bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silver nanoparticle conductive inks: synthesis, characterization, and fabrication of inkjet-printed flexible electrodes
Authors: Fernandes Iara J. , Aroche Angélica F. , Schuck Ariadna , Lamberty Paola , Peter Celso R. , Hasenkamp Willyan , Rocha Tatiana L. A. C. 
Published: June 2020

AbstractFlexible electronics can be developed with a low-cost and simple fabrication process while being environmentally friendly. Conductive silver inks have been the most applied material in flexible substrates. This study evaluated the performance of different conductive ink formulations using silver nanoparticles by studying the material properties, the inkjet printing process, and application based on electrical impedance spectroscopy using a buffer solution. Silver nanoparticles synthesis was carried out through chemical reduction of silver nitrate; then, seven conductive ink formulations were produced. Properties such as resistivity, viscosity, surface tension, adhesion, inkjet printability of the inks, and electrical impedance of the printed electrodes were investigated. Curing temperature directly influenced the electrical properties of the inks. The resistivity obtained varied from 3.3 × 100 to 5.6 × 10−06 Ω.cm. Viscosity ranged from 3.7 to 7.4 mPa.s, which is suitable for inkjet printing fabrication. By using a buffer solution as an analyte, the printed electrode pairs presented electrical impedance lower than 200 Ω for all the proposed designs, demonstrating the potential of the formulated inks for utilization in flexible electronic devices for biological sensing applications.
Proteome and transcriptome reveal the involvement of heat shock proteins and antioxidant system in thermotolerance of Clematis florida
Authors: Jiang Changhua , Bi Yuke , Mo Jianbin , Zhang Ruyao , Qu Mingnan , Feng Shucheng , Essemine Jemaa 
Published: June 2020

AbstractClematis florida Thun (CfT) is an ornamental and medicinal plant. It is a cold resistant but heat sensitive species and deserves to be further investigated to improve its adaptability to heat stress. Exploring the molecular mechanism potential via an omic-analysis constitutes a promising approach towards improving heat tolerance of CfT. Two CfT lines, heat resistance (HR) and heat sensitive (HS), with differential thermotolerance capacities were used for the integrative analyses of proteomics and transcriptomes. Transcriptomes analysis showed that various pathways were significantly enriched including plant hormone signal transduction and carbon fixation pathways in prokaryotes. Proteomics study revealed the enrichment of some other pathways comprising antioxidant activity and carbohydrates metabolism. Based on combined transcriptomes and proteomics analyses and following heat stress treatment, a total of 1724 annotated genes were overlapped between both CfT lines. Particularly, 84 differential expressed genes (DEGs) were overlapped in both CfT lines. Fifteen out of these 84 genes were up-regulated solely for HR line (PS) but not for HS one (SG). This strongly suggests a potential prominent role for these genes in the thermotolerance process in PS line. We corroborate that two Hsps (Hsp18 and Hsp70) out of 20 detected proteins with higher expression levels in PS than in SG based on either global transcripts or proteins levels. According to the transcriptomes and proteomics analyses, 6 proteins and their corresponding genes were found to be significantly abundant in HR line (PS). Data are available via ProteomeXchange with identifier PXD018192. The expressions levels of these 6 genes were checked also for both CfT lines to evaluate their potential contributions in the heat tolerance process. Thus, their expression levels were approximately 2~4 times higher in HR than in HS line. We provided as well a representative schematic model to highlight the key genes involved in ROS scavenging and photorespiratory pathway in CfT. This model could be helpful also in understanding the mechanism of heat tolerance in CfT.
Duplication and divergence: New insights into AXR1 and AXL functions in DNA repair and meiosis
Authors: Martinez-Garcia Marina , Fernández-Jiménez Nadia , Santos Juan L. , Pradillo Mónica 
Published: June 2020

