bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reconfigurable and programmable origami dielectric elastomer actuators with 3D shape morphing and emissive architectures
Authors: Wang Jiangxin , Li Shaohui , Gao Dace , Xiong Jiaqing , Lee Pooi See 
Published: December 2019

AbstractSoft actuators with the capability to generate programmable and reconfigurable motions without the use of complicated and rigid infrastructures are of great interest for the development of smart, interactive, and adaptive soft electronic systems. Here, we report a new strategy to achieve a transparent and reconfigurable actuator by using a dielectric elastomer actuator (DEA), which provides mechanical strains under electrical bias, integrated with origami ethyl cellulose (EC) paper that “instructs” the shape changes of the actuator. The actuator can be reconfigured and multiple mechanical motions can be programmed in the device by creating crease patterns that induce variations in the local stiffness to direct the actuations. With the versatile design and fabrication approach, a light emission device with dynamic shape changes was demonstrated.
Organic molecular crystal-based photosynaptic devices for an artificial visual-perception system
Authors: Deng Wei , Zhang Xiujuan , Jia Ruofei , Huang Liming , Zhang Xiaohong , Jie Jiansheng 
Published: December 2019

AbstractRecreating the visual-perception properties using organic electronic devices is highly desired for visual prosthetics and artificial intelligence. Although the integration of organic light-sensing components with synaptic devices can realize the recognition and memory functions for perceived images, complicated problems in device integration for practical applications are generally encountered. Here we demonstrate a new type of organic photosynaptic device based on organic molecular crystals, which can provide optical-sensing and synaptic functions together in one device by means of a unique photon-induced charge transfer effect. This device successfully emulates the working principles of human visual perception in terms of short-term plasticity, long-term potentiation, and spike-timing-dependent plasticity. Moreover, a proof-of-concept artificial image-perception system is demonstrated by integrating the photosynapses on a flexible substrate. The new devices using organic semiconductors may open up innovative application areas, such as artificially intelligent electronic and perception systems, and facilitate the integration of such devices into next-generation flexible and stretchable electronics.
Diagnostic yield of panel-based genetic testing in syndromic inherited retinal disease
Authors:   UK Inherited Retinal Disease Consortium, Jiman Omamah A. , Taylor Rachel L. , Lenassi Eva , Smith Jill Clayton , Douzgou Sofia , Ellingford Jamie M. , Barton Stephanie , Hardcastle Claire , Fletcher Tracy , Campbell Christopher , Ashworth Jane , Biswas Susmito , Ramsden Simon C. , Manson Forbes D. , Black Graeme C. 
Published: December 2019

AbstractThirty percent of all inherited retinal disease (IRD) is accounted for by conditions with extra-ocular features. This study aimed to establish the genetic diagnostic pick-up rate for IRD patients with one or more extra-ocular features undergoing panel-based screening in a clinical setting. One hundred and six participants, tested on a gene panel which contained both isolated and syndromic IRD genes, were retrospectively ascertained from the Manchester Genomic Diagnostics Laboratory database spanning 6 years (2012–2017). Phenotypic features were extracted from the clinical notes and classified according to Human Phenotype Ontology; all identified genetic variants were interpreted in accordance to the American College of Medical Genetics and Genomics guidelines. Overall, 49% (n = 52) of patients received a probable genetic diagnosis. A further 6% (n = 6) had a single disease-associated variant in an autosomal recessive disease-relevant gene. Fifty-two percent (n = 55) of patients had a clinical diagnosis at the time of testing. Of these, 71% (n = 39) received a probable genetic diagnosis. By contrast, for those without a provisional clinical diagnosis (n = 51), only 25% (n = 13) received a probable genetic diagnosis. The clinical diagnosis of Usher (n = 33) and Bardet–Biedl syndrome (n = 10) was confirmed in 67% (n = 22) and 80% (n = 8), respectively. The testing diagnostic rate in patients with clinically diagnosed multisystemic IRD conditions was significantly higher than those without one (71% versus 25%;pvalue < 0.001). The lower pick-up rate in patients without a clinical diagnosis suggests that panel-based approaches are unlikely to be the most effective means of achieving a molecular diagnosis for this group. Here, we suggest that genome-wide approaches (whole exome or genome) are more appropriate.
Molecular determinants of nephron vascular specialization in the kidney
Authors: Barry David M. , McMillan Elizabeth A. , Kunar Balvir , Lis Raphael , Zhang Tuo , Lu Tyler , Daniel Edward , Yokoyama Masataka , Gomez-Salinero Jesus M. , Sureshbabu Angara , Cleaver Ondine , Di Lorenzo Annarita , Choi Mary E. , Xiang Jenny , Redmond David , Rabbany Sina Y. , Muthukumar Thangamani , Rafii Shahin 
Published: December 2019

