bluuebunny/crossref_metadata_2025_split huggingface dataset:
276 Effects of Hermetia illucens larvae meal on growth performance, carcass traits, and meat quality in finishing pigs
Authors: Ma Xianyong , Yu Miao , Li Zhenming , Deng Dun , Rong Ting 
Published: December 2019

Abstract
Object: This study aimed to investigate the effects of Hermetia illucens larvae meal on growth performance, carcass traits, and meat quality of finishing pigs. Methods: Seventy-two crossbred (Duroc × Landrace × Large White) pigs (76.0 ± 0.52 kg) were randomly allocated to three experimental treatments, and fed the diet with supplementation of 0%, 4%, or 8% Hermetia illucens larvae meal (HILM), respectively. The experiment lasted 42 days. Results: The average daily gain (ADG) of pigs in 4% HILM group was higher, and the feed gain ratio (F/G) was lower than the control and 8% HILM group (P &lt; 0.05). The loin-eye area, marbling scores, and inosine monophosphate (IMP) content of Longissimus thoracis muscle (LM) in 4% and 8% HILM group were higher than the control group (P &lt; 0.05). There were no diffenences on backfat thickness, pH value, meat color, drip loss among three groups. Compared with the control, 4% HILM significantly increased the content of intramuscular fat (IMF) (P &lt; 0.05). Meanwhile, the mRNA expression level of fatty acid synthase (FAS), acetyl CoA carboxylase α (ACCα), lipoprotein lipase (LPL), and MyHC-IIa in the LM was higher in 4% HILM group than that in 8% Hermetia illucens larvae meal and the control group (P &lt; 0.05). The abundance of Lactobacillus, Pseudobutyrivibrio, Roseburia, Oribacterium, and Faecalibacterium was higher in 4% Hermetia illucens larvae meal group than the control group (P &lt; 0.05); The abundance of Streptococcus was lower and Peptococcus was higher in 4% HILM group than 8% HILM group. Conclusion: HILM can be used as an effective feed ingredient in finishing pig’ diet up to 4% level, which was beneficial for the growth performance, carcass trait, and meat quality of pigs, and the underlying mechanism maybe due to the altered lipogenic potential, myofiber characteristics and the microbial composition regulated by HILM.
426 Awardee Talk: growing the quantity and quality of undergraduate research experiences in animal science
Authors: Jones Cassandra K 
Published: December 2019

Abstract
Undergraduate research is known to be one of experiential learning methods that helps animal science students gain critical thinking skills. There is high demand for these opportunities. For example, 77.9% of incoming freshmen in the Department of Animal Sciences & Industry at Kansas State University from 2016–2018 planned to conduct research sometime during their undergraduate career (225 of 289 students). Conventional 1:1 faculty:undergraduate mentoring methods in the Department were only serving 1.7% of the undergraduate population (21 of 1,212 students). This creates a unique challenge of increasing the number of undergraduate research opportunities, while maintaining the impact of individualized experiential learning. One method to address this challenge is the incorporation of a course-based research program. In this model, research projects are conducted during a conventional semester during scheduled classroom hours, with project components divided into three 5-week sections: 1) research preparation, including compliance requirements, hypothesis testing, experimental design, and protocol development; 2) data collection; and 3) data interpretation and dissemination. Students collect data as a team, but individually develop their own research abstract and poster to maintain a high level of experiential learning. By teaching multiples sections of this course per semester and incorporating the concepts into existing laboratories, 13.3% of students in the Department completed undergraduate research in the 2018–2019 academic year (159 of 1,197 students). To monitor the quality of these experiences, student critical thinking ability was assessed using the online Critical Thinking Basic Concepts & Understanding Test (Foundation for Critical Thinking, Tomales, CA). The type of research experience did not influence assessed skills (P &gt; 0.10). Thus, course-based undergraduate research experiences may be an option for growing the quantity and quality of undergraduate research experience in animal science.
126 Evaluation of true ileal amino acid digestibility of fermented soybean meal fed to nursery pigs
Authors: Jang Kibeom , Cheng Y C , Kim S W 
Published: December 2019

