bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of functional epithelial organoids from human lacrimal glands
Authors: Jeong Sang Yun , Choi Woo Hee , Jeon Seong Gyeong , Lee Sookon , Park Jong-Moon , Park Mira , Lee Hookeun , Lew Helen , Yoo Jongman 
Published: January 2021

Abstract
Background: Tear deficiency due to dysfunction of the lacrimal gland (LG) is one of the major causes of dry eye disease (DED). Therefore, LG stem cell-based therapies have been extensively reported to regenerate injured lacrimal tissue, but the number of stem cells in the LG tissue is pretty low, and 2D long-term cultivation results in the reduction of the differentiation capacity of stem cells. Whereas, 3D LG organoids could be an alternative for a DED therapeutic method, because it is capable of prolonged growth while maintaining the characteristics of the LG tissue. In this study, we developed LG organoids and applied them as cell therapeutics. Methods: Digested cells from human LG tissue were mixed with Matrigel and cultured in five different media modified from human prostate/salivary organoid culture media. After organoid formation, the growth, expression of specific markers and histological characters were analyzed to prove the LG organoids. And then, the secretory function of LG organoids was confirmed by calcium influx or proteomics after pilocarpine treatment. To explore the curability of developed organoids, finally, mouse-derived lacrimal gland organoids were fabricated and transplanted into mouse lacrimal tissue with DED. Results: The histological features and specific marker expression of LG organoids were similar to normal LG tissue. In the pilocarpine treated LG organoid, internal Ca2+ ions and β -hexosaminidase as known a lysosomal protein in tear fluid was increased. Also, the secreted proteins from pilocarpine treated lacrimal organoid were identified through proteomics. More than 70% of the identified proteins were proven to exosome through GO analysis. These results indicate that our developed organoid has a reactivity to pilocarpine, which shows the function of the lacrimal gland. Additionally, we developed LG organoids from Sjogren’s syndrome patients (SS) and confirmed that their histological feutures similar to that of SS-derived LG tissue. Finally, we confirmed that organoids were well engrafted in the lacrimal tissue at 2 weeks after transplantation of mouse LG orgnoid. Conclusion: This current study demonstrate that our established lacrimal gland organoids resemble characteristics of normal lacrimal gland tissue and could be used as cell therapy for patients with dry eye syndrome.
Linkage analysis, GWAS, transcriptome analysis to identify candidate genes for rice seedlings in response to high temperature stress
Authors: Wei Zhaoran , Yuan Qiaoling , Lin Hai , Li Xiaoxia , Zhang Chao , Gao Hongsheng , Zhang Bin , He Huiying , Liu Tianjiao , Zhang Jie , Gao Xu , Shi Shandang , Wang Bo , Gao Zhenyu , Kong Lingrang , Qian Qian , Shang Lianguang 
Published: January 2021

Abstract
Background: Rice plants suffer from the rising temperature which is becoming more and more prominent. Mining heat-resistant genes and applying them to rice breeding is a feasible and effective way to solve the problem.Result: Three main biomass traits, including shoot length, dry weight, and fresh weight, changed after abnormally high temperature treatment in the rice seedling stage of a recombinant inbred lines and germplasm population. Based on a comparison of the results of linkage analysis and genome-wide association analysis, two loci with lengths of 57 kb and 69 kb in qDW7 and qFW6, respectively, was associated with the rice response to abnormally high temperatures in the seedling stage. Meanwhile, based on integrated transcriptome analysis, some genes are considered as important candidate genes. Combining with known genes and analysis of homologous genes, it was found that there are eight genes in candidate intervals that need to be focused in subsequent research.Conclusions: The results indicated several relevant loci, which would help researchers to further discover beneficial heat-resistant genes that can be applied to rice heat-resistant breeding.
A semi-dominant mutation in OsCESA9 improves salt tolerance and favors field straw decay traits by altering cell wall properties in rice
Authors: Ye Yafeng , Wang Shuoxun , Wu Kun , Ren Yan , Jiang Hongrui , Chen Jianfeng , Tao Liangzhi , Fu Xiangdong , Liu Binmei , Wu Yuejin 
Published: January 2021

