bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensory attributes of coffee beverages and their relation to price and package information: A case study of Colombian customers’ preferences
Authors: Barahona Igor , Sanmiguel Jaimes Edis Mauricio , Yang Jian‐Bo 
Published: January 2020

AbstractSensory analysis is a powerful tool for creating profiles of food and beverages based on information perceived by the human senses. This paper investigates 18 of the most popular Colombian coffees. Individuals from nine different cities assessed products in two different ways: degree of presence (absence) of sensory properties and degree of acceptance (liking). The results focused on identifying variations in sensory evaluations due to the city, as well as classification of the products according to their degree of acceptance or rejection, and investigating associations between sensory attributes, price, and label–package information. A correspondence analysis allowed us to investigate the variation introduced by the factor city. The most preferred/rejected products were identified through preference mapping. The level of intensity of the smelling sensory attribute positively affects the price and the information presented at the product´s label–package. However, tasting attributes negatively affects price and perceptions of the product´s label–package information. We conclude that smelling sensory attributes has greater impact on purchase intentions than tasting attributes. Decision‐makers should manage scent, price, and label–package characteristics wisely because they are part of the first experience of the customer.
DNA Encoded Libraries: A Visitor's Guide
Authors: Flood Dillon T. , Kingston Cian , Vantourout Julien C. , Dawson Philip E. , Baran Phil S. 
Published: January 2020

AbstractIn 1992, Brenner and Lerner hypothesized that individual chemical transformations could be encoded in DNA, allowing the rapid synthesis and screening of large collections of small molecules. Since their report, huge investments into the development of the DNA encoded library (DEL) technology have enabled the acceleration of the drug discovery process especially early phase discovery undertakings such as target validation and hit identification. As DEL lies at the nexus between chemistry and biology, there is an increasing need to expand the toolboxes of both organic transformations and biological methods. However, the myriad of techniques and reactions already reported can be difficult to digest for practitioners whose expertise resides outside the realm of DEL. This review therefore focuses on a stepwise presentation of DEL from the basic concepts to newest developments. The presentation includes the history, fundamentals, and successes of DEL, different methods for DEL synthesis and affinity selection, the conventional transformations, and finally the latest developments from a synthetic organic perspective.
Computational characterization of the porous‐fibrous behavior of the soft tissues in the temporomandibular joint
Authors: Ortún‐Terrazas Javier , Cegoñino José , Pérez del Palomar Amaya 
Published: January 2020

AbstractThe prevalence and severity of temporomandibular joint (TMJ) disorders have led to growing research interest in the development of new biomaterials and medical devices for TMJ implant designs. In computational designs, however, the time and stretch direction dependences of the TMJ soft tissues behavior are not considered and they are frequently based on measurements taken from non‐human species or from joints that differ markedly from the human TMJ. The aim of this study was to accurately characterize the porous‐fibrous properties of the TMJ soft tissues by simulating previously published experimental tests, to assist professionals in the design of new TMJ implants. To that end, material parameters were determined assuming a uniform fiber orientation throughout the entire sample. This assumption was then tested by comparing these results with those of considering multiple regions and distinct fiber orientations in each sample. Our findings validated the use of a transversely isotropic hyperelastic material model to characterize the direction dependent behavior of TMJ soft tissues and its combination with porous hyperfoam material models to mimic the compressive response of the TMJ disc. In conclusion, constitutive model proposed accurately reproduce the mechanical response of the TMJ soft tissues at different strain rates and stretch directions.
A ratiometric fluorescent probe with high sensitivity and selectivity for phosgene sensing in solution and gas
Authors: Zhou Wu , Chen Qian , Wu Aibin , Zhang Ying , Yu Weichu 
Published: January 2020

