bluuebunny/crossref_metadata_2025_split huggingface dataset:
Positive and negative cooperativity of TNF and Interferon-γ in regulating synovial fibroblast function and B cell survival in fibroblast/B cell co-cultures
Authors: Lowin Torsten , Anssar Tareq M. , Bäuml Marina , Classen Tim , Schneider Matthias , Pongratz Georg 
Published: January 2020

AbstractSynovial fibroblasts (SF) were reported to produce B cell activating factor (BAFF) in response to stimulation with interferon-γ (IFN-γ) or tumor necrosis factor (TNF). However, the influence of these pro-inflammatory cytokines on other receptors/ligands of the TNF superfamily or associated cytokine receptors in SF has not been investigated yet. Here we show the differential regulation of BAFF (CD257), Fn14 (CD266), TACI (CD267), BAFF-R (CD268), BCMA (CD269), CD40 ligand (CD40L, CD154), IFN-γR (CD119), Leptin receptor (ObR, CD295), VCAM-1 (CD106) and membrane TGF-β in isolated SF and the impact of IFN-γ/TNF co-incubation on proliferation, IL-6 and IL-8 production. In addition, the impact of differentially stimulated SF on B cell survival in co-cultures was assessed. Surface cytokines and cytokine receptors were detected by flow cytometry. Soluble cytokine receptors and cytokines were quantified by ELISA. Proliferation was assessed by cell titer blue. Murine B cell survival in fibroblast/ B cell co-cultures was determined by annexin V/propidium iodide staining and flow cytometry. IFN-γ together with TNF synergistically and significantly increased the cell surface levels of BAFF, Fn14, TACI, BAFF-R, BCMA, CD40L, ObR and IFN-γR in rheumatoid arthritis SF after 72 h incubation. Soluble BAFF was only induced by IFN-γ and inhibited by TNF. Addition of TWEAK had no influence on proliferation or IL-8 production but decreased TNF-induced IL-6 production, whereas APRIL, BAFF and leptin did not modulate TNF or TNF/IFN-γ-induced proliferation or cytokine production. Proliferation was increased by TNF and further enhanced by the addition of IFN-γ. In co-culture experiments, SF stimulated with TNF/IFN but not TNF or IFN-γ alone increased shedding of VCAM-1 and expression of membrane TGFβ, which was associated with reduced survival of murine B cells. IFN-γ and TNF regulate the expression of TNF family member cytokines and associated receptors. Ligation of IFN-γR and Fn14 under pro-inflammatory conditions modulated IL-6/IL-8 production and proliferation. In B cell/SF co-cultures, the combination of TNF/IFN reduced B cell survival possibly via enhanced VCAM-1 shedding and/or increased TGF-β production. IFN-γ is necessary for the observed effects on B cell survival and SF cytokine production and emphasizes its anti-inflammatory role in rheumatoid arthritis.
The flexible linker of the secreted FliK ruler is required for export switching of the flagellar protein export apparatus
Authors: Kinoshita Miki , Tanaka Seina , Inoue Yumi , Namba Keiichi , Aizawa Shin-Ichi , Minamino Tohru 
Published: January 2020

AbstractThe hook length of the flagellum is controlled to about 55 nm in Salmonella. The flagellar type III protein export apparatus secretes FliK to determine hook length during hook assembly and changes its substrate specificity from the hook protein to the filament protein when the hook length has reached about 55 nm. Salmonella FliK consists of an N-terminal domain (FliKN, residues 1–207), a C-terminal domain (FliKC, residues 268–405) and a flexible linker (FliKL, residues 208–267) connecting these two domains. FliKN is a ruler to measure hook length. FliKC binds to a transmembrane export gate protein FlhB to undergo the export switching. FliKL not only acts as part of the ruler but also contributes to this switching event, but it remains unknown how. Here we report that FliKL is required for efficient interaction of FliKC with FlhB. Deletions in FliKL not only shortened hook length according to the size of deletions but also caused a loose length control. Deletion of residues 206–265 significantly reduced the binding affinity of FliKC for FlhB, thereby producing much longer hooks. We propose that an appropriate length of FliKL is required for efficient interaction of FliKC with FlhB.
Xanthine oxidase inhibition attenuates insulin resistance and diet-induced steatohepatitis in mice
Authors: Nishikawa Tomoki , Nagata Naoto , Shimakami Tetsuro , Shirakura Takashi , Matsui Chieko , Ni Yinhua , Zhuge Fen , Xu Liang , Chen Guanliang , Nagashimada Mayumi , Yamashita Taro , Sakai Yoshio , Yamashita Tatsuya , Mizukoshi Eishiro , Honda Masao , Kaneko Shuichi , Ota Tsuguhito 
Published: January 2020

