bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating the Effects of a Brief Tobacco Intervention in the US Air Force
Authors: Little Melissa A , Fahey Margaret C , Klesges Robert C , McMurry Timothy , Talcott Gerald W 
Published: January 2020

Abstract

Introduction
Military personnel have among the highest rates of tobacco use in the United States. Unfortunately, there are few interventions aimed at reducing tobacco use among this vulnerable population. The current study addresses this need by evaluating the short-term effectiveness of a Brief Tobacco Intervention (BTI), a 40-min group-based intervention designed to reduce contemporary patterns of tobacco use among a sample of US military enlistees during an 11-week period of involuntary tobacco abstinence.

Aims and Methods
Participants were 2999 US Air Force Technical Trainees at Joint Base San Antonio-Lackland Air Force Base in San Antonio, Texas from April 2017 through January 2018. Participants were cluster randomized to three conditions: (1) BTI + Airman’s Guide to Remaining Tobacco Free (AG), (2) AG intervention, or (3) standard smoking cessation intervention. The primary analysis was a comparison of the interventions’ efficacies in preventing tobacco use during Technical Training, conducted using a generalized estimating equations logistic regression model controlling for covariates. Multiple imputation was used to account for loss to follow-up.

Results
There was not a significant difference by condition in the use of tobacco products at follow-up (p = .454). The BTI + AG condition did produce short-term changes in perceived harm, intentions to use tobacco, knowledge about tobacco products, and normative beliefs.

Conclusions
These findings suggest that while the intervention was effective in the short term, it was not potent enough over a 12-week period to prevent Airmen from initiating tobacco use. Future studies should examine whether adding a booster session or media campaign enhances the effectiveness of the intervention.

Implications
Despite the fact that most Airmen believe they will remain tobacco free following the ban in Technical Training, a large percentage of these Airmen resume and initiate tobacco use during this high-risk period. As a result, there is a need for interventions targeting the range of tobacco available to military trainees during a teachable moment when they report intentions to remain tobacco free. The current study shows that a BTI has promise in reducing long-term tobacco use, when coupled with additional interventions, such as a booster session or a media campaign.
Petrologic Insights into Rift Zone Magmatic Interactions from the 2011 Eruption of Kīlauea Volcano, Hawaiʻi
Authors: Walker Brett H , Garcia Michael O , Orr Tim R 
Published: January 2020

AbstractThe high frequency of historical eruptions at Kīlauea Volcano presents an exceptional opportunity to address fundamental questions related to the transport, storage, and interaction of magmas within rift zones. The Nāpau Crater area on Kīlauea’s East Rift Zone (ERZ) experienced nine fissure eruptions within 50 years (1961–2011). Most of the magma intruded during these frequent eruptions remained stored within the rift zone, creating a potential magma mixing depot within the ERZ. The superbly monitored and sampled 2011 eruption (Puʻu ʻŌʻō episode 59) presents an extraordinary opportunity to evaluate magma mixing processes within the ERZ. Whole-rock, glass, and olivine compositions were determined, not only for lava from the 2011 eruption, but also for a new suite of Nāpau Crater area samples from the 1963, 1965, 1968, 1983, and 1997 eruptions, as well as the previously undocumented 1922 eruption. Whole-rock XRF data revealed two geochemically distinct magma batches for episode 59: one less evolved (∼6·6 wt % MgO, 0·46 wt % K2O) than the other (∼6·2 wt % MgO, 0·58 wt % K2O). Episode 59 lava is remarkably aphyric (∼0·1 vol. % phenocrysts), making use of mineralogy to identify parent magma affinities problematic. Linear compositional trends of whole-rock major and trace elements, and reversely zoned olivine crystals indicate episode 59 lavas underwent magma mixing. Least squares regression calculations and plots of major and trace element data, were used to evaluate whether the episode 59 samples are products of mixing summit-derived magma with residual magma from previous Nāpau Crater area eruptions. The regression results and trace element ratios are inconsistent with previously proposed mixing scenarios, but they do support mixing between summit-derived magma and residual magma from the 1983 and 1997 Nāpau Crater area eruptions. These magmas were stored in physically and chemically distinct pods at depths of 1·6–3·0 km prior to mixing with new magma intruded from the summit to produce the episode 59 lava. One pod contained a fractionated equivalent of 1983 lava, and the other a hybrid of compositions similar to 1983 and 1997 lavas. The petrology of episode 59 lava demonstrates that magmas from two previous eruptions (1983 and 1997) were available to mix with magma intruded from the summit region. This study clarifies the pre-eruptive history of the mixed episode 59 lava, and elucidates the evolution of the volcano's magmatic system in a region of frequent eruptions.
Molecular phylogeny of freshwater snails and limpets (Panpulmonata: Hygrophila)
Authors: Saadi Ahmed J , Davison Angus , Wade Christopher M 
Published: January 2020

