bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surgical options for symptomatic old osteoporotic vertebral compression fractures: A retrospective study of 238 cases
Authors: Xu Zhengwei , Hao Dingjun , Dong Liang , Yan Liang , He Baorong 
Published: December 2020

Abstract
Background: Symptomatic osteoporotic vertebral compression fractures (OVCF) are increasing, as are acute and chronic pain episodes and progressive spinal deformities. However, there are no clear surgical treatment criteria for patients with these different symptoms. Therefore, this study aims to explore the surgical approaches for the treatment of OVCF with different symptoms and evaluate the feasibility of these surgical approaches.Methods: We retrospectively analyzed 238 symptomatic OVCF patients who entered our hospital from June 2013 to 2016. According to clinical characteristics and imaging examinations, these patients were divided into I-V grades and their corresponding surgical methods were developed. I, old vertebral fracture with no apparent instability, vertebral augmentation; II, old vertebral fracture with local instability, posterior reduction fusion internal fixation; III, old fractures with spinal stenosis, posterior decompression and reduction fusion and internal fixation; IV, old vertebral fracture with kyphosis, posterior osteotomy with internal fixation and fusion; V, a mixture of the above types, posterior osteotomy (decompression) with internal fixation and fusion. Postoperative visual analog score (VAS), oswestry disability index (ODI) scores, sagittal index (SI) and ASIA grades of neurological function were observed.Results: All 238 patients were followed up for 12-38 months, with an average follow-up of 18.5 months. After graded surgery, the VAS score, ODI score, and vertebral sagittal index SI of 238 patients were significantly improved, and the difference between the last follow-up results and the preoperative comparison was statistically significant (P˂0.05). Besides, the postoperative ASIA grades of 16 patients with nerve injury were improved from 14 patients with preoperative grade C, 2 patients with grade D to 4 patients with postoperative grade D and 12 patients with postoperative grade E.Conclusion: In this study, we concluded that graded surgery could better treat symptomatic old OVCF and restore spinal stability. This provides clinical reference and guidance for the treatment of symptomatic old OVCF in the future.
Addressing the quality of paediatric primary care: Health worker and caregiver perspectives from a process evaluation of PACK Child, a health systems intervention in South Africa
Authors: Curran Robyn Leigh , Murdoch Jamie , Bachmann Max , Bateman Eric , Cornick Ruth , Picken Sandy , Simelane Makhosazana Lungile , Fairall Lara 
Published: December 2020

Abstract
Background: The WHO’s Integrated Management of Childhood Illness (IMCI) has resulted in progress in addressing infant and child mortality. However, unmet needs of children continue to present a burden upon primary healthcare services. The capacity of services and quality of care offered require greater support to address these needs by extending and integrating curative and preventive care for the child with a long-term health condition and the child older than 5, not prioritised in IMCI. In response to these needs, the PACK Child intervention was developed and piloted in October 2017- February 2019 in the Western Cape Province of South Africa. We report health worker and caregiver perspectives of the existing paediatric primary care context as well as the extent to which PACK Child functions to address perceived problems within the current local healthcare system. Methods: This process evaluation involved 52 individual interviews with caregivers, 10 focus group discussions with health workers, 3 individual interviews with trainers, and 31 training observations. Interviews and focus groups explored participants’ experiences of paediatric primary care, perspectives of the PACK Child intervention, and tensions with implementation in each context. Inductive thematic analysis was used to analyse verbatim interview and discussion transcripts.Results: Perspectives of caregivers and health workers suggest an institutionalised focus of paediatric primary care to treating children’s symptoms as acute episodic conditions. Health workers’ reports imply that this focus is perpetuated by interactions between contextual features such as, IMCI policy, documentation-driven consultations, overcrowded clinics and verticalised care. Whilst these contextual conditions constrained health workers’ ability to translate skills developed within PACK Child training into practice, the intervention initiated expanded care of children 0-13 years and those with long-term health conditions, enhanced professional competence, improved teamwork and referrals, streamlined triaging, and facilitated probing for psychosocial risk.ConclusionPACK Child appears to be catalysing paediatric primary care to address the broader needs of children, including long-term health conditions and the identification of psychosocial problems. However, to maximise this requires primary care to re-orientate from risk minimisation on the day of attendance towards a view of the child beyond the day of presentation at clinics.
mTOR-dependent dysregulation of autophagy contributes to the retinal ganglion cell loss in streptozotocin-induced diabetic retinopathy
Authors: Madrakhimov Sanjar Batirovich , Yang Jin Young , Kim Jin Ha , Han Jung Woo , Park Tae Kwann 
Published: December 2020

