bluuebunny/crossref_metadata_2025_split huggingface dataset:
Apoptosis detection via automated algorithms to analyze biomarker translocation in reporter cells
Authors: Ahmed A. H. Rezwanuddin , Dereli‐Korkut Zeynep , Lee Joanne Haeun , Piracha Sidra , Gilchrist M. Lane , Jiang Xuejun , Wang Sihong 
Published: January 2020

AbstractRapid, efficient, and robust quantitative analyses of dynamic apoptotic events are essential in a high‐throughput screening workflow. Currently used methods have several bottlenecks, specifically, limitations in available fluorophores for downstream assays and misinterpretation of statistical image data analysis. In this study, we developed cytochrome‐C (Cyt‐C) and caspase‐3/‐8 reporter cell lines using lung (PC9) and breast (T47D) cancer cells, and characterized them from the response to apoptotic stimuli. In these two reporter cell lines, the spatial fluorescent signal translocation patterns served as reporters of activations of apoptotic events, such as Cyt‐C release and caspase‐3/‐8 activation. We also developed a vision‐based, tunable, automated algorithm in MATLAB to implement the robust and accurate analysis of signal translocation in single or multiple cells. Construction of the reporter cell lines allows live monitoring of apoptotic events without the need for any other dyes or fixatives. Our algorithmic implementation forgoes the use of simple image statistics for more robust analytics. Our optimized algorithm can achieve a precision greater than 90% and a sensitivity higher than 85%. Combining our automated algorithm with reporter cells bearing a single‐color dye/fluorophore, we expect our approach to become an integral component in the high‐throughput drug screening workflow.
An analysis of environmental sustainability in small & medium‐sized enterprises: Patterns and trends
Authors: Bakos Joseph , Siu Michele , Orengo Adalberto , Kasiri Narges 
Published: January 2020

AbstractOne of the greatest challenges facing the world today is climate change. The need to consistently advance with environmentally sustainable practices in today's businesses is crucial, and businesses are demanded to be more environmentally sustainable every day. This study conducts an analysis of the literature on small and medium‐sized enterprises' (SMEs) environmental sustainability by carefully examining 122 studies from 58 journals published from 2013 to 2019. We investigate the trends in drivers and barriers of sustainability adoption to inform both SMEs managers and policymakers. Our results also classify the reviewed studies on the basis of their methodologies and show the distribution of studies across industry sectors and locations in order to set directions for future research on sustainability practices of SMEs.
Anti‐epidermal growth factor receptor monoclonal antibody plus palliative chemotherapy as a first‐line treatment for recurrent or metastatic nasopharyngeal carcinoma
Authors: Chen Chen , Zhou Yixin , Zhang Xuanye , Fu Sha , Lin Zuan , Fang Wenfeng , Yang Yunpeng , Huang Yan , Zhao Hongyun , Hong Shaodong , Zhang Li 
Published: January 2020

