bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic capitalism and stabilizing selection of antimicrobial resistance genotypes in Escherichia coli
Authors: Ford Colby T. , Zenarosa Gabriel Lopez , Smith Kevin B. , Brown David C. , Williams John , Janies Daniel 
Published: June 2020

AbstractAntimicrobial resistance (AMR) in pathogenic strains of bacteria, such as Escherichia coli (E. coli), adversely impacts personal and public health. In this study, we examine competing hypotheses for the evolution of AMR including (i) ‘genetic capitalism’ in which genotypes that confer antibiotic resistance are gained and not often lost in lineages, and (ii) ‘stabilizing selection’ in which genotypes that confer antibiotic resistance are gained and lost often. To test these hypotheses, we assembled a dataset that includes annotations for 409 AMR genotypes and a phylogenetic tree based on genome‐wide single nucleotide polymorphisms from 29 255 isolates of E. coli collected over the past 134 years. We used phylogenetic methods to count the times each AMR genotype was gained and lost across the tree and used model‐based clustering of the genotypes with respect to their gain and loss rates. We demonstrate that many genotypes cluster to support the hypothesis for genetic capitalism while a few genotypes cluster to support the hypothesis for stabilizing selection. Comparing the sets of genotypes that fall under each of the hypotheses, we found a statistically significant difference in the breakdown of resistance mechanisms through which the AMR genotypes function. The result that many AMR genotypes cluster under genetic capitalism reflects that strong positive selective forces, primarily induced by human industrialization of antibiotics, outweigh the potential fitness costs to the bacterial lineages for carrying the AMR genotypes. We expect genetic capitalism to further drive bacterial lineages to resist antibiotics. We find that antibiotics that function via replacement and efflux tend to behave under stabilizing selection and thus may be valuable in an antibiotic cycling strategy.
High‐efficient generation of VCAM‐1+ mesenchymal stem cells with multidimensional superiorities in signatures and efficacy on aplastic anaemia mice
Authors: Wei Yimeng , Zhang Leisheng , Chi Ying , Ren Xiang , Gao Yuchen , Song Baoquan , Li Chengwen , Han Zhibo , Zhang Lei , Han Zhongchao 
Published: June 2020

AbstractObjectiveLongitudinal studies have indicated VCAM‐1+ mesenchymal stem/stromal cells (MSCs) as promising resources in regenerative medicine, yet the abundance in gene expression is far from adequate in the advantaged and “discarded” hUC‐MSCs. Thus, high‐efficient preparation and systematic dissection of the signatures and biofunctions of the subpopulation is the prerequisite for large‐scale clinical applications.Materials and methodsWe primarily took advantage of a cytokine‐based programming strategy for large‐scale VCAM‐1+ hUC‐MSC generation (III‐MSCs). Thereafter, we conducted multifaceted analyses including cytomorphology, immunophenotype, cell vitality, multilineage differentiation, whole‐genome analysis, tube formation and Matrigel plug assay, lymphocyte activation and differentiation, and systemic transplantation for aplastic anaemia (AA) treatment.ResultsIII‐MSCs with high‐proportioned VCAM‐1 expression were obtained by combining IL‐1β, IL‐4 with IFN‐γ, which exhibited comparable immunophenotype with untreated hUC‐MSCs (NT‐MSCs) but revealed multidimensional superiorities both at the cellular and molecular levels. Simultaneously, systemic infusion of III‐MSCs could significantly ameliorate clinicopathological features and finally help facilitate haematopoietic reconstruction and immunoregulation in AA mice.ConclusionsWe have established a high‐efficient procedure for large‐scale generation of III‐MSCs with preferable signatures and efficacy upon aplastic anaemia in mice. Our findings suggested that III‐MSCs were advantageous sources with multifaceted characteristics for regenerative medicine.
Changes in capture rates and body size among vertebrate species occupying an insular urban habitat reserve
Authors: Stanley Thomas R. , Clark Rulon W. , Fisher Robert N. , Rochester Carlton J. , Root Stephanie A. , Lombardo Keith J. , Ostermann‐Kelm Stacey D. 
Published: June 2020

