bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication of biosynthesized nickel ferrites nanoparticles and evaluation of their insecticidal efficacy on beetles (Blaps polychresta) testicular integrity
Authors: Arafat Esraa A. , Eltaweil Abdelazeem S. , Abd El-Monaem Eman M. , Elhenawy Hanan I. , Hussein Hussein K. , El-Samad Lamia M. , Hassan Mohamed A. 
Published: February 2025

Abstract
Green synthesis of nanoparticles has emerged as a significant strategy to develop effective and eco-friendly insecticide agents to combat insecticide resistance and preserve environmental integrity and biodiversity. This study was thus designed to fabricate novel green synthesized NiFe2O4 nanoparticles (NiFe NPs) and investigate their potential insecticidal effects for the first time using Blaps polychresta beetle as an agricultural coleopteran pest model. Therefore, we prepared NiFe NPs following the hydrothermal synthesis procedure in the presence of lemon juice. The physiochemical characteristics of NiFe NPs were investigated employing SEM, TEM, FT-IR, XRD, TGA, VSM, and UV-Vis analysis. The lowest and most effective dose of NiFe NPs against male beetles was ascertained at a concentration of 0.03 mg/g body weight, reporting 67% mortality after 48 h. To study the insecticidal impact of NiFe NPs, EDX analysis demonstrated the bioaccumulation of NiFe NPs in testicular tissues of beetles, leading to pathophysiological consequences. Precisely, the oxidative stress incited by NiFe NPs led to disturbance of the antioxidant defense system, which was defined by augmentation of lipid peroxidation and suppression of antioxidant enzymes. Furthermore, the comet assay exhibited remarkable DNA impairment, while flow cytometry analysis showed substantial cellular necrosis and apoptosis in NiFe NPs-treated beetles compared to control insects. In correlation with these findings, several aberrations in the histological and ultrastructure attributes of testicular tissues were perceived, including impaired follicular and cyst walls, deteriorated parietal cells, necrosis, and vacuolations. These results implied that NiFe NPs triggered oxidative injury in the testes, resulting in male reproductive system dysfunction. Altogether, our findings accentuate the potential application of NiFe NPs as nanopesticides, paving the way for the sustainable and cost-effective management of insect pests in agriculture.
Carbon dioxide removal from triethanolamine solution using living microalgae-loofah biocomposites
Authors: Komkhum Tanakit , Sema Teerawat , Rehman Zia Ur , In-na Pichaya 
Published: February 2025

Abstract
Nowadays, the climate change crisis is an urgent matter in which carbon dioxide (CO2) is a major greenhouse gas contributing to global warming. Amine solvents are commonly used for CO2 capture with high efficiency and absorption rates. However, solvent regeneration consumes an extensive amount of energy. One of alternative approaches is amine regeneration through microalgae. Recently, living biocomposites, intensifying traditional suspended cultivation, have been developed. With this technology, immobilizing microalgae on biocompatible materials with binder outperformed the suspended system in terms of CO2 capture rates. In this study, living microalgae-loofah biocomposites with immobilized Scenedesmus acuminatus TISTR 8457 using 5%v/v acrylic medium were tested to remove CO2 from CO2-rich triethanolamine (TEA) solutions. The test using 1 M TEA at various CO2 loading ratios (0.2, 0.4, 0.6, and 0.8 mol CO2/mol TEA) demonstrated that the biocomposites achieved CO2 removal rates 3 to 5 times higher than the suspended cell system over 28 days, with the highest removal observed at the 1 M with 0.4 mol CO2/mol TEA (4.34 ± 0.20 gCO2/gbiomass). This study triggers a new exploration of integration between biological and chemical processes that could elevate the traditional amine-based CO2 capture capabilities. Nevertheless, pilot-scale investigations are necessary to confirm the biocomposites’s efficiency.
Fighting Zoom fatigue: Evidence-based approaches in university online education
Authors: Basch Johannes M. , Albus Patrick , Seufert Tina 
Published: February 2025

