bluuebunny/crossref_metadata_2025_split huggingface dataset:
A transparent and defensible process for applicant selection within a Canadian emergency medicine residency program
Authors: Paterson Quinten S. , Hartmann Riley , Woods Rob , Martin Lynsey J. , Thoma Brent 
Published: January 2020

ABSTRACTObjectivesThe Canadian Resident Matching Service (CaRMS) selection process has come under scrutiny due to the increasing number of unmatched medical graduates. In response, we outline our residency program's selection process including how we have incorporated best practices and novel techniques.MethodsWe selected file reviewers and interviewers to mitigate gender bias and increase diversity. Four residents and two attending physicians rated each file using a standardized, cloud-based file review template to allow simultaneous rating. We interviewed applicants using four standardized stations with two or three interviewers per station. We used heat maps to review rating discrepancies and eliminated rating variance using Z-scores. The number of person-hours that we required to conduct our selection process was quantified and the process outcomes were described statistically and graphically.ResultsWe received between 75 and 90 CaRMS applications during each application cycle between 2017 and 2019. Our overall process required 320 person-hours annually, excluding attendance at the social events and administrative assistant duties. Our preliminary interview and rank lists were developed using weighted Z-scores and modified through an organized discussion informed by heat mapped data. The difference between the Z-scores of applicants surrounding the interview invitation threshold was 0.18-0.3 standard deviations. Interview performance significantly impacted the final rank list.ConclusionsWe describe a rigorous resident selection process for our emergency medicine training program which incorporated simultaneous cloud-based rating, Z-scores, and heat maps. This standardized approach could inform other programs looking to adopt a rigorous selection process while providing applicants guidance and reassurance of a fair assessment.
Socio-economic-related health inequality in non-communicable diseases among older people in Viet Nam
Authors: Le Duc Dung , Leon-Gonzalez Roberto , Giang Thanh Long , Nguyen Anh Tuyet 
Published: January 2020

AbstractThis study contributes to a growing literature body of studies aimed at explaining socio-economic-related health inequality in non-communicable diseases (NCDs), with a focus on older people who are commonly affected by socio-economic gradient in later life. It identifies factors associated with self-reported NCDs and examines socio-economic-related health inequality in self-reported NCDs between rural and urban Vietnamese older people. This cross-sectional study utilised data from the Viet Nam Ageing Survey. A sample of 2,682 older people aged 60 and over (urban = 703, rural = 1,979) was analysed. Concentration indices were computed to measure socio-economic inequalities in self-reported NCDs. Concentration index decomposition analysis was performed to determine the relative contributions of the determinants to explaining those inequalities. Significant socio-economic inequalities in self-reported NCDs favouring the rich were found, in which the degree of inequality was more pronounced in urban areas than in their rural counterparts. Household wealth and social health insurance were the main drivers contributing to increased socio-economic inequalities in self-reported NCDs in urban and rural areas, respectively. Among disadvantaged groups, older people living alone, with lowest wealth and with social health insurance had highest probability of reporting at least one NCD for both areas. Public policies aimed at narrowing wealth gaps and expanding and improving principle roles of social health insurance should prioritise the most disadvantaged groups in order to achieve health equality.
Gas Flow by Invasion Percolation Through the Hydrate Stability Zone
Authors: Meyer Dylan W. , Flemings Peter B. , You Kehua , DiCarlo David A. 
Published: January 2020

AbstractWe describe gas flow through the hydrate stability zone as a growing gas network with hydrate forming on its edges. The diffusion‐limited model successfully describes the dynamics of hydrate formation observed experimentally. We obtain an expression for the rate of hydrate growth in a region where gas and water coexist. Our modeled rate of hydrate formation is much lower than predicted with a kinetics‐based approach, and it more successfully simulates the hydrate formation rate observed in geologic systems. Current simulation approaches can readily incorporate our approach to describe hydrate formation in geological systems.
Advective Fluxes in the Martian Regolith as a Mechanism Driving Methane and Other Trace Gas Emissions to the Atmosphere
Authors: Viúdez‐Moreiras D. , Arvidson R. E. , Gómez‐Elvira J. , Webster C. , Newman C. E. , Mahaffy P. , Vasavada A. R. 
Published: January 2020

