bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel method for isolation and culture of primary swine gastric epithelial cells
Authors: Bautista-Amorocho Henry , Silva-Sayago Jorge Alexander , Goyeneche-Patino Diego A. , Pérez-Cala Tania Liseth , Macías-Gómez Fabio , Arango-Viana Juan Carlos , Martínez Alonso 
Published: January 2021

Abstract
Background: Culturing primary epithelial cells has a major advantage over tumor-derived or immortalized cell lines as long as their functional phenotype and genetic makeup are mainly maintained. The swine model has shown to be helpful and reliable when used as a surrogate model for human diseases. Several porcine cell lines have been established based on a variety of tissues, which have shown to extensively contribute to the current understanding of several pathologies, especially cancer. However, protocols for the isolation and culture of swine gastric epithelial cells that preserve cell phenotype are rather limited. We aimed to develop a new method for establishing a primary epithelial cell culture from the fundic gland region of the pig stomach.Results: Mechanical and enzymatic dissociation of gastric tissue was possible by combining collagenase type I and dispase II, protease inhibitors and antioxidants, which allowed the isolation of epithelial cells from the porcine fundic glands showing cell viability > 90% during the incubation period. Gastric epithelial cells cultured in RPMI 1640, DMEM-HG and DMEM/F12 media did not contribute enough to cell adhesion, cluster formation and cell proliferation. By contrast, William’s E medium supplemented with growth factors supports confluency and proliferation of a pure epithelial cell monolayer after 10 days of incubation at 37oC, 5% CO2. Mucin-producing cell phenotype of primary isolates was confirmed by PAS staining, MUC1 by immunohistochemistry, as well as the expression of MUC1 and MUC20 genes by RT-PCR and cDNA sequencing. Swine gastric epithelial cells also showed origin-specific markers such as cytokeratin cocktail (AE1/AE3) and cytokeratin 18 (CK-18) using immunohistochemical and immunofluorescence methods, respectively.Conclusions: A new method was successfully established for the isolation of primary gastric epithelial cells from the fundic gland zone through a swine model based on a combination of tissue-specific proteases, protease inhibitors and antioxidants after mechanical cell dissociation. The formulation of William’s E medium with growth factors for epithelial cells contributes to cell adhesion and preserves functional primary cells phenotype, which is confirmed by mucin production and expression of typical epithelial markers over time.
Heterogeneity of Borderline Personality Disorder Symptoms in Help-Seeking Adolescents
Authors: Cavelti Marialuisa , Lerch Stefan , Ghinea Denisa , Fischer-Waldschmidt Gloria , Resch Franz , Koenig Julian , Kaess Michael 
Published: January 2021

Abstract
Background: The heterogeneous presentation of borderline personality disorder (BPD) represents a clinical challenge. There is an ongoing scientific debate whether the heterogeneity can best be understood in terms of qualitative (categorical) or quantitative (dimensional) differences between individuals. The present study examined the latent structure of BPD in adolescents. Methods: Five-hundred and six outpatients aged 12 to 17 years with risk-taking and/or self-harming behavior were assessed at baseline and one-year follow-up. Latent class analysis (corresponding with the categorical approach), factor analysis (corresponding with the dimensional approach), and factor mixture models (allowing for both categorical and dimensional aspects) were applied to the DSM-IV BPD criteria.Results: The best fitting model distinguished between a majority class with high probabilities for all BPD criteria (“borderline group”) and a minority class with high probabilities for the impulsivity and anger criteria only (“impulsive group”). Sex significantly affected latent class membership, and both a latent factor and age explained within-class variability. The borderline group primarily consisted of females, frequently reported adverse childhood experiences, scored high on the emotion dysregulation and inhibitedness personality traits, and was associated with internalizing psychopathology. In contrast, the impulsive group primarily consisted of males, scored high on the dissocial behavior personality trait, and was associated with externalizing psychopathology. After one year, the two groups showed similar clinical improvement.Conclusions: The study provides evidence for two distinct subgroups of adolescents with BPD features that resemble the subtypes of the ICD-10 emotionally unstable personality disorder. More research is needed to further investigate the diagnostic stability of the two groups over time and potential differential treatment indications.
Comparison of functional and oncological outcomes of innovative “three-port” and traditional “four-port” laparoscopic radical prostatectomy in patients with prostate cancer
Authors: Xu Ben , Cheng Si-da , Peng Yi-ji , Zhang Qian 
Published: January 2021

