bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct effects of PTST2b and MRC on starch granule morphogenesis in potato tubers
Authors: Hochmuth Anton , Carswell Matthew , Rowland Aaron , Scarbrough Danielle , Esch Lara , Kamble Nitin Uttam , Habig Jeffrey W. , Seung David 
Published: December 2024

SummaryThe molecular mechanisms underpinning the formation of the large, ellipsoidal starch granules of potato tuber are poorly understood. Here, we demonstrate the distinct effects of PROTEIN TARGETING TO STARCH2b (PTST2b) and MYOSIN RESEMBLING CHLOROPLAST PROTEIN (MRC) on tuber starch granule morphology. A gene duplication event in the Solanaceae resulted in two PTST2 paralogs (PTST2a and PTST2b). PTST2b is expressed in potato tubers, and unlike PTST2a, it had no detectable interaction with STARCH SYNTHASE 4. MRC expression was detectable in leaves, but not in tubers. Using transgenic potato lines in the variety Clearwater Russet, we demonstrate that MRC overexpression leads to the formation of granules with aberrant shapes, many of which arise from multiple initiation points. Silencing PTST2b led to the production of striking near‐spherical granules, each arising from a single, central initiation point. Contrary to all reported PTST2 mutants in other species, we observed no change in the number of granules per cell in these lines, suggesting PTST2b is specifically involved in the control of starch granule shape. Starch content and tuber yield per plant were not affected by PTST2b silencing, but MRC overexpression led to strong decreases in both parameters. Notably, the spherical granules in PTST2b silencing lines had a distinctively altered pasting profile, with higher peak and final viscosity than the wild type. Thus, PTST2b and MRC are promising target genes for altering starch granule size and shape in potato tubers, and can be used to create novel starches with altered physicochemical and/or functional properties.
Dental Abnormalities in Congenital Ichthyoses: Case Report and Review of the Literature
Authors: Maarouf Sarah , Clark Marie , Chen Anthony , Haggstrom Anita 
Published: December 2024

ABSTRACTWe describe a 1‐day old female with features of keratitis‐ichthyosis‐deafness (KID) syndrome and natal teeth. Genetic analysis confirmed GJB2 263C and A88V de novo pathogenic variants consistent with KID syndrome. Natal teeth were promptly extracted to avoid the risk of aspiration. This review describes subsets of ichthyoses that have been reported in association with dental anomalies, highlighting the need for early dental referral and importance of long‐term follow‐up.
Modelling photosynthetic responses by day and night during initial water stress in Pulmonaria vallarsae
Authors: Pupillo Paolo , Sparla Francesca , Melandri Bruno Andrea , Trost Paolo 
Published: December 2024

AbstractThe relationships between photosynthesis and initial water deficit stress were investigated by chlorophyll fluorescence analysis in Pulmonaria vallarsae, a shade tolerant, perennial C3 herb by following changes of light response curves (LRCs) in single leaves during water shortage. We devised an LRC model based on two interacting rectangular hyperbolae (DH model) for the low (H1) and the high irradiance regions (H2), characterized by two parameters: maximum extrapolated ETR (V1, V2) and half‐saturation irradiance (K1, K2). While H1 is assumed to represent an ETR‐related function, H2 may reflect Rubisco activity. Plants were subjected to four days of water restriction in summer and tested every 12 h. While daytime values remained relatively stable, increasing water stress gradually induced a night depression of photosynthesis mainly dependent on decreasing ETR with concomitant reduction of PSII‐dependent parameters (ΦPSII) and fluorescence‐related functions, while nonphotochemical quenching (NPQ) was strongly enhanced. In terms of the DH model, the night depression of photosynthesis featured a night drop of V2 and K2 followed by decreases of V1 and K1. The H2 hyperbola was more stress responsive than H1 and frequently showed a reversible decrease of nocturnal H2 parameters (bright illumination constraint, BIC). Pulmonaria plants tested during winter with very low water stress displayed LRCs resembling rectangular hyperbolae, similar during day and night. The DH model is shown to yield accurate and perspicuous photosynthetic parameters representing the principal components of an LRC and to be well suitable to document the day/night divergence of photosynthetic capacity during a water deficit stress.
Reconstructing Identity and Purpose in the Athlete-to-Coach Transition: A Cross-Case Analysis of Two Contrasting Division I Graduate Assistant Coaches in the United States
Authors: Yukhymenko-Lescroart Mariya A. 
Published: December 2024

