bluuebunny/crossref_metadata_2025_split huggingface dataset:
The disparity of maternal childcare pressure and quality of life between children diagnosed with autism spectrum disorder and developmental delay in the Chinese culture context: Taiwan Birth Cohort pilot Study
Authors: Lung For-Wey , Shu Bih-Ching , Chiang Tung-Liang , Lin Shio-Jean 
Published: April 2020

Abstract
Background: Many studies have shown that parents express higher stress when taking care of children with autism spectrum disorder (ASD). However, cultural context should be taken into consideration when investigating children's diagnosis and parental quality of life (QOL). Therefore, this study aimed to investigate the pathway relationship among parental QOL, child development, ASD and developmental delay (DD) risk at 3 years of age, and ASD or DD diagnosis 2.5 years later.Methods: A birth cohort study database collected at age 3 and 5.5 years was used (N=1618). The Modified Checklist of Autism in Toddlers, Taiwan Birth Cohort Study Developmental Instrument, and the Short Form-36 was used to assess ASD risk, developmental condition (DD risk), and parental QOL, respectively.Results: By structural equation modeling, an indirect influence was found between children's diagnosis and maternal health QOL. Mothers of children diagnosed with ASD perceived more family support, and those who perceived more family support had better health QOL. Conversely, mothers of children diagnosed with DD perceived higher childcare pressure, and mothers who perceived higher childcare pressure had worse health QOL.Conclusions: The differences in mother's experience when their children are diagnosed with DD and ASD can only be found in pathway analysis, showing the importance of structural equation models. Healthcare personnel should take into consideration the cultural context of the families we encounter, and the vital role family support plays should be taken into consideration when interacting with families from collectivism cultures.
Development of the IcanSDM scale to assess primary care clinicians’ ability to adopt shared decision-making
Authors: Giguère Anik , Bogza Laura-Mihaela , Coudert Laëtita , Carmichael Pierre-Hugues , Renaud Jean-Sébastien , Légaré France , Lindig Anja , Voyer Philippe 
Published: April 2020

Abstract
Objective: To support studies on the implementation of shared decision making (SDM), we sought to develop and validate the IcanSDM scale that assesses clinicians’ perceptions of their ability to adopt SDM. Results : An expert panel reviewed the literature on clinician-reported barriers to SDM adoption, to create an 11-item preliminary scale. A convenience sample of 16 clinicians from Québec (Canada) completed the IcanSDM and the Belief about capabilities subscale of the CPD-Reaction instrument (BCap), before and after SDM training. We audio-recorded their comments as they completed the scale. We measured IcanSDM’s internal consistency, sensitivity to change and correlation with BCap. Partial correlation coefficients and item analyses suggested removing three items. In the 11-item IcanSDM version, three items lacked clarity or responsiveness, or showed negative partial correlations with the whole instrument. We thus removed these items. The revised 8-item version gave Cronbach’s alphas of 0.63 before and 0.71 after training, and a 16% improvement in IcanSDM total score after training, compared to before training (p<0.0001). We also found a significant correlation between IcanSDM and the BCap before training (p=0.02), but not after (p=0.46).
Dual hit rat models of diabetic cardiomyopathy and nephropathy: pancreatectomy combined with isoprenaline treatment or uninephrectomy
Authors: Thisted Louise , Østergaard Mette V , Pedersen Annemarie A , Pedersen Philip J , Lindsay Ross T , Murray Andrew J , Fink Lisbeth N , Pedersen Tanja X , Secher Thomas , Johansen Thea T , Thrane Sebastian T , Skarsfeldt Torben , Jelsing Jacob , Thomsen Morten B , Zois Nora E 
Published: April 2020

