bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-intensity focused ultrasound enhances the cisplatin sensitivity to human non-small cell lung cancer in vitro and in vivo
Authors: Wang Liwei , Huijun Fan , Haiyan Wang , Yunlu Bai , Wenyan Zhou 
Published: July 2020

Abstract
BackgroundThe incidence and mortality of non-small cell lung cancer (NSCLC) rank first among malignant tumors worldwide. Cisplatin (CDDP) is currently the first-line chemotherapy drug used in clinical practice. However, the underlying mechanism that NSCLC cells are resistant to CDDP has not been fully elucidated. Therefore, it is urgent to explore the exact function of chemotherapy resistance and improve the anti-tumor effect of CDDP treatment.MethodWe investigated the surviving fraction of H1299 and A549 cells treated with different High-intensity focused ultrasound (HIFU) and various CDDP concentrations. HIFU (400W/cm2) and CDDP (10 μM) were selected to probe the further function in H1299 and A549 cells. Flow cytometry, MTT, and colony formation assays were performed to evaluate the apoptosis and proliferative effect of HIFU and CDDP on NSCLC cells. Transwell assays were used to analyze the migration and invasion abilities of NSCLC cells with HIFU and CDDP treatments. Platinum (Pt) accumulation was further measured in H1299 and A549 cells with different treatments. Finally, An NSCLC patient-derived xenograft model was used to explore the effect of HIFU and CDDP on NSCLC tumor growth.ResultsHIFU combined with CDDP can markedly reduce the surviving rate and promote the apoptosis of NSCLC cells. Furthermore, co-treatment with HIFU and CDDP significantly inhibited the proliferation, colony formation, migration, and invasion of NSCLC cells compared to that with any single treatment. Moreover, the combined therapy can effectively promote Pt accumulation in NSCLC cells. Further functional analysis suggested that HIFU combined with CDDP can inhibit tumor growth in an NSCLC patient-derived xenograft model. Finally, the CDDP method effectively upregulated the expression level of apoptosis-related protein, cleaved-PARP, which could be further enhanced by the HIFU treatment.ConclusionOur results revealed that HIFU enhances the anti-NSCLC effect of CDDP both in vitro and in vivo, providing a promising combination therapy for clinical NSCLC treatment.
Hydrogenotrophic denitrification for treating nitrate contaminated without/with reactive black 5 dye
Authors: Singhopon Tippawan , Shinoda Kenta , Rujakom Suphatchai , Kazama Futaba 
Published: July 2020

Abstract
NO3-N and dye colors discharged from textile wastewater pose environmental problems in Thailand. This study aimed to observe the nitrogen removal rate (NRR) with and without RB-5 color contamination via hydrogenotrophic denitrification (HD) processing, which uses H2 gas as electron donor to reduce NO3-N and NO2-N; comparing with bioreactors treatment to evaluate systems that can simultaneously remove NO3-N and dye color. Five reactors under different operation and gas supply conditions were set-up under HRT of 24 h, including an aerobic reactor using air, two anaerobic reactors using argon and H2, and a combined process using intermittent air/argon and air/H2. NRR without dye varied between 45 and 90% for H2 and air/H2 by HD processing, while it was completely removed when adding color. H2 and air/H2 reactors experienced partial decolorization of approximately 20–30%, whereas the other three reactors remained unchanged. Effluent of NO3-N were close to wastewater standards, but the color was still easy to detect, which indicated that the treatment time needs to be sufficient. In conclusion, HD and intermittent air/H2 processing can completely remove NO3-N and NO2-N when contaminated with RB-5 color. Furthermore, RB-5 did not affect the NRR, whereas some particles of dye color can also reduce in these processes.
Removal of antibiotic from the water environment by the adsorption technologies: a review
Authors: Dutta Joydeep , Mala Aijaz Ahmad 
Published: July 2020

