bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a pan-neuronal genetic driver inAedes aegyptimosquitoes
Authors: Zhao Zhilei , Tian David , McBride Carolyn S. 
Published: August 2020

AbstractThe mosquitoAedes aegyptiis the primary worldwide vector of arboviruses that infect humans, including dengue, Zika, chikungunya, and yellow fever. Recent advances in transgenic technology have yielded important new insight into the biology of this disease vector. The early development of neurogenetic tools, in particular, is beginning to shed light on the neural basis of behaviors that allowAe. aegyptito thrive in human environments and find and bite human hosts. Despite these advances, a pan-neuronal expression driver remains elusive. Pan-neuronal drivers give researchers genetic access to all neurons and thus provide a critical entry point for circuit dissection. Here, we describe our efforts to generate pan-neuronal drivers inAe. aegyptivia targeted knock-in of in-frame reporter constructs to the native coding sequence of broadly expressed neural genes with CRISPR/Cas9. Two of five attempts were successful, resulting in aSyt1:GCaMP6sstrain that expresses synaptically-localized GCaMP in all neurons and abrp-T2A-QF2wdriver strain that can be used to drive and amplify expression of any effector in all neurons via the Q binary system. We show that both manipulations broadly and uniformly label the nervous system and have only mild effects on behavior. We envision that these strains will facilitate neurobiological research inAe. aegyptimosquitoes and provide documentation of both successful and failed manipulations as a roadmap for similar tool development in other non-model species.
Attribution of genetic engineering: A practical and accurate machine-learning toolkit for biosecurity
Authors: Alley Ethan C. , Turpin Miles , Liu Andrew Bo , Kulp-McDowall Taylor , Swett Jacob , Edison Rey , Von Stetina Stephen E. , Church George M. , Esvelt Kevin M. 
Published: August 2020

AbstractThe promise of biotechnology is tempered by its potential for accidental or deliberate misuse. Reliably identifying telltale signatures characteristic to different genetic designers, termedgenetic engineering attribution, would deter misuse, yet is still considered unsolved. Here, we show that recurrent neural networks trained on DNA motifs and basic phenotype can reach 70% attribution accuracy distinguishing between over 1,300 labs. To make these models usable in practice, we introduce a framework for weighing predictions against other investigative evidence using calibration, and bring our model to within 1.6% of perfect calibration. Additionally, we demonstrate that simple models can accurately predict both the nation-state-of-origin and ancestor labs, forming the foundation of an integrated attribution toolkit which should promote responsible innovation and international security alike.
Single-component multilayered self-assembling nanoparticles presenting rationally designed glycoprotein trimers as Ebola virus vaccines
Authors: He Linling , Chaudhary Anshul , Lin Xiaohe , Sou Cindy , Alkutkar Tanwee , Kumar Sonu , Ngo Timothy , Kosviner Ezra , Ozorowski Gabriel , Stanfield Robyn L. , Ward Andrew B. , Wilson Ian A. , Zhu Jiang 
Published: August 2020

AbstractEbola virus (EBOV) glycoprotein (GP) can be recognized by neutralizing antibodies (NAbs) and is the main target for vaccine design. Here, we first investigate the contribution of the stalk and heptad repeat 1-C (HR1C) regions to GP metastability. Specific stalk and HR1Cmodifications in a mucin-deleted form (GPΔmuc) increase trimer yield, whereas alterations of HR1Cexert a more complex effect on thermostability. Crystal structures are determined to validate two rationally designed GPΔmuc trimers in their unliganded state. We then display a modified GPΔmuc trimer on reengineered nanoparticles that encapsulate a layer of locking domains (LD) and a cluster of helper T-cell epitopes. In mice and rabbits, GP trimers and nanoparticles elicit cross-ebolavirus NAbs, as well as non-NAbs that enhance pseudovirus infection. Repertoire sequencing reveals quantitative profiles of vaccine-induced B-cell responses. This study demonstrates a promising vaccine strategy for filoviruses, such as EBOV, based on GP stabilization and nanoparticle display.
AnEngrailed1enhancer underlies human thermoregulatory evolution
Authors: Aldea Daniel , Atsuta Yuji , Kokalari Blerina , Schaffner Stephen , Warder Bailey , Kamberov Yana 
Published: August 2020

