bluuebunny/crossref_metadata_2025_split huggingface dataset:
LINC00886, as a Biomarker Indicative of Thyroid Cancer Dedifferentiation, Negatively Regulates the Malignancy in Anaplastic Thyroid Cancer
Authors: Ma Ben , Luo Yi , Xu Weibo , Han Litao , Liu Wanlin , Jiang Hongyi , Wang Xiao , Wen Shishuai , Liao Tian , Wei Wenjun , Ji Qinghai , Wang Yu 
Published: July 2020

Abstract
Backgroud: Anaplastic thyroid cancer (ATC) is the most aggressive type of thyroid cancer. This study aimed to identify a long non-cdoing RNA (lncRNA) as a specific biomarker associated with ATC, and further investigated its biological function and molecular mechanism underlying regulation of malignancy in ATC.Methods: We searched for lncRNAs associated with dedifferentiation and screened out specific lncRNAs significantly deregulated in ATC by using transcriptome data of dedifferentiation cancers from Fudan University Shanghai Cancer Center (FUSCC) and the Gene Expression Omnibus (GEO) database. The above lncRNAs were analyzed to identify a useful biomarker in thyroid cancer patients from the FUSCC, GEO and The Cancer Genome Atlas, which was further investigated for its functional roles and molecular mechanism in ATC in vitro.Results: The clinicopathological association analyses revealed that LINC00886 was correlated with thyroid cancer dedifferentiation and significantly suppressed in ATC. In vitro, LINC00886 was confirmed to negatively regulate cell proliferation, colony formation and cell migration and invasion of ATC. LINC00886 physically interacted with protein kinase R (PKR) and affected its stability through ubiquitin/proteasome-dependent degradation pathway in the ATC cell. Decreased PKR caused by LINC00886 downregulation, enhanced the activity of eukaryotic initiation factor 2α (eIF2α) via reducing the phosphorylation of eIF2α and thus promoted protein synthesis to maintain the ATC malignancy. Conclusions: Our findings identify LINC00886 as a novel biomarker of thyroid cancer and suggest that the LINC00886/PKR/eIF2α signaling axis is a potential therapeutic target in ATC.
Knowledge and attitudes among medical students toward the clinical usage of e-cigarettes: A cross-sectional study in a university hospital in Saudi Arabia
Authors: Alzahrani Sami , Almutairi Ahmed Mabruk , Alghamdi Ali Ahmed , Aljuhani Abdullah Abdulwahab , ALbalawi Abdulrahman Hamed 
Published: July 2020

Abstract
Background

To assess knowledge and attitudes about e-smoking among undergraduate medical students, specifically focused on favorable view of therapeutic ecigarette use for smoking cessation or harm reduction.
Methods

This cross-sectional study included medical students at King Abdulaziz University, Saudi Arabia. A six-item subscale was used to explore knowledge and attitudes about the therapeutic use of e-cigarettes, measuring participants’ likelihood of favoring such use. A four-item questionnaire measured confidence and importance of being educated about smoking and e-smoking, in addition to sources of knowledge about e-cigarettes.
Results

A total of 399 students participated. Smoking history included current smokers (19.8%) and ex-smokers (6.5%), while e-cigarettes were tried by 36.6% and are currently used by 11.5%. A minority (13.5%) believed that e-cigarettes are FDA-approved for smoking cessation, while approximately one-third believed e-smoking lowers cancer risks (31.1%) and could help with smoking cessation (31.1%). Further, 35.9% agreed or strongly agreed that e-cigarettes are better for patients than tobacco products, and 17.5% were likely to recommend e-smoking to their patients for smoking cessation. Reliability of the six-item scale showed Cronbach’s alpha = 0.676, which was enhanced to 0.746 after deletion of one item about addictiveness. Using the corrected five-item scale, 23.6% of the participants would favor therapeutic use of e-cigarettes.
Conclusion

We observed several misconceptions about addictiveness and inadequate awareness about e-cigarettes’ harmful effects, leading to non-scientific opinions about its therapeutic use for harm reduction or in smoking cessation. Academic programs around this topic should be updated in accordance with majority expert recommendations.
Multi-Locational Evaluation of Medium-Staple Cotton Genotypes for Seed-Cotton Yield under the Middleveld Agro-Ecological Zone of Zimbabwe.
Authors: Mare Marco , Chapepa Blessing , Mubvekeri Washington 
Published: July 2020

