bluuebunny/crossref_metadata_2025_split huggingface dataset:
EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
Authors: Gimeno-Valiente F. , Riffo-Campos Á. L. , Ayala G. , Tarazona N. , Gambardella V. , Rodríguez F. M. , Huerta M. , Martínez-Ciarpaglini C. , Montón-Bueno J. , Roselló S. , Roda D. , Cervantes A. , Franco L. , López-Rodas G. , Castillo J. 
Published: February 2020

AbstractThe finding of novel molecular markers for prediction or prognosis of invasiveness in colorectal cancer (CRC) constitutes an appealing challenge. Here we show the up-regulation of EPDR1 in a prospective cohort of 101 CRC patients, in a cDNA array of 43 patients and in in silico analyses. EPDR1 encodes a protein related to ependymins, a family of glycoproteins involved in intercellular contacts. A thorough statistical model allowed us to conclude that the gene is significantly up-regulated in tumour tissues when compared with normal mucosa. These results agree with those obtained by the analysis of three publicly available databases. EPDR1 up-regulation correlates with the TNM staging parameters, especially T and M. Studies with CRC cell lines revealed that the methylation of a CpG island controls EPDR1 expression. siRNA knocking-down and overexpression of the gene following transient plasmid transfection, showed that EPDR1 favours cell proliferation, migration, invasiveness and adhesion to type I collagen fibres, suggesting a role in epithelial to mesenchymal transition. Both statistical and functional analysis correlated EPDR1 overexpression with invasiveness and dissemination of tumour cells, supporting the inclusion of EPDR1 in panels of genes used to improve molecular subtyping of CRC. Eventually, EPDR1 may be an actionable target.
The antioxidative enzyme SOD2 is important for physiological persistence of corpora lutea in lynxes
Authors: Braun B. C. , Halaski N. , Painer J. , Krause E. , Jewgenow K. 
Published: February 2020

AbstractCorpora lutea (CL) are transient endocrine glands supporting pregnancy by progesterone production. They develop at the site of ovulation from the remaining follicle, are highly metabolically active and undergo distinct, transformative processes during their lifetime. In contrast to other species, CL of lynxes do not regress at the end of cycle, but remain functionally active (persist) for years. Reactive oxygen species (ROS) and anti-oxidative enzymes are described to be important for the functionality of CL. We examined ten anti-oxidative enzymes in fresh and persistent CL of lynxes as well as in domestic cat CL of different luteal stages. The gene expression profiles, especially those of SOD1 and SOD2, showed some remarkable differences between CL stages during non-pregnant and pregnant cycles of domestic cats and between fresh and persistent CL of lynxes. Lynx gene expression profiles of SODs were confirmed by western blot analysis, immunohistochemistry and activity assays. SOD2 was characterized by a conspicuous high expression and enzyme activity exclusively in persistent CL. We suggest that SOD2 is required to detoxify potential elevated superoxide anion levels by producing H2O2 in the physiologically persistent CL. This product might also act as a signaling molecule, securing the CL from apoptosis and insuring long-term luteal cell survival.
Adventitious shoot organogenesis from leaf explants of Portulaca pilosa L.
Authors: Chen Shuangyan , Xiong Yuping , Yu Xincheng , Pang Jinhui , Zhang Ting , Wu Kunlin , Ren Hai , Jian Shuguang , Teixeira da Silva Jaime A. , Xiong Youhua , Zeng Songjun , Ma Guohua 
Published: February 2020

AbstractThis study established, for the first time, shoot proliferation and plant regeneration protocols via shoot organogenesis from leaf explants of a medical and ornamental plant, Portulaca pilosa L. The optimal proliferation of axillary shoots was 6.2-fold within 30 days on Murashige and Skoog (MS) medium supplemented with 3.0 µM 6-benzyladenine (BA). Shoots could be induced directly from leaf explants, forming an average of 3.8 adventitious shoots per explant, on optimal MS medium supplemented with 1.0 µM thidiazuron (TDZ) and 0.1 µM α-naphthaleneacetic acid (NAA). A higher concentration of TDZ (3.0 µM), alone or in combination with 0.1 µM NAA, induced somatic embryo-like shoot buds and then developed into real shoots. Rooting was easier since roots were induced on all rooting media within one month. Half-strength MS medium free of plant growth regulators was best for rooting. Rooted plantlets were transferred to a sand: perlite (1:1, v/v) substrate, resulting in highest survival (90%). Plantlets showed more robust growth, however, on substrates of yellow mud: perlite (1:1, v/v) or peat soil: vermiculite: perlite (1:1:1, v/v).
Temperature potentially induced distinctive flavor of mud crab Scylla paramamosain mediated by gut microbiota
Authors: Tang Lei , Wang Huan , Wang Chunlin , Mu Changkao , Wei Hongling , Yao Hongzhi , Ye Chunyu , Chen Lizhi , Shi Ce 
Published: February 2020

