bluuebunny/crossref_metadata_2025_split huggingface dataset:
Are There Effects of Antiretroviral Usage, Caregiver Stigma, Caregiver Burden, and Caregiver Coping on the Quality of Life of Indonesian Children with HIV/AIDS?
Authors: Putera Azwin Mengindra , Irwanto Irwanto , Maramis Margarita Maria 
Published: May 2020

Abstract
Background

Antiretroviral (ARV) therapy has changed human immunodeficiency virus infections and acquired immune deficiency syndrome (HIV/AIDS) into chronic medical conditions. These conditions affect the quality of life of Indonesian children with HIV/AIDS. The aimed was analyzing the influence of ARV usage, caregiver stigma, caregiver burden, and caregiver coping on the quality of life of Indonesian children with HIV/AIDS.
Methods

Participants in this study were caregiver for Indonesian children with HIV/AIDS. This study measured ARV usage, caregiver stigma, caregiver burden, caregiver coping, and quality of life in Indonesian children with HIV/AIDS. Measurement data were analyzed using logistic regression test and ANOVA, test with p < 0.05.
Results

HIV/AIDS Indonesian children aged 7.7 ± 3.68 years, with an average participant age of 40.2 ± 11.26 years. Most participants were biological parents of Indonesian children with HIV/AIDS (64.2%) and female (56.6%). Participants stated that all Indonesian children with HIV/AIDS tend to keep their health status confidential. Most HIV/AIDS Indonesian children regularly used ARV (83.0%) and had a good quality of life (71.7%; β = 0.683; p < 0.001). Caregiver stigma value was 21.7 ± 2.06, with a median value of 22 (15–26; β = 0.064; p = 0.548). Participants had moderate and mild caregiver burden, with percentage of 50.9% and 49.1%, respectively (β = 0.058; p = 0.593). Caregiver coping value was 77.2 ± 7.30 (β = -0.196; p = 0.049).
Conclusions

There is an influence of the use of ARV drugs and caregiver coping with the quality of life of Indonesian children with HIV/AIDS. Regular ARV use improves quality of life, while the higher the caregiver coping, the lower the quality of life.
Bacterial Community Research of Migratory Bird in Chifeng, Neimeng via High-Throughput Sequencing
Authors: zheng Lin , Zhu Ling-Wei , Guan Jia-yao , Wang Ying , Jing Jie , Liang Bing , Ji Xue , Chen Ping , Guo Xue-Jun 
Published: May 2020

Abstract
Background: In August 2018, a large number of migratory birds died in Chifeng, Neimeng. We were entrusted by local animal disease prevention and control center to collect the migratory bird epidemic materials and their living environmental water, and in 2019, we collect the local migratory bird stool and their living environmental water again. The bacterial communities in migratory bird epidemic materials, water and aquatic plants are profifiled by high-throughput sequencing of the V3–V4 hypervariable region of 16S rDNA gene.Results: We found that the dominant phylum between migratory bird epidemic materials, water and aquatic plant were Proteobacteia, Bacteroidetes, Firmicutes, Fusobacteria and Verrucomicrobia in 2018. One year later, we found that the dominant phylum between migratory bird stool, water and aquatic plant were Proteobacteia, Firmicutes, Cyanobacteria, Bacteroidetes. The relative abundance among bacterial phylum notably differed between two years. The relative abundance of Fusobacteria and Verrucomicrobia were higher in samples in 2018, while that of Cyanobacteria was higher in water, 2019. The relative abundance of Fusobacteria in migratory bird epidemic materials began to decline in the later period over time. At genus level, the relative abundance of Vibrio, Clostridium and other patnogenic bacteria decreased markedly and disappeared in 2019. The salt content and pH show a downward trend.Conclusions: Differences in diet and geographical location can lead to diversification in migratory bird intestinal flora. It is necessary to pay attention to diversification in intestinal flora of migratory birds, especially the abundance of vibrio in intestine. The overall structure of intestinal flora and relative abundance changes of various species are showed intuitively by 16S rDNA amplicon. But comparing to PCR which using specific primers, the accuracy and sensitivity are relatively poor. In the case of purposeful detection, it can be used in combination with both 16S rDNA amplicon and PCR which using specific primers. They are more accurately, because of supplement each other.
Predicting Target Profiles with Confidence as a Service using Docking Scores
Authors: Ahmed Laeeq , Alogheli Hiba , Arvidsson Staffan , Alvarsson Jonathan , Berg Arvid , Larsson Anders , Schaal Wesley , Laure Erwin , Spjuth Ola 
Published: May 2020

