bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of Socioeconomic Status on Preference for Sustainable Luxury Brands
Authors: Kim Jungkeun , Septianto Felix , Park Jooyoung 
Published: September 2020

Abstract
Sustainability has become an increasing concern for many brands and companies and an increasing number of luxury brands now engage in sustainability practices. The present research examines the factors influencing the effectiveness of embedding sustainability in luxury brands. Specifically, this paper investigates how childhood socioeconomic status (SES) moderates consumer preferences for sustainable (vs. regular non-sustainable) luxury brands. Across three experimental studies with different product categories and luxury brands, this paper finds that preferences for regular (vs. sustainable) luxury goods are stronger in people with a relatively high (vs. low) childhood SES (Studies 1-3). Notably, these preference patterns are driven by differences in the perceived quality of the brand among consumers with low versus high childhood SES (Study 2). However, these divergent patterns are attenuated when consumers experience high perceived environmental threat from the COVID-19 pandemic (Study 3). Taken together, these findings provide several theoretical and managerial implications.
Serine Proteases Profiles of Leishmania (Viannia) Braziliensis Clinical Isolates With Distinct Susceptibilities to Antimony
Authors: Zabala-Peñafiel Anabel , Dias-Lopes Geovane , Cysne-Finkelstein Léa , Conceição-Silva Fátima , Miranda Luciana de Freitas Campos , Fagundes Aline , Schubach Armando de Oliveira , Pimentel Maria Ines Fernandes , Souza-Silva Franklin , Machado Lucas de Almeida , Alves Carlos Roberto 
Published: September 2020

Abstract
Background: Glucantime® (SbV) is considered the first-line treatment against American Tegumentary Leishmaniasis in South America, though increased parasite resistance towards it have been reported and hampered its effectiveness. In this context, subtilisins serine proteases have been related to parasites’ susceptibility to drugs such as SbV and derivatives, through modulation of the parasite detoxification system. However, little is known about parasites causing ATL and their distinct responses towards this treatment.Methods: The study was conducted using Leishmania (Viannia) braziliensis clinical isolates from patients that presented clinical cure or Responders (R) and relapse/therapeutic failure or Non-responders (NR). Twelve clinical isolates were used to assess their in vitro susceptibility to SbIII and SbV, serine proteases activity, and expression of subtilisins-like and tryparedoxin-peroxidase (TXNPx) transcripts. Results: SbIII was able to better distinguish axenic amastigotes from each clinical group. These isolates were also assessed for serine protease activity, using z-FR-AMC as substrate and detecting distinct enzyme profiles with specific inhibitors. TLCK inhibited almost 100% of activity in both promastigotes and axenic amastigotes while AEBSF inhibited around 70%. PMSF showed low inhibition of specific isolates (35%). Gathering all the quantitative data, we performed principal component analysis and then used the K-means algorithm to cluster the isolates. This analysis yielded one cluster with only one isolate (R isolate), one homogeneous cluster (NR isolates), and three heterogeneous clusters (R and NR isolates). Additionally, gene transcripts of subtilisins and TXNPx were detected in promastigotes and axenic amastigotes from both groups. Conclusions: Cluster analysis showed that there is a phenotypic heterogeneity among the isolates, however, exploration of in vitro phenotypes based on SbIII and serine proteases profiles can aid in the characterization and better understanding of L. (V.) braziliensis clinical isolates.
Prediction of Overall Survival for Early-stage Non-small Cell Lung Cancer via a Novel 7-gene Prognostic Signature and Allele Frequency Deviation (AFD)
Authors: AL-Dherasi Aisha , Liao Yuwei , Huang Qi-Tian , Wang Yichen , Hua Rulin , Mosiab Sultan AL , Zhang Jingkai , Yu Ying , Zhang Yu , Zhang Xuehong , Huang Chao , Sufiyan Sufiyan , Ge Dongcen , Bai Wanting , Liu Ruimei , Shao Yanyan , Li Yulong , Mousa Haithm , Shi Leming , Lv Dekang , Li Zhiguang , Liu Quentin 
Published: September 2020

