bluuebunny/crossref_metadata_2025_split huggingface dataset:
The German Corona Consensus Dataset (GECCO): A standardized dataset for COVID-19 research
Authors: Thun Sylvia , Sass Julian , Bartschke Alexander , Lehne Moritz , Essenwanger Andrea , Rinaldi Eugenia , Rudolph Stefanie , Heitmann Kai U. , Vehreschild Jörg J. , von Kalle Christof 
Published: August 2020

Abstract
Background: The current COVID-19 pandemic has led to a surge of research activity. While this research provides important insights, the multitude of studies results in an increasing segmentation of information. To ensure comparability across projects and institutions, standard datasets are needed. Here, we introduce the “German Corona Consensus Dataset” (GECCO), a uniform dataset that uses international terminologies and health IT standards to improve interoperability of COVID-19 data.Methods: Based on previous work (e.g., the ISARIC-WHO COVID-19 case report form) and in coordination with experts from university hospitals, professional associations and research initiatives, data elements relevant for COVID-19 research were collected, prioritized and consolidated into a compact core dataset. The dataset was mapped to international terminologies, and the Fast Healthcare Interoperability Resources (FHIR) standard was used to define interoperable, machine-readable data formats.Results: A core dataset consisting of 81 data elements with 281 response options was defined, including information about, for example, demography, anamnesis, symptoms, therapy, medications or laboratory values of COVID-19 patients. Data elements and response options were mapped to SNOMED CT, LOINC, UCUM, ICD-10-GM and ATC, and FHIR profiles for interoperable data exchange were defined.Conclusion: GECCO provides a compact, interoperable dataset that can help to make COVID-19 research data more comparable across studies and institutions. The dataset will be further refined in the future by adding domain-specific extension modules for more specialized use cases.
Paternal and Maternal Support on Children's Weekday and Weekend Moderate-to-vigorous Physical Activity: a Cross-sectional Study
Authors: An Meijing , Chen Tianjiao , Zhou Qianling , Ma Jun 
Published: August 2020

Abstract
Background: Most studies about parental support behaviors for physical activity (PA) on children’s moderate-to-vigorous intensity physical activity (MVPA) were conducted in developed countries, and most of them focused on mother’s or parent’s (with no differentiation between father’s or mother’s) support behaviors. Besides, children’s MVPA time interval was not differentiated adequately. This paper aimed at investigating the associations between paternal and maternal support behaviors for PA, and children’s MVPA on weekdays, weekends and the whole week in China.Methods: Cross-sectional data of 517 father-child dyads and 1,422 mother-child dyads were analyzed. Children’s consecutive 7-day PA diary were recorded to assess their MVPA time, and MVPA time on weekdays, weekends and the whole week were further calculated, respectively. Father or mother completed a questionnaire on their support behaviors for children’s PA. Multivariate logistic regression was conducted to investigate the independent effect of paternal and maternal support behaviors for PA on children’s meeting MVPA recommendation on weekdays, weekends and the whole week, respectively.Results: The proportion of children’s meeting MVPA recommendation on weekends (37.8%) was significantly lower than that on weekdays (62.8%). Higher paternal (OR[95%CI]:1.098[1.009,1.195]) and maternal (OR[95%CI]:1.076[1.021,1.134]) total scores of support behaviors for PA were associated with children’s higher odds of meeting MVPA recommendation on weekends, after controlling for covariates. Paternal sharing PA knowledge with child was significantly associated with children’s meeting MVPA recommendation on weekends (OR[95%CI]:1.319[1.055,1.649]), and marginally associated with children’s meeting MVPA recommendation on weekdays (OR[95%CI]:1.220[0.974,1.528], P=0.084) and on the whole week (OR[95%CI]:1.218[0.977,1.519], P=0.080). Maternal reserving PA time for child was associated with children’s higher likelihood of meeting MVPA recommendation on weekdays (OR[95%CI]:1.160[1.025,1.313]), weekends (OR[95%CI]:1.241[1.097,1.403]) and the whole week (OR[95%CI]:1.214[1.076,1.369]), respectively.Conclusions: On weekends, paternal and maternal total support behaviors for PA deserves more practices for promoting children’s MVPA. To promote children’s MVPA on weekdays, weekends and the whole week, father’s sharing PA knowledge with child and mother’s reserving PA time for child are recommended. Longitudinal researches are needed to verify the effect of paternal and maternal support behaviors on children’s MVPA.
Association Between Caregivers’ Knowledge and Perceptions and Whether their Children Complete Tuberculosis Preventive Treatment
Authors: Zeladita Jhon Alex , Yuen Courtney M. , Zegarra-Chapoñan Roberto , Curisinche-Rojas Maricela , Egusquiza-Pozo Vicente 
Published: August 2020

