bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regional scale diversity and distribution of soil inhabiting Tetracladium
Authors: Lazar Anna , Griffiths Robert I. , Goodall Tim , Norton Lisa R. , Mushinski Ryan M. , Bending Gary D. 
Published: December 2024

AbstractThe genus Tetracladium has historically been regarded as an aquatic hyphomycete. However, sequencing of terrestrial ecosystems has shown that Tetracladium species might also be terrestrial soil and plant-inhabiting fungi. The diversity of Tetracladium species, their distribution across ecosystems, and the factors that shape community composition remain largely unknown. Using internal transcribed spacer (ITS) amplicon sequencing, we investigated the spatial distribution of Tetracladium in 970 soil samples representing the major ecosystems found across the British landscape. Species of the genus were found in 57% of the samples and across all vegetation types. The Tetracladium sequences we recovered included species common in aquatic ecosystems. However, we found five additional clades that clustered with environmental sequences previously found in terrestrial environments. The community composition of the Tetracladium OTUs was mainly related to vegetation type and soil pH. Strikingly, both taxon richness and overall abundance were highest in arable soils and showed positive relationships with soil pH. T. maxilliforme and a taxon of environmental sequences, Tetracladium group 1, was the biggest group, had the most relative abundance across ecosystems and was found in all vegetation types. Overall, this study provides insights into the community composition patterns of Tetracladium in terrestrial ecosystems and highlights the importance of vegetation characteristics in shaping Tetracladium communities.
Successful analgesic treatment with continuous sacral epidural ethanol injection therapy for anal pain caused by multiple metastases of malignant pheochromocytoma
Authors: Sato Takehito , Inoue Shigeru , Asano Ichiko , Ando Takahiro , Shibata Yasuyuki 
Published: December 2024

Abstract
Background
Anal and perineum pain caused by malignant tumor invasion is often difficult to control with opioids. Continuous sacral epidural ethanol injection therapy is less likely to cause bladder and rectal disturbances, making it a suitable treatment option for patients with preserved voiding function.

Case presentation
A 45-year-old woman with multiple metastases of malignant pheochromocytoma suffered severe anal pain that worsened, especially when sitting, and was unresponsive to opioid rescue therapy.

With her NRS score of 9, a sacral epidural catheter was placed, and a continuous infusion of 2% lidocaine was administered overnight. This is followed by a 1.5mL bolus of ethanol and continuous ethanol administration at 2 mL/h. After administration, her anal pain decreased to approximately NRS 0–1, and she was subsequently discharged.

Conclusion
We report successful pain control using continuous sacral epidural ethanol injection therapy in a patient with anal pain due to malignant pheochromocytoma metastasis.
Exploring the association between gender equality in the family and fertility intentions: an explanation of the findings in low-fertility countries
Authors: Leocádio Victor , Verona Ana Paula , Wajnman Simone 
Published: December 2024

AbstractPrevious research has demonstrated a positive association between gender equality in the family and fertility measures. However, this association may vary when different features of gender equality in the family, such as the division of housework and childcare, are analyzed. Furthermore, it has been argued that individuals may have different perceptions regarding housework and childcare, which can further explain how the division of these activities is associated with fertility outcomes. We performed a microlevel investigation of the association between the division of housework and childcare and fertility intentions with the aim of identifying patterns (or their absence) in the association. We hypothesized that a more equitable division of tasks is associated with increased fertility intentions. We used data from the first round of the Generations and Gender Survey (GGS) in ten countries: Germany, Belgium, Austria, France, Lithuania, Poland, Romania, Russia, Bulgaria, and Georgia. Our samples included individuals who were in heterosexual relationships and had one child. We employed binary logistic regression for analysis. Overall, our findings suggested that a more equitable division of housework was associated with an increased likelihood of intending to have a second child. Conversely, a less equitable division of childcare was associated with a decreased likelihood of intending to have a second child. Owing to the discussion of the diverse perceptions that individuals may hold regarding housework and childcare, this study contributes to the literature by proposing a theoretical explanation for the patterns observed in our results. The results align with those of previous studies indicating that childcare is generally viewed as enjoyable and rewarding, whereas housework tends to be negatively evaluated and linked to perceptions of unfairness and inequality. Consequently, a more equitable division of housework may reduce work‒family conflict and potentially be associated with increased fertility intentions. In contrast, if childcare is perceived as fulfilling, engaging in more childcare activities may lead to increased fairness rather than unfairness, leading to the association between less equal division of childcare and increased fertility intentions.
A comparison of routine [68Ga]Ga-PSMA-11 preparation using Locametz and Illuccix kits
Authors: Wang Ivan E. , Morrissette Luke J. , Wong Ka Kit , Brooks Allen F. , Dakanali Marianna , Scott Peter J. H. 
Published: December 2024

