bluuebunny/crossref_metadata_2025_split huggingface dataset:
The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13
Authors: Mulas Floriana , Wang Xu , Song Shanshan , Nishanth Gopala , Yi Wenjing , Brunn Anna , Larsen Pia-Katharina , Isermann Berend , Kalinke Ulrich , Barragan Antonio , Naumann Michael , Deckert Martina , Schlüter Dirk 
Published: March 2020

AbstractDendritic cells (DCs) are indispensable for defense against pathogens but may also contribute to immunopathology. Activation of DCs upon the sensing of pathogens by Toll-like receptors (TLRs) is largely mediated by pattern recognition receptor/nuclear factor-κB (NF-κB) signaling and depends on the appropriate ubiquitination of the respective signaling molecules. However, the ubiquitinating and deubiquitinating enzymes involved and their interactions are only incompletely understood. Here, we reveal that the deubiquitinase OTU domain, ubiquitin aldehyde binding 1 (OTUB1) is upregulated in DCs upon murine Toxoplasmagondii infection and lipopolysaccharide challenge. Stimulation of DCs with the TLR11/12 ligand T. gondii profilin and the TLR4 ligand lipopolysaccharide induced an increase in NF-κB activation in OTUB1-competent cells, resulting in elevated interleukin-6 (IL-6), IL-12, and tumor necrosis factor (TNF) production, which was also observed upon the specific stimulation of TLR2, TLR3, TLR7, and TLR9. Mechanistically, OTUB1 promoted NF-κB activity in DCs by K48-linked deubiquitination and stabilization of the E2-conjugating enzyme UBC13, resulting in increased K63-linked ubiquitination of IRAK1 (IL-1 receptor-associated kinase 1) and TRAF6 (TNF receptor-associated factor 6). Consequently, DC-specific deletion of OTUB1 impaired the production of cytokines, in particular IL-12, by DCs over the first 2 days of T. gondii infection, resulting in the diminished production of protective interferon-γ (IFN-γ) by natural killer cells, impaired control of parasite replication, and, finally, death from chronic T.encephalitis, all of which could be prevented by low-dose IL-12 treatment in the first 3 days of infection. In contrast, impaired OTUB1-deficient DC activation and cytokine production by OTUB1-deficient DCs protected mice from lipopolysaccharide-induced immunopathology. Collectively, these findings identify OTUB1 as a potent novel regulator of DCs during infectious and inflammatory diseases.
In-situ Monitoring on Micro-hardness of Laser Molten Zone on AISI4140 Steel by Spectral Analysis
Authors: Wang Siyu , Wang Yichen , Liu Changsheng , Mazumder Jyoti 
Published: March 2020

AbstractThe real-time monitoring technology plays a significant role in the field of laser aided manufacturing. It not only ensures the product quality, but also saves time and expenditure on the subsequent testing. To develop a method to monitor the properties of laser molten zone, in this paper, the AISI4140 steel samples were melted by laser with different parameters. At the same time, the plasma spectra were detected during real-time laser processing. The evolutions for both emission spectra and hardness of molten zone were researched in this work. To correlate the intensity of spectral line with the hardness of molten zone, the method of dimensionless analysis was used in this experiment. As the results shown, in a dimensionless system, there was a linear correlation between dimensionless micro-hardness of molten zone (H*) and dimensionless laser energy density(ln(δ*)); the dimensionless micro-hardness could be expressed by a piecewise function using dimensionless intensity of Fe I spectral lines(I*), dimensionless velocity(v*) and dimensionless laser energy density as variables; depending on the quantitative relation among all dimensionless, a monitoring system of hardness of molten zone was established; by testing under different parameters of laser processing, the mean error of prediction is lower than 3.1%. It means the emission spectroscopy can be a potential way to monitor the properties of parts prepared by laser processing.
Loss of Estrogen Receptors is Associated with Increased Tumor Aggression in Laryngeal Squamous Cell Carcinoma
Authors: Verma Anjali , Schwartz Nofrat , Cohen David J. , Patel Vaidehi , Nageris Benny , Bachar Gideon , Boyan Barbara. D. , Schwartz Zvi 
Published: March 2020

