bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphatidic acid metabolism regulates neuroendocrine secretion but is not under the direct control of lipins
Authors: Baneux Claire , Tanguy Emeline , Thahouly Tamou , Vitale Alessio , Chasserot‐Golaz Sylvette , Bader Marie‐France , Gasman Stéphane , Vitale Nicolas 
Published: January 2020

AbstractPhosphatidic acid (PA) produced by phospholipase D1 has been shown to contribute to secretory vesicle exocytosis in a large number of cell models. Among various hypotheses, PA may contribute to recruit and/or activate at the exocytotic site a set of proteins from the molecular machinery dedicated to secretion, but also directly influence membrane curvature thereby favoring membrane rearrangements required for membrane fusion. The release of informative molecules by regulated exocytosis is a tightly controlled process. It is thus expected that PA produced to trigger membrane fusion should be rapidly metabolized and converted in a lipid that does not present similar characteristics. PA‐phosphatases of the lipin family are possible candidates as they convert PA into diacylglycerol. We show here that lipin 1 and lipin 2 are expressed in neuroendocrine cells where they are cytosolic, but also partially associated with the endoplasmic reticulum. Silencing of lipin 1 or 2 did not affect significantly either basal or evoked secretion from PC12 cells, suggesting that it is unlikely that conversion of PA into a secondary lipid by lipins might represent a regulatory step in exocytosis in neurosecretory cells. However, in agreement with a model in which PA‐metabolism could contribute to prevent entering into exocytosis of additional secretory vesicles, ectopic expression of lipin1B‐GFP in bovine chromaffin cells reduced the number of exocytotic events as revealed by carbon fiber amperometry recording. Furthermore, individual spike parameters reflecting fusion pore dynamics were also modified by lipin1B‐GFP, suggesting that a tight control of PA levels represents an important regulatory step of the number and kinetic of exocytotic events.
Hemolytic specimens in complete blood cell count: Red cell parameters could be revised by plasma free hemoglobin
Authors: Peng Zhaoyang , Xiang Wenqing , Zhou Jianming , Cao Jiajia , Li Zhe , Gao Hui , Zhang Junfeng , Shen Hongqiang 
Published: January 2020

AbstractIntroductionHemolysis is the main cause of unqualified clinical samples. In this study, we established a method for detecting and evaluating hemolysis in whole blood test. We used a mathematical formula for correcting the influence of hemolysis on complete blood cell count (CBC) so as to avoid re‐venipuncture and obtain more accurate parameters of red blood cell detection, reduce the burden of patients, and improve the efficiency of diagnosis and treatment.MethodsHemolytic samples were selected and then corrected using the new formula. Plasma free hemoglobin (fHB) was used as the criterion to determine the degree of hemolysis; the uncertainty of measurement is acceptable as the limit value of deviation between the measured value and the revised value. Hemolysis simulation analysis in vitro and continuous monitoring of clinical patients were used to verify the correction effect.ResultsA total of 83 clinical samples with hemolysis were collected and analyzed; fHB 1.4 g/L was selected as the unacceptable value for clinical hemolysis detection. In hemolytic samples, the red blood cell parameters corrected by formula are significantly different from those uncorrected and had a good consistency with those before hemolysis.ConclusionThe results show that the hemolysis phenomenon of CBC has a significant impact on routine blood testing. By using the new formula, the influence of hemolysis on erythrocyte and related parameters can be quickly and easily corrected, thus avoiding venipuncture again for re‐examination, reducing diagnostic errors, and saving medical resources.
New insights into the structure and function of chemokine receptor:chemokine complexes from an experimental perspective
Authors: Gustavsson Martin 
Published: January 2020

