bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histamine receptor 1 is expressed in leukaemic cells and affects differentiation sensitivity
Authors: Cornet‐Masana Josep M. , Banús‐Mulet Antònia , Cuesta‐Casanovas Laia , Carbó José M. , Guijarro Francesca , Torrente Miguel Ángel , Esteve Jordi , Risueño Ruth M. 
Published: October 2020

AbstractDespite the success of immunotherapy in several haematological neoplasms, the effectiveness in acute myeloid leukaemia (AML) is still controversial, partially due to the lack of knowledge regarding immune‐related processes in this disease and similar neoplasias. In this study, we analysed the role and expression of histamine receptor 1 (HRH1) in haematological malignancies. Although the histamine receptor type 1 was widely expressed in healthy and malignant haematopoiesis, especially along the myeloid lineage, HRH1 lacked a relevant role in survival/proliferation and chemoresistance of AML cells, as analysed by HRH1 knockdown (KD) and pharmacological modulation. However, HRH1‐mediated signalling was critical for the activation of the differentiation process induced by several agents including all‐trans retinoic acid, establishing a role for HRH1 in myeloid differentiation. Pharmacological activation of Erk was able to partially restore differentiation capacity in HRH1 KD AML cells, suggesting that HRH1 signalling acts upstream MAPK‐Erk pathway. As an indirect consequence of our results, treatment‐related histamine release is not expected to confer a proliferative advantage in leukaemic cells.
Treatment with interleukin‐33 is non‐toxic and protects retinal pigment epithelium in an ageing model of outer retinal degeneration
Authors: Clare Alison J. , Copland David A. , Nicholson Lindsay B. , Liu Jian , Neal Chris R. , Moss Stephen , Dick Andrew D. , Theodoropoulou Sofia 
Published: October 2020

AbstractThe leading cause of central vision loss, age‐related macular degeneration (AMD), is a degenerative disorder characterized by atrophy of retinal pigment epithelium (RPE) and photoreceptors. For 15% of cases, neovascularization occurs, leading to acute vision loss if left untreated. For the remaining patients, there are currently no treatment options and preventing progressive RPE atrophy remains the main therapeutic goal. Previously, we have shown treatment with interleukin‐33 can reduce choroidal neovascularization and attenuate tissue remodelling. Here, we investigate IL‐33 delivery in aged, high‐fat diet (HFD) fed mice on a wildtype and complement factor H heterozygous knockout background. We characterize the non‐toxic effect following intravitreal injection of IL‐33 and further demonstrate protective effects against RPE cell death with evidence of maintaining metabolic retinal homeostasis of Cfh+/−~HFD mice. Our results further support the potential utility of IL‐33 to prevent AMD progression.
The olfactory responses of mirid bugs to six plant extracts and their volatiles
Authors: Yin Haichen , Li Wenjing , Xu Min , Xu Dong , Wan Peng 
Published: October 2020

AbstractMany methods for management of mirid bugs, one of the major pest species in cotton fields, have been developed in recent decades. The use of attractive plant volatiles may be a safe and effective alternative compared with chemical pesticides. Very little is known about the responses of the bugs to some specific volatiles. In this study, the attractive effects for the mirid bug Apolygus lucorum were evaluated and compared among ethanol extracts obtained from six types of plants. The results showed that A. lucorum had the highest selection response rate to the extract from Allium tuberosum seeds and 70.51% of the adults tended to choose this odour source. Among 40 identified compounds of the volatiles comprising A. tuberosum seed extracts, diethyl phthalate and methyl levulinate had significantly higher attractive effects than that of cotton leaves, but the respective selection response rates were less than 65%. We found that when methyl levulinate and diethyl phthalate were mixed in volume ratios of 3:7 and 6:4, the selection response rates of A. lucorum for the mixtures were 54.67% and 63.65%, respectively. These response rates were significantly higher than those for the two compounds presented alone. Furthermore, the selection response rates of A. lucorum based on these mixtures reached 82.75% and 75.73%, respectively when compared with cotton leaves. The two volatile mixtures also showed a significantly higher attractive effect for Adelphocoris suturalis, with respective selection response rates at 74.58% and 77.80% when compared with cotton leaves. Moreover, the two attractants showed no difference between the responses of male and female mirid bugs. We concluded that an appropriate application of the attractants described herein has the potential to develop a safe and effective approach for the monitoring and control of mirid bugs in cotton fields.
Cytochrome oxidase gene sequencing reveals channel catfish ovary cell line is contaminated with brown bullhead cells
Authors: Ford Lorelei , Subramaniam Kuttichantran , Waltzek Thomas B. , Bowser Paul R. , Hanson Larry 
Published: October 2020

