bluuebunny/crossref_metadata_2025_split huggingface dataset:
Managing incursions of Vespa velutina nigrithorax in the UK: an emerging threat to apiculture
Authors: Jones Eleanor P. , Conyers Chris , Tomkies Victoria , Semmence Nigel , Fouracre David , Wakefield Maureen , Stainton Kirsty 
Published: November 2020

AbstractVespa velutina nigrithorax is an invasive species of hornet accidentally introduced into Europe in 2004. It feeds on invertebrates, including honey bees, and represents a threat to European apiculture. In 2016, the first nest of this hornet was detected and destroyed on mainland UK. A further 8 nests were discovered between 2016 and 2019. Nest dissection was performed on all nests together with microsatellite analyses of different life stages found in the nests to address the reproductive output and success of nests found in the UK. None of the nests had produced the next generation of queens. Follow-up monitoring in those regions detected no new nests in the following years. Diploid males were found in many UK nests, while microsatellite analysis showed that nests had low genetic diversity and the majority of queens had mated with one or two males. All UK nests derived from the European zone of secondary colonisation, rather than from the native range of the species. None of the nests discovered so far have been direct offspring of another UK nest. The evidence suggests that these nests were separate incursions from a continental population rather than belonging to a single established UK population of this pest.
Recycling biofloc waste as novel protein source for crayfish with special reference to crayfish nutritional standards and growth trajectory
Authors: Lunda Roman , Roy Koushik , Dvorak Petr , Kouba Antonin , Mraz Jan 
Published: November 2020

AbstractScreening of novel feedstuffs, that too for data-deficient (nutritionally) animals, is somewhat ambiguous or problematic. Through systematic meta-analyses, the present study formulated most up-to-date crayfish nutritional standards, against which a recyclable waste (biofloc biomass, BM) from intensive aquaculture systems was assessed as a novel protein source. Growth trajectory dependencies and thermal growth coefficient qualifying for good growth in crayfish (TGC 0.5–0.64 units) were benchmarked. Using these standards and a 7-week growth trial, BM's suitability as a novel protein source for red swamp crayfish Procambarus clarkii was evaluated through its graded inclusions in a commercial feed. Results suggest that BM can elevate growth at 33–66% inclusion in existing feed formulations. Beyond 66% inclusion, BM can deteriorate growth in crayfish due to high ash content (exceeding physiological limit > 14%), arginine deficiency (~ 14–20% lower than an optimum requirement), and insufficient non-protein energy: protein ratio (3.7 cal mg−1). Arginine is perhaps the most critical amino acid in dietary protein for crayfish, and deficient in BM. Although no critical bioaccumulation levels of heavy metals were breached by feeding 100% BM to crayfish, a mineral and heavy metal (Hg) stress seemed plausible. Crayfish raised solely on biofloc may not realize full growth potential.
Pattern formation in reaction–diffusion system on membrane with mechanochemical feedback
Authors: Tamemoto Naoki , Noguchi Hiroshi 
Published: November 2020

AbstractShapes of biological membranes are dynamically regulated in living cells. Although membrane shape deformation by proteins at thermal equilibrium has been extensively studied, nonequilibrium dynamics have been much less explored. Recently, chemical reaction propagation has been experimentally observed in plasma membranes. Thus, it is important to understand how the reaction–diffusion dynamics are modified on deformable curved membranes. Here, we investigated nonequilibrium pattern formation on vesicles induced by mechanochemical feedback between membrane deformation and chemical reactions, using dynamically triangulated membrane simulations combined with the Brusselator model. We found that membrane deformation changes stable patterns relative to those that occur on a non-deformable curved surface, as determined by linear stability analysis. We further found that budding and multi-spindle shapes are induced by Turing patterns, and we also observed the transition from oscillation patterns to stable spot patterns. Our results demonstrate the importance of mechanochemical feedback in pattern formation on deforming membranes.
HRRD: a manually-curated database about the regulatory relationship between HPV and host RNA
Authors: Yan Bingqing , Zhang Siwei , Yu Siyang , Hussain Sajjad , Liu Tianyang , Wang Bozhi , Dong Xiaoyu , Ma Fen , Wei Lanlan 
Published: November 2020

