bluuebunny/crossref_metadata_2025_split huggingface dataset:
The unique expression profile of FAM19A1 in the mouse brain and its association with hyperactivity, long-term memory and fear acquisition
Authors: Yong Hyo Jeong , Ha Nui , Cho Eun Bee , Yun Seongsik , Kim Hyun , Hwang Jong-Ik , Seong Jae Young 
Published: March 2020

AbstractNeurodevelopment and mature brain function are spatiotemporally regulated by various cytokines and chemokines. The chemokine-like neuropeptide FAM19A1 is a member of family with sequence similarity 19 (FAM19), which is predominantly expressed in the brain. Its highly conserved amino acid sequence among vertebrates suggests that FAM19A1 may play important physiological roles in neurodevelopment and brain function. Here we used a LacZ reporter gene system to map the expression pattern of the FAM19A1 gene in the mouse brain. The FAM19A1 expression was observed in several brain regions starting during embryonic brain development. As the brain matured, the FAM19A1 expression was detected in the pyramidal cells of cortical layers 2/3 and 5 and in several limbic areas, including the hippocampus and the amygdala. FAM19A1-deficient mice were used to evaluate the physiological contribution of FAM19A1 to various brain functions. In behavior analysis, FAM19A1-deficient mice exhibited several abnormal behaviors, including hyperactive locomotor behavior, long-term memory deficits and fear acquisition failure. These findings provide insight into the potential contributions of FAM19A1 to neurodevelopment and mature brain function.
Comparison Of Mechanical And Chemical Nanocellulose As Additives To Reinforce Recycled Cardboard
Authors: Sanchez-Salvador Jose Luis , Balea Ana , Monte M. Concepción , Negro Carlos , Miller Meaghan , Olson James , Blanco Angeles 
Published: March 2020

AbstractRecycling cycles cause a decrease in mechanical paper properties due to cellulose fiber degradation. The use of cellulose micro/nanofibers (CMF/CNF) to reinforce paper strength has been well studied, although it has been found to have negative effects on drainage. However, the application of CMF/CNF as paper reinforcement is affected by the nanocellulose type. Thus in this study mechanical and chemical treatments in CNF production were compared. Old corrugated container (OCC) pulp used to produce recycled cartonboard was reinforced with 1) CMF from never-dried northern bleached softwood kraft pulp (NBSK) highly refined in a 16-inch low consistency refiner at 1200 rpm and 25 kW of net power; and 2) CNF from NBSK pulp treated by TEMPO-mediated oxidation and homogenization at 600 bars. CMF/CNF and OCC were pulped at the same time and handsheets formed with cationic starch (CS) as retention system. Mechanical, drainage and flocculation properties were evaluated and compared. Data were also compared with other sources of TEMPO CNF. Results show an improvement in mechanical properties, drainage and flocculation when OCC is reinforced with CMF obtained with LCR. Therefore, high fibrillation was not necessary to improve mechanical paper or cardboard properties.
Dietary prebiotics alter novel microbial dependent fecal metabolites that improve sleep
Authors: Thompson Robert S. , Vargas Fernando , Dorrestein Pieter C. , Chichlowski Maciej , Berg Brian M. , Fleshner Monika 
Published: March 2020

AbstractDietary prebiotics produce favorable changes in the commensal gut microbiome and reduce host vulnerability to stress-induced disruptions in complex behaviors such as sleep. The mechanisms for how prebiotics modulate stress physiology remain unclear; however, emerging evidence suggests that gut microbes and their metabolites may play a role. This study tested if stress and/or dietary prebiotics (Test diet) alter the fecal metabolome; and explored if these changes were related to sleep and/or gut microbial alpha diversity. Male F344 rats on either Test or Control diet were instrumented for electroencephalography biotelemetry measures of sleep/wake. After 5 weeks on diet, rats were either stressed or remained in home cages. Based on untargeted mass spectrometry and 16S rRNA gene sequencing, both stress and Test diet altered the fecal metabolome/microbiome. In addition, Test diet prevented the stress-induced reduction in microbial alpha diversity based on PD_Whole_Tree, which has been previously published. Network propagation analysis revealed that stress increased members of the neuroactive steroidal pregnane molecular family; and that Test diet reduced this effect. We also discovered links between sleep, alpha diversity, and pyrimidine, secondary bile acid, and neuroactive glucocorticoid/pregnanolone-type steroidal metabolites. These results reveal novel microbial-dependent metabolites that may modulate stress physiology and sleep.
PET imaging of medulloblastoma with an 18F-labeled tryptophan analogue in a transgenic mouse model
Authors: Xin Yangchun , Yue Xuyi , Li Hua , Li Zhiqin , Cai Hancheng , Choudhary Arabinda K. , Zhang Shaohui , Chugani Diane C. , Langhans Sigrid A. 
Published: March 2020

