bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system
Authors: Cai Xianghai , Lin Lin , Shen Yaling , wei wei , Wei Dong-zhi 
Published: April 2020

Abstract
Background: Esterases are widely distributed in nature and have important applications in medical, industrial and physiological. Recently, the increased demand for flavor esters has prompted the search of catalysts like lipases and esterases. Esterases from thermophiles also show thermal stability at elevated temperatures and have become enzymes of special interest in biotechnological applications. Although most of esterases catalyzed reactions are carried out in toxic and inflammable organic solvents, the solvent-free system owning many advantages such as low cost and easy downstream processing.Results: The gene estGSU753 from Geobacillus subterraneus DSM13552 was cloned, sequenced and overexpressed into Escherichia coli BL21 (DE3). The novel gene has an open reading frame of 753 bp and encodes 250-amino-acid esterase (EstGSU753). The sequence analysis showed that the protein contains a catalytic triad formed by Ser97, Asp196 and His226, and the Ser of the active site is located in the conserved motif Gly95-X-Ser97-X-Gly99 included in most esterases and lipases. The protein catalyzed the hydrolysis of pNP-esters of different acyl chain lengths, and the enzyme specific activity was 70 U/mg with the optimum substrate pNP-caprylate. The optimum pH and temperature of the recombinant enzyme were 8.0 and 60°C respectively. The resulting EstGSU753 showed remarkable stability against methanol. After the incubation at 50% methanol for 9 days, EstGSU753 retained 50% of its original activity. Even incubation at 90% methanol for 35 h, EstGSU753 retained 50% of its original activity. Also, the preliminary study of the transesterification shows the potential value in synthesis of short-chain flavor esters in a solvent-free system, and more than 99% conversion was obtained in 6 h (substrate: cinnamyl alcohol, 1.0 M).Conclusions: This is the first report of esterase gene cloning from Geobacillus subterraneus with detailed enzymatic properties. This methanol-stable esterase showed potential value in industrial applications especially in the perfume industry.
Monte Carlo Simulation for Cost Forecasting in the Green Building Project
Authors: Muhamad Halil Faridah , Ismail Hafiszah , Hasim Mohamad Sufian , Hashim Halim 
Published: April 2020

Monte Carlo Simulation is a mathematical technique that generates random variables for modelling risk. This technique is suitable and benefits to the various client such as public and private sector to evaluate the costing prepared by the Quantity Surveyor. The methodology used is a qualitative approach consisting of a case study and document analysis. The result shows through Monte Carlo simulation, can predict the worst return from the accuracy of the estimation and given absolute confidence for project development.
Produk Layanan Transaksi Online Studi Pada PT Bank Syariah Mandiri Menurut Perspektif Ekonomi Syariah
Authors: Alimuddin Muhammad , Nurdin Nurdin , Amalia Rizki 
Published: April 2020

Tulisan ini membahas produk layanan transaksi online studi pada PT bank syariah mandiri menurut perspektif ekonomi syariah. Penelitian ini bersifat pendekatan kualitatif, dengan menggunakan teknik dalam pengumpulan data melalui observasi, wawancara dan dokumentasi. Sedangkan sumber data yang di peroleh dari pimpinan cabang dengan penyajian data, reduksi data dan verfikasi data. Hasil penelitian menunjukkan bahwa produk layanan transaksi online (mobile banking) direspon baik oleh nasabah atau pengguna mobile banking karena layanan yang bersifat mempermudah nasabah dalam melakukan transaksi secara online. Disamping itu produk layanan transaksi online banking diberikan BSM kepada nasabah juga agar nasabah bebas melakukan transaksi dimana saja dan kapan saja yang tidak terikat oleh waktu dan tempat. Saran penelitian adalah BSM perlu meningkatkan transaksi secara online yang lebih canggih dan maju untuk kemudahan para pelaku bisnis dan usaha di bidang teknologi dan informasi dalam melakukan transaksi untuk mendapatkan kemaslahatan bagi umut manusia secara umum khususnya di dunia perbankan syariah.
Naturalism and political science: adaptation to the non-classical world picture
Authors: Alekseeva T. A. , Mineyev A. P. 
Published: April 2020

