bluuebunny/crossref_metadata_2025_split huggingface dataset:
UBQLN4 promotes progression of HCC via activating wnt-β-catenin pathway and is regulated by miR-370
Authors: Yu Yan , Xu Penglin , Cui Guangying , Xu Xiaodong , Li Kongfei , Chen Xiaolong , Bao Jie 
Published: January 2020

Abstract
Background
Ubiquilin-4 (UBQLN4) is a member of the ubiquitin–proteasome system that is usually upregulated in many tumor cells. Its overexpression has been associated with poor disease outcomes in various cancer diseases. However, the underlying mechanism of UBQLN4 in the development of hepatocellular carcinoma (HCC) has not been elucidated.

Methods
Immunochemistry, real-time PCR, and western blotting were used to evaluate the expression levels of UBQLN4 in cancer tissues. Univariate, Cox-regression, and Kaplan–Meier analyses were performed to determine the association between UBQLN4 expression and HCC prognosis. Cell Counting Kit-8 (CCK-8), transwell, EDU and colony formation assays were conducted to evaluate the role of UBQLN4 in HCC cell progression. The gene set enrichment analysis and luciferase reporter experiments were conducted to find the mechanism of UBQLN4 in HCC.

Results
Ubiquilin-4 (UBQLN4) was overexpressed in HCC tissues. Besides, overexpression of UBQLN4 was associated with poor overall survival and disease-free survival rate of HCC patients. The loss-of-function analysis revealed that suppression of UBQLN4 inhibited the proliferation and invasion of HCC cells in vivo and in vitro. The KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that UBQLN4 could regulate activation of the wnt-β-catenin pathway in HCC cells. Furthermore, our results showed that UBQLN4 was downregulated by miR-370, which acted as a tumor suppressor gene in HCC progression.

Conclusion
The results of the present study suggest that the miR-370/UBQLN4 axis may play a critical role in the progression of HCC. These findings may inform future strategies for the development of therapeutic agents against HCC.
Timing of early laparoscopic cholecystectomy for acute calculous cholecystitis revised: Protocol of a systematic review and meta-analysis of results
Authors: Borzellino Giuseppe , Khuri Safi , Pisano Michele , Mansour Subhi , Allievi Niccolò , Ansaloni Luca , Kluger Yoram 
Published: January 2020

AbstractBackgroundEarly laparoscopic cholecystectomy has been adopted as the treatment of choice for acute cholecystitis due to a shorter hospital length of stay and no increased morbidity when compared to delayed cholecystectomy. However, randomised studies and meta-analysis report a wide array of timings of early cholecystectomy, most of them set at 72 h following admission. Setting early cholecystectomy at 72 h or even later may influence analysis due to a shift towards a more balanced comparison. At this time, the rate of resolving acute cholecystitis and the rate of ongoing acute process because of failed conservative treatment could be not so different when compared to those operated with a delayed timing of 6–12 weeks. As a result, randomised comparison with such timing for early cholecystectomy and meta-analysis including such studies may have missed a possible advantage of an early cholecystectomy performed within 24 h of the admission, when conservative treatment failure has less potential effects on morbidity. This review will explore pooled data focused on randomised studies with a set timing of early cholecystectomy as a maximum of 24 h following admission, with the aim of verifying the hypothesis that cholecystectomy within 24 h may report a lower post-operative complication rate compared to a delayed intervention.MethodsA systematic review of the literature will identify randomised clinical studies that compared early and delayed cholecystectomy. Pooled data from studies that settled the early intervention within 24 h from admission will be explored and compared in a sub-group analysis with pooled data of studies that settled early intervention as more than 24 h.DiscussionThis paper will not provide evidence strong enough to change the clinical practice, but in case the hypothesis is verified, it will invite to re-consider the timing of early cholecystectomy and might promote future clinical research focusing on an accurate definition of timing for early cholecystectomy for acute cholecystitis.
A phase I/II study of epertinib plus trastuzumab with or without chemotherapy in patients with HER2-positive metastatic breast cancer
Authors: Macpherson Iain R. , Spiliopoulou Pavlina , Rafii Saeed , Saggese Matilde , Baird Richard D. , Garcia-Corbacho Javier , Italiano Antoine , Bonneterre Jacques , Campone Mario , Cresti Nicola , Posner John , Takeda Yousuke , Arimura Akinori , Spicer James 
Published: December 2019

Abstract
Background
Epertinib (S-222611) is a potent reversible inhibitor of HER2, EGFR and HER4. This trial evaluated the safety, tolerability, pharmacokinetics and antitumour activity of daily oral epertinib combined with trastuzumab (arm A), with trastuzumab plus vinorelbine (arm B) or with trastuzumab plus capecitabine (arm C), in patients with HER2-positive metastatic breast cancer (MBC).

