bluuebunny/crossref_metadata_2025_split huggingface dataset:
LCI1, a Chlamydomonas reinhardtii plasma membrane protein, functions in active CO2 uptake under low CO2
Authors: Kono Alfredo , Spalding Martin H. 
Published: April 2020

SummaryIn response to high CO2 environmental variability, green algae, such as Chlamydomonas reinhardtii, have evolved multiple physiological states dictated by external CO2 concentration. Genetic and physiological studies demonstrated that at least three CO2 physiological states, a high CO2 (0.5–5% CO2), a low CO2 (0.03–0.4% CO2) and a very low CO2 (< 0.02% CO2) state, exist in Chlamydomonas. To acclimate in the low and very low CO2 states, Chlamydomonas induces a sophisticated strategy known as a CO2‐concentrating mechanism (CCM) that enables proliferation and survival in these unfavorable CO2 environments. Active uptake of Ci from the environment is a fundamental aspect in the Chlamydomonas CCM, and consists of CO2 and HCO3– uptake systems that play distinct roles in low and very low CO2 acclimation states. LCI1, a putative plasma membrane Ci transporter, has been linked through conditional overexpression to active Ci uptake. However, both the role of LCI1 in various CO2 acclimation states and the species of Ci, HCO3– or CO2, that LCI1 transports remain obscure. Here we report the impact of an LCI1 loss‐of‐function mutant on growth and photosynthesis in different genetic backgrounds at multiple pH values. These studies show that LCI1 appears to be associated with active CO2 uptake in low CO2, especially above air‐level CO2, and that any LCI1 role in very low CO2 is minimal.
Arabidopsis thaliana SURFEIT1‐like genes link mitochondrial function to early plant development and hormonal growth responses
Authors: Gras Diana E. , Mansilla Natanael , Rodríguez Carina , Welchen Elina , Gonzalez Daniel H. 
Published: April 2020

SUMMARYMutations in SURFEIT1 (SURF1) genes affect cytochrome c oxidase (COX) levels in different prokaryotic and eukaryotic organisms. In this work, we report that Arabidopsis thaliana has two genes that potentially encode SURF1 proteins, as a result of a duplication that took place in Brassicaceae. Both genes encode mitochondrial proteins and mutation in AtSURF1a causes embryonic lethality. Mutation in AtSURF1b, instead, causes defects in hypocotyl elongation under growth‐stimulating conditions, such as low light intensity, increased ambient temperature and incubation with glucose. Mutants in AtSURF1b show reduced expression of the auxin reporter DR5:GUS and increased levels of the gibberellin reporter GFP‐RGA, suggesting that auxin and gibberellin homeostasis are affected. In agreement, growth defects caused by AtSURF1b mutation can be overcome by treatment with indole‐3‐acetic acid and gibberellin A3, and also by increasing expression of the auxin biosynthesis gene YUC8 or the transcription factor PIF4, which shows lower abundance in AtSURF1b‐deficient plants. Mutants in AtSURF1b display lower COX levels, higher alternative oxidase and superoxide levels, and increased expression of genes that respond to mitochondrial dysfunction. Decreased hypocotyl growth and DR5:GUS expression can be reversed by treatment with reduced glutathione, suggesting that redox changes, probably related to mitochondrial dysfunction, are responsible for the effect of AtSURF1b deficiency on hormone responses. The results indicate that changes in AtSURF1b affect mitochondrial function and the production of reactive oxygen species, which, in turn, impinges on a growth regulatory circuit that involves auxin, gibberellins and the transcription factor PIF4.
What attracts foreign direct investment into autocratic states? Regime time horizon and institutional design
Authors: Cui Lin , Moon Chungshik 
Published: April 2020

AbstractStates play a critical role in designing institutions to facilitate international business. We study the effect of autocratic states' time horizons on their attraction of foreign direct investment (FDI) through designing domestic and international institutions. We argue that autocrats with a long time horizon tend to build credibledomesticcommitment‐institutions that attract foreign investors; however, they are also likely to affect the design of commitment carve‐outs ininternationalinstitutions, in particular bilateral investment treaties, thus weakening their institutional effect on foreign investment. We test these dual effects of regime time horizon on FDI inflow using data from 80 autocratic states over a 33‐year period and find substantial support for our arguments.
Comparative Analysis of Glycosylated and Nonglycosylated Filarial Homologues of the 20-Kilodalton Retinol Binding Protein fromOnchocerca volvulus(Ov20)
Authors: Nirmalan Niroshini , Cordeiro N. J. V. , Kläger Sabine L. , Bradley Janette E. , Allen Judith E. 
Published: April 2020

