bluuebunny/crossref_metadata_2025_split huggingface dataset:
Setting maximum levels for lead in game meat in EC regulations: An adjunct to replacement of lead ammunition
Authors: Thomas Vernon G. , Pain Deborah J. , Kanstrup Niels , Green Rhys E. 
Published: May 2020

AbstractEach year, hunters from 12 of the 27 European Union (EU) countries and the UK shoot over 6 million large game mammals, 12 million rabbits and hares and over 80 million birds. They support an international game meat market worth over 1.1 thousand million Euros. Animals shot with lead ammunition frequently contain lead fragments in the carcass which contaminate meals made from game meat with concentrations of lead substantially above the maximum allowable level (ML) set by European Commission Regulation EC1881/2006 for meat from domesticated animals. This poses a health risk to frequent consumers of wild-shot game meat, with children and pregnant women being particularly vulnerable. Total replacement of lead rifle and shotgun ammunition with available non-toxic alternatives is needed for all hunting in EU nations to prevent exposure of humans and wildlife to ammunition-derived lead and to allow the depletion of the long-term environmental legacy of lead from spent ammunition. We propose that EC1881/2006 is amended to incorporate an ML for game meats as a supplementary measure to the replacement of lead ammunition. This would harmonise food safety standards for lead in meats traded across and imported into the EU.
CMIP6 Models Predict Significant 21st Century Decline of the Atlantic Meridional Overturning Circulation
Authors: Weijer W. , Cheng W. , Garuba O. A. , Hu A. , Nadiga B. T. 
Published: May 2020

AbstractWe explore the representation of the Atlantic Meridional Overturning Circulation (AMOC) in 27 models from the CMIP6 multimodel ensemble. Comparison with RAPID and SAMBA observations suggests that the ensemble mean represents the AMOC strength and vertical profile reasonably well. Linear trends over the entire historical period (1850–2014) are generally neutral, but many models exhibit an AMOC peak around the 1980s. Ensemble mean AMOC decline in future (SSP) scenarios is stronger in CMIP6 than CMIP5 models. In fact, AMOC decline in CMIP6 is surprisingly insensitive to the scenario at least up to 2060. We find an emergent relationship among a majority of models between AMOC strength and 21st century AMOC decline. Constraining this relationship with RAPID observations suggests that the AMOC might decline between 6 and 8 Sv (34–45%) by 2100. A smaller group of models projects much less AMOC weakening of only up to 30%.
Extending the Global Mass Change Data Record: GRACE Follow‐On Instrument and Science Data Performance
Authors: Landerer Felix W. , Flechtner Frank M. , Save Himanshu , Webb Frank H. , Bandikova Tamara , Bertiger William I. , Bettadpur Srinivas V. , Byun Sung Hun , Dahle Christoph , Dobslaw Henryk , Fahnestock Eugene , Harvey Nate , Kang Zhigui , Kruizinga Gerhard L. H. , Loomis Bryant D. , McCullough Christopher , Murböck Michael , Nagel Peter , Paik Meegyeong , Pie Nadege , Poole Steve , Strekalov Dmitry , Tamisiea Mark E. , Wang Furun , Watkins Michael M. , Wen Hui‐Ying , Wiese David N. , Yuan Dah‐Ning 
Published: May 2020

AbstractSince June, 2018, the Gravity Recovery and Climate Experiment Follow‐On (GRACE‐FO) is extending the 15‐year monthly mass change record of the GRACE mission, which ended in June 2017. The GRACE‐FO instrument and flight system performance has improved over GRACE. Better attitude solutions and enhanced pointing performance result in reduced fuel consumption and gravity range rate post‐fit residuals. One accelerometer requires additional calibrations due to unexpected measurement noise. The GRACE‐FO gravity and mass change fields from June 2018 through December 2019 continue the GRACE record at an equivalent precision and spatiotemporal sampling. During this period, GRACE‐FO observed large interannual terrestrial water variations associated with excess rainfall (Central US, Middle East), drought (Europe, Australia), and ice melt (Greenland). These observations are consistent with independent mass change estimates, providing high confidence that no intermission biases exist from GRACE to GRACE‐FO, despite the 11‐month gap. GRACE‐FO has also successfully demonstrated satellite‐to‐satellite laser ranging interferometry.
Gene expression regulation by CDK12: a versatile kinase in cancer with functions beyond CTD phosphorylation
Authors: Choi Seung Hyuk , Kim Seongjae , Jones Katherine A. 
Published: May 2020

