bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changing managerial roles in Danish universities
Authors: Hansen Hanne Foss , Lind Jonas Krog , Stage Andreas Kjær 
Published: March 2020

AbstractThe article analyses changes in university managerial roles in the wake of a range of reforms, most notably a radical Danish management reform in 2003, using institutional work as the theoretical framework. Both qualitative and quantitative data is drawn upon, the former consisting of interviews with academics and managers on all levels and the latter in the form of payroll data for all Danish university employees. By combining these data in a mixed methods study, the analysis reveals how managerial roles have changed slowly, steadily, and substantially in the years since the reforms, resulting in extensive change. The article hereby questions the resilience of universities as organisational incarnations of a traditional collegial template.
Transition into Liminal Legality: DACA’s Mixed Impacts on Education and Employment among Young Adult Immigrants in California
Authors: Hamilton Erin R , Patler Caitlin , Savinar Robin 
Published: March 2020

Abstract
The Deferred Action for Childhood Arrivals (DACA) program, implemented by executive order in 2012, granted a subset of undocumented youth temporary relief from deportation, work authorization, and other benefits. While theories of immigrant integration predict that legalization will enable immigrant socioeconomic mobility, past research on DACA’s effects on education and employment have reached mixed conclusions, possibly reflecting the limitations of different methodological approaches to the question. Using multiple data sources and mixed methods, we analyzed both whether and how DACA impacted education and employment among undocumented immigrants in California. Our difference-in-differences analysis of the 2007–2017 waves of the California Health Interview Study employs a more precise definition of the DACA-eligible population than previous studies, yet we also find mixed effects. Our analysis of surveys and in-depth interviews collected with DACA recipients in California provides context for this finding. DACA enabled college for some, but discouraged it for others. DACA recipients perceived substantial occupational mobility, but this was not reflected in movement out of the secondary labor market for many. Our findings suggest that without access to permanent legal status, DACA recipients will experience liminal legality with limited and contingent impacts on socioeconomic integration.
The Interplay Between Emotional Intelligence, Tramadol Dependence, and Comorbid Personality Disorder
Authors: Nagy Abdelhamid Nahla E. , Mohsen Ibrahim Nesreen M. , Hashem Reem E.M. , Marzouk Sarah A.I. , Abd El Moneam Mohamed H. 
Published: March 2020

Objectives:
Various studies have proven the presence of disturbances in emotional processing among patients with substance abuse. In the current study, we aimed to examine the relationship between emotional intelligence (EI), tramadol dependence, and comorbid personality disorders.

Methods:
Thirty adult male patients with tramadol dependence and 30 healthy controls were enrolled in the current study. Participants were assessed by the Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition Axis I diagnosis (SCID-I) and the Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition Axis-II Personality Disorders (SCID-II). EI was assessed using the Schutte Self-Report Emotional Intelligence Test.

Results:
With respect to the Schutte Self-Report Emotional Intelligence Test score, there was a statistically significant difference between patients with tramadol dependence and the control group in all domains of EI (P<0.001). With respect to the SCID-II, the most common personality disorders reported among patients with tramadol dependence were borderline personality disorder (13, 43.3%), followed by depressive personality disorder (12, 40%) and narcissistic personality disorder (10, 33.3%). There was no statistically significant difference between common personality disorders and all domains of EI.

Conclusion:
The susceptibility to tramadol dependence is most likely to underlie disturbances in EI, suggesting that treatment strategies targeting the management of emotions would reduce this risk.
Isoflurane Sedation in Patients Undergoing Venoarterial Extracorporeal Membrane Oxygenation Treatment for Cardiogenic Shock—An Observational Propensity-Matched Study
Authors: Scherer Clemens , Kupka Danny , Stocker Thomas J , Joskowiak Dominik , Scheuplein Hanna , Schönegger Carmen Maria , Born Frank , Stremmel Christopher , Lüsebrink Enzo , Stark Konstantin , Orban Mathias , Petzold Tobias , Peterss Sven , Hausleiter Jörg , Hagl Christian , Massberg Steffen , Orban Martin 
Published: March 2020

Objectives:
The feasibility and hemodynamic effects of isoflurane sedation in cardiogenic shock in the presence of venoarterial extracorporeal membrane oxygenation treatment are currently unknown.

Design:
Retrospective single-center study.

