bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical impact of advance chronic kidney disease in patients with non-HIV pulmonary cryptococcosis
Authors: Tashiro Hiroki , Haraguchi Tetsuro , Takahashi Koichiro , Sadamatsu Hironori , Tajiri Ryo , Takamori Ayako , Kimura Shinya , Aragane Naoko 
Published: March 2020

Abstract
Background: Pulmonary cryptococcosis is an uncommon infectious disease that can develop in both immunocompromised and immunocompetent patients. Severity of chronic kidney disease (CKD) was reported to be one of the risk factors for pulmonary cryptococcosis, but its clinical characteristics have not been fully assessed. The purpose of this study is to clarify clinical characteristics of advance CKD in patients with pulmonary cryptococcosis.Methods: The present study retrospectively investigated 56 patients who had pulmonary cryptococcosis with non-human immunodeficiency virus (HIV) infection and were treated at Saga University Hospital between 2005 and 2018. The clinical characteristics were evaluated and compared between patients with estimated glomerular filtration rate (eGFR) >45 mL/min/1.73m2 (n = 42) (early CKD) and those with eGFR <45 mL/min/1.73m2 (n = 14) (advance CKD).Results: Compared with patients with early CKD, those with advance CKD had significantly higher rate of disseminated cryptococcosis (21.4% vs 2.4%, p = 0.03); lower percentage of patients who recovered after treatment (63.6% vs 92.5%, p = 0.02); and more frequent clinical features of fever (57.1% vs 19.0%, p < 0.01), pleural effusion (21.4% vs 2.4%, p = 0.03), high white blood cell count (8550/ml vs 6150/ml, p = 0.01) and C-reactive protein (2.1 mg/dl vs 0.2 mg/dl, p = 0.02), and low level of serum albumin (3.0 g/dl vs 3.8 g/dl, p <0.01). Multivariate analysis adjusted by immunosuppressive drug use indicated significant differences of fever (odds ratio or β value [95% confidence interval] 6.4 [1.65 – 20.09], p <0.01), high white blood cell count (1293.2 [110.2 – 2476.2], p = 0.03), C-reactive protein (0.89 [0.18 – 1.59], p = 0.01) and low level of serum albumin (- 0.34 [-0.54 – -0.14], p <0.01) in patients with eGFR <45 mL/min/1.73m2.Conclusion: Advance CKD is associated with poor clinical characteristics and outcomes in patients with non-HIV pulmonary cryptococcosis.
ABCD2 and ABCD3-I scores in a TIA population with low stroke risk
Authors: Ildstad Fredrik , Ellekjær Hanne , Wethal Torgeir , Lydersen Stian , Fjærtoft Hild , Indredavik Bent 
Published: March 2020

Abstract
Background Several clinical risk scores have been developed to predict stroke risk after transient ischemic attack (TIA). We aimed to evaluate the ABCD3-I score and compare it with the ABCD2 score in short and long-term stroke risk prediction in our post TIA stroke risk study, MIDNOR TIA. Methods We performed a prospective, multicenter study in Central Norway from October, 2012, to July, 2015, enrolling 577 patients with TIA. In a subset of patients (n=305) we calculated the AUC statistics of the ABCD3-I score and compared this with the ABCD2 score at 1 week, 3 months and 1 year. To assess stroke occurrences, data obtained by telephone follow-up and registry data from the Norwegian Stroke Register was used. Results Three hundred and five patients had complete data for both ABCD3-I and ABCD2 scores. Within 1 week, 3 months and 1 year, 1.0% (n=3), 3.3% (n=10) and 5.2% (n=16) experienced a stroke, respectively. The AUCs for the ABCD3-I score were 0.72 (95% CI, 0.54 to 0.89) at 1 week (compared with ABCD2 score p =0.019), 0.66 (95% CI, 0.53 to 0.80) at 3 months ( p =0.11), and 0.68 (0.95% CI, 0.56 to 0.79) at 1 year ( p =0.39). Conclusions The ABCD3-I score had limited value in short term prediction of subsequent stroke after TIA and did not reliably discriminate between low and high-risk patients in long-term follow-up. The ABCD2 score did not predict subsequent stroke accurately at any time point. Since modern treatment regimens and a decrease in risk factors in the population have contributed to a generally lower stroke risk after TIA during the last years, the benefit of these clinical risk scores and their role in TIA management seems limited.
Difference in osteogenic ability of bone marrow mesenchymal stem cells in patients with hemifacial microsomia on the healthy side versus affected side of the mandible
Authors: huang nan , Li Tianya , Chen Wei , ZHANG Qun , CHAI Gang 
Published: March 2020

