bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-on-Premise Venues, Associated Risk Behaviors, and Attitudes toward Venue-Based HIV Testing among Men Who Have Sex with Men in Lima, Perú
Authors: Lankowski Alexander , Sánchez Hugo , Hidalgo José , Cabello Robinson , Duerr Ann 
Published: February 2020

Abstract
Background. In Perú, HIV disproportionately affects men who have sex with men (MSM). Despite widespread access to treatment, the high rate of new HIV infections has remained unchanged over the last decade. Low knowledge of HIV status associated with late diagnosis is a key factor underlying the high HIV incidence observed in this setting, creating conditions for efficient onward transmission. Improving access to HIV testing and prevention services for those at highest risk is an important public health priority. Sex-on-premise venues (SOPVs) – saunas, sex clubs, pornographic movie theaters, hourly hotels, and bars/discos with areas where sex is permitted – may be opportune sites for outreach; however, further research on SOPVs and the populations who frequent them is needed to inform such efforts.
Methods . We conducted a cross-sectional online survey of adult MSM in Lima, Perú to evaluate patterns of SOPV attendance, associated sexual risk behaviors, and attitudes toward SOPV-based interventions. Participants were recruited through outreach to social media networks affiliated with local LGBTQ-aligned community groups. Our primary analytic objective was to estimate the association of HIV-related sexual risk behaviors and SOPV attendance. Additionally, we performed exploratory analyses to describe risk behavior stratified by SOPV category and to examine the relationship between SOPV attendance and the use of online platforms to meet sex partners.
Results. Overall, 389 MSM completed the survey from November 2018 through May 2019, of whom 68% reported attending an SOPV in the last three months. SOPV attendance was associated with multiple sexual risk behaviors, including transactional sex, group sex, substance use around the time of sex, and higher number of partners. Over two thirds of SOPV attendees indicated they would accept HIV testing if offered at SOPVs.
Conclusions. SOPV attendance was common among MSM in Lima who participated in our survey, and SOPV attendees reported significantly greater engagement in sexual risk behaviors related to HIV transmission. Attitudes toward hypothetical SOPV-based interventions were generally favorable. These findings suggest that outreach at SOPVs may be an effective mechanism for reaching a particularly high-risk sub-population of MSM in Perú to deliver targeted HIV testing and prevention interventions.
Implementation strategies in emergency management of children: a scoping review protocol
Authors: Aregbesola Alex , Abou-Setta Ahmed M. , Jeyaraman Maya M. , Okoli George , Lam Otto , Sibley Kathryn M. , Klassen Terry P. 
Published: February 2020

Abstract
Background : Behaviour change is not simple, and the introduction of guidelines or protocols does not mean that they will be followed. As such, implementation strategies are vital for the uptake and sustainability of changes in medical protocols. Medical or mental emergencies may be life threatening, especially in children due to their unique physiological needs. In emergency departments (EDs), where timely decisions are often made, practice change requires thoughtful considerations regarding the best approaches to implementation. As there are many studies reporting on a wide variety of implementation strategies in emergency management of children in EDs, we aim to identify and map the characteristics of these studies. Methods : We will conduct a scoping review to identify various implementation strategies in emergency management of children using the Arksey and O’Malley framework. We will search Medline (Ovid), Embase (Ovid), Cochrane Central (Wiley) and CINAHL (Ebsco), from inception through May 29, 2019, for implementation studies among the pediatric population (≤21 years) in a pediatric emergency setting. Two pairs of reviewers will independently select studies for inclusion and extract data. We will perform a descriptive, narrative analysis on the characteristics of the identified implementation strategies. Discussion : We will present specific characteristics and outcome measures of all included studies in a tabular form. The results of this review are expected to help identify and characterize successful implementation strategies in emergency management of children in EDs. Scoping review registration : Open Science Framework https://osf.io/h6jv2
Assessment of risk factors for early childhood caries at different ages in Shandong, China and reflections on oral health education: a cross-sectional study
Authors: Wang Zhifeng , Zhang Meng , Zhang Xinyue , Zhang Yuan , Li Yanan , Shao Chunchun , Xiong Shijiang , Lan Jing 
Published: February 2020

