bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA-seq reveals novel mechanistic targets of withaferin A in prostate cancer cells
Authors: Kim Su-Hyeong , Hahm Eun-Ryeong , Singh Krishna B , Shiva Sruti , Stewart-Ornstein Jacob , Singh Shivendra V 
Published: January 2020

Abstract
Withaferin A (WA) is a promising phytochemical exhibiting in vitro and in vivo anticancer activities against prostate and other cancers, but the mechanism of its action is not fully understood. In this study, we performed RNA-seq analysis using 22Rv1 human prostate cancer cell line to identify mechanistic targets of WA. Kyoto Encyclopedia of Genes and Genomes pathway analysis of the differentially expressed genes showed most significant enrichment of genes associated with metabolism. These results were validated using LNCaP and 22Rv1 human prostate cancer cells and Hi-Myc transgenic mice as models. The intracellular levels of acetyl-CoA, total free fatty acids and neutral lipids were decreased significantly following WA treatment in both cells, which was accompanied by downregulation of mRNA (confirmed by quantitative reverse transcription-polymerase chain reaction) and protein levels of key fatty acid synthesis enzymes, including ATP citrate lyase, acetyl-CoA carboxylase 1, fatty acid synthase and carnitine palmitoyltransferase 1A. Ectopic expression of c-Myc, but not constitutively active Akt, conferred a marked protection against WA-mediated suppression of acetyl-CoA carboxylase 1 and fatty acid synthase protein expression, and clonogenic cell survival. WA was a superior inhibitor of cell proliferation and fatty acid synthesis in comparison with known modulators of fatty acid metabolism including cerulenin and etomoxir. Intraperitoneal WA administration to Hi-Myc transgenic mice (0.1 mg/mouse, three times/week for 5 weeks) also resulted in a significant decrease in circulating levels of total free fatty acids and phospholipids, and expression of ATP citrate lyase, acetyl-CoA carboxylase 1, fatty acid synthase and carnitine palmitoyltransferase 1A proteins in the prostate in vivo.
The difference between delivered and prescribed dialysate sodium in haemodialysis machines
Authors: Shendi Ali M , Davenport Andrew 
Published: January 2020

Abstract

Background
The choice of dialysate sodium (DNa) for haemodialysis (HD) patients remains controversial, with some studies reporting that a lower DNa improves blood pressure control and reduces intradialytic weight gain. Studies on DNa depend on the alignment of programmed to delivered DNa. We wished to determine whether there were differences between programmed and delivered DNa.

Methods
Dialysate samples were obtained from three dialysis machines: Fresenius 4008H (F4008H) and 5008S (F5008S) and B-Braun hemodiafiltration (HDF) Dialog+(BB). DNa was measured by indirect ion-selective electrode (ISE), flame photometry (FP) and ion chromatography (IC) at different DNa concentrations.

Results
We tested 18 F5008S, 18 F4008H and 31 BB machines over 153 HD treatments. The median measured minus programmed DNa was significantly greater with the BB machine [ISE, 7 (6–8); FP, 7 (6–8); IC, 6 (5–7)], followed by the F4008H [ISE, 5.5 (5–7); FP, 4 (2.25–5.75); IC, 4 (2–5)]and F5008S [ISE, 4 (2–5); FP, 1 (−1–1.75); IC, 1 (−0.5 to 2)] mEq/L (P &lt; 0.05). At higher programmed DNa (140–145 mEq/L), measured DNa was greater for the BB and F4008 machines by all methods (P &lt; 0.05), but only by ISE for the F5008 (P &lt; 0.05).

