bluuebunny/crossref_metadata_2025_split huggingface dataset:
T-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistanceT-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistance
Authors: Guan Yutao , Lin Yi , Zhang Fubin , Zhou Ling-ling , Chen Yang-ping , Zheng Xiaojiao , Cui Li-ning , Lin Zhong-qiu , Wang Gang 
Published: March 2020

Abstract
Background: T-cadherin plays a crucial role in maintaining normal tissue structure by regulating specific cell adhesion, cellular recognition and signal transduction. The purpose of this study was to evaluate whether T-cadherin influences epithelial ovarian cancer (EOC) progression, differentiation and drug resistance and its possible mechanisms.Methods: Epithelial ovarian carcinoma (EOC, n=63) and relevant contralateral normal ovarian (CNO, n=41) fresh tissues were collected from epithelial ovarian carcinoma patients, and benign ovarian tumour fresh tissues were collected from 55 patients with benign ovarian tumours. The human epithelial ovarian carcinoma cell line A2780 was cultured. T-cadherin expression was assessed by real-time RT-PCR and Western blotting, and the expression of matrix metalloproteinase-2 (MMP-2) was detected by Western blotting. pcDNA‑T‑cad plasmid technology was used to upregulate T-cadherin expression. In addition, A2780 cell migration and invasion ability, viability, colony formation, proliferation, apoptosis and sensitivity to paclitaxel were measured.Results: T‑cadherin mRNA and protein expression in EOC tissues from EOC patients was significantly downregulated, and there was no significant difference between the matched contralateral normal ovarian fresh tissue from the same patient and the benign ovarian tumour tissues from other patients. The migration and invasion abilities, viability, colony formation, and proliferation were attenuated by restoration of T‑cadherin expression in A2780 cells via pcDNA‑T‑cad transfection; apoptosis, MMP-2 expression and sensitivity to Taxol were also enhanced by restored T‑cadherin expression. The T‑cadherin expression level was well correlated with the clinical characteristics and symptoms of EOC patients, including tumour stage, histology, lymph node metastasis, tumour size, distant metastasis and cisplatin resistance.Conclusions: T‑cadherin participates in the processes of epithelial ovarian carcinoma cell migration, invasion, proliferation, apoptosis and sensitivity to paclitaxel and can regulate the expression of MMP-2. Downregulation of T‑cadherin expression may contribute to epithelial ovarian cancer progression, differentiation and drug resistance.
Thermal And Chemical Resistance Of Plasma Sprayed Al2o3, Al2o3-Tio2 Coatings
Authors: Šuopys Airingas , Marcinauskas Liutauras , Kėželis Romualdas , Aikas Mindaugas , Uscila Rolandas 
Published: March 2020

Abstract
In this article, the results of thermal and chemical resistance, of plasma sprayed Al2O3 and Al2O3-13wt.TiO2 coatings on a steel (P265GH) sample are presented. Coatings were formed using air-hydrogen plasma direct current plasma torch at atmospheric pressure. The resistance of coatings was measured by analyzing surface structure, elemental and phase composition of as sprayed coatings and after several heating cycles, imitating application conditions. To make this technology more appealing, by decreasing exploitation cost of standard cast iron grate used in straw pellet broilers, we used cheaper stainless steel with a protective Al2O3 and Al2O3-13wt.% TiO2 coatings. Most of the research done in this field is by using Ar as primary gas, but to decrease the manufacturing cost even further we used Air as primary gas. It was determined that after heat treatment the minor changes in the elemental composition were observed. Meanwhile an additional crystalline phases of metal oxides were detected.
Investigation on COVID-19 Infection Cases in Korea
Authors: Kang Yun Jung 
Published: March 2020

Abstract
On December 31st, 2019, the Chinese government announced officially that the country had a pneumonia case with an unknown cause. After that, Korea had 24 confirmed cases on February 8th, and the number has increased constantly since then. COVID-19, a highly contagious virus, infected another patient, Case No. 31, in Daegu; she was the first patient related to Sincheonji Church in Daegu. Later, the number of cases involved with Sincheonji skyrocketed. On March 6th, 2020, the accumulated number of confirmed cases was 6,284, with 42 dead among them. This study, through collecting epidemiological data about various COVID-19 infection cases, found out that getting together in large groups and religious ceremonies leads to massive infection, and that paying close attention to personal hygiene by wearing masks and sanitary gloves, etc., can prevent the spread of COVID-19. Additional epidemiological data and related studies on COVID-19 infections in Korea might either support or modify this conclusion. However, this study is significant in that it emphasizes the precautionary principle in preventing and managing infectious diseases, and that it has a suggestion for public health policies which are on urgent demand currently.
WITHDRAWN: Radiation-induced acute injury of intensity-modulated radiotherapy versus three-dimensional conformal radiotherapy in induction chemotherapy followed by concurrent chemoradiotherapy for nasopharyngeal carcinoma: a prospective cohort study
Authors: Yao Zexin , Lei Xiaoxuan , Chen Zhiyan , Pang Mengru , Huang Jialin , Cheng Biao 
Published: March 2020

