bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of a cellulose synthase catalytic subunit from the oomycete pathogen of crops Phytophthora capsici
Authors: Pang Zhili , McKee Lauren S. , Srivastava Vaibhav , Klinter Stefan , Díaz-Moreno Sara M. , Orlean Peter , Liu Xili , Bulone Vincent 
Published: August 2020

AbstractPhytophthora capsici Leonian is an important oomycete pathogen of crop vegetables, causing significant economic losses each year. Its cell wall, rich in cellulose, is vital for cellular integrity and for interactions with the host organisms. Predicted cellulose synthase (CesA) proteins are expected to catalyze the polymerization of cellulose, but this has not been biochemically demonstrated in an oomycete. Here, we present the properties of the four newly identified CesA proteins from P. capsici and compare their domain organization with that of CesAs from other lineages. Using a newly constructed glucosyltransferase-deficient variant of Saccharomyces cerevisiae with low residual background activity, we have achieved successful heterologous expression and biochemical characterization of a CesA protein from P. capsici (PcCesA1). Our results demonstrate that the individual PcCesA1 enzyme produces cellobiose as the major reaction product. Co-immunoprecipitation studies and activity assays revealed that several PcCesA proteins interact together to form a complex whose multiproteic nature is most likely required for cellulose microfibril formation. In addition to providing important insights into cellulose synthesis in the oomycetes, our data may assist the longer term identification of cell wall biosynthesis inhibitors to control infection by pathogenic oomycetes.
Consumer Law and Policy Relating to Change of Circumstances Due to the COVID-19 Pandemic
Authors:   COVID-19- Consumer Law Research Group, Alderman Richard , De Franceschi Alberto , Giancaspro Mark , Howells Geraint , Lei Chen , Lete Javier , Micklitz Hans-W , Miscenic Emilia , Naude Tjakie , Pichonnaz Pascal , Poillot Elise , Ramsay Iain , Terryn Evelyne , Twigg-Flesner Christian , Wilhelmsson Thomas 
Published: August 2020

AbstractAn unprecedented number of consumer problems has been caused by the COVID-19 pandemic, not least with regard to refunds of prepayments and the ability of consumers to keep up their monthly payments under loan and rental agreements. Based on a notion of societal force majeure sketched in this paper, we propose guiding principles in respect of the introduction of moratoria on recurring payments, the use of refunds or vouchers in respect of prepayments, and associated enforcement challenges. This analysis draws on experiences around the globe.
Seismic Risks: a Criminological Analysis of European Investment Bank Support for the Castor Project
Authors: Beizsley Daniel 
Published: August 2020

AbstractThe Castor project was a proposed underground gas storage facility off Spain’s Mediterranean coast financed through a public–private partnership (PPP) between the Spanish state and a consortium led by the Spanish conglomerate Grupo ACS. During the project’s development, cost overruns led to it being refinanced through a bond issuance organized by the European Investment Bank (EIB)—the finance arm of the European Union (EU). On reaching the injection stage, gas escaped violently causing hundreds of small-scale earthquakes resulting in the project’s closure and, as per the terms of the concession, the generation of a €1.3 billion debt for Spanish gas consumers. Using criminological concepts and theory, this article will first explore how the seismic risks that the project posed were excluded from the EIB appraisal process before identifying the causes of the disaster as a product of the “financialization” of infrastructure and investor protection frameworks needed to sustain it.
Experimental Study of Self-heating Ignition of Lithium-Ion Batteries During Storage: Effect of the Number of Cells
Authors: He Xuanze , Restuccia Francesco , Zhang Yue , Hu Zhenwen , Huang Xinyan , Fang Jun , Rein Guillermo 
Published: August 2020

