bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intra-Articular Opening Osteotomy Combined with Lateral Ligament Reconstruction for Varus Ankle Arthritis
Authors: Xu Yang , Li Xing-chen , Guo Chang-jun , Xu Xiang-yang 
Published: September 2020

Abstract
Background
One type of Takakura 3B ankle arthritis is varus talus with medial disital tibial platform erosion. Among these cases, the tibial anterior surface (TAS) angles are usually normal. The purpose of this study was to evaluate the therapeutic outcomes of intra-articular opening osteotomy combined with lateral ligament reconstruction for Takakura 3B ankle arthritis with medial disital tibial platform erosion.
Methods
From September 2009 to May 2016, 17 patients with Takakura 3B ankle arthritis were reviewed, including 3 male and 14 female patients. All underwent the operation of intra-articular opening osteotomy combined with lateral ligament reconstruction. All patients were available for analysis. The main outcome measurements included TT angle, AOFAS score, VAS score, SF-36 scale and AOS scale.
Results
All patients were followed for a mean follow-up of 87.2 months (range, 49 to 129 months). The VAS scale improved from 5.5 ± 1.6 to 2.3 ± 1.9. The mean AOFAS score improved from 47.7 ± 15.7 to 75.8 ± 12.0. The SF-36 scale improved from 41.6 ± 14.0 to 67.7 ± 14.6. The AOS improved from 60.9 ± 13.9 to 28.2 ± 17.7. The TT angle improved from 14.3 ± 5.0° to 5.3 ± 4.0°.
Conclusion
Intra-articular opening osteotomy combined with lateral ligament reconstruction is an effective method to treat varus ankle arthritis with medial disital tibial platform erosion.
Establishment and Application of Reverse Transcription Recombinase-aided Amplification as a Rapid Detection Method for SARS-COV-2
Authors: Ai Lele , Liu Wei , Ye Fuqiang , Ding Chenxi , Dai Han , Han Yifang , Zhu Changqiang , Lv Ruichen , Han Zhaojiu , Zhou Xiaobo , Qi Yong , Tan Weilong 
Published: September 2020

Abstract
Background: By the end of August 2020, >23 million cases and 800,000 deaths were attributed to SARS-CoV-2 in >200 countries. The improvement of simple, rapid, and efficient detection methods is of great significance for the early detection, timely isolation, and protection of susceptible populations. This study aimed to provide an alternative method for the rapid detection of viral nucleic acid.Methods: This study provided a rapid nucleic acid detection method mediated by recombinant enzyme based on the novel coronavirus (SARS-CoV-2). Primers and probes were designed based on the N gene sequence of coronavirus. The method was performed at 39 °C, the detection time was short (<20 min), and the detection limit was up to 101 copies/mL.Results: The primer-probe did not show any cross-reaction with adenovirus, Zika virus, influenza B virus, and chikungunya virus, with good specificity. A total of 106 clinical throat swab samples were compared by reverse transcription recombinase-aided amplification (RT-RAA) and commercial reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR); the results were identical.Conclusions: The novel coronavirus RT-RAA method established in this study had high sensitivity, strong specificity, simple operation, and fast detection speed, and hence, is suitable for the rapid detection of novel coronavirus under the current epidemic situation.
Prediction the burden of COVID-19 in Iran: Application of disability-adjusted life years (DALYs)
Authors: Mirzaee Negar , Barouni Mohsen , Goudarzi Reza , Jahani Yunes , Haghdoost AliAkbar , Gohari Milad Ahmadi , Nakhaeizadeh Mehran , McFarland Willi , Sharifi Hamid 
Published: September 2020

