bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of the central human and mouse platelet signaling cascade by systems biological analysis
Authors: Balkenhol Johannes , Kaltdorf Kristin V. , Mammadova-Bach Elmina , Braun Attila , Nieswandt Bernhard , Dittrich Marcus , Dandekar Thomas 
Published: December 2020

Abstract
Background
Understanding the molecular mechanisms of platelet activation and aggregation is of high interest for basic and clinical hemostasis and thrombosis research. The central platelet protein interaction network is involved in major responses to exogenous factors. This is defined by systemsbiological pathway analysis as the central regulating signaling cascade of platelets (CC).

Results
The CC is systematically compared here between mouse and human and major differences were found. Genetic differences were analysed comparing orthologous human and mouse genes. We next analyzed different expression levels of mRNAs. Considering 4 mouse and 7 human high-quality proteome data sets, we identified then those major mRNA expression differences (81%) which were supported by proteome data. CC is conserved regarding genetic completeness, but we observed major differences in mRNA and protein levels between both species. Looking at central interactors, human PLCB2, MMP9, BDNF, ITPR3 and SLC25A6 (always Entrez notation) show absence in all murine datasets. CC interactors GNG12, PRKCE and ADCY9 occur only in mice. Looking at the common proteins, TLN1, CALM3, PRKCB, APP, SOD2 and TIMP1 are higher abundant in human, whereas RASGRP2, ITGB2, MYL9, EIF4EBP1, ADAM17, ARRB2, CD9 and ZYX are higher abundant in mouse. Pivotal kinase SRC shows different regulation on mRNA and protein level as well as ADP receptor P2RY12.

Conclusions
Our results highlight species-specific differences in platelet signaling and points of specific fine-tuning in human platelets as well as murine-specific signaling differences.
Perceptions of care after end-of-treatment among younger women with different gynecologic cancer diagnoses – a qualitative analysis of written responses submitted via a survey
Authors: Mattsson Elisabet , Ljungman Lisa , Einhorn Kim , Sundström Poromaa Inger , Stålberg Karin , Wikman Anna 
Published: December 2020

Abstract
Background
Less attention has been given to younger adults’ psycho-oncology care needs than to children and older adults with cancer. The aim was to explore how care following end-of-treatment was perceived by women treated for different gynecologic cancer diagnoses during younger adulthood.

Methods
A sample of 207 women diagnosed with gynecologic cancer 2008 to 2016, aged 19–39 at time of diagnosis answered one open-ended question regarding important aspects of care after end-of-treatment. The written responses were analyzed with manifest content analysis and presented in relation to the women’s diagnoses, i.e., cervical (n = 130), ovarian (n = 57), and other gynecologic cancer diagnoses (n = 20).

Results
The analysis resulted in three categories: Unmet long-term supportive care needs, Satisfying long-term supportive care, and Health care organizational difficulties. Over half of the women (66.7%) described unmet care needs. The corresponding figures were 80.7, 63.1 and 50% for women diagnosed with ovarian, cervical and other gynecologic cancer diagnoses, respectively. Satisfying supportive care were described by approximately one quarter of the women (26.1%). Among women diagnosed with ovarian cancer 14% described satisfying supportive care. The corresponding figures were 26.9 and 30% for women diagnosed with cervical cancer and other gynecological diagnoses, respectively. Approximately one quarter of the women, irrespectively of diagnosis, described aspects related to health care organizational difficulties (28%).

Conclusions
The results highlight the importance of good quality care linked to the diagnosis and based on an understanding of the woman’s need, desire and expectation of support after end-of-treatment.
Arsenic nano complex induced degradation of YAP sensitized ESCC cancer cells to radiation and chemotherapy
Authors: Zhou Wei , Liu Meiyue , Li Xia , Zhang Peng , Li Jiong , Zhao Yue , Sun Guogui , Mao Weimin 
Published: December 2020

Abstract
Background
Increased reactive oxygen species (ROS) production by arsenic treatment in solid tumors showed to be effective to sensitize cancer cells to chemotherapies. Arsenic nano compounds are known to increase the ROS production in solid tumors.

Methods
In this study we developed arsenic–ferrosoferric oxide conjugated Nano Complex (As2S2–Fe3O4, AFCNC) to further promote the ROS induction ability of arsenic reagent in solid tumors. We screen for the molecular pathways that are affect by arsenic treatment in ESCC cancer cells. And explored the underlying molecular mechanism for the arsenic mediated degradations of the key transcription factor we identified in the gene microarray screen. Mouse xenograft model were used to further verify the synthetic effects of AFCNC with chemo and radiation therapies, and the molecular target of arsenic treatment is verified with IHC analysis.

