bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐Period Astronomical Forcing of Westerlies' Strength in Central Asia During Miocene Climate Cooling
Authors: Frisch Konstantin , Voigt Silke , Verestek Verena , Appel Erwin , Albert Richard , Gerdes Axel , Arndt Iris , Raddatz Jacek , Voigt Thomas , Weber Yuki , Batenburg Sietske J. 
Published: November 2019

AbstractThe continental expression of global cooling during the Miocene Climate Transition in Central Asia is poorly documented, as the tectonically active setting complicates the correlation of Neogene regional and global climatic developments. This study presents new geochemical data (CaSO4 content, carbonate δ13C and δ18O) from the endorheic alluvial‐lacustrine Aktau succession (Ili Basin, south‐east Kazakhstan) combined with findings from the previously published facies evolution. Time series analysis revealed long‐eccentricity forcing of the paleohydrology throughout the entire succession, split into several facies‐dependent segments. Orbital tuning, constrained by new laser ablation U‐Pb dates and a preexisting magnetostratigraphy, places the succession in a 5.0 Ma long interval in the middle to late Miocene (15.6 to 10.6 Ma). The long‐term water accumulation in the Ili Basin followed the timing of the Miocene Climate Transition, suggesting increased precipitation in the catchment area in response to climate cooling and stronger westerly winds. This was paced by minima of the 2.4 Ma eccentricity cycle, which favored the establishment of a discharge playa (~14.3 Ma) and a perennial lake (12.6 to 11.8 Ma). Furthermore, low obliquity amplitudes (nodes) caused a transient weakening of the westerlies at ~13.7 to 13.5 Ma and at ~12.7 Ma, resulting in negative hydrological budgets and salinization. Flooding of the windward Ili Basin coeval with aridification in the leeward basins suggests that the Tian Shan was a climate boundary already in the middle Miocene. Our results emphasize the impact of climate fluctuations on the westerlies' strength and thus on Central Asian hydrology.
Structural Evolution and Metasomatism of Subducted Metaophiolites in the Northwestern Alps
Authors: Tartarotti P. , Martin S. , Meyzen C. M. , Benciolini L. , Toffolo L. 
Published: November 2019

AbstractA subduction complex of the northwestern Alps consists of serpentinites, eclogitic metagabbros, flysch‐like metasediments, meta‐ophicarbonates, and gneissic slices. Unlike other subduction complexes, it contains unusual hybridized rocks described here for the first time in the northwestern Alps. They are preserved as patches interstitial in the metagabbro and as layers within metagabbros and serpentinites. The hybridized rocks are made of high modal zoisite/clinozoisite + white mica pseudomorphs of lawsonite, garnet, and amphibole associated with an Alpine eclogite‐facies fabric. While these eclogitic metagabbros are chemically comparable to oceanic oxide gabbros from the ultraslow Southwest Indian Ridge, the layers are extremely enriched in Al2O3 and CaO and depleted in TiO2, MgO, and SiO2 relative to metagabbros. Patches have a geochemical signature that is intermediate between that of layers and metagabbros. Trace element compositions of hybridized rocks suggest a contribution from a fluid derived from a mixed source made of sediments and serpentinites. Except for Ba, Rb, and K, layers are comparable to the global subducting sediments, indicating a sedimentary contribution, whereas the enrichment in Cr indicates a serpentinite contribution. Metasediment dehydration and chemical exchange of Ca and Sr have resulted in significant lawsonite crystallization in the subduction zone, as reflected by the ubiquitous presence of lawsonite pseudomorphs. In light of the unique textures and geochemical signature of the lawsonite pseudomorph‐bearing hybridized rocks, an origin by fluid‐rock interaction and Ca‐metasomatism in the subduction environment is inferred and considered in the Western Alps context.
Association of BMI category with change in children’s physical activity between ages 6 and 11 years: a longitudinal study
Authors: Jago Russell , Salway Ruth , Emm-Collison Lydia , Sebire Simon J. , Thompson Janice L. , Lawlor Deborah A. 
Published: November 2019

