bluuebunny/crossref_metadata_2025_split huggingface dataset:
YouTube as a source of information about orthodontic clear aligners
Authors: Ustdal Gokay , Guney Ayca Ustdal 
Published: February 2020

ABSTRACT

Objectives
To evaluate the content, reliability, and quality of videos about orthodontic clear aligners on YouTube.

Materials and Methods
Researchers used the Google Trends website to determine that the most frequently used search term for orthodontic clear aligners on the Internet was: “Invisalign.” A search was then conducted on YouTube using the key word “Invisalign.” From the first 140 results, 100 videos were selected for analysis. A 13-point content score was used to classify poor-content and rich-content videos, and the global quality scale (GQS) was used to examine quality of the videos. To evaluate reliability of the information, a five-question scale was used. The Mann-Whitney U-test, χ2 test, and Pearson correlation coefficients were used for statistical evaluations.

Results
Of the YouTube videos, 33 were classified as rich content and 67 as poor content. Most videos (73%) were uploaded by laypeople, and most uploaders (71%) were women. The most commonly discussed content was instructions (65%), followed by procedure (57%) and pain (52%). Regarding the GQS, most of the videos were evaluated as moderate quality (51%). Compared with the poor-content video group, the rich-content video group had a significantly higher GQS score (P = .004). There was no significant difference between the poor-content and rich-content groups regarding information reliability (P &gt; .05).

Conclusions
Video content on YouTube relating to aligner orthodontics was generally insufficient. The quality of videos was moderate, but the reliability of information was generally poor. Specialists should refer patients to reliable sources of information.
Rebranding and its Impact on Banking Activities – Case Study
Authors: Kiss Marta 
Published: February 2020

Abstract
Rebranding is regarded as a necessary form when an organisation faces different problems due to the economic transformations of the society that requires new ways of visibility and decides to assign revitalization techniques to the brand image in order to refresh it. Nowadays, most of the Romanian banking companies went through the rebranding process firstly because they were involved in mergers and acquisitions, and secondly, to expand their target markets and to keep their loyal customers in an ever-changing market environment. This study aims to outline the impact of rebranding on a financial institution in Romania, CEC Bank, the oldest Romanian bank operating on the Romanian market since 1864. Following the 2007 rebranding, the purpose of the bank was to create a competitive commercial bank image. For this bank the rebranding process proved to be successful.
Genetic and reproductive toxicity of lamivudine, tenofovir disoproxil fumarate, efavirenz and their combination in the bone marrow and testicular cells of male mice
Authors: Bakare Adekunle A. , Akinseye Kehinde M. , Aminu Bayonle A. , Ofoegbu Francis C. , Anifowose Saheed O. , Abruda Stork , Fadoju Opeoluwa M. , Ogunsuyi Olusegun I. , Oyeyemi Ifeoluwa T. , Alabi Okunola A. , Adetona Olusola M. , Alimba Chibuisi G. 
Published: February 2020

Abstract
The combination of antiretroviral (ARV) drugs: lamivudine, tenofovir disoproxil fumarate (TDF) and efavirenz is among the preferred first-line regimens for adolescents and adults infected with HIV. However, knowledge on in vivo genetic and reproductive toxicity of each of these drugs and their combination is limited. We evaluated the genotoxicity of lamivudine, TDF, efavirenz and their combination utilizing the mouse micronucleus (MN) and sperm morphology tests. Histopathological analysis of the testes of exposed mice was also carried out. 0.016, 0.032, 0.064 and 0.129 mg/kg bwt of lamivudine, TDF and the combination; and 0.032, 0.064, 0.129 and 0.259 mg/kg bwt of efavirenz corresponding to 0.125, 0.250, 0.500 and 1.000 x the human therapeutic daily dose (HTD) of each of the ARVs and their combinations were administered to mice for 5 consecutive days. Data on MN showed a significant increase (p < 0.05) across the tested doses of TDF, efavirenz and the combination, with the combination inducing lower frequency of MN than TDF and efavirenz. Lamivudine did not evoke significant induction of MN. Significant increase in frequency of abnormal sperm cells were observed in the tested samples, however, the combination induced the highest number of abnormal spermatozoa. The ARVs and their combination induced pathological lesions such as vacuolation and necrosis in mice testes. These findings suggest that the individual ARVs and their combination are potentially capable of activating genetic alterations in the bone marrow and germ cells of male mice thereby raising concern for long term use by HIV patients.
The Possible Ways of Country Brand Communication and Their Classification Based on the POE Model
Authors: Papp-Váry Árpád , Farkas Máté 
Published: February 2020

