bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic value of serum miR-145 and miR-185 as targeting of the APRIL oncogene in the B-cell chronic lymphocytic leukemia
Authors: Bagheri Malihe , Khansarinejad Behzad , Mosayebi Ghasem , Moradabadi Alireza , Mondanizadeh Mahdieh 
Published: March 2020

Abstract
Background: Chronic lymphocytic leukemia (CLL) is one of the most common hematologic malignancy in adults worldwide. This cancer has a poor prognosis at different stages. So, the identification of new biomarkers is important for early diagnosis of B-CLL. Considering the oncogenic role of APRIL molecule in this leukemia as well as the regulatory role of microRNAs (miRNAs) in different signaling pathways, the present study evaluated the miRNAs targeting APRIL gene in B-CLL.Methods: The miRNAs were predicted and selected using bioinformatics algorithms. A total of 80 plasma samples (40 samples of healthy individuals and 40 samples of B-CLL patients) were subjected to RNA extraction and synthesis of cDNA. The expressions levels of predicted miRNAs and APRIL gene in plasma of B-CLL patients and healthy individuals were assessed by Real time PCR analysis. ROC analysis was performed to investigate the role predicted miRNAs as novel biomarkers in diagnosis of B-CLL. Results: The results of the prediction showed that miR-145-5p and miR-185-5p target the APRIL gene. The expression level of APRIL gene was strikingly higher in plasma of B-CLL patients than in the healthy individuals (102, P= 0.001). On the other hand, expression levels of miR-145-5p and miR-185-5p were strikingly lower in B-CLL patients than in the healthy individuals (0.07, P= 0.001) (0.29, P= 0.001). Also, ROC curve analyses demonstrated that miR-145-5p and miR-185-5p are specific and sensitive and may serve as new biomarkers for the early detection of B-CLL. Conclusions: These data suggest that the study miRNAs may have a role in B-CLL development and progression. Moreover, miR-145-5p and miR-185-5p can be served as a novel and potential biomarker in the diagnosis of B-CLL.
A nomogram based on Aspartate Aminotransferase/Alanine Aminotransferase (AST/ALT) ratio after surgery to predict the prognostic value in patients with gastric cancer
Authors: Li Linfang , Xing Shan , Xue Ning , Wu Miantao , Liang Yaqing , Xu Qingxia , Feng Lingmin , Zeng Qiuyao , Chen Shulin 
Published: March 2020

Abstract
Background This study aimed to develop an effective nomogram for predicting overall survival (OS) in surgically treated gastric cancer. Methods We retrospectively evaluated 190 gastric cancer in this study. Cox regression analyses were performed to identify significant prognostic factors for OS in patients with resectable gastric cancer. The predictive accuracy of nomogram was assessed by calibration plot, concordance index (C-index) and decision curve, and then were compared with the traditional TNM staging system. Based on the total points (TPS) by nomogram, we further divided patients into different risk groups. Results On multivariate analysis of the 190 cohort, independent factors for survival were age, clinical stage and Aspartate Aminotransferase/Alanine Aminotransferase (SLR), which were entered into the nomogram. The calibration curve for the probability of OS showed that the nomogram-based predictions were in good agreement with actual observations. And the C-index of the established nomogram for predicting OS had a superior discrimination power compared with the TNM staging system [0.768 (95% CI: 0.725-0.810) vs 0.730 (95% CI: 0.688-0.772), p < 0.05]. Decision curve also demonstrated that the nomogram was better than TNM staging system. Based on the TPS of the nomogram, we further subdivided the study cohort into 3 groups: low risk (TPS ≤ 158), middle risk (158 < TPS ≤ 188), high risk (TPS > 188), the differences of OS rate were significant in the groups. Conclusions The established nomogram resulted in more accurate prognostic prediction for individual patient with resectable gastric cancer.
The Mid-term outcomes of ankle Dias-Tachdjian PEER physeal fracture in children treat by ORIF with One-stage medial-lateral combined incision
Authors: XIANG Feng , LI Bo , XIAO Sheng , Wen Jie , LIU Hong , LI Xin , FANG Ke , TANG Zhongwen , CAO Shu , Lee Bo , LI Fanling , ZHU Kewei 
Published: March 2020

