bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety of Endoscopic Ultrasound-Guided Fine-Needle Aspiration for Pancreatic Solid Pseudopapillary Neoplasm Before Surgical Resection
Authors: Karsenti David , Caillol Fabrice , Chaput Ulriikka , Perrot Bastien , Koch Stéphane , Vuitton Lucine , Jacques Jérémie , Valats Jean-Christophe , Poincloux Laurent , Subtil Clément , Chabrun Edouard , Williet Nicolas , Vanbiervliet Geoffroy , Belkhodja Hichem , Charachon Antoine , Wangermez Marc , Coron Emmanuel , Cholet Franck , Privat Jocelyn , Le Baleur Yann , Bichard Philippe , Ah Soune Philippe , Lecleire Stéphane , Palazzo Maxime 
Published: December 2019

Objectives
The results of only a few endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) for pancreatic solid pseudopapillary neoplasm (SPN) have been published, and the safety of the procedure has never been investigated. Our study compared the recurrence rate in patients with and without preoperative EUS-FNA.

Methods
This European multicenter registry-based study was conducted in 22 digestive units, and retrospectively included all patients who underwent complete resection of a pancreatic SPN from 2000 to 2018. Patients with and without initial EUS-FNA were compared, and postsurgery recurrence and the associated risk factors were evaluated.

Results
A complete resection of a pancreatic SPN was performed in 149 patients (133 women, 89%), with a mean age of 34 (standard deviation, 14) years. There were no significant differences between the with (78 patients) and without (71 patients) EUS-FNA groups, except for age and tumor size and location.
Preoperative EUS-FNA allowed pancreatic SPN diagnosis in 63/78 cases (81%). After a mean follow-up of 43 (standard deviation, 36) months, recurrence was noted in 4 patients (2.7%). Preoperative EUS-FNA was not correlated with recurrence, but an older age (P = 0.005) was significant.

Conclusions
Preoperative EUS-FNA does not affect pancreatic SPN recurrence. In this series, old age was significantly correlated with recurrence.
ATM-Mutated Pancreatic Cancer
Authors: Martino Candice , Pandya Deep , Lee Ronald , Levy Gillian , Lo Tammy , Lobo Sandra , Frank Richard C. 
Published: December 2019

Abstract
Metastatic pancreatic cancer (PC) is an aggressive malignancy, with most patients deriving benefit only from first-line chemotherapy. Increasingly, the recommended treatment for those with a germline mutation in a gene involved in homologous recombination repair is with a platinum drug followed by a poly (ADP-ribose) polymerase (poly adenosine phosphate-ribose polymerase [PARP]) inhibitor. Yet, this is based largely on studies of BRCA1/2 or PALB2 mutated PC. We present the case of a 44-year-old woman with ATM-mutated PC who achieved stable disease as the best response to first-line fluorouracil, leucovorin, irinotecan, and oxaliplatin, followed by progression on a PARP inhibitor. In the setting of jaundice, painful hepatomegaly, and a declining performance status, she experienced rapid disease regression with the nonplatinum regimen, gemcitabine plus nab-paclitaxel. Both physical stigmata and abnormal laboratory values resolved, imaging studies showed a reduction in metastases and her performance status returned to normal. Measurement of circulating tumor DNA for KRAS G12R by digital droplet polymerase chain reaction confirmed a deep molecular response. This case highlights that first-line treatment with a platinum-containing regimen followed by PARP inhibition may not be the best choice for individuals with ATM-mutated pancreatic cancer. Additional predictors of treatment response are needed in this setting.
FOXO3a Activation by HDAC Class IIa Inhibition Induces Cell Cycle Arrest in Pancreatic Cancer Cells
Authors: Usami Makoto , Kikuchi Shohei , Takada Kohichi , Ono Michihiro , Sugama Yusuke , Arihara Yohei , Hayasaka Naotaka , Nakamura Hajime , Ikeda Yuuki , Hirakawa Masahiro , Yoshida Makoto , Miyanishi Koji , Kobune Masayoshi , Kato Junji 
Published: December 2019

Objectives
Pancreatic cancer (PC) is highly aggressive with multiple oncogenic mutations. The efficacy of current chemotherapy is poor, and new therapeutic targets are needed. The forkhead box (FOX) proteins are multidirectional transcriptional factors strongly implicated in malignancies. Their expression is consistently suppressed by several oncogenic pathways such as PI3K/AKT signaling activated in PC. A recent study showed that class IIa histone deacetylases (HDAC) can act as a transcriptional suppressor. In this study, we hypothesized that HDAC class IIa inhibition would upregulate FOXO3a expression, thereby inducing its transcription-dependent antitumor effects.