Abstract
Background Identifying and assessing ligand-target binding is a core component in early drug discovery as one or more unwanted interactions may be associated with safety issues. Contributions We present an open-source, extendable web service for predicting target profiles with confidence using machine learning for a panel of 7 targets, where models are trained on molecular docking scores from a large virtual library. The method uses conformal prediction to produce valid measures of prediction efficiency for a particular confidence level. The service also offers the possibility to dock chemical structures to the panel of targets with QuickVina on individual compound basis. Results The docking procedure and resulting models were validated by docking well-known inhibitors for each of the 7 targets using QuickVina. The model predictions showed comparable performance to molecular docking scores against an external validation set. The implementation as publicly available microservices on Kubernetes ensures resilience, scalability, and extensibility.
Polyhydroxyalkanoates (PHAs) from dairy wastewater effluent: bacterial accumulation, structural characterization and physical properties
Authors: Pagliano Giorgia , Gugliucci Wanda , Torrieri Elena , Piccolo Alessandro , Cangemi Silvana , Giuseppe Fabio A Di , Robertiello Alessandro , Faraco Vincenza , Pepe Olimpia , Ventorino Valeria 
Published: May 2020

Abstract
Background to establish bioplastics the real alternative to conventional plastics, high production costs have to be constrained by using different kind of wastewater streams as organic substrate and novel microbial strains as accumulating bacteria with high performance. Volatile fatty acids (VFAs) from effluent of dairy wastewater biodigestion represent a new and cheap feedstock used in this study for biopolymers production through microbial processes.Results Cupriavidus necator DSM 13513 was particularly able to accumulate PHAs operating in fed-batch mode by limiting oxygen level together with intermittent feeding of carbon source with the maximum PHB accumulated in 48 h without compromising the microbial growth. The complex VFA mixture from digestate did not influence the PHA homopolymer accumulation. In fact, structural characterization by NMR analysis revealed the 3-hydroxybutirrate synthesis (PHB) by C. necator DSM 13513 grown with these different VFAs mixtures. Moreover, the bioplastic disk obtained C. necator DSM 13513 cells grown on VFA from digested dairy waste effluent, presented good thermic properties and low affinity to water.Conclusions overall results making the digested dairy waste effluent suitable for PHB production for specific bio-based industrial applications.
A prognostic model for predicting recurrence-free survival in hepatocellular carcinoma patients
Authors: Wang Wenhua , Wang Lingchen , Xie Xinsheng , Yan Yehong , Li Yue , Lu Quqin 
Published: May 2020

Abstract
Hepatocellular carcinoma (HCC) remains the most frequent liver cancer, accounting for approximately 90% of primary liver cancers worldwide. The recurrence-free survival (RFS) of HCC patients is a critical factor in devising a personal treatment plan. Thus, it is necessary to accurately forecast the prognosis of HCC patients in clinical practice. Using the The Cancer Genome Atlas （TCGA） dataset, we identified genes that are associated with RFS. A robust likelihood-based survival modeling approach was used to select the best genes for the prognostic model. Then, the GSE76427 dataset was used to evaluate the prognostic model’s effectiveness. We identified 1331 differentially expressed genes associated with RFS. Seven of these genes were selected to generate the prognostic model. Validation in both the TCGA cohort and the GEO cohort demonstrated that the 7-gene prognostic model has the capability of predicting the RFS of HCC patients. Meanwhile, the result of multivariate Cox regression showed that the 7-gene prognostic model could work as an independent prognostic factor. In addition, according to the time dependent ROC curve, the 7-gene prognostic model performed better in predicting the RFS of the training set and the external validation dataset than the classical TNM staging and BCLC. What’s more, these seven genes were found to be related to the occurrence and development of liver cancer by exploring other three databases. Our study identified a seven-gene signature for HCC RFS prediction that is a novel and convenient prognostic tool. The seven genes might provide potential target genes for metabolic therapy and the treatment of HCC.
Test re-test reliability of single and multijoint strength properties in female Australian footballers
Authors: Kadlec Daniel , Jordan Matthew J. , Snyder Leanne , Alderson Jacqueline , Nimphius Sophia 
Published: May 2020

