bluuebunny/crossref_metadata_2025_split huggingface dataset:
Propofol Stimulates Immune Activity and Decreases Inflammatory Cytokines via NF-κB-Mediated JAK1-STAT3 Pathway in Gastric Cancer Patients Undergoing Radical Surgery
Authors: Zhang Haili , Qian Shi , Jing Wang 
Published: August 2020

Abstract
BackgroundPropofol is the most commonly used general anesthesia for patients with gastric cancer undergoing radical surgery. Studies have suggested that propofol exerts beneficial effects on the immune function of patients with cancer. However, the potential mechanism underlying propofol-mediated immune regulation remains to be elucidated. The present study investigated the regulatory effects of propofol on immune function in patients with gastric cancer undergoing radical surgery. MethodsELISA, reverse transcription-quantitative polymerase chain reaction, western blotting, gene transfection and immunohistochemistry were used to analyze the effects of propofol on gastric cancer cells.ResultsResults demonstrated that propofol general anesthesia resulted in an increased percentage of cluster of differentiation (CD)4+ and CD8+ cells, increased serum concentrations of interleukin (IL)-2 and tumor necrosis factor (TNF)-α, decreased serum concentrations of IL-1β and IL-8 following propofol general anesthesia in gastric cancer patients undergoing radical surgery compared with midazolam. In addition, propofol general anesthesia induced an imbalance in T helper (Th)1/Th2 cells, increased the number of natural killer cells and B cells, decreased the expression of prognostic factors, and improved tumor metastasis, recurrence and survival in patients with gastric cancer compared with midazolam. Furthermore, immunohistochemistry demonstrated that propofol downregulated nuclear factor (NF)-κBp65, and upregulated Janus kinase 1 (JAK1) and signal transducer and activator of transcription 3 (STAT3) expression level in gastric cancer tissues, downregulated the protein expression levels of NF-κBp65, JAK1, STAT3, TNF-α, IL-1β and IL-8 protein expression in gastric cancer cells isolated from gastric cancer tissues. NF-κBp65 overexpression inhibited propofol-mediated upregulation of JAK1, STAT3, TNF-α, IL-1β and IL-8 expression in gastric cancer cells. ConclusionsThese data indicate that that propofol may increase the number of T cells, stimulate T-cell proliferation, upregulate IL-2 and TNF-α expression, and enhance immune function via the NF-κB-mediated JAK1-STAT3 signaling pathway in patients with gastric cancer undergoing radical surgery.
Small dense low-density lipoprotein cholesterol is an effective target marker for predicting cardiovascular events and laser treatment for retinopathy in diabetic patients
Authors: Nakayama Atsuko , Morita Hiroyuki , Sato Tatsuyuki , Kawahara Takuya , Takeda Norifumi , Kato Satoshi , Itou Hiroshi , Komuro Issei 
Published: August 2020

Abstract
Background

Small dense low-density lipoprotein cholesterol (sdLDL-C) has been recently reported as a sensitive marker for cardiovascular diseases.
Objective

We explored the superiority of sdLDL-C as a marker for predicting cardiovascular (CV) events and the need for laser treatment in patients with hypercholesterolemia and diabetic retinopathy.
Methods

We performed a sub-analysis of the intEnsive statin therapy for hyper-cholesteroleMic Patients with diAbetic retinopaTHY (EMPATHY) study (n = 5042), in which patients were assigned randomly to intensive or standard statin therapy targeting low-density lipoprotein cholesterol < 70 mg/dl or 100–120 mg/dl. Using the Cox hazard analysis and Kaplan-Meier analysis, the risks for CV events and the need for laser treatment were evaluated according to the following lipids: total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglycerides, apolipoprotein A1, apolipoprotein B (ApoB), and sdLDL-C one year after registration.
Results

The patients were 63 ± 11 years old and 48% of them were male. LDL-C and sdLDL-C levels were 98 ± 25 and 32 ± 14 mg/dl, respectively, one year after registration. The sdLDL-C level had a strong positive correlation with ApoB level (r = 0.83). SdLDL-C was a sensitive marker for predicting CV events when comparing among the quartiles according to sdLDL-C levels (hazard ratios: HR for quartiles 1–4 were 1.0, 1.4, 1.6, and 2.5, respectively; p for trend < 0.01). Also, sdLDL-C was a sensitive marker for predicting the need for laser treatment among lipids (log rank, p = 0.009), especially in patients with elderly (> 65 yrs) and obesity (BMI > 25 kg/m2).
Conclusions