AbstractBackgroundPlatinum‐based chemotherapy is the standard of care as first‐line treatment for recurrent or metastatic nasopharyngeal carcinoma (RM‐NPC); however, the prognosis of patients with RM‐NPC remains poor. The aim of this study was to evaluate the role of anti‐epidermal growth factor receptor (anti‐EGFR) antibody plus chemotherapy for RM‐NPC.MethodsRM‐NPC patients who received first‐line chemotherapy plus an anti‐EGFR antibody were recruited from Sun Yat‐Sen University Cancer Center between July 2007 and November 2017. Survival analyses were performed using the Kaplan‐Meier method with a log‐rank test. A Cox proportional hazards model was used for the multivariate analyses.ResultsA total of 203 patients were enrolled in the present study. The median follow‐up time was 34.3 months (interquartile range: 19.7‐66.5 months). The median progression‐free survival (PFS) was 8.9 months (95% CI: 7.7‐10.0 months) and the median overall survival (OS) was 29.1 months (95% CI: 23.5‐34.6 months). The 1‐, 3‐, and 5‐year PFS and OS rates were 35.5% and 79.6%, 15.2% and 42.5%, and 11.6% and 23.6%, respectively. The objective response rate (ORR) was 67.5% and the disease control rate (DCR) was 91.1%. The multivariate analysis identified the following prognostic factors for PFS: anti‐EGFR agent (P = .010), recurrence/metastasis sequence (P = .016), KPS (P = .017), and combined chemotherapy regimen (P = .015). Independent risk factors for OS included age >43 years (P = .002), Karnofsky performance score ≤80 (P < .001), and higher level of baseline Epstein‐Barr virus (EBV) DNA (P = .008). Leukopenia was the most common adverse event (AE) in this cohort (any grade, 84.2%; grades 3‐4, 43.4%).ConclusionsAnti‐EGFR antibody plus chemotherapy achieved promising antitumor activity with a tolerable toxicity profile in RM‐NPC. Thus, randomized clinical trials are warranted to compare the efficacy of chemotherapy with or without anti‐EGFR antibody in these patients.
Chloropupukeananin and Pestalofone C Regulate Autophagy through AMPK and Glycolytic Pathway
Authors: Guo Longfang , Zhang Caining , Gao Quan , Hou Bolin , Liu Ling , Yang Huaiyi , Jiang Xuejun 
Published: January 2020

AbstractChloropupukeananin (RN56‐6) and Pestalofone C (RN56‐49), isolated from the culture of the plant endophytic fungus Pestalotiopsis fici, have been shown cytotoxic, anti‐HIV, and antimicrobial activities. However, the underlying mechanism of their regulatory roles in autophagy remains unknown. In the present study, we revealed that both compounds increased the formation of autophagosome and enhanced autophagic flux. While RN56‐6 upregulated the expression of HK2, one of the key rate‐limiting enzymes of glycolysis, the inhibition of glycolysis chemically attenuated RN56‐6‐induced autophagy. On the contrary, RN56‐49 downregulated the expression of HK2, while the suppression of glycolysis promoted RN56‐49‐dependent autophagic flux. Moreover, the knockdown of AMPKβ1, a scaffolding subunit of AMPK, decreased autophagy induced by these two compounds. Collectively, these findings revealed that RN56‐6 and RN56‐49 regulated autophagic process through AMPK and glycolytic pathway.
Percutaneous coronary intervention with transcatheter aortic valve replacement makes no difference! None? Really?
Authors: Block Peter C. , Kumar Arnav 
Published: January 2020

Key Points
Unless a patient who needs transcatheter aortic valve replacement (TAVR) presents with an acute coronary syndrome, “routine” percutaneous coronary intervention of coronary stenoses does not improve outcomes, even out to 5 years.
Randomized clinical trials are needed to sort out the best strategies to treat the complex interaction of coronary disease and aortic stenosis, though they are unlikely to be performed.
Without such evidence, patients undergoing TAVR need the judgment of a Heart Team to help strategize whether revascularization for CAD should be performed.
Assessing the risk of an anomalous circumflex artery using balloon aortic valvuloplasty prior to transcatheter aortic valve replacement
Authors: Tabachnick Deborah , Obokhae Brigette , Harrington Katherine , Brown David L. 
Published: January 2020

