bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbial extraction of chitin from seafood waste using sugars derived from fruit waste-stream
Authors: Tan Yun Nian , Lee Pei Pei , Chen Wei Ning 
Published: January 2020

AbstractChitin and chitosan are natural amino polysaccharides that have exceptional biocompatibility in a wide range of applications such as drug delivery carriers, antibacterial agents and food stabilizers. However, conventional chemical extraction methods of chitin from marine waste are costly and hazardous to the environment. Here we report a study where shrimp waste was co-fermented with Lactobacillus plantarum subsp. plantarum ATCC 14917 and Bacillus subtilis subsp. subtilis ATCC 6051 and chitin was successfully extracted after deproteinization and demineralization of the prawn shells. The glucose supplementation for fermentation was replaced by waste substrates to reduce cost and maximize waste utilization. A total of 10 carbon sources were explored, namely sugarcane molasses, light corn syrup, red grape pomace, white grape pomace, apple peel, pineapple peel and core, potato peel, mango peel, banana peel and sweet potato peel. The extracted chitin was chemically characterized by Fourier Transform Infrared Spectroscopy (FTIR) to measure the degree of acetylation, elemental analysis (EA) to measure the carbon/nitrogen ratio and X-ray diffraction (XRD) to measure the degree of crystallinity. A comparison of the quality of the crude extracted chitin was made between the different waste substrates used for fermentation and the experimental results showed that the waste substrates generally make a suitable replacement for glucose in the fermentation process. Red grape pomace resulted in recovery of chitin with a degree of deacetylation of 72.90%, a carbon/nitrogen ratio of 6.85 and a degree of crystallinity of 95.54%. These achieved values were found to be comparable with and even surpassed commercial chitin.
Dialysis membrane enclosed laccase catalysis combines a controlled conversion rate and recyclability without enzyme immobilization
Authors: Zhang Jie , Li Fukun , Wang Ruiqi , Tan Xuemei , Hagedoorn Peter-Leon 
Published: January 2020

AbstractLaccase is a versatile multicopper oxidase that holds great promise for many biotechnological applications. For such applications, it is essential to explore good biocatalytic systems for high activity and recyclability. The feasibility of membrane enclosed enzymatic catalysis (MEEC) for enzyme recycling with laccase was evaluated. The dialysis membrane enclosed laccase catalysis (DMELC) was tested for the conversion of the non-phenolic model substrate 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS). Trametes versicolor laccase was found to be completely retained by the dialysis membrane during the process. The ABTS total conversion after DMELC reached the same values as the batch reaction of the enzyme in solution. The efficiency of DMELC conversion of ABTS under different process conditions including shaking speed, temperature, ABTS concentration and pH was investigated. The repetitive dialysis minimally affected the activity and the protein content of the enclosed laccase. DMELC retained 70.3 ± 0.8% of its initial conversion after 5 cycles. The usefulness of MEEC extends to other enzymes with the benefit of superior activity of an enzyme in solution and the recyclability which is normally only obtained with immobilized enzymes.
Bactericidal activity of Myrrh extracts and two dosage forms against standard bacterial strains and multidrug-resistant clinical isolates with GC/MS profiling
Authors: Khalil Noha , Fikry Sahar , Salama Osama 
Published: January 2020

AbstractMyrrh is the resinous exudate obtained by the incision in Commiphora molmol trees (Family Burseraceae). The bactericidal activity of its hexane extract was compared to its essential oil (MEO) using viable count technique against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (Ps. aeruginosa). MEO exhibited a better activity with > 99.999% killing of both tested strains after 2 h contact time. MEO was tested using the same technique against four multidrug resistant isolates: S. aureus (MRSA, sputum), Escherichia coli (E. coli, urine), Ps. aeruginosa (wound) and Klebsiella pneumonia (K. pneumonia, sputum). Highest bactericidal activity was observed against Ps. aeruginosa while lowest was against K. pneumonia (99.59 and 54.04% killing, respectively after 2 h contact time). A cream and mouthwash were formulated using 5% v/v MEO. The cream showed a better activity against Ps. aeruginosa than S. aureus (95.11 and 86.76% killing, respectively after 2 h contact time). The in vitro treatment of ca 107 CFU/ml S. aureus cells suspended in 10% saliva with the mouthwash produced ca 46% killing within the first 15 min reaching ca 99.999% after 30 min. Cytotoxic studies of both the essential oil and hexane extract on human liver cancer (Hep G2), human breast cancer (MCF-7) and colon cancer cell lines (HCT-116) revealed a promising in vitro activity. Highest activity was recorded for the essential oil on MCF-7 with IC50 10.93 ± 0.32 μg/ml. GC/MS analysis allowed the identification of 17 and 9 compounds representing 92.01 and 99.99% of the hexane extract and essential oil, respectively. Furano-eudesma-1,3-diene (15.99%) and 2-acetoxy-furano-diene (26.82%) were the major identified compounds in the hexane extract and essential oil, respectively. These results indicate that Myrrh essential oil is a promising antibacterial and cytotoxic agent that can be formulated in suitable dosage forms.
Van Wyk-Grumbach syndrome in a female pediatric patient with trisomy 21: a case report
Authors: Gupta Jyotsna , Lin-Su Karen 
Published: January 2020

