bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceptions of provider’s epistemic authority in response to variant of uncertain significance‐related recommendations
Authors: Makhnoon Sukh , Mork Maureen , Arun Banu , Volk Robert J. , Peterson Susan K. 
Published: October 2020

AbstractUncertain genetic information such as variants of uncertain significance (VUS) is often encountered by patients in clinical cancer genetic testing. Although healthcare providers facilitate patient's understanding of VUS‐associated empirical risk and its medical implications, patients’ understanding and perceptions of risk often differ and may be based on subjective evaluations such as their perception of provider's epistemic authority (EA). This study examines the hypothesis that individuals attribute greater EA to genetic counselors (GCs) (compared to gastrointestinal oncologists) and to providers who recommend more active VUS‐related recommendations (compared to inactive). In a factorial experiment, 652 adult participants recruited on Amazon Mechanical Turk were block‐randomized to read one of 10 different types of VUS‐related scenarios in the context of colon cancer (5 recommendation types × 2 provider types). GCs were attributed higher EA than gastrointestinal oncologists (p = <.001). Active recommendations (comprehensive, check back, wrong) were attributed lower EA (M = 3.67, SD = 0.79) compared to the inactive (stand by, disregard) (M = 3.89, SD = 0.67) (p‐value = <.001). The wrong recommendation was attributed lowest EA compared to the four correct recommendations (mean difference = −0.34, −0.45, −0.35, and −0.44, respectively; p = .002), which, when dropped from the analysis, showed no difference between the correct active and inactive recommendations (3.78 vs. 3.89, p = .095). The higher EA attributed to GCs is encouraging and possibly explained by increased public awareness of the genetic counseling profession. The lack of difference in EA attributed to various correct, yet incomplete forms of VUS‐related recommendation indicates that individuals may be unaware of and thus completely rely on providers for complex medical topics like VUS. Communicating VUS‐related uncertainty warrants caution and further research to elucidate best practices and outcomes.
Predictive testing for neurodegenerative diseases in the age of next‐generation sequencing
Authors: Goldman Jill , Xie Shanghong , Green Dina , Naini Ali , Mansukhani Mahesh M. , Marder Karen 
Published: October 2020

AbstractThe availability and cost of next‐generation sequencing (NSG) now allow testing large numbers of genes simultaneously. However, the gold standard for predictive testing has been to test only for a known family mutation or confirmed family disease. The goal of this study was to investigate the psychological impact of predictive testing for autosomal dominant neurodegenerative diseases without a known family mutation using next‐generation sequencing panels compared to single‐gene testing of a known family mutation. Fourteen individuals from families with a known mutation and 10 individuals with unknown family mutations participated. Participants completed questionnaires on demographics, genetic knowledge, and psychological measures of anxiety, depression, perceived personal control, rumination, and intolerance to uncertainty at baseline and 1 and 6 months after receiving results. Decision regret was measured 1 and 6 months after receiving results. Participants completed a modified Huntington disease genetic testing protocol with genetic counseling and neurological and psychological evaluation. Genetic testing of either the known family mutation or an NGS panel of neurodegenerative disease genes was performed. Semi‐structured interviews were performed at 6 months post‐results about their experience. Two‐sample t tests were performed on data collected at each time point to identify significant between‐group differences in demographic variables, baseline psychological scores, and baseline genetic knowledge scores. Within‐group change over time was assessed by a mixed‐effects model. Results of this study indicate that NGS panels for predictive testing for neurodegenerative disease are safe and beneficial to participants when performed within a modified HD protocol. Though significant differences in psychological outcomes were found, these differences may have been driven by genetic results and baseline psychological differences between individuals within the groups. Participants did not regret their decision to test and were largely pleased with the testing protocol.
An instantaneous symmetrical component active power theory with finite control state based model predictive control strategy for distribution STATCOM
Authors: Sahu Gokulananda , Patjoshi Rajesh Kumar , Panigrahi Rakhee 
Published: October 2020