Abstract
Background Cardiovascular and renal complications are the predominant causes of morbidity and mortality amongst patients with diabetes. Development of novel treatments have been hampered by the lack of available animal models recapitulating the human disease. We hypothesized that experimental diabetes in rats combined with a cardiac or renal stressor, would mimic diabetic cardiomyopathy and nephropathy, respectively. Methods Diabetes was surgically induced in male Sprague Dawley rats by 90% pancreatectomy (Px). Isoprenaline (Iso, 1 mg/kg, sc., 10 days) was administered five weeks after Px with the aim of inducing cardiomyopathy, and cardiac function and remodeling was assessed by echocardiography ten weeks after surgery. Left ventricular (LV) fibrosis was quantified by Picro Sirius Red and gene expression analysis. Nephropathy was induced by Px combined with uninephrectomy (Px-UNx). Kidney function was assessed by measurement of glomerular filtration rate (GFR) and urine albumin excretion, and kidney injury was evaluated by histopathology and gene expression analysis. Results Px resulted in stable hyperglycemia, hypoinsulinemia, decreased C-peptide, and increased glycated hemoglobin (HbA1c) compared with sham-operated controls. Moreover, Px increased LV mass and dimensions, and caused a shift from α-myosin heavy chain (MHC) to β-MHC gene expression. Isoprenaline treatment, but not Px, decreased ejection fraction and induced LV fibrosis. There was no apparent interaction between Px and Iso treatment. The superimposition of Px and UNx increased GFR, indicating hyperfiltration. Compared with sham-operated controls, Px-UNx induced albuminuria and increased urine markers of kidney injury, including neutrophil gelatinase-associated lipocalin (NGAL) and podocalyxin, concomitant with upregulated renal gene expression of NGAL and kidney injury molecule 1 (KIM-1). Conclusion Whereas Px and isoprenaline separately produced clinical endpoints related to diabetic cardiomyopathy, the combination of the two did not accentuate disease development. Conversely, Px in combination with UNx resulted in several clinical hallmarks of diabetic nephropathy indicative of early disease development.
Access to and use of sexual and reproductive health services among street children in Uganda: Does Migration matter?
Authors: Bwambale Mulekya Francis , Bukuluki Paul , Moyer Cheryl A. , Borne Bart H.W Van den 
Published: April 2020

Abstract
Background

While the nexus of migration and health outcomes is well acknowledged, the effect of rural-urban migration on uptake of sexual and reproductive health (SRH) services has received less attention. We assessed the effect of rural-rural urban migration on uptake of SRH services and whether there is a difference in uptake of SRH services among migrant and non-migrant street children.

Methods

Data were collected among 513 street children aged 12-24 years using face‐to‐face interviews. Participants were recruited by venue-based time-space sampling (VBTS). Using STATA 16.00, we performed multivariate binary logistic regression to identify correlates of SRH service uptake with migration status as the main predictor while controlling for confounding. In this study, migrants are defined as children who had moved to settle in the city for at least 24 months preceding the survey.

Results

Overall, two-thirds (66.82%) of children had moved to the city in the last 2 years, 20% in the last 3-5 years and 13.18% in 6 years or longer. Only 18.13% of the street children had ever used contraception/family planning, 45.89% had ever tested for HIV and knew their status while 34.70% had ever been screened for sexually transmitted infections. Multivariate analysis shows that migrant street children had reduced odds of using sexual and reproductive health services (HIV testing, use of contraception and screening for STIs) compared to the lifelong native street children (aOR=0.59, 95%CI 0.36- 0.97). Other factors associated with use of SRH services among street children include age (aOR=4.51; 95%CI 2.78-7.33), schooling status (aOR=0.34; 95%CI -1.830 0.15-0.76), knowledge of place of care (OR=3.37,95%CI 2.04-5.34) and access to SRH education information (aOR=2.7, 95%CI 1.67- 4.53).

