bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of short-term training on pastoral community employment creation and livelihood improvement: a study on selected Ethiopian pastoral areas
Authors: Shiferaw Robson Mekonnin 
Published: September 2020

AbstractThis study investigated the effects of a short-term training on pastoral community employment creation and livelihood improvement. The study investigated the perception of beneficiaries pertinent to the benefits of a short-term training scholarship, experiences of graduate trainees, estimation of employability rate after training, and types of employment TVET graduates are engaged during the year 2014 to 2017 in Pastoral Resilience Improvement through Market Extension project operating clusters. Self-administered survey questionnaires were used and distributed to 128 project beneficiaries in Afar, Eastern, and Southern clusters of Ethiopia. Since the instrument was close-ended questionnaires in the form of a dichotomous, and quantitative research approach was deployed. The quantitative part of the assessment was analyzed using descriptive and inferential statistics with the support of SPSS version 20. The finding of the study indicated that the reasons for unemployment of the beneficiaries were mainly lack of financial support and poor industry linkage with employers in the areas. On the other hand, such activities should be assessed once again for financial and material support since the majority of the trainees were unemployed and seeking support besides the training received from the program in all clusters from all concerned development partners. The finding of the study depicted, gender-related issues in all clusters implies that women involvements or participation shows a variation in some clusters. However, the project more benefited male beneficiaries than women in all clusters. Therefore, if the project will continue this type of program, balancing gender and equal benefit should be maintained in all clusters. Redesigning the program is required because due to providing training alone cannot bring livelihood changes as the finding of the study revealed. This is because the beneficiaries of the program were selected from an economically poor family background. Therefore, the program should evaluate once again for financial and equipment support since the majority of the trainees were unemployed and seeking support from different development stakeholders in the three clusters.
Soil microbiota manipulation and its role in suppressing soil-borne plant pathogens in organic farming systems under the light of microbiome-assisted strategies
Authors: De Corato Ugo 
Published: September 2020

AbstractSoil microbiota plays a key role in suppressing soil-borne plant pathogens improving the natural soil suppressiveness. Microbiome disturbance triggers specific perturbation to change and shape the soil microbial communities’ network for increasing suppression against phytopathogens and related diseases. Very important goals have been reached in manipulation of soil microbiota through agronomical practices based on soil pre-fumigation, organic amendment, crop rotation and intercropping. Nevertheless, to limit inconsistencies, drawbacks and failures related to soil microbiota disturbance, a detailed understanding of the microbiome shifts during its manipulation is needed under the light of the microbiome-assisted strategies. Next-generation sequencing often offers a better overview of the soil microbial communities during microbiomes manipulation, but sometime it does not provide information related to the highest taxonomic resolution of the soil microbial communities. This review work reports and discusses the most reliable findings in relation to a comprehensive understanding of soil microbiota and how its manipulation can improve suppression against soil-borne diseases in organic farming systems. Role and functionality of the soil microbiota in suppressing soil-borne pathogens affecting crops have been basically described in the first section of the paper. Characterization of the soil microbiomes network by high-throughput sequencing has been introduced in the second section. Some relevant findings by which soil microbiota manipulation can address the design of novel sustainable cropping systems to sustain crops’ health without use (or reduced use) of synthetic fungicides and fumigants have been extensively presented and discussed in the third and fourth sections, respectively, under the light of the new microbiome-assisted strategies. Critical comparisons on the next-generation sequencing have been provided in the fifth section. Concluding remarks have been drawn in the last section.
Thermodynamic and detailed kinetic study of the formation of orthophthalaldehyde-agmatine complex by fluorescence intensities
Authors: Kital Khémesse , Traoré Moumouny , Sarr Diégane , Mbaye Moussa , Seye Mame Diabou Gaye , Coly Atanasse , Delattre François , Tine Alphonse 
Published: September 2020

