bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi‐omics analysis suggests enhanced epileptogenesis in the Cornu Ammonis 3 of the pilocarpine model of mesial temporal lobe epilepsy
Authors: Canto Amanda M. , Matos Alexandre H. B. , Godoi Alexandre B. , Vieira André S. , Aoyama Beatriz B. , Rocha Cristiane S. , Henning Barbara , Carvalho Benilton S. , Pascoal Vinicius D.B. , Veiga Diogo F. T. , Gilioli Rovilson , Cendes Fernando , Lopes‐Cendes Iscia 
Published: October 2020

AbstractMesial temporal lobe epilepsy (MTLE) is a chronic neurological disorder characterized by the occurrence of seizures, and histopathological abnormalities in the mesial temporal lobe structures, mainly hippocampal sclerosis (HS). We used a multi‐omics approach to determine the profile of transcript and protein expression in the dorsal and ventral hippocampal dentate gyrus (DG) and Cornu Ammonis 3 (CA3) in an animal model of MTLE induced by pilocarpine. We performed label‐free proteomics and RNAseq from laser‐microdissected tissue isolated from pilocarpine‐induced Wistar rats. We divided the DG and CA3 into dorsal and ventral areas and analyzed them separately. We performed a data integration analysis and evaluated enriched signaling pathways, as well as the integrated networks generated based on the gene ontology processes. Our results indicate differences in the transcriptomic and proteomic profiles among the DG and the CA3 subfields of the hippocampus. Moreover, our data suggest that epileptogenesis is enhanced in the CA3 region when compared to the DG, with most abnormalities in transcript and protein levels occurring in the CA3. Furthermore, our results show that the epileptogenesis in the pilocarpine model involves predominantly abnormal regulation of excitatory neuronal mechanisms mediated by N‐methyl D‐aspartate (NMDA) receptors, changes in the serotonin signaling, and neuronal activity controlled by calcium/calmodulin‐dependent protein kinase (CaMK) regulation and leucine‐rich repeat kinase 2 (LRRK2)/WNT signaling pathways.
Do myeloproliferative neoplasms and multiple myeloma share the same genetic susceptibility loci?
Authors: Macauda Angelica , Giaccherini Matteo , Sainz Juan , Gemignani Federica , Sgherza Nicola , Sánchez‐Maldonado José Manuel , Gora‐Tybor Joanna , Martinez‐Lopez Joaquin , Carreño‐Tarragona Gonzalo , Jerez Andrés , Spadano Raffaele , Gołos Aleksandra , Jurado Manuel , Hernández‐Mohedo Francisca , Mazur Grzegorz , Tavano Francesca , Butrym Aleksandra , Várkonyi Judit , Canzian Federico , Campa Daniele 
Published: October 2020

AbstractMyeloproliferative neoplasms (MPNs) are a group of diseases that cause myeloid hematopoietic cells to overproliferate. Epidemiological and familial studies suggest that genetic factors contribute to the risk of developing MPN, but the genetic susceptibility of MPN is still not well known. Indeed, only few loci are known to have a clear role in the predisposition to this disease. Some studies reported a diagnosis of MPNs and multiple myeloma (MM) in the same patients, but the biological causes are still unclear. We tested the hypothesis that the two diseases share at least partly the same genetic risk loci. In the context of a European multicenter study with 460 cases and 880 controls, we analyzed the effect of the known MM risk loci, individually and in a polygenic risk score (PRS). The most significant result was obtained among patients with chronic myeloid leukemia (CML) for PS0RS1C1‐rs2285803, which showed to be associated with an increased risk (OR = 3.28, 95% CI 1.79‐6.02, P = .00012, P = .00276 when taking into account multiple testing). Additionally, the PRS showed an association with MPN risk when comparing the last with the first quartile of the PRS (OR = 2.39, 95% CI 1.64‐3.48, P = 5.98 × 10−6). In conclusion, our results suggest a potential common genetic background between MPN and MM, which needs to be further investigated.
Weight change in middle adulthood and risk of cancer in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort
Authors: Christakoudi Sofia , Pagoni Panagiota , Ferrari Pietro , Cross Amanda J. , Tzoulaki Ioanna , Muller David C. , Weiderpass Elisabete , Freisling Heinz , Murphy Neil , Dossus Laure , Turzanski Fortner Renee , Agudo Antonio , Overvad Kim , Perez‐Cornago Aurora , Key Timothy J. , Brennan Paul , Johansson Mattias , Tjønneland Anne , Halkjær Jytte , Boutron‐Ruault Marie‐Christine , Artaud Fanny , Severi Gianluca , Kaaks Rudolf , Schulze Matthias B. , Bergmann Manuela M. , Masala Giovanna , Grioni Sara , Simeon Vittorio , Tumino Rosario , Sacerdote Carlotta , Skeie Guri , Rylander Charlotta , Borch Kristin Benjaminsen , Quirós J. Ramón , Rodriguez‐Barranco Miguel , Chirlaque Maria‐Dolores , Ardanaz Eva , Amiano Pilar , Drake Isabel , Stocks Tanja , Häggström Christel , Harlid Sophia , Ellingjord‐Dale Merete , Riboli Elio , Tsilidis Konstantinos K. 
Published: October 2020

