bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aging-Relevant Human Basal Forebrain Cholinergic Neurons as a Cell Model for Alzheimer’s Disease
Authors: Ma Shuaipeng , Zang Tong , Liu Meng-Lu , Zhang Chun-Li 
Published: May 2020

Abstract
Background : Alzheimer’s disease (AD) is an adult-onset mental disorder with aging as a major risk factor. Early and progressive degeneration of basal forebrain cholinergic neurons (BFCNs) contributes substantially to cognitive impairments of AD. An aging-relevant cell model of BFCNs will critically help understand AD and identify potential therapeutics. Recent studies demonstrate that induced neurons directly reprogrammed from adult skin fibroblasts retain aging-associated features. However, human induced BFCNs (hiBFCNs) have yet to be achieved. Methods : We examined a reprogramming procedure for the generation of aging-relevant hiBFCNs through virus-mediated expression of fate-determining transcription factors. Skin fibroblasts were obtained from healthy human adults and sporadic AD patients. Properties of the induced neurons were examined by immunocytochemistry and electrophysiology. Results : We established a protocol for a highly efficient generation of hiBFCNs from adult human skin fibroblasts. They show electrophysiological properties of mature neurons and express BFCN-specific markers, such as CHAT, p75NTR, ISL1, and VACHT. As a proof-of-concept, our preliminary results further reveal that hiBFCNs from sporadic AD patients’ fibroblasts exhibit time-dependent TAU hyperphosphorylation in the soma and dysfunctional nucleocytoplasmic transport activities. Conclusions : Aging-relevant BFCNs can be directly reprogrammed from human skin fibroblasts of healthy adults and sporadic AD patients. They show promises as an aging-relevant cell model for understanding AD pathology and may be employed for therapeutics identification for AD.
Establishment of Predictive Model for Related Factors of Systemic Inflammatory Response Syndrome (SIRS) After Percutaneous Nephrolithotomy (PCNL)
Authors: Ju Min , Zheng Jin , Pan li Ying , Gao lin Lin 
Published: May 2020

Abstract
Background:The percutaneous nephrolithotomy (PCNL) is a primary method of stone treatment, but the infection is a very common postoperative complication. The systemic inflammatory response syndrome (SIRS) is a stage of the infection process and a very important early clinical manifestation of sepsis, so identifying the risk factors associated with SIRS after PCNL plays an important role in ensuring patients’ safety and preventing sepsis. Methods:Between September 2016 and September 2017,there were total 352 patients who were diagnosed as renal stone and were treated with PCNL, andincluded in this study at last.Patients were divided into two groups according to whether SIRS occurred or not.The univariate analysis was performed on the related risk factors such as patients' age, gender, number of stone, diabetes et al. Then logistic regression was used for multivariate analysis and established a prediction model.Results:There are 352renal stone patients were treated with PCNL, and 106 patients (30.1%) suffered SIRS after operation.It was found that the operative time, preoperative fever and diabetes could be deemed as risk factors, and the Then logistic regression results indicated that diabetes (OR=2.049, 95%CI 1.008～4.166) and operative time (OR=1.011, 95%CI 1.003～1.019) entered the regression equation.Conclusion: Diabetes and operative time are independent risk factors for SIRS after PCNL, so the probability of SIRS after PCNL can be determined in accordance with these two indicators.
The Professional Contribution of Chiropractors to Danish elite football clubs: A qualitative exploration of role and perceived value in an interprofessional service provision context.
Authors: Hostrup Joachim , Koza Anders , Myburgh Corrie 
Published: May 2020

