bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Efficacy of OnabotulinumtoxinA Treatment in Patients With Comorbid Migraine and Depression: A Meta-analysis
Authors: Affatato Oreste , Moulin Thiago C. , Pisanu Claudia , Babasieva Victoria S. , Russo Marco , Aydinlar Elif I. , Torelli Paola , Chubarev Vladimir N. , Tarasov Vadim V. , Schiöth Helgi B. , Mwinyi Jessica 
Published: December 2020

Abstract
Background: Migraine and depression are highly prevalent and partly overlapping disorders that cause strong limitations in daily life. Patients tend to respond poorly to the therapies available for these diseases. OnabotulinumtoxinA has been proven to be an effective treatment for both migraine and depression. While many studies have addressed the effect of onabotulinumtoxinA in migraine or depression separately, a growing body of evidences suggest benecial effects also for patients comorbid with migraine and depression. The current meta-analysis systematically investigates to what extent onabotulinumtoxinA is efficient in migraineurs with depression.Methods: A systematic literature search was performed based on PubMed, Scopus and Web of Science from the earliest date till October 30th, 2020. Mean, standard deviation (SD) and sample size have been used to evaluate improvement in depressive symptoms and migraine using random-effects empirical Bayes model. Results: Our search retrieved 259 studies, eight of which met inclusion criteria. OnabotulinumtoxinA injections administered to patients with both chronic migraine and major depressive disorder led to mean reduction of -8.94 points (CI: [-10.04, -7.84], p < 0:01) in the BDI scale, of -5.90 points (CI: [-9.92, -1.88], p < 0:01) in the BDI-II scale and of -6.19 points (CI: [-9.52, -2.86], p < 0:01) in the PHQ-9 scale, when evaluating depressive symptoms. In the case of the migraine-related symptoms, we found mean reductions of -4.10 (CI: [-7.31, -0.89], p = 0.01) points in the HIT6 scale, -32.05 (CI: [-55.96, -8.14], p = 0.01) in the MIDAS scale, -1.7 (CI: [-3.27, -0.13], p = 0.03) points in the VAS scale and of -6.27 (CI: [-8.48, -4.07], p < 0:01) migraine episodes per month. Comorbid patients showed slightly better improvements in BDI, HIT6 scores and migraine frequency compared to monomorbid patients. The latter group manifested better results in MIDAS and VAS scores. Conclusion: Treatment with onabotulinumtoxinA leads to a signicant reduction of disease severity of both chronic migraine and major depressive disorder in patients comorbid with both diseases. Comparative analyses suggest an equivalent strong effect in monomorbid and comorbid patients, with benecial effects specically seen for certain migraine features.
The Process of Adapting High School-based Public Health Interventions for Use in Vocational High School Settings - The Case of the Young and Active Intervention
Authors: Jensen Marie Pil , Brautsch Louise Ayoe Sparvath , Rosing Johanne Aviaja , Duus Katrine Sidenius , Jørgensen Andreas , Krølner Rikke Fredenslund 
Published: December 2020

Abstract
Objective: The Healthy High school study aimed to promote wellbeing at high schools in Denmark. While the study was still running, the funding body encouraged the project group to test the applicability of one of the high school-based intervention components, i.e. the Young and Active (Y&A) intervention component, to a Vocational Education and Training (VET) school setting. Y&A comprised a peer-led innovation workshop aimed at inspiring first-year students to develop and implement activities at school which promote sense of community and movement during the school day. Students could apply for a start-up grant to establish new activities and facilities. This research note reports on the systematic process of adapting this intervention component to a VET school setting: 1) needs assessment, 2) preliminary adaptation design, 3) preliminary adaptation testing, and 4) adaptation refinement. Results: Based on the needs assessment, the following adaptions were made to the original intervention component; 1) A narrowed objective and focus i.e. promoting active breaks during the school day, 2) The addition of activity props to support the implementation of active breaks, and 3) Changes to the target group. As part of the further adaptation process, this preliminarily adapted version will be pilot-tested among students at VET schools using mixed methods.
Estimating the Distribution of Viral Taxa in Next-generation Sequencing data using Artificial Neural Networks
Authors: Kohls Moritz , Kircher Magdalena , Krepel Jessica , Liebig Pamela , Jung Klaus 
Published: December 2020

