bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Chloroplast Genome Analysis of Impatiens Species (Balsaminaceae) in the Karst Area of China: Insights Into Genome Evolution and Phylogenomic Implications.
Authors: Luo Chao , Huang WuLue , Sun Huayu , Yer Huseyin , Li Xinyi , Li Yang , Yan Bo , Wang Qiong , Wen Yonghui , Huang Meijuan , Huang Haiquan 
Published: January 2021

Abstract
Background: Impatiens, a controversial and complex genus, which belongs to the family Balsaminaceae with approximately 1000 species. The genus is well known for economical, medicinal, ornamental, and horticultural values. However, Due to the morphological features and insufficient genomic resources, their analyses of germplasm identification and molecular phylogeny are very limited. Results: We have sequenced the chloroplast genomes of six different species (I. chlorosepala, I. fanjingshanica, I. guizhouensis, I. linearisepala, I. loulanensis, and I. stenosepala) in the karst area of China and compared them with previously published species and the monospecific sister Hydrocera triflora belonging to Balsaminaceae family. We comtrasted the genome features, repeat sequences, sequence divergence, and constructed the phylogenetic relationships. The 12 complete chloroplast genomes of the Balsaminaceae species ranged in size from 151,538 bp (I. fanjingshanica) to 154,189bp (H. triflora ) encoded 114 total distinct genes except for I. glandulifera and H. triflora, including 81 protein-coding, 29 transfer RNA genes(tRNA), and 4 ribosomal RNA genes (rRNA). Moreover, the characteristics of the long repeats sequences and simple sequence repeats (SSRs) were found. Divergent hotspots regions psbK-psbI,trnT-GGU-psbD, rpl36-rps8, rpoB-trnC-GCA, trnK-UUU-rps16, trnQ-UUG, trnP-UGG-psaJ, trnT-UGU-trnL-UAA, and ycf4-cemA were identified in the 12 Balsaminaceae chloroplast genomes, which could be suitable for species identification and phylogenetic studies. Additionally, phylogenetic relationships based on Maximum likelihood (ML) and Bayesian inference (BI) among whole chloroplast genomes showed that H. triflora was the basal group in Balsaminaceae and I. guizhouensis was the basal group in impatiens species. Besides this, cultivated species(I. balsamina, I. hawkeri, and I. walleriana) were clustered together. Conclusion: Our study provides detailed information about nucleotide diversity hotspots and characterized types of repeats, which can be used for developing molecular markers applicable in Balsaminaceae species. And also we reconstructed and analyzed the relationships of some impatiens species and discovered their status based on the complete chloroplast genomes. The current study might provide valuable significant genomic information for the systematics and evolution in the Balsaminaceae.
Prevalence of Hereditary Cancer Susceptibility Syndromes (HCSS) in Acute Lymphoblastic Leukemia (ALL): A Cross-Sectional Study in a Highly Consanguineous Population.
Authors: Aslam Sara , NA Shabana , Ahmed Mehboob 
Published: January 2021

Abstract
Background: Hereditary cancer susceptibility syndrome (HCSS) has been reported to impact cancer predisposition at an early age, therefore, identification of HCSS has found to be crucial for surveillance, managing therapeutic interventions and referring the patients and their families for genetic counselling. The aims of this study are to assess the prevalence of HCSS as hereditary leukemia and hematologic malignancy syndrome by using ACMG guidelines and to assess parental consanguinity as the criterion for referring patients for the genetic counselling. Methods: A total of 300 acute lymphoblastic leukemia subjects were recruited from the Children’s Hospital, Lahore, Pakistan during the period of December 2018 to September 2019. Structured self-reporting questionnaire based on family and medical history of the disease was utilized for the data collection.Results: In our cohort, 60.40% of ALL patients were identified to have HCSS and among them 40.65% patients solely fulfil the criteria due to the presence of parental consanguinity. Parental consanguinity was shown to have protective impact on the onset at early age of disease [OD=0.44 (0.25-0.77), p-value= 0.00] while family history of cancer increase the risk of cardiotoxicity [OD= 2.46 (1.15-5.24), p-value=0.02]. Parental consanguinity in the population shows no significant impact on the family history of cancer and the number of relatives with cancer. Conclusions: The higher prevalence of HCSS in Pakistani population is attributed to the presence of parental consanguinity in more than 50% of the patients when assessed through ACMG guidelines. Our study suggests revisiting ACMG guidelines for the criterion of parental consanguinity in the highly consanguineous population and formulating the score based criteria for the identification of inherited ALL for genetic counselling.
Sexual Dysfunction in Iranian Older Women and its Predictors: A Cross- Sectional Study
Authors: Naeeni Masoumeh Rajabi- , Dehdari Tahereh , Jamshidimanesh Mansoureh , Janani Leila 
Published: January 2021

