bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcome of multidisciplinary management of adrenocortical carcinoma: a retrospective study
Authors: Zhu Jiawei , Zheng Ziye , Shen Jing , Lian Xin , Miao Zheng , Shen Jie , Zhang Fuquan 
Published: February 2020

Abstract
Background: Adrenocortical Carcinoma (ACC) is a rare and highly malignant tumor with a high recurrence rate. Reoperation to attain negative resection margin is recommended for selected patients. However, there is limited data on the outcome of unresectable recurrent tumor treated with palliative radiotherapy (RT) and transhepatic arterial embolization (TAE).Method: A retrospective analysis of the Peking Union Medical College Hospital was conducted. Patients with pathologically confirmed ACC were included.Result: Ninety-two patients fulfilled the criteria. The 5-year, 3-year, 1-year overall survival (OS) rates were 27.2%, 44.6% and 71.7% respectively, and median OS was 32.1 months. Age < 50 years, ENSAT stage I/II, surgery and negative surgical margin were associated with improved OS (all P <0.05). A Ki67 index < 20% was a predictor of better disease-free interval (P =0.011). Of 51 patients with recurrent ACC, local treatment (reoperation, palliative RT and TAE) improved OS after recurrence from 14.5 months to 41.6 months. Local therapy and mitotane were both prognostic factors for improved OS after recurrence.Conclusion: multidisciplinary treatment is the major therapeutic approach for ACC. Resectable cases should receive complete resection to attain negative margin. Local treatment such as palliative RT and TAE may extend OS for unresectable recurrence.
Postsurgical recurrence and metastasis of rectal cancer with T1N0M0 stage
Authors: Wang Min , Cao Lanqing , He Hongyu , Wang Shuang , Li Jiannan 
Published: February 2020

Abstract
Background: Post-surgical recurrence and metastasis of colorectal cancer (CRC) with T1N0M0 pathological stage hardly occur. Case presentation: We reported a rare case of post-surgical recurrence and distant metastasis of CRC with T1N0M0 pathological stage. Before the first surgery, electric coloscopy and following pathology examination suspected the rectal mass as adenocarcinoma. The patient underwent radical surgery of the rectal tumor. Postsurgical pathology confirmed the diagnosis of rectal adenocarcinoma with the stage of T1N0M0. At about 3.5 years post the first surgery, the chest and abdominal computed tomography (CT) indicated multiple metastasis in both lungs, a small hepatic cyst, gallstones, flaky shadows in the left 10th rib, the thickening of the intestinal wall of sigmoid colon, pelvic fluid, and bone destruction in the sacral area. The pathology confirmed the recurrence of the rectal adenocarcinoma. As a result, sigmoidectomy, recrectomy, and sigmoid colostomy (Hartmman) were performed. Tumor recurrence and metastasis occurred in this case and Hartmann surgery was performed to improve the life quality of the patient. Conclusion: Although, most of the CRC patients with T1N0M0 pathological stage can recover well after radical tumor resection, timely reexamination and specific diagnosis and treatment plan should still be noted.
Prevalence of Female Genital Mutilation among reproductive age women in Ethiopia: Systematic Review and Meta-analysis
Authors: Atlaw Daniel , Seyoum Kenbon , Gezahegn Habtamu 
Published: February 2020

