bluuebunny/crossref_metadata_2025_split huggingface dataset:
Active Behavior of Triple-Negative Breast Cancer With Adipose Tissue Invasion: A Single Center and Retrospective Review
Authors: yamaguchi junzo , Moriuchi Hiroki , Ueda Takashi , Kawashita Yujo , Hazeyama Takanori , Tateishi Masaki , Aoki Shigehisa , Uchihashi Kazuyoshi , Nakamura Mikio 
Published: January 2021

Abstract
BackgroundInteractions between adipocyte and breast cancer (BC) cells have yet to be fully elucidated. Here we investigated the prognostic impact of marginal adipose tissue invasion in both luminal breast cancer (HR+/HER2-) and triple-negative breast cancer (TNBC) (HR-/HER2-). MethodsA total of 735 patients with early-stage invasive BC (1999-2014) were retrospectively registered. Median length of patient follow-up was 8.9 years. Survival curves were calculated using a Kaplan-Meier cumulative survival plot. The prognostic difference between two groups were assessed by the univariate Cox-proportional hazard regression model.ResultsPatients with adipose tissue invasion (n = 614) had a significantly poorer prognosis than those without adipose tissue invasion (n = 121) in overall survival (OS) (hazard ratio, 2.1; 95% Confidence interval [CI], 1.1 to 4.0; P = 0.025). While a poorer prognosis was observed in TNBC (n = 137) than in luminal BC patients (n = 496) (hazard ratio, 0.45; 95% CI, 0.30 to 0.68, P < 0.001), this aggressive nature of TNBC was noted in node-positive disease (hazard ratio, 0.3; 95% CI, 0.18 to 0.5, P < 0.001) but not in node-negative disease (hazard ratio, 0.78; 95% CI, 0.39 to 1.55, P = 0.472), and also noted in adipose tissue invasion-positive patients (hazard ratio, 0.4; 95% CI, 0.26 to 0.6, P < 0.001) but not in adipose tissue invasion-negative patients (hazard ratio, 0.73; 95% CI, 0.16 to 3.24, P = 0.675). In addition, although patients suffering from TNBC with adipose tissue invasion had a poorer outcome than those without adipose tissue invasion (hazard ratio, 3.63; 95% CI, 1.11 to 11.84; P = 0.033), the difference was not observed in luminal BC (hazard ratio, 1.75; 95% CI, 0.64 to 4.82; P = 0.277). ConclusionsAdipose tissue invasion was correlated with poor survival in TNBC. Cancer cell invasion into local fat may be a first step on cancer progression and systemic disease in TNBC.
Life-Long Coral Skeletal Acclimatization at CO2 Vents in Papua New Guinea Reveals Species- and Environment-Specific Effects
Authors: Prada Fiorella , Brizi Leonardo , Franzellitti Silvia , Mengoli Stefano , Fermani Simona , Polishchuk Iryna , Baraldi Nicola , Ricci Francesco , Palazzo Quinzia , Caroselli Erik , Pokroy Boaz , Giorgini Loris , Dubinsky Zvy , Fantazzini Paola , Falini Giuseppe , Goffredo Stefano , Fabricius Katharina 
Published: January 2021

Abstract
The responses of corals and other marine calcifying organisms to ocean acidification (OA) are variable and span from no effect to severe responses. Here we investigated the effect of long-term exposure to OA on skeletal parameters of four tropical zooxanthellate corals living at two CO2 vents in Papua New Guinea, namely in Dobu and Upa Upasina. The skeletal porosity of Galaxea fascicularis, Acropora millepora, and Pocillopora damicornis was higher (from 17% to 38%, depending on the species) at the seep site compared to the control only at Upa Upasina. Massive Porites showed no differences at any of the locations. Pocillopora damicornis also showed a ~ 7% decrease of micro-density and an increase of the volume fraction of the larger pores, a decrease of the intraskeletal organic matrix content with an increase of the intraskeletal water content, and no variation in the organic matrix related strain and crystallite size. The fact that the skeletal parameters varied only at one of the two seep sites suggests that other local environmental conditions interact with OA to modify the coral skeletal parameters. This might also contribute to explain the great deal of responses to OA reported for corals and other marine calcifying organisms.
Prevalence of Non-alcoholic Fatty Liver Disease and Its Associated Factors in Admixed Individuals With Type 1 Diabetes: a Cross-sectional Study in a Tertiary Care Center in Brazil
Authors: Barros Bianca Senger Vasconcelos , Monteiro Fernanda Cruz , Terra Carlos , Gomes Marilia Brito 
Published: January 2021