AbstractIn vivo positron emission tomography (PET) imaging is a key modality to evaluate disease status of brain tumors. In recent years, tremendous efforts have been made in developing PET imaging methods for pediatric brain tumors. Carbon-11 labelled tryptophan derivatives are feasible as PET imaging probes in brain tumor patients with activation of the kynurenine pathway, but the short half-life of carbon-11 limits its application. Using a transgenic mouse model for the sonic hedgehog (Shh) subgroup of medulloblastoma, here we evaluated the potential of the newly developed 1-(2-[18F]fluoroethyl)-L-tryptophan (1-L-[18F]FETrp) as a PET imaging probe for this common malignant pediatric brain tumor. 1-L-[18F]FETrp was synthesized on a PETCHEM automatic synthesizer with good chemical and radiochemical purities and enantiomeric excess values. Imaging was performed in tumor-bearing Smo/Smo medulloblastoma mice with constitutive actvation of the Smoothened (Smo) receptor using a PerkinElmer G4 PET-X-Ray scanner. Medulloblastoma showed significant and specific accumulation of 1-L-[18F]FETrp. 1-L-[18F]FETrp also showed significantly higher tumor uptake than its D-enantiomer, 1-D-[18F]FETrp. The uptake of 1-L-[18F]FETrp in the normal brain tissue was low, suggesting that 1-L-[18F]FETrp may prove a valuable PET imaging probe for the Shh subgroup of medulloblastoma and possibly other pediatric and adult brain tumors.
Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection
Authors: Mateos Jesús , Estévez Olivia , González-Fernández África , Anibarro Luis , Pallarés Ángeles , Reljic Rajko , Mussá Tufária , Gomes-Maueia Cremildo , Nguilichane Artur , Gallardo José M. , Medina Isabel , Carrera Mónica 
Published: March 2020

AbstractTuberculosis (TB) is the most lethal infection among infectious diseases. The specific aim of this study was to establish panels of serum protein biomarkers representative of active TB patients and their household contacts who were either infected (LTBI) or uninfected (EMI-TB Discovery Cohort, Pontevedra Region, Spain). A TMT (Tamdem mass tags) 10plex-based quantitative proteomics study was performed in quintuplicate containing a total of 15 individual serum samples per group. Peptides were analyzed in an LC-Orbitrap Elite platform, and raw data were processed using Proteome Discoverer 2.1. A total of 418 proteins were quantified. The specific protein signature of active TB patients was characterized by an accumulation of proteins related to complement activation, inflammation and modulation of immune response and also by a decrease of a small subset of proteins, including apolipoprotein A and serotransferrin, indicating the importance of lipid transport and iron assimilation in the progression of the disease. This signature was verified by the targeted measurement of selected candidates in a second cohort (EMI-TB Verification Cohort, Maputo Region, Mozambique) by ELISA and nephelometry techniques. These findings will aid our understanding of the complex metabolic processes associated with TB progression from LTBI to active disease.
The activation of BAFF/APRIL system in spleen and lymph nodes of Plasmodium falciparum infected patients
Authors: Dechkhajorn Wilanee , Benjathummarak Surachet , Glaharn Supattra , Chaisri Urai , Viriyavejakul Parnpen , Maneerat Yaowapa 
Published: March 2020