Abstract
Purpose
To examine the test re-test reliability of isometric maximal voluntary contractions (MVC) of hip adduction (ADDISO), hip abduction (ABDISO), and multijoint leg extension (SQUATISO) in recreationally trained female Australian footballers.
Methods
Data were collected from 24 female Australian footballers (age: 22.6 ± 4.5 y; height: 169.4 ± 5.5 cm; body mass: 66.6 ± 8.0 kg; 4.5 ± 4.4 sport-specific training; 2.5 ± 2.0 resistance training years) from the same club on two non-consecutive days. Participants performed 3 isometric MVCs of ADDISO, ABDISO and SQUATISO. The SQUATISO was performed at 140° knee flexion with a vertical trunk position and ADDISO and ABDISO measures were performed in a supine position at 60° of knee flexion and 60° hip flexion. Reliability was assessed using paired T-tests and the intraclass correlation coefficient (ICC) with 95% confidence intervals (CI), typical error (TE) and coefficient of variation (CV%) with 95% CI.
Results
SQUATISO peak force (ICC: .95; CV%: 4.1), ABDISO for left, right and sum (ICC: .90 – .92; CV%: 5.0–5.7), and ADDISO for left right and sum (ICC: .86 − .91; CV%: 6.2–6.9) were deemed acceptably reliable based on predetermined criteria (ICC ≥ .8 and CV% ≤ 10).
Conclusion
SQUATISO, ABDISO and ADDISO tests demonstrated acceptable reliability for the assessment of peak force in recreational female Australian footballers, suggesting these assessments are suitable for muscle strength testing and monitoring adaptations to training.
Phylogeny and genetic relationship between hard ticks (Ixodidae) infesting cattle collected from selected areas of a wildlife-livestock interface ecosystem of Mikumi National Park, Tanzania.
Authors: Damian Donath , Damas Modester , Wensman Jonas Johansson , Berg Mikael 
Published: May 2020

Abstract
Background:

There is substantial increase in the number of tick species and tick-borne infectious agents in Tanzania. Due to their impact on human, livestock and wild animal health, increased knowledge of ticks is highly needed. So far, no published data on the phylogeny and the genetic distance between hard ticks collected from cattle is available in Tanzania.
Methods

Ticks from cattle in 9 wards, which lie at the border of Mikumi National Park, were collected in dry season (November and December) 2019. Morphological identification of ticks was initially performed to genus level. To identify ticks to species level, molecular analysis based on mitochondrion 16S rRNA gene was performed. The evolutionary relationships and genetic distance between ticks were determined using Maximum Likelihood and Kimura 2-parameter methods respectively.
Results

On the basis of morphology, two genera (Rhipicephalus and Hyalomma) were identified of the 630 adult ticks collected from a total of 252 cattle. Six species; R. microplus, R. evertsi, H. marginatum, H. rufipes, H. truncatum and H. turanicum were confirmed by BLASTn and phylogenetic analysis. The considerably mean and pairwise genetic distances for Rhipicephalus and Hyalomma genera were observed, whereas, the high overall mean and pairwise genetic distances were also recorded.
Conclusion

The presence of different (clusters) phylogroups and considerably mean and pairwise genetic distances observed, reflecting possible biological diversity of hard ticks present in the study area. The outcomes of this study will be useful in the planning of integrated control strategies for ticks and tick-borne diseases in Tanzania.
National Early Warning Score 2 (NEWS2) on admission predicts severe disease and in-hospital mortality from Covid-19 - A prospective cohort study
Authors: Myrstad Marius , Ihle-Hansen Håkon , Tveita Anders Aune , Andersen Elizabeth Lyster , Nygård Ståle , Tveit Arnljot , Berge Trygve 
Published: May 2020

