bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cyclical aggregation extends in vitro expansion potential of human mesenchymal stem cells
Authors: Bijonowski Brent M. , Yuan Xuegang , Jeske Richard , Li Yan , Grant Samuel C. 
Published: November 2020

AbstractMesenchymal stem cell (MSC)-based therapy has shown great promises in various animal disease models. However, this therapeutic potency has not been well claimed when applied to human clinical trials. This is due to both the availability of MSCs at the time of administration and lack of viable expansion strategies. MSCs are very susceptible to in vitro culture environment and tend to adapt the microenvironment which could lead to cellular senescence and aging. Therefore, extended in vitro expansion induces loss of MSC functionality and its clinical relevance. To combat this effect, this work assessed a novel cyclical aggregation as a means of expanding MSCs to maintain stem cell functionality. The cyclical aggregation consists of an aggregation phase and an expansion phase by replating the dissociated MSC aggregates onto planar tissue culture surfaces. The results indicate that cyclical aggregation maintains proliferative capability, stem cell proteins, and clonogenicity, and prevents the acquisition of senescence. To determine why aggregation was responsible for this phenomenon, the integrated stress response pathway was probed with salubrial and GSK-2606414. Treatment with salubrial had no significant effect, while GSK-2606414 mitigated the effects of aggregation leading to in vitro aging. This method holds the potential to increase the clinical relevance of MSC therapeutic effects from small model systems (such as rats and mice) to humans, and may open the potential of patient-derived MSCs for treatment thereby removing the need for immunosuppression.
The microbiota of farmed mink (Neovison vison) follows a successional development and is affected by early life antibiotic exposure
Authors: Bahl Martin Iain , Honoré Anabelle Legarth , Skønager Sanne Tygesen , Honoré Oliver Legarth , Clausen Tove , Andresen Lars , Hammer Anne Sofie 
Published: November 2020

AbstractOn many mink farms, antibiotics are used extensively during the lactation period to reduce the prevalence and severity of pre-weaning diarrhoea (PWD) in mink kits (also referred to as greasy kit syndrome). Concerns have been raised, that routine treatment of PWD with antibiotics could affect the natural successional development of the gut microbiota, which may have long lasting consequences. Here we investigated the effects of early life antibiotic treatment administered for 1 week (postnatal days 13–20). Two routes of antibiotic administration were compared to a non-treated control group (CTR, n = 24). Routes of administration included indirect treatment, through the milk from dams receiving antibiotics by intramuscular administration (ABX_D, n = 24) and direct treatment by intramuscular administration to the kits (ABX_K, n = 24). A tendency for slightly increased weight at termination (Day 205) was observed in the ABX_K group. The gut microbiota composition was profiled by 16S rRNA gene sequencing at eight time points between Day 7 and Day 205. A clear successional development of the gut microbiota composition was observed and both treatment regimens caused detectable changes in the gut microbiota until at least eight days after treatment ceased. At termination, a significant positive correlation was identified between microbial diversity and animal weight.
Genetic risk score associations for myocardial infarction are comparable in persons with and without rheumatoid arthritis: the population-based HUNT study
Authors: Rostami S. , Hoff M. , Dalen H. , Hveem K. , Videm V. 
Published: November 2020

