bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-omics Prognostic Analysis of Lysine Acetylation Regulators in Glioma
Authors: Tu Zewei , Lv Shigang , Wu Lei , Hu Qing , Tao Chuming , Li Kuangxun , Zhu Xingen , Huang Kai 
Published: June 2020

Abstract
Background: Lysine acetylation is a crucial kind of protein modification and is related to the malignant development of various cancers. But their roles in glioma are still unclear and needed concluded comprehensively. Methods: In this study, we comprehensively analyzed the expression levels of 33 lysine acetylation regulators (LARs) and prognostic roles by using public data, including the Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA). The prognostic roles of LARs were judged by univariate Cox regression. Consensus clustering was applied to result in three stratified glioma subtypes (LA1, 2, and 3) with different clinical outcomes. We also constructed a risk signature for predicting the overall survival of glioma patients by using least absolute shrinkage and selection operator regression (LASSO regression). Besides, copy number variations (CNVs) and single nucleotide polymorphism (SNP) of LARs were also analyzed in our study. Results: We found the mRNA expression levels of most of LARs were dysregulated in gliomas and associated with the prognosis of glioma patients. The risk signature constructed by 14 LARs presented an independent prognostic role in both the CGGA (HR:1.96, 95%CI:1.33-2.90) and TCGA (HR:1.48, 95%CI:1.08-2.03) datasets and robust predictive effects in the ROC curves with all of area under curves more than 0.800. Moreover, the copy number variations of LARs were also significantly related to the prognosis of glioma patients, in which HDAC1 (1p) was one of the oncogenes lost in 1p/19q codeletion events, while SIRT2 (19q) and EP300 (22q) may act as tumor suppressors in gliomas with 19q or 22q deletions, respectively. Conclusion: LARs are potential biomarkers for the malignant progression of gliomas, and our results could be useful for predicting the OS of glioma patients and provide some clues in searching the functions of LARs in glioma progression. Keywords: glioma, lysine acetylation regulator, epigenetic, prognostic signature, biomarker.
Identification and the First Record of Marine bacteria Mudskippers Boleophthalmus dussumieri (Valenciennes, 1837) by 16S rRNA on the Northern Intertidal areas of Persian Gulf, Iran
Authors: nassirabady nerssy , Ghotbeddin N. Negar , Roomiani Laleh 
Published: June 2020

Abstract
Background

Mudskipper is from the Gobiidae family and from the subfamily Oxudercinae. Mudskipper contains four main genera Boleophthalmus, Periophthalmodon, Periophthalmus, and Scartelaos. The aim of this research identification of bacterial agent (Marine bacteria) mudskippers Boleophthalmus dussumieri on the Shores of the northern intertidal areas of the Persian Gulf (Abadan, Hendijan and Bahrekan), Iran. The mud sediment and water samples (at the depth of one m), and mudskipper fish samples (near shore at the intertidal) were collected from Abadan, Hendijan and Bahrekan in northern coasts of the Persian Gulf, Iran.
Results

A total of 80 aerobic bacteria marine (from the intestines of mudskipper, seawater, and muddy sediments ) was identified by biochemical tests and 16S rRNA gene sequence analysis. The bacteria's (Marine bacteria) [Eubacterium] tenue strain DSM 20695, Praclostridium bifermentans strain JCM 1386 and Vibrio hyugaensis strain 090810a was first identified in the northern intertidal areas of Persian Gulf, Iran.
Conclusions

Bacteria compete with other organisms to get oxygen to the surface layers of the flower bed. They give that the bacterial species common to the fish intestines and sediments is high and due to fish feeding of sediment it can be concluded that the species present in the fish intestine in the present study may be a function of substrate species that needs further studies to prove this.
Microscopic extra-laminar sequestrectomy (MELS) for the treatment of hidden zone lumbar disc herniation: report of the surgical technique, patient selection, and clinical outcomes
Authors: Wang Chunxiao , Zhang Yao , Tang Xiaojie , Cao Haifei , Song Qinyong , Tan Jiangwei , Jin Chengzhen , Song Hongkai 
Published: June 2020

Abstract
Background

Surgical management of lumbar disc herniation in the hidden zone is technically challenging due to its difficult surgical exposure. The conventional interlaminar approach harbors the potential risk of post-surgical instability, while other approaches consist of complicated procedures with a steep learning curve and prolonged operation time.
Objective