Abstract
Antibiotics are known as emergent pollutants because of their toxicological properties. Due to continuous discharge and persistence in the aquatic environment, antibiotics are detected almost in every environmental matrix. Therefore antibiotics that are polluting the aquatic environment have gained significant research interest for their removal. Several techniques have been used to remove pollutants, but appropriate technology is still to be found. This review addresses the use of modified and cheap materials for antibiotic removal from the environment.
PHOSPHO1 Gene DNA Methylations are Associated with a Change in HDL-C Response to Simvastatin Treatment
Authors: Fan Juanlin , Cai Qianru , Zhang Di , Weinstock Justin , Qu Xiaoxiao , Jiang Shanqun 
Published: July 2020

Objective:
Our aim was to detect the effects of DNA methylations in the phosphoethanolamine/
phosphocholine phosphatase (PHOSPHO1) gene on the therapeutic efficacy of simvastatin.

Methods:
We used an extreme sampling approach by selecting 211 individuals from approximately the top and
bottom 15% of adjusted lipid-lowering response residuals to simvastatin (n=104 for the high response group and
n=107 for the low response group) from a total of 734 subjects with hyperlipidemia. They received a daily oral
dose of 20 mg simvastatin for eight consecutive weeks. DNA methylation loci at the PHOSPHO1 gene were
measured using high-throughput next-generation sequencing-based sequencing technology. Fasting serum lipids
were measured at baseline and after eight weeks of simvastatin treatment.

Results:
Mean PHOSPHO1 DNA methylation had a significant negative correlation with high-density lipoprotein
cholesterol (HDL-C) variation (β=-0.014, P=0.045) in the high response group. After stratifying by body mass
index (BMI), the associations between the PHOSPHO1 DNA methylations and the change in HDL-C in response
to simvastatin were more significant in obese subjects with a BMI of 25 kg/m2 or higher (β=-0.027, P=0.002).
Mean PHOSPHO1 methylation and traditional predictors could explain up to 24.7% (adjusted R2) of the change
in HDL-C response in obese patients. There was a statistically significant additive interaction term (P=0.028)
between BMI and mean PHOSPHO1 methylation in the model of the change in HDL-C in response to simvastatin.

Conclusion:
Our findings suggest that PHOSPHO1 DNA methylations are associated with a change in HDL-C in
response to simvastatin treatment, and this association is especially dependent on the extent of patient obesity.
Targeting Toll like Receptors in Cancer: Role of TLR Natural and Synthetic Modulators
Authors: Narayanankutty Arunaksharan , Sasidharan Aswathi , Job Joice T. 
Published: July 2020

Background:Toll like receptors (TLRs) are a group of transmembrane receptors belonging to the broad class pattern recognition receptors (PRR), involved in recognition of Pathogen Associated Molecular Patterns (PAMPs) thereby inducing an immune response. Apart from these exogenous PAMPs, numerous endogenous PAMPs are also ligands for various TLRs thereby activating the TLR dependent immune response, subsequently leading to the onset of an inflammatory response. Prolonged activation of TLR by these endogenous PAMPs leads to chronic inflammatory insults to the body and which in turn alters the proliferative patterns of the cells, which ultimately leads to the development of cancer.Objectives:The present review aims to provide a detailed outline of the differential roles of various TLRs in cancer and the possible use of them as a therapeutic target.Methods:Data were collected from PubMed/Sciencedirect/Web of Science database and sorted; the latest literature on TLRs was incorporated in the review.Results:Among the different TLRs, few are reported to be anti-neoplastic, which controls the cell growth and multiplication in response to the endogenous signals. On the contrary, numerous studies have reported the procarcinogenic potentials of TLRs. Hence, TLRs have emerged as a potential target for the prevention and treatment of various types of cancers. Several molecules, such as monoclonal antibodies, small molecule inhibitors and natural products have shown promising anticancer potential by effectively modulating the TLR signalling.Conclusion:Toll-like receptors play vital roles in the process of carcinogenesis, hence TLR targeting is a promising approach for cancer prevention.
Preconcentration and Determination of Clomiphene Citrate Using Hollow- Fiber Liquid-phase Mixroextraction Coupled with HPLC-UV Detection
Authors: Ziyarati Mehdi , Qomi Mahnaz , Yousefi Mohammad 
Published: July 2020

Background:
Currently, ovulation stimulation with clomiphene citrate is the most commonly used treatment in
infertility. It stimulates the secretion of gonadotropins necessary for the release of an egg.