AbstractCyclin-dependent kinases (CDKs) play critical roles in cell cycle progression and gene expression regulation. In human cancer, transcription-associated CDKs can activate oncogenic gene expression programs, whereas cell cycle-regulatory CDKs mainly induce uncontrolled proliferation. Cyclin-dependent kinase 12 (CDK12) belongs to the CDK family of serine/threonine kinases and has been recently found to have multiple roles in gene expression regulation and tumorigenesis. Originally, CDK12 was thought to be one of the transcription-associated CDKs, acting with its cyclin partner Cyclin K to promote the phosphorylation of the C-terminal domain (CTD) of RNA polymerase II and induce transcription elongation. However, recent studies have demonstrated that CDK12 also controls multiple gene expression processes, including transcription termination, mRNA splicing, and translation. Most importantly, CDK12 mutations are frequently found in human tumors. Loss of CDK12 function causes defective expression of DNA damage response (DDR) genes, which eventually results in genome instability, a hallmark of human cancer. Here, we discuss the diverse roles of CDK12 in gene expression regulation and human cancer, focusing on newly identified CDK12 kinase functions in cellular processes and highlighting CDK12 as a promising therapeutic target for human cancer treatment.
Stringent control of the RNA-dependent RNA polymerase translocation revealed by multiple intermediate structures
Authors: Wang Meihua , Li Rui , Shu Bo , Jing Xuping , Ye Han-Qing , Gong Peng 
Published: May 2020

AbstractEach polymerase nucleotide addition cycle is associated with two primary conformational changes of the catalytic complex: the pre-chemistry active site closure and post-chemistry translocation. While active site closure is well interpreted by numerous crystallographic snapshots, translocation intermediates are rarely captured. Here we report three types of intermediate structures in an RNA-dependent RNA polymerase (RdRP). The first two types, captured in forward and reverse translocation events, both highlight the role of RdRP-unique motif G in restricting the RNA template movement, corresponding to the rate-limiting step in translocation. By mutating two critical residues in motif G, we obtain the third type of intermediates that may mimic the transition state of this rate-limiting step, demonstrating a previously unidentified movement of the template strand. We propose that a similar strategy may be utilized by other classes of nucleic acid polymerases to ensure templating nucleotide positioning for efficient catalysis through restricting interactions with template RNA.
Impact of warming and reduced precipitation on morphology and chlorophyll concentration in peat mosses (Sphagnum angustifolium and S. fallax)
Authors: Rastogi Anshu , Antala Michal , Gąbka Maciej , Rosadziński Stanisław , Stróżecki Marcin , Brestic Marian , Juszczak Radosław 
Published: May 2020

AbstractPeatlands are one of the most important ecosystems due to their biodiversity and abundant organic compounds; therefore, it is important to observe how different plant species in peatlands react to changing environmental conditions. Sphagnum spp. are the main component of peatlands and are considered as the creator of conditions favorable for carbon storage in the form of peat. Sphagnum angustifolium and Sphagnum fallax are taxonomically very close species. To examine their adaptability to climate change, we studied the morphology and pigment content of these two species from environmental manipulation sites in Poland, where the environment was continuously manipulated for temperature and precipitation. The warming of peat was induced by using infrared heaters, whereas total precipitation was reduced by a curtain that cuts the nighttime precipitation. Morphology of S. angustifolium stayed under climate manipulation relatively stable. However, the main morphological parameters of S. fallax were significantly affected by precipitation reduction. Thus, this study indicates S. angustifolium is better adapted in comparison to S. fallax for drier and warmer conditions.
CCR3 antagonist protects against induced cellular senescence and promotes rejuvenation in periodontal ligament cells for stimulating pulp regeneration in the aged dog
Authors: Zayed Mohammed , Iohara Koichiro , Watanabe Hideto , Nakashima Misako 
Published: May 2020

