bluuebunny/crossref_metadata_2025_split huggingface dataset:
A unified view of neighbour cell engagement during apoptotic cell extrusion
Authors: Duszyc Kinga , Gomez Guillermo A. , Lagendijk Anne K. , Yau Mei-Kwan , Gliddon Briony L. , Hall Thomas E. , Verma Suzie , Hogan Benjamin M. , Pitson Stuart M. , Fairlie David P. , Parton Robert G. , Yap Alpha S. 
Published: August 2020

AbstractEpithelia must eliminate apoptotic cells to preserve tissue barriers and prevent inflammation [1]. Several different mechanisms exist for apoptotic clearance, including efferocytosis [2, 3] and apical extrusion [4, 5]. We found that extrusion was the first-line response to apoptosis in cultured monolayers and in zebrafish epidermis. During extrusion, the apoptotic cell elicited active lamellipodial protrusions and assembly of a contractile extrusion ring in its neighbours. Depleting E-cadherin compromised both the contractile ring and extrusion, implying that a cadherin-dependent pathway allows apoptotic cells to engage their neighbours for extrusion. We identify RhoA as the cadherin-dependent signal in the neighbour cells and show that it is activated in response to contractile tension from the apoptotic cell. This mechanical stimulus is conveyed by a Myosin VI-dependent mechanotransduction pathway that is necessary both for extrusion and to preserve the epithelial barrier when apoptosis was stimulated. Earlier studies suggested that release of sphingosine-1-phosphate (S1P) from apoptotic cells might define where RhoA was activated. However, we found that although S1P is necessary for extrusion, its contribution does not require a localized source of S1P in the epithelium. We therefore propose a unified view of how RhoA is stimulated to engage neighbour cells for apoptotic extrusion. Here, tension-sensitive mechanotransduction is the proximate mechanism that activates RhoA specifically in the immediate neighbours of apoptotic cells, but this also must be primed by S1P in the tissue environment. Together, these elements provide a coincidence detection system that confers robustness on the extrusion response.
High feature overlap reveals the importance of anterior and medial temporal lobe structures for learning by means of fast mapping
Authors: Zaiser Ann-Kathrin , Bader Regine , Meyer Patric 
Published: August 2020

AbstractContrary to traditional theories of declarative memory, it has recently been shown that novel, arbitrary associations can be rapidly and directly integrated into cortical memory networks by means of a learning procedure called fast mapping (FM), possibly bypassing time-consuming hippocampal-neocortical consolidation processes. In the typical FM paradigm, a picture of a previously unknown item is presented next to a picture of a previously known item and participants answer a question referring to an unfamiliar label. It is assumed that they thereby incidentally create associations between the unknown item and the label. However, contradictory findings have been reported and factors moderating rapid cortical integration through FM yet need to be identified. In the context of previous behavioral results showing rapid semantic integration through FM especially if the unknown and the known item shared many features, we propose that due to its computational mechanisms during the processing of complex and particularly highly similar objects, the perirhinal cortex might be especially qualified to support the rapid incorporation of these associations into cortical memory networks within the FM paradigm. We therefore expected that a high degree of feature overlap between the unknown and the known item would trigger strong engagement of the perirhinal cortex at encoding, which in turn might enhance rapid cortical integration of the novel picture-label associations. Within an fMRI experiment, we observed stronger activation for subsequent hits than misses during encoding in the perirhinal cortex and an associated anterior temporal network if the items shared many features than if they shared few features, indicating that the perirhinal cortex indeed contributes to the acquisition of novel associations by means of FM if feature overlap is high.
Sex, fitness decline and recombination – Muller’s ratchet vs. Ohta’s ratchet
Authors: Ruan Yongsen , Wang Haiyu , Zhang Lingjie , Wen Haijun , Wu Chung-I 
Published: August 2020

