bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inferring the votes in a new political landscape. The case of the 2019 Spanish Presidential elections.
Authors: Grimaldi Didier , Diaz Javier , Arboleda Hugo 
Published: July 2020

Abstract
The avalanche of personal and social data circulating in Online Social Networks over the past 10 years has attracted a great deal of interest from Scholars and Practitioners who seek to analyse not only their value, but also their limits. Predicting election results using Twitter data is an example of how data can directly influence the politic domain and it also serves an appealing research topic. This article aims to predict the results of the 2019 Spanish Presidential election and the voting share of each candidate, using Tweeter. The method combines sentiment analysis and volume information and compares the performance of five Machine Learning algorithms. Several data scrutiny uncertainties arose that hindered the prediction of the outcome. Consequently, the method develops a political lexicon-based framework to measure the sentiments of online users. Indeed, an accurate understanding of the contextual content of the tweets posted was vital in this work. Our results correctly ranked the candidates and determined the winner by means of a better prediction of votes than official research institutes.
iNOS is not responsible for nitrosylation of RyR1 in mdx mice with truncated dystrophin
Authors: Nogami Ken'ichiro , Maruyama Yusuke , Elhussieny Ahmed , Sakai-Takemura Fusako , Tanihata Jun , Kira Jun-ichi , Miyagoe-Suzuki Yuko , Takeda Shin'ichi 
Published: July 2020

Abstract
Background: Previous research indicated that nitric oxide synthase (NOS) is the key molecule for S-nitrosylation of ryanodine receptor 1 (RyR1) in DMD model mice (mdx mice) and that both neuronal NOS (nNOS) and inducible NOS (iNOS) might contribute to the reaction because nNOS is mislocalized in the cytoplasm and iNOS expression is higher in mdx mice. We investigated the effect of iNOS on RyR1 S-nitrosylation in mdx mice and whether transgenic expression of truncated dystrophin reduced iNOS expression in mdx mice or not.Methods: Three- to 4-month-old C57BL/6J, mdx, and transgenic mdx mice expressing exon 45-55-deleted human dystrophin (Tg/mdx mice) were used. We also generated two double mutant mice, mdx iNOS KO and Tg/mdx iNOS KO to reveal the iNOS contribution to RyR1 S-nitrosylation. nNOS and iNOS expression levels in skeletal muscle of these mice were assessed by immunohistochemistry (IHC), qRT-PCR, and Western blotting. Total NOS activity was measured by a citrulline assay. A biotin-switch method was used for detection of RyR1 S-nitrosylation. Statistical differences were assessed by one-way ANOVA with Tukey-Kramer post-hoc analysis.Results: mdx and mdx iNOS KO mice showed the same level of RyR1 S-nitrosylation. Total NOS activity was not changed in mdx iNOS KO mice compared with mdx mice. iNOS expression was undetectable in Tg/mdx mice expressing exon 45-55-deleted human dystrophin, but the level of RyR1 S-nitrosylation was the same in mdx and Tg/mdx mice.Conclusion: Similar levels of RyR1 S-nitrosylation and total NOS activity in mdx and mdx iNOS KO demonstrated that the proportion of iNOS in total NOS activity was low, even in mdx mice. Exon 45-55-deleted dystrophin reduced the expression level of iNOS, but it did not correct the RyR1 S-nitrosylation. These results indicate that iNOS was not involved in RyR1 S-nitrosylation in mdx and Tg/mdx mice muscles.
HTLV screening of blood donors using chemiluminescence immunoassay in three major provincial blood centers of China
Authors: Zhao Junpeng , Zhao Feixue , Han Wei , Xu Xiaoxuan , Wang Lilin , Li Ran , Li Tong , Wu Linfeng , Du Dandan , Zeng Xuezhen , Cui Xiujuan , Chen Yunlong , Zeng Jinfeng , Wang Lunan 
Published: July 2020