Introduction. One of the greatest achievements of the humanity is obviously the recognition of the systematic unity of the natural and social knowledge. However, this recognition was not constant. The emergence, development and history of political sciences reflected it rather evidently, tending to go from one extreme to another – from identifying its methods with those of positive science to pretending to be unique or even universal. All these questions acquired special importance in the new non-classical world, but the adaptation of political sciences to a new type of thinking meets considerable difficulties.Methods of study. The main method of the study is comparative analysis of the variations to connect philosophical and substantive (ontological and epistemological) tools with political and applied ones of researching political and international political processes and phenomena. Moreover, the authors also used the interpretation approach.Results. The analysis of the most significant approaches towards the political and international processes demonstrate that the acceptance of the new postulates of non-classical and post-nonclassical pictures of the world is quite complicated. Simultaneously with the preservation of the pure mechanistic, approach some of the elements of the new world pictures were taken from quantum physics, biology. The chance factor and the rejection of the casual relationships were also taken into consideration. Nevertheless, it is better to speak not about the transfer of the methods and approaches from natural to political sciences, but about the attempts to build “weak” theories or analogues of theories (for instance, quantum-like theories). Nevertheless, generally speaking, political as well as other social sciences tend to be developing capturing the zeitgeist.Discussion and Conclusions. The adaptation of political sciences to new scientific pictures of the world is inevitable, but limited by definition: for all unity of knowledge as such, their methods and tools are very different and even undergo such significant changes and simplifications in the process of adaptation that they often retain only the names and imitations of the methodologies of other sciences. And yet, at least we have to go in parallel. But some caution here would not hurt at all.
International legal framework for energy cooperation between the member states of the Eurasian Economic Union
Authors: Glikman O. V. , Nazarova A. U. 
Published: April 2020

Introduction. Energy cooperation of the member states of the Eurasian Economic Union plays a crucial role in the framework of the Eurasian economic integration. The EAEU members set as their goal the creation of common energy markets of the Union by 2025.The legal doctrine comprises different opinions regarding the legal nature of the EAEU and the EAEU law, which is important for the analysis of international legal aspects of the cooperation of the EAEU members in the energy sector, as well as for the formation of the EAEU energy law.The article analyzes the energy provisions of the Treaty on the Eurasian Economic Union, the role of national legislation and bilateral international treaties of the EAEU members in the energy sector.Materials and methods. The EAEU legal acts, previous acts of the EurAsEC, the existing bilateral international treaties of the EAEU members in the energy sector, the works of prominent authors affecting Eurasian integration have been used. The research methodology was based on the following theoretical methods of cognition: analysis, synthesis, induction, deduction, analogy, as well as special methods of cognition of legal phenomena and processes: comparative legal and formal legal.Results. The study summarizes the legal acts of the EAEU in the field of energy of various legal nature, highlights the gaps and weaknesses of the energy provisions of the Treaty on the Eurasian Economic Union, identifies differences in the approaches of the EAEU and EurAsEC, analyzes the provisions of the Treaty regarding national legislation and bilateral international treaties of the EAEU members in the field of energy.Discussion and conclusions. The acts of the EAEU law in the field of energy are considered as the EAEU energy law which, as well as the European Union energy law, is a part of the international energy law.The identified gaps and weaknesses in the energy provisions of the Treaty on the Eurasian Economic Union can be used to improve the EAEU energy law.Despite the development of multilateral regulation of energy cooperation through the EAEU legal acts aimed at the formation of common EAEU energy markets the national legislation of the EAEU members remains important in the field of energy and a lot of work will be required to harmonize and unify it. In addition the bilateral international treaties of the EAEU members governing energy cooperation are of great importance.
The philosophical and methodological issues of Mongolia’s civilization in the multi-polar world
Authors: Gelegpil Chuluunbaatar , Oidov Khatanbold , Zhelezniakov A. S. 
Published: April 2020