AbstractAnomalous origin of the left circumflex coronary artery from the right sinus of Valsalva or proximal right coronary artery (RCA) is a well‐known anatomic variation. Although the condition is usually benign, there is risk for compression of the anomalous artery by a prosthetic valve in patients undergoing aortic valve replacement (AVR). In more recent years, balloon aortic valvuloplasty (BAV) has been performed prior to transcatheter aortic valve replacement (TAVR) to serve as a diagnostic tool in the evaluation of symptom relief and procedural risks prior to definitive therapy with TAVR. However, the literature regarding BAV utilization in the assessment of coronary artery anomalies prior to TAVR is scarce. Our case illustrates the importance of performing preoperative BAV to assess the safety of a TAVR procedure in patients with coronary anomalies. Herein, we present a case of a patient who underwent BAV with selective angiography of her anomalous circumflex artery. During balloon inflation, the anomalous circumflex artery was transiently occluded, with complete resolution with balloon deflation. Given these findings, the patient was deemed to be unsuitable for TAVR and offered surgical AVR. This case demonstrates that patients with anomalous coronary circulation may require BAV with selective angiography to fully evaluate risk of coronary occlusion with TAVR.
Efficacy and safety of intravascular lithotripsy for the treatment of peripheral arterial disease: An individual patient‐level pooled data analysis
Authors: Madhavan Mahesh V. , Shahim Bahira , Mena‐Hurtado Carlos , Garcia Lawrence , Crowley Aaron , Parikh Sahil A. 
Published: January 2020

AbstractBackgroundPeripheral arterial disease (PAD) is one of the most common manifestations of atherosclerotic disease worldwide. Peripheral arterial calcification reduces acute success and long‐term patency of endovascular therapy for PAD. Several calcium modification devices are available for use in peripheral interventions. Outcomes after peripheral intravascular lithotripsy (IVL), a novel approach using pulsatile sonic waves to treat luminal and medial calcium in patients with PAD, have not been extensively characterized. Therefore, we sought to perform an individual patient‐level data (IPD) pooled analysis of available studies to evaluate the efficacy and safety of IVL in the treatment of PAD.Methods and resultsWe pooled IPD, including baseline and procedural variables, from five prospective studies which assessed IVL in the treatment of patients with extensive peripheral artery calcification. Final postprocedural percent diameter stenosis (%DS) and procedural angiographic complications were assessed by independent core laboratory. Efficacy endpoints were analyzed using linear mixed effects models and safety endpoints were tabulated overall and by vascular bed. Among 336 patients who underwent endovascular revascularization with use of IVL, there was a significant reduction between pre‐procedural and final %DS of 55.1% (95% confidence interval 53.3–57.0%, p < .0001). Core‐laboratory assessed lesion‐level complications, including flow‐limiting dissections (Types D–F), vessel perforation, distal embolization, thrombus, abrupt closure, and no reflow, occurred in 4/328 (1.22%) of treated lesions.ConclusionsThe present IPD of five prospective studies, marking the largest analysis to date evaluating the use of IVL in significantly calcified PAD lesions, demonstrates this treatment strategy to be both effective and safe.
Complete percutaneous apical access and closure: Short and intermediate term outcomes
Authors: Eng Marvin H. , Kherallah Riyad Yazan , Guerrero Mayra , Greenbaum Adam B. , Frisoli Tiberio , Villablanca Pedro , Wang Dee Dee , Lee James , Wyman Janet , O'Neill William W. 
Published: January 2020

AbstractObjectivesTo examine the safety of utilizing transapical access during structural interventions.BackgroundComplex interventions of the mitral or aortic region sometimes require coaxial forces to orient and deliver devices. Apical access can provide coaxial countertraction for either transseptal or retrograde aortic access. This manuscript describes the single center experience of small bore transapical access.MethodsRetrospective review of cases from 2013 to 2018 at Henry Ford Hospital was performed. Patient demographics and procedure characteristics were abstracted to describe the safety of transapical access using small bore sheaths.ResultsA total 21 cases were performed at Henry Ford, most of them for transcatheter mitral valve replacement (81%). The mean sheath size used was 4.7 ± 0.9 Fr and protamine was used at the end of 57% of cases. All patients received nitinol‐based plugs, 80.1% were from the Amplatz Duct Occluder II type. Four major complications related apical puncture occurred, two pericardial effusions, two hemothorax. Over a median follow time of 430 days (IQR 50–652) a total of five deaths occurred, two related to the procedure and three late deaths with a median time of 362 days (range 205–628 days). No deaths were associated with transapical access. Echocardiographic follow up did not detect any late structural complications from occluder devices.ConclusionsTransapical access and closure with nitinol‐based devices is feasible and facilitates complex interventions where coaxial forces are need for device delivery and alignment. The most common complication is bleeding and this should be kept in perspective when treating high‐risk patients.
Compression of the left mainstem bronchus by patent ductus arteriosus in neonates under consideration for ductal stenting
Authors: Smith Christopher L. , Saul David , Goldfarb Samuel B. , Biko David M. , O'Byrne Michael L. 
Published: January 2020