AbstractAdvective fluxes influence methane and CO2 soil emissions into the atmosphere on Earth and may drive trace gas emissions in the Mars atmosphere. However, their relevance in the Martian regolith has not been evaluated to date. Our regolith transport simulations show that advective fluxes can be relevant under Martian conditions and may drive the methane abundance detected by Mars Science Laboratory. Trace gas emissions would be highest in regions where winds interact with topography. Emissions in these regions may be further enhanced by time‐varying pressure fields produced by diurnal thermal tides and atmospheric turbulence. Trace gases such as methane should be emitted or produced from the first layers of regolith, or quickly transported to this region from a deeper reservoir through fractured media.
A Method for Estimating the Crustal Azimuthal Anisotropy and Moho Orientation Simultaneously Using Receiver Functions
Authors: Wang Pan , Huang Zhouchuan , Wang Xingchen 
Published: January 2020

AbstractThe P‐to‐S converted phase at the Moho (the Pms phase) contains important information regarding the crustal structure. Here, we employ a grid search approach to simultaneously extract the bulk crustal azimuthal anisotropy and Moho orientation using receiver functions. The solution of this approach is the weighted average of two individual subfunctions that separately analyze the birefringence and arrival time of the Pms phase. Since a tilted Moho deflects the polarized components of the Pms phase, the Pms birefringence subfunction minimizes the energy on all the transverse polarization components (i.e., the transverse polarization direction before Pms splitting). The implementation of this subfunction is similar to that of the transverse component minimization method, but the Moho orientation is considered. The Pms arrival time subfunction takes into account both the crustal anisotropy and the Moho orientation to predict and fit Pms arrivals. According to extensive synthetic experiments, this approach is remarkably robust to the presence of strong anisotropy, a steep Moho, and slightly tilted fast axes (less than 30°). This approach is also highly tolerant of random noise and data with uneven azimuthal coverage. We apply this approach to three permanent stations on the Tibetan Plateau. The resulting measurements boast a high quality and are consistent with the crustal anisotropy and Moho orientation revealed in previous studies.
Spatiotemporal Properties of Sub‐Rayleigh and Supershear Ruptures Inferred From Full‐Field Dynamic Imaging of Laboratory Experiments
Authors: Rubino V. , Rosakis A. J. , Lapusta N. 
Published: January 2020

AbstractMany earthquakes propagate at sub‐Rayleigh speeds. Earthquakes propagating at supershear speeds, though less common, are by far more destructive. Hence, it is important to quantify the motion characteristics associated with both types of earthquake ruptures. Here we report on the spatiotemporal properties of dynamic ruptures measured in our laboratory experiments using the dynamic digital image correlation technique. Earthquakes are mimicked by the frictional rupture propagating along the interface of two Homalite plates. Digital images of the propagating ruptures are captured by an ultrahigh‐speed camera and processed with digital image correlation in order to produce sequences of evolving displacement and velocity maps. Our measurements reveal the full‐field structure of the velocity components, bridge the gap between previous spatially sparse velocimeter measurements available only at two to three locations, and enable us to quantify the attenuation patterns away from the interface.
Shifts in the Distribution and Fate of Sedimentary Polycyclic Aromatic Hydrocarbons Within the Estuarine‐Inner Shelf Areas of the East China Sea: Response to Human‐Induced Catchment Change
Authors: Wang Chenglong , Hao Zhe , Feng Ziyue , Zhang Chuchu , Li Yali , Yu Wenwen , Zou Xinqing 
Published: January 2020

Key Points

Distribution patterns of PAHs changed drastically due to human‐induced catchment changes
Depositional center of PAHs in the ECS transferred southward
River‐dominated margins will face this threat worldwide
Specific, reversible G1 arrest by UCN-01 in vivo provides cytostatic protection of normal cells against cytotoxic chemotherapy in breast cancer
Authors: Mull Benjamin B. , Livingston J. Andrew , Patel Nalini , Bui Tuyen , Hunt Kelly K. , Keyomarsi Khandan 
Published: January 2020

