bluuebunny/crossref_metadata_2025_split huggingface dataset:
Orbitofrontal-striatal potentiation underlies cocaine-induced hyperactivity
Authors: Bariselli Sebastiano , Miyazaki Nanami L. , Creed Meaghan C. , Kravitz Alexxai V. 
Published: August 2020

AbstractPsychomotor stimulants increase dopamine levels in the striatum and promote locomotion; however, their effects on striatal pathway function in vivo remain unclear. One model that has been proposed to account for these motor effects suggests that stimulants drive hyperactivity via activation and inhibition of direct and indirect pathway striatal neurons, respectively. Although this hypothesis is consistent with the cellular actions of dopamine receptors and received support from optogenetic and chemogenetic studies, it has been rarely tested with in vivo recordings. Here, we test this model and observe that cocaine increases the activity of both pathways in the striatum of awake mice. These changes are linked to a dopamine-dependent cocaine-induced strengthening of upstream orbitofrontal cortex (OFC) inputs to the dorsomedial striatum (DMS) in vivo. Finally, depressing OFC-DMS pathway with a high frequency stimulation protocol in awake mice over-powers the cocaine-induced potentiation of OFC-DMS pathway and attenuates the expression of locomotor sensitization, directly linking OFC-DMS potentiation to cocaine-induced hyperactivity.
Developmental potential of aneuploid human embryos cultured beyond implantation
Authors: Shahbazi Marta N. , Wang Tianren , Tao Xin , Weatherbee Bailey A. T. , Sun Li , Zhan Yiping , Keller Laura , Smith Gary D. , Pellicer Antonio , Scott Richard T. , Seli Emre , Zernicka-Goetz Magdalena 
Published: August 2020

AbstractAneuploidy, the presence of an abnormal number of chromosomes, is a major cause of early pregnancy loss in humans. Yet, the developmental consequences of specific aneuploidies remain unexplored. Here, we determine the extent of post-implantation development of human embryos bearing common aneuploidies using a recently established culture platform. We show that while trisomy 15 and trisomy 21 embryos develop similarly to euploid embryos, monosomy 21 embryos exhibit high rates of developmental arrest, and trisomy 16 embryos display a hypo-proliferation of the trophoblast, the tissue that forms the placenta. Using human trophoblast stem cells, we show that this phenotype can be mechanistically ascribed to increased levels of the cell adhesion protein E-CADHERIN, which lead to premature differentiation and cell cycle arrest. We identify three cases of mosaicism in embryos diagnosed as full aneuploid by pre-implantation genetic testing. Our results present the first detailed analysis of post-implantation development of aneuploid human embryos.
Ultrafast photoinduced band splitting and carrier dynamics in chiral tellurium nanosheets
Authors: Jnawali Giriraj , Xiang Yuan , Linser Samuel M. , Shojaei Iraj Abbasian , Wang Ruoxing , Qiu Gang , Lian Chao , Wong Bryan M. , Wu Wenzhuo , Ye Peide D. , Leng Yongsheng , Jackson Howard E. , Smith Leigh M. 
Published: August 2020

AbstractTrigonal tellurium (Te) is a chiral semiconductor that lacks both mirror and inversion symmetries, resulting in complex band structures with Weyl crossings and unique spin textures. Detailed time-resolved polarized reflectance spectroscopy is used to investigate its band structure and carrier dynamics. The polarized transient spectra reveal optical transitions between the uppermost spin-split H4 and H5 and the degenerate H6 valence bands (VB) and the lowest degenerate H6 conduction band (CB) as well as a higher energy transition at the L-point. Surprisingly, the degeneracy of the H6 CB (a proposed Weyl node) is lifted and the spin-split VB gap is reduced upon photoexcitation before relaxing to equilibrium as the carriers decay. Using ab initio density functional theory (DFT) calculations, we conclude that the dynamic band structure is caused by a photoinduced shear strain in the Te film that breaks the screw symmetry of the crystal. The band-edge anisotropy is also reflected in the hot carrier decay rate, which is a factor of two slower along the c-axis than perpendicular to it. The majority of photoexcited carriers near the band-edge are seen to recombine within 30 ps while higher lying transitions observed near 1.2 eV appear to have substantially longer lifetimes, potentially due to contributions of intervalley processes in the recombination rate. These new findings shed light on the strong correlation between photoinduced carriers and electronic structure in anisotropic crystals, which opens a potential pathway for designing novel Te-based devices that take advantage of the topological structures as well as strong spin-related properties.
PD-1 pathway regulates ILC2 metabolism and PD-1 agonist treatment ameliorates airway hyperreactivity
Authors: Helou Doumet Georges , Shafiei-Jahani Pedram , Lo Richard , Howard Emily , Hurrell Benjamin P. , Galle-Treger Lauriane , Painter Jacob D. , Lewis Gavin , Soroosh Pejman , Sharpe Arlene H. , Akbari Omid 
Published: August 2020