Abstract
Background: Human T-cell lymphotropic virus (HTLV) remains a major safety concern for blood supplies. Despite many HTLV positive cases being reported in southeastern China, the detection of HTLV has not been prioritized in routine blood screening. Additionally, data on the prevalence of HTLV infection among blood donors is also limited.Objective: The objective of this study was to investigate the prevalence of HTLV among blood donors in three Chinese provinces through their representative blood centers, to evaluate the feasibility of chemiluminescence immunoassay (CLIA) for blood screening.Methods: From November 2018 to March 2019, blood plasma samples were collected from Hebei, Changsha, and Shenzhen blood centers and were screened for the HTLV-1/2 antibody using a CLIA and enzyme-linked immunosorbent assay (ELISA). This was followed by confirmatory tests using INNO-LIA HTLV I/II.Results: A total of 59,929 blood donations were collected and screened for HTLV-1/2. The reactive rate of CLIA and ELISA among donations in the Shenzhen blood center (0.0943%, 27/28, 621) was higher than Hebei (0.0248%, 4/16,144), and Changsha (0.0198%, 3/15,164) (p < 0.05). After confirmation, 3 samples were confirmed as indeterminate for HTLV antibodies, and only one sample from the Shenzhen blood center was confirmed as HTLV-1. The overall prevalence of HTLV-1/2 was 1.67 per 100 000 (1/59 929). The HTLV-infected blood came from a 32-year-old first-time female donor with a high school degree, who belonged to the SHE ethnic minority and was born in the Fujian province.Conclusion: In summary, the overall prevalence of HTLV-1/2 among blood donors in the three blood centers in China remains relatively low. However, blood donations with positive or indeterminate results for HTLV antibodies reminded us of the importance of HTLV screening among blood donors in China.
Variability and Change Over Time of Weight and BMI Among Adolescents and Adults with Prader-Willi Syndrome: A 6-month text-based observational study
Authors: Vrana-Diaz Caroline , Balasubramanian Priya , Kayadjanian Nathalie , Bohonowych Jessica , Strong Theresa V. 
Published: July 2020

Abstract
Background: Prader-Willi syndrome (PWS) is a rare neurodevelopmental disorder in which hyperphagia (excessive appetite) is a hallmark feature. Understanding how weight changes over time in this population is important for capturing the contemporary natural history of the disorder as well as assessing the impact of new treatments for hyperphagia. Therefore, we aimed to determine the feasibility of a remote assessment of weight change over time in PWS. Methods: We developed a text message-based, prospective cohort study of adolescents and adults with PWS to assess changes in weight and body mass index (BMI) over a six-month period. Weight was collected weekly, while changes in height, living situation, access to food, activity level, and medication were collected at three-month intervals. Results: One hundred and sixty-five participants enrolled in the study, with a mean age of 19.7 years (range 12 - 48). There was considerable variability in weight across participants (range: 76.8 – 207.7 kg). Thirty-three percent of the participants were normal weight, while 15% were overweight and 52% were obese. Overall, the weight of the study participants increased over the study period (mean weight change +2.35%), while BMI was relatively stable, albeit high (mean BMI of 31.4 at baseline, mean BMI percent change + 1.42%). Changes in living situation, activity, food access, and medication had limited impact on weight and BMI changes. Multivariable analysis found that time, sex, age, and percentage of life on growth hormone (GH) therapy were statistically significant fixed effects. Participants submitted more than 95% of possible weight data points across the 26 weeks of the study. Conclusions: This remote, observational study of weight change in PWS showed small increases in weight and BMI over a six-month period. Participants were highly compliant with this text message-based study, suggesting that mobile technology-based data collection was manageable for the participants. We anticipate that the results of this study will inform clinical trials for hyperphagia/obesity related therapies in PWS and provide a basis for understanding the efficacy of new therapies for hyperphagia in the real-world setting.
Fabrication and electrical characteristics of flash-sintered SiO2-doped ZnO-Bi2O3-MnO2 varistors
Authors: Peng Pai , Deng Yujun , Niu Jingpeng , Shi Liyi , Mei Yunzhu , Du Sanming , Liu Juan , XU Dong 
Published: July 2020