Abstract
Background The Zimbabwe national cotton breeding programme has the mandate to develop superior cotton (Gossypium Hirsutum) varieties with good field performance and high fibre properties. Cotton productivity in Zimbabwe has remained very low, with national average seed cotton yield record of 650 kg/ha (AMA Report, 2019) compared to the potential 2000 kg/ha. Though this is a result of many biotic and abiotic factors, field experiments laid in a Randomized Complete Block Design were conducted on ten genotypes (seven test genotypes and three check varieties) from 2012 to 2019 across 13 diverse locations in Zimbabwe to evaluate cotton yield performance, stability and adaptability by Analysis of Variance (ANOVA) and Genotype and Genotype by Environment Interaction (GGE) Biplot methods.Results The Analysis of Variance indicated significant (P < .001) effects of Genotype (G), Environment (E) and their Interaction (GE). The highest percentage of variation was explained by E/G/GE (60.34%) while G/E + GE together explained the rest of the variation (< 40%). Joint effects of G and GE were partitioned using the GGE biplot analysis explaining total of 59.08% (PC1 = 36.96% and PC2 = 22.12%) of the GGE sum of squares. The biplot analysis revealed that candidates 917-05-7, TN96-05-9, 912-05-1 and GN 96 (b)-05-8 were the ideal and stable genotypes. The candidate variety 917-05-7 significantly (P < .001) showed superior yield performance over checks CRI-MS1 and CRI-MS2 recording 5% and 5.5% yield increase respectively. Candidate 917-05-7 recorded a higher earliness index (78.11%) over checks CRI-MS1 and CRI-MS2 (77 and 76% respectively) thus indicating potential attributes for good cotton production with more pick-able bolls earlier than the current commercial varieties.Conclusion Candidate 917-05-7 has been identified as the ideal genotype in terms of high yielding potential, and stability hence recommended for commercial release and use as breeding parent for future breeding programs.
Adverse Impact of Climate Change on Mambioko Community Dependent Forest and Local Perception on Vulnerabilities and Responses-Centre Region of Cameroon
Authors: Nghogekeh Tieminie Robinson , Eugene Chia Loh , Mandiefe Piabuo Serge , Mayiadieh Nghobuoche Frankline 
Published: July 2020

Abstract
This study was undertaken to identify and assess climate change vulnerabilities and impacts on the livelihoods of forest dependent communities in Mambioko community forest in the Centre Region of Cameroon. Primary data was collected from 120 households; key informants, focus group discussions and was supplemented with secondary data from desk review of relevant literature. Forest resources were generally perceived by forest dependent communities as declining. Climate Change was observed to have impacted on their major livelihood’s activities/resources of forest dependent communities by way of prolonged drought, erratic rainfall pattern, and variability in rainfall intensity. These trends have consequently led to drying of rivers and streams, shift in cropping season, reduced yield, frequent and extreme warm conditions among others. Use of irrigation, crop diversification, household income diversification through alternative livelihood activities and increase in size of cultivated farms are among the different measures being adopted by communities to cope up with the effects of climate change. Using socio-economic indicators, the bimodal agro ecological zone is vulnerable to climate change impact. The zone has relatively higher dependency on climate sensitive livelihood activities/resources, limited diversified income sources and moderate access to climate change information. In an attempt to help forest dependent communities cope up with climate change impacts, fundamental issues such as non-climate dependent alternative livelihood activities, access to climate, sustainable agricultural practices and sustainable forest resources management are key areas for various multidisciplinary intervention.
The Sulfatide/Myb/MEF2C Axis Controls the Malignant Behaviors of Hepatocellular Carcinoma Cells by Modulating lncAY Expression
Authors: Chen Mei Hua , Qi Bing , Cai Qian Qian , Sun Jia Wen , Fu Li Sheng , Kang Chun Lan , Ma Ming Zhe , Wu Xing Zhong 
Published: July 2020

Abstract
The authors have requested that this preprint be removed from Research Square.
Evaluation of Implementation and Effectiveness of a Pilot Multi-Domain Program for Older Adults at Risk of Cognitive Impairment at Neighborhood Senior Centres – A Randomized Controlled Trial
Authors: Ng Pei Ern Mary , Nicholas Sean Olivia , Wee Shiou-Liang , Yau Teng Yan , Chan Alvin , Chng Isaiah , Yap Lin Kiat Philip , Ng Tze Pin 
Published: July 2020

