bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maximizing the value of Korshinsk peashrub branches by the integration of Pleurotus tuoliensis cultivation and anaerobic digestion of spent mushroom substrate
Authors: Du Fang , Qu Jibin , Hu Qingxiu , Zou Yajie , Yin Guanhong , Dai Dari , Yuan Xufeng 
Published: March 2020

Abstract
Background: Korshinsk peashrub branches are agroforestry byproducts that accumulate in millions of tons per year and need to be rationally and efficiently utilized. To maximate the utilization value of Korshinsk peashrub branches, an integrated process was proposed and conducted by using Korshinsk peashrub branches as the main substrate ingredient for Pleurotus tuoliensis cultivation and performing anaerobic digestion of spent mushroom substrate for methane production in this study.Results: It was found that the substrate including 45% Korshinsk peashrub branches obtained higher mushroom yields than that using a commercial substrate, and anaerobic digestion of spent mushroom substrate from P. tuoliensis cultivation achieved a high cumulative methane yield. Preliminary calculation suggested that, for every kg of Korshinsk peashrub branches consumption, 1.12 kg mushroom product and 8578 L methane were produced, obtaining actual economic value of RMB 27.5 Yuan. In addition, this integrated stragety have positive ecological and social benefits.Conclusion: The value of Korshinsk peashrub branches can be maximized by P. tuoliensis cultivation and anaerobic digestion of spent mushroom substrate, which not only promote the biological conversion of Korshinsk peashrub branches to circle economy, but also be beneficial for the sustainable development of environment, energy conservation and edible fungi industry.
ITRAQ-based quantitative proteomic analysis of Fusarium moniliforme (Fusarium verticillioides) in response to Phloridzin inducers
Authors: Zhang Rong , Jiang Weitao , Liu Xin , Duan Yanan , Xiang Li , Wang Yanfang , Jiang Yuanmao , Shen Xiang , Chen Xuesen , Yin Chengmiao , Mao Zhiquan 
Published: March 2020

Abstract
Apple replant disease (ARD) has been reported from all major fruit-growing regions of the world, and is often caused by biotic factors (pathogen fungi) and abiotic factors (phenolic compounds). In order to clarify the proteomic differences of Fusarium moniliforme under the action of phloridzin, and to explore the potential mechanism of F. moniliforme as the pathogen of ARD, the role of Fusarium spp in ARD was further clarified. In this paper, the quantitative proteomics method iTRAQ analysis technology was used to analyze the proteomic differences of F. moniliforme before and after phloridzin treatment. A total of 4535 proteins were detected, and 293 proteins were found with more than 1.2 times (P<0.05) differences. In-depth data analysis revealed that differentially expressed proteins were influenced a variety of cellular processes, particularly metabolic processes. Among these metabolic pathways, a total of 8 significantly enriched KEGG pathways were identified with at least 2 affiliated proteins with different abundance in conidia and mycelium. Functional pathway analysis indicated that 46 up-regulated proteins were mainly distributed in amino sugar, nucleotide sugar metabolism, glycolysis/ gluconeogenesis and phagosome pathways. The results deepened our understanding of the proteome in F. moniliforme in response to phloridzin inducers and provide a basis for further exploration for improving the efficiency of the fungi as biocontrol agents to control ARD.
Patients' Preferences in Selecting Family Physician in Primary Health Centers: A Qualitative-Quantitative Approach
Authors: Khatami Farnaz , Shariati Mohammad , Khedmat Leila , Bahmani Maryam 
Published: March 2020