Results
With gene expression microarray analysis we found Hippo signaling pathway is specifically affected by arsenic treatment, and induced ubiquitination mediated degradation of YAP in KYSE-450 esophageal squamous cell carcinoma (ESCC) cells. Mechanistically we proved PML physically interacted with YAP, and arsenic induced degradation PML mediated the degradation of YAP in ESCC cells. As a cancer stem cell related transcription factor, YAP 5SA over expressions in cancer cells are correlated with resistance to chemo and radiation therapies. We found AFCNC treatment inhibited the increased invasion and migration ability of YAP 5SA overexpressing KYSE-450 cells. AFCNC treatment also effectively reversed protective effects of YAP 5SA overexpression against cisplatin induced apoptosis in KYSE-450 cells. Lastly, with ESCC mouse xenograft model we found AFCNC combined with cisplatin treatment or radiation therapy significantly reduced the tumor volumes in vivo in the xenograft ESCC tumors.

Conclusions
Together, these findings suggested besides ROS, YAP is a potential target for arsenic based therapy in ESCC, which should play an important role in the synthetic effects of arsenic nano complex with chemo and radiation therapy.
Mitochondrial defects in the respiratory complex I contribute to impaired translational initiation via ROS and energy homeostasis in SMA motor neurons
Authors: Thelen Maximilian Paul , Wirth Brunhilde , Kye Min Jeong 
Published: December 2020

AbstractSpinal muscular atrophy (SMA) is a neuromuscular disease characterized by loss of lower motor neurons, which leads to proximal muscle weakness and atrophy. SMA is caused by reduced survival motor neuron (SMN) protein levels due to biallelic deletions or mutations in the SMN1 gene. When SMN levels fall under a certain threshold, a plethora of cellular pathways are disturbed, including RNA processing, protein synthesis, metabolic defects, and mitochondrial function. Dysfunctional mitochondria can harm cells by decreased ATP production and increased oxidative stress due to elevated cellular levels of reactive oxygen species (ROS). Since neurons mainly produce energy via mitochondrial oxidative phosphorylation, restoring metabolic/oxidative homeostasis might rescue SMA pathology. Here, we report, based on proteome analysis, that SMA motor neurons show disturbed energy homeostasis due to dysfunction of mitochondrial complex I. This results in a lower basal ATP concentration and higher ROS production that causes an increase of protein carbonylation and impaired protein synthesis in SMA motor neurons. Counteracting these cellular impairments with pyruvate reduces elevated ROS levels, increases ATP and SMN protein levels in SMA motor neurons. Furthermore, we found that pyruvate-mediated SMN protein synthesis is mTOR-dependent. Most importantly, we showed that ROS regulates protein synthesis at the translational initiation step, which is impaired in SMA. As many neuropathies share pathological phenotypes such as dysfunctional mitochondria, excessive ROS, and impaired protein synthesis, our findings suggest new molecular interactions among these pathways. Additionally, counteracting these impairments by reducing ROS and increasing ATP might be beneficial for motor neuron survival in SMA patients.
Recent advances in nanomaterial-modified electrical platforms for the detection of dopamine in living cells
Authors: Cho Yeon-Woo , Park Joon-Ha , Lee Kwang-Ho , Lee Taek , Luo Zhengtang , Kim Tae-Hyung 
Published: December 2020

AbstractDopamine is a key neurotransmitter that plays essential roles in the central nervous system, including motor control, motivation, arousal, and reward. Thus, abnormal levels of dopamine directly cause several neurological diseases, including depressive disorders, addiction, and Parkinson’s disease (PD). To develop a new technology to treat such diseases and disorders, especially PD, which is currently incurable, dopamine release from living cells intended for transplantation or drug screening must be precisely monitored and assessed. Owing to the advantages of miniaturisation and rapid detection, numerous electrical techniques have been reported, mostly in combination with various nanomaterials possessing specific nanoscale geometries. This review highlights recent advances in electrical biosensors for dopamine detection, with a particular focus on the use of various nanomaterials (e.g., carbon-based materials, hybrid gold nanostructures, metal oxides, and conductive polymers) on electrode surfaces to improve both sensor performance and biocompatibility. We conclude that this review will accelerate the development of electrical biosensors intended for the precise detection of metabolite release from living cells, which will ultimately lead to advances in therapeutic materials and techniques to cure various neurodegenerative disorders.
Management protocol for emergency aneurysm craniotomy clipping in non-major COVID-19 epidemic areas in Beijing, China
Authors: Chen Yu , Chen Xiaolin , Ma Li , Deng Xiaofeng , Li Zelin , Ye Xun , Wang Hao , Kang Shuai , Zhang Yan , Wang Rong , Zhang Dong , Cao Yong , Zhao Yuanli , Wang Shuo , Zhao Jizong 
Published: December 2020

