bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of CXCL8 as an Immune Microenvironment Regulator of Colorectal Cancer: A Bioinformatics Study Based on Cancer Genome Atlas and Gene Expression Omnibus Datasets
Authors: Shao Ying , Gao Shuhua , Cheng Minrong , Zheng Jinxiu , Yang Ting , Li Tie , Yang Lijun , Yang Tao 
Published: October 2020

Abstract
BackgroundAt present, a lack of knowledge of the molecular mechanisms underlying the genesis and progression of colorectal cancer (CRC) exists. This is one of the reasons for poor prognosis of this increasingly occurring disease. The purpose of our research is to identify molecular candidates for therapeutic intervention that would help stop the malignant progression of CRC.MethodsIn this study, we performed bioinformatic analysis of the combined microarray data GSE40967 and GSE8671 in Gene Expression Omnibus (GEO) and the CRC RNA-seq dataset in The Cancer Genome Atlas (TCGA). This analysis consisted of 1067 CRC samples and 92 normal mucosae in total. We identified the common differentially expressed genes (DEGs) using R and Venn software. Protein–protein interaction (PPI) network information was obtained using the Search Tool for the Retrieval of Interacting Genes (STRING) Database. The Cytoscape plug-ins MCODE and cytoHubba were used to screen hub modules. CXCL8, which was the selected hub gene, was subjected to functional analysis and Gene Set Enrichment Analysis (GSEA). The algorithm “Cell type Identification By Estimating Relative Subsets Of RNA Transcripts (CIBERSORT)” was employed to estimate the relative abundance of tumor infiltrating lymphocyte cells (TILs) with CXCL8 expression. Finally, correlation analysis was carried out to explore the underlying mechanisms.ResultsWe identified 135 common DEGs, which consisted of 38 upregulated genes and 97 downregulated genes. The upregulated DEGs were strikingly enriched in regulation of neutrophil chemotaxis and cytokine-cytokine receptor interaction. The downregulated DEGs were enriched in bicarbonate transport. We identified the candidate molecule CXCL8 as a product of the hub gene. Functional enrichment analysis and GSEA indicated that CXCL8 may be closely related to a regulator of the CRC immune microenvironment. Finally, TILs analysis and correlation analysis showed that CXCL8 may inhibit CD8+ T cell immune infiltration through the HIF-1α/PD-Ls axis. In addition, CXCL8 could induce the polarization of M2 macrophages through the PI3K/AKT3 pathway.ConclusionsWe determined that hub gene CXCL8 may promote immune escape of CRC by inhibiting CD8+ T cell immune infiltration or promoting polarization of M2 macrophages. CXCL8 is a potential therapeutic target for the treatment of CRC patients.
Downregulation of Urinary microRNA-200c acts as a Promising Novel Bio-marker in the Diagnosis of Patients With Bladder Cancer
Authors: Zi Hao , Tao Wen-Lin , Gao Lei , Yu Zhao-Hua , Bai Xiao-Dong , Li Xiao-Dong 
Published: October 2020

Abstract
BackgroundMicroRANs (miRNAs) have been reported to be involved in various human cancers. The aim of this study was to explore the diagnostic performance of urine miR-200c in bladder cancer. MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) method was applied to measure the relative expression of urine miR-200c in bladder cancer patients. The relationship between urine miR-200c level and clinicopathological factors was analyzed using χ2 test. The diagnostic capacity of urine miR-200c was calculated using the receiver operating characteristics (ROC) curve analysis.ResultsUrinary level of miR-200c was significantly reduced in bladder cancer patients compared with healthy controls (P=0.000). Furthermore, urine miR-200c expression was strongly correlated with histologic grade (P=0.019), tumor grade (P=0.003), and lymph node metastasis (P=0.001). ROC curve showed that urine miR-200c could distinguish bladder cancer patients from healthy controls with an area under the curve of 0.844. The cutoff value of 1.235, with the sensitivity of 89.0% and the specificity of 70.7% respectively.ConclusionUrine miR-200c may act as a noninvasive diagnostic biomarker for bladder cancer.
Rare Huge Epidural Hematoma Associated with Re-fracture of Kummell Disease: A Case Report and Literature Review
Authors: Bai Lu Lu , Wang Wen Tao , Xue Xu Kai , Du Jin Peng , Hao Ding Jun , Wang Jian Feng , Ge Chao Yuan , Cao Dong Zi , Lu Liang 
Published: October 2020

