bluuebunny/crossref_metadata_2025_split huggingface dataset:
A mechanism of defect-enhanced phase transformation kinetics in lithium iron phosphate olivine
Authors: Hong Liang , Yang Kaiqi , Tang Ming 
Published: December 2019

AbstractAntisite defects are a type of point defect ubiquitously present in intercalation compounds for energy storage applications. While they are often considered a deleterious feature, here we elucidate a mechanism of antisite defects enhancing lithium intercalation kinetics in LiFePO4 by accelerating the FePO4 → LiFePO4 phase transformation. Although FeLi antisites block Li movement along the [010] migration channels in LiFePO4, phase-field modeling reveals that their ability to enhance Li diffusion in other directions significantly increases the active surface area for Li intercalation in the surface-reaction-limited kinetic regime, which results in order-of-magnitude improvement in the phase transformation rate compared to defect-free particles. Antisite defects also promote a more uniform reaction flux on (010) surface and prevent the formation of current hotspots under galvanostatic (dis)charging conditions. We analyze the scaling relation between the phase boundary speed, Li diffusivity and particle dimensions and derive the criteria for the co-optimization of defect content and particle geometry. A surprising prediction is that (100)-oriented LiFePO4 plates could potentially deliver better performance than (010)-oriented plates when the Li intercalation process is surface-reaction-limited. Our work suggests tailoring antisite defects as a general strategy to improve the rate performance of phase-changing battery compounds with strong diffusion anisotropy.
Pandemic influenza virus vaccines boost hemagglutinin stalk-specific antibody responses in primed adult and pediatric cohorts
Authors: Nachbagauer Raffael , Salaun Bruno , Stadlbauer Daniel , Behzadi Mohammad A. , Friel Damien , Rajabhathor Arvind , Choi Angela , Albrecht Randy A. , Debois Muriel , García-Sastre Adolfo , Rouxel Ronan N. , Sun Weina , Palese Peter , Mallett Corey P. , Innis Bruce L. , Krammer Florian , Claeys Carine 
Published: December 2019

AbstractLicensed influenza virus vaccines target the head domain of the hemagglutinin (HA) glycoprotein which undergoes constant antigenic drift. The highly conserved HA stalk domain is an attractive target to increase immunologic breadth required for universal influenza virus vaccines. We tested the hypothesis that immunization with a pandemic influenza virus vaccine boosts pre-existing anti-stalk antibodies. We used chimeric cH6/1, full length H2 and H18 HA antigens in an ELISA to measure anti-stalk antibodies in recipients participating in clinical trials of A/H1N1, A/H5N1 and A/H9N2 vaccines. The vaccines induced high titers of anti-H1 stalk antibodies in adults and children, with higher titers elicited by AS03-adjuvanted vaccines. We also observed cross-reactivity to H2 and H18 HAs. The A/H9N2 vaccine elicited plasmablast and memory B-cell responses. Post-vaccination serum from vaccinees protected mice against lethal challenge with cH6/1N5 and cH5/3N4 viruses. These findings support the concept of a chimeric HA stalk-based universal influenza virus vaccine. clinicaltrials.gov: NCT02415842.
Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration
Authors: da Silva Marcelo S. , Cayres-Silva Gustavo R. , Vitarelli Marcela O. , Marin Paula A. , Hiraiwa Priscila M. , Araújo Christiane B. , Scholl Bruno B. , Ávila Andrea R. , McCulloch Richard , Reis Marcelo S. , Elias Maria Carolina 
Published: December 2019

AbstractThe co-synthesis of DNA and RNA potentially generates conflicts between replication and transcription, which can lead to genomic instability. In trypanosomatids, eukaryotic parasites that perform polycistronic transcription, this phenomenon and its consequences are still little studied. Here, we showed that the number of constitutive origins mapped in the Trypanosoma brucei genome is less than the minimum required to complete replication within S-phase duration. By the development of a mechanistic model of DNA replication considering replication-transcription conflicts and using immunofluorescence assays and DNA combing approaches, we demonstrated that the activation of non-constitutive (backup) origins are indispensable for replication to be completed within S-phase period. Together, our findings suggest that transcription activity during S phase generates R-loops, which contributes to the emergence of DNA lesions, leading to the firing of backup origins that help maintain robustness in S-phase duration. The usage of this increased pool of origins, contributing to the maintenance of DNA replication, seems to be of paramount importance for the survival of this parasite that affects million people around the world.
Podocyte hypertrophic stress and detachment precedes hyperglycemia or albuminuria in a rat model of obesity and type2 diabetes-associated nephropathy
Authors: Minakawa Akihiro , Fukuda Akihiro , Sato Yuji , Kikuchi Masao , Kitamura Kazuo , Wiggins Roger C. , Fujimoto Shouichi 
Published: December 2019