Objective:
In this study, a microextraction method of liquid phase was utilized using hollow fiber with high-performance
liquid chromatography and ultraviolet (HPLC-UV) detector for pre-concentration and measuring clomiphene citrate in
plasma.

Method:
Clomiphene citrate was extracted from a 15 ml sample solution with pH=11 in an organic solvent (Octanol),
which was located in the vent wall of the fiber. Subsequently, the drug was introduced into an internal organic solvent into
an acidic pH=2 receptor that was located inside the fiber. In the following, effective factors in microextraction including
pH of the donor phases and acceptor phases, type of organic solvent, ion phase strength, extraction time and stirring speed
were investigated and optimized.

Results:
After extracting the drug with an optimal pre-concentration factor of 212, extraction recovery was 91%, the detection limit was 1.50 ng/ml, limiting the linearity of 5-1000 ng/mL and %RSD=6.98.

Conclusion:
The proposed method was successfully implemented and optimized for trace detection of clomiphene citrate
in plasma, therefore the clinical and investigational application of this method is facilitated by this study.
hGWO-SA: A Novel Hybrid Grey Wolf Optimizer-Simulated Annealing Algorithm for Engineering and Power System Optimization Problems
Authors: Kamboj Vikram Kumar 
Published: July 2020

:
The improved variants of a Grey wolf optimizer have good exploration capability for the
global optimum solution. However, the exploitation competence of the existing variants of grey
wolf optimizer is unfortunate. Researchers are continuously trying to improve the exploitation
phase of the existing grey wolf optimizer, but still, the improved variants of grey wolf optimizer
lack in local search capability. In the proposed research, the exploitation phase of the existing grey
wolf optimizer has been further improved using a simulated annealing algorithm and the proposed
hybrid optimizer has been named as hGWO-SA algorithm. The effectiveness of the proposed hybrid
variant has been tested for various benchmark problems, including multi-disciplinary optimization
and design engineering problems and unit commitment problems of the electric power system
and it has been experimentally found that the proposed optimizer performs much better than existing
variants of grey wolf optimizer. The feasibility of hGWO-SA algorithm has been tested for
small & medium scale power systems unit commitment problems, in which, the results for 4 unit, 5
unit, 6 unit, 7 unit, 10 units, 19 unit, 20 unit, 40 unit and 60 units are evaluated. The 10-generating
units are evaluated with 5% and 10% spinning reserve. The results obviously show that the
suggested method gives the superior type of solutions as compared to other algorithms.
A Note on Estimating the Case Fatality Ratio for Novel Corona Virus Disease (COVID-19)
Authors: Swain Prafulla Kumar 
Published: July 2020

Background:
In this paper, an attempt has been made to estimate the Case Fatality Ratio
(CFR) for coronavirus disease of India and a few selected countries. And also, it highlighted the pros
and cons of obtaining crude and adjusted CFR of COVID-19 pandemic.

Material and Methods:
Data extracted from the WHO situation report and the University of Oxford
website have been used for this analysis. The CFR and its 95% confidence interval were computed,
trend and bar plot was used for graphical representation.

Results:
The worldwide crude CFR stands 6.73% (95% CI 6.69 to 6.76) based on 21, 83, 877 confirmed
and 1,46,872 death cases(as on 17th April, 2020). Belgium was the highest CFR at 13.95% as compared
to others. However, India’s CFR was found to be around 3.26% (as on 17th April, 2020).

