bluuebunny/crossref_metadata_2025_split huggingface dataset:
SHCBP1 interacting with EOGT enhanced O-GlcNAcylation of NOTCH1 and promoted the development of pancreatic cancer .
Authors: Yang Can , Hu Jianfei , Zhan Qian , Wang Zu-Wei , Li Ge , Pan Jing-Jing , Huang Long , Liao Cheng-Yu , Huang Yi , Tian Yi-Feng , Shen Bai-Yong , Chen Jiang-Zhi , Wang Yao-Dong , Chen Shi 
Published: October 2020

Abstract
Background: Pancreatic cancer(PDAC) remains one of the most lethal cancers worldwide, The accumulation of many abnormal epigenetic abnormalities contribute to the fatal prognosis of PDAC,While available studies are still limited. Many studies have confirmed that the Shc SH2-domain binding protein 1(SHCBP1) plays as proto-oncogene in cancers. However, whether SHCBP1 plays a role in pancreatic cancer oncogenesis is still unknown.EGF domain-specific O-linked GlcNActransferase (EOGT) acts as a key participant in the O-GlcNAcylation of NOTCH1. EOGT mutations inhibit the NOTCH1 signalling pathway and cause several congenital developmental disorders, However, the role of EOGT in malignancies has not been reported.Methods:SHCBP1 and EOGT were identified as proto-oncogenes in PDAC by High-throughput sequencing and Cox regression analysis,and validated by Internal and External cohort and public databases. The function of SHCBP1 and EOGT was determined in vitro and in vivo, The underlying mechanism was investigated by Western blot,Immunofluorescence（IF），Co-immunoprecipitation (Co-IP), Chromatin immunoprecipitation (ChIP)，and luciferase analyses.Results: The expression of EOGT and SHCBP1 was significantly elevated and correlated with worse prognosis in PDAC patients. In vitro, SHCBP1 overexpression promoted pancreatic cancer cell proliferation,migration and invasion, while knocking down SHCBP1 and EOGT inhibited these malignant processes. In vivo data showed that SHCBP1 overexpression promoted xenograft growth and lung metastasis and shortened survival in mice,whereas knocking down either EOGT or SHCBP1 expression suppressed xenograft growth and metastasis and prolonged survival. Morever，EOGT and SHCBP1 enhance the O-GlcNAcylation of NOTCH1, subsequently promoting the nuclear localization of the Notch intracellular domain (NICD) and inhibiting the transcription of E-cadherin and P21 in pancreatic cancer cells.Conclusion:All in all，This research revealed that SHCBP1 and EOGT act as proto-oncogenes in PDAC by enhancing the O-GlcNAcylation of NOTCH1，Which provides a new field of vision for new therapeutic targets for pancreatic cancer.
Let-7a suppresses Ewing sarcoma CSCs’ malignant phenotype via forming a positive feedback circuit with STAT3 and lin28
Authors: Xu Jiang , Zhang Zhongzu , Huang Lu , Zhou Rongping , Feng LinXuan , Zhou Zhenhai , Yu Honggui , Peng Kun , Li Chen , Liu Zhiming , Cao Kai 
Published: October 2020

Abstract
Background：Cancer stem cells (CSCs) have been documented to be closely related with tumor metastasis and recurrence, and the same important role were identified in Ewing Sarcoma (ES). In our previous study, we found that let-7a was repressed in ES. Herein, we further identified its putative effects in the CSCs of ES. Methods：Side population (SP) cells were isolated from ES cell lines A673 and SK-ES-1 via fluorescence-activated cell sorting (FACS) Assays and then identified. The expression of let-7a, signal Transducer and Activator of Transcription 3 (STAT3) and lin28 were detected by Real-time qPCR and western blotting. Cell colony formation assay and cell invasion assays were performed to detected the effect of let-7a on the malignant phenotype of ES SP cells. Finally, we injected the let-7a or scramble mimic into the xenograft tumors in nude mice to evaluated its effects on the growth of ES SP cells in vivo.Results：The expression of let-7a was consistently suppressed in the separated ES SP cells, which were identified to contain the characteristics of the stem cells. The ability of colony formation or invasion of ES SP cells was suppressed in vitro when we restored the expression of let-7a. The same results were found in the tumor growth of ES SP cells’ xenograft mice in vivo. Furthermore, we found STAT3 and lin28 were involved in the suppressive effects.Conclusion：Let-7a, STAT3 and lin28 might form a positive circuit to regulate the malignant phenotype of ES CSCs.
CDK5RAP3 as a Novel Biomarker Signature Predicting Survival and Adjuvant Chemotherapeutic Benefit in Gastric Cancer
Authors: Wang Jia-Bin , Gao You-Xin , Lian Ning-Zi , Ma Yu-Bin , Li Ping , Lin Jian-Xian , Lu Jun , Chen Qi-Yue , Cao Long-Long , Lin Mi , Liu Li-Chao , Tu Ru-Hong , Lin Ju-Li , Huang Ze-Ning , Zheng Hua-Long , Xie Jian-Wei , Zheng Chao-Hui , Huang Chang-Ming 
Published: October 2020