Abstract
Background
Children with hypothyroidism typically present with delayed growth and development, but on rare occasions can present with signs of precocious puberty. This presentation is called Van Wyk-Grumbach syndrome. Van Wyk-Grumbach syndrome has seldom been described in patients with trisomy 21.

Case presentation
We present the case of a 4-year-old girl with trisomy 21, who recently moved to the United States from Guyana, and presented to the emergency room with recurrent vaginal bleeding. She was eventually diagnosed with hypothyroidism and Van Wyk-Grumbach syndrome. She was noted to have Tanner I breasts and pubic hair. A pelvic ultrasound was performed, which showed a simple cyst in the right adnexa. Subsequent laboratory evaluation revealed a thyroid stimulating hormone (TSH) of > 150 mIU/ml along with low free thyroxine of 0.3 ng/dl, suggesting longstanding untreated hypothyroidism. Estradiol and alpha-fetoprotein (AFP) levels were elevated. Bone age was delayed. The patient was diagnosed with Van Wyk-Grumbach syndrome and was started on levothyroxine therapy with subsequent resolution of vaginal bleeding. Estradiol and AFP both normalized after initiating levothyroxine therapy.

Conclusion
This case emphasizes the importance of recognizing the presence of precocious puberty, delayed bone age and ovarian cyst as a manifestation of primary hypothyroidism. In addition, it highlights the need for thyroid function screening in patients with Trisomy 21. Tumor markers may be elevated in Van Wyk-Grumbach syndrome with subsequent normalization after treatment.
The aerodynamics of voiced stop closures
Authors: Jesus Luis M. T. , Costa Maria Conceição 
Published: January 2020

AbstractExperimental data combining complementary measures based on the oral airflow signal is presented in this paper, exploring the view that European Portuguese voiced stops are produced in a similar fashion to Germanic languages. Four Portuguese speakers were recorded producing a corpus of nine isolated words with /b, d, ɡ/ in initial, medial and final word position, and the same nine words embedded in 39 different sentences. Slope of the stop release (SLP), voice onset time (VOT), release and stop durations and steady-state oral airflow amplitude characteristics preceding and following the stop were analysed. Differences between independent groups (three different places of articulation and two vowel contexts) and correlations between variables were studied; generalised linear mixed effects models were developed to study the effects of VOT, SLP and the factors place of articulation and vowel context on the mean oral airflow. A classification of stop’s voicing was automatically extracted. Both SLP (p= .013) and VOT (p= .014) were significantly different for the three places of articulation. Weak voicing was observed for 57% of the stops. It is hypothesised that the high percentages of weakly voiced stops are a consequence of passive voicing and that the feature of contrast in Portuguese is privative [spread glottis].
Investigation of post-therapeutic image-based thyroid dosimetry using quantitative SPECT/CT, iodine biokinetics, and the MIRD’s voxel S values in Graves’ disease
Authors: Fujita Naotoshi , Koshiba Yumiko , Abe Shinji , Kato Katsuhiko 
Published: January 2020

Abstract
Background
Before radioiodine therapy for Graves’ disease, the estimated thyroid-absorbed dose is calculated based on various clinical parameters. However, the actual accumulation of iodine in the thyroid during radioiodine therapy is not determined. We validated the feasibility of post-therapeutic image-based thyroid dosimetry through quantitative single-photon emission computed tomography (SPECT) imaging and thyroid biokinetics and expanding the Medical Internal Radiation Dose Committee’s (MIRD) voxel dosimetry guidelines.

