bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation of Pathogenic and Non-pathogenic Microbial Stains from Different Types of Sea Fish Samples and their Quality Assessment with Antibiogram Properties
Authors: Shahriar Asif , Akter Taheera , Kobra Anina Tul , Emran Talha Bin , Mallick Jewel , Dutta Mycal 
Published: November 2019

Aims: The contamination of sea fish and seafood by bacteria of fecal origin along with various pathogenic drug-resistant microorganisms are widely documented as the cause of several human diseases. Our present studies aim to determine the comparative microbiological quality among raw, cooked and cooked-frozen fish along with antimicrobial profiling of those isolated pathogenic and non-pathogenic microbes with potential drug modelling and increasing the awareness of taking unnecessary antibiotics. The objective of the present study was to investigate the status of fish in cold condition after cooking.
Methodology: We collected a total of four sea fish species (Rupchada, Koral, Tuna, and Poma) from the local shops in Dhaka city. Raw, cooked, and frozen fish samples were analyzed for the existence of pathogenic bacteria through conventional cultural techniques and the confirmatory biochemical identification procedures. Kirby-Bauer disk diffusion method was performed to demonstrate the antibiotic susceptibility pattern of isolated microorganisms.
Results: Total viable bacteria were present in all four fish samples in raw, cooked, and frozen condition up to 8.5 log CFU/ml. Most of the raw fish samples were found to harbour a vast population of microorganisms up to 7 log CFU/ml, including fecal coliforms. Several specific bacterial species like Escherichia coli, Klebsiella spp., Salmonella spp., Shigella spp., Staphylococcus spp., Pseudomonas spp. and Vibrio spp. were noticed to be present in raw samples. The microbial loads were reduced in cooked samples, and the status was static in frozen samples. The study of antibiogram showed several pathogenic isolates to be drug-resistant against second-line drugs.
Conclusion: Thus, the incidence of fecal coliforms in raw fish may be considered as a severe threat to public health upon consumption of such fishes. Such a prevalence of pathogens in the studied fish samples, including antibiotic-resistant strains of pathogenic microorganisms, can lead to severe public health risk and are the significant findings of this study.
Primaries Oblateness Effects on the Collinear Libration Points in the Restricted-three Body Problem
Authors: Ismail M. N. , Ibrahim A. H. , Mohamadin G. H. F. , Okasha W. A. 
Published: November 2019

In this work, the canonical Hamiltonian form of the restricted three- body problem including the effects of primaries oblateness is presented. Moreover, the collinear libration points are obtained. In addition to this, the relation between position of libration points and variation in (mass ration , oblateness coefficients A1 and A2) is studied. The results obtained are a good agreement with Perdios [1] & Singh [2]. The Poincare surface section PSS is used to illustrate the stability of motion around each of the collinear libration points. A numerical application on the real system Earth-Moon is presented.
Testing the Fairness of a Coin by Akaike's Information Criterion
Authors: Takezawa Kunio 
Published: November 2019

In this paper, AIC (Akaike's Information Criterion) is used to judge whether a coin is biased or not using the sequence of heads and tails produced by tossing the coin several times. It is well known that AIC·(−0:5) is an efficient estimator of the expected log-likelihood when the true distribution is contained in a specified parametric model. In the coin tossing problem, however, AIC·(−0:5) works as an efficient estimator even if the true distribution is not contained in a specied parametric model. Moreover, the judgement of fairness of coin using AIC is equivalent to a statistical test using the Bernoulli distribution with a signicance level ranging from 11% to 18%. This indicates that the judgement of the fairness of coin based on AIC leads to a higher probability of type I errors than that given by a statistical test with a signicance level of 5%. These findings show that we judge the fairness of a coin based on AIC when we do not have any prior knowledge about its fairness and we want to judge it from the standpoint of prediction. In contrast, a statistical test with a significance level of 5% is adopted when we have prior knowledge that the coin is probably unbiased. Moreover, a statistical test with a 5% significance level allows us to conclude that the coin is biased if we obtain sufficient evidence that permits us to disbelieve the prior knowledge.
Relationship of Calories Intake and Physical Activity in Adolescents in Colima, Mexico: A Cross-sectional Study
Authors: Castro-Cornejo María de los Ángeles , Arteaga-Herrera Xiomara , Padilla-Raygoza Nicolás 
Published: November 2019

