bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ozone autohemotherapy elevates PPAR-γ expression to lower blood lipid in treatment for psoriasis
Authors: Lu Jianyun , Ding Shu , Tang Zhen , Tong Xiaoliang , Dou Jianhua , Gao Lihua , Zeng Jinrong 
Published: March 2020

Abstract
Background: Psoriasis is widely accepted as a metabolic syndrome with significantly abnormal lipid metabolism and high blood lipids keep patients in a persistent low level of inflammatory condition. Hyperlipidemia and associated inflammatory reaction are believed to be the major risk factors contributing to the onset and recurrence of psoriasis. Peroxisome proliferator activated receptor-gamma (PPAR-γ) can effectively control the blood lipid level and inhibit inflammatory reaction. Methods: Ozone autohemotherapy (OAHT) was applied to treat psoriatic patients. The psoriasis area and severity index (PASI) score and blood lipid level were used to evaluate the efficacy. PPAR-γ expression level and correlation analysis were used to determine the OAHT target involving psoriasis. Results: We found that OAHT significantly decreased patients’ PASI scores and lipid blood level. Furthermore, we found that PPAR-γ expression in CD4+ T cells from patients with psoriasis was significantly lower than healthy controls, furtherly, we detected PPAR-γ expression upregulated after treatment compared with before treatment. There, we performed the correlation analysis on PPAR-γ level and patients’ PASI scores or lipid blood level. These results suggested OAHT might obviously improve patients’ PASI scores and decrease lipid blood level by regulating PPAR-γ expression. Conclusions: We found that OAHT can attenuate the condition of psoriatic patients and lower blood lipid by inducing PPAR-γ expression, suggesting that OAHT is effective in treating psoriasis and is worthy of further evaluations for its clinical applications.
Meningitis caused by Mycobacterium fortuitum mimicking tuberculous meningitis in an immunocompetent patient: a case report and literature review.
Authors: Lin Siran , Yang Qingluan , Wang Ting , Jia Wen , Chen Shu , Zhang Wenhong , shao lingyun 
Published: March 2020

Abstract
Background: Nontuberculous mycobacteria (NTM) are a large group of microorganisms that mainly affect persons who have immune deficiency. Mycobacterium fortuitum is one of them, which usually cause soft tissue or pulmonary disease. Meningitis caused by M. fortuitum is extremely rare and is often confused with tuberculous meningitis (TBM) due to its low prevalence. Case presentation: A 35-year-old woman was referred to our hospital with a history of headache, followed by fever, chills, cough, and vomiting. The results of mycobacterial culture and TSPOT. TB of her cerebrospinal fluid were positive. Therefore, she was diagnosed with tuberculous meningitis and was given anti-tuberculous chemotherapy. During the observation, the CSF results were not significantly improved. Finally, hsp65 gene PCR revealed that the real pathogen was M. fortuitum. The treatment therapy was changed and the patient recovered after one-year combined chemotherapy. Conclusions: In this case we described a patient with M. fortuitum meningitis who was immunocompetent with no trauma or surgical history. The wrong diagnosis with TBM was made due to positive results of both blood and CSF TSPOT. TB . The pathological evidence and microbiological analysis of hsp65 gene PCR suggested the real pathogen to be M. fortuitum . The diagnostic accuracy of blood and CSF TSPOT. TB in case of TBM is moderate. Infections of NTM cannot be excluded when the patient does not respond to anti-tuberculous therapy and it is of great significance to do molecular identification for clinical isolates of mycobacterium.
Does preoperative dipyridamole-thallium scanning reduce 90-day cardiac complications and mortality in patients with femoral neck fractures undergoing hemiarthroplasty?
Authors: Liao Chin-Yi , Tan Timothy L. , Lu Yu-Der , Wu Cheng-Ta , Lee Mel S , Kuo Feng-Chih 
Published: March 2020

