bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic characterization of anemone‐type chrysanthemum (Chrysanthemum morifolium) using floral morphology and SRAP markers
Authors: Yang Xiaodong , Ao Ni , Qu Yixin , Wu Yangyang , Su Jiangshuo , Ding Lian , Chen Sumei , Jiang Jiafu , Guan Zhiyong , Chen Fadi , Fang Weimin , Zhang Fei 
Published: November 2019

AbstractAn appreciation of the genetic diversity retained in the gene pool of anemone‐type chrysanthemums is needed to drive further genetic improvement of this commercially important ornamental species. Here, a panel of 67 accessions was characterized with respect to both floral morphology, assessed using a set of 12 inflorescence‐associated traits, and to genotype, using sequence‐related amplified polymorphism (SRAP) fingerprinting. Abundant variation and a high level of broad‐sense heritability (>88%) were revealed for all traits except number of tubular floret lobes. Of the possible 66 pairwise inter‐trait combinations, 37 proved to be significantly correlated. A principal component analysis of the trait data showed that the first four principal components explained ~78% of the variance; meanwhile a hierarchical clustering analysis revealed a number of discrete groups reflecting the various cultivated types, but was not predictive of provenance. The SRAP genotyping employed 22 primer combinations and generated 238 informative fragments. A moderately high level of the Jaccard's similarity coefficient (mean 0.67) was calculated for the examined panel. A hierarchical clustering analysis based on the genotypic data revealed four groups, which were clearly reflective of cultivated type; moreover, a comparison of the within‐cluster means for each of the two key anemone‐related traits—tubular floret length and tubular floret length/style length—showed that the genotype‐based clusterings were associated with the anemone levels, albeit imperfectively. A principal coordinate analysis showed that the first two coordinates accounted for about 14% of the total variation. In summary, the present study adds new understanding of the genetic diversity present in the anemone‐type chrysanthemum gene pool, and the findings will lay the foundation for implementing a genotype/phenotype association strategy to reveal the genetic and molecular basis of floral form in this valuable ornamental species.
Fluence Rate Determines PDT Efficiency in Breast Cancer Cells Displaying Different GSH Levels
Authors: dos Santos Ancély F. , de Almeida Daria Raquel Q. , Terra Letícia F. , Wailemann Rosangela A.M. , Gomes Vinícius M. , Arini Gabriel S. , Ravagnani Felipe G. , Baptista Maurício S. , Labriola Leticia 
Published: November 2019

AbstractPhotodynamic therapy (PDT) appears as a promising alternative in the treatment of breast cancer since it can be highly effective in curing cancer while preserving normal tissue. However, predicting outcomes in PDT still constitutes a great challenge. One of the parameters that are usually empirically determined is the rate of photon flux delivered to the tissue (light fluence rate). In the present study, we intended to understand why monolayers of human cells derived from mammary adenocarcinomas (MDA‐MB‐231 and MCF‐7) respond quite differently to fluence rates (cells were irradiated either for 6 or for 16 min) at a fixed light dose (4.5 J cm−2) delivered with an array of LEDs in a typical methylene blue PDT protocol. While death rates of MDA‐MB‐231 cells were insensitive to the fluence rate, MCF‐7 cells showed a quite impressive (three times) decrease in cell death levels in the shorter irradiation protocol. Independent on cell type cell death was invariably correlated with the depletion of reduced glutathione intracellular levels and consequently with widespread redox misbalance. Our data show the potential to optimize fluence rates to provide exhaustion of the cell antioxidant responses in order to circumvent therapy resistance of breast tumors.
Containment and Connectivity in Dutch Urban Systems: A Network‐Analytical Operationalisation of the Three‐Systems Model
Authors: Poorthuis Ate , van Meeteren Michiel 
Published: November 2019