Abstract
Background: - Female genital mutilation (FGM) is the most common harmful traditional practice. Which is characterized by partial or total removal of the female external genitalia for non-therapeutic reasons. Globally, FGM affects about 130 million women and girls. Female Genital cutting (FGC) is a harmful traditional practice which affects the physical and mental health of girls and women.
Methods: - Review and meta-analysis was conducted using the guideline of Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA). Both published and unpublished articles were searched. Articles were searched from different databases like PubMed, Popline, AJOL, EMBASE and gray literature like Google scholar and Google. Articles were searched using terms like “ prevalence ”, “ magnitude”, “female genital cutting” “female genital mutilation”, and “female circumcision” . Joanna Briggs Institute (JBI) Critical Appraisal-Checklist for Analytical Cross Sectional Studies were used to assess the quality of the included paper. Egger’s test and I 2 statistics were used to assess Publication bias and heterogeneity respectively.
Result and discussion: - About thirteen studies with total participants of 7850 were included for systematic review and meta-analysis. The pooled prevalence of female genital mutilation among reproductive age women in Ethiopia was 87.5%: 95% CI (84.25, 90.78). ). I square test statistics showed high heterogeneity (I 2 =94.4, p=0.000) and Egger’s test was done to check for publication bias, but the test has revealed that there is no statistical significant publication bias (p-value=0.374).
Conclusion: - The pooled prevalence of female genital mutilation is high in Ethiopia. Subgroup analysis does not revealed significant difference among different region found in the country.
Identification of a metabolic signature genes predicting overall survival for Head and neck squamous cell carcinoma
Authors: Wu Zenghong , Zhou Wen 
Published: February 2020

Abstract
Background: Head and Neck Squamous Cell Carcinoma (HNSCC) is a common head and neck malignancy that originates from lips, mouth, paranasal sinuses, oropharynx, larynx, nasopharynx, and other pharyngeal cancers. Since high-throughput expression data is now available, it is feasible to use global gene expression data to analyze the relationship between metabolic-related gene expression and clinical outcomes in HNSCC patients. Method: In this study, we used RNA sequencing (RNA-seq) data from cancer genomic maps (TCGA) and validated in the GEO dataset to shine the dysfunctional metabolic microenvironment and given potential biomarkers for metabolic therapy.Results: TCGA database contained 529 patients and 327 metabolic genes (198 upregulated and the largest logFC is CA9; 129 downregulated and the largest logFC is CA6. Cox regression model found 51 prognosis‐related genes and we found the most high-risk gene was LCLAT1 (HR=1.144, 95% CI=1.044~1.251); the most low-risk gene was CHDH (HR=0.580, 95% CI=0.400~0.839). We next study whether metabolic-related genes patterns could serve as an early predictor of incidence of HNSCC by ROC curve and the model demonstrated an AUC of 0.745 in TCGA and 0.618 in GEO. Meanwhile, the predictor of clinicopathological in HNSCC was also analyzed and we found the AUC for age, gender, grade, stage, T, M and N were 0.520, 0.495, 0.568, 0.606, 0.577, 0.476 and 0.673, respectively in TCGA and the AUC for age, gender, stage, T, M, N, smoking and HPV16-pos were 0.599, 0.531, 0.622, 0.606, 0.616, 0.550, 0.614, 0.519 and 0.397 respectively in GEO.Conclusion: Our research found a novel metabolic signature gene for HNSCC prognosis prediction based on the TCGA dataset. Our signatures may reflect metabolic microenvironment disorders and provide useful biomarkers for metabolic therapy and response treatment prediction.
IgG4-related autoimmune hepatitis with pathological characteristics of giant-cell hepatitis: A case report
Authors: Wen Tan You , Sheng Jianhui , Zhou Xingbei 
Published: February 2020

