bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid Versus Laboratory-Based Testing for HIV and Hepatitis C at a Drug Detoxification Treatment Center: A Randomized Trial
Authors: Assoumou Sabrina A , Paniagua Samantha M , Linas Benjamin P , Wang Jianing , Samet Jeffrey H , Hall Jonathan , White Laura F , Beckwith Curt G 
Published: April 2020

Abstract

Background
A health department survey revealed nearly half employ laboratory-based HIV and HCV testing (LBT) over rapid testing (RT) in nonhospital settings such as drug detoxification centers. LBT has higher sensitivity for acute HIV infection compared to RT but LBT is not point of care and may result in fewer diagnoses due to loss to follow-up before result delivery.

Methods
We conducted a randomized trial comparing real-world case notification of RT (Orasure) vs LBT (HIV Combo Ag/Ab EIA, HCV EIA) for HIV and HCV at a drug detoxification center. Primary outcome was receipt of test results within 2 weeks.

Results
Among 341 individuals screened (11/2016–7/2017), 200 met inclusion criteria; 58% injected drugs and 31% shared needles in the previous 6 months. Of the 200 randomized, 98 received RT and 102 LBT. Among all participants, 0.5% were positive for HIV and 48% for HCV; 96% received test results in the RT arm and 42% in the LBT arm (odds ratio, 28.72; 95% confidence interval, 10.27–80.31). Real-world case notification was 95% and 93% for HIV and HCV RT, respectively, compared to 42% for HIV and HCV LBT.

Conclusions
RT has higher real-world case notification than LBT at drug detoxification centers.
Clinical trials registration: NCT02869776.
Biochemical properties of human full-length aryl hydrocarbon receptor (AhR)
Authors: Uemura Seiya , Nakajima Yasutomo , Yoshida Yuhki , Furuya Moeko , Matsutani Shun , Kawate Shinya , Ikeda Shun-ichi , Tsuji Noriko , Grave Ewa , Wakui Hideki , Itoh Hideaki 
Published: April 2020

AbstractThe aryl hydrocarbon receptor (AhR) is a very unstable protein. AhR binds to the molecular chaperone complex (HSP90-p23-XAP2) to maintain a stable structure in the cytoplasm. After binding to ligands, such as dioxin, AhR translocates from the cytoplasm to the nucleus with a molecular chaperone complex. The protein forms a heterodimer with Arnt after nuclear transfer, functions as a transcription factor by binding to a xenobiotic responsive element (XRE), and induces the cytochrome P450 1A1 (CYP1A1). Because of the unstable protein, expression of the full-length AhR in the E. coli expression system is very difficult. Many studies investigated AhR using AhR domains in vitro. We expressed and purified the human full-length AhR in E. coli expression system. Furthermore, specific antibodies were prepared. Purified full-length AhR could bind to ligand. In the presence of ligand, α-helix and random coil of AhR increased and β-sheet decreased on CD spectrum. Full-length AhR could bind to HSP90, XAP2 and p23 in the presence or absence of ligand. We now show the biochemical properties of full-length AhR.
Use of electroencephalogram and long short-term memory networks to recognize design preferences of users toward architectural design alternatives☆
Authors: Chang Sunwoo , Dong Wonhyeok , Jun Hanjong 
Published: April 2020

Abstract
In this study, we propose an electroencephalogram (EEG)-based long short-term memory networks model for recognizing user preferences toward architectural design images. An EEG is an approach that records the electrical activity in the brain, and EEG-based affection recognition is a technique used for quantitatively recognizing human emotion by analysing the recorded signals. Decision-makers’ subjective reactions toward architectural design alternatives may play a key role in the architectural planning and design stage. In this regard, the proposed model enables the quantitative recognition of their preferences and supports architects in the planning and design stages. The suggested model classifies the recorded data using a deep-learning technique. To build the model, an EEG recording experiment was conducted with 18 subjects, who were asked to select their most/least preferred images among eight images of small-housing design. Post recording, a positive and negative affect schedule questionnaire was distributed to the subjects to rate their affection. Google TensorFlow and Keras were used to structure the model. After training, precision, recall, and f1 score metrics were used to evaluate and validate the model. This model can help designers to evaluate design alternatives in terms of decision-making. Moreover, as this model uses biosignal data, which is universal to humans, architectural design processes for children, the elderly, etc., may be supported. Furthermore, a data-driven design database may be proposed in a future research for cross-validating with previous methods such as interviews and observations.
Improved Sentinel Method for Surveillance and Collection of Filth Fly Parasitoids
Authors: Geden Christopher J , Johnson Dana M , Taylor David B 
Published: April 2020

