bluuebunny/crossref_metadata_2025_split huggingface dataset:
310 Using synergistic antibacterial combinations based on vegetable essential oils to replace feeding antibiotics
Authors: Hu yanping , Huang Jie , Zhou Zhongxin 
Published: December 2019

Abstract
Vegetable essential oils, medium-chain fatty acids and organic acids have great potential in the development of alternatives to feeding antibiotics, but many problems in practice limit their application, such as irritating odor, poor water solubility, easy oxidation and volatility. In this study, we found that vegetable essential oils (carcilol, cinnamaldehyde, terptol-4) and medium chain fatty acids (octanoic acid and nonanoic acid) or organic acids (citric acid) had significantly synergistic bactericidal effects on enterotoxigenic Escherichia coli and Salmonella enteribacilli, which are both common pathogens causing piglets diarrhea. Furthermore, we also compared the tolerances of the enterotoxigenic Escherichia coli against plant essential oils (cinnamaldehyde, carcocol, 4- terptol), medium chain fatty acids (octanoic acid and nonylic acid), organic acids (citric acid), and commonly used antibiotic growth promoter (gentamycin sulfate). They were in vitro cultured with ETEC strain for 30 consecutive generations and the minimum inhibitory concentration (MIC) value was detected. The results showed that the MIC values of carvonol, cinnamaldehyde, terpineol-4, octanoic acid, nonylic acid, and citric acid against the ETEC bacteria increased by 5, 3, 1, 0.3, 0.4 and 0 times. But the MIC value of gentamicin against ETEC bacteria increased, in the 20th generation, by more than 64 times. In conclusions, the synergistic bactericidal combination based on the plant essential oil and middle chain fatty acid or organic acid may be a better strategy to replace feeding antibiotics because their synergistic bactericidal combination can greatly reduce the effective concentration of plant essential oil, medium chain fatty acid and organic acid, thus alleviating to a certain extent the shortcomings of unstable effects caused by the irritating odor, the poor water solubility, easy oxidation and volatility. The synergistic bactericidal combination based on plant essential oils are also relatively difficult to cause bacterial tolerance and alleviate bacterial resistance to a certain extent.
8 Precision dairy cattle welfare: use of technology for individual measures, record keeping and early disease detection
Authors: Costa Joao 
Published: December 2019

Abstract
Precision Dairy Farming (PDF) technologies measure physiological or behavioral parameters from individual cows with the objective of enhancing animal health and welfare. Continuous monitoring of individual animals allows for detailed data record keeping and offers an opportunity to tailor management to the individual. Here we present some of the available technologies related to some of the key animal welfare measures on dairy farms, such as lying and rumination behavior, lameness and BCS monitoring. Generally, changes associated with lying time and rumination behavior are precise at the individual level but still not able to be generalized between herds, regions and productions systems. Automated gait scoring and BCS of individual cows are commercially available and becoming more accessible, allowing for increased opportunity for use on commercial dairy farms and in dairy research. Any use of PDT should understand the precision, accuracy and bias of the devices used, especially when interpreting herd summaries and regional averages. Most technologies evaluated are highly precise, but many fail to be accurate. In addition to their uses for farmers, PDT with high accuracy also aid researchers and processors to collect comprehensive data sets and reliable records. Overall, the potential uses of precision technology tools are likely to become more common on farms, and consequently improve dairy cattle management strategies, aid in herd monitoring, and improve animal welfare at the individual level.
204 Relationships between semen quality and scrotal circumference, adiposity, and rumen conditions of developing bulls
Authors: Proctor Jarret A , Long Nathan S , Biehler Connor K , Schneider Liesel G , Rhinehart Justin D , Smith Jason K 
Published: December 2019