AbstractIt is generally accepted that the absence of recombination reduces the efficacy of natural selection for, or against, mutations. A special case is Muller’s Ratchet (MR) whereby non-recombining genomes experience irreversible fitness decline due to the accumulation of deleterious mutations. MR has been a main hypothesis for sexual reproduction as well as many other biological phenomena. We now ask whether the fitness decline can indeed be stopped if an asexual population turns sexual to become recombining. The possible fitness decline under recombination is referred to as Ohta’s Ratchet (OR). In comparison, MR is more effective in driving fitness reduction than OR, but only in a restricted parameter space of mutation rate, population size and selection. Outside of this space, the two ratchets are equally effective or, alternatively, neither is sufficiently powerful. Furthermore, beneficial mutations can affect the population fitness, which may diverge between the two ratchets, but only in a small parameter space. Since recombination plays a limited role in driving fitness decline, the operation of MR could be far less common in nature than believed. A companion report (see Supplement) surveying the biological phenomena attributed to MR indeed suggests the alternative explanations to be generally more compelling.
HIV infection and COVID-19 death: population-based cohort analysis of UK primary care data and linked national death registrations within the OpenSAFELY platform
Authors: Bhaskaran K , Rentsch CT , MacKenna B , Schultz A , Mehrkar A , Bates C , Eggo RM , Morton CE , Bacon S , Inglesby P , Douglas IJ , Walker AJ , McDonald HI , Cockburn J , Williamson EJ , Evans D , Forbes HJ , Curtis HJ , Hulme W , Parry J , Hester F , Harper S , Evans SJW , Smeeth L , Goldacre B 
Published: August 2020

AbstractBackgroundIt is unclear whether HIV infection is associated with risk of COVID-19 death. We aimed to investigate this in a large-scale population-based study in England.MethodsWorking on behalf of NHS England, we used the OpenSAFELY platform to analyse routinely collected electronic primary care data linked to national death registrations. People with a primary care record for HIV infection were compared to people without HIV. COVID-19 death was defined by ICD-10 codes U07.1 or U07.2 anywhere on the death certificate. Cox regression models were used to estimate the association between HIV infection and COVID-19 death, initially adjusted for age and sex, then adding adjustment for index of multiple deprivation and ethnicity, and finally for a broad range of comorbidities. Interaction terms were added to assess effect modification by age, sex, ethnicity, comorbidities and calendar time.Results17.3 million adults were included, of whom 27,480 (0.16%) had HIV recorded. People living with HIV were more likely to be male, of black ethnicity, and from a more deprived geographical area than the general population. There were 14,882 COVID-19 deaths during the study period, with 25 among people with HIV. People living with HIV had nearly three-fold higher risk of COVID-19 death than those without HIV after adjusting for age and sex (HR=2.90, 95% CI 1.96-4.30). The association was attenuated but risk remained substantially raised, after adjustment for deprivation and ethnicity (adjusted HR=2.52, 1.70-3.73) and further adjustment for comorbidities (HR=2.30, 1.55-3.41). There was some evidence that the association was larger among people of black ethnicity (HR = 3.80, 2.15-6.74, compared to 1.64, 0.92-2.90 in non-black individuals, p-interaction=0.045)InterpretationHIV infection was associated with a markedly raised risk of COVID-19 death in a country with high levels of antiretroviral therapy coverage and viral suppression; the association was larger in people of black ethnicity.
Detecting coronavirus contact using internet of things
Authors: M. Thangamani , M. Ganthimathi , S.R. Sridhar , M. Akila , R. Keerthana , P.S. Ramesh 
Published: August 2020

Purpose
The purpose of this paper is to identify coronavirus contact using internet of things. The disease is said to be highly contagious with the contact of infected persons. Feared to be air-borne, droplets of body fluids can transmit the disease in a matter of hours. The predominant symptoms of the COVID-19 are high fever, cough, breathing problem, etc. Recent studies have demonstrated the evolution of the disease to hide its symptoms. As it is highly transmissible, this disease might spread at an exponential rate costing the lives of thousands of people. The chain of transmission has to be detected with utmost priority through early detection and isolation of infected people. Automated internet of things (IoT) devices can be used in design and implementation of a prediction scheme for reporting the health-care risks of the patients with various parameters such as temperature, humidity and blood pressure.

Design/methodology/approach
IoT is a configuration of multiple autonomous and embedded wireless devices for serving a purpose. Every object possesses an individual identity and will serve to register critical events as entries for future learning and decisions. IoT plays an inevitable role in medical industries, detection of vital signs of diseases and monitoring. Among other life-threatening diseases, a new pandemic is on rise among world nations. COVID-19, a novel severe acute respiratory syndrome virus originated from animals in December 2019 and is becoming a serious menace to Governments, despite serious measures of lockdowns.

