bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vaginal repaired cesarean section diverticulum is beneficial in women with two prior cesarean sections
Authors: Wang Yizhi , Li Jiarui , Wang Xipeng 
Published: December 2019

Abstract
Study Objective: To evaluate the effect of vaginal repair in patients with cesarean section diverticulum (CSD) who had one or two previous cesarean sections (CSs).
Design: Observational cohort study.
Setting: Shanghai First Maternity and Infant Hospital.
Patients: A total of 248 patients with CSD who underwent vaginal repair between January 2012 and December 2014.
Interventions: Vaginal excision and suture of CSD.
Measurements: From January 2012 to December 2014, 248 women with CSD underwent vaginal repair surgery in Shanghai First Maternity and Infant Hospital. These included 193 women with one previous cesarean section and 55 women with two previous cesarean sections. Excision and suture of CSD was performed through a vaginal approach. The duration of menstruation, the length, width and depth of the CSD and thickness of the remaining muscular layer (TRM) C were evaluated before and after surgery by transvaginal three-dimensional (3D) color Doppler ultrasound.
Results: A total of 221 (89.11%) women were followed-up for more than 3 months, and 168 (67.74%) women were followed-up for more than 6 months. There were significant differences in the average duration of menstruation (7.77±2.05 and 8.02±2.06 days VS 13.99±3.71 days), the average size of CSD (5.54*9.19*5.60 and 5.75*9.04*6.18 mm VS 7.99*12.43*6.62 mm) and the TRM (7.61±2.52 and 7.60±3.00 mm VS 2.51±1.02 mm) after surgery compared with those figures before surgery. The results of this study reveal that vaginal repair could shorten the duration of menstruation and improve anatomical defects (P< 0.05). Moreover, there was no significant difference in the effect of clinical repair between women with one or two previous cesarean sections (P> 0.05).
Conclusion: In CSD patients, the clinical effectiveness of vaginal repair was equivalent between women with one or two previous cesarean sections.
Trial registration: This study was approved by the Ethics Committee of Shanghai First Maternity and Infant Hospital, affiliated with Tongji University (KS1512), and was conducted in accordance with the Declaration of Helsinki. All patients signed written informed consent to participate in this study. The author(s) agreed to provide copies of the appropriate documentation if requested.
Management of work-related common mental disorders in general practice: a cross-sectional study
Authors: Rivière Mathieu , Toullic Youenn , Lerouge Patrick , Blanchon Thierry , Leroyer Ariane , Plancke Laurent , Prazuck Thierry , Melchior Maria , Younès Nadia 
Published: December 2019

Abstract
Background: General practitioners (GPs) often manage individuals with work-related common mental disorders (CMD: depressive disorders, anxiety and alcohol abuse). However, little is known about the management characteristics. The aim of this study is to analyze GPs’ management and patterns of referral to other health professionals of patient with work-related CMD and associated factors.
Method: We used data from a cross-sectional study of 2 027 working patients of 121 GPs in the Nord – Pas-de-Calais region in France (April – August 2014). Statistical analyses focused on patients with work-related CMD detected by the GP. Descriptive analyses were used to characterize GPs’ management of the patients’ symptoms. Associations between patient, work, GP and contextual characteristics and GPs’ management were explored using modified Poisson regression models with robust variance.
Results: Among the 533 patients with work-related CMD in the study, the GPs provided psychosocial support to 88.0%, 82.4% were prescribed psychotropic treatment and 50.7% were on sick leave. Referral rates to mental health specialists and occupational physicians were respectively 39.8% and 26.1%. Several factors including patients’ characteristics (occupational and sociodemographic), GPs’ characteristics and environmental data were associated with the different type of management used by the GP.
Conclusion: Our study emphasizes the major and often lonesome role of the GP in the management of patients with work-related CMDs. Better knowledge of the way GPs manage those patients could help GPs in their practice, improve patients care and be a starting point to implement a more collaborative care approach.
Functional and population genetic features of copy number variations in two dairy cattle populations
Authors: Lee Young-Lim , Bosse Mirte , Mullaart Erik , Groenen Martien A. M. , Veerkamp Roel F. , Bouwman Aniek C. 
Published: December 2019

Abstract
Background Copy Number Variations (CNVs) are gain or loss of DNA segments that are known to play a role in shaping a wide range of phenotypes. In this study, we used two dairy cattle populations, Holstein Friesian and Jersey, to discover CNVs using the Illumina BovineHD Genotyping BeadChip aligned to the ARS-UCD1.2 assembly. The discovered CNVs were investigated for their functional impact and their population genetics features.

