bluuebunny/crossref_metadata_2025_split huggingface dataset:
Learn EVAR sizing from scratch: The results of a one-day intensive course in EVAR sizing and stent graft selection for vascular trainees
Authors: Strøm Michael , Rasmussen Jonathan Lawaetz , Nayahangan Leizl Joy , de la Motte Louise , Vogt Katja , Konge Lars , Eiberg Jonas 
Published: April 2020

Background and aim Recognition of structured training in endovascular aortic repair (EVAR) for vascular trainees is increasing. Nevertheless, how trainees can achieve sufficient skills in EVAR sizing and graft selection is sparsely described. The aim of this study was to investigate the effect of systematic training in basic EVAR sizing and graft selection on vascular surgery trainees using a validated assessment tool. Methods Sixteen vascular surgery trainees were included in an intensive 6-h hands-on workshop in aortic sizing and stent graft selection for EVAR with a trainer-to-trainee ratio of 1:2. After 1-h lecture, participants did 5 h of supervised training on increasingly complex cases. Finally, the participants were tested using a validated assessment tool. Results All participants were able to size the test-case and select a stent graft combination in 24:35 (13:30–48:20) min (median and range). The participants’ overall test scores (lower is better) were in median 17.9 (11.9–28.4). This did not differ from the scores of experienced EVAR operators 14.7 (11.7–25.2) (<200 EVAR’s) ( p = .32) but was inferior to the score of EVAR experts 11.2 (9.8 –18.7) (≥200 EVAR’s) ( p = .01). The sub-score for anatomical measurements was 10.6 (3.9–18.8) and comparable with the experienced group 9.7 (8.1–12.8) ( p = .83) but inferior to the expert operators 6.5 (5.2–10.2) ( p = .04). The sub-score for stent graft selection was 7.5 (4.9–14.1) and comparable with experienced operators scoring 4.5 (3.6–12.3) ( p = .09) but inferior to the expert operators score of 5.0 (3.6–8.4) ( p = .01). Conclusion This study presents the results of a standardised one-day basic EVAR sizing and graft selection workshop. Vascular surgery trainees with no prior EVAR experience learned to size and select stent grafts for a simple infra-renal AAA on par with experienced EVAR operators.
Review and Critique on the New Higher Education Policy Promoting “The First-Class Major Programs” in China
Authors: Lu Xiaodong 
Published: April 2020

Purpose: After the concepts of first-class universities, first-class research disciplines, and first-class undergraduate education, China’s Ministry of Education (MOE) proposed a new concept of first-class undergraduate major programs and also proposed policy initiatives for relevant selection and funding. This article provides an in-depth analysis of the motives, historical background, policy texts, core concepts, and unintended consequences of the policy and proposes six criticisms. Design/Approach/Methods: This education policy analysis article uses two research methods. The first is a content analysis that focuses on policy text. The second is comparative and historical research that focuses on the history of the undergraduate education organization model in China and the background of the four first-class concepts. Findings: This article argues that the first-class major programs in undergraduate education include provincial-level points of these programs, which stealthily narrowed the scope for comparison from potentially the whole world to that of provinces. The policy also essentially strengthened the traditional concept of zhuanye as a physical unit and caused unintended consequences in its attempts to cultivate innovative talents and to adjust the zhuanye structure. Originality/Value: This article addresses a new education policy in China and its unintended consequences.
Do Outcomes of Arthroscopic Subscapularis Tendon Repairs Depend on Rotator Cuff Fatty Infiltration?
Authors: Monroe Emily J. , Flores Sergio E. , Zhang Alan L. , Feeley Brian T. , Lansdown Drew A. , Ma C. Benjamin 
Published: April 2020

