bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementing parasite genotyping into national surveillance frameworks: feedback from control programmes and researchers in the Asia–Pacific region
Authors: Noviyanti Rintis , Miotto Olivo , Barry Alyssa , Marfurt Jutta , Siegel Sasha , Thuy-Nhien Nguyen , Quang Huynh Hong , Anggraeni Nancy Dian , Laihad Ferdinand , Liu Yaobao , Sumiwi Maria Endang , Trimarsanto Hidayat , Coutrier Farah , Fadila Nadia , Ghanchi Najia , Johora Fatema Tuj , Puspitasari Agatha Mia , Tavul Livingstone , Trianty Leily , Utami Retno Ayu Setya , Wang Duoquan , Wangchuck Kesang , Price Ric N. , Auburn Sarah 
Published: July 2020

AbstractThe Asia–Pacific region faces formidable challenges in achieving malaria elimination by the proposed target in 2030. Molecular surveillance of Plasmodium parasites can provide important information on malaria transmission and adaptation, which can inform national malaria control programmes (NMCPs) in decision-making processes. In November 2019 a parasite genotyping workshop was held in Jakarta, Indonesia, to review molecular approaches for parasite surveillance and explore ways in which these tools can be integrated into public health systems and inform policy. The meeting was attended by 70 participants from 8 malaria-endemic countries and partners of the Asia Pacific Malaria Elimination Network. The participants acknowledged the utility of multiple use cases for parasite genotyping including: quantifying the prevalence of drug resistant parasites, predicting risks of treatment failure, identifying major routes and reservoirs of infection, monitoring imported malaria and its contribution to local transmission, characterizing the origins and dynamics of malaria outbreaks, and estimating the frequency of Plasmodium vivax relapses. However, the priority of each use case varies with different endemic settings. Although a one-size-fits-all approach to molecular surveillance is unlikely to be applicable across the Asia–Pacific region, consensus on the spectrum of added-value activities will help support data sharing across national boundaries. Knowledge exchange is needed to establish local expertise in different laboratory-based methodologies and bioinformatics processes. Collaborative research involving local and international teams will help maximize the impact of analytical outputs on the operational needs of NMCPs. Research is also needed to explore the cost-effectiveness of genetic epidemiology for different use cases to help to leverage funding for wide-scale implementation. Engagement between NMCPs and local researchers will be critical throughout this process.
CD36-mediated uptake of myelin debris by macrophages and microglia reduces neuroinflammation
Authors: Grajchen Elien , Wouters Elien , van de Haterd Britt , Haidar Mansour , Hardonnière Kévin , Dierckx Tess , Van Broeckhoven Jana , Erens Celine , Hendrix Sven , Kerdine-Römer Saadia , Hendriks Jerome J. A. , Bogie Jeroen F. J. 
Published: July 2020

Abstract
Background
The presence of foamy macrophages and microglia containing intracellular myelin remnants is a pathological hallmark of neurodegenerative disorders such as multiple sclerosis (MS). Despite the importance of myelin internalization in affecting both central nervous system repair and neuroinflammation, the receptors involved in myelin clearance and their impact on the phagocyte phenotype and lesion progression remain to be clarified.

Methods
Flow cytometry, quantitative PCR, and immunohistochemistry were used to define the mRNA and protein abundance of CD36 in myelin-containing phagocytes. The impact of CD36 and nuclear factor erythroid 2–related factor 2 (NRF2) on the phagocytic and inflammatory features of macrophages and microglia was assessed using a pharmacological CD36 inhibitor (sulfo-N-succinimidyl oleate) and Nrf2−/− bone marrow-derived macrophages. Finally, the experimental autoimmune encephalomyelitis (EAE) model was used to establish the impact of CD36 inhibition on neuroinflammation and myelin phagocytosis in vivo.

Results
Here, we show that the fatty acid translocase CD36 is required for the uptake of myelin debris by macrophages and microglia, and that myelin internalization increased CD36 expression through NRF2. Pharmacological inhibition of CD36 promoted the inflammatory properties of myelin-containing macrophages and microglia in vitro, which was paralleled by a reduced activity of the anti-inflammatory lipid-sensing liver X receptors and peroxisome proliferator-activated receptors. By using the EAE model, we provide evidence that CD36 is essential for myelin debris clearance in vivo. Importantly, CD36 inhibition markedly increased the neuroinflammatory burden and disease severity in the EAE model.