Abstract
Background: The high prevalence of early childhood caries (ECC) is widespread around the world, and oral health education (OHE) plays a vital role in preventing ECC. Numerous studies on ECC risk factor assessment have assisted us in enriching the content of OHE. The objective of this study was to further assess independent risk factors for ECC at different ages to provide evidence and insights for OHE.Methods: Children aged 3-5 years old (N=1301) in Shandong Province were enrolled in this cross-sectional study. Data about oral health status and guardians’ oral health knowledge, attitude, and practice (KAP) were extracted from the 4th National Oral Health Survey of China. The associations between ECC prevalence and various KAP variables were tested with chi-square tests, bivariate analysis and multivariable logistic regression analyses.Results: The ECC prevalence in Shandong Province was 64.6%, and the dmft mean was 3.15. The independent variables with an increased risk for ECC were age, feeding method within 6 months of birth, bedtime sugar frequency, experience of toothache over the past year and dental visits. Feeding methods primarily contributed to the high risk of the 3-year-old group, while sugar habits mainly contributed to that of the 5-year-old group. The association between brushing and ECC was not confirmed in this study, and some positive knowledge and attitude variables were unexpectedly positively correlated with a high risk of ECC. Conclusion: These data provide evidence to suggest that the ECC-related risk factors at different ages are inconsistent, which provides some insights for OHE. Specifically, in addition to promoting preventive dental visit, we should highlight the effects of feeding methods in the early stages of deciduous dentition and sugar habits in the late stages of deciduous dentition on ECC, as well as encourage supplemental training for food oral health practices.
Social marketing interventions to promote physical activity among 60 years and older: a systematic review of the literature
Authors: Goethals Luc , Barth Nathalie , Hupin David , Mulvey Michael S. , Roche Frederic , Gallopel-Morvan Karine , Bongue Bienvenu 
Published: February 2020

Abstract
Background: Falls are a significant source of morbidity in people aged 65 and over, affecting one in three people in this age group. The scientific evidence indicates that physical activity is the most effective method for preventing falls among seniors. Although public health professionals often use social marketing is to design and plan successful interventions, its use to promote physical activity and prevent falls among older people remains low. This article aims to provide a new systematic literature review of social marketing interventions promoting physical activity and targeting people aged 60 and over.
Methods: Following PRISMA guidelines, we searched for relevant articles in five primary databases using predefined search and inclusion criteria. Two independent coders analysed the selected articles to identify evidence of the seven social marketing benchmark criteria, defined by experts in the field as the common elements that contribute to social marketing success.
Results: The final review included twenty articles covering six interventions. Of the studies selected, two specifically targeted over 60-year-olds, whereas the others segmented the population into several age-based subcategories, including over 60-year-olds. Five interventions highlighted positive results for the participants with an increase in participation or an increase in physical activity level. None of the six interventions selected for this literature review implemented the entire social marketing approach.
Conclusion: Few published interventions mobilise the seven social marketing criteria. Further research is required to encourage uptake and inclusion in successful social marketing interventions to increase program effectiveness in this target population.
Heparinized Chitosan Stabilizes the Bioactivity of BMP-2 and Potentiates the Osteogenic Efficacy of Demineralized Bone Matrix
Authors: Kim Soyon , Fan Jiabing , Lee Chung-Sung , Chen Chen , Bubukina Ksenia , Lee Min 
Published: February 2020

Abstract
Background: Demineralized bone matrix (DBM), an allograft bone processed to better expose osteoinductive factors such as bone morphogenetic proteins (BMPs), is increasingly used for clinical bone repair. However, more extensive use of DBM is limited by its unpredictable osteoinductivity and low bone formation capacity. Commercial DBM products often employ polymeric carriers to enhance handling properties but such carriers generally do not possess bioactive functions. Heparin is a highly sulfated polysaccharide and is shown to form a stable complex with growth factors to enhance their bioactivities. In this study, a new heparinized synthetic carrier for DBM is developed based on photocrosslinking of methacrylated glycol chitosan and heparin conjugation. Results: Heparinized chitosan exerts protective effects on BMP bioactivity against physiological stressors related to bone fracture healing. It also enhances the potency of BMPs by inhibiting the activity of BMP antagonist, noggin. Moreover, heparinized chitosan is effective to deliver bone marrow stromal cells and DBM for enhanced osteogenesis by sequestering and localizing the cell-produced or DBM-released BMPs. Conclusions: This research suggests an essential approach of developing a new hydrogel carrier to stabilize the bioactivity of BMPs and improve the clinical efficacy of current bone graft therapeutics for accelerated bone repair.
Retracted Publications in the Biomedical Literature from 2012-2018: An Overview
Authors: Wang Tao , Xing Qin-Rui 
Published: February 2020