Setting:
Cardiac ICU of Munich university hospital.

Patients/Subjects:
Cardiogenic shock patients with venoarterial extracorporeal membrane oxygenation treatment under sedation with volatile isoflurane between November 2018 and October 2019 have been enrolled in this study and were matched by propensity score in a 1:1 ratio with IV sedated patients treated between January 2013 and November 2018 from the cardiogenic shock registry of the university hospital of Munich.

Measurements and Main Results:
Isoflurane sedation was used in 32 patients with cardiogenic shock and venoarterial extracorporeal membrane oxygenation treatment. The mean age of conventionally sedated patients was 58.4 ± 13.8 years and 56.3 ± 11.5 years for patients with isoflurane sedation (p = 0.51). Administration of isoflurane was associated with lower IV sedative drug use during venoarterial extracorporeal membrane oxygenation treatment (86% vs 32%; p = 0.01). Mean systolic arterial pressure was similar (94.3 ± 12.6 vs 92.9 ± 10.5 mm Hg; p = 0.65), but mean heart rate was significantly higher in the conventional sedation group, when compared with the isoflurane group (85.2 ± 20.5 vs 74.7 ± 15.0 beats/min; p = 0.02). Catecholamine doses, venoarterial extracorporeal membrane oxygenation blood and gas flow, ventilation time (304 ± 143 vs 398 ± 272 hr; p = 0.16), bleeding complications bleeding academic research consortium 3a or higher (59.3% vs 65.3%; p = 0.76), and 30-day mortality (59.2% vs 63.4%, p = 0.80) were similar in both groups. The overall sedation costs per patient were significantly lower in the conventional group, when compared with the isoflurane group (537 ± 624 vs 1280 ± 837 €; p < 0.001).

Conclusions:
Volatile sedation with isoflurane is feasible—albeit at higher costs—in patients with cardiogenic shock and venoarterial extracorporeal membrane oxygenation treatment and was not associated with higher catecholamine dosage or extracorporeal membrane oxygenation flow rate compared with IV sedation.
Secretin-Stimulated Magnetic Resonance Imaging Reveals Variable Diagnostic Accuracy According to Etiology in Pancreatic Disease
Authors: Engjom Trond , Tjora Erling , Erchinger Friedemann , Madzak Adnan , Dimcevski Georg , Frøkjær Jens Brøndum , Haldorsen Ingfrid Salvesen 
Published: March 2020

Objectives
Secretin-stimulated magnetic resonance imaging (s-MRI) is the best validated radiological modality assessing pancreatic exocrine secretion. In this prospective observational study, we compare the diagnostic accuracy of s-MRI for exocrine pancreatic failure due to different pancreatic diseases and healthy controls.

Methods
We performed s-MRI in 21 cystic fibrosis (CF) patients, 78 patients with chronic pancreatitis (CP) and 20 healthy controls. Exocrine failure was defined by fecal elastase-1 of less than 200 μg/g or bicarbonate concentration from endoscopic secretin test of less than 80 mmol/L.

Results
Eleven CF and 61 CP patients were exocrine insufficient. Insufficient CF patients had lower s-MRI volume output compared with all other groups (P < 0.05). Insufficient CP patients had reduced volume output compared with controls and sufficient CF (P < 0.05). Secretin-stimulated MRI yielded overall accuracy of 0.78 (95% confidence interval [CI], 0.70–0.86) for exocrine failure. When divided according to etiology, the test yielded accuracy of 0.95 (95% CI, 0.90–1) in CF and 0.73 (95% CI, 0.64–0.82) in CP.

Conclusions
The accuracy of s-MRI volume output measures to diagnose exocrine failure was higher in CF than in CP. Differences in s-MRI volume output in patients with exocrine failure may be due to different etiological and pathogenic mechanisms in CF and CP.
Radiographic and Endoscopic Features of Pancreaticoduodenal Malakoplakia
Authors: Hubbard Lauren , Iriana Sentia , Carey Adrienne , Odrobina Robert , Sossenheimer Michael , Rogers Douglas 
Published: March 2020

