bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow Cytometric Assessment of AKT Signaling in Platelet Activation: An Alternative Diagnostic Tool for Small Volumes of Blood
Authors: Althaus K. , Wagner M. , Marini I. , Bakchoul T. , Pelzl L. 
Published: November 2020

Abstract
Introduction The diagnosis of platelet function disorder in children is challenging. Light transmission aggregometry is the gold standard for platelet function disorders. However, large blood volumes are required. Currently, there are no existing tools for the diagnosis of platelet function disorders that use small blood volumes. AKT signaling plays a central role in platelet activation during hemostasis and might be visualized by flow cytometry.
Methods Platelet-rich plasma obtained by centrifugation of citrated blood from healthy volunteers was activated with arachidonic acid, thrombin receptor activating peptide-6 (TRAP-6), collagen, adenosine diphosphate ADP, collagen-related peptide (CRP), and epinephrine. After platelet activation, the phosphorylation of AKT was assessed by flow cytometer using a Navios cytometer.
Results Healthy volunteers showed a reproducible phosphorylation of AKT upon activation. In comparison to nonactivated platelets, we documented an increase in pAKT expression with all agonists. Especially TRAP-6 and CRP caused considerable increase in percentage of pAKT expression throughout all the tested healthy volunteers.
Conclusion An activation of the AKT-signal pathway by different agonists can clearly be detected on the flow cytometer, indicating that the visualization of signaling in platelets by flow cytometry might be an efficient alternative for light transmission aggregometry to test platelet function in children.
Insights from the Hereditary Thrombotic Thrombocytopenic Purpura Registry: Discussion of Key Findings Based on Individual Cases from Switzerland
Authors: Kremer Hovinga Johanna A. , Braschler Thomas R. , Buchkremer Florian , Farese Stefan , Hengartner Heinz , Lovey Pierre-Yves , Largiadèr Carlo R. , Mansouri Taleghani Behrouz , Tarasco Erika 
Published: November 2020

AbstractThe Hereditary TTP Registry is an international cohort study for patients with a confirmed or suspected diagnosis of hereditary thrombotic thrombocytopenic purpura (hTTP) and their family members. Hereditary TTP is an ultra-rare blood disorder (prevalence of ∼1–2 cases per million), the result of autosomal-recessively inherited congenital ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13) deficiency (ADAMTS13 activity <10% of the normal), and associated with yet many unanswered questions. Until December 2017, the Hereditary TTP Registry had enrolled 123 confirmed hTTP patients. Their median age at disease onset was 4.5 years (range: 0–70) and at clinical diagnosis 16.7 years (range: 0–69), a difference that highlights the existing awareness gap in recognizing hTTP. The systematic collection of clinical data of individual patients revealed their substantial baseline comorbidities, as a consequence of recurring TTP episodes in the past. Most notable was the high proportion of patients having suffered from premature arterial thrombotic events, mainly transient ischemic attacks, ischemic strokes, and to a lesser extent myocardial infarctions. At 40 to 50 years of age and above, more than 50% of patients had suffered from at least one such event, and many had experienced arterial thrombotic events despite regular plasma infusions every 2 to 3 weeks that supplements the missing plasma ADAMTS13. The article by van Dorland et al. (Haematologica 2019;104(10):2107–2115) and the ongoing Hereditary TTP Registry cohort study were recognized with the Günter Landbeck Excellence Award at the 50th Hemophilia Symposium in Hamburg in November 2019, the reason to present the Hereditary TTP Registry in more detail here.
A Rapid Mercury Droplet Electrode Polarography Method for Determination of Blood Lead Level in Lead Poisoned People
Authors: Hosseini Golriz , Hanaie Jalal , Ostadi Ali , Valipor Javad , Hamidi Aliasghar , Ghanbarzadeh Saeed 
Published: November 2020

