bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reducing consumption of unhealthy foods and beverages through banning price promotions: what is the evidence and will it work?
Authors: Watt Toby LS , Beckert Walter , Smith Richard D , Cornelsen Laura 
Published: May 2020

AbstractObjective:Increasing prevalence of overweight and obese people in England has led policymakers to consider regulating the use of price promotions on foods high in fat, sugar and salt content. In January 2019, the government opened a consultation programme for a policy proposal that significantly restricts the use of price promotions that can induce consumers to buy higher volumes of unhealthy foods and beverages. These proposed policies are the first of their kind in public health and are believed to reduce excess purchasing and, therefore, overconsumption of unhealthy products. This study summarises evidence relating price promotions to the purchasing of food and drink for home consumption and places it in the context of the proposed policy.Design:Non-systematic review of quantitative analyses of price promotions in food and drink published in peer-reviewed journals and sighted by PubMed, ScienceDirect & EBSCOhost between 1980 and January 2018.Results:While the impact of price promotions on sales has been of interest to marketing academics for a long time with modelling studies showing that its use has increased food and drink sales by 12–43 %, it is only now being picked up in the public health sphere. However, existing evidence does not consider the effects of removing or restricting the use of price promotions across the food sector. In this commentary, we discuss existing evidence, how it deals with the complexity of shoppers’ behaviour in reacting to price promotions on foods and, importantly, what can be learned from it in this policy context.Conclusions:The current evidence base supports the notion that price promotions increase purchasing of unhealthy food, and while the proposed restriction policy is yet to be evaluated for consumption and health effects, there is arguably sufficient evidence to proceed. This evidence is not restricted to volume-based promotions. Close monitoring and proper evaluation should follow to provide empirical evidence of its intended and unintended effects.
Evaluation of Particle Size Distribution Metrics to Estimate the Relative Contributions of Different Size Fractions Based on Measurements in Arctic Waters
Authors: Runyan Hugh , Reynolds Rick A. , Stramski Dariusz 
Published: May 2020

AbstractThe size distribution of suspended particles influences several processes in aquatic ecosystems, including light propagation, trophic interactions, and biogeochemical cycling. The shape of the particle size distribution (PSD) is commonly modeled as a single‐slope power law in oceanographic studies, which can be used to further estimate the relative contributions of different particle size classes to particle number, area, and volume concentration. We use a data set of 168 high size‐resolution PSD measurements in Arctic oceanic waters to examine variability in the shape of the PSD over the particle diameter range 0.8 to 120 μm. An average value of −3.6 ± 0.33 was obtained for the slope of a power law fitted over this size range, consistent with other studies. Our analysis indicates, however, that this model has significant limitations in adequately parameterizing the complexity of the PSD, and thus performs poorly in predicting the relative contributions of different size intervals such as those based on picoplankton, nanoplankton, and microplankton size classes. Similarly, median particle size was also generally a poor indicator of these size class contributions. Our results suggest that alternative percentile diameters derived from the cumulative distribution functions of particle number, cross‐sectional area, and volume concentration may provide better metrics to capture the overall shape of the PSD and to quantify the contributions of different particle size classes.
Selective carbon sources influence the end products of microbial nitrate respiration
Authors: Carlson Hans K , Lui Lauren M , Price Morgan N , Kazakov Alexey E , Carr Alex V , Kuehl Jennifer V , Owens Trenton K , Nielsen Torben , Arkin Adam P , Deutschbauer Adam M 
Published: May 2020

