bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical implication of metabolic syndrome in nonobese patients with antineutrophil cytoplasmic antibody-associated vasculitis
Authors: soobinl22@naver.com Soo Bin , Kwon Hyeok Chan , Pyo Jung Yoon , Kang Mi Il , Song Jason Jungsik , Park Yong-Beom , Park Jun Yong , Lee Sang-Won 
Published: May 2020

Abstract
Objective: We investigated the prevalence of metabolic syndrome (MetS) in all or nonobese patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) and compared it with age- and gender-matched controls. Also, we assessed the effect of variables at diagnosis on the risk of cardiovascular disease (CVD) in all or nonobese AAV patients. Methods: In this study, 173 AAV patients and 344 controls were included and MetS was defined by the National Cholesterol Education Program Adults Treatment Panel III criteria. The obesity based on BMI was defined as BMI ≥ 25 kg/m 2 . The follow-up duration was defined as the period from diagnosis to the last visit or to each poor outcome occurrence. Results: The median age of AAV patients was 58.7 years and 57 patients were men. The prevalence of MetS was 50.9% in all AAV patients and 46.5% in nonobese AAV patients, which were significantly higher than 37.8% in all controls and 28.2% in nonobese controls. In the Kaplan-Meier survival analysis, Mets at diagnosis significantly reduced the cumulative CVD-free survival rate in both all and nonobese AAV patients. In the multivariable Cox hazards model analysis, CVD during follow-up was significantly associated with both BVAS (HR 1.159) and MetS at diagnosis (HR 9.036) in nonobese AAV patients. Conclusions: The prevalence of MetS at diagnosis in all or nonobese AAV patients was significantly higher than those in all or nonobese controls. Furthermore, both BVAS and MetS at diagnosis increased the risk of CVD in nonobese AAV patients.
The Transition in Intensive Care Unit Patients: A Concept Analysis
Authors: Ghorbanzadeh Kobra , Ebadi Abbas , Hosseini Mohammadali , Madah Sadat , Khankeh hamidreza 
Published: May 2020

Abstract
Background: Transition in the health system is associated with the movement of the patient between clinical units and between hospitals, This is a complex process with several potential challenges including medical errors, adverse events, increased costs and patient dissatisfaction. Evidence shows that there is a need for greater clarity regarding the concept of transition. Objectives: The present Study was conducted to clarify the core elements of transition in patients admitted to the ICU.Methods: Walker and Avant's eight-step model was used to guide this concept analysis to provid a comprehensive definition of transition. A Literature search was conducted on CINAHL, Scopus, Pubmed, and Google scholar using the following keywords: transition, intensive care unit, transition care, patient transfer and transition process. Thus, after an extensive review of resources published in the years 2000-2020, articles related to this concept were examined based on the inclusion criteria. Definitions, properties, applications, consequences and empirical references of the concept of transition were extracted.Results: Transition is a complex, multifaceted concept with defining attributes: Critical points and events, disturb normal life, passage of change, instability and un-anticipated changes, Multiple needs, Inadequate continuity of care, Poor coordination of care and communication among health care providers, patients and families, multiple factor, multiple professionals, Awareness, Engagement And Adaptation. The differences in the response to transition according to the patient's condition or position (beliefs, attitudes, socioeconomic status, willingness and knowledge) and environmental conditions (the status of communication and support from the community) lead to the development of confidence, adaptation, recovery from critical illness and return to normal life or disability and other complications.Conclusion: The transition is a process of progressive change and the adaptation requires training and environmental changes to improve and develop new skills for transition. Nurses play a supportive and complementary role in successful transition of patients. Therefore, studies need to focuse on the evaluation of nurses' perceptions of patient transition and the consequences and outcomes. The result of this study provide a definition of transition that is relevant and useful for clinical research and practice in healthcare setting.
Discovery of New Mutation Sites of KIF21A Gene in Chinese Patients with Congenital Fibrosis of the Extraocular Muscles
Authors: Xia Cuiran , Shi Ruilan , Nan Lianghuan , Hao Jing , Jia Yading , Li Danjie 
Published: May 2020