Abstract
Background: Due to the late and poor prognosis of non-small lung cancer(NSCLC), the mortality of patients is high, underlines the need to identify a credible prognostic marker for NSCLC patients. The aim of our study is to examine the association of allele frequency deviation (AFD) with the patient's survival, as well as identification and validation of a new prognostic signature to predict NSCLC overall survival(OS).Methods: First, we developed a new algorithm to calculate AFD from whole-exome sequencing(WES) data, then we compared the predictability of the patient's survival between AFD, tumor mutation burden (TMB) and change of variants allele frequency (dVAF). Second, we overlapped the differentially expressed genes (DEGs) from our data with the genes associated with the survival of The Cancer Genome Atlas (TCGA) database to confirm all genes significantly related to the survival of lung cancer. We identified 149 genes, 31 of which are new genes and have not been reported for lung cancer, that was used to develop a new prognostic model. Lung cancer adenocarcinoma (LUAD) data from the TCGA database was used to validate the gene-signature model. The prognostic model relating to the genes was established and validated in training and LUAD validation groups. Results: There was a significant association found between the high AFD value and poor survival among non-small cell lung cancer (NSCLC) patients. A novel seven genes (UCN2, RIMS2, CAVIN2, GRIA1, PKHD1L1, PGM5, CLIC6) were obtained through multivariate Cox regression analysis and significantly associated with NSCLC patients survival. Cox regression analysis confirmed that AFD and 7-gene signature are an independent prognostic marker in NSCLC patients. The AUC for 5-year survival in AFD and the AUC for 3-year survival in both training and validation groups were greater than 0.7.Conclusion: As a result, AFD and 7-gene signatures were identified as new independent predictive factors used for predicting the survival among NSCLC patients.
Identification of Autophagy-related Long Non-coding RNA Prognostic Signature for Clear Cell Renal Cell Carcinoma
Authors: Cui Yankang , Miao Chenkui , Liang Chao , Chen Xiaochao , Yan Tao , Bu Hengtao , Dong Huiyu , Li Junchen , Zhang Shaobo , Li Jie , Wang Zengjun , Liu Bianjiang 
Published: September 2020

Abstract
BackgroundStudies over the past decade have shown that long non-coding RNAs (lncRNAs) play an essential role in the tumorigenesis and progression of kidney renal clear cell carcinoma (KIRC). Meanwhile, autophagy has been demonstrated to regulate KIRC pathogenesis and targeting therapy resistance. However, the prognostic value of autophagy-related lncRNAs in KIRC patients has not been reported before.MethodsIn this study, we obtained transcriptome data of 611 KIRC cases from the TCGA database and 258 autophagy-related mRNAs from the HADb database to identify autophagy-related lncRNAs by co-expression network. A prognostic model was then established basing on these autophagy-related lncRNAs, dividing patients into high-risk and low-risk groups. Survival analysis, clinical variables dependent receiver operating characteristic (ROC) analyses, univariate/multivariate Cox analyses, and clinical correlation analysis were performed based on risk signature with R language. Gene set enrichment analysis (GSEA) was then performed to investigate the potential mechanism of the risk signature promoting KIRC progression with GSEA software. ResultsA total of 17 lncRNAs were screened out and all these lncRNAs were found significantly related to KIRC patients' overall survival in subsequent survival analyses. Besides, the overall survival time in the high-risk group was much poorer than in the low-risk group. The ROC analysis revealed that the prognostic value of risk signature was better than age, gender, grade, and N stage. Univariate/multivariate analyses suggested that the risk signature was an independent predictive factor for KIRC patients. Immune-related pathways were dramatically enriched in high-risk patients according to GSEA. ConclusionsIn summary, our identified 17 autophagy-related lncRNAs had prognostic value for KIRC patients which may function in Immunomodulation.
Clinical Outcomes of Pediatric Macular Edema Associated with Non-infectious Uveitis
Authors: Nguyen Anh Hong , Mekonnen Bethlehem , Kim Eric , Acharya Nisha R. 
Published: September 2020