Conclusion

Rural-urban migration is associated with low SRH service uptake. Access to and use of SRH services among migrant street children is low compared to non-migrant street children. Our findings point to the need for urban health systems to design effective interventions to improve equitable access to and use of quality SRH services among street children while taking into consideration their migration patterns.
Full chloroplast genome assembly and phylogeny of “red and “yellow” Bixa orellana, “achiote”; popular source of food coloring and traditional medicine.
Authors: Vallejo Jorge Villacres , Ventura José Aranda , Wallis Anna , Cagle Robin , Handy Sara M. , Davis Jeffery , Pava-Ripoll Monica , Erickson David , Ramachandran Padmini , Ottesen Andrea 
Published: April 2020

Abstract
BackgroundSeeds from Bixa orellana, commonly known as “achiote” and “annatto” produce bixin and norbixin apocarotenoids which impart bright red and orange colors that have been used for thousands of years for food, medicine and body painting by indigenous Americans, and by Europeans for ~ 500 years as food coloring, especially for cheeses. Use of Bixa colorants continues to grow as synthetic dyes come under increased scrutiny for toxicity to human and environmental systems. There is a wide range of color variation in pods of Bixa orellana for which genetic loci that delineate phenotypes have not yet been identified. Whole chloroplast genomes and raw genome skims provide a wide variety of genetic markers that can be used for identification purposes as well as phylogenetic inference of broad scale evolutionary relationships. Here we apply whole chloroplast genome sequencing of “red” and “yellow” individuals of Bixa orellana for phylogenetic analyses to explore the position of Bixaceae relative to other families within the Malvales as well as to underpin future work that may delineate diverse color phenotypes.ResultsFully assembled chloroplast genomes were produced for both red and yellow Bixa orellana accessions (158,918 and 158,823 bp respectively). Synteny and gene content was identical to the only other previously reported full chloroplast genome of Bixa orellana (NC_041550). We observed a 17 base pair deletion at position 58399-58415 in both of our accessions, relative to NC_041550 and a 6 base pair deletion at position 75531-75526 in the accession of “red” Bixa. A phylogeny based on alignment free kmer distance metrics was used to confirm monophylly of Bixa accessions, and to place Bixaceae relative to other families within the Malvales.ConclusionsOur data support Bixaceae as sister to Malvaceae and identified several potentially diagnostic insertion-deletion mutations that may with future work, reliably distinguish between red and yellow phenotypes. In addition to utility for phylogenic questions and development of identity markers, we demonstrate that chloroplast genomes can be used in conjunction with modern bioinformatic search tools (kmer based) to provide rapid and precise identification of Bixa orellana for Next Generation Sequencing approaches to natural product authentication.
Increased E2F2 predicts poor prognosis in patients with HCC based on TCGA data
Authors: Zeng Zhili , Cao Zebiao , Tang Ying 
Published: April 2020

Abstract
Background E2F transcription factor 2(E2F2) plays an important role in the development and progression of various tumors, but its association with hepatocellular carcinoma remains unknown.The aim of our study was to investigate the role and clinical significance of E2F2 in hepatocellular carcinoma (HCC). Methods HCC raw data were extracted from TCGA. Wilcoxon signed-rank test, Kruskal-Wallis test and logistic regression was applied to analyze the the relationship between the expression of E2F2 and clinicalpathologic charateristics. Cox regression and Kaplan-Meier was employed to evaluate the correlation between clinicopathologic features and survial. The biological function of E2F2 was annotated by Gene set enrichment analysis (GSEA). Results The expression of E2F2 was increased in HCC samples. The expression of elevated E2F2 in HCC samples was prominentlly correlated with tumor grade (OR =2.62 for G3-4 vs. G1-2, p=1.80E-05), tumor stage (OR =1.74 for III-IV vs. I-II, p=0.03), topography (OR =1.64 for T3-4 vs.T1-2, p=0.04), tumor status (OR =1.88 for with tumor vs. tumor free, p= 3.79E-03), plasma AFP value (OR =3.18 for AFP≥400 vs AFP<20, p= 2.16E-04; OR=2.50 for 20≤AFP<400 vs AFP<20, p=2.56E-03). Increased E2F2 had a unfavorable OS (p=7.468e−05), PFI (p=3.183e−05), DFI (p=0.001), DSS (p=4.172e−05). Elevated E2F2 was independently bound up with OS (p = 4.4E-04, HR = 2.4 (95% CI [1.5-3.8])) and DSS (p = 7.6E-04, HR = 3.0, (95% CI [1.6-5.6])). GSEA disclosed that cell circle, RNA degradation, pyrimidine metabolism, base excision repair, aminoacyl tRNA biosynthesis, DNA replication, p53 signalling pathway, nucleotide excision repair, ubiquitin mediated proteolysis, citrate cycle TCA cycle were notably enriched in E2F2 high expression phenotype. Conclusions Elevated E2F2 can be a promissing independent prognostic biomarker and therapeutic target for HCC. Additionally, cell cycle, pyrimidine metabolism, DNA replication, p53 signalling pathway, ubiquitin mediated proteolysis, the citrate cycle TCA cycle may be the key pathway by which E2F2 participate in the initial and progression of HCC.
Sex differences in the association of abdominal adipose tissue measurements by using quantitative computed tomography (QCT) with untreated hypertension in a Chinese population
Authors: Chen Youzhou , Zhang Zhuoli , Wang Jihong , Sun Huayi , Zhao Xingshan , zhao qiong , Cheng Xiaoguang 
Published: April 2020