Methods
Eligible patients, with or without brain metastases, had received prior HER2-directed therapy. A dose-escalation phase determined the tolerability of each combination and established a dose for further study. Further, patients were recruited to expansion cohorts in each of the 3 arms to further explore efficacy and safety.

Results
The recommended doses of epertinib were 600 mg, 200 mg and 400 mg in arms A, B and C, respectively. The most frequent grade 3/4 adverse event (AE) was diarrhoea in all arms, which was manageable with medical intervention and dose modification. The objective response rate (complete response [CR] plus partial response [PR]) in heavily pre-treated HER2-positive MBC patients at the recommended doses of epertinib combined with trastuzumab was 67% (N = 9), with trastuzumab plus vinorelbine was 0% (N = 5) and with trastuzumab plus capecitabine was 56% (N = 9). Notably, 4 of 6 patients previously treated with T-DM1 responded in the arm A expansion cohort (epertinib plus trastuzumab). In the arm C expansion cohort (epertinib plus trastuzumab plus capecitabine), 4 of 7 patients responded despite previous exposure to capecitabine. Measurable regression of brain metastases was observed in patients with CNS target lesions treated in both arms A and C.

Conclusion
We observed safety, tolerability and encouraging antitumour activity of epertinib combined with trastuzumab, or with trastuzumab plus capecitabine. This supports further evaluation of these combinations in patients with pre-treated HER2-positive MBC, with or without brain metastases.

Trial registration
EudraCT Number: 2013-003894-87; registered 09-September-2013.
Motor vehicle fatalities during Memorial Day weekends, 1981–2016
Authors: Tang Yuni , Ratnapradipa Kendra L. , Xiang Henry , Zhu Motao 
Published: January 2020

Abstract
Objective
Motor vehicle crashes are a leading cause of injury death in the United States, and Memorial Day weekend is one of six holiday periods with an increased number of motor vehicle fatalities in the United States. However, few motor vehicle fatality comparisons were made between Memorial Day weekend and non-holiday periods. Our aims were to determine which day(s) during the holiday had highest motor vehicle fatality risk compared to non-holiday travel and to identify potential risk factors.

Results
Of 43,457 traffic fatalities studied, 15,292 (35%) occurred during the holiday, with Saturday being deadliest but Monday having highest odds of traffic fatality. Both sexes, all years, age < 65, drivers and passengers, rural and urban, and all regions in the United States were at increased risk during the holiday versus non-holiday periods.
Malfunctioning sufentanil intrathecal pain pump: a case report
Authors: Warner Lindsay , Branstad Anna , Hunter Guevara Lindsay , Matzke Bitterman Laura , Pingree Matthew , Nicholson Wayne , Eldrige Jason 
Published: January 2020

Abstract
Background
Sufentanil is a potent opioid uncommonly used to manage pain and is rarely administered via an intrathecal pain pump system.

Case presentation
This case illustrates the use of intrathecal sufentanil in a 50-year-old Caucasian man for the management of chronic pain; however, the intrathecal drug delivery system experienced a malfunction which led to 1/100th output of the correct dosage. Interesting aspects of this case report include the uncommon choice of sufentanil use for an intrathecal drug delivery system, as well as the unusual pharmacokinetics of this drug. Specifically, this patient did not experience the major withdrawal that would be expected given significant under dosing of opioid, and this may be explained by the lipophilicity and context-sensitive half-times of sufentanil.

