bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cerambycid Communities and Their Associated Hymenopteran Parasitoids From Major Hardwood Trees in Delaware: Implications for Biocontrol of Invasive Longhorned Beetles
Authors: Golec Julian R , Aparicio Ellen , Wang Xingeng , Duan Jian J , Fuester Roger W , Tatman Daria , Kula Robert R 
Published: December 2019

AbstractCerambycidae provide important ecological services in forests yet cause economic damage when they infest living trees. Parasitoids can regulate woodborer populations, providing considerable control of pest cerambycids. Identifying parasitoids of native cerambycids may be useful in managing cerambycid outbreaks and aid in new-association biocontrol of exotic invasive cerambycids. We investigated Cerambycidae and associated hymenopteran parasitoid communities infesting Acer rubrum, Pinus virginiana, and Carya tomentosa from a forest in Delaware from 2005 to 2012. Cerambycid abundance, diversity, and richness, as well as parasitoid abundance, were measured by collecting trees in different conditions: felled, girdled, and naturally infested. Effect of edge or interior red maple on cerambycid abundance, diversity, and richness was examined. Over 14,500 cerambycids of 56 species and 38 genera were collected during the 7-yr period. Eleven species represented 95% of all cerambycids collected. Treatment only affected red maple, showing increased cerambycid richness and diversity from naturally infested trees. Cerambycid richness and diversity were two times greater on hickory than other species when combining girdled and felled treatments. Over 19,000 parasitic Hymenoptera of 12 families emerged from woodborer-infested wood with &gt;70% of individuals belonging to Braconidae. Thirteen known species, and two unknown species, of Braconidae were identified from a subsample of 495 specimens; Ontsira mellipes (Ashmead) (Hymenoptera: Braconidae) and Rhoptrocentrus piceus Marshall (Hymenoptera: Braconidae) were the most abundant. This study provides fundamental information on native parasitoids associated with Cerambycidae, including cerambycid larval host associations. Parasitoids identified herein should be investigated for potential adaptation to invasive Cerambycidae to benefit invasive woodborer management.
Successful treatment with rivaroxaban of an extended deep vein thrombosis complicated by pulmonary embolism in a patient with familial antithrombin III deficiency: a case report
Authors: Appignani Marianna , Sciartilli Adolfo , Caputo Marcello , Di Girolamo Enrico 
Published: December 2019

AbstractBackground Patients with low levels of antithrombin III (AT III) are at an increased risk of developing arteriovenous thromboembolic disease.Case summary We report a case of a 28-year-old woman who presented with a 1-week history of spontaneous right calf pain and swelling. A heterozygous AT III deficiency, phenotypically expressed as deep vein thrombosis, was reported in the patient’s mother and sister. Blood workup revealed residual AT III activity at 58% with normal protein C and protein S levels. Computed tomographic angiography (CTA) revealed subsegmental bilateral pulmonary embolism (PE) and deep vein thrombosis in the right leg extending into the inferior vena cava up to the confluence of the left renal vein. Placement of an inferior vena cava filter was not considered. Given the patient’s haemodynamic stability, anticoagulant therapy with 15 mg of rivaroxaban twice a day was initiated instead. Echocardiography after 10 days of treatment revealed complete resolution of the thrombus located in the inferior vena cava, while CTA revealed complete resolution of the PE.Discussion Patients with AT III deficiency are likely to be heparin-resistant and will require higher heparin doses or the administration of AT III replacement therapy for the treatment of thrombosis, both of which are associated with an increased risk for haemorrhagic complications. Direct factor Xa inhibition by rivaroxaban provided an alternative mechanism for anticoagulation, which was found to be particularly useful in this patient with familial AT III deficiency, deep vein thrombosis, and PE.
Reduced form evidence on belief updating under asymmetric information—consumers’ response to wine expert opinions
Authors: Bonnet Céline , Hilger James , Villas-Boas Sofia B 
Published: December 2019

AbstractWe estimate the effect of quality labels on purchases through a retail field experiment. Utilising product-level panel scanner and product characteristic data for both labelled and unlabelled wines we estimate the average and heterogeneous effect on purchases. Consistent with earlier work, we find an average effect that is positively correlated with scores. We advance the consumer belief and product information literature on two fronts. First, higher scores matter more for lower priced products. Second, spillover effects impact sales of untreated wines; these effects can be positive or negative and are impacted by the average score and label converge within brand.
Assessing volume and variation of low-value care practices in the Netherlands
Authors: Kool Rudolf Bertijn , Verkerk Eva Wilhelmina , Meijs Jill , van Gorp Niels , Maessen Martijn , Westert Gert , Peul Wilco , van Dulmen Simone 
Published: December 2019

Abstract

Background
There have been contributions to quantify the volume of low-value care practices in the USA, Canada and Australia but we have no knowledge about the volume in Europe. The purpose of this study was to assess the volume and variation of Dutch low-value care practices.

