bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pendampingan pembuatan media pembelajaran Powtoon bagi guru Sekolah Dasar Gugus 1 Kota Bogor
Authors: Sukmanasa Elly , Novita Lina , Maesya Aries 
Published: July 2020

[Bahasa]: Pembelajaran abad 21 menuntut guru untuk memahami, mengenali, dan mampu menggunakan teknologi dengan baik. Sejalan dengan itu proses pembelajaran Kurikulum 2013 juga memerlukan kemampuan guru dalam bidang teknologi terutama dalam pembuatan media pembelajaran yang menarik. Salah satu permasalahan yang terjadi di sekolah Gugus 1 Kota Bogor adalah keterbatasan kemampuan guru dalam menggunakan media pembelajaran berbasis teknologi. Salah satu solusi dari permasalahan ini adalah pelaksanaan pendampingan pembuatan media Powtoon kepada guru-guru Sekolah Dasar (SD) melalui pemberian materi secara teoritis, praktek pembuatan Powtoon, sampai kepada evaluasinya. Tujuan program pengadian kepada masyarakat (PKM) ini adalah untuk melakukan pendampingan pembuatan media pembelajaran Powtoon pada pembelajaran kurikulum 2013 tematik kelas V SD Gugus 1 Kota Bogor. Metode yang digunakan adalah ceramah dan drill practice. Tahapan kegiatan diawali dengan penyampaian materi pentingnya media, pengenalan media Powtoon, dan dilanjutkan dengan drill practice yang mendorong peserta melakukan praktik pembuatan media Powtoon tersebut. Hasil pelatihan PKM ini menunjukkan sebanyak 91,2% peserta belum pernah mendengar tentang media Powtoon dan sebanyak 97,2% peserta belum pernah membuatnya dalam pembelajaran. Setelah dilaksanakannya PKM ini sebanyak 82,4% peserta mampu membuat media Powtoon dengan baik. Kegiatan PKM ini efektif membantu guru untuk merancang pembelajaran berbasis teknologi khususnya media pembelajaran Powtoon.
Kata Kunci: pembelajaran abad 21, pendampingan, media Powtoon
[English]: The 21st-century learning requires teachers to understand, recognize, and be able to use technology properly. In this case, the 2013 Curriculum (K13) learning process also requires teachers’ technological ability to make attractive learning media. One of the problems found in the Cluster 1 Schools in Bogor is the limited ability of teachers to use technology-based learning media. The alternative to this problem is the training in making Powtoon to elementary school (SD) teachers through theoretical material delivery, practice, and evaluation. The purpose of the community service program is to provide assistance in making Powtoon for thematic learning in grade V Cluster 1 Bogor City. The method used was lecture and drill practice. It started with the presentation of the importance of media in learning, the introduction of Powtoon, and following by drill practice that encourages participants to practice the making of Powtoon. The results showed that 91.2% of participants had never heard of Powtoon media and 97.2% of participants had never made it in learning. After the implementation of PKM, 82.4% of the participants were able to make Powtoon. This program is effective in helping teachers to design technology-based learning, especially Powtoon.
Keywords: 21st-century learning, accompaniment, Powtoon media
Peningkatan kesadaran pentingnya pendidikan keluarga melalui pelatihan parenting di Desa Kauman Kecamatan Sedayu Kabupaten Gresik
Authors: Suhartono Suhartono , Atnuri Atnuri , Lestari Bernadetta Budi 
Published: July 2020

