bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nicardipine Versus Nitroglycerin for The Treatment of Hypertensive Acute Heart Failure Syndrome: A Single-Center Observational Study
Authors: Koroki Takatoshi , Abe Tomohiro , Ochiai Hidenobu 
Published: December 2020

Abstract
Background: Nitroglycerin is considered a first-line agent for hypertensive acute heart failure syndromes (AHFS). In this study, we compared the effectiveness of nicardipine and nitroglycerin in patients with hypertensive AHFS. Methods: We conducted a single-center, retrospective, observational study at the intensive care unit of a Japanese hospital. Patients diagnosed with AHFS and systolic blood pressure exceeding 140 mmHg on arrival between April 2013 and August 2019 were included. The outcomes were the time to optimal blood pressure control, duration of the continuous infusion of anti-hypertensive agents, duration of positive pressure ventilation, need for additional anti-hypertensive agents, length of hospital stay, and body weight changes. Outcomes were compared between the nicardipine and nitroglycerin groups. We also compared these outcomes between the groups after excluding patients who received renal replacement therapy. Results: Forty-five patients were enrolled (25 and 20 were treated with nitroglycerin and nicardipine, respectively). The nicardipine group had a shorter time to optimal blood pressure control (1.0 [interquartile range, 1.0–3.0] h vs. 0.5 [0.5–1.0] h), shorter duration of the continuous infusion of anti-hypertensive agents (65.5 [32.5–127.5] h vs. 31.0 [18.5–61.0] h), less frequent need for additional anti-hypertensive agents (0 patients vs. 10 patients [40.0%]), and shorter length of hospital stay (19.0 [10.0–33.0] days vs. 8.0 [5.0–12.3] days) than the nitroglycerin group. The duration of positive pressure ventilation and body weight changes were similar between the groups. These outcomes were similar after excluding patients who received renal replacement therapy. Conclusions: Nicardipine may be more effective than nitroglycerin for treating hypertensive AHFS.
Disentangling archaeal and bacterial biogeography and co-occurrence networks in subtropical mangrove sediments under Spartina alterniflora invasion
Authors: Chen Weidong , Wen Donghui 
Published: December 2020

Abstract
Background Mangrove ecosystems are vulnerable due to the exotic Spartina alterniflora invasion in China. Large knowledge gaps remain with regard to archaeal and bacterial communities assembly processes and microbial interactions under S. alterniflora invasion. Here, using 16S rRNA gene amplicon sequencing, we investigated the assembly processes and co-occurrence relationships of the archaeal and bacterial communities under S. alterniflora invasion along the coastlines of Fujian province, southeast China.Results We found that the overall archaeal and bacterial communities were driven predominantly by stochastic processes, and the relative role of stochasticity was stronger for bacteria than archaea. Co-occurrence network analysis showed that the network structure of bacteria was more complex than that of the archaea. Putative keystone taxa often had low relative abundances (conditionally rare taxa), suggesting conditionally rare taxa or low abundances taxa may significantly contribute to network stability. Moreover, we found that S. alterniflora invasion changed the microbial communities assembly and co-occurrence patterns, indicating that S. alterniflora affected the composition and stability of the microbial community.Conclusions This study provides the first comparison in the biogeography and co-occurrence patterns of both archaea and bacteria in mangrove ecosystem. And this is the first exploration about the effect of S. alterniflora invasion on archaeal and bacterial ecological processes and co-occurrence patterns. Our study considers that the control of S. alterniflora invasion is important for mangrove ecosystem function and service.
Six-month follow-up of functional status in discharged patients with coronavirus disease 2019
Authors: Du Houwei , Fang Shuang-fang , Wu Sang-ru , Chen Xiao-ling , Chen Jun-nian , Zhang Yi-xian , Huang Hua-yao , Lei Han-han , Chen Rong-hua , Pan Xiao-bin , Li Xiao-qing , Xia Pin-cang , Zheng Zhen-yang , Lin Hai-long , Chen Li-min , Liu Nan 
Published: December 2020

