bluuebunny/crossref_metadata_2025_split huggingface dataset:
Raman microspectroscopic study for the detection of oral field cancerisation using brush biopsy samples
Authors: Behl Isha , Calado Genecy , Vishwakarma Anika , Flint Stephen , Galvin Sheila , Healy Claire M. , Leite Pimentel Marina , Malkin Alison , Byrne Hugh J. , Lyng Fiona M. 
Published: June 2020

AbstractField cancerisation (FC) is potentially an underlying cause of poor treatment outcomes of oral squamous cell carcinoma (OSCC). To explore the phenomenon using Raman microspectroscopy, brush biopsies from the buccal mucosa, tongue, gingiva and alveolus of healthy donors (n = 40) and from potentially malignant lesions (PML) of Dysplasia Clinic patients (n = 40) were examined. Contralateral normal samples (n = 38) were also collected from the patients. Raman spectra were acquired from the nucleus and cytoplasm of each cell, and subjected to partial least squares‐discriminant analysis (PLS‐DA). High discriminatory accuracy for donor and PML samples was achieved for both cytopalmic and nuclear data sets. Notably, contralateral normal (patient) samples were also accurately discriminated from donor samples and contralateral normal samples from patients with multiple lesions showed a similar spectral profile to PML samples, strongly indicating a FC effect. These findings support the potential of Raman microspectroscopy as a screening tool for PML using oral exfoliated cells.
Interdisciplinary engineering education: A review of vision, teaching, and support
Authors: Van den Beemt Antoine , MacLeod Miles , Van der Veen Jan , Van de Ven Anne , van Baalen Sophie , Klaassen Renate , Boon Mieke 
Published: June 2020

AbstractBackgroundSocietal challenges that call for a new type of engineer suggest the need for the implementation of interdisciplinary engineering education (IEE). The aim of IEE is to train engineering students to bring together expertise from different disciplines in a single context. This review synthesizes IEE research with a focus on characterizing vision, teaching practices, and support.PurposeWe aim to show how IEE is conceptualized, implemented, and facilitated in higher engineering education at the levels of curricula and courses. This aim leads to two research questions:What aspects of vision, teaching, and support have emerged as topics of interest in empirical studies of IEE?What points of attention regarding vision, teaching, and support can be identified in empirical studies of IEE as supporting or challenging IEE?Scope/MethodNinety‐nine studies published between 2005 and 2016 were included in a qualitative analysis across studies. The procedure included formulation of research questions, searching and screening of studies according to inclusion/exclusion criteria, description of study characteristics, appraisal, and synthesis of results.ConclusionsChallenges exist for identifying clear learning goals and assessments for interdisciplinary education in engineering (vision). Most pedagogy for interdisciplinary learning is designed to promote collaborative teamwork requiring organization and team management. Our review suggests that developing interdisciplinary skills, knowledge, and values needs sound pedagogy and teaming experiences that provide students with authentic ways of engaging in interdisciplinary practice (teaching). Furthermore, there is a limited understanding of what resources hinder the development of engineering programs designed to support interdisciplinarity (support).
Genetic Counseling for Neurofibromatosis 1, Neurofibromatosis 2, and Schwannomatosis—Practice Resource of the National Society of Genetic Counselors
Authors: Radtke Heather B. , Bergner Amanda L. , Goetsch Allison L. , McGowan Caroline , Panzer Karin , Cannon Ashley 
Published: June 2020

AbstractThe goal of this practice resource is to provide genetic counselors and other healthcare professionals with a resource to reference when providing genetic counseling services to individuals and families undergoing evaluation for neurofibromatosis (NF) or who have received a diagnosis of NF, including NF1, NF2, and schwannomatosis. This resource represents the opinions of a multi‐center working group of Certified Genetic Counselors with experience in the care of individuals with NF, providing topics to be considered for the incorporation into a clinical genetic counseling session.
Surgical treatment for chronic pancreatitis: A single‐center retrospective study in Japan
Authors: Matsumoto Ippei , Kamei Keiko , Murase Takaaki , Yoshida Yuta , Kawaguchi Kohei , Matsumoto Masataka , Satoi Shumpei , Takebe Atsushi , Nakai Takuya , Takeyama Yoshifumi 
Published: June 2020