Abstract
Chemokines are small soluble proteins that drive cell migration through the formation of concentration gradients. Chemokine binding to G protein-coupled chemokine receptors in the cell membrane activates intracellular signaling pathways and is a fundamental process involved in numerous physiological and pathophysiological functions. In the past few years, significant experimental developments have made it possible to characterize complexes between chemokine receptors and chemokines at a molecular level. Here, I review these developments from an experimental perspective, focusing on how the ability to express, purify, and stabilize receptor:chemokine complexes have made studies by X-ray crystallography, nuclear magnetic resonance, and other methods possible. I give examples of how these studies have advanced our understanding of the architecture of receptor:chemokine complexes as well as the mechanisms involved in complex formation. Finally, I discuss some of the many remaining questions and challenges that will require studies of more receptors and chemokines as well as further development of experimental methods.
Pitfalls in the characterization of circulating and tissue-resident human γδ T cells
Authors: Beucke Nicola , Wesch Daniela , Oberg Hans-Heinrich , Peters Christian , Bochem Jonas , Weide Benjamin , Garbe Claus , Pawelec Graham , Sebens Susanne , Röcken Christoph , Hashimoto Hisayoshi , Löffler Markus W. , Nocerino Paola , Kordasti Shahram , Kabelitz Dieter , Schilbach Karin , Wistuba-Hamprecht Kilian 
Published: January 2020

Abstract
Dissection of the role and function of human γδ T cells and their heterogeneous subsets in cancer, inflammation, and auto-immune diseases is a growing and dynamic research field of increasing interest to the scientific community. Therefore, harmonization and standardization of techniques for the characterization of peripheral and tissue-resident γδ T cells is crucial to facilitate comparability between published and emerging research. The application of commercially available reagents to classify γδ T cells, in particular the combination of multiple Abs, is not always trouble-free, posing major demands on researchers entering this field. Occasionally, even entire γδ T cell subsets may remain undetected when certain Abs are combined in flow cytometric analysis with multicolor Ab panels, or might be lost during cell isolation procedures. Here, based on the recent literature and our own experience, we provide an overview of methods commonly employed for the phenotypic and functional characterization of human γδ T cells including advanced polychromatic flow cytometry, mass cytometry, immunohistochemistry, and magnetic cell isolation. We highlight potential pitfalls and discuss how to circumvent these obstacles.
Expressing forgiveness after interpersonal mistreatment: Power and status of forgivers influence transgressors' relationship restoration efforts
Authors: Zheng Michelle Xue , van Dijke Marius 
Published: January 2020

SummaryOne adverse consequence of interpersonal mistreatment is that it damages the relationship between the victim and the transgressor. Scholars have promoted forgiveness of such mistreatment as a victim response that can motivate transgressors to work towards relationship restoration. Building on social exchange theory and the social perception literature, we provide an account of when transgressors are less (vs. more) willing to restore their relationship with the victim in response to forgiveness. Specifically, we argue that transgressors perceive forgiveness from a victim who has high (vs. low) power, relative to the transgressor, as insincere, making transgressors less willing to restore the relationship. We further argue that this effect of high (vs. low) victim power is pronounced especially when the victim also has low (vs. high) status. Two experiments and two field studies support these predictions. These findings highlight the relevance of studying how contextual conditions color transgressors' perceptions of victims' behavior to understand relationship restoration after interpersonal mistreatment.
Skilful two‐month‐leading hybrid climate prediction for winter temperature over China
Authors: Dai Haixia , Fan Ke 
Published: January 2020

AbstractTwo skilful 2‐month‐leading hybrid downscaling prediction schemes in October for winter surface air temperature (SAT) over China are proposed in this paper. The schemes are based on the year‐to‐year increment approach and the coupled climate patterns between the winter SAT over China and its predictors. Observed North Pacific sea surface temperature (SST) from the preceding July to September, Arctic sea ice concentration (SIC) in the preceding August and winter sea level pressure (SLP) over pan Eurasia from version 2 of the Climate Forecast System (CFSv2) are selected as the predictors based on the fundamental physics. Individual‐predictor schemes (IP‐schemes), that is, SLP‐scheme, SST‐scheme, SIC‐scheme, indicate that these predictors exhibit prediction skills in different regions. Multi‐predictor scheme I (MP‐schemeI) is developed by combining three predictors. However, MP‐schemeI shows limited skill in predicting SAT over Northeast China (NECTA), due to the limited skill of CFSv2 over the extratropics. Thus, MP‐schemeII is established, in which a hybrid downscaling model for NECTA is constructed. These two MP‐schemes have comparable prediction skill over China, but MP‐schemeII outperforms MP‐schemeI over NEC. The temporal (spatial) anomaly correlation coefficient (ACC) increases from 0.23 (0.15) in MP‐schemeI to 0.36 (0.21) in MP‐schemeII, and the ratio of the same sign of anomalous years (Anomalous‐RSS) improves from 39.1% to 56.5% over NEC. For the winter SAT over China, the MP‐schemes greatly enhance the prediction compared with CFSv2 outputs and the IP‐schemes. The temporal (spatial) ACC increases from 0.29 (0.05) in CFSv2 to 0.52 (0.29) in the MP‐schemes, and the station‐average root‐mean‐square error decreases by about 48.0% compared with CFSv2. Moreover, the RSS (Anomalous‐RSS) of the winter SAT over China is 55.9% (47.4%) in CFSv2 and 64.7% (63.2%) in the MP‐schemes. This indicates that the MP‐schemes perform better in predicting anomalous winters.
An algebraic alternative for the accurate simulation of CO2 Raman spectra
Authors: Bermúdez‐Montaña Marisol , Carvajal Miguel , Pérez‐Bernal Francisco , Lemus Renato 
Published: January 2020

