bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global miRNA Expression Analysis Identifies miR-194, miR-100, miR-125b and miR-199a as Promising Biomarkers for Gastric Cancer and miR-194 Inhibits Gastric Cancer Cell Growth by Targeting CCND1
Authors: Li Dandan , Wang Jingjie , Zhang Meixin , Hu Xinhui , She Jiajun , Cai Mengyu , Yang Lijuan , Liu Weijia , Hu Qiwen , Qiu Xuemei , Qin Shanshan 
Published: August 2020

Abstract
BackgroundDifferent gastric cancer (GC) subtypes usually possess distinct clinical outcomes. The function of miR-194 in gastric cancer remains unclear and controversial. This study aimed to identify potential microRNAs that differentially expressed in subtypes and to elucidate the molecular mechanisms of miR-194 in GC.MethodsComprehensive miRNA expression analysis was performed using the available miRNA-seq data from TCGA stomach cancer cohort. The preferences of miR-194 in regulating target genes were determined by RNA sequencing studies. The function of miR-194 in GC was explored in GC cell lines by performing qRT-PCR assays, western blot assays, cell proliferation assays, luciferase report assays and flow cytometry assay.ResultsIn this study, we identified a series of miRNAs that can serve as prognostic biomarkers for GC. Among them, miR-100, miR-125b, miR-199a and miR-194 were the 4 most promising prognostic biomarkers in GC due to their significant associations with various clinical characteristics of patients. MiR-100, miR-125b and miR-199a predicted poor prognosis in GC, while miR-194 predicted favorable prognosis in GC. Besides, we provided the first comprehensive transcriptome analysis about miR-194 in GC. The results showed that miR-194 tended to regulated target genes by binding on their 3' untranslated regions in a 7-mer-A1 or 7-mer-m8 or 8-mer manner. The KEGG pathway analysis showed that cell cycle was one of the most affected pathways by miR-194 in GC. Moreover, CCND1 was proved to be a novel target gene of miR-194 in GC. Additionally, the downregulation of CCND1 by miR-194 in GC further led to cell growth inhibition and cell cycle arrest. ConclusionsMiR-100, miR-125b, miR-199a and miR-194 could serve as prognostic and diagnostic biomarkers for GC. MiR-194 might suppress GC cell growth mainly through targeting CCND1 and induction of cell cycle arrest.
HTATIP2 Represents an Innovative Bio-marker in Hepatocellular Carcinoma Detection
Authors: Zhang Xianfeng , Zhang Xianjun , Li Xinguo , Bao Hongbing , Li Guang , Li Ning , Li Hengli , Dou Jian 
Published: August 2020

Abstract
Background: This study was designed to investigate the serum level of HIV-1 Tat interactive protein 2 (HTATIP2) mRNA in hepatocellular carcinoma (HCC) patients and its diagnostic significance in the disease.Methods: The serum HTATIP2 mRNA level was determined by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between HTATIP2 expression and clinical parameters was analyzed using Chi-square test. Receiver operating characteristics (ROC) curve was adopted to estimate the diagnostic role of serum HTATIP2 in HCC.Results: HCC patients showed a significantly lower serum level of HTATIP2 than the healthy control (P<0.001). The level of HTATIP2 was closely associated with venous invasion (P=0.011), lymph node metastasis (P=0.007) and TNM stage (P=0.016). ROC curve demonstrated that HTATIP2 could discriminate between HCC patients and healthy individuals at the optimal cutoff point of 2.39. Besides, the AUC was 0.892, with the corresponding sensitivity and specificity of 83.90% and 84.37%, respectively. Conclusions: HTATIP2 is negatively expressed in HCC and may be a diagnostic biomarker for this disease.
CCDC12 Promotes Tumor Development and Invasion Through the Snail Pathway in Colon Adenocarcinoma
Authors: Du Fengying , Li Leping , Wang Qiang , Pei Wenting , Zhuo Hongqing , Peng Lipan , Xu Tao , Jing Changqing , Zhang Jizhun 
Published: August 2020