AbstractBackground/PurposeTo determine the short‐ and long‐term results of surgical treatments for chronic pancreatitis (CP) at a high‐volume center in Japan.MethodsThe records of 151 consecutive patients undergoing surgery for CP were retrospectively reviewed. Selection of surgical procedures used had been according to the Japanese Clinical Practice Guidelines for CP 2015. Long‐term (≥1 year) follow‐up was performed in 100 patients (median of 37 months).ResultsSurgical drainage procedures were performed in 107 patients (Frey operation in 81, longitudinal pancreaticojejunostomy in 26), pancreatic resection in 37 (subtotal stomach‐preserving pancreaticoduodenectomy in 11, distal pancreatectomy in 26), and other procedures in six. The rates of postoperative mortality and morbidity were 1% and 26%, respectively. The rates of complete and partial pain relief were 62% and 37%. The frequency of occurrence of severe morbidity was significantly higher after pancreatic resection than in patients receiving drainage procedures (13% vs 2%, P = .019). The rate of new‐onset diabetes was also significantly higher after resection than drainage (60% vs 25%, P = .017).ConclusionsSurgical treatment for painful chronic pancreatitis can be safe and effective. An optimal procedure should guarantee pain relief and preserve a maximum of pancreatic function.
New metastasectomy criteria for peritoneal metastasis of hepatocellular carcinoma: A study of the Japanese Society of Hepato‐Biliary‐Pancreatic Surgery
Authors: Iida Hiroya , Tani Masaji , Aihara Tsukasa , Hasegawa Kiyoshi , Eguchi Hidetoshi , Tanabe Minoru , Yamamoto Masakazu , Yamaue Hiroki 
Published: June 2020

AbstractBackground/PurposePeritoneal metastasis of hepatocellular carcinoma (HCC) is rare. We investigated patients who underwent peritoneal metastasectomy in a multicenter study in Japan.MethodsThe study included 92 patients with HCC who underwent resection for peritoneal metastasis between January 2007 and December 2013. We investigated background and operative factors, as well as overall and recurrence‐free survival rates. Patients were classified according to the extent of peritoneal metastasis using the peritoneal cancer index (PCI) and the completeness of cytoreduction (CC) scores to examine whether peritoneal metastasectomy contributed to survival.ResultsThe mean maximum tumor size was 4.1 cm. Forty patients (43.5%) had multiple peritoneal metastases. Peritoneal metastasectomy and hepatectomy were performed simultaneously in 48 patients (52.2%). Overall, the 5‐year survival rate after resection was 36.0%, and the recurrence‐free survival rate was 13.0%. Multivariate analysis revealed that PCI scores of ≤6 + CC scores of 0 were an independent prognostic factor. The 5‐year survival rate when these criteria were met was 43%, which was significantly better than that in those who did not meet these criteria (P < .001).ConclusionsPeritoneal metastasectomy of HCC appears to contribute to improved survival of patients with a PCI scores of ≤6, without remnant tumors.
Access to HIV prevention services in East African cross‐border areas: a 2016‐2017 cross‐sectional bio‐behavioural study
Authors: Virkud Arti V , Arimi Peter , Ssengooba Freddie , Mulholland Grace E , Herce Michael E , Markiewicz Milissa , Weir Sharon , Edwards Jessie K 
Published: June 2020

AbstractIntroductionEast African cross‐border areas are visited by mobile and vulnerable populations, such as men, female sex workers, men who have sex with men, truck drivers, fisher folks and young women. These groups may not benefit from traditional HIV prevention interventions available at the health facilities where they live, but may benefit from services offered at public venues identified as places where people meet new sexual partners (e.g. bars, nightclubs, transportation hubs and guest houses). The goal of this analysis was to estimate availability, access and uptake of prevention services by populations who visit these venues.MethodsWe collected cross‐sectional data using the Priorities for Local AIDS Control Efforts sampling method at cross‐border locations near or along the land and lake borders of Kenya, Rwanda, Tanzania and Uganda from June 2016–February 2017. This bio‐behavioural survey captured information from a probability sample of 11,428 individuals at 833 venues across all areas. Data were weighted using survey sampling weights and analysed using methods to account for the complex sampling design.ResultsAmong the 85.6% of persons who had access to condoms, 60.5% did not use a condom at their last anal or vaginal sexual encounter. Venues visited by high percentages of persons living with HIV were not more likely than other venues to offer condoms. In 12 of the 22 cross‐border areas, male or female condoms were available at less than 33% of the venues visited by persons having difficulty accessing condoms. In 17 of the 22 cross‐border areas, education outreach visits in the preceding six months occurred at less than 50% of the venues where participants had low effective use of condoms.ConclusionsIndividuals visiting venues in cross‐border areas report poor access to and low effective use of condoms and other prevention services. Availability of HIV prevention services differed by venue and population type and cross‐border area, suggesting opportunities for more granular targeting of HIV prevention interventions and transnational coordination of HIV programming.
Disparities in the PrEP continuum for trans women compared to MSM in San Francisco, California: results from population‐based cross‐sectional behavioural surveillance studies
Authors: Wilson Erin C , Turner Caitlin M , Arayasirikul Sean , Lightfoot Marguerita , Scheer Susan , Raymond Henry F , Liu Albert 
Published: June 2020

