bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ikaite nucleation at 35 °C challenges the use of glendonite as a paleotemperature indicator
Authors: Tollefsen Elin , Balic-Zunic Tonci , Mörth Carl-Magnus , Brüchert Volker , Lee Cheng Choo , Skelton Alasdair 
Published: May 2020

AbstractGlendonites have been found worldwide in marine sediments from the Neoproterozoic Era to the Quaternary Period. The precursor of glendonite, ikaite (CaCO3 · 6H2O), is metastable and has only been observed in nature at temperatures <7 °C. Therefore, glendonites in the sedimentary record are commonly used as paleotemperature indicators. However, several laboratory experiments have shown that the mineral can nucleate at temperatures>7 °C. Here we investigate the nucleation range for ikaite as a function of temperature and pH. We found that ikaite precipitated at temperatures of at least 35 °C at pH 9.3 −10.3 from a mixture of natural seawater and sodium carbonate rich solution. At pH 9.3, we observed pseudomorphic replacement of ikaite by porous calcite during the duration of the experiment (c. 5 hours). These results imply that ikaite can form at relatively high temperatures but will then be rapidly replaced by a calcite pseudomorph. This finding challenges the use of glendonites as paleotemperature indicators.
Surgery for Radiologically Normal-Appearing Temporal Lobe Epilepsy in a Centre with Limited Resources
Authors: Arifin Muhamad Thohar , Bakhtiar Yuriz , Andar Erie B. P. S. , Kurnia B. Happy , Priambada Dody , Risdianto Ajid , Kusnarto Gunadi , Tsaniadi Krisna , Bunyamin Jacob , Hanaya Ryosuke , Arita Kazunori , Bintoro Aris Catur , Iida Koji , Kurisu Kaoru , Askoro Rofat , Briliantika Surya P. , Muttaqin Zainal 
Published: May 2020

AbstractApproximately 26–30% of temporal lobe epilepsy (TLE) cases display a normal-appearing magnetic resonance image (MRI) leading to difficulty in determining the epileptogenic focus. This causes challenges in surgical management, especially in countries with limited resources. The medical records of 154 patients with normal-appearing MRI TLE who underwent epilepsy surgery between July 1999 and July 2019 in our epilepsy centre in Indonesia were examined. The primary outcome was the Engel classification of seizures. Anterior temporal lobectomy was performed in 85.1% of the 154 patients, followed by selective amygdalo-hippocampectomy and resection surgery. Of 82 patients (53.2%), Engel Class I result was reported in 69.5% and Class II in 25.6%. The median seizure-free period was 13 (95% CI,12.550–13.450) years, while the seizure-free rate at 5 and 12 years follow-up was 96.3% and 69.0%, respectively. Patients with a sensory aura had better seizure-free outcome 15 (11.575–18.425) years. Anterior temporal lobectomy and selective amygdala-hippocampectomy gave the same favourable outcome. Despite the challenges of surgical procedures for normal MRI TLE, our outcome has been favourable. This study suggests that epilepsy surgery in normal MRI TLE can be performed in centres with limited resources.
Odor Mixtures in Identification Testing Using Sniffin’ Sticks: The SSomix Test
Authors: Liu David Tianxiang , Besser Gerold , Lang Miriam , Sharma Gunjan , Pablik Eleonore , Renner Bertold , Mueller Christian Albert 
Published: May 2020

AbstractClinical tests assessing olfactory performance have become indispensable for diagnosing olfactory dysfunction. As time and personnel resources are limited, it would be advantageous to have shorter protocols focusing on singular aspects of olfactory performance, such as odor identification. However, such a unidimensional approach is often inconclusive and needs further tests (and tools). Hence, new testing methods with high levels of sensitivity, specificity, and reproducibility are required for clinical practice. Here, we developed a Sniffin’ Sticks odor mixture identification test method (SSomix), with emphasis on resource efficiency and simplicity of administration. SSomix consists of mixtures of two and three odors applied onto a piece of paper using 11 out of 16 items from the original Sniffin’ Sticks identification test kit. A total of 66 healthy subjects and 22 patients with olfactory dysfunction were included in the study. SSomix showed good to excellent test-retest reliability and validity. The area under the receiver operating characteristics curves indicated good diagnostic accuracy in identifying patients with reduced and severely impaired olfactory function. SSomix was a suitable downsizing of the original kit, especially regarding resource efficiency.
The value of L3 skeletal muscle index in evaluating preoperative nutritional risk and long-term prognosis in colorectal cancer patients
Authors: Wang Shengqiang , Xie Hailun , Gong Yizhen , Kuang Jiaan , Yan Ling , Ruan Guotian , Gao Feng , Gan Jialiang 
Published: May 2020

