bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three dimensional reconstruction imaging of temporomandibular joint synovial chondromatosis
Authors: Cameron Alice , Richards Charlotte A , Keating Chris , Colbert Serryth D 
Published: December 2020

AbstractAimWe have completed the first documented three dimensional (3D) reconstruction of temporomandibular joint synovial chondromatosis from computed tomography and discuss the application of this technique in diagnosis and treatment of this condition with particular relevance to its surgical management.We describe our case of a 52‐year‐old woman who was admitted to the medical assessment unit with an acute exacerbation of headaches; diagnostic head imaging revealed left TMJ calcification most suggestive of synovial osteochondromatosis.Methods and materialsMaterialise Mimics inPrint 3.0 is an advanced tool that allows for the import of medical image data from either CT or magnetic resonance imaging (MRI) as DICOM files. The data is analysed by the user and then an image is segmented in an accessible environment resulting in an accurate 3D anatomical model that can be 3D printed or visualised in either augmented reality or virtual reality (AR/VR).ResultsWe demonstrate our novel 3D reconstruction of this florid appearance of TMJ SC from an incidental finding on CT.ConclusionThis is an unusual presentation of a rare condition; despite the florid appearance on imaging, the patient’s function was entirely normal. TMJ SC is a benign condition but can require surgical management. We present our novel 3D imaging technique for diagnosis and to aid surgical management.
The CC‐NB‐LRR OsRLR1 mediates rice disease resistance through interaction with OsWRKY19
Authors: Du Dan , Zhang Changwei , Xing Yadi , Lu Xin , Cai Linjun , Yun Han , Zhang Qiuli , Zhang Yingying , Chen Xinlong , Liu Mingming , Sang Xianchun , Ling Yinghua , Yang Zhenglin , Li Yunfeng , Lefebvre Benoit , He Guanghua 
Published: December 2020

SummaryNucleotide‐binding site–leucine‐rich repeat (NB‐LRR) resistance proteins are critical for plant resistance to pathogens; however, their mechanism of activation and signal transduction is still not well understood. We identified a mutation in an as yet uncharacterized rice coiled‐coil (CC)‐NB‐LRR, Oryza sativa RPM1‐like resistance gene 1 (OsRLR1), which leads to hypersensitive response (HR)‐like lesions on the leaf blade and broad‐range resistance to the fungal pathogen Pyricularia oryzae (syn. Magnaporthe oryzae) and the bacterial pathogen Xanthomonas oryzae pv. oryzae, together with strong growth reduction. Consistently, OsRLR1‐overexpression lines showed enhanced resistance to both pathogens. Moreover, we found that OsRLR1 mediates the defence response through direct interaction in the nucleus with the transcription factor OsWRKY19. Down‐regulation of OsWRKY19 in the rlr1 mutant compromised the HR‐like phenotype and resistance response, and largely restored plant growth. OsWRKY19 binds to the promoter of OsPR10 to activate the defence response. Taken together, our data highlight the role of a new residue involved in the NB‐LRR activation mechanism, allowing identification of a new NB‐LRR downstream signalling pathway.
Lysine crotonylation of DgTIL1 at K72 modulates cold tolerance by enhancing DgnsLTP stability in chrysanthemum
Authors: Huang Qiuxiang , Liao Xiaoqin , Yang Xiaohan , Luo Yunchen , Lin Ping , Zeng Qinhan , Bai Huiru , Jiang Beibei , Pan Yuanzhi , Zhang Fan , Zhang Lei , Jia Yin , Liu Qinglin 
Published: December 2020

