bluuebunny/crossref_metadata_2025_split huggingface dataset:
The microbiota of the surface, dermis and subcutaneous tissue of dog skin
Authors: García-Fonticoba Rocío , Ferrer Lluis , Francino Olga , Cusco Anna 
Published: August 2020

Abstract
Background. Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. This possibility, however, has not been investigated in the dog so far. The aim of this study was to determine if bacteria can be detected in the dermis and subcutaneous tissue of healthy dogs using two different approaches: traditional cultures and DNA sequencing of the V4 region of bacterial 16S rRNA gene using next-generation sequencing (NGS). Results. Seven healthy dogs were included in the study, and two sets of samples were collected from each subject. Sample sets were composed of a 6-mm abdominal skin biopsy, including epidermis, dermis, and subcutis, a skin surface swab, and an environmental blank sample for contamination control. One set of samples from each dog was submitted for bacterial culture and the other one for bacterial DNA amplification and sequencing. Five different bacterial genera (Staphylococcus, Bacillus, Corynebacterium, Streptococcus, and Enterococcus) were isolated in five out of the seven skin surface swab samples with aerobic microbiological culture methods, while no growth was obtained from the other two samples. Although some DNA could be amplified from epidermal, dermal, and subcutaneous tissue samples, the results of the NGS were similar to those of the blanks. Conclusion. When investigated with aerobic microbiological culture methods, the dermis and subcutaneous tissue of dogs are sterile. NGS techniques lead to the detection of some bacterial DNA, similar to the signal detected in blanks, which does not support the presence of a microbiota in dermis or subcutaneous tissue.
Self-Reported Psychological Problems and Coping Strategies: A Web-Based Study in Peruvian Population During the COVID-19 Pandemic.
Authors: Guerrero Rita Judith Ames , Parra Victoria Aymé Barreda , Cahua Julio Cesar Huamani , Holl Jane Banaszak 
Published: August 2020

Abstract
BACKGROUND: The Coronavirus pandemic has disrupted health systems across the world and led to major shifts in individual behavior by forcing people into isolation in home settings. Its rapid spread has overwhelmed populations in all corners of Latin-American countries resulting in individual psychological reactions that may aggravate the health crisis. OBJECTIVE: this study reports on demographics, self-reported psychological morbidities and associated coping styles during the COVID-19 pandemic for the Peruvian population. Methods: This cross-sectional study uses an online survey with snowball sampling that was conducted after the state of emergency was declared in Perú (on April 2nd). The General Health Questionnaire (GHQ -28) was used to identify somatic symptoms, incidence of anxiety/ insomnia, social dysfunction and depression and the Coping Strategy Questionnaire (COPE-28) mapped personal strategies to address recent stress. RESULTS: 434 self-selected participants ranging in age from 18 to 68 years old (ME =33.87) completed the survey. The majority of participants were women (61.3%), aged between 18 and 28 (41.7%), well-educated (>=85.0 %), Peruvian (94.2%), employed (57.4%) and single (71.20 %). 40.8% reported psychological problems, expressing fear of coronavirus infection (71.43%). Regression analysis shows that women developed more somatic symptoms (p<0.001*, C.I: -2.75 to -.99) and greater anxiety/insomnia (p=0.00*, C.I: -2.98 to 0.84) than men. Depression and social dysfunction were equally likely at any age. Educational status was protective against developing psychological disorders (p<0.05). While active responses (acceptance and social support) are scarcely used by individuals with psychological problems; passive strategies (such as denial, self-distraction, self-blame, disconnection, and emotional discharge) are more commonly reported.CONCLUSION: This study provides a better understanding of psychological disorders occurring during the COVID-19 pandemic in the Peruvian population. About half of the respondents reported psychological morbidity and poor coping responses. This evidence informs the need for broader promotional health policies focused on strengthening individual’s active strategies to improve mental health, especially in underprivileged groups during and after the Covid-19 pandemic.
Long noncoding RNA ARHGAP5-AS1 inhibits migration of breast cancer cell via stabilizes SMAD7 protein&nbsp;
Authors: Wang Chen-Long , Li Jing-Chi , Zhou Ci-Xiang , Ma Cheng-Ning , Wang Di-Fei , Wo Lu-Lu , He Ming , Chen Guo-Qiang , He Jian-Rong , Zhao Qian 
Published: August 2020

