bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunological Analysis and Differential Genes Screening of Venous Thromboembolism
Authors: Gao Li-Na , Li Qiang , Xie Jian-Qin , Yang Wan-Xia , You Chong-Ge 
Published: June 2020

Abstract
Purpose: To explore the pathogenesis of venous thromboembolism (VTE) and provide bioinformatics basis for the prevention and treatment of VTE. Methods: The R software was used to obtain the gene expression profile data of GSE19151, combining with the CIBERSORT database, obtain immune cells and differentially expressed genes (DEGs) of blood samples of VTE patients and normal control, and analyze DEGs for GO analysis and KEGG pathway enrichment analysis. Then, the protein-protein interaction (PPI) network was constructed by using the STRING database, the key genes (hub genes) and immune differential genes were screened by Cytoscape software, and the transcription factors (TFs) regulating hub genes and immune differential genes were analyzed by the NetworkAnalyst database. Results: Compared with the normal group, monocytes and resting mast cells were significantly expressed in the VTE group, while regulatory T cells were significantly lower. Ribosomes were closely related to the occurrence of VTE. 10 hub genes and immune differential genes were highly expressed in VTE. MYC, SOX2, XRN2, E2F1, SPI1, CREM and CREB1 can regulate the expressions of hub genes and immune differential genes. Conclusions: Ribosomal protein family genes are most relevant to the occurrence and development of VTE, and the immune differential genes may be the key molecules of VTE, which provides new ideas for further explore the pathogenesis of VTE.
Iron Overload Adversely Effects Bone Marrow Haematogenesis via SIRT-SOD2-MROS in a Process Ameliorated by Curcumin
Authors: zhou shujuan , Sun Lan , Qian Shanhu , Ma Yongyong , Ma Ruye , Dong Yuqing , Shi Yifen , Jiang Songfu , Ye Haige , Shen Zhijian , Zhang Shenghui , Shen Jianping , Yu Kang , wang siqian 
Published: June 2020

Abstract
Background: Iron overload is common in patients with haematological disorders, and it is known to have a suppressive effect on haematogenesis. However, the mechanism by which iron overload affects haematogenesis is still unclear. The antioxidant curcumin has been reported to protect against iron overload-induced bone marrow damage, although the mechanism underlying this protective effect remains to be elucidated.Methods: We established iron overload cell and mouse models. Mitochondrion-derived reactive oxygen species (mROS) levels, autophagy levels, and the SIRT3/SOD2 pathway were examined in these models and in the bone marrow of patients with iron overload.Results: Iron overload was shown to depress haematogenesis and induce mitochondrion-derived superoxide anion-dependent autophagic cell death. Iron loading decreased SIRT3 protein expression, promoted an increase in SOD2, and led to the elevation of mROS. These effects were reversed by the overexpression of SIRT3. Curcumin treatment ameliorated peripheral blood cells, enhanced SIRT3 activity, decreased SOD2 acetylation, inhibited mROS production, and suppressed iron loading-induced autophagy.Conclusions: These results suggest that curcumin exerts a protective effect on bone marrow by reducing mROS-stimulated autophagic cell death in a manner dependent on the SIRT3/SOD2 pathway.
Improving Mental Health Care for Unaccompanied Young Refugees through a Stepped Care Approach versus Usual Care+: Study Protocol of a Cluster Randomized Controlled Hybrid Effectiveness Implementation Trial
Authors: Rosner Rita , Sachser Cedric , Hornfeck Fabienne , Kilian Reinhold , Kindler Heinz , Muche Rainer , Müller Lauritz Rudolf Floribert , Thielemann Jonathan , Waldmann Tamara , Ziegenhain Ute , Unterhitzenberger Johanna , Pfeiffer Elisa 
Published: June 2020

