bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of Pollution on Photosynthesis Pigment Content in Needles of Picea abies and Picea pungens in Conditions of Development of Iron Ore Deposits
Authors: Fedorchak Elvira 
Published: February 2020

Abstract
We studied photosynthetic pigments in needles of Pісеа аbies and P. pungens in plantings exposed to aerotechnogenic influence of various levels in the big industrial center of steppe zone of Ukraine (Kryvyi Rih). We analyzed the pigment content in needles of the second year of life sampled from 30 to 40-year-old trees of both species in 6 monitoring sites for 5 months. For the needles of P. аbies and P. pungens from all the sites, we noticed the decreasing content of chlorophyll a (to 27.2 and 25.0%, respectively) and chlorophyll b (to 17.9 and 20.0%, respectively) from May till September, in comparison with background territory. At the same time, the content of carotenoids performing the protective function in photosynthetic reactions increased up to 26.1 and 24.0%, respectively. For P. аbies and P. pungens growing in conditions of intensive technogenic pressure, we ascertained that, during investigations, the sum of chlorophylls (a + b) rate decreased to 24.4 and 23.6%, respectively; ratio (a/b) decreased to 11.4 and 12.3%, respectively; ratio (chlorophylls [a + b]/carotenoids) also decreased to 30.1 and 38.0%, respectively, in comparison with plants from the least polluted site. It is shown that the most intensive negative influence on plantings is caused by industrial pollution and exhaust gases: the minimum or, more rarely, the maximum rates of pigment content appeared in needles of the plants exactly from these sites. Our research results demonstrate the feasibility of using the pigment complexes of P. аbies and P. pungens, with the assimilative apparatus sensitive to air pollution damage, as indicators of air environmental conditions.
Changes in Physical and Chemical Properties of Calcic Chernozem Affected by Robinia pseudoacacia and Quercus robur Plantings
Authors: Gorban Vadym , Huslystyi Artem , Kotovych Oleksandr , Yakovenko Volodymyr 
Published: February 2020

Abstract
Growth of forest plantations on soils causes changes in their properties. These changes, their behavior, and magnitude depend on the original soil characteristics and also on the effect of forest plantations being grown. In the steppe zone of Ukraine, Robinia pseudoacacia L. and Quercus robur L. are the woody species most widely used in planting of forest plantations on chernozem soil. Chernozem soil formed exclusively under zonal steppe vegetation and chernozem soil under Robinia pseudoacacia and Quercus robur plantations were studied in this work to analyze the changes in soil properties caused by growth of these tree species. Dry aggregate size distribution, density, particle density, total porosity, organic carbon content, cation exchange capacity, pH values, hydrolytic soil acidity and dry residue, and the available nitrogen, phosphorus, and potassium content were analyzed. The studies found that Robinia pseudoacacia and Quercus robur plantations contribute to an increase in the share of aggregates 2–1 mm in size, as well as formation of aggregate fraction >10 mm, which are completely absent in the Calcic chernozem developed under the steppe vegetation. An increase in the density and particle density, as well as a decrease in the total porosity values were observed under the influence of forest stands studied. This is more common with chernozem under Q. robur plantation. It was found that the carbon percentage decreased in chernozem under the influence of Robinia pseudoacacia growth (on average, 0.4% by a meter-deep layer), but under Quercus robur planting it increased (on average 0.3% by meter-deep layer). Effect of Robinia pseudoacacia plantings on chernozem was also manifested by a decrease in cation exchange capacity (on average, 11 cmol/100 g by a meter-deep layer). The growth of R. pseudoacacia and Quercus robur plantations results in decrease of pH values (0.2 by a meter-deep layer) and increase of hydrolytic soil acidity and dry residue in chernozem water extract. Effect of Robinia pseudoacacia planting leads to a decrease in carbon, nitrogen, and phosphorus content in chernozem. The change in chernozem properties under the influence of Quercus robur plantation is reflected in accumulation of these nutrients. Growth of Robinia pseudoacacia and Quercus robur plantations leads to a decrease in potassium reserves in chernozem, which may indicate its active uptake by these woody species. In general, Q. robur planting is characterized by a large positive effect on the physical and chemical properties of chernozem than Robinia pseudoacacia planting. The findings obtained serve as a ground for making a recommendation for growing Quercus robur plantations under climate conditions of the steppe zone of Ukraine in order to improve the zonal chernozems’ state and fertility.
Modelling Consequences of Maritime Critical Infrastructure Accidents
Authors: Bogalecka Magda 
Published: March 2020

