bluuebunny/crossref_metadata_2025_split huggingface dataset:
Survival in patients with HR+/HER2− metastatic breast cancer treated with initial endocrine therapy versus initial chemotherapy. A French population-based study
Authors: Simon Julien , Chaix Marie , Billa Oumar , Kamga Ariane Mamguem , Roignot Patrick , Ladoire Sylvain , Coutant Charles , Arveux Patrick , Quantin Catherine , Dabakuyo-Yonli Tienhan Sandrine 
Published: July 2020

Abstract
Background
According to international guidelines, endocrine therapy (ET) is the preferred option for hormone receptor-positive (HR+) HER2-negative (HER2−) metastatic breast cancer. In spite of clear recommendations, these are not strictly followed in daily practice. The objectives of this study were to investigate the effect of the first anti-metastatic treatment therapy choice on progression-free survival (PFS) and overall survival (OS).

Methods
In this population-based study, we included patients with HR+/HER2− metastatic breast cancer recorded in the Côte d’Or Breast Cancer Registry. Differences in PFS and OS between patients initially treated with chemotherapy (CT) or ET were analysed in Cox proportional hazards models. In a sensitivity analysis, we used a propensity score (PS) to limit the indication bias.

Results
Altogether, 557 cases were included, 280 received initial ET and 277 received initial CT. PFS and OS in patients initially treated with ET was improved significantly when compared to patients with initial CT (respectively, HR = 0.83 (95% CI 0.69–0.99) and HR = 0.71 (95% CI 0.58–0.86)). The results of the sensitivity analysis supported these findings.

Conclusion
This study shows that treating patients with HR+/HER2− metastatic breast cancer with initial ET could provide a survival advantage in comparison with initial CT.
Quantitative Investigation into the influence of intravenous fluids on human immune and cancer cell lines
Authors: Karamahmutoglu Hande , Altay Alara , Vural Sumeyra , Elitas Meltem 
Published: July 2020

AbstractThe effect of intravenous fluids (IVF) has been investigated clinically through the assessment of post-treatment reactions. However, the responses to IVF vary from patient-to-patient. It is important to understand the response of IVF treatment to be able to provide optimal IVF care. Herein, we investigated the impact of commonly used IVFs, Dextrose, NaCl and Ringer on different human cancer (HepG2 (liver hepatocellular carcinoma) and MCF7 (breast adenocarcinoma)) and immune cell lines (U937 (lymphoma) monocyte and macrophages). The effect of IVF exposure on single cells was characterized using hemocytometer, fluorescence microscopy and flow cytometry. Quantitative data on the viability and morphology of the cells were obtained. Our results emphasize that different IVFs demonstrate important differences in how they influence distinct cell lines. Particularly, we observed that the lactated ringer and dextrose solutions altered the viability and nuclear size of cancer and immune cells differently. Our findings present valuable information to the knowledge of cellular-level IVF effects for further investigations in IVF usage on diverse patient populations and support the importance and necessity of developing optimal diluents not only for drug stability but also for patient benefits.
First Reports of Effects of Insulin, Human-like Insulin Receptors and Adapter Proteins in Acanthamoeba castellanii
Authors: Baig Abdul Mannan , Khaleeq Areeba 
Published: July 2020

