bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic analysis reveals root metabolic alteration and induction of huanglongbing resistance by sulphonamide antibiotics in huanglongbing‐affected citrus plants
Authors: Yang Chuanyu , Powell Charles A. , Duan Yongping , Ancona Veronica , Huang Junyuan , Zhang Muqing 
Published: January 2020

AbstractHuanglongbing (HLB) is a devastating disease that affects the entire citrus plant, including the root system. Previous studies have shown that sulphonamide antibiotics can suppress titres of the pathogen that causes HLB, ‘Candidatus Liberibacter asiaticus’ (CLas), and affect root morphology in the plant through unknown mechanisms. To better understand the response of CLas‐infected roots to sulphonamide antibiotics, hydroponic cultures of CLas‐infected citrus roots were treated with sulfadimethoxine sodium (SDX), indole butyric acid (IBA), or water for 60 days to evaluate root metabolism and resistance against CLas via transcriptomic analysis. This study indicated that SDX and IBA treatments increased active root surface area in HLB‐affected citrus, which may be due to root hair and lateral root growth. CLas titres in HLB‐affected citrus roots treated with SDX were lower than those of IBA and control treatments. Function categorization indicated that plant hormone signal transduction and plant–pathogen interaction were the most markedly enriched pathways in the HLB‐affected citrus root after SDX treatment. The expression of genes involved in biosynthesis of auxin and ethylene, which are related to root hair and lateral root growth, were up‐regulated by SDX. Moreover, SDX also induced genes related to the metabolism of jasmonates, brassinosteroids, reactive oxygen species, and secondary metabolites, which are beneficial for resistance against HLB. In conclusion, we propose a model for SDX in regulating metabolic pathways in the root and resistance against CLas in HLB‐affected citrus root, which is beneficial for recovering an HLB‐affected citrus root system and combating CLas.
Judicial review of regulatory decisions: Decoding the contents of appeals against agencies in Spain and the United Kingdom
Authors: Mejía Luis E. 
Published: January 2020

AbstractDespite the important role that courts play to supervise the legality of regulatory agencies' actions, only few comparative studies analyze the contents of judicial appeals against regulatory decisions within European countries. This paper builds on the comparative administrative law scholarship and administrative capacities literature to analyze the content of 2,040 rulings against decisions issued by competition and telecommunications regulators in Spain and the United Kingdom. To understand the substance of the appeals, the study classifies cases according to the alleged administrative principles under breach and the regulatory capacities under challenge. Findings show a clear country‐sector variation regarding the information contained in judicial disputes for both dimensions of analysis, which can be explained as a result of existing differences between the institutional settings of courts. These results offer a more in depth understanding of the political role of judicial oversight over regulatory agencies embedded in different institutional arrangements and policy sectors.
Patient involvement in surgical care–Healthcare personnel views and behaviour regarding patient involvement
Authors: Andersson Åsa , Vilhelmsson Mattias , Fomichov Victoria , Lindhoff Larsson Anna , Björnsson Bergthor , Sandström Per , Drott Jenny 
Published: January 2020

BackgroundAll professions in surgical care have a responsibility to include patients in their health care. By Swedish law, all care should be done in dialogue with the patient. The essential part of health care is the meeting between patient and healthcare professional. In the interaction, a decision can be made, and needs can be identified to a safer care. Previous studies on patient participation have focussed on patients' perspectives in surgical care, but there is a paucity of studies about the personnel’s perspective of estimated patient involvement in surgical care.AimThe aim of this study was to identify and describe healthcare personnel’s view and behaviour regarding patient involvement in surgical care.MethodA quantitative study with various professions was conducted. A validated questionnaire was used, remaining questions grouped under following areas: patient involvement, acute phase, hospital time, discharge phase and questions on employment and workplace.ResultsA total of 140 questionnaires were sent out to a surgical clinic in Sweden, and 102 questionnaires were answered. All professionals stated that clear information is an important part of patient involvement in surgical care. Statistically significant differences existed between the professions in the subscale information. Physicians rated their information higher than the Registered Nurses (p = 0.005) and the practical nurses did (p = 0.001). Hindrances to involving patients were lack of time and other priority tasks.ConclusionsProfessionals in surgical care graded information to be the most important thing for patient involvement. Participation in important decisions, including the possibility to express personal views and ask questions, is important factors for patient involvement. Barriers against patient involvement are lack of time and prioritisation of other work activities.
Exercise training attenuates angiotensin II‐induced vasoconstriction in the aorta of normotensive but not hypertensive rats
Authors: Oliveira Priscila Ramos de , Oliveira Priscilla Bianca de , Rossignoli Patrícia de Souza , Spadella Maria Angélica , Chies Agnaldo Bruno 
Published: January 2020

New Findings
What is the central question of this study?
What are the effects of exercise on Ang II‐induced vasoconstriction in aortas of normotensive rats and how do these effects occur in two‐kidney–one‐clip hypertensive animals?