AbstractL3 skeletal muscle index (L3SMI) was reportedly related to postoperative outcomes. We aimed to investigate the value of L3SMI in evaluating preoperative nutritional risk and long-term prognosis in colorectal cancer (CRC) patients. We retrospectively enrolled 400 CRC patients who underwent surgery from January 2012 to December 2014. The L3SMI was calculated by preoperative computed tomography (CT) and classified into two groups by gender quartile method. We found that the CT diagnostic criteria of sarcopenia in South China population was: male ≤38.89cm2/m2, female ≤33.28cm2/m2. Multivariate logistic regression analysis showed that low L3SMI was an independent risk factor for preoperative nutritional risk (p < 0.001). Kaplan-Meier survival curves showed that low status group had significantly lower disease-free survival (p = 0.004) and overall survival (p = 0.001), especially in TNM II stage. Multivariate Cox regression analysis revealed preoperative low L3SMI adversely affected disease-free survival (p < 0.001, HR 1.894 (95% CI: 1.330–2.698)), and overall survival (p < 0.001, HR 2.030 (95% CI: 1.420–2.902)). In conclusion, L3SMI is a useful supplement for screening preoperative nutritional risk and diagnosing sarcopenia, and a potential clinical indicator that can be used to predict the prognosis of CRC patients, especially TNM stage II patients.
Increasing carbohydrate oxidation improves contractile reserves and prevents hypertrophy in porcine right heart failure
Authors: Bøgh Nikolaj , Hansen Esben S. S. , Omann Camilla , Lindhardt Jakob , Nielsen Per M. , Stephenson Robert S. , Laustsen Christoffer , Hjortdal Vibeke E. , Agger Peter 
Published: May 2020

AbstractIn heart failure, myocardial overload causes vast metabolic changes that impair cardiac energy production and contribute to deterioration of contractile function. However, metabolic therapy is not used in heart failure care. We aimed to investigate the interplay between cardiac function and myocardial carbohydrate metabolism in a large animal heart failure model. Using magnetic resonance spectroscopy with hyperpolarized pyruvate and magnetic resonance imaging at rest and during pharmacological stress, we investigated the in-vivo cardiac pyruvate metabolism and contractility in a porcine model of chronic pulmonary insufficiency causing right ventricular volume overload. To assess if increasing the carbohydrate metabolic reserve improves the contractile reserve, a group of animals were fed dichloroacetate, an activator of pyruvate oxidation. Volume overload caused heart failure with decreased pyruvate dehydrogenase flux and poor ejection fraction reserve. The animals treated with dichloroacetate had a larger contractile response to dobutamine stress than non-treated animals. Further, dichloroacetate prevented myocardial hypertrophy. The in-vivo metabolic data were validated by mitochondrial respirometry, enzyme activity assays and gene expression analyses. Our results show that pyruvate dehydrogenase kinase inhibition improves the contractile reserve and decreases hypertrophy by augmenting carbohydrate metabolism in porcine heart failure. The approach is promising for metabolic heart failure therapy.
Bragg-Berry flat reflectors for transparent computer-generated holograms and waveguide holography with visible color playback capability
Authors: Cho Seong Yong , Ono Masaru , Yoshida Hiroyuki , Ozaki Masanori 
Published: May 2020

AbstractVarious approaches are being pursued to realize compact optical elements with the ability to manipulate light, but it is difficult to simultaneously achieve high reflectivity and the ability to see through the element. Here, we present a reflective computer-generated hologram that is completely transparent in the visible, based on the Berry (geometric) phase in a self-organizing Bragg reflector. The Bragg reflector has a helical dielectric tensor distribution with the phase information imprinted in the distribution of the optic axis on the substrate. The structure possesses only a single Fourier component and high-order reflections are suppressed; thus, the device appears completely transparent by setting the main reflection band outside the visible range for all angles of incidence accessible by ambient light. On the other hand, the encoded phase information can be played back using visible light by increasing the accessible incidence angle, which we demonstrate experimentally by (i) attaching a coupling prism, and (ii) integrating the device in a waveguide. Bragg-Berry reflectors thus enable a new route to realize advanced optical elements with no apparent reflection in the visible region.
Management of Atrio-esophageal Fistula Induced by Radiofrequency Catheter Ablation in Atrial Fibrillation Patients: a Case Series
Authors: Kim Yun Gi , Shim Jaemin , Lee Kwang-No , Lim Ju Yong , Chung Jae Ho , Jung Jae Seung , Choi Jong-Il , Lee Sung Ho , Son Ho Sung , Kim Young-Hoon 
Published: May 2020

