bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluorescence‐based quantitative platform for ultrasensitive food allergen detection: From immunoassays to DNA sensors
Authors: Fu Linglin , Qian Yifan , Zhou Jinru , Zheng Lei , Wang Yanbo 
Published: October 2020

AbstractFood allergies are global health issue with an increasing prevalence that affect food safety; hence, food allergen detection, labeling, and management are considered to be important priorities in the food industry. In this critical review, we provide a comprehensive overview of several fluorescence‐based platforms based on different biorecognition ligands, such as antibodies, DNA, aptamers, and cells, for food allergen quantification. Traditional analytical methods are generally unsuitable for food manufacturers to accomplish the real‐time identification of food allergens in food products. Therefore, it is important to develop simple, rapid, inexpensive, accurate, and sensitive methods to improve user accessibility. A fluorescence‐based quantitative platform provides an excellent detection platform for food allergens because of its high sensitivity. This review summarizes the traditional antibody‐based fluorescent techniques for food allergen detection, such as the time‐resolved fluoroimmunoassay , immunofluorescence imaging, fluorescence enzyme‐linked immune sorbent assay, flow injection fluoroimmunoassay, and fluorescence immunosensors. However, these methods suffer from disadvantages such as the significant rate of false‐positive and false‐negative results due to antibody cross‐reactivity with nontarget food components in the complex food matrix and epitope degradation during food processing. Hence, different types of fluorescence‐based immunoassays are suitable for standardization and quantification of allergens in fresh foods. In addition, we summarize new fluorescence‐based quantitative platforms, including fluorescence genosensors, fluorescence cell sensors, and fluorescence aptamer sensors. With the advantages of high sensitivity and simple operation, fluorescence biosensors will have great potential in the future and could provide portable methods for multiallergen real‐time detection in complex food systems.
Food safety in Peru: A review of fresh produce production and challenges in the public health system
Authors: Ramirez‐Hernandez Alejandra , Galagarza Oscar A. , Álvarez Rodriguez Mariel V. , Pachari Vera Erika , Valdez Ortiz María del Carmen , Deering Amanda J. , Oliver Haley F. 
Published: October 2020

AbstractPeru has a commodities‐based economy where agriculture plays an essential role in the nation's development. Among agricultural products, fruits and vegetables are foundational to Peruvian culture and a healthy and nutritious diet. Produce is also the primary income source for thousands of small‐scale farmers and producers throughout the country. Peru has significant potential to export agricultural and value‐added products. Nevertheless, the Peruvian food chain has weak food safety and quality standards, limiting access to international markets. The inherent lack of food safety surveillance and management systems negatively affects public health. In the past decade, fresh and raw produce has been associated with several foodborne outbreaks worldwide, resulting in significant health and economic losses. This alarming situation for public health officials and regulators has called for the strengthening of produce safety standards and food safety risk management for safer food and to reduce the incidence of foodborne illnesses. This review summarizes the current status of produce safety in Peru and explores opportunities (e.g., policy, university capacity development) toward a safer food system.
Toad‐kill: Prey diversity and preference of invasive guttural toads (Sclerophrys gutturalis) in Mauritius
Authors: Baxter‐Gilbert James , Florens F. B. Vincent , Baider Cláudia , Perianen Yuvna Devi , Citta Denzel Shane , Appadoo Chandani , Measey John 
Published: October 2020

AbstractThe invertebrate communities of Mauritius host a high degree of endemism, but are also imperilled by an array of factors, including invasive predators. Since their introduction in 1922, guttural toads (Sclerophrys gutturalis) have spread across the island and have been implicated in the decline of a number of endemic invertebrate species. In this study, we examined the feeding habits of the invasive population of guttural toads from three naturally forested locations in Mauritius across multiple years by analysing their stomach content. We also measured the relative abundance of prey items on the landscape using pitfall traps and applied these data to determine prey preference using a Relativised Electivity Index. Insects, malacostracans and gastropods constituted the bulk of the toads' diet (48.7%, 33.4% and 11.8%, respectively), which also included several rare and endemic species. We further determined that insects and malacostracans were also the two most favoured prey taxa, relative to what was available on the landscape. Our investigation has generated several recommendations for future research and provides a fundamental understanding of the diet of guttural toads in the native forests of Mauritius.
Changes in use of hormonal long‐acting reversible contraceptive methods in Australia between 2006 and 2018: A population‐based study
Authors: Grzeskowiak Luke E. , Calabretto Helen , Amos Natalie , Mazza Danielle , Ilomaki Jenni 
Published: October 2020