AbstractThe channel catfish (Ictalurus punctatus, Rafinesque) ovary (CCO) cell line is the standard cell line used for channel catfish diagnostics. Next‐gen sequencing studies of a virus cultured in the CCO cells revealed mitochondrial sequences matching those of brown bullhead (Ameiurus nebulosus, Lesueur). Therefore, we systematically performed partial cytochrome oxidase 1 gene sequencing of several sources of the CCO cell line and all matched the brown bullhead and not the channel catfish.
Prevention of post‐endoscopic retrograde cholangiopancreatography pancreatitis with a combination of pharmacological agents based on rectal non‐steroidal anti‐inflammatory drugs: A systematic review and network meta‐analysis
Authors: Oh Hyoung‐Chul , Kang Hyun , Park Tae Young , Choi Geun Joo , Lehman Glen A 
Published: October 2020

AbstractBackground and AimsRectally administered non‐steroidal anti‐inflammatory drugs (NSAIDs) are effective but suboptimal in the prevention of post‐endoscopic retrograde cholangiopancreatography pancreatitis or PEP. New trials with the combination of rectal NSAIDs and other pharmacological agents have been conducted. This network meta‐analysis (NMA) aimed to determine the relative efficacy of combination regimens and identify an optimal regimen for preventing PEP.MethodsWe performed a systematic and comprehensive search to identify and analyze all the randomized controlled studies published until October 15, 2019, examining rectal NSAIDs and their combination with other pharmacological agents for the prevention of PEP. The primary outcome was the frequency of PEP. We conducted an NMA to combine the direct and indirect comparisons of rectal NSAIDs and their combination with other pharmacological agents.ResultsThe NMA included 24 studies evaluating 14 regimens in 11 321 patients. According to predictive interval plot and surface under the cumulative ranking curve values, indomethacin + lactated Ringer's solution, followed by diclofenac + nitrate and indomethacin + normal saline, is the most efficacious combination of pharmacological agents for the overall prevention of PEP. Rectal indomethacin alone is the most efficacious agent for prevention of moderate to severe PEP, and rectal diclofenac is the most useful agent for prevention of PEP among the high‐risk group.ConclusionsRectal indomethacin with intravenous hydration and rectal diclofenac with sublingual nitrate are the most efficacious combination regimens for the overall prevention of PEP.
An investigation of recommended serve food portions and attaining energy and protein requirements in older adults living in residential care
Authors: Sossen L. , Bonham M. , Porter J. 
Published: October 2020

AbstractBackgroundAgeing populations show a propensity for reduced food intake, which impacts nutritional adequacy. Nutrition guidelines for residential care homes (RCHs) are currently based on serve size of core food groups and do not consider nutrient density. The present study aimed to investigate the weight of foods served/consumed compared to recommended serve sizes and to compare energy and protein intake with individual requirements.MethodsThis was an observational study of older adults living in four RCHs. Dietary intake was estimated through the difference between weighed reference meals and a single, double‐weighed 24‐h food plate waste collected from each participant. FoodWorks9® (Xyris® Software, Brisbane, Australia) was used to calculate energy, protein and serves of core food groups from food intake and the menu provided to recommended serve sizes. Individual intake was compared with nutrition guidelines and estimated energy and protein requirements.ResultsAcross 420 participants, 9.8% completed a main meal (lunch or dinner). The servings provided [248 g; interquartile range (IQR) = 206–290 g] were less than the recommended servings for a main meal (306 g = protein/starch/two vegetables), with 157 g (IQR = 109–221 g) consumed. The menu provided for minimum serves of all core food groups except for dairy. Median energy intake (n = 389) (5272 kJ day−1, IQR = 4229–6720 kJ) and protein intake (47.3 g day−1, IQR = 35.9–60.8 g) were less than estimated requirements (8181 kJ day−1, IQR = 7300–9338 kJ day−1; 76.7 g day−1, IQR = 66.7–90.8 g).ConclusionsNutritional needs were not met in this cohort. The findings of the present study highlight the need for smaller, nutrient‐dense meals and revised menu standards to ensure nutritional adequacy in this vulnerable population.
Characteristics of aquaporin 1, 3, and 5 expression during early murine salivary gland development
Authors: de Paula Fernanda , Tucker Abigail S. , Teshima Tathyane Harumi N. , de Souza Milena Monteiro , Coutinho‐Camillo Cláudia Malheiros , Nico Marcello Menta S. , Lourenço Silvia Vanessa 
Published: October 2020

