bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Anatomy of Asian Palm Civet (Paradoxurus hermaphroditus) Brain in Timor Island
Authors: Amalo Filphin Adolfin , Selan Yulfia Nellymalik , Widi Antin Yeftanti Nugrahening , Rimu Andry Ndula 
Published: November 2019

Asian palm civet (Paradoxurus hermaphroditus) is native to some Asian regions, include Indonesia. Despite its wide spreading in those areas, study in regards to its anatomy is very few. This study is aimed to unveil the anatomy of Asian plam civet brain in Timor island. This study was performed in three Asian palm civets. The palm civets were anesthesized and humanely euthanazied using perfusion technique, and macroscopically observed for their brains anatomy. Results showed Asian palm civet consisted of three main parts which were cerebrum, cerebellum, and brain stem. Compared to brain of dog, sulci and gyri of the Asian palm civet is simpler, with paraflocculus lobes were merely located in dorsal and ventral parts of the brain. However, the vermis was more similar to dogs, and more developed compare to Reusettussp (Javanese codot). This suggests that the anatomical difference is a result of the different behavior, which are nocturnal animal and live on trees.
Composite and diachronous stratigraphic surfaces in low-gradient, transitional settings: The J-3 “unconformity” and the Curtis Formation, east-central Utah, U.S.A.
Authors: Zuchuat Valentin , Midtkandal Ivar , Poyatos-Moré Miquel , Da Costa Sigrid , Brooks Hannah Louise , Halvorsen Kristine , Cote Nathan , Sundal Anja , Braathen Alvar 
Published: November 2019

ABSTRACTUnconformities, by definition, correspond to erosional or nondepositional surfaces, which separate older strata below, from younger rocks above, encapsulating significant time gaps. However, recent studies have highlighted the composite nature of some unconformities, as well as their heterochronous and diachronous character, which challenge the use of such a definition in a four-dimensional dynamic environment. The J-3 Unconformity, separating the Middle Jurassic Entrada Sandstone from the Upper Jurassic Curtis Formation (and laterally equivalent units) in east-central Utah (USA), is laterally variable, generated by either erosion-related processes such as eolian deflation, and water-induced erosion, or by deformational processes. The J-3 Unconformity is a composite surface, formed by numerous processes that interacted and overlapped spatially and temporally. This study therefore demonstrates the heterochronous, diachronous, and non-unique nature of this surface interpreted as unconformity, where one process can be represented by varying expressions in the stratigraphic record, and conversely many processes may result in the same stratigraphic expression.
Experimental Study of Flooding both Low Salinity Water and Foam in Sandstone Reservoirs Bearing Heavy Crude Oil
Authors: Al-Saedi Hasan Naeem , Al-Jaberi Soura Kassim , Al-Bazzaz Waleed , Flori Ralph Emil 
Published: November 2019

Abstract
Many studies have been performed to increase oil recovery by using low salinity (LS) water flooding in the tertiary recovery mode due to its impact in altering the rock wettability towards being more water-wet, resulting in improved oil recovery. Foam was also utilized to enhance oil recovery due to its ability to increase residual oil mobility and sweep efficiency. In this study, we combine both LS water and foam to utilize the benefits of both EOR techniques.
To evaluate the combination EOR techniques, Berea sandstone cores were allotted for core-flooding experiments. The core-flooding results showed that an increased heavy oil recovery was obtained by injecting foam and lowering water salinity. The contact angle measurements agreed with the core-flooding observations.
Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils
Authors: Heinsch Stephen C , Hsu Suzie , Otto-Hanson Lindsey , Kinkel Linda , Smanski Michael 
Published: November 2019

