bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengembangan Mobile Learning IPA (MLI) Sasirangan Materi Pencemaran Lingkungan untuk Peserta Didik SMP
Authors: Amrita Putri Diana , Kuswanto Heru 
Published: October 2020

This study is aimed at: (1) creating Sasirangan Science Mobile (MLI Sasirangan) Learning product for junior high school students and (2) determining the feasibility level of MLI Sasirangan according to experts, practitioners, academics, and the response of students in limited trial test as users. The type of this research is research and development (R&D). Furthermore, the result of this research shows that: (1) Android-web-application-based MLI Sasirangan was created from Learning Management System (LMS) Moodle. The material was delivered by integrating the waste of typical South Kalimantan fabric making with the environmental pollution material and learned online; (2) based on the assessment result from media and material experts, this product obtained a value of 4.73 and 4.48 with a feasibility percentage of 94.6% and 89.56%. Meanwhile, the evaluation result from practitioners and peers, the values obtained were 4.45 and 4.64. Moreover, from the limited trial test, this product obtained a value of 4.47 with the percentage of feasibility is of 89.00%, 92.80% and 89.40% respectively. Therefore, it can be concluded that MLI Sasirangan which has been developed is considered feasible with very good criteria to be used as science learning media.
Keywords: Mobile learning, android, sasirangan, science.
Mastering Internal Growth and Transformation after Miscarriage experience: A Qualitative Study
Authors: Shin Gisoo , Kim Hye Jin , Kim Sung Hae 
Published: October 2020

Abstract
Background: The loss is common among many people, but not everyone goes through all of the stages or experiences. Miscarriage is a unique loss experience among the women. The loss and grief experienced after a miscarriage may bring both losses and gains in life. The purpose of this research was to identify the experiences of South Korean women after miscarriage.Methods: This was a qualitative study based on the Strauss and Corbin methods. A total of 23 participants who had all experienced more than three miscarriages participated in this study. The data were collected through in-depth interviews conducted from May to July 2017.Results: We found that 47 sub-categories, and 15 categories through the open coding process. Six properties of mastering internal growth and transformation following a miscarriage were identified. “The experience internal growth and transformation through the loss and mother fetal attachment” was proposed as core category. Conclusions: The findings of this study were meaningful in that they revealed the process of women experiencing internal growth and transformation after miscarriage, breaking away from focusing on negative feelings related to miscarriage. We presented a different angle of experiencing internal growth and transformation with a new perspective on life. The findings indicated the valuable support in the enhanced recovery education and might help them understand the changes in their lives after miscarriage, away from the traditional view of women who have experienced miscarriage.
Magnetic Resonance Imaging Evaluation of Tendon Integrity Initially After Arthroscopic Rotator Cuff Repair: Single Row Versus Suture Bridge Technique
Authors: Dai Zhangsheng , Li Hong , Chen Shiyi 
Published: October 2020

Abstract
Background: Previous studies have shown re-tear signs of rotator cuff tendon tearing after single row or suture bridge repair during follow-up. Few studies have investigated tendon integrity initially after successful rotator cuff repair. The purpose of this study was to assess and compare the magnetic resonance imaging (MRI) appearance of the completely repaired rotator cuff initially after surgery between single row and suture bridge techniques.Methods: Fifty patients with full-thickness rotator cuff tears were included in this study. Twenty-five patients underwent arthroscopic rotator cuff repair with single row technique (SR group) or and the other 25 patients underwent double row suture bridge technique (SB group). MRI examinations were performed at the second day after arthroscopic rotator cuff repair. Postoperative rotator cuff integrity was classified into 5 categories according to Sugaya method.Results: Postoperative MRI revealed 3 type I, 9 type II, 8 type III, 5 type IV, and 0 type V in single-row group (SR group), and 1 type I, 11 type II, 10 type III, 3 type IV, and no type V in suture bridge group (SB group). The postoperative re-tear rate was 20% in SR group and 12% in SB group, while no significant difference of re-tear rate was detected between groups (p = 0.70). In the 3 type IV cases of SB group, 1 case was found to have configuration deformity, and the other 2 cases revealed poor tissue quality of rotator cuff under arthroscopy. In the 5 type IV cases of SR group, 3 cases were found to have configuration deformity, and the other 2 cases revealed poor tissue quality of rotator cuff under arthroscopy.Conclusion: No difference of tendon integrity was detected between single row and suture bridge techniques initially after surgery. The re-tear sign of successfully repaired rotator cuff tendon was related with poor tendon tissue quality and also with configuration deformity after surgery.
Effects of Oral Contraceptives and Metformin on the Concentrations of Anti-Müllerian Hormone in Women with Polycystic Ovary Syndrome
Authors: Wang Liping , Hua Ke , Cai Wenwei , Fu Weiping , Zhang Hongxia , Fang Chunxia 
Published: October 2020

