bluuebunny/crossref_metadata_2025_split huggingface dataset:
APOBEC3A Catalyzes Mutation and Drives CarcinogenesisIn Vivo
Authors: Law Emily K. , Levin-Klein Rena , Jarvis Matthew C. , Kim Hyoung , Argyris Prokopios P. , Carpenter Michael A. , Starrett Gabriel J. , Larson Lindsay K. , Burns Michael B. , Vogel Rachel I. , Stavrou Spyridon , Aguilera Alexya N. , Wagner Sandra , Largaespada David A. , Starr Timothy K. , Ross Susan R. , Harris Reuben S. 
Published: December 2019

SUMMARYThe APOBEC3 family of antiviral DNA cytosine deaminases is implicated as the second largest source of mutation in cancer. This mutational process may be a causal driver or inconsequential passenger to the overall tumor phenotype. We show that human APOBEC3A expression in murine colon and liver tissues increases tumorigenesis. All other APOBEC3 family members including APOBEC3B did not as strongly promote liver tumor formation. DNA sequences from APOBEC3A-expressing animals display hallmark APOBEC signature mutations in TCA/T motifs. Bioinformatic comparisons of the observed APOBEC3A mutation signature in murine tumors, previously reported APOBEC3A and APOBEC3B mutation signatures in yeast, and reanalyzed APOBEC mutation signatures in human tumor data sets support cause-and-effect relationships for APOBEC3A-catalyzed deamination and mutagenesis in driving multiple human cancers.
Citrullination regulates wound responses and tissue regeneration in zebrafish
Authors: Golenberg Netta , Squirrell Jayne M. , Bennin David A. , Rindy Julie , Pistono Paige E. , Eliceiri Kevin W. , Shelef Miriam A. , Kang Junsu , Huttenlocher Anna 
Published: December 2019

AbstractCalcium signaling is an important early step in wound healing, yet how these early signals promote regeneration remains unclear. Peptidylarginine deiminases (PADs), a family of calcium-dependent enzymes, catalyze citrullination, a post-translational modification that alters protein function and has been implicated in autoimmune diseases. We generated a mutation in the single zebrafish ancestralpadgene,padi2,resulting in a loss of detectable calcium-dependent citrullination. Thepadi2mutants exhibit impaired resolution of inflammation and regeneration after caudal fin transection. Further, we identified a new subpopulation of cells displaying citrullinated histones within the notochord bead following tissue injury. Citrullination of histones in this region was absent and wound-induced proliferation was perturbed in Padi2-deficient larvae. Taken together, our results show that Padi2 is required for the citrullination of histones within a group of cells in the notochord bead, and for promoting wound-induced proliferation required for efficient regeneration. These findings identify Padi2 as a potential intermediary between early calcium signaling and subsequent tissue regeneration.SummaryGolenberg et al. developed a citrullination-deficient zebrafish and demonstrated a role for Padi2 in fin wound responses and regeneration. This work identified a distinct population of cells within the regenerative notochord bead that exhibited wound-induced histone citrullination.
Differential abilities to engage inaccessible chromatin diversify vertebrate HOX binding patterns
Authors: Bulajić Milica , Srivastava Divyanshi , Dasen Jeremy S , Wichterle Hynek , Mahony Shaun , Mazzoni Esteban O 
Published: December 2019

ABSTRACTWhileHoxgenes encode for conserved transcription factors (TFs), they are further divided into anterior, central, and posterior groups based on their DNA-binding domain similarity. The posteriorHoxgroup expanded in the deuterostome clade and patterns caudal and distal structures. We aim to address how similar HOX TFs diverge to induce different positional identities. We studied HOX TF DNA-binding and regulatory activity during anin vitromotor neuron differentiation system that recapitulates embryonic development. We find diversity in the genomic binding profiles of different HOX TFs, even among the posterior group paralogs that share similar DNA binding domains. These differences in genomic binding are explained by differing abilities to bind to previously inaccessible sites. For example, the posterior group HOXC9 has a greater ability to bind occluded sites than the posterior HOXC10, producing different binding patterns and driving differential gene expression programs. From these results, we propose that the differential abilities of posterior HOX TFs to bind to previously inaccessible chromatin drive patterning diversification.
Mitochondrial Proteome of Affected Neurons in a Mouse Model of Leigh Syndrome
Authors: Gella Alejandro , Prada-Dacasa Patricia , Carrascal Montserrat , González-Torres Melania , Abian Joaquin , Sanz Elisenda , Quintana Albert 
Published: December 2019

