bluuebunny/crossref_metadata_2025_split huggingface dataset:
GoPerio - impact of a personalized video and an automated two-way text-messaging system in oral hygiene motivation: study protocol for a randomized controlled trial
Authors: Garyga Valentin , Pochelu Florian , Thivichon-Prince Béatrice , Aouini Walid , Santamaria Julie , Lambert France , Maucort-Boulch Delphine , Gueyffier Francois , Gritsch Kerstin , Grosgogeat Brigitte 
Published: December 2019

Abstract
Background
Oral hygiene is of paramount importance for the preservation of oral health, and for patients affected by periodontal disease establishing an effective oral hygiene routine is the first step of therapy. Several clinical frameworks have been developed to foster behavior change, such as motivational interviewing. However, two obstacles can be identified. First, patients tend to forget the advice they were given during the consultation. Second, it is hard to maintain motivation in the long term, thus leading to relapse. An innovative eHealth solution was designed with the aim to tackle both obstacles and supplement the current clinical standard of care. The primary objective is to compare the full mouth plaque scores of study groups (eHealth plus standard of care versus standard of care only) at 8 weeks of follow up. The main secondary objective is to compare the full mouth bleeding score at 8 weeks of follow up.

Methods/design
The “GoPerio” study is a multicenter, randomized, controlled trial assessing the impact of a novel eHealth concept for oral hygiene motivation (personalized video of oral hygiene routine available for the patient via a cloud server plus interactive text messages) in addition to the current standard of care (motivational interviewing plus tooth scaling and polishing). The minimum sample size required is 86 patients. Participants will be randomized (allocation ratio 1:1): test group (eHealth plus standard of care) versus control group (standard of care only). The primary outcome is oral hygiene as measured by the full mouth (six sites per tooth) plaque control record (PCR) index. The main secondary outcome is gingival inflammation as measured by the full mouth (six sites per tooth) bleeding on probing (BOP) index. Both the primary and the main secondary outcomes are evaluated by blinded and calibrated examiners at 8 weeks of follow up. The other secondary outcomes are patient satisfaction and patient behavior change and motivation.

Discussion
The study will investigate the value of an innovative eHealth approach to strengthen patient motivation for oral hygiene. If proven effective, such an approach would supplement the current clinical standard of care, resulting in improved clinical outcomes with negligible impact on productivity in a dental practice.

Trial registration
ClinicalTrials.gov, NCT03109808. Registered on 12 April 2017.
Sponsor: Hospices Civils de Lyon. BP 2251, 3 quai des Célestins, 69,229 Lyon cedex 02.
Protocol version: 1.0 as of 21 September 2016.
Effects of larval diets and temperature regimes on life history traits, energy reserves and temperature tolerance of male Aedes aegypti (Diptera: Culicidae): optimizing rearing techniques for the sterile insect programmes
Authors: Sasmita Hadian Iman , Tu Wu-Chun , Bong Lee-Jin , Neoh Kok-Boon 
Published: December 2019

Abstract
Background
Producing high quality sterile males is vital in Aedes aegypti rear-and-release birth control strategies. Larval diets, rearing temperatures, and their interactions determine the accumulation rates of essential nutrients in larvae, but these factors have been understudied in relation to mass-rearing techniques for producing eminent males.

Methods
We compared the effects of two larval diets, a cereal-legume-based diet (Khan’s diet) and a standard larval diet developed in the FAO/IAEA Insect Pest Control Laboratory (IAEA 2 diet). Diets were tested at selected temperatures for both larval and male adult life history traits, adult extreme temperature tolerance, and mating capacity relative to energy reserves of reared male adult Ae. aegypti.

Results
Khan’s diet resulted in shorter immature development time at each test temperature (except for 25 °C) than an IAEA 2 diet. Larvae reared at 28 °C and 32 °C with Khan’s diet demonstrated low pupation rates (c.80%). We accounted for these phenomena as secondary sex ratio manipulation, because a higher proportion of male adults emerged at 28 °C and 32 °C than that for the IAEA 2 diet. In general, the pupal development time shortened as temperature increased, resulting in higher teneral energy reserves in male mosquitoes. High energy reserves allowed male mosquitoes reared with Khan’s diet to have higher adult longevity (5–6 days longer when sugar-fed and 2–3 days longer when water-fed) and tolerance of heat stress than those fed on the IAEA 2 diet. The IAEA 2 diet produced larger male mosquitoes than Khan’s diet did: mosquitoes fed on Khan’s diet were 1.03–1.05 times smaller than those fed on the IAEA 2 diet at 28 °C and 32 °C. No evidence indicated reduced mating capacity for small mosquitoes fed on Khan’s diet.

