bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of mitochondrial function‐associated lncRNAs in septic mice myocardium
Authors: Shi Yingzhou , Zheng Xiaohe , Zheng Mingzhi , Wang Linlin , Chen Yingying , Shen Yueliang 
Published: August 2020

AbstractThe present study aimed to analyze long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in septic mice heart and to identify potential lncRNAs and mRNAs that be responsible for cardiac mitochondrial dysfunction during sepsis. Mice were treated with 10 mg/kg of lipopolysaccharides to induce sepsis. LncRNAs and mRNAs expression were evaluated by using lncRNA and mRNA microarray or real‐time polymerase chain reaction technique. LncRNA‐mRNA coexpression network assay, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed. The results showed that 1275 lncRNAs were differentially expressed in septic myocardium compared with those in the control group. A total of 2769 mRNAs were dysregulated in septic mice heart, most of which are mainly related to the process of inflammation, mitochondrial metabolism, oxidative stress, and apoptosis. Coexpression network analysis showed that 14 lncRNAs were highly correlated with 11 mitochondria‐related differentially expressed mRNA. Among all lncRNAs and their cis‐acting mRNAs, 41 lncRNAs‐mRNA pairs (such as NONMMUG004378 and Apaf1 gene) were enriched in GO terms and KEGG pathways. In summary, we gained some specific lncRNAs and their potential target mRNAs that might be involved in mitochondrial dysfunction in septic myocardium. These findings provide a panoramic view of lncRNA and might allow developing new treatment strategies for sepsis.
Long noncoding RNA LEF1‐AS1 acts as a microRNA‐10a‐5p regulator to enhance MSI1 expression and promote chemoresistance in hepatocellular carcinoma cells through activating AKT signaling pathway
Authors: Gao Jun , Dai Chao , Yu Xin , Yin Xiang‐Bao , Zhou Fan 
Published: August 2020

AbstractLong noncoding RNAs (lncRNAs) contribute to the development of hepatocellular carcinoma (HCC), which could regulate various HCC biological characteristics. Here, the study seeks to investigate the role of lncRNA LEF1‐AS1 in HCC cell chemoresistance by regulating microRNA (miR)‐10a‐5p and Musashi1 (MSI1). The microarray‐based analysis was employed to identify the HCC‐related lncRNA‐miRNA‐gene regulatory network. Expression patterns of LEF1‐AS1, miR‐10a‐5p, and MSI1 in the HCC cell lines, tissues were accessed by means of reverse transcription‐quantitative polymerase chain reaction. Next, the interaction among LEF1‐AS1, miR‐10a‐5p, and MSI1 in HCC was accessed by bioinformatics and dual‐luciferase reporter gene assay. Then, the cell line resistant to cisplatin was established, which was then treated with sh/oe‐lncRNA LEF1‐AS1, miR‐10a‐5p‐mimic, and oe/sh‐MSI1 vectors alone or in combination. Afterward, the effect of LEF1‐AS1, miR‐10a‐5p, and MSI1 on HCC cell chemoresistance, proliferation, and apoptosis was assessed. At last, in vivo experiments confirmed the role of MSI1 in tumor growth and chemoresistance in HCC. LEF1‐AS1 might potentially affect the growth and chemoresistance of HCC cells by regulating miR‐10a‐5p and MSI1. LEF1‐AS1 and MSI1 expression patterns were elevated, while miR‐10a‐5p was repressed in HCC tissues and cell lines. LEF1‐AS1 combined to miR‐10a‐5p and regulated MSI1, thereby activating the protein kinase B (AKT) signaling pathway. Knockdown of LEF1‐AS1 and MSI1 or elevation of miR‐10a‐5p compromised the proliferation of Huh7 cell line resistant to DDP and promoted its chemosensitivity and apoptosis. At last, these in vitro findings were also confirmed in vivo. Our results unraveled LEF1‐AS1 acts as a miR‐10a‐5p modulator to promote chemoresistance of HCC cells by stimulating MSI1 and activating the AKT signaling pathway, which might provide a novel therapeutic target for HCC.
Cancer metastasis‐associated protein 1 localizes to the nucleolus and regulates pre‐rRNA synthesis in cancer cells
Authors: Liu Jian , Li Chunxiao , Xue Hongsheng , Li Lina , Liu Qun , Wang Haijuan , Wen Tao , Qian Haili 
Published: August 2020

