bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of KLF14 in multiple disease processes
Authors: Chen Xiaoyan , Shi Wenjie , Zhang Heng 
Published: January 2020

AbstractKruppel‐like factor 14 (KLF14) is a newly identified member of the KLF family. Expression of KLF14 is induced by TGF‐β in intrauterine and ectodermal tissue. Initial researches on KLF14 focused on its role in lipid and glucose metabolism. In recent years, however, the role of KLF14 in regulating cell signaling pathways, cell proliferation and differentiation has been explored. Moreover, the research has gradually extended into the field of tumorigenesis and immune regulation. This paper aims to briefly review the functions of KLF14 in physiologyical and pathological process.
Optimized immobilization of endoglucanase Cel9A onto glutaraldehyde activated chitosan nanoparticles by response surface methodology: The study of kinetic behaviors
Authors: Najavand Saeed , Habibnejad Mahdiyeh , Amani‐Ghadim A. R. , Rahimizadeh Parastou , Pazhang Mohammad 
Published: January 2020

AbstractImmobilization of enzyme onto nanoparticles such as chitosan can have biotechnological importance. In this study, chitosan nanoparticles (ChNPs) were prepared by Ionic gelation method and Endoglucanase Cel9A from Alicyclobacillus acidocaldariius (AaCel9A) immobilized on the nanoparticles. The FTIR results showed that the enzymes were immobilized on the ChNPs. The dynamic light scattering and scanning electron microscope (SEM) results illustrated that the AaCel9A‐ChNPs approximately had 40 nm diameters. For optimizing enzyme immobilization, response surface methodology was employed using different variables (pH, enzyme immobilization time, and enzyme to ChNPs ratio [E/Cs]). The results showed that the high immobilization efficiency was achieved in pH 7, E/Cs of 0.4 in 2.63 hr. The enzyme activity results showed that, immobilization increased optimum pH for activity (from 6.5 to 7.5) and the enzyme Km (from 3.703 to 12.195 [mg/ml]), which make it suitable to use in some industries such as detergents.
End‐of‐life quality metrics among medicare decedents at minority‐serving cancer centers: A retrospective study
Authors: Wasp Garrett T. , Alam Shama S. , Brooks Gabriel A. , Khayal Inas S. , Kapadia Nirav S. , Carmichael Donald Q. , Austin Andrea M. , Barnato Amber E. 
Published: January 2020

AbstractBackgroundWe calculated the performance of National Cancer Institute (NCI)/National Comprehensive Cancer Network (NCCN) cancer centers’ end‐of‐life (EOL) quality metrics among minority and white decedents to explore center‐attributable sources of EOL disparities.MethodsWe conducted a retrospective cohort study of Medicare beneficiaries with poor‐prognosis cancers who died between April 1, 2016 and December 31, 2016 and had any inpatient services in the last 6 months of life. We attributed patients’ EOL treatment to the center at which they received the preponderance of EOL inpatient services and calculated eight risk‐adjusted metrics of EOL quality (hospice admission ≤3 days before death; chemotherapy last 14 days of life; ≥2 emergency department (ED) visits; intensive care unit (ICU) admission; or life‐sustaining treatment last 30 days; hospice referral; palliative care; advance care planning last 6 months). We compared performance between patients across and within centers.ResultsAmong 126,434 patients, 10,119 received treatment at one of 54 NCI/NCCN centers. In aggregate, performance was worse among minorities for ED visits (10.3% vs 7.4%, P < .01), ICU admissions (32.9% vs 30.4%, P = .03), no hospice referral (39.5% vs 37.0%, P = .03), and life‐sustaining treatment (19.4% vs 16.2%, P < .01). Despite high within‐center correlation for minority and white metrics (0.61‐0.79; P < .01), five metrics demonstrated worse performance as the concentration of minorities increased: ED visits (P = .03), ICU admission (P < .01), no hospice referral (P < .01), and life‐sustaining treatments (P < .01).ConclusionEOL quality metrics vary across NCI/NCCN centers. Within center, care was similar for minority and white patients. Minority‐serving centers had worse performance on many metrics.
In vitro evaluation of antidiabetic potential of hesperidin and its aglycone hesperetin under oxidative stress in skeletal muscle cell line
Authors: Dhanya R. , Jayamurthy P. 
Published: January 2020