Abstract
Background
An epidemic of COVID-19 broke out in Wuhan, China, since December 2019. The ordinary medical services were hindered. However, the emergency cases, including aneurysmal subarachnoid hemorrhage (aSAH), still required timely intervention. Thus, it provoked challenges to the routine management protocol. In this study, we summarized our experience in the emergency management of aSAH (Beijing Tiantan Protocol, BTP) in Beijing, China.

Methods
Demographic, clinical, and imaging data of consecutive emergency aSAH patients who underwent craniotomy clipping during the COVID-19 epidemic season were reviewed and compared with the retrospective period last year. Subgroup analysis was further performed to assess the outcomes of different screening results and several detailed protocols. Neurological outcomes were evaluated by the modified Rankin Scale (mRS).

Results
A total of 127 aSAH were referred to our emergency department, and 42 (33.1%) underwent craniotomy clipping between January 20, 2020, and March 25, 2020. The incidence of preoperative hospitalized adverse events and the perioperative outcomes were similar (− 0.1, 95% CI − 1.0 to 0.8, P = 0.779) to the retrospective period last year (January 2019–March 2019). After the propensity score matching (PSM), there were still no statistical differences in prognostic parameters between the two groups. Eight (19.0%) of the 42 individuals were initially screened as preliminary undetermined COVID-19 cases, in which 2 of them underwent craniotomy clipping in the negative pressure operating room (OR). The prognosis of patients with varied COVID-19 screening results was similar (F(2, 39) = 0.393, P = 0.678). Since February 28, 12 cases (28.6%) received COVID-19 nucleic acid testing (NAT) upon admission, and all showed negative. The false-negative rate was 0.0%. The preoperative hospitalized adverse events and postoperative prognosis were still similar between patients with and without COVID-19 NAT (− 0.3, 95% CI − 1.4 to 0.9, P = 0.653).

Conclusions
Our emergency surgery management protocol (BTP) is reliable for scheduling emergency aneurysm craniotomy clipping in non-major epidemic areas.
11C-UCB-J synaptic PET and multimodal imaging in dementia with Lewy bodies
Authors: Nicastro Nicolas , Holland Negin , Savulich George , Carter Stephen F. , Mak Elijah , Hong Young T. , Milicevic Sephton Selena , Fryer Tim D. , Aigbirhio Franklin I. , Rowe James B. , O’Brien John T. 
Published: December 2020

AbstractObjectiveDementia with Lewy bodies (DLB) is a common cause of dementia, but atrophy is mild compared to Alzheimer’s disease. We propose that DLB is associated instead with severe synaptic loss, and we test this hypothesis in vivo using positron emission tomography (PET) imaging of11C-UCB-J, a ligand for presynaptic vesicle protein 2A (SV2A), a vesicle membrane protein ubiquitously expressed in synapses.MethodsWe performed11C-UCB-J PET in two DLB patients (an amyloid-negative male and an amyloid-positive female in their 70s) and 10 similarly aged healthy controls. The DLB subjects also underwent PET imaging of amyloid (11C-PiB) and tau (18F-AV-1451).11C-UCB-J binding was quantified using non-displaceable binding potential (BPND) determined from dynamic imaging. Changes in11C-UCB-J binding were correlated with MRI regional brain volume,11C-PiB uptake and18F-AV-1451 binding.ResultsCompared to controls, both patients had decreased11C-UCB-J binding, especially in parietal and occipital regions (FDR-correctedp< 0.05). There were no significant correlations across regions between11C-UCB-J binding and grey matter, tau (18F-AV1451) or amyloid (11C-PiB) in either patient.ConclusionsQuantitative imaging of in vivo synaptic density in DLB is a promising approach to understanding the mechanisms of DLB, over and above changes in grey matter volume and concurrent amyloid/tau deposition.
Pesticide exposure and lung cancer risk: A case-control study in Nakhon Sawan, Thailand
Authors: Kangkhetkron Teera , Juntarawijit Chudchawal 
Published: December 2020

