bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-nucleus RNA-seq reveals dysregulation of striatal cell identity due to Huntington’s disease mutations
Authors: Malaiya Sonia , Cortes-Gutierrez Marcia , Herb Brian R. , Coffey Sydney R. , Legg Samuel R.W. , Cantle Jeffrey P. , Colantuoni Carlo , Carroll Jeffrey B. , Ament Seth A. 
Published: July 2020

ABSTRACTHuntington’s disease (HD) is a dominantly inherited neurodegenerative disorder caused by a trinucleotide expansion in exon 1 of the huntingtin (Htt) gene. Cell death in HD occurs primarily in striatal medium spiny neurons (MSNs), but the involvement of specific MSN subtypes and of other striatal cell types remains poorly understood. To gain insight into cell type-specific disease processes, we studied the nuclear transcriptomes of 4,524 cells from the striatum of a genetically precise knock-in mouse model of the HD mutation,HttQ175/+, and from wildtype controls. We used 14-15-month-old mice, a time point roughly equivalent to an early stage of symptomatic human disease. Cell type distributions indicated selective loss of D2 MSNs and increased microglia in agedHttQ175/+mice. Thousands of differentially expressed genes were distributed across most striatal cell types, including transcriptional changes in glial populations that are not apparent from RNA-seq of bulk tissue. Reconstruction of cell typespecific transcriptional networks revealed a striking pattern of bidirectional dysregulation for many cell type-specific genes. Typically, these genes were repressed in their primary cell type, yet de-repressed in other striatal cell types. Integration with existing epigenomic and transcriptomic data suggest that partial loss-of-function of the Polycomb Repressive Complex 2 (PRC2) may underlie many of these transcriptional changes, leading to deficits in the maintenance of cell identity across virtually all cell types in the adult striatum.
The maintenance of polygenic sex determination depends on the dominance of fitness effects which are predictive of the role of sexual antagonism
Authors: Meisel Richard P. 
Published: July 2020

AbstractIn species with polygenic sex determination, multiple male- and female-determining loci on different proto-sex chromosomes segregate as polymorphisms within populations. The extent to which these polymorphisms are at stable equilibria is not yet resolved. Previous work demonstrated that polygenic sex determination is most likely to be maintained as a stable polymorphism when the proto-sex chromosomes have opposite (sexually antagonistic) fitness effects in males and females. However, these models usually consider polygenic sex determination systems with only two proto-sex chromosomes, or they do not broadly consider the dominance of the alleles under selection. To address these shortcomings, I used forward population genetic simulations to identify selection pressures that can maintain polygenic sex determination under different dominance scenarios in a system with more than two proto-sex chromosomes (modeled after the house fly). I found that overdominant fitness effects of male-determining proto-Y chromosomes are more likely to maintain polygenic sex determination than dominant, recessive, or additive fitness effects. The overdominant fitness effects that maintain polygenic sex determination tend to have proto-Y chromosomes with sexually antagonstic effects (male-beneficial and female-detrimental). In contrast, dominant fitness effects that maintain polygenic sex determination tend to have sexually antagonistic multi-chromosomal genotypes, but the individual proto-sex chromosomes do not have sexually antagonstic effects. These results demonstrate that sexual antagonism can be an emergent property of the multi-chromosome genotype without individual sexually antagonistic chromosomes. My results further illustrate how the dominance of fitness effects has consequences for both the likelihood that polygenic sex determination will be maintained as well as the role sexually antagonistic selection is expected to play in maintaining the polymorphism.
Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
Authors: Chen James , Malone Brandon , Llewellyn Eliza , Grasso Michael , Shelton Patrick M. M. , Olinares Paul Dominic B. , Maruthi Kashyap , Eng Ed , Vatandaslar Hasan , Chait Brian T. , Kapoor Tarun , Darst Seth A. , Campbell Elizabeth A. 
Published: July 2020

SUMMARYSARS-CoV-2 is the causative agent of the 2019-2020 pandemic. The SARS-CoV-2 genome is replicated-transcribed by the RNA-dependent RNA polymerase holoenzyme (subunits nsp7/nsp82/nsp12) along with a cast of accessory factors. One of these factors is the nsp13 helicase. Both the holo-RdRp and nsp13 are essential for viral replication and are targets for treating the disease COVID-19. Here we present cryo-electron microscopic structures of the SARS-CoV-2 holo-RdRp with an RNA template-product in complex with two molecules of the nsp13 helicase. The Nidovirus-order-specific N-terminal domains of each nsp13 interact with the N-terminal extension of each copy of nsp8. One nsp13 also contacts the nsp12-thumb. The structure places the nucleic acid-binding ATPase domains of the helicase directly in front of the replicating-transcribing holo-RdRp, constraining models for nsp13 function. We also observe ADP-Mg2+bound in the nsp12 N-terminal nidovirus RdRp-associated nucleotidyltransferase domain, detailing a new pocket for anti-viral therapeutic development.
Humanistic leadership in Thailand: a mix of indigenous and global aspects using a cross-cultural perspective
Authors: Vora Davina , Kainzbauer Astrid 
Published: July 2020