Abstract
Background: More than half of the unaccompanied young refugees (UYRs) resettled in Europe report elevated levels of posttraumatic stress symptoms (PTSS) and comorbid symptoms. Earlier studies have highlighted the effectiveness of the trauma-focused preventive group intervention ‘Mein Weg’ (English ‘My Way’), and the feasibility of trauma-focused cognitive behavioral therapy (TF-CBT) for UYRs. Both interventions are deemed to be empirically supported treatments (ESTs). However, UYRs seldom receive ESTs or, in fact, any treatment at all. In view of the high need and the limited treatment resources available, a stepped-care approach is indicated but has not been evaluated so far. The purpose of this trial is to compare the stepped-care approach BETTER CARE with usual care enhanced with screening and indication (usual care+).Methods: In a cluster randomized controlled trial involving N = 540 UYRs living in up to N=54 child and youth welfare service (CYWS) facilities, BETTER CARE will be compared with usual care+. We will randomize clusters comprising a CYWS facility with at least one eligible psychotherapist. BETTER CARE consists of step 1) screening and indication and either step 2) preventive trauma-focused group intervention ‘Mein Weg’ delivered by trained CYWS staff or step 3) TF-CBT delivered by trained community therapists and supported by trained translators if necessary. Participants will be assessed 6 and 12 months after randomization. The primary outcome is the severity of PTSS after 12 months. Secondary outcomes are depressive and anxiety symptoms, quality of life, and proxy reported PTSS. Furthermore, drug use, health costs, benefits and long-term effects on integration/acculturation will be assessed. Discussion: The trial will directly integrate a stepped-care approach into existing structures of the German child welfare and (mental) health system. It could, therefore, serve as a blueprint for how to implement ESTs for UYRs. If successful, screening, prevention and intervention will be sustainably implemented in CYWS in southern Germany.Trial registration: German Clinical Trials Register, ID: DRKS00017453. Registered December 11th, 2019. URL: https://www.drks.de/drks_web/setLocale_EN.do
LBX2-AS1 Promotes Ovarian Cancer Progression by Facilitating E2F2 Gene Expression via MIR-455-5P and MIR-491-5P Sponging
Authors: Cao Jian , Wang Huan , Tang Ranran , Liu Guangquan , Xu Pengfei , Wang Huayu , Miao Juan , Gu Xiaoyan , Han Suping 
Published: June 2020

Abstract
Background:LBX2-AS1 is a long noncoding RNA that facilitates the development of gastrointestinal cancers and lung cancer, but its participation in ovarian cancer development remained uninvestigated.Methods: Clinical data retrieved from TCGA ovarian cancer database and the clinography of 60 ovarian cancer patients who received anti-cancer treatment in our facility were analyzed. The overall cell growth, colony formation, migration, invasion, apoptosis and tumor formation on nude mice of ovarian cancer cells were evaluated before and after lentiviral-based LBX2-AS1 knockdown. ENCORI platform was used to explore LBX2-AS1-interacting microRNAs and target genes of the candidate microRNAs. Luciferase reporter gene assay and RNA-pulldown assay were used to verify the putative miRNA-RNA interactions.Results: Ovarian cancer tissue specimens showed significant higher LBX2-AS1 expression levels that non-cancerous counterparts. High expression level of LBX2-AS1 significantly associated with patients reduced overall survival. LBX2-AS1 knockdown significantly downregulated the cell growth, colony formation, migration, invasion and tumor formation capacity of ovarian cancer cells and increased their apoptosis in vitro. LBX2-AS1 interacts with and thus inhibits the function of miR-455-5p and miR-491-5p, both of which restrained the expression of E2F2 gene in ovarian cancer cells via mRNA targeting. Transfection of miRNA inhibitors of these two miRNAs or forced expression of E2F2 counteracted the effect of LBX2-AS1 knockdown on ovarian cancer cells.ConclusionsLBX2-AS1 was a novel cancer-promoting lncRNA in ovarian cancer. This lncRNA increased the cell growth, survival, migration, invasion and tumor formation of ovarian cancer cells by inhibiting miR-455-5p and miR-491-5p, thus liberating the expression of E2F2 cancer-promoting gene.
Clinical characterisation and the improved molecular diagnosis of autosomal dominant cone-rod dystrophy in patients with SCA7
Authors: Zou Xuan , Yao Fengxia , Li Fengrong , Wu Shijing , Li Hui , Sun Zixi , Zhu Tian , Wei Xing , Li Donghui , Sui Ruifang 
Published: June 2020

Abstract
Purpose

To evaluate the retinal phenotype and genetic features of Chinese patients with spinocerebellar ataxia type 7 (SCA7).
Methods

Detailed ophthalmic examinations, including electroretinograms, fundus photography, fundus autofluorescence and optical coherence tomography, were performed to analyse the retinal lesions of patients with SCA7. A molecular genetic analysis was completed to confirm the number of CAG repeats in ATXN7 gene on the patients and their family members.
Results

