bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Actinidia chinensis (kiwifruit) eFP browser and network analysis of transcription factors
Authors: Brian Lara , Warren Ben , McAtee Peter , Rodrigues Jessica , Nieuwenhuizen Niels , David Karine M. , Richardson Annette , Allan Andrew C. , Varkonyi-Gasic Erika , Schaffer Robert J 
Published: November 2020

Abstract
BackgroundTranscriptomic studies combined with a well annotated genome have laid the foundations for new understanding of molecular processes. Tools which visualise gene expression patterns have further added to these resources. The manual annotation of the Actinidia chinensis (kiwifruit) genome has resulted in a high quality set of 33,044 genes. Here we investigate gene expression patterns in diverse tissues, visualised in an Electronic Fluorescent Pictograph (eFP) browser, to study the relationship of transcription factor (TF) expression using network analysis. ResultsSixty-one samples covering diverse tissues at different developmental time points were selected for RNAseq analysis and an eFP browser was generated to visualise this dataset. 2,839 TFs representing 57 different classes were identified and named. Network analysis of the TF expression patterns separated TFs into 14 different modules. Two modules consisting of 237 TFs were correlated with floral bud and flower development, a further two modules containing 160 TFs were associated with fruit development and maturation. A single module of 480 TFs was associated with ethylene-induced fruit ripening. Three “hub” genes correlated with flower and fruit development consisted of a HAF-like gene central to gynoecium development, an ERF and a DOF gene. Maturing and ripening hub genes included a KNOX gene that was associated with seed maturation, and a GRAS-like TF.ConclusionsThis study provides an insight into the complexity of the transcriptional control of flower and fruit development, as well as providing a new resource to the plant community. The eFP browser is provided in an accessible format that allows researchers to download and work internally.
Genome-wide Identification of Gramineae Histone Modification Genes and their Potential Roles in Regulating Wheat and Maize Growth and Stress Responses
Authors: Zheng Liwei , Ma Shengjie , Shen Dandan , Fu Hong , Wang Yue , Liu Ying , Shah Kamran , Yue Caipeng , Huang Jinyong 
Published: November 2020

Abstract
Background: In pants, histone modification (HM) participates in various developmental and defenses processes. Gramineae plants were important crop species worldwide. However, little information on them is in gramineae species. Results: In six gramineaes, 245 TaHMs, 72 HvHMs, 84 SbHMs, 93 SvHMs, 90 SiHMs and 90 ZmHMs were respectively identified. Their detailed information, including chromosome locations, conserved domains, phylogenetic trees, synteny, promoter elements and gene structures, were identified. Among these HMs, most of motifs were conservative, while unique ones were also identified. Gene and genome duplications may result in the evolution and expansion of HMs in wheat. The number of gene pairs between rice and each gramineae was much greater than that between Arabidopsis and each gramineae, which indicated dicotyledons sharing common ancestors. Moreover, all identified HMs gene pairs may undergo purifying selection according to their Ka/Ks values. Expression profiles of TaHMs in developing wheat grain, responding to brassinosteroid, brassinazole as well as activated charcoal were investigated in published transcriptome data, and transcription models of ZmHMs in maize development seeds and after gibberellin treatment were also identified. In addition, heat, drought, salt, insect feeding, nitrogen and cadmium stresses influenced many TaHMs, and drought altered several ZmHMs expression. These findings indicated their important functions in plant growth and stress adaptions. Conclusion: In conclusion, a comprehensive analysis of six gramineae HM gene families was completed; TaHMs were likely to participate in grain development, brassinosteroid- as well as brassinazole-mediated root growth, activated charcoal-mediated root and leaf growth, and biotic and abiotic adaptions; ZmHMs take part in seed development, gibberellin-mediated leaf growth, and drought adaption.
Early Breastfeeding Cessation Among Post-partum Women: A Prospective Observational Study
Authors: Shahrani Abeer Salem Al , Hushan Hessah Mamdouh , Binjamaan Noura Khalid , Binhuwaimel Wajd Abdulrahman , Alotaibi Jawaher Jazaa , Alrasheed Lama Ali 
Published: November 2020

