bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nutritional analyses of Black tiger prawn (Penaeus monodon) and Nile tilapia (Oreochromis niloticus) from Sri Lanka
Authors: Premarathna Amal Dharmapriya , Kumara A.M.C.P , Jayasooriya A.P , Satharasinghe Dilan A , Pathirana E. 
Published: January 2020

Abstract
Objective Fish and crustacean are important sources of animal proteins and other essential nutrients required in the human diet. They are widely accepted for their high palatability, high protein content and tender flesh. In this study, proximate analyses were done for flesh of brackish water shrimp species Penaeus monodon and fresh water fish species Oreochromis niloticus .Results Percentage of protein content was high values in Penaeus monodon (9.31±0.63) compared to Oreochromis niloticus (7.85±0.79). The highest lipid content was present in Penaeus monodon (0.23±0.22%) while the lowest value was shown by Oreochromis niloticus (0.07±0.16%). Comperativily, the percentage of total carbohydrate content is the highest in Brackish water shrimp than fresh water fish Nile tilapia. The study revealed high protein content and a high fat content in the meat of Penaeus monodon . Information on the nutrient composition of Penaeus monodon is needed to encourage the processing, utilization and marketing of this commonest species of Sri Lanka. It can then be inferred that the shrimps ( Penaeus monodon ) could be employed as a supplement of protein and minerals, so as to balance human nutrition. Hence the consumption of shrimps ( Penaeus monodon ) would help as a good source of nutrients.
Long-term outcomes of patients undergoing uncemented or cemented arthroplasty revision following metal-on-metal total hip arthroplasty failure: A retrospective observational study with a mean follow-up of 7 years
Authors: Chen Wenli , Shuai Mao , Lin Jinluan , Chen Baomin , Zhao Mingdong , Zhang Xinchao , Yu Weiguang , Han Guowei 
Published: January 2020

Abstract
Background A high rate of complications due to metal-on-metal (MoM) revisions has been relatively commonly documented. The purpose of this retrospective study was to compare the long-term outcomes of patients who had undergone uncemented or cemented total hip arthroplasty (THA) revision for prior primary MoM THA failure.Methods Data from 234 patients (234 hips) who underwent uncemented or cemented THA (UTHA or CTHA) for prior primary MoM THA failure during 2007 - 2018 were retrospectively analysed. Follow-up occurred 3 months, 6 months, 1 year, 2 years, and then every 1 year after conversion. The mean follow-up time was 84.15 months (range, 67 - 101 months). The primary endpoint was the modified Harris Hip Score (HHS). The secondary endpoint was the major orthopaedic complication rate.Results The HHS demonstrated statistically greater differences in the CTHA group than in the UTHA group 12 months after conversion. From the 12th month after conversion to the final follow-up, CTHA yielded better functional outcomes than UTHA. There were significant differences in the rates of re-revision, aseptic loosening, and periprosthetic fracture between the groups (10.3% for UTHA vs 2.5% for CTHA, p = 0.015; 16.3% for UTHA vs 5.9% for CTHA, p = 0.011; and 12.0% for UTHA vs 4.2% for CTHA, p = 0.045, respectively).Conclusion In the setting of revision for primary MoM THA failure, we found definite evidence of the superiority of CTHA over UTHA in terms of improving functional outcomes and decreasing the major orthopaedic complication rate.
Arthroscopic ankle fusion only has a limited advantage over the open operation if the condition is similar: a retrospective comparative study
Authors: Wang Chenggong , Xu Can , Li Mingqing , Li Hui , Wang Long , Zhong Da , Liu Hua 
Published: January 2020