Abstract
BackgroundWe previously demonstrated that CDK5RAP3 acts as a tumour suppressor in gastric cancer through negative regulation of the Wnt/β-catenin signalling pathway, but its function in chemotherapeutic responsiveness of gastric cancer has not been investigated. MethodA collection of 188 pairs of tumour tissue microarray specimens from Fujian Medical University were employed for the discovery set, and 310 tumour tissue samples of gastric cancer patients were employed for the internal validation set. Eight-five tumour tissue samples from Qinghai University Hospital were used as the external validation set 1. Transcriptomic and clinical data of 299 gastric cancer patients from TCGA were used as the external validation set 2. CDK5RAP3 expression, microsatellite instability (MSI) status, and tumour-infiltrating lymphocytes (TIL) were examined with immunohistochemistry. Clinical outcomes of patients were compared with Kaplan-Meier curves and the Cox model.ResultsIn a multi-centre evaluation, increased CDK5RAP3 indication of better prognosis depends mainly on MSI-L/MSS status or TILhigh. High CDK5RAP3 expression predicts sensitive therapeutic responsiveness to postoperative adjuvant chemotherapy in gastric cancer. In a stratification analysis based on CDK5RAP3 combined with TIL or MSI status, patients with CKD5RAP3low TILlow showed no significant difference in prognosis after receiving chemotherapy, whereas patients with CKD5RAP3low TILhigh, CKD5RAP3high TILlow, and CKD5RAP3high TILhigh had better responsiveness to chemotherapy. In addition, patients with CKD5RAP3high MSI-L/MSS status benefitted the most from adjuvant chemotherapy among all patients evaluated. Conclusions﻿CKD5RAP3 can be used as an effective marker to evaluate individualized chemotherapy regimens in gastric cancer patients dependent on their TIL and MSI status.
Methodological Approaches to Imputing Early-Pregnancy Weight Based on Weight Measures Collected During Pregnancy
Authors: Yang Jiaxi , Wang Dongqing , Darling Anne Marie , Liu Enju , Perumal Nandita , Fawzi Wafaie W. , Wang Molin 
Published: October 2020

Abstract
Background: Early pregnancy weights are needed to quantify gestational weight gain accurately. Different methods have been used in previous studies to impute early-pregnancy weights. However, no studies have systematically compared imputed weight accuracy across different imputation techniques. This study aimed to compare four methodological approaches to imputing early-pregnancy weight, using repeated measures of pregnancy weights collected from two pregnancy cohorts in Tanzania.Methods: The mean gestational ages at enrollment were 17.8 weeks for Study I and 10.0 weeks for Study II. Given the gestational age distributions at enrollment, early-pregnancy weights were extrapolated for Study I and interpolated for Study II. The four imputation approaches included: (i) simple imputation based on the nearest measure, (ii) simple arithmetic imputation based on the nearest two measures, (iii) mixed-effects models, and (iv) generalized estimating equations. For the mixed-effects model and the generalized estimating equation model methods, imputation accuracy was further compared across varying degrees of model flexibility by fitting splines and polynomial terms. Additional analyses included dropping third-trimester weights, adding covariate to the models, and log-transforming weight before imputation. Mean absolute error was used to quantify imputation accuracy. Results: Study I included 1,472 women with 6,272 weight measures; Study II included 2,131 individuals with 11,775 weight measures. Among the four imputation approaches, mixed-effects models had the highest accuracy (smallest mean absolute error: 1.99 kg and 1.60 kg for Studies I and II, respectively), while the other three approaches showed similar degrees of accuracy. Depending on the underlying data structure, allowing appropriate degree of model flexibility and dropping remote pregnancy weight measures may further improve the imputation performance. Conclusions: Mixed-effects models had superior performance in imputing early-pregnancy weight compared to other commonly used strategies.
Sjogren's Syndrome in Clinical Trials of Traditional Chinese Medicine: Protocol for the Development of a Core Outcome Set
Authors: Chen Guangyan , Yuan Yuan , Liu Ming , Pu Bin 
Published: October 2020

