bluuebunny/crossref_metadata_2025_split huggingface dataset:
Description of a Brain Neuropeptidome of the Malaria Vector Nyssorhynchus Albimanus and Neuropeptide Differential Expression During Infection with Plasmodium Berghei
Authors: Alvarado-Delgado Alejandro , Martínez-Barnetche Jesus , Tellez-Sosa Juan , Rodríguez Mario Henry , Gutiérrez-Millan Everardo , Zumaya-Estrada Federico , Saldaña-Nabor Vianey , Rodríguez Maria del Carmen , Tello-López Angel , Lanz-Mendoza Humberto 
Published: May 2020

Abstract
Background Insect neuropeptides, mainly synthesized in the brain, play a central role in the control of many physiological processes. A neuropeptidome of the mosquito Nyssorhynchus albimanus was described based in a comparative analysis of the mosquito genome complemented with high-throughput sequencing of brain transcriptomes. In addition, neuropeptides differentially expressed during Plasmodium infection were identified.Results We identified 3,811 transcripts associated to translation, oxidation-reduction process, protein binding, ATP binding, integral components of membrane and ribosome, among others. We identified 29 neuropeptide transcripts that predicted at least 60 biopeptides, including pyrokinin, glycoprotein hormone alpha (GPA2), prothoracicotropic hormone, neuropeptide-like precursor 1 (NPLP1), allatostatin C, orcokinin, corazonin, adipokinetic hormone I, SIFamide, pyrokinin capa-like, pigment-dispersing factor, adipokinetic hormone/corazonin-related peptide (ACP), tachykinin-related peptide, trissin, neuropeptide F, short neuropeptide F (sNPF), diuretic hormone 31, bursicon, crustacean cardioactive peptide (CCAP), allatotropin, allatostatin 1, partner of bursicon (PBURS), ecdysis triggering hormone (ETH), diuretic hormone 44 (Dh44), insulin-like peptides 5, 1, 3, 7, 2 (ILPs) and eclosion hormone (EH) and seven neuropeptide receptors. Transcript mapping to the Ny. albimanus genome provided evidence for the re-annotation of the myosuppressin gene. A quantitative analysis documented increased expression of adipokinetic hormone/corazonin-related peptide, pyrokinin and corazonin in the mosquito brain after Plasmodium berghei infection.Conclusion This work represents an initial effort to characterize the neuropeptide repertoire of Ny. albimanus and provides new information for understanding neuroregulation of the mosquito response during Plasmodium infection .
WITHDRAWN: Aggressive Vertebral Hemangiomas Causing Compression Fracture with Neurological Deficits: A Retrospective Cohort Study
Authors: Rao Hongming , Li Guishuang , Liu Limin , Huang Yuming , Xu Zhengquan , Xu Weihong 
Published: May 2020

Abstract
Background:In rare instances, aggressive vertebral hemangiomas (VHs) can cause compression fracture, resulting in severe pain and neurological deficits. But the diagnosis and treatment of these aggressive lesions are challenging because of these lesions are rare and atypical. This study aimed to evaluate the safety and efficacy of surgical management for aggressive VHs with vertebral compression fracture by a modified multimodality surgery.Methods:We retrospectively reviewed 6 cases suffering from aggressive VHs with compression fracture and neurological deficits in our department from July 2011 to April 2016. These patients were treated by the multimodality surgery, includingpreoperative embolization, intraoperative injection of gelfoam mixed with cement, and laminectomy decompression.The follow-up period was at least 3 years. Perioperative parameters, clinical outcomes, and radiographical data were collected and analyzed.Results: The 6 patients involved 1 male and 5 females (mean age, 52.3 years).The levels involved were: in thoracic spine (5 cases) and lumbar spine (1 case).Preoperative CT-guided biopsy was conducted in all patients, with 5 patients had definitive pathologic diagnosis. All patients were treated successfully with the multimodal surgery, with no cement leakage and other severe complications. The mean operation time was 182.2 minutes, and the estimated blood loss was 908.3 ml.At an average follow-up of 49.8 months, clinical outcomes assessed by the visual analogue scale and Frankel grade were significantly improved. The vertebral body height and kyphosis angle of the fractured vertebra were also corrected postoperatively. No affected vertebra re-fracture and adjacent vertebral fracture were developed, and none of the patients experienced recurrence of tumor at final follow-up.Conclusions:In cases of aggressive VHs causing compression fracture with neurological deficits, CT-guided biopsy is indicated for the diagnosis. The multimodality surgery (preoperative embolization, intraoperative injection of gelfoam mixed with bone cement combined laminectomy decompression) is effective and safe, and can be considered as an acceptable surgical choice.
Clinical observation of glucocorticoids application in patients with severe coronavirus disease 2019 (COVID-19): a descriptive study
Authors: Yue Linli , Wang Xuan , Han Lefei , Yang Lin , Li Xiaolan , Wan Zhenzhen , Li Na , Jiang Hong 
Published: May 2020