SdLDL-C is a sensitive target marker to predict cardiovascular events as well as the need for laser treatment in patients with hypercholesterolemia and diabetic retinopathy.
Trial registration:
UMIN000003486, www.umin.ac.jp/ctr/.
Association of AGT rs2493132 Polymorphism and the Risk of Coronary Artery Disease in Patients with Non-alcoholic Fatty Liver Disease in Chinese Han Population
Authors: Dong Mengzhen , Zhang Jie , Ma Xuefeng , Wang Mengke , Wang Yifen , Liu Shousheng , Yongning Xin 
Published: August 2020

Abstract
Background: Previous studies proved that AGT gene polymorphisms were associated with the serum lipids metabolism; however, it is not clear whether AGT rs2493132 increases the risk of NAFLD and CAD. This study was designed to investigate the relationship between AGT rs2493132 gene polymorphism and the developing risk of NAFLD and CAD in Chinese Han population.Methods: A total of 142 NAFLD patients, 109 NAFLD + CAD patients, and 246 health controls were included in this study. PCR method was used to measure AGT genotype. The anthropometric and clinical data were measured in clinical laboratory department of Qingdao Municipal Hospital. Statistical analyzes were conducted using the SPSS statistical software, version 21.0. Results: The AGT rs2493132 CT + TT genotype was an important risk factor for CAD in patients with NAFLD (OR = 1.785; 95%CI: 1.061-3.003; P = 0.029), and the risk of CAD in patients with NAFLD was still marked after adjusted for gender, age, and BMI (OR = 4.718; 95%CI: 1.502-14.824; P = 0.008). The T allele of AGT rs2493132 also significantly increased the risk of CAD in NAFLD patients (OR = 2.436; 95% CI: 1.184-5.011, P = 0.016) after adjusted for age, gender, and BMI. In addition, AGT rs2493132 T allele carriers possess the higher TC and LDL levels compared to the non-carriers in all the participants (Both P < 0.05).Conclusions: AGT rs2493132 CT + TT genotype and T allele significantly increased the developing risk of CAD in the patients with NAFLD in Chinese Han population. AGT rs2493132 T allele associated with the increased serum TC and LDL levels.
Changes in psychological distress and quality of life after esophageal cancer surgery: A prospective study
Authors: Kim Jeong Hye , Jung Ju Ri 
Published: August 2020

Abstract
Background

Esophageal cancer patients experience physical and psychological difficulties after surgery. This study aimed to identify the changes in psychological distress and quality of life in patients with esophageal cancer before surgery to three months after surgery.
Methods

We enrolled 49 patients who were scheduled to undergo esophageal surgery at a tertiary hospital in Seoul, South Korea in this prospective study. Patients’ psychological distress and quality of life were assessed with the Korean scales HADS, EORTC QLQ-C30, and QLQ-OES18 at the pre-surgery, one-month post-surgery, and three months post-surgery.
Results

Moderate-to-severe depression was reported in 12.2% of patients at the pre-surgery evaluation, in 57.1% of patients one-month post-surgery, and 8.2% of patients three-months post-surgery. Moderate-to-severe depression was reported in 12.2% of patients at the pre-surgery evaluation, in 63.3% of patients one-month post-surgery, and 16.3% of patients three months post-surgery. Clinically significant, moderate changes (10–20 points) in physical functioning, insomnia, nausea and vomiting, and dyspnea, and significant, large changes (> 20) in role functioning, fatigue, pain, and appetite loss (per EORTC QLQ-C30) were reported from pre-surgery to one-month post-surgery. Clinically significant, moderate changes (10–20 points) in dysphagia and taste problems and a significant, large change (> 20) in eating difficulties (per QLQ-OES18) were reported from pre-surgery to one-month post-surgery.
Conclusion

One month after esophageal cancer surgery, patients demonstrated severe psychological distress and worsening quality of life.
Integrated transcriptome and proteome analysis provides insights into leaf color differences between red-leaved poplar cultivars (‘Quanhong’ and ‘Xuanhong’) and ‘Zhonglin 2025’ (Populus deltoides cv. ‘Zhonglin 2025’)
Authors: Chen Xinghao , Liu Hanqi , Wang Shijie , Zhang Chao , Yang Minsheng , Zhang Jun 
Published: August 2020