Abstract
Background Macular edema (ME) is the most frequent cause of irreversible visual impairment in patients with uveitis. To date, little data exists about the clinical course of ME in pediatric patients. A retrospective, observational study was performed to examine the visual and macular thickness outcomes of ME associated with chronic, noninfectious uveitis in pediatric patients.Methods Pediatric patients with noninfectious uveitis complicated by ME seen in the University of California San Francisco Health System from 2012-2018 were identified using ICD-9 and ICD-10 codes. Data were collected from medical records including demographics, diagnoses, ocular history, OCT imaging findings, complications, and treatments at first encounter and at 3, 6, 9, and 12-month follow-up visits. Cox proportional hazards regression was used to investigate the association between different classes of treatment (steroid drops, steroid injections, oral steroids and other immunosuppressive therapies) and resolution of macular edema. Results The cohort comprised of 21 children (26 eyes) with a mean age of 10.5 years (SD 3.3). Undifferentiated uveitis was the most common diagnosis, affecting 19 eyes (73.1%). The majority of observed macular edema was unilateral (16 patients, 76.2%) and 5 patients had bilateral macular edema. The mean duration of follow-up at UCSF was 35.3 months (SD 25.7).By 12 months, 18 eyes (69.2%) had achieved resolution of ME. The median time to resolution was 3 months (IQR 3-6 months). Median best-corrected visual acuity (BCVA) at baseline was 0.54 logMAR (Snellen 20/69, IQR 20/40 to 20/200). Median BCVA at 12 months was 0.1 logMAR (Snellen 20/25, IQR 20/20 to 20/50) Corticosteroid injections were associated with a 4.0-fold higher rate of macular edema resolution (95% CI 1.3-12.2, P = 0.01).Conclusions Although only 15% of the pediatric patients with uveitis in the study cohort had ME, it is clinically important to conduct OCTs to detect ME in this population. Treatment resulted in 69% of eyes achieving resolution of ME by 12 months, accompanied with improvement in visual acuity. Corticosteroid injections were significantly associated with resolution of macular edema.
miR-320a/SP1 negative reciprocal interaction contributes to cell growth and invasion in colorectal cancer
Authors: Zhang Wenjing , Yang Haitao , Wang Zhongqiu , Wu Yanting , Wang Jingzhai , Duan Guihua , Guo Qiang , Zhang Yu 
Published: September 2020

Abstract
Background: It has demonstrated that transcription factors (TFs) could be engaged reciprocal regulative circuits with some miRNAs to maintain the cellular homeostasis. Disequilibrium of the reciprocities by certain tumor-related stimuli, may give rise to deregulation of downstream cellular signaling pathways, thus promoting malignant phenotypes in tumor.Methods: We performed bioinformatics analysis, quantitative polymerase chain reaction (qRT-PCR), immunoblotting, dual-luciferase reporter assay, and a series of functional assays in vitro and in vivo, to describe a novel SP1/miR-320a reciprocal interaction in colorectal cancer (CRC).Results: Firstly, we found that miR-320a was statistically downregulated in CRC tissues and cell lines. Consistent with findings in other cancers, miR-320a exhibited inhibitory effects on cell growth and invasion of CRC in vitro and in vivo. Moreover, we identified Specificity Protein 1 (SP1), a well-known transcription factor, as a target gene of miR-320a, and ectopic SP1 expression partly abolished miR-320a-induced inhibitory effects. Reversely, we confirmed SP1 to interact with miR-320a promoter thus leading to depression of miR-320a. It hence illustrated a double-negative feedback loop engaging miR-320a with SP1. Additionally, on the basis that SP1 promoted MACC1 transcription, we by immunoblotting assay dissect that, the oncogenic signaling MACC1/MET was inactivated in the context of miR-320a-induced SP1 downregulation. Conclusion: Taken together, our study newly describes the miR-320a/SP1 negative reciprocal interaction contributes to cell growth and invasion in CRC, through modulating MACC1/MET signaling pathway.
Signatures of Landscape and Captivity in the Gut Microbiota of Southern Hairy-nosed Wombats (Lasiorhinus Latifrons)
Authors: Eisenhofer Raphael , Helgen Kristofer M , Taggart David 
Published: September 2020

