bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long Non-Coding RNA LINC00518 Induces Radioresistance by Regulating Glycolysis Through An miR-33a-3p/HIF-1α Negative Feedback Loop in Melanoma
Authors: Cao Ke , yan Liu , Dong He , Mengqin Xiao , Liang Xiang , Yuxing Zhu 
Published: July 2020

Abstract
Background: The long non-coding RNA (lncRNA),LINC00518, is highly expressed in many human cancers and is involved in cancer progression. However, the potential function and regulatory mechanism of LINC00518 in cutaneous malignant melanoma (CMM) remain unclear. Methods:Short hairpin RNA (shRNA) was used to silence LINC00518 and HIF-1α, and real-time PCR was performed to determine mRNA expression. Then, cell proliferation, colony formation, flow cytometric, scratch, and transwell assays were to examine the influence of LINC00518 silencing on cellular radiosensitivity. Dual luciferase reporter system,CHIP and COIP was used to verify the target relationship between LINC00518,miR‐33a-5b and HIF-1α,.Glycolysis assays were conducted to exam cell glycolysis process. Western blotting was performed to explore the expression of HIF-1α and LDHA. Finally, animal experiments were performed to demonstrate the effect of LINC00518 silencing on the radiosensitivity of melanoma in vivo.Results: LINC00518 expression was significantly upregulated in CMM samples, and LINC00518 levels were associated with poor prognosis of patients with CMM. Knockdown of LINC00518 in CMM cells significantly inhibited cell invasion, migration, proliferation, and clonogenicity. LINC00518-mediated invasion, migration, proliferation, and clonogenicity were negatively regulated by the microRNA, miR-33a-3p, in vitro, which intensified sensitivity to radiotherapy via inhibition of the hypoxia-induced factor 1α (HIF-1α)/lactate dehydrogenase A (LDHA)-glycolysis axis. Additionally, HIF-1α recognized the miR-33a-3p promoter region and recruited histone deacetylase2 (HDAC2), which decreased the expression of miR-33a-3p and formed an LINC00518/miR-33a-3p/HIF-1α negative feedback loop. Furthermore, signalling initially activated glycolysis and radioresistance in CMM cells was recovered by Santacruzamate A (a histone deacetylase inhibitor) and 2-deoxy-D-glucose (a glycolytic inhibitor). Lastly, knockdown of LINC00518 expression sensitized CMM cancer cells to radiotherapy in an in vivo subcutaneously implanted tumour model. Conclusion: LINC00518 was confirmed to be an oncogene in CMM, which induces radioresistance by regulating glycolysis through an miR-33a-3p/HIF-1α negative feedback loop. Our research may provide a potential strategy to improve the treatment outcome of radiotherapy in CMM.
Two Stepped Majority Voting for Efficient EEG Based Emotion Classification
Authors: Ismael Aras Masood , Alçin Ömer F. , Abdalla Karmand Hussein , Şengür Abdulkadir K 
Published: July 2020

Abstract
In this paper, a novel approach that is based on two-stepped majority voting is proposed for efficient EEG based emotion classification. Emotion recognition is important for human-machine interactions. Facial-features and body-gestures based approaches have been generally proposed for emotion recognition. Recently, EEG based approaches become more popular in emotion recognition. In the proposed approach, the raw EEG signals are initially low-pass filtered for noise removal and band-pass filters are used for rhythms extraction. For each rhythm, the best performed EEG channels are determined based on wavelet-based entropy features and fractal dimension based features. The k-nearest neighbor (KNN) classifier is used in classification. The best five EEG channels are used in majority voting for getting the final predictions for each EEG rhythm. In the second majority voting step, the predictions from all rhythms are used to get a final prediction. The DEAP dataset is used in experiments and classification accuracy, sensitivity and specificity are used for performance evaluation metrics. The experiments are carried out to classify the emotions into two binary classes such as high valence (HV) vs low valence (LV) and high arousal (HA) vs low arousal (LA). The experiments show that 86.3% HV vs LV discrimination accuracy and 85.0% HA vs LA discrimination accuracy is obtained. The obtained results are also compared with some of the existing methods. The comparisons show that the proposed method has potential in the use of EEG based emotion classification.
Screening of independent prognostic long non-coding RNA for gastric cancer in TCGA
Authors: Zhang Qiang , Chen Qiongyun , Lv Yinyin , Dong Xuan , Huang Xiaoqing , Yan Changsheng , Xu Hongzhi 
Published: July 2020

Abstract
Background

The global incidence of gastric cancer (GC) ranks the fourth among cancers and its 5-year survival is less than 25%. LncRNAs are vital regulators involved in pathological processes of cancer. It is urgent to screen the prognostic lncRNA in GC.

