bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel concepts in cervical cancer screening: a comparison of VIA, HPV DNA test and p16INK4a/Ki-67 dual stain cytology in Western Kenya
Authors: Orang’o Elkanah Omenge , Were Edwin , Rode Oliver , Muthoka Kapten , Byczkowski Michael , Sartor Heike , Vanden Broeck Davy , Schmidt Dietmar , Reuschenbach Miriam , von Knebel Doeberitz Magnus , Bussmann Hermann 
Published: October 2020

Abstract
Background
Screening of unvaccinated women remains essential to mitigate the high morbidity/mortality of cervical cancer. Here, we compared visual inspection with acetic acid (VIA), recommended by WHO as the most cost-effective screening approach in LMICs, with HPV-based screening, and usage of p16INK4a/Ki-67 dual stain cytology.

Methods
We prospectively enrolled women participating in a VIA-based cervical cancer screening program in two peri-urban health centers of Kenya. Consenting women had a VIA examination preceded by collection of a liquid-based cytology sample from the cervix stored in PreservCyt medium (Hologic®). Analysis of all samples included a hrHPV DNA test and evaluation of a p16INK4a /Ki-67 (CINtecPLUS®) dual stained slide that was prepared using the ThinPrep® 2000 Processor and evaluated by a pathologist trained in the methodology.

Results
In 701 of a total of 800 women aged 18–64 years, all three investigations were performed and data could be analyzed. The HPV, VIA and dual stain cytology positivity were 33%, 7%, and 2% respectively. The HPV positivity rate of VIA positive cases was 32%. The five most common HPV types were HPV16, 52, 68, 58 and 35. The OR among HIV infected women of an HPV infection, VIA positivity and positive dual stain cytology were 2.6 (95%CI 1.5–4.3), 1.9 (95%CI 0.89–4.4) and 3.4 (95%CI 1.07–10.9) respectively. The sensitivity of VIA to detect a p16INK4a/Ki-67 positive transforming infection was 13% (95%CI 2–38).

Conclusions
Primary HPV testing appears feasible and should be considered as a primary screening test also in LMICs. The poor sensitivity of VIA renders it unsuitable as a triage test for HPV positive women. The utility of p16INK4a/Ki-67 dual stain cytology as a triage test for HPV positive women in LMICs should be further studied.
Effect of vitamin A on intestinal mucosal injury in pediatric patients receiving hematopoietic stem cell transplantation and chemotherapy: a quasai-randomized trial
Authors: Pattanakitsakul Ploy , Chongviriyaphan Nalinee , Pakakasama Samart , Apiwattanakul Nopporn 
Published: October 2020

Abstract
Objective
Vitamin A is involved in maintenance of gut mucosal integrity and normal immune function. However, it is unclear whether these functions of vitamin A have any beneficial effects in patients undergoing hematopoietic stem cell transplantation (HSCT). In this study, we aimed to examine the potential protective effect of vitamin A supplementation on gastrointestinal (GI) mucosal integrity in HSCT recipients using plasma citrulline as a surrogate marker of intestinal integrity.

Results
We performed a quasi-randomized trial in 30 pediatric patients undergoing HSCT. Half (n = 15) of the patients received a single high dose of vitamin A (200,000 IU) before the conditioning regimen was given, and half (n = 15) did not. Clinical data of patients who developed post-transplant complications were recorded for 60 days after HSCT. There were no significant differences in mean plasma citrulline levels on day 7 after HSCT between the treatment and control groups (5.8 vs. 5.9 µmol/L, respectively). The incidence of mucositis and other complications were not different between the two groups within 60 days of HSCT. Vitamin A supplementation prior to HSCT in pediatric patients had no clinical benefit in protecting GI mucosal integrity.
Contribution of calcium in drinking water from a South American country to dietary calcium intake
Authors: Cormick Gabriela , Lombarte Mercedes , Minckas Nicole , Porta Andrés , Rigalli Alfredo , Belizán Jose M. , Matamoros Natalia , Lupo Maela 
Published: October 2020

Abstract
Objective
To describe the calcium concentration of tap and bottled waters from Argentina and to estimate the contribution of drinking water to calcium recommendations.