Abstract
Background: Interprofessional team-based care has been widely adopted in elite level athletic health and performance practice. And although chiropractors can claim some penetration as health care service providers in high level sport, their position as valued members of interprofessional health care teams, especially those built around traditional medical organisational structures, is unclear. In Denmark, the mainstream provider status of chiropractors is more favourable than in most parts of the world. Moreover, chiropractors have been identified as service providers in the Danish premiere football league (Super Liga). However, little is currently known about the professional contribution made by chiropractors in this powerful sporting context.As a first step to map the professional practice landscape of Danish sports chiropractors, this investigation sought to explore the perceived role and perceived value of chiropractors as service providers in elite Danish football clubs. Methods: A comparative qualitative case study using semi-structured individual interviews was conducted. Six Danish Superliga clubs were purposively sampled to compare and contrast instances where chiropractors were absent from the health care team, contracted as in-house health care team members or engaged as external service providers. Triangulated responses were solicited from healthcare co-ordinators, chiropractors and athletes within each club’s organisation through short semi-structured individual interviews. The audio-recorded responses were transcribed verbatim and thematically analysed using a framework approach.Results: Inductive and deductive coding revealed 14 unique codes, which were further organised into 4 code families relating to the role of chiropractors, benefits of utilizing chiropractic care and facilitators and barriers to interprofessional practice. From this framework 3 themes were abstracted, these being: “Broadening horizons”, “In-house preferred to take-away” and “Already covered, or even necessary?”. Conclusion: In this practice context, chiropractors fill the role of musculoskeletal health care service providers. Their perceived value stems from additional expert disciplinary knowledge, improved diagnostic triage and increased treatment flexibility. However, where not utilized, the role of the spinal health expert is questioned and when acknowledged, limited to that of a technician/therapist. It is unclear whether chiropractors can claim core team member status. Further exploration of this interesting context of IPP practice is warranted.
Lncrna-Associated Cerna Networks in Spleen of Nocth1-Correlated T-all Leukemia Mice
Authors: Shi Min , Wang Shaohua , Li Xiaofei , Li Yongjun 
Published: May 2020

Abstract
BackgroundAcute T-lymphocytic leukemia (T-ALL) is a highly aggressive malignant tumor in leukemia.Nocth1 is considered as an major oncogene in the development of T-ALL. Increasing evidences have revealed that the occurrence and progression of T-ALL referred to abnormal gene expression, pathway activation and the regulation between these genes. However, the potential lncRNA-associated competing endogenous RNA (ceRNA) network involved in spleen of Nocth1-correlated T-ALL leukemia mice remains unclear.ResultsThe deep RNA-sequencing analysis shown that 1833 lncRNAs and 4626 mRNAs were deregulated according to the P-value (p<0.05) and fold change (>2-fold) in spleen of T-ALL leukemia mice compared with that of C57BL/6 mice. Gene Ontology(GO) and KEGG pathway analysis were performed to reveal the potential roles of differentiated expressed lncRNAs. Co-expression Network was performed to reveal the regulation relationship between the differentiated expressed lncRNAs and mRNAs. CeRNA prediction constructed the lncRNA-miRNA-mRNA model to find the core ceRNA based on regression model analysis and seed sequence matching methods.ConclusionThis study provided a systematic overview of the altered lncRNAs and mRNA expression, pathway and ceRNA regulation network in the pathogenesis of Nocth1-correlated T-ALL.
Angiogenic properties of human endothelial colony-forming cells in Granulomatosis with Polyangiitis
Authors: Rio Ana Paula Toledo Del , Prieto Stephanie Ospina , Frade Jessica Oliveira , Duarte Bruno Lino Kosa , Bertolo Manoel Barros , Ozelo Margareth Castro , Sachetto Zoraida 
Published: May 2020

Abstract
BACKGROUND

Endothelial progenitor cells are essential for vascular homeostasis. Considering the recurrent nature of granulomatosis with polyangiitis (GPA) the aim of the study was to evaluate the angiogenic capacity of endothelial colony-forming cells (ECFC), which have the capacity for neovasculogenesis in vitro, of the patients with GPA, before and after plasma stimulation.
METHODS

Thirteen GPA PR3-positive patients and 15 healthy controls were included. ECFC were isolated from periferic blood and characterized by flow cytometry (FACS). Capillary tube formation (Matrigel assay) and scratching assay were measured during 24 hours. The migration assay was also performed after overnight incubation with healthy control plasma and active GPA patient plasma. Three patients with active disease where submitted to recollection of ECFC after treatment for longitudinal evaluation.
RESULTS

The culture was successful in 62% of GPA patients and 57% of controls. In the matrigel assay the ECFC from patients and controls showed similar capillary structures formation, however ECFC from inactive GPA alone showed early loss of angiogenic capacity between 15 and 24 hours. In scratching assay, there was an impairment in the proliferative capacity of the ECFC between GPA patients and controls without significant difference (12th hour, p = 0.05). When incubated with control plasma, ECFC of remission GPA patients showed a significant lower migration capacity after the 4th hour (p = 0.0001). In longitudinal analysis, ECFC isolated after treatment showed significantly lower migration rates.
CONCLUSIONS

