bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adverse childhood experiences and household food insecurity among children aged 0–5 years in the USA
Authors: Baiden Philip , LaBrenz Catherine A , Thrasher Shawndaya , Asiedua-Baiden Gladys , Harerimana Boniface 
Published: September 2020

Abstract

Objective:
Although studies have examined the association between adverse childhood experiences (ACE) and health and mental health outcomes, few studies have investigated the association between ACE and household food insecurity among children aged 0–5 years in the USA. The objective of this study is to investigate the association between ACE and household food insecurity among children aged 0–5 years.

Design:
The data used in this study came from the 2016–2017 National Survey of Children’s Health. Data were analysed using multinomial logistic regression with household food insecurity as the outcome variable.

Setting:
United States.

Participants:
An analytic sample of 17 543 children aged 0–5 years (51·4% boys).

Results:
Of the 17 543 respondents, 83·7% experienced no childhood adversity. About one in twenty (4·8%) children experienced moderate-to-severe food insecurity. Controlling for other factors, children with one adverse childhood experience had 1·43 times the risk of mild food insecurity (95 % CI 1·25, 1·63) and 2·33 times the risk of moderate-to-severe food insecurity (95 % CI 1·84, 2·95). The risk of mild food insecurity among children with two or more ACE was 1·5 times higher (95 % CI 1·24, 1·81) and that of moderate-to-severe food insecurity was 3·96 times higher (95 % CI 3·01, 5·20), when compared with children with no childhood adversity.

Conclusion:
Given the critical period of development during the first few years of life, preventing ACE and food insecurity and early intervention in cases of adversity exposure is crucial to mitigate their negative impact on child development.
A systematic review of instruments measuring grief after perinatal loss and factors associated with grief reactions
Authors: Setubal M.S. , Bolibio R. , Jesus R.C. , Benute G.G. , Gibelli M.A. , Bertolassi N. , Barbosa T. , Gomes A. , Figueiredo F. , Ferreira R. , Francisco R. , Stein Bernardes L. 
Published: September 2020

AbstractObjectiveExpectant parents who live through perinatal loss experience intense grief, which is not always acknowledged or accepted. A screening tool to detect bereaved parents’ grief reactions can guide professionals, including perinatal palliative care teams, to provide follow-up for those in need. This review's goal is to identify and synthesize the international published literature on existent instruments specifically measuring the grieving process after any perinatal loss and to identify factors that could moderate grief reactions.MethodSystematic review (PROSPERO # CRD42018092555) with critical synthesis. PUBMED, Cochrane, and PsycINFO databases were searched in English language articles using the keywords “perinatal” AND (“grief” OR “bereavement” OR mourning) AND (“scale” OR “questionnaire” OR “measure” OR “assessment”) up to May 2018. Eligibility criteria included every study using a measure to assess perinatal grief after all kinds of perinatal losses, including validations and translations to other languages and interventions designed to alleviate grief symptoms.ResultsA total of 67 papers met inclusion criteria. Seven instruments measuring perinatal grief published between 1984 and 2002 are described. The Perinatal Grief Scale (PGS) was used in 53 of the selected studies. Of those, 39 analyzed factors associated with grief reactions. Six articles used PGS scores to evaluate pre- and post-bereavement interventions. Studies in English language only might have limited the number of articles.Significance of resultsThe PGS is the most used standardized measures to assess grief after perinatal loss. All parents living through any kind of perinatal loss should be screened.
The Anomalous 2019 Antarctic Ozone Hole in the GEOS Constituent Data Assimilation System With MLS Observations
Authors: Wargan Krzysztof , Weir Brad , Manney Gloria L. , Cohn Stephen E. , Livesey Nathaniel J. 
Published: September 2020

AbstractA rare disturbance of the stratospheric Antarctic polar vortex in September 2019 led to a significantly higher than usual polar total ozone column. We use assimilation of ozone, HCl, and N2O data from the Microwave Limb Sounder with the Global Earth Observing System (GEOS) Constituent Data Assimilation System driven by reanalysis meteorology to study the evolution of the 2019 Antarctic polar ozone. We find that the maximum 2019 ozone hole area was near 10 × 106 km2, and as little as 20% of that in 2018 in mid‐September. However, the magnitude of vortex‐averaged chemical ozone depletion was not significantly different between the 2 years despite earlier chlorine deactivation in 2019. The assimilation results show that most of the differences between 2018 and 2019 Antarctic ozone resulted from two factors: (1) the geometry of the 2019 vortex, with ozone‐rich middle‐stratospheric air masses overlying the lower portion of the vortex and leading to a significant reduction of the total column, and (2) significantly reduced vortex volume. The anomalously small ozone hole of 2019 was comparable in size to the record breaking 2002 case and the mechanisms responsible were similar in the two cases. While the 2019 sudden stratospheric warming is classified as minor, its impact on ozone was very significant.
Assimilation of Sparse Continuous Near‐Earth Weather Measurements by NECTAR Model Morphing
Authors: Galkin I. A. , Reinisch B. W. , Vesnin A. M. , Bilitza D. , Fridman S. , Habarulema J. B. , Veliz O. 
Published: September 2020

