bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ligand and Structure-based Virtual Screening of Lamiaceae Diterpenes with Potential Activity against a Novel Coronavirus (2019-nCoV)
Authors: Rodrigues Gabriela Cristina Soares , dos Santos Maia Mayara , de Menezes Renata Priscila Barros , Cavalcanti Andreza Barbosa Silva , de Sousa Natália Ferreira , de Moura Érika Paiva , Monteiro Alex France Messias , Scotti Luciana , Scotti Marcus Tullius 
Published: July 2020

Background:
The emergence of a new coronavirus (CoV), named 2019-nCoV, as an outbreak
originated in the city of Wuhan, China, has resulted in the death of more than 3,400 people this
year alone and has caused worldwide an alarming situation, particularly following previous CoV epidemics,
including the Severe Acute Respiratory Syndrome (SARS) in 2003 and the Middle East Respiratory
Syndrome (MERS) in 2012. Currently, no exists for infections caused by CoVs; however, some
natural products may represent potential treatment resources, such as those that contain diterpenes.

Objective:
This study aimed to use computational methods to perform a virtual screening (VS) of candidate
diterpenes with the potential to act as CoV inhibitors.

Methods:
1,955 diterpenes, derived from the Nepetoideae subfamily (Lamiaceae), were selected using
the SistematX tool (https://sistematx.ufpb.br), which were used to make predictions. From the ChEMBL
database, 3 sets of chemical structures were selected for the construction of predictive models.

Results:
The chemical structures of molecules with known activity against SARS CoV, two of which
were tested for activity against specific viral proteins and one of which was tested for activity against the
virus itself, were classified according to their pIC50 values [-log IC50 (mol/l)].

Conclusion:
In the consensus analysis approach, combining both ligand- and structure-based VSs, 19
compounds were selected as potential CoV inhibitors, including isotanshinone IIA (01), tanshinlactone
(02), isocryptotanshinone (03), and tanshinketolactone (04), which did not present toxicity within the
evaluated parameters.
Activation of Myosin Phosphatase by Epigallocatechin-Gallate Sensitizes THP-1 Leukemic Cells to Daunorubicin
Authors: Tóth Emese , Erdődi Ferenc , Kiss Andrea 
Published: July 2020

Background:
The Myosin Phosphatase (MP) holoenzyme is composed of a Protein Phosphatase type 1 (PP1) catalytic subunit
and a regulatory subunit termed Myosin Phosphatase Target subunit 1 (MYPT1). Besides dephosphorylation of myosin, MP has been
implicated in the control of cell proliferation via dephosphorylation and activation of the tumor suppressor gene products, retinoblastoma
protein (pRb) and merlin. Inhibition of MP was shown to attenuate the drug-induced cell death of leukemic cells by chemotherapeutic
agents, while activation of MP might have a sensitizing effect.

Objective:
Recently, Epigallocatechin-Gallate (EGCG), a major component of green tea, was shown to activate
MP by inducing the dephosphorylation of MYPT1 at phospho-Thr696 (MYPT1pT696), which might confer enhanced
chemosensitivity to cancer cells.

Methods:
THP-1 leukemic cells were treated with EGCG and Daunorubicin (DNR) and cell viability was analyzed.
Phosphorylation of tumor suppressor proteins was detected by Western blotting.

Results:
EGCG or DNR (at sub-lethal doses) alone had moderate effects on cell viability, while the combined
treatment caused a significant decrease in the number of viable cells by enhancing apoptosis and decreasing
proliferation. EGCG plus DNR decreased the phosphorylation level of MYPT1pT696, which was accompanied by
prominent dephosphorylation of pRb. In addition, significant dephosphorylation of merlin was observed when
EGCG and DNR were applied together.

Conclusion:
Our results suggest that EGCG-induced activation of MP might have a regulatory function in mediating the
chemosensitivity of leukemic cells via dephosphorylation of tumor suppressor proteins.
Angiotensin-(1-7) Improves Islet Function in a Rat Model of Streptozotocin- Induced Diabetes Mellitus by Up-Regulating the Expression of Pdx1/Glut2
Authors: Li Jingjing , Zhu Ruifang , Liu Yalin , Yang Jinhui , Wang Xiaoyan , Geng Lisha , Xu Tingting , He Junhua 
Published: July 2020

Objective:
To observe the effects of angiotensin-(1-7) (Ang-(1-7)) on glucose metabolism,
islet function and insulin resistance in a rat model of streptozotocin-induced diabetes mellitus (DM)
and investigate its mechanism.

