bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purification of Live Stem‐Cell‐Derived Islet Lineage Intermediates
Authors: Rasouli Niloofar , Melton Douglas A. , Alvarez‐Dominguez Juan R. 
Published: June 2020

AbstractStem‐cell‐derived tissues offer platforms to study organ development, model physiology during health and disease, and test novel therapies. We describe methods to isolate cells at successive stages during in vitro differentiation of human stem cells into the pancreatic endocrine lineage. Using flow cytometry, we purify live lineage intermediates in numbers not available by fetal biopsy. These include pancreatic and endocrine progenitors, isolated based on known surface markers. We further report a strategy that leverages intracellular zinc content and DPP4/CD26 expression to separate monohormonal insulin+ β cells from polyhormonal counterparts. These methods enable comprehensive molecular profiling during human islet lineage progression. © 2020 Wiley Periodicals LLC.Basic Protocol: In vitro isolation of human islet developmental intermediates
Eco‐control for corporate sustainable management: A sustainability development stage perspective
Authors: Gunarathne A. D. Nuwan , Lee Ki‐Hoon 
Published: June 2020

AbstractEco‐control offers an integrated management approach to transitioning toward corporate sustainable development, such as sustainable agriculture management. Despite the need to consider organizational and managerial changes and development in the field of sustainable agriculture, the use of integrated management controls has not yet been explored. This study identifies how the eco‐control procedures in sustainable agriculture management change when a commercial tea company transits to different stages of corporate sustainable development. It has developed an analytical framework, combining the sustainability management development perspective with the processual view of integrated eco‐control. Using a longitudinal case study approach, the data have been collected via in‐depth interviews, site visits, and document analyses, in respect of a commercial tea company in Sri Lanka. The findings show how the processual eco‐controls have changed as a result of internal and external challenges faced in the different sustainability management development stages of the transition to sustainable agriculture management. The findings emphasize the need for gaining a better understanding of the actions and decisions at the field (operational) and organizational level in promoting sustainable agriculture management practices in agribusiness firms.
Using citizen science data to inform the relative sensitivity of waterbirds to natural versus human‐dominated landscapes in China
Authors: Duan Houlang , Xia Shaoxia , Yu Xiubo , Liu Yu , Teng Jiakun , Dou Yuehan 
Published: June 2020

AbstractHabitat loss is widely regarded as one of the most destructive factors threatening native biodiversity. Because migratory waterbirds include some of the most globally endangered species, information on their sensitivity to landscape would benefit their conservation. While citizen science data on waterbird species occurrence are subjected to various biases, their appropriate interpretation can provide information of benefit to species conservation. We apply a bootstrapping procedure to citizen science data to reduce sampling biases and report the relative sensitivity of waterbird species to natural versus human‐dominated landscapes. Analyses are performed on 30,491 data records for 69 waterbird species referred to five functional groups observed in China between 2000 and 2018. Of these taxa, 30 species (43.5%) are significantly associated with natural landscapes, more so for cranes, geese, and ducks than for shorebirds and herons. The relationship between land association and the threat status of waterbirds is significant when the range size of species is considered as the mediator, and the higher the land association, the higher the threat status. Sensitive species significantly associated with natural landscapes are eight times more likely to be classified as National Protected Species (NPS) Classes I or II than less sensitive species significantly associated with human‐dominated landscapes. We demonstrate the potential for citizen science data to assist in conservation planning in the context of landscape changes. Our methods might assist others to obtain information to help relieve species decline and extinction.
Simple Wet Rendering Method for Extraction of Prime Quality Oil from Skipjack Tuna Eyeballs
Authors: Pudtikajorn Khamtorn , Benjakul Soottawat 
Published: June 2020

