bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D-printed graphene/polymer structures for electron-tunneling based devices
Authors: Carvalho Fernandes Deisy C. , Lynch Dylan , Berry Vikas 
Published: July 2020

AbstractDesigning 3D printed micro-architectures using electronic materials with well-understood electronic transport within such structures will potentially lead to accessible device fabrication for ‘on-demand’ applications. Here we show controlled nozzle-extrusion based 3D printing of a commercially available nano-composite of graphene/polylactic acid, enabling the fabrication of a tensile gauge functioning via the readjustment of the electron-tunneling barrier width between conductive graphene-centers. The electronic transport in the graphene/polymer 3D printed structure exhibited the Fowler Nordheim mechanism with a tunneling width of 0.79–0.95 nm and graphene centers having a carrier concentration of 2.66 × 1012/cm2. Furthermore, a mechanical strain that increases the electron-tunneling width between graphene nanostructures (~ 38 nm) by only 0.19 Ǻ reduces the electron flux by 1e/s/nm2(from 18.51 to 19.51 e/s/nm2) through the polylactic acid junctions in the 3D-printed heterostructure. This corresponds to a sensitivity of 2.59 Ω/Ω%, which compares well with other tensile gauges. We envision that the proposed electron-tunneling model for conductive 3D-printed structures with thermal expansion and external strain will lead to an evolution in the design of next-generation of ‘on-demand’ printed electronic and electromechanical devices.
An analysis of analgesia and opioid prescribing for veterans after thoracic surgery
Authors: Pommerening Matthew J. , Landau Aaron , Hrebinko Katherine , Luketich James D. , Dhupar Rajeev 
Published: July 2020

AbstractThe opioid crisis is a public health issue and has been linked to physician overprescribing. Pain management after thoracic surgery is not standardized at many centers, and we hypothesized that excessive narcotics were being dispensed on discharge. As a quality improvement initiative, we sought to understand current prescribing practices to better align the amount of opioids dispensed on discharge to actual patient needs. This was a single-center, retrospective review of patients undergoing thoracic surgery from 7/2015 to 7/2018. Demographics, operative data, perioperative pain medication use, and discharge pain medication prescriptions were analyzed. Opioids were converted to Morphine Milligram Equivalents (MME). Among 124 patients, 103 (83%) received intraoperative nerve blocks and 106 (85.5%) used PCAs. Prescribed MME/day at discharge were significantly higher than MME/day received during hospitalization (Median 30 [IQR 30–45] vs. 15 [IQR 5–24], p < 0.001) and were not associated with receiving a nerve block or PCA. By procedure, prescribed MME/day were significantly higher than inpatient MME/day for wedge resections (p < 0.001), segmentectomies (p = 0.02), lobectomies (p = 0.003), and thymectomies (p = 0.02). Patients are being discharged with significantly more opioids than they are using as inpatients. Education among prescribers and a standardized approach with patient-specific dosing may reduce excessive opioid dispensing.
Enriched environments enhance cognition, exploratory behaviour and brain physiological functions of Sparus aurata
Authors: Arechavala-Lopez P. , Caballero-Froilán J. C. , Jiménez-García M. , Capó X. , Tejada S. , Saraiva J. L. , Sureda A. , Moranta D. 
Published: July 2020

AbstractEnvironmental enrichment is considered as a recommended tool to guarantee or improve the welfare of captive fish. This study demonstrates for the first time that structural environmental enrichment enhances cognition, exploratory behaviour and brain physiological functions of gilthead seabream (Sparus aurata). Seabream was reared in groups (n = 15) during 60 days under two different treatments: enriched tanks with plant-fibre ropes (EE) or bare/non-enriched tanks (NE). Fish were then exposed to a purpose-built maze for 1 h every second day in four trials. Analysis of video recordings showed that seabream under EE conditions presented higher overall exploratory behaviour, spatial orientation and learning capability compared to seabream from NE conditions. Results from brain monoamines analyses may suggest increased recent dopaminergic activity in telencephalon, known to be involved in learning processes; and increased serotonergic activity in cerebellum, involved in the coordination of balance, movements and orientation. In addition, EE-reared fish showed increased antioxidant activity in whole brain, with no apparent oxidative damage. Structural EE seemed to induce an hormetic response on juvenile seabream, improving their welfare status during captivity. Application of this kind of physical structure might be feasible at fish farms as a passive and non-invasive tool to improve welfare of intensively cultured seabream.
A model-based analysis of phenytoin and carbamazepine toxicity treatment using binding-competition during hemodialysis
Authors: Maheshwari Vaibhav , Hoffman Robert S. , Thijssen Stephan , Tao Xia , Fuertinger Doris H. , Kotanko Peter 
Published: July 2020