Method

Expression file and clinical data of GC were downloaded from TCGA. Differentially expressed lncRNAs were calculated by edger R package, followed by the prognosis analysis. COX analysis was conducted to compute the independent factor of GC. Potential signaling pathways that the screened lncRNAs enriched in were evaluated by gene set enrichment analysis (GSEA). At last, Pearson analysis was conducted to predict the possible mechanism of lncRNA in GC process.

Result

ENSG00000224363 was an unfavorable prognostic factor to OS (overall survival) and DFS (disease-free survival) of GC as COX regression analyzed. GSEA analysis indicated that ENSG00000224363 may regulate cell cycle, apoptosis and autophagy of GC cells.

Conclusion

LncRNA ENSG00000224363 is overexpressed in GC, serving as an independent unfavorable prognostic factor.
Effects of Transcranial Direct Current Stimulation (tDCS) on Posture, Movement Planning, and Execution During Standing Voluntary Reach Following Stroke
Authors: Yang Chieh-ling , Gad Alon , Creath Robert A. , Magder Laurence , Rogers Mark W. , Waller Sandy McCombe 
Published: July 2020

Abstract
Background: Impaired movement preparation of both anticipatory postural adjustments and goal directed movement as shown by a marked reduction in the incidence of StartReact responses during a standing reaching task was reported in individuals with stroke. We tested how transcranial direct current stimulation (tDCS) applied over the region of premotor areas (PMAs) and primary motor area (M1) affect movement planning and preparation of a standing reaching task in individuals with stroke. Methods: Each subject performed two sessions of tDCS over the lesioned hemisphere on two different days: cathodal tDCS over PMAs and anodal tDCS over M1. Movement planning and preparation of anticipatory postural adjustment-reach sequence was examined by startReact responses elicited by a loud acoustic stimulus of 123 dB. Kinetic, kinematic, and electromyography data were recorded to characterize anticipatory postural adjustment-reach movement response. Results: Anodal tDCS over M1 led to significant increase of startReact responses incidence at loud acoustic stimulus time point – 500 ms. Increased trunk involvement during movement execution was found after anodal M1 stimulation compared to PMAs stimulation. Conclusions: The findings provide novel evidence that impairments in movement planning and preparation as measured by startReact responses for a standing reaching task can be mitigated in individuals with stroke by the application of anodal tDCS over lesioned M1 but not cathodal tDCS over PMAs. This is the first study to show that stroke-related deficits in movement planning and preparation can be improved by application of anodal tDCS over lesioned M1.Trial registration: ClinicalTrial.gov, NCT04308629, Registered 16 March 2020 - Retrospectively registered, https://www.clinicaltrials.gov/ct2/show/NCT04308629.
Investigating knowledge, attitudes, and practices related to COVID-19 outbreak among Bangladeshi young adults: A web-based cross-sectional analysis
Authors: Banik Rajon , Rahman Mahmudur , Sikder Tajuddin , Rahman Quazi Maksudur , Pranta Mamun Ur Rashid 
Published: July 2020