AbstractStent angioplasty of patent ductus arteriosus has been shown to be a viable alternative to operative shunt placement in cyanotic neonates. With broader implementation of this strategy, novel complications are bound to arise. We present a series of cases evaluated for ductal stent angioplasty in which a dilated and torturous ductus arteriosus compressed the left mainstem bronchus. After reviewing our recent experience with ductal stenting and isolated Blalock‐Taussig shunts, our best estimate of the incidence of bronchial compression by the dilated ductus is 4.6% (3/64, 95% confidence interval 1.0–12.9%). Awareness of the airway and other nonvascular contents of the thorax is an important consideration prior to ductal stenting.
Intravascular lithotripsy to treat an underexpanded coronary stent: 4‐Month angiographic and OCT follow‐up
Authors: Alawami Mohammed , Thirunavukarasu Sharmaine , Ahmed Javed , El‐Omar Magdi 
Published: January 2020

AbstractWe report the case of a 79‐year‐old man with stable angina who underwent percutaneous coronary intervention to a severe and calcified left circumflex lesion. Despite extensive preparation of the lesion with high‐pressure balloon predilatation and rotablation, the implanted stent was grossly underexpanded and failed to respond to high‐pressure balloon postdilatation. The patient was readmitted 6 weeks later for intravascular lithotripsy that resulted in excellent stent expansion. Coronary angiography with optical coherence tomography 4 months later revealed sustained acute lumen gain with no evidence of stent recoil or in‐stent restenosis.
Hollow and Hierarchical Cobalt–Metal Organic Framework@CoCr2O4 Microplate Array as a Battery‐Type Electrode for High‐Performance Hybrid Supercapacitors
Authors: Li Qin , Zhou Jiaojiao , Zhao Shihang , Li Yanli , Chen Chen , Tao Kai , Liu Rui , Han Lei 
Published: January 2020

AbstractHollow metal‐organic frameworks (MOFs) and their derived materials have shown fascinating structural complexity, such as diverse morphology, tunable porosity and hierarchical structure, which largely extends their intrinsic applications, especially in electrochemical energy storage and conversion. Herein, a hollow and hierarchical Co–MOF@CoCr2O4 microplate array on nickel foam was successfully prepared through a simple one‐step in situ conversion strategy, in which Co–MOF served both as self‐supporting etching template and as a self‐sacrificing ion‐exchange template. When used as a battery‐type electrode for advanced hybrid supercapacitors, the Co–MOF@CoCr2O4 composite expressed a high specific capacity of 596.8 C g−1 at 0.4 A g−1, excellent rate properties (72.6 % retention rate at 10 A g−1 compared with the initial capacitance at 0.4 A g−1) and good cycling stability (85.1 % retention over 5000 cycles). Furthermore, a fabricated hybrid supercapacitor device reached a high energy density of 0.4 mWh cm−2 (34.36 Wh kg−1) at a power density of 2.34 mW cm−2 (201.03 W kg−1), and superior cycle stability (96.2 % retention over 5000 cycles). These enhanced electrochemical behaviors indicated that this advanced approach can be extended to design various hollow and hierarchical structural MOF/bimetallic‐oxide‐based composite arrays for high‐performance hybrid supercapacitors.
Synthesis of CoS2 Nanoparticles/Nitrogen‐Doped Graphitic Carbon/Carbon Nanotubes Composite as an Advanced Anode for Sodium‐Ion Batteries
Authors: Li Yong , Guo Rui , Sun Yanting , Wang Yong , Liu Wen , Pei Haijuan , Zhao Hongbin , Zhang Jiujun , Ye Daixin , Xie Jingying , Kong Jilie 
Published: January 2020

