bluuebunny/crossref_metadata_2025_split huggingface dataset:
Omics multi-layers networks and identification of genes involved in fat storage and metabolism in poultry using interactomics approach
Authors: bahrami abolfazl , Ghafouri Farzad , Sadeghi Mostafa , Miraei-Ashtiani Seyed Reza 
Published: June 2020

Abstract
Background

Fatty acid metabolism in animals has a major impact on production and disease resistance traits. According to the high rate of interactions between lipid metabolism and its regulating properties, a holistic approach is necessary.
Methods

To study multi-omics layers of adipose tissue and identification of genes involved in fat metabolism, storage and endocrine signaling pathways in two groups of broiler chickens with high and low abdominal fat, high-throughput screening (HTS) techniques were used. The Gene-miRNA interacting bipartite and metabolic-signaling networks were reconstructed using their interactions.
Results

In the analysis of microarray and RNA-Seq data, 1835 genes were detected by comparing the identified genes with significant expression differences. Then, by comparing, 34 genes and 19 miRNAs were detected as common and main nodes. The literature mining approach was used and 7 genes were identified and added to the common gene set. Module finding revealed three important and functional modules. The detected modules 1, 2, and 3 were involved in the PPAR signaling pathway, biosynthesis of unsaturated fatty acids, and Alzheimer's disease metabolic pathway, adipocytokine, insulin, PI3K-Akt, mTOR and AMPK signaling pathway.
Conclusions

This approach revealed a new insight for a better understanding of the biological processes associated with adipose tissue.
The acute effect of cold pack applied for different periods on the biomechanical properties of the rectus femoris muscle of healthy individuals: a randomized experimental study
Authors: ALACA Nuray , Kablan Nilüfer 
Published: June 2020

Abstract
Background

Cold packs are silica gel packs that are the most commonly used modalities in spots clinics. In the present study, we investigated the acute effect of different periods of cold pack application, on the biomechanical properties of the rectus femoris muscle of healthy individuals.
Methods

This study is a randomized experimental study. Sixty individuals (18–23 age) were randomly divided into four groups. The cold packs were applied for 10 minutes (Group 1), 12 minutes (Group 2), 15 minutes (Group 3), and 20 minutes (Group 4). The temperature of the skin (thermal camera) and the biomechanical properties evaluations of the muscle (Myoton Pro) were measured (before and immediately after, 5, 10, 15, 20 and 30 minutes after the application).
Results

After the application, while Group 1 and 2 showed an increase in muscle tone and stiffness and a decrease in elasticity (p < 0.05), they began to approach their pre-application state by the fifth and fifteenth minutes, respectively (p > 0.05). In Group 3 and 4, the muscle stiffness was increased at all time points (p < 0.05).
Conclusions

The results of this study show that the rectus femoris muscle of healthy people becomes stiffer and less elastic as a result of cooling with a cold pack applied for different time periods. The duration that affects the biomechanical properties of the muscle least and provides optimum cooling is the 10-minute cold pack application.
Trial registration:

Ethical approval The Research Ethics Committee of Acıbadem University and Acıbadem Healthcare Group has approved the study (reference no. ATADEK-2019/17/19) and retrospectively registered clinical trial number (NCT04277481, February 20, 2020). All procedures performed in studies involving human participants were in accordance with the 1964 Helsinki Declaration. Verbal and written consent was obtained from all participants.
Early low-dose ghrelin intervention via mini-osmotic pumps could protect against the progressive dopaminergic neuron loss in Parkinson’s disease mice
Authors: Jiao Lingling , Du Xixun , Jia Fengju , Zhang Pei , Li Yong , Zhu Dexiao , Tang Tinging , Jiao Qian , Jiang Hong 
Published: June 2020

