bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alternative to xylene as a clearing agent in histopathology
Authors: Alwahaibi Nasar , Aljaradi Shaima , Alazri Horiyah 
Published: February 2020

ABSTRACT
INTRODUCTION: Clearing is an essential step in processing tissue for light microscopy. Xylene is the clearing agent used most commonly worldwide. Xylene is toxic and therefore a threat to personnel working in histopathology laboratories. We evaluated a safer alternative clearing agent for use in the histopathology laboratory.
MATERIALS AND METHODS: We used 230 formalin-fixed, paraffin-embedded tissue blocks from 19 different tissues. Half of the specimens were processed using xylene and half were processed using UltraClear™. Tissues were evaluated for eight parameters: sectioning, nuclear staining, cytoplasmic staining, overall cell morphology, clarity of staining, uniformity of staining, quality of immunohistochemistry (IHC), and cost.
RESULTS: Both UltraClear™ and xylene processed sections scored 100% for IHC. Sections processed using UltraClear™ were easy to cut (81.7%) as were xylene processed sections (96.5%). UltraClear™ processed sections showed 67%, 60.9%, 52.2%, 63.5%, and 67% for nuclear staining, cytoplasmic staining, cell morphology, clarity of staining, and uniformity of staining, respectively. UltraClear™ is twice as expensive as xylene. We found that tissues processed using UltraClear™ were easy to cut and worked well for both hematoxylin and eosin and IHC staining.
CONCLUSION: UltraClear™ is less toxic, less flammable, friendlier to the environment, and easy to handle, but it is two times expensive than xylene. The findings of this study recommend the use of UltraClear™ solution as a routine clearing agent in histopathology laboratories. However, further studies are required.
Hemolysis area: A new parameter of erythrocyte osmotic fragility for screening of thalassemia trait
Authors: Tatu Thanusak , Sweatman Denis 
Published: February 2020

Abstract
BACKGROUND: One-tube osmotic fragility test (OFT) is widely used for screening thalassemia traits. Interobserver variation may occur with 0.36% NaCl-based OFT due to the naked eye result reading style.
PURPOSE: The purpose of this study was to establish and evaluate the novel numerical OFT-based parameter, so-called hemolysis area (HA), in screening thalassemia traits.
MATERIALS AND METHODS: The portable spectrophotometer was invented capable of calculating the HA values. The HA values were then compared among 69, 156, and 19 blood samples having positive, negative, and suspicious 0.36% NaCl-based OFT results, respectively; 109 and 135 blood samples having mean corpuscular volume (MCV) ≤80 fL and >80 fL, respectively; and 138 and 106 blood samples having mean corpuscular hemoglobin (MCH) ≤27 pg and >27 pg, respectively. In addition, the HA values were compared in 166 blood samples having different globin gene genotypes. Finally, the HA cutoff value was determined by receiver operation curve (ROC) analysis.
RESULTS: The HA values in samples having positive, suspicious, and negative 0.36% NaCl-based OFT were 33.3 ± 14.4, 42.9 ± 10.5, and 65.3 ± 13.4, respectively; in sample having MCV ≤80 fL and >80 fL were 43.1 ± 19.6 and 63.8 ± 14.5, respectively; and in samples having MCH ≤27 pg and >27 pg were 46.7 ± 20.1 and 64.8 ± 14.2, respectively. The HA values in normal, hemoglobin E, SEA-a thalassemia 1, and β-thalassemia traits were 67.1 ± 12.6, 36.4 ± 13.9, 20.2 ± 4.8, and 18.6 ± 1.1, respectively. All were significantly different. ROC analysis established 52.4 as the HA cutoff that had comparable effectiveness to the conventional screening tests.
CONCLUSION: The new HA value was effective and could be an alternative choice for screening thalassemia traits.
Malignant melanoma of conjunctiva: Diagnosis on fine-needle aspiration cytology
Authors: Kishore Manjari , Kumar Vijay , Kaushal Manju 
Published: February 2020

