bluuebunny/crossref_metadata_2025_split huggingface dataset:
NFATc2-dependent epigenetic upregulation of CXCL14 is involved in the development of neuropathic pain induced by paclitaxel
Authors: Liu Meng , Zhang Su-Bo , Luo Yu-Xuan , Yang Yan-Ling , Zhang Xiang-Zhong , Li Bo , Meng Yan , Chen Yuan-Jie , Guo Rui-Xian , Xiong Yuan-Chang , Xin Wen-Jun , Li Dai 
Published: June 2020

Abstract
Background: The major dose-limiting toxicity of paclitaxel, one of the most commonly used drugs to treat solid tumor, is painful neuropathy. However, the molecular mechanisms underlying paclitaxel-induced painful neuropathy are largely unclarified. Methods: Paw withdrawal threshold was measured in the rats following intraperitoneal injection of paclitaxel. The qPCR, western blotting, protein or chromatin immunoprecipitation, ChIP-seq identification of NFATc2 binding sites, microarray analysis were performed to explore the molecular mechanism. Results: We found that paclitaxel treatment increased the expression of NFATc2 in the spinal dorsal horn, and knockdown of NFATc2 with NFATc2 siRNA significantly attenuated the mechanical allodynia induced by paclitaxel. Further binding site analysis utilizing ChIP-seq assay combining with gene expression profile revealed a shift of NFATc2 binding site closer to TSS of target genes in dorsal horn after paclitaxel treatment. We further found that NFATc2 occupancy directly upregulated the chemokine CXCL14 expression in dorsal horn, which was mediated by enhanced interaction between NFATc2 and p300 and consequently increased acetylation of histone H4 in CXCL14 promoter region. Also, knockdown of CXCL14 in dorsal horn significantly attenuated mechanical allodynia induced by paclitaxel. Conclusion: These results suggested that enhanced interaction between p300 and NFATc2 mediated the epigenetic upregulation of CXCL14 in the spinal dorsal horn, which contributed to the chemotherapeutic paclitaxel-induced chronic pain.
Successful use of anakinra in a patient with COVID-19- associated pericarditis
Authors: Karadeniz Hazan , Yamak Betül Ayça , Özger Hasan Selçuk , Sezenöz Burak , Tufan Abdurrahman , Emmi Giacomo 
Published: June 2020

Abstract
Since its recognition in December 2019 as a cause of pneumonia, SARS-CoV-2 infection has rapidly spread globally, causing a pandemic. Although it is an infectious disease, several distinct manifestations have been described in the setting of COVID-19, which result from the immune perturbations caused by this new Coronavirus. The heart is frequently involved in COVID-19 and is associated with severe outcomes. Pericarditis is rarely reported in COVID-19 patients and no reports on its treatment and outcome are available so far. Herein, we present a 33-year-old man with COVID-19-related pericarditis (COVID-19-RP) who was unresponsive to colchicine and nonsteroidal anti-inflammatory drugs, but was successfully treated with the IL-1R antagonist anakinra.
​Analysis of the Iranian maternal mortality surveillance system and providing system improvement strategies: Study protocol for strategy formulation
Authors: Beigi Marjan , Sadatmahalleh Shahideh Jahanian , Changizi Nasrin , Mohammadi Eesa , Kazemi Ashraf 
Published: June 2020

Abstract
Background
The implementation of the maternal mortality surveillance system in Iran has significantly reduced the incidence of maternal mortality. However, the pattern of the causes of the mortalities, which has remained constant over the years, are still concerning. This study aimed to explain the experiences of the actors of the Iranian maternal mortality surveillance and provide strategies for improving this system.
Methods
This research is a qualitative study to develop strategies, that will be conducted in two phases. In the first phase, purposive sampling will be performed, and the data will be collected based on the experiences of the Iranian maternal mortality surveillance system actors in Iran's Ministry of Health and the selected universities (Shiraz, Isfahan, Tehran, Zahedan, Alborz, Shahrekord) through semi-structured interviews. Moreover, during this phase, some part of the data will be collected through random participation of the researcher in some maternal mortality committees of the selected universities. In order to carry out the second phase, a panel of experts will be set up to discuss the best strategies for improving the Iranian maternal mortality surveillance by considering the above results.
Discussion
The analysis of maternal mortality surveillance system needs to evaluate the experiences of the actors who are the policymakers of this system and can be effective in identifying its challenges. This analysis and formulation of the subsequent strategies can lead to maternal health indicators remaining within the range of international standards or even beyond those standards in Iranian universities and countries with similar surveillance system.
Risk Factors for the Critical Illness in SARS-CoV-2 Infection: a Multicenter Retrospective Cohort Study
Authors: Cheng Sijing , Wu Dingfeng , Li Jie , Zou Yifeng , Wan Yunle , Shen Lihan , Zhu Lixin , Shi Mang , Hou Linlin , Xu Tao , Jiao Na , Li Yichen , Huang Yibo , Tang Zhipeng , Xu Mingwei , Jiang Shusong , Li Maokun , Yan Guangjun , Lan Ping , Zhu Ruixin 
Published: June 2020