Abstract
Background: Induction chemotherapy (IC) combined with concurrent chemoradiotherapy (CCRT) was the new standard treatment for nasopharyngeal carcinoma (NPC). We aimed to address whether the addition of intensity-modulated radiotherapy (IMRT) compared to three-dimensional conformal radiotherapy (3D-CRT) aggravate radiation-induced acute injury of these patients. Methods: We conducted a prospective study of consecutive patients with biopsy-proven nonmetastatic NPC who newly underwent radiotherapy and sequentially received IC, followed by CCRT at our institution. Among the 224 patients included, 108 (48.21%) were treated with IMRT and 116 (51.78%) with 3D-CRT. Clinicians evaluated routinely acute injury each day during radiotherapy according to the Radiation Therapy Oncology Group (RTOG). Radiation-induced dermatitis, oral mucositis, bone marrow toxicity and gastrointestinal toxicity were compared using the Kaplan-Meier method and the Log-rank test. Overall survival (OS), locoregional progression-free survival (LRFS) distant metastasis-free survival (DMFS) and progression-free survival (PFS) were analyzed during standard follow-up consultations. Results: There was no difference in patient characteristics between groups; however, IMRT plans had higher docetaxel and nedaplatin compared with 3D-CRT plans. The most severe acute toxicities included oral mucositis in 96.43% and dermatitis in 88.84%. Subset analysis revealed that Grade 3-4 acute dermatitis, oral mucositis and bone marrow toxicity were not significantly lower in the IMRT than 3D-CRT (P>0.05). Patients in IMRT group achieved significantly lower risk of bone marrow toxicity (Hazard ratio [HR]=2.557, P=0.0029) in Kaplan-Meier test, but higher risk of leukopenia (HR=0.492, P=0.0165) and gastrointestinal injury (HR=0.432, P=0.0011). Multivariate analyses also demonstrated that IMRT, male gender and hepatitis were the independent prognostic factor for bone marrow toxicity (HR=2.557, P=0.0032). No significant difference was found between the 3D-CRT and IMRT groups in terms of survival. The use of IMRT was a predictive factor of significantly better short-term efficacy, whereas anemia was a significant independent predictor. Conclusions: In a combined regimen of IC followed by CCRT for the treatment of NPC, IMRT seems to be an aggressive technique with a trend towards increased gastrointestinal and hematological toxicities than those treated with 3D-CRT. Predicting the occurrence time of various side effects is beneficial to early protection and focus on to coherent treatment.
Changes in the composition and function of the gut microbiome accompany type 1 diabetes in pregnancy
Authors: Roth-Schulze Alexandra J. , Penno Megan A. S. , Ngui Katrina M. , Oakey Helena , Thomson Rebecca L. , Vuillermin Peter J. , Craig Maria E , Rawlinson William D , Davis Elizabeth A , Harris Mark , Soldatos Georgia , Colman Peter G , Wentworth John M , Haynes Aveni , Morahan Grant , Bediaga Naiara G. , Smyth Gordon K , Papenfuss Anthony T. , Couper Jennifer J. , Harrison Leonard 
Published: March 2020

