bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brilliant Blue G, a P2X7 receptor antagonist, attenuates early phase of renal inflammation, interstitial fibrosis and is associated with renal cell proliferation in ureteral obstruction in rats
Authors: Pereira José Monteiro Sad , Barreira André Luis , Gomes Conrado Rodrigues , Ornellas Felipe Mateus , Ornellas Débora Santos , Miranda Luiz Carlos , Cardoso Lucio Ronaldo , Coutinho-Silva Robson , Schanaider Alberto , Morales Marcelo M. , Leite Maurilo , Takiya Christina Maeda 
Published: November 2019

Abstract
Background Previous study showed that purinergic P2X7 receptors (P2X7R) reach the highest expression in the first week after unilateral ureteral obstruction (UUO) in mice, and are involved in the process of inflammation, apoptosis and fibrosis of renal tissue. We, herein, document the role of purinergic P2X7 receptors activation on the third day of UUO, as assessed by means of BBG as its selective inhibitor.Methods We investigated the effects of brilliant blue G (BBG), a P2X7R antagonist, in the third day of kidney tissue response to UUO in rats. For this purpose, male Wistar rats submitted to UUO or sham operated, received BBG or vehicle (V), comprising four groups: UUO-BBG, UUO-V, sham-BBG and sham-V. The kidneys were harvested on day 3 UUO and prepared for histology, immunohistochemistry (P2X7R, PCNA, CD-68, α-sma, TGF-β1, Heat-shock protein-47, TUNEL assay), quantitative real-time PCR (IL-1β, procollagens type I, III, and IV) for mRNA quantification.Results The group UUO-V presented an enhancement in tubular cell P2X7-R expression, increase influx of macrophages and myofibroblasts, HSP-47 and TGF- β1 expression. Also, upregulation of procollagen types I, III, and IV, and IL-1β mRNAs were seen. On the other hand, group UUO-BBG showed lower expression of procollagens and IL-1β mRNAs, as well as less immunoreactivity of HSP-47, TGF-β, macrophages, myofibroblasts, and tubular apoptosis. This group also presented increased epithelial cell proliferation.Conclusion BBG, a known highly selective inhibitor of P2X7R, attenuated renal inflammation, collagen synthesis, renal cell apoptosis, and enhanced renal cell proliferation in the early phase of rat model of UUO.
Revascularization for Stable Chronic Total Occlusion is Essential for Diabetic Patient with Heart Failure
Authors: Yan Yunfeng , Zhang Mingduo , Ma Zhuo , Zhang Zilong , Yuan Fei , Liu Hong , Xu Feng , Zhang Min , Wang Wei , Zhou Kuo , Zhao Quanming , Lyu Shuzheng 
Published: November 2019

Abstract
Background Revascularization is the recommended treatment strategy for patients with heart failure (HF) and coronary artery disease (CAD). However, chronic total occlusion (CTO) is less attempt. Furthermore, there were conflicting debates on if diabetic HF patients gained benefits from revascularization. As to CTO revascularization, no study answered if it offered benefits to diabetic HF patients. Methods Diabetic patients with stable CTO and HF were consecutively enrolled in this retrospective cohort study. Based on treatment strategies to the CTO vessel, patients were assigned to successful revascularization (CTO-SR) or medical therapy (CTO-MT) group. The primary endpoints were major adverse cardiac events (MACE). Subgroup analysis were performed based on left ventricular ejection fraction (LVEF) and relevant baseline variables. Results A population of 680 patients were enrolled in the present study: 344 patients in the CTO-MT group, and 336 patients in the CTO-SR group. After a median follow-up of 34 months, CTO-SR was superior to CTO-MT in MACE (adjusted hazard ratio [HR]: 0.462, 95% conference interval [CI]: 0.337-0.634), which could mainly due to the superiority in cardiac-death and TVR. Propensity matching analysis also confirmed CTO-SR’s superiority (HR: 0.494 [0.337-0.725]). Subgroup analysis further confirmed a consistent superiority in patients with LVEF≥40%, but not in those with LVEF<40%.Conclusions For patients with diabetes, HF and stable CTO, CTO-SR was superior to CTO-MT. CTO-SR’s superiority was consistent in patients with LVEF≥40%, but not for patients with LVEF<40%. Trial registration This study was not registered in an open access database.
Downregulation of S100 Calcium Binding Protein A12 inhibits the growth of glioma cells
Authors: Lu Chunhe , Liu Jia , Yao Mingze , Li Lun , Li Guangyu 
Published: November 2019