Background: Rotator cuff fatty infiltration has been correlated with poorer radiographic and clinical outcomes in supraspinatus and infraspinatus tendon repairs, but this has not been well-studied in subscapularis tendon repairs. Purpose: To evaluate the influence of preoperative rotator cuff fatty infiltration on postoperative outcomes for patients undergoing arthroscopic subscapularis tendon repair. Study Design: Cohort study; Level of evidence, 3. Methods: Patients who underwent arthroscopic subscapularis repair between 2010 and 2016 were retrospectively identified, and demographic data and surgical findings were recorded. The extent of fatty infiltration was determined on preoperative magnetic resonance imaging by the Fuchs modification of the Goutallier classification. At the most recent follow-up, patients completed the Patient-Reported Outcomes Measurement Information System for Upper Extremity (PROMIS-UE) computer adaptive test and a postoperative visual analog scale for pain. The distribution of fatty infiltration was compared between patients undergoing subscapularis tendon repair versus subscapularis tendon repair combined with a posterior cuff repair. Outcomes were compared for patients using Goutallier grade 0-1 versus grade ≥2 changes in each rotator cuff muscle. Multivariate linear regression analysis was performed to evaluate the influence of muscle quality, as well as demographic factors, on PROMIS-UE scores. Significance was defined as P < .05. Results: There were 140 shoulders included (mean age, 61.8 years; 42.1% female; mean follow-up, 51.7 months). The prevalence of Goutallier grade 2 changes or higher was significantly greater in patients with multitendon repair relative to isolated subscapularis tendon repair. For the overall group of all patients undergoing subscapularis tendon repair, whether in isolation or as part of a multitendon repair, PROMIS-UE scores were significantly lower for patients with infraspinatus muscle grade 2 or higher Goutallier changes relative to grade 0 or 1. After adjustment for age, body mass index, patient sex, and fatty infiltration in other rotator cuff muscles, poor infraspinatus muscle quality remained the only significant predictor for lower PROMIS-UE scores. Conclusion: Patients undergoing arthroscopic subscapularis tendon repair with poor infraspinatus muscle quality had worse patient-reported outcomes. This was true whether subscapularis tendon repair was isolated or was performed in conjunction with supraspinatus and infraspinatus tendon repairs.
Immunoglobulin variable domain high-throughput sequencing reveals specific novel mutational patterns in POEMS syndrome
Authors: Bender Sébastien , Javaugue Vincent , Saintamand Alexis , Ayala Maria Victoria , Alizadeh Mehdi , Filloux Matthieu , Pascal Virginie , Gachard Nathalie , Lavergne David , Auroy Fabienne , Cogné Michel , Bridoux Frank , Sirac Christophe , Jaccard Arnaud 
Published: April 2020

AbstractPolyneuropathy, organomegaly, endocrinopathy, monoclonal gammopathy, and skin changes (POEMS) syndrome is a rare multisystem disease resulting from an underlying plasma cell (PC) dyscrasia. The pathophysiology of the disease remains unclear, but the role of the monoclonal immunoglobulin (Ig) light chain (LC) is strongly suspected because of the highly restrictive usage of 2 λ variable (V) domains (IGLV1-40 and IGLV1-44) and the general improvement of clinical manifestations after PC clone-targeted treatment. However, the diagnostic value of Ig LC sequencing, especially in the case of incomplete forms of the disease, remains to be determined. Using a sensitive high-throughput Ig repertoire sequencing on RNA (rapid amplification of cDNA ends-based repertoire sequencing [RACE-RepSeq]), we detected a λ LC monoclonal expansion in the bone marrow (BM) of 83% of patients with POEMS syndrome, including some in whom BM tests routinely performed to diagnose plasma cell dyscrasia failed to detect λ+ monoclonal PCs. Twenty-four (83%) of the 29 LC clonal sequences found were derived from the IGLV1-40 and IGLV1-44 germline genes, as well as 2 from the closely related IGLV1-36 gene, and all were associated with an IGLJ3*02 junction (J) gene, confirming the high restriction of VJ region usage in POEMS syndrome. RACE-RepSeq VJ full-length sequencing additionally revealed original mutational patterns, the strong specificity of which might crucially help establish or eliminate the diagnosis of POEMS syndrome in uncertain cases. Thus, RACE-RepSeq appears as a sensitive, rapid, and specific tool to detect low-abundance PC clones in BM and assign them to POEMS syndrome, with all the consequences for therapeutic options.
First case of COVID-19 in a patient with multiple myeloma successfully treated with tocilizumab
Authors: Zhang Xuhan , Song Kaidi , Tong Fei , Fei Mingming , Guo Hui , Lu Zhaohui , Wang Jinquan , Zheng Changcheng 
Published: April 2020

Key Points
We report the first case of coronavirus disease 2019 (COVID-19) in a multiple myeloma patient successfully treated with tocilizumab. Although tocilizumab was effective in the treatment of COVID-19 in this case, randomized controlled trials are needed.
The assessment of acute pulmonary embolism severity using CT angiography features
Authors: Shayganfar Azin , Hajiahmadi Somayeh , Astaraki Mohsen , Ebrahimian Shadi 
Published: April 2020

Abstract
Background
This study was conducted to detect the association between radiologic features of CT pulmonary angiography (CTPA) and pulmonary embolism severity index (PESI).