Abstract
Background: Sjogren's syndrome (SS) is a chronic autoimmune rheumatic disease, and the incidence of 0.03% to 0.3%. There are an increasing number of randomised controlled trials of Traditional Chinese Medicine (TCM) for SS. However, there are generally some problems in clinical trials: lack of reporting primary or long term outcomes, and the heterogeneous in different clinical trials’ outcome. Our study aims to determine the priority outcomes and standard TCM syndromes for all stakeholders, and reach agreement on the core outcome set (COS) and syndromes to be measured and reported in all future TCM trials in patients with SS. Methods: A phase-wise refinement approach will be used, consisting of three phases, yet complementary, sub-work phases, whereby each phase will inform the next coming phases. The following are the three phases: (Ⅰ-a) Identifying of a long initial list of outcomes through: (1) systematic literature review; (2) semi-structured qualitative interviews. (Ⅰ-b) Identifying an initial list of TCM syndromes through: (1) systematic literature review; (2) referencing ancient Chinese medical books; (3) retrospective studies of medical records. (Ⅱ) Prioritization of outcomes using Delphi survey with different stakeholders, such as health professionals and patients. (Ⅲ) Through consensus meetings with patients and professionals to agree on the final COS and TCM syndromes.Results: We summarized the actions of COS into three points: direct action, indirect action, final action. Our study is very necessary.Conclusion: After the final COSs is completed, we will publish this research in a journal to promote communication.Trial registration number: Core Outcome Measures in Effectiveness Trials Initiative (COMET) number: 1429.
Impact of Pretreatment Anemia on Upfront Abiraterone Acetate Therapy for Metastatic Hormone-Sensitive Prostate Cancer: A Multicenter Retrospective Study
Authors: Okamoto Teppei , Noro Daisuke , Hatakeyama Shingo , Narita Shintaro , Sakurai Toshihiko , Mitsuzuka Koji , Kawamura Sadafumi , Hoshi Senji , Shimoda Jiro , Tanaka Toshikazu , Kawaguchi Toshiaki , Ishidoya Shigeto , Ito Akihito , Tsuchiya Norihiko , Habuchi Tomonori , Ohyama Chikara 
Published: October 2020

Abstract
Purpose: Anemia has been a known prognostic factor in metastatic hormone-sensitive prostate cancer (mHSPC). We therefore examined the effect of anemia on the efficacy of upfront abiraterone acetate (ABI) in patients with mHSPC.Methods: We retrospectively evaluated 67 mHSPC patients with high tumor burden who received upfront ABI between 2018 and 2020 (upfront ABI group). We divided these patients into two groups: the anemia-ABI group (hemoglobin <13.0g/dL, n =21) and the non-anemia-ABI group (n =46). The primary objective was to examine the impact of anemia on the progression-free survival (PFS; clinical progression including death from any cause) of patients in the upfront ABI group. Secondary objectives included an evaluation of the prognostic significance of upfront ABI and a comparison with a historical cohort (135 mHSPC patients with high tumor burden who received androgen deprivation therapy (ADT/complete androgen blockade [CAB] group) between 2014 and 2019).Results: We found that the anemia-ABI group had a significantly shorter PFS than the non-anemia-ABI group. A multivariate Cox regression analysis showed that anemia was an independent prognostic factor of PFS in the upfront ABI group (hazard ratio, 5.08; P =0.007). Patients in the non-anemia-ABI group were determined to have a significantly longer PFS than those in the non-anemia-ADT/CAB group (n =69) (P =0.002). However, no significant difference was observed in the PFS between patients in the anemia-ABI and the anemia-ADT/CAB groups (n =66). Multivariate analyses showed that upfront ABI could significantly prolong the PFS of patients without anemia (HR, 0.17; P = 0.003), whereas ABI did not prolong the PFS of patients with anemia.Conclusion: Pretreatment anemia was a prognostic factor among mHSPC patients who received upfront ABI. Although the upfront ABI significantly improved the PFS of mHSPC patients without anemia, its efficacy in patients with anemia might be limited.
Electron Generation in Water Induced by Magnetic Effect and Its Impact on Dissolved Oxygen Concentration
Authors: Yap Augustine Chung Wei , Lee Hwang Sheng , Loo Joo Ling , Mohd Nuruol Syuhadaa 
Published: October 2020

