bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid Determination of Listeria monocytogenes by Automated Enzyme-Linked Immunoassay and Nonradioactive DNA Probe
Authors: Kerdahi Khalil F , Istafanos Philip F 
Published: March 2020

Abstract
A rapid and reliable analytical method was developed to detect and confirm the presence of Listeria monocytogenes in raw and partially processed foods. Forty-nine food samples (25 mixed cut vegetable salad, 12 smoked salmon, and 12 sterile smoked salmon) were individually inoculated with high levels [10–100 colony forming units (cfu)/25 g sample] and low levels (1–10 cfu/25 g sample) of L. monocytogenes, and were screened using the Vitek Immuno Diagnostic Assay (VIDAS) Listeria monocytogenes (VIDAS LMO)]. Positive test results were confirmed as L. monocytogenes by nonradioactive DNA probe. All samples inoculated with high levels of L. monocytogenes were detected by VIDAS and 96% were confirmed as L. monocytogenes by DNA probe. VIDAS LMO detected 89% of samples inoculated with low levels of L. monocytogenes, and 87% of these were confirmed as positive by DNA probe. In addition, 12 other samples (4 from each of mixed cut vegetable salad, smoked salmon, and sterile smoked salmon) were inoculated with high levels of L. ivanovii, L. seeligeri, L. welshimeri, L. innocua, L. grayi, and L. murrayi. Samples were assayed by the same protocol and all gave negative results. Compared with the cultural method, the VIDAS LMO nonradioactive DNA probe combination is highly specific, discriminates between L. monocytogenes and all other Listeria species, and reduces analytical time.
Determination of Fumonisins B1 and B2 in Various Maize Products by a Combined SAX + C18 Column and Immunoaffinity Column
Authors: Möller Tord E , Gustavsson Håkan F 
Published: March 2020

Abstract
Fumonisins B1 and B2 were determined in 42 samples of different maize products from the Swedish market by 2 different methods based on cleanup steps using an immunoaffinity column and a combination of SAX + C18 columns, respectively. A simple “precipitation step” was included before the samples were added to the main column(s), giving less column clogging, fewer interfering peaks, and better recoveries for the different sample matrixes. Recovery, repeatability, and results from the survey showed comparable results with the methods. The limit of detection for both methods was 5 μg/kg for fumonisin B1 and 10 μg/kg for fumonisin B2. All 7 maize chips analyzed and 6 of 8 popcorn samples contained fumonisins (B1 + B2) with averages of 180 and 115 μg/kg, respectively. All other samples except a maize flour sample contained little or no fumonisins.
Development and Validation of a Method for Direct Analysis of Aflatoxins in Animal Feeds by Ultra-High-Performance Liquid Chromatography with Fluorescence Detection
Authors: Kumar Anup , Dhanshetty Manisha , Banerjee Kaushik 
Published: March 2020

Abstract

Background and Objective
Aflatoxin (AF) contamination is one of the major regulatory concerns for animal feed. As feed is a complex analytical matrix, validated methods on AFs in feed are scanty. The available methods involve a derivatization step before AF analysis by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). The aim of this study was thus to develop and validate a simple and rapid method for direct analysis of AFs (AFB1, AFB2, AFG1, AFG2) in a range of animal feed matrices.

Methods
Feed samples were extracted with 80% methanol, followed by dilution with water and immmunoaffinity column cleanup. AFs were estimated using an ultra-high performance liquid chromatography (UHPLC) instrument. Use of a large volume flow cell in FLD allowed direct analysis of all AFs with high sensitivity. The method was thoroughly validated in a range of feed matrices.

Results
This sample preparation workflow minimized co-extractives, along with matrix interferences. In pigeon pea husk feed, the method provided a limit of quantification (LOQ) of 0.5 ng/g for each AF with recoveries of AF- B1, B2, G1, and G2 as 71.5, 75.6, 82.4, and 78.2%, respectively. The precision (relative standard deviation, RSD) was below 5%. A similar method performance was also recorded in other matrices, including wheat bran feed and poultry feed.

Conclusions
The optimized method is suitable for regulatory testing because it is simple, robust, cost-effective, and high throughput in nature, with high sensitivity and selectivity.