AbstractAllergic asthma is a leading chronic disease associated with airway hyperreactivity (AHR). Type-2 innate lymphoid cells (ILC2s) are a potent source of T-helper 2 (Th2) cytokines that promote AHR and lung inflammation. As the programmed cell death protein-1 (PD-1) inhibitory axis regulates a variety of immune responses, here we investigate PD-1 function in pulmonary ILC2s during IL-33-induced airway inflammation. PD-1 limits the viability of ILC2s and downregulates their effector functions. Additionally, PD-1 deficiency shifts ILC2 metabolism toward glycolysis, glutaminolysis and methionine catabolism. PD-1 thus acts as a metabolic checkpoint in ILC2s, affecting cellular activation and proliferation. As the blockade of PD-1 exacerbates AHR, we also develop a human PD-1 agonist and show that it can ameliorate AHR and suppresses lung inflammation in a humanized mouse model. Together, these results highlight the importance of PD-1 agonistic treatment in allergic asthma and underscore its therapeutic potential.
Transparent flexible thin-film p–n junction thermoelectric module
Authors: Wang Xizu , Suwardi Ady , Lim Siew Lay , Wei Fengxia , Xu Jianwei 
Published: August 2020

AbstractTransparent and flexible thermoelectrics has been highly sought after for future wearable devices. However, the main stumbling block to prevent its widespread adoption is the lack of p-type transparent thermoelectrics and the stringent criteria of electrical and thermal properties matching appropriately between p-legs and n-legs. This work demonstrates the fabrication of p-type PEDOT:PSS films whose optical properties, electrical conductivity, thermal conductivity, and Seebeck coefficient were engineered to perfectly match the n-type indium tin oxide (ITO) counterparts. The dense p-type PEDOT:PSS and n-type ITO thin films show a thermoelectric figure of merit of zT = 0.30 and 0.29 at 450 K, and a thermal conductivity of 0.22 and 0.32 W m−1 K−1, respectively. A flexible thermoelectric generator (TEG) module with a high transmittance of >81% in the visible wavelength range of 400–800 nm is fabricated using 10 pairs of p-type PEDOT:PSS and n-type ITO thin film legs. An ultra-high power density of 22.2 W m−2 at a temperature gradient of 80 K was observed, which is the highest power density reported for organic/hybrid-based flexible TEGs so far. Our transparent flexible thin-film p–n junction thermoelectric module with exceptionally high power generation may take a tremendous step forward towards multi-functional wearable devices.
“Groupitizing”: a strategy for numerosity estimation
Authors: Anobile Giovanni , Castaldi Elisa , Moscoso Paula A. Maldonado , Burr David C. , Arrighi Roberto 
Published: August 2020

AbstractPrevious work has shown that when arrays of objects are grouped within clusters, participants can enumerate their numerosity more rapidly than when objects are randomly scattered, a phenomenon termed “groupitizing”. Importantly, the magnitude of the grouping advantage correlates with math abilities in children. Here we show that sensory precision of numerosity estimation is also improved when grouping cues are available, by up to 20%. The grouping can be induced by color and/or spatial proximity, and occurs in temporal sequences as well as spatial arrays. The improvement is strongest for participants with the highest thresholds in the random, ungrouped conditions. Taken together with previous research, our data suggest that measurements correlations between numerosity estimation and formal math skills may be driven by grouping strategies, which require a minimal level of basic arithmetic.
Long-term and longitudinal nutrient stoichiometry changes in oligotrophic cascade reservoirs with trout cage aquaculture
Authors: Miao Shiyu , Jian Shenglong , Liu Yang , Li Changzhong , Guan Hongtao , Li Kemao , Wang Guojie , Wang Zhenji 
Published: August 2020

