bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hyperaldosteronemia as a risk factor of renal dysfunction in patients with heart failure with preserved ejection fraction
Authors: Shevelok A. N. 
Published: November 2020

Objective: To assess the relationship between plasma aldosterone levels and renal function in patients with heart failure with preserved ejection fraction (HFpEF).Materials and methods: A cross-sectional study included 158 patients with confirmed HFpEF. Patients with primary hyperaldosteronism, edema syndrome, end stage renal disease and taking mineralocorticoid receptor antagonists were excluded. Renal function was assessed by determining daily urinary albumin excretion (UAE) and calculating the glomerular filtration rate (GFR). Plasma aldosterone was measured by enzyme immunoassay.Results: The patients were divided into two groups: 99 patients had normal (40-160 pg/ml) aldosterone plasma level (nAld) and 59 patients had high (> 160 pg/ml) aldosterone level (hAld). hAld patients had significantly higher UAE (median 342 mg/day [interquartile value 253; 453] versus 116 mg/day [32; 255], p < 0.001), and lower GFR (52 ml/min/1.73 m2 [46; 67.5] versus 66 ml/min/1.73 m2 [53; 79]) compared to nAld. The prevalence of impaired renal filtration function and severe albuminuria was higher in hAld group then in nAld (p < 0.001). In binomial logistic regression models adjusted for age, severity of HFpEF and comorbidities high aldosterone plasma level were independent risk factors of significant (< 60 ml/min/1.73 m2) decrease in GFR (odds ratio 4.25, 95% confidence interval 2.01-16.6) and very high (> 300 mg/day) albuminuria (odds ratio 2.23, 95% confidence interval 1.24-9.63).Conclusion: In HFpEF plasma aldosterone levels are closely related to renal function. Secondary hyperaldosteronism is associated with an increased risk of impaired renal filtration and severe albuminuria.
Variasi Presipitasi Pelarut pada Ekstraksi RNA dengan Metode Trizole dan Pengaruh terhadap Kemurnian RNA
Authors: Shaomianah Siti 
Published: November 2020

ABSTRAKVirus dengue ditularkan oleh nyamuk Aedes aegypti dan Aedes albopictus. Nyamuk-nyamuk tersebut endemik di sebagian besar daerah vektor-vektor itu muncul memiliki empat serotipe yang berbeda secara antigenik. Empat serotipe virus dengue: DENV-1, DENV-2, DENV-3 dan DENV-4. Penelitian ini bertujuan untuk mengetahui perbandingan pelarut yang baik terhadap virus Dengue RNA Surabaya tipe-1 (DENV-1). Pelarut yang digunakan adalah Isopropanol, dimethyl sulfoxide, 96% ethanol, methanol, acetone-Methanol (1: 1), dimethyl formamide, dan akuades. RNA diisolasi dengan metode TRIzol. TRIzol (atau TRI Reagent) adalah larutan satu fasa dari fenol dan guanidinium isothiocyanate yang secara bersamaan melarutkan bahan biologis dan mendenaturasi protein. Hasil dari penilitian ini adalah didapatkan pelarut presipitasi RNA terbaik adalah aseton-metanol (1:1), karena campuran pelarut tersebut memiliki konstanta dielektrik terendah. Studi ini menunjukkan bahwa pelarut aseton-metanol (1:1) dapat digunakan sebagai pelarut presipitasi untuk mengisolasi RNA
Overexpression of FAM83A Promotes Proliferation and Metastasis Via Activating Wnt/β-catenin Signaling Pathway in Head Neck Squamous Cell Carcinoma
Authors: Ji Huan , Song Haiyang , Zhang Wei , Wang Zeyu , Jiao Pengfei , Zheng Yang , Zheng Yangyu , Jiang Yue , Zhang Jinping , Xu Jiani , Li Xuan , Du Hongming , Ding Xu , Wu Heming , Zhong Yi 
Published: November 2020