Highlights
Our workflow has provided a straightforward method for the analysis of AFs in a wide range of animal feed matrices with high sensitivity, selectivity, throughput, and cost-effectiveness. The method allowed a direct analysis of AFs by UHPLC-FLD without a step of derivatization.
Analyzing Molecular Basis of Heat-Induced Loss-of-Wheat Resistance to Hessian Fly (Diptera: Cecidomyiidae) Infestation Using RNA-Sequencing
Authors: Zhu Lieceng , Yuan Jiazheng (John) , O’Neal Jordan , Brown Daria , Chen Ming-Shun 
Published: March 2020

AbstractHeat stress compromises wheat resistance to Hessian fly (HF, Mayetiola destructor (Say)) (Diptera: Cecidomyiidae) infestation. The objective of this research is to analyze the molecular basis of heat-induced loss of wheat resistance to HF infestation using RNA Sequencing (RNA-seq). To this end, two resistant wheat cultivars ‘Molly’ and ‘Caldwell’ containing the resistance genes H13 and H6, respectively, were infested with an avirulent HF biotype GP and treated with different temperatures to examine the impact of heat stress on their resistance phenotypes. Tissue samples collected from HF feeding sites in Molly plants were subjected to RNA-seq analysis to determine the effect of heat stress on transcript expression of genes in wheat plants. Our results indicate that resistance to HF infestation in Caldwell is more sensitive to heat stress than that in Molly, and that heat stress down-regulates most genes involved in primary metabolism and biosynthesis of lignin and cuticular wax, but up-regulate most or all genes involved in auxin and 12-oxo-phytodienoic acid (OPDA) signaling pathways. Our results and previous reports suggest that heat stress may impair the processes in wheat plants that produce and mobilize chemical resources needed for synthesizing defensive compounds, weaken cell wall and cuticle defense, decrease OPDA signaling, but increase auxin signaling, leading to the suppressed resistance and activation of susceptibility.
Severe Insect Pest Impacts on New Zealand Pasture: The Plight of an Ecological Outlier
Authors: Goldson Stephen L , Barker Gary M , Chapman Hazel M , Popay Alison J , Stewart Alan V , Caradus John R , Barratt Barbara I P 
Published: March 2020

AbstractNew Zealand’s intensive pastures, comprised almost entirely introduced Lolium L. and Trifolium L. species, are arguably the most productive grazing-lands in the world. However, these areas are vulnerable to destructive invasive pest species. Of these, three of the most damaging pests are weevils (Coleoptera: Curculionidae) that have relatively recently been controlled by three different introduced parasitoids, all belonging to the genus Microctonus Wesmael (Hymenoptera: Braconidae). Arguably that these introduced parasitoids have been highly effective is probably because they, like many of the exotic pest species, have benefited from enemy release. Parasitism has been so intense that, very unusually, one of the weevils has now evolved resistance to its parthenogenetic parasitoid. This review argues that New Zealand’s high exotic pasture pest burden is attributable to a lack of pasture plant and natural enemy diversity that presents little biotic resistance to invasive species. There is a native natural enemy fauna in New Zealand that has evolved over millions of years of geographical isolation. However, these species remain in their indigenous ecosystems and, therefore, play a minimal role in creating biotic resistance in the country’s exotic ecosystems. For clear ecological reasons relating to the nature of New Zealand pastures, importation biological control can work extremely well. Conversely, conservation biological control is less likely to be effective than elsewhere.
Otodectes cynotis (Sarcoptiformes: Psoroptidae): New Records on Wild Carnivores in Brazil With a Case Report
Authors: Huang-Bastos Matheus , Bassini-Silva Ricardo , Rolim Luna Scarpari , OConnor Barry , Ochoa Ronald , Barros-Battesti Darci Moraes , Jacinavicius Fernando de Castro 
Published: March 2020

AbstractThe genus Otodectes Canestrini 1894, comprises an unique species, Otodectes cynotis (Hering, 1838), that is responsible for the ‘ear mange’ in terrestrial carnivores worldwide. Here we report its occurrence in four Brazilian host species from the southeastern region, based on records from Carnivora in the Acari Collection of the Butantan Institute, São Paulo state (IBSP). We also register a case report of a crab-eating fox, Cerdocyon thous L. 1758 found parasitized by this mite species.
The positive impact of lockdown in Wuhan on containing the COVID-19 outbreak in China
Authors: Lau Hien , Khosrawipour Veria , Kocbach Piotr , Mikolajczyk Agata , Schubert Justyna , Bania Jacek , Khosrawipour Tanja 
Published: March 2020

Abstract

Background
With its epicenter in Wuhan, China, the COVID-19 outbreak was declared a Public Health Emergency of International Concern by the World Health Organization (WHO). Consequently, many countries have implemented flight restrictions to China. China itself has imposed a lockdown of the population of Wuhan as well as the entire Hubei province. However, whether these two enormous measures have led to significant changes in the spread of COVID-19 cases remains unclear.