AbstractLong‐term ecological monitoring provides valuable and objective scientific information to inform management and decision‐making. In this article, we analyze 22 years of herpetofauna monitoring data from the Point Loma Ecological Conservation Area (PLECA), an insular urban reserve near San Diego, CA. Our analysis showed that counts of individuals for one of the four most common terrestrial vertebrates declined, whereas counts for other common species increased or remained stable. Two species exhibited declines in adult body length, whereas biomass pooled over the five most common species increased over time and was associated with higher wet season precipitation. Although the habitat and vegetation at PLECA have remained protected and intact, we suspect that changes in arthropod communities may be driving changes in the abundance, growth, and development of insectivorous lizards. This study underscores the value of long‐term monitoring for establishing quantitative baselines to assess biological changes that would otherwise go undetected.
A thousand pleasures are not worth a single pain: The compensation argument for Schopenhauer's pessimism
Authors: Simmons Byron 
Published: June 2020

AbstractPessimism is, roughly, the view that life is not worth living. In chapter 46 of the second volume of The World as Will and Representation, Arthur Schopenhauer provides an oft‐neglected argument for this view. The argument is that a life is worth living only if it does not contain any uncompensated evils; but since all our lives happen to contain such evils, none of them are worth living. The now standard interpretation of this argument (endorsed by Kuno Fischer and Christopher Janaway) proceeds from the claim that the value—or rather valuelessness—of life's goods makes compensation impossible. But this interpretation is neither philosophically attractive nor faithful to the text. In this article, I develop and defend an alternative interpretation (suggested by Wilhelm Windelband and Mark Migotti) according to which it is instead the actual temporal arrangement of life's goods and evils that makes compensation impossible.
Functional and structural characterization of PII‐like protein CutA does not support involvement in heavy metal tolerance and hints at a small‐molecule carrying/signaling role
Authors: Selim Khaled A. , Tremiño Lorena , Marco‐Marín Clara , Alva Vikram , Espinosa Javier , Contreras Asunción , Hartmann Marcus D. , Forchhammer Karl , Rubio Vicente 
Published: June 2020

The PII‐like protein CutA is annotated as being involved in Cu2+ tolerance, based on analysis of Escherichia coli mutants. However, the precise cellular function of CutA remains unclear. Our bioinformatic analysis reveals that CutA proteins are universally distributed across all domains of life. Based on sequence‐based clustering, we chose representative cyanobacterial CutA proteins for physiological, biochemical, and structural characterization and examined their involvement in heavy metal tolerance, by generating CutA mutants in filamentous Nostoc sp. and in unicellular Synechococcus elongatus. However, we were unable to find any involvement of cyanobacterial CutA in metal tolerance under various conditions. This prompted us to re‐examine experimentally the role of CutA in protecting E. coli from Cu2+. Since we found no effect on copper tolerance, we conclude that CutA plays a different role that is not involved in metal protection. We resolved high‐resolution CutA structures from Nostoc and S. elongatus. Similarly to their counterpart from E. coli and to canonical PII proteins, cyanobacterial CutA proteins are trimeric in solution and in crystal structure; however, no binding affinity for small signaling molecules or for Cu2+ could be detected. The clefts between the CutA subunits, corresponding to the binding pockets of PII proteins, are formed by conserved aromatic and charged residues, suggesting a conserved binding/signaling function for CutA. In fact, we find binding of organic Bis‐Tris/MES molecules in CutA crystal structures, revealing a strong tendency of these pockets to accommodate cargo. This highlights the need to search for the potential physiological ligands and for their signaling functions upon binding to CutA.DatabasesStructural data are available in Protein Data Bank (PDB) under the accession numbers 6GDU, 6GDV, 6GDW, 6GDX, 6T76, and 6T7E.
Wild bee declines linked to plant‐pollinator network changes and plant species introductions
Authors: Mathiasson Minna E. , Rehan Sandra M. 
Published: June 2020

Abstract
The mutualistic interactions of plant‐pollinator networks provide myriad economic, ecological, and cultural constituents without which there would be severe environmental and societal consequences. Plant‐pollinator networks are becoming increasingly vulnerable to disturbance through intensifying anthropogenic land use and climate change.
Wild bees are central to pollination and documenting unique regional interactions between wild bees and floral hosts provides powerful insights into local ecology and biodiversity in addition to the potential to detect temporal network variation.
This study characterises the changes in a northern New England wild bee plant‐pollinator network over the past 125 years and reveals a striking increase in exotic bee and plant taxa over time. Here we document that declining wild bee species have historic ties to threatened and endangered plant species. These data provide a rare insight into the fragile nature of plant‐pollinator networks.
Notable specialist interactions between native taxa that were recorded in historical networks have been lost, most likely due to local extirpation of these now threatened and endangered plant species. Subsequent monitoring and conservation efforts focused on habitat restoration for declining wild bee and plant taxa are fundamental to the future preservation of regional native diversity.
Economic distress and health: A fixed effects analysis of low‐income persons in Singapore
Authors: Ng Irene Y.H. , Tan Jian Qi 
Published: June 2020

