bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adverse effects on growth performance and bone development in nursery pigs fed diets marginally deficient in phosphorus with increasing calcium to available phosphorus ratios
Authors: Becker Spenser L , Gould Stacie A , Petry Amy L , Kellesvig Leah M , Patience John F 
Published: October 2020

Abstract
The objective of this experiment was to evaluate the growth performance and bone mineral content (BMC) of nursery pigs in response to increasing total calcium (Ca) to available phosphorus (aP) ratios in diets containing phytase (250 FTU/kg; Natuphos E, BASF, Florham Park, NJ). A total of 480 nursery pigs (body weight (BW) = 5.7 ± 0.6 kg) with 10 pigs per pen and 7 pens per treatment (6 pens fed 2.75:1 diet) were allotted to seven treatments consisting of increasing ratios of calcium to available phosphorus (Ca:aP): 1.25, 1.50, 1.75, 2.00, 2.25, 2.50, and 2.75. From day −7 to 0, pigs were fed a common diet. They were then fed the treatment diets during two experimental phases from day 1 to 14 and 15 to 28, respectively. Available P was formulated to 0.33% and 0.27% (approximately 90% of requirement) in dietary phases 1 and 2, respectively. BW, average daily gain (ADG), average daily feed intake (ADFI), and gain-to-feed ratio (G:F) were determined. BMC of the femur was measured on day 28 on one pig per pen using dual x-ray absorptiometry. Data were analyzed as a linear mixed model using PROC MIXED (SAS, 9.3). Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing the Ca:aP. Over the 28-d experimental period, increasing Ca:aP resulted in a linear decrease in ADG (353, 338, 328, 304, 317, 291, and 280 g/d; P &lt; 0.01), ADFI (539, 528, 528, 500, 533, 512, and 489 g/d; P &lt; 0.05), and G:F (0.68, 0.66, 0.64, 0.62, 0.61, 0.59, and 0.58; P &lt; 0.01). Increasing Ca:aP also resulted in decreased BW on days 14 and 28 (P &lt; 0.01). The BMC of the femur decreased with increasing Ca:aP (6.2, 6.3, 5.7, 5.9, 5.5, 5.6, and 5.3 g; P &lt; 0.05). Regression analysis explained the impact of Ca:aP as follows on ADG (ADG [g/d] = 339 − 36x; r2 = 0.81), G:F (G:F = 0.61 – 0.03x; r2 = 0.72), and BMC (BMC [g] = 6.4 – 0.27x; r2 = 0.43), where x is the Ca:aP. In conclusion, all outcomes indicated that any level of calcium above the minimum used in this experiment impaired growth performance and skeletal development. Further research using even lower levels of dietary Ca is warranted.
Management Strategies for Pediatric Burns During the COVID-19 Pandemic
Authors: Yaacobi (Shilo) Dafna , Ad-El Dean , Kalish Eyal , Yaacobi Eyal , Olshinka Asaf 
Published: October 2020

AbstractThe coronavirus disease 2019 (COVID-19) pandemic has been challenging global health, in many countries all non-urgent medical treatments were postponed in order to focus health systems, workforce and other resources on crucial treatments for COVID-19 patients. The pediatric cases are a minority of all COVID-19 patients and might present atypically. Due to an increase in pediatric burn cases we decided to establish an outpatient pre-hospital clinic as an intermediate “station”, in order to triage pediatric burn patients into those who present at our hospital, and those treated via telemedicine. We divided our tactics into environmental and patient management both in hospitalized and outpatient patients, also medical staff management including preventive care, surgery, and bedside procedures. We found that patients and their escorts waited longer before arriving to the Emergency Room, apparently trying to avoid the hospital visit. A higher proportion of patients was hospitalized during the pandemic (4.5% in 2020, compared to 2.6% and 2.0% in 2019 and 2018, respectively). However, the length of stay was similar to routine periods, the surgery rate and length of follow up until healing was similar to that of the same month in previous years. We assume that these factors reflect treatment quality, which was not affected, due to the use of telemedicine, and that our treatment standards were maintained. It is important to have a good regulation system of prevention and care, including the tactics described below.
Silencing Orco Impaired the Ability to Perceive Trail Pheromones and Affected Locomotion Behavior in Two Termite Species
Authors: Gao Yongyong , Huang Qiuying , Xu Huan 
Published: October 2020