AbstractPulp regeneration after transplantation of mobilized dental pulp stem cells (MDPSCs) declines in the aged dogs due in part to the chronic inflammation and/or cellular senescence. Eotaxin-1/C-C motif chemokine 11 (CCL11) is an inflammation marker via chemokine receptor 3 (CCR3). Moreover, CCR3 antagonist (CCR3A) can inhibit CCL11 binding to CCR3 and prevent CCL11/CCR3 signaling. The study aimed to examine the effect of CCR3A on cellular senescence and anti-inflammation/immunomodulation in human periodontal ligament cells (HPDLCs). The rejuvenating effects of CCR3A on neurite extension and migratory activity to promote pulp regeneration in aged dog teeth were also evaluated.In vivo, the amount of regenerated pulp tissues was significantly increased by transplantation of MDPSCs with CCR3A compared to control without CCR3A.In vitro, senescence of HPDLCs was induced afterp-Cresol exposure, as indicated by increased cell size, decreased proliferation and increased senescence markers,p21andIL-1β. Treatment of HPDLCs with CCR3A prevented the senescence effect ofp-Cresol. Furthermore, CCR3A significantly decreased expression of CCL11, increased expression of immunomodulatory factor, IDO, and enhanced neurite extension and migratory activity. In conclusion, CCR3A protects againstp-Cresol-induced cellular senescence and enhances rejuvenating effects, suggesting its potential utility to stimulate pulp regeneration in the aged teeth.
A biomathematical model of human erythropoiesis and iron metabolism
Authors: Schirm Sibylle , Scholz Markus 
Published: May 2020

AbstractAnaemia therapy or perisurgical support of erythropoiesis often require both, EPO and iron medication. However, excessive iron medication can result in iron overload and it is challenging to control haemoglobin levels in a desired range. To support this task, we develop a biomathematical model to simulate EPO- and iron medication in humans. We combine our previously established model of human erythropoiesis including comprehensive pharmacokinetic models of EPO applications with a newly developed model of iron metabolism including iron supplementation. Equations were derived by translating known biological mechanisms into ordinary differential equations. Qualitative model behaviour is studied in detail considering a variety of interventions such as bleeding, iron malnutrition and medication. The model can explain time courses of erythrocytes, reticulocytes, haemoglobin, haematocrit, red blood cells, EPO, serum iron, ferritin, transferrin saturation, and transferrin under a variety of scenarios including EPO and iron application into healthy volunteers or chemotherapy patients. Unknown model parameters were determined by fitting the predictions of the model to time series data from literature. We demonstrate how the model can be used to make predictions of untested therapy options such as cytotoxic chemotherapy supported by iron and EPO. Following our ultimate goal of establishing a model of anaemia treatment in chronic kidney disease, we aim at translating our model to this pathological condition in the near future.
Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein
Authors: Zhang Jun , Peng Ching-An 
Published: May 2020

AbstractCD200 is known as an anti-inflammatory transmembrane glycoprotein in the immunoglobulin superfamily. CD200 interacts with its receptor CD200R which is highly expressed on myeloid cells such as macrophages and neutrophils. CD200-CD200R interaction has known to reduce macrophage activation and chronic inflammation. To harness the immunomodulatory property of CD200 for surface modification, CD200-streptavidin fusion protein was expressed from bacteria transformed with pET20b plasmid encoded with CD200 extracellular domain and core streptavidin. The purified CD200-SA protein was bound to biotin-coated fluorescent polystyrene particles of various sizes ranging from 0.15 to 2 µm. THP-1 macrophages were cultivated with CD200-modified micro/nanoparticles in comparison with controls. Our results showed that both nano- and micro-sized particles decorated with CD200 decreased phagocytosis activities of THP-1 macrophages. Such diminution of phagocytosis was examined to be associated with downregulation of Toll-like receptor 4 (TLR4) expression on the surface of macrophages. Moreover, THP-1 macrophages treated with CD200-coated particles decreased the secretion of tumor necrosis factor-α (TNF-α).
A Portable Smartphone-linked Device for Direct, Rapid and Chemical-Free Hemoglobin Assay
Authors: Lee Junho , Song Jaewoo , Choi Jun-Ho , Kim Soocheol , Kim Uihan , Nguyen Van-Thuan , Lee Jong-Seok , Joo Chulmin 
Published: May 2020

