bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knowledge about Diabetic Retinopathy, Eye Check-up Practice and Associated Factors among Adult Patients with Diabetes Mellitus Attending at Debark Hospital, Northwest Ethiopia
Authors: Assem Abel Sinshaw , Tegegne Mebratu Muusew , Alemu Destaye Shiferaw , Woredekal Asamere Tsegaw , Tefera Tsehay Kassa 
Published: November 2020

Abstract
Background: Routine eye examination plays a vital role in detecting diabetic retinopathy in its earliest stage before the onset of blindness. Patients’ knowledge about the nature and the consequences of diabetic retinopathy and routine eye checkup helps for timely identification and early treatment. However, there is limited evidence on knowledge of patients with diabetes mellitus on diabetic retinopathy and their eye check-up practices in Ethiopia. The aim of this study was to assess knowledge about diabetic retinopathy, eye check-up practice and associated factors of diabetic retinopathy among adult diabetic patients at Debark hospital, Northwest Ethiopia. Methods: Institution based cross-sectional study was conducted at Debark hospital, Northwest Ethiopia, from April 20/2018- May 20/2018. A pretested interviewer administered structured questionnaire was used to collect data among 230 diabetic patients aged 18 years and above. Data were entered in to Epi Info version 7 and exported to SPSS version 20 for analysis. Bivariable and multivariable binary logistic regression analyses were done. Odds ratio with 95% confidence level was determined and variables with p–value of < 0.05 were considered as statistically significant. Result: Out of 238 sample 230 were participated, among this, 119 (51.7%) were males. The mean age of the respondents was 49 (SD ±17.6) years. One hundred nine (47.4%) participants had good knowledge and 91 (39.6%) had good eye check-up practice. Urban residence [AOR= 2.65;95% CI: 1.16-6.07)]), monthly income of 3501-8000 birr [AOR=4.54;(1.31-15.7)], type II diabetes mellitus [AOR=3.9;(1.6-9.6)], duration of diabetes (6-12 years[AOR=4.4;(1.4-13.5)]), history of eye disease [AOR=5.5;(2.3-13.0)] were associated with good knowledge. Similarly, longer duration of diabetes (13-25 years [AOR=3.77; (1.05-13.5)]) and history of eye disease [AOR=2.47; (1.09-5.62)] were associated with good eye check-up practice. Conclusion: The proportion of good knowledge about diabetic retinopathy among diabetic patients at Debark hospital was fair (47.4%) and good eye check-up practice (eye examination at least once in the past year) was low (39.6%). Longer duration of diabetes and history of eye disease were identified as positive factors for good knowledge and eye check-up practice. Knowledge and regular eye check-up practice needs to be enhanced through provision of appropriate health education.
Increased Association of Deamidated αA-N101D with Lens Membrane of Transgenic αAN101D vs. Wild Type αA Mice: Potential Effects on Intracellular Ionic Imbalance and Membrane Disorganization&nbsp;
Authors: Srivast Om , Srivast Kiran , Joseph Roy , Wilson Landon 
Published: November 2020

Abstract
We have generated two mouse models, in one by inserting the human lens αAN101D transgene in CRYαAN101D mice, and in the other by inserting human wild-type αA-transgene in CRYαAWT mice. The CRYαAN101D mice developed cortical cataract at about 7-months of age relative to CRYαAWT mice. The objective of the study was to determine the following relative changes in the lenses of CRYαAN101D- vs. CRYαAWT mice: age-related changes with specific emphasis on protein insolubilization, relative membrane-association of αAN101D vs. WTαA proteins, and changes in intracellular ionic imbalance and membrane organization. Methods: Lenses of varying ages from CRYαAWT and CRYαAN101D mice were compared for an age-related protein insolubilization. The relative lens membrane-association of the αAN101D- and WTαA proteins in the two types of mice was determined by immunohistochemical-, immunogold-labeling-, and western blot analyses. The relative levels of membrane-binding of recombinant αAN101D- and WTαA proteins was determined by an in vitro assay, and the levels of intracellular Ca2+ uptake and Na, K-ATPase mRNA were determined in the cultured epithelial cells from lenses of the two types of mice.Results: Compared to the lenses of CRYαAWT, the lenses of CRYαAN101D mice exhibited: (A) An increase in age-related protein insolubilization beginning at about 4-months of age. (B) A greater lens membrane-association of αAN101D- relative to WTαA protein during immunogold-labeling- and western blot analyses, including relatively a greater membrane swelling in the CRYαAN101D lenses. (C) During in vitro assay, the greater levels of binding αAN101D- relative to WTαA protein to membranes was observed. (D) The 75% lower level of Na, K-ATPase mRNA but 1.5X greater Ca2+ uptake were observed in cultured lens epithelial cells of CRYαAN101D- than those of CRYαAWT mice. Conclusions: The results show that an increased lens membrane association of αAN101D--relative WTαA protein in CRYαAN101D mice than CRYαAWT mice occurs, which causes intracellular ionic imbalance, and in turn, membrane swelling that potentially leads to cortical opacity.
Oxytocin Use in Trial of Labor after Cesarean and Its Relationship with Risk of Uterine Rupture in Women with One Previous Cesarean Section: A Meta-analysis of Observational Studies
Authors: ZHANG Huan , LIU Haiyan , LUO Shouling , Gu Weirong 
Published: November 2020

