bluuebunny/crossref_metadata_2025_split huggingface dataset:
Process design for bioconversion of cassava stalk through solid substrate fermentation
Authors: Dewi P , Indrati R , Millati R , Sardjono  
Published: July 2020

AbstractCassava stalks are by products from cassava tuber production. Cassava stalks is lignocellulosic material (LM) which is composed by lignin, hemicelullose, and cellulose. Utilization of LM optimally will push a beneficial effort in producing sugar. The presence of lignin can limit the effort to convert cassava stalks into sugar. The lignin have to be reduced. Its introduced solid substrate fermentation (SSF) technique by using molds to optimize utilization of cassava stalks. Molds can produce enzyme for converting LM into sugar. There is no enough information of design process of solid state fermentation for cassava stalks bioconversion, starting from selection of suitable microorganism used, substrate suitability testing, lignin reducing pretreatment, design of fermentor, and the needed of aeration for optimizing molds growth. Based on research result, a process for bioconversion of cassava stalks into sugar through SSF techniques was defined as follow: Microorganism suitable used isAspergillus niger, cassava stalks suitable as a growth media for mold as a physical support (DMC = 93, 76 %) and source of nutrient (C/N 19, 92), physical pretreatment by reduce the particle size was more suitable than alkaline pretreatment for lignin reduction, and the best fermentor is unforced aeration closed chamber at big capacity.
Bioactive compounds from callus culture of Elaeocarpus grandiflorus
Authors: Habibah N A , Nugrahaningsih WH , Musafa F , Rostriana Y , Mukhtar K , Wijawati N , Anggraito Y U 
Published: July 2020

Abstract

E. grandiflorus has potential as a source of bioactive compounds. This study aims to analyze the content of flavonoid and phenolic bioactive compounds in the callus culture of E. grandiflorus in various concentrations of PGR. Callus culture induction was carried out by maintaining E. grandiflorus leaf stalk explants on solid Murashige & Skoog (MS) with the addition of growth regulators namely 2,4-dichlorophenoxyacetic acid (2,4-D) and picloram in different concentrations. The results of the study showed that callus maintained on MS medium with the addition of 2,4-D and picloram could produce flavonoids and phenolics. Flavonoid and phenolic concentrations produced in each treatment varied. Overall, it can be concluded that the MS medium with additional growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) or picloram can be used for bioactive compound production of callus Elaeocarpus grandiflorus.
The use of two pairs primer for CO1 gene amplification on traded stingray at fish auction Tasik Agung Rembang
Authors: Setiati N , Partaya  , Hidayah N 
Published: July 2020

Abstract
Stingray provide a high economic contribution and increase in production and utilization. Conservation effort needed to identify molecularly using CO1 gene. The success of CO1 gene in identifying species can not be separated from amplification in polymerase chain reaction (PCR) technique. The use of primer FishF1, FishR1, FishF2, and FishR2 to amplify COI gene for stingray traded at fish auction Tasik Agung Rembang never been done, so it need to be researched. The purpose of this study is to amplify the COI gene of stingray traded at fish auction Tasik Agung Rembang using two pairs of primers. Five stingrays sample were obtained from fish auction Tasik Agung Rembang. Then its DNA was isolated using protocol from TIANamp Marine Animals DNA Kit and measured in quantity using nano spectrophotometer. Amplification of CO1 gene by PCR technique. Based on the results of the study showed that the five samples had good DNA purity, concentration, and quality. The 5 samples amplification result showed that the FishF1-FishR1 primer pair succeeded in amplifying samples a, b, d, and e, while the FishF2-FishR2 primer pair succeeded in amplifying samples b, c, d, and e with a PCR product length around 655 bp.
The use of eggshell powder as calcium source in stabilizing expansive soil using Bacillus subtilis
Authors: Sugata M , Widjajakusuma J , Augestasia A , Zacharia A , Tan T J 
Published: July 2020