AbstractHemodialysis (HD) has limited efficacy towards treatment of drug toxicity due to strong drug-protein binding. In this work, we propose to infuse a competitor drug into the extracorporeal circuit that increases the free fraction of a toxic drug and thereby increases its dialytic removal. We used a mechanistic model to assess the removal of phenytoin and carbamazepine during HD with or without binding-competition. We simulated dialytic removal of (1) phenytoin, initial concentration 70 mg/L, using 2000 mg aspirin, (2) carbamazepine, initial concentration 35 mg/L, using 800 mg ibuprofen, in a 70 kg patient. The competitor drug was infused at constant rate. For phenytoin (~ 13% free at t = 0), HD brings the patient to therapeutic concentration in 460 min while aspirin infusion reduces that time to 330 min. For carbamazepine (~ 27% free at t = 0), the ibuprofen infusion reduces the HD time to reach therapeutic concentration from 265 to 220 min. Competitor drugs with longer half-life further reduce the HD time. Binding-competition during HD is a potential treatment for drug toxicities for which current recommendations exclude HD due to strong drug-protein binding. We show clinically meaningful reductions in the treatment time necessary to achieve non-toxic concentrations in patients poisoned with these two prescription drugs.
Transcriptional and in silico analyses of MIF cytokine and TLR signalling interplay in the LPS inflammatory response of Ciona robusta
Authors: Arizza Vincenzo , Bonura Angela , La Paglia Laura , Urso Alfonso , Pinsino Annalisa , Vizzini Aiti 
Published: July 2020

AbstractThe close phylogenetic relationship betweenCiona robustaand vertebrates makes it a powerful model for studying innate immunity and the evolution of immune genes. To elucidate the nature and dynamics of the immune response, the molecular mechanisms by which bacterial infection is detected and translated into inflammation and how potential pattern recognition receptors (PRRs) are involved in pathogen recognition in tunicateC. robusta(formerly known asCiona intestinalis), we applied an approach combining bacterial infections, next-generation sequencing, qRT-PCR, bioinformatics and in silico analyses (criteria of a p-value < 0.05 and FDR < 0.05). A STRING analysis indicated a functional link between components of the Tlr/MyD88-dependent signalling pathway (Tlr2, MyD88, and Irak4) and components of the Nf-κB signalling pathway (Nf-κB, IκBα, and Ikkα) (p-value < 0.05, FDR < 0.05). A qRT-PCR analysis of immune genes selected from transcriptome data revealedMifas more frequently expressed in the inflammatory response than inflammation mediator or effector molecules (e.g., Il-17s, Tnf-α, Tgf-β, Mmp9, Tlrs, MyD88, Irak4, Nf-κB, and galectins), suggesting close interplay between Mif cytokines and Nf-κB signalling pathway components in the biphasic activation of the inflammatory response. An in silico analyses of the 3′-UTR ofTlr2, MyD88, IκBα, Ikk, andNf-κBtranscripts showed the presence of GAIT elements, which are known to play key roles in the regulation of immune gene-specific translation in humans. These findings provide a new level of understanding of the mechanisms involved in the regulation of theC. robustainflammatory response induced by LPS and suggest that inC. robusta,as in humans, a complex transcriptional and post-transcriptional control mechanism is involved in the regulation of several inflammatory genes.
Impaired iloprost-induced platelet inhibition and phosphoproteome changes in patients with confirmed pseudohypoparathyroidism type Ia, linked to genetic mutations in GNAS
Authors: Swieringa Frauke , Solari Fiorella A. , Pagel Oliver , Beck Florian , Huang Jingnan , Feijge Marion A. H. , Jurk Kerstin , Körver-Keularts Irene M. L. W. , Mattheij Nadine J. A. , Faber Jörg , Pohlenz Joachim , Russo Alexandra , Stumpel Connie T. R. M. , Schrander Dirk E. , Zieger Barbara , van der Meijden Paola E. J. , Zahedi René P. , Sickmann Albert , Heemskerk Johan W. M. 
Published: July 2020