Abstract
Background: Estimating the taxonomic composition of viral sequences in a biological sample processed by next-generation sequencing is an important step for comparative metagenomics. For that purpose, sequencing reads are usually classified by mapping them against a database of known viral reference genomes. This fails, however, to classify reads from novel viruses and quasispecies whose reference sequences are not yet available in public databases. Methods: In order to circumvent the problem of a mapping approach with unknown viruses, the feasibility and performance of neural networks to classify sequencing reads to taxonomic classes is studied. For that purpose, taxonomy and genome data from the NCBI database are used to sample artificial reads from known viruses with known taxonomic attribution. Based on these training data, artificial neural networks are fitted and applied to classify single viral read sequences to di erent taxa. Model building includes di erent input features derived from artificial read sequences as possible predictors which are chosen by a feature selection method. Training, validation and test data are computed from these input features. To summarise classification results, a generalised confusion matrix is proposed which lists all possible misclassification combination frequencies. Two new formulas to statistically estimate taxa frequencies are introduced for studying the overall viral composition.Results: We found that the best taxonomic level supported by the NCBI database is that of viral orders. Prediction accuracy of the fitted models is evaluated on test data and classification results are summarised in a confusion matrix, from which diagnostic measures such as sensitivity and specificity as well as positive and negative predictive values are calculated. The prediction accuracy of the artificial neural net is considerably higher than for random classification and posterior estimation of taxa frequencies is closer to the true distribution in the training data than simple classification or mapping results. Conclusions: Neural networks are helpful to classify sequencing reads into viral orders and can be used to complement the results of mapping approaches. The machine learning approach is not limited to already known viruses. In addition, statistical estimations of taxa frequencies can be used for subsequent comparative metagenomics.
Mediation of The Salicylic Acid Pathway by ROS1 in Response to Abiotic Stresses
Authors: Yang Liping , Lang Chenjing , Wu Yanju , Meng Dawei , Yang Tianbo , Jin Taicheng , Zhou Xiaofu 
Published: December 2020

Abstract
Background: DNA methylation plays an important role in the growth and development of plants in response to various abiotic stresses. Salicylic acid (SA) is an important signaling molecule that is synthesized by plants and induces the expression of defense genes. Results: In this paper, we investigated the molecular mechanisms by which an upstream regulator (ACD6) in the SA pathway, an ABA pathway-related gene (ACO3), and a stress resistance gene (GSTF14) were induced by various abiotic stresses. The results demonstrated that abiotic stresses, including drought, cold, and salt stresses, induced the demethylation of the repeats in the promoters of ACD6, ACO3, and GSTF14 and transcriptionally activated their expression. Furthermore, our results revealed that ROS1-mediated DNA demethylation plays an important role in the process of transcriptional activation of ACD6 and GSTF14 when Arabidopsis plants were under cold stress.Conclusions: Our results confirmed that ROS1 plays an important role in the process of defense genes in the SA pathway and stress resistance gene GSTF14 in response to abiotic stresses.
Systematic Review Protocol. Near Infra-Red Indocyanine Green Fluorescent Cholangiography Versus Intraoperative Cholangiography to Improve Safety in Laparoscopic Cholecystectomy for Gallstone Disease – A Systematic Review Protocol.
Authors: Boira Mar Achalandabaso , Memba Robert , Bashir Yasir , Pavel Mihai Calin , Llacer Erik , Estalella Laia , Julià Elisabeth , Conlon Kevin , Jorba Rosa 
Published: December 2020

