bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolomic profiling identifies novel associations with Electrolyte and Acid-Base Homeostatic patterns
Authors: Menni Cristina , McCallum Linsay , Pietzner Maik , Zierer Jonas , Aman Alisha , Suhre Karsten , Mohney Robert P. , Mangino Massimo , Friedrich Nele , Spector Tim D. , Padmanabhan Sandosh 
Published: October 2019

AbstractElectrolytes have a crucial role in maintaining health and their serum levels are homeostatically maintained within a narrow range by multiple pathways involving the kidneys. Here we use metabolomics profiling (592 fasting serum metabolites) to identify molecular markers and pathways associated with serum electrolyte levels in two independent population-based cohorts. We included 1523 adults from TwinsUK not on blood pressure-lowering therapy and without renal impairment to look for metabolites associated with chloride, sodium, potassium and bicarbonate by running linear mixed models adjusting for covariates and multiple comparisons. For each electrolyte, we further performed pathway enrichment analysis (PAGE algorithm). Results were replicated in an independent cohort. Chloride, potassium, bicarbonate and sodium associated with 10, 58, 36 and 17 metabolites respectively (each P < 2.1 × 10−5), mainly lipids. Of all the electrolytes, serum potassium showed the most significant associations with individual fatty acid metabolites and specific enrichment of fatty acid pathways. In contrast, serum sodium and bicarbonate showed associations predominantly with amino-acid related species. In the first study to examine systematically associations between serum electrolytes and small circulating molecules, we identified novel metabolites and metabolic pathways associated with serum electrolyte levels. The role of these metabolic pathways on electrolyte homeostasis merits further studies.
Subtle Cr isotope signals track the variably anoxic Cryogenian interglacial period with voluminous manganese accumulation and decrease in biodiversity
Authors: Xu Lingang , Frank Anja B. , Lehmann Bernd , Zhu Jianming , Mao Jingwen , Ju Yongze , Frei Robert 
Published: October 2019

AbstractEarth’s atmosphere experienced a step of profound oxygenation during the Neoproterozoic era, accompanied by diversification of animals. However, during the Cryogenian period (720–635 million years ago) Earth experienced its most severe glaciations which likely impacted marine ecosystems and multicellular life in the oceans. In particular, large volumes of Mn and Fe accumulated during the interglacial intervals of the Cryogenian glaciations, indicating large anoxic marine metal reservoirs. Here we present chromium isotope-, rare earth element-, and redox-sensitive trace element data of sedimentary rocks from the interglacial Datangpo Formation deposited between the Sturtian and Marinoan glaciations in South China, in an attempt to investigate the oxidation state of the oceans and atmosphere. Both the Cr isotope and trace element data indicate mainly anoxic water conditions with cryptic oxic surface water incursions after the Sturtian glaciation. Glacial-fed manganese precipitated as manganese carbonate in anoxic basins, and the non-fractionated δ53Cr record of −0.10 ± 0.06‰ identifies anoxic conditions with a cryptic component of slightly fractionated Cr isotope composition in manganese ore, in line with distinctly fractionated Mo isotope composition. Both the manganese carbonate ore and the black shales exhibit very low redox-sensitive element concentrations. Our study demonstrates that the oxygenation of the seawater, and inferably of the atmosphere, at the beginning of the Cryogenian interglacial interval was much subdued. The post-glacial rebound then allowed the Ediacaran biological diversity.
Ultrasound-derived changes in thickness of human ankle plantar flexor muscles during walking and running are not homogeneous along the muscle mid-belly region
Authors: Hodson-Tole E. F. , Lai A. K. M. 
Published: October 2019