Abstract
Objectives: To report the mid-term outcomes and complications of ankle Dias-Tachdjian pronation-eversion external rotation (PEER) physeal fracture in children treat by ORIF with combined medial and lateral incision. Materials and Methods: A total of 21 children (12 male and 9 female patients with ankle Dias-Tachdjian PEER physeal fracture, underwent open reduction treatment with combined medial and lateral incision between Jan, 2015 and Oct, 2017. The surgical outcomes were assessed by X-ray film after operation. The lateral distal tibia angle (LDTA) was measured to evaluate angular deformity and the X rays was taken to evaluate the premature physeal arrest. Result: All the patients were followed up for an average time of 20.1 months (ranging from 17 to 25 months). Bone healing was achieved in all cases. American Orthopedic Foot and Ankle Society-Ankle and Hindfoot score(AOFAS-AH) of the patients improved from median 31(11,38) before operation to median 68(63,73) post-operation, median score 91(87,96) at last follow-up. Lateral distal tibia angle (LDTA) of the patients improved from 70.5±4.9 before operation to 90.0±1.2 post-operation, measure 90.6±3.7 at last follow-up. The differences between pre and post operation and the differences between pre-operation and last follow-up in both findings were statistically significant (P<0.05). There is no premature physeal closure, ankle deformity in 19 cases. They could normally exercise and take part in normal sport activities. The remaining 2 cases had physeal bone bridge and premature physeal closure but could still carry on daily activities and thus were categorized as good. Conclusion: ORIF with one-stage medial-lateral combined incision for ankle Dias-Tachdjian PEER physeal fracture in children can decrease the rate of premature physeal closure and has excellent efficacy for fracture union and the recovery of ankle functions.
Adolescents Nutrition Knowledge: A mixed methods Research.
Authors: Mafugu Tafirenyika 
Published: March 2020

Abstract
Background Poor nutrition has been associated with lack of nutrition education, which leads to chronic diseases like diabetes, hypertension, obesity and coronary artery diseases. The objectives of the study were to evaluate learners’ knowledge of nutrition education principles and to determine how the knowledge of nutrition principles could be improved among learners. Methods Mixed methods cross-sectional research with application of semi-structured face-to-face interviews and a structured questionnaire were used to collect data. Eight stake-holders participated in the qualitative component of the study while 108 learners from 8 different schools responded to the questionnaire. The qualitative data was analyzed by coding and categorizing the data into themes and sub-themes while the quantitative data was analyzed using SPSS yielding frequencies, percentages, means and standard deviations. Results A significant proportion of learners lacked the nutrition knowledge due to the absence of health promotion campaigns in the schools and teaching of the nutrition principles was only limited to Life Science in the high school curriculum. Majority (56%) of the learners were taught nutrition education at primary school mainly in the Life Orientation curriculum while less than 2% were taught in grade 11 and 12. About 92% of the learners were not aware of the nutritional value of fruits, vegetables, milk and fats. Conclusions Incorporation of nutrition education in a compulsory subject in high school and in-school nutrition education sessions in school gatherings that involve various stakeholders is critical in improving the nutrition knowledge of stakeholders. Such interventions will assist in curbing diet related chronic diseases.
Hsa_circ_0005273 regulates YAP1-Hippo signaling pathway by targeting miR-200a-3p to promote breast cancer progression.
Authors: Wang Xuehui , Ji Changle , Luo Qifeng , Hu Jiashu , Deng Xiaochong , Zheng Wenfang , Yu Yunhe , Hua Kaiyao , Zhou Xiqian , Fang Lin 
Published: March 2020