Abstract
Background: The World Health Organization (WHO) recommends that breastfeeding is initiated within an hour of birth and breastfeeding exclusively is performed for the first six months. However, the exclusive breastfeeding rate is dropping in most countries. In this study, we aimed to assess the breastfeeding cessation rate during the early post-partum period. Moreover, we aimed to prospectively identify the risk factors for early breastfeeding cessation during hospitalization and after discharge.Methods: This prospective study included all post-partum women who had given birth to full term, singleton, and healthy newborns and were breastfeeding at discharge from Mar – Jun 2020 at King Abdullah bin Abdulaziz University Hospital (KAAUH) in Riyadh, Saudi Arabia. With an instrument that was designed based on previous studies, data were collected at the time of admission and 10 – 14 days and 6 – 8 weeks post-partum.Results: This study included 136 mothers. The mean age of the participants was 29.5 ±5.07 years, and the majority of the participants had a bachelor’s degree and were unemployed. A total of 37.5% of the participants were exclusively breastfeeding their newborns for the first two weeks; however, this rate later dropped to 19%. However, bottle feeding was performed by two-thirds of the participants for the first 2 weeks, and this rate increased to 80.9% at 6 – 8 weeks post-partum. Many factors, such as maternal age, the mode of delivery and previous breastfeeding experience, were strongly associated with breastfeeding. Bottle feeding was associated with maternal age as well as employment status.Conclusions: This study reported an unacceptably low exclusive breastfeeding rate within the first two months post-partum. Multiple factors were strongly associated with breastfeeding, and some of them were modifiable. Moreover, more attention should be given to mothers who have a high risk for early breastfeeding cessation, such as employed mothers.Trial registration: Not applicable
Trends in Breastfeeding Practices and Mothers’ Experience in the French Nutrinet-Santé Cohort.
Authors: Courtois Frederic , Péneau Sandrine , Salavane Benoît , Andreeva Valentina A , Roland-Cachera Marie-Françoise , Touvier Mathilde , Galan Pilar , Hercberg Serge , Fezeu Léopold K 
Published: November 2020

Abstract
BackgroundDespite the suggested health benefits of breastfeeding for infants and mothers,France has one of the lowest rates regarding breastfeeding initiation and duration in the world. Fewstudies have been performed concerning the extent of breastfeeding practices in France since themiddle of the 20th century, or regarding the mothers’ practices from initiation to cessation. Thepurpose of our study is to determine trends in breastfeeding over the past decades and to examinemothers’ perceptions about factors known to have an impact on breastfeeding support and cessation.Methods29,953 parous women from the French NutriNet-Santé cohort were included in theprsent study. Using web-questionnaires, they were asked retrospectively if they breastfed or nottheir youngest child, and if so, the duration of the exclusive and total breastfeeding. For those whohad breastfed, we investigated their perceptions about support at initiation and during the entirebreastfeeding period and the reasons for breastfeeding cessation. We also asked those who did notbreastfeed about their perceptions on non-breastfeeding their youngest child and the reasons forchoosing to bottle-fed him or her. Analyses were weighted according to the French census data.Results67.3% of mothers in the NutriNet-Santé cohort breastfed their youngest child. Theproportion of breastfed children increased tover the past few decades, from 55.0% (95%CI: 54.3 –55.6) in the 1970s to 82.9% (82.4 – 83.4) in the 2010s. Mean total breastfeeding duration was 4.7months (4.5 – 5.0) while exclusive breastfeeding duration was 2.8 months (2.7 – 2.9). Mothers feltsupported at initiation and over the breastfeeding period. 59.5% of mothers reported a desire to havebreastfed more than 2 months longer. Mothers who did not breastfeed did it by choice (64.3%).They did not feel guilty (78.2%) and did not perceive a problem not to breastfeed (58.8%), butalmost half of them would have liked to breastfeed (45.9%).ConclusionBreastfeeding duration has increased in the past decades but did not reach the publichealth recommendations threshold. Other targets than mothers have to be considered, like the fatherand her environment, to increase breastfeeding practices.Trial RegistrationThe study was registered at ClinicalTrials.gov (NCT03335644).
Retrospective Study of Frequency of ABO and Rhesus Blood Group Among Population of Safdarabad and Faisalabad Cities of Pakistan
Authors: Sabir Ali , Iftikhar Arslan , Ijaz Muhammad Umar , Hussain Ghulam , Rasul Azhar , Iqbal Rana Khalid , Sajid Faiqa , Anwar Haseeb 
Published: November 2020