AbstractHyperuricemia drives the development of nonalcoholic fatty liver disease (NAFLD). Pharmacological inhibition of xanthine oxidase (XO), a rate-limiting enzyme for uric acid (UA) production, has been demonstrated to improve hepatic steatosis in diet-induced obese mice. However, it remains unclear whether inhibition of XO improves nonalcoholic steatohepatitis (NASH), a more advanced form of NAFLD, in terms of both liver inflammation and fibrosis. Here, we investigated the effects of febuxostat and allopurinol, two XO inhibitors clinically used for gout, on a mouse model of NASH. Furthermore, we conducted a single-arm, open-label intervention study with febuxostat for NAFLD patients with hyperuricemia. Despite a similar hypouricemic effect of the XO inhibitors on blood UA level, febuxostat, but not allopurinol, significantly decreased hepatic XO activity and UA levels in the NASH model mice. These reductions in hepatic XO activity and UA levels were accompanied by attenuation of insulin resistance, lipid peroxidation, and classically activated M1-like macrophage accumulation in the liver. Furthermore, in NAFLD patients with hyperuricemia, treatment with febuxostat for 24 weeks decreased the serum UA level, accompanied by reductions in the serum levels of liver enzymes, alanine aminotransferase and aspartate aminotransferase. XO may represent a promising therapeutic target in NAFLD/NASH, especially in patients with hyperuricemia.
Response of the Salmon Heart Transcriptome to Pancreas Disease: Differences Between High- and Low-Ranking Families for Resistance
Authors: Robinson N. A. , Krasnov A. , Burgerhout E. , Johnsen H. , Moghadam H. K. , Hillestad B. , Aslam M. L. , Baranski M. , Boison S. A. 
Published: January 2020

AbstractPancreas disease caused by salmonid alphaviruses leads to severe losses in Atlantic salmon aquaculture. The aim of our study was to gain a better understanding of the biological differences between salmon with high and low genomic breeding values (H-gEBV and L-gEBV respectively) for pancreas disease resistance. Fish from H- and L-gEBV families were challenged by intraperitoneal injection of salmonid alphavirus or co-habitation with infected fish. Mortality was higher with co-habitation than injection, and for L- than H-gEBV. Heart for RNA-seq and histopathology was collected before challenge and at four- and ten-weeks post-challenge. Heart damage was less severe in injection-challenged H- than L-gEBV fish at week 4. Viral load was lower in H- than L-gEBV salmon after co-habitant challenge. Gene expression differences between H- and L-gEBV manifested before challenge, peaked at week 4, and moderated by week 10. At week 4, H-gEBV salmon showed lower expression of innate antiviral defence genes, stimulation of B- and T-cell immune function, and weaker stress responses. Retarded resolution of the disease explains the higher expression of immune genes in L-gEBV at week 10. Results suggest earlier mobilization of acquired immunity better protects H-gEBV salmon by accelerating clearance of the virus and resolution of the disease.
Enhancement of Colonic Absorptive Function after the Massive Resection of the Small Intestine Based on the Creation of an Artificial Colonic Valve
Authors: Deng Gaoyan , Deng Zhijian 
Published: January 2020

AbstractThe colon can have an absorptive function similar to that of the small intestine after the massive resection of the small bowel. To improve colonic absorptive function, we created a valve in the colon (artificial colonic valve, ACV). ACVs were created in 20 rats that had 80 percent of their small intestine resected, with an observation time of 30 weeks. The ACV rats were compared with those in the non-operated control group, the short bowel syndrome (SBS) group and the colon interposition (CI) group. The ACV rats were much heavier than those in the control group, SBS group and CI group. In terms of histology and the levels of α-amylase and the Na+-dependent bile salt transporter, the absorptive function of the colons before the valves resembled that of the small intestine. The colonic absorptive function was more obvious in ACV rats than in CI rats. An ACV can enhance colonic absorptive function after the massive resection of the small intestine. The colonic absorptive function of ACV rats was better than that of the rats in the CI group.
Diagnostic value of non-contrast brain computed tomography in the evaluation of acute cerebral venous thrombosis
Authors: Tayyebi Shamim , Akhavan Reza , Shams Majid , Salehi Maryam , Farrokh Donya , Yousefi Farhad , Abbasi Bita 
Published: January 2020