AbstractWe present an accurate simulation of the Raman spectrum of the carbon dioxide molecule in the 1150–1500 cm−1 spectral range, comparing the results obtained using the three polyad schemes found in the literature of this molecule. The description of the molecule with the algebraic U1(2)×U(3)×U2(2) local model encompasses both stretching and bending degrees of freedom. A detailed analysis of the Hamiltonian interactions for the three polyad schemes provides fittings with root mean square deviations in the range 0.14–0.20 cm−1, involving 19 parameters taking into account the 178 experimental term energies found in the literature. Using a limited subset of 9 experimental transition moments, we optimize 5 partial derivatives of the mean polarizability and simulate the Raman spectrum of CO2 for the three polyad schemes. Comparing the calculated results with the experimental spectrum, we obtain an overall good agreement for the three polyads. However, an inspection in detail of the spectrum seems to show a slight preference for polyad P212 albeit not due to the interaction characterizing the polyad but due to anharmonic effects and energy distribution. Finally, we assess the effect of the Fermi resonance over CO2 Raman line intensities.
Micro‐by‐micro interactions: How microorganisms influence the fate of marine microplastics
Authors: Rogers Kelsey L. , Carreres‐Calabuig Joan A. , Gorokhova Elena , Posth Nicole R. 
Published: January 2020

AbstractMicroorganisms drive the biogeochemical cycles that link abiotic and biotic processes in the aqueous environment and are intricately associated with plastic debris. The presence of microplastics in water and sediment introduces new concerns as small particle size allows for increased pathways of microplastics in the food web and element cycles. In this review, we present the current state of knowledge on microbe‐plastic interactions and summarize the potential impact of biogeochemical processes on plastic distribution, cycling, transport, and sedimentation. We explore how microbe‐plastic interactions influence the exposure of consumers to microplastics and plastic degradation products. Key methods used to elucidate biofilm development, microbial biodegradation, and microplastic detection in the aqueous environment are discussed. Finally, we comment on potential future questions and research directions needed to further define the role of microorganisms in the environmental fate of microplastics.
NYX‐458 Improves Cognitive Performance in a Primate Parkinson's Disease Model
Authors: Barth A.L , Schneider J.S. , Johnston T.H. , Hill M.P. , Brotchie J.M. , Moskal J.R. , Cearley Cassia N. 
Published: January 2020