PurposeTo explore how leadership behavior in Thailand relates to humanistic leadership through indigenous and cross-cultural lenses.Design/methodology/approachAnalogically based and semi-structured interviews were used. The primary focus was on factors associated with expatriate success in leading Thais in a Thai context. As such, the main sample included 24 expatriates. Two local Thai leaders were also interviewed. Qualitative interviews were analyzed inductively using NVivo.FindingsFive interrelated themes emerged from the data: guiding, bridging, emotionally supporting, socializing and indirectly communicating. These themes relate to Asian holistic thinking, Thai culture and humanistic management. Evidence for humanistic leadership was found, albeit in culture-specific ways.Research limitations/implicationsResearchers may benefit from studying local, indigenous leadership practices and determining if and how they fit etic concepts such as humanistic leadership. Limitations of this study include a small sample from only one country.Practical implicationsTo be successful, leaders should engage in humanistic leadership practices that fit the Thai context. Human resource departments may wish to focus their talent recruitment, selection and development on these behaviors.Originality/valueThis paper adds to the nascent literature on humanistic leadership by providing an indigenous as well as cross-cultural lens to understanding humanistic leadership in the context of Thailand.
Cross-cultural comparison of mental health between Japanese and Dutch workers: relationships with mental health shame, self-compassion, work engagement and motivation
Authors: Kotera Yasuhiro , Van Laethem Michelle , Ohshima Remi 
Published: July 2020

PurposeThe primary purpose of this descriptive study was to compare the levels of, and relationships among mental health problems, mental health shame, self-compassion, work engagement and work motivation between workers in Japan (collectivistic and success-driven culture) and the Netherlands (individualistic and quality-oriented culture).Design/methodology/approachA cross-sectional design, where convenience samples of 165 Japanese and 160 Dutch workers completed self-report measures about mental health problems, shame, self-compassion, engagement and motivation, was used. Welch t-tests, correlation and regression analyses were conducted to compare (1) the levels of these variables, (2) relationships among these variables and (3) predictors of mental health problems, between the two groups.FindingsDutch workers had higher levels of mental health problems, work engagement and intrinsic motivation, and lower levels of shame and amotivation than Japanese workers. Mental health problems were associated with shame in both samples. Mental health problems were negatively predicted by self-compassion in Japanese, and by work engagement in Dutch employees.Originality/valueThe novelty of this study relates to exploring differences in work mental health between those two culturally contrasting countries. Our findings highlight potential cultural differences such as survey responding (Japanese acquiescent responding vs Dutch self-enhancement) and cultural emphases (Japanese shame vs Dutch quality of life). Job crafting, mindfulness and enhancing ikigai (meaningfulness in life) may be helpful to protect mental health in these workers, relating to self-compassion and work engagement. Findings from this study would be particularly useful to employers, managers and staff in human resources who work with cross-cultural workforce.
Poverty trap in Mexico, 1992-2016
Authors: Loría Eduardo 
Published: July 2020

Purpose
The paper aims to prove that between 1992 and 2016, people in poverty as a proportion of the total population has not been reduced. In particular, food poverty (FP) represented an average of 22%, despite the fact that gross domestic product (GDP) per capita and GDP, social development expenditure and food programme expenditure (both as GDP proportion) grew by 0.96%, 1.9%, 2.7% and −17.4% on an annual average, respectively.

Design/methodology/approach
There are non-linear relationships between economic growth and food poverty expenditure to reduce poverty. Three econometric models were estimated as follows: a linear model [ordinary least squares (OLS)] that addresses the capability of the economic growth to reduce FP (which detects a structural change in 2007) and three models of regime change (Markov–Switching Regression) that prove the existence of two different regimes.

Findings
The author proved that economic growth has lost the capability to reduce poverty and that there are decreasing effects of expenditure in addressing poverty since 2007. These results point out that Mexico is in a poverty trap and suggests that for the economy as for life and even more in the case of social (public) policies, more is not necessarily better than less. Therefore, the author suggests that the resources allocated in response to poverty may well have generated perverse incentives that yielded the opposite results.