Abstract
Background

The coronavirus disease 2019 (COVID-19) caused an ongoing pandemic and bring heavy burden globally. We retrospectively investigated the effect of glucocorticoids on clinical symptoms, course of disease, and negative change of nucleic acid detection in patients with severe COVID-19.
Methods

Individual demographics data, CT images, clinical outcomes, laboratory investigations, treatment of patients with severe COVID-19 were collected from electronic medical records of the Central Hospital of Wuhan, from January 28 to February 28, 2020.
Results

Eighteen severe cases of COVID-19 received low-dose of glucocorticoid treatments. They were initially treated with 40 mg methylprednisolone with gradually reduced doses and changed into oral prednisolone. The courses of glucocorticoid treatments ranged from 4 to 30 days. The pulmonary infections were absorbed to different degrees in 16 cases, one case deteriorated in CT imaging and one had no significant change. All cases had fever regression. Fifteen cases could respire without oxygen supply. Two were still inhaled oxygen through nasal catheters and one with high flow oxygen gradually transferred to face mask for oxygen supply. Five cases discharged. Twelve cases who were in stable conditions for treatment remained in hospital. One case was still in critical condition. The results of three re-examinations of SARS-Cov-2 nucleic acids by RT-PCR were negative.
Conclusions

The early use of low-dose glucocorticoid therapy in severe COVID-19 infection may have a positive effect on the prevention and treatment of disease deterioration.
Changes of Deltoid Tissue Properties after Reserve Shoulder Arthroplasty – A Shear Wave Elastography Study
Authors: Fenwick Anabell , Reichel Thomas , Eden Lars , Schmalzl Jonas , Meffert Rainer H , Boehm Dirk , Plumhoff Piet , Gilbert Fabian 
Published: May 2020

Abstract
IntroductionThe deltoid muscle plays an essential role for the postoperative functional outcome after reverse shoulder arthroplasty. Ultrasound elastography is areliable method for detecting soft tissueproperties and their changes due to different conditions or pathologies. Tissue stiffness is calculated after application of an acoustic impulse (acoustic radiation force impulse, ARFI), which deforms the underlying tissue. The purpose of this study was to evaluate changes in the deltoid muscle tissue after RSA and reveal a correlation to the functional outcome.Material and methods18 patients treated with reserve shoulder arthroplasty after proximal humeral fracture or cuff arthropathy were included. Shear wave elastography was performed on both sides of three regions of the deltoid muscle. Functional outcome was recorded by VAS, CMS, range of motion and strength (flexibar). Statistical analysis was performed by SPSS.ResultsThe functional outcome was satisfactory (VAS pain: 18 points, VAS function: 64 points, Constant Murley: 66 points). Force was reduced to 48.8N after RTSA in comparison to 58.3 N on the healthy side (p> 0.07). SWE was able to show a higher overall muscle tension of the deltoid in patients after RSA compared to the contralateral non-operated side. The differences were particularly visible in the anterior and middle deltoid region. Under isometric load all deltoid regions showed significantly increased tension, which was particularly eminent in the anterior region of the pars clavicularis.ConclusionShear wave elastography is a reliable method for detecting changes of tension in the deltoid muscle after RTSA and shows a higher tension of all areas of the deltoid, especially prominent in the anterior and middle portion of the deltoid. Future application possibilities are monitoring of deltoid vitality, preoperative assessment and intraoperative management.Level of evidence: diagnostic study, Level III
A Patient-specific Navigational Template for Precise Acetabular Placement in Unilateral Total Hip Arthroplasty: A Cadaveric Study
Authors: LIANG Jinlong , Gao Xinjian , Fang Xuewei , Zhao Yonghui , Xu Yongqing , Lu Sheng 
Published: May 2020

