bluuebunny/crossref_metadata_2025_split huggingface dataset:
Indoor Finish Material Influence on Contamination, Transmission, and Eradication of Methicillin-Resistant Staphylococcus aureus (MRSA)
Authors: Harris Debra , Taylor Keyanna P. , Napierkowski Katie , Zechmann Bernd 
Published: September 2020

Objective: The purpose of this study was to evaluate environmental surface materials used in healthcare environments for material composition, methicillin-resistant Staphylococcus aureus (MRSA) viability, and a comparison of two disinfectants, a bleach germicidal cleaner and Decon7, a novel disinfectant. Background: Contaminated environmental surfaces have been associated with outbreaks of healthcare-associated illness (HAIs). One in every 20 patients in U.S. acute care hospitals acquire a healthcare-associated illness, leading to consequences such as elevated morbidity, mortality, and a decrease in quality of life. In the patient environment, MRSA can remain viable from hours to up to 14 days. Methods: Environmental surface materials were evaluated as new and worn. Material composition and properties were assessed to evaluate surface integrity and the influence on the disinfection of MRSA. Inoculated materials were used to assess MRSA viability over time and the efficacy of a manufacturer’s recommended cleaning and disinfection product compared to a novel disinfectant. Results: Environmental surface materials respond differently in appearance and roughness, when mechanically worn. When measuring MRSA survival, at 24 hr, MRSA colony forming unit (CFU) counts were reduced on the copper sheet surface and solid surface with cupric oxide. By 72 hr, all MRSA counts were zero. Bleach and the novel disinfectant were equally effective at disinfecting MRSA from all surface types. Conclusions: This study highlights a gap in knowledge about the impact of type and wear of environmental surface materials used in healthcare environments on contamination with epidemiologically important organisms. In conclusion, environmental surface material wear, properties, and cleaning and disinfection efficacy are important factors to consider when addressing HAIs.
Grguricite, CaCr2(CO3)2(OH)4.4H2O, a new alumohydrocalcite analogue
Authors: Rumsey Michael S. , Welch Mark D. , Spratt John , Kleppe Annette K. 
Published: September 2020

AbstractThe occurrence and characterisation of a new member of the dundasite group are reported. Grguricite, ideally CaCr2(CO3)2(OH)4⋅4H2O, is the Cr analogue of alumohydrocalcite, CaAl2(CO3)2(OH)4⋅4H2O and occurs as lilac crusts of very fine-grained crystalline aggregates in the Pb–Ba–V mineralisation found at the Adeghoual Mine, Mibladen, Morocco (32°46′0″N, 4°37′59″W). The identification was based upon a close match with the powder X-ray diffraction data for alumohydrocalcite, the confirmation of anion components identified by Raman spectroscopy and the cation composition determined by electron-probe microanalysis. The empirical formula based upon 14 oxygen atoms per formula unit is Ca0.84Pb0.03Cr1.65Al0.39Mg0.02(CO3)2(OH)4⋅4H2O, with carbonate, hydroxyl and water contents set to those of the alumohydrocalcite stoichiometry. The fine-grained nature of the crystals (c. 0.5 μm × 0.1 μm × 5 μm) precluded a single-crystal X-ray study and both density and optical determinations. Grguricite is triclinic with space group P${\bar 1}$. Unit-cell parameters refined from the powder diffraction data are: a = 5.724(2), b = 6.5304(9), c = 14.646(4) Å, α = 81.682(1), β = 83.712(2), γ = 86.365(2)°, V = 537.8(2) Å3 and Z = 2. The five strongest peaks in the powder pattern are [dhkl, Å (I/Imax)(hkl)]: 6.222(100)(011), 3.227(87)(020), 6.454(63)(010), 2.883(58)(005, 023, 121) and 7.208(45)(002). The mineral is named after Australian geologist Ben Grguric.
Applying health, safety, and environmental risk assessment at academic settings
Authors: Dehdashti Alireza , Fatemi Farin , Jannati Muhammadreza , Asadi Fatemeh , Kangarloo Marzieh Belji 
Published: September 2020

Abstract
Background
Students, staff, and faculties are involved in activities that exposed them to a range of minor to severe or even fatal accidents in academic settings. Managing work environment risks is crucial to any safety and health prevention program. This study developed a risk assessment using combinations of hazards and risk factors to establish a scale of measures in a risk reduction action plan.

