bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of organoid cultured human breast cancer
Authors: Goldhammer Nadine , Kim Jiyoung , Timmermans-Wielenga Vera , Petersen Ole William 
Published: December 2019

AbstractOrganoid cultures are increasingly used to model human cancers experimentally with a view to tailoring personalized medicine and predicting drug responses. Breast cancer is no exception, but in particular, primary breast cancer poses some inherent difficulties due to the frequent presence of residual non-malignant cells in the biopsies. We originally developed an assay for the distinction between malignant and non-malignant structures in primary breast cancer organoid cultures (Petersen et al., Proc Natl Acad Sci (USA) 89(19):9064–8, 1992). Here, we apply this assay to assess the frequency of normal-like organoids in primary breast carcinoma cultures and the cellular composition as a consequence of passaging. We find that in consecutively collected samples of primary human breast cancers, residual non-malignant tissues were observed histologically in five out of ten biopsies. Based on relevant morphogenesis and correct polarization as recorded by expression in luminal epithelial cells of mucin 1 (Muc1), occludin, and keratin 19 (K19) and expression in basal cells of integrin β4, p63, and K14, non-malignant organoids were present in all primary human breast cancer-derived cultures. Furthermore, passaging in a contemporary culture medium was in favor of the selective expansion of basal-like cells. We conclude that organoid cultures of human breast cancers are most representative of the tissue origin in primary culture.
Acupoint transcutaneous electrical nerve stimulation in hospitalized COPD patients with severe dyspnoea: study protocol for a randomized controlled trial
Authors: Fernández-Jané Carles , Vilaró Jordi 
Published: December 2019

Abstract
Background
Acute exacerbation of chronic obstructive pulmonary disease (AECOPD) is a major cause of hospital admissions and dyspnoea is its main symptom. Some studies have concluded that a new modality of acupuncture, called acupoint transcutaneous electrical nerve stimulation (acuTENS), could reduce dyspnoea in patients with COPD by increasing β-endorphin levels. However, those trials have been conducted mainly on patients in stable condition. This study aims to determine whether the administration of acuTENS can reduce dyspnoea in patients hospitalized for AECOPD.

Methods
A multicentre randomized control trial with blinding of participants and assessors will be conducted. A sample of 60 patients will be randomly assigned to receive 45 min of either real acuTENS or sham acuTENS treatment once a day for five consecutive days. The trial will be conducted at the “Hospital del Mar” in Barcelona (Spain) and the “Hospital Sant Joan de Déu de Manresa” in Manresa (Spain). The Borg scale at baseline and days 1 to 5 will be the primary outcome. Secondary outcomes will be the duration of the hospitalization, quantity of drugs administrated, expiratory peak flow adverse effects and mortality and readmissions at 3 months.

Discussion
AcuTENS is a non-pharmacological, non-invasive and inexpensive intervention. This trial will help to elucidate the potential role of acuTENS in the treatment of AECOPD.

Trial registration
ClinicalTrials.gov identifier: NCT02998957. Recruitment status: Recruiting. First posted: 21 December 2016. Last update posted: 2 October 2018. Trial registration dataset is available in Additional file 1. Protocol version 03. Issue date: 20 March 2018. Author: Carles Fernández.
Efficacy and safety of dexmedetomidine for prevention of withdrawal syndrome in the pediatric intensive care unit: protocol for an adaptive, multicenter, randomized, double-blind, placebo-controlled, non-profit clinical trial
Authors: Mondardini Maria Cristina , Sperotto Francesca , Daverio Marco , Caramelli Fabio , Gregori Dario , Caligiuri Maria Francesca , Vitale Francesca , Cecini Maria Teresa , Piastra Marco , Mancino Aldo , Pettenazzo Andrea , Conti Giorgio , Amigoni Angela 
Published: December 2019

Abstract
Background
Prolonged treatment with analgesic and sedative drugs in the pediatric intensive care unit (PICU) may lead to undesirable effects such as dependence and tolerance. Moreover, during analgosedation weaning, patients may develop clinical signs of withdrawal, known as withdrawal syndrome (WS). Some studies indicate that dexmedetomidine, a selective α2-adrenoceptor agonist, may be useful to prevent WS, but no clear evidence supports these data. The aims of the present study are to evaluate the efficacy of dexmedetomidine in reducing the occurrence of WS during analgosedation weaning, and to clearly assess its safety.

