bluuebunny/crossref_metadata_2025_split huggingface dataset:
The inhibition of microRNA‐326 by SP1/HDAC1 contributes to proliferation and metastasis of osteosarcoma through promoting SMO expression
Authors: Huang Jiang‐Hu , Xu Yang , Lin Fei‐Yue 
Published: August 2020

AbstractOsteosarcoma (OS) is a malignant bone cancer lacking of effective treatment target when the metastasis occurred. This study investigated the implication of MicroRNA‐326 in OS proliferation and metastasis to provide the clue for the treatment of metastatic OS. This study knocked down SP1 in MG63 and 143B cells and then performed Microarray assay to find the expression of miRNAs that were influenced by SP1. MTT, EdU, wound‐healing and cell invasion assays were performed to evaluated cell proliferation and invasion. OS metastasis to lung was detected in a nude mice model. ChIP assay and DAPA were applied to determine the regulatory effect of SP1 and histone deacetylase 1 (HDAC) complex on miR‐326 expression. Human OS tissues showed lowly expressed miR‐326 but highly expressed Sp1 and HDAC. Sp1 recruited HDAC1 to miR‐326 gene promoter, which caused the histone deacetylation and subsequent transcriptional inhibition of miR‐326 gene. miR‐326 deficiency induced the stimulation of SMO/Hedgehog pathway and promoted the proliferation and invasion of 143B and MG63 cells as well as the growth and metastasis in nude mice. SP1/HDAC1 caused the transcriptional inhibition of miR‐326 gene by promoting histone deacetylation; miR‐326 deficiency conversely stimulated SMO/Hedgehog pathway that was responsible for the proliferation and metastasis of OS.
Association between weight cycling and risk of developing diabetes in adults: A systematic review and meta‐analysis
Authors: Zou Huajie , Yin Ping , Liu Liegang , Duan Wu , Li Pu , Yang Yan , Li Wenjun , Zong Qunchuan , Yu Xuefeng 
Published: August 2020

AbstractAims/IntroductionIn this meta‐analysis, we aimed to explore the association between bodyweight cycling (weight fluctuation) and the risk of developing diabetes.Materials and MethodsWe analyzed data from eligible cohort studies that assessed the association between weight cycling in adults and the risk of developing diabetes from online databases PubMed, Cochrane Library and EMBASE databases (1966 to April 2020). We pooled data using relative risks (RRs) with a random effects model.ResultsA total of 14 studies involving 253,766 participants, including 8,904 diabetes events, were included. One study included eight independent reports, resulting in 21 reports in 14 studies. Summary analysis showed that individuals who suffered weight cycling had a higher risk of diabetes (RR 1.23. 95% confidence interval 1.07–1.41; P = 0.003). However, the association between weight cycling and the risk of developing diabetes was not observed in obese participants (body mass index ≥30 kg/m2; P = 0.08).ConclusionsThe present meta‐analysis showed that weight cycling was a strong independent predictor of new‐onset diabetes. Future studies are required to detect the causal links between weight cycling and the risk of developing diabetes.
Measurement properties of the product of investigator’s global assessment and body surface area in children and adults with atopic dermatitis
Authors: Silverberg J.I. , Lei D. , Yousaf M. , Janmohamed S.R. , Vakharia P.P. , Chopra R. , Chavda R. , Gabriel S. , Patel K.R. , Singam V. , Kantor R. , Hsu D.Y. 
Published: August 2020

