bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tripartite motif 10 regulates cardiac hypertrophy by targeting the PTEN/AKT pathway
Authors: Yang Hui , Wang Xiao‐Xiao , Zhou Chun‐Yu , Xiao Xue , Tian Cui , Li Hui‐Hua , Yin Chun‐Lin , Wang Hong‐Xia 
Published: April 2020

AbstractThe pathogenesis of cardiac hypertrophy is tightly associated with activation of intracellular hypertrophic signalling pathways, which leads to the synthesis of various proteins. Tripartite motif 10 (TRIM10) is an E3 ligase with important functions in protein quality control. However, its role in cardiac hypertrophy was unclear. In this study, neonatal rat cardiomyocytes (NRCMs) and TRIM10‐knockout mice were subjected to phenylephrine (PE) stimulation or transverse aortic constriction (TAC) to induce cardiac hypertrophy in vitro and in vivo, respectively. Trim10 expression was significantly increased in hypertrophied murine hearts and PE‐stimulated NRCMs. Knockdown of TRIM10 in NRCMs alleviated PE‐induced changes in the size of cardiomyocytes and hypertrophy gene expression, whereas TRIM10 overexpression aggravated these changes. These results were further verified in TRIM10‐knockout mice. Mechanistically, we found that TRIM10 knockout or knockdown decreased AKT phosphorylation. Furthermore, we found that TRIM10 knockout or knockdown increased ubiquitination of phosphatase and tensin homolog (PTEN), which negatively regulated AKT activation. The results of this study reveal the involvement of TRIM10 in pathological cardiac hypertrophy, which may occur by prompting of PTEN ubiquitination and subsequent activation of AKT signalling. Therefore, TRIM10 may be a promising target for treatment of cardiac hypertrophy.
Effect of Ataluren on dystrophin mutations
Authors: Berger Joachim , Li Mei , Berger Silke , Meilak Michelle , Rientjes Jeanette , Currie Peter D. 
Published: April 2020

AbstractDuchenne muscular dystrophy is a severe muscle wasting disease caused by mutations in the dystrophin gene (dmd). Ataluren has been approved by the European Medicines Agency for treatment of Duchenne muscular dystrophy. Ataluren has been reported to promote ribosomal read‐through of premature stop codons, leading to restoration of full‐length dystrophin protein. However, the mechanism of Ataluren action has not been fully described. To evaluate the efficacy of Ataluren on all three premature stop codons featuring different termination strengths (UAA > UAG > UGA), novel dystrophin‐deficient zebrafish were generated. Pathological assessment of the muscle by birefringence quantification, a tool to directly measure muscle integrity, did not reveal a significant effect of Ataluren on any of the analysed dystrophin‐deficient mutants at 3 days after fertilization. Functional analysis of the musculature at 6 days after fertilization by direct measurement of the generated force revealed a significant improvement by Ataluren only for the UAA‐carrying mutant dmdta222a. Interestingly however, all other analysed dystrophin‐deficient mutants were not affected by Ataluren, including the dmdpc3 and dmdpc2 mutants that harbour weaker premature stop codons UAG and UGA, respectively. These in vivo results contradict reported in vitro data on Ataluren efficacy, suggesting that Ataluren might not promote read‐through of premature stop codons. In addition, Ataluren had no effect on dystrophin transcript levels, but mild adverse effects on wild‐type larvae were identified. Further assessment of N‐terminally truncated dystrophin opened the possibility of Ataluren promoting alternative translation codons within dystrophin, thereby potentially shifting the patient cohort applicable for Ataluren.
Release rate determination from in situ gel forming PLGA implant: a novel ‘shape-controlled basket in tube’ method
Authors: Zhang Qiangnan , Fassihi Reza 
Published: April 2020

Abstract

Objectives
This study aimed to examine the impact of syringe-needle assembly differences in making implants of different shapes as well as its influence on the release kinetics and investigate the release kinetics of the in situ forming implant under various release arrangements.

Methods
PLGA in situ forming implant was prepared in different shape and then subjected to in vitro release testing. Mathematical modelling was used to investigate drug release mechanisms.

