bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Magnitude and Determinants of Postnatal Care Service Utilization in Farta District, South Gondar Zone, Amhara Region, Ethiopia.
Authors: Mengistu Wondimu Girma , Birhanu Abrham Yeneneh , Demissie Tadesse Wuletaw , Desalgn Abaynew Honelgn 
Published: December 2020

Abstract
BackgroundThe low access to emergency obstetric care services in rural Ethiopia showed that maternal mortality was unlikely to significantly decline. Despite the prior attention given to MCH service to the rural communities, the achievements so far were not satisfactory.MethodsCross-sectional study was carried out among 737mothers in 11Keble’s and a multi-stage sampling technique was used to select the subjects and to assess the magnitude and determinants of postnatal care service utilization in Farta district, South Gondar Administrative Zone, Amhara Region, Ethiopia, in 2019.Binary logistic regression statistical model using the adjusted odds ratio (AOR) and 95% CI was used to identify determinants of PNC services utilization.ResultsThe magnitude of PNC services utilization in Farta District was 26.19 % [95% CI: (22.8-29.2)] with variations in between Kebeles. Having formal education of mother [95 % CI: 2.8(1.75-4.48)], birth at a health facility [95% CI: 3.18 (1.73-5.85)], counseling given during ANC visits [(95 %CI: 5.72(3.42-9.55)], complications during or after delivery [95%CI: 2.78(1.20-6.52)] and living within 5km of health facility [95 % CI: 2(1.29-3.10] were identified as determinants of PNC services utilization.ConclusionHaving formal education, counseling during ANC and facility delivery were positively associated whereas having no complications during or after delivery and residing far from a health facility were negatively associated with PNC services utilization. Tracing mechanisms for reaching pregnant women should be developed and strengthened to improve counseling services at home. Frequent support and supervision for remote Kebeles.
The Long Non-coding RNA LINC01133 Promotes Hepatocellular Carcinoma Progression by Sponging miR-199a-5p and Activating Annexin A2
Authors: Yin Dan , Hu Zhi-Qiang , Luo Chu-Bin , Wang Xiao-Yi , Xin Hao-Yang , Sun Rong-Qi , Wang Peng-Cheng , Fan Jia , Zhou Jian , Zhou Zheng-Jun , Zhou Shaolai 
Published: December 2020

Abstract
Background: Long non-coding RNAs (lncRNAs) have been found to be functionally associated with cancer development and progression. Although copy number variations (CNVs) are common in hepatocellular carcinoma (HCC), little is known about how CNVs in lncRNAs affect HCC progression and recurrence.Methods: We analyzed the whole genome sequencing (WGS) data of matched cancerous and non-cancerous liver samples from 49 patients with HCC to identify lncRNAs with CNVs. The results were validated in another cohort of 238 paired HCC and non-tumor samples by TaqMan copy number assay. Kaplan-Meier analysis and the log-rank test were performed to determine the prognostic value of CNVs in lincRNAs. Loss- and gain-of-function studies were conducted to determine the biological functions of LINC01133 in vitro and in vivo. The competing endogenous RNAs (ceRNAs) mechanism was clarified by microRNA sequencing (miR-seq), quantitative real-time PCR (qRT-PCR), western blot, and dual-luciferase reporter analyses. The protein binding mechanism was confirmed by RNA pull-down, RNA immunoprecipitation (RIP), qRT-PCR, and western blot analyses.Results: Genomic copy number of LINC01133 was increased in HCC, which is positively related with the elevated expression of LINC01133. Increased copy number of LINC01133 predicted the poor prognosis in HCC patients. LINC01133 overexpression promoted proliferation, colony formation, migration, and invasion in vitro, and facilitated tumor growth and lung metastasis in vivo, whereas LINC01133 knockdown had the opposite effects. Mechanistically, LINC01133 acted as a sponge of miR-199a-5p, resulting in enhanced expression of SNAI1, which induced epithelial-mesenchymal transition (EMT) in HCC cells. In addition, LINC01133 interacted with Annexin A2 (ANXA2) to activate ANXA2/STAT3 signaling pathway.Conclusions: LINC01133 promotes HCC progression by sponging miR-199a-5p and interacting with ANXA2. LINC01133 CNV gain is predictive of poor prognosis in HCC patients undergoing curative resection.
Multi-Project Model Program Runs With Algorithms to Improve Indian Coal Mining
Authors: Samanta Binay Kumar , Ramesh Dharavath 
Published: December 2020