AbstractAcute cerebral vein thrombosis is usually seen as increased attenuation in brain non-contrast computed tomography. It is so helpful to define measurable parameters for subjective evaluation of sinus thrombosis in non-enhanced brain computed tomography, especially where advanced neuroimaging techniques are not available. The purpose of this study is to evaluate the diagnostic value of venobasilar attenuation ratio and venobasilar attenuation difference in the evaluation of acute cerebral venous sinuous thrombosis in non-enhanced brain CT scan. Fifty confirmed cases of acute cerebral vein thrombosis were sex- and age-matched with 73 subjects who did not have the condition. Average venous sinus attenuation, Hounsfield to hematocrit ratio, basilar artery density, venobasilar attenuation ratio and venobasilar attenuation difference were measured. Mean attenuation was 65.8 in thrombosed and 44.9 in non-thrombosed sinuses (P < 0.0001). A cutoff absolute sinus attentuaion of 61 HU led to a sensitivity of 82%, specificity of 100% and accuracy of 92%. A cutoff ratio of 1.4 for venobasilar ratio led to a sensitivity of 100%, specificity of 78% and accuracy of 87%. A cut-off value of 24 for venobasilar difference resulted in the sensitivity of 80%, specificity of 100% and accuracy of 92%. The additional measurement of venous sinus and basilar artery attenuations and calculation of venobasilar ratio and difference can increase the sensitivity and specificity of NCCT in the diagnosis of acute CVST.
Integrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal
Authors: Casserly Alison P. , Tsuji Junko , Zhao-Shea Rubing , Smith Ciearra B. , Molas Susanna , Tapper Andrew R. , Weng Zhiping , Gardner Paul D. 
Published: January 2020

AbstractTobacco use is the leading preventable cause of mortality in the world. The limited number of smoking cessation aids currently available are minimally effective, highlighting the need for novel therapeutic interventions. We describe a genome-wide approach to identify potential candidates for such interventions. Next-generation sequencing was performed using RNA isolated from the habenulo-interpeduncular circuit of male mice withdrawn from chronic nicotine treatment. This circuit plays a central role in the nicotine withdrawal response. Differentially expressed miRNAs and mRNAs were validated using RT-qPCR. Many of the differentially expressed mRNAs are predicted targets of reciprocally expressed miRNAs. We illustrate the utility of the dataset by demonstrating that knockdown in the interpeduncular nucleus of a differentially expressed mRNA, that encoding profilin 2, is sufficient to induce anxiety-related behavior. Importantly, profilin 2 knockdown in the ventral tegmental area did not affect anxiety behavior. Our data reveal wide-spread changes in gene expression within the habenulo-interpeduncular circuit during nicotine withdrawal. This dataset should prove to be a valuable resource leading to the identification of substrates for the design of innovative smoking cessation aids.
Effect of UVA1 on hypertrophic scarring in the rabbit ear model
Authors: Zhang Tong , Shen Zhiming , Zheng Jie , Jiang Rui 
Published: January 2020

AbstractHypertrophic scars (HTSs) are common and cause functional and psychological morbidity. UVA1 (340–400 nm) phototherapy has been previously shown to be effective in the treatment of localized scleroderma, systemic sclerosis, and POEMS syndrome with minimal side effects, all of which are presented as collagen fibrils hyperplasia that is common with scarring in skin histology. In the present study, we aimed to investigate the impact of UVA1 on the protein expression of TGF-β signal pathway and myofibroblasts in a rabbit model of cutaneous scarring. Full-thickness skin wounds (2 cm × 5 cm in diameter) were made in New Zealand white rabbits to establish the hypertrophic scarring model. New Zealand white rabbits were divided into two treatment groups (n=30 wounds per group with an equal number of controls): medium-dose of UVA1 phototherapy group: 60 J/cm2; high-dose of UVA1 phototherapy group: 110 J/cm2. Left ears were used for treatment and the right ones were used for control. Treatment was administered five times weekly for 6 weeks. Treated and untreated control wounds were harvested at various time points and examined by histologic examination, immunohistochemical assessment, and ultrastructural evaluation. The results showed that UVA1 phototherapy caused a significant reduction in dermal thickness by histological features, whereas the scar index was descended significantly in both medium- and high-dose UVA1 groups compared with the control group. Examination of immunohistochemistry also revealed a marked suppression of tissue growth factor-β (TGF-β) (both medium- and high-dose), α smooth muscle actin (α-SMA) (only high-dose), and tissue inhibitor of metalloproteinase 1 (TIMP-1) (only high-dose), and apparent increase in matrix metalloproteinases (MMP-1) (both medium- and high-dose) compared with the control. The ultrastructural evaluation showed the collagen fibers’ diameter had shrunk, and that fibroblastic cytoplasm was not affluent and in a quiescent stage. These findings of the present study suggested that administration of UVA1 irradiation is effective to improve the experimental HTS model and raises a possibility of the therapeutic approach of UVA1 in the scar. Although not directly examined in the present study, MMP inhibition is hypothesized to be responsible for this effect. However, early UVA1 treatment could not prevent the formation of scar model.
Astragalus polysaccharide attenuates metabolic memory-triggered ER stress and apoptosis via regulation of miR-204/SIRT1 axis in retinal pigment epithelial cells
Authors: Peng Qing-Hua , Tong Ping , Gu Li-Min , Li Wen-Jie 
Published: January 2020