Abstract
The probabilistic general model of critical infrastructure accident consequences including the superposition of three models of the process of initiating events, the process of environment threats and the process of environment degradation is adopted to the maritime transport critical infrastructure. The general model is applied to this critical infrastructure accident with chemical release consequences identification and prediction. The model also includes the cost analysis of losses associated with these chemical releases. Further, under the assumption of the stress of weather influence on the ship operation condition, critical infrastructure accident losses are examined and the results are compared with the previous ones. Finally, the method of optimization are practically tested to the minimizing these losses and the procedures and the new strategy assuring lower environment losses of the considered critical infrastructure accidents are proposed.
Metagenomic analysis of acquired antibiotic resistance determinants in the gut microbiota of wild boars (Sus scrofa) – preliminary results
Authors: Libisch Balázs , Keresztény Tibor , Kerényi Zoltán , Kocsis Róbert , Sipos Rita , Papp Péter P. , Olasz Ferenc 
Published: February 2020

Abstract

Introduction
Land application of manure that contains antibiotics and resistant bacteria may facilitate the establishment of an environmental reservoir of antibiotic-resistant microbes, promoting their dissemination into agricultural and natural habitats. The main objective of this study was to search for acquired antibiotic resistance determinants in the gut microbiota of wild boar populations living in natural habitats.

Material and Methods
Gastrointestinal samples of free-living wild boars were collected in the Zemplén Mountains in Hungary and were characterised by culture-based, metagenomic, and molecular microbiological methods. Bioinformatic analysis of the faecal microbiome of a hunted wild boar from Japan was used for comparative studies. Also, shotgun metagenomic sequencing data of two untreated sewage wastewater samples from North Pest (Hungary) from 2016 were analysed by bioinformatic methods. Minimum spanning tree diagrams for seven-gene MLST profiles of 104 E. coli strains isolated in Europe from wild boars and domestic pigs were generated in Enterobase.

Results
In the ileum of a diarrhoeic boar, a dominant E. coli O112ab:H2 strain with intermediate resistance to gentamicin, tobramycin, and amikacin was identified, displaying sequence type ST388 and harbouring the EAST1 toxin astA gene. Metagenomic analyses of the colon and rectum digesta revealed the presence of the tetQ, tetW, tetO, and mefA antibiotic resistance genes that were also detected in the gut microbiome of four other wild boars from the mountains. Furthermore, the tetQ and cfxA genes were identified in the faecal microbiome of a hunted wild boar from Japan.

Conclusion
The gastrointestinal microbiota of the free-living wild boars examined in this study carried acquired antibiotic resistance determinants that are highly prevalent among domestic livestock populations.
Association Between 4q25 Variants, Risk of Atrial Fibrillation and Echocardiographic Parameters
Authors: Rudaka Irina , Rots Dmitrijs , Uzars Arturs , Kalējs Oskars , Gailīte Linda 
Published: February 2020

Abstract
Atrial fibrillation (AF), the most common type of arrhythmia, has a heritable component. Variants at locus 4q25 are best associated with the risk of AF development in genome-wide association studies. Left atrial volume is an independent predictor of recurrence of AF after successful sinus rhythm restoration. The aim of our study was to investigate potential association between genetic variants at 4q25 locus and the risk of AF and echocardiographic parameters. We included 241 AF patients and 119 control individuals into the study. Left ventricle ejection fraction (LVEF, %) and left atrial volume index (LAVI, ml/m2) were assessed by transthoracic echocardiography during outpatient visits. We selected five 4q25 genetic variants (rs6825911, rs1126483, rs10004516, rs6838973, rs2200733) for the analysis. Variant rs6838973 was found to be associated with reduced risk of AF in additive (CCTT) and dominant (CC vs. CT+TT) models of inheritance. On the other hand, additive (CC<CT<TT) and dominant (CC vs. CT+TT) models of rs2200733 were associated with greater risk of AF. The same variations were found to be associated with age of AF onset. Median LAVI was 39.0 ml/m2 (IQR = 10.0) and median LVEF was 56.0% (IQR = 13.0). Statistically significant association was observed only between LAVI and variant rs1126483 in the dominant model of inheritance (median LAVI in CC vs. CT+TT – 38 ml/m2 vs. 40 ml/m2, U = 1602.5, p = 0.032). No significant association was found for LVEF and the analysed genotypes.
Risk Factor Analysis for Gout in the Latvian Population
Authors: Nagle Erika , Luksa Nellija , Moisejevs Georgijs , Gailīte Linda 
Published: February 2020