Results We discovered 14,272 autosomal CNVs, which were aggregated into 1,755 CNV regions (CNVR) from 451 animals. These CNVRs together cover 2.8% of the bovine autosomes. The assessment of the functional impact of CNVRs showed that rare CNVRs (MAF < 0.01) are more likely to overlap with genes, than common CNVRs (MAF ≥ 0.05). The Population differentiation index (Fst) based on CNVRs revealed multiple highly diverged CNVRs between the two breeds. Some of these CNVRs overlapped with candidate genes such as MGAM and ADAMTS17 genes, which are related to starch digestion and body size, respectively. Lastly, linkage disequilibrium (LD) between CNVRs and BovineHD BeadChip SNPs was generally low, close to 0, although common deletions (MAF 0.05) showed slightly higher LD (r2=~0.1 at 10kb distance) than the rest. Nevertheless, this LD is still lower than SNP-SNP LD (r2=~0.5 at 10kb distance).

Conclusions Our analyses showed that CNVRs detected using BovineHD BeadChip arrays are likely to be functional. This finding indicates that CNVs can potentially disrupt the function of genes and thus might alter phenotypes. Also, the population differentiation index revealed two candidate genes, MGAM and ADAMTS17, which hint at adaptive evolution between the two populations. Lastly, low CNVR-SNP LD implies that genetic variation from CNVs might not be fully captured in routine animal genetic evaluation, which relies solely on SNP markers.

Keywords: Copy number variations, Bos taurus, Linkage disequilibrium, population genetics
Systematic analysis of the basic/helix-loop-helix (bHLH) transcription factor family in pummelo (Citrus grandis) and identification of the key members involved in the response to iron deficiency
Authors: Zhang Xiao-Yong , Qiu Jie-Ya , Hui Qiu-Ling , Xu Yuan-Yuan , He Yi-Zhong , Peng Liang-Zhi , fu xing-zheng 
Published: December 2019

Abstract
Background

Iron (Fe) deficiency is a common problem in citrus production. As the second largest superfamily of transcription factors (TFs), the basic/helix-loop-helix (bHLH) proteins have been shown to participate in the regulation of Fe homeostasis and a series of other biological and developmental processes in plants. However, this family of members in citrus and their functions in citrus Fe deficiency are still largely unknown.

Results

In this study, we identified a total of 128 CgbHLHs from pummelo ( Citrus grandis ) genome that were classified into 18 subfamilies by phylogenetic comparison with Arabidopsis thaliana bHLH proteins. All of these CgbHLHs were randomly distributed on nine known (125 genes) and one unknown (3 genes) chromosomes, and 12 and 47 of them were identified to be tandem and segmental duplicated genes, respectively. Sequence analysis showed detailed characteristics of their intron-exon structures, bHLH domain and conserved motifs. Gene ontology (GO) analysis suggested that most of CgbHLHs were annotated to the nucleus, DNA-binding transcription factor activity, response to abiotic stimulus, reproduction, post-embryonic development, flower development and photosynthesis. In addition, 27 CgbHLH proteins were predicted to have direct or indirect protein-protein interactions. Based on GO annotation, RNA sequencing data in public database and qRT-PCR results, several of CgbHLHs were identified as the key candidates that respond to iron deficiency.

