bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased airway iron parameters and risk for exacerbation in COPD: an analysis from SPIROMICS
Authors: Zhang William Z. , Oromendia Clara , Kikkers Sarah Ann , Butler James J. , O’Beirne Sarah , Kim Kihwan , O’Neal Wanda K. , Freeman Christine M. , Christenson Stephanie A. , Peters Stephen P. , Wells J. Michael , Doerschuk Claire , Putcha Nirupama , Barjaktarevic Igor , Woodruff Prescott G. , Cooper Christopher B. , Bowler Russell P. , Comellas Alejandro P. , Criner Gerard J. , Paine Robert , Hansel Nadia N. , Han Meilan K. , Crystal Ronald G. , Kaner Robert J. , Ballman Karla V. , Curtis Jeffrey L. , Martinez Fernando J. , Cloonan Suzanne M. 
Published: June 2020

AbstractLevels of iron and iron-related proteins including ferritin are higher in the lung tissue and lavage fluid of individuals with chronic obstructive pulmonary disease (COPD), when compared to healthy controls. Whether more iron in the extracellular milieu of the lung associates with distinct clinical phenotypes of COPD, including increased exacerbation susceptibility, is unknown. We measured iron and ferritin levels in the bronchoalveolar lavage fluid (BALF) of participants enrolled in the SubPopulations and InteRmediate Outcome Measures In COPD (SPIROMICS) bronchoscopy sub-study (n = 195). BALF Iron parameters were compared to systemic markers of iron availability and tested for association with FEV1 % predicted and exacerbation frequency. Exacerbations were modelled using a zero-inflated negative binomial model using age, sex, smoking, and FEV1 % predicted as clinical covariates. BALF iron and ferritin were higher in participants with COPD and in smokers without COPD when compared to non-smoker control participants but did not correlate with systemic iron markers. BALF ferritin and iron were elevated in participants who had COPD exacerbations, with a 2-fold increase in BALF ferritin and iron conveying a 24% and 2-fold increase in exacerbation risk, respectively. Similar associations were not observed with plasma ferritin. Increased airway iron levels may be representative of a distinct pathobiological phenomenon that results in more frequent COPD exacerbation events, contributing to disease progression in these individuals.
AAV8 Ins1-Cre can produce efficient β-cell recombination but requires consideration of off-target effects
Authors: Ramzy Adam , Tudurí Eva , Glavas Maria M. , Baker Robert K. , Mojibian Majid , Fox Jessica K. , O’Dwyer Shannon M. , Dai Derek , Hu Xiaoke , Denroche Heather C. , Edeer Nazde , Gray Sarah L. , Verchere Cameron B. , Johnson James D. , Kieffer Timothy J. 
Published: June 2020

AbstractIn vivo genetic manipulation is used to study the impact of gene deletion or re-expression on β-cell function and organism physiology. Cre-LoxP is a system wherein LoxP sites flanking a gene are recognized by Cre recombinase. Cre transgenic mice are the most prevalent technology used to deliver Cre but many models have caveats of off-target recombination, impaired β-cell function, and high cost of animal production. Inducible estrogen receptor conjugated Cre models face leaky recombination and confounding effects of tamoxifen. As an alternative, we characterize an adeno associated virus (AAV) with a rat insulin 1 promoter driving Cre recombinase (AAV8 Ins1-Cre) that is economical and rapid to implement, and has limited caveats. Intraperitoneal AAV8 Ins1-Cre produced efficient β-cell recombination, alongside some hepatic, exocrine pancreas, α-cell, δ-cell, and hypothalamic recombination. Delivery of lower doses via the pancreatic duct retained good rates of β-cell recombination and limited rates of off-target recombination. Unlike inducible Cre in transgenic mice, AAV8 Ins1-Cre required no tamoxifen and premature recombination was avoided. We demonstrate the utility of this technology by inducing hyperglycemia in inducible insulin knockout mice (Ins1−/−;Ins2f/f). AAV-mediated expression of Cre in β-cells provides an effective alternative to transgenic approaches for inducible knockout studies.
First demonstration of machine-designed ultra-flat, low-cost directive antenna
Authors: Zucchi Marcello , Giordanengo Giorgio , Righero Marco , Vecchi Giuseppe 
Published: June 2020

