bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hairy Root Culture an Alternative for Bioactive Compound Production from Medicinal Plants
Authors: Roy Arpita 
Published: December 2020

:
Medicinal plants produce a diverse group of phytocompounds like anthraquinones, alkaloids,
anthocyanins, flavonoids, saponins, and terpenes which are used in pharmaceutical, perfume,
cosmetics, dye and flavor industries. Commercial source of these metabolites is field-grown plants,
which are generally influenced by seasonal changes. Biotechnology possesses a significant role in production
of high-value secondary metabolites. By incorporating biotechnological methods, it is feasible
to manage biosynthetic pathways of the plant to enhance phytocompound production that is of pharmaceutical
interest. Plant cell suspension, shoot, adventitious root and hairy root culture are considered
as alternative methods for important bioactive compound production. These methods are controllable,
sustainable and overcome several inconveniences for large scale secondary metabolites production. At
present research on hairy root culture for valuable bioactive compound production has gained a lot of
attention. Agrobacterium rhizogenes is an agent which causes hairy root disease in a plant and this
leads to the neoplastic growth of root which is characterized by higher growth rate and genetic stability.
Various studies explore the hairy root culture for production of a wide range of bioactive compounds.
Scale-up of hairy root culture using bioreactors has provided an opportunity to enhance bioactive
compound production at the commercial level. The present review discusses the role of hairy root
culture in the production of valuable bioactive compounds, the effect of culture parameters on bioactive
compound production and bioreactor applications.
The Effect of Stress Management Program on Perceived Stress in Patients with Type 2 Diabetes: A Randomized Controlled Trial
Authors: Ehasanbakhsh Hadi , Mirzaei Amin , Bakhtiyari Salar , Zavare Mohammad Sadegh Abedzade , Jalilian Mohsen 
Published: December 2020

Introduction:
Stress management plays an important role in improving metabolic control in type 2 diabetes
patients. The aim of this study was to find the effect of educational intervention on improving stress management in type 2
diabetic patients in Dezful, Iran.

Methods:
In an experimental study, 92 patients with Type 2 Diabetes, who referred to the Diabetes Clinic of Ganjavian
Hospital of Dezful were selected by available sampling method. Then, they were randomly divided into two groups: 46 as
intervention and 46 as control. For the intervention group, a stress management training program was designed for one
month (8 sessions), while there was no training for the control group. All participants filled the Cohen Perceived Stress
Questionnaire (PSQ) in baseline and follow up (3 months) phases. All the data were analyzed using SPSS software by
conducting independent t- test, and paired sample t- test, and Chi-square test at a significant level of 0.05.

Results:
The mean age of participants was 52.70 ± 10.91 years. Pre-test data revealed that there was no significant
difference between the stresses of the two groups (P> 0.05); however, the results of the independent t-test, 3 months after
educational intervention, demonstrated a significant decrease in stress level in the intervention group compared to the
control group (P <0.05).

Conclusion:
Based on the findings of this study, it is concluded that the design and implementation of health education
interventions can be useful to improve the stress management in people with Type 2 Diabetes.
A Multicenter Prospective Hospital-based Cohort Study on the Efficacy and Safety of Pitavastatin
Authors: Shehab Abdullah , Elnour Asim Ahmed , Bhagavathula Akshaya Srikanth , Kurian Joseph Pulavelil , Hassan Gazi , AlZaabi Shareen , Gilbert Huburt , Al-Rasadi Khalid 
Published: December 2020

Aims:
We aim to investigate the efficacy and safety of pitavastatin 4 mg in a population
of people living in the United Arab Emirates (UAE).

Background:
Pitavastatin is a member of the HMG-CoA reductase inhibitors family which was approved
for use in adult subjects with primary hyperlipidemia or mixed dyslipidemia. To date, no
published studies have assessed the efficacy and safety of pitavastatin in the United Arab Emirates.

Objective:
The main objective of the current study was to investigate the efficacy and safety of pitavastatin
in subjects with dyslipidemia for the primary prevention of cardiovascular diseases based
on total cardiovascular risk.

