bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gastric cancer cell proliferation is inhibited by α-santonin via targeting of PI3K and AKT activation
Authors: Nie Lin , Zhang Lijiu 
Published: June 2020

Purpose: To investigate the effect of α-santonin on proliferation of gastric cancer cells.Methods: Cell proliferation was analysed by 3-4-5-Dimethylthiazol-2-yl-25-diphenyltetrazolium bromide (MTT) assay and migration by wound healing assay. Matrigel coated Transwell chamber was used for determination of cell invasion. Expression of proteins and mRNA was assessed using western blot and RT-PCR assay, respectively.Results: In NUGC4 and MKN45 cell cultures, treatment with α-santonin promoted miR 145 expression significantly when compared to control. Treatment of NUGC4 cells with α-santonin for 48 h significantly increased apoptosis in comparison to control. At 100, 150 and 200 μM concentrations of α-santonin, the level of cell apoptosis increased to 45, 53 and 64 %, respectively (p < 0.05). Treatment with α-santonin caused NUGC4 cell population increase in G1/G0 phase with reduction in S and G2/M phases. A significant reduction in NUGC4 cell invasion was observed following treatment with α-santonin. The α- santonin treatment of NUGC4 cells at 200 μM concentration markedly reduced cell invasion (p < 0.05). Treatment of NUGC4 cells with α-santonin reduced the expression of c Myc, PI3K, and p AKT. The production of MMP-2 and MMP-9 in NUGC4 cells was also decreased by α-santonin treatment.Conclusion: The study demonstrates that α-santonin plays important role in inhibition of gastric cancer cell proliferation by arrest of cell cycle and apoptosis induction. Moreover, the activation of PI3K and AKT was also suppressed by α-santonin. Therefore, α-santonin can potentially be used for the treatment of gastric cancer.
Keywords: Apoptosis, MicroRNA, Tumor suppressor, Metastasis, Infiltration
Metformin exerts renoprotective effect by reducing proteinuria in spontaneously-hypertensive rats
Authors: Liu Yuanyuan , Wang Chengjian , Zhang Enyuan 
Published: June 2020

Purpose: To study the mechanism involved in metformin (DMBG)-induced reduction of albuminuria in spontaneously-hypertensive rats (SHRs).
Methods: The SHRs were divided into DMBG SHR group (n = 30) given DMBG (300 mg/kg) and NC SHR group (n = 10) given distilled water (2 ml/day), while Wistar-Kyoto rats (WKY) rats were divided into DMBG WKY group (n = 30) given DMBG (300 mg/kg ) and NC WKY group (n = 10) administered distilled water (2 ml/day). Urinary protein levels were measured and compared. Inflammatory infiltration in renal tissue, and expression of vascular endothelial growth factor (VEGF) were assessed. Abdominal aortic blood was used to measure serum levels of creatinine and blood urea nitrogen (BUN).
Results: Urinary protein was markedly elevated in SHRs, while BUN in SHRs was lowered, relative to WKY rats (p < 0.05). Moreover, inflammatory cell population was lower in DMBG SHRs group than in WKY rats (p < 0.05). Kidney expressions of VEGF in SHR and WKY rats treated with metformin were significantly reduced, relative to those in untreated rats, but VEGF-A in DMBG SHR rats was markedly elevated, relative to NC SHR group, and was also raised in DMBG WKY, when compared with NC WKY rats (p < 0.05).
Conclusion: Metformin reduces albuminemia in spontaneously hypertensive rats by up-regulating VEGF expression, thereby exerting renoprotective effect. This finding provides ideas for research and development of other therapeutic approaches.
Keywords: Metformin, Spontaneous hypertension, Renoprotective, Albuminuria, Kidney, Vascular endothelial growth factor (VEGF)
Effect of <i>Evodia rutaecarpa</i> (Juss) Benth extract on Alzheimer disease in mice
Authors: Cai Ang , Xiao Liu , Zhou Yan-Ping , Zhang Zhi-Guo , Yang Quan-Wei 
Published: June 2020

