bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estudo do efeito do tratamento térmico nos compostos bioativos em PANC
Authors: Benevides Clícia Maria de Jesus , Costa Cirlene Cardoso Monteiro , Cardoso Yago Pinto , Lopes Mariangela Vieira , Montes Simone de Souza , Souza Antonio Carlos dos Santos 
Published: November 2020

Este trabalho objetivou avaliar o efeito do tratamento térmico (TT) nos compostos bioativos e antinutricionais em diferentes PANC (Beldroega, Bredo, Chaya, Cana de macaco, Hortelã com borda, Língua de vaca, Serralha e a Urtiga mansa). As concentrações dos compostos bioativos - fenólicos totais (FT) e flavonoides; dos antinutricionais (taninos e oxalatos) e a atividade antioxidante (AA) foram realizadas segundo as Normas Analíticas do Instituto Adolfo Lutz. Os teores (mg/100g) dos compostos nas PANC antes do TT variaram da seguinte forma: compostos bioativos - FT de 2,04±0,04 a 37,40±0,46, os flavonoides de 145,96±0,61 a 410,73±1,93; os compostos antinutricionais - taninos de 4,47±0,46 a 211,74±2,96, e oxalatos de 2,61±0,13 a 4,41±0,22; a AA (IC50) variou de 2,13±0,05 a 7,05±1,36. O TT aplicado nas amostras proporcionou aumento no percentual da concentração dos compostos na maioria das PANC que variou de: 38,13 a 99,84% (FT); 1,2 a 37% (flavonoides); 21,21 a 116,55% (taninos); 45,92 a 77,63 (oxalatos), assim como na AA, que variou de 14,5 a 67%. Por outro lado, houve uma variação no percentual da redução nas outras PANC de: 45 a 73% (FT); 8 a 29% (flavonoides); 15 a 74 % (taninos); 5,36 a 27,44% (oxalatos) e de 44,49 a 79,84% (AA). Conclui-se que, de maneira geral, o TT aplicado favoreceu o aumento da concentração dos compostos bioativos e da AA nas PANC, o mesmo não ocorrendo para os fatores antinutricionais.
Pesquisa básica e pesquisa aplicada: uma análise a partir da produção científica sobre COVID-19
Authors: Zucatto Luis Carlos , Freitas Rodrigo Uszacki Carvalho de , Marzzoni David Nogueira Silva 
Published: November 2020

Este estudo teve como objetivo realizar um levantamento acerca de publicações acadêmicas que abordam o tema COVID-19, em vista de identificar a qual tipo de pesquisa – básica ou aplicada, estariam alinhadas essas publicações. Como base de dados, escolheu-se o Google Acadêmico e utilizou-se como descritor COVID-19, que deveria estar no título ou no abstract. A busca foi realizada em 03 de abril de 2020, identificando-se um total de 1.290.000 resultados, com o filtro Em Qualquer Idioma. Desse universo, delimitou-se a amostra com os parâmetros de 5% de margem de erro e 90% de intervalo de confiança, o que resultou em 273 trabalhos, ranqueados por relevância. Para a classificação em pesquisa básica ou pesquisa aplicada utilizaram-se pressupostos teóricos de Bush (1945), da Orgazination for Economic Cooperation and Development (OECD,1994), de Stokes (2005) e de Riazuddin (2007). Os resultados evidenciam a prevalência de produções acadêmicas classificadas como pesquisa básica (68,46%) e a China como o País do qual mais se publicaram estudos acerca dessa temática (41,96%).
Atividade assistida por animais: uma estratégia para pacientes com transtornos mentais
Authors: Sapin Carolina da Fonseca , Lima Camila Moura de , Costa Juliana Costa da , Costa Monike , Almeida Débora Matilde de , Mechereffe Beatriz Maksud , Nobre Márcia de Oliveira 
Published: November 2020