Abstract
Background Congenital fibrosis of the extraocular muscles (CFEOM) is a rare hereditary nonprogressive disorder characterized by bilateral ptosis, which shows severely limited ocular motility. We reported a new mutation site of KIF21A gene in a Chinese family with congenital fibrosis of the extraocular muscles type 1 (CFEOM1).Methods A retrospective study of case series was conducted in this study. Standard ocular examinations were performed on 10 family members in a CFEOM1 family. Next-generation sequencing (NGS) was performed to sequence 828 genes related to oculopathy. The splice sites of the KIF21A gene were analyzed to detect the existence of mutations.Results The five patients with CFEOM1 were found in the family members of a Chinese family on three generations. A new KIF21A pathogenic SNP mutation site KIF21A-ex20 c.2821C > T (p.Arg941Trp) was identified by comparing with human genome reference genes and Sanger sequencing. .Conclusions A new KIF21A pathogenic SNP mutation site KIF21A-ex20 c.2821C > T (p.Arg941Trp) could possibly be a major disease‑causing gene for the Chinese family with CFEOM1.
The Impact of Expanded Insurance Coverage of Dental Prosthetic Services on Dental Care Utilization Among Older Adults in South Korea: Based on the Korean Health Panel Survey.
Authors: Choi Jin-Sun , Jung Se-Hwan 
Published: May 2020

Abstract
Background: In Korea, the National Health Insurance Service (NHIS) began its coverage of dentures and dental implants for older people in 2012 and 2014, respectively. This study aimed to investigate the impact of these policies on dental care utilization among people aged 65 years or older according to their sociodemographic characteristics. Methods: Data was collected from the Korea Health Panel (KHP; years 2012 and 2015). The statistical significance of the relationships between sociodemographic characteristics and use of outpatient dental care, denture, and dental implant were analyzed. Results: Results showed an increase of 5.7%, 1.4%, and 2.8% for use of outpatient dental care, denture, and dental implant, respectively, over the course of three years. Including dentures increased its use by 2.5–3.7 times among people aged 70 years or older. Including dental implants alleviated the disparities among age groups and duration of education, except among uneducated people; however, it caused inequity according to household income. Conclusions: Thus, some Korean older adults remain neglected from the benefits of the expanded NHIS; the NHIS should aim for the provision of universal health coverage, and older adults’ access to dental care should be enhanced by the implementation of policies to promote oral health care utilization, Dental prosthetic services, Older adults, Insurance coverage
Inhibiting mir-29 and targeting ADAM12: lidocaine inhibits breast cancer development
Authors: Feng Gui , He Fei 
Published: May 2020

Abstract
Breast cancer is the most common cancers among women in the world. For hundreds of years, researchers are devoted for developing new strategy against cancer. As a rapid and effective local anesthetic, lidocaine is reported having multiple-physiological functions in clinic treatment, such as anti-cancer and anti-inflammatory activity. Besides, the microRNAs (miRNAs) have been demonstrated to be involved in the cancer development, and the miRNA-29 family is abnormally expressed in a variety of cancers, which could not only regulate the cancer cell proliferation, migration and invasion, but also promote cancer cell apoptosis by binding to target proteins. However, the protective effect of lidocaine on breast cancer cells and the mechanism was still unclear. In the present study, the relative expression level of the miRNA-29 in cancer cells and tissues was measured with quantitative RT-PCR. Bioinformatic analysis was performed to predict the potential target of the miRNA-29 in breast cancer cells, and the luciferase reporter assay was employed to validate the direct binding of the target protein and the miRNA-29 in breast cancer cells. Cell Counting Kit-8 (CCK-8) and the Cell Apoptosis Assay Kit were utilized to analyze the cancer cell proliferation and apoptosis after lidocaine treatment.
Senior house officer perspectives on the educational benefits of a local teaching programme at a specialist orthopaedic hospital
Authors: Kyriacou Steven , Eastwood Deborah 
Published: May 2020

Abstract
Background:

Delivering local teaching programmes equally relevant to all senior house officer (SHO) equivalent junior doctors within a tertiary level specialist hospital is challenging. This study aimed to establish the views of SHOs within a specialist orthopaedic hospital on the value of the current local teaching programme to their educational needs how it may potentially be improved?
Methods:

Data was collected via questionnaires of SHOs selected through a combination of convenience and purposive sampling. Responses underwent thematic analysis which utilised a hybrid model of a priori and emergent coding.
Results:

