bluuebunny/crossref_metadata_2025_split huggingface dataset:
The emergence of colistin-resistant Escherichia coli in chicken meats in Nepal
Authors: Joshi Prabhu Raj , Thummeepak Rapee , Leungtongkam Udomluk , Pooarlai Renukar , Paudel Saroj , Acharya Mahesh , Dhital Samita , Sitthisak Sutthirat 
Published: November 2019

ABSTRACT
The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.33%), of which 16 (26.66%) were colistin-resistant and harboured the mcr-1 gene. All isolates were characterised by antibiotic susceptibility testing, the presence of antibiotic resistance genes, phylogenetic analysis and plasmid replicon typing. Most of the colistin-resistant E. coli had the antibiotic resistant pattern CIP/CN/SXT/TE (43.75%). Coexistence of tet, qnr, sul and dfr genes was detected in both colistin-resistant and -susceptible E. coli. Most colistin-resistant E. coli strains belonged to phylogroup C, whereas 10% of isolates belonged to phylogroup D. Inc FIB was the dominant plasmid Inc type in the isolates. Dissemination of antibiotic-resistant E. coli in raw meats is a public health concern in Nepal and requires further investigation to ascertain the sources of contamination.
Genome Assembly of the Dogface Butterfly Zerene cesonia
Authors: Rodriguez-Caro Luis , Fenner Jennifer , Benson Caleb , Van Belleghem Steven M , Counterman Brian A 
Published: November 2019

Abstract
Comparisons of high-quality, reference butterfly, and moth genomes have been instrumental to advancing our understanding of how hybridization, and natural selection drive genomic change during the origin of new species and novel traits. Here, we present a genome assembly of the Southern Dogface butterfly, Zerene cesonia (Pieridae) whose brilliant wing colorations have been implicated in developmental plasticity, hybridization, sexual selection, and speciation. We assembled 266,407,278 bp of the Z. cesonia genome, which accounts for 98.3% of the estimated 271 Mb genome size. Using a hybrid approach involving Chicago libraries with Hi-Rise assembly and a diploid Meraculous assembly, the final haploid genome was assembled. In the final assembly, nearly all autosomes and the Z chromosome were assembled into single scaffolds. The largest 29 scaffolds accounted for 91.4% of the genome assembly, with the remaining ∼8% distributed among another 247 scaffolds and overall N50 of 9.2 Mb. Tissue-specific RNA-seq informed annotations identified 16,442 protein-coding genes, which included 93.2% of the arthropod Benchmarking Universal Single-Copy Orthologs (BUSCO). The Z. cesonia genome assembly had ∼9% identified as repetitive elements, with a transposable element landscape rich in helitrons. Similar to other Lepidoptera genomes, Z. cesonia showed a high conservation of chromosomal synteny. The Z. cesonia assembly provides a high-quality reference for studies of chromosomal arrangements in the Pierid family, as well as for population, phylo, and functional genomic studies of adaptation and speciation.
Does Obesity Increase the Risk and Severity of Falls in People Aged 60 Years and Older? A Systematic Review and Meta-analysis of Observational Studies
Authors: G R Neri Silvia , S Oliveira Juliana , B Dario Amabile , M Lima Ricardo , Tiedemann Anne 
Published: November 2019

AbstractBackgroundRecent investigations suggest that obesity may be associated with an increased risk of falls; however, this theory has yet to be definitively confirmed. This systematic review and meta-analysis examined the strength of the association between obesity and falls, multiple falls, fall-related injuries, and fall-related fractures among older adults.MethodsMEDLINE, Embase, CINAHL, PsycINFO, SPORTDiscus, LILACS, and Web of Science databases were searched to identify observational studies that assessed the association between obesity and fall-related outcomes in participants aged 60 years and older. Two independent reviewers performed data extraction and quality assessment. Relative risks and 95% confidence intervals (CI) were pooled using random effect meta-analyses.ResultsThirty-one studies including a total of 1,758,694 participants were selected from 7,815 references. Pooled estimates showed that obese older adults have an increased risk of falls compared with nonobese counterparts (24 studies; relative risk: 1.16; 95% CI: 1.07–1.26; I2: 90%). Obesity was also associated with an increased risk of multiple falls (four studies; relative risk: 1.18; 95% CI: 1.08–1.29; I2: 0%). There was no evidence, however, of an association between obesity and fall-related injuries (seven studies; relative risk: 1.04; 95% CI: 0.92–1.18; I2: 65%). Fall-related fractures were reported in only one study, which demonstrated a lower risk of hip fracture with obesity (odds ratio: 0.65; 95% CI: 0.63–0.68).ConclusionsObesity increases the risk of falls and multiple falls in people aged 60 years and older; however, there is insufficient evidence of an association with fall-related injuries or fractures. Prevention and treatment of obesity may play a role in preventing falls in older age.
Actor–Partner Effects of Childhood Disadvantage on Later Life Subjective Well-Being Among Individuals in Coresidential Unions
Authors: Lam Jack 
Published: November 2019

