bluuebunny/crossref_metadata_2025_split huggingface dataset:
EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, ANTIHEMOLYTIC, AND PHYOCHEMICAL PROPERTIES OF FICUS BENJAMINA, FICUS INFECTORIA, AND FICUS KRISHNAE
Authors: SINGH JASSAL PRABHJOT , SHARMA MONIKA 
Published: October 2019

Objective: The study was conducted for the evaluation of antioxidant, antibacterial, antihemolytic, and phytochemical activity of Ficus benjamina (FB), Ficus infectoria (FI), and Ficus krishnae (FK).
Methods: The antioxidant analysis of FB, FI, and FK was done by 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and nitric oxide (NO) scavenging assay. Evaluation was performed for antibacterial activity against Clostridium perfringens and Staphylococcus aureus by an agar well diffusion method. Hemolytic assay was performed with red blood cell suspension for evaluating antihemolytic activity. Standard protocols were used for phytochemical activity.
Results: In the results of DPPH antioxidant activity assay, it was found that DPPH inhibition was significantly increased with increasing amount of extract. For NO scavenging assay, ANOVA revealed that the inhibition of NO is not significantly affected with an increase in the amount of extract used. Results of antibacterial activity revealed that methanolic extract of FI shows a maximum zone of inhibition (30.5 mm) against S. aureus and aqueous extract of FK shows a maximum zone of inhibition (28 mm) against C. perfringens. The antihemolytic activity of FB, FI, and FK was performed by measuring percentage inhibition of plant extracts at different concentrations. FK has shown maximum percentage inhibition activity, i.e., 28.64 % at 60 μg/ml, whereas FB shows minimum inhibition activity, i.e. 2.7 % at 40 μg/ml. Flavonoid content was found to be 0.593 μg/ml, 0.783 μg/ml, and 1.023 μg/ml, whereas phenolic content was found to be 0.267 μg/ml, 0.298 μg/ml, and 0.355 μg/ml for FB, FI, and FK, respectively.
Conclusion: FB, FI, and FK extracts contain various phytochemicals which confirm that these plants can be used for therapeutic use and traditional medicine. The methanolic as well as aqueous extracts of the plants have shown the potential to kill the tested microorganism (C. perfringens and S. aureus) and hence can be used as an antibiotic and potential antibacterial.
COMPARATIVE ANALYSIS OF PHYTOCHEMICAL, ANTIBACTERIAL, AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF AZADIRACHTA INDICA LEAVES
Authors: SHARMA GAURAV , THAKUR ANKITA , LAL SOHAN , KUMAR NADDA ROHIT 
Published: October 2019

Objective: The objective of the present study was the analysis of phytochemicals in various extracts of Azadirachta indica leaves, comparative evaluation of antibacterial activity of the various extracts of A. indica leaves against Escherichia coli and Staphylococcus aureus, and comparative evaluation of antioxidant activity in various extracts of A. indica leaves using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay.
Methods: Various extracts were prepared by crushing the samples. Antibacterial susceptibility test, various phytochemical tests for qualitative analysis, and DPPH radical scavenging assay for antioxidant activity were performed.
Results: The result suggested that alkaloids, flavonoids, and terpenoids were present in all the four extracts. Tannins were absent in the ethyl acetate extract, and phenols were only present in the ethyl acetate extract. Sterols and phlobatannins were absent in all the four extracts. Saponins were only present in the aqueous extract, and amino acids were only present in the ethyl acetate extract. The bacterial strains S. aureus and E. coli were used against the different extracts of A. indica leaves, i.e., methanol, chloroform, ethyl acetate, and aqueous.
Conclusion: The results suggested that bioactive compounds found in leaves of A. indica contribute to its pharmacological activities.
COMPOSITION OF THE ESSENTIAL OIL FROM MIMOSA PUDICA LINN.
Authors: TK VISMAYAVISWAN , J DHARANI , R SRIPATHI , S RAVI 
Published: October 2019

Objectives: To determine the composition of the essential oil obtained from Mimosa pudicaÂ L. (Mimosaceae)Â Methods: The essential oilÂ Â of M. pudica was obtained by hydro distillation using a Clevenger apparatus and the chemical composition was determined by GC-MS analysis.Â Results: About 23 compounds were identified from the essential oil which constitutes about 98.92 % of the oil. Among the compounds identified phthalic acid dioctyl ester (27.76 %), Î±-Linolenic acid (20.34 %), Cinnamaldehyde (16.24 %) are the major compounds.Â Î±-Linolenic acid is an n-3 fatty acid.Â Conclusion: Î±-Linolenic acid is one of the essential fatty acids, which is necessary for health and cannot be produced within the human body.Â Mimosa pudicaÂ L. (Mimosaceae) appears to be a promising herb to undergo a wide exploration
HOMOCYSTEINE: A NEWER AND NOVEL INDEPENDENT RISK FACTOR AND CARDIAC MARKER FOR ACUTE MI
Authors: KUMAR ANOOP , SHARMA PREETI , KUMAR PRADEEP , KUMAR DR ASHOK 
Published: October 2019