Abstract
Purpose:This research aimed to investigate FAM83A expression in head and neck squamous cell carcinoma (HNSCC) and its function in the proliferation and metastasis.Materials and Methods:FAM83A mRNA and protein expression in HNSCC was detected via TCGA database, primary HNSCC samples, and cell lines. The associations between FAM83A expression and clinicopathologic variables were evaluated through tissue microarrays. Besides, FAM83A knockdown and overexpression cell lines were constructed to assess its biological function including cell growth and metastasis in vitro and the relationship between FAM83A and epithelial-mesenchymal transition (EMT) was explored. Furthermore, two models of xenograft tumors in nude mice were used to assess the tumorigenicity and metastasis ability of FAM83A in vivo.Results:In the present study, we discovered the FAM83A expression was overexpressed in the HNSCC samples and was significantly associated with tumor size, lymph node status, and pathological grades. Mechanically, FAM83A could promote HNSCC cell growth and metastasis by inducing EMT via activating Wnt/β-catenin signaling pathway. Rescue experiment demonstrated the inhibition of β-catenin could counteract FAM83A function and there is a bi-directional signaling loop between FAM83A and Wnt/β-catenin signaling pathways. Also, the FAM83A knockdown could suppress tumor growth and distant metastasis in HNSCC xenograft animal models.Conclusions:Taken together, we demonstrated that FAM83A is upregulated in HNSCC and it could promote cell growth and metastasis via activating Wnt/β-catenin pathway in HNSCC, which may provide a novel therapeutic target in HNSCC treatment.
Identification and validation of a novel prognosis prediction model in adrenocortical carcinoma by integrative bioinformatics analysis and statistics and machine learning
Authors: Yan Xin , Guo Zi-Xin , Yu Dong-Hu , Chen Chen , Liu Xiao-Ping , Yao Jie , Liu Tong-Zu , Li Sheng 
Published: November 2020

Abstract
Background: Adrenocortical carcinoma (ACC) is a rare malignancy with poor prognosis. Thus, we aimed to establish a gene signature to predict the prognosis for ACC. Methods: Firstly, “WGCNA” package was used to construct a co-expression network and screen key module. Secondly, survival associated genes were identified by performing survival analysis. Thirdly, regression models were constructed by using the Ridge, ELASTIC-NET, and LASSO methods. Time-dependent ROC analysis, Cox regression analysis, GSEA, DCA and nomogram were performed to validate the model. Fourthly, mutations and CNVs of genes in the model were explored. Finally, LDA, KNN, SVM, PPI network and meta-analysis were used screened and validated meaningful prognostic biomarkers.Results: Two key modules were selected and 93 survival associated genes were identified. Furthermore, 11 models were constructed and two models were further selected, which were validated in each dataset (training set, internal validation set, GSE19750, and GSE76021). Model 2 was further identified as the best model (training set: survival analysis: p < 0.0001; AUC: 0.92 at 1 year, 0.91 at 3 years and 0.95 at 5 years). In genes in the best model, MKI67 was altered most (12%). Six hub genes were further analyzed by constructing a PPI network and validated by meta-analysis. Conclusion: In summary, we constructed and validated a prognostic multi-gene model and six powerful prognostic biomarkers, which might be useful instruments for predicting the prognosis of ACC patients.
VOX Index– A New Parameter Using Ultrasound to Predict Time of Applying Noninvasive Ventilation
Authors: Wen Ruoxuan , Hu Xingshuo , Duan Zhimei , Fu Han , Gu Hongjun , Xie Lixin , Xie Fei 
Published: November 2020

Abstract
Background: Physicians have been utilizing respiratory status and arterial blood gases measurements of the patient for determining the need of noninvasive ventilation (NIV). There is actually no clear and accurate index to assess the appropriate time of using NIV. We hypothesized that diaphragmatic ultrasound in combination with arterial blood gases test can accurately predict when the patients require sequential NIV.Objective: To investigate the feasibility of using the new VOX (velocity oxygenation) index, combining the diaphragmatic ultrasound and oxygen pressure analyses, as a reliable assessment tool for sequential NIV in intensive care unit (ICU) patients. Method: A prospective study including patients admitted to ICU of People’s Liberation Army General Hospital First Medical Center Respiratory Department between August 1st 2018 and March 31st 2020. Patients received either continuous low-flow oxygen therapy or sequential NIV based on the physician judgement. The diaphragm movement distance, contraction time, and velocity were recorded and the E-T (excursion-time) index, VOX index, and arterial blood gases were compared. Result: The velocity of the diaphragmatic contraction (VD) was higher (3.29 vs. 1.89, P< 0.001) and P/F ratio was lower (161 vs. 264, P< 0.001) in patients receiving NIV. In predicting the need of utilizing NIV, VOX index showed the largest area under curve of ROC (AUC= 0.97) which was significantly greater than the AUC of VD and P/F ratio.Conclusion: VOX index serves as an accurate indicator of predicting the time of using NIV for ICU patients. It is recommended to switch from invasive ventilation to NIV when VOX< 109.89.
Myxoid liposarcoma with metastasis to the pancreas: a rare case report and review of the literature
Authors: Wang Ankang , Cong Peng , He Zhenxing , Qu Yueyu , Hu Tao , Cai Yu , Sun Bo , Chen Hao , Fu Wenguang , Peng Yong 
Published: November 2020