Conclusions
Because of the absence of a clinically significant withdrawal in this case report, clinicians must be aware of relevant pharmacokinetic properties and unusual intrathecal drug delivery system technologies that influence a patient’s response when device malfunction occurs.
Early response monitoring of neoadjuvant chemotherapy using [18F]FDG PET can predict the clinical outcome of extremity osteosarcoma
Authors: Lee Inki , Byun Byung Hyun , Lim Ilhan , Kim Byung Il , Choi Chang Woon , Koh Jae-Soo , Song Won Seok , Cho Wan Hyeong , Kong Chang-Bae , Lim Sang Moo 
Published: January 2020

Abstract
Background
To propose a personalized therapeutic approach in osteosarcoma treatment, we assessed whether sequential [18F]FDG PET/CT (PET/CT) could predict the outcome of patients with osteosarcoma of the extremities after one cycle and two cycles of neoadjuvant chemotherapy.

Methods
A total of 73 patients with AJCC stage II extremity osteosarcoma treated with 2 cycles of neoadjuvant chemotherapy, surgery, and adjuvant chemotherapy were retrospectively analyzed in this study. All patients underwent PET/CT before (PET0), after 1 cycle (PET1), and after the completion of neoadjuvant chemotherapy (PET2), respectively. Maximum standardized uptake value (SUVmax) (corrected for body weight) and the % changes of SUVmax were calculated, and histological responses were evaluated after surgery. Receiver-operating characteristic (ROC) curve analyses and the Cox proportional hazards models were used to analyze whether imaging and clinicopathologic parameters could predict event-free survival (EFS).

Results
A total of 36 patients (49.3%) exhibited a poor histologic response and 17 patients (23.3%) showed events (metastasis in 15 and local recurrence in 2). SUVmax on PET2 (SUV2), the percentage change of SUVmax between PET0 and PET1 (Δ%SUV01), and between PET0 and PET2 (Δ%SUV02) most accurately predicted events using the ROC curve analysis. SUV2 (relative risk, 8.86; 95% CI, 2.25–34.93), Δ%SUV01 (relative risk, 5.97; 95% CI, 1.47–24.25), and Δ%SUV02 (relative risk, 6.00; 95% CI, 1.16–30.91) were independent predicting factors for EFS with multivariate analysis. Patients with SUV2 over 5.9 or Δ%SUV01 over − 39.8% or Δ%SUV02 over − 54.1% showed worse EFS rates than others (p < 0.05).

Conclusions
PET evaluation after 1 cycle of presurgical chemotherapy can predict the clinical outcome of extremity osteosarcoma. [18F]FDG PET, which shows a potential role in the early evaluation of the modification of timing of local control, can be a useful modality for early response monitoring of neoadjuvant chemotherapy.
A sliding-neural network control of induction-motor-pump supplied by photovoltaic generator
Authors: Hamdi Hichem , Regaya Chiheb Ben , Zaafouri Abderrahmen 
Published: December 2019

AbstractEnergy production from renewable sources offers an efficient alternative non-polluting and sustainable solution. Among renewable energies, solar energy represents the most important source, the most efficient and the least expensive compared to other renewable sources. Electric power generation systems from the sun’s energy typically characterized by their low efficiency. However, it is known that photovoltaic pumping systems are the most economical solution especially in rural areas. This work deals with the modeling and the vector control of a solar photovoltaic (PV) pumping system. The main objective of this study is to improve optimization techniques that maximize the overall efficiency of the pumping system. In order to optimize their energy efficiency whatever, the weather conditions, we inserted between the inverter and the photovoltaic generator (GPV) a maximum power point adapter known as Maximum Power Point Tracking (MPPT). Among the various MPPT techniques presented in the literature, we adopted the adaptive neuro-fuzzy controller (ANFIS). In addition, the performance of the sliding vector control associated with the neural network was developed and evaluated. Finally, simulation work under Matlab / Simulink was achieved to examine the performance of a photovoltaic conversion chain intended for pumping and to verify the effectiveness of the speed control under various instructions applied to the system. According to the study, we have done on the improvement of sliding mode control with neural network. Note that the sliding-neuron control provides better results compared to other techniques in terms of improved chattering phenomenon and less deviation from its reference.
Endoscope Assisted Microvascular Decompression: Are the Advantages Still Worthy Towards More?
Authors: Farid Ashraf Mohamed , ElKheshin Sherif Elsayed 
Published: January 2020

Abstract
Background
Microvascular decompression is the definitive treatment of various neuralgias affecting cranial nerves. The compression on a cranial nerve could be at the root entry zone, especially the trigeminal nerve. Endoscope-assisted microsurgery may help avoid missing a hidden vascular structure.

