bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of storage on viability of lactic acid bacteria and nutritional stability of raw Malaysian Heterotrigona itama honey
Authors: Yaacob S N S , Wahab R A , Huyop F , Ibrahim R K R , Zin N M 
Published: July 2020

Abstract
The nutritional benefits of stingless bee (Heterotrigona itama) honey originates from a complex of essential nutrients including carbohydrates, organic acids alongside an assortment of live lactic acid bacteria (LAB). Nonetheless, little is known about the nutritional composition over an extended storage duration. Herein, this study assessed the nutritional contents in Malaysian raw H. itama honey in response to changes in viability of LAB over 28 days of storage. LAB total count was monitored against quality parameters viz. pH, total flavonoid, total phenolic and antioxidant scavenging activity. Results revealed that LAB population was no longer detected after day 28 days of storage (p < 0.05) as compared to initial count of 2.62 × 105 CFU/g. The study recorded minor changes in pH that was reduced from pH 2.96 to pH 2.86. Notably, relatively stable (p > 0.05) total flavonoid (36-60 mg QEA/100 g) and total phenolic contents (38-62 mg GAE/100 g), with antioxidant scavenging activity (IC50 between 24-45 mg/mL) were detected for raw H. itama honey. It was irrefutably demonstrated that 28 days of storage does little to depreciate the nutritive value of Malaysian raw H. itama honey, despite the appreciable decline in live LAB counts.
Mutation identification of unreported and reported mutation profile in exon 7 of N-Acetylgalactosamine-6-Sulfates (GALNS) gene of mucopolysaccharidosis type IV A (MPS IVA) patients in Indonesia
Authors: Lestari I C , Priambodo R , Ariani Y , Hafifah C N , Abinawanto  , Sjarif D R 
Published: July 2020

Abstract
Mucopolysaccharidosis type IVA (MPS IVA), is an autosomal recessive genetic disorder because of N-acetylgalactosamine-6-sulfate deficiency which causes keratan sulfate and chondroitin sulfate to not degrade in lysosome. MPS IVA was caused of GALNS gene which located in chromosome 16q24.3 with the most frequent mutation occurrence in exon 7. There are no report or publication about MPS IVA or GALNS gene mutation in Indonesia, therefore this research is aimed to analyze the mutation profile in exon 7 of the GALNS gene in MPS IVA patients in Indonesia. The DNA from blood samples of four patients and three control samples from RSUPN Cipto Mangunkusumo were analyzed. Amplification by polymerase chain reaction was done after designing the primer. Furthermore, electrophoresis and sequencing analysis has been performed. The result shows that there is silent mutation c.708C>T and nonsense mutation c.751C>T. The silent mutation is categorized as a benign variant, while the nonsense mutation is categorized as a pathogenic variant because it may affect protein features and cause neuromuscular disorder. The silent and nonsense mutation that were found were already reported by Laradi and Morrone, but has not been reported in Indonesia. Further experiment was needed to find other mutation in other genes.
Analysis of malondialdehyde and superoxide dismustase levels after exposure of electric cigarette in rats
Authors: Lisdiana  , Nugrahaningsih WH , Nufus I 
Published: July 2020

Abstract
Electric cigarette (e-cigarette) is cigarette that operate on battery power to burn liquids and to produce a steam. One of the contents of an electric cigarette is nicotine. It is a chemical compound that can cause addiction and trigger oxidative stress. This study aims to analyze the levels of malondialdehyde and superoxide dismutase in the blood of rats that exposed to nicotine from e-cigarettes. The study was conducted on 30 male Wistar rats which divided into 5 groups, control group there are negative and positive and treatment group with nicotine 0,25 mg, 0,5 mg, 0,75mg with exposure to cigarette smoke for 30 days. Malondialdehyde and superoxide dismutase levels measurements using the TBARs method, the results that can be known using a spectrophotometer with a wavelength of 532 nm. SOD levels were measured by the calorimetry method. The One Way Anova analysis showed that malondialdehyde and superoxide dismutase levels in the control group were significantly different from all groups. The conclusion is that nicotine has an effect on increasing malondialdehyde and decreasing superoxide dismutase levels.
The correlation between total antioxidant status with malondialdehyde and caspase 3 epididymal fluid type 2 diabetic rat and non diabetic
Authors: Christijanti W , Juniarto A Z , Suromo L B 
Published: July 2020

