bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temperature-dependent infrared ellipsometry of Mo-doped VO2 thin films across the insulator to metal transition
Authors: Amador-Alvarado S. , Flores-Camacho J. M. , Solís-Zamudio A. , Castro-García R. , Pérez-Huerta J. S. , Antúnez-Cerón E. , Ortega-Gallegos J. , Madrigal-Melchor J. , Agarwal V. , Ariza-Flores D. 
Published: May 2020

AbstractWe present a spectroscopic ellipsometry study of Mo-doped VO2 thin films deposited on silicon substrates for the mid-infrared range. The dielectric functions and conductivity were extracted from analytical fittings of Ψ and Δ ellipsometric angles showing a strong dependence on the dopant concentration and the temperature. Insulator-to-metal transition (IMT) temperature is found to decrease linearly with increasing doping level. A correction to the classical Drude model (termed Drude-Smith) has been shown to provide excellent fits to the experimental measurements of dielectric constants of doped/undoped films and the extracted parameters offer an adequate explanation for the IMT based on the carriers backscattering across the percolation transition. The smoother IMT observed in the hysteresis loops as the doping concentration is increased, is explained by charge density accumulation, which we quantify through the integral of optical conductivity. In addition, we describe the physics behind a localized Fano resonance that has not yet been demonstrated and explained in the literature for doped/undoped VO2 films.
Mortality of a large wide-ranging mammal largely caused by anthropogenic activities
Authors: Gantchoff M. G. , Hill J. E. , Kellner K. F. , Fowler N. L. , Petroelje T. R. , Conlee L. , Beyer D. E. , Belant J. L. 
Published: May 2020

AbstractWith efforts to restore large mammal populations following extirpations, it is vital to quantify how they are impacted by human activities and gain insights into population dynamics in relation to conservation goals. Our objective was to characterize cause-specific mortality of black bears (Ursus americanus) throughout their range. We first quantified cause-specific mortality for 247 black bears in one harvested and two non-harvested populations. We then simulated a small recolonizing population with and without anthropogenic mortality. Lastly, we conducted a meta-analysis of all published black bear mortality studies throughout North America (31 studies of 2630 bears). We found anthropogenic mortality was greater than natural mortality, non-harvest anthropogenic mortality (e.g. poaching, defense of property, etc.) was greater in non-harvested populations, and harvesting was one of the major causes of mortality for bears throughout their range. Our simulation indicated that removing anthropogenic mortality increased population size by an average of 23% in 15 years. We demonstrated that bears are exposed to high levels of anthropogenic mortality, and the potential for human activities to slow population growth in expanding populations. Management and conservation of wide-ranging mammals will depend on holistic strategies that integrate ecological factors with socio-economic issues to achieve successful conservation and coexistence.
Three-dimensional pore characterization of intact loess and compacted loess with micron scale computed tomography and mercury intrusion porosimetry
Authors: Zhang Linxin , Qi Shengwen , Ma Lina , Guo Songfeng , Li Zhiqing , Li Guoliang , Yang Jijin , Zou Yu , Li Tonglu , Hou Xiaokun 
Published: May 2020

AbstractThe pore structure is one of the most important properties of soil, which can directly affect the other properties such as water content, permeability and strength. It is of great significance to study the soil pore structure for agricultural cultivation, water and soil conservation and engineering construction. This paper investigates the 3D pore characterization of intact loess and four kinds of compacted loess (with different dry density) in northwest China. Micro scale computed tomography and mercury intrusion porosimetry tests were performed to get the porosity, specific surface area, pore size distribution, connected pores content and isolated pores content of different samples. Results show that the intact loess has more connected pores than the compacted loess, and the compacted loess whose dry density appears to be modelled well still have different pore structure with the intact loess. In addition, as the compactness increasing, the large pores (>13 μm) were firstly broken into medium pores (8~13 μm) and some small pores (<8 μm) until the pore structure was close to the natural structure of the intact loess, after that medium pores began to be broken into small pores.
Opposing effects of acute and repeated nicotine exposure on boldness in zebrafish
Authors: Dean Rachel , Duperreault Erika , Newton Dustin , Krook Jeffrey , Ingraham Erica , Gallup Joshua , Franczak Brian C. , Hamilton Trevor J. 
Published: May 2020