ABSTRACTMalignant melanoma of conjunctiva is an uncommon malignancy. The diagnosis is suspected clinically and confirmed on pathological examination. We present a case of a 63-year-old female with previous history of pigmented lesion in the right eye and subsequently diagnosed as malignant melanoma of right conjunctiva and cornea on fine-needle aspiration cytology and histopathology.
Cytodiagnosis of Rosai–Dorfman disease masquerading as lymphoma: A case report with brief review of literature
Authors: Kishore Manjari , Gupta Prajwala , Ahuja Arvind , Bhardwaj Minakshi 
Published: February 2020

ABSTRACTRosai–Dorfman disease (RDD) or sinus histiocytosis with massive lymphadenopathy (SHML) is a self-limiting, benign histiocytic disorder. We report a case of RDD mimicking lymphoma clinically and cytologically in a 3-year-old male child. The present case highlights the importance of cytology in the diagnosis of RDD along with cytological features of other differential diagnoses.
Prevalence and types of preanalytical error in hematology laboratory of a tertiary care hospital in South India
Authors: Arul Pitchaikaran , Pushparaj Magesh , Pandian Kanmani , Chennimalai Lingasamy , Rajendran Karthika , Selvaraj Eniya , Masilamani Suresh 
Published: February 2020

ABSTRACT
BACKGROUND: An important component of laboratory medicine is preanalytical phase. Since laboratory report plays a major role in patient management, more importance should be given to the quality of laboratory tests.
AIM: The present study was undertaken to find the prevalence and types of preanalytical errors at a tertiary care hospital in South India.
MATERIALS AND METHODS: In this cross-sectional study, a total of 118,732 samples ([62,474 outpatient department [OPD] and 56,258 inpatient department [IPD]) were received in hematology laboratory. These samples were analyzed for preanalytical errors such as misidentification, incorrect vials, inadequate samples, clotted samples, diluted samples, and hemolyzed samples.
RESULTS: The overall prevalence of preanalytical errors found was 513 samples, which is 0.43% of the total number of samples received. The most common preanalytical error observed was inadequate samples followed by clotted samples. Overall frequencies (both OPD and IPD) of preanalytical errors such as misidentification, incorrect vials, inadequate samples, clotted samples, diluted samples, and hemolyzed samples were 0.02%, 0.05%, 0.2%, 0.12%, 0.02%, and 0.03%, respectively.
CONCLUSION: The present study concluded that incorrect phlebotomy techniques due to lack of awareness is the main reason for preanalytical errors. This can be avoided by proper communication and coordination between laboratory and wards, proper training and continuing medical education programs for laboratory and paramedical staffs, and knowledge of the intervening factors that can influence laboratory results.
An Application of Spherical Geometry to Hyperkähler Slices
Authors: Crooks Peter , van Pruijssen Maarten 
Published: February 2020

AbstractThis work is concerned with Bielawski’s hyperkähler slices in the cotangent bundles of homogeneous affine varieties. One can associate such a slice with the data of a complex semisimple Lie group $G$, a reductive subgroup $H\subseteq G$, and a Slodowy slice $S\subseteq \mathfrak{g}:=\text{Lie}(G)$, defining it to be the hyperkähler quotient of $T^{\ast }(G/H)\times (G\times S)$ by a maximal compact subgroup of $G$. This hyperkähler slice is empty in some of the most elementary cases (e.g., when $S$ is regular and $(G,H)=(\text{SL}_{n+1},\text{GL}_{n})$, $n\geqslant 3$), prompting us to seek necessary and sufficient conditions for non-emptiness.We give a spherical-geometric characterization of the non-empty hyperkähler slices that arise when $S=S_{\text{reg}}$ is a regular Slodowy slice, proving that non-emptiness is equivalent to the so-called $\mathfrak{a}$-regularity of $(G,H)$. This $\mathfrak{a}$-regularity condition is formulated in several equivalent ways, one being a concrete condition on the rank and complexity of $G/H$. We also provide a classification of the $\mathfrak{a}$-regular pairs $(G,H)$ in which $H$ is a reductive spherical subgroup. Our arguments make essential use of Knop’s results on moment map images and Losev’s algorithm for computing Cartan spaces.
SNC Log Symplectic Structures on Fano Products
Authors: Okumura Katsuhiko 
Published: February 2020

