bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and Associated Factors of Psychological Distress Among Nurses in public Hospitals, Southwest, Ethiopia: A Cross-Sectional Study Design
Authors: Belay Alemayehu Sayih , Guangul Melak Menberu , Asmare Wondwossen Niguse 
Published: July 2020

Abstract
Background: Psychological distress is broadly defined as a state of emotional suffering characterized by symptoms of depression and anxiety combined by other somatic symptoms like; insomnia, headaches, and lack of energy that is likely to vary across different areas. Therefore, this study aimed at assessing the prevalence of psychological distress and its’ associated factors among nurses in public hospitals, Southwest Ethiopia, 2017/18.Method: An institutional-based cross-sectional study was conducted in public hospitals, Southwest Ethiopia from February 1st, 2018 to April 1st, 2018. The total number of nurses who fulfill the inclusion criteria were enrolled. Data was collected using the predesigned tool like Self-Reporting Questionnaire version 20 (SRQ 20). Data were entered using EPI INFO version 7 and was exported to statistical packages for social science (SPSS) version 21.0 for analysis. Bivariate and multivariate logistic regression was employed and odds ratio with 95% confidence interval was used to assess the degree of association between variables. Variables with a P-value of less than 0.05 were considered as statistically significant.Result: A total of 282 eligible nurses were enrolled in the study with mean age of 28.71 [SD ±7.047]. The prevalence of psychological distress among nurses was 78(27.7%). Predictor variables like; job title [AOR= 10.13, 95% CI (1.85, 55.41)], work experience [AOR= 4.98, 95% CI (1.59, 15.568)], interaction with staffs [AOR= .305, 95% CI (.117, .796)], fatigue [AOR= 2.54, 95% CI (1.072, 6.02)], social support [AOR= .189, 95% CI (.075, .474)], perfectionism [AOR= 5.697, 95% CI (1.449, 22.39)] and insomnia [AOR= 3.82, 95% CI (1.52, 9.579)] were found to be strong predictor variables for psychological distress.Conclusion: This study revealed that a considerable proportion of nurses had psychological distress. Therefore, strong social support, upgraded working status, and good interaction with staff should be encouraged among nurses working in the health setting to cope up with different stressors.
Prevalence of Depression, Anxiety, Delirium, and Post-Traumatic Stress Disorder Among COVID-19 Patients: Protocol for A Living Systematic Review
Authors: Shi Jiyuan , Li Yuanyuan , Zhao Liang , Yan Meili , Niu MingMing , Chen Yamin , Song Ziwei , Ya Gao , Tian Jinhui 
Published: July 2020

Abstract
BackgroundPrevious studies on the impact of COVID-19 on the mental health of the patients has been limited by the lack of relevant data. With the rapid and sustained growth of the publications on COVID-19 research, we will perform a living systematic review (LSR) to provide comprehensive and continuously updated data to explore the prevalence of depression, anxiety, delirium, and post-traumatic stress disorder (PTSD) among COVID-19 patients.MethodsWe will perform a comprehensive search of the following databases: Cochrane library, PubMed, Web of Science, Embase, and Chinese Biomedicine Literature to identify relevant studies. We will utilize different tools to examine the bias risks (quality) regarding studies of varying design types, such as the revised Cochrane risk-of-bias tool (RoB 2) for randomized controlled trials (RCT), the Newcastle-Ottawa Scale (NOS) for cohort and case-control studies, etc. The literature searches would be updated every month. We will perform meta-analysis if any new eligible studies or data are obtained and resubmit an updated systematic review if any change in outcomes and heterogeneity is determined after the addition of the new studies. There will be no restrictions on language or year of publication.DiscussionThis LSR would provide an in-depth and up-to-date summary of the psychological impact of COVID-19 diagnosis and treatment on the patients. Systematic review registrationPROSPERO CRD42020196610
The Seal Louse (Echinophthirius horridus) in the Dutch Wadden Sea: Investigation of Vector-Borne Pathogens and Morphological Adaptations
Authors: Hirzmann Jörg , Ebmer David , Contreras Guillermo J. Sánchez , Rubio-García Ana , Magdowski Gerd , Gärtner Ulrich , Taubert Anja , Hermosilla Carlos 
Published: July 2020