Conclusions
Larvae reared at 28 °C and 32 °C with Khan’s diet were characterized by shorter immature development time compared with those fed on the IAEA 2 diet. Adult mosquitoes produced from that larval rearing condition exhibited a significant male bias, long lifespan, and better endurance against extreme temperatures relative to energy reserves. Thus, the larval diet at rearing temperature of 28 °C and 32 °C optimized rearing techniques for the sterile insect programmes. However, mating competitiveness and flight performance of adult males require further investigation.
Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling
Authors: Kong Yuanyuan , Hu Xiaoli , Zhong Yingqun , Xu Ke , Wu Buling , Zheng Jianmao 
Published: December 2019

Abstract
Background
Magnesium (Mg2+)-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells (DPSCs), but the regulatory mechanisms remain undefined. The aim of this work was to assess magnesium’s function in the above process and to explore the associated signaling pathway.

Methods
DPSCs underwent culture in odontogenic medium with the addition of 0, 1, 5, or 10 mM MgCl2. Intracellular Mg2+ levels in DPSCs were evaluated flow cytometrically using Mag-Fluo-4-AM. Mg2+-entry was inhibited by TRPM7 inhibitor 2-aminoethoxydiphenyl borate (2-APB). RNA-Sequencing was carried out for assessing transcriptome alterations in DPSCs during odontogenic differentiation associated with high extracellular Mg2+. KEGG pathway analysis was performed to determine pathways related to the retrieved differentially expressed genes (DEGs). Immunoblot was performed for assessing magnesium’s role and exploring ERK/BMP2/Smads signaling.

Results
Mg2+-enriched microenvironment promoted odontogenic differentiation in DPSCs via intracellular Mg2+ increase. Consistently, the positive effect of high extracellular Mg2+ on odontogenic differentiation in DPSCs was blocked by 2-APB, which reduced Mg2+ entry. RNA-sequencing identified 734 DEGs related to odontogenic differentiation in DPSCs in the presence of high extracellular Mg2+. These DEGs participated in many cascades such as MAPK and TGF-β pathways. Consistently, ERK and BMP2/Smads pathways were activated in DPSCs treated with high extracellular Mg2+. In agreement, ERK signaling inhibition by U0126 blunted the effect of high extracellular Mg2+ on mineralization and odontogenic differentiation in DPSCs. Interestingly, BMP2, BMPR1, and phosphorylated Smad1/5/9 were significantly decreased by U0126, indicating that BMP2/Smads acted as downstream of ERK.

Conclusions
Mg2+-enriched microenvironment promotes odontogenic differentiation in DPSCs by activating ERK/BMP2/Smads signaling via intracellular Mg2+ increase. This study revealed that Mg2+-enriched microenvironment could be used as a new strategy for dental pulp regeneration.
Visual contour tracking based on inner-contour model particle filter under complex background
Authors: Cao Songxiao , Wang Xuanyin 
Published: December 2019

AbstractIn this paper, a novel particle filter–based visual contour tracking method is proposed, which uses inner-contour model to track contour object under complex background. The purpose is to achieve effectiveness and robustness against complex background. To that end, the proposed method first utilized Sobel edge detector to detect the edge information along the normal line of the contour. Then, it sampled the inner part of the normal line to get the local color information, which was then combined with the edge information to construct new normal line likelihood. After that, all the inner color information was used to construct global color likelihood. Finally, the edge information, local color information, and global color information were fused into new observation likelihood. Experimental results showed that the proposed method was robust for contours tracking under complex background, and it was also computationally efficient and can run in real-time completely.
Operators constructed by means of basic sequences and nuclear matrices
Authors: Morsy Ahmed , Faried Nashat , Harisa Samy A. , Nisar Kottakkaran Sooppy 
Published: December 2019