Abstract
Background Total hip arthroplasty (THA) is a widely performed reconstructive surgical intervention. In this paper, we describe a novel patient-specific navigational template to assist in acetabular component implantation in unilateral THA. Methods The template was produced based on data preoperatively acquired with computed tomography (CT) scan. We used the mirror image of the healthy contralateral acetabular anatomical structure to ensure accurate acetabular component implantation in unilateral THA. The surface of the template was designed to conform to the unique contours of the cadaveric acetabular fossa by reverse engineering technology. The orientation of the navigation channel was defined by the acetabular central axis which was determined by the contralateral acetabular centre of rotation, anteversion angle and abduction angle. Each template was formed from acrylate resin by using rapid prototyping (RP) technique. Finally, the template was tested in 20 cadavers scheduled for unilateral THA and postoperative medical imaging was used to evaluate the accuracy and validity of the template. Results During the operation, the acetabular fossa template was easy to obtain in all cases. The abduction angle ( β ) of the cup was (49.9°±4.1°) versus (49.5°±4.7°) on the contralateral side. The anteversion angle (α) of the cup was (17.7° ± 3.1°) versus (18.3°±3.5°) on the contralateral side. In the operative hip, the height of the prosthesis centre(H)was (21.6 mm±2.8 mm) versus (21.9 mm±3.4 mm) in the contralateral side, and the horizontal location of the prosthesis centre(W) was (29.7 mm±3.1 mm) versus (30.90 mm±3.31 mm) in the contralateral side. There was no significant difference in the cup abduction (β) or anteversion (α) angle between the operative and contralateral sides (P=0.7531>0.05 for β and P=0.5996>0.05 for α); In addition, there was no significant difference in the height(H)or horizontal location(W) of the acetabular centre between the operative and contralateral sides (P=0.6494>0.05 for W and P=0.5143>0.05 for H). Conclusion The navigational template is a promising tool for facilitating preoperative planning and intraoperative techniques. With the aid of the template, an acetabular prosthesis can be precisely implanted to the expected position in unilateral THA.
The surgical treatment of retroperitoneal echinococcosis: the experience of a single center
Authors: He Jingyu , Yang Chuang , Qiu Yiwen , Wang Wentao 
Published: May 2020

Abstract
Background

Echinococcosis refers to a worldwide epidemic of zoonotic parasites that commonly affect the human liver, lungs, and omentum and can be classified as cystic echinococcosis (CE) and alveolar echinococcosis (AE).Retroperitoneal echinococcosis (RE) is a rare condition that is associated with a high mortality and disability rate. Because RE manifests in a concealed and deep location, and can often involve several important organs, it is associated with a high rate of misdiagnosis, a high risk of surgery, and is extremely difficult to manage.
Methods

This was a retrospective analysis of the characteristics and surgical management of patients diagnosed with RE in our hospital between 2012 and 2019.
Results

Between 2012 and 2019, 1257 cases of echinococcosis and 121 cases of RE were diagnosed in our hospital. Of these, 68 cases involved surgical treatment, 53 involved non-surgical treatment, and 12 cases were lost to follow-up (four cases in the surgical group and eight cases in the non-surgical group). Thus, 109 cases were followed-up. RE cases were divided according to different treatment methods into a radical resection group (Group A, 31 cases), a non-radical resection group (Group B, 37 cases), and a non-surgical group (Group C, 53 cases). We carried out a detailed analysis of the 109 cases experiencing surgical intervention with effective follow-up; there were 31 cases of radical resection (Group A), 33 cases of non-radical resection (Group B).
Conclusions

Our analysis found that RE is rare and can occur at any age; the cystic form is common and often involves multiple organs. The liver is the most commonly affected organ and is associated with serious complications. The rate of radical resection for RE is low, and multiple organ resection is often required; there is a high incidence of postoperative complications. Radical resection is the first line of treatment of RE, although non-radical surgery can benefit most patients. It is important to emphasize the importance of the first round of surgery, particularly in cases involving hepatic echinococcosis. If the lesion can be removed radically during the first round of surgery, then radical surgery should be performed.
The Development and Validation of a Questionnaire in Bahasa Malaysia to Assess Problems of Vision or Feelings About Vision Among Patients with Cataracts.
Authors: Abas Adinegara Lutfi , Thevi Thanigasalam , Chang Stephanie Yen Li 
Published: May 2020