Abstract
The number of marketing communications tools and channels is steadily increasing – in addition, this growth has been accelerated since the emergence of the Internet and social media. On the one hand, there is an increasing dilemma of which tools one should choose from the plentiful options, and, on the other hand, the (material, human, and time) resources devoted to this are limited. This is valid for the marketing communications of “classic” products, services, and countries. This review article attempts to present the variety of available options with the help of the POE (paid, owned, earned) model and describe their advantages and disadvantages. The novelty of the study is that it focuses on a review of country communication campaigns and initiatives. It offers various visual examples for the different components of the POE model, clarifying the paid media, owned media, and earned media options for country brand communication. It mostly uses examples from Hungary, but other countries’ good practices are also included. Finally, as a result of the analysis, the article summarizes the possible ways of country brand communication according to the POE model in a summary table, which may also contribute to the work of academics and practitioners in the field.
Digital Media Use of Older Adults in Mureş County, Romania
Authors: Vajda András , Tőkés Gyöngyvér Erika 
Published: February 2020

Abstract
This paper analyses the media practices of older adults from Mureş County (village and small town). The first part of this paper examines the integration of digital media into current society and everyday life along with the characteristics of the knowledge and skill acquisition related to digital media. The second half, grounded on empirical qualitative data, offers insight into the digital media practices of older people in Mureş County, Romania, as well as into their opportunities and the contexts regarding the knowledge and skill acquisition necessary for the use of digital media. The paper is based on an exploratory qualitative research aimed at offering insight into the Romanian situation, identifying the obstacles to the digital media use of the older people living in rural areas, and laying the groundwork for a more extended study.
Technical Performance and Perceived Exertion Variations Between Small-Sided Basketball Games in Under-14 and Under-16 Competitive Levels
Authors: Clemente Filipe Manuel , Sanches Rita , Moleiro Carlos Filipe , Gomes Mónica , Lima Ricardo 
Published: February 2020

Abstract
The aim of this study was twofold: i) to compare the rate of perceived exertion (RPE) and the frequencies of technical actions per minute in different small-sided games (SSGs) between under-14 and under-16 age groups, and ii) to compare the RPE and the frequencies of technical actions per minute between 1 x 1, 2 x 2, 3 x 3, 4 x 4 and 5 x 5 formats within age groups. Twenty young male basketball players from the same club (N = 10, from under-14; N = 10, from under-16) competing at the national level voluntarily participated in this study. Five different SSGs (1 x 1, 2 x 2, 3 x 3, 4 x 4 and 5 x 5) were played twice on courts of the same relative area and were compared in terms of the RPE and technical actions. The number of technical-tactical actions per minute, i.e. conquered balls (CB), received balls (RB), lost balls (LB), attacking balls/passes (AB), shots (S), rebounds (R), and the RPE were collected for each player for each SSG session. The results revealed that most of the differences between age groups were considered trivial/small and/or unclear for all SSG formats, though likely moderate differences between age groups were found in 1 x 1 and 2 x 2 SSGs, revealing that young players had greater frequencies of received, conquered, and lost balls. Within-age-group comparisons also showed moderate-to-large increases in technical actions during smaller formats than during larger ones. The main evidence of this study revealed that age group seemed not to largely influence the RPE or technical actions during different SSGs. However, smaller formats moderately-to-largely increased the number of technical actions. Interestingly, the biggest format (5 x 5) largely increased the RPE in comparison to the remaining formats. As a conclusion, technical actions and the RPE were influenced more by the format of play than by the age group.
Notational Comparison Analysis of Outdoor Badminton Men’s Single and Double Matches
Authors: Pérez-Turpin José Antonio , Elvira-Aranda Carlos , Cabello-Manrique David , Gomis-Gomis María José , Suárez-Llorca Concepción , Andreu-Cabrera Eliseo 
Published: February 2020

