bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postoperative Effects of Continuous Dexmedetomidine Infusion During Gynecological Laparoscopy: A Randomized, Placebo-Controlled, Double-Blind, Single Center Clinical Trial
Authors: Liu SiYuan , Wang Binbin , Gao Yongtao , Xu Xingguo 
Published: April 2020

Abstract
Background: Dexmedetomidine(DEX) has proven to be an effective adjuvant to anesthetics owing to its sympatholytic, analgesic, and sedative properties. This study was designed to investigate the postoperative effects of continuous DEX infusion during gynecological laparoscopy and to evaluate the safety of this treatment. Methods: Sixty patients undergoing selective gynecological laparoscopy with general anesthesia were randomly assigned into DEX group (group D )and normal saline group (group N). DEX was infused at 0.5ug/kg/h during anesthesia maintenance in group D while normal saline was infused at the same rate in group N. The primary outcome measures were heart rate and blood pressure at extubation, 1, 2, 3, 4, 5, 6, 7, 8, 12, 24 and 48 hours after operation, NRS scores at 1, 2, 3 ,4, ,8,12,24 hours after surgery and OAA/S scores after extubation. The secondary outcome measures were time to extubation, propofol and remifentanil dosage during anesthesia, postoperative adverse events, and postoperative hospital stay.Results: Systolic and diastolic blood pressures were lower in group D at extubation when compared with group N (P<0.05), but not different at the first postoperative hour or after. HR was lower both at extubation and the first postoperative hour in group D (P<0.05). NRS scores were lower in patients given DEX both at rest and movement at the first postoperative hour (P<0.05). Time to extubation and OAA/S scores after extubation were not different between groups. Postoperative respiratory depression, shivering and pruritus didn’t occur in this research. There were no significant differences in the incidence rate of postoperative adverse events or postoperative hospital stay between the groups.Conclusions: Continuous infusion of DEX during gynecological laparoscopy provided better hemodynamic stability at extubation and the effects on HR continued until 1 hour after operation. DEX administration was associated with lower NRS scores at the first postoperative hour without excessive sedation. DEX is a useful and safe adjuvant for general anesthesia during gynecological laparoscopy.
Clinicopathological significance of Sox10 expression in triple-negative breast carcinoma
Authors: Jin Linfang , Qin Chenglin , Qi Xiaowei , Hong Tingting , Yang Xiaodong , Zhu Xun 
Published: April 2020

Abstract
Purpose The present study aimed to investigate the Sox10 expression in the pathological diagnosis of triple-negative breast cancer (TNBC). Furthermore, its correlation with the clinicopathological characteristics and disease-free survival rate in patients with TNBC was also evaluated to identify the diagnostic utility of Sox10 as a reliable biomarker for diagnosis and prognosis of TNBC. Methods Using immunohistochemistry, we identified the expression of Sox10, GATA-3, FOXA1, GCDFP15 and MGB in 376 cases of primary invasive breast cancer, and 77 cases of metastatic breast cancer. The expression of Sox10 in different molecular subtypes of primary invasive breast cancer and metastatic breast cancer were also compared. Furthermore, the correlation between Sox10 expression and clinicopathological parameters and disease-free survival (DFS) of patients with primary TNBC were also analyzed. Results Expression of Sox10 was only detected in the myoepithelial cells of normal breast, but not in any other types of cells, including luminal cell and fibroblasts. The positive rate of Sox10 in primary and metastatic TNBC was significantly higher than that in the other two types (P < 0.001, P < 0.001, respectively). The sensitivity and specificity of Sox10 expression in primary TNBC and metastatic TNBC were significantly lower than GATA-3, significantly higher than FOXA1, GCDFP15, and MGB (P < 0.001, P = 0.0004, P = 0.0064, P = 0.0229, respectively). In 71 cases of primary TNBC, a higher expression rate of Sox10 was significantly associated with high-grade tumors, late-stage tumors, and tumors with involvement of four or more lymph node metastases (P = 0.0145, P = 0.0105, P = 0.0249, respectively). Conclusion Sox10 may be used as a novel reliable putative marker for the diagnosis of TNBC. Notably, Sox10 combined with GATA-3 expression may serve as a supplementary differential diagnostic biomarker for primary and metastatic TNBC. Besides, Sox10 may be a good predictor of the prognosis of primary and metastatic TNBC. This study also highlights the significance of targeting Sox10 as a promising potential therapeutic target gene for TNBC therapy.
Integrative characterization and validation of tumor-associated immune signature combined with immune-infiltration analysis in clear cell renal cell carcinoma
Authors: Chen Hongxi , Xie Jinliang , Jin Peng 
Published: April 2020