AbstractAlthough kidney parenchymal tissue can be generated in vitro, reconstructing the complex vasculature of the kidney remains a daunting task. The molecular pathways that specify and sustain functional, phenotypic and structural heterogeneity of the kidney vasculature are unknown. Here, we employ high-throughput bulk and single-cell RNA sequencing of the non-lymphatic endothelial cells (ECs) of the kidney to identify the molecular pathways that dictate vascular zonation from embryos to adulthood. We show that the kidney manifests vascular-specific signatures expressing defined transcription factors, ion channels, solute transporters, and angiocrine factors choreographing kidney functions. Notably, the ontology of the glomerulus coincides with induction of unique transcription factors, including Tbx3, Gata5, Prdm1, and Pbx1. Deletion of Tbx3 in ECs results in glomerular hypoplasia, microaneurysms and regressed fenestrations leading to fibrosis in subsets of glomeruli. Deciphering the molecular determinants of kidney vascular signatures lays the foundation for rebuilding nephrons and uncovering the pathogenesis of kidney disorders.
Structural basis of denuded glycan recognition by SPOR domains in bacterial cell division
Authors: Alcorlo Martín , Dik David A. , De Benedetti Stefania , Mahasenan Kiran V. , Lee Mijoon , Domínguez-Gil Teresa , Hesek Dusan , Lastochkin Elena , López Daniel , Boggess Bill , Mobashery Shahriar , Hermoso Juan A. 
Published: December 2019

AbstractSPOR domains are widely present in bacterial proteins that recognize cell-wall peptidoglycan strands stripped of the peptide stems. This type of peptidoglycan is enriched in the septal ring as a product of catalysis by cell-wall amidases that participate in the separation of daughter cells during cell division. Here, we document binding of synthetic denuded glycan ligands to the SPOR domain of the lytic transglycosylase RlpA from Pseudomonas aeruginosa (SPOR-RlpA) by mass spectrometry and structural analyses, and demonstrate that indeed the presence of peptide stems in the peptidoglycan abrogates binding. The crystal structures of the SPOR domain, in the apo state and in complex with different synthetic glycan ligands, provide insights into the molecular basis for recognition and delineate a conserved pattern in other SPOR domains. The biological and structural observations presented here are followed up by molecular-dynamics simulations and by exploration of the effect on binding of distinct peptidoglycan modifications.
Intense attosecond pulses carrying orbital angular momentum using laser plasma interactions
Authors: Wang J. W. , Zepf M. , Rykovanov S. G. 
Published: December 2019

AbstractLight beams with helical phase-fronts are known to carry orbital angular momentum (OAM) and provide an additional degree of freedom to beams of coherent light. While OAM beams can be readily derived from Gaussian laser beams with phase plates or gratings, this is far more challenging in the extreme ultra-violet (XUV), especially for the case of high XUV intensity. Here, we theoretically and numerically demonstrate that intense surface harmonics carrying OAM are naturally produced by the intrinsic dynamics of a relativistically intense circularly-polarized Gaussian beam (i.e. non-vortex) interacting with a target at normal incidence. Relativistic surface oscillations convert the laser pulses to intense XUV harmonic radiation via the well-known relativistic oscillating mirror mechanism. We show that the azimuthal and radial dependence of the harmonic generation process converts the spin angular momentum of the laser beam to orbital angular momentum resulting in an intense attosecond pulse (or pulse train) with OAM.
Functional significance of U2AF1 S34F mutations in lung adenocarcinomas
Authors: Esfahani Mohammad S. , Lee Luke J. , Jeon Young-Jun , Flynn Ryan A. , Stehr Henning , Hui Angela B. , Ishisoko Noriko , Kildebeck Eric , Newman Aaron M. , Bratman Scott V. , Porteus Matthew H. , Chang Howard Y. , Alizadeh Ash A. , Diehn Maximilian 
Published: December 2019