Methods
Forty-three patients with Graves’ disease who underwent radioiodine therapy were chosen as subjects for this retrospective analysis. We acquired patients’ SPECT images 24 h after oral administration. SPECT images were quantified using system volume sensitivity to calculate time-integrated activity coefficients on a voxel basis. Absorbed dose was obtained by convolving MIRD guideline voxel S values with time-integrated activity coefficients. To determine accuracy, we compared the results obtained using the post-therapeutic image-based absorbed-dose method (D̅image,PVC) with absorbed doses calculated using the method described by the European Association of Nuclear Medicine (pre-therapeutic method; DEANM).

Results
Using image-based dosimetry as post-therapeutic dosimetry, we visualized the local accumulation and absorbed dose distribution of iodine in the thyroid. Furthermore, we determined a strong correlation (Pearson’s correlation coefficient = 0.89) between both dosimetries, using the regression equation: D̅image,PVC = 0.94 × DEANM + 1.35.

Conclusion
Post-therapeutic image-based doses absorbed in the thyroid resembled those of pre-therapeutic EANM method-based absorbed doses. Additionally, the post-therapeutic image-based method had the advantage of visualizing thyroid iodine distribution, thus determining local dose distributions at the time of treatment. From these points, we propose that post-therapeutic image-based dosimetry could provide an alternative to standard pre-therapeutic dosimetry to evaluate dose response.
Arterial communication around the pancreatic tail enabled division of the gastroduodenal artery during pancreaticoduodenectomy in patient with complete celiac artery occlusion: a case report
Authors: Oikawa Ryo , Ito Kyoji , Takemura Nobuyuki , Mihara Fuminori , Kokudo Norihiro 
Published: January 2020

Abstract
Background
Stenosis or obstruction of the celiac artery (CA) is known as celiac artery stenosis (CAS) and is usually accompanied by the formation of arterial anastomosis between the superior mesenteric artery (SMA) system and the CA system. Arterial bypass is mainly achieved through the gastroduodenal artery (GDA); therefore, the division of the GDA during pancreaticoduodenectomy (PD) could pose a problem in patients with CAS.

Case presentation
We reported a case of PD presenting complete occlusion of the CA, in which perfusion to organs in the CA system was maintained via peri-pancreatic arterial communication. There were complicated arterial anastomoses around the pancreas, which were clearly visualized on a three-dimensional reconstruction of the arterial system using multi-detector computed tomography. Among these complicated anastomoses, one well-developed anastomosis between the SMA and the splenic artery through the dorsal pancreatic artery (DPA) was identified. The DPA was considered to work as a potential collateral pathway from the SMA to organs in the CA system after division of the GDA. During surgery, Doppler ultrasonography detected hepatopetal arterial flow even after the GDA clamping; therefore, we performed typical PD with division of the GDA. The postoperative course of the patient was uneventful, and there was no sign of ischemic complications in the CA system organs including the liver, stomach or spleen.

Conclusions
Three-dimensional reconstruction of the arterial system using multi-detector computed tomography and the intraoperative GDA clamping test were useful to determine whether it was possible to divide the GDA in PD, in the case of CAS.
A case of resected primary pulmonary pleomorphic carcinoma with long-term survival after multidisciplinary treatment
Authors: Iijima Yoshihito , Nakajima Yuki , Kinoshita Hiroyasu , Kurihara Yasuyuki , Nishimura Yu , Iizuka Toshihiko , Akiyama Hirohiko , Hirata Tomomi 
Published: January 2020

Abstract
Background
Generally, primary pulmonary pleomorphic carcinoma is resistant to treatment and has a poor prognosis. We report a case of resected primary pulmonary pleomorphic carcinoma with long-term survival after multidisciplinary treatment.

Case presentation
A 74-year-old man with a history of emphysema, pneumoconiosis, and chronic bronchitis presented with left lung nodule and left adrenal tumor based on computed tomography. We suspected clinical T1bN0M1b, stage IVB lung cancer. Adrenalectomy of the left adrenal tumor yielded a definitive diagnosis of pleomorphic carcinoma. Chemotherapy was performed despite the spontaneous regression of lung lesions. Since lung lesions re-enlarged 11 months after adrenalectomy, the left lower lobe was partially resected followed by chemotherapy. The lung lesion was the primary lesion of the adrenal tumor. There was no recurrence 100 months after the lung resection.