Abstract
The dense ZnO-Bi2O3-MnO2-xSiO2 (ZBMS) varistors for x= 0, 1, 2, 3 wt.% were fabricated by flash sintering method under the low temperature of 850 ℃ within 2 minutes. The sample temperature was estimated by a black body radiation model in the flash sintering process. The crystalline phase assemblage, density, microstructure, and electrical characteristics of the flash-sintered ZBMS varistors with different SiO2 doped content were investigated. According to the XRD analysis, many secondary phases were detected due to the SiO2 doping. Meanwhile, the average grain size decrease with increasing SiO2-doped content. The improved nonlinear characteristics were obtained in SiO2-doped samples, which can be attributed to the ion migration and oxygen absorption induced by the doped SiO2. The flash-sintered ZBMS varistor ceramics for x=2 wt.% exhibited excellent comprehensive electrical properties, with the nonlinear coefficient of 24.5, the threshold voltage and leakage current of 385 V×mm-1 and 11.8 µA, respectively.
Indocyanine Green Fluorescence-guided intraoperative detection of peritoneal carcinomatosis: systematic review.
Authors: Baiocchi Gian Luca , Gheza Federico , Molfino Sarah , Arru Luca , Vaira Marco , Giacopuzzi Simone 
Published: July 2020

Abstract
BACKGROUND. To review the available clinical data about the value of Indocyanine Green (ICG) fluorescence imaging for intraoperative detection of peritoneal carcinomatosis. METHODS. We conducted a systematic review, according to the PRISMA guidelines, for clinical series investigating the possible role of ICG fluorescence imaging in detecting peritoneal carcinomatosis during surgical treatment of abdominal malignancies. With the aim to analyze actual application in the daily clinical practice, papers including trials with fluorophores other than ICG, in vitro and animals series were excluded. Data on patients and cancer features, timing, dose and modality of ICG administration, sensitivity, specificity and accuracy of fluorescence diagnosis of peritoneal nodules were extracted and analyzed. RESULTS. Out of 192 screened papers, we finally retrieved 7 series reporting ICG-guided detection of peritoneal carcinomatosis. Two papers reported the same cases, thus only 6 series were analyzed, for a total of 71 patients and 353 peritoneal nodules. The investigated tumors were colorectal carcinomas in 28 cases, hepatocellular carcinoma in 16 cases, ovarian cancer in 26 cases and endometrial cancer in 1 case. In all but 4 cases, the clinical setting was an elective intervention in patients known as having peritoneal carcinomatosis. No series reported a laparoscopic procedure. Technical data of ICG management were consistent across the studies. Overall, 353 lesions were harvested and singularly evaluated. Sensitivity varied from 72.4% to 100%, specificity from 54.2% to 100%. Two series reported that planned intervention changed in 25% and 29% of patients, respectively. CONCLUSION. Indocyanine Green based fluorescence of peritoneal carcinomatosis is a promising intraoperative tool for detection and characterization of peritoneal nodules in patients with colorectal, hepatocellular, ovarian carcinomas. Further prospective studies are needed to fix its actual diagnostic value on these and other abdominal malignancies with frequent spread to peritoneum.
In vivo length change of ligaments of normal knees during dynamic high flexion
Authors: Kono Kenichi , Konda Shoji , Yamazaki Takaharu , Tanaka Sakae , Sugamoto Kazuomi , Tomita Tetsuya 
Published: July 2020

Abstract
Background Few studies compared the length change of ligaments of normal knees during dynamic activities of daily living. The aim of this study was to investigate in vivo length change of ligaments of the normal knees during high flexion. Methods Eight normal knees were investigated. Each volunteer performed squatting, kneeling, and cross-leg motions. Each sequential motion was performed under fluoroscopic surveillance in the sagittal plane. The femoral, tibial, and fibular attachment areas of the anterior cruciate ligament (ACL), posterior cruciate ligament (PCL), deep medial collateral ligament (dMCL), superficial medial collateral ligament (sMCL), and lateral collateral ligament (LCL) were determined according to osseous landmarks. After 2D/3D registration, the direct distance from the femoral attachment to the tibial or fibular attachment was measured as the ligament length. Results From 20° to 90° with flexion, the ACL was significantly shorter during cross-leg motion than during squatting. For the PCL, dMCL, sMCL, and LCL, there were no significant differences among the 3 motions. Conclusion The ACL was shorter during cross-leg motion than during squatting in mid-flexion. This suggests that the ACL is looser during cross-leg motion than during squatting. On the other hand, the length change of the PCL, MCL, and LCL did not change even though the high flexion motions were different.
Association of MTHFR C677T polymorphism with severity and localization of chronic atrophic gastritis patients without Helicobacter pylori infection : a case control study
Authors: Kong Siya , Ye Feng , Dang Yini , Hua Yifei , Zhang Guoxin 
Published: July 2020