Abstract
Introduction: S100 Calcium Binding Protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. Methods: In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of s100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by ShRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT,clony formation assay, transwell assay ,flow cytometry assa and westernblot. Results: We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT. Conclusions: These findings demonstrated a novel function for S100A12 in glioma progression and suggested that S100A12 may be served as a new marker in the tumorigenesis and progression of glioma.
Peptides regulating gastrointestinal function in children before and after hematopoietic stem cell transplantation
Authors: Rej Magdalena , Skoczeń Szymon , Pietrys Danuta , Kwiecińska Kinga , Tomasik Przemysław J. , Wójcik Małgorzata , Strojny Wojciech , Dłużniewska Agnieszka , Klimasz Katarzyna , Fijorek Kamil , Korostyński Michał , Piechota Marcin , Balwierz Walentyna 
Published: November 2019

Abstract
Gastrointestinal tract function and it's integrity are controlled by a number of peptides whose secretion is influenced by severe inflammation. In stomach the main regulatory peptide is ghrelin. For upper small intestine cholecystokinin and glucagon-like peptide- 1 are secreted, while fibroblast growth factor-21 is secreted by several organs, including the liver, pancreas, and adipose tissue [12]. Hematopoietic stem cell transplantation causes serious mucosal damage, which can reflect on this peptides. The aim of the study was to determine fasting plasma concentrations of ghrelin, cholecystokinin, glucagone- like peptide-1, and fibroblast growth factor -21, and their gene expressions, before and 6 months after hematopoietic stem cell transplantation. 27 children were studied. C ontrol group included 26 healthy children. Acute graft versus host disease was diagnosed in 11 patients (41%, n=27). Median pre-transplantation concentrations of ghrelin, cholecystokinin , and glucagone like peptide-1, as well as their gene expressions, were significantly lower compared with the control group. In contrast, median fibroblast growth factor-21 concentrations were near-significantly higher before stem cell transplantation than in the control group. A comparison of pre- and post-transplantation groups revealed significantly higher concentrations of the studied peptides (except fibroblast growth factor-21) and respective gene expressions in the post–hematopoietic transplant group. M edian glucagone like peptide-1 concentrations were significantly decreased in patients with features of acute graft versus host disease. Moreover, negative correlation between glucagone like peptide-1 concentrations and acute graft versus host disease severity was found. Increased concentrations and gene expressions of gastrointestinal tract regulation peptides can be caused by stimulation of regeneration in the severe injured organ. Measurement of these parameters may be a useful method of assessment of severity of gastrointestinal tract complications of hematopoietic stem cell transplantation.
Clinical characteristics and risk factors of 47 cases with ruptured neuroblastoma in children
Authors: Qin Hong , Yang Shen , Cai Siyu , Ren Qinghua , Han Wei , Yang Wei , Cheng Haiyan , Ma Xiaoli , Wang Huanmin 
Published: November 2019

Abstract
Background: Neuroblastoma (NB) tumor rupture was a rare oncology emergency with poor prognosis. We aimed to evaluate clinical characteristics and risk factors for ruptured NB.
Methods: A retrospective study was conducted on 47 confirmed ruptured NB patients in Beijing Children's Hospital between January 2009 and January 2019. To identify tumor rupture risk factors within high-risk NB, we included 93 consecutive nonruptured high-risk NB patients from January 2017 to January 2019.
Results: Median age at presentation was 29 months (adrenal and retroperitoneum origin) for 47 ruptured NB patients. Spontaneous tumor rupture occurred in 22 cases; 18 developed tumor rupture during or after the first chemotherapy cycle, and 7 developed after core needle biopsy. Five patients died of tumor rupture, and 17 patients’ parents refused further anti-tumor therapy. Among 25 remaining patients, 6 survived without disease, 5 remained under treatment with stable disease, and 14 died. According to multivariate logistic regression analysis, the maximum diameter of primary tumor > 13.20 cm and MYCN gene amplification were independent risk factors for tumor rupture within high-risk NB.
Conclusions: Tumor rupture is an uncommon, life-threatening presentation among NB patients; these patients are most likely to have poor outcomes due to tumor recurrence or rapid progression. Several treatment modalities, including symptomatic support therapy and chemotherapy, are important for saving lives and creating conditions for subsequent treatment.
Genome-wide identification and expression analysis of the GhIQD gene family in upland cotton (Gossypium hirsutum L.)
Authors: Dou Lingling , Lv Limin , Li Huaizhu , Li Yuhong , Xi Ruimin , Zhao Qin , Wang Wenbo , Pang Chaoyou , Zou Changsong , Song Guoli , Xiao Guanghui 
Published: November 2019