Abstract
Background: This study aimed to evaluate the effect of dipyridamole-thallium scanning on 90-day cardiac complications and mortality in patients with femoral neck fracture treated with hemiarthroplasty. Methods : Between 2008 and 2015, 844 consecutive patients undergoing cemented or cementless hemiarthroplasty were identified from the database of a single level-one medical center. One-hundred and thirteen patients (13.4%) underwent the dipyridamole-thallium scan test prior to surgery, and 731 patients (86.6%) did not. Patient characteristics, comorbidities, surgical variables, and duration to surgery were recorded. Multivariate logistic regression was performed, and a propensity-score-matched cohort was identified in order to determine whether dipyridamole-thallium scanning was associated with 90-day cardiac complications and mortality. Results: The incidence of 90-day cardiac complications was 19.47% in the thallium scan group and 11.58% in the non-thallium scan group ( p = 0.343) among 452 patients after propensity score-matching. The 90-day mortality rate was similar in the two groups (0.88% vs. 1.18%, p = 0.795). In the propensity score-matched patients, utilization of the dipyridamole-thallium scan was not associated with cardiac complications (matched cohort, adjusted odds ratio [OR] = 1.32; 95% confidence interval [CI] 0.75 to 2.33) or mortality (adjusted OR = 0.75; 95% CI 0.08 to 6.71) within 90 days. Risk factors for cardiac complications were an American Society of Anesthesiologists grade 3 (OR 3.19, 95% CI 1.44 to 7.08) and pre-existing cardiac comorbidities (OR 5.56, 95% CI 3.35 to 9.25). Discussion : Preoperative dipyridamole-thallium scanning was not associated with reductions in 90-day cardiac complications and mortality in patients with femoral neck fracture undergoing hemiarthroplasty. The necessity for dipyridamole-thallium scanning should be re-evaluated in elderly patients with femoral neck fractures, given that this increases the duration to surgery.
Therapeutic effect and mechanisms of intra-articular injections of microRNA-140-5p on early-stage osteoarthritis in rats
Authors: Si Hai-bo , Yang Ti-min , Chen Yang , Mao Rui-wen , Wu Li-ming , Liang Ming-wei , Liu Shu-yun , Zeng Yi , Shen Bin 
Published: March 2020

Abstract
Background MicroRNAs (miRs) have received extensive attention in osteoarthritis (OA) pathogenesis in recent years, and our previous study have confirmed that single intra-articular injection (IAJ) of miR-140-5p alleviates early-stage OA (EOA) progression in rats. This study aims to further investigate the effects of single IAJ of miR-140-5p on different stage OA and multiple IAJs of miR-140-5p on EOA, as well as the potential mechanisms. Methods Firstly, OA model was surgically induced in rats, 9 rats were treated with IAJ of Cy5-miR-140-5p at 1 week after surgery, and fluorescence distribution was measured. Then, 72 rats were treated with single IAJ of miR-140-5p at different time after surgery or multiple IAJs of miR-140-5p at 1 week after surgery, and OA progression were evaluated macroscopically and histologically. Finally, bioinformatics analyses were performed and the potential targets and molecular mechanisms of miR-140-5p were predicted. Results Strong fluorescence was observed in the chondrocytes and joint where Cy5-miR-140-5p was injected. Behavioural scores, chondrocyte numbers and cartilage thickness in cartilage were higher, while pathological scores were lower in the miR-140-5p group than in the control group. Specifically, the earlier a single IAJ of miR-140-5p, the better the therapeutic effect, and multiple IAJs exhibited better therapeutic effect than single IAJ on EOA. Bioinformatics analyses predicted 84 potential target genes of rno-miR-140-5p and revealed that these genes enrich in various biological processes and pathways. Conclusions IAJs of miR-140-5p effectively alleviate EOA progression by modulating various biological processes and pathways, and may be a promising therapeutics for EOA.
Prevention of Mother-to-Child Transmission (PMTCT) of HIV in Iran: Situation Analysis of the Pilot Phase
Authors: Kazeroni Parvin Afsar , Gouya Mohammad Mehdi , Tira Mandana , Sargolzaiie Maryam , Eybpoosh Sana , Majdfar Zahra , Zareie Bushra , Ghaderi Ebrahim 
Published: March 2020