Abstract
Background: The number of retracted publications in biomedical literature has increased dramatically in recent years. Many of these retractions are due to a faked review process and a large percentage of the retractions come from Chinese authors. The purpose of the current study was to determine the characteristics of retracted articles from 2012 to 2018 using the Medline database. Methods: The Medline database was searched to identify retracted publications from the year 2012 through 2017. Reasons for retraction were collected through PubMed, the Google search engine, and journal websites. Relevant data was collected for each retracted article and included the time from publication to retraction, the study type, the journal impact factor, and the institute of origin. Trends in characteristics related to retraction were determined. Results: Data from 4,043 retracted studies were included in the analysis. The most common reasons for retraction were fraud/suspected fraud (993), error (911), plagiarism (554), duplicate publication (547), and invalid peer review (384). The largest number of retracted articles was from China. Other countries with a large number of retractions were USA, Japan, India, Iran, Korea, Germany, and Italy. A faked review process, was responsible for a large number of retractions from China and Iran and from several journals. Conclusions: Misconduct, including fraud/suspected fraud, duplicate publication, and plagiarism, was the major cause for publication retraction. A faked review process caused the largest number of retractions in some countries. China has become the top country of origin for retracted publications.
Identification of aberrantly methylated differentially expressed genes in melanoma
Authors: Han Wei , Shen Guo-liang 
Published: February 2020

Abstract
Background: Skin Cutaneous Melanoma (SKCM) is known as an aggressive malignant cancer, which could be directly derived from melanocytic nevi. However, the molecular mechanisms underlying malignant transformation of melanocytes and melanoma tumor progression still remain unclear. Increasing researches showed significant roles of epigenetic modifications, especially DNA methylation, in melanoma. This study focused on identification and analysis of methylation-regulated differentially expressed genes (MeDEGs) between melanocytic nevus and malignant melanoma in genome-wide profiles. Methods: The gene expression profiling datasets (GSE3189 and GSE114445) and gene methylation profiling datasets (GSE86355 and GSE120878) were downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) and differentially methylated genes (DMGs) were identified via GEO2R. MeDEGs were obtained by integrating the DEGs and DMGs. Then, functional enrichment analysis of MeDEGs were performed. STRING and Cytoscape were used to describe protein-protein interaction(PPI) network. Furthermore, survival analysis was implemented to select the prognostic hub genes. Finally, we conducted gene set enrichment analysis (GSEA) of hub genes. Results: We identified 237 hypomethylated, upregulated genes and 182 hypermethylated, downregulated genes. Hypomethylation-upregulated genes were enriched in biological processes of the oxidation-reduction process, cell proliferation, cell division, phosphorylation, extracellular matrix disassembly and protein sumoylation. Pathway enrichment showed selenocompound metabolism, small cell lung cancer and lysosome. Hypermethylation-downregulated genes were enriched in biological processes of positive regulation of transcription from RNA polymerase II promoter, cell adhesion, cell proliferation, positive regulation of transcription, DNA-templated and angiogenesis. The most significantly enriched pathways involved the transcriptional misregulation in cancer, circadian rhythm, tight junction, protein digestion and absorption and Hippo signaling pathway. After PPI establishment and survival analysis, seven prognostic hub genes were CKS2, DTL, KIF2C, KPNA2, MYBL2, TPX2 and FBL. Moreover, the most involved hallmarks obtained by GSEA were E2F targets, G2M checkpoint and mitotic spindle. Conclusions: Our study identified potential aberrantly methylated-differentially expressed genes participating in the process of malignant transformation from nevus to melanoma tissues based on comprehensive genomic profiles. Transcription profiles of CKS2, DTL, KIF2C, KPNA2, MYBL2, TPX2 and FBL provided clues of aberrantly methylation-based biomarkers, which might improve the development of precise medicine.
Upregulation of circ_0000069 stimulates cervical cancer development by enhancing proliferation, migration, invasion while inhibiting apoptosis through the miR-4429/ZIC2 axis
Authors: Yang Hongjuan , Li Xiangkun , Zhao Xinwei , Wang Chang , Qin Dongyan , Gao Dongmei , Jiao Jinwen 
Published: February 2020