AbstractNicotine is an addictive compound that activates neuronal nicotinic acetylcholine receptors (nAChRs) and causes behavioural effects that vary with dose, schedule of administration, and animal model. In zebrafish (Danio rerio), acute doses of nicotine have been consistently found to have anxiolytic properties, whereas, chronic exposure elicits anxiogenic effects. To date, however, studies on repeated nicotine administration and the effects of nicotine withdrawal have not been well explored using this model. In this study, we administered nicotine with three different dosing regimens: 1. Single exposures of a “high” dose (25, 50, 100, or 400 mg/L) for 3 minutes. 2. Single exposures to a “low” dose (2.5, 5, or 20 mg/L) for one hour. 3. Repeated one-hour exposure to a “low” dose (2.5, 5, or 20 mg/L) for 21 days. The novel object approach test was used to examine boldness based on the tendency of the fish to explore a novel object. Acutely, nicotine significantly increased the time spent approaching the object with both three-minute and onehour durations of exposure, indicating increased boldness. Conversely, after repeated nicotine exposure for 21 days, fish spent less time approaching the object suggesting a decrease in boldness. Distance moved was unaffected one hour after repeated nicotine exposure, yet decreased after a two-day withdrawal period. Our work suggests that nicotine can have opposing effects on boldness that vary based on dosage and schedule of exposure.
Using Artificial Intelligence and Novel Polynomials to Predict Subjective Refraction
Authors: Rampat Radhika , Debellemanière Guillaume , Malet Jacques , Gatinel Damien 
Published: May 2020

AbstractThis work aimed to use artificial intelligence to predict subjective refraction from wavefront aberrometry data processed with a novel polynomial decomposition basis. Subjective refraction was converted to power vectors (M, J0, J45). Three gradient boosted trees (XGBoost) algorithms were trained to predict each power vector using data from 3729 eyes. The model was validated by predicting subjective refraction power vectors of 350 other eyes, unknown to the model. The machine learning models were significantly better than the paraxial matching method for producing a spectacle correction, resulting in a mean absolute error of 0.301 ± 0.252 Diopters (D) for the M vector, 0.120 ± 0.094 D for the J0 vector and 0.094 ± 0.084 D for the J45 vector. Our results suggest that subjective refraction can be accurately and precisely predicted from novel polynomial wavefront data using machine learning algorithms. We anticipate that the combination of machine learning and aberrometry based on this novel wavefront decomposition basis will aid the development of refined algorithms which could become a new gold standard to predict refraction objectively.
A novel exopolysaccharide-producing and long-chain n-alkane degrading bacterium Bacillus licheniformis strain DM-1 with potential application for in-situ enhanced oil recovery
Authors: Fan Yanhui , Wang Jun , Gao Chunming , Zhang Yumiao , Du Wen 
Published: May 2020

AbstractA novel Bacillus licheniformis strain (DM-1) was isolated from a mature reservoir in Dagang oilfield of China. DM-1 showed unique properties to utilize petroleum hydrocarbons and agroindustrial by-product (molasses) for exopolysaccharide (EPS) production under oil recovery conditions. The DM-1 EPS was proven to be a proteoglycan with a molecular weight of 568 kDa. The EPS showed shear thinning properties and had high viscosities at dilute concentrations (<1%, w/v), high salinities, and elevated temperatures. Strain DM-1 could degrade long-chain n-alkanes up to C36. Viscosity reduction test have shown that the viscosity of the crude oil was reduced by 40% compared with that before DM-1 treatment. Sand pack flooding test results under simulated reservoir conditions have shown that the enhanced oil recovery efficiency was 19.2% after 7 days of in-situ bioaugmentation with B. licheniformis DM-1. The obtained results indicate that strain DM-1 is a promising candidate for in situ microbial enhanced oil recovery (MEOR).
Characteristics and outcomes of acute-on-chronic liver failure patients with or without cirrhosis using two criteria
Authors: Dong Xiaotian , He Jianqin , Chen Wenyi , Su Rong , Xu Yanping , Sheng Xinyu , Li Lanjuan , Cao Hongcui 
Published: May 2020

