bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic diversity and core subset selection inex situseed collections of the banana crop wild relativeMusa balbisiana
Authors: Bawin Yves , Panis Bart , Vanden Abeele Samuel , Li Zhiying , Sardos Julie , Paofa Janet , Ge Xue-Jun , Mertens Arne , Honnay Olivier , Janssens Steven B. 
Published: November 2019

AbstractCrop wild relatives (CWRs) play a key role in crop breeding by providing beneficial trait characteristics for improvement of related crops. CWRs are more efficiently used in breeding if the plant material is genetically characterized, but the diversity in CWR genetic resources has often poorly been assessed. Seven seed collections ofMusa balbisiana, an important CWR of dessert and cooking bananas, originating from three natural populations, two feral populations and twoex situfield collections were retrieved and their genetic diversity was quantified using 18 microsatellite markers to select core subsets that conserve the maximum genetic diversity. The highest genetic diversity was observed in the seed collections from natural populations of Yunnan, a region that is part ofM. balbisiana's centre of origin. The seeds from theex situfield collections were less genetically diverse, but contained unique variation with regards to the diversity in all seed collections. Seeds from feral populations displayed low genetic diversity. Core subsets that maximized genetic distance incorporated almost no seeds from theex situfield collections. In contrast, core subsets that maximized allelic richness contained seeds from theex situfield collections. We recommend the conservation and additional collection of seeds from natural populations, preferentially originating from the species' region of origin, and from multiple individuals in one population. We also suggest that the number of seeds used forex situseed bank regeneration must be much higher for the seed collections from natural populations.
Correlation of Remotely Sensed Surface Reflectance With Forcing Variables in Six Different Estuaries
Authors: Tao Jing , Hill Paul S. 
Published: November 2019

AbstractThis study examines the links between estuarine dynamics and longitudinal distributions of remotely sensed reflectance in estuaries. Reflectance at 655 nm from Landsat‐8 correlates with in situ measurements of surface turbidity. Images collected from 2013 to 2018 are used to investigate the spatial and temporal characteristics of reflectance distribution in six selected estuaries with different dynamics. The results show that the maximum magnitude (C
) and location (X
) of the reflectance are functions of two major forcing variables, the freshwater discharge and tidal stage. Salt wedge estuaries (i.e., the Fraser River, Connecticut River, and Columbia River estuary) are affected strongly by river discharge and are relatively less affected by tidal forcing. In salt wedge estuaries, the C
values along the river channel are correlated with river discharge, while the X
values generally are not. In partially mixed to strain‐induced periodic stratification (SIPS) estuaries (i.e., Delaware River) and SIPS‐to‐well‐mixed estuaries (i.e., Gironde estuary), the X
values are correlated with river discharge, but the C
values are not. SIPS‐to‐well‐mixed estuaries (i.e., Gironde estuary) and highly time‐dependent salt wedge to well‐mixed estuaries (i.e., Merrimack River estuary) are affected by tidal forcing. In these estuaries, the C
values are correlated with tidal stage. The C
values tend to be lower around high tide than low tide. Overall, the results demonstrate that remote sensing observations of ocean color can be utilized to infer subsurface estuarine processes. Satellite ocean color is a potential tool to monitor river discharge and to classify estuaries.
The Challenge Posed by Space Weather to High‐Voltage Electricity Flows: Evidence From Ontario, Canada, and New York State, USA
Authors: Forbes Kevin F. , St. Cyr O. C. 
Published: November 2019

AbstractIn this paper, we present evidence that geomagnetic activity has the potential to disrupt the electricity flows between Ontario, Canada, and New York State, USA. This conclusion is based on a modeling framework that makes use of weather data, electricity load data, measures of transmission “network effects,” proxies for geomagnetically induced currents and the expected level of power grid conditions. The model is estimated using hourly data throughout 1 May 2002 through 31 October 2003. The model is evaluated using out‐of‐sample hourly data over the period of 1 November to 9 December 2003. The out‐of‐sample predictions are more accurate when the forecasting equation reflects the estimated contribution of geomagnetic activity. The structural modeling results for the sample period indicate that the peak predicted effect of geomagnetic activity on the electricity flow was about 1,604 MWh in absolute value when the geomagnetically induced current proxy achieved a value of 363.2 nT/min. The analysis also indicates that the level of the geomagnetically induced electricity flow is highly dependent on ambient temperature and expected system conditions at the time of the geomagnetic storm. Moreover, geomagnetic activity is an important driver of the volatility in the electricity flows. The overall findings indicate that the scope of the challenge posed by space weather to the operations of the electric power system is likely understated.
Integration of Ki-67 index into AJCC 2018 staging provides additional prognostic information in breast tumours candidate for genomic profiling
Authors: Vissio Elena , Metovic Jasna , Osella-Abate Simona , Bertero Luca , Migliaretti Giuseppe , Borella Fulvio , Benedetto Chiara , Sapino Anna , Cassoni Paola , Castellano Isabella 
Published: November 2019

