bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glomulina oculus, New Calcareous Foraminiferal Species from the High Arctic: A Potential Indicator of a Nearby Marine-Terminating Glacier
Authors: Jennings Anne E. , Seidenkrantz Marit-Solveig , Knudsen Karen Luise 
Published: July 2020

ABSTRACT
A new calcareous Arctic foraminiferal species, Glomulina oculus n. sp., belonging to the suborder Miliolina has been observed in surface samples from northern Nares Strait and Petermann Fjord, NW Greenland, and off Zachariae Isbrae, NE Greenland, as well as in early Holocene sediments from the northern Baffin Bay region and on the NE Greenland shelf. In some samples, this new porcelaneous species makes up a significant fraction of the foraminiferal assemblage, particularly in samples with a relatively large sand content, and we suggest that this species is indicative of an Arctic environment with marine-terminating glaciers. Yet, further studies are needed to ascertain its full habitat range.
Association between levels of tumor-infiltrating lymphocytes in different subtypes of primary breast tumors and prognostic outcomes: A meta-analysis
Authors: He Lin , Wang Yaling , Wu Qian , Song Yuhua , Ma Xuezhen , Zhang Biyuan , Wang Haiji , Huang Yong 
Published: July 2020

Abstract
Background: To investigate the impact of the elevation of tumor-infiltrating lymphocytes (TILs) in different molecular subtypes of primary breast cancer, i.e. each 10% increment of TILs and high-level TILs (TILs≥50%) in tumor, on overall survival (OS) and pathological complete response (pCR) and to compare the presentation of high-level TILs across these molecular subtypes. Methods: Citation retrieval was performed in the PubMed, Cochrane Library, Embase and Web of Science databases. All statistical calculations were performed by the software of StataSE version 12.0. Results: Twenty-two eligible clinical trials including 15676 unique patients were included for meta-analysis. Each 10% increment of TILs significantly improved OS in human epidermal growth factor receptor 2 (HER2)-overexpression (pooled Hazard ratio (HR), 0.92; 95% CI, 0.89-0.95) and triple-negative (TN) (pooled HR, 0.90; 95% CI, 0.89-0.92) breast tumors but not in luminal tumor subtype (pooled HR, 1.06; 95% CI, 0.99-1.13). It was also associated with an increased pCR rate in breast cancers (pooled Odds ratio (OR), 1.27; 95% CI, 1.19-13.5). High-level TILs were significantly related with a higher pCR rate (pooled OR, 2.73; 95% CI, 2.40-3.01) than low-level TILs. The HER2-amplified (pooled OR, 3.14; 95% CI, 1.95-5.06) and TN (pooled OR, 4.09; 95% CI, 2.71-6.19) phenotypes of breast cancers expressed significantly more high-level TILs than the luminal tumor subtype, although the presentation of those between the former two subsets was not significantly different (pooled OR, 1.30; 95%CI, 0.83-2.04). Conclusions: The elevation of TILs in breast tumors predicts favorable prognostic outcomes, particularly in the HER2-overexpression and TN subtypes.
Analysis of long non-coding RNA expression profiles in high-glucose treated vascular endothelial cells
Authors: Xu Erqin , Hu Xiaolei , Li Xiaoli , Jin Guoxi , Zhuang Langen , Wang Qiong , Pei Xiaoyan 
Published: July 2020

Abstract
Background: Diabetes mellitus is often associated with microvascular and macrovascular lesions, and hyperglycemia-induced vascular endothelial cell damage is a key factor. Methods： We investigated long non-coding RNAs (lncRNAs) and mRNAs that are affected by hyperglycemia-induced damage using human umbilical vein endothelial cells (HUVECs) as a model. HUVECs were cultured under high (25 mmol/L)or normal normal (5 mmol/L) glucose conditions for 6 d, and then lncRNAs and protein-coding transcripts were profiled by RNA-seq. Results： Among 40,379 lncRNAs screened, 214 were upregulated (log2 [fold-change] > 1, FDR < 0.05) and 197 were downregulated (log2 [fold-change] < −1, FDR < 0.05) in response to high-glucose. Furthermore, among 28,431 protein-coding genes screened, 778 were upregulated and 998 were downregulated. A total of 945 lncRNA/mRNA pairs were identified, including 126 differentially expressed lncRNAs predicted to target 201 mRNAs, among which 26 were cis-regulatory interactions. The corresponding lncRNA-mRNA network was composed of 354 lncRNA nodes, 1,167 mRNA nodes and 9,735 edges. Dozens of lncRNAs with high degree may play important roles in high-glucose-induced HUVEC damage, including ENST00000600527, NONHSAT037576.2, NONHSAT135706.2, ENST00000602127, NONHSAT200243.1, NONHSAT217282.1, NONHSAT176260.1, NONHSAT199075.1, NONHSAT067063.2, NONHSAT058417.2. Conclusion： These observations may provide novel insights into the regulatory molecules and pathways of hyperglycemia-related endothelial dysfunction in diabetes-associated vascular disease.
Mortality at the Pediatric Emergency Unit of the Mohammed VI Teaching Hospital of Marrakech
Authors: Lahmini Widad , bourrous mounir 
Published: July 2020