[Bahasa]: Keluarga merupakan lingkungan awal bagi anak yang memiliki peran strategis dalam mendukung penyelenggaraan pendidikan. Oleh karenanya, pemahaman tentang pentingnya parenting sangat dibutuhkan untuk meningkatkan kualitas pendidikan keluarga. Tujuan pelaksanaan kegiatan pengabdian kepada masyarakat (PKM) ini adalah untuk memberikan pemahaman tentang pentingnya parenting dalam keluarga yang merupakan tanggungjawab bersama dari orang tua, masyarakat, sekolah, dan pemerintah. Kegiatan PKM ini dilaksanakan di Desa Kauman, Kecamatan Sedayu, Kabupaten Gresik. Metode yang digunakan dalam kegiatan ini adalah pelatihan dengan ceramah, diskusi, dan games. Materi yang diberikan dalam pelatihan ini meliputi pengertian, aturan, penerapan parenting yang tepat, pihak-pihak yang harus berperan dalam terwujudnya parenting, dan bahaya yang ditimbulkan jika parenting dilakukan secara tidak tepat. Hasil kegiatan ini menunjukkan pemahaman peserta terhadap parenting sebelum dilaksanakan kegiatan adalah sebesar 16%. Setelah mengikuti pelatihan dalam PKM ini, pemahaman peserta terhadap parenting adalah sebesar 83%. Hasil ini menunjukkan kegiatan PKM ini dapat meningkatkan pemahaman masyarakat tentang parenting yang bekontribusi pada kesadaran masyarakat terhadap pentingnya pendidikan keluarga.
Kata Kunci: parenting, orang tua, masyarakat, pemerintah
[English]: Family is the initial environment for children who have a strategic role in supporting the delivery of education. Therefore, an understanding of the importance of parenting is needed to improve the quality of family education. This community service program aims to provide the parents an understanding of the importance of parenting in the family as a shared responsibility of parents, community, schools, and government. This progam was conducted in Kauman Village, Sedayu District, Gresik Regency. The method used was training with lectures, discussions, and games. The material provided in this training included the understanding, rules, proper application of parenting, parties who must play a role in the realization of parenting, and the dangers posed if parenting is done incorrectly. The results showed that participants' understanding of parenting before the training was 16%. After attending the training, participants' understanding of parenting was 83%. This shows that this program increases parents’ understanding of parenting that contributes to community awareness of the importance of family education.
Keywords: parenting, parents, society, government
Differentiation of Transbaikal Territory by Tick-Borne Viral Encephalitis Incidence
Authors: Turanov A. O. , Nikitin A. Ya. , Andaev E. I. , Balakhonov S. V. , Shashina N. I. 
Published: July 2020

Objective of the study was to differentiate the Administrative Districts of theTransbaikalTerritory at the time of Tick-borne viral encephalitis (TBVE) incidence recession by epidemiological risk groups and to characterize them by volume of specific and nonspecific preventive measures.Materials and methods. Retrospective analysis of TBVE epidemiological situation is based on the statistical reporting data “Information on infectious and parasitic diseases” in 2009–2019 and other materials of the Rospotrebnadzor Administration in the Transbaikal Territory. The clustering of areas with various levels of epidemiological risk was conducted by calculation of 95 % confidential interval for long-term annual average of TBVE cases in municipal units of the Territory over a decade and assessment of appurtenance of the deviating values to the aggregate under study.Results and discussion. Twenty four out of 32 districts of the Transbaikal Territory are endemic for TBVE. These areas are divided into five groups: with very high epidemiological risk (2 districts), high (5), medium (8), and low (8) risk respectively, as well as the administrative center of the constituent entity which by the whole complex of indicators (disease manifestation, population density, factors of targeted TBVE decrease, social-and-living and economical conditions) cannot be considered together with the rest of municipalities. Each group of the districts was characterized by the number of cases and TBVE incidence rates, medical aid seeking by persons who suffered from tick bites, vaccination volumes, seroprevention, areas of acaricide treatments. Recommendations are presented for the essential complex and scope of measures to prevent TBVE in the groups of administrative districts that differ by the level of epidemiological risk.
Recombinant Retroviral Particles: Technology of Poduction and Application as Positive Controls for PCR Diagnostics of Dangerous Viral Infections
Authors: Fomina E. G. , Grigorieva E. E. , Vladyko A. S. 
Published: July 2020

Objective. Construction of positive control samples based on recombinant retroviral particles and their application in RT-PCR diagnostic assays for RNA detection of agents of dangerous and particularly dangerous viral infections.Materials and methods. Molecular biological, genetic engineering, and immunological methods were used: polymerase chain reaction, restriction, ligation, cloning, transformation, transfection, flow cytometry.Results and discussion. Technology of positive control samples producing based on recombinant virions has been developed and tested. It includes construction of retroviral vector with cloned diagnostic sequence of the viral genome; obtaining a packaging cell line producing chimeric retroviral particles; determination of recombinant virions titer by flow cytometry and polymerase chain reaction; application of the obtained preparation as a control sample for PCR diagnostics of infectious agents. Positive controls based on retroviral vectors as carriers of genomic RNA fragments of pathogenic viruses were used in the development of PCR diagnostic kits for dangerous and particularly dangerous viral infections. Their application increased the kits quality and made it possible to exclude the work with concentrated hazardous infectious agents (Lassa virus, tick-borne encephalitis virus, lymphocytic choriomeningitis virus, Puumala virus).
Search for Genetic Markers of Tick-Borne Encephalitis Virus for Its Specific Indication, Genomic Analysis
Authors: Khammadov N. I. 
Published: July 2020