Abstract
Expansive soils are commonly characterized by low strength and high swelling potential. Thus, to use this type of soil as foundation, stabilization process is needed. This research aimed to improve the stability of expansive soils by using Bacillus subtilis. The bacteria have been reported for its ability to form calcium carbonate precipitation which could bind soils particles, hence, increase the soil strength. In this study, the bacteria were grown in medium containing eggshell as calcium source. Then, the bacterial liquid culture was injected into expansive soil samples, followed by curing period of 30, 60 and 90 days. The swelling potential of the stabilized soil samples were evaluated with free swell index (FSI) test. Meanwhile, the soil strength was analysed with unconfined compressive strength (UCS) as well as direct shear (DS) tests. The results showed that Bacillus subtilis could use eggshell as calcium source to improve the stability of expansive soils. The use of higher bacterial cell concentration and longer curing period showed higher improvement in soil strength. After 90 days of curing period, there was a 30% decrease in the FSI, 74.32% increase in the UCS, and 77.27% increase in the cohesion of soil samples.
Identification of ATPase6 gene mutation from cimahi clinical isolates
Authors: Satiyarti R B , Ramadhan R , Mulyani R 
Published: July 2020

Abstract
Diabetes mellitus is a mitochondrial disease, caused by ATP deficiency. ATP was produced by the OXPHOS system in the mitochondria. Mitochondrial ATPase6 was one of important enzyme in oxidative phosphorylation process of ATP synthesis. The aim of this research was to provide information of ATP6 gene mutation that correlated to DMT2. A pair of primers was designed by in silico study. Blood samples were taken from DMT2 patients. Blood cells were lysed to obtain DNA template. Amplification of ATP6 gene was done by Polymerase Chain Reaction (PCR) technique. The amplicon was analyzed by 1% agarose gel electrophoresis. The gel showed 0,7 kb band of amplicon. The nucleotide sequencing showed that the amplicon was 681 base pairs. Analysis of phylogenetic showed that the sequence was 94,08% identical to homo sapiens ATP6 gene. Homology analysis between ATP6 gene from genbank and ATP6 gene fragments showed that there was a mutation 8860A>G. Amino acid analysis showed that 8860A>G change the amino acid T112A. Interestingly. It both happened in all samples, and it is a haplogroup. This is mean, it need further research to convince 8860A>G related to DMT2.
The allele frequency of C825T GNB3 gene in hypotension
Authors: Nugrahaningsih W H , Susanti I , Susanti R , Anggaraito Y U 
Published: July 2020

Abstract
Hypotension is signed by the systolic blood pressure less than 90 mmHg and the diastolic less than 60 mmHg. The prevalence of orthostatic hypotension is high, about 10-33%. Genetic polymorphism one of factors influenced the prevalence of blood pressure abnormality. GNB3 gene located at chromosome 12p13 influenced blood pressure by their involved in the second messenger during the transduction pathway. The survey conducted to 54 volunteers (29 normotension and 25 hypotension). The peripheral blood was collected from brachial vein. GNB3 gene were analysis by PCR-RLFP. DNA extraction was processing by GeneJET Genomic DNA Purification Kit. The genotype frequency of CC: CT:TT was 12%:72%:16% in hypotension, while 21%:52%:17% in normotension. The allele frequency of C:T was 48:52% in hypotension and 46,5:53,5% in normotension. The frequency allele of C and T were difference between hypotension and normotension (p=0.005). Concluded that C825T of GNB3 gene associated with hypotension evidence.
Comparison and characteristics of antioxidant components of traditional herbal medicine hygienically produced
Authors: Rudyatmi E , Bintari S H 
Published: July 2020

