bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toxicity of three types of arsenolipids: species-specific effects in Caenorhabditis elegans
Authors: Bornhorst Julia , Ebert Franziska , Meyer Sören , Ziemann Vanessa , Xiong Chan , Guttenberger Nikolaus , Raab Andrea , Baesler Jessica , Aschner Michael , Feldmann Jörg , Francesconi Kevin , Raber Georg , Schwerdtle Tanja 
Published: March 2020

Abstract
Although fish and seafood are well known for their nutritional benefits, they contain contaminants that might affect human health. Organic lipid-soluble arsenic species, so called arsenolipids, belong to the emerging contaminants in these food items; their toxicity has yet to be systematically studied. Here, we apply the in vivo model Caenorhabditis elegans to assess the effects of two arsenic-containing hydrocarbons (AsHC), a saturated arsenic-containing fatty acid (AsFA), and an arsenic-containing triacylglyceride (AsTAG) in a whole organism. Although all arsenolipids were highly bioavailable in Caenorhabditis elegans, only the AsHCs were substantially metabolized to thioxylated or shortened metabolic products and induced significant toxicity, affecting both survival and development. Furthermore, the AsHCs were several fold more potent as compared to the toxic reference arsenite. This study clearly indicates the need for a full hazard identification of subclasses of arsenolipids to assess whether they pose a risk to human health.
Molecular detection of methicillin heat-resistant Staphylococcus aureus strains in pasteurized camel milk in Saudi Arabia
Authors: Aljahani Amani H. , Alarjani Khaloud M. , Hassan Zeinab K. , Elkhadragy Manal F. , Ismail Elsayed A. , Al-Masoud Abdulrahman H. , Yehia Hany M. 
Published: March 2020

Abstract
Antibiotic- and heat-resistant bacteria in camel milk is a potential public health problem. Staphylococcus aureus (S. aureus) is an opportunistic pathogen in humans, dairy cattle and camels. We characterized the phenotype and genotype of methicillin-resistant staphylococcal strains recovered from pasteurized and raw camel milk (as control) distributed in the retail markets of Saudi Arabia. Of the 100 samples assessed between March and May 2016, 20 S. aureus isolates were recovered from pasteurized milk, 10 of which were resistant to cefoxitin, and as such, were methicillin-resistant. However, raw camel milk did not contain methicillin-resistant S. aureus (MRSA). Antimicrobial susceptibility tests showed that the resistance ratio for other antibiotics was 60%. We performed a polymerase chain reaction (PCR) assay using primers for the methicillin-resistant gene mecA and nucleotide sequencing to detect and verify the methicillin-resistant strains. Basic local alignment search tool (BLAST) analysis of the gene sequences showed a 96–100% similarity between the resistant isolates and the S. aureus CS100 strain’s mecA gene. Ten of the methicillin-resistant isolates were heat-resistant and were stable at temperatures up to 85°C for 60 s, and three of these were resistant at 90°C for 60 or 90 s. The mean decimal reduction time (D85-value) was 111 s for the ten isolates. Sodium dodecyl sulfate (SDS)/polyacrylamide gel electrophoresis (PAGE) showed that there was no difference in the total protein profiles for the ten methicillin heat-resistant S. aureus (MHRSA) isolates and for S. aureus ATCC 29737. In conclusion, a relatively high percentage of the tested pasteurized camel milk samples contained S. aureus (20%) and MHRSA (10%).
Tumoral PD-1hiCD8+ T cells are partially exhausted and predict favorable outcome in triple-negative breast cancer
Authors: Guo Liang , Cao Chunmei , Goswami Shyamal , Huang Xiaoyan , Ma Linxiaoxi , Guo Yicheng , Yang Benlong , Li Teng , Chi Yayun , Zhang Xiaoming , Wu Jiong 
Published: March 2020

