bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel Association Between FOXO3 rs2232365 Polymorphism and Late-Onset Preeclampsia: A Case-Control Candidate Genetic Study
Authors: Pan Feng Xue , Wei Jie Ben , Wang Hong , Ma Yu Ling , Du Li Shao , Chen Ying 
Published: September 2020

Abstract
BackgroundBoth genetic susceptibility and dysregulated lipid metabolism are important susceptibilities to preeclampsia. In the study, we devote to investigate the associations of FOXO3 and TLR7 genetic polymorphisms with preeclampsia in a Chinese population.MethodsThis case-control study involved 335 Han Chinese pregnant women, including 177 pregnant women with preeclampsia and 158 healthy controls. The preeclampsia group was further sub-grouped into early-onset preeclampsia (EOPE, n=70）and late-onset preeclampsia (LOPE, n=107）. Three single nucleotide polymorphisms SNPs , including FOXO3 rs2232365, rs3761548, and TLR7 rs3853839 were genotyped by multiplex PCR for targeted next-generation sequencing. The χ2 test and multiple interaction effect analyses were performed to determine the association of three SNPs with serum lipid levels and thyroid function in women with preeclampsia.ResultsOur study showed that SNP rs2232365 C allele frequencies were significantly associated with the occurrence of LOPE (P=0.022, odds ratio = 1.72 (0.95 CI: 1.23-2.41)). The genotype (CC vs TT+CT) distribution of rs2232365 revealed a significant association with LOPE (P=0.004, odds ratio = 3.497 (0.95 CI: 1.498-8.164)). No significant difference was found in the genotype and allele frequencies of rs3761548 and rs3853839 between controls and cases (P>0.05). Moreover, the genotype CC of rs2232365 was significantly correlated with increased TG/HDL levels in the LOPE group (all p=0.014). ConclusionsThe polymorphisms of rs2232365 are associated with the risk of LOPE and may modulate TG/HDL levels in pregnant women with LOPE.
Reduced Efficacy of a Src Kinase Inhibitor in Crowded Protein Solution
Authors: Kasahara Kento , Re Suyong , Nawrocki Grzegorz , Oshima Hiraku , Mishima-Tsumagari Chiemi , Kukimoto-Niino Mutsuko , Yu Isseki , Shirouzu Mikako , Feig Michael , Sugita Yuji 
Published: September 2020

Abstract
The inside of a cell is highly crowded with proteins and other biomolecules. How proteins express their specific functions together with many off-target proteins in crowded cellular environments is largely unknown. Here, we investigate an inhibitor binding with c-Src kinase using atomistic molecular dynamics (MD) simulations in dilute as well as crowded protein solution. The populations of the inhibitor, PP1, in bulk solution and on the surface of c-Src kinase are reduced as the concentration of crowder bovine serum albumins (BSAs) increases. This observation is consistent with the reduced PP1 inhibitor efficacy in experimental c-Src kinase assays in addition with BSAs. The crowded environment changes the major binding pathway of PP1 toward c-Src kinase compared to that in dilute solution. This change is explained based on the population shift mechanism of local conformations near the inhibitor binding site in c-Src kinase. Protein functions in a living cell could be examined using atomistic MD simulations with realistic cellular environments.
Uncovering the Mechanism of Ge-Gen-Qin-Lian Decoction for Treating Ulcerative Colitis Based on Network Pharmacology and Molecular Docking Verification
Authors: Xu Lin , Zhang Jiaqi , Zhang Zedan , Wang Yifan , Wang Fengyun , Tang Xudong 
Published: September 2020

Abstract
Background and objective: Ge-Gen-Qin-Lian Decoction (GGQLD), a traditional Chinese medicine (TCM) formula, has been widely used for ulcerative colitis (UC) in China while the pharmacological mechanisms still remain unclear. The present research was designed to clarify the underlying mechanism of GGQD against UC. Methods: In this research, a GGQLD-compound-target-UC (G-U) network was constructed based on public databases to clarify the relationship between active compounds in GGQLD and potential targets. GO and KEGG pathway enrichment analyses were performed to investigate biological functions associated with potential targets. A protein-protein interaction network was constructed to screen and evaluate hub genes and key active ingredients, another GO and KEGG pathway analyses were subsequently performed on hub genes. Molecular docking was used to verify the activities of binding between hub targets and ingredients. Results: Finally, 83 potential therapeutic targets and 118 correspond active ingredients were obtained by network pharmacology. GO and KEGG enrichment analysis revealed that GGQLD had an effect of anti-inflammation, antioxidation, and immunomodulatory. The effect of GGQLD on UC might be achieved by regulating the balance of cytokines (eg., IL6, TNF, IL1β, CXCL8, CCL2, IL10, IL4, IL2) in immune system and inflammation-related pathways, such as IL-17 pathway and Th17 cell differentiation pathway. Besides, molecular docking results demonstrated that the main active ingredients, quercetin, exhibited good affinity to hub targets. Conclusion: This research fully reflects the characteristics of multi-component and multi-target for GGQLD in the treatment of UC. Furthermore, the present study provided new insight into the mechanisms of GGQLD against UC.
Programming complex regulation mechanisms through simple molecular assembly
Authors: Lauzon Dominic , Vallee-Belisle Alexis 
Published: September 2020