Abstract
Growing concern over breeding soundness examination failures has led to increased interest in identifying factors that may influence semen quality of developing bulls. An experiment was conducted to evaluate relationships between semen quality and scrotal circumference, adiposity, and rumen conditions of Angus bulls (n = 48; 332 ± 47 d of age) that were developed over an 84-d period. Bulls underwent breeding soundness examinations at each of four 21-d intervals. Backfat thickness was measured on d 0 and 84, and rumen pH and temperature were continuously monitored throughout the experiment via rumen boluses (eBolus; eCow, Devon, UK). Simple linear regression was conducted (JMP 13.0; SAS Institute Inc., Cary, NC) to determine independent relationships between semen characteristics and respective explanatory variables. Change in scrotal circumference between d 0 and 84 was directly related to change in percent morphologically-normal sperm (R2 = 0.12; P &lt; 0.05), but no other semen quality characteristics (P ≥ 0.17). Change in backfat thickness throughout the experiment tended to be inversely related to change in percent morphologically-normal sperm (R2 = 0.06; P &lt; 0.10), but was directly related to changes in semen volume (R2 = 0.10; P &lt; 0.05) and total sperm per ejaculate (R2 = 0.12; P &lt; 0.05), but not change in motility (P = 0.24). Mean rumen pH tended to be directly related to changes in semen volume (R2 = 0.07; P &lt; 0.10) and total sperm per ejaculate (R2 = 0.07; P &lt; 0.10), but not changes in motility (P = 0.28) or percent morphologically-normal sperm (P = 0.94). Mean rumen temperature was not related to any semen quality characteristics (P ≥ 0.15). Collectively, these results suggest that rumen pH, as well as changes in scrotal circumference and adiposity are related to and independently explain variation in semen quality of developing bulls.
205 Runs of homozygosity and analysis of inbreeding depression
Authors: Sumreddee Pattarapol , Toghiani Sajjad , Roberts Andrew J , Hay El H , Aggrey Samuel E , Rekaya Romdhane 
Published: December 2019

Abstract
Pedigree information was traditionally used to assess inbreeding. Availability of high-density marker panels provides an alternative to assess inbreeding, particularly in the presence of incomplete and error-prone pedigrees. Assessment of autozygosity across chromosomal segments using runs of homozygosity (ROH) is emerging as a valuable tool to estimate inbreeding due to its general flexibility and ability to quantify chromosomal contribution to genome-wide inbreeding. Unfortunately, identifying ROH segments is sensitive to the parameters used during the search process. These parameters are heuristically set, leading to significant variation in the results. The minimum length required to identify a ROH segment has major effects on the estimation of inbreeding, yet it is arbitrarily set. Understanding the rise, purging, and the effects of deleterious mutations requires the ability to discriminate between ancient and recent inbreeding. However, thresholds to discriminate between short and long ROH segments are largely unknown. To address these questions, an inbred Hereford cattle population of 785 animals genotyped for 30,220 SNPs was used. A search algorithm to approximate mutation loads was used to determine the minimum length of ROH segments. It consisted of finding genome segments with significant differences in trait means between animals with high and low autozygosity intervals at certain threshold values. The minimum length was around 1 Mb for weaning and yearling weights and ADG, and 2.5 Mb for birth weight. Using a model-based clustering algorithm, a mixture of three Gaussian distributions was clearly separable, resulting in three classes of short (&lt; 6.16 Mb), medium (6.16–12.57 Mb), and long (&gt;12.27 Mb) ROH segments, representing ancient, intermediate, and recent inbreeding. Contribution of ancient, intermediate and recent to genome-wide inbreeding was 37.4%, 40.1% and 22.5%, respectively. Inbreeding depression analyses showed a greater damaging effect of recent inbreeding, likely due to purging of old highly deleterious haplotypes.
215 Selection response for meat quality and carcass composition in commercial pigs using crossbred and purebred traits as predictors
Authors: Lozada-Soto Emmanuel A , Fix Justin , Schwab Clint , Tiezzi Francesco 
Published: December 2019

Abstract
Meat quality (MQ) and carcass composition (CC) are traits with high economic value, but their phenotyping costs makes direct selection for these traits economically unsustainable. Evaluation of traits with lower cost of collection in crossbred (CB) and purebred (PB) animals for use in selection for MQ and CC remains limited. The aim of this study was to measure the response to selection of economically important MQ and CC traits using different selection indices built on cost-effective correlated traits. Phenotypic and genomic information (using 60K SNP chips) was collected for three-way CB (n = 1227 to 5117 phenotyped, n = 1252 genotyped) and PB (n = 3,251 phenotyped, n = 1035 genotyped) Duroc animals belonging to 28 paternal half-sib families. The three breeding objectives (for which response was estimated) were intramuscular fat (IMF), slice shear force (SSF), and loin weight (LW). In total 7 different selection indices (A-G) were used to estimate the selection response for each breeding objective (Table 1). Heritability and genetic correlation parameters for all traits were estimated using GIBBS1F90. Selection response was obtained as expected response per generation weighed by the accuracy of breeding values estimated from a 4-fold cross validation, where animals were grouped based on sire relatedness. For all three breeding objectives, the index which provided best selection response was A (direct selection), with values of 0.35%, -0.72 kg, and 0.85 kg for IMF, SSF, and LW respectively. The index which provided the second-best selection response was B (color and pH traits) for all three breeding objectives, with values of 0.15%, -0.26kg, and 0.30kg for IMF, SSF, and LW respectively. Results for selection response using indices C-G can be found in Table 1. In the absence of direct selection due to high costs of phenotyping, respectable selection response can be achieved by indirect selection using traits with lower cost of collection.
220 Mitochondrial DNA polymorphism as a measure of genetic diversity in African tilapia fish
Authors: Ikpeme Ekei , Ekerette Ekerette , Ozoje Michael 
Published: December 2019