The pathway to becoming a coach often begins with playing the sport as an athlete; yet, the athlete-to-coach transition often presents challenges concerning self-comprehension, encompassing both one’s sense of self and overall purpose in life. Through a qualitative case study approach, this study sought to examine the athlete-to-coach transition experiences of two contrasting Division I graduate assistant coaches in the United States: one in men’s football and one in women’s softball. Employing in-depth interviews and thematic analysis, a “case-based” approach followed by a cross-case synthesis showed the main themes of (a) athlete-to-coach transition experiences (process, challenges, and the role of athletic experiences); (b) identity transformation (as an athlete, postathletic redefinition of self, emerging identity as a coach, and major shifts in self-views during each major phase); and (c) shifts in life purpose (process, challenges, and orientation: self vs. others focused). Recommendations for supporting graduate assistant coaches are discussed.
Early Molecular Response to Imatinib First‐Line Therapy and Predictive Factors of Poor Outcomes for Chronic Myeloid Leukemia Patients in Côte d’Ivoire
Authors: Koffi Kouassi Gustave , Bognini Sara Akou , Silué Dohoma Alexis , Kamara Ismael , Kouakou Ines , N’dhatz Emeraude , Kouakou Boidy , Nanho Danho Clotaire , Okou David Tea 
Published: December 2024

Objective: The present study aimed to evaluate for the first time, the early molecular response (EMR) to imatinib at 3 months for patients with chronic myeloid leukemia and to determine the predictive factors that influence poor outcome and response.Methods: 60 newly diagnosed CML patients were enrolled from May 2018 to June 2023. They received imatinib and prospectively underwent a molecular evaluation. Their EMR was assessed using a RT‐qPCR method and expressed as the BCR::ABL1 IS transcript level at 3 months. Potential factors impacting the EMR were identified using the Cox proportional hazard regression models. The effects of an EMR on the cumulative incidence of a deep molecular response (DMR) were also evaluated.Results: Out of the 60 CML patients recruited, 29 (48%) achieved an optimal response with TKI therapy after 3 months. The cumulative rate of molecular response was 16 (36%) for a major molecular response (MMR), 10 (23%) for MR4, 8 (18%) for MR4.5, and 6 (14%) for MR5, while 4 (9%) showed indetectable transcript. In addition, as 26 (90%) of patients with optimal response at 3 months showed a DMR, we determined that an optimal response to TKI at 3 months was significantly correlated with a DMR. We also identified through multivariate analysis that seven independent risk factors significantly influenced an EMR to TKI. These factors included male, late diagnosis, advanced performance status, the presence of splenomegaly, high‐ELTS risk groups, a BCR::ABL1 domain mutation, and complete hematologic response after more than 30 days.Conclusion: Our study demonstrates that an EMR at 3 months has a predictive value for a DMR. In addition, a MMR and a DMR can be predicted using a combination of parameters that either have a significant impact on the optimal response, or that can serve as prognostic indicators for molecular response, especially in low‐income countries, where molecular assessment and monitoring are not available or possible.
Lourdes Sola. Ideias econômicas, decisões políticas: técnicos e políticos no governo da economia
Authors: Ribeiro Ivy Renee Augusto , Giannecchini Laura 
Published: December 2024

Book review: Ideias econômicas, decisões políticas: técnicos e políticos no governo da economia, São Paulo, Edusp, 2023, by Lourdes Sola.
A long-term follow-up study of the changes in localized intravascular coagulation-related indexes after sclerotherapy of venous malformation
Authors: Zheng Miao , Zhong Hai-Yan , Sun Yi-Ran , Zou Ming-Li , Chen Yong-Pei , Yuan Si-Ming 
Published: December 2024

