bluuebunny/crossref_metadata_2025_split huggingface dataset:
YAP Enhances Tumor Cell Dissemination by Promoting Intravascular Motility and Reentry into Systemic Circulation
Authors: Benjamin David C. , Kang Joon Ho , Hamza Bashar , King Emily M. , Lamar John M. , Manalis Scott R. , Hynes Richard O. 
Published: June 2020

Abstract

The oncogene YAP has been shown previously to promote tumor growth and metastasis. However, how YAP influences the behavior of tumor cells traveling within the circulatory system has not been as well explored. Given that rate-limiting steps of metastasis are known to occur while tumor cells enter, travel through, or exit circulation, we sought to study how YAP influences tumor cell behavior within the circulatory system. Intravital imaging in live zebrafish embryos revealed that YAP influenced the distribution of tumor cells within the animal following intravenous injection. Control cells became lodged in the first capillary bed encountered in the tail, whereas cells overexpressing constitutively active YAP were able to travel through this capillary plexus, reenter systemic circulation, and seed in the brain. YAP controlled transit through these capillaries by promoting active migration within the vasculature. These results were corroborated in a mouse model following intravenous injection, where active YAP increased the number of circulating tumor cells over time. Our results suggest a possible mechanism whereby tumor cells can spread to organs beyond the first capillary bed downstream from the primary tumor. These results also show that a specific gene can affect the distribution of tumor cells within an animal, thereby influencing the global pattern of metastasis in that animal.

Significance:
These findings demonstrate that YAP endows tumor cells with the ability to move through capillaries, allowing them to return to and persist in circulation, thereby increasing their metastatic spread.
See related commentary by Davidson, p. 3797
Observations on breeding behaviour of a pair of endangered Egyptian Vultures Neophron percnopterus (Linnaeus, 1758) over three breeding seasons in the plains of Punjab, India
Authors: Charn Kumar  , Amritpal Singh Kaleka  , Thind Sandeep Kaur 
Published: June 2020

The present study has been conducted to document information on breeding behaviour of Egyptian Vultures Neophrons percnopterus from Punjab. This study is based on 688 hours of video records documenting breeding behaviour of a pair of endangered Egyptian Vultures Neophrons percnopterus occupying the same nesting site over three consecutive breeding seasons from 2015 to 2017. The site is located in the hollow of a ventilation window of the Space Observatory in Punjabi University, Patiala, Punjab. During the third breeding period (February to August 2017), the nest activity has been extensively video-recorded in egg laying and incubation period, and chick rearing period using a Dome CCTV Camera. Both parents participated in nest building, and of the total recorded incubation time of 339.39h over 23 days the nest was attended for 199.35 and 139.46h by the female and male respectively, and unattended for 0.58h. The incubation period was 42 to 43 days, and the egg laying/hatching intervals between eggs/chicks was five days. A total of six young ones hatched and fledged from three broods of two eggs each. All chicks survived to fledging and no mortality or siblicide of younger chick occurred due to aggression/starvation by elder chick. The high fledging success rate indicates a healthy habitat and food source in the nesting area.
A checklist of angiosperm flora of low elevation lateritic hills of northern Kerala, India
Authors: K.A. Sreejith , V.B. Sreekumar , P. Prashob , S. Nita , M.P. Prejith , M.S. Sanil 
Published: June 2020

An inventory to prepare the checklist of angiosperm species in the lateritic hillocks of northern Kerala was conducted in five sampling sites during April 2013–March 2015. In total, we recorded 364 genera with 535 species, of which 334 are native and 201 are non-native. Native species were represented in 102 families, namely, Poaceae (28), Fabaceae (25), Acanthaceae (22), Rubiaceae (17) Euphorbiaceae (14), Commelinaceae (11), Phyllanthaceae (7), etc., whereas, non-native species were represented in 99 families. Among the native species herbs are the predominant habit with 147 species (44%). Out of the 72 endemic species, three taxa namely, Syzygium travancoricum, Santalum album and Hopea ponga are red listed species documented from the study area. Twenty-seven invasive species were also recorded and major threats to the laterite ecosystems are by Lantana camara, Mikania micrantha, Pennisetum polystachyon, Ipomoea spp., and Senna spp. Most part of the laterite has been converted to plantations, building sites and mining sites. The indiscriminate mining for laterite, soil and demolishing the hillocks have severely threatened the very existence of the flora.
Present status of the genus Sphrageidus Maes, 1984 (Lepidoptera: Erebidae: Lymantriinae) from India
Authors: Kaleka Amritpal Singh , Singh Devinder , Bali Gagan Preet Kour 
Published: June 2020