AbstractThis paper classifies Poisson structures with the reduced simple normal crossing divisor on a product of Fano varieties of Picard number 1. The characterization of even-dimensional projective spaces from the viewpoint of Poisson structures is given by Lima and Pereira. In this paper, we generalize the characterization of projective spaces to any dimension.
Assessment of patient satisfaction of certified and non-certified private dental clinics in Athens
Authors: Zampa Evangelia , Silvestros Spyridon , Kapaki Vasiliki , Souliotis Kyriakos 
Published: February 2020

Background: In recent years, the issue of quality assurance in health services has emerged in the forefront, with strong research and practical interest. In addition, there is a number of changes to health service users who nowadays have higher expectations of satisfaction, efficiency and quality of health services.Aim: The aim of the present study is to assess the satisfaction of patients and, by extension, the quality of the services provided both in certified and non-certified private dental practices in Athens, to highlight the difference in the services provided and the areas in which it appears.Methods: This is a quantitative study of dental patients’ satisfaction in Athens using an anonymous and self-completed questionnaire. The aggregate sample collected is 317 patients within three months of the questionnaire being shared. In particular, the sample for certified dental places was 176 patients, while the sample of non-certified dental places was 141 patients. For the statistical analysis, the SPSS program for Windows version 25 was used, and the tables were created with Microsoft Excel 2010 based on the SPSS analysis data.Results: The results showed differences between the social level of patients, the permanent residence of the participants as well as differences in the reason for choice and visit to each dental place, while on the questions about the satisfaction of the services the differences were little. The questions with the most shared responses were those in the financial basis. Correlation coefficients did not show any statistically significant relationship between demographic characteristics and satisfaction, with the exception of the level of education of patients in certified dental clinics.Conclusions: Although the results of the research showed little differences in the satisfaction of the patients between certified and non-certified dental clinics, these results are quite limited due to the small number of certified dental clinics in Greece. Further research is needed to produce safer conclusions.
Produksi Metil 12-Hidroksistearat via Proses Hidrogenasi Ester Minyak Jarak
Authors: Pasaribu Indra G. , Rizky Arry K. , Gultom Mario C. , Dewi Retno G. , Prakoso Tirto P. , Sitompul Johnner P. 
Published: February 2020

bstrak. Minyak jarak kaliki (Ricinus communis L.) merupakan salah satu bahan baku terbarukan yang banyak diaplikasikan di berbagai industri kimia. Salah satu dari turunan minyak jarak kaliki yaitu metil 12-hidroksistearat merupakan metil ester yang memiliki manfaat dalam berbagai jenis industri, khususnya industri pelumas. Makalah ini membahas produksi metil 12-hidroksistearat via hidrogenasi ester minyak jarak dalam reaktor mini-pilot. Makalah ini membahas kondisi optimum proses hidrogenasi dari metil ester risinoleat dengan melakukan variasi terhadap tekanan dan temperatur operasi. Tekanan dan temperatur operasi proses hidrogenasi divariasikan pada rentang 2-4 bar dan 150-210℃. Diperoleh produk hidrogenasi metil ester risinoleat dengan konsentrasi massa metil 12-hidroksistearat mencapai 61,28% dan angka iodin 23,84. Peningkatan temperatur dan tekanan operasi meningkatkan konversi metil 12-hidroksistearat dan menurunkan angka iodin. Pada rentang variasi kondisi, kondisi operasi optimum untuk proses ini adalah temperatur 210℃ dan tekanan 4 bar. Penambahan waktu reaksi hidrogenasi selama 3 jam menghasilkan penurunan angka iodin hingga mencapai 9,82 dan bentuk fisik seperti mentega. Kata kunci: angka iodin, hidrogenasi, metil 12-hidroksistearat, metil ester risinoleat, minyak jarak kaliki. Abstract. Production of Methyl 12-Hydroxystearate through the Hydrogenation Process of Castor Oil Esters. Castor oil (Ricinus communis L.) is one of renewable raw materials widely applied in various chemical industries. One of the castor oil derivatives is methyl 12-hydroxistearate, a methyl ester fatty acid which has been applied in various industries especially lubricants industry. This paper discusses the methyl ester production of methyl 12-hydroxystearate from castor oil via hydrogenation of ester of castor oil in a mini-pilot reactor. The purpose of this study is to determine the optimum operating condition of the hydrogenation process of the ricinoleic acid methyl ester by varying operating pressure and temperature. The operating pressure and temperature of the hydrogenation process were varied in the range of 2-4 bar and 150-210℃. The experimental results show that the hydrogenation process of ricinoleic acid methyl ester produces methyl-12-hydroxistearate with concentration up to 61.28%-wt and Iodine Value of 23.84. Higher operating temperature and pressure increase the conversion of methyl 12- hydroxistearate and reduce the iodine value. Further, the optimum operation condition for this process is at 210℃ and 4 bar. Increasing reaction time for about 3 hours results in a significant reduction of iodine value up to 9.82. Keywords: castor oil, hydrogenation, iodine number, methyl 12-hidroxystearate, methyl ester ricinoleic. Graphical Abstract
Skolegudstjenester. Føringer, friksjon, forandring
Authors: Øierud Gunnfrid Ljones 
Published: February 2020

