bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of the Postoperative Outcomes of Posterior Layer Advancement and Modified Iliff Suturing to Correct Involutional Lower Lid Entropion
Authors: Kinoshita Shinsuke , Ukyo Hisaki , Masuda Naonori , Osawa Shunsuke 
Published: December 2020

Abstract
Involutional lower lid entropion is treated surgically with either incision or rotational suturing. The authors have obtained good results with the modified Iliff suture (MIS) procedure. Here, the authors compare the efficacy and postoperative outcomes of MIS and posterior layer advancement. This study included Japanese patients with a normal snap-back test, who underwent primary surgery for correcting involutional lower lid entropion, performed by the same surgeon, from April 2009 to March 2017, and who were followed up for at least 24 months postoperatively. Posterior layer advancement was performed on 50 eyelids of 48 patients (19 men, 29 women; mean age 74.1 ± 8.7 years) and MIS was performed on 50 eyelids of 40 patients (16 men, 24 women; mean age 77.3 ± 7.5 years). The authors compared the postoperative follow-up duration, recurrence rate, and operating time between groups. Postoperative follow-up duration was 34.5 ± 7.4 months in the posterior layer advancement and 32.3 ± 8.1 months in the MIS group (P = 0.180). Recurrence rate was 6% (3 cases) in the posterior layer advancement group and 4% (2 cases) in the MIS group (P > 0.999). Operating time was significantly shorter in the MIS group (5.8 ± 1.1 minutes) than in the posterior layer advancement group (31.6 ± 4.1 minutes, P < 0.001). Modified Iliff suture is less invasive than posterior layer advancement and has similar postoperative outcomes; thus, it is useful for treating involutional lower lid entropion.
Navigation-Assisted Resection and Fibula Free-Flap Reconstruction of an Extensive Maxillary Tumor
Authors: Zavattero Emanuele , Ramieri Guglielmo , Volpe Fabio , Borbon Claudia 
Published: December 2020

Objective:
Tumor resection and reconstruction as a one-step procedure requires accurate definition of the intended safety margins, precise location of osteotomy lines and reliable individual rehabilitation.
In recent years, the role of image-guided surgery in the maxillofacial region has increased significantly. As this technology allows the surgeon to track the actual position of each instrument during the operation in real-time, it makes it possible to perform extensive bone structure resections and reconstructions in anatomically distorted or complex areas, such as the head and neck region, without unnecessarily damaging vital structures.
The authors described a case of a 26-year-old woman presented to our Clinic with an extensive maxillary squamous cell carcinoma involving the infratemporal fossa. The patient underwent single-step navigation-guided en bloc resection of the tumor and defect reconstruction.
The aim of this study is describe and discuss the unusual surgical planning and the challenging operative technique adding a new case to the currently limited scientific literature on the computer-assisted head and neck oncologic surgery.
Model Experimental Study of Man-Machine Interactive Robot-Assisted Craniotomy
Authors: Aung Zin Mar , Chen Xiaojun , Xu Cheng , Sun Mengzhe , Lin Li , Xie Le , Xu Haisong , Han Dong , Chai Gang 
Published: December 2020

Abstract
To evaluate the feasibility, safety, and accuracy of the new man-machine interactive robotic system in model experiment. The implantation of the 8 to 10 bone screws over the skull model obtained from real patient's digital imaging and communications in medicine (DICOM), three-dimensional spiral computed tomography (CT) scans were taken. The end of the robotic arm was replaced with standard parts (including marker ball) for cone beam computed tomography (CBCT) scanning. The marker ball and marker pin were segmented and marked and exported via txt format. The position of the robotic end and model was obtained through the conversion of spacious position of standard parts and the executive end was replaced eventually. The water balloon was placed inside the skull model to imitate the dura mater and the destruction was documented for the system's safety. The system accuracy was evaluated by the error between the actual drilling position and the virtual plan and selection of 14 points of the skull window of milling pathway, monitored intraoperatively via Micron Tracker system and the overall skull window overlapped percentage via Mimics. Five model experiments were successfully performed with the average registration time of 3 minutes without destruction of balloon. The error of the outer table was 0.85 ± 0.45 mm, the inner table was 0.78 ± 0.49 mm, the line segment error of milling cutter was 0.93 ± 0.50 mm and the overall skull window overlapped percentage was 97.37% ± 0.78%. The system shows safety, accuracy, and reliability which can be an optional assistant method for craniotomy in the future.
Predicting Trainee Clinical Success From Performance at Simulated Endotracheal Intubation
Authors: Hastings Randolph H. , Tapia Viridiana J. , Lurie Benjamin , Hallare Jericho A. , Dinescu A. Teodora , Gabriel Rodney A. , Delson Nathan , Suresh Preetham J. 
Published: December 2020