Abstract
Background: Trial of labor after a previous cesarean delivery (TOLAC) has reduced the rate of cesarean sections (CS). Nevertheless, the widespread use of TOLAC has been limited by an increase in adverse outcomes, the most serious one being the risk of symptomatic uterine rupture, which is possibly associated with oxytocin. In this meta-analysis, we explored the risk association between oxytocin use and uterine rupture in TOLAC.Methods: Multiple electronic databases (PubMed, Embase, Web of Science, and Google Scholar) were searched for cross-sectional studies reporting on TOLAC, oxytocin and uterine rupture, which were published between January 1986 and October 2019. The bias-corrected Hedge's g was calculated as the effect size using the random-effects model. A two-sample Z test was used to compare the differences in synthetic rates between groups. The Newcastle-Ottawa Scale (NOS) was used to evaluate the risk of bias. Quality of the evidence was assessed with the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) certainty ratings system.Results: A total of 14 studies, which included 48457 women who underwent TOLAC, met the inclusion criteria. The pooled rate of vaginal birth after a cesarean section (VBAC) and the rate of uterine rupture in spontaneous labor were 74.3% and 0.7%, respectively. In addition, the pooled rate of VBAC and the rate of uterine rupture in the induction labor group was 60.7% and 2.2%, respectively. The women who had spontaneous labor had a significantly higher rate of VBAC (p=0.0032) and a lower rate of uterine rupture (p=0.0003) compared to induced labor. The pooled rates of uterine rupture in women using oxytocin and women not using oxytocin in TOLAC were 1.4% and 0.5%, respectively, and the difference was significant (p=0.0002). Also, the synthetic rate of uterine rupture in oxytocin augmentation among women with spontaneous labor and women who had a successful induction of labor were 1.7% and 2.2%, respectively, without significant difference (p=0.443).Conclusions: Women with induced labor had a higher risk of uterine rupture compared to women with spontaneous labor following TOLAC. Oxytocin use may increase this risk, which could be influenced by the process of induction or individual cervix condition. Consequently, simplified and standardized intrapartum management, precise protocol, and cautious monitoring of oxytocin use in TOLAC are necessary.
Fabrication of Nanomechanical Biosensors to Map 3D Surface Strain in Live Cells and Tissue
Authors: Shiwarski Daniel J. , Tashman Joshua W. , Tsamis Alkiviadis , Bliley Jacqueline M. , Blundon Malachi A. , Aranda-Michel Edgar , Jallerat Quentin , Szymanski John M. , McCartney Brooke M. , Feinberg Adam W. 
Published: November 2020