Abstract
Background
The Eighth edition of the American Joint Committee on Cancer (AJCC) staging system (2018) for breast cancer (BC) introduced the prognostic stage. Moreover, multigene assessment has been indicated to tailor staging in T1/T2/N0, ER-positive/HER2-negative BC. However, many National Health Systems do not provide reimbursement for routine testing. The aim of this study was to assess whether Ki67 proliferation index is prognostically relevant for patients’ candidacy for molecular testing.

Methods
A retrospective series of 686 ER+/HER2− BC were reclassified using AJCC 2018, and in the group of 521 patients for which AJCC 2018 recommends molecular evaluation, we assessed the prognostic efficacy of a prognostic stage enriched by Ki67 (Ki67-PS), considering Ki67 <20% an alternative to recurrence score <11 provided by Oncotype DX.

Results
We found that a group of BCs (35.6%, 58/163) assigned to IB stage by prognostic score were down classified to IA with Ki67-PS. The outcome of these 58 cases overlapped with that of lesions classified as stage IA using prognostic stage, showing a significantly better prognosis compared to IB tumours (HR = 2.79, p = 0.003).

Conclusions
These data suggest that Ki67 may be a reliable marker to enrich the 2018 AJCC prognostic score in BC patients’ candidacy for genomic profiling.
Acoustic enrichment can enhance fish community development on degraded coral reef habitat
Authors: Gordon Timothy A. C. , Radford Andrew N. , Davidson Isla K. , Barnes Kasey , McCloskey Kieran , Nedelec Sophie L. , Meekan Mark G. , McCormick Mark I. , Simpson Stephen D. 
Published: November 2019

AbstractCoral reefs worldwide are increasingly damaged by anthropogenic stressors, necessitating novel approaches for their management. Maintaining healthy fish communities counteracts reef degradation, but degraded reefs smell and sound less attractive to settlement-stage fishes than their healthy states. Here, using a six-week field experiment, we demonstrate that playback of healthy reef sound can increase fish settlement and retention to degraded habitat. We compare fish community development on acoustically enriched coral-rubble patch reefs with acoustically unmanipulated controls. Acoustic enrichment enhances fish community development across all major trophic guilds, with a doubling in overall abundance and 50% greater species richness. If combined with active habitat restoration and effective conservation measures, rebuilding fish communities in this manner might accelerate ecosystem recovery at multiple spatial and temporal scales. Acoustic enrichment shows promise as a novel tool for the active management of degraded coral reefs.
Chemical unfolding of protein domains induces shape change in programmed protein hydrogels
Authors: Khoury Luai R. , Popa Ionel 
Published: November 2019

AbstractProgrammable behavior combined with tailored stiffness and tunable biomechanical response are key requirements for developing successful materials. However, these properties are still an elusive goal for protein-based biomaterials. Here, we use protein-polymer interactions to manipulate the stiffness of protein-based hydrogels made from bovine serum albumin (BSA) by using polyelectrolytes such as polyethyleneimine (PEI) and poly-L-lysine (PLL) at various concentrations. This approach confers protein-hydrogels with tunable wide-range stiffness, from ~10–64 kPa, without affecting the protein mechanics and nanostructure. We use the 6-fold increase in stiffness induced by PEI to program BSA hydrogels in various shapes. By utilizing the characteristic protein unfolding we can induce reversible shape-memory behavior of these composite materials using chemical denaturing solutions. The approach demonstrated here, based on protein engineering and polymer reinforcing, may enable the development and investigation of smart biomaterials and extend protein hydrogel capabilities beyond their conventional applications.
Homogeneous cobalt-catalyzed reductive amination for synthesis of functionalized primary amines
Authors: Murugesan Kathiravan , Wei Zhihong , Chandrashekhar Vishwas G. , Neumann Helfried , Spannenberg Anke , Jiao Haijun , Beller Matthias , Jagadeesh Rajenahally V. 
Published: November 2019

