bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sirt6 opposes glycochenodeoxycholate-induced apoptosis of biliary epithelial cells through the AMPK/PGC-1α pathway
Authors: Li Jiye , Yu Dongsheng , Chen Sanyang , Liu Yifan , Shi Jihua , Zhang Jiakai , Wen Peihao , Wang Zhihui , Li Jie , Guo Wenzhi , Zhang Shuijun 
Published: April 2020

Abstract
Background
Induction of biliary epithelial cell apoptosis by toxic bile acids is involved in the development of cholestatic disease, but the underlying molecular mechanism is not clear. The purpose of this study was to investigate the molecular mechanisms involved in Sirt6 protection against the apoptosis of human intrahepatic biliary epithelial cells (HiBEC) induced by the bile acid glycochenodeoxycholate (GCDC).

Results
Sirt6 was either overexpressed or knocked down in HiBEC, with or without GCDC pretreatment. The CCK-8 assay was used to assess cell viability and, Hoechst 33258 staining was used to determine apoptotic rate. Mitochondrial DNA (mtDNA) copy number, malondialdehyde (MDA) and reactive oxygen species (ROS) production were detected to evaluate the severity of the mitochondrial dysfunction and oxidative stress. The mRNA and protein levels of PGC-1α, Nrf1, and Nrf2 were analyzed using RT-qPCR and western blot assay. The results showed that Sirt6 opposed GCDC-induced apoptosis in HiBEC via up-regulating PGC-1α expression and stabilizing mtDNA. We used agonists and inhibitors of AMPK to demonstrate that Sirt6 increased PGC-1α expression through the AMPK pathway whereas GCDC had the opposite effect. Finally, western blot, luciferase assay, and co-immunoprecipitation were used to describe a direct interaction and acetylation modification of PGC-1α by Sirt6.

Conclusion
Our data illuminated that Sirt6 ameliorated GCDC-induced HiBEC apoptosis by upregulating PGC-1α expression through the AMPK pathway and its deacetylation effect.
Hepatitis E virus seroprevalence in pets in the Netherlands and the permissiveness of canine liver cells to the infection
Authors: Li Yunlong , Qu Changbo , Spee Bart , Zhang Ruyi , Penning Louis C. , de Man Robert A. , Peppelenbosch Maikel P. , Fieten Hille , Pan Qiuwei 
Published: April 2020

AbstractHepatitis E virus (HEV) as an emerging zoonotic pathogen causes a major public health issue. Transmission from domestic, wildlife and zoo animals to human has been widely reported. Whether pets also serve as reservoirs remains an intriguing question. In this study, we found the sero-positive rates of HEV-specific antibodies in pet dogs, cats and horses of 18.52% (30/162), 14.89% (7/47) and 18.18% (4/22) in the Netherlands. Although HEV viral RNA was not detected in these animals, we have demonstrated that dog liver cells are susceptible to HEV infection in vitro. These results call more attention to address the potential role of pets in the zoonotic transmission of HEV.
Structure and function of the Arctic and Antarctic marine microbiota as revealed by metagenomics
Authors: Cao Shunan , Zhang Weipeng , Ding Wei , Wang Meng , Fan Shen , Yang Bo , Mcminn Andrew , Wang Min , Xie Bin-bin , Qin Qi-Long , Chen Xiu-Lan , He Jianfeng , Zhang Yu-Zhong 
Published: April 2020

AbstractBackgroundThe Arctic and Antarctic are the two most geographically distant bioregions on earth. Recent sampling efforts and following metagenomics have shed light on the global ocean microbial diversity and function, yet the microbiota of polar regions has not been included in such global analyses.ResultsHere a metagenomic study of seawater samples (n= 60) collected from different depths at 28 locations in the Arctic and Antarctic zones was performed, together with metagenomes from theTaraOceans. More than 7500 (19%) polar seawater-derived operational taxonomic units could not be identified in theTaraOceans datasets, and more than 3,900,000 protein-coding gene orthologs had no hits in the Ocean Microbial Reference Gene Catalog. Analysis of 214 metagenome assembled genomes (MAGs) recovered from the polar seawater microbiomes, revealed strains that are prevalent in the polar regions while nearly undetectable in temperate seawater. Metabolic pathway reconstruction for these microbes suggested versatility for saccharide and lipids biosynthesis, nitrate and sulfate reduction, and CO2fixation. Comparison between the Arctic and Antarctic microbiomes revealed that antibiotic resistance genes were enriched in the Arctic while functions like DNA recombination were enriched in the Antarctic.ConclusionsOur data highlight the occurrence of dominant and locally enriched microbes in the Arctic and Antarctic seawater with unique functional traits for environmental adaption, and provide a foundation for analyzing the global ocean microbiome in a more complete perspective.
Nutrition and physical activity intervention for families with familial hypercholesterolaemia: protocol for a pilot randomised controlled feasibility study
Authors: Kinnear Fiona J. , Hamilton-Shield Julian P. , Stensel David J. , Bayly Graham , Searle Aidan , Thackray Alice E. , Lithander Fiona E. 
Published: April 2020