Abstract
Tumor-infiltrating PD-1hi dysfunctional CD8+ T cells have been identified in several tumors but largely unexplored in breast cancer (BC). Here we aimed to extensively explore PD-1hiCD8+ T cells in BC, focusing on the triple-negative BC (TNBC) subtype. Flow cytometry was used to study the phenotypes and functions of CD8+ T-cell subsets in peripheral blood and surgical specimens from treatment-naive BC patients. RNA-seq expression data generated to dissect the molecular features of tumoral PD-1neg, PD-1lo and PD-1hi CD8+ T cells. Further, the associations between tumoral PD-1hi CD8+ T cells and the clinicopathological features of 503 BC patients were explored. Finally, multiplexed immunohistochemistry (mIHC) was performed to evaluate in situ PD-1hiCD8+ T cells on the tissue microarrays (TMAs, n=328) for prognostic assessment and stratification of TNBC patients. PD-1hiCD8+ T cells found readily detectable in tumor tissues but rarely in peripheral blood. These cells shared the phenotypic and molecular features with exhausted and tissue-resident memory T cells (TRM) with a skewed TCR repertoire involvement. Interestingly, PD-1hiCD8+ T cells are in the state of exhaustion characterized by higher T-BET and reduced EOMES expression. PD-1hiCD8+ T cells found preferentially enriched within solid tumors, but predominant stromal infiltration of PD-1hiCD8+ T subset was associated with improved survival in TNBC patients. Taken together, tumoral PD-1hiCD8+ T-cell subpopulation in BC is partially exhausted, and their abundance signifies ‘hot’ immune status with favorable outcomes. Reinvigorating this population may provide further therapeutic opportunities in TNBC patients.
Renal functional effects of the highly selective AT2R agonist, β-Pro7 Ang III, in normotensive rats
Authors: Krause Lucinda M. Hilliard , Kemp Brandon A. , Tan Amanda Suan Jui , Jones Emma S. , Del Borgo Mark P. , Aguilar Marie-Isabel , Denton Kate M. , Carey Robert M. , Widdop Robert E. 
Published: March 2020

AbstractRecently, we designed a group of peptides by sequential substitution of the naturally occurring α-amino acid throughout the Ang III peptide sequence with the corresponding β-amino acid. β-Amino acid substitution at the proline residue of Ang III (β-Pro7-Ang III) resulted in a highly selective AT2R ligand, demonstrating remarkable selectivity for the AT2R in both binding and functional studies. To provide additional functional evidence for the suitability of β-Pro7 Ang III as a novel AT2R agonist, we tested effects of acute systemic administration of β-Pro7-Ang III on renal hemodynamic and excretory function in anesthetized normotensive male and female rats. We also compared the natriuretic effects of acute intrarenal administration of native Ang III and β-Pro7-Ang III in the presence of systemic AT1R blockade in anesthetized female rats to allow for the differentiation of systemic versus direct intrarenal natriuretic actions of β-Pro7-Ang III. In both male and female rats, acute systemic administration of β-Pro7-Ang III elicited renal vasodilatation and natriuresis. Notably, greater renal vasodilatory effects were observed in female versus male rats at the highest dose of β-Pro7-Ang III administered. Moreover, intra-renal administration of β-Pro7-Ang III produced significant natriuretic effects in female rats and, like Ang III, evoked AT2R translocation to the apical plasma membrane in renal proximal tubular cells. Taken together, our findings support the use of β-Pro7-Ang III as a novel AT2R agonist and experimental tool for exploring AT2R function and its potential as a therapeutic target. Furthermore, our findings provide further evidence of a sex-specific influence of AT2R stimulation on renal function.
Cortical cells are altered by factors including bone morphogenetic protein released from a placental barrier model under altered oxygenation
Authors: Leinster Veronica H.L. , Phillips Thomas J. , Jones Nicola , Sanderson Sharon , Simon Katja , Hanley Jon , Case Charles Patrick 
Published: March 2020