AbstractIn this paper, we present a fully automated procedure for the direct design of a novel class of single-feed flat antennas with patterning of a conductive surface. We introduce a convenient surface discretization, based on hexagonal cells, and define an appropriate objective function, including both gain and input matching requirements. The reference geometry is constituted by a very thin, single feed-point square panel. It features a backing metal plate (“ground”) and a top conductive layer, which is automatically patterned to achieve the desired radiation and input matching properties. The process employs an evolutionary algorithm combined with a boundary element electromagnetic solver. By applying this method, we designed an antenna tailored to the 2.4 GHz ISM frequency band, with a size of $$24\,\hbox {cm} \times 24\,\hbox {cm}$$24cm×24cm, i.e., $$2 \times 2$$2×2 wavelengths and an height of 4 mm, or 0.03 wavelengths. Measured data confirmed the expected high gain (13 dBi), with a remarkable aperture efficiency (higher than 50%, including losses), thus validating the proposed approach.
Development of fluorescent Escherichia coli for a whole-cell sensor of 2ʹ-fucosyllactose
Authors: Shin Jonghyeok , Park Myungseo , Kim Chakhee , Kim Hooyeon , Park Yunjeong , Ban Choongjin , Yoon Jong-Won , Shin Chul-Soo , Lee Jae Won , Jin Yong-Su , Park Yong-Cheol , Min Won-Ki , Kweon Dae-Hyuk 
Published: June 2020

Abstract2′-Fucosyllactose (2′-FL), a major component of fucosylated human milk oligosaccharides, is beneficial to human health in various ways like prebiotic effect, protection from pathogens, anti-inflammatory activity and reduction of the risk of neurodegeneration. Here, a whole-cell fluorescence biosensor for 2′-FL was developed. Escherichia coli (E. coli) was engineered to catalyse the cleavage of 2′-FL into l-fucose and lactose by constitutively expressing α-l-fucosidase. Escherichia coli ∆L YA, in which lacZ is deleted and lacY is retained, was employed to disable lactose consumption. E. coli ∆L YA constitutively co-expressing α-l-fucosidase and a red fluorescence protein (RFP) exhibited increased fluorescence intensity in media containing 2′-FL. However, the presence of 50 g/L lactose reduced the RFP intensity due to lactose-induced cytotoxicity. Preadaptation of bacterial strains to fucose alleviated growth hindrance by lactose and partially recovered the fluorescence intensity. The fluorescence intensity of the cell was linearly proportional to 1–5 g/L 2′-FL. The whole-cell sensor will be versatile in developing a 2′-FL detection system.
The longest path in the Price model
Authors: Evans Tim S. , Calmon Lucille , Vasiliauskaite Vaiva 
Published: June 2020

AbstractThe Price model, the directed version of the Barabási–Albert model, produces a growing directed acyclic graph. We look at variants of the model in which directed edges are added to the new vertex in one of two ways: using cumulative advantage (preferential attachment) choosing vertices in proportion to their degree, or with random attachment in which vertices are chosen uniformly at random. In such networks, the longest path is well defined and in some cases is known to be a better approximation to geodesics than the shortest path. We define a reverse greedy path and show both analytically and numerically that this scales with the logarithm of the size of the network with a coefficient given by the number of edges added using random attachment. This is a lower bound on the length of the longest path to any given vertex and we show numerically that the longest path also scales with the logarithm of the size of the network but with a larger coefficient that has some weak dependence on the parameters of the model.
Elevated glucose is associated with hemorrhagic transformation after mechanical thrombectomy in acute ischemic stroke patients with severe pretreatment hypoperfusion
Authors: Laredo Carlos , Renú Arturo , Llull Laura , Tudela Raúl , López-Rueda Antonio , Urra Xabier , Macías Napoleón G. , Rudilosso Salvatore , Obach Víctor , Amaro Sergio , Chamorro Ángel 
Published: June 2020