AbstractAtrio-esophageal fistula (AEF) is one of the most devastating complication of radiofrequency catheter ablation (RFCA) of atrial fibrillation (AF) and surgical repair is strongly recommended. However, optimal surgical approach remains to be elucidated. We retrospectively reviewed AEF cases that occurred after RFCA in a single center and evaluated the clinical results of different surgical approach. Surgical or endoscopic repair was attempted in five AF patients who underwent RFCA. Atrio-esophageal fistula and mediastinal infection was not controlled in the patient who underwent endoscopic repair eventually died. Lethal cerebral air embolism occurred two days after surgery in a patient who underwent esophageal repair only. Primary surgical repair of both the left atrium (LA) and esophagus was performed in the remaining three patients. Among these three patients, two underwent external LA repair and the remaining had internal LA repair via open-heart surgery. External repair of the LA was unsuccessful and one patient dies and another had to undergo second operation with internal repair of the LA. The patient who underwent internal LA repair during the first operation survived without additional surgery. Furthermore, we applied veno-arterial extracorporeal membrane oxygenation (VA-ECMO) with artificial induction of ventricular fibrillation in this patient to prevent air and septic embolism and she had no neurologic sequelae. In summary, surgical correction can be considered preferentially to correct AEF. Open-heart surgical repair of LA from the internal side seems to be an acceptable surgical method. Application of VA-ECMO with artificial induction of ventricular fibrillation might be effective to prevent air and septic embolism.
Seasonal concentration distribution of PM1.0 and PM2.5 and a risk assessment of bound trace metals in Harbin, China: Effect of the species distribution of heavy metals and heat supply
Authors: Wang Kun , Wang Weiye , Li Lili , Li Jianju , Wei Liangliang , Chi Wanqiu , Hong Lijing , Zhao Qingliang , Jiang Junqiu 
Published: May 2020

AbstractTo clarify the potential carcinogenic/noncarcinogenic risk posed by particulate matter (PM) in Harbin, a city in China with the typical heat supply, the concentrations of PM1.0 and PM2.5 were analyzed from Nov. 2014 to Nov. 2015, and the compositions of heavy metals and water-soluble ions (WSIs) were determined. The continuous heat supply from October to April led to serious air pollution in Harbin, thus leading to a significant increase in particle numbers (especially for PM1.0). Specifically, coal combustion under heat supply conditions led to significant emissions of PM1.0 and PM2.5, especially heavy metals and secondary atmospheric pollutants, including SO42−, NO3−, and NH4+. Natural occurrences such as dust storms in April and May, as well as straw combustion in October, also contributed to the increase in WSIs and heavy metals. The exposure risk assessment results demonstrated that Zn was the main contributor to the average daily dose through ingestion and inhalation, ADDIng and ADDinh, respectively, among the 8 heavy metals, accounting for 51.7–52.5% of the ADDIng values and 52.5% of the ADDinh values. The contribution of Zn was followed by those of Pb, Cr, Cu and Mn, while those of Ni, Cd, and Co were quite low (<2.2%).
Impact of implementing a non-restrictive antibiotic stewardship program in an emergency department: a four-year quasi-experimental prospective study
Authors: Savoldi Alessia , Foschi Federico , Kreth Florian , Gladstone Beryl Primrose , Carrara Elena , Eisenbeis Simone , Buhl Michael , Marasca Giuseppe , Bovo Chiara , Malek Nisar Peter , Tacconelli Evelina 
Published: May 2020

