bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic analysis of Aspergillus niger 3.316 under heat stress
Authors: Deng Xiangyu , Du Bin , Zhu Fengmei , Gao Yanan , Li Jun 
Published: February 2020

Abstractβ‐Glucosidase production by Aspergillus niger is accompanied by an inevitable temperature increase in the industrial fermentation environment. Hence, the synthetic process of β‐glucosidase is negatively affected. However, our understanding of the heat stress response (HSR) mechanism in A. niger is still incomplete. The current study explored the intracellular proteome profile of A. niger 3.316 in group T (50°C stress) and group C (30°C control) using two proteomic approaches (isobaric tags for relative and absolute quantitation [iTRAQ] and label‐free) and examined the expression of four proteins using a parallel reaction monitoring (PRM) approach. Based on the result of the iTRAQ proteomic analysis, 1,025 proteins were differentially expressed in group T compared to group C. Using the label‐free approach, we only focused on 77 proteins with significant changes in their protein expression levels. In addition, we performed bioinformatics analysis on all these proteins and obtained detailed gene ontology (GO) enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway results. Under heat stress conditions, the relative expression levels of proteins with protection and repair functions were upregulated in A. niger 3.316. These proteins were involved in metabolic pathways, oxidative phosphorylation, porphyrin and chlorophyll metabolism, pyruvate metabolism, and the citrate cycle (TCA cycle). The insights obtained from the presented proteomics and bioinformatics analyses can be used to further explore the HSR mechanism of A. niger.
Procyanidin C1 Inhibits Melanoma Cell Growth by Activating 67‐kDa Laminin Receptor Signaling
Authors: Bae Jaehoon , Kumazoe Motofumi , Murata Kyosuke , Fujimura Yoshinori , Tachibana Hirofumi 
Published: February 2020

ScopeProcyanidin C1 (PC1) is an epicatechin trimer found mainly in grapes that is reported to provide several health benefits. However, little is known about the molecular mechanisms underlying these benefits. The aim of this study is to demonstrate the molecular mechanisms by which PC1 operates.Methods and resultsA 67‐kDa laminin receptor (67LR) is identified as a cell surface receptor of PC1, with a Kd value of 2.8 µm. PC1 induces an inhibitory effect on growth, accompanied by dephosphorylation of the C‐kinase potentiated protein phosphatase‐1 inhibitor protein of 17 kDa (CPI17) and myosin regulatory light chain (MRLC) proteins, followed by actin cytoskeleton remodeling in melanoma cells. These actions are mediated by protein kinase A (PKA) and protein phosphatase 2A (PP2A) activation once PC1 is bound to 67LR.ConclusionIt is demonstrated that PC1 elicits melanoma cell growth inhibition by activating the 67LR/PKA/PP2A/CPI17/MRLC pathway.
Advanced Cell Mapping Visualizations for Single Cell Functional Proteomics Enabling Patient Stratification
Authors: Bowman Nick , Liu Dong , Paczkowski Patrick , Chen Jon , Rossi John , Mackay Sean , Bot Adrian , Zhou Jing 
Published: February 2020

