bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing Fetal Movements in Pregnancy: A Qualitative Evidence Synthesis of Women’s Views, Perspectives and Experiences
Authors: Smith Valerie , Muldoon Kathryn , Brady Vivienne , Delaney Hannah 
Published: November 2020

Abstract
Background: Raising awareness of the importance of fetal movements (FMs) and advising women on the appropriate action to take if they experience reduced FMs, is critical for minimising or avoiding adverse perinatal outcomes. To gain insight and understanding of women’s perspectives of assessing FMs in pregnancy, we conducted a qualitative evidence synthesis. Methods: A qualitative evidence synthesis using thematic synthesis was conducted. Studies were eligible if they included pregnant women who were at least 20 weeks gestation and reported qualitative data from women on assessing FMs in pregnancy. MEDLINE, CINAHL, EMBASE, PsycINFO and Social Science Citation Index, from inception to July 2020, were searched. The methodological quality of included studies was assessed by at least two reviewers using an EPPI-Centre quality assessment tool. Data analysis, using the Thomas and Harden framework, involved line by line coding of extracted data, establishing descriptive themes, and determining analytical themes. Confidence in the findings was assessed using GRADE CER-Qual. Results: Nine studies, involving 2193 women, were included in the review. The methodological quality of the studies was overall generally high. The synthesis revealed three dominant themes, and seven sub-themes that reflected women’s perspectives of assessing FMs in pregnancy. These were; 1) How women engage with FMs, with subthemes of informal engagement, formal engagement, and strategies to stimulate FMs; 2) ‘…like a feather inside my belly’ - articulating and describing FMs, with sub-themes of sensations associated with FMs and timing and frequency of FMs; and 3) FMs and help/health seeking, with sub-themes of information sources and interacting with healthcare professionals. Confidence in the findings was either high or moderate, although two findings were rated low confidence and one very low.Conclusion: This qualitative evidence synthesis reveals that women informally engage with FMs during pregnancy. Women commonly adopt strategies to stimulate FMs when concerned. The use of the internet was a common source of obtaining information regarding FMS. Women require better support when contacting healthcare professionals about FMs. As only three of the nine included studies were exclusively qualitative in design, further qualitative studies exploring women’s perspective of assessing FMs in pregnancy are required.
Investigation of Immune Microenvironment in Children with Langerhans Cell Histiocytosis
Authors: Zhang Fuyong , Wang Weihong , Zhen Yunfang , Dai Jin , Zhu Lunqing , Liang Peirong , Su Guanghao , Zhang Min , Fang Jianfeng , Yuan Quanwen , Liu Ya , Zhuang Ting , Wang Xiaodong , Liu Yao 
Published: November 2020

Abstract
Background: Langerhans cell histiocytosis (LCH) is a rare disease that mainly occur in children. The aim of this investigation is to explore the immune microenvironment of LCH and feasibility of immunotherapy for children with LCH. Methods: Tissue samples were collected from 15 children with LCH and their clinical characteristics were recorded. The expressions of PD-1 ligand 1(PD-L1) and the presence of CD8 T lymphocytes were assessed by immunohistochemistry (IHC). Results: Of the total 15 patients, 8 of them were PD-L1 positive and it accounted for 53.33% and 9 of them were CD8 T lymphocytes positive and it accounted for 60%. There were 8 out of the 15 patients that were both PD-L1 positive and CD8 positive and they accounted for 53.33%.Conclusions: Our results showed that the expression of PD-L1 and presence of CD8 T lymphocytes occurred in the microenvironment of LCH. The findings indicated a possible new treatment option for LCH in children.
Analyzing the value of NUSAP1 in hepatocellular carcinoma from clinical and molecular mechanism perspectives: Bioinformatics-based approach.
Authors: Zhu Wenjie , Wang Jie , Chen Zehao , Jiang Jianxin 
Published: November 2020

