bluuebunny/crossref_metadata_2025_split huggingface dataset:
MÚSICA COMO ESTRATÉGIA PARA O CUIDADO NO ENFRENTAMENTO DA COVID-19: UM RELATO DE EXPERIÊNCIA
Authors: Souza Jeane Barros de , Brum Crhis Netto de , Tomasi Yaná Tamara , Massaroli Aline , Silva Filho Cláudio Claudino da 
Published: September 2020

<p><strong>Objetivo:</strong> compartilhar a vivência do desenvolvimento de um vídeo musical por docentes de um curso de graduação em Enfermagem para os discentes, como forma de demonstrar cuidado e apoio no enfrentamento da COVID-19. <strong>Método:</strong>trata-se de um relato de experiência da construção de um vídeo musical, envolvendo 32 enfermeiros, docentes do curso de graduação em enfermagem de uma Universidade pública do Sul do Brasil. <strong>Resultados: </strong>o vídeo musical foi estruturado para que pudesse refletir a relevância dos discentes no cotidiano da Universidade. A música balizou os sentimentos que são necessários externalizar nesse período de pandemia, que ocasionalmente, perpassaram as mudanças abruptas relativas ao vivido. <strong>Conclusão:</strong> a estratégia mostrou-se promotora de saúde e articuladora entre os docentes e discentes, apontando novas perspectivas de cuidado que ultrapassam as barreiras da presencialidade, vislumbrando o uso das tecnologias em prol da ampliação dos horizontes para o cuidado em tempos de distanciamento social e quarentena.</p>
Financial Case Analysis of Chang'an Bank Rural Revitalization Loan
Authors: Yang Jiayuan 
Published: September 2020

<p class="MsoNormal"><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 宋体; mso-font-kerning: 1.0000pt;"> On the basis of the rapid development of the new rural financial institutions as HP’s “three rural” financial system, Chang’an Bank also participated in the promotion of support based on industry support. Taking HP finance as the grasp, the city commercial bank is implemented to support rural revitalization when the effectiveness and accuracy of precision poverty alleviation is improved. Combined with the strategy of rural revitalization, this paper analyzes the advantages and constraints of the financial innovation case of “rural revitalization loan” put forward by Chang’an Bank, and it puts forward some suggestions.</span></p><p class="MsoNormal" style="margin-top: 70pt; margin-bottom: 8pt; text-indent: 0pt;"><strong><span style="font-family: 'Times New Roman'; font-size: 16pt;">Financial Case Analysis of Chang’an Bank Rural Revitalization Loan</span></strong><strong></strong></p>
The influence of plastic techniques in surgery of primary skin melanoma on patient survival
Authors: Yargunin S. A. , Shoyhet Y. N. , Lazarev A. F. 
Published: September 2020

Objective. To analyze the feasibility of performing plastic surgery in patients with primary skin melanoma (SM).
Material and methods. We studied patients with primary MK treated in our institution in 2013 (n = 333), who were randomized to a group of 2 blind selection methods to the main one (n = 168), in which the tumor removal operation in patients ended with a tissue defect repair and a comparison group ( n = 165) (after removal of the tumor, simple linear suturing of the wound was performed). A statistically significant difference was found in the comparison groups in the occurrence of negative dynamics (ND), progression-free survival (PFS) and overall survival (OS) in patients with MK 0-IIA st during the follow-up period up to 36 months.
Results. It was found that patients with 0-IIA st who underwent plastic surgery to close the defect when removing primary SM have a statistically proven advantage in ND, PFS, and OS compared with patients without plastic surgery for up to 36 months. In general, the use of plastics has a statistical tendency towards the dynamics of an increase in PFS and OS in the early stages of SM.
Discussion. In the early stages (0-IIA) up to 36 months, cases of negative dynamics (4.2%) were observed 2.3 times less frequently than in the comparison group (9.7%) (p = 0.048), and fatal outcomes in the main group (1.8%) were observed 3.7 times less than in the comparison group (6.7%) (p = 0.028). The analysis also shows that in patients who underwent surgery using plastic surgery statistically significantly reduces the risk of distant metastasis by 3 times (p = 0.05), but significantly more often (in every third patient) (p = 0.022) than in the control group (without plastic surgery) met transient metastases. The appearance of ND, as well as an increase in PFS, OS depended on the plastic replacement of the defect after excision of the primary SM in patients with SM 0-IIA st during the observation period up to 36 months.
Conclusion. The use of plastic methods for closing a wound defect reduces the risk of distant metastasis by 3 times compared with linear suturing, provides a reduction in mortality in patients with SM 0 IIA st for 60 months, prolongs the patients life by an average of 10 months and is the operation of choice in this category.
Modern accents in the diagnostics of inflammatory diseases of the pelvic organs
Authors: Selikhova M. S. , Soltys P. A. 
Published: September 2020