Methods
This cross-sectional study was conducted in an Iranian medical sciences university in 2018. A structured method of risk assessment was developed, applying a three-step procedure to identify hazards, consequences, and risk evaluation. Data were collected through detailed health, safety, and environment checklist in 38 different sites. Finally, the risks quantified, prioritized, and control measures proposed accordingly. Chi-square and correlation tests assessed how environmental factors were associated with hazard consequences. The analysis results were evaluated at the significance level of 0.05.

Results
The frequencies of moderate and high-risk levels were 22.7 and 2.9%, respectively. Thus, corrective measures should be considered as soon as possible and immediately for these risk groups. Facilities and functions within laboratories, library, and powerhouse were more vulnerable to serious risks. The type of hazard had associated with the sites and total risk score at the significance level of 0.05 (P-value = 0.017). Similarly, risk severity was significantly related to the sites (P-value = 0.003). Safety hazards had a statistically higher contribution to the total risk score when compared to health and environmental hazards.

Conclusion
The study revealed complex risks and hazardous circumstances with significant variances in academic sites and activities. Universities should provide training in risk reduction programs to increase the awareness of students, staff, and faculties, which can improve life safety in a university environment.
Prevalence of work-related musculoskeletal symptoms and associated risk factors among domestic gas workers and staff of works department in Enugu, Nigeria: a cross-sectional study
Authors: Oluka Chinenye Doris , Obidike Esther , Ezeukwu Antoninus Obinna , Onyeso Ogochukwu Kelechi , Ekechukwu Echezona Nelson Dominic 
Published: September 2020

AbstractBackgroundThe impact of work-related musculoskeletal symptoms (WMSS) permeates various occupations.ObjectiveTo compare WMSS and associated risk factors among domestic gas workers (DGWs) and staff of Works Department (SWD) in Enugu.MethodsOne-hundred adults (DGW = 50, SWD = 50) participated in this cross-sectional study. The Nordic Musculoskeletal Questionnaire and a demographics questionnaire were used to assess the prevalence of WMSS and related risk factors. Data were analysed using independentt-test or Mann-Whitney U, chi-square, and logistic regression atp < 0.05.ResultsThe DGWs (86%) had a significantly (χ2 = 24.45,p < 0.001) higher WMSS than the SWD (38%). Lower-back (54%) and shoulder (52%) were the most affected body parts among the DGWs in comparison to the hips/thighs (20%) among the SWD. Work-related factors such as daily work-duration (χ2 = 75.44,p < 0.001), lifting training (χ2 = 96.24,p < 0.001), and use of personal protective equipment (PPE) of facemask (χ2 = 100.0,p < 0.001) and gloves (χ2 = 96.09,p < 0.001) were significantly associated with general WMSS among the DGWs. However, diastolic blood pressure (DBP) (OR = 1.29,p = 0.018), work duration > 8 h/day (OR = 0.001,p = 0.028), female gender (OR = 6.98–10.26,p < 0.05), sleep duration < 6 h/day (OR = 0.56–0.73,p < 0.05) and poor exercise behaviour (OR = 0.15,p = 0.013) were the identified independent risk factors of WMSS among DGWs, while DBP (OR = 0.99,p = 0.012) and female gender (OR = 6.47,p = 0.032) were the only identified independent risk factors for SWD.ConclusionWMSS is significantly higher among DGWs than the SWD. High DBP, female gender, working beyond 8 h per day, sleeping less than 6 h per day, and insufficient exercise increase the risks of WMSDs, especially among the DGWs. To mitigate the adverse effects of WMSDs, SWD and DGWs require break and leave periods, PPE and assistive devices, exercise, medical check-up, and workplace ergonomics.
Longitudinal observation of solitary fibrous tumor translation into malignant pulmonary artery intimal sarcoma
Authors: Luo Rui , Jiang Yingshuo , Huang Yue , Chen Xin , Wang Feng 
Published: September 2020

Abstract
Background
Pulmonary artery intimal sarcoma (PAIS) is a rare malignant tumor that was usually misdiagnosed as chronic pulmonary thromboembolism.

Case presentation
We previously reported a solitary fibrous tumor in the pulmonary artery presented with acute pulmonary embolism, which was identified by 99mTc-Galacto-RGD2 imaging. However, this patient had a recurrence in situ two-year after surgery, post-operative pathology revealed pulmonary artery intimal sarcoma. At one-year post-operation, 18F-FDG PET/CT was performed for exclusion of tumor metastasis, which showed FDG avid lesion in the T5, T10, and L5 vertebral bodies, as well as in bilateral ilium and right ischium.