To introduce a safe and effective technique named microscopic extra-laminar sequestrectomy (MELS) for treatment of hidden zone lumbar disc herniation and present clinical outcomes within a two year follow-up period.
Methods

Between Jan 2016 to Jan 2018, twenty one patients (13 males) with hidden zone lumbar disc herniation were enrolled in this study. All patients underwent MELS (19 patients underwent sequestrectomy only, 2 patients underwent an additional inferior discectomy). The nerve root and herniated fragment were visually exposed using this extra-laminar approach. The operation duration, blood loss, intra- and postoperative complications, and recurrences were recorded. The Visual Analog Scale (VAS), Oswestry Disability Index (ODI), and the modified MacNab criteria were used to evaluate clinical outcomes. Postoperative stability was evaluated both radiologically and clinically.
Results

The mean follow-up period was 20.95 ± 2.09 months, ranging from 18 to 24 months. The mean operation time was 32.43 ± 7.19 min and the mean blood loss was 25.52 ± 5.37 ml. All patients showed complete neurological symptom relief after surgery. The VAS and ODI score were significantly improved at the final follow-up compared to those before operation (7.88 ± 0.70 vs 0.10 ± 0.30, 59.24 ± 10.83 vs 11.29 ± 3.59, respectively, p < 0.05). Seventeen patients (81%) obtained an “excellent” outcome and the remaining four (19%) patients obtained a “good” outcome based the MacNab criteria. One patient suffered reherniation at the same level one year after the initial surgery and underwent a transforaminal endoscopic discectomy. No major complications and postoperative instability were observed.
Conclusions

Our observation suggest that MELS is a safe and effective method in the management of hidden zone lumbar disc herniation. Due to its relative simplicity, it comprises a flat surgical learning curve and shorter operation duration, and overall results in reduced disturbance to lumbar stability.
ABBV-744 and Onalespid as Potential Inhibitors of SARS-CoV-2 main Protease Enzyme: A Promising Therapeutics in COVID-19?
Authors: Fakhar Zeynab , Khan Shama , Ahmad Aijaz 
Published: June 2020

Abstract
A new pathogen severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide and become pandemic with thousands new deaths and infected cases globally. To address coronavirus disease (COVID-19), currently no effective drug or vaccine is available. This necessity motivated us to explore potential lead compounds by considering drug repurposing approach targeting main protease (Mpro) enzyme of SARS-CoV-2. This enzyme considered to be an attractive drug target as it contributes significantly in mediating viral replication and transcription. Herein, comprehensive computational investigations were performed to identify potential inhibitors of SARS-CoV-2 Mpro enzyme. The structure-based pharmacophore modeling was developed based on the co-crystallized structure of the enzyme with its biological active inhibitor. The generated hypotheses were applied for virtual screening based PhaseScore. Docking based virtual screening work-flow was used to generate hit compounds using HTVS, SP and XP based Glide GScore. The pharmacological and physicochemical properties of the best hit compounds were characterized using ADMET. Molecular dynamics simulations were performed to explore the binding affinities of the considered compounds. Binding studies revealed that compound ABBV-744 binds to the Mpro with strong affinity (Gbind -45.43 kcal/mol), and the complex is more stable in comparison with other protein-ligand complexes. Our study classified three best compounds which could be considered as promising inhibitors against main protease SARS-CoV-2 virus.
Educational Intervention for Increasing Teachers’ ADHD Knowledge, Attitude, and Behavior
Authors: hossennia Maede , Mazaheri Maryam Amidi , Heidari Zahra 
Published: June 2020

