bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Epstein-Barr Virus Indirect Immunofluorescence Assay Results
Authors: Gorgun Selim , Havuz Seda Gudul , Mehel Dursun M. 
Published: May 2020

Abstract
Background: The aim of this study was to diagnose Epstein-Barr virüs (EBV) infection using indirect immunofluorescence assay (IIFA) method and to evaluate these patients clinically. More than 90% of children are infected with EBV until the age of six. Methods: The tests were studied by using EBV IIFA method. A total of 247 patients, 186 adults, and 61 children, were included in the study. Results: 5 (2.7%) of the adults were EBV-Capsid antigen (EBV-CA) IgM positive, 175 (94.1%) were IgG positive and 6 (3.2%) were seronegative. 10 (16.4%) of the child patients were IgM positive, which is considered as an acute infectious mononucleosis (IM) infection marker, whereas the child patients had a significantly higher IgM rate than adults (p <0.001). 39 (63.9%) of the child patients were IgG positive and 12 (19.7%) of them were seronegative. The rate of IgG positivity in children was significantly lower than in adults (p <0.001).There was no significant difference between the genders in terms of IgM and IgG positivity rates in both adults and children(p> 0.05 for each).Conclusion: These results suggest that the presence of an acute EBV infection should be considered when the patient has viral diseases with similar clinical picture especially for children.
Molecular mechanism of luteolin against inflammation based on integration of network pharmacology, transcriptomics and proteomics
Authors: Huang XiuFang , Zhang Jia-Lin , Huang Ai-Si , Huang Dan-Ping , Huang Hui-Ting , Liu Qiong , Liu Xiao-Hong , Liao Hui-Li 
Published: May 2020

Abstract
Background: Luteolin (3', 4', 5,7-tetrahydroxyflavone), a natural flavonoid exists in various medicinal plants, has strong anti-inflammatory effect. However, anti-inflammatory mechanism of luteolin has not been fully explored. Hence, we aimed to systematically investigate druggability and anti-inflammatory mechanism of luteolin based on network pharmacology. Methods: The absorption, distribution, metabolism, excretion, and toxicity of luteolin were evaluated by TCMSP server. Targets associated with luteolin and inflammation were collected from public databases, and the overlapping targets between luteolin and inflammation were analyzed by Draw Venn diagram. Then the protein-protein interaction network of luteolin against inflammation was constructed to get core genes. Further, gene function and pathway enrichment analysis were performed. Finally, in vitro experiment was carried out to estimate the accuracy of predicted target genes. Results: ADME results indicated that luteolin has great potential to be developed into a drug. 226 overlapping targets (targets of luteolin against inflammation) were screened by matching 280 targets of luteolin with 9015 targets of inflammation. 9 core targets of luteolin against inflammation were identified, including MMP9, MAPK1, HSP90AA1, CASP3, ALB, EGFR, SRC, HRAS and ESR1. Gene function were mainly involved in metabolism, energy pathways and signal transduction. Pathway enrichment results suggested that metabolic pathways, pathways in cancer, PI3K-AKT signaling pathway, Ras signaling pathway and so on might be the critical pathways of luteolin against inflammation. RT-qPCR and ELISA results indicated that luteolin decreased the expression of most of core genes at protein and mRNA levels (MMP9, MAPK1, HSP90AA1, EGFR, SRC and HRAS). Conclusions: The anti-inflammatory mechanism of luteolin were systematically investigated based on network pharmacology, RT-qPCR and ELISA. Luteolin is expounded to have great potential to be developed into a drug and target various genes and pathways to perform systematic anti-inflammatory effect.
Health-Related Quality of Life following Minimally Invasive Totally Thoracoscopic Mitral Valve Surgery
Authors: Huang Ling-chen , Chen Dao-zhong , Chen Liang-wan , Xu Qi-chen , Zheng Zi-he , DAI XIAOFU 
Published: May 2020