Abstract
Background The novel Coronavirus disease 2019 (COVID-19) rapidly became the world’s largest threat to health and the economy in recent times. Prediction of the COVID-19 pandemic’s full impact is a necessary gauge for future policy making, including resource allocation for prevention, mitigation, and control preparedness.Methods We used the extended form of the Susceptible-Exposed-Infected/Infectious-Recovered/Removed (SEIR) model to predict new cases and number of deaths associated to COVID-19. Data from the Ministry of Health and Medical Education of Iran provided relevant parameters for predicting disability-adjusted life years (DALYs). We conducted a review of the literature on COVID-19-like diseases to develop disability weights (DWs) and convened an expert panel to verify their applicability. Beta-PERT distributions were used to calculate DWs for age groups. The minimum and maximum values were 0 and 0.14 for mild to severe disability, respectively.Results The total DALYs for COVID-19 in Iran predicted by our model will be 973 per 100,000 populations from January, 2020 to January, 2021. Overall, 957 years per 100,000 will be from YLLs (98.4% of DALYs) and 16 will be from YLDs (1.6% of DALY). The total DALYs in men will be 1,082 years per 100,000 and 861 per 100,000 in women.Conclusions Our predictions of COVID-19 burden will be useful in determining health priorities and to appropriately allocate resources to prepare for future outbreaks of COVID-19 and similar diseases. We hope this study will contribute to evidence-based health policy making in Iran.
Global Knockdown of Glutamate Decarboxylase 67 Elicits Emotional and Auditory Abnormalities in Mice
Authors: Miyata Shigeo , Kakizaki Toshikazu , Fujihara Kazuyuki , Obinata Hideru , Hirano Touko , Nakai Junichi , Tanaka Mika , Itohara Shigeyoshi , Watanabe Masahiko , Tanaka Kenji F , Abe Manabu , Sakimura Kenji , Yanagawa Yuchio 
Published: September 2020

Abstract
Reduced expression of glutamate decarboxylase 67 (GAD67), encoded by the Gad1 gene, is a consistent finding in postmortem brains of patients with several psychiatric disorders, including schizophrenia, bipolar disorder and major depressive disorder. The dysfunction of GAD67 in the brain is implicated in the pathophysiology of these psychiatric disorders; however, the neurobiological consequences of GAD67 dysfunction in mature brains are not fully understood because the homozygous Gad1 knockout is lethal in newborn mice. We hypothesized that the tetracycline-controlled gene expression/suppression system could be applied to develop global GAD67 knockdown mice that would survive into adulthood. In addition, GAD67 knockdown mice would provide new insights into the neurobiological impact of GAD67 dysfunction. Here, we developed Gad1 tTA/STOP-tetO biallelic knock-in mice using Gad1 STOP-tetO and Gad1 tTA knock-in mice, and compared them with Gad1 +/+ mice. The expression level of GAD67 protein in brains of Gad1 tTA/STOP-tetO mice treated with doxycycline (Dox) was decreased by approximately 90%. The GABA content was also decreased in the brains of Dox-treated Gad1 tTA/STOP-tetO mice. In the open-field test, Dox-treated Gad1 tTA/STOP-tetO mice exhibited hyper-locomotor activity and decreased duration spent in the center region. In addition, acoustic startle responses were impaired in Dox-treated Gad1 tTA/STOP-tetO mice. These results suggest that global reduction in GAD67 elicits emotional and auditory abnormalities in mice. These GAD67 knockdown mice will be useful for elucidating the neurobiological mechanisms of emotional abnormalities, such as anxiety symptoms associated with psychiatric disorders.
Evaluating receiver contributions to Acoustic Positional Telemetry: A case study on Atlantic cod around wind turbines in the North Sea
Authors: Knaap Inge Elisabeth Jacoba van der , Slabbekoorn Hans , Winter Hendrik V. , Moens Tom , Reubens Jan 
Published: September 2020