The surveys to different localities of Himachal Pradesh, Jammu & Kashmir and Uttarakhand yielded three species of genus Sphrageidus Maes, namely S. similis (Fuessly), S. simlensis (Gupta) and S. xanthorrhoea (Kollar) of subfamily Lymantriinae. The external morphological characters particularly wing maculation and venation along with genitalia characteristics have been studied and illustrated in detail. The male genitalic features like distinct saccus, ring-like juxta, simple valva, aedeagus with a hook or reversed spine at the apex and distinct wing venation, i.e., absence of vein M2 in hindwing completely conform the characterization of the genus. In the present studies, the species simlensis has been placed under genus Sphrageidus Maes making a new combination as Sphrageidus simlensis (Gupta) for its proper placement. The genus diagnosis has also been updated. The external morphological characters including wing maculation, venation and particularly the genitalic features proved significant from taxonomic point of view in all the three species.
Early stages of Nilgiri Grass Yellow Eurema nilgiriensis (Yata, 1990) (Lepidoptera: Pieridae), with a note on its range extension in the Kerala part of the Western Ghats, India
Authors: Valappil Balakrishnan , V.K.Chandrasekharan  
Published: June 2020

Complete documentation of the early stages of Eurema nilgiriensis (Yata) on the larval host plant Ventilago bombaiensis (Rhamnaceae) is presented in this paper. In addition to this, notes on the range extensions of this species in southern Western Ghats in Kannur, Kozhikode, Wayanad, Malappuram, and Palakkad districts in Kerala is also provided. Field records from the northern Kerala part of the Western Ghats and the complete biology are reported for the first time since its description in 1990. Photographic records from seven locations since 2011 were confirmed by comparing with the original descriptions and in consultation with the author of the species. Eggs collected from field were reared at home, and every stage observed is also reported, as well as the discovery of the host plant Ventilago bombaiensis. It is concluded that the species is widespread in forested areas at elevations from 70m to 1,000m.
In-Hospital Delays in Stroke Thrombolysis
Authors: Darehed David , Blom Mathias , Glader Eva-Lotta , Niklasson Johan , Norrving Bo , Eriksson Marie 
Published: June 2020

Background and Purpose:
Intravenous thrombolysis is a well-established treatment for acute ischemic stroke. Our aim was to quantify the effect of each minute delay in door-to-needle time (DNT) on 90-day survival, intracerebral hemorrhagic complication <36 hours, and functional outcomes at 3 months, in routine clinical practice.

Methods:
Our nationwide registry-based study included 14 132 adult patient admissions with ischemic stroke receiving intravenous thrombolysis from 2010 to 2017. Outcomes were analyzed using multivariable logistic regression, adjusting for potential confounders.

Results:
Median DNT was 47 minutes, with an improvement from 65 to 38 minutes during the study. Median age was 74 years, and median National Institutes of Health Stroke Scale 8 points. We found a significant impact of each minute delay in DNT with reduced odds of survival by 0.6%, increased odds of intracerebral hemorrhagic and worse activities of daily living by 0.3%, and worse living conditions and mobility by 0.4%.