AbstractBackground/objectivesTo examine the association of body mass index (BMI) with change in children’s physical activity and sedentary time between ages 6 and 11.ParticipantsA total of 2132 children participated from 57 schools in Southwest England, from the B-PROACT1V study.MethodsMean minutes of MVPA and sedentary time per day were derived from accelerometer-based measurements at ages 6, 9 and 11. Linear multilevel models examined the association of BMI categories with MVPA and sedentary time between 6 and 11, adjusting for seasonality, wear time, gender and household education. Differences in change over time were examined using interaction terms.ResultsAverage weekday MVPA decreased between ages 6 and 11 by 2.2 min/day/year (95% CI: 1.9 to 2.5), with a steeper decline at weekends. Average sedentary time increased at a rate of 12.9 min/day/year (95% CI: 12.2 to 13.6). There were no differences in mean levels of MVPA by BMI categories at age 6, but differences emerged as children aged, with the gap between children who were healthy weight and overweight increasing by 1.7 min/day (95% CI: 0.8–2.6) every year, and between healthy and obese by 2.0 min/day (95% CI: 0.9–3.1) each year. Children who were overweight/obese engaged in less average weekday sedentary time at age 6 than those of healthy weight, but the gap closed by age 11.ConclusionMVPA declines and sedentary time increases on average for all children between ages 6 and 11. While there are no differences in activity levels by BMI category at age 6, differences in MVPA emerge over time for those who are overweight and obese. Developing interventions that support children to retain activity levels as they approach older childhood, particularly those who are overweight/obese could improve public health.
A split-GFP tool reveals differences in the sub-mitochondrial distribution of wt and mutant alpha-synuclein
Authors: Vicario Mattia , Cieri Domenico , Vallese Francesca , Catoni Cristina , Barazzuol Lucia , Berto Paola , Grinzato Alessandro , Barbieri Laura , Brini Marisa , Calì Tito 
Published: November 2019

AbstractParkinson’s disease (PD), the second most common neurodegenerative disorder, is characterized by dopaminergic neuronal loss that initiates in the substantia nigra pars compacta and by the formation of intracellular inclusions mainly constituted by aberrant α-synuclein (α-syn) deposits known as Lewy bodies. Most cases of PD are sporadic, but about 10% are familial, among them those caused by mutations in SNCA gene have an autosomal dominant transmission. SNCA encodes α-syn, a small 140-amino acids protein that, under physiological conditions, is mainly localized at the presynaptic terminals. It is prevalently cytosolic, but its presence has been reported in the nucleus, in the mitochondria and, more recently, in the mitochondria-associated ER membranes (MAMs). Whether different cellular localizations may reflect specific α-syn activities is presently unclear and its action at mitochondrial level is still a matter of debate. Mounting evidence supports a role for α-syn in several mitochondria-derived activities, among which maintenance of mitochondrial morphology and modulation of complex I and ATP synthase activity. α-syn has been proposed to localize at the outer membrane (OMM), in the intermembrane space (IMS), at the inner membrane (IMM) and in the mitochondrial matrix, but a clear and comparative analysis of the sub-mitochondrial localization of WT and mutant α-syn is missing. Furthermore, the reasons for this spread sub-mitochondrial localization under physiological and pathological circumstances remain elusive. In this context, we decided to selectively monitor the sub-mitochondrial distribution of the WT and PD-related α-syn mutants A53T and A30P by taking advantage from a bimolecular fluorescence complementation (BiFC) approach. We also investigated whether cell stress could trigger α-syn translocation within the different mitochondrial sub-compartments and whether PD-related mutations could impinge on it. Interestingly, the artificial targeting of α-syn WT (but not of the mutants) to the mitochondrial matrix impacts on ATP production, suggesting a potential role within this compartment.
High-glucose-induced miR-214-3p inhibits BMSCs osteogenic differentiation in type 1 diabetes mellitus
Authors: Wang Rongze , Zhang Yuanxu , Jin Fujun , Li Gongchen , Sun Yao , Wang Xiaogang 
Published: November 2019

