bluuebunny/crossref_metadata_2025_split huggingface dataset:
Towards optimized breast cancer 3D spheroid mono- and co-culture models for pharmacological research and screening
Authors: Keller Florian , Rudolf Rüdiger , Hafner Mathias 
Published: December 2019

BACKGROUND: Reproducibility and physiological relevance are essential features for test systems used in preclinical cancer drug development. In this context, 3D cell culture models like spheroids or organoids have recently become attractive due to their potential of mimicking native biology. Further, to increase screening throughput and effectiveness, full automation of sample preparation and data analysis is important. OBJECTIVE: This work addressed methodological factors of spheroid cell culturing that are of particular relevance for reproducibility and physiological significance in studies investigating metabolic effects of drug treatment, including media composition, extracellular matrix, addition of stromal cells, and the quality of contrast-based readouts. METHODS: Using standardized and enriched media as well as additional basal membrane extract, spheroids were made from MCF10A human breast epithelial cells and MDA-MB-231 human breast cancer cells. In addition, co-culture spheroids of MDA-MB-231 with CCD-1137Sk human fibroblast cells were prepared. Samples were compared in terms of metabolic behaviour. Spheroid analysis employed SpheroidSizer software, confocal microscopy, and Western blotting. RESULTS: Media composition, supportive additives and the co-culture situation can massively alter the growth and metabolic behaviour of spheroids. In particular, spheroid integrity was affected by the presence of extracellular matrix components and upon long-term culturing. Due to drug- or culture-induced spheroid disintegration, automated spheroid size analysis data needed careful evaluation. CONCLUSION: Media composition, extracellular matrix, addition of stromal cells, and automated readouts are important parameters for standardized spheroid-based drug screening approaches. Contrast-based spheroid size analyses in automated high-throughput screenings need special attention, since spheroid-disaggregation or proliferation of surrounding cells may sophisticate the readouts.
An improved open-source software platform for high-throughput cultivation of phototrophic microorganisms and its application for salt tolerance experiments
Authors: Keil Karolin , Dierenbach Jonas , Tillich Ulrich M. , Steuer Ralf , Frohme Marcus 
Published: December 2019

High-throughput screening and cultivation methods offer significant potential to accelerate the development of phototrophic microorganisms into microbial cell factories. To date, however, only few suitable platforms have been reported, often lacking adequate methods for automated data handling and analysis. Here we report an improved high-throughput cultivation and screening platform that incorporates a newly developed open-source laboratory information and management system implemented using the R package Shiny. The PhotoScreener Viewer allows the user to automatically backup the collected data, provides methods for interactive graphical representation of data, including a set of statistical tests. Furthermore, the platform enables authorized users to create, view and edit their experiments online. As an advanced application study for the improved platform, we consider the response of an ethanol-producing cyanobacteria to varying salinity of the culture medium. Based on the conjecture that high-salt conditions impact the productivity of a ethanol-producing modified strain of Synechocystis sp. PCC 6803, we monitor growth, vitality and ethanol production using cultivation in 96-deep-well plates within the robotic platform. We could not observe an increased ethanol production, but rather the opposite.
KONTRIBUSI DAN EFEKTIVITAS PENERIMAAN PAJAK PARKIR TERHADAP PENDAPATAN ASLI DAERAH KOTA GORONTALO
Authors: Makausi Alma Meita , Sabijono Harijanto , Pinatik Sherly 
Published: December 2019

Parking Tax is a regional tax which can also affect the increase of local revenue. Effectiveness and contribution of Parking Tax is one tool to measure the extent of Local Government achievement in collecting Parking Tax contributes to the region’s original revenue is very important to develop a region. This study aims to (1) find out the percentage level of effectiveness of Parking Tax in Gorontalo City (2) find out how much the percentage of Parking tax revenue contribution to the Original Revenue of Gorontalo City. This study applies a descriptive qualitative analysis method. The result of research obtained by the Gorontalo City parking tax in 2014-2018 experienced a fluctuation with an average percentage of 84,95% entered the criteria quite effectively, and the parking tax in Gorontalo City contributed less to the orginal regional income because it had an average percentage of 0,06% of revenue from the Gorontalo City original income.
PENATALAKSANAAN KASUS KANDIDIASIS PSEUDOMEMBRAN AKUT PADA DIABETES MILETUS
Authors: Puspa Dewi Siti Rusdiana 
Published: December 2019