Study design
Retrospective clinical case series.

Patient and methods
Twenty-five patients with facial pain and five patients with hemifacial spasm constituted this study. FIESTA MRI was the pre-operative neuroimaging modality. Retrosegmoid craniectomy was done for all patients. Microscope was initially used for exploration and arachnoid dissection around the nerve. The endoscope was applied thereafter for exploration and confirmation of the proper insertion of the Teflon.

Results
Using the endoscope, cerebellar retraction was reduced by 0.5 to 0.8 cm in 90% of patients. Root entry zone and entry of the nerve through the corresponding skull base foramen was clearly visualized by the endoscope. Endoscope enabled a wider area of exploration and panoramic view, which could not be obtained by the microscope. Patients with trigeminal neuralgia had a median pre-operative VAS of 9, while it was only 1 in early post-operative and 0 in 6-month post-operatively. Patients with HFS were completely recovered.

Conclusion
The advantages of microvascular decompression are still worthy. Complications are minimal, and the view is much more panoramic. The different viewing angles and ability to directly reach corners is an absolute endoscopic advantage. Therefore, avoidance of missing vascular structures and incomplete recovery can be assured.
Effect of educational intervention on knowledge and attitude towards research, research ethics, and biobanks among paramedical and administrative teams in the National Liver Institute, Egypt
Authors: EL-Khadry Sally W. , Abdallah Ayat R. , Yousef Marwa F. , M. abdeldayem Hesham , Ezzat Sameera , Dorgham Laila Shehata 
Published: January 2020

Abstract
Background
Medical research has increased greatly in many developing countries during the recent decade, motivated by the need to improve health in these countries. Such research needs to be guided by fundamental ethical principles to ensure the protection of patient’s rights and welfare. Also, biobanks have become increasingly important for the study of health and disease. There is a significant public interest in the outcomes of genetic research, which include diagnostic, therapeutic, and preventive health methods. This study was conducted assess and raise the knowledge and attitude towards several aspects of research, related ethics, and biobank ethical issues for paramedical and administrative teams working at the National Liver Institute (NLI).

Results
The education intervention study was effective in increasing percentage of good knowledge in paramedical and administrative teams (p value < 0.001). Also, the education intervention study was effective in increasing percentage of positive attitude in paramedical and administrative teams (p value < 0.001).

Conclusion
There were good knowledge and attitude about research and related ethics, but poor knowledge and attitude about biobanking. The educational intervention study significantly increased knowledge and attitude about research, related ethics, and biobanks.
Frequency tuning ratio testing of a laser via a hollow photonic crystal fiber resonator
Authors: Lei Ming , Fang Yuan , Yu Huaiyong , Yang Yi , Xiang Qiang , Zhang Lizhe 
Published: January 2020

In order to satisfy the requirements of laser frequency tuning ratio (FTR) measurement, experimental equipment based on a hollow photonic crystal fiber resonator (HPCFR) is proposed in this paper. First, the principle scheme of the equipment consisting of HPCFR is designed, and the resonance curves of the HPCFR are theoretically analyzed, calculated, and simulated; second, the transmissive HPCFR sample is fabricated and the resonance curve is obtained; eventually, the experimental results from the established laser FTR experimental setup demonstrate that the FTRs of a narrow-linewidth fiber laser and semiconductor laser are 17.6 MHz/V and 30.9 MHz/mA, respectively, which are basically in accordance with the factory parameters of the lasers. This work shows that the FTR experimental equipment via HPCFR has the advantages of high precision and good long-term stability.
Arrays of spatially structured non-diffracting optical beams
Authors: Alcídio Varela Mendonça Joel , Zamboni Rached Michel , Ambrosio Leonardo André , Santos Carlos Henrique da Silva 
Published: January 2020

Non-diffracting optical beams and their structured versions have been extensively studied, theoretically and experimentally, over the last two decades, rendering important applications in fields such as imaging, microscopy, remote sensing, optical manipulation, free space optics, etc. In this paper, we theoretically construct arrays of non-coaxial structured non-diffracting beams by using the so-called frozen wave method. We also develop techniques based on polarization allocations and apodizations to mitigate undesirable interferences among neighboring beams. Our results can find interesting applications in all fields that benefit from the use of non-diffracting beams.
KEANEKARAGAMAN DAN KELIMPAHAN ANGGREK EPIFIT DI KAKI GUNUNG LIANGPRAN, KALIMANTAN TIMUR
Authors: Effendi Surianto , Ariyanti Nunik Sri , Chikmawati Tatik 
Published: January 2020

