bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicting Risk of Chronic Hypertension in Women with Preeclampsia Based on Placenta Histology. A Prospective Cohort Study in Cuba.
Authors: Byonanuwe Simon , Fajardo Yarine , Nápoles Danilo , Alvarez Alexis , Cèspedes Yaimi , Ssebuufu Robinson 
Published: August 2020

Abstract
Background: Chronic hypertension is one of the major sequelae of preeclampsia with associated long term morbidity and mortality among previously normotensive women diagnosed with preeclampsia. We identified the predictors of developing this complication in women with preeclampsia admitted at the Carlos Manuel de Cèspedes Teaching Hospital in Cuba based on histological assessment of their placenta so as to guide the health care providers in early identification of the patients at risk for timely intervention against this vicious condition. Methods: A three-year prospective cohort study was conducted between March 2017 and March 2020. A cohort of 178 women diagnosed with preeclampsia was recruited and followed up at 12 weeks postpartum for chronic hypertension. Histological studies were done on their placenta following delivery and the respective result forms used to collect the data. Cox’s hazards regression model was applied to estimate the risk using STATA version 14.2.Results: Chorioamnionitis (HR=1.697, 95%CI: 1.443-3.416, p=0.038), villositary infarcts (HR=1.657, 95%CI: 1.264-2.848, p=0.048), intervillositary thrombus (HR=1.529, 95%CI: 1.231-3.197, p=0.020), and endarteritis (HR=1.242, 95%CI: 1.115-1.804, p=0.025) placental lesions were predictive of chronic hypertension at 12 weeks postpartum.Conclusion: Placental histology in women with preeclampsia is key towards improving the ability to diagnose and monitor those likely to develop chronic hypertension before its onset for timely intervention.
Adalimumab and Anti-adalimumab Lisa-tracker Immunoassays Performance Criteria for Therapeutic Drug Monitoring of Adalimumab-amgen Biosimilar (Abp501)
Authors: FRANCOIS Fabien , NAIMI Loubna , ROBLIN Xavier , Berger Anne-Emmanuelle , Paul Stephane 
Published: August 2020

Abstract
Background.
ABP501 is a biosimilar to Reference Adalimumab (HUMIRA®) produced by AMGEN. Adalimumab (ADA) has marketing authorization for Crohn's disease, ulcerative colitis and other inflammatory or autoimmune diseases. The aim of this study was to evaluate the LISA-TRACKER assays developed by Theradiag (France), for the monitoring of ABP501 and anti-ABP501 antibodies in human serum.
Methods.
Accuracy of the LISA-TRACKER was measured using 3 human serum matrices spiked with known levels of biosimilar, 3 levels spanning the dynamic range. Specificity was tested with Biosimilar spiked samples, Biosimilar with Humira® spiked samples, and clinical samples from patients treated with adalimumab biosimilar. All of these samples were spiked with polyclonal antibodies directed against Humira®. Intra-run, inter-run imprecision, inhibition, kit’s stability, specificity inhibition and specificity detection steps were also part of the LISA-TRACKER Duo Adalimumab assay validation parameters.
Results.
68 ABP501 clinical samples were measured with the LISA TRACKER Duo Adalimumab assay. LISA TRACKER has been validated as suitable for quantification of ABP501 in human serum samples. Concerning accuracy, percentages of recovery were ranged from 90–120% for biosimilar batch1, and between 93% and 105% for biosimilar batch2. The acceptance criteria (CV < 20%) were met for intra-run (from 3.8–16.5%) and inter-run imprecision (from 4.4–13.9%) including the two batches. All results were comprised within +/-20% from results, obtained with the kit and sample unexposed in order to evaluate stability of the sample, stability of the kit and consistency of the results. In any case but two, all percentages of inhibition were > 50% for specificity. Reagents made with ABP501 gave similar results than reagents made with Humira® meeting acceptance criteria.
Conclusions.
LISA-TRACKER ADA and anti-ADA assays are reliable for the monitoring of patients treated with ABP501.
Face Masks Use in the Public Domain and its Determinants During the SARS-Cov-2 Epidemic in Poland; a Non-Participatory Covert Observational Study
Authors: Ganczak Maria , Pasek Oskar , Duda-Duma Łukasz , Świstara Dawid , Korzeń Marcin 
Published: August 2020