Methods
We analyzed the available data on the development of confirmed domestic and international COVID-19 cases before and after lockdown measures. We evaluated the correlation of domestic air traffic to the number of confirmed COVID-19 cases and determined the growth curves of COVID-19 cases within China before and after lockdown as well as after changes in COVID-19 diagnostic criteria.

Results
Our findings indicate a significant increase in doubling time from 2 days (95% CI: 1.9–2.6) to 4 days (95% CI: 3.5–4.3), after imposing lockdown. A further increase is detected after changing diagnostic and testing methodology to 19.3 (95% CI: 15.1–26.3), respectively. Moreover, the correlation between domestic air traffic and COVID-19 spread became weaker following lockdown (before lockdown: r = 0.98, P &lt; 0.05 vs after lockdown: r = 0.91, P = NS).

Conclusions
A significantly decreased growth rate and increased doubling time of cases was observed, which is most likely due to Chinese lockdown measures. A more stringent confinement of people in high risk areas seems to have a potential to slow down the spread of COVID-19.
Pennycress, carbon wise: labeling experiments reveal how pennycress seeds efficiently incorporate carbon into biomass
Authors: Sedbrook John C , Durrett Timothy P 
Published: March 2020

This article comments on:
Tsogtbaatar E, Cocuron J-C, Alonso AP. 2020. Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high biosynthetic efficiency. Journal of Experimental Botany 71, 3037–3051.
Radio view of a broad-line Type Ic supernova ASASSN-16fp
Authors: A. J. Nayana , Chandra Poonam 
Published: March 2020

ABSTRACT
We present extensive radio observations of a Type Ic supernova, ASASSN-16fp. Our data represent the lowest frequency observations of the supernova beyond 1000 d with a frequency range of 0.33–25 GHz and a temporal range of ∼8–1136 d post-explosion. The observations are best represented by a model of synchrotron emission from a shocked circumstellar shell initially suppressed by synchrotron self-absorption. Assuming equipartition of energy between relativistic particles and magnetic fields, we estimate the velocity and radius of the blast wave to be $v$ ∼ 0.15c and r ∼ 3.4 × 1015 cm, respectively, at t0 ∼ 8 d post-explosion. We infer the total internal energy of the radio-emitting material evolves as E ∼ 0.37 × 1047 (t/t0)0.65 erg. We determine the mass-loss rate of the progenitor star to be $\dot{M} \sim (0.4\!-\!3.2) \times 10^{-5}\, \mathrm{M}_{\odot }\, \rm yr^{-1}$ at various epochs post-explosion, consistent with the mass-loss rate of Galactic Wolf–Rayet stars. The radio light curves and spectra show a signature of density enhancement in the circumstellar medium at a radius of ∼1.10 × 1016 cm from the explosion centre.
Turbulence in stratified atmospheres: implications for the intracluster medium
Authors: Mohapatra Rajsekhar , Federrath Christoph , Sharma Prateek 
Published: March 2020