ABSTRACTOv20 is a structurally novel 20-kDa retinol binding protein secreted byOnchocerca volvulus. Immunological and biological investigation of this protein has been hampered by the inability to maintainO. volvulusin a laboratory setting. In an effort to find a system more amenable to laboratory investigation, we have cloned, sequenced, and expressed cDNA encoding homologues of Ov20 from two closely related filarial species,Brugia malayi(Bm20) andAcanthocheilonema viteae(Av20). Sequence comparisons have highlighted differences in glycosylation of the homologues. We present here an analysis of mouse immune responses to Ov20, Bm20, and Av20. The results suggest a strong genetic restriction in response to native Bm20 that is overcome when recombinant, nonnative material is used. Reactivity of human filarial sera to the three recombinant proteins confirmed previous specificity studies with Ov20 but highlighted important differences in the reactivity patterns of theO. volvulusandB. malayihomologues that may be due to differences in glycosylation patterns. Ov20 is a dominant antigen in infected individuals, while Bm20 is not. The availability of theB. malayihomologue enabled us to use defined murine reagents and inbred strains for genetic analysis of responsiveness in a way that is not possible for Ov20. However, the close sequence similarity between Ov20 and Av20 suggests that theA. viteaemodel may be more suited to the investigation of the biological functions of Ov20.
Production of Tumor Necrosis Factor Alpha in Human T Lymphocytes by Staphylococcal Enterotoxin B Correlates with Toxin-Induced Proliferation and Is Regulated through Protein Kinase C
Authors: Yan Zhengyin , Yang David C. H. , Neill Roger , Jett Marti 
Published: April 2020

ABSTRACTThe superantigen staphylococcal enterotoxin B (SEB) simultaneously binds both the major histocompatibility complex (MHC) class II receptor on monocytes and the T-cell receptor (TCR) on T lymphocytes, resulting in a range of cell responses including induction of tumor necrosis factor alpha (TNF-α). In this study, we have used mixed cultures of human peripheral blood monocytes and lymphocytes to investigate biochemical events controlling SEB induction of TNF-α. TNF-α production induced by SEB in mixed cultures is more closely associated with T cells than with monocytes: (i) a TCR-binding-site mutant of SEB (N23F) is less active in TNF-α induction than an MHC class II receptor-binding-site mutant (F44R), and (ii) flow cytometric analysis indicated that SEB induced TNF-α production in T cells but not in monocytes. Pretreatment of cells with inhibitors of signal transduction pathways was employed to further define events in SEB-induced TNF-α production. Neither protein kinase A inhibitors nor two protein tyrosine kinase inhibitors altered SEB-induced TNF-α production. In contrast, SEB induced protein kinase C (PKC) translocation, and pretreatment of cultures with inhibitors of PKC blocked TNF-α induction. Alteration of levels of diacylglycerol (DAG), an activator of PKC, by treatment with inhibitors of phospholipase C or DAG kinase also altered SEB-induced TNF-α production. These data suggest that PKC activation plays a critical role in SEB-induced TNF-α production in human T cells.
Attenuated Expression of the
mga
Virulence Regulon in an M Serotype 50 Mouse-Virulent Group A Streptococcal Strain
Authors: Yung Der-Li , McIver Kevin S. , Scott June R. , Hollingshead Susan K. 
Published: April 2020

ABSTRACT

The attenuated expression of virulence genes found in a group A streptococcal strain that is naturally pathogenic for mice was postulated to result from a defect in the strain's multigene regulator, Mga. The sequence of the
mga
gene reveals three amino acid changes in the gene product that might affect protein function. The defect in the
mga
gene was complemented by providing either the closely similar
mga4
allele or a more divergent
mga1
allele in
trans
. Complementation increased the amount of
emm50
transcript and the quantity of surface-extractable M protein, restoring virulence function.
Real-time cosmology with SKA
Authors: Liu Yan , Zhang Jing-Fei , Zhang Xin 
Published: April 2020

