bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk Factors for Polyvascular Involvement in Patients With Peripheral Artery Disease: A Mendelian Randomization Study
Authors: Dikilitas Ozan , Satterfield Benjamin A. , Kullo Iftikhar J. 
Published: December 2020

Background
Atherosclerosis in >1 vascular bed (ie, polyvascular disease), often a feature of peripheral artery disease (PAD), is associated with high morbidity and mortality. We sought to identify risk factors for polyvascular involvement in patients with PAD.

Methods and Results
We performed 2‐sample Mendelian randomization using an inverse‐variance‐weighted approach, to assess 60 exposures including size and lipid content of atherogenic lipoproteins, blood pressure, glycated hemoglobin, and smoking as causal mediators for polyvascular disease in patients with PAD. Genetic instruments for these exposures were obtained from prior genome‐wide association studies. Patients with PAD were from the Mayo Vascular Disease Biorepository, and polyvascular disease (ie, concomitant coronary heart disease, cerebrovascular disease, and/or abdominal aortic aneurysm) was ascertained by validated phenotyping algorithms. Of 3279 patients with PAD, 61% had polyvascular disease. Genetically predicted levels of the lipid content and/or particle measures of very small and small size very low‐density lipoprotein, intermediate‐density lipoprotein, and large low‐density lipoprotein were associated with polyvascular disease: odds ratios (OR) of 1.80 (95% CI, 1.23–2.61), 1.70 (95% CI, 1.17–2.61), and 1.40 (95% CI, 1.09–1.80) per 1 SD increase in genetically determined levels, respectively. Both genetically predicted diastolic and systolic blood pressure were associated with polyvascular disease; OR per 10 mm Hg genetic increase in diastolic and systolic blood pressure were 1.66 (95% CI, 1.19–2.33) and 1.31 (95% CI, 1.07–1.60), respectively.

Conclusions
Lifetime exposure to increased lipid content and levels of very small and small very low‐density lipoprotein, intermediate‐density lipoprotein, and large low‐density lipoprotein particles as well as elevated blood pressure are associated with polyvascular involvement in patients with PAD. Reduction in levels of such exposures may limit progression of atherosclerosis in patients with PAD.
Prognostic Markers and Long‐Term Outcomes After Aortic Valve Replacement in Patients With Chronic Aortic Regurgitation
Authors: Kim Min‐Seok , Kim Jung Hwan , Joo Hyun‐Chel , Lee Sak , Youn Young‐Nam , Lee Seung Hyun 
Published: December 2020

Background
The objectives of the present study were (1) to evaluate the echocardiographic prognostic factors associated with improved left ventricular (LV) systolic function after aortic valve replacement, and (2) to compare the long‐term outcomes after aortic valve replacement in chronic aortic regurgitation (AR) patients with or without LV dysfunction.

Methods and Results

A total of 280 patients who underwent aortic valve replacement because of chronic aortic regurgitation were studied. Patients with reduced LV systolic function (LV ejection fraction [LVEF] <50%; group reduced LVEF [rEF]; N=80) were compared with those with preserved LV systolic function (LVEF ≥50%; group preserved LVEF; N=200). Postoperative clinical outcomes, overall survival, and freedom from cardiac death were compared. Postoperative echocardiographic examinations were reviewed, and changes in echocardiographic parameters were analyzed. The parameters related to LVEF improvement or normalization were evaluated, and risk factors affecting long‐term survival were identified. Follow‐up was complete in 100% of patients, with a median follow‐up of 104.8 months. Overall and cardiac mortality‐free survival rates at postoperative 10 years were 80.1% and 92.9% and 87.3% and 97.2% in groups rEF and preserved LVEF, respectively (
P
=0.036 and
P
=0.058, respectively). LVEF tended to decrease in the early postoperative period but improved thereafter in both groups. Preoperative early diastolic transmitral flow velocity/mitral annular tissue velocity ratio was a parameter of postoperative improvement or normalization of LVEF in all patients (area under the curve, 0.719;
P
=0.003) and in group rEF patients (area under the curve, 0.726;
P
=0.011) with a cutoff value of 12.73. Preoperative early diastolic transmitral flow velocity/mitral annular tissue velocity ratio also was the parameter of overall survival in all patients (hazard ratio [HR], 1.08;
P
=0.001) and in group rEF patients (HR, 1.08;
P
=0.005).

