bluuebunny/crossref_metadata_2025_split huggingface dataset:
The journey to engaged customer community: Evidential social CRM maturity model in Twitter
Authors: Yahav Inbal , Schwartz David G. , Welcman Yaara 
Published: December 2019

AbstractWe investigate the company use of Twitter as a platform for social customer relations management (SCRM) to find that message type and follower growth follow an identifiable maturity model. Studying longitudinal Twitter data from 73 Standard and Poor's companies, we find that companies map into one of two distinct maturity stages as reflected in the content of company‐generated messages. The first maturity stage consists of branding, where the focus is on customer acquisition up to a certain volume, at the expense of their engagement. The second is social care, where the focus is on retaining customers and increased engagement. We further find that companies have relatively little control over their maturity stage and that investing in social care in the first stage, aiming to increase engagement, is not effective. Proper understanding and recognition of the Twitter SCRM maturity model will allow companies to assess their methods and processes according to SCRM best practices.
Telomere‐Specialized Retroelements in Drosophila: Adaptive Symbionts of the Genome, Neutral, or in Conflict?
Authors: Markova Dragomira N. , Christensen Shawn M. , Betrán Esther 
Published: December 2019

AbstractLinear chromosomes shorten in every round of replication. In Drosophila, telomere‐specialized long interspersed retrotransposable elements (LINEs) belonging to the jockey clade offset this shortening by forming head‐to‐tail arrays at Drosophila telomere ends. As such, these telomeric LINEs have been considered adaptive symbionts of the genome, protecting it from premature decay, particularly as Drosophila lacks a conventional telomerase holoenzyme. However, as reviewed here, recent work reveals a high degree of variation and turnover in the telomere‐specialized LINE lineages across Drosophila. There appears to be no absolute requirement for LINE activity to maintain telomeres in flies, hence the suggestion that the telomere‐specialized LINEs may instead be neutral or in conflict with the host, rather than adaptive.
Oxidative stress‐alleviating strategies to improve recombinant protein production in CHO cells
Authors: Chevallier Valentine , Andersen Mikael Rørdam , Malphettes Laetitia 
Published: December 2019

AbstractLarge scale biopharmaceutical production of biologics relies on the overexpression of foreign proteins by cells cultivated in stirred tank bioreactors. It is well recognized and documented fact that protein overexpression may impact host cell metabolism and that factors associated with large scale culture, such as the hydrodynamic forces and inhomogeneities within the bioreactors, may promote cellular stress. The metabolic adaptations required to support the high‐level expression of recombinant proteins include increased energy production and improved secretory capacity, which, in turn, can lead to a rise of reactive oxygen species (ROS) generated through the respiration metabolism and the interaction with media components. Oxidative stress is defined as the imbalance between the production of free radicals and the antioxidant response within the cells. Accumulation of intracellular ROS can interfere with the cellular activities and exert cytotoxic effects via the alternation of cellular components. In this context, strategies aiming to alleviate oxidative stress generated during the culture have been developed to improve cell growth, productivity, and reduce product microheterogeneity. In this review, we present a summary of the different approaches used to decrease the oxidative stress in Chinese hamster ovary cells and highlight media development and cell engineering as the main pathways through which ROS levels may be kept under control.
Regulation of apoptosis by E3 ubiquitin ligases in ubiquitin proteasome system
Authors: Sharma Akshay , Trivedi Arun K. 
Published: December 2019