Conclusions

In total, 128 CgbHLH proteins were identified from pummelo, and their detailed sequence and structure characteristics and putative functions were analyzed. This study provides comprehensive information for further functional elucidation of CgbHLH genes in citrus.
Validation of RESP and PRESERVE score for ARDS patients with pumpless Extracorporeal Lung Assist (pECLA)
Authors: Petran Jan , Muelly Thorsten , Dembinski Rolf , Steuer Niklas , Arens Jutta , Marx Gernot , Kopp Ruedger 
Published: December 2019

Abstract
Background: RESP score and PRESERVE score have been validated for veno-venous Extracorporeal Membrane Oxygenation in severe ARDS to assume individual mortality risk. ARDS patients with low-flow Extracorporeal Carbon Dioxide Removal, especially pumpless Extracorporeal Lung Assist, have also a high mortality rate, but there are no validated specific or general outcome scores. Methods: In a retrospective single center cohort study we calculated and evaluated RESP, PRESERVE, and SOFA score for 73 ARDS patients with pumpless Extracorporeal Lung Assist. Additionally, demographic data and hospital mortality as well as ventilator settings, hemodynamic parameters, and blood gas measurement before and during extracorporeal therapy were recorded. Results: Pumpless Extracorporeal Lung Assist of mechanical ventilated ARDS patients resulted in an optimized lung protective ventilation, significant reduction of PaCO2, and compensation of acidosis. Scoring showed a mean score of alive versus deceased patients of 3 ± 1 versus -1 ± 1 for RESP (p < 0.01), 3 ± 0 versus 6 ± 0 for PRESERVE (p < 0.05) and 8 ± 1 versus 10 ± 1 for SOFA (p < 0.05). Using receiver operating characteristic curves, area under the curve (AUC) was 0.78 (95 % confidence interval (CI) 0.67 – 0.89, p < 0.01) for RESP score, 0.80 (95 % CI 0.70 – 0.90, p < 0.0001) for PRESERVE score and 0.66 (95 % CI 0.53 – 0.79, p < 0.05) for SOFA score. Conclusions: RESP and PRESERVE scores were superior to SOFA, as non-specific critical care score. Although scores were developed for veno-venous ECMO, we could validate RESP and PRESERVE score for pumpless Extracorporeal Lung Assist. In conclusion, RESP and PRESERVE score are suitable to estimate mortality risk of ARDS patients with an arterio-venous pumpless Extracorporeal Carbon Dioxide Removal.
The Influence of Summertime Heat Waves on Emotional Health
Authors: Huang Huanchun , Deng Xin , Yang Hailin , Chen Tunong , bai lin , Liu Junjie 
Published: December 2019

Abstract
BackgroundWith increases in global urbanization and global warming, there are corresponding increases in urban heat island effects. Heatwave disasters are occurring more frequently, bringing direct and indirect mental health hazards to urban residents. Understanding the mechanism of the impact of high ambient temperatures on emotional health is a scientific problem that needs clarification.MethodsData collected through an emotions questionnaire and temperature data measured on heatwave days were analyzed using GIS, SPSS and MATLAB software to study the influence of heatwaves on the negative emotions of middle-aged and elderly people (over 40 years old).ResultsThe results indicate that the degree of influence of high temperatures on various negative emotions differs significantly, as some emotional responses tend to fluctuate while others steadily worsen. The progression of emotional responses is distressed > irritable > nervous > hostile. With increasing temperatures, phase-based changes in emotional states occur, with different emotional states corresponding to different temperature thresholds; the temperature thresholds for distress/nervousness/hostility were shown to be 38°C/43°C, 40°C/44°C, 43°C, respectively.ConclusionAmbient temperatures exceeding 35°C have a significant negative impact on the emotional health of middle-aged and elderly people. Irritability and nervousness monotonically increase, while distress and hostility tend to fluctuate. This study contributes to the prevention and management of the harmful effects of heatwaves on emotions, providing basic information applicable to designing layouts for urban green spaces to reduce the effects of urban heat islands.
Assessment of contaminants in California drinking water: analyses by region and water system size
Authors: Bangia Komal , August Laura , Slocombe Andrew , Faust John 
Published: December 2019