Abstract
Background: The population of older women is rapidly increasing in every country in the world, including Iran. Given the effect of Sexual Function (SF) and sexual health on older women’s health, the present study was conducted to determine Sexual Dysfunction (SD) and the factors predicting it in Iranian older women in Karaj. Methods: The present cross-sectional study was conducted on 550 eligible older women selected by stratified cluster sampling from those visiting health centers in Karaj between October 2019 and February 2020. Data were collected using self-report questionnaires including demographic questionnaire, the hospital anxiety and depression scale (HADS), and the Female Sexual Function Index (FSFI). To take account of the effect of sampling design on the analyses, survey command in Stata-14 was used. Results: A total of 94.5% of the participating women had SD, 74.4% reported desire disorder, 79.8% arousal disorder, 57.6% lubrication disorder, 65.3% orgasm disorder, and 72.2% sexual dissatisfaction, and 52.4% had dyspareunia. The participating women’s SF had significant relationships with physical activity, hysterectomy, spouse’s SD, and the intimate and emotional closeness with the spouse (P<0.05). SF had significant inverse relationships with age, duration of marriage and menopause, anxiety, and depression (P<0.001). Nonetheless, SF had no significant relationship with occupation, education, smoking, and type and frequency of childbirth. The results of the linear regression test showed that the duration of menopause (B=-0.04, P=0.023), spouse’s SD (B=-0.95, P<0.001), intimacy (B=13.89, P<0.001), emotional closeness (B=6.49, P<0.001), anxiety (B=-0.08, P=0.033), and depression (B=-0.24, P<0.001) predicted the participating women’s SF significantly. Conclusion: The majority of the participating women had SD. Attention mental health, spousal relationship, and spouse’s sexual health may have significant positive effects on the sexual health of Iranian older women.
Bacteroides Uniformis CECT 7771 Alleviates Inflammation Within the Gut-adipose Tissue Axis, Involving TLR5 Signaling, in Diet-Induced Obese Mice
Authors: Fabersani Emanuel , Portune Kevin , Campillo Isabel , López-Almela Inmaculada , Paz Sergio Montserrat-de la , Romaní-Pérez Marina , Benítez-Páez Alfonso , Sanz Yolanda 
Published: January 2021

Abstract
This study investigated the immune mechanisms whereby administration of Bacteroides uniformis CECT 7771 reduces metabolic dysfunction in obesity. C57BL/6 adult male mice were fed a standard diet or a Western diet high in fat and fructose, supplemented or not with B. uniformis CECT 7771 for 14 weeks. B. uniformis CECT 7771 reduced body weight gain, plasma cholesterol, triglyceride, glucose, and leptin levels; and improved oral glucose tolerance in obese mice. Moreover, B. uniformis CECT 7771 modulated the gut microbiota and immune alterations associated with obesity, increasing Tregs and reducing B cells, total macrophages and the M1/M2 ratio in both the gut and epididymal adipose tissue (EAT) of obese mice. B. uniformis CECT 7771 also increased the concentration of the anti-inflammatory cytokine IL-10 in the gut, EAT and peripheral blood, and protective cytokines TSLP and IL-33, involved in Treg induction and type 2 innate lymphoid cells activation, in the EAT. It also restored the obesity–reduced TLR5 expression in the ileum and EAT. The findings indicate that the administration of a human intestinal bacterium with immunoregulatory properties on the intestinal mucosa helps reverse the immuno-metabolic dysfunction caused by a Western diet acting over the gut-adipose tissue axis.
Fecal Microbiota Dynamics During Disease Activity and Remission in Newly Diagnosed and Established Ulcerative Colitis
Authors: Öhman Lena , Lasson Anders , Strömbeck Anna , Isaksson Stefan , Hesselmar Marcus , Simrén Magnus , Strid Hans , Magnusson Maria 
Published: January 2021