Conclusion
Altogether, we show for the first time that CD36 is crucial for clearing myelin debris and suppressing neuroinflammation in demyelinating disorders such as MS.
Passive-elastic knee-ankle exoskeleton reduces the metabolic cost of walking
Authors: Etenzi Ettore , Borzuola Riccardo , Grabowski Alena M. 
Published: July 2020

Abstract
Background
Previous studies have shown that passive-elastic exoskeletons with springs in parallel with the ankle can reduce the metabolic cost of walking. We developed and tested the use of an unpowered passive-elastic exoskeleton for walking that stores elastic energy in a spring from knee extension at the end of the leg swing phase, and then releases this energy to assist ankle plantarflexion at the end of the stance phase prior to toe-off. The exoskeleton uses a system of ratchets and pawls to store and return elastic energy through compression and release of metal springs that act in parallel with the knee and ankle, respectively. We hypothesized that, due to the assistance provided by the exoskeleton, net metabolic power would be reduced compared to walking without using an exoskeleton.

Methods
We compared the net metabolic power required to walk when the exoskeleton only acts at the knee to resist extension at the end of the leg swing phase, to that required to walk when the stored elastic energy from knee extension is released to assist ankle plantarflexion at the end of the stance phase prior to toe-off. Eight (4 M, 4F) subjects walked at 1.25 m/s on a force-measuring treadmill with and without using the exoskeleton while we measured their metabolic rates, ground reaction forces, and center of pressure.

Results
We found that when subjects used the exoskeleton with energy stored from knee extension and released for ankle plantarflexion, average net metabolic power was 11% lower than when subjects walked while wearing the exoskeleton with the springs disengaged (p = 0.007), but was 23% higher compared to walking without the exoskeleton (p < 0.0001).

Conclusion
The use of a novel passive-elastic exoskeleton that stores and returns energy in parallel with the knee and ankle, respectively, has the potential to improve the metabolic cost of walking. Future studies are needed to optimize the design and elucidate the underlying biomechanical and physiological effects of using an exoskeleton that acts in parallel with the knee and ankle. Moreover, addressing and improving the exoskeletal design by reducing and closely aligning the mass of the exoskeleton could further improve the metabolic cost of walking.
Oxygen: viral friend or foe?
Authors: Gan Esther Shuyi , Ooi Eng Eong 
Published: July 2020

AbstractThe oxygen levels organ and tissue microenvironments vary depending on the distance of their vasculature from the left ventricle of the heart. For instance, the oxygen levels of lymph nodes and the spleen are significantly lower than that in atmospheric air. Cellular detection of oxygen and their response to low oxygen levels can exert a significant impact on virus infection. Generally, viruses that naturally infect well-oxygenated organs are less able to infect cells under hypoxic conditions. Conversely, viruses that infect organs under lower oxygen tensions thrive under hypoxic conditions. This suggests that in vitro experiments performed exclusively under atmospheric conditions ignores oxygen-induced modifications in both host and viral responses. Here, we review the mechanisms of how cells adapt to low oxygen tensions and its impact on viral infections. With growing evidence supporting the role of oxygen microenvironments in viral infections, this review highlights the importance of factoring oxygen concentrations into in vitro assay conditions. Bridging the gap between in vitro and in vivo oxygen tensions would allow for more physiologically representative insights into viral pathogenesis.
Morphologic evaluation of root resorption after miniscrew assisted en mass retraction in adult bialveolar protrusion patients
Authors: Chen Yu , Liu Dongxu 
Published: July 2020

Abstract
Background
Bialveolar protrusion is one of the most common chief complaints from the Asian orthodontic patients. Typical orthodontic treatment includes extraction of the bimaxillary premolars and en mass retraction of anterior tooth with maximum anchorage by placing miniscrews. However, excessive pursuit of profile improvement by retraction and intrusion of anterior teeth may result in root resorption, alveolar bone loss, even dehiscence. Thus this retrospective, analytical study was to evaluate the root resorption of anterior teeth after miniscrew assisted en mass retraction in adult bialveolar protrusion patients.