Abstract
Background: A great deal of research suggests that arthroscopic ankle fusion (AAF) has advantages over open ankle fusion (OAF), but these outcomes would be imprecise because of a selection bias. The purpose of this study is to verify which is better for ankle fusion, AAF or OAF. We regrouped the OAF group into two subgroups according to whether the disease condition is similar to AAF group. The goal is to minimize the impact of disease severity, thereby reducing selection bias to some extent. Methods: We retrospectively analyzed the data of ankle fusion in our hospital between July 2015 and October 2018. 43 patients were enrolled and divided into AAF group (n=17) and OAF group (n=26). In order to eliminate selection bias, we divided OAF group into serious condition subgroup (SC subgroup) (n=15) and not serious condition subgroup (NSC subgroup) (n=11). The NSC subgroup condition is similar to AAF group. Then, we compared the differences between these groups. All patients were followed up at least one year after operation. We analyzed data, including etiology composition, surgical time, intra-op blood loss, reduction of albumin, total hospital stays, union time, fusion situation, complications, radiological examination, functional score and questionnaire survey. Then we performed statistical analyses. Results: We found that the etiological components of AAF group and OAF group were different, the etiological components of AAF group and NSC subgroup were similar. We found that AAF group has advantages over OAF group and SC subgroup in general. However, except in terms of surgical trauma, hospital stays and short-term complications occurred, the AAF group has not obvious advantages over NSC subgroup, including intra-op blood loss, fusion condition, postoperative function score and postoperative patient satisfaction; and AAF group need more surgical time than the NSC subgroup. Conclusions: The arthroscopic ankle fusion can bring a good curative effect. However, if the condition is similar, the arthroscopic ankle fusion only has a limited advantage over the open operation in beautiful incision, perioperative soft tissue protection and enhanced recovery after surgery.
Multidisciplinary Cooperation Alleviates Postoperative Pain after Elective Craniotomies: A Prospective Randomized Controlled Study of Neurosurgical Enhanced Recovery After Surgery (ERAS) program
Authors: Qu Liang , Wang Yuan , Liu Bolin , Zhang Haitao , Li Zhengmin , Niu Jiangtao , Zhao Binfang , Zhao Tianzhi , Jiang Xue , Ye Lin , Zhao Lanfu , Lv Wenhai , Zhang Yufu , Zheng Tao , Xue Yafei , Chen Lei , Chen Long , Wu Yingxi , Li Mingjuan , Ma Lin , Li Ruigang , Li Juan , Yan Jing , Wang Shasha , Zhao Hui , Sun Xude , Gao Guodong , Wang Xuelian , Qu Yan , He Shiming 
Published: January 2020

AbstractObjective: To prospectively evaluate the efficacy of neurosurgical enhanced recovery after surgery (ERAS) protocol on the management of postoperative pain after elective craniotomies. Methods: This randomised controlled trial was conducted in the neurosurgical center of Tangdu Hospital (Fourth Military Medical University, Xi’an, China). A total of 129 patients undergoing craniotomies between October 2016 and July 2017 were enrolled in a randomized clinical trial comparing ERAS protocol and conventional care. The primary outcome was the postoperative pain score assessed by a verbal numerical rating scale (NRS). Results: Patients in the ERAS group had a significant reduction in postoperative pain score on POD 1 compared to patients in the control group (mean NRS 3.12 vs. 4.44, OR 0.0968, 95% CI 0.3299 to 2.317, p = 0.010). More patients (n = 44, 68.8%) in the ERAS group experienced mild pain (NRS: 1 to 3) on POD1 compared with patients (n = 23, 35.4%) in the control group (p < 0.05). A significant reduction in pain score was observed on POD 2 and POD 3 in the ERAS group compared with that in the control group (POD2: mean NRS 2.85 vs. 4.32, OR 0.2628, 95% CI 0.5619 to 2.379, p=0.002. POD3: mean NRS 2.32 vs. 4.03, OR 0.1468, 95% CI 0.9537 to 2.458, p < 0.001, respectively). In addition, the median postoperative length of hospital stay was significantly decreased with the incorporation of ERAS protocol compared to the controls (ERAS: 4 days, control: 7 days, P<0.001). Conclusion: Implementation of the neurosurgical ERAS protocol for elective craniotomy patients have significant benefits in alleviating postoperative pain and enhancing recovery after surgery compared to the conventional care. Further evaluation of this protocol in larger, multi-center studies is warranted.
Exploring risk factors for intraoperative aneurysm rupture during clipping
Authors: Zhu Zhi , Song Ningning , Kato Yoko , Chen Xi , Jiang Weichao , Li Yukui , Xiao Fei , Fan Chaofan , Tan Guowei , Chen Sifang 
Published: January 2020

