bluuebunny/crossref_metadata_2025_split huggingface dataset:
The seed‐specific heat shock factor A9 regulates the depth of dormancy in Medicago truncatula seeds via ABA signalling
Authors: Zinsmeister Julia , Berriri Souha , Basso Denise Puntel , Ly‐Vu Benoit , Dang Thi‐Thu , Lalanne David , da Silva Edvaldo Aparecido Amaral , Leprince Olivier , Buitink Julia 
Published: July 2020

AbstractDuring the later stages of seed maturation, two key adaptive traits are acquired that contribute to seed lifespan and dispersal, longevity and dormancy. The seed‐specific heat shock transcription factor A9 is an important hub gene in the transcriptional network of late seed maturation. Here, we demonstrate that HSFA9 plays a role in thermotolerance rather than in ex situ seed conservation. Storage of hsfa9 seeds of Medicago truncatula and Arabidopsis had comparable lifespan at moderate storage relative humidity (RH), whereas at high RH, hsfa9 seeds lost their viability much faster than wild type seeds. Furthermore, we show that in M. truncatula, Mthsfa9 seeds acquired more dormancy during late maturation than wild type. Transient expression of MtHSFA9 in hairy roots and transcriptome analysis of Mthsfa9 Tnt1 insertion mutants identified a deregulation of genes involved in ABA biosynthesis, catabolism and signalling. Consistent with these results, Mthsfa9 seeds exhibited increased ABA levels and higher sensitivity to ABA. These data suggest that in legumes, HSFA9 acts as a negative regulator of the depth of seed dormancy during seed development via the modulation of hormonal balance.
A Spectroscopy‐based Methodology for Rapid Screening and Characterization of Phytochrome Photochemistry in Search of Pfr‐favored Variants
Authors: Antelo Giuliano Tomás , Sánchez‐Lamas Maximiliano , Goldbaum Fernando Alberto , Otero Lisandro Horacio , Bonomi Hernán Ruy , Rinaldi Jimena 
Published: July 2020

AbstractPhytochromes are photosensitive proteins with a covalently bound open‐chain chromophore that can switch between two principal states: red light absorbing Pr and far‐red light absorbing Pfr. Our group has previously shown that the bacteriophytochrome from Xanthomonas campestris pv. campestris (XccBphP) is a bathy‐like phytochrome that uses biliverdin IXα as a co‐factor and is involved in bacterial virulence. To date, the XccBphP crystal structure could only be solved in the Pr state, while the structure of its Pfr state remains elusive. The aims of this work were to develop an efficient screening methodology for the rapid characterization and to identify XccBphP variants that favor the Pfr form. The screening approach developed here consists in analyzing the UV‐Vis absorption behavior of clarified crude extracts containing recombinant phytochromes. This strategy has allowed us to quickly explore over a hundred XccBphP variants, characterize multiple variants and identify Pfr‐favored candidates. The high‐quality data obtained enabled not only a qualitative, but also a quantitative characterization of their photochemistry. This method could be easily adapted to other phytochromes or other photoreceptor families.
Performance Management Meets Red Tape: Bounded Rationality, Negativity Bias, and Resource Dependence
Authors: Hong Sounman 
Published: July 2020

AbstractGovernments around the world have implemented reforms to reduce red tape, but little evidence exists about whether they have achieved their goals. Utilizing a quasi‐experimental regression discontinuity design, this research examines the impact of a policy implemented by the Korean central government to reduce local levels of regulatory red tape. The findings show, first, that the centrally designed reform significantly reduced local levels of red tape, but the reduction occurred only among low‐performing localities. This supports the claim that organizations’ responses to positive and negative performance information are asymmetric—the negativity bias hypothesis. This finding is explained by the bounded rationality view of organizational decision‐making. Second, the impact was clearest among localities with high fiscal dependence on the central government. This supports the resource dependence hypothesis, which postulates that a policy's impact depends on the power imbalance between localities and the central government.
Photoprotection by mitochondrial alternative pathway is enhanced at heat but disabled at chilling
Authors: Li Yu‐Ting , Liu Mei‐Jun , Li Ying , Liu Peng , Zhao Shi‐Jie , Gao Hui‐Yuan , Zhang Zi‐Shan 
Published: July 2020