Originality/value
There is no official measure of the public expenditure for poverty. Therefore, an accurate series was built to estimate the government effort and do the econometrics that proves the main hypothesis. This is another contribution.
Effects of operating conditions on cavitation induction of spiral groove liquid-film seal (SG-LFS)
Authors: Li Zhentao , Yin Xiaoli , Yue Jixiang , Liu Fuyu , Hao Muming , Ren Baojie 
Published: July 2020

PurposeThe purpose of this paper is to study the effects of operating conditions including process coefficient, lubricant viscosity and cavitation pressure on the cavitation of spiral groove liquid-film seal (SG-LFS).Design/methodology/approachA mathematical model of SG-LFS is established based on the JFO boundary and a relative density is introduced. The universal governing equation after a coordinate transformation is discretized by the FVM method and solved by the Gauss-Seidel relaxation scheme.FindingsThe results indicate that the two-dimensional size of cavitation and cavitation degree are affected significantly by the process coefficient and lubricant viscosity but the effect of cavitation pressure can be ignored.Originality/valueThe effect mechanisms of operating conditions on the cavitation of SG-LFS are studied by the JFO boundary and cavitation degree characterized by a relative density. The results presented are helpful to perfect and deeply understand the cavitation mechanism of liquid-film seal.Peer reviewThe peer review history for this article is available at:https://publons.com/publon/10.1108/ILT-03-2020-0083/
Spin-type forward motion mode based on double steering wheel parking AGV
Authors: Pan Ting , Yan Jiaqing , Zhou Shenyun , Cai Yingjie , Lu Congda 
Published: July 2020

Purpose
The purpose of this paper is to propose the situation that the existing parking automated guided vehicle (AGV) has a single walking mode, a spin forward motion mode based on a dual steering wheel driven parking AGV. In this way, the AGV can complete the 180° spin of the AGV in the process of straight forward.

Design/methodology/approach
A spin forward kinematics model of the dual steering wheel AGV is established, and a motion controller of the dual steering wheel AGV is designed based on the principle of model predictive control to complete the path following the spin forward motion mode.

Findings
Computer simulations and laboratory tests were performed on this movement mode, which showed that the operation mode was feasible. It also verified that the mode can improve the handling efficiency, and also solved the problem that the parking space beside the wall could not be set and the site utilization was improved.

Research limitations/implications
The controller should be further improved to make the operation smoother and more accurate.

Practical implications
This mode has the applicability to the indoor logistics AGVs. In addition, it can improve the handling efficiency and also solved the problem that the storage space for goods beside the wall could not be set and the site utilization was improved.

Social implications
This method can solve the problem due to the increasing number of private cars and parking spaces are hard to find. It increases the number of parking spaces and improves the utilization rate of the site. In addition, it also saves people the time to find a parking space and reduces car exhaust emissions in the process. It follows the requirements of sustainable development.

Originality/value
The studies in this paper provide AGV with more ideas on the issue of improving handling efficiency and site utilization and also solves the problem of being unable to set parking spaces when parking against the wall. In addition, this model has applicability to indoor logistics AGV and plays the same role.
Social capital for knowledge management system of the creative industry
Authors: Mayasari Yanti , Chandra Teddy 
Published: July 2020

PurposeTh purpose of this paper is to represent the role of social capital for the knowledge management system (KMS) in the kind of literature which is related to the topics in the creative industry.Design/methodology/approachThis paper uses conceptual and literature study with empirical quantitative and qualitative investigation to validate. As some literature states that KMS is an organizational process and tool for acquisition, conversion, application and protection of existing knowledge as a way to use, develop and manage it which comes from internal and external organizations.FindingsLiterature shows that for some organizations, knowledge is gained through research and development (R&D) of the internal organization. In contrast, the creative industry requires knowledge that is derived from social capital such as social environment and community. The social environment and community (social capital) will provide knowledge that is required for the existence of the creative industry in producing the creative product that may represent the social context in which the creative industry exists. This study uses a meta-analysis as a tool of analysis to classify previous research and studies regarding the roles of social capital for KMS in the creative industry that were used as the cornerstone of the research.Originality/valueStudies in the creative industry previously show that knowledge is a collaboration of tacit knowledge and explicit knowledge which is gained from various the creative classes within the industry. As a renewable resource-based industry, creativity, skill and talent are resources that are used to be commercialized to gain wealth for not only big industries but also for micro, small and medium economies (UMKM) that mostly done by communities, to create employment through the exploitation of intellectual property. Therefore, the creative industry can be meant as a knowledge-based industry that requires the application of KMS in its operation.
Money laundering via underground currency exchange networks
Authors: Teichmann Fabian Maximilian , Falker Marie-Christin 
Published: July 2020

Purpose
This paper aims to demonstrate how illicit funds are being laundered through underground currency exchange networks.

Design/methodology/approach
Sixty interviews with money launderers and compliance officers were conducted to identify methods relevant to current money laundering issues. Further, a quantitative survey of 200 compliance officers was administered.