Abstract
Background: Data on non-alcoholic fatty liver disease (NAFLD) in admixed individuals with type 1 diabetes (T1D) is lacking. We investigated NAFLD in an admixed population with T1D from a tertiary care center in Brazil. Methods: Ninety-five participants with T1D, aged 39 ± 13 years, with disease duration of 21 ± 9 years, being 55 (57.9%) females, from a university hospital in Rio de Janeiro, were screened for NAFLD with hepatic ultrasound (US) and transient elastography (TE). Results: Prevalence of NAFLD was, respectively, 12.6% and 16.8% when US and TE were used. Fibrosis was present in 8% of participants. A total of 31.6% of participants had at least one of the hepatic exams altered, which was associated with higher anthropometric measurements, presence of metabolic syndrome and higher triglycerides levels, even within the normal range.Conclusion: In our study, prevalence of NAFLD in US approximates from the one found with TE. Screening should be reserved for participants with T1D and metabolic syndrome, as this was the main factor associated with NAFLD. Triglycerides levels were the only component of metabolic syndrome associated with NAFLD. Further studies are necessary to determine the best screening strategy for NAFLD in individuals with T1D from admixed populations.
Weight regain after Bariatric Surgery increases the request for Traditional Chinese Medicine Results from the cross-sectional BARBQTCM survey
Authors: Walker Benedict , Kessler Ulf , Kordasz Marcin , Rothe Steffi , Tsai Catherine , Zehetner Jörg , Panhofer Peter 
Published: January 2021

Abstract
Background: To investigate patient attitudes towards Traditional Chinese Medicine (TCM) after bariatric surgery with special emphasis on postoperative weight regain (WR).Methods: We retrospectively compared experiences with Complementary Additive Medicine (CAM) and the desire for additional TCM between patients with stable weight (SW) and WR by means of the disease-specific BARBQTCM survey during postoperative follow-up in a single bariatric center.Results: Out of 467 participating patients, 150 (32.1%) had WR after bariatric surgery. Median age was 49 years and 78.4% of the patients were female. 233 patients (49.9%) had already had experience with CAM and 125 (26.8%) with TCM. 381 patients (81.6%) were interested in using TCM at the time of the survey. Acceptance of TCM was lower in men (OR 0.35, P<0.01) and decreased with age (OR 0.95, P<0.001). Overall, the request for TCM was lower in the SW cohort compared to the WR group (77.3% versus 90.7%; OR 0.29, P<0.01). There was a positive correlation between preoperative experience with CAM and postoperative request for TCM (OR 5, P<0.001). The proportion of patients who were interested in outpatient TCM twice or more monthly was higher in patients with WR than in those with SW (94.0% versus 84.2%, P<0.01).Conclusions: The overall acceptance of CAM and TCM was high in our bariatric surgical patients. WR increased the rate of acceptance of TCM. We conclude that CAM and TCM should be offered during postoperative follow-up after bariatric surgery as part of an integrative medical approach.
Assessment and Documentation of Indigenous and Introduced Soil and Water Conservation Measures in Some Parts of Southern Ethiopia
Authors: Kassa Dawit Kanito , Bekele Tsegaye , Dawit Tesfaye , T/yohannes Seyfu 
Published: January 2021

Abstract
BackgroundIn Ethiopia, soil and water conservation activities were started after the famines of 1973 and 1985. Following this, different conservation measures were implemented by the government, non-government organizations, and the community to facilitate the optimum level of production from a given area of land while keeping soil loss below a critical value. This study was conducted at Wolaita, Hadiya, Kambata Tambaro, and Dawro Zones of Southern Ethiopia. It was designed to identify indigenous and introduced soil and water conservation practices, measure and describe identified practices, and document the identified practice for further reference. ResultsThe result revealed that three types of soil and water conservation practices namely biological, physical, and agronomical were implementing in the study areas. The result from the technical evaluation showed that soil bund and fanya juu has channel depth and embankment height lower than the recommended dimension. The result also indicated that less attention was given to indigenous soil and water conservation practices. Besides, the sustainability gap in constructed conservation practices was widely perceived. ConclusionsFarmers in the study area are well acquainted with soil erosion, its cause, and consequent reduction in land productivity. They confirm that implementing SWC measures are insurance to sustain and boost soil fertility and land productivity. In southern Ethiopia government and peoples were invested much more time, money, knowledge, and manpower to construct SWC measures. But, year by year structures get damage and yet seem not substantial to achieve the sector of sustainable agriculture. Besides, the study revealed that identified practices such as traditional cutoff drain, fanya juu, soil bund, stone-faced soil bund, fanya juu, brushwood, and gabion check-dams have technical, social, and institutional difficulties. The major gap observed with indigenous conservation practices is, it had been taken less attention by different stakeholders including GOs, NGOs, and research institutes. As a result, their respective dimension was not modified, effective measures were not up-scaled and circulated to other areas having similar agroecology and farming system. Thus, apparently, traditional conservation measures are aggravating soil erosion and their construction did not consider the downstream effect on dwelling community, water bodies, and aquatic lives.
Polyethylenimine-based Magnetic Nanocomplexes Enhanced Cisplatin Toxicity on Ovarian Cancer Cells in Presence of Static Magnetic Field
Authors: Ashoori Faranak , Hajipour-Verdom Behnam , Satari Mohammad , Abdolmaleki Parviz 
Published: January 2021

