bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mediator complex subunit Med19 binds directly GATA DNA-binding zinc finger and functions with Med1 in GATA-driven gene regulationin vivo
Authors: Immarigeon Clément , Bernat-Fabre Sandra , Guillou Emmanuelle , Verger Alexis , Prince Elodie , Benmedjahed Mohamed A. , Payet Adeline , Couralet Marie , Monte Didier , Villeret Vincent , Bourbon Henri-Marc , Boube Muriel 
Published: April 2020

AbstractThe evolutionarily-conserved multiprotein Mediator complex (MED) serves as an interface between DNA-bound transcription factors (TFs) and the RNA Polymerase II machinery. It has been proposed that each TF interacts with a dedicated MED subunit to induce specific transcriptional responses. However, binary MED subunit - TF partnerships are probably oversimplified models. UsingDrosophilaTFs of the GATA family - Pannier (Pnr) and Serpent (Srp) - as a model, we have previously established GATA cofactor evolutionarily-conserved function for the Med1 Mediator subunit. Here, we show that another subunit, Med19, is required for GATA-dependent gene expression and interacts physically with Pnr and Srpin cellulo, in vivoandin vitrothrough their conserved C-zinc finger (ZF), indicating general GATA co-activator functions. Interestingly, Med19 is critical for the regulation of all tested GATA target genes which is not the case for Med1, suggesting differential use of MED subunits by GATAs depending on the target gene. Lastly, despite their presumed distant position within the MED middle module, both subunits interact physically. In conclusion, our data shed new light first on the MED complex, engaging several subunits to mediate TF-driven transcriptional responses and second, on GATA TFs, showing that ZF DNA-binding domain also serves for transactivation.
POMK regulates dystroglycan function via LARGE-mediated elongation of matriglycan
Authors: Walimbe Ameya S. , Okuma Hidehiko , Joseph Soumya , Yang Tiandi , Yonekawa Takahiro , Hord Jeffrey M. , Venzke David , Anderson Mary E. , Torelli Silvia , Manzur Adnan , Devereaux Megan , Cuellar Marco , Prouty Sally , Landa Saul Ocampo , Yu Liping , Xiao Junyu , Dixon Jack E. , Muntoni Francesco , Campbell Kevin P. 
Published: April 2020

AbstractMatriglycan [-GlcA-β1,3-Xyl-α1,3-]nserves as a scaffold in many tissues for extracellular matrix proteins containing laminin-G domains including laminin, agrin, and perlecan. Like-acetylglucosaminyltransferase-1 (LARGE) synthesizes and extends matriglycan on α-dystroglycan (α-DG) during skeletal muscle differentiation and regeneration; however, the mechanisms which regulate matriglycan elongation are unknown. Here, we show thatProtein O-Mannose Kinase (POMK), which phosphorylates mannose of core M3 (GalNac-β1,3-GlcNac-β1,4-Man) preceding matriglycan synthesis, is required for LARGE-mediated generation of full-length matriglycan on α-DG (∼150 kDa). In the absence ofPOMK, LARGE synthesizes a very short matriglycan resulting in a ∼90 kDa α-DG in mouse skeletal muscle which binds laminin but cannot prevent eccentric contraction-induced force loss or muscle pathology. Solution NMR spectroscopy studies demonstrate that LARGE directly interacts with core M3 and binds preferentially to the phosphorylated form. Collectively, our study demonstrates that phosphorylation of core M3 byPOMKenables LARGE to elongate matriglycan on α-DG, thereby preventing muscular dystrophy.
Defining an age cut-off for older offenders: a systematic review of literature
Authors: Merkt Helene , Haesen Sophie , Meyer Leila , Kressig Reto W. , Elger Bernice S. , Wangmo Tenzin 
Published: April 2020