Methods:
Thirty-four male Wistar rats were randomly divided into 3 groups: control group, which was
fed a standard diet, DM group, high-fat diet and injected with streptozotocin, and Ang-(1-7) group
receiving an injection of streptozotocin followed by Ang-(1-7) treatment. Blood glucose level, fasting
serum Ang II and insulin levels, and homeostasis model assessment of insulin resistance (HOMA-IR)
were measured. The pancreases were collected for histological examination, protein and gene expression
analysis.

Results:
Compared with the control group, fasting blood glucose, serum angiotensin II level, and
HOMA-IR value increased, while serum insulin level decreased in the DM group. Moreover, islet
structure was damaged, β cells were irregularly arranged, the cytoplasm was loose in the DM group.
Expressions of Pancreatic duodenal homeobox-1 (Pdx1), glucose transporter-2 (Glut2) and glucokinase
(Gk) were significantly decreased in the DM group compared with the control group. However, the
DM-associated changes were dramatically reversed following Ang-(1-7) treatment.

Conclusion:
Ang-(1-7) protects against streptozotocin-induced DM through the improvement of insulin
secretion, insulin resistance and islet fibrosis, which is associated with the upregulation of Pdx1,
Glut2 and Gk expressions.
Exploring the Effect of the Number of Characteristic Groups in Melaminebased Polymers on the Photocatalytic Performance
Authors: Jiang Zhenhua , Ni Cailing , Zhou Yubing , Qin Yuancheng 
Published: July 2020

:
Three polymers containing different numbers of thiophene groups were constructed. Degradation
experiments on the aqueous solutions of tetracycline and norfloxacin revealed that the polymer
with three thiophene groups in the monomer indicated the best degradation efficiency of
73.7% for tetracycline and 56.9% for norfloxacin. Moreover, this polymer had a relatively stronger
ability to separate and transport photocharging carriers under visible light. Therefore, the photocatalytic
performance of conjugated polymers could be regulated by changing the number of characteristic
groups.

Background:
Antibiotic residues in the environment are considered as one of the most serious
sources of environmental pollution. Although catalyst photodegradation is regarded as the most
promising strategy to solve environmental pollution-related problems, it still requires new and
advanced photocatalysts.

Objective:
To design new organic conjugated material structures.

Material and Methods:
Three polymers (ThME-1, ThME-2, and ThME-3) were prepared by the
condensation of melamine with 2, 5-thiophenedicarboxaldehyde, thieno[3, 2-b]thiophene-2,
5-dicarbaldehyde, and dithieno[3, 2-b:2’, 3’-d]thiophene-2, 6-dicarbaldehyde. The photocatalytic
performance of these polymers was investigated by testing their diffused light absorption capacity,
photocurrent response, AC impedance, specific surface area, fluorescence, and thermal stability.

Results:
ThME-3, containing three thiophene groups in the monomer, manifested the best degradation
efficiency of 73.7% for tetracycline and 56.9% for norfloxacin.

Conclusion:
The photocatalytic performance of conjugated polymers could be regulated by changing
the number of characteristic groups.
'Authority is everything': A study of the politics of textual ownership
Authors: Thompson Celia 
Published: July 2020

The relationships between text, knowledge and power in the construction of writer identities have been the source of much debate among philosophers,literary scholars and language educators (e.g., Bakhtin 1981; Kristeva 1986;
Pennycook 2001). Such interrelationships are played out in the transcultural encounters that occur between students and their lecturers on a daily basis in our contemporary university classrooms. This article explores how two
undergraduate students from different language backgrounds, studying at a major Australian university, struggle to deal with the politics of text/knowledge construction in their quest for a sense of ownership and authorial control over the assignments they produce. Data consisting of interviews and assignments by students from different disciplinary fields have been selected from a larger study into plagiarism and intertextuality. Drawing on the work of Bakhtin (1981),
Kristeva (1986) and Howard (1992; 1995; 1999), my analysis indicates firstly, that students feel constrained by the homogenising (Holton 2000) forces of the academic writing conventions that they feel obliged to imitate; and secondly,
confused by unified and autonomous concepts of authorship and originality that fail to take account of the 'shared' nature of the processes of text/knowledge production. I conclude by suggesting that the development of writer identity needs to be seen as part of the ongoing epistemological negotiations that occur between students and lecturers as they struggle to construct desired meanings across texts. I then consider the implications of these findings for academic writing pedagogy.
Taking the Mountain to Mohammed: Transitioning International Graduate Students into Higher Education in Australia
Authors: Handa Neera , Fallon Wayne 
Published: July 2020