ABSTRACT
The gas motions in the intracluster medium (ICM) are governed by turbulence. However, since the ICM has a radial profile with the centre being denser than the outskirts, ICM turbulence is stratified. Stratified turbulence is fundamentally different from Kolmogorov (isotropic, homogeneous) turbulence; kinetic energy not only cascades from large to small scales, but it is also converted into buoyancy potential energy. To understand the density and velocity fluctuations in the ICM, we conduct high-resolution (10242 × 1536 grid points) hydrodynamical simulations of subsonic turbulence (with rms Mach number $\mathcal {M}\approx 0.25$) and different levels of stratification, quantified by the Richardson number Ri, from Ri = 0 (no stratification) to Ri = 13 (strong stratification). We quantify the density, pressure, and velocity fields for varying stratification because observational studies often use surface brightness fluctuations to infer the turbulent gas velocities of the ICM. We find that the standard deviation of the logarithmic density fluctuations (σs), where s = ln (ρ/ &lt; ρ($z$) &gt;), increases with Ri. For weakly stratified subsonic turbulence (Ri ≲ 10, $\mathcal {M}\lt 1$), we derive a new σs–$\mathcal {M}$–Ri relation, $\sigma _\mathrm{ s}^2=\ln (1+b^2\mathcal {M}^4+0.09\mathcal {M}^2 \mathrm{Ri} H_\mathrm{ P}/H_\mathrm{ S})$, where b = 1/3–1 is the turbulence driving parameter, and HP and HS are the pressure and entropy scale heights, respectively. We further find that the power spectrum of density fluctuations, P(ρk/ &lt; ρ &gt;), increases in magnitude with increasing Ri. Its slope in k-space flattens with increasing Ri before steepening again for Ri ≳ 1. In contrast to the density spectrum, the velocity power spectrum is invariant to changes in the stratification. Thus, we find that the ratio between density and velocity power spectra strongly depends on Ri, with the total power in density and velocity fluctuations described by our σs–$\mathcal {M}$–Ri relation. Pressure fluctuations, on the other hand, are independent of stratification and only depend on $\mathcal {M}$.
Semi-analytic forecasts for JWST – III. Intrinsic production efficiency of Lyman-continuum radiation
Authors: Yung L Y Aaron , Somerville Rachel S , Popping Gergö , Finkelstein Steven L 
Published: March 2020

ABSTRACT
The James Webb Space Telescope is expected to enable transformational progress in studying galaxy populations in the very early Universe, during the epoch of reionization. A critical parameter for understanding the sources that reionized the Universe is the Lyman-continuum production efficiency, ξion, defined as the rate of production of ionizing photons divided by the intrinsic UV luminosity. In this work, we combine self-consistent star formation and chemical enrichment histories predicted by semi-analytic models of galaxy formation with stellar population synthesis (SPS) models to predict the expected dependence of ξion on galaxy properties and cosmic epoch from z = 4–10. We then explore the sensitivity of the production rate of ionizing photons, $\dot{N}_\text{ion}$, to the choice of SPS model and the treatment of stellar feedback in our galaxy formation model. We compare our results to those of other simulations, constraints from empirical models, and observations. We find that adopting SPS models that include binary stars predict about a factor of 2 more ionizing radiation than models that only assume single stellar populations. We find that UV-faint, low-mass galaxies have values of ξion about 0.25 dex higher than those of more massive galaxies, but find weak evolution with cosmic time, about 0.2 dex from z ∼ 12–4 at fixed rest-UV luminosity. We provide predictions of $\dot{N}_\text{ion}$ as a function of Mh and a number of other galaxy properties. All results presented in this work are available at https://www.simonsfoundation.org/semi-analytic-forecasts-for-jwst/.
Testing tidal alignment models for anisotropic correlations of halo ellipticities with N-body simulations
Authors: Okumura Teppei , Taruya Atsushi , Nishimichi Takahiro 
Published: March 2020

ABSTRACT
There is a growing interest of using the intrinsic alignment (IA) of galaxy images as a tool to extract cosmological information complimentary to galaxy clustering analysis. Recently, Okumura & Taruya derived useful formulas for the intrinsic ellipticity–ellipticity correlation, the gravitational shear–intrinsic ellipticity correlation, and the velocity–intrinsic ellipticity correlation functions based on the linear alignment (LA) model. In this paper, using large-volume N-body simulations, we measure these alignment statistics for dark-matter haloes in real and redshift space and compare them to the LA and non-linear alignment model predictions. We find that anisotropic features of baryon acoustic oscillations in the IA statistics can be accurately predicted by our models. The anisotropy due to redshift-space distortions (RSDs) is also well described in the large-scale limit. Our results indicate that one can extract the cosmological information encoded in the IA through the Alcock–Paczynski and RSD effects.
Realistic gravitational focusing of meteoroid streams
Authors: Moorhead Althea V , Clements Tiffany D , Vida Denis 
Published: March 2020

ABSTRACT
The number density and flux of a meteoroid stream is enhanced near a massive body due to the phenomenon known as gravitational focusing. The greatest enhancement occurs directly opposite the massive body from the stream radiant: as an observer approaches this location, the degree of focusing is unbound for a perfectly collimated stream. However, real meteoroid streams exhibit some dispersion in radiant and speed that will act to eliminate this singularity. In this paper, we derive an analytic approximation for this smoothing that can be used in meteoroid environment models and is based on real measurements of meteor shower radiant dispersion.
Relaxation of NO+ by collision with para-H2 (j = 0)
Authors: Cabrera-González L D , Páez-Hernández D , Denis-Alpizar O 
Published: March 2020