AbstractWe have undertaken a comprehensive molecular phylogenetic analysis of the Hygrophila based on 3112 sites of the large subunit and 5.8S ribosomal RNA genes. A clear basal division between Chilinoidea and Lymnaeoidea is observed. The monophyly of Acroloxidae, Lymnaeidae and Physidae is also well established. However, Planorbidae are not supported as a monophyletic group, because the Bulinidae cluster within Planorbidae. The Amphipepleinae within Lymnaeidae and both Planorbinae and Ancylinae within Planorbidae are strongly supported as monophyletic subfamilies. However, the Aplexinae within Physidae and the Lymnaeinae within Lymnaeidae are not recovered. A new taxonomic revision of the Hygrophila is proposed based on the findings of this molecular phylogeny, and the implications for the evolution of chirality are discussed.
A Simple Intervention to Decrease Nutrient Losses in Continuous Feeds With Human Milk
Authors: Davidson Jennifer , Elabiad Mohamad T. 
Published: January 2020

ABSTRACTObjectives:Current evidence suggests that a significant amount of human milk fat is lost because of tubing adsorption. The objective was to evaluate the effect of reintroducing tubing leftover milk on the quality of macronutrient content of delivered milk.Methods:This was an in vitro study. The standard method of preparing and delivering fortified human milk includes preparing a small extra volume that would be used for priming the connection tubes. At the end of the infusion, the tubes and any milk leftover would be discarded. This method was compared with a new method where by the exact milk volume was prepared and used to prime the connection tubes. Leftover tubing milk was pushed with air.Results:The standard method was associated with significant losses in fat, protein, and calories of 16.7%, 3.4%, and 9.2% compared with the new method of 8.2%, 0%, and 3.3%, respectively. These losses in the standard method were predominantly explained by the significant gains in the left over milk contents of fat 6.3 ± 1.1 g/dL, protein 3.5 ± 0.4 g/dL, and calories 28 ± 2.6 kcal/oz as compared with prepared milk of 4.8 ± 0.3, 2.9 ± 0.3 g/dL, and 24.0 ± 0.8 kcal/oz respectively, P = 0.002.Conclusions:Traditional continuous delivery methods of human milk are associated with significant losses of fat and protein. By preparing the exact amount of ordered milk and then pushing through the residual milk left in the tubing with a small amount of air, this study offers a simple intervention that would significantly decrease these losses.
Altered Gut Microbiota Is Present in Newly Diagnosed Pediatric Patients With Inflammatory Bowel Disease
Authors: Sila Sara , Jelić Marko , Trivić Ivana , Tambić Andrašević Arjana , Hojsak Iva , Kolaček Sanja 
Published: January 2020

ABSTRACTBackground and aims:Clinical and experimental data suggest that gut microbiota plays an important role in the pathogenesis of inflammatory bowel disease (IBD). The aim of this study was to determine intestinal microbiota in newly diagnosed patients with IBD and to compare it with patients’ healthy siblings who share same genetic and environmental background and to healthy unrelated controls.Methods:Molecular approach targeting 16S ribosomal RNA was employed for analyzing the gut microbiota of participants’ stool samples. Terminal restriction fragment length polymorphphism analysis was performed.Results:Newly diagnosed pediatric patients with IBD (n = 19, 68.4% Crohn disease [CD], mean age 14.8 ± 0.65 years), their unaffected healthy siblings (n = 20, mean age 12.8 ± 0.85 years), and unrelated healthy controls (n = 19, mean age 10.7 ± 0.8 years) were included. Microbial diversity differed significantly between IBD patients, healthy siblings, and healthy controls (P = 0.018 for MspI digestion, P = 0.013 for HhaI digestion). No significant difference in microbial diversity was found between healthy siblings and healthy controls. In patients reduced presence of genus Eubacterium, Lactobacillus, Enterobacter and Clostridium, and increased presence of genus Streptococcus, Prevotella and Escherichia, compared with healthy siblings and healthy controls, was found.Conclusion:Newly diagnosed pediatric patients with IBD show significantly less diverse microbiota and microbial composition compared with healthy siblings and healthy controls.
Reliability of Visual Analog Scale and Numeric Rating Scale for the Assessment of Disease Activity in Systemic Lupus Erythematosus
Authors: Elera-Fitzcarrald Claudia , Vega Karen , Gamboa-Cárdenas Rocío V. , Zúñiga Katiuska , Zevallos Francisco , Reátegui-Sokolova Cristina , Pastor-Asurza César , Perich-Campos Risto , Rodríguez Bellido Zoila , Aranow Cynthia , Alarcón Graciela S. , Calvo Armando , Ugarte-Gil Manuel F. 
Published: January 2020