AbstractThe potential nutrient stoichiometry changes caused by trout cage aquaculture is concerned especially in oligotrophic waters. Long-term total nitrogen (N), total phosphorus (P) and N:P ratio changes in 6 cascade reservoirs with rainbow trout cage aquaculture in the oligotrophic upstream Yellow River (UYR) were studied from 2013 to 2017 in this paper. The 5-year monitoring results showed that N, P and N:P ratio levels showed no obvious long-term changes in high-altitude oligotrophic waters with rainbow trout cage aquaculture. No obvious longitudinal N, P and N:P ratio level changes were observed in the 6 cascade reservoirs from upstream Longyangxia Reservoir (LYR) to downstream Jishixia Reservoir (JSR). The increased N and P resulting from the cage aquaculture accounted only for 1.74% and 5.2% of the natural N and P levels, respectively, with a fish production of 10,000 tonnes. The upstream Yellow River remained oligotrophic and phosphorus-limited. Results in this study proved that trout cage aquaculture do not necessarily cause nitrogen, phosphorus and N:P ratio changes even in oligotrophic waters. Phosphorus should be considered first when identifying priority nitrogen and phosphorus sources and the corresponding control measures in waters with high N:P ratio.
Regulated expression and function of the GABAB receptor in human pancreatic beta cell line and islets
Authors: Rachdi Latif , Maugein Alicia , Pechberty Severine , Armanet Mathieu , Hamroune Juliette , Ravassard Philippe , Marullo Stefano , Albagli Olivier , Scharfmann Raphael 
Published: August 2020

AbstractG protein-coupled receptors are seven transmembrane signaling molecules that are involved in a wide variety of physiological processes. They constitute a large protein family of receptors with almost 300 members detected in human pancreatic islet preparations. However, the functional role of these receptors in pancreatic islets is unknown in most cases. We generated a new stable human beta cell line from neonatal pancreas. This cell line, named ECN90 expresses both subunits (GABBR1 and GABBR2) of the metabotropic GABAB receptor compared to human islet. In ECN90 cells, baclofen, a specific GABAB receptor agonist, inhibits cAMP signaling causing decreased expression of beta cell-specific genes such as MAFA and PCSK1, and reduced insulin secretion. We next demonstrated that in primary human islets, GABBR2 mRNA expression is strongly induced under cAMP signaling, while GABBR1 mRNA is constitutively expressed. We also found that induction and activation of the GABAB receptor in human islets modulates insulin secretion.
Pavlovian bias in Parkinson’s disease: an objective marker of impulsivity that modulates with deep brain stimulation
Authors: Eisinger Robert S. , Scott Bonnie M. , Le Anh , Ponce Elena M. Torres , Lanese Joseph , Hundley Christopher , Nelson Brawn , Ravy Tasmeah , Lopes Janine , Thompson Sable , Sathish Sneha , O’Connell Rebecca L. , Okun Michael S. , Bowers Dawn , Gunduz Aysegul 
Published: August 2020

AbstractImpulsivity is a common symptom in Parkinson’s disease (PD). Adaptive behavior is influenced by prepotent action-reward and inaction-avoid loss Pavlovian biases. We aimed to assess the hypothesis that impulsivity in PD is associated with Pavlovian bias, and to assess whether dopaminergic medications and deep brain stimulation (DBS) influence Pavlovian bias. A PD DBS cohort (N = 37) completed a reward-based Go/No-Go task and bias measures were calculated. This DBS cohort completed the task under three conditions: on-med/pre-DBS, off-med/off-DBS, and on-med/on-DBS. Participants also completed self-reported measures of impulsivity. Dopaminergic medication was associated with lower action-reward bias while DBS was associated with higher action-reward bias. Impulsivity was associated with higher action-reward bias but not inaction-avoid loss bias. We furthermore replicated this association in an independent, non-DBS PD cohort (N = 88). Overall we establish an objective behavioral marker of impulsivity and show that DBS affects impulsivity by amplifying automated responding. Our results point to the importance of reward rather than punishment avoidance in driving impulsive behaviors. This work provides insight into the pathophysiological underpinnings of impulsivity and especially medication and DBS-associated impulsivity in PD.
Nonlinear model of cascade failure in weighted complex networks considering overloaded edges
Authors: Chen Chao-Yang , Zhao Yang , Gao Jianxi , Stanley Harry Eugene 
Published: August 2020

