bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of suture bridge technique in the treatment of acute patellar ligament injury
Authors: Zhou Ming , Zou Wen 
Published: September 2020

Introduction: The patellar ligament, as a knee extensor, plays an important role in knee movement. Patellar ligament tear completely divided into acute and chronic, acute general mishandling lead to fracture again, chronic patellar ligament rupture, need surgery to restore again and device, the integrity of the patellar ligament rupture time more than 2 weeks or more, it’s easy to have a different degree of scar healing, a normal tendinous portion elasticity and tension were significantly decreased, eventually leading to knee disorders. If scar tissue is removed by surgery and sutured again, the required strength of the patellar ligament cannot be provided, resulting in long postoperative recovery time, knee joint dysfunction, muscle atrophy, joint degeneration and other problems. Hypotheses: To explore the method and clinical effect of suture bridge technique in the treatment of acute patellar ligament injury. Methods: From January 2016 to October 2018, 13 patients with Acute injury of patellar ligament insertion were treated with suture bridge technique.Among them, there were 11 males and 2 females, aged from 17 to 35 years, with an average age of 26.3 years.The average time from injury to operation was from 3 to 5 days, with an average day of 3.5 days.Thirteen patients all suffered from closed injury at the inferior end of patella. Among them, 8 had no fracture at the inferior end of patella, 5 had bone fragments and none had avulsion at the tibial end.Thirteen patients all suffered from closed injury at the inferior end of patella. Among them, 8 had no fracture at the inferior end of patella, 5 had bone fragments and none had avulsion at the tibial end.Suture bridge technique was used in 13 cases.Postoperative knee function was assessed by Lysholm score Results: All patients were followed up for 7 to 15 months, with an average of 11.2 months.All incisions healed well, and there were no complications such as infection and re-rupture of patellar ligament.At 6-12 weeks after operation, the range of motion of 13 patients reached the level of uninjured side.According to Lysholm scoring criteria, knee joint function was excellent in 9 cases and good in 4 cases Conclusion: Suture bridge technique in the treatment of acute patellar ligament injury has the characteristics of firm fixation, fewer complications, quick recovery and avoidance of second operation.
Machine learning predicts rate of cartilage loss: data from the osteoarthritis initiative (OAI) and the multicenter osteoarthritis (most) studies
Authors: Paixão T. , DiFranco M. , Goetz C. , Ljuhar R. , Meier P. , Nehrer S. 
Published: September 2020

Introduction: Loss of cartilage is one of the hallmark radiographic symptoms of osteoarthritis and the direct cause of much of the disability directly related to OA. The rate of cartilage loss can range from a slow deterioration process, lasting decades, to a very rapid deterioration leading to complete loss in as little as 24 months 1. In fact, evidence has been gathering that a subset of OA patients develops an “accelerated” form of knee osteoarthritis 2. Hypotheses: The rate of cartilage loss can vary widely between patients at risk of or suffering from knee osteoarthritis (OA) but its causes remain unknown. We investigate prediction of future joint space width (JSW) loss from single time point quantitative and semi-quantitative radiographic features. Methods: Bilateral knee radiographs acquired at several time points in the context of the MOST study from 2651 patients (1079 female, 1572 male) were collected. Joint space narrowing (JSN), osteophyte and sclerosis OARSI grades, as well as Kellgren-Lawrence (KL) grade and joint space width were obtained from each image using an automated software algorithm. Individuals were classified as fast progressors if the rate of JSW loss, measured via linear regression, was above 10% baseline JSW. Fast progressors were predicted using a logistic regression model trained with KL and OARSI grades at baseline as independent variables. Independent validation was performed on 1900 individuals (1079 female, 821 male) from the Osteoarthritis Initiative (OAI) study. Performance was characterized by the area under the ROC curve (ROC-AUC). Confidence intervals were calculated by bootstrapping. Results: AUCs of 0.84 (0.82; 0.87) were achieved for classifying individual knees as fast progressors on the validation dataset (OAI). KL and sclerosis OARSI grades were the main predictors of rapid cartilage loss. Conclusion: We demonstrate prediction of future rapid cartilage loss from a single plain radiograph with validation on an independent dataset. Sclerosis OARSI grade, but not osteophytes OARSI grade, was a predictor of rapid cartilage loss, suggesting a non-canonical mode of OA progression.
Effect of tranexamic acid on ischemia － reperfusion injury caused by application of tourniquet in the surgery of total knee arthroplasty
Authors: Xiaofei Luo , Xuan Wie , Jinliang Wang 
Published: September 2020