AbstractPhosgene has attracted wide attention because of its important applications and value in modern industry, agriculture, and other fields, though it easily leaks and is difficult to detect. In this work, we designed and synthesized a naphthalimide‐based fluorescent probe, which is easy to prepare, stable, and able to discriminate between phosgene, acetyl chloride, oxalyl chloride, thionyl chloride, phosphorus oxychloride, and tosyl chloride. Our results indicate that the probe can react with phosgene selectively and sensitively, showing remarkable ratiometric fluorescence changes. Furthermore, the probe can be made into test strips, which can determine phosgene in air effectively. The present work provides a novel class of naphthalimide‐based derivatives with potential application in phosgene sensing in real time simply and safely with further optimization.
Critical roles of long noncoding RNAs in breast cancer
Authors: Abolghasemi Maryam , Tehrani Sadra S. , Yousefi Tooba , Karimian Ansar , Mahmoodpoor Ata , Ghamari Aliakbar , Jadidi‐Niaragh Farhad , Yousefi Mehdi , Kafil Hossein S. , Bastami Milad , Edalati Mahdi , Eyvazi Shirin , Naghizadeh Mohsen , Targhazeh Niloufar , Mihanfar Ainaz , Yousefi Bahman , Safa Amin , Majidinia Maryam , Rameshknia Vahid 
Published: January 2020

AbstractBreast cancer is a major clinical challenge that affects a wide range of the female population and heavily burdens the health system. In the past few decades, attempts have been made to understand the etiology of breast cancer, possible environmental risk factors, and the genetic predispositions, pathogenesis, and molecular aberrations involved in the process. Studies have shown that breast cancer is a heterogeneous entity; each subtype has its specific set of aberrations in different cell signaling pathways, such as Notch, Wnt/β‐catenin, transforming growth factor‐β, and mitogen‐activated protein kinase pathways. One novel group of molecules that have been shown to be inducted in the regulation of multiple cell signaling pathways is the long noncoding RNAs (lncRNAs). These molecules have important implications in the regulation of multiple signaling pathways by interacting with various genes, affecting the transcription process, and finally, playing roles in posttranslational control of these genes. There is growing evidence that lncRNAs are involved in the process of breast cancer formation by effecting the aforementioned signaling pathways, and that this involvement can have significant diagnostic and prognostic values in clinical contexts. The present review aims to elicit the significance of lncRNAs in the regulation of cell signaling pathways, and the resulting changes in cell survival, proliferation, and invasion, which are the hallmarks of breast cancer.
Highly selective carbon nanotube‐coupled graphene oxide‐incorporated polydimethylsiloxane membrane for pervaporative membrane bioreactor ethanol production
Authors: Shafiei Amrei Shiva , Asghari Morteza , Esfahanian Mehri , Zahraei Zohreh 
Published: January 2020

AbstractBACKGROUNDIn this work, a pervaporation membrane bioreactor (PVMBR) with the novel ethanol permselective membrane was used to achieve ethanol production with high yield. For this purpose, ethanol produced by Saccharomyces cerevisiae from glucose and pervaporation was performed via dense hydrophobic polydimethylsiloxane (PDMS) having carbon nanotube‐graphene oxide (CNT‐GO) mixed matrix membrane. Different percentages of CNT‐GO (0, 0.25, 0.5, 0.75, and 1) were loaded in polymer matrix. Synthesized membranes were analyzed using FESEM, AFM, FTIR and XRD.RESULTSThe bonding of groups in CNT‐GO, that was synthesized in this work, and membranes was characterized and confirmed using FTIR test. Considering the existence of 0.75 wt% of CNT‐GO in PDMS showed the highest ethanol selectivity of 22.5 in synthetic ethanol‐water solution, this incorporation was selected to use in PVMBR. The high ethanol selectivity was proved using XRD patterns. Also, FESEM indicated the optimum dispersion of nanoparticles at 0.75% of loading and AFM proofed the optimum surface roughness at this percentage.CONCLUSIONSThe results showed that maximum ethanol production in PVMBR was achieved after 44 h with productivity of 0.5g.l−1.h−1 in feed side and 9.89 g.l−1.h−1 in permeate side. In fact, PVMBR enhanced the ethanol concentration up to 26 times. © 2020 Society of Chemical Industry
A comparison between Suzuki cross‐coupling reaction and direct arylation in the synthesis of new antibacterial imidazo‐pyrazines/pyridazines
Authors: Salek Soltani Siavash , F. Farnia S. Morteza , Foroumadi Alireza 
Published: January 2020