AbstractIn this work, we establish an approach to constructing compact operators between arbitrary infinite-dimensional Banach spaces without a Schauder basis. For this purpose, we use a countable number of basic sequences for the sake of verifying the result of Morrell and Retherford. We also use a nuclear operator, represented as an infinite-dimensional matrix defined over the space $\ell _{1}$ℓ1 of all absolutely summable sequences. Examples of nuclear operators over the space $\ell _{1}$ℓ1 are given and used to construct operators over general Banach spaces with specific approximation numbers.
In vitro and in vivo anti-aging effects of compounds isolated from Artemisia iwayomogi
Authors: Kim Kyung Young , Lee Eun Ju , Whang Wan Kyunn , Park Cho Hi 
Published: December 2019

AbstractIn Korean folk medicine, Artemisia iwayomogi has largely been employed for the improvement of diabetic complications and hepatic function as well as in the treatment of female diseases and skin whitening. Accordingly, the present study sought to assess cosmeceutical activity of Artemisia iwayomogi. Through activity-guided fractionation, scopolin (AI-1), 2,4-dihydroxy-6-methoxy-acetophenone 4-O-β-D-glucoside (AI-2), scopoletin (AI-3), kaempferol-3-O-methyl ether (AI-4) and luteolin (AI-5) were isolated from ethyl acetate and water fractions of Artemisia iwayomogi. Of them, AI-4 and AI-5 exhibited strong antioxidative and whitening effects. In addition, AI-1, AI-3, AI-4, and AI-5 demonstrated considerable anti-wrinkle performances. According to results of quantitative analysis of compounds isolated from Artemisia iwayomogi, scopolin (AI-1) is a major component of Artemisia iwayomogi. Moreover, O/W type cream made by water fraction which included scopolin showed unwrinkling effect for 8 weeks when it was applied to face in an in vivo study. Specific contributions of Artemisia iwayomogi were as follows: AI-1, 1.21 mg/g; AI-2, 0.26 mg/g; AI-3, 0.38 mg/g; AI-4, 0.01 mg/g; and AI-5, 0.04 mg/g.
Diurnal variation of salivary oxidative stress marker 8-hydroxyguanine
Authors: Watanabe Sintaroo , Kawasaki Yuya , Kawai Kazuaki 
Published: December 2019

Abstract
Introduction
Oxidative stress is a risk factor for life-style related diseases, including cancer. We recently reported that the oxidative stress marker 8-hydroxyguanine (8-OHGua) can be measured in saliva non-invasively. Understanding the diurnal pattern of salivary 8-OHGua levels is crucial for evaluating the oxidative stress. In this study, we analyzed the diurnal variation of salivary 8-OHGua levels.

Findings
The salivary 8-OHGua levels were relatively stable in the daytime (10:00–22:00). The daytime 8-OHGua levels seemed to represent the individual oxidative stress status. The average amount and the variation of the salivary 8-OHGua levels immediately after awakening were higher than those of the daytime levels.

Conclusions
The 8-OHGua levels in saliva exhibited diurnal variation. The levels were higher at the time of awakening. At this point, the daytime levels of salivary 8-OHGua may be appropriate for evaluating the individual oxidative stress status. Further study is needed for understanding and utilizing the 8-OHGua levels at the time of awakening.
The added value of18F-FDG PET/CT in staging non-small cell lung cancer
Authors: Sheha Aliaa S. , Elia Remon Zaher , Ghoneim Nada Mohammed Farid Hassan 
Published: December 2019

Abstract
Background
Non-small cell lung cancer (NSCLC) accounts for about 80% of all lung cancers. The current criteria for its staging are based on the TNM system that determines treatment options and predicts survival rate in patients. The aim of the study was to evaluate the diagnostic accuracy of 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography PET/CT in staging of NSCLC patients.

Methods
A retrospective study was conducted. We reviewed the CT and PET/CT examinations done in our institution on pathologically proven patients of NSCLC, in the period between October 2018 and end of July 2019.

Results
A total of 40 patients were evaluated with the age ranging from 37 to 77 years old, and the mean was 55.63 years (SD ± 10.29). There were 31 male cases and 9 female cases. When we compared contrast enhanced CT (CECT) to PET-CT for staging, PET-CT helped upstage disease in 10 of 40 patients (25%) and downstage in 3 of 40 patients (7.5%).

Conclusion
PET/CT is a useful imaging tool in initial staging of the newly diagnosed patients with NSCLC. It is better thаn СT alone fоr detection of malignant lesions for accurate staging. It can change the strategy of treatment according to its findings.
Preoperative MRI of perianal fistula evaluation and its impact on surgical outcome
Authors: Algazzar Hesham Youssef , Eldib Diaa Bakry , Bahram Mahmoud A. , Zaher Nasser A. 
Published: December 2019

Abstract
Background
Perianal fistula (PAF) is an abnormal tract communicating an external cutaneous opening in the perianal region to the anal canal. PAF is one of the common anorectal disorders in surgical practice with high prevalence. The current study aimed to determine the ability of preoperative MRI for preoperative evaluation of perianal fistula.