AbstractApoptosis is an organised ATP‐dependent programmed cell death that organisms have evolved to maintain homoeostatic cell numbers and eliminate unnecessary or unhealthy cells from the system. Dysregulation of apoptosis can have serious manifestations culminating into various diseases, especially cancer. Accurate control of apoptosis requires regulation of a wide range of growth enhancing as well as anti‐oncogenic factors. Appropriate regulation of magnitude and temporal expression of key proteins is vital to maintain functional apoptotic signalling. Controlled protein turnover is thus critical to the unhindered operation of the apoptotic machinery, disruption of which can have severe consequences, foremost being oncogenic transformation of cells. The ubiquitin proteasome system (UPS) is one such major cellular pathway that maintains homoeostatic protein levels. Recent studies have found interesting links between these two fundamental cellular processes, wherein UPS depending on the cue can either inhibit or promote apoptosis. A diverse range of E3 ligases are involved in regulating the turnover of key proteins of the apoptotic pathway. This review summarises an overview of key E3 ubiquitin ligases involved in the regulation of the fundamental proteins involved in apoptosis, linking UPS to apoptosis and attempts to emphasize the significance of this relationship in context of cancer.
Intermetallic Compounds as an Alternative to Single‐atom Alloy Catalysts: Geometric and Electronic Structures from Advanced X‐ray Spectroscopies and Computational Studies
Authors: Wegener Evan C. , Bukowski Brandon C. , Yang Dali , Wu Zhenwei , Kropf A. Jeremy , Delgass W. N. , Greeley Jeffrey , Zhang Guanghui , Miller Jeffrey T. 
Published: December 2019

AbstractPt−Fe intermetallic compound (IMC) catalysts, including Pt3Fe, PtFe and PtFe3, are shown to have advantageous catalytic properties similar to those reported for single‐atom alloy catalysts. Suppresion of the Pt ensembles responsible for hydrogenolysis results in high olefin selectivity during propane dehydrogenation. In situ resonant inelastic X‐ray scattering (RIXS) results and density functional theory calculations show that these changes are associated with a decrease in the average energy of the filled 5d states of Pt in the Pt−Fe intermetallic compound structures compared to monometallic Pt, accompanied by an increase in the average energy of the unfilled valence bands. The decrease in energy of the filled Pt 5d orbitals increases with increasing Fe content in the IMC, i.e. PtFe3>PtFe>Pt3Fe. These results demonstrate that by altering the stoichiometry of IMC catalysts it is possible to control both the ensemble size and electronic properties of active sites, which affords another mechanism for tuning catalytic properties, in addition to changing promoter metals. The present study demonstrates the potential of ordered intermetallic compounds as an alternative to traditional solid‐solution single‐atom alloys to serve as catalysts with well‐defined and uniform active sites.
Comparative Study of the Flue Gas Desulfurization Using Different‐Grade Natural Manganese Oxides
Authors: Chen Yongxiang , Li Jianguo , Li Yunjiao , Cao Xinlong , Tang Sanchuan , Wang Shilei , Chang Shenhong , Ye Wanqi , Li Wei 
Published: December 2019

AbstractDifferent grades of natural manganese ores were used to remove SO2 by dry flue gas desulfurization. The grades of the manganese ores were negatively correlated with the desulfurization performance. The produced MnSO4 enriched on the surface would block the gas diffusion channels during desulfurization. The better desulfurization properties of the low‐grade manganese ores are ascribed to the highly active γ‐MnO2, the porous structure, and the high specific surface area as well as the SiO2 bridge‐like effects. This study supports the cooperative use of sulfur‐containing flue gases and low‐grade natural manganese oxides.
Modeling of Mixing‐Precipitation Processes: Agglomeration
Authors: Orlewski Pawel M. , Mazzotti Marco 
Published: December 2019

AbstractA comprehensive description of the barium sulfate precipitation process in a wide range of supersaturations is presented. By using an additive to stabilize the particles, the decoupling of the primary from the secondary processes, as well as the agglomeration from aggregation was possible. By being able to study the two processes independently, a model describing the agglomeration of barium sulfate in the range of high supersaturations was validated experimentally for the first time. The proposed model has proven to describe the experiments with a high degree of accuracy in the whole range of supersaturations investigated. Additionally, by comparing agglomeration kernels of various complexity, ranges where simplifications are possible were identified, thus enabling the future development of models with better performance.
Effect of Electrolyte Ions on the Formation, Electroactivity, and Rectification Properties of Films Obtained by Electrografting
Authors: Ramírez‐Chan Daniel E. , Fragoso‐Soriano Rogelio , González Felipe J. 
Published: December 2019