AbstractTwo efficient synthetic routes to preparation of 2,3‐diarylimidazo[1,2‐a]pyrazines and 2,3‐diarylimidazo[1,2‐b]pyridazines are described. The procedures involve a Suzuki cross‐coupling reaction and a palladium‐catalyzed direct arylation at position 3 and finally a comparative study is achieved. The antibacterial activities of the synthesized compounds in vitro were measured and the results showed that most products presented moderate to good antibacterial activities. Compounds 3a, 3c, 3j, 3k, 3l, and 3n revealed obvious potency against Staphylococcus aureus, Bacillus subtilis, and Escherichia coli with minimum inhibitory concentration values of 32 μg/mL, which were better compared with the others.
Oxygen and nitrite reduction by heme‐deficient sulphite oxidase in a patient with mild sulphite oxidase deficiency
Authors: Bender Daniel , Kaczmarek Alexander T. , Kuester Sabina , Burlina Alberto B. , Schwarz Guenter 
Published: January 2020

AbstractIsolated sulphite oxidase deficiency (iSOD) is an autosomal recessive inborn error in metabolism characterised by accumulation of sulphite, which leads to death in early infancy. Sulphite oxidase (SO) is encoded by the SUOX gene and forms a heme‐ and molybdenum‐cofactor‐dependent enzyme localised in the intermembrane space of mitochondria. Within SO, both cofactors are embedded in two separated domains, which are linked via a flexible 11 residue tether. The two‐electron oxidation of sulphite to sulphate occurs at the molybdenum active site. From there, electrons are transferred via two intramolecular electron transfer steps (IETs) via the heme cofactor and to the physiologic electron acceptor cytochrome c. Previously, we reported nitrite and oxygen to serve as alternative electron acceptors at the Moco active site, thereby overcoming IET within SO. Here, we present evidence for these reactions to occur in an iSOD patient with an unusual mild disease representation. In the patient, a homozygous c.427C>A mutation within the SUOX gene leads to replacement of the highly conserved His143 to Asn. The affected His143 is one of two heme‐iron‐coordinating residues within SO. We demonstrate, that the H143N SO variant fails to bind heme in vivo leading to the elimination of SO‐dependent cytochrome c reduction in mitochondria. We show, that sulphite oxidation at the Moco domain is unaffected in His143Asn SO variant and demonstrate that nitrite and oxygen are able to serve as electron acceptors for sulphite‐derived electrons in cellulo. As result, the patient H143N SO variant retains residual sulphite oxidising activity thus ameliorating iSOD progression.
Obesity retunes turnover kinetics of tissue-resident macrophages in fat
Authors: Chen Qi , Ruedl Christiane 
Published: January 2020

Abstract
Adipose tissue-resident F4/80hi macrophages (ATMs) are the main leukocyte population found in the visceral adipose tissue (VAT). These macrophages comprise several phenotypically distinct subpopulations that rapidly shift in abundance during obesity-induced tissue remodeling. Here we used a fate-mapping approach in mouse models to determine the developmental origins and the differential turnover kinetics of ATMs in lean and obese adipose tissue. We found that in lean, murine VAT the majority of ATMs express T cell immunoglobulin and mucin domain containing 4 receptor (Tim-4), lack the expression of CCR2 and can be further subdivided based on their expression of MHC class II and CD11c. We showed that both embryonic-derived Tim-4+MHCIIlow and Tim-4+MHCII+ ATM subsets are long-lived and only slowly replenished by monocytes over time. Only a minor Tim-4−MHCII+CD11c+ ATM fraction expresses CCR2 and is short-lived. In response to high-fat induced VAT remodeling, the majority of Tim-4+MHCIIlow ATMs maintain their fetal identity as they are moderately displaced by monocytes. Conversely, Tim-4+MHCII+ ATMs are quickly replaced in a CCR2-dependent manner by bone marrow-derived Tim-4−MHCII+ ATMs that have significantly higher turnover rates than those in lean mice. In addition, during high-fat diet, the subpopulation of CD11c+ macrophages invade the VAT with the fastest turnover kinetics of all three ATM subpopulations. Our results suggest that ATM subpopulation frequency is controlled by the VAT microenvironment and that obesity-induced tissue remodeling renders some of the ATM niches accessible and available for rapid monocyte replenishment. Specialized monocyte-derived macrophages, which are rapidly recruited may be contributing to control the excess of adipocyte-released lipids produced during obesity.
Detection of saliva morphine using surface‐enhanced Raman spectroscopy combined with immunochromatographic assay
Authors: Li Wei , Li Xiaozhou , Yang Tianyue , Guo Xingjia , Song Youtao 
Published: January 2020

