bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histocytological Study of Somatic Embryogenesis in the Tree Cinnamomum camphora L. (Lauraceae)
Authors: JING Ruyue , WANG Peilan , HUANG Zhen , LI Zhihui 
Published: December 2019

Histocytological studies were conducted on primary, secondary, and malformed embryos produced during somatic embryogenesis of Cinnamomum camphora L. to better understand its development. Exploring its callus types and structures provided a theoretical basis for clarifying the mechanism of somatic embryogenesis, which may shed light on the mechanism of zygotic embryogenesis. We used immature zygotic embryos as explants to induce somatic embryos, forming many embryogenic calli that differentiated into mature somatic embryos. Our results showed that somatic embryogenesis of C. camphora was similar to that of zygotic embryos. We have been dedifferentiated four types of callus. Compared with non-embryogenic cells, embryogenic cells had a closer arrangement, larger nucleus, thicker cytoplasm, more starch granules and easier to stain into black. Somatic embryogenesis had two pathways: direct (predominate) and indirect (rare). Embryogenic cells of C. camphora could have either an internal or external origin, the latter being primary, for which occurrence sites include epidermis and near-epidermis (little internally). Mostly arising from single cells, C. camphora follows two developmental pathways: single-cell equal as opposed to unequal, wherein both divide to form multi-cell proembryos. However, multicellular origins can occasionally occur and feature physiological isolation during somatic embryo development. This development has four embryo stages: globular, heart-shaped, torpedo, and cotyledon, with procambium cells apparent in globular embryos and late cotyledons forming “Y-shaped” vascular bundles. Secondary embryos were present in all stages, directly occurring on primary embryo’s germ and radicle end surfaces. We conclude that secondary and primary embryos of C. camphora undergo similar developmental processes. At the same time, conjoined cotyledon embryos and morphological abnormal embryos were found, with an internal origin more likely to generate abnormal embryos.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Growth Analysis of Quinoa (Chenopodium quinoa Willd.) in Response to Fertilization and Soil Tillage
Authors: KAKABOUKI Ioanna , ROUSSIS Ioannis E. , PAPASTYLIANOU Panagiota , KANATAS Panagiotis , HELA Dimitra , KATSENIOS Nikolaos , FUENTES Francisco 
Published: December 2019

Growth analysis is an appropriate method for plant response to various environmental and cultural conditions during plant life. A 2-year experiment was conducted to evaluate the effect of soil tillage and fertilization on the growth and growth parameters of quinoa crop and to determine the association between yield and growth characteristics at both the single plant and crop stand level. The experiment was laid out in a split-plot design with two replicates, two main plots [conventional (CT) and minimum tillage (MT)] and four sub-plots [fertilization treatments: untreated, inorganic fertilization of 100 (N1) and 200 kg N ha-1 (N2) and sheep manure]. The highest absolute growth rate (AGR) and crop growth rate (CGR) values were recorded between the middle of vegetative growth stage and the beginning of anthesis (50-75 DAS) under conventional tillage coupled with manure (AGR: 0.4577 g day-1, CGR: 11.44 g m-2 day-1) and with N2 treatment (AGR: 0.4521 g day-1, CGR: 11.31 g m-2 day-1). Concerning specific leaf area (SLA), the highest value (150.58 cm2 g-1) was found at 75 DAS in N2 treatment. Leaf area index (LAI) were positively affected by soil tillage and fertilization with greatest values found under conventional tillage coupled with N2 treatment (5.110 m2 m-2). The highest seed yield was observed in N2 treatment (2488 and 2388 kg ha-1 under CT and MT, respectively). As a conclusion, the cultivation under conventional tillage and the increasing levels of applied nitrogen up to 200 kg N ha-1 increases crop growth and yield.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Genetic Diversity of some Daffodil (Narcissus L. spp.) Genotypes in Turkey by Using SRAP Markers
Authors: ZEYBEKOĞLU Emrah , KAYGISIZ ASCIOGUL Tansal , ILBI Hulya , OZZAMBAK Mustafa E. 
Published: December 2019