Abstract
Sophisticated social behaviors in termite colonies are mainly regulated via chemical communication of a wide range of pheromones. Trail pheromones play important roles in foraging behavior and building tunnels and nests in termites. However, it is almost unclear how termites perceive trail pheromones. Here, we cloned and sequenced of olfactory co-receptor (Orco) genes from the two termites Reticulitermes chinensis Snyder (Isoptera: Rhinotermitidae) and Odontotermes formosanus (Shiraki) (Isoptera: Termitidae), and then examined their responses to trail pheromones after silencing Orco through RNA interference (RNAi). We found that Orco knockdown impaired their ability to perceive trail pheromones and resulted in the disability of following pheromone trails in the two termite species. Our locomotion behavior assays further showed that Orco knockdown significantly decreased the distance and velocity in the two termite species, but significantly increased the angular velocity and turn angle in the termite R. chinensis. These findings strongly demonstrated that Orco is essential for termites to perceive their trail pheromones, which provides a potential way to control termite pests by damaging olfactory system.
The Evolution of Sex is Tempered by Costly Hybridization in Boechera (Rock Cress)
Authors: Rushworth Catherine A , Mitchell-Olds Tom 
Published: October 2020

Abstract
Despite decades of research, the evolution of sex remains an enigma in evolutionary biology. Typically, research addresses the costs of sex and asexuality to characterize the circumstances favoring one reproductive mode. Surprisingly few studies address the influence of common traits that are, in many organisms, obligately correlated with asexuality, including hybridization and polyploidy. These characteristics have substantial impacts on traits under selection. In particular, the fitness consequences of hybridization (i.e., reduced fitness due to interspecific reproductive isolation) will influence the evolution of sex. This may comprise a cost of either sex or asexuality due to the link between hybridity and asexuality. We examined reproductive isolation in the formation of de novo hybrid lineages between 2 widespread species in the ecological model system Boechera. Seventeen percent of 664 crosses produced F1 fruits, and only 10% of these were viable, suggesting that postmating prezygotic and postzygotic barriers inhibit hybrid success in this system. The postmating prezygotic barrier was asymmetrical, with 110 of 115 total F1 fruits produced when Boechera stricta acted as maternal parent. This asymmetry was confirmed in wild-collected lineages, using a chloroplast phylogeny of wild-collected B. stricta, Boechera retrofracta, and hybrids. We next compared fitness of F2 hybrids and selfed parental B. stricta lines, finding that F2 fitness was reduced by substantial hybrid sterility. Multiple reproductively isolating barriers influence the formation and fitness of hybrid lineages in the wild, and the costs of hybridization likely have profound impacts on the evolution of sex in the natural environment.
Capsular closure in patients with femoroacetabular impingement syndrome (FAIS): results of a matched-cohort study from the Danish hip arthroscopy registry
Authors: Mygind-Klavsen Bjarne , Lund Bent , Grønbech Nielsen Torsten , Kraemer Otto , Hölmich Per , Maagaard Niels , Winge Søren , Lind Martin 
Published: October 2020

ABSTRACT
Capsular closure in femoroacetabular impingement syndrome (FAIS) patients during hip arthroscopy procedures is debated. The Danish Hip Arthroscopy Registry (DHAR) contains data to perform matched-cohort analyses. The purpose of this study is to evaluate 1-year subjective outcome data from DHAR after hip arthroscopy for FAIS with capsular closure and compare these outcome data with a matched-cohort study group. The primary hypothesis was that there would be no difference in Copenhagen Hip and Groin Outcome Score (HAGOS) subjective outcome between patients with and without capsular closure. This is a retrospective cohort study (level of evidence, 3). FAIS patients eligible for hip arthroscopy between January 2012 and December 2017, and where the interportal capsulotomy was closed, were identified and matched with patients without capsular closure. Matching criteria were gender (1:1), age (±5 years), degree of cartilage injury: ICRS and modified Becks grade (±1 grade) and radiological parameters: lateral center edge angle and alpha angle (±10°). A comparison between cohorts regarding differences in patient outcome scores, HAGOS, Hip Sports Activity Scale (HSAS), EuroQol-5 Domain (EQ-5D) and numeric rating scale (NRS) pain at 1-year follow-up were performed. Wilcoxon rank-sum test was used to compare differences between preoperative and postoperative subjective outcome scores, level of statistical significance was 0.05. A total of 189 patients were included in the capsular closure group and matched with 189 control patients. The mean age in years (±standard deviation) was 39.4 (±11.8) and 39.3 (±11.2), respectively, 55% females. Both groups improved significantly at 1-year follow-up. Significant improvements in the capsular closure group were found in HSAS, EQ-5D, NRS pain (rest and walk) and most HAGOS subscales compared with the non-closure group. All patients underwent labral repair in combination with both femoral osteochondroplasty and acetabuloplasty. The revision rate reported after 2 years was 6.8% in the non-closure group and 3.5% in the closure group. One patient in each cohort received a total hip replacement after 2 years. Capsular closure during arthroscopic FAIS treatment resulted in better subjective outcomes and less pain during rest and walking compared with matched controls. Both groups demonstrated improved outcome at 1-year follow-up. Furthermore, capsular closure might result in a lower risk of a revision hip arthroscopy.
New Population of the Rare Dragonfly Ophiogomphus howei (Odonata: Gomphidae) in Southern Michigan, United States
Authors: Craves Julie A , O’Brien Darrin S , Marvin David A 
Published: October 2020