AbstractThe aim of this work is to determine the thermodynamic parameters and the kinetics of complex formation between orthophthalaldehyde (OPA) and agmatine (AGM) in an alkaline medium (pH 13). Firstly, the association constant (Ka) between orthophthalaldehyde and agmatine was determined at different temperatures (between 298 K and 338 K) with a step size of 10 K. Secondly, the thermodynamic parameters such as standard enthalpy (ΔH°), standard entropy (ΔS°),and Gibbs energy (∆G) were calculated, where a positive value ofΔH° (+45.50 kJ/mol) was found, which shows that the reaction is endothermic. In addition, the low value of ΔS°(+0.24 kJ/mol) indicates a slight increase in the disorder in the reaction medium. Furthermore, the negative values ofΔGbetween −35.62 kJ/mol and −26.02 kJ/mol show that the complex formation process is spontaneous. Finally, the parameters of the kinetics of the reaction between OPA and AGM were determined as follows: when the initial concentration ofAGM(5 × 10−6 M) is equal to that of the OPA, the results show that the reaction follows an overall 1.5 order kinetics with an initial rate of 5.1 × 10−7Mmin−1and a half-life of 8.12 min. The partial order found in relation to the AGM is 0.8. This work shows that the excess of OPA accelerates the formation reaction of the complex.
Genome-wide association analysis of idiopathic epilepsy in the Belgian shepherd
Authors: Belanger J. M. , Famula T. R. , Gershony L. C. , Palij M. K. , Oberbauer A. M. 
Published: September 2020

Abstract
Background
Idiopathic epilepsy (IE) is a common neurological disorder in the domestic dog, and is defined as repeated seizure activity having no identifiable underlying cause. Some breeds, such as the Belgian shepherd dog, have a greater prevalence of the disorder. Previous studies in this and other breeds have identified ADAM23 as a gene that confers risk of IE, although additional loci are known to exist. The present study sought to identify additional loci that influence IE in the Belgian shepherd dog.

Results
Genome-wide association studies (GWAS) revealed a significant association between IE and CFA 14 (p < 1.03 E− 08) and a suggestive association on CFA 37 (p < 2.91 E− 06) in a region in linkage disequilibrium with ADAM23. Logistic regression identified a 2-loci model that demonstrated interaction between the two chromosomal regions that when combined predicted IE risk with high sensitivity.

Conclusions
Two interacting loci, one each on CFAs 14 and 37, predictive of IE in the Belgian shepherd were identified. The loci are adjacent to potential candidate genes associated with neurological function. Further exploration of the region is warranted to identify causal variants underlying the association. Additionally, although the two loci were very good at predicting IE, they failed to capture all the risk, indicating additional loci or incomplete penetrance are also likely contributing to IE expression in the Belgian shepherd dog.
Reasons for and Consequences of Low Energy Availability in Female and Male Athletes: Social Environment, Adaptations, and Prevention
Authors: Wasserfurth Paulina , Palmowski Jana , Hahn Andreas , Krüger Karsten 
Published: September 2020

AbstractLow energy availability (LEA) represents a state in which the body does not have enough energy left to support all physiological functions needed to maintain optimal health. When compared to the normal population, athletes are particularly at risk to experience LEA and the reasons for this are manifold. LEA may result from altered dietary behaviours that are caused by body dissatisfaction, the belief that a lower body weight will result in greater performance, or social pressure to look a certain way. Pressure can also be experienced from the coach, teammates, and in this day and age through social media platforms. While LEA has been extensively described in females and female athletes have started fighting against the pressure to be thin using their social media platforms, evidence shows that male athletes are at risk as well. Besides those obvious reasons for LEA, athletes engaging in sports with high energy expenditure (e.g. rowing or cycling) can unintentionally experience LEA; particularly, when the athletes’ caloric intake is not matched with exercise intensity. Whether unintentional or not, LEA may have detrimental consequences on health and performance, because both short-term and long-term LEA induces a variety of maladaptations such as endocrine alterations, suppression of the reproductive axis, mental disorders, thyroid suppression, and altered metabolic responses. Therefore, the aim of this review is to increase the understanding of LEA, including the role of an athlete’s social environment and the performance effects related to LEA.
Partnerships with local organisations are vital for conducting health research in Syria—personal reflections
Authors: Ekzayez Abdulkarim 
Published: September 2020