Abstract
Objective:The current study aimed to investigate the ABO and rhesus (Rh) blood group frequency in the people of District Faisalabad and Sheikhupura, Punjab Province, Pakistan. The retrospective study was conducted on more than thirty thousand people including both male and female patients admitted to the Tehsil Headquarter Hospital, Safdarabad and The Best Hospital, Faisalabad. Blood samples were taken from each subject and subsequently ABO and Rh blood groups were evaluated separately. The antigen antibody agglutination slide test for blood grouping (ABO) and Rh were used to assess the blood group frequencies. Results:The frequencies of ABO blood group distribution indicated that blood group B was predominant in the people of Safdarabad followed by O, A and AB respectively. While, among people of Faisalabad, blood group O was predominant followed B, A and AB respectively. Rh negative phenotype was found lesser distributed as compared to the positive Rh phenotype.
Prevalence and Predictors of Anxiety, Depression, and Insomnia among Healthcare Workers in Dhaka City amid COVID-19 Pandemic
Authors: Ali Mohammad , Uddin Zakir , Ahsan Nawara Faiza , Haque Muhammad Zahirul , Bairagee Monisha , Khan Sabbir Ahmed , Hossain Ahmed 
Published: November 2020

Abstract
Objective: The purpose of the study was to investigate the prevalence of anxiety, depression, and insomnia symptoms, and identify predicting factors among healthcare workers (HCW) of the Dhaka city in Bangladesh during the COVID-19 outbreak. The Generalized Anxiety Disorder 2-item, the Patient Health Questionnaire 9-item depression module, the Insomnia Severity Index were used to measure anxiety, depression and insomnia symptoms, respectively. Results: We found the prevalence of anxiety, depression and insomnia symptoms among HCW 20.7%, 26.5% and 44.2% respectively. Three regression models were run to identify the predictors of anxiety, depression and insomnia. Model 1 suggested that the singular marital status (β= -0.178, p= 0.011), occurrence of chronic disease (β= -0.132, p= 0.025), and financial difficulty (β= -0.163, p= 0.005) were the risk factors of anxiety. From model 2, it was found that the risk factors of depression were singular marital status (β= 0.254, p= <0.001) and financial difficulty (β= 0.198, p= 0.001). Final model revealed that the risk factors of insomnia were gender (β= -0.151, p= 0.008), singular marital status (β= -0.165, p= 0.016), financial difficulty (β= 0.213, p= <0.001) and occupation (β= -0.125, p= 0.029).
In Vitro Cytotoxicity of Aspilia Pluriseta Schweinf. Extract Fractions
Authors: Njeru Sospeter Ngoci , Muema Jackson Mbithi 
Published: November 2020

Abstract
Objectives: We and others have shown that Aspilia pluriseta is associated with various biological activities. However, there is a lack of information on A. pluriseta cytotoxicity. This has created an information gap about the safety of A. pluriseta extracts. As an extension to our recent publication on the antimicrobial activity and the phytochemicals characterization of A. pluriseta root extracts, here we report the missing data on cytotoxicity of tested extracts. We evaluated the potential cytotoxicity of the root extracts on Vero cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: We show that all solvent extracts (except methanolic solvent fractions) had cytotoxic concentration values that killed 50% of the Vero cells (CC50) greater than 20 µg/mL and selectivity index (SI) of greater than 1.0. Taken together, we demonstrate that, A. pluriseta extract fractions’ earlier reported bioactivity are within the acceptable cytotoxicity and selective index limits. This scientifically validates the potential use of A. pluriseta in the discovery of safe therapeutics agents.
Measuring Adherence to ARVs among HIV-positive Adolescents in Cameroon: A Comparative Assessment of Self-report and Medication Possession Ratio Methods
Authors: Bongfen Mbuwir Charlotte , Torpey Kwasi , Ganle John , Augustine Ankomah 
Published: November 2020