Abstract
Background: Previous reports indicate that the methylenetetrahydrofolate reductase (MTHFR) 677C>T polymorphism plays a role in gastric cancer. However, whether it influences the development and progression of atrophic gastritis remains unclear. We aimed to determine the possible association between the MTHFR 677C>T polymorphism and the severity of atrophic gastritis.Methods: A total 128 patients without Helicobacter pylori infection were included in the study. The presence and severity of gastric atrophy was assessed by histology using OLGA and OLGIM Gastritis Staging System. MTHFR 677C>T genotyping was performed by digital fluorescence molecular hybridization. Categorical variables were analyzed by percentages using the χ2 test.Results: In this study, the TT genotype was significantly more frequent among Helicobacter pylori-negative patients aged ≤44 years (age ≤44 years vs. >44 years, P=0.039). Patients with the TT genotype showed a higher ratio of incisura with atrophy or intestinal metaplasia (TT vs. CC+CT, P=0.02). Furthermore, the TT genotype was associated with more severe lesions compared with the CC+CT genotypes (TT vs. CC+CT for atrophy: P=0.07; for intestinal metaplasia: P=0.01; for moderate-to-severe lesions: P=0.01). OLGA and OLGIM stages III-IV were observed more frequently in patients with the TT genotype compared with the CC+CT genotypes (for OLGA: P=0.003; for OLGIM: P=0.036).Conclusions: The MTHFR 677C>T TT genotype showed an increased risk of moderate-to-severe lesions by OLGA and OLGIM stages, and these results suggest that the MTHFR C677T polymorphism may serve as a predictive marker for precancerous gastric lesions, especially in Helicobacter pylori-negative patients aged ≤44 years.
Prevalences and Associated Factors of Electrocardiographic Abnormalities in Chinese Adults: A Cross-sectional Study
Authors: Yu Liping , Ye Xiaojun , Yang Zhaojun , Yang Wenying , Zhang Bo 
Published: July 2020

Abstract
Background: Electrocardiogram (ECG) is widely used to screen cardiac diseases. To date, no large population study has provided estimates of the prevalences of ECG findings in China. We aim to investigate the prevalences and associated factors of ECG abnormalities in a general population of Chinese adults. Methods: ECG data were obtained from 34,965 participants in the 2007-2008 China National Diabetes and Metabolic Disorders Study. ECG abnormalities were classified according to the Minnesota coding (MC) criteria. Prevalences of variant ECG abnormalities were calculated. The associations between ECG abnormalities and gender, age and other risk factors for cardiovascular diseases (CVD) were analyzed by multivariate logistic regression test. Results: The prevalences of major arrhythmias were 1.70%, 2.37% and 1.04% in the whole population, men and women, respectively. Atrial fibrillation/flutter was found in 0.35% of men and 0.20% of women. ST depression and T abnormalities accounted for 10.96%, 7.54% and 14.32% in the whole population, men and women, respectively. Independent of gender and other CVD risk factors, older age significantly increased the odds of having atrial fibrillation/flutter, complete left bundle branch block, complete right bundle branch block, sinus tachycardia, atrial/junctional/ventricular premature beats, ST depression and T abnormalities, tall R wave left, left/right atrial hypertrophy, left axis deviation and low voltage. Hypertension, overweight, obesity and hypercholesterolemia all independently increased the odds of having ST depression and T abnormalities. History of cardiovascular/cerebrovascular diseases was positively associated with major arrhythmias, ST depression and T abnormalities and tall R wave left. Conclusions: This study provides estimates of the prevalences of ECG findings in a large population of Chinese adults. Gender, age, CVD risk factors and history of cardiovascular/cerebrovascular diseases were significantly associated with ECG abnormalities.
Genetic Polymorphisms of PGF and TNFAIP2 Genes Related to Cervical Cancer Risk Among Uygur Females from China
Authors: Ainiwaer Zumurelaiti , Maisaidi Reyilanmu , Liu Jing , Han Lili , Husaiyin Sulaiya , Lu Jing , Niyazi Mayinuer 
Published: July 2020