AbstractSkeletal muscle thickness is a valuable indicator of several aspects of a muscle’s functional capabilities. We used computational analysis of ultrasound images, recorded from 10 humans walking and running at a range of speeds (0.7–5.0 m s−1), to quantify interactions in thickness change between three ankle plantar flexor muscles (soleus, medial and lateral gastrocnemius) and quantify thickness changes at multiple muscle sites within each image. Statistical analysis of thickness change as a function of stride cycle (1d statistical parametric mapping) revealed significant differences between soleus and both gastrocnemii across the whole stride cycle as they bulged within the shared anatomical space. Within each muscle, changes in thickness differed between measurement sites but not locomotor condition. For some of the stride, thickness measures taken from the distal-mid image region represented the mean muscle thickness, which may therefore be a reliable region for these measures. Assumptions that muscle thickness is constant during a task, often made in musculoskeletal models, do not hold for the muscles and locomotor conditions studied here and researchers should not assume that a single thickness measure, from one point of the stride cycle or a static image, represents muscle thickness during dynamic movements.
Identification of Molecular Fluorophore as a Component of Carbon Dots able to Induce Gelation in a Fluorescent Multivalent-Metal-Ion-Free Alginate Hydrogel
Authors: Kasak Peter , Danko Martin , Zavahir Sifani , Mrlik Miroslav , Xiong Yuan , Yousaf Ammar Bin , Lai Wing-Fu , Krupa Igor , Tkac Jan , Rogach Andrey L. 
Published: October 2019

AbstractWe introduce a simple approach to fabricate fluorescent multivalent metal ion-free alginate hydrogels, which can be produced using carbon dots accessible from natural sources (citric acid and L-cysteine). Molecular fluorophore 5-oxo-2,3-dihydro-5H-[1,3]-thiazolo[3,2-a] pyridine-3,7-dicarboxylic acid (TPDCA), which is formed during the synthesis of carbon dots, is identified as a key segment for the crosslinking of hydrogels. The crosslinking happens through dynamic complexation of carboxylic acid groups of TPDCA and alginate cages along with sodium ions. The TPDCA derived hydrogels are investigated regarding to their thermal, rheological and optical properties, and found to exhibit characteristic fluorescence of this aggregated molecular fluorophore. Moreover, gradient hydrogels with tunable mechanical and optical properties and controlled release are obtained upon immersion of the hydrogel reactors in solutions of divalent metal ions (Ca2+, Cu2+, and Ni2+) with a higher affinity to alginate.
Underlying structure in the dynamics of chase and escape interactions
Authors: Tsutsui Kazushi , Shinya Masahiro , Kudo Kazutoshi 
Published: October 2019

AbstractChase and escape behaviors are important skills in many sports. Previous studies have described the behaviors of the attacker (escaper) and defender (chaser) by focusing on their positional relationship and have presented several key parameters that affect the outcome (successful attack or defense). However, it remains unclear how each individual agent moves, and how the outcome is determined in this type of interaction. To address these questions, we constructed a chase and escape task in a virtual space that allowed us to manipulate agents’ kinematic parameters. We identified the basic strategies of each agent and their robustness to changes in their parameters. Moreover, we identified the determinants of the outcome and a geometrical explanation of their importance. Our results revealed the underlying structure of a simplified human chase and escape interaction and provided the insight that, although each agent apparently moves freely, their strategies in two-agent interactions are in fact rather constrained.
A PQ-loop protein Ypq2 is involved in the exchange of arginine and histidine across the vacuolar membrane of Saccharomyces cerevisiae
Authors: Kawano-Kawada Miyuki , Manabe Kunio , Ichimura Haruka , Kimura Takumi , Harada Yuki , Ikeda Koichi , Tanaka Shiho , Kakinuma Yoshimi , Sekito Takayuki 
Published: October 2019

AbstractIn nutrient-rich conditions, basic amino acids are actively accumulated into the vacuoles by H+-coupled transporters in Saccharomyces cerevisiae. In addition to the H+-coupled systems, the existence of an exchanger for arginine and histidine was indicated by kinetic analysis using isolated vacuolar membrane vesicles; however, the gene(s) involved in the activity has not been identified. Here, we show that the uptake activity of arginine driven by an artificially imposed histidine gradient decreased significantly by the disruption of the gene encoding vacuolar PQ-loop protein Ypq2, but not by those of Ypq1 and Ypq3. The exchange activity was restored by the expression of YPQ2. Furthermore, the substitution of a conserved proline residue, Pro29, in Ypq2 greatly decreased the exchange activity. These results suggest that Ypq2 is responsible for the exchange activity of arginine and histidine across the vacuolar membrane, and the conserved proline residue in the PQ-loop motif is required for the activity.
Seasonal distribution and population dynamics of limnic microalgae and their association with physico-chemical parameters of river Noyyal through multivariate statistical analysis
Authors: Kumar Pandian Suresh , Thomas Jibu 
Published: October 2019