Abstract
BackgroundThe drug resistance of cancer cells is a major problem in the chemotherapy. Cisplatin (CIS) is one of the most effective chemotherapeutics used for ovarian cancer. Resistance to different chemo-drugs has developed over times. Here, we investigated the experimental approach to increase the CIS cytotoxicity and overcoming cell resistance through nanoparticle-based combination treatments. Polyethylenimine (PEI)-based magnetic iron oxide nanocomplexes were used to transfer the drugs in both genetically matched CIS-resistant (A2780CP) and -sensitive (A2780) ovarian cancer cells in presence of 20 mT static magnetic field. Magnetic nanoparticles (MNPs) were synthesized and bonded to PEI cationic polymer that leads to formation of binary complexes (PM). In the following, binding of CIS to PM binary complexes two including ternary complexes PM/C (PEI-MNP/CIS) and PMC (PEI-MNP-CIS) formed. ResultsOur results showed that the CIS cytotoxicity increased at different concentrations of CIS and PEI in all of binary and ternary treatments overtimes. Furthermore, CIS induced S and G2/M phase’s cell cycle arrest as well as reactive oxygen species (ROS) production in the both cell lines. Ternary complexes enhanced apoptosis more than binary complexes in the treated-cells. ConclusionsPEI-based magnetic noncomplex can be considered as a novel carrier for increasing the CIS cytotoxicity and probably overcoming drug resistance in ovarian cancer cells.
The Combined Anti-inflammatory Strategy of Beta-2 Adrenergic Agonist and Glucocorticoid on the Laying Hen Model of Ovarian Cancer: the Immune Traits and Ovarian Inflammatory Functions
Authors: Hatefi Ali , Shahneh Ahmad Zare , Pirsaraie Zarbakht Ansari , Alizadeh Ali Mohammad , Atashnak Mohammad Pouya , Masoudi Reza , Pio Frederic 
Published: January 2021

Abstract
Background: Ovarian cancer known as one of the most lethal gynecological malignancies mainly in older women, has been documented to link with chronic inﬂammation. Objective: This study was aimed to evaluate the combined strategy of glucocorticoid (GC) Fluticasone and beta-2 adrenergic agonist (BAA) Salmeterol on the ovarian inflammatory functions of the laying hen as a model of women ovarian cancer. Methods: White Leghorn hens aged 92 weeks were used for four weeks to be supplemented by individual of GC and BAA administration and their combination at three ratios GC:BAA 1:4 (GC+BAA1), 1:2 (GC+BAA2), and 1:1 (GC+BAA3), and beta blocker (BB) Propranolol. Ovulation rate and follicular growth were determined based on laying frequency and visual evaluation, respectively, the mRNA expressions of follicular beta-2 adrenergic receptor (β2ADR), cyclooxygenases (COX) 1 and 2, and cytokines were measured by real-time PCR. The plasma concentration of ovarian hormones cellular and humoral immune responses were measured via ELISA, neutrophil (heterophil) to lymphocyte ratio (NLR), and sheep red blood cell (SRBC) test, respectively. Results: As compared to control, combination groups of GC+BAA1 and 2 brought about a significant decrease in the mRNA expression of β2ADR, COX-2, and cytokines (P< 0.05). The ovulation rate was reduced in all combination ratios (P< 0.05). A signiﬁcant reduction was observed in the plasma estradiol content on GC+BAA groups (P< 0.05) and the content of progesterone and androgen were statistically similar in some of these groups. Although NLR was similar in GC+BAA2 and 3, these groups had more content in the whole immunoglobulin (Ig) and IgM (P< 0.05). The results indicated that body weight and food consummation were decreased in all of the combination groups (P< 0.05). Conclusion: The GC and BAA combination may result in a signiﬁcant reduction in some of the boosting factors on ovarian cancer, like ovulation intensity, pro-inflammatory mediators, and some of the ovarian steroid hormones that could define as the therapeutic approaches for the chronic inflammation-based carcinomas like ovarian cancer.
Minimally Invasive Pedicle Subtraction Osteotomy via Tubular Retractor for Adult Rigid Spinal Kyphoscoliosis: a Case Report and Review of the Literature
Authors: Xing ZeJun , Ma Zhuo , Ma Xun 
Published: January 2021