AbstractHPV (Human papilloma virus) is a kind of small double-stranded DNA viruses which is extremely associated with different cancers. The roles HPV plays in the host were gradually identified through the interaction between it (including its early genes) and host RNA. In recent years, increasing numbers of studies in HPV-related cancers have been published showing the relationship between HPV and host RNA. Here, we present a database named HRRD, which contains the regulatory relationship between HPV and RNA (mRNA, miRNA and lncRNA). The information was extracted from 10,761 papers in PubMed (up to December 1st, 2019). In addition, the sequence map of HPV (198 genotypes) is also contained. HRRD was designed as a user-friendly web-based interface for data retrieval. It integrated the information of interaction between HPV and RNA, which reflects the relationship between HPV and host. We hope HRRD will further provide a comprehensive understanding of HPV in carcinogenesis and prognosis. HRRD is freely accessible at www.hmuhrrd.com/HRRD.
Validation of red blood cell flux and velocity estimations based on optical coherence tomography intensity fluctuations
Authors: Marchand Paul J. , Lu Xuecong , Zhang Cong , Lesage Frédéric 
Published: November 2020

AbstractWe present a validation of red blood cell flux and speed measurements based on the passage of erythrocytes through the OCT’s focal volume. We compare the performance of the so-called RBC-passage OCT technique to co-localized and simultaneously acquired two-photon excitation fluorescence microscopy (TPEF) measurements. Using concurrent multi-modal imaging, we show that fluctuations in the OCT signal display highly similar features to TPEF time traces. Furthermore, we demonstrate an overall difference in RBC flux and speed of 2.5 ± 3.27 RBC/s and 0.12 ± 0.67 mm/s (mean ± S.D.), compared to TPEF. The analysis also revealed that the OCT RBC flux estimation is most accurate between 20 RBC/s to 60 RBC/s, and is severely underestimated at fluxes beyond 80 RBC/s. Lastly, our analysis shows that the RBC speed estimations increase in accuracy as the speed decreases, reaching a difference of 0.16 ± 0.25 mm/s within the 0–0.5 mm/s speed range.
Control over the fibrillization yield by varying the oligomeric nucleation propensities of self-assembling peptides
Authors: Lau Chun Yin Jerry , Fontana Federico , Mandemaker Laurens D. B. , Wezendonk Dennie , Vermeer Benjamin , Bonvin Alexandre M. J. J. , de Vries Renko , Zhang Heyang , Remaut Katrien , van den Dikkenberg Joep , Medeiros-Silva João , Hassan Alia , Perrone Barbara , Kuemmerle Rainer , Gelain Fabrizio , Hennink Wim E. , Weingarth Markus , Mastrobattista Enrico 
Published: November 2020

AbstractSelf-assembling peptides are an exemplary class of supramolecular biomaterials of broad biomedical utility. Mechanistic studies on the peptide self-assembly demonstrated the importance of the oligomeric intermediates towards the properties of the supramolecular biomaterials being formed. In this study, we demonstrate how the overall yield of the supramolecular assemblies are moderated through subtle molecular changes in the peptide monomers. This strategy is exemplified with a set of surfactant-like peptides (SLPs) with different β-sheet propensities and charged residues flanking the aggregation domains. By integrating different techniques, we show that these molecular changes can alter both the nucleation propensity of the oligomeric intermediates and the thermodynamic stability of the fibril structures. We demonstrate that the amount of assembled nanofibers are critically defined by the oligomeric nucleation propensities. Our findings offer guidance on designing self-assembling peptides for different biomedical applications, as well as insights into the role of protein gatekeeper sequences in preventing amyloidosis.
Thermal decomposition of hybrid ultramicroporous materials (HUMs)
Authors: Healy Colm , Harvey-Reid Nathan C. , Howard Ben I. , Kruger Paul E. 
Published: November 2020

The thermal decomposition behaviour of representative hybrid ultramicroporous materials (HUMs) is investigated. Decomposition is triggered by fragmentation of the inorganic pillar, yielding XF4 gases and metal fluorides.
Lactobacillus rhamnosus GG soluble mediators ameliorate early life stress-induced visceral hypersensitivity and changes in spinal cord gene expression
Authors: McVey Neufeld Karen-Anne , Strain Conall R. , Pusceddu Matteo M. , Waworuntu Rosaline V. , Manurung Sarmauli , Gross Gabriele , M. Moloney Gerry , Hoban Alan E. , Murphy Kiera , Stanton Catherine , Dinan Timothy G. , Cryan John F. , O'Mahony Siobhain M. 
Published: November 2020