Key findings
The in situ forming implant was investigated for the first time how implant shapes can affect release results. It was demonstrated that implant shape differences could lead to significant variation in the release data. Here, we addressed this issue by developing a shape-controlled method to provide a consistent surface to volume ratio and, therefore, a reliable release result. Injectability in the in vitro release was discussed for the first time. Comparisons between various release methods were also evaluated. The release arrangement was found to be of great importance in release kinetics.

Conclusions
The developed ‘shape-controlled basket in tube’ method can provide the most reproducible release profiles by minimizing implant adhesion to the release vessels or movement without sacrificing full contact between the release medium and the implant surface.
NIETZSCHE AND UNAMUNO ON CONATUS AND THE AGAPEIC WAY OF LIFE
Authors: Oya Alberto 
Published: April 2020

AbstractUnamuno saw in his defense of religious faith a response to Nietzsche’s criticisms of the Christian, agapeic way of life. To Nietzsche’s claim that engaging in this way of life is something antinatural and life‐denying, insofar as it goes against the (alleged) natural tendency to increase one’s own power, Unamuno responded that an agapeic way of life is precisely a direct expression of this natural tendency. Far from being something that goes against our natural inclinations, Unamuno says, an agapeic way of life is a life‐affirming exercise, something we are led to given our own natural condition. Hence, the aim of this essay is to comment on Unamuno’s criticism of Nietzsche and to point out the philosophical relevance of Unamuno’s attempt to provide a natural foundation for religious faith when assessing Nietzsche’s criticisms of the possibility of carrying out a Christian, agapeic way of life.
Ancient GreekPhilosophiain India as a Way of Life
Authors: Moore Christopher 
Published: April 2020

AbstractThe Greek identification of certain Indian people asphilosophoiat the end of the fourth centurybceprovides unique information about the meaning of the termphilosophia, especially with respect to its reference to a certain kind of “way of life” (bios), at the time of its greatest maturity (at the start of the Hellenistic period). TheIndicaof Megasthenes, an ambassador to northern India after the death of Alexander, is our most important evidence; fragments from earlier works by Nearchus and Onesicritus, intellectuals in Alexander’s cortege, provide corroboration. This essay argues that late classical Greeks did largely think ofphilosophiaas a way of life.
PHILOSOPHY AS A WAY OF LIFE TODAY
Authors: Faustino Marta 
Published: April 2020

AbstractThis essay discusses the possibility, relevance, and pertinence of a reactivation of philosophy as a way of life today on the basis of Pierre Hadot’s account and recent scholarly approaches to the topic. In the wake of John Sellars, it regards philosophy as a way of life as a metaphilosophical option that can still be applied today. The essay starts by addressing John Cooper’s criticism of philosophy as a way of life in the contemporary philosophical landscape and shows that, against the background of the model defined by Hadot, Cooper’s objections do not apply. It then illustrates the clear expression of the model in contemporary philosophy through the particular case of Nietzsche. It concludes by outlining some of the considerations that, in the wake of Hadot, Sellars, Michael Chase, and others, might persuade researchers, teachers, and academics to embrace philosophy as a way of life in their work and lives.
Self‐prediction in practical reasoning: Its role and limits
Authors: White Stephen J. 
Published: April 2020

AbstractAre predictions about how one will freely and intentionally behave in the future ever relevant to how one ought to behave? There is good reason to think they are. As imperfect agents, we have responsibilities of self‐management, which seem to require that we take account of the predictable ways we're liable to go wrong. I defend this conclusion against certain objections to the effect that incorporating predictions concerning one's voluntary conduct into one's practical reasoning amounts to evading responsibility for that conduct. There is, however, some truth to this sort of objection. To understand the legitimate role of self‐prediction in practical reasoning, we need to distinguish instances of coping responsibly with an anticipated failure to behave as one ought, on the one hand, from mere acquiescence in one's flaws, on the other. I argue that, to draw this distinction, we must recognize certain limits on the use of self‐prediction as a ground of choice.
Evolution of NLR resistance genes with noncanonical N‐terminal domains in wild tomato species
Authors: Seong Kyungyong , Seo Eunyoung , Witek Kamil , Li Meng , Staskawicz Brian 
Published: April 2020

Summary

Nucleotide‐binding and leucine‐rich repeat immune receptors (NLRs) provide resistance against diverse pathogens. To create comparative NLR resources, we conducted resistance gene enrichment sequencing (RenSeq) with single‐molecule real‐time sequencing of PacBio for 18 accessions in Solanaceae, including 15 accessions of five wild tomato species.