Materials and methods
Thirty six adult patients with bimaxillary protrusion had four first premolars extracted, and then miniscrews were placed to provide anchorage. CBCT scans were performed before (T1) and posttreatment (T2). A new improvement project introduced for 3D CBCT registration assessment of root morphology. The paired t-test was used to compare changes from T1 to T2. The relationship between the root resorption and the movement of anterior teeth were assessed by Pearson correlation coefficient analysis.

Results
The significant differences were only found in apical third of root and the largest resorption in apical third of the root is always noted in the palatal and distal sectors. Significant correlations were observed in the loss of root in distal and palatal sectors, the root length and volume decrease with the amount of anterior teeth retraction and intrusion.

Conclusion
The new 3D registration assessment of root morphology will be helpful for the clinicians. Pursuit of large retraction and intrusion leads to obvious anterior teeth root resorption.
Plasma lipid profiling for the prognosis of 90-day mortality, in-hospital mortality, ICU admission, and severity in bacterial community-acquired pneumonia (CAP)
Authors: Banoei Mohammad M. , Vogel Hans J. , Weljie Aalim M. , Yende Sachin , Angus Derek C. , Winston Brent W. 
Published: July 2020

Abstract
Introduction
Pneumonia is the most common cause of mortality from infectious diseases, the second leading cause of nosocomial infection, and the leading cause of mortality among hospitalized adults. To improve clinical management, metabolomics has been increasingly applied to find specific metabolic biopatterns (profiling) for the diagnosis and prognosis of various infectious diseases, including pneumonia.

Methods
One hundred fifty bacterial community-acquired pneumonia (CAP) patients whose plasma samples were drawn within the first 24 h of hospital admission were enrolled in this study and separated into two age- and sex-matched cohorts: non-survivors (died ≤ 90 days) and survivors (survived > 90 days). Three analytical tools, 1H-NMR spectroscopy, GC-MS, and targeted DI-MS/MS, were used to prognosticate non-survivors from survivors by means of metabolic profiles.

Results
We show that quantitative lipid profiling using DI-MS/MS can predict the 90-day mortality and in-hospital mortality among patients with bacterial CAP compared to 1H-NMR- and GC-MS-based metabolomics. This study showed that the decreased lysophosphatidylcholines and increased acylcarnitines are significantly associated with increased mortality in bacterial CAP. Additionally, we found that decreased lysophosphatidylcholines and phosphatidylcholines (> 36 carbons) and increased acylcarnitines may be used to predict the prognosis of in-hospital mortality for bacterial CAP as well as the need for ICU admission and severity of bacterial CAP.

Discussion
This study demonstrates that lipid-based plasma metabolites can be used for the prognosis of 90-day mortality among patients with bacterial CAP. Moreover, lipid profiling can be utilized to identify patients with bacterial CAP who are at the highest risk of dying in hospital and who need ICU admission as well as the severity assessment of CAP.
Provision of ECPR during COVID-19: evidence, equity, and ethical dilemmas
Authors: Worku Elliott , Gill Denzil , Brodie Daniel , Lorusso Roberto , Combes Alain , Shekar Kiran 
Published: July 2020

AbstractThe use of extracorporeal cardiopulmonary resuscitation (ECPR) to restore circulation during cardiac arrest is a time-critical, resource-intensive intervention of unproven efficacy. The current COVID-19 pandemic has brought additional complexity and significant barriers to the ongoing provision and implementation of ECPR services. The logistics of patient selection, expedient cannulation, healthcare worker safety, and post-resuscitation care must be weighed against the ethical considerations of providing an intervention of contentious benefit at a time when critical care resources are being overwhelmed by pandemic demand.
Characterisation of an inflammation-related epigenetic score and its association with cognitive ability
Authors: Stevenson Anna J. , McCartney Daniel L. , Hillary Robert F. , Campbell Archie , Morris Stewart W. , Bermingham Mairead L. , Walker Rosie M. , Evans Kathryn L. , Boutin Thibaud S. , Hayward Caroline , McRae Allan F. , McColl Barry W. , Spires-Jones Tara L. , McIntosh Andrew M. , Deary Ian J. , Marioni Riccardo E. 
Published: July 2020