Abstract
Background: An important aspect for the correct interpretation of Acoustic Positional Telemetry (APT) data concerns the effect of individual receiver contributions to animal positioning. The present study evaluated the contribution of each receiver within two APT designs to set-up efficiency and position accuracy of free-ranging Atlantic cod, through sequential single receiver removal. The two APTs were deployed around offshore wind turbines at which 27 individual cod were tagged and released, 50 km offshore, at depths of 20-30 m. Results: Results indicated that removal of an APT receiver, positioned within the movement area of the individual fish, reduced the efficiency and position accuracy of the set-up the most, with a maximum of 34% position loss per fish, and an increase in core area of 97.8%. Removal of a single receiver in general was shown to potentially lead to considerably altered swimming tracks. Additionally, removal of a specific receiver that was deployed within 50 meters from a turbine (i.e. reflective barrier) actually improved fish position accuracy. Conclusions: An explorative, small-scale study like the one presented is recommended, before embarking on any larger scale APT study. Exploration of receiver contributions to set-up performance allow to test the suitability of a receiver set-up for the specific movement patterns and local tracking conditions for a particular target species. Data from receiver arrays from which a receiver was lost should be treated with care, as results revealed that changes in triangulation outcome can lead to considerable shifts in swimming tracks and animal home range estimates.
Analysis of clinical characteristics, laboratory findings and therapy of 134 cases of COVID-19 in Wuhan, China: a retrospective analysis.
Authors: Zhang Rui , Zhang Jie , Chen Jiebing 
Published: September 2020

Abstract
Background：As everyone knows, the pandemic COVID-19 is spreading in the whole world. The number of laboratory-confirmed cases reached 28,637,211 and that of the death cases was 917,404 in the world as of September 13th, 2020. We sought to analyse the clinical characteristics, laboratory findings and therapy of some cases with COVID-19.Methods: In this retrospective study, we extracted the data on 134 patients with laboratory-confirmed COVID-19 in Wuhan Xinzhou District People's Hospital from January 16th to April 24th , 2020. Cases were confirmed by real-time RT-PCR and abnormal radiologic findings. Outcomes were followed up until May 1th , 2020. Results：Co-infection and severe underlying diseases made it easier for a case with COVID-19 to develop to be a severe one or reach an outcome of death. Age above 60 years old, male and symptoms such as fever, cough, chest tightness, headaches and fatigue were related to severe COVID-19 and an outcome of death. In addition, higher temperature, blood leukocyte count, neutrophil count, C-reactive protein level, D-dimer level, alanine aminotransferase activity, aspartate aminotransferase activity,α-hydroxybutyrate dehydrogenase activity, lactate dehydrogenase activity and creatine kinase activity were also related to severe COVID-19 and an outcome of death, and so was lower lymphocyte count. Administration of gamma globulin seemed helpful for reducing the mortality of patients with severe COVID-19, however the P value was greater than 0.05 (P=0.180), which mean under the same condition, studies of larger samples are needed in the future. Conclusion: Multiple factors were related to severe COVID-19 and an outcome of death. Administration of gamma globulin seemed helpful for reducing the mortality of severe cases. More related studies are needed in the future.
Comprehensive assessments of germline deletion structural variants reveal the association between prognostic MUC4 and CEP72 deletions and immune response gene expression in colorectal cancer patients
Authors: Peng-Chan Lin , Chen Hui-O , Lee Chih-Jung , Yeh Yu-Min , Shen Meng-Ru , Chiang Jung-Hsien 
Published: September 2020

