bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reinvestigation of the origins of pineal meningiomas based on its related veins and arachnoid membranes
Authors: Yu Lei , Orazmyradov Berdimyrat , Qi Songtao , Song Ye , Fang Luxiong 
Published: May 2020

Abstract
Background: A series of patients harboring pineal region meningiomas were respectively analyzed to explore the origin of these tumors and the true meaning of the term "velum interpositum (VI) meningiomas".Methods: 21 patients with pineal meningiomas underwent operation in Nanfang Hospital of Southern Medical University from January 2005 to December 2016 were retrospectively included to analyze the clinical features, imaging findings and surgical video data of these patients. According to the method of literature, the data of this group were also divided into falcotentorial (FT) meningiomas and VI meningiomas, and the differences between the two types of tumors were compared.Results: Among the 21 cases of tumor, there were 12 cases of FT meningiomas, including 4 cases originating from cerebral falx, 4 cases from tentorium of cerebellum and 4 cases from straight sinus; there were 9 cases of VI meningiomas, 7 of which originated from the arachnoid sleeve of the Galen vein, 1 from the posterior part of the internal cerebral vein and 1 from the posterior surface of the pineal gland. Postoperative pathological examination showed meningiomas in all the 21 patients, including 16 cases of total resection and 5 cases of subtotal resection. Postoperatively limitation of binocular vertical motion was found in 3 cases, homotropic hemianopia in 7 cases, hemiplegia in 1 case and death in 1 case.Conclusions: This study suggests that pineal meningiomas are more suitable to be described by FT meningioma and meningiomas of the arachnoid of the pineal region by analyzing the origin of tumors. The term "VI meningiomas" can only reflect a part of meningiomas of the arachnoid of the pineal region. Before the removal of pineal meningiomas, more attention should be paid to the effects of the two types of tumors on the Galen vein and the straight sinus, and the establishment of venous collateral circulation.
Glycinin-induced porcine IPEC-J2 cells damage via the NF-κB/MAPK signaling pathway
Authors: Peng Chenglu , Sun Zhifeng , Wang Lei , Shu Yingshuang , He Mengchu , Ding Hongyan , Li Yu , Wang Xichun , Feng Shibin , Li Jinchun , Wu Jinjie 
Published: May 2020

Abstract
Background: Glycinin, a protein found in soybean, is a human and animal allergen that causes damage to the intestinal barrier. However, its mechanisms of action remain unclear. Therefore, in this study, the intestinal porcine epithelial cell line IPEC-J2 was used to evaluate the effect of glycinin concentration on the intestinal epithelium and identify the related signaling pathways. Results: IPEC-J2 cells were divided into seven treatment groups and a control group; the cells were treated for 24 h with 1, 5, or 10 mg/mL glycinin or with 5 mg/mL glycinin after 30 min of pre-treatment with 1 μmol/L nuclear factor-kappa B (NF-κB) inhibitor (pyrrolidine dithiocarbamate), inducible nitric oxide synthase inhibitor ( N -ω-nitro-l-arginine methyl ester), Jun N-terminal kinase (JNK) inhibitor (SP600125), or p38 inhibitor (SB202190). A series of molecular and biochemical experiments revealed that the levels of NF-κB, p38, and JNK, as well as their downstream proteins, were increased after treatment compared to those in the control group. Conclusion: Glycinin damaged IPEC-J2 cells in a concentration-dependent manner via the NF-κB/MAPK signaling pathway.
Closed-loop target-controlled infusion system for propofol guided by bispectral index applied in patients undergoing shoulder arthroscopy in the beach chair position- a randomized controlled trial
Authors: Zhao Zhao , Li Junjie , Fang Yang , Nanbo Luo , Han Yang , Xinping Yang , Liangquan Peng , Wei Lu , Fang Guangguang , Daping Wang , Liu Zhiheng 
Published: May 2020