AbstractPersons with rheumatoid arthritis (RA) have increased risk of myocardial infarction (MI). Overlapping associations with MI of weighted genetic risk scores (wGRS) for coronary artery disease (CAD) and RA is unknown in a population-based setting. Data from the prospective Nord-Trøndelag Health Study (HUNT2: 1995–1997 and HUNT3: 2006–2008) were used. wGRS added each participant’s carriage of all risk variants weighted by the coefficient from published association studies. Published wGRS for CAD and RA were analysed in Cox regression with MI as outcome, age as analysis time, and censoring at the first MI, death, or 31.12.2017. 2609 of 61,465 participants developed MI during follow-up (mean 17.7 years). The best-fitting wGRS for CAD and RA included 157 and 27 single-nucleotide polymorphisms, respectively. In multivariable analysis including traditional CAD risk factors, the CAD wGRS was associated with MI [hazard ratio = 1.23 (95% CI 1.18–1.27) for each SD increase, p < 0.0001] in RA patients (n = 433) and controls. The RA wGRS was not significant (p = 0.06). Independently from traditional risk factors, a CAD wGRS was significantly associated with the risk for MI in RA patients and controls, whereas an RA wGRS was not. The captured genetic risk for RA contributed little to the risk of MI.
Complex fibroblast response to glucocorticoids may underlie variability of clinical efficacy in the vocal folds
Authors: Nakamura Ryosuke , Mukudai Shigeyuki , Bing Renjie , Garabedian Michael J. , Branski Ryan C. 
Published: November 2020

AbstractSimilar to the hypertrophic scar and keloids, the efficacy of glucorticoids (GC) for vocal fold injury is highly variable. We previously reported dexamethasone enhanced the pro-fibrotic effects of transforming growth factor (TGF)-β as a potential mechanism for inconsistent clinical outcomes. In the current study, we sought to determine the mechanism(s) whereby GCs influence the fibrotic response and mechanisms underlying these effects with an emphasis on TGF-β and nuclear receptor subfamily 4 group A member 1 (NR4A1) signaling. Human VF fibroblasts (HVOX) were treated with three commonly-employed GCs+ /-TGF-β1. Phosphorylation of the glucocorticoid receptor (GR:NR3C1) and activation of NR4A1 was analyzed by western blotting. Genes involved in the fibrotic response, including ACTA2, TGFBR1, and TGFBR2 were analyzed by qPCR. RNA-seq was performed to identify global changes in gene expression induced by dexamethasone. GCs enhanced phosphorylation of GR at Ser211 and TGF-β-induced ACTA2 expression. Dexamethasone upregulated TGFBR1, and TGFBR2 in the presence of TGF-β1 and increased active NR4A1. RNA-seq results confirmed numerous pathways, including TGF-β signaling, affected by dexamethasone. Synergistic pro-fibrotic effects of TGF-β were observed across GCs and appeared to be mediated, at least partially, via upregulation of TGF-β receptors. Dexamethasone exhibited diverse regulation of gene expression including NR4A1 upregulation consistent with the anti-fibrotic potential of GCs.
The electrochemical reaction controlled optical response of cholestrol oxidase (COx) conjugated CdSe/ZnS quantum dots
Authors: Arshad Humaira , Chaudhry Madeeha , Mehmood Shahid , Farooq Ayesha , Wang Minqiang , Bhatti A. S. 
Published: November 2020

AbstractThis paper reports the enhanced performance of cholesterol oxidase (COx) conjugated CdSe/ZnS quantum dots (QDs) by using water-soluble mercaptoacitic acid (MAA) as linker. The functionalized MAA-CdSe/ZnS QDs conjugated in four different dilutions of cholesterol oxidase significantly affected QDs photoluminescence intensities, which affected the process of charge transfer from QDs to MAA. The conjugation of COx to MAA-QDs in increased dilutions resulted in the regain of PL intensities, which were attributed to the passivation of MAA HOMO/LUMO states. The electrochemical impedance spectroscopy and cyclic voltammetry of the conjugated QDs were performed to get study the charge transfer mechanism. The 1:1000 diluted COx conjugated MAA-CdSe/ZnS QDs was found to have the lowest charge transfer resistance of 228 Ω, the highest diffusion (~ 1.39 × 10–13cm2/s) and charge transfer rates (~ 4.5 × 10–6 s−1) between the electrode and the redox species. The current study demonstrated the sensitivity of electrochemical and optical based detection on the alkaline.
Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
Authors: Moldován Norbert , Torma Gábor , Gulyás Gábor , Hornyák Ákos , Zádori Zoltán , Jefferson Victoria A. , Csabai Zsolt , Boldogkői Miklós , Tombácz Dóra , Meyer Florencia , Boldogkői Zsolt 
Published: November 2020