Abstract
Background: This study was conducted to examine the differences in the osteogenic ability of bone marrow mesenchymal stem cells (BMSCs) in patients with hemifacial microsomia on the healthy side versus on the affected side of the mandible. Methods: Patients with hemifacial microsomia admitted to Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine received bone marrow blood from the healthy and affected sides of the mandible, and BMSCs were cultured to evaluate their osteogenic induction and differentiation activities. Alizarin red (AR) staining was performed to observe the mineralization nodule formation ability of BMSCs. Alkaline phosphatase (ALP) staining was used to measure alkaline phosphatase activity, and the gene expression of the osteogenic-related indicators osteocalcin, osteopontin, Runx-related transcription factor 2, and ALP was detected by real-time fluorescent quantitative PCR. Differences in osteogenic capacity were also explored. Results: Osteogenesis related staining and Quantitative real-time PCR were performed，the results showed:in AR staining, osteogenesis on the healthy side was significantly better than that on the affected side (P＜0.001) ;in ALP staing, ALP activity of the affected side was significantly lower than that of the healthy side(P＜0.001);in the Quantitative real-time PCR,the osteogenesis-related genes ALP, OCN, OPN, and RUNX2 showed high expression on the healthy side and low expression on the affected side(P＜0.05). Conclusions: The osteogenesis ability of BMSCs differed on the healthy and affected mandibular bone in patients with hemifacial microsomia; the osteogenesis ability on the affected side was significantly lower than that on the healthy side. Keywords: hemifacial microsomia; bone marrow-derived mesenchymal stem cells; osteogenesis
The (cost-)effectiveness and cost-utility of a novel integrative care initiative for patients with chronic musculoskeletal pain: the pragmatic trial protocol of Network Pain Rehabilitation Limburg
Authors: Lamper Cynthia , Huijnen Ivan PJ , Goossens Mariëlle EJB , Winkens Bjorn , Ruwaard Dirk , Verbunt Jeanine AMCF , Kroese Mariëlle E 
Published: March 2020

Abstract
Background: Rehabilitation care for patients with chronic musculoskeletal pain (CMP) is not optimally organized. The Network Pain Rehabilitation Limburg 2.0 (NPRL2.0) provides integrated care with a biopsychosocial approach and strives to improve the Quadruple Aim outcomes: pain-related disability of patients with CMP; experiences of care of patients with CMP; meaning in the work of healthcare professionals; and healthcare costs. Firstly, in this study, the effectiveness (with regard to the functioning and participation of patients) of primary care for patients with CMP will be assessed, comparing care organized following the NPRL2.0 procedure with usual care. Secondly, the cost-effectiveness and cost-utility with regard to health-related quality of life and healthcare costs will be assessed. And thirdly, the effect of duration of participation in a local network in primary care will be studied. Methods: In this pragmatic study, it is expected that two local networks with 105 patients will participate in the prospective cohort study and six local networks with 184 patients in the stepped-wedge based design. Healthcare professionals in the local networks will recruit patients. Inclusion criteria: age ≥ 18 years; having CMP; willing to improve functioning despite pain; and adequate Dutch literacy. Exclusion criteria: pregnancy; and having a treatable medical or psychiatric disease. Patients will complete questionnaires at baseline (T1), 3 months (T2), 6 months (T3), and 9 months (T4). Questionnaires at T1 and T4 will include the Pain Disability Index and Short Form Health Survey. Questionnaires at T1, T2, T3, and T4 will include the EQ-5D-5L, and iMTA Medical Consumption and Productivity Cost Questionnaires. Outcomes will be compared using linear mixed-model analysis and costs will be compared using bootstrapping methods. Discussion: NPRL2.0 is a multidimensional, complex intervention, executed in daily practice, and therefore needing a pragmatic study design. The current study will assess the NPRL2.0 with respect to the Quadruple Aim’s outcomes of effectiveness of healthcare and economic consequences. The other two Quadruple Aim outcomes will be examined alongside this study, providing a broad scope of the performance of NPRL2.0. Trial registration: Netherlands Trial Register: NL7643; Registered 5 April 2019. https://www.trialregister.nl/trial/7643
Epigenetic regulation of osteopontin splicing isoform c defines its role as a microenvironmental factor to promote the survival of colon cancer cells from 5-FU treatment
Authors: Chang Siyuan , Huang Jing , Niu Huan , Wang Jing , Si Yang , Bai Zhigang , Cheng Shan , Ding Wei 
Published: March 2020