Daffodil (Narcissus L. spp.) is one of the oldest ornamental flowers that have been cultivated from ancient times to nowadays. The species Narcissus tazetta L. and N. serotinus L. have spread out in different regions of Turkey and some other species have been naturalized. The purpose of this study was to characterize some daffodil populations that were collected from different regions of Turkey by using SRAP markers. Twenty-two daffodil populations of different species such as N. tazetta, N. serotinus, N. pseudonarcissus L., N. poeticus L. and N. jonquilla L. were used. Thirty-seven of 48 SRAP primer combinations showed scorable and polymorphic bands between samples. The similarity coefficients were used to construct a dendrogram by Unweighted Pair-Group Method with Arithmetic Averages (UPGMA) using NTSYS-PC. Cophenetic correlation between ultrametric similarities of tree and similarity matrix were high (r = 0.97). The genotypes of Narcissus species were divided into 2 main groups. Although there was less genetic variation among N. tazetta species, even collected from different regions of Turkey, N. poeticus, N. pseudonarsis, N. jonquilla and N. serotinus had more genetic divergence among them. It can be concluded that genetic variation of Narcissus species can be used for daffodil breeding programs.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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The Estimation of Nitrogen Uptake and Utilization Efficiency in Cotton by the Fertilizer-Response Model
Authors: MA Lulu , ZHANG Qiang , LIN Jiao , SU Wei , YIN Caixia , LV Xin , ZHANG Ze 
Published: December 2019

Due to the indeterminate growth habit of cotton crops, a better understanding of N status at the rational fertilizer regime is important to promote lint yield. The fertilizer-response model was employed to evaluate N status by analyzing data of shoot dry mass, N content and N concentration at different growing stages. A field study was conducted on drip-irrigated cotton plants with N fertilizer addition in total amounts of 0 (N0), 120 (N1), 240 (N2), 360 (N3) and 480 (N4) kg ha-1 in Xinjiang, China in 2016. Thirty percent of total fertilizers were applied at planting and the rest 70% were applied over six applications. The N fertilizer treatment at the accumulative rate of 70 kg ha-1 was enough to induce the N status of steady state accumulation 60 days after germination. Since 90 days the treatments that delivered the N amount between 120 and 240 kg ha-1 was deficient for cotton demand, higher rates from 360 and 480 kg ha-1 induced inherent N reserve and resulted in the highest level of yield. With regard to the practical meaning, the N fertilizer dose of 360 kg ha-1 can be used for cotton growth. The N fertilizer dose of 120 kg ha-1 can be recommended when the yield of 5,840 kg ha-1 lint can meet the goal of cotton culture.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Inhibitory Effects of Iso-α and β Hop Acids Against Pediococcus pentosaceus
Authors: MICHIU Delia , DELVIGNE Frank , MABON Nicolas , JIMBOREAN Mirela , FOGARASI Melinda , MIHAI Mihaela , TOFANĂ Maria , THONART Philippe 
Published: December 2019

The goal of the research was to assess the inhibitory effects of hop extracts, iso-α and β acids, against Pediococcus pentosaceus bacteria, during a short incubation period, both in liquid selective media (high pH values) and beer wort fermentation (low pH values) and testing if the identified iso-α acid stress changes the activity of S. cerevisiae boulardii yeast and ethanol production. Flow cytometry analysis was used for bacterial and yeast cell viability. In relation to the antibacterial activity of β-acids, a lower viability of Pediococcus pentosaceus cells was observed after a short incubation period in selective media, under iso-α acid stress. In beer wort, for a mixed culture with P. pentosaceus bacteria and S. cerevisiae boulardii yeast, under iso-α acid stress conditions at pH 4.0-5.0, Pediococcus pentosaceus exhibited lower cell viability (20.7%) than in selective media (61.4%). Regarding iso-α hop acid on S. cerevisiae boulardii yeast, the results showed that iso-α does not change the S. cerevisiae activity but prevents the culture from being contaminated by Pediococcus pentosaceus. The results highlighted reliable inhibitory effects of iso-α and β-acids against P. pentosaceus, both at pH 6.0-7.0 and pH 4.0-5.0, which open the possibility of hops being used as a supplement to prevent beverage contamination with spoilage microorganisms.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Bioremediation of Phenol by Mutated and Immobilized Aspergillus and Penicillium Species
Authors: IBRAHIM Amany G. , AL-GHAMDI Lujin  S. 
Published: December 2019