AbstractAquaporins (AQPs) are essential to coordinate the transit of water and ions through the cell membrane. In salivary glands (SGs), AQPs have been associated with saliva formation, facilitating water absorption through the epithelium during the formation of hypotonic saliva, which is then secreted into the oral cavity. Different members of the AQP family have been suggested to play distinct roles during embryonic development, highlighted by their specific expression patterns. Here, we have investigated the expression patterns of AQP‐1, AQP‐3 and AQP‐5 by immunofluorescence at key stages of salivary gland development, utilising cultured mouse embryonic submandibular (SMG) and sublingual (SLG) glands. The expression of AQPs was compared to a mitotic marker, phospho‐histone 3 (PH3), a myoepithelial marker, smooth muscle actin (SMA), and a vascular marker, CD31. Qualitative analysis revealed that AQP‐1 and AQP‐3 were primarily expressed during the earlier phases of SG morphogenesis and were associated with cells undergoing mitotic processes (PH3‐positive). AQP‐5, in contrast, was not associated to mitotic figures, but was predominantly expressed during late stages of SG morphogenesis. Our results highlight that AQPs are expressed from early stages of SG morphogenesis and exhibit complimentary expression patterns that may contribute to the morphogenesis of salivary glands.
Factors Affecting Adoption of Coordinated Anxiety Learning and Management (CALM) in Veterans’ Affairs Community‐Based Outpatient Clinics
Authors: Ecker Anthony H. , Abraham Traci H. , Martin Lindsey A. , Marchant‐Miros Kathy , Cucciare Michael A. 
Published: October 2020

AbstractPurposeMany US military veterans experience anxiety, depression, and trauma‐related disorders. A major goal of the Veterans Health Administration (VHA) has been to increase access to evidence‐based psychotherapies (EBPs) such as cognitive‐behavioral therapy to address veterans’ substantial health burden. However, despite widespread implementation of EBPs throughout the VHA, smaller clinics that often serve rural veterans face barriers to delivering these interventions. The Veterans Affairs Coordinated Anxiety Learning and Management (VA CALM) program aims to empower providers in rural areas with varying levels of training and experience in delivering EBPs to provide high‐quality cognitive‐behavioral therapy for anxiety, depression, and trauma‐related disorders. The goal of this study was to better understand, through qualitative interviews, VHA community‐based outpatient clinic providers’ perspectives on implementing VA CALM.MethodsQualitative interviews with providers (N = 22) were conducted to understand implementation of VA CALM. Template analysis was used to organize and summarize responses.FindingsProviders noted several facilitators for implementing VA CALM in rural community clinics, including its perceived effectiveness, broad applicability, and structure. Barriers to implementation included scheduling problems and patient‐related barriers.ConclusionsIncorporating providers’ perspectives on factors that affect implementing cognitive‐behavioral therapy in this setting may inform future efforts to disseminate‐implement EBPs in smaller, more remote VHA clinics.
Seasonality, local resources and environmental factors influence patterns of brown bear damages: implications for management
Authors: Zarzo‐Arias A. , Delgado M. M. , Palazón S. , Afonso Jordana I. , Bombieri G. , González‐Bernardo E. , Ordiz A. , Bettega C. , García‐González R. , Penteriani V. 
Published: October 2020