Abstract
Background ：Immunoglobulin (Ig)G4-positive plasma cells are thought to be present in some cases of autoimmune hepatitis (AIH), and giant-cell hepatitis (GCH) or synchronous giant hepatocytes are rare in some patients with AIH. Case presentation : A 68-year-old woman was hospitalized with asthenia, poor appetite, and yellow urine for more than 20 days. accompanied by obvious abnormal liver function, elevated IgG and IgG4 levels. Liver biopsy was performed, and the pathology showed extensive proliferation of fibrous tissue, formation of pseudolobules, moderate and severe interfacial inflammation, bridging necrosis and fibrosis, edema of hepatocytes as rosette-forming cells, multinucleated hepatocytes in each portal area, extensive monocyte lymph infiltration, dense plasma cells in the portal area, and positive plasma cells stained by IgG4 (more than 10 per portal area). The pathological diagnosis was IgG4-related AIH and GCH. Methylprednisolone was administered (20 mg/day). Two weeks later, her liver function gradually improved, and the immune indexes began to decline. Conclusion: we report a case of cirrhosis caused by AIH, which has the characteristics of IgG4 correlation and GCH in the hepatic pathology. Whether there is an inevitable relationship between them remains unclear.
MicroRNA expression profile in the N2 phenotype neutrophils of colorectal cancer and screen of putative key mircRNAs
Authors: Wang Liang , Yang Jun , Huang Jian , Wen Zheng-Qi , Xu Ning , Liu Xuan , Zhang Jian-Hua , Li Wen-Liang 
Published: February 2020

Abstract
Objective: Colorectal cancer (CRC) is one of the most common malignant tumors in the digestive tract, which accounts for 10% of all the malignant tumors in the world. The aim of this study was to identify key genes and miRNAs in CRC diagnosis, prognosis, and therapy and to further explore the potential molecular mechanisms of CRC.Methods: The infiltration and metastasis of neutrophils in Primary colorectal cancer tissue and Paracancerous tissue were observed by immunohistochemical staining. After inducing N2 neutrophils with TGF-β1 in vitro, exosomes were extracted and sequenced, and then the expression differences of microRNAs were screened by using Agilent microRNA microarrays. The data were imported to the Web CARMA for differential expression analysis. The GO and KEGG enrichment analysis were performed using DIANA-MirPath v3.0 using Targetscan database. And the corresponding targets were imported into Gephi for network analysis. The expression level of differentially expressed microRNA using quantitative Realtime polymerase chain reaction (RT-PCR) was validated.Results: A total of 2 miRNAs were found to be associated with N2 neutrophils, in which the expression of hsa-miR-4780 was upregulated and the expression of hsa-miR-3938 was downregulated in N2 neutrophils, compared with the neutrophils. In addition, the results of miRNA-targets networks showed that the hsa-mir-3938 and hsa-mir-4780 could regulate TUSC1 and ZNF197. The expression level of hsa-miR-4780 and hsa-miR-3938 were validated in accordance with the results of RT-PCR.Conclusion: The hsa-mir-3938 and hsa-mir-4780 were differentially expressed between N2 neutrophils and neutrophils. Moreover, the regulation of TUSC1 and ZNF197 by these DEmiRNA established the theoretical basis for the mechanism of N2 type neutrophils regulating the invasion and metastasis of CRC cells, and provided the potential biomarker for prognosis for clinical treatment of CRC
The morphological characteristics and phylogenetic analysis of Pratylenchus vulnus Taiwan strawberry isolate
Authors: Lin Yu-po , Lee Wan-chun , Chung Pei-che , Yang Jiue-in 
Published: February 2020

Abstract

Pratylenchus vulnus was discovered in nematode-distributing fields from symptomatic seedling roots and corresponding rhizosphere soil on strawberry farms in Taiwan. Microscopic measurements and scanning electron microscope observations of both sexes of the nematode coincided with the general morphological descriptions of the species. Four different types of female tail termini were observed, including pointed, digitate, smooth and tapering. Molecular analysis of the ribosomal RNA sequence (SSU, ITS and LSU regions) and the mitochondria COI gene sequences confirmed the species identification. Phylogenetic analysis suggested no specific geographic linkage of the Taiwan population to other previously reported populations.
Cryphodera gayae n. sp. (Nematoda: Heteroderidae), from root of Lagerstroemia indica in Korea
Authors: Kang Heonil , Seo Jongmin , Han Garam , Kim Donggeun , Choi Insoo 
Published: February 2020

