bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychosocial distress, clinical variables and self-management activities associated with type 2 diabetes: a study in Ghana
Authors: Amankwah-Poku Margaret , Amoah Albert G. B. , Sefa-Dedeh Araba , Akpalu Josephine 
Published: July 2020

Abstract
Aim
Psychosocial distress can act as a barrier to diabetes self-care management and thus compromise diabetes control. Yet in Ghana, healthcare centres mainly focus on the medical aspect of diabetes to the neglect of psychosocial care. This study determined the relationship amongst psychosocial distress, clinical variables, and self-management activities associated with type 2 diabetes management.

Method
Questionnaires were administered to 162 patients from four hospitals in Accra, Ghana, to assess psychosocial distress (e.g. diabetes distress), clinical variables (e.g. glycaemic control), and self- management activities (e.g. medication intake) related to diabetes. In assessing diabetes distress, the use of the PAID allowed evaluation of broader range of emotional concerns (diabetes-related emotional distress), while the DDS allowed evaluation of factors more closely related to diabetes self-management (diabetes distress).

Results
Diabetes-related emotional distress, diabetes distress and depressive symptoms were reciprocally positively correlated, while non-supportive family behaviour correlated negatively with these psychological variables. Diabetes-related emotional distress correlated positively with systolic and diastolic blood pressure, and correlated negatively with exercise regimen. On the other hand, diabetes distress correlated negatively with dietary and exercise regimen and correlated positively with glycaemic levels, while depressive symptoms correlated positively with glycaemic levels, diabetes complication and systolic blood pressure. Contrary to the literature, non-supportive family behaviour correlated positively with diet, exercise and medication regimen.

Conclusion
The positive association of psychological variables with glycaemic levels and blood pressure levels, and the positive association of non-supportive family behaviour with self-management activities suggests the need for psychosocial care to be incorporate in the management of type 2 diabetes in Ghana. Patients can be screened for diabetes-related distress and symptoms of depression and provided psychosocial care where necessary.
Understanding fully online teaching in vocational education
Authors: Cox Deniese , Prestridge Sarah 
Published: July 2020

AbstractLiterature has previously reported that student-centred practices are the mark of good pedagogy in online education. In contrast, the competency-based nature of vocational education in Australia has been understood to encourage teacher-centred pedagogy. The likely tensions between these two teaching contexts are not yet understood, and little is yet known about the pedagogy of fully online vocational education teachers. To begin understanding pedagogy in this context, a wide-ranging digital survey was implemented. Findings revealed that online vocation education teachers conceived good online pedagogy as student-centred, yet student-student learning opportunities were rated lower than teacher-student practices. Notably, enacted practice was consistently more teacher-centred than teachers’ ideal, and factors within the teaching context were perceived by teachers as a limitation. They reported their workload to be dominated by marking and administration ahead of student-centred practices such as building rapport. This work is of interest to researchers and institutions navigating the continued expansion of online education and the ongoing demand for effective student-centred practice.
Evaluating the effectiveness of a preservice teacher technology training module incorporating SQD strategies
Authors: Hsu Yu-Yin , Lin Chin-Hsi 
Published: July 2020

AbstractPreparation to use information and communication technology (ICT) is an important component of preservice language teachers’ training, and various existing teacher training models propose a range of strategies for increasing their technology knowledge and technology adoption rates. However, the relative effectiveness of these strategies remains unclear. Based on Tondeur et al.’s (2012) Synthesis of Qualitative Data model, which delineates the six main teacher preparation strategies (i.e., role modeling, reflection, instructional design, collaboration, authentic experience, and continuous feedback), the present study designed a 4 week training module for preservice language teachers and examined how these training strategies affected 63 participants’ perceived technology knowledge and attitudes toward technology adoption. Among the six training strategies, reflection and instructional design had the highest positive impacts on these preservice teachers’ self-reported knowledge about and attitudes towards using ICT. As well as revealing the relative impacts of each training strategy, the results indicate that our designed training module has considerable potential for application to teacher training in other subjects.
Phenotypic characterization of the Egyptian isolates “extensively drug-resistant Pseudomonas aeruginosa” and detection of their metallo-β-lactamases encoding genes
Authors: Basha Amr Mohamad , El-Sherbiny Gamal M. , Mabrouk Mona I. 
Published: July 2020

Abstract
Background
Carbapenem antibiotics consider the primary treatment choice for serious Pseudomonas aeruginosa infection. Hence, the evolution of carbapenem resistance mediated by acquiring genes encoding class b enzymes is of global concern. The purpose of this article research is to explore the prevalence, drug resistance profiles, and metallo-β-lactamases (MβLs) production in extensively drug-resistant carbapenem-resistant P. aeruginosa (XDR-CRPA).