AbstractCoexistence of humans and large carnivores is a major challenge for conservation and management, especially in human‐modified landscapes. Ongoing recovery of some large carnivore populations is good conservation news, but it also brings about increased levels of conflict with humans. Compensation payments and preventive measures are used worldwide as part of conservation programmes with the aim of reducing such conflicts and improving public attitude towards large carnivores. However, understanding the drivers triggering conflicts is a conservation priority, which helps prevent and reduce damages. Here, we have analysed the spatio‐temporal patterns of brown bear Ursus arctos damages to apiaries, crops and livestock in the two small, isolated and endangered bear populations in northern Spain. The increase in the number of damages varied in parallel with the increase in bear numbers, which is probably a primary cause determining the occurrence on damages. Damages also varied among years, seasons and bear populations and seemed to mainly depend on the local availability of natural food items, weather conditions and the availability of apiaries and livestock. Fluctuating availability of food items may explain the frequency of conflicts, which is yet another call to apply preventive measures in carnivore damage to human property in seasons and years when natural food availability is lower than usual. Understanding and preventing damage is in turn essential to mitigate conflicts where humans and large carnivores share the same landscape.
Linking epigenetic signature and metabolic phenotype in IDH mutant and IDH wildtype diffuse glioma
Authors: Braun Yannick , Filipski Katharina , Bernatz Simon , Baumgarten Peter , Roller Bastian , Zinke Jenny , Zeiner Pia S. , Ilina Elena , Senft Christian , Ronellenfitsch Michael W. , Plate Karl H. , Bähr Oliver , Hattingen Elke , Steinbach Joachim P. , Mittelbronn Michel , Harter Patrick N. 
Published: October 2020

AbstractAimsChanges in metabolism are known to contribute to tumour phenotypes. If and how metabolic alterations in brain tumours contribute to patient outcome is still poorly understood. Epigenetics impact metabolism and mitochondrial function. The aim of this study is a characterisation of metabolic features in molecular subgroups of isocitrate dehydrogenase mutant (IDHmut) and isocitrate dehydrogenase wildtype (IDHwt) gliomas.MethodsWe employed DNA methylation pattern analyses with a special focus on metabolic genes, large‐scale metabolism panel immunohistochemistry (IHC), qPCR‐based determination of mitochondrial DNA copy number and immune cell content using IHC and deconvolution of DNA methylation data. We analysed molecularly characterised gliomas (n = 57) for in depth DNA methylation, a cohort of primary and recurrent gliomas (n = 22) for mitochondrial copy number and validated these results in a large glioma cohort (n = 293). Finally, we investigated the potential of metabolic markers in Bevacizumab (Bev)‐treated gliomas (n = 29).ResultsDNA methylation patterns of metabolic genes successfully distinguished the molecular subtypes of IDHmut and IDHwt gliomas. Promoter methylation of lactate dehydrogenase A negatively correlated with protein expression and was associated with IDHmut gliomas. Mitochondrial DNA copy number was increased in IDHmut tumours and did not change in recurrent tumours. Hierarchical clustering based on metabolism panel IHC revealed distinct subclasses of IDHmut and IDHwt gliomas with an impact on patient outcome. Further quantification of these markers allowed for the prediction of survival under anti‐angiogenic therapy.ConclusionA mitochondrial signature was associated with increased survival in all analyses, which could indicate tumour subgroups with specific metabolic vulnerabilities.
Regulation of shoot branching in arabidopsis by trehalose 6‐phosphate
Authors: Fichtner Franziska , Barbier Francois F. , Annunziata Maria G. , Feil Regina , Olas Justyna J. , Mueller‐Roeber Bernd , Stitt Mark , Beveridge Christine A. , Lunn John E. 
Published: October 2020

Summary

Trehalose 6‐phosphate (Tre6P) is a sucrose signalling metabolite that has been implicated in regulation of shoot branching, but its precise role is not understood.

We expressed tagged forms of TREHALOSE‐6‐PHOSPHATE SYNTHASE1 (TPS1) to determine where Tre6P is synthesized in arabidopsis (Arabidopsis thaliana), and investigated the impact of localized changes in Tre6P levels, in axillary buds or vascular tissues, on shoot branching in wild‐type and branching mutant backgrounds.