Abstract
Objective To investigate risk factors for aneurysm rupture in intracranial aneurysm clipping (IAC). Methods Patients admitted for IAC from April 2010 to December 2017 in the Fujita Health University Hospital or the First Affiliated Hospital of Xiamen University were retrospectively reviewed. Clinical parameters were recorded and analyzed using univariate and multivariate analysis. The Hunt-Hess grade was used to assess the preoperative clinical status of patients. Modified Rankin Scale was applied to evaluate the prognosis of patients 6 months after surgery. Results Univariate analysis showed that the preoperative clinical status ( p = 0.015) and the preoperative aneurysm rupture ( p = 0.005) were significantly associated with intraoperative aneurysm rupture (IAR) during clipping. Multivariate logistic regression analysis showed that the preoperative aneurysm rupture was an independent risk factor of IAR ( p < 0.001, OR = 10.518). There was no significant difference in the prognosis between patients with and without IAR ( p > 0.05). No significant differences existed on aspects of incidences and time points of rupture in the operations conducted by experienced surgeons compared with that conducted by less-experienced surgeons ( p > 0.05). Conclusion Preoperative aneurysm rupture is the independent risk factor for aneurysm rupture during IAC. Intraoperative rupture, if treated properly in time, has no influence on the prognosis of patients receiving IAC. Less-experienced surgeons can also reduce the incidence rate of IAR by strictly controlling surgical indications.
Multimodal Imaging and Genetic Characteristics of Chinese Patients with USH2A-Associated Nonsyndromic Retinitis Pigmentosa
Authors: Chen Chong , Sun Qiao , Gu Mingmin , Qian Tianwei , Luo Dawei , Liu Kun , Xu Xun , Yu Suqin 
Published: January 2020

Abstract
Background To determine the clinical characteristics and molecular genetic background responsible for USH2A mutations associated with nonsyndromic retinitis pigmentosa (RP) in five Chinese families, a retrospective cross-sectional study was performed. Data of detailed history and comprehensive ophthalmological examinations were extracted from medical charts. Genomic DNA was sequenced by whole-exome sequencing. The pathogenicity predictions were evaluated by in silico analysis. The structural modeling of the wide-type and mutant USH2A proteins was displayed based on I-Tasser software.Results The ultrawide-field fundus imaging showed a distinctive pattern of hyperautofluorescence in the parafoveal ring with macular sparing. Ten USH2A variants were detected, including seven missense mutations, two splicing mutations and one insertion mutation. Six of these variants have already been reported, and the remaining four were novel. Of the de novo mutations, the p.C931Y and p.G4489S mutations were predicted to be deleterious or probably damaging; the p.M4853V mutation was predicted to be neutral or benign; and the IVS22+3A>G mutation was a splicing mutation that could influence mRNA splicing and affect the formation of the hairpin structure of the USH2A protein.Conclusions Our data further confirm that USH2A plays a pivotal role in the maintenance of photoreceptors and expand the spectrum of USH2A mutations that are associated with nonsyndromic RP in Chinese patients.
Prevalence and risk factors for surgical site infection after colorectal surgery: a multiple-center prospective study of 3,663 consecutive patients in China
Authors: Zhang Yu , Gan Hui-Quan , Zhou Jing-Fang , Gong Ya-Jie , Li Liu-Yi , Zhang Xin-Qiang , Meng Yue , Chen Jie-Rong , Liu Wei-Jiang , Ye Long , Wang Xiao-Xiao , Zhao Yun-Hu , Hou Tie ying 
Published: January 2020

