bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enabling evolutionary studies at multiple scales in Apocynaceae through Hyb‐Seq
Authors: Straub Shannon C. K. , Boutte Julien , Fishbein Mark , Livshultz Tatyana 
Published: November 2020

PremiseApocynaceae is the 10th largest flowering plant family and a focus for study of plant–insect interactions, especially as mediated by secondary metabolites. However, it has few genomic resources relative to its size. Target capture sequencing is a powerful approach for genome reduction that facilitates studies requiring data from the nuclear genome in non‐model taxa, such as Apocynaceae.MethodsTranscriptomes were used to design probes for targeted sequencing of putatively single‐copy nuclear genes across Apocynaceae. The sequences obtained were used to assess the success of the probe design, the intrageneric and intraspecific variation in the targeted genes, and the utility of the genes for inferring phylogeny.ResultsFrom 853 candidate nuclear genes, 835 were consistently recovered in single copy and were variable enough for phylogenomics. The inferred gene trees were useful for coalescent‐based species tree analysis, which showed all subfamilies of Apocynaceae as monophyletic, while also resolving relationships among species within the genus Apocynum. Intraspecific comparison of Elytropus chilensis individuals revealed numerous single‐nucleotide polymorphisms with potential for use in population‐level studies.DiscussionCommunity use of this Hyb‐Seq probe set will facilitate and promote progress in the study of Apocynaceae across scales from population genomics to phylogenomics.
Imidazolinium‐based Multiblock Amphiphile as Transmembrane Anion Transporter
Authors: Mori Miki , Sato Kohei , Ekimoto Toru , Okumura Shinichi , Ikeguchi Mitsunori , Tabata Kazuhito V. , Noji Hiroyuki , Kinbara Kazushi 
Published: November 2020

AbstractTransmembrane anion transport is an important biological process in maintaining cellular functions. Thus, synthetic anion transporters are widely developed for their biological applications. Imidazolinium was introduced as anion recognition site to a multiblock amphiphilic structure that consists of octa(ethylene glycol) and aromatic units. Ion transport assay using halide‐sensitive lucigenin and pH‐sensitive 8‐hydroxypyrene‐1,3,6‐trisulfonate (HPTS) revealed that imidazolinium‐based multiblock amphiphile (IMA) transports anions and showed high selectivity for nitrate, which plays crucial roles in many biological events. Temperature‐dependent ion transport assay using 1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine (DPPC) indicated that IMA works as a mobile carrier. 1H NMR titration experiments indicated that the C2 proton of the imidazolinium ring recognizes anions via a (C−H)+⋅⋅⋅X− hydrogen bond. Furthermore, all‐atom molecular dynamics simulations revealed a dynamic feature of IMA within the membranes during ion transportation.
Accelerating HIV‐1 VLP production using stable High Five insect cell pools
Authors: Puente‐Massaguer Eduard , Grau‐Garcia Paula , Strobl Florian , Grabherr Reingard , Striedner Gerald , Lecina Martí , Gòdia Francesc 
Published: November 2020

AbstractStable cell pools are receiving a renewed interest as a potential alternative system to clonal cell lines. The shorter development timelines and the capacity to achieve high product yields make them an interesting approach for recombinant protein production. In this study, stable High Five cell pools are assessed for the production of a simple protein, mCherry, and the more complex HIV‐1 Gag‐eGFP virus‐like particles (VLPs). Random integration coupled to fluorescence‐activated cell sorting (FACS) in suspension conditions is applied to accelerate the stable cell pool generation process and enrich it with high producer cells. This methodology is successfully transferred to a bioreactor for VLP production, resulting in a 2‐fold increase in VLP yields with respect to shake flask cultures. In these conditions, maximum viable cell concentration improves by 1.5‐fold, and by‐product formation is significantly reduced. Remarkably, a global increase in the uptake of amino acids in the Gag‐eGFP stable cell pool is observed when compared with parental High Five cells, reflecting the additional metabolic burden associated with VLP production. These results suggest that stable High Five cell pools are a robust and powerful approach to produce VLPs and other recombinant proteins, and put the basis for future studies aiming to scale up this system.
Trifluridine/tipiracil plus bevacizumab as a first‐line treatment for elderly patients with metastatic colorectal cancer (KSCC1602): A multicenter phase II trial
Authors: Oki Eiji , Makiyama Akitaka , Miyamoto Yuji , Kotaka Masahito , Kawanaka Hirofumi , Miwa Keisuke , Kabashima Akira , Noguchi Tomohiro , Yuge Kotaro , Kashiwada Tomomi , Ando Koji , Shimokawa Mototsugu , Saeki Hiroshi , Akagi Yoshito , Baba Hideo , Maehara Yoshihiko , Mori Masaki 
Published: November 2020