AbstractLithium-ion batteries (LIBs) are widely used as energy storage devices. However, a disadvantage of these batteries is their tendency to ignite and burn, thereby creating a fire hazard. Ignition of LIBs can be triggered by abuse conditions (mechanical, electrical or thermal abuse) or internal short circuit. In addition, ignition could also be triggered by self-heating when LIBs are stacked during storage or transport. However, the open circuit self-heating ignition has received little attention and seems to be misunderstood in the literature. This paper quantifies the self-heating behaviour of LIB by means of isothermal oven experiments. Stacks of 1, 2, 3 and 4 Sanyo prismatic LiCoO2 cells at 30% state of charge were studied. The surface and central temperatures, voltage, and time to ignition were measured. Results show that self-heating ignition of open circuit LIBs is possible and its behaviour has three stages: heating up, self-heating and thermal runaway. We find for the first time that, for this battery type, as the number of cells increases from 1 to 4, the critical ambient temperature decreases from 165.5°C to 153°C. A Frank-Kamenetskii analysis using the measured data confirms that ignition is caused by self-heating. Parameters extracted from Frank-Kamenetskii theory are then used to upscale the laboratory results, which shows large enough LIB ensembles could self-ignite at even ambient temperatures. This is the first experimental study of the effect of the number of cells on self-heating ignition of LIBs, contributing to the understanding of this new fire hazard.
Palliative care needs-assessment and measurement tools used in patients with heart failure: a systematic mixed-studies review with narrative synthesis
Authors: Remawi Bader Nael , Gadoud Amy , Murphy Iain Malcolm James , Preston Nancy 
Published: August 2020

AbstractPatients with heart failure have comparable illness burden and palliative care needs to those with cancer. However, few of them are offered timely palliative care. One main barrier is the difficulty in identifying those who require palliative care. Several palliative care needs-assessment/measurement tools were used to help identify these patients and assess/measure their needs, but it is not known which one is the most appropriate for this population. This review aimed to identify the most appropriate palliative care needs-assessment/measurement tools for patients with heart failure. Cochrane Library, MEDLINE Complete, AMED, PsycINFO, CINAHL Complete, EMBASE, EThOS, websites of the identified tools, and references and citations of the included studies were searched from inception to 25 June 2020. Studies were included if they evaluated palliative care needs-assessment/measurement tools for heart failure populations in terms of development, psychometrics, or palliative care patient/needs identification. Twenty-seven papers were included regarding nineteen studies, most of which were quantitative and observational. Six tools were identified and compared according to their content and context of use, development, psychometrics, and clinical applications in identifying patients with palliative care needs. Despite limited evidence, the Needs Assessment Tool: Progressive Disease – Heart Failure (NAT:PD-HF) is the most appropriate palliative care needs-assessment tool for use in heart failure populations. It covers most of the patient needs and has the best psychometric properties and evidence of identification ability and appropriateness. Psychometric testing of the tools in patients with heart failure and evaluating the tools to identify those with palliative care needs require more investigation.
Weight-Based Discrimination in the Italian Labor Market: an Analysis of the Interaction with Gender and Ethnicity
Authors: Busetta Giovanni , Campolo Maria Gabriella , Panarello Demetrio 
Published: August 2020

AbstractAccess to the Italian job market is undermined by several kinds of discrimination influencing the opportunities for individuals to obtain a job. In this study, we analyze together the impact of three of the most relevant kinds of discrimination operating in the Italian labor market: gender, race, and weight. Our aim is to assess whether gender and race either increase or decrease the impact of weight-based discrimination. In this respect, we submit a set of fictitious résumés including photos of either obese or thin applicants in response to real online job offers. Our results indicate that the strongest kind of discrimination operating in the Italian labor market is the one connected to the candidate’s geographical origin. Moreover, we find discrimination based on body weight to be more relevant within immigrants than within natives, and gender gap appears to be higher within the obese candidates’ group compared to the normal-weight candidates’ one. This last result is particularly relevant, as the growing rates of obesity forecasted for the next years could in turn produce an increase in the gender gap, which in Italy is already massive.
Anti-Leukemia Activity of Au/CuO/ZnO Nanoparticles Synthesized used Verbena officinalis Extract
Authors: Dobrucka Renata , Romaniuk-Drapała Aleksandra , Kaczmarek Mariusz 
Published: August 2020