Abstract
BackgroundMetastasis of the pancreas to myxoid liposarcoma is very rare.Case presentationWe report a 43-year-old woman who underwent radical surgery for myxoid liposarcoma (MLPS) of the sigmoid colon 9 years earlier and for liposarcoma of the neck 7 years earlier. The lesion later metastasized to the pancreas, and we performed pancreaticoduodenectomy, which was pathologically confirmed as myxoid liposarcoma. Unfortunately, the patient died a year later from unexplained gastrointestinal bleeding.ConclusionsMyxoid liposarcoma is a very rare malignancy that carries a risk of invasion to the pancreas, and complete surgical resection is the best chance for clinical treatment.
Prognostic value of calculated tumor volume (cTV) in T4 or N3 advanced gastric cancer
Authors: Kim Ji-Hyun , Jun Kyonghwa , Chin Hyung-Min 
Published: November 2020

Abstract
Background: To evaluate the prognostic value of tumor volume in gastric cancer, we assess calculated tumor volume (cTV) based on pathologic reviews and investigate its correlation with long-term outcome of patients who underwent curative gastrectomy.Methods: In total, 574 gastric cancer patients who underwent curative gastrectomy in a single institution between January 2012 and December 2015 were enrolled and divided into subgroups (cTV group) according to cTV using pathologic tumor size and depth. The cutoff values of calculated cTV for subgroups were designated as 0-160.0 for the cTV1 group, 160.1-468.0 for the cTV2 group, and ≥ 468.1 for the cTV3 group.Results: Among enrolled patients, 403 (70.2%) were in the cTV1 group, 116 (20.2%) were in the cTV2 group, and 55 (9.6%) were in the cTV3 group. The 5-year overall survival rate of V groups were significantly different: 94.4% in cTV1, 86.2% in cTV2, and 69.3% in cTV3. In the univariate analysis, the cTV groups were associated with poor overall survival (OS) and disease-free survival (DFS) with age, tumor location, histologic type, Lauren classification, T stage, and N stage. In the multivariate analysis, the cTV group was an independent prognostic factor for OS (HR 2.50, P=0.049) and DFS (HR 2.25, P=0.044). In the subgroup analysis, there was a significant difference according to the cTV group in both OS and DFS in T4 and N3 gastric cancers. Conclusions: Tumor volume plays an additive role in predicting survival in patients with gastric cancer. Moreover, the calculated tumor volume is significant parameter associated with poor prognosis in T4 and N3 advanced gastric cancer regardless of TNM stage.
The Role of Social Norms in Institutional Delivery: Findings from A Survey in Makwanpur, Nepal
Authors: Paul Alicia M. , Rimal Rajiv N. , Nepal Shraddha , Lohani Jeevan 
Published: November 2020

Abstract
Background: Nepal has a maternal mortality ratio of 186 per 100,000 live births, the second highest in South Asia. Institutional delivery, or giving birth in a medical facility with a skilled birth attendant, can prevent up to 16-33% of maternal deaths. Despite recent progress, Nepal’s institutional delivery rate is only 54%. As maternal mortality remains high and institutional delivery low, a transition from a biomedical to a psychosocial approach is needed, through which normative beliefs may be an entry point. The purpose of this study is to examine the relationship between descriptive norms and institutional delivery among mothers in Makwanpur, Nepal. Methods: This study uses baseline data from the Rejoice Architecture Project, a quasi-experimental study conducted in the Makwanpur District in Nepal in 2020. Female Community Health Volunteers (FCHVs) recruited 356 women from catchment areas of six health facilities across three Palikas (equivalent to municipalities) and administered oral surveys. Participants were eligible if they were 18 years or older, had a child younger than 2 years, and lived within the catchment area of a study site. Logistic regression was used to determine associations between descriptive norms and institutional delivery, controlling for antenatal care (ANC) visits, demographics, and interpersonal communication (IPC) with husband.Results: Approximately 30% of participants perceived most women in their community gave birth at home compared to medical institutions and nearly 65% gave birth in an institution during their last delivery. Logistic regressions showed an association between descriptive norms and institutional delivery after controlling for external factors such that on average, women who perceived institutional delivery to be most common had 3.18 greater odds of giving birth in an institution. Conclusions: Findings from this study support the notion that social norms relate to actual birthing behavior, specifically in the context of institutional delivery. These findings contribute to the growing body of literature relating to institutional delivery and offer insights for a potential norms-based approach for increasing rates of institutional delivery and reducing maternal mortality in developing countries.
Comparison of hamstring and quadriceps strength after anatomical versus non-anatomical anterior cruciate ligament reconstruction: a retrospective cohort study
Authors: Jiang Hai , Zhang Lei , Zhang Rui-Ying , Zheng Qiu-Jian , Li Meng-Yuan 
Published: November 2020