Abstract
The aim of this study is to determine the correlation between total antioxidant status (TAC) with malondialdehyde (MDA) and caspase 3. This study was divided into non-diabetic (n = 8) and type 2 diabetic rats (n = 8) induced by streptozotocin 65 mg / kg body weight. Epididymal fluid was examined after 28 days. Antioxidant status was examined by the trolox equivalent method, MDA with the thiobarbituric acid (TBA) reaction and caspase 3 expression with immunohistochemistry. Our results show that the mean of TAC in non-diabetic was significantly higher (P <0.000) than diabetic rats, MDA (P <0.000) and caspase 3 expression (P <0.001) in non-diabetic rats were significantly lower than diabetic rats. TAC was negative correlation with MDA (r = 0.881, P = 0.004) and caspase 3 (r = 0.898, P = 0.002) in the non-diabetic group. In the diabetic group, TAC was a negative correlation with MDA (r = 0.856, P = 0.007) and caspase 3 (r = 0.886, P = 0.003). In conclusion, total antioxidants have a negative correlation with MDA and caspase 3 expression.
The potential effect of high flavonoid soybean diversification products through tempe flour substitution
Authors: Bintari S H , Putri M F , Saputro D D , Suwahyo  , Parman S , Sunyoto  
Published: July 2020

Abstract
Nowadays, overconsumption of junk food and sedentary lifestyle lead to the development of various diseases. Legumes intake has been known to support one’s health since it’s contain various nutrients and bioactive compounds that’s beneficial for health. One of the most consumed legumes is soybean. In Indonesia, soybean usually consumed as tempe and tahu (tofu). Tempe contain numerous nutrients, for example carbohydrates, fats, proteins, minerals, dietary fibers, vitamins, and antioxidants. Therefore, tempe can be eaten as it is of added to other food or to create a new food product through diversification. This study aims to characterize the diversification product of tempe on antioxidant and nutritional content. The diversification products are tempe flour (TF), tempe meatball (TM), chocolate tempe (CT), tempe moringa (TM), and tempe onion (TO). The new products still have the same basic flavor as before, but the chemical tests show there are a number of flavonoid compounds increasing with highest concentration found in TF. In conclusion, TF can be use in the future for further diversification to create tempe-based food with high antioxidant content.
Effects of Chitosan on Sperm Quality of Lead Acetate-Induced Rats
Authors: Marianti A , Isnaeni W , Setiati N , Sumadi S 
Published: July 2020

Abstract
Escalation in blood lead level may decrease sperm quality. Chelating agent, such as chitosan, is known to reduce the detrimental effects from lead exposure. This research aims to analyze the effect of chitosan on sperm quality of lead acetate-induced rats. 30 rats were divided into six groups. All treatment groups, aside from control group, were induced by 175 mg/kg weight of lead acetate. Positive control group was treated with Ca2EDTA, while negative control group was only treated with lead acetate. Treatment group 1, 2, and 3 were treated with chitosan in gradual dose; 64, 32, and 16 mg/kg weight respectively. On the 31st day, the sperm quality was analyzed and the rats were terminated. Statistical result showed that positive control group and treatment groups had no significant difference from control group but was significantly different from negative control group. Research parameters, which were sperm count, motility of progressive sperm, immotile sperm, and normal sperm, were significantly different among the all treatment groups. However, another research parameter, which were nonprogressive sperm motility and sperm viability were not significantly different. Treatment group 1 was not different significantly from control and positive control groups. To sum up, chitosan improves the sperm quality of lead acetate-induced rats.
Morphological characterization and its variation of “Wirasangka” mango as the flora identity of Tegal regency, Central Java, Indonesia
Authors: Abdullah M , Rahayu E S , Retnoningsih A 
Published: July 2020