Introduction. The article is devoted to the evaluation of topical ideas of Inner Asia civilization in the multi-polar world. Without doubt, in contemporary global world it is essential for each country to creatively explore ideas and scientific civilizational theories to define its place in modern global community. Today the Mongols actively explore these theories and doctrines which have linkage to Western origin, but the research deserves a creative approach and does not fit properly the national reality and specifics.Material and methods. To define the specific features of Mongolian civilization the authors study philosophical doctrines of existence in harmony, doctrine of duality, time, Buddism – all the ideas that influenced the Mongolian views on “nation” and “democracy”, historical lessons and geopolitics.Results. At present Mongolia has a tough choice in pursuing foreign policy. Three main world development centers- the USA, China and Russia- have emerged and the threat of a potential conflict between them have increased. It is apparent that this process in the future will strongly influence the present and further development of each region and country in the world. Thus the fundamental issues concerning the methodological approach in development philosophy as well as in civilizational philosophy appeared alongside the changes in world situation. The issue of national identity should not be excluded from the political agenda as well as historical and development issues from policy analysis.Discussion and Conclusions. Mongolia in respect to its geographical location belongs to the Asia-Pacific region. In the civilizational aspect Mongols are nomadic nation. These two factors are fundamental conditions for the shaping of national identity. In context of civilizational affinity and national identity Mongols are more close to the Central Asian space. In this area such regional organizations as the Shanghai Cooperation Organization and APEC operate. However, Mongolia is not a member of these organizations and cannot efficiently participate in big regional and world projects and programs having the status of observer. Hence the membership in these regional organizations and the significant economic growth are strategically important for Mongolia.
Analysis of Perceived Factors Affecting Tourist Satisfaction in Mountain Tourism: A Study in Mount Papandayan, Indonesia
Authors: Vindiana Afina Putri , Novani Santi , Mayangsari Lidia , Alamanda Dini Turipanam 
Published: April 2020

Although the mountain is a popular tourist destination, research regarding mountain tourism is still limited. Over the years, researches on tourist satisfaction covered a more general context of tourism, without specifically analyzing mountain tourism. Therefore, this research aims to investigates factors influencing tourists’ satisfaction on mountain tourism, with Mount Papandayan, Garut Regency, West Java, Indonesia, as a case. Eleven variables regarding tourist satisfaction were derived from several interviews with Mount Papandayan’s management staff and 30 tourists that were hiking and camping on Mount Papandayan. A sample of 100 respondents, who were the Mount Papandayan’s tourists that had been hiking and camping, completed questionnaires. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) was used to analyze the data. The results show that there are three factors that affect tourist’s satisfaction in mountain tourism, which are ‘infrastructure and facilities’, ‘natural resources’, and ‘mountain area’s atmosphere’, while CFA validated the model fit. The results also indicated a significant relationship between the three factors. This research provides an opportunity for broader research opportunities in mountain tourism, especially in tropical countries.
Keywords : mountain tourism, satisfaction, EFA, CFA
Packing density and long-term occlusion after transcatheter vessel embolization with soft, bare-platinum detachable coils
Authors: Vogler IV James , Gemender Mark , Samoilov Dmitri 
Published: April 2020

Objective:
The objective of this study was to examine packing density and long-term recanalization rates after embolization with soft, bare-platinum ruby coils in the gastroduodenal artery (GDA).

Materials and Methods:
Retrospective case review of patients with hepatic malignancy who underwent coil occlusion of the GDA for radioembolization or hepatic arterial infusion chemotherapy between November 2013 and July 2018. Data on patient demographics, GDA diameter, length of coil pack, and distance between GDA origin and most proximal coil were collected. Packing density was calculated as the ratio between the volume of inserted coils and the volume of the vessel area that were filled with coil. The primary outcome was the rate of GDA recanalization, determined by review of hepatic arteriograms at follow-up.

Results:
Long-term occlusion free of recanalization was observed in 97.8% (88/90) of patients over a median follow-up time of 13.4 ± 11.3 months. Median vessel packing density was 55% (interquartile range 41–71) and procedural technical success was achieved in 100% (90/90) of patients. Of the 90 patients (72 men; mean age 63.8 ± 7.5 years), mean GDA diameter was 4.0 ± 0.8 mm and the proximal coil distance from GDA origin was 8.6 ± 3.0 mm. Mean coil pack length was 21.2 ± 7.6 mm. Recanalization occurred in 2.2% (2/90) of patients. No increase in recanalization rates with distal coil placement was observed.