AbstractThe insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF1-R) play key roles in growth, regulation of nutrient metabolism and carbohydrate homeostasis. Insulin-like molecules in prokaryotes and other early life have been reported. However, an account of metabolic effects of insulin, transcriptomic evidence of expression of glucose transporting channels (GLUT) and homology modelling of IR and IGF1-R like proteins in unicellular life-forms have yet to be established. Acanthamoeba spp. has existed for about 2 billion years and is one of the earliest mitochondriate unicellular eukaryotic cells on Earth. Despite Acanthamoeba spp. being grown in a medium called peptone-yeast-glucose (PYG) for over 50 years, the mechanism and regulation of glucose uptake by IR or IGF1-R molecules in this microbe has not yet been reported. Several methods were utilized to validate the effects of insulin on trophozoites of A. castellanii, including: growth assays with insulin, estimation of glucose and potassium (K+) entry into the cell, and histology showing anabolic effects on proteins. Bioinformatic computational tools and homology modeling demonstrated the involvement of IR like proteins, GLUT, and adapter proteins in mediating the IR cascade. Growth assays showed proliferative effects in a dose range of 2.98–5.97 µmol/mL of insulin. After insulin exposure, A. castellanii trophozoites displayed enhanced Periodic acid-Sciff (PAS) staining. Amino acid sequence similarities and homology modelling revealed ACA1_163470 in Acanthamoeba spp. to be a homolog of human-IR. Acanthamoeba protein ACA1_336150 shares similarities with IGF1-R. Additionally, some proteins like ACA1_060920 have attributes of GLUT like channels on homology modelling and show similarity with human GLUT. Knowledge of IR and insulin effects in Acanthamoeba spp. contributes to its biology and advances current understanding behind the evolution of IR and IGF1-R signalling cascade.
A Preliminary Report on the Largest Ongoing Outbreak of Lead Toxicity in Iran
Authors: Zamani Nasim , Mehrpour Omid , Hassanian-Moghaddam Hossein , Jalali Maryam , Amirabadizadeh Alireza , Samie Saeed , Sabeti Shahram , Kolahi Ali-Asghar 
Published: July 2020

AbstractNo countrywide data exists on the patients’ characteristics of lead exposure in Iran. We aimed to evaluate the demographic characteristics and blood lead level (BLL) of these patients in the country scale during five consecutive years, including the epidemic outbreak year (2016). Between 2014 and 2018, records of all patients who had referred to two reference laboratories in Tehran, Iran, to check BLL were evaluated. Of 58,642 patients, 48,589 were male. Mean age was 44.9 ± 20.7 years. Males had higher BLLs and were significantly older. Median BLL was 16 µg/dL (0.3 to 263 µg/dL). Median BLL was significantly higher in 45- to 60-year-old patients. The highest median BLL was reported in May 2016 confirming our records about the peak of the epidemic. Although the frequency of high BLL declined after 2016, it never returned to the measures before that. Considering the ongoing high prevalence of increased BLLs after 2016 and similar environmental and occupational exposures as before, lead-contaminated opium still seems to persist in the Iranian opium black market. Substitution of this lead-contaminated opium by Opioid Maintenance Therapy (OMT)-prescribed opium tincture is recommended.
Neural feedback strategies to improve grasping coordination in neuromusculoskeletal prostheses
Authors: Mastinu Enzo , Engels Leonard F. , Clemente Francesco , Dione Mariama , Sassu Paolo , Aszmann Oskar , Brånemark Rickard , Håkansson Bo , Controzzi Marco , Wessberg Johan , Cipriani Christian , Ortiz-Catalan Max 
Published: July 2020

AbstractConventional prosthetic arms suffer from poor controllability and lack of sensory feedback. Owing to the absence of tactile sensory information, prosthetic users must rely on incidental visual and auditory cues. In this study, we investigated the effect of providing tactile perception on motor coordination during routine grasping and grasping under uncertainty. Three transhumeral amputees were implanted with an osseointegrated percutaneous implant system for direct skeletal attachment and bidirectional communication with implanted neuromuscular electrodes. This neuromusculoskeletal prosthesis is a novel concept of artificial limb replacement that allows to extract control signals from electrodes implanted on viable muscle tissue, and to stimulate severed afferent nerve fibers to provide somatosensory feedback. Subjects received tactile feedback using three biologically inspired stimulation paradigms while performing a pick and lift test. The grasped object was instrumented to record grasping and lifting forces and its weight was either constant or unexpectedly changed in between trials. The results were also compared to the no-feedback control condition. Our findings confirm, in line with the neuroscientific literature, that somatosensory feedback is necessary for motor coordination during grasping. Our results also indicate that feedback is more relevant under uncertainty, and its effectiveness can be influenced by the selected neuromodulation paradigm and arguably also the prior experience of the prosthesis user.
DNA methylation of the long intergenic noncoding RNA 299 gene in triple-negative breast cancer: results from a prospective study
Authors: Manoochehri Mehdi , Jones Michael , Tomczyk Katarzyna , Fletcher Olivia , Schoemaker Minouk J. , Swerdlow Anthony J. , Borhani Nasim , Hamann Ute 
Published: July 2020

