bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiplexed mRNA assembly into ribonucleoprotein particles plays an operon-like role in the control of yeast cell physiology
Authors: Nair Rohini R. , Zabezhinsky Dimitry , Gelin-Licht Rita , Haas Brian , Dyhr Michael C.A. , Sperber Hannah S. , Nusbaum Chad , Gerst Jeffrey E. 
Published: June 2020

AbstractProkaryotes utilize polycistronic messages (operons) to co-translate proteins involved in the same biological process. Whether eukaryotes achieve similar regulation by selectively assembling monocistronic messages derived from different chromosomes is unclear. We employed transcript-specific RNA pulldowns and RNA-seq/RT-PCR to identify mRNAs that co-precipitate into ribonucleoprotein (RNP) particles in yeast. Consistent with the hypothesis of eukaryotic RNA operons, mRNAs encoding components of the mating pathway, heat shock proteins, and mitochondrial outer membrane proteins multiplexin transto form discrete mRNP particles, termed transperons. Chromatin-capture experiments reveal that genes encoding multiplexed mRNAs physically interact, thus, RNA assembly may result from co-regulated gene expression. Transperon assembly and function depends upon H4 histones and their depletion leads to defects in RNA multiplexing, resulting in decreased pheromone responsiveness and mating, and increased heat shock sensitivity. We propose that intergenic associations and non-canonical H4 histone functions contribute to transperon formation in eukaryotic cells to regulate cell physiology.Abstract Figure
The language of mental images: Characterizing hippocampal contributions to imageable word use during event construction
Authors: Race Elizabeth , Carlisle Camille , Tejwani Ruchi , Verfaellie Mieke 
Published: June 2020

AbstractAccumulating evidence suggests that the hippocampus plays a critical role in the creative and flexible use of language. For example, amnesic patients with hippocampal damage produce less coherent and cohesive verbal discourse when constructing narratives about the past, present, and future. A recent study by Hilverman and colleagues (2017) found that amnesic patients with hippocampal damage also use less imageable words during narrative construction compared to healthy controls. These results suggest that in addition to supporting language use at the discourse level, the hippocampus also influences the quality of language at the single word level. However, the generalizability of these results to different types of language production tasks and the relationship to patients’ broader impairments in episodic memory have yet to be examined. In the current study, we investigated whether amnesic patients with hippocampal damage produce less imageable words compared to healthy controls in two different types of language production tasks. In Experiment 1, participants constructed narratives about events depicted in visually presented pictures (picture narratives). In Experiment 2, participants constructed verbal narratives about remembered events from the past or simulated events in the future (past/future narratives). Across all types of narratives, patients produced words that were rated as having similar levels of imageability compared to controls. Importantly, this was the case both in patients’ picture narratives, which did not require generating details from long-term memory and were matched to controls’ with respect to narrative content, and in patients’ narratives about past/future events, which required generating details from long-term memory and which were reduced in narrative content compared to those of controls. These results reveal that the hippocampus is not necessary for the use of imageable representations at the linguistic level, and that hippocampal contributions to imageable word use are independent of hippocampal contributions to episodic memory.
SARS-CoV-2 & Pediatric Mental Health: A Review of Recent Evidence
Authors: Sajid Mir Ibrahim , Tariq Javeria , Waheed Ayesha Akbar , Dur-e-Najaf  , Balouch Samira Shabbir , Abaidullah Sajid 
Published: June 2020