Conclusions
This is the first longitudinal observation of a solitary fibrous tumor (SFT) development into a pulmonary artery intimal sarcoma (PAIS) and presented with multiple bone metastases.
Chidamide, decitabine, cytarabine, aclarubicin, and granulocyte colony-stimulating factor (CDCAG) in patients with relapsed/refractory acute myeloid leukemia: a single-arm, phase 1/2 study
Authors: Wang Lixin , Luo Jianmin , Chen Guofeng , Fang Meiyun , Wei Xudong , Li Yinghua , Liu Zhuogang , Zhang Yin , Gao Sujun , Shen Jianliang , Wang Xin , Gao Xiaoning , Zhou Wei , Ma Yigai , Liu Hui , Li Xinquan , Yang Linhua , Sun Kai , Yu Li 
Published: September 2020

Abstract
Background
Epigenetic mechanisms play an important role in the chemoresistance of acute myeloid leukemia (AML). The clinical response to epigenetic modifier-based chemotherapy in patients with relapsed/refractory AML (r/r AML) is unclear. This multicenter clinical trial evaluated the safety and efficacy of epigenetic modifiers (chidamide and decitabine) in combination with aclarubicin, cytarabine, and granulocyte colony-stimulating factor (G-CSF) in patients with r/r AML.

Results
Adult patients with r/r AML were treated with chidamide, decitabine, cytarabine, aclarubicin, and G-CSF (CDCAG). The primary measures were overall response (OR), overall survival (OS), and safety. Next-generation sequencing was performed to analyze the correlation between gene mutations and response. A total of 93 patients with r/r AML were enrolled. Overall, 24 patients had a complete remission (CR) and 19 patients achieved CR with incomplete blood count recovery (CRi). The overall response rate (ORR) was 46.2%. The overall survival of these 43 patients who achieved CR/CRi was significantly longer than that of patients who failed to achieve remission (563 vs 152 days, P < 0.0001). Of the patients with mutations in epigenetic and transcription factor-related genes, but without internal tandem duplications in FMS-like tyrosine kinase3 (FLT3-ITDs), 55.6% achieved CR/CRi, whereas the ORR was 28.2% for patients with mutations in other genes.

Conclusions
The CDCAG regimen was well tolerated and effective in r/r AML. Patients with epigenetic and transcription factor-related gene mutations, but without FLT3-ITD mutations, may benefit from this regimen.

Trial registration
Clinical Trials, NCT02886559. Registered 01 September 2016
Autism-associated variants of neuroligin 4X impair synaptogenic activity by various molecular mechanisms
Authors: Yumoto Takafumi , Kimura Misaki , Nagatomo Ryota , Sato Tsukika , Utsunomiya Shun , Aoki Natsue , Kitaura Motoji , Takahashi Koji , Takemoto Hiroshi , Watanabe Hirotaka , Okano Hideyuki , Yoshida Fumiaki , Nao Yosuke , Tomita Taisuke 
Published: September 2020

Abstract
Background
Several genetic alterations, including point mutations and copy number variations in NLGN genes, have been associated with psychiatric disorders, such as autism spectrum disorder (ASD) and X-linked mental retardation (XLMR). NLGN genes encode neuroligin (NL) proteins, which are adhesion molecules that are important for proper synaptic formation and maturation. Previously, we and others found that the expression level of murine NL1 is regulated by proteolytic processing in a synaptic activity-dependent manner.

Methods
In this study, we analyzed the effects of missense variants associated with ASD and XLMR on the metabolism and function of NL4X, a protein which is encoded by the NLGN4X gene and is expressed only in humans, using cultured cells, primary neurons from rodents, and human induced pluripotent stem cell-derived neurons.

Results
NL4X was found to undergo proteolytic processing in human neuronal cells. Almost all NL4X variants caused a substantial decrease in the levels of mature NL4X and its synaptogenic activity in a heterologous culture system. Intriguingly, the L593F variant of NL4X accelerated the proteolysis of mature NL4X proteins located on the cell surface. In contrast, other variants decreased the cell-surface trafficking of NL4X. Notably, protease inhibitors as well as chemical chaperones rescued the expression of mature NL4X.