AbstractIntroductionAlthough transgender women (trans women) often are conflated with men who have sex with men (MSM) in HIV research and services, there are distinct population differences that are important for implementing effective HIV prevention. Our objective was to examine pre‐exposure prophylaxis (PrEP) disparities between the two populations and compare individual, social and structural factors that influence differences between MSM and trans women along the PrEP continuum.MethodsWe analysed data from two population‐based studies, one with trans women (Trans*National Study, 2016 ‐ 18) and the other with MSM (National HIV Behavioral Surveillance, 2017). Trans women were recruited via respondent‐driven sampling and MSM using time location sampling. Key indicators of the PrEP continuum were evaluated, including awareness, health insurance, provider discussions, recent use and adherence. Associations were also examined for PrEP continuum indicators and structural barriers (e.g. employment, homelessness).ResultsTranswomen were more likely than MSM to be Latino/a (30.4% vs. 25.8%; prevalence ratio (PR)=1.08, 95% CI 1.02 to 1.14) or African American (7.1% vs. 4.5%; PR = 1.12, 1.02 to 1.24), live at or below the poverty limit (70.7% vs. 15.8%; PR = 1.47; 1.41 to 1.53), be unemployed (50.1% vs. 26.3%; PR = 1.18, 1.13 to 1.24), be homeless (8.4% vs. 3.5%; PR = 1.15, 1.06 to 1.25) and to have less than a college degree (PR = 1.41, 1.34 to 1.48). Trans women were more likely than MSM to have health insurance (95.7% vs. 89.7%, PR = 1.17, 1.06 to 1.28), but less likely than MSM to have heard of PrEP (79.1% vs. 96.7%; PR = 0.77, 0.73 to 0.81), talked with a provider about PrEP (35.5% vs. 54.9%; PR = 0.87, 0.83 to 0.91) and less likely than MSM to have used PrEP in the past six months (14.6% vs. 39.8%; PR = 0.80, 0.76 to 0.84). Among PrEP users, trans women were less likely to report being adherent to PrEP than MSM (70.4% vs. 87.4%; PR = 0.80, 0.70 to 0.91).ConclusionsWe found PrEP disparities for trans women compared to MSM and the need for differentiated implementation strategies to meet the specific PrEP barriers trans women face. Inclusion of trans women’s HIV risks is needed in CDC guidance for PrEP. Interventions to increase trans women’s awareness of PrEP including at the provider and community level are also needed. Finally, programming that addresses trans women’s barriers to housing and income is also needed to reduce PrEP disparities.
Matrix metalloproteinase 3 as a valuable marker for patients with COVID‐19
Authors: Shi Shengjie , Su Min , Shen Ge , Hu Yan , Yi Fan , Zeng Ziyan , Zhu Pan , Yang Gang , Zhou Hui , Li Qiong , Xie Xiaobing 
Published: June 2020

AbstractThe situation of the coronavirus disease 2019 (COVID‐19) continues to evolve, our study explored the significance of serum levels of matrix metalloproteinase 3 (MMP3) as a marker for patients with COVID‐19. Sixty‐two COVID‐19 patients in the First Hospital of Hunan University of Chinese Medicine and Loudi Center for Diseases Prevention and Control, from January to March 2020, were sampled as the novel coronavirus pneumonia infected group. One hundred and thirty‐one cases from the First Hospital of Hunan University of Chinese Medicine, including 67 healthy individuals and 64 non‐COVID‐19 inpatients, served as the noninfected group. Approximately every 5 days, sera from 20 cases were collected and analyzed three times, using an automatic biochemical analyzer, to detect serum MMP3 concentrations. Correlation was analyzed between MMP3 and other proinflammatory cytokines. Following normality tests, differences in serum MMP3 levels between the infected and noninfected group were analyzed via SPSS (version 25.0) software, using the Wilcoxon rank sum test. The MMP3 concentration was 44.44 (23.46 ~ 72.12) ng/mL in the infected group and 32.42 (28.16 ~ 41.21) ng/mL in the noninfected group. The difference between the two groups was statistically significant (Z = −2.799, P = .005 < .05). A positive correlation was found between MMP3 and interleukin 1β (IL‐1β; r = .681, P = .000 < .05), and IL‐6 (r = .529, P = .002 < .05). Serum MMP3 concentration, measured over three separate time points, were 55.98 (30.80 ~ 75.97) ng/mL, 34.84 (0.00 ~ 51.84) ng/mL, and 5.71 (0.00 ~ 40.46) ng/mL, respectively. Detection of serum MMP3 levels may play an important role in the development of therapeutic approaches for COVID‐19 and may indicate the severity of disease.
Association between a progesterone receptor mutation and hepatitis E sero‐positivity in liver transplant recipients
Authors: Debes Jose D. , Groothuismink Zwier M. A. , de Man Robert A. , Boonstra Andre 
Published: June 2020