Methods
We conducted a cross-sectional study with data of a Dutch healthcare insurance company from general practioners (GP’s) and hospitals in the Netherlands from 2016. We used all billing claims made by healthcare providers of 3.5 million Dutch inhabitants. We studied Choosing Wisely recommendations in order to select low-value care practices. We used the percentage low-value care practices per hospital and number of low-value care practices per GP as outcomes.

Results
We assessed the volume of low-back imaging by GPs, screening of patients over 75 years for colorectal cancer and diagnosing varices with Doppler or Plethysmography. We found that 0.4% (range 0–7%) of the eligible patients received low-value screening for colorectal cancer and 8.0% (range 0–88%) of eligible patients received low-value diagnosing of varices. About 52.4% of the GPs ordered X-rays and 11.2% ordered magnetic resonance imagings of the lumbosacral spine. Most healthcare providers did not provide the measured low-value care practices. However, 1 in 12 GPs ordered at least one low-back X-ray a week.

Conclusions
The three Choosing Wisely recommendations showed a lot of practice variation; many healthcare providers did not order these low-value diagnostic tests; a minor part did order a substantial amount, low-back spine radiology in particular. These healthcare providers should start reducing these activities.
Dietary Partitioning in Two Co-occurring Caecilian Species (Geotrypetes seraphini and Herpele squalostoma) in Central Africa
Authors: Kouete M T , Blackburn D C 
Published: December 2019

SynopsisTrophic interactions among fossorial vertebrates remain poorly explored in tropical ecosystems. While caecilian species can co-occur, whether and how sympatric species partition dietary or other resources are largely unknown. Based on specimens collected during field surveys in southern Cameroon, we conducted a dietary analysis of two co-occurring caecilian species, Geotrypetes seraphini and Herpele squalostoma. We find a negligible overlap in the adult diets of these two species. Earthworms dominated the diet of adult G. seraphini, whereas we found that mole crickets were the most frequent prey items in adult H. squalostoma. The dietary breadth of adult G. seraphini is smaller than that of H. squalostoma, which consumes a variety of hard-bodied prey including mole crickets, cockroaches, beetles, and crabs. Juvenile diets were similar between these species and mostly contained earthworms and ants. We did not detect significant ontogenetic dietary shifts in either species, though adults generally consumed a broader diversity of prey. As adults, G. seraphini and H. squalostoma may partition prey categories by consuming soft-bodied and hard-bodied prey, respectively. Because most caecilians are likely opportunistic predators, we expect that sympatric species partition dietary resources either by preference for different soil layers or ability to consume different prey categories.
Identification and Evaluation of Reference Genes for Normalization of Gene Expression in Developmental Stages, Sexes, and Tissues of Diaphania caesalis (Lepidoptera, Pyralidae)
Authors: Wang Zheng , Meng Qianqian , Zhu Xi , Sun Shiwei , Liu Aiqin , Gao Shengfeng , Gou Yafeng 
Published: December 2019

