bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patients with IVF complicated by moderate-to-critical OHSS experience increased thrombosis,GDM and neonatal NICU admission but slightly shorter gestation compared with matched IVF counterparts: A retrospective Chinese cohort study
Authors: Hu Linli , Xie Rui , Wang Mengying , Sun Yingpu 
Published: November 2020

Abstract
Background: Ovarian hyperstimulation syndrome (OHSS) is a common disease during controlled ovarian hyperstimulation treatment. However, the obstetric and neonatal outcomes of this group of patients are unknown. The aim of this study was to explore the effects of late moderate-to-critical OHSS on obstetric and neonatal outcomes.Methods: This prospective observational study included 17,537 patients who underwent IVF/ICSI-fresh embryo transfer (ET) between June 2012 and July 2016 and met the inclusion criteria, including 7,064 eligible patients diagnosed with clinical pregnancy. Ultimately, 6,356 patients were allocated to the control group, and 385 patients who were hospitalized and treated at the center for late moderate-to-critical OHSS were allocated to the OHSS group. Then, propensity score matching analysis was performed, matching nine maternal baseline covariates and the number of multiple gestations; 385 patients with late moderate-to-critical OHSS were compared with a matched control group of 1,540 patients. The primary outcomes were the live birth rate, preterm delivery rate, miscarriage rate, gestational age at birth (weeks), obstetric complications and neonatal complications.Results: The duration of gestation in the matched control group was significantly higher than that in the OHSS group. The live birth delivery rate did not significantly differ between the OHSS and matched control groups. The incidence rates of the obstetric complications venous thrombosis (VT) and gestational diabetes mellitus (GDM), neonatal complications and the number of neonates admitted to the NICU were significantly higher in the OHSS group than in the matched control group.Conclusions: Pregnant women undergoing IVF with fresh ET whose course is complicated by late moderate-to-critical OHSS appear to experience shortened gestation and increased obstetrical and neonatal complications compared with matched controls whose course is not complicated by OHSS. However, the live birth rate, average neonatal weight, and incidence rates of premature delivery, miscarriage, early abortion, hypertensive disorder of pregnancy (HDP), placenta previa (PP), intrahepatic cholestasis of pregnancy (ICP), and low neonatal birth weight (LBW) did not differ significantly between the two groups.
Novel thermoresponsive polymer outperforming Pluronic® F127
Authors: Constantinou Anna , Nele Valeria , Doutch James , Moiseev Roman , Khutoryanskiy Vitaliy , Stevens Molly , Georgiou Theoni 
Published: November 2020

Abstract
Thermoresponsive polymers featuring the appropriate combination of structural characteristics, i.e. architecture, composition, and molar mass (MM), can form physically crosslinked networks in a solvent upon changes in temperature. This fascinating class of polymers finds utility in various sectors such as formulation science and tissue engineering. Here, we report a novel thermoresponsive triblock terpolymer which out-performs the most commonly used and commercially available thermoresponsive polymer, Poloxamer P407 (also known as Pluronic® F127) in terms of gelation concentration. Specifically, the in-house synthesised polymer forms gels at lower concentrations that is an advantage in biomedical applications. To elucidate the differences in their macroscale gelling behaviour, we investigate their micellization via differential scanning calorimetry, and their nanoscale self-assembly behaviour in detail by means of small-angle neutron scattering by simultaneously recording their rheological properties (Rheo-SANS). Two different gelation mechanisms for the two polymers are revealed and proposed. Ex vivo gelation study upon intracameral injections demonstrated excellent potential for its application to improve drug residence in the eye.
A Meta-Analysis of the Epidemiology of Giant Cell Arteritis Across Time and Space
Authors: Li Katherine , Semenov Daniel , Turk Matthew , Pope Janet 
Published: November 2020