AbstractSeveral pretreatment variables such as elevated glucose and hypoperfusion severity are related to brain hemorrhage after endovascular treatment of acute stroke. We evaluated whether elevated glucose and severe hypoperfusion have synergistic effects in the promotion of parenchymal hemorrhage (PH) after mechanical thrombectomy (MT). We included 258 patients MT-treated who had a pretreatment computed tomography perfusion (CTP) and a post-treatment follow-up MRI. Severe hypoperfusion was defined as regions with cerebral blood volume (CBV) values < 2.5% of normal brain [very-low CBV (VLCBV)-regions]. Median baseline glucose levels were 119 (IQR = 105–141) mg/dL. Thirty-nine (15%) patients had pretreatment VLCBV-regions, and 42 (16%) developed a PH after MT. In adjusted models, pretreatment glucose levels interacted significantly with VLCBV on the prediction of PH (p-interaction = 0.011). In patients with VLCBV-regions, higher glucose was significantly associated with PH (adjusted-OR = 3.15; 95% CI = 1.08–9.19,p = 0.036), whereas this association was not significant in patients without VLCBV-regions. CBV values measured at pretreatment CTP in coregistered regions that developed PH or infarct at follow-up were not correlated with pretreatment glucose levels, thus suggesting the existence of alternative deleterious mechanisms other than direct glucose-driven hemodynamic impairments. Overall, these results suggest that both severe hypoperfusion and glucose levels should be considered in the evaluation of adjunctive neuroprotective strategies.
Reproducibility of small Ge2C6H10O7-added MgB2 bulks fabricated by ex situ Spark Plasma Sintering used in compound bulk magnets with a trapped magnetic field above 5 T
Authors: Badica P. , Aldica G. , Grigoroscuta M. A. , Burdusel M. , Pasuk I. , Batalu D. , Berger K. , Koblischka-Veneva A. , Koblischka M. R. 
Published: June 2020

AbstractBulk discs (20 mm diameter and 4.3 mm thickness) of MgB2 added with Ge2C6H10O7 were obtained by Spark Plasma Sintering. Six samples with composition MgB2(Ge2C6H10O7)0.0014 and one undoped sample were fabricated under similar conditions and were magnetically characterized in order to determine the scattering of properties and reproducibility. The main source of the scattering of the properties is the decomposition of the additive due to elimination of the organic part in gas form, which occurs stepwise with intensive vacuum drops at around ~ 560 and ~ 740 °C. A third drop, which is sometimes not well resolved being part of the second peak at 740 °C, occurs at ~ 820 °C. The critical temperature at the midpoint of the transition, Tc, shows only a relatively small variation between 37.4 and 38 K, and the irreversibility field at a low temperature of 5 K takes values between 8 and 10 T. The pinning force and pinning force related parameters do not correlate with the carbon substituting for boron in MgB2 and suggest a synergetic influence of the microstructural details and carbon. Overall, despite the superconducting properties scattering, the samples are of high quality. Stacked into a column of six samples, they can trap at the center and on the surface of the column a magnetic field of 6.78 and 5.19 T at 12 K, 5.20 and 3.98 T at 20 K and 2.39, and 1.96 T at 30 K. These promising values, combined with facile fabrication of the samples with relatively high quality and reproducibility, show the feasibility of their use in building complex and large compound arrangements for bulk magnets and other applications.
Wisteria floribunda agglutinin-positive Mac-2-binding protein as a diagnostic biomarker in liver cirrhosis: an updated meta-analysis
Authors: Feng Shu , Wang Zhonghao , Zhao Yanhua , Tao Chuanmin 
Published: June 2020

AbstractWisteria floribunda agglutinin-positive Mac-2-binding protein (WFA+-M2BP) had been suggested as a possible glycobiomarker for assessing liver fibrosis. Here, we conducted this updated meta-analysis to systematically investigate the predictive accuracy of WFA+-M2BP for diagnosing liver fibrosis and hepatocellular carcinoma (HCC) by comparing with multiple non-invasive indicators. We searched relevant literatures from Pubmed, Web of Science, EMBASE and Cochrane Library and enrolled 36 eligible studies involving 7,362 patients. Summary results were calculated using bivariate random effects model. The pooled sensitivities, specificities and areas under the summary receiver operating characteristic curves (AUSROCs) of WFA+-M2BP for identifying mild fibrosis, significant fibrosis, advanced fibrosis, cirrhosis, and HCC were 0.70/0.68/0.75, 0.71/0.75/0.79, 0.75/0.76/0.82, 0.77/0.86/0.88, and 0.77/0.80/0.85, respectively. The accuracy of WFA+-M2BP was strongly affected by etiology and it was not better than other non-invasive indicators for predicting early fibrosis. It showed similar diagnostic performance to hyaluronic acid and FibroScan for cirrhosis, but was equivalent to α-fetoprotein for HCC. In conclusion, WFA+-M2BP was suitable to diagnose late stage of liver fibrosis, especially cirrhosis. Individual cutoff value of WFA+-M2BP could be used to grade liver fibrosis in different etiology. Combined diagnostic model was suggested to improve its predictive accuracy for HCC.
Climate warming enhancement of catastrophic southern California debris flows
Authors: Ren Diandong , Leslie Lance M. 
Published: June 2020