Methods:
This was a multicentre (four private hospitals) prospective cohort study to analyze data
on the use of pitavastatin for dyslipidemia in adult outpatients in Abu Dhabi and Dubai, United
Arab Emirates. We have followed up the clinical profiles of subjects in four hospitals for six-weeks
during the period from June 2015 to June 2017. Efficacy was based on the evaluation of the mean
(± standard deviation) change in low-density lipoprotein cholesterol between baseline and week six
after the initiation of pitavastatin therapy. Safety was reported with respect to the incidence of adverse
events occurring with the use of pitavastatin and the development of new-onset diabetes.

Results:
A total of 400 subjects who were receiving pitavastatin 4 mg were included. The mean
age of subjects was 50.7 ±10.8 years; of these, 79.0% were males. At the baseline, the mean level
of total cholesterol was 185.4 ±41.5 mg/dL, low density lipoprotein was 154.9 ±48.55 mg/dL, high-
-density lipoprotein cholesterol was 40.5 ±11.23 mg/dL and fasting blood glucose was 115.0
(±16.63) mg/dl. At the end of six weeks, low density lipoprotein levels significantly decreased to
112.09 ±41.90 mg/dl (standard mean difference (SMD) (-42.8%), 95% CI: -42.88 [-49.17 to
-36.58] mg/dl, P <0.001), while high density lipoprotein levels improved (SMD, 95% CI: 1.77%
[0.25 to 3.28] mg/dl, P <0.022). There were 55 subjects (13.7%) who reported various adverse
events such as myalgia (7.5%), sleep disorders (2.5%), and myopathy (2.2%). Furthermore, 4
(1.0%) have had developed new-onset diabetes post-six-weeks of initiation of pitavastatin therapy.

Conclusion:
Pitavastatin 4 mg showed robust efficacy in reducing LDL-C levels and improving
HDL-C levels in subjects with dyslipidemia. The use of pitavastatin was associated with a low discontinuation
rate, fewer adverse events, and very limited cases of new-onset diabetes.
A Systematic Review on Pharmaceutical Diabetic Care Services in the United Arab Emirates (UAE)
Authors: Gillani Syed Wasif , Kaka Khan Khanda Kareem , Ladouani Douaa , Salama Nour Alaa 
Published: December 2020

Objective::
The aim of this systematic review study is to evaluate the current services and pharmaceutical care practices for diabetic patients in UAE.

Methods::
Systematic review coverage conforms to the guidelines of the PRISMA; the search was limited to primary research articles, which describe the term ‘’ pharmaceutical diabetes care services in UAE’’. The search strategy was developed in collaboration with a health database librarian and a predetermined protocol was developed in conjunction with the authors for search methods.

Results::
The findings showed the affect on a prescription treatment system on disease prevention and health related quality of life in patients with type 2 diabetes in United Arab Emirates. Implementing a retrospective interventional health evaluation to evaluate existing health procedure and the impact of conventional treatment on type 2 diabetes treatment (T2DM). The findings of this interventional evaluation were largely favorable, and the viability of changing the existing clinical procedure was stressed. The individualized strategy has helped clinicians reach a great result of terms of glycemic and BP, as well as patient satisfaction. The need of more work to clarify the long-term effect of organized strategy in enhancing the consistency of T2DM treatment in the UAE. The findings also showed increase community pharmacy services may further change the opinions of patients on the level of care provided from such pharmacies. The patients’ quality of life would improve by drug treatment efficacy and pharmacist services to mitigate diabetes complications.
The findings showed the effect on a prescription treatment system on disease prevention and health-related quality of life in patients with type 2 diabetes in the United Arab Emirates. Implementing a retrospective interventional health evaluation to evaluate existing health procedure and the impact of conventional treatment on type 2 diabetes treatment (T2DM). The findings of this interventional evaluation were largely favorable, and the viability of changing the existing clinical procedure was stressed. The individualized strategy has helped clinicians reach a great result of terms of glycemic and BP, as well as patient satisfaction. The need for more work to clarify the long-term effect of organized strategy in enhancing the consistency of T2DM treatment in the UAE. The findings also showed increase community pharmacy services may further change the opinions of patients on the level of care provided from such pharmacies. The patients’ quality of life would improve by drug treatment efficacy and pharmacist services to mitigate diabetes complications.