Abstract
Introduction: Giant cell arteritis (GCA) is the most common large vessel vasculitis in those over age 50 years. This meta-analysis examined the geographical and temporal distribution of the incidence, prevalence and mortality of GCA.Methods: A systematic review was conducted using EMBASE, Scopus and PubMed from their inceptions until 2019. Studies were included if they reported at least 50 or more GCA patients and defined the location and time frame. Articles on mortality were included and standardized mortality ratio (SMR) was extracted where possible. Mean pooled prevalence, incidence, and SMR were calculated using a random effects model. Linear regression was used to explore correlations between latitude and incidence, prevalence, and mortality.Results: Of the 3569 citations identified, 107 were included. The pooled incidence of GCA was 10.00 [9.22, 10.78] cases per 100,000 people over 50 years old. This incidence was highest in Scandinavia 21.57 [18.90, 24.23], followed by North and South America 10.89 [8.78, 13.00], Europe 7.26 [6.05, 8.47], and Oceania 7.85 [-1.48, 17.19]. Pooled prevalence was 51.74 [42.04, 61.43] cases per 100,000 people over age 50. Annual mortality was 20.44 [17.84, 23.03] deaths/1000. Mortality generally decreased over the years of publication (p=0.0008). Latitude correlated significantly with incidence (p=0.0011), but not with prevalence, or mortality.Conclusions: GCA incidence varies nearly 3-fold between regions and is highest in Scandinavia but not significantly. Mortality may be improving over time.
Tripartite Motif-Containing 3 (TRIM3) Enhances ER Signaling and Confers Tamoxifen Resistance in Breast Cancer
Authors: Ye Run-yi , Kuang Xia-ying , Zeng Hui-juan , Shao Nan , lin ying , liu pian , wang shenming 
Published: November 2020

Abstract
Background: Tamoxifen resistance remains a tricky clinical problem in estrogen receptor (ER)-positive breast cancer. SUMOylation of ERα enhances ERα-induced transcription activity. TRIM proteins are a new class of SUMO E3 ligases, regulating SUMOylation of proteins. However, the precise molecular mechanism and function of TRIM3 in SUMOylation and response to tamoxifen remains unclear.Methods: The expression of TRIM3 was assessed in 48 cases with breast cancer which received tamoxifen therapy. The protein and RNA levels of TRIM3 in 12 ER+ breast cancer cell lines were assessed by Western blotting and qRT-PCR assays. Effects of TRIM3 in regulating tamoxifen resistance were evaluated both in vitro and in vivo via Cell viability, Colony formation, Anchorage-independent growth ability assays and Tumor xenografts. Molecular mechanism of TRIM3 in regulating SUMOylation of ERα was analyzed by Western blotting, Immunoprecipitation, Far Western blotting and Luciferase activity assays.Results: We reported that TRIM3 was dramatically overexpressed in breast cancer, which correlated with tamoxifen resistance., Furthermore,TRIM3 overexpression significantly correlated with poor survival of ER+ breast cancer treated with tamoxifen. TRIM3 overexpression conferred cell survival and tumorigenesis, whereas knocking downTRIM3 reduced these capabilities. Moreover, TRIM3, as a UBC9 binding protein, promoted SUMO modification of ESR1 and activated ER pathway. Silencing UBC9 abolished the function of TRIM3 in regulating tamoxifen resistance.Conclusions: These results suggest a novel biomarker for breast cancer therapy, indicating that inhibiting TRIM3 combined with tamoxifen may provide a potential treatment for breast cancer.
WITHDRAWN: Synthesis and Characterization of SrHfO3 Co-doped with Gd2O3 and Yb2O3 (SHGY)
Authors: Kandi Hemalatha , Koona Prof.Ramji , Raju G.M.J 
Published: November 2020

Abstract
A new material SHGY is obtained after synthesizing SrHfO3 co-doped with rare earth oxides (5 mol%Gd2O3 and 5 mol% Yb2O3). This process is carried out by a mechanical solid-state synthesis method. The synthesized powder is annealed at 1450ºC keeping rate of increase of 3ºC/min for 2hours. The phase and structural information of synthesized powder is characterized by X-Ray Diffraction (XRD) technique. The powder sample is sintered by Spark Plasma Sintering (SPS) at 1700ºC for 15 minutes to form into a pellet. The microstructure analysis is done by using Field Emission Scanning Electron Microscopy (FESEM) followed by composition analysis by Energy Dispersive Spectroscopy (EDS). The phase purity is examined by Thermal Gravimetric Analysis and Differential Thermal Analysis (TG-DTA) and studies conclude that weight loss is < 3% and there are no changes in phase transformation. Finally, Thermal Expansion Coefficient (CTE) is studied by a Dilatometer. Results stated there is an increase in the CTE in the range of 8.82-13.0 × 10− 6K− 1 calculated temperature range 100–1000ºC. This result of CTE showed there is a 19.2% (10.5 × 10− 6K− 1(200–1100ºC)) increase compared to the YSZ. This aids in the reduction of thermal mismatches between the substrate and topcoat in Thermal barrier applications (TBC).
Metagenomics Analysis Reveals An Extraordinary Bacterial Diversity in Anisakids (Nematoda: Anisakidae) L3 Larvae
Authors: Arcos Susana C. , Lira Felipe , Robertson Lee , González María Rosa , Carballeda-Sangiao Noelia , Sanchez-Alonso Isabel , Zamorano Laura , Careche Mercedes , Jiménez-Ruíz Yolanda , Ramos Ricardo , Llorens Carlos , González-Muñoz Miguel , Oliver Antonio , Martínez José Luis , Navas Alfonso 
Published: November 2020