Abstract
The objectives of this study were to determine the results of the analysis of single and double outdoor badminton men’s matches and to determine the relationship between technical and tactical aspects in a study organized by the BWF (Badminton Word Federation), on a sand surface. Twenty men’s singles matches were recorded using video cameras and analysed with a Dartfish video analysis software package. Along with this, percentages of use of technical elements were analysed by comparing the different modalities. For the single format Lob, Clear, Drop, Smash, Drive were used, different from the double format that used Lob, Clear, Drop, Smash, Drive. The study confirmed the applicability of computerized notation analysis to determine the characteristics of Outdoor Badminton on sand.
Aplasia Cutis Congenita with Unusual Localization – Case Report
Authors: Chiriac Anca , Naznean Adrian , Podoleanu Cristian , Stolnicu Simona 
Published: February 2020

Abstract
Congenital skin aplasia, known more as aplasia cutis congenita (ACC), is a rare congenital disease, characterized by absence of the skin, observed since birth, more often affecting the scalp and rarely the trunk or limbs. We report here for the first time a non-syndromic localized ACC, characterized by a small solitary area of skin atrophy on the cervical area in a healthy 3-day-old female infant.
Ensuring Reliability of Control Data in Engineering Systems
Authors: Dychko A. , Yeremeyev I. , Kyselov V. , Remez N. , Kniazevych A. 
Published: February 2020

Abstract
The paper presents the approach of determination of rationality coefficients of control system, inputted uncertainty, control of the process, system errors, and uncertainty. Algorithms for identifying the states of system have been developed on the basis of theorems of identification. They actually implement the theoretical multiplication cross-section and establish that increasing the reliability of information is possible only through the use of redundancy (structural, procedural, and informational). The increase in the reliability of control data with the developed methods ensures significant improvement of the functioning of information systems and facilitates the adoption of more substantiated decision making.
Diffuse Cutaneous Mastocytosis (Pigmented Maculopapular Cutaneous Mastocytosis) in a Cat
Authors: Szczepanik Marcin , Wilkołek Piotr , Śmiech Anna , Taszkun Iwona 
Published: February 2020

Abstract
Diffuse cutaneous mastocytosis was diagnosed in a 6-year-old, indoor, neutered female domestic European shorthair cat. Marked pruritus located mainly on the head and neck was noticed in the cat and in this area the animal had developed alopecia, crusts, and plaques. Histologically, monomorphic mast cells were found in the superficial dermis and around the hair follicles. Mast cells were well differentiated, with central nuclei and granular cytoplasm, with metachromatic granules which stained positively with Toluidine blue stain. The animal was successfully treated with oclacitinib at a dose of 1 mg/kg, twice a day per os.
Variability of ex-vivo stimulated T-cells secretory profile in healthy subjects
Authors: Manescu Ion Bogdan , Manu Doina Ramona , Serban Georgiana Mihaela , Dobreanu Minodora 
Published: February 2020

Abstract
Peripheral blood lymphocytes (PBL) are able to synthesize various cytokines that play key roles in the immune response and intercellular signaling. Since alterations in cytokine production and/or activity occur in many pathological processes, the study of cytokine synthetic capacity of PBL is a valuable tool for assessing the immune profile. In this paper, we aimed to investigate the variability of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-α) and interferon gamma (IFN-γ) synthetic capacity of CD4+/CD8+ T-cells stimulated ex-vivo in healthy subjects, by means of a commercial intracellular cytokine staining (ICS) protocol. Peripheral blood mononuclear cells were isolated from 16 healthy subjects by Ficoll gradient centrifugation and activated ex-vivo with PMA/Ionomycin/Brefeldin-A for 4 hours. Activated PBL were surface-stained for CD3/CD4/CD8, fixed and permeabilized. ICS was performed using anti-human IL-2/TNF-α/IFN-γ and samples were analyzed on a BD-FACSAria-III flow cytometer. We recorded high post-isolation and post-activation mean viabilities: 82.1% and 82.4% respectively, p=0.84. Both CD4+/CD8+ subpopulations were found to partially produce each of the three cytokines, but in different proportions. On average, a significantly greater percentage of CD4+ cells was shown to produce IL-2 and TNF-α, compared with CD8+ cells (61.5%+/-5.8 vs. 25%+/-5.6 and 26.9%+/-11 vs. 7.5%+/-3.3 respectively, p---lt---0.0001 for both). Contrarily, IFN-γ was produced by a higher proportion of CD8+ cells (8.4%+/-3.9 vs. 6.8%+/-3.2, p=0.01). These results show that the employed ICS protocol elicits a satisfactory and consistent cytokine response from PBL of healthy subjects. The collected data may be used to outline a preliminary reference range for future studies on both healthy/pathological subjects.
The direct deleterious effect of Th17 cells in the nervous system compartment in multiple sclerosis and experimental autoimmune encephalomyelitis: one possible link between neuroinflammation and neurodegeneration
Authors: Balasa Rodica , Maier Smaranda , Barcutean Laura , Stoian Adina , Motataianu Anca 
Published: February 2020

