bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircFAM114A2 Inhibits Bladder Cancer Proliferation and Promotes Cisplatin Sensitivity via the miR-222-3p/P27 and miR-146a-5p/P21 Cascades
Authors: Lv Jiancheng , Zhou Zijian , Wang Jingzi , Yang Xiao , Yu Hao , Han Jie , Feng Dexiang , Yuan Baorui , Wu Qikai , Li Pengchao , Lu Qiang , Yang Haiwei 
Published: November 2020

Abstract
Background: Circular RNAs (circRNAs) are noncoding RNAs that have the structure of a covalently closed loop. Increasing data has proved that circRNA can influence the development and progression of tumors. CircFAM114A2 is generated from several exons of FAM114A2. However, the function and mechanisms of circFAM114A2 in bladder cancer (BCa) remain unclear. This research aimed to reveal that circFAM114A2 inhibits bladder cancer progression and improves sensitivity of cisplatin chemotherapy by inducing G1/S cell cycle arrest via novel miR-222-3p/P27 and miR-146a-5p/P21 cascades.Methods: Here, to elucidate the potential roles of circFAM114A2 in BCa, we conducted RNA-sequencing on 5 pairs of BCa samples and screened for circRNAs. CircRNAs, microRNAs (miRNAs) and mRNAs, as well as levels of P27 and P21, in human cells and tissues were detected by qRT-PCR and western blot, respectively. CircRNA-miRNA interactions and miRNA-downstream mRNAs interactions were investigated by RNA pull-down assay and fluorescence in situ hybridization (FISH) or luciferase reporter assays, respectively. Then, the function of circFAM114A2 in BCa was explored using cell proliferation, cell cycle and tumorigenesis assays in nude mice. Finally, the function of circFAM114A2 in cisplatin chemo-sensitivity in BCa was detected by IC50 and tumor formation of xenograft in cisplatin-treated nude mice. Results: We discovered that circFAM114A2 levels were decreased in BCa cell lines and tissues. According to follow-up data, BCa patients with higher circFAM114A2 expression had better survival. Importantly, the levels of circFAM114A2 were associated with the histological grade of BCa. Overexpression of circFAM114A2 inhibited cell proliferation and increased sensitivity to cisplatin chemotherapy. Mechanistically, circFAM114A2 directly sponged miR-222-3p/miR-146a-5p and subsequently influenced the expression of the downstream target genes P27/P21, which, in turn, inhibited progression of BCa.Conclusions: CircFAM114A2 acted as a tumor suppressor through a novel circFAM114A2/miR-222-3p/P27 and circFAM114A2/miR-146a-5p/P21 pathway. CircFAM1142 has therefore great potential as a prognostic biomarker and therapeutic target for BCa.
Method to Determine the Standard Deviation of SUV Parameters
Authors: Luca Giulia Maria Rita De , Habraken Jan 
Published: November 2020

Abstract
Some of the parameters used for the quantification of Positron Emission Tomography (PET) images are the Standardized Uptake Value (SUV)Max, SUV Mean and SUV Peak. In order to assess the significance of an increasing or decreasing of these parameters for diagnostic purposes it is relevant to determine their standard deviation. In this study we present a method to determine the range of statistical variation of the SUV in PET images. Our method is based on dividing an original dataset into subsets of shorter time-frames. The variation between the SUV parameters of the subsets is used to estimate the standard deviation of the of the original acquisition. This method was tested on images of a NEMA quality phantom with acquisition time of 150 s per bed position and foreground to background activity ratio of F18-2-fluoro-2-deoxy-D-glucose (FDG) of 10:1. This original dataset has been reconstructed with different reconstruction lengths, generating new data subsets. The SUV Max, Mean and Peak were calculated for each image in the subsets. Their standard deviation has been calculated per subset for the different spheres included in the phantom. The variation of each subset has then been used to estimate the expected variation between images at 150 s reconstruction length. We report the largest standard deviation of the SUV parameters for the smallest sphere, and the smallest variation for the largest sphere. The expected variation at 150 s reconstruction length does not exceed 6% for the smallest sphere and 2% for the largest sphere, but we report an higher coefficient of variation (up to 30%) for shorter reconstruction lengths. We also report significant differences in the variation of SUV parameters for the larger spheres. With the presented method we are able to determine the standard deviation of SUV parameters only due to and the statistical variation. The method enables us to evaluate the effect of parameter selection and lesion size on the standard deviation, and therefore to evaluate its relevance on the total variation of the SUV value between studies.
Isolation and identification of serum exosomes and correlation of its contents with acute ischemic stroke
Authors: Yu Pei , Chen Wencheng 
Published: November 2020