ABSTRACTSARS-CoV-2 was declared a global pandemic by the World Health Organization (WHO) and was met with lockdown policies to curb the spread of the disease. This meant that 890 million students in 114 countries would be affected by the closure of their educational institutes, affecting their mental health. Mental health disorders are suggested to have a well-correlated link to suicide, which is the third most leading cause of death worldwide amongst children aged 15-19 years. According to WHO, “health is a state of complete physical, mental and social wellbeing and not merely the absence of disease.” Hence the isolation brought about by SARS-CoV-2 is postulated to cause anxiety, fear, and depression amongst the pediatric population, due to the loss of socialization and separation from friends. In this systematic review and meta-analysis, we highlight the major mental health issues in children aged 2-18 years, along with their causes, effects, and potential solutions to tackle these problems.
Predictive model of COVID-19 incidence and socioeconomic description of municipalities in Brazil
Authors: Carneiro Isadora C. R. , Ferreira Eloiza D. , Silva Janaina C. da , Soares Guilherme , Strottmann Daisy M. , Silveira Guilherme F. 
Published: June 2020

ABSTRACTCoronaviruses are enveloped viruses that can cause respiratory, gastrointestinal, hepatic, and neurological diseases. In December 2019, a new highly contagious coronavirus termed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China. SARS-CoV-2 causes a potentially lethal human respiratory infection, COVID-19, that is associated with fever and cough and can progress to pneumonia and dyspnea in severe cases. Since the virus emerged, it has spread rapidly, reaching all continents around the world. A previous study has shown that, despite being the best alternative in the current pandemic context, social distancing measures alone may not be sufficient to prevent COVID-19 spread, and the overall impact of the virus is of great concern. The present study aims to describe the demographic and socioeconomic characteristics of 672 cities with cases of COVID-19, as well as to determine a predictive model for the number of cases. We analyzed data from cities with at least 1 reported case of COVID-19 until June 26, 2020. It was observed that cities with confirmed cases of the disease are present in all Brazilian states, affecting 36.5% of the municipalities in Rio de Janeiro State. The inhabitants in cities with reported cases of COVID-19 represent more than 73.1% of the Brazilian population. Stratifying the age groups of the inhabitants and accounting for the percentage of women and men does not affect COVID-19 incidence (confirmed cases/100,000 inhabitants). The demographic density, the MHDI and the per capita income of the municipalities with cases of COVID-19 do not affect disease incidence. In addition, if conditions are maintained, our model predicts 2,358,703 (2,172,930 to 2,544,477) cumulative cases on July 25, 2020.
COVID-19: Saving lives and livelihoods using population density driven testing
Authors: Budhwani Karim I. , Budhwani Henna , Podbielski Ben 
Published: June 2020

AbstractSARS-CoV-2 transmission risk generally increases with proximity of those shedding the virus to those susceptible to infection. Thus, this risk is a function of both number of people and the area which they occupy. However, the latter continues to evade COVID-19 testing policy. Increased testing in areas with lower population density, has the potential to induce a false sense of security even as cases continue to rise sharply overall.
Seroconversion of a city: Longitudinal monitoring of SARS-CoV-2 seroprevalence in New York City
Authors: Stadlbauer Daniel , Tan Jessica , Jiang Kaijun , Hernandez Matthew M. , Fabre Shelcie , Amanat Fatima , Teo Catherine , Arunkumar Guha Asthagiri , McMahon Meagan , Jhang Jeffrey , Nowak Michael D. , Simon Viviana , Sordillo Emilia Mia , Bakel Harm van , Krammer Florian 
Published: June 2020