AbstractAntioxidants in traditional herbal medicine are proven to have health benefits. The purpose of this study were to compare and characterize the antioxidant components of traditional herbal medicines serbat secang, keras kencur, kunyit asam and zingiber curcuma (zicurma). The total antioxidants of phenols, flavonoids, tannins, alkaloids and saponins were determined with a spectrophotometry, viscosity with a viscometery, and coloform with coliform test. Total phenols, flavonoids, tannins, alkaloids and saponins as well as viscosity on T: 67,25; 19.36; 114.52; not detected; 0.06; and 3.45. BK: 16,19; 30.07; 21.90; 0.03; 0.09; and 5.85. KA: 41.06; 27.31; 39.58; not detected; 0.13 and 5.10. Zicurma is made fromCurcuma xanthorrhiza,Curcuma longa, andZingiber officinallecontain the highest antioxidants, namely: total phenol 91.62 mg; flavonoids 127, 50; tannin 119.70 mg; alkaloid 0.003%; and 0.58% saponins. Viscosity 15.30 cP. Coliform on all herbs were not detected. In conclusion, antioxidants content on Sc, BK, KA, and zicurma are varies. Viscosity is ideal asa a drink and hygienically produced. Zicurma is a potential traditional herbal medicine that has higher antioxidant component than others. Traditional herbal medicine is very potential, hygienic and suitable for consumption every day.
The findings of character values are integrated in the mathematics learning process (case study in Guangxi Normal University)
Authors: Rochmad  , Suyitno H , Sugiharti E , Yuanbing G 
Published: July 2020

Abstract
Good character values need to be possessed by students at the University. Based on the Regulation of Rector, No. 19 Year 2016, UNNES has 12 Ethics, namely: science for truth, academic freedom, harmony, responsibility, honesty, justice, caring, exemplary, discipline, politeness, non-discrimination, and mutual respect. China is known to have a good character education from elementary school to university level. So, we need a collaboration work with a university in China, namely: Guangxi Normal University (GXNU). Partner Lecturer: Prof. Dr. Guo Yuanbing. The problem: What character values are integrated into the learning process of mathematics at GXNU? The method of research: Qualitative approach. The research subjects of Local Students of GXNU were selected. The main activities in GXNU: Observation, interviews, triangulation, and Focus Group Discussion (FGD) in GXNU, guided by Prof. Dr. Guo Yuanbing. The results (1) The character values that found and grown in GXNU were as follows: Prosperity, Democracy, Civility, Harmony, Freedom, Equality, Justice, Rule of Law, Patriotism, Dedication, Integrity, and Friendship. (2) Lecturer of Mathematics Education of GXNU was given the freedom to apply some or all of the values of character into teaching and learning activities; Character values were not explicitly added into the Lesson Plan of a Math lecturer in GXNU; Violations of the application of character values were left entirely to the lecturer concerned with the support of the institution.
Mechanical strength of quartz reinforced polyvinyl acetate/leaves-waste composite
Authors: Masturi  , Alighiri D , Susilawati  , Sunarno  
Published: July 2020

Abstract
A quartz reinforced polyvinyl acetate/leaves-waste composite has been made using simple pressing method. This work was performed as an answer for the solid waste abundance and deforestation activity. Having been wet-milled using ethanol 70% and dried for 4 days, the quartz was added as reinforcement in polyvinyl acetate (PVAc)/leaves waste mixture with varied contents to form PVAc/leaves waste/quartz composite via pressing at 5 metric ton for 20 minutes. The compressive strength test shows the mechanical strength varies from 35.53 to 39.93 MPA as quartz contents, with a sample having quartz sand content 4.60 × 10−3 (w/w) has maximum mechanical strength as 39.93 MPa, and the addition of quartz above the content makes the mechanical strength tends to decrease. The strength values are comparable to some previous works by several authors and some building materials, such Sago pulp composite, polyurethane/solid waste/nanosilica composite, agriculture-waste/silica composite, soil brick, limestone, natural fiber reinforced concrete and Portland cement concrete. This result shows the quartz reinforced polyvinyl acetate/leaves-waste composite has been acceptable to compete the woods as building materials to reduce deforestation.
Problem-solving ability using mathematical modeling strategy on model eliciting activities based on mathematics self-concept
Authors: Kharisudin I , Cahyati N E 
Published: July 2020