Abstract
Background: Calmodulin (CaM) is one of the most important Ca 2+ signaling receptors because it regulates diverse physiological and biochemical reactions in plants. CaM functions by interacting with CaM-binding proteins (CaMBPs) to modulate Ca 2+ signaling. IQ domain (IQD) proteins are plant-specific CaMBPs that bind to CaM by its specific CaM binding sites.
Result: In this study, we firstly identified 102 GhIQD genes in the Gossypium hirsutum L. genome. The GhIQD gene family was classified into seven clusters (Ia, Ib, Ic, II, IIIa, IIIb and IV), and we then mapped the GhIQD genes to the 26 chromosomes. Moreover, we found that 100 of the 102 GhIQD genes resulted from segmental duplication events, indicating that segmental duplication is the main force driving GhIQD gene expansion. Gene expression pattern analysis showed that a total of 89 GhIQD genes are expressed in the elongation stage and second cell wall biosynthesis stage of the fiber cells, suggesting that GhIQD genes may contribute to fiber cell development in cotton. In addition, we found that 20 randomly-selected GhIQD genes were highly expressed in different tissues. Exogenous application of MeJA significantly enhanced the expression levels of GhIQD genes.
Conclusion: Our study shows that GhIQD genes are involved in fiber cell development in cotton and are also widely induced by MeJA, which provides a basis to systematically characterize the evolution and biological functions of GhIQD genes, as well as clues to breed better cotton varieties in the future.
Mean platelet volume and mean platelet volume/platelet count ratio in nonvalvular atrial fibrillation stroke and large artery atherosclerosis stroke
Authors: Zhu Ning , Hao Shu , Jiang Wenbing , Wang Yi , Zhang Shunkai 
Published: November 2019

Abstract
Objective Ischemic stroke subtypes such as patients with large artery atherosclerosis, cardioembolism and embolic stroke of undetermined source were investigated. This study was performed aimed to determine mean platelet volume (MPV) and mean platelet volume/platelet count (MPV/Plt) Ratio in nonvalvular atrial fibrillation (AF) stroke and large artery atherosclerosis (LAA) stroke.Methods We conducted a retrospective study of consecutive patients for treatment of acute ischemic stroke at Ruian People's Hospital from March 2017 to October 2018. The patients with ischemic stroke caused by AF and LAA were recruited to this study. Ischemic stroke was confirmed by MRI and MRA, ischemic lesions on diffusion-weighted imaging were measured in terms of size, composition, and pattern. MPV and platelet count were examined and (MPV/Plt) Ratio was calculated.Results 371 patients were enrolled composing of 177 (47.7%) nonvalvular AF and 194 (52.2%) with LAA. The MPV (11.3 ± 1.3 vs. 10.8 ±1.0, p﹤0.001) and MPV/Plt ratio (0.066±0.025 vs. 0.055±0.20, p﹤0.001) were much higher in AF group than LAA group. ROC analysis showed MPV (AUC: 0.624, confidence interval:0.567-0.68,p﹤0.001) and MPV/Plt (AUC: 0.657, confidence interval:0.601-0.713,p﹤0.001) predicted AF between the two groups. MPV/Plt ratio was negatively associated with lesion volume (r = -0.161, p = 0.033) in AF. The analyses of subtypes of composition of infarcts and infarct pattern showed that MPV/Plt ratio was almost higher in AF than LAA except for subcortical-only pattern. Multivariable regression analyses demonstrated NIHSS score (r = 2.74; p﹤0.001), LAD (r = -1.15; P = 0.025) and MPV/Plt ratio (r = -180.64; P = 0.021) were correlated with lesion volume.Conclusion Our results indicated elevated MPV and MPV/Plt ratio for the identification of difference between AF and LAA in patients with ischemic stroke.
Diversity of human rhinovirus species among children with severe or mild acute lower respiratory infection in Beijing, China, during 2016–2019
Authors: Chen Dongmei , Zhang Ruoxi , Qian Yuan , Sun Yu , Zhu Runan , Wang Fang , Ding Yaxin , Zhou Yutong , Guo Qi , Qu Dong , Cao Ling , Zhao Linqing 
Published: November 2019