Abstract
BackgroundGhrelin has been identified as a multifunctional peptide that has a potential applications for the treatment Parkinson’s disease (PD). Method: ELISA was used for detecting plasma total and active ghrelin levels, dopamine (DA) content was measured by HPLC-ECD, immunofluorescence staining and Western blot were used to detect protein expressions, and cytokine was tested by Bio-PlexPro™ assay.ResultsHere, we reported a subcutaneous administration of low-dose ghrelin via mini-osmotic pumps to PD mice. The decreased levels of total and active ghrelin in plasma were rescued by ghrelin administration. Interestingly, ghrelin had no effect on weight gain in wild-type mice but improved weight loss in PD mice. We observed the attenuation of dopaminergic (DAergic) neuron loss in substantia nigra (SN) and low level of dopamine content in the striatum in PD mice with ghrelin treatment. Ghrelin administration could improve the environment of DAergic neuron by inhibiting microglia proliferation and pro-inflammatory cytokine expression, and could enhance cell survival by upregulating Bcl-2/Bax ratio and superoxide dismutase1 (SOD1) protein level in SN in PD mice.ConclusionsOur results suggested that subcutaneous administration of low-dose ghrelin could prevent the onset or the progression of PD, and also provided a possible method for ghrelin application to cure PD.
Clinical characteristics and health care cost among patients successfully treated for COVID-19 in Henan, China: A Descriptive Study
Authors: Miao Yudong , Bhuyan Soumitra S , Liu Mengzhu , Gu Jianqin , Hussain Sabiha , Wu Jian , Wang Liuyi , Liu Min , Cheng Ruirui , Basu Urmi , Liu Feng , Zhang Huayu , Chang Zhanjun 
Published: June 2020

Abstract
Objective: To clarify the clinical and medical expense characteristics of COVID-19. Methods: In this retrospective, single-center study, 55 cured cases with confirmed COVID-19 were analyzed for demographic, epidemiological, clinical, and radiological features and medical expense data.Results: The average age of the 54 successfully treated patients with COVID-19 was 53.2 years old (SD 19.0), including 27 men and 27 women. Off this, 31 (57.4%) patients had chronic diseases. Patients commonly had clinical manifestations of fever (45 [83.3%] patients), cough (29[54.7%] patients), expectoration (28 [51.9%] patients), fatigue (24[44.4%] patients) and diarrhea (8[14.8%] patients) on admission. There was a 10-day interval from the onset of signs and symptoms to hospital admission. About 80% of them got recovery after a two-week treatment. The mean interval from the onset of signs and symptoms to hospital discharge was 20.5 (IQR 16-29) days. The median total medical expense of the treated patient, in general, was 2579.6 (IQR 1366.1-4837.6) U.S. dollars. Still, the median medical expense was 8904.1 (IQR 6660.1- 27143.8) U.S. dollars in patients with more than five comorbid illnesses during the treatment.Conclusion: There is a 3-week interval from the onset of signs and symptoms to cure, and most hospitalized patients get recovery within two weeks. The total medical expense of cases with more than five comorbid conditions during the treatment is higher. Quite a few COVID-19 cases with other serious diseases are likely to account for most of the total medical expenses.
Preoperative Elevated International Normalized Ratio Cannot Increase Transfusion or Complication in Primary Total Hip Arthroplasty: A Retrospective Study of 552 Cases
Authors: Peng Linbo , Zeng Junfeng , Zeng Yi , Wu Yuangang , Yang Jing , Shen Bin 
Published: June 2020

Abstract
Background: Verify if the elevated preoperative International Normalized Ratio (INR) can increase transfusion and complication rate in primary total hip arthroplasty.Methods: We retrospectively reviewed the database of adults who underwent primary total hip arthroplasty between 2014 to 2018 by the same surgeon. 552 cases were assigned into 3 groups by preoperative INR class: INR≤0.9, 0.9＜INR＜1.0, and INR≥1.0 eventually. We regarded the transfusion rate as the primary outcome. We also included perioperative blood loss, maximum Hb-drop, postoperative anemia needs medicine, length of stay (LOS), re-operation, the complication rate in 90 days and mortality as the secondary outcomes. Univariable analyses were utilized to compare baselines and outcomes between groups. Binary Logistic Regression was used to adjust differences of baselines among groups.Results: All the cases had an INR＜1.5. Among all the cases, 93(16.8%) had INR≤0.9, 268 (48.6%) had 0.9＜INR＜1.0, and 191 (34.6%) had INR≥1.0, respectively. In the univariable analyses, with the INR elevated, The transfusion rates increased from 1.08% for INR≤0.9, 1.12% for 0.9＜INR＜1.0 to 5.76% for INR≥1.0 (p＜0.05). The overall complication rate increased from 10.8% for INR≤0.9, 16.4% for 0.9＜INR＜1.0 to 22.5% for INR≥1.0 (p＜0.05). When controlling for the demographics and comorbidities characteristics, there was no statistically significant difference when evaluating the odds of transfusion nor overall complication rate between the groups (p＞0.05).Conclusions: The transfusion and complication rate cannot increase along with the INR elevated in primary THA. With the improvement of arthroplasty protocol and use of tranexamic acid, the INR＜1.5 was still a conventional safe threshold.
The effect and long-term outcome of Partial Anterior Myometrial Resection and Reconstruction under tourniquet and/or prophylactic abdominal aorta balloon occlusion on the patients with placenta percreta
Authors: Zhao Huidan , Chen Chen , Tao Ya , Liu Zhuan , Liu Cai , Zhao Xianlan 
Published: June 2020