AbstractHighly multiplexed single‐cell functional proteomics has emerged as one of the next‐generation toolkits for a deeper understanding of functional heterogeneity in cell. Different from the conventional population‐based bulk and single‐cell RNA‐Seq assays, the microchip‐based proteomics at the single‐cell resolution enables a unique identification of highly polyfunctional cell subsets that co‐secrete many proteins from live single cells and most importantly correlate with patient response to a therapy. The 32‐plex IsoCode chip technology has defined a polyfunctional strength index (PSI) of pre‐infusion anti‐CD19 chimeric antigen receptor (CAR)‐T products, that is significantly associated with patient response to the CAR‐T cell therapy. To complement the clinical relevance of the PSI, a comprehensive visualization toolkit of 3D uniform manifold approximation and projection (UMAP) and t‐distributed stochastic neighbor embedding (t‐SNE) in a proteomic analysis pipeline is developed, providing more advanced analytical algorithms for more intuitive data visualizations. The UMAP and t‐SNE of anti‐CD19 CAR‐T products reveal distinct cytokine profiles between nonresponders and responders and demonstrate a marked upregulation of antitumor‐associated cytokine signatures in CAR‐T cells from responding patients. Using this powerful while user‐friendly analytical tool, the multi‐dimensional single‐cell data can be dissected from complex immune responses and uncover underlying mechanisms, which can promote correlative biomarker discovery, improved bioprocessing, and personalized treatment development.
The Effects of Aniline Stabilizers on Nitrocellulose Based on Isothermal Thermal Decomposition
Authors: Luo Liqiong , Jin Bo , Chai Zuohu , Huang Qiong , Chu Shijin , Peng Rufang 
Published: February 2020

AbstractThe effects of various aniline stabilizers on the thermal stability of nitrocellulose (NC) were investigated via isothermal thermal decomposition dynamics. The pressure‐time curves of NC/stabilizer composites were found to have a distinct inflection point, which was not observed on the pressure‐time curves of NC. The effects of stabilizers on the isothermal thermal decomposition kinetics of NC were evaluated using Arrhenius equation and model‐fitting method, and the effect of stabilizers on the storage time of NC was estimated using Berthelot equation. Results showed that the activation energy of NC/MNA, NC/ENA, NC/i‐PNA, and NC/n‐BNA before the inflection point were 162.9, 153.3, 147.4, and 150.6 kJ mol−1, respectively, which were significantly increased compared with that of pure NC (144.7 kJ mol−1). Furthermore, the time required for the decomposition extent of NC/MNA, NC/ENA, NC/i‐PNA, and NC/n‐BNA to reach 0.1 % were 24.9, 11.2, 16.7, and 11.2 years, respectively, at 298.15 K, which was longer than that required of NC (10.6 years). UV‐vis revealed that the stabilizers were almost consumed when the NC/stabilizer thermal decomposition pressure‐time curve reached the inflection point. Based on the above results, the stability order was MNA>i‐PNA>ENA>n‐BNA as the criterion for time of the inflection point.
Analyses of protein cores reveal fundamental differences between solution and crystal structures
Authors: Mei Zhe , Treado John D. , Grigas Alex T. , Levine Zachary A. , Regan Lynne , O'Hern Corey S. 
Published: February 2020

AbstractThere have been several studies suggesting that protein structures solved by NMR spectroscopy and X‐ray crystallography show significant differences. To understand the origin of these differences, we assembled a database of high‐quality protein structures solved by both methods. We also find significant differences between NMR and crystal structures—in the root‐mean‐square deviations of the C
α atomic positions, identities of core amino acids, backbone, and side‐chain dihedral angles, and packing fraction of core residues. In contrast to prior studies, we identify the physical basis for these differences by modeling protein cores as jammed packings of amino acid‐shaped particles. We find that we can tune the jammed packing fraction by varying the degree of thermalization used to generate the packings. For an athermal protocol, we find that the average jammed packing fraction is identical to that observed in the cores of protein structures solved by X‐ray crystallography. In contrast, highly thermalized packing‐generation protocols yield jammed packing fractions that are even higher than those observed in NMR structures. These results indicate that thermalized systems can pack more densely than athermal systems, which suggests a physical basis for the structural differences between protein structures solved by NMR and X‐ray crystallography.
Multi‐targets localization and elliptical circumnavigation by multi‐agents using bearing‐only measurements in two‐dimensional space
Authors: Chun Shaoheng , Tian Yu‐Ping 
Published: February 2020