AbstractThe present investigation embarks on understanding the relationship between microalgal species assemblages and their associated physico-chemical parameter dynamics of the catchment region of river Noyyal. Totally, 142 microalgae cultures belonging to 10 different families were isolated from five different sites during four seasons and relative percentage distribution showed that Scenedesmaceae (36.6%) and site S1 (26.4%) with predominant microalgae population. Diversity indices revealed that microalgae communities were characterized by high Hʹ index, lower Simpson dominance, and Margalef index value with indefinite patterns of annual variations. Results showed that variation in the physico-chemical parameters in each sampling site has its impact on the microalgae population during each season. Multivariate statistical analysis viz., Karl Pearson’s correlation coefficient, principal component analysis, and canonical correspondence analysis were applied on microalgae species data, to evaluate the seasonal relationship between microalgae and physico-chemical parameters. The findings of our study concluded that the physico- chemical parameters influenced the dominant taxa of microalgae Chlorellaceae, Scenedesmaceae and Chlorococcaceae in river Noyyal and gives a base data for the seasonal and dynamic relationship between environmental parameters and microalgae population.
Highly Tough, Biocompatible, and Magneto-Responsive Fe3O4/Laponite/PDMAAm Nanocomposite Hydrogels
Authors: Lee Jin Hyun , Han Wen Jiao , Jang Hyo Seon , Choi Hyoung Jin 
Published: October 2019

AbstractMagneto-responsive hydrogels (MRHs) have attracted considerable attention in various applications owing to their smart response to an externally applied magnetic field. However, their practical uses in biomedical fields are limited by their weak mechanical properties and possible toxicity to the human body. In this study, tough, biocompatible, and magneto-responsive nanocomposite hydrogels (MR_NCHs) were developed by the in-situ free-radical polymerization of N, N-dimethylacrylamide (DMAAm) and laponite and Fe3O4 nanoparticles. The effects of the concentrations of DMAAm, water, and laponite and Fe3O4 nanoparticles in the pre-gel solutions or mixtures on the viscoelastic and mechanical properties of the corresponding hydrogels were examined by performing rheological and tensile tests, through which the mixture composition producing the best MR_NCH system was optimized. The effects were also explained by the possible network structures of the MR_NCHs. Moreover, the morphology, chemical structure, and thermal and mechanical properties of the MR_NCHs were analyzed, while comparing with those of the poly(DMAAm) (PDMAAm) hydrogels and laponite/PDMAAm NCHs. The obtained optimal MR_NCH exhibited noticeable magnetorheological (MR) behavior, excellent mechanical properties, and good biocompatibility. This study demonstrates how to optimize the best Fe3O4/laponite/PDMAAm MR_NCH system and its potential as a soft actuator for the pharmaceutical and biomedical applications.
Changes in the consumption of antiepileptics and psychotropic medicines after starting low dose naltrexone: A nation-wide register-based controlled before-after study
Authors: Raknes Guttorm , Småbrekke Lars 
Published: October 2019

AbstractIn this controlled before-after study based on data from the Norwegian Prescription Database, we examine whether starting off-label use of Low Dose Naltrexone (LDN) is followed by changes in the consumption of psychotropic medicines including antiepileptics. Patients that collected LDN for the first time in 2013 (N = 11247) were included and stratified into three groups based on LDN exposure. We compared differences in means of cumulative number of defined daily doses (DDD) as well as changes in the number of users one year before and one year after starting LDN. There was a dose-response association between increasing LDN exposure and reductions in the number of users of antiepileptics, antipsychotics and antidepressants. There were significant difference-in-differences in DDDs between the groups with the lowest and highest LDN exposure of antipsychotics (1.4 DDD, 95% CI 0.4 to 2.3, p = 0.007), and in number of users of antiepileptics (3.1% points, 95% CI 1.6% to 4.6%, p < 0.001), antipsychotics (2.1% points, 95% CI 1.2% to 3%, p < 0.001), and antidepressants (2.8% points, 95% CI 1.1% to 4.4%, p = 0.001). The findings show an association between the initiation of persistent LDN use and reduced consumption of several psychotropic medicines and antiepileptics. Beneficial effects of LDN in the treatment of psychiatric diseases cannot be ruled out.
EV-associated miRNAs from pleural lavage as potential diagnostic biomarkers in lung cancer
Authors: Roman-Canal Berta , Moiola Cristian Pablo , Gatius Sònia , Bonnin Sarah , Ruiz-Miró Maria , González Esperanza , Ojanguren Amaia , Recuero José Luis , Gil-Moreno Antonio , Falcón-Pérez Juan M. , Ponomarenko Julia , Porcel José M. , Matias-Guiu Xavier , Colas Eva 
Published: October 2019