AbstractAn immunochromatographic assay (ICA) based on surface‐enhanced Raman scattering (SERS) method was developed for the detection of morphine in this study. Indirect competitive immunoadsorption was adopted in the ICA due to the small molecular weight of morphine. Antibody modified gold nanoparticles in the ICA were labeled with Raman reporters and were used as the SERS enhancement substrate. By measuring the feature peaks of Raman reporter MBA at 1,078 cm−1, a quantitative estimation of morphine can be obtained. The limit of detection of this ICA‐SERS method was 2.4 × 10−4 ng/ml for morphine, which was four orders of magnitude lower than the visual method. This study demonstrated that the proposed ICA‐SERS method is a good candidate for the rapid and accurate detection of illicit drugs in saliva. All the analyses in this paper were carried out in a Microsoft (MS) Excel platform using embedded R functions through Excel BERT add‐in.
Cardiac protection of functional chicken‐liver hydrolysates on the high‐fat diet induced cardio‐renal damages via sustaining autophagy homeostasis
Authors: Wu Yi‐Hsieng Samuel , Lin Yi‐Ling , Huang Chien , Chiu Chih‐Hsien , Nakthong Sasitorn , Chen Yi‐Chen 
Published: January 2020

AbstractBACKGROUNDCardio‐renal syndrome (CRS) is an integrative problem related to chronic malnutrition, obesity, etc. Amino acids and peptides are regarded as protective and essential for tissues. Pepsin‐digested chicken liver hydrolysates (CLHs), which are made from the byproducts of the poultry industry, are amino‐acid based and of animal origin, and may be protective against the myocardial and renal damage induced by a high‐fat diet (HFD).RESULTSOur results showed that CLHs contain large quantities of anserine, taurine, and branched‐chain amino acids (BCAAs), and supplementing the diet with CLHs reduced (P < 0.05) weight gain, liver weight, peri‐renal fat mass / adipocyte‐area sizes, serum total cholesterol (TC), aspartate aminotransferase (AST), and low‐density lipoprotein cholesterol (LDLC) levels in HFD‐fed mice but increased (P < 0.05) serum high‐density lipoprotein cholesterol (HDLC) levels. By histological analyses, CLHs alleviated (P < 0.05) renal lipid deposition and fibrosis, as well as cardiac fibrosis and inflammation of HFD‐fed mice. Meanwhile, increased (P < 0.05) inflammatory and fibrotic cytokines levels in the myocardia of the HFD‐fed mice were downregulated (P < 0.05) by CLH supplementation. Regarding autophagy‐related protein levels, protective effects of CLHs on the myocardia against HFD feeding may result from the early blockade of the autophagy pathway to prevent autophagosome accumulation.CONCLUSIONFunctional CLHs could be a novel food ingredient as a cardio‐renal protective agent against a high‐fat dietary habit in a niche market. © 2020 Society of Chemical Industry
A Systematic Review of DNA Methylation and Gene Expression Studies in Posttraumatic Stress Disorder, Posttraumatic Growth, and Resilience
Authors: Mehta Divya , Miller Olivia , Bruenig Dagmar , David Georgina , Shakespeare‐Finch Jane 
Published: January 2020

AbstractMost people will experience a traumatic event within their lifetime. One commonly recognized response to trauma exposure is posttraumatic stress disorder (PTSD). The biological underpinnings of PTSD, including epigenetic mechanisms of DNA methylation and gene expression, have been studied intensively. However, psychological posttrauma responses vary widely and can include positive outcomes, such as posttraumatic growth (PTG) and, more commonly, resilience. The aim of this systematic review was to summarize the current DNA methylation and gene expression data with respect to three potential posttrauma responses: PTSD, PTG, and resilience. A literature search identified 486 studies, 51 of which were deemed eligible for inclusion (total N = 10,633). All included studies examined PTSD and consistently implicated DNA methylation and gene expression changes in hypothalamic–pituitary–adrenal axis and inflammatory genes. Ten studies acknowledged resilience as a posttrauma response, but only two studies examined epigenetics and gene expression using a scale to measure resilience. Low resilience was associated with gene expression patterns in immune and dopamine genes, and high resilience was associated with a blunted inflammatory response. No studies examined epigenetic or gene expression changes associated with PTG. These findings highlight a focus on pathogenic research, which has failed to adequately acknowledge and measure positive posttrauma outcomes of PTG and resilience. Future research should examine DNA methylation and gene expression changes associated with PTG and resilience in addition to PTSD in order to gain a more comprehensive picture of an individual's well‐being following exposure to trauma.
Phenotypic, Transcriptional, and Functional Analysis of Liver Mesenchymal Stromal Cells and Their Immunomodulatory Properties
Authors: Taner Timucin , Abrol Nitin , Park Walter D. , Hansen Michael J. , Gustafson Michael P. , Lerman Lilach O. , van Wijnen Andre J. , Dietz Allan B. , Gores Gregory J. , Stegall Mark D. 
Published: January 2020