Conclusions
The patient experienced long-term survival after multidisciplinary treatment. Both multidisciplinary treatment and immunological mechanisms caused spontaneous regression of the primary lesion.
The Rate of Infectious Complications After Intrathecal Drug Delivery System Implant for Cancer-Related Pain Is Low Despite Frequent Concurrent Anticancer Treatment or Leukopenia
Authors: Sindt Jill E. , Larsen Steven D. , Dalley Andrew P. , Collier Willem H. , Brogan Shane E. 
Published: January 2020

BACKGROUND:
The efficacy of intrathecal drug delivery (IDD) for cancer-related pain is well established. Cancer therapies are often associated with immunosuppression and increased risk of infection, and the rate of infection after intrathecal drug delivery system (IDDS) implant in cancer patients has been reported as 2.4%–6.3%. Our objective is to report on the rate of surgical site infections (SSI) in patients implanted with IDDS for cancer-related pain and to provide a data-driven discussion on the relationship between antineoplastic treatment, leukopenia, and other clinical or demographic characteristics and SSI.

METHODS:
Following local institutional review board approval, we conducted a retrospective chart review of IDDS implants from May 2014 through December 2018. Data collected included demographic data, health status, prophylactic antibiotic administration, surgery duration, presence of leukopenia (white blood cell [WBC] count of <4.0 K/µL) or moderate neutropenia (absolute neutrophil count [ANC] of <1000/μL) within the 30 days before IDDS implant, and details of antineoplastic treatment or systemic corticosteroid use in the perioperative period. This information was assessed in relation to SSI incidence up to 6 months following implant.

RESULTS:
Two hundred seventeen IDDS implants were identified. A majority of patients (79.3%) received ≥1 form of antineoplastic therapy within 30 days before or after implant, and 42.4% received multiple forms of antineoplastic therapy. Therapies included chemotherapy in 46.5%, immunotherapy in 28.6%, systemic steroids in 32.3%, and radiation therapy in 28.1%. One-quarter of patients (25.8%) were leukopenic within 30 days before implant, with 3.2% having moderate neutropenia. There were 2 infectious complications representing an infection rate of 0.9% (95% CI, 0.1%–3.3%), with limited shared characteristics between those experiencing SSI.

CONCLUSIONS:
SSI risk after IDDS placement for cancer pain is low, despite frequent concurrent antineoplastic therapy and leukopenia in the perioperative period. Concomitant cancer therapies should not be a barrier to the implementation of IDD for cancer pain.
Differential Regulation of the Glucocorticoid Receptor in a Rat Model of Inflammatory Pain
Authors: Ibrahim Shaimaa I. A. , Strong Judith A. , Qualls Katherine A. , Ulrich-Lai Yvonne M. , Zhang Jun-Ming 
Published: January 2020

BACKGROUND:
Anti-inflammatory corticosteroids are a common treatment for different conditions involving chronic pain and inflammation. Clinically used steroids target the glucocorticoid receptor (GR) for its anti-inflammatory effects. We previously reported that GR in sensory neurons may play central roles in some pain models and that GR immunoreactivity signal in dorsal root ganglia (DRG) decreased after local inflammation of the DRG (a model of low back pain). In the current study, we aimed to determine if similar changes in GR signal also exist in a skin inflammation model, the complete Freund’s adjuvant (CFA) model (a model of peripheral inflammatory pain), in which the terminals of the sensory neurons rather than the somata are inflamed.

METHODS:
A low dose of CFA was injected into the hind paw to establish the peripheral inflammation model in Sprague-Dawley rats of both sexes, as confirmed by measurements of behavior and paw swelling. Immunohistochemical and western blotting techniques were used to determine the expression pattern of the GR in the inflamed hind paw and the DRGs. Plasma corticosterone levels were measured with radioimmunoassay.

RESULTS:
The immunohistochemical staining revealed that GR is widely expressed in the normal DRG and skin tissues. Paw injection with CFA caused upregulation of the GR in the skin tissue on postinjection day 1, mostly detected in the dermis area. However, paw inflammation significantly reduced the GR signal in the L5 DRG 1 day after the injection. The GR downregulation was still evident 14 days after CFA inflammation. On day 1, western blotting confirmed this downregulation and showed that it could also be observed in the contralateral L5 DRG, as well as in the L2 DRG (a level which does not innervate the paw). Plasma corticosterone levels were elevated in both sexes on day 14 after CFA compared to day 1, suggesting autologous downregulation of the GR by corticosterone may have contributed to the downregulation observed on day 14 but not day 1.

