bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide analysis of Jatropha curcas MADS-box gene family and functional characterization of the JcMADS40 gene in transgenic rice
Authors: Tang Yuehui , Wang Jian , Bao Xinxin , Wu Qian , Yang Tongwen , Li Han , Wang Wenxia , Zhang Yizhen , Bai Nannan , Guan Yaxin , Dai Jiaxi , Xie Yanjie , Li Shen , Huo Rui , Cheng Wei 
Published: April 2020

Abstract
Background: Physic nut (Jatropha curcas), a non-edible oilseed plant, is among the most promising alternative energy sources because of its high seed oil content, rapid growth and extensive adaptability. Proteins encoded by MADS-box genes are important transcription factors involved in the regulation of plant growth, seed development and responses to abiotic stress. However, there has been no in-depth research on the MADS-box genes and their roles in physic nut. Results: In the present study, 63 MADS-box genes (JcMADSs) were identified in the physic nut genome, and classed into five groups (MIKCC, Mα, Mβ, Mγ, MIKC*) based on phylogenetic comparison with Arabidopsis homologs. Expression profile analysis based on RNA-seq suggested that many JcMADS genes were expressed most strongly in seeds, and seven of them responded in leaves to at least one abiotic stressor (drought and/or salinity) at one or more time points. Transient expression analysis and a transactivation assay indicated that JcMADS40 is a nucleus-localized transcriptional activator. Plants overexpressing JcMADS40 did not show altered plant growth, but the overexpressing plants did exhibit reductions in grain size, grain length, grain width, 1000-seed weight and yield per plant. Further data on the reduced grain size in JcMADS40-overexpressing plants supported the putative role of JcMADS genes in seed development. Conclusions: This study will be useful in understanding the involvement of MADS-box genes in growth and development in addition to their functions in abiotic stress resistance, and will ultimately form the basis for functional characterization and the exploitation of candidate genes for the genetic engineering of physic nut.
Colorectal carcinomas with mucinous differentiation are associated with high frequent mutation of KRAS or BRAF mutations, irrespective of quantity of mucinous component.
Authors: Li Xiaodong , Sun Katherine , Liao Xiaoyan , Gao Haijuan , Zhu Hongfa , Xu Ruliang 
Published: April 2020

Abstract
Background: Mucinous adenocarcinoma (MAC) is a distinct type of colorectal cancer (CRC) associated with poor response to treatment and poorer prognosis. MAC is diagnosed by WHO definition when the extracellular mucin is more than 50% of the lesion. We aimed at assessing the gene expression profiles of the CRCs with any mucinous features (>5%) in a retrospective study. Methods: The data of a 50-gene next generation sequencing (NGS) panel of 166 CRCs was analyzed and the gene mutational profile with morphologic features was correlated. Results: We identified the different genetic mutation profiles between CRCs with and without mucinous component, but noticed a similar genetic profile between MACs and CRCs with mucinous component, irrespective of the percentage (if mucinous component more than 5%). The different genetic mutation profile related to MSI status was also identified between two groups of tumors. The most frequent mutations in CRCs with mucinous component are KRAS (28/49, 57.1%) and BRAF (19/49, 38.7%), PIK3CA (16/49, 32.6%), followed by APC (12/49, 24.5%) and TP53 (11/49, 22.5%). The combined mutation frequency of the two key factors in the EGFR signaling pathway, KRAS and BRAF, in the CRCs with and without mucinous component is 95.9% and 52.1%, respectively. Conclusions: The dysregulation of EGFR pathway plays a critical role in the development of CRCs with mucinous component, irrespective of the percentage. The result suggested that the current cut off of 50% mucin component to define mucinous adenocarcinoma might be challengeable.
Significance of incident reports by medical doctors for organizational transparency and driving forces for patient safety
Authors: Fukami Tatsuya , Uemura Masakazu , Nagao Yoshimasa 
Published: April 2020

