bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of time interval between multidisciplinary team meeting and intended pancreatoduodenectomy on oncological outcomes
Authors:   Dutch Pancreatic Cancer Group, Steen M W , Rijssen L B , Festen S , Busch O R , Groot Koerkamp B , Geest L G , Hingh I H , Santvoort H C , Besselink M G , Gerhards M F 
Published: August 2020

Abstract

Background
Dutch guidelines indicate that treatment of pancreatic head and periampullary malignancies should be started within 3 weeks of the multidisciplinary team (MDT) meeting. This study aimed to assess the impact of time to surgery on oncological outcomes.

Methods
This was a retrospective population-based cohort study of patients with pancreatic head and periampullary malignancies included in the Netherlands Cancer Registry. Patients scheduled for pancreatoduodenectomy and who were discussed in an MDT meeting from May 2012 to December 2016 were eligible. Time to surgery was defined as days between the final preoperative MDT meeting and surgery, categorized in tertiles (short interval, 18 days or less; intermediate, 19–32 days; long, 33 days or more). Oncological outcomes included overall survival, resection rate and R0 resection rate.

Results
A total of 2027 patients were included, of whom 677, 665 and 685 had a short, intermediate and long time interval to surgery respectively. Median time to surgery was 25 (i.q.r. 14–36) days. Longer time to surgery was not associated with overall survival (hazard ratio 0·99, 95 per cent c.i. 0·87 to 1·13; P = 0·929), resection rate (relative risk (RR) 0·96, 95 per cent c.i. 0·91 to 1·01; P = 0·091) or R0 resection rate (RR 1·01, 0·94 to 1·09; P = 0·733). Patients with pancreatic ductal adenocarcinoma and a long time interval had a lower resection rate (RR 0·92, 0·85 to 0·99; P = 0·029).

Discussion
A longer time interval between the last MDT meeting and pancreatoduodenectomy did not decrease overall survival.
Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis
Authors: Huang Lu , Wan Yaqi , Dang Zhancui , Yang Peng , Yang Quanyu , Wu Shizheng 
Published: August 2020

AbstractObjectivesSequelae of stroke were mainly caused by neuronal injury. Oxygen is a key factor affecting the microenvironment of neural stem cells (NSCs), and oxygen levels are used to promote NSC neurogenesis. In this study, effects of intermittent hypoxic preconditioning (HPC) on neurogenesis were investigated in a rat model of middle cerebral artery occlusion (MCAO).MethodsSD rats were used to establish the MCAO model. Nissl staining and Golgi staining were used to confirm the neuronal injury status in the MCAO model. Immunofluorescence, transmission electron microscopy, Western blot, and qPCR were used to observe the effects of HPC on neurogenesis. At the same time, the hypothesis that HPC could affect proliferation, apoptosis, differentiation, and migration of NSC was verified in vitro.ResultsHypoxic preconditioning significantly ameliorated the neuronal injury induced by MCAO. Compared with MCAO group, the dendrites, Edu+/SOX2+, Edu+/DCX+, Edu+/NeuN+, Edu+/GFAP+, and Edu+/Tubulin+ positive cells in the HPC + MCAO group exhibited significantly difference. Similarly, axonal and other neuronal injuries in the HPC + MCAO group were also ameliorated. In the in vitro experiments, mild HPC significantly enhanced the viability of NSCs, promoted the migration of differentiated cells, and reduced apoptosis.ConclusionsOur results showed that HPC significantly promotes neurogenesis after MCAO and ameliorates neuronal injury.
Comparison of outcome in men and women after treatment in psychiatric security hospitals in Germany
Authors: Franke Irina , Rothe Philipp , Dudeck Manuela , Bezzel Adelheid , Klein Verena , Ormanns Norbert , Streb Judith 
Published: August 2020

Previous research has shown that forensic psychiatric treatment reduces reoffending, rehospitalization and premature mortality. Treatment outcome varies with diagnosis, but little is known about the influence of sex, psychosocial adjustment and aftercare.To assess these variables, we interviewed male and female patients discharged from three psychiatric security hospitals in Germany in the years 2010–2017. Participants were interviewed at discharge (n = 609) and 1 year later (n = 366) about reoffending, readmissions, substance use and psychosocial adjustment. Among patients with substance use disorder (SUD), 14% reoffended, 20% were re‐hospitalized and 60% maintained abstinence. Among patients with severe mental disorder, 5% reoffended and 13% were re‐hospitalized. Significant sex differences were found in offenders with SUD. The results suggest that sociodemographic and disorder‐related risk factors are associated with treatment success and that female patients with SUD might need a specific treatment approach. Sex‐specific aspects, diagnosis and psychosocial adjustment should be considered in forensic psychiatric treatment and risk assessment.
An immune‐related prognostic signature for predicting breast cancer recurrence
Authors: Tian Zelin , Tang Jianing , Liao Xing , Yang Qian , Wu Yumin , Wu Gaosong 
Published: August 2020