AbstractThe functional role of U2AF1 mutations in lung adenocarcinomas (LUADs) remains incompletely understood. Here, we report a significant co-occurrence of U2AF1 S34F mutations with ROS1 translocations in LUADs. To characterize this interaction, we profiled effects of S34F on the transcriptome-wide distribution of RNA binding and alternative splicing in cells harboring the ROS1 translocation. Compared to its wild-type counterpart, U2AF1 S34F preferentially binds and modulates splicing of introns containing CAG trinucleotides at their 3′ splice junctions. The presence of S34F caused a shift in cross-linking at 3′ splice sites, which was significantly associated with alternative splicing of skipped exons. U2AF1 S34F induced expression of genes involved in the epithelial-mesenchymal transition (EMT) and increased tumor cell invasion. Finally, S34F increased splicing of the long over the short SLC34A2-ROS1 isoform, which was also associated with enhanced invasiveness. Taken together, our results suggest a mechanistic interaction between mutant U2AF1 and ROS1 in LUAD.
Sub-cycle time resolution of multi-photon momentum transfer in strong-field ionization
Authors: Willenberg Benjamin , Maurer Jochen , Mayer Benedikt W. , Keller Ursula 
Published: December 2019

AbstractDuring multi-photon ionization of an atom it is well understood how the involved photons transfer their energy to the ion and the photoelectron. However, the transfer of the photon linear momentum is still not fully understood. Here, we present a time-resolved measurement of linear momentum transfer along the laser pulse propagation direction. We can show that the linear momentum transfer to the photoelectron depends on the ionization time within the laser cycle using the attoclock technique. We can mostly explain the measured linear momentum transfer within a classical model for a free electron in a laser field. However, corrections are required due to the parent-ion interaction and due to the initial momentum when the electron enters the continuum. The parent-ion interaction induces a negative attosecond time delay between the appearance in the continuum of the electron with minimal linear momentum transfer and the point in time with maximum ionization rate.
Heterogeneity and dynamics of active Kras-induced dysplastic lineages from mouse corpus stomach
Authors: Min Jimin , Vega Paige N. , Engevik Amy C. , Williams Janice A. , Yang Qing , Patterson Loraine M. , Simmons Alan J. , Bliton R. Jarrett , Betts Joshua W. , Lau Ken S. , Magness Scott T. , Goldenring James R. , Choi Eunyoung 
Published: December 2019

AbstractDysplasia is considered a key transition state between pre-cancer and cancer in gastric carcinogenesis. However, the cellular or phenotypic heterogeneity and mechanisms of dysplasia progression have not been elucidated. We have established metaplastic and dysplastic organoid lines, derived from Mist1-Kras(G12D) mouse stomach corpus and studied distinct cellular behaviors and characteristics of metaplastic and dysplastic organoids. We also examined functional roles for Kras activation in dysplasia progression using Selumetinib, a MEK inhibitor, which is a downstream mediator of Kras signaling. Here, we report that dysplastic organoids die or show altered cellular behaviors and diminished aggressive behavior in response to MEK inhibition. However, the organoids surviving after MEK inhibition maintain cellular heterogeneity. Two dysplastic stem cell (DSC) populations are also identified in dysplastic cells, which exhibited different clonogenic potentials. Therefore, Kras activation controls cellular dynamics and progression to dysplasia, and DSCs might contribute to cellular heterogeneity in dysplastic cell lineages.
An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function
Authors: Xu Qiang , Tang Chunlei , Wang Xiaodong , Sun Shutian , Zhao Jinren , Kang Zhensheng , Wang Xiaojie 
Published: December 2019

AbstractChloroplasts are important for photosynthesis and for plant immunity against microbial pathogens. Here we identify a haustorium-specific protein (Pst_12806) from the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), that is translocated into chloroplasts and affects chloroplast function. Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants and reduces Pseudomonas-induced hypersensitive response in wheat. It suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes. Pst_12806 is upregulated during infection, and its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation. Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein (a putative component of the cytochrome b6-f complex). Expression of Pst_12806 in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS. Silencing TaISP by virus-induced gene silencing in a susceptible wheat cultivar reduces fungal growth and uredinium development, suggesting an increase in resistance against Pst infection.
Compact and ultra-efficient broadband plasmonic terahertz field detector
Authors: Salamin Yannick , Benea-Chelmus Ileana-Cristina , Fedoryshyn Yuriy , Heni Wolfgang , Elder Delwin L. , Dalton Larry R. , Faist Jérôme , Leuthold Juerg 
Published: December 2019