Abstract
Background Lead is a hazardous heavy metal, which causes many problems in the human body. Unfortunately, recent reports showed that smugglers and opium sellers add lead to drugs during the production procedure in order to increase its weight and cost.
Purpose The aim of this study was development of a rapid and accurate method for measurement of blood lead levels (BLL) in the oral and inhaled opiate abuser people.
Methods BLL in samples obtained from the oral and inhaled opium addicted patients referring to Sina Hospital in Tabriz, Iran, during 2017 was compared with healthy control group (N=15). The wet digestion method was used to prepare whole blood and Mercury Droplet Electrode Polarography (MDEP) method was utilized for measurement of the lead content of digested samples.
Results Results showed that there were significant differences between the BLL of samples obtained from oral (17.12±74.61 μg/dL, p<0.0003) and inhaled (19.33±2.257 μg/dL, p<0.0001) opium addicted groups in comparison with healthy control group (4.669±0.3367 μg/dL).
Conclusion Based on the results of this study it was observed that BLL in opium addicted people needs to be measured as soon as possible. Furthermore, screening of blood lead concentrations in opium-addicted people with a rapid and accurate MDEP method is very necessary and important.
Scandium Triflate Catalyzed Nazarov Cyclization of Arylvinyl Epoxides Derived from Alkoxides and Chloro(aryl)carbenes: A Facile Access to Resveratrol-Derived Natural Products
Authors: Sudhakar Gangarajula , Satish Nagam 
Published: November 2020

AbstractThe reaction of arylvinyl alkoxides with chloro(aryl)carbenes provided the corresponding arylvinyl epoxides that underwent Nazarov cyclization in a catalytic amount of scandium triflate, providing easy access to several highly substituted indenes, including some resveratrol-derived natural products.
Solutions for Unexpected Challenges Encountered when Integrating Research Genomics Results into the EHR
Authors: Rasmussen Luke V. , Hoell Christin , Smith Maureen E. , Chisholm Rex , Starren Justin , Aufox Sharon , Luo Yuan , Rasmussen-Torvik Laura J. 
Published: November 2020

Abstract
Background While there have been published reports detailing technical challenges of incorporating genetic test results into the electronic health record (EHR) with proposed solutions, less has been published about unanticipated sociotechnological or practical communication challenges involved in this process.
Objectives This study was aimed to describe unanticipated issues that arose returning genetic research results through the EHR as part of the National Human Genome Research Institute (NHGRI)-funded electronic Medical Records and Genomics (eMERGE) 3 consortium, and provide lessons learned for future implementations
Methods We sequenced 3,000 participants on a 109-gene panel and returned genetic results initially in person and/or by letter, with a later release directly into the EHR and patient portal.
Results When results were returned through the EHR, multiple participants expressed confusion and contacted the health system, resulting in our institution temporarily freezing our return of research results.
Discussion We determined the likely causes of this issue to be (1) the delay between enrollment and results return, (2) inability to personalize mass e-mail messages announcing new research test results in the EHR, (3) limited space for description of test results in the EHR, and (4) the requirement to list an ordering physician for research results in the EHR. For future return of results, we propose sending preparatory e-mails to participants, including screenshots of how they can expect to see their results presented in the EHR portal.
Conclusion We hope our lessons learned can provide helpful guidance to other sites implementing research genetic results into the EHR and can encourage EHR developers to incorporate greater flexibility in the future.
COVID-19 Pulmonary Failure and Extracorporeal Membrane Oxygenation: First Experience from Three European Extracorporeal Membrane Oxygenation Centers
Authors: Yankah Charles Abraham , Trimlett Richard , Sandoval Elena , Lotz Christopher , Ledot Stephane , Pomar Jose Luis , Price Susanna , Meybohm Patrick 
Published: November 2020