Abstract
Parasitoids are important natural enemies of house flies and other muscoid flies. The two most commonly used methods for collecting fly parasitoids from the field have distinct advantages and disadvantages. Collections of wild puparia depend on the ability to find puparia in sufficient numbers and are prone to localized distortions in relative species abundance because of the overrepresentation of samples from hot spots of fly larval activity. Placement and retrieval of sentinel puparia is convenient and allows consistent sampling over time but is strongly biased in favor of Muscidifurax spp. over Spalangia spp. An improved sentinel method is described that combines some of the advantages of these two methods. Fly medium containing larvae is placed in containers, topped with a screen mesh bag of puparia, and placed in vertebrate-proof wire cages. Cages are placed at sites of actual or potential fly breeding and retrieved 3–7 d later. The modified method collected species profiles that more closely resembled those of collections of wild puparia than those from sentinel pupal bags. A method is also described for isolating puparia individually in 96-well tissue culture plates for parasitoid emergence. Use of the plate method provided a substantial saving of time and labor over the use of individual gelatin capsules for pupal isolation. Puparia from the collections that were housed individually in the wells of tissue culture plates had a higher proportion of emerged Spalangia species than puparia that were held in groups.
Rhetorical strategies of legitimation in the professional field of banking
Authors: Siebert Sabina , Martin Graeme , Simpson Gavin 
Published: April 2020

AbstractIn this study, we analyse the rhetorical strategies of legitimation used by professionals when their conduct is exposed as wrong. Focusing on banking as a professional field and the conduct of bankers during the 2007–8 global financial crisis we ask two questions: what rhetorical strategies did senior bankers use to justify their actions and defend the legitimacy of their profession in the face of widespread public disapproval of banking practices? How did bankers use their professional field to legitimize their behaviour? To answer these questions, we analyse the justificatory rhetoric used by UK banking executives during the Treasury Select Committee hearings following the crisis. Drawing on our analysis we developed a typology of rhetorical strategies of legitimation used by the bankers, based in part on the concept of neutralization techniques. We argue that bankers, with some exceptions, drew largely on intra-field rhetoric, deeply embedded in institutionalized practices, to justify their behaviour and legitimize their profession. The lack of more convincing inter-field rhetoric only accentuated the mismatch between the moral universe of the bank executive and that of the traditional citizen, voter, and taxpayer.
Stereotactic body radiation therapy for early-stage non–small-cell lung cancer in octogenarians and older: an alternative treatment
Authors: Bei Yanping , Murakami Naoya , Nakayama Yuko , Okuma Kae , Kashihara Tairo , Raturi Vijay Parshuram , Okamoto Hiroyuki , Takahashi Kana , Inaba Koji , Igaki Hiroshi , Itami Jun 
Published: April 2020

ABSTRACT
Surgery is the standard modality for early-stage I–II non-small-cell lung cancer (NSCLC). Generally, patients who are &gt;80 years old tend to have more comorbidities and inferior physical status than younger patients. Stereotactic body radiation therapy (SBRT) may provide an alternative treatment for this group of patients. Here, we report our experience using SBRT to in the management of early-stage NSCLC in patients &gt;80 years old. Patients aged ≥80 years old who were diagnosed with early-stage NSCLC and treated with definitive lung SBRT from January 2000 to January 2018 were retrospectively analysed. Local recurrence-free survival (LRFS), regional recurrence-free survival (RRFS), cancer-specific survival (CSS), progression-free survival (PFS), overall survival (OS) and treatment-related toxicities were analysed for patients &gt;80 years old. A total of 153 patients were included, with a median age of 85 years (range, 80–94). The median follow-up period and OS was 39.8 months (range, 10–101 months) and 76 months, respectively. The 3-year OS, PFS, CSS, RRFS and LRFS were 65.3, 58.0, 75.7, 73.9 and 85.3%, respectively. Radiation pneumonitis grade 0–1, grade 2, grade 3 and grade 4 was observed in 135 (88.2%), 13 (8.5%), 4 (2.61%) and 1 (0.6%) patient(s), respectively. On multivariate analyses, tumor size, pretreatment C-reactive protein (CRP) value, histology and pretreatment physical state were significantly associated with OS. Definitive lung SBRT appears to have high LRFS and OS without causing high-grade radiation-related toxicities in early-stage NSCLC patients who were &gt;80 years old.
Aorto-esophageal fistula: successful open surgical management of two cases
Authors: Pahwa Siddharth , Bhattacharya Susmit , Mukhopadhyay Siddhartha , Verma Ashok 
Published: April 2020

