bluuebunny/crossref_metadata_2025_split huggingface dataset:
Direct-Acting Oral Anticoagulant Removal by Intraoperative Hemoadsorption in CABG and/or Single Valve Surgery: Interim Analysis of the International Safe and Timely Antithrombotic Removal (STAR) Registry
Authors: Schmoeckel Michael , Thielmann Matthias , Vitanova Keti , Eberle Thomas , Marczin Nandor , Hassan Kambiz , Liebold Andreas , Lindstedt Sandra , Mächler Georg , Matejic-Spasic Marijana , Wendt Daniel , Deliargyris Efythymios , Storey Robert 
Published: December 2024

Abstract

Objective
Patients on direct-acting oral anticoagulants (DOACs) are at high risk of perioperative bleeding complications. Intraoperative hemoadsorption is a novel strategy to reduce perioperative bleeding in patients on DOACs undergoing non-deferable cardiac surgery. The international STAR-registry reports real-world clinical outcomes associated with this application.
Methods
The hemoadsorption device was incorporated into the cardiopulmonary bypass (CPB) circuit and active for the duration of the pump run. Patients on DOACs undergoing CABG and/or single valve surgery before completing the recommended washout were included. Outcome measurements included bleeding events according to standardized definitions and 24-hour chest-tube-drainage (CTD).
Results
A total of 62 patients were included from 7 institutions in Austria, Germany, Sweden, and the UK (mean age 69.9 ± 7.5years, 71% male). Approximately half were on apixaban and the other half was split between rivaroxaban and edoxaban with 21% of patients also on aspirin. Surgery occurred at a median time of 28.9h since the last DOAC dose with single valve surgery accounting for 2/3 of cases. Mean CPB duration was 118.6 ± 46.4min. Severe bleeding (UDPB ≥ 3) occurred in 4.8%, and BARC-4 bleeding occurred in 3.2% of the patients. Only one patient (1.6%) required reoperation for bleeding control. The mean 24-hour CTD was 771.3 ± 482.79mL. No device-related adverse events were reported.
Conclusions
This interim report of the ongoing STAR-registry shows that, in patients on DOAC undergoing non-deferable CABG and/or single valve surgery, the use of intraoperative hemoadsorption is associated with low rates of severe perioperative bleeding complications.
Clinical registration number:ClinicalTrials.gov identifier: NCT05077124
Association between the triglyceride-glucose obesity-related index and risk of gallstone disease and first gallstone surgery: the NHANES 2017-2020
Authors: Zhang Yue , Duan Ruifeng , Chen Xin , Wei lijuan 
Published: December 2024

Abstract

Background
The triglyceride-glucose index (TyG) and its associated obesity indicators [TyG-body mass index(TyG-BMI), TyG-waist-height ratio(TyG-WHtR), and TyG-waist circumference(TyG-WC)] are emerging tools for assessing insulin resistance. This study aimed to examine the association between TyG obesity-related indicators and the risk of gallstone disease or first gallbladder surgery.
Methods
Individuals were selected from the 2017–2020 National Health and Nutrition Examination Survey (NHANES) database. The associations between TyG, TyG-WHtR, TyG-WC, TyG-BMI, and the risk of gallstone disease and first gallbladder surgery were initially analyzed using weighted logistic regression models. Restricted cubic spline (RCS) plots and receiver operating characteristic (ROC) curves were used to analyze the relationship between TyG obesity-related indicators and gallstone disease and first gallbladder surgery. Generalized additive models, smoothed curve fitting, and linear regression were applied to assess the relationship between TyG-WC and age at first gallbladder surgery. Sensitivity analyses, including stratified and interaction analyses, were conducted to identify specific populations and assess potential interactions between covariates and TyG obesity-related indicators. Mediation analyses explored the role of ethnicity as a mediator between TyG-WHtR and first gallbladder surgery.
Results
In this study, TyG obesity-related indicators were significantly correlated with the risk of gallstone disease and first gallbladder surgery, except for TyG, which showed no significant correlation. A linear relationship was observed between TyG obesity-related indicators and the risk of gallstone disease (P-overall < 0.0001, P-nonlinear > 0.05). In addition, TyG-WC demonstrated the strongest performance in predicting the risk of first gallbladder surgery and was negatively associated with age at first gallbladder surgery (β = -0.03, 95% CI: -0.04, -0.01). Sensitivity analysis indicated that TyG-WHtR was more strongly correlated with first gallbladder surgery in other races (OR = 3.13, 95% CI: 1.67, 5.86). Mediation analyses showed that race mediated the association between TyG-WHtR and first gallbladder surgery.
CONCLUSION
TyG-WHtR, TyG- WC, and TyG-BMI were correlated and linearly associated with gallstones and first gallbladder surgery. In addition TyG- WC showed the most favourable performance in predicting the risk of first gallbladder surgery and may lead to an earlier age of first gallbladder surgery. In addition attention needs to be paid to the effect of other races between TyG-WHtR and first gallstone surgery.
CAMK2B affects the proliferation, invasion and migration of glioma cells via Ras/Raf/MEK/ERK signal pathway
Authors: Zhang Shiyang , Li Jingchen , Jin Qianxu , Zhu Siyu , Yan Hongshan , Wang Yizheng , Song Zihan , Liu Liqiang 
Published: December 2024