AbstractLung cancer is the leading cause of cancer-related deaths among men and women in the world, accounting for the 25% of cancer mortality. Early diagnosis is an unmet clinical issue. In this work, we focused to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the pleural lavage, a proximal fluid in lung cancer patients, as a source of potential biomarkers. We isolated EVs by ultracentrifuge method from 25 control pleural fluids and 21 pleural lavages from lung cancer patients. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 288 out of the 754 miRNAs that were contained in the OpenArray. The differential expression analysis yielded a list of 14 miRNAs that were significantly dysregulated (adj. p-value < 0.05 and logFC lower or higher than 3). Using Machine Learning approach we discovered the lung cancer diagnostic biomarkers; miRNA-1-3p, miRNA-144-5p and miRNA-150-5p were found to be the best by accuracy. Accordance with our finding, these miRNAs have been related to cancer processes in previous studies. This results opens the avenue to the use of EV-associated miRNA of pleural fluids and lavages as an untapped source of biomarkers, and specifically, identifies miRNA-1-3p, miRNA-144-5p and miRNA 150-5p as promising biomarkers of lung cancer diagnosis.
Habitat availability explains variation in climate-driven range shifts across multiple taxonomic groups
Authors: Platts Philip J. , Mason Suzanna C. , Palmer Georgina , Hill Jane K. , Oliver Tom H. , Powney Gary D. , Fox Richard , Thomas Chris D. 
Published: October 2019

AbstractRange shifting is vital for species persistence, but there is little consensus on why individual species vary so greatly in the rates at which their ranges have shifted in response to recent climate warming. Here, using 40 years of distribution data for 291 species from 13 invertebrate taxa in Britain, we show that interactions between habitat availability and exposure to climate change at the range margins explain up to half of the variation in rates of range shift. Habitat generalists expanded faster than more specialised species, but this intrinsic trait explains less of the variation in range shifts than habitat availability, which additionally depends on extrinsic factors that may be rare or widespread at the range margin. Similarly, while climate change likely underlies polewards expansions, we find that more of the between-species variation is explained by differences in habitat availability than by changes in climatic suitability. A model that includes both habitat and climate, and their statistical interaction, explains the most variation in range shifts. We conclude that climate-change vulnerability assessments should focus as much on future habitat availability as on climate sensitivity and exposure, with the expectation that habitat restoration and protection will substantially improve species’ abilities to respond to uncertain future climates.
Spatiotemporal dynamics of different CO2 fixation strategies used by prokaryotes in a dimictic lake
Authors: Alfreider Albin , Tartarotti Barbara 
Published: October 2019