Abstract
Bacteria within the genus Streptomyces remain a major source of new natural product discovery and as soil inoculants in agriculture where they promote plant growth and protect from disease. Recently, Streptomyces spp. have been implicated as important members of naturally disease-suppressive soils. To shine more light on the ecology and evolution of disease-suppressive microbial communities, we have sequenced the genome of three Streptomyces strains isolated from disease-suppressive soils and compared them to previously sequenced isolates. Strains selected for sequencing had previously showed strong phenotypes in competition or signaling assays. Results Here we present the de novo sequencing of three strains of the genus Streptomyces isolated from disease-suppressive soils to produce high-quality complete genomes. Streptomyces sp. GS93-23, Streptomyces sp. 3211-3, and Streptomyces sp. S3-4 were found to have linear chromosomes of 8.24 Mb, 8.23 Mb, and greater than 7.5 Mb, respectively. In addition, two of the strains were found to have large, linear plasmids. Each strain harbors between 26 and 38 natural product biosynthetic gene clusters, on par with previously sequenced Streptomyces spp.. We compared these newly-sequenced genomes with those of previously sequenced organisms. We see substantial natural product biosynthetic diversity between closely related strains, with the gain/loss of episomal DNA elements being a primary driver of genome evolution. Conclusions Long read sequencing data facilitates large contig assembly for high-GC Streptomyces genomes. While the sample number is too small for a definitive conclusion, we do not see evidence that disease suppressive soil isolates are particularly privileged in terms of numbers of BGCs. The strong sequence similarity between GS93-23 and previously isolated Streptomyces lydicus suggests that species recruitment may contribute to the evolution of disease-suppressive microbial communities.
A MYB-related Transcription Factor from Sheepgrass, LcMYB2, Promotes Seed Germination and Root Growth under Drought Stress
Authors: Zhao Pincang , Hou Shenglin , guo xiufang , Jia Junting , Yang Weiguang , Liu Zhujiang , Chen Shuangyan , Li Xiaoxia , Qi Dongmei , Liu Gongshe , Cheng Liqin 
Published: November 2019

Abstract
Background Drought is one of the most serious factors limiting plant growth and production. Sheepgrass can adapt well to various adverse conditions, including drought. However, during germination, sheepgrass young seedlings are sensitive to these adverse conditions. Therefore, the adaptability of seedlings is very important for plant survival, especially in plants that inhabit grasslands or the construction of artificial grassland. Results In this study, we found a sheepgrass MYB-related transcription factor, LcMYB2 that is up-regulated by drought stress and returns to a basal level after rewatering. The expression of LcMYB2 was mainly induced by osmotic stress and was localized to the nucleus. Furthermore, we demonstrate that LcMYB2 promoted seed germination and root growth under drought and ABA treatments. Additionally, we confirmed that LcMYB2 can regulate LcDREB2 expression in sheepgrass by binding to its promoter, and it activates the expression of the osmotic stress marker genes AtDREB2A, AtLEA14 and AtP5CS1 by directly binding to their promoters in transgenic Arabidopsis. Conclusions Based on these results, we propose that LcMYB2 improves plant drought stress tolerance by increasing the accumulation of osmoprotectants and promoting root growth. Therefore, LcMYB2 plays pivotal roles in plant responses to drought stress and is an important candidate for genetic manipulation to create drought-resistant crops, especially during seed germination.
Understanding the Healthcare Experiences and Needs of African Immigrants in the United States: A Scoping Review
Authors: Omenka Ogbonnaya Isaac , Watson Dennis P. , Hendrie Hugh C. 
Published: November 2019

Abstract
Background: Africans immigrants in the United States are the least-studied immigrant group, despite the research and policy efforts to address health disparities within immigrant communities. Although their healthcare experiences and needs are unique, they are often included in the “black” category, along with other phenotypically-similar groups. This process makes utilizing research data to make critical healthcare decisions specifically targeting African immigrants, difficult. The purpose of this Scoping Review was to examine extant information about African immigrant health in the U.S., in order to develop lines of inquiry using the identified knowledge-gaps. Methods: Literature published in the English language between 1980 and 2016 were reviewed in five stages: (1) identification of the question and (b) relevant studies, (c) screening, (d) data extraction and synthesis, and (e) results. Databases used included EBSCO, ProQuest, PubMed, and Google Scholar (hand-search). The articles were reviewed according to title and abstract, and studies deemed relevant were reviewed as full-text articles. Data was extracted from the selected articles using the inductive approach, which was based on the comprehensive reading and interpretive analysis of the organically emerging themes. Finally, the results from the selected articles were presented in a narrative format. Results: Culture, religion, and spirituality were identified as intertwined key contributors to the healthcare experiences of African immigrants. In addition, lack of culturally-competent healthcare, distrust, and complexity, of the U.S. health system, and the exorbitant cost of care, were identified as major healthcare access barriers. Conclusion: Knowledge about African immigrant health in the U.S. is scarce, with available literature mainly focusing on databases, which make it difficult to identify African immigrants. To our knowledge, this is the first Scoping Review pertaining to the healthcare experiences and needs of African immigrants in the U.S.
The impact of nurse staffing levels and nurse’s education on patient mortality in medical and surgical wards: an observational multicentre study.
Authors: Haegdorens Filip , Bogaert Peter Van , De Meester Koen , Monsieurs Koen 
Published: November 2019