Results
Calcium concentrations provided by water authorities ranged from 6 to 105 mg/L. The mean calcium level of samples analysed at the Laboratorio de Ingeniería Sanitaria, National University of La Plata was 15.8 (SD ± 13.2) mg/L and at the Bone Biology Laboratory of the National University of Rosario was 13.1 (± 10.0) mg/L. Calcium values of samples from supply systems and private wells was similar. Most bottled waters had calcium levels well below 50 mg/L. The intake of one litre of drinking water from Argentina could represent in average between 1.2 and 8.0% of the calcium daily values for an adult.
TWIST1 DNA methylation is a cell marker of airway and parenchymal lung fibroblasts that are differentially methylated in asthma
Authors: Clifford Rachel L. , Yang Chen Xi , Fishbane Nick , Patel Jamie , MacIsaac Julia L. , McEwen Lisa M. , May Sean T. , Castellanos-Uribe Marcos , Nair Parameswaran , Obeidat Ma’en , Kobor Michael S. , Knox Alan J. , Hackett Tillie-Louise 
Published: October 2020

Abstract
Background
Mesenchymal fibroblasts are ubiquitous cells that maintain the extracellular matrix of organs. Within the lung, airway and parenchymal fibroblasts are crucial for lung development and are altered with disease, but it has been difficult to understand their roles due to the lack of distinct molecular markers. We studied genome-wide DNA methylation and gene expression in airway and parenchymal lung fibroblasts from healthy and asthmatic donors, to identify a robust cell marker and to determine if these cells are molecularly distinct in asthma.

Results
Airway (N = 8) and parenchymal (N = 15) lung fibroblasts from healthy individuals differed in the expression of 158 genes, and DNA methylation of 3936 CpGs (Bonferroni adjusted p value < 0.05). Differential DNA methylation between cell types was associated with differential expression of 42 genes, but no single DNA methylation CpG feature (location, effect size, number) defined the interaction. Replication of gene expression and DNA methylation in a second cohort identified TWIST1 gene expression, DNA methylation and protein expression as a cell marker of airway and parenchymal lung fibroblasts, with DNA methylation having 100% predictive discriminatory power. DNA methylation was differentially altered in parenchymal (112 regions) and airway fibroblasts (17 regions) with asthmatic status, with no overlap between regions.

Conclusions
Differential methylation of TWIST1 is a robust cell marker of airway and parenchymal lung fibroblasts. Airway and parenchymal fibroblast DNA methylation are differentially altered in individuals with asthma, and the role of both cell types should be considered in the pathogenesis of asthma.
Cerebrospinal fluid A beta 1–40 peptides increase in Alzheimer’s disease and are highly correlated with phospho-tau in control individuals
Authors: Lehmann Sylvain , Dumurgier Julien , Ayrignac Xavier , Marelli Cecilia , Alcolea Daniel , Ormaechea Juan Fortea , Thouvenot Eric , Delaby Constance , Hirtz Christophe , Vialaret Jérôme , Ginestet Nelly , Bouaziz-Amar Elodie , Laplanche Jean-Louis , Labauge Pierre , Paquet Claire , Lleo Alberto , Gabelle Audrey ,   for the Alzheimer’s Disease Neuroimaging Initiative (ADNI)
Published: October 2020

Abstract
Background
Amyloid pathology, which is one of the characteristics of Alzheimer’s disease (AD), results from altered metabolism of the beta-amyloid (Aβ) peptide in terms of synthesis, clearance, or aggregation. A decrease in cerebrospinal fluid (CSF) level Aβ1–42 is evident in AD, and the CSF ratio Aβ42/Aβ40 has recently been identified as one of the most reliable diagnostic biomarkers of amyloid pathology. Variations in inter-individual levels of Aβ1–40 in the CSF have been observed in the past, but their origins remain unclear. In addition, the variation of Aβ40 in the context of AD studied in several studies has yielded conflicting results.