AbstractIn this paper, a novel Instantaneous Symmetrical Component Active Power theory with Finite Control State based Model Predictive Control (ISCAP‐FCS‐MPC) strategy is proposed for Distribution‐Static‐Compensator (DSTATCOM) to enhance the power quality in distribution system. Unlike conventional control schemes, the proposed method does not necessitate modulators for switching pulse creation and the discrete implementation of the system is offered to assess the forthcoming actions of the source currents for each of the eight achievable switching actions. These switching actions are generated with regard to turbulences raised in load as well as source sides. The proposed methodology is justified via MATLAB/Simulink environment and a relative analysis is realized through conventional Proportional Integral based Hysteresis Current Control (PI‐HCC) strategy. Further, an experimental investigation is accomplished with consideration of Real‐Time Digital Simulator (RTDS) incorporated with front end processor Xilinx spartan‐3 XC3S5000.
Sex‐specific peripheral and central responses to stress‐induced depression and treatment in a mouse model
Authors: Deonaraine Kristina K. , Wang Qian , Cheng Haoxiang , Chan Kenny L. , Lin Hsiao‐Yun , Liu Kalena , Parise Lyonna F. , Cathomas Flurin , Leclair Katherine B. , Flanigan Meghan E. , Li Long , Aleyasin Hossein , Guevara Christopher , Hao Ke , Zhang Bin , Russo Scott J. , Wang Jun 
Published: October 2020

AbstractMajor depressive disorder affects ~20% of the world population and is characterized by strong sexual dimorphism with females being two to three times more likely to develop this disorder. Previously, we demonstrated that a combination therapy with dihydrocaffeic acid and malvidin‐glucoside to synergistically target peripheral inflammation and stress‐induced synaptic maladaptation in the brain was effective in alleviating chronic social defeat stress (CSDS)‐induced depression‐like phenotype in male mice. Here, we test the combination therapy in a female CSDS model for depression and compared sex‐specific responses to stress in the periphery and the central nervous system. Similar to male mice, the combination treatment is also effective in promoting resilience against the CSDS‐induced depression‐like behavior in female mice. However, there are sex‐specific differences in peripheral immune responses and differential gene regulation in the prefrontal cortex to chronic stress and to the treatment. These data indicate that while therapeutic approaches to combat stress‐related disorders may be effective in both sexes, the mechanisms underlying these effects differ, emphasizing the need for inclusion of both sexes in preclinical studies using animal models.
Laser‐Induced Differentiation of Human Adipose‐Derived Stem Cells to Temporomandibular Joint Disc Cells
Authors: Karic Vesna , Chandran Rahul , Abrahamse Heidi 
Published: October 2020

Background and ObjectivesTemporomandibular disorder (TMD) is an incapacitating disease with temporomandibular joint (TMJ) disc degenerative changes in patients. Despite several research attempts to find a definitive treatment, there is no evidence of a permanent solution. The objective of the current study was to observe the role of 660 nm diode laser in the differentiation of human adipose‐derived stem cells (ADSCs) to fibroblasts and chondrocytes.Study Design/Materials and MethodsAfter irradiation, the morphology, viability, and adenosine triphosphate (ATP) proliferation of the ADSCs were analyzed at different time intervals. The differentiation of ADSCs toward fibroblastic and chondrogenic phenotypes was supported using flow cytometry and immunofluorescence at 1‐ and 2‐week post‐irradiation.ResultsMore than 90% of viable cells were observed in all experimental groups, with an increase in ATP proliferation. Flow cytometry analyses and immunofluorescence demonstrated the presence of chondrogenic and fibroblastic cell surface markers at 1‐ and 2‐week post‐irradiation.ConclusionThis study has demonstrated methods to induce the differentiation of ADSCs toward fibroblastic and chondrogenic phenotypes with a 660 nm diode laser. The study also proposes a future alternative method of treatment for patients with degenerative TMJ disc disorders and presents a positive prospect in the application of photobiomodulation and ADSCs in the treatment of degenerative TMJ disc. Lasers Surg. Med. © 2020 Wiley Periodicals LLC
Accuracy of weighted CTDI in estimating average dose delivered to CTDI phantoms: An experimental study
Authors: Treb Kevin , Li Ke 
Published: October 2020