AbstractTriple-negative breast cancer (TNBC) is an aggressive breast cancer subtype associated with a high rate of recurrence and poor prognosis. Recently we identified a hypermethylation in the long noncoding RNA 299 (LINC00299) gene in blood-derived DNA from TNBC patients compared with healthy controls implying that LINC00299 hypermethylation may serve as a circulating biomarker for TNBC. In the present study, we investigated whether LINC00299 methylation is associated with TNBC in a prospective nested breast cancer case–control study within the Generations Study. Methylation at cg06588802 in LINC00299 was measured in 154 TNBC cases and 159 breast cancer-free matched controls using MethyLight droplet digital PCR. To assess the association between methylation level and TNBC risk, logistic regression was used to calculate odd ratios and 95% confidence intervals, adjusted for smoking status. We found no evidence for association between methylation levels and TNBC overall (P = 0.062). Subgroup analysis according to age at diagnosis and age at blood draw revealed increased methylation levels in TNBC cases compared with controls in the young age groups [age 26–52 (P = 0.0025) and age 22–46 (P = 0.001), respectively]. Our results suggest a potential association of LINC00299 hypermethylation with TNBC in young women.
Bayesian method for inferring the impact of geographical distance on intensity of communication
Authors: Ozga Fei , Onnela Jukka-Pekka , DeGruttola Victor 
Published: July 2020

AbstractSpatially-embedded networks represent a large class of real-world networks of great scientific and societal interest. For example, transportation networks (such as railways), communication networks (such as Internet routers), and biological networks (such as fungal foraging networks) are all spatially embedded. Both the density of interactions (presence of edges) and intensity of interactions (edge weights) are typically found to decrease as a function of spatial separation of nodes in these networks. Communication and mobility of groups of individuals have also been shown to decline with their spatial separation, and the so-called gravity model postulates that this decline takes the form of a power-law holding at all distances. There is however some evidence that the rate of decline might change as the distance increases beyond a certain value, called a change point, but there have been few statistically principled methods for determining the existence and location of change points or assessing the change in intensity of interactions associated with them. We introduce such a method within the Bayesian paradigm and apply it to anonymized mobile call detail records (CDRs). Our results are potentially useful in settings where understanding social and spatial mixing of people is important, such as in the design of cluster randomized trials for studying interventions for infectious diseases, but we also anticipate the method to be useful for investigating more generally how distance may affect tie strengths in general in spatially embedded networks.
The anti-glioblastoma effect of cold atmospheric plasma treatment: physical pathway v.s. chemical pathway
Authors: Yan Dayun , Wang Qihui , Malyavko Alisa , Zolotukhin Denis B. , Adhikari Manish , Sherman Jonathan H. , Keidar Michael 
Published: July 2020

AbstractCold atmospheric plasma (CAP), a near room temperature ionized gas, has shown potential application in many branches of medicine, particularly in cancer treatment. In previous studies, the biological effect of CAP on cancer cells and other mammalian cells has been based solely on the chemical factors in CAP, particularly the reactive species. Therefore, plasma medicine has been regarded as a reactive species-based medicine, and the physical factors in CAP such as the thermal effect, ultraviolet irradiation, and electromagnetic effect have been regarded as ignorable factors. In this study, we investigated the effect of a physical CAP treatment on glioblastoma cells. For the first time, we demonstrated that the physical factors in CAP could reinstate the positive selectivity on CAP-treated astrocytes. The positive selectivity was a result of necrosis, a new cell death in glioblastoma cells characterized by the leak of bulk water from the cell membrane. The physically-based CAP treatment overcomed a large limitation of the traditional chemically based CAP treatment, which had complete dependence on the sensitivity of cells to reactive species. The physically-based CAP treatment is a potential non-invasive anti-tumor tool, which may have wide application for tumors located in deeper tissues.
Respective influence of vertical mountain differentiation on debris flow occurrence in the Upper Min River, China
Authors: Ding Mingtao , Huang Tao , Zheng Hao , Yang Guohui 
Published: July 2020