AbstractType 1 diabetes mellitus (T1DM) is an autoimmune insulin-dependent disease associated with destructive bone homeostasis. Accumulating evidence has proven that miRNAs are widely involved in the regulation of bone homeostasis. However, whether miRNAs also regulate osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in T1DM mice is under exploration. In this study, miRNA microarray was utilized to screen the differentially expressed miRNAs, which uncovered that miR-214-3p potentially inhibited BMSCs osteogenic differentiation in T1DM mice. We found that high glucose suppressed BMSCs osteogenic differentiation with significant elevation of the miR-214-3p expression. Further study found that the osteogenic differentiation of BMSCs was inhibited by AgomiR-214-3p while enhanced by AntagomiR-214-3p in BMSCs supplemented with high glucose. Moreover, we found that miR-214-3p knockout T1DM mice were resistant to high-glucose-induced bone loss. These results provide a novel insight into an inhibitory role of high-glucose-induced miR-214-3p in BMSCs osteogenic differentiation both in vitro and in vivo. Molecular studies revealed that miR-214-3p inhibits BMSCs osteogenic differentiation by targeting the 3′-UTR of β-catenin, which was further corroborated in human bone specimens and BMSCs of T1DM patients. Taken together, our study discovered that miR-214-3p is a pivotal regulator of BMSCs osteogenic differentiation in T1DM mice. Our findings also suggest that miR-214-3p could be a potential target in the treatment of bone disorders in patients with T1DM.
Diffractive small angle X-ray scattering imaging for anisotropic structures
Authors: Kagias Matias , Wang Zhentian , Birkbak Mie Elholm , Lauridsen Erik , Abis Matteo , Lovric Goran , Jefimovs Konstantins , Stampanoni Marco 
Published: November 2019

AbstractInsights into the micro- and nano-architecture of materials is crucial for understanding and predicting their macroscopic behaviour. In particular, for emerging applications such as meta-materials, the micrometer scale becomes highly relevant. The micro-architecture of such materials can be tailored to exhibit specific mechanical, optical or electromagnetic behaviours. Consequently, quality control at micrometer scale must be guaranteed over extended areas. Mesoscale investigations over millimetre sized areas can be performed by scanning small angle X-ray scattering methods (SAXS). However, due to their long measurement times, real time or operando investigations are hindered. Here we present a method based on X-ray diffractive optics that enables the acquisition of SAXS signals in a single shot (few milliseconds) over extended areas. This method is applicable to a wide range of X-ray sources with varying levels of spatial coherence and monochromaticity, as demonstrated from the experimental results. This enables a scalable solution of spatially resolved SAXS.
ZEB1/NuRD complex suppresses TBC1D2b to stimulate E-cadherin internalization and promote metastasis in lung cancer
Authors: Manshouri Roxsan , Coyaud Etienne , Kundu Samrat T. , Peng David H. , Stratton Sabrina A. , Alton Kendra , Bajaj Rakhee , Fradette Jared J. , Minelli Rosalba , Peoples Michael D. , Carugo Alessandro , Chen Fengju , Bristow Christopher , Kovacs Jeffrey J. , Barton Michelle C. , Heffernan Tim , Creighton Chad J. , Raught Brian , Gibbons Don L. 
Published: November 2019

AbstractNon-small cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide, due in part to the propensity of lung cancer to metastasize. Aberrant epithelial-to-mesenchymal transition (EMT) is a proposed model for the initiation of metastasis. During EMT cell-cell adhesion is reduced allowing cells to dissociate and invade. Of the EMT-associated transcription factors, ZEB1 uniquely promotes NSCLC disease progression. Here we apply two independent screens, BioID and an Epigenome shRNA dropout screen, to define ZEB1 interactors that are critical to metastatic NSCLC. We identify the NuRD complex as a ZEB1 co-repressor and the Rab22 GTPase-activating protein TBC1D2b as a ZEB1/NuRD complex target. We find that TBC1D2b suppresses E-cadherin internalization, thus hindering cancer cell invasion and metastasis.
Coevolution of male and female mate choice can destabilize reproductive isolation
Authors: Aubier Thomas G. , Kokko Hanna , Joron Mathieu 
Published: November 2019

AbstractSexual interactions play an important role in the evolution of reproductive isolation, with important consequences for speciation. Theoretical studies have focused on the evolution of mate preferences in each sex separately. However, mounting empirical evidence suggests that premating isolation often involves mutual mate choice. Here, using a population genetic model, we investigate how female and male mate choice coevolve under a phenotype matching rule and how this affects reproductive isolation. We show that the evolution of female preferences increases the mating success of males with reciprocal preferences, favouring mutual mate choice. However, the evolution of male preferences weakens indirect selection on female preferences and, with weak genetic drift, the coevolution of female and male mate choice leads to periodic episodes of random mating with increased hybridization (deterministic ‘preference cycling’ triggered by stochasticity). Thus, counterintuitively, the process of establishing premating isolation proves rather fragile if both male and female mate choice contribute to assortative mating.
Vapour-phase-transport rearrangement technique for the synthesis of new zeolites
Authors: Kasneryk Valeryia , Shamzhy Mariya , Zhou Jingtian , Yue Qiudi , Mazur Michal , Mayoral Alvaro , Luo Zhenlin , Morris Russell E. , Čejka Jiří , Opanasenko Maksym 
Published: November 2019