AbstractLaryngeal squamous cell carcinoma (LSCC) responds to 17β-estradiol via estrogen-receptor (ER, transcribed from ESR1) dependent mechanisms, but is not recognized as a hormonally responsive cancer. 17β-estradiol production by LSCC cell lines UM-SCC-11A and UM-SCC-12 was examined. Wild type (WT) and ESR1-silenced LSCC cultures and xenografts were examined for 17β-estradiol responsiveness in vivo. 14 LSCC and surrounding epithelial samples at various pathological stages were obtained from patients; ERα and ERβ expression were verified using data from the total cancer genome atlas. UM-SCC-11A and UM-SCC-12 both produce 17β-estradiol, but only UM-SCC-12, not UM-SCC-11A, xenograft tumors grow larger in vivo in response to systemic 17β-estradiol treatments. ERα66 and ERα36 expression inversely correlated with clinical cancer stage and tumor burden. LSCC ERα66 expression was higher compared to surrounding epithelia in indolent samples but lower in aggressive LSCC. ERβ expression was highly variable. High ESR1 expression correlated with improved survival in LSCC. Loss of ERα66 expression inversely correlated with prognosis in LSCC. ERα66 may be a histopathological marker of aggression in LSCC.
Peripheral perfusion index percentiles for healthy newborns by gestational age and sex in China
Authors: Hu Xiao-jing , Ding Jin-xiang , Wang Ying , Niu Conway , Zhang Yi , Zhao Qu-ming , Yan Wei-li , Cao Yun , Huang Guo-ying 
Published: March 2020

AbstractPeripheral perfusion index (PPI) percentiles for newborns serve as an important observation tool in clinical practice, but research pertaining to reference ranges are lacking. The aim of this study was to establish PPI percentiles for healthy newborns by gestational age and sex at 24–48 hours of life. We conducted an observational study and examined PPI values at 24–48 hours of life in 3814 asymptomatic newborns born between 35 and 41 weeks gestation who did not need medical treatment from June 1, 2016 to May 31, 2017 at two maternity hospitals in Shanghai. Linear regression analysis was carried out on the associations between PPI values and variables such as gestational age, sex, and birthweight. Pre-ductal PPI values linearly increased with gestational age (β: 0.072; 95% CI: 0.037, 0.107; P = 0.000). Post-ductal PPI values were also mainly related to gestational age (β: 0.051; 95% CI: 0.018, 0.085; P = 0.003). Smoothed reference curves for pre- and post-ductal PPI values by gestational age and sex were derived from LMS Chart Maker. Our study is the first study to establish PPI percentiles curves for healthy newborns by gestational age and sex at 24–48 hours of life. Further research is required for the implementation of PPI curves into clinical practice.
Erosion characteristics of different reclaimed substrates on iron tailings slopes under simulated rainfall
Authors: Lv Chunjuan , Bi Rutian , Guo Xingxing , Chen Dan , Guo Yansong , Xu Zhanjun 
Published: March 2020

AbstractWater-induced erosion of iron tailings is a serious problem affecting ecological restoration, but, little is known about how the occurrence of erosion on tailings slopes and types of reclaimed substrates that are beneficial to reducing slope erosion. This study measured the slope erosion characteristics of six reclaimed substrates including loose tailings (LT), crusty tailings (CT), tailings incorporating mushroom residues (TM), tailings incorporating soil (TS), tailings incorporating soil and mushroom residues (TSM) and soil (S) in experimental soil flumes under three simulated intermittent rainfall events, with intensity of 60, 90 and 120 mm h−1 for the first, second and third event, respectively. Significant differences (p < 0.05) were found in erosion characteristics among the six reclaimed substrates. TM had the lowest sediment yield but the highest runoff volume without obvious rills. LT, CT and TS had the highest sediment yield rates and severe slope erosion morphology. With the increased number of rainfall events, the runoff rates of the six substrates all increased, but only the sediment yield rates of LT, CT and TS increased, the sediment yield rates of other substrates increased first and then decreased. Therefore, adding agricultural organic wastes such as mushroom residues to tailings and reducing soil addition may be an effective way to reduce erosion and promote ecological restoration in soilless tailings areas.
Effect of VCP modulators on gene expression profiles of retinal ganglion cells in an acute injury mouse model
Authors: Hasegawa Tomoko , Ikeda Hanako Ohashi , Gotoh Norimoto , Iida Kei , Iwai Sachiko , Nakano Noriko , Kakizuka Akira , Tsujikawa Akitaka 
Published: March 2020

