bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stromal vascular fraction cells as biologic coating of mesh for hernia repair
Authors: Guillaume O. , Pérez-Köhler B. , Schädl B. , Keibl C. , Saxenhuber N. , Heimel P. , Priglinger E. , Wolbank S. , Redl H. , Petter-Puchner A. , Fortelny R. 
Published: February 2020

Abstract
Background
The interest in non-manipulated cells originating from adipose tissue has raised tremendously in the field of tissue engineering and regenerative medicine. The resulting stromal vascular fraction (SVF) cells have been successfully used in numerous clinical applications. The aim of this experimental work is, first to combine a macroporous synthetic mesh with SVF isolated using a mechanical disruption process, and to assess the effect of those cells on the early healing phase of hernia.

Methods
Human SVF cells combined with fibrin were used to coat commercial titanized polypropylene meshes. In vitro, viability and growth of the SVF cells were assessed using live/dead staining and scanning electron microscopy. The influence of SVF cells on abdominal wall hernia healing was conducted on immunodeficient rats, with a focus on short-term vascularization and fibrogenesis.

Results
Macroporous meshes were easily coated with SVF using a fibrin gel as temporary carrier. The in vitro experiments showed that the whole process including the isolation of human SVF cells and their coating on PP meshes did not impact on the SVF cells’ viability and on their capacity to attach and to proliferate. In vivo, the SVF cells were well tolerated by the animals, and coating mesh with SVF resulted in a decrease degree of vascularity compared to control group at day 21.

Conclusions
The utilization of SVF-coated mesh influences the level of angiogenesis during the early onset of tissue healing. Further long-term animal experiments are needed to confirm that this effect correlates with a more robust mesh integration compared to non-SVF-coated mesh.
Improvements in turbulence model realizability for enhanced stability of ocean forecast and its importance for downstream components
Authors: Brüning Thorger 
Published: February 2020

AbstractThe numerical stability of ocean circulation models is of high significance in operational forecasting. A substantial improvement in numerical stability of the 3D-ocean model HBM could be achieved by the implementation of new realizability criteria in the turbulence closure scheme. Realizability criteria which were already well documented for closure functions without double diffusion were therefore extended to those using double diffusion. A purely technical validation method called ε-test which is suitable for the detection of numerical stability problems is presented, and the effect of the development in turbulence model is demonstrated under severe weather conditions during extreme storm events. Evaluation of statistics of longer simulations indicate that instabilities appeared only locally and temporary; nevertheless, a significant impact on drift products relying on the current forecasts could be demonstrated, which underlines the importance of realizability in turbulence closure schemes in comprehensive operational model systems including ocean circulation and downstream drift components.
The oldest stage of the Outer Carpathian evolution in the light of Oxfordian–Kimmeridgian exotic clast studies (southern Poland)
Authors: Kowal-Kasprzyk Justyna , Krajewski Marcin , Gedl Przemysław 
Published: February 2020

AbstractIn the Late Jurassic, the rifting process led to the disintegration of the southern margin of the North European Platform and to the opening of the Outer Carpathian flysch basin sedimentary system. The initial sediments accumulated in the northern part of the basin are related to both the destruction and resedimentation of older platform deposits. Since the sedimentary succession of this pre-flysch phase was consumed by the Miocene subduction, its only traces are represented, nowadays, by clasts preserved as exotics in the succeeding flysch deposits. Our analysis of foraminifers as well as calcareous and organic dinoflagellate cysts found in these exotics confirms the Oxfordian–early Kimmeridgian timing of the platform phase that preceded the opening of the flysch basin. The exotics are represented by three main facies types: sponge–microbial limestones, oncoid–intraclastic–Crescentiellalimestones and fine-grained, biodetrital limestones withSaccocoma. These deposits are related to mid-ramp to outer-ramp settings. The land influence was rather weak, and these sedimentary settings were dominated by pelagic/hemipelagic accumulation. The studied facies are similar to facies types widely distributed over the northern shelf area of the Western Tethys (e.g., extra-Carpathian southern Poland, Carpathian Foredeep basement, southern Germany). In turn, coeval strata known from the part of the Magura Basin and of the Penninic–Pieninic Ocean, which were situated in more southern part of the Tethys, yielded different microfacies reflecting significant differences between the sedimentary settings of the study area and its southern extensions.
Curative-intent pancreas resection for pancreatic metastases: surgical and oncological results
Authors: Chikhladze Sophia , Lederer Ann-Kathrin , Kühlbrey Christian M. , Hipp Julian , Sick Olivia , Fichtner-Feigl Stefan , Wittel Uwe A. 
Published: February 2020