AbstractAntibiotic resistance is increasing worldwide. The implementation of antibiotic stewardship programmes (ASPs) is of utmost importance to optimize antibiotic use in order to prevent resistance development without harming patients. The emergency department (ED), cornerstone between hospital and community, represents a crucial setting for addressing ASP implementation; however, evidence data on ASP in ED are poor. In this study, a 4-year, non-restrictive, multi-faceted ASP was implemented in a general ED with the aim to evaluate its impact on antibiotic use and costs. Secondly, the study focused on assessing the impact on length of hospital stay (LOS), Clostridioides difficile infection (CDI) incidence rate, and mortality in the patients’ group admitted from ED to medical wards. The ASP implementation was associated with a reduction of antibiotic use and costs. A mild but sustained LOS decrease in all medical wards and a significant downward trend of CDI incidence rate were observed, while mortality did not significantly change. In conclusion, the implementation of our ED-based ASP has demonstrated to be feasible and safe and might clinically benefit the hospital admitted patients’ group. Further research is needed to identify the most suitable ASP design for ED and the key outcome measures to reliably assess its effectiveness.
A swift technique to hydrophobize graphene and increase its mechanical stability and charge carrier density
Authors: Madauß Lukas , Pollmann Erik , Foller Tobias , Schumacher Jens , Hagemann Ulrich , Heckhoff Tobias , Herder Matthias , Skopinski Lucia , Breuer Lars , Hierzenberger Anke , Wittmar Alexandra , Lebius Henning , Benyagoub Abdenacer , Ulbricht Mathias , Joshi Rakesh , Schleberger Marika 
Published: May 2020

AbstractDespite the improvement of the quality of CVD grown single-layer graphene on copper substrates, transferring the two-dimensional layer without introducing any unintentional defects still poses a challenge. While many approaches focus on optimizing the transfer itself or on necessary post-transfer cleaning steps, we have focused on developing a pre-treatment of the monolayer graphene on copper to improve the quality and reproducibility of the transfer process. By pressing an ethylene-vinyl acetate copolymer foil onto the monolayer graphene on copper using a commercially available vacuum bag sealer graphene is stabilized by the attachment of functional carbon groups. As a result, we are able to transfer graphene without the need of any supporting layer in an all-H2O wet-chemical transfer step. Despite the general belief that the crumbling of graphene without a support layer in a H2O environment is caused due to differences in surface energy, we will show that this assumption is false and that this behavior is caused rather by the polar interactions between graphene and water. Suppressing these interactions protects graphene from ripping and results in extremely clean, highly crystalline graphene with a coverage close to 100%.
Macroscale and microcircuit dissociation of focal and generalized human epilepsies
Authors: Weng Yifei , Larivière Sara , Caciagli Lorenzo , Vos de Wael Reinder , Rodríguez-Cruces Raúl , Royer Jessica , Xu Qiang , Bernasconi Neda , Bernasconi Andrea , Thomas Yeo B. T. , Lu Guangming , Zhang Zhiqiang , Bernhardt Boris C. 
Published: May 2020

AbstractThalamo-cortical pathology plays key roles in both generalized and focal epilepsies, but there is little work directly comparing these syndromes at the level of whole-brain mechanisms. Using multimodal imaging, connectomics, and computational simulations, we examined thalamo-cortical and cortico-cortical signatures and underlying microcircuits in 96 genetic generalized (GE) and 107 temporal lobe epilepsy (TLE) patients, along with 65 healthy controls. Structural and functional network profiling highlighted extensive atrophy, microstructural disruptions and decreased thalamo-cortical connectivity in TLE, while GE showed only subtle structural anomalies paralleled by enhanced thalamo-cortical connectivity. Connectome-informed biophysical simulations indicated modest increases in subcortical drive contributing to cortical dynamics in GE, while TLE presented with reduced subcortical drive and imbalanced excitation–inhibition within limbic and somatomotor microcircuits. Multiple sensitivity analyses supported robustness. Our multiscale analyses differentiate human focal and generalized epilepsy at the systems-level, showing paradoxically more severe microcircuit and macroscale imbalances in the former.
Polyoxometalates function as indirect activators of a G protein-coupled receptor
Authors: Althumairy Duaa , Postal Kahoana , Barisas B George , Nunes Giovana G , Roess Deborah A , Crans Debbie C 
Published: May 2020