PR3-positive remission GPA ECFC demonstrated early loss of tube formation and less proliferative capacity compared to those of healthy controls, suggesting impairment of endothelial function.
Reliability and validity of Simplified Chinese version of the Italian Spine Youth Quality of Life questionnaire in adolescents with idiopathic scoliosis
Authors: Liu Shanshan , Liang Junyang , Xu Nanfang , Mai Shuo , Wang Qi , Zeng Lin , Du Chaojun , Du Yaoxu , Zeng Yan , Yu Miao , Liu Zhongjun 
Published: May 2020

Abstract
Background: The Italian Spine Youth Quality of Life (ISYQOL) questionnaire is a tool used to evaluate health-related quality of life in adolescents with Idiopathic Scoliosis. The study aimed to undertake the process of cultural adaptation of the ISYQOL questionnaire into Simplified Chinese. Methods: The translation from Italian into Simplified Chinese was performed. It involved 138 adolescents whose Cobb angle ranged between 20-40 degrees, 50 wearing the brace and 88 not wearing the brace. Statistical analysis calculated the reliability, floor and ceiling effects of the ISYQOL. After that, construct validity was measured by analyzing the ISYQOL relationship Scoliosis Research Society-22 patient Questionnaire (SRS-22). Results: There were no floor or ceiling effects in ISYQOL questionnaire. Cronbach’s alpha coefficient evaluated for Internal consistency was 0.75 without the brace and 0.88 with the brace. Intraclass correlation coefficients assessed with the use of the test-retest method was 0.72 without the brace and 0.80 with the brace. A strong relationship exists between the ISYQOL measure and SRS-22 scores (rho=0.63; p< 0.01), reflecting the high validity of the questionnaires. Both ISYQOL and SRS-22 scores showed no statistical difference between groups with and without the brace (p>0.05). Conclusions: Trans-cultural validation in Chinses language showed the reliability and validity of the ISYQOL
Multisystem inflammatory syndrome with features of Atypical Kawasaki disease during COVID-19 pandemic: Report of a case from India
Authors: Rauf Abdul , Vijayan Ajay , John Shaji Thomas , Krishnan Raghuram A , Latheef Abdul 
Published: May 2020

Abstract
There is a global concern of increasing number of children presenting with inflammatory syndrome with clinical features simulating Kawasaki disease, during ongoing COVID-19 pandemic. We report a very similar case of 5-year-old boy from a COVID-19 hotspot area in Kerala state of India who presented in late April 2020 with acute febrile illness with abdominal pain and loose stools followed by shock. On examination, child had bulbar conjunctivitis and extremity edema. Initial investigations showed high inflammatory parameters, elevated serum creatinine and liver enzymes. Echocardiography showed moderate LV dysfunction and normal coronaries. Cardiac enzymes were also elevated, suggesting myocarditis. He was treated with inotropic support, respiratory support with High Flow Nasal Cannula, IV Immunoglobulins, aspirin, steroids and diuretics. RT PCR for SARS-CoV-2 was negative twice. His clinical condition improved rapidly, was afebrile from day 2, inflammatory parameters decreased, left ventricular function improved and was discharged after 6 days of hospital stay.
Evaluation of antidepressant activity of Acorus calamus L. rizhome extract in mice
Authors: Yousuf Shaista , Rasool Akhtar , Haq Shiekh Marifat Ul , Zulfajri Muhammad , Hanafiah Marlia M. , Huang Genin Gary , Mahboob Mohammed 
Published: May 2020

Abstract
Background

Acorus calamus (AC) L. (Araceae) is an annual semi-aquatic and aromatic plant found in Europe, North America and Asia. Its rhizomes are often used by Native Americans, Americans, and Chinese as well as by other cultures. Ethnobotanical studies and documents have shown their use in various disease treatments, such as insomnia, mental disorders, diabetes mellitus, epilepsy, inflammation, asthma, neuropathic pain, and diarrhea. In this study, the antidepressant activity of methanolic and hydroalcoholic extracts of the AC rhizome part in mice was investigated.
Materials and methods

