bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between Single-Nucleotide Polymorphisms in Breast Cancer Susceptibility Genes and Clinicopathological Characteristics
Authors: Zhang Kejing , Tang Lili , Wang Shouman , Yang Yuan , Wang Hao , Zhou Zhiyang , Pang Jian , Chen Feiyu 
Published: June 2020

Abstract
Objective

The purpose of the present study was to evaluate the associations between seven tagging single nucleotide polymorphisms (tSNPs) and risk of breast cancer assessed by tumor pathological characteristics and body mass index (BMI).
Methods

Seven tSNPs of four breast cancer susceptibility genes were analyzed in 734 Chinese women with breast cancer and 672 age-matched healthy controls, then the association with clinicopathological characteristics, BMI, molecular subtype, TNM staging and lymph node status, was determined.
Results

Two tSNPs, rs12951053 located in TP53 and rs16945628 in BRIP1, displayed increased risk of breast cancer in the BMI ≧ 25 kg/m2 group (OR = 1.50, 95% CI: 1.02–2.21, P = 0.041 and OR = 1.92, 95% CI: 1.13–3.26, P = 0.015, respectively). The other five tSNPs (rs1805812, rs2735385 and rs6999227 in NBS1, rs7220719 in BRIP1 and rs2299941 in PTEN) displayed a decreased risk of breast cancer in the 18.5 ≤ BMI < 25 kg/m2 group. Two tSNPs, rs12951053 in TP53 and rs7220719 in BRIP1 exhibited an increased risk of triple-negative breast cancer(TNBC) (OR = 1.50, 95% CI: 1.05–2.15, P = 0.026 and OR = 2.13, 95% CI: 1.05–4.29, P = 0.032, respectively), and the three tSNPs in NBS1 (rs1805812, rs2735385 and rs6999227) all displayed negative association with both luminal B breast cancer and TNBC. The tSNP rs2299941 in PTEN also exhibited a negative association with each breast cancer molecular subtype, except TNBC. The majority of tSNPs displayed a negative association with stage II or III breast cancer. A number of tSNPs showed a negative association with breast cancer that was lymph node negative or with 1–3 positive nodes. Only one tSNP, rs12951053 in TP53 displayed a positive association with lymph node negative breast cancer (OR = 1.43, 95% CI: 1.08–1.91, P = 0.013).
Conclusions

The majority of tSNPs displayed a negative association with breast cancer and only a few tSNPs (rs12951053 in TP53, rs16945628 and rs7220719 in BRIP1) showed an increased risk of breast cancer as defined by clinicopathological characteristics.
Opportunities and challenges to Integrating mental health into HIV programs in a Low- and Middle-Income Country: Insights from the Nigeria Implementation Science Alliance
Authors: Ezeanolue Echezona E , Iheanacho Theddeus , Adedeji Isaac , Itanyi Ijeoma , Olakunde Babayemi , Patel Dina , Dakum Patrick , Okonkwo Propser , Akinmurele Timothy , Obiefune Michael , Khamofu Hadiza , Oyeledun Bolanle , Aina Muyiwa , Eyo Andy , Oleribe Obinna , Oko John , Olutola Ayodotun , Gobir Ibrahim , Aliyu Muktar H , Aliyu Gambo , Woelk Godfrey , Aarons Gregory , Siberry George , Sturke Rachel , ASSISTANT NISA 
Published: June 2020