Abstract
Background The efficacy of multi-domain interventions for dementia prevention has been investigated in a few large randomized controlled trials (RCT). To link research to community action, we evaluated the implementation and effectiveness of a community-based multi-domain program for older adults at risk of cognitive impairment. Methods Three enterprises implemented a 24-week, bi-weekly, multi-domain program for older adults at risk of cognitive impairment through neighborhood senior centres (SCs). The program comprised a combination of dual-task group physical exercise, pen-and-paper cognitive games, computerised cognitive training, and mobile application-based personalized nutritional guidance. An effectiveness-implementation hybrid design using a RCT design and informed by the RE-AIM (Reach, Effectiveness, Adoption, Implementation, Maintenance) framework was adopted to evaluate the program. Cognition and quality of life were assessed using the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS), and EuroQol EQ-5D-5L (EQ-5D) respectively, at baseline and 24-weeks. Blood lipid panel and physical assessments were also conducted. Questionnaires on implementation outcomes were administered at the participants-, provider- and community-levels to participants, implementers, and SC managers. Program schedules and attendance were obtained from implementers and the research team conducted unannounced, random and non-intrusive observations of the program. Results The intervention program reached almost 50% of eligible participants, had an attrition rate of 22%, and was adopted by 8.7% of the SCs approached. The intervention was implemented as intended, except the nutritional component that was re-designed due to participants’ unfamiliarity with the application. There were no between-group differences in cognition, quality of life, and blood lipid panel, though there was a reduction in quality of life in the control group and improved physical function in the intervention group at 24-weeks. Intervention group participants reported improvement in their physical and cognitive functions. The program was well-received by participants and centre managers, who wanted the intervention to continue post-study. Conclusions A six-month multi-domain program for older adults at risk of cognitive impairment can be implemented through neighborhood SCs and elicit improvement in physical but not cognitive performance. Implementation evaluation highlight areas to improve on the uptake of such community-based interventions. Trial registration Trial registration: ClinicalTrials.gov NCT04440969. Registered 22 June 2020 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT04440969
Characteristics of mandibular third molars that have greater risk to cause pathologies
Authors: Ye Zhouxi , Qian Wenhao , Yang Chi , Wu Yubo , Sun Bing , Li Zhiyao , Ling Feng , Xiang Fei , Zhang Yu , Zhu Minwen , Li Hongquan 
Published: July 2020

Abstract
Background

To evaluate the associations of impaction patterns of mandibular third molars (M3Ms) with pathologies caused by them.
Methods

In this study, 262 patients with 432 impacted M3Ms were included. The pathologies include pericoronitis, mandibular second molar (M2M) caries, and M2M distal periodontal pathology. The impaction patterns of M3Ms and the pathologies were examined, and the M2Ms outcomes after the surgeries were evaluated. χ2 test was used to analyze the data and a P value of < 0.05 was considered statistically significant.
Results

Pericoronitis was the major symptom in all patients, whereas the propensities of M2M distal caries and periodontal pathologies increased in older patients. Soft tissue impacted and vertically angulated teeth were more associated with the pericoronitis (p < 0.05); Mesio-angular impacted teeth in less deep positions had greater risks of M2Ms distal caries (p < 0.05); Mesio-angular and horizontal impacted teeth in relative deep positions were more likely to cause M2Ms distal periodontal pathologies (p < 0.05).
Conclusions

Extractions of soft tissue impacted teeth in vertical angulations should be considered. While removals of mesially and horizontally angulated or bony impacted teeth could be delayed.
Factors Associated with Blood Pressure Checkup During Pregnancy Among Women of Reproductive Age in Tanzania; An Analysis of Data from the 2015-16 Tanzania Demographic and Health Survey and Malaria Indicators Survey
Authors: Moshi Fabiola Vincent , Tungaraza Maximilian 
Published: July 2020