Abstract
Background The role of family physicians (FPs) in the metropolitan area is critical in identifying risk factors for diseaseprevention/controland health promotioninvarious age groups. Understanding people's preferences and interests in choosing a FP can be an effective and fundamental step in the success of this program. In this study factors affecting the FP selection by Iranian people referred to health centersin the most populous areas in the south of Tehran were assessed and ranked.Methods Asequential mixed-method (qualitative-quantitative) triangulation approach was designed with three subject groups of people, physicians, and health officials.The Framework method was used to analyze interviews transcribed verbatim. After implementing an iterative thematic process, a 26-item quantitative questionnaire with high validity and reliability was draftedto evaluatethe different factors.A convenient sampling method was usedto select 400 subjectson a population-based scale to quantitatively rank the most critical selection factors.Results The selection factors were divided into six centralized codes, including FPs' ethics,individual, professional and performance factors;patients underlying disease and individual health, and disease-related factors;clinic-office’s location and management factors;democracyfactors;economic factors; andsocial factors. After filling out the questionnaires,the most important factors in selecting FP were aspecialistdegree in family medicine (FM), performing accurate examinations, taking correct biography, and spending enough time to visit by the doctor, respectively. However, the parameters such as being a fellow-citizen, being same gender, and physician's appearance were of the least importance.Conclusion There is a necessity to expand the new medical specialty in FM because it was considered as the first people's priority. The clinical andadministrative healthcare systems should schedule the entire implementation process to oversee the doctor's professional commitment and setting the visit times of FP.
Analysis clinical features of COVID-19 infection in secondary epidemic area and report potential biomarkers in evaluation
Authors: Ji Weiping , Bishnu Gautam , Shen Xian , Cai Zhenzhai 
Published: March 2020

Abstract
Objective: Based on the clinical characteristics of infected patients with novel coronavirus in secondary epidemic areas, we aimed to identify potential biomarkers for the evaluation of novel coronavirus-infected patients, guide the diagnosis and treatment of this disease in secondary epidemic areas and provide a reference for the clinical prevention and control of this epidemic situation.Methods: The clinical data of 33 patients with respiratory symptoms caused by the novel coronavirus in Wenzhou city from January 15 to February 12, 2020, were thoroughly reviewed. At the onset of the disease, we found that the primary symptoms were fever, cough, fatigue, chest tightness, chest pain and specific blood test results. According to the patients’ histories, the patients were divided into two groups: those who spent time in the main epidemic area and those who did not spend time in the main epidemic area. The differences in the clinical manifestations between these two groups were analyzed.Results: The main clinical symptoms of patients infected with novel coronavirus in the secondary epidemic area were respiratory tract ailments and systemic symptoms. After grouping patients based on the presence or absence of residency in or travel history to the main epidemic area, there was no significant difference between the baseline data of these two groups, and there were no significant differences in symptoms and signs between the two groups (P>0.05). Some patients had abnormally increased serum amyloid protein A (SAA). There were statistically significant differences in the leukocyte count/C-reactive protein, monocyte ratio/C-reactive protein, neutrophil count/C-reactive protein, monocyte count/C-reactive protein and hemoglobin/C-reactive protein values between the two groups (P < 0.05).Conclusion: Respiratory tract ailments and systemic symptoms were the primary symptoms of novel coronavirus infection in the secondary epidemic area; these symptoms are not typical. The abnormal increase in serum amyloid protein (SAA) may be used as an auxiliary index for diagnosis and treatment. CRP changes before other blood parameters and thus may be an effective evaluation index for patients with COVID-19 infection.
Timing of optimal surgical intervention for vitreous hemorrhage in patients with proliferative diabetic retinopathy
Authors: Lin Li , Chen Yiqi , Wang Lin , Shen Lijun 
Published: March 2020