Abstract
Background To compare the efficacy of anesthetic depth control using closed-loop and open-loop target controlled infusion (TCI) system of propofol guided by BIS in patients undergoing shoulder arthroscopy in the beach chair position (BCP). Methods 120 patients underwent shoulder arthroscopy surgery in the BCP were randomized into two groups, the open-loop (O) group and the closed-loop (C) group. During the maintenance phase, BIS value was used as the feedback variable for TCI system of propofol in both groups. The Global score (GS) and the percentage of adequate anesthesia, the frequency of propofol regulation, and consumption of propofol were calculated. The MMSE scores of the day before and 1 day after surgery, serum GFAP and S100B proteins before anesthesia, after extubation and 1 day after surgery were compared. Results The GS and the proportion of appropriate anesthesia time were better in the group C. The percentage of overshoot time was lower in the group C. The frequency of propofol regulation was observed higher in the group C. Propofol consumption in the group C was significantly lower than that in the group O. The MMSE scores, the GFAP and S100B protein concentrations had no significant difference between the two groups. Conclusion Propofol administration using close-loop TCI system guided by BIS may increase the percentage of adequate anesthesia and shorten the percentage of overshoot time compared with open-loop TCI model in anesthesia maintenance phase in patients undergoing shoulder arthroscopy in the BCP, and do not increase the risk of POCD.
Trial staff views on barriers recruitment in a digital intervention for psychosis and how to work around them: A qualitative study within a trial
Authors: Allan Stephanie , Mcloed Hamish , Bradstreet Simon , Morton Emma , Bell Imogen , Wilson-Kay Alison , Whitehill Helen , Clark Andrea , Matrunola Claire , Farhall John , Gleeson John , Gumley Andrew 
Published: May 2020

Abstract
Background: Recruitment processes for clinical trials of digital interventions for psychosis are seldom described in detail within the literature. While trial staff have expertise in describing barriers and facilitators to recruitment a specific focus on understanding recruitment from the point of view of trial staff is rare.Methods: We applied pluralistic ethnographic methods including analysis of trial documents, observation and focus groups explored the recruitment processes of the EMPOWER feasibility trial (ISRCTN: 99559262).Results: Recruitment barriers fell into two main themes; service characteristics (lack of time available to mental health staff to support recruitment, staff turnover, patient turnover (within Australia only), management styles of community mental health teams, physical environment) and clinician expectations (filtering effects and resistance to research participation). Trial staff negotiated these barriers through strategies such as emotional labour (trial staff managing feelings and expressions in order to successfully recruit participants) and trying to build relationships with clinical staff working within community mental health teams.Conclusions: Researchers in clinical trials for digital psychosis interventions face numerous recruitment barriers and do their best to work flexibly negotiate these barriers and meet recruitment targets. The recruitment process appeared to be enhanced by trial staff supporting each other throughout the recruitment stage of the trial.Trial Registration: (ISRCTN: 99559262 registered 21/12/2015)
MiR-199b-3p prevent the epithelial-mesenchymal transition (EMT) and fibrosis of renal tubule by regulating E-cadherin through targeting KDM6A in Diabetic nephropathy (DN)
Authors: Tang Bo , Li Weiliang , Ji Tingting , Li Xiaoying , Qu Xiaolei , Bai Shoujun 
Published: May 2020

Abstract
Background

Diabetic nephropathy (DN) is the leading cause of end-stage renal disease. Although dysfunction of renal tubule, also exhibited as epithelial-mesenchymal transition (EMT) and fibrosis, is closely associated with DN, the mechanism underlying renal tubule dysfunction still remains obscure.
Methods

Here, we identify that miR-199b-3p protect renal tubule from diabetic- induced injury by repressing KDM6A, a histone lysine demethylase reinforcing diabetic renal tubule dysfunction through regulating E-cadherin expression. We investigated the relationship between KDM6A, E-cadherin and miR-199b-3p with a series of gain- and loss- function assay in different cell models and animal models. The expression of KDM6A, E-cadherin and miR-199b-3p was tested by qPCR, western bolt or IHC. The EMT was measured by wound healing assay. The dysfunction of renal tubule were observed through HE and PAS stain and the kidney functions were monitored through several pathological signs detection assay, such as albumin to creatinine ratio (ACR), blood urea nitrogen (BUN), creatinine (Cr), proteinuria.
Results