Abstract
Background Human rhinovirus (HRV) infections were confirmed in severe acute lower respiratory infections (ALRI) . Methods To evaluate the diversity and circulation pattern of HRVs species, specimens were collected from pediatric patients with ALRI during Dec 2016 to Feb 2019 and screened by RVP FAST Assay. Specimens positive for HRVs/ Enteroviruses (EVs) were then tested for HRVs and EVs by PCR. Then the capsid protein gene of HRVs was amplified and sequenced for species identification, and clinical data of HRV infections were analyzed. The chi-square (χ 2 ) test and rank sum test was used for statistical analysis using SPSS Statistics 22.0 version. In order to test and evaluate the relationship between patients positive for different HRV species and the outcome of Intensive Care Unit (ICU) (ICU group) or Department of Respiratory admission (Respiratory group), a logistic model was constructed using multiple logistic regression analysis. Results Among 1835 specimens tested, 363 (19.8%) were positive for EVs/HRVs, and 314 (86.5%, 314/363) were positive for HRVs, including 177 (56.4%, 177/314) HRV A, 29 (9.2%, 29/314) HRV B, and 108 (34.4%, 108/314) HRV C. Using HRV C as the control species, HRV A was a dangerous factor for severe clinical outcome (OR=1.983, 95% CI=1.091-3.605) ( p =0.025). Patients positive for HRVs from ICU group were significantly younger than those from Respiratory group (0.39 years: 1.80 years, p =0.000) and age was confirmed as a protective factor for severe ALRI. In August and September, HRV A and B are the dominant species, then HRV C in October and December. Among 96 types confirmed in the study, the predominant types usually showed in no consecutive years. Conclusions HRVs were important viral pathogens for ALRI in children. HRV A and C are more popular. Compared to HRV C, HRV A were associated more powerful with severe ALRI. The risk of severe ALRI by HRVs infections is decreased with the increasing of age.
Community awareness towards cesarean section delivery and its associated factors in Enderta woreda, Tigray Ethiopia, 2017: community based mixed study design
Authors: Hadush Abeba 
Published: November 2019

Abstract
Objective: To assess community awareness towards cesarean section delivery and associated factors in Enderta woreda, Tigray, Ethiopia, 2017. When cesarean section is decided during labor, most of the women fear and refuse the decision. There are studies in some African countries assessing factors that affect women's awareness on cesarean section, but they are institutional based and quantitative only. In this research the study is at the community level and in addition to the quantitative part, it has qualitative design to explore the community feeling.Results: Three hundred eighteen (95.2%) of the study participants were aware of caesarean section. Monthly income (AOR 18.822: 95% CI 1.463-24.228: P=0.048), and educational status of the mother (AOR 12.443: 95% CI 1.025-22.905: P = 0.024) had significant association with awareness towards cesarean delivery. Most of the participants replied cesarean section saves the life of mother and fetus and the main reason for mothers to refuse in decision is they don’t know well about cesarean section.
Infection and RNA-seq analysis of a zebrafish tlr2 mutant shows a broad function of this Toll-like receptor in transcriptional and metabolic control and defense to Mycobacterium marinum infection
Authors: Hu Wanbin , Yang Shuxin , Shimada Yasuhito , Münch Magnus , Marín-Juez Rubén , Meijer Annemarie H. , Spaink Herman P. 
Published: November 2019