Methods
We will perform an adaptive, multicenter, randomized, double-blind, placebo-controlled trial. Patients aged < 18 years receiving continuous intravenous analgosedation treatment for at least 5 days and presenting with clinical conditions that allow analgosedation weaning will be randomly assigned to treatment A (dexmedetomidine) or treatment B (placebo). The treatment will be started 24 h before the analgosedation weaning at 0.4 μg/kg/h, increased by 0.2 μg/kg/h per hour up to 0.8 μg/kg/h (neonate: 0.2 μg/kg/h, increased by 0.1 μg/kg/h per hour up to 0.4 μg/kg/h) and continued throughout the whole weaning time. The primary endpoint is the efficacy of the treatment, defined by the reduction in the WS rate among patients treated with dexmedetomidine compared with patients treated with placebo. Safety will be assessed by collecting any potentially related adverse event. The sample size assuring a power of 90% is 77 patients for each group (total N = 154 patients). The study was approved by the Ethics Committee of the University-Hospital S.Orsola-Malpighi of Bologna on 22 March 2017.

Discussion
The present trial will allow us to clearly assess the efficacy of dexmedetomidine in reducing the occurrence of WS during weaning from analgosedation drugs. In addition, the study will provide a unique insight into the safety profile of dexmedetomidine.

Trial registration
ClinicalTrials.gov, NCT03645603. Registered on 24 August 2018.
EudraCT, 2015–002114-80. Retrospectively registered on 2 January 2019.
Management of adult-onset Still’s disease with interleukin-1 inhibitors: evidence- and consensus-based statements by a panel of Italian experts
Authors:   AOSD Consensus Group, Colafrancesco Serena , Manara Maria , Bortoluzzi Alessandra , Serban Teodora , Bianchi Gerolamo , Cantarini Luca , Ciccia Francesco , Dagna Lorenzo , Govoni Marcello , Montecucco Carlomaurizio , Priori Roberta , Ravelli Angelo , Sfriso Paolo , Sinigaglia Luigi 
Published: December 2019

AbstractBackgroundAdult-onset Still’s disease (AOSD) is a rare inflammatory condition characterized by fever, rash, and arthritis. Because of its rarity, clinical trials are inherently small and often uncontrolled. Our objective was to develop recommendations for the use of interleukin (IL)-1 inhibitors in the management of patients with AOSD, based on the best evidence and expert opinion.MethodsA panel of 10 experts (9 rheumatologists and 1 pediatrician) was established. The first step was dedicated to a comprehensive literature review and development of statements. Two separate literature searches were performed on the MEDLINE (Pubmed), EMBASE, and BIOSIS databases through April 2018 to identify (1) differences and similarities between AOSD and pediatric Still’s disease (systemic juvenile idiopathic arthritis [SJIA]) and (2) the efficacy and safety of IL-1 inhibitors in AOSD treatment. In the second step, the statements were submitted in a Delphi process to a panel of 67 rheumatologists. Consensus threshold was set at 66%: positive, > 66% of voters selected scores 3 to 5; negative, > 66% of voters selected scores 1 or 2. In the third step, the voting results were analyzed, and the statements were finalized.ResultsEleven statements were developed. Forty-six of 67 rheumatologists (72%) participated in the Delphi process. A positive consensus was reached after the first round of voting and was full (> 95%) on the majority of statements. A large consensus was achieved in considering AOSD and SJIA as the same disease. The use of anti-IL-1 therapies in refractory patients was considered quite safe and effective both as the first and as a subsequent line of biologic treatment, especially in systemic patients. Because of the lack of head-to-head comparisons, a different profile of efficacy among IL-1 inhibitors could not be established. There was a large consensus that failure of the first IL-1 inhibitor does not preclude response to another one. The lack of studies comparing early versus late treatment did not allow to draw conclusions; however, data from SJIA suggest a better response in early treatment.ConclusionsThe Delphi method was used to develop recommendations that we hope will help clinicians in the management of patients with AOSD refractory to conventional therapies.
The comparative efficacy and risk of harms of the intravenous and subcutaneous formulations of trastuzumab in patients with HER2-positive breast cancer: a rapid review
Authors: Van den Nest Miriam , Glechner Anna , Gold Maria , Gartlehner Gerald 
Published: December 2019