Abstract
Background

Patients with placenta accreta spectrum (PAS) disorders often experience overwhelming hemorrhage during cesarean. Placenta percreta is the most serious subtype. In this paper, we propose a new procedure in order to control intraoperative bleeding. We aimed to evaluate the effect and long-term outcome of Partial Anterior Myometrial Resection and Reconstruction under tourniquet and/or prophylactic abdominal aorta balloon occlusion on patients with placenta percreta.
Methods

In a retrospective study, data from patients with placenta percreta who delivered by cesarean section between January 1, 2017 and December 31, 2019 were analyzed. Short-term and long-term outcomes were followed up by outpatient clinic and by phone. The quantity of estimated blood loss (EBL), operation time, urine tube time, hospital stay and short-term and long-term complications including fever, thrombosis, hematoma, breast feeding and menstruation problems, lower limb complaints, intrauterine adhesion and so on, were analyzed.
Results

For all cases, the mean EBL in the surgery was 1399 ± 948 mL, the mean operation time was 107 ± 35minutes, and the mean perioperative hospital stay was 9.6 ± 5.0 days. All the patients had a preserved uterus. Menstrual quantity decreased in 12 patients. Menstrual period prolonged in 11 cases. Uncomfortable abdomen symptoms happened in 9 cases after the surgery. Four cases got thrombosis and 3 got hematoma. Lower extremity discomfort was found in 6 patients.
Conclusion

Combined with prophylactic abdominal aorta balloon occlusion and/or tourniquet, Partial Anterior Myometrial Resection and Reconstruction is highly effective in reducing the intraoperative blood loss and hysterectomy in placenta percreta. But we should pay great attention to short-term and long-term complications, especially to the complications associated with aorta balloon occlusion.
Screening COVID-19 with Chest CT in Preference to RT-PCR: Based on Mortality Analysis
Authors: Xie Yuanlong , Wu Minhao , Zhang Chong , Zhu Yufan , Li Kun , Wu Meng , Deng Zhouming , Zhu Xiaobin , Cai Lin 
Published: June 2020

Abstract
Corona Virus Disease 2019 (COVID-19) became the outbreak of infectious diseases emergency worldwide. It remains unknown whether the RT-PCR test results was associated with the prognosis of COVID-19 patients or not. In this study, a total number of 495 patients with typical chest CT feature and symptom consistent with COVID-19 were retrospectively included from Jan 23, 2020 to Feb 26, 2020. 186 (37.58%), 32 (6.46%) and 277 (55.96%) COVID-19 patients had initial positive, suspected and negative RT-PCR results, respectively. The mean age was 58.55 years and 254(51.3%) were older than 60 years. 60.00% (297/495), 22.02% (109/495) and 17.98% (89/495) of COVID-19 patients were common, severe and critically type, respectively. There were no significant differences of age, gender, time from onset to hospitalization and severity classification in the patients with initial positive and negative RT-PCR result. The mortality rate of patients with positive and negative were 7.14% and 7.94%. Patients with initial negative or initial positive RT-PCR results had no significant difference of mortality rate (c2=4.079, p=0.130). The number of patients with lymphocyte ratios under the normal level was significantly larger in patients with initial negative RT-PCR results (59/92) compared with the patients with initial positive result (86/167), p=0.033. COVID-19 patients with positive or negative RT-PCR results had no significant difference in severity and mortality. Chest CT may be a more effective tool to screen COVID-19 in preference to RT-PCR.
Comparative transcription analysis of hard and tender fruit spines of cucumber to identify genes involved in morphological development of fruit spines
Authors: Lv Duo , Yu Yao , Xiong Liang-Rong , Wang Gang , Pang Jin-An , Sun Jing-Xian , Zhang Ke-Yan , He Huan-Le , Cai Run , Pan Junsong 
Published: June 2020