Abstract
Background: Whether treatment with oral contraceptives (OC) and metformin can reduce anti-Müllerian hormone (AMH) levels in PCOS remains controversial. This study aims to investigate the effects of OC and metformin on serum AMH concentrations in women with polycystic ovary syndrome (PCOS). Methods: This prospective study included 120 women with PCOS. Patients were treated with OC (35 μg of ethinyl estradiol plus 2 mg of cyproterone acetate), metformin, or OC plus metformin for 3 months according to their different endocrine/metabolic disturbances. Forty-eight patients with hyperandrogenism (HA) were treated with OC, 32 patients with insulin resistance (IR) were treated with metformin, and 40 patients with HA and IR were treated with a combination of OC and metformin. Serum AMH levels were compared before and after treatment within each group.Results: AMH levels decreased significantly in both OC groups (from 12.54 ± 5.59 ng/mL before treatment to 9.03 ± 4.49 ng/mL after treatment, P < 0.01) as well as in the OC + metformin group (from 10.62 ± 4.57 ng/mL to 7.74 ± 3.19 ng/mL, P < 0.01). However, AMH concentrations remained unchanged in the metformin group, although insulin sensitivity was improved. Conclusion: Treatment with OC alone or OC plus metformin led to a significant reduction of serum AMH in PCOS patients with HA, while metformin treatment alone did not affect AMH levels in patients with IR.Current Controlled Trials: ChiCTR2000038695The date of registration: 2020. 9. 28. Retrospectively registered.
A Systematic Review of the Effectiveness of Rehabilitation Therapy to Treat People with Chronic Knee Pain Following A Total Knee Replacement.
Authors: Johns Nathan Adam , Naylor Justine , Thirugnanam Brinda , Mckenzie Dean , Brady Bernadette , Olver John 
Published: October 2020

Abstract
Background:Chronic knee pain after a total knee replacement has been estimated to affect 10 to 30% of patients and is related to dissatisfaction with surgery, reduced function and reduced quality of life. Rehabilitation is often prescribed in the subacute period post-operatively, but it may offer benefit to the increasing numbers of patients with chronic pain after their knee replacement. The aim of this systematic review is to evaluate the effectiveness of rehabilitation to improve pain, function and quality of life in people with chronic knee pain persisting for more than 3 months following a total knee replacement.Methods: The systematic review was conducted following PRISMA guidelines with a search of the online databases Ovid Medline, Embase via Ovid, CINAHL Plus, PsycINFO, Ovid Emcare and Proquest from their earliest date to July 12, 2020. The search criteria included English language randomised controlled trials of rehabilitation strategies in any setting to treat people with chronic knee pain, defined as knee pain persisting for more than 3 months, following a total knee replacement. Rehabilitation programs included exercise therapy, patient education, cognitive and mind-body strategies and self-management and excluded medication trials, procedural techniques and complementary therapies. Results:There were 254 abstracts screened for eligibility with 13 remaining for full-text screening. Following full-text screening, there were no studies that met the eligibility criteria for evaluating rehabilitation therapy to treat chronic knee pain persisting for more than 3 months following a total knee replacement.Conclusion:Despite the high volume of knee replacement surgery and the high incidence of moderate to severe chronic pain ensuing, there is currently no evidence available that rehabilitation commencing three months after surgery can effectively treat chronic pain and disability following a total knee replacement.
Biomechanical Evaluation of Anterolateral Screw Fixation for Oblique Lumbar Interbody Fusion Surgery – A Finite Element Analysis
Authors: Fang gufang , Chen SG , zhuang wda , Huang WH , Sang Hongxun 
Published: October 2020