Abstract
Visceral hypersensitivity is a hallmark of many functional and stress-related gastrointestinal disorders, and there is growing evidence that the gut microbiota may play a role in its pathophysiology. It has previously been shown that early life stress-induced visceral sensitivity is reduced by various probiotic strains of bacteria (including Lactobacillus rhamnosus GG (LGG)) alone or in combination with prebiotic fibres in rat models. However, the exact mechanisms underpinning such effects remain unresolved. Here, we investigated if soluble mediators derived from LGG can mimic the bacteria’s effects on visceral hypersensitivity and the microbiota–gut–brain axis. Rats were exposed to maternal separation (MS) from postnatal days 2–12. From weaning onwards both non-separated (NS) and MS offspring were provided drinking water with or without supplementation of standardized preparations of the LGG soluble mediators (LSM). Our results show that MS led to increased visceral sensitivity and exaggerated corticosterone plasma levels following restraint stress in adulthood, and both of these effects were ameliorated through LSM supplementation. Differential regulation of various genes in the spinal cord of MS versus NS rats was observed, 41 of which were reversed by LSM supplementation. At the microbiota composition level MS led to changes in beta diversity and abundance of specific bacteria including parabacteroides, which were ameliorated by LSM. These findings support probiotic soluble mediators as potential interventions in the reduction of symptoms of visceral hypersensitivity.
Study on 3D printing technology and mechanical properties of a nano‐enhanced composite hydrogel bio‐ink
Authors: Wu Weitao 
Published: November 2020

Traditional bio‐inks are mostly limited in application due to their low‐mechanical strength or complex cross‐linking curing methods. In this study, a nanocomposite hydrogel bio‐ink [gelatin–alginate–montmorillonite (GT–AT–MMT)] for 3D bioprinting is designed based on the thermo‐sensitive properties of GT, the advantages of ionic crosslinking of sodium AT, and the shear thinning and toughening mechanism of nano‐MMT. The 3D printing of the bio‐ink with variable AT content is optimised, and the tensile, compressive, and creep properties of the 3D printed structure are studied. Results show that GT–AT–MMT bio‐ink has good printability, and its mechanical properties are enhanced with increased AT content. The tensile modulus of the GT–4% AT–MMT sample can reach 0.533 MPa, which has a minimum creep deformation of 0.311 mm under the same load condition. Therefore, GT–AT–MMT bio‐ink provides a new possibility for the development of 3D bioprinting, which will offer many opportunities in fields such as tissue engineering, drug delivery, and regeneration medicine.
Facile approach to synthesise zeolite X and its adsorption for Cu (II) and Zn(II)
Authors: Shi Bin , Chang Qing 
Published: November 2020

Pure zeolite X was synthesised by a facile microwave‐assisted two‐step approach using high silicon fly ash, industrial‐grade reagents and tap water. First, a microwave heating method was conducted to extract silicon and the extract process was completed in 20 min, which was more effective. Then, a microwave approach was used to synthesis zeolite X. The whole analysis process is <2 h. The composition, phase structure, morphology, particle size distribution, specific surface areas and adsorption of heavy metal ions were evaluated comprehensively. The results show that single phase zeolite X with octahedral structure and an average diameter of 1.10 μm is obtained, and the Brunauer–Emmett–Teller surface area is 583.22 m2/g. Especially, the adsorption of Cu2+ and Zn2+ are as high as 81.33 and 67.21 mg/g, respectively. It can be concluded that the Letter provides a facile and fast method utilising high silicon fly ash to synthetise zeolite X, which is an excellent adsorbent to remove heavy metal ions.
Peony‐like Na2Mg(CO3)2: a nanomaterial with the characteristic of high sensitivity and rapid response for the detection of alcohols
Authors: Cai Xinli , Liu Fei , Sun Bai , Xu Fangwen , Tang Zhuo , Zhang Jie , Cheng Yunming , Liu Jinyun , Zhu Shuguang 
Published: November 2020

Alcohol has a potential health risk to human health, so it is important to realise the rapid detection of alcohols. Based on this, the nano Na2Mg(CO3)2 was prepared by a simple hydrothermal method to establish a cataluminescence (CTL) gas sensor for alcohols. The Na2Mg(CO3)2with highly purified quality was characterised by scanning electron microscope, energy dispersive spectroscopy and the powder X‐ray diffraction. The results show that the nano Na2Mg(CO3)2 is a peony‐like substance with a large surface area, which will be beneficial to produce ultra‐weak chemiluminescence reaction between the detector and the material. The peony‐like Na2Mg(CO3)2 has good CTL response to alcohols (methanol, ethanol, n‐propanol and n‐butanol), showing high selectivity. The response time of Na2Mg(CO3)2 for four alcohols is between 1 and 2 s, which indicate that the material has the advantage of rapid response. The limit of detection of four alcohols defined as 3 N/S is about 7.91, 4.75, 6.69 and 8.10 mg/l, respectively. The optimum reaction conditions of the gas sensor based on nano Na2Mg(CO3)2 are mild and this nanomaterial can be used for alcohol detection.
Multilevel non‐volatile memory based on Al2O3/ZnO bilayer device
Authors: Arya Lekshmi Jagath , Nandha Kumar Thulasiraman , Jinesh Kochupurackal 
Published: November 2020