Abstract
Background: Giving preventive therapy to children living in the households of TB patients to prevent them from developing TB themselves is important for reducing morbidity and mortality in this high-risk group. Children’s adherence to medications is likely dependent on their caregivers. We conducted a study to assess the association between caregivers’ knowledge and perceptions around isoniazid preventive therapy (IPT) and whether their children completed IPT. Methods: We conducted a retrospective medical record review of children who initiated IPT during 2017-2018 in 50 health facilities in south Lima, Peru. We administered structured surveys to caregivers of the children and about their knowledge and perceptions around IPT. To determine factors associated with IPT completion, we used a modified Poisson regression with generalized estimating equations to account for clustering by household. Results: We included in the study 550 children, of whom 31% did not complete IPT. Independent factors associated with not completing IPT were: low caregiver knowledge about TB and IPT (adjusted risk ratio [aRR]: 1.41; 95% confidence interval [CI]: 1.06-1.78), low caregiver perception of the importance of IPT (aRR: 1.76; 95% CI: 1.30-2.39), low caregiver satisfaction with the health services (aRR: 1.57; 95% CI: 1.14-2.16), experiencing adverse events (aRR: 2.08; 95% CI: 1.51-2.87), and living in a household with moderate or severe family dysfunction (aRR: 1.53; 95% CI: 1.07-2.19). Conclusions: IPT completion among children was associated with the knowledge and perceptions of their caregivers, as well as whether they experienced adverse events. To improve IPT completion among children, health care providers should prioritize education and counseling for caregivers, promote positive interpersonal relationships with them, and monitor adverse events.
Altered Striatal Dopamine Levels in Parkinson’s Disease VPS35 D620N Mutant Transgenic Aged Mice
Authors: Vanan Sarivin , Zeng Xiaoxia , Chia Sook Yoong , Varnäs Katarina , Ke Zhang , Mei Jiang , Padmanabhan Parasuraman , Yu Wei-Ping , Zhou Zhi-Dong , Halldin Christer , Gulyás Balázs , Tan Eng-King , Li Zeng 
Published: August 2020

Abstract
Vacuolar protein sorting 35 (VPS35) is a major component of the retromer complex that mediates the retrograde transport of cargo proteins from endosomes to the trans-Golgi network. Mutations such as D620N in the VPS35 gene have been identified in patients with autosomal dominant Parkinson’s disease (PD). However, it remains poorly understood whether and how VPS35 deficiency or mutation contributes to PD pathogenesis; specifically, the studies that have examined VPS35 thus far have differed in results and methodologies. We generated a VPS35 D620N mouse model using a Rosa26-based transgene expression platform to allow expression in a spatial manner, so as to better address these discrepancies. Here, aged (20-months-old) mice were first subjected to behavioral tests. Subsequently, DAB staining analysis of substantia nigra (SN) dopaminergic neurons with the marker for tyrosine hydroxylase (TH) was performed. Next, HPLC was used to determine dopamine levels, along with levels of its two metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), in the striatum. Western blotting was also performed to study the levels of key proteins associated with PD. Lastly, autoradiography (ARG) evaluation of [3H]FE-PE2I binding to the striatal dopamine transporter DAT was carried out. We found that VPS35 D620N Tg mice displayed a significantly higher dopamine level than NTg counterparts. All results were then compared with that of current VPS35 studies to shed light on the disease pathogenesis. Our model allows future studies to explicitly control spatial expression of the transgene which would generate a more reliable PD phenotype.
Consolidated Bioprocessing of Cellulose to Itaconic Acid by a Co-Culture of Trichoderma Reesei and Ustilago Maydis.
Authors: Schlembach Ivan , Tehrani Hamed , Blank Lars Mathias , Büchs Jochen , Wierckx Nick , Regestein Lars , Rosenbaum Miriam 
Published: August 2020