AbstractBackgroundUntreated heterozygous familial hypercholesterolaemia (FH) causes high low-density lipoprotein cholesterol (LDL-C) levels and increased cardiovascular disease (CVD) risk. Despite pharmacological treatment, many treated individuals remain at higher CVD risk than non-affected individuals. This may be due to LDL-C targets not being met and presence of other CVD risk factors. Adhering to dietary and physical activity (PA) recommendations developed for individuals with FH may further reduce CVD risk. However, there is insufficient research to support the efficacy of adhering to these guidelines on LDL-C and other CVD risk factors. The need for studies to investigate the effectiveness of nutrition and PA interventions in the FH population has been widely recognised and recommended. This paper describes the protocol of a pilot, randomised controlled trial designed to evaluate the feasibility and acceptability of a specifically developed nutrition and PA intervention aimed at improving the dietary intakes and PA levels of families with FH.MethodsA two-arm randomised waitlist-controlled pilot trial will be conducted across three National Health Service (NHS) sites in England, UK. Twenty-four young people with FH, aged 10–18 years, and their affected parent, will be recruited and randomly assigned to the intervention or waitlist and usual care control. The primary aim is to provide evidence for the feasibility and acceptability of delivering the intervention, explored quantitatively (rates of recruitment, retention and outcome measure completeness) and qualitatively (qualitative interviews). The secondary aim is to provide evidence for the potential efficacy of the intervention on dietary intake, PA, sedentary time, body composition, CVD risk factors and quality of life determined at baseline and endpoint assessments. The intervention will involve an hour-long consultation with a dietitian at baseline and four follow-up contacts across the 12-week intervention. It has been specifically developed for use with individuals with FH and incorporates behavioural change techniques to target identified enablers and barriers to adherence in this population.DiscussionThis trial will estimate the feasibility and acceptability of the nutrition and PA intervention delivered to young people and parents with FH. If appropriate, this study can be used to inform the design of an adequately powered definitive trial.Trial registrationISRCTN, ISRCTN24880714. Registered 07/06/2018,http://www.isrctn.com/ISRCTN24880714.
Long-term impact of the expansion of a hospital liaison psychiatry service on patient care and costs following emergency department attendances for self-harm
Authors: Jackson Joni , Nugawela Manjula D. , De Vocht Frank , Moran Paul , Hollingworth William , Knipe Duleeka , Munien Nik , Gunnell David , Redaniel Maria Theresa 
Published: April 2020

Background
In September 2014, as part of a national initiative to increase access to liaison psychiatry services, the liaison psychiatry services at Bristol Royal Infirmary received new investment of £250 000 per annum, expanding its availability from 40 to 98 h per week. The long-term impact on patient outcomes and costs, of patients presenting to the emergency department with self-harm, is unknown.

Aims
To assess the long-term impact of the investment on patient care outcomes and costs, of patients presenting to the emergency department with self-harm.

Method
Monthly data for all self-harm emergency department attendances between 1 September 2011 and 30 September 2017 was modelled using Bayesian structural time series to estimate expected outcomes in the absence of expanded operating hours (the counterfactual). The difference between the observed and expected trends for each outcome were interpreted as the effects of the investment.

Results
Over the 3 years after service expansion, the mean number of self-harm attendances increased 13%. Median waiting time from arrival to psychosocial assessment was 2 h shorter (18.6% decrease, 95% Bayesian credible interval (BCI) −30.2% to −2.8%), there were 45 more referrals to other agencies (86.1% increase, 95% BCI 60.6% to 110.9%) and a small increase in the number of psychosocial assessments (11.7% increase, 95% BCI −3.4% to 28.5%) per month. Monthly mean net hospital costs were £34 more per episode (5.3% increase, 95% BCI −11.6% to 25.5%).

