bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Majority of Single Versus Double-Row Rotator Cuff Repair Comparisons Fail to Consider Modern Single-Row Techniques
Authors: Maassen Nicholas H. , Somerson Jeremy S. 
Published: May 2020

Background:There is considerable published research comparing single-row (SR) and double-row (DR) rotator cuff repairs. Conclusions of primary studies as well as multiple meta-analyses have differed. One underexplored factor has been the variability in techniques that are identified as “single-row” or “double-row.” Our goal was to conduct a systematic review of primary research and meta-analyses comparing SR and DR fixation techniques, with attention to the specific technique types compared.Methods:We evaluated meta-analyses and individual studies that directly compared SR and DR repairs. Primary studies from 8 high-quality meta-analyses published prior to 2014, and any Level-III or higher studies published from 2014 to 2019, were included. Specific techniques of repair were identified, and the manuscripts were classified on the basis of the specific type of simple or complex SR or DR repair that was performed. Conclusions of the studies were then assessed according to these classifications.Results:Twenty-two studies met the eligibility criteria; 9 were Level-I studies, 7 were Level-II, and 6 were Level-III. Of the 22 studies, only 3 (14%) of the studies compared a biomechanically superior complex SR technique and a DR repair. The remaining 19 (86%) of the studies utilized biomechanically inferior, simple SR techniques in their comparisons.Conclusions:While there is a large amount of published evidence comparing SR and DR rotator cuff repair techniques, there are few studies assessing how the strongest SR techniques compare with DR techniques. This identifies a deficiency in the current understanding of rotator cuff repair techniques. Future studies specifically designed with these techniques in mind will help to further the understanding of which technique is clinically and financially superior in this continuously changing medical environment.Level of Evidence:TherapeuticLevel III. See Instructions for Authors for a complete description of levels of evidence.
Korelasi dan Sidik Lintas Dua Belas Karakter Vegetatif, Generatif dan Komponen Buah Kelapa Dalam Odeska Lobu [Correlation and Path Analysis of Twelve Vegetative, Generative, and Fruit Component Characters of Odeska Lobu Coconut]
Authors: Miftahorrachman NFN , Tulalo Meity , Tenda Elsje , Kumaunang Jeanette , Matana Yulianus R 
Published: May 2020

<p>The use of quality and certified seeds is an absolute requirement in the construction of the seed garden of coconut plant. Lobu tall coconut is one of the potential coconuts owned by North Sulawesi Province. The research was conducted in Lobu Village, Touluwaan District, Southeast Minahasa Regency, North Sulawesi Province from 2016 to 2018. The purpose of the study was to determine the characters that directly have an impact on the yield using path analysis based on Singh and Chaudary Formula on twelve morphology characters using programme of R i386 3.5.2 <em>package Agricole software</em>. The results of a simple correlation analysis show that there is no relationship between morphological characters and number of fruits, but correlates with the weight of whole fruit. The results of the path analysis showed that only the seed weight character had a direct effect on the weight of whole fruit (r = 0.53) and there were no indirect effects. Seed weight characters can be used as a selection criteria of mother palms to increase coconut productivity of Odeska Lobu coconut.</p><p align="center"><strong>ABSTRAK</strong></p><p>Penggunaan benih berkualitas dan bersertifikat merupakan syarat mutlak dalam pembangunan kebun induk tanaman kelapa. Kelapa Dalam Odeska Lobu merupakan salah satu kelapa potensial yang dimiliki daerah Provinsi Sulawesi Utara. Kegiatan dilakukan di Desa Lobu, Kecamatan Touluwaan, Kabupaten Minahasa Tenggara, Provinsi Sulawesi Utara, mulai tahun 2016 sampai dengan tahun 2018, untuk mengetahui karakter-karakter yang berpengaruh langsung terhadap hasil, dilakukan dengan menggunakan analisis sidik lintas berdasarkan formula Singh dan Chaudary terhadap dua belas karakter vegetatif, generatif, dan komponen buah menggunakan program <em>software </em>R i386 3.5.2 <em>package Agricole</em>. Hasil analisis korelasi sederhana menunjukkan tidak terdapat hubungan antara karakter morfologi dengan karakter hasil jumlah buah, tapi berkorelasi dengan karakter hasil berat buah utuh. Hasil analisis lintas menunjukkan hanya karakter berat biji yang berpengaruh langsung terhadap berat buah utuh (r = 0.53) dan tidak terdapat pengaruh tidak langsung. Karakter berat biji dapat dimanfaatkan sebagai kriteria seleksi pohon tetua sebagai sumber benih untuk meningkatkan produktivitas kelapa Dalam Odeska Lobu. </p>
What are the barriers, facilitators and interventions targeting help-seeking behaviours for common mental health problems in adolescents? A systematic review
Authors: Aguirre Antonia , Silva Ignacio , Billings Jo , Jimenez Magdalena , Rowe Sarah 
Published: May 2020