The liver is an immunologically active organ with a tolerogenic microenvironment at a quiescent state. The immunoregulatory properties of the liver appear to be retained after transplantation because liver allografts can reduce alloresponses against other organs that are simultaneously transplanted. Mechanisms of this phenomenon remain unknown. Given the known immunomodulatory properties of mesenchymal stromal cells (MSCs), we hypothesized that liver mesenchymal stromal cells (L‐MSCs) are superior immunomodulators and contribute to liver‐mediated tolerance. L‐MSCs, generated from human liver allograft biopsies, were compared with adipose mesenchymal stromal cells (A‐MSCs) and bone marrow mesenchymal stromal cells (BM‐MSCs). Trilineage differentiation of L‐MSCs was confirmed by immunohistochemistry. Comparative phenotypic analyses were done by flow cytometry and transcriptome analyses by RNA sequencing in unaltered cell cultures. The in vitro functional analyses were performed using alloreactive T cell proliferation assays. The transcriptome analysis showed that the L‐MSCs are different than the A‐MSCs and BM‐MSCs, with significant enrichment of genes and gene sets associated with immunoregulation. Compared with the others, L‐MSCs were found to express higher cell surface levels of several select immunomodulatory molecules. L‐MSCs (versus A‐MSCs/BM‐MSCs) inhibited alloreactive T cell proliferation (22.7% versus 56.4%/58.7%, respectively; P < 0.05) and reduced the frequency of interferon ɤ–producing T cells better than other MSCs (52.8% versus 94.4%/155.4%; P < 0.05). The antiproliferative impact of L‐MSCs was not dependent on cell‐to‐cell contact, could be reversed incompletely by blocking programmed death ligand 1, and required a higher concentration of the competitive inhibitor of indoleamine 2,3‐dioxygenase for complete reversal. In conclusion, L‐MSCs appear to be uniquely well‐equipped immunomodulatory cells, and they are more potent than A‐MSCs and BM‐MSCs in that capacity, which suggests that they may contribute to liver‐induced systemic tolerance.
Combined lymphovenous anastomosis and deep inferior epigastric perforator flap with lymphatic tissue preservation for defect reconstruction and lymphedema‐lymphocele prevention after medial thigh sarcoma resection: A case report
Authors: Scaglioni Mario F. , Meroni Matteo , Franchi Alberto , Fritsche Elmar 
Published: January 2020

AbstractSoft tissue sarcomas are a rare group of malignant tumors that often require an extensive surgical resection to be safely treated. When they are localized in the upper medial thigh, this treatment inevitably leads to large defects frequently causing a series of early and late postoperative complications. Among these, lymphocele and lymphedema are rather common and should try to be avoided. Many solutions with a demonstrated efficacy have been described for this purpose after groin dissection procedure, ranging from lymphovenous anastomosis to pedicled or free flaps. Anyway, there is much less information regarding the medial thigh. Here we present a case of resected sarcoma involving the adductors compartment reconstructed using a pedicled deep inferior epigastric (DIEP) flap with lymphatic tissue transfer, combined with preventive lymphovenous anastomosis (LVA) performed at the superior‐edge‐of‐the‐knee incision (SEKI) point. A 58‐year‐old patient presented a 10 cm × 12 cm soft tissue defect after margin free sarcoma removal. To fill this defect, we harvest a 24 × 9 cm pedicled DIEP flap conserving its lymphatic vessels running from the upper margin to the right groin lymphnodes. Then we rotated it maintaining the lymphnodes in their original site and moved it through an inguinal tunnel in the area of the defect. The distal part was de‐epithelized and folded down to cover the deeper region. The postoperative course was uneventful and at the 6 months follow up the patient showed a good outcome with no swelling and no signs of tumor relapse. This result therefore may suggest that this kind of combined treatment might be an effective technique to prevent all those complications linked to the impairment of lymphatic system drainage in the proximal medial thigh.