Conclusion:
In conclusion, the estimation and interpretation of CFR are critical in response to ongoing
COVID-19. The initial CFR estimates are subject to change, still, it is useful for healthcare planning
over the coming months. Moreover, the precise and robust estimates of CFR will be available only at the
end of the epidemic.
Pencapaian Standar Kompetensi Siswa SMK Negeri Program Keahlian Tata Busana di Kota Yogyakarta dalam Pembelajaran dengan KBK
Authors: Widihastuti Widihastuti 
Published: July 2020

Artikel ini ditulis berdasarkan hasil penelitian evaluasi keefektifan pembelajaran pada penerapan KBK di Program Keahlian Tata Busana Kota Yogyakarta. Penelitian ini merupakan penelitian evaluasi dengan pendekatan kuantitatif. Populasi penelitian meliputi 671 siswa Program Keahlian Tata Busana SMK Negeri di Kota Yogyakarta yang telah melaksanakan KBK. Sampel penelitian sebanyak 137 siswa yang ditentukan dengan teknik proportional stratified random sampling menggunakan rumus Krejcie & Morgan. Data penelitian diperoleh dari dokumen nilai prestasi akademik siswa, yang meliputi nilai normatif, adaptif, dan produktif. Teknik analisis menggunakan statistik deskriptif dan inferensial dengan bantuan komputer paket program SPSS 10 for windows. Hasil penelitian menunjukkan bahwa: pembelajaran pembentukan kompetensi normatif cukup efekti£ dan pembentukan kompetensi adaptif dan produktif kurang efektif. Kata kunci: pencapaian standar kompetensi siswa
PENGARUH STRATEGI PEMBELAJARAN DAN KEMAMPUAN ANALITIK TERHADAP KETERAMPILAN PNEUMATIK MAHASISWA TEKNIK MESIN UNY
Authors: Triyono M. Bruri 
Published: July 2020

Penelitian ini bertujuan untuk mengetahui perbedaan keterampilan pneumatik pada mahasiswa yang diajar dengan strategi pembelajaran lengkap dan demonstrasi sesuai berdasarkan kemampuan analitiknya pada pembelajaran mata kuliah Pneumatik Hidroulik. Penelitian ini merupakan penelitian quasi eksperimen dengan variabel terikat keterampilan pneumatik, variabel bebas strategi pembelajaran, dan variabel atribut kemampuan analitik mahasiswa. Rancangan eksperimen faktorial 2x2 dengan dua ta-raf kualifikasi variabel bebas yaitu strategi pembelajaran lengkap dan demonstrasi. Dua kelas sebagai subyek penelitian dengan 32 mahasiswa pada masing-masing kelas, sehingga jumlah keseluruhan adalah 64 mahasiswa yang dipilih secara random. Temuan penelitian ini adalah (1) keterampilan pneumatik mahasiswa yang diajar dengan strategi lengkap lebih tinggi dibanding yang diajar dengan strategi demonstrasi, (2) kelompok mahasiswa yang berkemampuan analitik tinggi dan diajar dengan strategi lengkap, keterampilannya lebih tinggi dibanding yang diajar dengan strategi demonstrasi, (3) kelompok mahasiswa yang berkemampuan analitik rendah dan diajar dengan strategi demonstrasi, keterampilannya lebih tinggi dibanding yang diajar dengan strategi lengkap, (4) ada interaksi antara strategi pembelajaran dan kemampuan analitik terhadap keterampilan pneumatik. Kata kunci: strategi pembelajaran, kemampuan analitik, keterampilan pneumatik
PENGARUH TES FORMATIF DAN KEMANDIRIAN BELAJAR TERHADAP HASIL BELAJAR EKONOMI SISWA SMA
Authors: A. Ghani Abdul Rahman 
Published: July 2020