Abstract
Patients with ulcerative colitis (UC) have an altered gut microbiota composition, but the microbial relationship to disease activity needs to be further elucidated. Therefore, temporal dynamics of the fecal microbial community during remission and flare was determined. Fecal samples were collected at 2–6 time-points from UC patients during established disease (cohort EST) and at diagnosis (cohort NEW). Sampling range for cohort EST was 3–10 months and for cohort NEW 36 months. Relapses were monitored for an additional three years for cohort EST. Microbial composition was assessed by Genetic Analysis GA-map™ Dysbiosis test, targeting ≥ 300 bacteria. Eighteen patients in cohort EST (8 with maintained remission and 10 experiencing a flare), provided 71 fecal samples. In cohort NEW, 13 patients provided 49 fecal samples. The microbial composition showed no clustering related to disease activity in any cohort. Microbial dissimilarity was higher between than within patients for both cohorts, irrespective of presence of a flare. Microbial stability within patients was constant over time with no major shift in overall composition nor modification in the abundance of any specific species. Microbial composition was not affected by intensified medical treatment or linked to future disease course. Thus in UC, the gut microbiota is highly stable irrespective of disease stage, disease activity or treatment escalation. This suggests that prolonged dietary interventions or repeated fecal transplantations are needed to be able to induce permanent alterations of the gut microbiota.
Sustainability, Productivity, Profitability and Soil Health With Conservation Agriculture-Based Sustainable Intensification of Oilseed Brassica Production System
Authors: Jat R.S. , Choudhary R.L. , Singh H.V. , Meena M.K. , Singh V.V. , Rai P.K. 
Published: January 2021

Abstract
Conservation Agriculture (CA) practices getting space world-wide to answer many emerging challenges like; declining factor productivity, deteriorating soil health, water scarcity, climate change, and farm profitability and sustainability. Oilseed brassica (Indian mustard, Brassica juncea L.), a winter oilseed grown under rainfed agro-ecosystem vulnerable to low yields, high production cost, degrading soil and water quality, and climatic vagaries. The present study was undertaken on CA-based sustainable intensification of Indian mustard for enhancing inputs efficiencies, farm profitability and sustainability. Permanent beds with residue retention (PB+R) improved mustard equivalent yield (11.4%) and system grain yield (10.6%) compared to conventional tillage without residue (CT-R). Maize-mustard (Mz-M) increased system grain yield (142.9%) as well as mustard equivalent yield (60.7%) compared to fallow-mustard (F-M). Mz-M system under PB+R increased sustainable yield index (376.5%), production efficiency (177.2%), economic efficiency (94%) and irrigation water productivity (66%) compared to F-M under CT-R. PB+R increased soil organic carbon (SOC) stock at 0-15 cm (17.7%) and 15-30 cm (29.5%) soil depth compared to CT-R. Intensification of F-M system with green gram–mustard (GG-M) improved SOC at 0-15 cm (27.4%) and 15-30 cm (20.5%) compared to F-M. CA-based cluster bean-mustard/GG-M system increased N productivity, whereas, P and K productivity improved with Mz-M system compared to F-M under CT-R. Thus, CA-based Mz-M system should be out-scaled in the traditional rainfed fallow-mustard system to improve the farm production and income on holistic basis to make the country self-sufficient in edible oils.
What Facilitates and Hinders the Implementation of Nurse-Led Interventions in Long-Term Dementia Care? A Qualitative Interview Study With Swiss Nursing Experts and Managers
Authors: Hirt Julian , Karrer Melanie , Adlbrecht Laura , Saxer Susi , Zeller Adelheid 
Published: January 2021

