bluuebunny/crossref_metadata_2025_split huggingface dataset:
The unique role of seed banking and cryobiotechnologies in plant conservation
Authors: Walters Christina , Pence Valerie C. 
Published: August 2020

Societal Impact StatementEroding plant diversity has serious implications for the well‐being of humanity and our planet. Conserving plants ex situ requires technologies that are rapidly advancing and readily accessible. The alarming loss of plant habitats has spawned global investment in technologies that focus on either conventional freezer storage, which exploit seed adaptations to survive drying, or on cryogenic platforms, that ensure long‐term survival of germplasm that is not amenable to conventional methods. Increasing evidence that germplasm survives for decades provide proof of concept, but also warns of the limited utility of stored germplasm that is not returned to the Earth.SummaryA future sustainable world requires concerted efforts to conserve plant biodiversity. Using an integrated approach, botanic gardens, arboreta, universities, governmental agencies, and non‐governmental organizations are addressing that challenge. Here, we summarize some of the technological advances, in an ever‐growing toolbox, that increase the scope of taxa that are conserved ex situ as well as the lifespans of diverse plant tissues that can be used as germplasm. Seed banking continues to be a powerful and efficient tool. Seeds that tolerate extreme drying and low temperature will likely survive at least 100 years using conventional conditions of a common freezer. The extreme tolerance of seeds among diverse taxa has led to the global growth of seed banks to over 1,750 currently, and the conservation of over 50,000 species. Not all plants produce seeds or seeds that survive freezer conditions. Predictive models provide insight into the extent of taxa needing alternative strategies and an initial list of such species is available. These “exceptional” species require cryobiotechnologies (cryogenic storage in liquid nitrogen and in vitro technologies), which provide effective, long‐term ex situ conservation for a wide variety of tissues beyond seeds. The application of cryobiotechnologies increases the potential for conserving all plant biodiversity. Restoration of plant biodiversity into the future will require institutional collaborations among living collections, seed banks, and cryobanks to ensure technology transfer, information gathering and sharing, and capacity building in centers of biodiversity.
A new method for protein characterization and classification using geometrical features for3Dface analysis: An example of tubulin structures
Authors: Di Grazia Luca , Aminpour Maral , Vezzetti Enrico , Rezania Vahid , Marcolin Federica , Tuszynski Jack Adam 
Published: August 2020

AbstractThis article reports on the results of research aimed to translate biometric 3D face recognition concepts and algorithms into the field of protein biophysics in order to precisely and rapidly classify morphological features of protein surfaces. Both human faces and protein surfaces are free‐forms and some descriptors used in differential geometry can be used to describe them applying the principles of feature extraction developed for computer vision and pattern recognition. The first part of this study focused on building the protein dataset using a simulation tool and performing feature extraction using novel geometrical descriptors. The second part tested the method on two examples, first involved a classification of tubulin isotypes and the second compared tubulin with the FtsZ protein, which is its bacterial analog. An additional test involved several unrelated proteins. Different classification methodologies have been used: a classic approach with a support vector machine (SVM) classifier and an unsupervised learning with a k‐means approach. The best result was obtained with SVM and the radial basis function kernel. The results are significant and competitive with the state‐of‐the‐art protein classification methods. This leads to a new methodological direction in protein structure analysis.
Residential mobility of couples around family formation in the Netherlands: Stated and revealed preferences
Authors: Kooiman Niels 
Published: August 2020

AbstractDespite ongoing debates on urban resurgence and suggestions that cities have become more popular as a location to raise children, empirical evidence on the extent to which families with children tend to stay in cities is scarce. The aim of this paper is to fill this gap by analysing intentions to move and the actual mobility behaviour of young families in the Netherlands. First, and based on administrative data from Statistics Netherlands (2008–2016), it provides a detailed geographical analysis of couples' actual moving behaviour around the life event of first childbirth in a wide array of settlement types. Results demonstrate that the transition to parenthood still triggers couples to move down the urban hierarchy: urban couples, especially those in high‐density neighbourhoods, tend to leave the city, whereas couples in smaller municipalities outside the city tend to adjust their housing situation by local moves. However, lower density neighbourhoods in inner cities are relatively successful in retaining couples around family formation. Second, and based on the Netherlands' Housing Survey linked to administrative data, this paper examines geographical variations in families' stated intentions to move and analyses the likelihood that these moving intentions are realised. Results suggest that patterns of family migration cannot merely be interpreted as revealed preferences for suburban or rural environments. Whereas urban families are more likely than non‐urban families to have intentions to leave, they are also most likely to intend to move within their settlement. However, Amsterdam families in particular are unlikely to fulfil their intentions to make a local move, probably because of housing market constraints.
IgG4‐related lung disease progressing to respiratory failure
Authors: Kunimatsu Yusuke , Tani Nozomi , Sato Izumi , Ogura Yuri , Hirose Kazuki , Takeda Takayuki 
Published: August 2020