Methods
We confirmed the change of FOXO3a expression and the effect of the cell growth inhibition by HDAC class IIa inhibition in AsPC-1 cells. Because FOXO3a is subject to ubiquitylation-mediated proteasome degradation, we examined the synergistic activation of FOXO3a by HDAC class IIa selective inhibitor TMP269 combined with proteasome inhibitor carfilzomib.

Results
We observed that TMP269 induced FOXO3a expression in a dose-dependent manner and inhibited cell growth in AsPC-1 cells. G1/S arrest was observed. FOXO3a expression was further increased and cell growth inhibition was dramatically enhanced by TMP269 combined with carfilzomib.

Conclusions
Dual inhibition of class IIa HDACs and proteasome could be a promising new strategy for modifying FOXO3a activity against PC.
Nuclear Factor-κB Increases Intracellular Calcium by Upregulation of Na+-Ca2+ Exchanger 1 in Cerulein-Induced Acute Pancreatitis
Authors: Chen Yuyang , Li Wanling , Xie Rui , Tang Bo , Xiao YuFeng , Yang Feng , Chen Siyuan , Yu Senyuan , Yang Shiming , Dong Hui , Guo Hong , Liu Jing-Jing 
Published: December 2019

Objectives
The mechanisms underlying pathogenesis of acute pancreatitis (AP) are still not completely understood. An early, critical feature of AP is aberrant calcium (Ca2+) signaling, termed Ca2+ overload, within pancreatic acinar cells. This study aimed to develop a model system in rats for AP induction to study the contribution of the Na+-Ca2+ exchanger 1 (NCX1) ion channel in AP pathogenesis.

Methods
To establish a rat model of AP induction, cerulein or l-arginine were intraperitoneally injected and tissue was histologically analyzed by hematoxylin and eosin staining. A cell culture-based model for AP induction was similarly created through cerulein treatment of AR42J cells. Induction of AP was also examined following exposure to the NXC1-targeted inhibitor KB-R7943. The expression of each gene was detected by Western blotting, immunofluorescence, immunohistochemistry, or quantitative reverse transcription polymerase chain reaction. Transcriptional regulation by nuclear factor (NF)-κB was detected using an NCX1 promoter-fusion dual luciferase reporter system. Cytosolic Ca2+ was measured using a fluorescent calcium indicator.

Results
We found that cerulein induced NCX1 expression via activation of nuclear factor NF-κB, which potentially binds to the NCX1 promoter to induce its transcription.

Conclusions
Our findings reveal a regulatory pathway through NF-κB/NCX1 governing Ca2+ overload in AP development, thus providing potential targets for AP treatment.
A Modified Technique Using Levator Aponeurosis–Müller Muscle–Reinforced Plication for Blepharoptosis Correction
Authors: Cai Jia-qin , Zhu Quan-chao , Ma Jing-jing , Hu Xian , He Gui-juan , Li Hua 
Published: December 2019

Background
Blepharoptosis is a common and challenging clinical problem for oculoplastic surgeons, and various surgical techniques have been used to correct ptosis. The aims of this study were to present the clinical results of a modified technique using levator aponeurosis–Müller muscle–reinforced plication for blepharoptosis correction and to demonstrate its advantages over conventional advancement or plication methods.

Methods
This study was conducted in the Plastic Surgery Department of Sir Run Run Shaw Hospital between April 2017 and September 2018. By using this modified technique, the levator-Müller complex was reinforced with a plication suture that pierced under and through the levator-Müller complex, and the posterior and lower part of the levator-Müller complex was advanced to the tarsus, which provided permanent, reliable adhesion. The primary outcome was marginal reflex distance 1 preoperatively and postoperatively. Secondary outcomes were the cosmetic outcome, complications, and operative time.

Results
Eighty-six patients (169 eyelids) underwent this modified surgery. Patients' average age was 26 ± 7.6 years, and the median follow-up was 14 months. The preoperative and postoperative mean marginal reflex distance 1 values were 1.72 ± 0.32 and 3.69 ± 0.28 mm, respectively. The amount of plication ranged from 4 to 15 mm. The overall surgical success rate was 88.17%. Some complications were observed including undercorrection (5.92%), asymmetry (4.73%), lagophthalmos (0.59%), and conjunctival prolapse (0.59%).

