bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unusual Presentation of an Esophagopleural Fistula in a Patient With Severe Esophagitis: Blunt Trauma or Iatrogenic?
Authors: Wang Yanting , Rivas Chicas Oscar A. , Basu Anupam 
Published: November 2019

ABSTRACT
Esophagopleural fistula (EPF) is rare despite the anatomical proximity of the esophagus and the pleural space. A 64-year-old man presented with a pneumothorax after a fall requiring chest tube placement. An esophagogastroduodenoscopy revealed severe LA grade D esophagitis and a large EPF in the distal esophagus. Computed tomography scan revealed that subtle tracking of air extending from the distal esophagus into the right pleural space was noted. The patient was treated with placement of a fully covered esophageal metal stent, and he recovered uneventfully. Interpreting key subtle clues in pleural fluid analysis and imaging can lead to a timely diagnosis and thus improves morbidity and mortality of EPF.
Inquiry at the Botanical Gardens: Practicing Science Processes during a Field Trip to the <Blinded> Zoo
Authors: McConnell Tom J. , Foster Tolly , Schilten Michele 
Published: November 2019

Field trips offer excellent learning opportunities, but teachers often think of them as informal experiences to teach concepts and content. But it is possible to structure science lessons at sites like zoos, aquariums and museums as an inquiry activity. Using the NRC (2000) definition of inquiry and the Indiana Academic Standards list of process standards (IDOE 2016), facilitators at the BLINDED ACADEMY developed the Plant Adaptations Inquiry lesson. This activity lets learners hypothesize, observe, analyze data and construct conclusions about the types of plant adaptations seen in plants from different ecosystems. The observations, including the use of technology to use photographs, were recorded on the grounds of the BLINDED Gardens and the BLINDED Zoo. This article explains the design of the lesson and alignment with state and national standards. Readers can download a version of the lesson and rubric.
Perbedaan Metode Mutilasi Terhadap Lama Waktu Molting Scylla serrata
Authors: Ario Raden , Djunaedi Ali , Pratikto Ibnu , Subardjo Petrus , Farida Fauzia 
Published: November 2019

Kepiting bakau (Scylla serrata) memiliki nilai ekonomis tinggi. Kebutuhan kepiting bakau selalu meningkat sehingga perlu diupayakan budidaya kepiting bakau secara intensif. Salah satu perkembangan teknologi dalam budidaya perikanan untuk meningkatkan produksi kepiting bakau adalah produksi kepiting cangkang lunak. Kepiting cangkang lunak merupakan kepiting fase ganti kulit (molting) yang mempunyai keunggulan cangkangnya lunak sehingga dapat dikonsumsi secara utuh. Untuk mempercepat kepiting molting diperlukan berbagai rangsangan yang salah satunya adalah menggunakan metode mutilasi. Tujuan penelitian untuk mengetahui perbedaan lama waktu molting dan pertumbuhan berat kepiting bakau dengan menggunakan metode mutilasi pada kaki jalan dan capit. Metode penelitian yang digunakan yaitu metode eksperimental dengan rancangan acak lengkap (RAL) yang terdiri dari empat perlakuan, yaitu mutilasi kaki jalan dan capit, semua kaki jalan, capit, dan alami. Biota yang digunakan berjumlah 40 ekor dengan 10 kali ulangan tiap perlakuan. Data yang diperoleh berupa lama waktu molting serta pertambahan berat mutlak kepiting bakau yang dianalisis menggunakan uji statistik parametrik. Hasil penelitian menunjukkan metode mutilasi berpengaruh terhadap lama waktu molting dengan waktu molting tercepat pada perlakuan mutilasi kaki jalan dan capit rata-rata 13 hari. Metode mutilasi tidak berpengaruh terhadap pertumbuhan berat mutlak dengan nilai tertinggi pada kepiting perlakuan alami sebesar 53,30 gram. Mud crabs (Scylla serrata) are known to have a high economic value. The increasing demand of mud crabs for consumption rxcequires higher production. Therefore, mud crabs need to be cultivated intensively. One of the methods to improve the values of mud crabs’ aquaculture is by producing soft-shell crabs. Soft-shell crabs are produced during molting phase in which the crab shed it’s exoskeleton in order to grow. In the fisheries industry, the soft-shell crabs are considered to be more valuable as it can be consumed as a whole. Accelerating the production of molting crabs, requires stimulus. One of the methods is mutilation. The aim of this study is to estimate the periods required for molting under different treatments, as well as calculating the increase of total weight of molting crabs.. The method used was an experimental method which contained four treatments. The treatments are mutilation of walking legs and claws, all of walking legs, claws, and no mutilation. The number of crabs used was 40 with 10 replications per treatment. The data obtained in the period of molting and the increase of total weight of the mud crabs were analyzed using ANOVA. The result shows that mutilation affects the period of crab’s molting in which the fastest molting (13 days on average) occurred after mutilation of walking legs and claws. This mutilation method does not influence the increase of total weight and the highest value is showed in non-treated group with the increase of 53,30 grams in weight.
Precision medicine applications for severe asthma
Authors: Hacohen Orit Gourgy , Cohen Shai 
Published: November 2019

