bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR‐20a‐5p promotes pulmonary artery smooth muscle cell proliferation and migration by targeting ABCA1
Authors: Zhou Yun , Fang Xuan‐liang , Zhang Yun , Feng Yan‐ni , Wang Shan‐shan 
Published: July 2020

AbstractBackgroundThe function of miR‐20a‐5p in pulmonary artery smooth muscle cells (PASMCs) and the underlying mechanism remains largely unknown.MethodsC57BL/6J mice and PASMCs were used for constructing pulmonary artery hypertension (PAH) animal and cell models, respectively. Reverse transcription polymerase chain reaction (RT‐PCR) was employed to detect miR‐20a‐5p and ATP‐binding cassette subfamily A member 1 (ABCA1) messenger RNA expression. CCK‐8, Transwell, and TUNEL experiments were used to determine PASMCs proliferation, migration, and apoptosis. The relationship between miR‐20a‐5p and ABCA1 was detected by luciferase reporter experiment, Western blot analysis, and qRT‐PCR.ResultsmiR‐20a‐5p was remarkably elevated in PASMCs of PAH mice and human PASMCs treated by hypoxia, while ABCA1 was remarkably decreased. After transfection of miR‐20a‐5p mimics, PASMCs proliferation and migration were promoted and PASMCs apoptosis was suppressed. ABCA1 was confirmed to be a target of miR‐20a‐5p and restoration of ABCA1 reversed the function of miR‐20a‐5p.ConclusionmiR‐20a‐5p enhances the proliferation and migration of PASMCs to promote the development of PAH via targeting ABCA1.
Highly dispersible Fe3O4‐Ag@OPO(OH)2 nanocomposites as a novel eco‐friendly magnetic retrievable catalyst for the reduction of p‐nitrophenol
Authors: Hojjati Mahsa , Soleimani Esmaiel 
Published: July 2020

AbstractIn this study, Fe3O4‐Ag@OPO(OH)2 nanocomposites (NCs) were synthesized using Camellia sinensis leaf extract. This herbal extract was utilized to reduce silver(I) ions to silver nanoparticles (NPs) on the surface of magnetite. The solution color changes from bright to dark, along with the new absorption band at λmax 410 nm confirms the deposition of nanosilver on the magnetite surface. The X‐ray powder diffraction (XRD) analysis was used to characterize the diffraction patterns of silver and magnetite phases and to calculate the average size of the crystallites being 21 nm. The fourier transform infrared (FT‐IR) spectra of green prepared of Fe3O4‐Ag@OPO(OH)2 NCs illustrated the functional group peaks of flavonoids and other phenolic compounds. The energy dispersive X‐ray (EDS) spectra of Fe3O4‐Ag@OPO(OH)2 NCs proved the presence of localized elements, which are incorporated into prepared NCs containing Ag, Fe, O, and P. The vibrating‐sample magnetometer (VSM) analysis indicated that the magnetic nature of Fe3O4‐Ag@OPO(OH)2 NCs is less than Fe3O4 NPs, which is attributed to nonmagnetic nature of Ag NPs and covering the magnetite surface by layers of silver and phosphate. The eco‐friendly synthesized Fe3O4‐Ag@OPO(OH)2 NCs exhibited high catalytic activity toward reducing p‐nitrophenol.
Downregulation of miR‐1225‐5p is pivotal for proliferation, invasion, and migration of HCC cells through NFκB regulation
Authors: Liu Lin , Zhang Weiguo , Hu Yujing , Ma Liangliang , Xu Xiangsu 
Published: July 2020