Abstract
Diaphania caesalis (Walker) is an important boring insect mainly distributed in subtropical and tropical areas and attacked tropical woody grain crops, such as starchy plants of Artocarpus. Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful approach for investigating target genes expression profiles at the transcriptional level. However, the identification and selection of internal reference genes, which is often overlooked, is the most vital step before the analysis of target gene expression by qRT-PCR. So far, the reliable internal reference genes under a certain condition of D. caesalis have not been investigated. Therefore, this study evaluated the expression stability of eight candidate reference genes including ACT, β-TUB, GAPDH, G6PDH, RPS3a, RPL13a, EF1α, and EIF4A in different developmental stages, tissues and sexes using geNorm, NormFinder and BestKeeper algorithms. To verify the stability of the recommended internal reference genes, the expression levels of DcaeOBP5 were analyzed under different treatment conditions. The results indicated that ACT, RPL13a, β-TUB, RPS3a, and EF1α were identified as the most stable reference genes for further studies on target gene expression involving different developmental stages of D. caesalis. And ACT and EIF4A were recommended as stable reference genes for different tissues. Furthermore, ACT, EF1α, and RPS3a were ranked as the best reference genes in different sexes based on three algorithms. Our research represents the critical first step to normalize qRT-PCR data and ensure the accuracy of expression of target genes involved in phylogenetic and physiological mechanism at the transcriptional level in D. caesalia.
Impact of forest fire on soil seed bank composition in Himalayan Chir pine forest
Authors: Konsam Bobbymoore , Phartyal Shyam S , Todaria Nagendra P 
Published: December 2019

AbstractAimsTo explain how plant community copes with a recurring anthropogenic forest fire in Himalayan Chir pine forest, it is important to understand their postfire regeneration strategies. The primary aim of the study was to know: (i) how fire impact soil seed bank composition and (ii) how much soil seed bank composition differs with standing vegetation after the forest fire.MethodsSoil samples were collected from burned and adjoining unburned sites in blocks using three layers down to 9 cm depth immediately after a forest fire and incubated in the net-house for seedling emergence. Same sites were revisited during late monsoon/early autumn season to know the species composition of standing vegetation recovered after a forest fire.Important FindingsSoil contained viable seeds of &gt;70 species. The average seed bank density was 8417 and 14 217 seeds/m2 in the burned and unburned site, respectively. In both sites, it decreased with increasing soil depth. Overall fire had no significant impact on seed density; however, taking individual layers into consideration, fire had a significant impact on seed density only in the uppermost soil layer. The species richness of soil seed bank and standing vegetation was 73 and 100, respectively (with 35 shared species), resulting in a similarity of about 40%. In contrast, &gt;80% species in soil seed bank was found similar between burned and unburned sites. Further, there were no significant differences in species richness of standing vegetation in burned (87 spp.) and unburned (78 spp.) sites. Our results showed that fire had an insignificant impact on soil seed bank composition and restoration potential of a plant species from seeds. The understory herb and shrub plant community’s ability to form a fire-resistant viable soil seed bank and capable to recover in the postfire rainy season, explains how they reduce the risk of recurring fire damage in maintaining their population.
Acid and heat fastness in microsporidia: How acid fast are acid fast microsporidium?
Authors: Mittal Ruchi , Agarwal Sunil , Muduli Kalandi Ch , Sahu Srikant , Das Sujata 
Published: December 2019

Abstract
Microsporidia are obligate spore-forming microorganisms with strong resemblance to fungi and can affect almost every organ system in immunocompetent or immunocompromised individuals. Mixed infections are also reported in immunocompromised hosts. Microsporidial spores show marked morphological variations and the small and slender forms can resemble bacilli. Modified Zeihl Neelsen (ZN) stain, cold method demonstrates them as bright red in color, leaving several spores blue or incompletely stained; thus, they are reported as weakly or variably acid fast. Variability in staining results with ZN stain and considering the fact that Mycobacterium tuberculosis, the commoner bug in developing countries is identified by its resistance to stronger acids on ZN staining, authors wished to demonstrate acid and heat fastness in microsporidium using corneal tissue specimens. Microsporidial spores stained bright red in color with conventional ZN stain, demonstrated strong acid fastness, and interestingly the staining results improved on heating. Thus, the authors conclude that they are strongly acid and heat fast and care must be warranted so that they are not misdiagnosed as Mycobacterium or other acid-fast organisms. Careful observation of morphology, battery of special stains, and molecular diagnostics should be advocated for diagnostic confirmation. To the best of the authors’ knowledge, this is the first explicit report on acid and heat fastness on microsporidial spores.
Large-scale star formation in Auriga region
Authors: Pandey A K , Sharma Saurabh , Kobayashi N , Sarugaku Y , Ogura K 
Published: December 2019