Abstract
As the world has rapidly transitioned to virtual platforms for education due to the Covid-19 pandemic, a new phenomenon known as “Zoom fatigue” has emerged. This term refers to the exhaustion associated with the extensive use of virtual communication platforms. In this context, the present study investigates the effects of different virtual class settings on cognitive load and fatigue. Four interventions were examined: self-view on vs. self-view off, focus-view function vs. grid-view function, activating a virtual background vs. natural background, and active participation vs. no active participation. The results suggest that turning off self-view can significantly reduce both cognitive load and fatigue. However, the effects of focus-view function vs. grid-view function did not reach significance for either cognitive load or fatigue. Similarly, using a virtual background vs. a natural background did not significantly affect fatigue, but it did influence cognitive load, with higher values for virtual backgrounds. Lastly, active participation in classes was associated with decreased fatigue and lower extraneous cognitive load compared to no active participation. The findings highlight the importance of considering both technological aspects (e.g., self-view, background settings) and pedagogical approaches (e.g., active participation) in online teaching to promote a more engaging and less fatiguing learning environment.
Involvement of propionate, citrulline, homoserine, and succinate in oral microbiome metabolite-driven periodontal disease progression
Authors: Ishihara Chikako , Sako Misato , Tsutsumi Kota , Fujii Narumi , Hashimoto Daiki , Sato Atsushi , Ichiba Yuko , Chikazawa Takashi , Kakizawa Yasushi , Nishinaga Eiji , Uchiyama Akira 
Published: February 2025

Abstract
Dysbiosis of the oral microbiome has been implicated in the onset and progression of periodontal diseases. An altered oral microbiome can significantly affect the concentration and composition ratio of bacterial-derived metabolites, thereby contributing to disease development. However, there is limited research on the role of metabolites derived from the oral microbiota. This study aimed to identify specific bacteria-derived metabolites and their contributions to pathogenicity. Mouth-rinsed water was collected from 24 patients with periodontal disease and 22 healthy individuals. We conducted a correlation analysis between periodontal disease-associated bacteria and metabolites present in mouth-rinsed water. We evaluated the effects of these metabolites on human gingival epithelial cells analysis of oral bacteria culture supernatants confirmed the origin of these metabolites. We identified 20 metabolites associated with bacteria that are significantly more prevalent in periodontal disease. Notably, propionate, succinate, citrulline, and homoserine—metabolites derived from the oral microbiome—were identified as being associated with periodontal disease. These results suggested that metabolites derived from the oral microbiota are involved in periodontal disease.
PTPRO represses breast cancer lung metastasis by inhibiting the JAK2-YAP axis
Authors: Xiong Xiao , Liu Jingfang , Wu Xiaotong , Yao Zhimeng , Meng Yuhua , Liu Shuang , Chen Yexi , Ren Hongzheng , Gao Shegan , Qiu Xiaofu , Zhang Hao 
Published: February 2025

Abstract
Lung metastasis is the primary cause of breast cancer-related mortality. Protein tyrosine phosphatases such as PTPRO are important in cancer progression. However, the role and underlying mechanisms of PTPRO in breast cancer lung metastasis are largely unknown. The function of PTPRO in breast cancer metastasis was examined in mice with ptpro deficiency driven by the PyMT promoter. The regulatory role of PTPRO in JAK2–YAP activation was tested in cell-based knockdown, overexpression and catalytic-dead mutation assays. Bioinformatics analyses and assays of human cancer specimens and mouse tumour samples were performed to investigate PTPRO-regulated pathways and functions. Ptpro deletion in MMTV-PyMT transgenic mice led to increased lung metastasis. Bioinformatics analyses and subsequent assays of human breast cancer specimens revealed a reverse correlation between PTPRO expression and JAK2–YAP pathway activity. Both in vitro and in vivo data demonstrated that PTPRO inactivates the JAK2–YAP pathway and diminishes the metastatic ability of breast cancer. Analysis of catalytic-dead PTPRO mutant breast cancer cells confirmed that functional PTPRO is a determinant of the activation of the JAK2–YAP pathway and the suppression of breast cancer metastasis. Data from patient, animal and cell-based models collectively demonstrated that PTPRO suppresses breast cancer lung metastasis by inhibiting JAK2–YAP dephosphorylation. Therefore, strengthening PTPRO or targeting PTPRO-mediated pathways could be potential strategies for inhibiting breast cancer lung metastasis.
A Stable and Dependable Visual Technique for On-Site Nipah Virus Nucleic Acids Detection
Authors: Chen Wencong , Cai Li , Ye Danni , Chen Jiahao , Ai Xueyan , Tang Xuehua , Deng Anqi , Gao Zihan , Xiang Meihua , Yu Mingen , Zhu Kun , Wang Maopeng 
Published: February 2025