Phenol and its chemical derivatives are essential for production of polycarbonates epoxies, bakelite, nylon, detergents, herbicides, and numerous pharmaceutical drugs. In order to increase the biodegradation of phenol by fungi, fungal strains (Aspergillus niger, Penicillium griseofulvum and Aspergillus terreus), were isolated from different contaminated sites in Saudi Arabia such as Jeddah Governate, the second industrial city of Jeddah, some garbage collection places, gas stations and Red Sea), then screened for phenol degradation. For the first time in Saudi Arabia, biodegradation of phenol by fungi is improved by mutation as well as immobilization of fungi above calcium alginate. The isolated fungal strains (Aspergillus niger, Penicillium griseofulvum and Aspergillus terreus), were mutated physically (UV) and chemically (Ethidium bromide), also immobilized in alginate beads and its phenol degradation efficiency was observed. The degradation was increased many fold after immobilization, but after mutation some mutants appeared highly degradation rate for the phenol such as Aspergillus niger, and Penicillium griseofulvum but Aspergillus terreus appeared highly degradation rate for the phenol only after exposure to UV for 5 and 10 mins only than the wild strains. In addition, phenol degradation was increased with increase the fungal disk size of the tested strains.
Introduction Pages
Authors: SESTRAS Radu E. 
Published: December 2019

Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 4, Volume 11, 2019: The papers published in this issue represent interesting novelties in different topics of life science. Among the exciting researches, we invite readers to find news about: anticancer activity of water and methanol extracts of Hypericum scabrum L. on different cancer cell lines; curcumin alleviates potassium bromate-induced hepatic damage by repressing CRP Induction through TNF-α and IL-1β and by suppressing oxidative stress; evaluation of leukocyte mobilization and platelet aggregatory effects of ciprofloxacin, lincomycin and erythromycin in Wistar albino rats; ameliorative effect of aqueous leaf extract of Moringa oleifera on reproductive function following cadmium chloride induced oxidative stress in male Wistar rats; acute and sub-acute toxicity of Cola millenii leaf and stem bark extracts in albino rats; the effects of using synthetic and natural color foods on lipid profile and liver function in rats; phylogenetic analysis and in silico characterization of cytochrome P450 1A (Cyp1A) protein from the African catfish, Clarias gariepinus; growth performance indices of New Zealand white rabbits administered Panax ginseng extracts; the clinicopathological evaluations of the efficacy of incorporation of amantadine hydrochloride in the treatment of West African dwarf goats experimentally infected with peste des petits ruminants virus; in vitro plant regeneration from mature seed explants of Withania somnifera, an important, rare and endangered medicinal plant.
Dioxins and furans’ behavior in the process of zinc and lead removing from EAF dust
Authors: Simonyan L. M. , Demidova N. V. 
Published: December 2019