TPS1 is expressed in axillary buds and the subtending vasculature, as well as in the leaf and stem vasculature. Expression of a heterologous Tre6P phosphatase (TPP) to lower Tre6P in axillary buds strongly delayed bud outgrowth in long days and inhibited branching in short days. TPP expression in the vasculature also delayed lateral bud outgrowth and decreased branching. Increased Tre6P in the vasculature enhanced branching and was accompanied by higher expression of FLOWERING LOCUS T (FT) and upregulation of sucrose transporters. Increased vascular Tre6P levels enhanced branching in branched1 but not in ft mutant backgrounds.

These results provide direct genetic evidence of a local role for Tre6P in regulation of axillary bud outgrowth within the buds themselves, and also connect Tre6P with systemic regulation of shoot branching via FT.
Attitudes toward anticoagulation for postoperative atrial fibrillation: A nationwide survey of VA providers
Authors: Riad Fady S. , German Konstantin , Deitz Sarah , Sahadevan Jayakumar , Sundaram Varun , Waldo Albert L. 
Published: October 2020

AbstractIntroductionPostoperative atrial fibrillation (POAF) is a common complication after cardiac surgery. Though often felt to be self‐limited, this complication has been associated with increases in both short and long‐term stroke and mortality. Several studies have also shown a high rate of AF recurrence. Optimal treatment strategy is not yet defined, and the role of anticoagulation (AC) is unclear. Our objective was to determine provider attitudes toward management of this common complication.MethodsA survey consisting of 15 multiple choice questions was distributed to providers at Veterans Healthcare Administration hospitals nationwide.ResultsThe majority of respondents were cardiologists. Practices varied drastically with respect to AC use for patients with POAF who were discharged in normal sinus rhythm. Less variability existed for patients discharged in AF. There was no clear consensus regarding other factors to consider when deciding on AC therapy, including length of episode, or risk factors for stroke such as CHA2DS2‐VASc score. There was also no consensus on duration of therapy or need for post discharge cardiac monitoring.ConclusionOur data indicate a wide variability in the management of POAF. This reflects conflicting recommendations in the guidelines, as well as a paucity of prospective treatment trials in this field. Nevertheless, a growing evidence base suggests that this complication carries potentially serious long‐term morbidity and mortality, and better evidence for its management is needed.
Multivariate models for estimating jackass semen production and quality
Authors: Liu Qing , Yu Jie , Li Min , Zhang Ruitao , Gao Peng , Cong Guanglei , Cui Daiyong , Ji Chuanliang , Zeng Shenming 
Published: October 2020

AbstractThe purpose of this study was to analyse the effects of season, age, gonad and accessory sex glands on semen characteristics of jackass and to construct multivariate regression models to predict semen quality. In autumn, spring and summer, semen characteristics of 30 sexually mature donkeys (1,014 ejaculations) were analysed to investigate the effect of seasons on semen quality, and gonad and accessory sex gland parameters of 12 jackasses were measured immediately after ejaculation by ultrasonography to investigate the effect of seasons on reproductive organ size. Semen (598 ejaculates), gonad and accessory sex gland parameters of 40 jackasses aged between 3 and 7 years were analysed in autumn to investigate the effects of age and reproductive organ size on semen quality and to construct multivariate models. To verify the accuracy of the models, semen (476 ejaculates), gonad and accessory sex gland parameters of 20 jackasses were measured from March to June. Results revealed that semen, gonad and accessory sex gland parameters were not affected by season and age. Progressive motility (PM) was positively correlated with long axis of the spermatic cord (LASC) and negatively correlated with percentages of sperm abnormality (PSA). Total sperm count (TSC) was positively correlated with testis circumferences (TC) and cross‐sectional area of cauda epididymis (CSACE). TC, CSACE, LASC and PSA were included into multivariate models to predict PM, TSC and functional sperm count (FSC) in 20 jackasses (PM = 72.332 + 0.428 LASC − 0.441 PSA; TSC = −169.929 + 8.728 TC + 0.253 CSACE; FSC = −206.645 + 8.788 TC + 0.258 CSACE). The predicted and observed values corresponded well. In conclusion, the tested models can be used for predicting semen quality of donkey.