AbstractLong-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.
Reproductive inhibition among nestmate queens in the invasive Argentine ant
Authors: Abril Sílvia , Gómez Crisanto 
Published: November 2020

AbstractIn social species, the presence of several reproductive individuals can generate conflict. In social insects, as queen number increases, individual oviposition rate may decrease because of direct and indirect behavioural and/or chemical interactions. Understanding the factors that mediate differences in queen fecundity should provide insight into the regulation and maintenance of highly polygynous insect societies, such as those of the invasive Argentine ant (Linepithema humile). In this study, we investigated (1) whether differences in the oviposition rates of Argentine ant queens exposed to polygynous conditions could result from interactions among them; (2) whether such differences in fecundity stemmed from differences in worker attention; and (3) whether polygynous conditions affected the cuticular hydrocarbon profiles of queens (CHCs). We found that differences in queen fecundity and CHC profiles observed under polygynous conditions disappeared when queens were exposed to monogynous conditions, suggesting some form of reproductive inhibition may exist when queens cohabit. These differences did not seem to arise from variation in worker attention because more fecund queens were not more attractive to workers. Levels of some CHCs were higher in more fecund queens. These CHCs are associated with greater queen productivity and survival. Our findings indicate that such compounds could be multifunctional queen pheromones.
An integrative atlas of chicken long non-coding genes and their annotations across 25 tissues
Authors: Jehl Frédéric , Muret Kévin , Bernard Maria , Boutin Morgane , Lagoutte Laetitia , Désert Colette , Dehais Patrice , Esquerré Diane , Acloque Hervé , Giuffra Elisabetta , Djebali Sarah , Foissac Sylvain , Derrien Thomas , Pitel Frédérique , Zerjal Tatiana , Klopp Christophe , Lagarrigue Sandrine 
Published: November 2020

AbstractLong non-coding RNAs (LNC) regulate numerous biological processes. In contrast to human, the identification of LNC in farm species, like chicken, is still lacunar. We propose a catalogue of 52,075 chicken genes enriched in LNC (http://www.fragencode.org/), built from the Ensembl reference extended using novel LNC modelled here from 364 RNA-seq and LNC from four public databases. The Ensembl reference grew from 4,643 to 30,084 LNC, of which 59% and 41% with expression ≥ 0.5 and ≥ 1 TPM respectively. Characterization of these LNC relatively to the closest protein coding genes (PCG) revealed that 79% of LNC are in intergenic regions, as in other species. Expression analysis across 25 tissues revealed an enrichment of co-expressed LNC:PCG pairs, suggesting co-regulation and/or co-function. As expected LNC were more tissue-specific than PCG (25% vs. 10%). Similarly to human, 16% of chicken LNC hosted one or more miRNA. We highlighted a new chicken LNC, hosting miR155, conserved in human, highly expressed in immune tissues like miR155, and correlated with immunity-related PCG in both species. Among LNC:PCG pairs tissue-specific in the same tissue, we revealed an enrichment of divergent pairs with the PCG coding transcription factors, as for example LHX5, HXD3 and TBX4, in both human and chicken.
Modulation of granulosa cell function via CRISPR-Cas fuelled editing of BMPR-IB gene in goats (Capra hircus)
Authors: Kumar Sai , Punetha Meeti , Jose Bosco , Bharati Jaya , Khanna Shivani , Sonwane Arvind , Green Jonathan A. , Whitworth Kristin , Sarkar Mihir 
Published: November 2020