Abstract
An aorto-esophageal fistula (AEF) is a rare yet life-threatening cause of upper gastrointestinal bleeding. We report our experience with open surgical management of two cases of AEF. Both cases presented with almost identical presentations: hematemesis and hemodynamic instability. The aorta in the first patient was normal; the defect was small and was repaired with a Dacron patch. The second patient had an aneurysmal aorta, which was replaced with a Dacron graft. Both cases were performed under partial bypass. The esophageal rent in both patients was debrided, primarily closed and buttressed with a vascularized intercostal pedicle. Nonavailability of endovascular personnel and equipment along with hemodynamic instability of the patient influenced our surgical strategy. Long-term follow-up of these patients is necessary to analyze the outcomes of our surgical repair.
Into another dimension: how streptophyte algae gained morphological complexity
Authors: Buschmann Henrik 
Published: April 2020

AbstractLand plants with elaborated three-dimensional (3D) body plans have evolved from streptophyte algae. The streptophyte algae are known to exhibit varying degrees of morphological complexity, ranging from single-celled flagellates to branched macrophytic forms exhibiting tissue-like organization. In this review, I discuss mechanisms by which, during evolution, filamentous algae may have gained 2D and eventually 3D body plans. There are, in principle, two mechanisms by which an additional dimension may be added to an existing algal filament or cell layer: first, by tip growth-mediated branching. An example of this mechanism is the emergence and polar expansion of root hairs from land plants. The second possibility is the rotation of the cell division plane. In this case, the plane of the forthcoming cell division is rotated within the parental cell wall. This type of mechanism corresponds to the formative cell division seen in meristems of land plants. This literature review shows that of the extant streptophyte algae, the Charophyceae and Coleochaetophyceae are capable of performing both mechanisms, while the Zygnematophyceae (the actual sister to land plants) show tip growth-based branching only. I finally discuss how apical cells with two or three cutting faces, as found in mosses, may have evolved from algal ancestors.
Mutagenesis mechanism of the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine
Authors: Koag Myong-Chul , Jung Hunmin , Lee Seongmin 
Published: April 2020

Abstract
Reactive oxygen species generate the genotoxic 8-oxoguanine (oxoG) and 8-oxoadenine (oxoA) as major oxidative lesions. The mutagenicity of oxoG is attributed to the lesion's ability to evade the geometric discrimination of DNA polymerases by adopting Hoogsteen base pairing with adenine in a Watson–Crick-like geometry. Compared with oxoG, the mutagenesis mechanism of oxoA, which preferentially induces A-to-C mutations, is poorly understood. In the absence of protein contacts, oxoA:G forms a wobble conformation, the formation of which is suppressed in the catalytic site of most DNA polymerases. Interestingly, human DNA polymerase η (polη) proficiently incorporates dGTP opposite oxoA, suggesting the nascent oxoA:dGTP overcomes the geometric discrimination of polη. To gain insights into oxoA-mediated mutagenesis, we determined crystal structures of polη bypassing oxoA. When paired with dGTP, oxoA adopted a syn-conformation and formed Hoogsteen pairing while in a wobble geometry, which was stabilized by Gln38-mediated minor groove contacts to oxoA:dGTP. Gln38Ala mutation reduced misinsertion efficiency ∼55-fold, indicating oxoA:dGTP misincorporation was promoted by minor groove interactions. Also, the efficiency of oxoA:dGTP insertion by the X-family polβ decreased ∼380-fold when Asn279-mediated minor groove contact to dGTP was abolished. Overall, these results suggest that, unlike oxoG, oxoA-mediated mutagenesis is greatly induced by minor groove interactions.
A distinct role for recombination repair factors in an early cellular response to transcription–replication conflicts
Authors: Shao Xin , Joergensen Amalie M , Howlett Niall G , Lisby Michael , Oestergaard Vibe H 
Published: April 2020

