bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hot-water immersion does not increase postprandial muscle protein synthesis rates during recovery from resistance-type exercise in healthy, young males
Authors: Fuchs Cas J. , Smeets Joey S. J. , Senden Joan M. , Zorenc Antoine H. , Goessens Joy P. B. , van Marken Lichtenbelt Wouter D. , Verdijk Lex B. , van Loon Luc J. C. 
Published: March 2020

The purpose of this study was to assess the impact of postexercise hot-water immersion on postprandial myofibrillar protein synthesis rates during recovery from a single bout of resistance-type exercise in healthy, young men. Twelve healthy, adult men (age: 23 ± 1 y) performed a single bout of resistance-type exercise followed by 20 min of water immersion of both legs. One leg was immersed in hot water [46°C: hot-water immersion (HWI)], while the other leg was immersed in thermoneutral water (30°C: CON). After water immersion, a beverage was ingested containing 20 g intrinsically L-[1-13C]-phenylalanine and L-[1-13C]-leucine labeled milk protein with 45 g of carbohydrates. In addition, primed continuous L-[ ring-2H5]-phenylalanine and L-[1-13C]-leucine infusions were applied, with frequent collection of blood and muscle samples to assess myofibrillar protein synthesis rates in vivo over a 5-h recovery period. Muscle temperature immediately after water immersion was higher in the HWI compared with the CON leg (37.5 ± 0.1 vs. 35.2 ± 0.2°C; P < 0.001). Incorporation of dietary protein-derived L-[1-13C]-phenylalanine into myofibrillar protein did not differ between the HWI and CON leg during the 5-h recovery period (0.025 ± 0.003 vs. 0.024 ± 0.002 MPE; P = 0.953). Postexercise myofibrillar protein synthesis rates did not differ between the HWI and CON leg based upon L-[1-13C]-leucine (0.050 ± 0.005 vs. 0.049 ± 0.002%/h; P = 0.815) and L-[ ring-2H5]-phenylalanine (0.048 ± 0.002 vs. 0.047 ± 0.003%/h; P = 0.877), respectively. Hot-water immersion during recovery from resistance-type exercise does not increase the postprandial rise in myofibrillar protein synthesis rates. In addition, postexercise hot-water immersion does not increase the capacity of the muscle to incorporate dietary protein-derived amino acids in muscle tissue protein during subsequent recovery. NEW & NOTEWORTHY This is the first study to assess the effect of postexercise hot-water immersion on postprandial myofibrillar protein synthesis rates and the incorporation of dietary protein-derived amino acids into muscle protein. We observed that hot-water immersion during recovery from a single bout of resistance-type exercise does not further increase myofibrillar protein synthesis rates or augment the postprandial incorporation of dietary protein-derived amino acids in muscle throughout 5 h of postexercise recovery.
Countering disuse atrophy in older adults with low-volume leucine supplementation
Authors: Arentson-Lantz Emily J. , Fiebig Kinga N. , Anderson-Catania Kim J. , Deer Rachel R. , Wacher Adam , Fry Christopher S. , Lamon Séverine , Paddon-Jones Douglas 
Published: March 2020

Older adults are at increased risk of being bedridden and experiencing negative health outcomes including the loss of muscle tissue and functional capacity. We hypothesized that supplementing daily meals with a small quantity (3–4 g/meal) of leucine would partially preserve lean leg mass and function of older adults during bed rest. During a 7-day bed rest protocol, followed by 5 days of inpatient rehabilitation, healthy older men and women (67.8 ± 1.1 yr, 14 men; 6 women) were randomized to receive isoenergetic meals supplemented with leucine (LEU, 0.06 g/kg/meal; n = 10) or an alanine control (CON, 0.06 g/kg/meal; n = 10). Outcomes were assessed at baseline, following bed rest, and after rehabilitation. Body composition was measured by dual-energy X-ray absorptiometry. Functional capacity was assessed by knee extensor isokinetic and isometric dynamometry, peak aerobic capacity, and the short physical performance battery. Muscle fiber type, cross-sectional area, signaling protein expression levels, and single fiber characteristics were determined from biopsies of the vastus lateralis. Leucine supplementation reduced the loss of leg lean mass during bed rest (LEU vs. CON: −423 vs. −1035 ± 143 g; P = 0.008) but had limited impact on strength or endurance-based functional outcomes. Similarly, leucine had no effect on markers of anabolic signaling and protein degradation during bed rest or rehabilitation. In conclusion, providing older adults with supplemental leucine has minimal impact on total energy or protein consumption and has the potential to partially counter some, but not all, of the negative effects of inactivity on muscle health. NEW & NOTEWORTHY Skeletal muscle morphology and function in older adults was significantly compromised by 7 days of disuse. Leucine supplementation partially countered the loss of lean leg mass but did not preserve muscle function or positively impact changes at the muscle fiber level associated with bed rest or rehabilitation. Of note, our data support a relationship between myonuclear content and adaptations to muscle atrophy at the whole limb and single fiber level.
LncSpA: LncRNA Spatial Atlas of Expression across Normal and Cancer Tissues
Authors: Lv Dezhong , Xu Kang , Jin Xiyun , Li Junyi , Shi Yuchen , Zhang Mengying , Jin Xiaoyan , Li Yongsheng , Xu Juan , Li Xia 
Published: March 2020

