bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing feedforward and recurrent neural network architectures with human behavior in artificial grammar learning
Authors: Alamia Andrea , Gauducheau Victor , Paisios Dimitri , VanRullen Rufin 
Published: December 2020

AbstractIn recent years artificial neural networks achieved performance close to or better than humans in several domains: tasks that were previously human prerogatives, such as language processing, have witnessed remarkable improvements in state of the art models. One advantage of this technological boost is to facilitate comparison between different neural networks and human performance, in order to deepen our understanding of human cognition. Here, we investigate which neural network architecture (feedforward vs. recurrent) matches human behavior in artificial grammar learning, a crucial aspect of language acquisition. Prior experimental studies proved that artificial grammars can be learnt by human subjects after little exposure and often without explicit knowledge of the underlying rules. We tested four grammars with different complexity levels both in humans and in feedforward and recurrent networks. Our results show that both architectures can “learn” (via error back-propagation) the grammars after the same number of training sequences as humans do, but recurrent networks perform closer to humans than feedforward ones, irrespective of the grammar complexity level. Moreover, similar to visual processing, in which feedforward and recurrent architectures have been related to unconscious and conscious processes, the difference in performance between architectures over ten regular grammars shows that simpler and more explicit grammars are better learnt by recurrent architectures, supporting the hypothesis that explicit learning is best modeled by recurrent networks, whereas feedforward networks supposedly capture the dynamics involved in implicit learning.
CO2 utilization as a soft oxidant for the synthesis of styrene from ethylbenzene over Co3O4 supported on magnesium aluminate spinel: role of spinel activation temperature
Authors: Madduluri Venkata Rao , Marella Ravi Kumar , Hanafiah Marlia M. , Lakkaboyana Sivarama Krishna , Suresh babu G. 
Published: December 2020

AbstractMagnesium aluminate spinel (MgAl2O4) supported Co3O4 catalysts are synthesized and tested for the oxidative dehydrogenation (ODH) of ethylbenzene using CO2 as a soft oxidant. The effect of spinel calcination temperature on the catalytic performance has been systematically investigated. With an increase in the activation temperature from 600 to 900 °C, the active presence of a single-phase MgAl2O4 spinel is observed. A catalyst series consisting of MgAl2O4 spinel with varying Co loadings (10–20 wt%) were prepared and systematically distinguished by ICP, XRD, BET, TPR, NH3-TPD, UV–Vis DRS, FT-IR, XPS, SEM, and TEM. Among the tested cobalt catalysts, 15Co/800MA sample derived by calcination of MgAl2O4 support at 800 °C exhibits the most excellent catalytic performance with the maximum ethylbenzene conversion (≥ 82%). Also, high yields of styrene (≥ 81%) could be consistently achieved on the same active catalyst. Further, the catalyst exhibited almost stable activity during 20 h time-on-stream with a slow decrease in the ethylbenzene conversion from 82 to 59%. However, the selectivity of styrene (98%) stayed almost constant during the reaction. Activation of the MgAl2O4 spinel at 800 °C facilitates a dramatic chemical homogeneity for the alignment of Co3O4 nanoparticles on the surface of the active catalyst. Moreover, the isolated Co3O4 clusters have a strong chemical/electronic interaction with the Mg2+ and Al3+ ions on the support perform a crucial role to achieve the maximum catalytic activity.
The neuroprotective effects of activated α7 nicotinic acetylcholine receptor against mutant copper–zinc superoxide dismutase 1-mediated toxicity
Authors: Ito Taisei , Inden Masatoshi , Ueda Tomoyuki , Asaka Yuta , Kurita Hisaka , Hozumi Isao 
Published: December 2020

AbstractAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective and progressive loss of motor neurons. Although many drugs have entered clinical trials, few have shown effectiveness in the treatment of ALS. Other studies have shown that the stimulation of α7 nicotinic acetylcholine receptor (nAChR) can have neuroprotective effects in some models of neurodegenerative disease, as well as prevent glutamate-induced motor neuronal death. However, the effect of α7 nAChR agonists on ALS-associated mutant copper–zinc superoxide dismutase 1 (SOD1) aggregates in motor neurons remains unclear. In the present study, we examined whether α7 nAChR activation had a neuroprotective effect against SOD1G85R-induced toxicity in a cellular model for ALS. We found that α7 nAChR activation by PNU282987, a selective agonist of α7 nAChR, exhibited significant neuroprotective effects against SOD1G85R-induced toxicity via the reduction of intracellular protein aggregates. This reduction also correlated with the activation of autophagy through the AMP-activated protein kinase (AMPK)–mammalian target of rapamycin (mTOR) signaling pathway. Furthermore, the activation of α7 nAChRs was found to increase the biogenesis of lysosomes by inducing translocation of the transcription factor EB (TFEB) into the nucleus. These results support the therapeutic potential of α7 nAChR activation in diseases that are characterized by SOD1G85R aggregates, such as ALS.
The effect of changes in cardiovascular activity on corneal biomechanics and pulsation in rabbits
Authors: Antończyk Agnieszka , Kubiak-Nowak Dominika , Borawski Wojciech , Kiełbowicz Zdzisław , Danielewska Monika E. 
Published: December 2020

AbstractThe aim was to assess the relationships between cardiovascular activity, corneal pulse characteristics, and corneal biomechanics in rabbits. Seventeen rabbits were randomly assigned to one of two anesthetic regimens to induce differences in arterial blood pressure and heart rate. Experimental protocol included measuring blood flow parameters in the ophthalmic artery by color Doppler imaging, corneal biomechanical parameters using a non-contact tonometer Corvis ST, and the corneal pulse (CP) signal using a non-contact ultrasonic technique. Statistically significantly lower mean values of normalized amplitudes of higher CP harmonics and changes in eight of the twelve corneal biomechanical parameters were observed in the rabbit group with lower arterial blood pressure and higher heart rate, intraocular pressure, and resistive index. The results of partial correlations showed that the CP signal energy and amplitude of its first harmonic correlate with the resistive index, diastolic and mean arterial pressures, whereas no statistically significant correlation was found between any of the CP parameters and intraocular pressure. Our pilot study indicates, for the first time, that non-contact and continuous measuring of corneal pulse allows indirectly assessing changes in cardiovascular activity when the confounding effect of intraocular pressure is eliminated.
Syllable-first rather than letter-first to improve phonemic awareness
Authors: Vazeux Maria , Doignon-Camus Nadège , Bosse Marie-Line , Mahé Gwendoline , Guo Teng , Zagar Daniel 
Published: December 2020

AbstractThe present study investigates the nature of the spelling-to-sound correspondences taught to enhance phonemic awareness in prereaders. The main assumption in the literature is that learning the alphabetic code through letter-to-phoneme correspondences is the best way to improve phonemic awareness. The alternative syllabic bridge hypothesis, based on the saliency and early availability of syllables, assumes that learning to associate letters to phonological syllables enables phoneme units to be the mirror of the letters and to become accessible, thereby developing phonemic awareness of prereaders. A total of 222 French-speaking prereaders took part in a 4-session learning program based on correspondences either between letters and syllables (letters-to-syllable group) or between letters and phonemes (letter-to-phoneme group), and the fifth last session on coding and decoding. Our results showed a greater increase in phonemic awareness in the letters-to-syllable group than in the letter-to-phoneme group. The present study suggests that teaching prereaders letters-to-syllable correspondences is a key to successful reading.
Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption
Authors: Svenning Jon Brage , Dalheim Lars , Vasskog Terje , Matricon Lucie , Vang Birthe , Olsen Ragnar Ludvig 
Published: December 2020

AbstractCell wall disruption is necessary to maximize lipid extraction yields in conventional species of mass-cultivated microalgae. This study investigated the effect of sonication, solvent choice and number of extractions on the lipid yield, lipid class composition and fatty acid composition of the diatom Porosira glacialis. For comparison, the diatom Odontella aurita and green alga Chlorella vulgaris were included in the study. Sonication effectively disrupted P. glacialis cells, but did not increase the total lipid yield compared to physical stirring (mixing). In all three microalgae, the content of membrane-associated glyco- and phosopholipids in the extracted lipids was strongly dependent on the solvent polarity. A second extraction resulted in higher yields from the microalgae only when polar solvents were used. In conclusion, choice of solvent and number of extractions were the main factors that determined lipid yield and lipid class composition in P. glacialis.
Multiple random phosphorylations in clock proteins provide long delays and switches
Authors: Upadhyay Abhishek , Marzoll Daniela , Diernfellner Axel , Brunner Michael , Herzel Hanspeter 
Published: December 2020