Abstract
This study focused on the assessment of genetic diversity in African tilapia fish (Oreochromis niloticus) obtained from two populations (Domita in South-South and Odeda in South-West, Nigeria) using the displacement loop (D-loop) and cytochrome B region of the mitochondrial deoxyribonucleic acid (mtDNA). A total of 28 samples (15 from South-South and 13 from South-West) were used for the genetic analysis. DNA was extracted from the tissue of all the samples using Quik-gDNATM miniPrep kit. The D-loop region was sequenced for all samples from the two populations, while cytochrome b (cyt b) region of mtDNA was only sequenced for samples from South-South population Multiple sequence alignment was carried out using molecular evolutionary genetic analysis (MEGA) software before subsequent genetic analyses. There were 176, 162 and 144 polymorphic sites for D-loop of South-South, South-West and cyt b of South-South populations, respectively. Haplotype diversities (Hd) were 1.00 ± 0.024 and 1.00 ± 0.030 while nucleotide diversities were 0.168 ± 0.086 and 0.161 ± 0.084 for D-loop of South-South and South-West populations, respectively. For the cyt b, haplotype and nucleotide diversities were 0.91 ± 0.003 and 0.051 ± 0.016. The genetic distance inferred between D-loop regions of South-South and South-West populations was 0.243. Phylogenetic analysis grouped the samples into two clusters based on population. Analysis of molecular variance (AMOVA) revealed 37.32% variation within population and 62.68% variation among population with a significant fixation index of 0.627 (P &lt; 0.05). The findings of this study revealed high level of genetic diversity among the tilapia populations and may serve as pivot information for the genetic improvement of this important species.
56 Redox regulation of stallion spermatozoa and its impact in reproductive technologies
Authors: Vega Fernando J Pena 
Published: December 2019

Abstract
Redox regulation is being unveiled as a major mechanism regulating sperm functionality, probably at the same level as tyrosine phosphorylation. Sophisticated mechanisms must be present to maintain redox status under physiological control. The spermatozoa has antioxidant defenses, including glutathione, and other enzymatic antioxidant defenses such as thioredoxin and peroxiredoxin families of proteins. Oxidative stress is thus better defined as the fail in the regulation of redox signalling due either overproduction of ROS, or exhaustion of regulatory mechanisms. The stallion spermatozoa is a paradigm of this; recent research has shown apparently paradoxical results, in this regard more fertile spermatozoa show increased ROS production. A major component of the alteration of ROS homeostasis in cryopreserved spermatozoa is the exhaustion of intrinsic antioxidant defence mechanisms. Thiols and particularly glutathione (GSH) are essential for the regulation of stallion sperm functionality. As cysteine is a pre cursor of GSH, we hypothesized that stallion spermatozoa are able to synthesize this tri-peptide using exogenous cysteine. We investigated the presence of two enzymes required to synthesize GSH (GSS and GCLC); using western blotting and immunocytochemistry we detected both enzymes in stallion spermatozoa. The inhibition of glutamate cysteine ligase reduced the recovery of GSH by addition of cysteine after depletion, suggesting that stallion spermatozoa may use exogenous cysteine to regulate GSH. Mean concentration of GSH in stallion spermatozoa was 8.2±2.1 mM /109spermatozoa, well above the nanomolar ranges reported for other mammals. In addition we have reported the presence of the soluble carrier family 7 member 11 (SLC7A11) antiporter, which exchanges extracellular cystine (Cyss) for intracellular glutamate. The investigations of the author have been funded by Ministerio de Ciencia y Competividad- FEDER AGL 2017-83149-R and Junta de Extremadura FEDER IB16030 and GR18008
91 The production of sulfated 16-androstene steroids by porcine Leydig cells and their potential role in the development of boar taint
Authors: Bone Christine , Laderoute Heidi , Brewer Dyanne , Cameron Jocelyn , Squires E James 
Published: December 2019