Abstract
Fermented soybean meal (FSBM) has smaller peptides and reduced anti-nutritional factors compared with those in conventional SBM. Apparent and true ileal digestibilities (AID and TID) of AA in FSBM were measured using both a direct method and a substitution method. Forty eight (24 barrows and 24 gilts) pigs at 7 kg BW were allotted to 4 dietary treatments (n = 12) based on a randomized complete block design with sex and initial body weight as blocks. Treatments includes NFD (semi-purified N free diet to measure endogenous AA loss), NFSB (NFD with 20% FSBM; Genebiotech Co., Ltd. and Nutraferma, Inc), CBD (corn basal diet), and CBSB (CBD at 80% and FSBM at 20%). NFD and NFSB were used to directly measure AID and TID AA of FSBM, whereas NFD, CBD, and CBSB were used to indirectly calculate AID and TID AA of FSBM using a substitution method. Experimental diets included TiO2 (0.4%). Pigs were fed experimental diets for 10 days. Feed allowance was based on 0.09 x BW0.75 kg per day. All pigs were euthanized at the end of the experiments to collect ileal digesta. Diets and ileal digesta were used to quantify TiO2 and AA. Data were analyzed using Mixed Procedure of SAS 9.4. Dietary treatment was the main effect and blocks were random effects. Endogenous AA loss was 2.48 g. When measured by a direct method, AID and TID of Lys, Met, Thr, and Trp were 72.1 and 89.8%, 74.9 and 92.1%, 61.0 and 88.5%, and 72.2 and 92.2%, respectively. When measured by a substitution method, AID and TID of Lys, Met, Thr, and Trp were 64.7 and 84.4%, 60.5 and 77.9%, 36.2 and 57.0%, and 58.2 and 81.0%, respectively. Overall, a direct method showed higher AID and TID AA in FSBM than those measured by a substitution method.
130 Comparison of amino acid digestibility in fish meal, porcine plasma, porcine red blood cell, and blood meal between broiler chickens and young pigs
Authors: Park Chan Sol , Naranjo Victor , Htoo John K , Adeola Olayiwola 
Published: December 2019

Abstract
The objective of this study was to compare the standardized ileal digestibility (SID) of AA in fish meal (FM), porcine plasma (PP), porcine red blood cell (RBC), and blood meal (BM) between broiler chickens and pigs. Five diets were prepared for both species including 4 diets containing test ingredients and a nitrogen-free diet. In Exp. 1, 416 male broiler chickens (initial BW = 705 ± 100.0 g) at d 18 post hatching were assigned to diets in a randomized complete block design with BW as a blocking factor. After 5 d of feeding, birds were euthanized for collection of ileal digesta. In Exp. 2, 10 barrows (initial BW = 22.1 ± 1.54 kg) surgically fitted with T-cannulas at the distal ileum were allotted to duplicate 5 × 4 incomplete Latin square design with 5 diets and 4 periods. Each experimental period consisted of 5-d adaptation and 2-d ileal digesta collection periods. Data for Exp. 1 and 2 were considered together as a 2 × 4 factorial arrangement with the effects of species (i.e., broiler chickens and pigs) and four diets (i.e., FM, PP, RBC, and BM). There were interactions (P &lt; 0.05) between species and diets on the SID of AA except for Pro. The SID of Lys, Met, Thr, and Trp in FM, PP, and RBC for broiler chickens were not different from values for pigs; however, the SID of respective AA in BM for broiler chickens were less than values for pigs. The SID of indispensable AA in BM were less than in FM, PP, or RBC within each species, but the SID of indispensable AA in BM for broiler chickens was less than values for pigs. In conclusion, utilization of AA in animal protein ingredients containing high concentration of CP varies between broiler chickens and pigs.
291 Effects of replacing inorganic trace minerals with low level of organic trace minerals on growth performance, tissue mineral deposition and mineral excretion in piglets
Authors: pei xun , liu lujie , wang geng , wang minqi , huai Minyan 
Published: December 2019

Abstract
This study was conducted to investigate effects of inorganic trace mineral (ITMs) replacement with low level organic trace minerals (glycine complex minerals, GCMs; proteolytic complex minerals, PCMs) on growth, tissue mineral retention and mineral excretion in piglets. A total of 128 weaned piglets (Duroc × Landrace × Largewhite, 14.37 ± 0.32kg BW) aged 40d were randomly divided into 4 dietary treatments and fed for 28 days, with 4 replicates of 8 piglets per treatment. Dietary treatments were:(T1) basal + ITMs (Cu, Zn, Fe and Mn, sulfate) at 100% commercial level;(T2) basal + ITMs at 50% commercial level + GCMs at 50% commercial level;(T3) basal + GCMs at 50% commercial level;(T4) basal + PCMs at 50% commercial level respectively. On day 29, six piglets from each group were slaughtered to collect serum, muscle and organ samples. The results showed that ADG of T1, T2 and T3 was higher than that of T4 (P &lt; 0.05), FCR of T1, T2 and T3 was lower than that of T4 (P &lt; 0.01). The retention of trace minerals in tissues and serum is significantly correlated with the supplemental level and source of trace minerals. When 50% commercial level ITMs replaced with GCMs, Cu in serum, heart, liver and pancreas, Fe in liver and muscle was increased (P &lt; 0.05), and liver Zn, Mn in heart, liver and muscle was increased (P &lt; 0.01) respectively. Muscle Zn in T3 was higher than that from T4 (P &lt; 0.05) with same level of organic minerals. Zn, Fe, Mn excreted from urine in pigs fed T2 diet was less than that from T1 (P &lt; 0.05), and urine Zn in T3 treatment is lower than that from T4 (P &lt; 0.05). This study indicated that dietary ITMs replacement with GCMs resulted in higher tissue minerals deposition, and organic trace minerals from GCMs exhibited higher bioavailability than those from PCMs.
295 Awardee Talk: Novel regulation of growth hormone by kisspeptin
Authors: Sartin James , Daniel Jay A , Foradori Chad , Whitlock Brian 
Published: December 2019