AbstractPatients diagnosed with pseudohypoparathyroidism type Ia (PHP Ia) suffer from hormonal resistance and abnormal postural features, in a condition classified as Albright hereditary osteodystrophy (AHO) syndrome. This syndrome is linked to a maternally inherited mutation in the GNAS complex locus, encoding for the GTPase subunit Gsα. Here, we investigated how platelet phenotype and omics analysis can assist in the often difficult diagnosis. By coupling to the IP receptor, Gsα induces platelet inhibition via adenylyl cyclase and cAMP-dependent protein kinase A (PKA). In platelets from seven patients with suspected AHO, one of the largest cohorts examined, we studied the PKA-induced phenotypic changes. Five patients with a confirmed GNAS mutation, displayed impairments in Gsα-dependent VASP phosphorylation, aggregation, and microfluidic thrombus formation. Analysis of the platelet phosphoproteome revealed 2,516 phosphorylation sites, of which 453 were regulated by Gsα-PKA. Common changes in the patients were: (1) a joint panel of upregulated and downregulated phosphopeptides; (2) overall PKA dependency of the upregulated phosphopeptides; (3) links to key platelet function pathways. In one patient with GNAS mutation, diagnosed as non-AHO, the changes in platelet phosphoproteome were reversed. This combined approach thus revealed multiple phenotypic and molecular biomarkers to assist in the diagnosis of suspected PHP Ia.
DeepGhost: real-time computational ghost imaging via deep learning
Authors: Rizvi Saad , Cao Jie , Zhang Kaiyu , Hao Qun 
Published: July 2020

AbstractThe potential of random pattern based computational ghost imaging (CGI) for real-time applications has been offset by its long image reconstruction time and inefficient reconstruction of complex diverse scenes. To overcome these problems, we propose a fast image reconstruction framework for CGI, called “DeepGhost”, using deep convolutional autoencoder network to achieve real-time imaging at very low sampling rates (10–20%). By transferring prior-knowledge from STL-10 dataset to physical-data driven network, the proposed framework can reconstruct complex unseen targets with high accuracy. The experimental results show that the proposed method outperforms existing deep learning and state-of-the-art compressed sensing methods used for ghost imaging under similar conditions. The proposed method employs deep architecture with fast computation, and tackles the shortcomings of existing schemes i.e., inappropriate architecture, training on limited data under controlled settings, and employing shallow network for fast computation.
Genome-wide identification and characterization of Solanum tuberosum BiP genes reveal the role of the promoter architecture in BiP gene diversity
Authors: Herath Venura , Gayral Mathieu , Adhikari Nirakar , Miller Rita , Verchot Jeanmarie 
Published: July 2020

AbstractThe endoplasmic reticulum (ER) immunoglobulin binding proteins (BiPs) are molecular chaperones involved in normal protein maturation and refolding malformed proteins through the unfolded protein response (UPR). Plant BiPs belong to a multi-gene family contributing to development, immunity, and responses to environmental stresses. This study identified three BiP homologs in the Solanum tuberosum (potato) genome using phylogenetic, amino acid sequence, 3-D protein modeling, and gene structure analysis. These analyses revealed that StBiP1 and StBiP2 grouped with AtBiP2, whereas StBiP3 grouped with AtBiP3. While the protein sequences and folding structures are highly similar, these StBiPs are distinguishable by their expression patterns in different tissues and in response to environmental stressors such as treatment with heat, chemicals, or virus elicitors of UPR. Ab initio promoter analysis revealed that potato and Arabidopsis BiP1 and BiP2 promoters were highly enriched with cis-regulatory elements (CREs) linked to developmental processes, whereas BiP3 promoters were enriched with stress related CREs. The frequency and linear distribution of these CREs produced two phylogenetic branches that further resolve the groups identified through gene phylogeny and exon/intron phase analysis. These data reveal that the CRE architecture of BiP promoters potentially define their spatio-temporal expression patterns under developmental and stress related cues.
Predicting and comparing postoperative infections in different stratification following PCNL based on nomograms
Authors: Jiang Enyan , Guo Haixiang , Yang Bowei , Li Pei , Mishra Prashant , Yang Tongxin , Li Yuhang , Wang Haifeng , Jiang Yongming 
Published: July 2020