SummaryLysine crotonylation of proteins is a recently identified post‐translational modification (PTM) in plants. However, the function of lysine‐crotonylated proteins in response to abiotic stress in plants has not been reported. In this study, we identified a temperature‐induced lipocalin‐1‐like gene (DgTIL1) from chrysanthemum and showed that it was notably induced in response to cold stress. Overexpression of DgTIL1 enhanced cold tolerance in transgenic chrysanthemum. Ubiquitin membrane yeast two‐hybrid (MYTH) system and bimolecular fluorescence complementation (BIFC) assays showed that DgTIL1 interacts with a nonspecific lipid transfer protein (DgnsLTP), which can promote peroxidase (POD) gene expression and POD activity to reduce the accumulation of reactive oxygen species (ROS) and improve resistance to cold stress in DgnsLTP transgenic chrysanthemum. In addition, we found that DgTIL1 was lysine crotonylated at K72 in response to low temperature in chrysanthemum. Moreover, lysine crotonylation of DgTIL1 prevented DgnsLTP protein degradation in tobacco and chrysanthemum. Inhibition of DgnsLTP degradation by lysine crotonylation of DgTIL1 further enhanced POD expression and POD activity, reduced the accumulation of ROS under cold stress in DgTIL1 transgenic chrysanthemum, thus promoting the cold resistance of chrysanthemum.
Wheat FRIZZYPANICLE activates VERNALIZATION1‐A and HOMEOBOX4‐A to regulate spike development in wheat
Authors: Li Yongpeng , Li Long , Zhao Meicheng , Guo Lin , Guo Xinxin , Zhao Dan , Batool Aamana , Dong Baodi , Xu Hongxing , Cui Sujuan , Zhang Aimin , Fu Xiangdong , Li Junming , Jing Ruilian , Liu Xigang 
Published: December 2020

SummaryKernel number per spike determined by the spike or inflorescence development is one important agricultural trait for wheat yield that is critical for global food security. While a few important genes for wheat spike development were identified, the genetic regulatory mechanism underlying supernumerary spikelets (SSs) is still unclear. Here, we cloned the wheat FRIZZY PANICLE (WFZP) gene from one local wheat cultivar. WFZP is specifically expressed at the sites where the spikelet meristem and floral meristem are initiated, which differs from the expression patterns of its homologs FZP/BD1 in rice and maize, indicative of its functional divergence during species differentiation. Moreover, WFZP directly activates VERNALIZATION1 (VRN1) and wheat HOMEOBOX4 (TaHOX4) to regulate the initiation and development of spikelet. The haplotypes analysis showed that the favourable alleles of WFZP associated with spikelet number per spike (SNS) were preferentially selected during breeding. Our findings provide insights into the molecular and genetic mechanisms underlying wheat spike development and characterize the WFZP as elite resource for wheat molecular breeding with enhanced crop yield.
QTL analysis of deep‐sowing tolerance during seed germination in the maize IBM Syn4 RIL population
Authors: Han Qinghui , Shen Yao , Lv Lv , Lee Michael , Lübberstedt Thomas , Zhao Guangwu 
Published: December 2020

AbstractIn arid or semi‐arid regions, deep‐sowing is an effective treasure to ensure seeds absorbing water from deep soil layer at present. However, the existing maize varieties have poor tolerance to deep‐sowing, which is attributed to that few genes are explored and utilised. In this study, 243 IBM Syn4 recombinant inbred lines (RIL) constructed with B73 and Mo17 as parents and 1,339 DNA markers evenly distributed in 10 chromosomes, were used for QTL analysis of deep‐sowing tolerance during seed germination. There were significant differences in germination‐related traits between the parental lines at 12.5 cm sowing depth. Among them, 7, 7, 5, 10 and 2 QTLs for emergence rate, seedling length, plumule length, mesocotyl length and coleoptile length were detected, respectively. These QTLs explained 2.75% to 10.49% of the phenotypic variance with LOD scores ranging from 2.50 to 8.27. In addition, 12 overlapping QTLs formed five QTL clusters on chromosomes 3, 5, 7 and 9. This study provides a basis for molecular marker‐assisted breeding and functional study in deep‐sowing germination of maize.
Uptake of next‐generation sequencing in children with end‐stage renal disease secondary to focal segmental glomerulosclerosis and parental decision for kidney transplantation—Experience from a low resource setting: A Retrospective Cohort Study
Authors: Sinha Rajiv , Sarkar Subhankar , Mandal Kausik , Tse Yincent 
Published: December 2020

AbstractBackgroundTransplantation is the optimal modality for children with ESRD. High risk of disease recurrence and graft loss with FSGS, and its financial implications, may result in families refusing transplantation. Deceased donation is often preferred for FSGS, but access is limited in many low‐ and middle‐income group countries (LMIC; per capita gross national income between $1026 and $3995). As FSGS secondary to an underlying genetic etiology has low recurrence risk, we hypothesized that in LMIC such as India, families with children in ESRD secondary to FSGS with proven pathogenic mutation are more likely to consent for transplantation than those with unknown etiology.MethodsProspective cross‐sectional study with retrospective chart review was undertaken (March 2011 and February 2019) to identify children with ESRD from FSGS. The objective was to ascertain NGS uptake and findings, parental decision for transplantation, and transplant outcome.Results28 children with FSGS started transplant workup, and 15 (54%) families agreed for NGS testing. Pathogenic mutation (NPHS1 x 2, WT1 x 2, COL4A3 x 2, CD2AP, CRB2, COL4A5, INF 2, ACTN4, NPHP4: 1 each) was identified in 12 (80%). 92% (11/12) agreed to proceed with transplantation in contrast to 13% (2/16) who either did not undergo NGS testing or had no pathogenic mutation identified (P = .001). No disease recurrences were noted in those with a known pathogenic mutation.ConclusionIn LMIC, NGS results are useful in transplant discussions with families for children with ESRD secondary to FSGS.
Young Hispanic fathers during COVID‐19: Balancing parenthood, finding strength, and maintaining hope
Authors: Recto Pamela , Lesser Janna 
Published: December 2020