Objective
To determine the reliability of SLE patients' disease activity measurements.

Methods
This was a cross-sectional study conducted (August 2016–December 2017) at 2 main public Peruvian hospitals, 1 with a comprehensive lupus care program. Patients assessed their disease activity with a visual analog scale (VAS) (0–100 mm) or a numerical rating scale (NRS) (0–4) before and after their physician's (MD's) assessment. Demographic and disease-related characteristics were recorded. Reliability of patients' disease activity before and after MD's assessment was determined using Spearman rank correlation. Factors possibly associated with this variability were examined with Spearman rank correlation and Mann-Whitney U test.

Results
Two hundred forty, mostly Mestizo, SLE patients were included; mean (SD) age and disease duration (diagnosis) were 34.9 (12.9) years and 10.1 (7.0) years, respectively. The Mexican version of the Systemic Lupus Erythematosus Disease Activity Index was 1.9 (2.7), and the Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index was 1.2 (1.5). The correlations between NRS and VAS before and after the MD's assessment were ρ = 0.839; p < 0.001; and ρ = 0.872; p < 0.001, respectively. Visual analog scale and NRS were higher before than after the MD's assessment (VAS 29.3 [26.5] and 26.5 [24.9], p = 0.052; and NRS (1.5 [1.2] and 1.3 [1.1], p = 0.003); only the comprehensive program explained this variability (p = 0.043). The reliability of VAS and NRS was ρ = 0.917 and ρ = 0.861, p < 0.001, before and after for the comprehensive program and ρ = 0.710 and ρ = 0.785, p < 0.001, for before and after for the regular program.

Conclusions
Both VAS an NRS are highly reliable. Patients scored higher before than after their physicians' assessment but that these differences were smaller for the patients in the comprehensive care program than in the regular one.
Novel FARS2 variants in patients with early onset encephalopathy with or without epilepsy associated with long survival
Authors: Barcia Giulia , Rio Marlène , Assouline Zahra , Zangarelli Coralie , Roux Charles-Joris , de Lonlay Pascale , Steffann Julie , Desguerre Isabelle , Munnich Arnold , Bonnefont Jean-Paul , Boddaert Nathalie , Rötig Agnès , Metodiev Metodi D. , Ruzzenente Benedetta 
Published: January 2020

AbstractMitochondrial translation is essential for the biogenesis of the mitochondrial oxidative phosphorylation system (OXPHOS) that synthesizes the bulk of ATP for the cell. Hypomorphic and loss-of-function variants in either mitochondrial DNA or in nuclear genes that encode mitochondrial translation factors can result in impaired OXPHOS biogenesis and mitochondrial diseases with variable clinical presentations.Compound heterozygous or homozygous missense and frameshift variants in the FARS2 gene, that encodes the mitochondrial phenylalanyl-tRNA synthetase, are commonly linked to either early-onset epileptic mitochondrial encephalopathy or spastic paraplegia. Here, we expand the genetic spectrum of FARS2-linked disease with three patients carrying novel compound heterozygous variants in the FARS2 gene and presenting with spastic tetraparesis, axial hypotonia and myoclonic epilepsy in two cases.
Surgical candidacy and treatment uptake among women with cervical cancer at public referral hospitals in Kampala, Uganda
Authors: Swanson Megan L , Nakalembe Miriam , Chen Lee-may , Ueda Stefanie M , Namugga Jane , Nakisige Carol , Huchko Megan J 
Published: January 2020