Findings
In this paper, the authors defined an architecture of an IoT system to predict the Covid-19 disease by getting the data from the human through sensors and send the data to the doctor using mobile, computer, etc. The main goal is early health surveillance by predicting COVID-19. Accordingly, the authors are able to identify both symptomatic and asymptomatic patients, which will help in the early prediction of disease.

Originality/value
Using the proposed method, the authors can save the time of both patient and doctor by ensuring timely medical treatment and contribute toward breaking the transmission chain. In so doing, the method also contributes toward avoiding unnecessary expenses and saving human lives.
The dynamics of IT-business strategic alignment: evidence from healthcare information systems implementation
Authors: Sha Xiqing , Chen Jing (Elaine) , Teoh Say Yen 
Published: August 2020

PurposeThis study intends to reveal how to manage the dynamic process of information technology business (IT-business) strategic alignment; managing this alignment is an unknown yet critical issue that must be addressed by any firm trying to unleash the business value of their IT investments.Design/methodology/approachThis study presents our case study of a healthcare organization after healthcare information systems (HIS) implementation and investigates the strategic alignment between the implemented HIS and the organizational strategy from a dynamic perspective.FindingsTwo different patterns of alignment (i.e. an IT-strategy–driven pattern and a business-strategy–driven pattern) are identified, and a process model of the IT-business strategic alignment is developed. Moreover, this study focuses on the social dimension of strategic alignment and examines the role of this dimension, which is critical and fundamental with respect to other dimensions, in achieving strategic alignment.Research limitations/implicationsThis paper makes important theoretical contributions to the understanding of strategic alignment by taking a dynamic view of alignment, identifying different patterns of alignment, emphasizing the role of social alignment and developing a comprehensive process model.Practical implicationsFrom a managerial perspective, managers should periodically scrutinize the IT-business alignment patterns of their organizations and develop dynamic capabilities for strategic alignment.Originality/valueWhile most of the literature on the dynamics of strategic alignment have focused on confirming the dynamics of strategic alignment and identifying the factors that create dynamics in alignment, this study examines IT-business alignment as a continuous process over time, thus providing a novel perspective. Moreover, while the role of social alignment and its impact on downstream performance remain unclear in the current literature, this study incorporates the social dimension of alignment to investigate the role of this dimension in achieving IT-business strategic alignment.
Multifaceted functional diversity for multifaceted crop yield: Towards ecological assembly rules for varietal mixtures
Authors: Montazeaud Germain , Violle Cyrille , Roumet Pierre , Rocher Aline , Ecarnot Martin , Compan Frédéric , Maillet Guillaume , Fort Florian , Fréville Hélène 
Published: August 2020

Abstract

Ecological theories suggest that higher plant genetic diversity can increase productivity in natural ecosystems. So far, varietal mixtures, that is, the cultivation of different genotypes within a field, have shown contrasting results, notably for grain yield where both positive and negative mixing effects have been reported. Such discrepancy between ecological theories and agronomical applications calls for a better understanding of plant–plant interactions in crops.

Using durum wheat Triticum turgidum ssp. durum as a model species, we investigated the effect of functional trait composition on productivity and grain quality of varietal mixtures by growing 179 highly diverse genotypes in pure stands and 197 two‐way mixtures in field conditions. We quantified the agronomic performance of the mixtures relative to their components grown in pure stands on two variables related to productivity, vegetative biomass yield and grain yield, and one variable related to grain quality, grain protein content. We then analysed the relationship between the relative performance of the mixtures and their functional composition that we characterized with trait means and trait differences on 19 above‐ and below‐ground traits.

We found that biomass and grain yield increased by 4% overall in mixtures relative to single varieties, but that mixing effects were non‐significant for grain protein content. The combined effects of trait means and trait differences explained 12%, 17% and 22% of the variability of relative grain yield, biomass yield and grain protein content, respectively, with different traits affecting productivity and grain quality. Clustering varieties into functional groups allowed us to identify the most beneficial associations for multifaceted agronomic performance.