Conclusions:
Improving DNT is a key factor in achieving good outcomes after stroke. We estimate that in Sweden alone in 2017, compared with 2010, the shorter DNT achieved have saved 38 lives, avoided 8 intracerebral hemorrhagic transformations, and spared, respectively, 36, 51, and 52 patients from a worsening in activities of daily living, living conditions, and mobility. DNT is sensitive for interventions and should be targeted in quality improvement efforts.
Comparing ‘Religion’ and ‘Nonreligion’: towards a Critique of Modernity
Authors: Horii Mitsutoshi 
Published: June 2020

Abstract
This essay starts with reference to “grapefruits” in Oliver Freiberger’s (2019) Considering Comparison and to “apples” and “oranges” in Bruce Lincoln’s (2018) Apples and Oranges: Explorations In, On and With Comparison. It disagrees with Freiberger when he compares “grapefruits” with some generic categories in Religious Studies including “shrine.” The category of “shrine” resembles more “fruits,” for example, because two shrines could have completely different genealogies, just like apples and oranges, but still belong to the same generic category. Then, the essay compares the categories of “religion” and “tree.” The boundary between “religion” and “nonreligion” is as arbitrary as that of “tree” and “non-tree.” At the same time, “religion” and “nonreligion” share common characteristics just like “tree” and “non-tree” do. Given this, it concludes with the suggestion that, when the “religiousness” of ostensibly “nonreligious” modernity is articulated, the category “religion” functions as a useful rhetorical tool to subvert modernity’s claim of universality and factual reality.
Towards an Understanding of Children’s Perceptions of Physical Punishment in the Family Context
Authors: Walton Brooke , J. Saunders Bernadette 
Published: June 2020

This qualitative synthesis explores how children perceive physical punishment in the family context. A search of four online databases identified eight qualitative studies. Findings revealed that children provided detailed accounts about their experiences of physical punishment, and they associated physical punishment with both emotional and physical pain. Children justified the use of physical punishment based on their precipitating behaviour, their status, and the intergenerational transmission of physical punishment. Children suggested alternative discipline to physical punishment, and urged caregivers to respect them. Children also recognised that physical punishment escalated in severity and negatively influenced the parent-child relationship. The results highlight the benefit of including children in research, the need to understand the factors that shape children’s perspectives and, most importantly, the ongoing influence of the United Nations Convention on the Rights of the Child (uncrc) (1989), to ensure that children’s human rights are recognised, and more widely upheld worldwide.
A new Cymothales Gerstaecker from the Gabonese rainforest (Neuroptera Myrmeleontidae)
Authors: BADANO DAVIDE 
Published: June 2020

Nine species of Cymothales, one of the most characteristic African antlion genera, are known from the Guineo-Congolian rainforests. A new species, Cymothales massaronei sp. nov. is described here from Gabon. Cymothales massaronei sp. nov. is characterized by the fifth tarsomere equal in length to the first tarsomere, but differs from all of the congeners with this character in the shape and markings of wings, shape of antenna and body pattern.
Establishing reference values for the pubofemoral distance in normal infant medial hips by ultrasound
Authors: Ban Yongguang , Luan Qinhua , Shi Miao , Sun Bo , Li Tianyou , Wang Yanzhou , Lin Xiangtao , Teng Jianbo 
Published: June 2020

Background Measuring the pubofemoral distance (PFD) is important for evaluating the effectiveness of hip reduction in the treatment of developmental dysplasia of the hip (DDH). However, reference PFD values have not been established in normal infants. Purpose To investigate variations in PFD values measured in normal infant medial hips stratified by age, laterality, and gender. Material and Methods A total of 240 infants diagnosed with Graf type Ia and/or Graf type Ib hips by ultrasonography were stratified into eight age groups: 0–1 month; 1–2 months; 2–3 months; 3–4 months; 4–5 months; 5–6 months; 6–7 months; and 7–12 months. The bilateral medial hips were scanned with transinguinal ultrasound. The PFD was defined as the distance between the lateral edge of the superior ramus of pubic bone and the medial edge of the femoral head. Inter-observer reproducibility was assessed. Results Among the 240 infants, there were 371 Graf type Ia hips and 109 Graf type Ib hips. Mean ± SD bilateral PFD values of eight groups were measured separately. There were no significant differences in mean PFD values for left or right hips (t = 0.946, P = 0.345) or mean bilateral PFD values in male and female infants (t = 1.445, P = 0.149). Mean PFD values increased linearly with age (left: r = 0.680, P < 0.0001; right: r = 0.682, P < 0.0001). Inter-observer reproducibility was excellent. Conclusion This study established reference PFD values from the medial hip in infants aged 0–12 months. PFD values increased with age, but were not significantly influenced by laterality or gender. These data provide detailed information that can support follow-up of infants treated for DDH.
Micro RNA-363 inhibits esophageal squamous cell carcinoma progression by directly targeting sperm-associated antigen 5
Authors: Zhang Lingmin , Wang Li , Lu Ning , Wang Jia , Yan Rong , Yan Honglin , Zhang Jia , Zhang Mingxin 
Published: June 2020