Aims: To identify the relationship between calories intake, physical activity and body mass index in adolescents in Colima Mexico.
Study Design: A cross-sectional study analytic, observational.
Place and Duration of the Study: Colima and Tecoman, Mexico.
Methodology: It was designed in adolescents, of both genders, with a BMI in 85th percentile or greater, where the average daily calorie intake was quantified with the SNUT survey and the level of physical activity with the International Physical Activity Questionnaire. Anthropometry to obtain the BMI. The stage of change of the trans-theoretical model was identified. Statistical analysis was Pearson's r between calories and BMI and between physical activity and BMI, systolic and diastolic blood pressure with BMI, and the stages of change.
Results and Discussion: Poor correlation (r = 0.13) but significant linear relationship between calorie intake with BMI (t = 2.01 P = .05) and moderate correlation (r = - 0.41) and significant linear relationship (t =- 6.76, P = .0001) between activities physical and BMI. Between systolic and diastolic blood pressure and BMI, r= 0.28 and 0.34, respectively, with a strong linear trend in both (P = .0001) Of the adolescents, 39.32% were in the action stage (they plan to take actions to improve their overweight / obesity status) and 34% in the pre-contemplation phase (where the adolescent does not plan to do anything about his overweight / obesity).
Conclusion: There is a relationship between daily calorie intake and BMI and a strong negative relationship between physical activity and BMI, and a third of adolescents do not see overweight / obesity as a problem and have not thought of doing anything about modifying their lifestyle to improve their BMI.
Physico-chemical Alterations and Hydrocarbon Characteristics of Kom-Kom Oil Spill Soils
Authors: Onyejekwe I. M. , Osuji L. C. , Nwaichi E. O. 
Published: November 2019

Aims: The aim of this study is to find the alterations that occurred in the physico-chemical properties and the hydrocarbon content on the crude oil impacted soil in Kom-Kom, Oyigbo, Rivers State Niger Delta, Nigeria.
Study Design: The objectives included to evaluate the physico-chemical parameters of the spilled soil, determine the hydrocarbon content and that of some selected heavy metals. This will help create a baseline data on the environmental status of the area.
Place and Duration of Study: This study was carried out after an oil spill occurred in Feburary, 2018 at Kom-Kom, Oyigbo, Rivers State, Nigeria.
Methodology: The soil samples were obtained randomly at 30cm depth using soil auger from three plots: PA and PB being the plots around the oil spill impacted area and PC being the control area which is about 200m away. Laboratory analyses were carried out on the Physicochemical Parameters (pH, Electric Conductivity, Potassium (K), Phosphate (P), Nitrate (N)); Organics (Total Hydrocarbon Content (THC), Total Petroleum Hydrocarbon (TPH), Polycyclic Aromatic Hydrocarbon (PAH) and Total Organic Carbon (TOC)) and Heavy Metals (Iron (PB), Zinc (Zn), Lead (Pb), Chromium (Cr), Vanadium (V)). The data were analysed using descriptive statistics and One-Way ANOVA.
Results: pH, K and P values were all significantly different from their respective control values (p ≤0.05). All organic parameters were also significantly different from the control values (p ≤0.05). For heavy metals, only Cr and V values were significantly different in all study sites (p ≤0.05). This study shows that crude oil spill alters the physicochemical attributes of the soil and could significantly affect soil fertility as the people of Kom-Kom are mostly farmers and traders.
Conclusion: With these levels of alteration, this study will serve as a resourceful data source for soil studies in Kom Kom. In order to achieve the third sustainable development goal (SDG) which is to have good health and well-being of people, we recommend immediate and proper clean up using bioremediation approaches as a cheap, eco-friendly and an environmentally sustainable process.
A Comparative Analysis on Maritime Disaster Management between Korea and Sweden
Authors: Namkung Lock 
Published: November 2019

The purpose of this study is to illustrate the importance of crisis management planning and effective governance by comparing and analysing cases related to crisis management in disasters. Firstly, this study analyses the sinking of the MV Sewol of Korea, one of the worst ship accidents in the world, and criticise the crisis management plan. It also applies relevant methodologies to identify how to manage incidents and how to perform them for better management. Secondly, this analyses the sinking accident and crisis management plan of MS Estonia in Sweden which is one of the worst ship accident in the world. As a result, MS Estonia disaster triggered improvements in safety policies in Sweden.
This study argues that Korean government can learn important lessons from Sweden to avoid the "vicious cycle" that exists in South Korea's policy decisions related to safety issues. Compared with the case in Sweden, this study suggests that further measures are needed to end the vicious circle of low safety standards and low confidence in Korea. Compared to the case in Sweden, a few policy recommendations are suggested in this study.
Retracted: Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma
Authors: Gao Jun , Dai Chao , Yu Xin , Yin Xiang‐Bao , Zhou Fan 
Published: November 2019

