bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic analysis of CFTR-impaired endothelial cells reveals a pro-inflammatory phenotype
Authors: Declercq Mathias , de Zeeuw Pauline , Conchinha Nadine V. , Geldhof Vincent , Ramalho Anabela S. , García-Caballero Melissa , Brepoels Katleen , Ensinck Marjolein , Carlon Marianne S. , Bird Matthew J. , Vinckier Stefan , Proesmans Marijke , Vermeulen François , Dupont Lieven , Ghesquière Bart , Dewerchin Mieke , Carmeliet Peter , Cassiman David , Treps Lucas , Eelen Guy , Witters Peter 
Published: November 2020

Cystic fibrosis (CF) is a life-threatening disorder characterised by decreased pulmonary mucociliary and pathogen clearance, and an exaggerated inflammatory response leading to progressive lung damage. CF is caused by bi-allelic pathogenic variants of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which encodes a chloride channel. CFTR is expressed in endothelial cells (ECs) and EC dysfunction has been reported in CF patients, but a role for this ion channel in ECs regarding CF disease progression is poorly described.We used an unbiased RNA sequencing approach in complementary models of CFTR silencing and blockade (by the CFTR inhibitor CFTRinh-172) in human ECs to characterise the changes upon CFTR impairment. Key findings were further validatedin vitroandin vivoin CFTR-knockout mice andex vivoin CF patient-derived ECs.Both models of CFTR impairment revealed that EC proliferation, migration and autophagy were downregulated. Remarkably though, defective CFTR function led to EC activation and a persisting pro-inflammatory state of the endothelium with increased leukocyte adhesion. Further validation in CFTR-knockout mice revealed enhanced leukocyte extravasation in lung and liver parenchyma associated with increased levels of EC activation markers. In addition, CF patient-derived ECs displayed increased EC activation markers and leukocyte adhesion, which was partially rescued by the CFTR modulators VX-770 and VX-809.Our integrated analysis thus suggests that ECs are no innocent bystanders in CF pathology, but rather may contribute to the exaggerated inflammatory phenotype, raising the question of whether normalisation of vascular inflammation might be a novel therapeutic strategy to ameliorate the disease severity of CF.
Asthma in South African adolescents: a time trend and risk factor analysis over two decades
Authors: Baard Cynthia B. , Franckling-Smith Zoe , Munro Jacinta , Workman Lesley , Zar Heather J. 
Published: November 2020

BackgroundSouth Africa has undergone major economic and health system changes, impacting the epidemiology of childhood asthma. This study aimed to investigate prevalence time trends of asthma in South African adolescents over two decades and to identify associated risk factors.MethodsA cross-sectional survey was conducted in 2017, in a randomised sample of 13–14-year-old Cape Town adolescents, using the standardised Global Asthma Network written, video and environmental questionnaires. Using time-trend analysis, the prevalence and severity of asthma were compared with data from the 2002 ISAAC phase III study. Environmental and social risk factors were analysed.ResultsA total of 3979 adolescents were included. The prevalence of lifetime and current asthma were 34.5% and 21.3%, respectively, on the self-report written questionnaire, similar to 2002 results. The prevalence of severe asthma in the previous 12 months increased, measured by wheeze limiting speech (7.8% to 11.8%), four or more attacks of wheezing (5.0% to 5.8%) or woken by wheeze on one or more nights per week (5.0% to 6.9%). The video questionnaire revealed increases in lifetime (16.9% to 22.5%), current (11.2% to 18.7%) and severe asthma (12.1% to 14.8%). Multivariate analysis showed associations between current asthma and smoking, female sex, pet exposure and higher socioeconomic status. Severe asthma was associated with smoking, pet exposure, outdoor pollution exposure and informal housing; 33% of those with severe or current asthma had been diagnosed.ConclusionThe prevalence of asthma is high, with increasing rates of severe asthma in adolescents. Underdiagnosis is a major concern and reduction in exposure to environmental factors, particularly smoking, and improved socioeconomic development are needed.
Home purchase restriction, real estate investment, and corporate innovation
Authors: Kuang Weida , Chen Changyu , Wang Qilin 
Published: November 2020