As atividades assistidas por animais (AAA) consistem em atividades voltadas para recreação, entretenimento e distração através do contato com animais. Esse vínculo pode trazer diversos benefícios aos assistidos quanto aos aspectos emocionais e fisiológicos. O objetivo deste trabalho é avaliar a resposta dos pacientes com doenças mentais em relação às AAA. Foram realizadas duas sessões de AAA em uma instituição para doentes mentais na cidade de Pelotas, em uma sala de recreação. Previa e posteriormente ao contato com os cães eram aferidas a frequência cardíaca e as pressões arteriais dos assistidos. Ainda foi aplicada a escala de faces de Andrews em ambos os momentos. As sessões tiveram duração média de uma hora e participação de 11 pacientes. Alguns pacientes tinham preferência em realizar atividades com contato direto com o cão, assim como outros preferiam atividades lúdicas com a temática dos cães. Os cães agiram com motivadores e proporcionaram que os assistidos permanecessem maior tempo nas atividades, dessa forma, promovendo descontração e relaxamento. As pressões arteriais e frequência cardíaca em grande parte dos assistidos se mantiveram ou reduziram. As atividades colaboraram para que os pacientes se tornassem mais comunicativos, demonstrassem afeto pelos cães e pela equipe envolvida. As escala de faces de Andrews indicaram que todos mantiveram a percepção inicial ou a melhoraram. Conclui-se que as AAA proporcionaram benefícios de bem-estar aos pacientes com doenças mentais quanto à manutenção da pressão arterial e frequência cardíaca, melhora na socialização, aspectos comportamentais e na autopercepção da felicidade.
Indicadores sociais e econômicos como instrumento no processo de tomada de decisão de um Instituto Federal
Authors: Nunes Valnei , Ribeiro Eduardo Augusto Werneck 
Published: November 2020

Este artigo é resultado da pesquisa vinculada ao Programa de Pós-graduação em Educação Profissional e Tecnológica, Programa de Mestrado do Instituto Federal, polo campus de Blumenau. Tem como temática os indicadores sociais e econômicos, como instrumento no processo (re) conhecimento do contexto de atuação de um campus do Instituto Federal. Com base nas reflexões realizadas nesta pesquisa em relação às proximidades e distanciamentos de um campus, no que se refere ao seu compromisso legal, busca-se responder em que medida os indicadores sociais e econômicos contribuem para a gestão na tomada de decisão. A partir das finalidades dos Institutos Federais, segundo a Lei 11.892/2008, compreende-se que um campus assume o compromisso de contribuir com sua área de abrangência. Questão esta que reclama, entre outros aspectos, no (re) conhecimento constante dos Arranjos Produtivos, Sociais Culturais Locais, sendo o mapeamento das potencialidades socioeconômicas e culturais da realidade, fator indispensável a ser observado, seja no percurso de implantação e implementação de um campus do Instituto Federal, quanto no processo de (re) avaliação contínua de suas ofertas. Quanto à abordagem, a pesquisa caracterizou-se por qualitativa e quantitativa; de natureza aplicada; quanto aos seus objetivos, exploratória e descritiva; bibliográfico/documental e pesquisa de campo em relação aos seus procedimentos. Sobre os resultados obtidos, destaca-se a lacuna existente na definição sobre a área de abrangência de um campus, a construção de um banco de dados com indicadores desagregados por municípios e sua série histórica.
Foundation and Clinical Evaluation of a New Method for Detecting SARS-CoV-2 Antigen by Fluorescent Microsphere Immunochromatography
Authors: Zhang Chunyan , Zhou Lei , Du Kang , Zhang Ying , Wang Jing , Chen Lijuan , Lyu Yanning , Li Jun , Liu Hao , Huo Junli , Li Fei , Wang Jiayi , Sang Peipei , Lin Si , Xiao Yi , Zhang Kan , He Kunlun 
Published: November 2020