Abstract
Background: Face masks play an important role in protection from acquiring SARS-Cov-2, however, if used incorrectly they may increase transmission risk. Many countries, including Poland, currently recommend the use of masks in their pandemic control plans. The study objective was to assess the practices of the public in the case of face masks/N95 respirators utilization during the SARS-Cov-2 pandemic in Poland and to evaluate factors influencing their use.Methods: This non-participatory covert observational study was conducted on 3 separate occasions, (10.05/18.05/25.05.2020). At each point, 82 medical students observed 10 consecutive individuals (2460 total) appearing in the public space in 13 Polish regions which differed regarding SARS-Cov-2 pandemic risk. A structured observation checklist served as a survey tool.Results: A total of 2353 observations were included; the female/male ratio was similar (1.02-1.13-1.03 respectively) at the 3 occasions, as well as the age distribution, with those aged 21-60 years predominant (70.8%-71.9%-70.2% respectively). Most of participants (73.6%; 552/750) were using facial masks at the first time point, which decreased in the 2nd and 3rd week (544/818; 66.5% and 516/785; 65.7% respectively). A predominance of cloth masks was observed at all time points (64.7%-62.3%-62.6% respectively) followed by medical masks (23.4%-28.5%-26.9% respectively). Female gender (OR=1.75-1.47-1.53 respectively), age >40 years (OR=1.46-1.48 respectively), a closed space (OR=2.56-2.63-2.36 respectively) were each associated with higher mask usage. Those playing sports were about two times less likely to use facial masks (OR=0.64-0.54-0.54 respectively) than when compared to other activities. The percentage of those using masks correctly decreased gradually over time (364/552; 65.9%; 339/544; 62.3% and 304/516; 58.9% respectively); more females wore masks correctly (p<0.05). Breaches in nose covering (47.3%-52.7%) and hanging masks around the neck (39.2%-42.6%) were the most common incorrect practices while wearing a mask. Conclusions: Cloth masks were predominantly used in the public space. Practices regarding the facial masks usage were found to be inadequate, especially among young males, and tended to decrease over time. Awareness campaigns regarding the need of the proper use of face masks by utilizing all communication channels available would be helpful during this pandemic to increase compliance.
Women's appraisal, interpretation and help-seeking for possible symptoms of breast and cervical cancer in South Africa: a qualitative study
Authors: Harries Jane , Scott Suzanne E , Walter Fiona M , Mwaka Amos D , Moodley Jennifer 
Published: August 2020

Abstract
Background:
In South Africa, breast cancer is the most commonly diagnosed cancer and cervical cancer the leading cause of cancer mortality. Most cancers are diagnosed at a late-stage and following symptomatic presentation. This qualitative study set out to explore appraisal, attribution and management of potential breast and cervical cancer symptoms at a community level among undiagnosed women.
Methods:
In-depth interviews were conducted with women with potential breast or cervical cancer symptoms from urban and rural South Africa. Participants were recruited from a community-based cross-sectional study on breast and cervical cancer awareness. Data were analysed using a thematic analysis approach.
Results:
Eighteen women were interviewed (10 urban, 8 rural): the median age was 34.5 years (range 22–58). Most were unemployed, and five were HIV positive. Themes included impact and attribution of bodily changes; influence of social networks and health messaging in help-seeking; management of symptoms and help-seeking barriers. Breast changes were often attributed to manual activities or possible cancer. Women were often unsure how to interpret vaginal symptoms, attributing them to HIV, hormonal contraceptives, or partner infidelity. Concerns about cancer were based on health information from the radio, social networks, or from primary care providers. Prompt care seeking was triggered by impact of symptoms on personal lives. Rural women, especially with possible symptoms of cervical cancer, experienced challenges during help-seeking including judgmental attitudes of clinic staff. Most participants were skeptical of traditional medicine.
Conclusions:
This is the first study exploring interpretation of possible breast and cervical cancer symptoms at a community level in South Africa. The process of interpreting bodily changes, symptom attribution and help-seeking is complex and influenced by women’s everyday life experiences. Timely diagnosis interventions should not only include cancer symptom awareness but also address individual, structural and health systems related barriers to care.
Examining the sensitivity of the surveillance system to detect TB cases in Afghanistan
Authors: Hamdard Naqibullah , Atarud Alim , Seddiq Khalid , Hanif Anwar 
Published: August 2020