Introduction: Tranexamic acid can effectively reduce the amount of perioperative bleeding in artificial joint replacement surgery, but some reports showing that the application of tranexamic acid in cardiac surgery can reduce the ischemia-reperfusion injury. Does tranexamic acid reduce the tourniquet Ischemia-reperfusion injury in the surgery of toutal knee arthroplasty is unclear. Hypotheses: To observe the effect of intravenous combined local tranexamic acid on the expression of IL-6 and TNF-α in peripheral blood and drainage fluid of patients with tourniquet in primary total knee arthroplasty Methods: Sixty patients with knee osteoarthritis were selected from 2017-07 to 2018-06 to proceed primary knee arthroplasty in our hospital. The random digital table was used to divide them into tranexamic acid group and normal saline group, with 30 cases in each group. In the tranexamic acid group, tranexamic acid 1g in 100ml normal saline was infused intraoperatively and at 3 h after operation. After incision, the infusion of tranexamic acid 1g in 100ml normal saline was performed. The saline group only 100 ml of physiological saline was infused during the same period of time, and 100 ml of physiological saline was locally perfused. The operation time, intraoperative blood loss, and drainage volume at 24 hours after operation were compared. The levels of IL-6 and TNF-α in the drainage fluid before and after surgery were compared between the two groups. Results: The drainage volume of tranexamic acid group was lower than that of normal saline group (P < 0.05). The levels of IL-6 and TNF-α in serum were lower than those in normal saline group at 24h and 72h after operation. The difference was statistically significant. (P < 0.05); the expression of IL-6 and TNF-α in the drainage fluid was significantly lower than that in the saline group at 24 hours after surgery (P < 0.05) Conclusion: Combined use of tranexamic acid can effectively reduce the expression of IL-6 and TNF-α in local drainage fluid and peripheral serum after TKA in patients with TKA, thus reducing the local and systemic inflammatory response.
Effect of unicompartmental knee arthroplasty and high tibial osteotomy with tomofix internal fixation in the treatment of unicompartmental knee osteoarthritis
Authors: Hou Yingzhou , Wang Shaohua , Wang Aiguo 
Published: September 2020

Introduction: Knee osteoarthritis is a common degenerative disease in the elderly clinically. Cartilage damage, osteophyte formation, joint space narrowing and bone exposure are the main pathological changes, mainly manifested as joint cartilage degeneration [1]. Since the knee joint load of normal people is mainly conducted through the medial side of the knee joint, it is easy to cause degeneration of the medial compartment and then narrow the joint space, which leads to the medial deviation of the lower limb line and the varus deformity of the knee joint. For patients aged 55-65 years with unilateral ventricular osteoarthritis of the knee, the current surgical methods are mostly high tibial osteotomy (HTO) or unicomartmental knee arthroplasty (UKA)[2,3]. HTO always thought to improve lower limb power line to correct deformities, effectively relieve pain and improve function, is an effective method for treatment of osteoarthritis knee inside, high cut bone is typically used in younger patients and patients from physical activity, can effectively reduce the load and delay of knee joint cartilage lesion replacement time, while UKA is more suitable for old age is not active, activity, and patients needs more intense in terms of pain relief. Hypotheses: To investigate the clinical effect of unicompartmental knee arthroplasty (UKA) and high tibial osteotomy (HTO) with tomofix internal fixation in the treatment of unicompartmental knee osteoarthritis. Methods: 60 patients with unicompartmental knee osteoarthritis and varus deformity of the knee joint were randomly divided into two groups: the UKA group (30 cases) and the HTO group (30 cases). The average follow-up time was 6 months, Scores preoperative and postoperative knee joint function, postoperative complications and postoperative pain satisfaction were compared. Results: The scores of HSS, VAS, femorotibial angle(FTA) and active range of motion(ROM) were 82.6 ± 12.9, 1.9 ± 0.8,173.2 ± 1.4,135.2 ± 1.6 in the group of unicompartmental knee arthroplasty (UKA);The scores of HSS, VAS(Visual Analogue Score), femorotibial angle(FTA) and active range of motion(ROM) after tomofix internal fixation used in the group of high tibial osteotomy (HTO)were 81.9 ± 14.3, 1.8 ± 0.9, 174.5 ± 1.8 and 121.1 ± 2.7 . There was no significant difference between the UKA group and HTO group in the scores of HSS, VAS and femorotibial angle(FTA) (P > 0.05). The active range of motion(ROM) of the HTO group was better than that of UKA group (P < 0.05). Conclusion: Both unicompartmental knee arthroplasty (UKA) and high tibial osteotomy (HTO) with tomofix internal fixation can improve the knee joint function and symptoms in the treatment of medial compartment osteoarthritis, but the active range of motion(ROM) in HTO group is better than UKA group.
The effect of combined cryotherapy/compression versus cryotherapy following ACL recon in return-to-sports after 3 months
Authors: Terai Sozaburo , Zantop Christian , Zantop Thore 
Published: September 2020