SummaryHumans rely on sweating to cool off and have the highest eccrine sweat gland density among mammals. We investigated whether altered regulation of theEngrailed 1(EN1) gene, the levels of which are critical for patterning eccrine glands during development, could underlie the evolution of this defining human trait. First, we identify fiveEN1candidate enhancers (ECEs) using comparative genomics and validation of enhancer activity in mouse skin. The human ortholog of one ECE, hECE18, contains multiple derived substitutions that together dramatically increase the activity of this enhancer in keratinocytes. Targeted repression of hECE18 reducesEN1expression in human keratinocytes, indicating hECE18 upregulatesEN1in this context. Finally, we find that hECE18 increases ectodermalEn1in a humanized knock-in mouse to increase eccrine gland number. Our study uncovers a genetic basis for the evolution of one of the most singular human adaptations and implicates the recurrent mutation of a single enhancer as a novel mechanism for evolutionary change.
Multiplex recombinase polymerase amplification (RPA) assay developed using unique genomic regions and coupled with a lateral flow device for rapid on-site detection of genusClavibacterandC. nebraskensis
Authors: Larrea-Sarmiento Adriana , Stack James P. , Alvarez Anne M. , Arif Mohammad 
Published: August 2020

ABSTRACTClavibacteris an agriculturally important bacterial genus comprising nine host-specific species/subspecies includingC. nebraskensis(Cn), which causes Goss’s wilt and blight of maize. A robust, simple, and field-deployable method is required to specifically detectCnin infected plants and distinguish it from otherClavibacterspecies for quarantine purposes and timely disease management. A multiplex Recombinase Polymerase Amplification (RPA) coupled with a Lateral Flow Device (LFD) was developed for sensitive and rapid detection ofClavibacterandCndirectly from infected host. Unique and conserved genomic regions, the ABC transporter ATP-binding protein CDS/ABC-transporter permease and the MFS transporter gene, were used to design primers/probes for specific detection of genusClavibacterandCn, respectively. The assay was evaluated using 52 strains, representing all nine species/subspecies ofClavibacter, other closely related bacterial species, and naturally- and artificially-infected plant samples; no false positives or negatives were detected. The RPA reactions were also incubated in a closed hand at body temperature; results were again specific. The assay does not require DNA isolation and can be directly performed using host sap. The detection limit of 10 pg and 100 fg was determined forClavibacter- andCn-specific primers/probes, respectively. The detection limit forCn-specific primer/probe set was decreased to 1,000 fg when 1 µL of host sap was added into the RPA reaction containing 10-fold serially diluted genomic DNA; though no effect was observed onClavibacter-specific primer/probe set. The assay is accurate and has applications at point-of-need diagnostics. This is the first multiplex RPA for any plant pathogen.IMPORTANCEClavibacterspecies are prevalent worldwide as have the potential to result in systemic infection. In the past, detection attempts have relied on both molecular- and immunological-based assays; however, current detection methods are time consuming and laborious. Field-deployable tests are desirable to identify potential samples infected withClavibacterspecies. This study demonstrates that the field-deployable isothermal multi-target recombinase polymerase amplification can be performed for the simultaneous detection of the genusClavibacterin general (all species), andC. nebraskensis, in particular, without specialized equipment. Additionally, the multiplex RPA coupled with a LFD may confer the benefits of faster detection and discrimination ofClavibacterspecies that affect critical regions susceptible to infection. This user-friendly format offers a flexible assay to complement both nucleic acid amplification and novel diagnosis methods without the need for DNA purification; this assay may serve as a point-of-reference for developing multiplex RPA assay for other plant pathogens.
Impulse buying in social commerce: bundle offer, top reviews, and emotional intelligence
Authors: Zafar Abaid Ullah , Qiu Jiangnan , Shahzad Mohsin , Shen Jie , Bhutto Tahseen Ahmed , Irfan Muhammad 
Published: August 2020

PurposeConsidering the rapid adoption of social media among consumers and organizations, this study intends to examine the impact of online bundle promotions and contextual interactions on impulse buying as consumers encounter them synchronously. Hence, a research model is proposed with the integration of perceived transaction value, perceived acquisition values, top reviews information, impulse buying tendency and emotional intelligence following the stimulus-organism-response framework, promotional framing effect, and theory of selective attention.Design/methodology/approachData were collected from the active social media members of organization pages and selling groups by utilizing the self-administered questionnaire. This study employed the partial least squares structural equation modeling to evaluate the data of 358 individuals.FindingsResults reveal the positive impact of targeted constructs on the urge to buy impulsively with complementary partial mediation of impulse buying tendency. Besides, emotional intelligence dissuades users' impulse buying tendencies, but unexpectedly, its moderating effect is insignificant. Further, importance-performance map analysis highlights the highest importance of impulse buying tendency and better performance of perceived transaction value for the urge to buy impulsively.Originality/valueThis research is one of the early studies to explore the influence of social media advertising and contextual social factors (e.g. bundle offers and top reviews information) on impulse buying with the moderation of emotional intelligence and mediation of impulse buying tendency. This research is imperative for scholars and managers with pertinent suggestions to arouse impulse buying.
Seven-band ultra-thin terahertz metamaterial absorber at 0.3-0.5 THz frequency
Authors: A. Elakkiya , Sankararajan Radha , B.S. Sreeja 
Published: August 2020