AbstractType2 diabetes-associated nephropathy is the commonest cause of renal failure. Mechanisms responsible are controversial. Leptin-deficient hyperphagic Zucker (fa/fa) rats were modeled to test the hypothesis that glomerular enlargement drives podocyte hypertrophic stress leading to accelerated podocyte detachment, podocyte depletion, albuminuria and progression. By 6weeks, prior to development of either hyperglycemia or albuminuria, fa/fa rats were hyperinsulinemic with high urinary IGF1/2 excretion, gaining weight rapidly, and had 1.6-fold greater glomerular volume than controls (P < 0.01). At this time the podocyte number per glomerulus was not yet reduced although podocytes were already hypertrophically stressed as shown by high podocyte phosphor-ribosomal S6 (a marker of mTORC1 activation), high urinary pellet podocin:nephrin mRNA ratio and accelerated podocyte detachment (high urinary pellet podocin:aquaporin2 mRNA ratio). Subsequently, fa/fa rats became both hyperglycemic and albuminuric. 24 hr urine albumin excretion correlated highly with decreasing podocyte density (R2 = 0.86), as a consequence of both increasing glomerular volume (R2 = 0.70) and decreasing podocyte number (R2 = 0.63). Glomerular podocyte loss rate was quantitatively related to podocyte detachment rate measured by urine pellet mRNAs. Glomerulosclerosis occurred when podocyte density reached <50/106um3. Reducing food intake by 40% to slow growth reduced podocyte hypertrophic stress and “froze” all elements of the progression process in place, but had small effect on hyperglycemia. Glomerular enlargement caused by high growth factor milieu starting in pre-diabetic kidneys appears to be a primary driver of albuminuria in fa/fa rats and thereby an under-recognized target for progression prevention. Progression risk could be identified prior to onset of hyperglycemia or albuminuria, and monitored non-invasively by urinary pellet podocyte mRNA markers.
A Deep Learning Framework for Design and Analysis of Surgical Bioprosthetic Heart Valves
Authors: Balu Aditya , Nallagonda Sahiti , Xu Fei , Krishnamurthy Adarsh , Hsu Ming-Chen , Sarkar Soumik 
Published: December 2019

AbstractBioprosthetic heart valves (BHVs) are commonly used as heart valve replacements but they are prone to fatigue failure; estimating their remaining life directly from medical images is difficult. Analyzing the valve performance can provide better guidance for personalized valve design. However, such analyses are often computationally intensive. In this work, we introduce the concept of deep learning (DL) based finite element analysis (DLFEA) to learn the deformation biomechanics of bioprosthetic aortic valves directly from simulations. The proposed DL framework can eliminate the time-consuming biomechanics simulations, while predicting valve deformations with the same fidelity. We present statistical results that demonstrate the high performance of the DLFEA framework and the applicability of the framework to predict bioprosthetic aortic valve deformations. With further development, such a tool can provide fast decision support for designing surgical bioprosthetic aortic valves. Ultimately, this framework could be extended to other BHVs and improve patient care.
2-D Peripheral image quality metrics with different types of multifocal contact lenses
Authors: García García Miguel , Wahl Siegfried , Pusti Dibyendu , Artal Pablo , Ohlendorf Arne 
Published: December 2019

AbstractTo evaluate the impact of multifocal contact lens wear on the image quality metrics across the visual field in the context of eye growth and myopia control. Two-dimensional cross-correlation coefficients were estimated by comparing a reference image against the computed retinal images for every location. Retinal images were simulated based on the measured optical aberrations of the naked eye and a set of multifocal contact lenses (centre-near and centre-distance designs), and images were spatially filtered to match the resolution limit at each eccentricity. Value maps showing the reduction in the quality of the image through each optical condition were obtained by subtracting the optical image quality from the theoretical physiological limits. Results indicate that multifocal contact lenses degrade the image quality independently from their optical design, though this result depends on the type of analysis conducted. Analysis of the image quality across the visual field should not be oversimplified to a single number but split into regional and groups because it provides more insightful information and can avoid misinterpretation of the results. The decay of the image quality caused by the multifocal contacts alone, cannot explain the translation of peripheral defocus towards protection on myopia progression, and a different explanation needs to be found.
Association between high-fat diet feeding and male fertility in high reproductive performance mice
Authors: Gómez-Elías M. D. , Rainero Cáceres T. S. , Giaccagli M. M. , Guazzone V. A. , Dalton G. N. , De Siervi A. , Cuasnicú P. S. , Cohen D. J. , Da Ros V. G. 
Published: December 2019