Abstract
Background The reproductive health and Prevention of Mother-to-Child Transmission of HIV (PMTCT) programs in Iran were integrated as a pilot project in September 2014.This study aims to provide a comprehensive evaluation and situation analysis of the PMTCT programme in Iran.Methods Pilot phase of PMTCT was launched in early September 2014 in selected centers including 170 health centers and 40 hospitals affiliated to medical universities of 16 provinces of Iran. In each medical university, a researcher-made checklist was administered to all newly-diagnosed HIV-positive pregnant women by the AIDS expert. Data was analyzed using SPSS 19 software.Results Overall, 69.4% of eligible pregnant women were enrolled in the pilot phase. From 134 reactive cases, 76 (56.7%) were confirmed as HIV positive. In 10 (13.2%) of HIV positive pregnant women, consumption of ARV was irregular. Also, 82.5% had CD4 count more than 350 after treatment with an average of 55.5% increase in the number of CD4 in comparison to the baseline, and 84.8% had viral load suppression (<200 copies/ml). Counseling and testing was done for the husbands of 75% of the women, and resulted in the identification of 15 (39.5%) new HIV cases among husbands. of tested individuals, 23 (60.5%) males already knew their HIV status and were registered HIV patients. HIV was diagnosed in one (1.5%) newborn.Conclusion Implementation of rapid HIV testing and PMTCT in Iran is strength in the national HIV control program. To eliminate MTCT, it is necessary to understand and overcome barriers and challenges the program faced in the pilot phase.
The Inhibition of H1N1 influenza virus-induced apoptosis by functionalized Selenium nanoparticles with β-Thujaplicin through ROS-mediated P53 and AKT signaling pathways
Authors: Wang Changbing , Zhao Mingqi , Lin Zhengfang , Guo Min , Xu Tiantian , Tang Ying , Chen Yi , Hua Liang , Zhong Jiayu , Lin Tao , Lian Guangwan , Chen Huanhui , Zhu Bing , Li Yinghua 
Published: March 2020

Abstract
β-Thujaplicin possess a variety of biological activities. The use of modified biological nanoparticles (NPs) to develop novel anti-influenza drugs has increased in recent years. Selenium nanoparticles (SeNPs) with antiviral has attracted increasing attention for biomedical intervention. Functionalized SeNPs by β-Thujaplicin (Se@TP) surface modified with superior antiviral were synthesized in this study. β-Thujaplicin decoration of SeNPs obviously inhibited H1N1 infection and were less toxicity. Se@TP could inhibit H1N1 from infecting Madin Darby Canine Kidney (MDCK) cells and block chromatin condensation and DNA fragmentation. Se@TP obviously prevented MDCK cells from generating reactive oxygen species (ROS). Furthermore, Se@TP prevent lung injury in H1N1 infected mice through eosin staining and hematoxylin in vivo . Additionally, when treated with Se@TP, the DNA damage of lung tissues reduced substantially by TUNEL-DAPI test. Mechanistic investigation revealed that Se@TP inhibited H1N1 influenza virus from infecting MDCK cells through induction of apoptosis via suppression AKT and p53 signaling pathways through Immunohistochemical assay. Our results suggest that β-Thujaplicin modified SeNPs as carriers is an efficient way to achieve antiviral pharmaceutical candidate for H1N1 influenza.
Lipid-polymer hybrid nanoparticles carrying doxorubicin and edelfosine combo for synergistic anticancer effect against drug-resistant Osteosarcoma
Authors: Yang Ping , Zhang Lian , Wang Tian , Liu Qi , Wang Jing , Wang Yaling , Tu Zhiquan , Lin Feng 
Published: March 2020

Abstract
In this study, we have prepared a novel folate receptor-targeted doxorubicin (DOX) and edelfosine (ET)-loaded lipid polymer hybrid nanoparticle to enhance the anticancer efficacy in osteosarcoma. The dual-drug loaded nanoparticles showed a nanosized morphology and physiological stability. The targeted nanoparticles showed enhanced cellular internalization and subcellular distribution in MG63 cancer cells compared to that of non-targeted nanoparticles. Among many ratios of DOX and ET, 1:1 ratiometric combinations of drugs were observed to be highly synergistic in killing the cancer cells. MTT assay and caspase-3/7 activity assay clearly showed the superior anticancer efficacy of DE-FPLN formulations in inducing the cancer cell death. In vitro results indicate that the co-administration of two drugs in a folic acid-targeted nanoparticle could potentially induce the apoptosis and cell death. In vivo results displayed the potency of tumor cell killing and significant suppression of tumor growth without any detectable side effects. The lipid polymer hybrid nanocarriers with multiple properties of high drug loading, sequential and ratiometric drug release, improved physiological stability, prolonged blood circulation, and tumor-specific targeting are promising for the delivery of multiple drugs in the treatment of osteosarcoma.
Smoking Cessation in Head and Neck Cancer Patients: A Mixed Methods Study
Authors: Smith Justin R , Woolley Torres , Brown Amy , Vangaveti Venkat , Chilkuri Madhavi 
Published: March 2020