Abstract
pH, oxidation-reduction potential (ORP) and dissolved oxygen (DO) concentration are important water parameters in water quality surveillance and treatment. The changes of these water parameters are associated with electron density in water. Several techniques including electrolysis and catalysis which require redox reactions and electron exchange are employed to improve these water parameters. In recent years, studies reported that magnetic effects can impart considerable changes on the pH, ORP and DO concentration of water. However, the correlation between electron density and magnetic effects on these water parameters has yet to be disclosed despite the fact that increased electron density in water could improve water’s reductive properties, heat capacity and hydrogen bonding characteristics. In this study, the magnetic effects on water’s pH, ORP and DO concentration were investigated using different magnet arrangements and water flow rates based on reversed electric motor principle. Results showed that the improvement of pH, ORP and DO concentration by +3.51%, -6.89% and +5.62%, respectively were achieved using combined variables of non-reversed polarity magnet arrangement (1000-1500 Gauss magnetic strength) and water flow rate of 0.1-0.5 mL/s. Such decrement in ORP value also corresponded to 8.50 × 1013 number of electrons generation in water. Furthermore, Raman analysis revealed that magnetic effect could strengthen the intermolecular hydrogen bonding of water molecules and favour formation of smaller water clusters. The findings of this study could contribute to potential applications in aquaculture, water quality control and treatment of cancer attributed to free radical induced-oxidative stress.
Effect of Education on Knowledge, Attitude, and Performance of Elderly Caretakers in Nursing Homes of Mashhad City
Authors: Abbasi Fatemeh , Vizeshfar Fatemeh , Jahanbin Iran , Gafari Pyman 
Published: October 2020

Abstract
BackgroundThe population of the elderly taken care of at the nursing homes are increasing by the growth of the elderly’s population. Insufficient knowledge of the caretakers can affect the quality of their care from the elderly and result in poor performance. We aimed to evaluate the effect of education on knowledge, attitude, and performance of caretakers working at nursing homes.MethodsIn this quasi-experimental study, a total of 54 caretakers participated in Four-session classes and their knowledge, attitude, and performance towards the elderly were evaluated before, immediately after, and 1 month after the education by a researcher-designed questionnaire using SPSS software version 21.ResultsThe mean scores of the participants’ in attitude had no significant change knowledge scores at the three intervals, respectively (P=0.001) with a significant difference after the intervention compared with before (P<0.05). The performance scores at the three intervals, respectively (P<0.001) with a significant difference after the intervention compared with before (P<0.05). ConclusionThe significant improvement of knowledge and work performance of the elderly caretakers working at nursing homes shows the necessity of educating these individuals for better care from the elderly.
Dynamic Stall Control Over a Rotor Airfoil Based on AC DBD Plasma Actuation
Authors: Zhao Guangyin , Huang Yong , Yang Yongdong , Li Guoqiang , Yang Hesen 
Published: October 2020

Abstract
At present, the control capability of dielectric barrier discharge (DBD) plasma actuation covers the flow velocity range of helicopter’s retreating blades, so it is necessary to extend it to the dynamic stall control of rotor airfoils. A DBD plasma actuator was adopted to control the dynamic stall of an oscillating CRA309 airfoil in this paper. The effectiveness of alternating current (AC) DBD plasma actuation on reducing the area of lift hysteresis loop of the oscillating airfoil was verified through pressure measurements at a Reynolds number of 5.2×105. The influence of actuation parameters on the airfoil’s lift and moment coefficients was studied. Both steady and unsteady actuation could effectively reduce the hysteresis loop area of the lift coefficients. The flow control effect of dynamic stall was strongly dependent on the history of angle of attack. Compared with the steady actuation, unsteady actuation had more obvious advantages in dynamic stall control, with reducing the area of lift hysteresis loop by more than 30%. The effects of plasma actuation on the airfoil’s flow field at both upward and downward stages were discussed at last.
Contribution of Sarcomere Gene Mutations to Left Atrial Function in Patients With Hypertrophic Cardiomyopathy
Authors: Chung Hyemoon , Kim Yoonjung , Park Chul-Hwan , Kim In-Soo , Kim Jong-Youn , Min Pil-Ki , Yoon Young Won , Kim Tae Hoon , Lee Byoung Kwon , Hong Bum-Kee , Rim Se-Joong , Kwon Hyuck Moon , Lee Kyung-A , Choi Eui-Young 
Published: October 2020