PurposeIn the literature, 65 years is commonly used as the age to designate an older person in the community. When studying older prisoners, there is much variation. The purpose of this paper is to investigate how researchers define older offenders and for what reasons.Design/methodology/approachThe authors reviewed articles on health and well-being of older offenders to assess terminology used to describe this age group, the chosen age cut-offs distinguishing younger offenders from older offenders, the arguments provided to support this choice as well as the empirical base cited in this context.FindingsThe findings show that the age cut-off of 50 years and the term “older” were most frequently used by researchers in the field. The authors find eight main arguments given to underscore the use of specific age cut-offs delineating older offenders. They outline the reasoning provided for each argument and evaluate it for its use to define older offenders.Originality/valueWith this review, it is hoped to stimulate the much-needed discussion advancing towards a uniform definition of the older offender. Such a uniform definition would make future research more comparable and ensure that there is no ambiguity when researchers state that the study population is “older offenders”.
Learning environment, students' attitude and intention to enhance current knowledge in the context of choosing accounting career
Authors: Hatane Saarce Elsye , Setiono Felicia Jesslyn , Setiawan Fannie Felita , Semuel Hatane , Mangoting Yenni 
Published: April 2020

PurposeThis research examines the influence of the learning environment and students' attitudes towards choosing an accounting career mediated by intention to enhance the current knowledge.Design/methodology/approachThe online survey is adopted to test the research model of this research. There are 503 useable responses collected with the effective response rate of 72 per cent. Data analysis and hypothesis testing use Partial Least Square as part of the Structural Equation Modelling technique.FindingsThe results of this research indicate that accounting students possess positive attitude both towards the intention to enhance the current knowledge and choosing their accounting career. The learning environment includes educators and friends who give significant influence on students' intention. Besides, current knowledge enhancement is also discovered to be able to mediate the link between attitude, learning environment and intention to choose accounting career.Research limitations/implicationsDifferent generations may generate either different perception or different orientation in choosing accounting career. Therefore, future research can consider wider coverage and more updated object.Practical implicationsFindings of this research suggest that periodic improvement and renewal are necessary to administer to create an optimum learning environment, in term of teachers' capacity, teaching materials and supporting social environment.Originality/valueThis research contributes to any research related to attitudes towards choosing an accounting career. This study is the leading study that combines students' attitudes, learning environment, current knowledge enhancement, and career choice in one single model.
Does emotional labor color service actions in customer buying?
Authors: Seger-Guttmann Tali , Medler-Liraz Hana 
Published: April 2020

PurposeService research has highlighted the role of emotional labor in service delivery but has neglected service employees’ actions. This study aims to distinguish between the recurrent in-role and extra-role actions of service employees and to examine the joint effect of service employees’ actions and their emotional labor, which may color these actions on customer buying behavior (number of purchased items and total bill).Design/methodology/approachPhase I comprised two studies: Study 1 examined 70 service interaction videos to identify employees’ service actions, and Study 2 quantitatively validated the most frequent employee actions, used for further study, by examining 40 employee–customer interactions in fashion stores. For Phase II, Study 3 derived data from 60 service employees’ diaries to predict the joint effect of performed emotional labor and service actions on customer buying behavior.FindingsFindings revealed that emotional labor moderated the relationship between service actions and customer buying behavior. The relationship between in-role/extra-role actions and buying behavior was stronger in the lower surface-acting (less emotional faking) condition, whereas the relationship between in-role/extra-role actions and buying behavior was stronger for the higher deep-acting (more emotionally authentic) condition.Practical implicationsService organizations should not limit training to the more easily attained service actions. This possibility may be lacking if it ignores the emotional component that accompanied the action. This may shift the focus from customer satisfaction to customer delight.Originality/valueThis study is a pioneering effort to examine the specific circumstances in which service employees’ actions (regardless of in-role or extra-role status) will not produce the desired customer-related outcome in the presence of emotional labor.
Modeling and solving a five-echelon location–inventory–routing problem for red meat supply chain
Authors: Rahbari Misagh , Razavi Hajiagha Seyed Hossein , Raeei Dehaghi Mahdi , Moallem Mahmoud , Riahi Dorcheh Farshid 
Published: April 2020

Purpose
In this paper, multi-period location–inventory–routing problem (LIRP) considering different vehicles with various capacities has been investigated for the supply chain of red meat. The purpose of this paper is to reduce variable and fixed costs of transportation and production, holding costs of red meat, costs of meeting livestock needs and refrigerator rents.

Design/methodology/approach
The considered supply chain network includes five echelons. Demand considered for each customer is approximated as deterministic using historical data. The modeling is performed on a real case. The presented model is a linear mixed-integer programming model. The considered model is solved using general algebraic modeling system (GAMS) software for data set of the real case.