AbstractBackgroundChronic systemic inflammation has been associated with incident dementia, but its association with age-related cognitive decline is less clear. The acute responses of many inflammatory biomarkers mean they may provide an unreliable picture of the chronicity of inflammation. Recently, a large-scale epigenome-wide association study identified DNA methylation correlates of C-reactive protein (CRP)—a widely used acute-phase inflammatory biomarker. DNA methylation is thought to be relatively stable in the short term, marking it as a potentially useful signature of exposure.MethodsWe utilise a DNA methylation-based score for CRP and investigate its trajectories with age, and associations with cognitive ability in comparison with serum CRP and a genetic CRP score in a longitudinal study of older adults (n= 889) and a large, cross-sectional cohort (n= 7028).ResultsWe identified no homogeneous trajectories of serum CRP with age across the cohorts, whereas the epigenetic CRP score was consistently found to increase with age (standardisedβ= 0.07 and 0.01) and to do so more rapidly in males compared to females. Additionally, the epigenetic CRP score had higher test-retest reliability compared to serum CRP, indicating its enhanced temporal stability. Higher serum CRP was not found to be associated with poorer cognitive ability (standardisedβ= − 0.08 and − 0.05); however, a consistent negative association was identified between cognitive ability and the epigenetic CRP score in both cohorts (standardisedβ= − 0.15 and − 0.08).ConclusionsAn epigenetic proxy of CRP may provide a more reliable signature of chronic inflammation, allowing for more accurate stratification of individuals, and thus clearer inference of associations with incident health outcomes.
Targeting PPARα in the rat valproic acid model of autism: focus on social motivational impairment and sex-related differences
Authors: Scheggi Simona , Guzzi Francesca , Braccagni Giulia , De Montis Maria Graziella , Parenti Marco , Gambarana Carla 
Published: July 2020

Abstract
Background
The social motivational theory of autism spectrum disorder (ASD) focuses on social anhedonia as key causal feature of the impaired peer relationships that characterize ASD patients. ASD prevalence is higher in boys, but increasing evidence suggests underdiagnosis and undertreatment in girls. We showed that stress-induced motivational anhedonia is relieved by repeated treatment with fenofibrate (FBR), a peroxisome proliferator-activated receptor α (PPARα) agonist. Here, we used the valproic acid (VPA) model of ASD in rats to examine male and female phenotypes and assess whether FBR administration from weaning to young adulthood relieved social impairments.

Methods
Male and female rats exposed to saline or VPA at gestational day 12.5 received standard or FBR-enriched diet from postnatal day 21 to 48–53, when behavioral tests and ex vivo neurochemical analyses were performed. Phosphorylation levels of DARPP-32 in response to social and nonsocial cues, as index of dopamine D1 receptor activation, levels of expression of PPARα, vesicular glutamatergic and GABAergic transporters, and postsynaptic density protein PSD-95 were analyzed by immunoblotting in selected brain regions.

Results
FBR administration relieved social impairment and perseverative behavior in VPA-exposed male and female rats, but it was only effective on female stereotypies. Dopamine D1 receptor signaling triggered by social interaction in the nucleus accumbens shell was blunted in VPA-exposed rats, and it was rescued by FBR treatment only in males. VPA-exposed rats of both sexes exhibited an increased ratio of striatal excitatory over inhibitory synaptic markers that was normalized by FBR treatment.

Limitations
This study did not directly address the extent of motivational deficit in VPA-exposed rats and whether FBR administration restored the likely decreased motivation to operate for social reward. Future studies using operant behavior protocols will address this relevant issue.