12 (50%) of SHOs within the hospital responded. Thematic analysis of completed questionnaires resulted in identification of 4 main themes: quality/utility of teaching, content of teaching, accessibility of teaching and non-educational benefits. Feedback received regarding the existing local teaching programme was largely positive. Opinions on the utility and content of the teaching programme were found to be the most varied.
Conclusion:

This study highlights the difficulties in delivering a teaching programme to a clinical learner group with diverse educational needs in a specialist hospital setting. Such issues may be minimised by learner survey to identify educational topics of equivalent utility to the majority of the learner group.
Bone Markers in Charcot Neuroarthropathy
Authors: Patel Komal , Bernstein Brent , Grahn Brandy , Stoltzfus Jill 
Published: May 2020

Abstract
Background

There have been multiple studies evaluating the effectiveness of pharmaceuticals in the treatment of acute Charcot neuroarthropathy (CNA). The effectiveness of most pharmaceuticals historically has been based on the levels of certain biomarkers of bone turnover. Previous research has shown bone turnover markers are increased in acute phase of CNA. However, biomarkers activity in chronic phase of CNA and its utility for monitoring treatment response remains to be established. In this article, we evaluated the relationship of such biomarkers to disease severity. We hypothesized that biomarkers such as BSAP and DPD:CRT levels will be directly proportional to disease severity i.e. increased in acute CNA and decreased in chronic CNA. Hence pharmacologic treatment in acute CNA with reference to levels of such biomarkers potentially could be beneficial in clinical settings.
Methods

We retrospectively reviewed 41 patients diagnosed with acute and chronic CNA in our Charcot clinic. Disease severity was determined via pedal temperature difference between affected and unaffected limbs using an OMEGA Surface Temperature Scanner in conjugation with radiographic films and clinical presentation. Temperature of 2 degrees Celsius or greater is considered acute CNA, temperature less than 2 degree Celsius is considered chronic CNA. Urine and serum blood samples were collected at baseline to evaluate the levels of BSAP and DPD:CRT via immunoassays. Statistical analysis was performed via separate Mann Whitney rank sums tests to determine between-group differences in BSAP and DPD:CRT in acute versus chronic CNA.
Results

Bone formation marker BSAP (p = 0.46) and bone resorption marker DPD:CRT (p = 0.92) were not significantly different in acute versus chronic CNA. In acute CNA patients (n = 31) median BSAP was 11.9 (range 3.8–41.3) versus 15.2 (range 4.4–40.7) in chronic CNA patients. In acute CNA patients, median DPD:CRT was 7.8 (range 3.1–38.4) versus 8.8 (range 3.3–18.6) in chronic CNA patients.
Conclusion

The lack of significant between-group differences in BSAP and DPD:CRT calls into question their reliability in monitoring pharmaceuticals effectiveness in acute versus chronic CNA.
Peripheral T Lymphocytes Predict the Severity and Prognosis in Patients with HBV-Related Acute-On-Chronic Liver Failure
Authors: Zhang Feng , Wang Feixia , Sun Weiwei , Xiao Qian , Liang Chongfeng , Jiang Shulian , Lian Yanan , Shao Jiangjuan , Tan Shanzhong , Zheng Shi zhong 
Published: May 2020

Abstract
Background Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a life-threatening syndrome with high mortality. Biomarkers are urgently needed to predict the prognosis of HBV-ACLF. Recent evidence suggests a key role for immune system in the pathology of HBV-ACLF. Here, we analyzed the correlation between peripheral blood T lymphocytes and the severity and prognosis in HBV-ACLF patients.Methods Sixty-six patients with HBV-ACLF received conventional medical treatments for four weeks. Twenty-three healthy subjects and 23 HBV patients were enrolled for comparison. We determined white blood cell count, lymphocytes, CD3+, CD4+ and CD8+ T cells, and CD4+CD25+ Treg cells in the blood of all subjects. Their associations with laboratory parameters before or after treatments were statistically analyzed.Results The results showed that compare normal subjects and chronic hepatitis B patients, HBV-ACLF patients had significantly increased white blood count, CD4+ T cells and decreased lymphocytes, CD3+ T cells and Treg cells. Correlation analysis showed that white blood cell, lymphocytes and peripheral T lymphocytes were correlated with PTA and MELD scores. After treatment, white blood cell, lymphocytes, and peripheral T lymphocytes were also correlated with PTA and MELD scores. Additionally, TBIL, ALT, INR, MELD and white blood cell count were potential prognostic criteria for HBV-ACLF patients. In conclusion, HBV-ACLF patients had depletion and dysfunction of immune system. Changes of peripheral T lymphocytes were closely related to the pathogenesis and prognosis of disease.Conclusions Our results may contribute to predict the severity of HBV-ACLF, and provide a prognosis response to improve the treatment of HBV-ACLF.
The effects of workplace health education on smoking-related behaviour and unequal gains by job position in China: ABWMC programme findings
Authors: Lin Haoxiang , Chen Meijun , Chang Chun , Liu Zhao 
Published: May 2020