Abstract
Background: this study evaluated the effect of an educational intervention for increasing elementary school teachers’ ADHD knowledge, attitudes, and behavior.Method: Teachers from 12 schools who had at least one students with a definitive diagnosis of ADHD participated in the study and were allocated into either an intervention (n=31) or control group (n=27). Teachers’ ADHD knowledge, Attitude, and Behavior were assessed before and after intervention. Six-session participatory intervention were provided only for the intervention group.Results: Two mounts after the intervention, the intervention group scored significantly higher on all three outcomes (P≤0.001).Conclusion: The educational intervention significantly improved teachers’, knowledge, attitudes as well as their behaviors and strategies to manage children with ADHD. It is recommended that this program will incorporate into in-service training courses for primary school teachers as a means of facilitating teaching and managing children with ADHD in class. Trial registration: The study was approved by the by the Research Deputy of Isfahan University of Medical Sciences. In addition, The Ethical Committee of Isfahan University of Medical Sciences approved the study proposal. (ID code: IR.MUI.RESEARCH.REC.1398.297). The required permission from Education Department of Isfahan City was attained. Participation in the study was voluntary. Before taking part in the study, selected teachers provided written consent also study goals were described to them.
Comorbidity Accrual and Mortality Rate for Patients with Giant-Cell Arteritis and Polymyalgia Rheumatica are Similar Regardless of Glucocorticoid Cumulative Dose: A Longitudinal, Retrospective Study
Authors: Pokroy-Shapira Elisheva , Dortort-Lazar Ariela , Molad Yair 
Published: June 2020

Abstract
Background: Patients with giant-cell arteritis (GCA) and polymyalgia rheumatica (PMR) are treated with high cumulative glucocorticoid (GC) doses during their disease course. We sought to determine the accrual of comorbidity as well as survival in patients with GCA and PMR.Methods: We conducted a retrospective follow-up study of an inception cohort of patients diagnosed with GCA and PMR, persistently followed at our rheumatology clinic during January 1990 - March 2018. Disease manifestations, duration of GC dose tapering and discontinuation, and mortality rate and causes were compiled. Cumulative GC dose was determined. We scored baseline and last encounter age-adjusted Charlson Comorbidity Index (CCI), Rheumatic Disease Comorbidity Index (RDCI), and CCI and RDCI increment (Δ) at last study encounter.Results: The cohort consisted of 69 patients (24 GCA and 45 PMR, 68.1% female). Prednisone cumulative dose was 13,382.19±6189.21 mg and 6,610.36±4,755.55 mg for GCA and PMR, respectively, p <0.001. Prednisone-free remission rate for the entire cohort was 28.9 percent for a mean follow-up period of 7.7 (range: 1-16. 9) years. The CCI and RDCI scores at diagnosis and at last study encounter did not differ between the GCA and PMR groups. Interestingly, initial prednisone dose, time to achieving 50% and 25% of initial prednisone dose, time to daily prednisone dose of 7.5mg and 5mg, as well as time to prednisone discontinuation and prednisone-free survival did not differ when comparing ΔCCI ≥1 and RDCI ≥1 with ΔCCI=0 and RDCI =0, respectively. Mortality rate did not differ between the GCA and PMR groups.Conclusions: Despite significant higher cumulative doses of prednisone for patients with GCA compared to patients with PMR, comorbidity scores and mortality rate were comparable. Our results emphasize the unmet need for novel steroid-sparing drugs in PMR as well as in GCA.Trial registration: This article reports a retrospective study and does not report results of health care intervention.
DPP4 Polymorphisms Are Associated With Hypoalphalipoproteine mia and DPP4 Serum Levels. A Case-control Study of the GEA Cohort.
Authors: Vargas-Alarcón Gilberto , González-Salazar María del Carmen , Vázquez-Vázquez Christian , Couder Adrián Hernández-Díaz , Sánchez-Muñoz Fausto , Reyes-Barrera Juan , Criales-Vera Sergio A. , Sánchez-Guerra Marco , Osorio-Yáñez Citlalli , Posadas-Sanchez Rosalinda 
Published: June 2020