Abstract
Background：To explore the optimal timing of vitrectomy for vitreous hemorrhage in the patients with proliferative diabetic retinopathy (PDR).Method：Seventy-six PDR patients (85 eyes) who were diagnosed with vitreous hemorrhage and received vitrectomy were retrospectively enrolled into this study. The patients were categorized into 4 groups based on the timing of surgical intervention after the occurrence of hemorrhage, namely Group 1 (less than 15 days, with 28 eyes of 27 patients), Group 2 (15~30 days, with 29 eyes of 27 patients), Group 3 (30~90 days, with 14 eyes of 11 patients) and Group 4 (>90 days, with 14 eyes of 11 patients). Best corrected visual acuity (BCVA) and central macular thickness (CMT) from OCT before and at 1 mo and 3 mo after operation were collected as the main outcomes. The postoperative complications were also reported.Result: 1) BCVA (logMAR): the mean pre-operation BCVA was not significantly different among the 4 groups (P=0.139), while the mean post-operation BCVA was significantly worse in groups with longer gap between the vitreous bleeding and the vitrectomy, both at 1m and 3m after surgery (P=0.043; P<0.001). 2) CMT(μm): Although CMT was unavailable to measure in most included eyes before surgery, the mean CMT was significantly thicker in groups with postponed surgical intervention both at 1-month (P=0.001) and at 3-month (P=0.006) after surgery. 3) Complications: No severe complications were spotted in early vitrectomy groups (Group 1 and Group 2) during the follow-up period. At 3 months after the operation, three cases of ocular hypertension and another case of neovascular glaucoma in Group 3 and 4 cases of ocular hypertension and another 3 cases of neovascular glaucoma in Group 4 were observed. Conclusion: Early vitrectomy is more effective in improving BCVA and decreasing CMT in the PDR patients with vitreous hemorrhage. Moreover, the post-operation complications were spotted less in early vitrectomy groups. Thus we suggested that vitrectomy should be performed early and better within one month after the onset of vitreous hemorrhage in PDR patients.
USP18 promotes cell proliferation and suppressed apoptosis in cervical cancer cells via activating AKT signaling pathway
Authors: Diao Wenjing , Guo Qisang , Zhu Caiying , Song Yu , Feng Hua , Cao Yuankui , Du Ming , Chen Huifen 
Published: March 2020

Abstract
Background: The deubiquitinating (DUB) enzyme ubiquitin-specific protease 18 (USP18), also known as UBP43, is an ubiquitin-specific protease and has been linked to several human malignancies. However, the underlying function of USP18 remains unclear in human cervical cancer. In the current research, we aimed to analyze the role of USP18 and its signaling pathway in cervical cancer. Methods: Quantitative real time polymerase chain reaction (qRT-PCR) and immunohistochemical staining were performed to analyze USP18 levels in cervical cancer and adjacent-matched tissues. Moreover, RNA interference (RNAi) and lentiviral-mediated vector were performed to silence and overexpression of USP18 in cervical cancer cells. Further, Cell Counting Kit-8 (CCK-8) assay and Annexin V/PI staining were used to assess its biological function in cell proliferation and apoptosis respectively. Results: Present findings demonstrated that USP18 was overexpressed in cervical cancer specimens and cell lines. Silencing of USP18 in cervical cancer cell lines, SiHa and Caski, inhibited cell proliferation, while induced apoptosis and promoted the expression of cleaved caspase-3. On the contrary, USP18 overexpression showed reversed effects in Hela cells. Gene Set Enrichment Analysis suggested that USP18 was enriched in PI3K/AKT signaling pathway in cervical cancer. Hence, the PI3K/AKT inhibitor LY294002 was used to determine the relationship between USP18 and AKT in cervical cancer cells. Importantly, the PI3K/AKT inhibitor LY294002 deeply abolished the effects of USP18 overexpression in cervical cancer cells. Conclusions: The current study indicates USP18 was an oncogenic gene in cervical cancer. Our findings not only deepened the understanding the biological function of USP18 in the pathogenesis of cervical cancer but also provided novel insight for cervical cancer therapy. Trial registration: retrospectively registered.
Expression of membrane progesterone receptors in eutopic and ectopic endometrium of women with endometriosis
Authors: VAZQUEZ-MARTINEZ EDGAR RICARDO , Bello-Alvarez Claudia , Hermenegildo-Molina Ana Lorena , Solís-Paredes Mario , Parra-Hernandez Sandra , Cruz-Orozco Oliver , Silvestri-Tomassoni J Roberto , Escobar-Ponce Luis F , Hernandez-Lopez Luis A , Reyes-Mayoral Christian , Olguín-Ortega Andrea , Sánchez-Ramírez Brenda , Osorio-Caballero Mauricio , García-Gómez Elizabeth , Estrada-Gutiérrez Guadalupe , Cerbón Marco , Camacho-Arroyo Ignacio 
Published: March 2020