Findings
A real-world case is solved using the proposed method. The obtained results have shown a reduction of 4.20 per cent in final price of red meat. Also, it was observed that if the time periods changed from month to week, the final cost of meat per kilogram would increase by 43.26 per cent.

Originality/value
This paper presents a five-echelon LIRP for the meat supply chain in which vehicles are considered heterogeneous. To evaluate the capability of the presented model, a real case is solved in Iran and its results are compared with the real conditions of a firm, and the rate of improvement is presented. Finally, the impact of the changed time period on the results of the solution is examined.
A study on the process and mechanism of social enterprise’s legitimation
Authors: Xu Siqi , Xi Youmin 
Published: April 2020

PurposeThis paper aims to explore the complete process and underlying mechanism that social enterprises obtain legitimacy during interactions with stakeholders from theoretical integration of institutional theory and organization ecology perspective.Design/methodology/approachBased on theoretical classification, this paper selects six typical Chinese social enterprises and conducts a multi-case analysis.FindingsThe study finds that social enterprises aim at legitimizing single entity or industry and shaping stakeholders’ cognitive boundary simultaneously. Therefore, by adopting constrained cooperation and competition activities, social enterprises use normative isomorphism to achieve personal legitimation and combining ecological niche construction, social enterprises achieve organizational legitimation. By adopting fragmented cooperation-dominant or competition-dominant activities, social enterprises use mimic isomorphism supplemented by competitive isomorphism or population structure creation to obtain industry legitimation. By adopting dynamically integrated coopetition activities, social enterprises use mimic isomorphism and reflexive isomorphism to reach field legitimation.Originality/valueThis paper proposes a mechanism model that the coopetition with stakeholders influences the legitimation process, identifies four stages of social enterprise’s legitimation process and the types of legitimacy obtained in each stage and fills the gap of Chinese indigenous social enterprise research.
Multiple trait dimensions mediate stress gradient effects on plant biomass allocation, with implications for coastal ecosystem services
Authors: De Battisti Davide , Fowler Mike S. , Jenkins Stuart R. , Skov Martin W. , Bouma Tjeerd J. , Neyland Penelope J. , Griffin John N. 
Published: April 2020

Abstract

The plant economic spectrum (PES) predicts a suite of correlated traits in a continuum from resource conservation to rapid resource acquisition. In addition to competing for resources, plants need to cope with other environmental stresses to persist and reproduce. Yet, it is unclear how multiple strategies (i.e. traits uncorrelated with the PES) affect plant biomass allocation, hindering our ability to connect environmental gradients to ecosystem services.

We examined intraspecific dimensionality of leaf and root traits in the salt marsh pioneer species Spartina anglica across salinity, redox and sand content gradients, and related them to above‐ground and below‐ground plant biomass—properties associated with wave attenuation and sediment stabilization in coastal marshes.

Through principal component analysis, we did not find support for a single PES trait dimension (strategy), but instead identified four trait dimensions: (a) leaf economic spectrum (LES, leaf analogue of PES); (b) fine roots‐rhizomes; (c) coarse roots; and (d) salt extrusion. Structural equation modelling showed a shift towards the conservative side of the LES under increasing salinity, while redox had a positive influence on the coarse roots dimension. In turn, these trait dimensions were strongly associated with above‐ground and below‐ground biomass (BLW biomass) allocation.

These results indicate that under high salinity, plants will adopt a conservative strategy and will invest more in BLW biomass. Yet, high sediment redox would still allow plants to invest in above‐ground biomass. Therefore, plants' trait‐mediated biomass allocation depends on the specific combination of abiotic factors experienced at the local scale.