Abstract
The Nipah Virus (NiV) is a zoonotic pathogen with the mortality rate of up to 75%, recurring in Asia over the past two decades. Due to increasing the risk of human transmission mediated by various intermediate hosts such as pigs and bats, it is necessary to produce an accurate and reliable point-of-care molecular detection method for NiV field diagnosis. In this study, we designed two pairs of primers targeting the conserved G and P genes and developed a point-of-care nucleic acid detection (POC-NAD) system by integrating one-step RT-PCR, lateral flow immunoassay, and microfluidic technologies. The POC-NAD system shows high specificity and sensitivity, with a Limit of Detection (LoD) of 199.1 copies/rxn. The primers aiming to the conserved sequences enables simultaneous detection of both NiV-M and NiV-B strains. Continuous evaluation of 21 simulated clinical samples demonstrated 100% concordance with RT-PCR results. Lateral flow-based visualization improves the display time and legibility of RT-PCR results. Additionally, microfluidic chips or chambers offer disposable reagent containers and consistent PCR amplification results across various field conditions. Therefore, the diagnostic tool is suitable for real-time nucleic acid testing and NiV surveillance in resource-limited field environments.
MiR-365-3p inhibits lung cancer proliferation and migration via CPT1A-mediated fatty acid oxidation
Authors: Xu Dan , Liu Bohong , Wang Lingling 
Published: February 2025

Abstract
The relationship between abnormal lipid acid metabolism and the progression of lung cancer is increasingly evident. Carnitine palmitoyltransferase 1A (CPT1A), a rate-limiting enzyme in fatty acid oxidation, has been implicated in the advancement of various cancers. However, the role of CPT1A in lung cancer and the regulatory mechanisms of microRNAs on CPT1A-mediated fatty acid oxidation remain largely unknown. In our study, we demonstrate that miR-365-3p inhibits CPT1A expression by targeting its 3’-untranslated region in lung cancer cells. The inhibition of CPT1A by miR-365-3p leads to increased lipid droplet accumulation, diminished ATP production, and a decrease in fatty acid oxidation levels. Furthermore, the disruption of fatty acid oxidation attenuates the ability of the miR-365-3p/CPT1A axis to modulate lung cancer cell proliferation and migration both in vitro and in vivo. Clinical data reveal that CPT1A expression is significantly upregulated while miR-365-3p is markedly downregulated. Additionally, there exists a negative correlation between miR-365-3p and CPT1A expression, and both are predictive of clinical outcome in lung cancer patients. Collectively, our findings shed light on the function and mechanistic pathway of the miR-365-3p/CPT1A axis in lung cancer, which might provide a potential therapeutic target for lung cancer.
A novel voice in head actor critic reinforcement learning with human feedback framework for enhanced robot navigation
Authors: Sharma Alabhya , Balasundaram Ananthakrishnan , Shaik Ayesha , Vaithilingam Chockalingam Aravind 
Published: February 2025

Abstract
This work presents a novel Voice in Head (ViH) framework, that integrates Large Language Models (LLMs) and the power of semantic understanding to enhance robotic navigation and interaction within complex environments. Our system strategically combines GPT and Gemini powered LLMs as Actor and Critic components within a reinforcement learning (RL) loop for continuous learning and adaptation. ViH employs a sophisticated semantic search mechanism powered by Azure AI Search, allowing users to interact with the system through natural language queries. To ensure safety and address potential LLM limitations, the system incorporates a Reinforcement Learning with Human Feedback (RLHF) component, triggered only when necessary. This hybrid approach delivers impressive results, achieving success rates of up to 94.54%, surpassing established benchmarks. Most importantly, the ViH framework offers a modular and scalable architecture. By simply modifying the environment, the system demonstrates the potential to adapt to diverse application domains. This research provides a significant advancement in the field of cognitive robotics, paving the way for intelligent autonomous systems capable of sophisticated reasoning and decision-making in real-world scenarios bringing us one step closer to achieving Artificial General Intelligence.
Global atmospheric distribution of microplastics with evidence of low oceanic emissions
Authors: Yang Shanye , Brasseur Guy , Walters Stacy , Lichtig Pablo , Li Cathy W. Y. 
Published: February 2025