AbstractBackgroundMultiple clinician‐reported outcome measures exist for atopic dermatitis (AD) severity. However, there is no gold standard for use in clinical practice.ObjectivesTo determine the measurement properties of the product of validated Investigator's Global Assessment for AD (vIGA) and body surface area (BSA) overall or divided into six categories (cBSA: 0%/0.1, <10%/10, <30%/30, <50%/50, <70%/70 and <90%/90–100%) and compare with other clinician‐reported and patient‐reported outcomes in adults and children with AD.MethodsWe performed a prospective dermatology practice‐based study using questionnaires and evaluation by a dermatologist (n = 653).ResultsvIGA*BSA and vIGA*cBSA had good convergent validity with BSA (Spearman's ρ = 0.97 and 0.93), eczema area and severity index (ρ = 0.94 and 0.92), and objective SCORAD (ρ = 0.88 and 0.89); and weak‐to‐good convergent validity with Numeric Rating Scale average itch (ρ = 0.22 and 0.22) and worst itch (ρ = 0.27 and 0.28), Patient‐Oriented Eczema Measure (ρ = 0.44 and 0.43), Dermatology Life Quality Index (ρ = 0.48 and 0.49), ItchyQOL (ρ = 0.45 and 0.46), PROMIS Sleep Disturbance (ρ = 0.46 and 0.37) and sleep‐related impairment (ρ = 0.31 and 0.31) in adults and/or children; very good discriminant validity for physician‐reported global AD severity; good responsiveness to change of severity of AD and itch; and good reliability (intraclass correlation coefficient [95% confidence interval]: 0.72 [0.60–0.81] and 0.74 [0.62–0.82]) with no floor or ceiling effects. Thresholds for interpretability bands and clinically important difference were established.ConclusionsvIGA*BSA and vIGA*cBSA scores showed good convergent and discriminant validity, reliability, responsiveness and interpretability in adults and children with AD, and were feasible for use in clinical practice. vIGA*BSA and vIGA*cBSA had slightly lower convergent validity than EASI or objective SCORAD, but might be more efficient to collect and score.
IDQoL, CDLQI and the 45‐item CADIS received a sufficient content validity rating during the HOME VII meeting in Japan: a group discussion study
Authors: Gabes M. , Apfelbacher C. ,   for the quality of life working group of the Harmonising Outcome Measures for Eczema (HOME) initiative
Published: August 2020

AbstractBackgroundThe Harmonising Outcome Measures for Eczema (HOME) initiative has agreed that quality of life should be measured in all atopic eczema clinical trials. Various candidate instruments exist for this domain but their content validity in atopic eczema is largely unclear.ObjectiveTo assess the content validity of quality‐of‐life candidate instruments for atopic eczema in infants, children and adults in order to aid the decision on what instrument to include in the core outcome set for the quality‐of‐life domain.MethodsSix group discussions were conducted at the HOME VII Meeting in Tokyo. Each group was composed of 8–12 patients or parents of patients, clinicians, methodologists and pharmaceutical industry delegates and discussed one or two candidate instruments. The COSMIN criteria on relevance, comprehensiveness and comprehensibility were used to determine the overall content validity rating per instrument.ResultsContent validity of the Infant's Dermatitis Quality of Life Index, Children's Dermatology Life Quality Index and the Childhood Atopic Dermatitis Impact Scale (CADIS) long‐form was rated as sufficient (+). Results for the CADIS short‐form, DLQI and Skindex were inconsistent (±). DISABKIDS, Infants and Toddlers Dermatology Quality of Life and ABS‐A were classified as having insufficient content validity.ConclusionsThe content validity rating allowed for a comparison of all candidate instruments and informed the consensus‐seeking process regarding the core instrument for the quality‐of‐life domain.
Fish biodiversity of Saint Peter and Saint Paulʼs Archipelago, Mid‐Atlantic Ridge, Brazil: new records and a species database
Authors: Pinheiro Hudson T. , Macena Bruno C. L. , Francini‐Filho Ronaldo B. , Ferreira Carlos E. L. , Albuquerque Fernanda V. , Bezerra Natalia P. A. , Carvalho‐Filho Alfredo , Ferreira Romulo C. P. , Luiz Osmar J. , Mello Thayna J. , Mendonça Sibele A. , Nunes Diogo M. , Pimentel Caio R. , Pires Alessandra M. A. , Soares‐Gomes Abilio , Viana Danielle L. , Hazin Fabio H. V. , Rocha Luiz A. 
Published: August 2020