Abstract
Background: Hepatocellular carcinoma (HCC) is one of the most common and highest mortality rates carcinomas worldwide. At present, there are varies of therapeutic methods can be provided for HCC patients. However, there is no one method can inhibit occurrence and development of HCC very well, and prognosis of HCC patients is still very poor. Herein, our study aims to identify a key gene closely related to occurrence, development, poor prognosis of HCC and explore its underlying mechanism from molecular level.Methods: GSE62232, GSE102079, GSE112790 and GSE121248 genes expression profile datasets were screened from Gene Expression Omnibus (GEO) database. R studio was used to identify DEGs of each dataset. Venn online tool was used to generate a Venn diagram and screen overlapping DEGs of the four datasets. Search Tool for the Retrieval of Interacting Genes (String) online tool was used to draw Protein–Protein Interaction (PPI) network. And the most significant module and the key gene NUSAP1 in PPI network were identified by MCODE and cytoHubba plug-in in Cytoscape software. Oncomine database and Kaplan–Meier Plotter database were used to analyze relationships between expression of NUSAP1 and occurrence, development, prognosis of HCC. The cBioPortal online tool was used to identify co-expression genes of NUSAP1 in HCC patients from TCGA database. Then, KEGG pathway analysis was carried out by DAVID online tool and Cell cycle pathway map was generated by Kyoto Encyclopedia of Genes and Genomes (KEGG) online tool.Results: A total of 86 overlapping DEGs were screened, which included 55 up-regulated DEGs and 31 down-regulated DEGs. Then the key gene NUSAP1 in the PPI network were screened using cytoHubba plug-in in Cytoscape software. We found NUSAP1 may be associated with occurrence, development and poor prognosis of HCC by analyzing HCC patients in Oncomine database and Kaplan–Meier Plotter. Co-expression genes of NUSAP1 in TCGA database were obtained by cBioPortal. And KEGG pathway analysis was produced using the top 300 co-expression genes of NUSAP1, the result showed most co-expression genes closely related to the expression of NUSAP1 were concentrated in Cell cycle. Thus, we generate a KEGG pathway map of Cell cycle and found that most of these genes were located in S phase and G2/M phase of the Cell cycle and they could regulate the genes in G1 phase, hence, we inferred that NUSAP1 may regulate the progression of HCC by promoting the transition from the G1 phase to the S phase.Conclusion: NUSAP1 may influence occurrence, development and prognosis of HCC and might be a new molecular marker in HCC.
ANGPTL4 supports glutamine metabolism and promotes fatty acid oxidation in nonsmall cell lung cancer cells
Authors: Xiao Song , Nai-Dong Wang , Yan Jin-Xiang , Tian Long , Lu Xiu-Rong , Gao Hong , Yan Jie-Cheng , Zhang Fei 
Published: November 2020

Abstract
Background Angiopoietin-like proteins (ANGPTLs) 4 is key factor in the regulation of lipid and glucose metabolism in metabolic diseases. It also has been demonstrated highly expressed in cancers, but the regulation of energy metabolism on tumor remains to be determined.Methods Two different NSCLC cells A549 and H1299 were used to investigate the role of ANGPTLs 4 in energy metabolism by tracer technique and seahorse XF technology in ANGPTLs4 knockdown cell. RNA microarray and specific inhibitors were used in identification targets in overexpression ANGPTLs4 cells.Results Knockdown ANGPTLs4 could inhibit the energy metabolism and proliferation of NSCLC cells. Knockdown ANGPTLs4 had no significant effect on glycolysis, but affected the glutamine consumption and fatty acid oxidation. Knockdown ANGPTLs4 also significantly inhibited the growth of metastasis and energy metabolism of tumor in mice, but had a weak effect on glycolysis. RNA microarray analysis showed that ANGPTLs4 significantly affected GLS and CPT1. ANGPTLs4 overexpression cells exposed to glutamine deprivation, the effect on cell proliferation and energy metabolism were significantly decreased, but still has difference compared with normal NSCLC cells. ANGPTLs4 overexpression cells treated with GLS and CPT1 inhibitor at the same time, the regulatory effects on cell proliferation and energy metabolism were disappeared.Conclusions ANGPTLs4 could promote the glutamine consumption and fatty acid oxidation, but not glycolysis, and accelerated the energy metabolism in NSCLC.
Saline collection media and an extraction free workflow enables massively scalable, highly sensitive, and cost-effective SARS-CoV-2 testing
Authors: Lim Yan Wei , Leonetti Nicole , Amin Aakash , Machado Henrique , Bonilla Natasha , Lu James , Becker K.David 
Published: November 2020

Abstract
The challenges in scaling up SARS-CoV-2 testing capacity include shortages in the supply chain for consumables and reagents. Improvements in consumption patterns can be obtained through removal of key processing steps, including RNA extraction. Here, we present a scalable and validated extraction-free method for the detection of SARS-CoV-2 from swab specimens in saline, with a limit of detection at 1,000 GCE/mL and a sensitivity and specificity of 100%.
Larval habitats of Anopheles gambiae senso lato (Diptera-Culicidae) in Anger Gute resettlement villages, western Ethiopia
Authors: Kenea Oljira , Dufera Mebrate 
Published: November 2020