Abstract
“Wirasangka” mango (Mangifera indica L. var. Wirasangka) is a flora of identity from Tegal Regency, Central Java Province. There is a difference in understanding about Wirasangka mangoes among the people. Some residents mentioned that “wirasangka” mango is another name for golek, honey golek, Indian golek, okyong, and tengkoeh mangoes; while others claim that the varieties differ from one another. Therefore it is necessary to do scientific characterization to ensure the truth of the “Wirasangka” mango cultivar. The purpose of this research was to characterize the Wirasangka mango based on morphological markers and examine variations in vegetative characteristics. The research was conducted on “Wirasangka” mango habitat in several areas of Tegal Regency, Central Java. Plant material used were trees referred to by local residents as “Wirasangka” mango, golek, honey golek, Indian golek, okyong, and tengkoeh. Mango trees planted by Tegal Regency Agriculture Department employees in the Procot area were used as a reference for Wirasangka mangoes. Morphological character identification was carried out based on the Descriptor handbook for Mangoes (Mangifera indica) from the International Plant Genetic Resources Institute. Data were analyzed using the R statistical program to calculate the coefficient of variation of its vegetative characteristics and research the comparison of characters between cultivars. Based on observations of morphological character measurements, the results show that there are low morphological variations among the Wirasangka mango cultivars. The results of the dendogram cluster analysis show that the cultivar referred to by the community as the Wirasangka mango has a high similarity with the cultivar golek, the Indian golek, and okyong.
Using two tier based concept test to analysis profile of student understanding on the concept of simple harmonic motion
Authors: Umam A , Suparmi  , Sukarmin  
Published: July 2020

Abstract
The purpose of this study was to analyze students understanding profile about simple harmonic motion conceptual. The subject of this study were eleventh grade student of sciences class in Surakarta2 State Senior High School with 90 students whose registered as student in the 2018/2019 school year. Sampling in this study used random sampling technique. To analyze students understanding profile used instrument of two tier test with 20 item. Instrument consists two level, first level is multiple choice questions and the second level is open ended free response question which is the reason of student’s answer to the first level questions. The result of this study show that 12,9% of students understanding the concept of simple harmonic motion, 16% of students understand in part, 18,2% of students show misconception, 31,5% of students show specific misconception, and 16,5% of students didn’t understand the concept. The highest concept understand was in concept relation of velocity, angular velocity, displacement and acceleration in simple harmonic motion with 41,11%, and the lowest was in definition of simple harmonic motion with 0,74%.
Framework TPACK using Quick Response (QR) code to promote ICT literacy students in learning physics
Authors: Oktasari D , Jumadi  , Warsono  , Putri Z R 
Published: July 2020

Abstract
Teachers are an important instrument in finding a new role that is more contextual and relevant to 21st century learning concepts offered a demanding professionalism of teachers in integrating technology, pedagogy and content knowledge (TPACK) in learning. This research aims to design a learning device in accordance with the framework using technology TPACK Quick Response (QR) Code to promote ICT literacy skills of students. The subjects were 95 students menangah school (high school) class X at SMAN 6 Yogyakarta. These results indicate that the instrument developed ICT literacy test had a reliability 0.84. Criteria for each item has a value of 0.98 with the criteria infit meansquare grains fit with the model of Rasch (meansquare infit ≤ 0.77 ≤ 1.33) with both criteria. Cohen’s interpretation of the value of f at 0:33 in the category of large effect size. TPACK Framework Using Quick Response (QR) Code that was developed has a positive effect with the high category in enhancing ICT literacy of students.
The Implementation of collaborative learning models using worksheet to increase student learning outcomes at senior high school the subject of light waves
Authors: Hermarini R , Astra I M , Budi A S 
Published: July 2020