Using fixed effects regressions, this study examined the transactional effects of different types of economic status on the physical and mental health of low‐income persons in Singapore. Among the economic variables considered, unemployment and arrears had the most significant effects. This signals the physical and psychological distress of being out of work and possessing arrears while income is low. The insignificant effects of poor health on household earnings suggest possible earnings supplementation by other household members. The findings imply the fruitfulness of dually improving employment prospects and health, work activation that takes into consideration mental health effects, increasing affordability of health services in liberal welfare systems, improving mental bandwidth through reducing arrears accounts, and short lag time from application to receipt of assistance. These implications point to a larger question on health and welfare systems in an increasingly uncertain economic environment for households in poverty.Key Practitioner Message: • Employment and health are inter‐related: employment assistance incorporating physical and mental health interventions and vice versa can be more effective than either alone; • Compared with decreasing arrears amounts, reducing the number of arrears can more effectively relieve mental distress; • Effects between economic distress and health materialise quickly; minimising the lag time between application and receipt of assistance will provide great relief.
HDAC1‐mediated deacetylation of LSD1 regulates vascular calcification by promoting autophagy in chronic renal failure
Authors: Zhou Jiajun , Zhou Han , Liu Caixin , Huang Lin , Lu Dongmei , Gao Chaoqing 
Published: June 2020

AbstractChronic renal failure (CRF) is commonly associated with various adverse consequences including pathological vascular calcification (VC), which represents a significant clinical concern. Existing literature has suggested the involvement of histone deacetylases (HDACs) in the progression of CRF‐induced VC. However, the underlying molecular mechanisms associated with HDACs remain largely unknown. Therefore, we established the adenine‐induced CRF rat model and in vitro VC models based on vascular smooth muscle cells (VSMCs) to examine HDAC1/lysine demethylase 1A (LSD1)/SESN2 as a novel molecular pathway in CRF‐induced VC. Our initial results demonstrated that HDAC1 reduced the formation of VC in vivo and in vitro. HDAC1 was found to deacetylate LSD1, which subsequently led to impaired transcriptional activity in CRF‐induced VC. Moreover, our results illustrated that LSD1 diminished the enrichment of H3K4me2 at the SESN2 promoter. Autophagy was identified as a vasculo‐protective element against calcification in VC. Finally, we found that the inhibitory effects of HDAC1 overexpression on VC were partially abolished via over‐expressed LSD1 in adenine‐induced CRF model rats and in high phosphate‐induced VSMCs. Taken together, these results highlight the crucial role of HDAC1 as an antagonistic factor in the progression of VC in CRF, and also revealed a novel regulatory mechanism by which HDAC1 operates. These findings provide significant insight and a fresh perspective into promising novel treatment strategies by up‐regulating HDAC1 in CRF.
Novel evidence for oncogenic piRNA‐823 as a promising prognostic biomarker and a potential therapeutic target in colorectal cancer
Authors: Feng Junlan , Yang Muqing , Wei Qing , Song Feifei , Zhang Youhua , Wang Xiaodong , Liu Bin , Li Jiyu 
Published: June 2020

AbstractpiRNA‐823 as a member of the piRNA family is reported to promote tumour cell proliferation in multiple myeloma and hepatocellular cancer. However, few studies on the function of piRNA‐823 in colorectal cancer (CRC). Our present study data showed that piRNA‐823 plays an oncogene role in CRC cells. Inhibition of piRNA‐823 can significantly inhibit the proliferation, invasion and apoptosis resistance of CRC cells. Mechanism studies have shown that piRNA‐823 inhibits the ubiquitination of hypoxia‐inducible factor‐1 alpha (HIF‐1α) by up‐regulating the expression of Glucose‐6‐phosphate dehydrogenase (G6PD) and ultimately up‐regulates the glucose consumption of carcinoma cells and inhibits the content of intracellular reactive oxygen species (ROS). Therefore, we speculate piRNA‐823 promotes the proliferation, invasion and apoptosis resistance of CRC cells by regulating G6PD/HIF‐1α pathway. In this study, we set up the cancer‐promoting function recovery experiment of piRNA‐823 by silencing G6PD gene to confirm the dominance of the above‐mentioned pathways. Using clinical samples, we found that overexpression of piRNA‐823 correlated with poor overall survival and predicted a poor response to adjuvant chemotherapy of patients with CRC. In a word, our research has further enriched the theory of piRNA‐823 promoting the progression of CRC, and laid a solid foundation for the development of piRNA‐823‐based gene therapy for CRC and its use as a promising prognostic biomarker in CRC patients.
Effect of zirconia decontamination protocols on bond strength and surface wettability
Authors: Noronha Mayara dos Santos , Fronza Bruna Marin , André Carolina Bosso , de Castro Eduardo Fernandes , Soto‐Montero Jorge , Price Richard Bengt , Giannini Marcelo 
Published: June 2020