AbstractMany factors affect the flavor of crabs. However, impact of temperature on flavor has not been reported. Here, we examined Scylla paramamosain collected within the main four producing areas in China from north sampling point (NP) and south sampling point (SP), respectively. The contents of flavouring-related substances in hepatopancreas, muscles and gonads were determined by high-performance liquid chromatography (HPLC). Meanwhile, high-throughput sequencing of 16S RNA gene was used to reveal the diversity distribution of gut microbiota at each sample collection point. Comparisons among flavor substances of edible parts, the implied higher temperature in SP may be beneficial to the accumulation of flavor substances in gonads, while lower temperature in NP may be beneficial to the accumulation of flavor substances in muscles and hepatopancreas. The gut microbiota of crabs, was analyzed via 16S rRNA gene sequencing. The results of gut microbiota showed that there were significant differences in the distribution of gut microbiota in NP and SP. The microbiota composition of SP has a high distribution richness and no absolute dominant bacteria, while NP has absolute dominant bacteria and its microbiota richness was lower than SP. The results of redundancy analysis (RDA) showed that there was a significant correlation between temperature and the relative abundance of gut microbiota, and a significant correlation between gut microbiota and the content of flavor substances. This study indicates that temperature may be one of the main factors for the differences of flavor substances between SP and NP, which was most probably mediated by gut microbiota. Further exploration is needed with laboratory experiments in which the environment is more precisely controlled if these views are to be determined.
High-Performance Conducting Polymer Nanotube-based Liquid-Ion Gated Field-Effect Transistor Aptasensor for Dopamine Exocytosis
Authors: Park Seon Joo , Lee Jiyeon , Seo Sung Eun , Kim Kyung Ho , Park Chul Soon , Lee Sang Hun , Ban Hyun Seung , Lee Byoung Dae , Song Hyun Seok , Kim Jinyeong , Lee Chang-Soo , Bae Joonwon , Kwon Oh Seok 
Published: February 2020

AbstractIn this study, ultrasensitive and precise detection of a representative brain hormone, dopamine (DA), was demonstrated using functional conducting polymer nanotubes modified with aptamers. A high-performance aptasensor was composed of interdigitated microelectrodes (IMEs), carboxylated polypyrrole nanotubes (CPNTs) and DA-specific aptamers. The biosensors were constructed by sequential conjugation of CPNTs and aptamer molecules on the IMEs, and the substrate was integrated into a liquid-ion gating system surrounded by pH 7.4 buffer as an electrolyte. To confirm DA exocytosis based on aptasensors, DA sensitivity and selectivity were monitored using liquid-ion gated field-effect transistors (FETs). The minimum detection level (MDL; 100 pM) of the aptasensors was determined, and their MDL was optimized by controlling the diameter of the CPNTs owing to their different capacities for aptamer introduction. The MDL of CPNT aptasensors is sufficient for discriminating between healthy and unhealthy individuals because the total DA concentration in the blood of normal person is generally determined to be ca. 0.5 to 6.2 ng/mL (3.9 to 40.5 nM) by high-performance liquid chromatography (HPLC) (this information was obtained from a guidebook “Evidence-Based Medicine 2018 SCL “ which was published by Seoul Clinical Laboratory). The CPNTs with the smaller diameters (CPNT2: ca. 120 nm) showed 100 times higher sensitivity and selectivity than the wider CPNTs (CPNT1: ca. 200 nm). Moreover, the aptasensors based on CPNTs had excellent DA discrimination in the presence of various neurotransmitters. Based on the excellent sensing properties of these aptasensors, the DA levels of exogeneous DA samples that were prepared from PC12 cells by a DA release assay were successfully measured by DA kits, and the aptasensor sensing properties were compared to those of standard DA reagents. Finally, the real-time response values to the various exogeneous DA release levels were similar to those of a standard DA aptasensor. Therefore, CPNT-based aptasensors provide efficient and rapid DA screening for neuron-mediated genetic diseases such as Parkinson’s disease.
The difference in dissipation of clomazone and metazachlor in soil under field and laboratory conditions and their uptake by plants
Authors: Szpyrka Ewa , Słowik-Borowiec Magdalena , Książek Paulina , Zwolak Aneta , Podbielska Magdalena 
Published: February 2020