AbstractOn April 17, 2020, a coronavirus disease 2019 (COVID-19) webinar was held by selected international experts in the field of intensive care and specialized respiratory ECMO centers from Germany, Italy, Spain, and the United Kingdom, which was hosted by the German Heart Centre Berlin/Charité. The experts shared their experience about the treatment of 42 patients with severe acute respiratory failure requiring venovenous extracorporeal membrane oxygenation (VV-ECMO). Patients were predominantly male (male-to-female ratio: 3:1), with a mean age of 51 years (range: 25–73 years). VV-ECMO support was indicated in 30% of the ventilated COVID-19 patients. The mean time requiring mechanical ventilation was 16.5 days, with a mean duration of ECMO support of 10.6 days. At the time of the webinar, a total of 17 patients had already been decannulated from ECMO, whereas six died with multiorgan failure. 18 patients remained on ECMO, with their final outcomes unknown at the time of the webinar. Hospital mortality was 25.6% (as of April 17, 2020). In this respect, VV-ECMO, provided by expert centers, is a recognized and validated mode of advanced life-support during the recent COVID-19 pandemic with good outcomes.
Hemorrhage in Essential Thrombocythemia or Polycythemia Vera: Epidemiology, Location, Risk Factors, and Lessons Learned from the Literature
Authors: Nicol Christophe , Lacut Karine , Pan-Petesch Brigitte , Lippert Eric , Ianotto Jean-Christophe 
Published: November 2020

AbstractHemorrhage is a well-known complication of essential thrombocythemia (ET) and polycythemia vera (PV), but evidence-based data on its management and prevention are lacking to help inform clinicians. In this review, appropriate published data from the past 15 years regarding bleeding epidemiology, classification, location, and risk factors are presented and discussed. Research was conducted using the Medline database. The bleeding classifications were heterogeneous among the collected studies. The median incidences of bleeding and major bleeding were 4.6 and 0.79% patients/year, in ET patients and 6.5 and 1.05% patients/year in PV patients, respectively. The most frequent location was the gastrointestinal tract. Bleeding accounted for up to 13.7% of deaths, and cerebral bleeding was the main cause of lethal hemorrhage. Thirty-nine potential risk factors were analyzed at least once, but the results were discrepant. Among them, age >60 years, bleeding history, splenomegaly, myeloproliferative neoplasm subtype, and platelet count should deserve more attention in future studies. Among the treatments, aspirin seemed to be problematic for young patients with ET (especially CALR-mutated ET patients) and anagrelide was also identified as a bleeding inducer, especially when associated with aspirin. Future studies should analyze bleeding risk factors in more homogeneous populations and with common bleeding classifications. More tools are needed to help clinicians manage the increased risk of potentially lethal bleeding events in these diseases.
Critical Galton-Watson branching processes with a countable set of types and infinite second moments
Authors: Vatutin V. A. , Dyakonova E. E. , Topchii V. A. 
Published: November 2020

Abstract
We consider an indecomposable Galton-Watson branching process with a countable set of types. Assuming that the process is critical and may have infinite variance of the offspring sizes of some (or all) types of particles we describe the asymptotic behaviour of the survival probability of the process and establish a Yaglom-type conditional limit theorem for the infinite-dimensional vector of the number of particles of all types.
Bibliography: 20 titles.
Polyhomomorphisms of locally compact groups
Authors: Neretin Yu. A. 
Published: November 2020

Abstract
Let

and

be locally compact groups with fixed two-sided invariant Haar measures. A polyhomomorphism

is a closed subgroup

with fixed Haar measure, whose marginals on

and

are dominated by the Haar measures on

and

. A polyhomomorphism can be regarded as a multi-valued map sending points to sets equipped with ‘uniform’ measures. For two polyhomomorphisms

and

there is a well-defined product

. The set of polyhomomorphisms

is a metrizable compact space with respect to the Chabauty-Bourbaki topology and the product is separately continuous. A polyhomomorphism

determines a canonical operator

, which is a partial isometry up to a scalar factor. For example, we consider locally compact linear spaces over finite fields and examine the closures of groups of linear operators in semigroups of polyhomomorphisms.
Bibliography: 40 titles.
Approximation of resolvents in homogenization of fourth-order elliptic operators
Authors: Pastukhova S. E. 
Published: November 2020

Abstract
We study the homogenization of a fourth-order divergent elliptic operator

with rapidly oscillating

-periodic coefficients, where

is a small parameter. The homogenized operator

is of the same type and has constant coefficients. We apply Zhikov’s shift method to obtain an estimate in the