Abstract
Background Kummell disease combined with huge spinal epidural hematoma is a very rare phenomenon, and its potential pathogenesis and natural course remain unclear.Case description We describe a rare case of Kummell disease with huge spinal epidural hematoma. A 75-year-old male was diagnosed with osteoporotic vertebral compression fractures and was treated conservatively. After suffering minor trauma again 8 days ago, he presented unbearable low back pain and activity restriction. Lumbar MRI showed that L1 vertebral had re-fracture and intervertebral vacuum cleft, and a huge spinal epidural hematoma extending from T12- L1. Due to the patient had no neurological deficits and unbearable low back pain, percutaneous vertebroplasty was performed, and pain was relieved significantly. The follow-up MRI showed that the hematoma almost disappeared 7 days after the operation.Conclusion Although extremely rare, there is a possibility of spinal epidural hematoma after re-fracture of Kummell disease, and it could be further confirmed via MRI and pathological examination. Timely operation is recommended, and the results are usually favorable.
Comparison of Growth in Children 6 to 59 Months of Age According to Birth Order: Insights From the National Family Health Survey-4 India
Authors: Ghimire Ananta , Dharmaraj Aravind 
Published: October 2020

Abstract
Background Undernutrition continues to be a major public health problem throughout the world. The present study aimed to understand the prevalence and determinants of undernutrition in India and determine what extent it differs by birth order, other child level, maternal and socioeconomic factors.MethodsThis study used information from a total weighted sample of 128859 mothers from India National Family Health Survey 4. Univariate and Multivariate logistic regression were used to investigate the association between undernutrition with birth order, other child level, maternal and socio-economic factors. Three models were constructed for the study, model 1 as univariate, model 2 adjusting with birth order and socioeconomic predictors and model 3 adjusting with all the predictors included in the study. Results The prevalence of stunting, underweight and wasting were 37.9, 34.0 and 20.7 respectively. The mean age of children was 2.4 years (standard deviation 1.3) of which majority were second order birth (33.6%), males (54.5%), anemic (58.9%) and normal birth weight (87.2%). All three models showed that higher birth order was a strong predictor of a child being stunted and underweight after adjusting for potential confounders. Children with lower wealth quintiles, anemia, male, low birth weight and vaginal delivery had higher odds of being stunted, wasted and underweight in model-3 adjusted analysis.Conclusion The findings from this study provide an important interaction between birth order and child undernutrition status in India. However, further longitudinal studies are required to establish such cause-effect relationship between birth order and undernutrition.
Satisfaction in Delivering Maternal and Neonatal Health Services During a Pandemic: Recommendations for Infection Preparedness and Response Protocols and Employee Support for Community Health Workers&nbsp;
Authors: Jafree Sara Rizvi , Momina Ain ul , Mahmood Qaisar Khalid , Khawar Amna 
Published: October 2020

Abstract
Aim: Community health workers (CHWs) have emerged as salient health team members in disadvantaged primary settings to provide critical services to disadvantaged mothers and their newborns. There is need for empirical evidence to understand how CHWs may be supported in delivering maternal and neonatal health services during pandemics. Subject and Methods: In this study we used bivariate regression to identify the lower odds for CHW perceived satisfaction for maternal and neonatal health services, with respect to client socio-demographic characteristics, coronavirus preparedness, coronavirus responsiveness, and employee satisfaction. In addition, we used structural equation modeling to investigate if coronavirus responsiveness and employee satisfaction as mediating variables influence the relationship between coronavirus preparedness and maternal and neonatal health services. Results: From a sample of 350 CHWs across 35 districts of Punjab, we found 30 predictors with respect to coronavirus preparedness, coronavirus responsiveness and employee satisfaction which contribute to lower odds of maternal and neonatal health services. We also identified that employee satisfaction is a key mediator in the relationship between coronavirus preparedness and maternal and neonatal health services. Conclusion: We conclude with 4 critical recommendations to support CMWs in delivering optimal services, comprising: education and training, operational support, public acceptance, and employee support and benefits.
NFATc1 Promotes Epithelial-Mesenchymal Transition and Facilitates Colorectal Cancer Metastasis by Targeting SNAI1
Authors: Shen Tianli , Yue Chenyang , Wang Xingjie , Wang Zijun , Wu Yunhua , Xiao Qian , Zhao Chenye , Qu Chao , Ye Changchun , Tian Qi , Sun Xuejun , WANG WEI 
Published: October 2020