AbstractAs biological synthesis has become an alternative to chemical and physical methods for synthesizing nanoparticles, this work describes the synthesis of Au/CuO/ZnO nanoparticles using Verbena officinalis extract. The synthesized Au/CuO/ZnO nanoparticles were characterized using Ultraviolet–Visible, Fourier Transform-Infrared, Transmission Electron Microscopy and Atomic Force Microscopy. The influence of Au/CuO/ZnO nanoparticles on cell viability was evaluated in vitro, using the established cell line – Jurkat (ATCC® TIB-152™). The Annexin V binding test confirmed the previous results of the MTT assay, which indicate that the studied complex of Au/CuO/ZnO nanoparticles has a strong cytotoxic effect on the Jurkat cell line. The type of death and the effectiveness of cell elimination depended both on the concentration of the complex and the duration of culture.
Twisted characters and holomorphic symmetries
Authors: Saberi Ingmar , Williams Brian R. 
Published: August 2020

AbstractWe consider holomorphic twists of arbitrary supersymmetric theories in four dimensions. Working in the BV formalism, we rederive classical results characterizing the holomorphic twist of chiral and vector supermultiplets, computing the twist explicitly as a family over the space of nilpotent supercharges in minimal supersymmetry. The BV formalism allows one to work with or without auxiliary fields, according to preference; for chiral superfields, we show that the result of the twist is an identical BV theory, the holomorphic $$\beta \gamma $$

β
γ

system with superpotential, independent of whether or not auxiliary fields are included. We compute the character of local operators in this holomorphic theory, demonstrating agreement of the free local operators with the usual index of free fields. The local operators with superpotential are computed via a spectral sequence and are shown to agree with functions on a formal mapping space into the derived critical locus of the superpotential. We consider the holomorphic theory on various geometries, including Hopf manifolds and products of arbitrary pairs of Riemann surfaces, and offer some general remarks on dimensional reductions of holomorphic theories along the $$(n-1)$$

(
n
-
1
)

-sphere to topological quantum mechanics. We also study an infinite-dimensional enhancement of the flavor symmetry in this example, to a recently studied central extension of the derived holomorphic functions with values in the original Lie algebra, that generalizes the familiar Kac–Moody enhancement in two-dimensional chiral theories.
Engineering the pH-Sensitivity of the Graphene and Carbon Nanotube Based Nanomedicines in Smart Cancer Therapy by Grafting Trimetyl Chitosan
Authors: Khoshoei Azadeh , Ghasemy Ebrahim , Poustchi Fatemeh , Shahbazi Mohammad-Ali , Maleki Reza 
Published: August 2020

Abstract
Purpose
The aim of this study was to introduce a smart and responsive drug carrier for Doxorubicin (DOX) and Paclitaxel (PAX) for desirable therapeutic application.

Method
Loading and releasing of DOX and PAX from smart and pH-sensitive functionalized single-walled carbon nanotube (SWCNTs) and graphene carriers have been simulated by molecular dynamics. The influences of chitosan polymer on proposed carriers have been studied, and both carriers were functionalized with carboxyl groups to improve the loading and releasing properties of the drugs.

Results
The results showed that DOX could be well adsorbed on both functionalized SWCNTs and graphene. In contrast, there was a weak electrostatic and Van der Waals interaction between both these drugs and carriers at cancerous tissues, which is highly favorable for cancer therapy. Adding trimethyl chitosan (TMC) polymer to carriers facilitated DOX release at acidic tissues. Furthermore, at blood pH, the PAX loaded on the functionalized SWCNTs carrier represented the highest dispersion of the drug while the DOX-graphene showed the highest concentration of the drug at a point. In addition, the mean-square displacement (MSD) results of PAX-graphene indicated that the PAX could be adsorbed quickly and be released slowly. Finally, functionalized graphene-TMC-PAX is a smart drug system with responsive behavior and controllable drug release, which are essential in cancer therapy.

Conclusion
Simultaneous application of the carboxyl group and TMC can optimize the pH sensitivity of the SWCNTs and graphene to prepare a novel and smart drug carrier for cancer therapy.
Pros and cons of using a standard protocol to test germination of alpine species
Authors: Margreiter Vera , Pagitz Konrad , Berg Christian , Schwager Patrick , Erschbamer Brigitta 
Published: August 2020