Abstract
Episodes of hypoxia and hypoxia/reoxygenation during foetal development have been associated with increased risk of neurodevelopmental conditions presenting in later life.
The mechanism for this is not understood; however, several authors have suggested that the placenta plays an important role. Previously we found both placentas from a maternal hypoxia model and pre-eclamptic placentas from patients release factors lead to a loss of dendrite complexity in rodent neurons. Here to further explore the nature and origin of these secretions we exposed a simple in vitro model of the placental barrier, consisting of a barrier of human cytotrophoblasts, to hypoxia or hypoxia/reoxygenation. We then exposed cortical cultures from embryonic rat brains to the conditioned media (CM) from below these exposed barriers and examined changes in cell morphology, number, and receptor presentation.
The barriers released factors that reduced dendrite and astrocyte process lengths, decreased GABAB1 staining, and increased astrocyte number. The changes in astrocytes required the presence of neurons and were prevented by inhibition of the SMAD pathway and by neutralising Bone Morphogenetic Proteins (BMPs) 2/4. Barriers exposed to hypoxia/reoxygenation also released factors that reduced dendrite lengths but increased GABAB1 staining. Both oxygen changes caused barriers to release factors that decreased GluN1, GABAAα1 staining and increased GluN3a staining. We find that hypoxia in particular will elicit the release of factors that increase astrocyte number and decrease process length as well as causing changes in the intensity of glutamate and GABA receptor staining. There is some evidence that BMPs are released and contribute to these changes.
Oral and Oropharyngeal Sensory Function in Adults With Chronic Obstructive Pulmonary Disease
Authors: da Rosa Fernanda Borowsky , Pasqualoto Adriane Schmidt , Steele Catriona M. , Mancopes Renata 
Published: March 2020

Introduction
The oral cavity and pharynx have a rich sensory system composed of specialized receptors. The integrity of oropharyngeal sensation is thought to be fundamental for safe and efficient swallowing. Chronic obstructive pulmonary disease (COPD) patients are at risk for oropharyngeal sensory impairment due to frequent use of inhaled medications and comorbidities including gastroesophageal reflux disease.

Objective
This study aimed to describe and compare oral and oropharyngeal sensory function measured using noninstrumental clinical methods in adults with COPD and healthy controls.

Method

Participants included 27 adults (18 men, nine women) with a diagnosis of COPD and a mean age of 66.56 years (
SD
= 8.68). The control group comprised 11 healthy adults (five men, six women) with a mean age of 60.09 years (
SD
= 11.57). Spirometry measures confirmed reduced functional expiratory volumes (% predicted) in the COPD patients compared to the control participants. All participants completed a case history interview and underwent clinical evaluation of oral and oropharyngeal sensation by a speech-language pathologist. The sensory evaluation explored the detection of tactile and temperature stimuli delivered by cotton swab to six locations in the oral cavity and two in the oropharynx as well as identification of the taste of stimuli administered in 5-ml boluses to the mouth. Analyses explored the frequencies of accurate responses regarding stimulus location, temperature and taste between groups, and between age groups (“≤ 65 years” and “> 65 years”) within the COPD cohort.

Results

We found significantly higher frequencies of reported use of inhaled medications (
p
< .001) and xerostomia (
p
= .003) in the COPD cohort. Oral cavity thermal sensation (
p
= .009) was reduced in the COPD participants, and a significant age-related decline in gustatory sensation was found in the COPD group (
p
= .018).

Conclusion
This study found that most of the measures of oral and oropharyngeal sensation remained intact in the COPD group. Oral thermal sensation was impaired in individuals with COPD, and reduced gustatory sensation was observed in the older COPD participants. Possible links between these results and the use of inhaled medication by individuals with COPD are discussed.
Implications of Compression Race Suit on Forced Vital Capacity: Assessment Considerations for Paradoxical Vocal Fold Motion in Competitive Female Swimmers
Authors: Sandage Mary J. , Dunn Lauren A. , Edwards Ryleigh , Pope Sara Ann 
Published: March 2020

PurposeThe purpose of this investigation was to quantify the extent to which forced vital capacity (FVC) in competitive swimmers may differ from nonswimmers and determine if compression race suits reduced FVC when compared to practice swimsuits. Identification of the differences in FVC between swimmers and nonswimmers as well as pulmonary function differences secondary to swimsuit construction may inform assessment of the competitive swimmer with paradoxical vocal fold motion (PVFM).MethodUsing a prospective, mixed within- and between-groups, repeated measures design with 10 female competitive swimmers and 13 female nonswimmers, FVC was measured and compared between the two groups. Further FVC assessment was completed with the swimmers to identify FVC differences between a practice suit and a compression racing suit.ResultsFVC in swimmers was significantly larger than FVC in nonswimmers by over 1 L. The predicted FVC volumes were significantly smaller than the actual FVC volumes for swimmers. No significant differences were identified between the practice swimsuit and the compression race suit or between the predicted and actual FVCs for the nonswimmer group.ConclusionsSwimmers have unique pulmonary function and physiology that require consideration during the assessment for PVFM to ascertain the extent to which the pulmonary system may be compromised from PVFM, reduced exercise intensity, or both. Knowledge of differential diagnoses and adequate characterization of pulmonary volumes in swimmers will improve assessment processes.
The subsurface habitability of small, icy exomoons
Authors: Tjoa J. N. K. Y. , Mueller M. , van der Tak F. F. S. 
Published: March 2020

