bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceptions and predictors of organizational justice among healthcare professionals in academic hospitals in South-eastern Nigeria
Authors: Ghasi Nwanneka Chidinma , Ogbuabor Daniel Chukwuemeka , Onodugo Vincent Aghaegbunam 
Published: April 2020

Abstract
Background Research on organizational justice in hospitals in African countries are limited despite being important for workforce performance and hospital operational efficiency. This paper investigated perceptions and predictors of organizational justice among health professionals in academic hospitals in South-east Nigeria. Methods The study was conducted in two teaching hospitals in Enugu State, South-east Nigeria using mixed-methods design. Randomly sampled 360 health professionals (doctors = 105, nurses = 200 and allied health professionals, AHPs = 55) completed an organizational justice scale. Additionally, semi-structured, in-depth interview with purposively selected 18 health professionals were conducted. Univariate and bivariate statistics and multivariable linear regression were used to analyze quantitative data. Statistical significance was set at alpha 0.05 level. Qualitative data were analyzed thematically using NVivo 11 software. Results The findings revealed moderate to high perception of different dimensions of organizational justice. Doctors showed the highest perception, whereas AHPs had the least perception. Among doctors, age and education predicted distributive justice (adjusted R2 = 22%); hospital ownership and education predicted procedural justice (adjusted R2 = 17%); and hospital ownership predicted interactional justice (adjusted R2 = 42%). Among nurses, age, gender and marital status predicted distributive justice (adjusted R2 = 41%); hospital ownership, age and gender predicted procedural justice (adjusted R2 = 28%); and hospital ownership, age, marital status and tenure predicted interactional justice (R2 = 35%). Among AHPs, marital status predicted distributive justice (adjusted R2 = 5%), while hospital ownership and tenure predicted interactional justice (adjusted R2 =15%). Qualitative findings indicate that nurses and AHPs perceive as unfair, differences in pay, access to hospital resources, training, work schedule, participation in decision-making and enforcement of policies between doctors and other health professionals due to medical dominance. Overall, supervisors have a culture of limited information sharing with, and disrespectful treatment of, their junior colleagues. Conclusion Perceptions of organizational justice range from moderate to high and predictors vary among different healthcare professionals. Addressing specific socio-demographic factors that significantly influenced perceptions of organizational justice among different categories of health professionals and departure from physician-centered culture would improve perceptions of organizational justice among health professionals in Nigeria and similar settings.
Patient-specific instrument assisted calcaneal fracture minimally invasive internal fixation can carry out preoperative plan accurately and quickly: a retrospective study
Authors: Wang Chenggong , Xu Can , Li Mingqing , Li Hui , Xiao Han , Zhong Da , Liu Hua 
Published: April 2020

Abstract
Background: Almost all of the traditional calcaneal fracture minimally invasive internal fixations (MIIF) require a lot of intraoperative fluoroscopy, moreover, the fracture recovery of many patients is not considered ideal. Therefore, we designed a new surgical procedure through the digital surgical simulation and made patient-specific instrument (PSI) for calcaneal fracture patients before surgery, and then we manage the operation through the assistance of PSI during the whole process. The purpose of this study is to verify whether the PSI assisted calcaneal fracture minimally invasive internal fixation can carry out preoperative plan accurately and quickly. Methods: We retrospectively analyzed the Sanders type III or IV fresh calcaneal fracture patients who had been performed MIIF assisted by the PSI in our hospital during January 2016 to December 2018. We collected and analyzed perioperative data including intraoperative fluoroscopy times, whether the internal fixation actual usage (IFAU) same as preoperative plan, surgical time and situation of complication; and collected and analyzed the data of preoperative actual measurement, preoperative-plan and postoperative actual measurement from X-rays and CT, including Böhler angle, Gissane angle, subtalar joint width, calcaneus valgus angle and calcaneal volume overlapping ratio. All patients had been followed up and their AOFAS score had been recorded. Then we performed statistical analyses. Results: The intraoperative fluoroscopy times was 3.95±1.78, the condition of IFAU in 16 patients (16 feet) was the same as preoperative plan, the surgery time was 28.16±10.70 min, no patients developed complications. The preoperative plan Böhler angle, Gissane angle, subtalar joint width and calcaneus valgus angle were not significant different from the postoperative; the preoperative Böhler angle, Gissane angle, subtalar joint width and calcaneus valgus angle were all significant different from the postoperative. The calcaneal volume overlapping ratio with preoperative design was 91.2±2.3%. The AOFAS score of all patients gradually increased as time goes on, and difference of the AOFAS scores between all time-points were significant. Conclusions: The new patient-specific instrument assisted calcaneal fracture minimally invasive internal fixation can carry out the preoperative plan accurately and quickly.
High Hip Center Technique in Total Hip Arthroplasty for Crowe Type II-III Developmental Dysplasia: Results of Midterm Follow-up
Authors: Shen Junmin , Zhou Yonggang , Sun Jingyang , Ma Haiyang , Du Yinqiao , Li Tiejian 
Published: April 2020