Objective: The objective of this study was to estimate homocysteine levels in myocardial infarction (MI) patients (troponin-T positive) and normal healthy individuals (troponin-T negative) subsequently to make comparisons with other cardiac markers.
Methods: A cross-sectional study consisting of 172 subjects involving 100 patients of non-diabetic MI and 72 apparently healthy controls with no history of diabetes and/or MI was done between December 2017 and May 2018. The diagnosis of MI was established with electrocardiogram findings and troponin-T estimation. Blood samples were collected and processed for the estimation of homocysteine, troponin-T, creatine kinase MB fraction (CK-MB), and lactate dehydrogenase.
Results: In the present study, a total of 100 troponin-T positive cases and 72 troponin-T negative as controls were studied. The mean age in the cases was 62.15±7.75 years and in the controls was 61.49±8.35 years (p=0.592). The mean value of homocysteine in the troponin-T positive group was 30.56±19.79 μmol/l and in the troponin-T negative group was 10.28±4.03 μmol/l (p<0.0001). Homocysteine was deranged in 98% troponin-T positive group and in only 18.06% in troponin-T negative group. The difference was statistically significant (p<0.0001). No significant correlation was found between homocysteine and CK-MB and LDH in troponin-T positive and negative patients individually.
Conclusion: This study indicated that homocysteine is sensitive cardiac markers for the diagnosis of MI but shows no specific correlation with other cardiac markers and thus it should be predicted independently.
IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF THE FLOWER EXTRACTS OF OPUNTIA STRICTA
Authors: D PRABHAKARAN , A RAJESHKANNA , MM SENTHAMILSELVI 
Published: October 2019

Objective: The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of the solid powder obtained from the ethyl acetate fraction from the flower Opuntia stricta.
Methods: The flower extract was evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and reducing power assay was carried out by ferric-reducing capacity (FRC) assay method. The in vitro anti-inflammatory activity was evaluated using human peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO) production method.
Results: The solid powder obtained from the ethyl acetate fraction from the flower O. stricta showed a good antioxidant activity in scavenging DPPH radical and FRC assay with compared standard sample. This solid powder also showed good anti-inflammatory activity in cell viability (LPS-induced PBMCs) assay and NO assay.
Conclusion: These results suggest that the solid powder obtained from the ethyl acetate fraction from the flower O. stricta has significant antioxidant and anti-inflammatory activities.
A PROSPECTIVE STUDY ON DRUG UTILIZATION PATTERN OF ANTIDEPRESSANTS IN NON-PSYCHIATRIC DEPARTMENTS IN A TERTIARY CARE TEACHING HOSPITAL
Authors: SEBASTIAN ANCY , PK ANEESHA , AREECKAL JOSHWA , DAVIS SONIYA 
Published: October 2019

Objective: The aim of this study is to evaluate the pattern of prescription and utilization of antidepressants (ADs) for various non-psychiatric indications in department of general medicine, cardiology, surgery, gynecology, and orthopaedic of a tertiary care teaching hospital.
Methods: A prospective cross-sectional unicentric drug utilization study of inpatients was carried out in a 450 bedded tertiary care hospital. The study included 200 subjects admitted for both psychiatric and non-psychiatric illness. Pediatric patients and those with a length of stay <48 h were excluded from the study.
Results: Prescriptions of 200 patients were studied in which 55% were males and 45% were females. The most widely prescribed drug is alprazolam comprising 50% of the total drug consumption and is followed by clonazepam (19.5%), amitriptyline (13.5%), diazepam (9.5%), escitalopram (3.5%), lorazepam (3%), and zolpidem (0.5%). About 36% of the drugs were prescribed in the general medicine department, and 27.5% of the prescriptions were from the cardiology department. Prescriptions from surgery, orthopedics, and gynecology were 16%, 11.5%, and 9% of the total, respectively.
Conclusion: ADs were found to be potentially useful for non-psychiatric indications such as peripheral and diabetic neuropathy, gastroenterological conditions, and urological conditions. No benefit was observed in insomnia and musculoskeletal conditions except for fibromyalgia. ADs were also found to improve the treatment outcome and quality of life of patients having comorbid psychiatric conditions being treated for non-psychiatric disorders.
ANTI-INFLAMMATORY ACTIVITY OF NEURACANTHUS SPHAEROSTACHYUS DALZ. LEAVES
Authors: DK DANGAR , NJ PATEL 
Published: October 2019