Abstract
This action research aims to increase the learning outcomes of students by implementing of collaborative learning models using worksheet on the subject of light waves in SMAN 2 Indramayu XI-1 Mathematics and Natural Sciences. The research models used in this study is action research that actions applied with the following stages: the first stage of planning; the second stage of acting; third stage of observing; fourth stage of reflecting. This study consisted of three cycles implemented in January 2019. The instruments of this study are worksheet, observation sheets of teacher and student activities and learning outcomes assessment sheets which consist of cognitive, psychomotor and affective assessments. Based on the results of research and discussion of students learning outcomesgradually in each cycle, the conclusions obtained in this study that by implementing of collaborative learning models using worksheet to increase students learning outcomes at senior high school on the subject of light waves.
Developing of physics teaching materials based on authentic learning to train problem-solving skills
Authors: Wati M , Sutiniasih N , Misbah  , Mahtari S , Annur S , Mastuang  
Published: July 2020

Abstract
Authentic learning as a means that allows students to develop knowledge transferred to real-world practice. This research aimed at determining the validity, the practicality and the effectivity of the use of physics teaching materials based on authentic learning. This research type is a research and development using the ADDIE model. The subjects of the trial were 33 students of class X at one of the high schools in Banjarmasin. The research instruments were validation sheets, observation sheets for lesson plan implementation, student achievement results and problem-solving skills assessment sheets. The results showed that: (1) the validity of the Physics teaching materials based on authentic learning was very high with an average value of 3.68, (2) practicality with an average of 3.68 criteria very high, (3) effective in improving aspects of knowledge of students who are classified as moderate with a gain score of 0.6 and problem-solving skills is adequately trained. It was concluded that the physics teaching materials based on authentic learning were declared valid, practical and effective to train problem-solving skills. This problem-solving skills is also useful for students to get success in real life.
The critical thinking ability profile of grade X SMA N 2 Kudus
Authors: Rahayu E C , Suparmi  , Sarwanto  
Published: July 2020

Abstract
This study aims to describe the critical thinking ability of grade X students of SMAN 2 Kudus on physics subjects. The design of this study uses quantitative descriptive methods. The research was conducted at SMAN 2 Kudus with a sample of class X research taken by cluster random sampling. The instrument used was a test of critical thinking skills. Data analysis was carried out in a quantitative percentage. The results showed that critical thinking skills of class X students of SMAN 2 Kudus were in the low ability category with an average percentage of 22.91 %.
Examining the relationship between students’ motivation and critical thinking skills in learning torque and static equilibrium.
Authors: Andriani R , Hidayat A , Supriana E , Anantanukulwong R 
Published: July 2020

Abstract
This study designed to determine the motivation components that most motivated students in learning torque and static equilibrium and the relationship of the motivation with students’ critical thinking skills on Torque and Static Equilibrium subject. The research was conduct in Senior High Scholl Laboratory State University of Malang. This study used correlational research design and using the Physics Motivation Questionnaire II (PMQ II) and Torque and Static Equilibrium Critical Thinking test as Instrument. The analysis showed that students have a weak motivation level in learning torque and statistic equilibrium. Grade motivation found to be the most motivating for students. The least motivated for students is Career Motivation. Researcher found that critical thinking skills correlate significantly with intrinsic motivation, grade motivation, and self-efficacy, but the relationship between critical thinking skills with self-determination and career motivation were not significant. However, the overall motivation has a significant correlation with students’ critical thinking skills.
Generic skills pattern of physical teacher’s candidate through design of school physics practicum guidelines
Authors: Khoiri N , Rusilowati A , Wiyanto  , Sulhadi  , Jafar R 
Published: July 2020

Abstract
The aim of the study is to describe the patterns of generic thinking skills of students through the design of physics practicum guidelines. The research method uses quasi-experimental using post-test only control group design. The research sample is a Physics Education study program student in 5th semester, taken by cluster random sampling technique. The experimental group carried out physics laboratory project learning with generic science skills while the control group studied physics laboratory projects. Data collection techniques are using observation and test sheets. Data analysis techniques using statistical tests with t test. The results of the study obtained observational data and test data, the data were tested for normality, reliability and statistical tests. The difference in generic science skill patterns was tested statistically, and then data were analyzed for each indicator of generic science skills. The product of this research is the school physics practicum guidelines. The results of the analysis of the generic science skills test showed that the generic science skills in the experimental class were better than the control class. Based on the generic science skill pattern, it is found that the physics teacher candidates are better on the aspects of direct and indirect observation.
Validation construct: Confirmatory Factor Analysis (CFA) instruments scientific communication skills students in learning physics
Authors: Oktasari Depi , Ismet  , Siahaan S M 
Published: July 2020