AbstractBackgroundAs one of the most frequently seen malignancies, hepatocellular carcinoma (HCC) serves as the second largest contributor to malignancy‐specific mortality worldwide. MicroRNA‐1225‐5p (miR‐1225) exerts an essential impact on the growth and metastasis of many malignancies. However, the contribution of miR‐125 to HCC and the molecular mechanism of cancer cell viability and apoptosis are still unclear. We focused our research on exploring the function and molecular mechanism of miR‐1225 in regulating HCC cell growth, migration, and invasion.MaterialQuantitative PCR data showed that miR‐1225 expression was repressed in HCC cell lines and in the tissues of HCC patients, compared to that in normal human hepatic cells and tissues. Transfection of a miR‐1225 mimic inhibited cell viability and proliferation as indicated by CCK‐8 staining and MTT assay. Transwell invasion, wound healing assay, and Western blotting were performed to assess whether miR‐1225 repressed the metastasis and invasion of HCC cells, and decreased matrix metalloproteinase 9 (MMP9) expression. Further bioinformatic prediction and dual‐luciferase reporter assay suggested that miR‐1225 targeted the 3′‐UTR of NFκB p65.ResultsOverexpression of p65 protein counteracted the repressive impact of miR‐1225 on invasion, migration, and proliferation of HCC cells.ConclusionThis research provided new evidences that miR‐1225 inhibits the viability, migration, and invasion of HCC cells by downregulation of p65.
Can mentor organizations impact mentor outcomes? Assessing organizational norms on mentor intent to stay and willingness to “go the extra mile”
Authors: Ellison Rachael L. , Cory Molly , Horwath Jordan , Barnett Alexandra , Huppert Elizabeth 
Published: July 2020

AbstractThe current study explores how organizational norms within mentoring organizations predict mentor outcomes over and above individual mentor characteristics. Specifically, this study examines whether mean levels (as an indicator of organizational norms) of mentors' perceptions of their relationship quality with mentees' families predict mentor satisfaction, mentor intent to stay and mentor extra‐role prosocial behavior over and above individual mentor perception of their relationship quality with mentees' families. Multilevel modeling was used to assess 204 mentors nested within 37 mentoring organizations. The current study found that mentor organization averages of perceived relationship quality with mentees' families positively predicted mentor extra‐role prosocial behavior over and above the individual mentor perceptions of relationship quality with mentees' families. Additionally, organizational averages negatively predicted mentor intent to stay, while individual mentor perceptions positively predicted mentor intent to stay. Results have implications for mentoring organizations to create organizational norms that reduce burnout, increase continuity of mentor relationships, and help mentors go above and beyond on behalf of their mentees and mentoring organization.
Parent–child relationships in Latinx families: The role of immigrant concentration, residential stability, and generational status
Authors: Riina Elizabeth M. , Lippert Adam M. 
Published: July 2020

AbstractPrior research links neighborhood immigrant concentration and residential stability to individual health outcomes. It remains unclear how neighborhood social organization extend to family functioning among immigrant families. Expanding the scope of research on neighborhoods and family relationships, this study draws on data from 1417 Latinx families (Myouth age = 10.19 years, SDyouth age = 3.34) to: (1) examine associations between neighborhood immigrant concentration, residential stability, and parent–child warmth and conflict in Latinx families, and (2) test whether associations vary by family generational status. Multilevel mod3els revealed that associations between immigrant concentration and parent–child relationships were contingent on residential stability, and that the association differed according to generational status. In general, stable neighborhoods with larger immigrant populations were associated with more warmth and less conflict for first‐ and second‐generation families relative to foreign‐born families. Discussion centers on the conditional role of neighborhood structural characteristics for family relationships among Latinx families and advances directions for future research on neighborhoods and families.
Helicobacter pylori promote inflammation and host defense through the cagA‐dependent activation of mTORC1
Authors: Feng Guang‐Jing , Chen Yi , Li Ke 
Published: July 2020

AbstractMechanistic target of rapamycin complex 1 (mTORC1) functions as regulating different cellular processes, including cell growth, proliferation, motility, survival, metabolism, autophagy, and protein transcription. Recently, it also found to be associated with many infections and inflammatory diseases, playing complex roles in pathogens growth and inflammation regulation. However, the regulation mechanism of mTORC1 in gastric epithelial cells and its role in Helicobacter pylori (H. pylori) infection and related gastritis remain unclear. Here, we identified that the phosphorylation of mechanistic target of rapamycin (mTOR) and the expression of DEP domain‐containing mTOR‐interacting protein (DEPTOR) was increased in gastric mucosa of H. pylori‐infected patients and mice, as well as in H. pylori‐infected gastric epithelial cells, which were largely depended on H. pylori cagA. The expression of DEPTOR was regulated via mTORC1, but, in turn, inhibited mTORC1. Knockdown mTOR significantly decreased expression and secretion of cytokines tumor necrosis factor‐α, interleukin‐1β, and interleukin‐6, chemokines CCL7 and CXCL16, and antimicrobial peptide LL37 in vitro, while knockdown DEPTOR had the opposite effect. Similar observations were made using mTOR knockout (KO) mice in vivo, moreover. The gastric inflammation was attenuated, while the bacterial burden was increased in mTOR KO mice during H. pylori infection. These findings supported H. pylori promote gastritis and inhibit bacterial colonization through the cagA‐dependent activation of mTORC1.
Surface runoff and nutrient dynamics in cover crop–soybean systems in the Upper Midwest
Authors: Weyers Sharon L. , Gesch Russ W. , Forcella Frank , Eberle Carrie A. , Thom Matthew D. , Matthees Heather L. , Ott Matthew , Feyereisen Gary W. , Strock Jeffrey S. 
Published: July 2020