Abstract
ObjectiveAdherence to ARV medications has been shown to improve treatment outcomes in HIV positive patients. Given that ARV treatment is lifelong, adherence has become a critical issue as it may reduce over time. Measuring adherence is therefore imperative in programming. There are different methods of measuring adherence each with its advantages and disadvantages, depending on the context and the time. This study therefore compares two widely used adherence measurement scales in Cameroon, namely, the self-report and the Medication Possession Ration (MPR) methods.ResultsThe study shows that adherence level using the self-report technique is 82.9% while that of MPR was 73.4%. When compared using the using Kappa statistics, there was substantial agreement between the two scales of 66% (p=0.54). The results of both self-report adherence and MPR were also compared with viral load suppression and the difference between viral load suppression and MPR was significant (p<0.01). The difference in adherence between viral load suppression and the self-report measure also showed to be significant (p<0.01).
Antimicrobial Sensitivity Patterns of Staphylococcus Species Isolated from Mobile Phones and Implications in the Health Sector
Authors: Likhovole Clement , Achok Caroly , Nyaswa Philip , Ogwai Susan , Aroko Arthur , Obila James , Koigi George , Ridhwana Mustafa , Okwayo Bildad , Wanjiru Dorcas , Lukeba Linda , Ryckaert Eline , Vandurme Arne , Walschaerts Verena , De Preter Vicky 
Published: November 2020

Abstract
Objectives: The aim of this research was to determine drug sensitivity profiles of Staphylococcus species isolated from mobile phones of students of Microbiology and Biomedical Laboratory Sciences from UZIMA University of Health and Social Sciences, Kisumu (Kenya) and the University Colleges Leuven-Limburg, Leuven (Belgium), respectively. Results: All mobile phones (16/16,100%) had Gram-positive bacteria. 3/8 (37.5%) mobile devices had Staphylococcus aureus. 2/3 (67%) Staphylococcus aureus strains were resistant to ampicillin, oxacillin, ceftazidime, vancomycin and amoxicillin. Guidelines for disinfection of mobile phones need to be developed urgently to stop transmission of resistant bacteria.
Characteristics of Patients Infected With Clostridioides Difficile at a Saudi Tertiary Academic Medical Center and Assessment of Antibiotic Duration
Authors: Alammari Khadijah M. , Thabit Abrar K. 
Published: November 2020

Abstract
BackgroundClostridioides difficile infection (CDI) is a common hospital-associated diarrhea. Several antibiotics commonly associate with CDI; however, limited data are available on the duration of exposure prior to CDI development. Moreover, studies on the characteristics of CDI patients in Saudi Arabia are limited. Therefore, the objective of this study was to characterize CDI patients identified over 10 years and assess antibiotic days of therapy (DOT) prior to CDI.MethodsThis was a retrospective descriptive analysis of CDI patients identified via laboratory testing at a Saudi tertiary academic medical center between December 2007-January 2018. Patients characteristics, prior exposure to known CDI risk factors, and DOT of antibiotics prior to CDI incidence were assessed.ResultsA total of 162 patients were included. Median age was 61.5 years. Most cases were hospital-acquired (70.4%) and admitted to general medical wards (81.5%). Prior exposure to antibiotics and acid suppression therapy were reported with the majority (75.9 and 75.3%, respectively). The most frequently prescribed antibiotics were piperacillin/tazobactam, ceftriaxone, meropenem, and ciprofloxacin with median DOTs prior to CDI incidence of 16.5, 16, 16, and 28 days, respectively. The distribution of DOT was significantly different for piperacillin/tazobactam, ceftriaxone, and ciprofloxacin in different units (P < 0.05). Counterintuitively, patients in non-ICU wards had the shortest antibiotic exposure prior to CDI development.ConclusionAs CDI is a common hospital-acquired infection resulting mainly from antibiotic exposure, results from this study indicate the need to revise antibiotic therapy to assess necessity and discontinue it when deemed unnecessary within the first two weeks.
Psychotherapeutic Interventions Among Muslims Undergoing Treatment for Cancer: An Integrative Review
Authors: Khait Abdallah Abu , Lazenby Mark 
Published: November 2020

