bluuebunny/crossref_metadata_2025_split huggingface dataset:
Remote monitoring and treatment of children and adolescents with type 1 diabetes
Authors: Laptev Dmitry N. , Emelyanov Andrey O. , Samoilova Yulia G. , Khramova Elena B. , Petriaikina Elena E. , Rybkina Irina G. , Filimonova Alla Yu. , Peterkova Valentina A. 
Published: December 2020

RATIONALE: Continuous subcutaneous insulin infusion (CSII) is an effective method for optimizing glycemic control in children with type 1 diabetes mellitus (DM1). However, the use of CSII does not always result in adequate glycemic control. Telehealth can be applied as one of the methods to improve the effectiveness of treatment.AIMS: To evaluate the use of remote medical support of children and adolescents with DM1 and its influence on glycemic control, quality of life, and incidence of acute complications of DM1.MATERIALS AND METHODS: We conducted a 24-week multi-institutional prospective open-label controlled clinical trial. 180 children and adolescents were included in this study and divided into the following categories: 1) age 8–18 years; 2) DM1 at least 1 year; 3) pump insulin therapy Medtronic Paradigm (Medtronic MiniMed, USA) at least 6 months; 4) self-monitoring of glycemia at least 4 times a day and replacement of the insulin pump infusion system at least once every 3 days; 5) inadequate glycemic control of DM1: the level of glycated hemoglobin (HbA1c) 7.5% or higher. Patients were assigned to a remote consultation group (RC; n=100) or a traditional control group (TC; n=80). All patients were trained on the basic principles of DM1 and CSII, and we measured initial HbA1c, then after 12 and 24 weeks, also registered and analyzed glycemic indicators and daily doses of insulin, evaluated and corrected the treatment. Patients or their parents in the RC group sent pump data via the Internet to the pump insulin therapy center at least once every 2 weeks at home and received treatment recommendations in response.RESULTS: The total number of patients included in the study in all institutions was 180 children at 8–18 years. Patients in both groups did not differ in age, gender, duration of DM1 and CSII, and HbA1c level. The total amount of remote consultations for all institutions was 949. The decrease in the level of HbA1c by the end of the study against the initial one was statistically significantly greater in the RC group: 1.17% compared to 0.59% in the TC group (p<0.05). The proportion of patients who reached the target level of HbA1c (<7.5%) was significantly higher in the RC group (32%) compared to the TC group (12.5%, p<0.05). During the study, the incidence of DKA and severe hypoglycemia in the RC group was statistically significantly lower.CONCLUSIONS: Remote monitoring in children with DM1 resulted in significant improvements in glycemic control (HbA1c, glycemic variability, and hypoglycemic frequency). The accumulation of evidence on the effectiveness and safety of telehealth in DM should contribute to implementing this approach in practical health care.
BENEFÍCIOS DO RESVERATROL COMO ATIVO COSMÉTICO NA PREVENÇÃO DO ENVELHECIMENTO CUTÂNEO
Authors: Paladini Andreia Medeiros , Lopes Talita Durante , Machado Karina Elisa 
Published: December 2020