AbstractBackgroundTrastuzumab is a monoclonal antibody for patients with HER2 (human epidermal growth factor receptor 2)-positive breast cancer, which is added to regular treatment and reduces mortality. Originally, trastuzumab had to be administered intravenously (IV) over 30 min every 3 weeks for 1 year. Since 2012, a formulation for the subcutaneous (SC) administration of trastuzumab has been available, which has not yet been approved in the USA. Advocates claim that the SC formulation saves time and money, despite higher costs. The purpose of this study is to review existing literature concerning the comparative efficacy and risk of harms of trastuzumab IV and SC concerning patient-relevant health outcomes.MethodsWe conducted searches in the Cochrane Library and MEDLINE for articles published through May 2018 in English or German. In addition, we searchedClinicalTrials.govto identify unpublished studies. We dually reviewed the abstracts and full-text articles based on a priori defined inclusion criteria, rated the risk of bias of included studies, and assessed the strength of the evidence for each outcome of interest. Because data was insufficient for quantitative synthesis, we summarized results narratively.ResultsWe identified three RCTs (randomized controlled trials) meeting our eligibility criteria, which included data on 1003 patients. We found moderate evidence for similar event rates (20.05% vs. 18%, HR (hazard ratio) 0.88, CI 95% = 0.62–1.27), and mortality rates (10% vs. 8%, HR 0.76, CI 95% = 0.44–1.32) after 1.7 years for patients receiving trastuzumab IV and for patients receiving SC. Results remained similar after 3.3 years, though evidence lacked strength due to a high dropout rate. All trials reported more adverse events among the SC group than in the IV group. Evidence for these findings was of moderate strength. Nevertheless, more than 85% of the patients preferred trastuzumab SC over IV. Results concerning serious adverse events appeared to be heterogeneous.ConclusionResults of studies indicate similar efficacy between the two routes of administration. The higher rates of adverse events for SC administration were mainly attributable to injection site–related events. The clinical decision of whether to administer trastuzumab SC or IV requires the consideration of several factors and should be determined individually.
Uniqueness of meromorphic functions with their reduced linear c-shift operators sharing two or more values or sets
Authors: Banerjee Abhijit , Bhattacharyya Saikat 
Published: December 2019

AbstractIn the paper, we introduce a new notion of reduced linear c-shift operator $L _{c}^{r}\,f$Lcrf, and with the aid of this new operator, we study the uniqueness of meromorphic functions $f(z)$f(z) and $L_{c}^{r}\,f$Lcrf sharing two or more values in the extended complex plane. The results obtained in the paper significantly improve a number of existing results. Further, using the notion of weighted sharing of sets, we deal the same problem. We exhibit a handful number of examples to justify certain statements relevant to the content of the paper. We are also able to determine the form of the function that coincides with its reduced linear c-shift operator. At the end of the paper, we pose an open question for future research.
Gendered dynamics of transnational social protection
Authors: Bilecen Başak , Barglowski Karolina , Faist Thomas , Kofman Eleonore 
Published: December 2019

AbstractSocial protection refers to resources and strategies to deal with social risks, such as poverty or obligations and needs of care, which might impede the realization of life chances and well-being. Previous research has shown that migrants are particularly affected by challenges when accessing or providing social protection, because of unfamiliar welfare regulations in the immigration country and their family and friends being located in various locations, calling for an investigation of gendered dynamics in transnational spaces. Inthis special issuewe aim to advance these vital debates by elucidating the social consequences of the articulation and organization of formal and informal social protection across borders for different actors involved through a joint investigation of gender and transnationality as key dimensions of social inequality. More specifically, the papers collected inthis special issueare devoted to investigating the link of formal and informal dimensions of transnational social protection and showing its impact on unequal life chances of mobile people in Europe. This introduction frames the articles collected inthis special issuefrom an inequality perspective, thereby pointing to the ways in which gender and transnationality interact with other dimensions of inequality in the field of social protection, which currently constitutes one of the most vital issues in the field of migration scholarship.
Disease surveillance using online news: an extended study of dengue fever in India
Authors: Zhang Yiding , Ibaraki Motomu , Schwartz Franklin W. 
Published: December 2019