Methods
A total of 150 patients with a definite diagnosis of PE entered the study. The CTPA feature including obstruction index, pulmonary trunk size, presence of backwash contrast, septal morphology, right ventricular (RV) and left ventricular (LV) dimensions, and RV/LV ratio were examined. The severity of the PE was estimated using PESI. The association between CTPA indices and PESI was measured. Statistical analysis was conducted using the SPSS software. P value < 0.05 was considered as statistically significant.

Results
A positive correlation was detected between the obstruction index and PESI (r = 0.45, P < 0.05). Moreover, PESI was significantly higher in patients with a more dilated pulmonary trunk (r = 0.20, P < 0.05). The backwash contrast and abnormal septal morphology were significantly more common among patients with higher PESI (P < 0.05). However, no significant correlation was detected between RV, LV, RV/LV, and PESI. The most predictor of high-risk PE was dilated pulmonary trunk with an odds ratio of 4.4.

Conclusion
Higher Obstruction index, dilated pulmonary trunk, presence of backwash contrast, and an abnormal septal morphology can be associated with a higher PESI.
Modeling of shotgun sequencing of DNA plasmids using experimental and theoretical approaches
Authors: Shityakov Sergey , Bencurova Elena , Förster Carola , Dandekar Thomas 
Published: April 2020

AbstractBackgroundProcessing and analysis of DNA sequences obtained from next-generation sequencing (NGS) face some difficulties in terms of the correct prediction of DNA sequencing outcomes without the implementation of bioinformatics approaches. However, algorithms based on NGS perform inefficiently due to the generation of long DNA fragments, the difficulty of assembling them and the complexity of the used genomes. On the other hand, the Sanger DNA sequencing method is still considered to be the most reliable; it is a reliable choice for virtual modeling to build all possible consensus sequences from smaller DNA fragments.ResultsIn silico and in vitro experiments were conducted: (1) to implement and test our novel sequencing algorithm, using the standard cloning vectors of different length and (2) to validate experimentally virtual shotgun sequencing using the PCR technique with the number of cycles from 1 to 9 for each reaction.ConclusionsWe applied a novel algorithm based on Sanger methodology to correctly predict and emphasize the performance of DNA sequencing techniques as well as in de novo DNA sequencing and its further application in synthetic biology. We demonstrate the statistical significance of our results.Graphical abstract
PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth
Authors: Thejer Bashar M. , Adhikary Partho P. , Kaur Amandeep , Teakel Sarah L. , Van Oosterum Ashleigh , Seth Ishith , Pajic Marina , Hannan Katherine M. , Pavy Megan , Poh Perlita , Jazayeri Jalal A. , Zaw Thiri , Pascovici Dana , Ludescher Marina , Pawlak Michael , Cassano Juan C. , Turnbull Lynne , Jazayeri Mitra , James Alexander C. , Coorey Craig P. , Roberts Tara L. , Kinder Simon J. , Hannan Ross D. , Patrick Ellis , Molloy Mark P. , New Elizabeth J. , Fehm Tanja N. , Neubauer Hans , Goldys Ewa M. , Weston Leslie A. , Cahill Michael A. 
Published: April 2020

Abstract
Background
Progesterone Receptor Membrane Component 1 (PGRMC1) is expressed in many cancer cells, where it is associated with detrimental patient outcomes. It contains phosphorylated tyrosines which evolutionarily preceded deuterostome gastrulation and tissue differentiation mechanisms.