Conclusions
Long‐term outcomes and survival after aortic valve replacement were related to preoperative LV function in patients with chronic aortic regurgitation. Preoperative early diastolic transmitral flow velocity/mitral annular tissue velocity ratio was correlated with the postoperative improvement or normalization of LVEF and long‐term survival, especially in group rEF patients.
Lipid Management in Patients Presenting With Acute Coronary Syndromes: A Review
Authors: Claessen Bimmer E. , Guedeney Paul , Gibson C. Michael , Angiolillo Dominick J. , Cao Davide , Lepor Norman , Mehran Roxana 
Published: December 2020

Abstract
Despite many improvements in its prevention and management, acute coronary syndrome (ACS) remains a major cause of morbidity and mortality in the developed world. Lipid management is an important part of secondary prevention after ACS, but many patients currently remain undertreated and do not attain guideline‐recommended levels of low‐density lipoprotein cholesterol reduction. This review details the current state of evidence on lipid management in patients presenting with ACS, provides directions for identification of patients who may benefit from early escalation of lipid‐lowering therapy, and discusses novel lipid‐lowering medication that is currently under investigation in clinical trials. Moreover, a treatment algorithm aimed at attaining guideline‐recommended low‐density lipoprotein cholesterol levels is proposed. Despite important advances in the initial treatment and secondary prevention of ACS, ≈20% of ACS survivors experience a subsequent ischemic cardiovascular event within 24 months, and 5‐year mortality ranges from 19% to 22%. Knowledge of the current state of evidence‐based lipid management after ACS is of paramount importance to improve outcomes after ACS.
Three Mesoscale Eddy Detection and Tracking Methods: Assessment for the South China Sea
Authors: Xing Tao , Yang Yikai 
Published: December 2020

AbstractComplex topography and the Kuroshio eddy-shedding process produce active mesoscale eddy activity in the South China Sea (SCS). Three eddy detection and tracking methods, the Okubo–Weiss (O-W), vector-geometry (V-G), and winding-angle (W-A) algorithms, have been widely applied for eddy identification. This study provides a comprehensive assessment of the O-W, V-G, and W-A methods in the SCS, including their detection, statistical analysis, and tracking capabilities. The mean successful detection rates of the O-W, V-G, and W-A methods are 51.9%, 56.8%, and 61.4%, respectively. The O-W and V-G methods preferentially detect eddies with medium radii (½°–1°), whereas the W-A method tends to detect eddies with larger radii (>1°). The V-G method identifies an excessive number of weak (radius < ⅓°) eddylike structures in the SCS, accounting for 48.2% of the total eddy number. The highest mean excessive detection rate of the V-G method biases the data on eddy number, probability, and propagation direction. With the lowest mean successful tracking rate (STR), the O-W method might not be suitable for tracking long-lived eddies in the SCS. The V-G method performs well with regard to the overtracking issue and has the lowest mean questionable tracking rate of 1.1%. Among the three methods, the W-A method tracks eddies most accurately, with the highest mean STR of 80.6%. Overall, the W-A method produces reasonable statistical eddy characteristics and eddy tracking results. Each method has advantages and disadvantages, and researchers should choose wisely according to their needs.
Atmospheric River Sectors: Definition and Characteristics Observed Using Dropsondes from 2014–20 CalWater and AR Recon
Authors: Cobb A. , Michaelis A. , Iacobellis S. , Ralph F. M. , Delle Monache L. 
Published: December 2020