Introduction
Multiple attempts and failure at endotracheal intubation (ETI) are common for inexperienced practitioners and can cause patient morbidity. A test to predict a provider's likelihood of success at patient ETI could assist decisions about training. This project investigated whether trainees' performance at laryngoscopy on airway mannequins predicted their laryngoscopy outcomes in patients.

Methods
Twenty-one consenting first-year anesthesiology residents, emergency medicine residents, and medical students enrolled in this prospective, observational study. They performed laryngoscopy and ETI with a curved laryngoscope on 4 airway mannequins. Metrics included peak dental force, procedure duration, esophageal intubation, laryngeal view, and first-pass ETI success on the mannequins. Trainee data from 203 patient ETIs were collected over a roughly 2-month period centered around the simulation test. Multivariable logistic regression analyzed the relationship of mannequin metrics, participant experience, and a patient difficult airway score with trainee ETI outcomes in patients.

Results
Median trainee first-pass success rate at patient ETI was 63%, the rate of ETI problems was 16%, and the esophageal intubation rate was 6%. Laryngoscopy peak dental force, first-pass ETI success, and duration on individual mannequins were significant predictors of patient ETI first-pass success. Metrics from 2 of the 4 mannequins predicted ETI problems.

Discussion
Performance metrics from simulated laryngoscopy predicted trainee outcomes during patient ETI. First-pass success and ETI problems affect patient safety and are related to trainee skill. Mannequin laryngoscopy tests could identify trainees who would benefit from additional practice. The metrics could be surrogate end points in research to optimize simulated laryngoscopy training.
Co-constructive Patient Simulation
Authors: Martin Andrés , Weller Indigo , Amsalem Doron , Duvivier Robbert , Jaarsma Debbie , de Carvalho Filho Marco Antonio 
Published: December 2020

Introduction
In simulation sessions using standardized patients (SPs), it is the instructors, rather than the learners, who traditionally identify learning goals. We describe co-constructive patient simulation (CCPS), an experiential method in which learners address self-identified goals.

Methods
In CCPS, a designated learner creates a case script based on a challenging clinical encounter. The script is then shared with an actor who is experienced working as an SP in medical settings. An instructor with experience in the model is involved in creating, editing, and practicing role play of the case. After co-creation of the case, learners with no prior knowledge of the case (peers or a supervisor) interview the SP. The clinical encounter is followed by a group debriefing session.

Results
We conducted 6 CCPS sessions with senior trainees in child and adolescent psychiatry. Topics that are difficult to openly talk about may be especially appropriate for the CCPS model—without overt guidance or solicitation, the scripts developed by learners for this series involved: medical errors and error disclosure; racial tensions, including overt racism; interprofessional conflict; transphobia; patient-on-provider violence; sexual health; and the sharing of vulnerability and personal imperfections in the clinical setting.

Conclusions
Co-constructive patient simulation provides an alternative multistage and multimodal approach to traditional SP simulation sessions that can adapt iteratively and in real time to new clinical vicissitudes and challenges This learner-centered model holds promise to enrich simulation-based education by fostering autonomous, meaningful, and relevant experiences that are in alignment with trainees' self-identified learning goals.
Safety Considerations for In Situ Simulation in Closed SARS-CoV-2 Units
Authors: Jafri Farrukh N. , Shah Sharan , Yang Christina J. , Restivo Andrew , Singh Maninder , Yoon Andrew , Ahmed Sadia T. 
Published: December 2020