AbstractThe development of earth abundant 3d metal-based catalysts continues to be an important goal of chemical research. In particular, the design of base metal complexes for reductive amination to produce primary amines remains as challenging. Here, we report the combination of cobalt and linear-triphos (bis(2-diphenylphosphinoethyl)phenylphosphine) as the molecularly-defined non-noble metal catalyst for the synthesis of linear and branched benzylic, heterocyclic and aliphatic primary amines from carbonyl compounds, gaseous ammonia and hydrogen in good to excellent yields. Noteworthy, this cobalt catalyst exhibits high selectivity and as a result the -NH2 moiety is introduced in functionalized and structurally diverse molecules. An inner-sphere mechanism on the basis of the mono-cationic [triphos-CoH]+ complex as active catalyst is proposed and supported with density functional theory computation on the doublet state potential free energy surface and H2 metathesis is found as the rate-determining step.
Imaging and writing magnetic domains in the non-collinear antiferromagnet Mn3Sn
Authors: Reichlova Helena , Janda Tomas , Godinho Joao , Markou Anastasios , Kriegner Dominik , Schlitz Richard , Zelezny Jakub , Soban Zbynek , Bejarano Mauricio , Schultheiss Helmut , Nemec Petr , Jungwirth Tomas , Felser Claudia , Wunderlich Joerg , Goennenwein Sebastian T. B. 
Published: November 2019

AbstractNon-collinear antiferromagnets are revealing many unexpected phenomena and they became crucial for the field of antiferromagnetic spintronics. To visualize and prepare a well-defined domain structure is of key importance. The spatial magnetic contrast, however, remains extraordinarily difficult to be observed experimentally. Here, we demonstrate a magnetic imaging technique based on a laser induced local thermal gradient combined with detection of the anomalous Nernst effect. We employ this method in one the most actively studied representatives of this class of materials—Mn3Sn. We demonstrate that the observed contrast is of magnetic origin. We further show an algorithm to prepare a well-defined domain pattern at room temperature based on heat assisted recording principle. Our study opens up a prospect to study spintronics phenomena in non-collinear antiferromagnets with spatial resolution.
Hydrogen bond guidance and aromatic stacking drive liquid-liquid phase separation of intrinsically disordered histidine-rich peptides
Authors: Gabryelczyk Bartosz , Cai Hao , Shi Xiangyan , Sun Yue , Swinkels Piet J. M. , Salentinig Stefan , Pervushin Konstantin , Miserez Ali 
Published: November 2019

AbstractLiquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs) is involved in both intracellular membraneless organelles and extracellular tissues. Despite growing understanding of LLPS, molecular-level mechanisms behind this process are still not fully established. Here, we use histidine-rich squid beak proteins (HBPs) as model IDPs to shed light on molecular interactions governing LLPS. We show that LLPS of HBPs is mediated though specific modular repeats. The morphology of separated phases (liquid-like versus hydrogels) correlates with the repeats’ hydrophobicity. Solution-state NMR indicates that LLPS is a multistep process initiated by deprotonation of histidine residues, followed by transient hydrogen bonding with tyrosine, and eventually by hydrophobic interactions. The microdroplets are stabilized by aromatic clustering of tyrosine residues exhibiting restricted molecular mobility in the nano-to-microsecond timescale according to solid-state NMR experiments. Our findings provide guidelines to rationally design pH-responsive peptides with LLPS ability for various applications, including bioinspired protocells and smart drug-delivery systems.
RETRACTED ARTICLE: Novel Therapeutic Approach Using Drug-loaded Adipose-derived Stem Cells for Pancreatic Cancer
Authors: Aoki Masahiko , Kakimoto Kazuki , Goto Masahiro , Higuchi Kazuhide 
Published: November 2019