AbstractTheory predicts that self-sustained oscillations require robust delays and nonlinearities (ultrasensitivity). Delayed negative feedback loops with switch-like inhibition of transcription constitute the core of eukaryotic circadian clocks. The kinetics of core clock proteins such as PER2 in mammals and FRQ in Neurospora crassa is governed by multiple phosphorylations. We investigate how multiple, slow and random phosphorylations control delay and molecular switches. We model phosphorylations of intrinsically disordered clock proteins (IDPs) using conceptual models of sequential and distributive phosphorylations. Our models help to understand the underlying mechanisms leading to delays and ultrasensitivity. The model shows temporal and steady state switches for the free kinase and the phosphoprotein. We show that random phosphorylations and sequestration mechanisms allow high Hill coefficients required for self-sustained oscillations.
The long non coding RNA H19 as a biomarker for breast cancer diagnosis in Lebanese women
Authors: Elias-Rizk Tamina , El Hajj Joelle , Segal-Bendirdjian Evelyne , Hilal George 
Published: December 2020

AbstractBreast cancer is the most common cancer in women worldwide. Minimally invasive percutaneous image-guided biopsies are the current cornerstone in the diagnosis of breast lesions detected on mammography/ultrasonography/MRI or palpable clinically. However, apparently benign breast disease seen on benign biopsies is a limiting factor for diagnosis and a risk factor of breast cancer especially in the high-risk category patients. Hypothesizing that molecular changes often occur before morphological variations, the levels of the LncRNA H19 were measured in anonymous tissues obtained from 79 women’s image guided breast biopsies, and correlated with cancer progression and aggressiveness. Using a double-blinded approach, H19 might be attributed an interesting role of a more sensitive biomarker in core breast biopsies, independently of the radiological/clinical classification and distant from the clinical management. We established different thresholds for H19 levels in normal versus proliferative, versus malignant tissues. Additionnally, H19 could act as an intra-group risk marker categorizing the biopsies in normal versus benign, versus precancerous breast tissue, and as a prognostic factor in cancerous lesions discriminating aggressive versus nonaggressive lesions. Our study suggests that the lncRNA H19 could be a potential marker for breast cancer diagnosis, prognosis and risk management.
Effect of IL-34 on T helper 17 cell proliferation and IL-17 secretion by peripheral blood mononuclear cells from rheumatoid arthritis patients
Authors: Li Xin , Lei Yimeng , Gao Ziyu , Zhang Bei , Xia Liping , Lu Jing , Shen Hui 
Published: December 2020

AbstractInterleukin (IL)-34 is a new pro-inflammatory cytokine with elevated expression in rheumatoid arthritis (RA) patients. Our previous study showed that the frequency of T helper 17 (Th17) cells was also elevated in RA patients. Our study aimed to determine the effects of IL-34 on the proliferation, transcription factor expression and cytokine secretion of different subgroups of CD4 + T cells [Th1, Th2, Th17 and regulatory T (Treg) cells] in RA patients. Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of 10 RA patients and stimulated with different concentrations of recombinant human (rh) IL-34 (0, 25, 50 and 100 ng/ml). Flow cytometry was used to determine the frequencies of the 4 subgroups of CD4 + T cells. Reverse transcription-PCR, western blotting and enzyme-linked immunosorbent assays were used to determine the mRNA and protein expression levels of transcription factors and cytokines. As a result, the frequency of Th17 cells was obviously increased under IL-34 stimulation. Moreover, the expression of the transcription factor retinoic acid-related orphan receptor (ROR-γt) and secretion of IL-17 by PBMCs were increased by stimulation with IL-34. However, there were no effects of IL-34 on transcription factors or cytokine secretion in Th1, Th2 and Treg cells. In conclusion, IL-34 can improve the proliferation of Th17 cells and expression of IL-17 in RA patients.
Molecular patterns of isolated tubulitis differ from tubulitis with interstitial inflammation in early indication biopsies of kidney allografts
Authors: Hruba Petra , Madill-Thomsen Katelynn , Mackova Martina , Klema Jiri , Maluskova Jana , Voska Ludek , Parikova Alena , Slatinska Janka , Halloran Philip F. , Viklicky Ondrej 
Published: December 2020