Abstract
Recent investigations based on sea–air transfer physical mechanistic studies suggest that the global ocean’s contribution to atmospheric microplastic emissions is significantly lower (four orders of magnitude) than previously estimated. However, no atmospheric models or observations have yet validated this lower emission flux, leaving the analysis without adequate validation and practical significance. Here, we provide quantitative estimates of the global atmospheric microplastic budget based on this reduced oceanic flux. Our model aligns well with observed atmospheric microplastic concentrations and suggests that the ocean functions more as a sink than a source, contributing only ~0.008% of global emissions but accounting for ~15% of total deposition. This challenges the previous view of the ocean as the primary atmospheric microplastic source, urging a reassessment of pollution mitigation strategies.
A scoping review of ethical aspects of public-private partnerships in digital health
Authors: Bak Marieke A. R. , Horbach Daan , Buyx Alena , McLennan Stuart 
Published: February 2025

Abstract
Partnerships between public and private organizations in digital health can promote more accessible, affordable, and high-quality care, but they also raise ethical and governance challenges. We searched PubMed, EMBASE, and Web of Science, identifying 46 studies examining ethical aspects of digital health public-private partnerships (PPPs). Three key themes emerged: data privacy and consent, ensuring public benefit and access, and good governance and demonstrating trustworthiness. We provide recommendations for each theme. To foster responsible innovation, we conclude that early and contextual operationalisation of ethics guidelines in PPPs is necessary to balance respect for fundamental values with the pursuit of impactful innovation. If PPPs become more successful as a result, this contributes to reducing the research waste of failed collaborations. Further research should clarify the scope of PPPs and definition of ‘public benefit’, and we call for critical study on the ‘economization’ of digital health promoted by public and private sector organizations.
High-performance magnetostatic wave resonators based on deep anisotropic etching of gadolinium gallium garnet substrates
Authors: Tiwari Sudhanshu , Ashok Anuj , Devitt Connor , Bhave Sunil A. , Wang Renyuan 
Published: February 2025

Abstract
Magnetostatic wave resonators based on yttrium iron garnet (YIG) are a promising technology platform for future communication filters. Such devices have demonstrated better quality factors than acoustic resonators in the 7 GHz range and above. However, the coupling coefficients of these resonators have been limited to less than 3%, primarily due to the restricted design space that is a result of microfabrication challenges related to the patterning of gadolinium gallium garnet (GGG), the substrate material used for growing single-crystal YIG. Here we report magnetostatic wave resonators created through the anisotropic etching of GGG substrates. Our approach, which is based on the YIG-on-GGG platform, uses a transducer with a hairclip-like structure. It is created by developing a microfabrication methodology that involves thinning and deep etching (up to 100 μm) of the GGG substrate. The resulting magnetostatic wave resonators exhibit a coupling of more than 8% in the 6–20 GHz frequency range.
Hydrodynamic cavitation mediated Spirulina valorisation with insights into phycocyanin extraction and biogas production
Authors: Chegukrishnamurthi Madhubalaji , Nagarajan Sanjay , Ravi Sarada , Mudliar Sandeep Narayana , Ranade Vivek V. 
Published: February 2025

Abstract
Commercial phycocyanin extraction is energy-intensive and lacks scalability. Alternatively, this study reports the systematic investigation of hydrodynamic cavitation for intensified phycocyanin extraction from Spirulina. Additionally, biomethane potential of the residual biomass, obtained after phycocyanin extraction was also investigated. The biomethane generation rate decreased with an increasing number of passes while the biomethane potential remained unaffected. To reliably compare phycocyanin extraction across systems, dimensionless normalised yields were defined. A normalised phycocyanin yield of 4.3 (52 mg phycocyanin g−1) at an inlet pressure of 150 kPa and 90 passes was identified (optimum cavitation). Lowest specific energy input (0.06kWh kg−1) was calculated for processing 100 g L−1
Spirulina, which is one to two orders of magnitude lower than current state-of-the-art. Furthermore, a net energy gain of 600-2497kWh kg−1 obtained from biomethane generation showcased a viable Spirulina biorefinery, intensified via hydrodynamic cavitation. This work provides a route for phycocyanin extraction with significantly reduced energy input and potential for wider bioproduct extraction and biorefining from a range of biomasses via hydrodynamic cavitation.
Repetitive concussions promote microglia-mediated engulfment of presynaptic excitatory input associated with cognitive dysfunction
Authors: Chahin Maryam , Mutschler Julius , Dzhuleva Stephanie P. , Dieterle Clara , Jimenez Leidy Reyes , Bhattarai Srijan Raj , Van Steenbergen Valerie , Bareyre Florence M. 
Published: February 2025