Abstract
Background
The function of Toll-like receptor 2 (TLR2) in host defense against pathogens, especially Mycobacterium tuberculosis (Mtb) is poorly understood. To investigate the role of TLR2 during mycobacterial infection, we analyzed the response of tlr2 zebrafish mutant larvae to infection with Mycobacterium marinum (Mm), a close relative to Mtb, as a model for tuberculosis. We measured infection phenotypes and transcriptome responses using RNA deep sequencing in mutant and control larvae.
Results
tlr2 mutant embryos at 2 dpf do not show differences in numbers of macrophages and neutrophils compared to control embryos. However, we found substantial changes in gene expression in these mutants, particularly in metabolic pathways, when compared with the heterozygote tlr2+/- control. At 4 days after Mm infection, the total bacterial burden and the presence of extracellular bacteria were higher in tlr2-/- larvae than in tlr2+/-, or tlr2+/+ larvae, whereas granuloma numbers were reduced, showing a function of Tlr2 in zebrafish host defense. RNAseq analysis of infected tlr2-/- versus tlr2+/- shows that the number of up-regulated and down-regulated genes in response to infection was greatly diminished in tlr2 mutants by at least 2 fold and 10 fold, respectively. Analysis of the transcriptome data and qPCR validation shows that Mm infection of tlr2 mutants leads to decreased mRNA levels of genes involved in inflammation and immune responses, including il1b, tnfb, cxcl11aa/ac, fosl1a, and cebpb. Furthermore, RNAseq analyses revealed that the expression of genes for Maf family transcription factors, vitamin D receptors, and Dicps proteins is altered in tlr2 mutants with or without infection. In addition, the data indicate a function of Tlr2 in the control of induction of cytokines and chemokines, such as the CXCR3-CXCL11 signaling axis.
Conclusion
The transcriptome and infection burden analyses show a function of Tlr2 as a protective factor against mycobacteria. Transcriptome analysis revealed tlr2-specific pathways involved in Mm infection, which are related to responses to Mtb infection in human macrophages. Considering its dominant function in control of transcriptional processes that govern defense responses and metabolism, the TLR2 protein can be expected to be also of importance for other infectious diseases and interactions with the microbiome.
EWING SARCOMA: FEATURES OF LYMPHOGENOUS METASTASIS AND PROGNOSTIC FACTORS
Authors: Vasilyev N. V. , Poletaeva S. V. , Tabakaev S. A. , Tyukalov Yu. I. , Perelmuter V. M. 
Published: November 2019

Background. Lymphogenous metastasis in Ewing sarcoma is a relatively rare and poorly studied event associated with aggressive clinical course and poor prognosis. Until now, no risk factors for lymphogenous metastasis in patients with Ewing sarcoma are reported.The purpose of the study was to evaluate tumor characteristics as predictors for lymphogenous metastasis and to create a mathematical model for assessing the risk of developing lymph node metastases in patients with Ewing sarcoma.Material and Methods. Clinical characteristics of the tumor were studied in 88 patients with Ewing sarcoma: in 8 patients with lymphogenous metastasis and in 80 patients having no lymphogenous metastasis. The primary tumor in all patients with lymphogenous metastasis was found to have an extraskeletal origin. Morphological and immunohistochemical characteristics of the tumor were studied in 31 patients with Ewing sarcoma: in 8 patients with lymphogenous metastasis and in 23 patients without lymphogenous metastasis.Results. Statistical analysis and comparative evaluation of the characteristics of the immunophenotype and histological pattern of the tumor in the two studied groups showed significant differences regarding several of them: the structure of nuclear crowding (fusion of nuclei), focal hemorrhages, nuclear normochromasia, and positive expression of cytokeratins by tumor cells. The above signs (except for nuclear normochromasia) were the basis for creating a mathematical model capable of predicting the risk of lymphogenous metastases in Ewing sarcoma.Conclusion. The revealed association with lymphogenous metastasis of cytokeratin expression can be considered as indirect confirmation of the pathogenetic significance of the mesenchymal-epithelial transition in the mechanism of lymphogenous metastasis.
ENCEPHALOPATHY AND HYPERLACTATEMIA AS A COMPLICATION OF 5-FLUOROURACIL TREATMENT
Authors: Kurmukov I. A. , Yunayev G. S. , Prosvirnov A. A. , Borovkova N. B. , Kashiya Sh. R. 
Published: November 2019

Background. There is a wide spectrum of metabolic and toxic disorders that can cause acute encephalopathy in cancer patients. In routine clinical practice, hypoglycemia, vitamin B1 (thiamine) deficit, fulminant liver failure, uremia, severe hypoand hypernatremia should be primarily excluded. Central neurotoxicity associated with hyperammonemia in patients receiving 5-fluorouracil (5-FU) and oral fluoropyrimidines should be considered in differential diagnosis. In this case, the analysis of the blood acid-base status and the detection of B-type hyperlactatemia can facilitate the diagnosis of the cause of encephalopathy.Case description. We present two cases of hyperlactatemia and encephalopathy in stage IV cancer patients with continuous infusion of 5-FU via a portable infusion pump.Conclusion. Diagnosis of the frequent fluoropyrimidin-related adverse effects, such as myelosuppression, anorexia, diarrhea, mucositis, and palm-plantar syndrome, are routine and mastered by an oncologist at the very beginning of his/her professional activity. Specific fluoropyrimidinerelated encephalopathy or hyperlactatemia are difficult to suspect and recognize. We hope our description will be useful to prevent possible diagnostic errors.