AbstractThe aim of the study was to identify the characteristics and outcomes in acute-on-chronic liver failure (ACLF) patients with or without cirrhosis using two criteria. Patients with acute deterioration of chronic hepatic disease or acute decompensation of cirrhosis were included retrospectively from April 10, 2016 to April 10, 2019. European Association for the Study of the Liver-chronic liver failure (EASL-CLIF) criterion except for consideration of cirrhosis and Chinese Group on the Study of Severe Hepatitis B (COSSH) criterion were used. Clinical features, laboratory data and survival curves were compared between the ACLF patients with and without cirrhosis. A total of 799 patients were included. Among them, 328 had COSSH and EASL ACLF, 197 had COSSH alone, and 104 had EASL alone. There were 11.6% more ACLF with COSSH criterion. Furthermore, EASL ACLF patients with non-cirrhosis vs. cirrhosis had different laboratory characteristics: ALT (423 vs. 154, p < 0.001), AST (303 vs. 157, p < 0.001), γ-GT (86 vs. 75, p < 0.01), and INR (2.7 vs. 2.6, p < 0.001) were significantly higher but creatinine (71 vs. 77, p < 0.01) were significantly lower; but importantly there was no statistical changes between non-cirrhosis and cirrhosis in EASL ACLF patients on 28-day (p = 0.398) and 90-day (p = 0.376) survival curves. However, 90-day (p = 0.030) survival curve was different between non-cirrhosis and cirrhosis in COSSH ACLF patients. COSSH ACLF score (auROC = 0.778 or 0.792, 95%CI 0.706–0.839 or 0.721–0.851) displayed the better prognostic ability for EASL ACLF patients with non-cirrhosis, but CLIF-C ACLF score (auROC = 0.757 or 0.796, 95%CI 0.701–0.807 or 0.743–0.843) still was the best prognostic scoring system in EASL ACLF patients with cirrhosis. In conclusions, EASL definition exhibited better performance on homogeneous identification of ACLF regardless of cirrhosis or non-cirrhosis. And COSSH ACLF score displayed the better prognostic ability for EASL ACLF patients without cirrhosis.
Enhancement of guinea pig cytomegalovirus infection by two endogenously expressed components of the pentameric glycoprotein complex in epithelial cells
Authors: Okumura Misaki , Matsuura-Miura Miku , Makino Reina , Miura Takuya , Noguchi Kazuma , Majima Ryuichi , Koshizuka Tetsuo , Inoue Naoki 
Published: May 2020

AbstractA better understanding of the mechanisms underlying cell tropisms and the efficiency of viral infection is critical for the development of vaccines and antiviral drugs for viral diseases. In this study, we worked on the entry mechanisms of guinea pig cytomegalovirus and found that endogenous expression of a combination of two components (GP131 and GP133) of the pentameric glycoprotein complex, which is required for non-fibroblast cell tropisms, enhanced viral infection more than 10-fold. In addition, D138A alteration in GP131 increased this enhancement by an additional 10-fold. Although differences in the efficiency of viral infection among various cell types are usually explained by differences in viral entry or traffic processes, our experimental evidences dismissed such possibilities. Instead, our findings that i) endogenous expression of GP131 and GP133 after nuclear delivery of viral DNA still enhanced infection and ii) an HDAC inhibitor overcame the need of the endogenous expression led us to hypothesize a novel mechanism that controls the efficiency of viral infection through the activation of gene expression from viral DNA delivered to the nuclei. Further studies of this unexpected phenomena warrant to understand novel but also general mechanisms for cell tropisms of viral infection and determinants that control infection efficiency.
The Hepatic Plasma Membrane Citrate Transporter NaCT (SLC13A5) as a Molecular Target for Metformin
Authors: Kopel Jonathan , Higuchi Kei , Ristic Bojana , Sato Toshihiro , Ramachandran Sabarish , Ganapathy Vadivel 
Published: May 2020

