bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antioxidant capacity of lipid- and water-soluble antioxidants in dogs with subclinical myxomatous mitral valve degeneration anaesthetised with propofol or sevoflurane
Authors: Tomsič Katerina , Svete Alenka Nemec , Nemec Ana , Petrič Aleksandra Domanjko , Pirman Tatjana , Rezar Vida , Vovk Tomaž , Seliškar Alenka 
Published: March 2020

Abstract
Background

Antioxidants located in both the hydrophilic and lipophilic compartments of plasma act as a defence system against reactive oxygen species (ROS), which are continuously generated in the body due to both normal metabolism and disease. Excessive production of ROS during anaesthesia affects the antioxidant capacity of plasma and may result in oxidative stress. The aim of this study was to evaluate the antioxidant capacity of lipid- (ACL) and water-soluble (ACW) antioxidants in client-owned dogs diagnosed with periodontal disease and myxomatous mitral valve degeneration (MMVD) and anaesthetised for a dental procedure with propofol and sevoflurane or with propofol only.

Results

Dogs with MMVD were anaesthetised with propofol and sevoflurane (MMVD/PS, n = 8) or with propofol only (MMVD/P, n = 10). Dogs with no evidence of MMVD (PS, n = 12) were anaesthetised with propofol and sevoflurane. Blood samples for determination of ACL and ACW were collected before and 5 minutes, 60 minutes and 6 hours after induction to anaesthesia. In dogs with MMVD and anaesthetised with propofol and sevoflurane, ACL was significantly higher at all sampling times when compared to control dogs. In dogs with MMVD and anaesthetised with propofol, ACW increased after induction to anaesthesia and remained elevated up to 6 hours after anaesthesia. The only difference between propofol and propofol/sevoflurane anaesthesia in dogs with MMVD was significantly higher ACW at 60 minutes after induction to anaesthesia in the propofol group.

Conclusions

Compared to basal values, anaesthesia maintained with either propofol or sevoflurane significantly increased ACW in both dogs with and without MMVD. In dogs with MMVD, ACW was significantly higher in the propofol group. Furthermore, only anaesthesia with propofol significantly increased ACL in dogs with MMVD. These results suggest that regarding antioxidant capacity, propofol might be a better choice than sevoflurane for anaesthesia in dogs with MMVD.
5-Year (2014-2018) Trends of Visceral Leshmaniasis in Amhara Region, North west Ethiopia
Authors: Zeru Taye 
Published: March 2020

Abstract
Background: Visceral leishmaniasis (VL; also known as kala-azar), caused by protozoan parasites of the Leishmania donovani complex, is a major public health problem in Ethiopia. Despite VL is endemic in the lowlands of Amhara region, and is almost always fatal if left untreated, there is no report that shows regional wide trends of VL cases over years of period.Method: A retrospective data to determine cumulative number of cases, trends in incidence and mortality rate, and geographic coverage of VL over 5 years period (2014-2018) was obtained from Health Management Information System (HMIS) database owned by Amhara Regional Health Bureau Leishmaniasis Service. SPSS version 20 was used to analyze the data.Results: Over 5 years of period (2014-2108), a total of 5,624 VL cases were registered across all zones of Amhara Region.The majority of these VL patients (90%) were adult while, men being the most affected ones (87%) than women (13%). High incidence rate of VL with a trend of steady increment over the 5 years period was observed. The highest fatality rates were recorded in North Gondar zone and No fatality rates were recorded in North Wollo , South wollo ,North Shoa ,East gojam and West gojam respectively. With regard to health service attendance, 58% of VL cases were register outpatient attendees.Conclusion: VL was reported in almost all parts of the Amhara region posing a serious challenge on provision of diagnosis and treatment services, especially in areas where the disease has never been reported before. Additionally, adult men representing the working group of the community were the most sufferers of VL, suggesting the bad consequence on the socio-economy of the region. To overcome those challenges associated with VL, training health care providers on prompt diagnosis and clinical management of VL along with other strategies is imperative.
Association between risk of preeclampsia and maternal plasma trimethylamine-N-oxide in second trimester and at the time of delivery
Authors: Huang Xin , Li Zuodong , Gao Zhou , Wang Dapeng , Li Xiaohui , Li Ying , Mi Chunmei , Lei Jun 
Published: March 2020