Abstract
Background : The death of a child at the emergency ward is one of the most difficult problems that the clinicians of these wards have to deal with. In our country the published data concerning the causes and the factors related to pediatric mortality especially in the pediatric emergency wards is very rare. This study aimed to study the epidemiology of the pediatric mortality in the pediatric emergency department (PED), to determine its rate and identify its most frequent causes. Methods : It is a retrospective and descriptive study, over five years (1st January 2012 and 31st December 2016) including all children aged from 0 to 15 years old who died at the PED in the Mohamed VI Hospital in Marrakech. Results : During the period of the study a total of 172.691 patients presented to the PED, among which 628 died (pediatric mortality rate : 3.63%). The masculine gender was predominant (n=383) with a gender ratio of 1.59. Two-thirds of the patients died in the first 24 hours (n=421). The median of time from admission to death was around 12 hours. Majority of the deceased children (n=471, 75%) were from a low socioeconomic status. The most frequent cause of admissions for deceased patients in the PED was respiratory distress (n=296, 47%) followed by neurological disorders (n=70, 11%). Neonatal mortality (≤ 1 month of age) was predominant (n=472, 75.1%), followed by postnatal mortality (1 month to 1 year old) (n=73, 11.6%). The most frequent causes of pediatric mortality, whatever the age range, were dominated by neonatal pathologies (n=391, 62.3%), followed by infecious causes bronchopulmonary infections included (n=49, 7.7%), birth deformities (n=46, 7.3%) while traumas were merely at 0.9% (n=6). The most frequent causes of neonatal mortality were neonatal infections (n=152, 32.2%) and prematurity (n=115, 24.4%). Conclusion : Our data once again underline the crucial importance of prevention. This requires correct follow-up of the pregnancies, an adequate assistance of births, and perfecting healthcare provision to newborns in order to attain proper assistance.
How to promote exclusive breastfeeding in Ireland: a qualitative study on views of Chinese immigrant mothers
Authors: Chen Haoyue , Zhou Qianling , Cassidy Tanya M. , Younger Katherine M. , Shen Siao , Kearney John M. 
Published: July 2020

Abstract
Background

The exclusive breastfeeding rate in Ireland is very low with extremely slow annual growth. The population of immigrants in Ireland is increasing. Improving exclusive breastfeeding practice among immigrants may contribute to the overall improvement of exclusive breastfeeding rates in Ireland. This study was conducted to elicit recommendations on improving exclusive breastfeeding rate for six months among Chinese immigrants in Ireland.

Methods

Fourteen semi-structured in-depth individual interviews were conducted with Chinese immigrant mothers resident in Ireland, who breastfed exclusively for four to six months.

Results

Themes generated from thematic content analyses were 1) recommendation for mothers: improve self-intention to breastfeed exclusively and self-efficacy, be financially secure before pregnancy, and obtain breastfeeding information proactively. 2) Recommendation for family members, employers and colleagues, and health care professionals: family members should offer sufficient support. Employers should provide hygienic places for breastmilk storage, and set up breastfeeding rooms and nurseries. Irish hospitals need to advocate exclusive breastfeeding, recruit and train multilingual health care professionals, and combine breastfeeding knowledge with practice in the prenatal education. 3) Recommendation for the government: set up breastfeeding rooms and dedicated rooms for breastfeeding groups, and increase the publicity and visibility of breastfeeding in multiple languages. An independent welfare policy for immigrants was also recommended.