Abstract
BACKGROUND
Growing evidence indicates that improved nurse staffing in acute hospitals is associated with lower hospital mortality. Current research is limited to studies using hospital level data or without proper adjustment for confounders which makes the translation to practice difficult.
METHOD
In this observational study we analysed retrospectively the control group of a stepped wedge randomised controlled trial concerning 14 medical and 14 surgical wards in seven Belgian hospitals. All patients admitted to these wards during the control period were included in this study. Pregnant patients or children below 17 years of age were excluded. In all patients, we collected age, crude ward mortality, unexpected death, cardiac arrest with Cardiopulmonary Resuscitation (CPR), and unplanned admission to the Intensive Care Unit (ICU). A composite mortality measure was constructed including unexpected death and death up to 72 hours after cardiac arrest with CPR or unplanned ICU admission.
Every four months we obtained, from 30 consecutive patient admissions across all wards, the Charlson comorbidity index. The amount of nursing hours per patient days (NHPPD) were calculated every day for 15 days, once every four months. Data were aggregated to the ward level resulting in 68 estimates across wards and time. Linear mixed models were used since they are most appropriate in case of clustered and repeated measures data.
RESULTS
The unexpected death rate was 1.80 per 1000 patients. Up to 0.76 per 1000 patients died after CPR and 0.62 per 1000 patients died after unplanned admission to the ICU. The mean composite mortality was 3.18 per 1000 patients. The mean NHPPD and proportion of nurse Bachelor hours were respectively 2.48 and 0.59. We found a negative association between the nursing hours per patient day and the composite mortality rate adjusted for possible confounders (B= -2.771, p=0.002). The proportion of nurse Bachelor hours was negatively correlated with the composite mortality rate in the same analysis (B= -8.845, p=0.023). Using the regression equation, we calculated theoretically optimal NHPPDs.
CONCLUSIONS
This study confirms the association between higher nurse staffing levels and lower patient mortality controlled for relevant confounders.
Men’s grief following pregnancy loss and neonatal loss: A systematic review and emerging theoretical model
Authors: Obst Kate , Due Clemence , Oxlad Melissa , Middleton Philippa 
Published: November 2019

Abstract
Background: Emotional distress following pregnancy loss and neonatal loss is common, with enduring grief occurring for many parents. However, little is known about men’s grief, since the majority of existing literature and subsequent bereavement care guidelines have focused on women. To develop a comprehensive understanding of men’s grief, this systematic review sought to summarise and appraise the literature focusing on men’s grief following pregnancy loss and neonatal loss.
Methods: A systematic review was undertaken with searches completed across four databases (PsycINFO, PubMed, Embase and CINAHL). These were guided by two research questions: 1) what are men’s experiences of grief following pregnancy/neonatal loss; and 2) what are the predictors of men’s grief following pregnancy/neonatal loss? Eligible articles were qualitative, quantitative or mixed methods empirical studies including primary data on men’s grief, published between 1998 and October 2018. Eligibility for loss type included any definition of miscarriage or stillbirth, termination of pregnancy for nonviable foetal anomaly, and neonatal death up to 28 days after a live birth.
Results: A final sample of 46 articles were identified, of which 26 were qualitative, 19 quantitative, and one mixed methods. Findings indicate that men’s grief experiences are highly varied, and current grief measures may not capture all of the complexities of grief for men. Qualitative studies identified that in comparison to women, men may face different challenges including expectations to support female partners, and a lack of social recognition for their grief and subsequent needs. Men may face double-disenfranchised grief in relation to the pregnancy/neonatal loss experience.
Conclusion: There is a need to increase the accessibility of support services for men following pregnancy/neonatal loss, and to provide recognition and validation to their experiences of grief. To refine an emerging socio-ecological model of men’s grief, cohort studies are required among varied groups of bereaved men to confirm grief-predictor relationships.
The effect of the laparoscopic primary suture of the common bile duct in elderly patients: a retrospective study
Authors: Liu Jinheng , Wang Yanting , Liu Xubao , Yin Sineng 
Published: November 2019