AbstractPrevious studies have reported activation of the B cell-activating factor (BAFF)/a proliferation-inducing ligand (APRIL) system in T independent immunity against malaria infection. Plasmodium falciparum (P. falciparum) infected animal model is not feasible. Therefore, little is known about the occurrence of BAFF/APRIL system and changes in falciparum lymphoid tissues. This study aimed to investigate the expression of BAFF/APRIL system components in lymphoid tissues from P. falciparum infected patients. Spleen and lymph node samples from 14 patients were collected at autopsy. Normal spleens and bacterially infected tonsils served as controls. The protein and/or mRNA expression of BAFF/APRIL and their cognate receptors, BAFF-R, TACI and BCMA, were determined by immunohistochemistry and RT-qPCR, respectively. The spleens of the patients exhibited significantly higher BAFF-R protein expression than normal spleens. Although without appropriate control, BCMA protein was markedly observed only in the lymph nodes. BAFF and BCMA mRNA levels were also significantly elevated in the spleen tissues of the patients compared with normal spleens. The overall BAFF-R protein levels in the lymphoid tissues of the patients correlated positively with parasitaemia. These findings are the first to confirm that BAFF/APRIL system activation in lymphoid tissues and is positively correlated with the parasitaemia levels in falciparum malaria.
Modification of radiosensitivity by Curcumin in human pancreatic cancer cell lines
Authors: Schwarz Katharina , Dobiasch Sophie , Nguyen Lily , Schilling Daniela , Combs Stephanie E. 
Published: March 2020

AbstractPancreatic cancer is one of the most aggressive malignancies and is characterized by a low 5-year survival rate, a broad genetic diversity and a high resistance to conventional therapies. As a result, novel therapeutic agents to improve the current situation are needed urgently. Curcumin, a polyphenolic colorant derived from Curcuma longa root, showed pleiotropic influences on cellular pathways in vitro and amongst others anti-cancer properties including sensitization of tumor cells to chemo- and radiation-therapy. In this study, we evaluated the impact of Curcumin on the radiosensitivity of the established human pancreatic cancer cell lines Panc-1 and MiaPaCa-2 in vitro. In contrast to MiaPaCa-2 cells, we found a significant radiosensitization by Curcumin in the more radioresistant Panc-1 cells, possibly caused by cell cycle arrest in the most radiation-sensitive G2/M-phase at the time of irradiation. Furthermore, a significant enhancement of radiation-induced apoptosis, DNA-double-strand breaks and G2/M-arrest after curcumin treatment was observed in both cell lines. These in vitro findings suggest that especially patients with more radioresistant tumors could benefit from a radiation-concomitant, phytotherapeutic therapy with Curcumin.
Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors
Authors: Stojsavljević Aleksandar , Vujotić Ljiljana , Rovčanin Branislav , Borković-Mitić Slavica , Gavrović-Jankulović Marija , Manojlović Dragan 
Published: March 2020

AbstractThe pathogenesis of malignant brain tumors (MBTs) should be better understood due to the evident association between prolonged exposure to metals and increased risk of MBTs. The present research aimed to find trace metals that could contribute to the pathogenesis of MBTs. Essential trace elements (Mn, Co, Zn, Cu, Se) and relevant toxic metals (Al, Ni, As, Sr, Cd, Ce, Pt, Pb, U) in the serum, cell fraction (CF), cerebrospinal fluid (CSF) and cancerous tissue (CT) samples of MBT patients were analyzed. The results were compared with sex- and age-matched control groups. For the first time, this research showed that elemental profiles of serum, CF, CSF and CT samples in MBT patients were significantly altered compared to the appropriate controls, as well as that higher contents of trace elements (particularly Mn, Se, and Pb) could be involved in the pathogenesis of MBTs. However, the most noticeable change found was the elevated U content, indicating its considerable role as a major cerebral discriminator of the presence/absence of MBTs. The U/Se ratio could be considered as an appropriate blood marker in diagnostic MBT evaluation. The reported results could contribute to better understanding of the poorly understood pathogenesis of MBTs. Furthermore, the reported results could highlight a molecular basis for the pathophysiological changes caused by the hazardous effects of trace metals on brain homeostasis.
A multilayer temporal network model for STD spreading accounting for permanent and casual partners
Authors: Vajdi Aram , Juher David , Saldaña Joan , Scoglio Caterina 
Published: March 2020