Abstract
Background

Erythrocyte deformability is one of the pathophysiological changes of high-risk factors such as smoking, hypertension and atherosclerosis in stroke. It mainly affects blood viscosity and fluidity in the occurrence, development and outcome of the disease. Exosomes are a new type of biological activity test target, and it mainly contents miRNA, but its effect on stroke is still not clear.
Objective

To detect the serum exosome-derived miR-150-5p expression in patients with acute ischemic stroke and explore its diagnostic potential for acute ischemic stroke.
Methods

A total of 84 samples were collected with matched age and gender, collecting relevant laboratory indicators and general clinical data of the research subjects. The kit was used to extract serum exosomes, real-time fluorescent quantitative polymerase chain reaction was used to determine the expression of serum exosomal miR-150-5p, evaluate its value as a diagnostic marker through the ROC curve. Through Randa, Target Scan and other online databases, bioinformatics methods predict the target genes of miR-150, the results are drawn Venny to take intersection analysis, literature screening and ischemic stroke related target genes.
Results

The exosomes showed elliptical or round membranous vesicles with a diameter between 30–200 nm and fusion phenomenon and detected the exosomal marker proteins CD63 and HSP70; Compared with the control group, the relative expression of exosomal miR-150-5p in patients with acute ischemic stroke was increased (T = 8, P < 0.001). The expression of serum exosomal miR-150-5p in the disease group at different time points after stroke, the difference was not statistically significant(Chi-square = 2.925, P = 0.232); the area under the ROC curve was 0.883. bioinformatics prediction analysis of miR-150 target genes, which may be involved in the development of stroke disease through EGR2 and PLP2, as well as erythrocyte membrane protein-related genes SLC4A1 and SPTB also belong to the miR-150 prediction targets.
Conclusion

The expression of exosome-derived miR-150-5p is relatively high in patients with acute stroke, and it has a certain potential for early disease diagnosis.
Modulation of Plasma Sphingosine-1-phosphate Levels via Dietary Salt Intervention in Chinese Adults: An Intervention Trial
Authors: Ma Qiong , Chu Chao , Xue Yanbo , Yan Yu , Hu Jiawen , Zheng Wenling , Wang Keke , Wang Yang , Yuan Yue , Liao Yueyuan , Chen Ph.D. Chen , Mu Jianjun 
Published: November 2020