Abstract
Transcription–replication (T–R) conflicts are profound threats to genome integrity. However, whilst much is known about the existence of T–R conflicts, our understanding of the genetic and temporal nature of how cells respond to them is poorly established. Here, we address this by characterizing the early cellular response to transient T–R conflicts (TRe). This response specifically requires the DNA recombination repair proteins BLM and BRCA2 as well as a non-canonical monoubiquitylation-independent function of FANCD2. A hallmark of the TRe response is the rapid co-localization of these three DNA repair factors at sites of T–R collisions. We find that the TRe response relies on basal activity of the ATR kinase, yet it does not lead to hyperactivation of this key checkpoint protein. Furthermore, specific abrogation of the TRe response leads to DNA damage in mitosis, and promotes chromosome instability and cell death. Collectively our findings identify a new role for these well-established tumor suppressor proteins at an early stage of the cellular response to conflicts between DNA transcription and replication.
Exceptional band touching for strongly correlated systems in equilibrium
Authors: Yoshida Tsuneya , Peters Robert , Kawakami Norio , Hatsugai Yasuhiro 
Published: April 2020

Abstract
Quasi-particles described by Green‘s functions of equilibrium systems exhibit non-Hermitian topological phenomena because of their finite lifetime. This non-Hermitian perspective on equilibrium systems provides new insights into correlated systems and attracts much interest because of its potential to solve open questions in correlated compounds. We provide a concise review of the non-Hermitian topological band structures for quantum many-body systems in equilibrium, as well as their classification.
Mineralized collagen scaffolds fabricated with amniotic membrane matrix increase osteogenesis under inflammatory conditions
Authors: Dewey Marley J , Johnson Eileen M , Slater Simona T , Milner Derek J , Wheeler Matthew B , Harley Brendan A C 
Published: April 2020

AbstractDefects in craniofacial bones occur congenitally, after high-energy impacts, and during the course of treatment for stroke and cancer. These injuries are difficult to heal due to the overwhelming size of the injury area and the inflammatory environment surrounding the injury. Significant inflammatory response after injury may greatly inhibit regenerative healing. We have developed mineralized collagen scaffolds that can induce osteogenic differentiation and matrix biosynthesis in the absence of osteogenic media or supplemental proteins. The amniotic membrane is derived from placentas and has been recently investigated as an extracellular matrix to prevent chronic inflammation. Herein, we hypothesized that a mineralized collagen–amnion composite scaffold could increase osteogenic activity in the presence of inflammatory cytokines. We report mechanical properties of a mineralized collagen–amnion scaffold and investigated osteogenic differentiation and mineral deposition of porcine adipose-derived stem cells within these scaffolds as a function of inflammatory challenge. Incorporation of amniotic membrane matrix promotes osteogenesis similarly to un-modified mineralized collagen scaffolds, and increases in mineralized collagen–amnion scaffolds under inflammatory challenge. Together, these findings suggest that a mineralized collagen–amnion scaffold may provide a beneficial environment to aid craniomaxillofacial bone repair, especially in the course of defects presenting significant inflammatory complications.
Arthritis autoantibodies in individuals without rheumatoid arthritis: follow-up data from a Dutch population-based cohort (Lifelines)
Authors: Westra Johanna , Brouwer Elisabeth , Raveling-Eelsing Elisabeth , Arends Suzanne , Eman Abdulle Amaal , Roozendaal Caroline , van Delft Myrthe A M , Toes Rene E M , Trouw Leendert A , Vissink Arjan , de Smit Menke J 
Published: April 2020

Abstract

Objectives
To assess whether the presence of arthritis autoantibodies alongside IgG ACPA predicts clinically suspect arthralgia in ACPA-positive subjects without RA.

Methods
In the population-based Lifelines cohort (n = 40 136), 308 IgG ACPA-positive individuals without RA were present. Serum levels of IgA ACPA, IgA and IgM RF, and IgG anti-carbamylated antibodies were measured at baseline. Individuals were divided based on the Connective tissue disease Screening Questionnaire after 2 years follow-up. Antibodies to Porphyromonas gingivalis were determined at baseline and related to presence of periodontitis and joint complaints at 2 years follow-up.