The clinical course of inflammatory diseases of the pelvic organs (PID) is very diverse, certain difficulties in the diagnosis of acute inflammation can be caused with a sufficient combination of probable symptoms. Most of the patients are subacute or chronic course of the disease. Not timely diagnosis and incorrectly prescribed treatment favorably affects the movement of the inflammatory process in the upper genital tract.
The diagnosis of PID in emergency rooms and clinics is often based on clinical criteria, with or without additional laboratory and visual tests. Clinical data have a sensitivity of 87% and a specificity of 50% versus 83% sensitivity and 26% specificity of endometrial culture.
The presence of leukocytosis in peripheral blood, elevated ESR and/or the level of C-reactive protein are also markers of inflammation and may be an indicator of the severity of the disease, but they do not serve as specific signs of PID.
The frequency of occurrence of inflammatory diseases of the reproductive system and common complications can lead to a pronounced imbalance in the reproductive potential of not only women, but also the population. The presence of a predominance of the chronic course of the disease, specialists of different profiles should have a sense of suspicion of the probable presence of an infectious agent in the pelvic organs in women of reproductive age in the presence of various non-specific complaints from the lower abdomen and the absence of other obvious reasons.
Untimely treatment of PID is closely associated with the deterioration of the patients condition and long-term complications.
To achieve successful attempts to prevent complications of PID, it is practically not possible, despite numerous studies.
The widely used routine laboratory examination methods have diagnostic value only in patients with pronounced symptom complexes, while in the chronic course their significance is small.
Bioengineering microfluidic organoids-on-a-chip
Authors: Nikolaev Mikhail , Mitrofanova Olga , Broguiere Nicolas , Geraldo Sara , Tabata Yoji , Brandenberg Nathalie , Gjorevski Nikolche , Lutolf Matthias 
Published: September 2020

Abstract
Organoids derived from epithelial stem cells have emerged as powerful platforms to model development and disease in a dish1-3. However, the current mismatch in anatomy, lifespan and size between native organs and their in vitro counterparts severely limits their applicability4. In particular, the closed, cystic architecture of most epithelial stem cell-derived organoids makes experimental manipulation and assay development cumbersome. Here we describe how tissue engineering and cellular self-organization can be combined to guide in vitro organogenesis into openly accessible, functional intestinal tubes termed ‘mini-guts’. Intestinal stem cells (ISCs) rapidly generate simple columnar epithelia when propagated inside basal lamina-like hydrogel scaffolds that feature a tubular and crypt-containing, in vivo-like anatomical structure. Using a microfluidic perfusion system, dead cells shed into the lumen can be continuously removed from the mini-guts. This increases tissue lifespan to months, establishing a homeostatic organoid culture system in which cell proliferation (in crypts) is balanced with cell death (in villus-like domains). The approach developed here can be extended to generate functional tissue/organ models from other epithelial cell types, including primary human stem/progenitor cells from the small intestine, colon or airway, permitting reconstitution of complex organ-level physiology and disease in a personalized manner.
A study of the mechanism of lncRNA-CR594175 in regulating proliferation and invasion of hepatocellular carcinoma cells in vivo and in vitro
Authors: Liu Quan , Yu Xuxu , Yang Minjie , Li Xiangke , Zhai Xuejia , Lian Yujin , Chen Zhong , Fan Qingxia , Song Lijie , Li Wencai 
Published: September 2020