AbstractIn glaucoma, retinal ganglion cells are damaged, leading to the progressive constriction of the visual field. We have previously shown that the valosin-containing protein (VCP) modulators, Kyoto University Substance (KUS)121 and KUS187, prevent the death of retinal ganglion cells in animal models of glaucoma, including the one generated by N-methyl-D-aspartate (NMDA)-induced neurotoxicity. KUSs appeared to avert endoplasmic reticulum (ER) stress by maintaining ATP levels, resulting in the protection of ganglion cells from cell death. To further elucidate the protective mechanisms of KUSs, we examined gene expression profiles in affected ganglion cells. We first injected KUS-treated mice with NMDA and then isolated the affected retinal ganglion cells using fluorescence-activated cell sorting. Gene expression in the cells was quantified using a next-generation sequencer. Resultantly, we found that KUS121 upregulated several genes involved in energy metabolism. In addition, we observed the upregulation of Zfp667, which has been reported to suppress apoptosis-related genes and prevent cell death. These results further support the suitability of KUS121 as a therapeutic drug in protecting retinal ganglion cells in ophthalmic disorders, such as glaucoma.
20(S)-ginsenoside Rh2 as agent for the treatment of LMN-CRC via regulating epithelial–mesenchymal transition
Authors: Yuan Yihang , Wang Jue , Xu Ming , Zhang Yunpeng , Wang Zhiqiang , Liang Leilei , Sun Peng 
Published: March 2020

Abstract
The lymph node metastasis of colorectal cancer (LMN-CRC) seriously threatens the prognosis of patients. Chemotherapy, as the most common treatment, results in severe bone marrow suppression. 20(S)-ginsenoside Rh2 (SGRh2), a major effective constituent of ginseng, has demonstrated therapeutic effects on a variety of diseases, including some tumours. SGRh2 treatment had no effect on other organs. Therefore, ginsenosides are considered a safe and effective antineoplastic drug. However, the effects of SGRh2 on LMN-CRC remain unknown. The present study investigated the potential effect of SGRh2 on LMN-CRC in vitro and in vivo. SW480 and CoLo205 cell lines were treated with SGRh2. SGRh2 dose-dependently decreased CRC cell proliferation by CCK-8, colony formation and Edu assays. The Transwell and scratch assays revealed that SGRh2 inhibits the migratory and invasive abilities of CRC cells in a dose-dependent manner. Furthermore, the results of Western blotting revealed that SGRh2 decreased the expression of matrix metalloproteinase (MMP)-2 and MMP9. In terms of the underlying mechanisms, SGRh2 regulates CRC metastasis by affecting epithelial–mesenchymal transition (EMT), which significantly up-regulated epithelial biomarkers (E-cadherin) and down-regulated mesenchymal biomarkers (N-cadherin and vimentin) and EMT transcriptional factors (Smad-3, Snail-1, and Twist-1). In vivo, SGRh2 significantly inhibited LMN-CRC without affecting other normal organs. Immunohistochemical results showed that SGRh2 treats LMN-CRC by regulating EMT. These results demonstrate that SGRh2 has therapeutic potential for LMN-CRC.
DHA inhibits Gremlin-1-induced epithelial-to-mesenchymal transition via ERK suppression in human breast cancer cells
Authors: Sung Nam Ji , Kim Na Hui , Bae Na Young , Jo Hyo Sik , Park Sin-Aye 
Published: March 2020