This Letter reports the multilevel resistive switching characteristics of bipolar bi‐layer nanodevice made of Ag/Al2O3(10 nm)/ZnO (5 nm)/fluorine‐doped tin oxide for the first time, exhibiting five distinguishable resistive states including both high resistance state (HRS) and low resistance state (LRS). Among five distinct memory states, three resistive states are attained with RESET voltages and two are attained with SET voltages. The distinguishable resistive levels obtained with RESET voltages 2.3, 3.0 and 3.8 V are 1300, 2700 and 4320 Ω, respectively, whereas two stable resistive levels attained with SET voltages 0.8 and 1.70 V are 3000 and 100 Ω, respectively. Moreover, the proposed device excels in its Roff/Ron ratio of 354 obtained with HRS/LRS at −1.4 V as well as the ratio of intermediate states IRS1, IRS2, IRS3 to HRS becomes 15, 04 and 27, respectively. Further, the conduction mechanism as well as the switching mechanism are demonstrated. Finally, the cycle‐to‐cycle as well as device‐to‐device variability in device characteristics and statistical distribution of their resistance levels are also analysed and report that devices in the sample are also exhibiting stable multilevel capability.
Syndrome de Brodie sévère : prise en charge orthodontique et chirurgicale
Authors: Rousselet Clothilde , Frans Charles , Bouckenooghe Hadrien , Guillaumot Geneviève , Racy Emmanuel , Le Norcy Elvire 
Published: November 2020

Ce rapport décrit le traitement orthodontico-chirurgical d’une patiente présentant une exocclusion unilatérale sévère avec égression dentaire majeure associée à une biproalvéolie. La patiente âgée de 27 ans et demi s’est présentée afin de poursuivre un traitement orthodontique démarré un an auparavant du fait d’un décalage progressif de sa mâchoire. L’examen clinique a révélé un profil convexe avec une forte contraction labiale en occlusion, une exocclusion maxillaire droite, une bascule du plan d’occlusion avec occlusion verrouillée en ciseaux, une prognathie maxillaire et une laterognathie mandibulaire. Un nivellement des arcades dentaires a été obtenu à l’aide de miniplaques d’ancrage. L’extraction de quatre prémolaires a permis de corriger la proalvéolie mandibulaire, les torques maxillaires et de coordonner les formes d’arcade. La chirurgie bimaxillaire a permis de corriger l’exocclusion droite, la prognathie
maxillaire et la latérognathie mandibulaire. On note une bonne stabilité des résultats dentaires et squelettiques à 1 an de contention.
Videoassistierte Thorakoskopie unter Erhalt der Spontanatmung – eine anästhesiologische Perspektive
Authors: Sponholz Christoph , Winkens Michael , Fuchs Frank , Moschovas Alexandros , Steinert Matthias 
Published: November 2020