AbstractThe increasing worldwide prevalence of metabolic syndrome (MetS), especially in younger populations, is a risk factor for fertility disorders. However, a direct correlation of MetS with male infertility still remains unclear. In this work, we evaluated whether MetS has a negative impact on fertility of hybrid male mice with high reproductive performance. To induce a MetS-like condition, (C57BL/6xBALB/c) F1 male mice were fed a high-fat diet (HFD, 30% fat) for 19 weeks, while controls received a normal-fat diet (NFD, 6% fat). HFD-fed animals exhibited increased body weight, hypercholesterolemia, hyperglycemia and glucose intolerance. In vivo fertilisation assays performed along the treatment period showed no differences in fertilisation nor in vitro embryo development rates between groups. While testicular weight and morphology were similar in both groups, HFD-fed mice presented lighter epididymides and higher amounts of gonadal fat. Moreover, sperm count was lower in HFD-fed mice, despite normal sperm viability, morphology, motility or acrosome reaction. Finally, no differences were observed in in vitro fertilisation rates between groups. In summary, although HFD feeding altered some reproductive parameters, it did not impair male fertility in high performance breeders suggesting the possibility that a fertility impairment could be the result of the cumulative combination of environmental and/or genetic factors.
Radiobiological effects of the alpha emitter Ra-223 on tumor cells
Authors: Bannik Kristina , Madas Balázs , Jarzombek Marco , Sutter Andreas , Siemeister Gerhard , Mumberg Dominik , Zitzmann-Kolbe Sabine 
Published: December 2019

AbstractTargeted alpha therapy is an emerging innovative approach for the treatment of advanced cancers, in which targeting agents deliver radionuclides directly to tumors and metastases. The biological effects of α-radiation are still not fully understood - partly due to the lack of sufficiently accurate research methods. The range of α-particles is <100 μm, and therefore, standard in vitro assays may underestimate α-radiation-specific radiation effects. In this report we focus on α-radiation-induced DNA lesions, DNA repair as well as cellular responses to DNA damage. Herein, we used Ra-223 to deliver α-particles to various tumor cells in a Transwell system. We evaluated the time and dose-dependent biological effects of α-radiation on several tumor cell lines by biological endpoints such as clonogenic survival, cell cycle distribution, comet assay, foci analysis for DNA damage, and calculated the absorbed dose by Monte-Carlo simulations. The radiobiological effects of Ra-223 in various tumor cell lines were evaluated using a novel in vitro assay designed to assess α-radiation-mediated effects. The α-radiation induced increasing levels of DNA double-strand breaks (DSBs) as detected by the formation of 53BP1 foci in a time- and dose-dependent manner in tumor cells. Short-term exposure (1–8 h) of different tumor cells to α-radiation was sufficient to double the number of cells in G2/M phase, reduced cell survival to 11–20% and also increased DNA fragmentation measured by tail intensity (from 1.4 to 3.9) dose-dependently. The α-particle component of Ra-223 radiation caused most of the Ra-223 radiation-induced biological effects such as DNA DSBs, cell cycle arrest and micronuclei formation, leading ultimately to cell death. The variable effects of α-radiation onto the different tumor cells demonstrated that tumor cells show diverse sensitivity towards damage caused by α-radiation. If these differences are caused by genetic alterations and if the sensitivity could be modulated by the use of DNA damage repair inhibitors remains a wide field for further investigations.
Host alarm calls attract the unwanted attention of the brood parasitic common cuckoo
Authors: Marton Attila , Fülöp Attila , Ozogány Katalin , Moskát Csaba , Bán Miklós 
Published: December 2019