Abstract
Background: Neurodegeneration, an early event in the pathogenesis of diabetic retinopathy (DR), precedes clinically detectable microvascular damage. Autophagy dysregulation is considered a potential cause of neuronal cell loss, however underlying mechanisms remain unclear. The mechanistic target of rapamycin (mTOR) integrates diverse environmental signals to coordinate biological processes, including autophagy. Here, we investigated the role of mTOR signaling in neuronal cell death in diabetic retinopathy. Methods: Diabetes was induced by a single intraperitoneal injection of streptozotocin and tissue samples were harvested at 1, 2, 3, 4, and 6 months of diabetes. Early-stage of diabetic retinopathy was investigated in 1-month-diabetic mice treated with phlorizin (two daily subcutaneous injections at a dose of 200 mg/kg of body weight during the last 7 full days of the experiment and the morning of the 8th day, 3 h before sacrifice) or rapamycin (daily intraperitoneal injections, at a dose of 3mg/kg for the same period as for phlorizin treatment). The effect of autophagy modulation on retinal ganglion cells was investigated in 3-months-diabetic mice treated with phlorizin (two daily subcutaneous injections during the last 10 full days of the experiment and the morning of the 11th day, 3 h before sacrifice) or MHY1485 (daily i.p. injections, at a dose of 10 mg/kg for the same period as for phlorizin treatment). Tissue samples obtained from treated/untreated diabetic mice and age-matched controls were used for Western blot and histologic analysis.Results: mTOR-related proteins and glucose transporter 1 (GLUT1) was upregulated at 1 month and downregulated in the following period up to 6 months. Diabetes-induced neurodegeneration was characterized by an increase of apoptotic marker – cleaved caspase 3, a decrease of the total number of cells, and NeuN immunoreactivity in the ganglion cell layer (GCL), as well as an increase of autophagic protein. Insulin-independent glycemic control restored the mTOR pathway activity and GLUT1 expression, along with a decrease of autophagic and apoptotic proteins in 3-months-diabetic mice neuroretina. However, blockade of autophagy using MHY1485 resulted in a more protective effect on ganglion cells compared with phlorizin treatment. Conclusion: Collectively, our study describes the mechanisms of neurodegeneration through the hyperglycemia/ mTOR/ autophagy/ apoptosis pathway.
Generation of Inner Ear Sensory Neurons Using Blastocyst Complementation in a Neurog1+/- -Deficient Mouse&nbsp;
Authors: Steevens Aleta R. , Griesbach Matthew W. , You Yun , Dutton James R. , Low Walter C. , Santi Peter A. 
Published: December 2020

Abstract
This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/- heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.
Preemptive intravenous iron therapy versus autologous whole blood therapy for early postoperative hemoglobin level in patients undergoing bimaxillary orthognathic surgery: A prospective randomized noninferiority trial
Authors: Chae Min Suk , Lee Mihyun , Choi Min Ho , Park Je Uk , Park Misun , Kim Young Hoon , Choi Hoon , Joo Jin , Moon Young Eun 
Published: December 2020

