bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of SARS‐CoV‐2 mutations in Mexico, Belize, and isolated regions of Guatemala and its implication in the diagnosis
Authors: Hernández‐Huerta María Teresa , Pérez‐Campos Mayoral Laura , Romero Díaz Carlos , Martínez Cruz Margarito , Mayoral‐Andrade Gabriel , Sánchez Navarro Luis Manuel , Pina‐Canseco María Del Socorro , Cruz Parada Eli , Martínez Cruz Ruth , Pérez‐Campos Mayoral Eduardo , Pérez Santiago Alma Dolores , Vásquez Martínez Gabriela , Pérez‐Campos Eduardo , Matias‐Cervantes Carlos Alberto 
Published: October 2020

AbstractThe genomic sequences of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) worldwide are publicly available and are derived from studies due to the increase in the number of cases. The importance of study of mutations is related to the possible virulence and diagnosis of SARS‐CoV‐2. To identify circulating mutations present in SARS‐CoV‐2 genomic sequences in Mexico, Belize, and Guatemala to find out if the same strain spread to the south, and analyze the specificity of the primers used for diagnosis in these samples. Twenty three complete SARS‐CoV‐2 genomic sequences, available in the GISAID database from May 8 to September 11, 2020 were analyzed and aligned versus the genomic sequence reported in Wuhan, China (NC_045512.2), using Clustal Omega. Open reading frames were translated using the ExPASy Translate Tool and UCSF Chimera (v.1.12) for amino acid substitutions analysis. Finally, the sequences were aligned versus primers used in the diagnosis of COVID‐19. One hundred and eighty seven distinct variants were identified, of which 102 are missense, 66 synonymous and 19 noncoding. P4715L and P5828L substitutions in replicase polyprotein were found, as well as D614G in spike protein and L84S in ORF8 in Mexico, Belize, and Guatemala. The primers design by CDC of United States showed a positive E value. The genomic sequences of SARS‐CoV‐2 in Mexico, Belize, and Guatemala present similar mutations related to a virulent strain of greater infectivity, which could mean a greater capacity for inclusion in the host genome and be related to an increased spread of the virus in these countries, furthermore, its diagnosis would be affected.
Rapid ultrasound‐assisted microextraction of atorvastatin in the sample of blood plasma by nickel metal organic modified with alumina nanoparticles
Authors: Bahrani Sonia , Ghaedi Mehrorang , Asfaram Arash , Mansoorkhani Mohammad Javad Khoshnood , Javadian Hamedreza 
Published: October 2020

AbstractIn the present work, nickel‐1,4‐benzenedioxyacetic acid was synthesized as a rod‐like metal organic material and then modified with alumina nanoparticles to synthesize nickel metal organic modified‐Al2O3 nanoparticles. The material was found as an efficient sorbent for the enrichment of atorvastatin in human blood plasma. After the extraction of the sample of plasma by ultrasound‐assisted dispersive solid phase extraction, high performance liquid chromatography‐ultraviolet was used to determine the quantitatively pre‐concentrated interest analyte. The conditions for optimum extraction were achieved by the optimization of the volume of eluent, dosage of the sorbent, and time of sonication. Solution pH of 7.0, 250 μL of ethanol, 45 mg of the sorbent, and 10 min of sonication time were the conditions for extracting the atorvastatin maximum recovery of higher than 97.0%. By using desirability function for the optimization of the process, the present method showed a response that was linear ranging from 0.2 to 800 ng/mL with regression coefficient of 0.999 in the plasma of human blood with a satisfactory detection limit of 0.05 ng/mL, while the precision of interday for the current method was found to be <5%. It can be concluded that dispersive solid phase extraction method is effective for the extraction of atorvastatin from human plasma samples (97.4‐102%) due to its easy operation, simplicity, repeatability, and reliability.
Mechanistic insights into biomimetic CO2 hydration activity of titania nanoclusters
Authors: Verma Manju , Deshpande Parag A. 
Published: October 2020

AbstractAnatase TiO2, a widely used semiconductor metal oxide support, was tested for biomimetic carbonic anhydrase activity catalyzing the reversible hydration of CO2 to bicarbonate ions. Owing to superior photocatalytic response of nanoclusters of TiO2 and its apparent role in catalyzing CO2 hydration, (TiO2)15 nanocluster was tested for the reaction using density functional theory calculations. Free energy landscape of the reaction was developed following the biological mechanism consisting of H2O adsorption, deprotonation, CO2 complexation, CO2 bending, intramolecular proton transfer, and bicarbonate ion displacement. The identity of the intermediates of the mechanistic cycle was further confirmed by computational nuclear magnetic resonance and infrared spectroscopic studies. A close correspondence of the computational and reported experimental spectroscopic analyses confirmed the identity of the intermediates and the presence of biomimesis over the TiO2 cluster.
Photoluminescence and Energy Transfer Study of Sr3Y0.96(BO3)3:0.02Ce3+0.02Mn2+ Phosphor for WLED Application
Authors: Hargunani Sanjay P. , Sonekar Rajkumar P. , Dhoble Sanjay J. , Omanwar Shreeniwas K. 
Published: October 2020