PurposeThe concept of the weighted computed tomography dose index () was proposed in 1995 to represent the average CTDI across an axial section of a cylindrical phantom. The purpose of this work was to experimentally re‐examine the validity of the underlying assumptions behind for modern MDCT systems.MethodsTo enable experimental mapping of in the axial plane, in‐house 16 and 32 cm cylindrical phantoms were fabricated to allow the pencil chamber to reach any arbitrary axial location within the phantoms. The phantoms were scanned on a clinical MDCT with five beam collimation widths, three bowtie filters, and four kV levels. To evaluate the linearity and rotational invariance assumptions implicitly made when the weighting factors of 1/3 and 2/3 in the formula were originally derived, was measured at different radial and angular locations within the phantom for different collimation, bowtie, and kV combinations. The average CTDI () across the axial plane was calculated from the experimental two‐dimensional (2D) dose distribution and was compared with the traditional .ResultsFor both phantoms under all scan conditions, the axial dose distributions were found to have significant angular dependence, potentially due to the x‐ray attenuation by the patient couch or the head holder. The radial dose profiles were also found to significantly deviate from linearity in many cases due to the presence of the bowtie filter. When only the 12 o’clock peripheral and the traditional weighting factors were used to calculate , the average dose was overestimated in the 16 cm phantom by up to 8.4% at isocenter and up to 35.3% when the phantom was off‐centered by 6 cm; in the 32 cm phantom at isocenter, the average dose was overestimated by up to 12.8%. Using an average of the four peripheral measurements at the 12, 3, 6, and 9 o’clock locations reduced the error of to within 1.2% in the 16 cm phantom. For the 32 cm phantom, even by using the average of the peripheral measurements, the traditional underestimated the average dose by up to 4.3% due to aggressive drop‐off of the at the phantom periphery.ConclusionsThe linearity and rotational‐invariance assumptions behind the traditional formalism may not be valid for modern CT systems and thus may not accurately represent the average dose or radiation output within a CTDI phantom. Utilizing data from all four peripheral locations always improves accuracy of in representing the true average dose. For the large (32 cm) phantom, nonlinear models and more measurement points are needed if a more precise estimation of the average axial dose is required.
Results of the IX International Spectroradiometer Intercomparison and impact on precise measurements of new photovoltaic technologies
Authors: Pavanello D. , Galleano R. , Zaaiman W. , Ankit M. , Kouremeti N. , Gröbner J. , Hoogendijk K. , Po M. , Lisbona E.F. , Alius W. , Dosenovicova D. , Kroeger I. , Friedrich D. , Haverkamp E. , Minuto A. , Celi E. , Pravettoni M. , Bellenda G. , Fucci R. 
Published: October 2020

AbstractToday's variety of photovoltaic (PV) technologies imposes new challenges to laboratories and industries to precisely measure the performance of devices and, consequently, to accurately estimate the energy yield once installed in a specific location. Spectroradiometry has become a key discipline for metrology applied to PV: Spectral irradiance is one of the three parameters according to which solar simulators are classified according to IEC 60904‐9; precise spectrum measurements are a key factor in the spectral mismatch calculation. Finally, energy rating calculations according to IEC 61853 involve spectral irradiance conditions different than the AM1.5G standard spectrum. To tackle these issues, since 2011, the International Spectroradiometer Interlaboratory Comparison (ISRC) takes place annually in different locations of Europe with the participation of laboratories, research institutes, and industry partners to assess spectral measurement capabilities and share good measurement practices and protocols. In this paper, several results of the 9th ISRC 2019 are presented, looking in particular at the impact on characterization of new technologies like organic devices (OPV), dye‐sensitized (DSSC), and perovskites.
Intravenous pulses of methylprednisolone for infants with severe bronchopulmonary dysplasia and respiratory support after 3 months of age
Authors: Billion Elodie , Hadchouel Alice , Garcelon Nicolas , Delacourt Christophe , Drummond David 
Published: October 2020