Abstract
BackgroundHerbivorous mammals co-opt microbes to derive energy and nutrients from diets that are recalcitrant to host enzymes. Recent research has found that captive management—an important conservation tool for many species—can alter the gut microbiota of mammals. Such changes could negatively impact the ability of herbivorous mammals to derive energy from their native diets, and ultimately reduce host fitness. To date, nothing is known of how captivity influences the gut microbiota of the Southern Hairy-nosed Wombat (SHNW), a large herbivorous marsupial that inhabits South Australia. Here, using 16S rRNA gene sequencing, we characterized the faecal microbiota of SHNWs in captivity and from three wild populations, two from degraded habitats and one from an intact native grass habitat.ResultsWe found that captive SHNWs had gut microbiota that were compositionally different and less diverse compared to wild SHNWs. There were major differences in gut microbiota community membership between captive and wild, both in statistically significant changes in relative abundance of microbes, and in the presence/absence of microbes. We also observed differences in microbial composition between the wild populations, with the largest difference associated with native vs. degraded habitat. ConclusionsThese results suggest that captivity has a major impact on the gut microbiota of SHNWs, and that different wild populations harbour distinct microbial compositions. Such findings warrant further work to determine what impacts these changes have on the fitness of SHNWs, and whether they could be manipulated to improve future management of the species.
Evidence of an interaction between jets and intra-cluster magnetic layer
Authors: Chibueze James , Sakemi Haruka , Ohmura Takumi , Machida Mami , Akamatsu Hiroki , Akahori Takuya , Nakanishi Hiroyuki , Parekh Viral , Rooyen Ruby van , Takeuchi Tsutomu 
Published: September 2020

Abstract
Galaxy clusters are known to harbor magnetic field, however, the extent of the influence of the intra-cluster magnetic field on the cluster member galaxies remains an unresolved question. Intra-cluster magnetic field can be observed as density contact discontinuity formed by cool and dense plasma running into hot ambient plasma, and this exist in the central region of a merging galaxy cluster Abell 3376. Here we report on unambiguous evidence of an interaction between the relativistic electrons and intra-cluster magnetic fields from MeerKAT observations of a radio galaxy MRC0600-399, having bent jets. Contrary to typical bent jets, the jet shows a 90 degrees bend at the contact discontinuity and the collimated jet further extends over 100 kpc from the bend point. The spectral index flattens downstream of the bend point, indicating cosmic-ray re-acceleration. High-resolution numerical simulations reveal that the ordered magnetic field along the discontinuity, at which the intra-cluster magnetic field can be compressed and amplified, plays a significant role to the change in the direction of the jet propagation. The overall morphology of the bent jet bears remarkable similarities with the simulations, which greatly strengthens our understanding of the interaction between relativistic electrons and intra-cluster magnetic field.
Data-Driven Approach for Tailoring Facilitation Strategies to Overcome Implementation Barriers in Community Pharmacy
Authors: Moussa Lydia , Benrimoj Shalom I , Musial Katarzyna , Kocbek Simon , Garcia-Cardenas Victoria 
Published: September 2020

Abstract
Background: Implementation research has delved into barriers to implementing change and interventions for the implementation of innovation in practice. There remains a gap, however, that fails to connect implementation barriers to the most effective strategies and provide more tailored interventions during implementation. This study aimed to link implementation barriers to facilitation strategies during a study in community pharmacy and use a data-driven approach to predict the level of effectiveness of facilitation strategies to overcome these barriers.Methods: Six Change facilitators facilitated a two-year change program aimed at implementing professional services across 19 community pharmacies across Australia. A mixed method approach was used where barriers were identified and coded according to implementation factors from the Consolidated Framework of Implementation Research, the Theoretical Domains Framework and the Integrated Checklist of Determinants of practice. Change facilitators trialled and recorded different facilitation strategies to overcome these barriers, until the barrier was resolved. To predict the effectiveness of these strategies a data mining approach named Random Forest was used to provide the highest level of accuracy. Results: At the end of the program, 1,131 data points were recorded by change facilitators. Upon analysis, 36 barriers were identified. The most frequently identified barrier was a ‘lack of ability to plan for change’ (n=184). A list of 111 change facilitation strategies were extracted from the data. These were coded into 16 facilitation categories according to the Taxonomy of Facilitation Strategies. The most effective strategy category to overcome an ‘inability to plan for change’ was to ‘engage stakeholders by creating ownership’ which had a Predictive Resolution Percentage of 84%. Conclusions: Results from this study have provided a better understanding of implementation barriers in community pharmacy and a data-driven approach to predict the effectiveness of facilitation strategies to overcome these barriers. Tailored facilitation strategies may increase the rate of implementation of innovations in healthcare, leading to an industry that can confidently adapt to continuous change.
Clinical Spectrum of COVID-19 in a Pediatric Mexican Population
Authors: Bustos-Cordova Edgar , Castillo-García Daniela , Cerón-Rodriguez Magdalena , Soler-Quiñones Nadia 
Published: September 2020