AbstractBACKGROUNDNYX‐458 is a N‐methyl‐d‐aspartate receptor (NMDAR) modulator that enhances synaptic plasticity. Dopaminergic cell loss in Parkinson's disease (PD) leads to NMDAR dysregulation in the cortico‐striato‐pallidal‐thalmo‐cortical network and altered plasticity in brain regions important to cognitive function. We hypothesize that targeting the NMDAR may be an efficacious approach to treating cognitive impairment in PD.OBJECTIVESNYX‐458 was evaluated in 2 nonhuman primate models of PD. The first, a chronic low‐dose 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)–administration model, was used to assess the effects of NYX‐458 on cognitive domains impacted early in PD including attention, working memory, executive function, and visuospatial learning. The second, a high‐dose MPTP‐administration model, was used to assess potential for NYX‐458 induced change in motor symptoms.METHODSNYX‐458 was evaluated in the chronic low‐dose MPTP model using the variable delayed response measure to assess attention and working memory and simple discrimination reversal to assess executive function. NYX‐458 was also assessed in the high‐dose MPTP model as a monotherapy and in combination with low‐dose or high‐dose levodopa to assess potential impact on motor symptoms.RESULTSNYX‐458 administration resulted in rapid and long‐lasting improvement in cognitive function across the domains of attention, working memory, and executive function. Dose levels effective in improving cognitive performance had no effect on PD motor symptoms, the antiparkinsonian benefit of levodopa, or dyskinesia.CONCLUSIONSNYX‐458 provides benefit in specific domains known to be impaired in PD in a dopamine depletion model of PD‐like cognitive impairment. These data support the continued evaluation of NYX‐458 as a potential therapeutic for cognitive decline in PD. © 2020 International Parkinson and Movement Disorder Society
Retracted: Resveratrol Derivative, Trans‐3, 5, 4′‐Trimethoxystilbene, Prevents the Developing of Atherosclerotic Lesions and Attenuates Cholesterol Accumulation in Macrophage Foam Cells
Authors: Hong Ming , Li Jinke , Li Siying , Almutairi Mohammed M. 
Published: January 2020

ScopeRecent studies have demonstrated that trans‐3, 5, 4′‐Trimethoxystilbene (TMS), a novel derivative of resveratrol, may suppress the foam cells formation and restrain atherosclerosis in vitro and in vivo. Herein, the molecular mechanisms underlying the protective effects of TMS against atherosclerosis are further delineated.Methods and ResultsIn the present study, the cholesterol‐lowering effects of TMS in macrophage‐derived foam cell by animal studies, Oil Red O staining, and lipid uptake as well as efflux analysis, are explored. Real‐time PCR, western blotting analysis, luciferase reporter assay, electrophoretic mobility shift assay, and immunofluorescent staining are applied for investigating the mechanism involved in atherosclerosis prevention by TMS. Herein, it is revealed that TMS, at a dosage of 10 mg kg−1 day−1, may suppress atherosclerotic plaques within the aorta and arterial intima in apolipoprotein Edeficient mice (ApoE)−/− mice by reducing cholesterol level and macrophages content. Exposure of macrophages to TMS (10 µM) can suppress foam cells formation via regulating oxidized low density lipoprotein and cholesterol content in human macrophages through inhibiting scavenger receptors expression and activator protein‐1(AP‐1) activity. In addition, TMS can activate ERK/Nrf2/HO‐1 signaling which increases the expression of ATP‐binding cassette transporters.ConclusionIn conclusion, TMS may inhibit the progress of atherosclerosis through regulating cholesterol homeostasis and inhibiting macrophage‐derived foam cells formation.
Compact filtering power divider based on corrugated third‐mode circular SIW cavities
Authors: Moznebi Ali‐Reza , Afrooz Kambiz , Danaeian Mostafa , Mousavi Pedram 
Published: January 2020

AbstractIn this article, a compact single‐layer filtering power divider based on third‐mode circular substrate integrated waveguide (SIW) cavities is proposed. The third‐mode SIW technique is introduced to divide an SIW cavity into three cavities and get 66% size reduction. The proposed power divider is composed of three third‐mode circular SIW cavities within a circuit with the size of a circular SIW cavity. The power is coupled from the input cavity to two output cavities through the electric coupling, separately. To achieve a more size reduction, the designed cavities are corrugated along the cutting planes. By increasing the length of the corrugated edges, the center frequency can be easily shifted to lower frequencies. In order to prove the proposed design procedure, the presented power divider with a second‐order bandpass filtering response is designed and fabricated. Reasonable agreements between the measured and simulated results are found. The measured results show that the power divider achieves an insertion loss of 1.2 dB at 5.61 GHz, return loss of higher than 14 dB, and 3‐dB fractional bandwidth of 7.8%. The results show that this power divider has the advantages of the compact size, high selectivity, and single‐layer structure.
K‐PSO: An improved PSO‐based container scheduling algorithm for big data applications
Authors: Liu Bo , Li Jiawei , Lin Weiwei , Bai Weihua , Li Pengfei , Gao Qian 
Published: January 2020