AbstractBackgroundLow-dose UCN-01 mediates G1 arrest in normal proliferating cell lines with an intact G1 to S transition but not tumour cells with a deregulated G1 to S checkpoint. Here we hypothesised that UCN-01 is effective in mediating a selective, reversible G1 arrest of normal proliferating cells, resulting in decreased chemotoxicity, improved tolerance and enhanced chemotherapeutic efficacy in vivo in both non-tumour-bearing mice and in breast cancer cell line xenograft models.MethodsMurine small bowel epithelium was used to examine the kinetics and mechanism of low-dose UCN-01-mediated arrest of normal proliferating cells and if it can protect tumour-bearing mice (MDA-MB-468 xenografts) against the toxic effects of chemotherapy (5-fluorouricil (5-FU)) allowing for its full therapeutic activity.ResultsUCN-01 causes significant, reversible arrest of normal gut epithelial cells at 24 h; this arrest persists for up to 7 days. Normal cellular proliferation returns by 2 weeks. Pre-treatment of both non-tumour-bearing and MDA-MB-468 tumour-bearing mice with UCN-01 prior to bolus 5-FU (450 mg/kg) yielded enhanced therapeutic efficacy with significantly decreased tumour volumes and increased survival.ConclusionsUCN-01 mediates a specific, reversible G1 arrest of normal cells in vivo and provides a cytoprotective strategy that decreases toxicity of cytotoxic chemotherapy without compromising efficacy.
ADT-OH, a hydrogen sulfide-releasing donor, induces apoptosis and inhibits the development of melanoma in vivo by upregulating FADD
Authors: Cai Fangfang , Xu Huangru , Cao Nini , Zhang Xiangyu , Liu Jia , Lu Yanyan , Chen Jia , Yang Yunwen , Cheng Jian , Hua Zi-Chun , Zhuang Hongqin 
Published: January 2020

AbstractHydrogen sulfide (H2S) is now widely considered the third endogenous gasotransmitter and plays critical roles in cancer biological processes. In this study, we demonstrate that 5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione (ADT-OH), the most widely used moiety for synthesising slow-releasing H2S donors, induces melanoma cell death in vitro and in vivo. Consistent with previous reports, ADT-OH inhibited IκBɑ degradation, resulting in reduced NF-κB activation and subsequent downregulation of the NF-κB-targeted anti-apoptotic proteins XIAP and Bcl-2. More importantly, we found that ADT-OH suppressed the ubiquitin-induced degradation of FADD by downregulating the expression of MKRN1, an E3 ubiquitin ligase of FADD. In addition, ADT-OH had no significant therapeutic effect on FADD-knockout B16F0 cells or FADD-knockdown A375 cells. Based on these findings, we evaluated the combined effects of ADT-OH treatment and FADD overexpression on melanoma cell death in vivo using a mouse xenograft model. As expected, tumour-specific delivery of FADD through a recombinant Salmonella strain, VNP-FADD, combined with low-dose ADT-OH treatment significantly inhibited tumour growth and induced cancer cell apoptosis. Taken together, our data suggest that ADT-OH is a promising cancer therapeutic drug that warrants further investigation into its potential clinical applications.
Maximizing the ovarian reserve in mice by evading LINE-1 genotoxicity
Authors: Tharp Marla E. , Malki Safia , Bortvin Alex 
Published: January 2020

AbstractFemale reproductive success critically depends on the size and quality of a finite ovarian reserve. Paradoxically, mammals eliminate up to 80% of the initial oocyte pool through the enigmatic process of fetal oocyte attrition (FOA). Here, we interrogate the striking correlation of FOA with retrotransposon LINE-1 (L1) expression in mice to understand how L1 activity influences FOA and its biological relevance. We report that L1 activity triggers FOA through DNA damage-driven apoptosis and the complement system of immunity. We demonstrate this by combined inhibition of L1 reverse transcriptase activity and the Chk2-dependent DNA damage checkpoint to prevent FOA. Remarkably, reverse transcriptase inhibitor AZT-treated Chk2 mutant oocytes that evade FOA initially accumulate, but subsequently resolve, L1-instigated genotoxic threats independent of piRNAs and differentiate, resulting in an increased functional ovarian reserve. We conclude that FOA serves as quality control for oocyte genome integrity, and is not obligatory for oogenesis nor fertility.
Melanoblast transcriptome analysis reveals pathways promoting melanoma metastasis
Authors: Marie Kerrie L. , Sassano Antonella , Yang Howard H. , Michalowski Aleksandra M. , Michael Helen T. , Guo Theresa , Tsai Yien Che , Weissman Allan M. , Lee Maxwell P. , Jenkins Lisa M. , Zaidi M. Raza , Pérez-Guijarro Eva , Day Chi-Ping , Ylaya Kris , Hewitt Stephen M. , Patel Nimit L. , Arnheiter Heinz , Davis Sean , Meltzer Paul S. , Merlino Glenn , Mishra Pravin J. 
Published: January 2020