Conclusions
We noted a systematic bias in DNa delivery with measured DNa being greater than that programmed by our HD machines. The magnitude of the bias varied between machines and with DNa. Our results may help explain the diverse results reported in studies of DNa.
Evaluating Native Bee Communities and Nutrition in Managed Grasslands
Authors: Stein D S , Debinski D M , Pleasants J M , Toth A L 
Published: January 2020

Abstract
Native pollinators are important for providing vital services in agroecosystems; however, their numbers are declining globally. Bees are the most efficient and diverse members of the pollinator community; therefore, it is imperative that management strategies be implemented that positively affect bee community composition and health. Here, we test responses of the bee and flowering plant communities to land management treatments in the context of grasslands in the upper Midwestern United States, a critical area with respect to bee declines. Twelve sites were selected to examine floral resources and wild bee communities based on three different types of grasslands: tallgrass prairie remnants, ungrazed restorations, and grazed restorations. Total bee abundance was significantly higher in ungrazed restorations than remnants, but there were no significant differences among grasslands in community composition or Shannon diversity. Across the three grassland types we also examined mass and lipid stores as nutritional health indicators in three sweat bees (Halictidae), Augochlora pura, Agapostemon virescens, and Halictus ligatus. Although there were no differences in lipid content, total average bee mass was significantly higher in Ag. virescens collected from ungrazed restorations as compared to remnants. Floral abundance of native and non-native species combined was significantly higher in grazed restorations compared to remnants and ungrazed restorations. However, ungrazed restorations had higher abundance and richness of native flowering ramets. These data suggest that bee abundance and nutrition are driven by high abundance of native flowering plant species, rather than total flowering plants.
‘Marry in Haste …’: The (Partial) Abolition of Same-sex Marriage in Bermuda
Authors: Barker Nicola 
Published: January 2020

Abstract
In 2018, the British Overseas Territory of Bermuda revoked the right to marry for same-sex couples. In a judgment that reconceives the relationship between sexual orientation and religious freedoms, the Bermuda Supreme Court and Court of Appeal found this revocation to be unconstitutional. I explore the political and legal context in which same-sex marriage was granted and then revoked in Bermuda. I also consider the Bermuda Courts’ judgments in light of the subsequent judgment of the United Kingdom’s Supreme Court in Steinfeld, among others. While there was an assumption from both the Bermuda and United Kingdom Governments that the revocation provision was compatible with the European Convention on Human Rights, I argue that this underestimates the significance of the distinction between declining to recognise a right to same-sex marriage and revoking a right that has already been exercised. While the European Court of Human Rights has not yet found the absence of same-sex marriage to be a violation of Article 12 of the Convention, I argue that the revocation of a right to marry between same-sex couples that had been recognised in accordance with national law changes the terrain on which the Convention arguments would be made.
Year-long effects of high pCO2 on the community structure of a tropical fore reef assembled in outdoor flumes
Authors: Edmunds Peter J , Doo Steve S , Carpenter Robert C 
Published: January 2020

Abstract
In this study, fore reef coral communities were exposed to high pCO2 for a year to explore the relationship between net accretion (Gnet) and community structure (planar area growth). Coral reef communities simulating the fore reef at 17-m depth on Mo’orea, French Polynesia, were assembled in three outdoor flumes (each 500 l) that were maintained at ambient (396 µatm), 782 µatm, and 1434 µatm pCO2, supplied with seawater at 300 l h−1, and exposed to light simulating 17-m depth. The communities were constructed using corals from the fore reef, and the responses of massive Porites spp., Acropora spp., and Pocillopora verrucosa were assessed through monthly measurements of Gnet and planar area. High pCO2 depressed Gnet but did not affect colony area by taxon, although the areas of Acropora spp. and P. verrucosa summed to cause multivariate community structure to differ among treatments. These results suggest that skeletal plasticity modulates the effects of reduced Gnet at high pCO2 on planar growth, at least over a year. The low sensitivity of the planar growth of fore reef corals to the effects of ocean acidification (OA) on net calcification supports the counterintuitive conclusion that coral community structure may not be strongly affected by OA.
On the empirical performance of some new neural network methods for forecasting intermittent demand
Authors: Babai M Z , Tsadiras A , Papadopoulos C 
Published: January 2020