Purpose
The proposed structure consists of copper as a ground plane and 50 µm polyimide dielectric layer is placed in between the ground panel and top radiating patch. Octagon and pentagon shapes are combined to form a unit cell. This structure exhibits seven absorption peaks within the short frequency range 0.3–0.5 terahertz (THz) without any interference. Under normal incidence, this structure achieves the absorption of 96.9%, 95.3%, 98.7%, 91.7%, 96.5%, 95% and 97.8% at 0.3136 THz, 0.377 THz, 0.4060 THz, 0.4085 THz, 0.4240 THz, 0.4436 THz and 0.4648 THz, respectively. This study aims to provide a range of applications in THz dielectric sensing, thickness sensing, communications, wavelength selective radiation and detecting.

Design/methodology/approach
Multi-band THz metamaterial absorbers (MMA) from previous research are having a large unit size because of the presence of stacked layers and multiple resonators within a single unit cell. This leads to difficulty while implementing in practical applications. In this study, a new MMA has been presented at seven distinct frequencies without using stacked layers and multiple resonators.

Findings
This structure exhibits seven absorption peaks within the short frequency range 0.3–0.5 THz without any interference. Under normal incidence this structure achieves the absorption of 96.9%, 95.3%, 98.7%, 91.7%, 96.5%, 95% and 97.8% at 0.3136 THz, 0.377 THz, 0.4060 THz, 0.4085 THz, 0.4240 THz, 0.4436 THz and 0.4648 THz, respectively. The polarization and angle insensitivity of the design have been validated by numerical simulation up to 90° of oblique incidence. The effects of variation in geometrical parameters on absorption response are demonstrated. The physical mechanism of the structure is analysed by electric and magnetic field distributions. The resonant frequency ranges and the number of bands in this work are compared with previously reported papers. In THz range, this is the first time a single planar structure provides seven-band high-level absorption performance.

Originality/value
The highlights of the proposed seven-band THz MMA structure, in comparison with previous THz metamaterials, are as follows: this has a simple unit-cell structure and high resonant mechanism within the short frequency range 0.3–0.5 THz; this MMA can provide seven-band high-level absorption performance in a single planar structure for the first time in THz range; and this structure is polarization and incident angle independent in nature.
Patterns and trends in Internet of Things (IoT) research: future applications in the construction industry
Authors: Ghosh Arka , Edwards David John , Hosseini M. Reza 
Published: August 2020

PurposeThe Internet of Things (IoT) provides exciting opportunities for the construction industry to solve its time and resource constraints and frequent defaults. This study seeks to identify and rank the perceived importance level of principal research areas associated with the IoT and the construction industry by utilising a scientific mapping tool (i.e. VOSviewer). Such knowledge would enable key drivers for successful adoption of the IoT and digitisation technologies to be outlined. An analysis of key drivers and research trends that facilitates the development of a roadmap for applying the IoT and digital technologies in the construction sector is therefore much needed.Design/methodology/approachAn interpretivist philosophical lens was adopted to analyse published work as secondary data, where each publication represented a unit of analysis. A total of 417 peer-reviewed journal review articles covering the IoT within the construction domain were systematically reviewed using a mixed-methods approach, utilising qualitative-scientometric analyses techniques.FindingsThe results revealed a field of study in a fledgling stage, with a limited number of experts operating somewhat in isolation and offering single-point solutions instead of taking an integrated “holistic” approach. Key publication outlets were identified and the main focus of research undertaken being in the technical areas of smart buildings, smart construction objects and environmental sustainability. The major effects of adopting the IoT within the construction industry were identified as high-speed reporting, complete process control, data explosion leading to deep data analytics, strict ethical and legal expectations. Key drivers of the IoT adoption were outlined: interoperability; data privacy and security; flexible governance structures; proper business planning and models.Practical implicationsThe study benefits researchers and industry practitioners alike. For researchers, the identified gaps reveal areas of high priority in future research. For construction companies, particularly small to medium-sized businesses, the study raises awareness of the latest developments and potential applicability of the IoT in the industry. For government agencies and policymakers, this study offers a point of reference in directing the adoption of the IoT smoothly in the construction sector and provides guidelines and standards for maximising the potential benefits.Originality/valueThe study is the first scientometric review of the existing body of knowledge in the context of application of the IoT in the construction industry. Findings expose knowledge gaps in contemporary research, specifically, a broader consideration of organisational adjustments needed to accommodate the IoT usage, economic analyses and impediments to wider acceptance.
Standing at the crossroads: the vulnerabilities of Australian business schools
Authors: Hogan Owen , Kortt Michael A. , Charles Michael B. 
Published: August 2020

