bluuebunny/crossref_metadata_2025_split huggingface dataset:
Title: The prognostic significance of survivin expression in patientswith HNSCC: a systematic review and meta-analysis
Authors: Zhou Liu-qing , Hu Yao , Xiao Hong-jun 
Published: January 2021

Abstract
Background: Survivin has been recently identified as a promising novel therapeutic target and prognostic marker in different types of cancer. Here we conducted a comprehensive meta-analysis to better clarify they the precise prognostic and diagnostic value of survivin in head and neck squamous cell carcinoma (HNSCC). Methods: Database of PubMed (Medline), Embase, and Web of Science were systematically searched for related published literature up to September 2020. Pooled hazards ratios (HRs) and related 95% confidence intervals (CIs) were used to estimate the association of survivin expression and survival outcomes in HNSCC patients. Results: Twenty eight studies with 4891 patients were finally included in this meta-analysis, the pooled analysis indicated that the survivin expression was significantly correlated with poorer overall survival (OS) (HR, 2.02; 95% CI, 1.65-2.47, P<0.001), and poorer disease-free survival (DFS)/ disease-specific survival (DSS) (HR = 2.03, 95%CI: 1.64-2.52, P<0.001; HR = 1.92, 95%CI: 1.41-2.60, P<0.001, receptively). In addition, we observed the similar results in subgroup analysis stratified by different cancer types, such as laryngeal squamous cell carcinoma (LSCC) (HR = 1.35, 95%CI: 1.05-1.74, P<0.001), oral squamous cell carcinomas (OSCC) (HR = 2.45, 95%CI: 1.89-3.17, P<0.001), nasopharyngeal carcinoma (NPC) (HR = 2.53, 95%CI: 1.76-3.62, P<0.001) and HNSCC (HR = 1.52, 95%CI: 1.25-1.86, P<0.001).Conclusions: Our results suggested that survivin is predictors of worse prognosis in HNSCC patients. Hence, survivin is a potential therapeutic target for HNSCC.
Comparisons of Vitreal Angiogenic, Inflammatory, Profibrotic Cytokines, and Chemokines Profile Between Patients With Epiretinal Membrane and Macular Hole
Authors: Chen Lu , Zhang Weiwei , Xie Ping , Ji Jiangdong , Qian Huiming , Yuan Songtao , Liu Qinghuai , Hu Zizhong 
Published: January 2021

Abstract
Objectives: Idiopathic epiretinal membrane (iERM) or idiopathic macular hole (iMH) is frequently used as “healthy” control in comparison of vitreous cytokines with other vitreoretinal diseases. This study aimed to investigate if there is difference in vitreal cytokines expression between patients with iERM and iMH.Methods: In this prospective study, all subjects received standard pars plana vitrectomy surgery and 0.5 ml of native vitreous sample was extracted during the vitrectomy. Luminex technology and enzyme-linked immunosorbent assay were used to profile the concentration of 52 classic angiogenic, inflammatory, and profibrotic cytokines, and chemokines. Statistical analyses were performed by Mann-Whitney U test, followed with multiple comparisons by Bonferroni correction.Results: Vitreal samples from 13 iERM and from 24 iMH were studied. Of the 52 tested cytokines, 41 were similar in expression, 5 were under the detection limit, while 6 cytokines ((MMP-8, Eotaxin, MIP-1a, RANTES, TGF-β2, IL-4)) were differently expressed between two groups (p < 0.05). Nevertheless, these significances disappeared after adjustment of Bonferroni correction.Conclusion: The tested cytokines showed similar expression between iERM and iMH patients. This indicates that eyes with iERM or iMH can be together served as “healthy” controls.
Peripheral Immune Profile of Children With Talaromyces Marneffei Infections: A Retrospective Analysis of 21 Cases
Authors: Zeng Qiang , Jin Yingkang , Yin Genquan , Yang Diyuan , Li Wenyan , Shi Tingting , Lu Gen , Huang Li , Fan Huifeng 
Published: January 2021