Purpose: To investigate the protective effect of Evodia rutaecarpa (Juss.) Benth. extract (ERBE) against Alzheimer's disease in 3xTg-AD mice.
Methods: The cognitive function of 3xTg-AD mice was assessed using Morris water maze test. The levels of amyloid beta deposits and NeuN in the mouse hippocampus were evaluated by immunohistochemistry. Brain neurotrophic derived factor (BDNF) and tyrosine kinase B (TrkB) expressions were determined by western blot analysis.
Results: ERBE treatment significantly ameliorated learning and memory deficits in AD mice, as shown by increased time spent in the target zone during probe tests. The escape latency in the animals treated with 400 mg/kg ERBE (20.5 ± 1.3 s) was significantly higher than untreated 3xTg-AD mice (12.4 ± 1.3 s, p < 0.01). In addition, ERBE significantly decreased Aβ deposits, increased NeuN-positive cells, and upregulated the expressions of BDNF (1.4 ± 0.2, p < 0.05) and TrkB (1.1 ± 0.2, p < 0.05) in 3xTg AD mice.
Conclusion: The results suggest that ERBE administration may be a useful strategy for treating memory impairment induced by several neurodegenerative diseases.
Keywords: Evodia rutaecarpa, Alzheimer, Memory impairment, NeuN-positive cells
Anti-diabetic effect of the polyphenol-rich extract from <i>Tadehagi triquetrum</i> in diabetic mice
Authors: Lin Xiaowan , Zhou Xinxin , Sun Wanying , Zhang Lu , Zhang Caiyun , Zhang Xiaopo 
Published: June 2020

Purpose: To clarify the diabetes-reducing abilities of the polyphenol-rich extract from Tadehagi triquetrum (HC) in diabetic ob/ob mice.Methods: Aerial parts of T. triquetrum were extracted under reflux and partitioned by n-butanol to generate HC. The effects of HC consumption on blood glucose and lipids, insulin resistance, and liver glucose metabolism were evaluated in vivo. The main compounds of HC were tested for their effects on stimulating glucose consumption and uptake by HepG2 hepatocytes and C2C12 myotubes.Results: After HC treatment, body fat, subcutaneous fat, and epidydimal fat masses decreased (p <0.05), while mean daily food intake was unaffected. HC (200–400 mg/kg) decreased fasting blood glucose, glycosylated serum protein (GSP), and glycosylated hemoglobin (HbAlc); it also lowered hyperinsulinemia, improved oral glucose tolerance, and reduced hyperlipidemia and liver fat content (p < 0.05). HC treatment markedly elevated liver glycogen content and activity of hepatic glucokinase and pyruvate kinase (p < 0.05). Eight polyphenols were isolated from HC, six of which potently stimulated glucose consumption and uptake in vivo.Conclusion: HC has potent antidiabetic activities. Polyphenols are the main compounds accounting for these effects. Chronic oral administration of HC may be an alternative therapy for managing diabetes, but this has to be subjected first to clinical studies.
Keywords: Tadehagi triquetrum, Diabetes, Phenylpropanoid glucosides, Pyruvate kinase, Glucokinase
Ultra-performance liquid chromatography–tandem mass spectrometric determination of ramipril in human plasma
Authors: Abdel-Gawad Sherif A. 
Published: June 2020