Abstract
The purpose of this study is to test the effectiveness of problem-solving skills using mathematical modeling strategy on MEAs and to describe problem-solving skills based on the mathematics self-concept. This research uses a quantitative method followed by descriptive with research variables are problem-solving ability and mathematics self-concept. Data collection techniques used in this study are documentation, tests, questionnaires, and interviews, then processed with a classical completeness test, t-test, proportion test, and linear regression test. We find that the ability of problem-solving with mathematical modeling strategy on Model Eliciting Activities achieved completeness. The ability to solve problems with mathematical modeling strategies on Model Eliciting Activities is better than conventional learning models. Student’s mathematics self-concept has a positive effect on problem-solving ability by 31.2%. There are two subjects with high mathematics self-concept categories have high and low problem-solving skills, and two subjects with medium and low mathematics self-concept groups have medium and low problem-solving skills.
Microgravity method to monitor subsidence in Kota Lama area Semarang
Authors: Supriyadi  , Khumaedi  , Sugiyanto  , Heparona Jefta 
Published: July 2020

Abstract
The purpose of this study was to monitor the subsidence that occurred in the Kota Lama. The method used is the micro gravity method. Measurements were carried out 2 times in the period May and September 2017. The anomalous data obtained time lapse microgravity anomaly which were the weight difference between the period of September and May. This anomalous data still contains anomalous sources of groundwater leve changes which are then corrected with rainfall data for that time interval. Finally anomaly data were obtained which were the target of the study in the form of microgravity anomaly data due to subsidence. After being converted with a constant free air correction resulted in a maximum subsidence of 0.3 cm or 3 mm occurred in the north and south of the Kota Lama.
Non-destructive test of watermark materials quality for art pottery using x-ray digital radiography
Authors: Dewi N R , Listiaji P , Marwoto P , Iswari R S 
Published: July 2020

Abstract
In this study, the quality of watermark materials for art pottery were tested using x-ray digital radiography. The embedded watermarks in art pottery were chosen from 3 materials namely wood, and polylactic acid (PLA), and acrylic. Watermarked potteries quality testing were carried out during the making process, namely before and after being burned. Watermarked potteries were tested with 60 kV tube voltage regulation, 16 mA strong current, and 0.16 s exposure time. The radiographic image results obtained were then analyzed using ImageJ software. There are 3 stages of testing, namely visual analysis, image line profile analysis, and linear attenuation coefficients analysis. The results of visual analysis show that there were no crack in the watermarks so that planting the watermark did not damage the pottery. The graph of image line profile analysis results shows that the watermark made from wood was most clearly seen on the radiographic image. The attenuation coefficient value in the watermarked part does not change before and after the burning process while the attenuation coefficient value of the pottery had increased due to the shrinking process of the pottery. Wood has the largest attenuation coefficient value difference with pottery, so making it suitable as a watermark material for art pottery.
The effect of problem based learning supported by computer simulation student’s creative thinking skills
Authors: Simajuntak M P , Marpaung N , Barus P , Lestari R I , Nova B , Ompusunggu E 
Published: July 2020

Abstract
Generally, student’s have low score of creative thinking skills. The students who have a low creative thinking skills will be difficult, in solve the problem. Creative thinking skills will increase learning process. This research aims to determine the effect of PBL with computer simulation towards student’s creative thinking skills. The research method use a pretest-posttest design. The subject of this research are three classes, with experiment1 class simulation supported with computer simulations used PBL, PBL use the experiment2 class, and the contol class use conventional learning. The instrument use essay tests that measure creative thinking skills based on problem. The avarage of pretest and posttest in experiment1 class are 20.28 and 76.62 with N-gain of 71%, in experiment2 class 20,84 and 68,06 with N-gain of 60%, and in the control class 20,35 and 44,05 with N-gain of 30% and all of that classes in the medium category. Based on the results of testing that there is significant difference obtained on PBL applied supported with computer simulations towards student’s creative thinking skills.
Profile of senior high school students’ misconception in physics using need-based analysis
Authors: Trisniarti M D , Aminah N S , Sarwanto S 
Published: July 2020