Abstract
Background: Anger Gute is one of the national malaria surveillance Sentinel Sites in Ethiopia targeted to generate malaria data for the disease control, elimination and eradication. The objective of this work was to obtain information about the habitat characterization of An. gambiae s.l. larvae in western Ethiopian lowlands particularly in Anger Gute resettlement villages. Methods: A longitudinal mosquito larval habitat survey was undertaken every month from January to December 2018. The study covered three rural farming villages namely Tulu Lencha, Warabo and Dalasa Makanisa. All anopheline positive larval habitats present within a 500 m radius of each village and 700 m along the major streams which were located adjacent to the villages were sampled year round to study the mosquito larval density and the habitat characteristics. Larval collections were done from various aquatic habitats in all the villages using the standard dipping method. All III and IV instar anopheline larvae collected were preserved in 70% alcohol. In Wollega University Entomology laboratory, each larva was individually mounted on a microscope slide and identified to An. gambiae s.l. by morphological criteria using identification keys for the Ethiopian mosquitoes. Characteristics of the larval habitats were determined using mean comparison of the mosquito larval density using one-way analysis of variance (ANOVA). Results: The mosquito larvae were found in many diverse habitats and were collected most abundantly from rain pool puddles (35.0%) followed by river edge pools (23.5%). During wet season, most of (83.3%) the larval habitats were accessed along roads. Whereas during dry season the habitats were most accessible along streams in the study localities. Significantly higher mean densities of the mosquito larvae were obtained from aquatic habitats that had turbid standing water and in habitats near to human dwellings (<500 m). Conclusions: The malaria mosquito An gambiae s.l. breeds most abundantly in rain pool puddles along footways and roads closer to human habitation during wet season and in stream edges along local streams in dry season in Anger Gute Resettlement villages. These findings suggest that targeting malaria mosquito larval intervention along footways and roads in wet season and on stream edges along local streams in the dry season could result in effective larval control of the malaria vector in the study setting.
Patterns and Effects of Admission Hyperglycemia And Inflammatory Response in Trauma Patients: A Prospective Study
Authors: El-Menyar Ayman , Asim Mohammad , Mir Fayaz , Hakim Suhail , Kanbar Ahad , Siddiqui Tariq , Younis Basil , Ahmed Khalid , Mahmood Ismail , Atique Sajid , Jogol Hisham Al , Taha Ibrahim , Mustafa Fuad , Alabdallat Mohammad , Abdelrahman Husham , Peralta Ruben , Al-Thani Hassan 
Published: November 2020

Abstract
Background: Hyperglycemia following trauma could be a response to stress. The constellation of the initial hyperglycemia, proinflammatory cytokines and severity of injury among trauma patients is understudied. We aimed to evaluate the patterns and effects of on-admission hyperglycemia and inflammatory response in a level 1 trauma center admissions. Methods: A prospective, observational study was conducted for adult trauma patients who were admitted and tested for on-admission blood glucose, hemoglobin A1c, interleukin (IL)-6 ,Il-18 and hs-CRP. Patients were categorized into 4 groups (non-diabetic normoglycemic, diabetic normoglycemic, diabetic hyperglycemic (DH) and stress-induced hyperglycemic (SIH)). The inflammatory markers were measured on 3 time points (admission, 24 h, and 48 h). Pearson’s correlation test and logistic regression analysis were performed. We hypothesized that higher initial readings of blood glucose and cytokines are associated with severe injuries and worse in-hospital outcomes in trauma patients.Results: During the study period, 250 adult trauma patients were enrolled. Almost 13% of patients presented with hyperglycemia (SIH&DH); of whom 50% had SIH. Compared to the other 3 groups; SIH patients were younger, had significantly higher ISS, higher IL-6 readings, prolonged hospital length of stay and higher mortality. The SIH group had lower Revised Trauma Score (p=0.005), lower Trauma Injury Severity Score (p=0.01) and lower GCS (p=0.001). IL-18 and hs-CRP were comparable among the study groups. Compared to the normoglycemia groups, patients with hyperglycemia had elevated high- sensitive troponin T (p=0.001) and required more blood transfusion (p=0.03). Patients with hyperglycemia had 3-times higher in-hospital mortality than the normoglycemia groups (p=0.02). A significant correlation was identified between initial blood glucose and serum lactate, IL-6, ISS and hospital length of stay. IL-6 correlated well with ISS (r=0.40, p=0.001). On- admission blood glucose had age-sex-GCS adjusted odd ratio 1.20(95% CI 1.06-1.33, p=0.003) for severe injury (ISS≥16).Conclusions: On-admission hyperglycemia is associated with a significant severer injury than normoglycemia patients. Initial blood glucose correlates with serum IL-6 which indicates a potential role of the systemic inflammatory response in the disease pathogenesis among the injured patients. On-admission glucose level could be a useful marker of injury severity, triage and risk assessment in trauma patients.This study was registered at the ClinicalTrials.gov (Identifier: NCT02999386), retrospectively Registered on December 21, 2016 https://clinicaltrials.gov/ct2/show/NCT02999386.
Trends in reasons for emergency calls during the COVID-19 crisis in the department of Gironde, France using automatic classification.
Authors: Gil-Jardiné Cédric , Chenais Gabrielle , Pradeau Catherine , Tentillier Eric , Revel Philipe , Combes Xavier , Galinski Michel , Tellier Eric , Lagarde Emmanuel 
Published: November 2020