CONCLUSIONS:
There are distinctive patterns of GR activation under different pain conditions, depending on the anatomical location. The observed downregulation of the GR in sensory neurons may have a significant impact on the use of steroids as treatment in these conditions and on the regulatory effects of endogenous glucocorticoids.
Sudan after Revolt
Authors: Elsheikh Elsadig 
Published: January 2020

Abstract
After more than four months of constant demonstrations, the Sudanese popular uprising, begun on December 19, 2018, and led by the Sudanese Professionals Association (SPA), forced the high-ranking generals of the Sudanese Armed Forces to side with the protestors in removing the dictatorial regime of Omar al-Bashir on April 11, 2019. The SPA developed new tactics for organizing and mobilizing the masses in the face of crippling public fear to put an end to thirty years of al-Bashir's dictatorship. However, the peaceful revolution has yet to declare its final victory and finds itself facing a major enemy, the Transitional Military Council (TMC), which intends to maintain the status quo ante. This essay asks: can Sudanese civil society challenge the TMC and lead the country out of autocracy, achieving genuine democratization, or will the TMC cut short such transformation?
PAN
Authors: Taylor Jane 
Published: January 2020

Abstract
In this text, the script of a performance/lecture, which combines live puppetry, digital film, and a lecture, is paired with a prefatory essay that seeks to address the theoretical questions raised by the play about embodiment, mind, AI, and the staging of consciousness. The play was performed at the Centre for the Less Good Idea, an arts laboratory, in Maboneng, Johannesburg, South Africa, in October 2018. In the play, two characters stand in as the early pioneers of primate research, Wolfgang Köhler (an early Gestalt psychologist) and Jane Goodall, whose observational fieldwork shifted primate studies profoundly. These two distinctive intellects advanced the commitment of the human species to work toward the preservation of, and engagement with, higher primates and in such ways altered our apprehension of the limits of the human through a challenge posed by our closest nonhuman kin. The play also explores the research of Norbert Wiener, the pioneer of the field of cybernetics (a term that he invented). Wiener inaugurated the massive proliferation of research into “feedback” theory, which he saw as fundamental to mechanic intelligence. In such terms, Wiener too was thinking about the limits of the human. The play introduces some discussion of artistic responses to these fields of inquiry through exploring the writings of Samuel Beckett and J. M. Coetzee. The play also addresses ethical questions about the uses of research. Both Wiener and Köhler used their work on the humanities in order to address our obligation to the human. At the same time, the play addresses our relations to those who, for reasons of ideology, “fall” outside of our definitions of the fully human. Much of the persuasive power of the work arises from the uncanny performances and in particular the staging of a life-sized wooden chimpanzee puppet. In this sense, the work makes an argument about meaning as embodied.
Para além do corpo moderno
Authors: Do Vale Lilian 
Published: January 2020

Buscando ir além da crítica às representações do corpo predominantes na Modernidade, o presente artigo recorre ao Perí psychés, texto em que Aristóteles apresenta sua teoria da unidade do corpo e da alma, para sugerir a possibilidade de superação formal dessa dualidade; mas busca, em seguida, na verve foucaultiana os termos de uma reflexão sobre as tensões que marcam as experiências do corpo em um continuum que vai da negação à presença.
Restless legs syndrome and periodic limb movements of sleep
Authors: O'Regan David , Anderson Kirstie N 
Published: January 2020

Restless legs syndrome describes a curious need to stretch and move in response to an unpleasant feeling in the legs. For most people who have this, as they are drifting off to sleep there is a twitch of the toe, foot and ankle followed by another and then another. The limb movements are strikingly periodic and are described as such by frustrated bed partners. The pathophysiology remains debated but is likely to involve dopaminergic dysfunction and ascending arousal systems. Despite a prevalence of 5–10% in the general population, the variable nature of symptoms and difficulty describing the dysaesthesia often leads to delay in treatment. This article outlines the diagnostic criteria, medication and comorbidities that modify symptoms, the impact of restless legs syndrome on health, and symptomatic management. Several licensed therapies are effective but the dopamine agonists in particular can cause challenging side effects and paradoxically worsen symptoms over time. Case histories are reviewed to guide physicians.