Abstract
Background : Hsa_circ_0005273 is a circRNA generated from several exons of PTK2. However, the potential functions of hsa_circ_0005273 in breast cancer remains unknown. Here we aim to explore the role of hsa_circ_0005273 in BC. Methods : We chose miR-200a-3p as the potential target of hsa_circ_0005273. The expression levels of hsa_circ_0005273 and miR-200a-3p were examined in BC tissues compared with adjacent normal tissues by qRT-PCR. To characterize the function of hsa_circ_0005273, experiments of cell proliferation and migration were performed in BC cell lines infected with lentivirus targeting hsa_circ_0005273. In addition, xenografts experiment in nude mice were performed to evaluate the effect of hsa_circ_0005273 in BC. Luciferase reporter assay was conducted to confirm the relationship between hsa_circ_0005273 and miR-200a-3p as well as miR-200a-3p andYAP1. Results : Hsa_circ_0005273 is over-expressed in BC tissues and cell lines, whereas miR-200a-3p expression is repressed. Depletion of has_circ_0005273 or upregulation of miR-200a-3p inhibited the proliferation and migration of BC cells in vitro and vivo. Mechanistically, hsa_circ_0005273 upregulated YAP1 by targeting miR-200a-3p and activated Hippo signaling pathway to promote BC progression. Conclusions : Hsa_circ_0005273 regulates the miR-200a-3p/YAP1 axis and activates Hippo signaling pathway to promote BC progression, and it may serve as a potential biomarker and therapeutic target. Keywords : breast cancer, hsa_circ_0005273, miR-200a-3p,YAP1, progression
Clinical Diagnostic Value for Colorectal cancer Based on Serum CEA, CA24-2 and CA19-9
Authors: Rao Hui , Wu Heming , Huang Qingyan , Yu Zhikang , Zhang Qunji , Zhong Zhixiong 
Published: March 2020

Abstract
Background To explore the clinical value of a combined detection of serum concentration of carcinoembryonic antigen (CEA), carbohydrate antigen 24-2 (CA24-2), and carbohydrate antigen 19-9 (CA19-9) for colorectal cancer (CRC). Methods The levels of serum tumor markers (CEA, CA24-2 and CA19-9) and clinicopathologic features in patients with colorectal cancer in Meizhou People's Hospital were evaluated. In addition, KRAS/NRAS, PIK3CA and BRAF mutations were detected in some patients with colorectal cancer. Results A total of 2,281 patients (1,420 males and 861 females) were recruited in the study, included 1,578 colorectal cancer patients and 703 controls. The levels of CEA, CA24-2 and CA19-9 in colorectal cancer group was significantly higher than control group. The sensitivities of three individual markers were lower than 30%, which individual sensitivity of the tumor markers sorted in descending order were CEA> CA19-9> CA24-2. The specificities of three individual markers were more than 92%, and the specificity sorted in descending order were CA24-2> CA19-9> CEA. The prediction equation excluding the risk of colorectal cancer was. Probability (normal) = Exp (-5.47 - 0.28CEA - 0.11 CA242 + 0.001 CA199)/ (1+ Exp (-5.47 - 0.28CEA - 0.11 CA242 + 0.001 CA199)). There were no significant differences in age, gender, histology type, differentiation, depth of invasion and TNM stage between mutations in KRAS/NRAS , BRAF and PIK3CA genes or not, respectively. Conclusions Serum CEA, ca24-2, and ca19-9 are valuable noninvasive indicators for the detection and screening of patients with early colon cancer. We need to look for other, more sensitive tumor markers.
Seed proteins mining for development, nutrition and germination using comparative proteomics analysis in quinoa（Chenopodium quinoa willd.)
Authors: Xu Suxia , Huang Qingyun , Li Ziyang , Huang Yijin , Lin Chunsong , He Enming , Lin Fucong 
Published: March 2020

Abstract
Background Recently quinoa( Chenopodium quinoa willd., 2n=4x=36)raises worldwide popularity with its totally nutrition, stress-tolerance, which leads quinoa to a strategic global food from an Andean native crop. However, seed pre-harvest sprouting, saponin contents e.c., restrict greatly quinoa production and popularity. In the study, we successfully used a combinational proteomics of TMT labeling and parallel reaction monitoring (PRM) to assess proteome changes relating to seed maturation conversion in quinoa to help accelerate genetic improvement for quinoa. Results In total, 6,097 proteins were identified and 4,770 proteins were quantified. Of them, 581 were differential expressed proteins (DEPs). Based on PRM data, seventeen DEPs were identified and quantified, including thirteen down-regulated proteins and four up-regulated proteins. Seventeen DEPs involved in oleanane-type saponins bio-synthesis (β-amyrin 28-oxidase), seed generation (β-Amylase), seed dormancy (late embryogenesis abundant proteins, Cyprosin), seed nutrition (globulin seed storage proteins), accumulation of sugars under seed desiccation (EARLY-RESPONSIVE TO DEHYDRATION 7 protein), pre-harvest sprouting (seed biotin-containing protein SBP65, ABA-inducible protein PHV A1), and enzymes related promoting seed maturation (bifunctional purple acid phosphatase 26), and pigment biosynthesis (3-O-glucosyltransferase). Conclusions We present the high-quality proteomics analysis of quinoa assessing proteome changes during seed maturation conversion. Our results summarize a valuable proteome profiles characterizing quinoa seed maturation. The DEPs are candidate for the functional analyses of proteins regulating seed maturation conversion in quinoa, which provide an important first step towards the genetic improvement of quinoa.
Influence of a 100-mile ultramarathon on the heart rate and the heart rate variability
Authors: Schrieber Simone , Paech Christian , Wüstenfeld Jan , Dähnert Ingo , Wolfarth Bernd , Thouet Thomas , Hellinger Paul Schmidt- 
Published: March 2020