Abstract
Background: PGF and TNFAIP2 are important angiogenic factors, which were abnormal expression in cervical cancer (CC). However, no report was investigating the associations between the polymorphisms in the PGF and TNFAIP2 genes and CC risk. Methods: We conducted a case-control study of 342 CC patients and 498 cancer-free controls in Chinese Uygur female population. Three selected SNPs (PGF rs8019391, PGF rs2268615, and TNFAIP2 rs710100) were genotyped to investigate the possible association of the polymorphisms in PGF and TNFAIP2 with the risk of CC. The analysis adjusted by age was used to assess associations of these SNPs with CC risk. Results: PGF rs2268615 (OR = 1.39, 95% CI = 1.04-1.86, p = 0.024) and TNFAIP2 rs710100 (OR = 1.44, 95% CI =1.07-1.95, p = 0.018) polymorphisms were significantly associated with the increased risk of CC. Moreover, PGF rs8019391 T allele was highly represented in patients with III–IV tumor stage (OR = 2.17, p = 4.58´10-4). MDR analysis revealed a positive interaction between the SNPs. Conclusion: Our data suggested that PGF rs2268615, and TNFAIP2 rs710100 polymorphisms may be risk factors for susceptibility to CC, which contributed to the increased risk of CC, and this finding requires further validation by larger studies.Trail registration: Not applicable.
A cluster-randomized trial of workplace ergonomics and neck-specific exercise versus ergonomics and health promotion for office workers to manage neck pain—a secondary outcome analysis
Authors: Johnston Venerina , Chen Xiaoqi , Welch Alyssa , Sjøgaard Gisela , Comans Tracy A , McStea Megan , Straker Leon , Melloh Markus , Pereira Michelle , O'Leary Shaun 
Published: July 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Malaria outbreak in Riaba district, Bioko Island, in 2019 – Lessons learnt
Authors: Guerra Carlos A , Fuseini Godwin , Donfack Oliver Tresor , Smith Jordan M , Mifumu Teresa Ayingono Ondo , Akadiri Gninoussa , Eyang Delicias Esono Mba , Eburi Consuelo Oki , Vaz Liberato Motobe , Micha Víctor Mba , Okenve Leonor Ada , Janes Christopher R , Andeme Ramona Mba , Rivas Matilde Riloha , Phiri Wonder P , Slotman Michel A , Smith Dave L , García Guillermo Alberto 
Published: July 2020

Abstract
At the beginning of 2019, a sudden surge of malaria cases was observed in the district of Riaba, Bioko Island. Between January and April, confirmed malaria cases increased 3.8-fold compared to the same period in 2018. Concurrently, anopheline human biting rate (HBR) increased 2.1-fold. During the outbreak, 82.2% of the district population was tested for malaria with a rapid diagnostic test; 37.2% of those tested had a detectable infection and were treated according to national guidelines. Vector control interventions, including indoor residual spraying and larval source management were scaled-up. After the interventions, the number of confirmed cases decreased by 70% and the overall parasite prevalence in the communities by 43.8%. Observed prevalence in a follow up malaria indicator survey, however, was significantly higher than elsewhere on the island and higher than in previous years. There was no significant reduction in HBR, which remained high for the rest of the year. The surge was attributed to various factors, chiefly increased rainfall and a large number of anthropogenic anopheline breeding sites created by construction works. This case study highlights the need for sustained vector control interventions and multi-sector participation, particularly in malaria control and elimination settings with persistently high local malaria receptivity.
Formation of the Rustenburg Layered Suite by assimilation &#x2013; batch crystallization (ABC) and &#x2013; fractional crystallization (AFC)
Authors: Yao Zhuosen , Mungall James , Jenkins M 
Published: July 2020

Abstract
The Rustenburg Layered Suite (RLS) of the Bushveld Complex of South Africa is a vast layered accumulation of mafic and ultramafic rocks. The layers are widely assumed to result from fractional crystallization from a melt-dominated magma chamber. We derive compositions of all units of the RLS with thermodynamic models of assimilation of crust by a komatiitic parent magma. Ultramafic U-type cumulate layers represent crystal mush produced by single stages of assimilation-batch crystallization (ABC). Anorthositic (A-type) magma mushes emerged by a second stage of batch crystallization during ascent of melts supernatant to mid-crustal U-type cumulates. Only the ferrobasaltic magma of the Upper and Upper Main Zone formed via classical assimilation-fractional crystallization (AFC) and ponded in a melt-dominated magma chamber that subsequently evolved by fractional crystallization. Mineral deposits associated with reversals between mafic and ultramafic layers, hitherto attributed to magma chamber processes, might form in small intrusions entirely lacking melt-dominated magma chambers.