Abstract
It is ubiquitous for non-real estate firms to conduct real estate business in China. Home purchase restriction (HPR) affects corporate innovation by dampening the real estate investment of non-real estate firms. The extant literature has examined the impact of HPR on corporate innovation, but it has not focused on the expectation of HPR and the endogeneity problem. Employing a dataset of 1830 listed non-real estate firms over the period 2009–2016, this research explores the expectation of HPR on corporate innovation based on the motivations for real estate investment in non-real estate firms. We demonstrate that HPR facilitates the enhancement of research and development (R&D) investment in non-real estate listed firms by hindering real estate investment, particularly for non-high-tech firms. The effects of HPR arrive at the crest in the third implementation year and remain steady thereafter. The real estate investment of non-real estate firms rebounds and the R&D investment declines along with the cancellation of HPR. Tackling the selection bias and endogeneity problems, the baseline results are also robust. Hence, HPR should serve as a long-term vehicle to improving corporate innovation, in addition to preventing housing speculation.
Degradation and sorption of the herbicides 2,4-D and quizalofop-P-ethyl and their metabolites in soils from railway tracks
Authors: Buerge Ignaz J. , Pavlova Pavlina , Hanke Irene , Bächli Astrid , Poiger Thomas 
Published: November 2020

Abstract
Background
Broad spectrum of activity and low potential for leaching to groundwater are important arguments for the application of the herbicide glyphosate on railway tracks. Nevertheless, certain weeds are insufficiently controlled or develop resistance, and there is also an ongoing controversial discussion about possible carcinogenicity of glyphosate. Alternatives are thus strongly desired. 2,4-D and quizalofop-P-ethyl (QE) are two selective herbicides with a complementary spectrum of activity. When used in agriculture, the compounds and their metabolites exhibit low groundwater contamination potential. Uses on railway tracks may, however, be more critical, since degradation likely is slower and mobility higher than in agricultural soils. In this study, we investigated degradation and sorption of the two active substances as well as five metabolites in three soils collected from railway tracks and in a crushed sand, used for construction works.

Results
In these railway materials, the compounds were indeed degraded slower than in agricultural soils (mean half-lives differed by a factor of 1.4–26, depending on the substance) and mobility was higher (mean sorption coefficients differed by a factor of 3–19). Half-lives and sorption coefficients were also estimated by extrapolation of data from agricultural soils, considering the organic carbon content of railway soils and agricultural soils. Estimated values were more conservative than measured values.

Conclusions
Based on our experimental data, possible leaching to groundwater is predicted to be highest for 2,4-D and quizalofop-acid, the primary metabolite of QE, moderate for 2,4-dichlorophenol, the primary metabolite of 2,4-D, but low for QE. Secondary and tertiary metabolites were formed in only low quantities. For herbicides, for which no measured parameters are available for railway soils, estimated values may also be a viable alternative for a first tier groundwater assessment.
Deep learning based DNA:RNA triplex forming potential prediction
Authors: Zhang Yu , Long Yahui , Kwoh Chee Keong 
Published: November 2020

AbstractBackgroundLong non-coding RNAs (lncRNAs) can exert functions via forming triplex with DNA. The current methods in predicting the triplex formation mainly rely on mathematic statistic according to the base paring rules. However, these methods have two main limitations: (1) they identify a large number of triplex-forming lncRNAs, but the limited number of experimentally verified triplex-forming lncRNA indicates that maybe not all of them can form triplex in practice, and (2) their predictions only consider the theoretical relationship while lacking the features from the experimentally verified data.ResultsIn this work, we develop an integrated program named TriplexFPP (Triplex Forming Potential Prediction), which is the first machine learning model in DNA:RNA triplex prediction. TriplexFPP predicts the most likely triplex-forming lncRNAs and DNA sites based on the experimentally verified data, where the high-level features are learned by the convolutional neural networks. In the fivefold cross validation, the average values of Area Under the ROC curves and PRC curves for removed redundancy triplex-forming lncRNA dataset with threshold 0.8 are 0.9649 and 0.9996, and these two values for triplex DNA sites prediction are 0.8705 and 0.9671, respectively. Besides, we also briefly summarize thecisandtranstargeting of triplexes lncRNAs.ConclusionsThe TriplexFPP is able to predict the most likely triplex-forming lncRNAs from all the lncRNAs with computationally defined triplex forming capacities and the potential of a DNA site to become a triplex. It may provide insights to the exploration of lncRNA functions.
Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis
Authors: Yuan Songli , Ke Danxia , Li Rong , Li Xiangyong , Wang Lei , Chen Haifeng , Zhang Chanjuan , Huang Yi , Chen Limiao , Hao Qingnan , Yang Hongli , Cao Dong , Chen Shuilian , Guo Wei , Shan Zhihui , Yang Zhonglu , Zhang Xiaojuan , Qiu Dezhen , Guan Yuefeng , Zhou Xinan 
Published: November 2020