Objective of the study is to analyze the genetic markers of tick-borne encephalitis virus, which can be used to specifically indicate the maximum number of virus strains and isolates.Materials and methods. Plasmid DNA and nucleic acids of the tick, the genus Ixodes and Dermacentor, were used as amplified material. Polymerase chain reaction (PCR) was conducted on C1000 amplifier with a CFX96 optical unit (BioRad). The species (strain) variety of detected organisms, using the analyzed genetic markers, was determined in the nBLAST software utility. The design of the nucleotide sequences of primers and probes was performed using “Vector NTI 9.1.0” (Invitrogen Corporation). Nucleic acids were isolated by magnetic sorption with a reagent panel MAGNO-sorb. Primers and probes were synthesized at “Evrogen” company, Moscow, Russia. The reagents for PCR were manufactured by “Syntol” company, Moscow, Russia.Results and discussion. When indicating the genome of the tick-borne encephalitis virus, the main criterion for choosing a marker sequence is the specific detection of nucleic acids of only the desired microorganism. The genomewide nucleotide sequences of various strains and isolates of tick-borne encephalitis virus were analyzed to search for a specific marker nucleotide sequence. Mutual comparison of all the above mentioned genomes made it possible to determine 7 conventionally conservative loci characterized by minimal nucleotide variability. The further work was based on the results of alignment of the nucleotide sequences of tick-borne encephalitis virus isolates, including the nucleotide sequences of heterogeneous microorganisms; primers and probes were designed to amplify each of the marker loci; the most analytically significant oligonucleotides were developed based on loci 4 and 7. Amplification with oligonucleotide primers to indicate tick-borne encephalitis virus was effective, both in a separate PCR with a positive control, and in combination with tick DNA.
Results of Epizootiological Survey along the Border Areas of Kharkhira-Turgensky Natural Plague Focus between Russia and Mongolia in 2019
Authors: Kholin A. V. , Sharakshanov M. B. , Verzhutsky D. V. , Korzun V. M. , Orgilbayar L. , Gankhuyag Ts. , Gandbold D. , Tsogbadrakh N. , Tserennorov D. , Tserendulam B. , Erdenedelger G. , Pagmadulam N. , Badamtsetseg M. , Buzhinlkham L. , Erdenetsetseg Ya. , Amarsanaa G. , Altangerel Ya. , Balakhonov S. V. 
Published: July 2020

Objective of the study was to assess the current epizootic settings along the border part of KharkhiraTurgensky natural plague focus betweenRussia andMongolia.Materials and methods. Epizootiological survey covered 2715.5 km2. 213 samples of field material were tested (90 specimens of mammals, 102 specimens of ectoparasites, 17 samples of leftover food of predatory birds and dry skeletal remnants of marmots, 4 regurgitates of birds of prey). Laboratory works were carried out in “Microbiological laboratory for express diagnostics” mounted on the platform of the minibus “GAZelle”. Tests of field material were performed using immune-chromatographic (IC) assay and polymerase chain reaction (PCR). PCR and IC positive samples were further investigated applying bacteriological method. Epizootiological surveyed deployed GIS instruments. All the results obtained were plotted on the electronic maps using QGIS 2.18.26 software.Results and discussion. Capsular antigen (F1) of Yersinia pestis was detected in three (1.4 %) studied samples (n=213), DNA of plague microbe – in eight samples (3.7 %). Bacteriological investigation of positive samples revealed one sample (leftover food of predatory birds – Mongolian marmot) from which plague agent culture was isolated. The culture belonged to Y. pestis of the main subspecies. Geographical positioning of the epizootiological survey sites was conducted, as well as positive findings of immunological and molecular-genetic assays. Results of epizootiological survey are indicative of active phase of plague agent circulation (main subspecies) in Kharkhira-Turgensky natural focus in Mongolia.
Reporting of <i>Aedes (Stegomyia) albopictus</i> (Skuse, 1895) in the Territory of Crimea
Authors: Kovalenko I. S. , Yakunin S. N. , Abibulaev D. E. , Vladychak V. V. , Boroday N. V. , Smelyansky V. P. , Fomina V. K. , Zinich L. S. , Tikhonov S. N. 
Published: July 2020