Abstract
Background: Cellulose synthase (CESA) mutants have potential use in straw processing due to their lower cellulose content, but almost all of the mutants exhibit defective phenotypes in plant growth and development. Balancing normal plant growth with reduced cellulose content remains a challenge, as cellulose content and normal plant growth are typically negatively correlated with one another. Result: Here, the rice (Oryza sativa) semi-dominant brittle culm (sdbc) mutant Sdbc1, which harbors a substitution (D387N) at the first conserved aspartic acid residue of OsCESA9, exhibits lower cellulose content and reduced secondary wall thickness as well as enhanced biomass enzymatic saccharification compared with the wild type (WT). Further experiments indicated that the OsCESA9D387N mutation may compete with the wild-type OsCESA9 for interacting with OsCESA4 and OsCESA7, further forming non-functional or partially functional CSCs. The OsCESA9/OsCESA9D387N heterozygous plants increase salt tolerance through scavenging and detoxification of ROS and indirectly affecting related gene expression. They also improve rice straw return to the field due to their brittle culms and lower cellulose content without any negative effects in grain yield and lodging. Conclusion: Hence, OsCESA9D387N allele can improve rice salt tolerance and provide the prospect of the rice straw for biofuels and bioproducts due to its improved enzymatic saccharification.
Perspectives of family physicians towards access to lung cancer screening for individuals living with low income - a qualitative study
Authors: Sayani Ambreen , Vahabi Mandana , O’Brien Mary Ann , Liu Geoffrey , Hwang Stephen W. , Selby Peter , Nicholson Erika , Lofters Aisha 
Published: January 2021

Abstract
Background: Individuals living with low income are less likely to participate in lung cancer screening (LCS) with low-dose computed tomography. Family physicians (FPs) are typically responsible for referring eligible patients to LCS; therefore, we sought to understand their perspectives on access to lung cancer screening for individuals living with low income in order to improve equity in access to LCS. Methods: A theory-informed thematic analysis was conducted using data collected from 11 semi-structured interviews with FPs recruited from three primary care sites in downtown Toronto. Data was coded using the Systems Model of Clinical Preventative Care as a framework and interpretation was guided by the synergies of oppression analytical lens. Results: Four overarching themes describe FP perspectives on access to LCS for individuals living with low income: the degree of social disadvantage that influences lung cancer risk and opportunities to access care; the clinical encounter, where there is often a mismatch between the complex health needs of low income individuals and structure of health care appointments; the need for equity-oriented health care, illustrated by the neglect of structural origins of health risk and the benefits of a trauma-informed approach; and finally, the multiprong strategies that will be needed in order to improve equity in health outcomes. Conclusion: An equity-oriented and interdisciplinary team based approach to care will be needed in order to improve access to LCS, and attention must be given to the upstream determinants of lung cancer in order to reduce lung cancer risk.
GESTÃO DE MEDICAMENTOS: ESTUDO DE CASO EM UMA UNIDADE DE SAÚDE DE MACEIÓ/AL
Authors: Levino Natallya , Brito Bruna Luana 
Published: January 2021

A economia em saúde vem, ao longo dos anos, ganhando relevância em virtude dos altos gastos da saúde pública e privada. Paralelamente a isso, vive-se o contraponto entre o aumento dos custos e gastos relacionados à assistência médico-hospitalar. Mas, como ofertar um serviço público assistencial de qualidade, com recursos escassos, reduzindo/mantendo custos, maximizando/mantendo resultados, sem, contudo, desconsiderar a busca pela eficiência e o anseio à modernização de suas estruturas física, humana e administrativa? O objetivo deste trabalho é analisar o processo de gestão de custos e estoques de medicamentos de uma unidade de saúde de Maceió/AL, tendo como unidade de estudo a Farmácia Hospitalar. Foi realizada uma pesquisa aplicada, descritiva, com abordagem qualitativa, cujo procedimento técnico foi o estudo de caso, durante os meses de Julho a Novembro de 2018. Constatou-se que as principais dificuldades referem-se à falta de integração entre os departamentos, as limitações de software de gerenciamento de estoques e custos, e principalmente, dificuldades de cunho financeiro, que impedem o relacionamento colaborativo com fornecedores e é calcado por restrições orçamentárias que prejudicam a prestação de serviços.
Research Domain Criteria (RDoC): A Perspective to Probe the Biological Background behind Treatment Efficacy in Depression
Authors: Calabrò Marco , Fabbri Chiara , Kasper Siegfried , Zohar Joseph , Souery Daniel , Montgomery Stuart , Albani Diego , Forloni Gianluigi , Ferentinos Panagiotis , Rujescu Dan , Mendlewicz Julien , Colombo Roberto , De Ronchi Diana , Serretti Alessandro , Crisafulli Concetta 
Published: January 2021