Abstract
Background: Tumor metastasis is the main cause of death from breast cancer patients and cell migration plays a critical role in metastasis. Recent studies have shown long non-coding RNAs (lncRNAs) play an essential role in the initiation and progression of cancer. In the present study, the role of a LncRNA, ARHGAP5-AS1 in breast cancer was investigated. Methods: Bioinformation was analyzed for the expression of ARHGAP5-AS1. qRT-PCR was conducted to verify the expression of ARHGAP5-AS1 in breast cancer specimens. Transwell migration assays and F-actin staining were utilized to estimate cell migration ability. RNA pulldown assays and RNA immunoprecipitation were used to prove the interaction between ARHGAP5-AS1 and SMAD7. Western blot and immunofluorescence imaging were used to examine the protein levels. Dual luciferase reporter assays were performed to evaluate the activation of TGF-β signaling.Results: Compared to MDA-MB-231 cells, the expression of LncRNA ARHGAP5-AS1(NR_027263) was significantly suppressed in its highly metastatic subtype MDA-MB-231-LM2 cells. Functional study showed ARHGAP5-AS1 could inhibit cell migration via suppression of stress fibers in breast cancer cell lines. Afterwards, SMAD7 was further identified to interact with ARHGAP5-AS1 by its PY motif and thus its ubiquitination and degradation was blocked due to reduced interaction with E3 ligase SMURF1 and SMURF2. Moreover, ARHGAP5-AS1 could inhibit TGF-β signaling pathway due to its inhibitory role on SMAD7. Conclusions: Overall, these findings demonstrate that ARHGAP5-AS1 inhibits breast cancer cell migration and could server as a novel biomarker for breast cancer metastasis and a potent target for the treatment in the future.
Value of multidetector computed tomography angiography before bronchial artery embolization in hemoptysis management and early recurrence prediction: a prospective study
Authors: Le Huu Y , Le Van Nam , Pham Ngoc Hung , Phung Anh Tuan , Nguyen Thanh Tung , Do Quyet 
Published: August 2020

Abstract
Background: Multidetector computed tomography (MDCT) angiography is an useful examination to detect the source of the bleeding in patients with hemoptysis. The aim of the study was to prospectively evaluate the role and clinical efficacy of MDCT angiography before bronchial artery embolization (BAE) for the management of hemoptysis, and to investigate the predictors of early recurrence.Methods: It is a double-center study which included 57 hemoptysis patients undergoing MDCT angiography prior to BAE from August 2019 to July 2020. A prospective analysis of culprit arteries detected by MDCT angiography allowed an evaluation of the role of this technique. A follow-up was done to assess the efficacy of BAE with preprocedural MDCT angiography and to explore the risk factors of early recurrent hemoptysis.Results: The accuracy of MDCT angiography in the identification of culprit arteries was as high as 97.5%. The average number of total culprit arteries per patient was 2.75 1.73. Among which, the average numbers of culprit ectopic bronchial arteries (BAs) and non-bronchial systemic arteries (NBSAs) per patient were 0.21 0.41 and 1.04 1.57, respectively. The immediate clinical success rate, total hemoptysis recurrence rate, and early hemoptysis recurrence rate of BAE following MDCT angiography were 94.7%, 18.5%, 16.7%, respectively. Aspergilloma (HR = 6.63, 95% CI: 1.31-33.60, p = 0.022) was associated with an increase in the risk of early recurrence.Conclusions: MDCT angiography should be performed before BAE for the management of hemoptysis. Aspergilloma was an independent predictor for early recurrence.
Identification of lncRNAs associated with the pathogenesis of ankylosing spondylitis
Authors: Huang Dan , Liu Jian , Wan Lei , Fang Yanyan , Long Yan , Zhang Ying , Bao Bingxi 
Published: August 2020