Results
We demonstrate that manipulating PGRMC1 phosphorylation status in MIA PaCa-2 (MP) cells imposes broad pleiotropic effects. Relative to parental cells over-expressing hemagglutinin-tagged wild-type (WT) PGRMC1-HA, cells expressing a PGRMC1-HA-S57A/S181A double mutant (DM) exhibited reduced levels of proteins involved in energy metabolism and mitochondrial function, and altered glucose metabolism suggesting modulation of the Warburg effect. This was associated with increased PI3K/AKT activity, altered cell shape, actin cytoskeleton, motility, and mitochondrial properties. An S57A/Y180F/S181A triple mutant (TM) indicated the involvement of Y180 in PI3K/AKT activation. Mutation of Y180F strongly attenuated subcutaneous xenograft tumor growth in NOD-SCID gamma mice. Elsewhere we demonstrate altered metabolism, mutation incidence, and epigenetic status in these cells.

Conclusions
Altogether, these results indicate that mutational manipulation of PGRMC1 phosphorylation status exerts broad pleiotropic effects relevant to cancer and other cell biology.
Effect of simulated warming on leaf functional traits of urban greening plants
Authors: Zhu Jiyou , Zhu Hua , Cao Yujuan , Li Jinhang , Zhu Qiuyu , Yao Jiangming , Xu Chengyang 
Published: April 2020

AbstractBackgroundResponse and adaptation strategies of plants to the environment have always been the core issues in ecological research. So far, relatively little study exists on its functional traits responses to warming, especially in an urban environment. This information is the key to help understand plant responses and trade-off strategy to urban warming.ResultsWe chose the common greening trees of mature age in Beijing (Fraxinus pennsylvanica, Koelreuteria paniculata, and Sophora japonica) as the research subjects, and used infrared heaters to simulate warming for three gradients of natural temperature (CK), moderate warming (T1) and severe warming (T2). Results showed that:(1) Leaf dry matter content (LDMC), chlorophyll content (CHL), leaf tissue density (LTD), and stomatal density (SD) all increased with temperature warming. Specific leaf area (SLA), stomatal size (SS), and stomatal aperture (SA) decreased with simulated warming. (2) SLA was extremely significantly negatively correlated with CHL, LDMC, LTD and SD (P < 0.01), and was extremely significantly positively correlated with SS (P < 0.01). SA was extremely negatively correlated with SD (P < 0.01), and was extremely significantly positively correlated with SS (P < 0.01). There was a significant positive correlation between LDMC and LTD (P < 0.01). This showed that urban greening trees adapted to the environment by coordinating adjustment among leaf functional traits. (3) Under the T1 treatment, theR2and slope among the leaf traits were higher than CK, and the significance was also enhanced. The correlation between leaf traits was strengthened in this warming environment. Conversely, it will weaken the correlation between leaf traits under the T2 treatment.ConclusionOur study demonstrated that there was a strong trade-off between leaf functional traits in the urban warming environment. Plants in the warming environment have adopted relatively consistent trade-offs and adaptation strategies. Moderate warming was more conducive to strengthening their trade-off potential. It is further verified that the global leaf economics spectrum also exists in urban ecosystems, which is generally tend to a quick-investment return type with the characteristics of thick leaves, strong photosynthetic capacity, low transpiration efficiency and long life in urban environments.
A risk factor-based predictive model for new-onset hypertension during pregnancy in Chinese Han women
Authors: Hou Yamin , Yun Lin , Zhang Lihua , Lin Jingru , Xu Rui 
Published: April 2020

Abstract
Background
Hypertensive disorders of pregnancy (HDP) is one of the leading causes of maternal and neonatal mortality, increasing the long-term incidence of cardiovascular diseases. Preeclampsia and gestational hypertension are the major components of HDP. The aim of our study is to establish a prediction model for pregnant women with new-onset hypertension during pregnancy (increased blood pressure after gestational age > 20 weeks), thus to guide the clinical prediction and treatment of de novo hypertension.

Methods
A total of 117 pregnant women with de novo hypertension who were admitted to our hospital’s obstetrics department were selected as the case group and 199 healthy pregnant women were selected as the control group from January 2017 to June 2018. Maternal clinical parameters such as age, family history and the biomarkers such as homocysteine, cystatin C, uric acid, total bile acid and glomerular filtration rate were collected at a mean gestational age in 16 to 20 weeks. The prediction model was established by logistic regression.

Results
Eleven indicators have statistically significant difference between two groups (P < 0.05). These 11 factors were substituted into the logistic regression equation and 7 independent predictors were obtained. The equation expressed including 7 factors. The calculated area under the curve was 0.884(95% confidence interval: 0.848–0.921), the sensitivity and specificity were 88.0 and 75.0%. A scoring system was established to classify pregnant women with scores ≤15.5 as low-risk pregnancy group and those with scores > 15.5 as high-risk pregnancy group.