AbstractOil from skipjack tuna (ST) eyeballs is extracted by the wet rendering method at 121 °C for different holding times (5–60 min) using an autoclave. Yield increases as heating time increases up to 20 min (p < 0.05); no further increase is obtained with a longer heating time (p > 0.05). Conversely, acid value and total polar compounds increase. However, peroxide value (PV), thiobarbituric acid reactive substances, and anisidine value (AnV) decrease up to 30 min. No changes in unsaponifiable matter (UM) or conjugated diene (CD) are attained, regardless of heating time. Polar components (PC) increase with heating time (p < 0.05). All oil samples have a high polyunsaturated fatty acids (PUFA) content (40.46–41.00%), with monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) in the range of 20.94–21.26% and 37.77–38.45%, respectively. PUFA content is reduced slightly with a heating time of 60 min. Docosahexaenoic acid (DHA) (C22:6, n3) is the dominant fatty acid in all samples (31.67–32.24%). Based on FTIR spectra, heating for longer time results in lower intensity at wavenumbers of 3015 and 1740 cm−1. Thus, light yellow oil from ST eyeballs prepared by a green process for an appropriate time can serve as an excellent source of DHA and other PUFA.Practical Applications: Tuna oil is known to be a rich source of DHA and PUFA with health benefits. However, precooked tuna heads, generally used as raw material, yield oils with a very dark color and offensive odor, which require several refinery processes. To reduce the number of steps in the refinery process, tuna eyeballs which are separated from tuna heads can render higher quality fish oil. Moreover, the wet rendering process, a green process without toxic substances, can be used without causing environmental problems.
Surfactin production in the bioreactor: Emphasis on magnetic nanoparticles application
Authors: Modabber Glayol , Sepahi Abbas Akhavan , Yazdian Fatemeh , Rashedi Hamid 
Published: June 2020

AbstractSurfactin is one of the main lipopeptide biosurfactants produced by different species of Bacillus subtilis. This study aims to analyze the effect of starch‐coated Fe0 and Fe3+ nanoparticles on the biomass and biosurfactant production of Bacillus subtilis. Out of 70 soil samples, 20 Bacillus were isolated and genome sequenced by biochemical methods and 16S rRNA gene. Quantitative and qualitative screening methods were used to isolate and detect biosurfactant production. For the aim of this study, 61 and 63 (Bacillus subtilis subsp. Inaquosorum) were selected. Then, hemolytic activity, biomass amount, surfactant production, and reduction of surface tension in Minimal Salt Medium containing Fe0 and Fe3+ nanoparticles were examined after 48, 72, and 96 h of culture. Strain 61 was the best bacterium and Fe3+ was the best nanoparticle. The results were compared with the results of non‐nanoparticle bioreactor. The results showed the amount of biomass, surfactin, and surface tension decrease, 72 h after growth in 61 strain containing Fe3+ reached the highest values. Surfactin from strain 61 culture in the Fe3+nanoparticle bioreactor after 72 h of growth showed higher production than the same strain culture after 72 h without Fe3+, if continuing the research, this strain can be commercialized in the future.
Storage‐to‐wheels efficiency in a latest plug‐in electric vehicle
Authors: Boretti Alberto 
Published: June 2020

AbstractThe chemical, electrical, and mechanical power flow of a Chevrolet Spark plug‐in electric vehicle covering the cycles of the United States Environmental Protection Agency (US EPA), Vehicle Testing Regulations, namely the UDDS, Hwy and US06 cycles, is here analyzed. Experimental time series of instantaneous battery voltage and current are coupled to a simple model for energy conversion mechanical‐to‐electrical, and electrical‐to‐mechanical, and vice‐versa, and aerodynamic and rolling losses from Newton's equation model. A detailed CAE model is used to confirm the chassis dynamometer results complemented by the use of Newton's model. Computational time series of chemical, electric and mechanical power and energy are provided. Small advances are undergoing in motor/generator technology, where electric‐to‐mechanical efficiencies may approach 95%, while efficiencies mechanical‐to‐electric may exceed 90%. UDDS, Hwy and US06 efficiencies electric‐to‐wheels and wheels‐to‐electric are ηe−m = 0.794, 0.963, and 0.977, or ηm−e = 0.761, 0.893, and 0.902, respectively. Chemical‐to‐electric or electric‐to‐chemical efficiency ηc−e/e−c is about 0.984. These efficiencies are shared by the different products available on the market, with the weight of the vehicle and aerodynamic and rolling drags ultimately determining the energy efficiency of a specific vehicle.
The enhancement of security measures in advanced encryption standard using double precision floating point multiplication model
Authors: Srikanth B. , Kumar M. Siva , Ravindra J.V.R. , Kishore K. Hari 
Published: June 2020