AbstractTo discuss the mechanisms of infection complications in different degrees after percutaneous nephrolithotomy (PCNL) through predicting and comparing post-PCNL infections based on nomograms, a retrospective cohort study was conducted among 969 cases who underwent PCNL from Dec 5, 2016 to Dec 25, 2017 in Kunming, Yunnan Province. We examined clinical features, urine routine, blood routine, blood biochemistry, imaging studies and operative information and recorded the examination results before surgery for univariate and multivariate logistic regression. We applied receiver operating characteristic curves, calibration curves, accuracy, specificity, sensitivity, positive predictive value and negative predictive value to evaluate and compare the models. Nomograms were used to visualize the different degrees of postoperative infection complications. The risk scores of the three groups were compared by diabetes mellitus distribution. Our results suggest that the more severe the infection is, the more accurate the model predicts and that the occurrence of severe infection mostly is related to the patients' homeostasis. Hence, we developed an online post-PCNL sepsis dynamic nomogram which can achieve visualization and dynamically predict the incidence of sepsis in postoperative patients.
A long noncoding RNACHAIRprotects the heart from pathological stress
Authors: Qian Yanxia , Zhang Mingming , Zhou Ningtian , Xu Xiaohan , Zhang Jiahui , Ding Qiang , Wang Junhong 
Published: July 2020

AbstractMammalian genomes have been found to be extensively transcribed. In addition to classic protein coding genes, a large numbers of long noncoding genes (lncRNAs) have been identified, while their functions, especially in heart diseases, remain to be established. We hypothesized that heart failure progression is controlled by tissue-specific lncRNAs. In the present study, we found that the cardiac-enriched lncRNA 4632428C04Rik, named as cardiomyocyte hypertrophic associated inhibitory RNA (CHAIR), is dynamically regulated during heart development, is expressed at low levels in embryonic hearts and accumulated at high levels in adult hearts. More interestingly, the lncRNA was down-regulated during cardiac hypertrophy and failure both in mice and humans. Importantly, loss of lncRNA CHAIR has no effects on normal hearts, whereas it results in accelerated heart function decline, increased hypertrophy, and exacerbated heart failure in response to stress. In contrast, restoring the expression of lncRNA CHAIR rescued the hearts from hypertrophy and failure. DNMT3A was recruited to CHAIR promoter during heart failure to suppress its expression. Reciprocally, CHAIR interacted with DNMT3A to inhibit its DNA-binding activity. Taken together, our data revealed a new cardioprotective lncRNA that represses heart failure through an epigenetic mechanism.
Distale Bizepssehnen- und Trizepssehnenrupturen
Authors: Freislederer Florian , Papillo Donato , Glanzmann Michael , Scheibel Markus 
Published: July 2020

ZusammenfassungRupturen der distalen Bizeps- und Trizepssehne sind selten, treten jedoch gehäuft bei männlichen, sportlichen und körperlich schwer arbeitenden Patienten auf. Dieser Übersichtsartikel beschreibt Ätiologie und Pathogenese sowie Diagnostik, Behandlungsmöglichkeiten, das zu erwartende Outcome dieser Verletzungen und gibt Einblicke in das eigene Vorgehen.
Molecular Identification Based on Chloroplast Sequences and Anti-complementary Activity Comparison of Juniperus Samples from the Qinghai-Tibet Plateau
Authors: Yang Yi-Ting , De Ji , Fu Ze-Long , Wang Xie-Li , Chen Dao-Feng , Xie Hui , Lu Yan 
Published: July 2020