Abstract
BackgroundThe red-leaved poplar cultivars ‘Quanhong’ and ‘Xuanhong’ are bud mutations of Populus deltoides cv. ‘Zhonglin 2025’. These cultivars are valued for their beautiful shape, lack of flying catkins, and ornamental leaf colors. The molecular mechanism that leads to different leaf colors between these red-leaved poplars and ‘Zhonglin 2025’ remains unclear.ResultsIn this study, we analyzed growth and physiological indicators in the ‘Quanhong’, ‘Xuanhong’, and ‘Zhonglin 2025’ poplar cultivars, and performed transcriptome and proteome analysis of their leaves. The results showed that plant height and ground diameter were significantly lower in both red-leaved poplars than in ‘Zhonglin 2025’, indicating that their growth and development were markedly inhibited. The ratio of anthocyanin to total chlorophyll and photosynthetic capacity of the leaves were higher and lower, respectively, in the red-leaved cultivars than in ‘Zhonglin 2025’. At the transcript and protein levels, 6,792 differentially expressed genes (DEGs) and 2,786 differentially expressed proteins (DEPs) were screened in the ‘Quanhong’ cultivar, respectively, and 4,398 DEGs and 2,333 DEPs were screened in the ‘Xuanhong’ cultivar, respectively. We screened 769 DEGs/DEPs in ‘Quanhong’ and 399 DEGs/DEPs in ‘Xuanhong’ in an integrated transcriptomics/proteomics analysis. Based on the results of this integrated analysis, 15 and 11 genes/proteins involved in anthocyanin synthesis were further identified in ‘Quanhong’ and ‘Xuanhong’, respectively, including the CHS , F3H , and DFR genes. Among the 120 transcription factors, 3 (HY5, HYH, and TTG2), may be directly involved in the regulation of anthocyanin synthesis in both red-leaved poplars.ConclusionsBy comparing the proteomes and transcriptomes of red-leaved poplar cultivars and ‘Zhonglin 2025’, we identified the key genes and proteins related to red leaf coloration. The findings of this study provided insights that may aid further studies of the molecular mechanism of leaf red coloration in red-leaved poplars.
Correlation between perioperative parecoxib and postoperative acute kidney injury in patients undergoing non-cardiac surgery: a retrospective cohort analysis
Authors: Tang Yongzhong , Zeng Pingping , Liao Yan , Qin Zheng , Zhang Hao , Li Bo , Ouyang Wen , Li Dan 
Published: August 2020

Abstract
Background: The association of nonsteroidal anti-inflammatory drugs with postoperative acute kidney injury is controversial. However, there are few studies focusing on the association between parecoxib and postoperative acute kidney injury. Methods: We retrospectively reviewed the electronic medical records and laboratory results of 9,246 adult patients (18–60 years) undergoing non-cardiac surgery at Third Xiangya Hospital of Central South University from January 1, 2012 to August 31, 2017. Study groups were either treated with or without parecoxib. Univariable analysis identified demographic, preoperative laboratory, and intraoperative factors associated with acute kidney injury. Logistic stepwise regression was used to calculate the adjusted odds ratio of parecoxib and acute kidney injury association. Results: The incidence of postoperative acute kidney injury was 6.06% and parecoxib was used in 0.105% of patients. The mortality was 4.64% in the acute kidney injury group. The incidence of acute kidney injury was lower in the parecoxib-administered group (4%) than in the without parecoxib-administered group (6.3%, p = 0.005). Postoperative acute kidney injury risk reduced by 33.40% in the parecoxib-administered group after adjusting for interference factors.Conclusions: Intraoperative single-dose parecoxib (40 mg or 80 mg) might reduce postoperative acute kidney injury risk in adult patients undergoing non-cardiac surgery.
Tet2 inactivation enhances the anti-tumor activity of tumor-infiltrating lymphocytes (TILs) to curtail melanoma growth
Authors: Lee Minjung , Li Jianfang , Fang Shaohai , Zhang Joanna , Vo Anh , Han Wei , Zeng Hongxiang , Isgandarova Sevinj , Martinez-Moczygemba Margarita , Han Weidong , Zhou Yubin , Li Jia , Sun Deqiang , Huang Yun 
Published: August 2020

Abstract
Inactivation of tumor infiltrating lymphocytes (TILs) is one of the mechanisms mitigating anti-tumor immunity during tumor onset and progression. Epigenetic abnormalities are regarded as a major culprit contributing to the dysfunction of TILs within tumor microenvironments. In this study, we used a murine model of melanoma to discover that Tet2 inactivation significantly enhances the anti-tumor activity of TILs, with the efficacy comparable to immune checkpoint inhibition imposed by anti-PD-L1 treatment. Single-cell RNA-seq analysis further revealed that Tet2-deficient TILs exhibit effector-like features. Transcriptomic and ATAC-seq analysis further demonstrated that Tet2 deletion reshapes the chromatin accessibility and favors the binding of transcription factors geared toward CD8+ T cell activation. In summary, our study establishes that Tet2 constitutes one of the epigenetic barriers contributing to dysfunction of TILs, and that Tet2 inactivation could benefit anti-tumor immunity to boost tumor suppression.
A plasma membrane protein based prognostic model in clear cell renal cell carcinoma
Authors: Liu Haosheng , Fang Jianxiong , Liu Tianqi , Zhang Zhenhui , Zhao Chao , Peng Tianming , Li Yong , Pu Xiaoyong , Liu Jiumin 
Published: August 2020