Introduction: Morbidity following ACL reconstruction may be a key factor contributing to muscular dysfunction and influencing physical activity. While intra-operative details have been the focus of standardization within the last years, strategies reducing the morbidity such as postoperative cryotherapy have been investigated less. Goal of this study was to compare subjective and objective patient outcomes following ACL reconstruction with combined compression and cryotherapy compared with traditional cryotherapy alone. Hypotheses: We hypothesized that cryotherapy/compression will show significantly better results three months after ACL reconstruction compared to cryotherapy alone. Methods: A total of 40 Patients undergoing ACL reconstruction are prospectively randomized to be treated with cryotherapy/compression device (GameReady, CA/USA, group 1) or a cryotherapy device (Aircast Cryocuff, DJO CA/USA, group 2). ACL reconstructions were performed by a single surgeon with semitendinosus graft and standardized rehabilitation protocol. Patients were instructed to use the ice therapy devices 4 times per day for 30min. Pain management directly postoperatively was analyzed as recorded during clinical records and VAS. Follow-up was performed at 12 weeks postoperatively using visual analog scale (VAS), Lysholm knee score, Short Form 36 (SF36), and Tegner acitiy score. Additionally, a functional “return-to-sports” analysis including isokinetic strength measurements (BTE-primus), proprioceptive tests (MTF tests) and a 3d-motion analysis (myomotion, Noraxon) during bilateral drop jumps and single leg hop tests was performed. Results: VAS and comparison of the administrated painkillers showed no significant differences between the two groups. Both groups used the cryotherapy/compression device and the cryotherapy device as determined by the study protocol. At 12 weeks, there was no significant difference between the two groups with regard to Lysholm score, Tegner score, and SF-36, however there was a trend towards better scores in group 1. The was a higher range of motion in group 1 compared to group 2. Functional analysis with isokinetic strength measurements, proprioceptive test and a 3d-motion analysis revealed better functional results in patients of the group cryotherapy/compression device compared to cryotherapy alone. Conclusion: This prospective analysis of 40 patients following semitendinosus ACL reconstruction with one single surgeon implicates that the use of a cryotherapy/compression device (GameReady) showed significantly better objective patient outcomes following ACL reconstruction compared to cryotherapy alone.
Functional outcome in patients following cartilage regeneration outcome at minimum follow-up of 12 months
Authors: Liefke Christian , Zantop Christian , Terai Shozaburo , Zantop Thore 
Published: September 2020

