bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Multi-Drug Resistant Mycobacterium Tuberculosis in Khyber Pakhtunkhwa – A High Tuberculosis Endemic Area of Pakistan
Authors: ALI SAJID , KHAN MUHAMMAD TAHIR , KHAN ANWAR SHEED , MOHAMMAD NOOR , KHAN MUHAMMAD MUMTAZ , AHMAD SAJJAD , NOOR SADIQ , JABBAR ABDUL , DAIRE CANTILLON , HASSAN FARIHA 
Published: June 2020

Abstract
Anti-tuberculosis therapy involves the combination of drugs to hamper the growth of Mycobacterium tuberculosis (MTB). The emergence of multidrug-resistant tuberculosis (MDR-TB) is a global concern. Pakistan has been ranked 5th position in terms of a high burden of MDR-TB in the world. The aim of the current study was to investigate the prevalence of drug resistance in MTB in Khyber Pakhtunkhwa. Random samples were collected from 25 districts using the simple random sampling formula. All samples were processed in a biosafety level 3 laboratory for culture and drug susceptibility testing. Among 5759 presumptive tuberculosis (TB) cases, 1969 (34%) were positive. The proportion of TB was higher in females (39%) than males (29%), thus it represents a significant association between gender and tuberculosis (p < 0.05). People ages between 25 to 34 years were more likely to be infected with MTB (40%). Drug-resistant profile showed 97 (4.9%) patients were infected with MDR-TB. Streptomycin resistance was the highest and was observed in 173 (9%) isolates followed by isoniazid in 119 (6%) isolates. The lowest resistance was observed to pyrazinamide (3%). The prevalence of MDR-TB (10.4%) among patients that previously received anti-tuberculosis treatment is seemingly high. A large-scale drug resistance survey is required to evaluate the drug resistance for better management of tuberculosis.
The Amelioration of Post Tin Mining Sand Tailing Medium with Chicken Manure for Pepper Cultivation
Authors: Inonu Ismed , Kusmiadi Riwan , Yuliana Ayu , Nurtjahya Eddy 
Published: June 2020

Inonu I, Kusmiadi R, Yuliana A, Nurtjahya E. 2020. The amelioration of post tin mining sand tailing medium with chicken manure for pepper cultivation. Jurnal Lahan Suboptimal: Journal of Suboptimal Lands 9(1): 31-40. Pepper cultivation in tin mining sand tailings requires the amelioration of organic matter to improve soil characteristics. The paper investigates the effect of chicken manure dosage on the growth of three pepper cultivars in sand tailing medium. This study was conducted on April-August 2016 at Experiment Station of Universitas Bangka Belitung, Balunijuk Village, Bangka Island. This study was arranged in a completely randomized design with two factors and three replications. The first factor was the dosage of chicken manure (5 kg; 6 kg; 7 kg/plant). The second factor was pepper cultivars (Lampung Daun Lebar; Lampung Daun Kecil; Merapin). The. result of the analysis of variance showed that the treatment of chicken manure dosage significantly affected only on total leaf area. The treatment of pepper cultivars significantly affected the total of chlorophyll, root dry weight, and shoot dry weight. There was no interaction between chicken manure dosage and pepper cultivar. Pepper cv. Lampung Daun Lebar tends to grow better than the other cultivars in the sand tailing medium.
Method of Estimating the Equivalence of Dissolution Profiles: a Modern View (Review)
Authors: Shohin I. E. , Bagaeva N. S. , Malashenko E. A. , Kuzina V. N. 
Published: May 2020