Abstract
Respiratory and catabolic genes are differentially distributed across microbial genomes. Thus, specific carbon sources may favor different respiratory processes. We profiled the influence of 94 carbon sources on the end products of nitrate respiration in microbial enrichment cultures from diverse terrestrial environments. We found that some carbon sources consistently favor dissimilatory nitrate reduction to ammonium (DNRA/nitrate ammonification) while other carbon sources favor nitrite accumulation or denitrification. For an enrichment culture from aquatic sediment, we sequenced the genomes of the most abundant strains, matched these genomes to 16S rDNA exact sequence variants (ESVs), and used 16S rDNA amplicon sequencing to track the differential enrichment of functionally distinct ESVs on different carbon sources. We found that changes in the abundances of strains with different genetic potentials for nitrite accumulation, DNRA or denitrification were correlated with the nitrite or ammonium concentrations in the enrichment cultures recovered on different carbon sources. Specifically, we found that either L-sorbose or D-cellobiose enriched for a Klebsiella nitrite accumulator, other sugars enriched for an Escherichia nitrate ammonifier, and citrate or formate enriched for a Pseudomonas denitrifier and a Sulfurospirillum nitrate ammonifier. Our results add important nuance to the current paradigm that higher concentrations of carbon will always favor DNRA over denitrification or nitrite accumulation, and we propose that, in some cases, carbon composition can be as important as carbon concentration in determining nitrate respiratory end products. Furthermore, our approach can be extended to other environments and metabolisms to characterize how selective parameters influence microbial community composition, gene content, and function.
Development of a new macrophage-specific TRAP mouse (MacTRAP) and definition of the renal macrophage translational signature
Authors: Hofmeister Andreas , Thomaßen Maximilian C. , Markert Sabrina , Marquardt André , Preußner Mathieu , Rußwurm Martin , Schermuly Ralph T. , Steinhoff Ulrich , Gröne Hermann-Josef , Hoyer Joachim , Humphreys Benjamin D. , Grgic Ivica 
Published: May 2020

AbstractTissue macrophages play an important role in organ homeostasis, immunity and the pathogenesis of various inflammation-driven diseases. One major challenge has been to selectively study resident macrophages in highly heterogeneous organs such as kidney. To address this problem, we adopted a Translational Ribosome Affinity Purification (TRAP)- approach and designed a transgene that expresses an eGFP-tagged ribosomal protein (L10a) under the control of the macrophage-specific c-fms promoter to generate c-fms-eGFP-L10a transgenic mice (MacTRAP). Rigorous characterization found no gross abnormalities in MacTRAP mice and confirmed transgene expression across various organs. Immunohistological analyses of MacTRAP kidneys identified eGFP-L10a expressing cells in the tubulointerstitial compartment which stained positive for macrophage marker F4/80. Inflammatory challenge led to robust eGFP-L10a upregulation in kidney, confirming MacTRAP responsiveness in vivo. We successfully extracted macrophage-specific polysomal RNA from MacTRAP kidneys and conducted RNA sequencing followed by bioinformatical analyses, hereby establishing a comprehensive and unique in vivo gene expression and pathway signature of resident renal macrophages. In summary, we created, validated and applied a new, responsive macrophage-specific TRAP mouse line, defining the translational profile of renal macrophages and dendritic cells. This new tool may be of great value for the study of macrophage biology in different organs and various models of injury and disease.
Ab Initio Prediction of the Phase Transition for Solid Ammonia at High Pressures
Authors: Huang Lei , Han Yanqiang , Liu Jinyun , He Xiao , Li Jinjin 
Published: May 2020

AbstractAmmonia is one of the most basic components on the planet and its high-pressure characteristics play an important role in planetary science. Solid ammonia crystals frequently adopt multiple distinct polymorphs exhibiting different properties. Predicting the crystal structure of these polymorphs and under what thermodynamic conditions these polymorphs are stable would be of great value to environmental industry and other fields. Theoretical calculations based on the classical force fields and density-functional theory (DFT) are versatile methods but lack of accurate description of weak intermolecular interactions for molecular crystals. In this study, we employ an ab initio computational study on the solid ammonia at high pressures, using the second-order Møller-Plesset perturbation (MP2) theory and the coupled cluster singles, doubles, and perturbative triples (CCSD(T)) theory along with the embedded fragmentation method. The proposed algorithm is capable of performing large-scale calculations using high-level wavefunction theories, and accurately describing covalent, ionic, hydrogen bonding, and dispersion interactions within molecular crystals, and therefore can predict the crystal structures, Raman spectra and phase transition of solid ammonia phases I and IV accurately. We confirm the crystal structures of solid ammonia phases I and IV that have been controversial for a long time and predict their phase transition that occurs at 1.17 GPa and 210 K with small temperature dependence, which is in line with experiment.
Validation of a mitochondrial RNA therapeutic strategy using fibroblasts from a Leigh syndrome patient with a mutation in the mitochondrial ND3 gene
Authors: Yamada Yuma , Somiya Kana , Miyauchi Akihiko , Osaka Hitoshi , Harashima Hideyoshi 
Published: May 2020