Abstract
Abstract Background Hepatocellular carcinoma (HCC) is one of the cancers of highest incidence and mortality worldwide. The proliferation and invasion of tumor cells are the main reason for poor prognosis after HCC surgery. Long non-coding RNA (lncRNA) has been shown to play a key role in the progression of HCC. LncRNA-CR594175 is one of the highly expressed lncRNAs in HCC tumors and their metastatic tumors that we have obtained by the High-throughput screening method.Methods To elucidate the role of lncRNA-CR594175 in regulating the proliferation and invasion of human hepatoma cell line, HepG2, we operated through lncRNA-CR594175 silencing to inhibit the progression of HCC, either through in vitro or in vivo experiments.Results We found that lncRNA-CR594175 was lower in adjacent non-cancerous tissues than in primary HCC, and was lower in primary HCC than in its metastasis. Silencing of lncRNA-CR594175 inhibited the proliferation and invasion of HepG2 cells and growth of subcutaneous tumors. The results revealed that lncRNA-CR594175, as a RNA sponge, broke the negative regulation of hsa-miR-142-3p on Catenin, beta-1 (CTNNB1), and once lncRNA-CR594175 was silenced, the hsa-miR142-3p regained its negative regulation on CTNNB1 which can promote HCC progression by activating the wnt pathway. Conclusions Our present study demonstrated for the first time that lncRNA-CR594175 silencing suppressed proliferation and invasion of HCC cells in vivo and in vitro by restoring the negative regulation of hsa-miR-142-3p on CTNNB1, laying a solid theoretical base for using lncRNA-CR594175 as genetic target therapy for HCC and offering a reasonable explanation for inactivation of miRNA in different tumors or in the tumor at different stages.
Changes in Alanine Aminotransferase in Adults with Severe and Complicated Obesity during a Milk-Based Meal Replacement Programme
Authors: Abdalgwad Razk , Rafey Mohammed Faraz , Murphy Conor , Ioana Iulia , O'Shea Paula Marie , Slattery Eoin , Davenport Colin , O'Keeffe Derek Timothy , Finucane Francis Martin 
Published: September 2020

Abstract
Introduction: Excess adiposity is associated with fat accumulation within the liver, and non-alcoholic steatohepatitis (NASH) is highly prevalent in bariatric patients. Elevated alanine aminotransferase (ALT) is associated with prevalent NASH. We sought to determine the influence of a milk-based meal replacement weight-loss programme on ALT levels in adults with severe and complicated obesity.Methods: We conducted a retrospective cohort study of patients who completed a 24-week meal replacement programme, comprised of a weight loss phase followed by weight stabilisation and maintenance phases, each eight weeks long. ALT was quantified using an enzymatic assay with spectrophotometric detection. We examined changes over time in ALT using the non-parametric Wilcoxon singed-rank test and the Friedman test.Results: Of 105 patients, 56 were female, mean age was 51.2±11.2 (range 18.0-71.6) years. There was an unanticipated but transient increase in ALT from 28.0 [20.0, 40.5] iu/l at baseline to 40.0 [26.0, 55.0] iu/l after two weeks (p<0.0005), followed by a gradual reduction to 21.0 [17.0, 28.3] iu/l by 24 weeks (p<0.0005). The overall reductions in ALT were more pronounced in patients who had elevated levels at baseline. Body weight decreased from 144.2±28.0 kg at baseline to 121.6±25.4 kg at 24 weeks (p<0.0005) and body mass index (BMI) decreased from 50.7±8.1 kg m-2 at baseline to 43.0±7.6 kg m-2 by 24 weeks (p<0.0005).Conclusion: In adults with severe and complicated obesity undergoing a milk-based meal replacement programme, there was an initial unanticipated rise in ALT in the first two weeks, followed by a gradual overall reduction by 24 weeks. These findings suggest that rapid weight loss secondary to significant caloric restriction might induce a transient deterioration in hepatic steatosis prior to an ultimate overall improvement.
Genetic diagnostic features after failure of initial treatment with epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors among non-small-cell lung cancer patients harboring EGFR mutations
Authors: Takeda Yuichiro , Naka Go , Yamaguchi Yoh , Hashimoto Masao , Suzuki Manabu , Izumi Shinyu , Sugiyama Haruhito 
Published: September 2020