AbstractWe describe the development and clinical evaluation of an automated smartphone-linked sensor capable of chemical-free, quantitative measurement of hemoglobin concentration ([Hb]) in whole blood samples. We have demonstrated that our sensor could analyze an unprocessed blood specimen with a mean processing time of <8 s and provided the [Hb] results with ~99% accuracy against a reference hematology analyzer with coefficient of variation (CV) of 1.21% measured at [Hb] = 11.2 g/dL. Its diagnostic capability for anemia was evaluated by measuring [Hb] of 142 clinical blood specimens and comparing the results with those from an automated hematology analyzer (ADVIA 2120i, Siemens AG, Germany) and a portable hemoglobinomteter (Hb201+, Hemocue, Sweden). The sensor yielded comparable sensitivities and specificities of 87.50% and 100.00% for males, and 94.44% and 100.00% for females, respectively, for anemic detection. The results suggested that our optical sensor based on the intrinsic photothermal response of Hb molecules and advances in consumer electronics, particularly smartphone capabilities, enables a direct, chemical-free [Hb] assay accessible to people in both developed and developing countries.
Dissection-enabled scaffold-assisted resection (DeSCAR): a novel technique for resection of residual or non-lifting gastrointestinal neoplasia of the colon, expanded experience and follow-up
Authors: Villa Edward , Stier Matthew , Donboli Kianoush , Chapman Christopher Grant , Siddiqui Uzma D. , Waxman Irving 
Published: May 2020

Abstract
Background and study aims Colonic lesions may not be amenable to conventional endoscopic mucosal resection (EMR) due to previous manipulation, submucosal invasion, or lesion flatness. In 2018, we described Dissection-enabled Scaffold Assisted Resection (DeSCAR) to be safe for the endoscopic resection of non-lifting or residual colonic lesions 1 In this study, we expand our original cohort to describe our expanded experience with patients undergoing DeSCAR and assess the efficacy, safety, and feasibility of DeSCAR for endoscopic resection of non-lifting or residual colonic lesions.
Patients and methods We retrospectively reviewed 57 patients from 2015–2019 who underwent DeSCAR for colonic lesions with incomplete lifting and/or previous manipulation. Cases were reviewed for location, prior manipulation, rates of successful resection, adverse events, and endoscopic follow up to assess for residual lesions.
Results Fifty-seven lesions underwent DeSCAR. Of the patients, 51 % were female, and average patient age was 69 years. Lesions were located in the cecum (n = 16), right colon (n = 27), left colon (n = 10), and rectum (n = 4). Average lesion size was 27.7 mm. Previous manipulation occurred in 54 cases (72 % biopsy, 44 % resection attempt, 18 % intralesional tattoo). The technical success rate for resection of non-lifting lesions was 98 %. There were two delayed bleeding episodes (one required endoscopic intervention) and one small perforation (managed by endoscopic hemoclip closure). Endoscopic follow up was available in 31 patients (54 %) with no residual adenoma in 28 patients (90 % of those surveilled).
Conclusions Our expanded experience with DeSCAR demonstrates high safety, feasibility, and effectiveness for the endoscopic management of non-lifting or residual colonic lesions.
Phenotypic and Genotypic Analysis of a Hereditary Antithrombin Deficiency Pedigree Due to a Novel SERPINC1 Mutation (p.Met281Thr)
Authors: Liu Siqi , Luo Shasha , Yang Lihong , Wang Mingshan , Jin Yanhui , Li Xiaolong , Xu Qiyu 
Published: May 2020