SUMMARYThe mitochondrial alternative pathway (AP) represents an important photoprotective mechanism for the chloroplast, but the temperature sensitivity of its photoprotective role is unknown. In this study, using the aox1a Arabidopsis mutant, the photoprotective role of the AP was verified under various temperatures, and the mechanism underlying the temperature sensitivity of the AP’s photoprotective role was clarified. It was observed that the photoprotective role of the AP increased with rising temperature but was absent at low temperature. The photoprotective role of the AP was severely reduced under non‐photorespiratory conditions. Disturbance of the AP inhibited the conversion of glycine to serine in mitochondria, which may restrain upstream photorespiratory metabolism and aggravate photoinhibition. With rising temperatures, photorespiration accelerated and the restraint of photorespiration caused by disturbance of the AP also increased, determining the temperature sensitivity of the AP’s photoprotective role. We also verified that not only the AP but also the cytochrome pathway in mitochondria contributes to photoprotection by maintaining photorespiration.
Isolation, identification and associated risk factors of non‐tuberculous mycobacteria infection in humans and dromedary camels in Samburu County, Kenya
Authors: Asaava Lucas L. A. , Githui Willie A. , Mwangi Moses , Mwangi Edwin , Juma Ernest , Moraa Ruth , Halakhe Adan , Gicheru Michael M. 
Published: July 2020

AbstractNon‐tuberculous mycobacteria are of public health significance, and zoonotic infection is attributed to the sociocultural practice of consumption of raw milk and the close human–livestock contact in pastoral communities. This study aimed at isolation, identification of mycobacteria from human sputum and camel milk and risk factors assessment in Samburu East, Kenya. Six hundred and twelve camels and 48 people presumed to have tuberculosis (TB) from 86 households in Wamba and Waso regions were screened. Camels were categorized into Somali, Turkana and Rendile breeds. Single intradermal comparative tuberculin test (SICTT) was used as a herd‐screening test on lactating camels and a milk sample collected from reactive camels. Sputum samples were collected from eligible members of participating households. A standard questionnaire on possible risk factors for both humans and camels was administered to respective household heads or their representatives. Total camel skin test reactors were 238/612 (38.9%). Milk and sputum samples were analysed at KEMRI/TB research laboratory for microscopy, GeneXpert®, culture and identification. Isolates were identified using 16S rRNA gene sequencing at Inqaba biotec in South Africa. Sixty‐four isolates were acid‐fast bacilli (AFB) positive of which M. fortuitum (3), M. szulgai (20), M. monacense (5), M. lehmanni (4), M. litorale (4), M. elephantis (3), M. duvalii (3), M. brasiliensis (1), M. arcueilense (1) and M. lentiflavum (1) were from milk; M. fortuitum (1), M. szulgai (2) and M. litorale (1) were from humans. Risk factors included the following: Turkana breed (OR = 3.4; 95% CI: 1.2–9.3), replacements from outside the County (OR = 2.1; 95% CI: 0.3–12.3), presence of other domestic species (small stock; OR = 4.6) and replacement from within the herd (OR = 3.2; 95% CI: 0.7–14.7). Zoonotic risk practices included raw milk consumption, shared housing and handling camels. Monitoring of zoonotic NTM through surveillance and notification systems is required.
Conceptual Framework Development for a Double-Walled Aortic Stent-Graft to Manage Blood Pressure
Authors: Kizilski Shannen B. , Amili Omid , Coletti Filippo , Faizer Rumi , Barocas Victor H. 
Published: July 2020

Abstract
A double-walled stent-graft (DWSG) design with a compressible gas layer was conceived with the goal of treating hypertension in patients receiving an aortic stent-graft. Early prototypes were developed to evaluate the design concept through static measurements from a finite element (FE) model and quasi-static inflation experiments, and through dynamic measurements from an in vitro flow loop and the three-element Windkessel model. The amount of gas in the gas layer and the properties of the flexible inner wall were the primary variables evaluated in this study. Properties of the inner wall had minimal effect on DWSG behavior, but increased gas charge led to increased fluid capacitance and larger reduction in peak and pulse pressures. In the flow loop, placement of the DWSG decreased pulse pressure by over 20% compared to a rigid stent-graft. Capacitance measurements were consistent across all methods, with the maximum capacitance estimated at 0.07 mL/mmHg for the largest gas charge in the 15 cm long prototype. Windkessel model predictions for in vivo performance of a DWSG placed in the aorta of a hypertensive patient showed pulse pressure reduction of 14% compared to a rigid stent-graft case, but pressures never returned to unstented values. These results indicate that the DWSG design has potential to be developed into a new treatment for hypertensive patients requiring an aortic intervention.
The International Diet-Health Index: a novel tool to evaluate diet quality for cardiometabolic health across countries
Authors: Wang Jifan , Masters William A , Bai Yan , Mozaffarian Dariush , Naumova Elena N , Singh Gitanjali M 
Published: July 2020