Abstract
BACKGROUND While research has shown that small community water systems in the San Joaquin Valley, an agricultural region in California, experience a disproportionate amount of drinking water contamination, little is known about the extent of contamination in other California regions. Additionally, state-wide research on drinking water contamination in areas not served by community water systems (mostly private domestic well users) and research comparing contamination across system size are also limited.METHODS Using a novel method to assign drinking water and groundwater contaminant data to community water system service areas and areas outside of service area boundaries, we conducted a spatial analysis to estimate concentrations of thirteen contaminants and two drinking water standard violations by system size and California region. We developed a cumulative ranking method to evaluate which regions or system size category are most burdened by multiple drinking water pollutants. A trend test was also used to evaluate the influence of system size on contaminant concentrations.RESULTS The San Joaquin Valley, areas not served by community water systems, and small water systems had the highest cumulative rank for multiple high contaminant concentrations, most notably arsenic and nitrate. Large systems and the South Coast region, which includes Los Angeles, had the highest levels of disinfection byproducts and industrial contaminants like tetrachloroethylene. Violations, arsenic, lead, and cadmium had negative trends as system size increased (p<0.05). Industrial contaminants and disinfection byproducts had positive trends as system size increased (p<0.05).CONCLUSIONS Few large-scale studies have examined how geographic region or system size impact drinking water quality. Although not indicative of violating drinking water standards, our results show where efforts for specific contaminants can be targeted in specific regions. The results presented here can help understand where contaminant levels might be elevated, both from an individual contaminant perspective as well as where multiple elevated contaminant levels accumulate.
Age- and gender-specific trends in respiratory outpatient visits and diagnoses at a tertiary pediatric hospital in China: a 10-year retrospective study
Authors: Shi Peng , Zhang Xiaobo , Liu Lijuan , Tang Liangfeng , Li Jing , Wang Libo , Li Albert M. , Cao Yang , Xu Hong , Huang Guoying 
Published: December 2019

Abstract
Background: Respiratory infections are one of three leading causes of childhood mortality, and worldwide increase and recent plateau in childhood asthma has been reported. However, data on trends of respiratory diseases over long period of time is limited. This study aimed to determine the trends of respiratory disease outpatient visits (ROVs) and diagnoses (RODs) in one of the largest children’s teaching hospitals in China between 2009 and 2018. Methods: A retrospective study based on routine administrative data was designed and implemented according to the RECORD statement. Demographic details and diagnoses of the outpatients <18 years visiting the respiratory department of the hospital were extracted from the Hospital Information System. Age- and gender-specific trends were illustrated by calculating average annual growth rate (AGR) for ROVs and comparing change of proportion for different RODs over time. Results: There were 698054 ROVs from 285574 children (40.4% female). Average AGR of ROVs was 15.2%. Children aged 4 to < 7 years had a faster increase than other age groups. Bronchitis (27.6%), pneumonia (18.5%), pneumonia affecting other systems (18.4%), asthma and status asthmaticus (10.7%), and vasomotor and allergic rhinitis (9.2%) accounted for 84.4% of all RODs. The proportion of bronchitis decreased across years, with the concomitant increasing trend in the proportion of pneumonia. Age-specific trend in diagnoses showed the significantly increased proportion of asthma with ages, especially for the children aged 4 to < 18 years. Gender-specific trend in diagnoses showed the proportion of asthma was greater for males but the average AGR was greater for females. Conclusion: The persistent upward trend in ROVs was observed among children at different ages and a gender difference was also seen. In contrast to what has been reported, burden of asthma and allergies diseases continues to increase locally. Keywords: Respiratory infections, Asthma, Pediatrics, Outpatients, Trend study
Dose relationship between maternal milk proportion and necrotizing enterocolitis development
Authors: lin Bingchun , Sun Panpan , Xiong Xiaoyun , Zhuang Yanzhu , Chen Xueyu , Yao Fang , Yang Chuanzhong 
Published: December 2019