Conclusions
Our regression equation provides a feasible and reliable means of predicting de novo hypertension after pregnancy. Risk stratification of new-onset hypertension was performed to early treatment interventions in high-risk populations.
Serum amyloid A is a better predictive biomarker of mucosal healing than C-reactive protein in ulcerative colitis in clinical remission
Authors: Wakai Masaki , Hayashi Ryohei , Tanaka Shinji , Naito Toshikatsu , Kumada Junko , Nomura Motonobu , Takigawa Hidehiko , Oka Shiro , Ueno Yoshitaka , Ito Masanori , Chayama Kazuaki 
Published: April 2020

Abstract
Background
Many studies have revealed that mucosal healing improves the long-term prognosis of ulcerative colitis. Frequent colonoscopy is difficult because of its invasiveness and cost. Therefore, in diagnosing and treating ulcerative colitis, noninvasive, low-cost methods for predicting mucosal healing using useful biomarkers are required in the clinical setting.
This study aimed to evaluate whether serum amyloid A is a better serum biomarker than C-reactive protein in predicting mucosal healing in ulcerative colitis patients in clinical remission.

Methods
Ulcerative colitis patients whose C-reactive protein and serum amyloid A were measured within 1 month before and after colonoscopy were included in this retrospective study, and the relationship between the C-reactive protein and serum amyloid A values and the mucosal condition was analyzed. Mucosal condition was assessed using the Mayo Endoscopic Score, with score 0 or 1 indicating mucosal healing.

Results
A total of 199 colonoscopic examinations were conducted in 108 ulcerative colitis patients who underwent C-reactive protein and serum amyloid A blood tests. In clinical remission patients, serum amyloid A showed a strong correlation with mucosal inflammation compared to C-reactive protein and had excellent sensitivity and specificity rates with significant statistical significance.

Conclusions
Serum amyloid A is a more useful marker compared to C-reactive protein in predicting mucosal inflammation in ulcerative colitis patients in clinical remission.
Incidence, aetiology and related comorbidities of cirrhosis: a Swedish population-based cohort study
Authors: Vaz Juan , Eriksson Berne , Strömberg Ulf , Buchebner David , Midlöv Patrik 
Published: April 2020

Abstract
Background
The incidence of cirrhosis for individuals in Sweden has previously been reported as stable/low among European countries. However, Swedish population-based studies are scarce and none of them included data from the most recent decade (2010–2019). We aimed to describe the incidence and aetiology of cirrhosis in the Halland region from 2011 to 2018, and to describe the severity and prevalence of liver-related complications and other primary comorbidities at the time of cirrhosis diagnosis.

Methods
We conducted a retrospective cohort study of all patients with cirrhosis in Halland, which has a population of 310,000 inhabitants. Medical records and histopathology registries were reviewed.

Results
A total of 598 patients with cirrhosis were identified. The age-standardised incidence was estimated at 23.2 per 100,000 person-years (95% CI 21.3–25.1), 30.5 (95% CI 27.5–33.8) for men and 16.4 (95% CI 14.3–18.7) for women. When stratified by age, the highest incidence rates were registered at age 60–69 years. Men had a higher incidence rate for most age groups when compared to women. The most common aetiology was alcohol (50.5%), followed by cryptogenic cirrhosis (14.5%), hepatitis C (13.4%), and non-alcoholic fatty liver disease (5.7%). Most patients had at least one liver-related complication at diagnosis (68%). The most common comorbidities at diagnosis were arterial hypertension (33%), type 2 diabetes (29%) and obesity (24%).

Conclusions
Based on previous Swedish studies, our results indicate that the incidence of cirrhosis in Sweden might be considerably higher than previously reported. It is uncertain if the incidence of cirrhosis has previously been underestimated or if an actual increment has occurred during the course of the most recent decade. The increased incidence rates of cirrhosis reported in Halland are multifactorial and most likely related to higher incidence rates among the elderly. Pre-obesity and obesity are common in cirrhosis and non-alcoholic fatty liver disease has become an important cause of cirrhosis in Halland.