Abstract
Background We aimed to explore and validate a prognostic immune signature for predicting the prognosis of ccRCC patients, combined with immune-infiltration analysis. Methods We obtained the multi-omics data from public datasets. Differential analysis was performed by edgeR package. Prognostic immune signature was identified by univariate Cox analysis, and we constructed an integrative tumor-associated immune Genes (TAIG) model from the multivariate Cox results. Functional analysis was conducted to uncover the related crosstalk. Importantly, we implemented the CIBERSORT algorithm to estimate the immune cell fractions in ccRCC samples and analyzed the differential abundance of tumor-infiltrating immune cells in two TAIG groups using Wilcoxon rank-sum test. The prognostic role of differential immune cells was further assessed by Kaplan-Meier analysis. In addition, we investigated the associations of single immune signature with specific immune cells. Results A total of 628 ccRCC patients were included in our integrative analysis, including 537 ccRCC patients in discovery group and 91 patients in validation group. Then, we identified the 14 key immune signatures. The AUC was 0.802 and patients with higher TAIG suffered from worse prognosis. Correlation analysis indicated that TAIG correlated tightly with clinical variables and TMB. Moreover, functional analysis also implicated the immune-related GO items or crosstalk. Therefore, we discovered the relationships of TAIG with tumor-infiltrating immune cells. The differential abundance of immune cells showed significant prognostic difference consisted of memory activated CD4 + T cell, T follicular helper cells, T regulatory cell, and so on. Moreover, we also characterized the associations between identified signature with specific immune cells. Finally, the 5-year AUC in ICGC cohort was 0.72, suggesting the robustness of TAIG that we constructed. Conclusions Totally, our team characterized the tumor-associated immune signature in ccRCC and further uncovered the prognostic tumor-infiltrating immune cells related with TAIG, providing a comprehensive foundation for investigating mechanisms or individualized immunotherapy.
Sciellin stimulates gallbladder cancer proliferation and tumor-association thrombosis via formation of neutrophil extracellular traps
Authors: Li Yang , Ren Tai , Yang Bo , Miao Huijie , Liu Liguo , Li Yongsheng , Yuan Ruiyan , Song Xiaoling , Cai Chen , Yang Yang , Hu Yunping , Chen Chen , Jiang Chengkai , Zhang Yijian , Liu Ying-bin 
Published: April 2020

Abstract
Background：Apart from primary tumor development and metastasis, cancer-associated thrombosis is the second cause of cancer death in solid tumor malignancy. However, the mechanistic insight into the development of gallblader cancer (GBC) and cancer-associated thrombosis remains unclear. This study aimed to investigate the mechanistic role of Sciellin （SCEL） in GBC cell proliferation and the development of venous thromboembolism. Methods: The expression level of SCEL was determined by immunohistochemical staining. Roles of SCEL in gallbladder cancer cell were determined by molecular and cell biology methodsResults: SCEL was markedly upregulated in GBC and associated with advanced TNM stages and a poor prognosis. Furthermore, SCEL interacted with EGFR and stabilized EGFR expression that activates downstream PI3K and Akt pathway, leading to cell proliferation. In addition, SCEL induces tumor cell IL-8 production that stimulates the formation of neutrophil extracellular traps (NETs), accelerating thromboembolism. In xenografts, SCEL-expressing GBCs developed larger tumors and thrombosis compared with control cells. Conclusions: The present results indicate that SCEL promotes GBC cell proliferation and induces NET-associated thrombosis , thus serving as a potential therapeutic target.
MiRNA-485-5p inhibited the proliferation and promoted the apoptosis of hepatocellular carcinoma cells by targeting MUC1
Authors: Li Ming , Zhu Dong , Peng Xiaozhen , Liu Pinyue , Yang Fen , Luo Hai , Zhang Xuemei , Liu Fang , Wei Le 
Published: April 2020