AbstractThe oxidation of 4‐(4‐nitrophenyl)butyrate ions in acetonitrile was used to modify glassy carbon surfaces with films bearing 3‐(4‐nitrophenyl)propyl groups. The main features of the voltammetric reduction of the grafted nitrophenyl groups were studied in two different supporting electrolytes; n‐Bu4NPF6 and Me4NPF6. The best response was obtained with the less bulky electrolyte, which was explained by the major capability of these ions to diffuse inside the channels of the grafted film, as well as the better stabilization of the radical anions generated during the reduction process. It was also shown that the films electrografted in presence of the bulky n‐Bu4NPF6 are thicker but less compact than those prepared in Me4NPF6. The amount of nitrophenyl groups in the grafted film that can be reduced depends on the size of the electrolyte ions. The permeation of the electrolyte ions inside the film channels determines also the voltammetric behavior of ferrocene as redox probe. A current rectification phenomenon was observed in acetonitrile solutions of bulky and hydrophobic electrolytes like n‐Bu4NPF6 and n‐Hx4NPF6, whose marginal inclusion inside the film channels promotes both the entrance of ferrocene and the expelling of ferrocenium ions from these ones. This phenomenon was emulated through a CEC mechanism in solution, where the diffusion inside the channels was described as single chemical equilibria.
Intermetallic PtBi Nanoplates with High Catalytic Activity towards Electro‐oxidation of Formic Acid and Glycerol
Authors: Wang Chang‐Yi , Yu Zhi‐Yuan , Li Gen , Song Qian‐Tong , Li Guang , Luo Chen‐Xu , Yin Shu‐Hu , Lu Bang‐An , Xiao Chi , Xu Bin‐Bin , Zhou Zhi‐You , Tian Na , Sun Shi‐Gang 
Published: December 2019

AbstractDespite considerable progress made for direct liquid fuel cells, developing efficient electrocatalysts for the oxidation of small organic molecules reaction remains a challenge. Herein, we employed a facile one‐pot method to synthesize hexagonal intermetallic PtBi nanoplates. Benefiting from the unique structure and third body effect, the mass activity of formic acid oxidation on PtBi/C at peak potential reaches 9.06 A ⋅ mgPt−1, which is 10.4 times higher than that of commercial Pt/C catalyst, making it the most efficient electrocatalyst ever reported. In situ FTIR spectroscopic studies revealed that the introduction of Bi suppresses dramatically CO‐poisoning, which effectively improves the activity and stability towards formic acid oxidation. Furthermore, in situ FTIR spectra of glycerol oxidation indicate that much more oxalate and tartronate are produced on PtBi/C than that on Pt/C, and a plausible reaction mechanism for the oxidation of glycerol on PtBi/C catalyst in alkaline solution was proposed.
Electrocatalytic Hydrogen Production with a Molecular Cobalt Complex in Aqueous Solution
Authors: Wilken Mona , Siewert Inke 
Published: December 2019

AbstractHerein, we present the synthesis of a cobalt complex with a pentadentate ligand platform and its application in the electrochemical hydrogen evolution reaction (LCF3=2,6‐bis(methoxybis(4‐trifluormethyl‐1H‐imidazol‐2‐yl)methyl)pyridine). The complex is active in neutral to acidic solvent mixtures containing 50 % water. Electronic tuning in the ligand backbone, that is introduction of electron‐withdrawing CF3‐groups, shifts the onset potential of the catalytic wave to less negative potentials. NMR and UV/Vis studies indicate that the CoIII complex is deprotonated twice in the ligand backbone under neutral conditions. Reduction is accompanied by protonation, as indicated by pH‐dependent CV measurements, and the CoI state initiates catalysis. The result suggests that the pentadentate ligand is an ideal platform for cobalt catalysts in the oxidative and reductive water splitting reaction.
Photoinduced, Direct C(sp2)−H Bond Azo Coupling of Imidazoheteroarenes and Imidazoanilines with Aryl Diazonium Salts Catalyzed by Eosin Y
Authors: Saba Sumbal , Dos Santos Caio R. , Zavarise Bruno R. , Naujorks Aline A. S. , Franco Marcelo S. , Schneider Alex R. , Scheide Marcos R. , Affeldt Ricardo F. , Rafique Jamal , Braga Antonio L. 
Published: December 2019