Abstract
Background: High hip center technique is still controversial about the survivorship of prothesis and postoperative complications. We aimed to show the utility of high hip center technique used in patients with Crowe II-III developmental dysplasia of the hip at the midterm follow-up and evaluated the clinical and radiographic results between different heights of hip center.Methods: We retrospectively evaluated 69 patients (85 hips) with Crowe II-III dysplasia who underwent a high hip center cementless total hip arthroplasty at a mean follow up of 8.9 years (range, 6.0-14.1years). The patients were divided into two groups according to the height of hip center, respectively group A (≥22mm and <28mm) and group B (≥28mm). Radiographic, functional and survivorship outcomes were evaluated.Results: There were no statistically significant differences between two groups in horizontal distance, offset, abductor lever arm, leg length discrepancy and cup inclination. At the final follow up, the mean WOMAC and Harris hip score were significantly improved in both groups. Of the 85 hips, 7 hips (8.2%) showed a positive Trendelenburg sign. Besides, 6 patients (8.7%) presented with a limp. No significant differences were shown regarding the Harris hip score, WOMAC score, Trendelenburg sign and limp between two groups. The Kaplan-Meier implants survivorship rates at the final follow-up for all-causes revisions in group A and group B were similar (96.7% [95% confidence interval, 90.5%-100%] and 96.2% [95% confidence interval, 89.0%-100%], respectively).Conclusions: The high hip center technique is a valuable alternative to achieve excellent midterm results for Crowe II-III developmental dysplasia of the hip.
Full-length transcriptome sequencing of the identified fruit shape-related genes of the Olecranon honey peach
Authors: Liu Jianliang , Bao Yao , Wang Qin , Liu Huifan 
Published: April 2020

Abstract
Background: The Olecranon honey peach shaped like an eagle’s beak. In this study, full-length transcriptome sequencing of the Olecranon honey peach and its bud mutant variant was performed by Oxford Nanopore Technologies to comparatively analyze the differentially expressed genes and the transcriptome to identify important fruit shape-related genes. Results: Full-length transcriptome sequencing was performed to analyze the two peaches. A consensus isoform was obtained and compared to the reference genome for an elimination of redundancy analysis. As a result, a final set of 58 596 transcript sequences was obtained. A total of 21 745 simple sequence repeats were obtained, and 18 322 alternative splicing (AS) events were identified. The comparative analysis of the non-redundant transcript revealed 2530 new gene loci and 37 364 novel transcripts. A total of 457 genes were differentially expressed in the two groups, including 169 up-regulated genes and 288 down-regulated genes. A total of 1519 transcripts were differentially expressed, of which 552 were up-regulated and 997 were down-regulated. Conclusions: In the case of the plant hormone signal transduction pathway identified by the KEGG annotation, our analyses revealed that differential expression of genes 9229 , 26004 , 22504 , 2822 , 2826 , 2824 , ONT.1953 , ONT.1950 , and ONT.1953 was related to the shape of the peach and may regulate the production of large fruit, via endogenous hormones, secondary metabolites, and signal transduction. This study provides useful information on the shape-related genes and transcripts in the Olecranon honey peach.
Construction of SSR linkage maps and identification of QTL for resistance to root rot in sweetpotato (Ipomoea batatas (L.) Lam.)
Authors: Ma Zhimin , Gao Wenchuan , Liu Lanfu , Liu Minghui , Zhao Ning , Han Meikun , Wang Zhao , Jiao Weijing , Gao Zhiyuan , Hu Yaya , Liu Qingchang 
Published: April 2020