AbstractSr3Y(BO3)3;Sr3Y0.98(BO3)3:0.02Ce3+;Sr3Y0.98(BO3)3:0.02Mn2+ Sr3Y0.96(BO3):0.02Ce3+0.02Mn2+phosphors are synthesized by solution combustion method at 650 °C in muffle furnace. Synthesized phosphors are annealed at 950 °C for 3 h under weak reducing atmosphere provided by activated charcoal/carbon to achieve desire oxidation state of dopants. Photoluminescence study shows that Sr3Y0.98(BO3)3:0.02Mn2+ phosphors converts blue light (462 nm) into red (614 nm) due to 4T1(4G) to 6A1(6s) transition of Mn2+ in host crystal. Sr3Y0.98(BO3)3:0.02Ce3+ converts UV radiation (261 nm: 2f5/2→5d1 of Ce3+) into blue light of wavelength 486 nm which is attributed to the inter‐configuration 5d1→4f1 transition of Ce3+. In Sr3Y0.96(BO3)3:0.02Ce3+ 0.02Mn2+ phosphors resonant energy transfer occurs from Ce3+ to Mn2+ in host matrix, resulting into intense red emission (614 nm) from UV light (263 nm). Using photoluminescence intensity comparison, the study confirms the resonant‐ET from sensitizer Ce3+ to activator Mn2+ in the crystal. So the report it as UV‐activated red emitting phosphor for UV‐PC‐WLED.
Superhydrophobic Coating Using TiO2 NPs/PMHS Composite for Self‐Cleaning Application
Authors: Sutar Rajaram S. , Manadeshi Shriram D. , Latthe Sanjay S. , Kulal S. R. , Salunkhe G. D. , Rangar K. K. , Lavate R. A. , Raut S. B. , Sapkal A.C. , Bhosale Appasaheb K. , Sadasivuni Kishor Kumar , Liu Shanhu , Xing Ruimin 
Published: October 2020

AbstractIn the present research work, spray deposition technique is adopted for the fabrication of superhydrophobic coating on glass slide using TiO2 nanoparticles and polymethylhydrosyloxane (PMHS) composite. The prepared superhydrophobic coating revealed hierarchical surface morphology due to different micro and nanoscaled grains of TiO2 NPs/PMHS composite. The water drops hardly stay on the superhydrophobic coating and roll off the surface at sliding angle of 6° due to high water contact angle of 163 ± 2°. As a result, the prepared superhydrophobic coating revealed excellent self‐cleaning performance. To evaluate the mechanical durability the prepared superhydrophobic coating, the coating surface is exposed to water jet, water drop impact, adhesive tape peeling, and sandpaper abrasion tests. This coating approach can be applied to the substrates of any size and shape.
Combustion Synthesis and Photoluminescence Studies of Blue‐Emitting CaAl12O19:Ce3+ Lamp Phosphors
Authors: Panse V. R. , Choubey S. R. , Pattanaik Amitansu , Dhoble S. J. 
Published: October 2020

AbstractThe spectroscopic properties of Ce3+ with a favorable response and the ability to integrate the Ce3+ ion into diverse host inorganic material, trivalent cerium‐activated material is activated with cerium ion cover transformed interest for most of the applications. These materials recombine with high light capitulate, constructive emission wavelength, and faster fluorescence decay with stability of temperature, which make them attractive for use in detectors for elevated energy physics and also medical imaging. Therefore, with the support of outstanding luminescence properties, inorganic materials activated with Ce3+ ions are applied in lightings industries, detectors with display systems for ionizing radiation. Here synthesis and photoluminescence (PL) investigation of CaAl12O19:Ce3+ carried out using combustion synthesis method. The structural and morphological studies and confirmation of phase and purity are done using XRD and SEM. PL spectra of Ce3+ due to the 4f–5d transition of Ce3+ ions peaking at 331 nm. The photoluminescence emission spectra of CaAl12O19:Ce3+ phosphor exhibit blue emission band centered at 440 nm. Usually, configuration of Ce3+ ion in ground state is divide into two levels, viz., 2F5/2 and 2F7/2 whereas the 5d1 excited configuration is divide by the crystal playing field ranging from 2 to 5 components.
Photocatalytic Environmental Remediation of Cassiterite–Titania Nanocomposite
Authors: Mane Chandrakant B. , Pawar Ramkrushna P. , Patil Rajendra P. , Patil Sarjerao B. 
Published: October 2020