Abstract
Background The downregulation of miRNA-485-5-p was related to prognosis of various cancers, including hepatocellular carcinoma (HCC), while MUC1 was aberrantly expressed in many cancers and could be a biomarker for prognosis and diagnosis of cancers. The aim of the study was to investigate whether the miRNA-485-5p inhibited proliferation and promoted apoptosis by targeting MUC1 in HCC cells.Methods The expression of miRNA-485-5p in patients with HCC was measured by qPCR, and the epxression of MUC1 was detected by qPCR, western blot and immunohistochemistry. Bioinformatics and dual luciferase assay verified the targeting relationship between miRNA-485-5p and MUC1 in HCC cells. Cell proliferation was detected by CCK-8 assay. Cell apoptosis was detected by flow cytometry.Results The expression of miRNA-485-5p in HCC tissues is downregulated than that in adjacent tissue. However, the exprssion of MUC1 is opposite to the expression of miRNA-485-5p in the patients with HCC. Silencing MUC1 and overexpression miRNA-485-5p both inhibit proliferation and promote apoptosis in HCC cells. MiRNA-485-5p directly targete to the binding site of the MUC1 3’UTR and downregulated the expression of MUC1. Overexpression of MUC1 and miRNA-485-5p reverse the effect of miRNA-485-5p on the cell proliferation and apoptosis.Conclusion MiRNA-485-5p inhibits the proliferation and facilitates the apoptosis by negatively regulating the expression of MUC1 in HCC cells.
Electrical properties and aging behavior of nickel manganite thin films co-doped with Cu and Zn annealed at low temperature
Authors: Le Duc Thang , Ju Heongkyu 
Published: April 2020

Abstract
Nickel-manganite-based thin films with a negative temperature coefficient of resistance (NTCR) characteristic were prepared from (Ni 0.2 Mn 2.8– x Cu x )Cl 2 (0.010£ x £0.040) solutions using the liquid flow deposition (LFD) method. The influence of Cu on the electrical properties of the films annealed at 400 °C was investigated. Adding Cu ( x = 0.025) was found to quite effectively lower the electrical resistivity ( ρ ) of the films to 200 W cm, whereas their sensitivity behavior was significantly degraded. Interestingly, the absolute temperature coefficient of resistance ( TCR ) of the films was enhanced by further adding 0.01 mol Zn along with Cu, without a remarkable increase in ρ . TCR = 2.98% K – 1 and ρ = 880 W cm at room temperature were attained for the Zn-containing specimen with 0.020 mol Cu doping. Moreover, the films with (Zn,Cu) co-doped compositions showed a much higher electrical stability than the Zn-free films.
Computerized tomography scan findings of a patient with severe enterocolitis associated with the coronavirus disease 2019: a case report
Authors: Takifuji Katsunari , Terasawa Hiroshi , Oka Masami , Sahara Masaki , Hara Tomoko , Itoh Hidekazu 
Published: April 2020

Abstract
Background: Currently, there is no available study about the occurrence of enterocolitis associated with the coronavirus disease 2019 (COVID-19) among adults. Case presentation: A 56-year-old man had lower abdominal pain, distension, diarrhea, and bloody stool. Moreover, the patient presented with fever and general fatigue. Laboratory examination did not reveal leukocytosis nor neutrophilia. However, the patient’s C-reactive protein level increased to 4.9 (normal range: 0.0–0.3) mg/dL. Both sputum and stool cultures had normal results. However, abdominal computed tomography (CT) scan revealed wall thickness mainly in the submucosal layer of the left part of the large intestine. Thus, real-time polymerase chain reaction test of throat swabs was performed for the diagnosis of COVID-19, and positive results were obtained. Conclusions: We report the first case of enterocolitis correlated to COVID-19, and the patient had positive abdominal CT scan findings. This imaging modality could be effective for the diagnosis of enterocolitis associated with COVID-19.
Migração feminina brasileira e a experiência do envelhecimento em Portugal: sexismo e outros “ismos”
Authors: Craveiro Queiroz Camila , Cabecinhas Rosa , Cerqueira Carla 
Published: April 2020

Este artigo busca compreender como migrantes brasileiras, maiores de 50 anos, residentes em Portugal, percebem os estereótipos que lhes são imputados a partir do olhar do endogrupo, neste caso, a sociedade de acolhida. Por meio de entrevistas de Histórias de Vida, as quais analisamos sob o viés interseccional e o paradigma descolonial, contextualizamos os temas de maior recorrência nas falas destas migrantes, a saber: os meta-estereótipos, o mercado laboral, o envelhecer e algumas das estratégias de agência utilizadas nas tentativas de integração.
Palavras-chave: migrantes; gênero; racismo; idadismo; interseccionalidade.
Denaturant Induced Equilibrium Unfolding and Conformational Transitional Studies of Germinated Fenugreek β-Amylase Revealed Molten Globule like State at Low pH
Authors: Agrawal Dinesh Chand , Yadav Anjali , Khan Mohd. Asim , Kundu Suman , Kayastha Arvind M. 
Published: April 2020

Background:
β-Amylase (EC 3.2.1.2) is a maltogenic enzyme, which releases β-maltose
from the non-reducing end of the substrates. The enzyme plays important roles for the production
of vaccine, maltiol and maltose rich syrups. Apart from these applications the enzyme protects
cells from abiotic as well as oxidative damage. The enzyme is βwell characterized in βplants and
microbes and crystal structures of β-amylases βhave been βobtained from sweet potato, soybean
and Bacillus cereus.