The present study investigates the in vitro antidiabetic and antioxidant potential of hesperidin and hesperetin under oxidative stress induced in L6 myotubes. Also, the study attempts to reveal the effect of glycosylation (hesperetin) on the biological activities of hesperidin. Oxidative stress is the leading cause of complications associated with diabetes. Both hesperidin and hesperetin reduce oxidative stress directly by scavenging intracellular reactive oxygen species (ROS) and by up‐regulating natural antioxidant defence system like glutathione. Hesperidin and hesperetin at 10μM inhibited the non‐enzymatic glycation of proteins (65.57% and 35.6%, respectively), the critical reaction involved in the formation of advanced glycation end products (AGEs) which has a significant role in the pathogenesis of diabetes. Additionally, these compounds induced glucose uptake in L6 myotubes following acute and chronic treatment. The percentage 2‐NBDG uptake shown by both the compounds was comparable with that of the antidiabetic drug, rosiglitazone (30.4%). Both the compounds downregulated PI3 kinase activity whereas GLUT4, IRS, and AKT were upregulated in L6 myotubes pointing to the possible overlapping with the insulin signalling pathway.Significance of the studyEvidence suggest that oxidative stress occurs in diabetes and could have a role in the development of insulin resistance. Oral hypoglycaemic agents which target on increasing insulin levels and improving insulin sensitivity or that reduce the rate of carbohydrate absorption from the gastrointestinal tract are used to manage type 2 diabetes. But these therapies rarely target the real cause of type 2 diabetes and have severe adverse effects. The observations from the present study provide significant evidence for hesperidin and hesperetin, to be considered as a dietary supplement to manage type 2 diabetes and to suppress oxidative stress mediated diabetic pathophysiology.
MiR‐643 inhibits lipopolysaccharide‐induced endometritis progression by targeting TRAF6
Authors: Zhao Rui , Wang Jing , Zhang Xiaojuan , Chen Yang 
Published: January 2020

AbstractEndometritis is a prevalent disease with inflammation of uterus endangering women reproductive health. MicroRNAs (miRNAs) play important roles in inflammatory disorders, including endometritis. However, the role and mechanism of miR‐643 in endometritis development remain unclear. This study aimed to investigate the effect of miR‐643 on lipopolysaccharide (LPS)‐induced inflammatory response and clarify the potential mechanism. LPS‐treated human endometrial epithelial cells (HEECs) were cultured to investigate the role of miR‐643 in vitro. The expression levels of miR‐643 and tumor necrosis factor receptor‐associated factor 6 (TRAF6) were measured via quantitative real‐time polymerase chain reaction and western blot, respectively. LPS‐induced inflammatory response was assessed by inflammatory cytokines secretion via enzyme‐linked immunosorbent assay. The activation of nuclear factor‐κB (NF‐κB) pathway was investigated by western blot. The interaction between miR‐643 and TRAF6 was validated by bioinformatics analysis, luciferase reporter assay, and RNA immunoprecipitation. The expression of miR‐643 was decreased and TRAF6 protein level was enhanced in LPS‐treated HEECs. The overexpression of miR‐643 suppressed LPS‐induced secretion of inflammatory cytokines (tumor necrosis factor‐α, interleukin‐1β [IL‐1β], and IL‐6) and activation of NF‐κB pathway. The knockdown of TRAF6 inhibited LPS‐induced inflammatory response in HEECs. TRAF6 was validated as a target of miR‐643 and TRAF6 restoration reversed the effect of miR‐643 on inflammation response in LPS‐treated HEECs. Collectively, miR‐643 attenuated LPS‐induced inflammatory response by targeting TRAF6, indicating a novel avenue for the treatment of endometritis.
Influence of Hydrogen Bond Donating Sites in UiO‐66 Metal‐Organic Framework for Highly Regioselective Methanolysis of Epoxides
Authors: Das Aniruddha , Anbu Nagaraj , Sk Mostakim , Dhakshinamoorthy Amarajothi , Biswas Shyam 
Published: January 2020

