bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-cell RNA-seq highlights heterogeneity in human primary Wharton’s jelly mesenchymal stem/stromal cells cultured in vitro
Authors: Sun Changbin , Wang Lei , Wang Hailun , Huang Tingrun , Yao Wenwen , Li Jing , Zhang Xi 
Published: April 2020

AbstractBackgroundMesenchymal stem/stromal cells (MSCs) are multipotent cells with a promising application potential in regenerative medicine and immunomodulation. However, MSCs cultured in vitro exhibit functional heterogeneity. The underlying molecular mechanisms that define MSC heterogeneity remain unclear.MethodsWe investigated the gene expression profile via single-cell RNA sequencing (scRNA-seq) of human primary Wharton’s jelly-derived MSCs (WJMSCs) cultured in vitro from three donors. We also isolated CD142+and CD142−WJMSCs based on scRNA-seq data and compared their proliferation capacity and “wound healing” potential in vitro. Meanwhile, we analyzed publicly available adipose-derived MSC (ADMSCs) scRNA-seq data and performed transcriptome comparison between WJMSCs and ADMSCs at the single-cell level.ResultsGO enrichment analysis of highly variable genes (HVGs) obtained from WJMSCs revealed that these genes are significantly enriched in extracellular region with binding function, involved in developmental process, signal transduction, cell proliferation, etc. Pathway analysis showed that these HVGs are associated with functional characteristics of classic MSCs, such as inflammation mediated by chemokine and cytokine signaling, integrin signaling, and angiogenesis. After regressing out the batch and cell cycle effects, these HVGs were used for dimension reduction and clustering analysis to identify candidate subpopulations. Differentially expressed gene analysis revealed the existence of several distinct subpopulations of MSCs that exhibit diverse functional characteristics related to proliferation, development, and inflammation response. In line with our data, sorted CD142+and CD142−WJMSCs showed distinct proliferation capacity as well as “wound healing” potential. Although WJMSCs and ADMSCs were derived from different tissues and were displaying different differentiation potencies, their HVGs were largely overlapped and had similar functional enrichment.ConclusionHVGs identified in MSCs are associated with classic MSC function. Regarding therapeutic potential, these genes are associated with functional characteristics, on which the MSC clinical application were theoretically based, such as development and inflammation response. Altogether, these HVGs hold the potential to be used as candidate markers for further potency association studies.
Existence and uniqueness of mild solutions to the chemotaxis-fluid system modeling coral fertilization
Authors: Hu Die , Chen Peng , Ma Deyi 
Published: April 2020

AbstractIn this paper, we consider the egg-sperm chemotaxis model of coral with the incompressible fluid equations in the whole space. The existence of global mild solutions in scaling invariant spaces is proved with sufficient small initial data. Here the main tool we use is the implicit function theorem. Furthermore, we obtain the asymptotic stability of solutions when the time goes to infinity. Since the initial data could be in the weak $L^{p}$Lp-spaces, we finally get the existence of self-similar solutions when the initial data are small homogeneous functions.
The asymptotic behavior of the stochastic coupled Kuramoto–Sivashinsky and Ginzburg–Landau equations
Authors: Lin Lin , Li Mei 
Published: April 2020

AbstractThe stochastic coupled Kuramoto–Sivashinsky and Ginzburg–Landau equations (KS-GL) perturbed by additive noises is investigated in this paper. By making careful analysis, we first consider the existence and uniqueness of the solution with initial-boundary condition, and then we establish a random attractor for the stochastic KS–GL equations in$X=L^{2} \times H^{-1}$X=L2×H−1.
A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community
Authors: Ye Xianfeng , Li Zhoukun , Luo Xue , Wang Wenhui , Li Yongkai , Li Rui , Zhang Bo , Qiao Yan , Zhou Jie , Fan Jiaqin , Wang Hui , Huang Yan , Cao Hui , Cui Zhongli , Zhang Ruifu 
Published: April 2020

Abstract
Background
Myxobacteria are micropredators in the soil ecosystem with the capacity to move and feed cooperatively. Some myxobacterial strains have been used to control soil-borne fungal phytopathogens. However, interactions among myxobacteria, plant pathogens, and the soil microbiome are largely unexplored. In this study, we aimed to investigate the behaviors of the myxobacterium Corallococcus sp. strain EGB in the soil and its effect on the soil microbiome after inoculation for controlling cucumber Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC).

