bluuebunny/crossref_metadata_2025_split huggingface dataset:
Appropriate timing of performing abdominal ultrasonography and termination of follow-up observation for antenatal grade 1 or 2 hydronephrosis
Authors: Nakane Akihiro , Mizuno Kentaro , Kato Taiki , Nishio Hidenori , Kamisawa Hideyuki , Kurokawa Satoshi , Maruyama Tetsuji , Yasui Takahiro , Hayashi Yutaro 
Published: January 2020

Abstract
BackgroundMost cases of antenatal the Society of Fetal Urology (SFU) grade 1 or 2 hydronephrosis improve or resolve spontaneously with conservative treatment. However, there is no consensus on the duration of follow-up for cases of grade 1 or 2 hydronephrosis. The aim of this study was to determine the need for continuous follow-up period and new management of children with antenatal grade 1 or 2 hydronephrosis.MethodsSubjects underwent ultrasonographic assessment for hydronephrosis according to the SFU classification. We retrospectively evaluated 112 patients with postnatal grade 1 hydronephrosis and 69 with grade 2 hydronephrosis hydronephrosis using abdominal ultrasonography between January 2010 and December 2017. We examined the change in hydronephrosis grade on repeat ultrasonography.ResultsThe mean follow-up duration was 44.9 months. Initial grade 1 hydronephrosis disappeared in 44.8% of cases at 12 months, 66.4% at 24 months and 73.2% at 48 months. Initial grade 2 hydronephrosis showed improvement in grade in 70.0% of cases at 12 months, 88.3% at 24 months and 89.5% at 48 months. However, 13.4% of grade 1 and 2.9% of grade 2 cases increased in grade and 99.3% of these cases worsened within the first 6 months. No cases with increased grade required pyeloplasty. Initial disappearance and later reappearance of hydronephrosis occurred in 13.4% of grade 1 cases and 2.9% of grade 2 cases. The mean duration of later reappearance of hydronephrosis was 6.9 months. No cases showed reappearance of hydronephrosis after more than 1 year. Conclusions Ultrasonography within the first 6 months was necessary for management of children with antenatal grade 1 or 2 hydronephrosis, because some patients showed worsening. Most cases of grade 1 or 2 hydronephrosis resolved spontaneously, however a few cases reappeared within 1 year. Therefore, ultrasonography after 1 year was necessary in children with hydronephrosis that spontaneously disappeared. The appropriate time to end the follow-up was considered to have been after one year or more has passed since the disappearance was confirmed.
Relationship of endoscopic lesions of the renal papilla with type of renal stone and 24 h urine chemistry
Authors: Arroyo Xavier Arnau Sabaté , Grases Felix , Quetglas Jose Luis Bauza , Garcia Jorge Guimera , Ayala Enrique Pieras 
Published: January 2020

Abstract
BACKGROUND: Our purpose was to study the relationships of the 3 different types of endoscopic calcifications of the renal papilla (Randall’s plaque, intratubular calcification, papillary crater) with the type of stone and urine chemistry.METHODS This prospective study examined 41 patients (age range: 18 to 80 years) who received retrograde intrarenal surgery (RIRS) for renal lithiasis (mean stone size: 15.3 ± 7.2 mm). Endoscopic injuries of the renal papilla were classified as Randall’s plaque, intratubular calcification, or papillary crater. Calculi were classified as uric acid, calcium oxalate monohydrate (COM; papillary and cavity), calcium oxalate dihydrate (COD), or calcium phosphate (CP). A 24 h urine analysis of calcium, oxalate, citrate, phosphate, and pH was performed in all patients. The relationship of each type of papillary endoscopic injury with type of stone and urine chemistry was determined. Fisher’s exact test and Student’s t-test were used to determine the significance of relationships, and a p value below 0.05 was considered significant. RESULTS The most common endoscopic injury was tubular calcification (78%), followed by Randall’s plaque (58%), and papillary crater (39%). There was no significant relationship of Randall’s plaque with type of stone. However, endoscopic intratubular calcification (p = 0.025) and papillary crater (p = 0.041) were more common in patients with COD and CP stones. There were also significant relationships of papillary crater with hypercalciuria (p = 0.036) and hyperoxaluria (p = 0.024), and of Randall’s plaque with hypocitraturia (p = 0.005).CONCLUSIONS There are certain specific relationships between the different types of endoscopic papillary calcifications with stone chemistry and urine chemistry. COD and CP stones were associated with endoscopic tubular calcifications and papillary craters. Hypercalciuria was associated with tubular calcification, and hypocitraturia was associated with Randall’s plaque.
Lenti-shRNA-mediated FAM54A knockdown suppresses the proliferation and induces apoptosis of human Burkitt lymphoma cells
Authors: Yang Guang , Wang Hua Xiao , Yan Bin , Xu Yan Chun , Zheng Yi Ru , Chen Xi Meng , Liu Fang Rui , Zhang Di Hai , Jin Shun Zai 
Published: January 2020