AbstractRelay‐cropping of the novel oilseeds winter camelina (Camelina sativa L.) and pennycress (Thlaspi arvense L.) with short‐season crops such as soybean [Glycine max (L.) Merr.] can provide economic and environmental incentives for adopting winter cover crop practices in the U.S. Upper Midwest. However, their ability to reduce nutrient loss in surface runoff is unknown. Accordingly, surface runoff and quality were evaluated during three seasonal phases (cover, intercrop, and soybean) over 2 yr in four cover crop–soybean treatments (pennycress, winter camelina, forage radish [Raphanus sativus L.], and winter rye [Secale cereale L.]) compared with no‐till and chisel‐till fallow treatments. Runoff was collected with Gerlach troughs and assessed for concentrations and loads of NO3−–N, total mineral N, soluble reactive P (SRP), and total suspended solids (TSS). Cumulative runoff and nutrient loads were greater during the winter cover phase because of increased snow melt and freeze–thaw released nutrients from living vegetation. In contrast, cumulative TSS was greater during intercrop and soybean phases due to high‐intensity rainfall events with an open soybean canopy. Average TSS loads during the intercrop phase were reduced by 75% in pennycress compared with fallow and radish treatments. During the soybean phase, average TSS, total mineral N, and SRP loads were generally elevated in cover crop treatments compared with no‐till. Overwintering cover crops may contribute to mobility of nutrients solubilized from living or decomposing vegetation; however, this was balanced by their potential to reduce runoff and TSS during high‐intensity spring rains.
More than just a common cold: Endemic coronaviruses OC43, HKU1, NL63, and 229E associated with severe acute respiratory infection and fatality cases among healthy adults
Authors: Veiga Ana B. Gorini da , Martins Letícia G. , Riediger Irina , Mazetto Alix , Debur Maria do Carmo , Gregianini Tatiana S. 
Published: July 2020

AbstractRespiratory viral infection can cause severe disease and hospitalization, especially among children, the elderly, and patients with comorbidities. In Brazil, the official surveillance system of severe acute respiratory infection (SARI) investigates influenza A (IAV) and B (IBV) viruses, respiratory syncytial virus (RSV), adenovirus (HAdV), and parainfluenza viruses (hPIV 1–3). In Rio Grande do Sul (RS), Brazil, many fatalities associated with SARI between 2013 and 2017 occurred among patients without underlying diseases and for whom the causative agent had not been identified using official protocols. This cross‐sectional study analyzed the presence of coronaviruses (HCoV), bocavirus (HBoV), metapneumovirus (hMPV), and rhinovirus in patients who died of SARI despite not having comorbidities, and that were negative for IAV, IBV, RSV, HAdV, and hPIV. Nasopharyngeal aspirates/swabs from patients were used for nucleic acid extraction. The presence of HCoVs OC43, HKU1, NL63, and 229E; HBoV; hMPV; and rhinovirus was assessed by quantitative reverse transcription‐polymerase chain reaction. Clinical data were also analyzed. Between 2013 and 2017, 16 225 cases of SARI were reported in RS; 9.8% of the patients died; 20% of all fatal cases were patients without comorbidities and for whom no pathogen was detected using standard protocols. Analysis of 271 of these cases identified HCoV in nine cases; HBoV, hMPV, and rhinovirus were detected in 3, 3, and 10 cases, respectively. Of note, patients infected with HCoV were adults. Results reinforce the importance of including coronaviruses in diagnostic panels used by official surveillance systems because besides their pandemic potential, endemic HCoVs are associated to severe disease in healthy adults.
High prevalence of SARS‐CoV‐2 and influenza A virus (H1N1) coinfection in dead patients in Northeastern Iran
Authors: Hashemi Seyed A. , Safamanesh Saghar , Ghasemzadeh‐moghaddam Hamed , Ghafouri Majid , Azimian Amir 
Published: July 2020

