bluuebunny/crossref_metadata_2025_split huggingface dataset:
Demographic and procedural characteristics in the RECording COurses of vasculaR Diseases (RECCORD) registry – the first 1000 patients
Authors: Malyar Nasser , Stausberg Jürgen , Langhoff Ralf , Tatò Federico , Kalka Christoph , Ito Wulf D. , Böhme Jens , Arjumand Jawed , Stegemann Jens , Lawall Holger , Schellong Sebastian , Lichtenberg Michael , Hoffmann Ulrich 
Published: July 2020

Summary: Background: The RECcording COurses of vasculaR Diseases (RECCORD) registry established by the German Society of Angiology – Society for Vascular Medicine aimed to address the lack in contemporary real-world data regarding current practice of medical and interventional care in vascular patients. We herein report the demographic and procedural characteristics of the first 1000 patients undergoing endovascular revascularization (EVR) for symptomatic peripheral artery disease (PAD). Patients and methods: RECCORD is an observational, prospective, multicenter, all-comers registry. Only patients undergoing EVR for symptomatic PAD are included and followed up for at least 1 year. Demographic characteristics, comorbidities, previous peripheral vascular interventions, medication, clinical stage of lower extremity artery disease (Rutherford category), hemodynamic parameters, and procedural data including complications are recorded via an entirely web-based platform. Results: Of the first 1000 patients (mean age 70 ± 10 years, 35% female) with 1096 EVR at 1477 vascular segments of the lower extremities, 25.0% were at the stage of chronic limb threatening ischemia (CLTI) and 75.0% at non-CLTI. The femoropopliteal segment was the dominant target lesion site (61.0%), followed by iliac (26.4%) and below-the-knee EVR (10.3%). Only angioplasty was performed in 130 EVR (11.9%), adjunctive drug coated balloons (DCB) in 498 (45.4%), additional stenting in 633 (57.8%). Debulking devices were used in 106 (9.7%) EVR. Clinical (Rutherford categories) and hemodynamic parameters (ankle-brachial-index) as well as secondary preventive medication were significantly improved post EVR. Periprocedural complications occurred in 63 (5.7%) EVR with pseudoaneurysm as the leading complication type in 26 (2.4%) EVR. Conclusions: The baseline data of the first 1000 patients from the RECCORD registry representing the real-world setting illustrate that the majority of EVR are performed in patients with claudication. Adjunctive use of DCB and stenting are the dominant types of EVR, while periprocedural complications are at an acceptable low rate.
Spatial Characteristics on the Occurrence of the Nighttime Midlatitude Medium‐Scale Traveling Ionospheric Disturbance at Topside Ionosphere Revealed by the Swarm Satellite
Authors: Wan Xin , Xiong Chao , Wang Hui , Zhang Kedeng , Yin Fan 
Published: July 2020

AbstractUsing 6 years collecting of electron density (Ne) data from the Swarm A satellite, this study portrays comprehensive maps of the occurrence of medium‐scale traveling ionospheric disturbance (MSTID) at middle latitudes, which is characterized by the in situ absolute Ne fluctuations above a certain threshold. Two interesting spatial preferences on the occurrence of MSTID as well as their dependence on local time and solar cycle are captured, that is, (1) regional preference near Weddell Sea Anomaly (WSA) to its west during December solstice, MSTID in this region appears even at very low latitudes (at least to 10°N in magnetic latitude) and (2) preference at middle latitudes with a wide zonal extension, which is similar to the recently disclosed nighttime midlatitude plasma band‐like enhancement (NMPBE) of the background plasma density (Zhong et al., 2019, https://doi.org/10.1029/2018JA026059). The first preference of MSTID occurring near WSA region can be understood as the effects of horizontal neutral wind contribution to the Perkins instability, with the principal requirement of sporadic E occurrence via ionospheric E‐F region coupling. The fundamental mechanism of the second preference of MSTID occurring includes the interhemispheric coupling that the MSTID can also develop in the winter hemisphere where the local growth rate of Perkins instability is low. Besides, the presence of NMPBE during low solar activity years sets up a favorable environment for the appearance of MSTID with considerable fluctuation amplitude.
Energetics and Alfvénic Coupling of a Poleward Boundary Intensification: A Polar Case Study
Authors: Keiling Andreas , Wygant John , Fillingim Matthew , Trattner Karlheinz J. 
Published: July 2020