Abstract
Background
The study demonstrates the potential in using newspaper information as a proxy for monitoring dengue fever outbreaks in India. Online newspapers are being considered as sources of information on disease surveillance, early outbreak detection, and epidemiology research. Our objective is to understand the complex dengue epidemiology and discover inter-relationships between dengue fever and local social-environmental factors by mining information from local Indian news articles.

Results
We search and extract articles from the newspaper database, LexisNexis. News articles related to dengue fever in India are analyzed together with local environmental, climate, and population data in both temporally and spatially to study disease epidemiology. We also examine the influence of newsworthiness for constructing a disease surveillance system. In terms of temporal aspects, dengue outbreaks follow consistent patterns every year. However, for many areas, this application is frustrated by the relatively small numbers of news articles.

Conclusions
The study has advanced capabilities in producing approaches that provide for richer interpretations of textual information provided in newspaper articles. Such approaches appear particularly well suited for developing countries with relatively poor medical infrastructures and records.
Excited delirium syndrome from psychostimulant abuse can mimic a violent scene of death
Authors: Jothee Shatishraj , Shafie Mohamed Swarhib , Nor Faridah Mohd 
Published: December 2019

Abstract
Background
Previous reported cases on excited delirium syndrome studied on the common clinical manifestations of the syndrome. The usual forensics implication for the syndrome is that death commonly is associated with restraint procedures by law enforcement agencies; however, not many cases reported highlights the difficulties in attributing a violent scene of death to the syndrome.

Case presentation
We present a case of a partially naked body found in an apartment unit under suspicious circumstances with multiple injuries. The scene of death was violent, and the body was found with blood wiped all over the floor and walls. Investigators believed a violent crime had occurred, and a suspect was reprimanded. However, upon autopsy, it was found that all injuries were superficially inflicted and were unlikely to have been part of an act of commission or caused his death. Internal examination found no remarkable pathology. Toxicology revealed a presence of psychostimulants, that is, methamphetamine, MDMA, and ethyl alcohol. Reconstruction of events by the witness, who was initially suspected of the ‘murder’, revealed that the injuries and his death could likely be explained by an episode of excited delirium.

Conclusion
The case highlights the challenges faced when attributing excited delirium syndrome as a cause of death. The syndrome can present with injuries from aggressive or bizarre behaviour, coupled with the destruction of property, which may confuse investigators on the possible manner of death.
Role of multidetector CT virtual cystoscopy compared to conventional cystoscopy in the diagnosis of urinary bladder neoplasms
Authors: Niazi Gamal , Hetta Waleed M. 
Published: December 2019

Abstract
Background
The study was done to assess the utility of multidetector CT virtual cystoscopy versus conventional cystoscopy in the diagnosis of urinary bladder tumors. Thirty patients were included, 23 patients with recently diagnosed bladder masses, 7 patients with history of previously resected bladder masses. Conventional cystoscopy was done to all patients with biopsy of suspicious lesions. CT examinations were performed with a 16 and a 320-MDCT scanners. Multiplanar reconstructed (MPR) images were obtained. Also virtual cystoscopic images were obtained with the volume rendering technique and the same software used for MPR images.

Results
CTVC detected 9 out of 10 polypoid masses detected by conventional cystoscopy (90% sensitivity), 23 out of 23 sessile masses (100% sensitivity), 4 out of 4 areas of wall thickening (100% sensitivity), and 0 out of 1 case mucosal color change (0% sensitivity).