AbstractThis paper discusses key methodological issues with nodalising interaction data of urban networks to produce a state‐of‐the‐art settlement geography of the Netherlands. We operationalise the three‐systems model that analyses functional settlement geographies through the interaction between the daily urban system, the central place system and the export base system. We utilise theoretically‐informed selections of spatial interactions derived from travel survey data at the finely‐grained postcode level. After examining the methodological challenge of the node‐inclusivity dilemma, we estimate the causal mechanisms that geographically structure each system and determine which spatial interactions should be assigned to nodes (containment) and edges (connectivity). The three systems produce different regionalisations that are neither mutually exclusive nor perfectly nested. Further analysis of the multiplexity of the three systems reveals the importance of the imbricated boundaries between the urban subsystems. We argue that these interplaces deserve more attention as they are particularly sensitive to changes in urbanisation trends.
The role of frozen–thawed embryo replacement cycles in assisted conception
Authors: Noble Matt , Child Tim 
Published: November 2019

Key content

Recent years have seen a dramatic rise in the number of frozen–thawed embryo replacement (FER) cycles.

Improved embryo cryopreservation techniques have resulted in outcomes for FER that are similar to fresh embryo transfer.

FER maximises the cumulative live‐birth rate of a fresh in vitro fertilisation (IVF) cycle by using excess embryos and encourages a policy of single embryo transfer; this has contributed to a fall in the multiple pregnancy rate associated with IVF.

Freezing all suitable embryos in a fresh cycle reduces the risk of ovarian hyperstimulation syndrome.

The endometrium is prepared for FER by a natural or medicated protocol; the optimum method is unknown.

Learning objectives

To appreciate the importance of FER in IVF practice.

To understand the current debate surrounding fresh versus frozen IVF.

To understand the indications for, and methods of, embryo cryopreservation.

To understand the commonly used methods of endometrial preparation for FER.

To be able to date an FER pregnancy.

Ethical issues

Funding from the National Health Service (NHS) for embryo storage and FER varies widely across the UK; where funding is limited, this can result in viable embryos being discarded.

When an embryo is created by a couple, the consent of both partners is required before transferring the embryo.
Genomic and transcriptomic profiling of carcinogenesis in patients with familial adenomatous polyposis
Authors: Li Jingyun , Wang Rui , Zhou Xin , Wang Wendong , Gao Shuai , Mao Yunuo , Wu Xinglong , Guo Limei , Liu Haijing , Wen Lu , Fu Wei , Tang Fuchou 
Published: November 2019

ObjectiveFamilial adenomatous polyposis (FAP) is characterised by the development of hundreds to thousands of adenomas at different evolutionary stages in the colon and rectum that will inevitably progress to adenocarcinomas if left untreated. Here, we investigated the genetic alterations and transcriptomic transitions from precancerous adenoma to carcinoma.DesignWhole-exome sequencing, whole-genome sequencing and single-cell RNA sequencing were performed on matched adjacent normal tissues, multiregionally sampled adenomas at different stages and carcinomas from six patients with FAP and one patient withMUTYH-associated polyposis (n=56 exomes, n=56 genomes and n=8,757 single cells). Genomic alterations (including copy number alterations and somatic mutations), clonal architectures and transcriptome dynamics during adenocarcinoma carcinogenesis were comprehensively investigated.ResultsGenomic evolutionary analysis showed that adjacent lesions from the same patient with FAP can originate from the same cancer-primed cell. In addition, the tricarboxylic acid cycle pathway was strongly repressed in adenomas and was then slightly alleviated in carcinomas. Cells from the ‘normal’ colon epithelium of patients with FAP already showed metabolic reprogramming compared with cells from the normal colon epithelium of patients with sporadic colorectal cancer.ConclusionsThe process described in the previously reported field cancerisation model also occurs in patients with FAP and can contribute to the formation of adjacent lesions in patients with FAP. Reprogramming of carbohydrate metabolism has already occurred at the precancerous adenoma stage. Our study provides an accurate picture of the genomic and transcriptomic landscapes during the initiation and progression of carcinogenesis, especially during the transition from adenoma to carcinoma.
Aquifer-eustasy as the main driver of short-term sea-level fluctuations during Cretaceous hothouse climate phases
Authors: Sames Benjamin , Wagreich M. , Conrad C. P. , Iqbal S. 
Published: November 2019

