bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-Müllerian hormone predictive levels to determine the likelihood of ovarian hyper-response in infertile women with polycystic ovarian morphology
Authors: Sene Azadeh Akbari , Ashrafi Mahnaz , Alaghmand-Fard Nasim , Mohammadi Neda , Alisaraie Mona Mortezapour , Alizadeh Ahad 
Published: December 2019

Abstract
Background: The objective of this study was to investigate how the serum levels of Anti-Müllerian hormone (AMH) in normal-ovulatory infertile women with polycystic ovarian morphology (PCOM) is associated with ovarian hyper-response? Methods: This prospective cohort study was carried out on 100 infertile women with PCOM who were treated by antagonist/agonist-triggered stimulation protocol in Shahid Akbar-Abadi Hospital IVF center, Tehran, Iran. Serum AMH levels were measured before starting the ART cycle and the ovarian hyper-response was evaluated by retrieved oocyte numbers, estradiol levels on triggering day and the incidence of OHSS clinical signs and symptoms. Logistic regression and the area under the curve (AUC) were used to estimate the effects of AMH and accuracy of test. Results: ROC curve analysis showed that AMH had a significant performance to predict ovarian hyper-response in PCOM patients (AUC = 0.73). The estimated threshold value was 4.95 ng/ml, specificity was 74.58% (95%CI: [50.85, 93.22]), and sensitivity was 73.17% (95%CI: [48.78, 92.68]). The results of logistic regression showed that there was a significant interaction between AMH and BMI (P = 0.008) so BMI had a moderation effect. In other words, the AMH cut-off values to predict the ovarian hyper-response were different for different BMI. Conclusions: Considering the AMH cut-offs for different BMI categories would be valuable to adapt a tailored, effective and safe stimulation program for infertile women with PCOM.
Identification of lncRNA–miRNA–mRNA regulatory network associated with Primary Open Angle Glaucoma
Authors: Zhou Minwen , Lu Bing , Tan Wei , Fu Mingshui 
Published: December 2019

Abstract
Background Primary open angle glaucoma (POAG) is a complicated disorder characterized by a progressive permanent degeneration of retinal ganglion cell (RGCs) death. An increasing number of studies have suggested that long noncoding RNAs (lncRNAs) have the ability to regulate gene expression; however, thus far, the mechanisms and functions of lncRNAs in the development of POAG are still unclear.Methods Using the data from Gene Expression Omnibus (GEO), differentially expressed lncRNAs and differentially expressed mRNAs between POAG patients and controls were identified. Then, the lncRNA–miRNA–mRNA competing endogenous RNA (ceRNA) network was constructed, and the key lncRNAs in POAG were identified. A Gene Ontology (GO) analysis and a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to assess the enriched biological functions of mRNA in the ceRNA network.Results During this study, a POAG-related ceRNA network with 37 miRNA nodes, 248 lncRNA nodes, 178 mRNA nodes, and 1985 edges was constructed. In addition, four lncRNAs (DNAJC27-AS1, AF121898, OIP5-AS1, and SNX29P2) were established as hub RNAs in this ceRNA network. The functional assay showed that 18 GO terms and 17 pathways were enriched.Conclusion This study provides novel insights into the lncRNA-related ceRNA network in POAG, and the four lncRNAs were identified in the development of POAG.
Analysis of relationship between key genes and astaxanthin biosynthesis in Phaffia rhodozyma by transcript level and gene knock-out
Authors: Li Zhipeng , Chen Lina , Li Tianli , Du Xiping , He Ning , Jiang Zedong , Li Lijun , Ni Hui 
Published: December 2019

Abstract
Background Phaffia rhodozyma is a potential industrial source for production of natural astaxanthin. The synthetic mechanism of astaxanthin in P. rhodozyma is complex and unclear that blocked its development. Results In this study, eight genes related to dicyclic and monocyclic pathway in three different strains of P. rhodozyma were analyzed, and the relationship between the expression and astaxanthin biosynthesis was explored. Among these genes, crtYB (R=0.75, P<0.05) and asy genes (R=0.74, P<0.05) showed the most closely correlation with astaxanthin biosynthesis. In order to further study exact relationship, crtYB and asy genes were knocked out by homologous recombination. After crtYB knock-out, astaxanthin was decreased to be under detected line. It suggested crtYB played a role in dicyclic and monocyclic pathway. Meanwhile, the asy gene was in dicyclic pathway of astaxanthin biosynthesis, and its knock-out would promote the astaxanthin biosynthesis in monocyclic pathway, resulting in a 25.04% increase in astaxanthin production. Conclusion The possible rate-limiting enzymes were asy gene and crtYB illustrated by analysis of regression. Knock-out of asy and crtYB gene was great helpful to understand the synthetic pathway of astaxanthin, and significant to the industrial application of producing astaxanthin.
Antibacterial effect of silver nanorings
Authors: González-Fernández Sara , Lozano-Iturbe Víctor , García Beatriz , Andrés Luis J. , Menéndez Mª Fe , Rodríguez David , Vazquez Fernando , Martín Carla , Quiros Luis M 
Published: December 2019

