bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Nutritional Status for Some Sensitive Sets and its Relationship to Natural Antioxidants
Authors: Sami Rokayya A. , Khojah Ebtihal Y. , Elgarni Eman A. , Benajiba Nada 
Published: November 2019

There exists a need to get the knowledge of natural antioxidants’ sources as a result of several factors; perhaps most notably is the change in our lifestyle. Although modern life has become easier and more comfortable, it harms us in diff erent ways. So, we should look for the best ways to support our health. Although our body manufactures antioxidants, we need additional quantities for protecting the body. Natural antioxidants are found in fresh vegetables, fruits, seafood and some nuts. Sport is essential for our health. The short periodsports could increase the antioxidant production quickly too. Groups of children, adolescents, adults and elderly have been tested. A selfadministered questionnaire asking about socio-demographic data, various forms of activities, dietary intakes and habits was used for data collection. The elementary schoolchildren were interviewed by structured interview to gather information. Therefore, there is a necessity to develop a nutrition education program in order to change lifestyle habits and avoid the negative health consequences.
The Effects of Loading Frequency, Sensitization, and Crack Length on Corrosion Fatigue of AA5456-H116
Authors: Schrock D.J. , (Warner) Locke J.S. 
Published: November 2019

The effects of fatigue loading frequency (f), sensitization, and crack length on corrosion fatigue crack growth rates (da/dN) were investigated for AA5456-H116 under full immersion in 3.5 wt% NaCl. Results from fracture mechanics-based experiments conducted at a constant stress-intensity range (ΔK) and load ratio (R) suggest that highly sensitized AA5456-H116 microstructures (ASTM G67 nitric acid mass loss tests [NAMLT] value of 24 mg/cm2 and higher) exhibit increased da/dN over microstructures in the as-received condition (NAMLT 5 mg/cm2) and the onset of an inverse f-dependence. For a single, high level of sensitization (70 mg/cm2), da/dN increased 3.5× as f decreased from 10 Hz to 0.03 Hz. At a singular low loading f of 0.03 Hz, high sensitization levels (65 mg/cm2) accelerated da/dN fivefold over da/dN in the as-received condition. The da/dN of microstructures below a critical NAMLT value of 24 mg/cm2 were f-independent. Specifically, in microstructures with a low sensitization level (ASTM G67 NAMLT value less than 24 mg/cm2), there was no increase in da/dN as f decreased from 1 Hz to 0.03 Hz. At any singular f, sensitization up to 24 mg/cm2 did not accelerate da/dN over the rate in an as-received microstructure. Additional testing established that the inverse f-dependence of da/dN observed in highly sensitized microstructures cannot be attributed to crack length effects. Two hypotheses are discussed that may explain the observed inverse relationship between f and da/dN in microstructures at or above the critical NAMLT value of 24 mg/cm2.
Angiopoietin-2 as a biomarker of non-ST-segment elevation myocardial infarction in patients with or without type 2 diabetes
Authors: Skowerski Tomasz , Nabrdalik Katarzyna , Kwiendacz Hanna , Pajak Maciej , Mizia-Stec Katarzyna , Gasior Zbigniew , Gumprecht Janusz 
Published: November 2019

IntroductionAngiopoietin-2 (Ang-2) is a novel marker of coronary artery disease (CAD) and diabetes (DM). The aim was to evaluate Ang-2 as a potential new biomarker of non-ST elevation myocardial infarction (NSTEMI) in patients with or without type 2 DM (T2DM).Material and methodsThis was a multi-center, prospective study that included 138 (males: 91/66%) consecutive patients hospitalized due to NSTEMI, T2DM, or different cardiac disorders. The subjects were divided into four study groups: group A: 28 patients with NSTEMI and T2DM; group B: 47 patients with NSTEMI without T2DM; group C: 31 patients with T2DM, without a history of CAD; group D: 32 patients as a control group. Patients with NSTEMI underwent urgent coronarography. Clinical characteristics including biomarkers (hs-CRP, hsTnT, NT-proBNP, VEGF, HbA1c), SYNTAX SCORE, type of intervention (PCI vs. CABG), and number of implanted stents were taken into account in the analysis.ResultsSerum Ang-2 concentrations were significantly higher in patients with NSTEMI (group A: 1769 pg/ml; group B: 1757 pg/ml) and patients with T2DM (group C: 1993 pg/ml) as compared to the patients without CAD and without T2DM (group D: 866.8 pg/ml; p < 0.05). The prognostic accuracy of Ang-2 in NSTEMI diagnosis was determined with the area under the ROC curve (area under curve (AUC) = 0.63).ConclusionsAngiopoietin-2 serum concentration is elevated in the presence of NSTEMI in patients with and without T2DM and does not correspond to the degree of myocardial injury and hemodynamic status. Ang-2 remains elevated also in patients with T2DM without a history of CAD.
Activation of CREB-binding protein ameliorates spinal cord injury in tabersonine treatment by suppressing NLRP3/Notch signaling
Authors: Chuan Yu , Wang Yu , Jin Xu , Ming Shi , Bing Wan , Kai Wu , Xiang Chen , Kun Peng 
Published: November 2019