Abstract
Background: Osimertinib, a third-generation epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI), can be used as second-line treatment for lung cancer patients harboring the T790M substitution. Although osimertinib is more effective than the first-generation EGFR-TKIs used for first-line treatment, its efficacy with respect to long-term patient survival remains unclear even upon the administration of a complete sequence of EGFR-TKI therapy. Moreover, limited information is available regarding genetic diagnostic approaches after the treatment of EGFR-TKI–naïve patients. This study investigated the clinical characteristics of EGFR -mutated lung cancer patients harboring the T790M substitution resistant to EGFR-TKIs, as well as the advantages of rebiopsy and liquid biopsy for these patients. Methods: The medical records of patients screened for EGFR mutations were reviewed. Upon failure of naïve treatment with EGFR-TKIs, except for osimertinib, single-plexus cobas version 2 was repeatedly used to detect the T790M substitution in EGFR via tissue or liquid biopsy. Results: From April 2016 through May 2019, 113 patients were found to harbor EGFR mutations. Sixty patients were treated with EGFR-TKIs, among whom 46 underwent tissue or liquid biopsy. Twenty-nine of these 46 (63%) patients harbored the T790M substitution. In total, 141 rebiopsies were performed. The T790M substitution was detected in 24 of 43 tissue biopsies and 11 of 98 liquid biopsies. If patients displayed an EGFR exon 19 deletion, had a new lesion, and were administered gefitinib as first-line therapy, they were suspected to harbor the T790M substitution. Furthermore, the T790M substitution was detected through rebiopsy in patients with coexisting original mutations, brain metastases, tumor enlargement by ≥ 12 mm, or metastases at minor sites. Conclusion: Among patients with positive factors associated with the T790M mutation, repeated tissue or liquid biopsies are useful to maximize the detection rate of the T790M substitution. Furthermore, these biopsies need to be repeated numerous times in order to reduce “detection overlook” among such patients.
Implementation fidelity of Iron and Folic Acid supplementation (IFAs) and associated challenges among pregnant women in rural Uasin Gishu County Kenya
Authors: Riang'a Roselyter Monchari , Nangulu Anne Kisaka , Broerse Jacqueline E.W. 
Published: September 2020

Abstract
Background Implementation fidelity which is defined as the degree to which programmes are implemented as intended is one of the factors that affect programme outcome, thus requiring careful examination. This study aims to acquire insight into the degree to which nutritional counselling and Iron and Folic Acid supplementation (IFAs) policy guidelines during pregnancy have been implemented as intended and the challenges to implementation fidelity. Methods Data were collected in rural Uasin Gishu County in the western part of Kenya through document analysis, questionnaires among intervention recipients (n=188) and semi-structured interviews with programme implementers (n=6). Data collection and analysis were guided by an implementation fidelity framework. We specifically evaluated adherence to intervention design (content, frequency, duration and coverage), exposure or dosage quality of delivery and participant responsiveness. Results Coverage of nutritional counselling and IFAs policy is widespread. However, partial provision was reported in all the intervention components. Only 10% accessed intervention within the first trimester as recommended by policy guidelines, only 28% reported receiving nutritional counselling, only 18% and 15% of the respondents received 90 or more iron and folic acid pills respectively during their entire pregnancy period, and 66% completed taking the IFAs pills that were issued to them. Late initial bookings to antenatal care, drug stock shortage, staff shortage and long queues, confusing dosage instructions, side effects of the pills and issuing of many pills at one go, were established to be the main challenges to effective implementation fidelity. Anticipated health consequences and emphasis by the health officer to comply to instructions were established to be motivations for adherence to nutritional counselling and IFAs guidelines. Conclusions Implementation fidelity of nutritional counselling and IFAs policy in Kenya is generally weak. There is need for approaches to enhance early access to interventions, enhance stock availability, provide mitigation measures for the side effects, as well as intensify nutritional counselling to promote the consumption of micronutrient-rich food sources available in the local environment to substitute for the shortage of nutritional supplements and low compliance to IFAs.
Functional polymorphisms and transcriptional analysis in the 5' region of the human serotonin receptor 1B gene (HTR1B) and their associations with psychiatric disorders
Authors: Xia Xi , Ding Mei , Xuan Jin-feng , Xing Jia-xin , Yao Jun , Wu Xue , Wang Bao-jie 
Published: September 2020