Abstract
Background: L3 larvae of anisakid nematodes are an important problem for the fisheries industry and pose a potential risk for human health by acting as agents causing allergies and as potential vectors of pathogens microrganisms. In spite of the close bacteria-nematode relationship very little is known of the Anisakids microbiote. Fresh fish could be contaminated by bacteria vectored in the cuticle or in the intestine of Anisakids when the L3 larvae migrate through the muscles. As a consequence the bacterial inoculum will be spread, affecting the quality of the fish, and possible clinical effects cannot be discardedResults: A total of 2,689,113 16S rRNA gene sequences from a total of 113 L3 individuals obtained from fish captured along the FAO 27 area were studied. Bacteria were taxonomically characterized through 1803 representative OTUsequences. Fourteen Phyla, 31 Classes, 52 Orders, 129 Families and 187 genera were unambiguously identified. We have found as part of microbiome an average of 123 OTUs per L3 individual. Diversity Indices (Shannon and Simpson indices) indicate an extraordinary diversity of bacteria at a OTU level. There are clusters of Anisakids individuals (samples) defined by the associated bacteria which however are not significantly related with fish hosts or anisakid taxa. This suggests that association or relationship among bacteria in Anisakids, exists without the influence of fishes or nematodes. The lack of relationships with hosts of Anisakids taxa has to be necessarily expresssed by the association among bacterial OTUs or other taxonomical levels which are ranging from OTUs to phylum level. Conclusions: There are significant biological structural associations of microbiota in anisakid nematodes which manifest in clusters of bacteria ranging from plyllum to genus level which could also be anindicator of degree of fish contamination or from the geographic zone of fish capture. Actinobacteria, Aquificae, Firmicutes, and Proteobacteria are the phyla whose abundance value discriminate for difining such structures.
Overlap Time is An Independent Risk Factor of Radiation Pneumonitis for Patients Treated With Simultaneous EGFR-TKI and Thoracic Radiotherapy
Authors: Jia Wenxiao , Gao Qianqian , Wang Min , Li Ji , Jing Wang , Yu Jinming , Zhu Hui 
Published: November 2020

Abstract
Background: The exact rate and relevant risk factors of radiation pneumonitis (RP) for non-small-cell cancer (NSCLC) patients treated with the combination of epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) and thoracic radiotherapy have not been reported. Thus, this study aimed to investigate the rate and risk factors of RP for EGFR-positive NSCLC patients simultaneously treated with first-generation EGFR-TKI and TRT.Patients and Methods: We retrospectively evaluated NSCLC patients simultaneously treated with first-generation EGFR-TKI and thoracic radiotherapy between January 2012 and December 2019 at Shandong Cancer Hospital and Institute, Shandong, China. RP was diagnosed via computed tomography and was classified according to the Common Terminology Criteria for Adverse Events v5.0. The risk factors of RP were identified using uni- and multivariate analyses.Results: Of the 67 patients included, 44.78% (30/67) developed grade ≥2 RP. Grade ≥2 RP occurred within a median of 3.48 (range, 1.07-13.6) months. The EGFR-TKI icotinib, ipsilateral lung V30 >34%, and overlap time of >20 days between EGFR-TKI and thoracic radiotherapy were identified to be independent predictive factors of grade ≥2 RP.Conclusions: Grade ≥2 RP is highly frequent in NSCLC patients simultaneous treated with first-generation EGFR-TKI and thoracic radiotherapy. Icotinib, ipsilateral lung V30 <34%, and shorter overlap time of <20 days for EGFR-TKI and thoracic radiotherapy will help lower the risk of RP in these patients. The addition of thoracic radiotherapy should be cautious, and the treatment strategies can be optimized to reduce the rate of RP for patients treat with simultaneous EGFR-TKI and thoracic radiotherapy.
Origin, Behaviour, and Genetics of Reproductive Workers in an Invasive Ant
Authors: Lenancker Pauline , Feldhaar Heike , Holzinger Anja , Greenfield Melinda , Strain Angela , Yeeles Peter , Hoffmann Benjamin D , Tay Wee Tek , Lach Lori 
Published: November 2020