AbstractProblemWe investigated if the PROGINS mutation increases the risk of hepatitis E virus (HEV) infection in liver transplant recipients. PROGINS was analyzed through KASP assay; HEV serologies assessed via enzyme‐linked immunosorbent assay and multiplex cytokine assays were evaluated in plasma with the ProcartaPlex human immunoassay. Seventy liver transplant recipients were evaluated, of which 23 (33%) were HEV immunoglobuln G (IgG)‐positive (HEV+). The frequency of PROGINS in the HEV+ group was 34%, compared with 14% in those that were HEV IgG negative (HEV−). Cytokine measurements in a sub‐set of samples from HEV+/PROGINS+ individuals showed decreased plasma levels of monokine induced by gamma interferon, a proliferation‐inducing ligand, and stem cell factor, as well as increased levels of eotaxin‐3 and interleukin‐31 compared with those HEV−/PROGINS− samples. Our findings suggest an association between the PROGINS mutation and seropositivity for HEV in liver transplant recipients with consequent distorted cytokine levels.
Intratonsillar detection of 27 distinct viruses: A cross‐sectional study
Authors: Silvoniemi Antti , Mikola Emilia , Ivaska Lotta , Jeskanen Marja , Löyttyniemi Eliisa , Puhakka Tuomo , Vuorinen Tytti , Jartti Tuomas 
Published: June 2020

AbstractPalatine tonsils have been observed to harbor several distinct respiratory and herpesviruses in separate studies. In this study, the presence of these viruses in palatine tonsils was comprehensively studied in both children and adults. A cross‐sectional analysis of 181 patients (median age 22 years; range, 2.6‐66) operated for a benign tonsillar disease was conducted. Real‐time polymerase chain reaction was performed to detect 27 distinct viruses in all: eight human herpesviruses, 16 respiratory viruses, parvo B19, and polyoma BK/JC viruses. Clinical characteristics of the patients and underlying conditions were evaluated. In total, 92% of patients had virus detected in tonsils (Epstein‐Barr virus 72%, human herpesvirus 7, and 6B 54% and 16%, respectively, enterovirus 18%, parvovirus B19 7% and the rest <4%). No herpes simplex virus 2, varicella zoster virus, polyoma JC virus, parainfluenza‐, metapneumo‐, or coronaviruses were found. Enterovirus was more common in children and was frequently observed in the presence of HHV6B. None of the viruses showed a positive association to the tonsillar disease. Respiratory symptoms were not associated with the prevalence of viruses. This study comprehensively reports a cross‐sectional view of intratonsillar virus infections in elective tonsillectomy patients in a wide age range cohort. Tonsils are a major virus reservoir for distinct herpes and respiratory viruses without a positive association with tonsillar disease or respiratory symptoms.
Study of the azo‐hydrazone tautomerism of Acid Orange 20 by spectroscopic techniques: UV–Visible, Raman, and surface‐enhanced Raman scattering
Authors: Vannucci Giulia , Cañamares Maria Vega , Prati Silvia , Sanchez‐Cortes Santiago 
Published: June 2020