AbstractBackgroundA previous Phase I/II study demonstrated that TAS‐102 (trifluridine/tipiracil [FTD/TPI]) plus bevacizumab (Bev) has encouraging efficacy and controllable safety for patients with previously treated metastatic colorectal cancer. Therefore, we designed for assessing the efficacy and safety of FTD/TPI plus Bev in elderly patients with previously untreated metastatic colorectal cancer.MethodsThis is a multicenter, single‐arm Phase II study included patients ≥70 years old with previously untreated, unresectable metastatic colorectal cancer. Treatment consisted of FTD/TPI plus Bev given every 4 weeks. The primary endpoint was progression‐free survival (PFS), assuming a null hypothesis of a PFS of 5 months. The secondary endpoints were the overall survival (OS), overall response rate (ORR), and adverse events (AEs).ResultsBetween 5 January 2017 and 13 March 2018, 39 patients were enrolled from 18 institutions. The median patient age was 76.0 years (range, 70–88); the ECOG‐PS was 0 in 24 patients and 1 in 15 patients. The median PFS was 9.4 months as a primary endpoint, and the median OS was 22.4 months. The ORR was 40.5% and the disease control rate was 86.5%. Grade 3–4 AEs included neutropenia (71.8%), leukopenia (51.3%), anorexia (15.4%), febrile neutropenia (10.3%), and fatigue (10.3%).ConclusionsFTD/TPI plus Bev is an effective and well‐tolerated regimen for elderly patients with previously untreated metastatic colorectal cancer. Capecitabine/bevacizumab can be selected as a subsequent maintenance therapy without irinotecan and oxaliplatin because FTD/TPI has no cross‐resistance with 5‐fluorouracil.Clinical trial registration: UMIN clinical trials registry (UMIN000025241).
The stage‐specific roles of radiotherapy and chemotherapy in nodular lymphocyte predominant Hodgkin lymphoma patients: a propensity score‐matched analysis of the SEER database
Authors: Wang Shijie , Jia Mingfang , Han Jianglong , Zhang Rui , Huang Kejie , Qiao Yunfeng , Chen Ping , Fu Zhenming 
Published: November 2020

AbstractBackgroundThe stage‐specific roles of radiotherapy (RT) alone, chemotherapy alone, and combined RT and chemotherapy (CRT) for patients with nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) have not been adequately evaluated.MethodsWe analyzed patients with all stages of NLPHL enrolled in the Surveillance, Epidemiology, and End Results (SEER) registry from January 2000 to December 2015. Propensity score (PS) analysis with 1:1 matching (PSM) was performed to ensure the well‐balanced characteristics of the comparison groups. Kaplan–Meier and Cox proportional‐hazards models were used to evaluate the overall survival (OS), cancer‐specific survival (CSS), hazard ratios (HRs), and corresponding 95% confidence intervals (95% CI). Restricted mean survival times (RMST) were also used for the survival analyses.ResultsFor early‐stage patients, CRT was associated with the best survival, the mean OS was significantly improved by approximately 20 months (20 m), and the risk of death was reduced by more than 80%, both before and after PSM (p < 0.05). For advanced‐stage patients, none of RT alone, chemotherapy alone, or CRT had a significant effect on survival. Chemotherapy alone and CRT might be more beneficial for long‐term survival (RMST120 m: neither RT nor chemotherapy vs. chemotherapy alone vs. CRT = 104 m vs. 111 m vs. 108 m). Subgroup analysis of patients with early‐stage NLPHL showed that CRT was associated with better survival of elderly patients (improved OS = 43.8 m, HR = 0.14, p < 0.05). However, the survival benefits of treatments for young patients were not statistically significant. The efficacy of RT was significantly different between the age groups (pfor interaction = 0.020).ConclusionsThese results from SEER data suggest that CRT may be considered for early‐stage NLPHL, especially for elderly patients. Further studies are needed to identify effective treatments in patients with advanced‐stage NLPHL.
Effects of consumer–cause fit and consumer–product fit of cause‐related marketing on product purchase intention
Authors: Sung Hyein , Kim Junyong , Choi Hyewon 
Published: November 2020