AbstractMetastasis‐associated protein 1 (MTA1) is a critical component of the nucleosome remodeling and histone deacetylase (NuRD) complex. MTA1 has several biological functions, and it is closely associated with the malignant properties of human cancers; however, the mechanisms and subcellular localization of MTA1 in cells remain unclear. Some initial studies indicated that MTA1 was absent from the nucleolus; however, several NuRD components were recently found to be present in the nucleolus, where they regulate preribosomal RNA (pre‐rRNA) transcription. In this study, we demonstrated that MTA1 is definitely localized to the nucleolus and regulates pre‐rRNA transcription, which is consistent with the recent reports on NuRD. To determine if MTA1 was present in the nucleolus, we utilized the following complementary molecular approaches: immunofluorescence, GFP‐tag tracking, immunoelectron microscopy, and immunoprecipitation (IP). To examine the role of MTA1 in rRNA synthesis, we performed quantitative polymerase chain reaction analysis. We revealed that both endogenous and exogenous MTA1 showed apparent granule‐like nucleolar subcellular localization. MTA1 interacts with two major resident nucleolar proteins, nucleolin and nucleophosmin. Immunofluorescent colocalization analyses showed that MTA1 localizes to the fibrillarin‐deficient regions of the nucleolus, and Co‐IP experiments indicated that there was no interaction between MTA1 and fibrillarin; further, fibrillarin was not identified in the MTA1 interactome. Loss‐ and gain‐of‐function studies indicated that MTA1 promotes pre‐rRNA transcription in cancer cells. Collectively, our data identify MTA1 as a novel nucleolar protein, and activation of pre‐rRNA transcription in cancer cells may be an alternative mechanism by which MTA1 promotes malignancies.
A longitudinal study of the impact of school delinquency on self‐worth development among Black American adolescents
Authors: Tomek Sara , Moore Heather , Hooper Lisa M. , Bolland Anneliese C. , Robinson Cecil D. , Bolland John M. 
Published: August 2020

AbstractPrevious research found adolescents with low self‐worth often utilize delinquency as a method of “self‐enhancing” as proposed by Kaplan, which suggests the effects of delinquency can be both enhancing and damaging to adolescents' later reports of self‐worth. We tested Kaplan's self‐enhancing thesis to determine the extent to which different levels of self‐worth in early adolescents foretell long‐term levels of self‐worth associated with delinquency among adolescents placed at‐risk. Data from a sample of 982 primarily Black American (95%) adolescents living in high‐poverty neighborhoods were analyzed using global and behavioral self‐worth measures collected annually between the ages of 12–17, with school delinquency as the self‐enhancing mechanism. Gender (45% female, 55% male) and baseline self‐worth measures were included in the model. We found empirical support for the positive effects of school delinquency consistent with self‐enhancing theories, although with younger female participants only. Specifically, engaging in delinquent behaviors at age 12 had a positive effect on a females' behavioral self‐worth. There were, however, differential effects for males. Although delinquency increased self‐worth among females in the short‐term, long‐term effects were negative, as greater school delinquency resulted in lower self‐worth at age 17. Additional gender results and implications for findings are discussed.
Placement of chronically homeless into different types of permanent supportive housing before and after a coordinated entry system: The influence of severe mental illness, substance use disorder, and dual diagnosis on housing configuration and intensity of services
Authors: Dickson‐Gomez Julia , Quinn Katherine , McAuliffe Timothy , Bendixen Arturo , Ohlrich Jessica 
Published: August 2020

AbstractAimsPermanent supportive housing (PSH) is designed to house people who experience chronic homelessness with one or more of the following: serious mental illness (SMI), substance use disorders (SUD) or human immunodeficiency virus. The Department of Housing and Urban Development has required major metropolitan areas to develop a coordinated entry system (CES) to prioritize access to PSH to those who need it the most. The aim of this paper is to determine whether PSH residents with SMI, SUD, or dual diagnosis were more likely to be housed after implementation of CES and were more likely to be housed in housing models with more intensive services provided.MethodsA cross‐sectional survey with 855 residents of different PSH models.ResultsThose with SMI, SUD, or dual diagnosis were not more likely to be housed using the CES but were more likely to be housed in higher intensity service programs.ConclusionsThose with SMI are more likely to be housed in PSH with high‐intensity services.
Monotonic torsional resistance and fatigue resistance of novel SCOPE RS instruments
Authors: Orhan Ekim Onur , Ertuğrul İhsan Furkan , Tosun Samet 
Published: August 2020