Abstract
Background: Itaconic acid is a bio-derived platform chemical with uses ranging from polymer synthesis to biofuel production. The efficient conversion of cellulosic waste streams into itaconic acid could thus enable the sustainable production of a variety of substitutes for fossil oil based products. However, the realization of such a process is currently hindered by an expensive conversion of cellulose into fermentable sugars. Here, we present the stepwise development of a fully consolidated bioprocess (CBP), which is capable to directly convert recalcitrant cellulose into itaconic acid without the need for separate cellulose hydrolysis including the application of commercial cellulases. The process is based on a synthetic microbial consortium of the cellulase producer Trichoderma reesei and the itaconic acid producing yeast Ustilago maydis. Results: The efficiency of the process was compared to a simultaneous hydrolysis and fermentation setup (SSF). Because of the additional substrate consumption of T. reesei in the CBP, the itaconic acid yield was significantly lower in the CBP than in the SSF. In order to increase yield and productivity of itaconic acid in the CBP, the population dynamics was manipulated by varying the inoculation delay between T. reesei and U. maydis. Surprisingly, neither inoculation delay nor inoculation density significantly affected the population development or the CBP performance. Instead, the substrate availability was the most important parameter. U. maydis was only able to grow and to produce itaconic acid when the cellulose concentration and thus, the sugar supply rate, was high. Finally, the metabolic processes during fed-batch CBP were analyzed in depth by online respiration measurements. A method was developed to estimate cellulose consumption, itaconic acid formation as well as the actual itaconic acid production yield online. Again, substrate availability was the key factor also controlling itaconic acid yield. In summary, an itaconic acid titer of 34 g/L with a total productivity of up to 0.07 g/L/h and a yield of 0.16 g/g could be reached during fed-batch cultivation. Conclusion: This study demonstrates the feasibility of consortium based CBP for itaconic acid production and also lays the fundament for the development and improvement of similar microbial consortia for cellulose based organic acid production.
Assessing the evidence on the differential impact of menthol versus non-menthol cigarette use on smoking cessation: A systematic review
Authors: Kim Mimi , Curtin Geoffrey 
Published: August 2020

Abstract
Background: This systematic review followed PRISMA guidelines to examine the Key Question: Does menthol cigarette use have a differential impact on smoking cessation compared with non-menthol cigarette use?Methods: The original protocol was registered on March 22, 2016 (updated January 10, 2019; PROSPERO: CRD42019119301). Six databases were queried from inception to December 14, 2018.Results: Fifty-seven studies (27 rated “good”, 27 rated as “fair”, and three studies rated as “poor” individual study quality) that compared menthol and non-menthol smokers were qualitatively synthesized across the following cessation measures (total adjusted studies; strength of evidence grade): duration of abstinence (2; low); quit attempts (14; insufficient); rate of abstinence/quitting (28; moderate); change in smoking quantity/frequency (3; insufficient); and return to smoking/relapse (2; insufficient). Overall, the qualitative synthesis failed to show a consistent trend for the association of menthol cigarette use and smoking cessation across the outcomes. Further, meta-analytic results found no difference between menthol and non-menthol cigarette use and the two measures of quit attempts and duration of abstinence.Implications: The overall strength of evidence for an association between menthol cigarette use and smoking cessation was graded as “low”, based on deficiencies of indirectness and inconsistency in the available body of evidence. Therefore, there is no consistent, significant, or differential association between menthol cigarette use and smoking cessation.
Alectinib Treatment Improves Photodynamic Therapy in Cancer Cell Lines of Different Origin
Authors: Gillissen Bernhard , Richter Antje , Kemmner Wolfgang 
Published: August 2020