Abstract
Background: In Nigeria, there is an estimated 1.9 million people living with HIV (PLHIV), 53% of whom utilize HIV care and services. With decreasing HIV-related deaths and increasing new infections, HIV with its associated comorbidities continue to be a key public health challenge in Nigeria. Untreated, comorbid mental disorders are a critical but potentially modifiable determinant of optimal HIV treatment outcomes. This study aimed to identify the challenges and opportunities related to integrating mental health care into existing HIV programs in Nigeria.Method: Attendees at the Nigeria Implementation Science Alliance (NISA)’s 2019 conference participated in nominal group technique (NGT) exercise informed by the “Exploration, Preparation, Implementation, and Sustainment (EPIS)” framework. The NGT process was conducted among the nominal groups in two major sessions of 30-minutes phases followed by a 30-minute plenary session. Data analysis proceeded in four steps: transcription, collation, theming and content analysis.Results: The two major theoretical themes from the study were – opportunities and challenges of integrating mental health treatment into HIV services. Three sub-themes emerged on opportunities: building on health care facilities for HIV services (screening, counseling, task-sharing monitoring and evaluation frameworks) , utilizing existing human resources or workforce in HIV programs (in-service training and including mental health in education curriculum) and the role of social and cultural structures (leveraging existing community, traditional and faith-based infrastructures). Four sub-themes emerged for challenges: double burden of stigma and the problems of early detection (HIV and mental health stigma, lack of awareness) , existing policy gaps and structural challenges (fragmented health system) , limited human resources for mental health care in Nigeria (knowledge gap and burnout) and dearth of data/evidence for planning and action (research gaps) .Conclusions: Potential for integrating treatments for mental disorders into HIV services exist in Nigeria. These include opportunities for clinicians’ training and capacity building as well as community partnerships. Multiple barriers and challenges such as stigma, policy and research gaps would need to be addressed to leverage these opportunities. Our findings serve as a useful guide for government agencies, policy makers and research organizations to address co-morbid mental disorders among PLHIV in Nigeria.
Conprehensive Analysis of circRNA-microRNA-mRNA Network Revealed Novel Regulatory Mechanism in Trichosposon Asahii Infection
Authors: Zhang Ming-Wang , Zhu Zhi-Hong , Xia Zhi-Kuan , Yang Xin , Luo Wan-Ting , Ao Jun-Hong , Yang Rong-Ya 
Published: June 2020

Abstract
Background: Trichosporon asahii (T. asahii) invasive infection frequently occurs in immunodeficient hosts with high mortality, but the pathogenesis of T. asahii infection remains elusive. Circular RNAs (circRNAs) are a type of endogenous noncoding RNAs that participant various disease processes. However, the mechanism of circRNAs in T. asahii infection are still completely unknown.Methods: RNA sequencing (RNA-seq) was performed to analyse the expression profiles of circRNA, microRNA (miRNA), and mRNA in THP-1 cells infected with T. asahii or uninfected samples. Part of the RNA-seq results were verified by RT-qPCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used to analyse the differentially expressed (DE) mRNA. The circRNA-miRNA-mRNA network was constructed and verified with dual-luciferase reporter assay and overexpression experiment.Results: A total of 46 circRNAs , 412 mRNAs and 47 miRNAs were DE after T. asahii infection at 12 h. GO and KEGG analysis showed that the DE mRNAs were primary linked to the leukocyte migration involved in inflammatory response, Toll-like receptor signaling pathway, and TNF signaling pathway. A competing endogenous RNA (ceRNA) network was constructed with five DE circRNAs, five DE miRNAs and 42 DE mRNAs. Among them, we verified that hsa_circ_0065336 indirectly regulate PTPN11 expression by sponging miR-505-3p.Conclusions: These data revealed a comprehensive circRNA-associated ceRNA network during T. asahii infection, thus providing new insights to clarify the pathogenesis between T. asahii-host interation.
Skewed expression profile of receptors for BAFF on peripheral blood B lymphocytes in Graves’ Disease
Authors: Wang Xin , Huang Jinhui , Zhang Aixia , Fang Chen , Ma Qi , Jiang Penjun 
Published: June 2020