Abstract
Background: To determine the role of Coiled-coil domain containing 12 (CCDC12) in colon adenocarcinoma (COAD).Methods: We used integrative expression Quantitative Trait Loci (eQTL) analysis to identify risk single nucleotide polymorphisms (SNPs) and associated genes. Immunohistochemical staining (IHC) and western blotting (WB) were used to detect CCDC12 expression. We selected SW480 and LOVO cell lines to knock down CCDC12, while HCT116 and SW480-KD cell lines were up-regulated. Then performed functional studies and established a xenograft tumor model in BALB/c mice. Four plex Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) assays were performed to determine its function and potential regulatory mechanism. Overexpressed Snail and knocked down CCDC12 subsequently in SW480 cells to find them association.Results: Integrative eQTL analysis found that rs8180040 was significantly associated with CCDC12 in COAD patients. IHC and WB confirmed CCDC12 was highly expressed in COAD tissues (IHC 51/75 vs 8/75, WB 10/12 vs 2/12). This was consistent with RNA-Seq data from TCGA database (P value < 0.001). Knockdown of CCDC12 could significantly reduce proliferation, migration, invasion and tumorigenicity of colon cancer cells, while exogenous overexpression of CCDC12 had the opposite effect. iTRAQ assays demonstrated that overexpression of CCDC12 would change proteins on adherens junction pathway. Overexpression of Snail did not significantly change CCDC12 levels in SW480 cells, while knock down of CCDC12 reduced that of Snail.Conclusions: CCDC12 plays a significant role in tumorigenesis, development and invasion of COAD and may affect the epithelial to mesenchymal transformation process of colon cancer cells by regulating the Snail pathway.
Association Between ABCA1 (R219K) Polymorphism and Lipid Profiles: A Meta-Analysis
Authors: Shi Zhangyan , Tian Yajie , Wu Yufei , Li Junlin , Chen Qianliang , Zhang Kejin 
Published: August 2020

Abstract
Background
Conflicting evidence was found about the relationship between lipid profiles and R219K in adenosine triphosphate-binding cassette exporter A1 (ABCA1) gene. A meta-analysis was conducted to assess the effect of R219K on lipid level.
Methods
Multi-database literature search was performed in accordance with PRISMA guidelines. The effect of each study was expressed by standard mean difference (SMD) and 95% confidence interval and pooled by meta-analysis in a random-effects model. Sub-group and meta-regression analyses were also conducted to explore potential heterogeneity sources.
Results
Overall pooled effect showed that individuals with RR genotype had significantly lower HDLC level (SMD = -0.17 mmol/L, 95% CI: -0.22 ~ -0.12, z = -6.39, P < 0.001) than those with K allele. However, this effect mainly presented in Asians (SMD = -0.24 mmol/L, 95% CI: -0.32 ~ -0.17, z = 6.60, P < 0.001) rather than Caucasian populations, and the difference was significant (Q = 5.20, df = 1, P = 0.02). The relationship between R219K polymorphism and LDLC, TC, and TG levels was not determined.
Conclusions
R219K polymorphism in the ABCA1 gene was associated with HDLC level in Asian populations in the present meta-analysis. This correlation was not substantially affected by the age, gender or health condition of individuals.
THOTTIP is Associated with Prognosis of Lung Cancer in the Chinese Population
Authors: Zhao Yue , Kong Xiangjun , Wang Hongbing 
Published: August 2020

Abstract
Background: Lung cancer is a prevent malignancy with high mortality. Long noncoding RNAs (lncRNAs) have been reported to play important roles in tumorigenesis. The purpose of this study was to explore the prognostic value of lncRNA HOTTIP in lung cancer.Methods: The expression of HOTTIP in lung cancer tissues was measured by quantitative real-time PCR (qRT-PCR). Chi-square test was applied to assess the correlation of HOTTIP with clinicopathological features. Overall survival curve was built by Kaplan-Meier method with log rank test. Cox regression analysis was used to explore the prognostic value of HOTTIP in lung cancer.Results: The expression of HOTTIP was significantly increased in lung cancer samples compared with paired noncancerous samples (P<0.001). Moreover, its expression patterns were correlated with lymph node metastasis (P=0.039) and TNM stage (P=0.007). Survival curve demonstrated that lung cancer patients with high level of HOTTIP had poor survival rate (log-rank P=0.011). HOTTIP might be an independent prognostic factor for lung cancer (HR=1.916, 95%CI=1.133-3.238, P=0.015).Conclusions: HOTTIP is up-regulated in lung cancer, and associated with aggressive tumor progression. HOTTIP may be a potential prognostic biomarker for lung cancer.
Should we Investigate Mitochondrial Disorders in Progressive Adult-onset Undetermined Ataxias?
Authors: Pedroso José Luiz , Pinto Wladimir B Rezende , Barsottini Orlando G , Oliveira Acary S B 
Published: August 2020