AbstractTerahertz sources and detectors have enabled numerous new applications from medical to communications. Yet, most efficient terahertz detection schemes rely on complex free-space optics and typically require high-power lasers as local oscillators. Here, we demonstrate a fiber-coupled, monolithic plasmonic terahertz field detector on a silicon-photonics platform featuring a detection bandwidth of 2.5 THz with a 65 dB dynamical range. The terahertz wave is measured through its nonlinear mixing with an optical probe pulse with an average power of only 63 nW. The high efficiency of the scheme relies on the extreme confinement of the terahertz field to a small volume of 10−8(λTHz/2)3. Additionally, on-chip guided plasmonic probe beams sample the terahertz signal efficiently in this volume. The approach results in an extremely short interaction length of only 5 μm, which eliminates the need for phase matching and shows the highest conversion efficiency per unit length up to date.
Resonant optical Stark effect in monolayer WS2
Authors: Cunningham Paul D. , Hanbicki Aubrey T. , Reinecke Thomas L. , McCreary Kathleen M. , Jonker Berend T. 
Published: December 2019

AbstractBreaking the valley degeneracy in monolayer transition metal dichalcogenides through the valley-selective optical Stark effect (OSE) can be exploited for classical and quantum valleytronic operations such as coherent manipulation of valley superposition states. The strong light-matter interactions responsible for the OSE have historically been described by a two-level dressed-atom model, which assumes noninteracting particles. Here we experimentally show that this model, which works well in semiconductors far from resonance, does not apply for excitation near the exciton resonance in monolayer WS2. Instead, we show that an excitonic model of the OSE, which includes many-body Coulomb interactions, is required. We confirm the prediction from this theory that many-body effects between virtual excitons produce a dominant blue-shift for photoexcitation detuned from resonance by less than the exciton binding energy. As such, we suggest that our findings are general to low-dimensional semiconductors that support bound excitons and other many-body Coulomb interactions.
Superhydrophobic hemostatic nanofiber composites for fast clotting and minimal adhesion
Authors: Li Zhe , Milionis Athanasios , Zheng Yu , Yee Marcus , Codispoti Lukas , Tan Freddie , Poulikakos Dimos , Yap Choon Hwai 
Published: December 2019

AbstractHemostatic materials are of great importance in medicine. However, their successful implementation is still challenging as it depends on two, often counteracting, attributes; achieving blood coagulation rapidly, before significant blood loss, and enabling subsequent facile wound-dressing removal, without clot tears and secondary bleeding. Here we illustrate an approach for achieving hemostasis, rationally targeting both attributes, via a superhydrophobic surface with immobilized carbon nanofibers (CNFs). We find that CNFs promote quick fibrin growth and cause rapid clotting, and due to their superhydrophobic nature they severely limit blood wetting to prevent blood loss and drastically reduce bacteria attachment. Furthermore, minimal contact between the clot and the superhydrophobic CNF surface yields an unforced clot detachment after clot shrinkage. All these important attributes are verified in vitro and in vivo with rat experiments. Our work thereby demonstrates that this strategy for designing hemostatic patch materials has great potential.
Controlling energy levels and Fermi level en route to fully tailored energetics in organic semiconductors
Authors: Warren Ross , Privitera Alberto , Kaienburg Pascal , Lauritzen Andreas E. , Thimm Oliver , Nelson Jenny , Riede Moritz K. 
Published: December 2019