Eight patients from three families with SCA7 were included in this study. Trinucleotide repeat was expanded from 43 to 113 in the affected patients. The affected patients were characterised by different degrees of cone-rod dystrophy, which is positively related to the number of CAG repeats and age. All patients complained of progressive bilateral visual loss, and most cases reported visual disturbance earlier than gait movement or dysarthria. A coarse granular appearance of the macular region on scanning laser ophthalmoscopy, hypofluorescence in the macula on autofluorescence, retinal atrophy on optic coherence tomography, depression of multifocal electroretinograms and prominent abnormalities in cone-mediated responses on electrograms are the general features of SCA7-related retinopathy. Hyperreflective dots in the outer retinal layers and choroidal vessel layers are a common sign in optic coherence tomography in the advanced stage.
Conclusions

SCA shows a cone-rod dystrophy phenotype. The multimodal imaging of the retina is beneficial to detect the early lesions of cone-rod dystrophy related to SCA7.
Regeneration of caudate lobe after living donor liver transplantation: Comparison with a surrogate model of left lobe graft
Authors: Saito Yu , Imura Satoru , Morine Yuji , Ikemoto Tetsuya , Yamada Shinichiro , Shimada Mitsuo 
Published: June 2020

Abstract
Background:
The aim of this study is to clarify the regeneration of the CL (caudate lobe) without any reconstructions of short hepatic veins (SHVr) after LDLT (living donor liver transplantation) and compare the regeneration of the CL after right hepatectomy (Rt. Hx), as the surrogate model of extended left lobe graft (Ex LLG) with complete SHVr.
Methods:
Eleven Ex LLGs with CL were included in this study. SHVr was not performed in all cases. The volumetry was performed before, one month and six months after LDLT. Seven patients who underwent Rt. Hx were also included in this study as the surrogate model.
Results:
In Ex LLGs with CL, the regeneration rate of the large CL (> 30 ml) was worse than that of small CL (< 30 ml). In the surrogate model, the regeneration rate of the CL was not worse than other segments. However, the regeneration rate of the large CL was also worse than that of small CL even in the presence of complete SHVr.
Conclusions:
The regeneration of the large CL was worse than that of the small CL regardless of the presence or absence of SHVr, indicating that SHVr in Ex LLG with CL might not be necessary.
Individual and Poly-Substance use and Condomless Sex Among HIV-Uninfected Adults Reporting Heterosexual Sex in a Multi-Site Cohort
Authors: Fredericksen Rob , Whitney Bridget M , Trejo E , Nance Robin , Fitzsimmons Emma , Altice Frederick , Carrico Adam , Cleland Charles , Rio Carlos Del , Duerr Ann , El-Sadr Wafaa , Kahana Shoshana , Kuo Irene , Mayer Kenneth , Mehta Shruti , Ouellet Lawrence , Quan Vu , Rich Josiah , Seal David , Springer Sandra , Taxman Faye , Wechsberg Wendee , Crane Heidi , Delaney JAC 
Published: June 2020

Abstract
BACKGROUND: We analyzed the association between substance use (SU) and condomless sex (CS) among HIV-negative adults reporting heterosexual sex in the Seek, Test, Treat, and Retain (STTR) consortium. We describe the impact of SU as well as person/partner and context-related factors on CS, identifying combinations of factors that indicate the highest likelihood of CS.METHODS: We analyzed data from four US-based STTR studies to examine the effect of SU on CS using two SU exposures: 1) current SU (within 3 months) and 2) SU before/during sex. Adjusted individual-study, multivariable relative risk regression was used to examine the relationship between CS and SU. We also examined interactions with type of sex and partner HIV status. Pooled effect estimates were calculated using traditional fixed-effects meta-analysis.RESULTS: We analyzed data for current SU (n=6781; 82% men, median age=33 years) and SU before/during sex (n=2915; 69% men, median age=40 years). For both exposure classifications, any SU other than cannabis increased the likelihood of CS relative to non-SU (8-16%, p-values<0.001). In the current SU group, however, polysubstance use did not increase the likelihood of CS compared to single-substance use. Cannabis use did not increase the likelihood of CS, regardless of frequency of use. Type of sex was associated with CS; those reporting vaginal and anal sex had a higher likelihood of CS compared to vaginal sex only for both exposure classifications (18-21%, p<0.001). Current SU increased likelihood of CS among those reporting vaginal sex only (9-10%, p<0.001); results were similar for those reporting vaginal and anal sex (5-8%, p <0.01). SU before/during sex increased the likelihood of CS among those reporting vaginal sex only (20%; p<0.001) and among those reporting vaginal and anal sex (7%; p=0.002). Single- and poly-SU before/during sex increased the likelihood of CS for those with exclusively HIV-negative partners (7-8%, p£0.02), and for those reporting HIV-negative and HIV-status unknown partners (9-13%, p£0.03).CONCLUSION: Except for cannabis, any SU increased the likelihood of CS. CS was associated with having perceived HIV-negative partners and with having had both anal/vaginal sex.
Design and Analysis of A Complete Full Adder Based On Metal-Insulator-Metal (MIM) Waveguide-Based Plasmonic Waves
Authors: yoosefi oraman , Rodríguez Angel 
Published: June 2020

