bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Breast Cancer Videos on Youtube in the Arab World&nbsp;
Authors: Ayoub Georges , Chalhoub Elie , Sleilaty Ghassan , Kourie Hampig Raphael 
Published: September 2020

Abstract
BackgroundPatients are increasingly using YouTube™ as a source of information on Breast cancer, the most frequent cancer among women. Educating patients is highly important to reduce mortality rates. This study aims to evaluate, for the first time, Breast Cancer Videos on YouTube™ in the Arab world that hosts their highest use.MethodsIn this cross-sectional study, the most viewed 60 videos were evaluated for global quality (GQS score), reliability (modified DISCERN score), usefulness (content-specific score), and misleading claims. Videos’ power index, popularity, duration, and viewers' interaction were assessed. Sources (professional/ non-professional) and speakers (physician/ non-physician) were categorized.ResultsThe median Global Quality (3/5), Reliability (2/5), and Usefulness scores (4/11) were overall low. Out of all videos, the speaker was a physician in 32% and misleading information was found in 42%. Although professional source videos (45%) were less viewed, they were less misleading, of higher quality, reliability, and usefulness than non-professional source videos (55%). Source categories did not affect viewers' interaction, video power index, nor duration. While Symptoms (55%) were discussed the most, genetic counseling (13%) and prevention (20%) were scarcely mentioned; professionals were more likely to highlight early diagnosis importance. ConclusionsYouTube™ is poorly informational on breast cancer and may be inaccurate in the Arab world where highly used. Although professional uploaders’ videos tend to be more adequate, they are of lower quantity and popularity. Governments and physicians should upload more intelligibly informational videos, guide the public for accurate sources, and encourage regulations.
Content Analysis of Digital Media Coverage of the Human Papillomavirus Vaccine School-Entry Requirement Policy in Puerto Rico
Authors: Colón-López Vivian , Rivera-Figueroa Vilnery , Arroyo-Morales Glizette O , Medina-Laabes Diana T , Soto-Abreu Roxana , Rivera-Encarnación Manuel E , Díaz-Miranda Olga L , Ortiz Ana P , Wells Katelyn B , Vázquez-Otero Coralia , Hull Pamela C 
Published: September 2020

Abstract
BackgroundIn August 2018, Puerto Rico (PR) became the 4th state or territory in the United States to adopt a human papillomavirus (HPV) vaccine school-entry requirement, for students 11-12 years old. Evidence suggests that the content of media coverage may impact people's perception of HPV vaccine and their willingness to vaccinate. This study aimed to analyze the content of digital news coverage related to the implementation of the policy in PR.MethodsA content review was conducted of digital media published from January 2017 through December 2018. The content reviewed was carried out in two steps: 1) creating a matrix to summarize each article’s content about the policy and 2) qualitative analysis using a grounded theory approach. ResultsThe search resulted in 34 articles in 17 online local and international news outlets that reported on the implementation of the policy. Analyses showed that 61% of the news articles did not mention the number of required doses, and 79% discussed the new policy concerning cancer prevention. In 2017, news coverage focused mostly on describing the policy, while 2018 coverage focused on controversies surrounding the implementation. Negative emergent codes included: 1) infringement to patient and parental autonomy; 2) Hesitancy from the political sector 3) Hesitancy from groups and coalitions. Positive content included: 1) knowledge and acceptance of HPV vaccine for cancer prevention; 2) importance of education and protective sexual behaviors; and 3) new vaccination law proposal. Neutral emergent codes included: 1) Description of the policy; 2) Information about HPV related cancers; and 3) General information about HPV vaccine.ConclusionsMost of the media coverage in PR was neutral and included limited information related to the vaccine, HPV, and HPV-related cancers. Neutral and negative themes could influence public concerns regarding the new policy, as well as HPV vaccination rates in PR.
Inverse Correlation of miR-27a-3p&nbsp;and CDH5&nbsp;Expression Serves as a Diagnostic Biomarker of Proliferation and Metastasis of Clear Cell Renal Carcinoma
Authors: Wang Yifang , Zhou Xiaohui , Han Peipei , Lu Yunliang , Zhong Xuemin , Yang Yanping , Li Danping , Liu Deling , Li Qiuyun , Pan Nenghui , Mo Yingxi , Luo Wenqi , Li Ping , Zhou Xiaoying , Matskova Liudmila 
Published: September 2020