AbstractThe Banff 2019 kidney allograft pathology update excluded isolated tubulitis without interstitial inflammation (ISO-T) from the category of borderline (suspicious) for acute T cell-mediated rejection due to its proposed benign clinical outcome. In this study, we explored the molecular assessment of ISO-T. ISO-T or interstitial inflammation with tubulitis (I + T) was diagnosed in indication biopsies within the first 14 postoperative days. The molecular phenotype of ISO-T was compared to I + T either by using RNA sequencing (n = 16) or by Molecular Microscope Diagnostic System (MMDx, n = 51). RNA sequencing showed lower expression of genes related to interferon-y (p = 1.5 *10–16), cytokine signaling (p = 2.1 *10–20) and inflammatory response (p = 1.0*10–13) in the ISO-T group than in I + T group. Transcripts with increased expression in the I + T group overlapped significantly with previously described pathogenesis-based transcript sets associated with cytotoxic and effector T cell transcripts, and with T cell-mediated rejection (TCMR). MMDx classified 25/32 (78%) ISO-T biopsies and 12/19 (63%) I + T biopsies as no-rejection. ISO-T had significantly lower MMDx scores for interstitial inflammation (p = 0.014), tubulitis (p = 0.035) and TCMR (p = 0.016) compared to I + T. Fewer molecular signals of inflammation in isolated tubulitis suggest that this is also a benign phenotype on a molecular level.
CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome
Authors: Simora Rhoda Mae C. , Xing De , Bangs Max R. , Wang Wenwen , Ma Xiaoli , Su Baofeng , Khan Mohd G. Q. , Qin Zhenkui , Lu Cuiyu , Alston Veronica , Hettiarachchi Darshika , Johnson Andrew , Li Shangjia , Coogan Michael , Gurbatow Jeremy , Terhune Jeffery S. , Wang Xu , Dunham Rex A. 
Published: December 2020

AbstractCRISPR/Cas9-based gene knockout in animal cells, particularly in teleosts, has proven to be very efficient with regards to mutation rates, but the precise insertion of exogenous DNA or gene knock-in via the homology-directed repair (HDR) pathway has seldom been achieved outside of the model organisms. Here, we succeeded in integrating with high efficiency an exogenous alligator cathelicidin gene into a targeted non-coding region of channel catfish (Ictalurus punctatus) chromosome 1 using two different donor templates (synthesized linear dsDNA and cloned plasmid DNA constructs). We also tested two different promoters for driving the gene, zebrafish ubiquitin promoter and common carp β-actin promoter, harboring a 250-bp homologous region flanking both sides of the genomic target locus. Integration rates were found higher in dead fry than in live fingerlings, indicating either off-target effects or pleiotropic effects. Furthermore, low levels of mosaicism were detected in the tissues of P1individuals harboring the transgene, and high transgene expression was observed in the blood of some P1fish. This can be an indication of the localization of cathelicidin in neutrophils and macrophage granules as also observed in most antimicrobial peptides. This study marks the first use of CRISPR/Cas9 HDR for gene integration in channel catfish and may contribute to the generation of a more efficient system for precise gene integration in catfish and other aquaculture species, and the development of gene-edited, disease-resistant fish.
DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
Authors: Al-Ogaili Adil S. , Liyanage Rohana , Lay Jack O. , Jiang Tieshan , Vuong Christine N. , Agrawal Shilpi , Kumar Thallapuranam Krishnaswamy Suresh , Berghman Luc R. , Hargis Billy M. , Kwon Young Min 
Published: December 2020