Abstract
A new non-cyst forming nematode, Cryphodera gayae n. sp., was found from the root of crapemyrtle (Lagerstroemia indica) in Korea. Female C. gayae n. sp. appears pearly white when young, has protruding vulval lips, flat to concave anus-vulva profile and has three lateral lines in J2s. Morphologically, Cryphodera gayae n. sp. is most similar to C. brinkmani but differ by the tail shape of J2 (sharply pointing with constriction at the middle of hyaline without mucro tip vs. sharply pointing with mucro-like tip), shorter stylet length of J2 (26.8–31.3 µm vs 31.6–35.4 µm), stylet knob shape of J2 (flat posteriorly vs concave), number of eggs in female (64–69 vs 15–45). C. gayae n. sp. differs from C. kalesari by the longer vulva-anus distance (51.9–54.1 µm vs 35 µm), the bigger body size of female (363.1–544.6 µm vs 272–353 µm in length), in J2, the longer tail length (59.6–76.7 µm vs 27–54 µm), longer hyaline length (31.7–47.8 µm vs 18–26 µm) and the shape of tail (sharply pointing with constriction at the middle of hyaline vs narrow rounded without constriction). From C. sinensis, the new species differs by the longer J2 tail length (59.6–76.7 µm vs 52.0–65.0 µm) and longer hyaline portion (31.7–47.8 µm vs 24.5–35.0 µm) of J2. C. gayae n. sp. and can be distinguished from the remaining four species, C. eucalypti, C. podocarpi, C. nothophagi and C. coxi, by the shape of the J2 tail terminus (sharply pointing tip with constriction at the middle of hyaline vs narrow rounded) and the shape of the vulval lips (pronounced protruding vs protruding slightly out of body contour). Molecular analysis shows that C. gayae n. sp. is the most similar to C. sinensis with the identity of sequence 95% on the rRNA LSU D2–D3 segments and the highest match with C. brinkmaini with 88% identity on the ITS region.
Description of Hirschmanniella dicksoni n. sp. (Nematoda: Pratylenchidae) from rhizosphere soil of limpograss from Florida, USA
Authors: Habteweld Alemayehu W. , Akyazi Faruk , Joseph Soumi , Crow William T. , Abebe Eyualem , Mekete Tesfamariam 
Published: February 2020

Abstract

Hirschmanniella dicksoni n. sp. isolated from the rhizosphere soil of limpograss is described and illustrated based on morphology and molecular characters. The new species is characterized by its body length, 1,582 to 1,929 µm, hemispherical lip region with 6 to 7 annules, stylet length 19 to 21 µm, irregularly areolated lateral fields, oval spermatheca filled with rounded sperm, intestine not overlapping the rectum, and tail length 103 to 149 µm, usually with ventral projection with subterminal notch. Males are similar to the females except for their shorter body length and presence of secondary sexual characteristics, with spicules length 19 to 30 µm. The new species is closely related with H. mucronata, H. belli, and H. oryzae. Compared with these three species, the new species has a relatively longer tail and a terminus shape tail with a ventral projection with a subterminal notch. Other morphological and morphometric characteristics that separate these species are as follows: lip region, number of lip annuli, and spicules length. Molecular sequence analysis using the D2–D3 expansion segments of 28S and the ITS rRNA sequences showed that the new species is genetically distinct. D2–D3 sequence of H. dicksoni n. sp. showed 99 and 95% sequence homology with an undescribed species of Hirschmanniella isolated from the Colorado River in Yuma, Arizona, and H. oryzae, respectively. ITS sequence of the new species also showed 88% sequence homology with H. oryzae. The phylogenetic analysis of D2–D3 and ITS region grouped H. dicksoni n. sp., as sister species, with H. oryzae.
Investigation of polar mobile organic compounds (PMOC) removal by reverse osmosis and nanofiltration: rejection mechanism modelling using decision tree
Authors: Teychene B. , Chi F. , Chokki J. , Darracq G. , Baron J. , Joyeux M. , Gallard H. 
Published: February 2020