Conclusions
Despite annual increases in emergency department attendances, investment was associated with reduced waiting times for psychosocial assessment and more referrals to other agencies, with only a small increase in cost per episode.
Barriers to Participation in Therapeutic Clinical Trials as Perceived by Community Oncologists
Authors: Wong Andrew R. , Sun Virginia , George Kevin , Liu Jennifer , Padam Simran , Chen Brandon A. , George Thomas , Amini Arya , Li Daneng , Sedrak Mina S. 
Published: April 2020

PURPOSE: Despite considerable research on the barriers to enrollment in cancer therapeutic trials, few studies have elicited barriers from the perspective of community physicians, who provide the majority of cancer care. The purpose of this study was to characterize barriers to and facilitators of cancer therapeutic trials as perceived by oncologists in community practices. METHODS: Twenty semistructured interviews were conducted with oncologists at six community sites affiliated with City of Hope National Medical Center from March to June 2018. Responses were recorded digitally and transcribed. Data were analyzed using qualitative content analysis. RESULTS: Of the 20 participants, 4 (20%) were women, 13 (65%) had > 10 years of practice experience, and 16 (80%) reported that < 5% of their patients were enrolled in a therapeutic trial. Participants identified four system-level barriers: lack of appropriate trials for community-based settings, insufficient infrastructure support, restrictive eligibility criteria, and financial limitations; three physician-level barriers: lack of awareness of available trials, lack of knowledge of trial details, and lack of time; and two patient-level barriers: patient burden and negative beliefs/attitudes toward research. Efforts aimed to increase trial availability, clinical trial support personnel, and physician knowledge were identified as major facilitators. CONCLUSION: Community oncologists face numerous complex, multifaceted barriers to cancer therapeutic trial enrollment. Although expanding clinical research beyond the academic setting allows access to a larger and more diverse patient population, increasing generalizability and relevance of trial findings, there remains a substantial need for new strategies to improve cancer research delivery in the community.
Quality of reporting acupuncture interventions for chronic obstructive pulmonary disease: Review of adherence to the STRICTA statement
Authors: Fernández-Jané Carles , Solà-Madurell Mireia , Yu Mingkun , Liang Changhao , Fei Yutong , Sitjà-Rabert Mercè , Úrrutia Gerard 
Published: April 2020

Background:The quality of reporting of acupuncture interventions is critical to ensure the applicability and reproducibility of acupuncture clinical trials. In the past, different publications have evaluated the quality of reporting of acupuncture interventions for different clinical situations, such as knee osteoarthritis, neurological diseases or cancer. However, this has not been done for acupuncture trials for chronic obstructive pulmonary disease (COPD).Objective:To assess the quality of reporting of acupuncture interventions in trials for COPD.Methods:A total of 11 English and Chinese databases were screened up until May 2019 for randomised or quasi-randomised control trials of acupuncture for COPD. The STRICTA checklist was used to determine the quality of the reporting of acupuncture interventions.Results:A total of 28 trials were included in our review. Out of the 16 STRICTA checklist subitems analysed, only 3 were considered appropriately reported in more than 70% of the trials, while 7 were correctly reported in less than 40%.Conclusion:The adherence to STRICTA guidelines of acupuncture trials for COPD is suboptimal, and future efforts need to be addressed to improve the quality of reporting.
Acceptability and feasibility of genital self-sampling for the diagnosis of female genital schistosomiasis: a cross-sectional study in Zambia
Authors: Rutty Phiri Comfort , Sturt Amy S. , Webb Emily L. , Chola Namakau , Hayes Richard , Shanaube Kwame , Ayles Helen , Hansingo Isaiah , Bustinduy Amaya L. ,   BILHIV study team
Published: April 2020