Results
This is a retrospective evaluation of 65 patients with perianal fistula. MRI fistula imaging-related data were revised, and fistula severity was scored using criteria of both local extension of fistulas and active inflammation for a total maximum score of 22. Preoperative MRI could predict the severity of perianal disease with sensitivity, specificity, and accuracy rates of 75%, 92%, and 84.6%, respectively. Surgical findings concerning PAF severity correlated significantly with MRI findings. Diffusion-weighted magnetic resonance imaging (DW-MRI) provided high sensitivity and accuracy with 100% specificity for fistula visualization and highest sensitivity, specificity, and accuracy for detection of cavities > 3 mm in diameter. DW-MRI provided the highest specificity rate on ROC curve analysis among the three MRI pulse sequences (DW-MRI, short tau inversion recovery (STIR), and T1 post-contrast).

Conclusion
MRI is valuable and accurate for preoperative investigation for PAF evaluation and abscess localization. MRI allowed accurate detection of internal fistula opening and its relation to sphincters. DW-MRI is a valuable sequence with highest diagnostic yield, and its addition to STIR WI improves sensitivity and specificity for determination of fistula activity and extension.
The impact of bubble curtains on seismic air-gun signatures and its high-frequency emission
Authors: Wehner Daniel , Landrø Martin 
Published: December 2019

In marine seismic acquisition, air guns are the most common source and, in recent years, research on their impact on the marine environment has increased. The main focus is on the reduction of emitted high frequencies, approximately greater than 200 Hz, which are normally not useful for seismic imaging. Therefore, potential ways to reduce the high frequencies from air guns are investigated and the development of alternative source types has increased. We have investigated the impact of bubble curtains on the source signature from seismic air guns because bubble curtains are known to mitigate high frequencies in other applications, e.g., pile driving for offshore wind farms. We have conducted tank experiments with two different configurations of bubble curtains around a single air gun and compared the results to the conventional source signature without a bubble curtain. The two different bubble curtains vary in size and in the way they are attached to the air gun. The amount of injected air into the bubble curtains is varied for both configurations. We compare the measured results to simulated data using a common model for air-gun source signatures. The results indicate a reduced peak amplitude with increasing air injection through the bubble curtain. This corresponds to a gradually decreasing frequency content for frequencies greater than 50 Hz. The frequencies of the source signal of less than 50 Hz are practically unaffected by the bubble curtain. In addition, the bubble time period of the source signal is slightly increased with an increasing amount of air injection through the bubble curtain. The main cause for the reduced peak amplitude is likely to be a buffer effect of the bubble curtain on the released air. Hence, a bubble curtain concentrated around the air-gun ports could be an efficient and practical solution to reduce the high-frequency acoustic emission from air guns.
Mutation of SGK3, a Novel Regulator of Renal Phosphate Transport, Causes Autosomal Dominant Hypophosphatemic Rickets
Authors: Cebeci Ayşe Nurcan , Zou Minjing , BinEssa Huda A , Alzahrani Ali S , Al-Rijjal Roua A , Al-Enezi Anwar F , Al-Mohanna Futwan A , Cavalier Etienne , Meyer Brian F , Shi Yufei 
Published: December 2019

Abstract

Context
Hypophosphatemic rickets (HR) is a group of rare hereditary renal phosphate wasting disorders caused by mutations in PHEX, FGF23, DMP1, ENPP1, CLCN5, SLC9A3R1, SLC34A1, or SLC34A3.

Objective
A large kindred with 5 HR patients was recruited with dominant inheritance. The study was undertaken to investigate underlying genetic defects in HR patients.

Design
Patients and their family members were initially analyzed for PHEX and FGF23 mutations using polymerase chain reaction sequencing and copy number analysis. Exome sequencing was subsequently performed to identify novel candidate genes.