Conclusion
CTVC is a minimally invasive technique that can be of value for primary diagnosis, surveillance and screening of urinary bladder neoplasms.
Developing of the smart quadcopter with improved flight dynamics and stability
Authors: Malik Waqas , Hussain Sakhawat 
Published: December 2019

AbstractThe cause of the rapid growth and enhancement in the dynamics of unmanned aerial vehicles (UAVs) is due to its vast utilization in every-day application. The major advantage of UAVs is no risk of human life with secure and suitable surveillance. The UAV facilitates in live video streaming and wide aerial coverage for monitoring. In this project, a type of UAV named quadcopter has been developed. The work mainly consists of design of the quadcopter frame, interfacing of the brushless DC motors and bluetooth module with the microcontroller, and adjustment of the roll, pitch, and yaw for keeping the smooth flight dynamics by flight controller board containing MEMS sensors, i.e., gyroscope, accelerometer, magnetometer, and pressure sensors. The repeated simulation and testing has been carried out in MATLAB for the mathematical modeling of the dynamics of the system, i.e., Euler method for solving differential equation for finding system states, computation of the rotation matrix R and functions to convert from an angular velocity vector w to the derivatives of roll, pitch, and yaw. The improvement in the parameters of the flight dynamics and removing the errors to gain stability in the frame are the main core issues which have been achieved by the several iterations. A closed-loop control system, i.e., PID controller, has been simulated and carefully designed for stabilizing the actual angle from the sensors and desired angle from the pilot. The reduced error rate of 0.05 degrees after every 10 s was achieved.
Outcome in Children With Standard-Risk B-Cell Acute Lymphoblastic Leukemia: Results of Children’s Oncology Group Trial AALL0331
Authors: Maloney Kelly W. , Devidas Meenakshi , Wang Cindy , Mattano Leonard A. , Friedmann Alison M. , Buckley Patrick , Borowitz Michael J. , Carroll Andrew J. , Gastier-Foster Julie M. , Heerema Nyla A. , Kadan-Lottick Nina , Loh Mignon L. , Matloub Yousif H. , Marshall David T. , Stork Linda C. , Raetz Elizabeth A. , Wood Brent , Hunger Stephen P. , Carroll William L. , Winick Naomi J. 
Published: December 2019

PURPOSE Children’s Oncology Group (COG) AALL0331 tested whether intensified postinduction therapy that improves survival in children with high-risk B-cell acute lymphoblastic leukemia (ALL) would also improve outcomes for those with standard-risk (SR) ALL. PATIENTS AND METHODS AALL0331 enrolled 5,377 patients between 2005 and 2010. All patients received a 3-drug induction with dexamethasone, vincristine, and pegaspargase (PEG) and were then classified as SR low, SR average, or SR high. Patients with SR-average disease were randomly assigned to receive either standard 4-week consolidation (SC) or 8-week intensified augmented Berlin-Frankfurt-Münster (BFM) consolidation (IC). Those with SR-high disease were nonrandomly assigned to the full COG-augmented BFM regimen, including 2 interim maintenance and delayed intensification phases. RESULTS The 6-year event-free survival (EFS) rate for all patients enrolled in AALL0331 was 88.96% ± 0.46%, and overall survival (OS) was 95.54% ± 0.31%. For patients with SR-average disease, the 6-year continuous complete remission (CCR) and OS rates for SC versus IC were 87.8% ± 1.3% versus 89.1% ± 1.2% ( P = .52) and 95.8% ± 0.8% versus 95.2% ± 0.8% ( P = 1.0), respectively. Those with SR-average disease with end-induction minimal residual disease (MRD) of 0.01% to < 0.1% had an inferior outcome compared with those with lower MRD and no improvement with IC (6-year CCR: SC, 77.5% ± 4.8%; IC, 77.1% ± 4.8%; P = .71). At 6 years, the CCR and OS rates among 635 nonrandomly treated patients with SR-high disease were 85.55% ± 1.49% and 92.97% ± 1.08%, respectively. CONCLUSION The 6-year OS rate for > 5,000 children with SR ALL enrolled in AALL0331 exceeded 95%. The addition of IC to treatment for patients with SR-average disease did not improve CCR or OS, even in patients with higher MRD, in whom it might have been predicted to provide more value. The EFS and OS rates are excellent for this group of patients with SR ALL, with particularly good outcomes for those with SR-high disease.