Abstract
Concussions are a current health concern and account for the vast majority of head trauma. While symptoms after a single impact are usually transient, repetitive concussions, as often occur in sports, are responsible for persistent acute and chronic deficits. Here, we used a model of bilateral midline-centered concussions in mice to show that repetitive concussions selectively induce impairments in learning ability compared to single-impact injuries. Since microglial cells and their activation are considered key factors in degenerative pathology after brain trauma, we examined their structure and function after single and repetitive concussions in the cortex underlying the concussions and in the hippocampus. We found that only repetitive concussions led to a significant long-lasting structural activation of microglia and an increase in microglia-mediated engulfment of presynaptic excitatory synapses, while the elimination of inhibitory synapses was not altered. Since the density of excitatory input did not change during the 6-week study period, we hypothesize that there is a turnover of excitatory synapses following repetitive concussion that can be compensated for, anatomically but not behaviorally.
Metabolic control analysis of biogeochemical systems
Authors: Louca Stilianos 
Published: February 2025

Abstract
Many reactive systems involve processes operating at different scales, such as hydrodynamic transport and diffusion, abiotic chemical reactions, microbial metabolism, and population dynamics. Determining the influence of these processes on system dynamics is critical for model design and for prioritizing parameter estimation efforts. Metabolic control analysis is a framework for quantifying the role of enzymes in cellular biochemical networks, but its applicability to biogeochemical and other reactive systems remains unexplored. Here I show how the core concepts of metabolic control analysis can be generalized to much more complex reactive systems, enabling insight into the roles of physical transport, population dynamics, and chemical kinetics at organismal to planetary scales. I demonstrate the power of this framework for two systems of importance to ocean biogeochemistry: A simplified (mostly didactic) model for the sulfate methane transition zone in Black Sea sediments, and a more comprehensive model for the oxygen minimum zone in Saanich Inlet near steady state. I find that physical transport is by far the greatest rate-limiting factor for sulfate-driven methane oxidation in the first system and for fixed nitrogen loss in the second system.
Medical ontology learning framework to investigate daytime impairment in insomnia disorder and treatment effects
Authors: Büsser Alexander J. , Durrer Renato , Freidank Moritz , Togninalli Matteo , Olivieri Antonio , Grandner Michael A. , McCall William V. 
Published: February 2025

Abstract

Background
Specificity challenges frequently arise in medical ontology used for the representation of real-world data, particularly in defining mental health disorders within widely used classification systems such as the International Classification of Diseases (ICD). This study aims to address these challenges by introducing the Disease-Specific Medical Ontology Learning (DiSMOL) framework, designed to generate precise disease representations from clinical physician notes, with a focus on daytime impairment in insomnia disorder.

Methods
The study applied the Disease-Specific Medical Ontology Learning framework to clinical notes to better represent daytime impairment. The framework’s performance was compared to insomnia expert-selected codes from ICD. Key statistical methods included sensitivity and F1-score comparisons, as well as analysis of symptom changes after the use of various medications, including benzodiazepines, non-benzodiazepine receptor agonists, and trazodone.

Results
The DiSMOL framework significantly enhances the identification of daytime impairment in people with insomnia. Sensitivity increases from 17% to 98%, and the F1-score improves from 28% to 86%, compared with expert-selected ICD codes. Additionally, the framework reveals significant increases in daytime impairment symptoms following benzodiazepine use (18.9%), while traditional ICD codes do not detect any significant change.

Conclusions
The study demonstrates that DiSMOL offers a more accurate method for identifying specific disease aspects, such as daytime impairment in insomnia, than traditional coding systems. These findings highlight the potential of specialized ontologies to enhance the representation and analysis of real-world clinical data, with important implications for healthcare policy and personalized medicine.