Conclusions
The results support the involvement of impaired motivational mechanisms in ASD-like social deficits and suggest the rationale for a possible pharmacological treatment. Moreover, the study highlights sex-related differences in the expression of ASD-like symptoms and their differential responses to FBR treatment.
Large-diameter trees dominate snag and surface biomass following reintroduced fire
Authors: Lutz James A. , Struckman Soren , Furniss Tucker J. , Cansler C. Alina , Germain Sara J. , Yocom Larissa L. , McAvoy Darren J. , Kolden Crystal A. , Smith Alistair M. S. , Swanson Mark E. , Larson Andrew J. 
Published: July 2020

AbstractThe reintroduction of fire to landscapes where it was once common is considered a priority to restore historical forest dynamics, including reducing tree density and decreasing levels of woody biomass on the forest floor. However, reintroducing fire causes tree mortality that can have unintended ecological outcomes related to woody biomass, with potential impacts to fuel accumulation, carbon sequestration, subsequent fire severity, and forest management. In this study, we examine the interplay between fire and carbon dynamics by asking how reintroduced fire impacts fuel accumulation, carbon sequestration, and subsequent fire severity potential. Beginning pre-fire, and continuing 6 years post-fire, we tracked all live, dead, and fallen trees ≥ 1 cm in diameter and mapped all pieces of deadwood (downed woody debris) originating from tree boles ≥ 10 cm diameter and ≥ 1 m in length in 25.6 ha of anAbies concolor/Pinus lambertianaforest in the central Sierra Nevada, California, USA. We also tracked surface fuels along 2240 m of planar transects pre-fire, immediately post-fire, and 6 years post-fire. Six years after moderate-severity fire, deadwood ≥ 10 cm diameter was 73 Mg ha−1, comprised of 32 Mg ha−1that persisted through fire and 41 Mg ha−1of newly fallen wood (compared to 72 Mg ha−1pre-fire). Woody surface fuel loading was spatially heterogeneous, with mass varying almost four orders of magnitude at the scale of 20 m × 20 m quadrats (minimum, 0.1 Mg ha−1; mean, 73 Mg ha−1; maximum, 497 Mg ha−1). Wood from large-diameter trees (≥ 60 cm diameter) comprised 57% of surface fuel in 2019, but was 75% of snag biomass, indicating high contributions to current and future fuel loading. Reintroduction of fire does not consume all large-diameter fuel and generates high levels of surface fuels ≥ 10 cm diameter within 6 years. Repeated fires are needed to reduce surface fuel loading.
Qualitative Assessment of Factors Influencing Implementation Effectiveness and Sustainability of Strategies for Increasing Tobacco Use Treatment in Vietnam Health Centers
Authors: VanDevanter Nancy , Vu Milkie , Nguyen Ann , Nguyen Trang , Van Minh Hoang , Nguyen Nam T. , Shelley Donna R. 
Published: July 2020

PURPOSE Effective strategies are needed to increase the implementation and sustainability of evidence-based tobacco dependence treatment (TDT) in public health systems in low- and middle-income countries. Our cluster randomized controlled trial (VQuit) found that a multicomponent implementation strategy was effective in increasing provider adherence to TDT guidelines in community health centers (CHCs) in Vietnam. In this paper, we present findings from a postimplementation qualitative assessment of factors that influence implementation effectiveness and program sustainability. METHODS We conducted semistructured qualitative interviews (N = 52) with 13 CHC medical directors (ie, physicians), 25 CHC health care providers, and 14 village health workers (VHWs) in 13 study sites. Interviews were transcribed and translated into English. RESULTS Facilitators of implementation effectiveness included training that increased confidence and skill, satisfaction with point-of-service tools, increasing patient demand for TDT, and, for the intervention arm, the value of a VHW referral system that reduced provider burden. The primary challenge to sustainability is the competing priorities driven by the Vietnam Ministry of Health, which may result in fewer resources for TDT compared with other health programs. However, providers and VHWs described several options for adapting the implementation strategies to address challenges and increase the engagement of local government committees and other sectors to sustain gains. CONCLUSION Our findings offer insight into how a multicomponent implementation strategy influenced changes in the delivery of evidence-based TDT. In addition, these results illustrate the dynamic interplay between barriers to and facilitators of sustaining TDT at the policy and community/practice level, particularly in the context of centralized public health systems, like that of Vietnam. Sustaining gains in practice improvement and clinical outcomes will require strategies that include ongoing engagement with policymakers and other stakeholders at the national and local level, as well as planning for adaptations and subsequent resource allocations, to meet Article 14 goals.