Abstract

Objectives
Prior research on cumulative disadvantage has primarily focused on individuals’ own childhood adversity for their later-life outcomes. Nevertheless, partner’s childhood disadvantage may also shape respondent’s later-life well-being.

Methods
Drawing on a household-level dataset, I examine respondent’s own childhood adversity as well as their partner’s childhood adversity (poor childhood health, parental divorce, or father’s long-term unemployment) on respondent’s subjective well-being, at aged 50 and older.

Results
Findings from the actor-partner interdependence model (APIM) show poor childhood health of the male partner as associated with worse mental health and self-rated health of the female partner in later life. For both outcome measures, the partner effects were attenuated after adjusting for the female partner’s report of perceived social support. For self-rated health, adjusting for variation in the presence of a chronic illness and household income also attenuated the association.

Discussion
Partnered individuals are nested within a specific context, whereby stress and implications of early life disadvantage may be conceptualized at the couple-level. Future research that assesses how early life experiences of individuals may have implications for family members’ later-life well-being may be valuable.
Integration of bioenergetics in an individual-based model to hindcast anchovy dynamics in the Bay of Biscay
Authors: Bueno-Pardo Juan , Petitgas Pierre , Kay Susan , Huret Martin 
Published: November 2019

Abstract
The population of European anchovy of the Bay of Biscay collapsed at the beginning of the 21st century, causing the closure of its fishery between 2005 and 2010. In order to study both the human and environmental causes of the anchovy population dynamics, an approach coupling individual bioenergetics to an individual-based model was applied between 2000 and 2015. This modelling framework was forced with outputs from a physical–biogeochemical model. In addition to a base-case scenario with realistic forcing, alternative scenarios were run without inter-annual variability in either fishing mortality or environmental conditions. During the decrease in population biomass, a high fishing pressure coincided with a combination of environmental variables promoting the appearance of large individuals that could not survive severe winters because of their high energetic demands. The recovery of the population was favoured by a period of warm years with abundant food favouring the winter survival of age 1 individuals, in coincidence with the closure of the fishery. Our modelling approach also allows to test the consequences of a retrospective implementation of the current harvest control rule from 2000 which, according to our results, would have prevented the collapse of the population and avoided the fishery closure.
Reduced Consolation Behaviors in Physically Stressed Mandarin Voles: Involvement of Oxytocin, Dopamine D2, and Serotonin 1A Receptors Within the Anterior Cingulate Cortex
Authors: Li Lai-Fu , Yuan Wei , He Zhi-Xiong , Ma Huan , Xun Yu-Feng , Meng Ling-Rong , Zhu Si-Jing , Wang Li-Min , Zhang Jing , Cai Wen-Qi , Zhang Xue-Ni , Guo Qian-Qian , Lian Zhen-Min , Jia Rui , Tai Fa-Dao 
Published: November 2019