Abstract
Background: Sweetpotato root rot is a devastating disease caused by Fusarium solani that causes significant yield losses of sweetpotato in China. There is currently no effective method to control the disease. The breeding of resistant varieties is the most effective and economic way to control the disease. To date, quantitative trait locus (QTL) for resistance to root rot have not been reported and the biological mechanisms of resistance remain unclear in sweetpotato. Thus, it is necessary and worthwhile to identify resistance loci to help develop disease-resistant varieties.
Results: In this study, we constructed genetic linkage maps of sweetpotato using a mapping population consisting of 300 individuals derived from a cross between Jizishu 1 and Longshu 9 by simple sequence repeat (SSR) markers, and mapped seven QTLs for resistance to root rot. In total, 484 and 573 polymorphic SSR markers were grouped into 90 linkage groups for Jizishu 1 and Longshu 9, respectively. The total map distance for Jizishu 1 was 3,974.24 cM, with an average marker distance of 8.23 cM. The total map distance for Longshu 9 was 5,163.35 cM, with an average marker distance of 9.01 cM. Five QTLs ( qRRM_1 , qRRM_2 , qRRM_3, qRRM_4 , and qRRM_5 ) were located in five linkage groups of Jizishu 1 map explaining 52.6-57.0% of the variation. Two QTLs ( qRRF_1 and qRRF_2 ) were mapped on two linkage groups of Longshu 9 explaining 57.6% and 53.6% of the variation. 71.4 % of the QTLs had a positive effect on the variation. Three of the seven QTLs, qRRM_3 , qRRF_1 , and qRRF_2 , were colocalized with markers IES43-5mt, IES68-6fs**, and IES108-1fs, respectively.
Conclusions: To our knowledge, this is the first report on the construction of a genetic linkage map for purple sweetpotato (Jizishu 1) and the identification of QTLs associated with resistance to root rot in sweetpotato using SSR markers. These QTLs will have practical significance for the fine mapping of root rot resistance genes and play an important role in sweetpotato marker-assisted breeding.
Development of a precision medicine pipeline to identify personalized treatments for colorectal cancer
Authors: Altunel Erdem , Roghani Roham S. , Chen Kai-Yuan , Kim So Young , McCall Shannon , Ware Kathryn E. , Shen Xiling , Somarelli Jason A. , Hsu David S. 
Published: April 2020

Abstract
Background: Metastatic colorectal cancer (CRC) continues to be a major health problem, and current treatments are primarily for disease control and palliation of symptoms. In this study, we developed a precision medicine strategy to discover novel therapeutics for patients with CRC. Methods: Six matched low-passage cell lines and patient-derived xenografts (PDX) were established from CRC patients undergoing resection of their cancer. High-throughput drug screens using a 119 FDA-approved oncology drug library were performed on these cell lines, which were then validated in vivo in matched PDXs. RNA-Seq analysis was then performed to identify predictors of response. Results: Our study revealed marked differences in response to standard-of-care agents across patients and pinpointed druggable pathways to treat CRC. Among these pathways co-targeting of fibroblast growth factor receptor (FGFR), SRC, platelet derived growth factor receptor (PDGFR), or vascular endothelial growth factor receptor (VEGFR) signaling was found to be an effective strategy. Molecular analyses revealed potential predictors of response to these druggable pathways. Conclusions: Our data suggests that the use of matched low-passage cell lines and PDXs is a promising strategy to identify new therapies and pathways to treat metastatic CRC.
The MD-PhD Program In Geneva: A 10-year Analysis Of Demographics And Outcomes
Authors: Rocha Andre Dos Santos , Combescure Cristophe , Negro Francesco 
Published: April 2020