Abstract
Boar taint is a meat quality issue characterized by an off-odour or off-flavour in pork caused primarily by the accumulation of androstenone in the fat, but sensory estimates of boar taint do not always correlate with fat androstenone concentrations. However, these evaluations examine the sensory qualities of both the fat and lean tissue of heated pork products. Sulfated metabolites of androstenone are polar compounds that are abundantly produced by the Leydig cells of the testes, which may accumulate in more hydrophilic lean tissue. Therefore, the purpose of this study was to investigate the testicular metabolism of androstenone, which is responsible for the high production of sulfated androstenone metabolites. Leydig cells were isolated from 7-month-old Yorkshire, Duroc and terminal cross [Duroc x (Landrace x Yorkshire)] (n = 3) boars and incubated with radiolabeled androstenone for 8 hours. The proportion of sulfated metabolites produced was quantified using reverse phase high performance liquid chromatography (HPLC) and radioisotope detection. The sulfated metabolites were subsequently identified using liquid chromatography-mass spectrometry (LC-MS/MS). Statistical analysis was conducted using a one-way ANOVA in SAS. Following isolation and analysis with LC-MS/MS, the sulfated metabolites were identified as androstenol-3-sulfate and two major sulfated forms of androstenone. Additionally, removal of the sulfate group from these two sulfated forms of androstenone returned the parent compound androstenone, and not a hydroxylated metabolite. The average production of sulfated androstenol produced across all boars was 52.1±8.6%, which was not significantly different (P = 0.7) from the average production of sulfated androstenone (47.9±8.6%). The results of this study indicate that androstenone is directly sulfated, which allows these sulfated metabolites to function as steroid reservoirs that can enzymatically regenerate free androstenone within hydrophilic lean tissue. Alternatively, these metabolites may accumulate in the lean tissue, which we are proposing as a novel mechanism that contributes to the development of boar taint.
102 The National Center for Applied Reproduction and Genomics (NCARG) in Beef Cattle: supporting improvements in reproductive management
Authors: Patterson David J , Brown Scott , Smith Michael F , Lamberson William R , Taylor Jeremy , Spencer Thomas E , Poock Scott E , Thomas Jordan M , Decker Jared E 
Published: December 2019

Abstract
Limited transfer of existing and emerging reproductive technologies to beef cattle enterprises precludes those enterprises from reaching their economic potential and places them at a competitive disadvantage relative to livestock enterprises where such technologies are being implemented more aggressively. On the other hand, and until now, the beef cattle sector has never had the reproductive tools available to facilitate widespread successful adoption of these technologies within the industry. Hence, the need for increased efforts to transfer technology to the industry has never been greater. The National Center for Applied Reproduction and Genomics (NCARG) in Beef Cattle will serve as a new model for research and technology transfer wherein industry participants at all levels and from across the US are afforded the opportunity to access information and acquire new skills that will improve reproductive management and resulting genetic makeup of our nation’s beef cattle industry. Comprehensive educational training programs will be developed for practicing veterinarians, extension livestock specialists, allied industry, producers, and students that will enable various segments of the beef cattle sector to acquire (through face-to-face and distance-learning applications) new knowledge and skills that will aid in the successful adoption of reproductive procedures/biotechnologies. Curriculum modules designed to support improvements in reproductive management will be comprised of the following: 1) Heifer development programs including reproductive tract scoring, fetal aging and sexing; 2) Breeding management, including estrus synchronization, artificial insemination, sex-sorted semen, and embryo transfer; 3) higher education student internships in reproductive management; and 4) on-farm beef heifer development and marketing programs patterned after the Missouri Show-Me-SelectTM Replacement Heifer Program. In addition, a graduate certificate program will be offered, entitled “Bovine Reproductive Management and Genomic Technologies.” Collectively, NCARG will serve as a new model for research and technology transfer intent on improving reproductive management and genetic makeup of our nation’s beef industry.
263 Interaction between stocking and supplementation rates on the performance of steers grazing mixed-grass prairie during winter
Authors: Gunter Stacey A 
Published: December 2019

