bluuebunny/crossref_metadata_2025_split huggingface dataset:
Registration of Spear‐shaped Leaf peanut genetic stock
Authors: Brown Nino , Branch William D. 
Published: June 2020

AbstractIn 2012, a single Spear‐shaped Leaf mutant peanut (Reg. no. GS‐11, PI 693219) (Arachis hypogaea L.) plant was identified in a breeding trial plot of ‘Georgia‐06G’ at the University of Georgia, Coastal Plain Experiment Station's Gibbs Research Farm in Tifton, GA. It was believed to be a spontaneous mutation; an inheritance study examining segregation in an F2 population concluded single gene inheritance with incomplete dominance, and the gene was designated SpL. The unique phenotype and inheritance of this mutation makes the Spear‐shaped Leaf an interesting mutant to be preserved as a genetic stock and released to the public for researchers interested in the genetic, biochemical, and physiological aspects of leaf shape and leaf development. The Spear‐shaped Leaf mutant could also serve as a tool to measure outcrossing in the field or as a border or plot marker.
Influence of dielectric barrier discharge cold plasma on physicochemical property of milk for sterilization
Authors: Wu Xiaomeng , Luo Yanghe , Zhao Fujunzhu , M Safian Murad , Mu Guangqing 
Published: June 2020

AbstractThe aim of this study was to evaluate the mechanism of dielectric barrier discharge cold plasma (DBD‐CP) sterilizing function on milk and to analyze the influences of DBD‐CP on the structure and physicochemical performance of milk. It was verified that DBD‐CP sterilizing function resulted in a broken bacterial cell membrane, reduced activity of metabolic enzymes, and bacterial DNA destruction. DBD‐CP treatment would bring in some more stabilized structures obtained from scanning electron microscopy and Fourier‐transform infrared spectroscopy. The values of color, viscosity, pH, and titratable acidity showed an acceptable physicochemical property with DBD‐CP treatment at 70 V (120 s) and 80 V (120 s). Therefore, it indicated that DBD‐CP could be used as a new sterilization method for milk.
Metabolism and disposition of oseltamivir (OS) in rats, determined by immunohistochemistry with monospecific antibody for OS or its active metabolite oseltamivir carboxylate (OC): A possibility of transporters dividing the drugs’ excretion into the bile and kidney
Authors: Fujiwara Kunio , Yamamoto Yutaro , Saita Tetsuya , Matsufuji Senya 
Published: June 2020

AbstractAmong any drugs, no comparative pharmacological study on how prodrug and its active metabolite behave in animal bodies is available. Immunohistochemistry (IHCs) using newly prepared two monoclonal antibodies, AOS‐96 and AOC‐160, monospecific for oseltamivir (OS) and its metabolite oseltamivir carboxylate (OC) were developed, simultaneously detecting the uptake or excretion of OS and OC in the intestine, liver, and kidney of rats to which OS was orally administered. In the intestine, IHC for OS revealed OS highly distributed to the absorptive epithelia with heavily stained cytoplasmic small granules (CSGs). IHC for OC showed that OC also distributed highly in the epithelia, but without CSGs, suggesting that OS was partly converted to OC in the cells. In the liver, OS distributed in the hepatocytes and on their bile capillaries, as well as on the lumina from the bile capillaries to the interlobular bile ducts. OC distributed in the whole cell of the hepatocytes, but without CSGs nor on any lumina through the interlobular bile ducts. In the kidney, a few levels of OS distributed in the cytoplasm of almost all the renal tubule cells, but they contained numerous CSGs. In contrast, OC distributed highly in the proximal tubules, but very slightly in the lower renal tubules of the nephrons. Thus, it was concluded that the two drugs behave in completely different ways in rat bodies. This paper also discusses a possibility of the correlation of OS or OC levels in tissue cells with their known transporters.
Fourier transform ion cyclotron resonance mass spectrometry for the analysis of molecular composition and batch‐to‐batch consistency of plant‐derived polyphenolic ligands developed for biomedical application
Authors: Zherebker Alexander Ya. , Rukhovich Gleb D. , Kharybin Oleg N. , Fedoros Elena I. , Perminova Irina V. , Nikolaev Evgeny N. 
Published: June 2020