The use of galvanized scrap as a charge material for electric steel-smelting production leads to formation of metallurgical dust suitable for extraction of non-ferrous metals. Chlorine and organic compounds content in metallurgical charge can lead to dioxins and furans formation in the process of electric smelting with their subsequent sedimentation on EAF dust. In the previous study we determined dioxins and furans content in dust at the level of 474 ng/kg of EAF dust. The methodology for conducting an experiment in a muﬄe furnace at temperatures of 300, 600, 900 and 1150 °Cwas developed for the study of dioxins and furans’ behavior during dust heating. Investigation of EAF dust chemical composition before and after the experiment made it possible to establish that desorption of dioxins and furans occurs during heating in the temperature range of 300 – 900 °C. In parallel with dioxins and furans’ desorption some chemical compounds evaporate indirectly and it is determined by calculation of changes in the content of C, Na, Cl, K, Pb, Zn in the sample. In the studied temperature range, the content of C, Na, Cl decreases to zero; K content is reduced by 81 %; Pb content is reduced by 83.5 %. Reduction of Zn content does not exceed 5 %. Change in content of the remaining components is insignificant. The obtained data confirm the predominant presence of chlorine in inorganic compounds in forms of NaCl and KCl, along with a slight presence in forms of ZnCl, PbCl, and PbCl2 . The study revealed the need of consideration of dioxins and furans’ presence during development of technologies aimed at metallurgical dust processing. It is proposed to perform high-temperature processing of dust (>850 °С) with the subsequent irrigation of exhaust gases with lime milk. The most rational ways to decrease dioxins and furans’ content in EAF dust are conducting afterburning of exhaust gases, followed by rapid cooling in order to avoid secondary synthesis of ecotoxicants or reducing the amount of chlorine-containing and conventional materials during pretreatment of metals.
Two-phase state of the melts of Fe – Cu system during their cooling in a viscometer
Authors: Filonov M. R. , Sanin V. V. , Anikin Yu. A. , Kostitsyna E. V. , Vidineev S. N. 
Published: December 2019

Alloys Fe – Cu can be characterized as a system with immiscible components (IC). This statement is based on a weak mutual solubility in the solid state. In addition, with low carbon content, the Fe – Cu system is also stratified in the liquid state [1]. Alloys with IC have a simple phase composition of almost pure components. This determines a significant practical interest in these alloys. Certain successes have been achieved in the technology of manufacturing damping alloys of the Fe – Cu– Pb system. With optimally selected technology for their preparation, it is possible to obtain the final product combining the properties of pure alloy components in the proportion required for practical application. For example, in Fe – Cu alloys, diamagnetic copper has high electrical conductivity and thermal conductivity, and ferromagnetic iron has enhanced strength characteristics compared to copper. When structure of the alloy is organized in a certain way, it is possible to obtain either a final product with high electrical conductivity and thermal conductivity of copper, enhanced strength properties of iron, or – a hard magnetic material with ductility of copper. The ironcopper system alloys were considered in [2 – 13], in which the main attention was paid to structural studies and measurements of service properties. At the same time, the dynamics of macroand microstructure formation of alloys were not analyzed. In the present work, it was the dynamics of macrostructure formation of the solid phase enriched with iron in process of crystallization of the melt during its cooling that was studied using high-temperature viscometry. Considering the final influence of the melt cooling rate on the size and morphology of crystallizing inclusions, and a significant amount of the two-phase area, special attention was paid to thermophysical analysis of the measurement mode. Analysis of reliability of the results obtained was made by the method of viscosity measuring. Phase state of the melts of Fe – Cu system was investigated during cooling by changing the damping factor. The analysis of thermophysical processes occurring in the measurement of the damping factor was carried out. It has been established that the process of measuring the damping decrement takes place under quasi-equilibrium conditions and the cooling rate is close to zero. There are no temperature gradients, both in radius and in height. For compositions Fe50Cu50 , Fe40Cu60 , Fe30Cu70 the dynamics of precipitation of the solid phase were determined.
5E Öğrenme Modeline Dayalı Bilgisayar Animasyonları Destekli Öğretim Materyali Tasarlama: “Tepkimelerde Hız ve Denge” Ünitesi Örneği
Authors: CEYLAN Nazan , SEÇKEN Nilgün 
Published: December 2019