Abstract
Background: Left atrial (LA) enlargement and dysfunction are related to clinical course in patients with hypertrophic cardiomyopathy (HCM). We aimed to investigate genetic contribution to LA structural and functional remodeling.Methods: 212 patients were consecutively enrolled, and echocardiography and extensive genetic analysis were performed. Cardiac magnetic resonance (CMR) was performed in 135 patients. Echocardiography was also performed in controls (n=30). Results: Patients with HCM had lower late-diastolic mitral annular velocity (a’) and higher LA volume index (LAVI) than controls. Patients with pathogenic or likely pathogenic sarcomere gene mutations (PSM, n = 67, 32%) had higher LAVI and lower CMR-derived LA total emptying fraction (37.0 ± 18.5 vs. 44.2 ± 12.4%, p=0.025). In patients without AF (n = 187), the PSM had lower a’ (6.9 ± 2.0 vs. 7.8 ± 1.9 cm/s, p = 0.004) than others. The PSM had higher prevalence and amount of late gadolinium enhancement (LGE) in the left ventricle (LV). In multivariate analysis, PSM was significantly related to lower a’ independent of E/e’, LV mass index, and LAVI. However, the relation significantly attenuated after adjustment for the extent of LGE in the LV, suggesting common myopathy in the LV and LA.Conclusions: PSM was related to LA dysfunction independent of LV filling pressure and LAVI, suggesting its contribution to atrial myopathy in HCM.
Gene Validation and Remodelling Using Proteogenomics of Phytophthora Cinnamomi, the Causal Agent of Dieback
Authors: Andronis Christina Eleni , Hane James K. , Bringans Scott , Hardy Giles E. S. , Jacques Silke , Lipscombe Richard , Tan Kar-Chun 
Published: October 2020

Abstract
Phytophthora cinnamomi is a pathogenic oomycete that causes plant dieback disease across a range of natural ecosystems and in many agriculturally important crops on a global scale. An annotated draft genome sequence and annotation is publicly available (JGI Mycocosm) and suggests 26,131 gene models. In this study, soluble mycelial, extracellular (secretome) and zoospore proteins of P. cinnamomi were exploited to refine the genome by correcting gene annotations and discovering novel genes. By implementing the diverse set of sub-proteomes into a generated proteogenomics pipeline, we were able to improve the P. cinnamomi genome. Liquid chromatography mass spectrometry was used to obtain high confidence peptides with spectral matching to both the annotated genome and a generated 6-frame translation. 2,764 annotations from the draft genome were confirmed by spectral matching. Using a proteogenomic pipeline, mass spectra were used to edit the P. cinnamomi genome and allowed identification of 23 new gene models and 60 edited gene features using high confidence peptides obtained by mass spectrometry, suggesting a rate of incorrect annotations of 3% of the detectable proteome. The novel features were further validated by total peptide support, alongside functional analysis including the use of Gene Ontology and functional domain identification. We demonstrated the use of spectral data in combination with our proteogenomics pipeline can be used to improve the genome of important plant diseases and identify biologically relevant missed genes. This study presents the first use of spectral data to edit and manually annotate an oomycete pathogen.
Seasonal Breeding Leads to Changes for Gut Microbiota Diversity in the Wild Ground Squirrel (Spermophilus Dauricus)
Authors: Yang Xiaoying , Yao Yuchen , Zhang Xueying , Zhong Jiahui , Gao Fuli , Zhang Haolin , Han Yingying , Weng Qiang , Yuan Zhengrong 
Published: October 2020

Abstract
Background: Seasonal breeding is a normal phenomenon that animals adapt to natural selection and reproduce only in specific seasons. With the gradual popularization of Next-generation sequencing (NGS), large studies have shown that seasonal breeding has been affected by gut microbiota. Consequently, the purpose of this study is to explore the effect of seasonal breeding on the gut microbiota of wild ground squirrel (Spermophilus dauricus). We used 16S rRNA gene sequencing technology to sequence the gut microbiota of the wild ground squirrel in the breeding season and non-breeding season. We also predicted the function of gut microbiota by bioinformatic software.Results: The results showed that the main components of gut microbiota in all samples consisted of Firmicutes (61.8%), Bacteroidetes (32.4%), and Proteobacteria (3.7%). Microbial community composition analyses revealed significant differences between these two groups. At the genus level, Alistipes, Mycoplasma, Anaerotruncus, and Odoribacter were up-regulated in the non-breeding season, while Blautia and Streptococcus spp. were up-regulated in the breeding season. The result of function prediction suggested that the relative abundance of functional categories related to lipid metabolism, carbohydrate metabolism, and nucleotide metabolism was higher in the breeding season. The expression of transcription, energy metabolism, and signal transduction was enriched in the non-breeding season. Conclusions: Overall, the results of this study emphasized the significant effects of seasonal breeding on gut microbiota community composition of the wild ground squirrel and laid a foundation for further study of gut microbiota on seasonal breeding in the future.