AbstractRubylation is a conserved regulatory pathway similar to ubiquitination and essential in the response to the plant hormone auxin. In Arabidopsis thaliana, AUXIN RESISTANT1 (AXR1) functions as the E1-ligase in the rubylation pathway. The gene AXR1-LIKE (AXL), generated by a relatively recent duplication event, can partially replace AXR1 in this pathway. We have analysed mutants deficient for both proteins and complementation lines (with the AXR1 promoter and either AXR1 or AXL coding sequences) to further study the extent of functional redundancy between both genes regarding two processes: meiosis and DNA repair. Here we report that whereas AXR1 is essential to ensure the obligatory chiasma, AXL seems to be dispensable during meiosis, although its absence slightly alters chiasma distribution. In addition, expression of key DNA repair and meiotic genes is altered when either AXR1 or AXL are absent. Furthermore, our results support a significant role for both genes in DNA repair that was not previously described. These findings highlight that AXR1 and AXL show a functional divergence in relation to their involvement in homologous recombination, exemplifying a duplicate retention model in which one copy tends to have more sub-functions than its paralog.
Body size trends in response to climate and urbanization in the widespread North American deer mouse, Peromyscus maniculatus
Authors: Guralnick Robert , Hantak Maggie M. , Li Daijiang , McLean Bryan S. 
Published: June 2020

AbstractBody size decline is hypothesized to be a key response to climate warming, including warming driven by urban heat islands. However, urbanization may also generate selective gradients for body size increases in smaller endotherms via habitat fragmentation. Here we utilize a densely sampled, multi-source dataset to examine how climate and urbanization affect body size of Peromyscus maniculatus (PEMA), an abundant rodent found across North America. We predicted PEMA would conform to Bergmann’s Rule, e.g. larger individuals in colder climates, spatially and temporally. Hypotheses regarding body size in relation to urbanization are less clear; however, with increased food resources due to greater anthropogenic activity, we expected an increase in PEMA size. Spatial mixed-models showed that PEMA conform to Bergmann’s Rule and that PEMA were shorter in more urbanized areas. With the inclusion of decade in mixed-models, we found PEMA mass, but not length, is decreasing over time irrespective of climate or population density. We also unexpectedly found that, over time, smaller-bodied populations of PEMA are getting larger, while larger-bodied populations are getting smaller. Our work highlights the importance of using dense spatiotemporal datasets, and modeling frameworks that account for bias, to better disentangle broad-scale climatic and urbanization effects on body size.
Ancient DNA of Guinea Pigs (Cavia spp.) Indicates a Probable New Center of Domestication and Pathways of Global Distribution
Authors: Lord E. , Collins C. , deFrance S. , LeFebvre M. J. , Pigière F. , Eeckhout P. , Erauw C. , Fitzpatrick S. M. , Healy P. F. , Martínez-Polanco M. F. , Garcia J. L. , Ramos Roca E. , Delgado M. , Sánchez Urriago A. , Peña Léon G. A. , Toyne J. M. , Dahlstedt A. , Moore K. M. , Laguer Diaz C. , Zori C. , Matisoo-Smith E. 
Published: June 2020

AbstractGuinea pigs (Caviaspp.) have a long association with humans. From as early as 10,000 years ago they were a wild food source. Later, domesticatedCavia porcelluswere dispersed well beyond their native range through pre-Columbian exchange networks and, more recently, widely across the globe. Here we present 46 complete mitogenomes of archaeological guinea pigs from sites in Peru, Bolivia, Colombia, the Caribbean, Belgium and the United States to elucidate their evolutionary history, origins and paths of dispersal. Our results indicate an independent centre of domestication ofCaviain the eastern Colombian Highlands. We identify a Peruvian origin for the initial introduction of domesticated guinea pigs (Cavia porcellus) beyond South America into the Caribbean. We also demonstrate that Peru was the probable source of the earliest known guinea pigs transported, as part of the exotic pet trade, to both Europe and the southeastern United States. Finally, we identify a modern reintroduction of guinea pigs to Puerto Rico, where local inhabitants use them for food. This research demonstrates that the natural and cultural history of guinea pigs is more complex than previously known and has implications for other studies regarding regional to global-scale studies of mammal domestication, translocation, and distribution.
Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence
Authors: Prakash John , Mishra Ashok Kumar 
Published: June 2020