AbstractThe poleward boundary intensification (PBI) is a common appearance at the poleward boundary of the auroral bulge and auroral oval. The PBI presented here occurred during the expansion phase of a small substorm with an auroral surge power of 6.5 GW. The auroral power of the PBI (1.1 GW) was ~17% of this value. The largest powers above the nominal auroral acceleration region at 5 RE geocentric were carried by Alfvén waves (1.7 GW) and Alfvénic electrons (0.7 GW), sufficient to account for the conjugate PBI auroral power. In contrast, the conjugate quasistatic, field‐aligned current power (<0.3 GW) was not sufficient. Observed correlation between quasiperiodic Alfvénic pulses and auroral modulations strengthens our conclusion that the electromagnetic magnetosphere‐ionosphere coupling of the PBI was dominantly Alfvénic, as opposed to electrostatic, thus causing Alfvénic aurora.
Freshening of Antarctic Bottom Water Off Cape Darnley, East Antarctica
Authors: Aoki S. , Katsumata K. , Hamaguchi M. , Noda A. , Kitade Y. , Shimada K. , Hirano D. , Simizu D. , Aoyama Y. , Doi K. , Nogi Y. 
Published: July 2020

AbstractRecently, a source of Antarctic Bottom Water (AABW) was identified off Cape Darnley at the eastern end of the Weddell‐Enderby Basin. However, the behavior and long‐term variability of Cape Darnley Bottom Water (CDBW) are not clearly understood. Hydrographic observations from 1974 to 2016 were compared, and a decade‐long bottom temperature record was analyzed to clarify multidecadal changes in the CDBW in this region and its downstream influences. In the Cooperation Sea, CDBW spread northwestward with its deepest part reaching to approximately 4,900 dbar. CDBW freshening of 0.001–0.003 decade−1 was revealed. In the Cosmonaut Sea, long‐term AABW warming of approximately 0.01–0.03°C decade−1 was prominent in the deep basin, while freshening was detected on the upper continental slope. Spatial patterns suggest that an interbasin deep transport of excess freshwater is carried by CDBW and fed into the Weddell Gyre, which might act as an abyssal freshwater buffer.
Photoperiod Explains the Asynchronization Between Vegetation Carbon Phenology and Vegetation Greenness Phenology
Authors: Kong Dongdong , Zhang Yongqiang , Wang Dagang , Chen Jianyao , Gu Xihui 
Published: July 2020

AbstractVegetation carbon phenology is a key indicator of vegetation actual photosynthesis activity and regulates terrestrial ecosystem carbon balance. A comprehensive understanding of the structural difference of carbon phenology and greenness phenology is still lacking. This study evaluated the structural difference of vegetation greenness phenology and carbon phenology on 95 eddy covariance flux sites (637 site‐years). Vegetation greenness phenology is extracted from remote sensing vegetation indices (i.e., enhanced vegetation index [EVI] and normalized difference vegetation index [NDVI]) of Moderate Resolution Imaging Spectroradiometer satellites; carbon phenology is extracted from daily gross primary production estimates at flux sites. We found that there exists remarkable asynchronization between vegetation greenness phenology and carbon phenology on the 95 flux sites. Generally, the asynchronization is more prevalent in the withering season, with the bias about 18.7 ± 13.0 days for EVI and about 29.8 ± 14.4 days for NDVI. In comparison, in the green‐up season, the bias is only about −1.6 ± 12.9 days for EVI and −6.0 ± 13.8 days for NDVI. We found that photoperiod is the dominant factor controlling the asynchronization and the decrease of photosynthetic capacity in the withering season, other than photosynthetically active radiation or temperature. Our study also reveals that EVI × PC and NDVI × PC are good proxies of vegetation carbon phenology, where PC is the photoperiod constraint and is quantified as
. Using PC in the withering season reduces the bias of EVI from 18.7 ± 13.0 days to −2.4 ± 11.2 days and the bias of NDVI from 29.8 ± 14.4 days to −1.1 ± 12.7 days.
The Cenozoic Evolution of Crustal Shortening and Left‐Lateral Shear in the Central East Kunlun Shan: Implications for the Uplift History of the Tibetan Plateau
Authors: Staisch Lydia M. , Niemi Nathan A. , Clark Marin K. , Chang Hong 
Published: July 2020