Abstract
Background
Pancreatic metastasis is a rare cause for pancreas surgery and often a sign of advanced disease no chance of curative-intent treatment. However, surgery for metastasis might be a promising approach to improve patients’ survival. The aim of this study was to analyze the surgical and oncological outcome after pancreatic resection of pancreatic metastasis.

Methods
This is a retrospective cohort analysis of a prospectively-managed database of patients undergoing pancreatic resection at the University of Freiburg Pancreatic Center from 2005 to 2017.

Results
In total, 29 of 1297 (2%) patients underwent pancreatic resection due to pancreatic metastasis. 20 (69%) patients showed metastasis of renal cell carcinoma (mRCC), followed by metastasis of melanoma (n = 5, 17%), colon cancer (n = 2, 7%), ovarian cancer (n = 1, 3%) and neuroendocrine tumor of small intestine (n = 1, 3%). Two (7%) patients died perioperatively. Median follow-up was 76.4 (range 21–132) months. 5-year and overall survival rates were 82% (mRCC 89% vs. non-mRCC 67%) and 70% (mRCC 78% vs. non-mRCC 57%), respectively. Patients with mRCC had shorter disease-free survival (14 vs. 22 months) than patients with other primary tumor entities.

Conclusion
Despite malignant disease, overall survival of patients after metastasectomy for pancreatic metastasis is acceptable. Better survival appears to be associated with the primary tumor entity. Further research should focus on molecular markers to elucidate the mechanisms of pancreatic metastasis to choose the suitable therapeutic approach for the individual patient.
A Closed-Cycle Miniature Dilution Refrigerator for a Fast-Cooldown 100 mK Detector Wafer Test Cryostat
Authors: Azzoni S. , May A. J. , Chase S. T. , Coppi G. , Kenny L. C. , Melhuish S. J. , Piccirillo L. , Suzuki A. , Wenninger J. 
Published: February 2020

AbstractThe forthcoming generation of cosmic microwave background polarization observatories is developing large format detector arrays which will operate at 100 mK. Given the volume of detector wafers that will be required, fast-cooldown 100 mK test cryostats are increasingly needed. A miniature dilution refrigerator (MDR) has been developed for this purpose and is reported. The MDR is precooled by a double-stage $${^3}\mathrm{He}$$3He–$${^4}\mathrm{He}$$4He Chase Research Cryogenics sorption refrigerator. The test cryostat based on this MDR will enable fast cooldown to 100 mK to support rapid feedback testing of detector wafers fabricated for the Simons Observatory. The MDR has been designed to provide a 100 mK stage to be retrocompatible with existing CRC10 sorption coolers, reducing the base temperature from 250 mK for the new generation of detectors. Other 250 mK cryostats can be retrofitted in the same way. This configuration will meet the cryogenic requirements for single-wafer testing, providing 5–10 $$\upmu \mathrm{W}$$μW of cooling power at 100 mk for over 8 h. The system operates in a closed cycle, thereby avoiding external gas connections and cold o-rings. No moving parts are required, with the system operated entirely by heaters.
Deconvoluted plasma DSC curves on patients with psoriasis
Authors: Lőrinczy Dénes , Moezzi Mehdi , Ferencz Andrea 
Published: February 2020