AbstractOwing to the significant difference in the numbers of simulated and experimentally feasible zeolite structures, several alternative strategies have been developed for zeolite synthesis. Despite their rationality and originality, most of these techniques are based on trial-and-error, which makes it difficult to predict the structure of new materials. Assembly-Disassembly-Organization-Reassembly (ADOR) method overcoming this limitation was successfully applied to a limited number of structures with relatively stable crystalline layers (UTL, UOV, *CTH). Here, we report a straightforward, vapour-phase-transport strategy for the transformation of IWW zeolite with low-density silica layers connected by labile Ge-rich units into material with new topology. In situ XRD and XANES studies on the mechanism of IWW rearrangement reveal an unusual structural distortion-reconstruction of the framework throughout the process. Therefore, our findings provide a step forward towards engineering nanoporous materials and increasing the number of zeolites available for future applications.
Community-level respiration of prokaryotic microbes may rise with global warming
Authors: Smith Thomas P. , Thomas Thomas J. H. , García-Carreras Bernardo , Sal Sofía , Yvon-Durocher Gabriel , Bell Thomas , Pawar Samrāt 
Published: November 2019

AbstractUnderstanding how the metabolic rates of prokaryotes respond to temperature is fundamental to our understanding of how ecosystem functioning will be altered by climate change, as these micro-organisms are major contributors to global carbon efflux. Ecological metabolic theory suggests that species living at higher temperatures evolve higher growth rates than those in cooler niches due to thermodynamic constraints. Here, using a global prokaryotic dataset, we find that maximal growth rate at thermal optimum increases with temperature for mesophiles (temperature optima $$\lesssim 4{5}\ ^{\circ }$$
≲
4

5

∘

C), but not thermophiles ($$\gtrsim 4{5}\ ^{\circ }$$
≳
4

5

∘

C). Furthermore, short-term (within-day) thermal responses of prokaryotic metabolic rates are typically more sensitive to warming than those of eukaryotes. Because climatic warming will mostly impact ecosystems in the mesophilic temperature range, we conclude that as microbial communities adapt to higher temperatures, their metabolic rates and therefore, biomass-specific CO$${}_{2}$$

2

production, will inevitably rise. Using a mathematical model, we illustrate the potential global impacts of these findings.
Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs
Authors: Grozhik Anya V. , Olarerin-George Anthony O. , Sindelar Miriam , Li Xing , Gross Steven S. , Jaffrey Samie R. 
Published: November 2019

AbstractN1-methyladenosine (m1A) was proposed to be a highly prevalent modification in mRNA 5’UTRs based on mapping studies using an m1A-binding antibody. We developed a bioinformatic approach to discover m1A and other modifications in mRNA throughout the transcriptome by analyzing preexisting ultra-deep RNA-Seq data for modification-induced misincorporations. Using this approach, we detected appreciable levels of m1A only in one mRNA: the mitochondrial MT-ND5 transcript. As an alternative approach, we also developed an antibody-based m1A-mapping approach to detect m1A at single-nucleotide resolution, and confirmed that the commonly used m1A antibody maps sites to the transcription-start site in mRNA 5’UTRs. However, further analysis revealed that these were false-positives caused by binding of the antibody to the m7G-cap. A different m1A antibody that lacks cap-binding cross-reactivity does not show enriched binding in 5’UTRs. These results demonstrate that high-stoichiometry m1A sites are exceedingly rare in mRNAs and that previous mappings of m1A to 5’UTRs were the result of antibody cross-reactivity to the 5’ cap.
Adjacent cationic–aromatic sequences yield strong electrostatic adhesion of hydrogels in seawater
Authors: Fan Hailong , Wang Jiahui , Tao Zhen , Huang Junchao , Rao Ping , Kurokawa Takayuki , Gong Jian Ping 
Published: November 2019