AbstractWhile many studies have shown that cause‐related marketing (CRM) is more effective when product‐cause fit (PCF) is high than when it is low, some research has found otherwise. Furthermore, although a few studies demonstrated that high‐PCF CRM is more effective when consumer–cause fit (CCF) or consumer–product fit (CPF) is also high, others have found that the moderating effect of CCF on high‐PCF CRM is insignificant. Given the inevitable co‐occurrence of CCF and CPF in CRM, CPF may moderate the effect of CCF on the effectiveness of high‐PCF CRM. Hence, this research examined the interaction effect between the CCF and CPF on the effect of high‐PCF CRM on consumers' product purchase intention (PPI). The results of an experiment showed that a high‐PCF CRM enhanced the PPI regardless of the CCF when the CPF was low. However, when the CPF was high, high‐PCF CRM enhanced the PPI when the CCF was also high but lowered the PPI when the CCF was low. The findings indicate that, for high‐PCF CRM to affect PPI positively, CCF must be high when CPF is high, whereas high‐PCF CRM may enhance PPI even if CCF is low when CPF is low.
Healing and early stent coverage after ultrathin strut biodegradable polymer‐coated sirolimus‐eluting stent implantation: SiBi optical coherence tomography study
Authors: Abhyankar Atul , Abizaid Alexandre , Chamié Daniel , Patel Gaurang 
Published: November 2020

AbstractAimsThe aim of SiBi study was to evaluate the early vascular healing and neointimal coverage after implantation of ultrathin (60 μm) biodegradable polymer‐coated Tetriflex (Sahajanand Medical Technologies Pvt. Ltd., Surat, India) sirolimus‐eluting stent (SES) using optical coherence tomography (OCT) at 4 to 6 weeks after implantation.MethodsSiBi was a single‐center, observational, investigator‐initiated study. From January 15, 2018 to April 15, 2018, total 29 consecutive patients who had consented and underwent OCT examination at 4–6 weeks after Tetriflex SES implantation were enrolled. All OCT images were analyzed at an independent core laboratory by analysts who were blinded to patient and procedural information.ResultsOf 29 patients, four patients were excluded, as those OCT images were technically inadequate for analysis. Therefore, 25 patients were included in final OCT analysis. Average OCT analysis was performed after 35.3 ± 5 days of Tetriflex implantation. Total 14,024 stent struts in 1,520 cross sections were analyzed. Strut tissue coverage was observed in 91.26 ± 5.53% of struts and malapposed struts were seen in 0.89 ± 1.67%. The mean neointimal hyperplasia (NIH) thickness on the covered struts was 50 ± 30 μm.ConclusionA large percentage of struts were found to be covered with thin layer of NIH evenly distributed along the stent length at around 1 month from stent implantation. The results of this pilot study serve as ethical and scientific backbone to conduct an adequately powered clinical trial to evaluate outcomes of short dual‐antiplatelet therapy in context of ultrathin strut stent.
Impact of yield component alleles from durum wheat on end‐use quality of spring wheat
Authors: Jones Brittney H. , Blake Nancy K. , Heo Hwa‐Young , Kalous Jay R. , Martin John M. , Nash Deanna L. , Torrion Jessica A. , Talbert Luther E. 
Published: November 2020

AbstractBackground and objectivesHard red spring wheat (Triticum aestivum L.) (HRSW) is used for bread baking while durum wheat (Triticum turgidum L. ssp. durum) is used for pasta production. This study investigated the impact of alleles from durum wheat for yield component traits on HRSW end‐use quality traits. Near‐isogenic lines for four quantitative trait loci, QGw.mst‐3B, QGw.mst‐7A, QTn.mst‐5B, and QYld.mst‐2B, were evaluated in rainfed and irrigated environments.FindingsDurum alleles were found to significantly impact end‐use quality at all four loci. The durum allele QGw.mst‐3B for increased kernel weight (KWT) positively impacted end‐use quality traits with increases in kernel protein and loaf volume. The durum allele at QTn.mst‐5B delayed heading date, impacting productive tiller number and KWT and additional end‐use quality traits. The durum allele at QYld.mst‐2B for GY showed both positive and negative effects on end‐use quality. The durum allele for reduced spikelet number per spike at QGw.mst‐7A had little to no impact on end‐use quality.ConclusionsResults from the present study indicate that the alleles from durum wheat are potential sources of cultivar improvement; however, interactions between yield component and end‐use quality traits may influence utilization.Significance and noveltyThis study demonstrates the potential utility of introgressed durum yield component alleles for genetic improvement in HRSW.
Effect of high‐pressure processing on the microbial load and functionality of sugar‐cookie dough
Authors: Sabillón Luis , Stratton Jayne , Rose Devin , Eskridge Kent , Bianchini Andréia 
Published: November 2020