Summary Statement
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic placed a tremendous strain on the healthcare system, which led to the deployment of new personnel into acute care settings, early graduation of medical students, and development of new treatment spaces. Education teams at the Montefiore Health System and New York Health and Hospitals/Jacobi Medical Center found simulation, both laboratory-based and in situ, critical to the training of medical staff and investigation of latent safety threats. Through our experience, we encountered unique infection control concerns based on in situ sessions, which prompted us to redesign our programs for the treatment of SARS-CoV-2. Using this experience, we outline our rationale for the use of in situ simulation for newly developed SARS-CoV-2 spaces along with recommendations on safety checks to consider before starting.
Pleurectomy Decortication in the Treatment of Malignant Pleural Mesothelioma
Authors: Lapidot Moshe , Gill Ritu R. , Mazzola Emanuele , Freyaldenhoven Samuel , Swanson Scott J. , Jaklitsch Michael T. , Sugarbaker David J. , Bueno Raphael 
Published: December 2020

Objective:
We report a series of 355 consecutive patients treated over 9 years in a single institution with intended PDC.

Background:
Surgery for MPM has shifted from extra-pleural pneumonectomy to PDC with the goal of MCR.

Methods:
Clinical and outcome data were reviewed. Kaplan-Meier estimators and log rank test were used to compare the overall survival, and logistic regression models were used.

Results:
MCR was achieved in 304. There were 223 males, median age was 69 and histology was epithelioid in 184. The 30 and 90-day mortality were 3.0% and 4.6%.
Most complications were low grade. Prolonged air leak in 141, deep venous thrombosis in 64, Atrial fibrillation in 42, chylothorax in 24, Empyema in 23, pneumonia in 21, Hemothorax in 12 and pulmonary embolus in 8. Median/5-year survival were 20.7 months/17.9% in the intent-to-treat cohort and 23.2months/21.2% in the MCR group. The survivals were best for patients with Tlstage and epithelioid histology (69.8months/54.1%). In a multivariable analysis, factors that were found to be associated with longer patient overall survival included epithelioid histology, T stage, quantitative clinical stage/tumor volume staging, adjuvant chemotherapy, intraoperative heated chemo, female sex, and length of stay shorter than 14 days.

Conclusions:
PDC is feasible with low mortality and is associated with manageable complication rates. 5-year survival of patients undergoing PDC with MCR in multi-modality setting is approaching 25% depending on quantitative and clinical stage, sex and histological subtype and is better than PDC without- MCR.
Imaging Tolerance Induction in Neonatal Mice: Hierarchical Interplay Between Allogeneic Adult and Neonatal Immune Cells
Authors: Bascom Roger A. , Tao KeSheng , West Lori J. 
Published: December 2020

Background.
In Medawar’s murine neonatal tolerance model, injection of adult semiallogeneic lymphohematopoietic cells (spleen cells [SC] and bone marrow cells [BMC]) tolerizes the neonatal immune system. An eventual clinical application would require fully allogeneic (allo) cells, yet little is known about the complex in vivo/in situ interplay between those cells and the nonconditioned neonatal immune system.

Methods.
To this end, labeled adult SC and BMC were injected into allogeneic neonates; interactions between donor and host cells were analyzed and modulated by systematic depletion/inactivation of specific donor and host immune effector cell types.

Results.
Consistent with effector cell compositions, allo-SC and allo-SC/BMC each induced lethal acute graft-versus-host disease, whereas allo-BMC alone did so infrequently. CD8 T cells from SC inoculum appeared naïve, while those of BMC were more memory-like. Age-dependent, cell-type dominance defined the interplay between adult donor cells and the neonatal host immune system such that if the dominant adult effector type was removed, then the equivalent neonatal one became dominant. Depletion of donor/host peripheral T cells protected against acute graft-versus-host disease and prolonged heart allograft survival; peripheral CD8 T-cell depletion together with CD4 T cell–costimulation blockade induced more robust tolerance.