Abstract
Background Functional disruptions by large germline genomic structural variants in susceptible genes are known risks for cancer. Few studies have used deletion structural variants (DSVs) to predict cancer risk with neural networks or studied the relationship between DSVs and immune gene expression to stratify prognosis.Methods Whole-genome sequencing (WGS) data was analyzed with the blood samples of 192 cancer and 499 noncancer subjects with or without family cancer history (FCH). Ninety-nine colorectal cancer (CRC) patients had immune response gene expression data. To build the cancer risk predictive model and identify DSVs in familial cancer, we used joint calling tools and attention-weighted model. The survival support vector machine (survival-SVM) was used to select prognostic DSVs. Results We identified 671 DSVs that could predict cancer risk. The area under the curve (AUC) of receiver operating characteristic curve (ROC) of attention-weighted model was 0.71. The 3 most frequent DSV genes observed in cancer patients were identified as ADCY9, AURKAPS1, and RAB3GAP2 (p < 0.05). We identified 65 immune-associated DSV markers for assessing cancer prognosis (P < 0.05). The functional protein of MUC4 DSV gene interacted with MAGE1expresssion, according to the STRING database. The causal inference model showed that deleting the CEP72 DSV gene could affect the recurrence-free survival (RFS) of IFIT1 expression. Conclusions We established an explainable attention-weighted model for cancer risk prediction and used the survival-SVM for prognostic stratification by using DSV and immune gene expression datasets. It can provide the genetic landscape of cancer patients and help predict the clinical outcome.
Predictors and moderators of outcome of psychotherapeutic interventions for mental disorders in young people: Protocol for systematic reviews
Authors: Vousoura Eleni , Gergov Vera , Tulbure Bogdan Tudor , Camilleri Nigel , Saliba Andrea , Garcia-Lopez LuisJoaquin , Podina Ioana R. , Prevendar Tamara , Löffler-Stastka Henriette , Chiarenza Giuseppe Augusto , Debbané Martin , Markovska-Simoska Silvana , Milic Branka , Torres Sandra , Ulberg Randi , Poulsen Stig 
Published: September 2020

Abstract
Background: Adolescence and young adulthood is a risk period for the emergence of mental disorders. There is strong evidence that psychotherapeutic interventions are effective for most mental disorders. However, very little is known about for whom different psychotherapeutic treatment modalities are effective. This large systematic review aims to address this critical gap within the literature on non-specific predictors and moderators of the outcomes of psychotherapeutic interventions among young people with mental disorders. Methods: Pubmed and PsycINFO databases were searched for randomized controlled and quasi-experimental/naturalistic clinical trials. Studies were selected according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Risk of bias of all included studies will be assessed by RoB1 and ROBINS-I risk of bias tools. The quality of predictor and moderator variables will be also assessed. A narrative synthesis will be conducted for all included studies. Discussion: This systematic review will strengthen the evidence base on effective mental health interventions for young people, being the first to explore predictors and moderators of outcome of psychotherapeutic interventions for a wide range of mental disorders in young people. Systematic review registration: PROSPERO (CRD42020166756)
System Dynamics Modeling of Health Workforce Planning to Address Future Challenges of Thailand’s Universal Health Coverage
Authors: Leerapan Borwornsom , Teekasap Pard , Urwannachotima Nipaporn , Jaichuen Wararat , Chiangchaisakultha Kwanpracha , Udomaksorn Khunjira , Meeyai Aronrag Cooper , Noree Thinakorn , Sawaendee Krisada 
Published: September 2020

Abstract
Background: System dynamics modeling can inform policy decisions of healthcare reforms under Thailand’s Universal Health Coverage. We report on this thinking approach to Thailand’s strategic health workforce planning for the next 20 years.Methods: A series of group model building sessions involving 110 participants from multi-sectors of Thailand’s health systems was conducted in 2017 and 2018. Policymakers, healthcare administrators, and practitioners were facilitated to co-create a causal loop diagram representing a shared understanding of why the demands and supplies of the health workforce in Thailand can be mismatched and a stock and flow diagrams for testing the consequences of policy options.Results: Our simulation modeling found hospital utilizations created a vicious cycle of constantly increasing demands for hospital care, and hence a constant shortage of healthcare providers. Moreover, hospital care was not designed for effectively dealing with the future demands of aging populations and prevalent chronic illness. Hence, shifting emphasis to professions that can provide primary care, intermediate care, long-term care, palliative care, and end-of-life care can be more effective.Conclusions: The system dynamics modeling confirmed that shifting the care models to address the changing health demands can be a high-leverage policy of health workforce planning, although very difficult to implement in the short term.
Experimental and numerical modeling for flattening and rapid solidification with crystallization behavior of supersonic ceramic droplets
Authors: Wang Y. , Bai Y , Wu K , Zhou J , Shen M G , Ming Y , Liu Q. , Chong N J , Sun Y W , Hu Y B , Du X J , She Z B 
Published: September 2020