Abstract
Juniperus (Cupressaceae, Pinales) plants are widely distributed in the Qinghai-Tibet Plateau of China. The leaves and twigs of at least 8 Juniperus species (J. pingii, J. pingii var. wilsonii, J. squamata, J. recurva var. coxii, J. saltuaria, J. indica, J. tibetica and J. convallium var. microsperma) have been used as the Tibetan medicine Xuba. At present, it is difficult to distinguish among the original species of Xuba based only on their similar morphological characteristics. However, in our previous studies, 4 Xuba samples from different Juniperus species exhibited significant differences in both anticomplementary activity in vitro and anti-inflammatory effects on acute lung injury in vivo. To identify the effective original species of Xuba reliably, in this study, we developed a sequencing-based DNA molecular technology to distinguish 14 populations of 8
Juniperus species collected from Tibet region, using trnS-G, trnD – T, and petN-psbM genomic regions to build phylogenetic trees. In addition, their anticomplementary activities were evaluated. The results showed that combined sequence of these 3 genomic regions could identify 8 Juniperus species clearly and clustered individuals of one species but from different locations, whichever phylogenetic tree was constructed. Moreover, the anticomplementary activities of the 8 species were clustered into 2 groups. Among them, J. saltuaria and J. recurva var. coxii, which formed an independent branch apart from the other 6 species in phylogenetic trees, were the most potent (CH50: 0.029 – 0.032 mg/mL). Consequently, DNA identification of Juniperus using the combined sequence could provide beneficial guidance for further efficacy evaluation and quality control of Xuba.
Syzygium aromaticum Reduces Diabetes-induced Glucotoxicity via the NRF2/Glo1 Pathway
Authors: Do Moon Ho , Choi Jiwon , Kim Yoonsook , Ha Sang Keun , Yoo Guijae , Hur Jinyoung 
Published: July 2020

AbstractAdvanced glycation end products and methylglyoxal are known to show increased levels in diabetic conditions and induce diverse metabolic disorders. However, the antiglycation ability of the bark of Syzygium aromaticum is not yet studied. In this study, we determined the inhibitory effects of S. aromaticum on AGE formation. Moreover, S. aromaticum showed breakage and inhibitory ability against the formation of AGE-collagen crosslinks. In SV40 MES13 cells, treatment with the S. aromaticum extract significantly ameliorated MG-induced oxidative stress as well as cytotoxicity. Furthermore, in the S. aromaticum extract-treated group, there was a reduction in levels of several diabetic markers, such as blood glucose, kidney weight, and urinary albumin to creatinine ratio in streptozotocin-induced diabetic rats. Treatment with the S. aromaticum extract significantly increased the expression of nuclear factor erythroid 2-related factor 2, a transcription factor involved in the expression of antioxidant enzymes. Moreover, the treatment significantly upregulated the expression of glyoxalase 1 and downregulated the expression of receptor for AGEs. These results suggest that the S. aromaticum extract might ameliorate diabetes-induced renal damage by inhibiting the AGE-induced glucotoxicity and oxidative stress through the Nrf2/Glo1 pathway.
Non-planar Boron Lewis Acids Taking the Next Step: Development of Tunable Lewis Acids, Lewis Superacids and Bifunctional Catalysts
Authors: Berionni Guillaume , Chardon Aurélien , Osi Arnaud , Mahaut Damien , Saida Ali Ben 
Published: July 2020

Although boron Lewis acids commonly adopt a trigonal planar geometry, a number of compounds in which the trivalent boron atom is located in a pyramidal environment have been described. This review will highlight the recent developments of the chemistry and applications of non-planar boron Lewis acids, including a series of non-planar triarylboranes derived from the triptycene core. A thorough analysis of the properties and of the influence of the pyramidalization of boron Lewis acids on their stereoelectronic properties and reactivities is presented based on recent theoretical and experimental studies.1	Non-planar Trialkylboranes2	Non-planar Alkyl and Aryl-Boronates3	Non-planar Triarylboranes and Alkenylboranes3.1	Previous Investigations on Bora Barrelenes and Triptycenes3.2	Recent Work on Boratriptycenes from Our Research Group4	Applications of Non-planar Boranes4.1	Non-planar Alkyl Boranes and Boronates4.2	Non-planar Triarylboranes (Boratriptycenes)5	Other Non-planar Group 13 Lewis Acids6	Further Work and Perspectives