Abstract
Background: The data on the association between the microbiota-dependent metabolite trimethylamine-N-oxide (TMAO) during pregnancy and risk of preeclampsia (PE) is limited. Methods: We, therefore, conducted a prospective nested case control study during Sep 2017 to Dec 2018 to examine the association between plasma TMAO measured during pregnancy and the risk of PE. Total of 17 patients diagnosed with EOPE (early onset PE), 49 with LOPE (late onset PE) and 198 healthy controls were enrolled. Blood samples were collected at 15-23 gestational weeks and time at delivery. The Logistic regression model was used to assess the odds ratio (OR) and 95% confidence interval (CI) for TMAO and risk of PE, EOPE, LOPE, mild PE, and severe PE. Results: We found that the mean TMAO levels of overall subjects in the second trimester (T2) and at the time of delivery (TD) were 90.39 µg/m 3 (SD=45.91) and 175.01 µg/m 3 (SD=160.97), respectively. No significant spearman correlation was found between the TMAO in those two periods ( p > 0.05). T2 TMAO was not significantly associated with risk of PE or risk of any PE subtypes ( p >0.05). However, TD TMAO was significant associated with risk of PE, EOPE and severe PE (adjusted OR and 95%CI were 1.24(1.09, 1.40), 1.62(1.29, 2.03), and 1.41(1.17, 1.70)) per 50µg/m 3 increment, respectively). Conclusion: Our study found that plasma TMAO level would alter over the course of pregnancy. The major role of TMAO in PE development might be in the accelerating process not in the initiation.
The accuracy and influencing factors for preference of self-sampling in group B streptococcus screening: a cross-sectional study
Authors: Chen Ruirui , Wu Lijuan , Ma Fenglan , Chen Xuri , Zhu Yuanfang 
Published: March 2020

Abstract
Background Self-sampling with proper instruction in 35-37 weeks’ gestation is supplementary to clinician sampling to prevent early-onset invasive group B streptococcal disease of infants. Despite of the accuracy proved in previous studies, disputes were raised on pregnant women’s low preference and adherence to the method of swab collection. We aimed to assess the accuracy of self-sampling and influencing factors for preference on collection method in Chinese women. Methods We compared screening results of self-sampling with clinician collection in a sample of 522 women in late pregnancy. These participants needed to complete a questionnaire on their preference and demographics after self-sampling. A multi-nominal logistic regression model was then used to measure the association between the influencing factors and preference for collection method. Results A good agreement between the two collection methods was found with a Cohen’s Kappa coefficient 0.83 (95%CI 0.71-0.95). The prevalence of GBS infection in the two methods is statistically different. Four factors (maternal age, parity, education attainment and pain difference) were included in the final multi-nominal regression model while gestational age and vaginal suppository use were excluded. Non-elderly parturient women were 2.84 (95% CI 1.19-6.74) times more likely to prefer self-sampling compared to clinician sampling, adjusting for parity, education and pain difference. If these participants experienced more or equal pain during self-sampling compared to clinician collection, they were more likely to prefer clinician sampling controlling the other three factors’ effect. Conclusions Our study suggests high agreement between the two collection methods. Self-sampling presented a higher detection rate than physician-collected samples. Pregnant women are able to collect rectovaginal samples prior to their antenatal visit. Self-sampling is preferable by 1/5 of the participants and it could be an option for those younger than 35 years old, especially for those with low pain threshold.
Determination of the contributions of degenerate and non-degenerate two-photon absorption response in silicon avalanche photodiode for infrared photon detection
Authors: Xu Guangjian , Ren Xinyi , Miao Qucheng , Yan Ming , Pan Haifeng , Chen Xiuliang , Wu Guang , Wu E 
Published: March 2020

Abstract
The study of non-linear interactions in semiconductor photo-electronic devices to achieve effective and fast photon identification is an ongoing and important task. We investigated the specific contribution of degenerate and non-degenerate two-photon absorption (D-TPA and ND-TPA) response in silicon avalanche photodiode (Si-APD) for infrared photon detection at room temperature. We experimentally demonstrated that when the two laser pulses overlapped, the average D-TPA quantum detection efficiencies at 1800 nm, and that at 1550 nm were measured as 3.3×10 -16 counts•pulse/photon 2 , 4.3×10 -15 counts•pulse/photon 2 , respectively. And the ND-TPA quantum detection efficiency of 1800 nm and 1550 nm was measured to be 7.9×10 -16 counts•pulse/photon 2 . This study provides a solution for the practical infrared photon detection devices based on TPA effect in Si-APDs.
Role of CASP7 polymorphisms in noise-induced hearing loss risk in Han Chinese population
Authors: Ruan Yanmei , Zhang Jinwei , Mai Shiqi , Zeng Wenfeng , Huang Lili , Gu Chunrong , Liu Keping , Ma Yuying , Wang Zhi 
Published: March 2020