Lower expression of E-cadherin was related to a higher level of KDM6A, while E-cadherin was increased with KDM6A-inhibitor GSK-J4 treatment both in HG-induced HK-2 cells and STZ-induced kidney. However, the variable expression of E-cadherin caused by overexpression or silence RNA was failed to alter the KDM6A expression. The target prediction and dual-luciferase assay results showed miR-199b-3p is a new miRNA targeting KDM6A. Overexpression of miR-199b-3p increased E-cadherin expression and prevented EMT through repressing KDM6A expression in HG-induced HK-2 cells, whereas miR-199b-3p knockdown by inhibitor displayed opposite results with lower E-cadherin and worse EMT accompanying with higher level of KDM6A. In addition, the miR-199b-3p knockout mice exhibited more dysfunctional renal tubule and more serious damage in kidney tissue treated with STZ.
Conclusions

These results demonstrate that miR-199b-3p improve E-cadherin expression and prevent the progression of DN through targeting KDM6A. MiR-199b-3p could be a potential biomarker or target for the diagnosis and treatment of diabetic nephropathy in the future.
LINC01255 combined with BMI1 to regulate Human Mesenchymal Stromal Senescence and Acute myeloid leukemia cell proliferation through repress the transcription of MCP-1
Authors: Liu Qinghua , Dong Jing , Li Jie , Duan Yanchao , Wang Keqiang , Kong Qiuhong , Zhang Hong 
Published: May 2020

Abstract
Background: Long non-coding RNAs (lncRNAs) govern fundamental biochemical and cellular biology processes, for example, participate in chromatin remodeling, imprinting, splicing, transcriptional regulation and translation. Dysregulation of lncRNA expression is act as a feature of various diseases and cancers, including hematopoietic malignancies. However, the clinical relevance of myelodysplastic syndrome (MDS) and acute myeloid leukemia preceded by MDS (MDS-AML) requires further research. Recently, lncRNAs have been demonstrated plays an important role in hematopoiesis, thus, to further finding more functional lncRNA seemed particularly important.Methods: Western blotting, real-time PCR, RNA-pulldown, RIP, Chromatin immunoprecipitation (ChIP), cellular compartments extraction assays, SA-β-gal staining, lentivirus transfection, cell viability assay and cell proliferation assays were used to examine the relationship between lncRNA LINC01255 and its regulation of p53-p21 pathway in human mesenchymal stromal and acute myeloid leukemia cells.Results: LncRNA LINC01255 is highly expressed in bone marrow cells of AML patients, CD34+ cells of MDS-AML patients and AML cell lines and the higher expression of LINC01255 is assocoated with poor survival rate of AML patients. LINC01255 can interact with BMI1 and repress the transcription of MCP-1 to active p53-p21 pathway, thus inhibiting the senescence of human mesenchymal stromal and proliferation of acute myeloid leukemia cell.Conclusions: We discovered a novel functional lncRNA LINC01255, which can regulate the senescence of human mesenchymal stromal and the proliferation of acute myeloid leukemia cell through inhibiting the transcription of MCP-1.
Age associated level of estradiol (E2) and progesterone (4) influence IL-8 response to Mycobacterium tuberculosis (Mtb) antigens in women.
Authors: MENSAH Priscillia Virginie Liesse MBOUROU , LEBOUENY Marielle , ESSONE Paulin NDONG , SIAWAYA Anicet Christel MALOUPAZOA , ALAME-EMANE Amel Kévin , BIYOGO Oriane Cordelia ABOUMEGONE , TADATSIN Patrice Hemery DAPNET , NZOGHE Amandine MVEANG , ESSAMAZOKOU Dimitri Ulrich , NDJINDJI Ofilia MVOUNDZA , PADZYS Guy-Stéphane , ONDO Joseph Privat , TAKIFF Howard , GICQUEL Brigitte , Siawaya Joel Fleury Djoba 
Published: May 2020

Abstract
Tuberculosis (TB) is an intracellular infection controlled the effective recruitment of effectors immune cells at infection site. In aged premenopausal or menopausal women, there is an increased pro-inflammatory cytokines secretion suggesting an underlying link between cytokines response and estrogen (E2) and progesterone (P4) levels. In this study we compared women aged 40 years old and above (premenopausal) and women aged below 40 years old with and without latent TB infection to determine the link between E2, P4 and cytokines response to Mycobacterium tuberculosis (M. tuberculosis) stimuli. E2 and P4 levels were significantly higher in women under 40 years old than in women above 40 years old irrespective their LTB status (p < [0.0001–0.05]). In women under 40 years old, E2 and P4 were found to correlate negatively and significantly with IL-8 response to M. tuberculosis antigens stimulation ((p < [0.001–0.01]). Furthermore, M. tuberculosis IL-8 specific response was significantly higher in women above 40 years old than women under 40 years old. This study demonstrates that women aging and the linked hormonal changes are associated with hence IL-8 response to M. tuberculosis antigen, which may have implications for the age-related susceptibility or resistance to active tuberculosis.
Trends in the outpatient-visits and allergens of allergic rhinitis in Chongqing, China from 2008 to 2017
Authors: shen yang , Zou Qi-Yuan , Li Jia , Ke Xia , Kang Hou-Yong , Hong Su-Ling 
Published: May 2020