Abstract
Background: Despite the broad development of next-generation sequencing approaches recently, such as whole-exome sequencing, diagnostic workup of adult-onset progressive cerebellar ataxias without remarkable family history and with negative genetic panel testing for SCAs remains a complex and expensive clinical challenge. Case presentation: In this article, we report a Brazilian man with adult-onset slowly progressive pure cerebellar ataxia, which developed neuropathy and hearing loss after fifteen years of ataxia onset, in which a primary mitochondrial DNA defect (MERRF syndrome - myoclonus epilepsy with ragged-red fibers) was confirmed through muscle biopsy evaluation and whole-exome sequencing. Conclusions: Mitochondrial disorders are a clinically and genetically complex and heterogenous group of metabolic diseases, resulting from pathogenic variants in the mitochondrial DNA or nuclear DNA. In our case, a correlation with histopathological changes identified on muscle biopsy helped to clarify the definitive diagnosis. Moreover, in neurodegenerative and neurogenetic disorders, some symptoms may be evinced later during disease course. We suggest that late-onset and adult pure undetermined ataxias should be considered and investigated for mitochondrial disorders, particularly MERRF syndrome and other primary mitochondrial DNA defects, together with other more commonly known nuclear genes.
Assessing Socioeconomic Opportunities of Women Selling Injera on Street at Nekemte Town, Oromia, Ethiopia
Authors: Ebissa Tarekegn Tariku 
Published: August 2020

Abstract
Injera is the most preferableand popular type of feeding items in Ethiopia. Injera can be prepared from various flours of cereal grains but it mostly produced using grinded flour of cereal grain called Teff (Eragrostistef (Zucc) Trotter). This study is conducted to assess the socioeconomic opportunities of women selling Injera on the street in Nekemtetown, Oromia, Ethiopia. The main aim of the study was indentifying and analyzing the social and economic opportunities of women engaged on selling Injera on the street. Primary data was collected from 50 women currently selling Injera on street at Nekemte town through structured interview questionnaire. The interview was conducted within one month at five Injera marketing locations in the town. Simple descriptive statistic was used to analyze the collected data. The findings of the study reveal the existence of both social and economic opportunities. The significant opportunities are the existence of indigenous knowledge and skill, good future employment option, availability of high domestic & abroad market demand, existence of high interest to work in cooperatives, existence of necessary inputs in the location, availability of cheap facilities and utilities to support the engagement and existence of eager interest to continue in supplying and trading Injera. As a result, for wise utilization of the opportunities, it is recommended to empower women engaged on selling Injera by enterprising and modernizing the production, supply and trading of Injera in Nekemte town.
CT Chest at discharge in Hospitalized Covid-19 patients: Should we worry about the long term evolution of parenchymal lesions?
Authors: Pauline Pradere , Olaf Mercier , Gaelle Dauriat , Jerome Le Pavec , Sebastien Hascoet , Anne Sigal-Cinqulabre , Olivier Planche , Marc Zins , Caroline Caramella 
Published: August 2020

Abstract
Objectives: Covid-19 epidemic has led to thousands of hospitalized patients and the fear of long-term pulmonary sequelae is real. This preliminary study aimed at describing the pattern of lung parenchymal lesions in patients at the time of clinical recovery. Methods Patients who were hospitalized for a severe Covid-19 pneumonia and who underwent a CTchest less at the time of discharge were included. CT scan parenchymal lesions were classified using international recommendations and compared to the diagnostic CT scan. Results We included 32 patients, median age 57 yo [26-89]. Out of them, 10 patients required ICU admission. The median hospital stay was 12 days [4-28]. All CT chest at discharge showed persistent parenchymal abnormalities regardless of the time of clinical recovery or ICU requirement. The main radiological pattern at admission was bilateral ground glass opacities in 28/30 (93.3%), associated in 12 patients (40%) with areas of consolidation, and organized pneumonia in 8 patients (27%). At discharge, the main radiological pattern remained bilateral ground glass opacities in 29/32 patients (91%) associated with consolidation in 3/32 patients (9%) and organized pneumonia in 25/32 patients (78%). There was no correlation between lesions extent and clinical severity, particularly ICU requirement. Conclusion CT-chest of patients recovering from severe covid-19 show parenchymal persistent abnormalities: careful consideration of the organisation of recovery of lung function follow-up is thus needed and the question of antifibrotic agent usefulness may be anticipated.Conclusion CT-chest of patients recovering from severe covid-19 show parenchymal persistent abnormalities : careful consideration of the organisation of recovery of lung function follow-up is thus needed and the question of antifibrotic agent usefulness may be anticipated.
Establishment and Validation of a Predictive Model for Non-tuberculous Mycobacterial Infections in Acid-fast Bacilli Smear-positive Patients
Authors: Chen Xianqiu , Zhang Yuan , Xu Jinfu , Li Huiping 
Published: August 2020