RationaleComplex plant‐derived polyphenols are promising for biomedical application. Their high complexity prevents the use of conventional pharmacopoeia techniques to perform quality control. The goal of this study was to apply ultra‐high‐resolution mass spectrometry to evaluate the batch‐to‐batch consistency of the molecular composition of a polyphenolic ligand using appropriate statistical metrics.MethodsPolyphenols were obtained by hydrolyzed‐lignin oxidation. Manufacturing was performed under a range of reaction conditions: heating cycles, oxygen flows, purification. Direct‐injection Fourier transform ion cyclotron resonance mass spectrometry (DI FTICR‐MS) was applied to analyze reaction products. For pairwise comparison Jaccard and Tanimoto similarities calculations were proposed. In addition, principal component analysis (PCA) was applied for sample grouping based on the molecular class contributions.ResultsFTICR‐MS analysis revealed moderate Jaccard similarity of products synthesized under the same conditions, which shared about 50% of the formulae calculated in each sample. The intensity‐based Tanimoto index indicated high similarity of major components distribution of samples synthesized under standard conditions, while products obtained with variations in synthetic conditions were significantly different. PCA of molecular class contributions showed similar grouping with a high cumulative score.ConclusionsFTICR‐MS provides robust metrics for the examination of batch‐to‐batch consistency of synthetic polyphenol materials. This approach can be proposed for the analysis of reference samples and for development of complementary methods for quality control of medicinal agents based on various biologically active matrices.
Legume silvopastoral systems enhance soil organic matter quality in a subhumid tropical environment
Authors: Lira Junior Mario Andrade , Fracetto Felipe José Cury , Ferreira Juscélia da Silva , Silva Mariana Brito , Fracetto Giselle Gomes Monteiro 
Published: June 2020

AbstractThe silvopastoral system avoids the degradation of tropical grasslands by providing essential nutrients to the soil while improving livestock diet and farmer's income by increasing the marketable wood supply. We hypothesize that intercropping shrub‐tree legumes into signalgrass increases microbial activity and soil organic matter (SOM) quality. We investigated silvopastoral system soils over 5 yr in a subhumid climate in northeastern Brazil to determine the total C and N contents and to estimate C‐microbial biomass and respiration, microbial and metabolic quotients, humic acid (HA), and fulvic acid (FA). The experimental design evaluated (a) signalgrass intercropped with gliricidia at 0, 4, and 8 m from the center of the double legume row; (b) signalgrass intercropped with sabia at 0, 4, and 8 m from the center of the double legume row; and (c) single signalgrass. Samples were obtained after 2, 3, 4, and 5 yr from silvopastoral system implementation. The shrub‐tree legumes soils had total C (39 g kg−1) and total N (3.8 g kg−1) contents significantly higher (30 and 72%, respectively) than single signalgrass soil only on the fifth year after establishment. Microbial biomass C at 0 and 4 m from the legume's rows was 700 mg kg−1, which was 75% higher than single signalgrass soils. There was strong microbial respiration (∼50 mg C‐CO2 kg−1 d−1) in legumes soils, but the metabolic quotient did not indicate disturbance in microbial activity. The microbial quotients were significantly higher in shrub‐tree legumes soil, and the HA/FA ratio was nearly 1, demonstrating greater efficiency in SOM decomposition by microbial activity. Overall, 5 yr after establishment, the silvopastoral system has improved SOM quality and contributed to grassland sustainability.
Randomization‐based interval estimation in randomized clinical trials
Authors: Wang Yanying , Rosenberger William F. 
Published: June 2020

AbstractRandomization‐based interval estimation takes into account the particular randomization procedure in the analysis and preserves the confidence level even in the presence of heterogeneity. It is distinguished from population‐based confidence intervals with respect to three aspects: definition, computation, and interpretation. The article contributes to the discussion of how to construct a confidence interval for a treatment difference from randomization tests when analyzing data from randomized clinical trials. The discussion covers (i) the definition of a confidence interval for a treatment difference in randomization‐based inference, (ii) computational algorithms for efficiently approximating the endpoints of an interval, and (iii) evaluation of statistical properties (ie, coverage probability and interval length) of randomization‐based and population‐based confidence intervals under a selected set of randomization procedures when assuming heterogeneity in patient outcomes. The method is illustrated with a case study.
New M5P model tree‐based control for doubly fed induction generator in wind energy conversion system
Authors: Ali Mounira , Talha Abdelaziz , Berkouk El madjid 
Published: June 2020