Abstract
BackgroundMetastatic recurrence remains a major cause of colorectal cancer (CRC) mortality. In this study, we focused on the role and the potential underlying mechanisms of nuclear factor of activated T cells 1 (NFATc1) in CRC metastasis. MethodsWe examined the expression of NFATc1 in 140 cases of CRC tissues and 35 corresponding adjacent tissues, as well as analyzed the correlation between NFATc1 expression levels and clinical stages. The role of NFATc1 in CRC metastasis and the molecular mechanisms were investigated in both in vitro and in vivo models. ResultsThe results showed that NFATc1 expression was increased in metastatic CRC tissues and positively associated with clinical stages (Stage I vs. Stage II, III or IV) of CRC. Overexpression of NFATc1 promoted CRC cell migration, invasion and epithelial-mesenchymal transition (EMT). Moreover, SNAI1 was verified as the direct transcriptional target of NFATc1 and interacted with Slug to promote EMT. Remarkably, our lung and liver double metastasis mouse model demonstrated that NFATc1 overexpression accelerated CRC metastasis, and treatment with FK506, a calcineurin-NFAT pathway inhibitor, could suppress CRC metastasis in vivo. ConclusionsTaken together, our findings suggest that NFATc1 could transcriptionally activate SNAI1, which in turn could interact with Slug to mediate EMT and to promote CRC metastasis, making NFATc1 a promising target in CRC treatment.
Background splicing and genetic disease
Authors: Alexieva Diana , Long Yi , Sarkar Rupa , Dhayan Hansraj , Bruet Emmanuel , Winston Robert , Vorechovsky Igor , Castellano Leandro , Dibb Nick 
Published: October 2020

Abstract
We report that low level background splicing by normal genes can be used to predict the likely effect of splicing mutations upon cryptic splice site activation and exon skipping, with emphasis on the DBASS databases, BRCA1, BRCA2 and DMD. In addition we show that background RNA splice sites are also involved in pseudoexon formation, recursive splicing and aberrant splicing in cancer. We discuss how background splicing information might inform splicing therapy.
Assessment of the changes in the stress related salivary cortisols levels induced during different Dental procedures
Authors: Phukela Sumit Singh , Ritwal Pankaj , Dhall Rupinder Singh , Yadav Pooja , Madan Reshu , Yadav Jaiveer 
Published: October 2020

Aims and objective: Anxiety ,fear and pain assessment is essential in day to day Dental practice .The present study evaluated the stress related salivary cortisols levels induced during scaling,tooth preparation and surgical flap elevation in Periodontally compromised subjects
Methodology: The salivary cortisol level were assessed in 80 patients ,out of which 40 patients were healthy patient who do not require any Dental treatment and 40 patients who underwent Scaling , tooth preparation and surgical flap elevation in Periodontally compromised subjects
The groups divided were as follows .
Group 1: control group with no treatment and just the saliva sample was taken.
Group 2; The patients underwent scaling
Group 3; The patients underwent tooth preparation for fixed prosthesis
Group 4; The patients underwent surgical flap elevation in Periodontally compromised situation .The salivary cortisol levels were assessed using Salivary Cortisol Enzyme Immunoassay Kit.
Results: The salivary cortisol level was highest in Group 4 followed by Group 2, Group 3 and least in Group 1.
Conclusion: The stress level was lowest in healthy patient and highest when patient underwent surgical flap elevation in Periodontally compromised subjects .Dentist should try to minimize subject anxiety and stress to great extent .
Remineralization potential of GC Tooth Mousse and GC Tooth Mousse plus on initial caries like lesion of primary Teeth – An in-vitro comparative evaluation
Authors: Anshul  , Kishor  Jha Kaushal 
Published: October 2020

Aim: Teeth are constantly going through cycles of demineralization and remineralization. The ultimate goal of clinical intervention is the preservation of tooth structure and the prevention of lesion progression to the point where restoration is required. Thus promoting remineralization is the ultimate goal of clinical prevention of caries lesion. The present in vitro study aimed to investigate the efficacy of GC Tooth Mousse (CPP-ACP) and GC Tooth Mousse Plus (CPP-ACP)F on artificial enamel caries in primary human teeth.