Abstract
Background Traditional surgery treatment strategies for elderly patients who were diagnosed as choledocholithiasis combined with cholecystolithiasis include laparoscopic choledocholithotomy, cholecystectomy, and T-tube drainage. However, long-term indwelling T-tube drainage will cause pain and other complications. This study was designed to compare the primary suture of the common bile duct and the T-tube drainage after laparoscopic choledochotomy to study the merits and demerits between primary suture and T-tube drainage in elderly patients. Methods Eighty-five patients were screened from January 2017 to January 2018. All patients were performed laparoscopic surgery. Fifty-six cases were the primary suture group (n=56). Twenty-nine cases were the T-tube group (n=29). Preoperative baseline characteristics, intraoperative and postoperative characteristics of the two groups were compared. Results There were no differences of baseline characteristics in both groups (P>0.05). Compared with the T-tube group, the postoperative total drainage volume on the first day and patients of residual stones were fewer, and all drainage tube indwelling time was shorter in the primary suture group(P﹤0.05). And there were statistically significant differences in postoperative total drainage volume on the first day, all drainage tube indwelling time, postoperative total bilirubin and residual stones between the two groups (P<0.05). There were no statistically significant differences of the other intraoperative and postoperative characteristics(P>0.05). There were no pressure sores, hypostatic pneumonia, deep vein thrombosis, serious complications of heart, lung and brain and even death in both groups. Conclusions With accurate preoperative risk assessment and strict treatment of basic diseases in elderly patients, and the stones were removed in laparoscopic surgery, the primary suture in elderly patients is feasible, safe, and valid.
GINGIVAL THICKNESS ASSESSMENT AT MANDIBULAR INCISORS OF ORTHODONTIC PATIENTS WITH ULTRASOUND AND CONE-BEAM CT. A CROSS-SECTIONAL STUDY
Authors: Kloukos Dimitrios , Kakali Lydia , Koukos George , Sculean Anton , Stavropoulos Andreas , Katsaros Christos 
Published: November 2019

Abstract
Background Quantitative and qualitative analysis of several periodontal parameters plays an important role in several dental procedures. Aim of the current study was to assess gingival thickness (GT) at mandibular incisors of orthodontic patients with two methods and determine how these methods are compared to each other when assessing periodontal anatomy through soft tissue thickness.Methods The sample consisted of 40 consecutive adult orthodontic patients. GT was measured at both central mandibular incisors, mid-facially on the buccal aspect, 2mm apically to the free gingival margin with two methods: a) clinically with an Ultrasound device (USD) and b) radiographically with Cone Beam Computed Tomography (CBCT).Results CBCT measurements were consistently higher than USD measurements, with the difference ranging from 0.13 mm to 0.21 mm. No difference was noted between the repeated CBCT measurements at the right central incisor (Bias= 0.05 mm; 95% CI= -0.01, 0.11, p=0.104). Although the respective results for the left incisor indicated, statistically, that the measurements were not exactly replicated, the magnitude of the point estimate was small and not clinically significant (Bias= 0.06 mm; 95% CI= 0.01, 0.11, p=0.014). Small differences between CBCT measurements made by the 2 examiners at the left central incisor (bias= 0.06 mm, 95% CI= 0.01, 0.11, p=0.014) were detected. However, this difference was minor, and again, not clinically significant. The respective analysis on the right incisor showed no significant difference (bias= 0.05 mm, 95% CI= -0.01, 0.11, p=0.246).Conclusions Present data indicate that CBCT measurements were highly reproducible and yielded greater values compared to USD measurements.
Identifying risk factors of anemia among women of reproductive age in Rwanda to inform designing better interventions – a secondary data analysis, cross-sectional study using the Rwanda Demographic and Health Survey (RDHS) data
Authors: Hakizimana Dieudonne , Nisingizwe Marie Paul , Logan Jenae , Wong Rex 
Published: November 2019