AbstractBackgroundPlant papain-like cysteine proteases (PLCPs) are a large class of proteolytic enzymes and play important roles in root nodule symbiosis (RNS), while the whole-genome studies ofPLCPfamily genes in legume are quite limited, and the roles ofGlycine max PLCPs(GmPLCPs) in nodulation, nodule development and senescence are not fully understood.ResultsIn the present study, we identified 97GmPLCPsand performed a genome-wide survey to explore the expansion of soybeanPLCPfamily genes and their relationships to RNS. Nineteen paralogous pairs of genomic segments, consisting of 77GmPLCPs, formed by whole-genome duplication (WGD) events were identified, showing a high degree of complexity in duplication. Phylogenetic analysis among different species showed that the lineage differentiation ofGmPLCPsoccurred after family expansion, and large tandem repeat segment were specifically in soybean. The expression patterns ofGmPLCPsin symbiosis-related tissues and nodules identified RNS-relatedGmPLCPsand provided insights into their putative symbiotic functions in soybean. The symbiotic function analyses showed that a RNS-relatedGmPLCPgene (Glyma.04G190700) really participate in nodulation and nodule development.ConclusionsOur findings improved our understanding of the functional diversity of legumePLCPfamily genes, and provided insights into the putative roles of the legumePLCPsin nodulation, nodule development and senescence.
Mortality and return to work in patients transported by emergency ambulance after involvement in a traffic accident
Authors: Ringgren Kristian Bundgaard , Mills Elisabeth Helen Anna , Christensen Erika Frischknecht , Mortensen Rikke Nørmark , Torp-Pedersen Christian , Kragholm Kristian Hay 
Published: November 2020

Abstract
Introduction/background
Traffic accidents constitute a common reason for injury. Little is known about long-term outcomes for patients following a traffic accident. Therefore, in this present paper, we examine 1-day, 30-day and 1-year mortality, and return to work (RTW) during a 1-year period.

Methods
Patients (between 18 and 65 years of age) who had an ambulance dispatched to them following a traffic accident and who were employed prior to the accident were identified from the Electronic Prehospital Emergency Patient (amPHI™) database in the North Denmark Region (catchment population ≈600,000) during 2006–2014. Outcomes of 1- and 30- and 365-day mortality and 1-year return to work (RTW), with mortality as competing risk. We stratified by intensive care unit (ICU) admission; and the anatomical region of injury (head/neck, thorax, abdomen, extremities and multiple injuries) is reported.

Results
Of 6072 patients in our study population, 59 (1%) died within 1 day and 76 (1.3%) within 30 days; 88 (1.5%) were dead within a year.
Thirty-day mortality was 1.7% for the 290 patients admitted to the ICU, and 1.2% for the remaining 5782 patients.
Within the study population, RTW rate was 92.7% (N = 5984). RTW was 84.8% among 290 ICU-admitted patients versus 93.1% for the remaining 5782 patients.
RTW rate was 94.6% for the 1793 patients discharged with a diagnosis of head/neck injury. Of 671 patients with a discharge diagnosis for the thoracic region, 92.6% returned to work. Of 402 patients with abdominal injury diagnoses, 90.8% returned to work. Of 1603 patients discharged with a diagnosis of extremity injury, the RTW rate was 93.6%. Of 192 patients with a discharge diagnosis of injury in multiple regions, 91.7% returned to work.