Methods
P. aeruginosa isolates were collected and identified according to conventional methods. Antibiotic resistance patterns were determined by single disk diffusion. Minimum inhibitory concentrations (MICs) of (imipenem, meropenem, ceftazidime, piperacillin/tazobactam, levofloxacin, and gentamicin) were determined for CRPA. A subset of the isolates collection consisting of the XDR-CRPA with the highest MICs to imipenem and meropenem were selected for the phenotypic screening of carbapenemases and MβLs production capability using the modified carbapenem inactivation (mCIM) and imipenem-EDTA combined disk (MβL-CD) methods, respectively. Then, molecular analysis, including identification by the specific primer of 16S rRNA and detection of MβL genes using polymerase chain reaction (PCR) was performed to the XDR selected isolates.

Results
Among 100 P. aeruginosa isolated throughout this period, 59% exhibited reduced susceptibility rates to carbapenems. A total of 20.3% and 57% of CRPA isolates were MDR and XDR, respectively. MIC values of the CRPA revealed that these isolates exhibited high MIC50 and MIC90 to the six selected antibiotics. The findings of the (mCIM) assay displayed identical concordance results with the MβL-CD. Molecular investigation technique assured that 10 (90.9%) and 2 (18.1%) of the 11 XDR selected isolates are positive for blaNDM-1 and blaVIM-1 genes, respectively. Polymyxin B and colistin followed by aztreonam were the most effective antibiotics used for curing infections caused by XDR Pseudomonas aeruginosa.

Conclusion
The prevalence of high XDR-CRPA in our study is a critical problem. Our present study found that the blaNDM-1 was present at a significant frequency among the selected XDR isolates, highlighting the need for establishing strict antimicrobial policies to avoid the prompt spread of these isolates.
A case of spastic paraplegia type 11 with a variation in the SPG11 gene
Authors: Elmas Muhsin , Gogus Basak , Değirmenci Banu , Solak Mustafa , Gleeson J. G. 
Published: July 2020

Abstract
Background
Spastic paraplegia 11 (SPG11) is defined as progressive spasticity and weakness of the lower limbs and also associated with mild intellectual disability with learning difficulties in childhood and/or progressive cognitive retardation, peripheral neuropathy, pseudobulbar symptoms, and increased reflexes in the upper limbs. We describe the clinical, laboratory, and radiological presentation of SPG11 through a report of a case and compare with previously reported SPG11 cases in the literature.

Case presentation
This case presents a homozygous variant in the SPG11 gene (NM_025137.4): c.1699C>T;p.(Gln567*).

Conclusion
The diagnosis was made based on molecular findings, thinning of corpus callosum (TCC) and in most cases, periventricular white matter abnormalities are detected in brain MRI. Therefore, the clinical and radiological findings are supporting the diagnosis. However, it should not be forgotten that TCC is not peculiar to SPG11.
Preparation, characterization and evaluation of aspirin: benzoic acid cocrystals with enhanced pharmaceutical properties
Authors: Dutt Braham , Choudhary Manjusha , Budhwar Vikas 
Published: July 2020

Abstract
Background
The cocrystallization process in pharmaceuticals has gained widespread attention as a recent method of modifying physicochemical properties without altering the pharmacological characteristics of drugs. Cocrystallization provides a couple of benefits like it can be employed for a large number of APIs (acidic, basic, ionizable, or non-ionizable), and secondly, the availability of a large number of potential coformers increases the possibility of the cocrystals (CCs) that can be synthesized for an API. The main objective of this study was to investigate the effects of cocrystallization on drugs having poor aqueous solubility.

