bluuebunny/crossref_metadata_2025_split huggingface dataset:
The relationship between a microfinance-based healthcare delivery platform, health insurance coverage, health screenings, and disease management in rural Western Kenya
Authors: Rosenberg Molly , Amisi James Akiruga , Szkwarko Daria , Tran Dan N. , Genberg Becky , Luetke Maya , Kianersi Sina , Namae Jane , Laktabai Jeremiah , Pastakia Sonak 
Published: September 2020

Abstract
Background
Structural barriers often prevent rural Kenyans from receiving healthcare and diagnostic testing. The Bridging Income Generation through grouP Integrated Care (BIGPIC) Family intervention facilitates microfinance groups, provides health screenings and treatment, and delivers education about health insurance coverage to address some of these barriers. This study evaluated the association between participation in BIGPIC microfinance groups and health screening/disease management outcomes.

Methods
From November 2018 to March 2019, we interviewed a sample of 300 members of two rural communities in Western Kenya, 100 of whom were BIGPIC microfinance members. We queried participants about their experiences with health screening and disease management for HIV, diabetes, hypertension, tuberculosis, and cervical cancer. We used log-binomial regression models to estimate the association between microfinance membership and each health outcome, adjusting for key covariates.

Results
Microfinance members were more likely to be screened for most of the health conditions we queried, including those provided by BIGPIC [e.g. diabetes: aPR (95% CI): 3.46 (2.60, 4.60)] and those not provided [e.g. cervical cancer: aPR (95% CI): 2.43 (1.21, 4.86)]. Microfinance membership was not significantly associated with health insurance uptake and disease management outcomes.

Conclusions
In rural Kenya, a microfinance program integrated with healthcare delivery may be effective at increasing health screening. Interventions designed to thoughtfully and sustainably address structural barriers to healthcare will be critical to improving the health of those living in low-resource settings.
Growth faltering in early infancy: highlights from a two-day scientific consultation
Authors: Aneja Satinder , Kumar Praveen , Choudhary Tarun Shankar , Srivastava Akanksha , Chowdhury Ranadip , Taneja Sunita , Bhandari Nita , Daniel Abner , Menon Purnima , Chellani Harish , Bahl Rajiv , Bhan Maharaj Kishan 
Published: September 2020

AbstractBackgroundFaltering of growth in early life has been recognized as a public health challenge among Indian babies. A two-day consultation on growth faltering in early infancy was organized to examine the data and evidence on identification and management of early growth failure and to identify knowledge gaps and future areas of research. The consultation was supported by the Biotechnology Industry Research Assistance Council (BIRAC), the Indian Academy of Pediatrics (Nutrition Chapter), Vardhman Mahavir Medical College and Safdarjung Hospital, and the Society for Applied Studies. It brought together researchers, clinicians, policy makers and program managers.
The accuracy of four commercial broth microdilution tests in the determination of the minimum inhibitory concentration of colistin
Authors: Yusuf Erlangga , van Westreenen Mireille , Goessens Wil , Croughs Peter 
Published: September 2020

AbstractColistin is considered as one of the last-resort antibiotics and reliable antimicrobial susceptibility testing is therefore crucial. The reference standard for AST according to EUCAST and CLSI is broth microdilution (BMD). However, BMD is labor intensive to perform. Commercial antimicrobial susceptibility tests derived from BMD method are available. We investigated the performance of four different commercial tests: Sensititre™, SensiTest™ Colistin, Micronaut MIC Strip Colistin and UMIC Colistin using 70 clinical isolates (half of them was deemed by VITEK2 as resistant), including isolates from cystic fibrosis patients and mcr-1 bearing isolates. We used two reference standards: BMD and composite MIC as determined by all four tests. Sensititre™ had essential agreement (EA, defined as minimum inhibitory concentration within ± 1 dilution) of 87% and 89% compared to BMD and composite reference standard, respectively. For SensiTest™, the EA’s were 93% and 90%. For UMIC, 87% and 90%, and for Micronaut, 83% and 84%. All four tests demonstrated categorical agreement (CA) above 90%. CA for SensiTest™ and Micronaut was both 96%, UMIC 94%, and Sensititre™ 93%. All tests were reproducible as tested in two quality control isolates. In conclusion, in clinical isolates from a large referral center, the four commercial tests for determination of colistin minimum inhibitory concentrations showed acceptable performance.
Molecular basis of carrageenan-induced cytokines production in macrophages
Authors: Lopes Alexandre H. , Silva Rangel L. , Fonseca Miriam D. , Gomes Francisco I. , Maganin Alexandre G. , Ribeiro Lucas S. , Marques Lucas Maciel Mauriz , Cunha Fernando Q. , Alves-Filho Jose C. , Zamboni Dario S. , Lopes Norberto P. , Franklin Bernardo S. , Gombault Aurélie , Ramalho Fernando Silva , Quesniaux Valerie F. J. , Couillin Isabelle , Ryffel Bernhard , Cunha Thiago M. 
Published: September 2020