AbstractMetformin is the first-line treatment for type 2 diabetes. Inhibition of hepatic gluconeogenesis is the primary contributor to its anti-diabetic effect. Metformin inhibits complex I and α-glycerophosphate shuttle, and the resultant increase in cytoplasmic NADH/NAD+ ratio diverts glucose precursors away from gluconeogenesis. These actions depend on metformin-mediated activation of AMP kinase (AMPK). Here we report on a hitherto unknown mechanism. Metformin inhibits the expression of the plasma membrane citrate transporter NaCT in HepG2 cells and decreases cellular levels of citrate. 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR), an AMPK activator, elicits a similar effect. The process involves a decrease in maximal velocity with no change in substrate affinity. The decrease in NaCT expression is associated with decreased mRNA levels. AMPK inhibits mTOR, and the mTOR inhibitor rapamycin also decreases NaCT expression. The transcription factor downstream of AMPK that is relevant to cAMP signaling is CREB; decreased levels of phospho-CREB seem to mediate the observed effects of metformin on NaCT. Citrate is known to suppress glycolysis by inhibiting phosphofructokinase-1 and activate gluconeogenesis by stimulating fructose-1,6-bisphophatase; therefore, the decrease in cellular levels of citrate would stimulate glycolysis and inhibit gluconeogenesis. These studies uncover a novel mechanism for the anti-diabetic actions of metformin.
Development of 60-GHz millimeter wave, electromagnetic bandgap ground planes for multiple-input multiple-output antenna applications
Authors: Ullah Sana , Yeo Woon-Hong , Kim Hongjoon , Yoo Hyoungsuk 
Published: May 2020

AbstractFor 60-GHz band communications, both the mutual coupling and transmission distance restrict the performance of a multiple-input multiple-output (MIMO) antenna array. Several studies presented different types of meta-materials and electromagnetic bandgap (EBG) structures to improve the performance of a MIMO antenna array at the 60-GHz band. In this paper, we presented the four-element MIMO patch antenna with different types of EBG structures for the millimeter wave (mmW)communications at the 60-GHz unlicensed industrial, scientific, and medical band. The single element of the MIMO antenna array covered the mmW band from 57 GHz to 63 GHz having the dimensions of 1.3 mm × 1.8 mm × 0.1 mm. We developed a set of square-shaped, cross-shaped, and complex-slotted EBG ground planes between the antenna elements for the performance improvement. All the three EBG ground planes provided significant coupling reduction between the mmW MIMO antenna elements. The proposed EBG structures exhibited wide bandgap characteristics and improved scattering parameters in the desired frequency band. In contrast with the cross- and complex-slotted, the square-shaped EBG structure substantially improved the overall gain of MIMO antenna array. In addition, the square-shaped EBG reformed the maximum beam and enhanced the far-field gain pattern in the desired direction. Experimental results conducted with the fabricated prototypes showed a good agreement with the simulation results and adequately covered the 60-GHz band. The low-profile and salient features of the proposed MIMO antenna array shows the potential for on-chip applications at 60 GHz.
Genetically modified crops are superior in their nitrogen use efficiency-A meta-analysis of three major cereals
Authors: Li Mengjiao , Xu Jili , Gao Zhiyuan , Tian Hui , Gao Yajun , Kariman Khalil 
Published: May 2020

AbstractIt is currently uncertain to what extent genetic transformations of strategic crops (targeting diverse traits) have improved their N use efficiency (NUE), and what the key factors affecting their NUE are. Based on data collected from 130 publications, the effect sizes of genetic transformations and the key factors influencing NUE for three major cereal crops (rice, maize, and wheat), were investigated using a meta-analysis approach. Genetic transformations increased yield, shoot biomass, N uptake efficiency (NUpE), and partial factor productivity of N (PFPN) in the crops, but decreased shoot NUE (SNUE) and grain NUE (GNUE). Transporter genes improved yield and NUE parameters more efficiently, than did the other gene types. The effect sizes for some NUE parameters varied according to crop species and experimental conditions but did not differ between the overexpression and ectopic expression methods. Most effect sizes did not correlate with gene overexpression levels. These results indicate a promising potential of genetic transformations approaches for improving certain NUE parameters.
Synapse elimination activates a coordinated homeostatic presynaptic response in an autaptic circuit
Authors: Velasco Cecilia D. , Llobet Artur 
Published: May 2020