AbstractConsidering the elasticity of the real networks, the components in the network have a redundant capacity against the load, such as power grids, traffic networks and so on. Moreover, the interaction strength between nodes is often different. This paper proposes a novel nonlinear model of cascade failure in weighted complex networks considering overloaded edges to describe the redundant capacity for edges and capture the interaction strength of nodes. We fill this gap by studying a nonlinear weighted model of cascade failure with overloaded edges over synthetic and real weighted networks. The cascading failure model is constructed for the first time according to the overload coefficient, capacity parameter, weight coefficient, and distribution coefficient. Then through theoretical analysis, the conditions for stopping failure cascades are obtained, and the analysis shows the superiority of the constructed model. Finally, the cascading invulnerability is simulated in several typical network models and the US power grid. The results show that the model is a feasible and reasonable change of weight parameters, capacity coefficient, distribution coefficient, and overload coefficient can significantly improve the destructiveness of complex networks against cascade failure. Our methodology provides an efficacious reference for the control and prevention of cascading failures in many real networks.
Comparative structural analyses and nucleotide-binding characterization of the four KH domains of FUBP1
Authors: Ni Xiaomin , Knapp Stefan , Chaikuad Apirat 
Published: August 2020

AbstractThe FUBP1-FUSE complex is an essential component of a transcription molecular machinery that is necessary for tight regulation of expression of many key genes including c-Myc and p21. FUBP1 utilizes its four articulated KH modules, which function cooperatively, for FUSE nucleotide binding. To understand molecular mechanisms fundamental to the intermolecular interaction, we present a set of crystal structures, as well ssDNA-binding characterization of FUBP1 KH domains. All KH1-4 motifs were highly topologically conserved, and were able to interact with FUSE individually and independently. Nevertheless, differences in nucleotide binding properties among the four KH domains were evident, including higher nucleotide-binding potency for KH3 as well as diverse nucleotide sequence preferences. Variations in amino acid compositions at one side of the binding cleft responsible for nucleobase resulted in diverse shapes and electrostatic charge interaction, which might feasibly be a contributing factor for different nucleotide-binding propensities among KH1-4. Nonetheless, conservation of structure and nucleotide-binding property in all four KH motifs is essential for the cooperativity of multi KH modules present in FUBP1 towards nanomolar affinity for FUSE interaction. Comprehensive structural comparison and ssDNA binding characteristics of all four KH domains presented here provide molecular insights at a fundamental level that might be beneficial for elucidating the mechanisms of the FUBP1-FUSE interaction.
Whole genome and in-silico analyses of G1P[8] rotavirus strains from pre- and post-vaccination periods in Rwanda
Authors: Rasebotsa Sebotsana , Mwangi Peter N. , Mogotsi Milton T. , Sabiu Saheed , Magagula Nonkululeko B. , Rakau Kebareng , Uwimana Jeannine , Mutesa Leon , Muganga Narcisse , Murenzi Didier , Tuyisenge Lisine , Jaimes Jose , Esona Mathew D. , Bowen Michael D. , Mphahlele M. Jeffrey , Seheri Mapaseka L. , Mwenda Jason M. , Nyaga Martin M. 
Published: August 2020

AbstractRwanda was the first low-income African country to introduce RotaTeq vaccine into its Expanded Programme on Immunization in May 2012. To gain insights into the overall genetic make-up and evolution of Rwandan G1P[8] strains pre- and post-vaccine introduction, rotavirus positive fecal samples collected between 2011 and 2016 from children under the age of 5 years as part of ongoing surveillance were genotyped with conventional RT-PCR based methods and whole genome sequenced using the Illumina MiSeq platform. From a pool of samples sequenced (n = 158), 36 were identified as G1P[8] strains (10 pre-vaccine and 26 post-vaccine), of which 35 exhibited a typical Wa-like genome constellation. However, one post vaccine strain, RVA/Human-wt/RWA/UFS-NGS:MRC-DPRU442/2012/G1P[8], exhibited a RotaTeq vaccine strain constellation of G1-P[8]-I2-R2-C2-M2-A3-N2-T6-E2-H3, with most of the gene segments having a close relationship with a vaccine derived reassortant strain, previously reported in USA in 2010 and Australia in 2012. The study strains segregated into two lineages, each containing a paraphyletic pre- and post-vaccine introduction sub-lineages. In addition, the study strains demonstrated close relationship amongst each other when compared with globally selected group A rotavirus (RVA) G1P[8] reference strains. For VP7 neutralization epitopes, amino acid substitutions observed at positions T91A/V, S195D and M217T in relation to the RotaTeq vaccine were radical in nature and resulted in a change in polarity from a polar to non-polar molecule, while for the VP4, amino acid differences at position D195G was radical in nature and resulted in a change in polarity from a polar to non-polar molecule. The polarity change at position T91A/V of the neutralizing antigens might play a role in generating vaccine-escape mutants, while substitutions at positions S195D and M217T may be due to natural fluctuation of the RVA. Surveillance of RVA at whole genome level will enhance further assessment of vaccine impact on circulating strains, the frequency of reassortment events under natural conditions and epidemiological fitness generated by such events.
Reactive saccade adaptation boosts orienting of visuospatial attention
Authors: Nicolas Judith , Bidet-Caulet Aurélie , Pélisson Denis 
Published: August 2020