Abstract
Mining engineering is the most dangerous peace-time profession in the world. Primary Organization for coal mining in India is Coal India and extensive research has been conducted to improve performance. Development computerized methods emphasize better project scheduling, and converting scheduling and monitoring of a project in common package format for the multi-projects of a company The authors have also developed models for cost benefit analysis of improved systems of mining operations on ground realities and database models. The authors have developed for viable mining data-based computer methods in 14 original models, of which few are detailed for lack of space. India has 320 billion tonnes of coal reserve and it is 7 per cent of the reserve of the world. The energy consumption in India is about one-third of the world average and 60 per cent of installed capacity of energy in the country is based on thermal plants.
The Spatiotemporal Distribution of Historical Malaria Cases in Sweden: A Climatic Perspective
Authors: Chen Tzu Tung , Ljungqvist Fredrik Charpentier , Castenbrandt Helene , Hildebrandt Franziska , Ingholt Mathias Mølbak , Hesson Jenny C. , Ankarklev Johan , Seftigen Kristina , Linderholm Hans W. 
Published: December 2020

Abstract
Background: Understanding of the impacts of climatic variability and change on human health, and the spread of diseases, remains poor despite an increasing burden of vector-borne diseases under global warming. Many confounding social variables make such studies challenging during the modern period while studies of climate-disease relationships in historical times are constrained by a lack of long-term data sets. Previous studies of malaria in historical times have revealed an association with climate in northern Europe. Yet, malaria in Sweden in relation to climate variables is understudied and relationships have never been rigorously statistically established. This study seeks to examine the relationship between malaria and climate fluctuations using several data sources, and to characterise the spatio-temporal variations at parish level during severe malaria years in Sweden between 1749 and 1859. Methods: Pearson (rp) and Spearman's rank (rs) correlation analyses were conducted to evaluate inter-annual relationship between malaria deaths, temperature and precipitation. The climate response to larger malaria events was further explored by Superposed Epoch Analysis, and through Geographic Information Systems analysis to map spatial variations of malaria deaths. Results: The number of malaria deaths showed the most significant positive relationship with warm-season temperature of the preceding year, but less significant against precipitation. The strongest correlation was found between malaria deaths and the mean temperature of the preceding June-August (rs=0.57, p<0.01) during the 1756-1820 period. Most malaria hot-spots, during severe malaria years, concentrated in areas around big inland lakes and southern-most Sweden.Conclusions: Increases in malaria transmission, and hence malaria deaths, in Sweden was linked to high summer temperature during the preceding year. This relationship can be established with statistical confidence from parish records in tandem with the long meteorological series. Our results indicate that unusually warm and/or dry summers may have contributed to malaria epidemics, but with non-linear characteristics, highlighting the difficulties in modeling climate-malaria associations. The inter-annual spatial variation of malaria hot-spots further shows that malaria outbreaks were more pronounced in the southern-most region of Sweden in the first half of the nineteenth century compared to the second half of the eighteenth century.
Intracellular complement C3/C3aR/STAT3 pathway inactivation attenuates retinal degeneration in mice
Authors: Wang Shaojun , Du Lu , Yuan Shunzong , Peng Guang-Hua 
Published: December 2020

Abstract
Background

Age-related macular degeneration (AMD) threatens vision in elderly people globally. Systemic complement C3 has been shown to be tightly associated with AMD. However, the mechanism of how local retinal complement C3 and downstream pathway mediated retinal degeneration is not entirely clear.
Methods

Wild-type, C3 knockout mice and mice treated with a C3aR inhibitor (SB290157), STAT3 inhibitor (SH-4-54), were exposed to sodium iodine (NaIO3), respectively. C3 localization in degenerated retina was detected by using immunostaining. Retinal function was assessed by electroretinography, followed by histological analyses to assess RPE and photoreceptor cell degeneration. Retinal inflammation and mitochondrion were investigated through Real-time PCR, ELISA and RNA-Seq.
Results