Abstract
Objective To describe a new technique, minimally invasive pedicle subtraction osteotomy (MIS PSO) via tubular retractor, for severe rigid adult spinal deformity.Methods We present a case with severe rigid thoracolumbar kyphoscoliosis and describe the surgical procedure , to which this patient responded favorably.Results No surgical complications were seen and estimated blood loss was 600ml. The operation process time was about 5 hours. She received 2 unit of packed red blood cells intraoperatively. VAS was 8.5 points at pre-operation and 2 .0 points at 2 years follow-up. The ODI score improved from 33 to 9. Fusion was confirmed by wedging of L2 vertebral body on CT reconstructive films. Routine x-rays showed that spinal imbalance was corrected and maintained at the final follow up. Maximal ambulation distance increased to 5000 feet at 2 years follow-up.Conclusion A MIS PSO is a feasible option for the treatment of certain subtypes of adult spinal deformity. It is technically feasible that although limited by learning curve of minimally invasive tubular technique and open PSO technique. Both operation skill of the surgeons and proper patient selection is the key to successfully treating adult patients with deformity using MIS PSO.
Gene expression-based immune infiltration analyses of renal cancer and their associations with survival outcome
Authors: Chen Lei , Yin Liang , Qi Zilong , Li Jinmin , Wang Xinning , Ma Kun , Liu Xiangyang 
Published: January 2021

Abstract
Background: Renal cancer is a common malignant tumor with an increasing incidence rate. Methods: In this study, based on the gene expression profiles, we analyzed the compositions of tumor-infiltrating immune cells (TIICs) in renal cancer and paracancerous samples using CIBERSORT. The proportions of 22 TIICs subsets in 122 paired renal carcinoma and paracancerous samples, and 224 Wilms tumor (WT) samples varied between intragroup and intergroup. Results: After analyzed the difference of TIICs composition between renal cancer and paired paracancerous samples, we found that M0 macrophages and CD8 T cells were significantly elevated, while naive B cells were significantly decreased in renal cancer samples compared with paracancerous samples. Survival analysis showed that high overall TIICs proportion , the low proportion of resting mast cells and the high proportion of activated memory CD4 T cells were associated with poor prognosis of renal cancer patients. In addition, 3 clusters were identified by hierarchical clustering analysis, and they presented a distinct prognosis. Cluster 1 had superior survival outcomes, while cluster 2 had an inferior survival outcome. Conclusions: Our study indicated that overall TIICs proportion, certain TIICs subset proportion, including resting mast cells and activated memory CD4 T cells, and distinct cluster patterns were associated with the prognosis of renal cancer, which was significant for the clinical surveillance and treatment of renal cancer.
KLF5-mediated Eppk1 expression promotes cell proliferation in cervical cancer via the p38 signaling pathway
Authors: Ma Dong , Pan Zhe , Chang Quan , Zhang Jin-jin , Liu Xiao , Hua Na , Li Guo-Hua 
Published: January 2021