Results
Aspirin (AN) and benzoic acid (BZ) were cocrystallized by using the solvent evaporation technique. CSD (Cambridge Structure Database) software and ∆pKa value method were used for the selection of the drug and coformer and for prediction of CC formation. The analysis of CCs was performed using DSC (differential scanning calorimetry), FT-IR (Fourier transformation infra-red spectroscopy) and XRD (X-ray diffraction) techniques. In vivo anti-inflammatory studies were conducted on 24 Wistar rats divided into four groups.

Conclusions
Here, in this study, in vitro dissolution studies revealed an improved solubility profile of CCs compared to pure drug and marketed formulation viz. 87%, 31% and 60% respectively. The in vivo anti-inflammatory studies exhibited improved anti-inflammatory activity compared to pure drug. So, on the basis of outcomes of this study, we concluded that cocrystallization process have a direct impact on the improvement of physicochemical characteristics of APIs having issues like solubility or stability without any modification and alteration of their pharmacological actions.
Temperature control of shoot growth and floral initiation in apple (
Malus
×
domestica
Borkh.)
Authors: Heide Ola M. , Rivero Rodmar , Sønsteby Anita 
Published: July 2020

Abstract

Background

The environmental control of flowering in apple (
Malus
×
domestica
Borkh.) has remained an enigma for generations.

Methods
We studied growth and flowering effects of temperature under daylight phytotron conditions in 3-year old ‘Summerred’ and ‘Discovery’ apple trees. Flowering was assessed by dissection of buds on spurs and extension shoots at termination of treatments and flowering performance in the subsequent spring.

Results
Exposure to constant temperatures ranging from 12 to 27 °C for 12 weeks yielded a hyperbolic response curve with optimum at 18–21 °C and little or no flowering at 12 and 27 °C. A drop from high to low temperature after 6 weeks caused growth cessation and initiation of flowering, whereas the reverse shift had the opposite effect. Shoot growth and leaf accumulation increased with increasing temperature, but under flower-inducing conditions, both levelled off and ceased towards the end of the treatment period.

Conclusions
The results are discussed in relation to the extensive physiological and genetic literature on the subject. We interpret the results as two separate effects of temperature on flowering in apple. At 12 °C flowering seems to be limited by low temperature depression of growth and leaf production, while at 27 °C, flowering is blocked by inhibition of the floral initiation itself. Intermediate temperatures of 18–21 °C, on the other hand, seem to satisfy the requirements for both processes. These opposite effects of temperature have apparently confounded the understanding of the environmental control of flowering in apple.
Prevention and Management of Chemotherapy-Induced Peripheral Neuropathy in Survivors of Adult Cancers: ASCO Guideline Update
Authors: Loprinzi Charles L. , Lacchetti Christina , Bleeker Jonathan , Cavaletti Guido , Chauhan Cynthia , Hertz Daniel L. , Kelley Mark R. , Lavino Antoinette , Lustberg Maryam B. , Paice Judith A. , Schneider Bryan P. , Lavoie Smith Ellen M. , Smith Mary Lou , Smith Thomas J. , Wagner-Johnston Nina , Hershman Dawn L. 
Published: July 2020

PURPOSE To update the ASCO guideline on the recommended prevention and treatment approaches in the management of chemotherapy-induced peripheral neuropathy (CIPN) in adult cancer survivors. METHODS An Expert Panel conducted targeted systematic literature reviews to identify new studies. RESULTS The search strategy identified 257 new references, which led to a full-text review of 87 manuscripts. A total of 3 systematic reviews, 2 with meta-analyses, and 28 primary trials for prevention of CIPN in addition to 14 primary trials related to treatment of established CIPN, are included in this update. RECOMMENDATIONS The identified data reconfirmed that no agents are recommended for the prevention of CIPN. The use of acetyl-l-carnitine for the prevention of CIPN in patients with cancer should be discouraged. Furthermore, clinicians should assess the appropriateness of dose delaying, dose reduction, substitutions, or stopping chemotherapy in patients who develop intolerable neuropathy and/or functional impairment. Duloxetine is the only agent that has appropriate evidence to support its use for patients with established painful CIPN. Nonetheless, the amount of benefit from duloxetine is limited. Additional information is available at www.asco.org/survivorship-guidelines .
Critical Care Medicine Practice: A Pilot Survey of US Anesthesia Critical Care Medicine–Trained Physicians
Authors: Siddiqui Shahla , Bartels Karsten , Schaefer Maximilian S. , Novack Lena , Sreedharan Roshni , Ben-Jacob Talia K. , Khanna Ashish K. , Nunnally Mark E. , Souter Michael , Simmons Shawn T. , Williams George 
Published: July 2020

BACKGROUND:
This survey assessed satisfaction with the practice environment among physicians who have completed fellowship training in critical care medicine (CCM) as recognized by the American Board of Anesthesiology (and are members of the American Society of Anesthesiology) and evaluated the perceived effectiveness of training programs in preparing fellows for critical care practice.