Background: Female genital schistosomiasis (FGS) is a neglected and disabling gynaecological disorder that is difficult to diagnose and is part of the wider spectrum of urogenital disease caused by the waterborne parasite Schistosoma haematobium. Over 90% of human schistosomiasis cases are found in sub-Saharan Africa with 3.8 million people infected with schistosomes in Zambia. Reported FGS prevalence ranges from 33-75% of those with urinary schistosomiasis in endemic areas, suggesting a potentially high FGS burden in Zambia alone. The Bilharzia and HIV (BILHIV) study evaluated home self-sampling genital collection methods for the diagnosis of FGS. Methods: Eligible participants included non-pregnant, sexually active women aged 18-31 who were previously recruited for the HPTN 071 (PopART) trial in Livingstone, Zambia. Household demographic and symptom questionnaires were administered by community workers. Participants were offered vaginal and cervical self-swabs and a urine cup. Cervicovaginal lavage (CVL) was performed in clinic by midwives. Information was collected from participants on the acceptability and feasibility of genital self-sampling. Results: From January-August 2018, 603 women were enrolled, and 87.3% (527/603) completed clinic follow up. A high proportion of participants indicated that self-collection of specimens was “easy” or “very easy” on a 5-point Likert scale. A high proportion of women would be willing to self-collect all three specimens again in future: vaginal swab 96.7% (583/603), cervical swab 96.5% (582/603), and urine 96.2% (580/603). Home-based self-sampling was preferred over provider-based sampling in the clinic due to greater privacy 58.5% (353/603), convenience 46.3% (279/603) and need for transportation 15.9% (96/603). Conclusions: Home based genital self-sampling for FGS diagnosis is highly acceptable. This scalable method may inform future efforts for community-based diagnosis of FGS.
Formation of Functional Literacy of Primary School Children in the Field of Healthy and Safe Lifestyle
Authors: Artyuhova Irina 
Published: April 2020

The article discusses the basic principles and specifics of the organization of activities of primary school teachers in the formation of students’
knowledge in the field of safe behavior and healthy lifestyle, as well as their development of practical skills in relevant situations. Questions of
didactic support are highlighted, target methodological modules are marked. As an example of their use, the development of classes on the topic “My safety when riding a scooter and crossing the roadway” is presented.
Current concepts and outcomes in cemented femoral stem design and cementation techniques: the argument for a new classification system
Authors: Cassar-Gheiti Adrian J. , McColgan Rosie , Kelly Martin , Cassar-Gheiti Theresa M. , Kenny Paddy , Murphy Colin G. 
Published: April 2020

Cemented implant fixation design principles have evolved since the 1950s, and various femoral stem designs are currently in use to provide a stable construct between the implant–cement and cement–bone interfaces. Cemented stems have classically been classified into two broad categories: taper slip or force closed, and composite beams or shaped closed designs. While these simplifications are acceptable general categories, there are other important surgical details that need to be taken into consideration such as different broaching techniques, cementing techniques and mantle thickness. With the evolution of cemented implants, the introduction of newer implants which have hybrid properties, and the use of different broaching techniques, the classification of a very heterogenous group of implants into simple binary categories becomes increasingly difficult. A more comprehensive classification system would aid in comparison of results and better understanding of the implants’ biomechanics. We review these differing stem designs, their respective cementing techniques and geometries. We then propose a simple four-part classification system and summarize the long-term outcomes and international registry data for each respective type of cemented prosthesis.Cite this article: EFORT Open Rev 2020;5:241-252. DOI: 10.1302/2058-5241.5.190034
Graft choice in combined anterior cruciate ligament and medial collateral ligament reconstruction
Authors: Figueroa Francisco , Figueroa David , Calvo Rafael , Vaisman Alex , Espregueira-Mendes João 
Published: April 2020

There is a concern regarding which grafts should be used in combined anterior cruciate ligament (ACL) and medial collateral ligament (MCL) reconstructions, with a paucity of recommendations focused on this specific topic. Expert opinions suggest the use of allograft-only reconstructions to limit donor-site morbidity or using at least one allograft and one autograft. When a hamstring tendon autograft is harvested, techniques that maintain both the integrity of the sartorius fascia and the gracilis are recommended because of the role that the ST-G-S (semitendinosus-gracilis-sartorius) complex plays in valgus stability in the setting of an MCL-deficient knee. Cite this article: EFORT Open Rev 2020;5:221-225. DOI: 10.1302/2058-5241.5.190049
Optimized Gaussian model for non-uniform heating compensation in pulsed thermography
Authors: Erazo-Aux Jorge , Loaiza-Correa H. , Restrepo-Giron A. D. , Alfonso-Morales W. 
Published: April 2020