Abstract
This protocol details the step-by-step fabrication process to create an extracellular matrix (ECM) protein-based nanomechanical biosensor (NMBS) via an adaptation of the surface-initiated assembly (SIA) procedure1. The NMBS is a fluorescently labeled, ultra-thin fibronectin lattice-mesh with spatial resolution tailored by adjusting the width and spacing of the lattice from 2-100 µm. By applying the NMBS to the surface of cells and tissues one can directly measure deformation from subcellular to tissue length-scales. The procedure outlined here covers all aspects of NMBS construction from pattern design, photolithography for mold creation, casting of polydimethylsiloxane (PDMS) silicone stamps, conjugation of fluorescent dye to fibronectin, microcontact printing of fibronectin, creation of gelatin carriers2, transfer of the NMBS to gelatin carriers, and the use of gelatin carriers for application of the NMBS onto cells and tissue. The protocol can be broken down into three phases: material preparation (2 days), NMBS patterning (4 hours), and transfer to cell or tissue (1 hour). Material preparation and NMBS patterning can be performed ahead of time and the patterned NMBS can be stored for up to 1 year prior to use.
Grain-boundary-rich 2D materials for attomolar-ability biochemical sensors
Authors: Xue Tianyu , Liu Lixuan , Ye Kun , Lin Changqing , Jia Zhiyan , Nie Anmin , Cheng Yingchun , Xiang Jianyong , Mu Congpu , Wang Bochong , Wen Fusheng , Zhai Kun , Zhao Zhisheng , Gong Yongji , Liu Zhongyuan , Tian Yongjun 
Published: November 2020

Abstract
Next-generation high-performance biological and chemical sensors based on the emerging multitudinous two-dimensional (2D) layered materials have been attracting great attention in recent years. The performance of 2D biochemical sensors is strongly dependent on the structural defects, which provide indispensable active sites for sensitive and selective adsorption of analytes. However, achieving controllable defect engineering is still a big challenge. In the present work, we propose achieving superior biochemical sensor performance with high-surface-density grain boundaries (GBs), a kind of ubiquitous structural defects, in polycrystalline 2D thin films, which can be controllably synthesized. As a proof-of-concept, by utilizing the high-density GBs in monolayer (1L) WS2 films, we fabricated a series of surface plasmon resonance (SPR) sensors for mercury ion (Hg2+) detection. Our investigation has demonstrated substantial sensitivity enhancement of Hg2+ detection down to trace attomolar-level quantification (detection limit of 1 aM), which is ascribed to the abundance of active sites on high-density GBs. This work provides a promising avenue for the design of ultra-sensitive sensors toward commercialized products based on the GB-rich 2D layered materials.
Real-time imaging of accelerated solid-liquid-gas reactions with nanobubbles
Authors: Wang Wen , Xu Tao , Chen Jige , Shangguan Junyi , Dong Hui , Ma Huishu , Zhang Qiubo , Bai Tingting , Guo Zhirui , Fang Haiping , Zheng Haimei , Sun Litao 
Published: November 2020

Abstract
Solid-liquid-gas reactions are ubiquitous. An understanding of how gases influence the reactions at the nanoscale is significant for achieving the enhanced triple-phase reactions. Here, we report a real-time observation of the accelerated etching of gold nanorods with oxygen nanobubbles in aqueous hydrobromic acid using liquid cell transmission electron microscopy (TEM). Our observation reveals that when an oxygen nanobubble is close to a nanorod below the critical distance (~1nm), the local etching rate is significantly enhanced with over an order of magnitude faster. Molecular dynamics simulations results show that the strong attractive van der Waals interaction between the gold nanorod and oxygen molecules facilitates the transport of oxygen through the thin liquid layer to the gold surface and thus plays a crucial role in increasing the etching rate. This result sheds light on the rational design of solid-liquid-gas reactions for enhanced activities.
Quantum many-body attractors
Authors: Buca Berislav , Purkayastha Archak , Guarnieri Giacomo , Mitchison Mark , Jaksch Dieter , Goold John 
Published: November 2020

Abstract
Real-world complex systems often show robust, persistent oscillatory dynamics, e.g.~non-trivial attractors. On the quantum level this behaviour has only been found in semi-classical or weakly correlated systems under restrictive assumptions. However, strongly interacting systems without classical limits, e.g.~electrons on a lattice or spins, typically relax quickly to a stationary state (trivial attractors). This raises the puzzling question of how non-trivial attractors can arise from the quantum laws. Here, we introduce strictly local dynamical symmetries that lead to extremely robust and persistent oscillations in quantum many-body systems without a classical limit. Observables that do not have overlap with the symmetry operators can relax, losing memory of their initial conditions. The remaining observables enter complex dynamical cycles, signalling the emergence of a quantum many-body attractor. We provide a recipe for constructing Hamiltonians featuring local dynamical symmetries. As an example, we introduce the spin lace – a model of a quasi-1D quantum magnet.
Single-cell Transcriptome of Bronchoalveolar Lavage Fluid Reveals Dynamic Change of Macrophages During SARS-CoV-2 Infection in Ferrets
Authors: Lee Jeong Seok , Koh June-Young , Yi Kijong , Kim Young-Il , Park Su-Jin , Kim Eun-Ha , Kim Se-Mi , Park Sung Ho , Ju Young Seok , Choi Young Ki , Park Su-Hyung 
Published: November 2020