PurposeTo develop a rapid detection reagent for SARS-CoV-2 antigen for the auxiliary diagnosis of new coronary pneumonia (COVID-19), and perform the methodological evaluation and clinical evaluation of the reagent.MethodSARS-CoV-2 N-protein test strip was created by combining fluorescent microsphere labeling technology and immunochromatographic technology, based on the principle of double antibody sandwich. Then we evaluated the analytical capability and clinical application of the strips.ResultThe limit of detection of the strips for recombinant N protein was 100 ng/ml and for activated SARS -CoV-2 virus was 1 × 103 TCID50/ml. The strips also have high analytical specificity and anti-interference capability. According to the predetermined cut-off value, the specificity of the test strip in healthy controls and patients with other respiratory disease was 100.00 and 97.29%, the sensitivity in COVID-19 cases at progress stage and cured stage was 67.15 and 7.02%. The positive percentage agreement and negative percentage agreement of antigen strip to RNA test were 83.16 and 94.45%.ConclusionSARS-CoV-2 fluorescence immunochromatographic test strip can achieve fast, sensitive and accurate detection, which can meet the clinical requirements for rapid detection of viruses on the spot.
Preoperative Imaging Risk Findings for Postoperative New Stroke in Patients With Acute Type A Aortic Dissection
Authors: Zhao Hongliang , Guo Fan , Xu Jingji , Zhu Yuanqiang , Wen Didi , Duan Weixun , Zheng Minwen 
Published: November 2020

Background: Stroke is a common postoperative complication in patients with acute type A aortic dissection (ATAAD). We aimed to explore the preoperative imaging risk findings for postoperative new stroke in patients with ATAAD.Methods: From January 2015 to December 2018, 174 patients with ATAAD who underwent preoperative aortic computed tomography angiography (CTA) and cerebral diffusion-weighted imaging (DWI) as well as postoperative brain CT were included, and divided into DWI (+) and DWI (–) groups. Pre- and intraoperative variables were collected, and logistic regression analysis was used to determine the independent risk predictors of postoperative new stroke.Results: The incidence of postoperative new stroke was 18.4% (32/174) in patients with ATAAD. Postoperative stroke was detected in 13 (31.0%) patients in the DWI (+) group and in 19 (14.4%) patients in the DWI (–) group with significant difference (P = 0.016). In the DWI (+) group, the lesions of the cerebral infarction located in the unilateral cerebral hemisphere and distributed more than three lobes (P = 0.007) were an independent risk factor for postoperative new stroke. Hypotension (P = 0.002), retrograde ascending aorta dissection with thrombosis of the false lumen (P = 0.010), aortic arch entry (P = 0.035), and coronary artery involvement (P = 0.001) were independent risk factors for postoperative stroke in the DWI (–) cohort.Conclusions: Patients with ATAAD with cerebral infarction are more likely to develop postoperative new stroke; thus, a preoperative DWI examination may be necessary. DWI lesions distributed more than 3 lobes in the unilateral hemisphere suggest a high possibility of postoperative stroke. For patients with ATAAD with normal brain, particular attention should be given to the CTA findings of false lumen thrombosis, aortic arch entry, and coronary artery involvement to avoid postoperative stroke.
Potential Role and Prognostic Value of Erythropoietin Levels in Patients With Severe Aortic Stenosis Undergoing Transcatheter Aortic Valve Replacement
Authors: Mas-Peiro Silvia , Seppelt Philipp C. , De Rosa Roberta , Murray Marie-Isabel , Yogarajah Jörg , Berkowitsch Alexander , Fichtlscherer Stephan , Zeiher Andreas M. , Vasa-Nicotera Mariuca 
Published: November 2020