We reported here that activation of complement was positively associated with retinal degeneration. Strikingly, intracellular C3 was activated in retinal pigment epithelial (RPE) cells, and subsequently promoted the formation of the membrane-attack complex (MAC), which contributed to apoptosis of RPE cells. Intracellular C3 deficiency or pharmalogical inhibition could rescue RPE cells from apoptosis. Moreover, genetic deletion of C3 or inhibition of C3aR/STAT3 results in alleviating immune response and rescue of photoreceptor cells under oxidative stress. Mechanistically, through RNA-Seq, we identified C3/C3aR/STAT3 pathway functionally mediated immune response and photoreceptor cell degeneration.
Conclusions

These results indicate that inhibition of the C3/C3aR/STAT3 pathway might be a new therapeutic intervention for AMD and other retinal degeneration diseases.
Use of Research Evidence Varied in Efforts to Expand Pharmacist Prescriptive Authority: Recommendations to Increase Research Utilization
Authors: Kumar Akshara , Ray Amber Bivins , Blanchard Carrie 
Published: December 2020

Abstract
Background: An expanding body of literature shows that pharmacists’ interventions improve health outcomes and are cost-saving. However, diverse state regulations of pharmacists’ scope of practice create a discrepancy between what pharmacists are trained to do and what they legally can do. This study hopes to investigate stakeholder utilization of research evidence to expand scope of practice in their respective states.Methods: Using autonomous pharmacist prescriptive authority as a surrogate for general pharmacist scope of practice, a general policy document analysis was performed to understand the scope of practice landscape for pharmacists across the United States. Next, a systemized review and semi-structured interviews were conducted to explore how the identified states in the policy document analysis utilized evidence during the policy make process. Investigators analyzed findings from the systemize review and transcribed interviews through application of the SPIRIT Action Framework. Resulting codes were summarized across themes, and recommendations to researchers about increasing utilization of research evidence were crafted. Results: Sixteen states with 27 autonomous pharmacist prescriptive authority policies were identified. The systemized review yielded no relevant peer-review literature regarding evidence utilization, so gray literature and interviews were analyzed. Public health need and safety considerations motivated evidence engagement, while key considerations dictating utilization of research included perceptions of research, access to resources and experts, and the successful implementation of similar policy. Research evidence helped to advocate for and set terms for pharmacist prescribing. Barriers to research utilization include stakeholder opposition to pharmacist prescribing, inability to interpret research, and a lack of relevant evidence. Recommendations for researchers include investigating specific metrics to evaluate scope of practice policy, developing relationships between policymakers and researchers, and leveraging pharmacy practice stakeholders.Conclusions: Overall, alignment of researcher goals and legislative priorities, coupled with timely communication, may help to increase research evidence engagement in pharmacist scope of practice policy. By addressing these factors regarding research engagement identified in this study, researchers can increase evidence-based scope of practice, which can help to improve patient outcomes, contain costs, and provide pharmacists with the legal infrastructure to practice at the top of their license.
TGF-β gene polimorphisms as risk factors for asthma control among clinic patients.
Authors: Panek Michał , Stawiski Konrad , Kuna Piotr 
Published: December 2020

Abstract
Background: TGF-β and its receptors play a key role in asthma pathogenesis, bronchial hyperreactivity and bronchial remodeling. Expression of isoforms 1–3 of TGFβ cytokine is influenced by tagging polymorphisms in the TGFβ1, TGFβ2 and TGFβ3 gene, and these SNPs may be associated with the risk of asthma development and severity as well as with other diseases. Polymorphic forms of TGF-β1, TGF-β2 and TGF-β3 genes regulate the degree of bronchial inflammation, deterioration of lung functional parameters in spirometry and elevated level of total IgE. All this results in intensification of disease symptoms. According to current GINA 2020 guidelines, the Asthma Control Test (ACT™) should be applied to assess asthma symptoms.Methods: An analysis of polymorphisms localized in TGF-β1, TGF-β2 and TGF-β3 genes was conducted on 652 DNA samples with an application of the MassARRAY® system using the mass spectrometry technique MALDI TOF MS. The degree of asthma control was evaluated with ACT™.Results: The occurrence of the T / C genotype in rs8109627 (p = 0.0171) in the TGF-β1 gene is significantly associated with a higher ACT result (controlled asthma) in a multivariate linear regression analysis model after using backward stepwise selection of variables. In addition, in the linear model for prediction of ACT score we showed SNP rs8109627 (p = 0.0497) in the TGF-β1 gene (improvement of the disease control - controlled asthma) and rs2796822 (p = 0.0454) in the TGF-β2 gene (deterioration of the diseases control - uncontrolled asthma) significantly modify the degree of asthma control.Discussion: We described clinical significance of two SNPs in two genes TGF-β1 and TGF-β2, as yet unknown. We proved that the use of both genotypes and MAC allows to create a moderately correct prognostic model which is about 70% efficient on the entire set of analyzed SNPs in TGF-β1, TGF-β2, and TGF-β3 genes.
The Role of Hyperleukocytosis in Predicting the Severity of Pertussis
Authors: Wu Xiao-Ying , gan chuan 
Published: December 2020