Abstract
Background: Dipeptidyl peptidase-4 (DPP4) can influence lipid homeostasis and atherosclerosis progression. Our aim was to assess the association of DPP4 gene polymorphisms with hypoalphalipoproteinemia and DPP4 levels, in a cohort of Mexican individuals.Methods: Five DPP4 polymorphisms (rs12617336, rs12617656, rs1558957, rs3788979, rs17574) were genotyped in 748 participants with and 745 without hypoalphalipoproteinemia. The associations were evaluated using logistic regression analyses.Results: Under inheritance models adjusted for confounding variables, the rs12617336 (OR = 0.22, Pheterozygote = 0.001) and rs17574 (OR = 0.79, Padditive = 0.033; OR = 0.75, Pdominant = 0.021; OR = 0.75, Pheterozygote = 0.029; OR = 0.74, Pcodominant1 = 0.024) polymorphisms were associated with a low risk of hypoalphalipoproteinemia. Moreover, GTTCG haplotype was also related with a low risk of this condition (OR = 0.75, P = 0.021). Additionally, both polymorphisms were associated with protection for the presence of insulin resistance (IR) (OR = 0.17, Pheterozygote = 0.019 for rs12617336 and OR = 0.75, Padditive = 0.049 for rs17574). The rs12617336 was also associated with a low risk of hyperinsulinemia (OR = 0.11, Pheterozygote = 0.006). Difference in DPP4 levels were observed in individuals with rs17574 genotypes, the rs17574GG genotype individuals had the lowest levels.Conclusions: Our data suggest that rs12617336 and rs17574 DPP4 polymorphism could be envisaged as protective genetic markers for hypoalphalipoproteinemia, IR, and hyperinsulinemia. The rs17574GG genotype was associated with the lowest DPP4 levels.
The Effect of Smoking on Progression From Ductal Carcinoma in Situ to Invasive Ductal Carcinoma: A Retrospective Study
Authors: Takada Koji , Kashiwagi Shinichiro , Asano Yuka , Goto Wataru , Kouhashi Rika , Yabumoto Akimichi , Morisaki Tamami , Shibutani Masatsune , Takashima Tsutomu , Fujita Hisakazu , Hirakawa Kosei , Ohira Masaichi 
Published: June 2020

Abstract
Background:If ductal carcinoma in situ (DCIS) is diagnosed by histological examination such as core needle biopsy or vacuum-assisted biopsy (VAB), invasion is often found by removing the entire tumor and performing pathological examination. According to a meta-analysis, the rate of invasion found by postoperative pathological examination is about 25%. Smoking is a risk factor for carcinogenesis in various carcinomas, including breast cancer. We examined the correlation between the risk of invasion found by postoperative pathology and smoking history in patients who were diagnosed with DCIS by preoperative biopsy.Methods:In this study, we examined 128 patients who were diagnosed with DCIS by preoperative biopsy. Before the biopsy, all patients were asked about the duration of smoking, number of cigarettes smoked per day, and whether they were currently smoking. The data were used to calculate each patient’s tobacco exposure based on pack-years. The statistical analyses included the Pearson’s chi-square test and logistic analysis. Multivariate analysis was performed on the risk factors for invasion diagnosed by postoperative pathological examination in all cases diagnosed with DCIS by preoperative biopsy.Results: Of all the patients included in this study, 107 (83.5%)neversmoked, which represented the majority, while8 patients (6.3%) smoked at diagnosis with DCIS, and 13 (10.2%) had quit smokingbefore diagnosis.Tobacco exposure was 10 or less pack-years for11 patients (8.6%) and more than 10 pack-years for10 patients (7.8%). Number of pack-years was not an independent factor (p=0.349, odds ratio [OR]=0.329), but current smoker status (p=0.006, OR=not calculable) was an independent factor with VAB (p=0.018, OR=0.327).Conclusions: This study suggests that tobacco components may have an influence on the progression from DCIS to invasive ductal carcinoma.
Cluster Analysis of Myelin Nerve Fibers of the Periferal Nerve
Authors: Yurakh Omelian , Popadynets Oksana , Yurakh Halyna , Osypchuk Mykhailo , Tokaryk Nadiya , Hryshchuk Mariia , Kotyk Taras 
Published: June 2020

One of the unsolved issues in neuromorphology is the classification of myelin nerve fibers (MNF). Objective: to use cluster analysis to classify the sciatic nerve MNF.
Material and methods. The work was performed using 5 one-year-old male Wistar rats. Semi-thin sections were stained with methylene blue. MNF morphometry was performed using ImageJ, and statistical processing – using the software environment R.
Results of the study. Ward’s and k-means methods were used to cluster the MNF. Three clusters of MNFs are defined and their parameters are determined. The presented algorithm for adapting the literature data to the format of the obtained results includes determining the total average for the combined set of each indicator and the total variance, which is the sum of intragroup and intergroup variances.
Conclusions: 1) for the classification of MNF it is advisable to use cluster analysis; 2) clustering should be performed according to the transsection areas of the axial cylinder and myelin sheath; 3) the number of clusters is determined by the agglomerative method of Ward, and their metrics – by the iterative method of k-means; 4) three clusters of MNF of the rat sciatic nerve differ in the transsection areas of the fibers, the axial cylinder and the myelin sheath and the percentage of nerve fibers; 5) when comparing identical indicators according to the obtained and literature data, the results were equivalent in the areas of the axial cylinder and myelin sheath and their shape coefficients, despite the fact that the classification of myelin fibers and their morphometry was performed using different methods.
Reparação de danos extrapatrimoniais sofridos pelos familiares de trabalhadores falecidos como consequência de acidente de trabalho
Authors: Almeida Cléber Lúcio de 
Published: June 2020