Conclusion::
This systematic review reported beneficial pharmacist-led diabetic management services in UAE. Several care strategies also highlighted to improve service for type 2 diabetes mellitus patients.
Refractory Behçet’s Disease with Multi-organ Involvement - Learning from Failure
Authors: Santos Christiana Sieiro , Morales Clara Moriano , Álvarez Elvira Díez , Sandoval Trinidad Pérez 
Published: December 2020

Background:
Behçet’s disease (BD) is a rare systemic vasculitis of unknown etiology
with relapsing and remitting course, characterized by triple-symptom complex of recurrent attacks
of oral aphthous ulcers, genital ulcers and ocular lesions and other clinical features that include dermatological,
cardiovascular, gastrointestinal, and neurological manifestations. The main goal of management
is to prevent relapses and suppress inflammation rapidly for major organ involvement
that may cause damage and even be fatal.

Case Presentation:
We hereby describe a case of a patient with Behçet’s disease followed in our
rheumatology consultation over the course of 15 years with multi-organ involvement resistant to
several treatments and showed partial results with IL-1 inhibitors, particularly anakinra and canakinumab,
while also discussing the current treatments of refractory BD and how early implementation
of treatments could make a difference.
An Overview of Phytochemical and Pharmacological Profile of Morus alba Linn
Authors: Paul Arpita , Rajiung Monami , Zaman Kamaruz , Chaudhary Sushil K. , Bhat Hans R. , Shakya Anshul 
Published: December 2020

Background:Morus alba Linn. commonly known as white mulberry, belongs to the family Moraceae, and is a promising traditional medicine. In Asia, besides its use in the preparation of delicacies, every part of this plant is utilized in traditional medicine. Over the past decade, studies related to the identification and isolation of biologically active compounds, with flavonoids as the major class of phytoconstituents, from this plant have been reported. These phytoconstituents are not only found to be beneficial for the maintenance of general health but also are associated with a range of potential pharmacological activities such as antioxidant, anti-inflammatory, anti- diabetic, anticancer, hepatoprotective, cardioprotective, neuroprotective to name a few.Objective:This review aims to provide upgraded and comprehensive information regarding the phytochemical, ethnomedicinal use and pharmacological profile of the plant Morus alba Linn.Method:The significant information has been collected through various databases viz. PubMed, Scopus, Web of Science, Science Direct based on the recent findings, using different terms of Morus alba.Results:The outcome of the study suggests that Morus alba is a multifunctional plant with numerous phytochemicals, and possesses a range of pharmacological activities.Conclusion:The data assembled on Morus alba will be beneficial to trigger research in various fields of pharmaceutical and allied sciences to explore the medicinal importance of this unique plant.
Phenolic Content, Antioxidant Properties, Key Enzyme Inhibitory Potential and Photoprotective Activity of Lawsonia inermis L.
Authors: Goudjil Rima , Mekhaldi Abdelkader , Benamar Houari , Bensouici Chawki , Kahoul Mohamed A. 
Published: December 2020

Background:
Lawsonia inermis Linn (Lythraceae), commonly known as "Henna" is a medicinal plant widely
used as a folk remedy and for its cosmetic properties.

Objective:
The objective of this present work was to evaluate biological activities and to quantify phenolics in extracts.

Methods:
The extracts were obtained from seeds of L. inermis by increasing the polarity of the solvent.
The content of total phenolics, flavonoids, flavonols and tannins was determined using colorimetric
methods. Also, to evaluate the antioxidant activity, six different assays, DPPH, ABTS, superoxide
radical scavenging, inhibition of β-carotene bleaching, ferric reducing antioxidant power
and phenanthroline assays were used. Enzyme inhibition activity was evaluated by acetylcholinesterase,
butyrylcholinesterase and tyrosinase inhibition assays. Furthermore, photoprotective activity
was determined by measuring sun protection factor.

Results:
The extraction efficiency of phenolics, as well as the biological activities of plant extracts,
were affected by solvent polarity. The highest content of phenolics was determined in methanol extract
(786.54 ± 0.00 mg GAEg-1 DW), followed by the aqueous and ethyl acetate extracts (526.48 ±
0.40 and 331.25 ± 0.00 GAEg-1 DW, respectively).The above extracts also exhibited the highest antioxidant
activity, while low polar extracts were characterized with the lowest content of phenolics,
as well as the lowest antioxidant capacity. The highest enzyme inhibition activities were found in
ethyl acetate extract. Moreover, the methanol extract showed the best photoprotective activity with
sun protection factor of 43.05 ± 0.37.