Abstract
Background: Drug resistance to 5-fluorouracil (5-FU) and recurrence after chemotherapy in colorectal cancer remain a challenge to be resolved for the improvement of patient outcomes. It is recognized that a variety of secretory proteins released from the tumor cells exposed to chemo-drugsinto the tumor microenvironment (TME) contributedto the cell-to-cell communication, and altered the drug sensitivity.One of these important factors is osteopontin (OPN), which exists in several functional forms from alternative splicing and post-translational processing. In colon cancer cells, increased total OPN expression was observed during the progression of tumors, however, the exact role and regulation of the OPN splicing isoforms was not well understood. Methods: We assayed precisely the abundance of majorOPN splicing isoformsunder5-FU treatmentsincolon cancer cell lineswith different sensitivities to 5-FU, and also evaluated the effects of the condition medium from OPNsplicing isoforms overexpressed cells on cell functions. The methods of nuclear calcium reporter assays and ChIP (chromatin immunoprecipitation) assays were used to investigate the molecular mechanism underlining the production of OPN isoforms.Results: We discovered that OPNc was a most increased splicing isoform to a significant abundance following 5-FU treatment of colon cancer cells. OPNc as a secretory protein in the conditioned medium exerted a more potent effect to promote cell survival in 5-FU than other OPN isoforms. The kinetic response of nuclear calcium signalscouldbe used to indicate an immediate effect of the conditioned medium containing OPNc and other isoforms. MeCP2 was identified to regulate the splicing of opn gene, where the phosphorylation of MeCP2 at S421 site, possibly by CAMKII, was required. Conclusions: The results demonstrated that the production of OPNc was highly controlled under epigenetic regulations, where MeCP2 and the activation of nuclear calcium signaling were involved. It was also suggested that OPNc could transmit the stress signal of cells upon chemotherapy in TME and promoted the survival of adjacent colon cancer cells.
Determinants of health facilities to provide newborn care services in Ethiopia: Finding from service Availability and Readiness Assessment 2018
Authors: Tesema Tefera Taddele 
Published: March 2020

Abstract
Background : Almost 50% of under-five mortality happens during the neonatal period. Sub-Saharan Africa has the highest rates of neonatal mortality. In this study we assessed the availability and readiness of health facility for newborn care service, its variations by health facility type, managing authority, location and regions in Ethiopia. Method: Data were used from the 2018 Ethiopian Service Availability and Readiness Assessment (SARA) from 9 regions and two city administration of the country. The overall newborn care services readiness score was calculated using ten tracer items and its mean availability was created for measuring health facilities overall capacity to provide newborn care services. A multiple linear regression model was used to determine factors associated with the newborn health readiness score. The analysis was done using SPSS version 20 and STATA version 14. Result : A total of 632 health facilities were included in the analysis. The mean availability of newborn care services at a national level was 63%. Only 7% of clinics provided neonatal resuscitation. A higher proportion of public facilities provided all signal functions compared with others facility types. Availability of newborn care services in overall facilities found in Tigray region were higher than facilities found in other regions. The overall newborn readiness score for health centres and clinics were lower than hospitals respectively. The expected value of newborn care readiness to provide the service was lower in health facilities found in Oromiya, Somali and Gambella than health facilities in Tigray. Conclusion : The availability of neonatal resuscitation, corticosteroids, antibiotic for preterm or prolonged premature rupture of membrane and injectable antibiotics for sepsis signal functions and the capacity of health facilities in terms of the mean availability of ten tracer items to provide newborn care services were low in clinics compared with health centres and hospitals. There was regional variation on the availability of the services. So, in the future focus should be given on clinics and the regions whose readiness score was low to ensure equity in distribution and its newborn care service capacity.
An e-registry for household contacts exposed to multidrug resistant TB in Mongolia
Authors: Naker Kush , Gaskell Katherine M , Dorjravdan Munhjargal , Daamba Naranzul , Roberts Chrissy h , Moore David AJ 
Published: March 2020