Abstract
Background: To support the implementation of nurse-led interventions in long-term dementia care, in-depth knowledge of specific supporting factors and barriers is required. Conditions and structures of caring for people with dementia differ widely, depending on the country and the care context. Our study aimed to describe the experiences and opinions of nursing experts and managers with regard to facilitators and barriers to the implementation of nurse-led interventions in Swiss long-term dementia care.Methods: We conducted a qualitative descriptive study using individual interviews based on qualitative vignettes as a useful stimulus to generate narrations allowing to study peoples’ perceptions and beliefs. The study took place in nursing homes in the German-speaking part of Switzerland and in the Principality of Liechtenstein using purposive sampling. We intended to conduct the interviews face-to-face in a quiet room according to the participant’s choice. However, due to the lockdown of nursing homes during the COVID-19 pandemic in spring 2020, we performed interviews face-to-face and by video. We analysed data thematically following Braun and Clarke to achieve a detailed, nuanced description. To verify our interpretation and to ensure congruence with participants’ perspectives, we conducted member checks. The Standards for Reporting Qualitative Research (SRQR) served to structure our manuscript.Results: Six dyads of nursing home managers and nursing experts from six nursing homes took part in our study (n=12). Our thematic analysis yielded seven themes reflecting facilitators and barriers to implementing nurse-led interventions in Swiss long-term dementia care: «A common attitude and cohesion within the organization», «Commitment on several levels», «A needs-oriented implementation», «The effect and the public perception of the intervention», «A structured and guided implementation process», «Supporting knowledge and competencies», as well as «Resources for implementing the intervention».Conclusions: Successful implementation of nurse-led interventions in dementia care is highly challenging due to the complex nature of interventions. Therefore, it is important to know the facilitators and barriers to optimally design implementation strategies. Our results might support researchers and other key persons involved in implementing nurse-led interventions in Swiss long-term dementia care.
Torsion of Epiploic Appendix Following Chiropractic Movements: A Case Report
Authors: Etezadpour Mohammad , Sharifi Seyed Hassan Seyed , Behnamfar Morteza , Hosseinzadeh Rezvan 
Published: January 2021

Abstract
BackgroundAn epileptic appendix torsion is a rare event that can lead to acute abdominal pain and occurs at any age. Chiropractic is a type of complementary medicine that is performed explicitly by spinal manipulations. Specialists use spinal manipulation to treat many musculoskeletal disorders, usually mechanical back and neck pain, headaches, and spinal stiffness. Case presentationA 49-year-old man with a history of progressive diffuse abdominal pain predominately in the left lower quadrant started three days ago was referred to Ghaem Hospital, Mashhad, Iran. All laboratory tests were normal, and no abnormal findings were observed on ultrasound and X-rays, but the CT scan revealed a definite diagnosis of appendicular torsion. Gastrointestinal rest, antibiotics, analgesics, and fluid therapy were started for the patient. After 72 hours, the patient's general condition improved, and he was discharged from the hospital. In this report, we presented a case who developed acute abdominal pain after spinal manipulation. Chiropractic is not a completely safe treatment and can cause irreversible damage, so we should know this method's dangers and have enough experience to provide the best and safest medical services for patients.
Impact of Working Together for Adults with Autism Spectrum Disorder: A Multifamily Group Intervention
Authors: DaWalt Leann , Hickey Emily , Hudock Rebekah , Esler Amy , Mailick Marsha 
Published: January 2021

Abstract
Background. Adults with autism spectrum disorder (ASD) have lower participation in post-secondary education, higher rates of unemployment/underemployment, and continued difficulties with challenging behavior and mental health problems compared to their peers. Multi-family psychoeducation emphasizes education and problem-solving with the goal of improving outcomes for the individual with the disability.Methods. Using a randomized waitlist control design, the present study evaluated a multi-family group psychoeducation intervention, Working Together, for adults on the autism spectrum without intellectual disability (n=40). Five waves of data were collected at three-month intervals. In this design, families in the intervention condition participated in intervention during the 6 months between baseline and Time 3 data collection; the waitlist control condition received the intervention immediately after the Time 3 data collection. We compared these two conditions, intervention group vs waitlist control group, on key outcomes for the adults with ASD: engagement in work, engagement in positive activities, and behavior problems.Results. Results indicated that the Working Together intervention was associated with increases in engagement as well as decreases in behavior problems, particularly internalizing problems. We also found maintenance of the treatment effect through 6 months post-treatment for the intervention group and replication of the treatment effect within the control group after they received the intervention.Conclusion. Working Together is a promising multi-family group psychoeducation intervention designed to improve functioning during adulthood. These findings highlight the need for more intervention services research during adulthood and specifically the need for family-centered supports.
In Vitro Antioxidant, Membrane Stabilizing and Antimicrobial Properties of Murayya paniculata Wild Plant of Amarkantak (M.P)
Authors: Sonter Shruti , Mishra Shringika , Dwivedi Manish , Singh Prashant 
Published: January 2021