Abstract
Background Anemia among Women of Reproductive Age (WRA) continues to be among the major public health problems in many developing countries including Rwanda where it was increased comparing 2015 to 2010 Rwanda Demographic and Health Survey (RDHS) reports. A thorough understanding of the its risk factors is necessary to design interventions. However, to the best of our knowledge, no study with national representation assessing anemia risk factors among WRA has been conducted in Rwanda. Therefore, this study aims to identify anemia risk factors among WRA in Rwanda. Methods This was a quantitative, cross-sectional study using secondary data from the 2014-2015 RDHS data. The study population consisted of 6680 WRA who were tested for anemia during the survey. Anemia was defined as having a hemoglobin level equal or below to 10.9 g/dl for a pregnant woman, and hemoglobin level equal or below to 11.9 g/dl for a non-pregnant woman. Pearson’s chi-squared test and multiple logistic regression were conducted for bivariate and multivariable analysis respectively. We reported Odds Ratio (OR), 95% Confidence Intervals (CI) and p-values. Results The overall prevalence of anemia among WRA was 19.2% (95% CI: 18.0 - 20.5). After controlling for other variables, four factors were found associated with lower odds of anemia, they are being obese (OR: 0.61, 95% CI: 0.40 - 0.91), being in rich category (OR: 0.74, 95% CI: 0.63 - 0.87), sleeping under a mosquito net (OR: 0.85, 95% CI: 0.74 - 0.98), and using hormonal contraceptives (OR: 0.61, 95% CI: 0.50 - 0.73). Four factors associated with higher odds of anemia were being underweight (OR: 1.39, 95% CI: 1.09 - 1.78), using an Intra Uterus Device (OR: 1.98, 95% CI: 1.05 - 3.75), and living in the Southern province (OR: 1.45, 95% CI: 1.11 - 1.89) or in the Eastern province (OR: 1.41, 95% CI: 1.06 - 1.88). Conclusion Anemia continues to pose public health challenges; novel public health interventions should consider geographic variations, improve women economic status, and strengthen iron supplementation especially for Intrauterine Device users. Additionally, given the association between anemia and malaria, interventions to prevent malaria should be enhanced.
Archaic mitochondrial DNA inserts in modern day nuclear genomes
Authors: Bücking Robert , Cox Murray P , Hudjashov Georgi , Saag Lauri , Sudoyo Herawati , Stoneking Mark 
Published: November 2019

Abstract
Background:
Traces of interbreeding of Neanderthals and Denisovans with modern humans in the form of archaic DNA have been detected in the genomes of present-day human populations outside sub-Sahara Africa. Up to now, only nuclear archaic DNA has been detected in modern humans; we therefore attempted to identify archaic mitochondrial DNA (mtDNA) residing in modern human nuclear genomes as nuclear inserts of mitochondrial DNA (NUMTs).
Results:
We analysed 221 high-coverage genomes from Oceania and Indonesia using an approach which identifies reads that map both to the nuclear and mitochondrial DNA. We then classified reads according to the source of the mtDNA, and found one NUMT of Denisovan mtDNA origin; analysis of the flanking region suggests that this insertion is more likely to have happened in a Denisovan individual and introgressed into modern humans with the Denisovan nuclear DNA, rather than in a descendant of a Denisovan female and a modern human male.
Conclusions:
Here we present our pipeline for detecting introgressed NUMTs in next generation sequencing data that can be used on genomes sequenced in the future. Further discovery of such archaic NUMTs in modern humans can be used to detect interbreeding between archaic and modern humans and can reveal new insights into the nature of such interbreeding events.