Abstract
Background: Worker reproduction has an important influence on the social cohesion and efficiency of social insect colonies, but its role in the success of invasive ants has been neglected. We used observations of 233 captive colonies, laboratory experiments, and genetic analyses to investigate the conditions for worker reproduction in the invasive Anoplolepis gracilipes (yellow crazy ant) and its potential cost on interspecific defence. We determined how common worker production of males is and whether it is triggered by queen absence; whether physogastric workers with enlarged abdomens are more likely to be reproductive, how normal workers and physogastric workers compare in their contributions to colony labour and defence; and whether worker-produced males and males that could have been queen- or worker-produced differ in their size and heterozygosity. Results: Sixty-six of our 233 captive colonies produced males, and in 25 of these, some males could only have been produced by workers. Colonies with more workers were more likely to produce males, especially for queenless colonies. The average number of days between the first appearance of eggs and adult males in our colonies was 54.1± 10.2 (mean ± SD, n=20). In our laboratory experiment, queen removal triggered an increase in the proportion of physogastric workers. Physogastric workers were more likely to have yolky oocytes (37-54.9%) than normal workers (2-25.6%), which is an indicator of fertile or trophic egg production. Physogastric workers were less aggressive during interspecific aggression tests and foraged less than normal workers. The head width and wing length of worker-produced males were on average 4.0% and 4.3% greater respectively than those of males of undetermined source. Our microsatellite DNA analyses indicate that 4.9% of worker-produced males and 21.1% of males of undetermined source were heterozygous, which suggests the presence of diploid males and/or genetic mosaics in A. gracilipes. Conclusions: Our experimental work provides crucial information on worker reproduction in A. gracilipes and its potential cost to colony defence. The ability of A. gracilipes workers to produce males in the absence of queens may also contribute to its success as an invasive species if intranidal mating can take place between virgin queens and worker-produced males.
Quantitative proteomics analysis of Mycoplasma pneumoniae identifies potential macrolide resistance determinants
Authors: li shaoli , xue guanhua , zhao hanqing , feng yanling , yan chao , cui jinghua , xie xianghui , yuan jing 
Published: November 2020

Abstract
Mycoplasma pneumoniae is one of the leading causes of community-acquired pneumonia in children and adolescents. Because of the wide application of macrolides in clinical treatment, macrolide-resistant M. pneumoniae strains have become increasingly common worldwide. However, the molecular mechanisms underlying drug resistance in M. pneumoniae are poorly understood. In the present work, we analyzed the whole proteomes of macrolide-sensitive and macrolide-resistant strains of M. pneumoniae using a tandem mass tag-labeling quantitative proteomic technique, Data are available via ProteomeXchange with identifier PXD022220. In total, 165 differentially expressed proteins were identified, of which 80 were upregulated and 85 were downregulated in the drug-resistant strain compared with the sensitive strain. Functional analysis revealed that these proteins were predominantly involved in protein and peptide biosynthesis processes, the ribosome, and transmembrane transporter activity, which implicates them in the mechanism(s) of resistance of M. pneumoniae to macrolides. Our results provide new insights into drug resistance in M. pneumoniae and identify potential targets for further studies on resistance mechanisms in this bacterium.
Reporting Quality of Clinical Practice Guidelines Regarding Gout and Hyperuricemia According to the Right Checklist
Authors: Wang Can , Luo Xufei , Li Maichao , Cui Lingling , Li Xinde , Han Lin , Wang Xuefeng , Ren Wei , He Yuwei , Sun Wenyan , Li Changgui , Chen Yaolong , Liu Zhen 
Published: November 2020