Abstract
Background: Salt is a crucial factor for blood pressure modulation, especially in salt-sensitive individuals. Sphingosine-1-phosphate (S1P), a pleiotropic bioactive sphingolipid metabolite participating in blood pressure regulation, has recently been identified as a novel lipid diuretic factor. However, the relationships among salt intake, circulating S1P levels, and blood pressure changes in human beings are unknown. Thus, we conducted this intervention trial to explore the effect of dietary salt intake on plasma S1P levels and to examine the relationship between S1P and blood pressure in Chinese adults.Methods: 42 participants (aged 18–65 years) were recruited from a rural community in Shaanxi, China. All participants first maintained their normal diet for 3 days, then sequentially ate a low-sodium diet (3.0 g/day NaCl) for 7 days, followed by a high-sodium diet (18.0 g/day NaCl) for 7 days. We assessed their plasma S1P concentrations on the last day of each intervention phase by liquid chromatography-tandem mass spectrometry. We classified the subjects who demonstrated at least a 10% increase in mean arterial pressure upon transitioning from a low-salt to a high-salt diet as salt-sensitive and the others as salt-resistant. Differences in repeated measures were analyzed by repeated-measures analysis of variance. Results: Plasma S1P levels decreased significantly from the baseline to low-salt diet period and increased from the low-salt to high-salt diet period. We observed this response in both salt-sensitive and salt-resistant individuals. Plasma S1P levels positively correlated with 24-hour urinary sodium excretion, but not 24-hour urinary potassium excretion. In line with plasma S1P level responses to salt intervention, systolic blood pressure (SBP) and mean arterial pressure (MAP) decreased from the baseline to low-salt diet period and increased from the low-salt to high-salt period. SBP positively correlated with plasma S1P and the correlation was stronger in salt-sensitive individuals than that in salt-resistant individuals. Conclusion: Low-salt dietary intervention decreases plasma S1P levels, whereas high-salt intervention reverses this change and S1P levels positively correlated with SBP in Chinese adults. This provides a high-efficiency and low-cost intervention for plasma S1P levels modulation, with implications for salt-induced blood pressure modulation. Trial registration: NCT02915315. Registered 27 September 2016, http://www.clinicaltrials.gov
Improper acupuncture causing severe multiple soft tissue damage:a case report
Authors: xie yulong , peng yan , zhou wenzong , Qin Qiantong , Wang Hui , Ni Bing , Wang Wenjie , Li Tianyu , Dong Yunxia , Miao Rui , Liu Yanqing , Jiang Donghong 
Published: November 2020

Abstract
Background Acupuncture therapy is well known. But there is no report that acupuncture in patients with coagulation dysfunction in stroke may lead to severe soft tissue injury. we report a case of acupuncture leading to severe soft tissue injury.Methods A 63-year-old female patient was hospitalized for stroke rehabilitation. After 2 years of discharge, severe subcutaneous hematoma caused by acupuncture was readmitted to hospital. She received medication and physical rehabilitation. we monitored the international standardized ratio (INR) in plasma and observed the degree of dissipation of subcutaneous hematoma. the changes of patients during treatment were explored by data analysis.Findings Subcutaneous hematoma (area 20×20 cm) disappeared completely after 3 weeks of physical factor therapy. Small range (area ≤3×3 cm) subcutaneous hematoma can heal itself within 2 weeks. Improper acupuncture is only one of the causes of soft tissue injury. Stroke with coagulation dysfunction is not a contraindication of acupuncture treatment.Interpretation Our report suggests that previously unanticipated acupuncture treatment for stroke patients with coagulation dysfunction may lead to severe multiple soft tissue injuries. Although coagulation dysfunction is not a contraindication of acupuncture treatment, acupuncture treatment for stroke patients with coagulation dysfunction should be vigilant, strengthen the level of acupuncture operation, and fundamentally eliminate the problem. These cases are validation and supplement of adverse clinical reactions caused by improper acupuncture.
Clinical Application and Evaluation of a New Combined Hip Prosthesis System: A Multicenter Randomized Controlled Non-inferiority Study
Authors: Luo Pan , Li Tengqi , Sun Wei , Li Zirong , Gao Fuqiang , Shi Lijun 
Published: November 2020