AbstractDefects in mitochondrial function lead to severe neuromuscular orphan pathologies known as mitochondrial disease. Among them, Leigh Syndrome is the most common pediatric presentation, characterized by symmetrical brain lesions, hypotonia, motor and respiratory deficits, and premature death. Mitochondrial diseases are characterized by a marked anatomical and cellular specificity. However, the molecular determinants for this susceptibility are currently unknown, hindering the efforts to find an effective treatment. Due to the complex crosstalk between mitochondria and their supporting cell, strategies to assess the underlying alterations in affected cell types in the context of mitochondrial dysfunction are critical. Here, we developed a novel virus-based tool, the AAV-mitoTag viral vector, to isolate mitochondria from genetically-defined cell types. Administration of the AAV-mitoTag in the vestibular neurons of a mouse model of Leigh Syndrome lacking the complex I subunitNdufs4allowed us to assess the proteome and acetylome of susceptible neurons in a well characterized model recapitulating the human disease. Our results show a marked reduction of complex-I N-module subunit abundance and an increase in the levels of the assembly factor NDUFA2. Transiently-associated non-mitochondrial proteins such as PKCδ, and the complement subcomponent C1Q were also increased inNdufs4-deficientmitochondria. Furthermore, lack ofNdufs4induced pyruvate dehydrogenase (PDH) subunit hyperacetylation, leading to decreased PDH activity. We provide novel insight on the pathways involved in mitochondrial disease, which could underlie potential therapeutic approaches for these pathologies.
Polar pattern formation induced by contact following locomotion in a multicellular system
Authors: Hayakawa Masayuki , Hiraiwa Tetsuya , Wada Yuko , Kuwayama Hidekazu , Shibata Tatsuo 
Published: December 2019

AbstractBiophysical mechanisms underlying collective cell migration of eukaryotic cells have been studied extensively in recent years. One paradigm that induces cells to correlate their motions is contact inhibition of locomotion, by which cells migrating away from the contact site. Here, we report that tail-following behavior at the contact site, termed contact following locomotion (CFL), can induce a non-trivial collective behavior in migrating cells. We show the emergence of a traveling band showing polar order in a mutantDictyosteliumcell that lacks chemotactic activity. The traveling band is dynamic in the sense that it continuously assembled at the front of the band and disassembled at the back. A mutant cell lacking cell adhesion molecule TgrB1 did not show both the traveling band formation and CFL. We thus conclude that CFL is the cell-cell interaction underlying the traveling band formation. We then develop an agent-based simulation with CFL, which shows the role of CFL in the formation of traveling band. We further show that the polar order phase consists of subpopulations that exhibit characteristic transversal motions with respect to the direction of band propagation. These findings describe a novel mechanism of collective cell migration involving cell–cell interactions capable of inducing traveling band with polar order.
Implementation of the CLiP database
Authors: Lee Kenneth Ken Siong , Silim Umi Adzlin 
Published: December 2019

Purpose
The purpose of this paper is to review the findings from an audit of the implementation of a consultation-liaison psychiatry (CLiP) database in all inpatients referred to a CLiP service at the largest hospital in Malaysia with the aim of improving the quality CLiP services.

Design/methodology/approach
All inpatient referrals to the CLiP team were recorded over a three-month period and compared to previous audit data from 2017. Four audit standards were assessed: the reporting of referrals, timeliness of response indication of reason for referral and presence of a management plan.