Abstract
Background
Ankylosing spondylitis (AS) is a chronic autoimmune disease affecting the sacroiliac joint. To date, few studies have examined the association between long non-coding RNAs (lncRNAs) and AS pathogenesis. As such, we herein sought to characterize patterns of AS-related lncRNA expression and to evaluate the potential role played by these lncRNAs in this complex autoimmune context.
Methods
We conducted an RNA-seq analysis of peripheral blood mononuclear cell samples isolated from five AS patients and corresponding controls. These data were then leveraged to characterize AS-related lncRNA expression patterns. We further conducted GO and KEGG enrichment analyses of the parental genes encoding these lncRNAs, and we confirmed the validity of our RNA-seq data by assessing the expression of six lncRNAs via qRT-PCR in 15 AS and control patient samples. Pearson correlation analyses were additionally employed to examine the associations between the expression levels of these six lncRNAs and patient clinical index values.
Results
We detected 56575 total lncRNAs in AS and control patient samples during our initial RNA-seq analysis, of which 200 and 70 were found to be up- and down-regulated (FC > 2 or < 0.05; P < 0.05), respectively, in AS samples relative to controls. In qRT-PCR validation assays, we confirmed the significant upregulation of NONHSAT118801.2, ENST00000444046, and NONHSAT183847.1 and the significant downregulation of NONHSAT205110.1, NONHSAT105444.2, and NONHSAT051856.2 in AS patient samples. We further found the expression of NONHSAT118801.2 and NONHSAT183847.1 to be positively correlated with disease severity.
Conclusion
Overall, our findings highlight several lncRNAs that are specifically expressed in the context of AS, indicating that they may play key functions in the pathogenesis of this autoimmune disease. Specifically, we determined that NONHSAT118801.2 and NONHSAT183847.1 may be valuable biomarkers of AS.
Implementation of Highly Challenging Balance Training for Parkinson’s disease in Clinical Practice: A Process Evaluation
Authors: Leavy Breiffni , Joseph Conran , Kwak Lydia , Franzén Erika 
Published: August 2020

Abstract
Background: Process evaluations provide contextual insight into the way in which interventions are delivered. This information is essential when designing strategies to implement programs into wider clinical practice. We performed a process evaluation of the HiBalance effectiveness trial investigating the effects of a 10-week of highly challenging and progressive balance training for mild-moderate Parkinson’s disease (PD). Study aims were to investigate i) the quality and quantity of intervention delivery and ii) barriers and facilitators for implementation.Methods: Process outcomes included; Fidelity; Dose (delivered and received) Recruitment and Reach. Investigation of barriers and facilitators was guided by the Consolidated Framework for Implementation Research. Program delivery was assessed across four neurological rehabilitation sites during a two-year period. Data collection was mixed-methods in nature and quantitative and qualitative data were merged during the analysis phase.Results: Thirteen program trainers delivered the intervention to 12 separate groups during 119 training sessions. Trainer fidelity to program core components was very high in 104/110 (94%) of the sessions. Participant responsiveness to the core components was generally high, although adherence to the home exercise program was low (50%). No significant context-specific differences were observed across sites in terms of fidelity, dose delivered/ received or participant characteristics, despite varying recruitment methods. Facilitators to program delivery were; PD-specificity, high training frequency and professional autonomy. Perceived barriers included; cognitive impairment, absent reactional balance among participants, as well a heterogeneous group in relation to balance capacity. Conclusion: These findings provide corroborating evidence for outcome evaluation results and valuable information for the further adaptation and implementation of this program. Important lessons can also be learned for researchers and clinicians planning to implement challenging exercise training programs for people with mild-moderate PD. Trial registration: ClinicalTrials.gov, NCT02727478, registered 30 march − Retrospectively registered. https://clinicaltrials.gov/ct2/show/NCT02727478
Bioinformatic analysis of the inner heterogeneity within MYCN non-amplified pediatric neuroblastoma sub-group
Authors: Wang Haiwei , Wang Xinrui , Xu Liangpu , Zhang Ji , Cao Hua 
Published: August 2020