The current study aimed to explore the role of the circular RNA circ‐TCF4.85 and its downstream target microRNA‐486‐5p (miR‐486‐5p) in hepatocellular carcinoma (HCC) development. Circ‐TCF4.85 was detected to be highly expressed in HCC tissues. Next, we found that silencing of circ‐TCF4.85 repressed HCC cell proliferation, invasion, and migration, while enhancing apoptosis. In addition, biotin‐coupled probe pull‐down and miRNA capture assays, as well as fluorescence in situ hybridization, confirmed that circ‐TCF4.85 could bind to miR‐486‐5p. In rescue experiments, miR‐486‐5p had the potential to eliminate the tumor‐suppressive effects of circ‐TCF4.85 knockdown in HCC. Moreover, miR‐486‐5p was shown to target ABCF2 gene, which was positively regulated by circ‐TCF4.85. Finally, nude mice subcutaneously injected with si‐circ‐TCF4.85‐transfected HCC cells presented with inhibited xenograft tumor formation in vivo. Taken together, our results reveal that silencing of circ‐TCF4.85 suppresses HCC progression via miR‐486‐5p‐targeted inhibition of ABCF2.
Spondyloarthritis and Outcomes in Pregnancy and Labor: A Nationwide Register‐Based Cohort Study
Authors: Mørk Sofie , Voss Anne , Möller Sören , Bliddal Mette 
Published: November 2019

ObjectiveTo describe the prevalence of spondyloarthritis (SpA) and subtypes diagnosed prior to delivery in a nationwide population of pregnant women, and to estimate how SpA was associated with adverse pregnancy‐related outcomes.MethodsUsing the Danish Medical Birth Register, we identified 1,199,610 singleton pregnancies (1997–2016). Information on SpA and related subtypes (ankylosing spondylitis, psoriatic arthritis, reactive arthritis, arthritis associated with inflammatory bowel diseases, and undifferentiated SpA) was derived from the Danish National Patient Registry. Odds ratios were calculated using logistic regression models. The analyses were performed with overall SpA as exposure and stratified by subtype.ResultsThe overall prevalence of SpA diagnosed prior to delivery was 0.31%, increasing from 0.1% in 1997 to 0.6% in 2016. Comparing women without SpA to women with SpA, the adjusted odds ratios were increased for moderately preterm birth (ORadj1.56 [95% confidence interval (95% CI) 1.33–1.83]), very preterm birth (ORadj1.47 [95% CI 1.04–2.08]), elective cesarean section (ORadj1.44 [95% CI 1.26–1.64]), emergency cesarean section (ORadj1.17 [95% CI 1.04–1.33]), and use of epidural (ORadj1.11 [95% CI 1.02–1.20]). The odds ratios for small for gestational age birth and preeclampsia were not increased for women with SpA compared to controls. Results were comparable for the subtypes of SpA.ConclusionPregnancies in women with SpA were more often complicated by adverse pregnancy outcomes than pregnancies in women without SpA. Clinicians should be aware of this when advising women with SpA in their childbearing years. Future research should focus on investigating causal relations and possible interventions aimed at preventing these outcomes.
Treat‐to‐Target Approach in Rheumatoid Arthritis: A Quality Improvement Trial
Authors: Desai Sonali P. , Leatherwood Cianna , Forman Malka , Ko Eunji , Stevens Emma , Iversen Maura , Xu Chang , Lu Bing , Solomon Daniel H. 
Published: November 2019

ObjectiveUsing a quality improvement approach, our objective was to integrate a treat‐to‐target approach for rheumatoid arthritis (RA) through routine electronic collection of patient‐reported disease activity scores and a multidisciplinary learning collaborative for rheumatologists.MethodsRA patients completed a patient‐reported outcome measure, the Routine Assessment of Patient Index Data 3 (RAPID3), at check‐in. Nine rheumatologists and their patients were allocated to a learning collaborative intervention group focused on a treat‐to‐target approach and 13 were allocated to a control group. The primary outcome was documentation of a treat‐to‐target implementation score: disease activity score, disease activity score used in the medication change decision, the presence of a treatment target, and an indication of shared decision‐making. A primary analysis of patient visits with medication changes was conducted using an interrupted time‐series analysis.ResultsWe studied 554 individual rheumatology patients with 709 patient visits. Treat‐to‐target implementation scores among intervention rheumatologists (mean ± SD 44.6% ± 1.63%) were 12.4% higher than in the control group (mean ± SD 32.2% ± 1.50%; P < 0.0001). We observed differences in treat‐to‐target implementation score components, comparing intervention group to control group rheumatologists: disease activity score present, 77.2% versus 68.0% (P = 0.02); disease activity score used in the medication change decision, 45.2% versus 30.0% (P < 0.01); treatment target, 9.0% versus 0.4% (P < 0.01); and shared decision‐making, 46.9% versus 30.0% (P < 0.01). Secondary analysis of patient visits with high RAPID3 scores found that medication changes were 54% less likely in the intervention versus control group (odds ratio 0.46 [95% confidence interval 0.27–0.79], P = 0.005).ConclusionThis nonrandomized, interrupted time‐series trial demonstrated a modest but significant impact of a learning collaborative intervention on rheumatologist documentation of a treat‐to‐target approach in RA.
DNA‐Mediated Assembly of Multispecific Antibodies for T Cell Engaging and Tumor Killing
Authors: Pan Liqiang , Cao Chan , Run Changqing , Zhou Liujuan , Chou James J. 
Published: November 2019