AbstractSexually transmitted diseases (STD) modeling has used contact networks to study the spreading of pathogens. Recent findings have stressed the increasing role of casual partners, often enabled by online dating applications. We study the Susceptible-Infected-Susceptible (SIS) epidemic model –appropriate for STDs– over a two-layer network aimed to account for the effect of casual partners in the spreading of STDs. In this novel model, individuals have a set of steady partnerships (links in layer 1). At certain rates, every individual can switch between active and inactive states and, while active, it establishes casual partnerships with some probability with active neighbors in layer 2 (whose links can be thought as potential casual partnerships). Individuals that are not engaged in casual partnerships are classified as inactive, and the transitions between active and inactive states are independent of their infectious state. We use mean-field equations as well as stochastic simulations to derive the epidemic threshold, which decreases substantially with the addition of the second layer. Interestingly, for a given expected number of casual partnerships, which depends on the probabilities of being active, this threshold turns out to depend on the duration of casual partnerships: the longer they are, the lower the threshold.
Automatic human identification based on dental X-ray radiographs using computer vision
Authors: Heinrich Andreas , Güttler Felix V. , Schenkl Sebastian , Wagner Rebecca , Teichgräber Ulf K.-M. 
Published: March 2020

AbstractA person may be identified by comparison between ante- and post-mortem dental panoramic radiographs (DPR). However, it is difficult to find reference material if the person is unknown. This is often the case when victims of crime or mass disaster are found. Computer vision can be a helpful solution to automate the finding of reference material in a large database of images. The purpose of the present study was to improve the automated identification of unknown individuals by comparison of ante- and post-mortem DPR using computer vision. The study includes 61,545 DPRs from 33,206 patients, acquired between October 2006 and June 2018. The matching process is based on the Speeded Up Robust Features (SURF) algorithm to find unique corresponding points between two DPRs (unknown person and database entry). The number of matching points found is an indicator for identification. All 43 individuals (100%) were successfully identified by comparison with the content of the feature database. The experimental setup was designed to identify unknown persons based on their DPR using an automatic algorithm system. The proposed tool is able to filter large databases with many entries of potentially matching partners. This identification method is suitable even if dental characteristics were removed or added in the past.
Anterior relapse or posterior drift after intraoral vertical ramus osteotomy
Authors: Rokutanda Satoshi , Yamada Shin-Ichi , Yanamoto Souichi , Sakamoto Hiroshi , Furukawa Kohei , Rokutanda Hiromi , Yoshimi Tomoko , Nakamura Takuya , Morita Yukiko , Yoshida Noriaki , Umeda Masahiro 
Published: March 2020

AbstractThis study aimed to evaluate the factors contributing to postoperative anterior relapse or posterior drift of the distal segment after intraoral vertical ramus osteotomy. A retrospective cohort study was conducted which included 31 patients who underwent setback surgery for mandibular prognathism by the intraoral vertical ramus osteotomy technique. Uni- and multivariate analyses were performed to determine the association of potential explanatory variables (sex, age, magnitude of setback, differences in setback magnitude between sides (right/left), duration of splint use, Angle’s classification of malocclusion, mandibular angle, and tightness of occlusion of the molars) with positional changes in the distal segment. The setback magnitude was only significant factor affecting (P = 0.015) for posterior drift, with significant posterior in setback magnitudes of less than 7.25 mm. Posterior drift after intraoral vertical ramus osteotomy is less likely if setback magnitude exceeds 7.25 mm. For setbacks less than 7.25 mm, posterior drift should either be carefully corrected postoperatively, or an alternative surgical technique should be used. The setback magnitude showed a significant association with the risk of posterior drift following intraoral vertical ramus osteotomy, and the determined cut-off value may serve as a predictor for postoperative outcomes.
Effects of engineered aluminum and nickel oxide nanoparticles on the growth and antioxidant defense systems of Nigella arvensis L.
Authors: Chahardoli Azam , Karimi Naser , Ma Xingmao , Qalekhani Farshad 
Published: March 2020