Tujuan penelitian ini adalah untuk menguji ada tidaknya pengaruh tes formatif dan kemandirian belajar terhadap hasil belajar siswa dalam mata pelajaran Ekonomi. Penelitian ini menggunakan metode eksperimen dengan desain faktorial 2x2. Eksperimen dilaksanakan pada tahun pelajaran 2004/2005. Subjek penelitian adalah siswa Kelas II semester I SMA Negeri 63 dan 29 di Jakarta Selatan. Jumlah sampel 104 siswa dipilih secara acak bertahap. Eksperimen dilakukan melalui pemberian tes formatif bentuk esai dan pilihan ganda sebanyak 7 kali. Hasil penelitian menunjukkan bahwa: (1) Penggunaan tes formatif bentuk esai dalam proses pembelajaran dapat memberikan hasil belajar yang lebih tinggi dibanding dengan penggunaan tes formatif bentuk pilihan ganda. (2) Bagi siswa yang memiliki kemandirian belajar tinggi, penggunaan tes formatif bentuk esai dalam evaluasi formatif dapat memberikan hasil belajar yang lebih tinggi dibanding dengan penggunaan tes formatif bentuk pilihan ganda. (3) Bagi siswa yang memiliki kemandirian belajar rendah, penggunaan tes formatif bentuk esai dan bentuk pilihan ganda tidak memberikan hasil belajar yang berbeda. (4) Efektivitas penggunan tes esai dan pilihan ganda dalam evaluasi formatif sangat tergantung pada tingkat kemandirian belajar siswa, pada tingkat kemandirian belajar tinggi lebih efektif digunakan tes formatif bentuk esai dan pada tingkat kemandirian belajar rendah lebih efektif digunakan tes formatif bentuk pilihan ganda. Kata kunci: hasil belajar ekonomi, tes formatif, kemandirian belajar
MODEL PEMBELAJARAN LIMA DOMAIN SAINS DENGAN PENDEKATAN KONTEKSTUAL UNTUK MENGEMBANGKAN PEMBELAJARAN BERMAKNA
Authors: Rosana Dadan 
Published: July 2020

Penelitian ini bertujuan untuk mengembangkan Model Pembelajaran Lima Domain Sains (MP5DS) dengan pendekatan kontekstual berlandaskan teori konstruktivisme untuk menghasilkan pembelajaran bermakna, melalui tahapan; (1) pengembangan perangkat pembelajaran dan sumber belajar (2) pengujian hubungan antar variabel, serta MP5DS dengan variabel yang mempengaruhinya. Metode penelitian mengggunakan penelitian dan pengembangan model spiral. Teknik analisis yang digunakan adalah analisis jalur dan model persamaan struktural menggunakan AMOS 6.0. Hasil penelitian ini menunjukkan: (1) prototipe perangkat pembelajaran dan prosedur implementasi MP5DS untuk pembelajaran sains, (2) terdapat pengaruh langsung yang positif dan signifikan maupun tidak langsung antar variabel kognitif, afektif, proses, aplikasi dan kreativitas, (3) pencapaian nilai tes kognitif pada kategori tinggi (73,11%), nilai sikap cukup baik (58%), nilai proses tinggi (63,31%), nilai aplikasi tinggi (61,93%) dan nilai kreativitas masih rendah (39,41%), serta (4) MP5DS dipengaruhi oleh lingkungan psikososial pembelajaran sains dan kompetensi guru. Variabel lingkungan psikososial pembelajaran sains memiliki pengaruh langsung positif dan signifikan (faktor bobot = 0,60, cr=11,490, dan p<0,000|), tetapi variabel kompetensi guru pengaruhnya tidak signifikan (faktor bobot = 0,34, cr=11,490, dan p<0,000|). Kata kunci: MP5DS, kontekstual, pembelajaran bermakna
PSIKOLOGI KOGNITIF SEBAGAI DASAR PENGEMBANGAN TES KEMAMPUAN DASAR MEMBACA BIDANG SAINS
Authors: Rusilowati Ani 
Published: July 2020