Abstract
What are the advantages and disadvantages of building nanosystems using one or multiple components? More than 55% of all proteins found in living organisms are multimeric and likely exploit molecular assembly to create new functional entities. However, the specific contribution of molecular assembly to the creation of novel functions remains relatively unexplored at the thermodynamic, kinetic and molecular levels. Here, we use theory and a simple experimental model to determine the design rules for engineering efficient self-assembled, self-regulated nanosystems. Using these rules, we have rationally designed and implemented various regulation mechanisms (e.g., cooperative and anticooperative assembly, self-inhibition, molecular timer) into two model trimeric nanosystems including a complex artificial catalyst. These simple strategies based on molecular assembly have been extensively exploited by natural biosystems and are expected to play a crucial role in the development of future self-regulated nanotechnologies.
Investigating The Risk Factors for Hypertension in A Population-Based Study: Fasa PERSIAN COHORT Data
Authors: Bijani Mostafa , Parvizi Saeed , Dehghan Azizallah , Rahimabadi Massih Sedigh , Chijan Mahsa Rostami , Kazemi Maryam , Naghizadeh Mohammad Mehdi , Ghaemi Alireza , Homayounfar Reza , Farjam Mojtaba 
Published: September 2020

Abstract
Background: High prevalence of hypertension (HTN) and its subsequent serious complications make this disease as a major health-treatment concern in many societies. The current study aimed to investigate known factors as risk factors for hypertension and the way of their association in study population of Fasa cohort in south of Iran at 2019.Methods: This is an analytical-cross sectional study. Study population was the individuals covered by Fasa cohort. Information of the first phase of Fasa Persian cohort in south of Iran was used in the study. Independent t-test, chi-square test, analysis of variances, Pearson correlation coefficient, and logistic regression were used to analyze data. Data was analyzed using SPSS software version 22, and P-value<0.05 was considered statistically significant.Results: Out of the population of 10111 individuals of the study, 5546(54.86%) subjects were female and 4565(45.16%) were male. The overall prevalence of hypertension was 41.8 %. In the present study (28/5%) were with HTN stage 1, and (13/3%), were with HTN stage 2. In addition, a significant association was observed among HTN and cardiovascular diseases, diabetes and renal failure (p<0.5).Conclusions: prevalence of HTN in study population is considerable. Given its hazardous complications, application of appropriate methods in order to screen, prevent and treat HTN is necessary. In addition, using training programs, changing lifestyle, and promoting self-care behaviors can be effective in prevention, control, and decrease in hazardous complications caused by HTN.
Altered Behaviour Associated With Autism in a Mouse Model of Fragile X Syndrome Treated With Bacteroides Fragilis BF839
Authors: Lin Chu-Hui , Zeng Ting , Lin Jian-Hong , Xiao Feng , Li Bing-Mei , Chen Sheng-Qiang , Deng Yu-Hong 
Published: September 2020