Abstract

Successive impingement of supersonic droplets after refining in plasma jet usually forms a fine-lamellar structured coating with high mechanical properties. However, the comprehensive process (such as flattening, rapid solidification and crystallization) of high-velocity impact of refined droplets is difficult to understand. In this study, an experimental study showed that the content of refinement droplets reached to 90 % and displayed the multi-scale equiaxed grains morphology at extremely rapid solidification rate. Phase-field model revealed a hybrid coalescence growth of oriented attachment and migration of grains boundary under the dynamic temperature gradient. Furthermore, an optimized numerical model that consisted of the Navier-Stokes and energy balance equations coupled with the Cahn-Hilliard and phase-field model for growth orientation of grains was developed to accurately reproduce the comprehensive process of refined supersonic droplets. The size distribution and crystallographic orientation of columnar grains for single or two flattened droplets were in a good agreement with the experimental results. The interface between two-flattened droplets exhibited an epitaxial growth of columnar grains. This optimized model can be an effective method in predicting the flattening and solidification with crystallization behavior of droplets during plasma spraying.
tRNA Ini CAT&nbsp;inhibits proliferation and promotes apoptosis of laryngeal squamous cell carcinoma cell
Authors: Shen Zhisen , Li Jun , Ye Dong , Deng Hongxia , Gu Shanshan , Zhou Chongchang 
Published: September 2020

Abstract
Background: Transfer RNA (tRNA) is a ubiquitous RNA, present in all life forms and considered a housekeeping gene with limited regulatory function. Recent studies have shown that tRNA is dysregulated and involved in the pathogenic process of cancer.Methods: Quantitative reverse transcription-polymerase chain reaction detected the relative expression levels of tRNAIniCAT in paired LSCC tissues and paracancerous tissues and preoperative, postoperative, and healthy human plasma. We analyzed the relationship between its expression level and the clinicopathological features of patients. We also established the receiver operating characteristic curve and predicted its diagnostic value. High expression of tRNAIniCAT was detected in the LSCC cell line. The proliferation, apoptosis, and cycle of cells were analyzed. High expression of tRNAIniCAT was detected in mice with transplanted tumors. The tumor weight and volume in mice were measured. The transplanted tumor was used for pathological analysis and transmission electron microscope observation.Results: tRNAIniCAT was down-regulated in LSCC tissues and plasma. The area under the plasma receiver operating characteristic curve was 0.808. High expression of tRNAIniCAT in the laryngeal carcinoma cell line inhibited cell proliferation and promoted apoptosis. High expression of tRNAIniCAT in transplanted tumor in nude mice inhibited the growth of the transplanted tumor.Conclusion: tRNAIniCAT acts as a tumor Inhibitor in LSCC, inhibits cell proliferation, and promotes apoptosis in vitro and in vivo. tRNAIniCAT may be used as a new biomarker for the early diagnosis of LSCC.
Analyzing the Impact of Covid-19 on the Mothers of Bangladesh: Hearing the Unheard
Authors: Eh Shah Md Azimul , Jahan Fairooz 
Published: September 2020

Abstract
Aim: The purpose of this empirical study is to explore how Covid-19 pandemic has hit the mothers of our society, with particular emphasis on Bangladesh. The study also attempts to make their unheard voice reach both the national and international academic discourse which has so far been an unexcavated area. Subject and Methods: The study adopted qualitative and interpretative methods of social research which include content analysis; perception study of 223 respondents through semi structured questionnaire survey, who were selected using purposive random sampling. The data obtained from perception study was further complemented through phone in interviews. Results: The study has found that pandemic has not affected all the mothers uniformly, rather the intensity of its impact varied depending on factors like occupation of mothers and their husbands and their family pattern. Despite such variation, all the mothers experienced subsequent increase in workload, challenges while availing routine health facilities and higher level of stress, anxiety, depression, and certain behavioral changes. Conclusion: However, the worst affected have been the mothers belonging to the lower socio-economic strata because the pandemic has made them and their husbands’ jobless, leading them towards an uncertain future.