AbstractIntroductionThere are few published data on the efficacy of systemic corticosteroids in preterm infants with very severe forms of bronchopulmonary dysplasia (BPD), requiring respiratory support after 3 months of age. The aim of this study was to report the use of pulses of methylprednisolone in this population and its consequences on the level of respiratory support.MethodsThis retrospective monocentre study included infants over 3 months of age with severe BPD who received at least one pulse of methylprednisolone (300 mg/m2/day intravenous [IV] over 3 days). The primary outcome was the evolution of the pulmonary severity score (PSS) during the 3 months preceding and the 5 months following the first pulse. The evolution of the median PSS over time was analyzed using linear segmented regression for interrupted time series.ResultsTen infants were included. During the 3 months preceding the first pulse, a significant increase in the median PSS was observed (p = .01), followed by a progressive decrease during the 5 months after administration of the first pulse (p < .01). Greater effects were observed in more severe infants requiring mechanical or noninvasive ventilation than in those receiving supplemental oxygen through nasal cannula.ConclusionHigh‐dose IV pulses of methylprednisolone were associated with a decrease in the level of respiratory support required by infants with very severe forms of BPD, with a greater effect in those on mechanical or noninvasive ventilation. Further studies are warranted to confirm these preliminary results and assess the long‐term safety of this therapy.
Transcriptome profiling and functional analysis suggest that the constitutive overexpression of four cytochrome P450s confers resistance to abamectin in Tetranychus urticae from China
Authors: Xu Dandan , Zhang Yan , Zhang Youjun , Wu Qingjun , Guo Zhaojiang , Xie Wen , Zhou Xiaomao , Wang Shaoli 
Published: October 2020

AbstractBACKGROUNDThe two‐spotted spider mite Tetranychus urticae is a polyphagous and cosmopolitan pest that has developed high resistance to abamectin, making it difficult to control. Although ‘target resistance’ related to glutamate‐gated chloride channel mutations was found in T. urticae field populations in China, other resistance mechanisms appear to be involved. Here, we conducted genome‐wide transcriptome profiling using RNA‐sequencing of two abamectin‐resistant populations (NB‐ZJ and SY‐BJ) and one susceptible strain (Lab‐SS) to identify differentially expressed genes that might contribute to the resistance of T. urticae to abamectin in China.RESULTSOur experiments showed that abamectin resistance was synergized by piperonyl butoxide (PBO) and triphenyl phosphate (TPP), with synergistic ratios (SR) of 2.95‐fold and 2.21‐fold for PBO and 3.55‐fold and 2.84‐fold for TPP in NB‐ZJ and SY‐BJ populations, respectively. Transcriptome data and quantitative real‐time PCR (qRT‐PCR) revealed that seven detoxification enzyme genes were overexpressed in the two resistant populations. Furthermore, functional analysis by RNA interference (RNAi) indicated that the mortality caused by abamectin was significantly increased by the separate silencing of the P450 genes CYP389C10, CYP392D8, CYP392A11, and CYP392A12.CONCLUSIONqRT‐PCR expression and RNAi data suggest that the overexpression of P450 genes CYP389C10, CYP392D8, CYP392A11, and CYP392A12 may be involved in the abamectin‐resistance of field populations of T. urticae in China. This knowledge could facilitate the elucidation of resistance mechanisms and the development of resistance management of T. urticae field populations. © 2020 Society of Chemical Industry
The focal hepatic hot spot sign with lung cancer in computed tomography
Authors: Hoang Van Trung , Vo Nhu Quynh , Trinh Cong Thao , Nguyen Hoang Quan , Chansomphou Vichit , Le Trong Binh 
Published: October 2020

Key messageThe focal hepatic hot spot sign appears as an area of intense focal wedge‐shaped enhancement of the quadrate lobe (segment IVa) of the liver in the arterial and venous phase. If this sign appears on enhanced computed tomography of the abdomen, obstruction of thoracic central venous must be considered, especially when clinical symptoms are unclear.
Chromosomal copy number analysis of products of conception by conventional karyotyping and next‐generation sequencing
Authors: Tamura Yuki , Santo Mitsuo , Araki Yasuhisa , Matsubayashi Hidehiko , Takaya Yukiko , Kitaya Kotaro , Doshida Masakazu , Yamaguchi Kohei , Mizuta Shimpei , Takahashi Chie , Kim Namhyo , Okuno Koichiro , Takeuchi Takumi , Ishikawa Tomomoto 
Published: October 2020