Abstract
Background: Cadherin-5 (CDH5) is aberrantly expressed in a variety of human cancers and plays an important role in angiogenesis. But its role in renal clear cell carcinoma (ccRCC) remains to be understood. Methods: The dysregulation of CDH5 expression in ccRCC and its association with clinicopathological characteristics were analyzed using the TCGA database. A meta-analysis was performed to verify the alteration of CDH5 expression in ccRCC using the GEO database. Quantitative RT-PCR and immunohistochemical staining were applied to assess the transcriptional and protein levels of CDH5. TargetScan and Tarbase were employed to predict the miRNAs with the potential to target mRNA of CDH5.Results: The mRNA level of CDH5 in ccRCC was significantly higher than in normal tissue. CDH5 mRNA expression could therefore serve as a potential diagnostic biomarker for ccRCC (AUC=0.844). However, the reduced CDH5 transcription levels were significantly correlated with patients in the T3-4 stage, lymph node and distant metastasis, as well as with a worse clinical outcome. We further observed that CDH5, at the protein level, was almost absent in ccRCC samples. In addition, a few databases screen showed that mir-27a-3p, is a highly conserved miRNA targeting CDH5. The expression of mir-27a-3p was significantly elevated in ccRCC tissues in contrast to normal tissues. Importantly, it was positively associated with the T3-4 stage and M stage, respectively, suggesting that the expression level of mir-27a-3p could serve as a diagnostic biomarker for ccRCC (AUC=0.775).Conclusion: Our data suggest that the reduced translational levels of CDH5 in ccRCC was related to the overexpression of mir-27a-3p. The higher mir-27a-3p and lower CDH5 expression significantly correlated with advanced clinical stages for ccRCC patients.
Retrospective PET/CT Imaged Based Dosimetry Following Transarterial Radioembolization
Authors: Thompson Briana C. , Dezarn William A. 
Published: September 2020

Abstract
Background: Transarterial Radioembolization (TARE) uses beta particle emitting microspheres, containing Yttrium 90 (90Y), to treat unresectable primary and metastatic liver tumors. Current dosimetry models are highly simplistic. 90Y post-TARE PET/CT images are being evaluated for their use in image based dosimetry post treatment allowing for improved treatment efficacy. We sought to provide guidance on reconstruction and dose calculation algorithms that give the most reliable image based dosimetry.Methods: Retrospective image study for 11 patients each having a 90Y PET/CT following TARE SIR-Spheres® treatment. In total, 6 emission images were reconstructed using Ordered Subset Expectation Maximization (OSEM) routines and Bayesian penalized-likelihood reconstruction algorithms (Q. Clear). PET/CT image sets were resampled to 3mm resolution and used to create voxel based dose distributions using our own convolution algorithm (WFBH), SurePlanTM MIRD and SurePlanTM LDM. Additionally, we analyzed the effects of a systematic personalized background subtraction prior to dose distribution creation. Results: Reconstructed activity was highest among Q. Clear β = 350 and 1000 and both OSEM methods. OSEM with 3 iterations and 24 subsets gave comparable dose distributions to Q. Clear β=1000 (max dose ratio of 0.96 ± 0.14). No statistical difference was identified among Q. Clear and OSEM methods comparing dose distributions with and without additional background subtraction post-reconstruction (average local gamma value = 98.01% ± 3.75%). LDM is more sensitive than MIRD to small activity differences between reconstruction methods (p-value= 0.048). LDM calculated max dose values were higher than MIRD. DVH curves showed LDM model potentially underestimates low dose values and overestimates high dose values.Conclusion: Background subtraction is sufficiently addressed in PET image reconstruction. We recommend post-TARE image based dosimetry to be calculated using image series reconstructed with Q. Clear β= 1000 or OSEM 3 iterations 24 subsets and using a convolution voxel based dose algorithm, such as SurePlanTM MIRD. Since more sophisticated corrections are not available at present, the 90Y activity distribution should be scaled by the local clinic’s activity recovery fraction, which also will directly scale the calculated dose distribution.
In vivo activation of pH-responsive oxidase-like graphitic-nanozymes for selective killing of Helicobacter pylori
Authors: Zhang Lufeng , Zhang Liang , Deng Hui , Li Huan , Tang Wentao , Guan Luyao , Qiu Ye , Donovan Michael , Chen Zhuo , Tan Weihong 
Published: September 2020