AbstractBreast cancer (BC) is the most common cancer among women worldwide and is the second leading cause of cancer‐related deaths in women. Increasing evidence has validated the vital role of the immune system in BC development and recurrence. In this study, we identified an immune‐related prognostic signature of BRCA that could help delineate risk scores of poor outcome for each patient. This prognostic signature comprised information on five danger genes—TSLP, BIRC5, S100B, MDK, and S100P—and three protect genes RARRES3, BLNK, and ACO1. Kaplan‐Meier survival curve showed that patients classified as low‐risk according to optimum cut‐off risk score had better prognosis than those identified within the high‐risk group. ROC analysis indicated that the identified prognostic signature had excellent diagnostic efficiency for predicting 3‐ and 5‐years relapse‐free survival (RFS). Multivariate Cox regression analysis proved that the prognostic signature is independent of other clinical parameters. Stratification analysis demonstrated that the prognostic signature can be used to predict the RFS of BC patients within the same clinical subgroup. We also developed a nomogram to predict the RFS of patients. The calibration plots exhibited outstanding performance. The validation sets (GSE21653, GSE20711, and GSE88770) were used to external validation. More convincingly, the real time RT‐PCR results of clinical samples demonstrated that danger genes were significantly upregulated in BC samples, whereas protect genes were downregulated. In conclusion, we developed and validated an immune‐related prognostic signature, which exhibited excellent diagnostic efficiency in predicting the recurrence of BC, and will help to make personalized treatment decisions for patients at different risk score.
Ionic Liquids with More than One Metal: Optical and Electrochemical Properties versus d‐Block Metal Combinations
Authors: Balischewski Christian , Behrens Karsten , Zehbe Kerstin , Günter Christina , Mies Stefan , Sperlich Eric , Kelling Alexandra , Taubert Andreas 
Published: August 2020

AbstractThirteen N‐butylpyridinium salts, including three monometallic [C4Py]2[MCl4], nine bimetallic [C4Py]2[M1−xaMxbCl4] and one trimetallic compound [C4Py]2[M1−y‐zaMybMzcCl4] (M=Co, Cu, Mn; x=0.25, 0.50 or 0.75 and y=z=0.33), were synthesized and their structure and thermal and electrochemical properties were studied. All compounds are ionic liquids (ILs) with melting points between 69 and 93 °C. X‐ray diffraction proves that all ILs are isostructural. The conductivity at room temperature is between 10−4 and 10−8 S cm−1. Some Cu‐based ILs reach conductivities of 10−2 S cm−1, which is, however, probably due to IL dec. This correlates with the optical bandgap measurements indicating the formation of large bandgap semiconductors. At elevated temperatures approaching the melting points, the conductivities reach up to 1.47×10−1 S cm−1 at 70 °C. The electrochemical stability windows of the ILs are between 2.5 and 3.0 V.
Two‐Dimensional NiIr@N‐Doped Carbon Nanocomposites Supported on Ni Foam for Electrocatalytic Overall Water Splitting
Authors: Xu You , Chai Xingjie , Liu Mengying , Ren Tianlun , Yu Shanshan , Wang Ziqiang , Li Xiaonian , Wang Liang , Wang Hongjing 
Published: August 2020