AbstractImmunoglobulin G4 (IgG4)‐related disease (IgG4‐RD) is a systemic immune‐mediated, fibroinflammatory disorder categorized as proliferative or fibrotic depending on the responsiveness of corticosteroid treatment. IgG4‐related lung disease (IgG4‐RLD) accounts for 13–14% of IgG4‐RD cases, but respiratory failure is quite rare. A 71‐year‐old man diagnosed with interstitial lung disease was referred to our department after a 10‐month observational period. He presented with respiratory failure at the first visit, with significant elevations in serum IgG4 levels and histopathological findings meeting the criteria of IgG4‐positive plasma cells and IgG4/IgG‐positive plasma cell ratio in transbronchial lung biopsy and inguinal lymph node biopsy, resulting in a diagnosis of IgG4‐RD. Systemic corticosteroid treatment promptly ameliorated the respiratory failure. 18F‐fluorodeoxyglucose (FDG) positron emission tomography/computed tomography showed significant FDG accumulation in the lung fields, indicating the proliferative and reversible status of IgG4‐RLD, which responded well to corticosteroid treatment. The patient recovered from respiratory failure even after a 10‐month observational period.
Optimal robust fault detection of discrete‐time LPV systems with measurement error‐affected scheduling variables combining ZKF and pQP
Authors: Tan Junbo , Olaru Sorin , Xu Feng , Wang Xueqian , Liang Bin 
Published: August 2020

SummaryOptimal robust state estimation (SE) and fault detection (FD) methods of discrete‐time linear parameter varying systems with measurement error‐affected scheduling variables are proposed under the boundedness assumption of system uncertainties. By using the weighted Frobenius norm of the generator matrix of SE zonotope to characterize the set size, the optimal observer gain can be computed by a zonotopic Kalman filter (ZKF) procedure for the purpose of observation. Meanwhile, by minimizing the influence of system uncertainties while maximizing that of faults on SE to enhance the sensitivity of FD, the optimal FD criterion is characterized based on an on‐line fractional programming problem, which can be equivalently transformed into a parametric quadratic programming (pQP) problem. The pQP problem can be efficiently solved by searching the root of its nonlinear characteristic equation using secant method. In general, as long as sensors with sufficiently high precision are equipped to measure the scheduling variables, the bounds of measurement errors of scheduling variables can be less conservative than those direct bounds of scheduling variables, which can reduce the conservatism of FD or SE in this way. At the end of this article, a case study based on a practical circuit model is used to illustrate the effectiveness of the proposed method.
Injectable BMP-2 gene-activated scaffold for the repair of cranial bone defect in mice
Authors: Sun Kai , Lin Hang , Tang Ying , Xiang Shiqi , Xue Jingwen , Yin Weifeng , Tan Jian , Peng Hao , Alexander Peter G. , Tuan Rocky S. , Wang Bing 
Published: August 2020

Abstract
Tissue engineering using adult human mesenchymal stem cells (MSCs) seeded within biomaterial scaffolds has shown the potential to enhance bone healing. Recently, we have developed an injectable, biodegradable methacrylated gelatin-based hydrogel, which was especially effective in producing scaffolds in situ and allowed the delivery of high viable stem cells and gene vehicles. The well-demonstrated benefits of recombinant adeno-associated viral (rAAV) vector, including long-term gene transfer efficiency and relative safety, combination of gene and cell therapies has been developed in both basic and translational research to support future bone tissue regeneration clinical trials. In this study, we have critically assessed the applicability of single-step visible light (VL) photocrosslinking fabrication of gelatin scaffold to deliver rAAV encoding human bone morphogenetic protein-2 (BMP-2) gene to address the need for sustained BMP-2 presence localized within scaffolds for the repair of cranial bone defect in mouse model. In this method, rAAV-BMP-2 and human bone marrow-derived MSCs (hBMSCs) were simultaneously included into gelatin scaffolds during scaffold formation by VL illumination. We demonstrated that the subsequent release of rAAV-BMP-2 constructs from the scaffold matrix, which resulted in efficient in situ expression of BMP-2 gene by hBMSCs seeded within the scaffolds, and thus induced their osteogenic differentiation without the supplement of exogenous BMP-2. The reparative capacity of this novel stem cell-seeded and gene-activated scaffolds was further confirmed in the cranial defect in the severe combined immunodeficiency mice, revealed by imaging, histology, and immunohistochemistry at 6 weeks after cranial defect treatment.