This paper reports on findings of evaluative research into student perceptions of a structured academic development workshop, which was specifically designed to induct and orient international students into the academic expectations of their program of study at a university in Australia. With most Australian universities engaged in the business of internationalisation of higher education, there is some debate about the adequacy of practices adopted by these institutions to familiarise their non English-speaking background (NESB) international students with the Australian academic culture. While the practices of some Western universities are sometimes said to be inadequate, there also appears some consternation about international students’ lack of motivation to learn and their inability to master Western academic conventions. Against this backdrop, the paper outlines the impetus for collaboration between the university’s Learning Skills Unit and faculty staff in
designing and facilitating a tailored academic development workshop for graduate students. After laying out related literature and details of the workshop, there is discussion of the data collection methods, and an analysis of the data from students. The paper makes a call for repeat workshops at the beginning of every semester, as an indispensable component of the overall content delivery strategies in the faculty’s graduate program. The paper concludes by contemplating the educational integrity inherent in program and faculty staff development initiatives, which are focused on addressing the academic and cultural proclivities of an international student cohort.
A Review on Life Cycle Management Approach on Asset Qualification
Authors: Mali Mamta R. , Bhusnure O.G. , Mule Shrikrishna T. , Waghmare S.S. 
Published: July 2020

All equipment’s used in the production of products shall be properly Validated, Qualified and Calibrated to demonstrate that it is suitable for its intended purpose. Qualification is an important aspect of the pharmaceutical quality system. When the equipment is properly qualified, verified and maintained, there is the possibility of Consistent performance of the equipment. A well designed qualification program saves valuable time and cost. Qualification is called a cyclic process because it is a never ending process. Appropriate documentation of the qualification program is very important as lack of the documented evidence does not give any meaning to qualification (Not documented it means not done). The current programs and procedures of equipment qualification used within any pharmaceutical and bioscience industry are based on ‘regulatory requirements’, ‘voluntary standards’, ‘vendor practices’, and ‘industry practices’. The output is considerable variation in the way any pharmaceutical and biotechnological companies approach for the laboratory equipment. The lifecycle management approach of equipment qualification covers entire life cycle for the specification, design, manufacturing, installation, commissioning, qualification (4Qs Model DQ, IQ, OQ, PQ), operation & maintenance of equipment in a risk based life cycle management approach. The goal of any regulated pharmaceutical and bioscience company is to provide reliable and valid data suitable for its intended purpose. Main goal of equipment qualification is to form the basis for written procedures for production and process control which are designed to assure that the drug products have the SISPQ (Safety, Identity, Strength, Purity and Quality)
Keywords: Validation, Calibration, Life cycle management approach, Qualification (4Qs Model- DQ, IQ, OQ & PQ), SISPQ (Safety, Identity, Strength, Purity and Quality)
Diabetes Insipedes in a child disclosing localized Langerhans’ Cell Histiocytosis
Authors: El-Reshaid Kamel , Al-Bader Shaikha , Markova Zaneta 
Published: July 2020

Langerhans cell histiocytosis (LCH) is monoclonal neoplastic condition of aberrant bone marrow histiocytes. The latter are part of the innate immune system and certain exogenous/endogenous stimuli may trigger its expansion. Hence LCH can present with limited or multiple organ involvement that may include; bones, lung, endocrine, skin, lymph nodes, spleen and bone marrow. In this case report, we describe a 3-year-old boy who presented with severe polyuria and polydipsia. Laboratory investigations were consistent with diabetes insipidus (DI). MRI of the brain; confirmed absence of the bright spot in his pituitary gland did not show evidence of tumor or enlargement by inflammation. Moreover, MRI revealed 2 skull lesions and their subsequent biopsy confirmed LCH. Systemic examination and tests including PET scan did not show additional lesions. Since his disease was localized, he received only Desmopressin acetate 120 ug twice daily for his DI without surgery, radiotherapy or chemotherapy. One year later, his disease remained limited to DI and the 2 bonny lesions.
Keywords: bone, diabetes insipidus, pituitary, desmopressin, Langerhans cell histiocytosis.
Hematologic malignancies in children: Epidemiological aspects in the pediatric oncology department of Oran Anti-Cancerous center, Algeria (2009-2013)
Authors: Bachir Bouiadjra Chahrazed , Seddiki Oum Kaltoum , Diaf Mustapha Bachir 
Published: July 2020