AbstractObjectivesThis study examined how the effects of the COVID‐19 crisis has impacted young Hispanic fathers.Design/SampleUsing qualitative description, in‐depth interviews were conducted among Hispanic fathers between the ages of 18 and 24 years, from community‐based fatherhood program. The interviews of seven young Hispanic fathers were analyzed using qualitative content analysis.ResultsThe participants’ initial and ongoing fears about COVID‐19 resulted from not having adequate information about the severity of the disease and how easily one can contract and transmit it to others. They also expressed concerns about the threat of the pandemic on their family's well‐being, finances, and employment status. Providing for their families was their main priority, with some continuing to work, despite the risks of becoming infected with COVID‐19. Despite their many hardships, fathers found strength in their families and remained hopeful in overcoming the challenges during the pandemic.ConclusionsThe pandemic has presented difficulties and loss for many. Nurses in the community are well positioned to serve young Hispanic fathers to ensure their needs are met. A family‐centered approach is ideal for young fathers to provide them equal opportunity to be actively involved in promoting health for themselves and their families during the pandemic.
Implementing electronic health record‐based National Diabetes Prevention Program referrals in a rural county
Authors: Olsen Jeanette , Peterson Samantha , Stevens Alisyn 
Published: December 2020

AbstractBackgroundSince 2016, public health professionals in a rural, Midwest county have collaborated with competing health care organizations to establish electronic health record (EHR)‐based systems for identifying patients with prediabetes and referring them to National Diabetes Prevention Program (NDPP) classes. The purpose of this program evaluation was to identify barriers and best practices learned from implementation in two rural health care organizations. Methods. A purposively selected program coordinator from each organization and a primary care provider and information technology administrator from one of the organizations provided perspectives on initiating the referral process. Data were collected using open‐ended questions in electronic survey format. Content analysis was used to identify themes. Results. Two barrier themes were identified: Timely, clear communication is challenging; and More education is needed for both patients and providers. Best practice themes included Start small with one provider champion; Create a referral process that doesn't exceed resources; and Providers should be actively involved in referral discussions and decisions. Conclusion. Retrospective queries are a feasible way to initially establish EHR‐based referral programs in rural areas. PHNs can facilitate this system change among competing health care organizations by leading partnerships, identifying common goals and timelines, and providing space for sharing challenges and best practices.
Interactions between Borrelia burgdorferi and its hosts across the enzootic cycle
Authors: Helble Jennifer D. , McCarthy Julie E. , Hu Linden T. 
Published: December 2020

AbstractThe bacterial pathogen Borrelia burgdorferi is the causative agent of Lyme disease and is transmitted to humans through an Ixodes tick vector. B. burgdorferi is able to survive in both mammalian and tick hosts through careful modulation of its gene expression. This allows B. burgdorferi to adapt to the environmental and nutritional changes that occur when it is transmitted between the two hosts. Distinct interactions between the spirochete and its host occur at every step of the enzootic cycle and dictate the ability of the spirochete to survive until the next stage of the cycle. Studying the interface between B. burgdorferi, the Ixodes tick vector and the natural mammalian reservoirs has been made significantly more feasible through the complete genome sequences of the organisms and the advent of high throughput screening technologies. Ultimately, a thorough investigation of the interplay between the two domains (and two phyla within one domain) is necessary in order to completely understand how the pathogen is transmitted.
Respiratory quotient and the stoichiometric approach to investigating metabolic energy substrate use in aquatic ectotherms
Authors: Wang Shuangyao , Carter Chris G. , Fitzgibbon Quinn P. , Smith Gregory G. 
Published: December 2020