Conclusions:
The study demonstrates high levels of technical success and low rates of recanalization (2.2%) when high packing densities (55%) are achieved using soft, bare-platinum ruby coils. The rate of recanalization at follow-up compares favorably to previously reported literature.
A biphasic tumor in posterior cranial fossa and the pineal region in young adult
Authors: Almahariq Fadi , Raguz Marina , Romic Dominik , Dlaka Domagoj , Oreskovic Darko , Sesar Patricija , Chudy Darko 
Published: April 2020

Background:
Biphasic tumors of the central nervous system are rarely described and mainly consisted out of the glial and mesenchymal component. The tumor originated out of both astrocytes and pinealocytes, best to our knowledge, has not been described. We present a case of a brain tumor consisted out of pilocytic astrocytoma (PA) and pineocytoma as components situated in the pineal region and posterior cranial fossa in young adult.

Case Description:
We present a 21-year-old patient with a history of intermittent headache, followed by nausea and vomiting, double vision, and dextropulsion. Magnetic resonance imaging revealed an extensive cystic-solid expansive formation in the posterior cranial fossa with a solid part in the area of the pineal gland. The patient underwent surgical resection. The pathohistological analysis showed two types of tumor cells; the major part of tumor showed features of PA, while minor part corresponded to pineocytoma.

Conclusion:
PA accounts for 5% of all gliomas and is most common in children and young adults. It usually occurs in the cerebellum, the optic pathway, third ventricular region, etc. Pineocytomas are rare, accounting up to 1% of all intracranial tumors. Since tumors origin is different, there must be complex molecular events or mutations that can lead to cell rearrangements and generation of two histologically different tissues in the same tumor mass. The course of treatment options is different for PA and pineocytoma; therefore, the case of brain mass consisted out of two different tissues can be helpful when deciding about the treatment of tumors in posterior cranial fossa and pineal region.
Rare dorsal thoracic arachnoid web mimics spinal cord herniation on imaging
Authors: Aljuboori Zaid , Boakye Maxwell 
Published: April 2020

Background:
Dorsal arachnoid webs (DAWs) are rare clinical entities that can mimic other conditions on magnetic resonance imaging (MRI). Here, we present a case of DAW that was misdiagnosed on MR as a ventral cord herniation.

Case Description:
A 35-year-old female presented with a 1-year history of lower extremity weakness and numbness. The MRI of the thoracic spine showed ventral cord displacement with syringomyelia. The computed tomography myelogram demonstrated ventral cord herniation. Intraoperatively, the patient had a dorsal thoracic web in the absence of cord herniation. Within 8 postoperative weeks, the patient had improved, and the follow-up MI showed a significant reduction in the syrinx size.

Conclusion:
On MR scans, DAWs may look like ventral cord herniation. However, the positive “scalpel sign” and syrinx, the absence of an arachnoid cyst on myelography, and the findings on cine MR help differentiate DAWs from ventral cord herniation.
Model Data Mining Time Series Untuk Prediksi Profitabilitas Perusahaan
Authors: Junaidi Muksan , Widyassari Adhika Pramita 
Published: April 2020

<strong><em>Profitabilitas merupakan rasio keuangan yang memperlihatkan kemampuan operasional perusahaan untuk menghasilkan laba operasi melalui modal sendiri. Untuk melihat kondisi kemampuan operasional perusahaan pada masa mendatang, bisa dilakukan dengan memprediksi nilai rasio tersebut. Teknik dalam menangani masalah prediksi ini adalah menggunakan model data mining. Penelitian ini bertujuan mengembangkan aplikasi prediksi profitabilitas keuangan perusahaan melalui model data mining neuro-fuzzy ANFIS dengan data time series. Data penelitian adalah data sekunder bersifat kuantitatif dari wibe site www.idx.co.id. Populasi data dari perusahaan emiten LQ45 di Bursa Efek Indonesia(BEI) tahun 2011-2016 berjumlah 45 perusahaan. Pemrosesan awal menghitung nilai rasio keuangan perusahaan yang akan dipakai sebagai data input pada model ANFIS. Simulasi dan evaluasi model menggunakan aplikasi GUI program Matlab. Perbandingan antara perhitungan rasio profitabilitas tahun yang di prediksi dengan nilai dari model ANFIS. Hasil akhir menunjukkan bahwa nilai model ANFIS sangat optimal, efisien, konsisten dan paling mendekati rata-rata nilai rasio tahun yang di prediksi sebesar 9.95% pada fungsi keanggotaan Segitiga. Sedang hasil prediksi tiga fungsi keanggotaan lainnya Trapesium, G-bell dan Gauss kurang optimal.</em></strong>
SELEKSI FITUR WARNA CITRA DIGITAL BIJI KOPI MENGGUNAKAN METODE PRINCIPAL COMPONENT ANALYSIS
Authors: Nugraha Danang Aditya , Wiguna Anggri Sartika 
Published: April 2020