Abstract
Background
Previous studies have demonstrated altered serum B lymphocyte activating factor (BAFF) level and expression of BAFF and BAFF receptor (BAFF-R) in thyroid infiltrating lymphocytes in Graves’ disease (GD) patients. The aim of the present study was to investigate expression of receptors for BAFF on the peripheral blood B lymphocytes in addition to serum BAFF concentrations.
Methods
Fifty-two GD patients and twenty-three healthy controls (HC) were recruited in this study. Serum BAFF concentrations were measure by ELISA. Expression of receptors for BAFF on peripheral blood B lymphocytes, including BAFF-R and transmembrane activator and calcium-modulating and cyclophilin ligand interactor (TACI), was analyzed by flow cytometry. Serum BAFF levels, expression of BAFF-R and TACI on peripheral blood B lymphocytes were compared between GD and HC groups. Correlations between serum BAFF concentrations and thyroid function and thyrotropin receptor autoantibody (TRAb) titers were analyzed in GD patients. In 10 patients with Graves’ orbitopathy (GO) receiving steroids therapy, effects of steroids on serum BAFF levels and expression of BAFF-R and TACI were observed.
Results
Serum BAFF levels were significantly higher in GD patients (1.18 ± 0.33 ng/ml) than those in HC (0.93 ± 0.24 ng/ml, P = 0.0011). In GD patients, serum BAFF levels correlated positively with free thyroxine concentrations (Spearman r = 0.2460, P = 0.0394), but not TRAb titers (P = 0.4734). BAFF-R expression on peripheral B lymphocytes were elevated in GD (mean MFI: 4.52 ± 2.06 in GD vs 3.00 ± 0.87 in HC, P < 0.0001), while TACI expression on peripheral B lymphocytes were decreased in GD (mean MFI: 7.96 ± 4.06 in GD vs 9.10 ± 3.37 in HC, P = 0.0331). Steroids significantly suppressed serum BAFF concentrations ( from 1.18 ± 0.27 ng/ml to 0.97 ± 0.10 ng/ml, P = 0.0364) and BAFF-R expression on peripheral blood B lymphocytes in GO patients (mean MFI from 6.26 ± 4.91 to 4.05 ± 1.58, P = 0.0083). Change of TACI expression on B lymphocytes was not statistically significant after steroids therapy.
Conclusions
Skewed expression profile of receptors for BAFF on B lymphocytes may mediate the autoimmunity in GD. Restoring the normal expression profile of receptors for BAFF could be a new strategy for treating and cueing GD.
The Effect of Root Native Tree Species on Soil Shear Strength on Hillslopes of Sierra Madre Oriental, Mexico
Authors: Zavala-González Rebeca Guadalupe , Cantú-Silva Israel , González-Rodríguez Humberto 
Published: June 2020

Abstract
Background: The presence of vegetation reduces soil erosion and shallow slope failure both by reinforcing soil shear resistance and influencing the geo-mechanic conditions of soil. For this reason, vegetation strategies in areas vulnerable to erosion are considered to be an effective control measure for soil erosion. Method: The tree species used in this research are widespread in the slopes of Chipinque mountain of Sierra Madre Oriental and belong to four native species: Cercis canadensis, Celtis laevigata, Quercus rysophylla and Ligustrum lucidum. In order to investigate the mechanical characteristics of roots, single roots specimens were sampled and tested for tensile strength. The tests were conducted with the Universal Testing Machine Shimadzu type SLFL-100KN to evaluate the influence of root shear strength on the soil using the Wu Model (Wu et al., 1979) as well as to analyze root cohesion and Root Area Ratio (RAR). The latter was calculated by taking both direct (field) and indirect measurements on image processing.Results: The results show that C. laevigata roots have the strongest tensile strenght, followed by Q. rysophylla > C. Canadensis > L. lucidum. RAR ranges from C. laevigata (0.0587%) > C. Canadensis (0.0585%) >L. lucidum (0.0504%) > Q. rysophylla (0.0441%). L. lucidum provides the less increase soil shear strength through root cohesion (16.12 kN/m2) > C. canadensis (53.70 kN/m2) > Q. rysophylla (89.07 kN/m2) to C. laevigata (97.41 kN/m2)
Comparative Evaluation of P16, Ki-67, and Shape-correlated Genes as Biomarkers for Cervical High-grade Squamous Intraepithelial Lesions
Authors: Sun hengzi , Li Shuhong , Cao Dongyan , Yang Jiaxin , Peng Peng , Kong Linghua , Chen Fei , Huo Xiao , Shen Keng 
Published: June 2020