Abstract
Background Incident reporting is an effective strategy used to enhance patient safety and quality improvement in healthcare. An incident is an event that could eventually result in harm to a patient. The aim of this study is to re-evaluate the importance of reporting by medical doctors to improve quality in healthcare and patient safety. Methods We conducted a retrospective analysis of the reported incidents registered in our institutional database from April 1 st 2015 to March 31 st 2019, classified according to eight variables proposed by the National University Hospital Council of Japan, to determine the type of incidents and their potential harm to patients. Results Registered reports totalled 43,775, approximately 8% of which arise annually from medical doctors in clinical departments. Incidents with higher impact on patients have significantly increased the rate of reporting by medical doctors. The most frequent types of report overall concerned medication incidents, followed by infusion lines, drainage-tube devices, cure, examination, and treatment outside the operating room. The most frequent reports by medical doctors involved operation-related incidents, followed by cure, examination, treatment outside the operation room, and medications. Conclusion Reporting by medical doctors reflects the organizational transparency and the driving forces behind patient safety and quality improvement in healthcare. Efforts toward seamless improvement in patient safety and quality at our hospital continue apace.
Impact of fecal microbiota transplantation on chronic recurrent pouchitis in ulcerative colitis with ileo-anal anastomosis: study protocol for a prospective, multicenter, double-blind, randomized, controlled trial
Authors: Trang-Poisson Caroline , Kerdreux Elise , Poinas Alexandra , Planche Lucie , Sokol Harry , Bemer Pascale , Cabanas Karine , Hivernaud Eliane , Biron Laetitia , Flet Laurent , Montassier Emmanuel , Garcasson Ghislaine Le , Chiffoleau Anne , Jobert Alexandra , Lepelletier Didier , Caillon Jocelyne , Pape Patrice Le , Imbert Berthe-Marie , Bourreille Arnaud 
Published: April 2020

Abstract
Background: Almost 15 % of patients with ulcerative colitis (UC) will require a proctocolectomy with ileal pouch–anal anastomosis (IPAA) as a result of fulminant colitis, dysplasia, cancer, or medical refractory diseases. Around 50% will experience pouchitis, an idiopathic inflammatory condition involving the ileal reservoir, responsible for digestive symptoms, deterioration in quality of life, and disability. Though the majority of initial cases of pouchitis are easily managed with a short course of antibiotics, in about 10% of cases, inflammation of the pouch becomes chronic with very few treatments available.Previous studies have suggested that manipulating the composition of intestinal flora through antibiotics, probiotics, and prebiotics achieved significant results for treating acute episodes of UC-associated pouchitis. However, currently there is no established effective treatment for chronic antibiotic-dependent pouchitis. Fecal microbiota transplantation (FMT) is a novel therapy involving the transfer of normal intestinal flora from a healthy donor to a patient with a medical condition potentially caused by the disrupted homeostasis of intestinal microbiota or dysbiosis.Methods Our project aims to compare the delay of relapse of chronic recurrent pouchitis after FMT versus sham transplantation. Forty-two patients with active recurrent pouchitis after having undergone an IPAA for UC will be enrolled at 12 French centers. The patients who respond to antibiotherapy will be randomized at a ratio of 1:1 to receive either FMT or sham transplantation. Discussion: On April 30, 2014, WHO published an alarming report on antibiotic resistance. Finding an alternative medical treatment to antibiotics in order to prevent relapses of pouchitis is therefore becoming increasingly important given the risk posed by multiresistant bacteria. Moreover, if the results of this study are conclusive, FMT, which is less expensive than biologics, could become a routine treatment in the future.Trial registration: The trial, published on May 14, 2018, is registered at ClinicalTrials.Gov under registration number NCT03524352: https://clinicaltrials.gov/ct2/show/NCT03524352?term=NCT03524352&rank=1
The role of reducing incision complications of surgical techniques used in groin lymphadenectomy for Vulval Malignancies
Authors: Zhang Xiao Ling , Sheng xiugui , Liu Naifu , Chen Jinlong , Zhang Tingting , Gao Fufeng 
Published: April 2020