Introduction. One of the purposes of dissolution profile comparison is to establish the equivalence of dissolution profiles of the studied drug and the comparison drug.Text. According to the current regulatory documents, the main tool for quantitative confirmation of equivalence of drug release profiles is the calculation of the similarity factor (f 2). However, it does not consider the form of dissolution profiles, incomplete release of the drug substance, time correlation, and is not susceptible to the «outliers», which leads to false positive results. Special attention should be paid to the dissolution of drugs with high variability, which is not eliminated by either increasing the sample or changing the sampling scheme. If f 2 is not used, it is necessary to use model-dependent and model-independent methods that are statistically correct, and their use is sufficiently justified (difference factor f 1 , Weibull distribution function, comparison of release degrees at different time points (according to the student's t-criterion). However, these models have an empirical nature that calls into question the application of such methods. Multivariate analysis is widely discussed in the literature and can be used to compare the similarity of dissolution with the assumption that the data has a normal distribution. The most common methods for checking similarity of dissolution profiles for highly variable drugs are the Mahalanobis distance test and the bootstrap for f 2. There is a document of EMA about suitability of the Mahalanobis distance as a tool to assess the comparability of drug dissolution profiles and to a larger extent to emphasise the importance of confidence intervals to quantify the uncertainty around the point estimate of the chosen metric. The bootstrap methodology for f 2 does not provide a clear understanding of the application to dissolution profile comparison for incomplete-release drugs, particularly in biorelevant environments. The «T2EQ» function, based on the Mahalanobis distance for highly variable drugs (Hoffelder), gives undefined results in practice.Conclusion. The topic of equivalence of dissolution profiles requires discussion, since it is shown that the convergence factor is outdated and cannot be adequately applied. The use of modern methods does not have a clear regulatory confirmation by the regulatory authority. In the published scientific literature, several statistical methods have been explored and compared for their design and performance. It is necessary to develop a clear plan (decision treeы) for conducting the procedure for equivalence of dissolution profiles, employing a range of statistical methods.
The Influence of Deuterium on the Properties of Pharmaceutical Substances (Review)
Authors: Syroeshkin A. V. , Elizarova T. E. , Pleteneva T. V. , Uspenskaya E. V. , Levitskaya O. V. , Zlatskiy I. A. , Maksimova T. V. 
Published: May 2020

Introduction. The study discusses the hydrogen isotope 2 H effect on the biological activity of pharmaceutical substances.Text. Two aspects of the deuterium effect on the properties of active pharmaceutical ingredients and excipients are considered. The first one involves the use of deuterated substances, new compounds or substituted counterparts. Replacing protium with deuterium is used to reduce the rate of biotransformation. The kinetic isotope effect (KIE), expressed in a decrease in the rate of biotransformation as a result of deuteration, allows us to predict the rapid development of new directions in the development of pharmaceuticals. With the same therapeutic effect, an improvement in pharmacokinetic characteristics, a decrease in toxicity, a blocking of the epimerization of optically active substances, a change in the mechanisms of action are observed. The second aspect of the deuterium effect is associated with an increase in KIE of known pharmaceutical substances in aqueous solutions with a deuterium/protium ratio (D/H) lower than in natural water. For the first time, dose-response diagrams for deuterium demonstrate identity with essential microelements. There is a safety zone for the certain D/H relationship, beyond which the organism's vitality decreases. Improved kinetic characteristics are demonstrated for molecular level and for biological objects of various hierarchical levels. In particular, they include the possibility of increasing the dissolution rate of substances, the influence on the processes of mutarotation and the optical activity of chiral substances, the degree of accumulation of necessary elements in medicinal plants, and other processes.Conclusion. The results make it possible to predict the mechanisms of deuterium influence on the biochemical transformations of pharmaceutical substances in the body.
The Prospect of Creating Medicines Based on Selenium Nanoparticles (Review)
Authors: Skorinova K. D. , Kuzmenko V. V. , Vasilenko I. A. 
Published: May 2020