Abstract
In this paper, new neural network (NN) methods are proposed to forecast intermittent demand and we empirically study their performance as compared to parametric and non-parametric forecasting methods proposed in the literature. The empirical investigation uses demand data for 5,135 spare parts for the fleet of aircrafts of an airline company. Three parametric benchmark methods are examined: single exponential smoothing (SES), Croston’s method and Syntetos–Boylan approximation, along with two bootstrapping methods: Willemain’s method and Zhou and Viswanathan’s method. The benchmark NN method considered in this paper is that proposed by Gutierrez et al. (2008) The paper shows the outperformance of SES and the NN methods for (a) their forecast accuracy and (b) their inventory efficiency (trade-off between holding volumes and backordering volumes) when compared to the other methods. Moreover, among the NN methods, a new proposed method is shown to be better than that proposed by Gutierrez et al. in terms of forecast accuracy and inventory efficiency.
Behavioral and Endocrine Alterations to Partner Interactions and Offspring Care during Periods of Conflict
Authors: Paciorek Timothy , Joseph Leese 
Published: January 2020

Abstract
Biparental care has evolved to ensure successful rearing of offspring. However, separation during periods of care can lead to conflicts that might negatively impact pair bonds and offspring care. In this study, pair-bonded convict cichlids (Amatitlania nigrofasciata) were observed for changes in behavior toward their partners and offspring before and after a period of separation. Males and females were designated either as a Resident (remain with offspring) or Removed (separated from partner and offspring for 5 days) individual. Behaviors between partners and toward offspring were measured before and after separation, and compared to the levels of behavior of control pairs (never separated), as well as individuals introduced to a novel partner instead. Cortisol levels of Resident male and female A. nigrofasciata were assayed using water-borne hormone collection before and after separation. Aggression between pair bond members did increase following reintroduction, but did not lead to the termination of pair bonds. Resident females showed more aggression to novel partners than Resident males. Offspring care decreased in both Resident and Removed females. Experimental pairs decreased the amount of time spent interacting with intruders. Cortisol levels were significantly higher among experimental pairs compared with control pairs that did not experience a separation. Females (both control and experimental) showed small, yet significant increases in cortisol levels, while both control and experimental males did not. These results suggest that while pair bonds appear resilient, prolonged separations influence pair bond and parental care dynamics, both behaviorally and hormonally, and require pairs to re-establish roles, resulting in less time caring for offspring.
Simulation-driven product development of cast components with allowance for process-induced material behaviour
Authors: Jansson J , Olofsson J , Salomonsson K 
Published: January 2020

Abstract
This paper presents a methodology that can be used to consider local variations in thermomechanical and thermophysical material properties, residual stresses, and strength-differential effects in finite element analyses of cast components. The methodology is based on applying process simulations and structural analyses together with experimentally established, or already available literature data, in order to describe element-specific material variations. A cast-iron cylinder head was used in order to evaluate the influence of several simplifications that are commonly performed in computer aided engineering. It is shown that non-trivial errors of a potentially large magnitude are introduced by not considering residual stresses, compressive behaviour, temperature dependence, and process-induced material property variations. By providing design engineers with tools that allow them to consider the complex relationships between these aspects early in the development phase, cast components have the potential to be further optimized with respect to both weight and performance.
The malignancy of liver cancer cells is increased by IL-4/ERK/AKT signaling axis activity triggered by irradiated endothelial cells
Authors: Kim Sung Dae , Baik Ji Sue , Lee Jae-Hye , Mun Seo-Won , Yi Joo Mi , Park Moon-Taek 
Published: January 2020

Abstract
The malignant traits involved in tumor relapse, metastasis and the expansion of cancer stem-like cells are acquired via the epithelial–mesenchymal transition (EMT) process in the tumor microenvironment. In addition, the tumor microenvironment strongly supports the survival and growth of malignant tumor cells and further contributes to the reduced efficacy of anticancer therapy. Ionizing radiation can influence the tumor microenvironment, because it alters the biological functions of endothelial cells composing tumor vascular systems. However, to date, studies on the pivotal role of these endothelial cells in mediating the malignancy of cancer cells in the irradiated tumor microenvironment are rare. We previously evaluated the effects of irradiated endothelial cells on the malignant traits of human liver cancer cells and reported that endothelial cells irradiated with 2 Gy reinforce the malignant properties of these cancer cells. In this study, we investigated the signaling mechanisms underlying these events. We revealed that the increased expression level of IL-4 in endothelial cells irradiated with 2 Gy eventually led to enhanced migration and invasion of cancer cells and further expansion of cancer stem-like cells. In addition, this increased level of IL-4 activated the ERK and AKT signaling pathways to reinforce these events in cancer cells. Taken together, our data indicate that ionizing radiation may indirectly modulate malignancy by affecting endothelial cells in the tumor microenvironment. Importantly, these indirect effects on malignancy are thought to offer valuable clues or targets for overcoming the tumor recurrence after radiotherapy.
The art of nerves: A quantitative and qualitative analysis of drama at the turn of nineteenth and twentieth century
Authors: Karlińska Agnieszka 
Published: January 2020