Abstract
Docosahexaenoic acid (DHA) is an omega-3 fatty acid abundant in fish oils. It is known to have an inhibitory effect on various diseases such as inflammation, diabetes, and cancer. Epithelial-to-mesenchymal transition (EMT) is a process that epithelial cells gain migratory property to become mesenchymal cells involved in wound healing, organ fibrosis, and cancer progression. Gremlin-1 (GREM1) is a bone morphogenetic protein antagonist known to play a role in EMT. However, the role of GREM1 in the induction of EMT in human breast cancer cells and the effect of DHA on GREM1-induced EMT remain unclear. Establishment of GREM1 knockdown cell lines was performed using lentiviral shRNAs. Expression of EMT markers was determined by qRT-PCR and Western blotting. Effect of GREM1 and/or DHA on cell migration was investigated using wound healing assay. The level of GREM1 expression in human breast cancer tissues was determined by Oncomine database mining. GREM1 induced the expression of genes including N-cadherin, vimentin, and Slug. GREM1 promoted the migration of human breast cancer cells. GREM1 enhanced the expression of phosphorylated extracellular signal-regulated kinase (p-ERK) and the ERK activation was involved in EMT. Interestingly, DHA reduced the expression of GREM1. DHA also inhibited the expression of mesenchymal cell-associated genes and cell migration induced by GREM1. Furthermore, DHA suppressed the expression of p-ERK induced by GREM1. These results indicate that GREM1–ERK axis plays a role in EMT in human breast cancer cells and DHA is a putative compound that can inhibit EMT by inhibiting GREM1 signal transduction.
ODUSSEAS: a machine learning tool to derive effective temperature and metallicity for M dwarf stars
Authors: Antoniadis-Karnavas A. , Sousa S. G. , Delgado-Mena E. , Santos N. C. , Teixeira G. D. C. , Neves V. 
Published: March 2020

Aims. The derivation of spectroscopic parameters for M dwarf stars is very important in the fields of stellar and exoplanet characterization. The goal of this work is the creation of an automatic computational tool able to quickly and reliably derive the Teff and [Fe/H] of M dwarfs using optical spectra obtained by different spectrographs with different resolutions.
Methods. ODUSSEAS (Observing Dwarfs Using Stellar Spectroscopic Energy-Absorption Shapes) is based on the measurement of the pseudo equivalent widths for more than 4000 stellar absorption lines and on the use of the machine learning Python package “scikit-learn” for predicting the stellar parameters.
Results. We show that our tool is able to derive parameters accurately and with high precision, having precision errors of ~30 K for Teff and ~0.04 dex for [Fe/H]. The results are consistent for spectra with resolutions of between 48 000 and 115 000 and a signal-to-noise ratio above 20.
Trans-iron Ge, As, Se, and heavier elements in the dwarf metal-poor stars HD 19445, HD 84937, HD 94028, HD 140283, and HD 160617
Authors: Peterson R. C. , Barbuy B. , Spite M. 
Published: March 2020

Context. The spectra of unevolved metal-poor halo stars uniquely reflect the elemental abundances incorporated during the earliest Galactic epoch. Their heavy-element content is well understood as the products of neutron capture on iron-peak elements. However, for the lightest trans-iron elements with atomic number 30 <  Z <  52, they show striking abundance patterns that defy model predictions. Understanding their sources may illuminate the diverse halo, thick disk, or extragalactic origins of metal-poor stars.
Aims. The primary goal is the derivation of halo dwarf abundances and their uncertainties for six trans-iron elements from UV spectra, plus optical abundances for four additional trans-Fe elements and two well-understood heavier elements.
Methods. For five metal-poor dwarfs, we analyzed high-resolution UV spectra from the Hubble Space Telescope Imaging Spectrograph, supplemented by archival optical echelle spectra. Two independent analyses adopted different programs, models, and line lists, clarifying systematic errors.
Results. The results from the separate UV analyses are in good agreement. The largest source of discrepancy is the placement of the UV continuum. Once rectified, the separate results agree to 0.2 dex for moderately unblended, moderately strong lines. Similar agreement is found with previous works, except where new data and line selection become important, notably our exclusion of trans-Fe lines blended by newly identifed Fe I lines.
Conclusions. Improved line lists lead to low As/Ge ratios that no longer require an early arsenic enhancement. All five stars exhibit a high Mo/Ge abundance ratio, independent of Mo/Fe. The trans-Fe elements show an odd-even effect: an odd-Z element abundance is depressed relative to those of adjacent even-Z elements. Its suggested metallicity dependence is supported by previous studies of Sr-Y-Zr. Some theoretical yields show a metallicity-dependent odd-even effect, but none have predicted a constant Mo/Ge abundance ratio. Our work thus highlights the complexity of predicting the production of light trans-Fe elements in metal-poor stars.
Imaging of an Indeterminate Pancreatic Mass
Authors: Eapen Anu , Chandramohan Anuradha , John Reetu , Simon Betty , Putta Tharani , Joseph A. J. 
Published: March 2020