AbstractThe timing of crustal shortening and strike‐slip faulting along the East Kunlun Shan provides insight into the history of surface uplift and may constrain the time at which the Tibetan Plateau reached high elevations. We investigate a series of extensional basins and restraining bends along the Xidatan strand of the Kunlun strike‐slip fault, which provide an ideal setting to unravel the tectonic history of the northern plateau margin. We present new apatite (U‐Th)/He, apatite fission track, and zircon (U‐Th)/He ages and QTQt thermal modeling, 40Ar/39Ar fault gouge dating, and structural mapping from the central East Kunlun Shan. Our data suggest that the East Kunlun Shan experienced slow to negligible exhumation until late Cretaceous time, followed by an increase in rate by 65–50 Ma. Along with a ~47 Ma fault gouge age, we posit that the Paleocene–early Eocene was a time of crustal shortening along the northern plateau. Rapid exhumation along transpressional portions of the Xidatan fault initiated by 23–20 Ma, which we interpret as the local onset of strike‐slip faulting. An early Miocene transition from north‐south crustal shortening to left‐lateral shear along the East Kunlun Shan, the onset of normal and strike‐slip faulting in central and southern Tibet by 18 Ma, and lower crustal flow in eastern Tibet by 13 Ma suggest the establishment of orogen‐wide east‐west oriented extension and extrusion by the middle Miocene. The plateau‐wide shift in stress accommodation implies that high gravitational potential energy, and likely high elevation, was attained by the middle Miocene.
Dissociation of functional and structural plasticity of dendritic spines during NMDAR and mGluR-dependent long-term synaptic depression in wild-type and fragile X model mice
Authors: Thomazeau Aurore , Bosch Miquel , Essayan-Perez Sofia , Barnes Stephanie A. , De Jesus-Cortes Hector , Bear Mark F. 
Published: July 2020

AbstractMany neurodevelopmental disorders are characterized by impaired functional synaptic plasticity and abnormal dendritic spine morphology, but little is known about how these are related. Previous work in the Fmr1-/y mouse model of fragile X (FX) suggests that increased constitutive dendritic protein synthesis yields exaggerated mGluR5-dependent long-term synaptic depression (LTD) in area CA1 of the hippocampus, but an effect on spine structural plasticity remains to be determined. In the current study, we used simultaneous electrophysiology and time-lapse two photon imaging to examine how spines change their structure during LTD induced by activation of mGluRs or NMDA receptors (NMDARs), and how this plasticity is altered in Fmr1-/y mice. We were surprised to find that mGluR activation causes LTD and AMPA receptor internalization, but no spine shrinkage in either wildtype or Fmr1-/y mice. In contrast, NMDAR activation caused spine shrinkage as well as LTD in both genotypes. Spine shrinkage was initiated by non-ionotropic (metabotropic) signaling through NMDARs, and in wild-type mice this structural plasticity required activation of mTORC1 and new protein synthesis. In striking contrast, NMDA-induced spine plasticity in Fmr1-/y mice was no longer dependent on acute activation of mTORC1 or de novo protein synthesis. These findings reveal that the structural consequences of mGluR and metabotropic NMDAR activation differ, and that a brake on spine structural plasticity, normally provided by mTORC1 regulation of protein synthesis, is absent in FX. Increased constitutive protein synthesis in FX appears to modify functional and structural plasticity induced through different glutamate receptors.
Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction
Authors: Song Fangda , Chan Ga Ming Angus , Wei Yingying 
Published: July 2020

AbstractDespite their widespread applications, single-cell RNA-sequencing (scRNA-seq) experiments are still plagued by batch effects and dropout events. Although the completely randomized experimental design has frequently been advocated to control for batch effects, it is rarely implemented in real applications due to time and budget constraints. Here, we mathematically prove that under two more flexible and realistic experimental designs—the reference panel and the chain-type designs—true biological variability can also be separated from batch effects. We develop Batch effects correction with Unknown Subtypes for scRNA-seq data (BUSseq), which is an interpretable Bayesian hierarchical model that closely follows the data-generating mechanism of scRNA-seq experiments. BUSseq can simultaneously correct batch effects, cluster cell types, impute missing data caused by dropout events, and detect differentially expressed genes without requiring a preliminary normalization step. We demonstrate that BUSseq outperforms existing methods with simulated and real data.
Caspase dependent apoptosis is required for anterior regeneration in Aeolosoma viride and its related gene expressions are regulated by the Wnt signaling pathway
Authors: Fok Sheridan Ke-Wing , Chen Chiao-Ping , Tseng Tzu-Lun , Chiang Yi-Hua , Chen Jiun-Hong 
Published: July 2020