IntroductionSpinal cord injury (SCI) alters the integrity of the spinal cord, which leads to loss of multiple organs’ function including locomotor function. The present study evaluates the protective effect of tabersonine against SCI.Material and methodsSCI was induced by traumatic injury and animals were treated with tabersonine 20 and 40 mg/kg, intraperitoneally for the period of 10 days. Tabersonine’s effect was determined by estimating locomotor and neurological function in spinal cord injured rats. Moreover, mediators of inflammation were estimated using enzyme-linked immunosorbent assay (ELISA) and the effect of tabersonine on Notch/inflammasome signaling was estimated by reverse transcription polymerase chain reaction (RT-PCR), western blot assay and immunohistochemistry. Apoptosis of neuronal cells was estimated by staining with Nissl stain on spinal cord tissue in SCI rats.ResultsData of the study suggest that neurological and motor functions were improved in the tabersonine treated group compared to the spinal cord injured (SI) group. There was a decrease in the mediators of inflammation in the spinal cord tissue of the tabersonine treated group compared to the SI group. Treatment with tabersonine ameliorates the altered expression of NICD, Nestin and Hes-1 protein and mRNA expression of Notch-1 and Hes-1 in the SCI rats. It was also observed that the tabersonine treated group showed activation of CREB and inhibition of the NLRP-3 pathway in SCI rats. Moreover, apoptosis of neuronal cells was reduced in the tabersonine treated group compared to the SI group.ConclusionsData of the investigation suggest that tabersonine protects against spinal cord injury by activating CREB and reducing NLRP3/Notch signaling.
Analysis of master transcription factors related to Parkinson’s disease through the gene transcription regulatory network
Authors: Wei Li , He Fei , Zhang Wen , Chen Wenhua , Yu Bo 
Published: November 2019

IntroductionThis study investigated the relationships between differentially co-expressed gene pairs or links (DCLs) and transcription factors (TFs) in the gene transcription regulatory network (GTRN) to clarify the molecular mechanisms underlying the pathogenesis of Parkinson’s disease (PD).Material and methodsMicroarray dataset GSE7621 from Gene Expression Omnibus (GEO) was used to identify differentially expressed genes (DEGs) and perform Gene Ontology (GO) enrichment analysis. Differentially co-expressed genes (DCGs) and DCLs were identified by the DCGL package in R soft-ware. DCLs that were potentially related to the regulation mechanisms, and corresponding TFs, were identified using the DR sort function in the DCGL V2.0 package. The GTRN was constructed with these DCLs-TFs, and visualized with Cytoscape software.ResultsA total of 131 DEGs, including 77 up-regulated DEGs and 54 down-regulated DEGs, were identified, which were mainly enriched for plasma membrane, cell activities, and metabolism. We found that ICAM1-LTBP and CTHRC1-UTP3 might alter gene regulation relationships in PD. The GTRN was constructed with DCLs-TFs, including 348 nodes (118 TFs and 230 DCGs) and 1045 DCLs. These TFs (AHR, SP1, PAX5, etc.) could regulate many target genes (e.g. ICAM1 and LTBP) in the GTRN of PD.ConclusionsICAM1 and LTBP may play a role in PD symptom development and pathology, and might be regulated by important TFs (AHR, SP1, PAX5, etc.) identified in the GTRN of PD. These findings may help elucidate the molecular mechanisms underlying PD and find a novel drug target for this disease.
Long non-coding RNA LINC00336 as an independent prognostic indicator and an oncogenic lncRNA in bladder cancer
Authors: Guo Xi , Liu Naijiang , Liu Mingkai 
Published: November 2019