Abstract

A review of short-term (<3 myr:
c.
100 kyr to 2.4 myr) Cretaceous sea-level fluctuations of several tens of metres indicates recent fundamental progress in understanding the underlying mechanisms for eustasy, both in timing and in correlation. Cretaceous third- and fourth-order hothouse sea-level changes, the sequence-stratigraphic framework, are linked to Milankovitch-type climate cycles, especially the longer-period sequence-building bands of 405 kyr and 1.2 myr. In the absence of continental ice sheets during Cretaceous hothouse phases (e.g. Cenomanian–Turonian), growing evidence indicates groundwater-related sea-level cycles: (1) the existence of Milankovitch-type humid-arid climate oscillations, proven via intense humid weathering records during times of regression and sea-level lowstands; (2) missing or inverse relationships of sea-level and the marine δ
18
O archives, i.e. the lack of a pronounced positive excursion, cooling signal during sea-level lowstands; and (3) the anti-phase relationship of sea and lake levels, attesting to high groundwater levels and charged continental aquifers during sea-level lowstands. This substantiates the aquifer-eustasy hypothesis. Rates of aquifer-eustatic sea-level change remain hard to decipher; however, reconstructions range from a very conservative minimum estimate of 0.04 mm a
−1
(longer time intervals) to 0.7 mm a
−1
(shorter, probably asymmetric cycles). Remarkably, aquifer-eustasy is recognized as a significant component for the Anthropocene sea-level budget.
An introduction to causes and consequences of Cretaceous sea-level changes (IGCP 609)
Authors: Wagreich Michael , Sames Benjamin , Hart Malcolm , Yilmaz Ismail O. 
Published: November 2019

Abstract
The International Geoscience Programme Project IGCP 609 addressed correlation, causes and consequences of short-term sea-level fluctuations during the Cretaceous. Processes causing several ka to several Ma (third- to fourth-order) sea-level oscillations during the Cretaceous are so far poorly understood. IGCP 609 proved the existence of sea-level cycles during potential ice sheet-free greenhouse to hothouse climate phases. These sea-level fluctuations were most probably controlled by aquifer-eustasy that is altering land-water storage owing to groundwater aquifer charge and discharge. The project investigated Cretaceous sea-level cycles in detail in order to differentiate and quantify both short- and long-term records based on orbital cyclicity. High-resolution sea-level records were correlated to the geological timescale resulting in a hierarchy of sea-level cycles in the longer Milankovitch band, especially in the 100 ka, 405 ka, 1.2 Ma and 2.4 Ma range. The relation of sea-level highs and lows to palaeoclimate events, palaeoenvironments and biota was also investigated using multiproxy studies. For a hothouse Earth such as the mid-Cretaceous, humid–arid climate cycles controlling groundwater-related sea-level change were evidenced by stable isotope data, correlation to continental lake-level records and humid–arid weathering cycles.
Beclin 1 Promotes Endosome Recruitment of Hepatocyte Growth Factor Tyrosine Kinase Substrate to Suppress Tumor Proliferation
Authors: Matthew-Onabanjo Asia N. , Janusis Jenny , Mercado-Matos Jose , Carlisle Anne E. , Kim Dohoon , Levine Fayola , Cruz-Gordillo Peter , Richards Ryan , Lee Michael J. , Shaw Leslie M. 
Published: November 2019