Abstract
Background Burkitt lymphoma is a kind of non-Hodgkin B-cell-derived malignancy, derived from germinal center B cells. FAM54A has been proved to be involved in various physiological and pathological processes of cancers, but the biological function of FAM54A in Burkitt lymphoma remains unclear. Thus, the aim of our research was to elucidate the roles of FAM54A in proliferation, apoptosis and cell cycle of Burkitt lymphoma.Methods Burkitt lymphoma cell line (Namalwa) was chosen to perform the following experiments. FAM54A-shRNA and negative control-shRNA lentivirus that were synthesized, respectively by Qiagen were used to transfect targeted cells in order to knockdown FAM54A or as negative control. Then, cell proliferation, cell cycle and cell apoptosis were detected by using MTS assay, propidium iodide staining and Annexin V-APC staining, respectively.Results Our results showed that high expression of FAM54A protein was found in Namalwa cell line. Furthermore, MTS analysis exhibited that knockdown of FAM54A obviously inhibited cell proliferation in Namalwa cells. What’s more, cell cycle analysis showed that knockdown of FAM54A induced Namalwa cell apoptosis and arrested cell cycle in G2/M phase.Conclusions These findings suggest that FAM54A is essential for Namalwa cell proliferation and may be a potential therapeutic target for the treatment of Burkitt lymphoma.
Etoposide-mediated interleukin-8 secretion from bone marrow stromal cells induces hematopoietic stem cell mobilization
Authors: Kang Ka-Won , Lee Seung-Jin , Kim Ji Hye , Lee Byung-Hyun , Kim Seok Jin , Park Yong , Kim Byung Soo 
Published: January 2020

Abstract
Background This study assessed the mechanism of hematopoietic stem cell (HSC) mobilization using etoposide with granulocyte-colony stimulating factor (G-CSF) and determined how it differed from that using cyclophosphamide with G-CSF or G-CSF alone.Methods The study analyzed data from 173 non-Hodgkin’s lymphoma patients who underwent autologous peripheral blood stem cell transplantation (auto-PBSCT), in vitro experiments using HSCs and bone marrow stromal cells (BMSCs), and in vivo mouse model studies.Results The etoposide with G-CSF mobilization group showed the highest yield of CD34+ cells and the lowest change in white blood cell counts during mobilization. Etoposide triggered interleukin (IL)-8 secretion from BMSCs and caused long-term BMSC toxicity, which were not observed with cyclophosphamide treatment. The expansion of CD34+ cells cultured in BMSC-conditioned medium containing IL-8 was more remarkable than that without IL-8. The expression of CXCR2, mTOR, and cMYC in HSCs was gradually enhanced at 1, 6, and 24 h after IL-8 stimulation. In animal studies, the etoposide with G-CSF mobilization group presented stronger expression of IL-8-related cytokines and MMP9 and scantier expression of SDF-1 in the bone marrow, compared to the other groups not treated with etoposide.Conclusion Collectively, the unique mechanism of etoposide with G-CSF-mediated mobilization is associated with the secretion of IL-8 from BMSCs, causing the enhanced proliferation and mobilization of HSCs in the bone marrow, which was not observed in the mobilization using cyclophosphamide with G-CSF or G-CSF alone. Moreover, the long-term toxicity of etoposide to BMSC emphasizes the need for further studies to develop more efficient and safe chemo-mobilization strategies.
Increased risk for development of severe cervical dysplasia among postmenopausal women with normal cytology and presence of HPV mRNA
Authors: Asciutto Katrin Christine , Borgfeldt Christer , Forslund Ola 
Published: January 2020