AbstractHerein, a greener approach to the eosin Y‐Na2 catalyzed, C(sp2)−H bond azo coupling of imidazoheteroarene with aryl diazonium salts is described, under acid free conditions. This direct photoredox process resulted in the corresponding azo products in good to excellent yields. Besides, this new approach could also be applicable to anilines, which is a poorly reactive substrate by other methods. The main features of this reaction are that it provides high yields and is gram‐scalable and applicable to biologically relevant imidazoheteroarenes and ‐anilines.
An algorithm for coupling multibranch in vitro experiment to numerical physiology simulation for a hybrid cardiovascular model
Authors: Mirzaei Ehsan , Farahmand Masoud , Kung Ethan 
Published: December 2019

AbstractThe hybrid cardiovascular modeling approach integrates an in vitro experiment with a computational lumped‐parameter simulation, enabling direct physical testing of medical devices in the context of closed‐loop physiology. The interface between the in vitro and computational domains is essential for properly capturing the dynamic interactions of the two. To this end, we developed an iterative algorithm capable of coupling an in vitro experiment containing multiple branches to a lumped‐parameter physiology simulation. This algorithm identifies the unique flow waveform solution for each branch of the experiment using an iterative Broyden's approach. For the purpose of algorithm testing, we first used mathematical surrogates to represent the in vitro experiments and demonstrated five scenarios where the in vitro surrogates are coupled to the computational physiology of a Fontan patient. This testing approach allows validation of the coupling result accuracy as the mathematical surrogates can be directly integrated into the computational simulation to obtain the “true solution” of the coupled system. Our algorithm successfully identified the solution flow waveforms in all test scenarios with results matching the true solutions with high accuracy. In all test cases, the number of iterations to achieve the desired convergence criteria was less than 130. To emulate realistic in vitro experiments in which noise contaminates the measurements, we perturbed the surrogate models by adding random noise. The convergence tolerance achievable with the coupling algorithm remained below the magnitudes of the added noise in all cases. Finally, we used this algorithm to couple a physical experiment to the computational physiology model to demonstrate its real‐world applicability.
Superior Electrochemical Performance of Pristine Nickel Hexaaminobenzene MOF Supercapacitors Fabricated by Electrophoretic Deposition
Authors: Wechsler S. C. , Amir F. Z. 
Published: December 2019

Abstract2 D Metal–organic frameworks (MOFs) are promising materials for supercapacitor electrodes because of their controllable structure, tunable pore size, and high specific surface area. In this study, the fabrication of pristine MOF nickel hexaaminobenzene Ni3(HAB)2 supercapacitor electrodes by electrophoretic deposition (EPD) was reported. The MOF‐based symmetric supercapacitor demonstrated a superior electrochemical capacitive performance over a potential window of 0–1.0 V and displayed an areal capacitance of 13.64 mF cm−2 and a remarkable ultra‐high cycling stability with a retention of 81 % over 50 000 cycles. The supercapacitor's outstanding performance was attributed to the binder‐free EPD process and to the 2 D MOF nanosheets, which facilitate ion diffusion throughout the electrodes. These promising results demonstrate the potential of using pristine MOFs as the next generation of materials for energy‐storage applications.
Natural Polymers as Green Binders for High‐Loading Supercapacitor Electrodes
Authors: Ruschhaupt Peter , Varzi Alberto , Passerini Stefano 
Published: December 2019

AbstractThe state‐of‐the‐art aqueous binder for supercapacitors is carboxymethyl cellulose (CMC). However, it limits the mass loading of the coatings owing to shrinkage upon drying. In this work, natural polymers, that is, guar gum (GG), wheat starch (WS), and potato starch (PS), were studied as alternatives. The flexibility and adhesion of the resulting coatings and electrochemical performance was tested. The combination of 75:25 (w/w) ratio PS/GG showed a promising performance. Electrodes were characterized by SEM, thermal, adhesion, and bending tests. Their electrochemical properties were determined by cyclic voltammetry, electrochemical impedance spectroscopy, and cycling experiments. The PS/GG mixture conformed well to criteria for industrial production, enabling mass loadings higher than CMC (7.0 mg cm−2) while granting the same specific capacitance (26 F g−1) and power performance (20 F g−1 at 10 A g−1). Including the mass of the current collector, this represents a +45 % increase in specific energy at the electrode level.
Flow cytometry aneuploidy and cell cycle indexing as a possible tool for differentiating between CD10+ diffuse large B‐cell lymphoma and follicular lymphoma
Authors: Azoulay David , Cohen Hector I. , Dementiev Eugene , Eshel Elizabeth , Akria Luiza , Shaoul Ety , Horowitz Netanel 
Published: December 2019