Conclusion:
These findings suggest a possible use of Henna seeds as a potential source of bioactive molecules with
antioxidant, enzyme inhibition and skin protection properties.
Selective and Sensitive Determination of Folic Acid Based on Molecularly Imprinted Poly (o-aminophenol) and Reduced Graphene Oxide Decorated with Au Nanoparticles
Authors: Peng Youyuan , Zhang Xiaojing 
Published: December 2020

Background:
Folic acid (pteroylglutamic acid, FA), known as a water soluble vitamin of B complex family,
plays an important role in the human body. However, excess intake of FA would mask the vitamin B12 deficiency
symptoms which may lead to other health risks. Therefore, it is very important to develop a method for the sensitive and
selective determination of FA in natural sources, fortified foods and multivitamin preparations.

Methods:
An electrochemical sensor was fabricated for the analysis of FA, which was based on
electropolymerized molecularly imprinted poly (o-aminophenol) film and reduced graphene oxide
decorated with Au nanoparticles composites (rGO-AuNPs). The transmission electron microscope,
cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry
were utilized for the characterization of the imprinted polymer film.

Results:
Under the optimized experimental conditions, the proposed sensor exhibited two distinct
linear responses ranging from 0.02 to 0.8 μmol L-1 and 0.8 to 10 μmol L-1 towards the concentrations
of FA, and the detection limit was found to be 2.8 nmol L-1 (S/N=3). The molecularly imprinted
film proposed was also found to exhibit comparatively high selectivity toward folic acid
against structurally similar analogues, and the preparation of the sensor was simple and reproducible.

Conclusions:
In this work, a molecularly imprinted film fabricated on an rGO-AuNPs modified
electrode was developed for the sensitive and selective determination of FA. Furthermore, the
method was applied to the detection of FA in infant formula milk, multivitamin tablets and blood
serum sample with satisfactory results.
New Method for Determination of Trans-resveratrol for Quality Evaluation of Red Wines by Multivariate Calibration Associated with UVVIS Spectroscopy
Authors: Mota Milleno D. , Meira Marilena , David Juceni P. , de Oliveira Ribeiro Erika M. , Brandão Hugo N. , do Vale Ademir E. , David Jorge M. 
Published: December 2020

Background:
This work describes a fast, simple, sensitive, and low-cost method for the
identification of resveratrol in different brands and varieties of red wines.

Methods:
It was developed based on a comparison of the UV-VIS spectra of the samples and samples
enriched with different concentrations of the trans-resveratrol standard. The spectra were analyzed
by chemometric principal component analysis (PCA) and multivariate calibration.

Results:
The PCA data indicated that only 4 main components made possible group samples based
on the grape variety characteristics and/or production region.

Conclusion:
From the construction of partial least squares (PLS) and multiple linear regression
(RLM) models, it was possible to predict the sample trans-resveratrol content with that sample
showing similarities between the groups observed in the PCA and the samples used in the model
constructions. The predicted trans-resveratrol present in these samples ranged from 0.29 to 23.3 mg L-1.
This multivariate method suggested a good predictive capacity of determination of resveratrol concentrations
in commercial red wines.
Use of Medications for the Treatment of Alcohol Dependence: A Retrospective Study Conducted in 2011-2012
Authors: Agabio Roberta , Balia Silvia , Gessa Gian Luigi , Pani Pier Paolo 
Published: December 2020

Background:
Pharmacotherapy for Alcohol Dependence (AD) is underutilized. Barriers
preventing the use of AD medications include high prices, lack of access to prescribing physicians,
and a limited number of available medications.

Objective:
The study evaluated the use of AD medications in a sample of Italian outpatients who received
these medications free of charge, had access to physicians during office hours, and for
whom substitution therapy [gamma-hydroxybutyrate (GHB)] was available. We also evaluated the
rate of patients who received a combination of non-pharmacological and pharmacological treatments
among participants who were still drinking.