Abstract
Purpose: We conducted the study to compare the validation properties of the English version of the Questionnaire and the Bahasa Malaysia (Malay Language) version regarding the Quality of Life of patients with cataracts.Methods: We carried out a cross-sectional study collecting data between June 2017 and March 2018 in the pre-operative Eye Clinic of Hospital Melaka.The Malay version was translated by two independent translators who were well versed in both languages. Methods used to validate the standard questionnaire included the use of construct validity via factor analysis and the deployment of reliability test through assessment of internal consistency via Cronbach’s alpha. Results: We observed both English and Bahasa Malaysia versions to have high reliability with Cronbach’s alpha values of 0.90 and above in factors on difficulty with activities and responses to vision problems.Exploratory factor analysis performed revealed that the three-factor model fits the data well for the English version of the questionnaire - difficulty with activities (23.81% of variance), responses to vision problem (22.22% of variance) and general health and vision (14.68% of variance). The Bahasa Malaysia version of the questionnaire produced three factors with two of the factors resembling the factors from the original version of the questionnaire - difficulty with activities (24.28% of variance) and responses to vision problem (23.66% of variance). Conclusion: The present study observed that both the English and Bahasa Malaysia versions of the NEI VFQ-25 have comparable construct validity to the original American version. With high validity and reliability, the tool shall be able to provide health care providers the assessment of impact due to cataract and other ophthalmic conditions on the vision-related quality of life of ophthalmic patients.
The Association Between Mental Health, Chronic Disease, and Retired Status Among Korean Elderly: A Cross-Sectional Study
Authors: Kim Tae Yoon , Lee Sook-Hyun , Kim Eun-Jung , Kim Jae-Hong , Chung Sun Yong , Ha In-Hyuk 
Published: May 2020

Abstract
Background Retired status has strong associations with mental health factors and involves gender disparities. However, while retirement is known to affect the mental health of the elderly, large-scale studies examining the impact on mental health by gender are scarce.Methods This cross-sectional study examined the data of 1,664 participants aged over 65 years old who completed the economic status and mental health sections of the 6-7th Korean National Health and Nutrition Examination Surveys (2013–2017). We adjusted for covariates (health behavior, sociodemographic factors) in regression analysis for complex sampling design to calculate regression coefficients and 95% confidence intervals of the link between retired status and mental health. We also assessed which covariates of chronic disease, health behavior, and sociodemographic factors weakened the correlation between retired status and mental health.Results Retired status in Korean populations has persisted to negatively impact the incidence of depressive symptoms and thoughts of suicide, even after adjusting for confounding variables. Regarding mental health, women were affected by retired status to a greater extent than men. Among covariates, health behaviors and sociodemographic factors were most affected by retired status. Women were more affected by household income, and men were more affected by drinking behaviors.Conclusions These results imply that chronic diseases and psychological factors should be managed delicately among the retired elderly, and that there may be gender differences in retirement adaptation.
Growth, yield and grain nutrients response of wheat (Triticum aestivum L.) to biochar, lime and farmyard manure amendment of the croplands in southern Ethiopia.
Authors: lulu mekdes , Lemma Bekele , Hidoto Legesse 
Published: May 2020