Abstract
Background: Pediatric neuroblastoma is divided into MYCN amplified and MYCN non-amplified sub-groups. However, the extent of heterogeneity within MYCN amplified or non-amplified pediatric neuroblastoma is unclear.Methods: The prognostic significance of age and MYCN amplification was determined through multivariate cox regression and Kaplan-Meier survival analysis. MYCN non-amplified pediatric neuroblastoma patients were divided into different sub-consensuses using non-negative matrix factorization (NMF) based on the gene expression profiling. Genes particularly expressed in MYCN non-amplified younger neuroblastoma patients were identified using Therapeutically Applicable Research to Generate Effective Treatments (TARGET) and Gene Expression Omnibus (GEO) datasets. The prognostic effects of ALCAM, CACNA2D3, DST, EPB41L4A and KIFIB in MYCN non-amplified pediatric neuroblastoma patients were determined by Kaplan-Meier survival.Results: Age and MYCN amplification were independent prognostic factors in pediatric neuroblastoma. Compared with MYCN non-amplified older neuroblastoma patients, MYCN non-amplified younger neuroblastoma patients had better clinical outcomes. MYCN non-amplified pediatric neuroblastoma was divided into three sub-consensuses through NMF assay and each sub-consensus was with significantly different clinical outcomes. However, MYCN amplified pediatric neuroblastoma could not divide into sub-groups with different clinical outcomes by age or by NMF assay. ALCAM, CACNA2D3, DST, EPB41L4A and KIFIB were highly expressed in MYCN non-amplified younger neuroblastoma patients. Moreover, the high expression levels of ALCAM, CACNA2D3, DST, EPB41L4A or KIFIB were associated with the favorable prognosis of MYCN non-amplified neuroblastoma patients. We also found that DST was an independent prognostic factor in MYCN non-amplified neuroblastoma patients and MYCN non-amplified younger neuroblastoma patients with high DST expression levels had the best clinical overall survival.Conclusions: MYCN non-amplified neuroblastoma was a heterogeneous disease and could be divided into sub-groups based on age or the expression levels of ALCAM, CACNA2D3, DST, EPB41L4A or KIFIB. MYCN non-amplified younger neuroblastoma patients with high DST expression levels had the best clinical overall survival.
Cloning of Long Sterile Lemma (lsl2), A Single Recessive Gene Regulating Spike Germination in Rice (Oryza Sativa L.)
Authors: Yang Dewei , He Niqing , Zheng Xianghua , Zhen Yanmei , Xie Zhenxin , Cheng Chaoping , Huang Fenghuang 
Published: August 2020

Abstract
Background: Rice is a typical monocotyledonous plant and an important cereal crop. The structural units of rice flowers are spikelets and florets. Floral organ development and spike germination affect rice reproduction and yield. Results: In this study, we identified a novel long sterile lemma (lsl2) mutant from an EMS population. First, we mapped the lsl2 gene between the markers Indel7-22 and Indel7-27, which encompasses a region of 25 kb. The rice genome annotation indicates the presence of four candidate genes in this region. Through gene prediction and cDNA sequencing, we confirmed that the target gene in the lsl2 mutant is allelic to LONG STERILE LEMMA1 (G1)/ELONGATED EMPTY GLUME (ELE), hereafter referred to as lsl2. Further analysis showed a one-amino acid change, serine (Ser) 79 mutated to proline (Pro), in the lsl2 protein had compared with LSL2, which may change the function of the LSL2 protein. The knockout experiments showed that the lsl2 gene is responsible for the long sterile lemma phenotype. The lsl2 gene may reduce the damage caused by spike germination by decreasing the seed germination rate, and yet other agronomic traits of rice are not affected in the lsl2 mutant. Taken together, our results demonstrate that the lsl2 gene will have specific application prospects in future rice breeding.Conclusions: The lsl2 gene is responsible for the long sterile lemma phenotype, and may reduce the damage caused by spike germination by decreasing the seed germination rate.
Elucidating The Multifunctional Role of The Cell Wall Components in The Maize Exploitation
Authors: López-Malvar Ana , Malvar Rosa Ana , Souto Xose Carlos , Gomez Leonardo Dario , Simister Rachael , Encina Antonio , Barros-Rios Jaime , Pereira-Crespo Sonia , Santiago Rogelio 
Published: August 2020

Abstract
Background: Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used in silage production. However, yield is reduced by pest damages, where stem corn borers are one of the most important factors limiting yield. Overall, cell wall composition is key in determining the quality of maize biomass, as well as pest resistance. This study aims to address the interrelations between cell wall components in diverse maize genotypes, and pest resistance, ethanolic production and forage digestibility. Results: We can highlight the following results: (i) pests resistant inbred lines may show cell walls with low p-coumaric acid and hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and diferulate cross-linking may present higher performance in ethanol production; (iii) and inbred lines with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits. Conclusions: Our results evidence that there is no maize cell wall ideotype among the tested for optimal performance in the three areas evaluated, and maize plants should be specifically bred for each particular application.
The cost of breast cancer: A comparison between private and public hospitals
Authors: Afkar Abolhasan , Jalilian Habib , Pourreza Abolghasem , Mir Habibeh , Sigaroudi Abdolhosein Emami , Heydari Somayeh 
Published: August 2020