AbstractBy conducting a retrospective, cross-sectional analysis of SARS-CoV-2 seroprevalence in a ‘sentinel group’ (enriched for SARS-CoV-2 infections) and a ‘screening group’ (representative of the general population) using >5,000 plasma samples from patients at Mount Sinai Hospital in New York City (NYC), we identified seropositive samples as early as in the week ending February 23, 2020. A stark increase in seropositivity in the sentinel group started the week ending March 22 and in the screening group in the week ending March 29. By the week ending April 19, the seroprevalence in the screening group reached 19.3%, which is well below the estimated 67% needed to achieve community immunity to SARS-CoV-2. These data potentially suggest an earlier than previously documented introduction of SARS-CoV-2 into the NYC metropolitan area.One Sentence SummarySeroprevalence of SARS-CoV-2 in cross-sectional samples from New York City rose from 0% to 19.3% from early February to mid-April.
Circulating tumor DNA dynamics using a standardized multi-gene panel in advanced breast cancer treated with CDK4/6 inhibition and endocrine therapy
Authors: Martínez-Sáez Olga , Pascual Tomás , Brasó-Maristany Fara , Chic Nuria , González-Farré Blanca , Sanfeliu Esther , Rodríguez Adela , Martínez Débora , Galván Patricia , Belén Rodríguez Anna , Schettini Francesco , Conte Benedetta , Vidal Maria , Adamo Barbara , Martinez Antoni , Muñoz Montserrat , Moreno Reinaldo , Villagrasa Patricia , Salvador Fernando , Ciruelos Eva M. , Faull Iris , Odegaard Justin I. , Prat Aleix 
Published: June 2020

Brief communicationCirculating tumor DNA (ctDNA) levels may predict response to anticancer drugs1, including CDK4/6 inhibitors and endocrine therapy combinations (CDK4/6i+ET)2; however, critical questions remain unanswered such as which assay or statistical method to use3. Here, we obtained paired plasma samples at baseline and week 4 in 45 consecutive patients with advanced breast cancer treated with CDK4/6i+ET. ctDNA was detected in 96% of cases using the 74-gene Guardant360 assay4,5. A variant allele fraction ratio (VAFR) was calculated for each of the 79 detected mutations between both time-points. Mean of all VAFRs (mVAFR) was computed for each patient. In our dataset, mVAFR was significantly associated with progression-free survival (PFS). Baseline VAF, on-treatment VAF or absolute changes in VAF were not associated with PFS. These findings demonstrate that ctDNA dynamics using a standardized multi-gene panel and a unique methodological approach predicts treatment outcome. Clinical trials in patients with an unfavorable ctDNA response are needed.
Design and tests of a non-contact Bernoulli gripper for rough-surfaced and fragile objects gripping
Authors: Liu Dong , Wang Minghao , Fang Naiyu , Cong Ming , Du Yu 
Published: June 2020

Purpose
Varied shapes and sizes of different products with irregular rough surface and fragile properties give a challenge to traditional contact gripping. Single Bernoulli grippers are not suited to handle fragile objects as the impact of center negative pressure force could result in large deformation and stress which damage the materials, and they are also have some limitations for gripping objects with different large and small shapes. Thus, this paper aims to design a non-contact gripper for soft, rough-surfaced and fragile objects gripping with multi Bernoulli heads, which have optimal structures and parameters.

Design/methodology/approach
The compressed air is ejected into four Bernoulli heads through radial and long flow channels, then passes through four strip-shaped narrow gaps after fully developing in the annular cavity to provide negative pressure. Based on the mathematic model and the computational model, the key structural parameters affecting the gripping performance are selected, and parameters optimization of the gripper is performed by computational fluid dynamics simulation analysis and performance evaluation. The orthogonal method is used and L16 orthogonal array is selected for experimental design and optimization. The characteristics of the designed gripper are tested from the aspects of pressure distribution and lifting force.

Findings
From the applications in gripping different objects, the designed non-contact gripper can grip varied shapes and sizes of soft, rough-surfaced, fragile and sliced objects with little effect of torque.

Originality/value
In this paper, a non-contact gripper is designed for handling soft, rough-surfaced and fragile objects based on the Bernoulli principle. A systematic approach, which consists of modeling, simulation, optimization and measurement is provided for the non-contact gripper design and tests.
Rake impact on turboshaft compressors, a numerical study
Authors: Dragan Valeriu , Dumitrescu Oana , Malael Ion , Azoitei Adrian Daniel 
Published: June 2020

Purpose
Turboshaft engines usually include one centrifugal compressor due to its high-pressure ratio, stability and compactness. Many designers rely on positive raking to decrease tip gap flow and therefore losses. However recent optimization studies revealed geometries contradicting this canonic view. Hence, this paper aims to investigate how the rake angle alone can influence performance and to which extent.