AbstractBMPs are multifunctional growth factors implicated in regulating the ovarian function as key intra-ovarian factors. Biological effects of BMPs are mediated through binding with membrane bound receptors like BMPR-IB and initiating downstream Smad signaling pathway. FecB mutation, regarded as a loss of function mutation in the BMPR-IB gene was identified in certain sheep breeds having high fecundity. Similar type of fecundity genes in goats have not been discovered so far. Hence, the current study was designed to investigate the effects of BMPR-IB gene modulation on granulosa cell function in goats. The BMPR-IB gene was knocked out using CRISPR-Cas technology in granulosa cells and cultured in vitro with BMP-4 stimulation for three different durations In addition, the FecB mutation was introduced in the BMPR-IB gene applying Easi-CRISPR followed by BMP-4/7 stimulation for 72 h. Steroidogenesis and cell viability were studied to explore the granulosa cell function on BMPR-IB gene modulation. BMPRs were found to be expressed stage specifically in granulosa cells of goats. Higher transcriptional abundance of R-Smads, LHR and FSHR indicating sensitisation of Smad signaling and increased gonadotropin sensitivity along with a significant reduction in the cell proliferation and viability was observed in granulosa cells upon BMPR-IB modulation. The inhibitory action of BMP-4/7 on P4 secretion was abolished in both KO and KI cells. Altogether, the study has revealed an altered Smad signaling, steroidogenesis and cell viability upon modulation of BMPR-IB gene in granulosa cells similar to that are documented in sheep breeds carrying the FecB mutation.
Genotyping and zoonotic potential of Enterocytozoon bieneusi in cattle farmed in Hainan Province, the southernmost region of China
Authors: Zheng Xin-Li , Zhou Huan-Huan , Ren Gangxu , Ma Tian-Ming , Cao Zong-Xi , Wei Li-Min , Liu Quan-Wei , Wang Feng , Zhang Yan , Liu Hai-Long , Xing Man-Ping , Huang Li-Li , Chao Zhe , Lu Gang 
Published: November 2020

Enterocytozoon bieneusi is an intestinal pathogen that infects a wide range of species, including humans. Cattle constitute an important host for E. bieneusi; however, there is a scarcity of information on the prevalence and genotyping of E. bieneusi in cattle in the Hainan Province of China. In this study, PCR analysis of 314 fecal samples from cattle in six cities of Hainan was performed for genotype identification. The average prevalence of E. bieneusi in these animals was 9.9% (31/314), and ranged from 0.0% (0/12) to 20.5% (8/39). Five known genotypes – EbpC (n = 14), BEB4 (n = 12), J (n = 2), I (n = 1), and CHG5 (n = 1) – and a novel genotype: HNC-I (n = 1) – were identified. Genotypes EbpC and HNC-I were placed in zoonotic Group 1, and the remaining four genotypes (BEB4, J, I, and CHG5) were placed in Group 2. Since 93.5% of the genotypes found in the cattle (29/31) (EbpC, BEB4, J, and I) have previously been found in humans, these genotypes are probably involved in the transmission of microsporidiosis to humans.
Wirksamkeit von Sprachtherapie bei Kindern mit UESS unter Berücksichtigung ihres Bullying- und Viktimisierungsverhaltens und dem mütterlichen Belastungsempfinden
Authors: Rennecke Lisa , Stumper Barbara , Petermann Franz 
Published: November 2020

ZusammenfassungDie vorliegende Studie untersucht die Wirkung von Therapieangeboten im ambulanten und stationären Behandlungssetting auf die expressiven Wortschatzleistungen und das Bullying- und Viktimisierungsverhalten von Kindern mit Sprachentwicklungsstörungen sowie das mütterliche Belastungsempfinden innerhalb eines Jahres. Die Ergebnisse deuten auf Entwicklungsvorteile der Kinder im stationären Setting in Bezug auf die expressive Wortschatzleistung sowie das Bullying- und Viktimisierungsverhalten hin.
Tränenwegsstenosen im Erwachsenenalter
Authors: Heichel Jens , Struck Hans-Gert 
Published: November 2020