Results
PHEX and FGF23 mutations were not detected in the patients. No copy number variation was observed in the genome using CytoScan HD array analysis. Mutations in DMP1, ENPP1, CLCN5, SLC9A3R1, SLC34A1, or SLC34A3 were also not found by exome sequencing. A novel c.979–96 T&gt;A mutation in the SGK3 gene was found to be strictly segregated in a heterozygous pattern in patients and was not present in normal family members. The mutation is located 1 bp downstream of a highly conserved adenosine branch point, resulted in exon 13 skipping and in-frame deletion of 29 amino acids, which is part of the protein kinase domain and contains a Thr-320 phosphorylation site that is required for its activation. Protein tertiary structure modelling showed significant structural change in the protein kinase domain following the deletion.

Conclusions
The c.979–96 T&gt;A splice mutation in the SGK3 gene causes exon 13 skipping and deletion of 29 amino acids in the protein kinase domain. The SGK3 mutation may cause autosomal dominant HR.
Tissue transglutaminase 2 regulates tumor cell tensional homeostasis by increasing contractility
Authors: Bordeleau Francois , Wang Wenjun , Simmons Alysha , Antonyak Marc A. , Cerione Richard A. , Reinhart-King Cynthia A. 
Published: December 2019

ABSTRACT
Abnormal tensional cellular homeostasis is now considered a hallmark of cancer. Despite this, the origin of this abnormality remains unclear. In this work, we investigated the role of tissue transglutaminase 2 (TG2, also known as TGM2), a protein associated with poor prognosis and increased metastatic potential, and its relationship to the EGF receptor in the regulation of the mechanical state of tumor cells. Remarkably, we observed a TG2-mediated modulation of focal adhesion composition as well as stiffness-induced FAK activation, which was linked with a distinctive increase in cell contractility, in experiments using both pharmacological and shRNA-based approaches. Additionally, the increased contractility could be reproduced in non-malignant cells upon TG2 expression. Moreover, the increased cell contractility mediated by TG2 was largely due to the loss of EGFR-mediated inhibition of cell contractility. These findings establish intracellular TG2 as a regulator of cellular tensional homeostasis and suggest the existence of signaling switches that control the contribution of growth factor receptors in determining the mechanical state of a cell.
Kinetic volume analysis on dynamic contrast-enhanced MRI of triple-negative breast cancer: associations with survival outcomes
Authors: Hayashi Yoko , Satake Hiroko , Ishigaki Satoko , Ito Rintaro , Kawamura Mariko , Kawai Hisashi , Iwano Shingo , Naganawa Shinji 
Published: December 2019

Objective:
To evaluate the associations between computer-aided diagnosis (CAD)-generated kinetic volume parameters and survival in triple-negative breast cancer (TNBC) patients.

Methods:
40 patients with TNBC who underwent pre-operative MRI between March 2008 and March 2014 were included. We analyzed CAD-generated parameters on dynamic contrast-enhanced MRI, visual MRI assessment, and histopathological data. Cox proportional hazards models were used to determine associations with survival outcomes.

Results:
12 of the 40 (30.0%) patients experienced recurrence and 7 died of breast cancer after a median follow-up of 73.6 months. In multivariate analysis, higher percentage volume (%V) with more than 200% initial enhancement rate correlated with worse disease-specific survival (hazard ratio, 1.12; 95% confidence interval, 1.02–1.22; p-value, 0.014) and higher %V with more than 100% initial enhancement rate followed by persistent curve type at 30% threshold correlated with worse disease-specific survival (hazard ratio, 1.33; 95% confidence interval, 1.10–1.61; p-value, 0.004) and disease-free survival (hazard ratio, 1.27; 95% confidence interval, 1.12–1.43; p-value, 0.000).

Conclusion:
CAD-generated kinetic volume parameters may correlate with survival in TNBC patients. Further study would be necessary to validate our results on larger cohorts.

Advances in knowledge:
CAD generated kinetic volume parameters on breast MRI can predict recurrence and survival outcome of patients in TNBC. Varying the enhancement threshold improved the predictive performance of CAD generated kinetic volume parameter.
'Untruss a Point' - Interiority, Sword Combat, and Gender in <i>The Roaring Girl</i>
Authors: Carter Matthew Charles 
Published: December 2019

This article discusses the critical apparatus surrounding Dekker and Middleton’s well-known play The Roaring Girl. While previous discussions of the text have focused mostly on Moll’s cross-dressing, I instead look at Moll’s sword skills to show how the lascivious behaviour of London’s men produces her gender performance, which seems unruly by early modern standards. I also examine other rituals of gender construction that texture previous analyses of Moll.