Abstract
Objective: Although the Chinese government has introduced a series of regulations to promote tobacco-related health education in workplaces, the implementation impact has been far from satisfactory. The aim of the present study was to explore the effects of company level tobacco-related health education and the potential unequal gain across job positions.Methods: Data from the 2018 Asia Best Workplace Mainland China programme were employed to address these aims. This was a cross-sectional study that included 14195 employees from 79 companies in mainland China. Spearman correlation tests were used to examine unadjusted correlation between the study variables, and binary logistic regression was used for multivariable analysis.Results: Tobacco-related health education was associated with better smoking harm awareness, lower second-hand smoke exposure, better perceived workplace environment and positive health information-seeking behaviour. Job position interacted with health education, suggesting that positive effects of health education were smaller for general employees than employees who held an administrative position.Conclusions: We conclude that workplace tobacco-related health education was not only associated with tobacco control effects but also had spillover effects, which were significant for higher-ranking employees. Policy makers should recognize and reduce the potential health disparities.
Impact of cancer breast distance from skin surface on axillary lymph node metastasis in female patients with early breast cancer: correlation between radiological and pathological distance
Authors: Essa Mohamed S , Ahmad khaled S , Zayed Mohamed E , Faheem Mohamed H , Abdalla Rana 
Published: May 2020

Abstract
Background: The aim of this study is to evaluate the relation between proximity of breast cancer to the skin and incidence of axillary nodal metastasis in order to clarify a new guideline in treatment of early cancer breast.Methods: This study includes 50 female patients with early cancer breast (T1 and T2). All patients underwent breast ultrasonography (U/S) with special confirmation on the breast cancer distance from skin surface (radiological distance) in addition to pathological assessment of the distance (pathological distance) after surgical excision and its correlation with radiological distance. Breast conservative surgery (BCS) with axillary clearance was done for 46 patients, while 4 patients underwent modified radical mastectomy (MRM).Results: This study showed that the more proximal the cancer from the skin, the higher the incidence of axillary lymph node (LN) metastasis, and the cut off radiological distance was 2.6cm from the skin while cut off pathological distance was 2.56 cm. It also proved that U/S is an accurate and reliable method in a assessing the breast cancer distance. Conclusion: Our results revealed that cancer breast cancers located closer to the skin surface have a higher incidence of axillary lymph node metastasis.
AMPK deficiency induces DNA methylation and aggravates colorectal tumorigenesis in AOM/DSS mice
Authors: Sun Xiaofei , Tian Qiyu , Xue Yansong , Bibi Shima , Du Min , Zhu Meijun 
Published: May 2020