AbstractThis paper presents a new algorithm based on the combination of fuzzy logic control with M5 decision tree algorithm for doubly fed induction generator active and reactive power control. The M5 model tree is a machine learning algorithm that uses the extracted dataset from fuzzy logic for the aim of control performance enhancement. Because the fuzzy logic provides high design and implementation complexity, the new control approach is proposed to reduce its complexity and to achieve fast dynamic control by translating the fuzzy logic algorithm into simple if‐then instructions using M5 model tree. The obtained model shows good agreement with fuzzy logic in rotor side control. The effectiveness of the proposed control approach is investigated through Matlab/Simulink software. Furthermore, a cosimulated through processor in the loop testing is performed as an experimental verification using a low‐cost STM32F407 discovery board.
Safe Use of Butorphanol–Azaperone–Medetomidine to Immobilize Free‐Ranging White‐tailed Deer
Authors: McDermott Joseph R. , Leuenberger Wendy , Haymes Caleb A. , Clevinger Garrett B. , Trudeau Jonathan K. , Carter Tim C. , Hast John T. , Jenkins Gabriel S. W. , Bowling Will E. , Cox John J. 
Published: June 2020

ABSTRACTButorphanol–Azaperone–Medetomidine (BAM) is a relatively new drug mixture compounded for the past decade to immobilize mammals, particularly ungulates. Despite its increased use in recent years, scant research has quantified the physiologic responses of immobilized animals or assessed its relative efficacy using different trapping methods. We tested the safety and efficacy of BAM for use in the immobilization of 198 free‐ranging white‐tailed deer (Odocoileus virginianus) captured using drop‐nets, Clover traps, and gun‐propelled darts from 1 January 2014 to 28 July 2016 in Kentucky and Indiana, USA. Use of BAM produced a safe and satisfactory plane of immobilization that rarely required treatment of side effects. First signs of induction were observed on average at 4.4 ± 0.2 (standard error) minutes post–intramuscular administration, and deer reached lateral recumbency at 8.6 ± 0.4 minutes. Times to first signs of induction and the induction period were longer for adults than for juveniles, while times to the first sign of reversal, lifting head, and standing were longer for juveniles than adults. Although physiologic responses of deer during induction were within published norms, respiration rates, body temperature, heart rates, and oxygen saturation typically declined throughout the immobilization period. Our BAM dose did not affect time to recovery or heart rate. Regardless of trapping method, on average, heart rate was 61.2 ± 0.7 beats/minute, respiratory rate was 29.1 ± 0.5 breaths/minute, temperature was 38.93° ± 0.04° C, and oxygen saturation was 85.0% ± 0.3%. Deer showed first signs of reversal at 4.3 ± 0.3 minutes after administration of the reversal agent intramuscularly or half‐intramuscularly and half‐intravenously and were fully recovered after 6.3 ± 0.4 minutes. In summary, we found that BAM was an efficacious and safe drug to use on white‐tailed deer captured by a variety of trapping methods. © 2020 The Wildlife Society.
SIMPLE BOUNDED WEIGHT MODULES OF $$ \mathfrak{sl}\left(\infty \right),\kern0.5em \mathfrak{o}\left(\infty \right),\kern0.5em \mathfrak{sp}\left(\infty \right) $$
Authors: GRANTCHAROV DIMITAR , PENKOV IVAN 
Published: June 2020

AbstractWe classify the simple bounded weight modules of the Lie algebras$$ \mathfrak{sl}\left(\infty \right),\kern0.5em \mathfrak{o}\left(\infty \right) $$sl∞,o∞and$$ \mathfrak{sp}\left(\infty \right) $$sp∞, and compute their annihilators in$$ U\left(\mathfrak{sl}\left(\infty \right)\right),\kern0.5em U\left(\mathfrak{o}\left(\infty \right)\right),\kern0.5em U\left(\mathfrak{sp}\left(\infty \right)\right) $$Usl∞,Uo∞,Usp∞, respectively.
Ursachen und Auswirkungen von Gewalt in der Notfallmedizin
Authors: Lindner T. , Weissenberger G. , Hottenbacher L. , Möckel M. 
Published: June 2020