SummaryIn order to enable the multi‐agents to elliptically circumnavigate the multi‐targets in more complex environment, we propose a geometric center estimator and an elliptical circumnavigation controller in two‐dimensional space by only employing bearing measurements without knowing the target's position and velocity. The stability of the algorithm is proved for both stationary targets and dynamic targets. Finally, a series of numerical simulations is presented to verify the correctness of the algorithm both in ideal networks and in networks with communication delays.
Carbon Quantum Dot Incorporated Nickel Pyrophosphate as Alternate Cathode for Supercapacitors
Authors: Priyadharshini Matheswaran , Pazhanivel Thangavelu , Bharathi Ganapathi 
Published: February 2020

AbstractIn spite of its large theoretical capacitance, the crystal structure of transition metal pyrophosphates frequently endures sluggish charge transfer and volume expansion leading to severe structural instability which may be ascribed to low power density and constant intercalation and deintercalation of ions in the matrix. Due to the stated facts, it resulted in quick capacity degradation and limited the overall performance of the energy device. To overcome the stated facts, we report the electrochemical charge storage performances of Nickel pyrophosphate/Carbon quantum dot (Ni2P2O7/CQD) nanocomposites. Here, we adopted a facile technique to prepare biowaste derived CQD and composited it with transition metal (Nickel) pyrophosphate. Incorporation of carbon quantum dots not only increased the specific capacitance than bare, besides, it also provides a long‐run cyclability. The addition of CQDs to the matrix favors rapid charge transfer and buffered the mechanical stress of nanocomposite. Using power law, the capacitance contribution from diffusion‐controlled and capacitive type mechanisms to the total specific capacitance was also elucidated. Hence the cost‐effective, facile synthesis method and favorable redox reaction empowered with voltage would have a potential application in high energy devices.
Preparation of rod‐like MgO by simple precipitation method for CO2 capture at ambient temperature
Authors: Tuan Vu Anh , Lee Chang Ha 
Published: February 2020

AbstractRod‐like MgO was prepared by the simple precipitation method and subsequently evaluated for CO2 capture efficiency under ambient‐temperature adsorption and intermediate‐temperature regeneration. The MgO precursor was analyzed by thermogravimetric analysis (TGA) and X‐ray power diffraction (XRD), it was decomposed completely at 500°C to form MgO. The as‐synthesized MgO samples were characterized by XRD, BET and FE‐SEM. The MgO samples were comprised of grains with an approximate length of 4‐6 μm and diameter of 0.5 μm. The BET surface area of MgO was 330.5 m2/g, which was much higher than that of commercial MgO (CM‐MgO, 103.2 m2/g). The CO2 uptake on MgO samples were evaluated by TGA. As‐synthesized MgO had the high CO2 sorption capacity compared to CM‐MgO. The effect of temperature on CO2 sorption capacity was also investigated. In addition, the stability of sample was evaluated by cyclic experiment.
Flavonoids from the leaves of Aralia dasyphylla Miq. (Araliaceae)
Authors: Thu Hien Nguyen Thi , Dieu Thuan Nguyen Thi , Van Huyen Pham , Anh Hong Truong Thi , Toan Phan Nguyen Huu 
Published: February 2020

AbstractFrom the methanol extract of leaves of Aralia dasyphylla Miq. collected in Da Lat, Lam Dong province, seven known compounds including kaempferol (1), quercetin (2), hispidulin (3), eupafolin (4), kaempferol‐7‐O‐α‐L‐rhamnoside (5), kaempferol 3,7‐O‐α‐L‐dirhamnopyranoside (6), kaempferol3‐O‐β‐D‐glucopyranoside‐7‐O‐α‐L‐rhamnoside (7) have been isolated for the first time from this plant by various chromatographic techniques. Structural elucidation of the metabolites was carried out by analysis of their spectroscopic data and comparison with those reported in the literature.
High‐purity amorphous silica from rice husk: Preparation and characterization
Authors: Ngoc Nguyen Nguyen , Thanh Le Xuan , Vinh La The , Van Anh Bui Thi 
Published: February 2020