AbstractOphiogomphus howei Bromley is a rare North American dragonfly, given a global conservation rank of Vulnerable by NatureServe. This species inhabits localized stretches of a limited number of typically undisturbed, high-quality, forested rivers in two disjunct regions in North America. We describe a new population in between the known ranges from an impaired river in a largely urban watershed in southern Michigan, United States. We also report a previously overlooked specimen from a new location in Pennsylvania, United States, and provide current occurrence and conservation status of the species in North America.
Species-specific environmental conditions for winter bat acoustic activity in North Carolina, United States
Authors: Parker Kevin A , Li Han , Kalcounis-Rueppell Matina C 
Published: October 2020

AbstractLow winter temperatures are a major driver of hibernation and migration in temperate North American bats. Hibernation and migration in turn affect bat mortality via white-nose syndrome and collisions with wind turbines. To describe winter bat acoustic activity across a wide temperature gradient and to understand species-specific responses to low temperatures, we recorded nightly acoustic activity of bats at 15 sites across the state of North Carolina, United States, from December through February 2016 – 2018. Bat acoustic activity was recorded at all sites during both winters. Nightly probability of bat acoustic presence regressed positively on ambient temperature. Nightly probability of presence in Lasionycteris noctivagans (silver-haired bat) and Eptesicus fuscus (big brown bat) regressed negatively on wind speed. The mean probability of presence within the same winter condition was highest for L. noctivagans, followed by E. fuscus, Perimyotis subflavus (tricolored bat), and Lasiurus cinereus (hoary bat). Differences in species’ mean body weight and roosting preference explained part of the variation of the species-specific probability of presence. Our results can be used to predict bat acoustic presence for these species across the southeastern United States in winter, and better understand the potential threats to bats such as white-nose syndrome and wind turbine interactions.
Infiltrating Hematogenous Macrophages Aggregate Around β-Amyloid Plaques in an Age- and Sex-Dependent Manner in a Mouse Model of Alzheimer Disease
Authors: Kozyrev Natalie , Albers Shawn , Yang Jennifer , Prado Vania F , Prado Marco A M , Fonseca Gregory J , Rylett R Jane , Dekaban Gregory A 
Published: October 2020

Abstract
β-Amyloid (Aβ) plaques can trigger chronic inflammation in the cellular environment that recruits infiltrating macrophages during the course of Alzheimer disease (AD). Activated macrophages release pro-inflammatory cytokines that increase neurotoxicity associated with AD. A major impediment to investigating neuroinflammation involving macrophage activity is the inability to discriminate resident microglial macrophages (mMϕ) from hematogenous macrophages (hMϕ), as they are morphologically and phenotypically similar when activated. To distinguish between mMϕ and hMϕ and to determine their respective roles in chronic inflammation associated with the progression of amyloidosis, we used lys-EGFP-ki transgenic mice that express enhanced green fluorescent protein in hMϕ, but not in mMϕ. These mice were crossed with 5XFAD mice. The offspring demonstrated robust AD pathology and enabled visual discrimination of mMϕ from hMϕ. Mutant mice demonstrated robust increases in Aβ1–42, area of Aβ plaques, gliosis and deficits in spatial learning by age 5 months. The time-course of Aβ accumulation, paralleled by the accumulation of hMϕ around Aβ plaques, was more robust in female compared with male mice and preceded behavioral changes. Thus, the accumulation of infiltrating hMϕ around Aβ plaques was age- and sex-dependent and preceded cognitive impairment.
Recovering Refugee Stories: Chilean Refugees and World University Service
Authors: Ribeiro de Menezes Alison 
Published: October 2020