Abstract
BackgroundLaparoscopic cholecystectomy has become the standard surgical approach in the treatment of cholelithiasis. The rate of bile duct injury increased significantly during the initial period of its implementation. Diverse surgical techniques and different imaging modalities have been described to provide enhanced views of the biliary anatomy and potentially prevent or early detect bile duct injuries. Both x-ray intraoperative cholangiography (IOC) and Near infra-red indocyanine green fluorescent cholangiography (NIR-ICG) have been described as safe and feasible techniques to assess biliary anatomy. The aim of this systematic review is to evaluate if NIR-ICG can visualise extrahepatic biliary anatomy more efficiently and safer than IOC in minimally invasive cholecystectomy for gallstone disease.MethodsLiterature search will be performed via Medline (PubMed), Embase, Scopus, the Cochrane Central Register of Controlled Trials and web of science. All randomised controlled clinical trials and prospective non-randomised controlled trials which report on comparison of NIR-ICG versus IOC will be included. All patients over 18 years old who require elective or urgent minimally invasive cholecystectomy (undergoing NIR-ICG during this procedure) due to gallstone disease both acute and chronic will be included. Primary outcomes will be ability to visualise extrahepatic biliary anatomy and time to obtain relevant images of the biliary tree.DiscussionOur research expects to give the reader a clear sense of the needed focus of future research and remaining uncertainties in the field. Understanding the benefits of this technique is critical to ensuring policymakers can make informed decisions as to where preventive efforts should be focused regarding specific imaging techniques. If ICG is proven to be faster and non-invasive, routine use could be encouraged and wide implementation could be a reality. Systematic review registrationIn accordance with the guidelines, our systematic review protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO), registration number CRD42020177991.
Adjuvant therapy for retroperitoneal sarcoma: a meta-analysis
Authors: Li Xiangji , Wu Tong , Xiao Mengmeng , Wu Shanshan , Min Li , Luo Chenghua 
Published: December 2020

Abstract
Background

Adjuvant therapy is a promising treatment to improve the prognosis of cancer patients, however, the evidence base driving recommendations for adjuvant radiotherapy (ART) or chemotherapy (ACT) in retroperitoneal sarcomas (RPS) primarily hinges on observational data. The aim of this study was to evaluate the effectiveness of adjuvant therapy in the management of RPS by pooling analysis.
Methods

We searched PubMed, Web of Science, Embase, ASCO Abstracts, and Cochrane Library for comparative studies (until January 2020) comparing adjuvant therapy versus surgery alone. Data on the following endpoints were evaluated: overall survival (OS), local recurrence (LR), recurrence-free survival (RFS), and metastasis-free survival (MFS). Data were summarized as hazard ratios (HR) with 95% confidence intervals (95%CI). Risk of bias of studies was assessed with Begg’s and Egger’s tests.
Results

A total of 15 trials were eligible, including 9076 adjuvant therapy and 20830 surgery alone cases (20 studies for OS, 6 studies for RFS, 2 studies for LR, and 2 studies for MFS). Meta-analysis showed that ART was associated with distinct advantages as compared to surgery alone, including a longer OS (HR = 0.80, P < 0.0001), a longer RFS (HR = 0.61, P = 0.0002), and a lower LR (HR = 0.31, P = 0.005). However, this meta-analysis failed to demonstrate a benefit of ACT for RPS, including OS (HR = 1.11, P = 0.19), RFS (HR = 1.30, P = 0.09) and MFS (HR = 0.69, P = 0.09). in the sensitivity analysis, ACT was associated with a worse OS (HR = 1.19, P = 0.0002). No evidence of publication bias was observed.
Conclusions

Overall, the quality of the evidence was moderate for most outcomes. The evidence supports that ART achieved a generally better outcome as compared to surgery alone.
Honeybee Genetics Shape the Strain-Level Structure of Gut Microbiota in Social Transmission
Authors: Wu Jiaqiang , Lang Haoyu , Mu Xiaohuan , Zhang Zijing , Su Qinzhi , Hu Xiaosong , Zheng Hao 
Published: December 2020

