bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of the greenness of new stability indicating micellar UPLC and HPTLC methods for determination of tenofovir alafenamide in dosage forms
Authors: Said Noha S , Nasr Zeinab Adel , Abdel-Razeq Sawsan A 
Published: December 2020

Abstract
In this study, a green stability indicating chromatographic methods were developed and validated for the quantitative determination of tenofovir alafenamide in the presence of its degradation products in bulk powder as well as in dosage forms. The first method was micellar UPLC in which separation was achieved on kinetex ® 1.7 μm HILIC 100A, LC column using an ecofriendly micellar mobile phase consisting (0.05 M sodium dodecyl sulphate and 0.05 M sodium dihydrogen phosphate, (pH 5.5) and 10% 1-propanol (70:30) at a flow rate of 1 mL min−1 with a UV detection at 210 nm. The second method depended on HPTLC method performed on HPTLC plates pre-coated with silica gel 60 F254 using a mobile phase consisting of n-butanol—acetic acid (7:3, v/v) and detection at 260 nm. Tenofovir alafenamide was subjected to stress conditions including alkaline and acidic degradation. Beer’ law was obeyed over the concentration range of 1–18 μg mL−1 and 0.1–4 μg/spot for micellar UPLC and HPTLC methods, respectively. Both methods are successfully applied to the analysis of the drug in its tablets and validated according to ICH guidelines. In addition, their greenness was assessed using three different tools indicating their least hazardous effect on the environment.
Proton-Pump Inhibitor Use and the Risk of Community-Associated Clostridium difficile Infection
Authors: Inghammar Malin , Svanström Henrik , Voldstedlund Marianne , Melbye Mads , Hviid Anders , Mølbak Kåre , Pasternak Björn 
Published: December 2020

Abstract

Background
Proton-pump inhibitors (PPIs) have been reported to increase the risk of community-associated Clostridium difficile infection (CDI), but the association remains disputed.

Methods
A nationwide cohort study among adults in Denmark, 2010–2013, linking register data on C. difficile testing, filled prescriptions, and patient characteristics. All incident episodes of community-associated CDI (ie, positive culture, molecular assay, or toxin test in individuals without previous hospitalization in the prior 12 weeks and without a positive test for C. difficile in the prior 8 weeks) were identified in the Danish National Microbiological Database. Self-controlled case-series analyses were used to estimate incidence rate ratios (IRRs) for community-associated CDI, comparing periods with and without exposure to PPIs. By design, models took fixed confounders such as chronic disease, genetics, and socioeconomic status into account; further, time-varying confounders, including hospital stay and antibiotic and corticosteroid use were adjusted for.

Results
3583 episodes of community-associated CDI were identified, of which 964 occurred during current use of PPIs, 324 occurred 0–6 months after treatment cessation, 123 occurred 6–12 months after treatment cessation, and 2172 occurred during time periods without use of PPIs. The adjusted IRR was 2.03 (95% confidence interval, 1.74–2.36), comparing use of PPI with nonuse. The increased risk remained elevated in later time periods: 1.54 (1.31–1.80) for 0–6 months, 1.24 (1.00–1.53) for 6–12 months after current use.

Conclusions
Use of PPIs was associated with moderately increased risk of community-associated CDI. The risk remained elevated up to 1 year after PPI treatment had ended.
Temperature management potentially affects carbon mineralization capacity and microbial community composition of a shallow aquifer
Authors: Metze Dennis , Popp Denny , Schwab Laura , Keller Nina-Sophie , da Rocha Ulisses Nunes , Richnow Hans-Hermann , Vogt Carsten 
Published: December 2020

ABSTRACTHigh-temperature aquifer thermal energy storage (HT-ATES) is a promising technique to reduce the CO2 footprint of heat supply in the frame of transitioning to renewable energies. However, HT-ATES causes temperature fluctuations in groundwater ecosystems potentially affecting important microbial-mediated ecosystem services. Hence, assessing the impact of increasing temperatures on the structure and functioning of aquifer microbiomes is crucial to evaluate potential environmental risks associated with HT-ATES. In this study, we investigated the effects of temperature variations (12–80°C) on microbial communities and their capacity to mineralize acetate in aerobically incubated sediment sampled from a pristine aquifer. Compared to natural conditions (12°C), increased acetate mineralization rates were observed at 25°C, 37°C and 45°C, whereas mineralization was decelerated at 60°C and absent at 80°C. Sequencing of 16S rRNA genes revealed that the bacterial diversity in acetate-amended and non-acetate-amended sediments decreased with rising temperatures. Distinct communities dominated by bacterial groups affiliated with meso- and thermophilic bacteria established at 45°C and 60°C, respectively, while the number of archaeal phylotypes decreased. The changes in microbial diversity observed at 45°C and 60°C indicate a potential loss of ecosystem functioning, functional redundancy and resilience, while heat storage at 80°C bears the risk of ecological collapse.
Metabolome profiles of the alphaproteobacterial methanotroph Methylocystis sp. Rockwell in response to carbon and nitrogen source
Authors: Lazic Marina , Sugden Scott , Sauvageau Dominic , Stein Lisa Y 
Published: December 2020