Abstract
This study obtained the current situation of physics teaching, learning, and assessment at the senior high school in Surakarta, Indonesia based on various skills in 21st century. A need-based analysis was conducted to explore students’ current situation that lead to misconception in understanding concept of physics as learning objectives. Through random sampling, students’ questionnaires and teachers questionnaires to over 120 students and 12 teachers were held respectively. The implications showed that generic science skill and interpersonal skills had influenced students’ understanding of physics as the two affecting factors could be the reasons for misconception. From the questionnaires result, it showed that teachers and students of senior high school in Surakarta need a proper diagnostic test to investigate and map students’ misconception in physics classroom especially in difficult learning object such as kinetic gas theory. The four-tier diagnostic test with five possible answers was developed based on the result of need-based analysis and factors affecting students’ misconception which were students’ generic science and interpersonal skills.
Measurement of critical thinking in physics: the identification of students’ critical thinking skill through the work report on momentum conservation?
Authors: Saputri D I , Sunarno W , Supriyanto A 
Published: July 2020

Abstract
This study aims to measure students’ critical thinking skills through the ability to make practical work reports. The method used is qualitative descriptive method in the form of critical thinking skills through assessment of student work reports and questionnaire data. Rubric that used to measure students’ critical thinking skills with 10 indicators: clarity, accuracy, precision, consistency, relevance, strength, reason, depth, breadth and fairness. The study sample was 34 students. The results showed that students’ critical thinking skills through work reports were still 17.6% of students already had very high critical thinking skills, 58.82% had moderate critical thinking skills and as many as 23.53% students have weak critical thinking skills. Students have a high category in the breadth. Then clarity, accuracy, precision, consistency, and relevance have a moderate and evidence has a weak. The results of the student and teacher questionnaires about the application of practicum also stated that before practicum students were still sometimes given time to conduct preliminary studies.
Students’ responses on the development of flash learning media in light material
Authors: Ahied M , Wulandari A Y R , Munawaroh F , Rosidi I 
Published: July 2020

Abstract
This research aim was to know the student responses on the development of flash learning media in light material. The method of developing learning media used ADDIE which included 5 stages, namely Analysis, Design, Development, Implementation, and Evaluation. The instrument used to measure student responses to learning media used questionnaires. The sample used to retrieve student responses were 36 students. Analysis of student responses used descriptive statistics. Based on the results of data analysis, it can be seen that student interest in learning media was 85.94% with a very good category, students’ conceptual understanding was 80.82% with very good categories, and assessment of the appearance of learning media was 77, 22% with good categories. From these results, it can be concluded that the student responses to the media developed was very good with an average percentage of 81.33%.
How are student’s cognitive patterns viewed from higher-order thinking skills in kinematics?
Authors: Hasanah T A N , Handayanto S K , Zulaikah S , Yuenyong C 
Published: July 2020

Abstract
Higher-Order Thinking Skills (HOTS) is an ability that must be achieved by students today because this ability is the core of assessment in K13 and also as demand for 21st-century skills. Giving HOTS-based tests is an attempt to explore further the extent to which HOTS is mastered by students in kinematics and also describe cognitive patterns based on student’s HOTS. The method used in this study is descriptive, with an in-depth analysis of student answers. The research subjects were 51 students. The test instrument used consisted of five HOTS questions, which were divided into two analyzing questions, 1 evaluating question and 2 creating questions, has a reliability coefficient of 0.85 with considered high. The results of the study show that the cognitive patterns of students with high HOTS tend to be stable increasing following cognitive levels, for cognitive patterns of students with medium HOTS are having an up and down pattern, while the cognitive patterns of students with low HOTS tend to decline at higher cognitive levels. The whole HOTS in this study is relatively low because only 3.92% of students can reach high HOTS. Thus, it is necessary to apply of learning model such as Problem Based Learning to train students for higher order thinking in solving physics problems.