Abstract
Background: Photodynamic therapy with a photosensitizer such as protoporphyrin-IX, a light sensitive metabolite of heme synthesis, is a highly selective treatment for various carcinomas. In previous studies, we found a significant down regulation of the relevant enzyme ferrochelatase in gastrointestinal carcinomas leading to an accumulation of protoporphyrin-IX within the tumor cells. Recent studies showed that a novel anti-cancer drug, Alectinib, an orally available, highly selective, potent second-generation inhibitor of anaplastic lymphoma tyrosinkinase binds to ferrochelatase. Therefore, we were interested to see whether Alectinib treatment might lead to an accumulation of protoporphyrin IX. Methods : Tumor cells of different origin were cultured, treated with LED-light and Alectinib. Results were gained by flow cytochemistry, immunohistochemistry and western blotting. Apoptosis was determined by flow cytometric analysis of Annexin V-FITC stained cells. In addition, cells were counterstained with propidium iodide (PI) to distinguish early apoptotic cells (Annexin V-positive/PI-negative) and late apoptosis/necroptotic cells.Results: Here, we report that photodynamic treatment of tumor cell lines of different origin in combination with Alectinib increased protoporphyrin-IX specific fluorescence and concomitantly cell death. Conclusions: The usage of Alectinib might be a crucial step for enhancing the effectiveness of photodynamic therapy. Further experiments will show whether photodynamic therapy in combination with Alectinib could be a new strategy for the treatment of e.g. peritoneal disseminated carcinomas.
Relationship between the Severity of Endometriosis Symptoms (Dyspareunia, Dysmenorrhea and Chronic pelvic pain) with the Spread of the Disease on Ultrasound
Authors: Kor Elham , Mostafavi Seyed Reza Saadat , Mazhin Zahra Ahmadian , Dadkhah Adeleh , Kor Anis , Habibi Shirin , Noroozi Shima Ghafourian , Sadri Ghazal 
Published: August 2020

Abstract
Objectives
The prevalence of endometriosis among women of childbearing age is about 10–15%. Although many patients with endometriosis do not have any symptoms, they may suffer from dysmenorrhea, dyspareunia, and chronic pelvic pain. our aim of this study is to investigate the relationship between the severity of symptoms of endometriosis with the spread of disease on ultrasonography as well as the stage of the disease.
Results
Regarding the various analyzes were performed, the cumulative size of posterior DIE less than 1 cm significantly correlated with minimal severity of dyspareunia and chronic pelvic pain. The incidence of dyspareunia was more in patients with complete stenosis of Douglas pouch comparing with cases with incomplete Douglas pouch stenosis. The incidence of severe and very severe pain in patients with Douglas pouch stenosis is relatively higher than patients without stenosis. This study demonstrated only dyspareunia is related to the stage of endometriosis and patients with dyspareunia are 5 times more at risk of a higher stage of the disease.
Association between Afterhours Discharge from the Intensive Care Unit and Hospital Mortality: A Multi-Centre Retrospective Cohort Study
Authors: Hall Adam , Wang Xioaming , Zuege Danny J , Opgenorth Dawn , Scales Damon C , Stelfox Henry T , Bagshaw Sean M 
Published: August 2020

Abstract
Background: There is conflicting evidence on the association between afterhours discharge from the intensive care unit (ICU) and hospital mortality. We examined the effects of afterhours discharge, including the potential effect of residual organ dysfunction, on hospital mortality in a large integrated health region. Methods: We performed a multi-centre retrospective cohort study of 10,463 adults discharged from nine mixed medical/surgical ICUs in Alberta from June 2012 to December 2014. We applied a two-stage modelling strategy to investigate the association between afterhours discharge (19:00h to 07:59h) and post-ICU hospital mortality. We applied mixed-effect multi-variable linear regression to assess the relationship between discharge organ dysfunction and afterhours discharge. We then applied mixed-effect multi-variable logistic regression to evaluate the direct, indirect and integrated associations of afterhours discharge on hospital mortality and hospitalization duration. Results: Of 10,463 patients, 23.7% (n=2,480) were discharged afterhours, of which 27.4% occurred on a holiday or weekend. This varied significantly by ICU size, type, and site. Patients discharged afterhours were more likely medical admissions, had greater multi-morbidity and illness acuity. Greater SOFA score in the 72 hours prior to discharge was not associated with afterhours discharge; however, was associated with hospital mortality (adjusted-OR 1.23; 95%CI,1.18-1.28). Afterhours discharge was associated with higher hospital mortality (adjusted-OR 1.19; 95%CI, 1.01-1.39), increased hospital stay (adjusted-OR 1.10; 95%CI,1.09-1.11) and increased post-ICU stay (adjusted-OR 1.16; 95%CI,1.14-1.17).Conclusions: Afterhours discharge is common, occurring in 1 in 4 discharges, and is widely variable across ICUs. Patients discharged afterhours have greater risk of hospital mortality and prolonged hospitalization.
The Associations of Active Travel to School with Physical Activity and Screen Time Among Adolescents: Do Individual and Parental Characteristics Matter?
Authors: Huang Caihong , Memon Aamir Raoof , Liang Chaomei , Hong Jin-Tao , Lin Youliang , Chen Si-Tong 
Published: August 2020