Abstract
Although the profile of immune cells changes during the natural course of SARS-CoV-2 inflection in human patients, few studies have used a longitudinal approach to reveal their dynamic features. Here, we performed single-cell RNA sequencing of bronchoalveolar lavage fluid cells longitudinally obtained from SARS-CoV-2-infected ferrets. Landscape analysis of the lung immune microenvironment showed dynamic changes in cell proportions and characteristics in uninfected control, at 2 days post-infection (dpi) (early stage of SARS-CoV-2 infection with peak viral titer), and 5 dpi (resolution phase). NK cells and CD8+ T cells exhibited activated subclusters with interferon-stimulated features, which were peaked at 2 dpi. Intriguingly, macrophages were classified into 10 distinct subpopulations, and their relative proportions changed over the time. We observed prominent transcriptome changes among monocyte-derived infiltrating macrophages and differentiated M1/M2 macrophages, especially at 2 dpi. Moreover, trajectory analysis revealed gene expression changes from monocyte-derived infiltrating macrophages toward M1 or M2 macrophages and identified the distinct macrophage subpopulation that had rapidly undergone SARS-CoV-2-mediated activation of inflammatory responses. Finally, we found that different spectrums of M1 or M2 macrophages showed distinct patterns of gene modules downregulated by immune-modulatory drugs. Overall, these results elucidate fundamental aspects of the immune response dynamics provoked by SARS-CoV-2 infection.
Integrated Transcriptome and Proteome Analysis Reveals Complex Regulatory Mechanism of Cotton in Response to Salt Stress
Authors: Chen Lin , Sun Heng , Kong Jie , Xu Haijiang , Yang Xiyan 
Published: November 2020

Abstract
BackgroundSoil salt stress seriously restricts the yield and quality of cotton worldwide. To investigate the molecular mechanism of cotton response to salt stress, a main cultivated variety Gossypium hirsutum L. acc. Xinluzhong 54 was used to perform transcriptome and proteome integrated analysis. ResultsThrough transcriptome analysis of cotton treated with salt stress for 0 h (T0), 3 h (T3) and 12 h (T12), we identified 8,436, 11,628 and 6,311 differentially expressed genes (DEGs) inT3 / T0, T12 / T0 and T12 / T3, respectively. A total of 459 differentially expressed proteins (DEPs) were identified by proteomic analysis, of which 273, 99 and 260 DEPs were identified in T3 / T0, T12 / T0 and T12 / T3, respectively. Metabolic pathways, biosynthesis of secondary metabolites, photosynthesis and plant hormone signal transduction were the main enrichment pathways by annotation of DEGs or DEPs. Detail analysis of the DEGs or DEPs revealed that complex signal pathways, such as ABA and JA signal, calcium signal, MAPK signal cascade, transcription factors, followed by activation of antioxidant and ion transporters, were identified to participate in regulating salt response in cotton.ConclusionsOur results not only contribute to understand the mechanism of cotton response to salt stress, but also provide nine candidate genes, which might be used for molecular breeding to improve salt-tolerance in cotton.
Genome-Wide Identification and Expression Profile Analysis of WRKY Family Genes in Teak (Tectona Grandis)
Authors: Wang Xi-Yang , Song Jie , Xing Jia-Hui , Liang Jun-Feng , Ke Bi-ying 
Published: November 2020

Abstract
Background: WRKY proteins comprise a large family of transcription factors that play vital roles in many aspects of physiological processes and adaption to environment. However, little information was available about the WRKY genes in teak (Tectona grandis). The recent release of the whole-genome sequence of teak allowed us to perform a genome-wide investigation into the organization and expression profiling of teak WRKY genes. Results: In the present study, 102 teak WRKY (TgWRKY) genes were identified and renamed as per their positions on chromosome and scaffolds. According to their structural and phylogenetic analysis, the 102 TgWRKYs were further classified into three main groups with seceral subgroups. The segmental duplication event played a major role in the expansion of teak WRKY gene family and three WGD events were inferred. Expression profiles derived from transcriptome data exhibited distinct expression patterns of TgWRKY genes in various tissues and inresponse to different abiotic stress.Conclusions: 102 TgWRKY genes were identified in teak and the structure of their encoded proteins, their evolutionary characteristics and expression patterns were examined in this study. This study generated an important resource that will provide helpful information for further exploration of the TgWRKY genes role in the regulatory mechanism in response to abiotic stresses.
Different Pulsed Radiofrequency Modes on&nbsp;Gasserian Ganglion Improved Acute/subacute Zoster-related Trigeminal neuralgia: A Randomized, Double-Blinded, Controlled Trial
Authors: Wan Cheng-fu , Song Tao 
Published: November 2020