AbstractTargeting T‐cells against cancer cells is a direct means of treating cancer, and has already shown great responses in clinical treatment of B‐cell malignancies. A simple way to redirect T‐cells to cancer cells is by using multispecific antibody (MsAb) that contains different arms for specifically “grabbing” the T‐cells and cancer cells; as such, the T‐cells are activated upon target engagement and the killing begins. Here, a nucleic acid mediated protein–protein assembly (NAPPA) approach is implemented to construct a MsAb for T‐cell engaging and tumor killing. Anti ‐CD19 and ‐CD3 single‐chain variable fragments (scFvs) are conjugated to different l‐DNAs with sequences that form the Holliday junction, thus allowing spontaneous assembly of homogeneous protein–DNA oligomers containing two anti‐CD19 and one anti‐CD3 scFvs. The new MsAb shows strong efficacy in inducing Raji tumor cell cytotoxicity in the presence of T‐cells with EC50 ≈ 0.2 × 10−9 m; it also suppresses tumor growth in a Raji xenograft mouse model. The data indicates that MsAbs assembled from protein–DNA conjugates are effective macromolecules for directing T‐cells for tumor killing. The modular nature of the NAPPA platform allows rapid generation of complex MsAbs from simple antibody fragments, while offering a general solution for preparing antibodies with high‐order specificity.
Can the global end‐diastolic volume index guide fluid management in septic patients? A multicenter randomized controlled trial
Authors: Morisawa Kenichiro , Fujitani Shigeki , Homma Yosuke , Shigemitsu Kazuaki , Saito Nobuyuki , Hayakawa Katsura , Yasuda Hideto , Hifumi Toru , Rinka Hiroshi , Mayumi Toshihiko , Fujiwara Shinsuke , Murao Yoshinori , Taira Yasuhiko 
Published: November 2019

AimAn index that accurately measures intravascular volume is paramount for the optimal resuscitation of sepsis. Selecting an adequate indicator to substitute for central venous pressure (CVP) has remained an issue. The objective of our study was to compare the usefulness of standard early goal‐directed therapy (EGDT) with CVP (EGDT‐CVP) and modified EGDT with global end‐diastolic volume index (GEDI; EGDT‐GEDI) for sepsis.MethodsThis was a multicenter prospective randomized controlled study. All patients with sepsis who were expected to require mechanical ventilator support for a minimum of 48 h were included. The patients were classified into an EGDT‐CVP group and an EGDT‐GEDI group. All participants underwent the extubation protocol. The primary outcome was the ventilator‐free days over a 28‐day period.ResultsThe ventilator‐free days was not significantly different between the two groups (P = 0.59). However, the EGDT‐GEDI group showed a trend of shorter ventilator support duration (5.1 days [2.0–8.7 days] versus 3.9 days [2.4–5.7 days], P = 0.27) and length of stay in the intensive care unit (7.2 days [3.8–10.7 days] versus 5.1 days [3.7–8.8 days], P = 0.05) and a smaller 3‐day infusion balance than the EGDT‐CVP group (4,405 mL [1,092–8,163 mL] versus 3,046 mL [830–6,806 mL], P = 0.34), but the differences were not statistically significant.ConclusionAlthough there was no significant efficacy, EGDT guided by GEDI showed a trend of shorter length of stay in the intensive care unit and lower 3‐day infusion balance than the EGDT‐CVP group in sepsis. The GEDI monitoring did not appear to improve the ventilator‐free days over a 28‐day period.
The Redox Coupling Effect in a Photocatalytic RuII‐PdII Cage with TTF Guest as Electron Relay Mediator for Visible‐Light Hydrogen‐Evolving Promotion
Authors: Wu Kai , Li Kang , Chen Sha , Hou Ya‐Jun , Lu Yu‐Lin , Wang Jing‐Si , Wei Mei‐Juan , Pan Mei , Su Cheng‐Yong 
Published: November 2019