Abstract
Kisspeptin (KP) is critical regulator of reproductive function through it’s potent stimulation of gonadotropin releasing hormone (GnRH). In addition there has been inconclusive evidence to suggest intravenous KP can stimulate growth hormone (GH). Studies in 24 h fasted (but not fed) sheep determined that ICV injection of Kp-10 can increase plasma GH concentrations. This led to questions about the mechanism linking KP and GH. Since fasting is a critical requirement for the Kp effect on GH, studies were focused on factors that are linked to fasting within the hypothalamus and are known regulators of GH. Fasting causes a major upregulation of neuropeptide Y (NPY), a potent appetite regulator and in ruminants, a stimulator of GH. Pretreatment of BIBO 3304, an NPY receptor antagonist, blocked the effect of KP to increase GH and implicated NPY as a mediator of the KP effect. Indeed, KP injected ICV upregulated cFOS in NPY and GH Releasing Hormone (GHRH) cells in the arcuate nucleus and reduced cFOS in Somatostatin (SS) cells in the periventricular nucleus. Another factor, altered by fasting and capable of regulating GH, is Ghrelin. Both blockage of Ghrelin release and central blockade of Ghrelin receptors reduced or blocked release of GH after KP treatment. These experiments suggest an hypothesis that fasting upregulates central Ghrelin and NPY expression permitting the activation of NPY by KP. NPY in turn activates GHRH and inhibits SS to release GH.
374 Effects of maternal nutrition on secretion of leptin in the neonatal heifer and interaction of maternal and postnatal nutrition on age at puberty and postpubertal secretion of luteinizing hormone
Authors: O’Neil Meaghan M , West Sarah M , Maia Tatiane S , Cardoso Rodolfo C , Williams Gary L 
Published: December 2019

Abstract
Nutrition during gestation and early postnatally can program developmental changes in the offspring that persist until adult life. Here we tested the hypotheses that maternal nutritional during the second and third trimesters of gestation 1) affects neonatal secretion of leptin in heifer offspring, and 2) interacts with dietary energy intake during the juvenile period to affect age at puberty and postpubertal pulsatile secretion of luteinizing hormone (LH) in heifers. Bos indicus-influenced beef cows (n = 108) in 3 replicates bearing heifer fetuses were assigned randomly to receive low (L), moderate (M), or high energy (H) diets to achieve body condition scores (BCS) of 3–3.5 (thin; n = 36), 5.5–6 (moderate; n = 36), or 7.5–8 (obese; n = 36) by the second trimester of gestation until calving. Heifer offspring were weaned at 3–3.5 months of age and assigned randomly to either a high- (H; 1 kg/day) or low-gain (L; 0.5 kg/day) diet for 5 months. Blood samples from a subgroup of 30 heifers (Exp. 1) from cows in each maternal treatment (n = 10/treatment) were collected once every 2–3 days for 2 weeks after birth. In Exp. 2, 18 heifers representing 3 of the maternal × postnatal groups (HH, MH and LL) were ovariectomized and received estradiol replacement (OVX+E) after puberty. Pulsatile secretion of LH was evaluated for 5.5 hours. Maternal nutrition did not affect postnatal circulating leptin. Based on two replicates (n = 61), postnatal diets had the greatest effect on age at puberty (L &gt; H; P &lt; 0.001), with a lesser maternal diet effect (P &lt; 0.10), although LL &gt; HH by 99 days. None of the characteristics of pulsatile LH secretion differed among treatments in OVX + E heifers. Dietary effects on age at puberty were not associated with hormonal characteristics evaluated in these initial studies.
390 Effects of dietary-induced pH changes on feed digestion, nitrogen metabolism, fermentation, and biohydrogenation of polyunsaturated fatty acids in dual-flow continuous culture fermenters
Authors: Zeltwanger Joshua M , DiCostanzo Alfredo , Larson-Johnson Haley , Stern Marshall 
Published: December 2019