Abstract
Helicobacter pylori infection is a major etiological factor in various gastric diseases. However, clinical antibiotic triple therapy for H. pylori often encounters such critical problems as continuously decreased therapeutic efficacy and symbiotic bacteria experiencing severe side effects. Herein, we developed an in vivo activatable pH-responsive graphitic nanozyme, PtCo@Graphene (PtCo@G), for the selective treatment of H. pylori infections. Such nanozymes can resist gastric acid corrosion and activate oxidase-like activity to stably generate reactive oxygen species only in the acidic gastric milieu which endows them with superior selective bactericidal property. C18-PEGn-Benzeneboronic acid molecules were further modified on the PtCo@G, improving its targeting capability to H. pylori. Under acidic gastric pH, graphitic nanozymes showed notable bactericidal activity toward H. pylori, while no bacterial killing was observed under the intestinal condition. In the H. pylori-infected mouse model, high antibacterial capability toward H. pylori and negligible side effects toward normal tissues and symbiotic bacteria were also achieved. This graphitic nanozyme performs the desired enzyme-like activity at corresponding physiological sites and may address critical issues in the clinical treatment of H. pylori infections.
Postcranial evidence of late Miocene hominin bipedalism in Chad
Authors: Guy Franck , Daver Guillaume , Mackaye Hassane Taisso , Likius Andossa , Boisserie Jean-Renaud , Moussa Abderamane , Vignaud Patrick , Nekoulnang Clarisse 
Published: September 2020

Abstract
Terrestrial bipedal locomotion is one of the key adaptations defining the hominin clade. Evidences of undisputed bipedalism are known from postcranial remains of late Miocene hominins as soon as 6 Ma in eastern Africa. Bipedality of Sahelanthropus tchadensis was hitherto documented at 7 Ma in central Africa (Chad) by cranial evidence. Here, we present the first postcranial evidence of the locomotor behavior of the Chadian hominin with new insights on bipedalism at the early stage of our evolutionary history. The original material was discovered at locality TM 266 (Toros-Menalla fossiliferous area), and consists in one left femur and two antimeric ulnae. These new findings confirm that hominins were already terrestrial biped relatively soon after the human-chimpanzee divergence but also suggest that careful climbing arboreal behaviors was still a significant part of their locomotor repertoire.
Spatial control of potato tuberisation by the TCP transcription factor BRANCHED1b
Authors: Nicolas Michael , Torres-Perez Rafael , Wahl Vanessa , Rodríguez-Buey María Luisa , Cruz-Oró Eduard , Oliveros Juan , Prat Salomé , Cubas Pilar 
Published: September 2020

Abstract
The control of carbon allocation, storage and usage is critical for plant growth and development and is exploited for both crop food production and CO2 capture. Potato tubers are natural carbon reserves in the form of starch that have evolved to allow propagation and survival over winter. They form from stolons, below ground, where they are protected from cold temperatures and animal foraging. We show that BRANCHED1b (BRC1b) acts as a tuberisation repressor in aerial axillary buds, which prevents buds from competing in sink strength with stolons. BRC1b loss of function leads to ectopic production of aerial tubers and reduced underground tuberisation. In buds, BRC1b promotes dormancy, ABA signalling and downregulation of plasmodesmata gene expression. This limits sucrose unloading and access of the tuberigen factor SP6A to axillary buds. Moreover, BRC1b directly interacts with SP6A and blocks its tuber-forming activity in aerial nodes. Altogether these actions help promote tuberisation underground.
A Retrospective Study on Recovery of Joint Function of Distal Radial Fracture After Treated with Wei’s Traumatology Guidance combined with Limbs Fumigation Fang
Authors: Yan Wei , Kong Bo , Miao Zhengyue , Xi Xibing , Jiang Tao , Wu Changgui , Tian Hao 
Published: September 2020