AbstractObesity is a risk factor for several major cancers. Associations of weight change in middle adulthood with cancer risk, however, are less clear. We examined the association of change in weight and body mass index (BMI) category during middle adulthood with 42 cancers, using multivariable Cox proportional hazards models in the European Prospective Investigation into Cancer and Nutrition cohort. Of 241 323 participants (31% men), 20% lost and 32% gained weight (>0.4 to 5.0 kg/year) during 6.9 years (average). During 8.0 years of follow‐up after the second weight assessment, 20 960 incident cancers were ascertained. Independent of baseline BMI, weight gain (per one kg/year increment) was positively associated with cancer of the corpus uteri (hazard ratio [HR] = 1.14; 95% confidence interval: 1.05‐1.23). Compared to stable weight (±0.4 kg/year), weight gain (>0.4 to 5.0 kg/year) was positively associated with cancers of the gallbladder and bile ducts (HR = 1.41; 1.01‐1.96), postmenopausal breast (HR = 1.08; 1.00‐1.16) and thyroid (HR = 1.40; 1.04‐1.90). Compared to maintaining normal weight, maintaining overweight or obese BMI (World Health Organisation categories) was positively associated with most obesity‐related cancers. Compared to maintaining the baseline BMI category, weight gain to a higher BMI category was positively associated with cancers of the postmenopausal breast (HR = 1.19; 1.06‐1.33), ovary (HR = 1.40; 1.04‐1.91), corpus uteri (HR = 1.42; 1.06‐1.91), kidney (HR = 1.80; 1.20‐2.68) and pancreas in men (HR = 1.81; 1.11‐2.95). Losing weight to a lower BMI category, however, was inversely associated with cancers of the corpus uteri (HR = 0.40; 0.23‐0.69) and colon (HR = 0.69; 0.52‐0.92). Our findings support avoiding weight gain and encouraging weight loss in middle adulthood.
Debt stabilization and financial stability in a monetary union: Market versus authority‐based preventive solutions
Authors: Mavrodimitrakis Christos 
Published: October 2020

AbstractIs a debt‐concerned monetary authority desirable? This article deals with the impact of fiscal‐monetary policy interactions in a monetary union on country‐specific and union‐wide debt stabilization, when the authorities act strategically. The focus is on the impact that monetary policy would have on a debt‐concerned fiscal authority through the debt constraint and the corresponding interaction with decentralized fiscal policies. It is shown that the fiscal‐monetary (overall) policy coordination strategic regime delivers better results than the non‐cooperative regime for both the output gap and the outstanding level of debt at the union level. Two further institutional arrangements are investigated and compared: An authority‐based preventive procedure, which works through a debt‐concerned monetary authority, and a market‐based one, which works through financial markets by assuming a risk premium on country‐specific nominal interest rates according to the country‐specific fiscal stances. The risk premium acts as a form of ‘market‐based’ discipline. Both regimes stand between the decentralized and the centralized setting. The central bank's optimal weight on union‐wide debt stabilization is computed when financial markets provide discipline to the fiscal authorities. It is optimal for the social planner to appoint a debt‐concerned central banker, if there is a degree of conservatism from the monetary authority, and/or if the social planner cares about union‐wide debt stabilization. A higher risk premium parameter enhances the impact of the former on the optimal weight on union‐wide debt stabilization, while it reduces the impact of the latter.
Evaluating stock trading behaviour: Information sources nexus through intrinsic and extrinsic motivation
Authors: Khan Safeer Ullah , Wang Mansi , Khan Ikram Ullah , Liu Xiang‐dong 
Published: October 2020