AbstractAlthough apoptosis has been widely observed during the regenerative process, the mechanisms by which it is regulated and its roles in regeneration remained unclear. In this study, we introduced Aeolosoma viride, a fresh water annelid with an extraordinary regenerative ability as our model organism to study the functions and regulations of apoptotic caspases. Here we showed that major events of apoptosis were detected near the wounded area and showed spatial correlation with the expression patterns of caspase gene namely Avi-caspase X and two apoptosis regulators namely Avi-Bax and Avi-Bcl-xL. Next, we investigated how Avi-caspase X gene expression and apoptosis influence regeneration following head amputation. RNA interference of Avi-caspase X reduced the amounts of apoptotic cells, as well as the percentage of successful regeneration, suggesting a critical role for apoptosis in anterior regeneration of A. viride. In addition, we also discovered that the expression of apoptotic caspases was regulated by the canonical Wnt signaling pathway. Together, our study showed that caspase dependent apoptosis was critical to the anterior regeneration of A. viride, and could be regulated by the canonical Wnt signaling pathway.
The Geriatric Nutritional Risk Index predicts postoperative complications and prognosis in elderly patients with colorectal cancer after curative surgery
Authors: Sasaki Masaru , Miyoshi Norikatsu , Fujino Shiki , Ogino Takayuki , Takahashi Hidekazu , Uemura Mamoru , Matsuda Chu , Yamamoto Hirofumi , Mizushima Tsunekazu , Mori Masaki , Doki Yuichiro 
Published: July 2020

AbstractMalnutrition has been considered to be associated with the prognosis of cancer. The Geriatric Nutritional Risk Index (GNRI), based on serum albumin levels, present body weight, and ideal body weight, is a simple screening tool to predict the risk of nutrition-related morbidity and mortality in elderly patients. We aimed to evaluate whether preoperative GNRI was associated with postoperative complications and prognosis in elderly patients with colorectal cancer (CRC). We retrospectively enrolled 313 CRC patients aged ≥65 years after curative surgery and classified them into an all-risk GNRI (≤98) group and a no-risk GNRI (>98) group. Kaplan-Meier analysis showed overall survival was significantly worse in the all-risk GNRI group than in the no-risk GNRI group (P = 0.009). Multivariable analyses showed low GNRI (≤98) was an independent risk factor for postoperative complications (P = 0.048) and overall survival (P = 0.001) in the patients. Among the complications, the incidence of surgical site infection, in particular, was significantly higher in the all-risk GNRI group (P = 0.008). In conclusion, low preoperative GNRI (≤98) was associated with increased postoperative complications and poor prognosis. Preoperative GNRI can be used as an identifier for potential high-risk group of morbidity and mortality in elderly CRC patients.
Targeted next-generation sequencing of sputum for diagnosis of drug-resistant TB: results of a national survey in Democratic Republic of the Congo
Authors: Kayomo Michel Kaswa , Mbula Vital Nkake , Aloni Muriel , André Emmanuel , Rigouts Leen , Boutachkourt Fairouz , de Jong Bouke C. , Nkiere Nicolas M. , Dean Anna S. 
Published: July 2020

AbstractThe surveillance of drug resistance among tuberculosis (TB) patients is central to preventing the spread of antimicrobial resistance. The Democratic Republic of the Congo (DR Congo) is classified by the World Health Organization (WHO) as a country with a high burden of TB and multidrug-resistant TB (MDR-TB), but there are no nationally representative data on drug resistance. In 2016–2017, a national survey of TB patients was conducted in 108 microscopy centres across all 11 provinces of the country using innovative molecular approaches. Sputum samples were collected from 1,545 new and 163 previously treated patients. These were tested by the Xpert MTB/RIF assay, followed by targeted next-generation sequencing performed directly on sputum. The prevalence of rifampicin resistance was low, at 1.8% (95% CI: 1.0–3.2) among new and 17.3% (95% CI: 11.9–24.4) among previously treated patients. Resistance to pyrazinamide, fluoroquinolones and second-line injectables was also low. The prevalence of resistance to isoniazid among rifampicin-susceptible patients was higher, at 6.6% (95% CI: 4.4–9.8) among new and 8.7% (95% : 3.2–21.2) among previously treated patients. Diagnosing and treating isoniazid-resistant patients remains a challenge, given that many will be missed by the current national diagnostic algorithm that is driven by detecting rifampicin resistance by Xpert MTB/RIF. This is the first nationwide survey incorporating targeted sequencing directly on sputum. It serves as a proof-of-concept for other settings that do yet have rapid specimen transport networks or capacity to conduct culture.
The biological age of the heart is consistently younger than chronological age
Authors: Pavanello Sofia , Campisi Manuela , Fabozzo Assunta , Cibin Giorgia , Tarzia Vincenzo , Toscano Giuseppe , Gerosa Gino 
Published: July 2020