Abstract
Background: Soil fertility decline in agricultural land is due to intensive cropping system, shortening of the fallow period, reduced manure application, extensive use of crop residues as fuelor fodder and removal of ground cover. This study investigated the effect of biochar (BC), lime and farmyard manure (FYM) on growth, yield and grain nutrient of wheat on two cropland sites in southern Ethiopia. Result: The effect of site was significant (P < 0.05) for most parameters investigated, indicating the influence of soil type. The effects of BC, lime, and FYM were significant (p ≤ 0.001) on straw yield, grain yield, and total biomass. However, BC (5 tha -1 ) produced the highest straw yield, grain yield, and total biomass. Similarly, BC and lime significantly (P < 0.001) affected plant height, and tiller numbers and productivity. BC (5 tha -1 ) gave the highest plant height, total tillers, and productive tillers, maximum number of seeds per spike, while FYM (10 tha -1 ) produced the highest spike length. Biochar significantly (P < 0.05) increased the concentrations of P and K in wheat grain. The highest effect on grain Ca concentration was from the application of lime while the highest on grain N concentration was from FYM (10 tha -1 ). Conclusion: The effect from BC was attributed to improvements in soil pH, CEC, nutrient availability and water retention. Thus BC has a good potential as a soil amendment for smallholder farm crop production.
Quality conceptualization in the era of measurement: A qualitative study of primary care physicians in Medicare’s Merit-Based Incentive Payment System
Authors: Berdahl Carl Thomas , Easterlin Molly C , Ryan Gery , Needleman Jack , Nuckols Teryl K 
Published: May 2020

Abstract
Background: While governmental programs seeking to improve the quality and value of healthcare through pay-for-performance initiatives have good intentions, participating physicians may be reluctant to participate for various reasons, including poor program alignment with considerations relevant to daily clinical practice. In this study, we sought to characterize how primary care physicians (PCPs) participating in Medicare’s Merit-Based Incentive Payment System (MIPS) conceptualize the quality of healthcare to help inform future measurement strategies that physicians would understand and appreciate.Methods: We performed semi-structured qualitative interviews with a nationwide sample of 20 PCPs participating in MIPS who were trained in internal medicine or family medicine. We asked PCPs how they would characterize quality in healthcare and what distinguished exceptional, good, and poor quality from one another. Interviews were transcribed and two coders independently read transcripts, allowing data to emerge from the interviews and developing theories about the data. The coders met intermittently to discuss findings, harmonize the coding scheme, develop a final list of themes and sub-themes, and aggregate a list of representative quotations.Results: Participants described quality in healthcare as consisting of two components: (1) evidence-based care that is safe, which included appropriate health maintenance and chronic disease control, accurate diagnoses, and adherence to guidelines and (2) patient-centered care, which included spending enough time with patients, responding to patient concerns, and establishing long-term patient-physician relationships that were founded upon trust. Conclusions: PCPs consider patient-centered care to be necessary for the provision of exceptional quality in healthcare. Program administrators for quality measurement and pay-for-performance programs should explore new ways to reward PCPs for providing outstanding patient-centered care. Future research should be undertaken to determine whether patient-centered activities such as forging long-term, favorable patient-physician relationships, are associated with improved health outcomes.
Surgical Treatment for Avulsion Fractures of the Anterolateral Ligament Associated with Periarticular Fractures of the Knee
Authors: Wei Xuelei , Wang Zengliang , Lu Yandong , Sun Jie , Riehl John 
Published: May 2020

Abstract
Background: To confirm the existence of the ALL and evaluate the clinical outcomes of surgical management for avulsion fractures involving its insertion when associated with periarticular knee fractures.Methods: Twenty-three patients (16 males and 7 females) with avulsion fractures of the ALL associated with periarticular knee fractures were fixed with a spider plate, cannulated screw, or suture anchor. Eight patients were diagnosed with distal femoral fracture, 10 with tibial plateau fracture, and 5 with tibial eminence avulsion fracture. All patients underwent X-rays at follow-up. Clinical and functional outcomes were assessed with the pivot-shift test, objective and subjective International Knee Documentation Committee (IKDC) score, Lysholm score, and Tegner activity scale.Results: The ALL was found and identified as a distinct ligamentous structure in all patients. Pre-Segond repair, patients had significantly more instability as determined by pivot-shift test than seen postoperatively (P < .0001). At final follow-up, the mean subjective IKDC score was 83.2±10.3. Fourteen patients were graded A, 6 were graded B, and 3 was graded C on the IKDC objective score. The mean Lysholm score was 85.4 ± 12.2. The mean Tegner score was 7.5 ± 1.2.Conclusion: This study confirmed that the ALL is a distinct structure in the anterolateral portion of the knee. The fixation for the avulsion fracture of the ALL associated with periarticular knee fractures can be an effective procedure without specific complications. Longer term and comparative follow-up studies are necessary to confirm the effects.