Abstract
The processes of demyelination and neurodegeneration in the central nervous system (CNS) of multiple sclerosis (MS) patients and experimental autoimmune encephalomyelitis (EAE) are secondary to numerous pathophysiological mechanisms. One of the main cellular players is the Th17 lymphocyte. One of the major functions described for Th17 cells is the upregulation of pro-inflammatory cytokines, such as IL-17 at the level of peripheral and CNS inflammation. This review will focus on the newly described and unexpected, direct role played by the Th17 cells in the CNS of MS patients and EAE models. Th17 and their main cytokine, IL-17, are actively involved in the onset and maintenance of the immune cascade in the CNS compartment as Th17 were found to achieve brain-homing potential. Direct interaction of myelin oligodendrocyte glycoprotein - specific Th17 with the neuronal cells firstly induces demyelination and secondly, extensive axonal damage. The Th17 cells promote an inflammatory B cell response beyond the BBB through the presence of infiltrating Th follicles. Due to their role in preventing remyelination and direct neurotoxic effect, Th17 cells might stand for an important connection between neuroinflammation and neurodegeneration in a devastating disease like MS. The Th17 cell populations have different mechanisms of provoking an autoimmune attack not only in the periphery but also in the CNS of MS patients.
Gastric cancer is associated with a high rate of microsatellite instability versus chronic gastritis: A retrospective study
Authors: Xing Li , Guo Hua , Zheng Dongjie , Liu Jin , Zhou Baojun , Li Yanping , Wang Ning , Zhao Pu , Liang Yan , Wu Wenxin , Li Guixin 
Published: February 2020

Abstract

Objective: Microsatellite instability (MSI) in gastric cancer contributes to genetic complexities of gastric cancer. In the current study, we employed a panel of mononucleotide and dinucleotide markers to detect MSI in 99 gastric cancer patients and 91 chronic gastritis patients and further analyzed the association of MSI with clinicopathologic variables of the study patients.

Methods: We retrospectively analyzed the clinicopathologic data of primary gastric cancer patients and chronic gastritis patients. MSI was analyzed using five microsatellite markers, including D2S12, D5S346, D17S799, BAT26, and D18S34. MSI was defined as either a band shift or the appearance of a novel band in DNA. Multivariate logistic regression analysis was used to predict risk of MSI.

Results: Seventeen (17.2%) gastric cancer patients and 7 (7.7%) chronic gastritis patients were positive for MSI (P=0.012). Multivariate analysis further showed that gastric cancer was associated with a significantly higher likelihood for MSI versus gastritis (OR 3.73; 95% CI 1.19, 11.72; P=0.024) while age, drinking or smoking was not associated with increased MSI.

Conclusion: Gastric cancer is associated with a high rate of MSI. MSI should be further explored in future studies with a larger sample size for its role in gastric cancer development and as a predictive biomarker.
Identification of Susceptibility Genes in Hepatic Cancer Using Whole Exome Sequencing and Risk Prediction Model Construction
Authors: Zheng Jinghui , Tang Youming , Hou Encun , Bai Guangde , Lian Zuping , Xie Peisheng , Tang Weizhi 
Published: February 2020

Abstract

Objective: To identify the susceptible single nucleotide polymorphisms (SNPs) loci in HCC patients in Guangxi Region, screen biomarkers from differential SNPs loci by using predictors, and establish risk prediction models for HCC, to provide a basis of screening high-risk individuals of HCC.