AbstractSeveral agonists to CD40 have shown to induce acquired immune responses. Here, we developed and evaluated the rolling circle amplification (RCA) products that are based on anti-CD40 DNA aptamers as a novel vaccine adjuvant. First, we developed DNA aptamers with specific binding affinity to chicken CD40 extra domain (chCD40ED). Next, we prepared the RCA products that consist of these aptamers to increase the spanning space and overall binding affinity to chCD40ED. Using 8 DNA aptamer candidates, 4 aptamer-based RCA products (aptamer RCAs) were generated, each consisting of two distinct aptamers. We demonstrated that all 4 aptamer RCAs significantly induced the signal transduction in chicken HD11 macrophage cell line (p < 0.05). Finally, we conjugated one of the aptamer RCAs (Aptamer RCA II) to M2e epitope peptide of influenza virus as a model hapten, and the immune complex was injected to chickens. Aptamer RCA II stimulated anti-M2e IgG antibody production to the level significantly higher as compared to the control (M2e epitope alone; p < 0.05). The results of our work suggest that aptamer RCA is a novel platform to boost the efficacy of vaccines, which might find broad applications to other antigens beyond M2e epitope evaluated in this study using chicken infection model.
Importance and Qualitative Requirements of Magnetic Resonance Imaging for Therapy Planning in Rectal Cancer – Interdisciplinary Recommendations of AIO, ARO, ACO and the German Radiological Society
Authors: Attenberger Ulrike Irmgard , Clasen Stephan , Ghadimi Michael , Grosse Ulrich , Antoch Gerald , Schreyer Andreas G. , Wessling Johannes , Hausmann Daniel , Piso Pompiliu , Plodeck Verena , Stintzing Sebastian , Rödel Claus Michael , Hofheinz Ralf Dieter 
Published: December 2020

Due to its excellent intrinsic soft tissue contrast, magnetic resonance imaging allows excellent visualization and anatomical separation of therapy-relevant risk structures such as the mesorectal fascia, local lymph nodes, and vascular structures in patients with rectal carcinoma. This makes magnetic resonance imaging (MRI) a valuable evaluation method for further therapeutic stratification. In particular, MRI is indispensable for the decision to refrain from neoadjuvant therapy and to choose a primary surgical approach. In addition to the oncologically generally relevant T-, N-, and M-criteria, two further parameters are included: the extramural vascular infiltration and the circumferential resection margin. Due to the significant impact of MRI on further therapeutic decision-making, standardized MR image quality is considered essential.
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The impact of a short-term stay abroad on L2 Spanish syntactic complexity development in narratives
Authors: Long Avizia Y. , Solon Megan 
Published: December 2020

AbstractGiven the notable increase in participation in short-term (e.g., eight weeks or less) study abroad, especially in the US, recent empirical work on the role of context in second language (L2) learning has sought to investigate the impact of a short-term stay abroad on language development. The present study examined English-speaking learners’ syntactic complexity development in oral narratives after a four-week stay abroad. With regard to three measures of syntactic complexity (length of analysis of speech [AS]-units, number of clauses per AS-unit, length of clause), findings revealed that the study abroad group demonstrated no statistically significant change over the study period. However, individual-level analyses revealed that over half of the study abroad learners increased complexity in narratives in terms of clause length. Further, half of the study abroad learners exhibited increases in syntactic complexity on at least two of the three syntactic complexity measures examined.
Kinetic analysis of the wrist and fingers during fastball and curveball pitches
Authors: Shibata Shohei , Kageyama Masahiro , Inaba Yuki , Yoshioka Shinsuke , Fukashiro Senshi 
Published: December 2020

AbstractThis study had two objectives: (a) revealing kinetic parameter differences at the fingers during a fastball and curveball, and (b) examining timing control between the wrist and finger torques. The participants were eight baseball pitchers. The kinetics of the wrist and fingers were calculated using an inverse dynamics method. The peak torque and work of finger adduction during the curveball was significantly larger than that during the fastball. During the fastball pitch, the maximal correlation coefficient between the wrist flexion torque and finger flexion torque was very high (r = 0.94 ± 0.05). The reasons for this result are twofold: (a) the extrinsic finger muscles cross the wrist (biarticular muscle) and (b) the wrist flexion torque during the fastball pitch acts in the direction of acceleration of the ball. During the curveball pitch, we found two typical types of wrist and finger torque control. Furthermore, the two pitchers exerted large wrist extension and radial torque, and finger adduction torque. Although the other six pitchers hardly exerted these torques, they exerted wrist flexion torque predominantly. It was considered that the six pitchers selected wrist flexion torque as the control for the fastball and curveball pitch to confuse the batter.