Methods:
SCID for AD and questionnaire were filled by to AD outpatients during a face-to-face interview.

Results & Discussion:
345 AD outpatients were interviewed: 58.8% were currently receiving at
least one AD medication (GHB: 34.3%, disulfiram: 29.6%, acamprosate: 5.9%; naltrexone: 2.5%;
more than one medication: 16.7%). Less than 30% of participants who were still drinking, received
a combination of non-pharmacological and pharmacological treatments. Nonetheless, we found
higher use of AD medications compared to previous studies conducted in other countries. This
higher use of AD medications may be due to access to free medications, prescribing physicians’
style, and a larger number of available medications.

Conclusion:
Our results confirm the underutilization of AD medications, as less than 60% of AD
outpatients received medications, and less than 30% of those who were still drinking, received a
combination of non-pharmacological and pharmacological treatments. These findings may be useful
in improving our knowledge of the barriers that prevent the use of AD medications in clinical
practice.
Percursos metodológicos digitais aplicados na educação literária
Authors: Ribeiro Ana Luísa da Mota Antunes , Iriarte Ana Martins , Dias Inês de Jesus Amorim , Mendes Fátima Cabo , Gonçalves Nancy Amaral 
Published: December 2020

De forma a melhorar e a ajudar os alunos do 3.º ciclo do ensino básico na interpretação dos textos literários no Novo Programa e Metas Curriculares do Português, no domínio da Educação Literária, foi desenvolvida uma interface digital, constituída por dois percursos metodológicos digitais que auxiliam a análise de textos no período de pré-leitura. O primeiro percurso foca-se na escolha do livro opcional de leitura por parte do aluno, por um método de atividades de ligação, centrado na leitura e na conotação sentimental das sinopses e títulos das obras. O segundo percurso foca-se em ajudar na realização de uma atividade de análise de um excerto e consequente título, mediante a criação de nuvens de palavras. Esta interface digital ajuda os alunos na preparação do exame nacional do 9.º ano, como também na interpretação das obras e no incentivo à leitura através de um processo diversificado.
“You are Dead, but You are Not”: Social medium (Facebook) is the message in grieving and continuing bonds
Authors: Tan Huey Pyng 
Published: December 2020

Today, one may no longer alive but his social networking sites (SNS) account will still live on. Empirical studies on death and SNS started since 2004 covering issues on grief, bereavement, mourning, relational continuity functions of SNS, and digital legacy. Majority of them applied content and discourse analyses on SNS messages directed to or related to the deceased. Applying McLuhan’s aphorism medium is the message, researcher focused on the interplay among forms and functions of Facebook (FB) as a medium and message that mediates grief and the bereaved persons who are communicators that decided to grieve on FB. This research adopts grounded theory approach where in- depth interviews with 10 bereaved persons who maintain relational continuity with their deceased loved ones through FB were conducted. The conclusions are drawn to prove that bereaved users preferred to engage in a transcoporeal mediated communication with deceased for continuing bonds due to four main reasons – the deceased are perceived “dead but they are not”; maintaining relational continuity via FB is essential to “finish their unfinished business”; they felt aided and embraced when adopting “FB the Public Platform for Private Grief”, and they imagined a mutual communication based on “past experience and religious belief”. The mediated transcopereal communication (TcC) is enabled because the social medium (FB) is the message.
Exploring Private Finance Initiative Implementation Issues in Malaysia Construction Projects: A Way Forward
Authors: Lop Nor Suzila , Ismail Kharizam , Mohd Isa Haryati , Khalil Natasha 
Published: December 2020

Private Finance Initiative (PFI) was introduced in 2006 aimed at achieving value for money. Nevertheless, most of the PFI projects in Malaysia are currently facing difficulties in meeting the expectation. Therefore, a brainstorming workshop involving 69 PFI practitioners from diverse backgrounds was conducted to investigate the implementation issues at the operational level and strategies towards achieving the project goals. The results showed that skills and knowledge, procedures and implementation, challenges in project management, and conflict on documentation were among the issues debated among workshop participants. “Loopholes” were identified to facilitate the improvement of the PFI project implementation in Malaysia.
Keywords: Operational Performance; Private Finance Initiative (PFI); Issues; Strategies.
eISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bsby e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v5iSI3.2531