Abstract
Background: Belonging to the anopluran family Echinophthiriidae, Echinophthirius horridus, the seal louse, has been reported to parasitize a broad range of representatives of phocid seals. So far, only few studies focused on vector function of echinophthiriid lice and knowledge on their role in pathogen transmission is still scarce. The current study aims to investigate the role of E. horridus in vector-borne diseases of seals in the Dutch Wadden Sea and to attribute to its morphological features of environmental adaptation.Methods: More than 1200 E. horridus seal lice were collected from 54 harbour seals (Phoca vitulina) and one grey seal (Halichoerus grypus) during their rehabilitation period in the Sealcentre Pieterburen, the Netherlands. DNA was extracted from pooled seal lice of individual seals for molecular detection of the seal heartworm Acanthocheilonema spirocauda, the rickettsial intracellular bacterium Anaplasma phagocytophilum, and Mycoplasma spp. using PCR assays. In addition E. horridus-adult and -eggs were analysed by scanning electron microscopy (SEM).Results: Seal lice from 35% of the harbour seals (19/54) and from the grey seal proved positive for A. spirocauda. The seal heartworm was molecularly characterised and phylogenetically analysed for the first time (rDNA, cox1). A nested PCR was developed for the cox1 gene to detect A. spirocauda stages in seal lice. A. phagocytophilum and a Mycoplasma species previously identified from a patient with disseminated ‘seal finger’ mycoplasmosis were detected the first time in seal lice. SEM analyses of E. horridus-adults and -eggs brought out more clearly unique morphological features, such as ‘lock-like’ claws, setae-covered cuticle as well as vaulted nit lids carrying micropyles for respiration, which all demonstrate the adaption of this ectoparasite to its semiaquatic host and the marine environment.Conclusions: Our findings support the vector role of seal lice in transmission of A. spirocauda, Mycoplasma spp. and A. phagocytophilum and presented more detailed images of their morphological adaptations to the semiaquatic lifestyle of their hosts. As the vector-borne pathogens might have detrimental effects on the health of seal populations further epidemiological investigations on infections due to these pathogens in seals should be conducted.
Impact of CKLF-like MARVEL Transmembrane Domain Containing 6 (CMTM6) Expression in Gastric Cancer
Authors: Nishi M , Shimada Mitsuo , Yoshikawa Kozo , Higashijima Jun , Tokunaga Takuya , Kashihara Hideya , Takasu Chie , Eto Shohei , Yoshimoto Toshiaki 
Published: July 2020

Abstract
Background: CKLF-like MARVEL transmembrane domain containing 6 (CMTM6) is the master regulator of programmed cell death-ligand 1(PD-L1). We aimed to clarify the significance of CMTM6 expression in gastric cancer (GC). Methods: A total of 105 patients who had undergone curative surgical resection for stage II/III GC at Tokushima University Hospital were included in this study. The expression of CMTM and PD-L1 was examined by immunohistochemistry. Additionally, the relationship of each expression level to several prognostic factors was examined using univariate and multivariate analyses. Results: CMTM6 was not positively correlated with any of the factors examined. The overall survival (OS) rates were significantly poorer in the CMTM6 high-expression group than in the CMTM low-expression group (5-year OS: 57.2% vs. 79.2%, respectively; p<0.05). Disease-free survival (DFS) was significantly poorer in the CMTM high-expression group than in the CMTM6 low-expression group (5-year DFS: 52.8% vs. 72.4%, respectively; p=0.20). Multivariate analysis confirmed CMTM6 expression as an independent prognostic factor in DFS (p<0.05). CMTM6 expression tended to be correlated with PD-L1 expression (p=0.07), and PD-L1 expression was positively correlated with PD-1 expression (p<0.05).Conclusions: CMTM6 is associated with a poor prognosis and immunotolerance through PD-L1 in GC.
Mass Spectrometric (MS) Analysis of Proteins and Peptides
Authors: Jayathirtha Madhuri , Dupree Emmalyn J. , Manzoor Zaen , Larose Brianna , Sechrist Zach , Neagu Anca-Narcisa , Petre Brindusa Alina , Darie Costel C. 
Published: July 2020