Abstract
Background This study investigated the smoking behaviours and cessation rates of head and neck cancer patients and explored the barriers and facilitators to cessation. Methods A mixed methods sequential explanatory design was utilised. The quantitative data was collected through surveys prior to treatment commencement. The current smokers were followed up after treatment to determine their smoking status. One-on-one, semi-structured interviews were then conducted. Results A total of 64 participants were recruited. Participants who were current smokers were more likely to live in a rural location (p = 0.015), have lower education (p = 0.047), and report reduced social and family well-being (p = 0.005) when compared with those who were former or never smokers. The 7-day point prevalence cessation rate was 72% at 1-month follow-up and 67% at 3 months, while continuous smoking cessation was 54% at 1 month and 42% at 3 months. Participants who continued smoking were found to consume more alcohol (p = 0.032) and have higher psychological distress (p = 0.052). Qualitative analysis revealed 5 key themes associated with smoking cessation: the teachable moment of a cancer diagnosis and treatment, willpower and cessation aids, psychosocial environment, relationship with alcohol and marijuana, and health knowledge and beliefs surrounding smoking and cancer. Conclusion This study demonstrates that the majority of head and neck cancer patients are able to achieve smoking cessation, but relapses are common. Future cessation programs should be comprehensive, sustained and address co-morbid factors such as alcohol, marijuana and depression.
Circ_0002060 enhances doxorubicin resistance in osteosarcoma by regulating miR-198/ABCB1 axis
Authors: Liu Jun , Zhu Wenshuai , Ji Jianqin 
Published: March 2020

Abstract
Background Osteosarcoma (OS) is a common aggressive primary sarcoma of bone. Drug resistance is a huge obstacle to chemotherapy for cancer. This study aimed to investigate the role and mechanism of circ_0002060 in OS resistance to doxorubicin (DOX). Methods The levels of circ_0002060, miR-198 and ATP binding cassette subfamily B member 1 (ABCB1) were measured by quantitative real-time polymerase chain reaction or western blot assay. Kaplan-Meier analysis was performed to determine the relationship between circ_0002060 expression and overall survival. The half inhibition concentration (IC50) of doxorubicin was calculated by Cell Counting Kit-8 (CCK-8) assay. Cell proliferation was assessed by colony formation assay. Cell apoptosis was monitored by flow cytometry. The levels of apoptosis-related proteins were measured by western blot assay. Xenograft assay was utilized to analyze the effect of circ_0002060 on DOX resistance in vivo . The interaction among circ_0002060, miR-198 and ABCB1 were confirmed by dual-luciferase reporter assay, RNA immunoprecipitation assay or RNA pull-down assay. Results Circ_0002060 and ABCB1 were up-regulated, while miR-198 was down-regulated in OS tissues and DOX-resistant OS cells. Circ_0002060 silence reduced DOX resistance in vitro and in vivo . Moreover, circ_0002060 enhanced DOX resistance via sponging miR-198. Besides, miR-198 decreased DOX resistance by binding to ABCB1. In addition, circ_0002060 sponged miR-198 to up-regulate ABCB1 expression. Conclusion Circ_0002060 enhanced doxorubicin resistance of OS by regulating miR-198/ABCB1 axis, which provides potential therapeutic targets for OS therapy.
Avian leukosis virus subgroup J infection influencing composition of gut microbiota within chicken
Authors: Chen Yuan , Ni Jiajia , Li Hongwei 
Published: March 2020