AbstractIn the last months of 2019, an outbreak of fatal respiratory disease started in Wuhan, China, and quickly spread to other parts of the world. It was named COVID‐19, and to date, thousands of cases of infection and death are reported worldwide. This disease is associated with a wide range of symptoms, which makes accurate diagnosis of it difficult. During previous severe acute respiratory syndrome (SARS) pandemic in 2003, researchers found that the patients with fever, cough, or sore throat had a 5% influenza virus‐positive rate. This finding made us think that the wide range of symptoms and also relatively high prevalence of death in our patients may be due to the coinfection with other viruses. Thus, we evaluated the coinfection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) with other respiratory viruses in dead patients in North Khorasan. We evaluated the presence of influenza A/B virus, human metapneumovirus, bocavirus, adenovirus, respiratory syncytial virus (RSV), and parainfluenza viruses in 105 SARS‐CoV‐2 positive dead patients, using polymerase chain reaction (PCR) and reverse transcription PCR tests. We found coinfection with influenza virus in 22.3%, RSV, and bocavirus in 9.7%, parainfluenza viruses in 3.9%, human metapneumovirus in 2.9%, and finally adenovirus in 1.9% of SARS‐CoV‐2 positive dead cases. Our findings highlight a high prevalence of coinfection with influenza A virus and the monopoly of coinfection with Human metapneumovirus in children.
THE SLINGSHOT METHOD: BECOMING A LEADER WHO MATTERS IN TIMES OF ADVERSITY
Authors: Bhandari Rahul 
Published: July 2020

AbstractThis article explains the Slingshot Method, a way to make sense of, and then intentionally design and create lives of significance. I drew deeply from my own career as a corporate executive, entrepreneur and investor including as founding team member of Accenture Ventures, a $1 Billion corporate ventures fund at Accenture; and as President, CEO and Chairman of a data analytics company, where I led large change management engagement including for the U.S Department of Defense. I also led global Mergers and Acquisitions for Thomson Reuters, and the development of emerging technologies at Oracle and Intelsat.First part of the article describes the journey from surviving to thriving, with four contexts of Survival, Security, Success and Significance. The second part describes Pulling Back, Propelling Forward: Using the Slingshot Method to Identify Life Goals. This arc from survival to security and success, and eventually significance, isn't some rare phenomena reserved for the privileged few. In fact, evolutionary psychologists believe this ultimate development from the primary concern for survival (a selfish activity) to significance (an altruistic activity) is built into our genes. The article draws on research from psychologists including Abraham Maslow, Login George, Crystal Park, and Dacher Keltner, as well as business professor Alexander Horniman.
Effect of Sample Duration on Catch Rate and Size Structure Data for Blue Catfish Collected by Low-Frequency Electrofishing
Authors: Shoup Daniel E. , Bodine Kristopher A. 
Published: July 2020

Abstract
Although low-frequency, pulsed-DC electrofishing is considered the most effective method for sampling Blue Catfish Ictalurus furcatus in reservoirs, efforts to improve sampling efficiency have not been fully explored. Optimizing sampling duration can reduce cost and effort if shorter sample times still produce precision similar to that of longer samples. We compared several catch rate and size structure metrics from the first and second 5-min intervals of 10-min low-frequency electrofishing samples for Blue Catfish in three Oklahoma reservoirs (N = 40 sites total). The total Blue Catfish CPUE (CPUETotal), CPUE of preferred-length fish (&gt;760 mm; CPUE760), mean TL, proportional size distribution (PSD), and PSD of preferred-length fish did not significantly differ between sampling intervals. One reservoir had a higher proportion of 200–299-mm fish in the second 5-min interval; however, no other differences in length frequency or other size metrics were detected between the first and second 5-min intervals in any reservoir. Sampling precision met the target level (relative standard error = 20) for CPUETotal but not CPUE760 during both the first 5 min and the full 10-min sample at all reservoirs. Monte Carlo simulation indicated that 10 samples would be needed to achieve the target precision (relative standard error ≤ 20) for CPUETotal and 38–65 samples would be needed for precise CPUE760 metrics regardless of sample duration. In fisheries with moderate catch rates like those we observed (150–500 fish/h), we recommend short-duration (i.e., 5-min) sampling because data of the same quality can be collected with reduced cost and effort. For situations in which sampling precision is low (e.g., CPUE760), we recommend increasing the number of replicate 5-min samples. Using shorter sample durations provides a method for managers to obtain quality population data more efficiently, allowing time for other worthwhile management activities that would otherwise not be possible.
Arabic language validation of TANGO questionnaire for nocturia: A translation into the Arabian Peninsula dialect
Authors: Altaweel Waleed , Alrumayyan Majed , Seyam Raouf 
Published: July 2020

