bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary appendiceal adenocarcinoma presenting as advanced ovarian cancer: a case report
Authors: Cheng Kung-Chuan , Lee Ko-Chao , Chen Hong-Hwa , Ou YC 
Published: September 2020

Abstract
BackgroundAlthough Primary appendiceal malignancies are rare accounting for less than 1% of all gastrointestinal tumors, the incidence of ovarian metastasis in appendiceal adenocarcinoma is encountered in 16.7 to 37% of all ovarian malignancy. The diagnosis is usually made after an exploratory laparotomy. Most of these tumors present with nonspecific symptoms, such as abdominal pain, abdominal fullness and huge pelvic mass, mimicking advanced stage ovarian cancer.Case PresentationWe described two menopausal women who were referred to our institution under the diagnostic impression of advanced stage of primary ovarian cancer. However, both patients were eventually found to have primary appendiceal adenocarcinoma with ovarian metastasis at laparotomy. Initial symptoms, intraoperative finding, pathology finding, and postoperative clinical courses were documented.ConclusionsComparing with primary ovarian tumor, ovarian metastasis is relatively rare. The clinical presentation can be misleading and the differential diagnoses of primary appendiceal cancer should be considered when preoperative workup is planned.
Genome-Wide Association Studies for Immunoglobulin Concentrations in Colostrum and Serum in Chinese Holstein
Authors: Lin Shan , Ke Cuncun , Liu Lin , Gao Yahui , Xu Lingna , Han Bo , Zhao Yaofeng , Zhang Shengli , Sun Dongxiao 
Published: September 2020

Abstract
BackgroundThe early death and health problems of calves caused substantial economic losses in dairy industry. As the immune system has not been fully developed in the neonates, transport of passive immune substance such as immunoglobulins (Ig) from mothers to newborn calves is essential in protecting neonates from infections in their early life. Therefore, concentrations of immunoglobulins in the colostrum and serum of dairy cows are critical traits when estimating potential disease resistance of its offspring. ResultsColostrum, blood and hair follicle samples were collected from the 620 Chinese Holstein cows within 24 hours after calving. The concentration of total IgG, IgG1, IgG2, IgA and IgM in both colostrum and serum were detected via ELISA methods. With GCTA software, genome-wide association studies (GWASs) were performed with 88,934 SNPs genotyped by using Illumina 50K (54,609 SNPs) and GeneSeek 150K (140,668 SNPs) chips in which 50K chip were imputed to 150K SNPs with BEAGLE. As a result, 20, 1 and 4 significant SNPs were detected associated with the concentrations of IgG2, IgA and IgM at genome-wide level (P < 3.16E–6); 11, 11, 35, 11 and 10 significant SNPs were identified associated with total IgG, IgG1, IgG2, IgA and IgM at suggestive level (P < 6.32E–5). Such SNPs were located in or proximate to (±1 Mb) 1,083 genes, which were functionally implicated in biological processes and pathways, such as immune response, negative regulation of immunoglobulin secretion, Fc-epsilon receptor and NF-kappaB signaling pathways. By combining the biological functions and the known QTL data for immune traits in bovine, 21 promising candidate functional genes were identified for immunoglobulins concentrations in colostrum and serum in dairy cattle, they were ABR, TIMM22, CRK, MYO1C, RILP, SERPINF2, AKT1, BCL11B, HHIPL1, DYNC1H1, HSP90AA1, TRAF3, KLC1, IL6, PYCARD, ITGAM, TGFB1I1, GUSB, CRCP, RABGEF1 and SBDS.ConclusionsIn this study, we identified 21 candidate genes related to concentrations of immunoglobulins in colostrum and serum in dairy cattle by performing GWASs. Our findings provide a groundwork for unraveling the key genes and causal mutations affecting immunoglobulins levels in colostrum and important information for genetic improvement of such traits in dairy cattle.
Hsa_circ_0001944 promotes the growth and metastasis in bladder cancer cells by acting as a competitive endogenous RNA for miR-548
Authors: Jin Mingming , Lu Shengjie , Wu Yue , Yang Chen , Shi Chunzi , Wang Yanqiu , Huang Gang 
Published: September 2020