ABSTRACT
Methanotrophs use methane as a sole carbon source and thus play a critical role in its global consumption. Intensified interest in methanotrophs for their low-cost production of value-added products and large-scale industrialization has led to investigations of strain-to-strain variation in parameters for growth optimization and metabolic regulation. In this study, Methylocystis sp. Rockwell was grown with methane or methanol as a carbon source and ammonium or nitrate as a nitrogen source. The intracellular metabolomes and production of polyhydroxybutyrate, a bioplastic precursor, were compared among treatments to determine how the different combinations of carbon and nitrogen sources affected metabolite production. The methane–ammonium condition resulted in the highest growth, followed by the methane–nitrate, methanol–nitrate and methanol–ammonium conditions. Overall, the methane–ammonium and methane–nitrate conditions directed metabolism toward energy-conserving pathways, while methanol–ammonium and methanol–nitrate directed the metabolic response toward starvation pathways. Polyhydroxybutyrate was produced at greater abundances in methanol-grown cells, independent of the nitrogen source. Together, the results revealed how Methylocystis sp. Rockwell altered its metabolism with different combinations of carbon and nitrogen source, with implications for production of industrially relevant metabolites.
Reversal of apolipoprotein E4-dependent or chemical-induced accumulation of APP degradation products by vitamin C-induced release of heparan sulfate from glypican-1
Authors: Cheng Fang , Fransson Lars-Åke , Mani Katrin 
Published: December 2020

Abstract
The Apolipoprotein E4 (ApoE4) genotype is the most influential risk factor for sporadic Alzheimer’s disease. It appears to be associated with retarded endosome-to-autophagosome trafficking. The amyloid precursor protein (APP) and the heparan sulfate (HS)-containing proteoglycan glypican-1 (Gpc-1) are both processed in endosomes, and mutually regulated by the APP degradation products and the released HS. We have investigated APP and Gpc-1 processing in ApoE3 and ApoE4 expressing human fibroblasts, in human neural stem cells (NSC) exposed to the cholesterol transport inhibitor U18666A and in induced neurons obtained by reprogramming of ApoE fibroblasts (ApoE-iN). We have used immunofluorescence microscopy, flow cytometry, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis western blotting with antibodies recognizing the released HS, APP, amyloid β(Aβ), late endosomes (Rab7), autophagosomes (LC3) and neurons (Tuj1). We found that the capacity to release HS was not fully utilized in ApoE4 expressing fibroblasts and that HS-Aβ complexes accumulated in the nuclei. In ApoE3 fibroblasts, the β-cleaved APP C-terminal fragment (β-CTF) and Aβ were primarily present in late endosomes and autophagosomes. When HS release from Gpc-1 was enhanced by ascorbate in ApoE4/4 fibroblasts, there was efficient transfer of Aβ and HS from the nuclei to autophagosomes. In U18666A-treated NSC as well as in ApoE4/4-iN we repeatedly found accumulation of APP degradation products (β-CTF/Aβ). This was reversed by subsequent exposure to ascorbate or dehydroascorbic acid.
Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development
Authors: Gunaseelan Saravanan , Wang Ziyin , Tong Venetia Kok Jing , Ming Sylvester Wong Shu , Razar Rafhanah Banu Bte Abdul , Srimasorn Sumitra , Ong Wei-Yi , Lim Kah-Leong , Chua John Jia En 
Published: December 2020