Abstract
Background

Renal cell cacinoma (RCC) accounts for 3% of human cancers, and clear cell renal cell carcinoma (ccRCC) is the most common pathological type of RCC. Cell surface proteins have been shown to play an important role in the occurrence and progression of various cancers. In this study, we focused on plasma membrane proteins (PMPs), to explore their potential value in ccRCC.
Methods

The PMPs expression profiles and ccRCC patients’ clinical information were downloaded from TCGA database. Through a series of bioinformatic methods, we established a plasma membrane proteins prognostic model.
Results

Multivariate cox regression analysis and area under receiver operating characteristic curve indicated that this model was an effective independent predictor of ccRCC clinical outcomes. Combined with other two clinical characteristics, a nomogram was constructed to predict patient survival at 1, 3, and 5 years.
Conclusions

Our study is the first to explore the prognostic value of plasma membrane proteins in clear cell renal cell carcinoma. We hope our work could provide a new viewpoint for ccRCC prognosis and drawn people’s attention to plasma membrane proteins in clear cell renal cell carcinoma.
Associations Between Symptoms and Quality of Life in Lumbar Degenerative Disease and Cognitive Dysfunction in a Japanese Community: A Cross-sectional Study
Authors: Koyama Kazushige , Wada Kanichiro , Kumagai Gentaro , Kudo Hitoshi , Tanaka Sunao , Asari Toru , Ihara Kazunari , Nakaji Shigeyuki , Ishibashi Yasuyuki 
Published: August 2020

Abstract
Background: Lumbar degenerative disease (LDD) and dementia are increasing in super-aging societies and are both related to physical dysfunction and pain. However, the relationship between these diseases remains unclear. This cross-sectional study aimed to investigate the rate of comorbidity of lumbar spinal stenosis (LSS) and mild cognitive impairment (MCI) by age and sex and clarify the association between LSS presence, LDD symptoms, quality of life (QOL) related to low back pain, and cognitive impairment in a Japanese population.Methods: We enrolled 1097 participants (men 437; women 660; mean age 54.6 years) from a medical checkup program. LSS was diagnosed using a self-administered questionnaire, and LDD symptoms were evaluated using visual analog scale (VAS: low back pain, pain, numbness of the lower limb). QOL related to low back pain was evaluated using the Japanese Orthopedic Association Back-Pain Evaluation Questionnaire (JOABPEQ: pain, lumbar function, and gait function), and Kellgren-Lawrence grading was performed to evaluate lumbar degeneration using lateral radiographs of the lumbar spine. Cognitive function was measured using the Mini Mental State Examination (MMSE), and we defined MCI as a summary score of MMSE £27. Logistic and multiple liner regression analyses were performed to analyze the association between MCI, summary score of MMSE and LSS factors.Results: The comorbidity rate of MCI and LSS was 0.9% in men and 0.7% in women. It was 2.1% in those aged ³65 years in both sexes, and this rate increased with age. Lumbar function in JOABPEQ (OR 0.979, 95% CI 0.961 to 0.998) and lower limb pain in VAS (OR 1.020, 95% CI 1.002 to 1.039) were associated with MCI in men. The presence of LSS, lumbar function, and gait function in JOABPEQ were associated with MMSE in both sexes, while lower limb numbness and pain in VAS were associated with MMSE in men.Conclusion: The comorbidity rate of LSS and MCI increased with age. The presence of LSS, certain symptoms of LDD, and deterioration of QOL due to low back pain were related to cognitive dysfunction. Treatment of LDD could help to improve both LDS symptoms and cognitive dysfunction.
Association of XRCC1 rs1799782 and ERCC2 rs13181 Polymorphisms with Glioma Risk: A Systematic Review and Meta-Analysis
Authors: Tavares Cléciton Braga , Alves-Ribeiro Francisco Adelton , Junior Elmo de Jesus Nery , Vasconcelos-Valença Rodrigo Jose de , Campos-Verdes Larysse Cardoso , Gomes-Braga Francisca das Chagas Sheyla Almeida , Lopes-Costa Pedro Vitor , Santos Alesse Ribeiro dos , Pinho-Sobral André Luiz , Campelo Viriato , Cardoso Arquimedes Cavalcante , Sousa Emerson Brandao , Pereira Renato de Oliveira , Gebrim Luiz Henrique , Silva Benedito Borges da 
Published: August 2020