AbstractElectrochemical water splitting can provide a promising avenue for sustainable hydrogen production. Highly efficient electrocatalysts toward the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) are extremely important for the practical application of water splitting technology. Herein, a one‐step annealing strategy is reported for the fabrication of a metal–organic framework‐derived bifunctional self‐supported electrocatalyst, which is composed of two‐dimensional N‐doped carbon‐wrapped Ir‐doped Ni nanoparticle composites supported on Ni foam (NiIr@N‐C/NF). The resultant NiIr@N‐C/NF displays excellent electrocatalytic performance in 1.0 m KOH, with low overpotentials of 32 mV at 10 mA cm−2 for the HER and 329 mV at 50 mA cm−2 for the OER. Particularly, the HER‐OER bifunctional NiIr@N‐C/NF needs only 1.50 V to yield 10 mA cm−2 for overall water splitting.
Controlling the Number of Branches and Surface Facets of Pd‐Core Ru‐Branched Nanoparticles to Make Highly Active Oxygen Evolution Reaction Electrocatalysts
Authors: Myekhlai Munkhshur , Benedetti Tania M. , Gloag Lucy , Poerwoprajitno Agus R. , Cheong Soshan , Schuhmann Wolfgang , Gooding J. Justin , Tilley Richard D. 
Published: August 2020

AbstractProducing stable but active materials is one of the enduring challenges in electrocatalysis and other types of catalysis. Producing branched nanoparticles is one potential solution. Controlling the number of branches and branch size of faceted branched nanoparticles is one of the major synthetic challenges to achieve highly active and stable nanocatalysts. Herein, we use a cubic‐core hexagonal‐branch mechanism to synthesize branched Ru nanoparticles with control over the size and number of branches. This structural control is the key to achieving high exposure of active {10–11} facets and optimum number of Ru branches that enables improved catalytic activity for oxygen evolution reaction while maintaining high stability.
Organic Superbases in Recent Synthetic Methodology Research
Authors: Puleo Thomas R. , Sujansky Stephen J. , Wright Shawn E. , Bandar Jeffrey S. 
Published: August 2020

AbstractOrganic superbases are a distinct and increasingly utilized class of Brønsted base that possess properties complementary to common inorganic bases. This Concept article discusses recent applications of commercial organic superbases in modern synthetic methodologies. Examples of the advantages of organic superbases in three areas are highlighted, including the discovery of new base‐catalyzed reactions, the optimization of reactions that require stoichiometric Brønsted base, and in high‐throughput experimentation technology.
Preparation and Characterization of Porous Carbon from Mixed Leaves for High‐Performance Supercapacitors
Authors: Yang Xiaoxiang , Xu Jie , Chen Xin , Lei Yuli , Wang Lingling , Cheng Siyu , Li Yan , Lu Yuxuan , Zhu Yupeng , Chen Na 
Published: August 2020

Main observation and conclusionWith the increasing demand of sustainable and eco‐friendly resources, it is very attractive to use waste leaves for activated carbon (AC) preparation that can be used in supercapacitors. Considering the practically collected leaves are commonly in mixed nature, and extra efforts are needed to sort these mixtures, we here report the first preparation of AC using mixed leaves from different plants, including Platanus acerifolia (PA), Firmiana platanifolia (FP) and Pistia stratiotes (PS), and compared the results with single leaves‐derived AC materials. These leave‐derived AC samples were characterized with FESEM, EDS, FTIR and Raman spectroscopy, and electrochemical tests. The AC derived from mixed leaves (PA3FP1PS2AC) showed a good specific capacitance of 246 F·g−1 at 1 A·g−1 in aqueous 3 mol·L–1 KOH. This sample further showed the largest specific capacitance of 201 F·g−1 at 5 A·g−1, as compared with the non‐mixed AC samples, and a good stability of 100.0% capacitance retention over 1000 cycles. Utilizing mixed leaves for AC preparation is not only demonstrated to be a promising approach of low cost and high‐performance AC production for supercapacitor applications, but may also help environmental protection by reducing the invasive species problem.
Design and Application of a Rotatory Device for Detecting Transient Ca2+ Signals in Response to Mechanical Stimulation Using an Aequorin‐Based Ca2+ Imaging System
Authors: Peng Yingtong , Zheng Yu , Zhou Jinrun , Shang‐Guan Keke , Wang Huiquan , Liang Yan 
Published: August 2020