Significance statement This article describes a novel and advanced method of providing recombinant adeno-associated viral (rAAV)-human bone morphogenetic protein-2 (BMP-2) to human bone marrow-derived mesenchymal stem cells, a promising source of cells for regenerative medicine, encapsulated in the visible light photocrosslinking (VL-PXL) technology fabricated gelatin scaffold. In particular, live-dead staining and rAAV release kinetics assay were performed, to estimate the safety and efficiency of the scaffold-based delivery system. The in vivo study further showed the capacity of this new system in repairing the bone defect. This novel gene-engineered, cell-based, VL-PXL fabricated bone construct thus represents a robust treatment method for the mouse cranial defect.
Oxidative Amidation of Aldehydes with Amines Catalysed by Fe(II) – Hydride Complex and N‐ Heterocyclic Carbenes (NHC)
Authors: Singh Ashmita , Azad Chandra S. , Narula Anudeep K. 
Published: August 2020

AbstractOxidative amidation reaction of aldehydes with amines, by tert butyl hydrogen peroxide in toluene was achieved. The reaction utilised the in situ generated catalyst produced by Fe(II) – hydride complex [FeH2(PPh3)4], and N‐heterocyclic carbene (NHC) ligand. The catalytic system was found efficient for the synthesis of wide variety of mono and disubstiuted amides.
In Silico Identification of Potential Inhibitors of ADP‐Ribose Phosphatase of SARS‐CoV‐2 nsP3 by Combining E‐Pharmacophore‐ and Receptor‐Based Virtual Screening of Database
Authors: Debnath Pradip , Debnath Bimal , Bhaumik Samhita , Debnath Sudhan 
Published: August 2020

AbstractThe recently emerged 2019 Novel Coronavirus (SARS‐CoV‐2) and associated COVID‐19 disease cause serious or even fatal respiratory tract infection. Observing the spread, illness and death caused by COVID‐19, the World Health Organization (WHO) declared COVID‐19 a pandemic. To date, there is no approved therapeutics or effective treatment available to combat the outbreak. This urgent situation is pressing the world to respond with development of novel vaccine or a small molecule therapeutics for SARS‐CoV‐2. In line with these efforts, the structure of several proteins of SARS‐CoV‐2 has been rapidly resolved and made publicly available to facilitate global efforts to develop novel drug candidates. In this paper, we aim to find out the small molecule inhibitors for ADP‐ribose phosphatase of SARS‐CoV‐2. In order to identify potential inhibitors, we applied sequential E‐pharmacophore and structure‐based virtual screening (VS) of MolPort database containing 113687 number of commercially available natural compounds using Glide module. Six potential inhibitors having admirable XP glide score range from −11.009 to −14.684 kcal/mol and good binding affinity towards active sites were identified. All the molecules are commercially available for further characterization and development by scientific community. The in vitro activity of selected inhibitors can be done easily which will provide useful information for clinical treatment of novel coronavirus pneumonia.
Mechanistic Insight into Selective Sensing of Hazardous Hg2+ and Explosive Picric Acid by Using a Pyrene‐Azine‐Hydroxyquinoline Framework in Differential Media
Authors: Mondal Tapashree , Mondal Indranil , Biswas Sourav , Mane Manoj V. , Panja Sujit S. 
Published: August 2020