Objective:
Find out correlation between structural and functional stability induced by change in
pH, temperature and chaotropes.

Methods:
Activity, intrinsic fluorescence, extrinsic fluorescence, near- and far- ultraviolet circular
dichroism spectroscopic measurements were performed.

Results:
Peaks about 208 nm and 222 nm obtained by near-ultraviolet circular dichroism correspond
to α-helix whereas peak at 215 nm shows presence of β-sheet. At pH 2.0, absence of tertiary
structures, exposed of hydrophobic regions and presence of substantial secondary structures, revealed
the existence of molten globule like state. Temperature induced denaturation studies
showed that the enzyme was stable up to 75ºC and the process was found to be irreversible in nature.
Chaotropes dependent equilibrium unfolding studies revealed that at low concentration of
chaotropes, ellipticity and intrinsic fluorescence βintensity were βdecreased βwhereas βenzymatic
activity remained unchanged, which revealed fenugreek β-amylase is multi-domains enzyme and
catalytic βdomain βis more βstable compare to non-catalytic domain. Moreover, the transition was
sigmoidal and non-coincidental.

Conclusion:
Results indicate the probable existence of intermediate states that might perform significant
role in physiological process and biotechnological applications.
Computational Overview of Mycobacterial Thymidine Monophosphate Kinase
Authors: Gul Sana , Khalil Ruqaiya , Zaheer Ul-Haq  , Mubarak Mohammad S. 
Published: April 2020

:
Tuberculosis (TB) ranks among the diseases with the highest morbidity rate with significantly high
prevalence in developing countries. Globally, tuberculosis poses the most substantial burden of mortality. Further,
a partially treated tuberculosis patient is worse than untreated; they may lead to standing out as a critical obstacle
to global tuberculosis control. The emergence of multi-drug resistant (MDR) and extremely drug-resistant (XDR)
strains, and co-infection of HIV further worsen the situation. The present review article discusses validated targets
of the bacterial enzyme thymidine monophosphate kinase (TMPK). TMPKMTB enzyme belongs to the nucleoside
monophosphate kinases (NMPKs) family. It is involved in phosphorylation of TMP to TDP, and TDP is
phosphorylated to TTP. This review highlights structure elucidation of TMP enzymes and their inhibitors study
on TMP scaffold, and it also discusses different techniques; including molecular docking, virtual screening, 3DPharmacophore,
QSAR for finding anti-tubercular agents.
FoodSimplex as a Mean to Improve Portuguese Restaurants’ Goods Manufacturing Practices - Audit and Microbial Assessment
Authors: Baltazar Ana L. , Ferreira Ana , Manyes Lara , Mañes Jordi 
Published: April 2020

Background:
The consumption of meals at resturants has increased mass catering establishments.
Such food services may present several risk factors for foodborne diseases, which have a
substantial negative impact on public health. The smallest business with no technical or financial
support struggles to comply with food safety European Regulation. The aim of this four years study
was to assess Good Manufacture Practices (GMP) in Portuguese restaurants applying a designed
food safety methodology, FoodSimplex, through audit data to determine good manufacture practices,
and microbiological sampling of the meals before and after its implementation, by food groups according
to their risk.

Methods:
FoodSimplex is a combination of four stages methodology, based on Hazards Analysis and
Critical Control Point (HACCP), combined with technical-functional and food safety audits, with
pretested checklist, training and microbial analysis (Mesophylic bacteria, Listeria monocytogenes, E.
coli, Coagulase-positive staphylococci and Salmonella spp) of the meals served.

Results:
Results of the food safety audit for GMP parameters showed a general improvement with
80% compliance with the checklist subitems. The ready-to-eat food samples presented, regarding the
total mesophilic aerobes count, a statistical significant change to acceptable and satisfactory condition
(p-value= 0.029). 100% compliance, with acceptable and satisfactory results was observed for
Listeria monocytogenes (p-value= 0.180) and for E. coli, coagulase-positive Staphylococci and Salmonella
spp., all the food samples presented satisfactory results in the study (100%).