AbstractThe generation, formation, and development of debris flow are closely related to the vertical climate, vegetation, soil, lithology and topography of the mountain area. Taking in the upper reaches of Min River (the Upper Min River) as the study area, combined with GIS and RS technology, the Geo-detector (GEO) method was used to quantitatively analyze the respective influence of 9 factors on debris flow occurrence. We identify from a list of 5 variables that explain 53.92%% of the total variance. Maximum daily rainfall and slope are recognized as the primary driver (39.56%) of the spatiotemporal variability of debris flow activity. Interaction detector indicates that the interaction between the vertical differentiation factors of the mountainous areas in the study area is nonlinear enhancement. Risk detector shows that the debris flow accumulation area and propagation area in the Upper Min River are mainly distributed in the arid valleys of subtropical and warm temperate zones. The study results of this paper will enrich the scientific basis of prevention and reduction of debris flow hazards.
Trends in cardiovascular risk factors in diabetic patients in comparison to general population in Iran: findings from National Surveys 2007–2016
Authors: Malekzadeh Hamid , Lotfaliany Mojtaba , Ostovar Afshin , Hadaegh Farzad , Azizi Fereidoun , Yoosefi Moein , Farzadfar Farshad , Khalili Davood 
Published: July 2020

AbstractTo determine levels of change in risk factors for cardiovascular disease among people with and without a previous diagnosis of diabetes from 2007 to 2016 in Iran. Data were obtained from five rounds of the World Health Organization STEPwise approach to Surveillance (STEPS) cross-sectional surveys. Participants were 7665 and 93,733 adults with and without known diabetes, respectively, aged 25–65 years. We used logistic and linear regressions to assess the trends of risk factors. Individuals with known diabetes compared to those without the condition, experienced greater reductions in mean levels of systolic blood pressure (3.0 vs. 0.5 mmHg among women and 3.9 vs. 1.6 mmHg among men), diastolic blood pressure (6.4 vs. 5.11 mmHg in women and 3.3 vs. 1.8 mmHg in men), and non-HDL cholesterol (42.4 vs. 27.2 mg/dL among women and 30.3 vs. 21.0 mg/dL among men) throughout these years. Men with diabetes also showed a greater reduction in the prevalence of daily cigarette smoking compared to their non-diabetic counterparts (7.3% vs. 2.3%). Fasting plasma glucose decreased among subjects with diabetes but increased among those without diabetes. Significant increases were observed in proportions who met goals for blood pressure, triglycerides, non-HDL cholesterol and LDL cholesterol in both groups; however, almost half of diabetic subjects did not achieve risk factor goals in 2016. Secondary prevention in diabetic patients was more effective than primary prevention in the general population; however, the rate of diabetic patients who met the designated goals for each risk factor was still suboptimal.
Cryopreservation of infectious Cryptosporidium parvum oocysts achieved through vitrification using high aspect ratio specimen containers
Authors: Jaskiewicz Justyna J. , Sevenler Derin , Swei Anisa A. , Widmer Giovanni , Toner Mehmet , Tzipori Saul , Sandlin Rebecca D. 
Published: July 2020

AbstractInfection with protozoa of the genus Cryptosporidium is a leading cause of child morbidity and mortality associated with diarrhea in the developing world. Research on this parasite has been impeded by many technical limitations, including the lack of cryopreservation methods. While cryopreservation of Cryptosporidium oocysts by vitrification was recently achieved, the method is restricted to small sample volumes, thereby limiting widespread implementation of this procedure. Here, a second-generation method is described for cryopreservation of C. parvum oocysts by vitrification using custom high aspect ratio specimen containers, which enable a 100-fold increase in sample volume compared to previous methods. Oocysts cryopreserved using the described protocol exhibit high viability, maintain in vitro infectivity, and are infectious to interferon-gamma (IFN-γ) knockout mice. Importantly, the course of the infection is comparable to that observed in mice infected with unfrozen oocysts. Vitrification of C. parvum oocysts in larger volumes will expedite progress of research by enabling the sharing of isolates among different laboratories and the standardization of clinical trials.
Removal of radionuclides from acidic solution by activated carbon impregnated with methyl- and carboxy-benzotriazoles
Authors: Abu-Dalo Muna A. , Nevostrueva Svetlana , Hernandez Mark 
Published: July 2020