RESUMO:O presente ensaio examina a tarifação da reparação dos danos extrapatrimoniais decorrentes da relação de trabalho promovida pela Lei n. 13.467/17, utilizando como métodos a revisão bibliográfica e a análise da citada lei à luz da Constituição da República e do Direito Internacional dos Direitos Humanos. Ao final, o que se pretende é apresentar resposta para duas indagações principais: a tarifação da reparação dos danos extrapatrimoniais decorrentes da relação de trabalho promovida pela Lei n. 13.467/17 alcança os familiares dos trabalhadores falecidos em consequência de acidente de trabalho? A tarifação da reparação dos danos extrapatrimoniais decorrentes da relação de trabalho promovida pela Lei n. 13.467/17 é constitucional e está em harmonia com o Direito Internacional dos Direitos Humanos? ABSTRACT:This essay examines the assessment of the reparation of non-property damages resulting from the employment relationship promoted by Statute no. 13.467/17, using as methods bibliographical revision and the analysis of the Statute in light of the Constitution of the Republic and of International Human Rights Law. In the end, it is intended to answer two main questions: Does the assessment of the reparation of non-property damages resulting from the employment relationship promoted by Statute no. 13.467/17 covers the relatives of deceased workers as a result of an accident at work? Is the assessment of the reparation of property damages resulting from the employment relationship promoted by Statute no. 13.467/17 constitutional, and is it in harmony with International Human Rights Law?
Do juiz herói ao tribunal democrático
Authors: Diógenes Beatriz Nunes , Espíndola Diane 
Published: June 2020

RESUMO:O objetivo deste artigo foi refletir sobre o atual papel do Poder Judiciário como porta-voz do texto constitucional e ator político em constante relação conflituosa com o legislativo. Discutiu-se sobre o ativismo judicial e a atuação do Supremo Tribunal Federal como legislador moral (superego da sociedade), a ideia do juiz herói e seus desdobramentos democráticos, principalmente quanto ao sistema de freios e contrapesos. Observou-se a existência de um movimento de hiperjudicialização de questões éticas e políticas, que acarreta o desequilíbrio da dinâmica institucional do modelo democrático. Conclui-se que a atuação do Poder Judiciário não deve ser marcada pelo decisionismo disfarçado de ordem de valores, nem tampouco deve atuar como legislador moral e nem permitir a ausência de vinculação ao padrão normativo. Foi sugerido, para tanto, o aperfeiçoamento da dinâmica institucional brasileira através da interação permanente entre o judiciário e o legislativo, em busca da formação de uma teoria normativa da separação de poderes que promova um debate qualitativo consubstanciado na razão pública, que resguarde direitos e tonifique a dimensão deliberativa do modelo político em vigor. Utilizou-se, para tal reflexão, do método de pesquisa bibliográfico.ABSTRACT:The purpose of this article was to reflect on the current role of the judiciary as a spokesperson for the constitutional text and political actor in constant conflicting relationship with the legislative. It was discussed about judicial activism and the performance of the Supreme Court as moral legislator (society’s superego), the idea of the judge hero and its democratic repercussions, especially regarding the system of checks and balances. The existence of a movement of hyperjudicialization of ethical and political issues was observed, which causes the imbalance of the institutional dynamics of the democratic model. It is concluded that the performance of the judiciary should not be marked by decisionism disguised as an order of values, nor should it act as a moral legislator or allow the absence of binding to the normative standard. To this end, it was suggested that the Brazilian institutional dynamics be improved through the permanent interaction between the judiciary and the legislature, seeking the formation of a normative theory of the separation of powers that promotes a qualitative debate embodied in public reason that safeguards rights and tones the deliberative dimension of the current political model. For such reflection, the bibliographic research method was used