Conclusions
The modified technique provided good functional and cosmetic outcomes for blepharoptosis correction by avoiding unpredicted adhesion, and it has its advantages including simplicity, easy adjustment of the eyelid height intraoperatively, minimal edema formation, and high success rate.
Management of Sciatic Nerve Defects
Authors: Mathieu Laurent , Addas Bassam M. J. , Irimura Sanae C. , Oberlin Christophe , Belkheyar Zoubir 
Published: December 2019

Abstract
Management of sciatic nerve injuries can be difficult for surgeons without a special interest in nerve surgery as they would only treat a handful of such cases for many years. Sciatic nerve defects pose the greatest repair challenges, with nerve grafting producing mixed results because of the large size of the nerve in both diameter and length. This article first presents the peculiarities of sciatic nerve defects management, based on the authors experience and a literature review. Various issues are dealt with: When to operate depending on the injury mechanism? What are the results of nerve autografting and allografting? On which component should the repair focus in very large defects? Subsequently, alternatives to conventional nerve grafting are proposed. The authors stress the usefulness of direct nerve suture with knee flexion at 90 degrees, which permits bridging of gaps as much as 8 cm in length. For larger defects, other procedures should be considered: long vascularized nerve grafting in complete lesions, short grafting with knee flexed, or tendon transfers in partial lesions.
Evaluation of Open Reduction and Internal Fixation of Mandibular Condyle Fracture by Intraoperative Cone-Beam Computed Tomography in a Hybrid Operating Room
Authors: Sukegawa Shintaro , Masui Masanori , Kanno Takahiro , Miki Masayuki , Nakamoto Hitoshi , Furuki Yoshihiko 
Published: December 2019

Abstract
Condylar fractures are the most common fractures of the mandible, and treatment of mandibular condylar fractures by maxillofacial surgeons is a very important procedure. However, the surgical approaches have anatomical limitations. Therefore, it is difficult to evaluate the reduction achieved in open reduction and internal fixation because of the uncertainty in securing a sufficient operative field. As a potential solution, the authors evaluated the benefits of intraoperative cone-beam computed tomography (CBCT) with high image quality performed in a hybrid operating room. Intraoperative CBCT is easy to perform in a hybrid operating room, and it is possible to quickly evaluate high-quality CT images, including 3D images. Because the state of reduction of mandibular condylar fractures also affects the prognosis of treatment, more precise reduction and fixation should improve prognoses. The use of CBCT in a hybrid operating room also avoids re-operation, and patients benefit from minimum invasive surgery. Intraoperative CBCT is a very useful strategy for evaluation of mandibular condylar fracture surgical treatment.
Reliability and Educational Features of YouTube Videos About Hernia Operations Performed Using Laparoscopic TEP Method
Authors: Kanlioz Murat , Ekici Ugur 
Published: December 2019

Purpose:
The purpose of this study was to assess the reliability, educational features, and popularity of YouTube videos on inguinal hernia operations using the laparoscopic total extraperitoneal (TEP) method on the basis of objective scoring criteria.

Materials and Methods:
We reviewed the first 50 videos on TEP. To assess the reliability and educational features of the videos, we used the DISCERN questionnaire score (DISCERN), Journal of the American Medical Association Benchmark criteria (JAMAS), and Global Quality Scores (GQS). We then used Video Power Index (VPI) to assess the videos’ power on YouTube, and total extraperitoneal scoring (TEPS) to assess their conformity with the medical technique and also the accuracy, quantity, and quality of the information contained therein.

Results:
In the analysis of the source and contents of videos, the VPI, DISCERN, JAMAS, GQS, and TEPS values of all videos were found to be low. A positive correlation—at a level of 0.988—was found to exist between their number of views and VPI values (r: 0.988, P<0.001). Furthermore, we also observed a positive correlation—at a level of 0.811—between the video’s likes and DISCERN scores (r: 0.811, P: 0.004). There was no other statistically significant relationship between other scores and the quantitative features of videos. A positive correlation—at a level of 0.709—was found between the videos’ GQS and TEPS (r: 0.709, P: 0.022). No correlation was found among other scores.