Abstract
In this paper, metal-insulator-metal (MIM) plasmonic waveguide structures and a rectangular cavity resonator at a central frequency of 1550 nm were used to propose a complete full adder. Under this circumstances, the system has a fast function with slight variations in real- time or near real-time manner, and this led to its minimum power consumption, while serving in various situations. In this full adder, we benefited from the property of combining resonant waves in the first and second modes, and we managed to obtain a high transmission coefficient in states where the output must be active. This complete full adder operates through designing 4-input AND, XOR, OR, and NOT logic gates, resulting in the design of a complete full adder with low manufacturing complexity and cost relative to ones designed through combining the conventional 2-input AND and OR gates. In comparison of three computational methods, finite‐difference time‐domain (FDTD) is a simple and versatile method. This method directly discretizes the time‐domain partial differential form of Maxwell's equations in various dimensions while using analytical solution in the remaining direction and solving the 3D scattering problem. Therefore, necessary simulations were conducted using FDTD software, and showed a good fit to the results predicted through approximations intended for theoretical relations.
Correlation Of Bone Mineral Density Measured In Quantitative Computed Tomography With Hounsfield Unit
Authors: Lalruatfela R , Kotian Rahul P , Panakkal Nitika C 
Published: June 2020

Abstract
Background: Bone mineral density scan (BMD) is a simple, non-invasive procedure used to assess the strength of the bones by measuring the composition of minerals mainly calcium in the bones. In this study, BMD was measured using Quantitative Computed Tomography (QCT) and Hounsfield unit (HU) in the lumbar spine and the values were correlated.Methods: 240 participants referred for CT Abdomen and CT Lumbar spine were scanned using 64 slice Brilliance CT. Using BMD software, three different vertebral bodies from L1-L3 were taken and ROI was placed at the central portion of the trabecular bone. Two references ROI one in retro spinal muscle and one in fat tissue was also placed. To measure CT attenuation value an ROI graphic tool was drawn at the trabecular bone. The average of BMD in QCT and HU value was taken from L1-L3. Pearson Correlation Coefficient was used to correlate QCT and HU values.Results: The mean BMD for the 21-40 age group was found to be 156.3 and 228.0 for QCT and HU respectively. Similarly, the mean BMD for 41-60 and 61-80 age groups was found to be 125.5, 173.6 and 109.1, 140.4 for QCT and HU respectively. The results showed a strong positive correlation between QCT and HU BMD (r = 0.94) with a p-value less than 0.001.Discussion: In our present study, 64.53% (n=155) were found to have normal BMD based on the WHO diagnostic category for spine BMD in QCT. Whereas 24.58% were found to have a low bone mass (osteopenia) and 10.83% were found to have osteoporosis. The equivalent mean HU was found to be 211.98 ±31.06, 139.64 ±18.58, 87.22 ±15.92 for normal, osteopenia and osteoporosis respectively.Conclusion: The study shows a strong correlation between QCT BMD with HU. Therefore, the CT attenuation technique can also be used to derive bone mineral density values from routine abdomen and lumbar spine MDCT for osteoporosis screening with no additional cost to the patient
Relationships between secure attachment, experiential co-creation and future experiential intentions in the tourism industry: The case of COVID-19
Authors: Wu Hung-Che , Chang Ya-Yuan 
Published: June 2020