Abstract
Background: Previous studies have reported the efficacy and safety of intravenous (IV) iron therapy during the perioperative period as an alternative and adjunct to allogeneic blood transfusion. Preemptive IV iron therapy provides noninferior hemoglobin levels on postoperative day (POD) 1 compared to autologous whole blood therapy (AWBT) in healthy patients who had undergone bimaxillary orthognathic surgery.Methods: This was a prospective, patient-randomized, noninferiority trial. After excluding 2 patients, 64 patients were divided into two groups: the IV iron therapy group (patients received IV iron infusion 4 weeks before surgery; n = 32) and the AWBT group (2 units of autologous whole blood were collected 4 and 2 weeks before surgery; n = 32). The primary outcome was hemoglobin level on POD 1 and the prespecified noninferiority limit was –1 g/dL. Results: Baseline data were comparable, including hemoglobin and iron levels, between the two groups. Immediately before surgery, the levels of hemoglobin, iron, and ferritin were higher in the IV iron group than in the AWBT group. The mean treatment difference (iron group – whole blood group) in hemoglobin level on POD 1 between the two groups was 0.09 (95% CI = –0.83 to 1.0). As the lower limit of the 95% CI (–0.83) was higher than the prespecified noninferiority margin (δ = –1), noninferiority was established. On POD 2, the hemoglobin level became lower in the iron group, which eventually led to greater requirement of allogeneic blood transfusion compared to the whole blood group. However, the iron group did not require allogeneic blood transfusion during or early after surgery, and the whole blood group showed continuously higher incidence of overt iron deficiency compared to the iron group. Conclusion: As collection of autologous whole blood caused overt iron loss and anemia before surgery and intraoperative transfusion of whole blood was not able to prevent the occurrence of persistent iron deficiency after surgery, IV iron therapy was found to have potential benefits for iron homeostasis and subsequent erythropoiesis in healthy patients early after bimaxillary orthognathic surgery.Trial registration: Clinical Research Information Service, Republic of Korea, approval number: KCT0003680 on March 27, 2019https://cris.nih.go.kr/cris/search/search_result_st01_kren.jsp?seq=15769&sLeft=2&ltype=my&rtype=my
Salting-Out Induced Liquid-Liquid Microextraction for Alogliptin Benzoate Determination in Human Plasma by HPLC/UV
Authors: Hammad Sherin Farouk , Abdallah Inas , Bedair Alaa , Mansour Fotouh 
Published: December 2020

Abstract
Salting-out induced liquid-liquid microextraction method has been developed for plasma sample treatment before determination of alogliptin by high performance liquid chromatography with UV detection. Several parameters were optimized to achieve maximum enrichment, including type of extractant, volume of extractant, type of anion, type of cation, salt amount and pH. The optimum conditions were attained using 500 µL of acetonitrile, added to 1 mL of aqueous sample containing 250 mg of sodium chloride at pH 12. An RP-HPLC method was developed and validated according to the International Conference on Harmonization guidelines M10. The method was linear in the concentration range of 0.1 to 50 µg /mL (correlation coefficient= 0.997). The limit of detection was 0.019 µg/mL and limit of quantitation was 0.06 µ g/mL. The method was accurate and precise with an average % recovery of 99.7% and a % relative standard deviation ranging between 1.5 and 2.5. These results showed that the salting-out induced liquid-liquid microextraction methods could be better than other sample preparation protocols in terms of sensitivity, easiness, solvent consumption and waste reduction.
CKD and CKDu in Northern Peru: a cross-sectional analysis under the DEGREE protocol
Authors: Ruiz-Alejos Andrea O. , Caplin Ben , Miranda J. Jaime , Pearce Neil , Bernabé-Ortiz Antonio 
Published: December 2020