Abstract
Background:Colposcopy was referred in cases with severe abnormalities in co-testing. However, approximately 60%-80% patients did not receive proper benefits. Although p16/Ki67 dual staining reduced the referral rate, its sensitivity and specificity need to be enhanced. Methods:The expression of p16, Ki-67, SMAD3, YAP1, RELA were evaluated inthe colposcopy referral population. Results:The sensitivity and specificity of p16+ combined with Ki-67+ for predicting CIN2+ were 62.1% and 89.5%, respectively. p16+ combined with YAP1+ and/or RELA+ provided a sensitivity and specificity of 70.9% and 89.5%, respectively, while 72.8% and 86.4% were achieved by p16+ combined with YAP1+ and/or SMAD3+ and/or RELA+. In HPV16/18+ and LSIL subgroups, the sensitivity and specificity of p16+ combined with Ki-67+ for predicting CIN2+ were 67.7% and 87.6%, respectively, for the former group and 58.6%, 88.8%, respectively, for the latter group, whereas those of RELA alone were 76.9% and 90.5%, respectively, and 82.8% and 92.1%, respectively. p16+, YAP1+/RELA+ showed a better performance for predicting CIN2+ with a better sensitivity (86.7% vs. 52.2%, p=0.028) and considerable specificity (86.7% vs. 90.0%, p=0.486) in the other HPV+ combined with ASCUS groupthan were achieved by p16+ combined with Ki-67+. RELA+ and the combination of p16 and RELA/YAP1 also provided the Max AUC area. Conclusions:Our study shows that RELA and the combination of p16 and RELA/YAP1 achieved better sensitivity and specificity for detecting morphologically CIN2+ lesions.
Ezh2 Inhibitors Reverse Resistance to Gefitinib in Primary Egfr Wild-type Lung Cancer Cells
Authors: Gong Hao , Li Yongwen , Yuan Yin , Li Weiting , Zhang Hongbing , Zhang Zihe , Shi Ruifeng , Liu Minghui , Liu Chao , Liu Hongyu , Chen Jun 
Published: June 2020

Abstract
Background: Lung cancer is the leading cause of cancer-related death worldwide. Non–small-cell lung cancer (NSCLC) is the most common type of lung cancer. Traditional anticancer therapies involving epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (EGFR-TKIs) have been proven beneficial in the treatment of patients with EGFR mutations. However, patients with EGFR wild-type NSCLC usually fail to respond to EGFR-TKIs. Enhancer of zeste homolog 2 (EZH2), a key molecule of the PRC2 complex, plays an important role in epigenetic regulation and is overexpressed in various tumors. EZH2 inhibitors sensitize various types of tumor cells to antitumor drugs. Therefore, this study aimed to investigate whether the EZH2 inhibitors GSK343 and DZNep, whencombined with gefitinib, can reverse EGFR-TKI resistance in EGFR wild-type NSCLC. Methods:EZH2 expression was evaluated using the RNA sequencing dataset of NSCLC patients (502 lung squamous cell carcinoma cases including 49 paracancerous lung tissues and 513 lung adenocarcinoma cases including 59 paracancerous lung tissues) from The Cancer Genome Atlas (TCGA). We simultaneously also verified EZH2 expressionin 40 NSCLC samples and their corresponding paracancerous lung tissues from our institution via quantitative PCR. The lung adenocarcinoma cell lines A549 and H1299 were treated with EZH2-specific small interfering RNA or EZH2 inhibitors and subjected to analyses of cell viability and apoptosis as well as of EGFR pathway protein expressions by western blotting. Results: EZH2 was upregulated in human NSCLC tissues and was correlated with poor prognosis in patients with lung adenocarcinoma based on data from both TCGA and our institution. Both EZH2 inhibitors sensitized A549 and H1299 cells to gefitinib and suppressed cell viability and proliferation in vitroby downregulating the phosphorylation of EGFR and AKT and inducing cell apoptosis. Co-administration of EZH2 inhibitors (GSK343 or DZNep) with gefitinib exerted stronger inhibitory effects on tumor activity, cell proliferation, and cell migration than single drug administration in vitro and in vivo.Conclusion: Co-administration of EZH2 inhibitors with EGFR-TKIs may be feasible for the treatment of EGFR wild-type NSCLC in patients who refuse traditional chemotherapy.
Prognostic role of sarcopenia and body composition with long-term outcomes in obstructive colorectal cancer
Authors: Lee Chul Seung , Won Daeyoun David , Oh Soon Nam , Lee Yoon Suk , Lee In Kyu , Kim In-Ho , Choi Moon Hyung , Oh Seong Taek 
Published: June 2020