Abstract
Background The long-term functional outcome of discharged patients with coronavirus disease 2019 (COVID-19) remains unresolved. We aimed to describe a six-month follow-up of functional status of COVID-19 survivors.Methods We reviewed the data of COVID-19 patients who had been consecutively admitted to the Tumor Center of Union Hospital (Wuhan, China) between 15 February and 14 March 2020. We quantified a six-month functional outcome reflecting symptoms and disability in COVID-19 survivors using a post-COVID-19 functional status scale ranging from 0 to 5 (PCFS). We examined the risk factors for the incomplete functional status defined as a PCFS > 0 at a six-month follow-up after discharge.Results We included a total of 95 COVID-19 survivors with a median age of 62 (IQR 53-69) who had a complete functional status (PCFS grade 0) at baseline in this retrospective observational study. At six-month follow-up, 67 (70.5%) patients had a complete functional outcome (grade 0), 9 (9.5%) had a negligible limited function (grade 1), 12 (12.6%) had a mild limited function (grade 2), 7 (7.4%) had moderate limited function (grade 3). Univariable logistic regression analysis showed a significant association between the onset symptoms of muscle or joint pain and an increased risk of incomplete function (unadjusted OR 4.06, 95%CI 1.33 - 12.37). This association remained after adjustment for age and admission delay (adjusted OR 3.39, 95%CI 1.06 - 10.81, p = 0.039).Conclusions A small proportion of discharged COVID-19 patients may have an incomplete functional outcome at a six-month follow-up; intervention strategies are required.
Recovery of Olfactory and Gustatory Dysfunctions in COVID-19 Patients in Iran: A Prospective Cohort Study
Authors: Balajelini Mohammad Hosein Taziki , Vakili Mohammad Ali , Rajabi Abdolhalim , Mohammadi Masoud , Tabarraei Alijan , Hosseini Seyed Mehran 
Published: December 2020

Abstract
Background: Olfactory and gustatory dysfunctions (OD and GD) manifested unpredictably in COVID-19 patients. Considering the high prevalence of OD and GD and the importance of the sense of smell and taste in the quality of life, this study aimed to determine the association of some demographic and clinical factors with OD and GD recovery in COVID-19 patients in Iran.Methods: This prospective cohort study was performed on COVID-19 patients with OD and GD during the first and second peak of pandemic in Golestan province, Iran. We used a local health care registry system for data collection. All cases had positive RT-PCR test for SARS-CoV-19 in nose and nasopharynx swab samples. Variables included age, sex, ethnicity, geographical location, time of onset of symptoms, severity of OD and GD (complete or partial). The follow-up time for recovery from OD and GD was six months. Time to recovery for OD and GD was estimated by Kaplan-Meier considering complete recovery as an endpoint. Differences among groups were evaluated using log-rank test and Cox proportional-hazard models as appropriate in SPSS version 16.Results: A total of 242 patients completed the study. The mean age of patients was 39.12±11.89 years. There were 127 (52%) females. After six months, 239 (98.8%) patients had complete OD recovery and 80.9% and 83.56% of patients recovered within the first 30 days following the onset of OD or GD, respectively. The mean time of OD recovery was significantly higher (35.07±4.25 days) in cases infected in the first peak of pandemic than in the second peak (21.65±2.05 days) (p=0.004). This finding also was observed for the recovery time of GD (p=0.005). The mean recovery time of GD was significantly higher in women than men (35.55±4.33 vs. 23.20±2.46 days) (p=0.04). The age (less than 20 years), second peak of epidemic, and partial dysfunction were identified as significant predictors for recovery.Conclusions: COVID-19–related OD and GD had high rate of recovery in the first month from onset of symptoms. Age of patients, severity of OD/GD and infecting in second peak of epidemic might be related to the recovery of OD or GD in patients with COVID-19.
A guideline for screw fixation of coracoid process base fracture by 3D technology
Authors: Sun Zhongye , Li Hao , Wang Bei , Yan Jun , Han Liren , Han Shizhang , Yang Xiaofei , Zhao Bei 
Published: December 2020