Abstract
Background: Surgical site infection (SSI) after colorectal surgery (CRS) remains a significant problem for its negative clinical outcomes. However, it is poorly understood in China . This study aims to investigate the prevalence, risk factors and microbiology of SSI after CRS. Methods: A nationwide prospective multicenter design was applied. Patients in 19 Chinese hospitals from 2015 to 2018 were prospectively monitored for SSI after CRS. Demographic data, hospital characteristics, and potential perioperative risk factors were collected and analyzed, using univariate and multivariate logistic regression models. Results: Among 3,663 study participants, 134(3.66%) episodes of SSI were identified. The prevalence rate of SSI decreased from 5.9 infections per 100 procedures in 2014 to 3.1 infections per 100 procedures in 2017 (prevalence rate ratio [PRR], 0.52; 95% CI, 0.28–0.94). The SSI rates were 1.88, 4.15, 6.27 and 11.58 per 100 operations for the National Nosocomial Infections Surveillance system (NNIS) risk index categories of 0, 1, and 2 or 3, respectively. Escherichia coli (54/134, 40.3%) and Klebsiella pneumoniae (10/134, 7.5%) were the most frequently isolated microorganisms. A high prevalence of antibiotic resistance were observed in our study, with rates of extended spectrum beta-lactamase-producing or carbapenem-resistant Escherichia coli and Klebsiella pneumonia of 50.0%(27/54) and 30.0%(3/10) respectively. Preoperative hospital stay ≥ 48h (OR=2.28, 95% CI: 1.03–5.02) and contaminated or dirty wound (OR=3.38, 95% CI: 1.88–6.06) were significantly associated with increasing risk of SSI after CRS. Conclusion: A statistically significant but modest decrease in the prevalence rate of CRS SSI over the 4-year study period was observed in this study. Noticeably, the relatively high rates of multidrug-resistant pathogens causing SSI after CRS should be alert despite of the small number of isolates identified in our survey.
Increasing lipid productivity in Chlamydomonas by engineering lipid catabolism using the CRISPR-Cas9 system
Authors: Nguyen Thu Ha Thi , Park Seunghye , Jeong Jooyeon , Shin Ye Sol , Sim Sang Jun , Jin EonSeon 
Published: January 2020

Abstract
Background Currently, most of the attention in renewable energy industry is focused on the development of alternative, sustainable energy sources. Microalgae are a promising feedstock for biofuel production in response to the energy crisis. The use of metabolic engineering to improve yields of biofuel-related lipid components in microalgae, without affecting cell growth, is now a promising approach to develop more sustainable energy sources and to make this approach more economically feasible. Results The CRISPR-Cas9 system was successfully applied to generate a target-speciﬁc knockout of the ELT gene in Chlamydomonas reinhardtii . The target gene encodes an enzyme involved in lipid catabolism, in which the knockout phenotype impacts fatty acid degradation. As a result, the knockout mutants show up to 28.52% increased total lipid accumulation in comparison with the wild-type strain. This is also accompanied by a shift in the fatty acid composition with an increase of up to 27.2% in the C18:1 proportion. These changes do not signiﬁcantly impact cell growth. Conclusion This study provides useful insights for the improvement of the oleaginous microalgae strain for biodiesel production. The acquired elt mutants showed improved lipid accumulation and productivity without compromising the growth rate.
Proteome Characteristics of Liver Tissue from Patients with Parenteral Nutrition-Associated Liver Disease
Authors: Mamtawla Gulsudum , Tian Feng , Sun Haifeng , Zhang Li , Gao Xuejin , Xue Bin , Wang Xinying 
Published: January 2020

Abstract
Background Parenteral nutrition (PN)-associated liver disease (PNALD) is a common and life-threatening complication in patients receiving PN. However, its definitive etiology is not yet clear. Therefore, performed proteomic analyses of human liver tissue to explore the same.Methods Liver tissue was derived and compared across selected patients with (n = 3) /without (n = 4) PNALD via isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-based quantitative proteomics. Bioinformatics analysis was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases to explore the mechanisms of PNALD based on differentially expressed proteins (DEPs). The essential proteins that were differentially expressed between the two groups were explored and verified by western blotting.Results A total of 112 proteins were found to be differentially expressed, of which 73 were down-regulated, and 39 were up-regulated in the PNALD group. Bioinformatics analysis showed DEPs to be associated with mitochondrial oxidative phosphorylation (mainly involved in mitochondrial respiratory chain complex I assembly), hepatic glycolipid metabolism (involved primarily in glycogen formation and gluconeogenesis), and oxidative stress (mainly involved in antioxidant change).Conclusion Overall, our results indicated that mitochondrial energy metabolism impairment, hepatic glycolipid metabolism disorder, and excessive oxidative stress injury might explain the comprehensive mechanism underlying PNALD. Moreover, we have provided multiple potential targets for further exploring the PNALD mechanism.
Dietary Momordica Cochinchinensis aril ameliorates metabolic dysfunction, nonalcoholic fatty liver, and gut microbiota in diet-induced obese mice
Authors: Lin Yu-Chun , Huang Hsiu-Chen , Lai Yu-Heng , Chen Jui-Chieh , Tien Hsiao-Hsuan , Chen Ying-Fang , Chen Yu-Hsin , Huang Cheng 
Published: January 2020