ZusammenfassungMitarbeiter*innen in der präklinischen und klinischen Notfallmedizin sind regelmäßig mit gewalttätigem Verhalten konfrontiert. Auch wenn Definitionen und Studiendesigns sehr heterogen sind, handelt es sich um ein relativ gut im Ausmaß beschriebenes internationales Problem. Viele Mitarbeiter*innen akzeptieren gemachte Gewalterlebnisse, welche überwiegend verbaler Natur sind, als „Teil des Jobs“, obwohl sich diese gravierend auf ihre Arbeit und ihr Leben auswirken können. Der intoxikierte oder psychiatrisch erkrankte männliche Patient steht hierbei ursächlich im Vordergrund. Leider stellt das in der Notfallmedizin tätige Personal durch Unerfahrenheit im Erkennen und in der Interpretation konfrontativer Situationen sowie einen Mangel an kommunikativen Fähigkeiten oft unwissentlich einen Teil der Eskalationsspirale dar.
Control of the polymyxin analog ratio by domain swapping in the nonribosomal peptide synthetase of Paenibacillus polymyxa
Authors: Yuan Ye , Xu Qiu-Man , Yu Si-Cen , Sun Hui-Zhong , Cheng Jing-Sheng , Yuan Ying-Jin 
Published: June 2020

Abstract
Polymyxins are used as the last-line therapy against multidrug-resistant bacteria. However, their further clinical development needs to solve problems related to the presence of heterogeneous analogs, but there is still no platform or methods that can regulate the biosynthesis of polymyxin analogs. In this study, we present an approach to swap domains in the polymyxin gene cluster to regulate the production of different analogs. Following adenylation domain swapping, the proportion of polymyxin B1 increased from 41.36 to 52.90%, while that of B1-1 decreased from 18.25 to 3.09%. The ratio of polymyxin B1 and B3 following starter condensation domain swapping changed from 41.36 and 16.99 to 55.03 and 6.39%, respectively. The two domain-swapping strains produced 62.96% of polymyxin B1, 6.70% of B3 and 3.32% of B1-1. This study also revealed the presence of overflow fluxes between acetoin, 2,3-butanediol and polymyxin. To our best knowledge, this is the first report of engineering the polymyxin synthetase gene cluster in situ to regulate the relative proportions of polymyxin analogs. This research paves a way for regulating lipopeptide analogs and will facilitate the development of novel lipopeptide derivatives.
Engineering Saccharomyces cerevisiae for production of the valuable monoterpene d-limonene during Chinese Baijiu fermentation
Authors: Hu Zhihui , Lin Liangcai , Li Hongxuan , Li Ping , Weng Yanru , Zhang Cuiying , Yu Aiqun , Xiao Dongguang 
Published: June 2020

Abstract
d-Limonene, a cyclic monoterpene, possesses citrus-like olfactory property and multi-physiological functions. In this study, the d-limonene synthase (t LS) from Citrus limon was codon-optimized and heterologously expressed in Saccharomyces cerevisiae. The metabolic flux of canonical pathway based on overexpressing endogenous geranyl diphosphate synthase gene (ERG20) and its variant ERG20F96W−N127W was strengthened for improvement d-limonene production in Chinese Baijiu. To further elevate production, we established an orthogonal pathway by introducing neryl diphosphate synthase 1 (t NDPS1) from Solanum lycopersicum. The results showed that expressing ERG20 and ERG20F96W−N127W could enhance d-limonene synthesis, while expressing heterologous NPP synthase gene significantly increase d-limonene formation. Furthermore, we constructed a t LS–t NDPS1 fusion protein, and the best strain yielded 9.8 mg/L d-limonene after optimizing the amino acid linker and fusion order, a 40% improvement over the free enzymes during Chinese Baijiu fermentation. Finally, under the optimized fermentation conditions, a maximum d-limonene content of 23.7 mg/L in strain AY12α-L9 was achieved, which was the highest reported production in Chinese Baijiu. In addition, we also investigated that the effect of d-limonene concentration on yeast growth and fermentation. This study provided a meaningful insight into the platform for other valuable monoterpenes biosynthesis in Chinese Baijiu fermentation.
The role of socioeconomic factors on discontinuation of insulin during pregnancy—methodological challenges from a Swedish register-based study
Authors: Zhao Liwei , Cesta Carolyn E. , Pazzagli Laura 
Published: June 2020

Abstract
Aim
Continuous use of antidiabetic medication, especially insulin, is crucial for diabetes management. In pregnancy, adherence to medication is generally low and may also be associated with lower socioeconomic status. However, little is known about discontinuation of insulin in pregnant women and the potential role of socioeconomic factors. Therefore, this study aims to measure the associations between socioeconomic factors and insulin discontinuation during pregnancy in women using insulin prior to the start of pregnancy in Sweden.