Purpose: To develop a sensitive and accurate ultra-performance liquid chromatography–tandem mass spectrometric (UPLC-MS) method for quantification of ramipril in human plasma.Methods: Ramipril was extracted from biological fluid using equal volumes of n-hexane and propanol (1:1, v/v), and then chromatographed in a suitable C18 column with methanol: 0.1 % HCOOH (4: 1, v/v) as mobile phase. Atorvastatin was used as an internal standard for the chromatographic separation and quantification. The method was validated according to the United States Food and Drug Administration guidelines for standard indices.Results: Ramipril was determined in the concentration range 0.05 and 1000 ng/mL the validation procedure exhibited a correlation coefficient of 0.9979 + 0.002 (p = 0.05). The studied drug was quantified with lower ceiling of 0.05 ng/mL, and showed an accuracy of 105.00 %.Conclusion: A sensitive UPLC-MS analytical method has been successfully developed for the quantification of ramipril in human plasma. This method can be applied efficiently for the quantification of ramipril in bioavailability and pharmacokinetic studies.
Keywords: Liquid chromatography–tandem mass, Ramipril, Stability, Biological fluids, Plasma
MiR-624-5p enhances cell resistance against cisplatin via PDGFRA/Stat3/PI3K axis in ovarian cancer
Authors: I-Ju Lin , YongJie Tian 
Published: June 2020

Purpose: The purpose of this study was to evaluate the role of miR-624-5p in ovarian cancer.Methods: MiR-624-5p expression in ovarian cancer {OC) cell lines and normal cells (NCs) was evaluated and compared the differential miR-624-5p in OC A2780 cells and cisplatin-resistant OC cell line (A2780/DDP). CCK-8 was used to evaluate changes in cell viability of the A2780 and A2780/DDP cell lines as well as silenced miR-624-5p. Western Blot examined the Stat3 and phosphorylated Pi3k. The binding between PDGFRA and miR-624-5p was predicted on Targetscan and verified through Luciferase Reporter Assay. The role of PDGFRA in A2780/DDP by overexpressing PDGFRA was evaluated by RT-qPCR and CCK-8 assays. RT-qPCR assay also measured miR-624-5p expression responsive to different dosages of cisplatin and CCK8 examined viability levels correspondingly. In addition, the interplay of PDGFRA and miR-624-5p by combined downregulation of both miR-624-5pand PDGFRA were evaluated.Results: OC cells had higher miR-624-5p expression than NCs but lower compared to cisplatinresistant A2780/DDP cells. A2780/DDP cells had higher viability than OC cell line A2780. Stat3 and phosphorylated PI3K were activated in A2780/DDP cells. Silencing miR-624-5p led to lower viability inA2780/DDP cells. miR-624-5p expression dropped as the cisplatin concentration increased, resulting in decreasing viability respectively. Luciferase Reporter assay validated the binding of miR-624-5p and PDGFRA in A2780/DDP cells. Overexpressed PDGFRA induced lower cell viability in A2780/DDP cells. Downregulation of PDGFRA partially restored the lowered viability and inhibited Stat3 as well as phosphorylated Pi3k induced by miR-624-5p inhibitor.Conclusion: MiR-624-5p could add to the cellular resistance to cisplatin in OC in-vitro model, which indicated that it might help unveil the mystery of drug-resistance in clinical stage of ovarian cancer.
Keywords: MiR-624-5p, resistance, cisplatin, PDGFRA/Stat3/PI3K, ovarian cancer
Cytochrome P450 expression-associated multiple-hit pathogenesis of non-alcoholic fatty liver disease (NAFLD) in HepG2 cells
Authors: Sukkasem Nadta , Chatuphonprasert Waranya , Jarukamjorn Kanokwan 
Published: June 2020