Results
Of 308 subjects 53.6% were also seropositive for IgA ACPA, 42.2% for IgM RF, 23.7% for IgA RF and 13.6% for anti-carbamylated antibodies. We defined 75 persons with clinically suspect arthralgia at risk for RA based on CTD Screening Questionnaire at follow-up. Significantly more seropositivity for IgM RF and higher levels of IgG ACPA, IgA ACPA and IgM RF were found in clinically suspect arthralgia compared with no-clinically suspect arthralgia. In multivariate logistic regression correcting for age, gender and never smoking, positivity for three or more extra autoantibodies was significantly associated with clinically suspect arthralgia. Although levels of anti-P. gingivalis were not different between groups, they were significantly correlated to levels of both RFs, and both ACPAs in clinically suspect arthralgia.

Conclusions
ACPA-positive individuals without RA who develop clinically suspect arthralgia have more and higher levels of other arthritis autoantibodies at baseline. Levels of anti-P. gingivalis are not related to self-reported periodontitis or clinically suspect arthralgia, but are correlated to arthritis autoantibodies in clinically suspect arthralgia.
Correlation of serostatus and viraemia levels among Indian dengue patients at the time of first diagnosis
Authors: Kulkarni Ruta , Shrivastava Shubham , Patil Harshad P , Tiraki Divya , Mishra Akhilesh Chandra , Arankalle Vidya A 
Published: April 2020

AbstractBackgroundDengue is a public health problem worldwide. Therapeutic monoclonal antibodies (MAbs) against dengue virus (DENV) are likely to be available soon. In view of the feasibility issues pertaining to pretreatment viraemia quantitation for therapy decisions, we conducted this study for investigation of a correlation between patient serostatus (NS1/immunoglobulin M [IgM]/IgG) and viraemia levels among Indian dengue patients at the time of first diagnosis.MethodsThe study included 297 serum samples from dengue patients in Pune, India. The samples were tested for NS1, IgM and IgG (capture enzyme-linked immunosorbent assay [ELISA] for identifying secondary dengue) using Panbio ELISAs. Quantitation of viraemia was conducted using an NS1 ELISA-based 50% tissue culture infectious dose (TCID50) test in Vero cells.ResultsViraemia was detectable only among NS1-positive patients (n = 229, range 0.5–8.3 logTCID50/ml) with a mean titre of 1.9 logTCID50/ml. Among the NS1-positive patients, DENV titres were higher in IgM-negative than IgM-positive patients (p &lt; 0.0001) and in primary (IgG &lt; 18 Panbio units) versus secondary (IgG &gt; 22 Panbio units) dengue patients (p = 0.002). Virus titres were higher during the first 3 days of illness and decreased later (p = 0.005).ConclusionsThe study provides a range of infectious DENV titres in relation to serologic status among dengue patients in India. The data suggest the possibility of using serological markers (NS1/IgM) as a basis for treatment decisions.
Predictors of Mother's Own Milk Feeding at Discharge in Preterm Infants
Authors: Lussier Mary M. , Tosi Lauren , Brownell Elizabeth A. 
Published: April 2020

Background:The dose–response benefits of human milk for preterm infants are well documented. Understanding factors that influence duration of mother's own milk (MOM) receipt may have important clinical implications.Purpose:To identify variables that significantly affect whether or not preterm infants receive their own mother's milk at discharge.Methods:Maternal–infant dyads were eligible for inclusion if the infant was born between August 1, 2010, and July 31, 2015, was born at 32 weeks' gestation or less, or was 1800 g or less (institutional donor milk receipt criteria). Bivariate and multivariable regression analyses were performed.Results:Of 428 observations, 258 (60.3%) received MOM at discharge and 170 (39.7%) did not. Maternal characteristics that were protective for MOM receipt at discharge were non-Hispanic race, married, partner support, more educated, and private insurance. Protective infant characteristics were higher gestational age at birth, higher percentage of MOM feedings, fewer ventilator days, and more days of direct lactation. In multivariable logistic regression, the odds of receiving MOM at discharge significantly (odds ratio = 1.93; 95% confidence interval, 1.72-2.16;P< .001) increased with the increasing proportion of MOM. Regression analysis showed that gestational age and increased maternal age increased the likelihood of MOM receipt at discharge.Implications for Practice:Clinicians will understand the significant effects even small increases in milk volume have on duration of MOM receipt at discharge, informing them of the importance of strategies to encourage and improve milk expression.Implications for Research:Future research studying critical time periods when mothers are most likely to reduce milk expression may have significant clinical importance.