AbstractAntithrombin (AT) is one of the physiological anticoagulants that are mainly synthesized in the liver. As a protease inhibitor belonging to the serpin superfamily, AT is able to inactivate thrombin and inhibit activated coagulation factors IX, X, XI, and XII (FIXa, FXa, FXIa, and FXIIa).1 Moreover, it has been found that AT can inhibit activated FVII (FVIIa) by accelerating dissociation of FVIIa–tissue factor complex and preventing it from recombining.2 The AT gene (SERPINC1), located on chromosome 1 at q23.1–23.9 and spreads 13.5 kb, is composed of seven extrons and six introns.3 Hereditary AT deficiency is a rare thrombotic disorder caused by defects in SERPINC1 gene.4 It is inherited in an autosomal-dominant manner with an incidence of roughly 0.02 to 0.25% in the general population and 2 to 5% in patients with a history of venous thromboembolism (VTE).1
5 The most common thrombotic manifestations of patients with AT deficiency are VTEs, and their risks of VTE are approximately 20 times higher than those of nondeficient individuals.6 And the consequences of thrombophilia caused by AT deficiency are more severe than those of protein C and S protein deficiency,2 so it should be given sufficient attention.
Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review
Authors: Houdkova Marketa , Kokoska Ladislav 
Published: May 2020

AbstractThis review summarizes data on the in vitro antimicrobial effectiveness of volatile agents of plant origin and in vitro methods for evaluating their activity in the vapour phase. As a result of literature analysis, the antimicrobial efficacy of vapours from 122 different plant species and 19 pure compounds examined in 61 studies using different in vitro tests against a broad spectrum of microorganisms was identified and summarized. In addition, 11 different techniques found in the literature are described in detail. An original classification of methods based on the solid and liquid matrix volatilization principle is proposed because carrier medium/matrix selection is crucial for the volatilization of any agents tested. This review should be useful for medicinal, pharmaceutical, food, and agricultural experts working in areas related to the management of infectious diseases (especially respiratory and skin infections), food preservation (active packaging), and protection of agriculture products (controlled atmosphere). It may also stimulate the interest of pharmaceutical, cosmetic, food, and agriculture industries in the research and development of new antimicrobial agents of natural origin. Since several original apparatuses previously developed for antimicrobial susceptibility testing in the vapour phase are described in this review, labware manufacturers may also be interested in this topic. The review also provides specific guidelines and recommendations for researchers studying the antimicrobial activity of volatile agents. The article will therefore appeal to communities of industrial stakeholders, pharmacists, physicians, food experts, agriculturists, and researchers in related areas such as pharmacology, medicinal chemistry, microbiology, natural product chemistry, food preservation and plant protection.
Surgery as a Component of Multimodality Care for Known Stage IIIA-N2 Non-small Cell Lung Cancer
Authors: Bograd Adam J. , Vallières Eric 
Published: May 2020

AbstractLocally advanced, stage IIIA-N2 Non-small cell lung cancer (NSCLC) represents a heterogeneous patient population. Considerable controversy exists as to the optimal management of these patients. Local therapy alone with a single modality, namely surgery or radiation, is associated with high recurrence rates and low overall survival. Consequently, multimodality treatment (chemotherapy, radiotherapy, with or without the utilization of surgery) has developed as a means of both local and systemic control for patients with stage IIIA-N2 NSCLC, and has led to improved overall survival. While it is clear that multimodality therapy is beneficial, the ideal composition of the treatment regimen remains a work in progress. As a result, the optimal management of patients with stage IIIA-N2 NSCLC is widely debated, as is the role for surgery in a multimodality treatment regimen. Herein, we review the current literature supporting multimodality therapy for stage IIIA-N2 NSCLC with an emphasis on the data supporting the role of surgery in resectable and potentially resectable patients.
Update on Lung Cancer Screening
Authors: Brownlee Andrew R. , Donington Jessica S. 
Published: May 2020

AbstractOver the past 10 years, there has been substantial progress in the study and implementation of lung cancer screening using low-dose computed tomography (LDCT). The National Lung Screening Trial, the recently reported NELSON (NEderlands-Leuvens Longkanker Screenings ONderzoek) trial, and other European trials provide strong evidence for the efficacy of LDCT to reduce lung cancer mortality. This has resulted in the United State's Preventative Task Force and numerous professional medical societies adopting lung cancer screening recommendations. Despite the general acceptance of the positive effect of screening, low adoption and implementation rates remain nationally. In this article, the authors discuss the evolution and current state of the evidence for LDCT screening for lung cancer. The authors will also review the associated risks, cost, and challenges of implementation of an LDCT screening program.