AbstractThe sequence of wildfires followed by debris flows, frequently affects southern California, reflecting its drought-heavy precipitation climate bipolarity. Organic debris from incomplete burning is lighter than inorganic matter, and partially inviscid. Hence lower rainfall totals can trigger downslope motion than typically required by the underlying clasts of loose inorganic granular material. After advection downslope, the pebble-laden organic debris has a higher capacity for rilling; a positive feedback process. A mechanism is proposed whereby boulders are ‘rafted’ by organic debris. This coordinated movement of boulders greatly enhances the debris flow erosion capacity. This climate change sensitive debris flow enhancing mechanism, through organic–inorganic granular material interaction, is supported by observations and the numerical simulations. Using a model explicitly parameterizing erosion processes, including runoff entrainment, rilling incision, and bank collapse, the lifecycle of the Montecito debris flow of January 9, 2018 is simulated, providing quantitative estimates of mass conveyed and debris sorting at the terminus. Peak rafting speeds are ~ 12.9 m/s at ~ 300 m asl. Total boulder (effective diameter > 25 cm) volume involved for the Ysidro Creek area alone is ~ 5 × 104 m3, scattered between the region 50–260 m asl. Debris flows are highly repeatable and locations prone to debris flows are identified and their likelihood of realization estimated.
Associations of ficolins and mannose-binding lectin with acute myeloid leukaemia in adults
Authors: Sokołowska Anna , Świerzko Anna S. , Gajek Gabriela , Gołos Aleksandra , Michalski Mateusz , Nowicki Mateusz , Szala-Poździej Agnieszka , Wolska-Washer Anna , Brzezińska Olga , Wierzbowska Agnieszka , Jamroziak Krzysztof , Kowalski Marek L. , Thiel Steffen , Matsushita Misao , Jensenius Jens C. , Cedzyński Maciej 
Published: June 2020

AbstractWe investigated clinical associations of ficolins and mannose-binding lectin (MBL) in 157 patients suffering from acute myeloid leukaemia (AML). Concentrations of ficolin-1, ficolin-2, ficolin-3 and MBL (before chemotherapy) in serum were determined as were selected polymorphisms of the corresponding genes (FCN1, FCN2, FCN3andMBL2). The control group (C) consisted of 267 healthy unrelated individuals. Median level of ficolin-1 in patients was lower (p < 0.000001) while median levels of ficolin-2, ficolin-3 and MBL were higher (p < 0.000001,p < 0.000001 andp = 0.0016, respectively) compared with controls. These findings were generally associated with AML itself, however the highest MBL levels predicted higher risk of severe hospital infections (accompanied with bacteremia and/or fungaemia) (p = 0.012) while the lowest ficolin-1 concentrations tended to be associated with prolonged (> 7 days) fever (p = 0.026). Genotyping indicated an association ofG/Ghomozygosity (corresponding toFCN1gene − 542G > Apolymorphism) with malignancy [p = 0.004, OR = 2.95, 95% CI (1.41–6.16)]. Based on ROC analysis, ficolin-1, -2 and -3 may be considered candidate supplementary biomarkers of AML. Their high potential to differentiate between patients from non-malignant controls but also from persons suffering from other haematological cancers (multiple myeloma and lymphoma) was demonstrated.
HLA genotypes and cold medicine-induced Stevens–Johnson syndrome/toxic epidermal necrolysis with severe ocular complications: a systematic review and meta-analysis
Authors: Tangamornsuksan Wimonchat , Chanprasert Sirikan , Nadee Phenphechaya , Rungruang Saowalak , Meesilsat Natnaree , Ueta Mayumi , Lohitnavy Manupat 
Published: June 2020