AbstractPsoriasis is an inflammatory disease that changes plasma composition, and it is detectable by differential scanning calorimetry (DSC). Besides the general change in plasma, the aim of the study was to demonstrate which components are changing and how the anti-psoriatic drug treatment affects back all this. Retrospectively, blood plasma DSC data were analyzed from patients, who have different severities of symptoms and who received steroids (n = 10), or retinoids (n = 10), or biological drug treatment (n = 10). Complex curves were deconvoluted in several individual transitions (Tm1–Tm5), modeling each individual transition. In the examined psoriasis stages, the thermodynamic parameters excess heat capacity and enthalpy of the transitions in proportion corresponded to the targeted treatment and the degree of disease severity, as well as the numbers of transitions were determined from the calorimetric profiles. In conclusion, deconvoluted plasma DSC profiles showed similarities but exhibited marked differences in the thermal denaturation on different treated psoriasis stages. This examination has shown that drug therapy affects the composition of plasma proteins, which should be always considered for the evaluation of DSC results in similar studies.
Nonlinear dynamics of a vehicle with a displacement-sensitive mono-tube shock absorber
Authors: Łuczko Jan , Ferdek Urszula 
Published: February 2020

AbstractThe paper presents a new concept of absorbing car body vibrations, which consists in a modification of the construction of the classical mono-tube hydraulic shock absorber by the introduction of an additional inner cylinder with an auxiliary piston. By making an appropriate selection of the system parameters, one may obtain the damping force characteristics dependent on the excitation amplitude and frequency. In the case of driving on a good-quality road surface, the shock absorber displays the soft characteristics which are desired as far as the driving comfort is concerned. In the case of worse-quality roads or while overcoming large obstacles, the hard characteristics ensure a higher level of safety and protect the shock absorber from getting damaged. The developed nonlinear model makes it possible to effectively analyse the system responses to harmonic, impulse and random excitations. On the basis of the analysis of the impact of harmonic excitations on the driving comfort and safety indexes, one may estimate the optimal values of the shock absorber construction parameters. Impulse and random excitations are applied in order to finally verify the effectiveness of the operation of the proposed shock absorber.
Thermodynamic, Spatial and Methodological Considerations for the Manufacturing of Therapeutic Polymer Nanoparticles
Authors: Maslanka Figueroa Sara , Fleischmann Daniel , Beck Sebastian , Goepferich Achim 
Published: February 2020

Abstract
Purpose
Evaluate fundamental parameters that dictate the effectiveness of drug loading.

Methods
A model water-soluble drug lacking ionizable groups, pirfenidone (PFD), was encapsulated through nanoprecipitation in poly(ethylene glycol)-poly(lactic acid) (PEG-PLA)-poly(lactic-co-glycolic acid) (PLGA) NPs. Firstly, the thermodynamic parameters predicting drug-polymer miscibility were determined to assess the system’s suitability. Then, the encapsulation was evaluated experimentally by two different techniques, bulk and microfluidic (MF) nanoprecipitation. Additionally, the number of molecules that fit in a particle core were calculated and the loading determined experimentally for different core sizes. Lastly, the effect of co-encapsulation of α-lipoic acid (LA), a drug with complementary therapeutic effects and enhanced lipophilicity, was evaluated.

Results
The thermodynamic miscibility parameters predicted a good suitability of the selected system. MF manufacturing enhanced the encapsulation efficiency by 60–90% and achieved a 2-fold higher NP cellular uptake. Considering spatial constrictions for drug encapsulation and increasing the size of the PLGA core the number of PFD molecules per NP was raised from under 500 to up to 2000. More so, the co-encapsulation of LA increased the number of drug molecules per particle by 96%, with no interference with the release profile.

Conclusions
Thermodynamic, spatial and methodological parameters should be considered to optimize drug encapsulation.
Thomas theorem in research evaluation
Authors: Bornmann Lutz , Marx Werner 
Published: February 2020

AbstractThe well-known “Thomas theorem” in sociology is defined as follows: “if men define situations as real, they are real in their consequences” (Thomas and Thomas, The child in America, Knopf, Oxford, 1928, p. 572). The theorem focuses on “objective consequences of human subjectivity” (Sztompka, Robert K. Merton: An intellectual profile, Macmillan Education, Limited, London, 1986, p. 229). In this Letter to the Editor, we transfer the thought content of the Thomas theorem to university rankings: if rank positions between two universities define performance differences as real, they are real in their consequences (although the university ranking shows only slight differences between the universities’ scores).
Ice Giant Circulation Patterns: Implications for Atmospheric Probes
Authors: Fletcher Leigh N. , de Pater Imke , Orton Glenn S. , Hofstadter Mark D. , Irwin Patrick G. J. , Roman Michael T. , Toledo Daniel 
Published: February 2020