IntroductionLong non-coding RNAs (lncRNAs) have been implicated in the initiation and progression of malignant tumor. The aim of the present study was to investigate whether LINC00336 contributes to the tumorigenesis of bladder cancer (BCa).Material and methodsLncRNA expression profiling in BCa tissues was analyzed using lncRNA microarray. Cell viability, invasion and apoptosis were detected using CCK8, Transwell and Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC) double staining, respectively.ResultsLncRNA microarray and RT-qPCR revealed that LINC00336 was significantly up-regulated in BCa tissues and cell lines compared with the corresponding control group. Kaplan-Meier and multivariate regression analysis demonstrated that LINC00336 could serve as an independent risk factor for predicting the prognosis of BCa patients. In vitro experimental measurements showed that knockdown of LINC00336 had the ability to block cell proliferation and invasion, as well as to induce cell cycle arrest and apoptosis.ConclusionsThese findings indicated that LINC00336 might serve as an oncogene in the initiation and progression of BCa, and LINC00336 may be a valuable and promising therapeutic target for the treatment of BCa.
Low expression of microRNA-125b enhances the expression of STAT3 and contributes to cholesteatoma growth
Authors: Zang Jian , Yang Bo , Feng Shuai , Jiang Xuejun 
Published: November 2019

IntroductionMicroRNA-125b has been found to be down-regulated in many types of malignant tumours and diseases with excessive proliferation of keratinocytes, such as cutaneous squamous cell carcinoma and psoriasis. Cholesteatoma, which is mainly composed of keratinocytes, also has characteristics of abnormal proliferation similar to a malignant tumour. However, the expression and regulatory mechanisms of miR-125b and its downstream genes in cholesteatoma have not been clarified.Material and methodsReal time fluorescence quantitative PCR was applied to detect the expression of miR-125b in the cholesteatoma and corresponding retroauricular skin. Immunohistochemical staining and western blot were used to detect signal transducers and activators of transcription 3 (STAT3) and the downstream gene cyclinD1, survivin, and vascular endothelial growth factor (VEGF) in the cholesteatoma and corresponding retroauricular skin. The targeted regulatory relationship between miR-125b and STAT3 was confirmed by dual luciferase reporter assay. Proliferation and apoptosis of transfected HaCaT cells were detected by MTS, cell cycle, and apoptosis assays.ResultsWe observed down-regulation of miR-125b and up-regulation of STAT3, cyclinD1, survivin, and VEGF in cholesteatoma tissues. STAT3 was a direct target gene of miR-125b. Inhibition of miR-125b enhanced STAT3 and its downstream genes expression, promoted HaCaT cell proliferation, and inhibited apoptosis.ConclusionsThe results of this study demonstrate that miR-125b can influence the growth of cholesteatoma by targeting STAT3 and its downstream genes, including cyclinD1, survivin, and VEGF, thus providing an opportunity to establish new medical therapy strategies and facilitating further study of the pathogenesis of cholesteatoma.
The identification and preliminary study of lncRNA TUG1 and its related genes in hepatocellular carcinoma
Authors: Li Lei , Liu Shuiping , Peng Li , Zhang Yapen , Zhang Yaqin , Zeng Haiyan , Li Guanchen , Zhang Chaoyang 
Published: November 2019

IntroductionHepatocellular carcinoma (HCC) is a common malignant tumour of the digestive system, which is a threat to public health. The purpose of this study was to investigate the featured genes and pathways of HCC from a bioinformatics database, and verify their correlation with diagnosis and prognosis of HCC.Material and methodsWe downloaded the gene expression profile on HCC from the Gene Expression Omnibus (GEO), and software R was used to identify differentially expressed lncRNA (DEL). The target genes of the lncRNA were further predicted by using a cluster database and molecular interaction database. Subsequently, a combined interaction network of target genes was constructed using the Cytoscape platform with preliminary verification at the level of different databases, cell lines, and tissues. Finally, we explored the effectiveness of TUG1 and its target genes on the diagnosis and prognosis of HCC by univariate Cox analysis and survival analysis.ResultsA total of four DELs were identified and the most remarkably up-regulated lncRNA was TUG1, which included 12 high-confidence target genes. Moreover, we found that the expression changes of TUG1 and its target genes in different databases, cell lines, and liver cancer tissues were consistent with the prediction. The high expression of TUG1 and its target genes could significantly predict the shorter survival time of HCC patients, among which NCAPG, MCM6, PIGC, PEA15, and RACGAP1 have significant diagnostic value for HCC (AUC > 0.9).ConclusionsThis study provides a starting point for the screening of therapeutically relevant targets in HCC. Further experiment should be conducted to verify our findings.
Expression differences between proteins responsible for DNA damage repair according to the Gleason grade as a new heterogeneity marker in prostate cancer
Authors: Jaworski Damian , Gzil Arkadiusz , Antosik Paulina , Zarębska Izabela , Dominiak Joanna , Neska-Długosz Izabela , Kasperska Anna , Grzanka Dariusz , Szylberg Łukasz 
Published: November 2019