AbstractA nanocage coupling effect from a redox RuII‐PdII metal–organic cage (MOC‐16) is demonstrated for efficient photochemical H2 production by virtue of redox–guest modulation of the photo‐induced electron transfer (PET) process. Through coupling with photoredox cycle of MOC‐16, tetrathiafulvalene (TTF) guests act as electron relay mediator to improve the overall electron transfer efficiency in the host–guest system in a long‐time scale, leading to significant promotion of visible‐light driven H2 evolution. By contrast, the presence of larger TTF‐derivatives in bulk solution without host–guest interactions results in interference with PET process of MOC‐16, leading to inefficient H2 evolution. Such interaction provides an example to understand the interplay between the redox‐active nanocage and guest for optimization of redox events and photocatalytic activities in a confined chemical nanoenvironment.
Synthesis, characterization, biological screening and molecular docking of Zn(II) and Cu(II) complexes of 3,5‐dichlorosalicylaldehyde‐N4‐cyclohexylthiosemicarbazone
Authors: Mathews Nimya Ann , Begum P.M. Sabura , Kurup M.R. Prathapachandra 
Published: November 2019

A 3,5‐dichlorosalicylaldehyde‐N4‐cyclohexylthiosemicarbazone (C14H16Cl2N3OS) and its complexes [Zn(dsct)(phen)]·DMF (1), [Zn(dsct)(bipy)]·DMF (2), [Cu(dsct)(bipy)]·DMF (3) (phen = 1,10‐phenathroline, bipy = 2,2’bipyridine) were synthesized and characterized by CHN analysis, FT‐IR, UV–vis and NMR spectra. The molecular structure of the thiosemicarbazone (H2dsct) and its complexes have been resolved using single crystal XRD studies. In the complexes, thiosemicarbazone exist in the thioiminolate form and acts as dideprotonated tridentate ligand coordinating through phenolic oxygen, thioiminolate sulfur and azomethine nitrogen. The antibacterial activity of the prepared compounds were screened against Escherichia coli, Salmonella typhi, Enterobacter aerogenes, Shigella dysentriae, Bacillus cereus, Staphylococcus aureus. All the complexes showed activity against bacterial strains E.coli and Salmonella typhi. The thiosemicarbazone showed activity against three bacterial strains such as E. coli, Enterobacter aerogenes and Shigella dysentriae. Complex 2 showed very good antibacterial activity as compared to standard drug (Ampicillin) against the bacterial strain, Salmonella typhi. Finally, the thiosemicarbazone and its complexes have been used to accomplish molecular docking studies against an Epidermal Growth Factor Receptor (EGFR) and breast cancer mutant 3hb5‐oxidoreductase to determine the most preferred mode of interaction. The results confirm that the complex [Cu (dsct)(bipy)]·DMF(3) showed the highest docking score as compared to other complexes under study. The [Cu(dsct)(bipy)]·DMF(3) complex was evaluated for their anticancer activities against breast cancer cell line (MCF‐7) and normal L929 (Mouse Fibroblast) cell line. It was found that the compound showed an LC50 of 6.25 μg/mL against breast cancer cell line (MCF‐7).
Effect of crosslinking agent on the properties of low‐temperature curing PES/PTFE anticorrosive coating
Authors: Zhang Bo , Wang Hongyue , Zhu Shiyang , Yao Hongyan , Di Yizeng , Cao Guangzhao , Song Ying , Tan Haiwei , Yu Huaxuan , Guan Shaowei 
Published: November 2019

ABSTRACTTo reduce energy consumption and broaden the scope of application of polyethersulfone/polytetrafluoroethylene (PES/PTFE) coatings, three hybrids have been designed by incorporating PES/PTFE coatings with different contents (0, 10, and 20 wt %) of crosslinking agent consisting of epoxy resin and appropriate curing agent (ER‐CA) in the application as low‐temperature curing PES/PTFE coatings and named as EP‐0, EP‐1, and EP‐2, respectively. The addition of ER‐CA gives rise to satisfactory distribution of PTFE, making PTFE play its roles better and improves barrier properties of the coatings by secondary leveling and its own crosslinking structure, which greatly enhances anticorrosion properties. Compared with EP‐0 and EP‐2, EP‐1 exhibits lower water permeability. Even after long‐term exposure to corrosive environments, EP‐1 still possesses high anticorrosion properties. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48740.