AbstractSaint Peter and Saint Paul's Archipelago (SPSPA), one of the smallest and most isolated island groups in the world, is situated on the Mid‐Atlantic Ridge, between Brazil and the African continent. SPSPA has low species richness and high endemism; nonetheless, the diversity of fishes from deep habitats (>30 m depth) had not been previously studied in detail. Several expeditions conducted between 2009 and 2018 explored the shallow and deep reefs of SPSPA using scuba, closed‐circuit rebreathers, manned submersibles, baited remote underwater stereo‐videos (stereo‐BRUV) and fishing between 0 and 1050 m depth. These expeditions yielded 41 new records of fishes for SPSPA: 9 in open waters, 9 in shallow waters (0–30 m), 8 in mesophotic ecosystems (30–150 m) and 15 in deeper reefs (>150 m). Combined with literature records of adult pelagic, shallow and deep‐reef species, as well as larvae, the database of the fish biodiversity for SPSPA currently comprises 225 species (169 recorded as adult fishes and 79 as larvae, with 23 species found in both stages). Most of them (112) are pelagic, 86 are reef‐associated species and 27 are deep‐water specialists. Species accumulation curves show that the number of fish species has not yet reached an asymptote. Whereas the number of species recorded in SPSPA is similar to that in other oceanic islands in the Atlantic Ocean, the proportion of shorefishes is relatively lower, and the endemism level is the third highest in the Atlantic. Twenty‐nine species are listed as threatened with extinction. Observations confirm the paucity of top predators on shallow rocky reefs of the island, despite the presence of several pelagic shark species around SPSPA. Because all of the endemic species are reef associated, it is argued that the new marine‐protected areas created by the Brazilian government do not ensure the protection and recovery of SPSPA's biodiversity because they allow exploitation of the most vulnerable species around the archipelago itself. This study suggests a ban on reef fish exploitation inside an area delimited by the 1000 m isobath around the islands (where all known endemics are concentrated) as the main conservation strategy to be included in the SPSPA management plan being prepared by the Brazilian government.
The relationship between oral‐origin bacteria in the fecal microbiome and albumin–bilirubin grade in patients with hepatitis C
Authors: Yamamoto Kenta , Honda Takashi , Ito Takanori , Ishizu Yoji , Kuzuya Teiji , Nakamura Masanao , Miyahara Ryoji , Kawashima Hiroki , Ishigami Masatoshi , Fujishiro Mitsuhiro 
Published: August 2020

AbstractBackgroundBacteria of oral origin (BO) in the gut are associated with prognosis in patients with cirrhosis. The Greengenes database (gg_13_8) is widely used in microbiome analysis, but the expanded Human Oral Microbiome Database (eHOMD), a specialized database for BO, can add more detailed information. We used each database to evaluate the relationship between the albumin–bilirubin grade (ALBI) and the microbiome in patients with hepatitis C.MethodsEighty patients were classified into the low ALBI group (LA; n = 34) or high ALBI group (HA; n = 46). Isolated DNA from stool was amplified to target the V3–4 regions of 16S rRNA. The microbiomes of the two groups were compared using gg_13_8 or eHOMD. We evaluated the associations between microbiomes and prognoses using Cox proportional hazards models.ResultsAt the genus level, the two groups differed significantly regarding 6 (gg_13_8) and 7 (eHOMD) types of bacteria. All types except Akkermansia are classified as BO. Both databases showed an increase in Streptococcus and Veillonella. eHOMD showed a decrease in Fusobacterium and an increase in Fretibacterium; both produce various types of short‐chain fatty acids. At the species level, the two groups demonstrated significant differences in 2 (gg_13_8) and 6 (eHOMD) bacterial types. Selenomonas noxia and Streptococcus salivarius were related to poor prognosis in univariate analysis.ConclusionThe HA group demonstrated increased BO, most of which produce lactic acid or acetic acid. The correlation between the microbiome and metabolism might be related to prognosis. eHOMD was a useful database for analyzing BO.
Current Trends in Nursing Research Across Five Locations: The United States, South Korea, Taiwan, Japan, and Hong Kong
Authors: Im Eun‐Ok , Sakashita Reiko , Lin Chia‐Chin , Lee Tae‐Hwa , Tsai Hsiu‐Min , Inouye Jillian 
Published: August 2020

AbstractPurposeDespite the importance of research in the discipline of nursing, current trends in nursing research have rarely been discussed across countries. The purpose of this article was to identify current trends in nursing research across five countries, including the United States, South Korea, Taiwan, Japan, and Hong Kong, in order to provide directions for future global nursing research.Organizing ConstructA discussion article.MethodsTo identify the current trends, seven leaders from the five countries had discussions through a series of workshops and conference presentations. After the most recent conference, all the leaders reflected for a month on their presentations and compiled the exemplars and cases from their experience and the existing literature in individual countries into a table. The tables and supporting references were collected at the completion of the reflection period. Then, the PowerPoint (Microsoft Corporation, Redmond, WA, USA) slideshows of the conference presentations by the leaders and the collected tables were analyzed using a content analysis.FindingsSix themes reflecting the current trends in nursing research were extracted: (a) demographic alterations; (b) increasing diversities and globalization; (c) technology innovation; (d) individualized or personal care and population health initiatives; (e) health policies and regulations; and (f) nursing workforce changes.ConclusionsFuture directions for nursing research across the countries were proposed: (a) cost‐effectiveness research; (b) implementation science; (c) data science; (d) training of the future generation of nurse researchers; (e) population health; and (f) team science.Clinical RelevanceThis topic could be applied to any clinical settings.
Botulinum toxin as a double‐edged sword in alopecia: A systematic review
Authors: Wang Yuanzhuo , Zhang Hanlin , Zheng Qingyue , Tang Keyun , Fang Rouyu , Sun Qiuning 
Published: August 2020