Abstract
Background The gut microbiome changes in response to a range of external influences, life events and disease. Pregnancy is a natural life event involving major physiological adaptation but studies of the microbiome across pregnancy are scarce and findings inconsistent. Pregnancy in which the mother has type 1 diabetes (T1D) with disturbed glucose-insulin homeostasis increases maternal and fetal risks and knowledge of the gut microbiome in this context is lacking. To understand how T1D impacts the gut microbiome in pregnancy we used a combination of whole metagenomic sequencing (WMS) and 16S rRNA sequencing to define the composition and function of the gut bacterial microbiome across 145 pregnancies, 94 in women with T1D. Women were participants in the Environmental Determinants of Islet Autoimmunity (ENDIA) study, in which the child has a first-degree relative with T1D.Results Pregnant women with and without T1D exhibited taxonomic and functional changes in gut microbial composition across pregnancy. Profiles in women with T1D were distinct, with a decrease and increase, respectively, of bacteria with anti- and pro-inflammatory properties, and a decrease in bacteria that synthesize essential vitamins and metabolites. These changes were accentuated in trimester 3, often in opposite directions to women without T1D.Conclusions The gut microbiome changes in composition and function across pregnancy, but distinctly in women with T1D. The decrease in bacteria that produce anti-inflammatory mediators and essential B-group vitamins suggests that the gut microbiome may contribute to maternal and fetal complications in the T1D pregnancy, potentially opening new avenues for therapeutic intervention.
Is correction of kyphosis necessary by posterior approach for the treatment of multilevel cervical degenerative myelopathy? An observational study
Authors: Du Wei , Wang Shuai , Wang Haixu , Zhang Jingtao , Wang Feng , Zhang Xu , Shen Yong 
Published: March 2020

Abstract
Background: The purpose of this study was to determine whether sagittal lordotic alignment, clinical outcomes and axial symptoms (AS) could be improved by kyphotic correction after posterior approach for multilevel cervical degenerative myelopathy (CDM).Methods: We retrospectively reviewed 109 patients with multilevel CDM with kyphosis who had undergone laminoplasty (Group LP, 53 patients) and laminectomy with lateral mass screw fixation (Group LCS, 56 patients) between January 2014 and December 2018. Curvature index (CI) was measured according to the pre- and postoperative radiographic parameters. The recovery rate was calculated based on the Japanese Orthopedic Association (JOA) score. AS severity was quantified by Neck Disability Index (NDI).Results: Analysis of postoperative follow-up data showed significant differences in CI (t = 8.64, P < 0.001), correction of CI (t = 8.97, P < 0.001) and NDI (t = 3.37, P < 0.001) between Group LP and LCS, whereas no significant differences in JOA score (t = 1.21, P = 0.23) and recovery rate (t = 1.52, P = 0.13). There were significant differences in JOA score (t = 98.29, 96.41, P < 0.001), CI (t = 17.07, 16.17, P < 0.001) and NDI (t = 37.46, 52.15, P < 0.001) between pre- and postoperative follow-up in Group LP and LCS. Correction of CI showed negative correlation with axial symptom severity (r = -0.51, P < 0.001), and no association with recovery rate (r = 0.14, P = 0.15).Conclusions: Satisfied neurological improvement was obtained by LP and LCS for patients with multilevel CDM, while kyphotic correction in Group LCS caused significant improvement of AS than that in Group LP. However, in a short-term postoperative follow-up, we could not demonstrate that kyphotic correction is associated with a better recovery in clinical outcomes.
Effects of Community-led Total Sanitation and Hygiene Implementation on Diarrheal Diseases Prevention in children less than five years of age in South Western Ethiopia: A Quasi- Experimental study
Authors: Bushen Gedamu , Merga Hailu , Tessema Fasil 
Published: March 2020

Abstract
Background: Diarrheal disease is one of the main causes of morbidity and mortality in children under five years of age in low and middle-income countries. Lack of improved sanitation is the most important contributing factor to diarrheal disease. Promotion of water, sanitation, and hygiene technologies combined with hygiene promotion is a key strategy for reducing diarrheal diseases in resource poor settings. Few studies have been conducted in rural areas of low-income countries where Community-Led Total Sanitation and Hygiene intervention is implemented. To our knowledge, no study was conducted in the study area to evaluate the effect of this intervention. This study was designed with the aim of finding the effects of Community-led Total Sanitation and Hygiene implementation for preventing diarrhea among under-five children.Methods: A community-based Quasi-Experimental study was conducted from March 01- April 10, 2019 in Kersa and Manna districts. Community-led Total Sanitation and Hygiene intervention is being implemented in Kersa district since 2005 Ethiopian calendar. Sample of 846 households were selected from intervention and comparison districts using four-stage random cluster-sampling method. Semi-structured questionnaire was used to collect data. Data was collected by 8 data collectors who attended secondary school. Data was cleaned, coded and entered into Epi data entry version 3.1 and exported to SPSS version 20 for analysis. Difference-in Difference method with McNemar’s tests was used to compare the prevalence of diarrhea between the intervention and comparison districts, and significance of change between the pre-test and post-test was declared at p-value of less than 0.05 with 95% confidence interval.Results: The intervention led to decreased diarrhea prevalence [pp = -8.2, 95% CI: -15.9, -0.5], increased latrine ownership [pp = 5.6, 95% CI: 0.5, 10.8], and increased latrine utilization [pp = 10.7, 95% CI: 4.7, 16.6] in intervention district at post-test compared to the baseline; while presence of hand washing facility near latrine, home based water treatment, and proper water storage and handling practice were decreased at post-test compared to the baseline.Conclusion: Implementation of Community-Led Total Sanitation improved sanitation and hygiene status of community that resulted in reduction of diarrhea prevalence in children less than five years of age. Further implementation, evaluation, and scale-up of the intervention is needed to reduce diarrheal disease in children less than five years of age.
Relationship of femoral offset and clinical outcomes of femoral neck fracture patients following bipolar hip arthroplasty
Authors: Kim Sung Soo , Kim Hyeon Jun , Lee Seung Yup 
Published: March 2020