AbstractAttention and saccadic eye movements are critical components of visual perception. Recent studies proposed the hypothesis of a tight coupling between saccadic adaptation (SA) and attention: SA increases the processing speed of unpredictable stimuli, while increased attentional load boosts SA. Moreover, their cortical substrates partially overlap. Here, we investigated for the first time whether this coupling in the reactive/exogenous modality is specific to the orienting system of attention. We studied the effect of adaptation of reactive saccades (RS), elicited by the double-step paradigm, on exogenous orienting, measured using a Posner-like detection paradigm. In 18 healthy subjects, the attentional benefit—the difference in reaction time to targets preceded by informative versus uninformative cues—in a control exposure condition was subtracted from that of each adaptation exposure condition (backward and forward); then, this cue benefit difference was compared between the pre- and post-exposure phases. We found that, the attentional benefit significantly increased for cued-targets presented in the left hemifield after backward adaptation and for cued-targets presented in the right hemifield after forward adaptation. These findings provide strong evidence in humans for a coupling between RS adaptation and attention, possibly through the activation of a common neuronal pool.
Excitation of surface plasma waves and fast electron generation in relativistic laser–plasma interaction
Authors: Raynaud M. , Héron A. , Adam J.-C. 
Published: August 2020

AbstractThe excitation of surface plasma waves (SPW) by an intense short laser pulse is a useful tool to enhance the laser absorption and the electron heating in the target. In this work, the influence of the transverse laser profile and the pulse duration used to excited SPW is investigated from Fluid and 2D Particle-in-Cell simulations. We show the existence of a lobe of surface plasma wave modes. Our results highlight surface plasma waves excitation mechanism and define the laser parameters to optimise the SPW excitation and the kinetic energy of the associated electron trapped in the wave. It opens the door to monitor the spectral mode distribution and temporal shape of the excited surface waves in the high relativistic regime. The most important result of the study is that—at least in 2D—the charge and the energy of the electron bunches depend essentially on the laser energy rather than on temporal or spatial shape of the laser pulse.
View-flipping effect reduction and reconstruction visualization enhancement for EPISM based holographic stereogram with optimized hogel size
Authors: Yan Xingpeng , Zhang Teng , Wang Chenqing , Liu Yunpeng , Wang Ziqiang , Wang Xi , Zhang Zhe , Lin Min , Jiang Xiaoyu 
Published: August 2020

AbstractTo reduce the view-flipping effect and enhance the viewing resolution, the modulation characteristics of the hogel based holographic stereogram is constructed and validated. The performance of the view-flipping effect is analyzed, and the results indicate that decreasing the size of hogel is beneficial to the reduction of the view flipping, however, which will result in significant diffraction effect which can degrade the reconstruction quality. Furthermore, a diffraction-limited imaging model of the hogel based holographic stereogram is established, where both the limited aperture of the hogel and the defocused aberration of the object point are introduced, and the effective resolvable size of the reconstructed image point is simulated. The theory shows that there is an optimal hogel size existed for the certain depth of scene. Both the numerical and optical experiments are implemented, and the results are well agreed with the theoretical prediction, which demonstrates that the view-flipping reduction and reconstruction visualization enhancement for EPISM based holographic stereogram can be achieved when the proper size of hogel is utilized.