Objective Localized intravascular coagulation (LIC) is a unique phenomenon associated with venous malformations (VMs). This study investigated the changes in LIC related indexes following sclerotherapy of VMs and its correlation with therapeutic efficacy. Methods From August 2019 to September 2023, patients who met the following criteria were enrolled into this study: (1) individuals with venous malformations accompanied by LIC; (2) those who had undergone a minimum of three sclerotherapy sessions; (3) blood coagulation function tests were conducted prior to each treatment; and (4) magnetic resonance imaging (MRI) scans were conducted both before and after three times of treatment. Those who fail to meet with the previous inclusion criteria were excluded. The coagulation profile prior to each sclerotherapy treatments was assessed. The therapeutic outcomes were evaluated at the end of treatment. Results A total of 21 patients were enrolled into the study, with the age ranging from 4 to 61 years old. Elevated D-dimer levels, with or without a decrease in fibrinogen (FIB) and an increase in fibrin degradation products (FDP) are clinical features of VMs with LIC. The coagulation indexes tend to settle down with several sclerotherapy treatments. There were 5 cases in the significantly improved group, 13 cases in the improved group, and 3 cases in the no improved group when it came to efficacy assessment. The better therapeutic efficacy is more pronounced the improvement of LIC. Conclusions LIC gradually returns to normal during sclerotherapy, and the degree of recovery is directly related to the treatment’s efficacy. One useful metric to evaluate the effectiveness of sclerotherapy for VMs is the improvement of LIC.
Effects of small incision cataract surgery and routine ultrasonic phacoemulsification on vision recovery, anterior segment parameters and corneal regularity
Authors: Zhu Yueqing , Liu Weijie , Liang Yong 
Published: December 2024

Objective To compare the effects of minor incision cataract surgery (SICS) and routine ultrasonic phacoemulsification (PHACO) on vision recovery, anterior segment parameters and corneal regularity. Methods Ninety-two age-related cataract patients (100 eyes) were prospectively selected from January 2017 to December 2019. Fifty cases (50 eyes) were treated with SICS, while the remaining 42 cases (50 eyes) underwent PHACO. Their post-operative visual acuity, central corneal thickness (CCT), endothelial cell density, coefficient of variation and proportion of hexagonal cells were compared. Results CCT was more extensive in the two groups one week after the operation than before, while it returned to the pre-operative level 6 weeks after. Compared with before the operation, CCT had a significant difference in the two groups one week after the operation (P < 0.05). In contrast, there was no significant difference six weeks after the operation (P > 0.05). CCT had a substantial difference between the two groups 1-week after operation (P < 0.05). The endothelial cell density had a significant difference in PHACO group 1 and 6 weeks after operation compared to before (P < 0.01). In contrast, the two groups had no significant difference at each time point before and after operation (P > 0.05). There were no significant differences in the coefficient of corneal endothelial cell area variation or proportion of hexagonal cells between the two groups at each time point (P > 0.05). Conclusions SICS and PHACO have comparable clinical effects. For patients with senile hard-nucleus cataracts, SICS is characterised by fewer surgical complications.
Transient patterns of advanced brain ageing in female adolescents with anorexia nervosa
Authors: Stratton Tatiana , Bahnsen Klaas , Geisler Daniel , Bernardoni Fabio , Gaser Christian , Ehrlich Stefan , Walton Esther 
Published: December 2024

BackgroundAnorexia nervosa is a psychiatric disorder characterised by undernutrition, significantly low body weight and large, although possibly transient, reductions in brain structure. Advanced brain ageing tracks accelerated age-related changes in brain morphology that have been linked to psychopathology and adverse clinical outcomes.AimThe aim of the current case–control study was to characterise cross-sectional and longitudinal patterns of advanced brain age in acute anorexia nervosa and during the recovery process.MethodMeasures of grey- and white-matter-based brain age were obtained from T1-weighted magnetic resonance imaging scans of 129 acutely underweight female anorexia nervosa patients (of which 95 were assessed both at baseline and after approximately 3 months of nutritional therapy), 39 recovered patients and 167 healthy female controls, aged 12–23 years. The difference between chronological age and grey- or white-matter-based brain age was calculated to indicate brain-predicted age difference (BrainAGEGM and BrainAGEWM).ResultsAcute anorexia nervosa patients at baseline, but not recovered patients, showed a higher BrainAGEGM of 1.79 years (95% CI [1.45, 2.13]) compared to healthy controls. However, the difference was largely reduced for BrainAGEWM. After partial weight restoration, BrainAGEGM decreased substantially (beta = −1.69; CI [−1.93, −1.46]). BrainAGEs were unrelated to symptom severity or depression, but larger weight gain predicted larger normalisation of BrainAGEGM in the longitudinal patient sample (beta = −0.65; CI [−0.75, −0.54]).ConclusionsOur findings suggest that in patients with anorexia nervosa, undernutrition is an important predictor of advanced grey-matter-based brain age, which itself might be transient in nature and largely undetectable after weight recovery.
Early antibiotic exposure and risk of psychiatric and neurocognitive outcomes: systematic review and meta-analysis
Authors: Green Jessica Emily , Wrobel Anna , Todd Emma , Marx Wolfgang , Berk Michael , Lotfaliany Mojtaba , Castle David , Cryan John F. , Athan Eugene , Hair Christopher , Nierenberg Andrew A. , Jacka Felice N. , Dawson Samantha 
Published: December 2024