METHODS:
A cross-sectional online survey composed of 39 multiple choice and open-ended questions was administered between August and December 2018 to all members of the American Society of Anesthesiologists (ASA) who self-identified as being CCM trained. The survey instrument was developed and revised in an iterative fashion by ASA committee on CCM and the Society for Education in Anesthesia (SEA). Survey results were analyzed using a mixed-method approach.

RESULTS:
Three hundred fifty-three of the 1400 anesthesiologists who self-identified to the ASA as having CCM training (25.2%) completed the survey. Most were men (72.3%), board certified in CCM (98.7%), and had practiced a median of 5 years. Half of the respondents rated their training as “excellent.” A total of 70.6% described currently working in academic centers with 53.6% providing care in open surgical intensive care units (ICUs). Most anesthesiologist intensivists (75%) spend at least 25% of their clinical time providing ICU care (versus clinical anesthesia). A total of 89% of the respondents were involved in educational activities, 60% reported being in administrative leadership roles, and 37% engaged in scholarly activity. Areas of dissatisfaction included fatigue, lack of collegiality or respect, lack of research training, decreased job satisfaction, and burnout. Analysis suggested moderate levels of job satisfaction (49%), work-life balance (52%), and high levels of burnout (74%). A significant contributor to burnout was with a perception of lack of respect (P = .005) in the work environment. Burnout was not significantly associated with gender or duration of practice. Qualitative analysis of the open-ended responses also identified these 3 variables as major themes.

CONCLUSIONS:
This survey of CCM-trained anesthesiologists described a high rate of board certification, practice in academic settings, and participation in resident education. Areas of dissatisfaction with an anesthesia/critical care practice included burnout, work/life balance, and lack of respect. These results may increase recruitment of anesthesiologists into critical care and inform strategies to improve satisfaction with anesthesia critical care practice, fellowship training.
Acute Postoperative Pain Is Associated With Myocardial Injury After Noncardiac Surgery
Authors: Turan Alparslan , Leung Steve , Bajracharya Gausan Ratna , Babazade Rovnat , Barnes Theresa , Schacham Yehoshua Nadav , Mao Guangmei , Zimmerman Nicole , Ruetzler Kurt , Maheshwari Kamal , Esa Wael Ali Sakr , Sessler Daniel I. 
Published: July 2020

BACKGROUND:
Uncontrolled pain after noncardiac surgery activates the sympathetic nervous system, which causes tachycardia, hypertension, and increased cardiac contractility—all of which may increase myocardial oxygen demand. We therefore determined whether time-weighted average pain scores over the initial 72 postoperative hours are associated with myocardial injury after noncardiac surgery (MINS).

METHODS:
We conducted a retrospective cohort analysis of adults with routine postoperative troponin monitoring after noncardiac surgery under general, regional, or combined anesthesia at tertiary level centers in Cleveland from January 2012 to December 2015. Time-weighted average pain scores were calculated from all the available pain scores, typically at 4-hour intervals, until a troponin elevation was detected. MINS was defined as peak troponin T concentrations exceeding 0.03 ng/mL within 72 hours after surgery. We used a generalized linear mixed model to assess the association between pain and MINS with 3 hospitals as clusters, adjusting for potential confounders.

RESULTS:
Among 2892 eligible patients, 4.5% had myocardial injury within 72 hours after surgery. Higher time-weighted average pain scores were associated with increased hazard of myocardial injury. The estimated hazard ratio for a 1-unit increase in pain score was 1.12 (95% confidence interval [CI], 1.02–1.22; P = .013), adjusting for confounding variables.