We investigated the evolution of a class of NLRs, CNLs with extended N‐terminal sequences previously named Solanaceae Domain. Through comparative genomic analysis, we revealed that the extended CNLs (exCNLs) anciently emerged in the most recent common ancestor between Asterids and Amaranthaceae, far predating the Solanaceae family.

In tomatoes, the exCNLs display exceptional modes of evolution in a clade‐specific manner. In the clade G3, exCNLs have substantially elongated their N‐termini through tandem duplications of exon segments. In the clade G1, exCNLs have evolved through recent proliferation and sequence diversification. In the clade G6, an ancestral exCNL has lost its N‐terminal domains in the course of evolution.

Our study provides high‐quality NLR gene models for close relatives of domesticated tomatoes that can serve as a useful resource for breeding and molecular engineering for disease resistance. Our findings regarding the exCNLs offer unique backgrounds and insights for future functional studies of the NLRs.
Prosthodontic rehabilitation of head and neck cancer patients—Challenges and new developments
Authors: Vosselman Nathalie , Alberga Jamie , Witjes Max H. J. , Raghoebar Gerry M. , Reintsema Harry , Vissink Arjan , Korfage Anke 
Published: April 2020

AbstractHead and neck cancer treatment can severely alter oral function and aesthetics, and reduce quality of life. The role of maxillofacial prosthodontists in multidisciplinary treatment of head and neck cancer patients is essential when it comes to oral rehabilitation and its planning. This role should preferably start on the day of first intake. Maxillofacial prosthodontists should be involved in the care pathway to shape and outline the prosthetic and dental rehabilitation in line with the reconstructive surgical options. With the progress of three‐dimensional technology, the pretreatment insight in overall prognosis and possibilities of surgical and/or prosthetic rehabilitation has tremendously increased. This increased insight has helped to improve quality of cancer care. This expert review addresses the involvement of maxillofacial prosthodontists in treatment planning, highlighting prosthodontic rehabilitation of head and neck cancer patients from start to finish.
Chromosome doubling of androgenic haploid plantlets of rice (Oryza sativa) using antimitotic compounds
Authors: Hooghvorst Isidre , Ribas Pol , Nogués Salvador 
Published: April 2020

AbstractThe regeneration of haploid plantlets is considered as a bottleneck in rice anther culture. In this study, an antimitotic chromosome doubling method, simple and efficient, of androgenic haploid plantlets resulted in an efficient doubled haploid obtainment. Through chromosome doubling capacity comparison of the three antimitotic compounds (colchicine, trifluralin and oryzalin), colchicine at 500 and 625 mg/L without supplementing with DMSO was found to be the best antimitotic treatment, with a chromosome doubling capacity of 40%. Furthermore, the in vitro growth of plantlets was followed to analyse the effects of antimitotic compounds. Colchicine treatments were more toxic than dinitroanilines, and colchicine DMSO‐supplemented treatments had significant lower values on shoot growth. On the other hand, dinitroaniline compounds impeded root growth, provoked helical growth of shoot and caused the apparition of white nodules in the base of the plantlet due to sprouting abortion. In this study, a protocol for doubled haploid plant recovery was established taking advantage from androgenic haploid plantlets in order to increase the number of doubled haploid plantlets produced after an anther culture protocol.
Establishing a nurse‐managed health center for the urban poor in Dhaka, Bangladesh
Authors: Lee Tae Wha , Lee Su Jeong , Kim Myeong Seon , Choi Yoona 
Published: April 2020