Abstract
Background
In order to compare the impact of two different approaches to mitral valve surgery on health-related quality of life, we conducted a retrospective study comparing minimally invasive totally thoracoscopic mitral valve surgery with median sternotomy mitral valve surgery.
Methods
A total of 163 patients who underwent mitral valve surgery at our institution between January 1, 2019 and December 31, 2019 were enrolled. In 163 patients, mitral valve surgery was performed using either totally thoracoscopic approach (TA n = 78) or median sternotomy approach (SA n = 85). We used the Scar Cosmesis Assessment and Rating Scale and the Numerical Rating Scale to measure the pain intensity and the aesthetic appearance of surgical incision and used the MOS SF-36 to assess the HRQoL.
Results
The two groups of patients were similar in terms of demographic, echocardiography data and postoperative complications. The pain intensity and aesthetic appearance of the totally thoracoscopic approach were significantly better than that of the median sternotomy approach. Significant differences in the subscale of the SF-36 were found between the two groups.
Conclusions
When compared to the median sternotomy mitral valve surgery, totally thoracoscopic mitral valve surgery has an equally good outcome, while improving aesthetic appearance and reducing pain intensity. Our study suggested that totally thoracoscopic approach is superior to median sternotomy approach in terms of pain intensity, aesthetic appearance and HRQoL.
Causes of acute respiratory hospitalizations predict survival in fibrosing interstitial lung diseases
Authors: Salonen Johanna , Vähänikkilä Hannu , Purokivi Minna , Kaarteenaho Riitta 
Published: May 2020

Abstract
Background
Acute exacerbation of ILD (AE-ILD) is a common reason for hospitalization; it is also associated with significant mortality. Less is known about the prognostic significance of other events causing acute, non-elective hospitalizations in ILD patients.
Methods
ILD patients hospitalized due to acute respiratory worsening were collected from medical records. Reasons for respiratory deterioration were classified into AE-ILDs and other causes. Clinical features and survival data of idiopathic pulmonary fibrosis (IPF) and other types of ILDs were evaluated and compared.
Results
In all, 237 patients (138 with IPF and 99 with other ILD) fulfilled the inclusion criteria. Of the non-IPF ILD types, the most prevalent subgroups were connective tissue disease-associated ILD (n = 33) and asbestosis (n = 22). The most common cause for hospitalization was AE-ILD explaining 41% of hospitalizations. Lower respiratory tract infection (22%), subacute progression of ILD (12%) and cardiovascular causes (7.2%) were other common reasons for hospital treatment. Patients with a lower respiratory tract infection had a more favorable prognosis compared with patients with AE-ILD. AE-ILDs were less fatal than cardiovascular or concurrent non-ILD-related causes for hospitalizations in non-IPF patients. High Gender-Age-Physiology (GAP) index was a marker for shortened survival and earlier AE-ILDs in all patients. IPF patients had a significantly shorter overall and post-hospitalization survival time compared with other ILDs.
Conclusions
Most respiratory hospitalizations in ILD patients were related to causes other than AE-ILD, which highlights the importance of accurate differential diagnosis in order to target the appropriate treatment for each ILD patient.
Imbalance of Laminar-specific Excitatory and Inhibitory Circuits of the Orbito frontal Cortex in Autism
Authors: Liu Xuefeng , Bautista Julied , Liu Edward , Zikopoulos Basilis 
Published: May 2020