Abstract
BackgroundFractures of the base of the coracoid process are relatively rare, but an increasing number of studies have reported using screws to fix basal coracoid process fractures. This study was performed to simulate the surgical procedure and obtain the ideal diameter, length, insertion point and angle of the screw through the method of 3-D axial perspective in Chinese patients.MethodsWe randomly collected the right scapula computed tomography (CT) scans of 100 adults. DICOM-formatted CT-scan images were imported into Mimics software. The 3D digital model of the right scapula was established. Two virtual cylinders representing two screws were placed from the top of the coracoid process to the neck of the scapula and across the base of the coracoid process to fix the basal coracoid process. The largest secure diameters and lengths of the virtual screws were measured. The positions of the insertion points and the directions of the screws were also researched.ResultsThe screw insertion safe zone can exhibit an irregular fusiform shape from the reconstructed scapula model. The mean maximum diameters of the medial and lateral screws were 7.08 ± 1.19 mm and 7.34 ± 1.11 mm, respectively. The mean maximum lengths of the medial and lateral screws were 43.11 ± 6.31 mm and 48.16 ± 6.94 mm, respectively. The screw insertion corridor with a diameter of at least 4.5 mm was found in anyone. We found gender-dependent differences for the mean maximum diameters and the maximum lengths of the two screws. The positions of the two insertion points were statistically significant in different genders.ConclusionsThe study provides a valuable guideline for the largest secure corridor of two screws that fixed the fracture at the base of the coracoid process. For the ideal screw placement, we suggest an individual preoperative 3D reconstruction simulation. Further biomechanical studies are needed to verify the function of the screws.
Medial Abrasion Syndrome: A Neglected Cause of Persistent Pain After Knee Arthroplasty
Authors: Lyu Shaw-Ruey , Hsu Chia-Chen , Hung Jung-Pin 
Published: December 2020

Abstract
Introduction: Persistent postoperative pain (PPOP) has detracted from some otherwise successful knee arthroplasties. This study investigated medial abrasion syndrome (MAS) as a cause of PPOP after knee arthroplasty. The surgical techniques and outcomes of incorporating this concept into the management of both primary arthroplasty cases and patients suffering from unknown causes of PPOP after arthroplasties were presented. Materials and Methods: In a one-year period, the author performed unicompartmental or total knee arthroplasty (the UKA or TKA group) that also eliminated medial abrasion phenomenon (MAP) on 196 knees of 150 patients at advanced stages of knee osteoarthritis (OA). During the same year, 16 knees of 16 patients with unknown causes of PPOP after knee arthroplasties were referred to the author for the arthroscopic medial release procedure (the AMR group) after being diagnosed as MAS. Subjective satisfaction, Knee Society Score (KSS), and Knee injury and Osteoarthritis Outcome Score (KOOS) evaluations were used for outcome study.Results: All 166 patients were followed for more than 3 years (mean: 3.7 years, 3.1-4.2) for outcome study. All knees receiving arthroplasty showed medial plicae with MAP at the time of surgery. Only 2 of them suffered from PPOP: one was a neglected tibial plateau fracture with residual varus deformity after UKA and the other was a late infection after TKA and received revision. The satisfactory rate was 98.8% in the UKA group, 99.1% in the TKA group, and 100% in the AMR group. The Knee Society scores and all subscales of KOOS were statistically improved in all groups.Conclusions: MAS is a cause of pain in patients who have received knee arthroplasties, and MAP should be eliminated to ensure a successful knee arthroplasty. PPOP after knee arthroplasty can be caused by MAS, which can be managed by AMR.
Genotype-Environment Interaction and Stability&nbsp;of&nbsp;Fiber Properties and Growth Traits&nbsp;in&nbsp;Triploid Hybrid Clones of&nbsp;Populus Tomentosa
Authors: Wu Jian , Zhou Qing , Sang Yaru , Kang Xiangyang , Zhang Pingdong 
Published: December 2020

Abstract
Background: Clones provide a sensitive method for evaluating genotypic stability and detecting genotype-environment (G × E) interactions because of non-additive genetic effects among clones and no genetic effect among ramets of an ortet. The experiments aimed at confirming and expanding the earlier findings, estimating stability parameters, and providing accurate estimates of clonal repeatabilities and genetic gains for an triploid breeding programme of Populus tomentosa Carr. Results: Six 5-year-old clonal trials established in Northern China were used to determine the clonal variation, clone × site interactions and the stability parameters of fiber properties of wood and growth traits. Three hundred sixty trees from ten hybrid clones were collected in the six sites. The clonal and site effects had a highly significant effect (P < 0.001) for all studied traits. The clone × site interactions had a highly significant effect (P < 0.001) on fiber length (FL), coarseness (C), and tree growth (tree height [H], diameter at breast height [DBH] and stem volume [SV]), and a moderate effect (P < 0.05) on fiber width (FW) and fiber length/width (FL/W). For FL and SV, most of the triploid hybrid clones had higher reaction norms to the improvement in growth conditions and higher phenotypic plasticity. The estimated clonal repeatability of FW (0.93) was slightly higher than for FL (0.89), FL/W (0.83), C (0.91), DBH (0.76), H (0.85), and SV (0.80). Three clonal testing sites were sufficient to estimate quantitative parameters of fiber properties. However, more than three clonal testing sites will help improve the accuracy of quantitative parameters of growth traits.Conclusions: Our results unraveled that accurate estimation of quantitative parameters for growth traits in triploid hybrid clones of Populus tomentosa required more clonal testing sites than fiber properties.
WITHDRAWN: Hypothalamic Myo-Inositol and Glucose concentrations, mood symptoms and fatigue in depression: a 3 Tesla chemical-shift-imaging in vivo proton magnetic resonance spectroscopy study
Authors: Pedraz-Petrozzi Bruno , Blecker Carlo , Sammer Gebhard 
Published: December 2020