Latar Belakang: Kandidasis oral umum terjadi pada penderita diabetes miletus dikarenakan penurunan sekresi saliva di rongga mulut. Tujuan dari laporan kasus ini adalah untuk membahas manifestasi oral dan penatalaksanaan kandidasis pada penderita diabetes. Laporan kasus: Pasien perempuan berusia 56 tahun datang ke Rumah Sakit Moehammad Hoesin dengan keluhan adanya lapisan putih yang tebal pada lidah sejak 2 bulan yang lalu. Lidah terkadang terasa pedih dan pasien tidak pernah membersihkan lidahnya. Pasien memiliki riwayat penyakit diabetes miletus tidak terkontrol yang terdeteksi sejak 6 bulan yang lalu. Pemeriksaan penunjang meliputi pemeriksaan mikrobiologi dan pemeriksaan gula darah. Hasil pemeriksaan mikrobiologi dengan KOH menunjukkan yeast cell (+) dan didiagnosa Pseudomembran Kandidiasis Akut. Hasil pemeriksaan gula darah menunjukkan kadar gula darah sewaktu 306mg/dl, dan HbA1c 7,5%. Pasien diberikan medikasi berupa Nystatin drop 12 ml dengan instruksi pemakaian 2 ml 3x/hari diteteskan ke seluruh bagian yang dorsum lidah. Betadin kumur 1% 100 ml juga diberikan dengan instruksi pemakaian 3x/hari 10 ml dengan cara berkumur sehingga cairan berkontak dengan seluruh bagian yang terinfeksi kandida. Pasien juga diberikan instruksi untuk menjaga oral hygiene, menggunakan obat teratur, dan kontrol teratur terhadap kondisi sistemiknya. Kesimpulan : Penatalaksanaan oral kandidiasis sangat penting diikuti dengan mengontrol kondisi penyakit yang diderita oleh pasien, sehingga diperoleh prognosis perawatan oral yang baik pula.
ANALISIS PERBANDINGAN TINGKAT KEHILANGAN GIGI PADA PASIEN LANSIA YANG DATANG KE DOKTER GIGI DAN KE TUKANG GIGI
Authors: Asim Fauziah M. 
Published: December 2019

Lansia sangat erat hubungannya dengan penurunan semua fungsi organ tubuh termasuk fungsi dalam rongga mulut, salah satunya adalah kehilangan gigi yang banyak dialami oleh lansia seiring dengan bertambahnya usia. Penelitian ini bertujuan untuk menganlisis perbandingan tingkat kehilangan gigi pada pasien lansia dokter gigi dan tukang gigi. Metode penelitian adalah penelitian descriptive analitik dengan menggunakan pendekatan cross sectional, dengan teknik total sampling. Kriteria inklusi adalah lansia yang datang ke klinik gigi dan tukang gigi yang berada pada lokasi penelitian dan bersedia menjadi sampel penelitian. Kriteria ekslusi lansia yang tidak bersedia menjadi subyek penelitian, tidak bersedia diperiksa intra oral, dan lansia yang menderita gangguan komunikasi. Diperoleh sampel sebesar 69 responden, selanjutnya dilakukan Analisa data menggunakan T-test. Hasil penelitian didapatkan nilai P = 0,000 (P< 0,05) sehingga dapat disimpulkan terdapat perbedaan yang signifikan kehilangan gigi pada pasien dokter gigi dibandingkan dengan tukang gigi.
Upaya Mempertahankan Bisnis Radio Siaran Di Era Kemajuan Teknologi Informasi (Studi Pada Empat Perusahaan Radio Siaran di Kabupaten Kebumen)
Authors: Prasetyo Hendrawan 
Published: December 2019

The problem in this study is about how the radio broadcast business in the era of technological advancements and information, whether radio broadcasts can still maintain their existence and are still in the public interest as an effective means of entertainment and information and what radio broadcast companies do in facing business challenges in the era technological progress and information so that it can survive. The method used in this study is a qualitative research method, taking data sources using a "purposive sampling" technique with a total of 4 (four) informants including the management of radio broadcast companies in 4 (four) radio broadcast companies in the researched Kebumen Regency. Data collection in this study was carried out through observation, documentation and in-depth interviews. Observations and interviews are used to capture primary data relating to how important radio broadcast companies view technological and information progress as challenges that must be faced to maintain the existence of broadcast radio. While the study of documentation is used to capture secondary data that can be raised from various documentation about media data, broadcast radio company profiles and so on. The data analysis technique in this study is an interactive model analysis, including four components, namely data collection, data reduction, data presentation and conclusions or verification. The theory used is the theory of general communication and business communication. The locus of research in four radio broadcast companies in Kebumen District, namely: Bimasakti FM Kebumen, Mas FM Kebumen, RPFM Gombong, and Radio Gong FM Gombong, the results of this study found that advances in technology and information will raise efforts to improve the quality of broadcast radio companies in Kebumen through various ways including the ability to adjust to follow the advances in technology and information carried out by changing the conventional ways of broadcasting in a more modern way with technology-based and internet-based information. The four radio broadcast companies in Kebumen Regency were examined, although in general they had adjusted to the advancement of technology and information in order to maintain their existence. In fact there were still a number of radio broadcast companies that had not implemented streaming broadcasts so that the range that could be received by audiences or listeners was limited and not yet global. Efforts to adjust to the advancement of technology and information must, of course, be accompanied by efforts to improve the quality of human resources broadcast radio companies both internally and externally.
Keywords: business communication, existence, radio broadcast, streaming, technological progress.
Política exterior de seguridad común de la UE
Authors: Campos Orozco Francisco Javier 
Published: December 2019