Introduction. The prospects of using nanoparticles in the production of medicines are widely discussed in the literature. In 2018 alone, the quantity of registration certificates issued by national regulators for medicines that use nanoparticles in one form or another is around forty. Most of them are medicines based on liposomes, polymers, iron oxides, micelles. So far, no registration certificates have been issued for selenium nanoparticles. One of the reasons for this situation in this area, from our point of view, is that the mechanisms of interaction of nanoparticles with cells are not sufficiently studied. The lack of basic research in this area is one of the main obstacles to the development of new-generation drugs based on nanoparticles.Text. This review is devoted to the analysis of scientific data on the interaction of selenium nanoparticles with different types of cells. The article discusses the biological properties of selenium and its role in cell metabolism. Data on the cytotoxic effect of selenium nanoparticles on various cell cultures are presented. Methods of preparation of nanoparticles and methods for studying the interaction of nanoparticles with cell cultures are described.Conclusion. Analysis of the literature data allows us to draw conclusions about the relevance of research on the interaction of selenium nanoparticles with living cells. This is necessary to determine the mechanisms of selenium nanoparticles absorption, study their cytotoxic and / or cytostatic action, and distribution in cells. Investigation of the biological interaction of selenium nanoparticles with tumor and normal cells will determine the most informative methods for registering and quantifying their antitumor activity, which is relevant for the development of new drugs to treat cancer.
Features of Hyaluronic Acid Solutions for Intra-articular Introduction and Recent Trends in Their Development (Review)
Authors: Zagorulko Y. Y. , Zagorulko E. Y. 
Published: May 2020

Introduction. The most common way to maintain the viscoelastic properties of synovial fluid is intra-articular administration of hyaluronic acid solutions. Such forms have several features due to the method of administration, the characteristics of the substance, as well as their composition, technology, and packaging. The aim of the work to analyze the features of hyaluronic acid solutions for intra-articular administration, as well as to consider resent trends to their pharmaceutical development.Text. Currently, in Russia, most of these forms are registered as medical devices. Each drug has its characteristics, including the source of the substance, the main molecular weight and the molecular weight range of hyaluronic acid, the structure of the molecule (linear or cross-linked), the method of its chemical modification, concentration, solution volume, dosage, etc. As excipients most often use sodium chloride, water for injection, and phosphate-buffered saline to maintain pH values close to the synovial fluid. Some prostheses contain mannitol as an antioxidant. Combinations of hyaluronic acid with active chondroprotective substances (chondroitin sulfate, sodium succinate) are known. The main type of primary packaging is glass prefilled syringes. The choice of sterilization methods is determined by the chemical structure of hyaluronic acid, aseptic production is used for most prostheses.Conclusion. Currently, research solutions to create thermostable and enzyme-resistant compositions with hyaluronic acid for intra-articular administration are being successfully applied. Modern developments are aimed at creating polymer complexes of hyaluronic acid with substances that improve the lubricity of solutions, the development of nanosystems (liposomes, nanoparticles, nano micelles, etc.) with chondroprotective, as well as the creation of inert biocompatible prostheses with viscoelastic properties. The creation of forms of hyaluronic acid and alternative drugs that can support the rheological properties of synovial fluid is currently a promising area of research.
Modern Technologies of Controlled Release of Biologically Active Substances in Pharmaceutical Research and Development (Review)
Authors: Savelieva E. I. 
Published: May 2020

Introduction. The review describes various systems used as inclusion matrices or modifiers of biologically active substances to enhance their absorption or deposition and subsequent release, both continuous or «on demand», i.e. in response to a stimulus.Text. Technologies for the incorporation of active substances into cyclodextrin nanoaggregates are developed to the greatest extent. Such technologies were used to obtain modified forms of hydrocortisone, glibenclamide, and a number of peptide drugs. Acetylcysteine immobilized on ethyl cellulose or other polymer particles significantly increases the bioavailability of peptide drugs on their intranasal administration. The deposition of active substances in the body takes place by way of their delayed controlled dissolution, adsorption, encapsulation, or esterification. The release of deposited substances upon exposure to an endogenous (change in pH, temperature) or external (exposure to ultrasound, electric or magnetic field, chemical activators) stimulus can be single or multiple, depending on the ability of the accommodating matrix for self-aggregation.Conclusion. Self-aggregated peptides are most promising for stimulus-induced release/delivery of biologically active substances. Modern technologies for the modification of active substances increase the efficiency of their administration and favor targeted location and implementation time of biological effects.
Interpolyelectrolyte Complexes Based on Eudragit® Copolymers as Carriers for Bioadhesive Gastroretentive Metronidazole Delivery System
Authors: Gordeeva D. S. , Sitenkova (Bukhovets) A. V. , Moustafine R. I. 
Published: May 2020