AbstractThroughout the 9 years of the Syrian conflict, humanitarian actors have accumulated enormous experience of dealing with this complex environment and collected substantial data—only a tiny fraction, however, is being used in informing practice and research. Partnering with academic institutions could enable these actors to foster their efforts in data collection, analysis and research production. These partnerships should go beyond the traditional understanding of partnerships to include elements of a two-way capacity strengthening paradigm, and equal benefits and opportunities. Drawing on my personal experience of providing clinical medicine in field hospitals in conflict-affected areas in Syria, as well as my experience in the humanitarian response there working with Save the Children and a number of local organisations, and then moving to policy and research working with London School of Hygiene and Tropical Medicine, Chatham House and King’s College London, I conclude equal partnerships and effective collaborations between academic institutions and humanitarian actors could provide a concrete and sustainable approach of conducting health research in conflict settings.
Comparative transcriptomic analysis of the tea plant (Camellia sinensis) reveals key genes involved in pistil deletion
Authors: Liu Yufei , Pang Dandan , Tian Yiping , Li Youyong , Jiang Huibing , Sun Yunnan , Xia Lifei , Chen Linbo 
Published: September 2020

Abstract
Background
The growth process of the tea plant (Camellia sinensis) includes vegetative growth and reproductive growth. The reproductive growth period is relatively long (approximately 1.5 years), during which a large number of nutrients are consumed, resulting in reduced tea yield and quality, accelerated aging, and shortened economic life of the tea plant. The formation of unisexual and sterile flowers can weaken the reproductive growth process of the tea plant. To further clarify the molecular mechanisms of pistil deletion in the tea plant, we investigated the transcriptome profiles in the pistil-deficient tea plant (CRQS), wild tea plant (WT), and cultivated tea plant (CT) by using RNA-Seq.

Results
A total of 3683 differentially expressed genes were observed between CRQS and WT flower buds, with 2064 upregulated and 1619 downregulated in the CRQS flower buds. These genes were mainly involved in the regulation of molecular function and biological processes. Ethylene synthesis–related ACC synthase genes were significantly upregulated and ACC oxidase genes were significantly downregulated. Further analysis revealed that one of the WIP transcription factors involved in ethylene synthesis was significantly upregulated. Moreover, AP1 and STK, genes related to flower development, were significantly upregulated and downregulated, respectively.

Conclusions
The transcriptome analysis indicated that the formation of flower buds with pistil deletion is a complex biological process. Our study identified ethylene synthesis, transcription factor WIP, and A and D-class genes, which warrant further investigation to understand the cause of pistil deletion in flower bud formation.
Ishige okamurae reduces blood glucose levels in high-fat diet mice and improves glucose metabolism in the skeletal muscle and pancreas
Authors: Yang Hye-Won , Son Myeongjoo , Choi Junwon , Oh Seyeon , Jeon You-Jin , Byun Kyunghee , Ryu Bo Mi 
Published: September 2020

Abstract
Brown alga (Ishige okamurae; IO) dietary supplements have been reported to possess anti-diabetic properties. However, the effects of IO supplements have not been evaluated on glucose metabolism in the pancreas and skeletal muscle. C57BL/6 N male mice (age, 7 weeks) were arranged in five groups: a chow diet with 0.9% saline (NFD/saline group), high-fat diet (HFD) with 0.9% saline (HFD/saline group). high-fat diet with 25 mg/kg IO extract (HFD/25/IOE). high-fat diet with 50 mg/kg IO extract (HFD/50/IOE), and high-fat diet with 75 mg/kg IO extract (HFD/75/IOE). After 4 weeks, the plasma, pancreas, and skeletal muscle samples were collected for biochemical analyses. IOE significantly ameliorated glucose tolerance impairment and fasting and 2 h blood glucose level in HFD mice. IOE also stimulated the protein expressions of the glucose transporters (GLUTs) including GLUT2 and GLUT4 and those of their related transcription factors in the pancreases and skeletal muscles of HFD mice, enhanced glucose metabolism, and regulated blood glucose level. Our results suggest Ishige okamurae extract may reduce blood glucose levels by improving glucose metabolism in the pancreas and skeletal muscle in HFD-induced diabetes.
Elucidation of predictors of disease progression in patients with relapsing polychondritis at the onset: potential impact on patient monitoring
Authors: Shimizu Jun , Yamano Yoshihisa , Kawahata Kimito , Suzuki Noboru 
Published: September 2020