Abstract
Background
There is a need for validated clinical risk scores to identify patients at risk of severe disease and to guide decision-making during the covid-19 pandemic. The National Early Warning Score 2 (NEWS2) is widely used in emergency medicine, but so far, no studies have evaluated its use in patients with covid-19. We aimed to study the performance of NEWS2 and compare commonly used clinical risk stratification tools at admission to predict risk of severe disease and in-hospital mortality in patients with covid-19.
Methods
This was a prospective cohort study in a public non-university general hospital in the Oslo area, Norway, including a cohort of all 66 patients hospitalised with confirmed SARS-CoV-2 infection from the start of the pandemic; 13 who died during hospital stay and 53 who were discharged alive. Data were collected consecutively from March 9th to April 27th 2020. The main outcome was the ability of the NEWS2 score and other clinical risk scores at emergency department admission to predict severe disease and in-hospital mortality in covid-19 patients. We calculated sensitivity and specificity with 95% confidence intervals (CIs) for NEWS2 scores ≥ 5 and ≥ 6, quick Sequential Organ Failure Assessment (qSOFA) score ≥ 2, ≥2 Systemic Inflammatory Response Syndrome (SIRS) criteria, and CRB-65 score ≥ 2. Areas under the curve (AUCs) for the clinical risk scores were compared using DeLong’s test.
Results
In total, 66 patients (mean age 67.9 years) were included. Of these, 23% developed severe disease. In-hospital mortality was 20%. Tachypnoea, hypoxemia and confusion at admission were more common in patients developing severe disease. A NEWS2 score ≥ 6 at admission predicted severe disease with 80.0% sensitivity and 84.3% specificity (Area Under the Curve (AUC) 0.822, 95% confidence interval (CI) 0.690–0.953). NEWS2 was superior to qSOFA score ≥ 2 (AUC 0.624, 95% CI 0.446–0.810, p < 0.05) and other clinical risk scores for this purpose.
Conclusion
NEWS2 score at hospital admission predicted severe disease and in-hospital mortality, and was superior to other widely used clinical risk scores in patients with covid-19.
α- Linolenic Acid Modulates Phagocytosis of Extracellular Tau and Induces Microglial Migration
Authors: Desale Smita Eknath , Chinnathambi Subashchandrabose 
Published: May 2020

Abstract
Background The seeding effect of extracellular Tau species is an emerging aspect to study the Tauopathies in Alzheimer’s disease. Tau seeds enhance the propagation of disease along with its contribution to microglia-mediated inflammation. Omega-3 fatty acids are known to exert the anti-inflammatory property to microglia by modulating cell membrane compositions. The immunomodulatory function of omega-3 fatty acids exerts anti-inflammatory properties to microglia. Owing to the imparted anti-inflammatory nature enhance phagocytosis and increased migration property has been observed in microglia. The dietary omega-3 fatty acids are found to change the lipid composition of the cell membrane that predominated many signaling cascades and by modulating specific receptor response. Thus the omega-3 fatty acids influence microglial response in Tauopathy. Methods N9 microglia cells were exposed to extracellular full-length Tau monomer and aggregates along with ALA (α- Linolenic acid) to study the internalization of exposed Tau. The degradation of internalized Tau studied with the endosomal markers Rab5 and Rab7. The final degradation step in phagocytosis has been studied with LAMP-2A as lysosomal markers. The changes in the rate of migration of microglia were assessed by wound-scratch assay along with Microtubule organizing center (MTOC) reorientation were studied after exposure of Tau and ALA as the property of highly migratory microglia. Results The increased phagocytosis of extracellular Tau monomer and aggregates has been observed upon ALA exposure to microglia cells. The intracellular degradation of internalized Tau species was targeted by early and late endosomal markers Rab5 and Rab7. The increased levels of LAMP-2A and colocalization with internalized Tau indicated the degradation via lysosome. These results indicate the degradation of internalized Tau species in the presence of ALA instead of getting accumulated in the cell. The enhanced migratory ability of microglia in the presence of ALA induces the MTOC repolarization and reduces the nuclear-centrosomal axis polarity and favorable anterior positioning of MTOC. Conclusions Tau seeds greatly contribute to the spread of disease, one way to reduce the spreading is to reduce the presence of extracellular Tau seed. Microglia could be influenced to reduce extracellular Tau seed with dietary fatty acids. Our results suggest that dietary fatty acids ALA significantly enhances phagocytosis and intracellular degradation of internalized Tau. Enhanced migration supports the phagocytosis process. Our approach provides insights into the beneficial role of ALA as an anti-inflammatory dietary supplement to treat AD.
Enhanced Load-Sharing Technology of Toroidal Drive
Authors: Dong Mengyu , Mao Meijiao , Yang Shiping 
Published: May 2020