Abstract
Background: Bladder cancer (BC) is a common genitourinary malignancy worldwide. Circular RNAs (circRNAs) participate in cancer development, including BC; thus, the roles of circRNAs in this process have attracted significant attention. Methods: In this study, high-throughput sequencing was used to analyze circRNA expression profiles in BC tissues. We performed RT-qPCR to determine hsa_circ_0001944 expression in BC tissues. We used fluorescence in situ hybridization (FISH) to detect hsa_circ_0001944 expression and hsa_circ_0001944 subcellular localization in BC tissues. hsa_circ_0001944 expression in BC cells was selectively regulated. We employed CCK8, transwell, and wound healing assays to monitor cell proliferation, invasion, and migration, respectively. We employed the dual-luciferase reporter and RNA pulldown assays to verify the relationships among hsa_circ_0001944, miR-548, and PROK2. We examined the effects of hsa_circ_0001944 on BC cell metastasis and proliferation in vivo using a subcutaneous xenograft model and an intravenous tail injection model in nude mice. Results: The results showed that hsa_circ_0001944 expression was significantly increased in BC samples. Furthermore, high hsa_circ_0001944 expression predicted unfavorable prognoses in BC. Functional assays validated that downregulating hsa_circ_0001944 decreased BC invasion and proliferation in vivo and in vitro. Further studies showed that hsa_circ_0001944 expression promoted BC progression via sponging miR-548 and enhancing PROK2 expression. Luciferase reporter experiments validated the interactions between hsa_circ_0001944, miR-548, and PROK2. This study also found that downregulating miR-548 or overexpressing PROK2 restored BC cell invasion and proliferation after silencing hsa_circ_0001944. Conclusions: Taken together, we found that hsa_circ_0001944 is a tumor-promoting circRNA in BC that functions as a competing endogenous RNA to regulate PROK2 expression via sponging miR-548.
Ecological Benefit Analysis of Restoration of Degraded Environment by Artificial Tamarix-Cistanche
Authors: Jiang Lei , Wang Ying , He Liang 
Published: September 2020

Abstract
The Hotan Region in xinjiang, China is a typical arid area. Natural factors determine that the ecological stability of the area is poor, easy to be damaged and difficult to recover. In order to improve the local ecological environment, this study explored an ecology restoration model with the artificial Tamarix-Cistanche. After the long-term monitoring and comparison at four test sites, it was found that this model also increased the per capita income, reduced the poverty in local farmers, and solved the problem of no direct economic benefit from foresting, as well as the following ecological benefits (1) improving the soil properties, and increasing its powder content and fertility, (2) improving the regional microclimate, reducing the daily temperature and relative humidity ranges, and reducing the regional wind speed, (3) restoring the biodiversity, increasing the vegetation coverage and the amount of animals and plants, and enhancing the water and fertility retention of the soil.
Identification of Globin Switching-Related Genes and RNAs via Transcriptomic Profiling of Nucleated Red Blood Cells Isolated from the Cord Blood of Preterm and Full-Term Newborns
Authors: Han Yuanyuan , Huang Ling , Zhou Man , Gong Shangjin , Zhang Zhaojun , Jin Tingting , Liu Juan , Hu Li , Zhang Wenqiu , Fang Xiangdong , Jia Yankai , Huang Shengwen 
Published: September 2020