AbstractAmorphous silica has a wide range of applications in chemical, biological and environmental sectors. In this work, we studied the preparation of amorphous silica from rice husk, an abundant silica‐rich agricultural waste in Vietnam. We investigated and compared two methods of preparation: (1) via selective removal of alkaline and alkaline‐earth oxides from rice husk ash to yield amorphous silica; and (2) via selective precipitation of silica from aqueous solutions which had been prepared by dissolving rice husk ask in NaOH solutions. The obtained silica was characterized by using XRD, EDX, SEM and BET techniques. The results show that the best obtained silica is a white amorphous and porous powder with high purity of SiO2, in the form of aggregates of 50 nm‐sized primary particles, and a specific surface area of 186 m2/g. The SiO2 recovery is around 8.3 (g) per 100 (g) rice husk. Moreover, in the context of the present work, method (1) can be a more straightforward route for the preparation of amorphous silica in terms of silica's purity, porousity and amorphousness. In contrast, method (2) is shown for a lower grade of silica purity which could be due to strong adsorption of ions from the solution onto the amorphous SiO2 precipitate during precipitation process. The obtained silica has promising applications as adsorbents, fillers and fertilizers.
Synthesis of reduced graphene oxide for high‐performance supercapacitor
Authors: Nam Pham Thi , Van Khanh Nguyen , Thom Nguyen Thi , Phuong Nguyen Thu , Van Trang Nguyen , Xuyen Nguyen Thi , Thai Vu Quoc , Tuan Vu Anh , Mai Thanh Dinh Thi 
Published: February 2020

AbstractReduced graphene oxide (rGO) were prepared by thermal reduction technique from graphene oxide (GO) in N2 gas atmosphere at 600°C for 1 h. The results shown that rGO possessed a multiple layer structure and a specific surface area BET of 389.9 m2/g. The electrochemical properties of the rGO materials have been investigated for supercapacitors. Electrochemical measurements show that rGO exhibit a specific capacitance of 85.3 F/g at 1 A/g, and still kept 85 % capacitance after 1000 cycles at 1 A/g, indicating rGO could be a promising candidate for high‐performance supercapacitor applications.
Theoretical investigation of the mechanism of indirect decomposition of nitrous oxide (N2O) by hydrogen (H2) on Cu7 cluster
Authors: Thuy Phan Thi , Thao Linh Dao Thi , Giang Vu Thi Huong , Cuong Ngo Tuan , Minh Hue Nguyen Thi 
Published: February 2020

AbstractMechanism of the decomposition reaction between N2O and H2 molecules on the Cu7 cluster was studied using density functional theory (DFT) with the BP86 functional in combination with the Aug‐cc‐pVDZ basis sets for N, O and H atoms, and the cc‐pVDZ‐PP basis set for Cu atom. The results show that the reaction undergoes via two successive steps. The first step involves adsorption of N2O on the Cu7 surface, followed by the decomposition of N2O with an energy barrier of barely ~3 kcal/mol, producing an O atom, which subsequently binds to the cluster and a N2 molecule. In the second step, the adsorped O atom couples with the H2 molecule bound to a different Cu atom of the cluster leading to a release of H2O molecule via two different pathways. The pathway corresponding to an adsorption of a N2O molecule onto the most positively charged site of Cu7 as turns out that the most energetically favorable with an energy barrier of ~22 kcal/mol, which is much lower than the barrier of 42 kcal/mol for the reaction without a catalyst.
Effect of calcination temperature on phase evolution and photoluminescent properties of GdPO4:Eu3+ nanoparticle phosphors synthesized by combustion method
Authors: Dieu Hien Thai Thi , Roan Pham Duc , Thanh Nguyen Trong , Tien Dinh Manh , Vu Nguyen 
Published: February 2020