AbstractAtmospheric circulation patterns derived from multi-spectral remote sensing can serve as a guide for choosing a suitable entry location for a future in situ probe mission to the Ice Giants. Since the Voyager-2 flybys in the 1980s, three decades of observations from ground- and space-based observatories have generated a picture of Ice Giant circulation that is complex, perplexing, and altogether unlike that seen on the Gas Giants. This review seeks to reconcile the various competing circulation patterns from an observational perspective, accounting for spatially-resolved measurements of: zonal albedo contrasts and banded appearances; cloud-tracked zonal winds; temperature and para-H2 measurements above the condensate clouds; and equator-to-pole contrasts in condensable volatiles (methane, ammonia, and hydrogen sulphide) in the deeper troposphere. These observations identify three distinct latitude domains: an equatorial domain of deep upwelling and upper-tropospheric subsidence, potentially bounded by peaks in the retrograde zonal jet and analogous to Jovian cyclonic belts; a mid-latitude transitional domain of upper-tropospheric upwelling, vigorous cloud activity, analogous to Jovian anticyclonic zones; and a polar domain of strong subsidence, volatile depletion, and small-scale (and potentially seasonally-variable) convective activity. Taken together, the multi-wavelength observations suggest a tiered structure of stacked circulation cells (at least two in the troposphere and one in the stratosphere), potentially separated in the vertical by (i) strong molecular weight gradients associated with cloud condensation, and by (ii) transitions from a thermally-direct circulation regime at depth to a wave- and radiative-driven circulation regime at high altitude. The inferred circulation can be tested in the coming decade by 3D numerical simulations of the atmosphere, and by observations from future world-class facilities. The carrier spacecraft for any probe entry mission must ultimately carry a suite of remote-sensing instruments capable of fully constraining the atmospheric motions at the probe descent location.
Trends and Inequality in the New Active Ageing and Well-Being Index of the Oldest Old: a Case Study of Sweden
Authors: Fritzell Johan , Lennartsson Carin , Zaidi Asghar 
Published: February 2020

AbstractThe policy discourse on active ageing and well-being at the European level tends to have a strong focus on the experiences of the ‘young old’. In this study the focus instead is on the oldest old (75 years and older). The theoretical framework is inspired by the Active Ageing Index and the Nordic welfare research tradition. Active ageing and well-being indicators and domains of high relevance for the oldest old are used and a new Active Ageing-Well Being Index (AA-WB Index) is developed. Our aim is to go beyond averages and analyse changes over time and inequality in the AA-WB Index. The prime data is derived from two waves, 2004 and 2014, of the Swedish Panel Study of Living Conditions of the Oldest Old (SWEOLD), a nationally representative sample of older people. The results show an overall improvement in most domains of the AA-WB index, especially in the indicator participation in cultural and leisure activities. The findings also show clear and consistent gender and educational inequalities. In addition, the different domains correlate, implying that inequality within a domain is aggravated by the inequality in another domain. The study highlights that measurements on active ageing and well-being should place a greater importance on the living conditions of the oldest old with a special focus on inequality.
Whole-Brain Monosynaptic Inputs to Hypoglossal Motor Neurons in Mice
Authors: Guo Han , Yuan Xiang-Shan , Zhou Ji-Chuan , Chen Hui , Li Shan-Qun , Qu Wei-Min , Huang Zhi-Li 
Published: February 2020