Abstract
Background: β-thalassemia and sickle cell disease (SCD) are chronic hemolytic anemias caused by mutation or deletion of the β-globin gene. Reactivation of fetal γ-globin gene (HBG) expression can ameliorate the clinical course of β-hemoglobinopathies. Our current understanding of the globin switching mechanism is not comprehensive, and research into the roles of long non-coding RNAs (lncRNAs) in this process remains limited. Results: We collected umbilical cord blood samples from preterm and full-term infants, from which we enriched and isolated nucleated red blood cells (NRBCs) for transcriptome sequencing. We identified mRNAs, lncRNAs, and microRNAs that were differentially expressed in the preterm and full-term samples. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis revealed the biological significance of differentially expressed mRNAs and the targets of differentially expressed lncRNAs and microRNAs. Our analyses revealed that the Fanconi anemia pathway may be involved in the regulation of globin switching at birth. Using quantitative polymerase chain reaction, we confirm that BCL11A is more highly expressed in full-term samples than in preterm samples and is statistically differentially expressed based on biological sex. Results of RNA sequencing and quantitative polymerase chain reaction demonstrated that the lncRNA H19 was expressed at a statistically lower level in full-term samples than in preterm samples.Conclusions: Our results suggest that the Fanconi anemia pathway and the H19/let-7/LIN28B axis may be involved in globin switching in umbilical cord blood. The differentially expressed transcripts and their related pathways may constitute novel therapeutic targets for β-thalassemia.
Whole Genomic Comparative Analysis of Streptococcus Pneumoniae Serotype 1 Isolates Causing Invasive and Non-Invasive Infections Among Children Under 5 years in Casablanca, Morocco
Authors: Nzoyikorera Néhémie , Diawara Idrissa , Fresia Pablo , Maaloum Fakhreddine , Katfy Khalid , Nayme Kaotar , Maaloum Mossaab , Cornick Jennifer , Chaguza Chrispin , Timinouni Mohammed , Belabess Houria , Zerouali Khalid , Elmdaghri Naima 
Published: September 2020

Abstract
Background: Streptococcus pneumoniae serotype 1 remains a leading cause of invasive pneumococcal diseases, even in countries with PCV-10/PCV-13 vaccine implementation. The main objective of this study, which is part of the Pneumococcal African Genome project (PAGe), was to determine the phylogenetic relationships of serotype 1 isolates recovered from children patients in Casablanca (Morocco), compared to these from other African countries; and to investigate the contribution of accessory genes and recombination events to the genetic diversity of this serotype.Results: The genome average size of the six-pneumococcus serotype 1 from Casablanca was 2,227,119 bp, and the average content of coding sequences was 2113, ranging from 2041 to 2161. Pangenome analysis of the 80 genomes used in this study revealed 1685 core genes and 1805 accessory genes. The phylogenetic tree based on core genes and the hierarchical bayesian clustering analysis revealed five sublineages with a phylogeographic structure by country. The Moroccan strains cluster in two different lineages, the five invasive strains clusters altogether in a divergent clade distantly related to the non-invasive strain, that cluster with all the serotype 1 genomes from Africa.Conclusions: The whole genome sequencing provides increased resolution analysis of the highly virulent serotype 1 in Casablanca, Morocco. Our results are concordant with previous works, showing that the phylogeography of S. pneumoniae serotype 1 is structured by country, and despite the small size (six isolates) of the Moroccan sample, our analysis shows the genetic cohesion of the Moroccan isolates.
Acute Abdomen in Adult Revealing Unusual Complicated Epiploic Appendagitis: A Case Report
Authors: charifi yahya , Youssef alaoui lamrani My , Laila Chbani , Meryem Boubbou , Mustapha Maaroufi , Alami Badreddine 
Published: September 2020

Abstract
BACKGROUND Epiploic appendagitis is a torsion of fatty appendages of ligamentum trees (1), its a rare cause of abdominal pain that usually manifests by right or left iliac fossa pain, reminding of appendicitis, diverticulitis or ischemia of the omentum. CASE PRESENTATION We report the case of a 56 years old male patient admitted for an epigastric pain and a right-upper quadrant abdominal pain, for whom he underwent an abdominal ultrasound and a CT-scan who shown an abscessed mass under colic transverse to the unusual seat. CT scan is still the key modality for diagnosis, avoiding unnecessary surgery. Complications of primary epiploic appendagitis have rarely been described in the literature. Another more rare complication is appendagitis with associated abscess, in this condition, we must also think about complicated diverticulitis. The treatment of uncomplicated forms is generally conservative, however, some authors have suggested a surgical method . For therapeutic management of its complication, there has been much interest in the use of minimally invasive techniques such as percutaneous drainage to minimize the morbidity and mortality that is associated with surgery. CONCLUSION The diagnosis of epiploic appendagitis is still uncommon, CT scan, can eliminate other diagnosis of acute abdominal pain such as diverticulitis and appendicitis. It also allows the diagnosis of the rare complicated forms such as abscesses associated with epiploic appendagitis. The treatment of typical forms is usually conservative, while the complicated form requires surgery because of the potential associated pathology.
Infection and transmission of the facultative endosymbiont Arsenophonus in the spider Pardosa pseudoannulata
Authors: Jiang Hanqi , Ding Yu , Zhao Dongxiao , Liu Xiangdong , Guo Huifang 
Published: September 2020