AbstractEu3+ ‐doped GdPO4 nanoparticles were synthesized by a straightforward combustion method using urea as fuel and metal nitrates as precursors. Structures, morphologies and photoluminescent properties of the synthesized samples were studied by X‐ray diffraction, scanning electron microscopy and photoluminescent spectroscopy. There are two types of crystal structures formed during the annealing process: hexagonal GdPO4.H2O and monoclinic GdPO4. The former can be found at low annealing temperatures (300‐500°C) while the latter can be obtained at higher annealing temperatures (700‐900°C). The average diameters for nanoparticles are 20‐30 nm. Under the excitation at 273 nm, both GdPO4.H2O:Eu3+ and GdPO4:Eu3+ powders showed red emission bands originating from the 5D0 ‐ 7FJ (J = 1, 2, 3 and 4) transitions of Eu3+. Their PL intensity and crystal structures exhibit a similar behavior as a function of the annealing temperature.
Influence of ammonia on properties of TiO2‐MgFe2O4 as high visible‐light active photocatalysts for the degradation of Rhodamine B
Authors: Nha Khanh Dang Nguyen , Lin Hoang Ngoc , Mai Tho Nguyen Thi , Nhat Ha Ho Nguyen , Huy Vu Quang , Phuong Dieu Nguyen Thi , Huy Do Manh , Dat Duong Phuoc , Kim Phuong Nguyen Thi 
Published: February 2020

AbstractThe influence of ammonia on TiO2‐MgFe2O4 catalysts synthesized via a hydrolysis and co‐precipitation, followed by calcination at 500°C, was studied. Two different catalysts, TiO2‐MgFe2O4 (Am) and TiO2‐MgFe2O4 (W), were prepared using Ti‐precursors, which were synthesized by the hydrolysis of Ti‐butoxide with and without ammonia. The resulting TiO2‐MgFe2O4 catalysts were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), specific surface area measurements, X‐ray diffraction, and UV‐vis diffuse reflectance techniques. It was revealed that strong electronic coupling exists between the TiO2 and MgFe2O4 components within the TiO2‐MgFe2O4 catalysts. Photocatalytic activity toward Rhodamine B (RhB) was investigated in aqueous solution under visible light irradiation. TiO2‐MgFe2O4 (W) was found to be an effective catalyst and had several advantages over TiO2‐MgFe2O4 (Am). These results clearly highlight that ammonia had a significant influence on the photocatalytic activity of the TiO2‐MgFe2O4 catalysts. Therefore, the results reported herein indicate that TiO2‐MgFe2O4 is a green, low‐cost, and highly efficient photocatalyst for environmental remediation.
Study on water soluble constituentsfrom Gomphrena celoisiodes
Authors: Trang Do Thi , Tai Bui Huu , Yen Pham Hai , Yen Duong Thi Hai , Nhiem Nguyen Xuan , Kiem Phan Van 
Published: February 2020

AbstractUsing combined chromatographic methods, eight glycoside compounds including two flavones: isorhamnetin 3‐O‐α‐L‐rhamnopyranosyl‐(1→6)‐β‐D‐glucopyranoside (1), kaempferol 3‐O‐β‐D‐glucopyranoside (2), three phenyls: 2‐phenylethyl β‐rutinoside (3), 2‐phenylethyl β‐primeveroside (4), hydrangeifolin I (5); two lignans: tortoside A (6), pinoresinol‐4′′‐O‐β‐D‐glucopyranoside (7), and a megastigmaneicariside B2 (8) were isolated from the methanol extract of the aerial part of Gomphrena celoisiodes. Their structures were identified on the basis of spectroscopic evidence and in comparison with those reported in the literature. All these compounds were isolated from G. celoisiodes for the first time.
Synthesis of mesoporous SiO2 from rice husk for removal of organic dyes in aqueous solution
Authors: Thu Hoang Thi , Dat Le Tien , Tuan Vu Anh 
Published: February 2020