Abstract
Background The prevalence of allergic rhinitis (AR) has increased globally. This study was conducted to investigate the changing trends of AR’ s outpatient visits and inhaled allergens, also the possible association between air pollution and AR visits in Chongqing, China.Methods We analyzed all the skin prick test results from patients diagnosed as AR in Departments of Otolaryngology of the first affiliated hospital of Chongqing Medical University during 2008 to 2017. The trend of AR visit rate and inhaled allergens was observed. Then, air quality data were acquired from the Chongqing Municipal Environmental Protection Monitoring Center. We explored the effects of air pollutants (SO2, NO2, PM2.5, PM10, CO and O3) on outpatient visits for AR.Results From 2008 to 2017, the ratio of AR outpatient visits increased. The number of polysensitization was more than that of monosensitization. However, the rate of mono-specific sensitization to dust mite allergen was on the increase, especially in patients of 20–39 y. No correlations were found between AR visits and pollutants, while mono-mite-sensitized AR visits were significantly correlated with the concentrations of atmospheric SO2, PM10 and PM2.5.Conclusions the rising trends in AR visits reflect both a real increase in the occurrence and a more effective diagnosis of cases. Increment in mono-mite sensitization implies the rising trends of new AR cases. The significant correlation between mono-mite-sensitized AR visits and concentration of SO2, PM10 and PM2.5 supports opinion about an influence of atmospheric pollutants on mite induced immune response in AR.
Short bouts of gait data and inertial sensors can provide reliable measures of spatiotemporal gait parameters from bilateral gait data of participants with multiple sclerosis
Authors: Ader Lilian Genaro Motti , Greene Barry R. , McManus Killian , Tubridy Niall , Caulfield Brian 
Published: May 2020

Abstract
Background:Wearable devices equipped with inertial sensors enable objective gait assessment for persons with multiple sclerosis (MS), with potential use in ambulatory care or home and community-based assessments. However, gait data collected in non-controlled settings is often fragmented and may not provide enough information for reliable measures. We evaluate a novel approach, extracting pre-defined numbers of gait cycles from the full length of a walking task, and their effects on the reliability of spatiotemporal gait parameters.Methods:The present study evaluates intra-session reliability of spatiotemporal gait parameters for short bouts of gait data extracted from the full length of the walking tasks to 1) determine the effects of the length of the walking task on the reliability of calculated measures and 2) identify spatiotemporal gait parameters that can provide reliable measures for gait assessments and reference data in different settings.Thirty-seven participants (37) diagnosed with relapsing-remitting MS (EDSS rage 0 to 4.5) executed two trials, walking 20m each, with inertial sensors attached to their right and left shanks. Previously published algorithms were applied to identify gait events from the medio-lateral angular velocity. Short bouts of gait data were extracted from each trial, with lengths varying from 3 to 9 gait cycles. Twenty-one measures of spatiotemporal gait parameters were calculated. Intraclass correlation coefficients (ICCs) were calculated to evaluate how the degree of agreement between the two trials of each participant varied with the number of gait cycles included in the analysis.Results:Spatiotemporal gait parameters calculated as the mean across included gait cycles reach excellent reliability from three gait cycles. Stride time variability and asymmetry, as well as stride velocity variability and asymmetry, reach good reliability from six gait cycles and should be further explored for persons with MS, while stride time asymmetry and step time asymmetry do not seem to provide reliable measures and should be reported carefully.Conclusion:Short bouts of gait data, including at least six gait cycles of bilateral data, can provide reliable gait measurements for persons with MS, opening new perspectives for gait assessment using wearable devices in non-controlled environments, to support monitoring of symptoms of persons with neurological diseases.Trial registrationNot applicable.
A Cohort Study Evaluating the Association Between Concurrent Mental Disorders, Mortality, Morbidity, and Continuous Treatment Retention for Patients in Opioid Agonist Treatment (OAT) Across Ontario, Canada Using Administrative Health Data
Authors: Morin Kristen , Eibl Joseph K , Gauthier Graham , Rush Brian , Mushquash Christopher , Lightfoot Nancy E , Marsh David 
Published: May 2020