Abstract
Background
The trichomes of cucumber on the fruit are also called spines, it not only has important commercial value but also is a classical tissue to study cell division and differentiation mode of multicellular trichomes. Although there have been many researches about the development of unicellular trichomes in model plants, the molecular mechanism of multicellular trichomes formation remains elusive. In this study, we took a pair of cucumber materials defined as hard (Ts, wild-type) and tender spines (ts, mutant) in previous study, the whole developmental process of fruit spines was continuously observed by microscope and SEM, in an attempt to define the development stage of fruit spines, transcriptome profiles at different stages was made to explore the molecular mechanism in the process of spines development.
Results
With significant phenotypic differences, the developmental process of fruit spines was clearly defined as four stages. Comparion of transcriptome profilings showed a total of 2,788 differential expression genes (DEGs) between the wild-type and mutant at different developmental stages of fruit spines, these genes exhibited different expression patterns at different spines developmental stage. Some DEGs related to cell cycle and meristem had also been identified in transcriptome data. By correlating the expression patterns of several transcription factors that have been reported to be involved in the development of spines, we identified some genes that may be involved in the formation of cucumber fruit spines. The cluster analysis of transcription factors revealed that there were 26 transcription factor families that may be involved in the development of fruit spines, among them, the ERF-ERF, bHLH and WAKY family transcription factor gene families were the top three gene families.
Conclusions
We defined the four stages of fruit spines development and identified a number of genes that may be involved in the development of multicellular trichomes based on comparative transcription. The results provided a step stone for further analysis of the molecular mechanism in the developmental process of multicellular trichomes.
Preoperative Risk Grade Predicts the Long-Term Prognosis of Intrahepatic Cholangiocarcinoma: A Retrospective Cohort Analysis
Authors: Zhao Jianping , Chen Yao , Wang Jingjing , Wang Jian , Wang Ying , Chai Songshan , Zhang Yuxin , Chen Xiaoping , zhang wanguang 
Published: June 2020

Abstract
Background: Cumulating evidence indicates that the systemic inflammatory response (SIR) plays a crucial role in the prognosis of various cancers. We aimed to generate a preoperative risk grade (PRG) by integrating SIR markers to preoperatively predict the long-term prognosis of intrahepatic cholangiocarcinoma (ICC).Methods: 468 consecutive ICC patients who underwent hepatectomy between 2010 to 2017 were enrolled. The PRG and a nomogram were generated and their predictive accuracy was evaluated. Results: The PRG consisted of two non-tumor-specific SIR markers platelet-to-lymphocyte ratio (PLR) and albumin (ALB), which were both the independent predictors of overall survival (OS). Multivariate analysis showed that the PRG was significantly associated with OS (PRG =1: Hazard ratio (HR) = 3.800, p < 0.001; PRG = 2: HR = 7.585, p < 0.001). The PRG (concordance index (C-index) 0.685; 95% CI: 0.655 to 0.716) had a better predictive ability than the American Joint Committee on Cancer (AJCC) 8th edition (C-index 0.645; 95% CI: 0.612 to 0.679), the Liver Cancer Study Group of Japan (LCSGJ) (C-index 0.644; 95% CI: 0.611 to 0.678) and the Okabayashi (C-index 0.633; 95% CI: 0.600 to 0.666) (p < 0.05). Besides, the C-index of the nomogram only consisting of the tumor-specific factors (serum carcinoembryonic antigen, carbohydrate antigen 19-9, tumor number) could be improved to 0.737 (95% CI: 0.062-0.768) from 0.625 (95% CI: 0.585-0.665) when the PRG was incorporated (p < 0.001).Conclusions: The PRG integrating two non-tumor-specific SIR markers PLR and ALB was a novel method to preoperative predicting the prognosis of ICC.
Efficacy of percutaneous short segment fixation on the treatment of Magerl A3 thoracolumbar fractures: a retrospective study
Authors: lin hongheng , Ou Xiaoting , Li Wenchao , Chen Minyuan , Liu Caijun , Mo Ling , Yuan Chao , Zhu Hui , Liang De 
Published: June 2020