Abstract
Background: Genetic factors and gene-environment interaction may play an important role in the development of noise induced hearing loss (NIHL). This study explored the relationship between the SNP of CASP genes and the NIHL susceptibility of Chinese workers exposed to occupational noise.Methods: 191 cases and 191 controls were selected by 1:1 case control study. Among them, case groups were screened from workers exposed to noise at binaural high-frequency hearing thresholds greater than 25 dB (A). The control group selected workers with hearing thresholds≤25 dB (A) in any binaural frequency band based on matching factors such as age, exposure time to noise, and operating position. The blood samples of two groups of workers were subjected to DNA extraction and SNP sequencing of CASP3 and CASP7 genes using the polymerase chain reaction - ligase detection reaction method. Conditional logistic regression correction was used to analyze genetic variation associated with susceptibility to NIHL. Odds Ratios (OR) and 95% confidence intervals (CI) were calculated.Results: There was an association between rs2227310 and rs4353229 of the CASP7 gene and the risk of NIHL. Compared with the GG genotype, the CC genotype of rs2227310 reduced the risk of NIHL (OR = 0.453, 95% CI = 0.241 ~ 0.852). Compared with CC genotype, the TT genotype of rs4353229 reduced the risk of NIHL (OR = 0.458, 95% CI = 0.243 ~ 0.862). Workers carrying the rs2227310GG and rs4353229CC genotype had an increased risk of NIHL compared to workers without any high-risk genotype (OR = 1.839, 95% CI = 1.033 ~ 3.274). The CASP7 gene interacted with factors such as age, smoking status, dietary taste, and conscious auditory system symptoms on the risk of NIHL (P <0.05).Conclusions: The single nucleotide polymorphism of CASP7 gene interacts with factors such as age, frequency of smoking, dietary taste, and conscious auditory system symptoms on the risk of NIHL.
Trends and seasonal variation of hospitalization and mortality of interstitial lung disease in the United States from 2006 to 2016
Authors: Ho An , Shmelev Artem , Charbek Edward 
Published: March 2020

Abstract
BackgroundIn the recent years, the overall trends in hospital admission and mortality of interstitial lung disease (ILD) are unknown. In addition, there was some evidence that interstitial lung disease death rate highest in the winter but this finding was only available in one study. This study will investigate the trend and seasonal variations in hospital admission and mortality rates of ILD from 2006 to 2016.MethodFrom the Nationwide Inpatient Sample database, we collected all cases with the International Classification of Diseases (ICD)-9 or ICD-10 codes of ILD excluding identifiable external causes (drug, organic or inorganic dusts) from 2006 to 2016. Hospitalization rates of each year were calculated based on U.S Census population data. Monthly hospitalization and in-hospital mortality rates were analyzed by seasonal and trend decomposition.ResultsFrom 2006 to 2016, there was a downtrend in all cause hospital admissions but in-hospital mortality rate did not change, with or without the presence of pneumonia. Highest hospital admission rates of ILD per 100000 of population were from January to May. The average number of hospitalizations in spring, summer, fall and winter months were 7447.9 ± 932.0, 6643.0 ± 840.5, 6551.3 ± 922.6 and 7110.3 ± 866.1 respectively. All-cause in-hospital mortality ranges from 7.13% ± 0.79% in the summer to 8.13% ± 0.60% in the winter with winter months having the highest mortality rate (p=0.018). The seasonal variations of hospital admission and mortality was not changed when infectious pneumonia cases were ruled out.ConclusionFrom 2006 to 2016, admission rates of ILD declined but in-hospital mortality remained unchanged. All-cause hospital admissions and mortality of ILD have a strong seasonal variation. Hospital admissions are highest in the period from January to May, in-hospital death was highest in the winter.
Three-fourth of children is received early initiation of Breastfeeding in West Belessa District
Authors: Ayalew Desalew Degu , Kassie Belayneh Ayanaw , Hunegnaw Melkamu Tamir , Alemu Kassahun , Belew Aysheshim Kassahun 
Published: March 2020