AbstractBackground and objectivesRefrigerated dough products have the potential to be a safety hazard to consumers because they could be consumed raw or undercooked. The objectives of this study were designed to evaluate the microbial and functionality changes in high pressured sugar‐cookie dough as a function of aw (0.80–0.87), pressure level (100–600 MPa), and holding time (1–6 min).FindingsEndogenous microbial populations were marginally reduced (0.2–0.5 log CFU/g) by pressure treatments. However, treating the dough at 600 MPa for 6 min significantly reduced counts of inoculated Escherichia coli by as much as 2.0 log CFU/g. Increasing the aw of cookie dough from 0.80 to 0.87 did not play a significant role in the reduction of microbial counts; however, it yielded a softer and thicker cookie when baked. Dough and cookie physical characteristics did not differ significantly among HPP‐treated and control doughs within the same aw level.ConclusionsThe results of this study suggest that pressure treatment has the potential to improve the microbiological quality of wheat‐based cookie doughs. However, variations in food matrix composition must be considered because some food constituents, such as sugar and fat, may protect microorganisms against pressure‐induced inactivation.Significance and noveltyThe results reported here have practical implications for the food industry and contributes to understand the effects of high‐pressure processing on wheat‐based cookie doughs and their microbial loads.
The first ethanol sclerotherapy of an accessory cavitated uterine mass
Authors: Merviel Philippe , Lelievre Caroline , Cambier Tatiana , Thomas‐Kergastel Isabelle , Dupré Pierre‐François 
Published: November 2020

AbstractAccessory cavitated uterine mass (ACUM) is a very rare, underdiagnosed pathology. It is treated with radical surgery, which results in uterine scarring. Here, we describe the first case of ethanol sclerotherapy of an ACUM, modeled on the treatment of recurring endometrioma. Ethanol sclerotherapy avoids uterine scarring and the secondary risk of uterine rupture and enables the rapid achievement of pregnancy.
A case of early neonate bovine tuberculosis in Ethiopia
Authors: Mekonnen Getnet Abie , Gumi Balako , Berg Stefan , Conlan Andrew J. K. , Ameni Gobena , Wood James L. N. 
Published: November 2020

AbstractThis report illustrates that calves may be infected with bovine tuberculosis at early age under natural conditions and progression can be rapid. Thus, testing of calves needs to be considered in any control program to reduce the risk of transmission.
An unusual and severe case of paclitaxel‐induced hand‐foot syndrome
Authors: Ben Abdallah Ichrak , Ben Nasr Sonia , Zribi Aref , Ayarai Jihene , Fendri Sana , Mehdi Balti , Abderrazek Haddaoui 
Published: November 2020

AbstractAlthough paclitaxel is known to cause mild skin toxicity, it may induce severe HFS requiring drug withdrawal. Patients with high disease burden might receive prolonged paclitaxel chemotherapy. Hence, a grade 2 toxicity would better indicate withdrawal of paclitaxel instead of suspension and rechallenge, to prevent such severe HFS requiring long‐time recovery.
Review on Current Progress of MnO2‐Based Ternary Nanocomposites for Supercapacitor Applications
Authors: Majumdar Dipanwita 
Published: November 2020