Context. Assuming our Solar System as typical, exomoons may outnumber exoplanets. If their habitability fraction is similar, they would thus constitute the largest portion of habitable real estate in the Universe. Icy moons in our Solar System, such as Europa and Enceladus, have already been shown to possess liquid water, a prerequisite for life on Earth.
Aims. We intend to investigate under what thermal and orbital circumstances small, icy moons may sustain subsurface oceans and thus be “subsurface habitable”. We pay specific attention to tidal heating, which may keep a moon liquid far beyond the conservative habitable zone.
Methods. We made use of a phenomenological approach to tidal heating. We computed the orbit averaged flux from both stellar and planetary (both thermal and reflected stellar) illumination. We then calculated subsurface temperatures depending on illumination and thermal conduction to the surface through the ice shell and an insulating layer of regolith. We adopted a conduction only model, ignoring volcanism and ice shell convection as an outlet for internal heat. In doing so, we determined at which depth, if any, ice melts and a subsurface ocean forms.
Results. We find an analytical expression between the moon’s physical and orbital characteristics and the melting depth. Since this expression directly relates icy moon observables to the melting depth, it allows us to swiftly put an upper limit on the melting depth for any given moon. We reproduce the existence of Enceladus’ subsurface ocean; we also find that the two largest moons of Uranus (Titania and Oberon) could well sustain them. Our model predicts that Rhea does not have liquid water.
Conclusions. Habitable exomoon environments may be found across an exoplanetary system, largely irrespective of the distance to the host star. Small, icy subsurface habitable moons may exist anywhere beyond the snow line. This may, in future observations, expand the search area for extraterrestrial habitable environments beyond the circumstellar habitable zone.
Robustness of prediction for extreme adaptive optics systems under various observing conditions
Authors: van Kooten M. A. M. , Doelman N. , Kenworthy M. 
Published: March 2020

Context. For high-contrast imaging systems, such as VLT/SPHERE, the performance of the system at small angular separations is contaminated by the wind-driven halo in the science image. This halo is a result of the servo-lag error in the adaptive optics (AO) system due to the finite time between measuring the wavefront phase and applying the phase correction. One approach to mitigating the servo-lag error is predictive control.
Aims. We aim to estimate and understand the potential on-sky performance that linear data-driven prediction would provide for VLT/SPHERE under various turbulence conditions.
Methods. We used a linear minimum mean square error predictor and applied it to 27 different AO telemetry data sets from VLT/SPHERE taken over many nights under various turbulence conditions. We evaluated the performance of the predictor using residual wavefront phase variance as a performance metric.
Results. We show that prediction always results in a reduction in the temporal wavefront phase variance compared to the current VLT/SPHERE AO performance. We find an average improvement factor of 5.1 in phase variance for prediction compared to the VLT/SPHERE residuals. When comparing to an idealised VLT/SPHERE, we find an improvement factor of 2.0. Under our 27 different cases, we find the predictor results in a smaller spread of the residual temporal phase variance. Finally, we show there is no benefit to including spatial information in the predictor in contrast to what might have been expected from the frozen flow hypothesis. A purely temporal predictor is best suited for AO on VLT/SPHERE.
Conclusions. Linear prediction leads to a significant reduction in phase variance for VLT/SPHERE under a variety of observing conditions and reduces the servo-lag error. Furthermore, prediction improves the reliability of the AO system performance, making it less sensitive to different conditions.
International observational campaign of the 2014 eclipse of EE Cephei
Authors: Pieńkowski D. , Gałan C. , Tomov T. , Gazeas K. , Wychudzki P. , Mikołajewski M. , Kubicki D. , Staels B. , Zoła S. , Pakońska P. , Dȩbski B. , Kundera T. , Ogłoza W. , Dróżdż M. , Baran A. , Winiarski M. , Siwak M. , Dimitrov D. , Kjurkchieva D. , Marchev D. , Armiński A. , Miller I. , Kołaczkowski Z. , Moździerski D. , Zahajkiewicz E. , Bruś P. , Pigulski A. , Smela T. , Conseil E. , Boyd D. , Conidis G. J. , Plauchu-Frayn I. , Heras T. A. , Kardasis E. , Biskupski M. , Kneip R. , Hambálek L. , Pribulla T. , Kundra E. , Garai Z. , Rodriguez D. , Kamiński T. , Dubois F. , Logie L. , Capetillo Blanco A. , Kankiewicz P. , Świerczyński E. , Martignoni M. , Sergey I. , Kare Trandem Qvam J. , Semkov E. , Ibryamov S. , Peneva S. , Gonzalez Carballo J.-L. , Ribeiro J. , Dean S. , Apostolovska G. , Donchev Z. , Corp L. , McDonald P. , Rodriguez M. , Sanchez A. , Wiersema K. , Conseil E. , Menke J. , Sergey I. , Richardson N. 
Published: March 2020