PurposeTo identify key factors that are contributing to vulnerability in business schools in Australian public universities and determine the degree of vulnerability exhibited by these schools.Design/methodology/approachUsing a multi-criteria assessment (MCA) approach, a framework is developed to determine which business schools are most vulnerable to disruptions and uncertainty.FindingsThe findings show a lack of preparedness on the part of many business schools, particularly those relying heavily on international students.Practical implicationsThe implication is that business schools in Australian public universities need to diversify revenue streams and continue to seek legitimacy through external stakeholders such as employers and international accrediting bodies.Originality/valueThis study presents an empirical perspective of business schools in Australian public universities and offers valuable insights for university leaders and policymakers.
Comparing user satisfaction of older and newer on-campus accommodation buildings in Australia
Authors: Xu Xinyu , Sunindijo Riza Yosia , Mussi Eveline 
Published: August 2020

Purpose
This paper aims to assess the level of occupants’ satisfaction, comparing older and newer on-campus accommodation buildings in Sydney, Australia, aiming to identify their comfort factors deficiencies in terms of design and construction solutions/strategies (e.g. spatial arrangements, materials, thermal comfort).

Design/methodology/approach
A post occupancy evaluation survey was used to assess the occupant satisfaction with three on-campus accommodation buildings in The University of New South Wales (UNSW) Sydney. One of the selected buildings is an older building opened for occupation in 1996, and the other two are more recent on campus accommodations buildings. The survey included 11 post occupancy evaluation elements identified through literature review which were categorised into three dimensions: technical, functional and behavioural.

Findings
The results show that the satisfaction levels with thermal and acoustic comfort were below standards for both older and newer buildings. In addition, the older building used in this study was rated low in terms of: indoor air quality, lighting, maintenance and management, vertical transportation facility, room layout and furniture quality, building layout and aesthetics and level of privacy. Such factors related to both functional and behavioural dimensions were of greater satisfaction in newer buildings.

Practical implications
Findings suggest the high priority of strategies that address and improve the thermal and acoustic comfort of older and newer on-campus accommodation buildings if the intention is to enhance students’ satisfaction, especially considering the impact that these facilities have on students’ performance. Thermal performance in different seasons and adaptive thermal comfort activities should be considered in the design of new on-campus accommodation buildings and the retrofit of existing old buildings.

Originality/value
On-campus accommodation is an important facility that supports student learning outcomes and helps students adapt in a new learning environment. A post occupancy evaluation study to assess the adequacy of this facility is still lacking because previous studies have generally focussed on class rooms and work spaces in the education sector. This research compares the user satisfaction of older and newer on-campus accommodation buildings in Australia, to highlight deficiencies and areas for improvement in the design of existing and future buildings.
Transnational ties and performance of immigrant firms: evidence from Central Italy
Authors: Brzozowski Jan , Cucculelli Marco 
Published: August 2020

PurposeThe purpose of this study is to analyze the determinants of transnational business ties (i.e. ties with the country of origin) and to investigate their impact on the economic performance of immigrant firms.Design/methodology/approachBased on the representative survey conducted among 259 foreign-born immigrant entrepreneurs in Marche region (Central Italy), the study investigates the determinants of having transnational ties with countries of origin. Then it compares the effect of transnational ties and the effect of alternative domestic ethnic business ties on the performance of immigrant firms.FindingsThis study demonstrates that having transnational ties is more likely for individuals with former entrepreneurial experience in the home countries and with higher education acquired in Italy. The performance of immigrant firms in the 2014–2016 period (measured by the level of revenues) is positively related to transnational ties – this effect is very strong and significant. On the other hand, reliance on the domestic ethnic ties (i.e. ties with co-ethnic population in Italy) is one of the key factors that inhibits further growth of immigrant enterprises.Originality/valueThis study advances the understanding of how immigrant entrepreneurs create transnational business ties with their home countries. Additionally, this research contributes to the existing literature on immigrant entrepreneurship, by explaining the role of heterogeneous business ties: transnational connections, domestic ties with co-ethnic partners and ties with individuals from native population (in this case: Italy) for the entrepreneurial performance. Consequently, it offers recommendations for policy-makers willing to encourage entrepreneurial activities of immigrants in host countries and to their counterparts in home countries, who are planning to use the advantage diaspora entrepreneurial potential for the benefit of their economies.