AbstractBackgroundConsolation is a type of empathy-like behavior that has recently been observed in some socially living rodents. Despite the growing body of literature suggesting that stress affects empathy, the relationship between stress and consolation remains understudied at the preclinical level. Here, we examined the effects of chronic emotional stress or physical stress exposure on consolation and emotional behaviors by using the socially monogamous mandarin vole (Microtus mandarinus) in both males and females.Method/ResultsPhysical stress voles were exposed to 14-day social defeat stress, whereas emotional stress voles vicariously experienced the defeat of their partners. We found that physical stress, but not emotional stress, voles showed reduced grooming toward their defeated partners and increased anxiety- and despair-like behaviors. Meanwhile, physical stress voles exhibited decreased neural activity in the anterior cingulate cortex, which is centrally involved in empathy. The densities of oxytocin receptors, dopamine D2 receptors, and serotonin 1A-receptors within the anterior cingulate cortex were significantly decreased in the physical stress group compared with controls. All the behavioral and physiological changes were similar between the sexes. Finally, we found that the reduced consolation behavior and some anxiety-like syndromes in physical stress voles could be alleviated by pretreatment with an oxytocin receptor, D2 receptors, or serotonin 1A-receptor agonist within the anterior cingulate cortex, whereas injections of corresponding receptor antagonists to the control voles decreased the consolation behavior and increased some anxiety-like behaviors.ConclusionsOur results indicated that chronic physical stress exposure impaired consolation and induced anxiety-like behaviors in mandarin voles and oxytocin receptors, 5-HT1A receptors, and D2 receptors within the anterior cingulate cortex may play important roles in these processes.
Distance-based nearest neighbour forecasting with application to exchange rate predictability
Authors: Kyriazi Foteini , Thomakos Dimitrios D 
Published: November 2019

AbstractForecasting non-stationary time series, especially when the data generating processes contains a random walk component, is a difficult and sometimes impossible task. In this paper we suggest an intuitive, computationally fast and expedient way of forecasting time series of the above type using distance-based nearest neighbours (NN). We exploit to advantage the path and scale dependence present in a random walk model and so we provide a number of theoretical results (a) on the distances used for selecting the NN, (b) on a number of new forecasting models that use these distances and (c) on the properties of the resulting forecasts. We illustrate the efficacy of our method via a comprehensive empirical application on time series of exchange rates and commodities, where we present the resulting performance enhancements and discuss the importance of such results in a decision-making context, linking our forecasting approach with management mathematics and predictive analytics problems.
On the Modular Completion of Certain Generating Functions
Authors: Bringmann Kathrin , Ehlen Stephan , Schwagenscheidt Markus 
Published: November 2019

Abstract
We complete several generating functions to non-holomorphic modular forms in two variables. For instance, we consider the generating function of a natural family of meromorphic modular forms of weight two. We then show that this generating series can be completed to a smooth, non-holomorphic modular form of weights $\frac 32$ and two. Moreover, it turns out that the same function is also a modular completion of the generating function of weakly holomorphic modular forms of weight $\frac 32$, which prominently appear in work of Zagier [27] on traces of singular moduli.
Champion Coaching: How to Teach Ultrasound-Guided Breast Procedures
Authors: Carpentier Bianca M , Mainiero Martha B 
Published: November 2019

Abstract
Teaching ultrasound-guided percutaneous breast interventions to a trainee may be a challenge, given the often high-anxiety environment in the subspecialty of breast imaging. This article aims to teach an organized approach, including simulation practice with an emphasis on consistent feedback and role modeling, as well as practical and relevant physics, to help trainees accelerate acquisition of these important skills during their training.
Theileria ovis (Piroplasmida: Theileriidae) Detected in Melophagus ovinus (Diptera: Hippoboscoidea) and Ornithodoros lahorensis (Ixodida: Argasidae) Removed From Sheep in Xinjiang, China
Authors: Zhao Li , Wang Jinling , Ding Yulin , Li Kairui , He Bo , Li Fei , Zhang Luyao , Li Xianqiang , Liu Yonghong 
Published: November 2019

Abstract
Theileria spp. are tick-transmitted, intracellular apicomplexan protozoan parasites that infect a wide range of animals and, as such, can cause significant economic losses. The aim of the present study was to detect and analyze apicomplexan parasites from two different ectoparasites that were collected from Xinjiang Uygur Autonomous Region, China. The PCR-based detection of 18S rRNA indicated that Ornithodoros lahorensis specimens from Kashgar, Xinjiang, and Aksu were positive for Theileria spp., as were Melophagus ovinus specimens from Aksu. Meanwhile, phylogenetic analysis, based on the 18S rRNA gene sequences, revealed that the four amplified Theileria sequences could be attributed to T. ovis. To the best of our knowledge, this is the first study to report the detection of T. ovis DNA in M. ovinus and the first molecular identification study to confirm the detection of T. ovis in O. lahorensis in China. Accordingly, the present study extends the known distribution of T. ovis.
Aberrant Accumulation of BRCA1 in Alzheimer Disease and Other Tauopathies
Authors: Nakamura Masataka , Kaneko Satoshi , Dickson Dennis W , Kusaka Hirofumi 
Published: November 2019