Abstract
Background: To compare the functional and oncological outcomes between innovative “three-port” and traditional “four-port” laparoscopic radical prostatectomy (LRP) in patients with prostate cancer (PCa).Methods: We retrospectively collected the data of PCa patients treated at our institutions from June 2012 to May 2016. According to the inclusion criteria, a total of 234 patients were included in the study, including 112 in group A (four-port) and 122 in group B (three-port). The perioperatively surgical characteristics, functional and oncological outcomes were compared between groups.Results: There were no statistical differences in the baseline parameters between these two groups. Compared with group A, the operative time (OT) and estimated blood loss (EBL) were significantly less in group B. On follow-up, the rate of positive surgical margin (PSM), prostate specific antigen (PSA) biochemical recurrence and continence after LRP did not show any statistically significant difference between the groups. An identical conclusion was also received in comparison of overall survival (OS) and biochemical recurrence-free survival (BRFS) between both groups. Conclusion: Innovative “three-port” LRP can significantly shorten the OT and reduce the EBL compared with the traditional “four-port” LRP. Meanwhile, it does not increase the rate of PSM and PSA biochemical recurrence. “Three-port” LRP could be popularized in the future in view of its superior surgical technique, considerably better functional outcomes and remarkable oncological control.
Factors associated with ocular surface epithelial damage in patients with primary Sjögren's syndrome
Authors: Kim Ji Eun , Kim Na Rae , Chin Hee Seung , Seo Kyoung Yul , Kim Tae Im , Jung Ji Won 
Published: January 2021

Abstract
Background: The aim of this study was to evaluate the effects of systemic parameters, laboratory findings, oral parameters, and other ocular surface parameters on ocular surface epithelial damage in patients with primary Sjögren's syndrome (pSS).Methods: A total of 82 dry eye disease (DED) patients with pSS were enrolled in this study. Ocular surface epithelial damage was measured by ocular staining score (OSS). Systemic parameters, laboratory findings including serologic markers, oral parameters, and other ocular surface parameters were collected. Other ocular surface parameter assessments such as the Schirmer’s test, fluorescein tear breakup time, meibomian gland examinations, noninvasive keratographic tear film break-up time measurements using the Keratograph® 5M were performed, and the Ocular Surface Disease Index was determined. Results: In a multivariate analysis, decreased age and increased duration of pSS were significantly related to increased logarithm-transformed OSS (β = -0.011, P = 0.043 for and β = 0.003, P = 0.008). Among the ocular surface parameters, decreased fluorescein tear breakup time and increased MGD grade were significantly associated with increased logarithm-transformed OSS (β = -0.183, P < 0.001 and β = 0.192, P = 0.049).Conclusions: Ocular surface epithelial damage in patients with pSS was associated with young age, long duration of disease, unstable tear film, and decreased meibomian gland function.
Choice-dependent cross-modal interaction in the medial prefrontal cortex of rats
Authors: Zheng Mengyao , Xu Jinghong , Keniston Les , Wu Jing , Chang Song , Yu Liping 
Published: January 2021

Abstract
Cross-modal interaction (CMI) could significantly influence the perceptional or decision-making process in many circumstances. However, it remains poorly understood what integrative strategies are employed by the brain to deal with different task contexts. To explore it, we examined neural activities of the medial prefrontal cortex (mPFC) of rats performing cue-guided two-alternative forced-choice tasks. In a task requiring rats to discriminate stimuli based on auditory cue, the simultaneous presentation of an uninformative visual cue substantially strengthened mPFC neurons' capability of auditory discrimination mainly through enhancing the response to the preferred cue. Doing this also increased the number of neurons revealing a cue preference. If the task was changed slightly and a visual cue, like the auditory, denoted a specific behavioral direction, mPFC neurons frequently showed a different CMI pattern with an effect of cross-modal enhancement best evoked in information-congruent multisensory trials. In a choice free task, however, the majority of neurons failed to show a cross-modal enhancement effect and cue preference. These results indicate that CMI at the neuronal level is context-dependent in a way that differs from what has been shown in previous studies.
‘Walking Wombs’: Making Sense of the Muskoka Initiative and the Emphasis on Motherhood in Canadian Foreign Policy
Authors: Tiessen Rebecca 
Published: January 2021