Abstract
Background: To evaluate the efficacy and safety of a new hip prosthetic system compared with the control group.Methods: A total of 140 patients (140 hips) who met the inclusion criteria in three clinical institutions were selected. The main outcome were to judge the excellent and good rate of Harris score in the experimental group and the control group 5 years after operation, and whether the lower limit of the 95% confidence interval of the difference between the two groups was greater than the non-inferior threshold (the non-inferior cutoff value was-10%). The secondary outcome were the comparison of Harris scores, the number of adverse events, safety test and evaluation, the results of X-ray films and the concise health status questionnaire (SF-36) between the two groups before operation and after operation.Results: During the 5-year follow-up, the excellent and good rates of Harris scores in the experimental group and the control group were 96.8% and 96.7% respectively, and the rate difference was 0.1%. The lower limit of the 95% confidence interval was -8.9%, which was greater than the non-inferiority boundary value (the non-inferiority boundary value was-10%). There was no significant difference in Harris score, adverse events, safety test, imaging, and SF-36 analysis between the two groups before operation and after operation.Conclusion: The new combined hip prosthesis system meets the requirements of clinical safety and effectiveness, and is worth popularizing in clinical application.Trial registration: researchregistry, researchregistry5771. Registered 1 July 2020 - Retrospectively registered. https://www.researchregistry.com/ researchregistry5771
Mas Receptor Activation Attenuates Allergic Airway Inflammation via Inhibiting JNK/CCL2-induced Macrophage Recruitment
Authors: Hong Luna , Chen Ming , Shi Jianting , Wang Qiujie , Guo Yimin , Liu Shanying , Pan Ruijian , Yuan Xiaoqing , Jiang Shanping 
Published: November 2020

Abstract
Background: Defective absorption of acute allergic airway inflammation is involved in the initiation and development of chronic asthma. After allergen exposure, there is a rapid recruitment of macrophages around the airways, which promote acute inflammatory responses. The Ang-(1-7)/Mas receptor axis reportedly plays protective roles in various tissue inflammation and remodeling processes in vivo. However, the exact role of Mas receptor and their underlying mechanisms during the pathology of acute allergic airway inflammation remains unclear. Methods: Mas receptor expression was assessed in ovalbumin-induced acute asthmatic murine model. Then we estimated the anti-inflammatory role of Mas receptor in vivo and explored expressions of several known inflammatory cytokines as well as phosphorylation levels of MAPK pathways. Mas receptor functions and underlying mechanisms were studied further in the human bronchial epithelial cell line (16HBE).Results: Mas receptor expression decreased in acute allergic airway inflammation. Multiplex immunofluorescence co-localized Mas receptor and EpCAM, indicated that Mas receptor may function in the bronchial epithelium. Activating Mas receptor through AVE0991 significantly alleviated macrophage infiltration in airway inflammation, accompanied with down-regulation of CCL2 and phosphorylation levels of MAPK pathways. Further studies in 16HBE showed that AVE0991 pre-treatment inhibited LPS-induced or anisomycin-induced CCL2 increase and THP-1 macrophages migration via JNK pathways.Conclusion: Our findings suggested that Mas receptor activation significantly attenuated CCL2 dependent macrophage recruitments in acute allergic airway inflammation through JNK pathways, which indicated that Mas receptor, CCL2 and phospho-JNK could be potential targets against allergic airway inflammation.
Transmittance of Tapered Photonic Crystal Fibers With Absorbing Coatings
Authors: Sicacha Mauricio Salazar , Minkovich Vladimir (or Uladzimir) Petrovich , Sotsky Alexander B. , Shilov Artur V. , Sotskaya Luidmila I. 
Published: November 2020

Abstract
The interaction effect of the fundamental mode of a photonic crystal fiber (PCF) with a thin-film absorbing coating deposited on a surface of a fiber cladding on the optical transmission of the PCF is studied. It is shown that the transmission has a quasi-periodic spectrum that is determined either by a resonance coupling between the leaky fundamental PCF mode and leaky modes of the coating, or between the leaky fundamental PCF mode and cladding modes localized between PCF air channels and the coating. Examples are presented of using this effect for fiber-optic sensors of refractive index, pressure, and for sensing an adsorption layer of ammonia molecules deposited on a coating surface contacting with air.
Impact of Socioeconomic Status on Prostate Cancer Outcomes Globally: A Protocol for Systematic Review and Meta-analysis
Authors: Nna Emmanuel Okechukwu , Ezeonwu Bertilla Uzoma , Obu Dorathy Chinwe , Khalid Abdullahi , Asiegbu Uzoma Vivian , Kenneth Kyegwim Nianglong , Ikeagwulonu Richard Chinaza , Onwuchekwa Chinemerem Eunice , Garba Maria Ahuoiza , Akuse Rosamund , Ayede Adejumoke Idowu , Udigwe Ifeoma Bridget , Afoke Kokogho , Okoye Ifeoma Joy 
Published: November 2020