Abstract
BackgroundPrior studies reported that 5~32% COVID-19 patients were critically ill, a situation that poses great challenge for the management of the patients and ICU resources. We aim to identify independent risk factors to serve as prediction markers for critical illness of SARS-CoV-2 infection. Methods Fifty-two critical and 200 non-critical SARS-CoV-2 nucleic acid positive patients hospitalized in 15 hospitals outside Wuhan from January 19 to March 6, 2020 were enrolled in this study. Multivariable logistic regression and LASSO logistic regression were performed to identify independent risk factors for critical illness.ResultsAge older than 60 years, dyspnea, respiratory rate > 24 breaths per min, leukocytosis >9.5 X109/L, neutrophilia >6.3 X109/L, lymphopenia <1.1 X109/L, neutrophil-to-lymphocyte ratio >3.53, fibrinogen >4g/L, d-dimer >0.55 µg/mL, blood urea nitrogen >7.1 mM, elevated aspartate transaminase, elevated alanine aminotransferase, total bilirubin >21 µM, and Sequential Organ Failure Assessment (SOFA) score ≥2 were identified as risk factors for critical illness. LASSO logistic regression identified the best combination of risk factors as SOFA score, age, dyspnea, and leukocytosis. The Area Under the Receiver-Operator Curve values for the risk factors in predicting critical illness were 0.921 for SOFA score, 0.776 for age, 0.764 for dyspnea, 0.658 for leukocytosis, and 0.960 for the combination of the four risk factors.ConclusionsOur findings advocate the use of risk factors SOFA score ≥2, age >60, dyspnea and leukocytosis >9.5 X109/L on admission, alone or in combination, to determine the optimal management of the patients and health care resources.
Comparative Effects Between High-intensity Atorvastatin and Rosuvastatin on New Onset Diabetes Mellitus and Cardiovascular Outcome
Authors: Choi Jah Yeon , Choi Byoung Geol , Park Yoonjee , Kang Dong Oh , Jang Won Young , Kim Woohyeun , Na Jin Oh , Kim Jin Won , Kim Eung Ju , Rha Seung-Woon , Park Chang Gyu , Seo Hong Seog , Jeong Myung Ho , Chae Sung-Chull , Seong In-Whan , Yoon Chang-Hwan , Cha Kwang Soo , Oh Seok Kyu , Choi Cheol Ung 
Published: June 2020

Abstract
Background: High-intensity statin is typically used in patients with acute myocardial infarction (AMI) for secondary prevention; however, concern regarding its association with diabetes mellitus has been consistent. We investigated and compared the impact of high-intensity atorvastatin on new-onset diabetes mellitus (NODM) and cardiovascular outcomes with that of high-intensity rosuvastatin for a 3-year follow-up period. Methods: Data from the Korea Acute Myocardial Infarction Registry were collected from November 2011 to October 2015, and 13,104 AMI patients were enrolled from major cardiovascular centers. Among them, 2,221 patients without diabetes who had been administered with high-intensity atorvastatin (40–80 mg) and rosuvastatin (20 mg) were investigated. The atorvastatin and rosuvastatin groups were compared and evaluated for the incidence of NODM and major adverse cardiac event (MACE) including total death, myocardial infarction, and revascularization cases in the following 3 years.Results: Baseline characteristics were comparable between two groups. Event-free survival rate of NODM and MACE was not significantly different between the atorvastatin and rosuvastatin groups (92.5% vs. 90.8%, respectively; Log-rank P-value = 0.550). The event-free survival rate of MACE was also not significantly different between atorvastatin and rosuvastatin groups (89.0% vs. 89.6%, respectively; Log rank p-value = 0.662). Multivariable Cox analysis revealed that the statin type was not a prognostic factor in the development of NODM and MACE. Conclusions: Administering high-intensity atorvastatin and rosuvastatin in AMI patients produced comparable effects on NODM and clinical outcome, suggesting their clinical equivalence in secondary prevention.
Habitually Late Bedtime is Associated With Elevated Serum Uric Acid and Monosodium Urate Monohydrate Deposition in Patients With Gout: A Case Control Study
Authors: Chen Xiaoting , Chen Lihui , Liu Fengjing , Chen Si , Han Ying , Hu Zhumeng , Chen Haibing 
Published: June 2020