Abstract

Background: Increasing rates of mental health problems among adolescents are of concern. Teens who are most in need of mental health attention are reluctant to seek help. A better understanding of the help-seeking in this population is needed to overcome this gap. Methods: Five databases were searched to identify the principal barriers, facilitators and interventions targeting help-seeking for common mental health problems in adolescents aged 10-19 years. The search was performed in June 2018 and updated in March 2019. Two independent screening processes were made using the eligibility criteria. Quality assessment of each study was performed and findings summarised using a narrative synthesis. Results: 90 studies meet the inclusion criteria for this review for barrier and facilitators (n=54) and interventions (n=36). Stigma and negative beliefs towards mental health services and professionals were the most cited barriers. Facilitators included previous positive experience with health services and mental health literacy. Most interventions were based on psychoeducation, which focused on general mental health knowledge, suicide and self-harm, stigma and depression. Other types of interventions included the use of multimedia and online tools, peer training and outreach initiatives. Overall, the quality of studies was low to medium and there was no general agreement regarding help-seeking definition and measurements. Conclusion: Most of interventions took place in an educational setting however, it is important to consider adolescents outside the educational system. Encouraging help-seeking should come with the increased availability of mental health support for all adolescents in need, but this is still a major challenge for Child and Adolescent Mental Health Services. There is also a need to develop shared definitions, theoretical frameworks and higher methodological standards in research regarding help-seeking behaviours in adolescents. This will allow more consistency and generalisability of findings, improving the development of help-seeking interventions and ensuring timely access to mental health treatments.
Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides
Authors: Chen Meichun , Wang Jieping , Liu Bo , Zhu Yujing , Xiao Rongfeng , Yang Wenjing , Ge Cibin , Chen Zheng 
Published: May 2020

Abstract

Background:There is an urgent need to discover biological biocontrol agents to control bacterial wilt. This study reports on a new lipopeptide-producing biocontrol strain FJAT-46737 and explores its lipopeptidic compounds, and this study investigates the antagonistic effects of these compounds.Results:Based on a whole genome sequence analysis,the new strain FJAT-46737 was identified asBacillusvelezensis, and seven gene clusters responsible for the synthesis of bioactive secondary metabolites in FJAT-46737 were predicted. The antimicrobial results demonstrated that FJAT-46737 exhibited broad-spectrum antimicrobial activitiesin vitroagainst three bacteria and three fungi. Pot experiments showed that the control efficiencies for tomato bacterial wilt of the whole cultures, the 2-fold diluted supernatants and the crude lipopeptide of FJAT-46737 were 66.2%, 82.0%, and 96.2%, respectively. The above results suggested that one of the antagonistic mechanisms of FJAT-46737 was the secretion of lipopeptides consisting of iturins, fengycins and surfactins. The crude lipopeptides had significant antagonistic activities against several pathogens (includingRalstonia solanacearum,Escherichia coliandFusarium oxysporum) and fengycins were the major antibacterial components of the lipopeptides againstR. solanacearum in vitro. Furthermore, the rich organic nitrogen sources (especially yeast extracts) in the media promoted the production of fengycin and surfactin by FJAT-46737. The secretion of these two lipopeptides was related to temperature fluctuations, with the fengycin content decreasing by 96.6% and the surfactins content increasing by 59.9% from 20oC to 40oC. The optimal temperature for lipopeptide production by FJAT-46737 varied between 20oCand 25oC.Conclusions:TheB.velezensisstrain FJAT-46737 and its secreted lipopeptides could be used as new sources of potential biocontrol agents against several plant pathogens, and especially the bacterial wilt pathogenR. solanacearum.
Conservation of underwater cannonball heritage obtained from a shipwreck in the sea of Batavia, Jakarta, Indonesia
Authors: Riyanto Riyanto , Sahroni Imam , Jazuli Muhammad Malthuf , Musawwa M. Miqdam , Cahyandaru Nahar , Wahyuni Endang Tri 
Published: May 2020