AbstractA Zr(IV)‐based UiO‐66 metal‐organic framework (MOF) (named 1) was synthesized by employing 1‐(aminomethyl)naphthalene‐2‐ol appended terephthalate linker and Zr(IV) salt via solvothermal method and subsequently characterized. Furthermore, the potential efficiency of activated (named 1′) form of as‐synthesized MOF was investigated as an organocatalyst for the ring‐opening of epoxide by methanol. The catalytic performance of 1 and 1′ was studied in the methanolysis of styrene oxide as a model substrate and the activity of 1′ was also examined with various alcohols. Under the optimized reaction conditions, the catalytic performance of 1′ reached 96 % conversion of styrene oxide to its corresponding product with 98 % regioselectivity. The reusability and stability of the catalyst were proved by recycling up to four runs in the methanolysis of styrene oxide. The Lewis acidity originating from metal nodes and hydrogen bond donating (HBD) sites in the linker is distributed homogeneously throughout the framework, thus playing crucial role in the activation of epoxide with easy accessibility.
Desulfurization of Model Oil through Adsorption over Activated Charcoal and Bentonite Clay Composites
Authors: Ullah Sana , Hussain Sajjad , Ahmad Waqas , Khan Hammad , Khan Khurram Imran , Khan Saad Ullah , Khan Sabir 
Published: January 2020

AbstractAdsorption of dibenzothiophene (DBT) from model oil was investigated using composites of pure activated charcoal and pure bentonite clay. DBT adsorption was carried out in batch mode experiments at laboratory scale, where the developed composite materials showed a synergistic effect in removal of DBT from the model oil in terms of improved surface acidity of the pure activated charcoal and mesoporous structure of the pure bentonite clay. Thermodynamics, kinetics, and optimization of various adsorption parameters were investigated. Kinetic analyses proved that DBT adsorption followed pseudo‐second‐order kinetics. To study the thermodynamics of the adsorption, different isotherm adsorption models were applied. The Langmuir isotherm best fitted to the adsorption data. Various thermodynamic parameters were evaluated, including Gibbs free energy, entropy, and enthalpy.
Ab Initio Analysis of Metal–Ligand Bonding in An(COT)2 with An=Th, U in Their Ground‐ and Core‐Excited States
Authors: Ganguly Gaurab , Sergentu Dumitru‐Claudiu , Autschbach Jochen 
Published: January 2020

AbstractRelativistic multireference ab initio wave function calculations with the restricted active space second‐order perturbation theory (RASPT2) were performed on thorocene and uranocene to determine the actinide N4,5‐edge and carbon K‐edge X‐ray absorption near‐edge structure (XANES) intensities and the metal–ligand orbital mixing in the ground state and core‐excited states. Calculated spectral intensities show very good agreement with the experiments and therefore allow detailed and unambiguous assignment of the observed spectral features. φ‐type covalent bonding or antibonding interactions are observed for thorocene in the core‐excited states, though not in the ground state. This is because the molecular orbital of φ symmetry, which is the in‐phase combination of the ligand Lφ and the Th 5fφ orbitals, can be populated with electrons in core‐excited states, whereas it is essentially unoccupied in the ground state. For uranocene, the XANES spectra do not reveal much information beyond multiplet broadening, despite the presence of distinct peaks in the spectra. Every core‐excited peak is best characterized by its own set of bond orbitals, as the excited state covalency is clearly different from the ground state covalency.
Synthesis and Systematic Structural Analysis of Cationic Half‐Sandwich Ruthenium Chalcogenocarbonyl Complexes
Authors: Suzuki Ayumi , Mutoh Yuichiro , Tsuchida Noriko , Fung Chi‐Wai , Kikkawa Shoko , Azumaya Isao , Saito Shinichi 
Published: January 2020

AbstractAlthough the chemistry of transition‐metal complexes with carbonyl (CO) and thiocarbonyl (CS) ligands has been well developed, their heavier analogues, namely selenocarbonyl (CSe) and tellurocarbonyl (CTe) complexes remain scarce. The limited availability of such CSe and CTe complexes has so far hampered our understanding of the differences between such chalcogenocarbonyl (CE: E=O, S, Se, Te) ligands. Herein, we report the synthesis and properties of a series of cationic half‐sandwich ruthenium CE complexes of the type [CpRu(CE)(H2IMes)(CNCH2Ts)][BArF4] (Cp=η5‐C5H5−; H2IMes=1,3‐dimesitylimidazolin‐2‐ylidene; ArF=3,5‐(CF3)2C6H3). A combination of X‐ray diffraction analyses, NMR spectroscopic analyses, and DFT calculations revealed an increasing π‐accepting ability of the CE ligands in the order O<S<Se<Te. A variable‐temperature NMR analysis of the thus obtained chiral‐at‐metal CE complexes indicated high stereochemical stability.
Luminescence‐Driven Electronic Structure Determination in a Textbook Dimeric DyIII‐Based Single‐Molecule Magnet
Authors: Guettas Djamila , Gendron Frédéric , Fernandez Garcia Guglielmo , Riobé François , Roisnel Thierry , Maury Olivier , Pilet Guillaume , Cador Olivier , Le Guennic Boris 
Published: January 2020