Abstract
Background: The incidence of colorectal cancer (CRC) is closely related to metabolic diseases. AMP-activated protein kinase (AMPK) is known as a key metabolic regulator. Recently, the regulartory function of AMPK in the perspective of cancer metabolic reprogramming have attracted increasingly attention.Methods: To assess the regulatory role of AMPK in colonic tumorigenesis, we cross-bred AMPKα1-floxed mice with Lgr5Cre mice to specifically knockout AMPK α1 gene in intestinal stem cells, where Lgr5 is expressed, and their derived epithelial cells. The wild-type and AMPK knockout mice were subjected to azoxymethane-induced and dextran sulfate sodium promoted (AOM/DSS) colitis-associated CRC induction. Mechanisms were examined in Caco-2 cells. Stable AMPK knockout cell line was selected using G418.Results: AMPK deficiency caused colonic hyperproliferation and pathological features, which was associated with accelerated CRC development including the augmentation of tumor number, tumor size, and hyperplasia in the AOM/DSS mouse model. The aggravated colorectal tumorigenesis induced by AMPK ablation likely resulted from the alteration of metabolites, as indicated by the reduced production of α-ketoglutarate, an obligate substrate for ten-eleven translocation hydroxylases (TETs) mediated DNA demethylation, in colonic tissues. Consistent with reduced α-ketoglutarate, the content of isocitrate dehydrogenase 1 and the activity of TET1 were markably decreased in AMPK deficient mice, correlated with reduced metastasis inhibitors, Adamts1 (A disintegrin and metalloproteinase with thrombospondin motif 1) and Mal (myelin and lymphocyte), as well as DNA hypermethylation of Mal and Mgmt (O-6-methylguanine-DNA methyltransferase) promotor regions. The mismatch repair (MMR) protein MLH1 or MSH2 levels were downregulated in AMPK deficient Caco-2 cells. AMPK activiation inhibited hypermethylation in promoter regions of Adamts1, Mal and p16ink4a in Caco-2 cells.Conclusions: AMPK deficiency limits α-ketoglutarate concentration and aggregates suppressive epigenetic modifications of antioncogenes in gut epithelial cells, increasing risk of colorectal tumorigenesis. Because AMPK activity is ameable to change, AMPK may serve as a molecular target for colorectal cancer prevention and therapeutic.
Down-regulated CMTM7 promotes metastasis of hepatocellular carcinoma via its family member CMTM3
Authors: Zhu Xiaonian , Bei Chunhua , Li Di , Zhang Huixia , Zeng Wen , Guo Xuefeng , Kong Juan , Fu Yuanyuan , Zhang Shidong , Tan Shengkui 
Published: May 2020

Abstract
Background

Human chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (CMTM) members are found deregulated in various cancers and play a role in cancer development. Recent studies showed that CMTM7 was down-regulated in liver cancer and functioned as a tumor suppressor.
Methods

The expression of CMTM7 in hepatocellular carcinoma (HCC) tissues was measured by bioinformatics analysis, Western blot, and quantitative reverse-transcription polymerase chain reaction (qRT-PCR). CCK8 and cell colony formation assays were conducted to detect the cell proliferation. Cell invasion and migration ability was assessed by wound healing and Transwell assays.
Results

We identified a decreased expression of CMTM7 in HCC tissues was closely related to the metastasis and prognosis of HCC patients. To explore the effect of CMTM7 on the biological function of HCC cells, we over-expressed CMTM7 in two HCC cell lines, SK-Hep-1 and Bel-7402 cells. Cell proliferation was reduced significantly after exogenous expression of CMTM7, detected by both CCK8 and colony formation assay. Moreover, the invasion and migration ability of HCC cells were also inhibited by exogenous expression of CMTM7 in wound healing and Transwell assay. Furthermore, there was a significantly positive correlation between CMTM7 and CMTM3 both in HCC cells and tumor tissues.
Conclusions

Our results suggest that down-regulated CMTM7 promotes metastasis of HCC through inducing the expression of its family member CMTM3.
Severe colon ischemia in patients with severe COVID-2019 infection: a report of three cases
Authors: Almeida Ana , Azcárate Víctor Valentí , Justicia Carlos Sánchez , Regueira Fernando Martínez , Martí-Cruchaga Pablo , Cienfuegos Javier A. , Rotellar Fernando 
Published: May 2020

Abstract
Severe disease caused by the SARS-CoV coronavirus is characterized by patients presenting with respiratory distress associated with a systemic inflammatory response syndrome (cytokine storm). Sixteen to thirty percent of COVID-19 patients also have gastrointestinal symptoms. Here we present three cases of COVID-19 who developed colonic ischemia. Three males aged 76, 68 and 56 with respiratory distress and receiving mechanical ventilation presented episodes of rectal bleeding, abdominal distension and signs of peritoneal irritation. Endoscopy (case 1) and computed tomography angiography revealed colonic ischemia and pneumoperitoneum.One patient (case 2) underwent surgery in which perforation of the gangrenous cecum and colonic ischemia was confirmed.In all three patients D-dimer levels were markedly increased (2170, 2100 and 7360 ng/mL). All three patients died shortly after diagnosis.In severe COVID-19 disease, the pathogenic cause has increasingly become attributed to the development of disseminated intravascular coagulation secondary to the systemic inflammatory response.