Background
The prenatal and early-life periods pose a crucial neurodevelopmental window whereby disruptions to the intestinal microbiota and the developing brain may have adverse impacts. As antibiotics affect the human intestinal microbiome, it follows that early-life antibiotic exposure may be associated with later-life psychiatric or neurocognitive outcomes.

Aims
To explore the association between early-life (in utero and early childhood (age 0–2 years)) antibiotic exposure and the subsequent risk of psychiatric and neurocognitive outcomes.

Method
A search was conducted using Medline, PsychINFO and Excerpta Medica databases on 20 November 2023. Risk of bias was assessed using the Newcastle-Ottawa scale, and certainty was assessed using the grading of recommendations, assessment, development and evaluation (GRADE) certainty assessment.

Results
Thirty studies were included (n = 7 047 853 participants). Associations were observed between in utero antibiotic exposure and later development of autism spectrum disorder (ASD) (odds ratio 1.09, 95% CI: 1.02–1.16) and attention-deficit hyperactivity disorder (ADHD) (odds ratio 1.19, 95% CI: 1.11–1.27) and early-childhood exposure and later development of ASD (odds ratio 1.19, 95% CI: 1.01–1.40), ADHD (odds ratio 1.33, 95% CI: 1.20–1.48) and major depressive disorder (MDD) (odds ratio 1.29, 95% CI: 1.04–1.60). However, studies that used sibling control groups showed no significant association between early-life exposure and ASD or ADHD. No studies in MDD used sibling controls. Using the GRADE certainty assessment, all meta-analyses but one were rated very low certainty, largely owing to methodological and statistical heterogeneity.

Conclusions
While there was weak evidence for associations between antibiotic use in early-life and later neurodevelopmental outcomes, these were attenuated in sibling-controlled subgroup analyses. Thus, associations may be explained by genetic and familial confounding, and studies failing to utilise sibling-control groups must be interpreted with caution. PROSPERO ID: CRD42022304128
Climate change and polar marine invertebrates: life-history responses in a warmer, high CO2 world
Authors: Byrne Maria , Lamare Miles D. 
Published: December 2024

ABSTRACT
Polar marine invertebrates serve as bellwethers for species vulnerabilities in the face of changing climate at high latitudes of the Earth. Ocean acidification, warming/heatwaves, freshening, sea ice retreat and productivity change are challenges for polar species. Adaptations to life in cold water with intensely seasonal productivity has shaped species traits at both poles. Polar species have life histories often characterised as K-strategist or K-selected (e.g. slow growth and development, larval hypometabolism) that make them sensitive to climate stress and altered seasonal productivity. Moderate warming results in faster development and can have positive effects on development, up to a limit. However, ocean acidification can retard development, impair skeletogenesis and result in smaller larvae. Given the fast pace of warming, data on the thermal tolerance of larvae from diverse species is urgently needed, as well as knowledge of adaptive responses to ocean acidification and changes to sea ice and productivity. Predicted productivity increase would benefit energy-limited reproduction and development, while sea ice loss negatively impacts species with reproduction that directly or indirectly depend on this habitat. It is critical to understand the interactive effects between warming, acidification and other stressors. Polar specialists cannot migrate, making them susceptible to competition and extinction from range-extending subpolar species. The borealisation and australisation of Arctic and Antarctic ecosystems, respectively, is underway as these regions become more hospitable for the larval and adult life-history stages of lower-latitude species. Differences in biogeography and pace of change point to different prospects for Arctic and Antarctic communities. In this Commentary, we hypothesise outcomes for polar species based on life history traits and sensitivity to climate change and suggest research avenues to test our predictions.
Fully Polynomial Time Approximation Schemes for Robust Multistage Decision Making
Authors: Halman Nir , Nannicini Giacomo 
Published: December 2024

