bluuebunny/crossref_metadata_2025_split huggingface dataset:
Local anesthetics impair the growth and self-renewal of glioblastoma stem cells by inhibiting ZDHHC15-mediated GP130 palmitoylation
Authors: Fan Xiaoqing , Yang Haoran , Zhao Chenggang , Hu Lizhu , Wang Delong , Wang Ruiting , Fang Zhiyou , Chen Xueran 
Published: October 2020

Abstract
BackgroundA large number of preclinical studies have shown that local anesthetics have a direct inhibitory effect on biological activities, including cell survival, proliferation, migration, and invasion. There are few studies on the role of local anesthetics in cancer stem cells. This study aimed to determine the possible role of local anesthetics in glioblastoma stem cell (GSC) self-renewal and the underlying molecular mechanisms.MethodsThe effects of local anesthetics in GSCs were investigated through in vitro and in vivo assays (i.e., cell counting kit 8, spheroidal formation assay, double immunofluorescence, western blot, and xenograft model). The Acyl biotin exchange method (ABE) assay was identified proteins that are S-acylated by zinc finger Asp-His-His-Cys-type palmitoyltransferase 15 (ZDHHC15). Western blot, co-immunoprecipitation, and liquid chromatograph mass spectrometer-mass spectrometry assays were used to explore the mechanisms of ZDHHC15 in effects of local anesthetics in GSCs.ResultsIn this study, we identified a novel mechanism through which local anesthetics can damage the malignant phenotype of glioma. We found that local anesthetics prilocaine, lidocaine, procaine, and ropivacaine can impair the survival and self-renewal of GSCs, especially the classic glioblastoma subtype. These findings suggest that local anesthetics may weaken ZDHHC15 transcripts and decrease GP130 palmitoylation levels and membrane localization, thus inhibiting the activation of IL-6/STAT3 signaling. ConclusionsIn conclusion, our work emphasizes that ZDHHC15 is a candidate therapeutic target, and local anesthetics are potential therapeutic options for glioblastoma.
SOX Chemotherapy is Effective Treatment for Gastric Cancer with Liver Metastases: A Case Report
Authors: Pan Jiyong , Li Guangyao , Zhou Jialin , Wu Peiliang , Fan Zhe 
Published: October 2020

Abstract
Background: Gastric cancer is a malignant tumor of global significance. Many patients have been shown to have advanced gastric cancer (AGC) with liver metastases, and thus are not surgical candidates. However, there is not report about hepatic metastasis disappearing.Case presentation: Herein we report a patient of AGC with liver metastases who received SOX (tegafur gimeracil, oteracil, and oxaliplatin) chemotherapy and the liver metastases regressed. Conclusions: SOX is effective in the treatment of AGC.
The Potential Effect of Nav1.8 in Autism Spectrum Disorder: Evidence from A Congenital Case with Compound Heterozygous SCN10A Mutations
Authors: Heinrichs Björn , Liu Baowen , Zhang Jin , Meents Jannis , Le Kim , Hautvast Petra , Zhu Xiwen , Li Ningbo , Liu Yi , Rothermel Markus , Namer Barbara , Zhang Xianwei , Lampert Angelika , Duan Guangyou 
Published: October 2020

Abstract
BackgroundApart from the most prominent symptoms in Autism spectrum disorder (ASD), namely deficits in social interaction and repetitive behavior, patients often show abnormal sensory reactivity to environmental stimuli. Especially potentially painful stimuli are reported to be experienced in a different way compared to healthy persons.MethodsIn our present study, we present an ASD patient carrying compound heterozygous mutations in the voltage-gated sodium channel (VGSC) Nav1.8, which is preferentially expressed in sensory neurons. We expressed both identified mutations, p.I1511M and p.R512X, in a heterologous expression system and investigated their biophysical properties using patch-clamp recordings. ResultsThe results of these experiments suggest that both mutations lead to different degrees of loss-of-function of Nav1.8. Behavioral experiments in a Nav1.8 loss-of-function mouse model additionally revealed Nav1.8 may play a role in autistic behavior. LimitationsOur study did not verified the functions of p.I1511M and p.R512X mutations in vivo. The mice with these mutations can be constructed to verify the mutation functions in the future investigations. In addition, since we have only found one ASD patient which may relate to SCN10A mutations, the role of Nav1.8 in ASD needs to be confirmed in more cases. Moreover, the cellular mechanism underlying the effect of Nav1.8 on ASD needed to be explored in future studies.ConclusionsOur results present Nav1.8 as a protein potentially involved in ASD pathophysiology and may therefore offer new insights to the genetic basis of this disease.
Wolbachia Endosymbiant Was Not Detected in Field Collected Population of Head and Body Lice From Iran Using Molecular Markers
Authors: Firooziyan Samira , Sadaghianifar Ali , Khaligh Fereshteh Gahvehchi , Oshaghi Mohammad Ali , Gholizadeh Saber 
Published: October 2020