Purpose: To establish a free fatty acid (FFA)-induced non-alcoholic fatty liver disease (NAFLD) model in HepG2 cells.Methods: HepG2 cells were incubated with 0.1, 1, or 5 mM oleic acid (OA) or palmitic acid (PA) for 24 h. Histological features were examined by oil-red-O staining. Expression levels of metabolic genes (peroxisome proliferator activated receptors α/γ, sterol regulatory element binding proteins 1a/1c, acetyl-CoA carboxylase, acyl-CoA oxidase, and fatty acid synthase), antioxidative genes (catalase and superoxide dismutases 1/2), and cytochrome P450 genes (CYP1A2, CYP2C19, CYP2D6, CYP2E1, CYP3A4, and CYP4A11) were determined by reverse transcription-real time polymerase chain reaction (RT-qPCR).Results: Intracellular lipid storage was observed in cells treated with 1 mM OA or PA while cell shrinkage was present at 5 mM concentrations of both. Expression of all metabolic genes were elevated by 1 mM PA and 5 mM OA and PA. Expression of all antioxidative genes were diminished by 5 mM OA whereas 5 mM PA only reduced superoxide dismutase-2 expression. Expression of CYP1A2, CYP2D6, and CYP3A4 genes were down-regulated by both FFAs, CYP2C19 was induced by PA, while CYP2E1 and CYP4A11 were up-regulated in a concentration-dependent manner.Conclusion: PA was the more potent steatogenic agent in an OA- or PA- induced NAFLD model in HepG2 cells. Increase in intracellular hepatic lipid and expression of metabolic genes, suppression of antioxidative genes, suppression of CYP1A2, CYP2D6, and CYP3A4, and induction of CYP2E1 andCYP4A11 correlated with the multiple-hit pathogenesis model of NAFLD. These findings suggest that PA-induced NAFLD model in HepG2 cells is a suitable in vitro model for studying novel therapeutic approaches to NAFLD treatment.
Keywords: NAFLD, Multiple-hit pathogenesis, Free fatty acid, Oleic acid, Palmitic acid
Protective effects of 4, 5-O-dicaffeoylquinic acid against mouse sepsis via down-regulation of TNF-α, IL-6 and IL-8
Authors: Wang XiaoBo , Wu JianHua , Ni BuKao 
Published: June 2020

Purpose: To investigate the protective effects of 4, 5-O-dicaffeoylquinic acid (DCQA) isolated from Xanthium sibiricum Patr. against mouse sepsis caused by cecal ligation/puncture (CLP) in vivo, as well as the molecular mechanisms of action involved.Methods: DCQA (7.5, 15, and 30 mg/kg/day) were administered to the mice with sepsis and the survival rate was obtained. Tumor necrosis factor (TNF)-α and interleukin (IL)-6 were examined by enzyme-linked immunosorbent assay (ELISA). Subsequently, the lipopolysaccharide (LPS) neutralizing ability of DCQA (2, 4, and 8 μg/mL) was measured using limulus amebocyte lysate (LAL) test in vitro. Furthermore, the effect of DCQA (10, 20, and 40 μg/mL) on mRNA expression of TNF-α, IL-6, and IL-8 in LPS (100 ng/mL)-treated RAW 264.7 cells was assessed using quantitative real time-polymerase chain reaction (RT-qPCR) assay.Results: DCQA significantly improved the survival rate of mice with sepsis caused by CLP (35, 50, and 65 %, respectively vs. 15 % for control, p < 0.05). LPS levels fell on co-incubation with DCQA in vitro. Moreover, ELISA and RT-qPCR results revealed that DCQA treatment lowered tendency in the mRNA expression of TNF-α, IL-6, and IL-8 (p < 0.01).Conclusion: DCQA exhibits protective effects against sepsis in mice mediated by downregulating TNF-α, IL-6, and IL-8. Further studies, in animals and humans are requied to determine the safety and efficacy of DCQA in both animal and clinical management of sepsis.
Keywords: Xanthium sibiricum, 4,5-O-Dicaffeoylquinic acid, Sepsis, Cecal ligation and puncture, Lipopolysaccharide, TNF-α, IL-6, IL-8
Anhuienoside C ameliorates atherosclerosis in rats via regulation of the NFκB/eNOS/NO signaling pathway
Authors: Xu Xianghua , Ma Qiang , Qin Hao , Zhang Junbo , Zhang Bo , Pang Honggang 
Published: June 2020