Abstract
BackgroundTalaromyces (formerly Penicillium) marneffei (T. marneffei) is an opportunistic pathogen that infects immunodeficient and immunocompromised children. The aim of the study is to determine the clinical features and peripheral immune state of Talaromyces marneffei (T. marneffei) infections in children for early detection and diagnosis.MethodsWe retrospectively reviewed 21 pediatric patients who were diagnosed with T. marneffei infections and were followed up in the Guangzhou Women and Children’s Medical Center from January 2010 to January 2020. For each patient, we collected and analyzed clinical characteristics, peripheral immunological results, genetic tests, complications and prognosis.ResultsCommon clinical features of the patients included fever (20/21, 95.24%), cough (17/21, 80.95%) and hepatomegaly (17/21, 80.95%). Severe complications included septic shock (12/21, 57.14%), hemophagocytic lymphohistiocytosis (HLH) (11/21, 52.38%), acute respiratory distress syndrome (ARDS) (10/21, 47.62%), multiple organ dysfunction syndrome (MODS) (9/21, 42.86%), and disseminated intravascular coagulation (DIC) (7/21, 33.33%). Eleven children (11/21, 52.38%) eventually died of T. marneffei infections. All patients were HIV negative. Seven cases revealed reduced antibody levels, especially IgG. Higher levels of IgE were detected in 9 cases with an obvious increase in two patients. Ten patients showed decreased complement C3 levels, some of whom had low C4 levels. Three patients displayed decreased absolute T lymphocyte counts, including the CD 4+ and CD 8+ subsets. A reduction in NK cells was present in most patients. No patient had positive nitro blue tetrazolium (NBT) test results. Nine patients were assessed using gene tests. Of the cases, one case had no disease-specific gene mutation. Four children had confirmed hyperimmunoglobulin M syndrome (HIGM) with CD40LG variation, one case had severe combined immunodeficiency (SCID), and one case had hyper-IgE syndrome (HIES). One patient was identified as having a heterozygous mutation in STAT3 gene; however, he showed no typical clinical manifestations of HIES at his age. One patient had a mutated COPA gene with uncertain pathogenic potential. Another patient was diagnosed with HIES that depended on her clinical features and the National Institutes of Health (NIH) scoring system.ConclusionT. marneffei infections in HIV-negative children induced severe systemic complications and poor prognosis. Children with T. marneffei infections commonly exhibited abnormal immunoglobulin levels in peripheral blood, particularly decreased IgG or increased IgE levels, further suggesting possible underlying PIDs in these patients.
Towards Implementing Exercise Into the Prostate Cancer Care Pathway: Development of a Theory and Evidence-Based Intervention to Change Healthcare Professional Behaviour
Authors: Turner Rebecca , Arden Madelynne , Reale Sophie , Sutton Eileen , Taylor Stephanie , Bourke Liam , Greenfield Diana , Morrissey Dylan , Brown Janet , Doherty Patrick , Rosario Derek , Steed Liz , N/A on behalf of the STAMINA co-investigators 
Published: January 2021

Abstract
BackgroundTwice-weekly supervised aerobic and resistance exercise for 12 weeks reduces fatigue and improves quality of life in men on Androgen Deprivation Therapy for prostate cancer. Despite the National Institute for Health and Care Excellence (NICE) proposing this as standard of care, it does not routinely take place in practice. Healthcare professionals are in a prime position to deliver and integrate these recommendations. A change in the behaviour of clinical teams is therefore required.In this paper, we describe the development of a training package for healthcare professionals using theory and evidence to promote delivery of such recommendations as standard care.MethodsThe intervention development process was guided by the Medical Research Council guidance for complex interventions and the Behaviour Change Wheel. Target behaviours were identified from the literature and thirty-five prostate cancer care healthcare professionals were interviewed to understand influences on these behaviours. The Theoretical Domains Framework was used to identify theoretical constructs for change. Behaviour change techniques were selected based on theory and evidence and were translated into intervention content. The intervention was refined with the input of stakeholders including healthcare professionals, patients, and exercise professionals in the form of rehearsal deliveries, focus groups and a workshop.ResultsSeven modifiable healthcare professional target behaviours were identified to support the delivery of the NICE recommendations including identifying eligible patients suitable for exercise, recommending exercise, providing information, exercise referral, providing support and interpret and feedback on progress. Ten domains from the Theoretical Domain’s Framework were identified as necessary for change, including improving knowledge and skills, addressing beliefs about consequences, and targeting social influences. These were targeted through twenty-two behaviour change techniques delivered in a half-day, interactive training package. Based on initial feedback from stakeholders, the intervention was refined in preparation for evaluation.ConclusionsWe designed an intervention based on theory, evidence, and stakeholder feedback to promote and support the delivery of NICE recommendations. Future work will aim to test this training package in a multi-centre randomised trial. If proven effective, the development and training package will provide a template for replication in other clinical populations, where exercise has proven efficacy but is insufficiently implemented.Trial registrationN/A
Imaging biological tissue with high-throughput single-pixel compressive holography
Authors: Daixuan Wu , Jiawei Luo , Guoqiang Huang , Feng Yuanhua , Xiaohua Feng , Yuecheng Shen , Li Zhao-Hui 
Published: January 2021