Abstract

INTRODUCTION: The aim of this study was to investigate the impact of an ultra-marathon (UM) with a distance of 100 miles on heart rate (HR) and heart rate variability (HRV). METHODS: Altogether, 28 runners (25 men and 3 women) received a 24-hour long-term ECG one week before the UM (U1), immediately after (U2) and after a week of recovery (U3). The influence of age, body mass index (BMI), HR and HRV on the run time as well as recovery were investigated. RESULTS: A rise in the baseline heart rate accompanied by a significant drop in SDNN values ​​(the standard deviation of all normal RR intervals) was found. Except for the age of the runners, BMI, HF and HRV did not predict the competition time. Full return of HRV to the athlete’s individual baseline did not occur within one week. There were no significant differences between finishers and non-finishers in analyzed parameters. CONCLUSION: The present results show that a 100-mile run leads to an increase in sympathetic activity and thus to an increase in heart rate and a decrease in HRV. In addition, HRV seems to be a suitable parameter to evaluate full recovery after a 100-mile run.
Identification of Dietary Molecules as Therapeutic Agents to Combat COVID-19 Using Molecular Docking Studies
Authors: Khan Mohammad Faheem , Khan Mohsin Ali , Khan Zaw Ali , Ahamad Tanveer , Ansari Waseem Ahmad 
Published: March 2020

Abstract
Recently, a new and fatal strain of coronavirus named as SARS-CoV-2 (Disease: COVID-19) appeared in Wuhan, China in December of 2019. Due to its fast growing human to human transmission and confirmed cases in nearly every country, it has been declared as pandemic by World Health Organisation (WHO) on 11 March 2020. Till now, there is no therapy such as vaccines and specific therapeutic agents available globally. Inspite of this, some protease inhibitors and antiviral agents namely lopinavir, ritonavir, remdisivir and chloroquine are under investigation and also implemented in several countries as therapeutic agents for the treatment of COVID-19. Seeing the health crisis across the world, it was our aim to find out a suitable drug candidate which could target SARS-CoV-2. For this purpose, molecular docking of 7 proteinsof SARS-CoV-2 was done with 18active constituents that have previously been reported to be antiviral or anti-SARS-CoV agents. The docking results of these 18 compounds were compared with 2 FDA approved drugs that have are currently being used in COVID 19, namely Remdesivir and Chloroquine. Our result revealed that among all, epigallocatechin gallate (EGCG), a major constituent of green tea, is the lead compound that could fit well into the binding sites of docked proteins of SARS-CoV-2. EGCG showed very strong molecular interactions with binding energies -9.30, -8.66, -8.38, -7.57, -7.26, -6.99 and -4.90 kcal/mole for6y2e, 6vw1, 6vww, 6lxt,6vsb, 6lu7 and 6lvnproteins of SARS-CoV-2, respectively.Therefore, EGCG as per our results, should be explored as a drug candidate for the treatment of COVID-19.
Perspectives on Healthy Aging in Middle Age: a qualitative study
Authors: solhi Mahnaz , pirouzeh Razieh , Zanjari Nasibeh 
Published: March 2020