Abstract. Detection of epidemiologically significant mosquitoes Ae. аlbopictus in the south of Russia (the Greater Sochi region, the Black Sea coast of Caucasus) suggested the possibility of their introduction into Crimea.Objective was to determine the possibility of existence of epidemically significant vector – mosquito Ae. albopictus inCrimea.Materials and methods. Within the frames of entomological monitoring, mosquitoes were collected during May-October, 2018 and May-September, 2019 in different regions of Crimea: Bakhchisaray, Dzhankoy, Razdolnensky, Saksky, Black Sea regions, urban areas ofSimferopol,Sevastopol and Feodosiya. Fully-grown mosquitoes were caught by automatic trap Mosquito Magnet Executive (USA) with octenol-based bait. Actively attacking females were collected with an aspirator by Gutsevich method. The survey was conducted in the evening and night hours. A total of 26 visits were made, 77 hours were spent, and 8463 mosquitoes (adult and larvae) were captured.Results and discussion. During entomological monitoring of the territory of Crimean Peninsula for the first time bloodsucking mosquitoes Aedes (Stegomyia) albopictus (Skuse, 1895) were found. Given the favorable climatic conditions in Crimea, the trend towards global warming, the adaptation of mosquitoes to the periods of cold, biology of Aedes albopictus, the distribution of this species in the European part of southern Russia, the formation and dissemination of sustainably replicating vector population on the Crimea Peninsula is possible. To confirm the circulation of independent Ae. albopictus population inCrimea, not a random import of adult specimens who die in winter, further entomological surveys are required on a regular basis.
Experimental Evaluation of Application of the Vaccine Strain <i>Yersinia pestis</i> EV NIIEG in Combination with Immune-Modulators
Authors: Goncharova A. Yu. , Bugorkova S. A. , Kudryavtseva O. M. , Kozhevnikov V. A. , Kravtsov A. L. , Kashtanova T. N. , Shchukovskaya T. N. 
Published: July 2020

Abstract. Objective of the work was to conduct a comparative assessment of the immune-modulating effect of the combined use of Yersinia pestis EV NIIEG vaccine strain with Polyoxidonium and Ingaron preparations on a BALB/c mouse model.Materials and methods. Mice of the BALB/c line were immunized subcutaneously with Yersinia pestis EV NIIEG culture at a dose of 2.5 104 m.c. (1st group), in combination with Ingaron at a dose of 150 IU (2nd group) or with Polyoxidonium at a dose of 4 мg (3rd group), the 4th group is intact mice. On days 3, 7, 21 and 90 after immunization, the subpopulation composition of lymphocytes, the production of mediators of the cellular response (INF-ɣ and IL-10), the titers of specific antibodies to the capsular antigen of plague microbe (F1), the nuclear apparatus of lymphocytes, and the nature of histological changes in the organs of mice were determined. Characterization of immunogenic (protective) activity of the combined use of Y. pestis EV NIIEG with immune-modulators against Y. pestis 231 in experiments on BALB/c mice was performed on the 21st day after immunization through determining the number of dead animals and their average life expectancy.Results and discussion. The combined administration of Y. pestis EV NIIEG vaccine strain with Polyoxidonium or Ingaron to experimental animals allowed us to establish differences in the response of the immune system of biomodels, due to the mechanism of action of a specific immune-modulator. It has been established that both Polyoxidonium and Ingaron combined with Y. pestis EV NIIEG enhance the response of immune-competent cells in experimental animals, contribute to the activation of the humoral response and the production of mediators of the cellular response, do not have a damaging effect on the tissue of the macroorganism. At the same time, the efficacy of using combined vaccination of Y. pestis EV NIIEG with immune-modulators in the inoculation test is confirmed for Polyoxidonium only.
Phylogeny and Historical-Geographical Analysis of <i>Yersinia pestis</i> Strains from Vietnam
Authors: Nikiforov K. A. , Kukleva L. M. , Al’khova Zh. V. , Oglodin E. G. , Makashova M. A. , Naryshkina E. A. , Guseva N. P. , Eroshenko G. A. , Dang Hong Chien , Lyong Mo Thi , Vo Viet Kyong , Kutyrev V. V. 
Published: July 2020