Abstract
Background: MD-PhD programs are research-oriented degrees that empower medical doctors with in-depth scientific skills. ‘Physician-scientists’ graduating from these programs are expected to highly contribute to biomedical research and academic medicine, alongside clinical practice. In that respect, however, there is limited knowledge about the outcomes and careers of the MD-PhD workforce, particularly in Europe. In the present study, we surveyed the MD-PhD graduates from the University of Geneva between 2010 and 2019.Methods: A cross-sectional survey was performed in April 2019, targeting all medical doctors who had obtained the MD-PhD degree from the University of Geneva since 2010. Features, career outcomes and opinions of MD-PhD graduates were assessed through an online anonymous questionnaire.Results: Twenty-one questionnaires were collected from 31 MD-PhD graduates (response rate 65.5 %). Most respondents (57.1%) had performed an MD-PhD training in basic sciences; however, only 14.3% had pursued this type of research thereafter. Most of the respondents were holding a position at a University hospital (90.5%), although a significant number of them were no longer doing research in their current position (28.6%) and 85.7% were facing obstacles and challenges to combine clinical duties and research. Despite this, the majority declared that the MD-PhD degree had given them an edge during the career (85.7%). Conclusions: The physician-scientists who graduated from the MD-PhD program in Geneva were globally satisfied with their training. However, many challenges and obstacles to combine clinical duties and research were reported by MD-PhD graduates, precluding the implementation of research activities in their current position.
Network Pharmacology and Molecular docking Analysis on Mechanisms and Molecular Targets of LongChaiJiangXue Formula for Treatment of Polycythemia Vera
Authors: Ming Jing , Zhu Shirong , Li Yujin , Liu Weiyi , Jiang Yunyao , Hu Xiaomei 
Published: April 2020

Abstract
Background: LongChaiJiangXue formula (LCJX) has the effect of not only clearing up excessive erythrocytes but also relieving clinical symptoms of Polycythemia vera (PV). Material/Methods: The chemical constitution of LCJX was identified from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). Seven hundred and fifty-nine targets were identified and a total of 248 targets were screened out after discarding duplication and genes without any ID in databases. GeneCards database, OMIM database, and GEO database were searched for differential expression genes. The network was built by Cytoscape (3.7.2) software and the protein-protein interaction (PPI) networks of PV and LCJX were merged by https://string-db.org . Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment was processed by the R platform. The molecular docking technology was used to further analyze the intense of the assosiation of the compounds and targets. Results: 73 compounds of LCJX were chosen as the candidate active compounds. The compound-targets network contained 105 nods and 216 edges that presented the interaction of agents and targets. The PPI network of LCJX targets involved 59 nodes and 168 edges. The network showed that the key nodes were concentrated in signal transducer and activator of transcription-3(STAT-3), interleukin-6(IL-6), Janus kinase 2(JAK2), and vascular endothelial growth factor-A(VEGFA). The most enriched terms in the GO analysis in the GO biological processes(BP) were reactive oxygen species metabolic process, response to lipopolysaccharide, and response to oxidative stress. According to GO molecular functions(MF), the central nodes were generally enriched in cytokine receptor binding, cytokine activity, and heme binding. Regarding the GO cell components(CC), the terms included vesicle lumen, cytoplasmic vesicle lumen, and secretory granule lumen. In light of the KEGG enrichment analysis, the Hepatitis B, AGE-RAGE signaling pathway in diabetic complications, Kaposi sarcoma-associated herpesvirus infection, measles, Human cytomegalovirus infection, and JAK-STAT signaling pathway were significantly enriched. The molecular docking technology found that puerarin and saikosaponin A had relative stronger affinity with VEGFA, HIF-1A, JAK2 and STAT3 than other compounds in terms of the binding free energy. Conclusions: The effect of LCJX on PV is achieved through a series of complex mechanisms. Network pharmacology and molecular docking are powerful tools to reveal the effect of compound Chinese medicine on the disease.
A Confirmatory Factor Analysis of the knowledge, attitude and practice questionnaire towards prevention of Respiratory tract infections during Hajj and Umrah
Authors: Goni Mohammd Dauda , Naing Nyi Nyi , Hasan Habsah , Wan-Arfah Nadiah , Deris Zakuan Zainy , Arifin Wan Nor , Baaba Aisha Abubakar , Njaka Stanley 
Published: April 2020