Abstract
The Borobudur Conservation Center in Magelang, Central Java, Indonesia has received underwater cannonballs from a shipwreck in the sea near Batavia, Jakarta, Indonesia. This artifact is a shipment from the Directorate for the Preservation of Cultural Heritage and the Indonesian Ministry of Education and Culture. This study aims to conserve the underwater cannonball before it is stored in a museum. The removal of the protective crust of iron artifacts without the correct and proper method can cause rapid corrosion. To prevent damage, artifacts must be conserved in the right solution. This research aims to stop the process of corrosion and conserve iron objects in aqueous alkali solutions, and the potential corrosion was measured. Conservation was conducted in four stages during this research project. The first stage is the identification of weathering, and the second stage is the analysis and characterization of the corroded surface using a microscope, XRD (X-ray diffraction), XRF (X-ray fluorescence), and SEM (scanning electron microscopy). The third stage is the passivation/deactivation process, which was achieved using sodium hydroxide, soapy water and kaffir lime water. The fourth stage is stabilizing/coating the iron cannonball underwater heritage materials as soon as possible using microcrystalline wax to prevent further corrosion, so this stage should solve the conservation problems associated with the object so that the objects can last for a long time. Dry and wet activated corrosion was characterized by applying XRD to the obtained mineral akageneite. The akageneite minerals were actively corroded and contained high concentrations of Cl atoms revealing dry and wet activated corrosion of 66.603% and 64.963%, respectively. After being conserved with several steps and NaOH, soapy water and kaffir lime water, inactive corrosion was observed. Based on the results of the analysis performed with XRF, the cannonball does not contain Cl, and the Fe content is 98.99%. The conservation method used in this research is excellent and appropriate to conserve cultural heritage materials, including underwater iron cannonballs.
Association between Hemoglobin and Diabetic Peripheral Neuropathy at Different Glycosylated Hemoglobin Levels in Type 2 Diabetes Mellitus Patients
Authors: Zhou Lingling , Xu Xiaojuan , Sheng Hui , Qu Shen , Cui Ran 
Published: May 2020

Abstract
Background: To investigate the possible association of diabetic peripheral neuropathy (DPN) and serum hemoglobin levels in type 2 diabetes mellitus (T2DM) patients with different glycosylated hemoglobin levels.Methods: A total of 995 patients were involved in this retrospective cross-sectional study. Laboratory medical data and electromyography results from the patients were collected. The patients were divided into five groups according to their HbA1c levels (Group 1: HbA1c ≤7%, Group 2: HbA1c 7% to ≤8%, Group 3: HbA1c 8% to ≤9%, Group 4: 9% to ≤10%, and Group 5: >10%). Differences in clinical data in the five groups were compared. Analysis of variance (ANOVA) and binary logistic regression analysis were used. All analyses were performed using SPSS 24 (SPSS Inc., Chicago, IL, USA). P value < 0.05 was considered to indicate statistical significance.Results: A correlation was found between the prevalence of DPN and anemia in both sex groups. Age, BMI, duration of diabetes, ALT, serum creatinine, TC, TG, LDL, FPG, FCP, and hemoglobin A1c differed to a statistically significant extent in different groups. Group 5 showed the highest prevalence of DPN in both sex groups. Hemoglobin levels were higher in Group 5 than in other groups, while composition of anemia was not statistically different. Binary logistic regression showed hemoglobin to be negatively related to the prevalence of DPN in Group 5 in men and in Group 4 in women.Conclusion: Hemoglobin level was negatively associated with the occurrence of DPN at the HbA1c level of >10% in men and 9 to 10% in women in T2DM patients. HbA1c must be considered in exploring the correlation between hemoglobin and DPN.
USP7 regulates melanoma progression through PI3K/Akt/FOXO and AMPK Pathways
Authors: Teichmann Alexander , Jia Jing , Gao Lanyang , Zhu Danli , Wang Qin , Yin Shigang , QIANG Huiyan , Wieland Heinrich , Zhang Jinyue , Bao Zheng 
Published: May 2020