ZusammenfassungZur Aufrechterhaltung der okulären Oberflächenhomöostase und damit den Erhalt der
optischen Eigenschaften sind Tränenproduktion, -verteilung und -abtransport
essenzielle physiologische Funktionen. Störungen dieser funktionellen Einheit
gehen mit starken Beschwerden und einem hohen Leidensdruck bei den Patienten
einher. Da etwa 3% unserer Patienten eine Tränenabflussstörung aufweisen, ist
die Thematik von hohem klinischem Interesse.
Netzhautablösung – Teil 1
Authors: Schick Tina , Heimann Heinrich , Schaub Friederike 
Published: November 2020

ZusammenfassungDieser Beitrag beinhaltet eine Übersicht über die Epidemiologie, die Risikofaktoren und das klinische Bild sowie die Diagnostik der rhegmatogenen (rissbedingten) Netzhautablösung. Diese ist von einer Retinoschisis sowie den traktiven und exsudativen Formen der Netzhautablösung hinsichtlich ihrer Häufigkeit, den Risikofaktoren, ihrer Entstehung und dem klinischen Bild zu unterscheiden.
Verringerung vollstationärer Behandlungstage in psychiatrischen Kliniken mit Modellvorhaben zur patientenzentrierten Versorgung mit globalem Budget (§ 64b SGB V)
Authors: Neumann Anne , Baum Fabian , Seifert Martin , Schoffer Olaf , Kliemt Roman , March Stefanie , Häckl Dennis , Swart Enno , Pfennig Andrea , Schmitt Jochen 
Published: November 2020

Zusammenfassung
Ziel der Studie Es wird die Entwicklung voll- und teilstationärer Behandlungsdauer sowie der PIA-Kontakte von Modellvorhaben nach § 64b SGB V über einen Nachbeobachtungszeitraum von 3 Jahren untersucht.
Methodik Patienten mit Referenzfall im ersten Modelljahr und einem Nachbeobachtungszeitraum von 3 Jahren wurden für 12 Modellvorhaben untersucht und mit gematchten Patienten der Regelversorgung verglichen.
Ergebnisse Analysiert wurden 11 536 Patienten. Im ersten Jahr nach Referenzfall war die Anzahl vollstationärer Tage in fast allen Kliniken mit Modellvorhaben geringer und die Anzahl teilstationärer Tage höher im Vergleich zur Regelversorgung. Diese Effekte verstärkten sich in Modellkliniken mit Vorläufervertrag. Die PIA-Inanspruchnahme war zwischen Modellvorhaben unterschiedlich ausgeprägt.
Schlussfolgerung Diese Analyse leistet einen Beitrag für evidenzgeleitete gesundheitspolitische Entscheidungen zur Eignung patientenzentrierter Versorgungskonzepte mit globalem Budget für die Regelversorgung.
Herz schlägt Hirn
Authors: Hertlein Margit 
Published: November 2020

Zusammenfassung2005 gab Steve Jobs in einer Abschlussrede für Stanford-Absolventen den Tipp: Folgt im Leben „Herz und Intuition“. Denn irgendwie wüssten die beiden schon, wo es langgeht. Ernsthaft jetzt? Ja, leider! Mit diesem Zitat wurde die Intuition, das Bauchgefühl oder der Strudelwurm für viele zum höchsten Entscheidungsorgan.
Interaktive Gesundheitskompetenz, soziodemografische Faktoren und der Zeitraum bis zur Inanspruchnahme psychotherapeutischer oder medikamentöser Behandlung – gibt es Hinweise auf soziale Ungleichheiten in der Therapie der Sozialen Angststörung?
Authors: Ernstmann Nicole , Heuser Christian , Halbach Sarah , Wegener Ingo , Rambau Stefanie , Forstner Andreas J. , Schumacher Johannes , Mücke Martin , Geiser Franziska , Conrad Rupert 
Published: November 2020