AbstractAtmospheric rivers (ARs) are responsible for intense winter rainfall events impacting the U.S. West Coast, and have been studied extensively during CalWater and AR Recon field programs (2014–20). A unique set of 858 dropsondes deployed in lines transecting 33 ARs are analyzed, and integrated vapor transport (IVT) is used to define five regions: core, cold and warm sectors, and non-AR cold and warm sides. The core is defined as having at least 80% of the maximum IVT in the transect. Remaining dropsondes with IVT > 250 kg m−1 s−1 are assigned to cold or warm sectors, and those outside of this threshold form non-AR sides. The mean widths of the three AR sectors are approximately 280 km. However, the core contains roughly 50% of all the water vapor transport (i.e., the total IVT), while the others each contain roughly 25%. A low-level jet occurs most often in the core and warm sector with mean maximum wind speeds of 28.3 and 21.7 m s−1, comparable to previous studies, although with heights approximately 300 m lower than previously reported. The core exhibits characteristics most favorable for adiabatic lifting to saturation by the California coastal range. On average, stability in the core is moist neutral, with considerable variability around the mean. A relaxed squared moist Brunt–Väisälä frequency threshold shows ~8%–12% of core profiles exhibiting near-moist neutrality. The vertical distribution of IVT, which modulates orographic precipitation, varied across AR sectors, with 75% of IVT residing below 3115 m in the core.
App-based learning for kindergarten children at home (Learning4Kids): study protocol for cohort 1 and the kindergarten assessments
Authors: Niklas Frank , Annac Efsun , Wirth Astrid 
Published: December 2020

AbstractBackgroundChildren’s literacy and mathematical competencies are a critical platform for their successful functioning as individuals in society. However, many children, in particular those with low socio-economic status (SES) backgrounds who may not receive the home support needed to develop to their full potential, are at risk of not reaching sufficient competence levels. The overall aim of this project is to develop innovative computer tablet applications (‘apps’) and test whether the apps support parents in the provision of high-quality home learning environments (HLEs) and impact positively on the short- and long-term development of children’s competencies.Altogether, “App-based learning for kindergarten children at home” (Learning4Kids) is a 5-year longitudinal study funded by the EU and designed to assess the potential impact of a tablet-based family intervention on children’s learning, development, social inclusion and well-being.Methods/designThis study uses a multi-method intervention approach and draws on expertise from psychology, education, informatics, and didactics to evaluate the effectiveness of learning apps and the intervention approach. It also exploits new technological possibilities afforded by tablet computers that are very common nowadays in families. Learning4Kids sets out to measure the quality of the HLE, children’s early mathematical, literacy, and cognitive competencies and their behaviour. Here, data will be gathered via standardized tests, observations, and parental and educator surveys and checklists. Data collection also includes the assessment of app usage times via mobile sensing. In cohort 1, 190 families are assigned to one of four groups. One business-as-usual group will only participate in the child assessments, whereas the three remaining groups are provided with tablets for about 10 months. Two intervention groups will receive mathematical or literacy learning apps as well as parental information about these topics and the tablet-control-group will receive similar apps and information that focus on general child development, but not on mathematics or literacy.DiscussionWhilst offering substantive advances for the scientific fields of psychology and education, the Learning4Kids study also has broad societal implications. Improving young children’s learning trajectories is both a social and economic imperative as it equips them to achieve greater individual success and to contribute to societal prosperity.
Intrauterine cytomegalovirus infection: a possible risk for cerebral palsy and related to its clinical features, neuroimaging findings: a retrospective study
Authors: Xu H , Zhang L , Xuan XY , Zhu M , Tang J , Zhao XK 
Published: December 2020

Abstract
Background
Abundant clinical evidences indicate that the increased risk of cerebral palsy (CP) may be associated with the intrauterine exposure to maternal infection. Cytomegalovirus (CMV) is a common cause of CP. However, little is known about the relationship between the intrauterine exposure of the fetus to CMV infection and CP. This study aims to explore the relationships between intrauterine CMV infection and clinical symptoms, classification, intelligence development and brain neuroimaging findings in children with CP.

Methods
In this study, 147 children with CP in recent 6 years were retrospectively analyzed (average age: 14.76 ± 3.07months; sex (M/F): 103/44). 148 children had CMV IgG and IgM positive sera identified by TORCH examination were selected as the control group (average age: 15.10 ± 3.21months; sex (M/F): 102/46), which also undergo the examination of CMV-DNA in urine. The age and sex of children in the control group were matched with those in the CP group. CMV-DNA in urine was detected by CMV fluorescence quantitative PCR, and t-test was performed to analyze the number of copies. For the CP group, standardized rehabilitation treatment was performed and the function of gross motor was evaluated by GMFM scale before and after treatment. The Gesell developmental scale (GDS) was used to assess the level of intellectual development. The classification of CP was conducted and the results of magnetic resonance imaging were analyzed. Finally, the correlations between the copy number of CMV-DNA and the clinical characteristics of children with CP were evaluated by the method of Pearson and Spearman correlation analysis.