BackgroundLong‐acting reversible contraceptives (LARCs) are promoted internationally as a key strategy for reducing unintended pregnancy and abortion rates.AimsTo examine trends in use of hormonal LARCs among reproductive‐aged women in Australia between 2006 and 2018 and explore trends according to age groups and state/territory of dispensing.Materials and MethodsRetrospective population‐based study using Pharmaceutical Benefits Scheme (PBS) dispensing claims of a 10% random sample of females aged 15–44. We investigated rates and annual trends in dispensing claims of etonorgestrel implant and levonorgestrel intrauterine systems (IUS).ResultsBetween 2006 and 2018, annual PBS claims for LARCs increased approximately two‐fold from 21.7 to 41.5 per 1000 women, with a plateau observed from 2015 onward. Absolute rate increases were similar for the implant (9.0/1000) and IUS (10.8/1000), with increases observed across all age groups and states/territories. Overall dispensing rates varied by two‐fold according to state/territory of dispensing and four‐fold according to age groups. Rate increases for the implant were highest among the 15–19 and 20–24 age groups, while rate increases for the IUS were highest among the 35–39 and 40–44 age groups. It is estimated that in 2018, 10.8% of women aged 15–44 were using a LARC; 4.5% for the implant and 6.3% for the IUS.ConclusionsRates of hormonal LARC use have doubled over the past decade. Investigating underlying reasons for the large observed differences in rates of use according to age and state/territory could help further improve uptake for these most effective methods of contraception.
Surgical management of super‐super obesity with grade III esophageal varices and liver cirrhosis: The ultimate challenge
Authors: Bhambri Amit , Singla Vitish , Kaul Aashir , Shalimar  , Kashyap Lokesh , Aggarwal Sandeep 
Published: October 2020

AbstractThe risk of complications after bariatric surgery is high in morbidly obese patients suffering from liver cirrhosis along with moderate to severe portal hypertension. Esophageal varices are even considered as a contraindication for bariatric surgery by many surgeons. We report the case of a 40‐year‐old gentleman with a body mass index of 65.3 kg/m2, with multiple comorbidities including type 2 diabetes mellitus, severe obstructive sleep apnea. On evaluation, he had Child‐Pugh A liver cirrhosis with portal hypertension along with grade III esophageal varices and splenomegaly. After adequate optimization, laparoscopic sleeve gastrectomy was performed. The patient is doing well at a follow up of 12 months with an adequate weight loss and resolution of comorbidities. Sleeve gastrectomy can be performed in a morbidly obese Child‐Pugh A cirrhotic patient with portal hypertension and esophageal varices with proper counseling regarding more than usual risk for morbidity and mortality.
Predictors for failure of endoscopic ureteric stenting in patients with malignant ureteric obstruction: systematic review and meta‐analysis
Authors: Guachetá‐Bomba Pedro Luis , Echeverría‐García Fernando , García‐Perdomo Herney Andrés 
Published: October 2020

ObjectiveTo determine the factors for failure of endoscopic ureteric stenting in patients with malignant ureteric obstruction.MethodsWe performed a search strategy in the Medical Literature Analysis and Retrieval System Online (MEDLINE), the Excerpta Medica dataBASE (EMBASE), the Literatura Latino‐Americana e do Caribe em Ciências da Saúde database (LILACS), and the Cochrane Central Register of Controlled Trials (CENTRAL) databases. We included patients with malignant ureteric obstruction, who had a JJ catheter insertion. The studies reported the percentage of failure and risk factors, e.g. bladder invasion or deformity of the trigone, hydronephrosis, renal failure, previous radiotherapy, age, obstruction aetiology, and patient’s health status. We performed a meta‐analysis using R software (‘meta’ and ‘metafor’ libraries).ResultsWe included nine studies that met the inclusion criteria, with 761 patients and an average age of 60.5 years. The studies assessed the time to failure during the first 30 days. The reported failure rate was 32% (95% confidence interval [CI] 21–45%; I2 = 88%). Regarding risk factors for failure, bladder invasion or deformity of the trigone had a hazard ratio (HR) of 4.8 (95% CI 1.28–8.5; I2 = 97.4%); severe hydronephrosis had a HR of 3.92 (95% CI 0.32–7.52; I2 = 93.9%); and age <65 years had a HR of 0.93 (95% CI 0.8–0.9; I2 = 0%).ConclusionsWe found a high probability of failure for endoscopic urinary decompression in patients with malignant ureteric obstruction. Factors such as bladder invasion or deformity of the trigone and age >65 years had an increased risk of failure.
Cytokine activin C ameliorates chronic neuropathic pain in peripheral nerve injury rodents by modulating the TRPV1 channel
Authors: Huang Ya‐Kun , Lu Yu‐Gang , Zhao Xin , Zhang Jing‐Bing , Zhang Feng‐Ming , Chen Yong , Bi Ling‐Bo , Gu Jia‐Hui , Jiang Zuo‐Jie , Wu Xiao‐Man , Li Qing‐Yi , Liu Yanli , Shen Jian‐Xin , Liu Xing‐Jun 
Published: October 2020