Abstract
Single-pixel holography (SPH) is capable of generating holographic images with rich spatial information by employing only a single-pixel detector. Thanks to the relatively low dark-noise production, high sensitivity, large bandwidth, and cheap price of single-pixel detectors in comparison to pixel-array detectors, SPH is becoming an attractive imaging modality at wavelengths where pixel-array detectors are not available or prohibitively expensive. Moreover, SPH is particularly advantageous when imaging through scattering media or in scarce illumination with compressive sensing. In the current practice of SPH, the throughput of the system is mainly limited by the phase-encoded illumination and the ways to realize phase stepping. In this work, we developed a high-through single-pixel compressive holography, achieving a space-bandwidth-time product (SBP-T) of 41,667 pixels/s. This result indicates that by using a single-pixel detector, information of holographic images containing up to 65,536 pixels can be collected within only 3 seconds. The high-throughput was realized by enabling phase stepping naturally in time and abandoning the need for phase-encoded illumination. We further show that compressive sensing can be conveniently adapted to significantly reduce the acquisition time. Besides being high throughput, we also show that this holographic system is scalable to provide either a large field of view (~83 mm2) or a high resolution (5.8 μm × 4.3 μm). In particular, high-resolution holographic images of a piece of rat tail were presented, exhibiting rich information of mussel, cortical bone, and cancellous bone. Given that microscopic images of biological tissue has rarely been explored in the current practice of SPH, we anticipate the developed high-throughput SPH is promising to nourish the development of multi-spectrum imaging by providing high-quality holographic images for biological tissues.
Preoperative Factors Predicting the Severity of BMD Loss Around the Implant after Total Hip Arthroplasty
Authors: Morita Akira , Naomi Kobayashi , Choe Hyonmin , Tezuka Taro , Higashihira Shota , Inaba Yutaka 
Published: January 2021

Abstract
BackgroundStress shielding after total hip arthroplasty (THA) leads to the loss of bone mineral density (BMD) around the femoral implants, particularly in the proximal area. BMD loss around the implant is likely to occur within 1 year after THA, but its severity depends on patient characteristics. This study evaluated preoperative factors associated with the severity of zone 7 BMD loss after THA.MethodsThis retrospective cohort study included 48 patients who underwent primary THA at our hospital from October 2011 to December 2015. All patients underwent implantation of a Zweymüller-type femoral component without any postoperative osteoporosis medications. The objective variable was change in zone 7 BMD after 1 year. Factors evaluated included age, body mass index (BMI), Japanese Orthopaedic Association (JOA) score, Harris Hip Score (HHS), Canal Flare Index (CFI), and lumbar BMD on the frontal and lateral sides. Factors associated with loss of zone 7 BMD were identified by univariate and multivariate regression analyses.ResultsUnivariate regression analysis showed that CFI (P=0.003) and preoperative lumbar BMD on the frontal (P=0.003) and lateral (P<0.001) sides were significantly correlated with loss of zone 7 BMD. Multivariate regression analysis showed that CFI (P=0.014) and lumbar BMD on the lateral side (P<0.001) were independently correlated with loss of zone 7 BMD.ConclusionLower preoperative lumbar BMD on the lateral side and lower CFI were significantly associated with zone 7 BMD loss after THA. Patients with these characteristics should be carefully monitored for severe BMD loss after THA.
The Impact of Atmospheric Rivers on Rainfall in New Zealand
Authors: Shu Jingxiang , Shamseldin Asaad Y. , Weller Evan 
Published: January 2021