Abstract
Background: The most common complication of oblique lumbar interbody fusion (OLIF) is cage subsidence. OLIF combined with internal fixation could help decrease the cage subsidence and increase the fusion rate. The aim of this study was to evaluate the biomechanical feasibility and safety in the patients undergoing OLIF surgery with anterolateral screw fixation (ASF). Methods: Based on our previous validated model , L4-L5 functional surgical models corresponding to the ASF and Bilateral pedicle screw fixation(BPSF) methods were created. A 500 N compression force was applied to the superior surface of the model to represent the upper body weight, and a 7.5 Nm moment was applied to simulate the six movement directions of the lumbar spinal model: flexion/extension, right/left lateral bending and right/left axial rotation. Finite element (FE) models were developed to compare the biomechanics of the ASF and BPSF groups. Results: Compared to the range of motion (ROM) of the intact lumbar model, that of the ASF model was decreased by 82.0% in flexion, 60.0% in extension, and the BPSF model was decreased by 86.7% in flexion, 77.3% in extension. Compared to the BPSF model, the maximum stresses of the L4 inferior endplate (IEP) and L5 superior endplate (SEP) were greatly increased in the ASF model; The contact surface between vertebrae and screw (CSVS) in the ASF model produced nearly100% more stresses than the BPSF model in all moment .Conclusions: OLIF surgery with ASF could not reduce the maximum stresses on the endplate and CSVS, which may be a potential risk factor for cage subsidence and screw loosening.
Medium-Term Outcomes of Laparoscopic Pubocervical Fascia Reconstruction and Sacrospinous Ligament Fixation for Pelvic Organ Prolapse
Authors: Zhu Yunshan , Zhang Xiao , Chen Danxia , Li Guangxiao , Shang Shanliang , Li Jianqiong , Yang Jianhua 
Published: October 2020

Abstract
Background: The aim of this study was to investigate the medium-term outcomes of laparoscopic pubocervical fascia reconstruction and sacrospinous ligament fixation with a posterior approach for severe pelvic organ prolapse (POP). Methods: A total of 129 patients with severe POP quantitation stage III-IV who underwent laparoscopic pubocervical fascia reconstruction and sacrospinous ligament fixation with a posterior approach at Sir Run Run Shaw Hospital College of Medicine, Zhejiang University, Hangzhou, China between September 2014 and December 2018 were enrolled in this study. One hundred twenty-four patients were followed up for at least 1 year. The results and complications were recorded. Data were retrospectively reviewed. Results: All the patients underwent laparoscopic pubocervical fascia reconstruction and sacrospinous ligament fixation with a posterior approach. The average follow-up time was 32.27±12.90 months. The objective cure rate for those who underwent hysterectomy was 91.7 % (100/109), and no patient experienced apical vaginal prolapse. The objective cure rate for those who retained uterus was 66.67% (10/15). Suture-related complications were recorded.Conclusions: Laparoscopic pubocervical fascia reconstruction and sacrospinous ligament fixation with the posterior approach is a safe, minimally invasive, and effective method for patients with severe POP. Long-term follow-up is required to confirm the clinical effects.
Transcriptome Analysis of Thymus Tissues from Chinese Partridge Shank Chickens Before/After NDV LaSota Vaccine Injection Via High-Throughput RNA Sequencing
Authors: Nie Furong , Zhang Jingfeng , Li Mengyun , Chang Xuanniu , Duan Haitao , Li Haoyan , Zhou Jia , Ji Yudan , Guo Liangxing 
Published: October 2020