AbstractMesoporous SiO2 was prepared from rice husk for removal of methylene blue (MB) from aqueous solution. As‐prepared SiO2 was characterized by FE‐SEM, TEM, XRD, FT‐IR and N2 adsorption/desorption isotherm. The effects of SiO2 dosage and contact time on adsorption of dye were investigated. The optimal dosage of SiO2 for removing methylene blue was 0.05 g and the removal efficiency of methylene blue reached to 91.9 % after 50 min of adsorption. The adsorption kinetic of methylene blue was studied to understand the mechanism of adsorption process. The kinetic data was analyzed by pseudo‐first‐order, pseudo‐second‐order, and intra‐particle diffusion models. The results showed that rate of adsorption dye followed pseudo‐second‐model with a good regression correlation (R2 = 0.999), the value of rate constant for adsorption process was calculated as 0.017 (g.mg‐1.min‐1) and film diffusion was the rate limiting step. In addition, the cyclic test for as‐prepared SiO2 was investigated and the removal efficiency was remained at 80.2% at 3rd cyclic experiment.
The chemical constituents and biological activity of some sponges in Northern Vietnam: A review
Authors: Van Kiem Phan , Van Minh Chau , Nhiem Nguyen Xuan , Tai Bui Huu , Yen Pham Hai , Hang Dan Thi Thuy , Dung Duong Thi , Cuc Nguyen Thi , Trang Do Thi , Yen Duong Thi Hai , Van Doan Vu , Hoang Nguyen Huy , Huong Phan Thi Thanh , Nam Nguyen Hoai , Cuong Nguyen Xuan 
Published: February 2020

AbstractChemical composition and biological activity of sponges have been studied by the authors since 2006. So far, the authors have studied about 30 species of sponges, mainly collected cross in the sea of Northern Vietnam. Over 250 compounds have been isolated and elucidated their chemical structures. Among them, there are more than 50 compounds with new chemical structures. In addition to NMR and HRMS spectra, many compounds with an unequal chemical structure are also determined by Circular Dichroism spectrometry. Some isolated compounds exhibited outstanding potential for cancer cytotoxicity and anti‐inflammatory effects.
1H‐Indole‐3‐acetonitrile glycoside, phenolic and other compounds from Stixis suaveolens
Authors: Anh Ngo Quoc , Yen Tran Thi , Hang Nguyen Thuy , Anh Duong Hong , Viet Pham Hung , Van Doan Vu , Van Kiem Phan 
Published: February 2020

AbstractFrom the methanol extract of the Stixis suaveolensleaves, 1H‐Indole‐3‐acetonitrile4‐O‐β‐D‐glucopyranoside (1), α‐adenosine (2), β‐adenosine (3), 1‐O‐syrinoyl‐β‐D‐glucopyranoside (4), 2,6‐dimethoxy‐p‐hydroquinone 1‐O‐β‐D‐glucopyranoside(5), icariside B5 (6) were isolated by various chromatographic methods. The chemical structures of these compounds were elucidated by HRESI‐MS, 1D and 2D NMR spectra and by comparison with data reported in the literature. This is the first report of these compounds from Stixis suaveolens.
Isolation and characterization of six flavonoids from the leaves of Sterculia foetida Linn.
Authors: Cuong Doan Tran Duy , Dat Ha Tuan , Duan Nguyen Trung , Thuong Pham Dinh , Phat Nguyen Tan , Tri Mai Dinh , Van Son Dang , Hoa Nguyen Thi , Tuyen Pham Nguyen Kim , Phung Nguyen Kim Phi 
Published: February 2020

AbstractSix flavonoids, including one flavan‐3‐ol epicatechin (1), one flavone glycoside apigenin 7‐O‐β‐D‐glucopyranoside (2), three flavones namely dimethylchrysin (3), trimethylapigenin (4) and luteolin (5) and one flavonol quercetin (6) were isolated from the hexane and chloroform extracts of the leaves of Sterculia foetida Linn. collected in Binh Thuan Province by using various chromatographic methods. Their chemical structures were identified by comparing their spectroscopic data as well as physical properties with those in literature. Compounds 1‐4 were obtained for the first time in Sterculia genus.