Abstract
Background: During 2013 and 2016 the Region of Skåne, Sweden, started to analyse human papillomavirus (HPV) and cytology in postmenopausal women 60-65 years of age. Our aim was to evaluate if the presence of high-risk (HR) HPV mRNA could predict the development of cervical abnormalities among HR-HPV DNA positive women with normal cytology. Methods: A total of 271 women, 60-65 years of age, underwent liquid based cytology (LBC) and HPV testing by using the HR-HPV DNA MGP-PCR-Luminex assay. HR-HPV DNA-positive women with normal cytology underwent complimentary HPV mRNA testing (Aptima, Hologic). Over a period of 49 months (SD 11.0) the women received regular follow-up at intervals of 12-18 months. Women with abnormal cytology and/or a positive HR-HPV result at two subsequent visits were scheduled for colposcopy and clinical examination. Results: Over the surveillance period, 3.6% (10/271) of the HR-HPV DNA positive women developed histologically confirmed high-grade squamous intraepithelial lesions (HSILs) or worse and 13.3% (36/271) were diagnosed with cervical atypical squamous cells of undetermined significance (ASCUS) or low-grade squamous intraepithelial lesions (LSILs). The cumulative incidence rates (CIR) were 29.7% (CI 24.8-30.1) for HSIL or worse among HPV mRNA-positive women at enrolment (39.4% 107/271) and 0% among HPV mRNA negative women (60.5%, 164/271), (p=0.002). The corresponding CIRs for ASCUS and LSIL was 59.9% (95% CI 59.3-66.2) and 26.1% (95 %CI 16.5-35.9), (p=0.001). Conclusions : Postmenopausal women with normal cytology along with presence of HR-HPV mRNA are at increased risk for development of severe cervical dysplasia, in contrast to those women with negative HR-HPV mRNA.
Epidemiology of nosocomial carbapenem-resistant Klebsiella pneumonia bloodstream infections in south China: factors related to the patient mortality
Authors: Xie Xiaona , Cai Xueding , Wan Tingting , Shao Lianyou , Chen Lijiang , Yao Dan , Ding Cheng , Li Guoping , Wang Liangxing , Huang Xiaoying 
Published: January 2020

Abstract
Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) causing Bloodstream infection (BSI) are associated with high rates of mortality . Nevertheless, only a few studies regarding the epidemiology of CRKP BSI in south China. The purpose of this study was to describe the epidemiology, clinical characteristics, and the mortality of risk factors associated with CRKP causing bloodstream infection. Methods: A retrospective study of patients with CRKP BSI was recruited from teaching hospital in south China from January 2016 to December 2018. Clinical data were collected from medical records. Results: In total, 90 patients with CRKP BSI were enrolled in the study, while 57% (51/90) of the CRKP BSI were obtained from ICU. Most CRKP BSIs originated from hospitals (81; 85%), while the rest (9; 10%) were healthcare-associated . In univariate analysis, gastrointestinal hemorrhage (p=0.029), Pitt bacteremia score (P=0.045), Charlson comorbidity index (p=0.018) and Corticosteroids use (p=0.036) and Septic shock (p=0.001) were associated with the risk factors for mortality. In a multivariate analysis, septic shock (adjusted odds ratio [aOR] 5.591, 95% confidence interval [CI] 1.405-22.246, P=0.015) and Corticosteroids use (aOR 4.148, 95% CI 1.331-12.928, P=0.014) were independently predictors of mortality.Conclusion: Our data showed that the morbidity and mortality of CRKP BSIs patient from ICU and non-ICU was no significant difference. Standardizing operation and improving nurse quality may play an important role in CRKP BSI patient in intensive care unit. Septic shock and Corticosteroids use were the independent factors of CRKP BSI patient mortality. However, the study did not show an association between invasive procedures and the development of CRKP BSI.
Study of laboratory staff' knowledge of biobanking in cote d'ivoire
Authors: Kintossou Ambroise Kouamé , N'Dri Mathias Kouamé , Money Marcelle , Cisse Souleymane , Doumbia Simini , Man-Koumba Soumahoro , Coulibaly Amadou Founzégué , Djaman Joseph Allico , Dosso Mireille 
Published: January 2020