The incidence of the hematological malignacies, cancers of blood and lymphoid organs, has been in continuous increase for the last 20 years. In Algeria, few data on hematologic malignancies are available in the absence of a population register. The aim of this work is to describe the epidemiological aspect of hematologic malignancies in children from northwestern Algeria.
This study was carried out in 366 patients, with hematological malignancies, aged from 1 month to 15 years, over a period of 5 years (2009-2013). The study was carried out in the Anti-Cancerous Centre of Oran, Algeria.
We noted a predominance of male gender comparing to the females with a sex ratio M/F of 1.2. A male predominance was found for all pathologies (sex ratio of 1.08 for acute lymphoid leukemia, ALL) except for acute myeloid leukemia, AML where a female predominance was observed with a sex ratio F/M of 1.21. 0-3 years age group is the most affected by these haemopathies with 34.2%, however, patients older than 10 years are the least affected. The most frequent malignant haemopathies was the ALL with 60.9%, followed by the AML with 16.9%. The mortality rate in all the studied patients is about 8.2%. The mortality rate in patients with chronic myeloid leukemia (CML) was about 20%, which is significantly higher compared to those recorded in patients with ALL and LH (5.4% and 5%, respectively).
This study highlights the need for broader strategies for better understanding of all epidemiological aspects of childhood hematological malignancies and for adopting case management and prevention policies.
Keywords: Hematologic malignancies, epidemiological characteristics, children, acute lymphoblastic leukemia, Oran anti cancerous centre.
Gastroprotective effect and in vitro Antioxidant Activities of the Aqueous Extract from Artemisia absinthium L Aerial Parts
Authors: KAOUDOUNE Chahrazed , Benchikh Fatima , Benabdallah Hassiba , Loucif Karima , Mehlous Soulaf , Amira Smain 
Published: July 2020

Artemisia absinthium L. is a medicinal plant largely used in traditional medicine. The aim of this study was to estimate the content of polyphenols, and flavonoids compounds and also to evaluate the antioxidant and the anti-ulcer activities of the Aqueous extract from Artemisia absinthium L. aerial parts. The Folin-Ciocalteau and AlCl3 methods were applied in order to quantify the polyphenolic and flavonoids contents, respectively. However, DPPH method was used to evaluate the in vitro antioxidant activity. Quantitative analysis of the yield and phenolic content of the aqueous extract of Artemisia absinthium showed that the yield of the aqueous extract was 19.32% and its phenolic content was 58.66 ± 2.16 μg GAE / mg dry extract for polyphenols and 6.85 μg QE / mg dry extract for the flavonoids. The antioxidant activity of the plant extract evaluated by the DPPH test is very important (IC50=45.48±0.37 µg/ml). Treatment of mice with the aqueous extract of Artemisia absinthium at a dose of 400 mg / kg significantly reduced the ulcerogenic effect of ethanol on the gastric wall with an estimated protection rate of 91%. These findings suggest that Artemisia absinthium L. aqueous extract possessed good antiulcer and antioxidant potentials. This supports the traditional claims of this plant in folklore medicine.
Keywords: Artemisia absinthium, polyphenols, antioxidant activity, gastric ulcer, ethanol.
In Vitro Antimicrobial, Antiviral and Cytotoxicity Activities of Aspergillus oryzae Isolated From El-Baida Marsh in Algeria
Authors: Houda Sara Nacef , Rachid Belhattab , Julio Galvez , Jesus Rodriguez-Sojo María , Teresa Vezza 
Published: July 2020

This work covers the study of antimicrobial and antiviral activities of the Aspergillus oryzae strain isolated from saline soil (El-Baida marsh in Algeria). The crude extract obtained with ethyl acetate displayed antimicrobial activity against Gram-positive and Gram-negative bacteria and the yeast Candida albicans with a mean of 16.69 mm of inhibition zone and a minimal inhibitory concentrations MICs between 7.28 and 21.85 μgmL-1. We also assessed the antiviral activity against Herpes simplex-2 Virus (HSV-2), in which no inhibitory effect was exhibited. In addition, cytotoxicity activity was tested in Caco-2 and RAW 264, a human epithelial and a murine macrophage cell line, respectively, revealing a no-toxic effect of the extract. The studied isolate extract possesses an antimicrobial property and its non-toxicity to the host cells becomes very important, and can be exploited for the production of new pharmacological and biotechnological agents.
Keywords: Aspergillus oryzae, antimicrobial activity, antiviral activity, cytotoxicity, fungal extraction.
Estimasi Karakteristik Dinamis Bawah Permukaan Menggunakan Refraksi Mikrotremor di Kota Palu
Authors: Hendra Hendra , Efendi Rustan , Rusydi H. M. 
Published: July 2020