AbstractManganese dioxide (MnO2) has proved itself as a popular pseudocapacitive material with low fabrication cost, high availability, low toxicity, and improved handling safety compared to many other inorganics or carbon‐based systems existing in the market. However, the specific capacitance reported to date has been far inferior to that of the theoretically predicted value (ca. 1370 Fg−1), which is mainly attributed to the issues associated with poor conductivity, nanostructure agglomeration, low porosity, rapid electrolyte‐mediated dissolution, and so forth, which have considerably limited its commercial effectiveness. Thus, to bring about improvement in the electrochemical performance of MnO2‐based supercapacitors, novel designs of MnO2 nanomaterials through composite formations with other substances, such as nanocarbons, conducting polymers or inorganic materials, namely metal oxides and sulfides, have been comprehensively explored. Extensive studies on these MnO2 binary nanocomposites revealed significant improvement in the electrochemical features compared to pristine phases; nevertheless, the achieved state is still far below practicability. Hence, scientists have opted for MnO2‐based ternary nanocomposites achieved by blending appropriate proportions the three components (MnO2 nanostructures being one of the constant components) that would promote synergism to attain suitable dimensions, crystallinity, crystal structure, conductivity, mass loading, and electrolyte selectivity so as to confer superior capacitance, charge transfer kinetics, better utilization of electroactive materials, energy and power densities, as well as improved mechanical stability and environmental adaptability. Herein, recent developments and advancements in the research of various MnO2‐based ternary nanocomposites employed for supercapacitor applications have been discussed and are compared with binary analogs with special emphasis on correlating their composition, morphology, and the electrochemical properties that are noticeably modified upon introduction of the third component. The associated challenges encountered in their progress toward commercialization and the probable ideas of persuading better strategic designs of these ternary systems for high‐performance supercapacitor applications have also been delineated.
Aiming for life cycle sustainability assessment of cement‐based composites: A trend study for wall systems of carbon concrete: Dresden Nexus Conference 2020—Session 4—Circular economy for building with secondary construction materials to minimise resource use and land use
Authors: Scope Christoph , Guenther Edeltraud , Schütz Julia , Mielecke Torsten , Mündecke Eric , Schultze Konstantin , Saling Peter 
Published: November 2020

AbstractThe narrative sustainable building is core message of European politics. Strategies discussed in this regard concern above all material minimization by using new more efficient manufacturing technologies, new form finding approaches for load‐bearing structures or new high‐performance materials. The goal of the research project V2.10, funded by the German Federal Ministry of Education and Research, refers to the latter by assessing the sustainability potential of building components made of innovative textile reinforced concrete. We conceptualized a life cycle sustainability assessment framework and applied it to variants of sandwich wall systems made of carbon concrete composites and steel‐reinforced concrete. Results indicate hotspots in technology and material choices that could be addressed by circular strategies, for example, refuse, reduce or recycling. Overall, one design variant made of carbon concrete composites is the best performing with respect to all dimensions of sustainability.
Ultrasound and air‐disturbance‐based enhancement of spiral microchannel extraction of Cu2+
Authors: Yang Xingdong , Xiao Wenqiang , Dai Shuang , Qu Jiyan , Luo Jianhong 
Published: November 2020

AbstractIn this work, we evaluated the enhancement effect on the extraction of Cu2+ from water by introducing ultrasound and air disturbance in a spiral microchannel device. We used 2‐ethylhexyl phosphonic acid mono‐2‐ethylhexyl ester (P507) as an extractant and used kerosene as a solvent. The effects of flow rate, temperature, initial pH of water, organic/aqueous phase ratio (O/A), the concentration of extractant, and the inner diameter of the pipe on the extraction of Cu2+ were investigated in liquid‐liquid micro‐extraction (LLME), ultrasonic micro‐extraction (UME), and liquid‐liquid‐gas micro‐extraction (LLGME). We found that, at a similar extraction rate, the extraction capacity per unit of time of UME was five times that of LLME, and the extraction capacity per unit of time of LLGME was 10 times that of LLME. By comparing the effects of three methods on extracting Cu2+, we followed the following priority order: LLGME > UME > LLME. The results of three‐stage extraction under optimum conditions were also studied. The results revealed that the highest extraction rate of the three methods could reach about 92% with the processing time of 72 seconds under optimal conditions.
Influence of lignin structure change during extrusion on properties and recycling of lignin‐polyethylene thermoplastic composites
Authors: Tang Yong , Jean Michel , Pourebrahimi Sina , Rodrigue Denis , Ye Zhibin 
Published: November 2020

AbstractKraft lignin has been widely investigated for the production of composite plastics by blending with different synthetic polymers. In the present study, softwood kraft lignin‐polyethylene (PE) composites are produced by extrusion. The change in lignin structure during the extrusion has been investigated and the influences of the lignin structure change on the properties and recyclability of the composites have been investigated. Extrusion reduces the content of functional groups in lignin (especially aromatic OH group) and decreases its glass transition temperature, but increases its weight‐average molecular weight and polydispersity index. In the lignin‐PE composites, lignin and PE only blend at the macroscopic level and there is no intermolecular interaction between them. Because extrusion reduces Tg and particle size of lignin, a lower temperature is needed to reprocess the lignin‐PE composites. These recycled composites have similar thermal properties, but enhanced mechanical properties. This work shows that lignin can be used as a potential component to reduce the usage of synthetic polymers, thus partially eliminating white pollution.