:
The human genome is sequenced and comprised of ~30,000 genes, making humans just a
little bit more complicated than worms or flies. However, complexity of humans is given by proteins
that these genes code for because one gene can produce many proteins mostly through alternative
splicing and tissue-dependent expression of particular proteins. In addition, post-translational modifications
(PTMs) in proteins greatly increase the number of gene products or protein isoforms. Furthermore,
stable and transient interactions between proteins, protein isoforms/proteoforms and PTM-ed
proteins (protein-protein interactions, PPI) add yet another level of complexity in humans and other
organisms. In the past, all of these proteins were analyzed one at the time. Currently, they are analyzed
by a less tedious method: mass spectrometry (MS) for two reasons: 1) because of the complexity of
proteins, protein PTMs and PPIs and 2) because MS is the only method that can keep up with such a
complex array of features. Here, we discuss the applications of mass spectrometry in protein analysis..
Promising Anti-stroke Signature of Voglibose: Investigation through In- Silico Molecular Docking and Virtual Screening in In-Vivo Animal Studies
Authors: Shah Pooja , Chavda Vishal , Patel Snehal , Bhadada Shraddha , Ashraf Ghulam Md. 
Published: July 2020

Background:
Postprandial hyperglycemia considered to be a major risk factor for
cerebrovascular complications.

Objective:
The current study was designed to elucidate the beneficial role of voglibose via in-silico in
vitro to in-vivo studies in improving the postprandial glycaemic state by protection against strokeprone
type 2 diabetes.

Material and Methods:
In-Silico molecular docking and virtual screening were carried out with the
help of iGEMDOCK+ Pymol+docking software and Protein Drug Bank database (PDB). Based on the
results of docking studies, in-vivo investigation was carried out for possible neuroprotective action.
T2DM was induced by a single injection of streptozotocin (90mg/kg, i.v.) to neonates. Six weeks after
induction, voglibose was administered at the dose of 10mg/kg p.o. for two weeks. After eight weeks,
diabetic rats were subjected to middle cerebral artery occlusion, and after 72 hours of surgery,
neurological deficits were determined. The blood was collected for the determination of serum
glucose, CK-MB, LDH and lipid levels. Brains were excised for determination of brain infarct
volume, brain hemisphere weight difference, Na+-K+ ATPase activity, ROS parameters, NO levels,
and aldose reductase activity.

Results:
In-silico docking studies showed good docking binding score for stroke associated proteins,
which possibly hypotheses neuroprotective action of voglibose in stroke. In the present in-vivo study,
pre-treatment with voglibose showed a significant decrease (p<0.05) in serum glucose and lipid levels.
Voglibose has shown significant (p<0.05) reduction in neurological score, brain infarct volume, the
difference in brain hemisphere weight. On biochemical evaluation, treatment with voglibose produced
significant (p<0.05) decrease in CK-MB, LDH, and NO levels in blood and reduction in Na+-K+
ATPase, oxidative stress, and aldose reductase activity in brain homogenate.

Conclusion:
In-silico molecular docking and virtual screening studies and in-vivo studies in MCAo
induced stroke, animal model outcomes support the strong anti-stroke signature for possible
neuroprotective therapeutics.
Hungry Rice-based Biscuits: Effects on Some Biochemical Indices of Alloxan- induced Diabetic Adult Male Wistar Rats
Authors: Chikwendu Justina N. , Adeosun Florence F. , Onwurafor Eucharia U. , Udenta Elizabeth A. , Maduforo Aloysius N. 
Published: July 2020

Background:
High costs of hypoglycemic drugs and their side effects have caused an increase
in demand for the production of low glycemic plant foods and snacks from locally available
nutrient dense crops for diabetes management. Hungry rice is known for its high fiber content and
hypoglycemic properties.

Objectives:
The study investigated the effect of Hungry rice-based biscuits on some biochemical indices
of alloxan-induced diabetic Wistar rats.

Methods:
Four groups of six adult male Wistar rats each with a mean weight of 164.59 g were fasted
overnight and induced diabetes by a single intraperitoneal injection of alloxan at a dose of 150
mg/kg body weight and fed biscuits containing hungry rice/unripe plantain/cashew nut
(80:10:10%), hungry rice/unripe-plantain (80:20%), hungry rice/cashew nut (80:20%) or wheat
(100%) and water ad libitum for 21 days. The non-induced rats fed poultry growers’ mash serve as
the control. The rats’ body weight and food intake were evaluated while the lipid profile, liver enzyme
markers, and serum protein were assayed.