Three doses of methanolic extract of AC rhizome (MEACR) (25, 50 and 100 mg/kg b.wt), three doses of hydroalcoholic extract of AC rhizome (HAACR) (100, 200 and 400 mg/kg b.wt), and standards (imipramine, 15 mg/kg b.wt and fluoxetine, 20 mg/kg b.wt) was daily oral administration to the mice for consecutive 14 days. The extract effect on the immobility time was monitored by a tail suspension test (TST) and a forced swimming test (FST). Monoamine oxidase (MAO) levels were also analyzed using standard methods.
Results

The optimum antidepressant activity was viewed at 100 mg/kg b.wt of MEACR extract and 400 mg/kg b.wt of HAACR extract with 23.82% and 20.59% immobility period reduction, respectively. Besides, the extracts weakened the FST-induced elevation of MAO activity significantly and returned to near-normal levels of neurotransmitters in the brain. 100 mg/kg b.wt or above of MEACR extract significantly prevented the MAO-A and MAO-B activities in mice brain at a dose-dependent fashion. But, just 400 mg/kg b.wt of HAACR extract prevented the activity of MAO-A and MAO-B. Fluoxetine and imipramine showed a tendency to prevent the activity of MAO-A and MAO-B.
Conclusion

This study suggests that AC rhizome extract mediated antidepressant activity by modulating the central neurochemical and hypothalamic-pituitary-adrenal (HPA) axis in response to FST and TST-induced stress. Therefore, AC rhizome extract can be used as a valuable plant supplement to treat depressive disorders.
Myopia-26, the female-limited form of early-onset high myopia is associated with retinal ganglion cell dysfunction and primary open angle glaucoma
Authors: Széll Noémi , Feher Tamas , Maróti Zoltán , Kalmár Tibor , Latinovics Dóra , Nagy István , Orosz Zsuzsanna Z. , Janáky Márta , Facskó Andrea , Sohajda Zoltán 
Published: May 2020

Abstract
Background: Female-limited early-onset high myopia, also called Myopia-26 is a rare monogenic disorder characterized by severe short sightedness starting in early childhood and progressing to blindness potentially by the middle ages. Despite the X-linked locus of the mutated ARR3 gene, the disease paradoxically affects females only, with males being asymptomatic carriers. Previously, this disease has only been observed in Asian families and has not gone through detailed investigation concerning collateral symptoms or pathogenesis Results: We found a large Hungarian family displaying female-limited early-onset high myopia. Whole exome sequencing of two individuals identified a novel nonsense mutation (c.214C>T, p.Arg72*) in the ARR3 gene. We carried out basic ophthalmological testing for 18 family members, as well as detailed ophthalmological examination (intraocular pressure, axial length, fundus appearance, optical coherence tomography, visual field- and colour vision testing) and electrophysiology tests (standard and multifocal electroretinography, pattern electroretinography and visual evoked potentials) for eight individuals. We found variously impaired visual fields in four affected myopic females and a carrier male. Abnormal pattern electroretinography responses were detected in both symptomatic and carrier patients. Conclusions: This is the first description of a Caucasian family displaying Myopia-26. Pattern electroretinography implies retinal ganglion cell dysfunction present in both sexes. In conjunction with the visual field loss, this suggests the development of age-related open angle glaucoma in addition to high myopia, both attributable to the rare genetic variant of ARR3. We present two hypotheses that could potentially explain the pathomechanism of this disease.
A novel multi-epitope recombinant protein for diagnosis of African swine fever
Authors: GAO ZHAN , SHAO JUNJUN , ZHANG GUANGLEI , GE SUDAN , CHANG YANYAN , XIAO LEI , CHANG HUIYUN 
Published: May 2020

Abstract
Background: African swine fever(ASF) is an acute, severe and highly fatal infectious disease of pigs. The disease spreads rapidly, causing huge economic losses to the pig industry in infected areas. The structural proteins p30 and p54 in African swine fever virus(ASFV) have been verified as diagnostic antigens.Methods: In this study, we constructed a novel multi-epitope fusion antigen gene based on P30 and P54 proteins, induced expression in a prokaryotic expression system and analyzed the reactivity of the recombinant fusion protein. The purified recombinant protein m35 was used as the coating antigen to establish an indirect enzyme-linked immunosorbent assay (ELISA) detection method for ASFV. 116 serum samples and positive sera of other swine diseases were detected by indirect ELISA.Results: Our results indicate that the m35 gene fragment with a length of 558bp was successfully constructed. SDS-PAGE and Western Blotting analysis showed that the protein had a band at 22kDa, proving its good reactogenicity. ROC analysis was performed to validate the assay, the area under the ROC curve is 0.9738 (95% confidence interval, 0.9336 to 1.014), and does not cross-react with other swine diseases.Conclusion: Our results show that its sensitivity and specificity were highly accurate. It is feasible to use this recombinant protein as a diagnostic antigen to distinguish ASFV infection.
Test anxiety and emotion regulation among undergraduate medical students in China: the mediating role of psychological resilience
Authors: Liu Yuguo , Pan Haiyan , Yang Runhuang , Wang Xingjie , Rao Jiawei , Zhang Xingshan , Pan Congcong 
Published: May 2020