Background: Both EPO levels and anemia have shown prognostic value in several cardiac disorders. An observational study with a prospective follow-up was performed to investigate their independent prognostic roles in severe aortic stenosis.Methods: An up to 36-month follow-up of consecutive patients with severe aortic stenosis undergoing TAVR in a high-volume center was performed. Patients with eGRF &lt;30 mL/min/1.73 m2 were excluded. EPO levels and/or anemia status and its association with mid-term mortality were assessed.Results: Out of 407, 360 met eligibility criteria. Median age was 83 years, with 71.4% having a NYHA class III/IV. Anemia was present in 51.9%, and iron deficiency in 52.8%. Median (IQR) EPO levels were 14.4 (9.30–24.30) mIU/mL. Median follow-up was 566 days. Anemia was associated with overall mortality (HR 2.40, 95% CI 1.51–3.80, p &lt; 0.001). Higher logEPO levels were associated with mid-term mortality (HR 4.05, 95% CI 2.29–7.16, p &lt; 0.001), even after adjusting for clinically and/or statistically relevant factors (multivariate HR 2.25, 95 CI 1.09–4.66, p = 0.029). Kaplan-Meier analyses showed early diverging curves for anemia vs. non-anemia, whereas curves for patients in various EPO level quartiles started to diverge at about 100 days, with differences consistently increasing during the subsequent entire follow-up period.Conclusions: Differently from anemia, which was a strong predictor for both early and late mortality in severe aortic stenosis after TAVR, independent prognostic value of EPO only emerged after post-TAVR recovery. EPO prognostic value was independent from anemia and mild-to-moderate renal dysfunction. High EPO levels could be useful to identify patients with severe aortic stenosis showing a compromised mid-term survival in spite of TAVR use and independently from early TAVR results.
Osteopontin and LDLR Are Upregulated in Hearts of Sudden Cardiac Death Victims With Heart Failure With Preserved Ejection Fraction and Diabetes Mellitus
Authors: Patel Mausam , Rodriguez Daniela , Yousefi Keyvan , John-Williams Krista , Mendez Armando J. , Goldberg Ronald B. , Lymperopoulos Anastasios , Tamariz Leonardo J. , Goldberger Jeffrey J. , Myerburg Robert J. , Junttila Juhani , Shehadeh Lina A. 
Published: November 2020

Background: Diabetes mellitus (DM) is associated with increased risk of sudden cardiac death (SCD), particularly in patients with heart failure with preserved ejection fraction (HFpEF). However, there are no known biomarkers in the population with DM and HFpEF to predict SCD risk.Objectives: This study was designed to test the hypothesis that osteopontin (OPN) and some proteins previously correlated with OPN, low-density lipoprotein receptor (LDLR), dynamin 2 (DNM2), fibronectin-1 (FN1), and 2-oxoglutarate dehydrogenase-like (OGDHL), are potential risk markers for SCD, and may reflect modifiable molecular pathways in patients with DM and HFpEF.Methods: Heart tissues were obtained at autopsy from 9 SCD victims with DM and HFpEF and 10 age and gender-matched accidental death control subjects from a Finnish SCD registry and analyzed for the expression of OPN and correlated proteins, including LDLR, DNM2, FN1, and OGDHL by immunohistochemistry.Results: We observed a significant upregulation in the expression of OPN, LDLR, and FN1, and a marked downregulation of DNM2 in heart tissues of SCD victims with DM and HFpEF as compared to control subjects (p &lt; 0.01).Conclusions: The dysregulated protein expression of OPN, LDLR, FN1, and DNM2 in patients with DM and HFpEF who experienced SCD provides novel potential modifiable molecular pathways that may be implicated in the pathogenesis of SCD in these patients. Since secreted OPN and soluble LDLR can be measured in plasma, these results support the value of further prospective studies to assess the predictive value of these plasma biomarkers and to determine whether tuning expression levels of OPN and LDLR alters SCD risk in patients with DM and HFpEF.
Impact of Comorbidities and Frailty on Early Shunt Failure in Geriatric Patients With Normal Pressure Hydrocephalus
Authors: Hadjiathanasiou Alexis , Kilinc Fatma , Behmanesh Bedjan , Bernstock Joshua , Güresir Erdem , Heimann Muriel , Konczalla Jürgen , Scharnböck Elisa , Schneider Matthias , Weinhold Leonie , Seifert Volker , Vatter Hartmut , Gessler Florian , Schuss Patrick 
Published: November 2020