AbstractIn this work, we investigate what role the redshift-drift data of the Square Kilometre Array (SKA) will play in the cosmological parameter estimation in the future. To test the constraint capability of the redshift-drift data of SKA-only, the $$\Lambda $$ΛCDM model is chosen as a reference model. We find that using the SKA1 mock data, the $$\Lambda $$ΛCDM model can be loosely constrained, while the model can be well constrained when the SKA2 mock data are used. When the mock data of SKA are combined with the data of the European Extremely Large Telescope (E-ELT), the constraints can be significantly improved, becoming almost as good as the data combination of the type Ia supernovae observation (SN), the cosmic microwave background observation (CMB), and the baryon acoustic oscillations observation (BAO). Furthermore, we explore the impact of the redshift-drift data of SKA on the basis of SN+CMB+BAO+E-ELT in the $$\Lambda $$ΛCDM model, the wCDM model, the CPL model, and the HDE model. We find that the redshift-drift measurement of SKA could help to significantly improve the constraints on dark energy and could break the degeneracy between the cosmological parameters. Therefore, we conclude that redshift-drift observation of SKA would provide a good improvement in the cosmological parameter estimation in the future and has the enormous potential to be one of the most competitive cosmological probes in constraining dark energy.
Twenty Years of the Protection of Civilians in UN Peace Operations
Authors: Hunt Charles T. , Zimmerman Shannon 
Published: April 2020

The Protection of Civilians (PoC) has been part of United Nations (UN) peace operations for twenty years. Today, PoC is irrefutably a ‘centre of gravity’ for how UN peace operations see and portray themselves. Despite negative perceptions, a great deal of progress has been made in how missions prepare for and respond to the demands of protection mandates. For the vulnerable populations they serve, mandates to protect raise expectations and provide hope that peacekeepers will safeguard them. Yet efforts to implement PoC mandates have encountered a range of problems, which peace operations have struggled to address. This article critically reflects on the past two decades of promoting, planning for and practicing protection in UN peace operations. It argues that while the achievements are many, significant challenges remain and much more must be done to deliver on this cardinal obligation.
Experimental Contrastive Pragmatics Using Robots
Authors: Fischer Kerstin , Prondzinska Alicja Depka 
Published: April 2020

Abstract
In this paper, we explore how robots can be used to study pragmatic strategies across a number of languages. Robots can assume many of the roles played by human interaction partners in a range of situations. They can be programmed to produce specific behaviours, each time repeating a behaviour in an identical way for as often as necessary. Thus, robots can be useful tools for investigating human behaviour in certain situations and even in cross-cultural contexts. We explore this use of robots in two case studies – one which investigates the delivery of bad news in Danish, German and English, and one which examines the giving of feedback in Danish, German and Polish. In both studies, systematic intercultural differences become apparent in the pragmatic strategies that are adopted. On the basis of the results, we discuss the advantages, potential pitfalls and possible solutions of using robots in the study of contrastive pragmatics.
TFPI2 suppresses breast cancer progression through inhibiting TWIST-integrin α5 pathway
Authors: Zhao Danyi , Qiao Jingjing , He Hongmei , Song Jincheng , Zhao Shanshan , Yu Jing 
Published: April 2020

Abstract
Background
Tissue factor pathway inhibitor 2 (TFPI2) participates in carcinogenesis of various tumors, and is associated with poor survival of breast cancer patients. However, the effect and underlying mechanism of TFPI2 on breast cancer progression remains to be investigated.

Methods
The expression level of TFPI2 in breast cancer tissues and cell lines was examined via qRT-PCR (quantitative real-time polymerase chain reaction) and immunohistochemistry. CCK8 (Cell Counting Kit-8), colony formation, wound healing or transwell assays were used to detect cell viability, proliferation, migration or invasion, respectively. In vivo subcutaneous xenotransplanted tumor model was established to detect tumorigenic function of TFPI2, and the underlying mechanism was evaluated by immunohistochemistry and western blot.

Results
TFPI2 was down-regulated in breast cancer tissues and cell lines, and was associated with poor prognosis of patients diagnosed with breast cancer. Over-expression of TFPI2 inhibited cell viability, proliferation, migration and invasion of breast cancer cells. Mechanistically, Twist-related protein 1 (TWIST1) was negatively associated with TFPI2 in breast cancer patients, whose expression was decreased by TFPI2 over-expression or increased by TFPI2 knockdown. Moreover, TWIST1 could up-regulate integrin α5 expression. Functional assays indicated that the inhibition abilities of TFPI2 over-expression on breast cancer progression were reversed by TWIST1 over-expression. In vivo subcutaneous xenotransplanted tumor model also revealed that over-expression of TFPI2 could suppress breast tumor growth via down-regulation of TWIST1-mediated integrin α5 expression.