Abstract
Background
In patients with relapsing polychondritis (RP), organ involvement developed in those with progressive and/or long disease courses. For their management, elucidation of a subgroup suggesting disease progression is awaited.

Methods
We previously conducted a physician’s questionnaire-based retrospective study to elucidate major clinical features of Japanese patients with RP. We here evaluated organ involvement at disease onset and at the last follow-up. We then counted cumulative numbers of involved organs at the last follow-up in 229 RP patients and compared them with involved organ numbers at disease onset, as possible indicators of disease progression. We assigned their prognosis at the last follow-up into “patient prognostic stages” from no medication (stage 1) to death (stage 5). We utilized nonparametric tests for group comparisons.

Results
Involved organ numbers per-patient were 1.13 ± 0.03 at disease onset and 3.25 ± 0.10 at the last follow-up (disease duration was 4.69 ± 0.33 years), and increased along with the patient prognostic stages.
At disease onset, 135 and 48 patients had auricular involvement (59% of 229 patients, defined as auricular-onset subgroup; AO) and respiratory involvement (21%, respiratory-onset subgroup; RO), respectively. 46 patients presented with other conditions (20%, miscellaneous-onset subgroup; MO) including CNS, ocular, and inner ear involvement, among others.
RO patients showed worse (poorer) prognostic stages than AO patients. MO patients developed respiratory and/or auricular involvement thereafter and then showed significantly higher mortality rate (15%; 7/46) than AO patients (5.9%; 8/135).
In RP patients who did not develop respiratory involvement until the last follow-up (throughout the disease course; 117 patients), mortality rate was 19% in 26 MO patients and 3.3% in 91 AO patients. Accordingly, RO patients and MO patients associated with relatively poor prognosis compared with AO patients.

Conclusions
Allocation of patients to RO and MO subgroups was suggested to associate with poorer prognosis of RP than AO subgroups, especially AO subgroups without respiratory involvement throughout. All RP patients deserve careful monitoring but special attention should be paid to MO patients because of their diverse and accelerated disease progression.
Endovascular management of an unsual case of spontaneous Retroperitoneal Haemorrhage due to Fibromuscular Dysplasia
Authors: Molina-Nuevo Juan David , López-Martínez Lorena , Pedrosa-Jiménez María José , Juliá-Molla Enrique 
Published: September 2020

Abstract
Background
Fibromuscular dysplasia (FMD) is an uncommon vascular disease that results in stenosis, dissection or aneurysmal degeneration. However, it can sometimes manifest atypically, as we show in this case.

Case presentation
A 24-year old patient with no relevant medical history with severe left hypochondrium pain. The physical examination showed blood pressure levels of 160/90 mmHg. An abdominopelvic CT evidenced left retroperitoneal haematoma associated with active bleeding and left renal artery stenosis. Given these findings, it was decided to perform an endovascular treatment. Significant stenosis was seen during the arteriography in both renal arteries, suggesting fibromuscular dysplasia and development of a collateral neovascular network responsible for the retroperitoneal haematoma. It was embolised in association with angioplasty of the left renal artery. The patient had a favourable outcome; however, high blood pressure levels persisted. A new bilateral renal angioplasty was performed, which returned blood pressure values to normal. The patient was discharged without needing antihypertensives.