Results
A greenhouse and a 2-year field experiment demonstrated that the solid-state fermented strain EGB significantly reduced the cucumber Fusarium wilt by 79.6% (greenhouse), 66.0% (2015, field), and 53.9% (2016, field). Strain EGB adapted to the soil environment well and decreased the abundance of soil-borne FOC efficiently. Spatiotemporal analysis of the soil microbial community showed that strain EGB migrated towards the roots and root exudates of the cucumber plants via chemotaxis. Cooccurrence network analysis of the soil microbiome indicated a decreased modularity and community number but an increased connection number per node after the application of strain EGB. Several predatory bacteria, such as Lysobacter, Microvirga, and Cupriavidus, appearing as hubs or indicators, showed intensive connections with other bacteria.

Conclusion
The predatory myxobacterium Corallococcus sp. strain EGB controlled cucumber Fusarium wilt by migrating to the plant root and regulating the soil microbial community. This strain has the potential to be developed as a novel biological control agent of soil-borne Fusarium wilt.
The relation of alexithymia and attachment with type 1 diabetes management in adolescents: a gender-specific analysis
Authors: Shayeghian Zeinab , Moeineslam Mina , Hajati Elnaz , Karimi Mehrdad , Amirshekari Golshan , Amiri Parisa 
Published: April 2020

AbstractBackgroundSeveral studies indicate the role of psychosocial factors in the management and control of chronic diseases in adolescents. In this regard, the roles of attachment and alexithymia in the management of type 1 diabetes in adolescents and related gender-specific patterns have rarely been the focus of empirical research. In this study we investigate the gender-specific relationship of alexithymia and attachment with self-care and blood glucose level in adolescents with type1 diabetes.MethodsThis is a cross-sectional study conducted on adolescents aged 12–18 years, with type 1 diabetes. Participants were recruited from diabetes clinics and the Iranian Diabetes Society. Data were collected using the Farsi versions of the Toronto Alexithymia Scale (FTAS-20), the Inventory of Parent and Peer Attachment (IPPA) and the Summary of Diabetes Self-Care Activities Scale (SDSCA). Blood glucose levels were measured by determining HbA1c which were abstracted from medical records. Data were analyzed using SPSS21 software.ResultsParticipants were 150 adolescents (57% female), mean age 14.97 ± 2.30. Alexithymia (β = 0.10,P = 0.01), difficulty identifying feelings (β = 0.15,P = 0.03) and communication with mothers (β = − 0.08,P = 0.03) predicted HbA1c in girls, whereas no significant relationships were observed for HbA1c with alexithymia and attachment in boys. Factors that predicted self-care in girls were alexithymia (β = − 0.04,P = 0.02), difficulty identifying feelings (β = − 0.06,P = 0.04); in boys however in addition to these two factors predicting self-care [alexithymia (β = − 0.07,P = 0.01) and difficulty identifying feelings (β = − 0.11,P = 0.01)], we also found difficulty describing feelings (β = − 0.16,P = 0.02), communication with mother (β = 0.04,P = 0.04), alienation to mother (β = − 0.06,P = 0.03), to father (β = − 0.06,P = 0.01) and to peers (β = − 0.09,P = 0.03).ConclusionsOur results suggest that, in a gender-specific pattern, alexithymia and attachment could affect self-care and blood glucose level in adolescents with type 1 diabetes; findings that can be used to facilitate more effective treatment strategies and interventions in this age group.
Phalaenopsis orchid miniaturization by overexpression of OsGA2ox6, a rice GA2-oxidase gene
Authors: Hsieh Kun-Ting , Liu Su-Hui , Wang I-Wen , Chen Liang-Jwu 
Published: April 2020