AbstractBackgroundAlopecia is a common disease influencing the quality of life of the patients. Botulinum toxin has been widely employed in cosmetic dermatology and was applied in some clinical trials to treat alopecia.AimsHence, We conducted a systematic review to examine the safety and efficacy of using botulinum toxin in treating alopecia.MethodsCochrane Library, Embase, PubMed, and Web of Science databases were used to search for articles. A PICOS question was formed and the author, year of publication, number of participants, study design, product, dilution and dosing, outcome measurement, findings, side effects, and follow‐up were retrieved. The Oxford Centre for Evidence‐Based Medicine 2011 Levels of Evidence was used as a qualitative indicator to evaluate the levels of evidence.ResultsEleven articles published between 2005 and 2019 were included. 9 of which were about treatment effects and 2 were concerning adverse effects. The total number of the participants was 106, and the number for each article was ranged from 1 to 50. Varying type of outcome measurements was used for the publications. 8 articles were evaluated as Level 4 and 1 was evaluated as Level 5 during the 9 articles with treatment effects.ConclusionsThe results showed that botulinum toxin may function as a double‐edged sword and have a low degree of efficacy and safety in treating alopecia. The study may be useful to remind the physicians of adverse effects in clinical practice and pave the way for further research. A larger, randomized, placebo‐controlled study is warranted.
Vascular risk factor control and adherence to secondary preventive medication after ischaemic stroke
Authors: Gynnild M. N. , Aakerøy R. , Spigset O. , Askim T. , Beyer M. K. , Ihle‐Hansen H. , Munthe‐Kaas R. , Knapskog A. B. , Lydersen S. , Næss H. , Røsstad T.G. , Seljeseth Y. M. , Thingstad P. , Saltvedt I. , Ellekjær H. 
Published: August 2020

AbstractBackgroundStudies regarding adequacy of secondary stroke prevention are limited. We report medication adherence, risk factor control and factors influencing vascular risk profile following ischaemic stroke.MethodsA total of 664 home‐dwelling participants in the Norwegian Cognitive Impairment After Stroke study, a multicenter observational study, were evaluated 3 and 18 months poststroke. We assessed medication adherence by self‐reporting (4‐item Morisky Medication Adherence Scale) and medication persistence (defined as continuation of medication(s) prescribed at discharge), achievement of guideline‐defined targets of blood pressure (BP) (<140/90 mmHg), low‐density lipoprotein cholesterol (LDL‐C) (<2.0 mmol L−1) and haemoglobin A1c (HbA1c) (≤53 mmol mol−1) and determinants of risk factor control.ResultsAt discharge, 97% were prescribed antithrombotics, 88% lipid‐lowering drugs, 68% antihypertensives and 12% antidiabetic drugs. Persistence of users declined to 99%, 88%, 93% and 95%, respectively, at 18 months. After 3 and 18 months, 80% and 73% reported high adherence. After 3 and 18 months, 40.7% and 47.0% gained BP control, 48.4% and 44.6% achieved LDL‐C control, and 69.2% and 69.5% of diabetic patients achieved HbA1c control. Advanced age was associated with increased LDL‐C control (OR 1.03, 95% CI 1.01 to 1.06) and reduced BP control (OR 0.98, 0.96 to 0.99). Women had poorer LDL‐C control (OR 0.60, 0.37 to 0.98). Polypharmacy was associated with increased LDL‐C control (OR 1.29, 1.18 to 1.41) and reduced HbA1c control (OR 0.76, 0.60 to 0.98).ConclusionRisk factor control is suboptimal despite high medication persistence and adherence. Improved understanding of this complex clinical setting is needed for optimization of secondary preventive strategies.
Influence of High‐Pressure Polymerization on Mechanical Properties of Denture Base Resins
Authors: Becerra José , Mainjot Amélie , Hüe Olivier , Sadoun Michaël , Nguyen Jean‐François 
Published: August 2020