Abstract
Background: Epiplakin1 (Eppk1) is part of epidermal growth factor (EGF) signal and takes part in reorganization of cytoskeleton and cell proliferation. However, the role of Eppk1 in cervical cancer (CC) remains unknown.Methods: To express Eppk1 and KLF5 and their correlation, we used RNA-sequence, RT-qPCR, TCGA database and immunofluorescence staining in vitro and in different pathological cervical tissues. In CC cell lines, we tested adenovirus-mediated over expression or knockdown of KLF5 and siRNA-mediated knockdown of Eppk1 and a suiting assessment of cell proliferation and cell signaling by western blot and CCK8 tests. We studied the mechanism by which KLF5 regulates Eppk1 expression by reporter gene test and chromatin immunoprecipitation test.Results: Eppk1 expression promoted in CC tissues and cell lines compared with increased KLF5 expression. The results of immunofluorescence staining further showed the increased co-expression of Eppk1 and KLF5 correlated substantially with tumorigenesis in cervical tissues. Overexpression of KLF5 significantly increased Eppk1 expression at transcription and translation levels. Conversely, the knockdown of KLF5 by siRNA against KLF5 decreased Eppk1 expression. Mechanically, KLF5 activated Eppk1 transcription by direct binding to the Eppk1 promoter. Gain- and loss-of-function experiments reported that KLF5 promoted cell proliferation in Hela partly dependent on Eppk1 upregulation. Besides, KLF5-mediated activation of p38 signaling significantly decreased after Eppk1 knockdown compared with decline of proliferation, suggesting that Eppk1 lies upstream of p38 signaling affecting cell proliferation. Finally, Eppk1 expression is positively correlated with tumor size in clinicopathological features of CC. Conclusions: Eppk1 may be an effective therapeutic target for affecting p38 signaling pathway and cell proliferation in cervical cancer.
Men perspectives on attending antenatal care visits together with their pregnant partners in Misungwi district, rural Tanzania: a qualitative study
Authors: Boniphace Maendeleo , Matovelo Dismas , Laisser Rose , Swai Hadija , Yohana Victoria , Tinka Sylvia , Mwaikasu Lusako , Mercader Hannah , Brenner Jennifer L , Mitchell Jennifer 
Published: January 2021

Abstract
Background: Mens’attendance with their pregnant partners at facility-based antenatal care (ANC) visits is important for maternal and child health and gender equality yet remains uncommon in parts of rural Tanzania. This study examined men’s perspectives on attending ANC with their pregnant partners in Misungwi District, Tanzania.Methods: Twelve individual interviews and five focus group discussions were conducted using semi-structured questionnaires with fathers, expectant fathers, and in-depth interviews were done to health providers, volunteer community health workers, and village leaders. Interviews were recorded and transcribed in Swahili and later translated to English. The research team conducted thematic analysis to identify common themes among interviews. Results: We identified two broad themes on the barriers to male attendance at facility-based ANC visits: (1) Perceived exclusion during ANC visits among men (2) Traditional gender norms resulting to low attendance among men.Conclusion: Attendance at health facility for ANC visits by men with their pregnant partners in the study areas were challenged by structural and local cultural norms. At the facility men were uncomfortable to sit with women due to lack of specific waiting area for men and that they perceived to be neglected. Local cultural norms demanded women to have secrecy in pregnancy while men perceived not to have a role of being with their partners during ANC visits.
Combined Macromolecule biomaterials together with fluid shear stress promote the osteogenic differentiation capacity of equine adipose -derived mesenchymal stem cells
Authors: Elashry Mohamed I. , Baulig Nadine , Wagner Alena-Svenja , Klymiuk Michele C. , Kruppke Benjamin , Hanke Thomas , Wenisch Sabine , Arnhold Stefan 
Published: January 2021

Abstract
Background: Combination of mesenchymal stem cells (MSCs) and biomaterials is a rapidly growing approach in regenerative medicine particularly for chronic degenerative disorders including osteoarthritis and osteoporosis. The present study examined the effect of biomaterial scaffolds on equine adipose derived MSCs morphology, viability, adherence, migration and osteogenic differentiation.Methods: MSCs were cultivated in conjunction with collagen CultiSpher-S Microcarrier (MC), nanocomposite xerogels B30 and combined B30 with strontium (B30Str) biomaterials in osteogenic differentiation medium either under static or mechanical fluid shear stress (FSS) culture conditions. The data were generated by histological means, live cell imaging, cell viability, adherence and migration assays, semi-quantification of alkaline phosphatase (ALP) activity and quantification of the osteogenic markers runt related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) expression.Results: The data revealed that combined mechanical FSS with MC but not B30 enhanced MSCs viability and promoted their migration. Combined osteogenic medium with MC, B30 and B30Str increased ALP activity compared to cultivation in basal medium. Osteogenic induction with MC, B30 and B30Str resulted in diffused matrix mineralization. The combined osteogenic induction with biomaterials under mechanical FSS increased Runx2 protein expression either in comparison to those cells cultivated in BM or those cells induced under static culture. Runx2 and ALP expression was upregulated following combined osteogenic differentiation together with B30 and B30Str regardless of static or FSS culture.Conclusions: Taken together, the data revealed that FSS in conjunction with biomaterials promoted osteogenic differentiation of MSCs. This combination may be considered as a marked improvement for clinical applications to cure bone defects.