Asthma is a heterogeneous condition in which multiple pathological pathways manifest with similar symptoms. Severe asthma (SA) is challenging to manage and comprises a significant health and economic burden. Many studies have been conducted in an attempt to define different clinical phenotypes that translate into biological endotypes, with the goal of tailoring treatment based on precision medicine. This review summarizes the current evidence for the treatments of SA, and in particular, the biologic treatments that are currently available: omalizumab, mepolizumab, reslizumab, benralizumab and dupilumab. We found only limited high-quality direct evidence regarding treatment with anti-IgE (omalizumab) in SA patients. Data regarding anti-interleukin (IL)-5 (mepolizumab, reslizumab and benralizumab) showed beneficial effects in severe eosinophilic asthma (SEA) with different levels of blood eosinophils used in clinical trials. Dupilumab, anti-IL-4/IL-13, was shown to be effective in SEA and is the only agent currently FDA-approved for the indication of oral corticosteroid dependent asthma, regardless of the blood eosinophil level. This review also summarizes the existing knowledge regarding the characteristics of the patient who may respond to the different therapies. As of today, more studies are needed to better understand the diverse mechanisms that underlie SA phenotypes. We have not yet adequately reached the goal of precision medicine. Additional studies are necessary in order to find novel surrogate markers that can predict the response to a specific biologic therapy, especially in patients who are oral corticosteroid dependent. In addition, efforts must be invested into research looking for new treatment options for patients with non-type-2 inflammation SA. Statement of novelty: we review the current evidence regarding tailored treatment therapies in SA, with a particular focus on the knowledge regarding patient selection for specific biologic treatments.
Elements of Stars
Authors: Lugaro Maria , Dishoeck Ewine van 
Published: November 2019

Abstract
What are stars made of? Less than 200 years ago this basic, simple question was deemed impossible to answer. As Auguste Comte put it in 1835: “On the subject of stars…While we can conceive of the possibility of determining their shapes, their sizes, and their motions, we shall never be able by any means to study their chemical composition or their mineralogical structure.” Today, we have a broad, clear answer to the question: “What are stars made of?” We also understand its far-reaching implications in relation to the evolution of the cosmos. Satellites and many ground-based spectroscopic surveys routinely provide new discoveries on the chemical composition of astronomical objects. In parallel, the nuclear processes that produce the elements inside stars are investigated in increasingly sophisticated nuclear physics experimental facilities across the world. At the same time, supercomputers allow us to calculate detailed models of the evolution of stars and galaxies: how much of which element is produced where? Finally, the presence of tiny amounts of extra-solar material can be found within meteorites, whose analysis is reaching unparalleled precisions with uncertainties down to parts per million. How have we managed to travel from an impossible question to such broad knowledge filled with discoveries?
Elements of Technology
Authors: Droescher Michael 
Published: November 2019

Abstract
There are many ways to look at the periodic table. When Mendeleev established the table, he arranged the elements according to atomic number and chemical properties. Here, we try a different look. We discuss the influence and links of elements on the development of cultures and technologies. Some of the elements have a direct impact on our life: carbon, nitrogen, hydrogen, oxygen and phosphorous are the main building blocks of our organism, besides small amounts of other elements. Many other elements, especially metals, are the base of the development of our social and technological world, due to the availability and/or innovative use of new or already known elements. We will have a look on the relevance and importance of these elements for the development of technologies over the last 10 millennia.
In Memoriam: René Dybkaer
Authors: Hibbert Brynn 
Published: November 2019