Abstract
Background: Medical students experience a considerable amount of anxiety due to exams. Emotion regulation and psychological resilience are established protective factors of individual mental health, however, the investigations for the effects of anxiety were limited. The goal of the present study was to examine the relationship of psychological resilience and emotion regulation with test anxiety and the associated factors of them among medical students.Methods: In this cross-sectional survey, a simple random sampling methods was used to select the participants. Information from a sample of 1266 medical students was collected by self-reporting questionnaires. Logistic regression was applied to test the associations between test anxiety and emotion regulation, resilience. Bootstrap were conducted to explore the mediating role of resilience.Results: Our important results were that the prevalence of problematic test anxiety among medical students to be 71.4%, 33.7% was high test anxiety. Gender and academic performance correlated significantly with test anxiety, emotion regulation, and psychological resilience. There were correlations between test anxiety and various dimensions of emotion regulation and psychological resilience ( P<0.01 ). Emotion regulation and psychological resilience predicted emerging test anxiety. The mediating role of psychological resilience was identified for the effects of emotion regulation on test anxiety.Conclusions： Findings suggest that emotion regulation affected test anxiety through psychological resilience, which may provide insights for clinical psychologists, raise their awareness of the importance of cultivating and improving medical students' psychological resilience, and prompt them to offer psychological support to students with test anxiety as early as possible. The combination of curing and self-healing can solve the root cause of the problem and truly apply psychological research to improving the mental health of the general public.
Serum biomarkers of the calcium-deficient rats identified by metabolomics based on UPLC/Q-TOF MS/MS
Authors: Fanyu Meng , Lina Fan , Lin Sun , Qingli Yu , Wang Maoqing , Changhao Sun 
Published: May 2020

Abstract
Background: We previously identified the urinary biomarkers to diagnose calcium deficiency and nutritional rickets by ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF MS/MS).To further confirm these biomarkers in vivo, we performed serum metabolomics analysis of calcium deficiency.Methods: A calcium-deficient rat model was established with a low-calcium diet for 12 weeks. Serum-metabolomics-based UPLC/Q-TOF MS/MS and multivariate statistical analysis was performed to identify the alterations in metabolites associated with calcium deficiency in rats.Results: Bone mineral density, serum parathyroid hormone and alkaline phosphatase were significantly decreased in the low-calcium diet group (LCG) compared to the normal calcium diet group (NCG). Serum metabolic-profiling analysis could definitively distinguish between the LCG and NCG andidentified25 calcium-deficient biomarkers. Three metabolites (indoxyl sulfate, phosphate, and taurine) of the 25 biomarkers were found in our previous urinary metabolomics study of rats with a calcium deficiency and nutritional rickets. The areas under the curve (AUCs) of these three biomarkers were greater than 0.8, and the combination of any two biomarkers was higher than 0.95.Conclusion: Dietary calcium deficiency induced the alterations of metabolites in the serum of rats, and the three identified biomarkers had relatively high diagnostic values for calcium deficiency in rats.hatase were significantly decreased in the low-calcium diet group (LCG) compared to the normal calcium diet group (NCG). Serum metabolic-profiling analysis could definitively distinguish between the LCG and NCG andidentified25 calcium-deficient biomarkers. Three metabolites (indoxyl sulfate, phosphate, and taurine) of the 25 biomarkers were found in our previous urinary metabolomics study of rats with a calcium deficiency and nutritional rickets. The areas under the curve (AUCs) of these three biomarkers were greater than 0.8, and the combination of any two biomarkers was higher than 0.95. Conclusion Dietary calcium deficiency induced the alterations of metabolites in the serum of rats, and the three identified biomarkers had relatively high diagnostic values for calcium deficiency in rats.