Conclusions

The key findings emerging from this study may be considered in the development of breastfeeding promotion strategies in Ireland. Our findings could also have implications for other English-speaking countries with low rates of exclusive breastfeeding.
A family of partial-linear single-index models for analyzing complex environmental exposures with continuous, categorical, time-to-event, and longitudinal health outcomes
Authors: Wang Yuyan , Wu Yinxiang , Jacobson Melanie H. , Lee Myeonggyun , Jin Peng , Trasande Leonardo , Liu Mengling 
Published: July 2020

Abstract
Background: Statistical methods to study the joint effects of environmental factors are of great importance to understand the impact of correlated exposures that may act synergistically or antagonistically on health outcomes. This study proposes a family of statistical models under a unified partial-linear single-index (PLSI) modeling framework, to assess the joint effects of environmental factors for continuous, categorical, time-to-event, and longitudinal outcomes. All PLSI models consist of a linear combination of exposures into a single index for practical interpretability of relative direction and importance, and a nonparametric link function for modeling flexibility. Methods: We presented PLSI linear regression and PLSI quantile regression for continuous outcome, PLSI generalized linear regression for categorical outcome, PLSI proportional hazards model for time-to-event outcome, and PLSI mixed-effects model for longitudinal outcome. These models were demonstrated using a dataset of 800 subjects from NHANES 2003-2004 survey including 8 environmental factors. Serum triglyceride concentration was analyzed as a continuous outcome and then dichotomized as a binary outcome. Simulations were conducted to demonstrate the PLSI proportional hazards model and PLSI mixed-effects model. The performance of PLSI models was compared with their counterpart parametric models. Results: PLSI linear, quantile, and logistic regressions showed similar results that the 8 environmental factors had both positive and negative associations with triglycerides, with a-Tocopherol having the most positive and trans-b-carotene the most negative association. For the time-to-event and longitudinal settings, simulations showed that PLSI models could correctly identify directions and relative importance for the 8 environmental factors. Compared with parametric models, PLSI models got similar results when the link function was close to linear, but clearly outperformed in simulations with nonlinear effects. Conclusions: We presented a unified family of PLSI models to assess the joint effects of exposures on four commonly-used types of outcomes in environmental research, and demonstrated their modeling flexibility and effectiveness, especially for studying environmental factors with mixed directional effects and/or nonlinear effects. Our study has expanded the analytical toolbox for investigating the complex effects of environmental factors. A practical contribution also included a coherent algorithm for all proposed PLSI models with R codes available.
Short Term Changes in Anterior Segment and Retina After Small Incision Lenticule Extraction
Authors: Chen Yanwei , Liao Huaping , Sun Yue , Shen Xi 
Published: July 2020

Abstract
Purpose: To analyze changes of the anterior segment and retina after small incision lenticule extraction (SMILE) in the short term.Methods: Myopia patients scheduled for SMILE were recruited in Ruijin Hospital, Shanghai, China. Basic information of patients such as age, gender, and refractive errors was recorded. Ocular measurements were taken before surgery, 1 day and 1 week after surgery, including axial length (AL), central corneal thickness (CCT), anterior chamber depth (ACD), lens thickness (LT), white to white (WTW), pupil diameter (PD), macular thickness (MT), ganglion cell layer thickness (GCL), retinal nerve fiber layer thickness (RNFL), choroidal thickness (CT), macular vessel density, optic disc vessel density. Results of postoperative were compared to baseline, respectively, with paired student t test, and the results were considered statistically significant when p<0.05. Correlations between sphere and changes of ACD (𝜟ACD) and LT (𝜟LT) were analyzed with the Pearson correlation coefficient.Results: 61 eyes of 31 patients were selected for this study. AL, CCT, ACD, and PD postoperative were significantly reduced (p<0.05), while LT was thickened after surgery (p<0.05). MT at the fovea decreased on 1 day and 1 week after surgery (p<0.05). GCL was no statistical changes after surgery. RNFL was unchanged 1 day after surgery, but the inferior sector was thickened 1 week after surgery. CT was observed thicker at fovea 1 day after surgery and 1.0mm from the fovea in nasal 1 week after surgery. Macular vessel density was significantly decreased on 1 day after surgery and most recovered in 1 week. Optic disc vessel density decreased at the peripapillary part on 1 day after surgery and recovered in 1 week. 𝜟ACD and 𝜟LT were found no significant correlation on 1 day after surgery. 𝜟ACD was negatively correlated with 𝜟LT and sphere on 1 week after surgery (r=-0.847, p<0.000; r=-0.398, p=0.002). 𝜟LT was positively correlated with the sphere on 1 week after surgery (r=0.256, p=0.048).Conclusion: Anterior segment was the most influenced while the retina was also impacted in MT, RNFL, CT, macular vessel density, and peripapillary vessel density.
A Nomogram based on a Patient-reported Outcomes Measure: Predicting the Risk of Readmission for Patients with Chronic Heart Failure
Authors: Han Qiang , Ren Jia , Tian Jing , Yang Hong , Zhang Qing , Wang Ruoya , Zhao Jinghua , Han Linai , Li Chenhao , Yan Jingjing , Wang Ke , Zheng Chu , Han Qinghua , Zhang Yanbo 
Published: July 2020