Background:
Major Depressive Disorder(MDD) and its frequent partial response to antidepressants
are a major health concern and therefore an important focus of research. Despite the efforts,
MDD pathogenesis and the mechanisms of antidepressant action are only partially understood. In the last
few years, the need of rethinking the classification of depressive disorders and psychiatric disorders, in
general, has been suggested, in order to provide a nosology that reflects more closely the biological background
associated with disease pathogenesis and its role/significance in treatment. The classification proposed
by the National Institute of Mental Health (NIMH), namely the research domain criteria (RDoC),
may represent a key framework to guide research in this direction.

Methods:
A literature search was performed on PubMed and Google Scholar databases in order to retrieve
data regarding Antidepressants effects on specific RDoC constructs. Further, the targets of drugs of
interest were identified through the Drug bank database, and their possible function within RDoC constructs
was discussed.

Discussion:
In this review, we summarize and discuss the significance of the results of pre-clinical and
clinical studies investigating specific RDoC paradigms relevant to depressive phenotypes and antidepressant
effects.

Conclusion:
The RDoC framework may facilitate a more specific use of antidepressants based on the individual’s
spectrum of symptoms and the development of new compounds that target specific depressive
symptoms.
The Therapeutic use of the Zonulin Inhibitor AT-1001 (Larazotide) for a Variety of Acute and Chronic Inflammatory Diseases
Authors: Troisi Jacopo , Venutolo Giorgia , Terracciano Concetta , Carri Matteo Delli , Di Micco Simone , Landolfi Annamaria , Fasano Alessio 
Published: January 2021

Background:
The involvement of intercellular tight junctions and, in particular,
the modulation of their competency by the zonulin pathway with a subsequent increase
in epithelial and endothelial permeability, has been described in several chronic
and acute inflammatory diseases. In this scenario, Larazotide, a zonulin antagonist, could
be employed as a viable therapeutic strategy.

Objective:
The present review aims to describe recent research and current observations
about zonulin involvement in several diseases and the use of its inhibitor Larazotide for
their treatment.

Methods:
A systematic search was conducted on PubMed and Google Scholar, resulting
in 209 publications obtained with the following search query: “Larazotide,” “Larazotide
acetate,” “AT-1001,” “FZI/0” and “INN-202.” After careful examination, some publications
were removed from consideration because they were either not in English or were
not directly related to Larazotide.

Results:
The obtained publications were subdivided according to Larazotide’s mechanism
of action and different diseases: celiac disease, type 1 diabetes, other autoimmune
diseases, inflammatory bowel disease, Kawasaki disease, respiratory (infective and/or
non-infective) diseases, and other.

Conclusion:
A substantial role of zonulin in many chronic and acute inflammatory diseases
has been demonstrated in both in vivo and in vitro, indicating the possible efficacy
of a Larazotide treatment. Moreover, new possible molecular targets for this molecule
have also been demonstrated.
Na, K-ATPase as a Biological Target for Gold(III) Complexes: A Theoretical and Experimental Approach
Authors: Bondžić Aleksandra M. , Vasić Anićijević Dragana D. , Janjić Goran V. , Zeković Ivana , Momić Tatjana , Nikezić Ana Vujačić , Vasić Vesna M. 
Published: January 2021

Background:
Gold-based complexes represent a new class of potential
metallodrugs. Although their action mechanism is not entirely understood, it was shown
that gold complexes inhibit some enzymes’ activities. Among them, Na,K-ATPase is
emerging as an essential target for various anticancer drugs. The functionalization of nanoparticles
by gold(III) complexes could facilitate their delivery into the cells and enable
the following of their distribution in the target tissues.