Abstract
Background: Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by non-genomic mechanisms via membrane progesterone receptors that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of PAQR family members in the eutopic and ectopic endometrium of women with endometriosis. Methods : Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 ( mPRα ) , PAQR8 , (mPRβ) PAQR5 ( mPRγ ) , and PAQR6 ( mPRδ ) at mRNA and protein levels was evaluated by RT-qPCR and western blot. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey's multiple comparisons test with a confidence interval of 95%. Results : The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Conclusions : Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease.
Genome-wide analysis of DNA methylation in hypertension-discordant monozygotic twins
Authors: Wu Zhentian , Gao Wenjing , Cao Weihua , Li Chunxiao , Yu Canqing , Lv Jun , Pang Zengchang , Cong Liming , Wang Hua , Wu Xianping , Li Liming 
Published: March 2020

Abstract
Background: DNA methylation has great potential for identifying the aetiology of hypertension. The aim of this study was to explore the correlation between hypertension and DNA methylation using twins discordant for hypertension in China. Methods: In this study, 43 pairs of monozygotic twins discordant for hypertension (age 31.9-72.3 years; 67.4% male) from the Chinese National Twin Registry were recruited. Genome-wide DNA methylation was measured using the Illumina Human methylation EPIC Beadchip in whole-blood-derived DNA. Standardized questionnaires were used to collect twin data on the following variables: age, gender, socioeconomic level, lifestyle factors (including smoking, alcohol drinking, vegetable intake, and physical activity). Blood pressure, height, weight, and other anthropometric indicators were obtained by physical examination. Empirical Bayes paired moderated t-test was utilized to compare the methylation data within twin pairs. Results: Four suspected significant methylation sites, cg00950476, cg08041400, cg26733338, and cg08580087 were identified. All of these four sites locate on known loci, which were LINC01252, BDP1, SYT1, and ODZ4, respectively. The main function includes transcriptional regulation, learning and cognitive, neurodevelopment. The significant sites were further replicated among two different replication population, the first replication population contained 38 hypertension concordant monozygotic twin pairs and 38 non-hypertension concordant monozygotic twin pairs matched in age, sex, region, and birth order, and the second replication group included 21 MZ twin pairs discordant for hypertension . None of them, however, were significant. The methylation variation in the above sites may influence blood pressure, independent of genetic and early-life environmental factors. Conclusions: This study found four suspected methylation sites correlated with hypertension. However, all four sites failed the replication analysis. More hypertension-discordant monozygotic twin pairs are needed to replicate these findings in the future to explore the stability of the results.
Spotlight on Consent: Lessons from the SPOTLIGHT Trial for Acute Intracerebral Hemorrhage
Authors: Fitzpatrick Tess , Shamy Michel , Dewar Brian , Spence Julie , Demchuk Andrew M. , Hill Michael D. , Flaherty Matthew L. , Gladstone David J. 
Published: March 2020

Abstract
Background SPOTLIGHT was a Canadian multicentre, placebo-controlled, randomized trial of emergency treatment with recombinant Factor VIIa for patients with acute intracerebral hemorrhage that enrolled a subset of eligible patients via deferral of consent. We investigated attitudes towards deferral of consent among participants and their legally-authorized representatives (LARs). Methods All participants or LARs approached for enrolment in SPOTLIGHT were invited to complete an 11-item questionnaire within the first 4 days of enrolment, and again at 90 days. Results Eight out of 50 participants in SPOTLIGHT (16%) were enrolled via deferral of consent. Ten LARs for participants (20%) completed the initial survey and 6 completed the 90 day follow-up survey. Ninety percent of respondents agreed with the process of deferral of consent both in principle and specifically for the SPOTLIGHT trial. Participants were more likely to support deferral of consent for low-risk or time-sensitive interventions, or in situations with no alternative treatment options. Conclusions The majority of respondents were supportive of using deferral of consent to enrol participants into SPOTLIGHT and acute stroke trials.
Association between nonalcoholic fatty liver disease and extrahepatic cancers: a systematic review and meta-analysis
Authors: Liu Shou-Sheng , Ma Xue-Feng , Zhao Jie , Du Shui-Xian , Zhang Jie , Dong Meng-Zhen , Xin Yong-Ning 
Published: March 2020