AbstractNon‐linear Error Compensation Technique with Associative Restoration (NECTAR) is a novel approach to the assimilation of fragmentary sensor data to produce a global nowcast of the near‐Earth space weather. NECTAR restores missing information by iteratively transforming (“morphing”) an underlying global climatology model into agreement with currently available sensor data. The morphing procedure benefits from analysis of the inherent multiscale diurnal periodicity of the geosystems by processing 24‐hr time histories of the differences between measured and climate‐expected values at each sensor site. The 24‐hr deviation time series are used to compute and then globally interpolate the diurnal deviation harmonics. NECTAR therefore views the geosystem in terms of its periodic planetary‐scale basis to associate observed fragments of the activity with the grand‐scale weather processes of the matching variability scales. Such approach strengthens the restorative capability of the assimilation, specifically when only a limited number of observatories is available for the weather nowcast. Scenarios where the NECTAR concept works best are common in planetary‐scale near‐Earth weather applications, especially where sensor instrumentation is complex, expensive, and therefore scarce. To conduct the assimilation process, NECTAR employs a Hopfield feedback recurrent neural network commonly used in the associative memory architectures. Associative memories mimic human capability to restore full information from its initial fragments. When applied to the sparse spatial data, such a neural network becomes a nonlinear multiscale interpolator of missing information. Early tests of the NECTAR morphing reveal its enhanced capability to predict system dynamics over no‐data regions (spatial interpolation).
Retracted: Identification of de novo mutations in prenatal neurodevelopment-associated genes in schizophrenia in two Han Chinese patient-sibling family-based cohorts
Authors: Jiang Shan , Zhou Daizhan , Wang Yin-Ying , Jia Peilin , Wan Chunling , Li Xingwang , He Guang , Cao Dongmei , Jiang Xiaoqian , Kendler Kenneth S. , Tsuang Ming , Mize Travis , Wu Jain-Shing , Lu Yimei , He Lin , Chen Jingchun , Zhao Zhongming , Chen Xiangning 
Published: September 2020

AbstractSchizophrenia (SCZ) is a severe psychiatric disorder with a strong genetic component. High heritability of SCZ suggests a major role for transmitted genetic variants. Furthermore, SCZ is also associated with a marked reduction in fecundity, leading to the hypothesis that alleles with large effects on risk might often occur de novo. In this study, we conducted whole-genome sequencing for 23 families from two cohorts with unaffected siblings and parents. Two nonsense de novo mutations (DNMs) inGJC1andHIST1H2ADwere identified in SCZ patients. Ten genes (DPYSL2, NBPF1, SDK1, ZNF595, ZNF718, GCNT2, SNX9, AACS, KCNQ1, andMSI2) were found to carry more DNMs in SCZ patients than their unaffected siblings by burden test. Expression analyses indicated that these DNM implicated genes showed significantly higher expression in prefrontal cortex in prenatal stage. The DNM in theGJC1gene is highly likely a loss function mutation (pLI = 0.94), leading to the dysregulation of ion channel in the glutamatergic excitatory neurons. Analysis of rare variants in independent exome sequencing dataset indicates thatGJC1has significantly more rare variants in SCZ patients than in unaffected controls. Data from genome-wide association studies suggested that common variants in theGJC1gene may be associated with SCZ and SCZ-related traits. Genes co-expressed withGJC1are involved in SCZ, SCZ-associated pathways, and drug targets. These evidences suggest thatGJC1may be a risk gene for SCZ and its function may be involved in prenatal and early neurodevelopment, a vulnerable period for developmental disorders such as SCZ.
Sulforaphene inhibits esophageal cancer progression via suppressing SCD and CDH3 expression, and activating the GADD45B-MAP2K3-p38-p53 feedback loop
Authors: Han Sichong , Wang Yandong , Ma Jie , Wang Zhe , Wang Hui-Min David , Yuan Qipeng 
Published: September 2020