Limitations
Our study did not reveal whether these dysfunctional phenotypes occurred in individuals carrying NLGN4X variant. Moreover, though these pathological mechanisms could be exploited as potential drug targets for ASD, it remains unclear whether these compounds would have beneficial effects on ASD model animals and patients.

Conclusions
These data suggest that reduced amounts of the functional NL4X protein on the cell surface is a common mechanism by which point mutants of the NL4X protein cause psychiatric disorders, although different molecular mechanisms are thought to be involved. Furthermore, these results highlight that the precision medicine approach based on genetic and cell biological analyses is important for the development of therapeutics for psychiatric disorders.
CompRet: a comprehensive recommendation framework for chemical synthesis planning with algorithmic enumeration
Authors: Shibukawa Ryosuke , Ishida Shoichi , Yoshizoe Kazuki , Wasa Kunihiro , Takasu Kiyosei , Okuno Yasushi , Terayama Kei , Tsuda Koji 
Published: September 2020

AbstractIn computer-assisted synthesis planning (CASP) programs, providing as many chemical synthetic routes as possible is essential for considering optimal and alternative routes in a chemical reaction network. As the majority of CASP programs have been designed to provide one or a few optimal routes, it is likely that the desired one will not be included. To avoid this, an exact algorithm that lists possible synthetic routes within the chemical reaction network is required, alongside a recommendation of synthetic routes that meet specified criteria based on the chemist’s objectives. Herein, we propose a chemical-reaction-network-based synthetic route recommendation framework called “CompRet” with a mathematically guaranteed enumeration algorithm. In a preliminary experiment, CompRet was shown to successfully provide alternative routes for a known antihistaminic drug, cetirizine. CompRet is expected to promote desirable enumeration-based chemical synthesis searches and aid the development of an interactive CASP framework for chemists.
An open source chemical structure curation pipeline using RDKit
Authors: Bento A. Patrícia , Hersey Anne , Félix Eloy , Landrum Greg , Gaulton Anna , Atkinson Francis , Bellis Louisa J. , De Veij Marleen , Leach Andrew R. 
Published: September 2020

Abstract
Background
The ChEMBL database is one of a number of public databases that contain bioactivity data on small molecule compounds curated from diverse sources. Incoming compounds are typically not standardised according to consistent rules. In order to maintain the quality of the final database and to easily compare and integrate data on the same compound from different sources it is necessary for the chemical structures in the database to be appropriately standardised.

Results
A chemical curation pipeline has been developed using the open source toolkit RDKit. It comprises three components: a Checker to test the validity of chemical structures and flag any serious errors; a Standardizer which formats compounds according to defined rules and conventions and a GetParent component that removes any salts and solvents from the compound to create its parent. This pipeline has been applied to the latest version of the ChEMBL database as well as uncurated datasets from other sources to test the robustness of the process and to identify common issues in database molecular structures.

Conclusion
All the components of the structure pipeline have been made freely available for other researchers to use and adapt for their own use. The code is available in a GitHub repository and it can also be accessed via the ChEMBL Beaker webservices. It has been used successfully to standardise the nearly 2 million compounds in the ChEMBL database and the compound validity checker has been used to identify compounds with the most serious issues so that they can be prioritised for manual curation.
Non-destructive collection survey of the historical Classense Library. Part I: Paper characterisation
Authors: Brown Natalie , Coppola Floriana , Modelli Alberto , Amicucci Floriana , Lichtblau Dirk , Strlič Matija 
Published: September 2020

Abstract
An innovative survey was conducted of the collections of the historical Biblioteca Classense, located in the urban area of Ravenna (Northern Italy). The survey aimed to evaluate the current conservation state of the book collections, where 297 paper-based items, including incunabula, manuscripts and books, dating from the 14th to the 20th century, were selected for analysis. This innovative survey was carried out non-destructively by assessing degradation visually and by measuring NIR spectral data followed by multivariate data analysis. Chemical and physical paper properties, important for paper characterisation and implementation of conservation strategies were determined, including paper type, pH, degree of polymerisation (DP), tensile strength, lignin, protein, and rosin content. This survey provided a significant quantitative dataset for rag paper covering a 600-year period. The analysis of DP changes over time allowed the first experimental estimation of the rate constant for historical rag paper, i.e., (4.2 ± 0.6)·10−7 year−1, which was validated with predictions based on the Collections Demography dose response function for historic paper taking into account the past climate in Ravenna. Statistical methods were employed to describe the correlations between the measured variables and different features of the books, suggesting that the degree of polymerisation can be used as a general proxy for rag paper mechanical strength.
Perception, pattern of use, partner support and determinants of uptake of family planning methods among women in rural communities in Southeast Nigeria
Authors: Akamike Ifeyinwa Chizoba , Madubueze Ugochukwu Chinyem , Okedo-Alex Ijeoma Nkem , Anyigor Chika Julius , Azuogu Benedict Ndubueze , Umeokonkwo Chukwuma David , Mbachu Chinyere Ojiugo 
Published: September 2020