AbstractIt is well known that avian brood parasites lay their eggs in the nests of other bird species, called hosts. It remains less clear, however, just how parasites are able to recognize their hosts and identify the exact location of the appropriate nests to lay their eggs in. While previous studies attributed high importance to visual signals in finding the hosts’ nests (e.g. nest building activity or the distance and direct sight of the nest from vantage points used by the brood parasites), the role of host acoustic signals during the nest searching stage has been largely neglected. We present experimental evidence that both female and male common cuckoos Cuculus canorus pay attention to their host’s, the great reed warbler’s Acrocephalus arundinaceus alarm calls, relative to the calls of an unparasitized species used as controls. Parallel to this, we found no difference between the visibility of parasitized and unparasitized nests during drone flights, but great reed warblers that alarmed more frequently experienced higher rates of parasitism. We conclude that alarm calls might be advantageous for the hosts when used against enemies or for alerting conspecifics, but can act in a detrimental manner by providing important nest location cues for eavesdropping brood parasites. Our results suggest that host alarm calls may constitute a suitable trait on which cuckoo nestlings can imprint on to recognize their primary host species later in life. Our study contributes to the growing body of knowledge regarding the context-dependency of animal signals, by providing a novel example of a beneficial acoustic trait intercepted by a heterospecific and used against the emitter.
Essential role of the initial activation signal in isotype selection upon deletion of a transcriptionally committed promoter
Authors: Santos Joana M. , Oudinet Chloé , Schöne Lisa , Dauba Audrey , Khamlichi Ahmed Amine 
Published: December 2019

AbstractClass switch recombination (CSR), which targets exclusively the constant region of the immunoglobulin heavy chain (IgH) locus, plays an important role in humoral immunity by generating different antibody effector functions. The IgH constant locus contains multiple genes controlled by isotype (I) promoters induced by extracellular signals that activate specific I promoters, leading to B cell commitment. However, it is unknown whether after initial commitment to one promoter, non-responsive I promoters are irreversibly silent or if they can be activated after exposure to their specific inducers. Here, we studied the murine cell line CH12, which is committed to produce IgA in response to TGF-β. We show that, although other promoters than Iα are transcriptionally inactive, they are not irreversibly silent. Following deletion of the committed Iα promoter by CRISPR/Cas9, other I promoters display a complex transcriptional pattern largely dependent on the initial committing signal.
An abluminal biodegradable polymer sirolimus-eluting stent versus a durable polymer everolimus-eluting stent in patients undergoing coronary revascularization: 3-year clinical outcomes of a randomized non-inferiority trial
Authors: Zhang Haijun , Zhang Xiaoping , Yin Yuxia , Zhou Chao , Deng Wei , Zhang Junwei , Hou Wenbo , Lu Shoutao , Song Caixia , Cui Xiaoshan , Wang Shenguo , Yang Fei , Liu Guang , Duan Cuihai , Ge Junbo 
Published: December 2019

AbstractThe Cordimax stent has proved non-inferior to the Cypher Select durable polymer sirolimus-eluting stent for the primary endpoint of angiographic in-stent late luminal loss and in-stent mean diameter stenosis at 9 months. The trial was designed to compare the efficacy and safety of the Cordimax stent with the Xience V stent in patients undergoing coronary revascularization. This randomized, multicenter trial enrolled 3697 patients treated with Cordimax stent (2460 patients) and Xience V stent (1237 patients). The primary efficacy endpoint was a target-lesion failure (TLF) at 1 year and the primary safety endpoint was a composite of death or myocardial infarction (MI) at 3 years. 3399 patients (91.9%) completed 3-year follow-up. At 1 year, the primary efficacy endpoint occurred in 86 (3.5%) patients in the Cordimax group versus 40 (3.2%) patients in the Xience V group (0.3% absolute risk difference, 95% CI −1.0–1.5%, Pnon-inferiority < 0.0001). At 3 years, the primary safety endpoint occurred in 39 (1.6%) patients in the Cordimax group versus 19 (1.5%) patients in the Xience V group (0.05% absolute risk difference, 95% CI −0.8–0.9%, Pnon-inferiority < 0.0001). The incidence of target lesion revascularization was low in Cordimax group compared with Xience V group (3.6% versus 5.1%, P = 0.03). There were no differences between Cordimax and Xience V in terms of Cardiac death (0.3% versus 0.4%, P = 0.70), myocardial infarction (1.2% versus 0.9%, P = 0.37), and the stent thrombosis (0.4% versus 0.6%, P = 0.61). In conclusion, safety and efficacy outcomes of Cordimax stent were non-inferior to the Xience V stent 3 years after stent implantation.
The Strength of the Movement-related Somatosensory Cortical Oscillations Differ between Adolescents and Adults
Authors: Gehringer James E. , Arpin David J. , VerMaas Jacy R. , Trevarrow Michael P. , Wilson Tony W. , Kurz Max J. 
Published: December 2019