AbstractStoring seeds in seed banks is an effective way to preserve plant diversity and conserve species. An essential step towards a valuable conservation is the validation of germination. This study presents a germination screening of seeds from 255 species of the European Eastern Alps, which were to be stored at the Millennium Seed Bank (Kew, UK). The final germination percentage (FGP) was determined using a standard protocol in the laboratory. Species were classified according to species rarity, plant community, occurrence at elevation belts, bedrock types, as well as CSR strategies, and further, seed mass was examined. We could not find statistically significant differences of FGP within these classes, but 74.9% of all tested species germinated using the standard protocol, and half of them had FGP ≥ 20.1–100%. A treatment with gibberellic acid enhanced the germination in half of the species to which this treatment was applied. Common families in alpine regions, i.e. Asteraceae, Poaceae and Saxifragaceae were highlighted in terms of their germination behaviour. The results provide an evaluation of the application of standard protocols to a broad Alpine species pool on the one hand, and on the other hand, provide ecological insights of the species tested. Germination is not only one of the most important events of the reproductive cycle of plants but could also be a key feature in species' responses to changing environmental conditions.
Internal Herniation Incidence After RYGB and the Predictive Ability of a CT Scan as a Diagnostic Tool
Authors: Torensma Bart , Kooiman Laurens , Liem Ronald , Monpellier Valerie M. , Swank Dingeman J. , Tseng Larissa 
Published: August 2020

Abstract
Purpose
The clinical diagnosis of an internal herniation (IH) after a Roux-en-Y Gastric Bypass (RYGB) remains difficult; therefore, performing a CT scan is usually part of the diagnostic process. The goal of this study was to assess the incidence of IH in patients with open and closed MD (mesenteric defect) and to study if the ability to diagnose an IH with a CT scan is different between these groups.

Materials and Methods
IH was defined as a visible intestine through the mesenteric defect underneath the jejunojejunostomy and/or in the Petersen’s space. CT scan outcomes were compared with the clinical diagnosis of an IH. Until 31 June 2013, standard care was to leave mesenteric defects (MDs) open; after this date, they were always closed.

Results
The incidence of IH in the primarily non-closed group was 3.9%, and in the primarily closed group, this was 1.3% (p = 0.001). In group A (non-closed MD and CT), the sensitivity of the CT scan was 80%, and specificity was 0%. In group C (closed MD and CT), the sensitivity was 64.7%, and specificity was 89.5%. In group B (non-closed, no CT), an IH was visible in 58.7% of the cases and not in 41.3%. In group D (only a re-laparoscopy), an IH was visible in 34.3% of the cases and not in 65.7%.

Conclusions
Using the CT scan in suspected IH is not useful in if the MDs were not closed. If the MDs were closed, then a CT scan is predictive for the diagnosis IH.
Impacts of Water Clarity Variability on Temperature and Biogeochemistry in the Chesapeake Bay
Authors: Kim Grace E. , St-Laurent Pierre , Friedrichs Marjorie A. M. , Mannino Antonio 
Published: August 2020

AbstractEstuarine water clarity depends on the concentrations of aquatic constituents, such as colored dissolved organic matter, phytoplankton, inorganic suspended solids, and detritus, which are influenced by variations in riverine inputs. These constituents directly affect temperature because when water is opaque, sunlight heats a shallower layer of the water compared to when it is clear. Despite the importance of accurately predicting temperature variability, many numerical modeling studies do not adequately account for this key process. In this study, we quantify the effect of water clarity on heating by comparing two simulations of a hydrodynamic-biogeochemical model of the Chesapeake Bay for the years 2001–2005, in which (1) water clarity is constant in space and time for the computation of solar heating, compared to (2) a simulation where water clarity varies with modeled concentrations of light-attenuating materials. In the variable water clarity simulation, the water is more opaque, particularly in the northern region of the Bay. This decrease in water clarity reduces the total heat, phytoplankton, and nitrate throughout the Bay. During the spring and summer months, surface temperatures in the northern Bay are warmer by 0.1 °C and bottom temperatures are colder by 0.2 °C in the variable light attenuation simulation. Warmer surface temperatures encourage phytoplankton growth and nutrient uptake near the head of the Bay, and fewer nutrients are transported downstream. These impacts are greater during higher river flow years, when differences in temperature, nutrients, phytoplankton, and zooplankton extend further seaward compared to other years. This study demonstrates the consequences of utilizing different light calculations for estuarine heating and biogeochemistry.
Retained Functionality of Atherosclerotic Human Arteries Following Photoactivated Linking of the Extracellular Matrix by Natural Vascular Scaffolding Treatment
Authors: Ansari Ejaz , Anderson Blake , Kauser Katalin 
Published: August 2020