Abstract
Background: In this study, we observed whether habitually late bedtime affects serum uric acid levels and monosodium urate monohydrate deposition in joints and tendons in patients with gout.Methods: This study included 195 patients with gout, who had been divided into two groups based on their bedtime (early or late) and compared serum uric acid levels between the groups. The study also compared ultrasounds of joints and tendons between groups, including the metatarsal-phalangeal joints, knee joints, and tendons of the lower limbs. A multivariate logistic regression was used to analyse the risk factors of monosodium urate monohydrate deposition.Results: Compared to patients with an early bedtime, those with a late bedtime had higher urate levels and a significantly higher proportion of double contour signs, tophi, and bone erosion in metatarsal-phalangeal joints (p < 0.05). Late-bedtime patients also had higher proportions of synovial hypertrophy and double contour signs in knee joints (p < 0.05), and a higher incidence of tophi in the quadriceps tendon (6.4%), compared to early-bedtime patients (2.5%; p < 0.05). Late bedtime was associated with monosodium urate monohydrate deposition. Conclusions: Patients with gout who had a late bedtime exhibited elevated urate levels, enhanced monosodium urate monohydrate deposition, and greater gout-related joint damage, compared to patients with an early bedtime.
Unimproved water, sanitation, and hygiene (WASH) and common childhood illness in Myanmar: evidence from a nationally representative survey
Authors: Naz Lubna , Ghimire Umesh 
Published: June 2020

Abstract
Background

Inadequate Water, Hygiene and Sanitation (WASH) is one of the major risk factors of common childhood illness - namely, diarrhea, cough, fever, and acute respiratory infection, in many Southeast Asian countries. This study aims to analyze the relationship between WASH-related indicators and common childhood illnesses; cough, fever and diarrhea among under-five children in Myanmar.
Methods

The data of 4,815 under-fives was extracted from the first Myanmar Demographic and Health Survey 2015-16. Chi-square test was used to determine association between WASH and sociodemographic variables and each common childhood illness. The bivariate logistic regression was used to obtain the unadjusted odds of cough, fever, and diarrhea for each wash indicator, i.e., Unimproved toilet, access to unsafe water, and unsafe feces disposal. Further multivariate regression was employed and adjusted odd ratios were obtained of each common childhood illness after accounting for all wash indicators.
Results

Among under-five children, 16.2% suffered from cough, 16% suffered from fever, and 10.4% from diarrhea. Unimproved toilet facility was associated with cough (Adjusted Odds Ratio (AOR) 1.15, 95% CI:0.86, 1.22), and fever (AOR 1.03, 95% CI:0.86,1.23) among children. Children from households practicing unimproved child feces disposal had 21%, 18%, and 52% higher odds of experiencing cough (95% CI:1.12, 1.31), fever (95% CI: 0.99,1.29), and diarrhea (95% CI:1.21, 1.68), respectively. A combination of unimproved water, toilet and child feces disposal facilities was associated with cough (AOR 1.34, 95% CI:1.03, 1.73), fever (AOR 1.12, 95% CI:0.86, 1.19) and diarrhea (AOR 1.18, 95% CI:0.95, 1.29).
Conclusion

Inadequate improved WASH significantly contributed to common childhood illnesses among children in Myanmar. Findings suggest that WASH interventions should be targeted to the poor and rural areas where the prevalence of both childhood illnesses and unimproved WASH facilities were reportedly high.
Long-term observation and sequencing analysis of SKPs-derived corneal endothelial cell-like cells for treating corneal endothelial dysfunction
Authors: Shen Lin , Sun Peng , Du Liqun , Zhu Jing , Ju Chengqun , Guo Hui , Pang Kunpeng , Wu Xinyi 
Published: June 2020