AbstractMalakoplakia is a rare, granulomatous disorder that is typically triggered by infections in immunocompromised patients. Although it most commonly affects the urinary tract, cases may occasionally occur in the gastrointestinal tract. There are case reports of malakoplakia of the pancreas with associated pathologic description, but none with detailed imaging and endoscopic findings. In addition, description of magnetic resonance imaging characteristics of mass-forming malakoplakia in the literature is sparse. We present a case of pancreaticoduodenal malakoplakia in an immunocompromised patient, including detailed description of magnetic resonance imaging, computed tomography, and endoscopic findings with radiology-pathology correlation. Classic pathologic features of malakoplakia (eg, hypercellularity, inflammation, and mineralization of Michaelis-Gutmann bodies) lead to specific features on imaging, such as marked diffusion restriction, heterogeneous enhancement, calcification, and increased attenuation on nonenhanced computed tomography. These features may help differentiate malakoplakia from other more common lesions that occur in this location, especially if present in an immunocompromised patient.
Prediction of Pancreatic Cancer Based on Imaging Features in Patients With Duct Abnormalities
Authors: Chen Wansu , Butler Rebecca K. , Zhou Yichen , Parker Rex A. , Jeon Christie Y. , Wu Bechien U. 
Published: March 2020

Objectives
Abnormalities of the main pancreatic duct may be an early indicator of pancreatic ductal adenocarcinoma (PDAC). We develop and validate algorithms that predict the risk of PDAC using features identified on cross-sectional imaging and other clinical characteristics collected through electronic medical records.

Methods
Adult patients with abdominal computed tomography or magnetic resonance imaging in January 2006 to June 2016 demonstrating dilatation of main pancreatic duct were identified. Pancreas-related morphologic features were extracted from radiology reports using natural language processing. The cumulative incidence of PDAC with death as a competing risk was estimated using multistate models. Model discrimination was assessed using c-index. The models were internally validated using bootstrapping.

Results
The cohort consisted of 7819 patients (mean age, 71 years; 65% female). A total of 781 patients (10%) developed PDAC within 3 years after the first eligible imaging study. The final models achieved reasonable discrimination (c-index, 0.825–0.833). The 3-year average risk of PDAC in the top 5% of the total eligible patients was 56.0%, more than 20 times of the average risk among the bottom 50% of patients.

Conclusions
Prediction models combining imaging features and clinical measures can be used to further stratify the risk of pancreatic cancer among patients with pancreas ductal dilatation.
Tenascin C in the Tumor-Nerve Microenvironment Enhances Perineural Invasion and Correlates With Locoregional Recurrence in Pancreatic Ductal Adenocarcinoma
Authors: Furuhashi Satoru , Sakaguchi Takanori , Murakami Tomohiro , Fukushima Mayu , Morita Yoshifumi , Ikegami Koji , Kikuchi Hirotoshi , Setou Mitsutoshi , Takeuchi Hiroya 
Published: March 2020

Objectives
Perineural invasion is common in pancreatic ductal adenocarcinoma (PDAC) and worsens the postoperative prognosis. Tenascin C (TNC), an extracellular matrix glycoprotein, modulates tumor progression. We evaluated the functional roles of TNC, especially in perineural invasion of PDAC.

Methods
We examined immunohistochemical TNC expression in 78 resected PDAC specimens. The relationships between TNC expression and clinicopathological features were retrospectively analyzed. Interactions between cancer cells and nerves with TNC supplementation were investigated using an in vitro coculture model with PDAC cell line and mouse dorsal root ganglion (DRG).

Results
Tenascin C expression was predominant in perineural sites at the invasive tumor front. High perineural TNC expression in 30 patients (38%) was associated with perineural invasion, pathological T stage ≥3, and postoperative locoregional recurrence. High TNC expression was independently associated with postoperative, poor recurrence-free survival by multivariate analysis. In the in vitro coculture model, a TNC-rich matrix enhanced both PDAC cell colony extensions toward nerves and DRG axonal outgrowth toward cancer cell colonies, whereas TNC did not affect axonal outgrowth or cancer cell proliferation in separately cultured DRG and PDAC cells.