Abstract
I first met René when the IUPAC General Assembly came to Brisbane Australia in 2001. IUPAC was not an organization I (then) knew much about, but I found myself confronted by Paul De Bièvre demanding that I join a task group on metrological traceability. At the time I would not have called myself a metrologist in chemistry (MiC) or in any other field. The great minds assembled for the project were Paul De Bièvre, René Dybkaer, Ales Fajgelj, and me. I had no idea that our task would take ten years (finally published as De Bièvre, P.; Dybkaer, R.; Fajgelj, A.; Hibbert, D. B.: Metrological traceability of measurement results in chemistry: Concepts and implementation (IUPAC Technical report) Pure Appl. Chem. 2011, 83, 1873-1935), and that during the time I would come to form strong friendships with my comrades in the “Gang of Four.” Paul is no longer with us and now René has left the stage.
The application of a phosphorus nitrogen flame retardant curing agent in epoxy resin
Authors: Li Anxin , Mao Pingli , Liang Bing 
Published: November 2019

AbstractIn order to improve the compatibility of flame retardant and epoxy resin, a phosphorus nitrogen flame retardant curing agent poly(p-xylylenediamine spirocyclic pentaerythritol bisphosphonate) (PPXSPB) was synthesized. FTIR,1HNMR, and mass spectroscopy were used to identify the chemical structure of PPXSPB. Epoxy resin (E-44) and PPXSPB as the raw material, a series of thermosetting systems were prepared. The effects of PPXSPB on flame retardancy, water resistance, thermal degradation behavior, mechanical properties and the adhesive strength of EP/PPXSPB thermosets were investigated. The results show that with the increase of phosphorus content, the oxygen index and carbon residue of the system both increased significantly, and the heat release rate gradually decreased, which is of great significance in delaying the occurrence of fire. When the phosphorus content is 3.24% in EP/PPXSPB thermosets, EP-2 can successfully pass the UL94 V-0 flammability rating, the LOI value of EP-2 can reach 31.4%, the impact strength and tensile strength was 6.58 kJ/m2and 47.10 MPa respectively, and the adhesive strength was 13.79 MPa, the system presents a good overall performance.
Effect of salt and temperature on molecular aggregation behavior of acrylamide polymer
Authors: Ma Jingyuan , Yu Peizhi , Xia Boru , An Yuxiu 
Published: November 2019

AbstractThree kinds of acrylamide copolymers were synthesized in this paper, acrylamide (AM) homopolymer, acrylamide/acrylic acid (AA) copolymer and acrylamide/2-acrylamide-2-methylpropanesulfonic acid (AMPS) copolymer by free radical polymerization. The aggregation behaviours of these polymers in salts water solution were studied. The molecular chains of homopolymer were stretched in salt water solutions, since it did not undergo hydrolysis and there were no charges in the backbone and side chains. Therefore, the conformation of homopolymer was hard to be attacked by salt ions. However, the copolymers synthesized from these monomers had large number of anionic groups. These anions were easily attracted by cations from salt solutions forming ionic bonds, which led to changes in molecular conformation. The viscosity of copolymer decreased since the molecular chains were crimped. The viscosity of copolymer decreased distinctly according to the temperature of different salt solutions, as temperature had great impact on the viscosity of copolymer. The interaction mechanism of polymers exhibited great difference in salts solutions, comparing to temperature. High temperature reduced the entanglement between molecular chains, whereas salt ions neutralized the negative charges on the molecular chains causing curling of molecular chains.
Long-Term Exposure to PFE-360 in the AAV-α-Synuclein Rat Model: Findings and Implications
Authors: Andersen Michael Aagaard , Sotty Florence , Jensen Poul Henning , Badolo Lassina , Jeggo Ross , Smith Garrick Paul , Christensen Kenneth Vielsted 
Published: November 2019