Abstract
The luteinizing hormone receptor (LHR), a G protein-coupled receptor (GPCRs), can initiate signaling in the presence of some vanadium-containing compounds as a result of vanadium compound interactions with the membrane lipids and/or the cell membrane lipid interface. The ability of LHR expressed in CHO cells to initiate signaling in the presence of highly charged and water-soluble polyoxovanadates (POV) including Na3[H3V10O28] (V10) and two mixed-valence heteropolyoxovanadates, K(NH4)4[H6V14O38(PO4)]·11H2O (V14) and [(CH3)4N]6[V15O36(Cl)] (V15), was investigated here. Interactions of the vanadium compounds with CHO cells decreased the packing of membrane lipids, drove aggregation of LHR and increased signal transduction by LHR. Cell responses were comparable to, or in the case of V14 and V15, greater than those seen for cells treated with human chorionic gonadotropin (hCG), a naturally-occurring LHR ligand produced in early pregnancy in humans. POV effects were observed for CHO cells where LHR was expressed at 10 000 or 32 000 LHR per cell but not when LHR was overexpressed with receptor numbers &gt;100 000 LHR per cell. To determine which POV species were present in the cell medium during cell studies, the speciation of vanadate (V1), V10, V14 or V15 in cell medium was monitored using 51V NMR and EPR spectroscopies. We found that all the POVs initiated signaling, but V15 and V10 had the greatest effects on cell function, while V1 was significantly less active. However, because of the complex nature of vanadium compounds speciation, the effects on cell function may be due to vanadium species formed in the cell medium over time.
Deregulation of miRNAs-cMYC circuits is a key event in refractory celiac disease type-2 lymphomagenesis
Authors: Vaira Valentina , Gaudioso Gabriella , Laginestra Maria Antonella , Terrasi Andrea , Agostinelli Claudio , Bosari Silvano , Di Sabatino Antonio , Vanoli Alessandro , Paulli Marco , Ferrero Stefano , Roncoroni Leda , Lombardo Vincenza , Perera Liyanage P. , Fabris Sonia , Vecchi Maurizio , Pileri Stefano , Elli Luca 
Published: May 2020

Abstract
A percentage of celiac disease (CD) patients develop refractory type-2 disease (RCD2), a condition associated with increased risk of enteropathy-associated T-cell-lymphoma (EATL) and without therapeutic option. Therefore, we profiled the miRNome in series of peripheral T-cell lymphomas (PTCLs), CD, RCD1 or 2 and in the murine interleukin-15 (IL15)-transgenic (TG) model of RCD. The transcriptome was analyzed in 18 intestinal T-cell lymphomas (ITLs). Bioinformatics pipelines provided significant microRNA (miRNA) lists and predicted targets that were confirmed in a second set of patients.
Our data show that ITLs have a unique miRNA profile with respect to other PTCLs. The c-MYC regulated miR-17/92 cluster distinguishes monomorphic epitheliotropic ITL (MEITL) from EATL and prognosticates EATL outcome. These miRNAs are decreased in IL15-TG mice upon Janus kinase (JAK) inhibition. The random forest algorithm identified a signature of 38 classifier miRNAs, among which, the miR-200 and miR-192/215 families were progressively lost in RCD2 and ITL-CD, whereas miR-17/92 and C19MC miRNAs were up-regulated. Accordingly, SMAD3, MDM2, c-Myc and activated-STAT3 were increased in RCD2 and EATL tissues while JAK inhibition in IL15-TG mice restored their levels to baseline.
Our data suggest that miRNAs circuit supports activation of STAT3 and c-Myc oncogenic signaling in RCD2, thus contributing to lymphomagenesis. This novel understanding might pave the way to personalized medicine approaches for RCD and EATL.
Capture of satellites during planetary encounters
Authors: Li Daohai , Johansen Anders , Mustill Alexander J. , Davies Melvyn B. , Christou Apostolos A. 
Published: May 2020

Context.Single-binary scattering may lead to an exchange where the single object captures a component of the binary, forming a new binary. This has been well studied in encounters between a star–planet pair and a single star.Aims.Here we explore the application of the exchange mechanism to a planet–satellite pair and another planet in the gravitational potential of a central star. As a case study, we focus on encounters between a satellite-bearing object and Neptune. We investigate whether Neptune can capture satellites from that object and if the captured satellites have orbits analogous to the Neptunian moons Triton and Nereid.Methods.UsingN-body simulations, we study the capture probability at different encounter distances. Post-capture, we use a simple analytical argument to estimate how the captured orbits evolve under collisional and tidal effects.Results.We find that the average capture probability reaches ~10% if Neptune penetrates the donor planet’s satellite system. Most moons grabbed by Neptune acquire highly eccentric orbits. Post-capture, around half of those captured, especially those on tight orbits, can be circularised, either by tides only or by collisions+tides, turning into Triton-like objects. Captures further out, on the other hand, stay on wide and eccentric orbits like that of Nereid. Both moon types can be captured in the same encounter and they have wide distributions in orbital inclination. Therefore, Triton naturally has a ~50% chance of being retrograde.Conclusions.A similar process potentially applies to an exoplanetary system, and our model predicts that exomoons can jump from one planet to another during planetary scattering. Specifically, there should be two distinct populations of captured moons: one on close-in circular orbits and the other on far-out eccentric orbits. The two populations may have highly inclined prograde or retrograde orbits.