Abstract
BackgroundOne in every four men will be affected by prostate cancer. Choice of treatment depends on factors including grade and stage of the disease, age of the patient, availability of treatment options and socioeconomic status. We aimed to develop a protocol to assess the impact of socioeconomic status on prostate cancer outcomes globally.Methods A search strategy is developed using MeSH, text words, and entry terms. Nine databases will be searched, including PubMed, African Journals Online (AJOL), Google Scholar, Scopus, Cochrane Library, CINAHL, Web of Science, Embase and ResearchGate.Only observational studies, retrievable in the English language will be included. The primary outcome of this study is the socioeconomic status of prostate cancer patients. Secondary outcomes include mortality due to prostate cancer, health related quality of life, prostate cancer recurrence, need for secondary treatment, time to return to work, treatment choice regret and hospice enrollment. Identified studies will be screened and selected based on inclusion criteria. Data items will be managed in Zotero software, Microsoft Excel and CMA software. Both quality scores and the risk of bias for individual studies will be reported. Studies will be assessed for methodological, clinical, and statistical heterogeneity. Funnel Plots will be used for assessing publication bias. DiscussionThis protocol will enable a transparent, reliable and accurate method for assessing the impact of socioeconomic status on the global prostate cancer outcomes. It will allow discussions on outcomes such as mortality due to prostate cancer and how income disparity and availability of treatment options can influence prostate cancer outcomes. The final report of this study will be published in a peer-reviewed journal and the findings will be made available to health authorities.Systematic review RegistrationThis protocol has been registered in PROSPERO, with registration number CRD42020213700
Current Developments in Delivering Customized Care: A Scoping Review
Authors: Minvielle Etienne , Fourcade Aude , Ricketts Tom , waelli Mathias 
Published: November 2020

Abstract
Background In recent years, there has been a growing interest in healthcare personalization and customization (e.g. personalized medicine and patient-centered care). While some positive impacts of these approaches have been reported, there has been a dearth of research on how these approaches are implemented and combined for healthcare delivery systems. Objective The present study undertakes a scoping review of current developments for delivering customized care, according to theoretical and practical guidelines for customization delivery approaches. Methods Article searches were initially conducted in November 2018, and updated in January 2019 and March 2019, according to Prisma guidelines. Two investigators independently searched MEDLINE, PubMed, PyscINFO, Web of Science, Science Direct and JSTOR, The search was focused on articles that included “care customization”, “personalized service and healthcare”, individualized care” and “targeting population” in the title or abstract. Inclusion and exclusion criteria were defined. Disagreements on study selection and data extraction were resolved by consensus and discussion between two reviewers. Results We identified 70 articles published between 2008 and 2019. Most of the articles (n = 43) were published from 2016 to 2019. Four categories of patient characteristics used for segmentation analysis emerged: clinical, psychosocial, service and costs. We observed they often coexisted with the most commonly described combinations, namely clinical, psychosocial and service. A minority of articles (n = 18) reported assessments on quality of care, experiences and costs. Finally, few articles (n= 6) formally defined a conceptual basis related to mass customization, whereas only half of articles used existing theories to guide their analysis or interpretation. Conclusions There is no common theory based strategy for providing customized care. In response, we have highlighted three areas for researchers and managers to advance the customized development concept in healthcare delivery systems: better define the content of the segmentation analysis and the intervention steps, demonstrate its added value, in particular its economic viability, and align the logics of action that underpin current efforts of customization. It would allow them to use customization to reduce costs and improve quality of care.