Abstract
Background:The human orbitofrontal cortex (OFC) is involved in assessing the emotional significance of events and stimuli, emotion-based learning, allocation of attentional resources, and social cognition. Little is known about the structure, connectivity and excitatory/inhibitory circuit interactions underlying these diverse functions in human OFC, as well as how the circuit is disrupted in individuals with autism spectrum disorder (ASD). Methods: We used post-mortem brain tissue from neurotypical adults and individuals with ASD. We examined the morphology and distribution of myelinated axons across cortical layers in OFC, at the single axon level, as a proxy of excitatory pathways. In the same regions, we also examined the laminar distribution of all neurons and neurochemically- and functionally-distinct inhibitory neurons that express the calcium-binding proteins parvalbumin (PV), calbindin (CB), and calretinin (CR). Results:We found that the density of myelinated axons increased consistently towards layer 6, while the average axon diameter did not change significantly across layers in both groups. However, both the density and diameter of myelinated axons were significantlylower in the ASD group compared with the Control group. The distribution pattern and density of the three major types of inhibitory neurons was comparable between groups, but there was a significant reduction in the density of excitatory neurons across OFC layers in ASD. Limitations: This study is limited by the availability of human post-mortem tissueoptimally processed for high-resolution microscopy and immunolabeling, especially from individuals with ASD. Conclusions:The balance between excitation and inhibition in OFC is at the core of its function, assessing and integrating emotional and social cues with internal states and external inputs. Our preliminary results provide evidence for laminar-specific changes in the ratio of excitation/inhibition in OFC of adults with ASD, with an overall weakening and likely disorganization of excitatory signals and a relative strengthening of local inhibition. These changes likely underlie pathology of majorOFC communications with limbic or other cortices and the amygdala in individuals with ASD, and may provide the anatomic basis for disrupted transmission of signals for social interactions and emotions in autism.
Socioeconomic status inequity in inpatient service utilization based on need among migrants: Evidence from a national study in China
Authors: Wang Yi , Jing Zhengyue , Ding Lulu , Tang Xue , Feng Yuejing , Li Jie , Chen Zhuo , Zhou Chengchao 
Published: May 2020

Abstract
Background: Equity in access to healthcare is a major health policy challenge in many low- and middle- income countries. However, millions of people, especially migrants, do not have the adequate access to health care they need. This study aims to identify the socioeconomic status (SES) inequities in inpatient service utilization based on need among migrants by using a nationally representative study in China.Methods: The data used in this study was derived from the 2014 National Internal Migrant Population Dynamic Monitoring Survey collected by the National Health Commission of China. We used logistic regression method and Blinder-Oaxaca decomposition and calculated the concentration index to measure inequities of SES in inpatient service utilization based on need. Sample weights provided in the survey were applied in all the analysis to represent the China population.Results: The total number of the migrants who needed inpatient service told by doctors was 7592, of which, 1667 (21.96%) did not use the inpatient services (unmet inpatient service need). Results showed that inpatient service utilization concentrated among high-SES migrants (Concentration Index: 0.041, p <0.001) and the decomposition results suggested that about 53.76% of the total SES gap in inpatient service utilization could be attributed to the gradient effect. After adjusting for other confounding variables, the odds ratios of inpatient service utilization by internal migrants with high SES according to educational attainment, economic status, and employment status were 1.41 (95% CI 1.08-1.85, p =0.012), 1.25 (95% CI 1.01-1.56, p =0.046), and 1.62 (95% CI 1.12-2.36, p =0.011), respectively.Conclusion: This study observed an inequity in inpatient service utilization where the utilization concentrates among high SES migrants. This suggests that future policies should make the reimbursement more pro-poor among migrants in primary care and use more effective policies targeting the migrants with low educational attainment and unemployed, such as health education activities.
The efficacy and safety of compound glycyrrhizin combined with desloratadine in the treatment of chronic urticaria: protocol for a systematic review of randomized controlled trials
Authors: Wang Qiuyue , Hu Xingxin , Li Mao , Luo Qian , Hao Pingsheng 
Published: May 2020