Abstract

Long noncoding RNAs (lncRNA) play important roles in maintaining morphology and function of tissues, and their regulatory effectiveness is closely associated with spatial expression. To provide a comprehensive spatial atlas of expression for lncRNA, we propose LncSpA (http://bio-bigdata.hrbmu.edu.cn/LncSpA) to explore tissue-elevated (TE) lncRNA across human normal and adult and pediatric cancer tissues. In total, 71,131 and 12,007 TE lncRNAs and 634 clinical-related TE lncRNAs were identified across 38 normal and 33 adult cancer tissues. Moreover, 4,688 TE and 413 clinical-related lncRNAs were identified in pediatric cancer. By quick searching or query options, users can obtain eight major types of detailed information for lncRNA via various visualization techniques, including qualitative and quantitative spatial expression in different resources, coexpressed mRNAs, predicted function, known disease association, and the potential to serve as diagnostic or prognostic markers. LncSpA will be a valuable resource to understand lncRNA functions across tissues and cancers, leading to enhanced therapeutic strategies in precision oncology.

Significance:
LncSpA is a new interactive resource that provides the spatial expression pattern of lncRNA across thousands of normal and cancer samples representing major tissue types.
The Evolutionary Origins of Recurrent Pancreatic Cancer
Authors: Sakamoto Hitomi , Attiyeh Marc A. , Gerold Jeffrey M. , Makohon-Moore Alvin P. , Hayashi Akimasa , Hong Jungeui , Kappagantula Rajya , Zhang Lance , Melchor Jerry P. , Reiter Johannes G. , Heyde Alexander , Bielski Craig M. , Penson Alexander V. , Gönen Mithat , Chakravarty Debyani , O'Reilly Eileen M. , Wood Laura D. , Hruban Ralph H. , Nowak Martin A. , Socci Nicholas D. , Taylor Barry S. , Iacobuzio-Donahue Christine A. 
Published: March 2020

Abstract

Surgery is the only curative option for stage I/II pancreatic cancer; nonetheless, most patients will experience a recurrence after surgery and die of their disease. To identify novel opportunities for management of recurrent pancreatic cancer, we performed whole-exome or targeted sequencing of 10 resected primary cancers and matched intrapancreatic recurrences or distant metastases. We identified that recurrent disease after adjuvant or first-line platinum therapy corresponds to an increased mutational burden. Recurrent disease is enriched for genetic alterations predicted to activate MAPK/ERK and PI3K–AKT signaling and develops from a monophyletic or polyphyletic origin. Treatment-induced genetic bottlenecks lead to a modified genetic landscape and subclonal heterogeneity for driver gene alterations in part due to intermetastatic seeding. In 1 patient what was believed to be recurrent disease was an independent (second) primary tumor. These findings suggest routine post-treatment sampling may have value in the management of recurrent pancreatic cancer.

Significance:
The biological features or clinical vulnerabilities of recurrent pancreatic cancer after pancreaticoduodenectomy are unknown. Using whole-exome sequencing we find that recurrent disease has a distinct genomic landscape, intermetastatic genetic heterogeneity, diverse clonal origins, and higher mutational burden than found for treatment-naïve disease.
See related commentary by Bednar and Pasca di Magliano, p. 762.
This article is highlighted in the In This Issue feature, p. 747
Acetaminophen (Paracetamol) Metabolites Induce Vasodilation and Hypotension by Activating Kv7 Potassium Channels Directly and Indirectly
Authors: van der Horst Jennifer , Manville Rian W. , Hayes Katie , Thomsen Morten B. , Abbott Geoffrey W. , Jepps Thomas A. 
Published: March 2020

Objective:
Intravenous acetaminophen/paracetamol (APAP) is well documented to cause hypotension. Since the patients receiving intravenous APAP are usually critically ill, any severe hemodynamic changes, as with those associated with APAP, can be life-threatening. The mechanism underlying this dangerous iatrogenic effect of APAP was unknown.