Abstract
Background Inguinal lymphadenectomy plays an important role in the cure of patients with inguinal metastasis from vulval cancer or prophylactic inguinal lymphadenectomy.Unfortunately, this treatment is often associated with multiple postoperative complications. This work set out to investigate the efficacy and tolerance of negative pressure without subcuticular suture versus routine manipulation after inguinal lymphadenectomy for patients with vulval malignancies Methods 75 patients with vulval malignancies were allocated into two groups depending on their clinical stages, with one of them (38 patients included, A group) being subjected to negative pressure drainage without subcuticular suture and the other (37 patients included, B group) to routine drainage with subcuticular suture after treated with inguinal lymphadenectomy. The operative time, seroma, hematoma, hemorrhage, wound infection, skin flap necrosis, were selected as the monitored parameters, through which the above two groups were compared with each other using t test, c 2 analysis. Results (1) The median operative time for unilateral inguinal lymphadenectomy was 49 min(33min～70 min ) in A group, compared to 63 min(50～85 min) in B group(P<0.01), and it was 3 min(2.5min～5min ) 12.5 min(7～15 min) for only incision closing time(P＜0.01). It was 11.7d of the flap healing time in A group and 20.9d in B group. Further, the time(d) of antibiotics usage was still different, 3d and 5.5d. (2) Short-term lower extremity lymphedema occurred with 18 patients (31.6%) in the A group and 24 patients (44.4%) in the B group (P>0.05). Still, flap necrosis was observed with 26 patients (10.5%) in A group while 11developed (20.4%) in B group (P<0.05), and the incision infection was observed in 4 (7%)patient in A group instead of 16(29.6%) in B group (P＜0.01). However, there was no statistical difference in postoperative fever, acute cellulites, seroma, or lymphocyst formation. (3) Discomfort occurrence rate decreased by about 50% in patients subjected to study group compared with those to control group, while there was no remarkable difference between two groups in the occurrence rates of drain block (P>0.05). Conclusion The application of negative drain technique during inguinal lymphadenectomy for patients with vulval malignancies could result in significant decrease in the occurrence of postoperative complications and good tolerance, and should be widely put into clinical practice.
Safety of bronchoscopy in patients with hematologic disorders
Authors: Uruga Hironori , Sato Toshitaka , Nishida Aya , Uchida Naoyuki , Tsuji Masanori , Moriguchi Shuhei , Takahashi Yui , Ogawa Kazumasa , Murase Kyoko , Hanada Shigeo , Takaya Hisashi , Miyamoto Atsushi , Morokawa Nasa , Kimura Muneyoshi , Araoka Hideki , Tsuchihashi Rumiko , Asano-Mori Yuki , Wake Atsushi , Taniguchi Shuichi , Kishi Kazuma 
Published: April 2020

Abstract
Background: To evaluate the safety of bronchoscopy and describe factors affecting its complication rate in patients with hematologic disorders. Methods: Between January 2009 and December 2018, 316 bronchoscopies in 282 patients with hematologic disorders and pulmonary lesions were performed at our institution. The bronchoscopic procedure used and its complications were evaluated. Results: The most common underlying disease was acute myeloid leukemia (134/282 patients, 47.5%). Platelet transfusion was performed the day before or the day of bronchoscopy in 42.4%, supplemental oxygen was administered before the procedure in 23.1%, and midazolam was used in 74.4%. Two patients (0.6%) were intubated within 48 hour of the procedure and prolonged oxygen desaturation (>48 hour) occurred in 3.8%. Multivariate analysis showed that only use of midazolam significantly reduced the risk of prolonged oxygen desaturation ( p = 0.01, hazard ratio 0.23, 95% confidence interval 0.07–0.75). Conclusions: Bronchoscopy is relatively safe in patients with hematologic disorders. Sedation with midazolam may reduce the risk of prolonged oxygen desaturation.
Pharmacological mechanism of Xiaoyao San in the treatment of polycystic ovary syndrome based on network pharmacology
Authors: Zhu Chunyu , Hu Yajun , Zheng Wangdong , Zhang Yanyan , Li Yiting , Li Junling , Wei Xiying 
Published: April 2020