Abstract
Background Cervical cancer (CC) is a common female cancer according to global cancer statistics. The current study was used to investigate the regulatory mechanism of circ_0000069 in CC. Methods The real-time quantitative polymerase chain reaction (RT-qPCR) was used to assess circ_0000069, miR-4429, and zinc finger protein of the cerebellum 2 (ZIC2) expression in CC tissues and cells. Kaplan-Meier analysis was performed in CC patients to analyze the relationship between survival time and circ_0000069 expression. The proliferation, apoptosis, cell cycle of CC cells were detected by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazol-3-ium bromide (MTT), colony formation, and flow cytometry analyses, respectively. In addition, western blot assay was employed to show expression levels of apoptosis/cell cycle-related proteins, as well as ZIC2. The migration and invasion of CC cells were assessed by transwell analysis. Possible target miRNAs of circ_0000069, along with the interaction between ZIC2 and miR-4429 were confirmed by pull-down and dual-luciferase reporter assays. Eventually, the functional role of circ_0000069 in vivo was clarified with the xenograft experiment in nude mice. Results Circ_0000069 was overexpressed in CC tissues and cells than controls. Furthermore, the silencing of circ_0000069 inhibited proliferation, migration, and invasion while included apoptosis and cell cycle arrest of CC cells, which was overturned by downregulation of miR-4429. Importantly, ZIC2 was a direct target of miR-4429 in CC cells, and we further confirmed that overexpression of miR-4429 suppressed CC progress by decreasing ZIC2 expression in CC cells. Surely, silencing of circ_0000069 inhibited tumorigenesis in vivo. Conclusion Our current results suggested that circ_0000069 exerted its tumorigenic roles by regulation of proliferation, apoptosis, cell cycle, migration, and invasion of CC cells, supporting that circ_0000069/miR-4429/ZIC2 axis may provide potential prognostic biomarkers for CC.
Ginsenoside Rb1 alleviates lipopolysaccharide-induced inflammatory injury by downregulating miR-222 in WI-38 cells
Authors: Wei Erhu , Fang Xiao , Jia Peisheng , Li Mingxia , Jin Peina , Li Fengyan , Wang Huaili , Gao Dan 
Published: February 2020

Abstract
Background Pneumonia is a serious respiratory tract infection disease in children, which threatens to the health or life of children patients. Ginsenoside Rb1 (Rb1) is a principle active ingredient extracted from the root of Panax notoginseng (Burk.) F.H.Chen with anti-inflammatory effect. Our study aimed to determine the effects and molecular mechanisms of Rb1 on lipopolysaccharide (LPS)-induced inflammatory injury of lung fibroblasts WI-38 cells. Methods Cell viability and apoptosis were evaluated by CCK-8 and flow cytometry analysis, respectively. The production of inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin (IL)-lβ, and IL-6 were measured by ELISA and RT-qPCR. miR-222 expression was examined by RT-qPCR. The expression levels of the nuclear factor-kappa B (NF-κB) p65 and phosphorylated p65 were detected by western blot. Results LPS stimulation induced WI-38 cell inflammatory injury by inhibiting cell viability, and inducing apoptosis and inflammatory cytokine production, while treatment with Rb1 significantly attenuated LPS-induced inflammatory injury in WI-38 cells. Additionally, Rb1 decreased LPS-induced upregulation of miR-222 and activation of the NF-κB pathway in WI-38 cells. Overexpression of miR-222 abolished the inhibitory effects of Rb1 on LPS-induced viability reduction, apoptosis, inflammatory cytokine production and activation of the NF-κB pathway. Conclusion Rb1 alleviated LPS-induced inflammatory injury in WI-38 cells via downregulating miR-222 and inactivation of the NF-κB pathway, contributing to our understanding of the effects and molecular mechanism of Rb1 in pneumonia.
Host DNA released by NETosis in neutrophils exposed to seasonal H1N1 and highly pathogenic H5N1 influenza viruses
Authors: Chan Louisa L.Y. , Nicholls John M. , Peiris J.S. Malik , Lau Yu Lung , Chan Michael C.W. , Chan Renee WY 
Published: February 2020