Abstract
Background: The WHO recommends that individuals exposed to persons with multidrug resistant tuberculosis (MDRTB) should be screened for active TB and followed up for two years to detect and treat secondary cases early. Resource prioritisation means this is rarely undertaken and where it is performed it is usually using a paper-based record, without collation of data. Electronic data collection into a web-based registry offers the opportunity for simplified and systematic TB contact surveillance with automatic synthesis of data at local, regional and national level. This pilot study was designed to explore the feasibility of usage of a novel e-registry tool and explore obstacles and facilitating factors to implementation. Methods: In parallel with their paper records, seven dispensaries in Ulaanbaatar, Mongolia collected standardized data electronically using Open Data Kit (ODK). Patients with MDRTB and their contacts were recruited during a single clinic visit. Staff and patients were interviewed to gain insights into acceptability and to identify areas for improvement. Results: 70 household contacts of 32 MDR-TB index patients were recruited. 7/70 contacts (10%) traced had active TB at the time they were recruited to the e-registry. Paper registry satisfaction was low; 88% of staff preferred the e-registry as it was perceived as faster and more secure. Patients and their contacts were generally supportive of the e-registry; however, a significant minority 10/42 (24%) of index cases who were invited, declined to participate in the e-registry, with data security cited as their top concern. Conclusion: E-registries are a promising tool for MDRTB contact tracing, but their acceptability amongst patients should not be taken for granted.
SET domain-containing protein 4: biological functions and role in genomic methylation profiles of bone marrow mesenchymal stem cells
Authors: Liao Xiao-min , Wu Cai-xia , Shao Zhong-ming , Zhang Shu-ya , Zou Yuan , Yuan Jian-ling , Feng Mu-yin , Ha Yan-ping , Li Ru-jia , Xing Jing-ci , Zheng A-xiu , Shen Zhi-hua , Guo Jun-li , Zheng Shao-Jiang , Jie Wei 
Published: March 2020

Abstract
Background: Epigenetic modification is a crucial mechanism affecting the biological function of stem cells. SETD4 is a histone methyltransferase, and its biological role in bone marrow mesenchymal stem cells (BMSCs) is currently unknown. This work was aimed to reveal the SETD4 biological role as well as its impacts on the genomic methylation profiles in BMSCs. Methods: BMSCs were isolated form SETD4 knockout (KO) and wild type (WT) mice that established by CRISPR/Cas9 technology. The cell proliferation, migration, myogenic differentiation and angiogenesis were tested according to appropriate biology techniques. And the Reduced Representation Bisulfite Sequencing (RRBS) method was adopted to analyze the global genomic methylation profiles of BMSCs, following bioinformatics analysis of GO functions and KEGG signaling of differential methylated CpG sites and differential methylation regions (DMRs). Finally, validation experiments were conducted to examine the expression of histone lysine methyltransferase and some representative genes. Results: SETD4 KO significantly promoted BMSCs proliferation, which was characterized by enhanced cell viability and increased expression of PCNA, Cyclin A2, Cyclin E1, CDK2, CDK6, Bcl2 and decreased the expression of P16, P21 and Caspase3. SETD4 deficiency impaired BMSCs migration and myogenic differentiation potentials, and even the angiogenesis via paracrine of VEGF. Compared with WT control, the overall genomic methylation of BMSCs in the SETD4 KO group only was decreased by 0.47%. However, the changed genomic methylation covers a total of 96,331 differential methylated CpG sites and 8692 DMR, with part of them settled in promoter regions. GO and KEGG analysis revealed that differential CpG islands and DMRs in promotes impacted 270 GO functions and 34 KEGG signaling pathways, with some closely related to stem cell biology. SETD4 KO inhibited sets of monomethylases and dimethylases for histone lysine, along with significant changes in some factors including Nkx2.5, Gata4, Gli2, Grem2, E2f7, Map7, Nr2f2 and Shox2 that associated with stem cell biology. Conclusions: These results are the first to reveal that even though SETD4 changes the genome’s overall methylation to a limited extent in BMSCs, it still affects the numerous cellular functions and signaling pathways, implying SETD4-altered genomic methylation serves a crucial molecular role in BMSCs’ biological functions.
Engaging stakeholders to identify implementation strategies for a faith-based physical activity program
Authors: Haughton Jessica , Takemoto Michelle L. , Schneider Jennifer , Hooker Steven P. , Rabin Borsika , Brownson Ross C. , Arredondo Elva M. 
Published: March 2020