Abstract
Background
Approval of Locametz and Illuccix kits for the manufacture of [68Ga]Ga-PSMA-11 (gallium Ga68 gozetotide), a PET imaging agent for prostate cancer, as well as the corresponding therapeutic ([177Lu]Lu-PSMA-617 Pluvicto), has led to a rapid increase in demand for [68Ga]Ga-PSMA-11 PET imaging. Radiopharmaceutical manufacturers, using 68Ge/68Ga generators, may decide to adopt Locametz and/or Illuccix kits, which requires a comparison to select the most suitable kit for day-to-day use. The objective of this article is to compare both kits and provide guidance for selecting one for routine use, as well as evaluate labeling consistency of both kits during routine production. Additionally, we report our experience during 1.5 years of daily [68Ga]Ga-PSMA-11 production at our facility using both kits.

Results
Locametz (n = 181) and Illuccix (n = 256) kits were prepared using non-silicone coated and silicone-coated needles with 68Ga activities ranging from 0.53 to 3.16 GBq, with a failure rate of 1 in 128 runs for both kits. With Locametz, a 3.7 GBq generator and 10-min incubation at room temperature gave doses that passed quality control (QC) testing. Use of non-silicone coated needles in the process led to solution discoloration, and QC failure. Additionally, lack of vial inversion led to inconsistent labeling, which improved with subsequent vial agitation. For Illuccix, addition of the acetate buffer to the precursor vial prior to adding the [68Ga]GaCl3 simplifies the workflow. The maximum tolerated activity was 1.85 GBq. Lack of vial inversion led to failures, which were rectified by agitating the vial to properly incorporate the acetate solution with the generator eluate.

Conclusions
Both kits benefited from using a syringe pump to elute the 68Ge/68Ga generator, vial agitation, and longer length/smaller bore silicone coated needles. Both kits have similar workflows, comparable QC outcomes, and result in equivalent clinical images. Thus, the decision between kits will ultimately be determined by production preferences. Since radiopharmacies have an established “kit-based” workflow, Locametz kits with higher allowed activities and longer shelf-life may offer benefits. Conversely, more traditional PET manufacturing facilities might benefit from using Illuccix kits due to compatibility with cyclotron-produced [68Ga]GaCl3 allowing for kit batching. Ultimately, the commercial availability of 2 approved kits for production of [68Ga]Ga-PSMA-11 PET has facilitated ready access to this important new imaging agent.
A systematic review of qualitative studies examining barriers and facilitators to orthopaedic surgeon engagement with patient-reported outcome measures data
Authors: Heath Emma L. , Harris Ian A. , Romero Lorena , Ackerman Ilana N. 
Published: December 2024

Abstract
Background
Orthopaedic surgeon engagement with patient-reported outcome measures (PROMs) data has not been comprehensively evaluated, despite increasing uptake of orthopaedic PROMs programmes globally. The aim of this review was to systematically identify, appraise and synthesise qualitative evidence on barriers and facilitators to orthopaedic surgeons’ engagement with PROMs data and their use of these data to support clinical practice.