AbstractSeveral conditions ranging from neoplasm to inflammatory disorders can present as a pancreatic mass on imaging. While classic imaging features help us make an accurate diagnosis, we are often confronted by indeterminate pancreatic masses that pose diagnostic dilemma. In this review, we describe the typical imaging features of common mass forming conditions of pancreas followed by description of atypical imaging appearances, which make a pancreatic mass indeterminate. We also emphasize on imaging features useful in differentiating one from the other.
Application of Personalized Navigation Templates to Oxford Single Condylar Replacement in a Chinese Population
Authors: Gan Yudong , Zhuang Jian , Jiang Wenxiao , Sun Wei , Zhang Yijun 
Published: March 2020

AbstractThe purpose of this study is to develop and validate a novel patient-specific navigational template for unicompartmental knee arthroplasty (UKA). A total of 120 patients who underwent UKA were randomized and divided into the conventional method and navigational template groups. In the navigational template group, patient-specific navigational templates were designed and used intraoperatively to assist 60 patients with UKA. Information on operation time and blood loss was recorded by an independent operating room nurse. After surgery, the positions of the prostheses were evaluated using X-rays. All navigation templates were found to fit its corresponding biomodel appropriately without any free movement. The navigational template exhibited significantly better accuracy than the conventional method. Statistically significant differences in the femoral prosthesis flip angle, femoral prosthesis flexion angle, and tibial plateau varus and valgus angle were observed between the two groups (p = 0.022, 0.042, 0.043, respectively). In addition, the mean operation time was statistically and significantly lower in the navigational template group than in the conventional group (p = 0.035). This study introduces a novel navigational template to UKA, the accuracy of which is proven by clinical operation. This is a Level III, therapeutic study.
Neonatal Seizures: Diagnosis, Etiologies, and Management
Authors: Ziobro Julie , Shellhaas Renée A. 
Published: March 2020

AbstractNeonates are exquisitely susceptible to seizures due to several physiologic factors and combination of risks that are uniquely associated with gestation, delivery, and the immediate postnatal period. Neonatal seizures can be challenging to identify; therefore, it is imperative that clinicians have a high degree of suspicion for seizures based on the clinical history or the presence of encephalopathy with or without paroxysmal abnormal movements. Acute symptomatic neonatal seizures are due to an acute brain injury, whereas neonatal-onset epilepsy may be related to underlying structural, metabolic, or genetic disorders. Though initial, acute treatment is similar, long-term treatment and prognosis varies greatly based on underlying seizure etiology. Early identification and treatment are likely important for long-term outcomes in acute symptomatic seizures, though additional studies are needed to understand optimal seizure control metrics and the ideal duration of treatment. Advances in genetic medicine are increasingly expanding our understanding of neonatal-onset epilepsies and will continue to open doors for personalized medicine to optimize outcomes in this fragile population.
Liver Transplantation for Locally Advanced Hepatocellular Carcinoma
Authors: Kawamura Norio , Taketomi Akinobu 
Published: March 2020

AbstractSince the Milan criteria were accepted as the gold standard, liver transplantation has been widely performed as a curative treatment for early-stage hepatocellular carcinoma (HCC). The outcome of liver transplantation in early-stage HCC is excellent; however, the Milan criteria are strict, and therefore, only limited numbers of patients can benefit from liver transplantation. Many HCC patients are diagnosed at an advanced stage, which falls outside the Milan criteria, so it has been proposed over the last two decades that liver transplant surgeons should perform liver transplantation in locally advanced HCC, when presenting without recurrence. Several trials exploring the upper limits of liver transplantation have been performed, and extensive research on tumor biology has enabled the expansion of liver transplant indication for HCC. Simultaneously, locoregional therapy for advanced HCC was found to be an effective procedure when used to distinguish potentially transplantable patients. This treatment approach, known as a downstaging strategy, has been developed over the last two decades and became an essential treatment option for locally advanced HCC. In this article, the current strategies of liver transplantation for the treatment of locally advanced HCC are reviewed.