Background and PurposeThe cytokine activin C is mainly expressed in small‐diameter dorsal root ganglion (DRG) neurons and suppresses inflammatory pain. However, the effects of activin C in neuropathic pain remain elusive.Experimental ApproachMale rats and wild‐type and TRPV1 knockout mice with peripheral nerve injury ‐ sciatic nerve axotomy and spinal nerve ligation in rats; chronic constriction injury (CCI) in mice – provided models of chronic neuropathic pain. Ipsilateral lumbar (L)4–5 DRGs were assayed for activin C expression. Chronic neuropathic pain animals were treated with intrathecal or locally pre‐administered activin C or the vehicle. Nociceptive behaviours and pain‐related markers in L4–5 DRGs and spinal cord were evaluated. TRPV1 channel modulation by activin C was measured.Key ResultsFollowing peripheral nerve injury, expression of activin βC subunit mRNA and activin C protein was markedly up‐regulated in L4–5 DRGs of animals with axotomy, SNL or CCI. [Correction added on 26 November 2020, after first online publication: The preceding sentence has been corrected in this current version.] Intrathecal activin C dose‐dependently inhibited neuropathic pain in spinal nerve ligated rats. Local pre‐administration of activin C decreased neuropathic pain, macrophage infiltration into ipsilateral L4–5 DRGs and microglial reaction in L4–5 spinal cords of mice with CCI. In rat DRG neurons, activin C enhanced capsaicin‐induced TRPV1 currents. Pre‐treatment with activin C reduced capsaicin‐evoked acute hyperalgesia and normalized capsaicin‐evoked persistent hypothermia in mice. Finally, the analgesic effect of activin C was abolished in TRPV1 knockout mice with CCI.Conclusion and ImplicationsActivin C inhibits neuropathic pain by modulating TRPV1 channels, revealing potential analgesic applications in chronic neuropathic pain therapy.
Maintenance and risk factors for fractures of overdentures using immediately loaded conventional diameter or mini implants with Locator abutments: A cohort study
Authors: Mifsud David P. , Cortes Arthur R. G. , Zarb Mario J. , Attard Nikolai J. 
Published: October 2020

AbstractBackgroundFractures in mandibular implant‐retained overdentures are a common complication. However, little is known on the related risk factors and outcome differences when using two conventional diameter or two mini implants.PurposeThe purpose of this study was first, to evaluate the required maintenance and complications with the overdentures and second, to analyze risk factors for overdenture fractures.Materials and MethodsThis cohort study was conducted in edentulous patients with complete dentures. Patients received either two conventional (4.1 mm in diameter) and two mini (2.9 mm or less in diameter) implants, based on available buccal‐lingual ridge width. All implants were immediately loaded with mandibular overdentures retained by Locator abutments. The number of prosthodontic after care visits (scheduled and unscheduled) were recorded and compared between the two implant diameter groups. Fracture occurrence was the primary outcome variable. Risk estimates were presented as odds ratios (ORs) with 95% confidence intervals (CIs). The ORs were adjusted for potential clinical confounders (ie, necessity of relining, matrix recapture, abutment loosening, implant diameter, height of the Locator, and retention force).ResultsA total of 50 edentulous patients were analyzed. One conventional diameter and three mini implants failed within a 1‐year follow‐up period. Prosthetic maintenance requirements for overdentures on both implant diameter groups were comparable. A total of 12 overdenture fractures (four in the mini implant group and eight in the conventional diameter group) occurred. Adjusted OR (AOR) analysis showed a significant association between abutment loosening and overdenture fracture (AOR = 12.00, 95% CI = 1.11‐129.45; P = .041).ConclusionWithin the limitations of this study, the present findings suggest that implant diameter does not affect number of prosthetic maintenance and complications, and that abutment loosening is a risk factor for overdenture fractures, regardless of the implant diameter used.
Integration of recurrent convolutional neural network and optimal encryption scheme for intrusion detection with secure data storage in the cloud
Authors: Prabhakaran Varun , Kulandasamy Ashokkumar 
Published: October 2020