AbstractSimultaneous control over both the energy levels and Fermi level, a key breakthrough for inorganic electronics, has yet to be shown for organic semiconductors. Here, energy level tuning and molecular doping are combined to demonstrate controlled shifts in ionisation potential and Fermi level of an organic thin film. This is achieved by p-doping a blend of two host molecules, zinc phthalocyanine and its eight-times fluorinated derivative, with tunable energy levels based on mixing ratio. The doping efficiency is found to depend on host mixing ratio, which is explained using a statistical model that includes both shifts of the host’s ionisation potentials and, importantly, the electron affinity of the dopant. Therefore, the energy level tuning effect has a crucial impact on the molecular doping process. The practice of comparing host and dopant energy levels must consider the long-range electrostatic shifts to consistently explain the doping mechanism in organic semiconductors.
All-fiber tribo-ferroelectric synergistic electronics with high thermal-moisture stability and comfortability
Authors: Yang Weifeng , Gong Wei , Hou Chengyi , Su Yun , Guo Yinben , Zhang Wei , Li Yaogang , Zhang Qinghong , Wang Hongzhi 
Published: December 2019

AbstractDeveloping fabric-based electronics with good wearability is undoubtedly an urgent demand for wearable technologies. Although the state-of-the-art fabric-based wearable devices have shown unique advantages in the field of e-textiles, further efforts should be made before achieving “electronic clothing” due to the hard challenge of optimally unifying both promising electrical performance and comfortability in single device. Here, we report an all-fiber tribo-ferroelectric synergistic e-textile with outstanding thermal-moisture comfortability. Owing to a tribo-ferroelectric synergistic effect introduced by ferroelectric polymer nanofibers, the maximum peak power density of the e-textile reaches 5.2 W m−2 under low frequency motion, which is 7 times that of the state-of-the-art breathable triboelectric textiles. Electronic nanofiber materials form hierarchical networks in the e-textile hence lead to moisture wicking, which contributes to outstanding thermal-moisture comfortability of the e-textile. The all-fiber electronics is reliable in complicated real-life situation. Therefore, it is an idea prototypical example for electronic clothing.
The SIGMA rat brain templates and atlases for multimodal MRI data analysis and visualization
Authors: Barrière D. A. , Magalhães R. , Novais A. , Marques P. , Selingue E. , Geffroy F. , Marques F. , Cerqueira J. , Sousa J. C. , Boumezbeur F. , Bottlaender M. , Jay T. M. , Cachia A. , Sousa N. , Mériaux S. 
Published: December 2019

AbstractPreclinical imaging studies offer a unique access to the rat brain, allowing investigations that go beyond what is possible in human studies. Unfortunately, these techniques still suffer from a lack of dedicated and standardized neuroimaging tools, namely brain templates and descriptive atlases. Here, we present two rat brain MRI templates and their associated gray matter, white matter and cerebrospinal fluid probability maps, generated from ex vivo $${\mathrm{T}}_2^ \ast$$

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-weighted images (90 µm isotropic resolution) and in vivo T2-weighted images (150 µm isotropic resolution). In association with these templates, we also provide both anatomical and functional 3D brain atlases, respectively derived from the merging of the Waxholm and Tohoku atlases, and analysis of resting-state functional MRI data. Finally, we propose a complete set of preclinical MRI reference resources, compatible with common neuroimaging software, for the investigation of rat brain structures and functions.
Transcription-mediated organization of the replication initiation program across large genes sets common fragile sites genome-wide
Authors: Brison Olivier , El-Hilali Sami , Azar Dana , Koundrioukoff Stéphane , Schmidt Mélanie , Nähse Viola , Jaszczyszyn Yan , Lachages Anne-Marie , Dutrillaux Bernard , Thermes Claude , Debatisse Michelle , Chen Chun-Long 
Published: December 2019

AbstractCommon fragile sites (CFSs) are chromosome regions prone to breakage upon replication stress known to drive chromosome rearrangements during oncogenesis. Most CFSs nest in large expressed genes, suggesting that transcription could elicit their instability; however, the underlying mechanisms remain elusive. Genome-wide replication timing analyses here show that stress-induced delayed/under-replication is the hallmark of CFSs. Extensive genome-wide analyses of nascent transcripts, replication origin positioning and fork directionality reveal that 80% of CFSs nest in large transcribed domains poor in initiation events, replicated by long-travelling forks. Forks that travel long in late S phase explains CFS replication features, whereas formation of sequence-dependent fork barriers or head-on transcription–replication conflicts do not. We further show that transcription inhibition during S phase, which suppresses transcription–replication encounters and prevents origin resetting, could not rescue CFS stability. Altogether, our results show that transcription-dependent suppression of initiation events delays replication of large gene bodies, committing them to instability.