Synthesis and applications. Functional traits explained a significant part of the relative agronomic performance of mixtures compared to monocultures (12%–22%, depending on the yield component). They can thus serve as a basis to identify groups of varieties whose combinations are expected to generate positive mixing effects, especially for productivity, and without compromising grain quality. Selection could then target convergence between groups for some traits and divergence between groups for other traits using empirically derived relationships between functional traits and agronomic performance as a guideline.
Harnessing microbial volatiles to replace pesticides and fertilizers
Authors: Thomas Gareth , Withall David , Birkett Michael 
Published: August 2020

SummaryGlobal agricultural systems are under increasing pressure to deliver sufficient, healthy food for a growing population. Seasonal inputs, including synthetic pesticides and fertilizers, are applied to crops to reduce losses by pathogens, and enhance crop biomass, although their production and application can also incur several economic and environmental penalties. New solutions are therefore urgently required to enhance crop yield whilst reducing dependence on these seasonal inputs. Volatile Organic Compounds (VOCs) produced by soil microorganisms may provide alternative, sustainable solutions, due to their ability to inhibit plant pathogens, induce plant resistance against pathogens and enhance plant growth promotion. This review will highlight recent advances in our understanding of the biological activities of microbial VOCs (mVOCs), providing perspectives on research required to develop them into viable alternatives to current unsustainable seasonal inputs. This can identify potential new avenues for mVOC research and stimulate discussion across the academic community and agri‐business sector.
Clinical, radiological and pathological outcomes following treatment of primary giant cell tumour of bone with Denosumab
Authors: Murphy Benjamin , Vodanovich Domagoj , Spelman Tim , Gullifer James , Slavin John , Powell Gerard , Pang Grant , Choong Peter 
Published: August 2020

AbstractBackgroundGiant cell tumour of bone (GCTOB) is a relatively uncommon, benign, but locally aggressive neoplasm. Denosumab is a fully human monoclonal antibody with inhibitory effects on receptor activator of nuclear factor kappa‐B ligand that has shown early promise as a possible treatment adjuvant for GCTB. However, much is still unknown about its current indications, long‐term effects, the potential risk for rapid relapse and its involvement in sarcomatous transformation.MethodsWe analysed the outcomes of 154 patients with GCTOB. We assessed clinical outcomes via local recurrence free‐survival, metastatic free‐survival and sarcomatous transformation between those treated without Denosumab and those with neo‐adjuvant Denosumab. Our radiological and pathological outcomes were assessed through independent specialist reviews.ResultsFour (19.0%) patients of the neo‐adjuvant group had local recurrence of disease versus 16 (12.0%) patients in the surgery alone group; this results in a 3.62 times increased likelihood of developing local recurrence (P = 0.030). The median time to local recurrence was shorter for the neo‐adjuvant group (421.5 days versus 788.5 days) (P = 0.01). There was no difference between Denosumab and the surgery groups in terms of metastatic disease (P = 0.45). Two patients in our cohort with GCTOB developed sarcomatous transformation, both were treated with Denosumab.ConclusionOur use of Denosumab tended to be for those patients who had surgically difficult tumours to halt the progression and allow easier resections. Of concern we noted a trend towards increasing recurrence rates with the potential risk for rapid relapse. Furthermore, two cases experienced sarcomatous transformation, which is a growing area of concern within the literature.
Myopia prevalence in Denmark – a review of 140 years of myopia research
Authors: Hansen Mathias Hvidtfelt , Hvid‐Hansen Anders , Jacobsen Nina , Kessel Line 
Published: August 2020