Abstract
Background: Newcastle disease (ND), caused by virulent Newcastle disease virus (NDV), is a huge threat for poultry and birds. LaSota strain of NDV is a common live attenuated vaccine to control ND. In this study, high-throughput RNA sequencing was performed to explore thymus tissue transcriptome change and better manage “vaccine failure” in Chinese Partridge Shank Chickens at 0 h and 48 h post LaSota vaccine injection. Results: 140 long non-coding RNAs (lncRNAs), 8 microRNAs (miRNAs) and 1514 mRNAs were differentially expressed post LaSota NDV vaccine inoculation. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis for vaccine-affected mRNAs and miRNA target genes was conducted. Moreover, 70 innate immune genes (31 up-regulated, 39 down-regulated) inGO, InnateDB and Reactome Pathway databases were found to be differentially expressed. Furthermore, the interaction network of proteins encoded by these dysregulated innate immune genes with known names was established by STRING website. Additionally, expression patterns of some dysregulated lncRNAs, miRNAs and mRNAs were further tested through RT-qPCR assay. Pearson correlation analysis for dysregulated transcripts validated by RT-qPCR assay was also performed. Based on the correlation data, 2 potential competing endogenous RNA (ceRNA) networks (ENSGALT00000060887/gga-miR-6575-5p/paraoxonase 1 (PON1) or MSTRG.188121.10/gga-miR-6631-5p/MMP9) were established. Conclusions: Our data presented many differentially expressed lncRNAs, miRNAs, mRNAs and innate immune genes, and established 2 potential ceRNA networks (lncRNAs/miRNAs/mRNAs) in Chinese Partridge Shank Chickens in response to LaSota vaccine inoculation, which could deepen our understanding on host responses to NDV LaSota vaccine in Chinese Partridge Shank Chickens.
Quick and Abnormal Ossification Results in No Growth Plate Formation and Skeletal Dysplasia in Postnatal Mice Induced by Conditional Knockout of Indian Hedgehog
Authors: Zhang Min , Sun Jian , Wei Xiaochun , Yuan Tao , Wang Chunfang , Dong Zhengquan , Wang Yunfei , Xiang Chuan , Li Pengcui 
Published: October 2020

Abstract
Objective: To observe the dynamic effect of conditional knockout of Indian hedgehog (Ihh) on ossification of postnatal growth plate, skeletal abnormalities and the related signaling factors in mice.Methods: P0 Col2a1-Cre ERT2; Ihhfl/fl mice were randomly divided into tamoxifen (TM) injection to delete Ihh and oil injection as control. Real-time PCR (RT-PCR) was used to detect Ihh knockout rate. X-ray and micro-CT were used to evaluate gross and knee joint morphology of 8-week-old mice. The hind knee joints were harvested at P2-P14 after the animals were euthanized to observe the dynamic evolution of the growth plate. Safranin-O and Von Kossa staining were performed to assess growth plate development and the mineralization of bone respectively. The costal cartilage cells of mice were cultured in vitro on postnatal day 6. Changes in the expression of parathyroid hormone-related protein (PTHrP) and bone morphogenetic protein 6 (BMP-6) were detected by immunohistochemistry and RT-PCR.Results: RT-PCR results confirmed that the knockout rate of Ihh was 76.83%. X-ray and micro-CT scans showed that the short-limb deformity of the experimental group was associated with abnormal development of the epiphysis. Saffron O staining showed a disorder of cell arrange in the growth-plate area of Ihhd/d mice; Von Kossa staining showed an early and premature growth-plate ossification that prevents the growth plate formation in Ihhd/d mice starting at P8. Immunohistochemistry and RT-PCR showed significantly decreased PTHrP expression (P<0.05) and significantly increased BMP-6 expression (P<0.05). Conclusion: Postnatal conditional knockout of Ihh inhibited the expression of PTHrP and promotes the expression of BMP-6, which leads to early and abnormal ossification of the growth plate, no growth plate formation, and skeletal dysplasia in mice.
An ionic control of metabolic-epigenetic reprogramming links inflammation to membrane potential-mediated nutrient acquisition
Authors: Wang Di , Yu Weiwei , Wang Zhen , Yu Xiafei , Chi Zhexu , Zhang Kailian , Chen Sheng , Xu Ting , Jiang Danlu , Guo Xingchen , Li Mobai , Zhang Jian , Fang Hui , Ye Qizhen , Yang Xuyan , Zhang Xue , Wu Yingliang , Yang Wei 
Published: October 2020