Conclusions
FMD is a rare disease that can show multiple clinical presentations and need individualized treatment options. Endovascular techniques are in the first therapeutic line regarding fibromuscular dysplasia.
Antiplasmodial activities and abortifacient properties of three commonly used African indigenous anti-malarial plants in Plasmodium berghei infected pregnant mice: implication for maternal and fetal health
Authors: Babalola Ayodele S. , Idowu Olufunmilayo A. , Ademolu Kehinde O. , Olukunle J. , Rahman Samson A. 
Published: September 2020

Abstract
Background
The use of herbs for malaria treatment is common among pregnant women in Nigeria. This study through a survey documented three most commonly used herbs in the management of pregnancy-associated malaria in Abeokuta, Nigeria. This study also evaluated the efficacy and abortifacient properties of the selected herbs against established Plasmodium berghei NK65 infection in 110 experimental pregnant mice randomly distributed into 22 groups and treated with extracts of Morinda lucida (L.) Benth. (Rubiaceae), Enantia chlorantha (oliv.) (Annonaceae), and Cymbopogon citatrus (Stapf) (Poaceae) at a graded dose of 100, 200, and 300 mg/kg and Sulphadoxine-pyrimethamine (Fansidar) at 10 mg/kg.

Results
From the survey, Cymbopogon citratus (Leaf), Morinda lucida (Leaf), and Enanthia chlorantha (Bark) were the most frequently mentioned to be useful in management of malaria during pregnancy. Both M. lucida and E. chlorantha produced up to 70% P. gerghei chemosuppression in a dose dependent manner. Chemosupression was less than 50% in mice treated with C. citratus extracts. E. chlorantha induced abortion, while extracts of C. citratus and M. lucida caused miscarriage in pregnant mice. Progesterone titters were reduced in mice treated with plant extracts compared with those administered with Fansidar and untreated groups (p < 0.05).

Conclusion
This study showed that M. lucida and E. chlorantha had anti-malarial properties, which are promising in reducing problems with pregnancy associated malaria in the face of growing resistance to currently available drugs. However, they are capable of inducing abortion by impairing the production of progesterone. In order to reduce the risk of danger posed by use of herbs in pregnancy on mothers and the developing fetus, there is need for proper awareness on the possible abortifacient and teratogenic properties of herbs used in management of malaria during pregnancy.
Variety of radiological findings in a family with COVID-19: a case report
Authors: Gharekhanloo Farideh , Sedighi Iraj , Khazaei Salman 
Published: September 2020

AbstractIntroductionIn December 2019, a case series of pneumonia associated with seafood and wet animal market was reported as coronavirus disease 2019 (COVID-19) in Wuhan, Hubei, China. The COVID-19 less frequently affects the pediatric group, and asymptomatic children are commonly reported. This feature is important due to the potential transmissibility of COVID-19 through these cases. There is a variety of atypical clinical symptoms and imaging manifestations among children infected with COVID-19.Case presentationWe report two sisters with COVID-19 pneumonia who had close contact with their 77-year-old grandmother. Their grandmother expired with a confirmed diagnosis of COVID-19. The older sister (15 years old) firstly presented with severe cough, fever, dyspnea, diarrhea, and lymphopenia. A chest computed tomography (CT) scan showed atypical lobar pneumonic infiltration only in the left lower lobe. The younger sister (6 years old) was affected the following day with milder symptoms (i.e., fever and cough). Her chest CT scan demonstrated typical mild generalized patchy ground-glass opacity with interlobular septal thickening scattered in both lungs. The CT presentation of their grandmother showed typical bilateral white lungs with crazy paving appearance. The standard regimen for both sisters was administrated, and the patients’ respiratory conditions improved after 5 days of treatment. Both cases were discharged with good general conditions.ConclusionChildren are considered potentially important causes of COVID-19 transmission. The symptoms in children are milder, and clinicians should be aware of taking a definite history of infected family members. Atypical clinical symptoms and imaging features are more common in children than adults; therefore, it is necessary to identify the typical and atypical imaging patterns of COVID-19 pneumonia in the pediatric population.