Abstract
Background: Increasing evidence suggests that deubiquitinase USP7 participates in tumor progression by various mechanisms and serves as a potential therapeutic target. However, its functional role in melanoma remains elusive and needs to be investigated. Methods: The down-regulation of USP7 in A375 human melanoma cells was determined by high resolution liquid chromatography mass spectrometry (LC-MS/MS). The effects of USP7 expression on proliferation and apoptosis of melanoma cells were examined by western blot, Immunohistochemical and flow cytometry. Further study knock-down of USP7 in A375 cell lines, especially knockout USP7 using CRISPR-Cas9, verified USP7 regulate cell proliferation in vivo and in vitro .Western blot and qRT-PCR, Immunofluorescence were performed to investigate the mechanisms by which USP7 mediated melanoma growth through regulating the PI3K/Akt/FOXO pathways activity Results: Proteomic and western blotting analysis show that inhibition of USP7 increases expression of PRKAB1, CASP7, and PPP2R3A, attenuates expression of ATP6V0C and PEX11B. Furthermore, ATP6V0C and PEX11B are proposed to be the substrate for USP7 function. Conclusions: Our findings demonstrate that PI3K/Akt/FOXO and AMPK signaling pathways can be regulated by USP7 during melanoma progression and provide USP7 as an attractive anticancer target for melanoma.
Evaluation Of Motor Performance In Cognitively Impaired Elderly Patients With Berg Test: A pilot study
Authors: Sutela Annamaija , Kauppila Timo 
Published: May 2020

Abstract
Objectives We studied whether we can determine a degree of cognitive impairment in which the testing for need of physiotherapy cannot be evaluated without continuous non-verbal instructions in the elderly patients.Present study was a retrospective observational quasi-experimental cohort study performed with patients of geriatric primary care hospital. Fifty-seven aged (>65 years) patients who are aiming to be rehabilitated to home-like facilities participated this study. We compared groups of different levels of cognitive performance (normal cognition, mild, moderate, and severe cognitive impairment) as determined with MiniMental(MMSE)-test by using their motor performance in Berg test.ResultsSeverely cognitively impaired patients (MMSE≤11) were equally able to perform the Berg test as their less cognitively compromised controls if they were continuously advised non-verbally. However, standing without support, sitting without support, reaching forward, watching backwards by turning as well as turning around 360° were too difficult tasks for these patients to be performed without continuous extra assistance given by the physiotherapists. Even the most cognitively impaired patients may be able to perform a motor program in physiotherapy. However, with these patients autonomous exercise is likely to fail and excessive work by physiotherapists is thereby required for their training.
The Prognostic Value of Thyroid Stimulating Immunoglobulin in the Management of Graves’ Disease
Authors: Chen Xinxin , Zhou Yulin , Zhou Mengxi , Qi Yicheng , Wang Weiqing , Ning Guang , Wang Shu 
Published: May 2020

Abstract
Background: The bioassay of thyroid-stimulating immunoglobulin (TSI) appears to be more sensitive than the commonly used TSH binding inhibition assay (TBII), also known as thyroid receptor antibody assay or TRAb.Methods: An observational study was conducted to evaluate the prognostic value of TSI in clinic. Patients with different etiologies of thyrotoxicosis were enrolled to verified the diagnostic value of TSI.Results: 77 Graves’ disease (GD) patients who were treated with anti-thyroid drug (ATD) were in a continuous follow-up until 1 year after ATD discontinuation. Commercial kits of TSI (Thyretain™) and M22-TBII were used and compared. TSI was all negative in healthy controls, Hashimoto thyroiditis, subacute thyroiditis. TSI value was highest in untreated GD patients (P < 0.001). Under ATD treatment, TSI value decreased gradually. 21 patients had positive TSI at the end of treatment. According to clinical fate of patients with GD after withdrawal ATD, TSI value and positivity in patients with relapse were significantly higher than that reported in patients with remission (P = 0.001, P < 0.001). After adjustment for age, gender, initial TRAb, initial TSI, and TRAb at the end of treatment, the odds ratio (OR) of positive TSI for the risk of relapse was 33.271 (95% confidence interval [CI]: 4.741-233.458, P < 0.001) and OR of quantitative TSI was 1.009 (95% CI: 1.002–1.015, P < 0.001).Conclusions: TSI is a good predictor of relapse in patients with GD subjected to ATD treatment. It might be safer to discontinue ATD when TSI and TRAb were both negative.
A Novel Neoagarotriose-Producing Agarase from the Marine Bacterium Gilvimarinus Agarilyticus JEA5
Authors: Lee Youngdeuk , Jo Eunyoung , Lee Yeon-Ju , Choi Hansol , Eom Tae-Yang , Gang Yehui , Kang Yoon-Hyeok , Marasinghe Svini Dileepa , Hettiarachchi Sachithra Amarin , Kang Do-Hyung , Oh Chulhong 
Published: May 2020