AbstractTo evaluate monotonic torsional resistance and fatigue resistance of novel SCOPE RS instruments and to compare ROTATE, Hyflex CM, OneCurve, and ProTaper Next instruments as a counterpart. The instrument groups were as follows: SCOPE RS; ROTATE; HyflexCM; OneCurve; ProTaperNext. The cyclic fatigue resistance of unused instruments was tested at both room and body temperatures (n = 10). The time to fracture, the mean number of cycles until failure, and the length of the fractured fragments were analyzed. The monotonic torsional resistance of new instruments was measured in accordingly American National Standards/American Dental Association No. 28 and International Organization for Standardization 3630–1:2008 specifications (n = 10). The maximum torque and angular deflection at break were recorded. The fractographic examination was performed by scanning electron microscope. Energy‐dispersive X‐ray spectroscopy was used to investigate the microstructure of NiTi instruments. One‐way ANOVA with Games‐Howell Post Hoc multiple comparisons tests were used (p < .05). The ROTATE had superior cyclic fatigue resistance than other groups in both temperature conditions (p < .01). However, it exhibited lower torsional resistance than SCOPE RS (p < .01). SCOPE RS had superior torsional resistance than other groups (p < .01). Micrographs revealed typical features of fatigue behaviors in all groups. Weight percentages of the Ni and Ti revealed similarity for all instruments. The novel SCOPE RS instruments presented superior monotonic torsional resistance but failed to show any improvement in the cyclic fatigue resistance compared with its counterparts, ROTATE, HyflexCM, and OneCurve.
Skeletal and muscular pelvic morphology of hillstream loaches (Cypriniformes: Balitoridae)
Authors: Crawford Callie H. , Randall Zachary S. , Hart Pamela B. , Page Lawrence M. , Chakrabarty Prosanta , Suvarnaraksha Apinun , Flammang Brooke E. 
Published: August 2020

AbstractThe rheophilic hillstream loaches (Balitoridae) of South and Southeast Asia possess a range of pelvic girdle morphologies, which may be attributed to adaptations for locomotion against rapidly flowing water. Specifically, the connectivity of the pelvic plate (basipterygium) to the vertebral column via a sacral rib, and the relative size and shape of the sacral rib, fall within a spectrum of three discrete morphotypes: long, narrow rib that meets the basipterygium; thicker, slightly curved rib meeting the basipterygium; and robust crested rib interlocking with the basipterygium. Species in this third category with more robust sacral rib connections between the basipterygium and vertebral column are capable of walking out of water with a tetrapod‐like lateral‐sequence, diagonal‐couplet gait. This behavior has not been observed in species lacking direct skeletal connection between the vertebrae and the pelvis. The phylogenetic positions of the morphotypes were visualized by matching the morphological features onto a novel hypothesis of relationships for the family Balitoridae. The morphotypes determined through skeletal morphology were correlated with patterns observed in the pelvic muscle morphology of these fishes. Transitions towards increasingly robust pelvic girdle attachment were coincident with a more anterior origin on the basipterygium and more lateral insertion of the muscles on the fin rays, along with a reduction of the superficial abductors and adductors with more posterior insertions. These modifications are expected to provide a mechanical advantage for generating force against the ground. Inclusion of the enigmatic cave‐adapted balitorid Cryptotora thamicola into the most data‐rich balitorid phylogeny reveals its closest relatives, providing insight into the origin of the skeletal connection between the axial skeleton and basipterygium.
Dynamical downscaling simulation of the East Asian summer monsoon in a regional Climate‐Weather Research and Forecasting model
Authors: Li Qingquan , Wang Tao , Wang Fang , Liang Xin‐Zhong , Zhao Chongbo , Dong Lili , Zhao Chunyu , Xie Bing 
Published: August 2020

AbstractA regional Climate‐Weather Research and Forecasting (CWRF) model with a 30‐km horizontal resolution was applied to simulate the East Asian summer monsoon (EASM) and climate in China from 1980 to 2016. As compared with observations and reanalysis data, the model can reasonably reproduce the spatial distributions of the climatological mean atmospheric circulation and water vapour transport in East Asia, as well as the seasonal advance and retreat of EASM and rain bands. The correlation coefficient between the EASM circulation index in the simulation and reanalysis is .97. The model can well represent the geographic distributions of summer mean temperature and precipitation over most of China. However, model biases still exist, in particular the skill in the Yangtze–Huaihe River basin is relatively low. The simulated climatological mean temperature and precipitation deviations from observations may be related to the model's systematic circulation biases, with a thicker lower troposphere (i.e., higher temperature between 500 and 1,000 hPa) over most of China (except for the south and southwest) and a thinner one over the coastal oceans in summertime. Compared with the reanalysis, the model overestimates the land‐ocean thermal contrast, which causes the South Asian High shifted to the north and east, as well as the subtropical high and tropical convective activity located further north and persisted longer. Consequently, more water vapour transports northward, leading to more precipitation over North China–Northeast China and less precipitation over the Yangtze and Huaihe River basin. Therefore, the enhanced land‐ocean thermal contrast may be a major factor for the stronger EASM in the model, and corresponding precipitation and temperature biases in China. This analysis provides important information for further improving model performance in EASM simulation.
Appropriate Phosphorus Intake by Parenteral Nutrition Prevents Metabolic Bone Disease of Prematurity in Extremely Low‐Birth‐Weight Infants
Authors: Motokura Kouji , Tomotaki Seiichi , Hanaoka Shintaro , Yamauchi Takeru , Tomotaki Hiroko , Iwanaga Kougoro , Niwa Fusako , Takita Junko , Kawai Masahiko 
Published: August 2020