Abstract
Background : Honeybee gut microbiota transmitted via social interactions are beneficial to the host health. Although the microbial community is relatively stable, individual variations and high strain-level diversity have been detected across honeybees. Although the bee gut microbiota structure is influenced by environmental factors, the heritability of the gut members and the contribution of the host genetics remains elusive. Considering bees within a colony are not readily genetically identical due to the polyandry of queen, we hypothesize that the microbiota structure can be shaped by host genetics. Results : We used shotgun metagenomics to simultaneously profile the microbiota and host genotypes of individuals from hives of four different subspecies. Gut composition is more distant between genetically different bees at both phylotype- and “sequence-discrete population”-level. We then performed a successive passaging experiment within colonies of hybrid bees generated by artificial insemination, which revealed that the microbial composition dramatically shifts across batches of bees during the social transmission. Specifically, different strains from the phylotype of Snodgrassella alvi are preferentially selected by genetically varied hosts, and strains from different hosts show a remarkably biased distribution of single-nucleotide polymorphism in the Type IV pili loci. A genome-wide association analysis identified that the relative abundance of a cluster of Bifidobacterium strains is associated with the host glutamate receptor gene that is specifically expressed in the bee brain. Finally, mono-colonization of Bifidobacterium with a specific polysaccharide utilization locus impacts the expression and alternative splicing of the gluR-B gene, which is associated with an altered circulating metabolomic profile. Conclusions : Our results indicated that host genetics influence the bee gut composition, and suggest a gut-brain connection implicated in the gut bacterial strain preference. Honeybees have been used extensively as a model organism for social behaviors, genetics, and gut microbiome. Further identification of host genetic function as shaping force of microbial structure will advance our understanding of the host-microbe interactions.
Association Between Salivary Carbonic Anhydrase 6 Levels and Early Childhood Caries in Children With Chronic Bronchial Asthma
Authors: Kormi Essa Ahmed , Pani Sharat Chandra , AlNatsha Reem 
Published: December 2020

Abstract
Background and Aim: The aim of this study was to compare the level of a salivary marker (carbonic anhydrase 6 isoenzyme (CA6)) between children with and without bronchial asthma (BA) with dental caries to age and gender-matched non-asthmatic children with a similar caries profile. Materials and Methods: Saliva samples were collected from 80 children aged 6 to 8 years.The children were divided into four groups according to the presence of BA and caries: 1) high caries, presence of BA, 2) absence of caries, presence of BA, 3) high caries, absence of BA , 4) absence of caries, absence of BA . Differences in CA6 were compared among the groups using a One-Way ANOVA and Scheffe’s post hoc test. Separate regression models were developed to evaluate the impact of oral hygiene, dental caries and salivary pH on CA6 levels in children with and without BA. Results: Children with dental caries had a significantly higher CA6 level than their caries free healthy controls. Children with BA and dental caries had a significantly lower CA6 level than their caries free counterparts. The linear regression model showed dental caries had a significant inverse association with CA6 expression in children with BA but not in healthy children.Conclusion: CA6 may serve as a protective mechanism against dental caries in children with bronchial asthma.
Dietary Acid Load and Its Interaction With IGF1 and IL6 Polymorphisms on Metabolic Traits Among Postmenopausal Women
Authors: Lim Sook Yee , (0000-0002-3853-736X) Yoke Mun Chan , Ramachandran Vasudevan , Shariff Zalilah Mohd , Chin Yit Siew , Arumugam Manohar 
Published: December 2020

Abstract
Background: Despite considerable work has been done to understand the underlying disease mechanisms by elucidating its genetic etiology, still the pathophysiology of the metabolic syndrome remains unclear. While numerous studies have shown that Insulin growth factors-1 (IGF1), Interleukin-6 (IL-6) gene polymorphisms and high dietary acid load (DAL) were related to undesirable profile of metabolic traits; it remains controversial whether DAL is associated with genetic polymorphisms or risk of metabolic traits. Thus, the objective of this study was to explore the effects of DAL, IGF1 and IL6 gene polymorphisms and their potential diet-gene interactions on metabolic traits. Methods: A total of 211 community-dwelling postmenopausal women were recruited. DAL was estimated using potential renal acid load (PRAL). Blood was drawn for biochemical parameters and DNA was extracted and Agena® MassARRAY was used for genotyping analysis to identify the signaling of IGF1 (rs35767, rs7136446) and IL6 (rs1800796) polymorphisms. Interactions between diet and genetic polymorphisms were assessed using hierarchical multiple linear regression analysis.Results: Mean age of respondents was 67 ± 6.7 years. A total of 68.2%, 59.2%, 49.3%, 23.2%, 23.2% and 10.9% had elevated systolic blood pressure (SBP), hyperglycemia, excessive waist circumference (WC), diastolic blood pressure (DBP), triglycerides (TG) and low high-density lipoprotein (HDL), respectively. DAL was positively associated with FBG (β = 0.147, p < 0.05) and there was significant interaction effect between DAL and IL6 with SBP (β = 0.19, p = 0.041). On the other hand, there were no significant main effects of DAL and single nucleotide polymorphisms (SNPs) (rs35767, rs7136446 and rs1800796) on SBP, DBP, WC, TG or HDL and cholesterol ratio. There were also no significant interaction effects between DAL and IGF1 SNPs (rs35767 and rs7136446) on SBP, DBP and FBG, TG, HDL and cholesterol ratio. Conclusion: These findings did not support the interaction effects between DAL and IGF1 and IL6 SNPs (s35767, rs7136446 and rs1800796) on metabolic traits, except for SBP. Besides, higher DAL was associated with higher FBG, allowing us to postulate high DAL is a potential risk factor for diabetes. Future research delineating foods that contribute to high DAL, and how IL6 gene polymorphism influences the association with DAL on hypertension are warranted.
Value of Echocardiography Combined with Lung Ultrasound in The Diagnosis and Treatment of Coronavirus Disease 2019 (COVID-19) Pneumonia
Authors: Han Jing , Yang Xi , Xu Wei , Jin Ronghua , Liu Weiyuan , Meng Sha , Li Jin , Zheng Ying , Li Haowen , Meng Fankun 
Published: December 2020