AbstractElectrostatic interaction is strong but usually diminishes in high ionic-strength environments. Biosystems can use this interaction through adjacent cationic–aromatic amino acids sequence of proteins even in a saline medium. Application of such specific sequence to the development of cationic polymer materials adhesive to negatively charged surfaces in saline environments is challenging due to the difficulty in controlling the copolymer sequences. Here, we discover that copolymers with adjacent cation–aromatic sequences can be synthesized through cation–π complex-aided free-radical polymerization. Sequence controlled hydrogels from diverse cation/aromatic monomers exhibit fast, strong but reversible adhesion to negatively charged surfaces in seawater. Aromatics on copolymers are found to enhance the electrostatic interactions of their adjacent cationic residues to the counter surfaces, even in a high ionic-strength medium that screens the electrostatic interaction for common polyelectrolytes. This work opens a pathway to develop adhesives using saline water.
A study on the effect of fingerprints in a wet system
Authors: Kim Donghyun , Yun Dongwon 
Published: November 2019

AbstractIn this paper, we study the influence of the fingerprint and sweat on the fingerprint on the friction between the hand and an object. When sweat contacts a finger or an object, it is sometimes easy to pick up the object. In particular, we can see this phenomenon when grasping a thin object such as paper and vinyl. The reason for this phenomenon is the increase of friction force, and this paper physically analyzes this natural phenomenon. To this end, we investigate the cause of the friction force between a solid and liquid to calculate the friction force when water is present within the fingerprint. To support the theoretical analysis, we conduct experiments to measure the friction force by making a finger-shaped silicon specimen. By comparing the theoretical and experimental results, we defined the change of friction force if there was water in the fingerprint. Through this study, it is possible to analyze the role of the fingerprint and sweat on the finger, and thereby explain the friction change depending on the amount of sweat.
Synergistic PA and HA mutations confer mouse adaptation of a contemporary A/H3N2 influenza virus
Authors: Baz Mariana , M’hamdi Zeineb , Carbonneau Julie , Lavigne Sophie , Couture Christian , Abed Yacine , Boivin Guy 
Published: November 2019

AbstractThe mouse is the most widely used animal model for influenza virus research. However, the susceptibility of mice to seasonal influenza virus depends on the strain of mouse and on the strain of the influenza virus. Seasonal A/H3N2 influenza viruses do not replicate well in mice and therefore they need to be adapted to this animal model. In this study, we generated a mouse-adapted A/H3N2 virus (A/Switzerland/9715293/2013 [MA-H3N2]) by serial passaging in mouse lungs that exhibited greater virulence compared to the wild-type virus (P0-H3N2). Seven mutations were found in the genome of MA-H3N2: PA(K615E), NP(G384R), NA(G320E) and HA(N122D, N144E, N246K, and A304T). Using reverse genetics, two synergistically acting genes were found as determinants of the pathogenicity in mice. First, the HA substitutions were shown to enhanced viral replication in vitro and, second, the PA-K615E substitution increased polymerase activity, although did not alter virus replication in vitro or in mice. Notably, single mutations had only limited effects on virulence in vitro. In conclusion, a co-contribution of HA and PA mutations resulted in a lethal mouse model of seasonal A/H3N2 virus. Such adapted virus is an excellent tool for evaluation of novel drugs or vaccines and for study of influenza pathogenesis.
First nearly complete skull of Gallotia auaritae (lower-middle Pleistocene, Squamata, Gallotiinae) and a morphological phylogenetic analysis of the genus Gallotia
Authors: Cruzado-Caballero Penélope , Castillo Ruiz Carolina , Bolet Arnau , Colmenero Juan Ramón , De la Nuez Julio , Casillas Ramón , Llacer Sergio , Bernardini Federico , Fortuny Josep 
Published: November 2019

AbstractThe Canary Islands are an Atlantic archipelago known for its high number of endemic species. Among the most known endemic vertebrate species are the giant lizards of the genus Gallotia. We describe the cranial osteology of the first almost complete and articulated fossil skull of the taxon Gallotia auaritae, recovered from the lower-middle Pleistocene of the La Palma island. In this work, X-ray computed microtomography images were used to perform an exhaustive phylogenetic analysis where most of the extant and fossil species of the genus Gallotia were included for first time. This analysis recovered a monophyletic Gallotia clade with similar topology to that of molecular analyses. The newly described specimen shares some characters with the group formed by G. bravoana, G. intermedia and G. simonyi, G. auaritae, and its position is compatible with a referral to the latter. Our study adds new important data to the poorly known cranial morphology of G. auaritae, and the phylogenetic analysis reveals an unexpected power of resolution to obtain a morphology-based phylogeny for the genus Gallotia, for inferring the phylogenetic position of extinct species and for helping in the identification of fossil specimens.