Abstract

Background
Glioma exhibits a high recurrence rate and unfavorable prognosis, with the mechanisms underlying the regulation of glioma cell proliferation, invasion, and migration remaining elusive. CAMK2B, a crucial kinase involved in regulating cell growth and synaptic plasticity, remains enigmatic in terms of its specific role in glioma.
Methods
This study comprehensively analyzed the correlation between the expression level of CAMK2B in gliomas and patient prognosis using immunohistochemistry, qRT-PCR, and Western Blot techniques. Furthermore, the study determined the role of CAMK2B in glioma cell proliferation, invasion, and migration through CCK8, EdU, wound healing, Transwell, and in vivo tumor xenograft assays.
Result
We observed that patients exhibiting high levels of CAMK2B exhibited superior prognostic outcomes compared to those with low levels. Furthermore, CAMK2B expression was notably lower in glioma tissues and cells compared to both normal brain tissue and human astrocyte cell lines. Notably, overexpression of CAMK2B in glioma cells suppressed their proliferative capacity by approximately 40% and attenuated their invasive and migratory abilities by 60–70%, relative to normal glioma cell lines. Conversely, knockdown of CAMK2B using siRNA-CAMK2B significantly enhanced the proliferative, invasive, and migratory capabilities of glioma cells in both in vitro and in vivo settings, increasing these abilities by 1.5 to 3 times. Remarkably, these effects could be reversed through the application of the Ras pathway inhibitor Salirasib. Western blot analysis revealed that knockdown of CAMK2B activated the Ras/Raf/MEK/ERK signaling pathway in glioma cell lines, whereas overexpression of CAMK2B exerted inhibitory effects on this pathway.
Conclusion
CAMK2B inhibits glioma proliferation, invasion, and migration through Ras/Raf/MEK/ERK signal pathway.
Reactive Power Dispatch Method for Wind Farms Based on Dynamic Weight Adjustment of Electrical Distance and Reactive Power Capacity
Authors: Wu Guodong , Li Xiaohu , Zhang Ruixiao , Bao Guangqing , tan hongtao 
Published: December 2024

Abstract

To solve the problem of terminal voltage of wind turbines (WT) exceeding the limit caused by reactive power allocation in wind farms, this paper proposes a reactive power allocation method based on dynamic weight adjustment of inverter capacity and electrical distance. Firstly, the impact of WT power output on the voltage of each node in the system at different electrical distances was analyzed. Then, establish a reactive power dispatch model that takes the remaining capacity of the converter and electrical distance factors into account, and the weight coefficient between the two is dynamically adjusted based on voltage deviation by combining the idea of disturbance observation, achieve rapid distribution of reactive power commands without relying on detailed power flow modeling of the system. Finally, a 5MW*25 wind farm case is used to verify the proposed method. The results show that the proposed method can effectively reduce the voltage deviation by more than 30% under the same reactive power scheduling instruction, and can improve the reactive power dispatch range of the wind farm.
Macrophage M2 polarization with down-regulated NOX2 promotes placental angiogenesis
Authors: Zhang Ruonan , Zhang Lu , Wang Ruixue , Wubulikasimu Ayinisa , Wei Mengtian , Tian Haojun , Yang Jialu , Wei Xuanchen , Deng Xinyu , Bi Ruomeng , Duan Tao , Wang Kai 
Published: December 2024