Approach and Results:
Here, we show that intravenous APAP caused transient hypotension in rats, which was attenuated by the Kv7 channel blocker, linopirdine. APAP metabolite N-acetyl-p-benzoquinone imine caused vasodilatation of rat mesenteric arteries ex vivo. This vasodilatation was sensitive to linopirdine and also the calcitonin gene-related peptide antagonist, BIBN 4096. Further investigation revealed N-acetyl-p-benzoquinone imine stimulates calcitonin gene-related peptide release from perivascular nerves, causing a cAMP-dependent activation of Kv7 channels. We also show that N-acetyl-p-benzoquinone imine enhances Kv7.4 and Kv7.5 channels overexpressed in oocytes, suggesting that it can activate Kv7.4 and Kv7.5 channels directly, to elicit vasodilatation.

Conclusions:
Direct and indirect activation of Kv7 channels by the APAP metabolite N-acetyl-p-benzoquinone imine decreases arterial tone, which can lead to a drop in blood pressure. Our findings provide a molecular mechanism and potential preventive intervention for the clinical phenomenon of intravenous APAP-dependent transient hypotension.
A Role for MMP-10 (Matrix Metalloproteinase-10) in Calcific Aortic Valve Stenosis
Authors: Matilla Lara , Roncal Carmen , Ibarrola Jaime , Arrieta Vanessa , García-Peña Amaia , Fernández-Celis Amaya , Navarro Adela , Álvarez Virginia , Gainza Alicia , Orbe Josune , Cachofeiro Victoria , Zalba Guillermo , Sádaba Rafael , Rodríguez José A. , López-Andrés Natalia 
Published: March 2020

Objective:
Aortic valve (AV) calcification plays an important role in the progression of aortic stenosis (AS). MMP-10 (matrix metalloproteinase-10 or stromelysin-2) is involved in vascular calcification in atherosclerosis. We hypothesize that MMP-10 may play a pathophysiological role in calcific AS.

Approach and Results:

Blood samples (n=112 AS and n=349 controls) and AVs (n=88) from patients undergoing valve replacement were analyzed. Circulating MMP-10 was higher in patients with AS compared with controls (
P
<0.001) and correlated with TNFα (tumor necrosis factor α; r
S
=0.451;
P
<0.0001). MMP-10 was detected by immunochemistry in AVs from patients with AS colocalized with aortic valve interstitial cells markers α-SMA (α-smooth muscle actin) and vimentin and with calcification markers Runx2 (Runt-related transcription factor 2) and SRY (sex-determining region Y)-box 9. MMP-10 expression in AVs was further confirmed by RT-qPCR and western blot. Ex vivo, MMP-10 was elevated in the conditioned media of AVs from patients with AS and associated with interleukin-1β (r
S
=0.5045,
P
<0.001) and BMP (bone morphogenetic protein)-2 (r
S
=0.5003,
P
<0.01). In vitro, recombinant human MMP-10 induced the overexpression of inflammatory, fibrotic, and osteogenic markers (interleukin-1β, α-SMA, vimentin, collagen, BMP-4, Sox9, OPN [osteopontin], BMP-9, and Smad 1/5/8;
P
<0.05) and cell mineralization in aortic valve interstitial cells isolated from human AVs, in a mechanism involving Akt (protein kinase B) phosphorylation. These effects were prevented by TIMP-1 (tissue inhibitor of metalloproteinases type 1), a physiological MMP inhibitor, or specifically by an anti-MMP-10 antibody.