AbstractThe respiratory quotient (RQ) has been used extensively as an index to evaluate metabolic energy expenditure in terrestrial animals including humans. In contrast, RQ use in understanding physiology and nutrition of aquatic ectotherms has been restricted due to technical challenges in measuring total CO2in water. With technical advances in measuring total CO2in water, RQ in aquatic ectotherms can be accurately determined and is potentially available as a valuable method. Here, we provide a comprehensive review of studies on RQ and metabolic energy substrate use in aquatic ectotherms. Metabolic energy substrate use is evaluated by a reliable stoichiometric bioenergetic approach, based on measuring RQ and nitrogen quotient (NQ) simultaneously. Stoichiometry provides a non‐destructive and unequivocal way to quantify the instantaneous oxidation of each major energy substrate (protein, lipid or carbohydrate). This review aims to refine knowledge about bioenergetics of aquatic ectotherms under different conditions including nutritional aspects of sustainable aquaculture. Notably, stoichiometry provides a promising approach to optimize feeds and feeding regimes to realize sustainable aquaculture under differing conditions and with differing feed ingredients. It also provides an approach to consider climate change impacts and physiological adaptation mechanisms for survival and development in farmed environments and natural ecosystems. Stoichiometric bioenergetics knowledge in aquatic ectotherms has relevance to commercial impacts in the face of overfishing and food security, and ecological significance in the face of environmental change scenarios. We suggest expanding the use of stoichiometry in future bioenergetic research in emerging aquaculture species.
Timed artificial insemination in crossbred mares: Reproductive efficiency and costs
Authors: Macan Rodrigo Chaves , Camargo Carlos Eduardo , Zielinski Bruna Lampe , Cardoso Nathália Gonçalves Hesketh , de Lara Natália Santana Siqueira , Bergstein‐Galan Tácia Gomes , Weiss Romildo Romualdo , Kozicki Luiz Ernandes 
Published: December 2020

AbstractTimed artificial insemination (TAI) has boosted the use of conventional artificial insemination (CAI) by employing hormonal protocols to synchronize oestrus and ovulation. This study aimed to evaluate the efficiency of a hormonal protocol for TAI in mares, based on a combination of progesterone releasing intravaginal device (PRID), prostaglandin (PGF2α) and human chorionic gonadotropin (hCG); and compare financial costs between CAI and TAI. Twenty‐one mares were divided into two groups: CAI group (CAIG; n = 6 mares; 17 oestrous cycles) and TAI group (TAIG; n = 15 mares; 15 oestrous cycles). The CAIG was subjected to CAI, involving follicular dynamics and uterine oedema monitoring with ultrasound examinations (US), and administration of hCG (1,600 IU) when the dominant follicle (DF) diameter's ≥35 mm + uterine oedema + cervix opening. The AI was performed with fresh semen (500 × 106 cells), and embryo was recovered on day 8 (D8) after ovulation. In TAI, mares received 1.9 g PRID on D0. On D10, PRID was removed and 6.71 mg dinoprost tromethamine was administered. Ovulation was induced on D14 (1,600 IU of hCG) regardless of the DF diameter's, and AI was performed with fresh semen (500 × 106 cells). On D30 after AI, pregnancy was confirmed by US. The pregnancy rate was 80.0% in TAIG and 82.3% in CAIG (p > .05). The TAI protocol resulted in 65% reduction in professional transport costs, and 40% reduction in material costs. The TAI was as efficient as CAI, provided reduction in costs and handlings, and is recommended in mares.
Optimizing injection time of GFP plasmid into sheep zygote
Authors: Ebrahimi Mohammadreza , Mara Laura , Chessa Bernardo , Chessa Fabrizio , Parham Abbas , Dattena Maria 
Published: December 2020

AbstractMicroinjection of exogenous DNA into the cytoplasm of matured oocytes or zygotes is a promising technique to generate transgenic animals. However, the data about the microinjection time and procedure in sheep are limited and have not treated in detail. To obtain more in‐depth information, the Sarda sheep oocytes from abattoir‐derived ovaries were subjected to IVM and IVF. Then, the GFP plasmid as a reporter gene was injected into the cytoplasm of MII oocytes (n: 95) and zygotes at different post‐insemination intervals (6–8 hpi, n: 120; 8–10 hpi, n: 122; 10–12 hpi, n: 110 and 12–14 hpi, n: 96). There were no significant differences in the cleavage rates between the groups. However, blastocyst rate of injected zygotes at all‐time intervals was significantly lower than injected MII oocytes and control group (p < 0.05). Interestingly, the proportion of GFP‐positive embryos was higher at 8–10 hpi compared with other injected groups (4 % versus 0 %, p  < 0.01). Among these, the proportion of mosaic embryos was high and two of those embryos developed to the blastocyst stage. In conclusion, we settled on the cytoplasmic microinjection of GFP plasmid at 8–10 hpi as an optimized time point for the production of transgenic sheep and subsequent experiments.
Why meta‐research matters to regulation and governance scholarship: An illustrative evidence synthesis of responsive regulation research
Authors: van der Heijden Jeroen 
Published: December 2020