Abstract
In the manufacture and assembly of a toroidal drive mechanism, errors caused have a great influence on the load sharing of the mechanism. To ensure favourable load-sharing characteristics of the mechanism and to compensate for inaccuracies in the manufacture and assembly of the mechanism parts, and based on the transmission characteristics and operating states characteristics of the mechanism, a floating oil film structure was designed to support the planetary gear. The elasticity and hydrodynamic effect of the floating oil film allow the planetary gear to achieve its own small floatation and produce a certain axial displacement, which compensates for the influence of error and achieves load sharing. To examine the effect of the floating oil film structure, the floating oil film bearing was simulated by FLUENT, the characteristics of the floating oil film were analyzed, and the stiffness and damping coefficients of the floating oil film were calculated. In ADAMS, the method of equivalent replacement of the floating oil film with spring damping was adopted to conduct a dynamic analysis on the toroidal drive mechanism with the floating oil film load-sharing structure, and the effectiveness and feasibility of this structure were verified by comparing the changes in transmission performance of the system over time.
Effect of Li Substitution on the Structure and Dielectric Constant of Bi0.5(Na⁠1-xLix)0.5TiO⁠3 (0 ≤ x ≤ 0.20) Solid Solution Series
Authors: Anjali K , Ajithkumar T G , Joy Pattayil 
Published: May 2020

Abstract
Bismuth sodium titanate Bi0.5Na0.5TiO3 (BNT) is a lead-free piezoelectric ceramic material with high Curie temperature. The effect of substitution of the smaller ion Li+ for the larger ion Na+ in Bi0.5(Na⁠1−xLix)0.5TiO⁠3 (0 ≤ x ≤ 0.20) on the structure of BNT is studied using powder X-ray diffraction (XRD) and Raman spectroscopy. Rietveld refinement analysis of the powder XRD patterns showed that all the compositions formed under monoclinic Cc space group, with the lattice parameters showing minor changes above x > 0.08. Raman spectral parameters such as position and intensity of a peak also showed a similar trend in the same Li concentration range with increasing Li content. A corresponding change in the variation of the dielectric constant with increasing Li content is observed suggesting a close correlation between the local structure and properties of the different compositions in the Bi0.5(Na⁠1−xLix)0.5TiO⁠3 solid solution series.
Autoantibodies against citrullinated-lipopolysaccharide binding protein as a novel biomarker in seronegative rheumatoid arthritis
Authors: Zhao Xiaozhen , Chen Yuling , Cheng Yongjing , Wen Wen , Li Yuhui , Jia Rulin , Deng Haiteng , Sun Xiaolin , Li Zhanguo 
Published: May 2020

Abstract
Background A specific feature of rheumatoid arthritis is the presence of citrullinated antigen and production of anti-citrullinated protein autoantibodies (ACPA) which can appear years prior to disease onset to trigger immune responses. In this study, the serum citrulline-containing antigens from RA patients were screened and the significance of the antibodies against citrullinated lipopolysaccharide binding protein (anti-cLBP) was studied. Methods Polypeptides isolated from the serum of patients with RA were identified by the Orbitrap high-precision proteomic technology. And then the citrulline-containing proteins was demonstrated.We synthesized citrullinated LBP peptide based on its richness and possible antigenity. The levels of anti-cLBP were determined in sera of 100 RA, 27 OA, 20 SLE and 50 healthy controls by indirect enzyme-linked immunosorbent assay (ELISA). Result A total of 11 citrulline-containing antigens were identified in proteins from sera of RA patients. By using citrullinated LBP, the antibodies (anti-cLBP) was detected in RA patients, healthy and disease controls. We found that the levels of anti-cLBP were significantly increased in RA patients. The sensitivity and specificity of anti-cLBP antibody were 28.00% and 95.92%, respectively. In anti-CCP-negative and RF-negative RA patients, the prevalences of anti-cLBP were 19.05% (4/21) and 20.59% (7/34), respectively. In addition, in RA patients of anti-CCP and RF-double negative, anti-cLBP was also detectable in 16.67% (3/18) of the patients. Further analysis of the clinical relevance, we found that anti-cLBP antibody was associated with disease activities in RA. It was noticed that the level of anti-cLBP was closely related with a high incidence of infection in patients with RA. Conclusion Anti-cLBP autoantibody is a novel biomarker in RA, especially in seronegative RA, and associated with disease severity.