Abstract
Ruminal pH decreases as greater concentrations of grain are consumed, and as a result, reduces biohydrogenation of polyunsaturated fatty-acids. Our objective was to investigate effects of diet on pH and biohydrogenation activity in vitro. Eight dual-flow, continuous culture fermenters were blocked by side of the room and randomly assigned, within block, to 1 of 4 treatments. Treatments consisted of 100:0 roughage-to-concentrate (Grass), 50:50 roughage-to-concentrate plus flax oil supplement (Mix+F), 10:90 roughage-to-concentrate plus corn oil supplement (Con+C), or 10:90 roughage-to-concentrate plus flax oil supplement (Con+F). Fermenter pH, area under the curve, and maximum pH were lowest for Con+C and Con+F (P &lt; 0.05). Apparent and true DM, OM, and NDF digestibility were greater with grain inclusion (P &lt; 0.05) while ADF digestibility was not affected by treatment (P &gt; 0.10). Protein degradation was lowered as grain inclusion increased (P &lt; 0.05). Total VFA concentrations were lowest for Grass, highest for Con+C and Con+F, and intermediate for Mix+F (P &lt; 0.05). Acetate-to-propionate was lowest for Con treatments (P &lt; 0.05). Biohydrogenation of 18:2n-6 and 18:3n-3 fatty-acids were reduced (P &lt; 0.01) with inclusion of concentrate regardless of roughage-to-concentrate ratio. Concentrations of stearic acid recovered from effluent were greatest for Grass, intermediate for Mix+F, and lowest for both Con+C and Con+F (P &lt; 0.05). Linoleic acid proportions in effluent were greatest for Con+C and Con+F and intermediate for Mix+F (P &lt; 0.05), and inclusion of concentrate, regardless of amount, increased the amount linoleic acid recovered from fermenter overflow (P &lt; 0.01). Between Con+C and Con+F concentrations of alpha-linolenic acid (ALA) was greater when flax oil was supplemented (P &lt; 0.01). Proportions of ALA in effluent was similar (P &gt; 0.10) between Grass and Con+C; however, between Mix+F and Con+F ALA concentrations tended (P = 0.06) to be greater for Con+F. Omega6-to-omega3 ratios were similar across Grass, Mix+F, and Con+F (P &gt; 0.10) and greatest for Con+C (P &lt; 0.05).
396 Effect of zinc amino acid complexes on growth performance and carcass characteristics of finishing beef steers fed with or without ractopamine hydrochloride- a nine study pooled analysis
Authors: Reinhardt Christopher , Millican Allison , Wickersham Tryon , Larson Connie , Branine Mark 
Published: December 2019

Abstract
A nine-study retrospective pooled analysis was conducted to evaluate supplemental Zn provided as an amino acid complex (ZnAA) on growth performance, carcass quality and yield in finishing beef steers fed with or without ractopamine hydrochloride (RAC) prior to slaughter. Data from nine well-controlled studies conducted at university and commercial feedlot research facilities in AB, IA, KS, OK and TX were evaluated using 249 pens ranging from 6 to 275 steers /pen (Total number=14,096 animals.; initial BW = 340± 6.2 kg). Treatments evaluated were: (1) Control (CON) =basal diet with 0 to 11 mg-1·kg-1 DM ZnAA and no RAC; (2) RAC = 200 to 320 mg RAC ∙hd-1∙d-1 fed 28 to 42 d prior to slaughter; (3) ZnAA only =30 to 120 mg-1·kg-1 DM fed throughout finishing period; (4) ZnAA + RAC. Main effects of feeding RAC, ZnAA and RAC × ZnAA interaction were determined for growth performance and carcass data with pen as experimental unit and initial BW as covariate. Combined analyses indicated no RAC × ZnAA interactions (P ≥ 0.23). RAC main effect improved carcass-adjusted growth performance and increased HCW and ribeye area (P ≤ 0.01). Main effect ZnAA increased carcass-adjusted final BW, ADG, and HCW (P ≤ 0.01); improved carcass-adjusted Gain: DM feed ratio (P = 0.06), dressing percentage (P = 0.02), and increased calculated yield grade, backfat thickness and percentage of carcass internal fat (P ≤ 0.10). A RAC × ZnAA interaction (P ≤ 0.10) was observed for USDA quality and yield grade distribution of carcasses. Incidence and severity of liver abscesses were reduced (P ≤ 0.05) by feeding either ZnAA or RAC. Feeding ZnAA and RAC to finishing cattle separately or in combination were additive for producing incremental improvements in production and carcass traits of economic importance.
161 Preference for inorganic sources of calcium and phosphorus in sheep
Authors: Villalba Juan J , Pedernera Mariana , Mereu Alessandro 
Published: December 2019