Synthesis. Our study highlights the importance of considering multiple ecological strategies for understanding the effect of the environment on plants. Abiotic stresses can influence multiple trait strategy‐dimensions, with consequences for ecosystem functioning.
Detecting invertebrate ecosystem service providers in orchards: traditional methods versus barcoding of environmental DNA in soil
Authors: Todd Jacqui H. , Simpson Robert M. , Poulton Joanne , Barraclough Emma I. , Villsen Kurt , Brooks Amber , Richards Kate , Jones Dan 
Published: April 2020

Abstract
The objective of this study was to assess barcoding of environmental DNA as a method for monitoring invertebrate ecosystem service providers in soil samples.
We selected 26 invertebrate ecosystem service providers that occur in New Zealand kiwifruit or apple orchards and produced mitochondrial cytochrome c oxidase gene subunit I (cytochrome oxidase I) and/or 28S ribosomal DNA sequences for each. Specific barcode primers were designed for each invertebrate ecosystem service provider and tested, along with generic barcoding cytochrome oxidase I primers, for their ability to detect DNA from invertebrate ecosystem service providers that had been added to sterilized and unsterilized soil samples.
Although the specific primers accurately detected the invertebrate ecosystem service providers in more than 96% of the samples, the generic cytochrome oxidase I primers detected only 37% of the invertebrate ecosystem service providers added to the sterilized samples and 2.5% in the unsterilized samples.
In a field test, we compared metabarcoding with traditional invertebrate trapping methods to detect the invertebrate ecosystem service providers in 10 kiwifruit and 10 apple orchards. All invertebrate ecosystem service providers were collected in traps in at least one orchard, but very few were identified by metabarcoding of soil environmental DNA.
Although the specific primers can be used as a tool for monitoring invertebrate ecosystem service providers in soil samples, methodological improvements are needed before metabarcoding of soil environmental DNA can be used to monitor these taxa.
Transition of Patients with Opioid Use Disorder from Buprenorphine to Extended‐Release Naltrexone: A Randomized Clinical Trial Assessing Two Transition Regimens
Authors: Comer Sandra D. , Mannelli Paolo , Alam Danesh , Douaihy Antoine , Nangia Narinder , Akerman Sarah C. , Zavod Abigail , Silverman Bernard L. , Sullivan Maria A. 
Published: April 2020

Background and ObjectiveWhen patients seek to discontinue buprenorphine (BUP) treatment, monthly injectable extended‐release naltrexone (XR‐NTX) may help them avoid relapse. The efficacy of low ascending doses of oral NTX vs placebo for patients transitioning from BUP to XR‐NTX is evaluated in this study.MethodsIn a phase 3, hybrid residential/outpatient study, clinically stable participants with opioid use disorder (N = 101), receiving BUP for more than or equal to 3 months and seeking antagonist treatment, were randomized (1:1) to 7 residential days of descending doses of BUP and low ascending doses of oral NTX (NTX/BUP, n = 50) or placebo (PBO‐N/BUP, n = 51). Both groups received standing ancillary medications and psychoeducational counseling. Following negative naloxone challenge, participants received XR‐NTX (day 8). The primary endpoint was the proportion of participants who received and tolerated XR‐NTX.ResultsThere was no statistical difference between groups for participants receiving a first dose of XR‐NTX: 68.6% (NTX/BUP) vs 76.0% (PBO‐N/BUP; P = .407). The mean number of days with peak Clinical Opiate Withdrawal Scale (COWS) score less than or equal to 12 during the treatment period (days 1‐7) was similar for NTX/BUP and PBO‐N/BUP groups (5.8 vs 6.3; P = .511). Opioid withdrawal symptoms during XR‐NTX induction and post‐XR‐NTX observation period (days 8‐11) were mild and similar between groups (mean peak COWS score: NTX/BUP, 5.1 vs PBO‐N/BUP, 5.4; P = .464). Adverse events were mostly mild/moderate.Conclusions and Scientific SignificanceLow ascending doses of oral NTX did not increase induction rates onto XR‐NTX compared with placebo. The overall rate of successful induction across treatment groups supports a brief BUP taper with standing ancillary medications as a well‐tolerated approach for patients seeking transition from BUP to XR‐NTX. (Am J Addict 2020;00:00–00)
Characterization of amoxicillin and clavulanic acid specific T‐cell clones from patients with immediate drug hypersensitivity
Authors: Ariza Adriana , Fernández‐Santamaría Rubén , Meng Xiaoli , Salas María , Ogese Monday O. , Tailor Arun , Bogas Gádor , Torres María José , Naisbitt Dean J. 
Published: April 2020