AbstractBeclin 1 has nonautophagic functions that include its ability to regulate endocytic receptor trafficking. However, the contribution of this function to tumor suppression is poorly understood. Here, we provide in vivo evidence that Beclin 1 suppresses tumor proliferation by regulating the endocytic trafficking and degradation of the EGFR and transferrin (TFR1) receptors. Beclin 1 promoted endosomal recruitment of hepatocyte growth factor tyrosine kinase substrate (HRS), which was necessary for sorting surface receptors to intraluminal vesicles for signal silencing and lysosomal degradation. In tumors with low Beclin 1 expression, endosomal HRS recruitment was diminished and receptor function was sustained. Collectively, our results demonstrate a novel role for Beclin 1 in impeding tumor growth by coordinating the regulation of key growth factor and nutrient receptors. These data provide an explanation for how low levels of Beclin 1 facilitate tumor proliferation and contribute to poor cancer outcomes.Significance:Beclin 1 controls the trafficking fate of growth regulatory receptors to suppress tumor proliferation.
New Case Detection by Cascade Testing in Familial Hypercholesterolemia
Authors: Lee Christopher , Rivera-Valerio Miriannie , Bangash Hana , Prokop Larry , Kullo Iftikhar J. 
Published: November 2019

Background:
The prevalence of familial hypercholesterolemia is 1 in 250, but <10% of patients are diagnosed. Cascade testing enables early detection of cases through systematic family tracing. Establishment of familial hypercholesterolemia cascade testing programs in the US could be informed by approaches used elsewhere.

Methods:
We conducted a systematic review of published studies in the English language of cascade testing for familial hypercholesterolemia, which reported the number of index cases and number of relatives tested and specified methods of contacting relatives and testing modalities methods utilized. For each study, we calculated yield (proportion of relatives who test positive) and new cases per index case, to facilitate comparison.

Results:
We identified 10 studies from the literature that met inclusion criteria; the mean number of probands and relatives per study was 242 and 826, respectively. The average yield was 44.76% with a range of 30% to 60.5%, and the mean new cases per index case was 1.65 with a range of 0.22 to 8.0. New cases per index case tended to be greater in studies that used direct contact versus indirect contact (2.06 versus 0.86), tested beyond first-degree relatives versus only first-degree relatives (3.65 versus 0.80), used active sample collection versus collection at clinic (4.11 versus 1.06), and utilized genetic testing versus biochemical testing (2.47 versus 0.42).

Conclusions:
New case detection in familial hypercholesterolemia cascade testing programs tended to be higher with direct contact of relatives, testing beyond first-degree relatives, in-home–based sample collection, and genetic testing. These findings should be helpful for establishing cascade testing programs in the United States.
Improving Radar Echo Lagrangian Extrapolation Nowcasting by Blending Numerical Model Wind Information: Statistical Performance of 16 Typhoon Cases
Authors: Chung Kao-Shen , Yao I-An 
Published: November 2019

Abstract
Severe weather nowcasting is a crucial mission of atmospheric science for the betterment of society to save life, limb, and property. In this study, composite radar data from the Central Weather Bureau of 16 typhoons are collected to examine the statistical performance of the McGill Algorithm for Precipitation nowcasting using Lagrangian Extrapolation (MAPLE) over Taiwan, an extrapolation algorithm that predicts future precipitation based on current radar echoes. In addition, instead of mixing the precipitation between radar extrapolation and numerical model forecast as in previous studies, a blending system is formed by synthesizing the wind information from model forecast with the echo extrapolation motion field via a variational algorithm to improve the nowcasting system. The statistical results of the radar echo extrapolation for 16 typhoon cases show that while the quantitative precipitation nowcasting skill can persist for up to 2 h, significant distortion for the rotational system is found after 2 h. On the other hand, the blending system helps to capture and maintain the rotation of typhoon rainband structures. The blending system extends the nowcasting skill by 1 h to a total of 3 h. Furthermore, the blending scheme performs especially well after the typhoon makes landfall in Taiwan. For disaster prevention and mitigation, this blending nowcasting technique may provide effective weather information immediately.
Staying Safe in a Tornado: A Qualitative Inquiry into Public Knowledge, Access, and Response to Tornado Warnings
Authors: Walters Jayme E. , Mason Lisa Reyes , Ellis Kelsey , Winchester Betsy 
Published: November 2019