Abstract
Polar mobile organic compounds (PMOC) are highly polar chemicals and tend to accumulate in short water cycles. Due to their properties, PMOC might be partially eliminated by advanced water treatment technologies. The goal of this study is to investigate the rejection of 22 PMOC (highly mobile and persistent) by reverse osmosis (RO) and nanofiltration (NF) membranes. The impact of transmembrane pressure was evaluated through laboratory-scale cross-flow constant pressure filtration tests. Among the investigated experimental conditions, PMOC rejection with NF at eight bars is comparable to values obtained on RO at 15 bars. Negatively charged PMOC are highly rejected by both RO and NF membranes while guanidine-like compounds exhibit higher passage values and are strongly impacted by transmembrane pressure. In order to model the rejection mechanism, decision tree methodology was employed to link PMOC physicochemical properties to rejection values. Based on laboratory-scale results, decision trees were computed and emphasized that the NF rejection mechanism is governed by electrostatic interaction and sieving effects. In contrast, PMOC rejection on the RO membrane strongly depends on the topological polar surface area (TPSA) of the PMOC. This study suggests that micropollutant TPSA should be more investigated in order to describe RO removal efficiency. Moreover, it is shown that the decision tree is a powerful numerical tool in order to reveal the specific sequence leading to micropollutant removal by RO and NF membranes.
Membrane filtration coupled with wet air oxidation for intensified treatment of biorefractory effluents
Authors: Pinchai C. , Monnot M. , Lefèvre S. , Boutin O. , Moulin P. 
Published: February 2020

Abstract
This work aims to analyse the performances of a new hybrid process: membrane filtration to concentrate biorefractory wastewater before treatment by a hydrothermal process such as wet air oxidation. The aim is to obtain a complete discharge of the effluent in the environment. The three different synthetic wastewaters under study were pharmaceutical wastewater, grey wastewater and bilge wastewater. The results of the membrane filtration showed high retention rates as it could reach between 75% and 100% of total organic carbon retention, more than 99% of turbidity removal and more than 70% of hydrocarbon retention. Moreover, it was possible to achieve high concentration factors comprised between 17 and 40 times. Membrane fouling was chemically reversible regardless of the type of pollution. Then, the treatment of the membrane retentates by wet air oxidation process (300 °C, 15 MPa) could eliminate more than 83% of organic pollution for all the tested effluents. In summary, the hybrid intensified process could finally decrease the volume and the waste load of wastewater before possibly discharging it into the environment.
Analysis of the Clinical Diagnostic Value of GMFB in Cerebral Infarction
Authors: Yuan Zhaohu , Yu Zhiwu , Zhang Yiyu , Yang Huikuan 
Published: February 2020

Background:
Glial Maturation Factor Beta (GMFB) is a highly conserved brain-enriched
protein implicated in immunoregulation, neuroplasticity and apoptosis, processes central to neural injury
and repair following cerebral ischaemia. Therefore, we examined if changes in neurocellular GMFB
expression and release can be used to assess brain injury following ischaemia.

Methods and Results:
Immunofluorescence staining, Western blotting, immunohistochemistry and
ELISA were used to measure GMFB in cultured neurons and astrocytes, rat brain tissues and plasma
samples from stroke model rats and stroke patients, while cell viability assays, TTC staining and micro-
PET were used to assess neural cell death and infarct severity. Immunofluorescence and immunohistochemistry
revealed GMFB expression mainly in astrocyte and neuronal nuclei but also in neuronal
axons and dendrites. Free GMFB concentration increased progressively in the culture medium during
hypoxia-hypoglycaemia treatment. Plasma GMFB concentration increased in rats subjected to middle
cerebral artery occlusion (MCAO, a model of stroke-reperfusion) and in stroke patients. Plasma
GMFB in MCAO model rats was strongly correlated with infarct size (R2=0.9582). Plasma GMFB
concentration was also markedly elevated in stroke patients within 24 h of onset and remained elevated
for more than one week. Conversely, plasma GMFB elevations were not significant in myocardial infarct
patients and stroke patients without infarction.