IntroductionDiet is a major modifiable risk factor for cardiometabolic disease; however, interpretable measures capturing impacts of overall diet on health that can be easily used by policymakers at the global/national levels are not readily available.MethodsWe developed the International Diet-Health Index (IDHI) to measure health impacts of dietary intake across 186 countries in 2010, using age-specific and sex-specific data on country-level dietary intake, effects of dietary factors on cardiometabolic diseases and country-specific cardiometabolic disease profiles. The index encompasses the impact of 11 foods/nutrients on 12 cardiometabolic diseases, the mediation of health effects of specific dietary intakes through blood pressure and body mass index and background disease prevalence in each country–age–sex group. We decomposed the index into IDHIbeneficial for risk-reducing factors, and IDHIadverse for risk-increasing factors. The flexible functional form of the IDHI allows inclusion of additional risk factors and diseases as data become available.ResultsBy sex, women experienced smaller detrimental cardiometabolic effects of diet than men: (females IDHIadverse range: −0.480 (5th percentile, 95th percentile: −0.932, –0.300) to −0.314 (−0.543, –0.213); males IDHIadverse range: (−0.617 (−1.054, –0.384) to −0.346 (−0.624, –0.222)). By age, middle-aged adults had highest IDHIbeneficial (females: 0.392 (0.235, 0.763); males: 0.415 (0.243, 0.949)) and younger adults had most extreme IDHIadverse (females: −0.480 (−0.932, –0.300); males: −0.617 (−1.054, –0.384)). Regionally, Central Latin America had the lowest IDHIoverall (−0.466 (−0.892, –0.159)), while Southeast Asia had the highest IDHIoverall (0.272 (−0.224, 0.903)). IDHIoverall was highest in low-income countries and lowest in upper middle-income countries (−0.039 (−0.317, 0.227) and −0.146 (−0.605, 0.303), respectively). Among 186 countries, Honduras had lowest IDHIoverall (−0.721 (−0.916, –0.207)), while Malaysia had highest IDHIoverall (0.904 (0.435, 1.190)).ConclusionIDHI encompasses dietary intakes, health effects and country disease profiles into a single index, allowing policymakers a useful means of assessing/comparing health impacts of diet quality between populations.
Outcomes important to patients with non-infectious posterior segment-involving uveitis: a qualitative study
Authors: Tallouzi Mohammad O , Moore David J , Bucknall Nicholas , Murray Philip I , Calvert Melanie J , Denniston Alastair K , Mathers Jonathan M 
Published: July 2020

ObjectiveUveitis, a group of disorders characterised by intraocular inflammation, causes 10%–15% of total blindness in the developed world. The most sight-threatening forms of non-infectious uveitis are those affecting the posterior segment of the eye, collectively known as posterior segment-involving uveitis (PSIU). Numerous different clinical outcomes have been used in trials evaluating treatments for PSIU, but these may not represent patients’ and carers’ concerns. Therefore, the aims of this study were to understand the impact of PSIU on adult patients’ and carers’ lives and to explore what outcomes of treatment are important to them.Methods and AnalysisFour focus group discussions were undertaken to understand the perspectives of adult patients (=18) and carers (10) with PSIU. Participants were grouped according to whether or not their uveitis was complicated by the sight-threatening condition uveitic macular oedema. Discussions were audio-recorded, transcribed and analysed using the framework analytical approach. Outcomes were identified and grouped into outcome domains.ResultsEleven core domains were identified as important to patients and carers undergoing treatment for PSIU, comprising (1) visual function, (2) symptoms, (3) functional ability, (4) impact on relationships, (5) financial impact, (6) psychological morbidity and emotional well-being, (7) psychosocial adjustment to uveitis, (8) doctor/patient/interprofessional relationships and access to healthcare, (9) treatment burden, (10) treatment side effects, and (11) disease control.ConclusionThe domains identified represent patients’ and carers’ experience and perspectives and can be used to reflect on outcomes assessed in PSIU. They will directly inform the development of a core outcome set for PSIU clinical trials.
Omental torsion associated with splenic torsion in a dog
Authors: Angelou Vasileia , Chatzimisios Kyriakos , Patsikas Michael , Psalla Dimitra , Papazoglou Lysimachos G 
Published: July 2020