Abstract
Objectives The Reporting Items for Practice Guidelines in Healthcare (RIGHT) checklist was used to assess the reporting quality of 2009–2019 Clinical Practice Guidelines (CPGs) regarding gout and hyperuricemia, aimed to improve the reporting quality of future guidelines.Methods We searched PubMed, the Chinese Biomedical Literature database, the Wan Fang Database, and the China National Knowledge Infrastructure from January 2009 to June 2019 for relevant guidelines. We also searched the websites of guideline development organizations (the Guidelines International Network, the National Institute for Health and Clinical Excellence, the American College of Rheumatology, and the European League Against Rheumatism) (EULAR). Furthermore, supplementary guidelines reported in included articles were systematically searched, as well as Medlive and Google Scholar. Results Seventeen guidelines were included, of which one was in Chinese and 16 were in English. The mean reporting rate of the 35 items specified was 14.9 (42.5%), only five CPGs (29.4%) had a reporting rate >50%. Of the 35 items, three were very frequently reported. The reporting proportion of the seven domains (Basic information, Background, Evidence, Recommendations, Review and quality assurance, Funding and declaration and management of interests, and Other information) were 64.7%, 36.8%, 50.6% 50.6%, 42.9%, 8.82%, 33.8%, and 31.4%, respectively.Conclusion The reporting quality of the present guidelines for gout and hyperuricemia is relatively poor. We suggest that the RIGHT reporting checklist should be used by CPG developers to ensure higher reporting quality of future guidelines.
Reducing the metabolic energy of walking and running using an unpowered hip exoskeleton
Authors: Zhou Tiancheng , Xiong Caihua , Zhang Juanjuan , Hu Di , Chen Wenbin , Huang Xiaolin 
Published: November 2020

Abstract
Background: Walking and running are the most common means of locomotion in human daily life. People have made advances in developing separate exoskeletons to reduce metabolic rate of walking or running. However, the combined requirements of overcoming fundamental biomechanical differences between the two gaits and minimizing the metabolic penalty of exoskeleton mass make it challenging to develop an exoskeleton that can reduce the metabolic energy for both gaits. Here we show that the metabolic energy of both walking and running can be reduced by regulating the metabolic energy of hip flexion during the common energy consumption period of the two gaits using an unpowered hip exoskeleton. Methods: We analyzed metabolic rates, muscle activities and spatio-temporal parameters from 9 healthy subjects (mean s.t.d; 24.9 ± 3.7 years, 66.9 ± 8.7 kg, 1.76 ± 0.05 m) walking on a treadmill at the speed of 1.5 m×s -1 and running at speed of 2.5 m×s -1 with different spring stiffnesses. After obtaining the optimal spring stiffness, we recruited the participants to walk and run with the optimal stiffness spring at different speeds to demonstrate the generality of the proposed approach. Results: We found that the optimal exoskeleton spring stiffnesses for walking and running were 140 N×m Rad -1 and 210 N×m Rad -1 respectively, corresponding to 8.2% ± 1.5% (mean ± s.e.m, two-sided paired t-test: p < 0.01) and 9.1% ± 1.3% ( p < 0.01) metabolic reductions compared to walking/running without exoskeleton. The metabolic energy within tested speed range can be reduced with the assistance except for low speed walking (1.0 m s -1 ). Participants showed different changes in muscle activities with the assistance of proposed exoskeleton. Conclusions: This paper first demonstrated that metabolic cost of walking and running can be reduced using an unpowered hip exoskeleton to regulate metabolic energy of hip flexion. The design method based on analyzing the common energy consumption characteristics between gaits may inspire future exoskeletons that assist multiple gaits. The results of different changes in muscle activities provided a new insight of human response to the same assistive principle in different gaits (walking and running).
Hydrodynamics-driven uncertainty study in simulating terrestrial carbon cycle with a coupled water-carbon cycle model
Authors: Sun Guodong , Mu Mu 
Published: November 2020

Abstract
In this study, an uncertainty degree of net primary production (NPP) simulation or prediction is estimated due to uncertainty of hydrodynamic processes. The Lund-Potsdam-Jena Wetland Hydrology and Methane Dynamic Global Vegetation Model (LPJ-WHyMe DGVM), which could describe the interaction between terrestrial carbon cycle and hydrological cycle, is applied to evaluate the above issue. Four regions with different climate backgrounds in China are chosen to explore different behaviors about the influence of the simulated NPP to hydrological physical processes on. It is found that the error of NPP modeling is 198.5 g C m− 2 yr− 1 (48.6%) in the arid and semiarid region of northern China due to the uncertainties in the hydrological processes, which is greater than those in other three regions. The errors of NPP modeling are 88.4 g C m− 2 yr− 1 (19.1%) in northeastern China, 117.0 g C m− 2 yr− 1 (21.1%) in southern China, and 64.4 g C m− 2 yr− 1 (13.2%) in the semi-humid region of northern China. Further numerical experiments reveal that the simulation ability of the NPP could be enhanced by reducing all hydrological parameter errors. Our results demonstrate the importance of hydrological processes to the simulation and prediction of the NPP.