What is the main finding and its importance?
In two‐kidney rats, exercise training improves the Ang II‐induced vasoconstriction by endothelium‐derived NO released through AT2R activation. This effect of exercise training on the Ang II‐induced vasoconstriction is blunted in two‐kidney–one‐clip hypertensive animals, possibly as a consequence of oxidative stress.

AbstractThis study investigated the effects of both acute exercise and training on the Ang II‐induced vasoconstriction in aorta of normotensive (two‐kidney; 2K) and two‐kidney–one‐clip (2K1C) hypertensive rats, focusing on endothelial mechanisms related to nitric oxide (NO) and prostanoids. Aorta rings of 2K and 2K1C male Wistar rats, sedentary and trained, killed at rest and after acute exercise, were challenged with Ang II in either the absence or the presence of PD 123,319, a selective angiotensin receptor subtype 2 (AT2R) antagonist; Nω‐nitro‐l‐arginine methyl ester (l‐NAME), a non‐selective inhibitor of nitric oxide synthase; indomethacin, a non‐selective inhibitor of cyclooxygenase; or Tiron, an analogue of superoxide dismutase. Aortas of sedentary and trained animals studied at rest were also submitted to histomorphometric analysis. Exercise training reduced the Ang II‐induced vasoconstriction in aorta of 2K but not of 2K1C animals. This reduction of Ang II response in aortas of 2K animals was not found after endothelial removal or treatment with PD 123,319 or l‐NAME. These results suggest that exercise training improves the modulation of Ang II‐induced vasoconstriction in aorta of 2K animals, by endothelium‐derived NO released due to the activation of AT2R. No exercise‐induced change of Ang II response occurred in 2K1C animals, except in the presence of Tiron, which was evidence for reduction of such responses only in resting trained 2K1C animals. In 2K1C animals, NO modulation of Ang II‐induced vasoconstriction might be suppressed by local oxidative stress. Moreover, exercise training slightly reduced the media layer thickness in the aortas of the 2K1C, but not 2K animals, which may indicate cardiovascular protection of these animals.
The Effect of Aspect Ratio on Wall Pressure Fluctuations at a Wing-Plate Junction
Authors: Awasthi M. , Rowlands J. , Moreau D. J. , Doolan C. J. 
Published: January 2020

AbstractMeasurements of the wall pressure fluctuations near a wing-plate junction were made for wings with three different aspect ratios (AR) of 0.2, 0.5, and 1.0 at several angles of attack. The chord-based Reynolds number for each wing was 274,000. The results show that the wall pressure fluctuations are a function of wing AR for cases where AR≤ 1.0. For each wing, the pressure fluctuations are highest upstream of the wing leading-edge due to three-dimensional flow separation; wings with AR = 1.0 and 0.5 show comparable levels, while those with AR = 0.2 show lower fluctuation levels over a wide frequency range. Downstream of the leading-edge, the pressure fluctuations decay rapidly on both sides of the wing until the maximum thickness location after which little variation is observed. The pressure fluctuations downstream of the leading-edge on the suction-side were observed to be comparable for AR = 0.2 and 0.5, while those for AR = 1.0 were higher in magnitude. On the pressure-side, the pressure fluctuations near the leading-edge are a weak function of AR; however, those further downstream remain independent of AR. The pressure fluctuations aft of the wing on the suction-side are more coherent for lower ARs and show higher convection velocity, possibly due to an interaction between the tip and the junction flows for lower ARs.
The Multidisciplinary Process Leading to Return From Underperformance and Sustainable Success in the World’s Best Cross-Country Skier
Authors: Solli Guro Strøm , Tønnessen Espen , Sandbakk Øyvind 
Published: January 2020