Abstract
Background: Fragile X syndrome (FXS), tightly related to the morbidity of Autism spectrum disorder (ASD), is a common hereditary syndrome often associated with retardation of intelligence. Some key symptoms of ASD such as anxiety, cognitive impairment and social anxiety disorder are also the predominant features in FXS. Children with ASD are often performed with gastrointestinal symptoms. According to the existing research, with the treatment with Bacteroides Fragilis BF839, mice with ASD will have better performance in communication and social behaviours with less anxiety and perceptual disorder. In this article, we have observed the impact of Bacteroides Fragilis BF839, a well-established Chinese bacteria strain with the human intestine origin, on mice with FXS and their behavioural disorders accordingly. Result: Based on the Open Field test, compared to the Fmr1KO group, mice treated with BF839 showed prolonged staying time in the center of the container. This finding suggests that BF839 can improve Fmr1KO mice's self-exploration behaviour and dented their anxiety. The Elevated Plus Maze test indicated BF839 treated mice presented more activities in entering open arms, prolonged time of staying and significantly less distance travelled at the plus-maze, along with less entering behaviours in the closed arms with less time of staying and more distance travelled. This result proved that with the treatment of BF839, Fmr1KO mice have improved ability in recognizing the surrounding environment and greater senses at detecting danger. Three-box Social Interaction test confirmed that BF839 strengthens the social novelty preference of the Fmr1KO mice, proven by their increasing duration and frequency in social interacting with the stranger mouse. The final experiment named the Pool Maze test presented the result that on the fourth day, BF839 treated mice have shown significantly shortened escape latency. Meanwhile, on Day 5, BF839 treated group performed increasing frequency in passing through the platform, which, along with the shortened escape latency, demonstrated BF839 has the function of improving Fmr1KO mice's cognitive capacity and their ability to extract information from the surrounding environment.Conclusion: Based on the outcome of each test performed, Bacteroides Fragilis BF839 can successfully improve Autism related abnormal behaviours in mice with FXS. Bacteroides Fragilis BF839 can be a potential intervention strategy in treating FXS and ASD safely and effectively.
Successfully REBOA&nbsp;Performance: Does Medical Specialty Matter? International Data From the ABOTrauma&nbsp;Registry.
Authors: Hilbert-Carius Peter , McGreevy David , Abu-Zidan Fikri M , Hörer Tal 
Published: September 2020

Abstract
BackgroundResuscitative Endovascular Balloon Occlusion of the Aorta (REBOA) is a minimally invasive procedure being increasingly utilized to prevent patients with non-compressible torso hemorrhage from exsanguination. The increased use of REBOA is giving rise to discussion about “Who is and who should be performing it?”.MethodsData from the international ABO (Aortic Balloon Occlusion) Trauma registry from between November 2014 and April 2020 were analyzed concerning the question: By who, how and where is REBOA being performed? The registry collects retrospective and prospective data concerning use of REBOA in trauma patients.ResultsDuring the study period, 259 patients had been recorded in the registry, 72.5% (n=188) were males with a median (range) age of 46 (10-96) years. REBOA was performed in the ER in 50.5%, in the OR in 41.5% and in the angiography suite in 8% of patients. In 54% of the patients REBOA was performed by surgeons (trauma surgeons 28%, vascular surgeons 22%, general surgeons 4%) and in 46% of the patients by non-surgeons (emergency physicians 31%, radiologists 9.5%, anesthetists 5.5%). Common Femoral Artery (CFA) access was achieved by use of external anatomic landmarks and palpation alone in 119 patients (51%), by cut down in 57 patients (24%), using ultrasound in 49 patients (21%) and by fluoroscopy in 9 patients (4%). Significant differences between surgeons and non-surgeons were found regarding patient’s age, injury severity, access methods, place where REBOA was performed, location patients were taken to from the emergency room and mortality.ConclusionA substantial number of both surgical and non-surgical medical disciplines are successfully performing REBOA to an almost equal extent. Surgical cut down is used less frequently as access to the CFA compared with reports in older literature and puncture by use of external anatomic landmarks and palpation alone is used with a high rate of success. Instead of discussing “Who should be performing REBOA?” future research should focus on “Which patient benefits most from REBOA?”.
Breast Cancer Detection From a Urine Sample by Dog Sniffing
Authors: Kure Shoko , Iida Shinya , Yamada Marina , Takei Hiroyuki , Yamashita Naoyuki , Sato Yuji , Miyashita Masao 
Published: September 2020