Abstract

The polarised state of placental Hofbauer cells (HBCs) is frequently accompanied by alterations in inflammatory factors and marker proteins, which are crucial in normal pregnancy. NOX2, as one of NADPH oxidase, is commonly expressed in phagocytes and mediates oxidative stress. A recent paper reported that the upregulation of NOX2 in porcine placenta led to reduced angiogenesis through increased oxidative stress, which attracted our interesting. To investigate whether NOX2 expression in human placenta is the cause of reduced vascularity in placentas of Fetal growth restriction (FGR), we examined the expression and localisation of NOX2 in placental villous and analysed the expression of NOX2 in different macrophages subtypes as well as its effect on vascular endothelial function. Our results showed that NOX2 was predominantly expressed in HBCs of placental villi. Moreover M2 polarisation of macrophages were inversely associated with NOX2 expression and promoted angiogenesis. The expression of NOX2 was significantly elevated in the placenta of FGR compared with normal placentas, which might be related to the reduction of placental vasculature in FGR. These results suggested that NOX2 can be used as a marker of HBCs and as a result of M2 polarization, and is also deeply involved in the functional regulation of placental vascular endothelial cells. These evidences providing a potential target for the diagnosis and treatment of FGR.
Cytoskeleton disruption and plasma membrane damage determine methuosis of normal and malignant cells
Authors: Gao Xiuge , Dong Bin , Xiao Jing , Wang Junqi , Song Xinhao , Ji Hui , Peng Jiurong , Weng Xinru , Guo Dawei , Jiang Shanxiang 
Published: December 2024

Abstract

Methuosis represents a novel cell death modality characterized by catastrophic cytoplasmic vacuolization in normal and malignant cells. However, the critical role and the underlying mechanism of cytoskeleton and plasma membrane damage in methuotic cells are largely unknown. Herein, maduramicin-treated myocardial cells (H9c2) and indole chalcone-exposed glioma cells (U251) were used as methuosis model to uncover this secret. We found that cytoskeleton protein F-actin, α-tubulin, β-tubulin and filamin A/B were disrupted in a reversible-dependent manner. In addition, RhoA-ROCK1 signaling pathway mediated cytoskeleton disruption in methuotic cells. Excessive cytoplasmic vacuolization triggered cellular plasma membrane damage and the release of DAMPs, including LDH, ATP and CRT. Furthermore, at the end phase of methuotic cells, plasma membrane was damaged independent of pore-forming protein p-MLKL and GSDMD. Endosomal sorting complex required for transport (ESCRT)-Ⅲ especially its subunit CHMP3 and CHMP5 negatively regulated excessive vacuolization-induced plasma membrane damage in cells undergoing methuosis. In conclusion, for the first time, the critical role and potential mechanism of cytoskeleton and plasma membrane damage in methuotic cells are known, which would facilitate the employment of methuosis in life science and pharmacology.
Descending Inhibitory Neurons of the RVM Cause Widespread Bilateral Antinociception and Contribute to the Pain-Inhibits-Pain Phenomenon
Authors: Zeilhofer Hanns Ulrich , Ganley Robert , Sousa Marilia , Ranucci Matteo , Beccarini Camilla , Werder Kira , Pietrafesa Francesca , d'Aquin Simon , Öztürk Tugce , Hubli Michèle , Schweinhardt Petra , Hoon Mark , Wildner Hendrik 
Published: December 2024

Abstract

Acute painful stimuli applied to one body site reduce pain at other sites. The circuit basis of this “pain-inhibits-pain” phenomenon, also known as diffuse noxious inhibitory control (DNIC) in animals or conditioned pain modulation (CPM) in humans, is largely unknown. Using anatomical and optogenetic circuit tracing, we identified a population of descending inhibitory neurons of the rostral ventromedial medulla (RVM) that densely and bilaterally innervate the spinal cord along its rostrocaudal axis. Activating these neurons reduced heat and cold sensitivity widely in healthy mice and caused similarly wide-spread antihyperalgesia in chronic pain models, while their silencing evoked mechanical allodynia and spontaneous pain-like behaviors. Noxious stimuli activated subsets of these neurons in the lateral paragigantocellularis nucleus (LPGi), which inhibited nociception upon chemogenetic reactivation. Spinally projecting inhibitory RVM neurons are hence ideally positioned to function as circuit elements of DNIC and CPM, while their dysfunction may contribute to wide-spread chronic pain syndromes.
Cross-Domain Tuple and Random Collision GuidedApproach for Creative Requirement GenerationUsing LLM
Authors: Huang ZhiJian , Zhang Junyi , Jin Min , Gong HongWu , Pi Yuyan 
Published: December 2024