Abstract
Gout (Gr. podagra) is a multifactorial pathology, which means that both genetic and environmental factors play a role in the aetiology of the disease. For these reasons, revealing risk factors could be of very high importance in prevention and treatment of gout. In the present study, we found that a major role in gout predisposition is played by specific alleles in the ABCG2 gene. The study used survey data for 43 gout patients and 99 healthy control individuals who were geno-typed for rs2231142. It was found that allelic variant rs2231142 in the ABCG2 gene had the strongest association with gout. Among other co-factors studied, sex, and increased body mass index were associated with gout.
Cultivation of 3D Dermal Tissue by Application of Autologous Matrix
Authors: Jakobsons Ēriks , Ērglis Kristaps , Ramata-Stunda Anna , Blāķe Ilze , Patetko Liene , Tīcmane Simona , Rupaine Beatrise Lūcija , Ērglis Mārtiņš , Ērgle Māra , Strīķe Eva , Strazdiņš Uldis , Rubīns Silvesters , Rubīns Andris , Ērglis Andrejs 
Published: February 2020

Abstract
The most common reasons for major skin loss are thermal trauma — burns and scalds that can result in rapid, extensive, deep wounds as well as chronic non-healing wounds. Treatment using common techniques is poor and depending on the trauma level can result in death. There is a substantial need for skin integrity restoration. The main goal of this study was to develop an autologous 3D skin model that could eventually be translated into clinical applications. The study examined a variety of factors — extracellular matrix components, cell count, culture medium modification and role of structurally and functionally high-quality 3D skin dermis layer tissue culture production. The results of this study are an essential prerequisite to standardise the use of both clinical, as well as in vitro test systems. Dermal cell lines applied in the study were isolated form patient biopsies obtained at Pauls Stradiņš Clinical University Hospital. Blood plasma type AB was used for fibrin matrix formation. As catalysts, CaCl2 or calcium gluconate, and tranexamic acid were applied. 3D tissue functionality was assessed by evaluation of gene expression and changes in growth factor secretion. Fibrin matrix formulations with 1% and 1.5% CaCl2 and 5 mg, 7 mg and 10 mg tranexamic acid concentration were tested. Better matrix properties were observed with higher concentration of CaCl2 and tranexamic acid. Differences in levels of collagen gene expression and growth factor secretion were observed. Changes in levels of fibroblast growth factor and gene expression were observed in fibrin matrix samples and the surface-cultivated cell culture monolayer, but structural protein synthesis was not detected.
Perioperative radiotherapy versus surgery alone for retroperitoneal sarcomas: a systematic review and meta-analysis
Authors: Diamantis Alexandros , Baloyiannis Ioannis , Magouliotis Dimitrios E. , Tolia Maria , Symeonidis Dimitrios , Bompou Effrosyni , Polymeneas Georgios , Tepetes Konstantinos 
Published: March 2020

Abstract

Background
There is no clear evidence on whether radiotherapy (RT) improves treatment result in patients with retroperitoneal sarcomas (RPS).

Methods
A systematic literature search was performed using PubMed, Scopus and CENTRAL databases. Data were retrieved from published comparatives studies in patients with RPS undergoing surgery alone or RT plus surgery. The primary endpoints were the 5-year OS and the median OS. The secondary endpoints were the recurrence-free survival (RFS) and the R0-resection rate. Continuous outcomes were calculated by means of weighted mean difference (WMD).

Results
Ten out of 374 articles were analyzed. The median OS and the 5-year survival were significantly increased in patients treated with RT and surgery, compared to patients treated with surgery alone (p < 0.00001, p < 0.001). Median RFS was significantly increased in patients treated with either preoperative (p < 0.001) or postoperative (p = 0.001) RT compared to patients that underwent surgery alone. Finally, median R0-resection rate was similar between the two groups (p = 0.56).