Methods and Material: Sixty freshly extracted human primary anterior teeth were used in this study.
The root portion of 60 primary anterior teeth was separated from the crown portion at the cemento-enamel junction (CEJ)
Teeth samples were divided into 3 Groups (n=20 each). Group 1 as a control group, Group 2 GC Tooth Mousse, and Group 3 Tooth Mousse Plus containing dentifrices were used. Samples were subjected to 10 days of pH cycling protocol.
The changes were analyzed using Vickers Hardness Testing Machine and SEM.

Pre and post groups were compared by paired t-test. Independent groups were compared by one-way analysis of variance.

Result:
Micro-morphological observations of the enamel surfaces with SEM :
Group 1 the enamel scanning showed shallow depressions and fine porosities within these depressions, Group 2 showed numerous granular particles and amorphous crystals which were arranged on the enamel surface. Smooth, homogeneous surface, and no irregularities were seen in Group 3.
Surface Microhardness Evaluation
After treatment, the mean hardness Group III was the highest followed by Group II and Group I (i.e. Group I < Group II < Group III).

Conclusion: GC Tooth Mousse Plus showed a statistically significant amount of remineralization.
Perceived sources of stress amongst Indian dental students in Bareilly city
Authors: Saleem Adeeba , Gupta Puneet , K  K Shivalingesh , Mir Henna , Srivastav Divya , Singh Aditya  Veer 
Published: October 2020

Introduction: In addition to the stresses pertaining to dentistry as a profession, dental students have to face the additional stress of their studies. Through stress can also contribute to decreased student performance. The aim of this study was to assess perceived sources of stress among undergraduate dentists at a Dental College in Bareilly, India.
Materials and methods: A modified dental environment stress (DES) questionnaire which consisted of 38 questions was used to assess the levels of stress.
Results: The first major stressor for all the students was examination and grades followed by full working day, fear of unemployment after graduation, receiving criticism from supervisors about academic or clinical work, amount of cheating in dental faculty, rules and regulations of the faculty. Fear of unemployment after graduation and lack of relaxation were among the six highest stressors in each year, at least four. The perception of stress between genders with a male predilection was significantly different. Eighteen of the 38 questionnaire items had significant differences across the year groups including clinical DES items.
Conclusion: The primary sources of stress perceived by dental students were exams and grades followed by full working day and receiving criticism about academic or clinical work from superiors. Since high levels of perceived stress can reduce student performance, dental schools should consider courses in stress management.
Commentary: How to do personal network surveys: from name generators to statistical modeling
Authors: Maya-Jariego Isidro 
Published: October 2020

Abstract
The book “Conducting Personal Network Research” is a conceptual and methodological introduction to the structural study of personal networks. It is part of a series of recent monographs that have begun to systematize the knowledge generated in this area in recent decades (Crossley et al., 2015; McCarty et al., 2019; Perry et al., 2018). In this case, the authors have dedicated a large part of their career to the empirical investigation of the interpersonal relationships, interaction contexts, and social integration processes of immigrants, along with other groups in vulnerable situations. With this publication, all this experience is now reflected in a clear and comprehensive introductory text. This book explains how to integrate relational data collection and analysis with survey research. It systematically presents the strategies to estimate the size of personal networks. Finally, it describes how to fit statistical analysis to relational data, including regression models, multi-level models, and longitudinal models.
Deformation and strength of mantle relevant garnets: Implications for the subduction of basaltic-rich crust
Authors: Vennari Cara E. , Lin Feng , Kunz Martin , Akaogi Masaki , Miyagi Lowell , Williams Quentin 
Published: October 2020