Abstract
Background

Strength recovery of injured knee is an important parameter for patients who want to return to sport after anterior cruciate ligament reconstruction (ACLR). Comparison of muscle strength between anatomical and non-anatomical ACLR has not been reported.
Purpose

To evaluate the hamstring and quadriceps strength and clinical outcomes after anatomical and non-anatomical single-bundle ACLR.
Methods

Patients received unilateral primary single-bundle hamstring ACLR between January 2017 to January 2018 were recruited in this study. Patients were divided into anatomical reconstruction group (AR group) and non-anatomical reconstruction group (NAR group) according to femoral tunnel aperture position. The hamstring and quadriceps isokinetic strength including peak extension torque, peak flexion torque and H/Q ratio were measured at an angular velocity of 180°/s and 60°/s using an isokinetic dynamometer. The isometric extension and flexion torques were also measured. Hamstring and quadriceps strength were measured preoperatively and at 3, 6, and 12 months after surgery. Knee stability including Lachman test, pivot-shift test, and KT-1000 measurement and subjective knee function including International Knee Documentation Committee (IKDC) and Lysholm scores were evaluated during the follow-up.
Results

Seventy-two patients with an average follow-up of 30.4 months (range, 24–35 months) were included in this study. Thirty-three were in AR group and 39 in NAR group. The peak knee flexion torque was significant higher in AR group at 180°/s and 60°/s (P < 0.05 for both velocity) at 6 months postoperatively and showed no difference between the two groups at 12 months postoperatively. The isometric knee extension torque was significant higher in AR group at 6 months postoperatively (P < 0.05) and showed no difference between the two groups at 12 months postoperatively. No significant differences between AR group and NAR group were found regarding knee stability and subjective knee function evaluations at follow-up.
Conclusion

Compared with non-anatomical ACLR, anatomical ACLR showed a better recovery of hamstring and quadriceps strength at 6 months postoperatively. However, the discrepancy on hamstring and quadriceps strength between the two techniques vanished at 1 year postoperatively.
A novel method to establish the rabbit model of knee Osteoarthritis: Intra-articular Injection of SDF-1 induces OA
Authors: LI CANZHANG , HE YINHONG , LI YANLIN , WANG GUOLIANG , LIU DEJIAN , CAI GUOFENG , HE CHUAN 
Published: November 2020

Abstract
BackgroundAnimal model of Knee Osteoarthritis (OA) is the primary testing methodology for studies on pathogenic mechanisms and therapies of human OA disease. Recent major modeling methods are divided into artificially induced and spontaneous. However, these methods have some disadvantages of slow progression, high cost and no correlation with the pathogenesis of OA.MethodsOur studies attempted to find a rapid, easy, and consistent with the natural pathological process of OA modeling method by intra-articular injection of SDF-1 (stromal cell-derived factor 1, a chemokine naturally occurring during the pathological process of human OA) in the rabbit knee. After induction we collected cartilage specimens from the medial femoral condyle to undergo macroscopic, histological, immunohistochemical, and biochemical evaluations. Meanwhile, compared with Hulth surgical method to evaluate its efficacy.ResultsMacroscopic observation and Mankin’s score of histological staining exhibited typical features of middle stage OA cartilage in SDF-1 injected groups. Immunohistochemically, the positive expression of IL-1 (interleukin-1) and TNF-α(tumor necrosis factor α)was earlier and higher in high dose SDF-1 group than surgical group. The levels of matrix metalloproteinases (MMPs) in synovial fluid and in chondrocytes significantly increased, and type II collagen (COLⅡ) and aggrecan (ACAN) expressions decreased in SDF-1 injected group following the extension of time and increase of SDF-1 concentration.ConclusionsOur data indicated intra-articular injection of SDF-1 (40ug/kg, three times for 12weeks) can induce rabbit knee OA model successfully, more rapidly and easily than traditional surgical modeling. The study provides a further option for the establishment of knee OA animal model.
Extrachromosomal Circular DNAs are Common and Functional in Esophageal Squamous Cell Carcinoma
Authors: Sun Zhenguo , Ji Na , Zhao Renchang , Liang Jinghui , Jiang Jin , Tian Hui 
Published: November 2020