Background: Pesticide exposure might increase risk of lung cancer. The purpose of this study was to investigate the association between the historical use of pesticides commonly found in Thailand, and lung cancer. Methods: This case-control study compared a lifetime pesticide exposure of 233 lung cancer cases, and 447 healthy neighbours matched for gender, and age (±5 years). Data on demographic, pesticide exposure and other related factors were collected using a face-to-face interview questionnaire. Associations between lung cancer and types of pesticides as well as individual pesticides were analyzed using logistic regression adjusted for gender (male, female), age (≤54, 55-64, 65-74, ≥75), cigarette smoking ( never smoked, smoked < 109,500, smoked ≥ 109,500), occupation (farmer, non-farmer), cooking fumes exposure (yes, no), and exposure to air pollution (yes, no). Results: It was found that lung cancer was positively associated with lifetime use of herbicides, insecticides, and fungicides. Compared to people in the lowest quartile of number of days using the herbicides and insecticides, those in a higher quartile had an elevated risk of lung cancer, with odds ratio (OR) between 3.31 (95% confidence interval (CI) 1.49–7.34), and 12.58 (95% CI 5.70-27.75) (p < 0.001). For fungicides, only the most exposed group had a significant risk (OR = 4.25; 95% CI 1.23-14.72). For individual pesticides, those presenting a significant association with lung cancer were dieldrin (OR = 2.56; 95% CI 1.36-4.81), chlorpyrifos (OR = 3.29; 95 % CI 1.93-5.61), and carbofuran (OR = 2.10; 95% CI 1.28-3.42). Conclusions: The results showed that lung cancer among Thai people in Nakhon Sawan province is associated with previous pesticide use. This finding was consistent with previous studies in other parts of the world. Further study should focus on identifying more individual compounds that may cause lung cancer, as well as other types of cancer.
Cyclic stroke mortality variations follow sunspot patterns
Authors: Geronikolou Stella , Leontitsis Alexandros , Petropoulos Vasilis , Davos Constantinos , Cokkinos Dennis , Chrousos George 
Published: December 2020

Background: Mapping time-structures is a burgeoning scientific field enriching the (P4) medicine models. Local evidence in Mediterranean populations is underinvestigated. Methods: The Censused stroke-related death events (D) in the largest East-Mediterranean port (Piraeus), during (1985-1989), when local population had diet (low fat/sugar, proteins and vegetables/fruits daily, and pure olive oil almost exclusively) and genetic homogeneity-later interrupted by the immigration into Greece in 1990; and Sunspot numbers were indexed by Wolf numbers (Rz) (1944-2004), and evaluated using Fast Fourier Analysis and Singular Spectrum Analysis in MATLAB. Results: D were turned with fluctuations >35% in Rz. A non-anthropogenic 6.8 days cycle was recognized. Conclusions: This study may be taken into consideration in future public health planning and chronotherapy evaluations.
Oral health status of female hospitalized psychiatric patients in Jazan: A Case-control study
Authors: Alakhali Mohammed Sultan , Ibraheem Wael Ibraheem M , Al-Maweri Sadeq Ali , Al neami Salha Ibrahim , Alhuraysi Fatimah Mohammed 
Published: December 2020

Objectives: Poor oral health is an important determinant of general health in patients with mental illnesses. The present study was conducted to evaluate the dental and periodontal status of hospitalized female psychiatric patients in Jazan city, Saudi Arabia. Materials and Methods: This study included 82 subjects: 37 psychiatric female patients, and 45 age- and gender- matched healthy controls. Dental caries experience (decayed, missing, and filled teeth index ( DMFT ) and periodontal health status were recorded. SPSS version 21 was used for data analysis, and p-value < 0.05 was considered statistically significant. Results: The mean ages were comparable among the two groups. Psychiatric patients showed poor oral hygiene practices, with only 56.6% reported regularly brushing their teeth as compared with 82.2% of controls (P < 0.05). Control subjects had significantly higher DMFT mean scores than psychiatric female patients. However, psychiatric patients had significantly higher mean scores of missing teeth (M component) and lower restored teeth (F component) as compared with controls. Moreover, the mean attachment loss scores were significantly higher in the psychiatric group (0.76±1.58) compared with controls (0.23; 0.44 p < 0.01). Conclusions: Psychiatric patients showed poor oral hygiene practices and a high prevalence of untreated dental caries and periodontal diseases. Effective oral health promotion strategies need to be implemented to improve the oral health and oral health practices of this group of people.
Keywords
Psychiatric patients; Dental; Periodontal disease; Oral hygiene.
Artigo: Da Silveira TB, Tavella RA, Fernandez JB, Ribeiro APFA, Garcia EM, da Silva Júnior FMR. Perfil epidemiológico de recém-nascidos internados em Unidades de Terapia Intensiva Neonatal em hospitais universitários no extremo Sul do Brasil. Vittalle 2020; 32(2): 46-54.
Authors: Martins Thiago 
Published: December 2020