Abstract
Background: ‘Metabolic memory’ of early hyperglycaemic environment has been frequently suggested in the progression of diabetic retinopathy (DR). Retinal pigment epithelial (RPE) cells are crucial targets for DR initiation following hyperglycaemia. Astragalus polysaccharides (APS) has been long used as a traditional Chinese medicine in treating diabetes. In the present study, the preventive effects and mechanisms of APS on metabolic memory-induced RPE cell death were investigated.
Methods: The expressions of miR-204 and SIRT1 were determined by reverse transcription quantitative PCR (RT-qPCR). Dual luciferase assay was applied to detect the potential targeting effects of miR-204 on SIRT1. SIRT1, ER stress and apoptosis related proteins were monitored using Western blotting. Apoptosis was assessed by TUNEL assay and Annexin V/PI staining followed by flow cytometry analysis. MiR-204 mimics and shSIRT1 were applied for miR-204 overexpression and SIRT1 knockdown, respectively.
Results: High glucose exposure induced metabolic memory, which was accompanied with sustained dysregulation of miR-204/SIRT1 axis, high level of ER stress and activation of apoptotic pathway even after replacement with normal glucose. Pre-treatment with APS concentration-dependently reversed miR-204 expression, leading to disinhibition of SIRT1 and alleviation of ER stress-induced apoptosis indicated by decreased levels of p-PERK, p-IRE-1, cleaved-ATF6, Bax, cleaved caspase-12, -9, -3, and increased levels of Bcl-2 and unleaved PARP. The effects of APS on RPE cells were reversed by either miR-204 overexpression or SIRT1 knockdown.
Conclusions: We concluded that APS inhibited ER stress and subsequent apoptosis via regulating miR-204/SIRT1 axis in metabolic memory model of RPE cells.
The H IX galaxy survey
Authors: Lutz K. A. , Kilborn V. , Catinella B. , Cortese L. , Brown T. H. , Koribalski B. 
Published: January 2020

Context. This paper presents the analysis of optical integral field spectra for the H I eXtreme (H IX) galaxy sample. H IX galaxies host at least 2.5 times more atomic gas (H I) than expected from their optical R-band luminosity. Previous examination of their star formation activity and H I kinematics suggested that these galaxies stabilise their large H I discs (radii up to 94 kpc) against star formation due to their higher than average baryonic specific angular momentum. A comparison to semi-analytic models further showed that the elevated baryonic specific angular momentum is inherited from the high spin of the dark matter host.
Aims. In this paper we now turn to the gas-phase metallicity as well as stellar and ionised gas kinematics in H IX galaxies to gain insights into recent accretion of metal-poor gas or recent mergers.
Methods. We compare the stellar, ionised, and atomic gas kinematics, and examine the variation in the gas-phase metallicity throughout the stellar disc of H IX galaxies.
Results. We find no indication for counter-rotation in any of the components, the central metallicities tend to be lower than average, but as low as expected for galaxies of similar H I mass. Metallicity gradients are comparable to other less H I-rich, local star forming galaxies.
Conclusions. We conclude that H IX galaxies show no conclusive evidence for recent major accretion or merger events. Their overall lower metallicities are likely due to being hosted by high spin halos, which slows down their evolution and thus the enrichment of their interstellar medium.
A broadband X-ray view of the NLSy1 1E 0754.6+3928
Authors: Middei R. , Tombesi F. , Vagnetti F. , Serafinelli R. , Bianchi S. , Miniutti G. , Marinucci A. , Matzeu G. A. , Petrucci P.-O. , Ursini F. , Zaino A. 
Published: January 2020