Abstract
Background The emergence and expansion of antibiotic resistance makes it necessary to have alternative anti-infective agents, among which silver nanoparticles (AgNPs) display especially interesting properties. AgNPs carry out their antibacterial action through various molecular mechanisms, and the magnitude of the observed effect is dependent on multiple, not fully understood, aspects, particle shape being one of the most important. In this article, we conduct a study of the antibacterial effect of a new type of AgNP: silver nanorings (AgNRs), and compare it with other alternative types of AgNP synthesized in parallel using the same methodology.Results When they act on planktonic forms, AgNRs produce a smaller effect on the viability of different bacteria than nanoparticles with other structures although their effect on growth is more intense over a longer period. When their action on biofilms is analyzed, AgNRs show a greater concentration-dependent effect. In both cases it was observed that the effect on inhibition depends on the microbial species, but not its Gram positive or negative nature. Growth patterns in silver-resistant Salmonella strains suggest that AgNRs work through different mechanisms to other AgNPs. The antibacterial effect is also produced to some extent by the conditioning of culture media or water by contact with AgNPs but, at least over short periods of time, this is not due to the release of Ag ions.Conclusions AgNRs constitute a new type of AgNP, whose antibacterial properties depend on their shape, and is capable of acting efficiently on both planktonic bacteria and biofilms.
αβ-Dehydrocurvularin isolated from the fungus Aspergillus welwitschiae effectively inhibited the behaviour and development of the root-knot nematode Meloidogyne graminicola in rice roots
Authors: xiang Chao , Liu Ying , Liu Shi-Ming , Huang Ya-Fei , Kong Ling-An , Peng Huan , Liu Mao-Yan , Liu Jing , Peng De-Liang , Huang Wen-Kun 
Published: December 2019

Abstract
Background: The root-knot nematode Meloidogyne graminicola has become a serious threat to rice production as a result of the cultivation changes from transplanting to direct seeding. The nematicidal metabolites of Aspergillus welwitschiae have been investigated in vitro, and the disease control efficacy of the active compound has been evaluated under greenhouse and field conditions.Results: The active compound αβ-dehydrocurvularin (αβ-DC), isolated by nematicidal assay-directed fractionation, showed significant nematicidal activity against M. graminicola, with an median lethal concentration (LC50) value of 122.2 μg mL−1. αβ-DC effectively decreased the attraction of rice roots to nematodes and the infection of nematodes and also suppressed the development of nematodes under greenhouse conditions. Moreover, αβ-DC efficiently reduced the root gall index under field conditions.Conclusion: To our knowledge, this is the first report to describe the nematicidal activity of αβ-DC against M. graminicola. The results obtained under greenhouse and field conditions provide a basis for developing commercial formulations from αβ-DC to control M. graminicola in the future.
The role of DRAM1 in mitophagy contributes to preeclampsia regulation in mice
Authors: Chen Guoqing , Lin Ying , Chen Lu , Zeng Fa , Zhang Li , Huang Yan , Huang Pingping , Liao Lingling , Yu Yuanlan 
Published: December 2019