AbstractThe number of synapses present in a neuronal circuit is not fixed. Neurons must compensate for changes in connectivity caused by synaptic pruning, learning processes or pathological conditions through the constant adjustment of the baseline level of neurotransmission. Here, we show that cholinergic neurons grown in an autaptic circuit in the absence of glia sense the loss of half of their synaptic contacts triggered by exposure to peptide p4.2, a C-terminal fragment of SPARC. Synaptic elimination is driven by a reorganization of the periodic F-actin cytoskeleton present along neurites, and occurs without altering the density of postsynaptic receptors. Neurons recover baseline neurotransmission through a homeostatic presynaptic response that consists of the coordinated activation of rapid synapse formation and an overall potentiation of presynaptic calcium influx. These results demonstrate that neurons establishing autaptic connections continuously sense and adjust their synaptic output by tweaking the number of functional contacts and neurotransmitter release probability.
Phosphoproteomics identifies dual-site phosphorylation in an extended basophilic motif regulating FILIP1-mediated degradation of filamin-C
Authors: Reimann Lena , Schwäble Anja N. , Fricke Anna L. , Mühlhäuser Wignand W. D. , Leber Yvonne , Lohanadan Keerthika , Puchinger Martin G. , Schäuble Sascha , Faessler Erik , Wiese Heike , Reichenbach Christa , Knapp Bettina , Peikert Christian D. , Drepper Friedel , Hahn Udo , Kreutz Clemens , van der Ven Peter F. M. , Radziwill Gerald , Djinović-Carugo Kristina , Fürst Dieter O. , Warscheid Bettina 
Published: May 2020

AbstractThe PI3K/Akt pathway promotes skeletal muscle growth and myogenic differentiation. Although its importance in skeletal muscle biology is well documented, many of its substrates remain to be identified. We here studied PI3K/Akt signaling in contracting skeletal muscle cells by quantitative phosphoproteomics. We identified the extended basophilic phosphosite motif RxRxxp[S/T]xxp[S/T] in various proteins including filamin-C (FLNc). Importantly, this extended motif, located in a unique insert in Ig-like domain 20 of FLNc, is doubly phosphorylated. The protein kinases responsible for this dual-site phosphorylation are Akt and PKCα. Proximity proteomics and interaction analysis identified filamin A-interacting protein 1 (FILIP1) as direct FLNc binding partner. FILIP1 binding induces filamin degradation, thereby negatively regulating its function. Here, dual-site phosphorylation of FLNc not only reduces FILIP1 binding, providing a mechanism to shield FLNc from FILIP1-mediated degradation, but also enables fast dynamics of FLNc necessary for its function as signaling adaptor in cross-striated muscle cells.
Potential role of miR-155-5p in fat deposition and skeletal muscle development of chicken
Authors: Xu Sifan , Chang Yang , Wu Guanxian , Zhang Wanting , Man Chaolai 
Published: May 2020

Abstract
miR-155 has multiple functions in many physiological and pathological processes. However, little is known about the expression characteristics of avian miR-155. In the present study, partial pri-miR-155 sequences were cloned from AA+ broiler, Sanhuang broiler and Hy-Line Brown layer, respectively. Stem–loop qRT-PCR was performed to detect the miR-155-5p spatiotemporal expression profiles of each chicken breed, and the target genes of miR-155-5p were predicted in Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The results showed that the partial pri-miR-155 sequences of different breeds of chicken were high conserved. The expression patterns of miR-155-5p between broiler and layer were basically similar, and miR-155-5p was expressed highly in immune related tissues (spleen, thymus and bursa). In the same old chicken (14 days old), miR-155-5p expression activity of fat tissue all had higher level in the three chicken breeds, but the expression activities in skeletal muscle of broilers were significantly lower than that of layer (P&lt;0.05). In different development stages of Hy-Line Brown layer, miR-155-5p expression activities in skeletal muscle of 14-day-old and 10-month-old layers were significantly lower than that of 24-month-old layer (P&lt;0.05). Fat related target genes (ACOX1, ACOT7, FADS1, SCD and HSD17B12) and skeletal muscle related target genes (CCNT2, DMD, CFL2, MAPK14, FLNB, ZBTB18 and CDK5) of miR-155-5p were predicted, respectively. The results indicate that miR-155-5p may be an important factor inhibiting the fat deposition and skeletal muscle development in chicken.