AbstractThe study concerned dissipation of metazachlor and clomazone, herbicides widely used in rapeseed (Brassica napus L. subsp. napus) protection, applied to the clay soil under field and laboratory conditions. Furthermore, the uptake of these pesticide from soil by rapeseed plants was investigated under field conditions. An additional aim of this work was to modify the QuEChERS method for the determination of metazachlor and clomazone in the plant material. Analytical procedures for metazachlor and clomazone qualification and quantification in rapeseed plants and soil were developed, using gas chromatography with an micro electron capture detector (GC-μECD) and a mass detector (GC-MS/MS QqQ) as confirmation. Dissipation kinetics of herbicide residues in soil were described as first-order equations. The analytical performance was very satisfactory and confirmed that the methods meet the requirements of the European Commission. In the conducted field experiments it was found that dissipation of clomazone and metazachlor in clay soil follows first-order kinetics (R2 between 0.964 and 0.978), and half-lives were 9.5 days and 10.2 days for clomazone and metazachlor, respectively. Under laboratory conditions, dissipation of clomazone and metazachlor in soil also follows first-order kinetics (R2 between 0.937 and 0.938), and half-lives were 8.8 days and 5.7 days for clomazone and metazachlor, respectively. Residues of both herbicides in rape plants 22 days after application of herbicides were below the maximum residue levels for Brassica plants. Metazachlor and clomazone dissipate very fast in clay soil and their uptake by rape plants is very low.
The Dependence of Implicit Solvent Model Parameters and Electronic Absorption Spectra and Photoinduced Charge Transfer
Authors: Chen Xiangtao , Qiao Wenhua , Miao Wenjing , Zhang Yangdong , Mu Xijiao , Wang Jingang 
Published: February 2020

AbstractIn this work, the relationship between multiple solvent parameters and charge transfer index was analyzed by multi-factor multi-variate partial least squares regression (PLSR). The charge transfer of the molecule is visualized by the analysis of the excited state wave function. Hydrogen bond basicity and surface tension can significantly affect charge transfer by studying the solvation model parameters and charge transfer index. Finally, a method in which a solvent regulates charge transfer strength and migration length is proposed.
Longitudinal DNA methylation differences precede type 1 diabetes
Authors: Johnson Randi K. , Vanderlinden Lauren A. , Dong Fran , Carry Patrick M. , Seifert Jennifer , Waugh Kathleen , Shorrosh Hanan , Fingerlin Tasha , Frohnert Brigitte I. , Yang Ivana V. , Kechris Katerina , Rewers Marian , Norris Jill M. 
Published: February 2020

AbstractDNA methylation may be involved in development of type 1 diabetes (T1D), but previous epigenome-wide association studies were conducted among cases with clinically diagnosed diabetes. Using multiple pre-disease peripheral blood samples on the Illumina 450 K and EPIC platforms, we investigated longitudinal methylation differences between 87 T1D cases and 87 controls from the prospective Diabetes Autoimmunity Study in the Young (DAISY) cohort. Change in methylation with age differed between cases and controls in 10 regions. Average longitudinal methylation differed between cases and controls at two genomic positions and 28 regions. Some methylation differences were detectable and consistent as early as birth, including before and after the onset of preclinical islet autoimmunity. Results map to transcription factors, other protein coding genes, and non-coding regions of the genome with regulatory potential. The identification of methylation differences that predate islet autoimmunity and clinical diagnosis may suggest a role for epigenetics in T1D pathogenesis; however, functional validation is warranted.
Neuron-specific analysis of histone modifications with post-mortem brains
Authors: Koshi-Mano Kagari , Mano Tatsuo , Morishima Maho , Murayama Shigeo , Tamaoka Akira , Tsuji Shoji , Toda Tatsushi , Iwata Atsushi 
Published: February 2020