AbstractWe report on the validation of a mitochondrial gene therapeutic strategy using fibroblasts from a Leigh syndrome patient by the mitochondrial delivery of therapeutic mRNA. The treatment involves delivering normal ND3 protein-encoding mRNA as a therapeutic RNA to mitochondria of the fibroblasts from a patient with a T10158C mutation in the mtDNA coding the ND3 protein, a component of the mitochondrial respiratory chain complex I. The treatment involved the use of a liposome-based carrier (a MITO-Porter) for delivering therapeutic RNA to mitochondria via membrane fusion. The results confirmed that the mitochondrial transfection of therapeutic RNA by the MITO-Porter system resulted in a decrease in the levels of mutant RNA in mitochondria of diseased cells based on reverse transcription quantitative PCR. An evaluation of mitochondrial respiratory activity by respirometry also showed that transfection using the MITO-Porter resulted in an increase in maximal mitochondrial respiratory activity in the diseased cells.
Enhanced Cerebral Blood Volume under Normobaric Hyperoxia in the J20-hAPP Mouse Model of Alzheimer’s Disease
Authors: Shabir Osman , Sharp Paul , Rebollar Monica A. , Boorman Luke , Howarth Clare , Wharton Stephen B. , Francis Sheila E. , Berwick Jason 
Published: May 2020

AbstractEarly impairments to neurovascular coupling have been proposed to be a key pathogenic factor in the onset and progression of Alzheimer’s disease (AD). Studies have shown impaired neurovascular function in several mouse models of AD, including the J20-hAPP mouse. In this study, we aimed to investigate early neurovascular changes using wild-type (WT) controls and J20-hAPP mice at 6 months of age, by measuring cerebral haemodynamics and neural activity to physiological sensory stimulations. A thinned cranial window was prepared to allow access to cortical vasculature and imaged using 2D-optical imaging spectroscopy (2D-OIS). After chronic imaging sessions where the skull was intact, a terminal acute imaging session was performed where an electrode was inserted into the brain to record simultaneous neural activity. We found that cerebral haemodynamic changes were significantly enhanced in J20-hAPP mice compared with controls in response to physiological stimulations, potentially due to the significantly higher neural activity (hyperexcitability) seen in the J20-hAPP mice. Thus, neurovascular coupling remained preserved under a chronic imaging preparation. Further, under hyperoxia, the baseline blood volume and saturation of all vascular compartments in the brains of J20-hAPP mice were substantially enhanced compared to WT controls, but this effect disappeared under normoxic conditions. This study highlights novel findings not previously seen in the J20-hAPP mouse model, and may point towards a potential therapeutic strategy.
Historic recombination in a durum wheat breeding panel enables high-resolution mapping of Fusarium head blight resistance quantitative trait loci
Authors: Sari Ehsan , Knox Ron E. , Ruan Yuefeng , Henriquez Maria Antonia , Kumar Santosh , Burt Andrew J. , Cuthbert Richard D. , Konkin David J. , Walkowiak Sean , Campbell Heather L. , Singh Asheesh K. , Ross Jay , Lokuruge Prabhath , Hsueh Emma , Boyle Kerry , Sidebottom Christine , Condie Janet , Yates Shawn , Pozniak Curtis J. , Fobert Pierre R. 
Published: May 2020

AbstractThe durum wheat line DT696 is a source of moderate Fusarium head blight (FHB) resistance. Previous analysis using a bi-parental population identified two FHB resistance quantitative trait loci (QTL) on chromosome 5A: 5A1 was co-located with a plant height QTL, and 5A2 with a major maturity QTL. A Genome-Wide Association Study (GWAS) of DT696 derivative lines from 72 crosses based on multi-environment FHB resistance, plant height, and maturity phenotypic data was conducted to improve the mapping resolution and further elucidate the genetic relationship of height and maturity with FHB resistance. The Global Tetraploid Wheat Collection (GTWC) was exploited to identify durum wheat lines with DT696 allele and additional recombination events. The 5A2 QTL was confirmed in the derivatives, suggesting the expression stability of the 5A2 QTL in various genetic backgrounds. The GWAS led to an improved mapping resolution rendering the 5A2 interval 10 Mbp shorter than the bi-parental QTL mapping interval. Haplotype analysis using SNPs within the 5A2 QTL applied to the GTWC identified novel haplotypes and recombination breakpoints, which could be exploited for further improvement of the mapping resolution. This study suggested that GWAS of derivative breeding lines is a credible strategy for improving mapping resolution.
Neural evidence that disengaging memory retrieval is modulated by stimulus valence and rumination
Authors: Xia Jiangyi , Evans Lisa H. 
Published: May 2020