AbstractObjectiveTo evaluate the effects of human saliva decontamination protocols on bond strength of resin cement to zirconia (Y‐PSZ), wettability, and microbial decontamination.Materials and methodsZirconia plates were sandblasted and divided into (a) not contaminated, (b) contaminated with human saliva and: (c) not cleaned, (d) cleaned with air‐water spray, (e) cleaned with 70% ethanol, (f) cleaned with Ivoclean, or (g) cleaned with nonthermal atmospheric plasma (NTAP). The wettability and microbial decontamination of the surfaces were determined after saliva contamination or cleaning. Monobond Plus (Ivoclar Vivadent) was applied after cleaning, followed by Variolink LC (Ivoclar Vivadent). The samples were stored 1 week before shear bond strength (SBS) testing, and data (SBS and wettability) were analyzed by one‐way analysis of variance and Tukey test (α = .05).ResultsSaliva contamination reduced SBS to zirconia compared to not contaminated. Both Ivoclean and NTAP produced higher SBS compared to not cleaned and were not significantly different from the not contaminated. Ivoclean produced the highest contact angle, and NTAP the lowest. With the exception of using just water‐spray, all cleaning protocols decontaminated the specimens.ConclusionsBoth Ivoclean and NTAP overcame the effects of saliva contamination, producing an SBS to zirconia comparable to the positive control.Clinical significanceDental ceramics should be cleaned prior to resin cementation to eliminate the effects of human saliva contamination, and Ivoclean and NTAP are considered suitable materials for this purpose.
Can the U.S. Interbank Market Be Revived?
Authors: KIM KYUNGMIN , MARTIN ANTOINE , NOSAL ED 
Published: June 2020

AbstractThe U.S. interbank market essentially disappeared as the reserve supply dramatically increased after the 2007–2008 crisis. We build a model to study whether the interbank market can revive if the reserve supply decreases sufficiently. The market may not revive due to balance sheet costs associated with recent banking regulations. Although interbank volume may initially increase as reserves decline from abundant levels, the balance sheet costs may engender changes in market structure that completely replace interbank trading by nonbank lending to banks. This nonmonotonic response could lead to misleading forecasts about future interbank volumes.
Melatonin restores neutrophil functions and prevents apoptosis amid dysfunctional glutathione redox system
Authors: NaveenKumar Somanathapura K. , Hemshekhar Mahadevappa , Jagadish Swamy , Manikanta Kurnegala , Vishalakshi Gopalapura J. , Kemparaju Kempaiah , Girish Kesturu S. 
Published: June 2020

AbstractMelatonin is a chronobiotic hormone, which can regulate human diseases like cancer, atherosclerosis, respiratory disorders, and microbial infections by regulating redox system. Melatonin exhibits innate immunomodulation by communicating with immune system and influencing neutrophils to fight infections and inflammation. However, sustaining redox homeostasis and reactive oxygen species (ROS) generation in neutrophils are critical during chemotaxis, oxidative burst, phagocytosis, and neutrophil extracellular trap (NET) formation. Therefore, endogenous antioxidant glutathione (GSH) redox cycle is highly vital in regulating neutrophil functions. Reduced intracellular GSH levels and glutathione reductase (GR) activity in the neutrophils during clinical conditions like autoimmune disorders, neurological disorders, diabetes, and microbial infections lead to dysfunctional neutrophils. Therefore, we hypothesized that redox modulators like melatonin can protect neutrophil health and functions under GSH and GR activity–deficient conditions. We demonstrate the dual role of melatonin, wherein it protects neutrophils from oxidative stress‐induced apoptosis by reducing ROS generation; in contrast, it restores neutrophil functions like phagocytosis, degranulation, and NETosis in GSH and GR activity–deficient neutrophils by regulating ROS levels both in vitro and in vivo. Melatonin mitigates LPS‐induced neutrophil dysfunctions by rejuvenating GSH redox system, specifically GR activity by acting as a parallel redox system. Our results indicate that melatonin could be a potential auxiliary therapy to treat immune dysfunction and microbial infections, including virus, under chronic disease conditions by restoring neutrophil functions. Further, melatonin could be a promising immune system booster to fight unprecedented pandemics like the current COVID‐19. However, further studies are indispensable to address the clinical usage of melatonin.
Growth, income distribution and unemployment in a two‐sector economy
Authors: Chattopadhyay Subhasankar 
Published: June 2020