Zusammenfassung
Ziel Ziel ist die Untersuchung des Zusammenhangs zwischen soziodemografischen Merkmalen sowie der interaktiven Gesundheitskompetenz und dem Zeitraum zwischen der Behandlungsentscheidung und der Inanspruchnahme einer Therapie der Sozialen Angststörung.
Methodik Hierzu wurde eine Online-Befragung von N = 311 Personen mit Sozialer Angststörung durchgeführt (Responserate 54,1 %). Neben deskriptiven statistischen Verfahren wurde eine logistische Regressionsanalyse durchgeführt.
Ergebnisse Die Befragten sind durchschnittlich 46 Jahre alt (20–81), 59 % sind Frauen. Höheres Alter (OR 2,579), nicht in Partnerschaft lebend (OR 1,963), Angst vor persönlichen Kontakten (OR 5,716) und geringe (OR 3,585) bzw. mäßige (OR 3,144) interaktive Gesundheitskompetenz sind signifikant mit der Wartezeit auf eine Behandlung assoziiert.
Schlussfolgerung Die Ergebnisse können als Hinweise auf soziale Ungleichheiten in Bezug auf die Inanspruchnahme einer Therapie bei Personen mit sozialer Angststörung gedeutet werden.
Surgical and Protocolized Management of Distal Radius Nonunion
Authors: Pardo García Juan María , Jiménez Díaz Verónica , Barberia Biurrun Amaya , García Lamas Lorena , Porras Moreno Miguel , Cecilia López David 
Published: November 2020

Abstract
Introduction Nonunion of the distal radius is an extremely rare and serious complication with unpredictable outcomes. The aim of the present study is to analyze the radiological and functional results after a protocolized surgical treatment.
Material and Methods A retrospective review of case series of patients with diagnosis of nonunion of the distal radius surgically treated from 2010 to 2016. The diagnosis of nonunion was made at 6 months without signs of consolidation. According to computed tomography (CT) scan images, we propose a classification of this complication as type I: no deformity or mild deformity, with subchondral metaphyseal bone stock beneath lunate facet greater than 10 mm, with no degenerative changes at the distal radioulnar joint (DRUJ); type II: moderate deformity or degenerative changes in the DRUJ, distinguishing between IIA and IIB based on the subchondral metaphyseal bone stock; and type III: degenerative changes at the midcarpal joint. In all cases, a reconstructive technique consisting of volar locking plate osteosynthesis with autologous tailored iliac crest graft was performed, hence none of the cases were classified as type III.
Results In total, six cases were included. Mean follow-up: 58 months (range: 30–108 months). Consolidation was achieved in a mean period of 5 months (range: 3–9 months). Average active range of motion: flexion: 54° (range: 10°–80°); extension: 40° (range: 10°–85°); supination: 82.5° (range: 75°–85°); pronation 80° (range: 55–85°); radial deviation: 15° (range: 5°–20°); and ulnar deviation 15° (range: 5–20°). Mean strength: 24.15 Kg (6.9–35 Kg). The average score on the Visual Analog Scale (VAS) at rest was of 0.6 (0–2), and with movement, it was of 1.8 (0–4). The mean postoperative score on the Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire was of 18.48 (range: 0–50.8). Average radial height: 9.65 mm (range: 7–12 mm); average radial angulation: 17° (10°–20°); volar tilt: 8.36° (range: 0.2°–21°); average ulnar variance: 1.52 mm (range: 0–3.5 mm). Complications: persistent nonunion, superficial wound infection, acquired residual ulnar club hand deformity, radiocarpal arthritis, and midcarpal arthritis. Nevertheless, no salvage techniques were needed or requested during the follow-up, with all patients in an active work situation or living an active life in complete normality.
Conclusions The protocolized surgical treatment of pseudoarthrosis of the the distal radius, based on our proposed classification, offers good functional outcomes in the long-term follow-up.