Abstract
Backgound The biobank is a structure that manages and makes available biological resources. The biobank has medical, scientific and economic interests in an ethical manner. In 2009, Côte d'Ivoire established a biobank, which has housed the regional biobank of ECOWAS countries since 2018. To ensure optimal and efficient use of this biobank, scientific stakeholders must be aware of its existence and role. It was therefore necessary to assess the knowledge of laboratory staff on the role and activities of a biobank. Methods This is a descriptive study conducted with staff of human, animal and plant health laboratories located in southern Côte d'Ivoire. Results A total of 205 people agreed to complete the proposed questionnaire. Of these, 34.63% are biologists, 7.32% engineers, 48.78% technicians and 9.27% phD students. The average professional experience was 10.11 ± 7.83 years. Respectively, 47.32% and 43.41% have never heard of biocollection and biobank. Only 48.78% of participants had a good understanding of the role of a biobank. Technicians and phD students were less educated on the notion of biobank (p<0.000001). Biologists, although they were more educated on this issue, 21.13% of them had a misconception of biobank. Good knowledge of the role of a biobank was not significantly related to the duration of professional experience (p>0.88). Conclusion The level of knowledge of laboratory staff about biobanking remains to be improved. Training on the role, activities and interests of the biobank is important.
Structural Plasticity of EAK-16 Peptide Inducing Vesicle Membrane Leakage
Authors: Majid Abdul , Naz Farah , Khaskheli Muhammad Hassan 
Published: January 2020

Background:
Ionic complementary peptide EAK-16 has been studies for anticancer drug
delivery application. This is a 16 residues, short sequence peptide has ability to trosnform into
micro/nanoparticle via self-assembly. However, it is still not clear that how this can bind with cell
membrane to induce membrane leakage or delivering their cargo inside cell membrane.

Objective:
The main objective of this work was to understand behaviour of secondary structure
conformation of peptide in solution and at lipid membrane interfaces and membrane permeability
of synthetic ionic complementary peptide EAK-16. The corresponding secondary structure
conformation was evaluated.

Methods:
We performed biophysical investigation to probe the interaction of synthesised ionic
complementary peptide (EAK-16) with dimyristoylphospholcholine (DMPC) and
dimyristoylphosphoserine (DMPS) membrane interfaces. The folding behaviours of EAK-16 were
studied with Circular Dichroism (CD) spectroscopy. Membrane leakage with peptide was
confirmed with calcein leakage assay.

Results:
Our finding of this study showed that in aqueous phase EAK-16 was predominantly folded
into β-sheets. The temperature could alter the β-sheets. However, in DMPC and DMPS membrane
interfaces, EAK-16 adopted helical conformation. EAK-16 has preference in perturbing anionic
compared Zwitterionic lipid vesicles. This study proposed that hydrophobic grooves of EAK-16
might be a key in the association with lipid bilayers. Secondly, a charge distribution of ionic
residues would also support the orientation at lipid bilayers. This peptide membrane association
would facilitate the membrane destabilisation.

Conclusion:
This study demonstrated the supporting evidence that EAK-16 could interact with
lipid membranes and conforming to helical structure, while the helical conformation induced the
lipid membrane leakage. Overall, this study provides a physical rationale that ionic complementary
peptide can be a useful tool for designing and development of novel antibiotics and anticancer
agents along its previous drug delivery applications.
Sequence-based Identification of Arginine Amidation Sites in Proteins Using Deep Representations of Proteins and PseAAC
Authors: Naseer Sheraz , Hussain Waqar , Khan Yaser Daanial , Rasool Nouman 
Published: January 2020

Background:
Among all the major post-translational modifications, amidation seems to
be a small change, where a peptide ends with an amide group (-NH 2), not a carboxyl group
(-COOH). Thus, to study their physicochemical properties, identification of the amidation
mechanism is very important. However, the in vitro, ex vivo and in vivo identification can be
laborious, time-taking and costly. There is a dire need for an efficient and accurate computational
model to help researchers and biologists identifying these sites, in an easy manner.

Objectives:
Herein, we propose a novel predictor for the identification of arginine amide (R-Amide)
sites in proteins, by integrating the Chou’s Pseudo Amino Acid Composition (PseAAC) with deep
features. Methods: We use well-known DNNs for both the tasks of learning a feature representation
of peptide sequences and performing classifications.

Methods:
We use well-known DNNs for both the tasks of learning a feature representation of peptide sequences and performing classifications.

Results:
Among different DNNs, CNN showed the highest scores in terms of accuracy, and all other
computed measures outperformed all the previously reported predictors.