Abstract
Background:This study estimated the prevalence and risk factors for decreased estimated glomerular filtration rate (eGFR) in those without known hypertension, type 2 diabetes mellitus, or heavy proteinuria as a surrogate marker for chronic kidney disease of unknown cause (CKDu) among adults in the North of Peru.Methods:A cross-sectional study was conducted following the Disadvantaged Populations eGFR Epidemiology (DEGREE) Study protocol. Low eGFR was defined based on a single eGFR ≤60 mL/min/1.7m2 estimated using the CKD-EPI equation. Environmental conditions related to CKDu (i.e. work in agriculture or sugarcane, water source, heat intolerance, and pesticide exposure) were evaluated, in addition to traditional risk factors for CKD (i.e. smoking, heavy drinking, physical activity, hypertension, type 2 diabetes mellitus, urolithiasis, among others).Results:A total of 1514 subjects were included in the study, mean age 45.1 (SD: 16.4), and 55.2% were females. Overall, only 26 cases (1.7%; 95%CI: 1.1% - 2.5%) had an eGFR <60mL/min/1.7m2 compatible with CKD definition; when those with hypertension and type-2 diabetes or heavy proteinuria were excluded, according to the DEGREE protocol, the estimate fell to 0.9% (95%CI: 0.4% - 1.5%). Low physical activity levels (OR = 1.99; 95%CI: 1.18 – 3.34), hypertension (OR = 2.07; 1.26 – 3.41), and urolithiasis (OR = 1.97; 95%CI: 1.18 – 3.27) were factors associated with low eGFR.Conclusions:A low population-based prevalence of low eGFR (as a surrogate for CKDu), both in rural and urban settings areas, in the Northern Peru, was found. Low physical activity levels, hypertension and urolithiasis were factors associated with low eGFR. Interventions to prevent CKD cases may be focused on well-known CV risk factors and urolithiasis.
Wiedza dotycząca zrównoważonego rozwoju wśród młodzieży licealnej na podstawie badań pilotażowych
Authors: Żeber-Dzikowska Ilona 
Published: December 2020

The balanced development is at present the challenge for creating the awareness not only of the particular society, but also in the widest sense for the whole generation of the people. Undertaking such issues, the role of education during all stages of teaching, should be taken into consideration, especially, in the range of propagating the idea of responsibility for the natural environment with eco-development. It is a specially important element of teaching according to the principle of pro-environmental education to undertake all actions in the educational centers, which aim is to stimulate the environmental awareness of young people as well as to prepare the pedagogical staff to the realization of the wide range of methods and strategies that allow to get the best results during the process of educating young generation.
The appropriate undertakings should be realized in order to support the education in the process of introducing the right value system and positive attitudes towards realization of the sixth Environmental politics of the EU Programme.
With the connection to the carried on initial research associated with the knowledge about the balanced development among high school students, analysing the results of the questionnaires, the answer to the question whether students possess any kind of knowledge on balanced development was to be learned.
Swoistość ludzkiej kultury - wampiryzm
Authors: Tomczyk Jacek , Dygudaj Anna 
Published: December 2020

Every myth, regardless of its nature, refers back to some event placed in illio tempore. By this fact it constitutes a pattern to all situations and activities in which the event may reappear, the myth can degrade into an epical legend, a ballad or a roman, but it can also survive in a limited form in superstitions, customs and longings without losing neither its structure nor its meaning. In the history of mankind, the perception contributed to the formation of culture - the total of artifacts, both material and immaterial (spiritual or symbolic), these achievements, characteristic for particular society, constitute a model of social behavior. While interpreting the external world people relied on supernatural explanations to some extent, which depended on the level of intellectual development. Initially many facts were explained with the interference of dark, demonic powers, adopting diverse forms in people’s imagination, thanks to such interpretation of reality the vampire was born to existence.
The vampire has undergone a peculiar evolution, the features of its character often changed and the figure was many times reborn in varied forms. Settled in present times, the silhouette of the vampire remains realistic in some people’s minds and has no tendency to modify. Its presence in modern times is mainly perceptible through the creation and development of vampire - worshiping sects.
The following thesis is aimed to present the evolution of beliefs and picture of the vampire, as well as ancient practices and ways of treating people suspected of vampirism, the text enables a close look into the structure and functioning of modern vampire-related cults, it also attempts to explain the phenomenon of vampirism and its continuously increasing popularity.
Poznawanie prawdy i zdolność przewidywania w naukach przyrodniczych
Authors: Grochowska Irena 
Published: December 2020