This paper presents a new, to the best of our knowledge, methodology for the thermal compensation of background heating in thermograms of composites. The technique analyzes the spatial data of the thermal images obtained from a pulsed thermography inspection and automatically calculates the optimal parameters of a predefined objective function. These parameters are obtained by curve fitting using the least squares method and model the temperature distribution of the image background using the proposed objective function. To verify the methodology, we use real and synthetic images of a sample of carbon-fiber-reinforced plastic (CFRP) with defects, with diameter/depth ratios that range between 15.0 and 75.0 and between 1.7 and 90.0, respectively. The performance of the method is tested using a local and a global definition of the signal-to-noise ratio (SNR) and is statistically validated by analysis of variance. The average performance values obtained were

55.0

dB

and

7.0

dB

on synthetic images and real images, respectively. The proposed method provides superior and statistically significant differences compared to techniques reported in the literature for contrast enhancement [e.g., differential absolute contrast (DAC) and background thermal compensation by filtering (BTCF)]. Unlike contrast normalization (CN), the proposed technique stands out since it does not need to predefine variables, select reference regions, have prior knowledge of the partial (or complete) state of the material, or analyze totally (or partially) the temporal evolution of the temperature or any characteristic derived from it.
Bibliotecários nas redes sociais: grupo de biblioteconomia na plataforma Facebook
Authors: Senabio Kelly Pinheiro Conceição , Maia Maria de Fátima dos Santos 
Published: April 2020

Bibliotecários,são profissionais que atuam como agentes condutores da informação. Utilizar ferramentas que potencializem a construção de vínculos e conexões é um fator essencial, por isso as redes sociais são importantes para que interajam, troquem ideias, conheçam outros profissionais, esclareçam dúvidas e compartilhem informações sobre serviços e/ou pesquisas na área. Sendo assim, as redes sociais como a plataforma Facebook podem auxiliar nas atividades desenvolvidas pelos mesmos. Este trabalho tem como objetivo comparar dois grupos de biblioteconomia: “Bibliotecários do Brasil” e “Mural Interativo do Bibliotecário” tipificando a natureza das postagens publicadas. A metodologia empregada foi de natureza quali-quantitativa. Utilizou-se da coleta de dadospor meio de tabelas estruturadas aplicadas para obtenção dos resultados e análise dos principais temas discutidos nos grupos. Como resultado foi possível observar que a interação entre os profissionais da área existe e contribui para o desenvolvimento da Biblioteconomia no Brasil. Os temas mais recorrentes foram eventos, capacitação e literatura. Conclui-se que as redes sociais são um campo muito vasto para o estudo das interações dos profissionais na área biblioteconômica.
Referências em livros e artigos de periódicos: uma comparação de estilos
Authors: Sampaio Julio César Lima , Mendonça Magnaura , E Silva Marilene de Sena , Carvalho Talia Raquel Oliveira 
Published: April 2020

Referenciar é identificar individualmente um documento com base em orientações de estilos que orientam entre outras coisas a citação e as referências. O objetivo do artigo foi identificar os principais estilos, suas características na descrição de referências como a formatação da autoria pessoa física; uso do ““et al””; destaque tipográfico a abreviação de títulos de periódicos. Foram analisados oito estilos que em sua maioria possuem custo para acesso ao texto completo. O sistema autor-data é comum; os estilos variam muito em relação ao uso do ““et al””; a caixa alta foi observada na ISO 690 e NBR 6023; os estilos APA, CSE, MLA, Chicago, Harvard e Vancouver são simples; favorecem o conteúdo e são de custo acessível.
Global existence and blow-up of weak solutions for a class of fractional p-Laplacian evolution equations
Authors: Liao Menglan , Liu Qiang , Ye Hailong 
Published: April 2020

Abstract
In this paper, we study the fractional p-Laplacian evolution equation with arbitrary initial energy,

$$\begin{array}{}
\displaystyle u_t(x,t) + (-{\it\Delta})_p^s u(x,t) = f(u(x,t)), \quad x\in {\it\Omega}, \,t \gt 0,
\end{array} $$
where
$\begin{array}{}
(-{\it\Delta})_p^s
\end{array} $ is the fractional p-Laplacian with
$\begin{array}{}
p \gt \max\{\frac{2N}{N+2s},1\}
\end{array} $ and s ∈ (0, 1). Specifically, by the modified potential well method, we obtain the global existence, uniqueness, and blow-up in finite time of the weak solution for the low, critical and high initial energy cases respectively.