AbstractThe removal efficiencies of metals commonly used to model the fate and transport of aqueous uranium and radioactive its daughter products, were observed on activated carbons impregnated with different benzotriazole derivatives. Acidic solutions containing U(VI), Sr(II), Eu(III), and Ce(III) were used to determine the immobilization potential of carboxybenzotriazole (CBT) and methylbenzotriazole (MeBT), where these derivatives were sorbed to different types of granular activated carbon (GAC). This sorption behavior can be predicted by Redlich–Peterson model. Flow-through column tests showed that the immobilization of uranium and some of its daughter products, significantly improves in response to oxidized GACs saturated with carboxybenzotrzole (CBT), which reached a maximum elimination for U(VI) at 260 BV, Eu(III) at 114 BV, Ce(III) at 126 BV, and Sr(II) at 100. MeBT significantly desorbed from GAC under acidic conditions. Trace amounts of CBT were observed in some column effluents, but this did not appear to alter the effectiveness of metal removal, regardless of the model radionuclide studied. These results suggest that enhanced immobilization of selected metals on GAC, can be achieved by impregnating oxidized activated carbon with carboxylated benzotriazoles, and that metal removal efficiency on this media, is related to their valence and ionic radius in acidic environments.
Negative feedback regulation by HuR controls TRIM21 expression and function in response to UV radiation
Authors: Guha Abhishek , Nag Sharanya , Ray Partho Sarothi 
Published: July 2020

AbstractThe E3 ubiquitin ligase TRIM21 plays a crucial role as a negative regulator of innate immune responses. Recent evidence has also indicated the involvement of TRIM21 in the genotoxic stress response and suppressing tumorigenesis. Our previous work has demonstrated a new function of TRIM21 in inhibiting p53 protein synthesis by degrading the RNA-binding protein HuR in response to UV radiation. This suggested a pro-oncogenic role of TRIM21. In this study, we have shown that TRIM21 enhances the proliferation of MCF7 breast carcinoma cells and counteracts the decrease in cell proliferation and colony formation caused by UV-induced DNA damage. Further, this pro-oncogenic role of TRIM21 in response to DNA damage is mediated by its degradation of HuR. Conversely, we found that HuR binds to a U-rich element in the 3′UTR of TRIM21 mRNA and activates its translation, thereby constituting a negative feedback loop. We found that dihydrotanshinone-I (DHTS-I), a plant-derived product which prevents HuR binding to specific RNAs, prevented HuR-mediated upregulation of TRIM21, while increasing the HuR-mediated upregulation of p53. Together, these findings demonstrate a negative feedback regulation between TRIM21 and HuR, which may play an important role in regulating the level of p53 in the genotoxic stress response.
Geobiochemistry characteristics of rare earth elements in soil and ground water: a case study in Baotou, China
Authors: Tang Shuting , Zheng Chunli , Chen Minjie , Du Weiqi , Xu Xin 
Published: July 2020

AbstractThe distribution of rare earth elements and the microbial community in nearby ground water and soil were influenced by tailings ponds. Accordingly, the behaviors of rare earth elements in ground water and soil around the tailings pond, and the changes of microbial communities were both investigated in this study. The results showed that rare earth elements accumulated in ground water and soil around the tailings pond appeared as light rare earth elements enrichment. Through the normalization of rare earth elements, different extents of anomaly (from negative to positive) were observed for Ce and Eu in the distribution patterns of REEs in groundwater, however, Ce and Eu were negatively anomaly in soil. According to the correlation analysis, Mn2+, SO42−, Cl−, ammonia nitrogen and Ca2+ are significantly correlated with the distribution of rare earth elements. Meanwhile, there were the same dominant bacteria in ground water and soil including Actinobateria, Proteobacteria and Acidobacteria at the phylum level. This microbial community composition is similar to that reported in arid lands around the world. On the other hand, Bacillus and Blastococcus showed significant correlation with rare earth elements at the genus level. This study might provide an important basis for the risk assessment of REEs in the environment.