Abstract
Background: How to deal with the phenomenon of population aging is influenced by the way we on aging. Understanding how middle-aged people perceive healthy aging and what they need to do to stay healthy as they age can help public policy planning to enhance the lifestyles of middle-aged and elderly people. The purpose of this study was to clarify the concept of healthy aging and look at strategies to achieve it from the perspective of middle aged people in Tehran. Methods: This study was conducted on 21 Iranian middle-aged people aged 45-59 years old with a qualitative approach and content analysis method. Sampling was carried out gradually until data saturation through purposive sampling was achieved. Results: From the perspective of the participants, healthy aging is being physically healthy, having good mental health, financial welfare, and social support. From the perspective the subjects studied, financial planning for the future, promoting physical health, promoting psychological health, and maintaining and improving relationships are strategies needed to achieve healthy aging. Discussion: From the point of view of middle-aged people in the studied population healthy aging is affected by the economic status and the role of financial factors on health is specifically emphasized. The effort put in by middle-aged people to prepare for physical, mental, social, spiritual, and financial aspects in life can lead to a better experience when aging. In addition to personal preparation of middle-aged people for their future, given the importance of the financial aspect in Iranian society, support of the public sector is important to create a suitable environment for the aging population. Keywords: Health, Aging, Middle-Aged, Strategy
Caspofungin and LTX-315 inhibit SARS-CoV-2 replication by targeting the nsp12 polymerase
Authors: Wang Min , Ye Fei , Su Jiaqi , Zhao Jingru , Yuan Bin , Huang Baoying , Peng Qi , Peng Ruchao , Sun Yan , Bai Suran , Wang Xixi , Yang Wei , Fan Zheng , Wang Wenling , Wu Guizhen , Gao George F , Tan Wenjie , Shi Yi 
Published: March 2020

Abstract
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously designated as 2019-nCoV) outbreak has caused global concern1. Currently, there are no clinically approved specific drugs or vaccines available for this virus. The viral polymerase is a promising target for developing broad- spectrum antiviral drugs. Here, based on the highly similar structure of SARS- CoV non-structural protein 12 (nsp12) polymerase subunit2, we applied virtual screen for the available compounds, including both the FDA-approved and under- clinic drugs, to identify potential antiviral molecules against SARS-CoV-2. We found two drugs, the clinically approved anti-fungi drug Caspofungin Acetate (Cancidas) and the oncolytic peptide LTX-315, can bind SARS-CoV-2 nsp12 protein to block the polymerase activity in vitro. Further live virus assay revealed that both Caspofungin Acetate and LTX-315 can effectively inhibit SARS-CoV-2 replication in vero cells. These findings present promising drug candidates for treatment of related diseases and would also stimulate the development of pan- coronavirus antiviral agents.Authors Min Wang, Fei Ye, Jiaqi Su, Jingru Zhao, and Bin Yuan contributed equally to this work.
Food Fortification as a Preventive Factor of Micronutrient Deficiency
Authors: Mayurnikova Larisa , Koksharov Arkadiy , Krapiva Tatyana , Novoselov Sergey 
Published: March 2020

Introduction. The problem of micronutrient deficiency remains a relevant issue all over the world. However, it is mostly developed countries that practice food fortification. The FAO and the WHO accumulate related experience and summarize it in various documents. Yet some aspects of the problem can be solved on state level. The present research featured micronutrient deficit and preventive measures in several countries taking into account local food traditions. Since 1920, a number of industrially developed northern countries have started developing and implementing various food fortification programs. Similar programs are being introduced in East, Central, and South Africa and Southeast Asia. For 40 years, Russia has been taking various measures to prevent micronutrient deficiency and related diseases.
Results and discussion. The research revealed the social and economic measures of food fortification that were found lacking in these countries. The main problem proved to be iodine, iron, and vitamin A deficiency. However, lack of other micronutrients also remains a burning issue. The present paper gives an overview of iodine, selenium, and iron deficiencies in several countries. The authors proposed several solutions, e.g. food group selection, food additives (mono or premix), various ways of fortification, etc. The article also contains a list of main Russian regulatory documents that control the production and turnover of food fortification. The authors showed advantages of food fortification of mass consumption products, e.g. the low cost of processing, the affordability of enriched products, their availability for different social population, the well-developed regulation standards, the good social effect of food fortification projects, their economic efficiency, etc. Taking into consideration the environmental changes and various geopolitical and economic factors that negatively affect nutrition and population health, the authors recommend to continue evidence-based research in this direction to develop new technologies and food supplements.
Conclusion. The novelty of the research lies in the fact that it reveals priority areas for prospective scientific research in food fortification based on a thorough analysis of the existing experience. From the point of view of practical application, the research offers a deeper understanding of the problem, stresses its relevance, sets goals and objectives of future studies, and offers some ideas for preventive programs.