AbstractThe Calvin-Benson-Bassham (CBB) cycle and the 3-hydroxypropionate/4-hydroxybutyrate (HP/HB) cycle are two inorganic carbon assimilation pathways widely used by prokaryotic autotrophs in lakes. We investigated the effect of mixing periods and stable water stratification patterns on the trajectories of both CO2 fixation strategies in a dimictic lake (Piburger See), because information on the spatiotemporal dynamics of prokaryotes using these pathways in freshwater ecosystems is far from complete. Based on a quantitative approach (droplet digital PCR) of genes coding for key enzymes in different CO2 assimilation pathways, nine depths covering the entire water column were investigated on a monthly basis for one year. Our data show that the abundance of photoautotrophs and obligate chemolithoautotrophs preferentially using form IA RubisCO was determined by seasonal variations. Highest numbers were observed in summer, while a strong decline of prokrayotes using RubisCO form IA was measured between December and May, the period where the lake was mostly covered by ice. The spatiotemporal distribution patterns of genes coding for RubisCO form IC genes, an enzyme usually used by facultative autotrophs for CO2 assimilation, were less pronounced. Bacteria harboring RubisCO form II were dominating the oxygen limited hypolimnion, while nitrifying Thaumarchaeota using the HP/HB cycle were of minor importance in the lake. Our data reveal that the seasonal heterogeneity, which is determined by the dimictic thermal regime of the lake, results in pronounced spatiotemporal changes of different CO2 assimilation pathways with depth-dependent environmental parameters as key factors for their distribution.
Characterizing ecosystem phenological diversity and its macroecology with snow cover phenology
Authors: Lin Yi , Hyyppä Juha 
Published: October 2019

AbstractOne critical challenge of exploring flora phenology is on characterizing ecosystem phenological diversity (EPD), and thus how EPD’s performance is influenced by climate changes has also been an open macro-ecological question. To fill these two gaps, we proposed an innovative method for reflecting EPD, by taking the advantage of the often-classified inverse factor of spatial resolution discrepancy between the used remote sensing datasets of vegetation phenological dates (green-up and brown-up) and snow cover phenological dates (SPDs) (onset and end) around the Arctic, and further, we examined the cross response/feedbacks of the two kinds of EPDs to the two categories of SPDs. We found that the circumpolar green-up and brown-up EPDs both were shrinking, driven more by the delaying of the onset SPDs than the advancing of the end SPDs; North America and North Eurasia performed with inconsistent EPD response/feedbacks to the related SPD anomalies; and further, the EPD-SPD response/feedbacks in some locations exhibited the time-lag effect, e.g., the green-up EPDs made the strongest response to the onset SPDs of two years earlier. Overall, the validated method and the new findings are of implications for improving the phenology modules in Earth system models, and the contributions of the present study have enlightening significance for kicking off the new EPD branch in macrosystem phenological ecology.
Procyanidin A1 Alleviates Inflammatory Response induced by LPS through NF-κB, MAPK, and Nrf2/HO-1 Pathways in RAW264.7 cells
Authors: Han Shan , Gao Hongwei , Chen Shaoru , Wang Qinqin , Li Xinxing , Du Li-Jun , Li Jun , Luo Ying-Ying , Li Jun-Xiu , Zhao Li-Chun , Feng Jianfang , Yang Shilin 
Published: October 2019

AbstractInflammation is a complex physiological process that poses a serious threat to people’s health. However, the potential molecular mechanisms of inflammation are still not clear. Moreover, there is lack of effective anti-inflammatory drugs that meet the clinical requirement. Procyanidin A1 (PCA1) is a monomer component isolated from Procyanidin and shows various pharmacological activities. This study further demonstrated the regulatory role of PCA1 on lipopolysaccharide (LPS)-stimulated inflammatory response and oxidative stress in RAW264.7 cells. Our data showed that PCA1 dramatically attenuated the production of pro-inflammatory cytokines such as NO, iNOS, IL-6, and TNF-α in RAW264.7 cells administrated with LPS. PCA1 blocked IκB-α degradation, inhibited IKKα/β and IκBα phosphorylation, and suppressed nuclear translocation of p65 in RAW264.7 cells induced by LPS. PCA1 also suppressed the phosphorylation of JNK1/2, p38, and ERK1/2 in LPS-stimulated RAW264.7 cells. In addition, PCA1 increased the expression of HO-1, reduced the expression of Keap1, and promoted Nrf2 into the nuclear in LPS-stimulated RAW264.7 cells. Cellular thermal shift assay indicated that PCA1 bond to TLR4. Meanwhile, PCA1 inhibited the production of intracellular ROS and alleviated the depletion of mitochondrial membrane potential in vitro. Collectively, our data indicated that PCA1 exhibited a significant anti-inflammatory effect, suggesting that it is a potential agent for the treatment of inflammatory diseases.