Como comentários ao artigo “Perfil epidemiológico de recém-nascidos internados em Unidades de Terapia Intensiva Neonatal em hospitais universitários no extremo Sul do Brasil.” (1) publicado na sua estimada revista (volume 32, número 2, 2020), gostaria de complementar alguns pontos.
O artigo demonstra que a prematuridade, baixo peso ao nascer, perímetro cefálico reduzido e doenças respiratórias foram os desfechos desfavoráveis mais prevalentes entre os recém nascidos internados (1). A prematuridade aumenta a prevalência da retinopatia da prematuridade que está entre as principais causas de cegueira prevenível nessa faixa etária (2,3). Isso demonstra a necessidade de capacitação adequada dos médicos para lidar com essas patologias. A formação de especialistas em retinopatia da prematuridade e muitas vezes inadequada, que muitas vezes discordam dos critérios clínicos utilizados na avaliação dos estágios da retinopatia da prematuridade (4).
Esses problemas podem ser solucionados com a melhor capacitação dos médicos generalistas na triagem de problemas oftalmológicos, como com o desenvolvimento de modelos de ensino para o treinamento do teste do reflexo vermelho (5). Outra alternativa é o desenvolvimento de novos equipamentos que auxiliem a captura da imagem da retina dos prematuros e permita o adequado acompanhamento desses pacientes por programas de telemedicina (6).
Outra solução para o aumento de demanda de patologias oftalmológicas em recém nascidos e o desenvolvimento de algoritmos de inteligência artificial que permitem uma análise automática das imagens, avaliando alguns graus de retinopatia da prematuridade, principalmente avaliando a forma plus da doença, que possui um aumento da tortuosidade vascular. Contudo, os algoritmos ainda possuem uma dificuldade de avaliar adequadamente as imagens da periferia da retina do recém-nascido, que são os locais de maior interesse, já que a vascularização da retina só atinge a região periférica com o adequado desenvolvimento desses pacientes (7)
Dessa forma, o conhecimento do perfil dos recém nascidos das unidades de terapia intensiva são importantes para o desenvolvimento de políticas públicas adequadas de triagem das patologias mais prevalentes. Essas políticas podem englobar uma adequada formação dos profissionais de saúde, além do uso da tecnologia como o desenvolvimento de programas de telemedicina ou inteligência artificial. O retorno desse investimento e garantido pela redução de importantes causas de cegueira preveníveis devido ao aumento do número de prematuros de risco.
Development and Validation of a Risk Prediction Model for Esophageal Squamous Cell Carcinoma Using Cohort Studies
Authors: Wang Qiao-Li , Ness-Jensen Eivind , Santoni Giola , Xie Shao-Hua , Lagergren Jesper 
Published: December 2020

INTRODUCTION:
Esophageal squamous cell carcinoma (ESCC) carries a poor prognosis, but earlier tumor detection would improve survival. We aimed to develop and externally validate a risk prediction model based on exposure to readily available risk factors to identify high-risk individuals of ESCC.

METHODS:
Competing risk regression modeling was used to develop a risk prediction model. Individuals' absolute risk of ESCC during follow-up was computed with the cumulative incidence function. We used prospectively collected data from the Nord-Trøndelag Health Study (HUNT) for model derivation and the UK Biobank cohort for validation. Candidate predictors were age, sex, tobacco smoking, alcohol consumption, body mass index (BMI), education, cohabitation, physical exercise, and employment. Model performance was validated internally and externally by evaluating model discrimination using the area under the receiver-operating characteristic curve (AUC) and model calibration.

RESULTS:
The developed risk prediction model included age, sex, smoking, alcohol, and BMI. The AUC for 5-year risk of ESCC was 0.76 (95% confidence interval [CI], 0.58–0.93) in the derivation cohort and 0.70 (95% CI, 0.64–0.75) in the validation cohort. The calibration showed close agreement between the predicted cumulative risk and observed probabilities of developing ESCC. Higher net benefit was observed when applying the risk prediction model than considering all participants as being at high risk, indicating good clinical usefulness. A web tool for risk calculation was developed: https://sites.google.com/view/escc-ugis-ki.

DISCUSSION:
This ESCC risk prediction model showed good discrimination and calibration and validated well in an independent cohort. This readily available model can help select high-risk individuals for preventive interventions.