Abstract
Within the context of a special section of the Journal of Refugee Studies, this article charts and evaluates the work of the UK-based NGO, World University Service (WUS), in assisting Chileans who fled their country in the wake of the 1973 coup and subsequent Pinochet dictatorship. The article combines documentary research in the WUS archive, Modern Records Centre, University of Warwick, and a series of in-depth interviews with 26 Chileans who were assisted by WUS. It begins by outlining the history, structure and development-based focus of the WUS scholarship scheme, which was an important step in the establishment of formal refugee support structures in the UK. It also explores quantitative data of the scheme’s academic success, before enriching this with qualitative research focused on analysis of refugees’ individual life stories. The research findings reveal tensions between what Chanfrault-Duchet calls the ‘inner self’ and the ‘social self’ in the case of women interviewees. The article concludes that a focus on individual life stories as emplotted narratives can enrich quantitative understandings of the effectiveness of formal refugee support programmes and provide important insights into the grassroots experience of exile, helping to avoid the historical decontextualization of discussions of refugee support programmes.
FLOWERING LOCUS T4delays flowering and decreases floret fertility in barley
Authors: Pieper Rebecca , Tomé Filipa , Pankin Artem , von Korff Maria 
Published: October 2020

AbstractFLOWERING LOCUS T-like (FT-like) genes control the photoperiodic regulation of flowering in many angiosperm plants. The family of FT-like genes is characterized by extensive gene duplication and subsequent diversification of FT functions which occurred independently in modern angiosperm lineages. In barley, there are 12 known FT-like genes (HvFT), but the function of most of them remains uncharacterized. This study aimed to characterize the role of HvFT4 in flowering time control and development in barley. The overexpression of HvFT4 in the spring cultivar Golden Promise delayed flowering time under long-day conditions. Microscopic dissection of the shoot apical meristem revealed that overexpression of HvFT4 specifically delayed spikelet initiation and reduced the number of spikelet primordia and grains per spike. Furthermore, ectopic overexpression of HvFT4 was associated with floret abortion and with the down-regulation of the barley MADS-box genes VRN-H1, HvBM3, and HvBM8 which promote floral development. This suggests that HvFT4 functions as a repressor of reproductive development in barley. Unraveling the genetic basis of FT-like genes can contribute to the identification of novel breeding targets to modify reproductive development and thereby spike morphology and grain yield.
Differences in Postmating Transcriptional Responses between Conspecific and Heterospecific Matings inDrosophila
Authors: Ahmed-Braimah Yasir H , Wolfner Mariana F , Clark Andrew G 
Published: October 2020

AbstractIn many animal species, females undergo physiological and behavioral changes after mating. Some of these changes are driven by male-derived seminal fluid proteins and are critical for fertilization success. Unfortunately, our understanding of the molecular interplay between female and male reproductive proteins remains inadequate. Here, we analyze the postmating response in a Drosophila species that has evolved strong gametic incompatibility with its sister species; Drosophila novamexicana females produce only ∼1% fertilized eggs in crosses with Drosophila americana males, compared to ∼98% produced in within-species crosses. This incompatibility is likely caused by mismatched male and female reproductive molecules. In this study, we use short-read RNA sequencing to examine the evolutionary dynamics of female reproductive genes and the postmating transcriptome response in crosses within and between species. First, we found that most female reproductive tract genes are slow-evolving compared to the genome average. Second, postmating responses in con- and heterospecific matings are largely congruent, but heterospecific matings induce expression of additional stress-response genes. Some of those are immunity genes that are activated by the Imd pathway. We also identify several genes in the JAK/STAT signaling pathway that are induced in heterospecific, but not conspecific mating. While this immune response was most pronounced in the female reproductive tract, we also detect it in the female head and ovaries. These results show that the female’s postmating transcriptome-level response is determined in part by the genotype of the male, and that divergence in male reproductive genes and/or traits can have immunogenic effects on females.
The IMG/M data management and analysis system v.6.0: new tools and advanced capabilities
Authors: Chen I-Min A , Chu Ken , Palaniappan Krishnaveni , Ratner Anna , Huang Jinghua , Huntemann Marcel , Hajek Patrick , Ritter Stephan , Varghese Neha , Seshadri Rekha , Roux Simon , Woyke Tanja , Eloe-Fadrosh Emiley A , Ivanova Natalia N , Kyrpides Nikos C 
Published: October 2020