Abstract
Background: Little is known about the relationship of active travel to school (ATS) with physical activity (PA) and screen time (ST) by individual and parental characteristics among adolescents, especially in China. To address the research gap, this study aimed to explore the difference of sex, age, geographical location, parental occupation and education level in the relationship of ATS with PA and ST among students of grades 7-12 using cross-sectional data.Methods: In 13 cities of Hubei province, China, students from 39 public schools were recruited to engage in the survey. In total, 5898 students (response rate = 89.6%) were invited into this study. Participants were required to report their ATS (including its types), PA and ST as well as sociodemographic information using validated questionnaire. Descriptive analyses were used to report the information of all variables. Regression models were used to analyze the relationships of ATS and its types with PA and ST.Results: In a total of 4128 participants (boy: 50.9%; younger adolescents: 61.9%) included in the final analysis, the proportion of those with ATS was 47.3%. Regarding the types of ATS, walking accounted for over 30%, while cycling was 13.2%. Participants with ATS were more likely to have sufficient PA (OR = 1.26, 95% CI: 1.14-1.39), especially among boys, younger adolescents and those with lower parental education level. However, ATS was not associated with ST (OR = 0.94, 95% CI: 0.86-1.01). Participants with cycling had higher odds ratio of being physically active (OR for cycling = 1.47, 95% CI: 1.27-1.70; OR for walking = 1.18, 95% CI: 1.06-1.32). The association of ATS types with PA and ST differed by gender, age, geographical location and parental educational level as well as occupations.Conclusions: ATS may be a useful approach to increase PA among adolescents, but this should be explained by individual and parental characteristics.
Comparison of deep learning with regression analysis in creating predictive models for SARS-CoV-2 outcomes
Authors: Abdulaal Ahmed , Patel Aatish , Charani Esmita , Denny Sarah , Alqahtani Saleh , Davies Gary W , Mughal Nabeela , Moore Luke SP 
Published: August 2020

Abstract
Background Accurately predicting patient outcomes in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could aid patient management and allocation of healthcare resources. There are a variety of methods which can be used to develop prognostic models, ranging from logistic regression and survival analysis to more complex machine learning algorithms and deep learning. Despite several models having been created for SARS-CoV-2, most of these have been found to be highly susceptible to bias. We aimed to develop and compare two separate predictive models for death during admission with SARS-CoV-2.MethodBetween March 1 - April 24, 2020, 398 patients were identified with laboratory confirmed SARS-CoV-2 in a London teaching hospital. Data from electronic health records were extracted and used to create two predictive models using: 1) a Cox regression model and 2) an artificial neural network (ANN). Model performance profiles were assessed by validation, discrimination, and calibration.Results Both the Cox regression and ANN models achieved high accuracy (83.8%, 95% confidence interval (CI): 73.8 - 91.1 and 90.0%, 95% CI: 81.2 - 95.6, respectively). The area under the receiver operator curve (AUROC) for the ANN (92.6%, 95% CI: 91.1 - 94.1) was significantly greater than that of the Cox regression model (86.9%, 95% CI: 85.7 - 88.2), p=0.0136. Both models achieved acceptable calibration with Brier scores of 0.13 and 0.11 for the Cox model and ANN, respectively. ConclusionWe demonstrate an ANN which is non-inferior to a Cox regression model but with potential for further development such that it can learn as new data becomes available. Deep learning techniques are particularly suited to complex datasets with non-linear solutions, which make them appropriate for use in conditions with a paucity of prior knowledge. Accurate prognostic models for SARS-CoV-2 can provide benefits at the patient, departmental and organisational level.