Abstract
Objective: From the beginning of the COVID-19 pandemic, it has become evident that the spectrum of manifestations in children is different from those seen in adults. In this study, we aimed to describe a broader clinical spectrum of COVID-19 in children.Methods: In this descriptive, prospective study, we enrolled confirmed pediatric patients with COVID-19 who presented to a pediatric tertiary care center from April to July 2020. All patients were confirmed by the SARSCoV-2 RT-PCR test, and we analyzed 24 symptoms and 25 signs.Results: We analyzed 50 patients with COVID-19. From the evaluated signs and symptoms, the most common symptoms were fever, irritability and dry cough, digestive symptoms were frequently found (24%), and the most common signs were pharyngeal hyperemia and irritability. Conclusion: Clinicians should recognize that the clinical spectrum of COVID-19 in children is wider than previously described. Although the classic signs and symptoms are frequent, digestive symptoms should raise suspicion.
Socio-Economic Factors that Influence Household Food Security in West Pokot County, Kenya
Authors: Gatobu Caroline Kathure , Omboto Peter , Mining Pacificah 
Published: September 2020

Abstract
Socio-economic activities of smallholder farmers have been shown to have either negative or positive contribution towards achieving food security. The aim of this study was to investigate the influence of socio-economic factors on household food security in West pokot County, Kenya. The study was carried out using pluralistic approaches where pragmatism drawing from both qualitative and quantitative research approaches was used. Multi stage and systematic random sampling techniques were used to determine a sample size of respondents in respective locations. This study adopted an explanatory research design and employed mixed methods approach. The researcher also drew philosophical inspiration from pragmatic worldview that is problem centred and more focused on real world issues. The target population comprised of 78,946 smallholder households in West Pokot County with a sample size of 297 respondents. Questionnaires, interview schedules and observations were used for collecting data. The collected data was analysed using both and inferential statistics. Qualitative content was achieved through content analysis. The study found out that socio-economic factors including household income, education level, land ownership and household decision making had significant positive influence on the household food security with r2= 0.349. The study therefore concluded that socio-economic factors influenced positively household food security. The study recommended that approaches that would ensure smallholder farmers are able to access credit and financial support so as to invest in farming needs to be availed.
Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19
Authors: Takahashi Yoichiro , Hayakawa Akira , Sano Rie , Fukuda Haruki , Harada Megumi , Kubo Rieko , Okawa Takafumi , Kominato Yoshihiko 
Published: September 2020

Abstract
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide as a pandemic throughout 2020. Since the virus uses angiotensin-converting enzyme 2 (ACE2) as a receptor for cellular entry, increment of ACE2 would lead to an increased risk of SARS-CoV-2 infection. At the same time, an association of the ABO blood group system with COVID-19 has also been highlighted: there is increasing evidence to suggest that non-O individuals are at higher risk of severe COVID-19 than O individuals. These findings imply that simultaneous suppression of ACE2 and ABO would be a promising approach for prevention or treatment of COVID-19. Notably, we have previously clarified that histone deacetylase inhibitors (HDACIs) are able to suppress ABO expression in vitro. Against this background, we further evaluated the effect of HDACIs on cultured epithelial cell lines, and found that HDACIs suppress both ACE2 and ABO expression simultaneously. Furthermore, the amount of ACE2 protein was shown to be decreased by one of the clinically-used HDACIs, panobinostat, which has been reported to reduce B-antigens on cell surfaces. On the basis of these findings, we conclude that panobinostat could have the potential to serve as a preventive drug against COVID-19.