AbstractObjectivesDespite the rise in community health problems in populations living in urban slums, activities of community health nurses are limited in Bangladesh. This study aimed to describe how a nurse‐managed health center (NMHC) was developed in Dhaka, Bangladesh.DesignAn exploratory‐descriptive research design was used.Sample and MeasurementsThe commitment phase was conducted to establish partnerships in the community. Involvement of community leaders and members and partnerships with various organizations were established successfully in the commitment phase. The assessment phase was completed by implementing personal interviews, community site visits, and household surveys of 172 households in the community. Action plans were developed and strategies were followed to change the community during the planning phase.ResultsHousehold survey results showed that community people suffered from non‐communicable diseases, risk behaviors, and inadequate housing conditions. The high‐priority community needs included nutrition and chronic management services, behavior change programs, and a clean environment. Action plans for health programs based on community needs and strategies such as securing manpower and equipment were developed.ConclusionsThe systematic process of creating a NMHC, and the necessity of the community health nurse's role in responding to health needs of the urban poor in Dhaka, Bangladesh was confirmed.
Health is not all about salads: An ethnographic study on health perceptions and health behavior among socially marginalized Danish men
Authors: Pedersen Annette , Haslund‐Thomsen Helle , Curtis Tine , Grønkjær Mette 
Published: April 2020

AbstractBackgroundThe effects of social marginalization and health are well‐documented, revealing a complex association between health perceptions and health behavior. This is especially evident among socially marginalized men.ObjectiveThis study aims to explore health perceptions and health behaviors influencing overall health among socially marginalized men who seem to not benefit from existing municipal health care in a large Danish municipality. These men have varying health challenges and engage in risky health behaviors, such as alcohol drinking, tobacco smoking, and lack of physical activity.Design and sampleThis was an ethnographic study including 200 hr of participant observations and 25 interviews with socially marginalized men aged 45–65.ResultsHealth was perceived as related to the ability to participate in daily life activities, such as getting around effortlessly and the ability to work. Alcohol drinking appeared to be part of a complex approach in managing everyday life, including various personal physical and mental health challenges and admission to social networks.ConclusionOverall, the results provide important insight into how health perceptions and health behaviors are embedded in the men's everyday lives. This knowledge may be used by public health nurses and other health professionals in providing and developing health services.
Orthodontic treatment in a patient with cherubism: Benefits and limitations
Authors: Miranda Galvis Marisol , Faustino Isabel Schausltz Pereira , Ferraz Francisco Carlos , Castelli Sanchez Flavio José , Santos‐Silva Alan Roger , Lopes Marcio Ajudarte 
Published: April 2020

AbstractAimCherubism is an uncommon hereditary disease that leads to the development of giant cell lesions in the jaws, alterations in the dentition, and malocclusion. The biological behavior of bones to orthodontic forces in these patients is not described in the literature, leading dentists to avoid this management. The aim of this article was to describe a case report of management with orthodontics. We present details regarding clinicoradiographic features, diagnosis, treatment, and follow‐up.Case reportA 12‐year‐old male patient diagnosed with cherubism presented to our service with complaints about his esthetic facial and dental appearance. Management was interdisciplinary, including careful and controlled orthodontic treatment. The results were satisfactory; alignment, dental leveling, and correction of the malocclusion were achieved.ConclusionPatients with cherubism may benefit from orthodontics, improving oral function, and esthetic and psychosocial well‐being. The orthodontic treatment might be performed according to the severity of clinical manifestation, expectations of the patients, and limitations of each case.
Roles of BdUNICULME4 and BdLAXATUM‐A in the non‐domesticated grass Brachypodium distachyon
Authors: Magne Kévin , Liu Shengbin , Massot Sophie , Dalmais Marion , Morin Halima , Sibout Richard , Bendahmane Abdelhafid , Ratet Pascal 
Published: April 2020

SUMMARYIn cultivated grasses, tillering, spike architecture and seed shattering represent major agronomical traits. In barley, maize and rice, the NOOT‐BOP‐COCH‐LIKE (NBCL) genes play important roles in development, especially in ligule development, tillering and flower identity. However, compared with dicots, the role of grass NBCL genes is underinvestigated. To better understand the role of grass NBCLs and to overcome any effects of domestication that might conceal their original functions, we studied TILLING nbcl mutants in the non‐domesticated grass Brachypodium distachyon. In B. distachyon, the NBCL genes BdUNICULME4 (CUL4) and BdLAXATUM‐A (LAXA) are orthologous, respectively, to the barley HvUniculme4 and HvLaxatum‐a, to the maize Zmtassels replace upper ears1 and Zmtassels replace upper ears2 and to the rice OsBLADE‐ON‐PETIOLE1 and OsBLADE‐ON‐PETIOLE2/3. In B. distachyon, our reverse genetics study shows that CUL4 is not essential for the establishment of the blade–sheath boundary but is necessary for the development of the ligule and auricles. We report that CUL4 also exerts a positive role in tillering and a negative role in spikelet meristem activity. On the other hand, we demonstrate that LAXA plays a negative role in tillering, positively participates in spikelet development and contributes to the control of floral organ number and identity. In this work, we functionally characterized two new NBCL genes in a context of non‐domesticated grass and highlighted original roles for grass NBCL genes that are related to important agronomical traits.
Single‐incision percutaneous drilling technique to achieve hemiepiphysiodesis of the distal metacarpus in foals with metacarpophalangeal varus deformities
Authors: Dubois Benjamin B. , Rodgerson Dwayne H. 
Published: April 2020