Abstract
BackgroundThis is a retrospective study to evaluate the efficacy of percutaneous short segment fixation (PSSF) with or without intermediate screws in Magerl A3 thoracolumbar (TL) fractures accompanied with low bone mineral density (BMD).Methods From January 2017 to December 2018, 30 patients diagnosed with Magerl A3 TL fractures having a BMD between 0.5/cm2 and 1g/cm2 in the lumbar vertebrae with intact neurological functions and a history of major trauma who underwent PSSF with or without intermediate screws were enrolled in this study. The patients were divided into two groups of those with intermediate screws (group F) or without (group S). The operation time and intra-operation blood loss were recorded. Oswestry disability index (ODI) questionnaire and visual analogue scale (VAS) were employed as clinical assessments and quantified. Radiographic follow-up assessed according to the Cobb angle (CA), Vertebral wedge angle (VWA), and anterior vertebral body height (AVBH).ResultsLoose screws were observed in 4 of the 30 enrolled patients. Significant differences were observed in operation time and intra-operative blood loss between the two groups. The enrolled patients’ clinical results (VAS and ODI scores) were good, however, no significant differences during all follow-up periods were present between the two groups. Radiographic evaluation (CA, VWA and AVBH) improved immediately after surgery, but significant correction loss was observed in the follow-up periods, especially in the first month. Radiographic evaluation demonstrated no significant differences between the two groups.Conclusion Although the patients’ clinical results were satisfactory, PSSF could not resist the correction loss in Magerl A3 TL fractures accompanied with BMD below 1g/cm2. Accordingly, the use of intermediate pedicle screws may be unnecessary due to their limited ability in resisting correction loss and increasing operation time as well as intra-operation blood loss.
Characterization of isolated thermophilic campylobacters and associated risk factors in poultry farms of Uttarakhand, India
Authors: Garhia Geetika , Mani Maansi , Kumar Deepak , Upadhyay Ajay Kumar 
Published: June 2020

Abstract
Background

Campylobacters are the common commensals of poultry responsible for several cases of gastroenteritis in humans. The illness, if severe can result into complications causing a nervous disorder named Guillian Barre syndrome. Owing to its serious health implications, the study aimed to screen eight organized poultry farms and their environment (water, litter, manure, and feed) of Uttarakhand state, India for the presence of thermophilic Campylobacter species and their virulence and antibiotic resistance profile. It also undertook identification of risk factors associated with the occurrence of campylobacters in each farm using a questionnaire survey comprising eleven potential risk factors (other animals on farm, reuse of litters, use of foot bath, in house or branded feed, chlorination of water, distance of manure heap, housing system, flock size, floor type, shoe use by farm personnels, moist or dry litter and number of broiler floor).
Results

Of eight, six farms showed varying occurrence of C.jejuni and C.coli with an overall prevalence of 12.29%. Not a single isolate of C.lari and C.upsaliensis was recorded. Poultry faecal, water and litter samples observed 18.2%, 6% and 1.9% presence, respectively. Feed and manure samples did not appear positive. In 48 revived Campylobacter isolates, 100% presence of cadF and flaA virulence genes were detected followed by cdtB (97.9%), cgtB (22.9%) and ciaB (12.5%), respectively. Ten isolates 23.80%) were multidrug resistant (MDR) exhibiting resistance to at least 3 or more antimicrobial classes. The most common MDR patterns were AMP CX CIP TE (n = 2) and AMP CX CIP (n = 2). Feeding of branded feed was found to have significant association with Campylobacter presence in the examined broiler flocks (p-value 0.0047).
Conclusions

The study highlights the occurrence of food pathogens, Campylobacter jejuni and C.coli in the poultry farms and their environment of the state. The organisms possessed significant virulence genes capable of developing critical human illness. Overall, the presence of MDR thermophilic campylobacters appears to be a severe public threat.
Effect of discharge conference on rehospitalization among older patients: An analysis of administrative claims data in Japan
Authors: Jeon Boyoung , Tamiya Nanako , Jin Xueying , Yoshie Satoru , Iijima Katsuya , Ishizaki Tatsuro 
Published: June 2020

Abstract
Background

The policy of discharge conference has function of guiding patients to stay at community. This study aims to investigate the effect of a discharge conference on a probability of hospital readmission and readmission costs among older patients in Japan.
Methods

We included 8,096 individuals admitted to acute care hospitals, using health and long-term care insurance claims data on older patients (age ≥ 75 years) in a suburban city in Japan, from April 2012 to September 2013. To balance the two groups according to whether a patient received a service of discharge conference or not, we used propensity score matching method. We identified readmission within 360 days from discharges and estimated the impact of a discharge conference on the probability of readmission and readmission costs using multiple logistic and linear regression model.
Results

Among patients who discharged from an acute care hospital, 367 (4.5%) received a discharge conference. Using the matching method, 304 participants in a control group was matched to 304 participants in a discharge conference group. Readmission rate was 21.1% in patients with a discharge conference and 23.0% in those without a discharge conference. Although there was no significant effect of discharge conference on probability of readmission, but it showed significant effect on lower cost per day.
Conclusions

These results imply a discharge conference has effect on mitigating cost per day of readmission after adjusting for confounding. This study suggests that there are potential possibilities in the policy of discharge conference on reducing the readmission costs per day amongst older patients.