Context. The soft X-ray band of many active galactic nuclei (AGNs) is affected by obscuration due to partially ionised matter crossing our line of sight. In this context, two past XMM-Newton observations that were six months apart and a simultaneous NuSTAR-Swift exposure of the Narrow Line Seyfert 1 galaxy 1E 0754.6+3928, which was approximately eight years later, revealed an intense and variable warm absorber (WA) and hints of additional absorbers in the Fe Kα band.
Aims. We aim to provide the first X-ray characterisation of this AGN by discussing its broadband (0.3–79 keV) spectrum and temporal properties.
Methods. We conduct a temporal and spectroscopic analysis on two ∼10 ks (net exposure) XMM-Newton snapshots performed in April and October 2006. We also study the high energy behaviour of 1E 0754.6+3928 and model its broadband spectrum by using simultaneous Swift-NuSTAR data. Both phenomenological and physically motivated models are tested.
Results. We find the presence of flux variability of ∼150% and 30% for 0.3–2 and 2–10 keV bands, respectively, and spectral changes at months timescales of ΔΓ ∼ 0.4. A reflection component that is consistent with being constant over years and arising from relatively cold material that is far from the central super massive black hole is detected. The main spectral feature shaping the 1E 0754.6+3928 spectrum is a WA. Such a component is persistent over the years and the variability of its ionisation and column density is observed down on months in the ranges 3 × 1022cm−2 ≲  NH ≲ 7.2 × 1022cm−2 and 1.5 ≲ log (ξ/erg s−1 cm) ≲ 2.1. Despite the short exposures, we find possible evidence of two additional high-ionisation and high-velocity outflow components in absorption.
Conclusions. Our analysis suggests the existence of a complex system of absorbers in 1E 0754.6+3928. Longer exposures are required in order to characterise, on more solid grounds, the absorbers in this AGN.
Longitudinal filament oscillations enhanced by two C-class flares
Authors: Zhang Q. M. , Guo J. H. , Tam K. V. , Xu A. A. 
Published: January 2020

Context. Large-amplitude, longitudinal filament oscillations triggered by solar flares have been well established in the literature. However, filament oscillations enhanced by flares have never been reported.
Aims. In this paper we report the multiwavelength observations of a very long filament in active region (AR) 11112 on 2010 October 18. The filament was composed of two parts, the eastern part (EP) and the western part (WP). We focus on longitudinal oscillations of the EP, which were enhanced by two homologous C-class flares in the same AR.
Methods. The filament was observed in Hα wavelength by the Global Oscillation Network Group and in extreme ultraviolet wavelengths by the Atmospheric Imaging Assembly on board the Solar Dynamics Observatory (SDO). Line-of-sight magnetograms were provided by the Helioseismic and Magnetic Imager on board SDO. The global three-dimensional magnetic fields were obtained using the potential field source surface modeling. Soft X-ray light curves of the two flares were recorded by the GOES spacecraft. White light images of the corona were observed by the LASCO/C2 coronagraph on board SOHO. To reproduce part of the observations, we perform one-dimensional, hydrodynamic numerical simulations using the MPI-AMRVAC code.
Results. The C1.3 flare was confined without a coronal mass ejection (CME). Both EP and WP of the filament were slightly disturbed and survived the flare. After 5 h, eruption of the WP generated a C2.6 flare and a narrow jet-like CME. Three oscillating threads (thda, thdb, thdc) are obviously identified in the EP, and their oscillations are naturally divided into three phases by the two flares. The initial amplitude ranges from 1.6 to 30 Mm with a mean value of ∼14 Mm. The period ranges from 34 to 73 min with a mean value of ∼53 min. The curvature radii of the magnetic dips are estimated to be 29 to 133 Mm with a mean value of ∼74 Mm. The damping times ranges from ∼62 to ∼96 min with a mean value of ∼82 min. The value of τ/P is between 1.2 and 1.8. For thda in the EP, the amplitudes were enhanced by the two flares from 6.1 Mm to 6.8 Mm after the C1.3 flare, and further to 21.4 Mm after the C2.6 flare. The period variation as a result of perturbation from the flares was within 20%. The attenuation became faster after the C2.6 flare.
Conclusions. To the best of our knowledge, this is the first report of large-amplitude, longitudinal filament oscillations enhanced by flares. Numerical simulations reproduce the oscillations of thda very well. The simulated amplitudes and periods are close to the observed values, while the damping time in the last phase is longer, implying additional mechanisms should be taken into account apart from radiative loss.