La Política Exterior de Seguridad Común de la Unión Europea (PESC) opera a través de distintas herramientas y programas, los cuales llevan implícitos los elementos filosóficos que dan vida a la UE: El establecimiento de la paz entre Estados miembros, la seguridad internacional, la cohesión social, el respeto a los derechos humanos y la consolidación de la democracia, por mencionar algunos.Por ello, las misiones civiles o militares que apruebe el Consejo de Europa, a través de lo pactado en la PESC, deberán ejecutarse de manera integral, con miras a la estabilidad sostenible de la paz.En ese sentido, el presente trabajo pretende analizar las razones de la implementación de las Misiones EUTM Somalia, eucap Somalia y Operación navfor Atalanta, en el Cuerno de África, para demostrar que la imposibilidad del Estado somalí para enfrentar el crecimiento del terrorismo y la piratería ma- rítima de la zona ha sido motivo de preocupación para la UE.
O QUE DIZEM OS CONCLUDENTES EM CIÊNCIAS BIOLÓGICAS SOBRE OS PROCESSOS DE ENSINO E APRENDIZAGEM?
Authors: SILVA FRANCISCO SIDOMAR OLIVEIRA DA , COSTA JOSENILSON DA SILVA , NICOLLI ALINE ANDREIA 
Published: December 2019

O presente trabalho é resultado de uma investigação realizada com os estudantes do curso de licenciatura em ciências biológicas, da Universidade Federal do Acre, Rio Branco, com o objetivo de investigar suas visões acerca dos processos de ensino e de aprendizagem. Para garantir o desenvolvimento desta pesquisa definimos a seguinte questão de estudo: a) Na sua opinião quando e como o professor ensina e o aluno aprende? No que tange às escolhas metodológicas, a presente pesquisa constituiu-se como uma investigação com abordagem qualitativa, sendo que a coleta de dados deu-se por meio da aplicação um questionário semiestruturado. Os questionários foram respondidos por 47 discentes, distribuídos entre o sexto e oitavo períodos do curso. Para organização dos dados coletados, e auxílio nas posteriores análises, utilizamos o software Iramuteq - (Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires). As análises dos dados nos permitiram refletir sobre o fato que os futuros professores de ciências biológicas, da educação básica, têm uma visão, em certos aspectos, contraditória acerca da questão em estudo posta. Estas contradições, a nosso ver, podem influenciar o planejamento das suas aulas, delineando processos de ensino e aprendizagem atrelados à assimilação de conteúdo, que nos remete às teorias de aprendizagem pautadas na assimilação, memorização e repetição, que divergem do que se espera dos processos de ensino e de aprendizagem, em contexto atual.
The Effects of Sales Reports Business Intelligence on Employee Performance
Authors: Aini Qurotul , Harahap Eka Purnama , Faradilla Fitri 
Published: December 2019

Abstract
Selling and buying activities have been done often since long ago, and this study will be discussed about the use of technological advances, especially in the field of financial management by utilizing e-commerce. When carrying out financial data collection, staff can make recapitulation by writing each transaction in the ledger, then counting the data to be the income information. However, the process is not in tune with current technological progress, because it still use a data collection process that requires a lot of time and energy. Thus, to overcome this problem, e-commerce websites are used so that it can manage finances accurately and quickly. In this study, there are 4 (four) problems that will be overcome with 2 (two) methods, and 1 (one) solution as the answer. The advantages of e-commerce website is that it has an informative viewboard regarding financial management, which can be accessed anytime and anywhere, so it is concluded that the use of e-commerce website is able to overcome the problems found in the financial management field of Raharja Internet Cafe at Perguruan Tinggi Raharja.
FAKTOR - FAKTOR YANG MEMPENGARUHI RESPON PETANI TERHADAP PENGGUNAAN MESIN PERONTOK (POWER THRESHER) PADI (Oryza sativa, L) DI KECAMATAN PEUNARON KABUPATEN ACEH TIMUR
Authors: Rozalina Rozalina Rozalina , Tusiah Tusiah 
Published: December 2019