Abstract
BRCA1 plays an important roles in several biological events during the DNA damage response (DDR). Recently, some reports have indicated that BRCA1 dysfunction is involved in the pathogenesis of Alzheimer disease (AD). Furthermore, it has also been reported that BRCA1 accumulates within neurofibrillary tangles (NFTs) in the AD brain. In this study, we examined the immunohistochemical distribution of BRCA1 and another DDR protein, p53-Binding Protein 1 (53BP1), in AD, Pick disease (PiD), progressive supranuclear palsy (PSP), corticobasal degeneration, and frontotemporal dementia with parkinsonism linked to chromosome 17. In control subjects, neither BRCA1 nor phosphorylated BRCA1 (pBRCA1; Ser1524) immunoreactivity was observed in neurons or glial cells; and that for pBRCA1 (Ser1423) and 53BP1 were slightly detected in neuronal nuclei. The immunoreactivity for both BRCA1 and pBRCA1 (Ser1423) was localized within phosphorylated tau inclusions in all tauopathies, whereas that for pBRCA1 (Ser1524) was mainly associated with Pick bodies in PiD and to a lesser extent with NFTs in AD. On the other hand, 53BP1-immunoreactive deposits tended to be increased in the nucleus of neurons in AD and PSP compared with those in control cases. Our results suggest that DDR dysfunction due to cytoplasmic sequestration of BRCA1 could be involved in the pathogenesis of tauopathies.
Activation of the Unfolded Protein Response and Proteostasis Disturbance in Parkinsonism-Dementia of Guam
Authors: Verheijen Bert M , Lussier Celina , Müller-Hübers Cora , Garruto Ralph M , Oyanagi Kiyomitsu , Braun Ralf J , van Leeuwen Fred W 
Published: November 2019

Abstract
Guam parkinsonism-dementia (G-PD) is a progressive and fatal neurodegenerative disorder among the native inhabitants of the Mariana Islands that manifests clinically with parkinsonism as well as dementia. Neuropathologically, G-PD is characterized by abundant neurofibrillary tangles composed of hyperphosphorylated tau, marked deposition of transactive response DNA-binding protein 43 kDa (TDP-43), and neuronal loss. The mechanisms that underlie neurodegeneration in G-PD are poorly understood. Here, we report that the unfolded protein response (UPR) is activated in G-PD brains. Specifically, we show that the endoplasmic reticulum (ER) chaperone binding immunoglobulin protein/glucose-regulated protein 78 kDa and phosphorylated (activated) ER stress sensor protein kinase RNA-like ER kinase accumulate in G-PD brains. Furthermore, proteinaceous aggregates in G-PD brains are found to contain several proteins related to the ubiquitin-proteasome system (UPS) and the autophagy pathway, two major mechanisms for intracellular protein degradation. In particular, a mutant ubiquitin (UBB+1), whose presence is a marker for UPS dysfunction, is shown to accumulate in G-PD brains. We demonstrate that UBB+1 is a potent modifier of TDP-43 aggregation and cytotoxicity in vitro. Overall, these data suggest that UPR activation and intracellular proteolytic pathways are intimately connected with the accumulation of aggregated proteins in G-PD.
Feasibility study on a novel tiny dosimeter using a barium titanate capacitor
Authors: Kuga Yuma , Ogawara Ryo , Ishikawa Masayori 
Published: November 2019

ABSTRACT
In our laboratory we have confirmed that the capacitance of barium titanate-based capacitors changes due to radiation. Since a commercially available capacitor is very small and inexpensive, it may be used as a multidimensional dose meter in which a large number of capacitor elements are arranged, or may be embedded in the body and used as an in-vivo dose meter. In this study we examined the usefulness of a dosimeter using the capacitance change of a barium titanate capacitor. As a basic property, it was confirmed that the dose linearity was good. With regard to dose rate characteristics and response to fractionated irradiation, capacitance change due to aging affects measurement accuracy, but online measurement of capacitance change immediately before irradiation can be performed to correct aging effects during irradiation. By doing this, we confirmed that the dose rate characteristics and the response to fractionated radiation are improved.
Investigating the amphiatlantic status of Facelina bostoniensis (Couthouy, 1838) (Nudibranchia: Aeolidida)
Authors: Carmona Leila 
Published: November 2019