Purpose: To investigate the factors associated with underperformance and the subsequent changes in training characteristics and supportive actions when returning to the world’s best cross-country skier. Methods: The participant is the most decorated winter Olympian, with 8 Olympic gold medals, 18 World Championship titles, and 114 World Cup victories. Training data were categorized by training form (endurance, strength, and speed); intensity (low, moderate, and high); and mode (running, cycling, and skiing/roller skiing). In addition, test data were retrospectively analyzed, and interviews were performed with the participant and her support team. Results: After the competitive season, the participant had 8 weeks without systematic training and an evaluation process aiming to detect the factors contributing to underperformance. Here physiological, technical, and psychological challenges were detected. As a consequence, the participant included less high-intensity training (1.2 vs 2.1 sessions/wk, P = .011); more moderate-intensity training (0.9 vs 0.4 sessions/wk, P = .016); and more low-intensity training (6.9 vs 5.9 sessions/wk, P = .036) during the general preparation phase but with similar total endurance training load as previous season. In addition, more strength training (1.6 vs 1.1 h/wk, P = .036) and new ski-specific strength exercises were included. Finally, the athlete’s autonomy when planning and adjusting training was increased, nontraining stressors were reduced, more frequent testing was included, systematic mental training was initiated, her nutritional strategy was adjusted, and her asthma treatment was optimized. Conclusions: Overall, the current case study could be used as a framework for the holistic approach to treating an overtraining condition and for generation of new hypothesis in this exiting area.
Role Commitment and Acceptance in a Sport Context
Authors: Eys Mark , Beauchamp Mark R. , Godfrey Michael , Dawson Kim , Loughead Todd M. , Schinke Robert J. 
Published: January 2020

The objectives of this study were to (a) develop a conceptualization of role acceptance, later situated within the broader concept of role commitment, pertinent to the sport environment; (b) develop a measure integrating direct perceptions of role commitment and the bases of this variable; and (c) determine if role commitment could predict athletes’ intentions to return. To accomplish these objectives, multiple methods were used across 4 projects that leveraged the extant literature on acceptance and commitment perceptions from sport and organizational psychology, engaged athletes in focus groups in a think-aloud protocol, and obtained responses on iterative versions of a new role-commitment questionnaire from over 700 athletes from a variety of competitive and developmental levels. Overall, this approach captured the bases of role commitment (affective, normative, and continuance perspectives), as well as direct perceptions of role commitment, and demonstrated an important link to intentions to return to sport.
Activity of a Long-Acting Echinocandin, Rezafungin, and Comparator Antifungal Agents Tested against Contemporary Invasive Fungal Isolates (SENTRY Program, 2016 to 2018)
Authors: Pfaller Michael A. , Carvalhaes Cecilia , Messer Shawn A. , Rhomberg Paul R. , Castanheira Mariana 
Published: January 2020

We evaluated the activity of rezafungin and comparators, using Clinical and Laboratory Standards Institute (CLSI) broth microdilution methods, against a worldwide collection of 2,205 invasive fungal isolates recovered from 2016 to 2018.
Candida
(
n
= 1,904 isolates; 6 species),
Cryptococcus neoformans
(
n
= 73),
Aspergillus fumigatus
(
n
= 183), and
Aspergillus flavus
(
n
= 45) isolates were tested for their susceptibility (S) to rezafungin as well as the comparators caspofungin, anidulafungin, micafungin, and azoles.
A non–plate tectonic model for the Eoarchean Isua supracrustal belt
Authors: Webb A. Alexander G. , Müller Thomas , Zuo Jiawei , Haproff Peter J. , Ramírez-Salazar Anthony 
Published: January 2020