Abstract
The excessive usage of antibiotics in humans and veterinary medicine has lead to the emergence of antibiotic resistance and requirement of novel antibiotics. There has been increased interest towards plants as source of drugs because of their pharmacological potency and long traditional usage. The aim of the current study was to evaluate bioactive components, antioxidant and anti-inflammatory activity from the leaf extracts of Murayya paniculata, a traditionally used Indian medicinal plant. Evaluations were made for phytochemicals, antioxidant, membrane stabilizing and antimicrobial activities. The methanol extract displayed highest flavonoid and phenolic content while the acetone extract demonstrated considerable ABTS inhibitory activity (IC50value:555.18 ± 1.68 µg/mL) and hexane extract exhibited highest H2O2 radical scavenging activity (IC50value: 509.84 ± 3.03 µg/mL). The aqueous extract displayed 19.4 ± 0.66% RBC hemolysis and 80.5 ± 0.66% protection caused by hypotonic solution at high concentration of extract. The fractions of hexane extract revealed a higher zone of inhibition than crude extract. The major components found in the fractions were cyclohexane (40.11%) and 3-(6-Methoxy-3-methyl-2-benzofuranyl) Cyclohexanone (13.68%) analyzed by GC-MS technique. The current results validate the traditional use of the plant and warrant its potential in drug development programs in further investigations.
Overexpression of PVR&nbsp;and PD-L1&nbsp;and Its Association With Prognosis in Surgically Resected Squamous Cell Lung Carcinoma&nbsp;
Authors: Lee Jii Bum , Hong Min Hee , Park Seong Yong , Chae Sehyun , Hwang Daehee , Ha Sang-Jun , Shim Hyo Sup , Kim Hye Ryun 
Published: January 2021

Abstract
Background: Targeting T-Cell Immunoreceptor with Ig and ITIM domain-poliovirus receptor (PVR) pathway is a potential therapeutic strategy in lung cancer. We analyzed the expression of PVR and programmed death ligand-1 (PD-L1) in surgically resected squamous cell lung carcinoma (SQCC) and determined its prognostic significance.Methods: We collected archival surgical specimens of 259 patients with SQCC at Yonsei Cancer Center (1998–2020). Analysis of variance was used to analyze the correlations between PVR and PD-L1 expression and patient characteristics. Kaplan–Meier curves were used to estimate recurrence-free survival (RFS) and overall survival (OS). Results: Most patients were male (93%); the majority were diagnosed with stage 1 (47%), followed by stage 2 (29%) and stage 3 (21%). Overexpression of PVR resulted in a significantly shorter median RFS and OS (P = 0.01). PD-L1 expression was not significant in terms of prognosis. Patients were subdivided into four groups based on low and high PVR and PD-L1 expression. Those expressing high levels of PVR and PD-L1 had the shortest RFS (P = 0.03).Conclusions: PVR overexpression is associated with a poor prognosis in surgically resected SQCC. Inhibition of PVR as well as PD-L1 may help overcome the lack of response to immune checkpoint monotherapy.
Helicobacter Pylori Infection Increases the Incidence of Erosive Oral Lichen Planus and Alters the Oral Microbiome Composition
Authors: Zhang Yangheng , Li Shutong , Li Jingyuan , Li Ya , Li Huanjie , Li Wenjuan , Jia Jihui , Ge Shaohua , Sun Yundong 
Published: January 2021

Abstract
Background Oral lichen planus (OLP), a common clinical oral disease, is associated with an increased risk for malignant transformation. The mechanism underlying the pathogenesis of OLP is unknown. Oral dysbacteriosis is reported to be one of the etiological factors for OLP. Although H. pylori infection is associated with various oral diseases, the correlation between H. pylori infection and OLP is unclear. This study aimed to investigate the effect of H. pylori infection on OLP pathogenesis and oral microbiome composition in the Chinese population, which has a high incidence of H. pylori infection.Result In this study, the saliva samples of 30 patients with OLP (OLP group) and 21 negative controls (NC group) were collected. H. pylori infection was detected using the carbon-14 urea breath test. The saliva samples were divided into the following four groups based on the H. pylori infection status: H. pylori-positive OLP (OLP+), H. pylori-positive NC (NC+), H. pylori-negative OLP (OLP−), and H. pylori-negative NC (NC−) groups. The oral microbiome composition was significantly different between the OLP and NC groups and between the OLP− and OLP+ groups. Compared with those in the OLP− group, the incidence of erosive OLP and the salivary levels of cytokines were higher in the OLP+ group. In contrast, the oral microbiome composition and cytokine levels were not significantly different between the NC− and NC+ groups.Conclusions This is the first report demonstrated that H. pylori infection is significantly correlated with the pathogenesis of erosive OLP. The alleviation of H. pylori infection may decrease the incidence of erosive OLP.