Conclusions
TFPI2 suppressed breast cancer progression through inhibiting TWIST-integrin α5 pathway, providing a new potential therapeutic target for breast cancer treatment.
Evaluation of double-folded meatal-based flap (modified Mathieu) in redo circumcised distal penile hypospadias: a prospective multicenter study
Authors: Nagla Salah , Radwan Mohammed , Alhefnawy Mohamed , Ghaith Ahmed , Hagrass Ayman M. , Shalaby Mohammed , Awad Mohammed , Negm Mohamed Ahmed , Elgamal Samir 
Published: April 2020

Abstract
Background
Sometimes it becomes a dilemma to repair a failed circumcised distal penile hypospadias with partial or complete wound dehiscence. In many of these cases, redo operations need a graft or a flap. The aim of this study is to evaluate the double-folded meatal-based flap in redo circumcised hypospadias with shallow urethral plate and a small glans penis.

Results
From September 2015 to August 2018, we prospectively studied the double-folded meatal-based flap in 56 failed circumcised distal hypospadias without penile curvature with shallow urethral plate and a small glans penis. Distally folded Mathieu flap was done. Forty-seven cases only had completed the study. We had 19 patients with distal penile hypospadias, 19 with sub-coronal hypospadias, and 9 with coronal hypospadias. Median operative time was 50 (range 40–80 min). The median length of the double-folded flap was 14 (range 10–18 mm). The median follow-up was 12 (range 1–33 months). The success of the operation means that there is an absence of any complications. Our success rate was 73%. We had 8 patients with meatal retraction, one fistula, and one patient with wound disruption. Only 3 patients (6.4%) needed re-operation: one case with meatal retraction, another with fistula, and a third with complete wound disruption.

Conclusion
Distally folded meatal-based flap is an additional option in redo circumcised hypospadias with shallow urethral plate, small glans penis, and pliable anterior penile skin with a considerable high percent of meatal recession.
A study of optimal concentration range and time window of sevoflurane preconditioning for brain protection in MCAO rats
Authors: Deng Ying , Shi Chengmei , Gu Yi , Yang Ning , Xu Mao , Xu Ting , Guo Xiangyang 
Published: April 2020

Abstract
Background
Sevoflurane preconditioning improves brain function in MCAO rats, and there are several methods for determining appropriate concentration and time windows for preconditioning. This study investigated the brain protective effects with a single sevoflurane preconditioning at different concentrations and different time windows on MCAO rats.

Methods
Adult Sprague-dawley rats were randomly assigned to 14 groups. The rats in the sevoflurane preconditioning group inhaled 0.5 MAC, 1.0 MAC, and 1.3 MAC sevoflurane, respectively for 3 h, and then MCAO models were established at 6 h, 12 h, 24 h, and 48 h. MCAO and sham groups underwent no preconditioning with sevoflurane. The neurological severity score, cerebral infarct volume and brain water content of the rats were measured 24 h after reperfusion.

Results
After inhalation of 1.3 MAC sevoflurane for 3 h of preconditioning, the MCAO model was established after 24 h. This preconditioning improved the neurological severity score, reduce cerebral infarct volume and brain water content in MCAO rats. After inhalation of 1.0 MAC sevoflurane for 3 h of preconditioning, MCAO model established after 24 h reduced the cerebral infarct volume and brain water content of MCAO rats, but the neurological severity score showed no significant improvement, and no significant brain protective effects were observed at other concentrations and time windows.

Conclusions
These results suggested that after inhalation of 1.3 MAC sevoflurane for 3 h of preconditioning, MCAO model established after 24 h demonstrated significant brain protective effects in MCAO rats.
Clinical utility of genetic testing in the early diagnosis of Danon disease mimicking hypertrophic cardiomyopathy: a case report
Authors: Novelli Valeria , Bisignani Antonio , Pelargonio Gemma , Primiano Guido , Narducci Maria Lucia , Palmieri Vincenzo , Tiziano Francesco Danilo , Zeppilli Paolo , Servidei Serenella , Crea Filippo , Genuardi Maurizio 
Published: April 2020

Abstract
Background
Danon disease (OMIM 300257) is an X-linked lysosomal storage disorder, characterized by hypertrophic cardiomyopathy (HCM), skeletal myopathy, variable intellectual disability, and other minor clinical features.
This condition accounts for ~ 4% of HCM patients, with a more severe and early onset phenotype in males, causing sudden cardiac death (SCD) in the first three decades of life.
Genetic alterations in the LAMP2 gene are the main cause of this inherited fatal condition. Up to date, more than 100 different pathogenic variants have been reported in the literature. However, the majority of cases are misdiagnosed as HCM or have a delay in the diagnosis.