Abstract
Background: Chronic urticaria (CU) is a common skin disease characterized by a short-term (<24 hours) spontaneous skin rash (urticaria) with or without angioedema that lasts longer than 6 weeks. CU is not life-threatening but has been shown to have a significant impact on the physical and mental health of patients. Because of chronic itching or physical discomfort in patients with CU, symptoms such as the repeated occurrence of red, swollen, itchy, anxiety, insomnia, and psychological stress are prone to occur. Whereas, there is no related systematic review and meta-analysis. Thus, this systematic review protocol aims to describe a systematic review and meta-analysis to testify the efficacy and safety of compound glycyrrhizin (CG) combined with desloratadine in the treatment of CU.Methods: Our systematic review will search all randomized controlled trials (RCTs) for CG combined with desloratadine in the treatment of CU, electronically and manually, regardless of publication status and language, until January 14, 2020. Databases include PubMed, EMBASE, Web of Science, Cochrane Controlled Trials Register (CENTRAL), China National Knowledge Infrastructure (CNKI), China Biomedical Literature Database (CBM), Chinese Science Journal Database (VIP Database) and Wanfang database. Other sources, including reference lists of identified publications and meeting minutes, will also be searched. Manually search for grey literature, including unpublished conference articles. The main outcomes contain the total effective rate, the urticaria activity score (UAS), Itching score, the chronic urticaria quality of life questionnaire (CU-Q2oL), or other validated symptom scores and the effective rate and adverse events from baseline to the end of studies. This study will provide a comprehensive review of the available evidence for the treatment of CU with this therapy. We will assess the risk of bias with the Cochrane Risk of Bias Tool, narratively synthesize the extracted data and conduct a meta-analysis of studies with similar characteristics. Two independent raters will screen articles and assess the risk of bias.Discussion: This study will provide a high-quality synthesis of the effects of compound glycyrrhizin (CG) combined with desloratadine in the treatment of CU. We hope that this review will give more convincing proof to assist clinicians during the decision-making process when dealing with CU.Systematic review registration: PROSPERO CRD42020165478
The analysis of Upper tract Urinary Stone Composition in HIV-positive Patients: a single center experience
Authors: Menghua Wu , Xin Zheng , Bohan Fan , Yu Zhang , Jimao Zhao 
Published: May 2020

Abstract
Background: To appraise the most common stone composition, serum and urinary biochemical features in HIV-positive patients with urolithiasis.Methods: We retrospectively searched the records of HIV-positive patients in our stone registry. A retrospective study was generated from our registry. Collecting statistics about fundamental indexes and analyzing them is our basic procedure. Medications were considered. Groups were compared using the Student t and chi-square/Fisher exact tests with significance considered at p<0.05.Results: In total of 101 patients were included age, gender, Body Mass Index(BMI), serum calcium, serum uric acid(UA), stone position, and operative method were statistically described. The serum UA of HIV-positive group was higher than that of the negative group (P = 0.028). Patients with HIV-infected had a higher incidence of calcium oxalate dehydrate (CaODH) (p<0.001). The evaluation of overall stone composition showed that the number of CaODH was higher in HIV-positive group (21.4% vs 8.5%, p < 0.001). There was no significant difference in single calcium oxalate monohydrate(CaOMH) (p = 0.146), and UA (p = 0.7) between the two groups.Conclusions: In our study the highest stone proportion in HIV patients is calcium oxalate (CaO). HIV positive patients should pay attention to the diet, especially the food containing more calcium. In HIV-positive patients, the level of serum UA should be controlled accordingly. In the development of highly active antiretroviral therapy (HARRT), for low-risk patients, it may not be necessary to consider too much the factors of protease inhibitors (PIs) and other drugs forming urinary calculus.
Development and Validation of a Novel Glycolysis-Related Risk Signature for Predicting Survival in Pancreatic Adenocarcinoma
Authors: Li Ang , Hou Sinan , Chen Jian , Hou Huimin , Jiang Yanfang 
Published: May 2020

Abstract
Background
Pancreatic adenocarcinoma (PAAD) is one of the leading causes of cancer death worldwide. Through data mining, an increasing number of biomarkers have been identified for predicting survival of PAAD. However, the ability of single gene biomarkers to predict patient survival is still insufficient. This study aimed to develop a novel risk signature for predicting survival of PAAD.
Methods
mRNA expression profiling was performed in a large PAAD cohort (N = 177) from The Cancer Genome Atlas (TCGA) database. Gene Set Enrichment Analysis (GSEA) was analyzed to detect whether the gene sets showed statistically differences between PAAD and adjacent normal tissues. Univariate Cox regression analysis was used to analyze and identify genes related to overall survival (OS), then subjected to multivariable Cox regression to further confirm the prognostic genes and obtain the coefficients. The expression level of selected genes weighted by their coefficients through linearly combining, we constructed a risk score formula for prognostic prediction. The three-mRNA signature for survival prediction is validated by Kaplan–Meier curve analysis.
Results
We demonstrated that a set of three genes (KIF20A, CHST2, and MET) were significantly associated with OS. Based on this three-gene signature, 177 PAAD patients were classified into high-risk groups and low-risk groups using the median risk score as cut off value. Additionally, multivariate Cox regression analysis revealed that the three-gene signature had independent prognostic value.
Conclusions
To our best knowledge, we first develop a glycolysis-related risk signature for predicting survival of pancreatic adenocarcinoma. The findings provide insight into identification of patients with poor prognosis in PAAD and improve novel therapy targets for this disease.
Matched Analysis of Postoperative Pancreatic Fistula Using Fistula Risk Score According to Pancreaticoenterostomy Methods
Authors: Lee Seungmin , Paik Kwang Yeol , Oh Ji Seon , Kim Eung Kook 
Published: May 2020