AbstractPurposeThe purpose of this study was to study the influence of high‐pressure (HP) polymerization on the mechanical properties of denture base PMMA resins compared with conventional thermopolymerization and PMMA discs for digital dentures.Materials and MethodsThree groups of blocks were prepared: Probase Hot (Ivoclar Vivadent, Lichtenstein) conventionally heat polymerized at 100°C, Probase Hot heat polymerized at 100°C under HP (200 MPa) and Ivobase CAD (Ivoclar Vivadent, Lichtenstein). Samples for mechanical/physical (n = 30) and samples for viscoelastic (n = 10) characterizations were cut from the blocks. Flexural strength (σf), elastic modulus (Ef), hardness, density (ρ), flexural deformation at maximal flexural stress, flexural load energy (Ur) and viscoelastic properties (E’, E’’, Tanδ, Tg) were analyzed using one‐way ANOVA (α = 0.05), Scheffé multiple means comparisons (α = 0.05) and Weibull statistics (for σf). SEM images of the fractured surfaces were obtained.ResultsEf, E’, E’’ and density of HP polymerized Probase hot were significantly higher than conventional heat polymerized Probase Hot, whereas Tg was significantly lower and σf, Tanδ, hardness, flexural deformation at maximal flexural stress, Ur were not significantly different. The highest values for σf, flexural deformation at maximal flexural stress, Ur and Weibull modulus were obtained with Ivobase CAD.ConclusionHP polymerization does not significantly increase the mechanical properties of denture base resins.
Reflecting on nostalgic, positive, and novel experiences increases state Openness
Authors: Hotchin Victoria , West Keon 
Published: August 2020

AbstractObjectivePersonality change is a growing field of interest, but relatively few studies have examined causes of change in Openness. We investigated whether it is possible to influence state Openness, and through what mechanisms this effect may occur.MethodIn two experiments (Study 1: N = 144, Mage = 36.4, 58% female, 88% White; Study 2: N = 269, Mage = 34.0, 60% female, 91% White), participants reflected on and wrote about a personal experience requested to be either: nostalgic; positive and novel (Study 1); positive or novel (Study 2); or ordinary. They rated the events for nostalgia, positivity, novelty, and sociality, and completed measures of state positive affect, self‐esteem, social connectedness, meaning in life, and state Openness.ResultsParticipants who recalled positive and/or novel events reported greater state Openness than those who recalled ordinary events. In Study 1, this also applied to those recalling nostalgic events. Event ratings of positivity (both studies), nostalgia and novelty (Study 2) independently predicted state Openness. State positive affect and self‐esteem were independent predictors in both studies, suggesting possible indirect paths.ConclusionsReflecting on nostalgic, positive, and novel experiences can increase state Openness. This finding may be useful for interventions targeting trait‐level change.
Neuroticism mediates the relationship between industrial history and modern‐day regional obesity levels
Authors: Daly Michael , Obschonka Martin , Stuetzer Michael , Sutin Angelina R. , Shaw‐Taylor Leigh , Satchell Max , Robinson Eric 
Published: August 2020

AbstractObjectiveThe historical factors and contemporary mechanisms underlying geographical inequalities in obesity levels remain uncertain. In this study, we examine whether modern regional variation in obesity is partly a result of the impact of large‐scale industry on the personality traits of those living in regions once at the center of the Industrial Revolution.MethodExposure to the effects of the Industrial Revolution was assessed using unique historical data from English/Welsh counties (N = 111). Specifically, we examined the relationship between the regional employment share in large‐scale coal‐based industries in 1813–1820 and contemporary regional obesity levels (2013–2015). The Big Five personality traits and regional unemployment levels were examined as potential mediators of this association.ResultsThe historical regional employment share in large‐scale industries positively predicted the modern‐day regional prevalence of obesity. Mediation analysis showed that areas exposed to the decline of large‐scale industries experienced elevated Neuroticism and unemployment levels that explained almost half of the association between the historical dominance of large‐scale industry and modern‐day obesity levels.ConclusionsOur results provide initial evidence that raised regional Neuroticism levels may play a key role in explaining why exposure to the rapid growth and subsequent decline of large‐scale industries forecasts modern‐day obesity levels.