AbstractA pyrene‐hydroxyquinoline tethered ingeniously designed and developed azine based Schiff base luminophoric system, 2‐((Z)‐((E)‐(pyren‐1‐ylmethylene)hydrazono)methyl)quinolin‐8‐ol (PHQ) has been explored as a selective ‘TURN ON’ chromofluorogenic sensor for hazardous Hg2+ ions in ethanol/H2O (9 : 1,v/v) medium with detection limit of 0.22 μM and binding constant of 1.09 x 103 M−1. The detail scrutiny of mechanism of interaction between PHQ and Hg2+ is rationalized through various techniques like 1H NMR titration, FT‐IR, mass spectral analysis, theoretical computations, Job's plot, dynamic light scattering (DLS), transmission electron microscopy (TEM), time resolved excited state decay dynamics and fluorescence reversibility studies, which concretely declare a synergistic effect of supramolecular aggregation induced enhanced emission and deactivation of photo‐induced electron transfer (PET) processes. On the other hand, a ‘TURN OFF’ luminescence feature of highly fluorescent PHQ hydrosol is utilized for the selective screening of powerful explosive picric acid (PA) in aqueous medium and mechanism is deciphered by means of steady state, time‐resolved emission as well as computational studies which altogether reflect a combined effect of PET and ground state complexation between probe and PA. The commendable detection limit of 55 nM and Stern‐Volmer quenching constant of 1.48 x 104 M−1 with high quenching efficiency of 84% increases the pertinency of the nano‐probe.
Highly Selective and Sensitive (PPB Level) Quinolin‐Based Colorimetric Chemosensor for Cu(II)
Authors: Mohan Vaisakh , Das Nilimesh , Jain Vipin K. , Khan Tanmoy , Pandey Sarvesh K. , Faizi Md. Serajul H. , Daniel Joseph , Sen Pratik 
Published: August 2020

Abstract4‐(quinolin‐2‐ylmethylene)aminophenol (QMAP) is synthesised in a one‐step synthetic process and was checked for its sensing ability towards metal ions. The molecule is found to be effective for the instantaneous selective and sensitive Cu(II) ion detection in PPB level in methanolic solution with a distinct development of orangish yellow colour from a colourless solution, which can be detected visually. The sensing ability of the QMAP towards Cu(II) is found independent on the type of counter anions and also in the presence of other metal ions. QMAP does not show any colour change in the presence of other metal salts. A 2 : 1 binding stoichiometry between QMAP and Cu(II) with a large binding constant of 3.2 x 1010 M−2 has been measured. Quantum chemical calculations have been used to understand the nature of the complex and the mechanism of the sensing.
The Ultrasmall Palladium Nanoparticles Catalyzed Telomerization of CO2 with 1,3‐Butadiene at Room Temperature: Selective Synthesis of δ‐Lactone
Authors: Wang Jixiao , Feng Xiujuan , Song Jiliang , Gao Zhanming , Wang Wan‐Hui , Bao Ming 
Published: August 2020

AbstractAn efficient method for the selective synthesis of δ‐lactone from CO2 and 1,3‐butadiene was developed by using ultrasmall palladium nanoparticles as a catalyst for the first time. Palladium nanoparticles (1.90±0.39 nm) were generated in situ in the presence of Pd(acac)2, tetrabutylammonium acetate (TBAAc), and PPh3 as the precatalyst, stabilizer, and reductant, respectively. Maximum yield (86 % with 96 % selectivity) with satisfactory conversion (89 %) and turnover number (1271) was achieved under mild conditions.
Highly Enhanced OER Activity of Amorphous Co3O4 via Fabricating Hybrid Amorphous‐Crystalline Gold Nanostructures
Authors: Muthukumar Pandi , Ranganathan Prabha , Pannipara Mehboobali , Al‐Sehemi Abdullah G. , Anthony Savarimuthu Philip 
Published: August 2020

AbstractHybrid nanostructures of amorphous cobalt oxide (Co3O4) and crystalline gold nanoparticles (AuNPs) showed strongly enhanced OER activity compared to pure amorphous Co3O4. Particularly, gold nanorods (Au NRs) integrated amorphous Co3O4 nanostructures (aCo3O4/Au NRs) required significantly low overpotential (225 mV) to produce geometric current density and needed 336 and 398 mV for achieving current density of 100 and 400 mA/cm2, respectively. Further, aCo3O4/Au NRs displayed good stability over 40 h at 336 mV.
Design and Synthesis of Coumarin‐Based Pyrazolopyridines as Biocompatible Fluorescence Dyes for Live‐Cell Imaging
Authors: Alizadeh Abdolali , Farajpour Behnaz , Mohammadi Sanam Sadeghi , Sedghi Mosslim , Naderi‐Manesh Hossein , Janiak Christoph , Knedel Tim‐Oliver 
Published: August 2020