Skolegudstjenester er en utbredt praksis i Norge. Ettersom skole og kirke framstår som stadig mer klart atskilte og ulike institusjoner eller praksisfelt, er det et spørsmål om skolegudstjenester kan gjennomføres i tråd med føringer fra begge hold? Artikkelen presenterer og drøfter praksisføringer for skolegudstjenester fra henholdvis skole og kirke med vekt på kirkelige føringer, diskuterer kompatibilitet og vurderer mulighetene for å gjennomføre skolegudstjenester på en måte som hensyntar de ulike føringene. Diskusjonsområdene er begrunnelser for skolegudstjeneste, gudstjenesteutforming og elevdeltakelse, med mest vekt på deltakelse. De analytiske funnene viser både kompatibilitet og friksjon, og funnene drøftes opp mot eksisterende variasjoner i praksis samt religionsdidaktiske, liturgiske og ritualteoretiske perspektiver.Nøkkelord: skolegudstjeneste, deltakelse, elevinvolvering, fritak, gudstjeneste, samarbeid skole–kirkeWorship services for public school students are common in Norway. As church and school have become more clearly different and unrelated institutions or fields of practice – is it possible to enact worship services in accordance with regulations from both institutions? This article presents and analyses school worship relevant practice regulations from both church and school, with most emphasis on church. It discusses regulation compatibility and practice possibilities, focusing on three topics: reasons for school worship, worship design, and most extensively: student participation. The analytical findings of both compatibility and friction are discussed in relation to pedagogical, liturgical and ritual theoretical perspectives. Keywords: school worship service, student participation, exemption, worship, liturgy, church and school collaboration
Le commun contre l’État-nation ?
Authors: Vibert Stéphane , Martin Éric 
Published: February 2020

La proposition d’un « commun » multidimensionnel, qui comprend à la fois des pratiques collectives, des décisions politiques et des réflexions théoriques, souhaite dégager une « troisième voie », à distance tant du marché que de l’État, qui, dans la perspective de ses promoteurs, permettrait de restaurer une démocratie radicale, synonyme d’autogouvernement et d’extension des droits d’usage collectif. Cette contribution vise à remettre en question les soubassements théoriques et la viabilité pratique de cette « sociopolitique du commun » (proposée notamment dans l’ouvrage de Dardot et Laval, Commun. Essai sur la révolution au XXIe siècle), qui érige l’auto-institution en dispositif universel de la praxis associative afin de contourner les difficultés à assurer un « service public » sans recourir aux mécanismes institutionnels d’un État perçu comme vecteur de domination centralisée. À trop insister sur le « faire société » pragmatique au détriment de tout « être institué » considéré essentialiste, cette anthropologie philosophique encourt le risque de manquer le « bien commun » imaginaire et symbolique inhérent à toute communauté concrète.
Daily Integrated Care Conferences to Reduce Length of Hospital Stay for Patients With Chronic Obstructive Pulmonary Disease
Authors: Shilian Ryan , Abraham Tina , Wynbrandt Jonathan , Jhaveri Devi , Hostoffer Robert W. , Peppers Brian P. 
Published: February 2020

Abstract

Context
Inefficiencies in care coordination—specifically, the lack of an effective method of communication among multiple health care professionals—often leads to an unnecessary increase in length of hospital stay.