Conclusion
RT along with radical surgery could be the standard of care in at least a subgroup of patients with RPS.
Kesembuhan Lesi Dermatitis Kompleks dengan Pemakaian Krim Herbal
Authors: Junaedi Putu Adrian , Suartha I Nyoman , Sudimartini Luh Made 
Published: February 2020

Dermatitis merupakan istilah peradangan yang terjadi pada kulit, akibat dari infeksi mikroorganisme dan faktor genetik, ketahanan kulit dan metabolisme. Tujuan dari penelitian ini adalah untuk mengetahui kesembuhan lesi pada anjing yang menderita dermatitis kompleks yang diberi sediaan krim herbal. Krim herbal yang digunakan berasal dari ekstrak tanaman nimba, pegagan, dan sirsak. Penelitian ini menggunakan 4 ekor anjing yang dibagi menjadi 2 kelompok. Kelompok A adalah 3 ekor anjing yang diberi sediaan krim herbal selama 15 hari dan Kelompok B adalah 1 ekor anjing sebagai kontrol tanpa pemberian krim herbal. Pengamatan kesembuhan lesi dilakukan dimulai dari hari ke-0 sampai hari ke-15. Data yang didapatkan diuji dengan uji Wilcoxon dan dianalisa secara deskriptif. Hasil penelitian menunjukkan pada kelompok A yang diberi krim herbal mengalami tingkat kesembuhan lesi lebih cepat dibandingkan kelompok B yang tanpa pemberian krim herbal. Pemberian krim herbal berpengaruh nyata (P?0,05) terhadap anjing yang menderita dermatitis kompleks. Kesimpulan pemberian krim herbal dapat mempercepat kesembuhan lesi pada anjing yang menderita dermatitis kompleks.
Aktivitas Alanin Aminotransferase dan Aspartat Aminotransferase pada Broiler yang Diberikan Penambahan Tepung Temulawak dalam Pakan
Authors: Puspa Adhi Putu Diah , Ardana Ida Bagus Komang , Kendran Anak Agung Sagung 
Published: March 2020

Temulawak (Curcuma xanthorrhiza Roxb.) merupakan salah satu tanaman obat yang banyak digunakan oleh masyarakat, termasuk digunakan secara luas pada hewan karena mempunyai banyak khasiat bagi kesehatan. Tujuan penelitian adalah untuk mengetahui efek penambahan tepung temulawak dalam pakan terhadap aktivitas ALT dan AST pada broiler. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 24 ekor broiler betina umur 14 hari yang dibagi menjadi 4 kelompok, yaitu kelompok P0 (pakan normal tanpa penambahan tepung temulawak), kelompok P1 (tepung temulawak 10 gr/kg pakan), P2 (tepung temulawak 20 gr/kg pakan), dan P3 (tepung temulawak 30 gr/kg pakan). Setelah umur 35 hari darah diambil melalui vena brachialis menggunakan spuit 5 ml selanjutnya dilakukan pemeriksaan aktivitas ALT dan AST dengan menggunakan Photometer 5010. Data dianalisis dengan menggunakan Sidik Ragam/ANOVA. Hasil penelitian menunjukkan bahwa penambahan tepung temulawak dalam pakan broiler tidak mempengaruhi aktivitas ALT dan AST (P>0,05). Dari hasil penelitian dapat disimpulkan bahwa penambahan tepung temulawak dalam pakan broiler dosis 10 gr/kg pakan sampai 30 gr/kg pakan selama 21 hari masih tergolong dosis aman.
Narcissitic Personality Disorder Pada Tokoh Elena Dalam Komik Tomodachi Gokko Karya Momochi Reiko
Authors: Wardhani Komang Triyas , Damayanti Silvia , Anggraeny Renny 
Published: February 2020

The tittle of this research “Narcissitic Personality Disorder at Character Elena Figure in comic Tomodachi Gokko by Momochi Reiko”. The aims of this research are to described NPD Characteristic, the reasons of NPD in character Elena, impact of NPD to Elena and other characters in comic Tomodachi Gokko by Momochi Reiko.The theories that have been used are Wellek and Warren’s literature and psychology theory (2016), Sigmund Freud Narcissism theory (1914), Gunderson’s Narcissitic Personality Disorder (1994), Lajos Egri’s Three Dimensions of Characterrization (1946) and Marcel Danesi’s semiotic theory (2011).
Based on the result, Elena is an early adulthood’s girl who experiences with Narcissistic Personality Disorder. Characteristic of NPD in Elena based on Gunderson, dkk, namely (1) has a grandiose of self-importance and is often envious of others, (2)is preoccupied with fantasies of unlimited success, power, brilliance, beauty, or ideal love. (3) believes that he or she is “special” and unique and can only be understood by, or should associate with, other special or high status people, (4) requires excessive admiration, (5) has a sense of entitlement, (6) is interpersonally exploitative, takes advantage of other to achieve his or her own ends, (7) lacks empathy, and (8) shows arrogant, haughty behaviors or attitudes. The Reason behind Elena’s NPD condition was triggered by her mother, as a child she was often got curse word. The impact of NPD to Elena were 1) difficulted for her to distinguish between good and bad deeds and she almost lost her own life, and she move to the new school. The impact Elena’s NPD to other people around her were 2) she doing bullying, damaging, and traping for personal gain.
Keywords : psychology literature, Narcissistic Personality Disorders, Psychoanalytic
Ekologi dan Potensi Invasif Acacia decurrens di Sebagian Kawasan Taman Nasional Gunung Merapi Yogyakarta
Authors: Sutomo Sutomo 
Published: February 2020