Abstract
Background : Xiaoyao San(XYS) has been widely used in the treatment of polycystic ovary syndrome(PCOS), but its mechanism is not clear. The purpose of this study is to elucidate the mechanism of XYS in the treatment of PCOS from the aspects of active components, targets and pathways. The purpose of the study is to explore the molecular mechanism of XYS in the treatment of PCOS. Methods : TCMSP database, UniProt and Perl were used to screen and collect the active components and targets of XYS. The genes related to PCOS were searched in GeneCards database. Collect the related targets of PCOS and XYS, use STRING database and Cytoscape software to process the data visually and analyze topology, and screen the key components and targets in the network. The key targets were enriched by R Project to predict the mechanism of XYS in the treatment of PCOS. Results : 68 active components and 96 drug targets in XYS were screened out. 3648 PCOS related disease targets were collected. 66 targets of XYS for PCOS treatment were obtained after analysis. 21 key targets of NCOA2, PGR, PTGS1, PPARG and AR were constructed after topology analysis. 63 biological functions and 111 biological pathways were obtained after gene ontology(GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) Pathway enrichment analysis. Conclusions : XYS has the characteristics of multi-component, multi-target and multi-path. This study discussed the active components, targets and potential mechanism of XYS in the treatment of PCOS, which provided a new direction for further study of the mechanism of XYS in the treatment of PCOS, and provides more ideas for clinical treatment of PCOS.
Accreditation of schools as Health Promoting Schools and Health Profile Assessment of Selected Accredited Schools in a Northern City of India
Authors: Thakur JS , Sharma Meenakshi , Bharti Bhavneet , Kaur Rupinder , Sharma Meenakshi 
Published: April 2020

Abstract
Background: Accreditation is a public recognition of the achievement of required standards by an organization. It certifies the schools for their efforts in implementing health promotion initiatives following an assessment, further helping them in redeveloping and implementing effective HPS strategies.Methods: The current study was conducted in 206 schools of Chandigarh with the objective of assessment and classification on accreditation level (bronze, silver, gold and platinum levels) as health promoting schools; intervention for one year for further quality improvement and reassessment after one year from 2016-2019. Interventions in the form of capacity building, technical visits, supportive supervision to schools, sensitization of policymakers and key stakeholders, implementation of policy initiatives, use of social media, technical support and monitoring of activities) were provided to all schools. Comparative health profile assessment was undertaken 754 children from higher and 700 children from lower accredited schools by GSHS questionnaire.Results : Out of 206 schools, 203 participated in the baseline and 204 in endline assessment. The response rate was 99 %.Two schools which refused participation were excluded and not assessed. Schools (N=17) which participated in the 2011-2013 study were excluded from analysis. There was a statistically difference (p=0.01) in the improvement of accreditation level of the baseline and endline assessment (p<0.05). Overall, the proportion of schools at the gold level increased from 1(0.5%) in 2016 to 71(38%).Silver level from 9(5 %) to 57 (31%) of schools after intervention. The response rate in health profile assessment in higher and lower accredited schools was 95.9% and 92.7% respectively. The health profile higher accreditation level schools (N=754) were found better in hygiene practices protective factors (peer support at school, parental or guardian supervision), handling stress and less prone to injury as compared to lower accreditation level schools (N=700),(p<0.05).Conclusions: The schools accreditation was feasible and lead to significant improvement in accreditation level as compared to baseline assessment (p<0.05).The Health profile assessment was also found to be better in higher as compared to lower accredited schools.Therefore, the accreditation system was found to be feasible, effective and beneficial in terms of improving health profile of children in the school setting.
DNA fingerprinting of cultivated and wild genotypes of Curcuma species from agroclimatic regions of Chhattisgarh
Authors: Jain Akanksha , Jain Parag 
Published: April 2020