Objective
To determine whether daily integrated care conferences (ICCs) would significantly reduce the length of stay for patients with chronic obstructive pulmonary disease (COPD) exacerbation.

Method
Patients with COPD exacerbation were selected for the study using electronic medical records from 2 osteopathic community hospitals located in northeastern Ohio. One hospital used daily ICCs and the other hospital did not use daily ICCs. The average length of stay for patients at each hospital was retrospectively investigated.

Results
A total of 1683 patients with COPD exacerbation were selected. The mean (SD) length of stay in the hospital with daily ICCs was 3.37 (2.89) days compared with 5.55 (3.99) days in the hospital without daily ICCs (P<.0001). At the hospital with daily ICCs, patients aged 40 to 69 years had a 67% shorter hospital stay and patients aged 70 to 99 years or older had a 36% shorter length of stay compared with patients at the hospital without daily ICCs.

Conclusion
Daily integrated care conferences significantly reduced the length of stay for patients with COPD exacerbation at an osteopathic community-based hospital. Implementing daily ICCs may make current health care services and coordinated care more efficient, resulting in decreased costs and length of stay for patients with COPD exacerbation.
Sound production in the Meagre,Argyrosomus regius(Asso, 1801): intraspecific variability associated with size, sex and context
Authors: Pereira Beatriz P. , Vieira Manuel , Pousão-Ferreira Pedro , Candeias-Mendes Ana , Barata Marisa , Fonseca Paulo J. , Amorim Maria Clara P. 
Published: February 2020

BackgroundMany fish taxa produce sound in voluntary and in disturbance contexts but information on the full acoustic repertoire is lacking for most species. Yet, this knowledge is critical to enable monitoring fish populations in nature through acoustic monitoring.MethodsIn this study we characterized the sounds emitted during disturbance and voluntary contexts by juvenile and adult meagre,Argyrosomus regius, in laboratory conditions. Breeding sounds produced by captive adults were also compared with meagre sounds registered in the Tagus estuary (Lisbon, Portugal) from unseen fish during the breeding season.ResultsThe present dataset demonstrates for the first time that in this species dominant frequency is inversely related to fish size, and that sounds vary according to sex, context and age. Sounds from captive breeding adults were similar to sounds recorded in the field.DiscussionOur findings indicate thatA. regiussound features carry information about size, sex, age and motivation. This variability could potentially be used to identify meagre in the field and to infer about ontogenetic phase (i.e., juveniles vs. adults, and variation with size) and motivation (e.g., spawning). Future studies should confirm sex differences and ascertain the influence of water temperature on acoustic features.
Evaluation of Sedative and Anxiolytic Activity of Methanolic Extract of Lablab purpureus (L.) Sweet Seeds in Experimental Mice Model
Authors: Ara Joushan , Hossain Md. Rabiul , Nizami Md. Saeedul Alam , Sayem Abu Sadat Md. 
Published: February 2020

Aims: Lablab purpureus (Fabaceae) a well-known Bangladeshi vegetable combines a great number of qualities with its adaptability and functional food properties. But still there is no evidance based report on neuropharmacological activities of Lablab purpureus seeds. For this reason, the present study was designed to analyze in vivo CNS effect of methanolic extract of Lablab purpureus seeds.
Place and Duration of Study: Department of Pharmacy, USTC between April 2017 and May 2018.
Methodology: In this study, the crude seeds extract of Lablab purpureus was evaluated for its sedative and anxiolytic effect using animal behavioral models, such as open field and hole cross tests for its sedative properties and an elevated plus maze (EPM) test for its anxiolytic potential.
Results: In open field and hole cross test, the seeds extract of Lablab purpureus showed a dose dependent suppression of motor activity and exploratory behaviour. In EPM test, animals treated with higher dose of methanolic extract showed significant (p<0.01) increased exploration to and time spent in EPM open arms.
Conclusion: This study provide in vivo evidence that the methanolic extract of Lablab purpureus has significant sedative and anxiolytic activity. Further studies on active constituent of the extract can provide approaches for therapeutic intervention.