Abstract
Background: Due to the high prevalence of mental disorders among people with opioid use disorder, the objective of this study was to determine the association between concurrent mental disorders, mortality, morbidity and continuous treatment retention for patients in opioid agonist treatment in Ontario, Canada. Methods: We conducted a retrospective cohort study of patients enrolled in opioid agonist treatment between January 1, 2011, and December 31, 2015. Patients were stratified into two groups: those diagnosed with concurrent mental disorders and opioid use disorder and those with opioid use disorder only, using data from the Ontario Health Insurance Plan Database, Ontario Drug Benefit Plan Database. The primary outcome studied was all-cause mortality using data from the Registered Persons Database. Emergency Department visits from the National Ambulatory Care Database, hospitalizations Discharge Abstract Database, and continuous retention in treatment, defined as one year of uninterrupted opioid agonist treatment using data from the Ontario Drug Benefit Plan Database, were measured as secondary outcomes. Encrypted patient identifiers were used to link information across databases.Results: We identified 55,924 individuals enrolled in opioid agonist treatment, and 87% had a concurrent mental disorder diagnosis during this period. We observed that having a mental disorder was associated with an increased likelihood of all-cause mortality (Odds Ratio (OR) 1.4; 95% Confidence Interval (CI) 1.2-1.5, frequent emergency department visits (OR 3.69; 95% CI 3.7-4.1) and hospitalizations (OR 2.6; 95%CI 2.5-2.7). However,there was no association between having a diagnosis of a mental disorderand one-year treatment retention in OAT OR 1.0; 95%CI 0.9-1.1). Conclusion: Our findings highlight the consequences of the high prevalence of mental disorders for individuals with opioid use disorder in Ontario, Canada
Inhibition Mechanism of Caffeine in Tea Pathogenic Fungi Botryosphaeria Dothidea and Colletotrichum Gloeosporioides
Authors: Thangaraj Kuberan , Deng Cheng , Cheng Lin-Lin , Deng Wei-Wei , Zhang Zheng-Zhu 
Published: May 2020

Abstract
Background Caffeine acts as an antifungal agent; however, its underlying inhibition mechanism remains unclear. To investigate variations in caffeine antifungal activity, this study was evaluate the inhibition mechanisms of caffeine on phytopathogens Botryosphaeria dothidea and Colletotrichum gloeosporioides through transcriptomic analysis, which causes leaf necrosis and anthracnose on tea leaves, respectively.Results Our in vitro results shows that the antifungal activity of caffeine quite different between B. dothidea and C. gloeosporioides. Furthermore, microscopic observation and bioactivity assay results proved that caffeine destroyed the fungal protective layers (cell wall and cell membrane) and subcellular organelles. The transcriptomes of these two fungi exposed to different caffeine concentrations were analyzed by high-throughput sequencing and the results showed that caffeine repressed gene expression involved in the pathways of mRNA surveillance, ribosome biogenesis in eukaryotes, aminoacyl-tRNA biosynthesis, etc. Particularly, caffeine affected gene expression in melanin biosynthesis in C. gloeosporioides, resulting in the disturbances of melanin production and fungal pathogenicity. Manually infected tea leaves showed melanin pigment was important to appressorium development and fungal infection by histopathological observation and fungal biomass quantification. Besides, C. gloeosporioides successfully enters into the host tissue even plant could build multilayer defenses against the fungus, this might be due to the variation in pathogen targets and quantities of secondary metabolites by the host cells.Conclusions Overall, our findings indicate that caffeine in the environment can reduce the fungal growth and physiological activities. However, the genome-wide expression showed different transcriptional profiles between these fungal isolates. Thus, we believe that target of the caffeine varied among isolates and melanin may play a major role in fungus caffeine tolerance. Further in-depth research required to conclude this hypothesis.