Faktor-Faktor Yang Mempengaruhi Respon Petani Terhadap Penggunaan Mesin Prontok (Power Thresher) Padi (Oryza sativa, L) Di Kecamatan Peunaron Kabupaten Aceh Timur. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh faktor luas lahan, pendidikan dan pengalaman terhadap respon petani dalam penggunaan mesin perontok padi (power thresher) di Kecamatan Peunaron Kabupaten Aceh Timur.
Hasil penelitian diperoleh persamaan regresi linear berganda diperoleh : Y =1,192 + 1,422 X1 + 1,126 X2 + 0,332 X3. Artinya jika pendidikan dan pengalaman tetap maka setiap penambahan luas lahan garapan 1 Ha akan menyebabkan penambahan skor respon petani dalam penggunaan mesin perontok padi sebesar 1,422 skor. Kemudian jika luas lahan garapan dan pengalaman petani tetap maka setiap penambahan pendidikan 1 tahun akan menyebabkan penambahan skor respon petani dalam penggunaan mesin perontok padi sebesar 1,126 skor. Jika luas lahan garapan dan pendidikan tetap maka setiap penambahan pengalaman petani 1 tahun akan menyebabkan penambahan skor respon petani dalam penggunaan mesin perontok padi sebesar 0,332 skor.
Hasil perhitungan koefisien determinasi menunjukkan bahwa besarnya R2 = 0,921. Ini berarti variasi terhadap naik turunnya respon petani dalam penggunaan mesin perontok padi (Y) dipengaruhi oleh luas lahan (X1), pendidikan (X2) dan pengalaman (X3) sebesar 92,10 % dan sisanya 7,90% lagi dipengaruhi oleh faktor lain yang tidak ikut diteliti dalam penelitian ini.
Hasil pengujian secara serempak diperoleh F cari = 121,182 > F tabel = 2,91 pada tingkat kepercayaan 95% (a = 0,05) dan F tabel = 4,49 pada tingkat kepercayaan 99% (a = 0,01), sehingga luas lahan garapan, pendidikan dan pengalaman petani secara serempak berpengaruh sangat nyata terhadap respon petani dalam penggunaan mesin perontok padi.
Hasil pengujian secara parsial untuk luas lahan garapan dapat dilihat bahwa t1 cari = 8,034 > t tabel = 1,6955 pada tingkat kepercayaan 95% (a = 0,05) dan t tabel = 2,4528 pada tingkat kepercayaan 99% (a = 0,01), sehingga luas lahan garapan secara parsial berpengaruh sangat nyata terhadap respon petani dalam penggunaan mesin perontok padi. Hasil pengujian secara parsial untuk pendidikan dapat dilihat bahwa t2 cari = 1.126,00 > t tabel = 1,6955 pada tingkat kepercayaan 95% (a = 0,05) dan t tabel = 2,4528 pada tingkat kepercayaan 99% (a = 0,01), sehingga pendidikan secara parsial berpengaruh sangat nyata terhadap respon petani dalam penggunaan mesin perontok padi. Hasil pengujian secara parsial untuk pengalaman dapat dilihat bahwa t3 cari = 11.066,67 > t tabel = 1,6955 pada tingkat kepercayaan 95% (a = 0,05) dan t tabel = 2,4528 pada tingkat kepercayaan 99% (a = 0,01), sehingga pengalaman secara parsial berpengaruh sangat nyata terhadap respon petani dalam penggunaan mesin perontok padi.
STRATEGI PEMBANGUNAN PERTANIAN DAN PEREKONOMIAN PEDESAAN MELALUI KEMITRAAN USAHA BERWAWASAN AGRIBISNIS
Authors: Gustiana Cut 
Published: December 2019