Background/Aim: Older patients are considered to bear a higher perioperative risk. Since idiopathic normal pressure hydrocephalus (NPH) predominantly concerns older patients, identifying risk factors for early shunt failure for preoperative risk/benefit assessment is indispensable for indication and/or consultation of patients for ventriculoperitoneal shunting (VPS).Methods: We performed a retrospective study design, including data acquired from two university hospital neurosurgical institutions between 2012 and 2019. Overall, 211 consecutive patients with clinical/radiological signs for NPH who additionally showed alleviation of symptoms after lumbar cerebrospinal fluid (CSF) drainage, received VPS and were included for further analysis. Frailty was measured using the Clinical Frailty Scale (CFS). Main outcome was early shunt failure or post-operative complications within 30 days after initial VPS surgery.Results: The overall complication rate was 14%. Patient-related complications were observed in 13 patients (6%) and procedure-related complications in 16 patients (8%). Early post-operative complications resulted in a significantly prolonged length of hospital stay 6.9 ± 6.8 vs. 10.8 ± 11.8 days (p = 0.03). Diabetes mellitus with end-organ damage (OR 35.4, 95% CI 6.6 – 189.4, p &lt; 0.0001) as well as preexisting Parkinson's disease were associated with early patient-related post-surgical complications after VPS for NPH.Conclusions: Patients comorbidities but not frailty were associated with early post-operative patient-related complications in patients suffering NPH. While frailty may deter patients from other (neurosurgical) procedures, VPS surgery might contribute to treating NPH in these patients at a tolerable risk.
Sporadic Creutzfeldt-Jakob Disease and Other Proteinopathies in Comorbidity
Authors: Parobkova Eva , van der Zee Julie , Dillen Lubina , Van Broeckhoven Christine , Rusina Robert , Matej Radoslav 
Published: November 2020

Background: Sporadic Creutzfeldt–Jakob disease (sCJD) is the most common type of a group of transmissible spongiform encephalopathies (prion diseases). The etiology of the sporadic form of CJD is still unclear. sCJD can occur in combination with other neurodegenerative diseases, which further complicates the diagnosis. Alzheimer's disease (AD), e.g., is often seen in conjunction with sCJD.Method: In this study, we performed a systematic analysis of 15 genes related to the most important neurodegenerative diseases - AD, frontotemporal dementia, amyotrophic lateral sclerosis, prion disease, and Parkinson's disease - in a cohort of sCJD and sCJD in comorbidity with AD and primary age-related proteinopathy (PART). A total of 30 neuropathologically verified cases of sCJD with and without additional proteinopathies were included in the study. In addition, we compared microtubule-associated protein tau (MAPT) haplotypes between sCJD patients and patients with sCJD and PART or sCJD and AD. Then we studied the interaction between the Apolipoprotein E gene (APOE) and PRNP in sCJD patients.Results: We did not find any causal mutations in the neurodegenerative disease genes. We did detect a p.E318G missense variant of uncertain significance (VUS) in PSEN1 in three patients. In PRNP, we also found a previously described non-pathogenic insertion (p.P84_Q91Q).Conclusion: Our pilot study failed to find any critical differences between pure sCJD and sCJD in conjunction with other comorbid neurodegenerative diseases. Further investigations are needed to better understand this phenomenon.
Competing Risks of Cancer and Non-Cancer Mortality When Accompanied by Lifestyle-Related Factors—A Prospective Cohort Study in Middle-Aged and Older Adults
Authors: Macek Pawel , Biskup Malgorzata , Terek-Derszniak Malgorzata , Manczuk Marta , Krol Halina , Naszydlowska Edyta , Smok-Kalwat Jolanta , Gozdz Stanislaw , Zak Marek 
Published: November 2020