Abstract
Background: Stakeholder engagement is critical to the acceleration of evidence-based interventions into community settings. Harnessing the knowledge and opinions of community stakeholders increases the likelihood of successful implementation, scale up, and sustainment of evidence-based interventions. Faith in Action ( Fe en Acción ) is an evidence-based promotora -led physical activity program designed to increase moderate-to-vigorous physical activity among churchgoing Latina women.Methods: We conducted in-depth interviews using a semi-structured interview guide based on the Consolidated Framework for Implementation Research (CFIR) at various Catholic and Protestant churches with large Latino membership in San Diego County, California to explore barriers and facilitators to implementation of a Faith in Action and to identify promising implementation strategies for program scale-up and dissemination. We interviewed 22 pastors and church staff and analyzed transcripts using an iterative-deductive team approach.Results: Stakeholders described barriers and facilitators to implementation within three domains of CFIR: characteristics of individuals (lack of self-efficacy for and knowledge of PA; influence on churchgoers’ behaviors), inner setting (church culture and norms, alignment with mission and values, competing priorities, lack of resources), and outer setting (need for buy-in from senior leadership). From the interviews, we identified four promising implementation strategies for the scale-up of faith-based health promotion programs: 1) health behavior change training for pastors and staff; 2) tailored messaging; 3) developing community collaborations; and 4) gaining denominational support.Conclusions: While churches can serve as valuable partners in health promotion, specific barriers and facilitators to implementation must be recognized and understood. Addressing these barriers through targeted implementation strategies at the adopter and organizational level can facilitate improved program implementation and lead the way for scale-up and dissemination.
Oxycodone reduced postoperative catheter-related bladder discomfort undergoing transurethral resection prostate. A prospective, double -blind randomized study
Authors: li jian 
Published: March 2020

Abstract
Background: Catheter-related bladder discomfort (CRBD) is a common and distressing complication that often occurs caused by urinary bladder catheterization and urethral mucosa injury postoperatively. Oxycodone is a semi-synthetic opioids prepared from opium alkaloid the baine plant derivative. Its μ and κ dual-receptor agonism has a unique effect in the treatment of visceral pain. The aim of this study to observe the efficacy of oxycodone for the treatment of CRBD undergo trans-urethral resection prostate (TURP). Methods: Patients with ASA I-III received trans-urethral resection prostate under general anesthesia were enrolled. Patients who complained CRBD were randomized allocated to the control group (n=42) received placebo and the observed group (n=41) received 0.03mg/kg of oxycodone. The severity of CRBD assessed by NRS were assessed at 0, 5min, 1/2h, and 2h after administration of the study agents. VAS scores were used to assess pain intensity during the same period. Postoperative PCA analgesic sufentanil dose during of PACU times and the incidences of agitation, nausea, vomiting, dizziness, over sedation were recorded in these patients. Results: Compared with the control group, the incidence of CRBD was significantly lower in the oxycodone group at 5min and 1/2h. Compared with the controlled group, VAS scores and incidences of agitation were lower in oxycodone group and significantly decreased sufentanil dosage within 6h (P<0.01). There were no significant differences in the incidence of postoperative adverse effects and during of PACU between two groups(P＜0.05). Conclusion: Oxycodone 0.03mg/kg effectively reduced patients with CRBD after TURP without incurring serious adverse effects.Trial registration: Chinese Clinical Trial Registry, ChiCTR-IPR-16008814.