Abstract
Background: Cancer is a global disease that affects all populations, including Muslims. Psychological and spiritual care of Muslim patients with cancer includes psychotherapeutic techniques that help to improve their mental health and spiritual well-being. Although these techniques are available to cancer patients worldwide, they are poorly studied among Muslim patients. Purpose: To integrate the literature on the psychosocial-spiritual outcomes of and perspectives of Muslim patients with cancer who have undergone psychotherapy. Methods: We used the Whittemore and Knafl five-step methodology. We conducted a comprehensive search of PubMed, CINAHL, and PsycINFO using relevant keywords. Studies that focused on adult patients with cancer and on published evidence of using psychotherapeutic interventions among Muslim patients were included. Each study was reviewed, evaluated, and integrated.Results: A systematic search produced 18 studies that were thematically analyzed. Results showed different psychotherapeutic approaches currently used to care for Muslim patients with cancer that target mainly patients’ mental health, quality of life, and spiritual well-being. Four major themes emerged: (1) Treating Psychological Distress Without Psychopharmacologic Agents, (2) Improving Knowledge of Cancer for Improving QOL, (3) Depending on Faith for Spiritual Well-being, and (4) Relying on Religious and Spiritual Sources: Letting Go, Letting God. Conclusion: The rigor of psychotherapeutic studies that target psychosocial-spiritual outcomes of Muslim cancer patients needs to be improved to reach conclusive evidence about their efficacy in this population.
Arm Activity Measure (ArmA): Psychometric Evaluation of the Swedish Version
Authors: Ramström Therese , Bunketorp-Käll Lina , Wangdell Johanna 
Published: November 2020

Abstract
Spasticity after an injury to the central nervous system (CNS) can cause profound disability (1). The prevalence of spasticity differs among various diagnoses, depending on how it is defined. Spasticity is reported to be present in 80% of patients with spinal cord injury (SCI) (2, 3), 60% of patients with traumatic brain injury (TBI) (4), and 30% of patients with stroke (5, 6). The consequences of spasticity in the upper limb (UL) range from reduced grip control to a clenched fist and can prevent prehension and grasp, which are critical for independence in activities of daily living (ADLs) (7). Left untreated, spasticity can lead to severe contractures, deformity, pain, and involuntary movement and severely compromise occupational performance [3, 9-11]. Since being active is fundamentally important for all living beings, and participation in activities is necessary for human physical and mental wellbeing (8), disabling UL spasticity can have devastating consequences.
A Cohort Study on Dietary Habits, Lifestyles and the Risk of Mortality in the Elderly: the Latest Evidence From China Diet Cohort Study of Elderly Chinese
Authors: Zhou Qianyu , Qin Ying , Zhang Yanru , Sun Nan , Jia Shanqun , Liu Huimin , Liu Dandan , Li Zihao , Sun Changqing 
Published: November 2020

Abstract
Aim: To examine the longitudinal relationship between dietary habits, lifestyle factors and the risk of mortality among the elderly Chinese. Methods: Four follow-up survey data of the Chinese Longitudinal Healthy Longevity Survey 2008-2018 were selected, and a total of 11899 elderly people were included. Food habits were assessed using an in-person interview. The frequency of consumption of each food item was recorded as “almost every day”, “occasionally” or “rarely or never”. After descriptive statistics of the data, the effects of dietary habits and lifestyle factors on all-cause mortality were analyzed using the Cox proportional hazards model and the adjusted model. Results: There were 9461 deaths during 55312 person-years of follow-up with an average age of 75 years (S.D. ±8.3) years for survivors and 91.09 (S.D. ±9.6) years for non-survivors. Type of staple food was associated with a reduced risk of mortality. Compared to those who rarely/never consumed fruit, vegetables, fish, tea, and nut products, participants consuming such products almost every day were associated with 3.7%, 20.9%, 10.8%, 0.1%, and 11.7% reductions in the risk of mortality, respectively. Compared with lard, long-term use of vegetable grease and gingili grease increased the risk of mortality by 10.5% and 11.6%, respectively. Conclusions: The Chinese elderly could gain health beneﬁt from regular consumption of fruit, vegetables, ﬁsh, tea and nut products, and they should pay attention to the choice of cooking oils, reduce cooking oils frequency. Future research is warranted to establish the recommended daily nutrient allowances for the elderly and better address the nutritional needs of this vulnerable population.