IntroductionThe purpose of this research was to explore the correlation between Gleason score and pattern and the expression of the MLH1, MSH2, MDC1, TP53BP1 proteins in prostate cancer (PC). Prostate cancer development is related to errors in DNA, among others double-strand breaks (DSB) and changes in the base sequence of the DNA. These errors should be repaired through mismatch (MMR) or DSB repair proteins such as MSH2, MLH1, MDC1 and TP53BP1.Material and methodsA total of 500 prostate cancer specimens were recruited in this study. From among all gathered specimens the 52 most suitable cases were selected. The expression of examined proteins was detected by immunohistochemistry, and its correlation with the Gleason score and pattern were further analyzed through standard statistical algorithms.ResultsThe results show a significant correlation between Gleason pattern and the nuclear expression of the MSH2 protein and the cytoplasmic expression of the MLH1 protein. Gleason score significantly correlates with the nuclear and the cytoplasmic expression of the MSH2 protein and the cyto­plasmic expression of the MDC1 protein. There is no correlation between the nuclear or cytoplasmic expression of the TP53BP1 protein and Gleason pattern or score.ConclusionsOur study suggests that the aberration in the MMR repair mechanism may be significantly more important regarding the grading among PC cells in comparison to the impact of alterations in the DSB repair mechanism. The lack of correlation between expression of the TP53BP1 protein and Gleason pattern and Gleason score suggests that the radiation resistance of PC is independent of alterations connected with TP53BP1.
Does concomitance of Hashimoto’s disease and type 1 diabetes affect diabetes control and development of its complications?
Authors: Szcześniak Grzegorz , Kozak-Nurczyk Patrycja , Dziemidok Piotr 
Published: November 2019

IntroductionAutoimmune diseases concomitant with diabetes may complicate the treatment and adversely affect the prognosis. The most common is Hashimoto’s disease (HD). We compared diabetes control and prevalence of chronic complications in type 1 diabetes patients differing in the coexistence of HD.Material and methodsMedical records of 188 type 1 diabetics were analysed. Hashimoto’s disease was diagnosed based on medical history, as well as determination of the levels of thyroid peroxidase antibodies, hormones and ultrasound exa¬mination. Statistical analysis was performed using Statistica 10PL.ResultsHD was diagnosed in 43 patients (23%). The mean HbA<sub>1c</sub> was 8.8 ±1.5% in the group with HD, and 9 ±1.6% in the group without HD (ns). The prevalence of diabetes complications was similar in both groups: ischae¬mic heart disease was diagnosed in 19% of patients with HD and 19% without HD, cerebral vascular insufficiency – 8% and 7%, peripheral neuropathy – 14% and 12%, sensory polyneuropathy – 47% and 46%, diabetic foot – 7% and 8%, Charcot osteoarthropathy – 7% and 2%, cardiovascular neuropathy – 21% and 28%, neuropathy of the gastrointestinal tract – 5% and 6%, nephropathy – 12% and 19%, retinopathy – 42% and 43%, and cataract in 28% and 19%, respectively. Impaired hypoglycaemia perception was rarer in the group with HD: 9% vs. 25% (p ≈0.04).ConclusionsHashimoto’s disease does not significantly affect the level of type 1 diabetes control or the development of its complications. Only autonomic neuropathy in the form of impaired awareness of hypoglycaemia is rarer in patients with that thyroiditis.
A new model of the coupled carbon, nitrogen, and phosphorus cycles in the terrestrial biosphere (QUINCY v1.0; revision 1996)
Authors: Thum Tea , Caldararu Silvia , Engel Jan , Kern Melanie , Pallandt Marleen , Schnur Reiner , Yu Lin , Zaehle Sönke 
Published: November 2019