CONCLUSIONS:
Among patients undergoing noncardiac surgery, time-weighted average pain scores within 72 hours after surgery were significantly associated with myocardial injury.
Removal of remodeling/reprogramming factors from oocytes and the impact on the full-term development of cloned embryos
Authors: Konno Shunsuke , Wakayama Sayaka , Ito Daiyu , Kazama Kousuke , Hirose Naoki , Ooga Masatoshi , Wakayama Teruhiko 
Published: July 2020

ABSTRACT
The reason for the poor development of cloned embryos is not yet clear. Several reports have suggested that some nuclear remodeling/reprogramming factors (RRFs) are removed from oocytes at the time of enucleation, which might cause the low success rate of animal cloning. However, there is currently no method to manipulate the amount of RRFs in oocytes. Here, we describe techniques we have developed to gradually reduce RRFs in mouse oocytes by injecting somatic cell nuclei into oocytes. These injected nuclei were remodeled and reprogrammed using RRFs, and then RRFs were removed by subsequent deletion of somatic nuclei from oocytes. The size of the metaphase II spindle reduced immediately, but did recover when transferred into fresh oocytes. Though affected, the full-term developmental potential of these RRF-reduced oocytes with MII-spindle shrinkage was not lost after fertilization. When somatic cell nuclear transfer was performed, the successful generation of cloned mice was somewhat improved and abnormalities were reduced when oocytes with slightly reduced RRF levels were used. These results suggest that a change in RRFs in oocytes, as achieved by the method described in this paper or by enucleation, is important but not the main reason for the incomplete reprogramming of somatic cell nuclei.
Identification and requirements of enhancers that direct gene expression during zebrafish fin regeneration
Authors: Thompson John D. , Ou Jianhong , Lee Nutishia , Shin Kwangdeok , Cigliola Valentina , Song Lingyun , Crawford Gregory E. , Kang Junsu , Poss Kenneth D. 
Published: July 2020

ABSTRACT
To identify candidate tissue regeneration enhancer elements (TREEs) important for zebrafish fin regeneration, we performed ATAC-seq from bulk tissue or purified fibroblasts of uninjured and regenerating caudal fins. We identified tens of thousands of DNA regions from each sample type with dynamic accessibility during regeneration, and assigned these regions to proximal genes with corresponding expression changes by RNA-seq. To determine whether these profiles reveal bona fide TREEs, we tested the sufficiency and requirements of several sequences in stable transgenic lines and mutant lines with homozygous deletions. These experiments validated new non-coding regulatory sequences near induced and/or essential genes during fin regeneration, including fgf20a, mdka and cx43, identifying distinct domains of directed expression for each confirmed TREE. Whereas deletion of the previously identified LEN enhancer abolished detectable induction of the nearby leptin b gene during regeneration, deletions of enhancers linked to fgf20a, mdka and cx43 had no effect or partially reduced gene expression. Our study generates a new resource for dissecting the regulatory mechanisms of appendage generation and reveals a range of requirements for individual TREEs in control of regeneration programs.
The C-terminal helix of BubR1 is essential for CENP-E-dependent chromosome alignment
Authors: Legal Thibault , Hayward Daniel , Gluszek-Kustusz Agata , Blackburn Elizabeth A. , Spanos Christos , Rappsilber Juri , Gruneberg Ulrike , Welburn Julie P. I. 
Published: July 2020

ABSTRACT
During cell division, misaligned chromosomes are captured and aligned by motors before their segregation. The CENP-E motor is recruited to polar unattached kinetochores to facilitate chromosome alignment. The spindle checkpoint protein BubR1 (also known as BUB1B) has been reported as a CENP-E interacting partner, but the extent to which BubR1 contributes to CENP-E localization at kinetochores has remained controversial. Here we define the molecular determinants that specify the interaction between BubR1 and CENP-E. The basic C-terminal helix of BubR1 is necessary but not sufficient for CENP-E interaction, and a minimal key acidic patch on the kinetochore-targeting domain of CENP-E is also essential. We then demonstrate that BubR1 is required for the recruitment of CENP-E to kinetochores to facilitate chromosome alignment. This BubR1–CENP-E axis is critical for alignment of chromosomes that have failed to congress through other pathways and recapitulates the major known function of CENP-E. Overall, our studies define the molecular basis and the function for CENP-E recruitment to BubR1 at kinetochores during mammalian mitosis.
This article has an associated First Person interview with the first author of the paper.