Results:
Hungry rice-based biscuits showed decreases in fasting blood glucose, AST, ALT, ALP,
TC, TG, and LDL-C and increases in HDL-C and total serum protein of rats. ALP and serum protein
were more in rats fed Wheat biscuit and 80% hungry rice-20% unripe plantain biscuit. The biscuit
containing 20% cashew nut showed a better desirable effect on all the parameters tested.

Conclusion:
The hungry rice-based-biscuits exhibited hypoglycemic and hypolipidemic potentials
with desirable effect on liver biomarkers and serum proteins; and may be good for diabetes and
weight management.
Naringenin and Ellagic Acid Reduce Tetrazolium Salt in the Absence of Cells
Authors: Tiwary Vaibhav , Sarkar Priyanka , Thirumurugan Kavitha 
Published: July 2020

Background:
Plant derived compounds, naringenin and ellagic acid might interfere in MTT (3-(4, 5-
Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay in the form of reducing the tetrazolium salt in the absence
of cells.

Objective:
Effect of naringenin and ellagic acid in MTT assay.

Methods:
MTT assay has been performed at different conditions like, varying the concentration of phytocompounds,
incubation time, and changing the media.

Results:
Ellagic acid at the concentration of 80 μg/ml showed maximum absorbance of 0.579 in
the presence of RPMI media (Roswell Park Memorial Institute Medium) that has 10% newborn
calf serum. Naringenin showed higher absorbance of 2.74 at 80 μg/ml in RPMI media that has 10%
newborn calf serum. Naringenin and Ellagic acid had better ability to reduce the MTT in the presence
of RPMI media than anhydrous ethanol.

Conclusion:
Naringenin reduced the MTT better than ellagic acid in RPMI-1640 medium. These results show the need to
be cautious of choosing MTT assay to measure the metabolic activity of cells treated with phytocompounds.
Gallic Acid Improved Amytryptiline Effect in Neuropathic Pain Induced by Partial Sciatic Nerve Ligation (PSNL) in Rats
Authors: Neerati Prasad , Enumula Vinay 
Published: July 2020

Aim and Backround:
Amytriptiline (AMY), an antidepressant, is used in the treatment
of neuropathic pain. However, with limited therapeutic benefits and side effects of AMY, it is not
helpful to the majority of the neuropathic pain patients. Gallic acid (GA) is a polyphenolic product
with potential antioxidant effects, useful in neuropathic pain.

Objective:
The purpose of the study was to investigate the improved neuropathic pain relief with
GA in combination with AMY in rats.

Methods:
Partial sciatic nerve ligation (PSNL) method is used for neuropathic pain induction in
rats. The rats were randomly divided into 6 groups (n = 8), and treated with drugs or vehicle once
daily for 15 days after the pain induction. The first group served as Normal control and received
normal saline (p.o), the second group served as Sham Control and received Normal Saline (p.o),
the third group served as Surgery Control and received normal saline (p.o), the fourth group received
AMY (25mg/kg, p.o), while the fifth group received GA (100mg/kg,p.o), and the sixth
group received both AMY and GA. Further behavioural studies for pain were carried out on days
1,4,7,10,13,16,19 and 22. Histological studies of sciatic nerves were carried out on days 1, 15 and
22, and on the 22nd day, all the remaining animals were sacrificed for the biochemical estimations
of the sciatic nerve tissues.

Results:
GA attenuates neuropathic pain better in combination with AMY by ameliorating the behavioral,
biochemical and histopathological signs and symptoms. This resultant effect is due to its
antioxidant, antinociceptive and anti-inflammatory actions. It also potentiates AMY antinociceptive
and antioxidant effects when given in combination in a continuous treatment.

Conclusion:
The results suggest that GA in combination with AMYattenuated the neuropathic
pain. Hence, the use of GA as an adjunctive with AMY in the neuropathic pain will be more therapeutically
beneficial; further studies on patients to confirm the results are warranted.
A Novel Static Path Planning Method for Mobile Anchor-Assisted Localization in Wireless Sensor Networks
Authors: Naguib Abdelhady M. , Ali Shahzad 
Published: July 2020

Background:
Many applications of Wireless Sensor Networks (WSNs) require awareness
of sensor node’s location but not every sensor node can be equipped with a GPS receiver for
localization, due to cost and energy constraints especially for large-scale networks. For localization,
many algorithms have been proposed to enable a sensor node to be able to determine its location by
utilizing a small number of special nodes called anchors that are equipped with GPS receivers. In
recent years a promising method that significantly reduces the cost is to replace the set of statically
deployed GPS anchors with one mobile anchor node equipped with a GPS unit that moves to cover
the entire network.