AbstractHypoglossal motor neurons (HMNs) innervate tongue muscles and play key roles in a variety of physiological functions, including swallowing, mastication, suckling, vocalization, and respiration. Dysfunction of HMNs is associated with several diseases, such as obstructive sleep apnea (OSA) and sudden infant death syndrome. OSA is a serious breathing disorder associated with the activity of HMNs during different sleep–wake states. Identifying the neural mechanisms by which the state-dependent activities of HMNs are controlled may be helpful in providing a theoretical basis for effective therapy for OSA. However, the presynaptic partners governing the activity of HMNs remain to be elucidated. In the present study, we used a cell-type-specific retrograde tracing system based on a modified rabies virus along with a Cre/loxP gene-expression strategy to map the whole-brain monosynaptic inputs to HMNs in mice. We identified 53 nuclei targeting HMNs from six brain regions: the amygdala, hypothalamus, midbrain, pons, medulla, and cerebellum. We discovered that GABAergic neurons in the central amygdaloid nucleus, as well as calretinin neurons in the parasubthalamic nucleus, sent monosynaptic projections to HMNs. In addition, HMNs received direct inputs from several regions associated with respiration, such as the pre-Botzinger complex, parabrachial nucleus, nucleus of the solitary tract, and hypothalamus. Some regions engaged in sleep–wake regulation (the parafacial zone, parabrachial nucleus, ventral medulla, sublaterodorsal tegmental nucleus, dorsal raphe nucleus, periaqueductal gray, and hypothalamus) also provided primary inputs to HMNs. These results contribute to further elucidating the neural circuits underlying disorders caused by the dysfunction of HMNs.
Outcome of mechanical circulatory support at the University Medical Centre Utrecht
Authors: Felix S. E. A. , Ramjankhan F. Z. , Buijsrogge M. P. , Jacob K. A. , Asselbergs F. W. , Oerlemans M. I. F. , Kirkels J. H. , van Laake L. W. , Oppelaar A. M. C. , Suyker W. J. L. , de Jonge N. 
Published: February 2020

Abstract
Background
The prevalence of heart failure (HF) is increasing substantially and, despite improvements in medical therapy, HF still carries a poor prognosis. Mechanical circulatory support (MCS) by a continuous-flow left ventricular assist device (cf-LVAD) improves survival and quality of life in selected patients. This holds especially for the short-term outcome, but experience regarding long-term outcome is growing as the waiting time for heart transplantation is increasing due to the shortage of donor hearts. Here we present our results from the University Medical Centre Utrecht.

Methods
Data of all patients with a cf-LVAD implant between March 2006 and January 2018 were collected. The primary outcome was survival. Secondary outcomes included adverse events defined according to the Interagency Registry for Mechanically Assisted Circulatory Support (INTERMACS) definitions, described per patient year.

Results
A total of 268 patients (69% male, mean age 50 ± 13 years) received a cf-LVAD. After a median follow-up of 542 (interquartile range 205–1044) days, heart transplantation had been performed in 82 (31%) patients, the cf-LVAD had been explanted in 8 (3%) and 71 (26%) had died. Survival at 1, 3 and 5 years was 83%, 72% and 57%, respectively, with heart transplantation, cf-LVAD explantation or death as the end-point. Death was most often caused by neurological complications (31%) or infection (20%). Major bleeding occurred 0.51 times and stroke 0.15 times per patient year.

Conclusion
Not only short-term results but also 5‑year survival after cf-LVAD support demonstrate that MCS is a promising therapy as an extended bridge to heart transplantation. However, the incidence of several major complications still has to be addressed.
Identity, Connectedness, and Sexual Health in the Gay Sauna
Authors: Jaspal Rusi , Papaloukas Periklis 
Published: February 2020

Abstract
Introduction
This study focuses on the meanings and experiences that men living in Leicester, UK attribute to the gay sauna, the role of the gay sauna in their sense of identity, and how they construe their sexual behavior in this context.

Methods
In 2015, twenty male sauna users participated in a qualitative interview study. Data were analyzed using thematic analysis.

Results
The analysis yielded four themes: (1) identity authenticity in the gay sauna; (2) social connectedness among sauna users; (3) sexual risk and sexual health in the sauna; and (4) protecting the identity functions of the gay sauna. The themes show that engagement with the gay sauna can enhance feelings of identity authenticity and belongingness, and that individuals seek to protect these identity functions of the gay sauna despite recognizing the gay sauna as a “high-risk” environment. To protect identity, the notion of sexual risk is distanced from their own identities.

Discussion
Sauna users may experience better psychological health as a result of frequenting the sauna – it may promote identity authenticity and belongingness, and alleviate isolation, loneliness, and depression. The promotion of sexual health information and services in gay saunas may enable us to access individuals who might not ordinarily engage with sexual health services.

Social Policy Implications
There must be closer collaboration between sexual health services, sexual health outreach, and gay saunas in the area of sexual health, and identity concerns must be incorporated into the design of interventions.