AbstractSerious cutaneous adverse drug reactions [i.e., SJS/TEN with severe ocular complications (SOC)] associated with cold medicine (CM) were reported in several studies. To assess the risks of CM-induced SJS/TEN with SOC, systematic review and meta-analysis were employed. Studies investigating associations between HLA genotypes and CM-induced SJS/TEN with SOC were systematically searched in PubMed, Scopus and the Cochrane Library. Overall odds ratios (ORs) with 95% CIs were calculated using a random-effects model to determine these associations. An initial search of the databases identified 24,011 articles, of which 6 studies met the inclusion criteria. In total from all studies, associations between 81 different HLA genotypes and CM-induced SJS/TEN with SOC (i.e., 22 different HLA-A genotypes, 40 different HLA-B genotypes and 19 different HLA-C genotypes) were investigated. Risk factors to develop SJS/TEN with SOC in patients who used CM were identified from our meta-analysis. HLA-A*0206 (OR = 3.90; 95% CI = 1.96–7.77), HLA-A*3303 (OR = 2.28; 95% CI = 1.31–3.97), HLA-B*4403 (OR = 3.27; 95% CI = 1.52–7.03) and HLA-C*0501 (OR = 2.55; 95% CI = 1.19–5.44) were associated with CM-induced SJS/TEN with SOC. With our results demonstrating a significant association between using of CMs and the severe ADR, a genetic testing can be helpful. However, the CMs are commonly used as an over-the-counter drug in practically almost of people in populations worldwide, the genetic screening prior to use of the CMs might not be cost-effective. Nonetheless, for people with a family history of developing the ADRs with a possible involvement of CMs, a genetic screening may be beneficial.
Demographic census confirms a stable population of the critically-endangered caryophyllid coral Polycyathus chaishanensis (Scleractinia; Caryophyllidae) in the Datan Algal Reef, Taiwan
Authors: Kuo Chao-Yang , Keshavmurthy Shashank , Chung Aichi , Huang Ya-Yi , Yang Sung-Yin , Chen Yei-Chia , Chen Chaolun Allen 
Published: June 2020

AbstractPolycyathus chaishanensisis a symbiotic caryophyllid coral described from a single population in a tidal pool off Chaishan, Kaohsiung, Taiwan. Due to its rarity,P. chaishanensiswas declared a critically-endangered species under the Taiwan Wildlife Protection Act. In May 2017, aP. chaishanensiscolony was discovered in the intertidal area of the Datan Algal Reef, Taoyuan, Taiwan. To determine whether this is a stable population in the algal reef, a demographic census—including data on occurrence, distribution, and colony size—was carried out in the algal reef in southern Taoyuan. Intertidal censuses and sediment collections were conducted at five different sections—Baiyu, Datan G1, Datan G2, Yongxing, and Yongan algal reefs—during the monthly spring low tide from July 2018 to January 2019. In total, 84 colonies—23 in Datan G1 and 61 in Datan G2—were recorded from a tidal range of − 160 to − 250 cm, according to the Taiwan Vertical Datum 2001 compiled by the Central Weather Bureau. NoP. chaishanensiswas found in Baiyu, Yongxing, or Yongan. TheP. chaishanensiscolony sizes ranged from 2.55 to 81.5 cm in diameter, with the largerP. chaishanensispresent in the lower intertidal zone. Sediment was extremely high, with monthly site averages ranging from 3,818.26 to 29,166.88 mg cm−2day−1, and there was a significant difference between sites and months, both of which affected the distribution ofP. chaishanensisin the algal reef. Our study confirms the existence of a second population ofP. chaishanensisin Taiwan, highlighting the importance of the Datan Algal Reef for the survival and protection of this critically-endangered caryophyllid coral and why it is so urgent that the reef should be conserved.
Design and Development of a Speech Intelligibility Test Based on Pseudowords in French: Why and How?
Authors: Lalain Muriel , Ghio Alain , Giusti Laurence , Robert Danièle , Fredouille Corinne , Woisard Virginie 
Published: June 2020

PurposeThe current intelligibility tests performed on speakers with atypical speech production are limited by the ability of listeners to restore distorted sequences. This results in a measure that is overvalued when compared with the real articulatory performance. In this article, we present a new intelligibility test in order to neutralize the commonly encountered bias in traditional perception-based assessments. We present the construction of the acoustic–phonetic decoding task and its first test during a perceptual judgment test of intelligibility and during a result comparison with a global perceptual evaluation.MethodWe developed a very large pseudoword directory including about 90,000 forms that respect French phonotactic constraints. From this directory, we have created lists of pseudowords intended to be recorded for the constitution of the corpus. These lists are established due to an algorithm integrating predefined linguistic constraints and produced by 47 speakers (nine healthy and 38 patients). We then performed a perceptual judgment of intelligibility test with 20 listeners who transcribed these productions.ResultsAt the end of the data processing stage, we obtained a Perceived Phonological Deviation (PPD) score for each speaker that reflects the average number of features altered per phoneme. We then compared the PPD score with a global intelligibility score derived from a global perceptual assessment of intelligibility and of the alteration severity.ConclusionsThe current findings confirm that a speech intelligibility test based on pseudowords in French achieves fine-grained PPD scores, which enables discrimination between patients and healthy speakers. Moreover, the PPD score is related to the global intelligibility score, especially in severity. Further studies are needed to better understand the discrimination power of this intelligibility test based on an acoustic–phonetic decoding task.