AbstractParkinson’s disease (PD) is a progressive neurodegenerative disorder associated with impaired motor function and several non-motor symptoms, with no available disease modifying treatment. Intracellular accumulation of pathological α-synuclein inclusions is a hallmark of idiopathic PD, whereas, dominant mutations in leucine-rich repeat kinase 2 (LRRK2) are associated with familial PD that is clinically indistinguishable from idiopathic PD. Recent evidence supports the hypothesis that an increase in LRRK2 kinase activity is associated with the development of not only familial LRRK2 PD, but also idiopathic PD. Previous reports have shown preclinical effects of LRRK2 modulation on α-synuclein-induced neuropathology. Increased subthalamic nucleus (STN) burst firing in preclinical neurotoxin models and PD patients is hypothesized to be causally involved in the development of the motor deficit in PD. To study a potential pathophysiological relationship between α-synuclein pathology and LRRK2 kinase activity in PD, we investigated the effect of chronic LRRK2 inhibition in an AAV-α-synuclein overexpression rat model. In this study, we report that chronic LRRK2 inhibition using PFE-360 only induced a marginal effect on motor function. In addition, the aberrant STN burst firing and associated neurodegenerative processes induced by α-synuclein overexpression model remained unaffected by chronic LRRK2 inhibition. Our findings do not strongly support LRRK2 inhibition for the treatment of PD. Therefore, the reported beneficial effects of LRRK2 inhibition in similar α-synuclein overexpression rodent models must be considered with prudence and additional studies are warranted in alternative α-synuclein-based models.
Neurons in the Intermediate Reticular Nucleus Coordinate Postinspiratory Activity, Swallowing, and Respiratory-Sympathetic Coupling in the Rat
Authors: Toor Rahat Ul Ain Summan , Sun Qi-Jian , Kumar Natasha N. , Le Sheng , Hildreth Cara M. , Phillips Jacqueline K. , McMullan Simon 
Published: November 2019

Breathing results from sequential recruitment of muscles in the expiratory, inspiratory, and postinspiratory (post-I) phases of the respiratory cycle. Here we investigate whether neurons in the medullary intermediate reticular nucleus (IRt) are components of a central pattern generator (CPG) that generates post-I activity in laryngeal adductors and vasomotor sympathetic nerves and interacts with other members of the central respiratory network to terminate inspiration. We first identified the region of the (male) rat IRt that contains the highest density of lightly cholinergic neurons, many of which are glutamatergic, which aligns well with the putative postinspiratory complex in the mouse (Anderson et al., 2016). Acute bilateral inhibition of this region reduced the amplitudes of post-I vagal and sympathetic nerve activities. However, although associated with reduced expiratory duration and increased respiratory frequency, IRt inhibition did not affect inspiratory duration or abolish the recruitment of post-I activity during acute hypoxemia as predicted. Rather than representing an independent CPG for post-I activity, we hypothesized that IRt neurons may instead function as a relay that distributes post-I activity generated elsewhere, and wondered whether they could be a site of integration for para-respiratory CPGs that drive the same outputs. Consistent with this idea, IRt inhibition blocked rhythmic motor and autonomic components of fictive swallow but not swallow-related apnea. Our data support a role for IRt neurons in the transmission of post-I and swallowing activity to motor and sympathetic outputs, but suggest that other mechanisms also contribute to the generation of post-I activity.SIGNIFICANCE STATEMENTInteractions between multiple coupled oscillators underlie a three-part respiratory cycle composed from inspiratory, postinspiratory (post-I), and late-expiratory phases. Central post-I activity terminates inspiration and activates laryngeal motoneurons. We investigate whether neurons in the intermediate reticular nucleus (IRt) form the central pattern generator (CPG) responsible for post-I activity. We confirm that IRt activity contributes to post-I motor and autonomic outputs, and find that IRt neurons are necessary for activation of the same outputs during swallow, but that they are not required for termination of inspiration or recruitment of post-I activity during hypoxemia. We conclude that this population may not represent a distinct CPG, but instead may function as a premotor relay that integrates activity generated by diverse respiratory and nonrespiratory CPGs.
Audiogenic Seizures in theFmr1Knock-Out Mouse Are Induced byFmr1Deletion in Subcortical, VGlut2-Expressing Excitatory Neurons and Require Deletion in the Inferior Colliculus
Authors: Gonzalez Darya , Tomasek Madison , Hays Seth , Sridhar Vinay , Ammanuel Simon , Chang Chia-wei , Pawlowski Karen , Huber Kimberly M. , Gibson Jay R. 
Published: November 2019