Conclusion:
Depending on the scoring criteria, the lower scores obtained by those videos show that they do not provide a sufficient level of reliability and quality information.
Blood pressure and cognitive function across the eighth decade: a prospective study of the Lothian Birth Cohort of 1936
Authors: Altschul Drew M , Starr John M , Deary Ian J 
Published: December 2019

AbstractObjectivesWe investigated the associations among blood pressure and cognitive functions across the eighth decade, while accounting for anti-hypertensive medication and lifetime stability in cognitive function.DesignProspective cohort study.SettingThis study used data from the Lothian Birth Cohort 1936 (LBC1936) study, which recruited participants living in the Lothian region of Scotland when aged 70 years, most of whom had completed an intelligence test at age 11 years.Participants1091 members of the LBC1936 with assessments of cognitive ability in childhood and older adulthood, and blood pressure measurements in older adulthood.Primary and secondary outcome measuresParticipants were followed up at ages 70, 73, 76, and 79, and latent growth curve models and linear mixed models were used to analyse both cognitive functions and blood pressure as primary outcomes.ResultsBlood pressure followed a quadratic trajectory in the eighth decade: on average blood pressure rose in the first waves and subsequently fell. Intercepts and trajectories were not associated between blood pressure and cognitive functions. Women with higher early-life cognitive function generally had lower blood pressure during the eighth decade. Being prescribed anti-hypertensive medication was associated with lower blood pressure, but not with better cognitive function.ConclusionsOur findings indicate an association between early-life cognitive function and later-life blood pressure, specifically among women. However, we did not find support for the hypothesis that rises in blood pressure and worse cognitive decline are associated with one another in the eighth decade.Strengths and limitations of this study-This study used direct blood pressure measurements to model a continuous blood pressure score, as well as anti-hypertensive medication data, which were used to adjust blood pressure measurements.-This study had comprehensive tests of cognitive ability measured in both childhood and old age which allowed us to investigate whether childhood and old age cognitive ability are distinctly related to blood pressure.-Latent growth curve modelling allowed us to evaluate whether changes in either blood pressure or cognitive functions have downstream associations with one another.-Larger samples and longer follow-up times, both from earlier and later in life, are needed to understand the long-term relationships among blood pressure, anti- hypertensive treatment, and cognitive functions.
Extensive Mammalian Germline Genome Engineering
Authors: Yue Yanan , Kan Yinan , Xu Weihong , Zhao Hong-Ye , Zhou Yixuan , Song Xiaobin , Wu Jiajia , Xiong Juan , Goswami Dharmendra , Yang Meng , Lamriben Lydia , Xu Mengyuan , Zhang Qi , Luo Yu , Guo Jianxiong , Mao Shengyi , Jiao Deling , Nguyen Tien Dat , Li Zhuo , Layer Jacob V. , Li Malin , Paragas Violette , Youd Michele E. , Sun Zhongquan , Ding Yuan , Wang Weilin , Dou Hongwei , Song Lingling , Wang Xueqiong , Le Lei , Fang Xin , George Haydy , Anand Ranjith , Wang Shi Yun , Westlin William F. , Güell Marc , Markmann James , Qin Wenning , Gao Yangbin , Wei Hong-jiang , Church George M. , Yang Luhan 
Published: December 2019

AbstractXenotransplantation, specifically the use of porcine organs for human transplantation, has long been sought after as an alternative for patients suffering from organ failure. However, clinical application of this approach has been impeded by two main hurdles: 1) risk of transmission of porcine endogenous retroviruses (PERVs) and 2) molecular incompatibilities between donor pigs and humans which culminate in rejection of the graft. We previously demonstrated that all 25 copies of the PERV elements in the pig genome could be inactivated and live pigs successfully generated. In this study, we improved the scale of porcine germline editing from targeting a single repetitive locus with CRISPR to engineering 13 different genes using multiple genome engineering methods. we engineered the pig genome at 42 alleles using CRISPR-Cas9 and transposon and produced PERVKO·3KO·9TG pigs which carry PERV inactivation, xeno-antigen KO and 9 effective human transgenes. The engineered pigs exhibit normal physiology, fertility, and germline transmission of the edited alleles.In vitroassays demonstrated that these pigs gain significant resistance to human humoral and cell mediated damage, and coagulation dysregulations, similar to that of allotransplantation. Successful creation of PERVKO·3KO·9TG pigs represents a significant step forward towards safe and effective porcine xenotransplantation, which also represents a synthetic biology accomplishment of engineering novel functions in a living organism.One Sentence SummaryExtensive genome engineering is applied to modify pigs for safe and immune compatible organs for human transplantation
Microscopic and mesoscopic effects of reward uncertainty in monkey fronto-parietal areas
Authors: Taghizadeh Bahareh , Foley Nicholas C. , Karimimehr Saeed , Cohanpour Michael , Semework Mulugeta , Sheth Sameer A. , Lashgari Reza , Gottlieb Jacqueline 
Published: December 2019