AbstractWe developed anticancer drug-conjugated biodegradable polymer-nanoparticle-loaded adipose-derived stem cells (AdSCs) as a tool for biodrug delivery systems for cancer therapy. Pirarubicin was conjugated in polylactic/glycolic acid (PLGA) followed by formation of nanoparticles (NPs), which were loaded with human AdSCs and cocultured. The pirarubicin-conjugated PLGA NP-loaded AdSCs (PirNP-AdSCs) were overall viable within 48 h and exhibited significantly enhanced migration activity. We confirmed that pirarubicin was gradually released into the culture medium from PirNP-AdSCs, and the conditioned medium significantly inhibited the proliferation activity and induced the apoptosis of human pancreatic cancer cells (KP1N). PirNP-AdSCs also significantly induced tumor cell apoptosis in anex vivoculture system with KP1N-derived tumors, and there was increased invasion/migration of PirNP-AdSCs inside the tumor. Finally, we compared the therapeutic efficacy of the PirNP-AdSCs on KP1N-derived tumor growth with that of treatments of AdSCs alone, PirNPs alone or normal saline (control) in immunodeficient mice. Subcutaneous local administration of PirNP-AdSCs significantly inhibited tumor growth, inducing the apoptosis of tumor cells and vasculature compared with the other groups. The present therapeutic strategy might give rise to a novel cancer therapy minimizing the adverse side effects of anticancer drugs in patients who suffer from cancer.
Global optimization of metasurface designs using statistical learning methods
Authors: Elsawy Mahmoud M. R. , Lanteri Stéphane , Duvigneau Régis , Brière Gauthier , Mohamed Mohamed Sabry , Genevet Patrice 
Published: November 2019

AbstractOptimization of the performance of flat optical components, also dubbed metasurfaces, is a crucial step towards their implementation in realistic optical systems. Yet, most of the design techniques, which rely on large parameter search to calculate the optical scattering response of elementary building blocks, do not account for near-field interactions that strongly influence the device performance. In this work, we exploit two advanced optimization techniques based on statistical learning and evolutionary strategies together with a fullwave high order Discontinuous Galerkin Time-Domain (DGTD) solver to optimize phase gradient metasurfaces. We first review the main features of these optimization techniques and then show that they can outperform most of the available designs proposed in the literature. Statistical learning is particularly interesting for optimizing complex problems containing several global minima/maxima. We then demonstrate optimal designs for GaN semiconductor phase gradient metasurfaces operating at visible wavelengths. Our numerical results reveal that rectangular and cylindrical nanopillar arrays can achieve more than respectively 88% and 85% of diffraction efficiency for TM polarization and both TM and TE polarization respectively, using only 150 fullwave simulations. To the best of our knowledge, this is the highest blazed diffraction efficiency reported so far at visible wavelength using such metasurface architectures.
Left Ventricular Deformation in Patients with Connective Tissue Disease: Evaluated by 3.0T Cardiac Magnetic Resonance Tissue Tracking
Authors: Wang Jin , Shi Ke , Xu Hua-yan , Zhao Qin , Liu Xi , Gao Yue , Yu Hong , Guo Ying-kun , Yang Zhi-gang 
Published: November 2019

AbstractThe aim of this study was to assess left ventricular (LV) myocardial strain in patients with connective tissue disease (CTD) and compare LV deformation between subgroups of idiopathic inflammatory myopathy (IIM) and non-IIM. Ninety-eight patients with CTD, comprising 56 with IIM and 42 with non-IIM, and 30 healthy subjects were enrolled and underwent 3.0T cardiac magnetic resonance imaging (MRI) scanning. The LV function and strain parameters were measured and assessed. Our result revealed that CTD patients had preserved LV ejection fraction (60.85%) and had significantly decreased global and regional peak strain (PS) in radial, circumferential, and longitudinal directions (all p < 0.05). IIM patients showed significantly reduced global longitudinal PS (GLPS) and longitudinal PS at apical slice, whereas all strain parameters decreased in non-IIM patients. Except GLPS and longitudinal PS at apical slice, all strain parameters in non-IIM patients were lower than those in IIM patients. By Pearson’s correlation analysis, the LV global radial and circumferential PS were correlated to N-terminal pro-brain natriuretic peptide level and LV ejection fraction in both IIM and non-IIM patients. This study indicated that CTD patients showed abnormal LV deformation despite with preserved LVEF. The impairment of LV deformation differed between IIM and non-IIM patients.
Initial radiographic tracheal ratio in predicting clinical outcomes in croup in children
Authors: Yang Wen-Chieh , Hsu Yu-Lung , Chen Chun-Yu , Peng Yi-Chin , Chen Jun-Nong , Fu Yun-Ching , Chang Yu-Jun , Lee En-Pei , Lin Mao-Jen , Wu Han-Ping 
Published: November 2019