Findings
The compliance of reporting using the CLiP form was 70.1 per cent compared to 28 per cent in the audit data from 2017 after interventions were conducted. Analysis of the completed CLiP form reveals that 89 per cent of referrals were seen within the same working day. All referrals included the reason for referral. The most common reason for referral was for depressive disorders, but post-assessment, delirium was the most common diagnosis. In total, 87.8 per cent satisfied the audit criteria for a completed written care plan.

Originality/value
Specialised CLiP services are relatively new in Malaysia and this is the first paper to examine the quality of such services in the country. Interventions were effective in improving the compliance of reporting using the CLiP database. The findings suggest that the CLiP services are on par with international audit standards. Furthermore, data from this clinical audit can serve as a benchmark for the development of national operating policies in similar settings.
Objective measures of workload in healthcare: a narrative review
Authors: Fishbein Daniela , Nambiar Siddhartha , McKenzie Kendall , Mayorga Maria , Miller Kristen , Tran Kevin , Schubel Laura , Agor Joseph , Kim Tracy , Capan Muge 
Published: December 2019

PurposeWorkload is a critical concept in the evaluation of performance and quality in healthcare systems, but its definition relies on the perspective (e.g. individual clinician-level vs unit-level workload) and type of available metrics (e.g. objective vs subjective measures). The purpose of this paper is to provide an overview of objective measures of workload associated with direct care delivery in tertiary healthcare settings, with a focus on measures that can be obtained from electronic records to inform operationalization of workload measurement.Design/methodology/approachRelevant papers published between January 2008 and July 2018 were identified through a search in Pubmed and Compendex databases using the Sample, Phenomenon of Interest, Design, Evaluation, Research Type framework. Identified measures were classified into four levels of workload: task, patient, clinician and unit.FindingsOf 30 papers reviewed, 9 used task-level metrics, 14 used patient-level metrics, 7 used clinician-level metrics and 20 used unit-level metrics. Key objective measures of workload include: patient turnover (n=9), volume of patients (n=6), acuity (n=6), nurse-to-patient ratios (n=5) and direct care time (n=5). Several methods for operationalization of these metrics into measurement tools were identified.Originality/valueThis review highlights the key objective workload measures available in electronic records that can be utilized to develop an operational approach for quantifying workload. Insights gained from this review can inform the design of processes to track workload and mitigate the effects of increased workload on patient outcomes and clinician performance.
Identifying and evaluating synergies of Lean Six Sigma and knowledge management in deliberately interlocking application
Authors: Strubelt Henning , Mollenhauer Felix 
Published: December 2019

Purpose
The purpose of this paper is to identify and evaluate potential synergies between the two management approaches Lean Six Sigma (LSS) and knowledge management. Although a strong interaction between them is suspected, there is only very little academic research on their possible interrelation available. This paper aims to close this research gap.

Design/methodology/approach
Based on a comprehensive literature review and a comparison of LSS and knowledge management two hypotheses on their interlocking application are formulated, discussed and evaluated.

Findings
Knowledge management supports and improves the application of LSS in various ways. In particular, the deliberate integration of communities of practice, information and communication technologies, and feedback and “lessons learned” sessions can develop potentially positive synergy effects and contribute positively to the success of LSS projects. In contrast, LSS turns out to be an inadequate methodology to significantly improve knowledge management, mainly due to the imprecise measurability of knowledge management processes.

Research limitations/implications
The findings are based on a literature review and are not supported by empirical evidence. Therefore, empirical research in this field is suggested.

Practical implications
Organizations using LSS could enhance their performance by integrating instruments of knowledge management, whereas they should critically examine LSS as a methodology for improving knowledge management.

Originality/value
Considering the existing research gap in this field, this paper encourages organizations using LSS to reflect on if and how they could integrate instruments of knowledge management into their practice to achieve better LSS results.
Financing accumulation for start-up capital
Authors: Daovisan Hanvedes , Chamaratana Thanapauge 
Published: December 2019

Purpose
The purpose of this paper is to understand the sources of financing accumulation that women entrepreneurs of family businesses use for start-up capital in the garment sector of the Lao People’s Democratic Republic (Lao PDR).