Objective of the work was to identify molecular-genetic peculiarities, to conduct whole genome sequencing and phylogenetic analysis of Yersinia pestis strains isolated inVietnam between 1962 and 1989.Materials and methods. We have studied the properties of 50 Y. pestis strains, carried out whole genome sequencing of 18 and fragment sequencing of 32 strains from Vietnam. Phylogenetic analysis was performed on the basis of whole genome SNPanalysis by 1391 identified SNPs. Whole genome SNP-analysis and search for marker SNPs were conducted applying Wombac 2.0 software package. Phylogenetic diagram construction was done using Maximum Likelihood algorithm.Results and discussion. Several phylogenetic branches and Y. pestis populations coinciding with geographical and historical dissemination of the strains have been distinguished. The major part of the strains from Vietnam falls under the branch designated by us as 1.ORI2v. Two strains form a separate branch together with the strain from India belonging to 1.ORI2 line, one more strain, 55-801 Saigon, is set among the strains of 1.ORI1 line. Based on the data obtained and evidence from the literature sources it can be assumed that introduction of plague into Vietnam occurred through several waves: Nha Trang city in 1898, by sea; north provinces of the country – 1908. The second wave of Y. pestis dissemination across the territory of Vietnam began in 1960s with the emergence of the strains from the natural plague focus in Yunnan province, China.
Chemical entrapment and killing of insects by bacteria
Authors: Ho Louis , Daniel-Ivad Martin , Jeedigunta Swathi , Li Jing , Iliadi Konstantin , Boulianne Gabrielle , Hurd Thomas , Smibert Craig , Nodwell Justin 
Published: July 2020

Abstract
Actinobacteria such as the filamentous streptomycetes are widely known for their ability to produce specialized metabolites that include antibacterial and antifungal compounds. In addition, a growing body of work demonstrates that many insects harbour actinobacteria on their bodies and in their nests. The result of these mutualistic relationships is the protection of their offspring or food sources by virtue of the bacterially encoded specialized metabolites. However, some actinobacteria produce molecules that are toxic to insects and the relevance of this toxicity in nature is unknown. We have explored interactions between streptomycetes and the fruit fly Drosophila. We find that many streptomycetes produce specialized metabolites that have potent larvicidal effects against the fly. Larvae that ingest spores of the species that produce these toxic molecules die as a result. Strikingly, the mechanism of toxicity is specific to the bacterium’s chemical arsenal: cosmomycin D producing cells induce a relatively slow-acting cell death-like response in the larval digestive tract and avermectin producing cells induce rapid onset, whole-body paralysis. We further show that fruit flies are attracted to the volatile terpene 2-methylisoborneol that is produced by most streptomycetes. This interaction can influence their food choice and egg-laying destination such that they preferentially deposit their eggs on contaminated food sources. As a result, the larvae that hatch in these toxic environments are subsequently killed. This phenomena of terpene-mediated attraction and specialized metabolite toxicity must pose a significant risk to insects in nature.
Segmentation and Evaluation of COVID-19 Lesion from CT scan Slices - A Study with Kapur/Otsu Function and Cuckoo Search Algorithm
Authors: Satapathy Suresh Chandra , Hemanth D. Jude , Kadry Seifedine , Manogaran Gunasekaran , Hannon Naeem M S , Rajinikanth V. 
Published: July 2020

Abstract
Infection/disease in lung is one of the acute illnesses in humans. Pneumonia is one of the major lung diseases and each year; the death rate due to the untreated pneumonia is on rise globally. From December 2019; the pneumonia caused by the Coronavirus Disease (COVID-19) has emerged as a global threat due to its rapidity. The clinical level assessment of the COVID-19 is normally performed with the Computed-Tomography scan Slice (CTS) or the Chest X-ray. This research aims to propose an image processing system to examine the COVID-19 infection in CTS. This work implements Cuckoo-Search-Algorithm (CSA) monitored Kapur/Otsu image thresholding and a chosen image segmentation procedure to extract the pneumonia infection. After extracting the COVID-19 infection from the CTS, a relative assessment is then executed with the Ground-Truth-Image (GTI) offered by a radiologist and the essential performance measures are then computed to confirm the superiority of the proposed technique. This work also presents a comparative assessment among the segmentation procedures, such as Level-Set (LS) and Chan-Vese (CV) methods. The experimental outcome authenticates that, the results by Kapur and Otsu threshold are approximately similar when the LS is implemented and the CV with the Otsu presents better values of Jaccard, Dice and Accuracy compared to other methods presented in this research.