Conclusion:
At the end of the investigation period, a decrease in bacterial populations in food samples
was observed with an improvement of the GMP audit data which indicated that the FoodSimplex
methodology improved the food safety status of these establishments.
Effect of Javanese Turmeric (Curcuma xanthorrhiza) Extract on Hepatitis Model of Alcohol-Induced Mice
Authors: Puteri Agnes Ilene Suprapto , Sandhika Willy , Hasanatuludhhiyah Nurina 
Published: April 2020

Alcoholic hepatitis is a common cause of non-viral hepatitis. Javanese turmeric (Curcuma xanthorrhiza) contains xanthorrhizol and curcuminoid that have antioxidant and anti-inflammatory effects. The purpose of this research was to detect the effect of Curcuma xanthorrhiza in decreasing hydropic degeneration and lymphocytes in alcohol-induced mice. This experimental research used post-test only controlled design. Randomly, 35 mice were divided into five groups. Four groups were induced by alcohol 30% of 4ml/kgBW in 10 days, and one group was normal group (negative control). Three of the four alcohol-induced groups were given Curcuma xanthorrhiza extract at doses of 42, 84, and 168 mg/kgBW in 10 days, 1 hour after alcohol induction. Mice livers were taken for Hematoxylin Eosin (HE) staining.Â Hydropic degeneration and lymphocytes in the liver lobules were examined under a microscope. The statistical analysis used in this research was the Kruskal-Wallis, the Mann-Whitney test, and the Independent Sample T Test. P-value is significant if <0.05. The result of this research were all doses of Javanese turmeric extract significantly reduced hydropic degeneration. Doses of 84 and 168 mg/kgBW also reduced inflammatory lymphocytes (p<0.05). The dose of 168 mg/kgBW reduced the lymphocyte inflammation maximally. There was no significant difference between increasing Javanese turmeric dose and decreasing hydropic degeneration and lymphocytes (p>0.05). This research shows that Javanese turmeric (Curcuma xanthorrhiza) can attenuate hepatitis by decreasing hydropic degeneration and lymphocytes in alcohol-induced liver.
ДИНАМІКА ЕЛЕКТРОМІОГРАФІЧНИХ ПОКАЗНИКІВ MUSCULUS MASSETER ТА MUSCULUS TEMPORALIS ПІД ВПЛИВОМ СТИМУЛЯЦІЇ ГРУПИ М’ЯЗІВ, ЯКІ ЗАБЕЗПЕЧУЮТЬ ТОНКУ КООРДИНАЦІЮ ЖУВАЛЬНОЇ МУСКУЛАТУРИ В ПРОЦЕСІ ОРТОПЕДИЧНОГО ЛІКУВАННЯ ПАЦІЄНТІВ З ПОВНОЮ ВІДСУТНІСТЮ ЗУБІВ
Authors: Сарапук В. І. , Рожко М. М. 
Published: April 2020

Резюме. Мета: вивчити можливість застосування набору стандартних взірців H. Landt для тесту на здатність до тонкої координації жувальної мускулатури – МА-тест (Muscular-Ability) з ціллю підвищення адаптаційних можливостей до повних знімних пластинкових протезів (ПЗПП). Даний процес адаптації ми назвали МА-стимуляція.
Методи: електроміографія (показник сумарної амплітуди роботи musculus masseter та musculus temporalis (мкв)). Проведено дослідження у двох групах пацієнтів із повною відсутністю зубів. 1 група – 30 пацієнтів, яким ортопедичне лікування проводилося без попередньої МА-стимуляції. 2 група – 60 пацієнтів, яким перед ортопедичним лікуванням протягом 14 днів проводилася МА-стимуляція.
Результати. У 2 групі показник у стані відносного фізіологічного спокою знизився на 23,5 %, через 1 місяць після ортопедичного лікування ще на 14,1 %, через 3 місяці – ще на 9,2 %; при максимальному стисненні щелеп відбулося зростання показника на 29,8 %, через 1 і 3 місяці зростання досягнуло відповідно 36,4 % і 44,7 %; при максимальному стисненні щелеп з ватними валиками відбулося зростання показника на 26,8 %, через 1 і 3 місяці зростання досягнуло відповідно 27,1 % і 42,2 %.
У 1 групі показник у стані відносного фізіологічного спокою знизився через 1 місяць після ортопедичного лікування на 18,1 %, а через 3 місяці ще на 20,9 %; при максимальному стисненні щелеп зростання показника через 1 місяць не відбулося, а через 3 місяці зростання відбулося на 25,2 %; при максимальному стисненні щелеп з ватними валиками зростання відбулося тільки через 3 місяці після ортопедичного лікування на 25,1 %.
Висновки. У пацієнтів з повною відсутністю зубів МА-стимуляція сприяє швидкому активному підвищенню адаптаційних процесів до використання ПЗПП.