Methods
Here, we analyzed the levels of Aβ1–40 using multicenter data obtained on 2466 samples from six different cohorts in which CSF was collected under standardized protocols, centrifugation, and storage conditions. Tau and p-tau (181) concentrations were measured using commercially available in vitro diagnostic immunoassays. Concentrations of CSF Aβ1–42 and Aβ1–40 were measured by ELISA, xMAP technology, chemiluminescence immunoassay (CLIA), and mass spectrometry. Statistical analyses were calculated for parametric and non-parametric comparisons, linear regression, correlation, and odds ratios. The statistical tests were adjusted for the effects of covariates (age, in particular).

Results
Regardless of the analysis method used and the cohorts, a slight but significant age-independent increase in the levels of Aβ40 in CSF was observed in AD. We also found a strong positive correlation between the levels of Aβ1–40 and p-tau (181) in CSF, particularly in control patients.

Conclusions
These results indicate that an increase in the baseline level of amyloid peptides, which are associated with an increase in p-tau (181), may be a biological characteristic and possibly a risk factor for AD. Further studies will be needed to establish a causal link between increased baseline levels of Aβ40 and the development of the disease.
Data-driven graph drawing techniques with applications for conveyor systems
Authors: Göttlich Simone , Spieckermann Sven , Stauber Stephan , Storck Andrea 
Published: October 2020

AbstractThe visualization of conveyor systems in the sense of a connected graph is a challenging problem. Starting from communication data provided by the IT system, graph drawing techniques are applied to generate an appealing layout of the conveyor system. From a mathematical point of view, the key idea is to use the concept of stress majorization to minimize a stress function over the positions of the nodes in the graph. Different to the already existing literature, we have to take care of special features inspired by the real-world problems.
Nature-based early childhood education for child health, wellbeing and development: a mixed-methods systematic review protocol
Authors: Johnstone Avril , McCrorie Paul , Cordovil Rita , Fjørtoft Ingunn , Iivonen Susanna , Jidovtseff Boris , Lopes Frederico , Reilly John J. , Thomson Hilary , Wells Valerie , Martin Anne 
Published: October 2020

AbstractBackgroundSeveral systematic reviews have reviewed the evidence relating to nature on aspects of children and adolescent’s health and wellbeing; however, none have looked at the associations or effectiveness of attending nature-based early childhood education (ECE). The main objective is to systematically review and synthesise the evidence to determine if nature-based ECE enhances children’s health, wellbeing and development.MethodsWe will search the following electronic databases (from inception onwards): MEDLINE, Scopus, PsycINFO, ERIC, SportDiscus, Australian Education Index, British Education Index, Child Development and Adolescent studies, and Applied Social Sciences Index and Abstracts. Grey literature will be identified searching dissertations and reports (e.g. Open Grey, Dissertations Theses Database [ProQuest], and Google Scholar). All types of studies (quantitative and qualitative) conducted in children (aged 2–7 years old) attending ECE who had not started education at primary or elementary school will be included. The exposure of interest will be nature-based ECE settings that integrate nature into their philosophy and/or curriculum and environment. The outcomes of interest will be all aspects of the child’s physical, cognitive, social and emotional health wellbeing and development. Two reviewers will independently screen full-text articles. The study methodological quality (or bias) will be appraised using appropriate tools. If feasible, a meta-analysis will be conducted using a random-effect model for studies similar in exposure and outcome. Where studies cannot be included in a meta-analysis, findings will be summarised based on the effect directions and a thematic analysis will be conducted for qualitative studies.DiscussionThis systematic review will capture the state of the current literature on nature-based ECE for child health, wellbeing and development. The results of this study will be of interest to multiple audiences (including researchers and policy makers). Results will be published in a peer-reviewed journal. Gaps for future research will be identified and discussed.Systematic review registrationPROSPERO CRD42019152582
Joint optimization strategy for QoE-aware encrypted video caching and content distributing in multi-edge collaborative computing environment
Authors: Liu Zhi , Qiao Bo , Fang Kui 
Published: October 2020