Abstract
Background The clinical significance of sarcopenia in colorectal cancer obstruction has not yet been described. The present study aimed to determine the short and long-term oncologic impacts of sarcopenia in obstructive colorectal cancer. MethodsA total of 214 patients with obstructive colon cancer were included in this study, between January 2004 and December 2013. Initial staging computed tomography (CT) scans identified sarcopenia and visceral obesity by measuring the muscle and visceral fat areas at the third lumbar vertebra level. Both short-term postoperative and long-term oncologic outcomes were analyzed. Results Among all 214 patients, 71 (33.2%) were diagnosed with sarcopenia. Sarcopenia had a negative oncologic impact in both disease-free survival (DFS) and overall survival (OS), (hazard ratio [HR] = 1.860, 95% confidence interval [CI] 1.035 – 3.128, p = 0.037, and HR = 1.917, CI 1.021 – 3.602, p = 0.043, respectively). Visceral adiposity, body mass index (BMI) and neutrophil-lymphocyte ratio (NLR) did not significantly impact DFS and OS. Conclusion Sarcopenia is a clinical factor significantly associated with OS and DFS but not with short-term complications in obstructive colorectal cancer. In future, prospective studies should incorporate body composition data in patient risk assessments and oncologic prediction tools.
Pyrolysis and Combustion Characteristics of Newly Discovered Coals from Nigeria: Physicochemical, Microstructure, Mineralogy, and Thermal Properties
Authors: Nyakuma Bemgba Bevan , Jauro Aliyu , Akinyemi Segun Ajayi , Faizal Hasan Mohd , Nasirudeen Mohammed Baba , Fuad Muhammad Ariff Hanaffi Mohd , Oladokun Olagoke 
Published: June 2020

Abstract
Despite Nigeria’s vast mineral and energy resources, the nation lacks sufficient electricity generation capacity to stimulate sustainable growth and infrastructural development. However, the discovery of vast coal deposits across the nation’s sedimentary basins could provide cheap, reliable, and abundant energy supplies. However, the lack of comprehensive data on the fuel characteristics, energy recovery, and potential emissions of Nigeria’s coals currently hampers the quest for coal-fired electricity generation. Hence, this study examined the physicochemical, microstructural, mineralogical, and thermal fuel properties of three (3) newly discovered coals from Akunza (AKZ), Ome (OME), and Shiga (SHG) in Nigeria for potential energy recovery through combustion and pyrolysis. Physicochemical analysis revealed high combustible but low levels of polluting elements. The higher heating values ranged from 18.65 MJ/kg for AKZ to 26.59 MJ/kg for SHG. Microstructure analysis revealed rough textured and surfaced particles with a glassy lustre ascribed to metals (such as Ti and Fe), quartz, and kaolinite. Mineralogical analyses revealed the major elements (C, O, Si, and Al), along with minor (Ca, Cu, Fe, K, Mg, S, and Ti) associated with clays, salts, or the porphyrin constituents of coal. Thermal analysis showed mass losses (ML) ranging from 30.51% to 87.57% and residual mass (RM) from 12.44% to 69.49% under oxidative (combustion) and non-oxidative (pyrolysis) conditions due to the thermal degradation of organic matter and macerals (vitrinite, inertinite and liptinite) during TGA. Overall, the coals are potential feedstocks for energy recovery through pyrolysis and combustion.
Nomogram for the Prediction of Contrast-associated Acute Kidney Injury in Patients with Hypoalbuminemia Undergoing Coronary Angiography
Authors: Liu Liwei , Liu Jin , Lei Li , Wang Bo , Sun Guoli , Guo Zhaodong , He Yibo , Song Feier , Lun Zhubin , Liu Bowen , Chen Guanzhong , Chen Shiqun , Yang Yongquan , Chen Jiyan , Liu Yong 
Published: June 2020