SummaryIn recent years, Docker container technology is being applied in the field of cloud computing at an explosive speed. The scheduling of Docker container resources has gradually become a research hotspot. Existing big data computing and storage platforms apply with traditional virtual machine technology, which often results in low resource utilization, a long time for flexible scaling and expanding clusters. In this paper, we propose an improved container scheduling algorithm for big data applications named Kubernetes‐based particle swarm optimization(K‐PSO). Experimental results show that the proposed K‐PSO algorithm converges faster than the basic PSO algorithm, and the running time of the algorithm is cut in about half. The K‐PSO container scheduling algorithm and algorithm experiment for big data applications are implemented in the Kubernetes container cloud system. Our experimental results show that the node resource utilization rate of the improved scheduling strategy based on K‐PSO algorithm is about 20% higher than that of the Kube‐scheduler default strategy, balanced QoS priority strategy, ESS strategy, and PSO strategy, while the average I/O performance and average computing performance of Hadoop cluster are not degraded.
Taboo and Descent in the Articulation of Gender Relations: An Eastern Indonesian Case
Authors: Forth Gregory 
Published: January 2020

ABSTRACTIn the Lio region of Flores Island patrilineal groups coexist with taboos on animals and plants inherited through females, a combination previously interpreted as reflecting a system of double unilineal descent. Drawing on ethnography from the Lio district of Mego, maternal taboos are shown to accompany similar prohibitions conceived as a property of patriclans, most of which, however, are incumbent not on male clansmen but on their wives. As is further demonstrated, both sorts of taboo reflect ideas about female bodies and blood, particularly as women and their children are transformed through marriage into members of patrilineal groups dominated by men. Also discussed is whether variability in the way taboos are currently inherited in Mego may reflect recent social change, and how matrilineally inherited Lio prohibitions can be seen as an instance of complementary female and male principles equally operative in the social life of other eastern Indonesian societies.
Association between shared medical appointments and weight loss outcomes and anti‐obesity medication use in patients with obesity
Authors: Shibuya Kelly , Ji Xinge , Pfoh Elizabeth R. , Milinovich Alex , Weng Wayne , Bauman Janine , Ganguly Rahul , Misra‐Hebert Anita D. , Hobbs Todd M. , Kattan Michael W. , Pantalone Kevin M. , Ramasamy Abhilasha , Burguera Bartolome 
Published: January 2020

SummaryObjectiveIn shared medical appointments (SMAs), multiple patients with a similar clinical diagnosis are seen by a multidisciplinary team for interactive group sessions. Very few studies have specifically studied SMAs and weight loss in patients with obesity. This study compared weight loss outcomes and anti‐obesity medication (AOM) access between patients with obesity managed through (SMAs) versus individual appointments.MethodsRetrospective study of adults seen for obesity between September 2014 and February 2017 at Cleveland Clinic Institute of Endocrinology and Metabolism. Percent weight loss from baseline was compared between two propensity score‐matched populations: patients who attended ≥1 SMA and patients managed with individual medical appointments.ResultsFrom all eligible patients identified (n=310 SMA, n=1,993 non‐SMA), 301 matched pairs were evaluated for weight loss. The SMA group (n=301) lost a mean of 4.2%, 5.2% and 3.8% of baseline weight over 6, 12 and 24 months; the non‐SMA group (n=301) lost significantly less weight (1.5%, 1.8% and 1.6%, respectively) (paired t‐test, P<.05). All patients were eligible for US Food and Drug Administration‐approved AOMs based on obesity diagnosis; however, 49.8% (150/301) of matched SMA patients were prescribed an AOM versus 12.3% (37/301) of matched non‐SMA patients.ConclusionThis study suggests that SMAs may offer a promising alterative for obesity management and one that may facilitate greater utilization of AOMs. In propensity score‐matched cohorts, SMAs were associated with greater weight loss outcomes when compared to usual care facilitated through individual medical appointments alone.