Introduction. There are a number of drugs, the absorption zone of which is the upper region of the gastrointestinal tract (GIT) – the stomach and duodenum. To increase bioavailability, gastroretentive (intragastric) systems for the controlled drug delivery are being developed. To date, there are various approaches to ensure intragastric drug delivery. One of the most promising approaches is the use of excipients with bioadhesive properties, both individually and in combination with other types of gastroretentive systems.Aim. Development and research of new carriers for gastroretentive bioadhesive drug delivery systems based on interpolyelectrolyte complexes (IPEC) with the participation of chemically complementary poly(meth)acrylates of the Eudragit®.Materials and methods. The study of swelling ability was carried out in a medium of 0.1 M hydrochloric acid solution (pH 1.2) at a temperature of 37 ± 0.5 °C for 6 hours. The study of the release of metronidazole (MZ) from matrices based on the corresponding IPEC was performed on a DFZ II instrument (ERWEKA, Germany) according to the Flow Trough Cell method in 0.1 M HCl medium, pH 1.2, flow rate 4 ml/min in a closed cycle within 6 hours. The amount of released MZ was estimated by UV spectrophotometry on a Lambda 25 instrument (PerkinElmer, USA) at a wavelength of 274 nm. IPEC adhesion was studied using a TA.XTplus texture analyzer (Stable Micro Systems, UK).Results and discussion. Matrices based on IPEC 1 were disintegrated after being in a medium with a pH of 1.2 for 4 hours, matrices based on IPEC 4 were dissolved in an acidic medium for 3 hours. At the same time, matrices based on IPEC 2 and IPEC 3 retain their shape throughout the experiment and are characterized by rather high values of the degree of swelling. IPEC samples are characterized by higher adhesion performance compared to individual copolymers. The release of metronidazole from matrices based on IPEC 1 occurs in accordance with Fick's law of diffusion; from the matrix based on IPEC 4, MZ is released according to the anomalous transport mechanism.Conclusion. IPEC 3 is promising for use as carrier for gastroretentive bioadhesive systems of controlled delivery of metronidazole.
Comparative Characteristics of Sugar Determination by Different Methods in Leaves of Nettle
Authors: Trineeva O. V. 
Published: May 2020

Introduction. According to the literature, nettle leaves contain polysaccharides and mucus as an integral part of the hydrophilic fraction. This group of biologically active substances (BAS) is involved in the manifestation of the physiological activity of the infusion. The traditional gravimetric method for the determination of polysaccharides in medicinal plant raw materials (PRM), pharmaceutical substances of plant origin and medicinal herbal preparations cannot provide a true picture of their content due to the presence of impurities. Therefore, it is advisable to determine the reducing sugars that are most fully extracted into the aqueous phase during the preparation of the infusion using modern physicochemical methods of analysis.Aim. The aim of the work was to determine the amount of polysaccharides and simple sugars in nettle leaves by various physico-chemical methods and their comparative characteristics.Materials and methods. A comparative determination of the sum of polysaccharides and simple sugars in the leaves of nettle by pharmacopoeial methods (picrine, anthrone and orcine) was carried out. Free and bound simple sugars were identified and quantified by thin layer chromatography (TLC). The composition and content of free simple sugars was studied by capillary electrophoresis (CE) in raw materials.Results and discussion. The content of free and bound sugars in raw materials has been established. The largest content of both the sum of polysaccharides and free simple sugars in the polysaccharide complex of nettle leaves, as well as the sum of free reducing simple sugars, according to experimental data, was obtained using the picrine method. The content of pentoses in the studied PRM was four times less, which is consistent with the literature data on the preferential construction of polysaccharides of plant objects from sugars of the hexose class. The resulting total content of the fraction of free sugars in the leaves, determined by the CE method, is an order of magnitude less compared with spectrophotometric methods. The obtained results of quantitative determination of the amount of free and bound simple sugars in the studied PRM are consistent with the determination data by the picrine pharmacopoeial method.Conclusion. In general, complete information, depending on the goals of the analysis, on the composition and quantitative content of simple and related sugars in the polysaccharide complex of plant objects can only be obtained by combining spectral methods with CE or TLC.
EXTENT OF HEMODIALYSIS NURSES’ PRESENCE AS PERCEIVED BY PATIENTS
Authors: Nebres Ma. Almira Pliego , Nebres Clarence Bien Ladion , Nebres Bienvisa Ladion 
Published: June 2020