The Muskoka Initiative – or the Maternal, Newborn and Child Health (MNCH) Initiative has been a flagship foreign policy strategy of the Harper Conservatives since it was introduced in 2010. However, the maternal health initiative has been met with a number of key criticisms in relation to its failure to address the sexual and reproductive health needs of women in the Global South2. In this article, I examine these criticisms and expose the prevalent and problematic discourse employed in Canadian policy papers and official government speeches pertaining to the MNCH Initiative. I examine the embodiment of the MNCH and how these references to women’s bodies as “walking wombs” facilitate: the objectification and ‘othering’ of women as mothers and childbearers; a discourse of ‘saving mothers’ in a paternalistic and essentialist language; and the purposeful omission of gender equality. Feminist International Relations (IR) and post-colonial literature, as well as critical/feminist Canadian foreign policy scholarship are employed in this paper to frame these critiques.
Transdermal Buprenorphine-A Novel Medication for Postoperative Pain Management Following Abdominal Hysterectomy for Benign Gynecological Conditions
Authors: Yevale Aarti , Shetty Jyothi , Poojari Vidyashree G. , Guddattu Vasudeva 
Published: January 2021

Background:
Buprenorphine is a semisynthetic derivative of thebaine, which is 75-100
times more potent than morphine and causes less respiratory depression. Efficacy and safety of
transdermal buprenorphine have been well established in chronic pain, but data regarding acute
postoperative pain relief are still limited.

Aim:
In this study, we compared the efficacy of transdermal buprenorphine patch with injectable
tramadol for the relief of postoperative pain (POP) following abdominal hysterectomy for benign
gynecological conditions.

Settings and Design:
Three-year prospective observational study.

Methods and Material:
One hundred patients undergoing abdominal hysterectomy were allocated
to one of two groups. Buprenorphine group: we applied 10 mcg/hr transdermal buprenorphine patch
on the lateral upper arm after surgery. The onset of relief from a patch is 8 to 16 hours. So, all patients
were given diclofenac suppository 100mg before induction of anesthesia as a preemptive
analgesic to reduce postoperative pain.

Injectable Tramadol Group:
Received intramuscular tramadol injections 50mg every 8 hours in the
postoperative period. Patients were followed for two days to observe postoperative pain relief and
adverse effects. If patients showed a Visual Analog pain (VAS) score >3, intravenous paracetamol
was administered as a rescue analgesic.

Results:
POP was assessed at different time frames: 12, 24, 36 and 48 hours after hysterectomy.
Mean pain score at rest in the buprenorphine group was significantly lower than that of the tramadol
group. Patients experienced less pain on mobility in the buprenorphine group compared with the
tramadol group, which was statistically significant.

Conclusion:
Transdermal buprenorphine is an effective and safe alternative to injectable tramadol
for providing postoperative analgesia. It provides a higher degree of pain relief, faster postoperative
mobilization, lower incidence of postoperative nausea and vomiting and higher satisfaction scores
starting 12 hours after surgery. However, for the first 12 post-operative hours, there is a need for
additional analgesia.
Remix and Reproduction in the Post-Internet Age: A Contemporary Art + Design Pedagogy
Authors: Berdugo Liat Rachel 
Published: January 2021

Having taught many university-level studio art courses, I have noticed in my students a surprising investment in the notion of originality and authorship in their own creative practices, even as they freely participated in the exchange, reuse, and remixing of visual languages on social media and the internet. This paper focuses on techniques to incorporate remix practices into studio art and design classrooms, arguing that students benefit significantly from a focus on what I term “remix pedagogy,” meaning a method and practice of teaching that brings together both the concepts and the creative use of remix tactics.
I argue that remix pedagogy offers four key benefits to visual arts pedagogy. First, it productively reconfigures outmoded notions of originality and authenticity, freeing students from the high-pressure myth of the artist as a lone genius. In doing so, remix pedagogy offers a second benefit: it introduces students to new modes of making that promote ideation, design thinking, and creative production, including the support of collaborative frameworks in artistic processes. Thirdly—and crucially—these new modes of making teach visual art students how to use artistic practice as social commentary with a cultural fluidity previously unavailable to them. Finally, remix pedagogy familiarizes art and design students with the principles of fair use, promoting complex critical reasoning by forcing them to reason through what makes a work “transformative” and to distinguish fair use from plagiarism. This paper provides examples from my own teaching in Copy Culture, a course I developed at the University of San Francisco.
Pemberdayaan Masyarakat Desa Arjosari di Masa Pandemi: Pengolahan Limbah Cair Tahu menjadi Biogas
Authors: Wulandari Meilinda Ike , Cempakasari Kristina Ela , Anti Nadhia Fikri , Wahyu Ningrum Tri Herbaning Tiyas , Nuraina Elva 
Published: January 2021