Objective:
The paper presents an overview of Na,K-ATPase interaction with representative
and structurally related cytotoxic gold(III) complexes. The results obtained by the
employment of theoretical methods (DFT and docking studies) combined with the experimental
approach involving a variety of nanotechnology-base techniques (UV/Vis, Raman
and fluorescence spectroscopy, CD, AFM, DLS) are discussed. Detailed information was
obtained on the enzyme’s conformational and structural changes upon binding the
gold(III) complexes. The experimentally determined reaction parameters (constants of
dissociation and the reaction stoichiometry) were predicted theoretically.

Conclusion:
The presented results offer further support to the view that Na,K-ATPase
may be a relevant biomolecular target for cytotoxic gold(III) compounds of medicinal interest.
Serum Uric Acid and Diabetes: From Pathophysiology to Cardiovascular Disease
Authors: Katsiki Niki , Dimitriadis George D. , Mikhailidis Dimitri P. 
Published: January 2021

:
Hyperuricemia, has been traditionally related to nephrolithiasis and gout. However, it has also been
associated with the development of type 2 diabetes mellitus (T2DM) and cardiometabolic and cardiovascular
diseases. Pathophysiologically, elevated serum uric acid (SUA) levels may be associated with abnormal lipid and
glucose metabolism.

:
In this narrative review, we consider the associations between hyperuricemia, hyperglycemia, atherosclerosis and
thrombosis. Furthermore, we comment on the available evidence linking elevated SUA levels with the incidence
and outcomes of coronary heart disease, stroke, peripheral artery disease and non-alcoholic fatty liver in subjects
with T2DM. The effects of antidiabetic drugs (e.g. metformin, pioglitazone, sulfonylureas, dipeptidyl peptidase 4
inhibitors, glucagon-like peptide-1 receptor agonists, sodium-glucose cotransporter 2 inhibitors and insulin) on
SUA concentrations are also reviewed.
Modulation of Drug Resistance by Limonene: Inhibition of Efflux Pumps in Staphylococcus aureus Strains RN-4220 and IS-58
Authors: de Araújo Ana C.J. , Freitas Priscilla R. , dos Santos Barbosa Cristina R. , Muniz Débora F. , Ribeiro-Filho Jaime , Tintino Saulo R. , Júnior José P.S. , Filho José M.B. , de Sousa Gabriela R. , Coutinho Henrique D.M. 
Published: January 2021

Aims::This study aimed to investigate the potential of limonene as an efflux pump (EP) inhibitor in Staphylococcus aureus strains, RN-4220 and IS-58, which carry EPs for erythromycin (MrsA) and tetracycline (TetK), respectively.Background::The evolution of bacterial resistance mechanisms over time has impaired the action of most classes of antibiotics. Staphylococcus aureus is a notable bacterium, with high pathogenic potential and demonstrated resistance to conventional antibiotics. Considering the importance of discovering novel compounds to combat antibiotic resistance, our group previously demonstrated the antibacterial properties of limonene, a compound present in the essential oils of several plant species.Objective::This study aimed to investigate the potential of limonene as an efflux pump (EP) inhibitor in Staphylococcus aureus strains RN-4220 and IS-58, which carry EPs for erythromycin (MrsA) and tetracycline (TetK), respectively.Methods:The minimum inhibitory concentrations (MIC) of limonene and other efflux pump inhibitors were determined through the broth microdilution method. A reduction in the MIC of ethidium bromide was used as a parameter of EP inhibition.Result::While limonene was not shown to exhibit direct antibacterial effects against EP-carrying strains, in association with ethidium bromide and antibiotics, this compound demonstrated enhanced antibacterial activity, indicating the inhibition of the MrsA and TetK pumps.Conclusion::In conclusion, this pioneering study demonstrated the effectiveness of limonene as an EP inhibitor in S. aureus strains, RN-4220 and IS-58. Nevertheless, further studies are required to characterize the molecular mechanisms associated with limonene-mediated EP inhibition.
Liver Damage Produced by Malnutrition is Improved by Dietary Supplementation in Mice: Assessment of a Supplement Based on Buriti (A Cerrado Fruit) and Dairy By-products
Authors: Bicalho Audrey Handyara , do Santos Fabio Ribeiro , Moreira Daniele Cristina , Oliveira Luis Paulo , Machado Amanda Souto , Farias Lucyana , DE Paula Alfredo Mauricio Batista , Guimarães André Luis Sena , Santos Sérgio Henrique Sousa 
Published: January 2021