Introduction: Several studies have focused on the return to play rate after ACL reconstruction. Whereas most studies just report the rate as a result, only few studies evaluate specific parameters and tests to further investigate the conditions of when the patients should return to sports. Hypotheses: Aim of this study was to compare the return to competition rate of patients 12 months after ACL reconstruction and correlate the rate and re-rupture fate to the assessed multifactorial “return-to-sports” test 3 months after ACL reconstruction at our institution. Methods: A total of 51 Patients undergoing ACL reconstruction are prospectively evaluated using a “return-to-sports” test 3 months following ACL reconstruction. Inclusion criteria was isolated ACL rupture, Level 1 sports participation, free range of motion at FU 3 months, age >18 and <50, no associated ligamentous or meniscal injury influencing the rehab protocol and intact contralateral leg. ACL reconstructions were performed by a single surgeon with semitendinosus graft and standardized rehabilitation protocol. Follow-up at 3 months postoperatively was performed using a functional analysis including isokinetic strength measurements (BTE-primus), proprioceptive tests (MTF tests) and a 3d-motion analysis (myomotion, Noraxon) during bilateral drop jumps and single leg hop tests. To evaluate the return to play rate and recurrent instability problems a survey was used at timepoint 12 months postoperatively (n=43 at abstract submission). Results: At timepoint 12 months following ACL reconstruction 6 of 43 currently evaluated patients did not return to competition (6/43). A total of 5 patients were evaluated as moderate to high risk for ACL recurrent instability in the return-to-sports analysis at three months whereas one patient not returning to competition showed a not elevated risk for ACL recurrent instability in the analysis. The was a high correlation of patients revealing better functional results in the functional analysis (isokinetic strength, proprioceptive test and a 3d-motion analysis) and returning to sports. A total of 8 patients (4/43) suffered a recurrent instability after returning to sports. Conclusion: This prospective analysis of patients following semitendinosus ACL reconstruction with one single surgeon implicates that the rate of returning to sports in patients using a multifactorial “return-to-sports” analysis at 3 months postoperatively is higher compared to the rate of patients without such an analysis in the literature. We found a high correlation between better functional results and return to play rate as well as a re-rupture rate of 10% in patients after returning to sports.
Do the human gut metagenomic species possess the minimal set of core functionalities necessary for life?
Authors: Soverini Matteo , Rampelli Simone , Turroni Silvia , Brigidi Patrizia , Biagi Elena , Candela Marco 
Published: September 2020

Abstract
Background
Advances in bioinformatics recently allowed for the recovery of ‘metagenomes assembled genomes’ from human microbiome studies carried on with shotgun sequencing techniques. Such approach is used as a mean to discover new unclassified metagenomic species, putative biological entities having distinct metabolic traits.

Results
In the present analysis we compare 400 genomes from isolates available on NCBI database and 10,000 human gut metagenomic species, screening all of them for the presence of a minimal set of core functionalities necessary, but not sufficient, for life. As a result, the metagenome-assembled genomes resulted systematically depleted in genes encoding for essential functions apparently needed to support autonomous bacterial life.

Conclusions
The relevant degree of lacking core functionalities that we observed in metagenome-assembled genomes raises some concerns about the effective completeness of metagenome-assembled genomes, suggesting caution in extrapolating biological information about their metabolic propensity and ecology in a complex environment like the human gastrointestinal tract.
A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model
Authors: Brandon-Mong Guo-Jie , Shaw Grace Tzun-Wen , Chen Wei-Hsin , Chen Chien-Chang , Wang Daryi 
Published: September 2020

AbstractBackgroundNeuropathic pain is an abnormally increased sensitivity to pain, especially from mechanical or thermal stimuli. To date, the current pharmacological treatments for neuropathic pain are still unsatisfactory. The gut microbiota reportedly plays important roles in inducing neuropathic pain, so probiotics have also been used to treat it. However, the underlying questions around the interactions in and stability of the gut microbiota in a spared nerve injury-induced neuropathic pain model and the key microbes (i.e., the microbes that play critical roles) involved have not been answered. We collected 66 fecal samples over 2 weeks (three mice and 11 time points in spared nerve injury-induced neuropathic pain and Sham groups). The 16S rRNA gene was polymerase chain reaction amplified, sequenced on a MiSeq platform, and analyzed using a MOTHUR- UPARSE pipeline.ResultsHere we show that spared nerve injury-induced neuropathic pain alters gut microbial diversity in mice. We successfully constructed reliable microbial interaction networks using the Metagenomic Microbial Interaction Simulator (MetaMIS) and analyzed these networks based on 177,147 simulations. Interestingly, at a higher resolution, our results showed that spared nerve injury-induced neuropathic pain altered both the stability of the microbial community and the key microbes in a gut micro-ecosystem.Oscillospira, which was classified as a low-abundance and core microbe, was identified as the key microbe in the Sham group, whereasStaphylococcus, classified as a rare and non-core microbe, was identified as the key microbe in the spared nerve injury-induced neuropathic pain group.ConclusionsIn summary, our results provide novel experimental evidence that spared nerve injury-induced neuropathic pain reshapes gut microbial diversity, and alters the stability and key microbes in the gut.
The evaluation of red blood cell folate and methotrexate levels during protocol M in childhood acute lymphoblastic leukemia
Authors: Oosterom N. , Fiocco M. , Kloos R. Q. H. , van der Sluis I. M. , Pieters R. , van Zelst B. D. , Smith D. E. C. , van den Heuvel-Eibrink M. M. , de Jonge R. , Heil S. G. 
Published: September 2020

Abstract
Background
After High-Dose Methotrexate (HD-MTX), folinic acid rescue therapy (Leucovorin) is administered to reduce side effects in pediatric acute lymphoblastic leukemia (ALL) patients. Leucovorin and MTX are structural analogues, possibly competing for cellular transport and intracellular metabolism. We hypothesize that Leucovorin accumulates during consecutive courses, which might result in a lower MTX uptake.