AbstractAcid Organge 20 is a synthetic monoazo dye, which molecular structure consists of a naphtol unit and a sulfonated benezene ring linked together by an azo chromophore group. As a phenylazonaphthol with the OH group in para position respect to the azo group, it undergoes an azo‐hydrazone tautomerism. In this work, the study of the preponderance of one tautomer over the other in different pH conditions was carried out by means of UV–Vis, Raman, and surface‐enhanced Raman scattering (SERS) spectroscopies. Raman spectra of powder and aqueous solutions of the dye were obtained at different excitation lines at the natural pH of the AO20 solutions. The best results were obtained at 633 nm, and the observed Raman bands suggested the presence of the hydrazone tautomer. The molecular structure and the theoretical Raman spectra of the two tautomers were calculated by density functional theory (DFT) methods. The obtained results were used for the assignment of the Acid Orange 20 vibrational modes. The SERS analysis of the dye solutions were carried out employing Ag nanostars, pH below and above 4.7, and excitation at 442, 532, and 633 nm. The hydrazone tautomer was prevalent in most conditions. The azo tautomer was only detected at pH ~ 5 under excitation with the 532 nm wavelength due to resonant Raman conditions. Finally, the effect of pH on the morphology of metal nanoparticles employed as SERS substrates was evaluated by TEM.
Recurrence beyond the Milan criteria after curative‐intent resection of hepatocellular carcinoma: A novel tumor‐burden based prediction model
Authors: Tsilimigras Diamantis I. , Mehta Rittal , Guglielmi Alfredo , Ratti Francesca , Marques Hugo P. , Soubrane Olivier , Lam Vincent , Poultsides George A. , Popescu Irinel , Alexandrescu Sorin , Martel Guillaume , Hugh Tom , Aldrighetti Luca , Endo Itaru , Pawlik Timothy M. 
Published: June 2020

AbstractBackgroundAccurate prediction of recurrence patterns of hepatocellular carcinoma (HCC) may allow for prioritization of patients for resection or transplantation as well as guide post‐resection surveillance strategies.MethodsPatients who underwent curative‐intent R0 resection for HCC between 2000 and 2017 were identified using a multi‐institutional database. A prognostic model that incorporated HCC tumor burden score (TBS) to predict recurrence beyond the Milan criteria (MC) was developed and validated.ResultsAmong 718 patients who underwent R0 resection for HCC, 185 (25.8%) recurred within and 110 (15.3%) beyond the MC. On multivariable analysis, AFP more than 400 ng/mL (hazard ratio [HR] = 2.26; 95% confidence interval [CI]: 1.27‐4.02), lymphovascular invasion (HR = 2.00; 95% CI: 1.14‐3.50), and TBS (HR = 1.08; 95% CI: 1.03‐1.12) were associated with recurrence beyond the MC. A weighted TBS‐based score was constructed: [0.074*TBS + 0.692*lymphovascular invasion (yes: 1, no: 0) + 0.816*AFP > 400 (yes:1, no:0)]. Patients with a low, medium, and high TBS‐based risk score had a 5‐year incidence of recurring beyond the MC of 16.2%, 28.6%, and 47.2%, respectively (P < .001). The predictive accuracy of the model was very good in the training (C‐index: 0.761) and validation (C‐index: 0.706) datasets and outperformed the previously reported clinical risk score (CRS; C‐index: 0.680).ConclusionA TBS‐based model accurately predicted recurrence beyond MC after curative‐intent resection of HCC and outperformed the CRS. Incorporating TBS allows for better risk stratification and identifies patients in need of closer surveillance.
Challenges in sentinel node pathology in the era of adjuvant treatment
Authors: Franke Viola , Madu Max F. , Bierman Carolien , Klop Willem M. C. , van Houdt Winan J. , Wouters Michel W. J. M. , van de Wiel Bart A. , van Akkooi Alexander C. J. 
Published: June 2020

AbstractBackgroundWith the approval of adjuvant therapy for stage III melanoma, accurate staging is more important than ever. Sentinel node biopsy (SNB) is an accurate staging tool, yet the presence of capsular nevi (CN) can lead to a false‐positive diagnosis.Patients and MethodsRetrospective analysis of the American Joint Committee on Cancer 7th edition stage IIIA melanoma patients who were treated at our institute between 2000 and 2015. SNB slides were reviewed for this study by an expert melanoma pathologist.ResultsOf 159 eligible patients, 14 originally diagnosed with metastatic melanoma merely had CN (8.8%). Another two merely had melanophages (1.3%). Thus, 10.1% of SNs were considered false positive after revision. In 12 patients, the SN tumor burden was originally reported as larger than 1 mm but turned out to be less than 1 mm. Four patients originally reported as SN tumor burden less than 1 mm before revision turned out to have larger than 1 mm. These patients might have been over‐ or undertreated in the current era of adjuvant therapy for stage III melanoma.ConclusionsDistinguishing metastatic melanoma from benign CN and melanophages can be a diagnostic challenge. We plead for an expert pathologists' review, especially when using the SNB + results to determine treatment consequences.