ABSTRACT
New observations in the VI bands along with archival data from the 2MASS and WISE surveys have been used to generate a catalogue of young stellar objects (YSOs) covering an area of about 6° × 6° in the Auriga region centred at l ∼ 173° and b ∼ 1.5°. The nature of the identified YSOs and their spatial distribution are used to study the star formation in the region. The distribution of YSOs along with that of the ionized and molecular gas reveals two ring-like structures stretching over an area of a few degrees each in extent. We name these structures as Auriga Bubbles 1 and 2. The centre of the Bubbles appears to be above the Galactic mid-plane. The majority of Class I YSOs are associated with the Bubbles, whereas the relatively older population, i.e. Class ii objects are rather randomly distributed. Using the minimum spanning tree analysis, we found 26 probable subclusters having five or more members. The subclusters are between ∼0.5 and ∼3 pc in size and are somewhat elongated. The star formation efficiency in most of the subcluster region varies between 5 ${{\ \rm per\ cent}}$ and 20 ${{\ \rm per\ cent}}$ indicating that the subclusters could be bound regions. The radii of these subclusters also support it.
Persistence of Immune Responses Through 36 Months in Healthy Adults After Vaccination With a Novel Staphylococcus aureus 4-Antigen Vaccine (SA4Ag)
Authors: Creech C Buddy , Frenck Robert W , Fiquet Anne , Feldman Robert , Kankam Martin K , Pathirana Sudam , Baber James , Radley David , Cooper David , Eiden Joseph , Gruber William C , Jansen Kathrin U , Anderson Annaliesa S , Gurtman Alejandra 
Published: December 2019

Abstract

Background
Staphylococcus aureus causes serious health care– and community-associated disease, requiring improved preventive measures such as vaccines. The investigational S. aureus 4-antigen vaccine (SA4Ag), comprising capsular polysaccharide serotypes 5 and 8 (CP5 and CP8) conjugated to CRM197, recombinant mutant clumping factor A (rmClfA), and recombinant manganese transporter protein C (rP305A or rMntC), was well tolerated, inducing robust functional immune responses to all 4 antigens through 12 months postvaccination. This is a serological extension study through 36 months postvaccination.

Methods
In 2 previous studies, healthy adults received SA4Ag, SA3Ag (without rMntC), or placebo; serology was also assessed at ~24 and ~36 months postvaccination. Functional immune responses (antibody responses that facilitate killing of S. aureus or neutralize S. aureus virulence mechanisms) were assessed with opsonophagocytic activity killing assays (CP5 or CP8) and a fibrinogen-binding inhibition assay (ClfA). A competitive Luminex immunoassay assessed ClfA and rMntC responses. Adverse events within 48 hours of blood draw were recorded.

Results
Four hundred forty subjects (18–64 years old, 255; 65–85 years old, 185) were enrolled. At 24 and 36 months postvaccination, subjects receiving SA4Ag had substantially higher geometric mean titers (GMTs) for CP5, CP8, and ClfA vs baseline; geometric mean fold rises (GMFRs) from baseline to month 36 were 2.7–8.1. For rMntC, 36-month GMTs declined from peak levels but remained above baseline for all SA4Ag groups; GMFRs from baseline to month 36 were 1.8 and 1.5 in the younger and older cohorts, respectively.

Conclusions
Persistent functional immune responses to S. aureus antigens were observed through 36 months in healthy adults.

ClinicalTrials.gov
NCT01643941 and NCT01364571.
Robotic Surgical Assistant Rehearsal: Combining 3-Dimensional-Printing Technology With Preoperative Stereotactic Planning for Placement of Stereoencephalography Electrodes
Authors: Bonda David J , Pruitt Rachel , Goldstein Todd , Varghese Anish , Shah Amar , Rodgers Shaun 
Published: December 2019

Abstract

BACKGROUND
The use of frameless stereotactic robotic technology has rapidly expanded since the Food and Drug Administration's approval of the Robotic Surgical Assistant (ROSA) in 2012. Although the use of the ROSA robot has greatly augmented stereotactic placement of intracerebral stereoelectroencephalography (sEEG) for the purposes of epileptogenic focus identification, the preoperative planning stages remain limited to computer software.

OBJECTIVE
To describe the use of a 3-dimensionally (3D)-printed patient model in the preoperative planning of ROSA-assisted depth electrode placement for epilepsy monitoring in a pediatric patient.

METHODS
An anatomically accurate 3D model was created and registered in a preoperative rehearsal session using the ROSA platform. After standard software-based electrode trajectory planning, sEEG electrodes were sequentially placed in the 3D model.