Abstract
Background
Corneal endothelial dysfunction is a principal cause of visual deficiency. Corneal transplantation is the most effective treatment for corneal endothelial dysfunction. However, a severe shortage of available donor corneas remains a global challenge. Previously, we acquired corneal endothelial cell-like cells (CEC-like cells) derived from skin-derived precursors (SKPs). CEC-like cells exerted excellent therapeutic effect in rabbit and monkey corneal endothelial dysfunction models.
Method:
We prolonged the observation in the monkey experiment for 2 years. Further transcriptome sequencing was also carried out on HCEC, CEC-like cells before transplantation and after transplantation.
Results
We confirmed that the monkey cornea remained transparent and the CEC-like cells could adapt to the microenvironment after transplantation. The gene expression pattern of CEC-like cells was similar to HCEC and changed slightly after transplantation.
Conclusions
This study presented a brand-new insight into CEC-like cells and further provided a promising prospect of cell-based therapy for corneal endothelial dysfunction. The renewable cell source, novel derivation method and simple treatment strategy may be clinically applied in regenerative medicine in the future.
Dynamic Changes of Lipid Profile in Severe Hypertriglyceridemia-Induced Acute Pancreatitis Patients Under Double Filtration Plasmapheresis: A Retrospective Observational Study
Authors: Zheng Huijun , Wang Donghai , Wang Xiaoling , Lin Yongjun , Lu Zhihua , Chen Yueliang , Feng Guo , Yang Na 
Published: June 2020

Abstract
Background: In this study, we aimed to investigate the dynamic changes of lipid profile under double filtration plasmapheresis (DFPP) in severe hypertriglyceridemia-induced acute pancreatitis (sHTGP) patients and ascertain whether these changes were associated with the prognosis of the disease.Methods: This retrospective study was conducted at a tertiary critical care center in China. We included sHTGP patients who were admitted within 72h after AP onset. All the sHTGP patients received DFPP within 24h after admission. Lipid profile changes was measured in 47 patients with sHTGP on admission, consecutive 4 days after DFPP and at discharge. According to the TG levels in the first day after DFPP, we divided patients into two group: non-target (TG>5.6mmol/L) and target group (TG ≤5.6mmol/L). Propensity score matching was used to investigate association between TG-lowering effect and the prognosis. Linear regression analysis on four models was used to determine the relationship between other lipid parameters and the outcome.Results: Except for low density lipoprotein, all the parameters of lipid profile at disease onset showed significant difference from their normal levels. The median serum triglyceride (TG) level of the sHTGP patients was 42.9 mmol/L on admission. In the first day after DFPP, serum TG, cholesterol (TC) and very low density lipoprotein decreased significantly, and the high-density lipoprotein (HDL) as well as apoprotein A1 increased meanwhile. TG continued to fall for the following three days and the other indicators held steady. After propensity score matching, the hospitalization duration was significantly lower in the target group (14.5 [13.0, 16.0] vs 8.0 [6.0, 15.3], P=0.035). Linear regression analysis revealed that the levels of HDL were negatively associated with the duration of hospitalization in three adjusted models.Conclusion: There was distinct fluctuation of the lipid profile upon the burst of sHTGP and the parameters changed significantly in the first day after DFPP. Among these parameters, HDL may serve as a biomarker for disease prognosis in patients with sHTGP. Moreover, reaching the TG-lowering target of 5.6mmol/L may shorten hospitalization duration.
Cancer stem cell marker-positive tumour nests in intrahepatic cholangiocarcinoma are related to epithelial type CD1a-positive dendritic cell infiltration and to high Sox9 expression
Authors: Utsunomiya Aya , Ohe Rintaro , Kabasawa Takanobu , Aung Naing Ye , Urano Yuka , Tamazawa Nobuyuki , Kitaoka Takumi , Suzuki Kazushi , Kawamura Ichiro , Kimura Wataru , Yamakawa Mitsunori 
Published: June 2020

Abstract
Background

It is yet a mystery whether dendritic cells (DCs) contact cancer stem cells (CSCs) and uptake CSC antigens in intrahepatic cholangiocarcinoma (ICC). The aim of this study was to examine the histological relationship between tumour cells expressing CSC markers and DCs infiltrating ICC. In addition, clinicopathological factors, including progression-free survival (PFS) and overall survival (OS), were compared between cases with many and few CSC marker-positive cells.
Materials and methods