Abstract
This study quantifies the impact of atmospheric rivers (ARs) on rainfall in New Zealand. Using daily rainfall records from 654 rain gauges and the ERA-Interim and ERA-5 reanalysis, we investigate the contribution of ARs to the annual and seasonal rainfall totals and extreme rainfall events between 1979–2018 across the country. Results indicate that these filamentary synoptic features play an essential role in regional water resources and are responsible for many extreme rainfall events on the western side of mountainous areas and northern New Zealand. Depending on the season in these areas, 40–86% of the rainfall totals and 50–98% of extreme rainfall events are shown to be associated with ARs, with the largest contributions predominantly occurring during the summer. Furthermore, the median daily rainfall associated with ARs is 2–3 times that associated with other storms. The results of this study extend the knowledge of the critical roles of ARs on hydrology and highlight the need for further investigation of the hydrological hazards caused by ARs in New Zealand.
Restoration of deficient DNA Repair Genes Mitigates Genome Instability and Increases Productivity of Chinese Hamster Ovary Cells
Authors: Spahn Philipp , Zhang Xiaolin , Hu Qing , Hamaker Nathaniel , Hefzi Hooman , Li Shangzhong , Kuo Chih-Chung , Huang Yingxiang , Lee Jamie , Ly Peter , Lee Kelvin , Lewis Nathan 
Published: January 2021

Abstract
Chinese Hamster Ovary (CHO) cells are the primary host used for manufacturing of therapeutic proteins. However, production instability of high-titer cell lines is a major problem and is associated with genome instability, as chromosomal aberrations reduce transgene copy number and decrease protein titer. We analyzed whole-genome sequencing data from 11 CHO cell lines and found deleterious single-nucleotide polymorphisms (SNPs) in DNA repair genes. Comparison with other mammalian cells confirmed DNA repair is compromised in CHO. Restoration of key DNA repair genes by SNP reversal or expression of intact cDNAs improved DNA repair and genome stability. Moreover, the restoration of LIG4 and XRCC6 in a CHO cell line expressing secreted alkaline phosphatase mitigated transgene copy loss and improved protein titer retention. These results show for the first time that correction of key DNA repair genes yields considerable improvements in stability and protein expression in CHO, and provide new opportunities for cell line development and a more efficient and sustainable production of therapeutic proteins.
DNA Sequence and Community Structure Diversity of Multi-Year Soil Fungi in&nbsp;Grape of Xinjiang
Authors: Liu Tong , Xue Feng 
Published: January 2021

Abstract
This study is designed to understand the community structure and diversity of fungi in the rhizosphere soil of grape. As the sample for this study, the rhizosphere soil of Crimson seedless grape with different planting years was collected from Shihezi in Xinjiang to carry out high-throughput sequencing, by which the complete sequence of soil fungi DNA was identified, and accordingly, the richness and diversity index of fungi were determined. The results showed that the dominant phyla of fungi in the grape rhizosphere soil with different planting years were Ascomycota and Basidiomycota, and the dominant classes of fungi were Sordariomycetes and Dothideomycetes. Soil organic matter, total potassium, total nitrogen and available phosphorus were the main soil fertility factors affecting the abundance and diversity of soil fungal communities, among which soil organic matter had the most significant influence. In addition, the fungal diversity and richness were highest in the middle layer (20-35 cm) of the grape rhizosphere soil with 12 planting years and lowest in the lower layer (35-50 cm) of the grape rhizosphere soil with 5 planting years. Linear discriminant analysis suggested that there were more biomarkers in the vineyard rhizosphere soil with 10 planting years, which meant there were more fungal communities with significant difference in the soil, especially in the middle layer (20-35). The results of this study can provide data reference and theoretical basis for improving vineyard soil quality, evaluating soil microecological effects and improving ecological environment of vineyard soil.
Modified F Configuration in the Treatment of Pauwels Type III Femoral Neck Fracture: a Finite Element Analysis
Authors: Lin Shiyuan , Li Huizi , Tong Kai , Feng Zihang , Wang Gang , Lu Hua-Ding 
Published: January 2021