Case presentation
Here, we describe a young boy with an early diagnosis of HCM. After 2 episodes of ventricular fibrillation within 2 years, genetic testing identified a novel LAMP2 pathogenic variant. Subsequently, further clinical evaluations showing muscle weakness and mild intellectual disability confirmed the diagnosis of Danon disease.

Conclusions
This report highlights the role of genetic testing in the rapid diagnosis of Danon disease, underscoring the need to routinely consider the inclusion of LAMP2 gene in the genetic screening for HCM, since an early diagnosis of Danon disease in patients with a phenotype mimicking HCM is essential to plan appropriate treatment, ie cardiac transplantation.
Assessment of successful percutaneous mitral commissurotomy by MRproANP and sCD146
Authors: Badoz Marc , Arrigo Mattia , Mogenet Anne-Claire , Sadoune Malha , Meneveau Nicolas , Mebazaa Alexandre , Seronde Marie-France 
Published: April 2020

Abstract
Background
We studied the course of plasma concentrations of 4 cardiovascular biomarkers: natriuretic peptides (BNP, NT-proBNP; mid-regional (MR) pro-atrial NP); and soluble endothelial CD146 (sCD146), in patients with severe mitral valve stenosis undergoing percutaneous mitral commissurotomy (PMC) to identify potential markers of procedural success.

Methods
Biomarkers were tested in 40 patients the day before and the day after PMC. Success was defined as mitral valve area ≥ 1.5 cm2; or an increase of ≥0.5 cm2 in mitral valve area associated with echocardiographic mitral regurgitation <grade 3–4 post-PMC.

Results
Average age was 63.5 ± 12.7 years; 32(80%) were female. Before PMC, mean valve area was 1.1 ± 0.2 cm2, mean gradient 9.1 ± 3.5 mmHg. PMC was successful in 30 (75%) and unsuccessful in 10 (25%). PMC yielded a significant reduction in MR-proANP and sCD146, driven by a significant reduction in these biomarkers in patients with successful procedure, whereas no reduction was observed in patients with unsuccessful procedure. A significant correlation was found between changes in plasma sCD146 and the relative change in mitral valve area. Elevated pre-procedural sPAP correlated with high sCD146, and accordingly, a significant correlation between the decrease in sPAP and sCD146 after PMC was shown.

Conclusions
MR-proANP and plasma sCD146 decreased significantly immediately after successful PMC. They appear to be markers of immediate success of PMC and of the hemodynamic improvement achieved by this procedure in patients with MS.

Trial registration
This study is part of the cohorts registered with ClinicalTrials.gov on June 16, 2011 under the number NCT01374880.
Validation of an algorithm based on administrative data to detect new onset of atrial fibrillation after cardiac surgery
Authors: Bourgon Labelle Jonathan , Farand Paul , Vincelette Christian , Dumont Myriam , Le Blanc Mathilde , Rochefort Christian M. 
Published: April 2020

Abstract
Introduction
Postoperative atrial fibrillation (POAF) is a frequent complication of cardiac surgery associated with important morbidity, mortality, and costs. To assess the effectiveness of preventive interventions, an important prerequisite is to have access to accurate measures of POAF incidence. The aim of this study was to develop and validate such a measure.

Methods
A validation study was conducted at two large Canadian university health centers. First, a random sample of 976 (10.4%) patients who had cardiac surgery at these sites between 2010 and 2016 was generated. Then, a reference standard assessment of their medical records was performed to determine their true POAF status on discharge (positive/negative). The accuracy of various algorithms combining diagnostic and procedure codes from: 1) the current hospitalization, and 2) hospitalizations up to 6 years before the current hospitalization was assessed in comparison with the reference standard. Overall and site-specific estimates of sensitivity, specificity, positive (PPV), and negative (NPV) predictive values were generated, along with their 95%CIs.

Results
Upon manual review, 324 (33.2%) patients were POAF-positive. Our best-performing algorithm combining data from both sites used a look-back window of 6 years to exclude patients previously known for AF. This algorithm achieved 70.4% sensitivity (95%CI: 65.1–75.3), 86.0% specificity (95%CI: 83.1–88.6), 71.5% PPV (95%CI: 66.2–76.4), and 85.4% NPV (95%CI: 82.5–88.0). However, significant site-specific differences in sensitivity and NPV were observed.

Conclusion
An algorithm based on administrative data can identify POAF patients with moderate accuracy. However, site-specific variations in coding practices have significant impact on accuracy.