Zusammenfassung
Einleitung Videoassistiere thorakoskopische Verfahren (VATS) unter Erhalt der Spontanatmung (NI-VATS) erleben derzeit weltweit eine Renaissance. Bisher liegen nur wenige Berichte über die Selektion geeigneter Patienten sowie das operative und anästhesiologische Management dieser Verfahren in Deutschland vor. In dieser Fallserie legen wir unsere bisherigen Ergebnisse und Erfahrungen zur NI-VATS dar und diskutieren die Ergebnisse anhand vorliegender Umfrageergebnisse und der verfügbaren Literatur.
Methode Retrospektive Datenerhebung aller Patienten, die in unserer Einrichtung einer NI-VATS unterzogen wurden.
Ergebnisse Im Zeitraum 06/2018 bis 1/2020 wurden 17 (9 männliche und 8 weibliche) Patienten einer NI-VATS unterzogen. Die Patienten hatten ein medianes Alter von 68 [61 – 79] Jahren. Vierzehn Patienten hatten eine fortgeschrittene Krebserkrankung, die letztendlich zur Lungenoperation führte. Alle Patienten hatten schwerwiegende Nebenerkrankungen und wurden den ASA-Klassen III (n = 9) und IV (n = 8) zugeordnet. Die Operationen waren von kurzer Dauer (im Median 18 [15 – 27] Minuten) und beinhalteten in 82% der Fälle pleurale Eingriffe (Pleurektomie, Dekortikation oder Einlage einer Thoraxdrainage). Alle Patienten tolerierten die Operation unter Lokalanästhesie und Analgosedierung. Elf Patienten konnten postoperativ direkt über den Aufwachraum auf die Normalstation verlegt werden, die restlichen Patienten wurden einer intensivierten postoperativen Überwachung zugeführt. Fünf der 17 Patienten verstarben im weiteren stationären Aufenthalt, im Median 8 [3,0 – 33,5] Tage
nach der Operation im Rahmen ihrer Grunderkrankung. Kein Todesfall konnte mit der Operationstechnik in Zusammenhang gebracht werden.
Diskussion Die NI-VATS stellt bei ausgewählten Patienten und entsprechender Expertise vor Ort eine sichere und praktikable Alternative zur Operation in Allgemeinanästhesie und Einlungenventilation dar. In unserer Einrichtung wurden multimorbide Patienten mit Eingriffen von kurzer Dauer und überschaubarem Ausmaß der NI-VATS unterzogen und kristallisierten sich als geeignete Kandidaten für dieses Verfahren heraus. Neben der sorgfältigen Patientenselektion erscheint das (Er-)Kennen häufiger Komplikationen ein wichtiger Meilenstein der erfolgreichen NI-VATS zu sein.
Erythroxylum pungens Tropane Alkaloids: GC-MS Analysis and the Bioactive Potential of 3-(2-methylbutyryloxy)tropan-6,7-diol in Zebrafish (Danio rerio)
Authors: Gondim Lambert Moreira Letícia , Leite Ferreira Maria Elisa , Reginaldo Fernanda Priscila Santos , Lourenço Estela Mariana Guimarães , Zuanazzi José Angelo Silveira , Barbosa Euzébio Guimarães , Ferreira Leandro de Santis , Fett-Neto Arthur Germano , Cavalheiro Alberto José , Luchiari Ana Carolina , Giordani Raquel Brandt 
Published: November 2020

AbstractTropane alkaloids are specialized plant metabolites mostly found in the Erythroxylaceae and Solanaceae families. Although tropane alkaloids have a high degree of structural similarity because of the tropane ring, their pharmacological actions are quite distinct. Brazil is one of the main hotspots of Erythroxylum spp. diversity with 123 species (almost 66% of the species catalogued in tropical America). Erythroxylum pungens occurs in the Caatinga, a promising biome that provides bioactive compounds, including tropane alkaloids. As part of our efforts to investigate this species, 15 alkaloids in specimens harvested under different environmental conditions are presented herein. The occurrence of 3-(2-methylbutyryloxy)tropan-6,7-diol in the stem bark of plants growing in their natural habitat, greenhouse controlled conditions, and after a period of water restriction, suggests that it is a potential chemical marker for the species. This alkaloid was evaluated for several parameters in zebrafish (Danio rerio) as a model organism. Regarding toxicity, teratogenic effects were observed at 19.5 µM and the lethal dose for embryos was 18.4 µM. No mortality was observed in adults, but a behavioral screen showed psychostimulatory action at 116.7 µM. Overall, the alkaloid was able to cause zebrafish behavioral changes, prompting further investigation of its potential as a new molecule in the treatment of depression-like symptoms. In silico, targets involved in antidepressant pathways were identified by docking.
Rapid Screening for EGFR Inhibitor in Rhei Radix et Rhizoma by HTRF Assay Coupled with HPLC Peak Fractionation
Authors: Fu Yu , Xie Zhishen , Zhao Peng , Lv Shuangshuang , Chen Suiqing 
Published: November 2020

AbstractIn this paper, an HPLC peak fractionation approach combined with homogeneous time-resolved fluorescence analysis is proposed for screening epidermal growth factor receptor inhibitors from Rhei Radix et Rhizoma. With this approach, the amount of sample used for a single HPLC run is sufficient for performing a multiple assay due to the miniaturization ability of the homogeneous time-resolved fluorescence technology. This allows for improving the stability and repeatability of the activity assay for each fraction. From a total of 26 fractions collected from the Rhei Radix et Rhizoma extract, 13 fractions exhibit inhibitory activity against the epidermal growth factor receptor. The structures of activity compounds were determined by HPLC-LTQ-Orbitrap MS, revealing the presence of gallic acid, rhein, and emodin with IC50 values of 21.5, 5.29, and 10.2 µM, respectively. The ligand epidermal growth factor receptor interactions were explored by molecular docking
simulations, and the inhibitory effects of the three compounds on A549 cell growth were tested in vitro by an MTT assay. This study demonstrates the suitability of the present screening method for drug discovery in natural products.