Abstract
Objectives Head lice infection can lead to inferiority, depression, insomnia, and lack of education, loss of social status, secondary infections, hair removal, and allergies. Body lice can carry dangerous diseases such as typhus, trench fever, and recurrent fever. Studies on lice control due to pesticides, inappropriate drug use, pesticide risks, and pesticide resistance in the lice population are needed. The study of insect symbiotic microorganisms such as Wolbachia is a new approach to control the vector-borne disease. ResultsThe presence of this bacterium was investigated in head and body lice collected from the Iranian lice population. Genomic DNA was extracted from both lice specimens and PCR assay was performed to the detection of Wolbachia infection by using Wsp, coxA, and gatB primers. All PCR results were negative for Wolbachia in comparison with positive controls. Further studies should be performed using new molecular markers to determine the Wolbachia infection of lice.
The Effect of Trunk Muscle Exercise on Cardiopulmonary Resuscitation
Authors: yang hyunmo , Kim Gyoungyong , Kim Jinwoo , Kim Yongseok 
Published: October 2020

Abstract
Background: The purpose of this study is to find how trunk muscles mainly used in cardio-pulmonary resuscitation, including pectoralis major, rectus abdominis, and erector spinae, influence the result of 8-minute when the muscles have six-weeks plank exercise, and to analyze the duration time of exercise effect. Methods: Study subjects were 30 participants taking a relevant course for 24 months. After they had plank exercise for six weeks, they didn't have any exercises including plank exercise for six weeks. As such, the students participated in the 12-weeks program. For chest compression simulation, a practictical mannequin was used. Without any respiration, a single rescuer continued to perform only chest compression for 8 minutes. Results: Regarding their change in a muscle mass by plank exercise, there was a statistically significant difference in the change from 4th to 8th week after the program (p<.01). The muscle activity change had a statistically significant difference from 3rd to 10th week (p<.01). The chest compression depth had a statistically significant difference from 4th to 8th week (p<.01). The chest compression rate didn't had a statistically significant difference for 12 weeks. In addition, insufficient chest relaxation height after compression had a statistically significant difference from 4th to 10th week(p<.01). The chest compression maintenance time had a statistically significant difference from 2nd to 12th week (p<.01). Conclusion: If the study subjects have three sets of plank exercise program for five weeks, which consisted of one set of 10 times of 30-second motion and 20-second rest and does not have up to two weeks of detraining, the plank exercise is considered to be helpful for them in out of hospital cardiac arrest at the time when there is no use of mechanical chest compression device.Trial registration: The study was registered on 12/15/2015 in the Chungnam National University Hospital with number 2015-10-035-005.
A Retrospective Study on the Efficacy of PAH-targeted Therapy in Patients with Severe Pulmonary Hypertension in Chronic Lung Disease
Authors: jieying li , zhang sun , Chen haiming , Guo wenliang , Liu chunli , Hong Cheng 
Published: October 2020