Masyarakat Desa Arjosari, rata-rata memiliki penghasilan yang kurang memadai bahkan masih di bawah rata-rata. Disisi lain, di desa tersebut terdapat pabrik tahu Semo UH. Karomah yang limbahnya mencemari lingkungan dan sungai. Sementara limbah tersebut bisa diolah lebih lanjut menjadi suatu produk yang memiliki nilai ekonomis sehingga, adanya dua kondisi tersebut kami tergugah untuk memberdayakan masyarakat melalui pemanfaatan limbah cair tahu menjadi biogas, sehingga dengan pemanfaatan bio gas ini, dapat lebih memberdayakan masyarakat dan dapat menopang kehidupannya sehari hari. Begitu pula untuk para pedagang makanan di Desa Arjosari, pemanfaatan bio gas ini dapat membantu efisiensi biaya pengolahan produknya.Metode yang digunakan dalam pengabdian masyarakat ini adalah Pengabdian masyarakat dalam jaringan (Daring). Mengingat kondisi pandemic corona-19, maka setiap kegiatan dilakukan secara daring. Secara rinci, tahap-tahap pelaksanaan kegiatan ini adalah : 1) Perencanaan ( terdiri dari pembuatan konsep, anggaran, dan jadwal kegiatan) ; 2) Pelaksanaan ( terdiri dari sosialisasi program, pembuatan video animasi, pembuatan e-book, pelatihan pembuatan bio gas secara daring melalui zoom meeting, pembagian file video animasi dan e-book pembuatan bio gas berbahan dasar limbah cair tahu.Berdasarkan aspek social- ekonomi dan pendidikan, pengabdian masyarakat ini memberikan manfaat berupa : 1) Peningkatan pengetahuan masysrakat Desa Arjosari, dalam memanfaatkan limbah cair tahu; 2) Peningkatan skill masyarakat a Desa Arjosari dalam mengolah limbah menjadi produk yang lebih berguna dan bermanfaat; 3) Terwujudnya efisiensi penggunaan bahan bakar 4) Peningkatan taraf ekonomi masyarakat, khususnya para penjual makanan di Desa Arjosari. Proses pembuatan makanan lebih hemat bahan bakar, sehingga lebih efisien; 5)Lingkungan yang bersih dan terhindar dari pencemaran limbah cair tahu.
QUALIDADE DE VIDA RELACIONADA À SAÚDE (HRQL) DE ADULTOS ENTRE 50 E 80 ANOS PRATICANTES DE ATIVIDADE FÍSICA REGULAR: APLICAÇÃO DO SF-36
Authors: Kruger Gonçalves Andréa , Ribeiro Teixeira Adriane , De la Rocha Freitas Cíntia , Jost Blessmann Eliane , Roedel Laysla , Goettert Martins Mariane , Hauser Eduardo , Mattana Griebler Eliane , Copetti Natalia Schardosim , Bós Ângelo José Gonçalves 
Published: January 2021

A qualidade de vida relacionada à saúde-HRQL possui um caráter multidimensional com integração da saúde física, do bem-estar psicológico e da satisfação social. O objetivo foi avaliar a qualidade de vida relacionada à saúde (HRQL) de participantes de meia-idade e de idosos de um projeto de atividade física regular. O tipo de estudo foi descritivo, com corte transversal, sendo que a amostra foi composta por 40 adultos com idade entre 50 e 80 anos, integrantes do CELARI da ESEF/UFRGS. O instrumento utilizado foi o SF-36 e a análise empregada utilizou a estatística descritiva e o teste de correlação de Pearson. A pontuação dos domínios por ordem decrescente foi: AS, AF, AE, EGS, CF, SM, VIT, DOR. Houve correlação positiva significativa entre a maioria dos domínios do SF-36. As correlações não significativas ocorreram entre EGS x AS, SM x AF. A idade apenas correlacionou-se com o domínio EGS. A HRQL da amostra mostrou-se com valores superiores, ao ser comparada com a de outros estudos. Os valores atingiram no mínimo 70% de pontuação, na média dos domínios. Os resultados indicaram um nível satisfatório de HRQL e relação entre os domínios do SF-36. palavras-chave Envelhecimento. Qualidade de Vida. Atividade Física. Saúde. abstract The health-related quality of life – HRQOL is a multidimensional issue with integration of physical health, psychological well-being and social satisfaction factors. The aim of this study was to evaluate the health-related quality of life – HRQOL in ageing people who practice regular physical activity. This study was a descriptive cross-sectional and the sample included 40 adults, aged between 50 and 80 years, attending a University Extension Program. The instrument used was the Brazilian version of Short Form-36. Descriptive statistical analysis was used to summarize HRQL data and Pearson’s correlation for comparison between domains. The score of the domains in descending order were: AS, AF, AE, EGS, CF, SM, VIT, DOR. There was a significant positive correlation between the majority of SF-36. The correlations were not significant between EGS x AS, SM x AF. The age was correlated only with the EGS field. The HRQL of the sample proved to be higher, when compared with other studies. The values ​​reached a minimum score of 70% in average scores. The results indicated a satisfactory level of HRQOL and a significant relationship between different aspects of health-related quality of life. keywords Aging. Quality of Life. Physical Activity. Health.
O IMPACTO CAUSADO PELO LONGO PROCESSO DE MORTE NA FAMÍLIA DO IDOSO ACOMETIDO PELA DOENÇA DE ALZHEIMER.
Authors: Silva Daylene Keyla , Santos Noely Cibeli 
Published: January 2021