AbstractBackgroundPhalaenopsis orchids are one of the most common potted orchids sold worldwide. Most Phalaenopsis cultivars have long inflorescences that cause shipping problems and increase handling costs. Miniaturization of Phalaenopsis orchids not only reduces overall production costs but also can expand the appeal of the orchids to a different group of consumers who prefer to keep flowers on desks or tabletops. Although some miniature Phalaenopsis plants can be obtained via hybridization or mutation, they are unpredictable and limited in variety. We therefore used the transgenic approach of overexpressing gibberellin 2-oxidase 6 (OsGA2ox6), a rice GA deactivation gene, to investigate its functional effect in miniaturizing Phalaenopsis and to create a stable miniaturization platform to facilitate a supply for the potential demands of the miniature flower market.ResultsA commercial moth orchid,PhalaenopsisSogo Yukidian ‘SPM313’, was transformed with the plasmid vectorUbi:OsGA2ox6and successfully overexpressed theOsGA2ox6genein planta. The transgenic lines displayed darker-green, shorter, and wider leaves, thicker roots and much shorter flower spikes (10 cm vs 33 cm) than thenontransgenicline with a normal flower size and blooming ability and are therefore an ideal miniaturized form of Phalaenopsis orchids.ConclusionsWe demonstrated that the ectopic expression ofOsGA2ox6can miniaturizePhalaenopsisSogo Yukidian ‘SPM313’ while preserving its blooming ability, providing an alternative, useful method for miniaturizing Phalaenopsis species. This miniaturization by a transgenic approach can be further expanded by using GA2ox genes from different plant species or different gene variants, thereby expanding the technical platform for miniaturizing Phalaenopsis species to meet the potential demands of the miniature Phalaenopsis flower market.
Improved shift-invariant sparse coding for noise attenuation of magnetotelluric data
Authors: Li Guang , Liu Xiaoqiong , Tang Jingtian , Deng Juzhi , Hu Shuanggui , Zhou Cong , Chen Chaojian , Tang Wenwu 
Published: April 2020

AbstractMagnetotelluric (MT) method is widely used for revealing deep electrical structure. However, natural MT signals are susceptible to cultural noises. In particular, the existing data-processing methods usually fail to work when MT data are contaminated by persistent or coherent noises. To improve the quality of MT data collected with strong ambient noises, we propose a novel time-series editing method based on the improved shift-invariant sparse coding (ISISC), a data-driven machine learning algorithm. First, a redundant dictionary is learned autonomously from the raw MT data. Second, cultural noises are reconstructed using the learned dictionary and the orthogonal matching pursuit (OMP) algorithm. Finally, the de-noised MT data are obtained by subtracting the reconstructed cultural noises from the raw MT data. The synthetic data, field experimental data and measured data are tested to verify the effectiveness of the newly proposed method. The results show that our new scheme can effectively remove strong cultural noises and has better adaptability and efficiency than the predefined dictionary-based methods. The method can be used as an alternative when a remote reference station is not available.
Clinical efficacy and prognostic factors of CT-guided 125I brachytherapy for the palliative treatment of retroperitoneal metastatic lymph nodes
Authors: Yan Huzheng , Luo Ma , Wang Lifei , Qiu Zhenkang , Mo Zhiqiang , Xiang Zhanwang , Zhang Yanling , Chen Guanyu , Zhong Zhihui , Wang Xiuchen , Gao Fei , Zhang Fujun 
Published: April 2020

Abstract
Background
Due to the unique anatomical location of retroperitoneal metastatic lymph nodes, current treatment options are limited. This study was designed to explore the clinical efficacy and prognostic factors of CT-guided 125I brachytherapy for the treatment of retroperitoneal metastatic lymph nodes.

Methods
We retrospectively evaluated 92 patients received 125I brachytherapy for retroperitoneal metastatic lymph nodes. A layered Cox proportional hazards model was established to filter out the independent factors affecting local tumor progression-free survival (LTPFS).

Results
The median LTPFS was 8 months. Metastatic lymph node with uniform density (p-0.009), clear boundaries (p-0.011), regular morphology (P < 0.001), and < 3 organs at risk of metastasis (p-0.020) were associated with better LTPFS. Necrotic lymph nodes (p < 0.001), fusion (p-0.003), and invasion of vessels visible on images (p < 0.001) were associated with poor LTPFS. Puncture path through abdominal wall or paravertebral approach were also associated with better LTPFS than a hepatic approach (P < 0.05). A maximum diameter ≤ 3 cm (P-0.031) or 3–5 cm (P-0.018) were also associated with significantly better LTPFS than a maximum diameter ≥ 5 cm. The Cox proportional hazards model suggested that lymph nodes invaded the large vessels visible on images, maximum diameter and puncture path were independent risk factors for LTPFS.