Abstract
BackgroundTuberculosis (TB) remains a global public health threat. World Health Organization (WHO) End TB strategy recommends that effective TB control relies on general health systems, especially, on integrated and well-functioning PHC facilities. Despite, integration in Basic Package of Health Services (BPHS), evidence demonstrates that a large number of TB cases are not captured. 25000 TB cases are missed every year (Aloudal, 2015). 49% of individuals, recorded at health facilities as presumptive to have TB have not been screened (HMIS, 2016). This study intended to evaluate different dimensions of TB surveillance system and the Primary Health Care (PHC) facilities' role in TB active case finding. MethodsWe conducted a cross-sectional study. The study was implemented in eleven provinces of Afghanistan in from August to November 2016. The geographic and demographic representativeness determined the choice of provinces. All primary health care facilities in studied provinces equated to 870, therefore, considering a 95 % confidence interval, a sample size of 161 facilities established the sample frame. A stratified sampling strategy facilitated the selection of sampled facilities within different categories from an inclusive list of all facilities.ResultsThe study found that the overall sensitivity of TB surveillance systems in-country is 56.30 %. This means that 43.70 % of 171 TB cases had remained undetected. 43.81 % of studies facilities used at least one accepted active case finding strategy while 56.20 % implemented none. In studied areas, 83.33 % of 11 studied DHs and 69.57 % of 35 studied CHCs had a referral system for MDR-TB patients.ConclusionTo enhance sensitivity and boost case findings, it is essential to implement case-finding strategies targeted at high-risk groups in specific areas. The high-risk groups include IDPs, returnees, slum residents, prisoners, and addicts. Additionally, it is necessary to train private pharmacists and traditional healers to identify and refer individuals with TB symptoms for follow up and further evaluation at the PHC level.
Comparison of Pregnancy Outcomes in Young Patients Following Fresh Versus Frozen Single Blastocyst Transfer: A Retrospective Study
Authors: Wu Yanhong , Liu Chang , Sheng Chaochao , Xi Haitao , Fu Yanghua , Zhao Junzhao 
Published: August 2020

Abstract
Background：Previous studies have shown that in young women, single blastocyst transfer can achieve satisfactory pregnancy results, but there are few studies on the difference between fresh and frozen-thawed single blastocyst transfer. In this retrospective analysis, the purpose of this study was to compare the pregnancy outcomes of young patients who received fresh or frozen-thawed single blastocyst transfer, so as to provide further clinical strategies for single blastocyst transfer.Methods: A retrospective analysis of patients aged ≤35 years old undergoing single blastocyst transfer was performed from January 2018 to December 2018 in the reproductive center of the Second Affiliated Hospital of Wenzhou Medical University. A total of 901 patients were involved and were divided into two groups according to the type of transfer cycle, 693 of which were in the fresh embryo transfer cycle (group A) and 208 of which were in the frozen-thawed embryo transfer cycle (group B). The laboratory and pregnancy outcomes were compared between the groups. Results：The number of oocytes retrieved in group B was significantly more than in group A ( P＜0.05). The early miscarriage rate in group B was higher than in group A, with significant difference(P＜0.05). There were no significant differences in other basic conditions and obstetric pregnancy outcomes between the two groups.Conclusions：Favorable pregnancy outcomes could be obtained in both fresh and frozen-thawed single blastocyst transfer in young patients without increasing the birth defect rate. For patients with risk factors of OHSS such as more oocyte retrieval, ovarian hyper-response, or risk factors of ectopic pregnancy such tubal infertility and the history of ectopic pregnancy, frozen-thawed single blastocyst transfer could be highly recommended in clinical practice. However, since the early miscarriage rate is higher in frozen-thawed embryo transfer group, further research is needed to figure out the cause and possible solutions.
Women’s reasons for declining cardiovascular screening – a qualitative study
Authors: Dahl Marie , Andersen Anne Bendix , Heggen Kristin Margrete , Soendergaard Susanne Friis 
Published: August 2020