Abstract
Background: Trigeminal postherpetic neuralgia (PHN), developed from herpes zoster virus infected on gasserian ganglion, is a severe neuropathic pain and often refractory to existing treatment. Pulsed radiofrequency (PRF) is known to be effective for treating acute/subacute zoster-related pain, but the effectiveness of different PRF mode has not been tested.Objective: This study aimed to investigate the efficacy and safety of different PRF modes on gasserian ganglion in patients with acute/subacute zoster-related trigeminal neuralgia.Methods: 120 patients were equally randomized into 2 groups (n = 60): high-voltage, long-duration PRF(HL-PRF) group and standard PRF(S-PRF) group. One cycle high-voltage, long-duration PRF was applied in HL-PRF group and three cycles standard PRF were performed in S-PRF group. Visual analog scales (VAS), 36-item short form health survey scores (SF-36) and average doses of pregabalin were recorded at different time points. Results: There were significant declined in VAS and SF-36 scores in both two groups at different time point after PRF treatment (P < 0.001). The scores of VAS and SF-36 in HL-PRF group were significantly lower than those in S-PRF group at different time points after treatment (P < 0.05). The dosages of pregabalin were also lower in HL-PRF group at days 3, 14 and 28 after treatment (P < 0.05). There was no serious adverse effect in either group. Conclusion: High-voltage, long-duration PRF on gasserian ganglion is more effective than standard PRF for acute/subacute zoster-related trigeminal neuralgia patients. Clinical Trial Registration: ChiCTR2000038775
Tunable Intermediate-Logic Ternary Circuits based on MoSe2-WSe2 Heterojunction
Authors: Hong Seongin , Park Hyeon Jung , Cho Hae won , Park Junwoo , Kim Sunkook , Yoo Hocheon 
Published: November 2020

Abstract
Van der Waals (vdW) heterojunctions, which consist of p-type and n-type semiconductors, have provided new features for transition metal dichalcogenides (TMDs). In this work, a negative differential transconductance (NDT) transistor based on a MoSe2-WSe2 heterojunction (MoSe2-WSe2 H-TR) is proposed. The MoSe2-WSe2 H-TR provides desirable device characteristics for ternary circuit operation with a switching behavior of off-state / p-type turn-on / NDT region / p-type turn-on. As a result, a 100% output voltage (VOUT) swing inverter can be achieved in a ternary inverter, which consists of the proposed MoSe2-WSe2 vdW-H-TR and a MoS2 floating-gate transistor. Furthermore, a tunable intermediate-logic ternary circuit operation is demonstrated by controlling the threshold voltage (VTH) in a pull-down n-type MoS2 floating-gate transistor. We also investigated that a light-induced operation on the MoSe2-WSe2 vdW-H-TR offers control of the VOUT amplitude at the intermediate-logic state. Based on the proposed MoSe2-WSe2 vdW-H-TR, this work suggests a strategy to obtain a tunable ternary circuit, thus providing a new concept of heterojunction electronics using layered TMDs.
Deep Learning Based BackCom Multiple Beamforming for 6G UAV IoT Networks
Authors: Qi Fei , Li Wenjing , Yu Peng , Feng Lei , Zhou Fanqin 
Published: November 2020

Abstract
Combining unmanned aerial vehicles (UAVs) with 6G, Internet of Things (IoT) and other emerging communication technologies could better satisfy various IoT applications and create more innovative services. This paper develops a novel hierarchical 6G IoT network with UAVs in the sky and intelligent reflective surface (IRS) equipped. The system employs backscattering communication (BackCom) to transmit data in a free-ride manner. Through beamforming, IRS enhances the energy of the reflectable signal, thereby improving the distance and performance of the BackCom. Simulation results reveal that our approach makes a significant improvement to the performance of the whole system, and takes obvious advantage over traditional solutions.