AbstractEsophageal cancer is one of the most common cancer with limited therapeutic strategies, thus it is important to develop more effective strategies to against it. Sulforaphene (SFE), an isothiocyanate isolated from radish seeds, was proved to inhibit esophageal cancer progression in the current study. Flow cytometric analysis showed SFE induced cell apoptosis and cycle arrest in G2/M phase. Also, scrape motility and transwell assays presented SFE reduced esophageal cancer cell metastasis. Microarray results showed the influence of SFE on esophageal cancer cells was related with stearoyl-CoA desaturase (SCD), cadherin 3 (CDH3), mitogen-activated protein kinase kinase 3 (MAP2K3) and growth arrest and DNA damage inducible beta (GADD45B). SCD and CDH3 could promote esophageal cancer metastasis via activating the Wnt pathway, while the latter one was involved in a positive feedback loop, GADD45B-MAP2K3-p38-p53, to suppress esophageal cancer growth. GADD45B was known to be the target gene of p53, and we proved in this study, it could increase the phosphorylation level of MAP2K3 in esophageal cancer cells, activating p38 and p53 in turn. SFE treatment elevated MAP2K3 and GADD45B expression and further stimulated this feedback loop to better exert antitumor effect. In summary, these results demonstrated that SFE had the potential for developing as a chemotherapeutic agent because of its inhibitory effects on esophageal cancer metastasis and proliferation.
RGS12 is required for the maintenance of mitochondrial function during skeletal development
Authors: Yuan Gongsheng , Yang Shuting , Liu Min , Yang Shuying 
Published: September 2020

AbstractMitochondrial morphology and function are crucial for tissue homeostasis, such as for skeletal development, but the cellular and molecular mechanisms remain unclear. Here, we provide evidence that regulator of G-protein signaling 12 (RGS12) is present in the mitochondria of primary chondrocytes and cartilage tissues. Deletion of RGS12 in type II collagen-positive cells led to a significant decrease in mitochondrial number, membrane potential, and oxidative phosphorylation function. Mechanistically, RGS12 promoted the function of ATP5A as an enhancer of tyrosine phosphorylation. Mice with RGS12 deficiency in the chondrocyte lineage showed serious body retardation, decreased bone mass, and chondrocyte apoptosis due to the defective activity of ATP synthase. To our knowledge, this is the first report that RGS12 is required for maintaining the function of mitochondria, which may allow it to orchestrate responses to cellular homeostasis.
Woodland strawberry WRKY71 acts as a promoter of flowering via a transcriptional regulatory cascade
Authors: Lei Yingying , Sun Yiping , Wang Baotian , Yu Shuang , Dai Hongyan , Li He , Zhang Zhihong , Zhang Junxiang 
Published: September 2020

AbstractThe WRKY proteins are a large family of transcription factors that play important roles in stress responses and plant development. However, the roles of most WRKYs in strawberry are not well known. In this study, FvWRKY71 was isolated from the woodland strawberry ‘Ruegen’. FvWRKY71 was highly expressed in the shoot apex and red fruit. Subcellular localization analysis showed that FvWRKY71 was located in the nucleus. Transactivation analysis showed that FvWRKY71 presented transcriptional activation activity in yeast. Overexpression of FvWRKY71 in Arabidopsis and woodland strawberry revealed early flowering in the transgenic plants compared with the wild-type control. Gene expression analysis indicated that the transcript levels of the flowering time and development integrator genes AP1, LFY, FT, AGL42, FUL, FPF1, SEP1, SEP2, and SEP3 were increased in FvWRKY71-overexpressing Arabidopsis and strawberry plants compared with the wild-type controls, which may result in accelerated flowering in transgenic plants. Furthermore, FvWRKY71 was proven to directly bind to the W-boxes (TTGACT/C) of the FvFUL, FvSEP1, FvAGL42, FvLFY, and FvFPF1 promoters in vitro and in vivo. Taken together, our results reveal a transcriptional regulatory cascade of FvWRKY71 involved in promoting flowering in woodland strawberry.
The GAMYB-like gene SlMYB33 mediates flowering and pollen development in tomato
Authors: Zhang Yan , Zhang Bo , Yang Tongwen , Zhang Jie , Liu Bin , Zhan Xiangqiang , Liang Yan 
Published: September 2020