Abstract
We determined whether lambs discriminate among mineral supplements containing P and Ca and if they modify their choices as a function of need. During 3 consecutive days, 40 lambs under a basal diet of alfalfa pellets and barley were offered 4-way choices among= (1) Bolifor® Monosodium phosphate (MSP-24% P), (2) Bolifor® Magnesium phosphate (MGP-15% P, 25% Mg), (3) Bolifor® Monocalcium phosphate (MCP-22.7% P, 16.5% Ca) and (4) Dicalcium phosphate (DCP-18.5%P, 23% Ca), and 2-way choices between DCP and each of the remaining minerals (Baseline). Subsequently, lambs were randomly assigned to four groups (10 lambs/group) and fed beet pulp-based basal rations such that the levels of Ca and P were low (LCa-LP), adequate (NCa-NP), low in Ca and adequate in P (LCa-NP), or adequate in Ca and low in P (NCa-LP). After 60 d, groups had the described 4- and 2-way choices (Post-deficiency). Intake and preference indexes during choice tests were analyzed as a split-plot design with lambs nested within group and day as the repeated measure. During Baseline, lambs ate and preferred MSP &gt; MGP &gt; DCP = MCP (P &lt; 0.0001). This pattern remained during post-deficiency, but in contrast to Baseline, lambs in NCa-LP, LCa-NP, and LCa-LP showed the greatest preferences for MSP (42%), MGP (42%) and MCP (22%), respectively (SEM = 4%; P &lt; 0.05). Groups under LCa in 2-way choices showed greater preference for MCP (56%) than groups under NCa (39%; SEM = 5%; P &lt; 0.05), and lambs in LCa-NP revealed the greatest preference for MGP (64%; SEM = 6%; P &lt; 0.05). Serum P concentration after post-deficiency choice tests (9.7 ± 0.7 mg/dL) returned to Baseline values, but Ca concentration was 0.9 mg/dL lower (9.3 ± 0.3 mg/dL; P &lt; 0.05) than in Baseline. Thus, lambs discriminated among mineral sources and displayed preferences reflecting their needs.
166 The effect of late lactation and pre-breeding nutrition on reproductive outcomes in an accelerated lambing system
Authors: Ehrhardt Richard A , Moody Jordan , Makela Barbara , Almudena Veiga-Lopez 
Published: December 2019

Abstract
Productivity and seasonal constraints in lamb production can be overcome with accelerated systems. Accelerated adoption, however, has been limited by seasonal constraints in reproduction. Preliminary data suggest that periconceptional nutrition affects reproduction in the sub-optimal, long day breeding season. To evaluate this, we randomly assigned prolific, multiparous, Polypay x Dorset ewes at mid-lactation to nutritional treatments (5) over 2 periods: last 30 days of lactation (LACT) followed by the flushing period (FLUSH) consisting of the 21-day pre-breeding and the 34-day breeding period (total 55 days). During LACT, ewes were fed 100% (C), 70% (Low), or 150% (High) of late lactation energy requirements according to lamb rearing status (single or multiple). At FLUSH, Low or High ewes were fed either 70% (Low) or 150% (High) of energy requirements for maintenance (C ewes fed at 100% maintenance during FLUSH). This design was repeated over two seasons: optimal (SHORT day, n = 117) and suboptimal (LONG day, n = 108) breeding periods at 42.73°N and 84.5°W. Ewes were exposed to Dorset rams in 5 pens at 4–5% rotated every 12h. Reproductive outcomes were examined by ultrasound (transabdominal for litter size and conception rate; transrectal for early pregnancy loss). GLM analysis revealed changes in bodyweight and body condition score (BCS) consistent between seasons (Table 1). Regardless of dietary treatment, litter size was higher in SHORT (P &lt; 0.05), while embryonic loss tended to be higher in LONG season (P = 0.08). Within season, treatment had no effect on reproductive outcomes, however prolonged undernutrition (Low-Low) reduced conception rate in LONG vs. SHORT season (100 vs.78%; P &lt; 0.05). These results indicate that 1) high conception rates in LONG are attainable with natural mating and 2) chronic undernutrition hampers conception during long day periods. Finally, traditional flushing protocols are inadequate to invoke an optimal ovulation response regardless of season in accelerated systems.