AbstractQualitative and quantitative display of multiple fluorescent analytes is made simple and reliable in this micelle assisted methodology. The adopted method involves micelle assisted evincing of ppb level of PAHs in water; measurement of total fluorescence (white light excitation fluorescence, WLEF) and data deciphering using multivariate analysis. This protocol yields sensitive and accurate quantification of the cancerous pollutants (PAHs) in aqueous media with Limit of Quantification of the order 1–10 μg/L and accuracy of >98%. The use of WLEF enables the simultaneous acquisition of fluorescence signatures of all the PAHs. It has the additional advantage of being portable, layman-friendly and cost-effective. The optimized amount of surfactants for the simultaneous extraction of PAHs from real samples was estimated as 27.8 mg (19.3 mM) of SDS and 9.1 mg (5 mM) of CTAB. Also, the analytical fidelity of the quantification such as percentage recovery (98 ± 2%), linear dynamic range (2–250 μg/L), RMSEP (<0.5), etc. explains the veracity of methodology.
Ganoderma lucidum aqueous extract prevents hypobaric hypoxia induced memory deficit by modulating neurotransmission, neuroplasticity and maintaining redox homeostasis
Authors: Sharma Purva , Tulsawani Rajkumar 
Published: June 2020

AbstractOxidative stress due to hypobaric hypoxia at extreme altitudes causes severe neuronal damage and irreversible cognitive loss. Owing to contraindications of current drug therapies, the aim of the study was to investigate memory enhancing potential of aqueous extract ofGanoderma lucidum(GLAQ) and underlying neuroprotective mechanism using rat hypobaric hypoxia test model. Rats exposed to hypobaric hypoxia showed deranged spatial memory in morris water maze test with hippocampal damage and vasogenic cerebral edema. All these changes were prevented with GLAQ treatment. Blood and biochemical analysis revealed activation of hypoxic ventilatory response, red blood cells induction, reversal of electrolyte and redox imbalance, and restoration of cellular bioenergetic losses in GLAQ treated animals. Notably, GLAQ treatment ameliorated levels of neurotransmitters (catecholamines, serotonin, glutamate), prevented glucocorticoid and α-synuclein surge, improved neuroplasticity by upregulating CREB/p-CREB/BDNF expression via ERK1/ERK2 induction. Further, restoration of nuclear factor erythroid 2-related factor with stabilization of hypoxia inducible factors and inflammatory markers were evidenced in GLAQ treated rats which was additionally established in gene reporter array using an alternative HT22 cell test model. Conclusively, our studies provide novel insights into systemic to molecular level protective mechanism by GLAQ in combating hypobaric hypoxia induced oxidative stress and memory impairment.
Motor imagery training speeds up gait recovery and decreases the risk of falls in patients submitted to total knee arthroplasty
Authors: Zapparoli Laura , Sacheli Lucia Maria , Seghezzi Silvia , Preti Matteo , Stucovitz Elena , Negrini Francesco , Pelosi Catia , Ursino Nicola , Banfi Giuseppe , Paulesu Eraldo 
Published: June 2020

AbstractWith Motor imagery (MI), movements are mentally rehearsed without overt actions; this procedure has been adopted in motor rehabilitation, primarily in brain-damaged patients. Here we rather tested the clinical potentials of MI in purely orthopaedic patients who, by definition, should maximally benefit of mental exercises because of their intact brain. To this end we studied the recovery of gait after total knee arthroplasty and evaluated whether MI combined with physiotherapy could speed up the recovery of gait and even limit the occurrence of future falls. We studied 48 patients at the beginning and by the end of the post-surgery residential rehabilitation program: half of them completed a specific MI training supported by computerized visual stimulation (experimental group); the other half performed a non-motoric cognitive training (control group). All patients also had standard physiotherapy. By the end of the rehabilitation, the experimental group showed a better recovery of gait and active knee flexion-extension movements, and less pain. The number of falls or near falls after surgery was significantly lower in the experimental group. These results show that MI can improve gait abilities and limit future falls in orthopaedic patients, without collateral risks and with limited costs.
Human metaphase chromosome consists of randomly arranged chromatin fibres with up to 30-nm diameter
Authors: Wako Toshiyuki , Yoshida Akiyo , Kato Jun , Otsuka Yuji , Ogawa Shinichi , Kaneyoshi Kohei , Takata Hideaki , Fukui Kiichi 
Published: June 2020