Abstract
Background : The world is now suffering from malnutrition and remains one of the major causes of under-five mortality. Children from developing countries, including Ethiopia also suffer from undernutrition due to suboptimal breastfeeding practice. Hence, the study aims were to assess early initiation of breastfeeding among children aged less than two years, months in West Belessa district, North West Ethiopia, 2019. Methods: A community-based cross-sectional study was conducted from January to February 2019 in the West Belessa district. A total of 569 Children was selected by a simple random sampling technique. The data were collected by an interviewer-administered structured questionnaire. Data were entered and analyzed by using Epi-Info version 7 and SPPS version 20, respectively. Bi-variable logistic regression analysis was used to check predictor variables associated with the dependent variable. Variables having a p-value of less than 0.20 in the bi-variable analysis were fitted into the multivariable model. Multivariable binary logistic regression with a 95% confidence interval and Odds Ratio (OR) were computed. Variables having p-value < 0.05 were taken as significantly associated with the dependent variables. Result : The prevalence of EIBF was 77.7 % (95%CI, 74.3-81.0). Age of the mother (AOR= 2.76, 95%CI (1.21, 6.27)), postnatal Visit (AOR= 1.85, 95%CI (1.03, 3.85)), and Antenatal care (AOR= 2.58, 95%CI (1.18, 9.94)) was significantly associated with EIBF. Conclusion and Recommendation: We observed, the prevalence of early initiation of Breastfeeding was low in West Belessa District. Age of the mother, Antenatal and postnatal care were associated with EIBF. Hence, improving antenatal and postnatal care services through increase accessibility and providing counseling during this contact time. Keywords: early initiation of breastfeeding, Children, West Belessa
Methodological framework for radiomics applications in Hodgkin Lymphoma
Authors: Sollini Martina , Kirienko Margarita , Cavinato Lara , Ricci Francesca , Biroli Matteo , Ieva Francesca , Calderoni Letizia , Tabacchi Elena , Nanni Cristina , Zinzani Pier Luigi , Fanti Stefano , Guidetti Anna , Alessi Alessandra , Corradini Paolo , Seregni Ettore , Stella Carmelo Carlo , Chiti Arturo 
Published: March 2020

Abstract
Background According to published data radiomics features differ between lesions of refractory/relapsing HL patients from those of long-term responders. However, several methodological aspects have not been elucidated yet.Purpose The study aimed at setting up a methodological framework in radiomics applications in Hodgkin Lymphoma (HL), especially at: a) developing a novel feature selection approach; b) evaluating radiomic intra-patient lesions’ similarity; c) classifying relapsing refractory (R/R) vs non-(R/R) patients.Methods We retrospectively included 85 patients (male:female=52:33; median age 35 years, range 19-74). LIFEx (www.lifexsoft.org) was used for [18F]FDG-PET/CT segmentation and feature extraction. Features were a-priori selected if they were highly correlated or uncorrelated to the volume. Principal component analysis-transformed features were used to build the fingerprints, that were tested to assess lesions’ similarity, using the silhouette. For intra-patient similarity analysis, we used patients having multiple lesions only. To classify patients as non-R/R and R/R, the fingerprint considering one single lesion (fingerprint_One) and all lesions (fingerprint_All) was tested using Random Undersamplig Boosting of Tree Ensemble (RUBTE). Results HL fingerprints included up to 15 features. Intra-patient lesion similarity analysis resulted in mean/median silhouette values below 0.5 (low similarity especially in the non-R/R group). In the test set, the fingerprint_One classification accuracy was 62% (78% sensitivity and 53% specificity); the classification by RUBTE using fingerprint_All resulted in 82% accuracy (70% sensitivity and 88% specificity). Conclusions Lesion similarity analysis was developed, and it allowed to demonstrate that HL lesions were not homogeneous within patients in terms of radiomics signature. Therefore, a random target lesion selection should not be adopted for radiomics applications. Moreover, the classifier to predict R/R vs non-R/R, performed the best when all the lesions were used.
The role of ICOSL in children with neutrophilic asthma
Authors: Dong Heting , JIANG Wujun , HUANG Li , WANG Meijuan , YAN Yongdong , HAO Chuangli , ZHANG Xinxing , GU Wenjing , SHAO Xuejun , Chen Zhengrong , Ji Wei 
Published: March 2020