Abstract
Tornadoes in the southeastern United States continue to cause substantial injury, death, and destruction. The present study seeks to 1) understand inadequate warning access, less understanding, and/or less likelihood of responding to tornado warnings; 2) examine public attitudes about NWS communications; and 3) explore the perceptions of NWS personnel regarding public response to tornado warnings, factors that might influence response, and how their perceptions impact their communication. Participants include a purposive sample of NWS forecasters in Tennessee (n = 11) and residents (n = 45) who were identified as having low access to, low knowledge of, or an unsafe response to tornado warnings in a previous study. A qualitative approach with semistructured interviews was used. Findings indicated that most participants had at least one warning source. Barriers to warning access included electricity outages, rurality, lack of storm radio, heavy sleeping, and hearing impairments. Most participants had knowledge of NWS guidelines for safe shelter seeking but still engaged in behaviors considered unsafe. Proximity, personal experience, and influence of family and friends emerged as influencers of response to warnings. NWS personnel perceived that proximity played a significant role in shelter-seeking behavior as well as the need for confirmation. Poor access to safe shelter arose as a major concern for NWS personnel, specifically mobile home residents. Messaging and specificity in warnings to evoke safe shelter-seeking behavior surfaced as critical issues for NWS personnel. Implications for education and policy changes to enhance public safety and improve public health are noted.
Developmentally appropriate patient education during transition: A study of healthcare providers’ and parents’ perspective
Authors: Morsa Maxime , Gagnayre Rémi , Pomey Marie-Pascale , Deccache Carole , Lombrail Pierre 
Published: November 2019

Introduction: Patient education is recommended to improve the transition from paediatric to adult care for young people with chronic conditions. But a consensus has not been reached regarding a particular model. This study aimed to understand how to prepare for the implementation of a Developmentally Appropriate Patient Education during Transition (DAPET), which would revolve around the young person’s psychosocial development. Method: Three focus groups were organised with healthcare providers and two focus groups took place with the parents of young people with chronic conditions. We used activity theory to explore practices and to identify obstacles to the implementation of DAPET, as well as to recognise which resources might be available to implement DAPET. Results: Healthcare providers agreed on the need to engage in an educational approach centred on the psychosocial development of young people during transition. However, study findings highlight the following obstacles to doing so: a lack of competencies in adolescent and young adult medicine and a lack of available resources to meet these goals. Furthermore, parents wanted to redefine their role in the transition process and to allow their children to develop self-management skills. Conclusion: Healthcare providers and parents considered the implementation of DAPET to be acceptable and even advisable. However, the programme’s feasibility was questioned due to perceived shortfalls in the hospital system as it currently stands and the ways in which an educational approach would be applied. An environment that facilitates healthcare providers’ educational initiatives and encourages the participation of parents is required.
Consumer marketplace lending in Australia: Credit scores and loan funding success
Authors: Grant Andrew , Deer Luke 
Published: November 2019

This article examines borrower acceptance in consumer marketplace lending using a unique dataset from the largest platform in Australia, Society One. Applications are initially filtered through an automated decision tree based on a third-party Veda (Equifax) credit score. At the second stage of assessment, loan applications are underwritten by the platform before being offered to sophisticated investors for purchase. The platform accepts around 11% of completed applications, with around 55% declined by an automated decision process and the remaining 34% by the manual underwriting process. More than 80% of purchased loans were made to borrowers with credit scores classed as ‘Good’, ‘Very Good’ or ‘Excellent’ (the threshold for ‘Good’ being a score of 622). However, underwriters decline around two-thirds of these higher credit score applicants, showing the importance of the underwriting process to the platform’s growth. JEL Classification: G21, G23, D14, D45, D82