Abstract
Background: Risk stratification is recommended as the key step to prevent contrast-associated acute kidney injury (CA-AKI) by allowing for prevention among at-risk patients undergoing coronary angiography (CAG) or percutaneous coronary intervention (PCI). Patients with hypoalbuminemia are prone to CA-AKI and do not have their own risk stratification tool. Therefore, we developed and validated a nomogram for predicting CA-AKI in patients with hypoalbuminemia undergoing CAG/PCI.Methods: A total of 1272 consecutive patients with hypoalbuminemia undergoing CAG/PCI were enrolled and randomly assigned (2:1 ratio) to a development cohort (n = 848) and a validation cohort (n = 424). CA-AKI was defined as a serum creatinine (SCr) increase of ≥ 0.3 mg/dL or 50% from baseline within the first 48 to 72 hours following CAG/PCI. A nomogram was established with independent predictors according to multivariate logistic regression and a stepwise approach. The discrimination of the nomogram was assessed by the area under the receiver operating characteristic (ROC) curve and was compared to the classic Mehran CA-AKI score. Calibration was assessed using the Hosmer–Lemeshow test.Results: Overall, 8.4% (71/848) of patients in the development cohort and 11.2% (48/424) of patients in the validation cohort experienced CA-AKI. The simple nomogram included estimated glomerular filtration rate (eGFR), serum albumin (ALB), age and the use of intra-aortic balloon pump (IABP); showed better predictive ability than the Mehran score (C-index 0.756 vs. 0.693, p = 0.02); and had good calibration (Hosmer–Lemeshow test p = 0.187). Decision curve analysis showed that the nomogram was more clinically useful than the Mehran score.Conclusions: Our data suggested that the simple nomogram might be a good tool for predicting CA-AKI in high-risk patients with hypoalbuminemia undergoing CAG/PCI, but our findings require further external validation.Trial registration number NCT01400295
Biomass Cellulose Nanoparticles Display Considerable Neurotoxicity in Zebrafish
Authors: Liu Cong , Zhao Jinxiang , Wei Guanyun , Hao Wenjin , Wang Xin , Yang Chao , Shi Yunwei , Liu Dong 
Published: June 2020

Abstract
BackgroundThe widespread use of nanomaterials poses a great threat to human living environments. Among them, biomass cellulose nanoparticle (CN) is one of the widely used nanomaterials. To date, the toxicity of CNs during embryonic development remains undetermined. In this study, we exposed zebrafish embryos to cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs) to evaluate the toxicity of these CNs. ResultsExposure to CNFs or CNCs below 30 mg/ml exhibited no dose-dependent increases in malformation and mortality in zebrafish embryos. Then we demonstrated that CNs were highly enriched in zebrafish embryo via imaging analysis of embryos treated with FITC-coupled CNCs. In addition, we found that CNF or CNC exposure resulted in compromised motor ability of zebrafish larva. Furthermore, it was revealed that the differentiation and the morphogenesis of motor neurons were significantly interrupted. While, blood vessels were normally patterned, suggesting the specific neurotoxicity of those materials. Transcriptome sequencing assay demonstrated that the neurotoxicity of CNs in the motor neurons might be attributed to the expression alteration of neural genes. ConclusionWe found that the specific neurotoxicity of CNF and CNC using zebrafish model. CNF or CNC exposure interrupted the morphogenesis of motor neurons, which resulted in the compromised motor ability of zebrafish larva. In addition, the neurotoxicity of CNs in the motor neurons might be attributed to the expression alteration of neural genes.