Abstract
Background: It has been shown that certain severe and refractory asthma cases are due to neutrophil and not eosinophil infiltration. ICOSL (Inducible costimulatory molecular ligand) expression is closely associated with tumor and autoimmune diseases, while a limited amount of data has been published regarding the significance of ICOSL in children with neutrophilic asthma. The present study aimed to explore the abnormal expression of ICOSL in peripheral blood and bronchoalveolar lavage fluid (BALF) samples of children with neutrophilic asthma and their clinical significance. Methods: The present clinical study selected children who met the diagnostic criteria of asthma from the children's Hospital of Suchow University and excluded the patients with positive etiology. The children who were admitted to the hospital for foreign body inhalation in the same period were collected as the control group. The children with more than 50% (the percentage of neutrophils in BALF was 95% of the percentile in the control group) of neutrophils in BALF samples were assigned to the neutrophilic asthma group (NA group), whereas the remaining subjects comprised the asthma group (A group). The expression levels of ICOSL, IL-4, IL-17, IFN-, neutrophil elastase (NE) and matrix metalloproteinase (MMP)-9 were detected in plasma and BALF samples by enzyme-linked immunosorbent assays in order to analyze the differences in the levels of cytokines and clinical characteristics between children with neutrophilic asthma and non-neutrophilic asthma. Moreover, the potential mechanism of ICOSL in neutrophilic asthma was explored. Results: In strict accordance with the diagnostic criteria of asthma and following exclusion of pathogenic positive children, 32 children were finally enrolled, including 12 children in the NA group and 20 children in the A group. The mean hospitalization time of the NA group was longer than that of the A group (P<0.05). The concentration levels of ICOSL, IL-17, NE and MMP9 of the NA group in plasma and BALF samples were higher than those in the A group, while the levels of IFN-γ exhibited the opposite trend. A significant correlation was evident between ICOSL and IL-17 levels in plasma（r=0.753，P=0.012） and BALF（r=0.774，P=0.009）samples of the NA group. Conclusion: Children with asthma exhibited an immunity imbalance of Th1/Th2/Th17, whereas neutrophilic asthma children were more severely affected. The clinical treatment was considerably difficult and the hospitalization time was longer. ICOSL may regulate the secretion of IL-17 by Th17 and increase the levels of NE and MMP9, which are involved in the development of immune inflammation in neutrophils.
Whole-exome sequencing identifies GPSM1 as a susceptibility gene for premature ovarian insufficiency
Authors: Cai Xuzi , Fu Huijiao , Wang Yan , Liu Qiwen , Wang Xuefeng 
Published: March 2020

Abstract
Background Genetic causes of premature ovarian insufficiency (POI) account for approximately 20~25% of patients. So far, only a few genes have been identified. Results Here, we first identified the c.1840C>A on G-protein signaling modulator 1 (GPSM1) as a susceptibility locus for POI in 10 sporadic POI patients by whole-exome sequencing. The frequency of GPSM1 c.1840C>A was then verified as 3/20 in a POI sample of 20 patients (including the above 10 patients) by Sanger sequencing. RT-PCR and western blot analysis showed the expression of GPSM1 in rat ovaries was increased in the large antral follicle stage compared to the primordial follicle stage (P<0.01). The cell proliferation assay (CCK8) and flow cytometry suggested that the small-interfering RNA-induced silencing of Gpsm1 significantly increased apoptosis and decreased proliferation of rat ovarian granulosa cells (GCs) (P<0.01). Furthermore, suppression of Gpsm1 in GCs reduced levels of cAMP, PKAc, p-CREB as well as the ratio of Bcl-2/Bax, and increased the expression of Caspase-3 and Cleaved Caspase-3 (P<0.01). Conclusions In summary, this study identified a susceptibility variant GPSM1 c.1840C>A of POI for the first time. Gpsm1 was related to rat follicle development, and silencing increased apoptosis and decreased proliferation in rat GCs, possibly through inhibition of the cAMP-PKA-CREB pathway. These findings facilitate the development of the early molecular diagnosis of POI.