AbstractThe effects of different concentrations (0, 50,100, 1000 and 2500 mg/L) of engineered aluminum and nickel oxide nanoparticles (Al2O3 and NiO NPs) on plant growth, oxidative stress and antioxidant activities in the hydroponically grown tissues of Nigella arvensis L. were investigated. The plant biomass was significantly increased under 50 and 100 mg/L of Al2O3 NPs or 50 mg/L of NiO NPs treatment, but was significantly decreased at higher concentrations of these nanoparticles. Assays of several enzymatic antioxidants such as ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) in roots and shoots indicate a general increase of activities after exposure to 50–2,500 mg/L of Al2O3 NPs and NiO NPs. The results are corroborated by an increased 2,2-diphenyl-1-picryl hydrazyl (DPPH) scavenging activity, total antioxidant capacity, total reducing power, total iridoids content, total saponin content, and total phenolic content in treated plants by Al2O3 NPs compared to the control plants. By contrast, the antioxidant activities, formation of secondary metabolites, and other related physiological parameters such as the total antioxidant capacity, DPPH scavenging activity and total saponin content were inhibited after the concentration of NiO NPs was increased to 100 mg/L. Total phenols, saponins, iridoids and total antioxidant content and DPPH scavenging activity were increased in plants treated with 100–2,500 mg/L Al2O3 NPs. Overall, these two nanoparticles displayed different effects in the shoots and roots of plants at different concentrations, which may be due to their physico-chemical properties.
Enzymatic decontamination of paraoxon-ethyl limits long-term effects in planarians
Authors: Poirier Laetitia , Plener Laure , Daudé David , Chabrière Eric 
Published: March 2020

AbstractOrganophosphorus compounds (OP) are highly toxic molecules used as insecticides that inhibit cholinesterase enzymes involved in neuronal transmission. The intensive use of OP for vector control and agriculture has led to environmental pollutions responsible for severe intoxications and putative long-term effects on humans and wild animals. Many in vivo models were studied over the years to assess OP acute toxicity, but the long-term effects are poorly documented. Planarian, a freshwater flatworm having a cholinergic system, has emerged as a new original model for addressing both toxicity and developmental perturbations. We used Schmidtea mediterranea planarians to evaluate long-term effects of paraoxon-ethyl at two sublethal concentrations over three generations. Toxicity, developmental perturbations and disruption of behavior were rapidly observed and higher sensitivity to paraoxon-ethyl of next generations was noticed suggesting that low insecticide doses can induce transgenerational effects. With the view of limiting OP poisoning, SsoPox, an hyperthermostable enzyme issued from the archaea Saccharolobus solfataricus, was used to degrade paraoxon-ethyl prior to planarian exposure. The degradation products, although not lethal to the worms, were found to decrease cholinesterase activities for the last generation of planarians and to induce abnormalities albeit in lower proportion than insecticides.
Constraints on the Structure of the Shallow Crust in Central Italy from Geophysical Log Data
Authors: Montone Paola , Mariucci Maria Teresa 
Published: March 2020

AbstractTo better define the seismic velocities of the shallow crust in central Italy, in the area affected by the 1997 Colfiorito, 2009 L’Aquila and 2016–2018 Amatrice–Norcia seismic sequences, we selected all deep wells with available sonic logs from the Apennine belt to the related Adriatic foredeep. Sonic logs are among the most important in situ measurements of rock properties and provide a reliable image of physical conditions at depth. By analysing the wave train transit times, we inferred the P-wave velocity within depth intervals displaying homogeneous sonic log properties, and estimated the rock density by applying an empirical relationship between the sonic velocity and density in sedimentary rocks. We compared these results with the main litho-stratigraphic units in stratigraphic profiles of the wells. From the density estimates, we inferred the trends of the vertical stress magnitude in the belt, eastern front and foredeep geodynamic domains. This work is a contribution to better interpretation of physical conditions at depth and provides data that can be applied to define more complete seismological, gravity and magnetic models. We provide data uncertainties that must be considered to ensure proper use of data and to evaluate the spatial resolution of the models derived from those data.