AbstractObjectiveTo describe a drilling technique for hemiepiphysiodesis of the distal lateral metacarpal physis and report the outcome of treated foals.Study designRetrospective case series.Sample populationEleven thoroughbred foals.MethodsWhile horses were under general anesthesia, the lateral aspect of the distal metacarpal physis was approached through a single small incision by using a power drill. The drill bit was placed at the level of the physis under radiographic guidance. A 4.5‐mm drill bit was passed several times through the lateral growth plate to remove the cartilage in a fan‐like pattern. Postoperative outcomes consisted of clinical assessment and farm manager/owner satisfaction. Cosmetics and radiographic appearance of the surgical site were assessed when the horses were yearlings.ResultsThe procedure was performed in 16 limbs of 11 thoroughbred foals with a median age of 113.5 days (range, 90‐129). Median age at postoperative follow‐up was 422 days (range, 366 to 452). The procedure stopped the progression of a metacarpophalangeal varus deformity in all the limbs treated, determined by visual clinical evaluation and farm manager's satisfaction with subjectively excellent radiographic images and cosmetic outcomes at yearling age.ConclusionPhysis ablation was consistently achieved in these 11 foals with developing growth deformities of the distal metacarpus.Clinical significanceThis drilling technique may offer a minimally invasive, safe, and simple technique to manage distal limb conformation in foals without placement of implants. Additional quantitative data are required to assess its effectiveness relative to other options.
Evaluation of L7‐S1 nerve root pathology with low‐field MRI in dogs with lumbosacral foraminal stenosis
Authors: Lichtenhahn Vera , Richter Henning , Gödde Thomas , Kircher Patrick 
Published: April 2020

AbstractObjectiveTo describe low‐field MRI findings associated with lumbosacral foraminal stenosis and radiculopathy and correlate these with clinical signs.Study designRetrospective study.AnimalsClient‐owned dogs (n = 240) that underwent a clinical examination and standardized MRI protocol of the lumbosacral junction.MethodsMedical records of dogs with degenerative lumbosacral stenosis with neurological clinical evaluation and MRI of the lumbosacral junction were used to describe imaging pathologies and relate them to clinical status.ResultsIn total, 480 L7 neuroforamina were evaluated. A loss of foraminal fat signal was identified in 364 of 480 neuroforamina of which 87.9% (n=320) showed also concurrent nerve root changes. Magnetic resonance imaging features of L7 radiculopathy included nerve root enlargement and hyperintensity to surrounding connective tissue in dorsal oblique gradient echo short time inversion recovery sequences and specific changes in shape, size, or position of the nerve root in transverse T1‐weighted sequences. Radiculopathy was noted as a consequence of either circumferential (entrapment) or focal (impingement) foraminal stenosis. Lateral vertebral spondylotic and intervertebral facet joint changes were the most common underlying spinal and neuroforaminal pathologies. Clinical signs were present in the ipsilateral hind leg in 85% (n = 65) of dogs with unilateral lumbosacral imaging findings.ConclusionA loss of foraminal fat signal was likely to be associated with L7 radiculopathy and foraminal stenosis. Unilateral lesions were generally associated with clinical signs on the ipsilateral limb.Clinical significanceLoss of foraminal fat signal revealed by low‐field MRI should prompt the assessment of concurrent radiculopathy and underlying stenosis, and in coherence with clinical findings, when is combined with clinical findings, improves the diagnosis of lumbosacral foraminal stenosis.