Abstract
Background: Preeclampsia (PE) is a pregnancy complication that is diagnosed by the new onset of hypertension and proteinuria. Although the pathogenesis of PE is still not fully understood, growing evidence indicates that oxidative stress and mitochondrial dysfunction may contribute to the progression of PE. Therefore, we aimed to determine the role of mitophagy in mitochondrial dysfunction and oxidative stress in PE. Moreover, we aimed to evaluate the role of DNA damage-regulated autophagy modulator 1 (DRAM1) in the development of PE. Results: In this study, we first constructed a mouse model of PE induced by Hif-1α and found a high level of oxidative stress, apoptosis and mitochondrial dysfunction in the placentas of PE mice. Additionally, the activity of mitophagy was decreased, and the level of DRAM1 was significantly decreased in the placentas of PE mice. To further explore the role of DRAM1 in mitophagy, DRAM1 was overexpressed in the placental tissues of PE mice. It was found that the overexpression of DRAM1 effectively improved the symptoms of PE mice and that blood lipid and urine protein levels were significantly reduced. Furthermore, DRAM1 overexpression also improved mitochondrial function and reduced oxidative stress in the placentas of PE mice. In addition, it improved mitochondrial fusion and fission and enhanced mitophagy.Conclusions: our results indicate a key role of DRAM1 in mitophagy in contributing to the regulation of PE. To our knowledge, this is the first study to confirm the role of DRAM1 in PE, and the study provides a new understanding of the pathophysiological mechanisms of PE.
Apatinib in treating clinical and biochemical recurrent ovarian cancer
Authors: Wang Zhongyu , Huang Yake , Long Ling , Zhou Li , Huang Yan , Gan Lei , Pu Aiming , Li Sufen , xie rongkai 
Published: December 2019

Abstract
Background: Apatinib, a small molecule inhibitor of vascular endothelial growth factor receptor 2 (VEGFR-2), exerts antiangiogenic effects. Taken orally, apatinib shows clinical activity in the treatment of recurrent or platinum-resistant ovarian cancer (OC) as monotherapy or in combination with other chemotherapeutic agents. We investigated the efficacy of apatinib in recurrent OC and preliminarily evaluated the clinical activity of apatinib in biochemical-only recurrent OC patients. Results: We retrospectively analyzed clinical material of 41 recurrent patients who had received apatinib monotherapy or apatinib plus chemotherapy between June 2016 and August 2018. Apatinib was administered at a 500 mg daily dose. Response was determined according to measurable disease or serum carbohydrate antigen (CA)-125 levels. Progression-free survival (PFS) was estimated by Kaplan-Meier method. All patients were evaluable, 19 (46.34%) had biochemical relapse and 22 (53.66%) had clinical relapse. The objective response rate (ORR) and disease control rate (DCR) in the overall population were 31.71% and 78.05%, respectively. The median PFS was 7 months (95% confidence interval 5.43-8.57). In patients with biochemical relapse only, the median PFS was 6 months, with ORR of 26.32% and DCR of 89.47%. Conclusions: Apatinib is a well-tolerated and effective agent in patients with recurrent OC and has the potential to delay clinical progression in patients with asymptomatic biochemical relapse.
Influential factors of postoperative outcomes of symptomatic lateral discoid meniscus
Authors: Yang ShunJie , Ding Zhong-Jun , Li Jian , Xue Yang , Chen Gang 
Published: December 2019

Abstract
Background: Discoid lateral meniscus (DLM) have a higher rate of tear and degeneration for its abnormal shape and structure. Arthroscopy is the main treatment for symptomatic DLM, however, postoperative clinical outcomes vary widely resulting from effects of diverse factors. Therefore, the present research aims to explore the factors influencing postoperative clinical outcomes of symptomatic DLM. Methods: Patients with discoid meniscus who underwent arthroscopic surgery at our hospital from 9/2008 to 9/2015 were selected according to the inclusion and exclusion criteria. Gender, body mass index (BMI), and 18 other factors were selected as potential predictors that might affect postoperative outcomes. Knee function was assessed by the Lysholm, Tegner, International Knee Documentation Committee (IKDC) and Ikeuchi scales. Univariate analyses (rank-sum test and chi-squared test) and multivariate analyses (ordered logistic regression and logistic regression) were used to detect the risk factors bearing on postoperative outcomes. P<0.05 was considered statistically significant.Results: A total of 502 patients were enrolled. Female gender was an influencing factor for Lysholm (P=0.002, odds ratio (OR)=2.713), Tegner (P=0.001, OR=2.526), IKDC (P=0.022, OR=1.735) and Ikeuchi scores (P=0.010, OR=2.164). BMI was negatively correlated with Lysholm (P=0.007, OR=1.119) and IKDC (P=0.029, OR=1.088) scores. Work intensity was negatively related to IKDC (P=0.038, OR=1.492) and Ikeuchi (P=0.014, OR=1.689) scores. Age of onset was inversely correlated with Tegner (P<0.001, OR=1.109) score. Symptoms duration and IKDC (P=0.002, OR=1.020) had an inverse correlation. Lack of cartilage lesions was a protective factor in terms of Tegner score (P<0.001, OR=0.261). Outerbridge grade was negatively correlated with Lysholm (P=0.016, OR=1.589) and Ikeuchi (P=0.017, OR=1.582) scores. Saucerization with repair was a risk factor for poor Ikeuchi scores (P=0.037, OR=4.328) in terms of subtotal/total meniscectomy.Conclusion: Arthroscopic treatment of symptomatic DLM is safe and effective. Female sex, cartilage lesions and saucerization with repair may be related to unfavourable postoperative outcomes. Clinical efficacy may worsen with increasing BMI, work intensity, symptomatic duration, age of onset and Outerbridge grade.
A Novel Mutation in the Kringle IV Domain of LPA gene leading to Familial Hyperlipoproteinemia
Authors: Li Youran , Zhang Xinyue , Wang Yizhong , Gong Fan , Yu Xiaofei , Zhang Ting 
Published: December 2019