AbstractPurposeChromosomal abnormalities are a major cause of spontaneous abortion, and conventional G‐banded karyotyping (G‐banding) is mainly utilized for chromosomal analysis. Recently, next‐generation sequencing (NGS) has been introduced for chromosomal analysis. Here, we aimed to investigate the applicability and utility of NGS‐based chromosomal analysis of products of conception (POC) on chorionic villus samples from spontaneous abortion.MethodsThe results of chromosomal analysis of 7 chorionic villus samples from spontaneous abortion were compared between conventional G‐banding and NGS‐based chromosomal copy number analysis. Age dependency and frequency of each chromosomal aneuploidy were evaluated for 279 cases analyzed by NGS.ResultsExcluding two cases (culture failure and maternal cell contamination), the results were consistent between G‐banding and NGS. For cases analyzed by NGS, the rate of chromosomal abnormality increased in a maternal age‐dependent manner. The frequency of each chromosomal aneuploidy detected by NGS was almost the same as that previously reported. Finally, NGS analysis was possible for difficult cases by G‐banding analysis, such as culture failure, maternal cell contamination, long‐term storage cases, and low cell number.ConclusionsChromosome analysis using NGS not only obtains comparable results to conventional G‐banding, but also can analyze POC more accurately and efficiently.
Hydroxy Piperlongumines: Synthesis, Antioxidant, Cytotoxic Effect on Human Cancer Cell Lines, Inhibitory Action and ADMET Studies
Authors: Subramani Muthuraman , Ramamoorthy Gayathri , Hemaiswarya Shanmugam , Waidha Kamran , Brindha J. , Balamurali M. M. , Doble Mukesh , Rajendran Saravanakumar 
Published: October 2020

AbstractPiperlongumine, an anticancer natural product, was sequentially demethylated to obtain mono, di and trihydroxy piperlongumines. The hydroxy piperlongumines were evaluated for cytotoxic effect on five human cancer cell lines (kidney, skin, cervical, breast, and lung) in vitro. Results from cytotoxic and cell growth inhibition studies showed monohydroxy piperlongumine (MHPL) was the most active of the series, exhibited potent cytotoxic effect on all the tested cell lines. Flow cytometry analysis shows MHPL inhibited cell cycle progression of HeLa cells in the S phase, indicated that it inhibits the DNA replication process. Fluorescence staining indicated MHPL treated cells were apoptotic. MHPL upregulated the apoptotic genes (caspase‐3, −8, Bax, and p53) and downregulated anti‐apoptotic gene (Bcl‐2). The antioxidant study revealed that hydroxy piperlongumines are effective antioxidants. For MHPL, all the molecular descriptors were within the range of 95 % of approved drugs suggesting high druglikeliness. Our results suggest that MHPL could be developed as a potential lead molecule for cancer therapy.
Synthesis, Characterization, Antimicrobial Evaluation, and Computational Investigation of Substituted Imidazo[2,1‐b][1,3,4]Thiadiazole Derivatives
Authors: Dagli Meltem , Er Mustafa , Karakurt Tuncay , Onaran Abdurrahman , Alici Hakan , Tahtaci Hakan 
Published: October 2020

AbstractIn this study, a novel series of 2,6‐disubstituted and 2,5,6‐trisubstituted imidazo[2,1‐b][1,3,4]thiadiazole derivatives were synthesized starting from 2‐amino‐1,3,4‐thiadiazole derivatives. Structures of the synthesized compounds were characterized using various analysis techniques. Then, in vitro biological activity tests were carried out for all synthesized compounds and they were found to show moderate to good activity against all bacteria and fungi tested. Next, molecular docking simulations were performed to observe the inhibition effect of the synthesized compounds on the 3R9C receptor and support their biological activity results. Finally, the pharmacokinetic, ADME and toxicity properties of all compounds were examined using FAF‐Drugs and ProTox webservers and it was concluded that they had acceptable toxicity and ADME properties.