Abstract
Objectives During periods such as the COVID-19 crisis, there is a need for responsive public health surveillance indicators related to the epidemic and to preventative measures such as lockdown. The automatic classification of the content of calls to emergency medical communication centers could provide relevant and responsive indicators. Methods We retrieved all 796,209 free-text call reports from the emergency medical communication center of the Gironde department, France, between 2018 and 2020. We trained a natural language processing neural network model with a mixed unsupervised/supervised method to classify all reasons for calls in 2020. Validation and parameter adjustment were performed using a sample of 20,000 manually-coded free-text reports. Results The number of daily calls for flu-like symptoms began to increase from February 21, 2020 and reached an unprecedented level by February 28, 2020 and peaked on March 14, 2020, 3 days before lockdown. It was strongly correlated with daily emergency room admissions, with a delay of 14 days. Calls for chest pain, stress, but also those mentioning dyspnea, ageusia and anosmia peaked 12 days later. Calls for malaises with loss of consciousness, non-voluntary injuries and alcohol intoxications sharply decreased, starting one month before lockdown. Discussion This example of the COVID-19 crisis shows how the availability of reliable and unbiased surveillance platforms can be useful for a timely and relevant monitoring of all events with public health consequences. The use of an automatic classification system using artificial intelligence makes it possible to free itself from the context that could influence a human coder, especially in a crisis situation. Conclusion The content of calls to emergency medical communication centers is an efficient epidemiological surveillance data source that provides insights into the societal upheavals induced by a health crisis.
Sigmoid colon perforation caused by three living loaches inserted into anus: a case report
Authors: Li Jun , Zhao Ning , Pang Kai , Zhang Tingting , Wu Yadong , An Yongbo , Wu Guocong , Yang Yingchi , Yin Jie , Wang Jin , Zhang Jun , Jin Lan , Yao Hongwei , Zhang Zhongtao 
Published: November 2020

Abstract
BACKGROUND Colorectal perforation are common surgical emergencies. Yet perforation caused by living animals has not been reported with no record found on Medline. CASE PRESENTATION A 47-year-old male arrived at our surgical emergency room for "severe abdominal pain with itchy rash for 5 hours", who explained that "three big living loaches" had been squeezed into the anus 1 hour before the onset of symptoms. Thorough physical and auxiliary examinations suggested lower gastrointestinal perforation. After prompt anti-shock, anti-allergy, analgesia and gastrointestinal decompression, we performed exploratory laparotomy, sigmoid colon repair and double-lacuna ileostomy. The patient underwent uneventful postoperative recovery and was discharged on the 10th day after surgery. CONCLUSIONS CT examination is critical for diagnosing colorectal perforation caused by living animals (3 loaches as reported in our case). Specifically, due attention should be given to the serious allergic reaction induced by exogenous organisms entering the abdominal cavity. Surgery should be performed as soon as possible to fundamentally address the allergic shock. As to colorectal perforation caused by multiple living organisms, considering the restricted condition for thorough exploration during emergency surgery (particularly the exploration of the posterior wall of the colon), colostomy should be selected with caution.
Pretreatment with LCK Inhibitors Chemosensitizes Cisplatin-Resistant Endometrioid Ovarian Tumors
Authors: Crean-Tate Katie , Braley Chad , Dey Goutam , Esakov Emily , Saygin Caner , Trestan Alexandria , Silver Daniel , Turaga Soumya M. , Connor Elizabeth V. , Debernardo Robert , Michener Chad M. , Rose Peter G. , Lathia Justin , Reizes Ofer 
Published: November 2020