Abstract
Background This study aims to investigate the clinical characterization and causative genetic defect of a four-generation Chinese Han family with hyperlipoproteinemia. Methods The combined use of next-generation sequencing and qPCR technique was performed to investigate genetic pathology of familial hyperlipoproteinemia. Results The clinical manifestations of the family members include hyperlipoproteinemia, early-onset hypertension, coronary heart disease, lipoma, cerebral infarction and even sudden death, and a novel heterozygous deletion of 3-16 exon of LPA gene was identified to be causative for the symptoms in the family. Conclusions A novel deletion in the LPA gene was identified in a Chinese family associated with hyperlipoproteinemia, which expands the spectrum of the LPA mutation and its associated phenotype. Keywords Copy number variation; Hyperlipoproteinemia; Kringle IV; Lipoprotein(a); LPA;
Identifying no-harm incidents in home healthcare: a cohort study using trigger tool methodology
Authors: Lindblad Marléne , Unbeck Maria , Nilsson Lena , Schildmeijer Kristina , Ekstedt Mirjam 
Published: December 2019

Abstract
Background Patient safety in home healthcare is largely unexplored. No-harm incidents may give valuable information about risk areas and system failures as a source for proactive patient safety work. We hypothesized that it would be feasible to retrospectively identify no-harm incidents and thus aimed to explore the cumulative incidence, preventability, types, and potential contributing causes of no-harm incidents that affected adult patients admitted to home Healthcare. Methods A structured retrospective record review using a trigger tool designed for home healthcare. A random sample of 600 home healthcare records from ten different organizations across Sweden was reviewed. Results In the study, 40,735 days were reviewed. In all, 313 no-harm incidents affected 177 (29.5%) patients; of these, 198 (63.2%) no-harm incidents, in 127 (21.2%) patients, were considered preventable. The most common no-harm incident types were “fall without harm,” “deficiencies in medication management,” and “moderate pain.” The type“deficiencies in medication management” was deemed to have a preventability rate twice as high as those of “fall without harm” and “moderate pain.” The most common potential contributing cause was “deficiencies in nursing care and treatment, i.e., delayed, erroneous, omitted or incomplete treatment or care.” Conclusion This study suggests that it is feasible to identify no-harm incidents and potential contributing causes such as omission of care using record review with a trigger tool adapted to the context. No-harm incidents and potential contributing causes are valuable sources of knowledge for improving patient safety, as they highlight system failures and indicate risks before an adverse event reach the patient. Keywords No-harm incident, Trigger tool, Retrospective record review, Home healthcare, Patient safety
Institutionalized Data Quality Assessments: A Critical Pathway to Improving the Accuracy of Integrated Disease Surveillance Data in Sierra Leone
Authors: Njuguna Charles Kuria , Vandi Mohamed , Mugagga Malimbo , Kanu Joseph , Liyosi Evans , Chimbaru Alexander , Gachari Wilson , Caulker Victor , Sesay Stephen , Githuku Jane , Yoti Zabulon , Yahaya Ali Ahmed , Talisuna Ambrose , Fall Ibrahima Socé 
Published: December 2019