Abstract
Background: Hypertensive Disorder of Pregnancy (HDP) is one of the leading causes of maternal mortality and morbidity amongst pregnant women in the world. Blood pressure checkups during pregnancy are on of strategies to identify hypertensive disorders and hence timely management. Little is known on factors associated with blood pressure checkups in Tanzania.Method: The study used data from the 2015-16 Tanzania Demographic and Health Survey and Malaria Indicators Survey (2015-16 TDHS-MIS). A total of 6924women of active reproductive age from 15 to 49 were included in the analysis. Both univariate and multiple regression analyses were used to determine the association between early antenatal booking and maternal services utilization.Results: A total of 4997(72.17%) of interviewed women were checked for blood pressure at least once. After adjusted for the confounders, factors which influenced uptake of blood pressure checkups during pregnancy were timing for antenatal booking within first 12 weeks, AOR=1.496 at 95% CI= 1.297-1.726, p<0.001, age group [ more than 34 years, (AOR=1.518 at 95% CI=1.149-2.006,p=0.003)], wealth index [ middle income, (AOR=1.215 at 95% CI=1.053-1.468, p=0.008) and rich, (AOR=2.270 at 95% CI=1.907-2.702, p<0.001)] reference population being poor; education level [ primary education, (AOR=1.275 at 95%CI=1.107-1.468, p=0.001); secondary, (AOR=2.163 at 95% CI=1.688-2.774, p<0.001) and higher, (AOR= 9.929 at 95%CI=1.355-72.76, p=0.024)] reference population being no formal education; parity[para 2-4, (AOR=1.190 at 95% CI=1.003-1.412, p=0.046) and zones [Unguja Island, (AOR=3.934 at 95% CI=1.568-9.871, p=0.004) and Pemba Island, (AOR=5.308 at 95%CI=1.808-15.58, p=0.002)] Mainland urban being the reference populationConclusion: Blood pressure checkups during pregnancy offer the opportunity for early detection timely management of HDP. The study revealed that rural dwelling pregnant women had higher chance not to get their BP checked. It was also revealed that maternal age, education level, place of residence, wealth index and timing of ANC services significantly associated with blood pressure check-ups. The study identifies the need to explore significant factors associated with utilization of the available free reproductive health services across all public health facilities and to address prioritized intensive awareness programs and behavioral change interventions on the significance of BP checkups among pregnant women of reproductive age.
A Rational Framework for Development Iranian Twin Registry: Lessons Learned from Developed Twin Registries
Authors: Abtahi Hamidreza , Gholamzadeh Marsa , Shahmoradi Leila , Shariat Mamak , Zendehdel Kazem 
Published: July 2020

Abstract
Background: Regarding technology advancement in health sciences and the possibility of recording and organizing a huge amount of data, registries in various clinical domains have been established. The specific genetic structure of twins has enabled researchers to find answers to role of genetics and environment in medical sciences. Thus, twin registries were developed across the world to support twin studies. The main objective of this study is devising a conceptual model for developing the Iranian twin registry to ensure the success of this registry. Methods: In this descriptive and qualitative study, the combination of literature review, brainstorming, and focus group discussions were applied to develop a conceptual model of the twin registry. Based on our qualitative and thematic analysis, the workflow of information gathering and different data levels are described in this study. Results: According to the characteristics of established worldwide twin registries, the success factors in registry implementation were recognized. Moreover, based on our objectives, the hierarchical conceptual model of the registry was invented. The source of information, the different levels of data, and the information flow were determined based on this model. The workflow modeling of data gathering was represented through this survey to clarify the data collection and saving processes. Conclusion: Suggesting a conceptual model and standard framework for twin registry development at the national level based on the experiences of other countries could guide researchers in twin registries implementation efficiently. Moreover, the next steps of implementing a twin registry in a standardized and stepwise way are outlined in this project.
The Effect and Mechanism of ROS-Mediated Activation of NLRP3 Inflammasome in Hepatic Alveolar Echinococcosis
Authors: chen caisong , zhang yaogang , wang zhixing , wang haijiu , fan haining 
Published: July 2020