AbstractDuring cell division, mitotic chromosomes assemble and are equally distributed into two new daughter cells. The chromosome organisation of the two chromatids is essential for even distribution of genetic materials. Although the 11-nm fibre or nucleosome structure is well-understood as a fundamental fibrous structure of chromosomes, the reports on organisation of 30-nm basic chromatin fibres have been controversial, with debates on the contribution of 30-nm or thicker fibres to the higher order inner structure of chromosomes. Here, we used focused ion beam/scanning electron microscopy (FIB/SEM) to show that both 11-nm and 30-nm fibres are present in the human metaphase chromosome, although the higher-order periodical structure could not be detected under the conditions employed. We directly dissected the chromosome every 10-nm and observed 224 cross-section SEM images. We demonstrated that the chromosome consisted of chromatin fibres of an average diameter of 16.9-nm. The majority of the chromatin fibres had diameters between 5 and 25-nm, while those with 30-nm were in the minority. The reduced packaging ratio of the chromatin fibres was detected at axial regions of each chromatid. Our results provide a strong basis for further discussions on the chromosome higher-order structure.
Internet use in old age predicts smaller cognitive decline only in men
Authors: Ihle Andreas , Bavelier Daphne , Maurer Jürgen , Oris Michel , Kliegel Matthias 
Published: June 2020

AbstractInternet use provides cognitive stimulation and thereby may contribute to the accumulation of cognitive reserve that is proposed to be instrumental for maintaining cognitive health in ageing. As the first study so far, we examined possible gender differences in the relationship between Internet use and subsequent cognitive decline over six years assessed through changes in Trail Making Test (TMT) accomplishment time in 897 older adults. Latent change score modelling (taking into account baseline cognitive level, chronic diseases, age, and central contributions to cognitive reserve through education, profession, and leisure engagement) revealed a significant interaction of frequency of Internet use and gender. More frequent Internet use in the first wave of data collection significantly predicted a smaller subsequent augmentation in TMT accomplishment time (i.e., a smaller subsequent cognitive decline) only in men, but not in women. In conclusion, frequent Internet use may contribute to the accumulation of cognitive reserve. The gender difference noted highlights an advantage for males. While this finding could be interpreted as gender-specific, it may be that the Internet activities males engage in differ from those of females, calling for a fine-grained investigation of Internet-based activities in future studies.
Use of biogenic silver nanoparticles in enhancing shelf life of Morus alba L. at post harvest stage
Authors: Das Dipayan , Mandal Palash 
Published: June 2020

AbstractMorus alba is one of the most important cultivated crop in Indian sub-continent contributing towards production of silk fibre that carries economic importance worldwide. Post harvest preservation of M. alba leaves is a challenging factor as decrease in concentration of essential metabolites that needed for silk gland development takes place. Decrease in chlorophyll, protein, sugar concentration and increase in accumulation of free radicals and ROS takes place at post harvest stage of preservation, putting negative impact on larval development indicated by high mortality rate. Silver nitrate and nanosilver solution acts as an effective preservative, enhances the activity of enzymatic and non-enzymatic antioxidants thereby reducing the harmful effect of accumulated free radicals and ROS. The effectiveness of nanosilver solution was found to be on the upper site without any significant difference than silver nitrate, as higher retention of primary metabolites like pigments, proteins, and sugar takes place. The impact of feeding nanosilver preserved leaves on silkworm was found on the positive trend as larval growth rate, cocoon weight, shell weight, effective rate of rearing was observed almost same to the larvae fed with fresh leaves.
Both metal-chelating and free radical-scavenging synthetic pentapeptides as efficient inhibitors of reactive oxygen species generation
Authors: Csire Gizella , Canabady-Rochelle Laetitia , Averlant-Petit Marie-Christine , Selmeczi Katalin , Stefan Loic 
Published: June 2020