Acacia decurrens, originally from Australia, is a woody plant that has become a concern in the erupted areas of Merapi volcano in 2006. The purpose of this study was to quantitatively describe the ecology of Acacia decurrens, and its invasive potential. Vegetation analysis was carried out in four areas of Mount Merapi National Park (TNGM), namely Kalikuning, Kaliadem, Plawangan and Pranajiwa. Ordination using the Non Metric Multidimensional Scaling (NMDS) method and Canonical Correspondence (CCA) and Spearman's bivariate correlation were carried out in data analysis. The results of NMDS analysis (2D stress = 0.14) showed that open areas due to eruption in Kaliadem are now dominated by A. decurrens. The results of the analysis also showed a significant negative correlation (Spearman rho = 0.6) between the abundance of A. decurrens and the level of species diversity in the sampling location. From the results of CCA, A. decurrens in the seedling stage appeared to coexist with other types of ground cover such as Alangium javanicum and Araliaceae. But in the tree phase, this species tended to form pure stands and only occasionally appeared to live side by side with other types of Fabaceae such as Albizia lopantha. Tree-level A. decurrens seemed to prefer sites with lower pH levels while A. decurrens seedling levels were more commonly found in sites with higher pH. Acacia decurrens have the potential to become a weed in TNGM due to its dominant distribution and tendency to decrease the level of species diversity in TNGM.
Sperm Quality Reduction of Rats (Rattus novergivus) Injected by High Dosage of White Vitamin C in Long Term
Authors: Sudatri Ni Wayan , Yulihastuti Dwi Ariani , Suartini Ni Made 
Published: February 2020

Benefits of white vitamin C to increace the immune system is already exis. However, the use of high doses of vitamin C and in the long term can cause side effects.. The objective of this study was to determine the side effects of high-dose injections of vitamin C in the long term on sperm quality of rats. The design used in this study is completely randomized design (CRD) with long treatment injections of high doses of vitamin C are different. ie: P0 (control), P1 (injected 30 days), P2 (injected 50 days ), P3 (injected 70 days) and P4 (injected 90 days). Parameters measured were sperm quality (motility, viability, morphology, number) ,. The results showed that the sperm quality of rats (Rattus novergivus). Sperm quality of male rats (Rattus norvegivus L.) were injected vitamin C in high doses for long periods of time between the control and treatment groups showed significant differences (P = 0.000). The quality of sperm of mice injected high doses of white vitamin C tend to decrease sperm quality indicated by percentage of motility, viability, and morphology of spermatozoa although the concentration of spermatozoa tends to increase.
Keywords: white vitamin C, rat, sperm quality
Efek Toksik Minyak Atsiri Limbah Daun Kayu Manis (Cinnamomum burmanii (Nees & T.Nees) Blume.) dalam Mengendalikan Helopeltis antonii Signoret pada Tanaman Kakao secara in Vitro
Authors: Puspita Roza , Rahayu Resti , Mairawita Mairawita , Nasir Nasril , Nurmansyah Nurmansyah 
Published: February 2020

The toxic effect of essential oil from waste cinnamon leaf (Cinnamomum burmanii) against pests of cocoa fruit Helopeltis antonii (Hemiptera; Miridae), has been done in Green House, Research Station Spice and Medicinal Plants, Laing Solok, West Sumatra in April to May 2016. The experiment aimed to determine repellant and lethal concentrations of essential oil from waste s cinnamon leaf (C. burmanii) for H. antonii. There were 5 treatments arranged in Complately Randomized Design with 4 replications. The treatments tested were essentialoil concentration f (0.05 ml/tube, 0.1 ml/tube, 0.2 ml/tube and 0.5 ml/tube) and control. The results showed that the effective repellent concentration were 0,05 ml/tube and 0,1 ml/tube which repelled H. antonii 0,03 % . The effecive lethal concentration was 0,05 ml/tube which killed H. antonii 57,50 %. Concentration used was highly toxic which killed l H. Antonii within six hours so of the repellent nature was notobserved.