Abstract
BackgroundGliomas are the most common primary tumors of the central nervous system with unclear etiology, however hereditary factors may play important roles in the development of gliomas with mutations and single nucleotide polymorphisms (SNPs) being prominent among the genetic changes. this systematic review and meta-analysis assess the association of XRCC1 (rs1799782) and ERCC2 (rs13181) gene polymorphisms and glioma risk.MethodsThis study included articles indexed in the PUBMED and EMBASE databases published during the past 15 years. Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were followed. The PICOS model was used to develop the inclusion criteria and search terms. This review was recorded at PROSPERO International prospective register of systematic reviews, ID 196173. The META-MAR V2.7.0 meta-analysis calculator was used for the statistical analysis with p-values < 0.05 being considered statistically significant. Dichotomous data are presented as odds ratios (OR) with a 95% confidence interval (CI). Statistical heterogeneity was measured using the I2 test and I2 > 50% were regarded as high heterogeneity. Funnel plots, Begg (BT) and Egger Tests (ET) were used to assess publication bias (p<0.1). Results Literature review identified 10 articles on ERCC2 gene rs13181 variant and 11 on XRCC1 rs1799782. The meta-analysis identified a risk for gliomas for the TT genotype of the XRCC1 rs1799782 SNP in Asians (OR: 1.59, 95% CI: 1.3-1.93; p=0.006; I2 13.1%). ERCC2 rs13181 polymorphisms identified as risks for gliomas were AC genotypes in Asians (OR: 2.06, 95% CI: 1.75-1.42; p = 0.00057; I2 91.1%) and Caucasians (OR: 1.16, 95% CI: 1.01-1.31; p = 0.02; I2 12.2%), and CC genotypes in Caucasians (OR: 2.06, 95% CI: 1.75-1.42; p = 0.0001; I2 98.9%).Conclusions TT genotypes of the XRCC1 rs1799782 SNP in Asians, AC genotypes of ERCC2 rs13181 polymorphisms in Asians and Caucasians, and CC genotypes of ERCC2 rs13181 polymorphisms in Caucasians were associated with increased risk for gliomas that may benefit these patients with early diagnostic and therapeutic strategies.
Plasma D-Dimer Level is Associated with Clinical Outcomes in Patients with Atrial Fibrillation Related Acute Ischemic Stroke after Pneumonia
Authors: Yang Xu , Lu Taoli , Qu Zhanli , Zhang Yi , Liu Pingping , Ma Ying 
Published: August 2020

Abstract
Objectives: Pneumonia is related to poor prognosis in acute ischemic stroke (AIS), and its risk might be higher in atrial fibrillation (AF) related AIS with elevated plasma D-dimer. The aim of our study was to investigate the prognostic value of D-dimer for predicting clinical outcome of AF-related AIS with pneumonia. Method: AF-related AIS patients with pneumonia were prospectively enrolled. Receiver operating characteristic (ROC) curve was used to determine the optimal D-dimer point for 3-month mortality and death/severe disability. The associations between the D-dimer and 3-month mortality and death/severe disability were assessed by multivariable logistic regression analysis. Results: A total of 415 patients were enrolled in this study. ROC curve analysis showed that the optimal cut point of D-dimer for 3-month death/severe disability and mortality were D-dimer≥2.35mg/l and D-dimer≥3.35mg/l, respectively. Multivariable logistic regression analysis showed that D-dimer≥2.35mg/l(OR 5.95, 95% CI: 3.09-11.45, P＜0.001), higher NIHISS score(OR:1.53, 95% CI: 1.38–1.68, P＜0.001) were associated with increased risk of 3-month death/severe disability), and anticoagulant was associated with decreased risk of death/severe disability (OR:0.16, 95% CI: 0.07-0.35, P＜0.001). Higher NIHISS score(OR:1.56, 95% CI: 1. .37–1.77, P＜0.001), higher age(OR 1.06, 95% CI: 1.01–1.11, P=0.019), D-dimer≥3.35mg/l (OR 7.85, 95% CI: 3.21-19.21, P=0.001)were associated with increased risk of 3-month mortality.Conclusions: AF-related AIS patients with concurrent high D-dimer and pneumonia increased risk of 3-month mortality and death/severe disability, plasma D-dimer may have predictive value in outcome after AF-related AIS with pneumonia.