Abstract
Purpose: To investigate relationships between echocardiography results and lung ultrasound score (LUS) in COVID-19 pneumonia patients, and the value of their combined application for evaluating COVID-19 pneumonia in diagnosis and treatment.Methods: Hospitalized patients underwent lung ultrasound and echocardiography daily. Patients with tricuspid regurgitation within 3 days after admission were collected, and the correlations and differences between pulmonary artery pressures and LUS on days 3, 8, and 13 were compared. The inner diameter of the pulmonary artery root and the sizes of the atria and ventricles were observed.Results: Pulmonary artery pressure within 3 days was positively correlated with LUS (r = 0.448, p = 0.003; r = 0.738, p = 0.000; r = 0.325, p = 0.036). On day 8 the values of both were higher than the corresponding values on days 3 and 13 (p < 0.01). On day 8 the positive rate for increased pulmonary artery pressure was 92.9%(39/42), the positive rate for increased LUS was 90.5%(38/42), and the combined positive rate for the two was 97.6%(41/42). On day 8 the inner diameters of the right atrium, right ventricle, and pulmonary artery differed significantly from the corresponding values on days 3 and 13 (p < 0.05).Conclusions: Pulmonary arterial pressure is positively correlated with LUS. The two should be combined for more informative assessment of the status of recovery from COVID-19 pneumonia.
A preliminary study of an ultrasound-based method for measuring talar displacement during the anterior drawer stress test using a Telos device
Authors: Tsutsumi Kaori , Nakaya Utayo , Koshino Yuta , Tateno Mari , Matsumoto Kazuhisa , Tanaka Mai , Yokoyama Mika , Horie Tatsunori , Samukawa Mina , Kamishima Tamotsu , Tohyama Harukazu 
Published: December 2020

Abstract
Background

Conventionally, X-ray examination has been performed to quantify the instability of the ankle joint. The aim of this study was to evaluate the reliability and validity of a new ultrasound-based examination for the quantitative detection of ankle instability, and whether the present examination could replace the conventional X-ray test.
Methods

Thirteen adults were recruited for this validation study and were divided into two groups: a control group (eight adults, 16 feet) and an ankle instability (AI) group (five adults, eight feet). The control group had Cumberland Ankle Instability Tool (CAIT) scores ≥ 25 points, and the AI group had CAIT scores < 25 points. Anterior drawer stress tests were performed using a Telos device with ultrasound (0–150 N) and X-ray (0, 120, and 150 N). The stress ultrasound was performed by two examiners, and changes in the anterior talar displacement were measured by two measurers. The inter-examiner and inter-measurer reliabilities were calculated in the control group, and correlations between stress ultrasound and conventional stress X-ray imaging results were examined at 120 and 150 N in both the control and AI groups.
Results

Ultrasound imaging detected changes in the anterior talar displacement under loads greater than 70 N. The inter-examiner and inter-measurer reliability of the ultrasound-based method were moderate and excellent, respectively, at 150 N. The tibiotalar distances observed with X-ray and ultrasound imaging at 120 N were highly correlated in the AI group.
Conclusion

The ultrasound-based method was comparable to the conventional X-ray method and represents a novel radiation-free method for the detection of ankle instability. This method has the potential for clinical application to quantitatively evaluate mechanical instability.