AbstractHistone modifications govern chromatin structures and regulate gene expression to orchestrate cellular functions in the central nervous system, where neuronal cells are postmitotic and developmentally inactive, the functional and age-dependent changes also accumulate in the epigenetic states. Because the brain is composed of several types of cells, such as the neurons, glial cells, and vascular cells, the analysis of histone modifications using bulk brain tissue might obscure alterations specific to neuronal cells. Furthermore, among the various epigenetic traits, analysis of the genome-wide distribution of DNA methylation in the bulk brain is predominantly a reflection of DNA methylation of the non-neuronal cells, which may be a potential caveat of previous studies on neurodegenerative diseases using bulk brains. In this study, we established a method of neuron-specific ChIP-seq assay, which allows for the analysis of genome-wide distribution of histone modifications specifically in the neuronal cells derived from post-mortem brains. We successfully enriched neuronal information with high reproducibility and high signal-to-noise ratio. Our method will further facilitate the understanding of neurodegeneration.
Tied-array holography with LOFAR
Authors: Salas P. , Brentjens M. A. , Bordenave D. D. , Oonk J. B. R. , Röttgering H. J. A. 
Published: February 2020

Context. A radio interferometer uses time delays to maximize its response to radiation coming from a particular direction. These time delays compensate for differences in the time of arrival of the wavefront at the different elements of the interferometer, and for delays in the instrument’s signal chain. If the radio interferometer is operated as a phased array (tied array), the time delays cannot be accounted for after an observation, so they must be determined in advance.
Aims. Our aim is to characterize the time delays between the stations in the core of the LOw Frequency ARray (LOFAR).
Methods. We used radio holography to determine the time delays for the core stations of LOFAR (innermost 3.5 km). Using the multibeaming capability of LOFAR we map the voltage beam faster than with a raster scan, while simultaneously calibrating the observed beam continuously.
Results. For short radio holographic observations (60 s and 600 s) of 3C196, 3C147, and 3C48 we are able to derive time delays with errors of less than one nanosecond. After applying the derived time delays to the beamformer, the beam shows residuals of less than 20% with respect to the theoretical beam shape.
Conclusions. Tied-array holography could be a way towards semi-real-time beam calibration for the Square Kilometer Array.
Avaliação do tratamento de pacientes submetidos a osteogênese por distração na tíbia
Authors: de Resende Henrique Carvalho , Gomes Diogo de Vasconcelos Sabido , Mota Gabriel Milhomem da Silva 
Published: February 2020

Resumo
Objetivo Avaliar o tratamento das fraturas de tíbia que evoluíram com perda óssea (traumática ou secundária a infecção) e as complicações ocorridas durante o tratamento com fixador externo e no período imediatamente após sua retirada.
Métodos Foram selecionados 40 pacientes tratados entre 2010 e 2017, com a idade média de 33,02 anos, sendo 34 do sexo masculino e 6 do sexo feminino. Todos os pacientes portavam regenerado ósseo da tíbia, foram vítimas de trauma (sobretudo motociclístico), e estavam em seguimento ambulatorial.
Resultados Foram obtidos regenerados ósseos da tíbia proximal de até 17 cm e da tíbia distal de 14 cm. O maior transporte trifocal teve a soma do tamanho dos tecidos dos ossos regenerados, medindo 14,5 cm. Como complicações, 29 (72,5%) pacientes tiveram infecção no trajeto dos pinos e fios. Houve 9 (22,5%) casos de refratura, sendo 6 deles tratadas com novo fixador circular, e 2 infecções no osso regenerado. Os pacientes foram submetidos a uma média de 4,72 procedimentos cirúrgicos (2–12), portaram fixador por 20,75 meses (7–55 m.) e permaneceram internados por 53,7 dias (5–183) devido principalmente a complicações de partes moles, a antibioticoterapia intravenosa ou até mesmo a questões sociais. Dois (5%) pacientes apresentaram gonartrose sintomática e outros 2 artrite sintomática do tornozelo. Três apresentaram discrepância de membros inferiores de 3,0; 3,7; e 5,0 cm.
Conclusão Apesar de não ser um método de tratamento amplamente disponível, o método de Ilizarov é útil para solucionar a maioria das falhas ósseas da tíbia, independente da sua etiologia.
Uso de placa retorcida – Um método de fixação da fíbula distal
Authors: Agarwala Sanjay , Menon Aditya , Bhadiyadra Ravi 
Published: February 2020