Abstract
Metabolic and epigenetic reprogramming play pivotal roles in driving inflammation, but the precise regulatory mechanisms remain minimally understood. Here we show an ionic control mediated by macrophage Kir2.1, an inwardly-rectifying K+ channel, promting lipopolysaccharide (LPS)-induced inflammation. Kir2.1 blockade by the selective inhibitor ML133 or its specific deletion in macrophages suppressed the production of LPS-induced inflammatory factors, such as interleukin-1β, and protected mice from LPS-induced sepsis in vivo. Kir2.1 loss-of-function led to a nutrient starvation phenotype, with impaired glucose and serine-glycine-one-carbon metabolism, whose synergy promotes the generation of S-adenosylmethionine (SAM). Accordingly, reduced SAM availability by Kir2.1 blockade decreased histone methylation at key inflammatory effector loci, such as Il1b. Although the immunomodulatory effect of Kir2.1 was independent of modulation by Ca2+ flux and general signaling pathways, its loss-of-function led to a depolarized membrane potential (Vm) which decreased the surface expression of nutrient transporters, including GLUT1 and CD98. We thus identify an ionic control of metabolic- epigenetic reprogramming that links inflammation to Vm-mediated nutrient acquisition and identifies potential new strategies for anti-inflammatory therapy.
24-h Urinary Sodium and Potassium and Their Association with Clinical Features of Migraine Headache
Authors: Arab Arman , Khorvash Fariborz , Heidari Zahra , Askari Gholamreza 
Published: October 2020

Abstract
Aim: There is a paucity of evidence regarding the association between sodium and potassium with migraine. To explore the possible relationship between 24-h urinary sodium and potassium with clinical features of migraine patients, we conducted a cross-sectional study using a sample of the Iranian population. Methods: In this cross-sectional study, 262 participants aged 20-50 years, with a diagnosis of migraine were included. One 24-h urine sample was collected by each subject to estimate sodium and potassium intake. Clinical features of migraine including frequency, duration, severity, migraine headache index score (MHIS), and headache impact test (HIT) were assessed. The serum nitric oxide values were assessed using the Griess method. Multiple linear regression analysis was used and beta (β) and 95% corresponding confidence interval (CI) were reported. Results: The 24-h urinary sodium was significantly associated with frequency (Model 3: β=1.86, 95%CI (0.10, 3.62); P=0.038), duration (Model 2: β=0.38, 95%CI (0.11, 0.66); P=0.006) and MHIS (Model 3: β=0.19, 95%CI (0.01, 0.37); P=0.034). Also, there was a significant association between Na/K ratio and MHIS (Model 2: β=0.16, 95%CI (0.003, 0.33); P=0.045). There was no significant relationship between 24-h urinary potassium and any of the intended variables (all P values <0.05). Conclusion: Our findings suggest that reducing sodium intake and increasing potassium intake could be used as a useful and novel approach to improve clinical findings of migraine and associated burden of disease. Additional studies are needed to replicate these findings and to discover mechanisms that mediate the association between sodium and potassium intake with migraine headache.
The fourth thoracic vertebra as a radiographic landmark for the depth of right internal jugular vein catheterisation in infants: A retrospective study
Authors: Liu Guoliang , Zhang Jianmin , Wang Fang , Liu Heqi 
Published: October 2020

Abstract
Backgroundː The carina is considered a reliable marker for the depth of right internal jugular vein catheterisation in infants. However, the carina is not always visible on chest radiographs, whereas vertebral bodies usually are. We performed a retrospective analysis of routine post-procedural chest radiographs of infants who had undergone congenital heart surgery and required central venous catheterisation. We evaluated the possibility of using vertebral bodies as radiographic landmarks for the identification of the depth of right internal jugular vein catheterisation.Methodsː In total, 108 infants (aged 1–12 months) who underwent congenital heart surgery from the 1st of January, 2019, to the 30th of June, 2019, were included. We analysed the post-operative chest radiographs of those who underwent right internal jugular vein catheterisation and assessed the visibility of the carina and vertebral bodies, as well as their positional relationship.Resultsː In total, 95 children were enrolled; in 61 (64%) cases, the carina was visible on the chest radiograph, whereas in 34 (36%) cases, it was not visible. In all 95 (100%) cases, the vertebral bodies were clearly visible. There was a significant difference between the rate of visibility of the carina and vertebral bodies (P=0.01 P<0.05). The carina was located at the third thoracic vertebra in one case (2%) and at the fourth thoracic vertebra in 60 cases (98%). There was a significant difference in the position of the carina (P=0.01, P<0.05).Conclusionsː Most fourth thoracic vertebrae were at the same level as the carina on chest radiographs. The fourth thoracic vertebra, therefore, has potential as a radiographic landmark for the depth of right internal jugular vein catheterisation in infants when the position of the carina is not visible on the chest radiograph.