AbstractThis study examines the effects of sources of information, including word‐of‐mouth (WOM) communication, and financial advice, on intrinsic and extrinsic motivations and stock trading behaviour in Pakistan. To test the model, we gathered 477 survey responses from individual stock investors. Our results exhibit that WOM communication and financial advice significantly influence stock trading behaviour. We also find that intrinsic motivations significantly mediate between financial advice and stock trading behaviour. Extrinsic motivations have significant mediation effects between sources of information and stock trading behaviour. Taken together, the results indicate that financial advice indirectly sparks stock trading through intrinsic and extrinsic motivations, whereas WOM communication performs the same function only through extrinsic motivations. The study results have several theoretical and practical implications for investors, stock brokerage firms, regulators, and governments.
2D+t track detection via relative persistent homology
Authors: Assaf Rabih , Goupil Alban , Rammal Abbas , Vrabie Valeriu , Kacim Mohammad 
Published: October 2020

AbstractIn this paper, we demonstrate that algebraic topology can be used to perform 2D+t object detection. After the construction of a topological complex for a 2D+t image sequence, we build a nested sequence of cell complexes on which relative persistent homology is computed. The relative homology adds to “absolute” homology the computation of classes related to the first and last frames of the sequence. By identifying 2D chains with large life spans, the most persistent classes are extracted. This allows for the identification of the interesting parts in a sequence and for the detection of the movement of objects despite continuous deformations in the image domain. The results obtained on a synthetic image and on two real biomedical images with moving vesicles recorded by a quantitative phase time‐lapse technique show the potential of this method. Comparing the method with a newly developed tracking tool proves that the strength of this method is its independence from prior parameters.
3D immuno‐confocal image reconstruction of fibroblast cytoskeleton and nucleus architecture
Authors: Markov Petar , Hayes Anthony J. , Zhu Hanxing , Boote Craig , Blain Emma J. 
Published: October 2020

AbstractComputational models of cellular structures generally rely on simplifying approximations and assumptions that limit biological accuracy. This study presents a comprehensive image processing pipeline for creating unified three‐dimensional (3D) reconstructions of the cell cytoskeletal networks and nuclei. Confocal image stacks of these cellular structures were reconstructed to 3D isosurfaces (Imaris), then tessellations were simplified to reduce the number of elements in initial meshes by applying quadric edge collapse decimation with preserved topology boundaries (MeshLab). Geometries were remeshed to ensure uniformity (Instant Meshes) and the resulting 3D meshes exported (ABAQUS) for downstream application. The protocol has been applied successfully to fibroblast cytoskeletal reorganisation in the scleral connective tissue of the eye, under mechanical load that mimics internal eye pressure. While the method herein is specifically employed to reconstruct immunofluorescent confocal imaging data, it is also more widely applicable to other biological imaging modalities where accurate 3D cell structures are required.
The role of negative cognitions in co‐occurring posttraumatic stress disorder and depression: Examination of interpersonal and noninterpersonal trauma survivors
Authors: Majeed Rimsha , Lipinski Alexandra J. , Free Bre'Anna L. , Lewin Rivian K. , Beck J. Gayle 
Published: October 2020

AbstractObjectiveTo examine negative cognitions underlying both posttraumatic stress disorder (PTSD) and depression following trauma.MethodA mixed‐gender motor vehicle accident (N = 297, Mage = 43.49 years) sample and a female intimate partner violence (N = 242, Mage = 36.95 years) sample was cross‐sectionally studied at research clinics of two universities.ResultsWhen diagnostic groups (PTSD+/−, depression+/−) were studied, no significant interactions were noted for any of the three forms of negative cognitions (negative thoughts about the self, negative thoughts about the world, and self‐blame) in either sample. When continuous ratings of PTSD and depression were studied, the results showed that negative thoughts about the self were linked to both PTSD and depression.ConclusionFindings suggest that it may be beneficial to target negative thoughts about the self when treating PTSD and depression together.
Effects of exercise on exosome release and cargo in in vivo and ex vivo models: A systematic review
Authors: Estébanez Brisamar , Jiménez‐Pavón David , Huang Chun‐Jung , Cuevas María J. , González‐Gallego Javier 
Published: October 2020

AbstractExercise‐released exosomes have been identified as novel players to mediate cell‐to‐cell communication in promoting systemic beneficial effects. This review aimed to systematically investigate the effects of exercise on exosome release and cargo, as well as provide an overview of their physiological implications. Among the 436 articles obtained in the database search (WOS, Scopus, and PubMed), 19 articles were included based on eligibility criteria. Results indicate that exercise promotes the release of exosomes without modification of its vesicle size. The literature has primarily shown an exercise‐driven increase in exosome markers (Alix, CD63, CD81, and Flot‐1), along with other exosome‐carried proteins, into circulation. However, exosome isolation, characterization, and phenotyping methodology, as well as timing of sample recovery following exercise can influence the analysis and interpretation of findings. Moreover, a large number of exosome‐carried microRNAs (miRNAs), including miR‐1, miR‐133a, miR‐133b, miR‐206, and miR‐486, in response to exercise are involved in the modulation of proliferation and differentiation of skeletal muscle tissue, although antigen‐presenting cells, leukocytes, endothelial cells, and platelets are the main sources of exosome release into the circulation. Collectively, with the physiological implications as evidenced by the ex vivo trials, the release of exercise‐promoted exosomes and their cargo could provide the potential therapeutic applications via the role of intercellular communication.
Novel approach for neuronal stem cell differentiation using aqueous two‐phase systems in 3D cultures
Authors: Chairez‐Cantu Karolina , González‐González Mirna , Rito‐Palomares Marco 
Published: October 2020