Abstract
Background

During 2011–2013, women from a municipality in Denmark who were born in 1936, 1941, 1946 or 1951 were invited to cardiovascular screening (n = 1984); of those, ten nonattendees were interviewed about their perspectives on cardiovascular screening. The interviews were re-analysed to gain a deeper understanding of their motives for viewing screening as personally irrelevant. A salutogenic perspective formed the premise for the data analysis.
Methods

A secondary analysis applying a deductive content analysis inspired by Elo and Kyngäs. The core components of Sense of Coherence were used as a theoretical framework.
Results

We found that nonattendance was rooted in the women’s social role as caregiver and their individual inner logics. Being a caregiver provided the women with a feeling of Sense of Coherence in their daily lives. The inner logics reflected a line of reasoning without critical reflections and the women acted upon these when declining screening. Inner logics were used as a strategy to uphold their social role and identity.
Conclusion

The women had a salutogenic orientation to life and they found screening meaningless. Inner logics, caring role and personal desire to maintain control of one’s life interact with individuals’ experiences of Sense of Coherence, and thus their identities. If women are expected to attend screening, it must be emotionally and cognitively meaningful for them. This could be facilitated by using a salutogenic approach in the screening invitation.
Multi-trait GWAS using high-density genotypes identifies genes associated with body traits in Nile tilapia
Authors: Yoshida Grazyella , Yáñez José Manuel 
Published: August 2020

Abstract
Background: Body traits are generally controlled by several genes in vertebrates (i.e. polygenes), which in turn make them difficult to identify through association mapping. Increasing the power of association studies by combining approaches such as genotype imputation and multi-trait analysis improves the ability to detect quantitative trait loci associated with polygenic traits, such as body traits. Results: A multi-trait genome-wide association study (mtGWAS) was performed to identify quantitative trait loci (QTL) and genes associated with body traits in Nile tilapia (Oreochromos niloticus) using genotypes imputed to whole-genome sequence (WGS). To increase the statistical power of mtGWAS for the detection of genetic associations, summary statistics from single-trait genome-wide association studies (stGWAS) for eight different body traits recorded in 1,309 animals were used. The mtGWAS increased the statistical power from the original sample size from 13% to 44%, depending on the trait analyzed. The better resolution of the WGS data combined with the increased power of the mtGWAS approach, allowed the detection of significant markers not previously found in the stGWAS. Some lead single nucleotide polymorphisms (SNPs) were found within important functional candidate genes previously associated with growth-related traits. For instance, we identified SNP within the α1,6-fucosyltransferase (FUT8), solute carrier family 4 member 2 (SLC4A2), A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) and heart development protein with EGF like domains 1 (HEG1) genes, which have been associated with average daily gain in sheep, osteopetrosis in cattle, chest size in goats, and growth and meat quality in sheep, respectively. Conclusions: The high-resolution mtGWAS presented, allowed identification of significant SNPs, linked to strong functional candidate genes, associated with body traits in Nile tilapia. These results provide further insights about the genetic variants and genes underlying body trait variation in cichlid fish with high accuracy and strong statistical support.
The embryonic stem cell microenvironment inhibits mouse glioma cell proliferation by regulating PI3K/AKT pathway
Authors: He xiongjun , liu jiahui , wang xiaoran , liu tengfei , yang liu , li chaoyang , wang chenjie , liu ying , sang xuan , wang zhichong , lu xiaohe 
Published: August 2020