Abstract
Background: Avian leukosis virus (ALV) is one of the major causes of disease in poultry. Probiotics play a critical role in animal health maintenance. Studies have indicated that viral infection can alter the composition of chicken gut flora. We hypothesized that the ALV-J infection could alter Probiotics composition in chicken fecal bacterial microbiome. To test is, we performed high-throughput 16S rRNA gene sequencing and evaluated gut flora proﬁles from the feces of ALV-J infected and healthy chickens. Results: Relative abundance at the phylum and species levels was calculated. The phylum Proteobacteria was expressed in higher abundance in ALV-J infected chickens than in healthy chickens. Additionally, the abundance of the opportunistic pathogen, Propionibacterium acnes, significantly increased in ALV-J infected chickens. Interestingly, ALV-J infection tended to be significantly decreased by the probiotics Lactobacillus helveticus and Lactobacillus reuteri. Conclusions: The study indicated ALV-J infection significantly altered the gut microbiota distribution in chickens. It also showed that ALV-J infection significantly influenced composition of the probiotics including Lactobacillus helveticus and Lactobacillus reuteri in chicken gut, which implied that to relieve avian leucosis subgroup J, microbiota-targeted therapies such as probiotic supplements are required.
TemporalNode2vec: Task-agnostic and Task-specific Temporal Node Embedding
Authors: HADDAD Mounir , BOTHOREL Cécile , LENCA Philippe , BEDART Dominique 
Published: March 2020

Abstract
The goal of graph embedding is to learn a representation of graphs vertices in a latent low-dimensional space in order to encode the structural information that lies in graphs. While real-world networks evolve over time, the majority of research focuses on static networks, ignoring local and global evolution patterns. A simplistic approach consists of learning nodes embeddings independently for each time step. This can cause unstable and inefficient representations over time.

In this paper, we present TemporalNode2vec, a novel dynamic graph embedding approach that learns continuous time-aware node representations. Overall, we demonstrate that our method improves node classification tasks comparing to previous static and dynamic approaches as it achieves up to 14% gain regarding the F1 score metric. We also prove that our model is more data-efficient than several baseline methods, as it affords to achieve good performances with a limited number of node representation features. Moreover, we develop and evaluate a task-specific variant of our method called TsTemporalNode2vec, aiming to improve the performances and the data-efficiency of node classification tasks.
Long-term Observation of Chinese Idiopathic Retinitis, Vasculitis, Aneurysms, and Neuroretinits (IRVAN) Patients and suggestion of a Revised Staging System
Authors: Qi Yuhe , Zhang Yongjin , Liu Wei , Li Lei , Ye Xiaofeng , Chang Qing , Wang Min , Xuan Yi , Guo Jingli , Xu Gezhi 
Published: March 2020

Abstract
Background To investigate the clinical characteristics and intervention effects on Chinese patients with idiopathic retinitis, vasculitis, aneurysms, and neuroretinitis (IRVAN).Methods The consecutive series patients received eye examinations including fundus fluorescein angiography (FFA), optical coherence tomography (OCT) and systemic testing. Laser, including pan-retinal photocoagulation (PRP), oral corticosteroid and Pars plana vitrectomy (PPV) were used during the investigation period.Results Forty-two eyes of 21 patients aged 15-58 years old, 19 females and 2 males, initial decimal BCVA NP ~1.5 (0.55±0.38), were included. Eighteen eyes reached Stage 2; 21 eyes with neovascularization of disc or elsewhere and/or vitreous hemorrhage (VH) reached Stage 3; one eye had neovascular glaucoma (NVG) at Stage 5. Two eyes had proliferative vitroretinopathy (PVR). Thirty-four eyes of 20 patients of stage 2 and 3 accepted retinal photocoagulation. 27 eyes completed PRP. PPV was performed for 3 eyes at the first visit due to VH or PVR. Three eyes developed to Stage 3 from Stage 2. One eye of Stage 3 developed to PDR with retinal detachment. Additional 5 eyes, 3 heavy VH, 1 PVR and 1 macular epiretinal membrane eye, received PPV during the follow-up. Intra-retinal microvascular abnormality (IRMA) was found in 7 eyes. Aneurysms on the optic nerve head and artery bifurcations disappeared in 8 eyes and decreased in number in 2 eyes after one year of photocoagulation. Seven eyes were found with BCVA ≤0.1, of which 3 experienced PVR, 2 exudative maculopathy, 1 acute macular neuroretinopathy (AMN) eye and 1 eye due to NVG. The BCVA of the last visit was NLP~1.2 (0.53±0.38), no significant difference was found with initial BCVA.Conclusions Female is more susceptible to IRVAN. IRMA and AMN are firstly described in IRVAN patients. PVR and exudative maculopathy, the major causes for severe visual impairment, are suggested in the revised staging system.