Abstract
Background: The optimal treatment of Pauwels type III femoral neck fracture in young patients remains a worldwide challenge in orthopedic surgery. Methods: Finite element models of four internal fixations were developed to treat Pauwels type III Femoral neck fracture: a: the traditional inverted triangular cannulated screw model, b: the F-technique cannulated screw model, c: the modified F-technique cannulated screw model using a fully threaded screw instead of a partially threaded distally, d: the dynamic hip screw coupled with anti-rotational screw model. Under the same conditions, finite element analyses were carried out to compare the displacement and von Mises stress distribution of four internal fixations and femurs, the maximum crack distances of the fracture surfaces, Z axis displacements of four models as well as the stress distribution in the subtrochanteric region. Results: The modified F-technique configuration resulted in a more stable fixation as compared to the other three approaches, with respect to the maximum displacement and stress peaks of femur and internal fixations, the maximum crack distances of the fracture surfaces, Z axis displacements of four configurations as well as the stress distribution in the subtrochanteric region.Conclusions: Our results suggested that modified F-technique configuration show a better performance in resisting shearing and rotational forces in treating Pauwels type III femoral neck fractures compared to those using traditional inverted triangular, the F-technique configuration or dynamic hip screw coupled with anti-rotational screw, providing a new choice for the treatment of femoral neck fractures.
An Investigation Into the Factors Inhibiting the Development of Date Crop Insurance: the Case of Saravan County
Authors: Sasouli Mohammad Reza , Karimzadeh Majid , Jamnia Abdul Rashid 
Published: January 2021

Abstract
The viability and efficiency of crop insurance policies depend on farmers’ demand and willingness for crop insurance. The present paper analyzes the insurance demand of 441 date farmers in Saravan County. Data showed that 68.87% of the farmers did not agree with insurance. The results of the ordinal logit model at five different levels indicated that awareness of insurance benefits, insurance record, previous-year yield, educational level, the standard deviation of income, orchard area, and satisfaction with insurance services are the main variables influencing the demand for insurance. The coefficient of variation of the likelihood of insurance adoption was estimated at all five levels. A 1% increase in satisfaction with insurance services increases the likelihood of a person’s shift from the ‘strongly disagree’ category to the ‘disagree’ category by 6.3%. Older date farmers abstain from insurance adoption, which needs reflection given the religious view in the region on interest rates on the one hand and the resistance of older people against modern risk management methods on the other.
Socio-Demographic Characteristics and Outcomes of Pregnant Women Who Delivered Before and After Ending the One-Child Policy in China: A Comparative Study
Authors: Zhang Xiaohui , Lou Haifeng , Tang Xuejuan , Chen Xiaoli 
Published: January 2021

Abstract
Background: Recently, Chinese new fertility policy has received widespread public attention. However, there are limited studies on the comprehensive epidemiology of maternal and infant health with respect to the characteristic changes of childbearing women. In this study, we compared the changes in socio-demographic characteristics and pregnancy outcomes of childbearing women before and after ending the one-child policy in Zhejiang province of China.Methods: This was a multicenter and cross-sectional study. Data were from three representative hospitals in Zhejiang province by stratified random sampling. Women who gave birth in November 2012-2014-2016 were recruited, according to the time of the change in fertility policy. We explored the differences in maternal sociodemographic characteristics and pregnancy outcomes.Results: Totally, 11,718 women were recruited, including 3480-4044-4194 in November 2012-2014-2016 respectively. Results showed the proportion of women with advanced age, relative higher education, higher number of gravidity and parity, previous cesarean section and births in a provincial hospital increased over time. Of multipara, they were more likely to be over ≥ 35 years old, to give birth vaginally and to have pregnancy complications and multiple birth, particularly in 2016 than in 2012. Of those primipara, the proportion of women with advanced age remained stable during periods. Further, both the percentage of vaginal delivery and instrumental vaginal delivery were higher in 2014 and 2016 than in 2012. The same period, the proportions of pregnancy complications, and multiple births significantly rose, but stillbirth and birth decreased in 2014 than in 2012 among women who never delivered.Conclusions: Some adverse pregnancy outcomes increased as some key characteristics of childbearing women changed after ending the one-child policy. It suggested that policy provider and medical staff to strengthen consistent healthcare regarding to changes in birth policy.