Context. EE Cep is one of few eclipsing binary systems with a dark, dusty disc around an invisible object similar to ε Aur. The system is characterised by grey and asymmetric eclipses every 5.6 yr that have significant variations in their photometric depth, ranging from ∼0.m5 to ∼2.m0.
Aims. The main aim of the observational campaign of the EE Cep eclipse in 2014 was to test the model of disc precession. We expected that this eclipse would be one of the deepest with a depth of ∼2.m0.
Methods. We collected multicoloured observations from almost 30 instruments located in Europe and North America. These photometric data cover 243 nights during and around the eclipse. We also analyzed low- and high-resolution spectra from several instruments.
Results. The eclipse was shallow with a depth of 0.m71 in the V band. The multicoloured photometry illustrates small colour changes during the eclipse with a total amplitude of order ∼+0.m15 in the B − I colour index. We updated the linear ephemeris for this system by including new times of minima, measured from the three most recent eclipses at epochs E = 9, 10, and 11. We acquired new spectroscopic observations, covering orbital phases around the eclipse, which were not observed in the past and increased the data sample, filling some gaps and giving better insight into the evolution of the Hα and Na I spectral line profiles during the primary eclipse.
Conclusions. The eclipse of EE Cep in 2014 was shallower than expected, measuring 0.m71 instead of ∼2.m0. This means that our model of disc precession needs revision.
Investigating the connection between γ-ray activity and the relativistic jet in 3C 273 during 2015−2019
Authors: Kim Dae-Won , Trippe Sascha , Kravchenko Evgeniya V. 
Published: March 2020

Context. The powerful radiation over the entire electromagnetic spectrum and its radio jet activity of the blazar 3C 273 offer the opportunity of studying the physics of γ-ray emission from active galactic nuclei. Since the historically strong outburst in 2009, 3C 273 showed relatively weak emission in the γ-ray band over several years. However, recent Fermi-Large Area Telescope observations indicate higher activity during 2015−2019.
Aims. We constrain the origin of the γ-ray outbursts toward 3C 273 and investigate their connection to the parsec-scale jet.
Methods. We generated Fermi-LAT γ-ray light curves with multiple binning intervals and studied the spectral properties of the γ-ray emission. Using a 3 mm ALMA light curve, we studied the correlation between radio and γ-ray emission. The relevant activity in the parsec-scale jet of 3C 273 was investigated with 7 mm VLBA observations that were obtained close in time to notable γ-ray outbursts.
Results. We find two prominent γ-ray outbursts in 2016 (MJD 57382) and 2017 (MJD 57883) accompanied by millimeter-wavelength flaring activity. The γ-ray photon index time series show a weak hump-like feature around the γ-ray outbursts. The monthly γ-ray flux–index plot indicates a transition from softer-when-brighter to harder-when-brighter states at 1.03 × 10−7 ph cm−2 s−1. A significant correlation between the γ-ray and millimeter-wavelength emission is found, and the radio lags the γ-rays by about 105−112 days. The 43 GHz jet images reveal the known stationary features (i.e., the core, S1, and S2) in a region upstream of the jet. We find an indication for a propagating disturbance and a polarized knot between the stationary components at about the times of the two γ-ray outbursts.
Conclusions. Our results support a parsec-scale origin for the observed higher γ-ray activity, which suggests that this is associated with standing shocks in the jet.