Abstract

In software engineering, creative requirements are unique features detailed in software specifications. Generating these requirements is both crucial and challenging within the field of software requirements engineering. Manual methods, which rely on limited human experience, are often time-consuming and unpredictable. Current machine learning solutions typically offer only combinatorial creation mechanisms and lack objective standards for evaluating creativity. This paper presents a novel approach for automatically generating creative software requirements using large language models (LLM). Our method utilizes cross-domain tuple random collision, where innovative requirements are produced by inputting randomly recombined and preprocessed subject-verb-object (SVO) tuples into a fine-tuned GPT-2 model. We provide a detailed discussion of strategies for recombining SVO tuples from various domains and introduce techniques to enhance creativity. To evaluate the creativity of the generated requirements, we establish the GRUEN Integrated Creativity (GIC) evaluation standard by incorporating the GRUEN model. Experimental studies across three software domains show that this fully automated approach consistently generates creative requirements. Additionally, comparisons with state-of-the-art methods and ChatGPT 4.0 highlight the unique advantages of our approach.
The Psychopathological Effects of Child Marriages and the Relationship with Sociodemographic-Obstetric Factors
Authors: Teze Saniye , Ayhan Fatma , Balsak Habip 
Published: December 2024

Abstract

Purpose
The aim of this study was to determine the psychopathological characteristics of child brides and to examine the relationship with sociodemographic and obstetric factors.
Method
The sample in this descriptive, cross-sectional study consisted of 92 women who had married before the age of 18 and who were contacted via snowball sampling, one of the purposive sampling methods. Data were collected using questions concerning sociodemographic and obstetric characteristics and the Brief Symptom Inventory(BSI) to determine psychopathologic characteristics.
Results
Women whose income was lower than their expenses had higher somatization scores than those whose income exceeded expenses, and those whose mothers married under the age of 18 had higher somatization scores than those whose mothers married over 18. Women who rated their marital relationship status as moderate registered higher negative self-scores than those who rated it as poor, while those who rated their marital relationship status as good exhibited lower total and somatization scores than those who rated it as poor(p < 0.05). A weak positive correlation was observed between the duration of marriage and somatization scores. A weak negative correlation was determined between the mothers' Anxiety, Depression, Hostility and total BSI scores and age at first pregnancy, and between age at birth of the first infant and BSI total and subscale scores.
Conclusion
This study emphasizes the adverse effects of factors such as low income and early marriage on mental health. The findings may contribute to a better understanding of the psychological consequences of child marriages.
Genomic Blueprint of Novel Slow-Growing Bacteria with Insights into their Potential Adaptation Mechanisms in Soil Ecosystems
Authors: Gonçalves Osiel Silva , Santana Mateus Ferreira 
Published: December 2024

Abstract

Slow-growing bacteria play a crucial role in soil ecosystems, yet our understanding of their persistence in complex environments remains limited. Do these organisms lack essential metabolic genes, or do they possess specific genes that enable survival under challenging conditions? Our study begins to fill this knowledge gap by analyzing the genomes of four slow-growing bacteria isolated from tropical savanna soil, providing insights into their metabolic capacities and adaptation mechanisms. Our findings revealed that these bacteria represent novel species, including new strains of Bosea, Nocardioides, Cupriavidus, and Enterobacter roggenkampii. Most genomes encoded key genes and pathways related to central metabolism, particularly in sugar and amino acid metabolism, suggesting that slow growth may be an adaptive strategy. The strains also exhibited potential contributions to vital biogeochemical processes, including carbon, nitrogen, and sulfur cycling, as well as plant growth promotion. We identified 164 defense genes across 32 defense families and at least one antimicrobial resistance (AMR) gene in each strain, with strain CER55 showing the highest number of predicted AMR genes. Additionally, twelve biosynthetic gene clusters were identified in three strains. The combination of defense mechanisms, antimicrobial resistance, and secondary metabolite production indicates that these bacteria may evolved multiple strategies to thrive in competitive microbial communities.
Perceiving Altered Reality (REALTER): Understanding the Impact of Maculopathy on Reading with Extended Reality
Authors: Barbieri Mattia , Albanese Giulia A. , Sabatini Silvio P. , Gori Monica , Facchini Valentina , Sandini Giulio 
Published: December 2024