Abstract
Objective: To explore the clinical effect on recovery of joint function of distal radial fracture after treated with Wei’s Traumatology Guidance combined with Limbs Fumigation Fang.Methods: A retrospective analysis was conducted on 84 patients with distal radius fracture. The cases were collected from October 2017 to July 2018 in Ruijin Hospital Affiliated to Medical College of Shanghai Jiao tong University. The fractured radius of patients was firstly fixed and then treated with Wei's traumatology guidance combined with Limbs Fumigation Fang. After that, the G-W wrist function, self-rating scale (PRWE) and simplified sf-36 health scale were used to analyze the recovery of joint function of distal radial fracture before and after treatment.Results: There were significant differences in wrist function evaluation, wrist patient self-rating scale (PRWE) and simplified SF-36 health scale before and after treatment (P < 0.05).After treated with Wei’s Traumatology Guidance combined with Limbs Fumigation Fang for 3 weeks, the wrist pain, range of motion, strength and quality of life were improved a lot than before. Conclusion: Wei’s Traumatology Guidance combined with Limbs Fumigation Fang is an ideal way to restore wrist function with definite curative effect and high safety.
A Bifunctional Cytochrome P450 Enzyme Involved in the O-Dealkylation or N-Dealkoxymethylation of Chloroacetanilide Herbicides in Rhodococcus sp. B2
Authors: Liu Hongming , Yuan Meng , Liu Aimin , Zhu Guo-Ping , Sun Li-Na 
Published: September 2020

Abstract
Background: The chloroacetamide herbicides pretilachlor is an emerging pollutant, Due to the large amount of use, its presence in the environment threatens to human health. However, the molecular mechanism of pretilachlor degradation is unknown. Results: Now, Rhodococcus sp. B2 was isolated from rice field and found to degrade pretilachlor. The maximum pretilachlor degradation efficiency (86.1%) was observed at a culture time of 5 d, 50 mg/L initial substrate, pH 6.98, and 30.1°C. One novel metabolite was identified by gas chromatography-mass spectrometry (GC-MS). Draft genome comparison demonstrated that a 32,147-bp DNA fragment, comprising a gene cluster (EthRABCDB2), was absent in the mutant strain TB2 which could not degrade pretilachlor. The Eth gene system, encodes an AraC/XylS family transcriptional regulator, a ferredoxin reductase, a cytochrome P-450 monooxygenase, a ferredoxin and a 10-kDa unknown protein. Complementation of EthABCDB2 and EthABDB2, but not EthABCB2 in strain TB2 restored its activity against chloroacetamide herbicides. The codon of EthABCDB2 was optimized, expressed in Escherichia coli, and purified utilizing Ni-affinity chromatography. A mixture of EthABCDB2 or EthABDB2 but not EthABCB2 catalyzed the N-dealkoxymethylation activity toward alachlor, acetochlor, butachlor, and propisochlor and O-dealkylation activity toward pretilachlor, revealing that EthD acted as a ferredoxin in strain B2. EthABDB2 displayed maximal activity at 30 °C and pH 7.5. Conclusions: This is the first report of a P450 family oxygenase catalyzing the O-dealkylation or N-dealkoxymethylation of pretilachlor and propisochlor. And the results provide microbial resource for the remediation of chloroacetamide herbicides-contaminated sites,
Early Outcomes of Pelvic Trauma Patients – Complications, ICU Stay and Treatment Concepts in Two ATLS® Compliant Trauma Centers: Germany and Qatar
Authors: Keil Holger , El-Menyar Ayman , Daerr Julian , Weil Frederik , Abdelrahman Husham , Alhammoud Abduljabbar , Ghouri Syed I , Babikir ElHadi , Wahlen Bianca M , Al-Thani Hassan , Grützner Paul A , Münzberg Matthias 
Published: September 2020

Abstract

Background: Pelvic trauma causes severe threats especially to polytraumatized patients. Not only it is in itself a possible cause for significant bleeding but it also indicates a high risk for intra-abdominal injuries. The initial treatment of patients with pelvic trauma follows the ATLS principles of priority-oriented treatment. To examine the value of this highly standardized concept and to evaluate the effect of different patient collectives on early outcome parameters, two large collectives from Germany and Qatar were analyzed regarding injury parameters and early outcomes.Methods: Patients were recruited in Hamad General Hospital, Doha, Qatar (HGH) and BG Trauma Center Ludwigshafen, Germany (BG). All patients that were treated with a pelvic fracture between 2013 and 2016 were included in this retrospective analysis. Demographic parameters were collected as well as type of injury and the frequency of complication parameters as pneumonia, acute kidney failure, ARDS, sepsis and amount of blood transfusion.Results: We recruited 1436 patients with pelvic fracture (645 from BG and 791 from HGH). The mean age was 57.4 years in the BG and 33.6 years in the HGH group (p<0.000). The mean ISS was 17.81 in the BG and 15.88 in the HGH group (p=0.009). The mean pelvic AIS was 2.65 in the BG and 2.25 in the HGH group (p<0.000). The mean frequency of complications was 9.3% in the BG and 9.9% in the HGH group (p=0.128). The mean frequency of ARDS was significantly higher in the BG group than in the HGH group (5.6% vs. 1.8%, p<0.000). The mean frequency of blood transfusion was significantly lower in the BG group than in the HGH group (28.8% vs. 39.2%, p<0.000).Discussion and Conclusion: Despite significant differences in the two collectives, this analysis shows comparable results regarding early outcome parameters in patients with pelvic injuries. In total, pelvic injuries are accompanied by a relatively high complication risk and need to be evaluated and treated according to priority-based algorithms.
Factors Influencing MLS Career Choice Amid the COVID-19 Pandemic: A&nbsp;Cross-Sectional Study
Authors: Schwartz Lisa , Cymrot Cliff 
Published: September 2020