AbstractBackgroundDifferential diagnosis between diffuse large B‐cell lymphoma (DLBCL) and follicular lymphoma (FL) becomes a challenge when adequate biopsy is unavailable. The present study aimed to investigate the applicability of DNA cell cycle analysis by flow cytometry (FC) for differentiating between CD10+ DLBCL and FL.MethodsData were collected from 57 specimens where CD5−/CD10+/light chain restricted B cells were detected. DNA staining was performed using the Coulter DNA Prep Kit. Cell cycle fractions were evaluated by automatic analysis using the ModFit LT software.ResultsHistopathological analysis confirmed the diagnosis of CD10+ FL in 30 specimens (52.6%), CD10+ DLBCL in 24 specimens (42.1%), and CD10+ Burkitt lymphoma in 3 specimens (5.3%). A significantly higher rate of DNA aneuploidy was detected among CD10+ DLBCL than FL specimens (50 vs. 13.3% respectively, p = .003). Likewise, DNA index was significantly higher in CD10+ DLBCL relative to FL (1.26 ± 0.35 vs. 1.04 ± 0.16 respectively, p = .004). Notably, the proportion of cells in the S‐phase and proliferative fraction was significantly higher in CD10+ DLBCL than in CD10+ FL (S‐phase: 15.97 ± 13.94 vs. 4.43 ± 4.41 mean ± SD, respectively, p < .0001; proliferative fraction: 18.87 ± 15.17 vs. 5.78 ± 7.04 mean ± SD, respectively, p = .0001). Using a receiver operating characteristic analysis, optimal cutoffs for S‐phase ≥7% and proliferative fraction ≥9% were determined. These values could be used to differentiate between CD10+ DLBCL and CD10+ FL.ConclusionIncluding DNA cell cycle analysis in the FC lymphoma assessment panel may be of diagnostic value in differentiating between CD10+ DLBCL and FL when adequate biopsy is unavailable.
Linking community assembly and structure across scales in a wild mouse parasite community
Authors: Rynkiewicz Evelyn C. , Fenton Andy , Pedersen Amy B. 
Published: December 2019

AbstractUnderstanding what processes drive community structure is fundamental to ecology. Many wild animals are simultaneously infected by multiple parasite species, so host–parasite communities can be valuable tools for investigating connections between community structures at multiple scales, as each host can be considered a replicate parasite community. Like free‐living communities, within‐host–parasite communities are hierarchical; ecological interactions between hosts and parasites can occur at multiple scales (e.g., host community, host population, parasite community within the host), therefore, both extrinsic and intrinsic processes can determine parasite community structure. We combine analyses of community structure and assembly at both the host population and individual scales using extensive datasets on wild wood mice (Apodemus sylvaticus) and their parasite community. An analysis of parasite community nestedness at the host population scale provided predictions about the order of infection at the individual scale, which were then tested using parasite community assembly data from individual hosts from the same populations. Nestedness analyses revealed parasite communities were significantly more structured than random. However, observed nestedness did not differ from null models in which parasite species abundance was kept constant. We did not find consistency between observed community structure at the host population scale and within‐host order of infection. Multi‐state Markov models of parasite community assembly showed that a host's likelihood of infection with one parasite did not consistently follow previous infection by a different parasite species, suggesting there is not a deterministic order of infection among the species we investigated in wild wood mice. Our results demonstrate that patterns at one scale (i.e., host population) do not reliably predict processes at another scale (i.e., individual host), and that neutral or stochastic processes may be driving the patterns of nestedness observed in these communities. We suggest that experimental approaches that manipulate parasite communities are needed to better link processes at multiple ecological scales.