AbstractCutaneous malignant melanoma is an aggressive cancer of melanocytes with a strong propensity to metastasize. We posit that melanoma cells acquire metastatic capability by adopting an embryonic-like phenotype, and that a lineage approach would uncover metastatic melanoma biology. Using a genetically engineered mouse model to generate a rich melanoblast transcriptome dataset, we identify melanoblast-specific genes whose expression contribute to metastatic competence and derive a 43-gene signature that predicts patient survival. We identify a melanoblast gene, KDELR3, whose loss impairs experimental metastasis. In contrast, KDELR1 deficiency enhances metastasis, providing the first example of different disease etiologies within the KDELR-family of retrograde transporters. We show that KDELR3 regulates the metastasis suppressor, KAI1, and report an interaction with the E3 ubiquitin-protein ligase gp78, a regulator of KAI1 degradation. Our work demonstrates that the melanoblast transcriptome can be mined to uncover targetable pathways for melanoma therapy.
Electrophysiological dynamics of antagonistic brain networks reflect attentional fluctuations
Authors: Kucyi Aaron , Daitch Amy , Raccah Omri , Zhao Baotian , Zhang Chao , Esterman Michael , Zeineh Michael , Halpern Casey H. , Zhang Kai , Zhang Jianguo , Parvizi Josef 
Published: January 2020

AbstractNeuroimaging evidence suggests that the default mode network (DMN) exhibits antagonistic activity with dorsal attention (DAN) and salience (SN) networks. Here we use human intracranial electroencephalography to investigate the behavioral relevance of fine-grained dynamics within and between these networks. The three networks show dissociable profiles of task-evoked electrophysiological activity, best captured in the high-frequency broadband (HFB; 70–170 Hz) range. On the order of hundreds of milliseconds, HFB responses peak fastest in the DAN, at intermediate speed in the SN, and slowest in the DMN. Lapses of attention (behavioral errors) are marked by distinguishable patterns of both pre- and post-stimulus HFB activity within each network. Moreover, the magnitude of temporally lagged, negative HFB coupling between the DAN and DMN (but not SN and DMN) is associated with greater sustained attention performance and is reduced during wakeful rest. These findings underscore the behavioral relevance of temporally delayed coordination between antagonistic brain networks.
Temporal inhibition of autophagy reveals segmental reversal of ageing with increased cancer risk
Authors: Cassidy Liam D. , Young Andrew R. J. , Young Christopher N. J. , Soilleux Elizabeth J. , Fielder Edward , Weigand Bettina M. , Lagnado Anthony , Brais Rebecca , Ktistakis Nicholas T. , Wiggins Kimberley A. , Pyrillou Katerina , Clarke Murray C. H. , Jurk Diana , Passos Joao F. , Narita Masashi 
Published: January 2020

AbstractAutophagy is an important cellular degradation pathway with a central role in metabolism as well as basic quality control, two processes inextricably linked to ageing. A decrease in autophagy is associated with increasing age, yet it is unknown if this is causal in the ageing process, and whether autophagy restoration can counteract these ageing effects. Here we demonstrate that systemic autophagy inhibition induces the premature acquisition of age-associated phenotypes and pathologies in mammals. Remarkably, autophagy restoration provides a near complete recovery of morbidity and a significant extension of lifespan; however, at the molecular level this rescue appears incomplete. Importantly autophagy-restored mice still succumb earlier due to an increase in spontaneous tumour formation. Thus, our data suggest that chronic autophagy inhibition confers an irreversible increase in cancer risk and uncovers a biphasic role of autophagy in cancer development being both tumour suppressive and oncogenic, sequentially.
Microendoscopy detects altered muscular contractile dynamics in a mouse model of amyotrophic lateral sclerosis
Authors: Chen Xuefeng , Sanchez Gabriel N. , Schnitzer Mark J. , Delp Scott L. 
Published: January 2020

AbstractAmyotrophic lateral sclerosis (ALS) is a fatal disease involving motor neuron degeneration. Effective diagnosis of ALS and quantitative monitoring of its progression are crucial to the success of clinical trials. Second harmonic generation (SHG) microendoscopy is an emerging technology for imaging single motor unit contractions. To assess the potential value of microendoscopy for diagnosing and tracking ALS, we monitored motor unit dynamics in a B6.SOD1G93A mouse model of ALS for several weeks. Prior to overt symptoms, muscle twitch rise and relaxation time constants both increased, consistent with a loss of fast-fatigable motor units. These effects became more pronounced with disease progression, consistent with the death of fast fatigue-resistant motor units and superior survival of slow motor units. From these measurements we constructed a physiological metric that reflects the changing distributions of measured motor unit time constants and effectively diagnoses mice before symptomatic onset and tracks disease state. These results indicate that SHG microendoscopy provides a means for developing a quantitative, physiologic characterization of ALS progression.
Radio-Frequency Electromagnetic Field Exposure of Western Honey Bees
Authors: Thielens Arno , Greco Mark K. , Verloock Leen , Martens Luc , Joseph Wout 
Published: January 2020