Abstract
Background: Many studies have confirmed altered brain Myo-Inositol (mIns) and Glucose (Glc) concentrations in depression. Alterations in the mIns-concentrations may represent a glial dysfunction and are associated mostly with depression symptoms. In comparison, alterations in the Glc-concentrations could represent impaired glucose metabolism. Despite this information, studies that assess these metabolites in the hypothalamus are missing. Therefore, this study aims to investigate mIns- and Glc-concentrations in the hypothalamus of participants with depression concerning mood symptoms and fatigue.Methods: We performed an in vivo proton magnetic resonance spectroscopy – chemical shift imaging of the hypothalamus in 49 participants (25 with depression diagnosis or DE, 24 healthy controls or HC), evaluating mIns- and Glc-concentrations. Concerning information about symptoms, we recollected information on depression symptomatology (BDI-FSTOT) and fatigue (FIS-D). For statistical analysis, we run generalized linear models by using the Poisson regression model with count data.Results: The results showed reduced hypothalamic mIns-concentration for every DE participant with higher FIS-D and higher BDI-FSTOT values (OR = 0.022; p < 0.001; CI95 [0.004 to 0.09]). Besides, the results showed increased hypothalamic Glc-concentration for every participant of the DE group and with higher BDI-FSTOT values (OR = 4.827; p = 0.002; CI95 [1.826 to 13.749]).Conclusions: These results provide initial evidence of a glial dysfunction and impaired glucose metabolism in the hypothalamus associated with mood symptoms and fatigue. Future studies are needed to confirm these pathophysiologic mechanisms.
SPP1&nbsp;Might Be a Novel Prognostic Biomarker for Patients With Malignancy: a Meta-analysis and Sequential Verification Based on Bioinformatic Analysis
Authors: Zheng Hao-ran , Jiang Ai-Min , Liu Na , Ding Qian-Qian , Zhao Fu-Mei , Fu Xiao , Liang Xuan , Tian Tao , Ruan Zhi-Ping , Yao Yu 
Published: December 2020

Abstract
Background: Several studies have investigated the relationship between secreted phosphoprotein 1 (SPP1) expression level and prognosis of various tumors, but the results are far from conclusive. Therefore, we performed the present meta-analysis to investigate the prognostic value of SPP1 in pan-cancer. Furthermore, a followed confirmation based on The Cancer Genome Atlas (TCGA) database was also performed to verify our results.Methods: We performed a systematic search from PubMed, Embase, Web of Science, and Cochrane Library databases and 19 articles, including 3403 patients and 9 types of tumors, were pooled in our meta-analysis. Overall survival (OS) and disease-free survival (DFS), which correlated with SPP1 expression, were considered as the primary outcome. Subgroup analyses, sensitivity analysis, and publication bias were used to investigate heterogeneity and reliability of the results. Furthermore, we also explored the relationship between SPP1 expression and clinical parameters of tumor patients. Finally, the results were verified with TCGA database and we further explored the relationship between SPP1 expression and tumor immuno-microenvironment (TIME), DNA methylation, and enriched gene pathway.Results: Our meta-analysis showed that high-expressed SPP1 was significantly related to poor OS and DFS in various cancers, especially in liver hepatocellular carcinoma (LIHC). Furthermore, we also identified that the high expression level of SPP1 was significantly correlated with tumor grade. The expression level of SPP1 in the majority of tumor types were much higher than the corresponding normal tissues analyzed from databases. Besides, we also observed that high-expressed SPP1 was related to poor OS and DFS in LIHC, which supported the conclusion of meta-analysis. In addition, high-expressed SPP1 is related to 6 immune cells in TIME and DNA methylation regulatory genes. Ultimately, the results of Gene Set Enrichment Analysis (GSEA) suggested that tumor-related gene sets, such as hypoxia and lipid metabolism, were significantly enriched in high-expressed SPP1 group.Conclusions: SPP1 is high-expressed in various tumor tissues and correlated with poor prognosis. SPP1 might promote cancer invasion and metastasis by affecting tumor grade, TIME, DNA methylation, hypoxia, and lipid metabolism. SPP1 is expected to become a new clinical indicator for tumor detection and prognosis, and provide a new idea for tumor targeted therapy.
Identification of Metabolic Reprogramming Related Gene Signature to Predict the Prognosis of Bladder Cancer Patients
Authors: Li Tinghao , Tong Hang , Yin Hubin , Cao Honghao , Zhu Junlong , Qin Zijia , Yin Siwen , He Weiyang 
Published: December 2020