Abstract
In the present study we had tried to investigate different genotypes of Curcuma isolated from different agroclimatic zones of Chattisgarh based on molecular fingerprinting as they contain varying range of Curcumin. Genetic fingerprints of 25 genotypes of Curcuma caesia, Curcuma longa and Curcuma aromatica by using 13 and 11 SSR primers. These primers were used to determine the diversity in genes for their proper utilization, genotypic conservation and commercial production of curcuma genotypes. Among all the studied varieties, 13 ISSR and 11SSR primers generated 107 and 51 different amplified fragments, of which 97% and 86% showed polymorphism, respectively. Through all the ISSR and SSR primers in all the genotypes amplified 1080 and 567 loci out of which 102 polymorphic and 5 monomorphic alleles were found in ISSR primers. 47 polymorphic and 4 monomorphic alleles were found in SSR. Different ISSR and SSR markers were used to estimate the DNA fingerprinting, genetic similarity and distance among or between the Curcuma genotypes. UPGMA data scoring methods were to analyze the variations between these genotypes of Curcuma. Thus, this study provides a baseline data for conservation of these curcuma genotypes that could be an important research towards optimizing industry level production of Curcumin production.
Evaluation of SARS-CoV-2 existence in blood, urine, and rectal swab in positive patients with different virus titers
Authors: Mondanizadeh Mahdieh , Rahimi Ensiyeh , Sarmadian Hossein , Jamalian Mohammad , Khansarinejad Behzad 
Published: April 2020

Abstract
Understanding the spreading routes of SARS-CoV-2 is crucial for patient management and defining biosafety strategies for public and health care workers. In the current study, the virus shedding in upper respiratory as well as blood, stool, and urine specimens of infected patients was examined using reverse transcription real-time PCR assay (RT-qPCR).
Relationship of chest CT score with clinical characteristics of 108 patients hospitalized with COVID-19 in Wuhan, China
Authors: Zhang Jie , Meng Guangping , Li Wei , Shi Bingqing , Dong Hongna , Su Zhenzhong , Huang Qian , gao peng 
Published: April 2020

Abstract
Background: In December 2019, the outbreak of a disease subsequently termed COVID-19 occurred in Wuhan, China. The number of cases increased rapidly and spread to six continents. However, there is limited information on the chest computed tomography (CT) characteristics of affected patients. Objective: Analyze COVID-19 patients to determine the relationships of clinical characteristics, chest CT score, and levels of inflammatory mediators. Methods: This retrospective, single-center case series of 108 consecutive hospitalized patients with confirmed COVID-19 at Tongji Hospital, Tongji Medical College of HUST (Wuhan, China) was performed from January 1 to January 28, 2020. Patient demographics, comorbidities, clinical findings, chest CT results, and CT scores of affected lung parenchyma were recorded. The relationships between chest CT score with levels of systemic inflammatory mediators were determined.Results: All patients exhibited signs of significant systemic inflammation, including increased levels of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), procalcitonin, chest CT score, and a decreased lymphocyte (LY) count. Chest CT score had positive associations with white blood cell (WBC) count, CRP, ESR, procalcitonin, and abnormal coagulation function, and a negative association with LY count. Treatment with a glucocorticoid increased the LY count, reduced the CT score and CRP level, and improved coagulation function.Conclusions: COVID-19 infection is characterized by a systemic inflammatory response that affects the lungs, blood, digestive system, and circulatory systems. The chest CT score is a good indicator of the extent of systemic inflammation. Glucocorticoid treatment appears to reduce systemic inflammation in these patients.