AbstractA textbook dysprosium dinuclear complex based on acetylacetone ligands, [Dy2(acac)4(μ2‐acac)2(H2O)2], has been synthesized and fully characterized. This simple dimeric lanthanide complex shows well‐resolved solid‐state luminescence and behaves as a single‐molecule magnet under zero DC field. A seminal crystal‐field approach is used to marry both magnetism and luminescence in the frame of an energetic picture.
Trigonostemons G and H, dinorditerpenoid dimers with axially chiral biaryl linkage from Trigonostemon chinensis
Authors: Zhu Qin , Tang Chunping , Mándi Attila , Kurtán Tibor , Ye Yang 
Published: January 2020

AbstractTrigonostemons G and H, two novel dimeric dinorditerpenoids, were isolated from the stem barks of Trigonostemon chinensis. Their planar structures and relative configurations were established by extensive analysis of spectroscopic data. Trigonostemons G and H possess a homodimeric biaryl skeleton obtained from two rearranged chiral nonracemic abietane‐type dinorditerpenes through an axially chiral biaryl 11,11′‐linkage. Torsional scan and computation of the transition states were carried out to estimate the rotational energy barrier, and the axial chirality (aS) was determined by time‐dependent density functional theory (TDDFT) electronic circular dichroism (ECD) calculations. The positive n‐π* ECD transitions of the isolated carbonyl chromophore above 300 nm could be used to determine the central chirality of trigonostemon G independently by ECD calculations of the diastereomers.
Detection of HER2+ Breast Cancer Cells using Bioinspired DNA‐Based Signal Amplification
Authors: Rafiee Sarah D. , Kocabey Samet , Mayer Michael , List Jonathan , Rüegg Curzio 
Published: January 2020

AbstractCirculating tumor cells (CTC) are promising biomarkers for metastatic cancer detection and monitoring progression. However, detection of CTCs remains challenging due to their low frequency and heterogeneity. Herein, we report a bioinspired approach to detect individual cancer cells, based on a signal amplification cascade using a programmable DNA hybridization chain reaction (HCR) circuit. We applied this approach to detect HER2+ cancer cells using the anti‐HER2 antibody (trastuzumab) coupled to initiator DNA eliciting a HCR cascade that leads to a fluorescent signal at the cell surface. At 4 °C, this HCR detection scheme resulted in highly efficient, specific and sensitive signal amplification of the DNA hairpins specifically on the membrane of the HER2+ cells in a background of HER2− cells and peripheral blood leukocytes, which remained almost non‐fluorescent. The results indicate that this system offers a new strategy that may be further developed toward an in vitro diagnostic platform for the sensitive and efficient detection of CTC.
Global impedance attenuation network for multistage OTAs driving a broad range of load capacitor
Authors: Aminzadeh Hamed , Valinezhad Mohammad Mahdi , Grasso Alfio Dario 
Published: January 2020

SummaryAdvanced multistage amplifiers suffer from load‐dependent stability issues, which limit the load capacitor range they can drive. In this work, the concept of global impedance attenuation (GIA) network is introduced to improve an amplifier's stability in the presence of significant load capacitor variations. Composed of multiple parallel resistor‐capacitor (RC) branches, the equivalent high‐frequency output impedance of gain stages is shaped by the GIA network such that a desired frequency spectrum is obtained over a wide range of load capacitor. The parasitic poles at the output of the gain stages are nullified by the proposed network, thereby simplifying the amplifier's transfer function and reducing the minimum load capacitor it can drive. The idea is applied to design a three‐stage operational transconductance amplifier (OTA) with cascode global impedance attenuation (CGIA). Small‐signal analysis shows that the OTA is stable regardless of the load capacitor, and it can drive very small to ultra‐large load capacitors. This feature is verified by the post‐layout simulations of a CGIA amplifier in 0.18‐μm complementary metal‐oxide semiconductor (CMOS) process. The core occupies a die area of 0.0053 mm2 while consuming a static current of 10.97 μA from 1.8‐V voltage supply. The unity‐feedback configuration is unconditionally stable for any load capacitor higher than 10 pF. To the best of our knowledge, this corresponds to the widest range of load capacitance reported for prior‐art three‐stage amplifiers.
Coverage and connectivity aware critical target monitoring for wireless sensor networks: Novel NSGA‐II–based approach
Authors: Harizan Subash , Kuila Pratyay 
Published: January 2020