AbstractBackgroundBetalactam (BL) antibiotics are the most common cause of drug hypersensitivity. Amoxicillin (AX), which is often prescribed alongside clavulanic acid (Clav), is the most common elicitor. The aim of this study was to determine whether AX and Clav‐responsive T‐cells are detectable in patients with immediate hypersensitivity to AX‐Clav, to assess whether these T‐cells display the same specificity as that detected in skin and provocation testing, and to explore T‐cell activation pathways.MethodsDrug‐specific T‐cell clones were generated from immediate hypersensitive patients´ blood by serial dilution and repetitive mitogen stimulation. Antigen specificity was assessed by measurement of proliferation and cytokine release. CD4+/CD8+ phenotype and chemokine receptor expression were analyzed by flow cytometry.Results110 AX‐specific and 96 Clav‐specific T‐cell clones were generated from seven patients with positive skin test to either AX or Clav. Proliferation of AX‐ and Clav‐specific clones was dose‐dependent, and no cross‐reactivity was observed. AX‐ and Clav‐specific clones required antigen‐presenting cells to proliferate, and drugs were presented to CD4+ and CD8+ T‐cells by MHC class‐II and I, respectively. A higher secretion of IL‐13 and IL‐5 was detected in presence of the culprit drug compared with the alternative drug. Clones expressed CD69, CCR4, CXCR3, and CCR10.ConclusionsOur study details the antigen specificity and phenotype of T‐cell clones generated from patients with AX‐Clav‐induced immediate hypersensitivity diagnosed by positive skin test. AX‐ and Clav‐specific clones were generated from patients irrespective of whether AX or Clav was the culprit, although differences in cytokine secretion were observed.
The usefulness of MRI Classification System (MRICS) in a cerebral palsy cohort
Authors: Nagy Eszter , Herbert Zsuzsanna , Péter István , Csorba Eszter , Skobrák Andrea , Farkas Nelli , Hollódy Katalin 
Published: April 2020

AbstractAimOur aim was to investigate perinatal and clinical factors associated with children with cerebral palsy (CP) using magnetic resonance imaging (MRI). The distribution of MRI patterns was based on the MRI classification system (MRICS). Associations between perinatal/clinical characteristics and MRI patterns were also investigated.MethodsA population‐based cohort study was performed; those 257 children (58.0% male) were enrolled from our CP database who born between 1990 and 2015 in Southwest Hungary and had at least one MRI scan.ResultsBrain maldevelopments were found in 18.7% of our patients, 83.7% of those born at term. Grey matter lesions were found in 19.8% of our patients, and 80.0% of those children were born at term. The rate of white matter injuries was the highest (35.4%); 69.0% of these patients were born before 37th week of gestation. MRI revealed no abnormalities in 13.6% of children with CP. The best values of gross/fine motor and cognitive function tests were found in children with normal MRI and with grey matter injuries. The prevalence of epilepsy was above 60% in every group with an abnormal MRI.ConclusionMRI results were conclusive in 86.4% of children with CP. It is highly encouraged to perform cranial MRI in every patient with CP.
Case contamination in electronic health records based case‐control studies
Authors: Wang Lu , Schnall Jill , Small Aeron , Hubbard Rebecca A. , Moore Jason H. , Damrauer Scott M. , Chen Jinbo 
Published: April 2020

AbstractClinically relevant information from electronic health records (EHRs) permits derivation of a rich collection of phenotypes. Unlike traditionally designed studies where scientific hypotheses are specified a priori before data collection, the true phenotype status of any given individual in EHR‐based studies is not directly available. Structured and unstructured data elements need to be queried through preconstructed rules to identify case and control groups. A sufficient number of controls can usually be identified with high accuracy by making the selection criteria stringent. But more relaxed criteria are often necessary for more thorough identification of cases to ensure achievable statistical power. The resulting pool of candidate cases consists of genuine cases contaminated with noncase patients who do not satisfy the control definition. The presence of patients who are neither true cases nor controls among the identified cases is a unique challenge in EHR‐based case‐control studies. Ignoring case contamination would lead to biased estimation of odds ratio association parameters. We propose an estimating equation approach to bias correction, study its large sample property, and evaluate its performance through extensive simulation studies and an application to a pilot study of aortic stenosis in the Penn medicine EHR. Our method holds the promise of facilitating more efficient EHR studies by accommodating enlarged albeit contaminated case pools.