Conclusions:
Based on these results, it is concluded that the proposed model can help identify
arginine amidation in a very efficient and accurate manner, which can help scientists understand the
mechanism of this modification in proteins.
Independent Inverse System Decoupling Control Strategy of Bearingless Induction Motor
Authors: Bu Wenshao , Lu Panchao , Lu Chunxiao , Pu Yi 
Published: January 2020

Background:
In the existing inverse system decoupling methods of bearingless induction
motor, the inverse system model is more complex, and it is not easy to realize the independent control
of the magnetic suspension system. In this paper, in order to simplify its inverse system model,
an independent inverse system decoupling control strategy is proposed.

Methods:
Under the conditions of considering the current dynamics of torque windings, the state equations
of torque system and those of magnetic suspension system are established, and the independent inverse system
model of torque system and that of the magnetic suspension system are deduced. The air gap fluxlinkage
of the torque system that is needed in the magnetic suspension system is identified by an independent
voltage model. After the independent inverse model of torque system and that of magnetic suspension system
are connected in parallel, they are connected in front of the original system of a bearingless induction
motor. After this, the torque system is decoupled into two second-order integral subsystems, i.e. a fluxlinkage
subsystem and a motor speed subsystem, while the magnetic suspension system is decoupled into
another two second-order integral subsystems, i.e. the α- and β-displacement component subsystems. The
design of the additional closed-loop controller is achieved through the pole assignment method.

Result:
The obtained inverse model of the magnetic suspension system is independent of the fluxlinkage
orientation mode of torque system, and thus the flexibility of the independent control for the
torque system and magnetic suspension system is increased. The simulation results have shown that
the system has good static- and dynamic-decoupling control performance.

Conclusion:
The proposed independent inverse system decoupling control strategy is effective and
feasible.
Mobile Phone–Based Behavioral Interventions in Pregnancy to Promote Maternal and Fetal Health in High-Income Countries: Systematic Review
Authors: Hussain Tasmeen , Smith Patricia , Yee Lynn M 
Published: January 2020

Background
Chronic diseases have recently had an increasing effect on maternal-fetal health, especially in high-income countries. However, there remains a lack of discussion regarding health management with technological approaches, including mobile health (mHealth) interventions.

Objective
This study aimed to systematically evaluate mHealth interventions used in pregnancy in high-income countries and their effects on maternal health behaviors and maternal-fetal health outcomes.

Methods
This systematic review identified studies published between January 1, 2000, and November 30, 2018, in MEDLINE via PubMed, Cochrane Library, EMBASE, CINAHL, PsycINFO, Web of Science, and gray literature. Studies were eligible for inclusion if they included only pregnant women in high-income countries and evaluated stand-alone mobile phone interventions intended to promote healthy maternal beliefs, behaviors, and/or maternal-fetal health outcomes. Two researchers independently reviewed and categorized aspects of full-text articles, including source, study design, intervention and control, duration, participant age, attrition rate, main outcomes, and risk of bias. Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were followed, and the study was registered in PROSPERO before initiation.

Results
Of the 2225 records examined, 28 studies were included and categorized into 4 themes: (1) gestational weight gain, obesity and physical activity (n=9); (2) smoking cessation (n=9); (3) influenza vaccination (n=2); and (4) general prenatal health, preventive strategies, and miscellaneous topics (n=8). Reported sample sizes ranged from 16 to 5243 with a median of 91. Most studies were performed in the United States (18/28, 64%) and were randomized controlled trials (21/28, 75%). All participants in the included studies were pregnant at the time of study initiation. Overall, 14% (4/28) of studies showed association between intervention use and improved health outcomes; all 4 studies focused on healthy gestational weight. Among those, 3 studies showed intervention use was associated with less overall gestational weight gain. These 3 studies involved interventions with text messaging or an app in combination with another communication strategy (Facebook or email). Regarding smoking cessation, influenza vaccination, and miscellaneous topics, there was some evidence of positive effects on health behaviors and beliefs, but very limited correlation with improved health outcomes. Data and interventions were heterogeneous, precluding a meta-analysis.

Conclusions
In high-income countries, utilization of mobile phone–based health behavior interventions in pregnancy demonstrates some correlation with positive beliefs, behaviors, and health outcomes. More effective interventions are multimodal in terms of features and tend to focus on healthy gestational weight gain.