ABSTRAK Penelitian dengan metode refraksi mikrotremor (REMI) telah dilakukan di Kecamatan Palu Selatan, Kecamatan Palu Barat dan Kecamatan Palu Timur dengan merekam penjalaran gelombang yang terjadi di bawah permukaan. Adapun tujuan dari penelitan ini menggunakan variasi kecepatan rambat gelombang melalui metode refraksi mikrotremor (REMI). Pengolahan data dilakukan dengan menggunakan 2 program yaitu menggunakan program Surface Wave Analysis Wizard dan program Waveq. Hasil penelitian ini dilakukan di 3 lokasi yang berbeda. Lokasi pertama berada pada kawasan lapangan Kawatuna Kecamatan Palu Selatan dengan nilai kecepatan gelombang geser 282,0 m/s dan nilai periode dominan 0,42 s sehingga termasuk dalam klasifikasi jenis tanah IV. Lokasi kedua berada kawasan Masjid Agung Kecamatan Palu Barat dengam nilai kecepatan gelombang geser 215,0 m/s dan nilai periode dominan 0,55 s sehingga termasuk dalam klasifikasi jenis tanah IV. Lokasi ketiga berada di kawasan Mapolda Sulteng Kecamatan Palu Timur dengan nilai kecepatan gelombang geser 327,4 m/s dan nilai periode dominan 0,36 s sehingga termasuk dalam klasifikasi jenis tanah III.
Kata Kunci: Refraksi Mikrotremor (ReMi), Kecapatan gelombang geser, Periode dominan.
Fabrikasi dan Karakterisasi Briket Limbah Buah Pinus dengan Perekat Limbah Kulit Pohon Pinus
Authors: Linggi Murni , Ulum Syahrul , Darwis Darmawati 
Published: July 2020

Penelitian tentang fabrikasi dan karakterisasi briket limbah buah pinus dengan perekat limbah kulit pohon pinus telah dilakukan. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi limbah kulit pohon pinus sebagai perekat terhadap karakter briket tersebut. Proses pembuatan dimulai dengan melakukan karbonisasi limbah buah pinus dan penghalusan limbah kulit pohon pinus. Kedua bahan tersebut kemudian dicampur dan dicetak dengan metode cetak panas. Sampel briket yang dihasilkan dikarakterisasi untuk mengetahui kadar air, kadar abu, volatil matter, fixed carbon, nilai kalor, kuat tekan, lama penyalaan dan laju pembakaran. Hasil penelitian menunjukan sampel briket terbaik yaitu yang memiliki presentase campuran limbah kulit pohon pinus sebesar 37,5% dengan hasil pengujian kadar air 6,85%, kadar abu 3,99%, volatil matter 38,55%, fixed carbon 50,6%, nilai kalor 6.138 cal/gr, laju pembakaran 0,14 gr/detik,dan kuat tekan 526,1 kgf/ . Penambahan persentase perekat cenderung menambah kadar abu, fixed carbon, nilai kalor, kuat tekan, laju pembakaran dan cenderung menurunkan nilai kadar air, volatil matter, dan lama penyalaan. Hasil penelitian ini menunjukan bahwa limbah buah pinus dengan perekat limbah kulit pohon pinus layak digunakan sebagai bahan dalam karakterisasi biobriket
Kata Kunci: Limbah, Buah Pinus, Kulit Pohon Pinus, Briket
21ST CENTURY SKILLS OF ECONOMIC INTELLIGENCE RELATED TO THE RESILIENCE OF THE NATIONAL ECONOMY
Authors: Anis Aswhar , Susdarwono Endro Tri 
Published: July 2020

Indonesia is an agricultural country, but imports rice, sugar, wheat flour, maize, milk powder, onions, garlic, soybeans, carrots, from abroad. In all countries of the world, agriculture is the most protected sector of the economy. Food security is important. If a country imports most of its food products from other countries to fulfill its need because the price is lower than domestic prices, then the country will become dependent in its food supply on other countries for the future. This will be dangerous. So that 21st century skills are needed towards economic intelligence in Indonesia who can be relied on in terms of national economic resilience. These skills include : Ecological: the business environtment, Psycho-sociological: internal actors of organisation, Reticulated: external human networks claimed and moblized around the project, Teleological: final purpose of the approach through strategy development, Technological: includes all the methods, tools and techniques used for the entire process of decision support through surveillance process, information protection, or benchmarking. Economic intelligence concerns the set of concepts, methods and tools which unify all the co-ordinated actions of research, acquisition, treatment, storage and diffusion of information, relevant to individual or clustered enterprises and organisations in the framework of a strategy.