-operator norm of order

for the difference of the resolvents

and

.
Bibliography: 25 titles.
Topological analysis of a billiard bounded by confocal quadrics in a potential field
Authors: Pustovoitov S. E. 
Published: November 2020

Abstract
Consider a billiard in a plane domain bounded by confocal ellipses and hyperbolae. A Hooke potential acts on a point mass. This dynamical systems turns out to be completely Liouville integrable. A topological analysis of the Liouville foliation of isoenergy manifolds at all possible levels of the Hamiltonian is performed and the complete Fomenko- Zieschang invariants (marked molecules) of these manifolds are constructed.	Bibliography: 15 titles.
Measurement of astrophysical S-factor for 9Be(d, α
0)7Li and 9Be(d, α
1)7Li* reactions at low energies *
Authors: Huang Zhenglin , Chen Bingjun , Qin Lixin , Zhang Qian , Zhang Fang , Wang Tieshan , Fang Kaihong 
Published: November 2020

Abstract
The thick-target yield of the 9Be(d, α
0)7Li and 9Be(d, α
1)7Li* reactions has been first directly measured over deuteron energies from 66 to 94 keV. The obtained S(E
i) of α
0 and α
1 have similar trends calculated by the thin-target yield, consistent with Yan’s report within the errors. Furthermore, the parametric expression of S(E) was obtained to calculate the theoretical thick target yield, and it roughly agrees with the experimental thick target yield.
Reduction of well stimulation period after hydraulic fracturing
Authors: Verisokin A E , Gun’kina T A , Vasil’yev V A , Shchekin A I , Serdyukov D Yu 
Published: November 2020

Abstract
This article discusses the problems associated with the removal of proppant, mechanical particles of the rock and undecomposed fluid from the hydraulic fracture into the well. The necessity of developing a device for washing the perforation interval has been identified and justified. It is shown that hardened and viscoelastic gel particles fall into perforations and adhere to the walls of channels and wells. Subsequently, these zones of gel deposition reduce permeability in perforation channels. The author has developed the design of a device for decolmation (declogging), which reduces the time for well development after hydraulic fracturing. To increase the productivity of the considered wells, it is necessary to flush them with the presented device in the mode of maximum approximation to deposits. To carry particles better to the surface, it is proposed to use foam systems generated by the jet pump, which is described in this article.
Method for improving the liquid hydrocarbon preparation efficiency
Authors: Kulikov A M , Gladenko A A , Alexandrov M A 
Published: November 2020

Abstract
According to the “Energy Strategy of Russia for the Period until 2030” and Decree of the Government of the Russian Federation of November 13, 2009 No. 1715-r, oil and gas companies have to use machines and equipment in the production process, as well as methods and processes that minimize energy consumption, and eliminate negative impacts of technological processes on the environment. New methods for the design, construction and operation of oil and gas facilities and improvement of the existing equipment should be developed and implemented.
The deployment of the collapsible piping through a large water obstacle
Authors: Melnikov D I , Seoev L V , Zemenkova M Yu , Podorozhnikov S Yu 
Published: November 2020

Abstract
Portable pipelines have been used in the Armed Forces of our country since the early 1960s to transfer light oil products. They are used to pump light oil products in large volumes for considerable distances in any climatic and geographic conditions. Portable pipelines were successfully used to eliminate natural and technogenic emergencies. The paper presents an overview of the deployment of portable pipelines across water barriers. It shows the organization and technology of works during the deployment of a pipeline across the 2700 m barrier of the Volga River.
Calculation of the torque on the crank shaft of the low-speed balancing drive sucker rod pumps
Authors: Galeev A.S. , Arslanov R.I. , Suleymanov R.N. , Filimonov O.V. 
Published: November 2020