AbstractTo remember information from our personal past we need to be in a cognitive state where we treat stimuli as cues for memory retrieval. In this study we considered whether participants could exert control and disengage from a memory state when it was no longer required for the task at hand. In particular, we examined whether this ability was affected by the valence of the stimuli and participant’s rumination scores. After a study phase participants completed test blocks where the task switched every two trials between a memory task (retrieving information from the study phase) and a perceptual task. Even though there was no episodic memory requirement in the perceptual task, a well-established event-related potential (ERP) index of memory retrieval was present for both trials when the stimuli were negative valenced pictures but not for neutral pictures. Furthermore, there was a positive correlation between the magnitude of this ERP memory index in the perceptual task and rumination scores but only for neutral stimuli and not negative. Thus, in this study participants generally had difficultly suppressing memory retrieval when negative stimuli were presented. However, for neutral stimuli only ruminators were more susceptible to memory intrusions.
First demonstration of coherent resonant backward diffraction radiation for a quasi-monochromatic terahertz-light source
Authors: Sei Norihiro , Takahashi Toshiharu 
Published: May 2020

AbstractWe proposed coherent resonant backward diffraction radiation (CRBDR), which generates wavelength-tunable quasi-monochromatic lights using a compact diffractor assembly in an accelerator facility of high-energy electron beams, as a unique intense terahertz (THz) light source. Superimposing the coherent backward diffracted radiation emitted by periodically arranged hollow diffractors, it is possible to amplify the frequency components satisfying a resonant condition, and make the radiation monochromatic. We demonstrated the CRBDR using the L-band linac at the Institute for Integrated Radiation and Nuclear Science at Kyoto University. It was observed that the coherent backward diffraction radiation was amplified more than three times at a frequency which was the fundamental resonant frequency in the CRBDR theory. Moreover, the number of diffractors at the saturation of the radiation power was consistent with the number estimated from the electron distribution in a bunch. The experimental results show that the CRBDR is useful as a quasi-monochromatic light source in the THz band.
Extrahepatic cholangiocyte obstruction is mediated by decreased glutathione, Wnt and Notch signaling pathways in a toxic model of biliary atresia
Authors: Fried Sophia , Gilboa Dafna , Har-Zahav Adi , Lavrut Pierre-Marie , Du Yu , Karjoo Sara , Russo Pierre , Shamir Raanan , Wells Rebecca G. , Waisbourd-Zinman Orith 
Published: May 2020

AbstractBiliary atresia is a neonatal liver disease with extrahepatic bile duct obstruction and progressive liver fibrosis. The etiology and pathogenesis of the disease are unknown. We previously identified a plant toxin, biliatresone, responsible for biliary atresia in naturally-occurring animal models, that causes cholangiocyte destruction inin-vitromodels. Decreases in reduced glutathione (GSH) mimic the effects of biliatresone, and agents that replenish cellular GSH ameliorate the effects of the toxin. The goals of this study were to define signaling pathways downstream of biliatresone that lead to cholangiocyte destruction and to determine their relationship to GSH. Using cholangiocyte culture and 3D cholangiocyte spheroid cultures, we found that biliatresone and decreases in GSH upregulated RhoU/Wrch1, a Wnt signaling family member, which then mediated an increase in Hey2 in the NOTCH signaling pathway, causing downregulation of the transcription factor Sox17. When these genes were up- or down-regulated, the biliatresone effect on spheroids was phenocopied, resulting in lumen obstruction. Biopsies of patients with biliary atresia demonstrated increased RhoU/Wrch1 and Hey2 expression in cholangiocytes. We present a novel pathway of cholangiocyte injury in a model of biliary atresia, which is relevant to human BA and may suggest potential future therapeutics.
Foraging strategy of a carnivorous-insectivorous raptor species based on prey size, capturability and nutritional components
Authors: Fargallo Juan A. , Navarro-López Juan , Palma-Granados Patricia , Nieto Rosa M. 
Published: May 2020