Abstract
Background
Low molecular weight carrageenan (Cg) is a seaweed-derived sulfated polysaccharide widely used as inflammatory stimulus in preclinical studies. However, the molecular mechanisms of Cg-induced inflammation are not fully elucidated. The present study aimed to investigate the molecular basis involved in Cg-induced macrophages activation and cytokines production.

Methods
Primary culture of mouse peritoneal macrophages were stimulated with Kappa Cg. The supernatant and cell lysate were used for ELISA, western blotting, immunofluorescence. Cg-induced mouse colitis was also developed.

Results
Here we show that Cg activates peritoneal macrophages to produce pro-inflammatory cytokines such as TNF and IL-1β. While Cg-induced TNF production/secretion depends on TLR4/MyD88 signaling, the production of pro-IL-1β relies on TLR4/TRIF/SYK/reactive oxygen species (ROS) signaling pathway. The maturation of pro-IL1β into IL-1β is dependent on canonical NLRP3 inflammasome activation via Pannexin-1/P2X7/K+ efflux signaling. In vivo, Cg-induced colitis was reduced in mice in the absence of NLRP3 inflammasome components.

Conclusions
In conclusion, we unravel a critical role of the NLRP3 inflammasome in Cg-induced pro-inflammatory cytokines production and colitis, which is an important discovery on the pro-inflammatory properties of this sulfated polysaccharide for pre-clinical studies.

Graphical Abstract
Carrageenan (Cg) is one the most used flogistic stimulus in preclinical studies. Nevertheless, the molecular basis of Cg-induced inflammation is not totally elucidated. Herein, Lopes et al. unraveled the molecular basis for Cg-induced macrophages production of biological active IL-1β. The Cg-stimulated macrophages produces pro-IL-1β depends on TLR4/TRIF/Syk/ROS, whereas its processing into mature IL-1β is dependent on the canonical NLRP3 inflammasome.
Factor VIII inhibitor development in Egyptian hemophilia patients: does intron 22 inversion mutation play a role?
Authors: Sherief Laila M. , Gaber Osama A. , Youssef Hala Mosaad , Sherbiny Hanan S. , Mokhtar Wesam a , Ali Asmaa A. A. , Kamal Naglaa M. , Abdel Maksoud Yehia H. 
Published: September 2020

AbstractBackgroundHemophilia A (HA) is an X-linked recessive bleeding disorder characterized by qualitative and quantitative deficiency of factor VIII (FVIII). The development of inhibitor antibodies against FVIII is the most challenging complication of treatment. Mutations in the FVIII gene is one of the genetic factors that leads to development of FVIII inhibitors especially intron 22 inversion (Inv22).ObjectivesThis study was carried out to assess the frequency of Inv22 of FVIII gene in Egyptian patients with hemophilia A and its role as a risk factor for developing inhibitors.Patients and methodsSeventy-two patients with severe HA and 48 patients with moderate HA were enrolled in the current study. All patients were treated on demand with either plasma-derived factor VIII or recombinant factor VIII concentrates. Genotyping of FVIII Inv22 was performed by LD-PCR while the presence and magnitude of inhibitor activity in blood was determined by the Bethesda assay.ResultsAround 23% of all hemophilia cases had positive Inv22. Intron 22 inversion mutation was detected in 6 and 33% of patients with moderate and severe HA respectively. Twenty-one cases (18%) of all hemophilic patients developed inhibitors. Thirty-7% of patients with Inv22 had inhibitor in their blood, almost all, but one, had severe HA. The risk of an inhibitor development during replacement therapy was four folds higher among Inv22 positive cases as compared with mutation negative peers (OR 4.3, 95% CI 1.6–11.9,P = 0.003).ConclusionsThe prevalence of Inv22 of F VIII in Egyptian hemophiliacs is nearly like that of other population. This mutation was more frequently detected among severe hemophilic patients as compared with moderately affected peers. The presence of Inv22 mutation significantly predispose to FVIII inhibitor development.
Enterotropism of highly pathogenic avian influenza virus H5N8 from the 2016/2017 epidemic in some wild bird species
Authors: Caliendo Valentina , Leijten Lonneke , Begeman Lineke , Poen Marjolein J. , Fouchier Ron A. M. , Beerens Nancy , Kuiken Thijs 
Published: September 2020