Abstract
Background The degradation of agar by bacterial agarases has many commercial and academic applications. We recently identified a novel neoagarotriose-producing β-agarase, Gaa16B, in the marine bacterium Gilvimarinus agarilyticus JEA5. This is the first report to describe neoagarotriose production from β-agarase.Results The Gaa16B agarase, which belongs to the glycoside hydrolase 16 (GH16) family of β-agarases, shows less than 70.9% amino acid similarity with previously characterized agarases. The coding region of Gaa16B is 1800 bp long, encoding 600 amino acids, and exhibits features typical of agarases belonging to the GH16 family. A recombinant Gaa16B lacking the carbohydrate binding region (rGaa16Bc) was overexpressed in Escherichia coli and purified as a maltose-binding protein (MBP) fusion protein. Activity assays revealed the optimal temperature and pH of rGaa16Bc to be 55 °C and pH 6–7, respectively, and the protein was highly stable at 55 °C for 90 min. Additionally, rGaa16Bc activity was strongly enhanced (2.3-fold) in the presence of 2.5 mM MnCl2. The Km and Vmax of rGaa16Bc for agarose were 6.4 mg/ml and 953 U/mg, respectively. Thin layer chromatography analysis revealed that rGaa16Bc can hydrolyze agarose into neoagarotetraose, neoagarotriose, and neoagarobiose, and the production of neoagarotriose by rGaa16Bc was successfully validated by high-resolution electrospray ionization mass spectrometry.Conclusion The biochemical properties of Gaa16B and the results of the hydrolytic pattern analysis suggest that Gaa16B could be useful to produce functional neoagaro-oligosaccharides for industrial applications.
Comparison of Clinical Features and Prognostic Factors of Polymyositis/Dermatomyositis Associated Interstitial Lung Disease According to Autoantibodies: Anti-Aminoacyl tRNA Synthetase Antibodies Versus Anti-Melanoma Differentiation-Associated Gene 5 Antibody.
Authors: Gao Yujuan , Li Yan , Yan Xin , Xie Miaomiao , Li Hui , Huang Mei , Ding Jingjing , Cao Min , Dai Jinghong , Cai Hourong 
Published: May 2020

Abstract
Objective: The serum myositis-specific autoantibodies has been considered to be relatively specific and be useful for diagnosis of polymyositis/dermatomyositis associated interstitial lung disease (PM/DM-ILD). The goal of our retrospective study was to identify clinical features and prognostic factors for PM/DM-ILD based on serological phenotypes.Methods: PM/DM-ILD patients were diagnosed in the Department of Respiratory Medicine, Nanjing Drum Tower Hospital. MSAs were measured by anti-myositis antibody profile IgG detection kit. Based on the results of MSAs, the patients were divided into three groups: anti-MDA5 group, anti-Jo-1 group and other anti-ARS group. Kaplan-Meier, log rank, Kruskal-Wallis test and chi-square tests were used for analysis.Results: We identified 30 patients (22.0%) with positive anti-MDA5, 42 patients (31.0%) with positive anti-Jo-1 and 64 patients(47.0%) with other anti-ARS. acute disease onset was more frequently observed in the anti-MDA5 group (P = 0.005). The highest mortality rate was in the anti-MDA5 group (66.7%, P < 0.001). The overall survival of patients with anti-Jo-1 was significantly better than that of patients with anti-MDA5 (P < 0.001) and similar to that of patients with other anti-ARS. The acute disease onset (P = 0.002), DAD pattern for HRCT imaging (P < 0.001), current smokers (P = 0.02), presence of Anti-MDA5 (P < 0.001), fever (P < 0.001) were significantly associated with the mortality of the study population. Interestingly, in the subgroup survival analysis for anti-MDA5 group, age was a risk factor for death of patients with anti-MDA5 (P = 0.02), and the treatment with PSL pulse and IVIG were markedly correlated with high mortality (P < 0.001 and P = 0.001, respectively).Conclusion: Anti–MDA5 antibody is significantly associated with associated with worse prognosis in PM/DM-ILD patients. The application of PSL pulse and IVIG are not necessarily an effective treatment for positive anti-MDA5 patients.