AbstractData communication security is growing day after day with the proliferation of cloud computing. It is primarily because of the few security constraints and challenges occurring in the cloud environment during data transmission. Existing research has shown that the intrusion detection system (IDS) centered on the cloud is more complicated. In this article, we address the above issues by proposing an attention‐based recurrent convolutional neural network (RCNN). This proposed RCNN is used to detect whether the text data are intrusion or nonintrusion. The nonintrusion text information is then used for further processing and encrypted using a two‐way encryption scheme. We introduce the elliptical curve cryptography (ECC) approach to increase the security‐level performance of nonintrusion data. Moreover, the integration of ECC with the modified flower pollination algorithm (MFP‐ECC) creates the two‐way encryption scheme, and it is used to produce an optimal private key. The encrypted data are then stored in a cloud environment by steganography and the data with the sensitive information are replaced by some other text, thus providing security to the data at rest. The proposed MFP‐ECC approach shows maximum breaking time results and can also withstand different classical attacks when compared with other methods. As a result, the proposed intrusion detection and secure data storage mechanism is highly secured and it is never affected by any kinds of conspiracy attacks.
Antenatal diagnosis of fetal intraventricular hemorrhage: systematic review and meta‐analysis
Authors: Dunbar Mary J , Woodward Kristine , Leijser Lara M , Kirton Adam 
Published: October 2020

AimTo determine how the severity of antenatally diagnosed germinal matrix‐intraventricular hemorrhage (GMH‐IVH) relates to morbidity and mortality, and to explore potential risk factors.MethodWe conducted a systematic review and individual patient data meta‐analysis of antenatally diagnosed fetal GMH‐IVH. The primary outcomes were mortality and morbidity. Potential associations with clinical factors during pregnancy were explored. Analysis employed Fisher’s exact test and logistic regression.ResultsWe included 240 cases from 80 studies. Presence of venous infarction was associated with mortality (odds ratio [OR] 4.3, 95% confidence interval [CI] 1.4–13.25), motor impairment (OR 103.2, 95% CI 8.6–1238), epilepsy (OR 6.46, 95% CI 2.64–16.06), and developmental delay (OR 8.55, 95% CI 2.12–48.79). Shunt placement was associated with gestational age at GMH‐IVH diagnosis and in utero progression. Many cases had uncomplicated pregnancies but possible co‐occurring conditions included twin gestation, small for gestational age, and congenital anomalies.InterpretationSeverity of fetal GMH‐IVH, specifically venous infarction, is associated with overall mortality and morbidity. Risk factors for fetal GMH‐IVH are poorly understood and controlled studies are required.What this paper adds

Preterm germinal matrix‐intraventricular hemorrhage (GMH‐IVH) grading can be applied to fetuses.

Many fetal germinal matrix hemorrhages occur in otherwise typical pregnancies.

Half of fetuses with post‐hemorrhagic ventricular dilatation receive a shunt after delivery.

Fetuses with grade I or II GMH‐IVH have few sequelae.

Fetuses with periventricular hemorrhagic infarction have a high burden of motor impairment.
Acquired peri‐articular ganglion cyst in the lateral femorotibial joint in an 18‐year‐old Percheron cross mare
Authors: Alsherif A. A. , Myhre G. D. , Vin R. 
Published: October 2020