Abstract
Background Public health agencies require valid, timely and complete health information for early detection of outbreaks. Towards the end of the Ebola Virus Disease (EVD) outbreak in 2015, the Ministry of Health and Sanitation (MoHS), Sierra Leone revitalized the Integrated Disease Surveillance and Response System (IDSR). Data quality assessments were conducted to monitor the accuracy of data generated through the IDSR system.Methods Starting 2016, regular data quality assessments (DQA)were conducted in randomly selected health facilities. A structured electronic checklist was used to interview district health management team (DHMT) members and health facility staff. We used malaria data to assess data accuracy as malaria was endemic in Sierra Leone. Verification factors (VF) calculated as the ratio of verified malaria cases in the health facility register to the number of malaria cases recorded in the national health information database, were used to assess data accuracy. Allowing a 5% margin of error, VF <95% were considered over reporting while a VF >105 was underreporting. Differences in the proportion of accurate reports in the first and fourth assessments were compared using Z-test for two proportions.Results Between 2016 -2018, four DQA were conducted in 444 health facilities where 1,729 IDSR reports were reviewed. Registers and IDSR technical guidelines were widely available in health facilities and health care workers were conversant with reporting requirements. Overall data accuracy improved from VF of 95.3% in 2016 to 100.2% in 2018. Compared to the baseline in 2016, the proportion of accurate IDSR reports in 2018 increased by 19.5% (CI 12.5% -26.5%). Over reporting was more common in private clinics and not for profit facilities while under-reporting was more common in lower level government health facilities. Leading reasons for data discrepancies included counting errors in 358 (80.6%) health facilities, and missing source documents in 47 (10.6%) health facilities.Conclusion This is the first attempt to institutionalize routine monitoring of IDSR data quality in Sierra Leone. Regular data quality assessments may have contributed to improved data accuracy over time. Data compilation errors accounted for most discrepancies and should be minimized to improve accuracy of IDSR data.
The effect of Iran's Health Transformation Plan on Hospital Performance Kerman Province
Authors: Goudarzi Reza , Gholamhoseini Mohammad Tasavon , Hekmat Somayeh Noori , Yousefzadeh Setareh , Amini Saeed 
Published: December 2019

Abstract
Background Iran has performed Health Transformation Plan(HTP) from 2014 to obtain its defined goals. This study assesses and compares the efficiency and productivity of university and non-university hospitals in Kerman provinces, Iran.Methods The data of 19 selected hospitals, two years before and two years after the Health Transformation Plan was collected in this cross-sectional study. These data included the variables of physician and nurse number, and active beds as inputs and bed occupancy rate and inpatient admission adjusted with the length of stay as outputs. Data Envelopment Analysis method used to measure hospital efficiency. Malmquist Productivity Index used to measure efficiency change model before and after the plan. The efficiency was estimated using Deap 2.1 program, and the effect of the plan on efficiency and productivity of hospitals was assessed using Stata 15.Results The results indicated that the average efficiency of all hospitals before the HTP was 0.843 and after the HTP was increased to 0.874. However, this was not significant (P>0.05). Productivity also had a decreasing trend.Conclusion Based on the results of the DEA method, it was found that the efficiency and productivity of university and non-university hospitals did not increase significantly after the HTP. Therefore, it is recommended that attention should be paid to the performance indicators of hospitals regarding how resources are allocated and decisions made.
Exploring the multi-dimensional influencing factors associated with sub-health status for residents: a cross-sectional study from China
Authors: Ren Jiaojiao , Yin Xiling , Li Guangyou , Li Jun , Zhang Liju , Zhang Na , Ren Juan , Liu Yahui 
Published: December 2019

Abstract
Background: The high incidence of sub-health and its impact on life and work have attracted wide attention. Sub-health status has been studied in China; however, there remains a lack of studies on multi-dimensional factors affecting sub-health status. This study aims to explore the sub-health status of residents, and its influencing factors in Zhuhai city of Guangdong Province of China. Methods: Data were originated from the baseline survey of Zhuhai WHO Healthy Cities Index System in 2015, which was a cross-sectional study for the influencing factors associated with sub-health status. Finally, 3,313 participants aged 16-65 years were recruited. The study used the Sub-health Measurement Scale (SHMS V1.0), and the multivariate logistic regression model was to examine their possible associations with sub-health status. Data were analyzed using the SPSS version 22.0. Results: Sub-health and non-sub-health groups accounted for 56.8% and 43.2% of the study population, respectively. There existed significant differences in terms of all items of SHMS V1.0 between the two groups. In the multivariate model, the place of residence was statistically significantly associated with sub-health, followed by having many close neighbors, relatives or friends, and happy feelings. Conclusion: There are significant differences in many items of SHMS V1.0 between sub-health and non-sub-health groups. The leading determinants of sub-health included place of residence; having close neighbors, relatives or friends; having happy feelings; and negative emotions. To develop an effective sub-health intervention program, these factors should be taken into consideration. To develop an effective sub-health intervention program, the influencing factors should be taken into consideration.