AbstractThe ca. 3.8–3.6-b.y.-old Isua supracrustal belt of SW Greenland is Earth’s only site older than 3.2 Ga that is exclusively interpreted via plate-tectonic theory. The belt is divided into ca. 3.8 Ga and ca. 3.7 Ga halves, and these are interpreted as plate fragments that collided by ca. 3.6 Ga. However, such models are based on idiosyncratic interpretations of field observations and U-Pb zircon data, resulting in intricate, conflicting stratigraphic and structural interpretations. We reanalyzed published geochronological work and associated field constraints previously interpreted to show multiple plate-tectonic events and conducted field-based exploration of metamorphic and structural gradients previously interpreted to show heterogeneities recording plate-tectonic processes. Simpler interpretations are viable, i.e., the belt may have experienced nearly homogeneous metamorphic conditions and strain during a single deformation event prior to intrusion of ca. 3.5 Ga mafic dikes. Curtain and sheath folds occur at multiple scales throughout the belt, with the entire belt potentially representing Earth’s largest a-type fold. Integrating these findings, we present a new model in which two cycles of volcanic burial and resultant melting and tonalite-trondhjemite-granodiorite (TTG) intrusion produced first the ca. 3.8 Ga rocks and then the overlying ca. 3.7 Ga rocks, after which the whole belt was deformed and thinned in a shear zone, producing the multiscale a-type folding patterns. The Eoarchean assembly of the Isua supracrustal belt is therefore most simply explained by vertical stacking of volcanic and intrusive rocks followed by a single shearing event. In combination with well-preserved Paleoarchean terranes, these rocks record the waning downward advection of lithosphere inherent in volcanism-dominated heat-pipe tectonic models for early Earth. These interpretations are consistent with recent findings that early crust-mantle dynamics are remarkably similar across the solar system’s terrestrial bodies.
Effectiveness of an e-health tennis-specific injury prevention programme: randomised controlled trial in adult recreational tennis players
Authors: Pas Haiko Ivo Maria Franciscus Lodewijk , Pluim Babette M , Kilic Ozgur , Verhagen Evert , Gouttebarge Vincent , Holman Rebecca , Moen Maarten H , Kerkhoffs Gino M , Tol Johannes L 
Published: January 2020

BackgroundDespite reported injury rates of up to 3 per 1000 hours exposure, there are no evidence-based prevention programmes in tennis.PurposeTo evaluate the effectiveness of an e-health prevention programme for reducing tennis injury prevalence.Study designTwo-arm, researcher-blinded randomised controlled trial.MethodsAdult tennis players of all playing levels were randomised in an unsupervised programme lasting 12 weeks (TennisReady group or control group). The primary outcome was the overall injury prevalence over a 16-week period, measured at 2 weekly intervals with the Oslo Sports and Trauma Research Centre questionnaire. Estimates for the primary outcome and associated 95% CIs were obtained using generalised estimating equation models. Secondary outcome scores included prevalence of substantial injuries, overall incidence, adherence and time-loss injuries.ResultsA total of 579 (83%) (TennisReady n=286, control n=293) participants were included in the primary analysis. The mean injury prevalence was 37% (95% CI 33% to 42%) in the TennisReady vs 38% (95% CI 34% to 42%) in the control group (adjusted p-value 0.93). The prevalence of substantial injuries was 11% (95% CI 9% to 14%) in the TennisReady vs 12% (95% CI 9% to 15%) in the control group (p value of 0.79). Analysis of the secondary outcome scores showed no difference between groups. The mean prevalence rates between high (8%) and low (92%) adherent groups were 32% (95% CI 23% to 44%) and 37% (95% CI 33% to 42%), respectively (p value 0.36).ConclusionProviding an unsupervised e-health tennis-specific exercise programme did not reduce the injury rates and should not be implemented.Trial registration numberNTR6443.
Prehospital epidemiology and management of injured children in Kigali, Rwanda
Authors: Dworkin Myles , Nsengimana Vizir , Rosenberg Ashley , Scott John , Riviello Robert , Krebs Elizabeth , Umuhoza Christian , Ntaganda Edmond , Uwitonze Jean Marie , Kabagema Ignace , Dushime Theophile , Jayaraman Sudha 
Published: January 2020