AbstractBackgroundMetabolic bone disease (MBD) is a common disorder in extremely low‐birth‐weight (ELBW) infants. However, no studies have investigated whether high‐dose calcium (Ca) and phosphorus (P) supplementation by parenteral nutrition (PN) prevents MBD in ELBW infants. This study aimed to identify the effect of PN on MBD in ELBW infants.MethodsWe retrospectively analyzed ELBW infants who were admitted between April 2011 and March 2017. ELBW infants were divided into the low‐P group (n = 22) and the high‐P group (n = 26) according to the dose of parenteral P supply. Biochemical and radiological markers of MBD and treatments were analyzed.ResultsMean daily parenteral intake of Ca and P in the first week was significantly higher in the high‐P group than in the low‐P group (both P ≤ .001). Serum alkaline phosphatase (ALP) levels were significantly higher in the low‐P group than in the high‐P group in the first month. ELBW infants in the low‐P group received alfacalcidol much more frequently than those in the high‐P group. There was a trend of a higher rate of x‐ray changes in the low‐P group than in the high‐P group. No infants developed bone fractures.ConclusionAppropriate P intake by PN is required to ensure high Ca intake, reduce ALP levels in the first month, and prevent MBD from hyperparathyroidism and does not worsen x‐ray findings in ELBW infants.
2,3,5,4′‐Tetrahydroxystilbene‐2‐O‐β‐d‐glucoside promotes the effects of dental pulp stem cells on rebuilding periodontal tissues in experimental periodontal defects
Authors: Lin Chi‐Yu , Tsai Min‐Shi , Kuo Po‐Jan , Chin Yu‐Tang , Weng I‐Tsen , Wu Yen , Huang Haw‐Ming , Hsiung Chao‐Nan , Lin Hung‐Yun , Lee Sheng‐Yang 
Published: August 2020

AbstractBackgroundThis study aimed to investigate the regenerative effects of 2,3,5,4′‐tetrahydroxystilbene‐2‐O‐β‐d‐glucoside (THSG)‐treated human dental pulp stem cells (DPSC) on the healing of experimental periodontal defects in rats.MethodsThe maxillary first molars of 30 male Sprague–Dawley rats were extracted, and after healing, bilateral periodontal defects were surgically created mesially in second molars. The defects were treated with Matrigel (as control), DPSC, or DPSC + THSG. After 2 weeks, the healed defects were evaluated using microcomputed tomography and through histological and immunohistochemical analyses.ResultsIn the microcomputed tomography analysis, more new bone formation in the DPSC and DPSC + THSG groups was observed compared with the control group. The periodontal bone supporting ratio in site with DPSC + THSG was significantly higher than that in DPSC. Histologically, an enhanced new bone formation and more significant periodontal attachment were observed in the DPSC + THSG group. The expression levels of proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), and osteopontin (OPN) in the DPSC + THSG group were significantly greater than those in other groups.ConclusionsTHSG‐revolutionized DPSCs significantly shortened the regenerative period of periodontal defects by enhancing the cell recruitment and possibly the angiogenesis in rat models, which illustrate the critical implications for a clinical application and provide a novel tactic for periodontitis treatment.
Effect of ultrasound‐assisted extraction on the structure and emulsifying properties of peanut protein isolate
Authors: Sun Xiaoyang , Zhang Wen , Zhang Lifen , Tian Shaojun , Chen Fusheng 
Published: August 2020