Abstract
Background The infection of insect pests and their parasitoids with the facultative endosymbiont Arsenophonus has been well studied, whereas the infection of the predators of insect pests with this symbiont is less well understood. We studied this symbiont in the spider Pardosa pseudoannulata, an important predator of rice planthoppers, collected from rice paddies in 8 geographical regions in China.Results Diagnostic PCR showed that Arsenophonus was present in 4 of the 8 sampled populations of spiders. In these 4 populations, the infection incidence was between 2.1% and 16.7% and significantly differed between the Lishui population and the other three populations. Investigation of symbiont infection in both females and their offspring indicated that Arsenophonus can be vertically transmitted in P. pseudoannulata; the average transmission rate was approximately 25%. Remarkably, Arsenophonus was present in approximately 11% of the offspring of uninfected females, indicating that nonmaternal transmission occurs in this spider.Conclusions Populations of the spider P. pseudoannulata are infected at low rates with the symbiont Arsenophonus. This symbiont can be vertically transmitted from female spiders to offspring but may also be transmitted by male spiders or other vectors. Arsenophonus populations from different geographical regions exhibit genetic differentiation.
Prediction of Enhancer-Promoter Interactions Using the Cross-Cell Information and Domain Adversarial Neural Network
Authors: Jing Fang , Zhang Shao-Wu , Zhang Shihua 
Published: September 2020

Abstract
Background: Enhancer-promoter interactions (EPIs) play key roles in transcriptional regulation and disease progression. Although several computational methods have been developed to predict such interactions, their performances are not satisfactory when training and testing data from different cell lines. Currently, it is still unclear what extent EPI prediction across cell lines can be made based on sequence-level information.Results: In this work, we present a novel Sequence-based method (called SEPT) to predict the Enhancer-Promoter interactions in the new cell line by using the cross-cell information and Transfer learning. SEPT first leans the features of enhancer and promoter from DNA sequences with convolutional neural network (CNN), then designing the gradient reversal layer of transfer leaning to reduce the cell line specific features meanwhile retaining the features associated with EPIs. When the locations of enhancers and promoters are provided in new cell line, SEPT can successfully recognize EPIs in this new cell line based on labeled data of other cell lines. The experiment results show that SEPT can effectively learn the latent import EPIs-related features between cell lines and achieves the best prediction performance in terms of AUC (the area under the receiver operating curves).Conclusion: SEPT is an effective method for predicting EPIs in the new cell line. Domain adversarial architecture of transfer leaning used in SEPT can learn the latent EPI features shared among cell lines from all other existing labeled data. It can be expected that SEPT will be of interest to researchers concerned with biological interaction prediction.
Prior context influences motor brain areas in an auditory oddball task and prefrontal cortex multitasking modelling
Authors: Mugruza-Vassallo Carlos A. , Potter Douglas D. , Tsiora Stamatina , Macfarlane Jennifer , Maxwell Adele 
Published: September 2020