Abstract
To evaluate the effects of stocking and supplementation rates on steers grazing mixed-grass prairie during winter, 16 pastures (10 to 21 ha each) were selected and treatments were arranged in a 3 x 2 factorial design; the first factor was an stocking rate of 39.4, 33.2, and 29.7 animal-unit-d/ha harvested over 88 d of grazing; the second factor was supplementation at 0.9 or 1.4 kg/d. Steers were fed a 43% CP cottonseed meal-based pellet. Pastures were stocked annually in late January (2009, 2010, and 2011) with British x Continental crossbred beef steers (body weight = 202 ± 6.7 kg; n = 137 steers/yr) at stocking densities described above. Data were analyzed by ANOVA with pasture as the experimental unit and year as a random variable; least-squares means were separated with linear and quadratic contrast. From late-January to mid-March, average daily gain (ADG, kg) did not differ (P = 0.13) among stocking rates, but ADG differed (P = 0.01) between 0.9 and 1.4 kg of supplement/d. From mid-March to late-April and over the entire grazing period, ADG responded to stocking rate and interacted (P &lt; 0.01) with supplementation rate; ADG tended to respond quadratically (P ≤ 0.09) to stocking rate at 0.9 kg/d of supplementation, but with 1.4 kg/d of supplement ADG was not affected (P ≥ 0.18). Body weight gain per hectare tended (P &lt; 0.08) to interact between stocking and supplementation rates. With 0.9 kg/d of supplement the BW gain per hectare increased quadratically (P &lt; 0.01) in response to increasing stocking rate, where supplementation at 1.4 kg/d resulted in BW gain per hectare increasing linearly (P &lt; 0.01). Optimal supplementation rates with high-protein feeds is related to stocking rate. At lower stocking rates, less supplement seems to be most beneficial. At higher stocking rates, more supplement is justified.
259 Screening of Lespedeza germplasm for forage quality and bioactivity
Authors: Terrill Thomas H , Morning Breyanna , Courson Erica , Morris Bradley , Naumann Harley , Whitley Niki , Owen Vicki 
Published: December 2019

Abstract
Interest in the condensed tannin-containing legume sericea lespedeza (Lespedeza cuneata) as a nutraceutical (bioactive) forage for livestock has been increasing in the United States but other Lespedeza species have not been adequately evaluated. A study was completed to determine the nutritional and bioactivity potential of Lespedeza species obtained from the USDA Plant Genetic Resources Conservation Unit in Griffin, GA. Accessions from 15 Lespedeza species were planted in small plots at Fort Valley State University in Fort Valley, GA. After establishment, forage was harvested, freeze-dried, ground and analyzed for neutral detergent fiber (NDF), acid detergent fiber (ADF), in vitro true digestibility (IVTD), total phenolics (TP), and protein precipitable phenolics (PPP). Species differences were significant (P &lt; 0.01) for all parameters measured. Lespedeza cuneata accessions, including “AUGrazer,” the cultivar most commonly used as an anti-parasitic crop in the United States had NDF, ADF, and IVTD values of 35.7±0.4%, 26.8±0.4%, and 74.3±1.2%, while L. virginica, a native species, had 34.9±3.0%, 26.0±2.5%, and 74.9±4.2%, respectively. The L. cuneata accessions averaged 179.6±4.3 and 133.6±3.5 mg/g of forage material for TP and PPP, while L. virginica averaged 564.2±24.6 and 306.5±19.9 mg/g, respectively. Other species varied from 30.5±3.0 to 47.4±4.2% NDF, 22.9±1.6 to 36.4±3.6% ADF, 55.2±5.9 to 81.6±3.4% IVTD, and 107.7±17.4 to 283.5±34.7 and 55.2±14.0 to 139.4±28.1 mg/g of forage for TP and PPP, respectively. Based upon these results, Lespedeza species tested in this study, particularly L. virginica, have potential as nutraceutical forages for livestock production systems. As L. cuneata accessions established and grew much better than other Lespedeza species in this study, agronomic testing of this germplasm collection in other environments is warranted. In addition, determining potential anti-parasitic properties of these species using in vitro and in vivo testing would be beneficial.