AbstractTheories of executive function propose that controlled information processing is costly and is allocated according to the behavioral benefits it brings. Computational theories predict that the benefits of new information depend on prior uncertainty, but the cellular effects of uncertainty on the executive network are incompletely understood. Using simultaneous recordings in monkeys, we reveal several mechanisms by which the fronto-parietal network reacts to uncertainty independently of average reward gains. We show that the variance of expected rewards, independently of the value of the rewards, was represented in single neuron and population spiking activity and local field potential (LFP) oscillations. Moreover, uncertainty asymmetrically affected the coherence between spikes and LFPs, selectively suppressing information transmission from the frontal to the parietal lobe but enhancing transmission from the parietal to the frontal lobe, consistent with Bayesian principles of optimal inference under uncertainty.
Method for identification of heme-binding proteins and quantification of their interactions
Authors: Bozinovic Nina , Noé Rémi , Kanyavuz Alexia , Lecerf Maxime , Dimitrov Jordan D. 
Published: December 2019

AbstractThe standard assay for characterization of interaction of heme with proteins is absorbance spectroscopy. However, this approach demands relatively large quantities of proteins and it is difficult to perform in high-throughput manner. Here, we describe an immunosorbent assay based on the covalentin situconjugation of heme to a pre-coated carrier. Advantage of this assay is that it allows both identification of heme-binding proteins and quantification of their binding avidity, using only minimal amounts of protein (1-10 μg). Importantly, the same approach can be used for covalent linkage of other natural or synthetic compounds and analyzing their interactions with proteins.
Idioblasts as pathways for distributing water absorbed by leaf surfaces to the mesophyll inCapparis odoratissima
Authors: Losada Juan M. , Díaz Miriam , Holbrook N. Michele 
Published: December 2019

ABSTRACTCapparis odoratissimais a tree species native to semi-arid environments of the northern coast of South America where low soil water availability coexists with frequent nighttime fog. Previous work with this species demonstrated thatC. odoratissimais able to use water absorbed through its leaves at night to enhance leaf hydration, photosynthesis, and growth.Here, we combine detailed anatomical evaluations of the leaves ofC. odoratissima,with water and dye uptake experiments in the laboratory. We used immunolocalization of pectin and arabinogalactan protein epitopes to characterize the chemistry of foliar water uptake pathways.The abaxial surfaces ofC. odoratissimaleaves are covered with overlapping, multicellular peltate hairs, while the adaxial surfaces are glabrous but with star-shaped “structures” at regular intervals. Despite these differences in anatomy, both surfaces are able to absorb condensed water, but this ability is most significant on the upper surface. Rates of evaporative water loss from the upper surface, however, are coincident with cuticle conductance. Numerous idioblasts connect the adaxial leaf surface and the adaxial peltate hairs, which contain hygroscopic substances such as arabinogalactan proteins and pectins.The highly specialized anatomy of the leaves ofC odoratissimafulfills the dual function of avoiding excessive water loss due to evaporation, while maintaining the ability to absorb liquid water. Cell-wall related hygroscopic compounds present in the peltate hairs and idioblasts create a network of microchannels that maintain leaf hydration and promote the uptake of aerial water.
Structural features recapitulate collective dynamics of inhibitory networks
Authors: Garg Shivik , Singh Divye 
Published: December 2019

AbstractInhibitory interneurons are ubiquitous through-out the central nervous system (CNS) and play an important role in organizing the excitatory neuronal populations into spatiotemporal patterns. These spatiotemporal patterns are believed to play a vital role in encoding sensory information. The olfactory system is a wellknown example where odor information is encoded in temporally evolving activity of the principal neurons and inhibitory interneurons play an important role in generating these patterns. In this work we study how inhibitory interactions generate such patterns in the con-text of odor encoding by simulating random biophysical models of mitral cells. Using the Newman community clustering algorithm we identify synchronously firing groups of neurons that switch in their activity. Our study presents a new method of inferring the dynamics of inhibitory networks from their structure.