Abstract
Background: To identify non-tuberculous mycobacteria (NTM) infections in initially acid-fast bacilli (AFB) smear-positive patients more quickly and effectively, we attempted to establish a predictive model and validate it in clinical practice.Methods: A total of 125 AFB smear-positive patients with mycobacteriosis diagnosed in the Respiratory Department of Shanghai Pulmonary Hospital were retrospectively analyzed，including 64 cases of NTM and 61 cases of pulmonary tuberculosis (PTB), to identify the clinical features distinguishing NTM from PTB patients. A bivariate regression was then set up to determine the independent predictive risk factors to NTM infections. A receiver operating characteristic (ROC) curve was used to determine the model’s predictive discrimination. Finally, the model was verified both internally and externally for its predictive ability.Results: Compared with PTB, female patient, the symptom of hemoptysis, the exists of bronchiectasis, and negative test for QuantiFERON tuberculosis (QFT) were more common in NTM patients (78.1% vs. 34.4%, 37.5% vs. 11.5%, 76.6% vs. 19.7%, 82.8% vs. 8.2%, respectively, P≤0.001). The lesions on chest high-resolution computed tomography (HRCT) in NTM patients were more commonly involved in the right middle lobe (87.5% vs. 52.5%, P<0.001) and left lingular lobe (71.9% vs. 50.8%, P=0.016), and cystic change was more commonly morphological alteration (32.8% vs. 8.2%, P=0.001). Binary regression analysis showed that female patient, the exists of bronchiectasis, negative test for QFT and right middle lobe lesion were independent risk factors for NTM in AFB smear-positive patients (P<0.05). A ROC curve combining the four risk factors showed a sensitivity and specificity of 85.9% and 93.4%, respectively, and the area under the curve (AUC) was 0.963. Moreover, internal validation and external validation of the later clinical data both confirmed the effectiveness of the model.Conclusions: The predictive model would be useful for early differential diagnosis of NTM in initially AFB smear-positive patients.
Prediction Model for Breastfeeding practice among Ethiopian Children using Decision tree and Rule induction Algorithms : Data mining
Authors: Awalom Taddele Weldeslassie , Adem Mesaud Mohamodbrhan , Araya Mearg 
Published: August 2020

Abstract
Background: Ethiopia have adopted infant feeding guidelines based on World Health Organization's standards to reduce the burden of infant and child mortality due to poor breastfeeding practice. But, breastfeeding practice is still one of challenges affecting infants and child health causing significant amount of deaths(23%-28%) yearly in Ethiopia. Breastfeeding practice is associated with different individual and community specific socio-cultural factors in different countries. Ethiopia is a populated country of communities with a very diverse cultural and societal values administered in nine different regions. Therefore, it is very important to assess breastfeeding practice among the various communities to identify the factors at individual and community level in order to come up with preventive intervention protocols that matches to each particular region. Hence, the study intended to assess patterns of breastfeeding practice among the communities within each specific region and develop predictive model of breastfeeding practice using data mining algorithms in Ethiopia. Different experiments were conducted in four scenarios with two test option (10 cross validation and percentage splits) and different parameter values using J48 and PART algorithms to select best predictive model for developing breastfeeding decision support system using java application programming interface. Results: About 54.8% (6390) and 3.8% (445) of the mothers have ever and never breastfed their children within the previous five years of the survey respectively while 40.8% (4757) mothers were breastfeeding until the survey date. Both J48 and PART algorithms were able to predict breastfeeding practice with an accuracy of 96.86% and 96.77% respectively. 2316 (96.94%) and 1071 (96.04%) mothers were correctly classified as Normal and poor respectively using PART algorithm with 70-30 percentage-split test option. Only 66 (3.06%) and 43 (3.96%) mothers were misclassified as false positive and false negative respectively.Conclusions: Almost, half of the mothers with 1-4 births within the five years before the survey have had normal breastfeeding practice. Both J48 and PART algorithms have best fitted to predict breastfeeding practice and can be used to deploy a decision support model of breastfeeding practice as a supporting tool for health practitioners.
Elective Surgical Case Cancellation and Reasons in Ethiopia: A Systematic Review and Meta-Analysis
Authors: Yohanes Yeneabat Birhanu , Sinshaw Aklilu Endalamaw , Ayisa Aynalem Adu 
Published: August 2020