AbstractIn 2016/2017, H5N8 highly pathogenic avian influenza (HPAI) virus of the Goose/Guangdong lineage spread from Asia to Europe, causing the biggest and most widespread HPAI epidemic on record in wild and domestic birds in Europe. We hypothesized that the wide dissemination of the 2016 H5N8 virus resulted at least partly from a change in tissue tropism from the respiratory tract, as in older HPAIV viruses, to the intestinal tract, as in low pathogenic avian influenza (LPAI) viruses, allowing more efficient faecal-oral transmission. Therefore, we determined the tissue tropism and associated lesions in wild birds found dead during the 2016 H5N8 epidemic, as well as the pattern of attachment of 2016 H5N8 virus to respiratory and intestinal tissues of four key wild duck species. We found that, out of 39 H5N8-infected wild birds of 12 species, four species expressed virus antigen in both respiratory and intestinal epithelium, one species only in respiratory epithelium, and one species only in intestinal epithelium. Virus antigen expression was association with inflammation and necrosis in multiple tissues. The level of attachment to wild duck intestinal epithelia of 2016 H5N8 virus was comparable to that of LPAI H4N5 virus, and higher than that of 2005 H5N1 virus for two of the four duck species and chicken tested. Overall, these results indicate that 2016 H5N8 may have acquired a similar enterotropism to LPAI viruses, without having lost the respirotropism of older HPAI viruses of the Goose/Guangdong lineage. The increased enterotropism of 2016 H5N8 implies that this virus had an increased chance to persist long term in the wild waterbird reservoir.
Stego key recovery method for F5 steganography with matrix encoding
Authors: Liu Jiufen , Yang Chunfang , Wang Junchao , Shi Yanan 
Published: September 2020

AbstractWhen embedding secret message into image by steganography with matrix encoding, there are still no effective methods to recover the stego key because it is difficult to statistically distinguish the stego coefficient sequences selected by true and false keys. Therefore, this paper proposes a method for recovering the stego key of a typical JPEG (Joint Photographic Experts Group) image steganography—F5 which composes of the check matrix and shuffling key. Firstly, the check matrix is recovered based on the embedding ratio estimated by quantitative steganalysis. The shuffling key is then recovered based on the distribution difference between the bit sequences extracted by the true and false shuffling keys. Additionally, the cardinality of the shuffling key space is significantly reduced by examining the extracted encoding parameter and message length. Experimental results show that the proposed method can recover the stego key accurately and efficiently, even when the existing Xu’s method fails for the high or very low embedding ratio.
Motor performance and back pain in children and adolescents: a systematic review and meta-analysis protocol
Authors: Noll Matias , Wedderkopp Niels , Mendonça Carolina Rodrigues , Kjaer Per 
Published: September 2020

AbstractBackgroundThe relationship between motor performance and back pain in children and adolescents remains unclear. This article describes the protocol for a systematic review to summarize the knowledge about the association between motor performance, such as agility, flexibility, balance, strength, muscle endurance, and cardiorespiratory fitness, and back pain. Thus, our aim is to identify the influence of motor performance on back pain among children and adolescents.MethodsTwo independent researchers will search MEDLINE, Scopus, Embase, SPORTDiscus, and CINAHL databases, with no period or language restrictions. We will include cross-sectional, cohort, case-control, and controlled clinical trial studies based on the following criteria: (a) participants from 6 to 19 years of age, (b) assessment of motor performance, (c) assessment of back pain, and (d) report measures of associations between motor performance and back pain. Study quality and risk of bias will be assessed using an adapted version of the Downs and Black instrument. Grading of Recommendations, Assessment, Development, and Evaluations will be used to assess the strength of the body of evidence. Meta-analyses of association measures will be performed for each type of motor performance, separately for different study types. The results will be reported using forest to show the pooled effect of findings and funnel plots to assess precision of the data. If studies are not homogeneous, results from the meta-analyses will not be reported. Associations will then be synthesized descriptively using a pragmatic approach.DiscussionThis systematic review will provide critical insights into the association between motor performance and back pain among children and adolescents; this information may help support clinical practice guidelines as well as public health programs.Ethics and disseminationProtocol was written according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA).Systematic review registrationPROSPEROCRD42020178496
IoT-enabled directed acyclic graph in spark cluster
Authors: Koo Jahwan , Faseeh Qureshi Nawab Muhammad , Siddiqui Isma Farah , Abbas Asad , Bashir Ali Kashif 
Published: September 2020