Abstract
Aim: This study aimed to assess knowledge, attitudes, and practices (KAP) towards COVID-19 among young adults in Bangladesh. Subject and Methods: A cross-sectional survey was conducted from 5th May to 25th May 2020. Young adults aged between 18-35 years were approached via social media to complete an online questionnaire consisted of socio-demographic characteristics and KAP towards COVID-19. Descriptive statistics, t-tests, one-way analysis of variance (ANOVA), and multiple logistic regressions were conducted. Results: Of the 707 survey participants, the majority were male (57.1%), with a mean age of 25.03 (SD: 4.26) years, 60.3% were students, 57% had a bachelor's degree, and 64.4% urban residents. Participants gather information on COVID-19 mostly through Social media (70.4%). Overall, 61.2% had adequate knowledge with 78.9% having a positive attitude towards COVID-19 and only 51.6% had good practices. Most (86.8%) of the participants were confident that COVID-19 will be successfully controlled and Bangladesh was handling the COVID-19 health crisis well (84.20%). Only 75.2% of participants always washed hands with soap or hand-sanitizer and 70.6% wore a mask when going outside the home in recent days. Factors associated with adequate knowledge were being female, having a master's degree and above, and living in an urban area (𝑝 < 0.05). Participants having adequate knowledge of COVID-19 had higher likelihood of positive attitudes (OR: 6.41, 95% CI = 2.34-25.56, 𝑝 = 0.000) and good practices (OR: 8.93, 95% CI = 3.92-38.42, 𝑝 = 0.000). Conclusion: Introducing effective COVID-19 Health campaigns incorporating factors to influence KAP is urgently needed.
Profiling B cell immune responses to identify neutralizing antibodies from convalescent COVID-19 patients
Authors: Huang Lisu , Shen Bingqing , Guo Yu , Shen Shu , Huang Heyu , Xie Qiuling , Chen Mingjie , Zeng Xinxin , Wang Shanshan , Huang Dakun , Huang Mengshi , Qiu Yushu , Han Xiaochen , Jing Qingqing , Lu Minmin , Pi Chunjiang , Wu Jia , Feng Minfang , Li Ouyang , Lu Yun-Yueh , Zhou He , Cui Cheryl , He Jeff , Pan Shuming , Chen Da , Zhou Xingdong , Wang Yuan , Tian Yujie , Wang Wei , Zhang Wanju , Deng Fei , Schweizer Liang , Zhang Hongkai , Sun Kun 
Published: July 2020

Abstract
The pandemic Coronavirus Disease 2019 (COVID-19) causes noticeable morbidity and mortality worldwide. In addition to vaccine and antiviral drug therapy, the use of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) neutralizing antibodies for treatment purposes is a viable alternative. In this study, we aimed to profile the humoral responses and identify neutralizing antibodies against SARS-CoV-2 using high-throughput single-cell sequencing that tailored to B cell receptor sequencing. From two convalescent patients with high serum titer against SARS-COV-2, we identified seven antibodies specifically binding to SARS-CoV-2. Among these, the most potent antibody, P4A1 was demonstrated to block the binding of spike protein to its receptor angiotensin-converting enzyme 2 (ACE2), and prevent the viral infection in neutralization assays with pseudovirus as well as live virus at nM to sub-nM range. Moreover, antibody P4A1 can also bind strongly to spike protein with N354D/D364Y, R408I, W436R, V367F or D614G mutations respectively, suggesting that the antibody alone or in combination with other antibodies that recognize different variations of SARS-CoV-2, may provide a broad spectrum therapeutic option for COVID-19 patients.
Quantitative longitudinal predictions of Alzheimer's disease by multi-modal predictive learning
Authors: Prakash Mithilesh , Abdelaziz Mahmoud , Zhang Linda , Strange Bryan A , Tohka Jussi 
Published: July 2020

Abstract
Background: Quantitatively predicting the progression of Alzheimer’s disease (AD) in an individual on a continuous scale, such as AD assessment scale-cognitive (ADAS-cog) scores, is informative for a personalized approach as opposed to qualitatively classifying the individual into a broad disease category. We hypothesize that multi-modal data and predictive learning models can be employed for longitudinally predicting ADAS-cog scores.Methods: Multivariate regression techniques were employed to model baseline multi-modal data (demographics, neuroimaging, and cerebrospinal ﬂuid based markers, and genetic factors) and future ADAS-cog scores. Prediction models were subjected to repeated cross-validation and the resulting mean absolute error and cross-validated correlation of the model assessed.Results: Prediction models on multi-modal data outperformed single modal data up to 36 months. Incorporating baseline ADAS-cog scores to prediction models marginally improved predictive performance.Conclusions: Future ADAS-cog scores were successfully estimated via predictive learning aiding clinicians in identifying those at greater risk of decline and apply interventions at an earlier disease stage and inform likely future disease progression in individuals enrolled in AD clinical trials.
Joint salvage and biological repair of massive-cavity bone defects after extensive curettage of giant cell tumor around the knee with vascularized fibular autograft and cancellous allograft
Authors: Yang Yun-Fa , Gao Xiao-Sheng , Wang Jian-Wei , Xu Zhong-He 
Published: July 2020