Objective: Neuracanthus sphaerostachyus has been traditionally used to treat skin diseases, cough, and asthma. Lack of sufficient scientific evidence indicating the utility of this plant in the treatment of inflammation prompted us to investigate the anti-inflammatory activity of the plant in different experimental screening methods.
Methods: In vitro and in vivo anti-inflammatory activity of the methanolic and aqueous extracts of N. sphaerostachyus (MENS and AENS) leaves at doses of 125, 250, and 500 mg/kg was evaluated with albumin denaturation and carrageenan-induced paw edema in rats and acetic acid-induced increased vascular permeability in mice.
Results: Methanolic and aqueous extract significantly inhibited protein denaturation as well as edema induced by carrageenan and vascular permeability in mice dose dependently. Aspirin (0.1 mg/ml), indomethacin (10 mg/kg), and dexamethasone (5 mg/kg) were used as a standard control.
Conclusion: It is concluded that MENS and AENS leaves exhibited significant anti-inflammatory activity.
PHYTOCHEMICAL EXTRACTIONS, QUALITATIVE ANALYSIS, AND EVALUATION OF ANTIMICROBIAL ACTIVITY IN THE LEAF AND STEM BARK OF SOLANUM PUBESCENS WILLD.
Authors: VN SUDHAMA , M RAMAKRISHNAN 
Published: October 2019

Objective: The objective of this study was to extract the phytochemicals from leaf and stem bark of Solanum pubescens, perform qualitative analysis, and evaluate antimicrobial activity of the phytochemicals against certain microbial pathogens.
Methods: Leaf and stem bark of S. pubescens was subjected to phytochemical extractions using Soxhlet apparatus with five different solvents, identified the major constituents in different solvent extracts using standard protocols and investigated the potential antimicrobial activities of the extracts against certain selected bacterial and fungal pathogens by agar well diffusion method.
Results: The maximum yield was in methanolic extracts of leaf and stem bark of the study plant such as 18.51 and 12.5%, respectively, followed by the hydroalcohol extracts (14.23 and 10.00%). Qualitative phytochemical analysis revealed maximum number of extracted bioactive compounds when compared to other similar studies. Among the five different solvent extracts of S. pubescens, n-hexane extract of stem bark was found to be active against all the bacterial pathogens and stem bark extracts made with methanol and hydroalcohol showed antifungal activity against all the four fungal pathogens tested. Leaf extracts made with solvents such as n-hexane and ethyl acetate showed inhibition against Klebsiella pneumoniae and Staphylococcus aureus, respectively, hydroalcohol extract was active against Pseudomonas aeruginosa and Salmonella typhimurium while none of the leaf extracts of the study plant showed inhibition zones against fungal pathogens tested.
Conclusion: Multiple solvent extraction approach yielded the phytochemicals which are not yet reported and the antimicrobial activities of phytochemicals acknowledged their medicinal value.
HEPATOPROTECTIVE AND NEPHROPROTECTIVE EFFECTS OF THE AQUEOUS EXTRACT OF TURMERIC (CURCUMA LONGA) IN RIFAMPICIN AND ISONIAZID-INDUCED HEPATOTOXICITY AND NEPHROTOXICITY IN RATS
Authors: M THUAWAINI MAHDI , AL-FARHAAN MAWAHIB B GASIM , F ABBAS KARIMA 
Published: October 2019

Objectives: The present study was designed to estimate the influences of oral administration of aqueous extract of turmeric (Curcuma longa) in hepatotoxicity and nephrotoxicity induced in rats by isoniazid and rifampicin (RIF) for 4 weeks. Influences were determined through the estimation of liver and kidney functions and histopathological changes.
Materials and Methods: A total of 48 male albino rats were randomly divided into six groups: Normal control, INH+RIF treated rats, Turmeric aqueous extract 100 mg/kg treated rats, Turmeric aqueous extract 100 mg/kg + INH and RIF treated rats, Turmeric aqueous extract 200 mg/kg treated rats, Turmeric aqueous extract 200 mg/kg+ INH and RIF treated rats. Turmeric aqueous extract and INH + RIF (50 mg/kg bwpo, daily) were given for 4 weeks. Liver and kidney function markers (aspartate transaminase [AST], alanine transaminase [ALT], alanine phosphatase [ALP], bilirubin, blood urea, and creatinine) were determined enzymatically. In addition, tissues of liver and kidney were quickly separated and fixed in 10% formalin and subjected to histopathological studies. Statistical analysis was carried out using t-test.
Results: The aqueous extract of turmeric (at a dose of 100 and 200 mg/kg bw, p.o. daily ) showed hepato- and reno-protective effects in hepato- and reno- toxicity induced by RIF and INH in rats. Significant elevation of serum ALT, AST, ALP, total bilirubin, creatinine, urea, and total protein, due to RIF and INH treatment, were significantly decreased. The histopathological study further confirmed the biochemical results.
Conclusion: Results of the present study indicated that turmeric has hepatoprotective and renoprotective action against RIF- and INH-induced hepatic and renal injury in rats.
SYNTHESIS, CHARACTERIZATION, AND ANTHELMINTIC ACTIVITY OF NOVEL BENZOTHIAZOLE DERIVATIVES CONTAINING INDOLE MOIETIES
Authors: RAJAREDDY ALETI , MURTHY M SRINIVAS 
Published: October 2019