AbstractAdolescents demonstrate increasing mastery of motor actions with age. One prevailing hypothesis is that maturation of the somatosensory system during adolescence contributes to the improved motor control. However, limited efforts have been made to determine if somatosensory cortical processing is different in adolescents during movement. In this study, we used magnetoencephalographic brain imaging to begin addressing this knowledge gap by applying an electrical stimulation to the tibial nerve as adolescents (Age = 14.8 ± 2.5 yrs.) and adults (Age = 36.8 ± 5.0 yrs.) produced an isometric ankle plantarflexion force, or sat with no motor activity. Our results showed strong somatosensory cortical oscillations for both conditions in the alpha-beta (8–30 Hz) and gamma (38–80 Hz) ranges that occurred immediately after the stimulation (0–125 ms), and a beta (18–26 Hz) oscillatory response shortly thereafter (300–400 ms). Compared with the passive condition, all of these frequency specific cortical oscillations were attenuated while producing the ankle force. The attenuation of the alpha-beta response was greater in adolescents, while the adults had a greater attenuation of the beta response. These results imply that altered attenuation of the somatosensory cortical oscillations might be central to the under-developed somatosensory processing and motor performance characteristics in adolescents.
Strain-induced control of a pillar cavity-GaAs single quantum dot photon source
Authors: Yeo Inah , Kim Doukyun , Han Il Ki , Song Jin Dong 
Published: December 2019

AbstractHerein, we present the calculated strain-induced control of single GaAs/AlGaAs quantum dots (QDs) integrated into semiconductor micropillar cavities. We show precise energy control of individual single GaAs QD excitons under multi-modal stress fields of tailored micropillar optomechanical resonators. Further, using a three-dimensional envelope-function model, we evaluated the quantum mechanical correction in the QD band structures depending on their geometrical shape asymmetries and, more interestingly, on the practical degree of Al interdiffusion. Our theoretical calculations provide the practical quantum error margins, obtained by evaluating Al-interdiffused QDs that were engineered through a front-edge droplet epitaxy technique, for tuning engineered QD single-photon sources, facilitating a scalable on-chip integration of QD entangled photons.
Production of ethanol from Jerusalem artichoke by mycelial pellets
Authors: Zhang Chao , Wu Daoji , Yang Hongqi , Ren Huixue 
Published: December 2019

AbstractMycelial pellets formed by Aspergillus niger A-15 were used to immobilize the ethanol producing yeast Saccharomyces cerevisiae C-15. The operation parameters, such as agitation speed, temperature and mixed proportion of strains were studied. The optimal adsorption 66.9% was obtained when speed was 80r/min, temperature was 40 °C and mixed proportion(mycelial pellets: yeasts) was 1:10. With Jerusalem artichoke flour as substrate, 12.8% (V/V) of ethanol was obtained after 48 h by simultaneous saccharification and fermentation using mycelial pellets. And mycelial pellets could tolerate 19% (volume fraction) ethanol. The above results proved that this new technology was feasible, and it had the advantages of higher ethanol yield, long service life, repeated use, easy operation and lower cost in producing ethanol.
Trends in United States large hail environments and observations
Authors: Tang Brian H. , Gensini Vittorio A. , Homeyer Cameron R. 
Published: December 2019

AbstractUnderstanding trends in large hail-producing environments is an important component of estimating hail risk. Here, we use two environmental parameters, the Large Hail Parameter and the Significant Hail Parameter, to assess trends in days with environments conducive for hail ≥5 cm. From 1979 to 2017, there has been an increase in days with favorable large hail environments in central and eastern portions of the U.S. This increase has been driven primarily by an increasing frequency of days with steep mid-tropospheric lapse rates and necessary combinations of instability and vertical wind shear for severe thunderstorms. Annual large hail environment area is significantly, positively correlated with (1) large hail report area east of the Rocky Mountains, and (2) large hail radar-derived area in the Midwest and Northeast. This evidence suggests that there may be an environmental fingerprint on increasing large hail risk and expanding this risk eastward.