Abstract
Background: Breast cancer is a leading cause of cancer death worldwide. Several studies have demonstrated that dog can sniff and detect cancer in the breath or urine sample of a patient. Objective: The aim of this study is to assess whether the trained dog can detect breast cancer from urine samples.Methods: A nine-year-old female Labrador Retriever was trained to identify cancer from urine samples of breast cancer patients. Urine samples from patients histologically diagnosed with primary breast cancer, those with non-breast malignant diseases, and healthy volunteers were obtained, and a double-blind test was performed. Results: 40 patients with breast cancer, 142 patients with non-breast malignant diseases, and 18 healthy volunteers were enrolled, and their urine samples were collected. In 40 times out of 40 runs of a double-blind test, the trained dog could correctly identify urine samples of breast cancer patients. Sensitivity and specificity of this breast cancer detection method using dog sniffing were both 100%.Conclusions: The trained dog in this study could accurately detect breast cancer from urine samples of breast cancer patients. These results indicate the feasibility of a method to detect breast cancer from urine samples using dog sniffing in the diagnosis of breast cancer.
Laboratory based Retrospective Study to determine the start of SARS-CoV-2 in Patients with Severe Acute Respiratory Illness in Egypt at El-Demerdash tertiary hospitals
Authors: H.A.Agwa Sara , Elghazaly Hesham , El-Nakeep Sarah , Moustafa Ahmad , El-Sayed Manal H , Hafez Hala , abdo Samia , Matboli Marwa , Saad Maha , M.Elsayed Shaimaa , Elsamie Aya M.Abd , M.Dar Reham , elarab Hoda ezz , Ebeid Fatma S. E. , Makkeyah Sara , Meteini Mahmoud S El 
Published: September 2020

Abstract
Purpose: COVID-19 is the most recent pandemic causing morbidity and mortality. Although a part of the pathogens causing SARI, it is unique in causing pulmonary thrombosis and lung fibrosis. Thus different management is needed. We aimed o explore the start of SARS-CoV-2 in preserved SARI samples to know the exact time of its emergence in our hospital, to conduct whole-genome sequencing in positive SARS-CoV-2 samples to define its strain. To assess the clinical characteristics of the severe respiratory infection admitted to El-Demerdash hospitals in that same period from our own file reports.Methods: We conducted a retrospective cohort study among SARI patients who were admitted to Ain Shams university hospitals. preserved nasopharyngeal& oropharyngeal swabs from 333 SARI patients were used in SARS-CoV-2 detection by RT-Real time PCR. Moreover, whole-genome sequencing of SARS-CoV-2 positive samples was performed. Clinical characteristics of the SARI patients were analyzed in the same period to show the relation to morbidity and mortality.Results: The first case of SARS-CoV-2 was detected in a 6months aged female patient on mid-April 2020, B.1.1.7, clade GR. Co-infection (with both bacterial and viral) was most prevalent in pediatrics than adults, but mortality was higher in adults than pediatrics (18.1% versus 7.1%). ICU admission was higher in adults than in the pediatric group (65% versus 12.8%). Co-morbidities were associated with higher mortality and more severe infection. The most common bacterial infection in both adults and pediatrics was Klebsiella pneumoniae, followed by Staphylococcus aureus and Streptococcus pneumoniae. Conclusion: COVID-19 didn’t start till mid-April in the Egyptian hospitals as remarked by this tertiary hospital’s data. Co-infection is the most prevalent in children and further research is needed in this area.
Evaluation of Concentration, RQ, ECR of BTX, MDA Level and DNA Degeneration in Workers Exposed to BTX
Authors: Tualeka Abdul Rohim , Jalaludin Juliana , Fandi Noor Fatihah Mohamad , Russeng Syamsiar S , Ahsan Ahsan , Susilowati Indri H , Rahmawati Pudji , Firdaus Muhamad 
Published: September 2020

Abstract
Introduction. The use of BTX as a solvent in the production process potentially causes negative impact on workers, especially on DNA degeneration and MDA levels in workers of industrial sector.Aims. This study aims to determine the relationship between concentration, RQ and ECR of BTX, MDA levelsand DNA degeneration in industrial workers exposed to BTX in Surabaya.Method. This research was conducted in industries in Surabaya that use BTX as a solvent in its production process. It was conducted in Ketintang, Jemursari, Kalijudan, AUP and Romokalisari. The research design used was cross sectional with 81 samples. The variables studied in this study were the concentration, RQ, ECR of BTX, MDA and DNA degeneration. The data were analyzed using the Pearson correlation test.Result. There was a relationship between the concentration of benzene, toluene and MDA level in workers exposed to BTX with a P value <0.05. There was a relationship between the concentration of toluene, ECR of toluene and DNA degeneration in workers exposed to BTX in the industry in Surabaya with a P value <0.05.Conclusion. There was a relationship between the concentrations of benzene, toluene and MDA levelsin workers exposed to BTX. There was a relationship between toluene concentrations, ECR of toluene and DNA degeneration in workers exposed to BTX.
TGF-β-Induced α-SMA Expression is Mediated by C/EBPβ Acetylation in Human Alveolar Epithelial Cells In Vitro
Authors: Ding Hui , Chen Jinjun , Qin Jingping , Chen Ruhua , Yi Zili 
Published: September 2020