Abstract
PurposeThis study aimed to investigate the relationship between femoral offset(FO) and clinical outcomes of patients with femoral neck fracture following bipolar hip arthroplasty(BHA). Materials and MethodsThis study recruited 520 patients who underwent BHA for femoral neck fracture from December 2003 to September 2018 with a minimum follow-up of one year. Excluding those with unclear medical records, a previous surgical history on the affected or contralateral hip, a congenital deformity and preoperative trauma history of the hip, high-energy multiple trauma, a history of neurodegenerative disease or cerebrovascular disease, a total of 77 patients were included in the analysis. The subjects were 54 females and 23 males. The mean age of patients was 74.6(65-95) years old and the mean follow-up period was 30.5 (12-136) months. For clinical assessment, postoperative pain visual analogue scale(VAS) and Harris hip score (HHS) were analyzed at each follow-up period. For radiological assessment, the difference in leg length discrepancy (LLD) and FO was measured. FO nearest to the real value was calculated using the values corrected for magnification and rotation errors on the anteroposterior view of plain radiographs taken at the first year of follow-up. In statistical analyses, the statistical significance between FO and clinical outcomes was analyzed using the Pearson correlation tests with SPSS v. 25.0. A value of p < 0.05 was considered statistically significant.ResultsThe difference in mean FO postoperatively was 6.7(±4.8) mm, and the difference in mean postoperative LLD was 4.9 (±3.4). The mean HHS was 77.1(±7.7), 82.3(±8.6), 83.4(±7.7), and 86.4(±6.7) at 1st , 3rd , 6th and 12th postoperative months, respectively. In the analysis using the Pearson correlation coefficient, the correlation coefficient between FO and HHS in 1-year follow-up was -0.38, and a significant outcome was found (p=0.001). Of HHS domain, the correlation coefficient for function in 1-year follow-up was -0.42, revealing a significant outcome (p=0.0001).ConclusionThere was a stastically significant correlation between clinical oucomes and FO difference at 1 year after BHA in patients with femoral neck fractures older than 65 years.
Proteins involved in the endoplasmic reticulum stress are key players in synovitis of osteoarthritis, chronic pyrophosphate arthropathy and rheumatoid arthritis, and correlate with the histological score
Authors: Seny Dominique de , Bianchi Elettra , Baiwir Dominique , Cobraiville Gaël , Collin Charlotte , Deliège Mégane , Kaiser Marie-Joëlle , Mazzucchelli Gabriel , Hauzeur Jean-Philippe , Delvenne Philippe , Malaise Michel 
Published: March 2020