ABSTRACT
The first tentative detection of the nitrosylium ion (NO+) in the interstellar medium (ISM) was reported just a few years ago. The application of non-local thermal equilibrium models requires the knowledge of the collisional rate coefficients with the most common colliders in the ISM (e.g. He, H, H2, and e). The main goals of this paper are to study the collision of the NO+ molecule with para-H2 (j = 0) and report the rate coefficients for the lower rotational states of NO+. A large set of ab initio energies was computed at the CCSD(T)/aug-cc-pV5Z level of theory. A new potential energy surface averaged over the H2 orientations was then fitted using a reproducing kernel Hilbert space procedure. The state-to-state cross-sections of NO++para-H2 (j = 0) for the first 18 rotational levels were computed using the close-coupling method. The rotational rate coefficients of this system were compared with those for NO++He, and a different propensity rule was found. Furthermore, the hyperfine rate coefficients were also calculated using the infinite-order-sudden scaling procedure.
Atmospheric compositions and observability of nitrogen-dominated ultra-short-period super-Earths
Authors: Zilinskas Mantas , Miguel Yamila , Mollière Paul , Tsai Shang-Min 
Published: March 2020

ABSTRACT
We explore the chemistry and observability of nitrogen-dominated atmospheres for ultra-short-period super-Earths. We base the assumption that super-Earths could have nitrogen-filled atmospheres on observations of 55 Cancri e that favour a scenario with a high-mean-molecular-weight atmosphere. We take Titan’s elemental budget as our starting point and using chemical kinetics compute a large range of possible compositions for a hot super-Earth. We use analytical temperature profiles and explore a parameter space spanning orders of magnitude in C/O and N/O ratios, while always keeping nitrogen the dominant component. We generate synthetic transmission and emission spectra and assess their potential observability with the future James Webb Space Telescope (JWST) and ARIEL. Our results suggest that HCN is a strong indicator of a high C/O ratio, which is similar to what is found for H-dominated atmospheres. We find that these worlds are likely to possess C/O &gt; 1.0, and that HCN, CN, and CO should be the primary molecules to be searched for in thermal emission. For lower temperatures (T &lt; 1500 K), we additionally find NH3 in high N/O ratio cases, and C2H4 and CH4 in low N/O ratio cases to be strong absorbers. Depletion of hydrogen in such atmospheres would make CN, CO, and NO exceptionally prominent molecules to look for in the 0.6–5.0 $\rm{\mu m}$ range. Our models show that the upcoming JWST and ARIEL missions will be able to distinguish atmospheric compositions of ultra-short-period super-Earths with unprecedented confidence.
Rampant Misexpression in a Mimulus (Monkeyflower) Introgression Line Caused by Hybrid Sterility, Not Regulatory Divergence
Authors: Kerwin Rachel E , Sweigart Andrea L 
Published: March 2020

AbstractDivergence in gene expression regulation is common between closely related species and may give rise to incompatibilities in their hybrid progeny. In this study, we investigated the relationship between regulatory evolution within species and reproductive isolation between species. We focused on a well-studied case of hybrid sterility between two closely related yellow monkeyflower species, Mimulus guttatus and Mimulus nasutus, that is caused by two epistatic loci, hybrid male sterility 1 (hms1) and hybrid male sterility 2 (hms2). We compared genome-wide transcript abundance across male and female reproductive tissues (i.e., stamens and carpels) from four genotypes: M. guttatus, M. nasutus, and sterile and fertile progeny from an advanced M. nasutus–M. guttatus introgression line carrying the hms1–hms2 incompatibility. We observed substantial variation in transcript abundance between M. guttatus and M. nasutus, including distinct but overlapping patterns of tissue-biased expression, providing evidence for regulatory divergence between these species. We also found rampant genome-wide misexpression, but only in the affected tissues (i.e., stamens) of sterile introgression hybrids carrying incompatible alleles at hms1 and hms2. Examining patterns of allele-specific expression in sterile and fertile introgression hybrids, we found evidence for interspecific divergence in cis- and trans-regulation, including compensatory cis–trans mutations likely to be driven by stabilizing selection. Nevertheless, species divergence in gene regulatory networks cannot explain the vast majority of the gene misexpression we observe in Mimulus introgression hybrids, which instead likely manifests as a downstream consequence of sterility itself.