Abstract
Background: There are inconsistent results regarding the relationships of adiposity anthropometric indices and blood pressure (BP) and hypertension, and whether these relationships differ by sex is unclear. We aimed to elucidate the associations of adiposity indices measured using quantitative computed tomography (QCT) with BP and hypertension and to determine the effect of sex on the relationships in a Chinese population. Methods: Abdominal adipose fat, including visceral adipose tissue (VAT) area and subcutaneous adipose tissue (SAT) area, was measured by QCT in 1488 participants. Body mass index (BMI), waist circumference (WC), hip circumference (HC) and systolic (SBP) and diastolic BP (DBP) were measured. Results: Compared to women, men had significantly greater VAT but less SAT regardless of hypertension status. VAT, SAT and WC correlated more with SBP in men than in women. After controlling for body weight, height and age, VAT area and WC in women and VAT area in men were positively associated with SBP and DBP level. VAT area correlated more with the increased risk of hypertension in men than in women [men: odds ratio (OR) = 1.013, women: OR = 1.011]. WC had a significant correlation with an increased risk of hypertension in women but a borderline association in men (P = 0.059) when adjusted for VAT area and SAT area. Conclusions: The association of abdominal adiposity with blood pressure components and hypertension differs qualitatively by sex. WC may be an important determinant of hypertension and may be used for risk stratification for hypertension among Chinese individuals.
Potential impact of climate change on the spatial distribution of key forest tree species in Italy under RCP4.5 for 2050s
Authors: Pecchi Matteo , Marchi Maurizio , Moriondo Marco , Forzieri Giovanni , Ammoniaci Marco , Bernetti Iacopo , Bindi Marco , Chirici Gherardo 
Published: April 2020

Abstract
Background: Forests provide a range of ecosystem services essential for the human wellbeing and their ability is influenced by climate background and further connected to forest management strategies. Italy is a well-known biodiversity hotspot but an uncertainty assessment of the potential impact of climate change is still missing in this country. The aim of this paper is model the potential impact of climate change on 19 tree species occurring across the Italian forests using a species distribution modelling approach, six different Global Circulation Models (GCMs) and one Regional Climate Models (RCMs) for 2050s under an intermediate forcing scenario (RCP 4.5). Results: While no sensible variation in the spatial distribution of the total forested area has been predicted with some tree species gaining space and covering the spatial contractions of others, results showed substantial differences between each species and different climate models. The analyses reported an unchanged amount of total land suitability to forest growth in mountain areas while smaller values were predicted for valleys and floodplains than high-elevation areas. Pure woods were predicted as the most influenced when compared with mixed stands which are characterized by a greater species richness and therefore a supposed higher level of biodiversity and resilience to climate change threatens. Pure softwood stands (e.g. Pinus, Abies) were more sensitive than hardwoods (e.g. Fagus, Quercus), probably due to their artificial origin which established pure stands with tree species generally more prone to admixture with others in (semi)-natural ecosystems.Conclusions: Forest management could play a fundamental role to reduce the potential impact of climate change on forest ecosystems. Silvicultural practices should be aimed at increasing the species richness and favouring hardwoods currently growing as dominating species under conifers canopy, stimulating the natural regeneration, gene flow and supporting (spatial) migration processes.
Impairment-driven cancer rehabilitation in patients with neoplastic spinal cord compression using minimally invasive spine stabilization: A single-center retrospective study
Authors: Ishida Yukako , Shigematsu Hideki , Tsukamoto Shinji , Morimoto Yasuhiko , Iwata Eiichiro , Okuda Akinori , Kawasaki Sachiko , Tanaka Masato , Fujii Hiromasa , Tanaka Yasuhito , Kido Akira 
Published: April 2020