Abstract
FEZ1-mediated axonal transport plays important roles in central nervous system development but its involvement in the peripheral nervous system is not well-characterized. FEZ1 is deleted in Jacobsen syndrome (JS), an 11q terminal deletion developmental disorder. JS patients display impaired psychomotor skills, including gross and fine motor delay, suggesting that FEZ1 deletion may be responsible for these phenotypes, given its association with the development of motor-related circuits. Supporting this hypothesis, our data show that FEZ1 is selectively expressed in the rat brain and spinal cord. Its levels progressively increase over the developmental course of human motor neurons (MN) derived from embryonic stem cells. Deletion of FEZ1 strongly impaired axon and dendrite development, and significantly delayed the transport of synaptic proteins into developing neurites. Concurring with these observations, Drosophila unc-76 mutants showed severe locomotion impairments, accompanied by a strong reduction of synaptic boutons at neuromuscular junctions. These abnormalities were ameliorated by pharmacological activation of UNC-51/ATG1, a FEZ1-activating kinase, with rapamycin and metformin. Collectively, the results highlight a role for FEZ1 in MN development and implicate its deletion as an underlying cause of motor impairments in JS patients.
Learning-based low-illumination image enhancer for underwater live crab detection
Authors: Cao Shuo , Zhao Dean , Sun Yueping , Ruan Chengzhi 
Published: December 2020

Abstract
Swift, non-destructive detection approaches should address the problem of insufficient sensitivity when attempting to obtain and perceive live crab information in low-light environments caused by the crab’s phototaxis. We propose a learning-based low-illumination image enhancer (LigED) for effective enhanced lighting and elimination of darkness in images. The camera response function was combined with the reflectance ground-truth mechanism of image decomposition. Self-attention units were then introduced in the reflectance restoration network to adjust the illumination to avoid visual defects, thus jointly strengthening the adaptability of dark-light enhancement and ability to perceive crab information. Convolutional neural network (CNN)-based detection methods can further enhance the algorithm’s robustness to light and adaptability to different environments, which motivated the development of a scalable lightweight live crab detector (EfficientNet-Det0) utilizing the two-stage compound scaling CNN approach. The lightness order error and natural image quality evaluator based on the proposed methods were 251.26 and 11.60, respectively. The quality of average precision detection increased by 13.84–95.40%. The fastest detection speed of a single image was 91.74/28.41 f·s−1 using a common GPU/CPU, requiring only 15.1 MB of storage, which advocates for the utilization of LigED and EfficientNet-Det0 for the efficient detection of underwater live crabs.
Gendering Heterodoxy: The Corporeal Politics ofXiejiaoin Chinese Christianity
Authors: Yuan Xiaobo 
Published: December 2020

AbstractIn contemporary Christian communities in mainland China, heterodox religious organizations—most often referred to as xiejiao (“evil cults”)—have been seen as perennial threats to the proper functioning of the church. State and church authorities warn against the popularization of millenarian sects like Eastern Lightning/Church of Almighty God, and ordinary churchgoers are frequently exhorted to police each other (and themselves) against heterodox thoughts and actions. This paper explores the constitution of xiejiao as an intimate threat within Chinese Protestant communities. In particular, I attend to how rural women are represented as particularly “at risk” of propagating and receiving “heterodox” religious instruction. Drawing on ethnographic research and discursive analysis, I argue that the gendered and classed dimensions of anti-cult discourse suggest a broader corporeal politics of religious identity in which spiritual endangerment and responsibility are unevenly distributed among social bodies.
Therapeutic benefit of lymphadenectomy for older patients with urothelial carcinoma of the upper urinary tract: a propensity score matching study
Authors: Ishiyama Yudai , Kondo Tsunenori , Kubota Satoshi , Shimada Katsunori , Yoshida Kazuhiko , Takagi Toshio , Iizuka Junpei , Tanabe Kazunari 
Published: December 2020

Abstract

Objectives
Regional lymphadenectomy for urothelial carcinoma of the upper urinary tract is sometimes avoided in older patients to reduce surgical burden. We aimed to evaluate the therapeutic impact of lymphadenectomy in older patients undergoing curative therapy for upper urinary tract urothelial carcinoma.

Methods
The patients with urothelial carcinoma of the upper urinary tract older than 75 years at the time of surgery and without lymph node or distant metastasis who underwent curative therapy at two tertiary hospitals between 1994 and 2019 were retrospectively analyzed. Complete-lymphadenectomy was performed as per our protocol. Cancer-specific survival, overall survival and metastasis-free survival after surgery were evaluated between complete-lymphadenectomy and no/incomplete-lymphadenectomy groups before and after 1:1 propensity score matching.