Abstract
Background: It is now well recognized that osteoarthritis (OA) synovial membrane presents inflammatory components. The aim of this work is to provide evidence that similar inflammatory mechanisms exist among the three pathologies presenting an inflammatory gradient: OA, chronic pyrophosphate arthropathy (CPPA) and rheumatoid arthritis (RA).Methods: Synovial biopsies of OA (n=9), CPPA (n=7) and RA (n=8) patients were first characterized by a histological score based on synovial hyperplasia and infiltration of lymphocytes, plasma cells, polymorphonuclear and macrophages. All biopsies were also analyzed by 2D-nano-UPLC-ESI-Q-Orbitrap for protein identification and quantification. Protein levels were correlated with the histological score.Results: Histological score was in the range of 3 to 8 for OA, 5 to 13 for CPPA and 12 to 17 for RA. Of the 4336 proteins identified by mass spectrometry, 51 proteins were selected for their strong correlation (p<0.001) with the histological score of which 11 proteins (DNAJB11, CALR, ERP29, GANAB, HSP90B1, HSPA1A, HSPA5, HYOU1, LMAN1, PDIA4, and TXNDC5) were involved in the endoplasmic reticulum (ER) stress. Protein levels of S100A8 and S100A9 were significantly higher in RA compared to OA (for both) or to CPPA (for S100A8 only) and also significantly correlated with the histological score. Eighteen complement component proteins were identified, but only one (complement C1q subcomponent, subunit B and C) was weakly correlated with the histological score.Conclusions: This study highlights the inflammatory gradient existing between OA, CPPA and RA synovitis either at the protein level or at the histological level. Inflamed synovitis was characterized by the overexpression of ER stress proteins.
The challenge of multilateralism on the Ganges River
Authors: Williams Jessica M. 
Published: March 2020

AbstractThe Ganges River is traditionally governed bilaterally, with India at the centre of interactions. Bilateralism is arguable leveraged to India's advantage on a national and transboundary level. This is problematic as issues such as climate change require holistic and basin-wide solutions. Initiatives such as China's Belt and Road strategy are challenging Indian hegemony and pushing for multilateralism. The implications of this for transboundary water governance are investigated through discourse and the concept of discourse inertia. This shows how India is seeking to leverage its position in the sub-region through bilateralism and discursive tactics in response to China's increasing influence.
Distinguishing Enzymes and Non-enzymes Based on Structural Information with an Alignment Free Approach
Authors: Yang Lifeng , Jiao Xiong 
Published: March 2020

Background:
Knowledge of protein functions is very crucial for the understanding of biological processes. Experimental methods for protein function prediction are powerless to treat the growing amount of protein sequence and structure data.

Objective:
To develop some computational techniques for the protein function prediction.

Method:
Based on the residue interaction network features and the motion mode information, an
SVM model was constructed and used as the predictor. The role of these features was analyzed
and some interesting results were obtained.

Results:
An alignment-free method for the classification of enzyme and non-enzyme is developed in this work. There is not any single feature that occupies a dominant position in the prediction process. The topological and the information-theoretic residue interaction network features have a better performance. The combination of the fast mode and the slow mode can get a better explanation for the classification result.

Conclusion:
The method proposed in this paper can act as a classifier for the enzymes and nonenzymes.
Three Decades of Internet- and Computer-Based Interventions for the Treatment of Depression: Protocol for a Systematic Review and Meta-Analysis
Authors: Moshe Isaac , Terhorst Yannik , Cuijpers Pim , Cristea Ioana , Pulkki-Råback Laura , Sander Lasse 
Published: March 2020

Background
Depression is one of the leading causes of disability worldwide. Internet- and computer-based interventions (IBIs) have been shown to provide effective, scalable forms of treatment. More than 100 controlled trials and a growing number of meta-analyses published over the past 30 years have demonstrated the efficacy of IBIs in reducing symptoms in the short and long term. Despite the large body of research, no comprehensive review or meta-analysis has been conducted to date that evaluates how the effectiveness of IBIs has evolved over time.

Objective
This systematic review and meta-analysis aims to evaluate whether there has been a change in the effectiveness of IBIs on the treatment of depression over the past 30 years and to identify potential variables moderating the effect size.

Methods
A sensitive search strategy will be executed across the Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, EMBASE, and PsycINFO. Data extraction and evaluation will be conducted by two independent researchers. Risk of bias will be assessed. A multilevel meta-regression model will be used to analyze the data and estimate effect size.

Results
The search was completed in mid-2019. We expect the results to be submitted for publication in early 2020.

Conclusions
The year 2020 will mark 30 years since the first paper was published on the use of IBIs for the treatment of depression. Despite the large and rapidly growing body of research in the field, evaluations of effectiveness to date are missing the temporal dimension. This review will address that gap and provide valuable analysis of how the effectiveness of interventions has evolved over the past three decades; which participant-, intervention-, and study-related variables moderate changes in effectiveness; and where research in the field may benefit from increased focus.

Trial Registration
PROSPERO CRD42019136554; https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=136554

International Registered Report Identifier (IRRID)
PRR1-10.2196/14860