Abstract
Background: Treatment of giant cell tumor (GCT) around knee remains challenging because GCT is prone to recurrence and metastasis. Herein, we reported on our clinical experience with knee joint salvage and biological repair of massive-cavity bone defects after extensive curettage of GCT around the knee with vascularized fibular autograft and cancellous allograft in 12 patients.Methods: All the patients underwent clinical evaluation, plain radiography and/or magnetic resonance imaging (MRI) of the knee right after admission. Their joint function was preserved, and the massive-cavity bone defects were repaired by vascularized fibular autografts and cancellous allograft after extensive curettage of GCT around the knee. All the patients were evaluated through clinical examinations, plain radiography of the knee and chest, and Musculoskeletal Tumor Society (MSTS) scores of the lower extremity in the follow-ups.Results: The follow-up duration ranged from 1.5 years to 12.0 years (mean 4.2 years). There were no local recurrences or lung metastasis in any of the 12 patients at the last follow-up. Ten patients had no pain or experienced occasional pain, and nine were able to resume their previous work. The mean range-of-motion of knee flexion was 117°, and the extension was -6°. The mean MSTS score was 24.7, and a total of 10 patients had excellent or good MSTS scores. Conclusion: Knee joint salvage and biological repair of massive-cavity bone defects could be achieved after extensive curettage with vascularized fibular autograft and cancellous allograft in patients with GCT around the knee.
Pauwels screw combined inverted triangle cannulated screws for the treatment of Pauwels type-III femoral neck fracture:- a new surgical method based on the morphology of the fracture
Authors: Xue Gang , Chen Siting , Zhou Maosheng , Wang Zhengyu , Wan Liangliang , Xie JiaBing , Yang Min 
Published: July 2020

Abstract
Background: Femoral neck fracture is a serious injury in adults with significant functional consequences.Internal fixtion is an established treatment for femoral neck fractures in young adult patients .However, the risk of complications following fixation has plague orthopedists for decades.In all femoral neck fracture ,Pauwels type-III femoral neck fractures with a large vertical shear force were more likely to cause clinical failure.The aim of this study is to intruduce a new surgical technique for the treatment of this fracture according to the morphology of the fracture using the combination of Pauwels screw and inverted triangle cannulated screws(PSTCS)，and report the clinical effects in a prospective cohort of 14 patients.Methods: From June 2017 to June 2019, a total of 14 patients (8 males and 6 females ) with Pauwels type-III femoral neck fracture underwent the surgical treatment of PSTCS according to the morphologyical types of fracture in clinical operation in our department. Patients were allowed to perform contraction exercises of quadriceps femoris on postoperative day1, passive flexion and extension exercises postoperative day 2 onwards, and active flexion and extension exercises postoperative day 7 onwards. Patients were allowed for partial-weight bearing walking postoperative 1month onwards with axillary crutches. After one month AP and lateral radiograph were taken. Full ambulation was permitted until the presence of radiological consolidation.Intraoperative (Operative duration,Blood loss),postoperation(Fracture union time,The Harris Hip Score,Visual analog score) were record for comparison.Results: All patients were followed up for at least 12 months.In the end point of the follow up ,12 of the 14 patients (85.7%)with pauwels type-III femoral neck fractures had achieved satisfactory hip function. Operative duration was a mean of 49.5 ±10.4 minutes.Intraoperative blood loss was between 26 and 110 ml (mean, 44.4±20.6ml) and without intraoperative blood transfusion . The Harris Hip Score was between 64 and 95(mean,87.6±8.9).Fracture healing was observed in all patients according to the X-ray . No internal fixation was loosened or screw cut-out in the follow-up period.The femoral head necrosis was observed in 1 patient in this study. In addition, there was no obvious fracture displacement, femoral neck shortening, or hip varus. Conclusions: The study showed that PSTCS can provide a satisfactory clinical outcomes for treatment of pauwels type-III femoral neck fractures.Most patients (85.7%) achieved good or excellent results with the use of PSTCS,which appears to be a reliable method and provides another choice for this problematic fracture.The new internal fixation method of PSTCS can provide mechanical stability and promote fracture healing
Connective tissue remodelling is differently modulated by Tocilizumab versus Methotrexate monotherapy in patients with early Rheumatoid Arthritis: the AMBITION study
Authors: Drobinski Patryk J. , Bay-Jensen Anne C. , Karsdal Morten A. , Siebuhr Anne S. , Sardar Samra 
Published: July 2020