AbstractPurposeCervical cancer is the most common malignancy among women in Uganda. Most present with advanced disease, when hysterectomy is not possible and cure is less likely. This study reports the proportion recommended for hysterectomy and associated factors, recommended treatments by stage, and treatment uptake.MethodsWe conducted a prospective study among patients seeking care for cervical cancer at public referral hospitals in Uganda. In-person surveys were followed by a phone call. Descriptive and multivariate statistical analyses examined associations between predictors and outcomes.ResultsAmong 268 participants, 76% were diagnosed at an advanced stage (IIB-IVB). In total, 12% were recommended for hysterectomy. In adjusted analysis, living within 15 kilometers of Kampala (OR 3.10, 95% CI 1.20-8.03) and prior screening (OR 2.89, 95% CI 1.22-6.83) were significantly associated with surgical candidacy. Radiotherapy availability was not significantly associated with treatment recommendations for early-stage (IA-IIA) disease, but was associated with recommended treatment modality (chemo-radiation versus primary chemotherapy) for locally advanced stage (IIB-IIIB). Most (67%) had started treatment. No demographic or health factor, treatment recommendation, or radiation availability was associated with treatment initiation. Among those recommended for hysterectomy, 55% underwent surgery. Among those who had initiated treatment, 82% started the modality that was actually recommended.ConclusionWomen presented to public referral centers in Kampala with mostly advanced-stage cervical cancer and few were recommended for surgery. Lack of access to radiation did not significantly increase the proportion of early-stage cancers recommended for hysterectomy.
Non-canonical glutamate signaling in a genetic model of migraine with aura
Authors: Parker Patrick D. , Suryavanshi Pratyush , Melone Marcello , Reinhart Katelyn M. , Sawant-Pokam Punam M. , Kaufmann Dan , Theriot Jeremy J. , Pugliese Arianna , Conti Fiorenzo , Shuttleworth C. William , Pietrobon Daniela , Brennan K.C. 
Published: January 2020

SummaryMigraine with aura is an extremely common but poorly understood sensory circuit disorder. Monogenic models allow an opportunity to understand its mechanisms, in particular because the migraine aura is associated with spreading depolarizations that can be measured physiologically. Using fluorescent glutamate imaging in awake mice carrying a familial hemiplegic migraine type 2 mutation, we recorded previously undescribed spontaneous ‘plumes’ of glutamate signaling that anatomically overlapped with reduced density of GLT-1a positive astrocyte processes. These events could be mimicked in wild-type animals by inhibition of glutamate clearance, which we show to be slower during sensory processing in FHM2 carriers. Plumes depended on calcium mediated vesicular release from neurons, but not action potentials. Importantly, a rise in both basal glutamate and plume frequency predicted the onset of spreading depolarization in WT and FHM2 animals, providing a novel mechanism in migraine with aura and by extension the many other neurological disorders where spreading depolarizations occur.
VolPy: automated and scalable analysis pipelines for voltage imaging datasets
Authors: Cai Changjia , Friedrich Johannes , Pnevmatikakis Eftychios A , Podgorski Kaspar , Giovannucci Andrea 
Published: January 2020

AbstractVoltage imaging enables monitoring neural activity at sub-millisecond and sub-compartment scale, and therefore opens the path to studying sub-threshold activity, synchrony, and network dynamics with unprecedented spatio-temporal resolution. However, high data rates (>800MB/s) and low signal-to-noise ratios have created a severe bottleneck for analysis of such datasets. Here we presentVolPy, the first turn-key, automated and scalable pipeline to pre-process voltage imaging datasets.VolPyfeatures fast motion correction, memory mapping, segmentation, and spike inference, all built on a highly parallelized and computationally efficient framework that optimizes memory and speed. Given the lack of single cell voltage imaging ground truth examples, we introduce a corpus of 24 manually annotated datasets from different preparations and voltage indicators. We benchmarkVolPyagainst this corpus and electrophysiology recordings, demonstrating excellent performance in neuron localization, spike extraction, and scalability.
AMPKα1 is essential for Glucocorticoid Receptor triggered anti-inflammatory macrophage activation
Authors: Caratti Giorgio , Desgeorges Thibaut , Juban Gaëtan , Koenen Mascha , Kozak Bozhena , Théret Marine , Chazaud Bénédicte , Tuckermann Jan P , Mounier Rémi 
Published: January 2020