Abstract

Central vision loss due to binocular maculopathy significantly impairs daily tasks like reading by disrupting oculomotor behavior, particularly saccadic movements. This study employed the REALTER system, an extended reality simulator, to replicate severe binocular maculopathy in eighteen healthy participants. Using the MNREAD Acuity Chart, we measured reading speed and acuity, and col- lected data on ocular movements to verify the system’s capability as a tool for accurate saccade detection. Importantly, the study highlights that the read- ing difficulties caused by maculopathy result in a decline in visual acuity and reading speed, similar to that observed in individuals actually affected by this condition. Our findings revealed significant changes in saccadic patterns, includ- ing increased frequency and amplitude, which closely mirrored those observed in actual patients. We believe that these results suggest REALTER as an effec- tive tool for studying low vision by using simulated impairments in healthy individuals. This approach avoids the need to stress real patients with macu- lopathy during the acquisition of oculomotor knowledge. Additionally, it provides valuable insights into compensatory mechanisms and enhances the training of ophthalmologists in understanding the effects of low vision.
Loss of Paneth cell contact starts a WNT differentiation timer in intestinal crypts
Authors: Zon Jeroen van , Kok Rutger , Zheng Xuan , Spoelstra Willem , Clement Tinka , de Grift Yorick van , Clevers Hans , Amerongen Renee van , Tans Sander 
Published: December 2024

Abstract

WNT signaling is pivotal to the renewal of many organs including the intestine. WNT gradients are thought to provide a spatial differentiation cue, and hence control the required spatial patterning of cell types. Testing this mechanism is difficult, however, as current methods are static while the underlying processes are highly dynamic. Here, we show that a ‘timer’ mechanism rather than spatial cues controls the WNT signaling decreases that drive cell differentiation. Using live-cell imaging and single-cell tracking of intestinal organoids, we find that WNT signaling in stem cells decreases immediately after loss of Paneth cell contact. This WNT signaling decrease does not depend on cell growth and division, and precedes differentiation, as shown by subsequent antibody staining and cell type propagation along lineage trees. Cells frequently regain Paneth cell contact, which in turn increases WNT signaling again, showing that differentiation is not progressive but rather repeatedly reset. Our WNT timer mechanism overturns existing spatial gradient models, explains differentiation within the intestinal crypt, and may well be more broadly relevant to WNT-mediated tissue renewal.
Genotype-integrated single-cell transcriptome analysis of a myelodysplastic neoplasm case reveals the role of DDX41 p.R525H
Authors: Matsui Hirotaka , Nagamachi Akiko , Koizumi Minori , Kudo Rei , Ajiro Masahiko , Harada Hironori , Harada Yuka , Shichino Shigeyuki , Yoshimi Akihide 
Published: December 2024

Abstract

DEAD-box helicase 41 (DDX41) is a key gene in germline-predisposed myeloid neoplasms, where pathogenic germline variants often lead to disease after acquiring a somatic mutation in trans. We conducted single-cell RNA sequencing combined with genotyping of the p.R525 site in a myelodysplastic neoplasm (MDS) case with germline p.R339C and somatic p.R525H variants, which enabled us to compare p.R525H cells with germline variant-only cells. p.R525H acquisition selectively induced G2/M arrest in colony-forming unit-erythroid cells along with R-loop elevation, thus impairing erythropoiesis via DNA damage accumulation. In hematopoietic stem cell (HSC) and myeloid progenitor populations, gene expression profiles were largely similar between p.R525H-positive and -negative cells; however, HSC frequencies were lower and granulocyte-monocyte progenitor frequencies were higher in p.R525H cells. Ligand-receptor and transcriptional regulation analyses suggested non-cell-autonomous influence from p.R525H cells on germline variant-only cells, driving convergence toward a shared expression profile and revealing an intricate interplay that shapes the MDS phenotype.