AbstractNanocrystalline cassiterite (SnO2), titania (TiO2), and cassiterite–titania (SnO2–TiO2) are synthesized by microwave method. X‐ray diffraction study reveals that cassiterite properly supported on the surface of titania. Nano sized cassiterite, titania, and cassiterite–titania nanocomposite are confirmed by transmission electron microscopy technique. The particle size of the SnO2, TiO2, and their nanocomposite is in the range of 12–18 nm. The enhanced photocatalytic activity is observed in the SnO2–TiO2 composite as compared to SnO2 and TiO2.
Effect of Sintering Temperature on Structural, Morphological, and Magnetic Properties of Nickel Ferrite Prepared via a Polyol Method
Authors: Patil Rajendra P. , Waghmare Mahendra B. , Kalantre Vilas M. , Kadam Mohan R. , Chikalkar Milind G. , Delekar Sagar D. 
Published: October 2020

AbstractThe effects of sintering temperature on the structural and magnetic properties of polycrystalline nickel ferrite synthesized by the novel polyol method are investigated. The effects of heat treatment temperature on crystalline phases formation, microstructure, and magnetic properties of Ni ferrite are investigated by x‐ray diffraction (XRD), thermogravimetric, and differential thermal analysis, scanning electron microscopy, transmission electron microscopy, Fourier‐transform infrared spectroscopy (FT‐IR), and B‐H loop tracer technique. The XRD plot of the powder samples sintered at different temperature shows increase in the peak intensity values with respect to temperature of sintering thereby showing an increasing crystallinity of the samples with an increasing sintering temperature. FT‐IR spectra for sample sintered at 773 K shows two sharp bands on octahedral and tetrahedral sites. Scanning electron micrographs show spherical and uniform grains which is observed on sintering temperature 773 K. Particle size of nickel ferrite is in the range of 11–15 nm, and depends on the sintering temperature and sintering time. The saturation magnetization increased from 23.45 to 45.61 emu g−1 and coercivity increases from 327.27 to 1097.03 Oe with an increase in particle size.
Chemical molecular‐based approach to overcome multidrug resistance in cancer by targeting P‐glycoprotein (P‐gp)
Authors: Zhang Hang , Xu Haiwei , Ashby Charles R. , Assaraf Yehuda G. , Chen Zhe‐Sheng , Liu Hong‐Min 
Published: October 2020

AbstractMultidrug resistance (MDR) remains one of the major impediments for efficacious cancer chemotherapy. Increased efflux of multiple chemotherapeutic drugs by transmembrane ATP‐binding cassette (ABC) transporter superfamily is considered one of the primary causes for cancer MDR, in which the role of P‐glycoprotein (P‐gp/ABCB1) has been most well‐established. The clinical co‐administration of P‐gp drug efflux inhibitors, in combination with anticancer drugs which are P‐gp transport substrates, was considered to be a treatment modality to surmount MDR in anticancer therapy by blocking P‐gp‐mediated multidrug efflux. Extensive attempts have been carried out to screen for sets of nontoxic, selective, and efficacious P‐gp efflux inhibitors. In this review, we highlight the recent achievements in drug design, characterization, structure–activity relationship (SAR) studies, and mechanisms of action of the newly synthetic, potent small molecules P‐gp inhibitors in the past 5 years. The development of P‐gp inhibitors will increase our knowledge of the mechanisms and functions of P‐gp‐mediated drug efflux which will benefit drug discovery and clinical cancer therapeutics where P‐gp transporter overexpression has been implicated in MDR.
Use of sliced or minced peripheral nerve segments for nerve regeneration through a biodegradable nerve conduit: A preliminary study in the rat
Authors: Nagumo Yoshiaki , Isogai Noritaka , Kusuhara Hirohisa , Asamura Shinichi , Onodera Yuta , Teramura Takeshi , Sueyoshi Yu 
Published: October 2020