AbstractThe aim of this article was to investigate the influence of medical discourse of the turn of the nineteenth and the twentieth centuries through a case study of dramatic works of the Polish modernism. It was based on a detailed analysis of ninety plays randomly selected out of all performances staged between 1890 and 1913 at two major theatres from Krakow and Warsaw. The author analysed the ways of dramatizing symptoms of hysteria and compared the language used in describing disorders in female and male characters, combining quantitative and qualitative methods of text analysis. Almost all the plays included the symptoms of hysteria; most of them presented characters prone to hysteria. The number of symptoms and hysterical characters increased in periods when new medical theories had gained popularity. The image of hysteria that emerges from the surveyed works both defied the stereotype of hysteria as a specifically feminine illness and confirmed these stereotypes. Dramatic works could have played an important role in the process of popularizing hysteria.
Type theoretical databases
Authors: Forssell Henrik , Robbestad Gylterud Håkon , Spivak David I 
Published: January 2020

Abstract
We show how the display-map category of finite (symmetric) simplicial complexes can be seen as representing the totality of database schemas and instances in a single mathematical structure. We give a sound interpretation of a certain dependent type theory in this model and show how it allows for the syntactic specification of schemas and instances and the manipulation of the same with the usual type-theoretic operations.
Effects of Brief Depression and Anxiety Management Training on a US Army Division’s Primary Care Providers
Authors: Amin Rohul , Thomas Mary Ann 
Published: January 2020

Abstract

Introduction
There is a nation-wide gap between the prevalence of mental illness and the availability of psychiatrists. This places reliance on primary care providers (PCPs) to help meet some of these mental health needs. Similarly, the US Army expects its PCPs to be able to manage common mental illnesses such as anxiety and depression. Therefore, PCPs must be able to close their psychiatric skills gaps via lifelong learning.

Materials and Methods
Following needs assessment of PCPs in a US Army division, the curriculum was developed. Objectives targeted pharmacological management of depression and anxiety. Behavioral intervention skills were also taught to treat insomnia. Didactics and case-based small groups were used. A novel psychotropic decisional tool was developed and provided to learners to assist and influence their future psychiatric practice. Pre-training, immediate post-training, and 6-month assessments were done via survey to evaluate confidence and perceived changes in practice. The curriculum was executed as a quality improvement project using the Plan, Do, Study, Act framework.

Results
Among 35 learners, immediate confidence in selecting optimal psychotropic and perceived knowledge, skill to change the dose or type of medication, and confidence in prescribing behavioral sleep improved significantly with large effect sizes. At 6-month follow-up, learners reported that they were more likely to adjust medications for anxiety or depression and were more likely to start a new medication for anxiety or depression because of the training with moderate effect sizes. Use and satisfaction with the psychotropic decisional tool are also reported.

Conclusions
Our psychotropic decisional tool illustrates a novel algorithmic approach for operationalizing the management of depression and anxiety. Similar approaches can improve the skills of a variety of PCPs in the management of psychiatric disorders. Further studies in the military operational setting are needed to assess the effects of similar educational interventions on access to behavioral health care, suicidal behaviors, and unit medical readiness.
Angle-action variables for orbits trapped at a Lindblad resonance
Authors: Binney James 
Published: January 2020

ABSTRACT
The conventional approach to orbit trapping at Lindblad resonances via a pendulum equation fails when the parent of the trapped orbits is too circular. The problem is explained and resolved in the context of the Torus Mapper and a realistic Galaxy model. Tori are computed for orbits trapped at both the inner and outer Lindblad resonances of our Galaxy. At the outer Lindblad resonance, orbits are quasi-periodic and can be accurately fitted by torus mapping. At the inner Lindblad resonance, orbits are significantly chaotic although far from ergodic, and each orbit explores a small range of tori obtained by torus mapping.
Real galaxy mergers from galaxy pair catalogues
Authors: Pfister Hugo , Dotti Massimo , Laigle Clotilde , Dubois Yohan , Volonteri Marta 
Published: January 2020