AbstractRadio-frequency electromagnetic fields (RF-EMFs) can be absorbed in all living organisms, including Western Honey Bees (Apis Mellifera). This is an ecologically and economically important global insect species that is continuously exposed to environmental RF-EMFs. This exposure is studied numerically and experimentally in this manuscript. To this aim, numerical simulations using honey bee models, obtained using micro-CT scanning, were implemented to determine RF absorbed power as a function of frequency in the 0.6 to 120 GHz range. Five different models of honey bees were obtained and simulated: two workers, a drone, a larva, and a queen. The simulations were combined with in-situ measurements of environmental RF-EMF exposure near beehives in Belgium in order to estimate realistic exposure and absorbed power values for honey bees. Our analysis shows that a relatively small shift of 10% of environmental incident power density from frequencies below 3 GHz to higher frequencies will lead to a relative increase in absorbed power of a factor higher than 3.
Clinical characteristics and treatment outcome of oropharyngeal squamous cell carcinoma in an endemic betel quid region
Authors: Chen Tseng-Cheng , Wu Chen-Tu , Ko Jenq-Yuh , Yang Tsung-Lin , Lou Pei-Jen , Wang Cheng-Ping , Chang Yih-Leong 
Published: January 2020

AbstractThe clinical characteristics of oropharyngeal squamous cell carcinoma (OPSCC) may be different between endemic and non-endemic regions of betel nut chewing. The impact of combined alcohol drinking/betel quid chewing/cigarette smoking (ABC) exposure on the survival of OPSCC remains unclear. We reviewed the medical records of OPSCC patients between 1999 and 2013. Immunohistochemical staining of p16 and HPV genotype detection by DNA Polymerase chain reaction were both performed for each tumor. A total of 300 eligible patients including 74 HPV+ OPSCC patients and 226 HPV− OPSCC patients were enrolled. The 5-year disease-free survival rates for the HPV−, HPV+ OPSCC with and without ABC patients were 49.8%, 58.4% and 94%, respectively. The 5-year overall survival rates for the patients with HPV−, HPV+ OPSCC with and without ABC patients were 46%, 57.4% and 86%, respectively. Advanced locoregionally disease (T3/T4, N2/N3), HPV- OPSCC, combined 2 or all ABC exposure were the independent adverse prognostic factors for disease-free and overall survival. Therefore, our data suggest that in an endemic region of betel quid chewing, HPV− OPSCC comprises the majority of OPSCC and has a worse survival. Combined 2 or all ABC exposure had a significant negative impact on disease-free and overall survival.
Distinct capabilities of different Gammaproteobacterial strains on utilizing small peptides in seawater
Authors: Liu Shuting , Liu Zhanfei 
Published: January 2020

AbstractProteins and peptides account for 20–75% of marine biota biomass, of which a major fraction is metabolized by bacteria, thus deciphering interactions between bacteria and peptides is important in understanding marine carbon and nitrogen cycling. To better understand capabilities of different bacterial strains on peptide decomposition, four Gammaproteobacteria (Pseudoalteromonas atlantica, Alteromonas sp., Marinobacterium jannaschii, Amphritea japonica) were incubated in autoclaved seawater amended with tetrapeptide alanine-valine-phenylalanine-alanine (AVFA), a fragment of RuBisCO. While AVFA was decomposed greatly by Pseudoalteromonas atlantica and Alteromonas sp, it remained nearly intact in the Marinobacterium jannaschii and Amphritea japonica incubations. Pseudoalteromonas and Alteromonas decomposed AVFA mainly through extracellular hydrolysis pathway, releasing 71–85% of the AVFA as hydrolysis products to the surrounding seawater. Overall, this study showed that Gammaproteobacterial strains differ greatly in their capabilities of metabolizing peptides physiologically, providing insights into interactions of bacteria and labile organic matter in marine environments.