Abstract
Backgrounds Breast cancer is the most prevalent cancer among women. Breast cancer imposes a considerable economic burden on the health system. This study aimed to compare the cost of breast cancer among patients who referred to private and public hospitals in Iran (2017).Methods This was a prevalence-based cost of illness study. A total of 179 patients were selected from private and public hospitals using the census method. The researcher-constructed checklist was used for data collection. Data were analyzed using SPSS software version 22.Results The estimated total mean ± SD direct cost of patients who referred to the private hospital and the public hospital was $10051.78 ± 19484.61 and $3956.33 ± 6783.02, respectively. Further, the total mean indirect cost of patients who referred to the private hospital was lower than those referring to the public hospital at $1870.89 (%15.69 of total costs) and $22348.5 (%84.95 of total costs), respectively. These differences were statistically significant (P < 0.05).Conclusion Breast cancer imposes a substantial cost on patients, health insurance organizations and the whole society in Iran. Therefore, the adoption of effective measures for the prevention and early diagnosis of breast cancer is urgently needed.
Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells
Authors: Liu Dahai , Hui Yubo , Wang Junrong , Ye Cong , Du Jianshi 
Published: August 2020

Abstract
BackgroundThe hyperproliferation of mesoblastic vascular tissues can lead to the incidence of hemangioma (IHA), which is the most common benign tumor in infants. Estrogenic signals can trigger the progression of HA via activation of gene transcription.ResultsWe found that bisphenol S (BPS), one widely spread endocrine disrupting compound (EDC), can induce the proliferation of HA cells and trigger the G1 to S transition of cell cycle. Among the tested cytokines, BPS can up regulate of basic fibroblast growth factor (bFGF). Targeted inhibition of bFGF via its neutralization antibody can reverse BPS induced cell proliferation. Mechanistically, BPS can increase the mRNA expression of bFGF via increasing the transcription and mRNA stability. The activation of p65 and down regulation of miR-155-5p were responsible for BPS induced transcription and mRNA stability of bFGF, respectively. Conclusions: BPS can increase the expression of bFGF via activation of p65 and down regulation of miR-155-5p, which resulted in the proliferation of HA cells.
Application of Wire-Assisted Reduction Guided by Minimally Invasive Wire Introducer in the Treatment of Irreducible Femoral Intertrochanteric Fractures
Authors: Lin Xiaocong , Huang Xiuxi , Fang Kaibin , Ke Qingfeng , Shi Shaojian , Dai Zhangsheng 
Published: August 2020

Abstract
Background: Open reduction was often required in the treatment of irreducible femoral intertrochanteric fractures. A minimally invasive wire introducer was designed to assist the reduction of such fractures. The aim of our study was to investigate the clinical outcomes of this technique.Methods: Between 2013 and 2018, 92 femoral intertrochanteric fracture patients who were treated with intramedullary nail fixation and difficult reduction using the traction beds were retrospectively reviewed. Decision for surgery was based on the displacement of the fracture. The patients was divided into two groups, 31 in the control group and 61 in the observation group. The control group were received open reduction operation. And the observation group still received the closed reduction operation, using minimally invasive wire introducer to guide the wire and assist the fracture reduction. The operation time, blood loss, VAS scores, angulation, reduction, neck-shaft angle, redisplacement, limb length discrepancy, and union time were recorded.Results: All patients of two groups were successfully operated and were followed up for an average of 23.8 months. There was no statistical difference in baseline data between the two groups. The observation group had shorter operation time, lower VAS score and less intraoperative bleeding. And the datas are statistically different.Conclusion: Minimally invasive wire introducer is a good technology to guide the wire for irreduciblr femoral intertrochanteric fracture reduction. Using this technique, irreduciblr femoral intertrochanteric fractures could be restored and good clinical outcome was achieved.Level of evidence: Level IV; Case Series; Treatment Study