A melhora dos parâmetros de saúde da população é um dos maiores feitos da humanidade e essa conquista se transformou em um desafio para os tempos de hoje. Viver mais é importante, desde que possamos adicionar qualidade de vida, física e mental, para os anos que ganhamos, e por isso a busca por recursos que possam nos ajudam a atingir esse objetivo tem estimulado novas pesquisas e dentre essas, destaca-se a procura de ativos a serem utilizados em formulações cosméticas, que possam combater o envelhecimento cutâneo. O envelhecimento da pele pode ser intrínseco, ou seja, aquele decorrente da passagem natural do tempo e da genética do corpo, ou pode ser extrínseco, aquele provocado por fatores ambientais, que interagem com a pele, e muitos desses fatores estão relacionados à produção de radicais livres, como tabagismo, consumo exagerado de álcool e estresse e, nesse contexto, um dos ativos que nos chama a atenção, no combate ao envelhecimento cutâneo, é o resveratrol. O resveratrol é um composto fenólico, encontrado em várias plantas e alimentos como o amendoim, uva e seus derivados, que exerce uma variedade de atividades biológicas como antioxidantes, anti-inflamatórias, cardioprotetoras, quimiopreventivas e neuroprotetoras. O presente artigo é caracterizado como sendo uma pesquisa bibliográfica do tipo descritiva com abordagem qualitativa e que tem como objetivo realizar uma revisão bibliográfica atualizada sobre o resveratrol e seu uso na prevenção do envelhecimento cutâneo. O resveratrol tem ação antioxidante e é ativador da sirtuína 1, que por sua vez, ativa proteínas reparadoras do DNA, podendo assim ser auxiliar no tratamento antienvelhecimento.
QUALIDADE MICROBIOLÓGICA DE QUEIJOS TIPO MINAS COMERCIALIZADOS EM BELO HORIZONTE, MG, BRASIL
Authors: Lopes Vanessa Cristina , Guedes Elika Karina , Candioto Marcus Vinicius Cossenzo , Delvivo Fernanda Meneghello , Lima Adriene Ribeiro 
Published: December 2020

O objetivo deste estudo foi avaliar a qualidade microbiológica de queijos Minas industrializados e artesanais comercializados em Belo Horizonte, MG. Foram coletadas 18 amostras de queijos, sendo nove do tipo Minas frescal, industrializados comercializados em supermercados; e nove do tipo Minas artesanal, comercializados no Mercado Municipal. A temperatura dos queijos amostrados foi aferida durante a fase de exposição para venda. A qualidade higiênico-sanitária dos queijos foi avaliada por meio da contagem de coliformes a 45 °C, Staphylococcus coagulase-positiva e pesquisa de Salmonella sp. Oito (88,9 %) amostras de queijo Minas frescal industrializado se encontravam em temperaturas acima do permitido no mercado varejista, e 100% das amostras de queijo artesanal estavam expostas a temperaturas inadequadas nas lojas do Mercado Municipal. Duas (22 %) amostras de queijo Minas industrializado e quatro (44 %) amostras de queijo Minas artesanal apresentaram contagens superiores ao preconizado pela legislação para coliformes a 45 °C. Em relação à contagem de Staphylococcus coagulase-positiva 100 % das amostras, tanto de queijo industrializado quanto de queijo artesanal, apresentaram contagens superiores aos padrões legais. Nenhuma amostra apresentou contaminação pela bactéria Salmonella sp. Dessa forma, a qualidade higiênico-sanitária de 100 % dos queijos Minas avaliados foi insatisfatória.
ANÁLISE DA SEGURANÇA DO USO DE IMUNOSSUPRESSORES POR PACIENTES INSUFICIENTES RENAIS
Authors: Toaldo Fernanda , Dos Santos Flavia Lorenzen , Sales Fernanda Cristina Ostrovski , Aschenbrenner Jaqueline de Jesus 
Published: December 2020