Abstract
Background: Few reports have described the relationship between WBC count and the severity of pertussis or the timing of ET in patients with hyperleukocytosis.Methods: A retrospective clinical analysis of infants with pertussis and a WBC exceeding 30*10^9/L was performed.Results: A total of 158 patients were enrolled in the study. There were significant differences in the clinical manifestations of cyanosis, fever, highest respiratory rate, and highest heart rate. There were significant differences in all complications except for pulmonary hypertension. In laboratory findings, there were significant differences in organ damage (myocardial markers, ALT), increased inflammation indicators (CRP, PCT), and the incidence of combined bacterial infections. There were significant differences in ICU stay length, mechanical ventilation use, days hospitalized, days until cough relief and days until the WBC fell below 25*10^9/L. A WBC count >55.38 *10^9/L was calculated as the cutoff value with 88.2% sensitivity and 23.4% specificity in predicting ET. A respiratory rate of 59 breaths/min had 94.1% sensitivity and 36.7% specificity in predicting ET. A heart rate of 159 beats/min had 100% sensitivity and 38.1% specificity in predicting ET.Conclusion: WBC count is related to the severity of pertussis. We recommend that ET is considered when the patient’s WBC count exceeds 55*10^9/L, breathing exceeds 60 breaths/min, and/or heart rate exceeds 160 beats/min.
Is COVID-19 a Disease of Poverty: The Relationship Between Food Insecurity and the Likelihood of Hospitalization in Patients with COVID-19
Authors: ariya mohammad , Karimi Jalal , Abolghasemi Somayeh , Hematdar Zeinab , Naghizadeh Mohammad Mehdi , Moradi Maryam , Barati-Boldaji Reza 
Published: December 2020

Abstract
Background: The World Health Organization (WHO) has declared the Corona pandemic as a public health emergency. This pandemic affects the main pillars of food security. This study aimed to investigate the relationship between food insecurity and the probability of hospitalization and the length of the recovery period after getting COVID-19.Methods: This cross-sectional study was performed through the census on COVID-19 patients diagnosed in Fasa, Iran. Informed consent, demographic, and food security questionnaire were completed over the phone. Then, all patients were followed up until recovery. Data were analyzed using SPSS26 and Chi-square test, t-test, and logistic regression (P> 0.05).Results: In this study, 219 COVID-19 patients [100 (54.7%) male and 119 female (54.3%)] with a mean age of 40.05±15.54 years old were examined. Possibility of hospitalization and the length of the recovery period of more than one month was significantly longer in the food insecure group (P = 0.001) and (P = 0.76), respectively, but the mean length of hospital stay in the two groups was not significantly different (P = 0.76). After adjusting for all confounding variables, people with food insecurity were 3.9 times more likely to be hospitalized than those with food security. Conclusions: We observed that food-insecure people were significantly more likely to be hospitalized than the secure group.
Comparison of a Fast Track Protocol and Standard Care After Hip Arthroplasty in the Reduction of the Length of Stay and The Early Weight-Bearing Resumption: Study Protocol for a Randomized Controlled Trial
Authors: Rocchi Martina , Stagni Cesare , Govoni Marco , Mazzotta Alessandro , Vivarelli Leonardo , Magli Antonella Orlandi , Perrone Mariada , Benedetti Maria Grazia , Dallari Dante 
Published: December 2020