Objective: The objective of this study was to synthesize and evaluate the anthelmintic activity (AA) of novel benzothiazole derivatives containing indole moieties (BDIM).
Methods: The present works which involve the substituted isatin Schiff bases undergo acetylating and reacting with 2-aminobenzothiazole to give novel BDIM.
Results: All the newly synthesized molecules (5a-5o) were characterized by Fourier-transform infrared spectroscopy, H_nuclear magnetic resonance, and mass spectral analysis along with physical data. The biological potentials of the newly synthesized compounds are evaluated for their AA using an Indian earthworm (Pheretima posthuma), and albendazole was used as standard drug.
Conclusion: The synthesized compound 5f, 5n, and 5o showed good AA, whereas others exhibited significant activities.
IMPACT OF HYDROALCOHOLIC CONCENTRATE OF HEMIDESMUS INDICUS AGAINST 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE PROMPTED PARKINSONISM IN MICE
Authors: V CHITRA , K MANASA , SHARON S EVELYN , CHOUDHURY SHATABDI 
Published: October 2019

Objective: The objective of this study was to evaluate the protective effect of Hemidesmus indicus against 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mice model.
Methods: A total of 18 male Swiss Albino mice were divided into three groups (n=6). Hydroalcoholic concentrate of H. indicus (HAHI) at 200 and 400 mg/kg dosages, 30 min before the MPTP (20 mg/kg, intraperitoneal organization) treatment, was regulated for 7 days, and behavioral assessment was made by rotarod test, grip strength test, locomotor activity, and catatonia behavioral study. Appraisal of cell reinforcement catalysts, such as thiobarbituric acid reactive substance (TBARS), superoxide dismutase, catalepsy, glutathione (GSH) peroxidase, and GSH, were also assessed to screen the neurotoxicity incited by MPTP.
Results: In the present investigation, the neuroprotective impacts of the hydroalcoholic concentrate of H. indicus were assessed, Which is known for its monoamine oxidiase activity, stimulant, anti-convulsant activity and a section of it is used as nerve tonic Frozen at 8 degree C. The cell viability analysis and IC50 determination in SH-SY5Y neuroblastoma cell lines were performed by MTT assay, and neuronal red uptake assay and HAHI were found to have a significant IC50 value in SH-SY5Y cells.
Conclusion: The outcomes demonstrated that HAHI essentially enhanced the behavioral studies, striatal neurotransmitter content, and antioxidant status in a dose-dependent manner lessened TBARS level.
EVALUATION OF THE PROPHYLACTIC ROLE OF INDIAN SHRIMP IN ALUMINUM CHLORIDE-INDUCED ALZHEIMER’S DISEASE ON EXPERIMENTAL RATS
Authors: S RAMACHANDRAN , M SRI RAMYA , U LIZA , PS LAKSHMI PRASANNA , K SARISHMA 
Published: October 2019

Objective: This work was aimed to investigate the prophylactic and therapeutic role of Indian shrimp in aluminum chloride-induced Alzheimer’s disease (AD) in rats.
Methods: The male Wistar rats were selected and divided into six groups. Group I received distilled water, Group II received AlCl3 ( 100 mg/kg, p.o.), Group III received rivastigmine (1 mg/kg, p.o.), Group IV received AlCl3 + shrimp powder (200 mg/kg, p.o), and Group V received AlCl3 + shrimp powder (400 mg/kg, p.o) for 60 days. At the end of the study, various parameters such as behavioral and biochemical investigations were assessed.
Results: The result of the study shows that the shrimp (400 mg/kg) has better effect on the treatment of aluminum chloride-induced AD in rats. It showed a remarkable improvement in the behavioral and biochemical parameters, and the result of histopathology study shows that the hippocampus region of brain tissue recovered as compared with control.
Conclusion: From this study, it is evident that dietary intake of shrimp can help to inhibit oxidative stress produced due to the accumulation of AlCl3 in the brain and used as a prophylactic for AD.
ANTIFUNGAL ACTIVITY OF SEAWEED ULVA LACTUCA L. EXTRACTED CRUDE PROTEIN AGAINST PATHOGENIC FUNGI
Authors: R KRISHNAMOORTHI , SR SIVAKUMAR 
Published: October 2019