Abstract
Background The 5-hydroxytryptamine 1B receptor (5-HT1B) plays an essential role in the serotonin (5-HT) system and is widely involved in a variety of brain activities. HTR1B is the gene encoding 5-HT1B. Genome-wide association studies have shown that HTR1B polymorphisms are closely related to multiple mental and behavioral disorders; however, the functional mechanisms underlying these associations are unknown. This study investigated the effect of several HTR1B haplotypes on regulation of gene expression in vitro and the functional sequences in the 5' regulatory region of HTR1B to determine their potential association with mental and behavioral disorders.MethodsSix haplotypes consisting of rs4140535, rs1778258, rs17273700, rs1228814, rs11568817, and rs130058 and several truncated fragments of the 5' regulatory region of HTR1B were transfected into SK-N-SH and HEK-293 cells. The relative fluorescence intensities of the different haplotypes and truncated fragments were detected using a dual-luciferase reporter assay system.Results Compared to the major haplotype T-G-T-C-T-A, the relative fluorescence intensities of haplotypes C-A-T-C-T-A, C-G-T-C-T-A, C-G-C-A-G-T, and C-G-T-A-T-A were significantly lower, and that of haplotype C-G-C-A-G-A was significantly higher. Furthermore, the effects of the rs4140535T allele, the rs17273700C-rs11568817G linkage combination, and the rs1228814A allele made their relative fluorescence intensities significantly higher than their counterparts at each locus. Conversely, the rs1778258A and rs130058T alleles decreased the relative fluorescence intensities. In addition, we found that regions from -1587 to -1371 bp (TSS, +1), -1149 to -894 bp, -39 to +130 bp, +130 to +341 bp, and +341 to +505 bp upregulated gene expression. In contrast, regions -603 to -316 bp and +130 to +341 bp downregulated gene expression. Region +341 to +505 bp played a decisive role in gene transcription.Conclusions HTR1B 5' regulatory region polymorphisms have regulatory effects on gene expression and potential correlate with several pathology and physiology conditions. This study suggests that a crucial sequence for transcription is located in region +341~+505 bp. Regions -1587 to -1371 bp, -1149 to -894 bp, -603 to -316 bp, -39 to +130 bp, and +130 to +341 bp contain functional sequences that can promote or suppress the HTR1B gene expression.
QTL Mapping of Agronomic and Economic Traits for Four F2 Populations in Upland Cotton
Authors: Li Hongge , Pan Zhaoe , He Shoupu , Jia Yinhua , Geng Xiaoli , Chen Baojun , Wang Liru , Pang Baoyin , Du Xiongming 
Published: September 2020

Abstract
Background: Upland cotton (Gossypium hirsutum) accounts for more than 90% of annual world cotton output due to its high yield potential. However, yield traits and fiber quality traits exhibit negative correlations in most cases. Here, we constructed four F2 populations, using two normal lines and two introgression lines, for simultaneously detection the genetic basis underlying complex traits such as yield and fiber quality in upland cotton. Subsequently, the phenotyping of 8 agronomic and economic traits along with quantitative trait loci (QTL) mapping was implemented. Results: Extensive phenotype variations and transgressive segregation were found across segregation populations. Four genetic maps were constructed with the length of 585.97cM, 752.45cM, 752.45cM and 1163.66cM. The mapping resulted in the identification 50 QTLs (27 were for fiber quality traits and 16 for yield traits) across four populations. Multiple QTLs having the common maker, such as qBW4 and qBW2, or residing in the same QTL cluster, such as qLP9 and qFL9-1, were prioritized for further research. Conclusions: These findings will provide insight into the genetic basis of simultaneous improvement of yield and fiber quality in upland cotton breeding.