Abstract
Background Respiratory tract infections are one of the common infection associated with Hajj pilgrimage that is of great public health and global concern. This study is aimed at determining the factor structure of the knowledge, attitude and practice questionnaire for the prevention of respiratory tract infections during Hajj by confirmatory factor analysis (CFA). Methods A multistage cluster sampling method was conducted on Malaysian Umrah pilgrims during the weekly Umrah orientation course. A total of 200 Umrah pilgrims participated in the study. The KAP questionnaire was distributed to pilgrims at the beginning of the orientation and retrieved immediately at the end of the orientation. The robust maximum likelihood was used for the estimation due to the multivariate normality assumption violation. A two-factor model was tested for measurement model validity and construct validity for each of the attitude and practice domains. Results CFA of a 25-item in total, two-factor model yielded adequate goodness-of-fit values. The measurement model also showed a good convergent and discriminant validity after model re-specification. A two-factor model was tested for measurement model validity and construct validity for each of the attitude and practice domains. Conclusion The KAP questionnaire was proven to have a valid measurement model and reliable constructs. It was deemed suitable for use to measure the KAP of Hajj and Umrah pilgrims towards the prevention of respiratory tract infections.
Exploration of a novel prognostic risk signatures and immune checkpoint molecules in endometrial carcinoma microenvironment
Authors: Liu Jinhui , Nie Sipei , Wu Zhipeng , Li Siyue , Jiang Yi , Wan Yicong , Meng Huangyang , Zhou Shulin , Cheng Wenjun 
Published: April 2020

Abstract
Background: Endometrial carcinoma (EC) is one of the common female malignancies. The effectiveness of immunotherapy in treating EC remains to be testified. Methods: In our present study, we explored the underlying mechanism through tumor microenvironment (TME) and immune-related genes in EC. Based on The Cancer Genome Atlas (TCGA) database to screen immune-related differentially expressed genes (DEGs). Then, survival analysis, functional annotations and interpreted interaction network of these genes were further investigated for the underlying regulation mechanisms. Moreover, we used a conjoint Cox regression analysis to built the immune-related prognostic risk signatures and identify the candidate prognostic biomarkers. Based on the risk model, we further explored the predictive value of risk signatures by exploring the tumor-infiltrating immune cells, prognostic immune checkpoints modulators, and checkpoint inhibitors in TME. Results: As a result, we found 799 up-regulated and 139 down-regulated immune-related and differentially expressed genes of EC. Based on the 225 prognostic DEGs, we built two risk models based on key prognostic immune-related genes, which identified respectively for overall survival and disease-free survival. Gene set enrichment analysis revealed immune-related pathways were mostly enriched in gene sets of the low-risk group, and the nomograms were constructed based on the risk models. By analyzing the immune status in low- and high- group of EC patients , we found higher immune cell infiltration and activation in low risk tumor tissues, which might cause lower tumor purity and contribute to better prognosis of EC. By analyzing the immune checkpoint molecules, we found both the expression and immunophenoscore of immune checkpoints PD-1, CTLA4, PD-L1 and PD-L2 increased significantly in low-risk group. In addition, the low-risk group were found display higher tumor mutational burden. Conclusions: Our study suggested that high-immune TME might prevent the progression of EC, which indicated the possibility of immunotherapy to fight against EC.