BackgroundThe study aimed to identify the association between the lifestyle-related factors and the cancer-specific, or non-cancer-specific mortality, when accompanied by a competing risk. Two statistical methods were applied, i.e., cause-specific hazard (CSH), and sub-distribution hazard ratio (SHR). Their respective key advantages, relative to the actual study design, were addressed, as was overall application potential.MethodsSource data from 4,584 residents (34.2% men), aged 45–64 years, were processed using two different families of regression models, i.e., CSH and SHR; principal focus upon the impact of lifestyle-related factors on the competing risk of cancer and non-cancer mortality. The results were presented as hazard ratios (HR) with 95% confidence intervals (95% CI).ResultsAge, smoking status, and family history of cancer were found the leading risk factors for cancer death; the risk of non-cancer death higher in the elderly, and smoking individuals. Non-cancer mortality was strongly associated with obesity and hypertension. Moderate to vigorous physical activity decreased the risk of death caused by cancer and non-cancer causes.ConclusionsSpecific, lifestyle-related factors, instrumental in increasing overall, and cancer-specific mortality, are modifiable through health-promoting, individually pursued physical activities. Regular monitoring of such health-awareness boosting pursuits seems viable in terms of public health policy making.
The Prognostic Value of Androgen Receptor Splice Variant 7 in Castration-Resistant Prostate Cancer Treated With Novel Hormonal Therapy or Chemotherapy: A Systematic Review and Meta-analysis
Authors: Wang Zhize , Shen Haixiang , Ma Nieying , Li Qinchen , Mao Yeqing , Wang Chaojun , Xie Liping 
Published: November 2020

PurposeThis study aimed to evaluate the prognostic role of AR-V7 in terms of prostate-specific antigen (PSA) response, progression-free survival (PFS), and overall survival (OS) in CRPC patients treated with novel hormonal therapy (NHT) (Abiraterone and Enzalutamide) or taxane-based chemotherapy (Docetaxel and Cabazitaxel).MethodsA comprehensive literature search was conducted on PubMed, Embase, and the Web of Science from inception to February 2020. Studies focusing on the prognostic values of AR-V7 in CRPC patients treated with NHT or chemotherapy were included in our meta-analysis. The OS and PFS were analyzed based on Hazard ratios (HRs) and 95% confidence intervals (CIs). Furthermore, Odds ratios (ORs) and 95% CIs were summarized for the AR-V7 conversion after treatment and the PSA response.ResultsThe AR-V7 positive proportion increased significantly after NHT treatment (OR 2.56, 95% CI 1.51–4.32, P&lt;0.001), however, it declined after chemotherapy (OR 0.51, 95% CI 0.28–0.93, P=0.003). AR-V7-positive patients showed a significantly decreased PSA response rate after NHT (OR 0.13, 95% CI 0.09–0.19, P&lt;0.001) but not statistically significant for chemotherapy (OR 0.63, 95% CI 0.40-1.01, P=0.06). Notably, PFS (HR 3.56, 95% CI 2.53–5.01, P&lt;0.001) and OS (HR 4.47, 95% CI 3.03–6.59, P&lt;0.001) were worse in AR-V7-positive ttreated with NHT. Similarly, AR-V7 positivity correlated with poor prognosis after chemotherapy as evidenced by shorter OS (HR 1.98, 95% CI 1.48-2.66, P&lt;0.001) and a significantly shorter PFS (HR 1.35, 95% CI 0.97-1.87, P=0.07).ConclusionNHT treatment increased AR-V7 positive proportion whereas chemotherapy decreased it. Moreover, AR-V7 positivity correlated with lower PSA response, poorer PFS, and OS in CRPC treated with NHT, and shorter OS in patients receiving chemotherapy.