Abstract
Background: Different kinds of metabolic reprogramming have been widely researched in multifarious cancer types and show up as a guaranteed prognostic predictor, while bladder cancer (BLCA) is most frequent urothelium carcinoma but with poor prognosis despite there are emerging treatments, for lack of reliable predicting biomarkers to early predict the prognosis and delayed treatment options for patients in the terminal stage. Our study aims to explore new prognostic factors related to metabolism in BLCA and make these genes up as novel risk stratification.Methods: We selected a large number of samples downloaded from TCGA (The Cancer Genome Atlas) to find out the possible glycolysis-related genes that correlated with differentiation from cancer sample to normal tissue, aimed to find out a more credible model. To make our signature more believable, we chose the clinical features information from GEO (Gene Expression Omnibus) database as external validation cohort.Results: Finally, we established a three glycolysis-related gene signature based on the expression of AK3, GALK1 and NUP205 to make a prediction on the prognosis of BLCA patients, which were also validated by external cohort and whole mixed cohort. As a result, we built a three glycolysis-related gene signature and found its prognosis value is more valuable in high malignancy patients, which may help physicians to make a more aggressive choice.
JKAMP Inhibits the Osteogenic Capacity of Adipose-derived Stem Cells in Diabetic Osteoporosis by Modulating the Wnt Signaling Pathway Through Intragenic DNA Methylation
Authors: Peng Shuanglin , Shi Sirong , Tao Gang , Li Yanjing , Xiao Dexuan , Wang Lang , He Qing , Cai Xiaoxiao , Xiao Jingang 
Published: December 2020

Abstract
Background: Diabetic osteoporosis (DOP) is a systemic metabolic bone disease caused by diabetes mellitus (DM). Adipose-derived stem cells (ASCs) play an important role in bone regeneration. Our previous study confirmed that ASCs from DOP mice (DOP-ASCs) have a lower osteogenesis potential compared with control ASCs (CON-ASCs). However, the cause of this poor osteogenesis has not been elucidated. Therefore, this study investigated the underlying mechanism of the decline in the osteogenic potential of DOP-ASCs from the perspective of epigenetics and explored methods to enhance their osteogenic capacity. Methods: The expression level of JNK1-associated membrane protein (JKAMP) and degree of DNA methylation in CON-ASCs and DOP-ASCs were measured by mRNA expression profiling and MeDIP sequencing, respectively. JKAMP small interfering RNA (siRNA) and a Jkamp overexpression plasmid were used to assess the role of JKAMP in osteogenic differentiation of CON-ASCs and DOP-ASCs. Immunofluorescence, qPCR, and western blotting were used to measure changes in expression of Wnt signaling pathway-related genes and osteogenesis-related molecules after osteogenesis induction. Alizarin red and ALP staining was used to confirm the osteogenic potential of stem cells. Bisulfite-specific PCR (BSP) was used to detect JKAMP methylation degree. Results: Expression of JKAMP and osteogenesis-related molecules (RUNX2 and OPN) in DOP-ASCs was decreased significantly in comparison with CON-ASCs. JKAMP silencing inhibited the Wnt signaling pathway and reduced the osteogenic ability of CON-ASCs. Overexpression of JKAMP in DOP-ASCs rescued the impaired osteogenic capacity caused by DOP. Moreover, JKAMP in DOP-ASCs contained intragenic DNA hypermethylated regions related to the downregulation of JKAMP expression. Conclusions: Intragenic DNA methylation inhibits the Wnt signaling pathway by suppressing expression of JKAMP and the osteogenic ability of DOP-ASCs. This study shows an epigenetic explanation for the reduced osteogenic ability of DOP-ASCs, and provides a potential therapeutic target to prevent and treat osteoporosis.