SummaryMacrophages are key immune cells which mediate both the acute inflammatory phase and the repair phase after tissue damage. Macrophages switch from pro-inflammatory to anti-inflammatory cells that sustain repair and return to tissue homeostasis. We show that the metabolic sensor, AMP-activated protein kinase (AMPK) is essential for glucocorticoid induction of an anti-inflammatory macrophage phenotype. While canonical gene regulation by glucocorticoids was not affected by loss of AMPK, we identified AMPK-dependent glucocorticoid-regulated genes in macrophages, related to efferocytosis. AMPK-deficient macrophages do not acquire phenotypic and functional anti-inflammatory features upon glucocorticoid exposure. We identified FOXO3 as an AMPK-dependent regulator of glucocorticoid activity in macrophages. Loss of AMPK in macrophagesin vivoabrogates glucocorticoid anti-inflammatory actions during post-injury muscle regeneration and endotoxin induced acute lung injury. These data highlight that the glucocorticoid receptor is dependent on AMPK for its immunomodulatory actions in macrophages, linking their metabolic status to transcriptional control in resolving inflammation.
Near-chromosome level genome assembly of the fruit pestDrosophila suzukiiusing long-read sequencing
Authors: Paris Mathilde , Boyer Roxane , Jaenichen Rita , Wolf Jochen , Karageorgi Marianthi , Green Jack , Cagnon Mathilde , Parinello Hugues , Estoup Arnaud , Gautier Mathieu , Gompel Nicolas , Prud’homme Benjamin 
Published: January 2020

ABSTRACTOver the past decade, the spotted wing Drosophila, Drosophila suzukii, has invaded Europe and America and has become a major agricultural pest in these areas, thereby prompting intense research activities to better understand its biology. Two draft genome assemblies based on short-read sequencing were released in 2013 for this species. Although valuable, these resources contain pervasive assembly errors and are highly fragmented, two features limiting their values. Our purpose here was to improve the assembly of theD. suzukiigenome. For this, we generated PacBio long-read sequencing data at 160X sequence coverage and assembled a novel, contiguousD. suzukiigenome. We obtained a high-quality assembly of 270 Mb (with 546 contigs, a N50 of 2.6Mb, a L50 of 15, and a BUSCO score of 95%) that we called WT3-2.0. We found that despite 16 rounds of full-sib crossings theD. suzukiistrain that we sequenced has maintained high levels of polymorphism in some regions of its genome (ca. 19Mb). As a consequence, the quality of the assembly of these regions was reduced. We explored possible origins of this high residual diversity, including the presence of structural variants and a possible heterogeneous admixture pattern of North American and Asian ancestry. Overall, our WT3-2.0 assembly provides a higher quality genomic resource compared to the previous one in terms of general assembly statistics, sequence quality and gene annotation. This newD. suzukiigenome assembly is therefore an improved resource for high-throughput sequencing approaches, as well as manipulative genetic technologies to studyD. suzukii.
Human Genes AreIn SilicoPotential Targets For Rice miRNA
Authors: Rakhmetullina Aizhan , Pyrkova Anna , Aisina Dana , Ivashchenko Anatoliy 
Published: January 2020

AbstractExogenous miRNAs enter the human body through food, and their effects on metabolic processes can be considerable. It is important to determine which miRNAs from plants affect the expression of human genes and the extent of their influence. The binding sites of 738 osa-miRNAs that interact with 17508 mRNAs of human genes were determined using the MirTarget program. The characteristics of the binding of 46 single osa-miRNAs to 86 mRNAs of human genes with a value of free energy (ΔG) interaction equal 94% to 100% from maximum ΔG were established. The findings showed that osa-miR2102-5p, osa-miR5075-3p, osa-miR2097-5p, osa-miR2919 targeted the largest number of genes at 38, 36, 23, 19 sites, respectively. mRNAs of 86 human genes were identified as targets for 93 osa-miRNAs of all family osa-miRNAs with ΔG values equal 94% to 98% from maximum ΔG. Each miRNA of the osa-miR156-5p, osa-miR164-5p, osa-miR168-5p, osa-miR395-3p, osa-miR396-3p, osa-miR396-5p, osa-miR444-3p, osa-miR529-3p, osa-miR1846-3p, osa-miR2907-3p families had binding sites in mRNAs of several human target genes. The binding sites of osa-miRNAs in mRNAs of the target genes for each family of osa-miRNAs were conserved when compared to flanking nucleotide sequences. mRNA human genes of osa-miRNAs are candidate genes of cancer, cardiovascular and neurodegenerative diseases.