AbstractCobalt sulfide (CoS2) is a promising anode material for high‐performance sodium‐ion batteries (SIBs) because of its low cost, excellent electrical conductivity, and high theoretical capacity. The practical implementation of a CoS2 anode, however, is still hindered by fast capacity decay and low power density caused by its huge volume expansion during the charging/discharging process. Herein, we developed a low‐cost, facile and large‐scale method to fabricate a nitrogen‐doped carbon‐layer‐constrained structured CoS2/carbon nanotubes (NC@CoS2@CNTs) hybrid with a novel morphology for high‐performance SIBs. The hybrid electrode has reversible specific capacities of 669 and 334 mAh g−1 at the current densities of 200 mA g−1 and 5000 mA g−1, respectively, and an unprecedented cycling stability of approximately 0.014 % capacity decay per cycle over 1000 cycles at 3000 mA g−1. The balanced high electrochemical performance for the hybrid electrode can be attributed to the synergetic effect among the CoS2 nanoparticles, N‐doped carbon coating and in situ grown CNTs. The results demonstrate that the CoS2 can be well utilized with the N‐doped carbon/carbon nanotubes hybrid material structure, which provides a facile approach to realize excellent performance for practical applications.
Synthesis, Modification, and Biological Evaluation of a Library of Novel Water‐Soluble Thiopyridone‐Based Organometallic Complexes and Their Unexpected (Biological) Behavior
Authors: Harringer Sophia , Happl Barbara , Ozenil Marius , Kast Caroline , Hejl Michaela , Wernitznig Debora , Legin Anton A. , Schweikert Andreas , Gajic Natalie , Roller Alexander , Koellensperger Gunda , Jakupec Michael A. , Kandioller Wolfgang , Keppler Bernhard K. 
Published: January 2020

AbstractA series of 16 dinuclear thiopyridone‐based organometallics with excellent water solubility, increased stability and remarkable cytotoxicity were synthesized and characterized. The complexes of this work formed dimeric species featuring a double positive charge in polar protic solvents, accounting for their outstanding solubility in aqueous solution. Most of them displayed higher antiproliferative activity than their parental thiomaltol complex, with unexpected cytotoxicity trends depending on the employed metal center, ligand modification, and cell line. Insights into their behavior in biological systems were gathered by means of amino‐acid interaction studies, cytotoxicity tests in 3D spheroid models, laser ablation, cellular accumulation measurements, as well as cell cycle experiments.
Ambident Nucleophilic Substitution: Understanding Non‐HSAB Behavior through Activation Strain and Conceptual DFT Analyses
Authors: Bettens Tom , Alonso Mercedes , De Proft Frank , Hamlin Trevor A. , Bickelhaupt F. Matthias 
Published: January 2020

AbstractThe ability to understand and predict ambident reactivity is key to the rational design of organic syntheses. An approach to understand trends in ambident reactivity is the hard and soft acids and bases (HSAB) principle. The recent controversy over the general validity of this principle prompted us to investigate the competing gas‐phase SN2 reaction channels of archetypal ambident nucleophiles CN−, OCN−, and SCN− with CH3Cl (SN2@C) and SiH3Cl (SN2@Si), using DFT calculations. Our combined analyses highlight the inability of the HSAB principle to correctly predict the reactivity trends of these simple, model reactions. Instead, we have successfully traced reactivity trends to the canonical orbital‐interaction mechanism and the resulting nucleophile–substrate interaction energy. The HOMO–LUMO orbital interactions set the trend in both SN2@C and SN2@Si reactions. We provide simple rules for predicting the ambident reactivity of nucleophiles based on our Kohn–Sham molecular orbital analysis.
Organocatalytic synthesis of 5‐hydroxyisoxazolidine catalyzed by camphor sulfonyl hydrazines through aza‐Michael addition/cyclization
Authors: Xu Fang‐Fang , Chen Ling‐Yan , Sun Peng , Lv Yixin , Zhang Yan‐Xue , Li Jia‐Yu , Yin Xiaoying , Li Ya 
Published: January 2020