Results
The original cohort included 150 patients (median age, 80.71 years), and complete-lymphadenectomy was performed in 42 (28.00%) patients. Patients in complete-lymphadenectomy group were younger and less likely to be aged &gt;80 years (both, P &lt; 0.0001). After matching, 30 patients were allocated to each group and the ages were comparable (78.58 vs. 77.48 years, P = 0.1738). High-grade perioperative complication rates did not differ between groups both before and after matching. Cancer-specific survival, overall survival and metastasis-free survival were significantly longer in the complete-lymphadenectomy group both before and after matching (all, P &lt; 0.05).

Conclusions
This study suggests that complete-lymphadenectomy may provide therapeutic benefits for older patients. The decision to perform complete-lymphadenectomy must be based on the patient’s physical condition, rather than his/her chronological age.
HATS-34b and HATS-46b: re-characterization using TESS and Gaia
Authors: Louden Emma M , Hartman Joel D 
Published: December 2020

ABSTRACT
We present a revised characterization of the previously discovered transiting planet systems HATS-34 and HATS-46. We make use of the newly available space-based light curves from the NASA TESS mission and high-precision parallax and absolute photometry measurements from the ESA Gaia mission to determine the mass and radius of the planets and host stars with dramatically increased precision and accuracy compared to published values, with the uncertainties in some parameters reduced by as much as a factor of 7. Using an isochrone-based fit, for HATS-34 we measure a revised host star mass and radius of $0.952_{-0.020}^{+0.040}$ $\, \mathrm{M}_\odot$ and 0.9381 ± 0.0080 $\, \mathrm{R}_\odot$, respectively, and a revised mass and radius for the transiting planet of 0.951 ± 0.050 MJ and 1.282 ± 0.064 RJ, respectively. Similarly, for HATS-46 we measure a revised mass and radius for the host star of 0.869 ± 0.023 $\, \mathrm{M}_\odot$ and 0.894 ± 0.010 $\, \mathrm{R}_\odot$, respectively, and a revised mass and radius for the planet of 0.158 ± 0.042 MJ and 0.951 ± 0.029 RJ, respectively. The uncertainties that we determine on the stellar and planetary masses and radii are also substantially lower than re-determinations that incorporate the Gaia results without performing a full re-analysis of the light curves and other observational data. We argue that, in light of Gaia and TESS, a full re-analysis of previously discovered transiting planets is warranted.
MazF toxin causes alterations in Staphylococcus aureus transcriptome, translatome and proteome that underlie bacterial dormancy
Authors: Bezrukov Fedor , Prados Julien , Renzoni Adriana , Panasenko Olesya O 
Published: December 2020

Abstract
Antibiotic resistance is a serious problem which may be caused by bacterial dormancy. It has been suggested that bacterial toxin–antitoxin systems induce dormancy. We analyzed the genome-wide role of Staphylococcus aureus endoribonuclease toxin MazF using RNA-Seq, Ribo-Seq and quantitative proteomics. We characterized changes in transcriptome, translatome and proteome caused by MazF, and proposed that MazF decreases translation directly by cleaving mRNAs, and indirectly, by decreasing translation factors and by promoting ribosome hibernation. Important pathways affected during the early stage of MazF induction were identified: MazF increases cell wall thickness and decreases cell division; MazF activates SsrA-system which rescues stalled ribosomes, appearing as a result of MazF mRNA cleavage. These pathways may be promising targets for new antibacterial drugs that prevent bacteria dormancy. Finally, we described the overall impact of MazF on S. aureus cell physiology, and propose one of the mechanisms by which MazF might regulate cellular changes leading to dormancy.
OCCURRENCE AND DOSE FROM INTAKE OF RADON IN DRINKING GROUNDWATER IN OGUN STATE, NIGERIA
Authors: Fatoki Olusola Odunayo , Ademola Janet Ayobami 
Published: December 2020