SummaryAs sensor nodes have limited sensing and transmission capability, their efficient deployment takes an important role in proper monitoring of the critical targets in various applications of wireless sensor networks (WSNs). The key issues that need to be taken care during deployment are the lesser number of deployed sensors, coverage of the targets, and connectivity between the sensor nodes. In this paper, we have proposed NSGA‐II with modified dominance to solve the node deployment problem with the aforementioned three conflicting objectives. The conventional domination method is modified for better performance of the NSGA‐II. An intelligent representation of chromosome is provided. Three conflicting objectives are derived to evaluate the chromosomes. Extensive simulation on the proposed algorithm and the statistical test, and analysis of variance (ANOVA) followed by post hoc analysis are performed.
Ossifying fibromyxoid tumor: Fine‐needle aspiration cytology findings of a rare soft tissue neoplasm
Authors: Kumari Neha , Anthony Michael L. , Durgapal Prashant , Joshi Prashant P. , Rajput Deepak , Singh Ashok 
Published: January 2020

AbstractOssifying fibromyxoid tumors (OFMTs) are rare tumors of uncertain origin and intermediate (rarely metastasizing) biologic potential, with characteristic morphology of an encapsulated tumor containing polygonal cells in an abundant fibromyxoid matrix surrounded by a peripheral layer of metaplastic lamellar bone. FNA cytology of OFMT has not been sufficiently reported and till date cytological features of only seven cases have been reported so far. We report another case of OFMT in a 55‐year‐old female presenting with longstanding swelling in thigh. On fine‐needle aspiration, a tumor with moderate cellularity and myxoid areas was seen; coupled with compatible radiological findings, a diagnosis of OFMT was suggested. Establishing a correct diagnosis is important as although OFMT is benign in nature, local recurrences or rarely distant metastasis have been seen of this tumor. Hence, a high degree of suspicion with radiological correlation is of utmost importance for identification of this entity.
Studies of athlete biological passport biomarkers and clinical parameters in male and female users of anabolic androgenic steroids and other doping agents
Authors: Börjesson Annica , Lehtihet Mikael , Andersson Alexander , Dahl Marja‐Liisa , Vicente Veronica , Ericsson Magnus , Ekström Lena 
Published: January 2020

AbstractThe use of anabolic androgenic steroids (AAS) and other performance enhancing substances can change over time, so there is a need to constantly update what substances are used and can be detected. Six women and 30 men anabolic androgenic steroid users were recruited who filled out an anonymous questionnaire about their use of performance enhancing substances during the past year. Sampling took place on a single occasion and included blood and urine collection. Our aim was to identify which doping agents can be detected in men and women self‐reporting AAS use. The first choice of substances differed between men (testosterone) and women (oxandrolone). The use of growth hormones was reported among men (10%) and women (50%). Growth hormone releasing factors/secretagogs were reported by about ~ 20% in both genders. Nandrolone was the most frequently detected anabolic androgenic steroid even in those who did not report use in the past year. Of the current male testosterone users, 82% exhibited testosterone/epitestosterone (T/E) ratios of > 4. Men with current testosterone use displayed 4‐fold and 6‐fold higher median T/E, respectively, when compared with recent and previous testosterone users (P = 0.0001). Dermal testosterone use in women (n = 2) was not associated with a T/E ratio of > 4, but with supra‐physiological total serum testosterone concentrations. Changes in gonadotropins and hematological parameters were associated with the time of the last anabolic androgenic steroid intake in men, whereas in women these biomarkers were within the normal range. This highlights gender specific differences and indicates the need for additional biomarkers in female athletes.
Normal development in Ambystoma mexicanum: A complementary staging table for the skull based on Alizarin red S staining
Authors: Atkins Jade B. , Houle Laurent , Cantelon Alanna S. , Maddin Hillary C. 
Published: January 2020

AbstractBackgroundAs the role of Ambystoma mexicanum, or the Mexican axolotl, expands in research applications beyond its traditional use in studies of limb regeneration, a staging table that is more anatomically extensive is required. Here, we describe axolotl skull development as it relates to previously established developmental stages that were based on limb development.ResultsWe find that most key developmental events in the skull correspond to these previously established stages, creating easily recognizable stages of axolotl throughout skull morphogenesis.ConclusionsWith this complementary staging table in hand, researchers can stage axolotl larvae when limb data are missing or incomplete, or when cranial data alone is available.