Abstract
Background Neutrophil (Nϕ) is of the most abundant number in human immune system. During acute influenza virus infection, Nϕs are already active in the early phase of inflammation-a time in which clinical biopsy or autopsy material is not readily available. However, the role of Nϕ in virus infection is not well understood. Here, we studied the role of Nϕ in host defense during influenza A virus infection, specifically assessing if it contributes to the differential pathogenesis in H5N1 disease. Methods Nϕs were freshly isolated from healthy volunteers and subjected to direct influenza H1N1 and H5N1 virus infection in vitro . The ability of the naïve Nϕs to infiltrate from the basolateral to the apical phase of the influenza virus infected alveolar epithelium was assessed. The viral replication, innate immune responses and Neutrophil extracellular trap (NET) formation of Nϕs upon influenza virus infection were evaluated. Results Our results demonstrated that influenza virus infected alveolar epithelium allowed more Nϕs transmigration. Significantly more Nϕs migrated across the H5N1 influenza virus infected the epithelium than the counterpart infected by the seasonal influenza H1N1 virus infected. Nϕs were equally susceptible to H5N1 and H1N1 virus infection with similar viral gene transcription. Productive replication was observed in H5N1 infected Nϕs. Both H5N1 and H1N1 infected Nϕs induced cytokines and chemokines including TNF-α, IFN-β, CXCL10, MIP-1α and IL-8. This inferred a more intense inflammatory response posed by H5N1 than H1N1 virus. Strikingly, NADPH oxidase-independent NET formation was observed in H1N1 infected Nϕs at 6 hpi while no NET formation was observed upon H5N1 infection. Conclusion Our data is the first to demonstrate that NET formation is abrogated in H5N1 influenza virus infection. Its contribution to the differential severity of H5N1 disease requires further investigation.
Influence of marital status on overall survival in adult chordoma patients: A SEER-based study
Authors: Tang Chao , Wang Ruiliang , Lu Qingguo , Wang Shantao , Jia Gen , Cao Pengfei , Nie Xinfa , Zhang Hailong 
Published: February 2020

Abstract
Background. As a rare primary bone tumor, no studies have reported the relationship between prognosis and marital status in patients with chordoma. Methods. We classified chordoma patients identified from the Surveillance, Epidemiology, and End Results (SEER) database from 1975 to 2016 into four groups: married, divorced/separated, widowed and single groups. Kaplan-Meier curves with log-rank test and Cox regression were used to analyse the effect of marital status on overall survival (OS). Results. A total of 1,080 patients were included in the study, 700 (64.8%) were married, 88 (8.1%) were divorced/separated, 78 (7.2%) were widowed and 214 (19.8%) were single. Among the four groups, the 5-year OS (45.2%), 10-year OS (12.5%) and median OS (56.0 months) were the lowest in the widowed group. After including age, sex, primary site, marital status, disease stage, tumor size, histological type, and treatment pattern, multivariate analysis showed that marital status was still an independent risk factor for chordoma patients, widowed patients have the lowest OS (hazard ratio [HR]: 1.71; 95% confidence interval [CI]: 1.25–2.33, p<0.001) compared with married patients. Similar results were observed after stratifying the primary site and disease stage. Conclusion. Marital status was an independent prognostic indicator for adult chordoma patients, and marital status was conducive to patient survival. Compared with married patients, widowed patients have a higher risk of death.