Abstract
This paper aims to explore the relationships between the dimensions of secure attachment, the dimensions of experiential co-creation and future experiential intentions. A convenience sample of 505 tourists who had traveled by domestic or international airlines during the COVID-19 outbreak in Hartsfield–Jackson Atlanta International Airport was surveyed. The results will assist tourism operators in developing and implementing market-orientated service strategies to increase secure identity, secure dependence, secure affect, secure social bonding, experiential trust, experiential commitment and experiential connection in order to enable tourists to have future intentions to experience the destination during the COVID-19 outbreak.
Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Infections in Southwest China
Authors: Hu Xiaopin , Yuan Guohang , Wu Yaoyao , Liu Weijia , Zhang Xiangyan , Liu Lin 
Published: June 2020

Abstract
Background: We determined epidemiological characteristics and resistance mechanisms of carbapenem-resistant Klebsiella pneumoniae (CRKP) strains found in Southwest China and assessed disease burden to provide evidence-based strategies for control and treatment of CRKP infection. Methods: A total of 159 strains of CRKP were isolated from sputa, blood, urine, ascites and wound secretions from three tertiary hospitals in Southwest China between August 1st, 2018 and December 31st, 2019. The sensitivity of each strain to 12 antibiotic agents was determined by micro-broth dilution. Identification of carbapenemase genes and multi-locus sequence typing (MLST) were performed using polymerase chain reaction (PCR). The disease burdens of patients with CRKP were assessed based on invasive procedures, antibiotic use, laboratory tests and clinical outcomes. Results: Of 159 CRKP strains analyzed, 50.9% were isolated from sputum samples. The percentage of patients who underwent invasive procedures before positive cultures for CRKP were detected was 96.3%. The mortality of blood infection was highest (66.6%) among patients with CRKP infection. All strains were insensitive to carbapenems. The resistance rates to levofloxacin and amikacin were 85.5% and 81.8%, respectively. All CRKP strains produced carbapenemases, with a majority of isolates (81.1%) producing KPC-2. The MICs of strains harbouring both KPC-2 and NDM-1 were higher than those of strains with only KPC-2 or NDM-1. ST11 is the most popular clonotype found in Southwest China. Conclusions: CRKP strains in Southwest China are characterized by strong drug resistance and associated with poor clinical prognoses. It is therefore urgent to both strengthen control measures and improve prevention awareness.
Cost of Oral Cancer: Protocol for a Systematic Review
Authors: RIBEIRO-ROTTA REJANE FARIA , Rosa Eduardo Antônio , Milani Vanessa , Dias Nadielle Rodrigues , Ferreira Daniele Masterson Tavares Pereira , Silva Everton Nunes , Zara Ana Laura de Sene Amâncio 
Published: June 2020

Abstract
Background: Cancer care is among the costliest in the social sphere. Oral cancer (OC) is a chronic disease whose incidence has been growing worldwide. The lack of economic evaluation studies assessing the economic impact of OC, as well as the need to search for evidence of the economic burden of OC motivated this investigation. This study aims to collect evidence on the cost of OC (lip cancer - LC, oral cavity cancer - OCC and oropharyngeal cancer - OPC) worldwide. Methods: A systematic review will be conducted take into consideration any perspective. The primary outcome will be the costs related to oral cancer in patients, reported in monetary units. Specific breakdown of costs will be reported (direct hospital costs, rehabilitation care costs, direct non-healthcare costs, indirect costs and productivity loss, and intangible costs). SCOPUS, WEB OF SCIENCE, COCHRANE and BVS databases will be searched. Original studies on cost of OC that assess its cost based on components as: hospital and/or outpatient, promotion and prevention will be included. It will be excluded types of study such as: editorial, letters, reviews, case reports, case series, clinical trials, studies that address specific analysis, such as cost-effectiveness, cost-utility, cost-benefit, cost-minimization. A systematic meta-narrative synthesis will be presented. Data on quantities of health and social resource consumption, currencies used and whether studies show total or incremental costs will be collected.Discussion: The identification of evidence on the economic burden of LC, OCC and OPC will allow the determination of the contribution share of each sector of society in the total economic burden of oral cancer. Also, this evidence will favour the estimate of the impact that the reduction of the OC burden would have on its associated costs and better guide the choice decision, to optimize the benefits. Systematic review registration: PROSPERO ID number 172471.