Methods: Blood sample and clinical data of 50 normal participants and 50 hepatic cancer (HCC) patients in Rui Kang Hospital affiliated to Guangxi University of Traditional Chinese Medicine were collected. Normal participants and HCC patients were assigned to training set and testing set, respectively. Whole Exome Sequencing (WES) technique was employed to compare the exon sequence of the normal participants and HCC patients. Five predictors were used to screen the biomarkers and construct HCC prediction models. The prediction models were validated with both training and testing set.

Results: Two-hundred seventy SNPs were identified to be significantly different from HCC, among which 100 SNPs were selected as biomarkers for prediction models. Five prediction models constructed with the 100 SNPs showed good sensitivity and specificity for HCC prediction among the training set and testing set.

Conclusion: A series of SNPs were identified as susceptible genes for HCC. Some of these SNPs including CNN2, CD177, KMT2C, and HLADQB1 were consistent with the previously identified polymorphisms by targeted genes examination. The prediction models constructed with part of those SNPs could accurately predict HCC development.
A rare case of acute myeloid leukemia with ARHGEF12 (LARG, 11q23.3) and MAPRE1 (EB1, 20q11.21) fusion gene in an elderly patient
Authors: Macarie Ioan , Tripon Florin , Dorcioman Bogdana , Macarie Melania 
Published: February 2020

Abstract

Introduction. We report one elderly patient diagnosed with a rare subtype of acute myeloid leukemia (AML) and also with a very rare fusion gene involving ARHGEF12 (LARG, 11q23.3) and MAPRE1 (EB1, 20q11.21) genes.

Material and methods. Clinical examination and routine analysis were performed including peripheral blood smear, immunophenotyping of the peripheral blood by flow cytometry and several molecular analyses.

Results. Peripheral blood smear showed 80% blasts with round and some with convoluted nuclei, with basophilic cytoplasm, identified as monoblast and the majority of cells as promonocytes. Peripheral blood immunophenotyping was consistent with monocytic differentiation. Molecular analysis was negative for FLT3 ITD, FLT3 D835, NPM1, and DNMT3A R882 mutations. Multiplex ligation-dependent probe amplification revealed no copy number aberration. Ligation-dependent reverse transcription polymerase chain reaction (LD-RT-PCR) analysis identified the presence of one gene fusion between ARHGEF12 (LARG, 11q23.3) and MAPRE1 (EB1, 20q11.21) genes. The patient had no significant comorbidities, the renal function was normal and Eastern Cooperative Oncology Group performance status was 2 at diagnosis and 1 after treatment. She was treated with decitabine. She became transfusion independent and a reduction of the number of blasts was obtained.

Conclusions. The outcome of our AML patient was favorable but other patients with fusion genes involving ARHGEF12 (LARG, 11q23.3) and MAPRE1 (EB1, 20q11.21) should be reported, contributing to a better characterization of the disease, to monitor the minimal residual disease and in the end to more targeted treatment options. LD-RT-PCR represent a valuable multiplex technique for fusion gene analysis.
PENGARUH KEPUASAN KERJA, STRES KERJA, DAN LINGKUNGAN KERJA NON FISIK TERHADAP TURNOVER INTENTION
Authors: Rijasawitri Desak Putu , Suana I Wayan 
Published: February 2020

This study aims to examine the effect of job satisfaction, job stress, work environment on turnover intention. This research was conducted at PT. Kwalita Bali, Gianyar Regency. The number of respondents in this study were 35 people. Data collection in this study is by distributing questionnaires using a 5-point Likert Scale to measure 17 indicators and four research variables and the data analysis technique used in this study is multiple linear regression analysis. The results of this study prove that job satisfaction has a negative effect on turnover intention, meaning that the higher the level of employee job satisfaction, the lower the level of turnover intention. Furthermore, work stress has a positive effect on turnover intention, meaning that the higher the level of stress experienced by employees, the turnover intention will increase. Finally the non-physical work environment has a negative effect on turnover intention where the better and more comfortable the work environment, the lower the level of turnover intention.
Keywords: job satisfaction, work stress, work environment, turnover intention