Resumo
Objetivo A literatura discute diversos métodos intramedulares ou extramedulares para fixação de fraturas da fíbula distal, mas não há consenso acerca do método ideal de fixação. Analisamos retrospectivamente os resultados do uso de uma placa bloqueada de compressão (LCP) de 3,5 mm retorcida e com contorno como placa de apoio posterior.
Métodos Dos 62 casos de fraturas de tornozelo tratadas em nosso instituto pelo autor sênior entre 1° de janeiro de 2012 e 31 de dezembro de 2015, 41 pacientes atenderam aos critérios de inclusão (tipos B e C de Danis-Weber).
Resultados Todas as 41 fraturas fibulares distais cicatrizaram sem intercorrências, em uma média de 10,4 semanas (8–14 semanas) (Figuras 6 a 9) e sem complicações. A pontuação American Orthopaedic Foot & Ankle Society (AOFAS) média foi de 92,6 (86–100) em um período médio de acompanhamento de 31,5 meses (14–61 meses).
Conclusões Obtivemos excelentes resultados clínicos e radiológicos com uso de LCP retorcida de 3,5 mm como apoio posterior ao combinar as vantagens da placa antideslizante posterior e a LCP lateral.
National Survey on Training in Pediatric Cardiology by the “Junges Forum” of the DGPK
Authors: Horst Jan-Pit , Michel Miriam , Kubicki Rouven , Lang Nora , Zschirnt Martin , Moosmann Julia 
Published: February 2020

Abstract
Background This is the first national survey of residents and fellows in pediatric cardiology in Germany evaluating training, research activity, and the general working environment.
Methods An online questionnaire including 62 questions (SurveyMonkey) was developed by the “Junges Forum” of the German Society of Pediatric Cardiology. Fellows and residents during training and up to 3 years after completing their pediatric cardiology fellowship were invited to participate.
Results A total of 102 pediatric cardiology fellows and residents completed the questionnaire. Many participants complained about their training as being unstructured (47%) and non-transparent (37%). The numbers of technical and catheter interventions required by the national medical board in Germany cannot be achieved, especially regarding invasive procedures. Sixty per cent work more than contractually agreed, usually in Germany it is 40 hours daytime work plus on calls, while 90% of all participants prefer less than 50 weekly working hours; 50% of the participants are engaged in research that is usually done during their spare time. More than 90% are satisfied with their professional relationships with colleagues and coworkers. Seventy-eight per cent describe their career perspectives as promising, and 84% would start a fellowship in pediatric cardiology again.
Conclusion The majority of pediatric cardiology fellows and residents are satisfied with their working environment and with their choice of a career in pediatric cardiology. Besides the heavy work load, we identified the urgent desire for better structured transparent clinical training concept including the teaching of manual skills, i.e., invasive procedures and catheterization.
Dreams and realities of school tracking and vocational education
Authors: Ozer Mahmut , Perc Matjaž 
Published: February 2020

AbstractSchool tracking has been introduced as a means to provide skills the labor market demands, and as such has been in place for several decades in most Organisation for Economic Co-operation and Development (OECD) countries. The time is thus ripe for a critical review of the effects this has had on the equalities in education and opportunities later in life, and on the quality of vocational education in general. A synthesis of the existing literature reveals gaping holes between the dreams of superior vocational education and training that educational tracking ought to deliver, and the realities of lost opportunities and facilitated inequalities, especially in students with poor socioeconomic background, weak social capital, and sparse social networks. This is all the more true the sooner educational tracking comes into effect. While most OECD countries will start tracking students aged 15 or 16, some countries, such as Germany, will start doing this as early as age 10. Our review shows that this can have catastrophic consequences for students that for various reasons perform poorly early on, as they are indeed unable to recover due to the Matthew effect and preferential attachment in social networks, both of which punish false starts in life and reward first movers. To remedy the situation, we propose educational tracking be held off until later in life, and even then be undertaken with flexibility and late bloomers in mind. We also propose to restructure vocational education by decreasing the degree of curriculum differentiation, by allowing broader vocational education curricula, and by decreasing the number of training occupations in order to account for the changing labor market dynamics.
Attractors of nonlinear Hamiltonian partial differential equations
Authors: Komech A. I. , Kopylova E. A. 
Published: February 2020

Abstract
This is a survey of the theory of attractors of nonlinear Hamiltonian partial differential equations since its appearance in 1990. Included are results on global attraction to stationary states, to solitons, and to stationary orbits, together with results on adiabatic effective dynamics of solitons and their asymptotic stability, and also results on numerical simulation. The results obtained are generalized in the formulation of a new general conjecture on attractors of

-invariant nonlinear Hamiltonian partial differential equations. This conjecture suggests a novel dynamical interpretation of basic quantum phenomena: Bohr transitions between quantum stationary states, de Broglie’s wave-particle duality, and Born’s probabilistic interpretation.
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