Methods
We prospectively measured red blood cell (RBC) folate and MTX levels during four HD-MTX and Leucovorin courses in 43 patients treated according the DCOG ALL-11 protocol with 2-weekly HD-MTX (5 g/m2/dose) and Leucovorin (15 mg/m2/dose) using LC-MS/MS. We estimated a linear mixed model to assess the relationship between these variables over time.

Results
Both RBC MTX-PG and folate levels increased significantly during protocol M. MTX-PG2–5 levels increased most substantially after the first two HD-MTX courses (until median 113.0 nmol/L, IQR 76.8–165.2) after which levels plateaued during the 3d and 4th course (until median 141.3 nmol/L, IQR 100.2–190.2). In parallel, folate levels increased most substantially after the first two HD-MTX courses (until median 401.6 nmol/L, IQR 163.3–594.2) after which levels plateaued during the 3d and 4th course (until median 411.5 nmol/L, IQR 240.3–665.6). The ratio folate/MTX-PG decreased significantly over time, which was mostly due to the relatively higher increase (delta) of MTX-PG.

Conclusion
These results suggest that the increase in RBC folate levels does not seem to have a large effect on RBC MTX levels. Future studies, assessing competition of Leucovorin and MTX on other cellular mechanisms which might negatively affect treatment efficacy, are necessary.
Development and validation of a screening instrument for borderline personality disorder (SI-Bord) for use among university students
Authors: Lohanan Trustsavin , Leesawat Thanakorn , Wongpakaran Tinakon , Wongpakaran Nahathai , Karawekpanyawong Nuntaporn , Oon-Arom Awirut , Kuntawong Pimolpun 
Published: September 2020

Abstract
Background
The screening instrument for borderline personality disorder (SI-Bord) consists of a 5-item self-reported questionnaire on the key features of BPD from the DSM-5 using a 5-point Likert scale. This study investigated its validity and reliability in screening for BPD in university students.

Methods
A cross-sectional study was conducted on a sample of university students in Thailand between November and December 2019. An online assessment gathered demographic data and results from the SI-Bord, the Perceived Stress Scale-10 (PSS-10) and the Patient Health Questionnaire-9 (PHQ-9). Participants whose SI-Bord scores were ≥ 1 were randomly selected to be interviewed and assessed for a BPD diagnosis by four psychiatrists using the Structured Clinical Interview for DSM-IV Axis II Personality Disorders (SCID-II) as a reference point. An intraclass correlation coefficient (ICC) of 0.925 (95% CI, 0.805–0.979) ensured inter-rater reliability between the four psychiatrists. The diagnostic sensitivity and specificity of the SI-Bord, as compared to that of the SCID-II, were determined to indicate the cut-off score. The Receiver Operating Characteristics (ROC) was analyzed to evaluate its diagnostic accuracy.

Results
The study included 342 students aged 18–25 years (the mean age was 20.25 ± 1.4 years), 80.4% of whom were female. Among the 68 participants selected for an online interview, 16 were diagnosed with BPD. The cut-off score of the SI-Bord was > 9, as suggested by the Youden index, yielding a sensitivity of 56.3% and a specificity of 92.3%. It had a positive predictive value of 69.2% and negative predictive value of 87.3%. The SI-Bord had adequate discriminative power between cases and non-cases of BPD, with the area under the ROC curve being 0.83. Cronbach’s alpha for the SI-Bord was 0.76, indicating acceptable internal consistency. The SI-Bord score was positively correlated to PHQ-9 and PSS-10 scores (r = 0.67 and r = 0.69, p < 0.001, respectively) and negatively correlated to MSPSS (r = − 0.50, p < 0.001). The prevalence of BPD in the sample was 6.4%, according to the cut-off score > 9.

Conclusion
The SI-Bord demonstrated good reliability and validity for screening BPD in university students. However, a study in non-Thai and other population groups should be warranted.