AbstractWe build a two‐sector general equilibrium growth model with capital‐intensive consumption goods and a labour‐intensive investment goods sector to investigate the coexistence of growth and unemployment. The model uses heterogeneity in saving behaviour, introduces an effective demand problem, has full employment of capital with input non‐substitutability and shows that the aggregate labour employment is determined by available capital along with commodity market equilibrium. The long‐run growth may not be balanced, and under biased growth, the level of unemployment may monotonically increase or decrease over time, or may first increase (decrease) and then decrease (increase). Such possible unemployment paths help us tightly define “jobless growth”.
Dimensional Changes of Lumbar Intervertebral Foramen in Direct Anterior Approach‐Specific Hyperextension Supine Position
Authors: Liu Ming‐yang , Wang Hai‐bo , Liu Shi‐wei , Zhang Guan‐peng , Liu Jian‐guo , Yang Chen 
Published: June 2020

ObjectiveTo investigate the changes in the lumbar intervertebral foramen (LIVF) dimensions from neutral supine to direct anterior approach (DAA)‐specific hyperextension supine position through a standardized three‐dimensional (3D) reconstruction computerized tomography (CT) method.MethodsA total of 35 healthy volunteers (18 men and 17 women) were included in this retrospective study. The mean age of enrolled subjects was 28.9 ± 5.0 years. In September 2018, all the individuals underwent a 3D CT scan of the lumbar spine in neutral and 30° hyperextension supine positions, which mimicked the patient’s position in DAA total hip arthroplasty (THA). The dimensions of the LIVF, including foraminal area, height, and width, were measured on 3D reconstructed CT models at all lumbar foraminal levels. Foraminal area was defined as the area bounded by the adjacent superior and inferior vertebral pedicles, the posterosuperior boundary of the inferior vertebral body, the surface of the intervertebral disc posteriorly, the posteroinferior boundary of the superior vertebral body, and the surface of the ligamentum flavum anteriorly. Foraminal height was defined as the longest distance between the border of the superior and the inferior pedicle. Foraminal width was defined as the shortest distance between the posteroinferior edge of the superior vertebrae and the opposing boundary. Subgroup analysis and multiple linear regression were used to evaluate the relationship between percentage changes of the LIVF dimensions and side, sex, and age.ResultsThe LIVF dimensions varied significantly between the two positions at all levels (P < 0.05). From neutral to hyperextension supine position, the foraminal area reduced by 20.1% at lumbar 1–2 (L1–2), 22.6% at L2–3, 19.9% at L3–4, 18.1% at L4–5, and 12.0% at lumbar 5–sacral 1 (L5–S1) level, respectively; the foraminal height reduced by 9.5% at L1–2, 10.5% at L2–3, 9.5% at L3–4, 9.6% at L4–5, and 6.1% at L5–S1 level, respectively; the foraminal width reduced by 12.8% at L1–2, 14.5% at L2–3, 13.0% at L3–4, 10.4% at L4–5, and 8.4% at L5–S1 level, respectively. The changes in LIVF dimensions were biggest at L2–3 level and smallest at L5–S1 level. Subgroup analysis showed that there were no significant differences in the percentage changes of LIVF dimensions between the sexes and sides (P > 0.05). Multiple linear analysis showed that the percentage changes of LIVF dimensions were not related to side, sex, and age (P > 0.05).ConclusionThe dimensions of the LIVF showed significant decrease at all levels in the DAA‐specific hyperextension supine position compared with the neutral supine position, and the percentage changes of LIVF dimensions were not influenced by side, sex, and age.