Abstract
PURPOSETo study the effect of the embryonic stem cell (ESc) microenvironment in inhibiting mouse glioma cells and its possible mechanism. METHODSGlioblastoma cell line U118 in the brain, was investigated in this study. There were four experimental groups: U118 glioma cells cultured alone as the control group (GB group), U118 cell and ESc co-cultured group(GE group), U118 cell and ESc co-cultured and adding phosphoinositide 3-kinase (PI3K) agonist group (GA group), U118 and temozolomide as the positive control group (GT group); U118 cells were harvested after 72 hours of culture. Cell proliferation, apoptosis, reactive oxygen species (ROS) and vasculogenic mimicry assays, quantitative real-time RT-PCR (qPCR), and western blot (WB) were study, the biological function of U118 glioma cells and the PI3K/AKT signaling pathway were compared the differences between the groups. RESULTSCompared with the GB control group, the GE co-culture group and GT chemotherapy group showed reduced cell proliferation, increased apoptosis, increased ROS, decreased or disappearance of vasculogenic mimicry. Expression of cyclin B and D, significantly reduced, while that of BAX, BCL-2, P53, Caspase3, Gsk-3B, P21, and P27, significantly increased. The expression of PI3K, PDK, AKT, and mTOR, significantly decreased while that of PTEN significantly increased. The expression of positive regulatory factors significantly increased but negative regulatory factors decreased in GA group compared to the GE group. CONCLUSIONThe ESC microenvironment reverse glioma malignancy, partially via inhibition of the PI3K signaling pathway. Our study may have a significant impact and clinical implication on cell therapy in glioma.
Supervised machine learning models and protein-protein interaction network analysis of gene expression profiles induced by omega-3&nbsp;polyunsaturated fatty acids
Authors: Shityakov Sergey , Chang Jane Pei-Chen , Sun Ching-Fang , Guu David Ta-Wei , Dandekar Thomas , Su Kuan-Pin 
Published: August 2020

Abstract
BackgroundOmega-3 polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids have beneficial effects on human health but their effect on gene expression in elderly individuals (age ≥ 65) is largely unknown. To examine this, the gene expression profiles were analyzed in the healthy subjects (n = 96) at baseline and after 26 weeks of supplementation with EPA+DHA to determine up-regulated and down-regulated differentially expressed genes (DEGs) triggered by PUFAs. The protein-protein interaction networks were constructed by mapping these DEGs to a human interactome and linking them to the specific pathways.ResultsThe results revealed that up-regulated DEGs were associated with neurotrophin/MAPK signaling, whereas the down-regulated DEGs were linked to the cancer, acute myeloid leukemia, and long-term depression pathways. Additionally, machine learning (ML) approaches were able to cluster the EPA/DHA-treated and control groups by the logistic regression algorithm performing the best. ConclusionOverall, this study highlights the pivotal changes in DEGs induced by PUFAs and provides the rationale for the implementation of ML algorithms as predictive models for this type of biomedical data.
Revealing the intrinsic superconducting gap anisotropy in surface-neutralized BaFe2(As0.7P0.3)2
Authors: Xin Ziming , Wang Yudi , Cai Cong , Wang Zhengguo , Chen Lei , Han Tingting , Zhang Yan 
Published: August 2020

Abstract
Alkaline-earth iron arsenide (122) is one of the most studied families of iron-based superconductors, especially for angle-resolved photoemission spectroscopy. Extensive results have been obtained including band structure, gap anisotropy, etc. However, the complicacy of 122 caused by its charge-non-neutral cleavage surface is rarely considered. Here, we show that the surface of 122 can be neutralized by potassium deposition. In potassium-coated BaFe2(As0.7P0.3)2, the surface-induced spectral broadening is strongly suppressed, while the coherent spectra that reflects the intrinsic bulk electronic state recovers. This raises the accuracy of the gap measurement and gap fitting to an unpreceded level. The results clearly distinguish two pairing channels originated respectively from the inner and outer Fermi pockets. While the gap anisotropy on the outer hole/electron pockets can be well fitted using an s± gap function, the gap magnitude on the inner hole/electron pockets show a clear deviation. Our results provide quantitative constraints for refining theoretical models and demonstrate an experimental method for revealing the intrinsic electronic properties of 122 in future studies.