Methods
Six databases (MEDLINE, EMBASE, COCHRANE CENTRAL, PSYCINFO, CINAHL and EMCARE) were searched from January 2000—March 2024 to identify potentially eligible qualitative studies. Established systematic review methods were used for screening and data extraction, applying PRISMA guidelines. Quality assessment was undertaken using the Joanna Briggs Institute tool for qualitative research.

Results
Eight studies were eligible for inclusion; of these, five studies were qualitative and three studies were mixed-method designs incorporating a qualitative component. Three studies were specific to orthopaedic surgeons and the remaining five studies comprised of mixed samples of health professionals including orthopaedic surgeons. Only one study was classified as being of high methodological quality. Key barrier themes for orthopaedic surgeons were logistical issues, difficulty interpreting and understanding PROMs, and scepticism of the value of PROMs in clinical care. Key enabler themes included improvements to PROMs infrastructure, surgeon education around the potential value, uses and interpretation of PROMs data, aggregate reporting of PROMs data and early involvement of surgeons in the planning and development of PROM systems.

Conclusion
While these studies highlight some practical considerations and opportunities that can be addressed through clinician education, there is little high-quality evidence on factors that influence orthopaedic surgeon engagement with PROMs data. Robust qualitative research is needed to better inform tailored support and assist surgeons in integrating PROMs data within orthopaedic care.
Assessing usability of electronic patient-reported outcome measures in older people with and without a rare dermatologic disorder
Authors: Ho Calvin N. , Kündig Anna , Bahadori Lila , Roat Katy , Bruce Rachel , Goswami Caroline P. , Kelly Kimberly , Moll Thomas 
Published: December 2024

Abstract
Background
Robust and well-defined data collection is important when using electronic patient-reported outcome measures (ePROMs) in clinical studies. Questions have been raised as to whether older age may be a barrier to data collection due to patients’ unfamiliarity with electronic devices. Older adults may also have underlying health conditions that affect their ability to fill out patient-reported outcome measures (PROMs) on electronic devices. The aim of this observational, qualitative research study was to evaluate the usability of electronic PROMs (ePROMs) on a tablet and smartphone in older participants with and without bullous pemphigoid (BP).

Methods
Older people with and without BP were recruited in the US and France. They participated in 60-min in-person interviews, with moderators observing their completion of various tasks, including ePROMs, using a tablet and smartphone. Participants were scored on ease of task completion using a scale from 1 to 5.

Results
A total of 12 participants were recruited (≥65 years old; six each with and without BP [all participants without BP were ≥75 years old]). Most participants (83%) could easily and confidently perform most assigned tasks on both the tablet and smartphone. Although select tasks required assistance, all participants were eventually able to complete all tasks. Overall, ePROM usability did not correlate with age, sex, country, or disease state. Feedback on the general usability of both electronic devices was largely positive, and most participants (n = 11; 92%) were willing to use them. Participants were generally pleased with the training modules offered on both devices, describing the training as sufficient, straightforward, and helpful. In total, 25 usability issues were identified, which fell into three categories: incomplete instructions, unclear language, and insufficient technical/visual design. Participants provided feedback on how to enhance device usability.

Conclusions
The results suggest that older people can confidently use a tablet or smartphone for ePROM completion, particularly with appropriate training. ePROMs should be designed with the needs of the target patient population in mind. These results can be leveraged to improve clinical data recording, optimize device usability, and enhance the user experience for older people and those with functional or physical limitations.

Graphical abstract
Improvements in quality of life of patients with multiple sclerosis receiving alemtuzumab in clinical practice: the LEMVIDA study
Authors: Meca-Lallana José Eustasio , Eichau Sara , Casanova Bonaventura , Rodríguez Elena Álvarez , Pato Antonio , Forner Mireia , Toledo Baldo 
Published: December 2024

Abstract
Background
Alemtuzumab is a humanized monoclonal antibody approved for the treatment of relapsing-remitting multiple sclerosis (RRMS). Its efficacy and safety have been widely demonstrated in clinical trials, but experience from real-world cohorts is also needed to support its clinical use. Quality of life (QoL) outcomes are an important complement to the clinical benefits of treatment, offering a patient-centered perspective on how the drug contributes to general well-being. In this line we aimed to evaluate the QoL of patients treated with alemtuzumab in clinical practice.