Representatives of natural sciences (biologists, physicists, chemists) are scientists who deal with the part of knowledge regarding aspects of natural reality. If we regard natural reality to cosmos we can say that the possibilities of direct research are possible only in a small area. In spite of the limitations of access researchers do not get lost in the chaos of already studied separate images, but the gained experience, together with evolution of human thought create more and more uniform image, despite of numerous missing pieces.
Natural reality is complex, complicated and difficult to familiarize. One of the main problems of studying the natural reality is its complexity and variety of dynamic processes which create this reality. Another difficulty is the systematized and uniform terminology and concepts used. In the days of fast developing new disciplines and atomization of science it is almost impossible.
Natural reality exists independent of our knowledge of it. However the awareness of the difficulties and limitations in studying it should help in being responsible for the process of acquiring knowledge. Acquiring knowledge should therefore mirror the mechanisms existing in nature, that is why it was necessary to discuss the dynamics of getting to know nature with indication to some definitions of truth and its kinds, as well as various research mistakes.
Next to getting to know the things themselves it is important in science to detect and eliminate the mistakes. It is necessary to know the criteria of acknowledging or denying the genuine and false nature of research results, hypothesis or new theories in order to properly acquire knowledge about the natural world and properly communicate the results of studying.
Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle
Authors: Irianingsih Sri Handayani , Poermadjaja Bagoes , Wuryastuti Hastari , Wasito Raden 
Published: December 2020

The bovine viral diarrhea virus (BVDV) is a major viral pathogen in cattle worldwide. In Indonesia, diversity in subgenotypes of BVDV-1 has been observed, with the highest proportion of subgenotype -1a, followed by -1c, -1b, and -1d. So far, phylogenetic analysis of BVDV-1 is based on nucleotide sequences of the 5′ UTR and partial NS5B regions. Accuracy in identifying the subgenotype and antigenic type is critical for vaccine development and effective vaccination. The aim of this study was to determine genetic recombination of BVDV through phylogenetic analysis of five different regions (5′ UTR, NPro, E2, NS3, and NS5B) of BVDV in persistently infected dairy cattle. Five isolates were sequenced using next-generation sequencing, and data were analyzed with the CLC Genomic Workbench 9.0 and MEGA-X programs. Phylogenetic analysis based on the 5′ UTR (275 nt), NPro (504 nt), E2 (1,122 nt), NS3 (2,049 nt), and NS5B (2,157 nt) regions indicated that one BVDV isolate from Banyumas, Central Java, could be classified into different subgenotypes based on the E2 region (-1c), but the same subgenotype based on the other four regions (-1a), suggesting the presence of genetic recombination of the BVDV subgenotypes -1a and -1c in persistently infected dairy cattle.
Antagonism Test of Trichoderma atroviride and Gliocladium sp. Bali Local Isolates As a Disease Control of Blendok Disease (Botryodiplodia theobromae) in Grapefruit (Citrus grandis L. Osbeck)
Authors: Kalimutu Putu Krisnawan , Komang Mahardika Ida Bagus , Anak Agung Sagung Putri Risa Adriani 
Published: December 2020

This study aims to determine the ability of Trichoderma atroviride and Gliocladium sp. in inhibiting the fungus that causes Gumosis disease Botryodiplodia theobromae. This study used a completely randomized design with two single treatments and was repeated eight times.
In the in-vitro antagonism test Trichoderma atroviride and Gliolcadium sp. can inhibit the fungus Botryodiplodia theobromae as well. The percentage of inhibition was 94.58% in the Trichoderma atroviride antagonism test and 81.67% in the Gliocladium sp. antagonism test. Whereas the Botryodiplodia theobromae Colony Area had no significant effect on both treatments. Each Botryodiplodia theobromae Colony area is 18.34 cm2 with T. atroviride treatments and 22.25 cm2 with Gliocladium sp. treatment. The growth rate of Botryodiplodia Theobroma fungi was superior to that of Trichoderma atroviride and Gliocladium sp.