AbstractThe Integrated Microbial Genomes & Microbiomes system (IMG/M: https://img.jgi.doe.gov/m/) contains annotated isolate genome and metagenome datasets sequenced at the DOE’s Joint Genome Institute (JGI), submitted by external users, or imported from public sources such as NCBI. IMG v 6.0 includes advanced search functions and a new tool for statistical analysis of mixed sets of genomes and metagenome bins. The new IMG web user interface also has a new Help page with additional documentation and webinar tutorials to help users better understand how to use various IMG functions and tools for their research. New datasets have been processed with the prokaryotic annotation pipeline v.5, which includes extended protein family assignments.
Assessment of Kidney Injury as a Severity Criteria for Clostridioides Difficile Infection
Authors: Carlson Travis J , Gonzales-Luna Anne J , Nebo Kimberly , Chan Hannah Y , Tran Ngoc-Linh T , Antony Sheena , Lancaster Chris , Alam M Jahangir , Begum Khurshida , Garey Kevin W 
Published: October 2020

Abstract

Background
The Infectious Diseases Society of America (IDSA) and Society for Healthcare Epidemiology of America (SHEA) revised their Clostridioides difficile infection (CDI) severity classification criteria in 2017 to include an absolute serum creatinine (SCr) value above a threshold (≥1.5 mg/dL) rather than a relative increase from baseline (≥1.5 times the premorbid level). To date, how to best define kidney injury as a CDI disease severity marker has not been validated to assess severe outcomes associated with CDI.

Methods
This multicenter cohort study included adult hospitalized patients with CDI. Patients were assessed for the presence of acute kidney injury (AKI), chronic kidney disease (CKD), and CDI severity using the 2010 and 2017 IDSA/SHEA CDI guidelines. Primary outcome was all-cause inpatient mortality.

Results
The final study cohort consisted of 770 CDI episodes from 705 unique patients aged 65 ± 17 years (female, 54%; CKD, 36.5%; AKI, 29.6%). Eighty-two episodes (10.6%) showed discordant severity classification results due to the inclusion of more patients with preexisting CKD in the severe disease category using an absolute SCr threshold criterion. The absolute SCr criterion better correlated with all-cause mortality (odds ratio [OR], 4.04; 95% confidence interval [CI], 1.76–9.28; P = .001) than the relative increase in SCr (OR, 1.34; 95% CI, 0.62–2.89; P = .46). This corresponded to an increased likelihood of the 2017 CDI severity classification criteria to predict mortality (OR, 5.33; 95% CI, 1.81–15.72; P = .002) compared with the 2010 criteria (OR, 2.71; 95% CI, 1.16–6.32; P = .02).

Conclusions
Our findings support the 2017 IDSA/SHEA CDI severity classification criteria of a single pretreatment SCr in future CDI guideline updates.
Hemifacial Spasm: Endoscopic Assistance in Facial Nerve Decompression With Lateral Spread Response Corroboration: 2-Dimensional Operative Video
Authors: Brimley Cameron J , Sampath Raghuram 
Published: October 2020

Abstract
This video depicts the case of a 48-yr-old female with 3 yr of progressive left hemifacial spasm (HFS) refractory to medication. Magnetic resonance imaging showed a large anterior inferior cerebellar artery (AICA) and also a labyrinthine artery loop around the facial nerve (FN) root exit zone. A large bony eminence was also noted in the superior and lateral aspects of the porous acousticus (PA). She preferred surgery if “cure” was possible in lieu of Botox injections. A left retro sigmoid craniotomy was performed with brainstem auditory evoked responses (BAERs) and FN monitoring along with lateral spread response (LSR) assessment. The large bony prominence was drilled in its lateral aspect. Despite this, visualization was still limited and therefore we utilized a 30-degree-angled endoscope to observe the vessels caudal and cranial to the FN. This view prompted us to then drill further at the PA to decompress the FN as well as mobilize the labyrinthine artery away from the nerve. The LSR showed a dramatic improvement when FN decompression was accomplished, and then a further improvement with arterial mobilization and Teflon pledget placement. The BAERS remained at baseline throughout. FN function and hearing were intact on postoperative clinical assessment. Her symptomatic improvement was recorded at 12 mo after surgery.
This video illustrates a more complex case of microvascular decompression with skull base concepts and techniques. The patient provided consent for the procedure and use of her images and operative video for publication.