Resected tissue sections of 22 cases with ICC were used for immunostaining to detect DC infiltration into ICC tumour nests positive for CD1a, DC-SIGN, DEC205, DC-LAMP, and CD123 and to detect ICC tumour cells positive for CSC markers such as CD44v9, EpCAM, and Sox9. The relationship between the number of CSC marker-positive cells and ICC clinicopathological factors was examined by Kaplan-Meier method and univariate and multivariate analyses using the Cox proportional hazards model.
Results

CD1a+ immature DCs infiltrated ICC tumour nests significantly more than the other types of DCs. Furthermore, they were frequently localized within CD44v9− and EpCAMhigh ICC tumour nests. According to Kaplan-Meier method, PFS and OS were longer in the Sox9high group than in the Sox9low group, and according to both univariate and multivariate analyses, Sox9 expression was an independent factor.
Conclusion

The present study first demonstrated that CD1a+ immature DCs frequently infiltrated CD44v9− and EpCAMhigh ICC tumour nests, suggesting the possibility of cell-to-cell contact between epithelial type immature DCs and CSCs in ICC. Furthermore, it was suggested that Sox9 expression in ICC may be a prognostic factor.
Novel Intelligent Method for Fault Diagnosis of Steam Turbine Based on T-SNE and XGBoost
Authors: Wang Xizhe , Zhang Lijun 
Published: June 2020

Abstract
For fault failures of a steam turbine occur frequently and cause huge losses, it is important to identify the fault category. A steam turbine clustering fault diagnosis method based on t-distribution stochastic neighborhood embedding (t-SNE) and extreme gradient boosting (XGBoost) is proposed. Firstly, the t-SNE algorithm is used to map high-dimensional data to low-dimensional space, and data clustering is performed in low-dimensional space. Combined with the fault records of the power plant, the fault data and health data of the clustering result are distinguished. Then, the imbalance problem in the data is processed by the synthetic minority over-sampling technique (SMOTE) algorithm to obtain the steam turbine characteristic data set with fault labels. Finally, we used the XGBoost to solve this multiclassification problem. In the experiment, the method achieved the best performance with an overall accuracy of 97% and early warning at least two hours in advance. The experimental results show that this method can effectively evaluate the state and make fault warning for power plant equipment.
Phenotypic Sexual Dimorphism in Response to Dietary Fat Manipulation in C57BL/6J Mice
Authors: Casimiro Isabel , Stull Natalie D. , Tersey Sarah A. , Mirmira Raghavendra 
Published: June 2020

Abstract
Background:Obesity and the metabolic syndrome are increasingly prevalent in society and their complications and response to treatment exhibit sexual dimorphism. Mouse models of high fat diet-induced obesity are commonly used for both mechanistic and therapeutic studies of metabolic disease and diabetes. However, the inclusion of female mammals in obesity research has not been a common practice, and has resulted in a paucity of data regarding the effect of sex on metabolic parameters and its applicability to humans. Methods:Here we analyzed male and female C57BL/6J mice beginning at 4 weeks of age that were placed on a low-fat diet (LFD, 10% calories from fat), a Western Diet (WD, 45% calories from fat), or a high fat diet (HFD, 60% calories from fat). Assessments of body composition, glucose homeostasis, insulin production, and energy metabolism, as well as histological analyses of pancreata were performed. Results:Both male and female C57BL/6J mice had similar increases in total percent body weight gain with both WD and HFD compared to LFD, however, male mice gained weight earlier upon HFD or WD feeding compared to female mice. Male mice exhibited a decrease in both food consumption and activity with either WD or HFD compared to LFD, whereas female mice did not exhibit any differences in food intake and minimal changes in locomotor activity on any diet. Glucose tolerance tests performed at 4, 12 and 20 weeks of dietary intervention revealed impaired glucose tolerance that was worse in male mice compared to females. Furthermore, male mice exhibited an increase in pancreatic β cell area as well as reduced insulin sensitivity after HFD feeding compared to WD or LFD, whereas female mice did not. Conclusions:Male and female C57BL/6J mice exhibited strikingly different responses in weight, food consumption, locomotor activity, and β cell adaptation upon dietary manipulation, with the latter exhibiting less striking phenotypic changes. We conclude that the nature of these responses emphasizes the need to contextualize studies of obesity pathophysiology and treatment with respect to sex.