Abstract
BackgroundTo date, the hip arthroplasty is one of the most commonly performed surgical procedures, with a growing worldwide demand. In recent decades, major progress made in terms of surgical technique, biomechanics and tribology knowledge have contributed to improve the medical and functional management of the patient. This study aims to assess if the application of a fast track protocol, consisting of a pre-operative educational intervention, adequate post-operative pain control and intensive rehabilitation intervention, reduces length of stay (LOS) and allows the early functional recovery compared to normal clinical practice for patients undergoing hip arthroplasty.MethodsThe study population consists of 90 patients with primary arthrosis of the hip with an anterior indication of hip arthroplasty. The exclusion criteria are older than 70 years, a contraindication to performing spinal anaesthesia and bone mass index (BMI) greater than 32. Participants, 45 for each group, are randomly allocated to one of two arms: fast track clinical pathway or standard care protocol. During allocation, baseline parameters such as Harris Hip Score (HHS) and Western Ontario and McMaster Universities index (WOMAC) are collected. On the third postoperative day, the functional autonomy for each patient is assessed by the Iowa Level of Assistance (ILOA) scale and it is expected the discharge for patients in the fast track group (primary outcome). On the other hand, standard care patient discharge is expected after 5-7 days after surgery. During follow-up fixed at 6 weeks, 3, 6 and 12 months, HHS and WOMAC scores are collected for each patient (secondary outcomes).DiscussionAlthough total hip replacement has become a widespread standardized procedure, at the author’s knowledge, only few randomized controlled trial were performed to evaluate the effectiveness of fast track pathway vs. standard care procedure in the reduction of the LOS after hip arthroplasty. It is expected that our results collected by the application of minimally invasive surgical interventions with concomitant management of perioperative pain and bleeding, and early functional rehabilitation will contribute to enrich the understanding of clinical and organizational aspects linked to fast track arthroplasty. Trial registrationClinicalTrials.gov, NCT03875976, registered on 15 March 2019 – ‘Retrospectively registered’, https://clinicaltrials.gov/ct2/show/NCT03875976
Pendampingan Kader Pesantren Sebagai Aset Modal Sosial dalam Penguatan Moderasi Beragama
Authors: Hefni Wildani , Uyun Qurrotul 
Published: December 2020

<p><em>Islamic boarding schools are an important element of education in Indonesia. Islamic boarding school educates students to understand and practice religion with a sense of tolerance and moderate. However, recently, the moderate nature of Indonesian Islam is challenged by the spirit of radicalism and conservatism Islam. In this context, this community service on mainstreaming religious moderation among students at Darus Sholah Islamic boarding school is very relevant to spread moderation and tolerant values in religious understanding. Strengthening religious moderatism among students at an Islamic boarding school is one of the strategies to prevent radicalism. Santri as a part of Islamic boarding schools plays an important role to build a social life in the process of socialization and internalization of socio-cultural values about harmony and peace as social capital. The result of this community service on strengthening religious moderatism among students of Islamic boarding schools will make the community of students at Islamic boarding school more aware and contribute to conveying religious understanding following the Islamic boarding school traditions.<strong></strong></em></p><p><strong><em> </em></strong></p><p>Pondok pesantren adalah satu elemen penting dalam dunia pendidikan di Indonesia. Pondok pesantren telah lama mengajarkan ilmu agama dengan nilai-nilai yang penuh toleransi dan moderat. Namun akhir-akhir ini, moderatisme Islam menghadapi tantangan dengan lahirnya pemahaman keislaman yang radikal dan konservatif. Dalam konteks ini, program pendampingan dan pemberdayaan kader pesantren di Darus Sholah menemukan relevansinya dengan tujuan untuk menguatkan serta menyebarluaskan pemahaman yang moderat dan toleran. Penguatan moderasi beragama dikalangan para santri merupakan salah satu strategi untuk mencegah lahirnya radikalisme. Santri sebagai bagian tak terpisahkan dari dunia pesantren memiliki peran yang cukup signifikan untuk menumbuhkan kehidupan sosial yang harmoni dan penuh dengan nilai perdamaian. Hasil dari pendampingan ini diharapkan dapat memberikan manfaat khususnya kepada para kader pesantren untuk lebih sigap dalam membumikan nilai-nilai pesantren yang moderat dan toleran. <em></em></p>