SUMMARYA 5‐year‐old female spayed Cane Corso was referred for further investigation and treatment of a splenic torsion and an abdominal mass. The dog presented with a history of anorexia, depression and vomiting of 4 days’ duration. Physical examination revealed pain on abdominal palpation. On a plain radiograph of the abdomen, a cranial abdominal mass and splenomegaly were present. On CT images of the abdomen, a twisted splenic pedicle, splenic torsion and a well‐defined mass in the left cranial abdomen were observed adjacent and caudal to the greater curvature of the stomach. The dog underwent a midline coeliotomy, a total splenectomy and a partial gastrectomy to resect the mass attached to the great curvature of the stomach. Omental torsion was diagnosed on gross and histological examinations of the mass. The dog was reported by the owners to be free of clinical signs 2 years after surgery.
Search for long-lived heavy neutrinos at the LHC with a VBF trigger
Authors: Jones-Pérez J. , Masias J. , Ruiz-Álvarez J. D. 
Published: July 2020

AbstractThe charged current production of long-lived heavy neutrinos at the LHC can use a prompt charged lepton for triggering the measurement of the process. However, in order to fully characterize the heavy neutrino interactions, it is necessary to also probe Higgs or Z mediated neutral current production. In this case the charged lepton is not available, so other means of triggering are required. In this work, we explore the possibility of using a vector boson fusion trigger in the context of a GeV-scale Type I Seesaw model. We consider a minimal model, where both Higgs and Z-mediated contributions produce one heavy neutrino, as well as an extended model where the Higgs can decay into two heavy ones. Both scenarios are tested through displaced dilepton and displaced multitrack jet searches.
Cosmological bouncing solutions in f(T, B) gravity
Authors: Caruana Maria , Farrugia Gabriel , Levi Said Jackson 
Published: July 2020

AbstractTeleparallel Gravity offers the possibility of reformulating gravity in terms of torsion by exchanging the Levi-Civita connection with the Weitzenböck connection which describes torsion rather than curvature. Surprisingly, Teleparallel Gravity can be formulated to be equivalent to general relativity for a appropriate setup. Our interest lies in exploring an extension of this theory in which the Lagrangian takes the form of f(T, B) where T and B are two scalars that characterize the equivalency with general relativity. In this work, we explore the possible of reproducing well-known cosmological bouncing scenarios in the flat Friedmann–Lemaître–Robertson–Walker geometry using this approach to gravity. We study the types of gravitational Lagrangians which are capable of reconstructing analytical solutions for symmetric, oscillatory, superbounce, matter bounce, and singular bounce settings. These new cosmologically inspired models may have an effect on gravitational phenomena at other cosmological scales.
Refractive displacement of the radio-emission footprint of inclined air showers simulated with CoREAS
Authors: Schlüter Felix , Gottowik Marvin , Huege Tim , Rautenberg Julian 
Published: July 2020

AbstractThe footprint of radio emission from extensive air showers is known to exhibit asymmetries due to the superposition of geomagnetic and charge-excess radiation. For inclined air showers a geometric early-late effect disturbs the signal distribution further. Correcting CoREAS simulations for these asymmetries reveals an additional disturbance in the signal distribution of highly inclined showers in atmospheres with a realistic refractive index profile. This additional apparent asymmetry in fact arises from a systematic displacement of the radio-emission footprint with respect to the Monte-Carlo shower impact point on the ground. We find a displacement of $$\sim 1500$$∼1500 m in the ground plane for showers with a zenith angle of $$85^{\circ }$$85∘, illustrating that the effect is relevant in practical applications. A model describing this displacement by refraction in the atmosphere based on Snell’s law yields good agreement with our observations from CoREAS simulations. We thus conclude that the displacement is caused by refraction in the atmosphere.
Application of direct extended modified algebraic method of Bogoyavlenskii equation on lower and upper bounds in managing and optimizing queues
Authors: Akuamoah Saviour Worlanyo , Seadawy Aly R. 
Published: July 2020