Abstract
Background: The NLRP3 inflammasome is a significant component of the innate immune system that plays a vital role in the development of various parasitic diseases. However, its role in hepatic alveolar echinococcosis (HAE) remains unclear. Therefore, we aimed to investigate the NLRP3 inflammasome and its activation mechanism in HAE.Method: We assessed the expression of NLRP3, Caspase-1, interleukin (IL)-1β, and IL-18 in the inflammatory marginal zone and corresponding normal liver tissues of 60 patients with HAE. We used a rat model of HAE to investigate the role of the NLRP3 inflammasome in the inflammatory marginal zone of HAE. Transwell experiments were conducted to investigate the effect of Echinococcus multilocularis in stimulating Kupffer cells and hepatocytes. Further, immunohistochemical staining, western blotting, and ELISA assessed the expression of NLRP3 and Caspase-1, IL-1β, and IL-18, and flow cytometry was used to detect apoptosis and ROS.Results: The activation of NLRP3 inflammasome was intimately associated with the expression of ROS(P<0.05). Inhibition of ROS generation decreased the activation of NLRP3-Caspase-1-IL-1β pathway and mitigated damage to hepatocytes (P <0.01) as well as inflammation (P <0.001) in HAEConclusion: E. multilocularis can induce hepatocytes damage and inflammatory response by activating the ROS-NLRP3-Caspase-1-IL-1β pathway in Kupffer cells, indicating that ROS may be a potential target for the treatment of HAE.
Clinical Benefit and Agenda for Clinical Sequencing Using Cancer Panel Testing: A Retrospective Clinical Study
Authors: Nishimura Sadaaki , Sugimoto Atsushi , Kushiyama Shuhei , Togano Shingo , Kuroda Kenji , Yamauchi Makoto , Sumi Toshiyuki , Kaneda Hiroyasu , Kawaguchi Tomoya , Kato Minoru , Kimura Kenjiro , Nagahara Hisashi , Lee Shigeru , Muguruma Kazuya , Takashima Tsutomu , Kubo Shoji , Ohira Masaichi , Yashiro Masakazu 
Published: July 2020

Abstract
Background: Clinical sequencing using a multiplex gene panel has recently been applied worldwide for patients with refractory solid tumors, but the significance of clinical sequencing using multiplex gene panel testing remains uncertain. Here we sought to clarify the feasibility and utility of clinical sequencing in the treatment of refractory tumors at our hospitalMethods: A total of 28 patients with advanced solid tumors treated at our hospital between 2018 and 2020 were enrolled in the clinical sequencing. Among them, we identified 26 patients whose tissue samples were of suitable quality for clinical sequencing, and we analyzed the genomic profiles of these tumors.Results: Pathogenic alterations were detected in 20 (77%) of the 26 patients. The most common mutation was TP53 (6/26, 35%), followed by KRAS (5/26, 19%), and the highest frequency of gene amplification was ERBB2 (5/26, 19%). Five of the 26 patients were identified as candidates for novel molecular-targeted therapy based on their actionable gene alterations, but none of the cases had an advantage for novel targeted therapy following the genetic tests; the reasons were clinical deterioration, refusal for off-label use, and complexity of clinical trial access.Conclusions: Our current results suggested that clinical sequencing might be useful for the detection of pathogenic alterations and the management of additional cancer treatment. Both improved optimal timing of clinical sequencing and a consensus about its off-label use might help patients receive greater benefit from clinical sequencing.Trial registration: This study was performed as a part of clinical study approved by Ethical Committee of Osaka City University Graduate School of Medicine (Permission number: 4199, 3925)
miR-181&nbsp;Expression is Associated With The Prognosis in Lung Cancer.
Authors: Zhao Yue , Kong Xiangjun , Wang Hongbing 
Published: July 2020

Abstract
Background: Lung cancer is one of the most common cancers, with high morbidity and mortality. MiRNAs are proved to play important roles in various human cancers. In our study, we aimed to explore the prognostic value of miR-181 in lung cancerMethods: Quantitative real-time polymerase chain reaction (QRT-PCR) was used to detect the expression level of miR-181 in lung cancer tissues and the paired non-cancerous tissues. The relationship between miR-181 expression and clinicopathologic parameters were analyzed by chi-square test. Kaplan-Meier method with log rank test was applied for overall survival analysis. Furthermore, the Cox regression analyses were performed to evaluate the prognostic value of miR-181 in lung cancer.Results: Down-regulated miR-181 expression was observed in lung cancer tissues (P<0.001), moreover, its expression was significantly correlated with TNM stage (P=0.015) and metastasis (P=0.000). In addition, lung cancer patients with lower miR-181 expression level had poorer overall survival than those with higher expression (log rank test, P=0.011). Cox regression analysis suggested that miR-181 was an independent prognostic factor for lung cancer (HR=1.961, 95%CI=1.135-3.388, P=0.016).Conclusion: MiR-181 may be a tumor suppressor gene in lung cancer, which can predict outcomes for the patients.