Abstract
Background

To examine whether pancreaticogastrostomy (PG) or pancreaticojejunostomy (PJ) is the better reconstructive method to reduce Postoperative Pancreatic Fistula (POPF), after pancreaticoduodenectomy (PD) according to the fistula risk.

Methods

An institutional database was reviewed for patients undergoing PD between January 2008 and August 2019. 159 patients were stratified into four groups according to the Clinical Risk Score-Pancreatic Fistula (CRS-PF). POPF according to 4 risk groups was compared between PJ and PG.

Results

Of the 159 patients, 82 underwent PG (51.6%) and 77 underwent PJ (48.4%) reconstruction. POPF rate was 15.1% and 17.1% (n=14) in the PG group and 12.9% (n=10) in the PJ group (P =0.51). POPF rates were not different in intermediate, low and negligible risks between two reconstructive methods. In High risk group (n=47), there was 4 (22.2%) POPF in PJ group and 9 (31.0%) in PG group, respectively (p=0.74).

Conclusions

In PD, there was no superior method of reconstruction with regards of POPF, even in high risk glands.
D-mannose ameliorates autoimmune phenotypes in mouse models of lupus
Authors: Wang Haiting , Teng Xiangyu , Abboud Georges , Li Wei , Ye Shuang , Morel Laurence 
Published: May 2020

Abstract
Background
Systemic lupus erythematosus is a disorder of immune regulation characterized by overproduction of autoantibodies. D-mannose is a C-2 epimer of glucose that exhibits immunoregulatory effects in models of autoimmune diseases, such as type 1 diabetes, induced rheumatoid arthritis, and airway inflammation. This study was conducted to evaluate the efficacy of D-mannose treatment in mouse models of lupus.
Methods
The effect of D-Mannose was evaluated by flow cytometry on the in vitro activation of C57BL/6 (B6) murine bone marrow derived dendritic cells and their ability to induce antigen specific CD4+ T cell proliferation and activation. The effect of D-mannose administration in vivo on the frequency of Foxp3+ regulatory T cells in B6 mice was assessed by flow cytometry. D-mannose was administered to two models of lupus: the chronic graft-versus-host disease (cGVHD) induced model and the B6.lpr spontaneous model. Autoantibody production was measured by ELISA and immune activation by flow cytometry. Results were compared by two-tailed statistics: unpaired or paired t tests, or Mann-Whitney U tests depending on whether the data was normally distributed.
Results
D-mannose inhibited the maturation of bone marrow dendritic cells and their induction of antigen-specific T cell proliferation and activation in vitro. In vivo, D-mannose increased the frequency of Foxp3+ regulatory T cells in unmanipulated control mice. In the cGVHD model of induced lupus, D-mannose treatment decreased autoantibody production, with a concomitant reduction of the frequency of effector memory and follicular helper T cells as well as germinal center B cells and plasma cells. These results were partially validated in the B6.lpr model of spontaneous lupus.
Conclusion
Overall, our results suggest that D-mannose ameliorates autoimmune activation in models of lupus, at least partially due to its expansion of Treg cells, the induction of immature conventional dendritic cells and the downregulation of effector T cells activation. D-Mannose showed however a weaker immunomodulatory effect in lupus than in other autoimmune diseases.