Strategi Pembangunan Pertanian dan Perekonomian Pedesaan Melalui Kemitraan Usaha Berwawasan Agribisnis. Tujuan penulisan Karya Ilmiah ini untuk (1) Membahas profil ekonomi pedesaan sebagai basis perencanaan program pengembangan, (2) Membahas strategi dasar pembangunan pedesaan berwawasan agribisnis, dan (3) Merumuskan kebijaksanaan pengembangan agribisnis secara koprehensif dan integratif. Proses pembangunan (ekonomi) suatu bangsa secara implisit mensyaratkan adanya transformasi pertanian tradisional menjadi pertanian maju atau modern. Dalam proses transformasi itulah pola partisipasi memainkan peranannya. Pertanian maju adalah pertanian yang berkemampuan untuk terus-menerus menyesuaikan diri dengan tantangan dan permintaan pasar yang senantiasa berubah. Kelembagaan kemitraan usaha agribisnis yang dapat mengeliminir berbagai kegagalan tersebut adalah kelembagaan usaha agribisnis terpadu, berupa keterpaduan antarpelaku (petani, pedagang, pengolah) dan produk (bahan baku berkualitas, penanganan pascapanen yang prima serta jaminan keamanan pangan). Model tersebut didukung oleh revitalisasi kelembagaan kelompok tani, penyuluhan pertanian, dan perusahaan mitra.
Influence of organizational culture, supervision and environmental work towards discipline of Cicau village employees
Authors: Hidayat Rahmat , Kusumah Dadang Heri 
Published: December 2019

Each organization is required to be able to optimize human resources and human resources who are able to work hard and have high productivity. Achieving high productivity is not an easy thing to do. An important factor for achieving high productivity is the implementation of work discipline from its employees. In order for the company's operational activities to run well, supervision is needed. The purpose of supervision is for employees to be careful in their work. Supervision is very important, because with the supervision, the employee is serious in working. In addition, the work environment plays an important role in humans who play a role in achieving success in human endeavor or work. The main basis for implementing the work environment is knowledge and attention to human behavior as a determining factor for organizational success. This study aims to determine the effect of organizational culture, supervision and work environment on village employee work discipline. This type of research is quantitative. For data collection includes observations, questionnaires, and literature studies. The analytical methods used are validity test, reliability test, classic assumption test, multiple linear analysis and hypothesis testing. Keywords: Organizational culture, Supervision, Work environment, and work discipline.
The quality of work-life and relation to organizational excellence at King Khalid University of the faculty members' point of view / Business School Case study
Authors: Al Yami Ahmed , Haif A, bin Wared Eman 
Published: December 2019

This study aimed to identify the quality of the career path and its relation to organizational excellence at King Khalid University in the Faculty of Business from the point of view of the faculty members by identifying the dimensions quality of work-life including (participation of decision making, training and development opportunities, and the balance between personal and work life, and to identify the level of organizational excellence through dimensions ( Excellence of leadership, excellence of the strategy, and excellence of organizational culture). The descriptive approach was used. The questionnaire was a research tool. It consisted of (29) paragraphs, distributed to the entire study community and then received 127 responses. The results of the study showed that the level of quality of career life is generally average, while training and development came in the first order, followed by the quality of participation in decision making, while the balance between work and personal life ranked last. The level of organizational excellence was generally high. Excellence in organizational culture was in the first order, followed by strategic excellence, and finally leadership excellence. The study showed a statistically significant relationship between the total degree of quality of the functional life and all dimensions of the organizational excellence variable. Finally, the study recommended that the faculty members should participate more, adopt training programs, pay attention to leadership methods that stimulate work and production, attract the distinguished human resources, the need to provide the quality of work life.
Pengaruh Aktivitas Merokok Terhadap Hasil Analisa Sperma Pada Kasus Infertilitas Pria di Makassar
Authors: Rusman Kadri 
Published: December 2019

Tujuan penelitian ini adalah untuk mengetahui pengaruh aktivitas merokok terhadap hasil analisa sperma pada pria perokok. Penelitian ini menggunakan data sekunder dari rekam medik hasil pemeriksaan analisa sperma pasien infertil pria di praktik Prof.dr.Randanan yang datang berobat dari bulan januari s.d desember 2015, sebanyak 182 pasien yang terdiri dari 91 pasien infertil primer perokok dan 91 pasien infertil primer tidak merokok.Data dianalisis secara statisktik dan diolah secara komputerisasi dengan menggunakan Program Microsoft Excel dan Program SPSS versi 20.0 for Windows. Hasil penelitian ini menunjukkan bahwa : Secara umum tidak ada hubungan bermakna antara kualitas sperma pada penderita infertilitas primer pria baik yang merokok maupun yang tidak merokok. Pada hasil analisa sperma penderita infertilitas primer pria, ada pengaruh yang signifikan antara kualitas sperma dalam hal ini motilistas a dengan lamanya masa infertilitas, yaitu semakin lama masa infertilitas maka motilitas a akan semakin berkurang. Pada hasil analisa sperma terdapat hubungan aktivitas merokok dengan kuantitas sperma, yaitu semakin lama masa merokok maka semakin berkurang volume sperma dari penderita infertilitas primer pria.