Design/methodology/approach
This study presents insights gleaned from a qualitative case study into the ways in which women in Lao PDR finance their family businesses in the start-up phase. The authors conducted 36 in-depth interviews – the study used this purposive sample in each of its five rounds of data collection. The data were collected between December 2018 and April 2019 and were analysed by conducting a content analysis assisted by the software programme ATLAS.ti.

Findings
The results, though highly case specific, show Lao women’s ability to: accrue their experience, apply their knowledge, engage in self-employment, support their families and aspire to become entrepreneurs. The findings clearly illustrate that women are opportunity and necessity driven, can accumulate income, possess savings behaviour, can manage working capital, investment and accounting and have access to finance (loan and debt) and thus have the potential to become successful entrepreneurs.

Originality/value
By contextualizing women’s entrepreneurial practices, the paper contributes to an understanding of the sources of financing accumulation used for start-up capital in Vientiane, Lao PDR. Theoretically, the paper extends the knowledge of women entrepreneurs seeking the optimal stock of finance which has the potential to drive family business success.
Examining the mediating role of innovativeness in the link between core competencies and SME performance
Authors: Ng Hee Song , Kee Daisy Mui Hung , Ramayah T. 
Published: December 2019

PurposeThe purpose of this paper is to evaluate the effect of core competencies, namely, transformational leadership (TFL), entrepreneurial competence and technical competence on financial performance through the mediation effect of innovativeness, among owner-managed small and medium-sized enterprises (SMEs) in developing countries.Design/methodology/approachA research model was developed to test nine research hypotheses. Self-report questionnaires designed for this study were sent to SME owner-managers in Malaysia. A total of 178 completed questionnaires were successfully collected. SPSS and SmartPLS were used to perform the data analysis to test the measurement model and structural model.FindingsThis paper provides empirical evidence that behavioural innovativeness mediates the relationship between TFL and financial performance, product innovativeness mediates the relationships among entrepreneurial competence, technical competence and financial performance and process innovativeness mediates the relationship between technical competence and financial performance.Research limitations/implicationsThe findings of this study are potentially limited by perceptual measures, cross-sectional data and the risk of response bias from a single informant.Practical implicationsOwner-managed SMEs can focus on developing the core competencies to achieve financial performance through innovative products, processes and behaviours. Policymakers and practitioners can gain fresh insights into the complexity of sustaining the business activities and financial performance of SMEs through the core competencies and innovativeness.Originality/valueThe extant literature has revealed that entrepreneurship, leadership, expertise and innovativeness are considered key factors in promoting financial performance, yet little is known about the combined effects of the core competencies on financial performance through innovativeness for owner-managed SMEs in the context of a developing country. The study makes an important contribution to filling this research gap.
Identification of barriers in the implementation of AMTs in the SMEs of northern India using AHP–TOPSIS approach
Authors: Kaushik Arishu , Singh Doordarshi 
Published: December 2019

Purpose
The purpose of this paper is to find out and highlight the major influential barriers in the implementation of advanced manufacturing technologies (AMTs) in small and medium enterprises (SMEs) of Northern India. The major barriers in the implementation of AMTs in manufacturing industries of Northern India have been critically assessed in this paper.

Design/methodology/approach
An ample and reasonable number of small- and medium-scale manufacturing industries of northern India have been surveyed with an aim to find out the barriers in the implementation of AMTs. On the basis of data collected, AHP–TOPSIS method was applied in order to measure the weightage of each barrier in a simple mathematical form.

Findings
High cost of AMTs, lack of appropriate financial resources and current processes or procedures are the major barriers that cause hindrance in the path of implementation of AMTs in SMEs.

Research limitations/implications
This investigation was based on the survey followed by judgments of experts in industry and academia; other approaches such as PROMETHEE, WPM, VIKOR, etc., can be applied for investigation. Also, the study can be carried out in different region(s) and parts of the country.

Practical implications
This paper can be helpful in many ways to the management or industrialists of various nations who are on the same path or will follow soon.