Background: Nursing presence is an essential nurse caring behavior of being with the patient. This is particularly true for those with chronic renal disease and who regularly undergo maintenance hemodialysis treatment in the hospital setting. When nursing presence is perceived by patients in a nurse-patient relationship, it is more likely to result in enhanced satisfaction with the quality of nursing care. Thus, it is important for hemodialysis nurses to be aware of the relevance of nurse-patient interactions in planned care considering that these interactions are integral to the extent to which nursing presence is experienced and perceived by patients.Objective: This study aims to determine the hemodialysis nurses’ extent of nursing presence as perceived by patients undergoing hemodialysis treatment in two hospitals in Iligan City, Philippines.Methods: Utilizing the descriptive research design, the study was conducted in two hospitals in Iligan City, Philippines. A total of 121 hemodialysis patients were purposely selected to determine their perception as to the extent of hemodialysis nurse’s nursing presence utilizing the 25-item Presence of Nursing Scale (PONS) instrument. Descriptive statistics were used for data analysis.Result: The overall rating by the hemodialysis patients in both hospitals was “always" on the hemodialysis nurses’ extent of nursing presence with an average mean score of 4.47 in all 25 items of the PONS. The highest rated item which reflects that the nurse cares for the patient as a person had an average mean score of 4.63 (always) while the lowest rated item indicating that the nurse is meeting the spiritual needs of the patient had an average mean score of 4.23 (always).Conclusion: The nurse-patient relationship is enhanced by the presence of nurses who are more responsive and interactive with the patients, resulting in patients who are more satisfied with nursing care. Thus, it is suggested that nurses in dealing with patients of varying backgrounds should consider their unique needs when giving information, explanations, health education and nursing care.
Pregnant Mother's Consumes Fe and Anemia Tablets in City Palu
Authors: Pont Anna Veronica , Lisnawati Lisnawati 
Published: May 2020

The World Health Organization (WHO) estimates that around 10% of live births experience complications from postpartum bleeding. The most frequent complication of postpartum bleeding is anemia. The purpose of this study was to determine the relationship of compliance of pregnant women consuming Fe tablets with anemia in the Talise Health Center Community in Palu City. This type of research was a cross-sectional design that involved first and second-semester pregnant women, and as many as 209 people. The number of samples was 34 people. Purposive sampling technique. Univariate and bivariate analysis (chi-square test). The results showed 92.3% of respondents' compliance and 7.7% of anemia, while non-compliant respondents took 37.5% of Fe tablets without anemia, and 62.5% of those who were not obedient and had anemia. Chi-square test results p = 0.004 (<0.05), there was an effect of compliance of pregnant women with anemia in Talise Health Center Community, Palu City. In conclusion, there is an influence of compliance of pregnant women with anemia at the Talise Health Center Community in Palu City. It is recommended to actively conduct counseling about the benefits of Fe tablets during the pregnancy.