Objectives:
This paper proposes a novel static path planning mechanism that enables a single anchor node to follow a predefined static path while periodically broadcasting its current location coordinates to the nearby sensors. This new path type is called
SQUARE_SPIRAL and it is specifically designed to reduce the collinearity during localization.

Results:
Simulation results show that the performance of SQUARE_SPIRAL mechanism is better
than other static path planning methods with respect to multiple performance metrics.

Conclusion:
This work includes an extensive comparative study of the existing static path planning methods then presents a comparison of the proposed mechanism with existing solutions by doing extensive simulations in NS-2.
Warehousing Operation Optimization Model of One Storey New Dangerous Goods Warehouse Based on Warehousing Chain and Its Application
Authors: Li Xiaguang , Lin Xiefang , Zhang Fangwei , Tang Xufeng , Qiu Ruolin , Sun Xue 
Published: July 2020

Introduction:
In order to ensure the efficiency and cost of dangerous goods warehouse
under the premise of safety, this study takes the dangerous goods warehouse as the research object
and implements multitask for dangerous goods warehouse with two forklifts.

Methods:
This study takes traversal calculation, novel safety calculation formula and scheduling
scheme evaluation model as tools to research the forklift scheduling scheme of one-storey packed
dangerous goods warehouse.

Results:
Optimal scheme and allocation decision model are obtained through numerical simulation.
The innovation of this study is giving the safety formula of forklift operation in dangerous goods
warehouse and using numerical simulation to obtain the global optimal solution. Furthermore, this
study draws on the concept of the travel chain to propose the idea of warehousing chain. At the same
time, optimal schemes for multiple dangerous goods inbound and outbound the warehouse are studied.

Conclusion:
With the application of actual data in Shanghai Lingang dangerous goods warehouse,
this study combines with the simulation technology to verify the allocation model of warehousing
operations and forklift scheduling model. The validity and feasibility of the novel theory are also
verified.
A Companion App to Support Rheumatology Patients Treated with Certolizumab Pegol: Results From a Usability Study
Authors: Domańska Barbara , Vansant Stijn , Mountian Irina 
Published: July 2020

Background
Certolizumab pegol (CZP) is an anti-tumor necrosis factor drug approved for the treatment of multiple moderate to severe chronic inflammatory diseases. In the European Union, CZP is approved for administration by subcutaneous self-injection using a prefilled syringe, prefilled pen, or reusable electromechanical auto-injector (electronic device). CimplyMe is a companion app for use alongside CZP self-injection devices, designed to support CZP-treated patients self-managing their treatment and disease.

Objective
This study aimed to validate the usability of the companion app by demonstrating that tasks required for use can be performed successfully by intended end users.

Methods
We recruited 15 patients with moderate to severe rheumatoid arthritis, currently prescribed biologic treatment, and using apps on a smart phone. Patients were assessed on their ability to use the companion app in a setting designed to simulate a location where patients regularly administer biologic treatment. To assess the usability of the key features of the app, 8 critical and 3 noncritical tasks were designed. Patients’ success on each task was recorded through observations or knowledge-based questions. Successes with difficulty and use errors were also recorded. If a patient made a use error at the first attempt, a second attempt was allowed. Second-attempt use errors were recorded as a task failure.

Results
A total of 207 first attempts at the 14 components of the 8 critical tasks were evaluated (3 patients failed to complete one component); 178 (86.0%) critical tasks were successfully completed at the first attempt. The remaining first attempts comprised 16 (7.7%) successes with difficulty and 13 (6.3%) use errors, which had to be repeated. One critical task was not re-attempted by one patient due to time constraints; however, there were no use errors in the 12 completed second attempts. A total of 107 first attempts at the 3 noncritical tasks were made, all of which (107/107, 100.0%) were completed without use errors.

Conclusions
In simulated testing, patients were able to successfully use the companion app without formal training. This study suggests the companion app is easy to use and could help patients prescribed CZP better manage their treatment and disease.