Objective Micro RNA (miR)-363 has many important biological functions in cancers, but its roles in esophageal squamous cell carcinoma (ESCC) remain unclear. Methods We used reverse transcription PCR to quantify the expression of miR-363 in 80 ESCC tissues and analyzed its relationship with clinicopathological factors and overall survival. The effects of miR-363 on cell proliferation, apoptosis, and invasion were detected using the MTT assay, flow cytometry, and Transwell invasion assays, respectively. Further, we investigated the post-transcriptional regulation of sperm-associated antigen 5 (SPAG5) expression by miR-363 using luciferase reporter assays. Finally, the effects of SPAG5 on miR-363 were studied by SPAG5 overexpression. Results miR-363 expression was decreased in both ESCC specimens and cell lines, compared with controls, and correlated with lymph node metastasis and tumor differentiation. Low miR-363 expression was identified as an independent prognostic factor for ESCC. miR-363 overexpression decreased ESCC cell proliferation and invasion and increased apoptosis, while the opposite was seen after miR-363 inhibition. Moreover, SPAG5 was identified as a direct target of miR-363, and the reintroduction of SPAG5 restored miR-363-induced effects. Conclusions miR-363 acts as a tumor suppressor by post-transcriptionally regulating SPAG5 expression, suggesting its potential as a diagnostic biomarker and therapeutic target for ESCC.
The effectiveness of a modified advance care planning programme
Authors: Deng Renli , Zhang Jianghui , Chen Liuliu , Miao Jiarui , Duan Jiazhong , Qiu Yeyin , Leung Doris , Chan Helen , Lee Diana TF 
Published: June 2020

Background: Frailty is a natural consequence of the aging process. With the increasing aging population in Mainland China, the quality of life and end-of-life care for frail older people need to be taken into consideration. Advance Care Planning has also been used worldwide in long-term facilities, hospitals and communities to improve the quality of end-of-life care, increase patient and family satisfaction, and reduce healthcare costs and hospital admissions in Western countries. However, it has not been practiced in China. Research objective: This study aimed to evaluate the effectiveness of a modified Advance Care Planning intervention in certainty of end-of-life care, preferences for end-of-life care, quality of life concerns, and healthcare utilization among frail older people. Research design: This study used a quasi-experimental design, with a single-blind, control group, pretest and repeated posttest approach. Participants and research context: A convenience sample of 74 participates met the eligibility criteria in each nursing home. A total of 148 frail older people were recruited in two nursing homes in Zhejiang Province, China. Ethical considerations: The study received ethical approval from the Clinical Research Ethics Committee, the Faculty of Medicine, and The Chinese University of Hong Kong, CREC Ref. No: 2016.059. Findings: The results indicated the Advance Care Planning programme was effective at increasing autonomy in decision making on end-of-life care issues, decreasing decision-making conflicts over end-of-life care issues, and increasing their expression about end-of-life care. Discussion: This study promoted the participants’ autonomy and broke through the inherent custom of avoiding talking about death in China. Conclusion: The modified Advance Care Planning intervention is effective and recommended to support the frail older people in their end-of-life care decision in Chinese society.
Airway managed by emergency physicians or anaesthesiologists in trauma patients: A retrospective cohort analysis of outcomes
Authors: Tang Tsz Ha , Yang Marc LC , Chan On Yee , Chan Lily PS , Ho Hiu Fai 
Published: June 2020