Abstract
Background: Ovarian cancer is the most fatal gynecologic malignancy in the United States. While chemotherapy is effective in the vast majority of ovarian cancer patients, recurrence and resistance to standard systemic therapy is nearly inevitable. We discovered that CD55 via activation of the non-receptor tyrosine kinase Lymphocyte Cell-Specific Protein-Tyrosine Kinase (LCK) promoted cisplatin resistance. Here, we hypothesized that treating high grade, platinum resistant endometrioid cancer cells with an LCK inhibitor (LCKi) followed by co-treatment with cisplatin would lead to increased cisplatin efficacy. Our objective was to assess clinical outcomes associated with increased LCK expression, test our hypothesis of utilizing LCKi as pre-treatment followed by co-treatment with cisplatin in platinum resistant ovarian cancer in vitro, and evaluate our findings in vivo to assess LCKi applicability as a therapeutic agent. Results: Kaplan-Meier (KM) plotter data indicated LCK expression is associated with significantly worse median progression-free survival (HR 3.19, p=0.02), and a trend toward decreased overall survival in endometrioid ovarian tumors with elevated LCK expression (HR 2.45, p=0.41). In vitro, cisplatin resistant ovarian endometrioid cells treated first with LCKi followed by combination LCKi-cisplatin treatment showed decreased cell viability and increased apoptosis. Immunoblot studies revealed LCKi led to increased expression of phosphorylated H2A histone family X (-H2AX), a marker for DNA damage. In vivo results demonstrate treatment with LCKi followed by LCKi-cisplatin leads to significantly slowed tumor growth.Conclusions: We identified a strategy to therapeutically target cisplatin resistant endometrioid ovarian cancer leading to chemosensitization to platinum chemotherapy via treatment with LCKi followed by co-treatment with LCKi-cisplatin.
The role of computed tomography in predicting peritoneal carcinomatosis and upfront surgery outcome in advanced ovarian cancer
Authors: Asp Mihaela , Malander Susanne , Wallengren NilsOlof , Pudaric Sonja , Bengtsson Johan , Sartor Hanna , Kannisto Päivi 
Published: November 2020

Abstract
Background Epithelial ovarian cancer is usually diagnosed at advanced stages. To choose the best therapeutic approach, an accurate assessment of the tumor spread is crucial. This study aimed to determine whether numeric scoring, the amount of ascites, and the presence of cardiophrenic nodes (CPLNs) visualized by computed tomography (CT), can predict the tumor extent and improve the outcome of AOC upfront surgery. Methods This single center retrospective analysis of 194 patients diagnosed with AOC included 119 patients treated with upfront surgery at the Skåne University Hospital, Lund, Sweden, from January 2016 to December 2018. CT based peritoneal cancer index (PCI) scores, enlarged cardiophrenic lymph nodes (CPLNs), and the amount of ascites were correlated to the surgical PCI (S-PCI) and the completeness of the cytoreductive surgery.The patients were grouped according to the residual disease (RD) and the overall survival (OS) rates for the three groups were determined using Kaplan-Meier curves. Linear regression and the interclass correlation (ICC) analyses were used to determine the relationship between CT-PCI and S-PCI. Results The survival rate was significantly higher in patients with no macroscopic residual disease compared those with residual disease <10 mm (p<0.03) or residual disease ≥10 mm (p<0.005). S-PCI and large ascites volumes were correlated with the risk of suboptimal residual disease (for ascites > 1000 ml, OR 5.5626 (1.665-19.007) p<0.019; for S-PCI, OR 1.24 (1.141-1.348), p<0.001). CT-PCI, CA-125 level and CPLN were not predictive of the cytoreductive surgery results in the adjusted data to days from CT to operation and for ascites. CT-PCI correlated well to S-PCI ((95%) CI: 0.397 (0.252-0.541) p<0.001). Conclusions CT is a reliable tool for assessing the extent of the disease in AOC, but it has limitations in predicting surgical outcome. This study was unable to show an association between the CT-PCI and surgical outcome when the data were adjusted and ascites, CA-125 level, days between the CT examination to surgery and CPLN. Ascites volumes exceeding 1000 ml increased the risk of residual disease and thereby worse outcome. That certain areas (e.g., small bowel region) are particularly critical when evaluating surgical outcome using preoperative CT-PCI warrants further investigation.