Proses klasifikasi citra digital dilakukan terhadap data fitur yang menggambarkan ciri suatu citra. Dalam penelitian sebelumnya mengenai klasifikasi biji kopi untuk menentukan tingkat roasting, digunakan fitur warna RGB dan metode klasifikasi Jaringan Syaraf Tiruan dengan algoritma Back Propagation. Namun dalam penelitian tersebut didapatkan hasil klasifikasi yang kurang memuaskan akibat dari fitur yang digunakan kurang dapat memisahkan ciri dari setiap tingkat roasting biji kopi. Pada penelitian ini dilakukan penambahan fitur warna yang digunakan melalui penggunaan representasi warna RGB dan HSI, yang kemudian dihitung nilai mean, median, standar deviasi, skewness, kurtosis, dan energy pada setiap komponen warna. Dengan demikian terdapat 33 data fitur citra biji kopi. Dengan jumlah fitur yang semakin banyak, menimbulkan masalah baru dimana tidak setiap fitur mempunyai kontribusi yang signifikan dalam proses klasifikasi, sehingga diperlukan proses seleksi fitur untuk mendapatkan fitur dengan kontribusi maksimal. Pada penelitian ini metode Principal Component Analysis diujikan sebagai metode untuk seleksi fitur. Uji coba dilakukan terhadap 240 data citra digital biji kopi, dimana terdapat 120 citra biji kopi Arabika dan 120 citra biji kopi Robusta. Setiap jenis citra biji kopi tersebut terbagi menjadi tingkat roasting mentah, light roasting, medium roasting, dan dark roasting. Hasil penelitian ini didapatkan bahwa rata-rata proses training pada data fitur setelah dilakukan proses seleksi fitur mengalami peningkatan dibandingkan tanpa seleksi fitur. Hal ini terlihat dari 5 kali proses training dengan seleksi fitur didapatkan nilai akurasi terbaik hingga 90,8%, sedangkan tanpa seleksi fitur didapatkan akurasi terbaik adalah 89,6%.
Enhancement of solubility and bioavailability of modafinil using nanoparticles
Authors: Balachandar K  , Subramanian S  
Published: April 2020

The aim of the present work is to develop polymeric nanoparticles using Modafinil to improve its solubility, thereby increasing its bioavailability. Ionic-Gelation method has been employed for the preparation of nanoparticles using chitosan, a natural polymer, and TPP, a cross-linking agent. In this method, the drug is mixed with a polymer and maintained at a pH between 4.5 - 5.5, and the solution of TPP is added to the polymer-drug mixture under magnetic stirring. The size of nanoparticles prepared by this method usually ranges from 1-1000 nm. The reduction in the size of particles directly enhances the solubility of drug that increases its bioavailability. Nine formulations (F1-F9) of nanoparticles were optimized by varying the concentrations of chitosan and TPP. Prepared nanoparticles were evaluated for size, zeta potential, PDI, and drug content. Formulation F9 with 0.25% of chitosan & 0.5% of TPP concentration has been chosen as best formulation and lyophilized to obtain solid nanoparticles. The obtained nanoparticles loaded with modafinil are used for the preparation of tablets, and the in-vitro dissolution characteristics are compared with the marketed tablet of modafinil. in-vitrocharacteristics of tablets with modafinil loaded polymeric nanoparticles showed improved solubility than a marketed tablet.