AbstractReal-time data streaming fetches live sensory segments of the dataset in the heterogeneous distributed computing environment. This process assembles data chunks at a rapid encapsulation rate through a streaming technique that bundles sensor segments into multiple micro-batches and extracts into a repository, respectively. Recently, the acquisition process is enhanced with an additional feature of exchanging IoT devices’ dataset comprised of two components: (i) sensory data and (ii) metadata. The body of sensory data includes record information, and the metadata part consists of logs, heterogeneous events, and routing path tables to transmit micro-batch streams into the repository. Real-time acquisition procedure uses the Directed Acyclic Graph (DAG) to extract live query outcomes from in-place micro-batches through MapReduce stages and returns a result set. However, few bottlenecks affect the performance during the execution process, such as (i) homogeneous micro-batches formation only, (ii) complexity of dataset diversification, (iii) heterogeneous data tuples processing, and (iv) linear DAG workflow only. As a result, it produces huge processing latency and the additional cost of extracting event-enabled IoT datasets. Thus, the Spark cluster that processes Resilient Distributed Dataset (RDD) in a fast-pace using Random access memory (RAM) defies expected robustness in processing IoT streams in the distributed computing environment. This paper presents an IoT-enabled Directed Acyclic Graph (I-DAG) technique that labels micro-batches at the stage of building a stream event and arranges stream elements with event labels. In the next step, heterogeneous stream events are processed through the I-DAG workflow, which has non-linear DAG operation for extracting queries’ results in a Spark cluster. The performance evaluation shows that I-DAG resolves homogeneous IoT-enabled stream event issues and provides an effective stream event heterogeneous solution for IoT-enabled datasets in spark clusters.
Co-expression of C9orf72 related dipeptide-repeats over 1000 repeat units reveals age- and combination-specific phenotypic profiles in Drosophila
Authors: West Ryan J. H. , Sharpe Joanne L. , Voelzmann André , Munro Anna L. , Hahn Ines , Baines Richard A. , Pickering-Brown Stuart 
Published: September 2020

AbstractA large intronic hexanucleotide repeat expansion (GGGGCC) within the C9orf72 (C9orf72-SMCR8 Complex Subunit) locus is the most prevalent genetic cause of both Frontotemporal Dementia (FTD) and Motor Neuron Disease (MND). In patients this expansion is typically hundreds to thousands of repeat units in length. Repeat associated non-AUG translation of the expansion leads to the formation of toxic, pathological Dipeptide-Repeat Proteins (DPRs). To date there remains a lack of in vivo models expressing C9orf72 related DPRs with a repeat length of more than a few hundred repeats. As such our understanding of how physiologically relevant repeat length DPRs effect the nervous system in an ageing in vivo system remains limited. In this study we generated Drosophila models expressing DPRs over 1000 repeat units in length, a known pathological length in humans. Using these models, we demonstrate each DPR exhibits a unique, age-dependent, phenotypic and pathological profile. Furthermore, we show co-expression of specific DPR combinations leads to distinct, age-dependent, phenotypes not observed through expression of single DPRs. We propose these models represent a unique, in vivo, tool for dissecting the molecular mechanisms implicated in disease pathology, opening up new avenues in the study of both MND and FTD.
Error propagation analysis of seven partial volume correction algorithms for [18F]THK-5351 brain PET imaging
Authors: Oyama Senri , Hosoi Ayumu , Ibaraki Masanobu , McGinnity Colm J. , Matsubara Keisuke , Watanuki Shoichi , Watabe Hiroshi , Tashiro Manabu , Shidahara Miho 
Published: September 2020

Abstract
Background
Novel partial volume correction (PVC) algorithms have been validated by assuming ideal conditions of image processing; however, in real clinical PET studies, the input datasets include error sources which cause error propagation to the corrected outcome.