AbstractStem cell therapy has emerged as a promising alternative for replacing lost cells involved in neurodegenerative diseases. High efficiency of differentiation and full cell viability are actual challenges to achieve the translation of cell therapies to the clinic. To address this, the construction of aqueous two‐phase systems in three‐dimensional (ATPS‐3D) cultures has been proposed. This technique involves the combination of two polymers in which cells are confined in dextran droplets immersed over a substrate located in a poly(ethylene glycol) phase. The controlled placement of cells in a defined pattern promotes intercellular communication. This review aims to provide insight into the techniques used to enhance neural differentiation and current challenges to achieve the implementation of cell therapies. Cell density, colony size, interconnectivity and an appropriate substrate to modulate paracrine signaling are factors that determine neural differentiation efficiency during the construction of ATPS‐3D cultures. Hence, this contact‐free technique enables the design of neural niches to recapitulate in vivo environments more accurately. © 2020 Society of Chemical Industry (SCI)
Prenatal diagnosis of aortic arch anomalies: Echocardiography, 3D‐ultrasonography, and computed tomography angiogram findings. A case‐report
Authors: Bravo‐Valenzuela Nathalie Jeanne , Nacif Marcelo Souto , Araujo Júnior Edward 
Published: October 2020

AbstractWe report the case of a fetus presenting aortic arch anomalies associated with a ventricular septal defect (VSD). This fetus, which was referred at 25 weeks of gestation, was suspected to have coarctation of aorta (CoA) evidenced by enlarged right chambers at the four‐chamber view during a routine obstetric ultrasonographic scan. The prenatal diagnosis of CoA remains a challenge. Here, we review the ultrasonographic findings that could contribute to this diagnosis.
Long non‐coding RNA MCF2L‐AS1 promotes the aggressiveness of colorectal cancer by sponging miR‐874‐3p and thereby up‐regulating CCNE1
Authors: Huang Fa‐Kun , Zheng Cheng‐Ying , Huang Long‐Kai , Lin Chang‐Qing , Zhou Jun‐Feng , Wang Jia‐Xing 
Published: October 2020

AbstractBackgroundLong non‐coding RNAs (lncRNAs) have drawn growing attention because of the role which they play in various diseases, including colorectal cancer (CRC). However, the potential functions of lncRNA MCF2L antisense RNA 1 (MCF2L‐AS1) in tumors remained largely unclear. The present study aimed to explore the clinical significance and the biological effects of lncRNA MCF2L antisense RNA 1 (MCF2L‐AS1) in CRC.MethodsReverse transcriptase‐polymerase chain reaction was performed to determine the expression of MCF2L‐AS1 in CRC. The clinical significance of MCF2L‐AS1 in CRC patients was analyzed statistically. In vitro experiments were performed to determine the effects of MCF2L‐AS1 on the cellular progression of CRC cells. Bioinformatic assays, luciferase reporter assays and RNA‐pulldown assays were performed to predict for potential microRNAs that can interact with MCF2L‐AS1 and mRNAs that can interact with miR‐874‐3p.ResultsWe identified a novel CRC‐related lncRNA, MCF2L‐AS1, which is distinctly highly expressed in CRC. Its diagnostic value for CRC patients was also demonstrated. Clinical assays revealed that high MCF2L‐AS1 expression is associated with advanced stages, positive metastasis and the poor prognosis of CRC patients. Multivariate assays confirmed that MCF2L‐AS1 expression is an independent poor prognostic factor for both 5‐year overall survival and 5‐year disease‐free survival of CRC patients. Functionally, we confirmed that knockdown of MCF2L‐AS1 distinctly suppresses the proliferation, migration and invasion of CRC cells and also promotes apoptosis. Mechanistic investigation showed that MCF2L‐AS1 functions as an endogenous sponge for miR‐874‐3p to increase the expression of CCNE1.ConclusionsOur findings identified a novel CRC‐related lncRNA, MCF2L‐AS1, which may be used as a potential diagnostic and prognostic biomarker for CRC patients. In addition, the newly identified MCF2L‐AS1/miR‐874‐3p/CCNE1 axis can modulate the initiation and progression of CRC.
Profiling of specific long non‐coding RNA signatures identifies ST8SIA6‐AS1 AS a novel target for breast cancer
Authors: Chen Zhanghan , Huang Jie , Feng Yanling , Li Zehuan , Jiang Ying 
Published: October 2020