Abstract
Background: To examine the dose effect of maternal milk (MM) feeding on neonatal necrotizing enterocolitis (NEC) development. Methods: This retrospective study included 305 infants [birth weight<1 500 g, or gestation age (GA)<32 weeks]. Curve estimation was performed to evaluate the dose relationship between MM proportion and NEC. Then infants were grouped based on curve trend and multivariable regression analysis was conducted to prove the curve estimation.Results: Rate of NEC for the sample was 7.9% and age at onset was 25.6(16.0–26.0) days. The curve showed that when MM proportion was increased from zero to 20%, rate of NEC increased from 25% to 40% at first and when MM from 20% to 100%, rate dropped from 40% to near zero. In the MM proportion from zero to 40%, rate of NEC changed greatly. The multiple regression analysis showed that compared with MM proportion >0.70, 0.4<MM proportion £0.7 was associated with a 5.482- fold higher odds of developing NEC（P=0.032）; 0<MM proportion £0.4 was a 24.99-fold increase in the odds of developing NEC（P<0.001）; MM proportion =0 was a 9.348-fold increase in the odds of developing NEC（P=0.003）. Conclusion: This study estimated how rate of NEC changed with MM proportion increase, MM proportion being greater than 40% had a significant protection against NEC. We should encourage mother for breast milk and alert to NEC when the preterm is mixed feeding with MM proportion less than 40%.
An investigation of amino acid and acylcarnitine levels in neonates from the Tibet Autonomous
Authors: Zhang Chunyan , Dha Drun , Cheng Yuxuan , Ma Ya , Meng Yan , Tse Drun , Ngawang Dolma , Dekyi Pedrun , Jiang Tao , Shu Yang , Cui Jiayi , Li Jing , Tian Yaping 
Published: December 2019

Abstract
Background: The purpose of the study was to establish reference values of amino acids and acylcarnitines in newborns of the Tibet Autonomous Region for the first time and to provide an experimental basis for the diagnosis of genetic metabolic diseases.Methods: We detected concentrations of 43 kinds of amino acids, acylcarnitines and succinylacetone in the dried blood spots of 15029 newborns using liquid chromatography tandem mass spectrometry. We compared the indexes between Tibet and our lab, where most data come from an inland area and Han Chinese people. Then we compared amino acid and acylcarnitine levels of seven regions in Tibet and explored their impact factors. The distribution of amino acid and acylcarnitines were different in Tibet.Results: Reference intervals of amino acids and acyl carnitines in neonates from the Tibet Autonomous Region were defined according to the (P 0.5% ~ P 99.5%) of the values. Given the third reference range, the recall rate of statistical screening was significantly reduced to 2.16%.Conclusions: This study has contributed to the field by determining the actual values of amino acids and acylcarnitines in newborns from the Tibet Autonomous Region, which could be used as reference for a newborn metabolic screening project in this area.
CCL2 is associated with metastasis and poor prognosis of bladder cancer
Authors: gao wei , zhang qiwang , zhang lianhua , liu qiang , duan xuehui , bo juanjie , yang guoliang 
Published: December 2019

Abstract
Background: Chemokine (C-C motif) ligand 2 (CCL2) is an important immune factor, which may be important in cancer progression by promoting proliferation, invasion metastasis and the tumor microenvironment. Recent researches have demonstrated that overexpression of CCL2 is associated with unfavorable prognosis in various cancer types. In this study, we aim to determine the prognostic value of CCL2 expression in patients with bladder cancer (BC). Methods: We retrospectively enrolled 154 patients with bladder cancer at Renji Hospital, Shanghai Jiaotong University between 2005 and 2007. CCL2 expression was assessed by immunohistochemical staining and its association with clinicopathologic features and prognosis were evaluated. Kaplan-Meier method was applied to compare survival curves. Cox regression models were ﬁtted to analyze the effect of prognostic factors on Overall survival (OS). Results: CCL2 protein had high expression in 73 of 154 cases of BC (47%). CCL2 overexpression was signiﬁcantly associated with tumor grade (P<0.001), stage (P=0.005), and lymph node metastasis (P=0.025). The Kaplan–Meier survival analysis demonstrated that CCL2 expression was signiﬁcantly associated with DSS and OS (both P< 0.001). Multivariate analysis further demonstrated that CCL2 was an independent prognostic factor for patients with BC. Conclusion: CCL2 might be a new molecular marker to predict the prognosis of patients with BC. The novel risk classification based on combining CCL2 and TNM is more reliable than using either alone.