AbstractBackgroundUsing the rat sciatic nerve model, the difference in outcome using a nerve segment either sliced open or minced with a blade incorporated into a nerve conduit were compared and the relative effects upon the rate and completeness of the nerve regeneration was determined.Materials and MethodsA 10‐mm gap was created in the rat sciatic nerve and bridged with a biodegradable nerve conduit. Segments of the resected nerve (2‐mm lengths) were prepared by either slicing the nerve with one longitudinal cut or by scalpel mincing of the nerve tissue, with insertion of the prepared nerve segment into the center of the conduit. Flow cytometry and Western blotting of these preparations were performed to measure viable cells and to examine the expression of Erk1/2 for neural regeneration potential with both treatments. in vivo nerve regeneration was evaluated at 2, 4, 8, and 20 weeks, using immunohistochemistry, transmission electron microscopy, muscle wet weight, and nerve conduction velocity determination.ResultsThe sliced nerve group showed significantly greater Schwann cell migration with the subsequent axonal elongation at 4 weeks after implantation, in comparison to the minced nerve group and controls (unaltered conduit grafts). By 20 weeks anterior tibial muscle weight and nerve conduction velocity were also greater in the sliced nerve group in comparison to the other groups (p < .05).ConclusionThese findings suggest that insertion of a sliced section of nerve into a biodegradable nerve conduit can shorten the time for and improve the quality of nerve regeneration.
Effect of acquisition rates on off‐task behavior of kindergarten students while learning sight words
Authors: Burns Matthew K. , Aguilar Lisa N. , Warmbold‐Brann Kristy , Preast June L. , Taylor Crystal N. 
Published: October 2020

AbstractAssessing a student's acquisition rates (ARs) is a reliable way to determine how many new words should be taught in one lesson without reducing retention. Exceeding a student's AR can result in frustration and problem behaviors. The purpose of this study was to examine the effect of AR on the off‐task behavior of kindergarten students while participating in a commonly used sight‐word instruction video. Participants included 39 kindergarten students whose ARs were assessed before showing the sight‐word video. Behavior was measured as on‐ and off‐task using momentary time‐sampling with 10‐s intervals. Results indicated that students' time off task increased after exceeding their ARs, with a noticeable immediate increase. The implications and limitations of these results are discussed.
Rhodiola crenulata protects against Alzheimer's disease in rats: A brain lipidomics study by Fourier‐transform ion cyclotron resonance mass spectrometry coupled with high‐performance reversed‐phase liquid chromatography and hydrophilic interaction liquid chromatography
Authors: Sun Wei , Liu Chun , Wang Yanan , Zhou Xing , Sui Wenwen , Zhang Yu , Zhang Qingyu , Han Jing , Li Xintong , Han Fei 
Published: October 2020

RationaleAlzheimer's disease (AD) is a chronic, severe, progressive neurodegenerative disorder associated with cognitive and memory impairment that ultimately causes death. Most approved drugs can only alleviate some of the symptoms of AD, but no interventions have been found that reverse the underlying disease mechanisms. Rhodiola crenulata extract (RCE) has been reported to alleviate AD symptoms in rats. However, its underlying mechanism of action is still unclear.MethodsA brain lipidomics study was conducted to investigate the protective effects of RCE against AD in rats to identify potential biomarkers of AD using Fourier‐transform ion cyclotron resonance mass spectrometry (FT‐ICR MS) coupled with high‐performance reversed‐phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC). Differences in lipid metabolism profiles were evaluated using multivariate statistical analysis. Finally, the possible mechanism of action of RCE on AD was investigated by analysing metabolic pathways.ResultsThe RPLCHILIC/FT‐ICR MS results showed 20 lipid components with significant differences between the control and model groups. After administration of RCE, the levels of 10 lipids in AD rats tended to shift toward reference levels. The pathway analysis revealed that the protective effect of RCE against AD might be related to regulation of glycerophospholipid metabolism.ConclusionsThis study provides a novel perspective on the potential intervention mechanism of RCE in the treatment of AD.
A study on autonomous suturing task assignment in robot‐assisted minimally invasive surgery
Authors: Gao Shuai , Ji Shijun , Feng Mei , Lu Xiuquan , Tong Weihua 
Published: October 2020

AbstractBackgroundSutures are a set of standard actions which are accomplished by multi‐instruments, researchers studied the robot autonomy of suturing, which was based on movement planning completed by a single instrument, but did not consider the assignment of suturing tasks to instruments.MethodA method was proposed for the autonomous suturing task assignment to instruments, which built a comprehensive evaluation index under some constraint conditions to determine the optimal scheme of the suturing task assignment to instruments.ResultsAn experiment of duodenal ulcer repair with a suturing operation was conducted under the guidance of a surgeon, and the results showed that the optimal scheme of the suturing task assignment was obtained by using the proposed method.ConclusionsThe proposed method can be used for autonomous suturing task assignment, which is beneficial for improving the intelligence of robot operation.
Mechanistic Insights into the N‐Heterocyclic Carbene Catalyzed Synthesis of α,δ‐Diketones: A DFT Approach
Authors: Shyam Abhijit , Pradhan Amit K. , Mondal Paritosh 
Published: October 2020