Liangpran Mountain is located within the Heart of Borneo (HoB) Conservation Area which has high biodiversity. The exploration of orchids has been carried out in several locations in the HoB region and recorded a high diversity of wild orchid. This research was conducted to explore and inventory the orchids from the foothill of Mount Liangpran, and record the diversity and abundance of epiphytic orchids in the study sites. This research was carried out by making track of exploration along the Nuyung river estuary to the Tebing Lapah. The richness and abundance data were collected in three plot of 1000 x 20 m. The host tree characteristics and distribution of epiphytic orchid on the host tree were recorded. Fourteen species orchids were collected, and the most dominant are Coelogyne asperata and Agrostophyllum stipulatum with an abundance of 23.53% each. The epiphytic orchids in host trees are spread from the zone II to V based on the Johansson’s sections, in which zone IV is the most diverse. Nine species of the host trees associated with the collected orchids were identified and grouped into five families. The characters of host trees are 7–57 m height,12–112 cm diameters, and 2 types of bark surfaces (rough and intermediate).
KEANEKARAGAMAN DAN SEBARAN EKOLOGIS AMFIBI DI AIR TERJUN BARAMBAISAMARINDA, KALIMANTAN TIMUR
Authors: Jusmaldi Jusmaldi , Setiawan Aditya , Hariani Nova 
Published: January 2020

Diversity and ecological distributions of amphibians in Berambai Waterfall area, East Kalimantan have never been known. The purposes of this study were to determine the diversity of amphibian species in relation to environmental conditions and the vertical-horizontal distribution. A total of 109 amphibians were captured using methods of the transect and the visual encounter survey for 20 days on two type observation habitats. The results of this study found 18 species from six amphibian families. Based on the number of species, the diversity amphibian index and dominance index have a stable of communities, with the high value of Shannon-wiener diversity index (H'), the evenness index (E), and the low value of dominance index (D) were 2.26, 0.78, and 0.16, respectively. Horizontal distribution of amphibians in their habitat showed that aquatic amphibian groups were found to be less than 5 m from the river bank, while terrestrial and semi-aquatic amphibians have a distribution of 5–15 m from the river bank. Vertical distribution of Polypedates leucomystax, Polypedates otilophus and Odorrana hosii in vegetation were found from 1.5–2.5 m above ground level, while Ansonia spinulifer, Chalcorana chalconota, Chalcorana raniceps were at a height of 0.5–<1.5 m from ground level. Diversity of amphibian in Berambai Waterfall area related to habitat conditions, while horizontal and vertical distributions are influenced by species, habits and food resources.
A simulation-based approach to evaluate objective material parameters from concrete rheometer measurements
Authors: Gerland Florian , Wetzel Alexander , Schomberg Thomas , Wünsch Olaf , Middendorf Bernhard 
Published: January 2020

AbstractModern concretes such as ultra-high performance concrete (UHPC) show excellent strength properties combined with favorable flow properties. However, the flow properties depend strongly on process parameters during production (temperature, humidity etc.), but also change sensitively even with slight variations in the mixture. In order to ensure desired processing of the fluidlike material and consistent process quality, the flow properties of the concrete must be evaluated quantitatively and objectively. The usual evaluation of measurements from concrete rheometers, for example of the ball probe system type, does not allow the direct determination of the objective material parameters yield stress and plastic viscosity of the sample. We developed a simulation-based method for the evaluation of rheometric measurements of fine grained high performance concretes like self-compacting concrete (SCC) and UHPC. The method is based on a dimensional analysis for ball measuring systems. Through numerical parameter studies we were able to describe the identified relationship between measuring quantities and material parameters quantitatively for two devices of this type. The evaluation method is based on the Bingham model. With this method it is possible to measure both the yield stress and the plastic viscosity of the fresh sample simultaneously. Device independence of the evaluation process is proven and an application to fiber-reinforced UHPC is presented.