Abstract
Garnet is an important mineral phase in the upper mantle as it is both a key component in bulk mantle rocks, and a primary phase at high pressure within subducted basalt. Here, we focus on the strength of garnet and the texture that develops within garnet during accommodation of differential deformational strain. We use X-ray diffraction in a radial geometry to analyze texture development in situ in three garnet compositions under pressure at 300 K: a natural garnet (Prp60Alm37) to 30 GPa, and two synthetic majorite-bearing compositions (Prp59Maj41 and Prp42Maj58) to 44 GPa. All three garnets develop a modest (100) texture at elevated pressure under axial compression. Elasto-visco-plastic self-consistent (EVPSC) modeling suggests that two slip systems are active in the three garnet compositions at all pressures studied: {110}&lt;111&gt; and {001}&lt;110&gt;. We determine a flow strength of ~5 GPa at pressures between 10 to 15 GPa for all three garnets; these values are higher than previously reported yield strengths measured on natural and majoritic garnets. Strengths calculated using the experimental lattice strain differ from the strength generated from those calculated using EVPSC. Prp67Alm33, Prp59Maj41, and Prp42Maj58 are of comparable strength to each other at room temperature, which indicates that majorite substitution does not greatly affect the strength of garnets. Additionally, all three garnets are of similar strength as lower mantle phases such as bridgmanite and ferropericlase, suggesting that garnet may not be notably stronger than the surrounding lower mantle/deep upper mantle phases at the base of the upper mantle.
Effect of magnesium on monohydrocalcite formation and unit-cell parameters
Authors: Vereshchagin Oleg S. , Frank-Kamenetskaya Olga V. , Kuz’mina Maria A. , Chernyshova Irina A. , Shilovskikh Vladimir V. 
Published: October 2020

Abstract
Monohydrocalcite (MHC) is hydrated calcium carbonate, which plays an active role in many geological processes, carbonate biomineralization, and can be used for fundamental science (as a paleoenvironmental indicator) and industry (for removal of hazardous anions). Despite a great number of works, the conditions preferable for MHC formation/stabilization and MHC crystal chemical patterns in relation to Mg and H2O are not clarified yet. In the course of current work, we conducted 38 syntheses to obtain information on MHC formation at different Mg/Ca ratios (0–12), pH (~9–12), and temperature (23 and 3 °C). Newly formed carbonate precipitates were studied by means of X-ray powder diffraction, optical, and scanning electron microscopy, energy-dispersive X-ray spectroscopy, Raman, and Fourier-transform infrared (FTIR) spectroscopies. The phase diagram for MHC, calcite, aragonite, and dypingite as a function of pH and Mg concentration in solution at T = 23 °C and Ca/CO3 = 0.5 was obtained. We demonstrated that MHC could be stable in dry conditions for up to two years and that the time of crystallization is important for the transformation of amorphous calcium carbonate to MHC. Our results on synthetic MHC stability show that the widespread idea that MHC is a short-lived intermediate phase is wrong. For the first time, on the basis of a regular changes in the unit-cell parameters the possibility of significant incorporation of magnesium in MHC has been demonstrated. According to FTIR data, it is shown to be accompanied by an increase in the water content, which leads to multidirectional change in a and c MHC parameters.
Water, sanitation, and hygiene (WASH) conditions and prevalence of office visits due to anemia: a regional-level analysis from Peru
Authors: Mougenot Benoit , Amaya Elard , Herrera-Añazco Percy 
Published: October 2020

AbstractIn Peru, anemia affects around 30% of children under the age of four, making it one of the major public health problems of the country. The literature suggests that water, sanitation, and hygiene (WASH) conditions may reduce anemia prevalence; however, empirical evidence of this association remains scarce for the Peruvian context. The objective of this study was to analyze the association between WASH conditions and office visits due to anemia (OVA), at the regional level during the years 2010–2018. Using generalized estimating equation models (GEE), we find that the prevalence of OVA increases by 0.24/1,000 per one percentage point (PP) increase in the proportion of households with access to safe drinking water and decreases by 0.22/1,000 per one PP increase in the proportion of households with basic sanitation. Our results suggest that policymakers should not only ensure a better coverage of drinking water sources but also ensure adequate quality.
Transitional Forms of Universal Basic Income and Options of Testing Tools in Russia, Illustrated by Unemployed
Authors: Bobkov V. N. , Antipov V. I. , Kolmakov I. B. , Pavlova V. V. 
Published: October 2020

Problems in researching the category (universalbasicincome – UBI) were found in a number of publications. Taking into account the idea of the inevitable introduction of UBI system it is necessary to resolve problems in researching options of such systems’ functioning, such as: what can be the source of the income, what can be the UBI value, customer number and therefore, the amount of state expenses. Russia has not got answers to these questions. The article studies problems of introducing models of UBI transitional forms or the so-called conditional basic income (conditionalbasicincome – CBI), having in mind target categories of recipients, limited payment time and its small amount compared with national living minimum. This approach could allow us to develop methodology of calculating options of future UBI systems. The authors substantiate and estimate the concrete model of paying the conditional basic income for the category of unemployed Russian citizens registered in employment centers