Conclusions
Strong perineural TNC expression indicated poor prognosis with locoregional recurrence. The neurotropism of TNC-induced PDAC suggests that TNC is a potential PDAC therapeutic target.
Clinical Characteristics on 25 Discharged Patients with COVID-19 Virus Returning
Authors: Yuan Jing , Kou Shanglong , Liang Yanhua , Zeng JianFeng , Pan Yanchao , Liu Lei 
Published: March 2020

AbstractHere we report the clinical features of 25 discharged patients with COVID-19 recovery. Our analysis indicated that there was a significant inverse correlation existed between serum D-Dimer level and the duration of antiviral treatment, while lymphocyte concentration significantly positively correlated with the duration of virus reversal.
Clinical outcomes of 402 patients with COVID-2019 from a single center in Wuhan, China
Authors: Wu Yingjie , Guo Wei , Liu Huan , Qi Bei , Liang Ke , Xu Haibo , Peng Zhiyong , Xiao Shu-Yuan 
Published: March 2020

AbstractThe SARS-CoV-2 outbreak is causing widespread infections and significant mortality. Previous studies describing clinical characteristics of the disease contained small cohorts from individual centers or larger series consisting of mixed cases from different hospitals. We report analyses of mortality and disease severity among 402 patients from a single hospital. The cohort included 297 patients with confirmed and 105 with suspected diagnosis. The latter group met the criteria for clinical diagnosis but nucleic acid tests results were initially interpreted as suspicious. Data were compared between genders and among different age groups. The overall case fatality is 5.2%. However, patients 70 years of age or older suffered a significantly higher mortality (17.8%), associated with more patients having severe or critical illness (57.5%). Patients 50 years of age or older had a mortality of 8.0%, and those younger than 50 years, 1.2%. Male patients had a mortality of 7.6% versus 2.9% in females.
Screening of Potential Biomarkers for Gastric Cancer with Diagnostic Value Using Label-free Global Proteome Analysis
Authors: Song Yongxi , Wang Jun , Sun Jingxu , Chen Xiaowan , Shi Jinxin , Wu Zhonghua , Yu Dehao , Zhang Fei , Wang Zhenning 
Published: March 2020

AbstractGastric cancer (GC) is one of the most malignant tumors worldwide. Despite the recent decrease in mortality rates, the prognosis remains poor. Therefore, it is necessary to find novel biomarkers with early diagnostic value for GC. In this study, we present a large-scale proteomic analysis of 30 GC tissues and 30 matched healthy tissues using label-free global proteome profiling. Our results identified 537 differentially expressed proteins, including 280 upregulated and 257 downregulated proteins. The ingenuity pathway analysis (IPA) results indicated that the sirtuin signaling pathway was the most activated pathway in GC tissues whereas oxidative phosphorylation was the most inhibited. Moreover, the most activated molecular function was cellular movement, including tissue invasion by tumor cell lines. Based on IPA results, 15 hub proteins were screened. Using the receiver operating characteristic curve, most of hub proteins showed a high diagnostic power in distinguishing between tumors and healthy controls. A four-protein (ATP5B-ATP5O-NDUFB4-NDUFB8) diagnostic signature was built using a random forest model. The area under the curve (AUC) of this model was 0.996 and 0.886 for the training and testing set, respectively, suggesting that the four-protein signature has a high diagnostic power. This signature was further tested with independent datasets using plasma enzyme-linked immune sorbent assays, resulted in an AUC of 0.778 for distinguishing GC tissues from healthy controls, and using immunohistochemical tissue microarray analysis, resulting in an AUC of 0.805. In conclusion, this study identifies potential biomarkers and improves our understanding of the pathogenesis, providing novel therapeutic targets for GC.
Outcomes following short hospital stay after total knee replacement in a regional setting: A prospective analysis of an observational cohort in a public hospital treated 2018 - 2019
Authors: Fatima Manaal , Scholes Corey , Tutty Amanda , Ebrahimi Milad , Genon Michel , Martin Samuel J. 
Published: March 2020