Results
The level of CMV infection was negatively correlated with the developmental quotient (DQ) of children with CP. Negative association was found between the level of CMV infection and the level of the gross motor development. The level of CMV infection was positively related with the occurrence probability of spastic quadriplegia. However, no associations were found between the abnormalities of brain tissue and the number of CMV copies. Moreover, CMV infection might add the difficulty of the rehabilitation treatment.

Conclusions
CMV infection is a risk factor for the occurrence of CP in children. Pregnancy examination should be strengthened. Early detection and control of CMV infection may contribute to the rehabilitation of children with CP and reduce the disability and social burden.
Three-dimensional classification of the Lenke 1 adolescent idiopathic scoliosis using coronal and lateral spinal radiographs
Authors: Pasha Saba , Ho-Fung Victor , Eker Malcolm , Nossov Sarah , Francavilla Michael 
Published: December 2020

AbstractBackgroundClassification of the spinal deformity in adolescent idiopathic scoliosis (AIS) remains two-dimensional (2D) as the spinal radiographs remain the mainstay in clinical evaluation of the disease. 3D classification systems are proposed, however are time consuming. We here aim to evaluate the clinical application of a 3D classification system by the use of only posterior-anterior and lateral radiographs in Lenke 1 adolescent idiopathic scoliosis (AIS).MethodsForty Lenke 1 AIS were classified by five observers following a three-step flowchart, developed based on our previous 3D classification system. This 3D classification characterizes the curve in the frontal and sagittal views and infers the third dimension with rules based on prior data to determine the 3D subtypes of the curve. Repeated rating was performed for 20 randomly selected patients in the same cohort. In addition to the classification by the raters, the 3D model of the spines were generated to determine the actual curve subtype based on the algorithm that was originally used to develop the 3D classification system. The interobserver and intraobserver reliability and the classification accuracy were determined for both 3D and axial classifications of the cohort.ResultsThe interobserver reliability was moderate to strong with a kappa value between 0.61–0.89 for 3D and axial classifications. Comparing the mathematical classification and the raters’ classification, the classification accuracy among all raters ranged between 56 and 89%.ConclusionWe evaluated the reliability of a previously developed 3D classification system for Lenke 1 AIS patients when only two-view spinal radiographs are available. Radiologists and orthopedic surgeons were able to identify the 3D subtypes of Lenke 1 AIS from the patients’ radiographs with moderate to strong reliability. The new 3D classification has the potential to identify the subtypes of the Lenke 1 AIS without a need for quantitative 3D image post-processing.
The use of induced pluripotent stem cells in domestic animals: a narrative review
Authors: Scarfone Rachel A. , Pena Samantha M. , Russell Keith A. , Betts Dean H. , Koch Thomas G. 
Published: December 2020

AbstractInduced pluripotent stem cells (iPSCs) are undifferentiated stem cells characterized by the ability to differentiate into any cell type in the body. iPSCs are a relatively new and rapidly developing technology in many fields of biology, including developmental anatomy and physiology, pathology, and toxicology. These cells have great potential in research as they are self-renewing and pluripotent with minimal ethical concerns. Protocols for their production have been developed for many domestic animal species, which have since been used to further our knowledge in the progression and treatment of diseases. This research is valuable both for veterinary medicine as well as for the prospect of translation to human medicine. Safety, cost, and feasibility are potential barriers for this technology that must be considered before widespread clinical adoption. This review will analyze the literature pertaining to iPSCs derived from various domestic species with a focus on iPSC production and characterization, applications for tissue and disease research, and applications for disease treatment.
“Becoming an independent feeder”: infant’s transition in solid food introduction through baby-led weaning
Authors: Utami Ayu Fitria , Wanda Dessie , Hayati Happy , Fowler Cathrine 
Published: December 2020

Abstract
Background
Baby-led weaning (BLW), a method for introducing complementary foods, has become popular because it is considered beneficial for infants.