Background:
Malnutrition induced by dietary restriction produces several metabolic
changes that affect body weight, the digestive system, and annex organs, including the liver. Malnutrition
generates an inflammatory state and increases oxidative stress. The liver is one of the
body vital organs, becoming necessary to analyze the impact of food supplementation on the repair
of possible changes that may occur in this organ due to malnutrition.

Aims:
To evaluate the effects of a low-cost supplementation derived from Buriti and dairy byproducts
on liver recovery in malnourished mice, focusing on the expression of oxidative stressrelated
genes, as well as biochemical and histological parameters.

Methods:
Swiss mice were divided into six groups and submitted to two treatment phases: food restriction,
for malnutrition onset; and renutrition, with mice being fed with different diets.

Results:
Our results indicate that dietary supplementation was successful in recovering liver damage
caused by malnutrition in animal models. The new supplement has been shown to recover liver
damage with similar or superior results compared to the commercial reference supplement on the
market.

Conclusion:
Our work presents a new composition of low cost food supplement based on buriti
and dairy by-products, proven to be effective in the malnutrition treatment of malnutrition. The improvements
were proven through the recovery of body weight, reduction of inflammation and oxidative
stress.
Research on Fog Resource Scheduling based on Cloud-fog Collaboration Technology in the Electric Internet of Things
Authors: Zhu Youchan , Wang Yingzi , Liang Weixuan 
Published: January 2021

Background:
With the further development of the electric Internet of Things (eIoT), IoT
devices in the distributed network generate data with different frequencies and types.

Objective:
Fog platform is located between the smart collected terminal and cloud platform, and the
resources of fog computing are limited, which affects the delay of service processing time and response
time.

Methods:
In this paper, an algorithm of fog resource scheduling and load balancing is proposed.
First, the fog devices divide the tasks into high or low priority. Then, the fog management nodes
cluster the fog nodes through the K-mean+ algorithm and implement the earliest deadline first
dynamic (EDFD) task scheduling algorithm and De-REF neural network load balancing algorithm.

Results:
We use tools to simulate the environment, and the results show that this method has strong
advantages in -30% response time, -50% scheduling time, delay, -50% load balancing rate, and energy
consumption, which provides a better guarantee for eIoT.

Conclusion:
Resource scheduling is an important factor affecting system performance. This article
mainly addresses the needs of eIoT in terminal network communication delay, connection failure,
and resource shortage. A new method of resource scheduling and load balancing is proposed. The
evaluation was performed, and it proved that our proposed algorithm has better performance than
the previous method, which brings new opportunities for the realization of eIoT.
Thermochemical Characterization of Rice Husk (Oryza Sativa Linn) for Power Generation
Authors: Radenahmad Nikdalila , Reza Md Sumon , Bakar Muhammad S. Abu , Azad Abul K. 
Published: January 2021

Rice husk is biomass that can be utilized as fuel for biomass gasification as a renewable energy source. In this paper, thermochemical methods were used to determine the higher heating values, moisture content, bulk density, pellet density, microstructure, and elemental composition of Thai Rice Husk (Oryza Sativa Linn). The heating energy was analyzed using a bomb calorimeter, which showed a higher heating value of 15.46 MJ/kg. Determination of pellet density through rice husk powder pelletization exhibited a value of 1.028 g/cm3, while moisture content was 5.017 wt%. The heating value and moisture content revealed good agreement with the literature values, indicating the potentiality of rice hush for energy generation. Scanning electron microscopy (SEM) showed that the raw rice husk and its ash have similar porosity types but different bulk structure. Elemental analysis using energy dispersive X-ray (EDX) indicated that rice husk contains O, Si, C while O and C percentages were drastically decreased during combustion. The obtained heating value and moisture content proved that rice husk could be used as a bio-energy source in biomass gasification for power generation.