Abstract
BackgroundEsophageal squamous cell carcinoma (ESCC) is the leading cause of cancer-related mortality worldwide. Extrachromosomal circular DNAs (eccDNAs), especially the more-spread smaller type, were rarely studied before. Recently, the distribution of eccDNAs was reported to be distinguished in different tumor cell lineage or different condition of lung cancer. However, there was no exploration of eccDNA distribution and function in ESCC. MethodsThe eccDNAs from 3 surgical matched ESCC tissues were extracted and amplified after removing linear DNA / mitochondrial circular DNA and rolling circle amplification. High-throughput eccDNA sequencing and subsequent bioinformatics analysis was performed to study the distribution pattern and the level of eccDNAs in these matched tissues. Gene ontology and KEGG pathway analysis based on the genes associated with the eccDNAs at differential level were performed. 5 up-regulated and 5 down-regulated candidate eccDNAs were selected to be validated by routine PCR, TOPO-TA Cloning and Sanger sequencing. The nucleotides flanking eccDNA junctions were analyzed to explore the mechanism of eccDNA formation.Results184557 eccDNAs were identified. The overall length distribution was from 33bp to 968842bp, with the peak at ~360bp. They were originated mainly from 5’-untranslated regions, 3’-untranslated region, and rarely from exons, introns, LINE or Alu repeat region. The distribution patterns of the eccDNAs, such as chromosome distribution, length distribution and genomic annotation, were similar between ESCC and matched normal epithelium. Nevertheless, 16031 eccDNAs were found to be at differential level between ESCC and matched normal epithelium, including 10126 up-regulated eccDNAs and 5905 down-regulated eccDNAs. GO analysis and KEGG pathway analysis showed enriched pathways in cancer, MAPK pathway, GTPase related activity and cytoskeleton function et al. PCR, TOPO-TA Cloning and Sanger sequencing validated the junctional sites of 5 up-regulated candidate eccDNAs and 4 other unexpected eccDNAs. Repeat nucleotide pattern between the position flanking the start site and that flanking the end site was detected.ConclusionThis study firstly demonstrated genome-wide presence of eccDNAs, explored the eccDNAs at differential level and revealed potential mechanism of eccDNAs in ESCC, thus suggesting potential clinical utility in ESCC and increasing our understanding about genome plasticity.
CircFAM114A2 Inhibits Bladder Cancer Proliferation and Promotes Cisplatin Sensitivity via the miR-222-3p/P27 and miR-146a-5p/P21 Cascades
Authors: Lu Jiancheng , Zhou Zijian , Wang Jingzi , Yang Xiao , Yu Hao , Han Jie , Feng Dexiang , Yuan Baorui , Wu Qikai , Li Pengchao , Lu Qiang , Yang Haiwei 
Published: November 2020

Abstract
Background: Circular RNAs (circRNAs) are noncoding RNAs that have the structure of a covalently closed loop. Increasing data has proved that circRNA can influence the development and progression of tumors. CircFAM114A2 is generated from several exons of FAM114A2. However, the function and mechanisms of circFAM114A2 in bladder cancer (BCa) remain unclear. Methods: Here, to elucidate the potential roles of circFAM114A2 in BCa, we conducted RNA-sequencing on 5 pairs of BCa samples and screened for circRNAs. CircRNAs, microRNAs (miRNAs) and mRNAs, as well as levels of P27 and P21, in human cells and tissues were detected by qRT-PCR and western blot, respectively. CircRNA-miRNA interactions and miRNA-downstream mRNAs interactions were investigated by RNA pull-down assay and fluorescence in situ hybridization (FISH) or luciferase reporter assays, respectively. Then, the function of circFAM114A2 in BCa was explored using cell proliferation, cell cycle and tumorigenesis assays in nude mice. Finally, the function of circFAM114A2 in cisplatin chemo-sensitivity in BCa was detected by IC50 and tumor formation of xenograft in cisplatin-treated nude mice. Results: We discovered that circFAM114A2 levels were decreased in BCa cell lines and tissues. According to follow-up data, BCa patients with higher circFAM114A2 expression had better survival. Importantly, the levels of circFAM114A2 were associated with the histological grade of BCa. Overexpression of circFAM114A2 inhibited cell proliferation and increased sensitivity to cisplatin chemotherapy. Mechanistically, circFAM114A2 directly sponged miR-222-3p/miR-146a-5p and subsequently influenced the expression of the downstream target genes P27/P21, which, in turn, inhibited progression of BCa. Conclusion: In conclusion, circFAM114A2 acted as a tumor suppressor through a novel circFAM114A2/miR-222-3p/P27 and circFAM114A2/miR-146a-5p/P21 pathway. CircFAM1142 has therefore great potential as a prognostic biomarker and therapeutic target for BCa.