AbstractPurposeTo evaluate potential changes in myopia prevalence in Denmark by revising more than 100 years of myopia research.MethodsA systematic literature search was performed in the PubMed, Embase and Cochrane Library databases. Only studies reporting a myopia prevalence in Denmark were included. Myopia was defined using the definition in individual references. We did not restrict inclusion of studies to specific methods of measuring or evaluating refraction. As refraction changes throughout life, information from available studies was divided in relevant age groups. Chi‐squared test was used when analysing the effect of sex and education on myopia prevalence except when the expected values were beneath 5, where Fisher’s exact test was used. To further compare the effect of sex, we calculated the odds ratio of being myopic for females compared to males.ResultsWe identified 29 Danish studies reporting on prevalence of myopia. The studies were performed between year 1882 and 2018. We found no strong evidence of an increase in myopia prevalence in Denmark. Increasing age was associated with an increased myopia prevalence up to the age of 60 years where after the prevalence decreased. Longer education and more intensive educational load were associated with myopia. Fourteen studies compared the prevalence of myopia between males and females and two of these studies found a significant higher prevalence in females.ConclusionWe evaluated nearly 140 years of myopia research in Denmark and did not find a convincing change in prevalence of myopia which is in contrast to the high prevalence of myopia reported in some parts of the world and the expected rise in myopia as predicted by WHO.
Histone demethylase Jumonji domain‐containing 1A inhibits proliferation and progression of gastric cancer by upregulating runt‐related transcription factor 3
Authors: Ning Ke , Shao Yangguang , He Yuxin , Wang Fei , Cui Xi , Liu Furong , Li Danni , Li Feng 
Published: August 2020

AbstractThe histone demethylase Jumonji domain‐containing 1A (JMJD1A) is overexpressed in multiple cancers and promotes cancer progression. However, the role and mechanism of JMJD1A in gastric cancer (GC) remains poorly understood. Here, we found that JMJD1A could suppress GC cell proliferation and xenograft tumor growth. Using RNA sequencing, we identified runt‐related transcription factor 3 (RUNX3) as a novel target gene of JMJD1A. Mechanistically, we identified that JMJD1A upregulated RUNX3 through co–activating Ets‐1 and reducing the H3K9me1/2 levels at the RUNX3 promoter in GC cells. Functionally, JMJD1A inhibits the growth of GC cells in vivo, which is partially dependent on RUNX3. Moreover, JMJD1A expression was decreased in GC and low expression of JMJD1A was correlated with an aggressive phenotype and a poor prognosis in patients with GC. Importantly, JMJD1A expression was positively associated with RUNX3 expression in GC samples. These studies indicated that JMJD1A upregulates RUNX3 expression via co–activation of transcription factor Ets‐1 to inhibit proliferation of GC cells. Our findings provide new insight into the mechanism by which JMJD1A regulates RUNX3 transcription and suggest that JMJD1A and/or RUNX3 may be used as a therapeutic intervention for GC.
Minimally important differences for interpreting the EORTC QLQ‐C30 in patients with advanced colorectal cancer treated with chemotherapy
Authors: Musoro J. Z. , Sodergren S. C. , Coens C. , Pochesci A. , Terada M. , King M. T. , Sprangers M. A. G. , Groenvold M. , Cocks K. , Velikova G. , Flechtner H.‐H. , Bottomley A. ,   the EORTC Quality of Life, Gastro‐intestinal Groups
Published: August 2020

AbstractAimThe European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire Core 30 (EORTC QLQ‐C30) assesses the health‐related quality of life of patients in cancer trials. There are currently no minimally important difference (MID) guidelines for the EORTC QLQ‐C30 for colorectal cancer (CRC). This study aims to estimate MIDs for the EORTC QLQ‐C30 scales in patients with advanced CRC treated with chemotherapy and enrolled in clinical trials.MethodThe data were obtained from three published EORTC trials that treated CRC patients using chemotherapy. Potential anchors were selected from clinical variables based on their correlation with EORTC QLQ‐C30 scales. Anchor‐based MIDs for within‐group change and between‐group change were estimated via the mean change method and linear regression, respectively, and summarized using weighted correlation. Distribution‐based MIDs were also examined.ResultsAnchor‐based MIDs were determined for deterioration in 8 of the 14 EORTC QLQ‐C30 scales and in 9 scales for improvement, and varied by scale, direction of change and anchor. MIDs for improvement (deterioration) ranged from 6 to 18 (−11 to −5) points for within‐group change and 5 to 15 (−10 to −4) for between‐group change. Summarized MIDs (in absolute values) per scale mostly ranged from 5 to 10 points.ConclusionsThese findings have clinical relevance for the interpretation of treatment efficacy and the design of clinical trials by informing sample size requirements.