A insuficiência renal aguda (IRA) e a insuficiência renal crônica (IRC) consistem em patologias com incidência crescente ao longo dos últimos anos no Brasil. Segundo a Sociedade Brasileira de Nefrologia (2018), o número de pacientes com estas comorbidades tem aumentado cerca de 200% em 16 anos, tendo como alternativa de tratamento o transplante do órgão. Uma das principais terapias pós-transplante renal consiste no uso de imunossupressores, com o objetivo de conter a rejeição do enxerto. Em contrapartida, a segurança desta farmacoterapia ainda é bastante discutida, pela alta probabilidade de ocorrência de reações adversas. Esse estudo objetivou apresentar e analisar dados numéricos referentes à ocorrência de Reação Adversa a Medicamentos (RAM) relacionados a imunossupressores utilizados como farmacoterapia por pacientes com IRA ou IRC, internados em um Hospital Universitário do Município de Curitiba. PR. Trata-se de um estudo retrospectivo observacional, baseado na análise de prontuários físicos e eletrônicos de pacientes insuficientes renais agudos ou crônicos, por meio de busca ativa em tabelas de dispensação de imunossupressores. Este trabalho incluiu prontuários de 162 pacientes, sendo que 118 destes apresentaram uma ou mais possíveis reações adversas de imunossupressores. A RAM de maior prevalência no estudo foi “infecção oportunista” e a de menor prevalência “hipoalbuminemia”. Para maior confiabilidade dos resultados foi aplicado Algoritmo de Naranjo, onde “infecção” permaneceu relevante em 26% dos seus casos classificada pelo algoritmo como “provável”. Diante dos resultados expostos, observa-se a importância do farmacêutico clínico na otimização da farmacoterapia imunossupressora utilizada por pacientes insuficientes renais do hospital em questão.
Relationship between TIM-3 gene polymorphisms and steroid-resistant primary nephrotic syndrome in children
Authors: Lei Jing , Ma Songdong 
Published: December 2020

Nephrotic syndrome, also known as nephrosis, is a collection of symptoms in medicine and urology caused by damage to the basement membrane of the kidney glomeruli and the kidneys excrete a large amount of protein. This experiment was carried out to investigate the association of three single nucleotide polymorphisms (SNPs) of T-cell immunoglobulin and mucin-domain-containing-3 (Tim-3) with childhood primary nephrotic syndrome (PNS) steroid response in Han Chinese. For this purpose, a total of 218 children with steroid-resistant PNS and 189 children with steroid-responsive PNS were enrolled in this case-control study. Three single nucleotide polymorphisms (SNPs) of the TIM-3 gene promoter region (rs4704853, rs1051746, and rs10053538) were analyzed by polymerase chain reaction (PCR) and restriction enzyme digestion. Results showed that there were 124 males and 94 females in the steroid-resistant PNS group and 114 males and 75 females in the steroid-responsive PNS group. The mean ages of the two groups were 7.9 years and 7.7 years, respectively. The distribution of alleles of Rs1051746 and Rs10053538 were significantly different between the steroid-resistant PNS group and the steroid-responsive PNS group (P-value = 0.047 and 0.012, respectively). The distribution of their genotypes was also significantly different between the steroid-resistant PNS group and the steroid-responsive PNS group (P-value = 0.044 and 0.010, respectively). Haplotype G-C-G was less frequent among steroid-resistant PNS children than the steroid-responsive PNS children (P = 0.015). There was no significant difference between the three SNPs of TIM-3 and the clinical features of these PNS children (P>0.05). It concluded that this study provided evidence showing that the polymorphisms of Rs1051746 and Rs10053538 at the TIM-3 gene were related to childhood PNS steroid response. This result provided fundamental support for future studies on the role of TIM-3 in pathogenesis and therapy of childhood PNS.
Fibrillin 2 gene knockdown inhibits invasion and migration of lung cancer cells
Authors: Hong Qiaojun , Li Rong , Zhang Yiyan , Gu Kangsheng 
Published: December 2020