Conclusions:
MMP-10, which is overexpressed in aortic valve from patients with AS, seems to play a central role in calcification in AS through Akt phosphorylation. MMP-10 could be a new therapeutic target for delaying the progression of aortic valve calcification in AS.
Induction of heat shock protein 70 gene expression in early life stages of the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879) (Decapoda, Palaemonidae)
Authors: Polsuwan Aurawan , Damrongphol Praneet 
Published: March 2020

Abstract
Heat shock proteins play an important role in maintaining normal cell function and overcoming stress conditions in organisms. Expression of the heat shock protein 70 gene (hsp70) at different stages of embryos and larvae of the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879) was analysed using quantitative real-time polymerase chain reaction. Expression of hsp70 was first detected in embryos of 5.5 days old; the expression levels gradually increased during the course of embryonic development, reaching their maximum at the stage prior to hatching. Whereas the expression levels were high in newly hatched larvae, they lowered subsequently before increasing to their maximum after 20 days post-hatch. Moreover, induction of hsp70 expression in 5.5 days old embryos and newly hatched larvae by two xenobiotics, 4-nonylphenol (4-NP) and chlorpyrifos (CPF) was also investigated. The results showed that both 4-NP and CPF caused similar patterns of hsp70 expression changes. Embryos exposed to 4-NP or CPF for 24 h increased the hsp70 expression levels with increasing concentrations of 4-NP or CPF. Prolonged exposure for 96 h induced higher levels of responses. The larvae were more sensitive than the embryos to both 4-NP and CPF challenges, by responding to lower concentrations of stressors and producing higher expression levels. The study suggests that hsp70 plays an active role during early life stages of M. rosenbergii, indicating the presence of mechanisms to overcome stress caused by development or by external stressors at these stages.
Northernmost record of the rare brachyuran crab, Paragalene longicrura (Nardo, 1868) (Decapoda, Progeryonidae) from the Istrian Peninsula (Adriatic Sea, Croatia)
Authors: Iveša Neven , Béres Tibor , Török Júlia K. 
Published: March 2020

Abstract
Between 2014 and 2019, four specimens of Paragalene longicrura (Nardo, 1868) were accidentally caught in the Adriatic Sea near Pula (Istrian Peninsula, Croatia). These are the first records of the little known species from the northern Adriatic Sea, as well as the northernmost occurrence over its global distribution. We have collected all documented occurrences from the literature since the description of the crab, resulting in a total number of 85 known specimens. Upon comparing the habitat type and depth distribution of our specimens with the recorded sightings, water temperature, and not depth, appeared to be the major determining factor for vertical distribution. Twenty-nine specimens have been detected in museum inventories, but not included in published articles. Our discoveries increased the number of known Adriatic specimens from 7 to 11. For the first time, we compiled all available carapace measurements to get an impression of the size range of the species.
New records of two spider crabs (Decapoda, Brachyura, Majidae) from Korea
Authors: Lee Sang-Hui , Lee Sang-Kyu , Kim Jung Nyun , Kim Won 
Published: March 2020

Abstract
Two species of the family Majidae, Sakaija japonica (Rathbun, 1932) and Schizophroida simodaensis Sakai, 1933, are herein newly reported from Korean waters. These are also the first records of Sakaija Ng & Richer de Forges, 2015 and Schizophroida Griffin & Tranter, 1986 in Korea. There are currently 14 majoid species from nine genera in Korea. Herein, diagnoses of Sakaija japonica and Schizophroida simodaensis are provided together with illustrations.
Origen and “Angelomorphic Pneumatology”
Authors: Lee Justin J. 
Published: March 2020

Abstract
Recent years have seen a growing interest in Patristic “angelomorphic pneumatology”, a phrase used mostly to describe early pre-Nicene portrayals of the Holy Spirit. While not denying the existence of such pneumatologies or their shared theological character as observed by scholars of angelomorphic pneumatology, this article seeks to challenge the appropriateness of term “angelomorphic” in a pneumatological context, particularly against the backdrop of its original Christological usage. This study takes as an example Origen of Alexandria, whose pneumatology is not considered “angelomorphic” by the standards of current definitions, but contains certain undeniable features of this angelomorphic theological tradition.
Latin Emperors and Serbian Queens: Anna and Helena
Authors: Van Tricht Filip 
Published: March 2020

Abstract
Latin Constantinople in the 1240s and 1250s has often been considered a ripe fruit waiting to fall into the hands of one of the competing political entities in the region (Nicaea, Epiros, Bulgaria, etc.). This paper argues, on the contrary, that under Emperor Baldwin II (1240-1273) the Latin Empire remained a dynamic power in the post-1204 Byzantine world. The basis for this re-evaluation is a revisionist study of the genealogical relations between a number of leading families in the region (among others the Villehardouin, Da Verona, and Cayeux), creating networks both within Latin Romania and beyond. One of the main hypotheses advanced is that two Serbian queens – Stefan Nemanja’s third wife Anna (of Hainaut) and Stefan Uroš I’s wife Helena (Angelos/Courtenay) – were in fact what one might call Latin imperial princesses. This in turn leads to a reconsideration of Latin-Serbian relations in the period 1204-1261.
Enhanced pre-ictal cortical responsivity in migraine patients assessed by visual chirp stimulation
Authors: Perenboom Matthijs JL , van de Ruit Mark , Zielman Ronald , van den Maagdenberg Arn MJM , Ferrari Michel D , Carpay Johannes A , Tolner Else A 
Published: March 2020