AbstractBackgroundBreast cancer is the most commonly diagnosed cancer among women and is also the leading cause of cancer death for which the treatment and methods of diagnosis remain unsatisfied. Long non‐coding RNA (lncRNA) plays an important role in the occurrence and development of tumors, including breast cancer. We aimed to seek new and efficient treatment targets by analyzing the lncRNA expression profiles of breast cancer.MethodsA competitive endogenous RNA microarray was used to investigate the profiles of differentially expressed lncRNAs. Quantitative real‐time polymerase chain reaction analysis (qRT‐PCR) validated the top differentially expressed lncRNAs in 107 pairs of breast cancer tissues and adjacent normal tissues. cis‐ and trans‐regulation mRNAs of lncRNAs were used to perform enrichment analysis. Cell function assays were used to explore the functions of ST8SIA6‐AS1.ResultsSeven lncRNAs, comprising ST8SIA6‐AS1, lnc‐HIST1H2BJ‐5:1, lnc‐PRICKLE2‐3:2, RP1‐86C11.7, RP11‐15F12.1, ZNF670‐ZNF695 and lnc‐STRN3‐12:1, were shown to be significantly up‐regulated in breast cancer. lncRNA ST8SIA6‐AS1 was associated with TNM staging and Ki‐67 index. The cell function assays showed that ST8SIA6‐AS1 can promote the proliferation, migration and invasion of breast cancer cells. The functions of ST8SIA6‐AS1 were explored and the competing endogenous RNA mode showed that miR‐4252 was a potential candidate. Its target genes were further predicted. The lncRNA‐protein mode showed three potential candidate RNA binding proteins: NONO, QKI and RBMX.ConclusionslncRNA ST8SIA6‐AS1 can promote the proliferation, migration and invasion of breast cancer cells. By hypothesizing two different functional modes of ST8SIA6‐AS1, we found lncRNA ST8SIA6‐AS1 may contribute to breast cancer progression through miR‐4252 or interacting with RNA binding proteins: NONO, QKI and RBMX.
Therapeutic Encouragement as Conceptualized by South Korean Youth Counselors
Authors: Jeung Shin Young , Lee Dong‐gwi , Ham Kyong‐ae , Kim Jin Hwa 
Published: October 2020

This study explored counselors’ conceptualization and use of encouragement as a therapeutic tool. Responses to two questions from 100 South Korean youth counselors were analyzed using consensual qualitative research–modified. As a result, six categories emerged under each domain. Implications for counseling and future studies are discussed.Este estudio exploró la conceptualización y el uso del apoyo como herramienta terapéutica por parte los consejeros. Usando el método modificado de investigación cualitativa consensual, se analizaron las respuestas proporcionadas a dos preguntas por 100 consejeros surcoreanos que trabajan con jóvenes. Como resultado, surgieron seis categorías bajo cada dominio. Se discuten las implicaciones para la consejería e investigaciones futuras.
The Integrated Acculturation Model: Expanding Acculturation to Cultural Identities in Addition to Race and Ethnicity
Authors: Garcia Jorge G. , DiNardo Jeff , Nuñez Maria Inmaculada Lopez , Emmanuel Diona , Chan Christian D. 
Published: October 2020

This article is an attempt to integrate existing conceptual knowledge on acculturation as well as to expand the use of the construct, which has traditionally had race and ethnicity as its focus, to also include cultural identities such as sexual orientation, gender identity, nationality, and disability. The article begins with a review of existing theoretical contributions from both the United States and Europe. We then propose a model to update and expand the use of acculturation. Finally, we provide an assessment framework.Este artículo es un intento de integrar los conocimientos conceptuales existentes sobre la aculturación además de expandir el uso de este constructo, que tradicionalmente se ha enfocado hacia la raza y la etnicidad, para incluir también identidades culturales tales como la orientación sexual, la identidad de sexo, la nacionalidad y la discapacidad. El artículo comienza con una revisión de las contribuciones teóricas existentes tanto de Estados Unidos como de Europa. A continuación, proponemos un modelo para actualizar y expandir el uso de la aculturación. Por último, proporcionamos un marco de evaluación.