Abstract
This study sought to determine whether binding of acetylated C/EBPβ to α-SMA promoter could affect its activity and was essential for EMT and extracellular matrix deposition in IPF using in vitro model. The expression of EMT and C/EBPβ in A549 cells with TGF-β as pulmonary fibrotic model were detected by western blotting and qPCR. Collagen-I expression using ELISA was performed. The luciferase activity was used to examine the activity of C/EBPβ. Knockdown of C/EBPβ was performed by siRNA. We also investigated the effect of deacetylation of C/EBPβ on EMT using SIRT1. The binding ability of C/EBPβ with α-SMA promoter was affirmed via ChIP and EMSA. The relationship of α-SMA and acetylated C/EBPβ was investigated by Co-IP. SiRNA-mediated knockdown of C/EBPβ in A549 cells attenuated TGF-β1-induced myofibroblast differentiation and ECM deposition. The extent of association between acetylated C/EBPβ and α-SMA promoter was dynamically monitored. It was confirmed that deacetylation of C/EBPβ in A549 cells successfully ameliorated TGF-β1-induced EMT, as shown by reduction in α-SMA expression and excessive collagen-I accumulation. The EMT and fibrotic effect of TGF-β1 dependent on acetylated C/EBPβ-mediated regulation of α-SMA gene activity. C/EBPβ acetylation may play a central role in pulmonary fibrosis.
C3 Glomerulonephritis Along With Light Chain Tubular Reabsorption in Multiple Myeloma: A Case Report
Authors: Xu Junhui , Yu Xiaojuan , Wang Suxia , Yan Miao , Wang Mangju , Ou Jinping , Wang Lihong , Liu Huihui , Cen Xinan 
Published: September 2020

Abstract
Background: Multiple myeloma has different kinds of renal injuries. C3 glomerulonephritis (C3GN) is characterized by abnormal deposition of complement C3 in the glomeruli due to abnormal activation of the alternative pathway of complement system. The association between C3GN and multiple myeloma had been well established. However, the mild renal injury of C3GN in the multiple myeloma patients with high level of light chain had not been reported.Case presentation: A 55 years old Chinese man presented with proteinuria. Combined with immunofixation electrophoresis, bone marrow biopsy and renal biopsy, this patient was diagnosed with IgA type multiple myeloma accompanied by C3GN and light chain tubular reabsorption. Although he had a higher level of lamda serum free light chain, the renal injury of this patient was mild. After treated with 4 courses of BD, one course of PAD and autologous stem cell transplantation, he achieved a very good partial hematologic response with stable renal function. Conclusions: In multiple myeloma, the condition for light chain to result in renal impairment of C3GN is that the light chain reaches a certain level and lasts for a long time. Early diagnosis and early intensive treatment are extremely important for the prognosis of such patients.
Cross-cultural Adaptation and Psychometric Properties of the Mexican Version of the Early Childhood Oral Health Impact Scale (ECOHIS).
Authors: Montoya Alba Lilia Brambila , Knorst Jessica Klöckner , Uribe Isaac Murisi Pedroza , González Rubén Alberto Bayardo , Ardenghi Thiago , Sánchez Carmen Celina Alonso 
Published: September 2020

Abstract
Background: Mexican population still a lack of instruments to assess OHRQoL in preschoolers. To perform the cross-cultural adaptation and evaluate the psychometric properties of the Mexican version of the ECOHIS.Methods: This cross-sectional study was conducted with preschool children from southern Mexico. The investigation was divided into a transcultural adaptation phase and the validation phase. The M-ECOHIS questionnaire was answered by guardians and clinical data were also evaluated. Reliability was evaluated through tests of internal consistency and stability. Convergent and construct validity were also evaluated. The discriminant validity of the M-ECOHIS was determined according to questionnaire scores with oral health measures.Results: A total of 303 preschool children participated in this study. Regarding internal consistency, Cronbach’s alpha was higher 0.78 for the child section, family section, and general M-ECOHIS. The general ICC for the test-retest reliability was 0.95. The correlation between the scores obtained on the child and family impact sections was significant and all items of the M-ECOHIS confirmed the latent variables. M-ECOHIS scores were associated with the presence of untreated dental caries.Conclusion: Our findings suggest that M-ECOHIS is a valid and reliable instrument to assess the impact of oral health on OHRQoL in Mexican preschool children.