Background Migraine is associated with altered sensory processing and cortical responsivity that may contribute to susceptibility to attacks by changing brain network excitability dynamics. To gain better insight into cortical responsivity changes in migraine we subjected patients to a short series of light inputs over a broad frequency range (“chirp” stimulation), designed to uncover dynamic features of visual cortex responsivity. Methods EEG responses to visual chirp stimulation (10–40 Hz) were measured in controls (n = 24) and patients with migraine with aura (n = 19) or migraine without aura (n = 20). Average EEG responses were assessed at (i) all EEG frequencies between 5 and 125 Hz, (ii) stimulation frequencies, and (iii) harmonic frequencies. We compared average responses in a low (10–18 Hz), medium (19–26 Hz) and high (27–40 Hz) frequency band. Results Responses to chirp stimulation were similar in controls and migraine subtypes. Eight measurements (n = 3 migraine with aura; n = 5 without aura) were assigned as “pre-ictal”, based on reported headache within 48 hours after investigation. Pre-ictally, an increased harmonic response to 22–32 Hz stimulation (beta band) was observed ( p = 0.001), compared to interictal state measurements. Conclusions We found chirp responses to be enhanced in the 48 hours prior to migraine headache onset. Visual chirp stimulation proved a simple and reliable technique with potential to detect changes in cortical responsivity associated with the onset of migraine attacks.
Superior Capsule Reconstruction for Irreparable Massive Rotator Cuff Tears: Does It Make Sense? A Systematic Review of Early Clinical Evidence
Authors: Altintas Burak , Scheidt Michael , Kremser Victor , Boykin Robert , Bhatia Sanjeev , Sajadi Kaveh R. , Mair Scott , Millett Peter J. 
Published: March 2020

Background: Treatment of irreparable massive rotator cuff tears (MRCTs) in patients without advanced glenohumeral osteoarthritis remains a challenge. Arthroscopic superior capsule reconstruction (SCR) represents a newer method for treatment with increasing popularity and acceptance. Purpose: To analyze the clinical evidence surrounding SCR and determine the current clinical outcomes postoperatively. Study Design: Systematic review. Methods: A systematic review of the literature was performed following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. Electronic databases of PubMed, MEDLINE, Cochrane, and Google Scholar were used for the literature search. The study quality was evaluated according to the Modified Coleman Methodology Score. Studies in English evaluating SCR outcomes were included. Results: Seven studies were reviewed, including 352 patients (358 shoulders) treated with arthroscopic SCR with the mean duration of follow-up ranging from 15 to 48 months (range, 12-88 months). Fourteen patients were lost to follow-up, leaving 338 patients (344 shoulders) with clinical outcome data. Graft types included dermal allografts (n = 3 studies), fascia lata autografts (n = 3), or both (n = 1). Most commonly, a double-row technique was utilized for humeral graft fixation. The most common complication included graft tears in 13% of patients, resulting in 15 SCR revisions and 7 reverse shoulder arthroplasties. Postoperatively, improvements in visual analog scale (2.5 to 5.9), American Shoulder and Elbow Surgeons (20 to 56), Japanese Orthopaedic Association (38.0), Subjective Shoulder Value (37.0 to 41.3), and Constant (11.6 to 47.4) scores were observed. Three studies reported respective satisfaction rates of 72.9%, 85.7% and 90%. Increases in external rotation, internal rotation, and abduction with improved strength in external rotation were observed postoperatively. Improvement of pseudoparalysis was also observed in 3 studies. One study reported return to sports in 100% of patients (2 competitively, 24 recreationally) with no adverse outcomes. Conclusion: SCR showed good to excellent short-term clinical outcomes with adequate pain relief and functional improvement. The current evidence suggests that the procedure is an alternative for symptomatic patients with irreparable MRCT; however, the included studies were fair to poor in quality, and there were some notable complications. Long-term follow-up will determine the longevity and ultimate role of this new method in the treatment of irreparable MRCT.