Abstract
Background: The COVID-19 pandemic has had a significant impact on the healthcare workforce. Increased personal risk, job instability, and workplace demands due to COVID-19 may reduce individuals’ interests in pursuing a healthcare career. Yet the enhanced visibility of the critical role of healthcare professionals in addressing the crisis may have strengthened individuals’ resolve to serve others as a healthcare provider. Moving forward it will be important for higher education institutions to identify what factors may influence prospective students’ decisions to pursue a healthcare career, including medical laboratory sciences, so that recruitment efforts may be focused accordingly. Methods: A cross sectional study was conducted using a modified version of the Health Care Choice instrument to include questions regarding COVID-19. Seventy-two (n=72) students enrolled in undergraduate and graduate-level medical laboratory science programs at a university in the United States completed the survey. Parametric and non-parametric analyses were performed to identify relationships between factors that influence career choice and particular demographic factors, as well as the impact of COVID-19 on these factors. Results: Personal interests and nature of the work were the influencing factors rated highest, followed by job prospects. Prior healthcare exposure, self-efficacy, and social influences were rated lower. There were few significant differences based on demographic factors, except those who had military experience. Participants reported being personally impacted by the COVID-19 pandemic, but for most it did not change their career choice. Conclusions: Medical laboratory sciences students report the factors that influence their career choice are similar to the factors that impact other health professionals in choosing their career. Recruitment strategies should focus on the impact that medical laboratory sciences professionals have on people’s lives and the highly skilled, hands-on nature of the work, particularly during and after the COVID-19 pandemic.
ACTN1 Supports Tumor Growth by Inhibiting Hippo Signaling in Hepatocellular Carcinoma
Authors: Chen Qian , Zhou Xiao-Wei , Zhang Ai-Jun , He Kang 
Published: September 2020

Abstract
Background: Alpha actinins (ACTNs) are major cytoskeletal proteins and exhibit many non-muscle functions. Emerging evidence have uncovered the regulatory role of ACTNs in tumorigenesis, however, the expression pattern, biological functions, and underlying mechanism of ACTN1 in hepatocellular carcinoma (HCC) remain largely unexplored.Methods: Immunohistochemical analysis of a HCC tissue microarray (n = 157) was performed to determine the expression pattern and prognostic value of ACTN1 in HCC. In vitro loss-of-function study in HCC cells were carried out to investigate ACTN1 knockdown on cell proliferation. In vivo subcutaneous xenograft model and intrahepatic transplantation model were generated to decipher the contribution of ACTN1 in the tumor growth of HCC. Gene set enrichment analysis, quantitative real-time PCR, Co-immunoprecipitation, immunofluorescence and western blotting were performed to identify the underlying molecular mechanism.Results: It was found that ACTN1 was significantly upregulated in HCC tissues and closely related to llpha-fetoprotein level, tumor thrombus, tumor size, TNM stage and patient prognoses. Knockdown of ACTN1 suppressed in vitro cell proliferation and in vivo tumor growth of HCC cells. Mechanistically, knockdown of ACTN1 increased Hippo signaling pathway activity and decrease Rho GTPases activities. Mechanistically, ACTN1 could competitively interact with MOB1 and decrease the phosphorylation of LATS1 and YAP. The growth-promoting effect induced by ACTN1 was significantly abrogated by pharmacological inhibition of YAP with verteporfin or super-TDU.Conclusions: ACTN1 is highly expressed in HCC tissues and acts as a tumor promoter by suppressing Hippo signaling via physical interaction with MOB1. ACTN1 may serve as a potential prognostic marker and therapeutic target for HCC.