To investigate the effect of Fibrillin 2 (FBN2) expression on the invasion and migration of lung cancer cells, and the underlying mechanism. Protein and mRNA expressions of FBN2 were assayed. The relationship between FBN2 protein expression and clinical characteristics of lung cancer patients was analyzed. Correlation between FBN2 expression level and patient survival time was analyzed. Moreover, the mRNA and protein expression of FBN2 in lung cancer cells and human normal lung epithelial cells were assayed. After constructing low-expressing FBN2 cells, the cell proliferation, clone formation, migration and invasion capabilities were tested. Lung cancer cells proliferation with low FBN2 expression in nude mice was measured with a nude mouse tumorigenic experiment. The mRNA and protein expressions of FBN2 in lung cancer tissues were significantly higher than those in para-cancerous tissues (p<0.05). FBN2 protein expression was significantly correlated with TNM stage, lymph node metastasis and histological type (p<0.05). Survival time was markedly reduced in patients with high FBN2 expression (p<0.001). The expressions of FBN2 mRNA and protein were markedly higher in lung cancer cells than in human normal lung epithelial cells. The proliferation, migration and invasion of lung cancer cells were significantly inhibited by FBN2 knockdown. The FBN2 knockdown significantly inhibited the protein expressions of p-FAK, p-MEK and p-ERK. FBN2 is highly expressed in lung cancer tissues, and as an oncogene, it affects the pathogenesis of lung cancer. The knockdown of the expression of FBN2 significantly inhibits the proliferation, invasion and migration abilities of lung cancer cells.
Active Transition of Fear Memory Phase from Reconsolidation to Extinction through ERK-Mediated Prevention of Reconsolidation
Authors: Fukushima Hotaka , Zhang Yue , Kida Satoshi 
Published: December 2020

The retrieval of fear memory induces two opposite memory process, i.e., reconsolidation and extinction. Brief retrieval induces reconsolidation to maintain or enhance fear memory, while prolonged retrieval extinguishes this memory. Although the mechanisms of reconsolidation and extinction have been investigated, it remains unknown how fear memory phases are switched from reconsolidation to extinction during memory retrieval. Here, we show that an extracellular signal-regulated kinase (ERK)-dependent memory transition process after retrieval regulates the switch of memory phases from reconsolidation to extinction by preventing induction of reconsolidation in an inhibitory avoidance (IA) task in male mice. First, the transition memory phase, which cancels the induction of reconsolidation, but is insufficient for the acquisition of extinction, was identified after reconsolidation, but before extinction phases. Second, the reconsolidation, transition, and extinction phases after memory retrieval showed distinct molecular and cellular signatures through cAMP responsive element binding protein (CREB) and ERK phosphorylation in the amygdala, hippocampus, and medial prefrontal cortex (mPFC). The reconsolidation phase showed increased CREB phosphorylation, while the extinction phase displayed several neural populations with various combinations of CREB and/or ERK phosphorylation, in these brain regions. Interestingly, the three memory phases, including the transition phase, showed transient ERK activation immediately after retrieval. Most importantly, the blockade of ERK in the amygdala, hippocampus, or mPFC at the transition memory phase disinhibited reconsolidation-induced enhancement of IA memory. These observations suggest that the ERK-signaling pathway actively regulates the transition of memory phase from reconsolidation to extinction and this process functions as a switch that cancels reconsolidation of fear memory.SIGNIFICANCE STATEMENTRetrieval of fear memory induces two opposite memory process; reconsolidation and extinction. Reconsolidation maintains/enhances fear memory, while extinction weakens fear memory. It remains unknown how memory phases are switched from reconsolidation to extinction during retrieval. Here, we identified an active memory transition process functioning as a switch that inhibits reconsolidation. This memory transition phase showed a transient increase of extracellular signal-regulated kinase (ERK) phosphorylation in the amygdala, hippocampus and medial prefrontal cortex (mPFC). Interestingly, inhibition of ERK in these regions at the transition phase disinhibited the reconsolidation-mediated enhancement of inhibitory avoidance (IA) memory. These findings suggest that the transition memory process actively regulates the switch of fear memory phases of fear memory by preventing induction of reconsolidation through the activation of the ERK-signaling pathway.
Chondroitinase and Antidepressants Promote Plasticity by Releasing TRKB from Dephosphorylating Control of PTPσ in Parvalbumin Neurons
Authors: Lesnikova Angelina , Casarotto Plinio Cabrera , Fred Senem Merve , Voipio Mikko , Winkel Frederike , Steinzeig Anna , Antila Hanna , Umemori Juzoh , Biojone Caroline , Castrén Eero 
Published: December 2020