Design/methodology/approach
A turboshaft representative impeller was chosen and altered for null and +/−30° rake angles. Menter's shear stress transport model is used for steady computational fluid dynamics simulations, sweeping the nominal speedline at various tip clearances. Backsweep distribution is identical in all cases, isolating rake influence.

Findings
Pressure ratio was lowered for the both positively and negatively raked blades, but through distinct aerodynamic mechanisms. Although the flow through the tip gap was lower for the positive rake, this is due to lower blade loading. Splitter comparison reveal that these effects are more pronounced in the radial regions.

Practical implications
Some of the findings may extend beyond turboshaft engines, into turbochargers, home appliances or industrial blowers. However, all extrapolations must consider specific differences between these applications. Turboshaft compressors designers can benefit from this study when setting up their free parameters and penalty functions in the early concept stages.

Originality/value
Only few similar studies can be found in the literature to date, none similar to turboshaft applications. Also, this impeller is designed to eliminate leading edge shocks and suction side boundary layer separation, which makes it easier to isolate the tip gap flow effects. The authors also provide a framework on which semi-empirical design equations can be further developed to incorporate rake into 1D design tools.
Consumer acceptance of cultured meat: some hints from Italy
Authors: Palmieri Nadia , Perito Maria Angela , Lupi Claudio 
Published: June 2020

PurposeThe purpose of this paper is to contribute to the current literature on consumer acceptance of cultured meat and to investigate the main factors that might affect it.Design/methodology/approachData were collected from a sample of 490 consumers in Italy, using a web-based survey. The empirical analysis follows an exploratory approach based on the training and checking of a random forest model.FindingsAn important finding of this study concerns the overall positive perception of cultured meat on the part of the interviewees in a country that is the fifth-largest meat producer at the European level. Age, environmental and ethical issues, and scepticism about new food technologies are the most important factors that guide consumer acceptance of cultured meat. The results suggest that in order to increase cultured meat acceptance it would be important to inform and educate consumers towards new food and new food production methods.Research limitations/implicationsThe sample analysed in this study is not representative of the whole national population, as it happens in most papers dealing with new food.Originality/valueAlthough the conclusions of this exploratory study cannot be over-generalized, the results provide interesting insights on how to increase cultured meat acceptance in view of the possible development of a new market for cultured meat.
School performance in three South East Asian countries: lessons in leadership, decision-making and training
Authors: Gill CM Hugues D. , Berezina Elizaveta 
Published: June 2020

Purpose
The three neighbouring nations of Indonesia, Malaysia and Singapore participated in the 2009, 2012 and 2015 Programme for International Student Assessment (PISA) cycles. Despite many similarities between the three nations, Singapore has consistently been a top PISA performer, with Malaysia and Indonesia in the bottom third of the international league tables. This paper aims to sketch the comparative Association of South East Asian Nations (ASEAN) context and uses PISA-derived metrics to contrast how differences in decision-making and school leadership, particularly in relation to staff development and training practices, may impact school performance across Indonesia, Malaysia and Singapore.

Design/methodology/approach
Ten scales from the 2015 PISA School Questionnaire for Malaysia, Singapore and Indonesia were analysed using ANOVA and t-tests as an aid to exploring the extent to which different approaches to teacher training, school leadership and governance may impact student performance.

Findings
Although Malaysian and Indonesian school principals report higher levels of autonomy than Singaporean peers, other evidence suggests that schools in Singapore may actually have greater decision latitude. Most significantly, Singaporean teachers take responsibility for key staff development decisions and skills transfer, whereas in Indonesia and Malaysia, teacher training is controlled by government administrators, a factor that may be a critical differentiator between the school systems.