AbstractThis article is an introduction to meta‐research, a systematic and replicable process of synthesizing research findings across a body of original research. After introducing the reader to the core of meta‐research methodology, meta‐research logic and tools are applied to present an evidence synthesis of empirical research on responsive regulation. The article concludes with a meta‐research agenda for regulation and governance scholarship, and five key lessons from the empirical responsive regulation literature.
Suicidal and violent ideation in acute psychiatric inpatients: prevalence, co‐occurrence, and associated characteristics
Authors: Furnes Desiré , Gjestad Rolf , Rypdal Knut , Mehlum Lars , Hart Stephen , Oedegaard Ketil J. , Mellesdal Liv 
Published: December 2020

AbstractObjectiveWe studied the point prevalence of suicidal and violent ideation, as well as their co‐occurrence and associated characteristics in inpatients with mental health disorders.MethodsData on suicidal and violent ideation, and sociodemographic and clinical information, were gathered from 1,737 patients when admitted to the acute psychiatric ward.ResultsThe point prevalence was 51.9% for suicidal ideation and 19.8% for violent ideation. The point prevalence of co‐occurring suicidal and violent ideation was 12.3%, which was significantly greater than expected by chance. Logistic regression analyses indicated that both suicidal and violent ideation were associated with young age and the absence of diagnoses of psychotic disorders; in addition, suicidal ideation was associated with female gender, violent ideation, and diagnoses of mood‐ neurotic and personality disorders, whereas violent ideation was associated with male gender, suicidal ideation, and diagnoses of mood and neurotic disorders.ConclusionsOverall, the findings highlight the need for further research on suicidal and violent ideation in people with mental health problems including, but not limited to, their association with adverse behavioral outcomes, as well as the need to routinely assess both suicidal and violent ideation in clinical practice.
Adorno's negative psychology
Authors: Strosberg Benjamin B. 
Published: December 2020

AbstractFollowing the Second World War, Adorno et al.’s The Authoritarian Personality (1950/2019) became the most influential engagement between Frankfurt School critical theorists and the field of psychology. The recent publication of Adorno's retracted “Remarks on The Authoritarian Personality” (2019) provides a timely impetus to reexamine and challenge certain long‐held ideas about Adorno's contribution to psychology—for instance that The Authoritarian Personality reduces complex social phenomena to individual motivation and personality. Adorno's Remarks provide an alternative approach to the usual reading of The Authoritarian Personality, where the psychology of authoritarianism points to a contradiction at the very core of the concept of psychology, aligning the study with Adorno's more critical and dialectical works. In this article, I challenge how Adorno and The Authoritarian Personality are commonly taken up in critical psychology and introduce “negative psychology” as Adorno's central contribution to the field modeled in his reading of psychoanalysis and his theory of negative dialectics. Reframed in this way, Adorno can continue to serve as a crucial site of critical engagement for psychologists.
Who Are “the Immigrants”? Beliefs About Immigrant Populations and Anti‐Immigration Attitudes in the United States and Britain
Authors: Zhirkov Kirill 
Published: December 2020

ObjectivesThere is important variation in beliefs about immigration within the publics of immigrant‐receiving societies but empirical social research has largely overlooked it. The “imagined immigration” concept aims to bring these beliefs back in as an important component of public attitudes toward immigrants. It also offers a new perspective on the political conflict around immigration that persists despite the consensus on desired qualities of potential immigrants among citizens in industrial democracies.MethodsI review the imagined immigration concept and present new empirical evidence in its favor using original survey studies in the United States and Britain.ResultsI describe respondents’ beliefs about immigrants, demonstrate their association with partisanship, and confirm that these beliefs are significantly related to perceptions of immigration as harmful—even when controlled for partisanship, ideology, and ethnocentrism.ConclusionI corroborate the foundations of the imagined immigration concept and discuss its promise and limitations.