Perineuronal nets (PNNs) are an extracellular matrix structure rich in chondroitin sulfate proteoglycans (CSPGs), which preferentially encase parvalbumin-containing (PV+) interneurons. PNNs restrict cortical network plasticity but the molecular mechanisms involved are unclear. We found that reactivation of ocular dominance plasticity in the adult visual cortex induced by chondroitinase ABC (chABC)-mediated PNN removal requires intact signaling by the neurotrophin receptor TRKB in PV+neurons. Additionally, we demonstrate that chABC increases TRKB phosphorylation (pTRKB), while PNN component aggrecan attenuates brain-derived neurotrophic factor (BDNF)-induced pTRKB in cortical neurons in culture. We further found that protein tyrosine phosphatase σ (PTPσ, PTPRS), receptor for CSPGs, interacts with TRKB and restricts TRKB phosphorylation. PTPσ deletion increases phosphorylation of TRKBin vitroandin vivoin male and female mice, and juvenile-like plasticity is retained in the visual cortex of adult PTPσ-deficient mice (PTPσ+/−). The antidepressant drug fluoxetine, which is known to promote TRKB phosphorylation and reopen critical period-like plasticity in the adult brain, disrupts the interaction between TRKB and PTPσ by binding to the transmembrane domain of TRKB. We propose that both chABC and fluoxetine reopen critical period-like plasticity in the adult visual cortex by promoting TRKB signaling in PV+neurons through inhibition of TRKB dephosphorylation by the PTPσ-CSPG complex.SIGNIFICANCE STATEMENTCritical period-like plasticity can be reactivated in the adult visual cortex through disruption of perineuronal nets (PNNs) by chondroitinase treatment, or by chronic antidepressant treatment. We now show that the effects of both chondroitinase and fluoxetine are mediated by the neurotrophin receptor TRKB in parvalbumin-containing (PV+) interneurons. We found that chondroitinase-induced visual cortical plasticity is dependent on TRKB in PV+neurons. Protein tyrosine phosphatase σ (PTPσ, PTPRS), a receptor for PNNs, interacts with TRKB and inhibits its phosphorylation, and chondroitinase treatment or deletion of PTPσ increases TRKB phosphorylation. Antidepressant fluoxetine disrupts the interaction between TRKB and PTPσ, thereby increasing TRKB phosphorylation. Thus, juvenile-like plasticity induced by both chondroitinase and antidepressant treatment is mediated by TRKB activation in PV+interneurons.
Oleuropein alleviates gestational diabetes mellitus by activating AMPK signaling
Authors: Zhang Zhiwei , Zhao Hui , Wang Aixia 
Published: December 2020

Background
Gestational diabetes mellitus (GDM) has a high incidence rate among pregnant women. The objective of the study was to assess the effect of plant-derived oleuropein in attenuating inflammatory and oxidative stress of GDM.

Methods
Oleuropein was administered to GDM mice at the doses of 5 or 10 mg/kg/day. Body weight, blood glucose, insulin and hepatic glycogen levels were recorded. To evaluate the effect of oleuropein in reducing oxidative stress, ELISA was used to measure the hepatic oxidative stress markers. The inflammation levels of GDM mice were evaluated by measuring serum levels of IL-6 and TNF-α by ELISA and mRNA levels of IL-1β, TNF-α and IL-6 by real-time PCR (RT-PCR). The AMP-activated protein kinase (AMPK) signaling pathway was assessed by Western blot. Gestational outcome was analyzed through comparing litter size and birth weight.

Results
Oleuropein attenuated the elevated body weight of GDM mice and efficiently reduced blood glucose, insulin and hepatic glycogen levels. Oxidative stress and inflammation were alleviated by oleuropein treatment. The AMPK signaling was activated by oleuropein in GDM mice. Gestational outcome was markedly improved by oleuropein treatment.