SummaryThis report describes a case of sudden lameness in an 18‐year‐old Percheron cross mare after presumably being kicked by another horse, and the subsequent formation of a ganglion cyst originating from the lateral femorotibial joint. Physical examination, radiographic and ultrasonographic investigation identified a soft round 5 cm diameter mass attached to the left lateral femorotibial joint. After surgical removal, histopathological examination confirmed a ganglion cyst. The horse responded well to the surgical removal of the cyst, and 4 weeks after the surgery, the mare has returned back to her same athletic performance. According to the authors’ knowledge, this is the first reported case that describes a peri‐articular ganglion cyst originating from the stifle joint.
Buffering effects of soil seed banks on plant community composition in response to land use and climate
Authors: Plue Jan , Van Calster Hans , Auestad Inger , Basto Sofía , Bekker Renée M. , Bruun Hans Henrik , Chevalier Richard , Decocq Guillaume , Grandin Ulf , Hermy Martin , Jacquemyn Hans , Jakobsson Anna , Jankowska‐Błaszczuk Małgorzata , Kalamees Rein , Koch Marcus A. , Marrs Rob H. , Marteinsdóttir Bryndís , Milberg Per , Måren Inger E. , Pakeman Robin J. , Phoenix Gareth K. , Thompson Ken , Vandvik Vigdis , Wagner Markus , Auffret Alistair G. 
Published: October 2020

AbstractAimClimate and land use are key determinants of biodiversity, with past and ongoing changes posing serious threats to global ecosystems. Unlike most other organism groups, plant species can possess dormant life‐history stages such as soil seed banks, which may help plant communities to resist or at least postpone the detrimental impact of global changes. This study investigates the potential for soil seed banks to achieve this.LocationEurope.Time period1978–2014.Major taxa studiedFlowering plants.MethodsUsing a space‐for‐time/warming approach, we study plant species richness and composition in the herb layer and the soil seed bank in 2,796 community plots from 54 datasets in managed grasslands, forests and intermediate, successional habitats across a climate gradient.ResultsSoil seed banks held more species than the herb layer, being compositionally similar across habitats. Species richness was lower in forests and successional habitats compared to grasslands, with annual temperature range more important than mean annual temperature for determining richness. Climate and land‐use effects were generally less pronounced when plant community richness included seed bank species richness, while there was no clear effect of land use and climate on compositional similarity between the seed bank and the herb layer.Main conclusionsHigh seed bank diversity and compositional similarity between the herb layer and seed bank plant communities may provide a potentially important functional buffer against the impact of ongoing environmental changes on plant communities. This capacity could, however, be threatened by climate warming. Dormant life‐history stages can therefore be important sources of diversity in changing environments, potentially underpinning already observed time‐lags in plant community responses to global change. However, as soil seed banks themselves appear, albeit less, vulnerable to the same changes, their potential to buffer change can only be temporary, and major community shifts may still be expected.
Establishing hematological reference intervals in healthy adults: Ravansar non‐communicable disease cohort study, Iran
Authors: Moradinazar Mehdi , Najafi Farid , Pasdar Yahya , Hamzeh Behrooz , Shakiba Ebrahim , Bohn Mary Kathryn , Adeli Khosrow , Rahimi Zohreh 
Published: October 2020

AbstractIntroductionIt is necessary to establish hematological reference intervals (RIs) in each population to improve disease management and healthcare quality. This study aimed to establish age‐ and sex‐specific hematological RIs in a healthy adult Kurdish population and evaluate the influence of select lifestyle factors.MethodsHematological parameters were studied in 6518 individuals (3006 females, 3512 males) from Ravansar Non‐Communicable Disease (RaNCD) cohort study. Hematological parameters were measured by Beckman Coulter HmX Analyzer. After combined application of exclusion criteria and statistical outlier removal, RIs for all partitions were calculated using nonparametric methods.ResultsThe present study established hematological RIs for 14 parameters in a healthy adult Iranian population. Reference values for some analytes demonstrated significant age‐ and sex‐specific differences and were slightly different when compared to RIs determined in other populations. Furthermore, the current smokers had higher levels of white blood cells (WBCs), red blood cells (RBCs), hemoglobin, hematocrit, mean corpuscular hemoglobin (MCH), and mean corpuscular volume than ex‐ and nonsmokers. Also, in the presence of high physical activity, elevated levels of RBC, hemoglobin, hematocrit, monocytes, and MCH were observed, as well as lower WBC levels. Further, a significant positive association was observed between body mass index (BMI) and WBC, red cell distribution width, and plateletcrit levels.ConclusionOur study suggests hematological parameters are influenced by age, sex, and lifestyle factors such as physical activity and BMI. Additionally, discrepancies when compared to other population studies suggest that ethnic‐specific differences need to be considered when establishing RIs for hematological parameters.