Subjects and methods
This cohort study identified pregnancies recorded in the Swedish Medical Birth Register from 2006 to 2016 in women receiving insulin prior to the start of pregnancy (N = 6029). Discontinuation of insulin was defined as not receiving a refill within 120 days from the previous refill. Associations between discontinuation and socioeconomic factors were investigated via logistic regression. Sensitivity analyses were performed using different definitions of discontinuation.

Results
Women discontinued insulin use in 34.2% of pregnancies. The odds ratios (ORs) of discontinuation of insulin were 1.17 [95% confidence interval (CI): 1.01–1.37] for women with lower household disposable incomes and 1.14 (95% CI: 1.01–1.29) for women with lower educational levels. When testing a change in the maximum refill gap from 91 days to 180 days, women with lower educational levels and those born in non-Nordic countries showed greater associations with discontinuation.

Conclusion
The definition of discontinuation affects the level of association of socioeconomic factors with discontinuation. Nevertheless, discontinuation of insulin is common in Sweden, especially in the lower socioeconomic groups. The socioeconomic inequality in insulin use behavior is worth noting.
Microenterprise Intervention to Reduce Sexual Risk Behaviors and Increase Employment and HIV Preventive Practices Among Economically-Vulnerable African-American Young Adults (EMERGE): A Feasibility Randomized Clinical Trial
Authors: Jennings Mayo-Wilson Larissa , Coleman Jessica , Timbo Fatmata , Ssewamala Fred M. , Linnemayr Sebastian , Yi Grace T. , Kang Bee-Ah , Johnson Matthew W. , Yenokyan Gayane , Dodge Brian , Glass Nancy E. 
Published: June 2020

AbstractEconomic vulnerability, such as homelessness and unemployment, contributes to HIV risk among U.S. racial minorities. Yet, few economic-strengthening interventions have been adapted for HIV prevention in this population. This study assessed the feasibility of conducting a randomized clinical trial of a 20-week microenterprise intervention for economically-vulnerable African-American young adults. Engaging MicroenterprisE for Resource Generation and Health Empowerment (EMERGE) aimed to reduce sexual risk behaviors and increase employment and uptake of HIV preventive behaviors. The experimental group received text messages on job openings plus educational sessions, mentoring, a start-up grant, and business and HIV prevention text messages. The comparison group received text messages on job openings only. Primary feasibility objectives assessed recruitment, randomization, participation, and retention. Secondary objectives examined employment, sexual risk behaviors, and HIV preventive behaviors. Outcome assessments used an in-person pre- and post-intervention interview and a weekly text message survey. Several progression criteria for a definitive trial were met. Thirty-eight participants were randomized to experimental (n = 19) or comparison group (n = 19) of which 95% were retained. The comparison intervention enhanced willingness to be randomized and reduced non-participation. Mean age of participants was 21.0 years; 35% were male; 81% were unemployed. Fifty-eight percent (58%) of experimental participants completed ≥ 70% of intervention activities, and 74% completed ≥ 50% of intervention activities. Participation in intervention activities and outcome assessments was highest in the first half (~ 10 weeks) of the study. Seventy-one percent (71%) of weekly text message surveys received a response through week 14, but responsiveness declined to 37% of participants responding to ≥ 70% of weekly text message surveys at the end of the study. The experimental group reported higher employment (from 32% at baseline to 83% at week 26) and lower unprotected sex (79% to 58%) over time compared to reported changes in employment (37% to 47%) and unprotected sex (63% to 53%) over time in the comparison group. Conducting this feasibility trial was a critical step in the process of designing and testing a behavioral intervention. Development of a fully-powered effectiveness trial should take into account lessons learned regarding intervention duration, screening, and measurement.Trial Registration ClinicalTrials.gov. NCT03766165. Registered 04 December 2018. https://clinicaltrials.gov/ct2/show/NCT03766165