AbstractA series of chiral 5‐hydroxy isoxazolidines has been successfully synthesized through camphor sulfonyl hydrazine‐catalyzed asymmetric aza‐Michael addition reaction between N,O‐protected hydroxyamines and enals. Moderate yields with moderate to good enantioselectivities (up to 96% enantiomeric excess [ee]) were achieved. It provides an alternative asymmetric approach to preparing isoxazolidine derivatives.
New entry to the enantioselective formation of substituted cyclohexenes bearing an all‐carbon quaternary stereogenic center
Authors: Komine Keita , Urayama Yasuhiro , Hosaka Taku , Fukuda Hayato , Hatakeyama Susumi , Ishihara Jun 
Published: January 2020

AbstractEnantioselective formation of cyclohexene derivatives bearing an all‐carbon quaternary stereogenic center is described. The racemic cyclohexenes are readily transformed to chiral substituted cyclohexenes in good yield with excellent enantioselectivity and diastereoselectivity by a palladium‐mediated deracemization. The resulting products are promising synthetic intermediates of biologically active natural products. This protocol provides us with a new entry to the concise and scalable synthesis of multifunctionalized compounds.
Impact of leaflet thrombosis on hemodynamics and clinical outcomes after bioprosthetic aortic valve replacement: A meta‐analysis
Authors: Tian Zixin , Li Tiejun , Ma Shumei 
Published: January 2020

AbstractBackgroundLeaflet thrombosis (LT, also called cusp thrombosis) detected by multidetector computed tomography (MDCT) is common in bioprosthetic aortic valve replacement (bAVR). However, it remains contradictory whether MDCT‐defined LT following bAVR is associated with hemodynamic deterioration and stroke. Thus, we performed the first meta‐analysis to assess hemodynamic outcomes and updated the latest researches on the clinical outcomes of MDCT‐defined LT after bAVR.HypothesisMDCT‐defined LT might be associated with worse hemodynamic and clinical outcomes after bAVR.MethodMEDLINE, EMBASE, Cochrane Library, and ClinicalTrial.gov were searched from inception to 15th April 2019. The fix‐effect model was utilized to calculate odds ratio (OR) and 95% confidence interval (CI). The primary outcomes were hemodynamic stability indexes, including mean pressure gradient (MPG), left ventricular ejection fraction (LVEF), paravalvular leak (PVL), and clinical heart failure. The secondary endpoints were major adverse cardiovascular and cerebrovascular events (MACCEs), which consisted of myocardial infarction, all‐cause death, stroke, and transient ischemic attack (TIA).ResultsTwelve studies with 4820 patients were included. The total prevalence of MDCT‐defined LT was 9.7%. MDCT‐defined LT was associated with a significantly increased risk of MPG (inverse variance 0.43, 95% CI: [0.30, 0.57]), MACCEs (OR 2.43, 95% CI: [1.45, 4.06]), stroke (OR 1.79, 95% CI: [1.03, 3.11]), and TIA (OR 4.09, 95% CI: [1.59, 10.54]). There were no differences for other outcomes.ConclusionsMDCT‐defined LT after bAVR is associated with increased MPG and increased risk of adverse cerebrovascular events, including TIA and stroke. While LVEF, PVL, and clinical heart failure were similar between patient with and without LT.