Objective: The objective of this study was to evaluate the antifungal activity of seaweed extracted protein against the pathogenic fungi.
Methods: Antifungal activity of seaweed Ulva lactuca L. extracted protein was determined against pathogenic fungi such as Alternaria solani, Aspergillus clavatus, Aspergillus niger, Aspergillus flavus, and Fusarium oxysporum by disk diffusion Method. Then, the potentially active protein was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and circular dichroism (CD) spectroscopy.
Results: Seaweeds extracted protein checked for the antifungal activity against A. clavatus, A. solani, and A. flavus better activity compared to standard amphotericin-B and CD spectroscopy. Analysis of the extracts divulges the presence of entire protein compounds.
Conclusions: This study extracted seaweed protein sufficient of antifungal activity opposed to antifungal pathogens as compared with the standard. This is first report an activity of seaweed extracted protein against the plant and human pathogenic fungus bearing agricultural important.
EFFICACY OF ORAL GLUTATHIONE ADDITION IN LUTEIN SUPPLEMENTATION ON CONTRAST SENSITIVITY IMPROVEMENT IN DRY AGE-RELATED MACULAR DEGENERATION: A RANDOMIZED CONTROLLED TRIAL
Authors: AMIN RAMZI , SAPUTRA EDWIN BAYU 
Published: October 2019

Objective: Age-related macular degeneration (ARMD) is the macular abnormality causing central vision loss in the elderly. One prevention for ARMD is the provision of antioxidants, such as lutein. Reduced form of glutathione (GSH) is a source of cysteine and sulfhydryl, playing a role in detoxification, transport, and metabolic processes. Both are prospected to have a synergistic effect in the prevention of ARMD. This study aimed to determine the efficacy of GSH addition in lutein supplementation to improve the contrast sensitivity of dry type ARMD patients.
Methods: This randomized controlled trial was conducted at an eye hospital and a tertiary general hospital from April 2016 to June 2016. This study involved 22 dry ARMD patients. Subjects were randomized and divided into treatment groups with 20 mg lutein with the addition of 500 mg GSH and 20 mg lutein only, for 30 days. Contrast sensitivity measurement was performed with Lea Numbers® Low Contrast Flip Chart before and after the treatment. Contrast sensitivity was analyzed comparatively with paired t-test.
Results: Contrast sensitivity improvement was observed in both the groups. Contrast sensitivity improvement of the group with additional GSH (3.62±1.44, p<0.05) was a 3-fold of the lutein group only (1.25±0.44, p>0.05). The result was statistically significant for the group treated with GSH addition.
Conclusion: GSH addition in lutein supplementation provoked better contrast sensitivity improvement in dry ARMD patients.
ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF LUDWIGIA PARVIFLORA L. AGAINST STANDARD BACTERIAL STRAINS AND COMPARISON OF ITS ACTIVITY WITH THAT OF STANDARD ANTIBIOTICS
Authors: GEORGE SHIBU , JOY MEVLIN 
Published: October 2019

Objective: The objective of this study was to evaluate the antimicrobial activity of methanolic extract of Ludwigia parviflora L. using standard bacterial strains and compare its activity with that of standard antibiotics.
Methods: The antibacterial activity and antibiotic susceptibility tests were done by disk diffusion method using MTCC bacterial strains.
Results: The study revealed that the methanolic extract of the whole plant of L. parviflora L. was effective to inhibit the growth of Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli. Among the tested strains, S. aureus, P. aeruginosa, K. pneumoniae, and E. coli were more susceptible to the methanolic extract of L. parviflora than the commonly using antibiotic tetracycline 30 mcg. The activity of methanolic extract was also higher than the activity of gentamicin 10 mcg against the P. aeruginosa.
Conclusion: The study concluded that the crude methanolic extract of the whole plant of L. parviflora L. is a good source for antibacterial agent against S. aureus, P. aeruginosa, K. pneumoniae, and E. coli. Hence, this plant can be used as a natural alternative to the common antibiotics such as gentamicin and tetracycline against common bacterial infections after validating its pharmacological and toxicological activities.