better understand this mechanism of tomato resistance to S. lycopersici , qRT-PCR analysis, physiological index determination, microscopic observation and transgenic technology were used in this study.
Results: Our results showed that SlERF01 could be strongly induced by S. lycopersici and the exogenous hormones salicylic acid (SA) and jasmonic acid (JA). Furthermore, overexpression of SlERF01 enhanced the hypersensitive response to S. lycopersici and elevated the expression of defense genes in tomato. Furthermore, the accumulation of lignin, callose and H 2 O 2 was increased in transgenic lines after inoculation with S. lycopersici. Here, our results showed that SlERF01 played an indispensable role in among multiple SA, JA and ROS signaling pathways to confer resistance to S. lycopersici invasion. Our findings also indicated that SlERF01 could activate PR1 gene expression and enhance resistance to S. lycopersici .
Conclusions: We identified SlERF01 , a novel tomato AP2/ERF transcription factor. The Functional verification demonstrated SlERF01 positively regulated tomato resistance to S. lycopersici . Our findings indicate that SlERF01 plays a key role in the multiple SA, JA and ROS signaling pathways to confer resistance to invasion by S. lycopersici. The findings of this study not only can help to better understanding of mechanisms of response to pathogens, but also will enable targeted breeding strategies for tomato resistance to S. lycopersici .
Comparative transcriptome analysis between inbred and hybrids reveals molecular insights into yield heterosis of upland cotton
Authors: Shahzad Kashif , Zhang Xuexian , Guo Liping , Qi Tingxiang , Bao Lisheng , Zhang Meng , Zhang Bingbing , Wang Hailin , Tang Huini , Qiao Xiuqin , Feng Juanjuan , Wu Jianyong , Xing Chaozhu 
Published: June 2020

Abstract
Background: Utilization of heterosis has greatly improved the productivity of many crops worldwide. Understanding the potential molecular mechanism about how hybridization produces superior yield in upland cotton is critical for efficient breeding programs. Results: In this study, high, medium, and low hybrids varying in the level of yield heterosis were screened based on field experimentation of different years and locations. Phenotypically, high hybrid produced a mean of 14% more seed cotton yield than its better parent. Whole-genome RNA sequencing of these hybrids and their four inbred parents was performed using different tissues of the squaring stage. Comparative transcriptomic differences in each hybrid parent triad revealed a higher percentage of differentially expressed genes (DEGs) in each tissue. Expression level dominance analysis identified majority of hybrids DEGs were biased towards parent like expressions. An array of DEGs involved in ATP and protein binding, membrane, cell wall, mitochondrion, and protein phosphorylation had more functional annotations in hybrids. Sugar metabolic and plant hormone signal transduction pathways were most enriched in each hybrid. Further, these two pathways had most mapped DEGs on known seed cotton yield QTLs. Integration of transcriptome, QTLs, and gene co-expression network analysis discovered genes Gh_A03G1024, Gh_D08G1440, Gh_A08G2210, Gh_A12G2183, Gh_D07G1312, Gh_D08G1467, Gh_A03G0889, Gh_A08G2199, and Gh_D05G0202 displayed a complex regulatory network of many interconnected genes. qRT-PCR of these DEGs was performed to ensure the accuracy of RNA-Seq data. Conclusions: Through genome-wide comparative transcriptome analysis, the current study identified nine key genes and pathways associated with biological process of yield heterosis in upland cotton. Our results and data resources provide novel insights and will be useful for dissecting the molecular mechanism of yield heterosis in cotton.
Effect of a free healthy school meal on fruit, vegetables and unhealthy snacks intake in Norwegian 10- to 12-year-old children
Authors: Vik Frøydis Nordgård , Heslien Kaia Elisabeth Paulsen , Lippevelde Wendy Van , Øverby Nina Cecilie 
Published: June 2020

Abstract
Background: Norwegian children have a lower intake of fruit, vegetables, and a higher intake of unhealthy snacks compared to dietary guidelines. Such dietary inadequacies may be detrimental for their current and future health. Schools are favorable settings to establish healthy eating practices. Still, no school meal arrangement is provided in Norway, and most children typically bring packed lunches from home. The aim of this study was to investigate whether serving a free healthy school meal for one year resulted in a higher intake of fruit and vegetables and a lower intake of unhealthy snacks in total among 10-12-year-olds in Norway.Methods: The School Meal Project in Southern Norway was a non-randomized trial in two elementary schools in rural areas in the school year 2014/2015. The study sample consisted of 10- to 12-year-old children; an intervention group (N=55) and a control group (N=109) resulting in a total of 164 school children at baseline. A food frequency questionnaire was completed by the children at baseline, at five months follow-up and after one year to assess fruit, vegetable and snacks intake. Multiple linear regression analyses were performed to assess intervention effects on overall intake of fruit and vegetables and unhealthy snacks.Results: Serving of a free healthy school meal for one year was associated with a higher weekly intake of vegetables on sandwiches in the intervention group compared to the control group, adjusted for baseline intake (B: 1.11 (95% CI: .38, 1.85)) at the end of the intervention. No other significant intervention effects were found for the remaining fruit and vegetables measures. Serving of a free healthy school meal was not associated with a lower weekly intake of unhealthy snacks (i.e. potato chips, candy and sugar sweetened beverages) in the intervention group compared to the control group. Conclusions: A free healthy school meal was associated with a higher weekly intake of vegetables on sandwiches but did not significantly change any other investigated dietary behaviors. However, given the inadequate intake of vegetables among children and that even moderate improvements have public health relevance, a free healthy school meal for all school children could be beneficial.
The effects of aerobic exercise training with octopamine supplementation on cardiomyocyte apoptosis induced by deep frying oil: the role of caspase and pro-Caspase 3
Authors: kianmehr Pantea , Azarbayjani Mohammad Ali , Peeri Maghsoud , Farzanegi Parvin 
Published: June 2020

Abstract
Background: Deep frying is a common cooking method, in which cooking is accompanied by carcinogenic by-products such as acrolein. Acrolein is a toxic byproduct of lipid peroxidation, which has been implicated in pulmonary, cardiac, and neurodegenerative diseases. The aim of this study was to explore the effect of aerobic exercise and octopamine on caspase 3 and pro-caspase 3 expression level in heart tissue of rat exposed deep frying oil.Methods: 30 male wistar rats were divided into 5 groups (n=6 in each) including 1) control (CO), 2) deep frying oil (DFO), 3) deep frying oil + exercise (DFO+EXE), 4) deep frying oil + octopamine (DFO+OCT), and 5) deep frying oil + exercise + octopamine (DFO+EXE+OCT). The apoptotic effects of DFO in heart tissue were examined by TUNEL assay. Masson's trichrome stain used to study cardiomyocytic fibers. Moreover, caspase 3 and pro-caspase 3 genes and proteins expression in all groups was evaluated using quantitative real-time PCR and western blotting method, respectively. Results: Data showed a significant increase in apoptotic cells in the DFO-treated group (P <0.05). In Masson's Trichrome stain analysis demonstrated more cardiomyocytic fibers degradation and aggregation lymphocytic cells in DFO that exercise and OCT signisicantly improve this degradation. Also, the expression level of caspase 3 and pro-caspase 3 was significantly decrease in deep frying oil + exercise + octopamine group (P <0.05). Conclusions: According to the result of the current study, it can be assumed that DFO can lead to programmed cell death via the activation of caspase in heart tissue. However, it seems that aerobic exercise with octopamine supplementation improve heart tissue by significantly decrease expression of caspase 3 and pro-caspase 3 that inhibit apoptosis.