AbstractRadon-222 concentrations in groundwater from 10 local government areas (LGAs) of Ogun State were measured using AlphaGUARD radon detector and AquaKIT. The mean activity concentration of radon ranged from 1.23 ± 0.21 to 12.68 ± 18.11 Bq.l−1 corresponding to geometric means (GMs) of 1.22– 6.39 Bq.l−1. The radon concentrations of all the samples were below the World Health Organization and European Commission guidance level of 100 Bq.l−1, with 17% higher than 11.1 Bq.l−1, recommended by the United States Environmental Protection Agency. Mean annual effective dose due to ingestion ranged from 0.020 ± 0.004 to 0.254 ± 0.353 mSv.y−1 (adults), 0.041 ± 0.007 to 0.509 ± 0.705 mSv.y−1 (children) and 0.024 ± 0.004 to 0.297 ± 0.411 mSv.y−1 (infants). That of inhalation varied from 0.303 ± 0.053 to 3.108 ± 4.440 μSv.y−1. The mean annual effective doses of some of the LGAs were higher than the International Commission for Radiological Protection recommended reference dose level of committed effective dose of 0.1 mSv from 1-year consumption of drinking water.
Unusual Clinical Manifestations and Outcome of Multisystem Inflammatory Syndrome in Children (MIS-C) in a Tertiary Care Hospital of North India
Authors: Gupta DCH Shobhna , Chopra MD Nidhi , Singh MD Amitabh , Gera Rani , Chellani MD Harish , Pandey PhD Ravindra , Arora MD Balvinder Singh 
Published: December 2020

AbstractTill date, there is paucity of published literature on clinical manifestations of Coronavirus disease 2019 (COVID-19) in children from low-middle-income countries (LMIC). Most of the reports are from Europe, USA or China. Our study aimed to capture data on varied and unusual clinical presentation and management of MIS-C (Multisystem Inflammatory Syndrome in Children) with COVID-19 and compare the MIS-C and non-MIS-C children. This was a single-centre cohort study of 41 COVID positive children 0–12 years age hospitalized between 1 April 2020 and 31 July 2020. Data were entered into standardized WHO Case Report Form and analysed using strata 15.0 statistical software. Twenty out of 41 children fulfilled the criteria of MIS-C. Male-to-female ratio in the cohort was 1.73:1. In MIS-C cases, predominant clinical manifestation was fever (100%), neurological manifestations (80%), lower respiratory tract infection (50%), rash (35%) and acute gastroenteritis (25%). They were categorized into Acute Encephalitis-like illness in 35%, Kawasaki-like disease, Toxic Shock-like syndrome and Comorbidity with systemic complications in 20% each. Ninety percent of MIS-C cases required oxygen supplementation with odds ratio (OR) 18 (3.22–100.48), whereas 65% required mechanical ventilation with OR 37.14 (4.08–338.10). Most of them had raised inflammatory markers and hepatic enzymes derangement. Steroids, Intravenous immunoglobulin and supportive therapy were mainstay of management for MIS-C group. Most MIS-C group children had multisystem involvement with predominant neurological manifestations at time of presentation. Delay in diagnosis and referral may have adversely affected the prognosis and outcome.
Anemia and insomnia: a cross-sectional study and meta-analysis
Authors: Neumann Samantha N. , Li Jun-Juan , Yuan Xiao-Dong , Chen Shuo-Hua , Ma Chao-Ran , Murray-Kolb Laura E. , Shen Yun , Wu Shou-Ling , Gao Xiang 
Published: December 2020

Abstract

Background
Several recent genome-wide association studies suggested insomnia and anemia may share some common genetic components. We thus examined whether adults with anemia had higher odds of having insomnia relative to those without anemia in a cross-sectional study and a meta-analysis.

Methods
Included in this cross-sectional study were 12,614 Chinese adults who participated in an ongoing cohort, the Kailuan Study. Anemia was defined as hemoglobin levels below 12.0 g/dL in women and 13.0 g/dL in men. Insomnia was assessed using the Chinese version of the Athens Insomnia Scale (AIS). A total AIS score ≥6 was considered insomnia. The association between anemia and insomnia was assessed using a logistic regression model, adjusting for potential confounders such as age, sex, chronic disease status, and plasma C-reactive protein concentrations. A meta-analysis was conducted using the fixed effects model to pool results from our study and three previously published cross-sectional studies on this topic in adult populations.

Results
Individuals with anemia had greater odds of having insomnia (adjusted odds ratio [OR]: 1.32; 95% confidence interval [CI]: 1.03–1.70) compared with individuals without anemia. A significant association persisted after we excluded individuals with chronic inflammation, as suggested by C-reactive protein levels >1 mg/L (adjusted OR: 1.68; 95% CI: 1.22–2.32). The meta-analysis results, including 22,134 participants, also identified a positive association between anemia and insomnia (pooled OR: 1.39; 95% CI: 1.22–1.57).

Conclusions
The presence of anemia was significantly associated with a higher likelihood of having insomnia in adults. Due to the nature of the cross-sectional study design, results should be interpreted with caution.