AbstractGAMYBs are positive GA signaling factors that exhibit essential functions in reproductive development, particularly in anther and pollen development. However, there is no direct evidence of the regulation of any GAMYB in these biological processes in tomato (Solanum lycopersicum). Here, we identified a tomato GAMYB-like gene, SlMYB33, and characterized its specific roles. SlMYB33 is predominately expressed in the stamens and pistils. During flower development, high mRNA abundance of SlMYB33 is detected in both male and female organs, such as microspore mother cells, anthers, pollen grains, and ovules. Silencing of SlMYB33 leads to delayed flowering, aberrant pollen viability, and poor fertility in tomato. Histological analyses indicate that SlMYB33 exerts its function in pollen development in the mature stage. Further transcriptomic analyses imply that the knockdown of SlMYB33 significantly inhibits the expression of genes related to flowering in shoot apices, and alters the transcription of genes controlling sugar metabolism in anthers. Taken together, our study suggests that SlMYB33 regulates tomato flowering and pollen maturity, probably by modulating the expression of genes responsible for flowering and sugar metabolism, respectively.
The Phoebe genome sheds light on the evolution of magnoliids
Authors: Chen Shi-Pin , Sun Wei-Hong , Xiong Yuan-Fang , Jiang Yu-Ting , Liu Xue-Die , Liao Xing-Yu , Zhang Di-Yang , Jiang Shu-Zhen , Li Yu , Liu Bin , Ma Liang , Yu Xia , He Li , Liu Bao , Feng Jin-Lin , Feng Li-Zhen , Wang Zhi-Wen , Zou Shuang-Quan , Lan Si-Ren , Liu Zhong-Jian 
Published: September 2020

AbstractLauraceae includes the genusPhoebe, and the family is linked to the evolution of magnoliids. We sequenced the genome ofPhoebe bourneiNanmu. The assembled genome size was 989.19 Mb, with a contig N50 value of 2.05 Mb. A total of 28,198 protein-coding genes were annotated inP. bournei. Whole-genome duplication (WGD) analysis showed that Lauraceae has experienced two WGD events; the older WGD event occurred just before the divergence of Lauraceae and Magnoliales, and the more recent WGD was shared by all lineages of Lauraceae. The phylogenetic tree showed that magnoliids form a sister clade to monocots and eudicots. We also identified 63 MADS-box genes, includingAGL12-like genes that may be related to the regulation ofP. bourneiroots andFIN219-like genes encoding GH3 proteins, which are involved in photomorphogenesis.SAUR50-like genes involved in light signal-mediated pedicel or stem development were also identified. FourATMYB46-and threePtrEPSP-homologous genes related to lignin biosynthesis were identified. These genes may be associated with the formation of straight trunks inP. bournei. Overall, theP. bourneireference genome provides insight into the origin, evolution, and diversification ofPhoebeand other magnoliids.
American mastodon mitochondrial genomes suggest multiple dispersal events in response to Pleistocene climate oscillations
Authors: Karpinski Emil , Hackenberger Dirk , Zazula Grant , Widga Chris , Duggan Ana T. , Golding G. Brian , Kuch Melanie , Klunk Jennifer , Jass Christopher N. , Groves Pam , Druckenmiller Patrick , Schubert Blaine W. , Arroyo-Cabrales Joaquin , Simpson William F. , Hoganson John W. , Fisher Daniel C. , Ho Simon Y. W. , MacPhee Ross D. E. , Poinar Hendrik N. 
Published: September 2020

AbstractPleistocene glacial-interglacial cycles are correlated with dramatic temperature oscillations. Examining how species responded to these natural fluctuations can provide valuable insights into the impacts of present-day anthropogenic climate change. Here we present a phylogeographic study of the extinct American mastodon (Mammut americanum), based on 35 complete mitochondrial genomes. These data reveal the presence of multiple lineages within this species, including two distinct clades from eastern Beringia. Our molecular date estimates suggest that these clades arose at different times, supporting a pattern of repeated northern expansion and local extirpation in response to glacial cycling. Consistent with this hypothesis, we also note lower levels of genetic diversity among northern mastodons than in endemic clades south of the continental ice sheets. The results of our study highlight the complex relationships between population dispersals and climate change, and can provide testable hypotheses for extant species expected to experience substantial biogeographic impacts from rising temperatures.
Astrocyte-mediated switch in spike timing-dependent plasticity during hippocampal development
Authors: Falcón-Moya Rafael , Pérez-Rodríguez Mikel , Prius-Mengual José , Andrade-Talavera Yuniesky , Arroyo-García Luis E. , Pérez-Artés Rocío , Mateos-Aparicio Pedro , Guerra-Gomes Sónia , Oliveira João Filipe , Flores Gonzalo , Rodríguez-Moreno Antonio 
Published: September 2020