Annotation
The operation of well sucker rod pump installations is the most massive way of mechanized oil production. A significant part of the wells equipped with low-speed plants of this type have a low flow rate and high water cut. Such conditions create certain restrictions on updating and modernization of the production fund of equipment in operation. The life cycle of a rocking machine is usually significant and often exceeds 20 years. During ISPR operation, both ground and downhole equipment will inevitably wear out. A significant proportion of failures of the ground part of low-speed ISPR is associated with the reduction of the rocking machine, which is the most responsible and most expensive drive element. The resource of the gearbox directly depends on its load, which is characterized by the moment on its crank (output) shaft. Reducing the maximum torque and moment fluctuations in one full swing of the rocking machine will significantly increase the reliability of the gearbox and the entire installation. This paper describes the mathematical apparatus for calculating the moment on the crank shaft of the gearbox, based on the exact kinematics of the drive and the principle of possible movements. The proposed methodology will allow to solve the problems of designing a balancing drive of a low-speed ISPR, to determine the requirements for the engine and transmission.
Development of a hydraulic classifier used to obtain various sand fractions
Authors: Verisokin A E , Yanukyan A P , Sorokin P M 
Published: November 2020

Abstract
The principle of crack filler selection depending on the crack depth and closing stress is presented. The study of existing classifiers and proposed a more effective device for sand sieving. The main elements of the classifier are presented and the principle of its operation is described.
The use of a liquid destructor with flow into the stream during the injection of the fracturing mixture
Authors: Fattakhov I G , Kuleshova L S , Kochetkov AV , Galiullina I F , Andaryanov I G , Safiullina A R , Gizatullin I R 
Published: November 2020

Abstract
For most modern development systems, hydraulic fracturing is the main method of intensification and maintenance of hydrocarbon production. Hydraulic fracturing is achieved with the volume of communicating voids between the granules of fractional proppant. The volume of voids should be maximized, which is achieved by selecting the optimal proppant fraction composition, and in addition, hydraulic fracturing fluid, the choice of which is based on a number of competing factors: high viscosity, which provides the necessary hydraulic width created at the buffer stage of the crack, as well as prediction of subsidence proppant in the fracture and subsequent premature stop of the injection, which creates additional costs. One of the new solutions, which will increase the efficiency and expand potential objects for hydraulic fracturing, is the use of a liquid destructor with a flow into the stream during the injection of the fracturing mixture.
Improving the design of a downhole filter for fluid production with an increased concentration of suspended particles
Authors: Khabibullin M Ya , Suleimanov R I , Mingulov Sh G , Kotenev Yu A , Sultanov Sh Kh 
Published: November 2020

Abstract
The reliability of the underground equipment of a well sucker rod pump installation will be improved by the use of a new sand filter design, the principle of which is based on the use of centrifugal force of a moving reservoir fluid. To determine the subtlety of cleaning the reservoir fluid from mechanical particles, it should be considered that the fluid velocity is the same as the particle velocity. To do this, we use the universal expression by analogy to our case for the stream function. To develop a specific filter (determination of structural dimensions) taking into account the well conditions, nomograms are developed taking into account all technical and technological conditions. The main advantage of the developed sand filter over other designs is the ability to pass formation fluid even when the cavity for collecting sand and the space between the filter housing and the pipe with radial channels are filled. The presence of the latter also does not affect the performance of the sucker rod pump, in the absence of jamming of the plunger in the cylinder.
Improving the method and equipment of thermo-acidic pulsation treatment
Authors: Khabibullin M Ya , Dubinskiy G S , Malyshev P M , Safiullina A R , Karimova A A 
Published: November 2020

Abstract
Initially, the method of thermo-acid pulsation was intended for heavily drained wells. But, as practice has shown, it can also be successfully used in the processing of wells of other categories, and depending on the diameter of the production string and the diameter of the reactor (jacket), the acid flow can be changed. A reactor with a diameter of 114 mm is usually used, but for 114 or 127 mm of the production string, a reactor with a diameter of 48 or 88 mm, respectively elongated, can be used to maintain the required capacity in order to accommodate the right amount of magnesium chips. The efficiency of acid treatment operations by pulsing is greater than the efficiency of thermo-acid treatments according to the results of calculations of both the increase in oil production and the duration of the wells at increased production.