AbstractThe multiplication strategy is a trending technology in chip programming model; also, it is used in different applications for different purpose. This research aims to develop the floating point double precision Furer Toom cook fast integer multiplication model in the Advanced Encryption Standard process round to enhance the security. In addition, the cryptography algorithms have been broken due to some harmful attack. So the main focus of this present research is to enhance the security measures in communication channel. Moreover, the proposed model is implemented in Xilinx and simulated in cadence 90 nm technology. By comparing the proposed work with recent existing models, the proposed research has reduced the power consumption as 5331.73 nW, thus 89% of power is reduced by the proposed Furer Toom cook multiplication model. The reduction of power enhanced the performance of multiplication algorithm so, while it is utilized in any application, it could not cause any loss of process. In addition, the developed strategy is process within 563 MHz frequency and utilized 11 250 cells. Thus, the proposed model provides better security for all wireless appliances.
Verhalten von Sulfatgestein im Tunnelbau – Erfahrungen aus Feldbeobachtungen und In‐situ‐Quelldrücke
Authors: Steiner Walter 
Published: June 2020

AbstractTunnels and underground works in sulphate bearing rocks are very challenging. For more than a century experience has accumulated in Switzerland and southern Germany with difficult conditions during construction and operation, which show the difficult conditions with extreme, long lasting heave of the invert and extreme swelling pressures. The analyses of published experience over more than a century and recent personal experience led to the compilation of many pieces of a puzzle and to a better understanding of complex processes. The swelling processes in sulphate bearing rocks are triggered by the formation of flow path for water, the dissolution, concentration and precipitation of minerals. In particular Anhydrite is dissolved and the solution becomes saturated, until precipitation and crystallization of Gypsum starts. The applied construction methods play an important influence on the rock mechanics and geochemical processes and finally the magnitude of in‐situ swelling pressures. The in‐situ swelling pressure are still large, but substantially less than the swelling pressures from laboratory tests. Estimated swelling pressures will be indicated.
From dust till dawn – Baubetriebliche Erfahrungen aus 14 Kilometer „Trockener Vortrieb”
Authors: Irmler Ralf , Türtscher Matthias , Hagenhofer Hannes , Schweiger Horst 
Published: June 2020

AbstractAs part of the reorganization of the Stuttgart railway node, many tunnels had to be driven through the rock formation of gypsum Keuper (Grabfeld Formation), which contains anhydrite. In contact with water, the mineral anhydrite converts into the mineral gypsum with a resulting increase of volume. To prevent damage to the tunnel structure and the buildings above the tunnel as a result of this swelling process, the tunnelling work had to be carried out dry. No water was allowed for cleaning, drilling or for dust reduction. Dry tunnelling for hundreds of metres in this form and intensity is a novelty. There were no empirical values. In this article the special features of a dry tunnelling are presented. A target‐actual comparison or geotechnical deformations should be deliberately avoided, although some peculiarities only result from changes to the contractual framework.
Lateral bearing behaviour of vibro‐ and impact‐driven large‐diameter piles in dense sand
Authors: Achmus Martin , Schmoor Kirill Alexander , Herwig Volker , Matlock Benjamin 
Published: June 2020