Methods
This study investigated the experiences of mothers when using BLW in Jakarta, Indonesia using a qualitative descriptive approach. Thirteen mothers participated who had introduced complementary feeding using BLW for a minimum of 6 months. Semi-structured interviews and thematic analysis was used to work with the data.

Results
Three themes were identified: avoiding being a ‘picky’ eater; infants gagging and choking; and becoming independent feeders.

Conclusion
Further research related to the growth and development of baby-led weaning infants in Indonesia is recommended.
Correlations between drug use, HIV disclosure and interpersonal communication on sexual risk behaviour of HIV-positive men who have sex with men
Authors: Afriyanti Lussy , Waluyo Agung , Yona Sri 
Published: December 2020

Abstract
Background
The Human Immunodeficiency Virus (HIV) epidemic is a global health problem whose number of cases are always higher among men who have sex with men (MSM). Most existing MSM have moderate and very high risk behaviour in HIV transmission. This study was designed to identify correlations between drug use, HIV disclosure and interpersonal communication patterns on sexual risk behaviour among HIV-positive MSM.

Methods
This study used a cross sectional design with a purposive sampling technique for participants who visited the voluntary counselling and testing (VCT) clinic in a referral hospital and snowball sampling technique for participants in the work area of a community health centre in Batam involving 126 HIV-positive MSM. Data were collected by 5 part questionnaire, namely demographic questionnaire, drug screening questionnaire, brief scale for HIV self disclosure, communication pattern questionnaire-short form, and safe sex behaviour questionnaire. Bivariate analysis was applied to determine whether there is a relationship between drug use, HIV disclosure, interpersonal communication and demographic characteristics (ethnicity, educational status) with sexual risk behaviour of MSM. Logistic regression analysis was used to explore the variables that most associated variable to sexual risk behaviour.

The results
The results showed that there was significant correlation between HIV disclosure and sexual risk behaviour (p = 0.019, α = 0.05, OR = 2.530) and significant correlation between interpersonal communication patterns and sexual risk behaviour (p = 0.016, α = 0.05, OR = 2.589). There is no significant correlation between demographic characteristics, namely: ethnicity and educational status with sexual behaviour at risk of MSM. In multiple logistic regression analysis, educational status was the factor that most associated with sexual risk behaviour among HIV-positive MSM (p = 0.027, α = 0.05, OR = 2.807, 95% CI = 1.125–7.006).

Conclusions
HIV disclosure and interpersonal communication patterns have a significant negative correlation with sexual risk behaviour among HIV-positive MSM. MSM with low HIV disclosure have high risk sexual behaviour opportunities. MSM with the closed interpersonal communication pattern also has a high risk of sexual behaviour. Education status was the most associated variable to sexual risk behaviour of HIV-positive MSM. Nurses as professional health workers need to improve comprehensive assessment, personal counselling and plan specific learning model by involving HIV-positive MSM in reducing HIV transmission from risky behaviour.
STARRPeaker: uniform processing and accurate identification of STARR-seq active regions
Authors: Lee Donghoon , Shi Manman , Moran Jennifer , Wall Martha , Zhang Jing , Liu Jason , Fitzgerald Dominic , Kyono Yasuhiro , Ma Lijia , White Kevin P. , Gerstein Mark 
Published: December 2020

AbstractSTARR-seq technology has employed progressively more complex genomic libraries and increased sequencing depths. An issue with the increased complexity and depth is that the coverage in STARR-seq experiments is non-uniform, overdispersed, and often confounded by sequencing biases, such as GC content. Furthermore, STARR-seq readout is confounded by RNA secondary structure and thermodynamic stability. To address these potential confounders, we developed a negative binomial regression framework for uniformly processing STARR-seq data, called STARRPeaker. Moreover, to aid our effort, we generated whole-genome STARR-seq data from the HepG2 and K562 human cell lines and applied STARRPeaker to comprehensively and unbiasedly call enhancers in them.