AbstractPresynaptic spike timing-dependent long-term depression (t-LTD) at hippocampal CA3-CA1 synapses is evident until the 3rd postnatal week in mice, disappearing during the 4th week. At more mature stages, we found that the protocol that induced t-LTD induced t-LTP. We characterized this form of t-LTP and the mechanisms involved in its induction, as well as that driving this switch from t-LTD to t-LTP. We found that this t-LTP is expressed presynaptically at CA3-CA1 synapses, as witnessed by coefficient of variation, number of failures, paired-pulse ratio and miniature responses analysis. Additionally, this form of presynaptic t-LTP does not require NMDARs but the activation of mGluRs and the entry of Ca2+ into the postsynaptic neuron through L-type voltage-dependent Ca2+ channels and the release of Ca2+ from intracellular stores. Nitric oxide is also required as a messenger from the postsynaptic neuron. Crucially, the release of adenosine and glutamate by astrocytes is required for t-LTP induction and for the switch from t-LTD to t-LTP. Thus, we have discovered a developmental switch of synaptic transmission from t-LTD to t-LTP at hippocampal CA3-CA1 synapses in which astrocytes play a central role and revealed a form of presynaptic LTP and the rules for its induction.
Dual roles of neutrophils in metastatic colonization are governed by the host NK cell status
Authors: Li Peishan , Lu Ming , Shi Jiayuan , Hua Li , Gong Zheng , Li Qing , Shultz Leonard D. , Ren Guangwen 
Published: September 2020

AbstractThe role of neutrophils in solid tumor metastasis remains largely controversial. In preclinical models of solid tumors, both pro-metastatic and anti-metastatic effects of neutrophils have been reported. In this study, using mouse models of breast cancer, we demonstrate that the metastasis-modulating effects of neutrophils are dictated by the status of host natural killer (NK) cells. In NK cell-deficient mice, granulocyte colony-stimulating factor-expanded neutrophils show an inhibitory effect on the metastatic colonization of breast tumor cells in the lung. In contrast, in NK cell-competent mice, neutrophils facilitate metastatic colonization in the same tumor models. In an ex vivo neutrophil-NK cell-tumor cell tri-cell co-culture system, neutrophils are shown to potentially suppress the tumoricidal activity of NK cells, while neutrophils themselves are tumoricidal. Intriguingly, these two modulatory effects by neutrophils are both mediated by reactive oxygen species. Collectively, the absence or presence of NK cells, governs the net tumor-modulatory effects of neutrophils.
Dearomative 1,4-difunctionalization of naphthalenes via palladium-catalyzed tandem Heck/Suzuki coupling reaction
Authors: Zhou Bo , Wang Hongliang , Cao Zhong-Yan , Zhu Jia-Wen , Liang Ren-Xiao , Hong Xin , Jia Yi-Xia 
Published: September 2020

AbstractDearomative functionalization reactions represent an important strategy for the synthesis of valuable three-dimensional molecules from simple planar aromatics. Naphthalene is a challenging arene towards transition-metal-catalyzed dearomative difunctionalization reactions. Reported herein is an application of naphthalene as a masked conjugated diene in a palladium-catalyzed dearomative 1,4-diarylation or 1,4-vinylarylation reaction via tandem Heck/Suzuki sequence. Three types of 1,4-dihydronaphthalene-based spirocyclic compounds are achieved in excellent regio- and diastereoselectivities. Key to this transformation is the inhibition of a few competitive side reactions, including intramolecular naphthalenyl C-H arylation, intermolecular Suzuki cross-coupling, dearomative 1,2-difunctionalization, and dearomative reductive-Heck reaction. Density functional theory (DFT) calculations imply that the facile exergonic dearomative insertion of a naphthalene double bond disrupts the sequence of direct Suzuki coupling, leading to the tandem Heck/Suzuki coupling reaction. The observed regioselectivity towards 1,4-difunctionalization is due to the steric repulsions between the introduced aryl group and the spiro-scaffold in 1,2-difunctionalization.
Site-selective C-H hydroxylation of pentacyclic triterpenoids directed by transient chiral pyridine-imino groups
Authors: Mu Tong , Wei Bingcheng , Zhu Dapeng , Yu Biao 
Published: September 2020

AbstractPentacyclic triterpenoids (PTs) constitute one of the biggest families of natural products, many with higher oxidation state at the D/E rings possess a wide spectrum of biological activties but are poorly accessible. Here we report a site-selective C-H hydroxylation at the D/E rings of PTs paving a way toward these important natural products. We find that Schönecker and Baran’s Cu-mediated aerobic oxidation can be applied and become site-selective on PT skeletons, as being effected unexpectedly by the chirality of the transient pyridine-imino directing groups. To prove the applicability, starting from the most abundant triterpenoid feedstock oleanane, three representative saponins bearing hydroxyl groups at C16 or C22 are expeditiously synthesized, and barringtogenol C which bears hydroxyl groups at C16, C21, and C22 is synthesized via a sequential hydroxylation as the key steps.