Findings
The currency exchange method is highly suitable for money launderers with access to a criminal network. It may be used for placement or pre-placement. Evidently, the vast majority of compliance officers fail to recognize the utilization of this method in their daily business.

Research limitations/implications
Implications are based on the statements of 60 interviewees, comprising both alleged money launderers and compliance officers.

Practical implications
The study identified gaps in anti-money laundering mechanisms. The documentation of said inconsistencies aims to provide compliance officers, law enforcement agencies and legislators with useful insights into the minds of money launderers.

Originality/value
Whereas most prior literature focuses on money laundering prevention methods, how money launderers operate is not illustrated. This study comprehensively overviews the issue by interviewing not only compliance officers but also money launderers. Understanding how money launderers operate is essential to effectively prevent money laundering. In particular, compliance officers must be able to view money laundering from the criminal’s perspective to sufficiently combat the issue.
Impact of activity restrictions on risk taking of banks: does competition matter during crisis?
Authors: Noman Abu Hanifa Md. , Isa Che Ruhana , Mia Md Aslam , Sok-Gee Chan 
Published: July 2020

Purpose
This study aims to examine the impact of activity restrictions in shaping the risk-taking behaviour of banks through the channel of competition in different economic conditions.

Design/methodology/approach
The authors use a dynamic panel regression method, particularly a two-step system generalised method of moments to address the risk-taking persistence of banks and endogeneity of activity restrictions and competition with banks’ risk-taking using financial freedom and property rights as instrumental variables. Activity restrictions are computed by constructing an index based on the survey results of Barth et al. (2001, 2006, 2008 and 2013a). Competition is measured by the Panzar–Rosse H-statistic and risk-taking behaviour are measured by non-performing loan ratio and lnZ-score. In the investigation process, the authors control bank characteristics – size, efficiency, ownership and loan composition and macroeconomic factors – gross domestic product growth and inflation, and use 2,527 bank-year observations from 180 commercial banks of Association of the Southeast Asian Nations-five countries over the 1990–2014 period.

Findings
This study finds that activity restrictions exacerbate the risk-taking behaviour of the banks leading to changes in the channel of competition because of the “risk-shifting effect” of competition. The finding is robust by considering the financial crisis and alternative specifications.

Research limitations/implications
This study contributes to bank literature and policy formulation regarding the effect of activity restrictions on the risk-taking behaviour of banks, which is an issue of concern amongst bank regulators, policymakers and academics, especially in the aftermath of the 2008–2009 global financial crisis.

Practical implications
Understanding how the competition plays a role in the relationship between activity restrictions and the risk-taking of banks in different economic situations.

Originality/value
This study provides new insight into the bank literature by investigating the moderating role of competition on activity restrictions and the risk-taking behaviour of banks in a different economic environment.
The battle of superheroes: the rise of the knowledge platform strategy in the movie industry
Authors: Trabucchi Daniel , Magistretti Stefano 
Published: July 2020

Purpose
Over the past years – because of the huge impact of companies such as Airbnb and Uber – the concept of the platform became extremely popular in the business world. Still, the concept of the platform has been evolving for a long time in the management field, from internal platforms (like the famous case of Sony Walkman) to industry-wide platforms (like the case of computers with external software developers), up to transaction and two or multi-sided platforms (such as Airbnb and all the companies with a similar business model). Platforms are often considered disruptors in several industries, from accommodations to mobility to entertainment. Still, the disruptors in the creative world usually deal with the way in which they distribute content (Netflix or Spotify), rather than with the content itself. The purpose of this paper is to understand if and how platforms trategies can also be used in the creative industries.

Design/methodology/approach
This paper digs into the movie industry, studying the three most successful Superheroes sagas of the past two decades as follows: the Marvel Cinematic Universe (MCU), DC Comics and X-Men using a narrative approach. The three sagas are studied through network analysis to understand their “platform approach”.

Findings
The results show how platform strategies are relevant also in creative industries – defining the concept of “knowledge platform strategy” – and how they have a significant impact in terms of market performance. The MCU builds on a common knowledge through the various movies that enable the chance to rely on many of the platforms’ characteristics emerging from the literature.

Research limitations/implications
This research extends the concept of “platforms,” relying on the three kinds of platform defined in the managerial literature and introducing the “knowledge platform strategy” for creative industries. The main contribution is related to the extension of platforms in research fields where it has not been exploited. This opens up avenues for research both from a knowledge platform and creative industries perspectives.

Practical implications
Managers, working not only in creative industries but also in industries where a common knowledge basis may be leveraged to develop new products over time, may use the concept of “knowledge platform strategy” to rethink the new development process or knowledge management from a customer perspective.

Originality/value
This study explores a mature and relevant concept – the platforms – in a new filed, the creative industries using the case of Superheroes sagas, proposing a new perspective to explain the success of MCU while proposing a new platform strategy.