Abstract
Background

Health-related quality of life, evaluated by a patient-reported outcomes measure, is an important prognostic marker in patients with chronic heart failure. This study aimed to use PROM to establish an effective readmission nomogram for chronic heart failure.

Methods

Using patient-reported outcomes measure as a measurement tool, we conducted a readmission nomogram for chronic heart failure on a prospective observational study comprising 454 patients with chronic heart failure hospitalized between May 2017 to January 2020. Concordance index and calibration curve were used to evaluate the discriminative ability and predictive accuracy of the nomogram. A bootstrap resampling method was used for internal validation of results.

Results

The median follow-up period in the study was 372 days. After a final COX regression analysis, the gender, income, health care, appetite-sleep, anxiety, depression, paranoia, support, and independence were identified and included in the nomogram. The nomogram showed moderate discrimination, with concordance index of 0.737 (95% CI 0.673-0.800). The calibration curves for the probability of readmission for patients with chronic heart failure showed high consistency between the probability, as predicted, and the actual probability.

Conclusions

This model offers a platform to assess the risk of readmission for different populations with CHF and can assist clinicians with personalized treatment recommendations.
Analysis of Operation Procedure and Effect for Emergency Surgery in General Hospital During Novel Coronavirus Pneumonia Period
Authors: Liu Yuchen , Wang Minggang , Shen Yingmo , Chen Jie 
Published: July 2020

Abstract
Background Novel coronavirus pneumonia(NCP) outbreak in Wuhan, China in early 2020, resulted in over 80 thousand infections in China. At present, NCP has an explosive growth in the world. Surgeons could refuse selective operation during the outbreak, but they must face the emergency operation. We hope to avoid the spread of NCP while ensuring efficient treatment of emergency cases. Methods The data of patients with incarcerated hernia admitted to Beijing Chaoyang Hospital during NCP epidemic were analyzed and compared with those in 2019. All cases were divided into NCP group and 2019 group. The operation data and inpatient protection process of emergency cases were analyzed. Result During the NCP epidemic, 17 cases with incarcerated hernia were treated in our department. A Total of 263 cases of the same disease were admitted in 2019. There was no significant difference in age, gender, BMI and hernia type between two groups. No significant difference was observed between the two groups in operation method and hospital stay. The waiting time for emergency operation of NCP group was significantly longer than that of 2019 group (P=0.002). A buffer ward was set up by administrator of hospital during NCP outbreak. Hospitals were divided into "Red area, Yellow area and Green area" artificially, and strict screening consultation system was implemented. There was no case of SARS-nCoV-2 infection in medical staff. ﻿Conclusion It was safe and effective to carry out emergency operation on the premise of screening, protection and isolation during the NCP epidemic. The increased waiting time for operation due to NCP screening did not threaten medical safety of emergency incarcerated hernia patients.
Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC): Does increased intraperitoneal pressure change distribution patterns and penetration depth of doxorubicin in a sheep model?
Authors: Mimouni Myriam , Richard Christophe , Adenot Pierre , Letheule Martine , Tarrade Anne , Sandra Olivier , Dahirel Michèle , Lilin Thomas , Lecuelle Benoit , Gélin Valérie , Cohen Julien , Fauconnier Arnaud , Vialard François , Huchon Cyrille , Chavatte-Palmer Pascale 
Published: July 2020