AbstractOptimal foraging theory has typically paid little attention to species feeding on mobile prey and has emphasised energy intake rather than the nutritional contribution of food. The difficulty of capturing food has rarely been included in foraging models, even when it is a potentially important modulator of time devoted to foraging. From the central place foraging and provisioning perspectives, it is posited that at high levels of prey selectivity, the time spent to capture prey is longer than at low levels of prey selectivity. Furthermore, in the case of carnivorous predators, it is thought that nutritional composition does not influence foraging strategies. To explore these issues, we investigated the influence of abundance, size, difficulty of capture, gross energy and nutritional composition (fat, protein, protein-fat ratio and amino acid contents) of prey species on the foraging behaviour of a predator species, the common kestrelFalco tinnunculus, in a region of high diversity of prey species. Our results show that capturability index and load-size explain the foraging behaviour of kestrels. Preferred prey take longer to be provisioned, both selectivity and capturability might explain this result. It is also shown that specific nutritional components, such as protein and amino acid contents, are likely to explain food preference in this carnivorous-insectivorous species.
Human arylamine N-acetyltransferase 2 genotype-dependent protein expression in cryopreserved human hepatocytes
Authors: Salazar-González Raúl A. , Doll Mark A. , Hein David W. 
Published: May 2020

AbstractHuman N-acetyltransferases (NAT; EC 2.3.1.5) catalyze the N-acetylation of arylamine and hydrazine drugs and the O-acetylation of N-hydroxylated metabolites of aromatic and heterocyclic amines. Two different isoforms of this protein, N-acetyltransferase 1 (NAT1) and N-acetyltransferase 2 (NAT2), are expressed in human hepatocytes. Both are encoded by a single 870-bp open reading frame that exhibits genetic polymorphisms in human populations. NAT1 and NAT2 share more than 85% gene and protein sequence, making it challenging to produce antibodies with high specificity for NAT1 or NAT2. In the present study, we compared methods for the quantification of immunoreactive NAT1 and NAT2 with seven different antibodies and investigated the relationship of NAT2 genotype to NAT2 mRNA and protein expression in cryopreserved human hepatocytes. Sulfamethazine (NAT2-selective substrate) and NAT2 protein expression differed significantly with NAT2 acetylator genotype (p < 0.0001). NAT2 protein expression and sulfamethazine NAT2 catalytic activity correlated highly across the cryopreserved human hepatocytes of rapid, intermediate, and slow acetylator NAT2 genotypes. In conclusion, our data describe a specific analytical method for the quantification of NAT1 and NAT2 protein expression. We showed that the NAT2 activity in human hepatocytes is directly correlated to expression levels of NAT2 protein but not mRNA.
Hybrid image sensor of small molecule organic photodiode on CMOS – Integration and characterization
Authors: Shekhar Himanshu , Fenigstein Amos , Leitner Tomer , Lavi Becky , Veinger Dmitry , Tessler Nir 
Published: May 2020

AbstractOrganic photodiodes (OPDs) for its interesting optoelectronic properties has the potential to be utilized with complementary metal-oxide-semiconductor (CMOS) circuit for imaging, automotive, and security based applications. To achieve such a hybrid device as an image sensor, it is imperative that the quality of the OPD remains high on the CMOS substrate and that it has a well-connected optoelectronic interface with the underneath readout integrated circuit (ROIC) for efficient photogeneration and signal readout. Here, we demonstrate seamless integration of a thermally deposited visible light sensitive small molecule OPD on a standard commercial CMOS substrate using optimized doped PCBM buffer layer. Under a standard power supply voltage of 3 V, this hybrid device shows an excellent photolinearity in the entire bias regime, a high pixel sensitivity of 2 V/Lux.sec, a dynamic range (DR) of 71 dB, and a low dark leakage current density of 1 nA/cm2. Moreover, the integrated OPD has a minimum bandwidth of 400 kHz. The photoresponse nonuniformity being only 1.7%, achieved under research lab conditions, strengthens the notion that this fully-CMOS compatible technology has the potential to be applied in high-performance large-scale imaging array.