Conclusions.
This comprehensive study provides direct observation of the cellular interplay between allogeneic donor and host immune systems, adds to our previous work with semiallogeneic donor cells, and provides important insights for robust tolerance induction. Induction of transplant tolerance in neonates will likely require “crowd sourcing” of multiple tolerizing cell types and involve depletion of immune effector cells with costimulation blockade.
Serum Glycomics on Postoperative Day 7 Are Associated With Graft Loss Within 3 Months After Liver Transplantation Regardless of Early Allograft Dysfunction
Authors: Verhelst Xavier , Geerts Anja , Colman Roos , Vanlander Aude , Degroote Helena , Abreu de Carvalho Luis , Meuris Leander , Berrevoet Frederik , Rogiers Xavier , Callewaert Nico , Van Vlierberghe Hans 
Published: December 2020

Background.
Prediction of outcome after liver transplantation (LT) is limited by the lack of robust predictors of graft failure. In this prospective study, we aimed to define a serum glycomic signature in the first week after LT that is associated with graft loss at 3 mo after LT.

Methods.
Patients were included between January 1, 2011, and February 28, 2017. Glycomic analysis was performed using DNA sequencer-associated fluorophore-associated capillary electrophoresis on a serum sample 1 wk after LT. Making use of Lasso regression, an optimal glycomic signature was identified associated with 3-mo graft survival.

Results.
In this cohort of 131 patients, graft loss at 3 mo occurred in 14 patients (11.9%). The optimal mode, called the GlycoTransplantTest, yielded an area under the curve of 0.95 for association with graft loss at 3 mo. Using an optimized cutoff for this biomarker, sensitivity was 86% and specificity 89%. Negative predictive value was 98%. Odds ratio for graft loss at 3 mo was 70.211 (P < 0.001; 95% confidence interval, 10.876-453.231).

Conclusions.
A serum glycomic signature is highly associated with graft loss at 3 mo. It could support decision making in early retransplantation.
Real-world Site Experiences With GeneMatch
Authors: Bardach Shoshana H. , Langbaum Jessica B. , Kebodeaux Carey S. , Jicha Gregory A. 
Published: December 2020

Background:
Registries have been proposed as a novel way to accelerate targeted recruitment for Alzheimer disease prevention clinical trials. However, there are limited data regarding registry effectiveness at accelerating recruitment and enrollment in research opportunities. This manuscript explores one site’s experience with GeneMatch, a novel genetic registry for Alzheimer disease research.

Methods:
Referrals from GeneMatch to the site were tracked to understand the demographics of those referred and ultimate research enrollment outcomes. Referrals were cross-referenced with the site’s existing recruitment database, to better understand the role of GeneMatch in the context of existing recruitment efforts.

Results:
GeneMatch referred 86 individuals to the site, resulting in 54 individuals coming into the site to pursue research involvement further. The majority of referrals (52/86, 60.47%) did not have prior contact with the site about research engagement, and having prior site contact did not significantly relate to engaging in on-site research.

Conclusions:
GeneMatch helped identify new individuals for participation in Alzheimer disease prevention studies. Results highlight the value of continuing local site-level efforts while also taking advantage of registries to enhance research recruitment. Ongoing efforts to further develop these and other novel strategies for outreach and engagement are much needed.
Clinical Characteristics and Risk Factors for Myocardial Injury and Arrhythmia in COVID-19 patients
Authors: Ren Hong Gang , Guo Xingyi , Tu Lei , Hu Qinyong , Blighe Kevin , Safdar Luqman Bin , Stebbing Justin , Weiner Shepard D , Willis Monte S , Rosendaal Frits R , Xu Guogang , Cao Feng , Wang Dao Weng 
Published: December 2020

AbstractThe authors have withdrawn this manuscript because of a lack of novelty of results and, with that, a lack of intention to publish in a peer reviewed journal. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.
Razor: annotation of signal peptides from toxins
Authors: Bhandari Bikash K. , Gardner Paul P. , Lim Chun Shen 
Published: December 2020