AbstractBACKGROUNDWith an increasing demand for edible protein, research on new extraction methods is attracting more attention. The effects of such methods on functional properties are important. The present study aimed to evaluate the effect of ultrasound‐assisted extraction on the extraction efficiency, structure, and the emulsifying properties of peanut protein isolate (PPI).RESULTSUltrasound‐assisted extraction significantly improved extraction efficiency and shortened the processing time. The nanostructure, molecular weight distribution, and particle size of PPI were altered by ultrasound‐assisted extraction. The emulsifying properties of the PPI from ultrasound‐assisted extraction were significantly improved compared with alkaline extraction. Peanut protein isolate had lower molecular weight fractions, higher levels of hydrophobic amino acids, and the highest fluorescence intensity with ultrasound intensity, temperature, and time of 3.17 W cm−3, 35 °C, and 30 min, respectively. These contributed to the higher emulsifying activity index and emulsifying stability index of the PPI emulsions. The uniform distribution of droplets and smaller particle size of the PPI emulsions was also observed.CONCLUSIONThe results suggested that ultrasound can be used to induce the conformational changes to modify the interfacial association between protein‐oil phases, thereby improving the emulsifying properties of peanut protein. © 2020 Society of Chemical Industry
Essential oil from leaves of Eugenia calycina Cambes: Natural larvicidal against Aedes aegypti
Authors: Silva Marcus VSG , Silva Sheila A , Teixera Thaise Lara , De Oliveira Alberto , Morais Sérgio AL , Da Silva Claudio Vieira , Espindola Laila S , Sousa Raquel MF 
Published: August 2020

AbstractBACKGROUNDEugenia calycina is an endemic species in the Brazilian savannah (the Cerrado) and it is threatened with extinction. Several species of Eugenia are used as insecticides or insect repellents. No data are available on the larvicidal activity of E. calycina. The chemical composition of the essential oil (EO) from leaves of Eugenia calycina was analyzed by gas chromatography coupled to mass spectrometry (GC–MS) and the larvicidal activity against Aedes aegypti larvae in the third stage of development was studied.RESULTSOxygenated and non‐oxygenated sesquiterpenes were identified, and the main compounds were bicyclogermacrene, spathulenol, and β‐caryophyllene. The EO was fractionated in a chromatographic column and three compounds were isolated and identified: spathulenol, aromadendrane‐4β,10α‐diol, and 1β‐11‐dihydroxy‐5‐eudesmene. It is the first time that the last two compounds have been identified in E. calycina. The exposure times in the larvicidal test were 24 h and 48 h and the LC50 values obtained were 199.3 and 166.4 μg mL−1. The cytotoxicity of the EO in mammalian cells (HeLa and Vero) was evaluated for 24 and 48 h of incubation. The cytotoxic concentrations of the EO for HeLa and Vero cells (266.8 ± 46.5 and 312.1 ± 42.5 μg mL−1, respectively) in 48 h of exposure were higher than the LC50, showing low cytotoxicity at the concentration exhibiting larvicidal activity, resulting in a positive selectivity index.CONCLUSIONThese results indicate that the EO of E. calycina showed high activity against the A. aegypti larvae but lower cytotoxicity to mammalian cells. The leaves of E. calycina are therefore a very promising source of natural larvicidal products. © 2020 Society of Chemical Industry
The publication of health sciences theses in Turkey: A study of Ege University
Authors: Karaca Ozan , Çalışkan S. Ayhan , Durak Halil İbrahim 
Published: August 2020

AbstractThis study aimed to determine the academic publication rate of health sciences graduate theses, as well as the factors that influence researchers to publish. The study took place in Ege University Institute of Health Sciences, Turkey, and used a correlated research model to analyse both qualitative and quantitative data. We obtained data from 159 graduate students selected from 437 who graduated between 1 September 2009 and 31 August 2014. From this group, 76 of the theses were published as 141 items. Of these, 93 (66%) were journal articles, of which 51 (36.2%) were published in journals indexed by Science Citation Index (SCI)/SCI‐Expanded/Emerging Sources Citation Index (ESCI). In the multiple regression analysis, we found that employment in a university increased the probability of producing publications from theses by 8.54 times (odds ratio [OR], 95% confidence interval [CI] = 3.954–18.440) and that encouragement from a supervisor increased the same by 2.79 times (OR, 95% CI = 1.021–7.640). Reasons for not publishing their theses were classified into 11 items, of which thematic analysis showed that the most prominent reasons were lack of time (n = 18, 24.3%) and workload (n = 15, 20.2%). This suggests that interventions targeting these two factors may result in increased publication of theses.Key points
Half of graduates' theses were converted into published items with no significant differences according to gender and graduate programme.
Successful publication of items derived from theses is positively influenced by employment in university and receiving encouragement from a supervisors.
The main reason for not publishing theses was workload and lack of time.