Abstract
In this study, the relationship of orienting of attention, motor control and the Stimulus- (SDN) and Goal-Driven Network (GDN) was explored through an innovative method for fMRI analysis considering all voxels after every type condition. The task consisted of four conditions: standard target (G), novel (N), neutral (Z) and noisy target (NG). First running average reaction times of each condition was made. In the second level analysis, ‘distracted’ participants evoke brain activations and differences in both hemispheres neural network for selective attention as previously reported while the participants, as a whole, demonstrated mainly left cortical and subcortical activations. A context analysis was run in the behaviourally distracted participant group contrasting the trials immediately prior to the G trials, namely one of the Z, N or NG conditions. Results showed different prefrontal activations were evoked dependent on prior context in auditory modality with recruiting from 1 to 10 brain prefrontal areas. The more motor response and influence of the previous novel stimulus the more prefrontal areas, which extend hierarchical studies of prefrontal control of attention and explain better how the auditory processing interferes with movement. Also, it was addressed how subcortical loops and model of previous motor response affected the signal processing the novel stimulus, lateralized or simultaneously, with the Target. This model makes the first grasp to understand how auditory stimulus is changing motor responses in a kind of self-induced motor response. Moreover, current BCI works address some multimodal stimulus-driven systems.
A Clinical Practice Guideline Appraisal for Appropriate Use of Echocardiography in Adult Infective Endocarditis—When to and by Which Mode to Perform an Echo?
Authors: Xie Peihan , Zhuang Xiaodong , Liu Menghui , Zhang Shaozhao , Liu Jia , Liu Donghong , Liao Xinxue 
Published: September 2020

Abstract
BackgroundEchocardiography (Echo) is the primary imaging modality of infective endocarditis (IE). The recommendations on timing and mode selection of transesophageal echocardiography (TEE) and transthoracic echocardiography (TTE) are not fully in agreement among different guidelines, which can be confusing for clinical decision makers. Thus, we aimed to appraise the quality of recommendations by appraising the quality of guidelines. MethodsA search of guidelines published in English during 2007 to 2020 which contains recommendations of appropriate use of Echo in IE adult patients has been conducted. PPRAISAL OF GUIDELINES FOR RESEARCH & EVALUATION II (AGREE II) instrument was applied independently by two reviews to assess the integrated quality of identified guidelines. The recommendations of concern were extracted from related chapters.ResultsA total of 9 guidelines meet the criteria with AGREE II score ranging from 36% to 79%, the domain of “stakeholder involvement” got the lowest score. According The most debatable issue is that under what circumstances has the repeated TEE for an initial positive TTE been necessary in suspected IE, the conflicting recommendations on it were presented along with relatively lower evidence level for hardly any related evidence based on.ConclusionsIn the 9 guidelines identified, the recommendations over the appropriate use of Echo are generally satisfying. Clinicians could take into account the guideline quality score when evaluating the recommendations for clinical decision making. More researches with high-grade evidence above the most controversial issues of whether a subsequent TEE is mandatory in uncomplicated native valve IE with an initial positive TTE are needed in the future.
Efficacy of tolvaptan on nephrotic patients with diuretic-resistant edema: a pilot study
Authors: Matsuura Ryo , Ohtake Takayasu , Mochida Yasuhiro , Ishioka Kunihiro , Moriya Hidekazu , Hidaka Sumi , Imamura Teruhiko , Kinugawa Koichiro , Kobayashi Shuzo 
Published: September 2020

Abstract
Background Tolvaptan (TLV), a vasopressin type 2 receptor antagonist, is an effective drug for heart failure without worsening renal function. However, the effect of TLV on nephrotic syndrome remains unknown. This study aims to assess the association between response to TLV and clinical parameters including pathological evaluation in nephrotic patients with diuretics-resistance.Methods For prospectively enrolled patients, TLV was added for 14 days after renal biopsy performed. We evaluated the effect of TLV on urine output (UO) and body weight (BW) and the correlation of UO change with BW, urine and blood measurements and pathological evaluation. Pathological evaluation included glomerular sclerosis, tubulointerstitial injury, interstitial fibrosis and the degree of aquaporin 2 (AQP2) positivity in collecting ducts.Results Ten nephrotic patients were enrolled. Two patients already received 140 and 160mg furosemide and others 40mg furosemide before TLV. After TLV administration, no patients showed worsening renal function or hypernatremia (>145 mEq/L) and two patients without any improvement of symptoms discontinued the trial. TLV significantly increased UO at day 1 and decreased BW overall. UO increase at day 1 was significantly correlated with BW decrease at the last follow-up. UO increase at day 1 was correlated only with AQP2 positivity in collecting ducts on biopsied kidney (r2=0.59, p=0.01) while other urine and blood measurements were not. Conclusions TLV was effective on nephrotic patients. UO increase by TLV was correlated with AQP2 positivity in the collecting ducts while biochemistry in urine and blood was not.