Abstract
Reactive oxygen species (ROS) are major sources of oxidative stress playing prominent roles in the development of several pathologies including cardiovascular and neurodegenerative diseases or cancers. The presence of transition biometal ions, specifically copper and iron, induces ROS formation by catalyzing the reduction of molecular oxygen to superoxide anion (O2˙−), hydrogen peroxide (H2O2) and hydroxyl (HO˙) radical. To limit ROS production and their detrimental effects, we report on the synthesis, physicochemical studies and antioxidant assays of an innovative series of synthetic pentapeptides exhibiting a dual direct/indirect mode of action, both as iron(iii)-chelators and as radical scavengers. These combined effects lead to a drastic reduction of in vitro reactive oxygen species production up to 95% for the more reactive hydroxyl radical.
Development and validation of a nomogram for assessing survival in patients with hepatocellular carcinoma after hepatectomy
Authors: Huo Rong-Rui , Liu Xu , Cui Jing , Ma Liang , Huang Kun-Hua , He Cai-Yi , Yang Yang , You Xue-Mei , Yuan Wei-Ping , Xiang Bang-De , Zhong Jian-Hong , Li Le-Qun 
Published: June 2020

Abstract
Background and aim: Assessing the average survival rate of patients with hepatocellular carcinoma (HCC) after hepatectomy is important for making critical decisions in everyday clinical practice. The present study aims to develop and validate a nomogram for assessing the overall survival probability for such patients.
Methods: The putative prognostic indicators for constructing the nomogram were identified using multivariable Cox regression and model selection based on the Akaike information criterion. The nomogram was subjected to internal and external validation. The nomogram endpoints were death within 1, 3, and 5 years.
Results: A consecutive sample of 522 HCC patients who underwent potentially curative hepatectomy was retrospectively analyzed. Age, Barcelona clinic liver cancer (BCLC) stage, tumor size, alanine transaminase, alpha fetal protein, and serum prealbumin were included in the final model. The nomogram's discriminative ability was good in the training set (C-index was 0.74 for 1 year, 0.73 for 3 years, 0.70 for 5 years) and was validated using both an internal bootstrap method (C-index was 0.73 for 1 year, 0.72 for 3 years, 0.69 for 5 years) and an external validating set (C-index was 0.72 for 1 year, 0.72 for 3 years, 0.69 for 5 years). The calibration plots for the endpoints showed optimal agreement between the nomogram's assessment and actual observations.
Conclusions: The nomogram (an Excel-based tool) can be useful for assessing the probability of survival at 1, 3, and 5 years in patients with HCC after hepatectomy.
GPNMB contributes to a vicious circle for chronic obstructive pulmonary disease
Authors: Zhang Xi-Juan , Cui Zhong-Hua , Dong Yan , Liang Xiu-Wen , Zhao Yan-Xin , Baranova Ancha , Cao Hongbao , Wang Ling 
Published: June 2020

Abstract
Osteoporosis (OP) is significant and debilitating comorbidity of chronic obstructive pulmonary disease (COPD). We hypothesize that genetic variance identified with OP may also play roles in COPD. We have conducted a large-scale relation data analysis to explore the genes implicated with either OP or COPD, or both. Each gene linked to OP but not to COPD was further explored in a mega-analysis and partial mega-analysis of 15 independently collected COPD RNA expression datasets, followed by gene set enrichment analysis (GSEA) and literature-based pathway analysis to explore their functional linked to COPD. A multiple linear regression (MLR) model was built to study the possible influence of sample size, population region, and study date on the gene expression data in COPD. At the first step of the analysis, we have identified 918 genes associated with COPD, 581 with OP, and a significant overlap (P&lt;2.30e-140; 210 overlapped genes). Partial mega-analysis showed that, one OP gene, GPNMB presented significantly increased expression in COPD patients (P-value = 0.0018; log fold change = 0.83). GPNMB was enriched in multiple COPD pathways and plays roles as a gene hub formulating multiple vicious COPD pathways included gene MMP9 and MYC. GPNMB could be a novel gene that plays roles in both COPD and OP. Partial mega-analysis is valuable in identify case-specific genes for COPD.