Abstract
Objective: Associations between Rheumatoid Arthritis (RA) and effect of treatment at the tissue levels are poorly understood. We investigated the scope of released extracellular matrix (ECM) metabolites as a consequence of tissue remodeling in patients treated with Methotrexate (MTX) and Tocilizumab (TCZ) compared to placebo.Methods: Tissue metabolites from 387 RA patients treated with either TCZ (8 mg/kg) or MTX monotherapy (7.5–20 mg/kg) were measured at baseline and 8-weeks sera by validated ELISA assays. The levels of collagen biomarkers (C1M, C2M, C3M and C4M) together with C-reactive protein (CRP) and CRP metabolite (CRPM) were investigated. Baseline levels of biomarkers have been compared with 72 age and gender matched healthy controls. Comparison between treatment and response groups were done by ANCOVA, Spearman’s correlation and Logistic regression adjusted for age, gender, BMI and disease duration.Results: C1M and C3M, were significantly (P < 0.05) inhibited by TCZ and C3M by MTX (P < 0.01) compared to placebo. C1M and C3M inhibition with TCZ was respectively 23% and 16% greater than that of MTX (P < 0.01 and P < 0.0001). C4M was inhibited by TCZ and MTX, but the effect of TCZ was 22% greater than MTX (P < 0.0001). TCZ and MTX had minimal effect on C2M levels. MTX had no effect on CRP and CRPM, whereas TCZ reduced their levels to 69% and 27% from baseline. Investigated biomarkers revealed a significant (P < 0.05) differences in biomarker profiles of MTX ACR50 treatment responders and non-responders. Change to week 8 in levels of C3M, C4M, CRP and CRPM in MTX patients correlated significantly (rho = 0.41 to 0.18, P < 0.0001 to 0.039) with change in disease activity (DAS28) at week 8, 16 and 24, whereas only CRP in TCZ patients (rho = 0.32 to 0.21, P < 0.0001 to 0.01).Conclusion: Patients receiving TCZ treatment for 8 weeks had higher suppression of tissue remodeling and inflammatory biomarkers over patients treated with MTX. Measured biomarkers enabled for a discrimination of biomarker profiles of ACR50 treatment responding patients and identification of those who benefit at the early time-point. Week 8 change in levels of C3M, C4M, CRP and CRPM significantly predicted clinical response to treatment and correlated with DAS28 at all time-points.
Independent and interactive effect of sitting time and physical activity on prevalence of hyperuricemia: the Henan Rural Cohort Study
Authors: Dong Xiaokang , Li Yuqian , Zhang Lulu , Liu Xiaotian , Tu Runqi , Wang Yikang , Li Ruiying , Li Linlin , Hou Jian , Mao Zhenxing , Huo Wenqian , Wang Chongjian 
Published: July 2020

Abstract
BackgroundThere are few studies on the hyperuricemia (HUA) and moderate to vigorous intensity physical activity (PA) and also hardly regarding sitting time (ST). The purpose of this study was to examine the independent and interactive association of PA and ST with HUA.MethodsA cross-sectional analysis was performed on 38,855 participants (aged 18-79) enrolled from the Henan Rural Cohort Study at baseline (2015 to 2017). PA and ST levels were assessed by using the International Physical Activity Questionnaire (IPAQ). HUA was defined as a serum uric acid level of >7.0 mg/dL for males and >6.0 mg/dL for females. Multivariable logistic regression and linear regression models were applied to examine the independent association between PA or ST and HUA and serum uric acid level. Interaction plots were used to visualize the interaction effects of PA and ST on HUA.ResultsPA level was inversely related with serum uric acid level (β: -0.09, 95% confidence interval (CI): -0.16, -0.02), but ST was positively related with uric acid level (β: 2.04, 95% CI:1.82, 2.26). Metabolic equivalent (MET-hour/day) was associated with decreased prevalence of HUA (odds ratio (OR): 0.98, 95% CI: 0.96, 0.99), while per hour increased for ST was associated with increased HUA (OR:1.05, 95% CI: 1.04, 1.06). The interaction of PA and ST was significant (P<0.001).ConclusionExposure to higher ST was independently related to increased prevalence of HUA, while vigorous PA with a decreased HUA prevalence. Meanwhile, higher daily ST might attenuate the protective effect of PA on HUA.Clinical Trial RegistrationThe Henan Rural Cohort Study has been registered at Chinese Clinical Trial Register (Registration number: ChiCTR-OOC-15006699). http://www.chictr.org.cn/showproj.aspx?proj=11375