Purpose: To investigate the protective effect of anhuienoside C (AC) against high cholesterol dietinduced atherosclerosis in a rat model.
Methods: Atherosclerosis was induced in rats by administration of high fat diet for 8 weeks, and AC (20 and 40 mg/kg) was administered orally. The effect of AC was determined by assessing serum lipid profiles and mediators of inflammation, as well as oxidative stress parameters in aortic tissue using enzyme-linked immunosorbent assay (ELISA). Western blot assay and reverse transcription polymerase chain reaction (RT-PCR) were used for the evaluation of protein expressions.
Results: Serum levels of total cholesterol (TC), triglycerides (TGs), high density lipoprotein (HDL), low density lipoprotein (LDLP), and IL-1β, IL-18, TNF-α and NF-κB were significantly reduced in AC-treated group, relative to atherosclerotic rats (p < 0.01). Moreover, parameters of oxidative stress were attenuated in the aortic tissues of AC-treated group, when compared with atherosclerotic rats. There was significant increase in eNOS expression, and marked decrease in the expressions of MAPK and NF-kB protein in the aortic tissue homogenate of AC treated group, relative to atherosclerotic group (p <0.01). Treatment with AC attenuated the mRNA expressions of ICAM-1, VCAM-1 and MCP-1 in aortic tissue of the atherosclerotic rats.
Conclusion: These results reveal that AC prevents atherosclerosis in rats by modulating the NFκB/eNOS/NO signaling pathway, and thus, can thus potentially be developed as anti-atherosclerotic agent.
Keywords: Anhuienoside C, Atherosclerosis, Inflammation, Oxidative stress, Cholesterol
Pengembangan Desa Wisata Pentingsari, Kabupaten Sleman dalam Perspektif Partisipasi Masyarakat
Authors: Wahyuni Dinar 
Published: June 2020

Village tourism is one form of tourism that applies the concept of community empowerment so that community participation is the most important component in its development. This study aims to describe the development of the Pentingsari Tourism Village in the perspective of community participation. This research is descriptive with a qualitative approach. The results showed that the development of the Pentingsari Tourism Village received full support from the Pentingsari community and local government through its participation in tourism activities. At the planning and decisionmaking stages, the community participated from planning, socialization to community and village government, to making decisions about the formation of a tourism village. Community participation in the implementation phase is carried out by giving thought, material, and being directly involved in every tourism village development activity and trying to create a tourism village that is ready to compete in the tourism industry. Participation in the stage of enjoying the results is shown by improving the living conditions of the Pentingsari community due to tourism activities. This means that the community enjoys the results of tourism, economically, socially, culturally, and environmentally. Furthermore, community participation in the evaluation phase was demonstrated through their involvement in regular meetings between village tourism managers, village government and local government.AbtrakDesa wisata merupakan salah satu bentuk wisata yang menerapkan konsep pemberdayaan masyarakat sehingga partisipasi masyarakat menjadi komponen terpenting dalam pengembangannya. Penelitian ini bertujuan untuk mendeskripsikan tentang pengembangan Desa Wisata Pentingsari dalam perspektif partisipasi masyarakat. Penelitian ini bersifat deskriptif dengan pendekatan kualitatif. Hasil penelitian menunjukkan bahwa pengembangan Desa Wisata Pentingsari mendapat dukungan penuh dari masyarakat Pentingsari dan pemerintah daerah melalui partisipasinya dalam kegiatan wisata. Pada tahap perencanaan dan pengambilan keputusan, masyarakat berpartisipasi mulai dari perencanaan, sosialisasi ke masyarakat dan pemerintah desa hingga pengambilan keputusan tentang pembentukan desa wisata. Partisipasi masyarakat dalam tahap implementasi dilakukan dengan memberikan pemikiran, materi, dan terlibat langsung dalam setiap kegiatan pengembangan desa wisata serta berupaya menciptakan desa wisata yang siap bersaing di industri pariwisata. Partisipasi dalam tahap menikmati hasil ditunjukkan dengan peningkatan kondisi kehidupan masyarakat Pentingsari akibat kegiatan wisata. Hal ini berarti bahwa masyarakat menikmati hasil dari kegiatan wisata baik secara ekonomi, sosial, budaya, dan lingkungan. Selanjutnya partisipasi masyarakat dalam tahap evaluasi ditunjukkan melalui keterlibatannya dalam pertemuan rutin antarpengelola desa wisata, pemerintah desa, dan pemerintah daerah setempat.
Satellite-retrieved sea ice concentration uncertainty and its effect on modelling wave evolution in marginal ice zones
Authors: Nose Takehiko , Waseda Takuji , Kodaira Tsubasa , Inoue Jun 
Published: June 2020