Conclusions
Our study suggests that oleuropein is effective in alleviating symptoms of GDM and improving gestational outcome in the mouse model. This effect is achieved by attenuating oxidative stress and inflammation, which is mediated by the activation of the AMPK signaling pathway.
Un acercamiento hacia la apropiación y consumo de Instagram por parte de jóvenes universitarios
Authors: Montero Corrales Laura 
Published: December 2020

Introducción
El estudio de las redes sociales entre los jóvenes se ha convertido en una línea de investigación en crecimiento. Aunque existen numerosas investigaciones al respecto, el estudio de la apropiación y consumo social de la tecnología nos permite determinar cómo los jóvenes utilizan plataformas como Instagram y las incorporan de distintas formas a su vida diaria.
Objetivo
Por ello esta investigación tiene como objetivo descubrir y examinar las prácticas de apropiación y consumo de Instagram por parte de los estudiantes universitarios.
Método
El presente artículo presenta los resultados de un estudio en el cual se aplicó un cuestionario a 210 estudiantes, con edades entre 18 y 24 años de la Facultad de Ciencias Sociales de la Universidad de Costa Rica.
Resultados
Los resultados indican que los estudiantes universitarios utilizan esta red social como herramienta de interacción social, documentación, expresión personal y mirar lo que los otros hacen, así como un espacio de interacción con usuarios, marcas e influencers.
Conclusiones
Según los datos existen una serie de formas y prácticas por medio de las cuales los jóvenes universitarios hacen uso de Instagram, en especial su interés por usuarios de tipo influencers, que permiten comprender sus procesos de socialización y consumo de información a través de redes sociales.”
A Universidade Federal da Fronteira Sul no cenário brasileiro: notas a partir de Darcy Ribeiro
Authors: Dos Santos Dionei Ruã , Pithan da Silva Sidinei , Pansera de Araujo Maria Cristina 
Published: December 2020

O artigo analisa os ideais fundantes de universidades influenciadas diretamente por Darcy Ribeiro bem como seus livros e textos, objetivando diagnosticar sua presença nas universidades da atualidade. O texto estabelece algumas possíveis relações com o projeto da Universidade Federal da Fronteira Sul e do curso Interdisciplinar em Educação do Campo, apontando possíveis marcas desse autor, ao mesmo tempo resgatando seus conceitos e atualizando-os em razão de inéditos campos de possibilidades, especialmente no que se refere ao ingresso e permanência, no Ensino Superior, dos povos historicamente excluídos da sociedade, como é o caso de indígenas e sujeitos do campo.
Palavras-chave: Educação do Campo, Projeto de Universidade, Educação Popular.

The Universidade Federal da Fronteira Sul in the Brazilian scenario: notes from Darcy Ribeiro
Abstract
The article analyzes the founding ideals of universities directly influenced by Darcy Ribeiro as well as his books and texts, aiming to diagnose their presence in universities today. The text establishes some possible relations with the project of the Universidade Federal da Fronteira Sul and the Interdisciplinary course in Rural Education, pointing out possible marks of this author, at the same time rescuing his concepts and updating them due to unprecedented fields of possibilities, especially in the which refers to the entry and permanence, in Higher Education, of peoples historically excluded from society, as is the case of indigenous and rural subjects.
Keywords: Rural Education, University Project, Popular Education.

La Universidade Federal da Fronteira Sul en el escenario brasileño: notas de Darcy Ribeiro
Resumen
El artículo analiza los ideales fundacionales de las universidades directamente influenciadas por Darcy Ribeiro así como sus libros y textos, con el objetivo de diagnosticar su presencia en las universidades de hoy. El texto establece algunas posibles relaciones con el proyecto de la Universidad Federal de Fronteira Sul y el curso Interdisciplinario en Educación Rural, señalando posibles marcas de este autor, al mismo tiempo rescatando sus conceptos y actualizándolos debido a campos de posibilidades sin precedentes, especialmente en el que se refiere al ingreso y permanencia, en la Educación Superior, de pueblos históricamente excluidos de la sociedad, como es el caso de los sujetos indígenas y rurales.
Palabras clave: Educación Rural, Proyecto Universitario, Educación Popular.