AbstractMonopiles are the preferred foundation solution for offshore wind turbines in sandy soils below shallow and moderate water depths. The piles are commonly installed using impact driving. A severe disadvantage of this installation method is that high noise levels occur in the water, which are a risk to animals living in the sea. Approval authorities are known to impose statutory requirements to mitigate this risk, placing a financial burden on installation. Vibratory driving is an alternative installation method that reduces noise emission levels significantly. However, the effect of vibro‐driving on the bearing behaviour of piles under lateral loading is largely unknown. Full‐scale load tests were conducted in predominantly dense, non‐cohesive, saturated soil to investigate the differences between impact‐driven piles and vibrated piles regarding their lateral bedding behaviour. CPTs and pile head load‐displacement curves were recorded and evaluated. In general, it was determined that the bearing behaviour of vibro‐driven piles is heavily dependent on the parameters of the installation process. For the installation parameters used in the test campaign, two vibro‐driven piles showed a weaker bedding reaction in comparison to the impact‐driven piles, although one vibrated pile installed with unintentionally different parameters showed an only slightly weaker behaviour under primary loading and even increased unloading and reloading stiffness. Furthermore, it was observed that an indication regarding the differences in the bedding resistance can be obtained via CPT measurements before and after installation, since the installation procedure considerably affects the relative density of the soil around the pile.
Rivaroxaban as an Alternative Agent for Heparin‐Induced Thrombocytopenia
Authors: Farasatinasab Maryam , Zarei Behnaz , Moghtadaei Mehdi , Nasiripour Somayyeh , Ansarinejad Nafiseh , Zarei Masoumeh 
Published: June 2020

AbstractHeparin‐induced thrombocytopenia (HIT) is a high‐risk adverse drug reaction because of its associated risk of life‐ and limb‐threatening thrombosis. Rivaroxaban may be considered as an ideal nonheparin anticoagulant alternative for the management of HIT. In this preliminary retrospective study, the efficacy and safety of rivaroxaban to control the clinically suspected HIT (4Ts score 4 points or greater) were evaluated. Patients with chronic kidney disease, hepatic impairment, mechanical heart valves, and active bleeding were excluded. Forty‐two eligible patients who received rivaroxaban for clinically suspected HIT were evaluated by medical records review, with 12‐month follow‐up after the first dose of rivaroxaban. End points included confirmed thrombosis (primary end point), mortality, and adverse treatment‐related events. HIT‐associated thrombosis was found in 17/42 (40.5%) patients before receiving rivaroxaban. After rivaroxaban therapy, platelet counts normalized in all patients, with only 1/42 (2.3%) patients developing new thrombosis. No hemorrhagic event was recorded in the patients. Twelve patients (28.6%) died, but the cause of death was not related to the thrombosis, hemorrhage, or adverse effects of rivaroxaban. Our findings are consistent with the available emerging data, suggesting that rivaroxaban is a safe and effective drug for the management of clinically suspected HIT. Rivaroxaban is a particularly valuable treatment option in developing countries, where there are issues of cost and availability of approved alternative agents.
Revisiting absent nasal bone in the second trimester
Authors: Singh Chanchal , Thakur Seema , Arora Nidhi , Khurana Deeksha 
Published: June 2020

AbstractPurposeTo evaluate the outcomes of fetuses diagnosed with absent nasal bone in the second trimester.MethodsThis prospective, observational study included all fetuses who were diagnosed at or referred to our fetal medicine center with an absent nasal bone from 16 weeks onwards from November 2017 to December 2019. Amniocentesis for fetal karyotype and microarray was offered to all women. Women who opted not to undergo invasive testing were also followed up and neonatal outcome noted.Results26 fetuses were eligible for inclusion in the study. 8 (30.8%) out of these were diagnosed with aneuploidy: 7 with trisomy 21 and one with trisomy 18. All fetuses with aneuploidy had additional ultrasound abnormality and/or high risk on biochemical screening.ConclusionsIsolated absent nasal bone in the second trimester with prior low risk on combined screening performed by certified sonographers is unlikely to be associated with Down syndrome.
Beyond bone biology: Lessons from team science
Authors: Zaidi Mone , Lizneva Daria , Gera Sakshi , Taneja Charit , Korkmaz Funda , Gumerova Anisa , Ievleva Kseniia , Ahmad Naseer , Ryu Vitaly , Sun Li , Kim Se‐Min , New Maria I. , Haider Shozeb , Iqbal Jameel , Rosen Clifford , Yuen Tony 
Published: June 2020

AbstractToday, research in biomedicine often requires the knowledge and technologies in diverse fields. Therefore, there is an increasing need for collaborative team science that crosses traditional disciplines. Here, we discuss our own lessons from both interdisciplinary and transdisciplinary teams, which ultimately ushered us to expand our research realm beyond bone biology.
Property changes of caseinate in response to its dityrosine formation induced by horseradish peroxidase, glucose oxidase and d‐glucose
Authors: Li Rui , Chang Chun‐Hui , Ma Dan‐Dan , Zhao Xin‐Huai 
Published: June 2020