Originality/value
SMEs need to address the findings of this research in order to overcome the barriers and successfully implement the AMTs. A model for successful implementation of AMTs by overcoming the barriers has been suggested.
The effect of evictions on accidental drug and alcohol mortality
Authors: Bradford Ashley C. , Bradford W. David 
Published: December 2019

AbstractObjectiveTo assess the relationship between county‐level eviction rates and drug and alcohol mortality rates.Data SourcesEviction rates from 2003 to 2016 provided by the Princeton University Eviction Lab were merged with Multiple Cause‐of‐Death Mortality Files and aggregated to the county‐year level.Study DesignAll opioid (prescription and heroin), cocaine, psychostimulant, benzodiazepine, antidepressant, and alcohol poisoning–related deaths per 100 000 people, eviction rates, and socioeconomic indicators were merged at the county‐year level from 2003 to 2016. We estimated a series of mortality rate models with county and year fixed effects and used a control function (2SRI) method to adjust for the endogeneity of eviction rates.Data Collection/Extraction MethodsWe matched retrospectively collected datasets.Principal FindingsHigher levels of eviction rates were consistently associated with higher rates of mortality across six of nine substance categories studied when all counties were combined. Subanalysis by USDA population density measures indicated this positive association was almost entirely driven by urban counties; few systematic associations between the eviction rate levels and mortality were observed for suburban or rural counties.ConclusionsRisk of eviction appears to exacerbate the current “deaths of despair” crisis associated with substance use. Proposed changes to Housing and Urban Development policy that are expected to substantially increase the risk of eviction may worsen an already‐acute mortality crisis.
MiR‐148b suppressed non‐small cell lung cancer progression via inhibiting ALCAM through the NF‐κB signaling pathway
Authors: Jiang Zhe , Zhang JingWen , Chen FuHui , Sun Yajiao 
Published: December 2019

BackgroundNon‐small cell lung cancer (NSCLC) is the leading cause of cancer mortality worldwide. MiRNAs are recognized as important molecules in cancer biology. The aim of the study was to identify a novel biomarker miR‐148b and its mechanism in the modulation of NSCLC progression.MethodsThe expressional level of miR‐148b was analyzed by RT‐PCR. The effect of miR‐4317 on proliferation was evaluated through 3‐(4,5‐Dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2Htetrazolium bromide (MTT) assay. The effect of miR‐148b on the metastasis of NSCLC was detected through transwell assays. The verification of the target of miR‐148b was assessed by TargetScan and dual‐luciferase reporter assay. The related proteins in this study were analyzed by western blot.ResultsOur findings confirmed that miR‐148b was decreased in NSCLC and NSCLC patients with lower expression exhibited poorer overall survival (OS). Increasing miR‐148b significantly repressed proliferation, invasion and migration. More importantly, activated leukocyte cell adhesion molecule (ALCAM) was determined as the direct target of miR‐148b, and reintroduction of ALCAM attenuated miR‐148b effect on the progress of NSCLC. In addition, NF‐κB signaling pathway was modulated by miR‐148b/ALCAM axis.ConclusionsOur results indicated that miR‐148b is able to suppress NSCLC growth and metastasis via targeting ALCAM through the NF‐κB pathway. These findings provided new evidence that miR‐148b serves as a potential biomarker and novel target for NSCLC treatment.
SEEING SLAVES IN AESCHYLEAN SATYR DRAMA
Authors: UHLIG ANNA 
Published: December 2019

Abstract
This article explores the thematization of the satyrs’ proverbial slave status with specific reference to Aeschylean satyr play. A survey of the extant fragments reveals only one explicit mention of the satyrs’ slavery, suggesting a stark contrast with the relatively frequent references in the satyr plays of Sophocles and Euripides. Situating Aeschylus’ often enigmatic satyr fragments within the broader historical framework of fifth-century Athenian slavery, it is possible to see that the chorus’ servitude is nonetheless obliquely figured in many of our extant passages. At the same time, Aeschylus’ reticence around the subject of slavery in his satyric works is shown to continue a disposition already in evidence in his tragic compositions, which manifest a similarly muted discourse around lower-class enslavement.