Abstract. Ocean surface waves are known to decay when they interact with sea ice. Wave–ice models implemented in a spectral wave model, e.g. WAVEWATCH III® (WW3), derive the attenuation coefficient based on several different model ice types, i.e. how the model treats sea ice. In the marginal ice zone (MIZ) with sea ice concentration (SIC) < 1, the wave attenuation is moderated by SIC: we show that subgrid-scale processes relating to the SIC and sea ice type heterogeneity in the wave–ice models are missing and the accuracy of SIC plays an important role in the predictability. Satellite-retrieved SIC data (or a sea ice model that assimilates them) are often used to force wave–ice models, but these data are known to have uncertainty.
To study the effect of SIC uncertainty ΔSIC on modelling MIZ waves during the 2018 R/V Mirai observational campaign in the refreezing Chukchi Sea, a WW3 hindcast experiment was conducted using six satellite-retrieved SIC products based on four algorithms applied to SSMIS and AMSR2 data. The results show that ΔSIC can cause considerable wave prediction discrepancies in ice cover. There is evidence that bivariate uncertainty data (model significant wave heights and SIC forcing) are correlated, although off-ice wave growth is more complicated due to the cumulative effect of ΔSIC along an MIZ fetch. The analysis revealed that the effect of ΔSIC can overwhelm the uncertainty arising from the choice of model ice types, i.e. wave–ice interaction parameterisations. Despite the missing subgrid-scale physics relating to the SIC and sea ice type heterogeneity in WW3 wave–ice models – which causes significant modelling uncertainty – this study found that
the accuracy of satellite-retrieved SIC used as model forcing is the dominant error source of modelling MIZ waves in the refreezing ocean.
Silicate and Oxide Mineral Chemistry and Textures of the Norilsk-Talnakh Ni-Cu-Platinum Group Element Ore-Bearing Intrusions
Authors: Schoneveld Louise , Barnes Stephen J. , Williams Morgan , Vaillant Margaux Le , Paterson David 
Published: June 2020

AbstractA large proportion of the disseminated sulfide ores of the Norilsk-Talnakh camp are hosted within olivine-rich, ultramafic cumulate layers called picro-gabbrodolerite units. In this study we quantitatively analyze the chemistry and textures of the silicate and oxide minerals within olivine-bearing cumulates of the Kharaelakh, Norilsk 1, and Talnakh intrusions to determine how these intrusions compare to each other and to establish the liquidus phase assemblage and crystallization sequence and how the liquid component evolved during solidification.Crystal size distributions indicate that much of the olivine and clinopyroxene oikocrysts grew together in situ as the first of the cumulus phases at contrasting growth rates. These large clinopyroxene oikocrysts record a significant drop in Cr in the system by a significant decrease in Cr content of the outer rims compared to the cores. The chadacrysts of olivine and spinel within the clinopyroxene record the chemistry of the first stages of crystallization, while the minerals in the framework of the cumulate show a relative reduction in Cr and enrichment in incompatible elements such as Ti, Zn, Y, and the rare earth elements, indicative of the enrichment through reactions with the trapped liquid during postcumulate growth.Due to the entrapment of the olivine and spinel in rapidly growing clinopyroxene, these minerals record a history of the changing chemistry during cumulate and postcumulate growth, giving us an insight into the changing conditions during the solidification of intrusions.