AbstractBACKGROUNDA ternary system containing horseradish peroxidase (HRP), glucose oxidase and d‐glucose using one‐ or two‐step treatment was evidently able to cross‐link proteins via dityrosine formation and thus was assessed for its possible impact on several properties of a protein ingredient caseinate.RESULTSHRP, glucose oxidase and d‐glucose were used at 200 U, 6 U and 0.05 mmol g−1 protein to treat caseinate by one‐ and two‐step methods, producing two cross‐linked caseinates named CLCN‐I and CLCN‐II, respectively. In response to the conducted cross‐linking, both CLCN‐I and CLCN‐II gained slightly reduced dispersibility at pH 5–10, enlarged hydrodynamic radius (particle size distribution, 266.37 and 258.33 versus 226.67 nm) and negative zeta‐potential (−26.60 and −22.27 versus −14.30 mV) in dispersions, increased water‐binding (3.70 and 3.09 versus 2.68 kg kg−1 protein), decreased oil‐binding (1.75 and 2.74 versus 2.87 kg kg−1 protein) and emulsifying activity (76.2 and 82.3 versus 94.3 m2 g−1 protein), increased emulsion stability (84.3% and 82.5% versus 78.6%), and enhanced thermal stability with lower mass loss (58.5% and 59.6% versus 64.3%) or higher decomposition temperatures (331.2 °C and 328.7 °C versus 327.6 °C) upon heating at 105–450 °C. In addition, CLCN‐I and CLCN‐II had decreased gelling temperatures and shortened gelling times when forming acid‐induced gels, and the gels were endowed with increased values in four textural indices and finer microstructure. Moreover, CLCN‐I with a higher cross‐linking extent showed greater property changes than CLCN‐II.CONCLUSIONThis ternary system could be used in caseinate cross‐linking to improve properties such as aggregation, emulsification, gelation and thermal stability. © 2020 Society of Chemical Industry
Nephroprotective effects of chlorogenic acid against sodium arsenite‐induced oxidative stress, inflammation, and apoptosis
Authors: AL‐Megrin Wafa A , Metwally Dina M , Habotta Ola A , Amin Hatem K , Abdel Moneim Ahmed E , El‐khadragy Manal 
Published: June 2020

AbstractBACKGROUNDChronic exposure to arsenic (As) leads to serious renal disorders. Chlorogenic acid (CGA), a phenolic compound, has several well known physiological benefits, including antioxidant and anti‐inflammatory activities. The present study investigated the potential renoprotective effects of CGA on sodium arsenite (NaAsO2)‐induced kidney damage in mice. The mice were randomly allocated into five groups to receive daily treatment with CGA (200 mg kg−1), NaAsO2 (5 mg kg−1), NaAsO2 + CGA (100 mg kg−1), NaAsO2 + CGA (200 mg kg−1), or a control for 28 days.RESULTSIn the NaAsO2‐treated group, NaAsO2 induced significant renal dysfunction, oxidative damage, inflammation, and apoptosis, as demonstrated by marked increases in urea and creatinine levels accompanied by a decrease in the kidney index. Considerable increases in malondialdehyde and nitric oxide levels and parallel decreases in various antioxidant markers (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione) levels were also detected in the renal tissues of NaAsO2‐treated mice. NaAsO2 exposure was associated with marked increases in renal inflammatory markers (interleukin‐1β and tumor necrosis factor‐α) and apoptosis indicators including Bax and caspase‐3 levels contaminant, with a marked decrease in Bcl‐2, an anti‐apoptotic protein, in the NaAsO2‐treated group compared with the control group. However, pretreatment with CGA substantially mitigated the renal injury and dysfunction associated with NaAsO2 exposure by reducing tissue inflammation and apoptosis and improving the antioxidant status. The CGA pretreatment also alleviated the NaAsO2‐induced histological alterations in renal tissues.CONCLUSIONTaken together, our results suggest the efficacy of CGA in alleviating As‐mediated renal tissue damage. © 2020 Society of Chemical Industry