Conclusion
CT-guided 125I brachytherapy is an optional palliative treatment modality for retroperitoneal metastatic lymph nodes, which can provide high local control without severe complications. Better preoperative planning, intraoperative implementation, better choice of puncture path, and selection of appropriate tumor size are important factors that can improve the clinical efficacy of 125I brachytherapy for retroperitoneal metastatic lymph nodes.
Thoracoscopic plication of the membranous portion of crescent-type tracheobronchomalacia in an elderly patient: a case report
Authors: Machino Ryusuke , Tagawa Tsutomu 
Published: April 2020

Abstract
Background
It is presumed that tracheobronchomalacia in adults is caused by airway pressure-induced injury due to chronic cough related to pulmonary emphysema or chronic bronchitis. Commonly, a posterolateral approach using stabilizing materials is the surgical technique of choice for treating tracheobronchomalacia. We report a case in which thoracoscopic plication of the membranous portion was performed instead of airway stent placement for tracheobronchomalacia in an elderly individual.

Case presentation
An 87-year-old man who had been treated for bronchial asthma, pulmonary emphysema, and tracheobronchomalacia was admitted to our hospital with acute exacerbation of dyspnea. The patient underwent tracheal intubation, which was followed by tracheostomy 16 days later. Insertion of the tip of the adjustable-length tracheostomy tube to the end of the stenotic lesion enabled him to breathe spontaneously. However, conservative management failed due to recurrent pneumonia caused by the tracheobronchomalacia. Crescent-type tracheobronchomalacia (Johnson’s classification grade III) was diagnosed, and the main narrowed area of the trachea was assumed to be approximately 3–10 cm from the tracheal bifurcation. A thoracoscopic approach was selected because a posterolateral approach was considered too invasive considering the patient’s age and general condition. We placed eight stitches on the tracheal membranous portion and four stitches on the membranous portion of the right main bronchus, using the horizontal mattress suture technique. The use of foreign materials was avoided because meropenem-resistant Pseudomonas aeruginosa was cultured in a tracheal specimen. Immediately after the operation, the expiratory airway stenosis improved, and subsequently, spontaneous ventilation was possible using a normal type of tracheostomy tube instead of an adjustable-length tracheostomy tube.

Conclusions
Tracheobronchomalacia is not a rare condition in patients with chronic obstructive pulmonary disease. The thoracoscopic approach is less invasive than the posterolateral approach and is suitable in cases that are otherwise refractory to medical treatment. We believe that thoracoscopy may be a useful treatment option in cases where conservative treatment is not appropriate.
Role of PET/CT in patients with unexplained rising alpha fetoprotein post HCC interventional management
Authors: Ragheb Samar Ramzy , Abelsamad Amr Mahmoud Ahmed , Abelkawy Moustafa Mahmoud , Nasr Osama Abu Elnaga Khalaf 
Published: April 2020

Abstract
Background
Positron emission tomography–computed tomography (PET/CT) is considered a powerful modality in the follow-up of hepatocellular carcinoma (HCC) patients. In this study, PET/CT was done in an evaluation of patients with unexplained rising alpha fetoprotein (AFP) post hepatocellular carcinoma (HCC) interventional management in 40 patients (16 females and 24 males); their age ranged from 25 to 82 years, had undergone interventional management for HCC and underwent PET/CT follow-up within an 8-month duration from their intervention. Whole-body PET/CT was performed after injection of (18)-FDG, and the results were read in a masked manner by two specialists, and diagnostic performance was assessed from the results of consensus masked reading. All the results were evaluated with the Barcelona criteria and biopsy correlation.

Results
During the follow-up PET/CT, 24 patients had complete response and 8 patients showed focal residual while the rest 8 patients showed newly developed lesions.

Conclusion
PET/CT is an excellent method for the evaluation of HCC patients with equivocal results after interventional management.
A global needs assessment in times of a global crisis: world psychiatry response to the COVID-19 pandemic
Authors: Kaufman Kenneth R. , Petkova Eva , Bhui Kamaldeep S. , Schulze Thomas G. 
Published: April 2020