Objectives: In some trauma centres, anaesthesiologists have the primary responsibility of managing airway in trauma resuscitation. However, as emergency physicians establish a separate specialty with airway management and endotracheal intubation being one of the core skills, role delineation within trauma members may vary. In this cohort study, we aim to determine the difference in mortality of trauma patients requiring intubation in the emergency department between emergency physicians and anaesthesiologists. Methods: We screened all 1588 patients in the hospital trauma registry from 2015 to 2018. We included all patients requiring endotracheal intubation and aged 18 or above but excluded those with pregnancy, presented with cardiac arrest and secondarily transferred from other hospitals. A total of 349 eligible patients were sorted into two cohorts according to the physicians who performed intubations (anaesthesiologists = 205 patients, emergency physicians = 144 patients). Patients’ baseline demographics, 30-day all-cause mortality and other predefined secondary outcomes were compared by statistical tests. Stepwise logistic regression of 30-day all-cause mortality was performed. Results: Our study has shown that intubation by emergency physicians was not associated with higher 30-day all-cause mortality after potential confounders were controlled by logistic regression (adjusted odds ratio = 1.253, p = 0.607). Both groups also did not differ in other clinical important secondary outcomes, including proportion of successful intubations, use of surgical airway or rescue manoeuvres, respiratory and airway complications, mortality in intensive care or high-dependency unit, post-intubation cardiac arrest, post-intubation hypotension and post-intubation hypoxia. Conclusion: Endotracheal intubation by emergency physicians is not associated with increased 30-day all-cause mortality when compared to anaesthesiologists after accounting for confounders.
Partisan Politics and Public Education: Finding the Formula for (Electoral) Success
Authors: Kitchens Karin E. 
Published: June 2020

AbstractDo state politicians reward school districts that vote in favor of the party in power more than school districts that vote in favor of the opposing party? With large shares of money at the state level to transfer to local governments and the ability to target core voters, it would seem likely that politicians would take advantage of the ability to distribute education funds. However, in understanding how states distribute education funds, little emphasis is given to partisan influences, particularly the congruence between local school districts and the state level. To test this, I collected data at the precinct level within each state and, using mapping software, spatially joined precinct boundaries to school district boundaries. Once this relationship was established, I aggregated precinct-level information to school districts to understand the partisan voting patterns within each school district for elections from 2000 to 2010. This article finds evidence that funding formulas are susceptible to political influence and that parties are able to influence the geographic distribution of education funds to core voters.
The Erdmannshöhle near Hasel, SW Germany: karst environment and cave evolution
Authors: Becker Arnfried , Piepjohn Karsten , Schröder-Ritzrau Andrea 
Published: June 2020

AbstractThe Erdmannshöhle is located at the NE margin of the Dinkelberg plateau in SW Germany. With a length of 2315 m, it is the longest cave in the deep open karst area near the village of Hasel. Three main cave levels developed in moderately SW-dipping, thinly bedded and fractured limestones of the Upper Muschelkalk (Triassic). The youngest cave level containing the cave stream is still active. Eighteen samples for U/Th dating were taken from the oldest and the intermediate cave levels. At the oldest cave level, the ages range from 162 to 110 ka, indicating speleothem growth starting in the middle Beringen Glaciation and terminating at the end of the Eem Interglacial. At the intermediate cave level, the ages range from 100 to 12 ka, i.e. early Birrfeld Glaciation to Younger Dryas Stadial. The age dating shows that speleothem growth did not cease completely during long periods of harsh climate conditions during the Beringen and Birrfeld glaciations and that permafrost terminating speleogenesis and speleothem growth was thus probably established only temporarily over relatively short periods. A conceptual model for the Middle Pleistocene to Holocene development of the Erdmannshöhle is presented within the framework of modern Quaternary lithostratigraphy. This model facilitates a first correlation of the cave evolution with the Middle to Late Pleistocene depositional record in the Möhlinerfeld, which is a key area for modern Quaternary lithostratigraphy in Switzerland and Southern Germany. Thus, speleological and Quaternary research, both based on a regional scale data compilation, complement one another with respect to the timing of events and the landscape evolution during the Quaternary and Late Neogene.