Abstract. The dynamics of terrestrial ecosystems are shaped by the coupled cycles of carbon, nitrogen, and phosphorus, and these cycles are strongly dependent on the availability of water and energy. These interactions shape future terrestrial biosphere responses to global change. Here, we present a new terrestrial ecosystem model, QUINCY (QUantifying Interactions between terrestrial Nutrient CYcles and the climate system), which has been designed from scratch to allow for a seamless integration of the fully coupled carbon, nitrogen, and phosphorus cycles with each other and also with processes affecting the energy and water balances in terrestrial ecosystems. This new model includes (i) a representation of plant growth which separates source (e.g. photosynthesis) and sink (growth rate of individual tissues, constrained by temperature and the availability of water and nutrients) processes; (ii) the acclimation of many ecophysiological processes to meteorological conditions and/or nutrient availability; (iii) an explicit representation of vertical soil processes to separate litter and soil organic matter dynamics; (iv) a range of new diagnostics (leaf chlorophyll content; 13C, 14C, and 15N isotope tracers) to allow for a more in-depth model evaluation. In this paper, we present the model structure and provide an assessment of its performance against a range of observations from global-scale ecosystem monitoring networks. We demonstrate that QUINCY v1.0 is capable of simulating ecosystem dynamics across a wide climate gradient, as well as across different plant functional types. We further provide an assessment of the sensitivity of key model predictions to the model's parameterisation. This work lays the ground for future studies to test individual process hypotheses using the QUINCY v1.0 framework in the light of ecosystem manipulation observations, as well as global applications to investigate the large-scale consequences of nutrient-cycle interactions for projections of terrestrial biosphere dynamics.
Towards an automatic early warning system of flood hazards based on precipitation forecast: the case of the Miño River (NW Spain)
Authors: González-Cao José , García-Feal Orlando , Fernández-Nóvoa Diego , Domínguez-Alonso José Manuel , Gómez-Gesteira Moncho 
Published: November 2019

Abstract. An early warning system for flood prediction based on
precipitation forecast is presented. The system uses rainfall forecast
provided by MeteoGalicia in combination with a hydrologic (Hydrologic Modeling System, HEC-HMS) and a
hydraulic (Iber+) model. The upper reach of the Miño River and the
city of Lugo (NW Spain) are used as a study area. Starting from rainfall
forecast, HEC-HMS calculates the streamflow and Iber+ is automatically
executed for some previously defined risk areas when a certain threshold is
exceeded. The analysis based on historical extreme events shows that the
system can provide accurate results in less than 1 h for a forecast
horizon of 3 d and report an alert situation to decision makers.
Epistemologías del Sur y las Economías de Ellas. La Cooperativa “Capuchinhas” de la Sierra de Montemuro
Authors: Cunha Teresa , Sousa Vanessa 
Published: November 2019

La cooperativa “Capuchinhas” es una iniciativa de mujeres de Serra do Montemuro, una de las regiones más aisladas y empobrecidas del centro de Portugal. Desde su creación en la década de 1980, esta cooperativa ha sido ideada como forma de resistencia al empobrecimiento y al aislamiento geográfico. Por otro lado, se ha convertido en una alternativa local para la creación de empleo y la emancipación de las mujeres. Esta cooperativa presta especial atención a los problemas concretos de su región y su población, y busca afrontar los desequilibrios territoriales y la creciente mercantilización de todos los ámbitos de la vida. Nuestro trabajo tiene dos objetivos principales. El primero es la expansión teórica y analítica del concepto de economía feminista. En segundo lugar, a través de una etnografía densa, presentamos la complejidad del establecimiento y la vida de la cooperativa, dando centralidad a las propias palabras y narrativas de las protagonistas que piensan en sí mismas y en el mundo en el que viven y trabajan.
The correlation of intake phytate and tannin on serum transferrin receptor and hemoglobin in stunted overweight adolescents
Authors: Mani La , Fatimah-Muis Siti , Kartini Apoina 
Published: November 2019

Stunted overweight teenagers are at risk of having iron deficiency. Iron deficiency is caused by various factors including the high food absorption inhibitors of iron such as phytate and tannins. Phytate and tannin contain polyphenol compounds which have a strong ability to bind iron so that it inhibits iron absorption in the intestine. This study aims to analyze the correlation between phytate, tannin intake and serum transferrin receptor (sTfR) and hemoglobin in stunted overweight adolescents. The research method was a cross-sectional study of 64 stunted overweight adolescents selected by consecutive sampling in four high schools/vocational high schools in Banyumanik District, Semarang City. Phytate and tannin intake data using SQ-FFQ method. The serum transferrin receptor examination uses the ELISA method and the hemoglobin level uses the Cyanomethemoglobin method. The results of the study, most of the respondents had high phytate and tannin intake of 96.9% and 89.1%. Respondents with low serum transferrin receptor were 7.8% and low hemoglobin levels were 7.8%. There was no correlation between phytate intake with serum transferrin receptor or hemoglobin (p1 = 0.937 r1 = -0.010, p2 = 0.192 r2 = 0.165). Tannins were significantly correlated with serum transferrin receptor and hemoglobin (p1 = 0.005 r1 = 0.344, p2 = 0.002 r2 = -0.374). Based on multivariate analysis, tannin is a determinant of hemoglobin (R2 = 0.257). Conclusion is that tannin is positively correlated with serum transferrin receptor and hemoglobin in stunted overweight adolescents. Excessive tannin intake can cause deficiency in stunted overweight adolescents.