AbstractFunctional outcomes and patient satisfaction following short length of stay (LoS) after total knee arthroplasty (TKA) in the regional context have not been explored. This study aimed to report on complications, functional outcomes and satisfaction of patients discharged from acute (≤2 days) stay, up to 6 weeks after TKA in a regional hospital.Patients were prospectively recruited from August 2018 to August 2019. Demographic data, intraoperative factors and the incidence of complications and emergency department (ED) presentations were retrieved from hospital records. Preoperative and 6 weeks postoperative assessments collected range of motion (ROM), timed up and go (TUG), 6 minute walk test (6MWT) and total Oxford knee score (OKS), with patient satisfaction assessed at follow-up via a questionnaire. A directed acyclic graph approach was used to perform logistic and linear regression to assess relationships between patient and treatment characteristics with short-term outcomes.Median LoS for the period was 2 days, influenced by age, gender, bilateral status and body mass index. A shorter LoS was significantly associated with functional outcomes and patient satisfaction, with 74.4% of patients satisfied with their knee and 88.4% satisfied with their LoS. At six weeks, significant improvements in all scores were found, however only the change in OKS exceeded the minimal clinically important difference (MCID) threshold. Patient satisfaction correlated with change in TUG exceeding MCID. Complications at 6 weeks post surgery were affected by Aboriginal and Torres Strait Islander status, marriage status, smoking history, history of chronic pain and mental health conditions, and the number of ED presentations was associated with preoperative TUG and comorbidities.The findings establish that short LoS after TKA in a regional hospital is associated with good outcomes and a high patient satisfaction, but patient factors and comorbidities associated with an increased risk of complications and ED presentations should be considered for postoperative management and patient care.
TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control
Authors: Douse Christopher H. , Tchasovnikarova Iva A. , Timms Richard T. , Protasio Anna V. , Seczynska Marta , Prigozhin Daniil M. , Albecka Anna , Wagstaff Jane , Williamson James C. , Freund Stefan M.V. , Lehner Paul J. , Modis Yorgo 
Published: March 2020

SummaryThe Human Silencing Hub (HUSH) complex epigenetically represses retroviruses, transposons and genes in vertebrates. HUSH therefore maintains genome integrity and is central in the interplay between intrinsic immunity, transposable elements and transcriptional regulation. Comprising three subunits – TASOR, MPP8 and Periphilin – HUSH regulates SETDB1-dependent deposition of the transcriptionally repressive epigenetic mark H3K9me3 and recruits MORC2 to modify local chromatin structure. However the mechanistic roles of each HUSH subunit remain undetermined. Here we show that TASOR lies at the heart of HUSH, providing a platform for assembling the other subunits. Targeted epigenomic profiling supports the model that TASOR binds and regulates H3K9me3 specifically over LINE-1 repeats and other repetitive exons in transcribed genes. We find TASOR associates with several components of the nuclear RNA processing machinery and its modular domain architecture bears striking similarities to that of Chp1, the central component of the yeast RNA-induced transcriptional silencing (RITS) complex. Together these observations suggest that an RNA intermediate may be important for HUSH activity. We identify the TASOR domains necessary for HUSH assembly and transgene repression. Structural and genomic analyses reveal that TASOR contains a poly-ADP ribose polymerase (PARP) domain dispensable for assembly and chromatin localization, but critical for epigenetic regulation of target elements. This domain contains a degenerated and obstructed active site and has hence lost catalytic activity. Together our data demonstrate that TASOR is a pseudo-PARP critical for HUSH complex assembly and H3K9me3 deposition over its genomic targets.
Detailed analysis of public RNAseq data and long non-coding RNA: a proposed enhancement to mesenchymal stem cell characterisation
Authors: Riquier Sebastien , Mathieu Marc , Boureux Anthony , Ruffle Florence , Lemaitre Jean-Marc , Djouad Farida , Gilbert Nicolas , Commes Therese 
Published: March 2020

AbstractThe development of RNA sequencing (RNAseq) and corresponding emergence of public datasets have created new avenues of transcriptional marker search. The long non-coding RNAs (lncRNAs) constitute an emerging class of transcripts with a potential for high tissue specificity and function. Using a dedicated bioinformatics pipeline, we propose to construct a cell-specific catalogue of unannotated lncRNAs and to identify the strongest cell markers. This pipeline usesab initiotranscript identification, pseudoalignment and new methodologies such as a specific k-mer approach for naive quantification of expression in numerous RNAseq data.For an application model, we focused on Mesenchymal Stem Cells (MSCs), a type of adult multipotent stem-cells of diverse tissue origins. Frequently used in clinics, these cells lack extensive characterisation. Our pipeline was able to highlight different lncRNAs with high specificity for MSCs.In silicomethodologies for functional prediction demonstrated that each candidate represents one specific state of MSCs biology. Together, these results suggest an approach that can be employed to harness lncRNA as cell marker, showing different candidates as potential actors in MSCs biology, while suggesting promising directions for future experimental investigations.