Abstract
Scientific communication skills into competencies required of students in the 21st century scientific communication must be owned that is orally or in writing. This research aims to develop assessment instruments scientific communication skills of students in physics. Scientific communication skills assessment instrument is a synthesis developed into five indicators. Instruments tested on students of SMAN 6 Yogyakarta. The instrument was developed to test the construct using LISREL. LISREL analysis used in the form Confirmatory Factor Analysis (CFA) to see how the fit between indicators developed models. The results showed that scientific communication instrument had a reliability 0.82. Criteria for each item has a value infit meansquare 1:01 with grain criteria fit with the model of Rasch (meansquare infit ≤ 0.77 ≤ 1.33) with both criteria. Loading factor of each item instrument has a value of> 0.3. The results validate the results obtained construct second order confirmatory factor analysis (CFA) on 17 items results showed that p-value = 0.27505 (p> 0.05) and RMSEA = 0.031 (RMSEA> 0.05). By loading factor> 0.3 and t-value> 1.95 so that the item is valid and thus fit modelDengan scientific instruments developed communication skills can be used to measure scientific communication skills of students in physics.
Physics based learning effectiveness PhET simulation model using Problem Based Learning (PBL) for self-independent learning on material and energy enterprises learners MAN 3 Sleman
Authors: Saputra S , Jumadi  , Wilujeng I 
Published: July 2020

Abstract
This study aims to determine the effectiveness of the use of Phet simulation applications on smartphones Problem Based Learning (PBL) learning models of students’ learning independence and the different effects of learning independence. The type of research applied was quasi experiment. Research design used Non-equivalent Control Group Design. The population in this study was all students of class X of MAN 3 Sleman which was divided into 8 classes. The sampling technique used cluster purposive sampling. Both classes are given treatment with the Problem Based Learning model assisted by the Phet application which was installed on each student’s smartphone. The average level of learning independence of students in the modelling class after they participated in learning activities included in the good category, and in the implementation, class included in the good category. The significance level shows the numbers in the effective category. So it can be stated that the learning independence questionnaire of both classes was effective, it can be concluded that the Problem Based Learning model assisted by the Phets application is effective in terms of the achievement of the learning independence of students in class X of MAN 3 Sleman in the academic year 2017/2018.
A problem of chemistry book: The need to improve student’s science literacy with STSE approach
Authors: Lestari O , Anwar S , Priscylio G , Agustina N R , Wahyuni W S , Oktasari C 
Published: July 2020

Abstract
In this article, we explain the need for chemistry book developed by 4S TMD models on the level of science and literacy with STSE approach. Teaching materials are one of the determinants in the chemistry learning process, one of them is the concept of rate reaction. Rate reaction concept there are many applications in daily life. One suitable approach to use is the STSE approach. Teaching materials with the STSE approach help students reflect on the scientific, environmental, technological and social aspects of learning. Science literacy as an output of knowledge, and scientific processes. The instruments for data collection used in this study were structured questions and interviews. This research was conducted in 2 senior high schools, 2 vocational high schools with 12 chemistry teachers. The teacher structure question responses was analyzed descriptively for each item. Teaching materials currently available have not been able to increase students’ interest in reading because their appearance is less attractive, material content is difficult for students to understand. Most teachers want teaching materials that are easily understood by students, full colour, contain many examples in daily life. So that it can improve students’ scientific literacy. STSE-based teaching materials are expected to improve students’ scientific literacy because contain content in life. STSE-based teaching materials are still rarely used because of the lack of availability of STSE-based teaching materials that can be used by teachers in learning. The results of this study are the basis for us in developing chemistry book by 4S TMD models on rate reaction to improve student’s science literacy with STSE approach that can be used in chemistry learning and teaching in upper high schools.