Conclusion
Overall, mortality rates were low and RTW rates high in patients who had an ambulance dispatched due to a traffic accident. Those admitted to the ICU had the lowest RTW rate, yet still around 80% returned to work.
Comparing multiple statistical software for multiple-indicator, multiple-cause modeling: an application of gender disparity in adult cognitive functioning using MIDUS II dataset
Authors: Chang Chi , Gardiner Joseph , Houang Richard , Yu Yan-Liang 
Published: November 2020

Abstract
Background
The multiple-indicator, multiple-cause model (MIMIC) incorporates covariates of interest in the factor analysis. It is a special case of structural equation modeling (SEM), which is modeled under latent variable framework. The MIMIC model provides rigorous results and becomes broadly available in multiple statistical software. The current study introduces the MIMIC model and how it can be implemented using statistical software packages SAS CALIS procedure, R lavaan package, and Mplus version 8.0.

Methods
In this paper, we first discussed the formulation of the MIMIC model with regard to model specification and identification. We then demonstrated the empirical application of the MIMIC model with the Midlife in the United States II (MIDUS II) Study (N = 4109) using SAS CALIS procedure, R lavaan package and Mplus version 8.0 to examine gender disparities in cognitive functioning. The input, output, and diagram syntaxes of the three statistical software packages were also presented.

Results
In terms of data structure, all three statistical programs can be conducted using both raw data and empirical covariance matrix. SAS and R are comprehensive statistical analytic packages and encompass numerous data manipulation capacities. Mplus is designed primarily for latent variable modeling and has far more modeling flexibility compared to SAS and R, but limited in data manipulation. Differences in model results from the three statistical programs are trivial. Overall, the results show that while men show better performance in executive function than women, women demonstrate better episodic memory than men.

Conclusions
Our study demonstrates the utility of the MIMIC model in its empirical application, fitted with three popular statistical software packages. Results from our models align with empirical findings from previous research. We provide coding procedures and examples with detailed explanations in the hopes of providing a concise tutorial for researchers and methodologists interested in incorporating latent constructs with multiple indicators and multiple covariates in their research projects. Future researchers are encouraged to adopt this flexible and rigorous modeling approach.
Management of syphilitic hepatitis
Authors: Kaya Abdurrahman , Kaya Sibel Yıldız 
Published: November 2020

AbstractSyphilis is a sexuality transmitted disease caused by Treponema pallidum. Liver involvement is very rarely seen and occurs in the second phase of the disease. Syphilitic hepatitis generally is mild clinical condition and is characterized by high serum alkaline phosphatase level, often with normal or only slightly abnormal transaminases. The skin eruptions are classically diffuse, symmetric maculopapular rashes involving trunk and extremities. Involvement of palms and soles is a strong clue to the diagnosis of secondary syphilis. Therefore, syphilitic hepatitis should be included in the early differential diagnosis in patient with abnormal liver enzyme, especially increased alkaline phosphatase, and rashes involving palms and soles.
Association among pre-pregnancy body mass index, gestational weight gain and neonatal birth weight: a prospective cohort study in China
Authors: Wang Yawen , Ma Haihui , Feng Yahui , Zhan Yongle , Wu Sansan , Cai Shuya , Shi Yingjie , Chen Yunli , Ma Liangkun , Jiang Yu 
Published: November 2020

Abstract
Background
This study aims to explore the relationships between pre-pregnancy body mass index (BMI), gestational weight gain (GWG), rate of GWG during the second and third trimesters (GWGrate) and birth weight among Chinese women.

Methods
Women were enrolled by 24 hospitals in 15 different provinces in mainland China from July 25th, 2017 to 26 November 2018. Pre-pregnancy BMI, GWG and GWGrate were calculated and divided in to different groups. The multinomial logistic regression model and restrictive cubic spline model were used to explore the relationships.

Results
Of the 3585 participants, women who were underweight, had insufficient GWG or GWGrate had 1.853-, 1850- or 1.524-fold higher risks for delivering small-for-gestational-age (SGA) infant compared with women who had normal BMI, sufficient GWG or GWGrate. Women who were overweight/obese, had excessive GWG or GWGrate had 1.996-, 1676- or 1.673-fold higher risks for delivering large-for-gestational-age (LGA) infant. The effects of GWG and GWGrate on birth weight varied by pre-pregnancy BMI statuses. Dose-response analysis demonstrated L-shaped and S-shaped relationships between pre-pregnancy BMI, GWG, GWGrate and neonatal birth weight.