RESULTS
Utilization of the 3D-printed model enabled workflow optimization and increased staff familiarity with the logistics of the robotic technology as it relates to depth electrode placement. The rehearsal maneuvers enabled optimization of patient head positioning as well as identification of physical conflicts between 2 electrodes. This permitted revision of trajectory planning in anticipation of the actual case, thereby improving patient safety and decreasing operative time.

CONCLUSION
Use of a 3D-printed patient model enhanced presurgical positioning and trajectory planning in the placement of stereotactic sEEG electrodes for epilepsy monitoring in a pediatric patient. The ROSA rehearsal decreased operative time and increased efficiency of electrode placement.
Pamidronate as first-line treatment of hypercalcemia in neonatal subcutaneous fat necrosis: A case series
Authors: Chesover Alexander D , Harrington Jennifer , Mahmud Farid H 
Published: December 2019

Abstract

Background
Subcutaneous fat necrosis (SCFN) can be complicated by severe hypercalcemia, which is frequently asymptomatic. Nephrocalcinosis is associated with hypercalcemia and, in other clinical settings, has been linked to furosemide and glucocorticoid use. First-line bisphosphonate therapy treating hypercalcemia in neonatal SCFN is not well described.

Objectives
To describe the biochemical changes and risk of nephrocalcinosis in infants with hypercalcemia, secondary to neonatal SCFN, treated with initial pamidronate.

Methods
A retrospective chart review of five infants treated with initial pamidronate and without furosemide or glucocorticoids. Data were collected on the following: timing of presentation, therapeutic response, and presence of nephrocalcinosis.

Results
Hypercalcemia resolved after 2.8±1.7 days; this is compared to 7.6±2.8 days from previously reported cases utilising alternative therapies (P=0.012). There were no episodes of rebound hypercalcemia or hypocalcemia. Nephrocalcinosis was present in four of five cases. When including published cases, age at diagnosis was associated with presenting serum calcium (P=0.003) and nephrocalcinosis was associated with higher serum calcium (P=0.014) and time from SCFN to hypercalcemia diagnosis (P=0.002).

Conclusions
This retrospective case series demonstrates that first-line pamidronate treatment was effective and safe in the resolution of hypercalcemia. Nephrocalcinosis was observed, despite the avoidance of furosemide and glucocorticoid therapy, and associated with greater disease severity and duration of hypercalcemia.
The falling asleep process in adolescents
Authors: de Zambotti Massimiliano , Goldstone Aimee , Forouzanfar Mohamad , Javitz Harold , Claudatos Stephanie , Colrain Ian M , Baker Fiona C 
Published: December 2019

Abstract

Study Objectives
To investigate the pre-sleep psychophysiological state and the arousal deactivation process across the sleep onset (SO) transition in adolescents.

Methods
Data were collected from a laboratory overnight recording in 102 healthy adolescents (48 girls, 12–20 years old). Measures included pre-sleep self-reported cognitive/somatic arousal, and cortical electroencephalographic (EEG) and electrocardiographic activity across the SO transition.

Results
Adolescent girls, compared with boys, reported higher pre-sleep cognitive activation (p = 0.025) and took longer to fall asleep (p &lt; 0.05), as defined with polysomnography. Girls also showed a less smooth progression from wake-to-sleep compared with boys (p = 0.022). In both sexes, heart rate (HR) dropped at a rate of ~0.52 beats per minute in the 5 minutes preceding SO, and continued to drop, at a slower rate, during the 5 minutes following SO (p &lt; 0.05). Older girls had a higher HR overall in the pre-sleep period and across SO, compared to younger girls and boys (p &lt; 0.05). The EEG showed a progressive cortical synchronization, with increases in Delta relative power and reductions in Alpha, Sigma, Beta1, and Beta2 relative powers (p &lt; 0.05) in the approach to sleep, in both sexes. Delta relative power was lower and Theta, Alpha, and Sigma relative powers were higher in older compared to younger adolescents at bedtime and across SO (p &lt; 0.05).

Conclusions
Our findings show the dynamics of the cortical-cardiac de-arousing process across the SO transition in a non-clinical sample of healthy adolescents. Findings suggest a female-specific vulnerability to inefficient sleep initiation, which may contribute to their greater risk for developing insomnia.