ABSTRACT
Mergers of galaxies are extremely violent events shaping their evolution. Such events are thought to trigger starbursts and, possibly, black hole accretion. Nonetheless, it is still not clear how to know the fate of a galaxy pair from the data available at a given time, limiting our ability to constrain the exact role of mergers. In this paper we use the light-cone of the horizon-agn simulation, for which we know the fate of each pair, to test three selection processes aiming at identifying true merging pairs. We find that the simplest one (selecting objects within two thresholds on projected distance d and redshift difference Δz) gives similar results than the most complex one (based on a neural network analysing d, Δz, redshift of the primary, masses/star formation rates/aspect ratio of both galaxies). Our best thresholds are $d_\mathrm{th}\sim 100\, \mathrm{kpc}$ and Δzth ∼ 10−3, in agreement with recent results.
Detection of Fe i in the atmosphere of the ultra-hot Jupiter WASP-121b, and a new likelihood-based approach for Doppler-resolved spectroscopy
Authors: Gibson Neale P , Merritt Stephanie , Nugroho Stevanus K , Cubillos Patricio E , de Mooij Ernst J W , Mikal-Evans Thomas , Fossati Luca , Lothringer Joshua , Nikolov Nikolay , Sing David K , Spake Jessica J , Watson Chris A , Wilson Jamie 
Published: January 2020

ABSTRACT
High-resolution Doppler-resolved spectroscopy has opened up a new window into the atmospheres of both transiting and non-transiting exoplanets. Here, we present VLT/UVES observations of a transit of WASP-121b, an ‘ultra-hot’ Jupiter previously found to exhibit a temperature inversion and detections of multiple species at optical wavelengths. We present initial results using the blue arm of UVES (≈3700–5000 Å), recovering a clear signal of neutral Fe in the planet’s atmosphere at &gt;8$\, \sigma$, which could contribute to (or even fully explain) the temperature inversion in the stratosphere. However, using standard cross-correlation methods, it is difficult to extract physical parameters such as temperature and abundances. Recent pioneering efforts have sought to develop likelihood ‘mappings’ that can be used to directly fit models to high-resolution data sets. We introduce a new framework that directly computes the likelihood of the model fit to the data, and can be used to explore the posterior distribution of parametrised model atmospheres via MCMC techniques. Our method also recovers the physical extent of the atmosphere, as well as account for time- and wavelength-dependent uncertainties. We measure a temperature of $3710^{+490}_{-510}$ K, indicating a higher temperature in the upper atmosphere when compared to low-resolution observations. We also show that the Fe i signal is physically separated from the exospheric Fe ii. However, the temperature measurements are highly degenerate with aerosol properties; detection of additional species, using more sophisticated atmospheric models, or combining these methods with low-resolution spectra should help break these degeneracies.
Reverse engineering the Milky Way
Authors: Forbes Duncan A 
Published: January 2020

ABSTRACT
The ages, metallicities, alpha-elements, and integrals of motion of globular clusters (GCs) accreted by the Milky Way from disrupted satellites remain largely unchanged over time. Here we have used these conserved properties in combination to assign 76 GCs to five progenitor satellite galaxies – one of which we dub the Koala dwarf galaxy. We fit a leaky-box chemical enrichment model to the age–metallicity distribution of GCs, deriving the effective yield and the formation epoch of each satellite. Based on scaling relations of GC counts we estimate the original halo mass, stellar mass, and mean metallicity of each satellite. The total stellar mass of the five accreted satellites contributed around 109 M⊙ in stars to the growth of the Milky Way but over 50 per cent of the Milky Way’s GC system. The five satellites formed at very early times and were likely accreted 8–11 Gyr ago, indicating rapid growth for the Milky Way in its early evolution. We suggest that at least three satellites were originally nucleated, with the remnant nucleus now a GC of the Milky Way. 11 GCs are also identified as having formed ex situ but could not be assigned to a single progenitor satellite.