Abstract
Background
Family planning is a cost-effective strategy for achieving population development. Family planning uptake is low in sub-Saharan Africa, including Nigeria. We assessed the perception, pattern of use, partner support and determinants of uptake of family planning methods among married women of reproductive age in rural communities of Ebonyi state.

Methods
This is part of a baseline report of a quasi-experimental study. A total of 484 married women of reproductive age were recruited using multistage sampling method. Four focus group discussions (men and women) and pre-tested semi-structured interviewer-administered questionnaires were used to collect information from the participants. Data were analysed using Statistical Package for Social Sciences (SPSS) version 20 software and thematic analysis. Chi-square test and logistic regression were carried out at 5% significance level.

Results
Only 26.2% of respondents were currently using any method of family planning. The most commonly used method was the natural method (57%). Amongst those who reported using artificial methods, 32.7% used condoms, 27.3% used implant while 23.64 and 16.4% used injectables and pills respectively. Predictors of current use of any family planning method were: older age (AOR = 1.7, 95%CI = 1.01–3.00), having more than five children (AOR = 1.7, 95%CI = 1.05–2.83), minimum of secondary level of education for respondent (AOR = 3.3, CI = 1.60–6.96) and their husband/partner (AOR = 2.0, 95%CI = 1.05–3.92). Qualitative findings showed that only few families were using a method of family planning and those who did not practice family planning perceived it to interfere with God’s plan for fruitfulness and to be counter-productive to household income due to decreased manpower for agricultural activities. Poor partner involvement and support for family planning was also cited as a deterrent by both male and female participants.

Conclusions
Perception and use of family planning methods is poor in rural communities of Ebonyi state. Improving uptake of family planning methods in these rural communities will require proper demographic targeting as well as debunking fatalistic views, and cultural and religious myths around family planning.
Massive pulmonary thromboembolism combined with transient thyrotoxicosis in an 18 year old girl
Authors: Kim Tong-Yoon , Ihm Sang-Hyun , Roh Ji Woong , Lim Sungmin , Park Chan-Seok , Kim Hee-Yeol 
Published: September 2020

AbstractBackgroundPulmonary thromboembolism (PTE) is thought to usually stem from deep vein thrombosis (DVT). However, evidence of DVT could not be found in many cases. Furthermore, transient thyrotoxicosis is a rare but potentially life–threatening emergency involving a systemic hypercoagulable state. We report on an 18 year-old-girl with transient thyrotoxicosis with massive PTE without DVT.Case presentationAn 18-year-old girl was admitted to the hospital with syncope. Patient had no history of trauma, any known underlying disease or oral contraceptives use. Chest computed tomography (CT) showed massive PTE in both central pulmonary arteries and diffuse goiter. However, a low extremity Doppler sonogram did not detect DVT. To manage the PTE, we administered low molecular weight heparin. On the other hands, thyroid function test indicated a state of thyrotoxicosis. In addition, patient had a partial protein S deficiency but no other immunologic abnormality. Therefore, the patient was diagnosed with massive PTE, thyrotoxicosis, and partial protein S deficiency. Patient was discharged with oral warfarin and methimazole. A follow-up echocardiogram obtained 3 months after anticoagulation therapy demonstrated normal dimensions and systolic function. After thyrotoxicosis was treated with methimazole for a month, a euthyroid state was achieved and the goiter decreased to a normal size. The methimazole was gradually tapered off and stopped at 4 months. At a 6-month follow up visit, PTE and pulmonary hypertension had disappeared but the patient still had a partial protein S deficiency. We decided to stop all medication with careful monitoring. During a 4-year follow-up period after the episode, she was asymptomatic without any evidence of recurrent systemic thromboembolism or hyperthyroidism.ConclusionsEarly recognition and appropriate treatment of PTE combined with transient thyrotoxicosis were vital to preventing other complications.