AbstractThe video request service of users in 5G network will explode, and adaptive bit rate technology can provide users with reliable video response. Placing video resources on edge servers close to users can overcome the problem of excessive network load similar to traditional centralized cloud platform solutions. Moreover, multiple edge servers can provide caching and transcoding support by collaboration mechanisms, which further improves users’ Quality of Experience (QoE). However, the design difficulty of video caching and content distribution strategies is increased due to the diversity of collaboration mechanisms and the competition between local and collaborative services of edge servers for computing and storage resources. In order to solve this problem, video cache and content distribution problem is modeled as random integer programming problem in the multi-edge server at most two-hop collaboration scenario. In order to improve the security of video data transmission, the video stream is encrypted using an encryption algorithm based on Logistic chaotic-Quantum-dot Cellular Automata (QCA). For improving the efficiency of solving integer programming problems, this paper uses a pyramid intelligent evolution algorithm based on optimal cooperation strategy to solve this problem. Simulation experiments show that our proposed method can obtain higher QoE value compared with several newer methods. In addition, the average access delay of proposed method is shortened by more than 27.98%, which verifies its reliability.
Exploring the influencing factors of continuous crop residue supply: from the perspective of a sustainable and bioenergy-oriented crop cultivation
Authors: Xu Xin Long , Chen Hsing Hung , Li Yi 
Published: October 2020

Abstract
Background
Crop residues are an important raw material for bioenergy. There is, however, obvious seasonality in crop harvests, and thus, a continuous supply of crop residues and its respective influencing factors should be investigated.

Methods
In this paper, the impacts of geographical climatic conditions, characteristics of crops and the macroeconomic status exerted on the continuity of crop residue supply were analysed. Likewise, the effects of various factors on the supply of crop residues were examined.

Results
The results indicate that planting scale, crop diversity, climatic conditions, and topography have a significantly positive impact on a continuous crop residue supply, whereas the planting structure, temperature square term, energy pressure, and the economic development level have a significantly negative one. Finally, a regression-based decomposition method was used to measure the contribution rate of each variable onto the inequalities in the continuous supply of crop residues, which confirms that the impact of characteristics of crops on its continuous supply was the highest. The economic development level is the most important factor that affects the inequalities in the continuity of crop residue supply.

Conclusions
The above results were achieved by using different measurement methods, and based upon the findings obtained, this paper proposes policies and suggestions for ensuring sustainable and bioenergy-oriented crop cultivation.
Reliability and validity of the functioning assessment short test for older adults with bipolar disorder (FAST-O)
Authors: Orhan Melis , Korten Nicole , Kupka Ralph , van Oppen Patricia , Stek Max , Vieta Eduard , Schouws Sigfried , van Ballegooijen Wouter , Dols Annemiek 
Published: October 2020

Abstract
Background
Many frequently used instruments fail to assess psychosocial functioning in patients with bipolar disorder. The Functioning Assessment Short Test (FAST) was developed in order to tackle this problem and to assess the main functioning problems experienced by patients with bipolar disorder. However, the original FAST is not fully applicable in older adults due to the domain of occupational functioning. The aim of our study was to validate an adapted version for Older adults (FAST-O) in a group of older adults with bipolar disorder (OABD).

Methods
88 patients aged 50 years and over diagnosed with bipolar disorder were included. We adapted the items in the area of “work-related functioning” of the FAST into items assessing “societal functioning”. Several measurements were conducted in order to analyse the psychometric qualities of the FAST-O (confirmatory factor analysis for internal structure, Cronbach’s alpha for internal consistency, Spearman’s rho for concurrent validity, Mann–Whitney U test for discriminant validity).

Results
Mean age in the study sample was 65.3 (SD = 7.5) and 57.3% was female. The internal structure was most similar to the internal structure of the original FAST. The internal consistency was excellent (Cronbach’s alpha = .93). The concurrent validity when correlated with the Social and Occupational Functioning Assessment Scale was low, but significant. The FAST-O was also able to distinguish between euthymic and symptomatic OABD patients.