AbstractIn this study, we investigated natural vascular scaffolding (NVS) treatment on vascular functionality using freshly isolated human popliteal arteries in vitro. Arteries were exposed to intraluminal NVS treatment consisting of a compound (4 amino-1,8-naphthalimide) photoactivated by a 450-nm light-emitting light fiber placed inside the artery. This procedure results in covalent linking between the extracellular matrix proteins to achieve a larger vessel diameter post-angioplasty and minimizing elastic recoil. Immediately following NVS treatment, rings were cut from the treated arteries and mounted in organ baths for contractility testing in response to U46619 and sodium nitroprusside. We also investigated the effect of NVS treatment on IL-6 cytokine release from vascular rings following a 4-h organoculture post-NVS treatment. Based on our results, we conclude that exposure of the vessels to NVS treatment does not adversely affect the contractile responsiveness of the vascular smooth muscle and exerts no pro-inflammatory effect.
The role of adjuvant radiotherapy in patients with malignant phyllodes tumor of the breast: a propensity-score matching analysis
Authors: Zhao Wen , Tian Qi , Zhao Andi , Wang Biyuan , Yang Jiao , Wang Le , Zhang Lingxiao , Dong Danfeng , Chen Ling , Yang Jin 
Published: August 2020

Abstract
Background and objectives
Malignant phyllodes tumor of the breast (MPTB) is a kind of rare tumor. Our objective was to investigate the role of adjuvant radiotherapy (RT) in MPTB patients.

Methods
MPTB patients were identified in the Surveillance, Epidemiology and End Results (SEER) database. Kaplan–Meier curves and multivariable Cox proportional hazards analyses were conducted to determine the effect of adjuvant RT on MPTB patients. Propensity-score matching (PSM) method was used to balance the clinicopathological characteristics.

Results
A total of 1353 MPTB patients were included in our study and the median follow-up time was 99 months (range: 0–331 months). 16.7% (226) MPTB patients received adjuvant RT, of which 49.1% (111) received mastectomy and 50.9% (115) underwent breast conservation surgery (BCS). Patients receiving adjuvant RT were more likely to be white, with better differentiation and larger tumors (p < 0.05). Multivariate analysis showed that poorer tumor differentiation grade, larger tumor size, and lymph node metastasis were associated with reduced survival while BCS was a protective factor of disease-specific survival (DSS) (HR 0.297; 95% CI 0.184–0.480) and overall survival (OS) (HR 0.445; 95% CI 0.321–0.616). After PSM, survival curves showed patients did not achieve an improved OS or DSS from adjuvant RT (p > 0.05). In subgroup analysis, no subgroup benefited from adjuvant RT. Exploratory analysis showed a survival benefit trend from adjuvant RT in patients with tumor larger than 50 mm and undergoing BCS.

Conclusions
Among MPTB patients, adjuvant RT did not improve OS or DSS. In patients with tumor larger than 50 mm and receiving BCS, a survival benefit trend from adjuvant RT existed.
Morse Index and Stability of the Planar N-vortex Problem
Authors: Hu Xijun , Portaluri Alessandro , Xing Qin 
Published: August 2020

AbstractThis paper concerns the investigation of the stability properties of relative equilibria which are rigidly rotating vortex configurations sometimes called vortex crystals, in the N-vortex problem. Such a configurations can be characterized as critical point of the Hamiltonian function restricted on the constant angular impulse hyper-surface in the phase space (topologically a pseudo-sphere whose coefficients are the circulation strengths of the vortices). Relative equilibria are generated by the circle action on the so-called shape pseudo-sphere (which generalize the standard shape sphere appearing in the study of the N-body problem). Inspired by the planar N-body problem, and after a geometrical and dynamical discussion of the problem, we investigate the relation intertwining the stability of relative equilibria and the inertia indices of the central configurations generating such equilibria. In the last section we applied our main results to some symmetric three and four vortices relative equilibria.