The common queueing problem has always focused on domestic and industrialized activities. Various improved models of queueing theory are widely used to solve problems. The aim of this paper is to deduce the exact solution of Bogoyavlenskii equation via direct extended modified algebraic method. In addition, we apply it to determine the upper and lower bounds through the semidefinite optimization packages software (SeDuMi). The suggested model indicated strong bounds in reasonable times, we obtain a definite value of [Formula: see text] of the function [Formula: see text] over [Formula: see text] where [Formula: see text] in a time duration less than 60 s and a maximum value of [Formula: see text] where [Formula: see text] in a time frame of approximately 7 min. This study enriches the theoretical optimization queueing network and provides an analysis and decision making method for perfecting the theory.
CHD4 Promotes Breast Cancer Progression as a Coactivator of Hypoxia-Inducible Factors
Authors: Wang Yijie , Chen Yan , Bao Lei , Zhang Bo , Wang Jennifer E. , Kumar Ashwani , Xing Chao , Wang Yingfei , Luo Weibo 
Published: July 2020

Abstract

Recruitment of RNA polymerase II to hypoxia-inducible factor (HIF) target genes under normoxia is a prerequisite for HIF-mediated transactivation. However, the underlying mechanism of this recruitment remains unknown. Here we report that chromodomain helicase DNA-binding protein 4 (CHD4) physically interacts with α and β subunits of HIF1 and HIF2 and enhances HIF-driven transcriptional programs to promote breast cancer progression. Loss of HIF1/2α abolished CHD4-mediated breast tumor growth in mice. In breast cancer cells under normoxia, CHD4 enrichment at HIF target gene promoters increased RNA polymerase II loading through p300. Hypoxia further promoted CHD4 binding to the chromatin via HIF1/2α, where CHD4 in turn enhanced recruitment of HIF1α, leading to HIF target gene transcription. CHD4 was upregulated and correlated with HIF target gene expression in human breast tumors; upregulation of CHD4 and other known HIF coactivators in human breast tumors was mutually exclusive. Furthermore, CHD4 was associated with poor overall survival of patients with breast cancer. Collectively, these findings reveal a new fundamental mechanism of HIF regulation in breast cancer, which has clinical relevance.

Significance:
This study identifies CHD4 as a HIF coactivator and elucidates the fundamental mechanism underlying CHD4-mediated HIF transactivation in breast tumors.
Glomerular Hyperfiltration and Cancer: A Nationwide Population-Based Study
Authors: Kim Yaerim , Lee Soojin , Lee Yeonhee , Kang Min Woo , Park Sehoon , Park Sanghyun , Han Kyungdo , Paek Jin Hyuk , Park Woo Yeong , Jin Kyubok , Han Seungyeup , Han Seung Seok , Lee Hajeong , Lee Jung Pyo , Joo Kwon Wook , Lim Chun Soo , Kim Yon Su , Kim Dong Ki 
Published: July 2020

Abstract

Background:
Glomerular hyperfiltration is associated with all-cause mortality. Herein, we evaluated the association between glomerular hyperfiltration and the development of cancer, the most common cause of death, in an Asian population.

Methods:
We retrospectively reviewed the National Health Insurance Service database of Korea for people who received national health screenings from 2012 to 2013. Glomerular hyperfiltration was defined as the 95th percentile and greater after stratification by sex and age decile. We performed a multivariate Cox regression analysis using glomerular hyperfiltration at the first health screening as the exposure variable and cancer development as the outcome variable to evaluate the impact of glomerular hyperfiltration on the development of cancer.

Results:
A total of 1,953,123 examinations for patients with a median follow-up time of 4.4 years were included in this study. Among the 8 different site-specific cancer categories, digestive organs showed significant associations between glomerular hyperfiltration and cancer. The population with glomerular hyperfiltration showed an increased risk for stomach cancer [adjusted hazard ratio (aHR) = 1.22], colorectal cancer (aHR = 1.16), and liver or intrahepatic malignancy (aHR = 1.35).

Conclusions:
Glomerular hyperfiltration was associated with an increased risk for the development of cancer in specific organs, such as the stomach, colorectum, and liver and intrahepatic organ.

Impact:
Glomerular hyperfiltration needs to be considered a significant sign of the need to evaluate the possibility of hidden adverse health conditions, including malignancies.