ABSTRACTMotivationSignal peptides are responsible for protein transport and secretion and are ubiquitous to all forms of life. The annotation of signal peptides is important for understanding protein translocation and toxin secretion and evolution.ResultsHere we explore the features of these signal sequences from eukaryotic proteins. Strikingly, we find that the signal peptides from secretory toxins have common features across kingdoms, supporting the idea of horizontal gene transfer or convergence of toxin genes across kingdoms. We leverage these features to build Razor, a simple yet powerful tool specialised in identifying signal peptides from toxins using the first 23 N-terminal residues. We demonstrate the usability of Razor by analysing all the sequences reviewed by UniProt. Indeed, Razor is able to identify toxins using their N-terminal sequences only. Strikingly, we also discover that many defensive proteins across kingdoms harbour a toxin-like signal peptide; some of these defensive proteins have emerged through convergent evolution, e.g. defensin and defensin-like protein families, and phospholipase families. In sum, Razor uses an approach independent of homology search to identify novel and known toxin classes across species using N-terminal residues.Availability and implementationRazor is available as a web application (https://tisigner.com/razor) and a command-line tool (https://github.com/Gardner-BinfLab/Razor).
To the canopy and beyond: Air samples reveal wind dispersal as a driver of ubiquitous protistan pathogen assembly in tree canopies
Authors: Jauss Robin-Tobias , Nowack Anne , Walden Susanne , Wolf Ronny , Schaffer Stefan , Schellbach Barbara , Bonkowski Michael , Schlegel Martin 
Published: December 2020

AbstractWe analyzed air dispersal of the protistan phyla Cercozoa and Oomycota with an air sampler near the ground (~2 m) and in tree crowns (~25 m) of three tree species (oak, linden and ash) in a temperate floodplain forest in March (before leafing) and May (after leaf unfolding) with a cultivation-independent high throughput metabarcoding approach. Both, Cercozoa and Oomycota, contain important pathogens of forest trees and other vegetation. We found a high diversity of Cercozoa and Oomycota in air samples with 122 and 81 OTUs, respectively. Especially oomycetes showed a high temporal variation in beta diversity between both sampling dates. Differences in community composition between air samples in tree canopies and close to the ground were however negligible, and also tree species identity did not affect communities in air samples, indicating that the distribution of protistan propagules through the air was not spatially restricted in the forest ecosystem. OTUs of plant pathogens, whose host species that did not occur in the forest, demonstrate wind dispersal of propagules from outside the forest biome. Overall, our results lead to a better understanding of the stochastic processes of wind dispersal of protists and protistan pathogens, a prerequisite to understand the mechanisms of their community assembly in forest ecosystems.ImportanceWind dispersal has been shown to play a crucial role in protistan community assembly. The protistan taxa Cercozoa and Oomycota contain important plant parasites with a major ecologic and economic impact. However, comprehensive assessments of cercozoan and oomycete diversity in forest air samples were lacking. Using a cultivation-independent high throughput metabarcoding approach, we analyzed cercozoan and oomycete air dispersal in forest floors and the canopy region – a potential filter for microbial propagules. Our study provides insights into the diversity and community assembly of protists within the air, contributing to a better understanding which factors drive the distribution of plant pathogens within forest ecosystems.
A Novel COVID-19 Early Warning Tool: Moore Swab Method for Wastewater Surveillance at an Institutional Level
Authors: Liu Pengbo , Ibaraki Makoto , VanTassell Jamie , Geith Kelly , Cavallo Matthew , Kann Rebecca , Moe Christine 
Published: December 2020

ABSTRACTSARS-CoV-2 is a respiratory virus but it is also detected in a significant proportion of fecal samples of COVID-19 cases. Recent studies have shown that wastewater surveillance can be a low-cost tool for management of COVID-19 pandemic and tracking COVID-19 outbreaks in communities but most studies have been focusing on sampling from wastewater treatment plants. Institutional level of wastewater surveillance may serve well for early warning purposes since cases can be tracked and immediate action can be executed in the event of positive signal. In this study, a novel Moore swab method was developed and used for wastewater surveillance of COVID-19 at institutional level. Among the 219 swab samples tested, 28 (12.8%) swabs collected from the three campuses and two buildings were positive for SARS-CoV-2. Further individual clinical diagnosis validated the wastewater results and indicated that this method was sensitive enough to detect 1-2 cases in a building. In addition, comparison between grab and Moore swab methods from the hospital sewage line indicated that Moore swab method was more sensitive than the grab sampling method. These results suggest that the Moore swab is a sensitive, practical, and easy to use early warning tool for COVID-19 surveillance especially in low-resource settings and at an early stage of infection in communities.