Methods
We aimed to evaluate error propagations of seven PVCs algorithms for brain PET imaging with [18F]THK-5351 and to discuss the reliability of those algorithms for clinical applications. In order to mimic brain PET imaging of [18F]THK-5351, pseudo-observed SUVR images for one healthy adult and one adult with Alzheimer’s disease were simulated from individual PET and MR images. The partial volume effect of pseudo-observed PET images were corrected by using Müller-Gärtner (MG), the geometric transfer matrix (GTM), Labbé (LABBE), regional voxel-based (RBV), iterative Yang (IY), structural functional synergy for resolution recovery (SFS-RR), and modified SFS-RR algorithms with incorporation of error sources in the datasets for PVC processing. Assumed error sources were mismatched FWHM, inaccurate image-registration, and incorrectly segmented anatomical volume. The degree of error propagations in ROI values was evaluated by percent differences (%diff) of PV-corrected SUVR against true SUVR.

Results
Uncorrected SUVRs were underestimated against true SUVRs (− 15.7 and − 53.7% in hippocampus for HC and AD conditions), and application of each PVC algorithm reduced the %diff. Larger FWHM mismatch led to larger %diff of PVC-SUVRs against true SUVRs for all algorithms. Inaccurate image registration showed systematic propagation for most algorithms except for SFS-RR and modified SFS-RR. Incorrect segmentation of the anatomical volume only resulted in error propagations in limited local regions.

Conclusions
We demonstrated error propagation by numerical simulation of THK-PET imaging.
Error propagations of 7 PVC algorithms for brain PET imaging with [18F]THK-5351 were significant. Robust algorithms for clinical applications must be carefully selected according to the study design of clinical PET data.
Specific airway management for SVC replacement during giant anterior mediastinal tumor resection
Authors: Kawagoe Izumi , Satoh Daizoh , Mitaka Chieko , Fukuda Masataka , Kochiyama Tsukasa , Hayashida Masakazu 
Published: September 2020

Abstract
Background
Giant anterior mediastinal tumor (GAMT) resection is a challenging procedure, for which anesthesiologist might take to need special precautions.

Case presentation
A 48-year-old male patient had been scheduled to undergo GAMT resection and superior vena cava (SVC) replacement. The tumor spread surrounding SVC and left main bronchus (LMB), resulting in small volume of his left lung. A soft left-sided double lumen tube (DLT) was selected to keep the patency of LMB during left one lung ventilation (OLV) against the tumor weight. Semi-awake intubation with spontaneous breathing was selected for DLT insertion to avoid lower airway occlusion. During left OLV after right open thoracotomy, his SPO2 decreased below to 90%. We performed selective right upper lobe bronchial blockade using the combination of DLT and bronchial blocker. The surgery was successfully completed with this strategy.

Conclusions
Although such cases are rare, they are informative for anesthesiologists, providing optional strategies.
Genetic diversity of avocado from the southern highlands of Tanzania as revealed by microsatellite markers
Authors: Juma Ibrahim , Geleta Mulatu , Nyomora Agnes , Saripella Ganapathi Varma , Hovmalm Helena Persson , Carlsson Anders S. , Fatih Moneim , Ortiz Rodomiro 
Published: September 2020

Abstract
Background
Avocado is an important cash crop in Tanzania, however its genetic diversity is not thoroughly investigated. This study was undertaken to explore the genetic diversity of avocado in the southern highlands using microsatellite markers. A total of 226 local avocado trees originating from seeds were sampled in eight districts of the Mbeya, Njombe and Songwe regions. Each district was considered as a population. The diversity at 10 microsatellite loci was investigated.

Results
A total of 167 alleles were detected across the 10 loci with an average of 16.7 ± 1.3 alleles per locus. The average expected and observed heterozygosity were 0.84 ± 0.02 and 0.65 ± 0.04, respectively. All but two loci showed a significant deviation from the Hardy-Weinberg principle. Analysis of molecular variance showed that about 6% of the variation was partitioned among the eight geographic populations. Population FST pairwise comparisons revealed lack of genetic differentiation for the seven of 28 population pairs tested. The principal components analysis (PCA) and hierarchical cluster analysis showed a mixing of avocado trees from different districts. The model-based STRUCTURE subdivided the trees samples into four major genetic clusters.

Conclusion
High diversity detected in the analysed avocado germplasm implies that this germplasm is a potentially valuable source of variable alleles that might be harnessed for genetic improvement of this crop in Tanzania. The mixing of avocado trees from different districts observed in the PCA and dendrogram points to strong gene flow among the avocado populations, which led to population admixture revealed in the STRUCTURE analysis. However, there is still significant differentiation among the tree populations from different districts that can be utilized in the avocado breeding program.