Methods
This prospective 3-year multicenter study was carried out in adult patients diagnosed with RRMS who had started alemtuzumab according to clinical practice within 8 weeks before inclusion. The primary endpoint was the change in QoL over three years of treatment with alemtuzumab using the 29-item Multiple Sclerosis Impact Scale (MSIS-29). Secondary endpoints included changes from baseline in the 21-item Modified Fatigue Impact Scale (MFIS-21), Beck Depression Inventory (BDI-II), Symbol Digit Modalities Test (SDMT, oral version) and Work Productivity. Disability worsening was also assessed based on the Expanded Disability Status Scale (EDSS), along with the annualized relapse rate (ARR) and radiological activity.

Results
A cohort of 165 patients was analyzed (mean age 38.6 years, mean disease duration 8.5 years, mean EDSS score 3.3). MSIS-29 physical domain scores decreased significantly from baseline by a mean of 7.2 ± 1.8 points at year 1, 6.4 ± 2.2 at year 2 and 5.6 ± 2.3 at year 3 (p < 0.05 in all cases). Similarly, MSIS-29 psychological domain scores decreased significantly by a mean of 7.9 ± 2.4 points at year 1, 12.8 ± 2.9 at year 2 and 13.2 ± 3.0 at year 3 (p < 0.05 in all cases). Significant reductions from baseline were also evidenced in MFIS-21 and BDI-II scores, while SDMT scores remained unchanged. During the 3 years on alemtuzumab, the ARR was 0.15, representing an 83% reduction from the 2 years before initiation. At 3 years, 81.5% of patients were free from radiological activity and 87% were free from disability worsening.

Conclusions
These results indicate early and substantial improvements in patients’ perception of their QoL and functioning with alemtuzumab that were sustained over three years.
Development of a standard set of key work-related outcomes for use in practice for patients with cardiovascular disease: a modified Delphi study
Authors: Hagendijk Marije E. , Zipfel Nina , Hoving Jan L. , Melles Marijke , van der Wees Philip J. , van der Burg-Vermeulen Sylvia J. 
Published: December 2024

Abstract
Background
To facilitate the maintenance or resumption of participation in work for patients with cardiovascular disease (CVD), there is a need for high-quality work-focused healthcare. According to the concept of value-based healthcare, quality of care can be enhanced by understanding the outcomes that matter most to patients. However, a major challenge in assessing quality of work-focused healthcare in practice is the lack of consensus on which work-related outcomes should be measured.

Objective
The objective of this study was to identify a standard set of key work-related outcomes for patients with CVD to be used in practice of work-focused healthcare in the Netherlands, including standardised outcome measures and associated case mix factors. This standard set is intended to assist occupational and other health professionals in delivering work-focused healthcare that meets a patient’s individual needs regarding work participation, and to enhance patients’ engagement in their own work-focused care process.

Methods
A 2-round RAND-modified Delphi process was conducted. The process included literature searches, consecutive research team meetings, and several meetings and rounds of voting by a working group. The working group consisted of patients with CVD (n = 6) and health professionals representing different stakeholders (n = 11) involved in work-focused healthcare for this patient population in the Netherlands. Consensus was reached over four phases: (1) establishing the scope of the standard set and defining the population, (2) prioritising and defining the outcome domains, (3) selecting the outcome measures for the most important domains, including clinical data and patient-reported data, and (4) selecting and defining case mix factors.