Kimyasal denge ünitesindeki kavramların, soyut yapıları ve öğrencilerin bu kavramların sembolik, makro ve mikro boyutları arasında ilişki kuramamasından dolayı geleneksel yaklaşımlar ile kazandırılmasında zorluklar yaşanmaktadır. Diğer bir sorun ise bu gibi soyut kavramların öğretiminde kullanılabilecek uygun öğretim materyallerinin sınırlılığıdır. Yapılandırmacı yaklaşım öğrencilerin günlük yaşam deneyimlerine dayanmaktadır. Soyut kavramların öğretiminde karşılaşılan sorunların çözümünde; bilgisayar teknolojisinin kullanımı, günlük hayat örnekleri ile zenginleştirilmiş, soyut kavramları somutlaştıracak ve öğrencilerin öğrenmelerini kolaylaştıracak bilgisayar destekli öğretim (BDÖ) materyallerinin geliştirilmesi kavramların somutlaştırılmasına katkı sağlayacaktır. Bu çalışmada ortaöğretim kurumlarında kimya dersi kapsamında 11. sınıf öğretim programında yer alan “Tepkimelerde Hız ve Denge” konusunda 5E öğrenme modeli esas alınarak animasyon ve eğitsel bilgisayar oyunu destekli öğretim materyali geliştirmek ve geliştirilen bu materyalin pilot uygulamasını yaparak son şeklini vermek amaçlanmıştır. 5E öğrenme modeli esas alınarak geliştirilen materyal ADDIE modeli kullanılarak hazırlanmıştır. Yazılımın güncellenmesi için 12 lise öğrencisi ile pilot uygulama yapılmıştır. Pilot uygulamanın sonuçları ve uzmanların önerisi ile yazılıma son şekli verilmiştir.
Риторика агентов социального контроля в отношении профилактики противоправного поведения молодежи
Authors: Шипунова Татьяна Владимировна 
Published: December 2019

Зарубежные исследования свидетельствуют о низкой эффективности профилактического социального контроля. В России подтверждением тому являются негативные тенденции развития противоправного поведения, которые, в первую очередь, выражаются в его росте, омоложении и феминизации. Изучение причин неэффективности профилактики можно проводить не только на основе рассмотрения негативных результатов профилактического воздействия, но и путем анализа оценок, даваемых агентами социального контроля. Для этого предлагается осмыслить предмет в рамках социального конструктивизма, который рассматривает социальный контроль как коммуникацию в социальных взаимодействиях, предполагающую передачу некоторой информации, идей, конструктов социальной реальности посредством знаков и символов. Для изучения коммуникации использован метод деконструкции риторики агентов профилактики (работников полиции, школы, соцзащиты). Автор обрисовывает теоретические рамки изучения риторик, представляет результаты анализа транскриптов фокусированного интервью с экспертами. В качестве экспертов выступили представители полиции, школы, государственных и негосударственных учреждений социальной защиты населения, занимающихся профилактикой противоправного поведения. Деконструированы риторики, касающиеся взаимодействия агентов профилактической работы с молодежью. Исследование показало, что состояние этой деятельности описывается агентами контроля либо в идиоме опасности, либо с использованием лейтмотивов бедствия. У представителей полиции и школы субъективное восприятие угрозы связано с неэффективной деятельностью соответствующих учреждений и недостаточной заинтересованностью родителей. Эксперты использовали также конструкты риторики наделения правом, которые ассоциированы с расширением полномочий самих агентов контроля. Представители государственных и негосударственных организаций социальной защиты при описании состояния профилактической деятельности чаще используют лейтмотивы бедствия. Они связывают угрозы с недостаточным вниманием государства к проблеме профилактики противоправного поведения молодежи. Это выражается в скудной финансовой поддержке, непродуманности законов, сокращении сотрудников, неоправданно жестком контроле деятельности агентов профилактической работы.