Summary
The COVID-19 pandemic has stunned the global community with marked social and psychological ramifications. There are key challenges for psychiatry that require urgent attention to ensure mental health well-being for all – COVID-19-positive patients, healthcare professionals, first responders, people with psychiatric disorders and the general population. This editorial outlines some of these challenges and research questions, and serves as a preliminary framework of what needs to be addressed. Mental healthcare should be an integral component of healthcare policy and practice towards COVID-19. Collaborative efforts from psychiatric organisations and their members are required to maximise appropriate clinical and educational interventions while minimising stigma.
Actin waves transport RanGTP to the neurite tip to regulate non-centrosomal microtubules in neurons
Authors: Huang Yung-An , Hsu Chih-Hsuan , Chiu Ho-Chieh , Hsi Pei-Yu , Ho Chris T. , Lo Wei-Lun , Hwang Eric 
Published: April 2020

ABSTRACT
Microtubules (MTs) are the most abundant cytoskeleton in neurons, and control multiple facets of their development. While the MT-organizing center (MTOC) in mitotic cells is typically located at the centrosome, the MTOC in neurons switches to non-centrosomal sites. A handful of cellular components have been shown to promote non-centrosomal MT (ncMT) formation in neurons, yet the regulation mechanism remains unknown. Here, we demonstrate that the small GTPase Ran is a key regulator of ncMTs in neurons. Using an optogenetic tool that enables light-induced local production of RanGTP, we demonstrate that RanGTP promotes ncMT plus-end growth along the neurite. Additionally, we discovered that actin waves drive the anterograde transport of RanGTP. Pharmacological disruption of actin waves abolishes the enrichment of RanGTP and reduces growing ncMT plus-ends at the neurite tip. These observations identify a novel regulation mechanism for ncMTs and pinpoint an indirect connection between the actin and MT cytoskeletons in neurons.
TRATAMENTO DO COVID‑19 COM MEDICAMENTOS EXPERIMENTAIS EM TESTES CLÍNICOS: DESAFIOS E PERSPECTIVAS
Authors: Oliveira Anselmo Gomes de , Silveira Dâmaris 
Published: April 2020

O primeiro número de 2020 de Infarma – Ciências Farmacêuticas está sendo publicado em meio a uma pandemia que avassala toda a população mundial, sem distinção de raça, cor, credo ou situação social e econômica. E, em um cenário no qual o número de mortos se multiplica exponencialmente, comprometendo qualquer sistema de saúde vigente, a busca, às vezes sem critério, por fármacos que possam combater e eliminar o agente causal ocupa uma posição de destaque. E notícias sobre “remédios milagrosos” são espalhadas em questão de segundos, levando a uma corrida frenética em busca da possibilidade de cura ou prevenção dacontaminação.
PROCESSO DE PADRONIZACAO DE MEDICAMENTOS ORAIS PARA ADMINISTRACAO VIA SONDA ENTERAL PARA HOSPITAL UNIVERSITÁRIO MATERNO‑INFANTIL
Authors: Da Silva Isadora Dávila , Silva Nunes Michelle , Mariz Batista Almária 
Published: April 2020

A adaptação de formas farmacêuticas orais para uso via sonda enteral é necessária diante da escassez de formulações, especialmente para pacientes pediátricos, pacientes com transtornos de deglutição e pacientes críticos internados em UTI. É necessária a avaliação do farmacêutico quando há necessidade de adaptar forma farmacêutica, para constatar se realmente é permitido fazê‑lo, se não há alternativa terapêutica disponível e, em caso de viabilidade de adaptação, realizar o procedimento. O estudo teve como objetivo a avaliação do processo de padronização de medicamentos administrados via oral que podem ser adaptados para administração via sonda enteral. Para isso, foi utilizada como base a lista de padronização de medicamentos da Maternidade Escola Januário Cicco e a consulta foi realizada nas fontes Micromedex, Dynamed, Handbook of Drug Administration via Enteral Feeding Tubes e Drugs.com. Das 68 formas farmacêuticas orais (63 princípios ativos), 76,47% podiam ser adaptadas e administradas por sonda enteral e para 13,23% não foram encontrados dados na literatura acerca da viabilidade da adaptação destas formas farmacêuticas. Além disso, 23,80% dos medicamentos possuíam vias alternativas de administração. Foram encontradas 46 interações medicamento‑alimento, sendo 15,22% da categoria Importante, 63,04% Moderada e 21,74% Menor/Secundária. As informações sobre adaptações de formas farmacêuticas para administração via sonda enteral ainda são escassas na literatura, sendo, portanto, imprescindível a compilação das informações disponíveis acerca do tema, uma vez que a não observância destas pode comprometer a segurança do paciente.