Abstract
Background Obesity and its associated conditions, such as type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease (NAFLD), are a particular worldwide health problem at present. Momordica cochinchinensis fruit is consumed widely in Southeast Asia. However, whether it has functional effects on fat-induced metabolic dysfunction and gut microbiota remains unclear. This study was conducted to determine how Momordica cochinchinensis aril (MCA) affects obesity, nonalcoholic fatty liver, insulin resistance and gut microbiota in diet-induced obese mice.Methods Wild type male mice at age of 5 weeks received four different kinds of diets: a normal diet, high-fat diet (HFD), or HFD supplemented with 1% or 3% (wt:wt) lyophilized MCA for 10 weeks. Body weight, adipose tissue and liver weight, serum biochemical parameters, glucose tolerance and liver lipids were measured. Gut microbial composition was analyzed.Results MCA protected the mice against high-fat diet (HFD)-induced body weight gain, hyperlipidemia and hyperglycemia, compared with mice that were not treated. MCA inhibited the expansion of adipose tissue and adipocyte hypertrophy. In addition, the insulin sensitivity-associated index that evaluates insulin function was also significantly restored. MCA also regulated the secretion of adipokines in HFD-induced obese mice. Moreover, hepatic fat accumulation and liver inflammation were reduced, which suggested that fatty liver was prevented by MCA. Furthermore, MCA supplementation suppressed hepatic lipid accumulation by activation of AMPK and PPAR-alpha signaling pathway in the human fatty liver HuS-E/2 cell model. Supplementation with MCA resulted in microbiota populations changed significantly.Conclusion Our data indicate that dietary MCA is involved in the prevention of HFD-induced adiposity, insulin resistance and nonalcoholic fatty liver disease and altered the microbial contents of the gut and modulated microbial dysbiosis in the host.
Assessing Clinical Embryology research: A global bibliometric analysis
Authors: Simopoulou Mara , Sfakianoudis Konstantinos , Maziotis Evangelos , Rapani Anna , Giannelou Polina , Pantou Agni , Anifandis George , Bakas Panagiotis , Vlahos Nikolaos , Pantos Konstantinos , Koutsilieris Michael 
Published: January 2020

Abstract
Background: The evaluative strength of available bibliometric tools in the field of clinical embryology has never been examined in the literature. This work aims to uniquely bring insight regarding the identity of clinical embryology research, introducing concerns when solely relying on the methodology of bibliometric analysis. Methods: An all-inclusive analysis of the most bibliometrically highlighted scientific contributions with regards to the cornerstones of clinical embryology was performed employing the Scopus, Web of Science and PubMed databases. Identified articles corresponded to the time period 1978-2018. Statistical analysis was performed employing the chi square test, and the Spearman’s Correlation coefficient. An analysis of the number of publications, respective citations and h-index, g-index, along with m-quotient is presented. The top 30 contributing authors, for each distinctive area of research are listed. An attempt at visualizing the yearly published articles, clusters and collaborations of authors, along with the geographic origin of publications is also presented. Results: Combining all searches and keywords yielded 54,522 results. In the Scopus database, employing the keyword “In Vitro Fertilization” yielded 41,292 results. The publications of the top 5 authors in each research field were analytically presented and compared to the total number of publications for each respective field. The research field of Preimplantation Genetic Diagnosis/Screening/Testing (PGD/PGT-A) was allocated the highest percentage of publications produced by the top 5 authors (11%). Regarding journal bibliometrics, based on the year 2017 metrics, there are only 29 journals according to WoS that refer to “Reproductive Biology”, ranking it 187th among 235 disciplines. Out of 29 journals, only 4 are not dedicated to human studies. The USA produced the highest number of publications (12,537).Conclusions: Results indicate an explosion of interest published in the literature regarding the field of clinical embryology. Further analysis on collaborations and the trends involved should be of added value as productivity between countries varies significantly. Data provides a critical analysis on the value of research methodology entailing bibliometric tools. This may provide researchers, In-Vitro-Fertilization professionals and prospective authors with direction and guidance during literature search, while proving useful regarding manuscript design and concurring on keywords and abstract content.