Practical implications
In Singapore, teacher training is controlled by and for teachers through professional learning teams within schools and professional learning communities across schools; in Malaysia and Indonesia, similar decisions are taken by external administrators. Giving Malaysian and Indonesian teachers control over their own training could be a simple and powerful reform to target skills gaps and to generalise improvements in pedagogy quickly across schools and thus to lift school performance in these countries.

Originality/value
This paper highlights how differences at systemic and school levels, particularly in approaches to teacher training and leadership and may explain differentials in school performance in three ASEAN education systems.
Enhancing professional growth and the learning and development function through reflective practices: an autoethnographic narrative approach
Authors: Koukpaki Adebayo Serge Francois , Adams Kweku 
Published: June 2020

Purpose
The purpose of this paper is to explore ways in which learning and development (L&D) professionals use reflective practice to promote the function of L&D and their own professional growth. The claim that L&D practitioners need to develop their reflective ability to make sense of their own practice is well-argued in the literature, but few studies focus on an in-depth individual self-reflection and its impact on professional growth.

Design/methodology/approach
An autoethnography and reflective practice design was deployed. The data was collected by sending a semi-structured, pre-set question as a ‘reflective conversation’ to an L&D manager and a 10-hour tape recording of personal reflection over three months.[AQ1] Data was sanitised, transcribed and edited, and a narrative data analysis method was used to analyse the data developed into reflective narratives.

Findings
The authors find that reflective practice emerges through gradual reflective patterns that define the circumstances surrounding the reflection, the content, exploration and interpretation and confirming the fulfilling of the reflection.

Originality/value
This paper offers the journey of an L&D manager working in the hotel industry in India. Through a set of reflective practices, including introspection and reflexivity, the manager considers the changes she has experienced. The paper contributes to the literature on reflective practice based on promoting the L&D function as an essential part of the horizontal integration of human resource management in organisations. Theoretical and practical implications are discussed.
Entrepreneurship education as an arena for career reflection: the shift of students' career preferences after a business planning course
Authors: Longva Kjersti Kjos , Strand Øivind , Pasquine Mark 
Published: June 2020

PurposeThe purpose of this paper is to examine the impact of entrepreneurship education (EE) in the form of a business planning course on students' career intentions and preferences. While there is extensive research in which traditional survey scales have been applied to study students' entrepreneurial intentions, this study takes a novel approach by extending the construct of entrepreneurial intention to include preferences for intrapreneurship and team entrepreneurship. Furthermore, the use of conjoint analysis captures students' unconscious decision-making processes when presented with different career opportunity scenarios, thereby overcoming many of the limitations of self-reported survey measures.Design/methodology/approachThe study uses a quasi-experimental design with a novel application of conjoint analysis in EE research. A two-part survey combining a traditional questionnaire with conjoint analysis was distributed to students enrolled in a business planning course at two campuses of a Norwegian university, resulting in 99 matched pre- and post-test responses.FindingsTwo main findings arise from the study. First, there is a significant decrease in entrepreneurial intention among students in the EE course. Second, the conjoint analysis contributes to a better understanding of this decrease by illustrating how students shift their career preferences from entrepreneurship to employment during the EE course. This suggests that EE provides a space for students' career reflections where they can explore, commit to and reconsider entrepreneurship as a career.Research limitations/implicationsA limitation of the study is that it focuses on a small sample of undergraduate students from two campus locations in Norway. Thus, further investigation is still necessary to establish whether the findings are valid in other contexts. The research has implications for higher educational institutions, policymakers and researchers in the field of EE.Practical implicationsThe study contributes with a novel perspective on EE as a trigger for career reflection, a perspective that is important for educators teaching EE courses, as well as for higher education institutions who decide to implement EE in study programmes.Originality/valueBy focusing on the development of students' career preferences through conjoint analysis, the study expands knowledge on the impact of EE on students' careers, while also accentuating the value of the application of conjoint analysis in research on EE.