Abstract
Background NAFLD was tightly associated with various diseases such as diabetes, cardiovascular disease, kidney disease and cancer. Previous studies had investigated the association between NAFLD and various extrahepatic cancers, but the conclusions were remains to be improved. The aim of this study was to investigate the association between NAFLD and various extrahepatic cancer comprehensively. Methods Electronic databases PubMed, EMBASE, Medline, and the Cochrane Library were searched for observational studies published from 1996 to January 2020. Observational studies that reflected the association between NAFLD and extrahepatic cancers were included in this study. The pooled OR/HR/IRR of the association between NAFLD and various extrahepatic cancers were analyzed. Results A total of 26 studies were included for investigating the association between NAFLD and various extrahepatic cancers. As the result shown, the pooled OR values of the risk of colorectal cancer and adenomas in patients with NAFLD were 1.72 (95%CI: 1.40-2.11) and 1.38 (95%CI: 1.22-1.56), respectively. The pooled OR values of the risk of intrahepatic cholangiocarcinoma and extrahepatic cholangiocarcinoma in patients with NAFLD were 2.40 (95%CI: 1.75-3.31) and 2.24 (95%CI: 1.58-3.17), respectively. The pooled OR value of the risk of breast cancer in patients with NAFLD was 1.68 (95%CI: 1.44-1.97). In addition, NAFLD was also tightly associatied with the risk of gastric cancer, pancreas cancer, prostate cancer, and esophagus cancer. Conclusions NAFLD could significantly increase the development risk of colorectal adenomas and cancer, intrahepatic and extrahepatic cholangiocarcinoma, breast cancer, gastric cancer, pancreas cancer, prostate cancer, and esophagus cancer.
Sex-based difference in influence of A blood group on increased risk of coronary artery disease among Chinese adults: a propensity score matching analysis
Authors: Song Yanbin , Cai Gaojun , Yang Qichao , Li Feng , Lu Wei 
Published: March 2020

Abstract
Background

ABO blood group is a risk factor for coronary artery disease (CAD), but whether it is more strongly associated with CAD in Chinese individuals is less clear. The aim of this current study was therefore to investigate the association among patients hospitalized for primary coronary angiography and to explore the potential mediator of plasma lipids.

Methods

A total of 1079 patients, including 698 with- and 381 without newly diagnosed CAD, were retrospectively enrolled from November 2012 to December 2018. Clinical characteristics, plasma lipid levels and ABO blood groups were recorded and analyzed. Characteristics of the patients with and without CAD were compared after propensity score matching analysis (346 vs. 346). Correlation analysis was performed to assess the relationship between ABO types and clinical variables. Logistic regression analysis was constructed to determine the association of ABO blood group with CAD.

Results

There was not significant difference in the distribution of ABO types between CAD and non-CAD group in overall population. However, propensity-matched analysis showed that CAD in the women subgroup presented more frequently with blood group A, but not non-CAD (60.2% versus 44.1%, P =0.014). The statistically significant difference was not observed among men (37.5% versus 30.3%, P =0.157). When compared with non-A blood group, the A blood group had not a greater difference in lipid profiles among women ( P all>0.05). Correlation analysis performed in women revealed that CAD was significantly correlated with A type (r= 0.171, P = 0.003). After adjustment for age, hypertension, cigarette smoking, drinking and lipid profiles, logistic regression analysis showed that blood group A was an significant predictor for CAD in women (OR=2.152, 95% CI 1.285~3.605, P =0.004) .

Conclusion

Cumulatively, the present study indicated that blood group A was a significantly risk factor for coronary artery disease in Chinese women.