Conclusion:
GMFB has the prerequisite stability, expression specificity and response dynamics to
serve as a reliable indicator of ischaemic injury in animal models and stroke patients. Plasma GMFB
may be a convenient non-invasive adjunct to neuroimaging for stroke diagnosis and prognosis.
Novel Phospholipid-Based Labrasol Nanomicelles Loaded Flavonoids for Oral Delivery with Enhanced Penetration and Anti-Brain Tumor Efficiency
Authors: Wang Gang , Wang Junjie , Guan Rui 
Published: February 2020

Background:
Owing to the rich anticancer properties of flavonoids, there is a need for their
incorporation into drug delivery vehicles like nanomicelles for safe delivery of the drug into the brain
tumor microenvironment.

Objective:
This study, therefore, aimed to prepare the phospholipid-based Labrasol/Pluronic F68 modified
nano micelles loaded with flavonoids (Nano-flavonoids) for the delivery of the drug to the target
brain tumor.

Methods:
Myricetin, quercetin and fisetin were selected as the initial drugs to evaluate the biodistribution
and acute toxicity of the drug delivery vehicles in rats with implanted C6 glioma tumors after oral administration,
while the uptake, retention, release in human intestinal Caco-2 cells and the effect on the brain
endothelial barrier were investigated in Human Brain Microvascular Endothelial Cells (HBMECs).

Results:
The results demonstrated that nano-flavonoids loaded with myricetin showed more evenly
distributed targeting tissues and enhanced anti-tumor efficiency in vivo without significant cytotoxicity
to Caco-2 cells and alteration in the Trans Epithelial Electric Resistance (TEER). There was no pathological
evidence of renal, hepatic or other organs dysfunction after the administration of nanoflavonoids,
which showed no significant influence on cytotoxicity to Caco-2 cells.

Conclusion:
In conclusion, Labrasol/F68-NMs loaded with MYR and quercetin could enhance antiglioma
effect in vitro and in vivo, which may be better tools for medical therapy, while the pharmacokinetics
and pharmacodynamics of nano-flavonoids may ensure optimal therapeutic benefits.
Calcium/Copper Alginate Framework Doped with CuO Nanoparticles as a Novel Adsorbent for Micro-extraction of Benzodiazepines from Human Serum
Authors: Rahbar Nadereh , Ahmadi Fatemeh , Ramezani Zahra , Nourani Masoumeh 
Published: February 2020

Background:Sample preparation is one of the most challenging phases in pharmaceutical analysis, especially in biological matrices, affecting the whole analytical methodology.Objective:In this study, a new Ca(II)/Cu(II)/alginate/CuO Nanoparticles Hydrogel Fiber (CCACHF) was synthesized through a simple, green procedure and applied for fiber micro solid-phase extraction (FMSPE) of diazepam (DIZ) and oxazepam (OXZ) as model drugs prior to high-performance liquid chromatography-UV detection (HPLC-UV).Methods:Composition and morphology of the prepared fiber were characterized and the effect of main parameters on the fiber fabrication and extraction efficiency have been studied and optimized.Results:In optimal conditions, calibration curves were linear, ranging between 0.1–500 μg L−1with regression coefficients of 0.9938 and 0.9968. Limit of Detection (LOD) (S/N=3) and Limit of Quantification (LOQ) (S/N=10) of the technique for DIZ and OXZ were 0.03 to 0.1 μg L−1. Within-day and between-day Relative Standard Deviations (RSDs) for DIZ and OXZ were 6.0–12.5% and 3.3–9.4%, respectively.Conclusion:The fabricated adsorbent has been substantially employed to the extraction of selected benzo-diazepines (BZDs) from human serum real specimens and the obtained recoveries were also satisfactory (82.1-109.7%).