ABSTRACTThe aeolid species Facelina bostoniensis (Couthouy, 1838) was originally described from Massachusetts and was later reported from the Eastern Atlantic Ocean and the Mediterranean Sea. So far, no molecular systematic study of its amphiatlantic status has been carried out. Phylogenetic analyses (maximum likelihood and Bayesian) of DNA sequence data for the mitochondrial cytochrome c oxidase subunit I and 16S rRNA genes confirm the amphiatlantic status of F. bostoniensis. My findings show that this species is restricted to the Atlantic realm and that the species recorded from the Mediterranean is not F. bostoniensis but F. vicina (Bergh, 1882). It is hypothesized that previous records of F. bostoniensis from the Mediterranean Sea were actually misidentifications of F. vicina.
Sinorhizobium meliloti YbeY is a zinc-dependent single-strand specific endoribonuclease that plays an important role in 16S ribosomal RNA processing
Authors: Babu Vignesh M P , Sankari Siva , Budnick James A , Caswell Clayton C , Walker Graham C 
Published: November 2019

Abstract
Single-strand specific endoribonuclease YbeY has been shown to play an important role in the processing of the 3′ end of the 16S rRNA in Escherichia coli. Lack of YbeY results in the accumulation of the 17S rRNA precursor. In contrast to a previous report, we show that Sinorhizobium meliloti YbeY exhibits endoribonuclease activity on single-stranded RNA substrate but not on the double-stranded substrate. This study also identifies the previously unknown metal ion involved in YbeY function to be Zn2+ and shows that the activity of YbeY is enhanced when the occupancy of zinc is increased. We have identified a pre-16S rRNA precursor that accumulates in the S. meliloti ΔybeY strain. We also show that ΔybeY mutant of Brucella abortus, a mammalian pathogen, also accumulates a similar pre-16S rRNA. The pre-16S species is longer in alpha-proteobacteria than in gamma-proteobacteria. We demonstrate that the YbeY from E. coli and S. meliloti can reciprocally complement the rRNA processing defect in a ΔybeY mutant of the other organism. These results establish YbeY as a zinc-dependent single-strand specific endoribonuclease that functions in 16S rRNA processing in both alpha- and gamma-proteobacteria.
Characterization of zygotic genome activation-dependent maternal mRNA clearance in mouse
Authors: Sha Qian-Qian , Zhu Ye-Zhang , Li Sen , Jiang Yu , Chen Lu , Sun Xiao-Hong , Shen Li , Ou Xiang-Hong , Fan Heng-Yu 
Published: November 2019

Abstract
An important event of the maternal-to-zygotic transition (MZT) in animal embryos is the elimination of a subset of the maternal transcripts that accumulated during oogenesis. In both invertebrates and vertebrates, a maternally encoded mRNA decay pathway (M-decay) acts before zygotic genome activation (ZGA) while a second pathway, which requires zygotic transcription, subsequently clears additional mRNAs (Z-decay). To date the mechanisms that activate the Z-decay pathway in mammalian early embryos have not been investigated. Here, we identify murine maternal transcripts that are degraded after ZGA and show that inhibition of de novo transcription stabilizes these mRNAs in mouse embryos. We show that YAP1-TEAD4 transcription factor-mediated transcription is essential for Z-decay in mouse embryos and that TEAD4-triggered zygotic expression of terminal uridylyltransferases TUT4 and TUT7 and mRNA 3′-oligouridylation direct Z-decay. Components of the M-decay pathway, including BTG4 and the CCR4-NOT deadenylase, continue to function in Z-decay but require reinforcement from the zygotic factors for timely removal of maternal mRNAs. A long 3′-UTR and active translation confer resistance of Z-decay transcripts to M-decay during oocyte meiotic maturation. The Z-decay pathway is required for mouse embryo development beyond the four-cell stage and contributes to the developmental competence of preimplantation embryos.