Abstract
Background: Neoplastic spinal cord compression is a cause of severe disability in cancer patients. To prevent irreversible paraplegia, a structured strategy is required to address the various impairments present in cancer patients. Methods: We retrospectively reviewed 27 consecutive patients with neoplastic spinal compression who were treated with minimally invasive spine stabilization (MISt). We classified the impairments of patients through our multidisciplinary tumor board based on spine-specific factors, skeletal instability and tumor growth. The neurological deficits, ambulation status, progress of pathological fracture, incidence of vertebral collapse, postoperative implant failure were examined. Changes of the Barthel index (BI) scores before and after surgery were investigated throughout the clinical courses. Results: The average duration to ambulation was 7.19±11 days, and we observed no collapse or progression of paralysis except in four cases of complete motor paraplegia before the surgery. We noted good functional prognosis in patients capable of ambulation within seven days and in patients who could survive longer than three months after the surgery. Conclusions: In various cancer patients with neoplastic spinal cord compression, skeletal instability as the primary impairment is a good indication for MISt, as the patients showed early ambulation with improved BI scores.
Using behavioral insights to design implementation strategies in public mental health settings: a qualitative study of clinical decision-making
Authors: Last Briana Shiri , Schriger Simone H , Timon Carter E. , Frank Hannah E , Buttenheim Alison M. , Rudd Brittany N , Fernandez-Marcote Sara , Comeau Carrie , Shoyinka Sosunmolu , Beidas Rinad S. 
Published: April 2020

Abstract
Background : Most studies evaluating the barriers and facilitators to evidence-based practice (EBP) implementation tend to overrely on stakeholder perspectives; underuse theory; and evaluate implementation of entire packages or protocols rather than specific, essential components of EBPs. These approaches make it challenging to generate implementation strategies that mechanistically target the core causes of behavior change. The present study seeks to leverage behavioral insights to identify factors that affect clinical decision-making surrounding the use of the trauma narrative (TN), the most active component of trauma focused-cognitive behavioral therapy (TF-CBT)—the gold standard EBP for youth with posttraumatic stress disorder—and to generate implementation strategies informed by these insights. Methods : Through semi-structured qualitative interviews, we sought the perspectives of trained TF-CBT therapists working in community mental health settings across the city of Philadelphia ( n =17) surrounding their clinical decision-making around when and why they use TNs. We used an iterative process of structured brainstorming and rapid validation informed by behavioral insights to uncover the barriers and facilitators to TN use and to generate implementation strategies using the “Easy Attractive Social Timely” (EAST) framework to increase TN implementation. Results : We generated and validated 9 hypothesized barriers to and facilitators of implementation of the trauma narrative that mapped onto 18 behavioral insights. Hypothesized barriers linked therapist perspectives (i.e., the barriers and facilitators they described) and behavioral insights to design 9 implementation strategies. Conclusions : The study responds to the growing need to identify implementation barriers and facilitators and to design implementation strategies that are informed by stakeholder perspectives, causal theories and that target specific components of EBPs. Our results reveal that behavioral insights of TN implementation can generate strategy designs. In the future, we will test the implementation strategies we designed to evaluate whether this process of developing implementation strategies is effective.