AbstractAimsTargeting the Aetiology of Nocturia to Guide Outcomes (TANGO) is a validated English language questionnaire to identify underlying factors leading to nocturia. We set out to provide a culturally correct Arabic version of the TANGO questionnaire in Saudi Arabia.MethodsThe English version underwent forwarded and backward translation. The final version was administered to native Arabic speaking hospital employees and patients. Test‐retest reliability and the clarity of questions were determined by Kappa and Cronbach's α statistics.ResultsThe translation: The median Likert score for translated questions was ≥4 with a Cronbach's α .865. Test‐retest reliability: Thirty employees answered the questionnaire. The median, standard deviation (SD), and range were age 29.0 ± 11.0 years (20‐55), and nocturia 1.0 ± 0.96 episodes per night (0‐4). Three questions had identical constant answers and no Kappa values were computed. Based on prevalence‐adjusted bias‐adjusted Kappa (PABAK) values, 10 questions had a “very good” agreement, eight questions had a “good” agreement, and one item had a “moderate” agreement. The range of PABAK was 0.53 to 1, P < .01.Clarity of questions: The employees had a median Likert score of 5.0 with a Cronbach's α .961. Thirty‐two patients answered the questionnaire. The median, SD, and range were age 40.0 ± 15.4 years (17‐79), and nocturia 1.0 ± 1.6 episodes per night (0‐6). The median item score on the Likert scale was 5.0, with a Cronbach's α .909. A total of 29 (90.6%) patients stated that the questions were clear or needed no help to fill in the questionnaire.ConclusionThe Arabic language translation of TANGO questionnaire is a valid tool in the dialect of Saudi Arabia.
Outcomes of transurethral resection of the prostate in unobstructed patients with concomitant detrusor underactivity
Authors: Rubilotta Emanuele , Balzarro Matteo , Gubbiotti Marilena , Antonelli Alessandro 
Published: July 2020

AbstractAimsThe aim of the study was to evaluate the transurethral resection of the prostate (TURP) outcomes of unobstructed patients with detrusor underactivity (DUA), comparing the surgical results between obstructed and unobstructed males with concomitant DUA, at midterm follow‐up.MethodsThis was an observational, prospective, comparative, nonrandomized study. Candidates to TURP underwent preoperative urodynamics (UD), with a diagnosis of DUA, were divided in two cohorts: Group A unobstructed men, group B males with bladder outlet obstruction (BOO). Males were evaluated yearly with uroflowmetry (UF), post‐void residual (PVR), and bladder voiding efficiency (BVE), International Prostate Symptom Score (IPSS) questionnaire, visual analogic scale (VAS) for subjective assessment of the quality of life. The degree of the variation of maximum flow rate (Qmax), PVR, BVE, IPSS, VAS between baseline and follow‐up (Δ) was evaluated.ResultsPatients in group A were 28 and in group B 23. Overall patient's mean ± SD age was 63.37 ± 12.41 years. Preoperative urodynamics characteristics: mean bladder contractility index (BCI) of 61.15 and 76.25 in group A and B, respectively; mean bladder outlet obstruction index (BOOI) of 17.25 and 50.15 in group A and group B, respectively. After surgery, overall patient group, group A, and group B showed a statistical improvement in IPSS score (P < .0001), Qmax (P < .0001), PVR (P < .0008), BVE (P < .03) and VAS (P < .0001).ConclusionsBOO had an important impact on the degree of improvement of Qmax and PVR/BVE, while had a poor influence on lower urinary tract symptoms amelioration. The most relevant outcomes were found when BOO was associated with DUA, which was not a contraindication to surgery.