Results
A 23-item patient-reported questionnaire was developed, called the Value@WORK-Q23, including questions on nine work-related outcome domains considered most important for patients with CVD: (1) work participation, (2) physical work ability, (3) mental work ability, (4) suitable work, (5) support from the work environment, (6) flexibility of the work environment, (7) communication with the patient, (8) person-centredness, and (9) interdisciplinary communication. In addition, nine case mix variables was selected, comprising demographic-, disease-, and work factors.

Conclusions
The Value@WORK-Q23 provides guidance on measuring the most important work-related outcomes for patients with CVD. Using this work-related set in practice, in addition to existing disease-specific standard sets for CVD may facilitate the provision of high-value work-focused healthcare for this patient population.
Virulence of Jordanian indigenous Beauveria bassiana isolates as entomopathogenic fungi against red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)
Authors: Irshaid Leena A. , Sweiss Mais A. , Hasan Hazem S. , Dahi Hassan F. , Al-Awaisheh Heba A. , Al-Rajabi Danea A. 
Published: December 2024

Abstract
Background
Red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae), has recently become a significant threat to date palm trees in Jordan and other regions around the world. This destructive pest causes significant economic losses by boring into palm trunks, leading to tree death. Among the different pest management methods within integrated pest management (IPM) programs, biological control utilizing microorganisms has emerged as a promising sustainable approach. Of particular interest is the entomopathogenic fungus Beauveria bassiana which has shown significant efficacy against various insect pests and could be promising.

Results
Three local isolates of B. bassiana were obtained from soil samples in the Al-Balqa governorate, Jordan. These isolates were identified using morphological and molecular techniques. The virulence of these isolates against RPW larvae and adults was evaluated under laboratory conditions, with isolate BA1 approved to be the most effective, causing 100% mortality rates of wax moth larvae and 90% in RPW larvae, with about 60% mortality of RPW adults. Comparative studies underscored the variability in virulence among different B. bassiana isolates which were influenced by factors such as hydrophobicity and enzymatic activity.

Conclusions
The findings suggest that local B. bassiana isolates hold potential as an environmentally safe strategy for IPM in date palm cultivation, contributing to sustainable pest control practices for RPW.
Himalayan fermented beverages and their therapeutic properties with scientific validations: a comprehensive review
Authors: Majumder Soumya , Bhattacharya Malay 
Published: December 2024

AbstractThe Himalayan ethnoecology revolves around a balanced relationship between harsh mountain environment and the diverse lifestyles of its ethnic communities, where traditional knowledge on medicinal plants and fermented foods plays important role in their survival strategies. At high-altitudinal cold temperature and hypoxic condition, Himalayan tribal communities opt for easily digestible pre-digested foods rich in nutrients. Lack of suitable conditions for agriculture, the remoteness of the settlements, poor transportation systems, and limited availability of raw materials throw challenges to the agriculture and food production in the Himalayas which necessitate food preservation to extend shelf life. Thus, fermentation process is carried out to extend the shelf life of a food rather than just alcohol production. Chhang, soor, tongba, jaanr, raksi, apong, yu, zutho, judima, chu, qingke, ara, etc., are traditionally fermented beverages consumed in the Himalayan regions for relaxation which also offer health benefits. These ethnic beverages are often fortified with ethnomedicinal natural products native to the Himalayas which help inhabitants and tourists in coping with altitude-related stresses and acclimatizing to the region's climate. This review work begins with a background unfolding various altitudinal stresses on human health in Himalaya’s perspective and continues with an investigation into the scientifically proven benefits of traditional fermented beverages. There is a pile of document that reports surveyed secondary data on identification of region specific starters and beverages, ethnobotanic preparations and ethnomedicinal claims of antioxidant, anti-inflammatory, antimicrobial, gastrointestinal-protective, rejuvenating and altitude-sickness healing properties. This review article identified the limited volume research on nutraceutical values of the Himalayan fermented beverages and described scientific validations of the ethnomedicinal claims through biochemical characterization, ethno-microbiology and metabolomics.