Devido ao envelhecimento populacional, a Doença de Alzheimer vem surgindo como um problema causando grande impacto na família. O objetivo do estudo foi compreender a opinião do familiar de idosos com Alzheimer sobre o longo processo de morte. Foi utilizada a abordagem qualitativa. A coleta de dados deu-se através de entrevistas semi-estruturadas, a quatro familiares cuidadores de portadores da doença de Alzheimer. Para a análise dos dados utilizou-se o referencial de análise de conteúdo de Bardin. E aos resultados revelam-se três unidades temáticas: Dificuldades para o cuidar que identificou dificuldades na prática, dentro da família, com o próprio eu, e na primeira perda; Momentos da experiência que identificou as mudanças na vida, as vivências da ausência, a grande confusão de sentimentos e a aproximação do fim; O longo adeus que identificou a despedida de quem se ama e a despedida do corpo de quem ama. Por tanto se pode observar o impacto que o longo processo de morte causa no familiar considerando-se então que devido a enfermagem ter como princípio a humanização, deve cuidar não apenas do doente mas também do familiar, buscando assim aliviar o sofrimento vivenciado por essas pessoas nessa fase da vida.
CARACTERIZAÇÃO FUNCIONAL DE IDOSOS COM ARTRITE REUMATÓIDE
Authors: Fulfaro Mariana De Assis , Zamper Simone Silva e Santos , Luzo Maria Cândida de Miranda , Almeida Maria Helena Morgani de 
Published: January 2021

A artrite reumatóide (AR) é uma das principais causas de incapacidades em idosos, pois suas deformidades geram dificuldades para as atividades cotidianas. Assim, fazem-se necessários estudos que avaliem em que medida ela interfere na capacidade funcional e como essa população minimiza seus efeitos. Com este propósito, foi conduzido estudo em um Hospital Escola da Cidade de São Paulo, em 2008 para caracterização funcional de idosos com esse problema. Participaram da pesquisa 12 pessoas com AR, com pelo menos sessenta anos, que deambulavam, apresentavam comprometimento de mão e punho e estavam em seguimento clínico com reumatologista. Utilizou-se o Mini-Exame do Estado Mental (Folstein, 1975) para inclusão no estudo. Para a caracterização sócio-demográfica e funcional foram utilizados os seguintes instrumentos: Classificação de Idosos quanto à Capacidade para o Autocuidado (Almeida, 2003), Escala Numérica Verbal (Andrade et al, 2006), goniômetro e dinamômetro computadorizados. Os resultados apontam para o comprometimento da capacidade funcional e escassez de respostas de enfrentamento a esta condição. Os participantes apontaram dificuldades para em média 5,6 atividades, sendo que 100% possuía dificuldades físicas para duas ou mais atividades. Em média houve redução da força de preensão palmar e pinça, e da amplitude de movimento. Práticas de autocuidado foram adotadas para somente 36,7% do total de suas atividades diárias. Considerando-se a cronicidade da AR e o próprio envelhecimento, a tendência é que este quadro piore. Dessa forma, evidencia-se a necessidade de serviços de saúde que tenham infraestrutura para manter ou melhorar a capacidade funcional de idosos, visando sua independência e participação social.