Conclusions
Pre-pregnancy BMI, GWG or GWGrate were associated with neonatal birth weight among Chinese women. Both body weight before and during pregnancy should be maintained within the recommendations to prevent abnormal birth weight.
Successful emergency endovascular aortic repair for intratumoral hemorrhage in extensive retroperitoneal mass of testicular origin
Authors: Hulova S. , Aziri R. , Vulev I. , Palacka P. , Kolnikova G. , Rejlekova K. , Chovanec M. , Mardiak J. , Pindak D. , Mego M. 
Published: November 2020

AbstractBackgroundMetastatic germ cell cancer of the testis is characterized by favorable prognosis since effective treatment methods are available even in cases of extensive disease. Retroperitoneal masses frequently encroach major blood vessels requiring a vascular intervention usually performed in association with the post-chemotherapy retroperitoneal lymph node dissection (RPLND). Reported clinical case describes a successful pre-treatment endovascular surgery for abdominal aortic rupture allowing for full-dose systemic chemotherapy administration, and subsequent radical surgical intervention at primary tumor site as well as metastatic retroperitoneal lymph node dissection including the reconstruction of inferior caval vein.Case presentationPatient presented with left-sided testicular tumor and voluminous retroperitoneal mass with vascular involvement. Soon after the patient had been admitted for the first cycle of cisplatin-based chemotherapy, computed tomographic angiography (CTA) revealed a dorsal aortic wall rupture with active extravasation and irregular pseudoaneurysmatic dilatation of the aorta below the leak area. Retroperitoneal intratumoral hemorrhage associated with the bilateral iliac venous thrombosis required an endovascular repair procedure of infrarenal abdominal aorta.ConclusionsFollowing the successful endovascular aortic repair 3 cycles of BEP (bleomycin, etoposide, cisplatin) regimen were administered with subsequent delayed left radical orchiectomy and RPLND associated with vena cava inferior (VCI) resection. Reconstruction of VCI was originally not deemed necessary as collateral blood flow appeared sufficient, however, intraoperative complications resulted in the need for unilateral VCI reconstruction, using the interposed bypass between right common iliac vein and infrarenal segment of VCI. Histopathologic examination of the attained specimen detected no vital cancer structures. The patient remains disease-free 18 months after the RPLND.
Expression of early angiogenesis indicators in mature versus immature teeth
Authors: Caviedes-Bucheli Javier , Lopez-Moncayo Luis F. , Muñoz-Alvear Hernan Dario , Hernandez-Acosta Francisco , Pantoja-Mora Melissa , Rodriguez–Guerrero Angie S. , López–Ordoñez Alexander , Díaz Luis E. , Gomez-Sosa Jose Francisco , Munoz Hugo R. 
Published: November 2020

Abstract
Background
Proper oxygen balance in the dental pulp is essential for cell metabolism. Angiogenesis in the pulp is a constant process during the life of the tooth. Hypoxia indicators in a tissue, such as HIF-1α, as well as vascular destabilization markers, such as ANG2 and its receptor TIE2, are necessary for angiogenesis. Therefore the purpose of this study is to evaluate the expression of HIF-1α, ANG1, ANG2 and TIE2 in dental pulp as early angiogenesis indicators in teeth with complete and incomplete root development.

Methods
Forty human dental pulps were obtained from freshly extracted third molars divided into two groups: incomplete (n = 20) and complete (n = 20) root development. Dental pulps were stored at − 80 °C, defrosted in an ice bath and re-frozen with their respective thaws to disintegrate the tissue. Three sonication cycles were performed until the tissues were homogenized, then thaw were centrifuged and the supernatant was collected for the detection of the markers to be studied. The samples were processed for the ELISA test using the ELISA-sandwich principle. Student t and Mann–Whitney U tests were performed to determine statistically significant differences between groups.

Results
In the complete root development, HIF-1α, ANG1, ANG2 and TIE2 expressions were significantly higher than their expression in the incomplete root development group.

Conclusions
The angiogenic process seems to be a physiological process in the dental pulp. Angiogenic activity is higher in teeth with mature than immature apex teeth.