Abstract
Background:Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC) is an innovative treatment against peritoneal carcinomatosis. Doxorubicin is a common intra-venous chemotherapy used for peritoneal carcinomatosis and for PIPAC. This study evaluated the impact of increased PIPAC intraperitoneal pressure on the distribution and cell penetration of doxorubicin in a sheep model.Methods: Doxorubicin was aerosolized using PIPAC into the peritoneal cavity of 6 ewes (pre-alpes breed): N=3 with 12mmHg intraperitoneal pressure (group 1) and N=3 with 20mmHg (group 2). Samples from peritoneum (N=6), ovarian (N=1), omentum (N=1) and caecum (N=1) were collected for each ewe. The number of doxorubicin positive cells was determined using the ratio between doxorubicine fluorescence-positive cell nuclei (DOXO+) over total number of DAPI positive cell nuclei (DAPI+). Penetration depth (μm) was defined as the distance between the luminal surface and the location of the deepest DOXO+ nuclei over the total number of cell nuclei that were stained with DAPI. Penetration depth (μm) was defined as the distance between the luminal surface and the location of the deepest DOXO+ nuclei.Results: DOXO+ nuclei were identified in 87% of samples. All omental samples, directly localized in front of the nebulizer head, had 100% DOXO+ nuclei whereas very few nuclei were DOXO+ for caecum. Distribution patterns were not different between the two groups but penetration depth in ovary and caecum samples was significantly deeper in group 2. Conclusions:This study showed that applying a higher intra-peritoneal pressure during PIPAC treatment leads to a deeper penetration of doxorubicin in ovarian and caecum but does not affect distribution patterns.
Psychometric Properties of the Moral Injury Symptoms Scale among Chinese Health Professionals during the COVID-19 Pandemic
Authors: Wang Zhizhong , Koenig Harold G , Tong Yan , Wen Jing , Sui Mu , Liu Hongyu , Liu Guangtian 
Published: July 2020

Abstract
Aims To assess the psychometric properties of the 10-item Moral Injury Symptoms Scale-Health Professional (MISS-HP) among healthcare professionals in China. Methods: A total of 583 nurses and 2,423 physicians were recruited from across mainland China. An online survey was conducted using the Chinese version of the MISS-HP from March 27 to April 26, 2020 (during the middle of the COVID-19 pandemic). Reliability was assessed by internal consistency reliability and test-retest reliability. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) was performed to determine scale structure. Results: Cronbach’s α of the scale for both samples was acceptable (0.71 for nurses and 0.70 for physicians), as was test-retest reliability ( ICCs for the individual items ranged from 0.41 to 0.74, with 0.77 for the overall scale in physicians). EFA suggested three factors, and the CFA indicated good fit to the data. Convergent validity was demonstrated with the 4-item Expressions of Moral Injury Scale (r=0.45 for physicians, r=0.43 for nurses). Discriminant validity was demonstrated by correlations with burnout and well-being (r=0.34–0.47), and concurrent validity was suggested by correlations with depression and anxiety symptoms (r=0.37–0.45). Known groups validity was indicated by a higher score in those exposed to workplace violence (B=4.16, 95%CI: 3.21-5.10, p<0.001). Conclusion: The MISS-HP demonstrated acceptable reliability and validity in a large sample of physicians and nurses in mainland China, supporting its use as a screening measure for moral injury symptoms among increasingly stressed health professionals in this country during the COVID-19 pandemic.
Nutrient restriction, inducer of yeast meiosis, induces mammalian meiotic initiation
Authors: Zhang Xiaoyu , Gunewardena Sumedha , Wang Ning 
Published: July 2020

Abstract
From yeasts to mammals, the molecular machinery and chromosome structures carrying out meiosis are frequently conserved. However, the signal to initiate meiosis appears divergent: while nutrient restriction induces meiosis in the yeast system, retinoic acid (RA), a chordate morphogen, and its target, Stra8, are necessary but not sufficient to induce meiotic initiation in mammalian germ cells. Here, by using a combination of genetic, transcriptomic, cytologic approaches in mouse primary spermatogonial culture without the support of gonadal somatic cells, we show that nutrient restriction is both necessary and sufficient to robustly induce Spo11-dependent meiotic DNA double strand breaks (DSBs) and Stra8-dependent meiotic gene programs with RA, recapitulating those of early meiosis in vivo. Moreover, distinct network of 11 nutrient restriction-upregulated transcription factor genes was identified, whose expression does not require RA and is associated with early meiosis in vivo. Thus, our study proposes a conserved model, in which nutrient restriction induces meiotic initiation by upregulating key transcriptional factors for meiotic gene programs, and provides an in vitro platform to recapitulate meiotic initiation that will facilitate research and haploid gamete production.