AbstractAn unprecedented strategy for the synthesis of highly fluorescent chromeno[4,3‐b]pyrazolo[3,4‐d]pyridin‐4(3H)‐ones via a 1,3‐dipolar cycloaddition reaction/ring closure in the presence of NH4OAc/air oxidation in a one‐pot procedure is described. The molecular structures were characterized by IR spectrophotometry, mass spectrometry, NMR spectroscopy, and single‐crystal X‐ray analysis. Furthermore, the photophysical properties of synthesized compounds were studied by absorption and fluorescence spectroscopy. All of the compounds exhibited almost similar spectra due to their resembling molecular structures. Therefore, one of the synthesized coumarin‐based pyrazolopyridines was selected to evaluate its application as a live‐cell imaging agent by an inverted phase‐contrast microscope. The result reveals that synthesized chromeno[4,3‐b]pyrazolo[3,4‐d]pyridin‐4(3H)‐one has acceptable photostability, low cytotoxicity, satisfactory fluorescence efficiency (Φf: 0.53), and excellent capability to penetrate into the intracellular space. Accordingly, it can be rendered as a promising dye in fluorescence bioimaging. Chemoselectivity, simple and available starting materials, operational simplicity, and good yields are other outstanding advantages of this reaction.
Electrochemical Sensing of Paracetamol Using Electropolymerised and Sodium Lauryl Sulfate Modified Carbon Nanotube Paste Electrode
Authors: Manjunatha Charithra Madikeri , Manjunatha Jamballi G. 
Published: August 2020

AbstractThe sensing tool based on the electropolymerisation of alizarin carmine (AC) and immobilization of sodium lauryl sulfate (SLS) on the surface of bare carbon nanotube paste electrode (BCNTPE) has been developed. The developed ultrasensitive electrochemical device employed for the voltammetric analysis of paracetamol (PR). Poly (AC) SLS modified CNTPE (PACSLSMCNTPE) facilitates the enhancement in voltammetric signal for PR in 0.2 M supporting electrolyte (pH 7.0). The surface morphology features of the proposed electrodes were gained from the field emission scanning electron microscopy (FE‐SEM). The calibration curve for PR concentration level from 4.0‐100 μM was plotted and 0.06 μM limit of detection (LD), 0.21 μM limit of quantification (LQ) was attained. The designed sensor unveiled with sufficient repeatability, satisfactory reproducibility, and acceptable stability. No major disturbance in the voltammetric signal for PR in the existence of dopamine (DA) and estriol (ES) discloses the selectivity of the proposed sensor. The projected sensor was magnificently applied for the electroanalysis of PR in the tablet formulations.
In Situ Formation of Ag Nanoparticles Capped by Photoactivable Ligands for Optical Sensing of Hypochlorite
Authors: Tang Hong , Wang Xu , Huang Xiaohuan 
Published: August 2020

AbstractPhotomediated ligands afford powerful tools to improve the spatial and temporal resolution in the clinical diagnosis and environmental monitoring. In this work, a photoactivable ligand was designed by attachment of a pyrene unit to the strained cyclic disulfide of lipoic acid. Co‐incubation of the ligand with AgNO3 under sunlight induce the in situ formation of Ag nanoparticles with a remarkable fluorescence enhancement. By integration of fluorescent and plasmonic modalities into one structure, the obtained Ag nanoparticles exhibit a dual‐mode sensing to hypochlorite with fast response and high selectivity. This method could become an easy‐to‐obtain, ready‐to‐use, and eco‐friendly way to detect hypochlorite.
The Crucial Role of Environmental Coronas in Determining the Biological Effects of Engineered Nanomaterials
Authors: Xu Lining , Xu Ming , Wang Ruixia , Yin Yongguang , Lynch Iseult , Liu Sijin 
Published: August 2020

AbstractIn aquatic environments, a large number of ecological macromolecules (e.g., natural organic matter (NOM), extracellular polymeric substances (EPS), and proteins) can adsorb onto the surface of engineered nanomaterials (ENMs) to form a unique environmental corona. The presence of environmental corona as an eco–nano interface can significantly alter the bioavailability, biocompatibility, and toxicity of pristine ENMs to aquatic organisms. However, as an emerging field, research on the impact of the environmental corona on the fate and behavior of ENMs in aquatic environments is still in its infancy. To promote a deeper understanding of its importance in driving or moderating ENM toxicity, this study systemically recapitulates the literature of representative types of macromolecules that are adsorbed onto ENMs; these constitute the environmental corona, including NOM, EPS, proteins, and surfactants. Next, the ecotoxicological effects of environmental corona‐coated ENMs on representative aquatic organisms at different trophic levels are discussed in comparison to pristine ENMs, based on the reported studies. According to this analysis, molecular mechanisms triggered by pristine and environmental corona‐coated ENMs are compared, including membrane adhesion, membrane damage, cellular internalization, oxidative stress, immunotoxicity, genotoxicity, and reproductive toxicity. Finally, current knowledge gaps and challenges in this field are discussed from the ecotoxicology perspective.