We design a framework to obtain Fully Polynomial Time Approximation Schemes (FPTASes) for adjustable robust multistage decision making under the budgeted uncertainty sets introduced by Bertsimas and Sim. We apply this framework to the robust counterpart of three problems coming from operations research: (i) ordered knapsack, (ii) single-item inventory control, and (iii) single-item batch dispatch. Our work gives the first FPTAS for these problems, and for adjustable robust multistage decision making in general. The proposed approximation schemes are constructed with the technique of K-approximation sets and functions, relying on careful robust dynamic programming formulations for a master problem (corresponding to the decision maker) and for an adversary problem (corresponding to nature, which chooses bad realizations of uncertainty for the decision maker). The resulting algorithms are short and simple, requiring just a few concise subroutines. History: Accepted by Andrea Lodi, Area Editor for Design & Analysis of Algorithms—Discrete. Funding: This research was supported in part by the United States-Israel Binational Science foundation [Grant 2018095). N. Halman was also supported in part by the Israel Science Foundation [Grants 399/17 and 1074/21].
Produção e qualidade de frutos de melão submetido a adubação orgânica e mineral em ambiente protegido
Authors: Lima Hedilberto Carmo de , Cordovil Hellen Patricia Lemos , Oliveira Cássio Freitas de , Costa Karla Samylle de Queiroz , Melo Madson Pereira , Silva Josiane Gonçalves , Melo Nilvan Carvalho 
Published: December 2024

O melão desempenha grande importância para a agricultura brasileira, porém seu cultivo ainda é pouco estudado, principalmente no que diz respeito à adubação. O cultivo em sistema orgânico vem ganhando espaço, minimizando os gastos com insumos agrícolas. Assim, objetivou-se estudar a influência da adubação orgânica e mineral na produção e qualidade dos frutos de meloeiro cultivados em casa de vegetação no município de Porto Grande/AP. O delineamento experimental foi em blocos casualizados, com quatro tratamentos: T0 = controle; T1 = adubação orgânica; T2 = adubação orgânica + adubação química e T3 = adubação química (NPK na formulação: 10-28-20). A semeadura foi realizada, em copos plásticos de 150 ml, contendo substrato com húmus de minhoca. O transplantio foi realizado sob plástico mulching 15 dias após a germinação, adotando-se o sistema de irrigação por gotejamento. A colheita dos frutos de melão ocorreu aos 60 dias após o transplantio, sendo analisados parâmetros, como: comprimento do fruto; diâmetro do fruto; peso do fruto e teor de sólidos solúveis (°Brix). O único tratamento que não diferiu dos demais foi o controle, os demais tratamentos são iguais. A adubação orgânica promove maior produção e qualidade dos frutos de melão, em relação ao tratamento controle, cultivados em ambiente protegido sob o sistema de plástico mulching. A adubação organomineral apresenta potencial para a produção de melão.
Scapular metastasis from acinic cell carcinoma of parotid gland: A case report
Authors: Sun Deng-Qun , Chen Chang-Chun , Zheng Duo-An , Xing Hai-Yan , Peng Xue 
Published: December 2024

BACKGROUND
Acinic cell carcinoma (ACC) is a malignant epithelial neoplasm that commonly occurs in the parotid gland. It is known to have a high recurrence rate and the potential to metastasize to the lung or cervical lymph nodes. However, few cases of ACC with bone metastasis have been reported in the medical literature.
CASE SUMMARY
The clinical significance of this case report lies in the unique site of occurrence of the metastasis: To the best of our knowledge, this report is the only literature documenting ACC arising in a shoulder mass.
CONCLUSION
Unusual presentations of uncommon malignancies can present diagnostic challenges for both surgeons and histopathologists. It is important to be aware of these rare occurrences in order to provide the best possible treatment for patients.