AbstractCroup is the leading infectious disease resulting in pediatric upper airway obstruction. Our purpose is to analyze diverse features of neck radiographs could be seen as an objective tool to predict outcomes in patients with croup. One hundred and ninety-two patients were prospectively recruited in pediatric emergency department with diagnosis of croup. The initial Westley score (WS), presence of steeple sign, extent of narrowing, and narrowing ratio on soft tissue neck radiographs were determined before and after treatments. The extent of frontal narrowing, extent of lateral narrowing, frontal ratio (FR), and lateral ratio (LR) were investigated to predict clinical outcomes in patients with croup. The extent of frontal/lateral narrowing and LR had significant correlation with outpatient status. Almost 71% of patients with FR values below 0.23 stayed in the hospital longer, whereas nearly 98% of patients with FR vales above 0.65 could be discharged. About 85% of patients with LR below 0.45 hospitalized longer. The LR and FR were significantly correlated with the severity and admission rate in croup. The LR > 0.6 and FR > 0.65 may indicate low risk in patients with croup, whereas the FR < 0.23 or LR < 0.45 may indicate the need of stay in hospital for further treatment and monitor.
Combined dietary supplementation of long chain inulin and Lactobacillus acidophilus W37 supports oral vaccination efficacy against Salmonella Typhimurium in piglets
Authors: Lépine Alexia F. P. , Konstanti Prokopis , Borewicz Klaudyna , Resink Jan-Willem , de Wit Nicole J. , Vos Paul de , Smidt Hauke , Mes Jurriaan J. 
Published: November 2019

AbstractRoutine use of antibiotics in livestock animals strongly contributed to the creation of multidrug-resistant Salmonella Typhimurium strains (STM). Vaccination is an alternative to the use of antibiotics but often suffers from low efficacy. The present study investigated whether long-chain inulin (lcITF) and Lactobacillus acidophilus W37 (LaW37) can support vaccination efficacy against STM and if the interventions influence possible gut microbiota changes. Piglets received daily supplementation until sacrifice. Animals were vaccinated on day 25 after birth, one day after weaning, and were challenged with STM on days 52–54. Dietary intervention with lcITF/LaW37 enhanced vaccination efficacy by 2-fold during challenge and resulted in higher relative abundance of Prevotellaceae and lower relative abundance of Lactobacillaceae in faeces. Although strongest microbial effects were observed post STM challenge on day 55, transient effects of the lcITF/LaW37 intervention were also detected on day 10 after birth, and post-weaning on day 30 where increased relative abundance of faecal lactobacilli was correlated with higher faecal consistency. LcITF treatment increased post-weaning feed efficiency and faecal consistency but did not support vaccination efficacy. Vaccination in immune-immature young animals can be enhanced with functional additives which can simultaneously promote health in an ingredient-dependent fashion.
The Usefulness of Lung Ultrasound for the Aetiological Diagnosis of Community-Acquired Pneumonia in Children
Authors: Berce Vojko , Tomazin Maja , Gorenjak Mario , Berce Tadej , Lovrenčič Barbara 
Published: November 2019

AbstractThe aetiology of community-acquired pneumonia (CAP) is not easy to establish. As lung ultrasound (LUS) has already proved to be an excellent diagnostic tool for CAP, we analysed its usefulness for discriminating between the aetiologically different types of CAP in children. We included 147 children hospitalized because of CAP. LUS was performed in all patients at admission, and follow-up LUS was performed in most patients. LUS-detected consolidations in viral CAP were significantly smaller, with a median diameter of 15 mm, compared to 20 mm in atypical bacterial CAP (p = 0.05) and 30 mm in bacterial CAP (p < 0.001). Multiple consolidations were detected in 65.4% of patients with viral CAP and in 17.3% of patients with bacterial CAP (p < 0.001). Bilateral consolidations were also more common in viral CAP than in bacterial CAP (51.9% vs. 8.0%, p < 0.001). At follow-up, a regression of consolidations was observed in 96.6% of patients with bacterial CAP and in 33.3% of patients with viral CAP (p < 0.001). We found LUS to be especially suitable for differentiating bacterial CAP from CAP due to other aetiologies. However, LUS must be interpreted in light of clinical and laboratory findings.