AbstractElevation of the cytosolic free calcium ion (Ca2+) concentration ([Ca2+]cyt) is one of the earliest responses to biotic and abiotic stress in plant cells. Among the various Ca2+ detection systems available, aequorin‐based luminescence Ca2+ imaging systems provide a relatively amenable and robust method that facilitates large‐scale genetic‐mutant screening based on [Ca2+]cyt responses. Compared to that mediated by chemical elicitors, mechanical stimulation–induced elevation of [Ca2+]cyt is considerably more rapid, occurring within 10 s following stimulation. Therefore, its assessment using aequorin‐based Ca2+ imaging systems represents a notable challenge, given that a time interval of ≥1 min is required to reduce the background light before operating the photon imaging detector. In this context, we designed a device that can rotate automatically within the confines of an enclosed dark box, and using this, we can record [Ca2+]cyt dynamics immediately after plants had been rotated to induce mechanical stimulation. This tool can facilitate the study of perception and early signal transduction in response to mechanical stimulation on a large scale based on [Ca2+]cyt responses. © 2020 Wiley Periodicals LLC.Basic Protocol 1: Detection of background luminance signals in aequorin‐transformed Arabidopsis seedlings using a photon imaging detectorBasic Protocol 2: Construction of the rotatory deviceBasic Protocol 3: Calcium measurement in Arabidopsis seedlings after rotatory stimulationBasic Protocol 4: Data analysis and processing
Monodisperse Clay Microballs for Tuning the Pseudogaps by Adsorption in Amorphous Photonic Structures
Authors: Okada Tomohiko , Hosoyamada Sho , Takada Chisato , Ohta Chiharu 
Published: August 2020

AbstractStructural color based on colloidal amorphous arrays has received growing interest in both natural and artificial systems; however few local structures on colloidal particles have been designed to tune the photonic pseudogap. We use an expandable interlayered inorganic nanosheet, a synthetic clay, on monodisperse colloidal silica spheres as constituents in an array. Interlayer expansion of the inorganic nanosheet by a cationic surfactant led to an increase in the size of the colloidal particles, resulting in a spectral redshift of the pseudogap. This phenomenon was related to the change in size of the monodisperse particles, which was induced by changing the defined layered local structure.
Monitoring CAR T‐cells using flow cytometry
Authors: Demaret Julie , Varlet Pauline , Trauet Jacques , Beauvais David , Grossemy Aurélien , Hégo Florent , Yakoub‐Agha Ibrahim , Labalette Myriam 
Published: August 2020

AbstractBackgroundChimeric antigen receptor (CAR) T‐cell therapy is considered as a major scientific breakthrough in cancer immunotherapy. The success of adoptive CAR T‐cell therapy for cancer has inspired researchers to expand indications into the area of solid tumors, autoimmune and infectious diseases. The most important factors influencing outcome and durability of the response after infusion of CAR T‐cell are proliferation and persistence of this cell subset. It becomes therefore important to detect easily and monitor circulating CAR T‐cells into blood samples. Approaches such as quantitative PCR (qPCR) or flow cytometry have been developed. The aim of this study was to set up and optimize a reachable flow cytometry technique using labeled CD19 protein for the measurement of CAR T‐cells in infusion bag and patient's blood.MethodsPatients receiving Yescarta in Cell Therapy Unit (Department of hematology, Lille university hospital, France) between April and October 2019 and healthy volunteers were included to set up the flow cytometry technique.Results and conclusionsWe assessed feasibility in clinic and suitability to routine workload of a flow cytometry technique to follow CAR T‐cells in infusion bag and patient's blood. With only a few manual steps, the present protocol allows the technician to perform this technique among other routine tasks, meaning a time to results of <2 hr after sample reception. We were also able to assess CAR T‐cell heterogenity in terms of CD4+ and CD8+ T lymphocytes within the subset. Moreover, this technique allows monitoring of both authority approved CD19 CAR T‐cell.
Fine needle aspiration cytology of cervical lymph nodes: Comparison of liquid based cytology (SurePath) and conventional preparation
Authors: Budhwar Archana , Kataria Sant Prakash , Kumar Sanjay , Singh Gajender , Kaushik Nidhi , Sen Rajeev 
Published: August 2020