Conclusions
The FAST-O has strong psychometric qualities. Based on our results, we can conclude that the FAST-O is a short, efficient solution in order to replace global rating scales or extensive test batteries in order to assess daily functioning of older psychiatric patients in a valid and reliable manner.
An open-access stress magnitude database for Germany and adjacent regions
Authors: Morawietz Sophia , Heidbach Oliver , Reiter Karsten , Ziegler Moritz , Rajabi Mojtaba , Zimmermann Günter , Müller Birgit , Tingay Mark 
Published: October 2020

AbstractKnowledge of the crustal stress state is important for the assessment of subsurface stability. In particular, stress magnitudes are essential for the calibration of geomechanical models that estimate a continuous description of the 3-D stress field from pointwise and incomplete stress data. Well established is the World Stress Map Project, a global and publicly available database for stress orientations, but for stress magnitude data only local data collections are available. Herein, we present the first comprehensive and open-access stress magnitude database for Germany and adjacent regions, consisting of 568 data records. In addition, we introduce a quality ranking scheme for stress magnitude data for the first time.
Future projection of greenhouse gas emissions due to permafrost degradation using a simple numerical scheme with a global land surface model
Authors: Yokohata Tokuta , Saito Kazuyuki , Ito Akihiko , Ohno Hiroshi , Tanaka Katsumasa , Hajima Tomohiro , Iwahana Go 
Published: October 2020

AbstractThe Yedoma layer, a permafrost layer containing a massive amount of underground ice in the Arctic regions, is reported to be rapidly thawing. In this study, we develop the Permafrost Degradation and Greenhouse gasses Emission Model (PDGEM), which describes the thawing of the Arctic permafrost including the Yedoma layer due to climate change and the greenhouse gas (GHG) emissions. The PDGEM includes the processes by which high-concentration GHGs (CO2and CH4) contained in the pores of the Yedoma layer are released directly by dynamic degradation, as well as the processes by which GHGs are released by the decomposition of organic matter in the Yedoma layer and other permafrost. Our model simulations show that the total GHG emissions from permafrost degradation in the RCP8.5 scenario was estimated to be 31-63 PgC for CO2and 1261-2821 TgCH4for CH4(68thpercentile of the perturbed model simulations, corresponding to a global average surface air temperature change of 0.05–0.11 °C), and 14-28 PgC for CO2and 618-1341 TgCH4for CH4(0.03–0.07 °C) in the RCP2.6 scenario. GHG emissions resulting from the dynamic degradation of the Yedoma layer were estimated to be less than 1% of the total emissions from the permafrost in both scenarios, possibly because of the small area ratio of the Yedoma layer. An advantage of PDGEM is that geographical distributions of GHG emissions can be estimated by combining a state-of-the-art land surface model featuring detailed physical processes with a GHG release model using a simple scheme, enabling us to consider a broad range of uncertainty regarding model parameters. In regions with large GHG emissions due to permafrost thawing, it may be possible to help reduce GHG emissions by taking measures such as restraining land development.
Lost in translation: barriers and progress in harnessing basic medical science into community practice in Indonesia
Authors: Diptyanusa Ajib , Hasanbasri Mubasysyir 
Published: October 2020

AbstractTranslational research carries over fundamental laboratory research findings into clinical and community settings in order to ‘translate’ the findings into practice. In addition to its potential in accelerating the time of basic discoveries to be delivered into the population, translational studies also provide opportunities for interdisciplinary collaborations in identifying knowledge gaps. However, several issues hindering the advancement of translational research studies have risen in developing countries, such as limited funding, insufficient research evaluation and recognition, absence of government model or consortium, and insufficient communication among researchers and stakeholders. While the original concept of the ‘bench to bedside’ has been known for years, the practice of bilateral flow from bench to bedside, and back to the bench was found lacking. Lack of interactions and involvement of the clinicians, community and resources further deplete translational ability of the research itself. Without understanding the priorities and the environment in which the decision makers work, specific research aims needed by the communities may be failed to be formulated and may result in devaluation of research by formulating similar key questions repetitively. The current commentary aimed to highlight the importance of connecting population health systems, basic demands, and academic institutions, to own the issues, to address the issues, and to translate research findings.