Penelitian ini bertujuan untuk: (1) menguji psikologi kognitif sebagai dasar pengembangan tes kemampuan dasar (TKD) membaca sains; (2) menentukan karakteristik TKD membaca sains yang dikembangkan; (3) menentukan kriteria penilaian dan cara menginterpretasikan hasil TKD. Penelitian ini merupakan penelitian pengembangan. Populasi penelitian adalah siswa SD kelas 5 di kota Ungaran, kabupaten Semarang. Sampel ditentukan secara clustering random sampling, dengan ukuran 549 siswa. Instrumen berupa tes kemampuan dasar membaca, bertema Sains, berbentuk tes objektif 3 pilihan, sebanyak tiga buku tes. Analisis dilakukan dengan bantuan software microCat ITEMAN, BILOG, dan LISREL. Hasil penelitian berupa: (1) tiga buku tes TKD-membaca yang telah diketahui karakteristik soalnya dan (2) TKD-meter sebagai alat interpretasi hasil tes kemampuan dasar membaca. Karakteristik soal TKD-membaca meliputi indeks tingkat kesukaran (berada pada kategori mudah sampai sukar) dan indeks daya beda (berada pada kategori baik). TKD-meter yang dihasilkan terdiri atas empat level. Level I merupakan level terendah, dan level IV merupakan level tertinggi. Siswa berada pada level I, setara dengan kemampuan siswa kelas 3 SD. Siswa berada pada level II, setara dengan kemampuan siswa kelas 4 SD. Kata kunci: tes kemampuan dasar membaca, sains, psikologi kognitif
Barriers and Facilitators to Implementation of Medication Decision Support Systems in Electronic Medical Records: Mixed Methods Approach Based on Structural Equation Modeling and Qualitative Analysis
Authors: Jung Se Young , Hwang Hee , Lee Keehyuck , Lee Ho-Young , Kim Eunhye , Kim Miyoung , Cho In Young 
Published: July 2020

Background
Adverse drug events (ADEs) resulting from medication error are some of the most common causes of iatrogenic injuries in hospitals. With the appropriate use of medication, ADEs can be prevented and ameliorated. Efforts to reduce medication errors and prevent ADEs have been made by implementing a medication decision support system (MDSS) in electronic health records (EHRs). However, physicians tend to override most MDSS alerts.

Objective
In order to improve MDSS functionality, we must understand what factors users consider essential for the successful implementation of an MDSS into their clinical setting. This study followed the implementation process for an MDSS within a comprehensive EHR system and analyzed the relevant barriers and facilitators.

Methods
A mixed research methodology was adopted. Data from a structured survey and 15 in-depth interviews were integrated. Structural equation modeling was conducted for quantitative analysis of factors related to user adoption of MDSS. Qualitative analysis based on semistructured interviews with physicians was conducted to collect various opinions on MDSS implementation.

Results
Quantitative analysis revealed that physicians’ expectations regarding ease of use and performance improvement are crucial. Qualitative analysis identified four significant barriers to MDSS implementation: alert fatigue, lack of accuracy, poor user interface design, and lack of customizability.

Conclusions
This study revealed barriers and facilitators to the implementation of MDSS. The findings can be applied to upgrade MDSS in the future.
GENOTOXICITY EVALUATION OF CISSUS LATIFOLIA LAM. AND ITS GENOPROTECTIVE EFFECT ON OXIDATIVE DAMAGE INDUCED BY HYDROGEN PEROXIDE
Authors: M RUBEENA , E THOPPIL JOHN 
Published: July 2020

Objective: This study focused on the genotoxic efficacy of higher concentrations of methanolic extract of Cissus latifolia Lam. and its genoprotective role at lower concentrations against the induced cytogenetic aberrations by 2% hydrogen peroxide [H2O2] on the Allium cepa plant model.
Methods: Cytotoxic and genoprotective potential of the plant extract were analyzed using A. cepa assay. The treatment modes that were designed for antigenotoxicity screening were pre-treatment, post-treatment, and concurrent treatment.
Results: Results revealed the toxic effect of extract of C. latifolia which induced major chromosomal abnormalities, namely, nuclear lesions, chromosome vagrants, stickiness, bridges, and chromosome breaks. These aberrations were found to be unaltered and showed the potent clastogenic nature of plant extract. As concentrations of plant extract and exposure time increase, the frequency of occurrence of cytogenetic aberrations was also detected to be more. Further, the potential genotoxicity of plant measured in terms of mitotic index reduction also confirms its significant role in inducing mutagenicity. The genoprotective efficacy of extract was identified as its ability to inhibit and reverse the aberrations caused by H2O2 at lower concentrations of plant extract.
Conclusions: Dose-dependent data highlight the cytotoxic and genoprotective effect of C. latifolia on the A. cepa model. These findings will help to exploit the plant as an anticancer drug and explore other wide arrays of therapeutic activities of C. latifolia.