AbstractBackgroundFine needle aspiration cytology (FNAC) is the first diagnostic step in patient with cervical lymphadenopathy because of its simplicity, safety and early availability of the results. Liquid‐based cytology (LBC) is an alternative processing method which is used for both gynecological and nongynecological samples. Literature reviewed show few studies comparing LBC with conventional preparation (CP).AimThe present study was undertaken to evaluate the efficacy of LBC and comparison of LBC and CP in cervical lymphadenopathy.Materials and MethodsIn this prospective study, a total of 75 cases of FNAC with cervical lymphadenopathy were included. The first pass was used for CP followed by LBC with the use of SurePath (SP) technique. Both the smears were compared for cellularity, background containing blood, cell debris, lymphoglandular bodies, stromal fragments, cytoarchitectural pattern, etc., by semiquantitative scoring system.ResultsThere was no statistical difference in the cellularity, cell architecture, and monolayer cells (P > .05). On the basis of background containing blood, cell debris, lympho‐glandular bodies, stromal fragments (P < .001), nuclear, and cytoplasmic details (P < .05), LBC was found to be superior to CP.ConclusionLBC is a relatively simple technique and superior to CP in respect of better nuclear and cytoplasmic details with loss of background blood and debris. It has a diagnostic accuracy equivalent to that of CP. However, use of both LBC and CP can result in better diagnostic accuracy.
Catheter‐based embolectomy prior to right ventricular mechanical circulatory support placement after heart transplantation
Authors: Jagadeesan Vikrant S. , Davidson Laura J. , Churyla Andrei , Benzuly Keith H. 
Published: August 2020

AbstractSevere acute isolated right ventricular failure has limited dedicated percutaneous temporary mechanical circulatory support options, especially after orthotopic heart transplantation. The advent of the Impella RP device provides a newer option, though an absolute contraindication to device placement is thrombus within the right heart. We present a novel case where catheter‐based embolectomy was used to evacuate right heart thrombus before Impella RP placement in a patient with severe acute right ventricular failure due to primary graft dysfunction after orthotopic heart transplantation.
Ordered Mesoporous Silica Incorporating Platinum Nanoparticles as Electrode Material for Paracetamol Detection
Authors: Hien Ngo Thi Thanh , Fort Ioana Carmen , Pham Thanh Huyen , Turdean Graziella Liana 
Published: August 2020

AbstractHigh ordered mesoporous materials (SBA‐15) modified with Al and/or B and Pt nanoparticles (Pt NPs) were used for preparing modified graphite paste electrodes (Pt/M−SBA‐15‐GPE, where M=Al−, B− or Al−B−) and applied for paracetamol (PA) detection. The electrodes were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Square wave voltammetry (SWV) technique was used to obtain the analytical parameters for PA detection. The acquired values of electrochemical and analytical parameters recommend the mesoporous compound containing Pt NPs to be used as composite electrode material for PA detection in real samples.
Electrospray: More than just an ionization source
Authors: Tycova Anna , Prikryl Jan , Kotzianova Adela , Datinska Vladimira , Velebny Vladimir , Foret Frantisek 
Published: August 2020

AbstractElectrospraying (ES) is a potential‐driven process of liquid atomization, which is employed in the field of analytical chemistry, particularly as an ionization technique for mass spectrometric analyses of biomolecules. In this review, we demonstrate the extraordinary versatility of the electrospray by overviewing the specifics and advanced applications of ES‐based processing of low molecular mass compounds, biomolecules, polymers, nanoparticles, and cells. Thus, under suitable experimental conditions, ES can be used as a powerful tool for highly controlled deposition of homogeneous films or various patterns, which may sometimes even be organized into 3D structures. We also emphasize its capacity to produce composite materials including encapsulation systems and polymeric fibers. Further, we present several other, less common ES‐based applications. This review provides an insight into the remarkable potential of ES, which can be very useful in the designing of innovative and unique strategies.
A comparison of patients with anorexia nervosa and women who are constitutionally thin
Authors: Bang Eun Byul , Ko Jeong Kyung , Kwag Kyung Hwa , Lee Gi Young , Kim Youl‐Ri 
Published: August 2020

AbstractObjectiveMany of the clinical features of anorexia nervosa (AN) can be secondary problems associated with malnutrition, which mask the primary features of AN. This study aimed to investigate the intrinsic features of AN by comparing AN patients to women who are constitutionally thin (CT).MethodTwenty‐six patients with AN and 53 CT women of similar body mass index (AN: 17.13 ± 1.73 kg/m2, CT: 16.62 ± 0.61 kg/m2) participated in the study. We examined medical findings, psychiatric features, and disturbed body image between the two groups.ResultsPatients with AN had earlier menarche but less frequent menstrual periods compared to CT women. They had lower blood pressure, lower triiodothyronine, and fasting glucose levels than CT women. Patients with AN had more disturbed body images and restricted diet patterns and also had higher neuroticism compared to CT women.ConclusionsThe patients with AN viewed their current body shape as fatter and had more restrained eating than the CT women. The results suggest that the disturbed body image may be a salient feature of AN differentiating from CT women, the underlying neural mechanism of which requires further study.