Fragile X syndrome (FXS) is the most common form of inherited intellectual disability and the leading monogenetic cause of autism. One symptom of FXS and autism is sensory hypersensitivity (also called sensory over-responsivity). Perhaps related to this, the audiogenic seizure (AGS) is arguably the most robust behavioral phenotype in the FXS mouse model—theFmr1knock-out (KO) mouse. Therefore, the AGS may be considered a mouse model of sensory hypersensitivity. Hyperactive circuits are hypothesized to underlie dysfunction in a number of brain regions in patients with FXS andFmr1KO mice, and the AGS may be a result of this. But the specific cell types and brain regions underlying AGSs in theFmr1KO are unknown. We used conditional deletion or expression ofFmr1in different cell populations to determine whetherFmr1deletion in those cells was sufficient or necessary, respectively, for the AGS phenotype in males. Our data indicate thatFmr1deletion in glutamatergic neurons that express vesicular glutamate transporter 2 (VGlut2) and are located in subcortical brain regions is sufficient and necessary to cause AGSs. Furthermore, the deletion ofFmr1in glutamatergic neurons of the inferior colliculus is necessary for AGSs. When we demonstrate necessity, we show thatFmr1expression in either the larger population of VGlut2-expressing glutamatergic neurons or the smaller population of inferior collicular glutamatergic neurons—in an otherwiseFmr1KO mouse—eliminates AGSs. Therefore, targeting these neuronal populations in FXS and autism may be part of a therapeutic strategy to alleviate sensory hypersensitivity.SIGNIFICANCE STATEMENTSensory hypersensitivity in fragile X syndrome (FXS) and autism patients significantly interferes with quality of life. Audiogenic seizures (AGSs) are arguably the most robust behavioral phenotype in the FXS mouse model—theFmr1knockout—and may be considered a model of sensory hypersensitivity in FXS. We provide the clearest and most precise genetic evidence to date for the cell types and brain regions involved in causing AGSs in theFmr1knockout and, more broadly, for any mouse mutant. The expression ofFmr1in these same cell types in an otherwiseFmr1knockout eliminates AGSs indicating possible cellular targets for alleviating sensory hypersensitivity in FXS and other forms of autism.
Developmental Pathogenicity of 4-Repeat Human Tau Is Lost with the P301L Mutation in Genetically Matched Tau-Transgenic Mice
Authors: Gamache Julia E. , Kemper Lisa , Steuer Elizabeth , Leinonen-Wright Kailee , Choquette Jessica M. , Hlynialuk Chris , Benzow Kellie , Vossel Keith A. , Xia Weiming , Koob Michael D. , Ashe Karen H. 
Published: November 2019

Tau is a microtubule-associated protein that becomes dysregulated in a group of neurodegenerative diseases called tauopathies. Differential tau isoforms, expression levels, promoters, and disruption of endogenous genes in transgenic mouse models of tauopathy make it difficult to draw definitive conclusions about the biological role of tau in these models. We addressed this shortcoming by characterizing the molecular and cognitive phenotypes associated with the pathogenic P301L tau mutation (rT2 mice) in relation to a genetically matched transgenic mouse overexpressing nonmutant (NM) 4-repeat (4R) human tau (rT1 mice). Both male and female mice were included in this study. Unexpectedly, we found that 4R NM human tau (hTau) exhibited abnormal dynamics in young mice that were lost with the P301L mutation, including elevated protein stability and hyperphosphorylation, which were associated with cognitive impairment in 5-month-old rT1 mice. Hyperphosphorylation of NM hTau was observed as early as 4 weeks of age, and transgene suppression for the first 4 or 12 weeks of life prevented abnormal molecular and cognitive phenotypes in rT1, demonstrating that NM hTau pathogenicity is specific to postnatal development. We also show that NM hTau exhibits stronger binding to microtubules than P301L hTau, and is associated with mitochondrial abnormalities. Overall, our genetically matched mice have revealed that 4R NM hTau overexpression is pathogenic in a manner distinct from classical aging-related tauopathy, underlining the importance of assaying the effects of transgenic disease-related proteins at appropriate stages in life.SIGNIFICANCE STATEMENTDue to differences in creation of transgenic lines, the pathological properties of the P301L mutation confers to the tau proteinin vivohave remained elusive, perhaps contributing to the lack of disease-modifying therapies for tauopathies. In an attempt to characterize P301L-specific effects on tau biology and cognition in novel genetically matched transgenic mouse models, we surprisingly found that nonmutant human tau has development-specific pathogenic properties of its own. Our findings indicate that overexpression of 4-repeat human tau during postnatal development is associated with excessive microtubule binding, which may disrupt important cellular processes, such as mitochondrial dynamics, leading to elevated stability and hyperphosphorylation of tau, and eventual cognitive impairments.