AbstractDensity functional theory (DFT) has been utilized to understand the mechanism for N‐heterocyclic carbene (NHC) catalyzed synthesis of 1‐(4‐methoxyphenyl)‐2,4‐diphenylbutane‐1,4‐dione from p‐methoxybenzaldehyde and E‐chalcone. Quantum chemical simulations suggest that the catalytic cycle for the formation of α,δ‐diketone commences with nucleophilic interaction of in situ generated active catalyst NHC and p‐methoxybenzaldehyde to form a tetrahedral zwitterionic intermediate, which on subsequent proton transfer reactions result the Breslow intermediate. 1,4‐umpolung addition of Breslow intermediate to E‐chalcone followed by a proton shift facilitates the release of NHC from the catalytic cycle along with the generation of enolic form of the product. Enolic form finally transforms into the desired product under the assistance of both protonated and free 1,8‐diazabicyclo[5.4.0]undec‐7‐ene (DBU). Furthermore, conceptual DFT and frontier molecular orbital analyses help to unravel the role NHC in this catalytic cycle. Apart from NHC, protonated and free DBU, existence of advantageous π‐π stacking interaction is also very effective in lowering the activation energy barrier efficiently.
In search of positive mental health: Personality profiles and genetic polymorphisms
Authors: Nestor Paul G. , Hasler Victoria Choate , O'Donovan Keira , Lapp Hannah E. , Boodai Sara B. , Hunter Richard 
Published: October 2020

AbstractIndividuals vary greatly in their mental health and these differences may play a critical role in stress resistance, risk reduction and illness recovery. Here we ask how these differences may be related to normal variation in personality and genotype. One hundred healthy college students completed measures of mental health (Mental Health Continuum‐Short Form [MHC‐SF]), personality (NEO Five Factor Inventory) and adverse childhood experiences. Participants also provided saliva samples, genotyped for both the serotonin transporter (5‐HTTLPR) and the brain‐derived neurotrophic factor (BDNF), each assayed for naturally occurring polymorphisms, 5‐HTTLPR (short/long) and BDNF (valine/methionine). Mental health correlated strongly with the NEO triad of conscientiousness‐extraversion‐neuroticism, with largest contributions to MHC‐SF scores for conscientiousness, followed by extraversion and then neuroticism. The personality trait interaction of extraversion × conscientiousness uniquely accounted for approximately 44.22% 44.62% of the variance in MHC‐SF scores. Polygenic comparisons showed a significant gene × gene interaction, with highest mental health for 5‐HTTLPR‐S, Met carriers. Together these results provided support for distinct yet interacting roles of personality and genetics in the phenotypical expression of mental health.
Ru(bpy)32+‐Loaded Mesoporous Silica Nanoparticles as Electrochemiluminescent Probes of a Lateral Flow Immunosensor for Highly Sensitive and Quantitative Detection of Troponin I
Authors: Hong Donggu , Jo Eun‐Jung , Kim Kihyeun , Song Mun‐Beom , Kim Min‐Gon 
Published: October 2020

AbstractThe lateral flow immunosensor (LFI) is a widely used diagnostic tool for biomarker detection; however, its sensitivity is often insufficient for analyzing targets at low concentrations. Here, an electrochemiluminescent LFI (ECL‐LFI) is developed for highly sensitive detection of troponin I (TnI) using Ru(bpy)32+‐loaded mesoporous silica nanoparticles (RMSNs). A large amount of Ru(bpy)32+ is successfully loaded into the mesoporous silica nanoparticles with excellent loading capacity and shows strong ECL signals in reaction to tripropylamine. Antibody‐immobilized RMSNs are applied to detect TnI by fluorescence and ECL analysis after a sandwich immunoassay on the ECL‐LFI strip. The ECL‐LFI enables the highly sensitive detection of TnI‐spiked human serum within 20 min at femtomolar levels (≈0.81 pg mL−1) and with a wide dynamic range (0.001–100 ng mL−1), significantly outperforming conventional fluorescence detection (>3 orders of magnitude). Furthermore, TnI concentrations in 35 clinical serum samples across a low range (0.01–48.31 ng mL−1) are successfully quantified with an excellent linear correlation (R2 = 0.9915) using a clinical immunoassay analyzer. These results demonstrate the efficacy of this system as a high‐performance sensing strategy capable of capitalizing on future point‐of‐care testing markets for biomolecule detection.