AbstractChronological age represents the main factor in donor selection criteria for organ transplantation, however aging is very heterogeneous. Defining the biological aging of individual organs may contribute to supporting this process. In this study we examined the biological age of the heart [right (RA)/left atrium (LA)] and peripheral blood leucocytes in the same subject, and compared these to assess whether blood mirrors cardiac biological aging. Biological aging was studied in 35 donors (0.4–72 years) by exploring mitotic and non-mitotic pathways, using telomere length (TL) and age-dependent methylation changes in certain CpG loci (DNAmAge). Heart non-mitotic DNAmAge was strongly younger than that of both blood (− 10 years, p < 0.0001) and chronological age (− 12 years, p < 0.0001). Instead, heart and blood mitotic age (TL) were similar, and there was no difference in DNAmAge and TL between RA and LA. DNAmAge negatively correlated with TL in heart and blood (p ≤ 0.01). Finally, blood and heart TL (p < 0.01) and DNAmAge (p < 0.0001) were correlated. Therefore, blood can be a proxy indicator of heart biological age. While future investigation on post-transplant graft performance in relation to biological aging is still needed, our study could contribute to opening up novel basic and clinical research platforms in the field of organ transplantation.
Human papillomavirus vaccine-associated premature ovarian insufficiency and related adverse events: data mining of Vaccine Adverse Event Reporting System
Authors: Gong Li , Ji Huan-huan , Tang Xue-wen , Pan Ling-yun , Chen Xiao , Jia Yun-tao 
Published: July 2020

AbstractWe detected disproportionate reports of premature ovarian insufficiency (POI) and related events, including amenorrhea, menstruation irregular, FSH increased, and premature menopause, following human papillomavirus (HPV) vaccine from FDA Vaccine Adverse Event Reporting System (VAERS). The signal was detected by the methods of Bayesian Confidence Propagation Neural Network (BCPNN) and Multi-item Gamma Poisson Shrinker (MGPS). When both methods detected a positive result, a signal was generated. Besides, time-scan map is drawn based on the IC value and 95%CI of BCPNN, if the IC curve showed a steady upward trend and the 95%CI narrowed, the signal was stable and strong association.The results showed that there were not POI reports of HPV vaccine, but VAERS received a total of 2, 389, 27 POI related events for HPV2, HPV4, HPV9 respectively from the year of marketed to 2018. No signal was detected for HPV2. HPV4-POI ralated events were all detected as signals by two methods. There was only one signal of menstruation irregular for HPV9. Time scan of HPV4-POI ralated events showed those signals were stability and strong association, but not for HPV9. Our results only represent statistical association between HPV vaccine and POI related events, causal relationship needs further investigation.
Diagnostic performance of contrast-enhanced ultrasound for acute pyelonephritis in children
Authors: Jung Hyun Joo , Choi Moon Hyung , Pai Ki Soo , Kim Hyun Gi 
Published: July 2020

AbstractThe objective of our study was to evaluate the performance of renal contrast-enhanced ultrasound (CEUS) against the 99m-labeled dimercaptosuccinic acid (DMSA) scan and computed tomography (CT) in children for the diagnosis of acute pyelonephritis. We included children who underwent both renal CEUS and the DMSA scan or CT. A total of 33 children (21 males and 12 females, mean age 26 ± 36 months) were included. Using the DMSA scan as the reference standard, the sensitivity, specificity, positive predictive value, and negative predictive value of CEUS was 86.8%, 71.4%, 80.5%, and 80.0%, respectively. When CT was used as the reference standard, the sensitivity, specificity, positive predictive value, and negative predictive value of CEUS was 87.5%, 80.0%, 87.5%, and 80.0%, respectively. The diagnostic accuracy of CEUS for the diagnosis of acute pyelonephritis was 80.3% and 84.6% compared to the DMSA scan and CT, respectively. Inter-observer (kappa = 0.54) and intra-observer agreement (kappa = 0.59) for renal CEUS was moderate. In conclusion, CEUS had good diagnostic accuracy for diagnosing acute pyelonephritis with moderate inter- and intra-observer agreement. As CEUS does not require radiation or sedation, it could play an important role in the future when diagnosing acute pyelonephritis in children.