Abstract
Background: Cancellation of elective surgical operation recognized as a major cause of emotional trauma to patients as well as their families. In Ethiopia, prevalence and reasons for elective surgical case cancellation are variable from time to time in different settings. This systematic review and meta-analysis aimed to find the pooled prevalence of elective surgical case cancellation. Besides, it aimed to find reasons for elective surgical case cancellation in Ethiopia.Methods: The databases for the search were Web of Science, PubMed, and Google Scholar by the date 02/08/2020. To assess publication bias Egger's test regression analysis was applied. Results: This meta-analysis included a total of 5 studies with 5591 study participants. The pooled prevalence of elective surgical case cancellation was 21.41% (95% CI: 12.75 to 30.06%).Administration-related (34.50%) was the most common identified reason. Followed by Surgeon (25.29%), Medical (13.90%), and Patient-related reasons (13.34%).Conclusions: The national prevalence of elective surgical case cancellation was considerable. The most common reason for elective surgical case cancellation was administration-related reasons, followed by the surgeon, medical and patient-related reasons. The administrative or management-related reasons for the cancellations are potentially preventable. Thus, efforts should be made to prevent unnecessary cancellations through careful planning.
Unusual Pediatric Sigmoid Perivascular Epithelioid Cell Tumor with Regional Lymph Node Metastasis Treated Using Gemcitabine and Docetaxel: A Case Report and Literature Review
Authors: Cheng Hsiu-Chung , Kuo Chia-Yu , Huang Ching-Wen , Shih Hsiang-Hung , Lin Chih-Hung , Wang Jaw Yuan 
Published: August 2020

Abstract
Background Perivascular epithelioid cell tumor (PEComa) is an extremely rare neoplasm with distinctive morphology and specific expression of immunohistochemical markers. The lesion is typically diagnosed in middle-aged women, and few pediatric cases have been reported. However, standardized treatment for the tumor type remains unestablished. Herein, we report a case of a pediatric patient with gastrointestinal PEComa of the sigmoid colon with regional lymph node metastasis. The patient was successfully treated with complete tumor resection and gemcitabine/docetaxel combination chemotherapy without local or distant recurrence after 12 months postsurgery.Case presentation A 17-year-old female adolescent presented with gastrointestinal PEComa of the sigmoid colon with regional lymph node metastasis. She was treated with surgical resection of the tumor and cytotoxic chemotherapy with gemcitabine (900 mg/m2) and docetaxel (100 mg/m2) every 3 weeks. There was no recurrence of hematochezia stool, and complete response was achieved, with a progression-free survival of 12 months.Conclusion Surgical resection with adjuvant conventional cytotoxic chemotherapy can be considered as the first-line treatment for early-stage gastrointestinal PEComa.
Knockdown of POLQ Interferes the Development and Progression of Hepatocellular Carcinoma Through Regulating Cell Proliferation, Apoptosis and Migration
Authors: Pan Qi , Wang Lu , Liu Yu , Li Min , Zhang Yao , Peng Wei , Deng Tan , Peng Mei-ling , Jiang Jin-qiong , Tang Jiao , Duan Hua-xin , Fan Sha-sha 
Published: August 2020

Abstract
Background: DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs), whose function in hepatocellular carcinoma has not been investigated.Methods: In the present study, both the data collected from the Cancer Genome Atlas (TCGA) and our group’s results showed higher POLQ expression in HCC tissues than the para-cancerous tissues, which was associated with higher malignancy and poor prognosis. POLQ knockdown HCC cell model (shPOLQ) was constructed along with the corresponding negative control (shCtrl) through lentivirus infection for loss-of-function study. Results: We found that, upon knockdown of POLQ, the proliferation and migration of HCC cells decreased and apoptosis percentage increased. Moreover, the percentage of cells in G2 phase significantly increased in shPOLQ group compared with shCtrl group. Xenografts in mice grafted with shPOLQ cells grew much slower than that transplanted with shCtrl cells, and expressed lower Ki67 level. Furthermore, an apoptosis-related signaling array was used to explore the involvement of downstream signaling pathways, suggesting the enhanced phosphorylation of HSP27 and JNK, and the de-activation of mTOR, PRAS40, ERK1/2 and STAT3 pathways.Conclusions: Collectively, our study revealed that POLQ may participate in the development of HCC, depletion of which may be a promising treatment strategy for HCC.