IntroductionPaediatric injuries are a major cause of mortality and disability worldwide, yet little information exists regarding its epidemiology or prehospital management in low-income and middle-income countries. We aimed to describe the paediatric injuries seen and managed by the prehospital ambulance service, Service d’Aide Medicale d’Urgence (SAMU), in Kigali, Rwanda over more than 3 years.MethodsA retrospective, descriptive analysis was conducted of all injured children managed by SAMU in the prehospital setting between December 2012 and April 2016.ResultsSAMU responded to a total of 636 injured children, 10% of all patients seen. The incidence of paediatric injury in Kigali, Rwanda was 140 injuries per 100 000 children. 65% were male and the average age 13.5 (±5.3). Most patients were between 15 and 19 years old (56%). The most common causes of injuries were road traffic incidents (RTIs) (447, 72%), falls (70, 11%) and assaults (50, 8%). Most RTIs involved pedestrians (251, 56%), while 15% (65) involved a bicycle. Anatomical injuries included trauma to the head (330, 52%), lower limb (280, 44%) and upper limb (179, 28%). Common interventions included provision of pain medications (445, 70%), intravenous fluids (217, 34%) and stabilisation with cervical collar (190, 30%).ConclusionIn Kigali, RTIs were the most frequent cause of injuries to children requiring prehospital response with most RTIs involving pedestrians. Rwanda has recently instituted several programmes to reduce the impact of paediatric injuries especially with regard to RTIs. These include changes in traffic laws and increased road safety initiatives.
Biological mechanisms and individual variation in fibrinolysis after major trauma
Authors: Coats Timothy J , Morsy Mohamed 
Published: January 2020

ObjectiveTo understand more about the individual variation in the time course of fibrinolysis following major injury and to assess the potential for stratification of trauma patients for tranexamic acid (TXA) therapy.MethodsA historical dataset (from 2004) was used, consisting of samples from 52 injured patients attended by a medical prehospital system. Blood samples were taken at the incident scene, on arrival in the emergency department, 2.5 hours after hospital arrival and 5 hours after hospital arrival. From the study database, we extracted values for tissue-type plasminogen activator (tPA; an activator of fibrinolysis), one of the plasminogen activator inhibitors (PAI-1; as a natural inhibitor of fibrinolysis) and D-dimer (as a marker of the extent of fibrinolysis).ResultsThe changes over time in median tPA and PAI-1 were mirror images, with initial high tPA levels which then rapidly decreased and low initial PAI-1 levels which slowly increased. There were high levels of fibrinolytic activity (D-dimer) throughout. This pattern was present in patients across a broad range of injury severities.ConclusionsAfter major trauma, there seems to be an early ‘antifibrinolytic gap’ with the natural antifibrinolytic system lagging several hours behind the natural profibrinolytics. An early dose of exogenous antifibrinolytic (TXA) might have its effect by filling this gap. The finding that tPA and subsequent clot breakdown (illustrated by D-dimer formation) are raised in a broad range of patients, with little correlation between the initial fibrinolytic response and markers of injury severity, may be the reason that TXA is effective across a broad range of injured patients.
Does cryoneurolysis result in persistent motor deficits? A controlled study using a rat peroneal nerve injury model
Authors: Shah Sameer B , Bremner Shannon , Esparza Mary , Dorn Shanelle , Orozco Elisabeth , Haghshenas Cameron , Ilfeld Brian M , Gabriel Rodney A , Ward Samuel 
Published: January 2020

BackgroundCryoneurolysis of peripheral nerves uses localised intense cold to induce a prolonged block over multiple weeks that has the promise of providing potent analgesia outlasting the duration of postoperative pain following surgery, as well as treat other acute and chronic pain states. However, it remains unclear whether persistent functional motor deficits remain following cryoneurolysis of mixed sensorimotor peripheral nerves, greatly limiting clinical application of this modality. To help inform future research, we used a rat peroneal nerve injury model to evaluate if cryoneurolysis results in persistent deficits in motor function.MethodsMale Lewis rats (n=30) had their common peroneal nerves exposed bilaterally at the proximal lateral margin of the knee and subsequently underwent cryoneurolysis on one limb and sham treatment on the contralateral limb. Outcomes were evaluated on days 3, 14, 30, 90 and 180. The primary end point was motor function, based on ankle dorsiflexion torque. In addition, sensory function was tested based on von Frey’s filament sensitivity to the peroneal sensory distribution. A subset of animals was sacrificed following functional testing at each time point, and general tissue morphology, connective tissue deposition, and axon counts were evaluated.ResultsMotor deficits in treated limbs were observed at 3 and 14 days but had resolved at time points beyond 1 month. Bilateral sensory deficits were also observed at 3 and 14 days, and also resolved within 1 month. Consistent with motor functional deficits, axon counts trended lower in treated nerves compared with contralateral controls at 3 days; however, axon counts were not significantly different at later time points.ConclusionsWhen applied to a mixed sensorimotor nerve, cryoneurolysis did not result in persistent motor deficits.