Abstract
Background Using PAH-targeted therapy in severe pulmonary hypertension (PH) patients with chronic lung disease (CLD) is controversial as the pulmonary vasodilation could worsen hypoxemia. Objective To investigate the efficacy of patients with severe PH-CLD to PAH targeted therapy. Methods We retrospectively enrolled patients with severe PH-CLD in our study. Each patient underwent laboratory indicators and transthoracic echocardiography at baseline and after the therapy, and survival after therapy were also evaluated. Results After PAH targeted therapy, NT - proBNP significantly decreased (1043.10(236.75-2697.50) pg/ml vs 287.10(21.00-555.90) pg/ml, p=0.035), whereas PaO2 and the oxygenation index in blood gas analysis significantly increased(59.18± 30.12 mmHg vs 74.35(61.63-163.95)mmHg, p=0.010 and 177.21± 84.38 mmHg vs 286.22± 165.13 mmHg, p=0.035, respectively). But the difference at 1-,3- years survival rates between the two groups was not statistically significant (82.82% and 66.26% vs 75.87% and 20.23%, respectively, p=0.094). Multivariate regression analysis identified baseline mean right atrial pressure values and cardiac index as significant predictors of survival of patients with severe PH-CLD. Conclusions PAH targeted therapy may have benefit in patients with severe PH-CLD and didn’t worsen their hypoxemia. Right ventricular function might have an important impact on the survival of patients.
The Protective Effect of Dietary Administration of Puerarin on Canine Osteoarthritis Model With Anterior Cruciate Ligament Transected
Authors: Ma Tianwen , Wen Yajing , Song Xiaopeng , Hu Hailong , Li Yue , Zhao Mingchao , Bai Hui , Chen Hong , Gao Li 
Published: October 2020

Abstract
BackgroundLameness caused by osteoarthritis (OA) is one of the main causes of disability in elderly dogs. Non-steroidal anti-inflammatory drugs (NSAIDs) are important tools in the treatment of canine OA. In recent years, due to the many side effects of NSAIDs, patients cannot tolerate or do not want to take the risk of NSAIDs. People are becoming more and more interested in new treatments for canine OA, and so-called nutritional supplements have emerged. Puerarin has a wide range of pharmacological activities and is often used as a clinical prescription drug and dietary supplement in China. However, the effect of puerarin on canine OA has not been evaluated. Therefore, the purpose of this study is to evaluate the anti-inflammatory and anti-cartilage degradation effects of puerarin in a canine OA model induced by anterior cruciate ligament transection (ACLT), and to detect the serum inflammatory factor interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) levels and cartilage degradation biomarker C-terminal telopeptides of collagen type II (CTX-II), cartilage oligomeric matrix protein (COMP) and chondroitin sulfate 846 epitope (CS 846) levels in serum and synovial fluid at different periods of puerarin administration. ResultsEight weeks after the administration, the veterinarian performed clinical and imaging evaluations to comprehensively evaluate the protective effect of puerarin on canine OA. Daily oral administration of 20 mg/kg puerarin can significantly inhibit the expression of IL-1β, IL-6 and TNF-α in serum within 8 weeks (P < 0.05), and its anti-inflammatory effect is similar to oral celecoxib (negative control group). Puerarin has a certain protective effect on articular cartilage and can reduce the level of biomarkers CTX-II, COMP and CS 846 in serum and synovial fluid in the early stage of OA (P < 0.05). In addition, the clinical scores and radiographs scores were significantly reduced after 8 weeks of puerarin treatment (P < 0.05). ConclusionsCanine OA cartilage may be mediated through anti-inflammatory, anti-metabolism and anabolic effects, and strongly down-regulate the inflammatory factors IL-1β, IL-6 and TNF-α and cartilage degradation biomarkers CTX-II, COMP and CS 846 are related, providing a good alternative therapy for OA.
Alternative Splicing Events and Subtype Analysis of Esophageal Cancer
Authors: Zheng Yuanyuan , Shen Zhibo , Fan Zhirui , Wang Wenbin , Geng Qishun , Kan Quancheng , Xue Wenhua , Zhao Jie 
Published: October 2020

Abstract
Aim: Alternative splicing (AS) has been widely demonstrated in the occurrence and progression of many cancers. Nevertheless, the involvement of cancer-associated splicing in the development of esophageal carcinoma (ESCA) is still ambiguous.Method: RNA-Seq data and the corresponding clinical information of the ESCA cohort was downloaded from The Cancer Genome Atlas database. The splicing percentage value was calculated using a Java application called SpliceSeq, and differently expressed AS (DEAS) events and their splicing network were further analyzed using bioinformatics methods. Kaplan–Meier, Cox regression, and unsupervised cluster analyses were used to assess the association between AS events and clinical characteristics of ESCA patients.Results: A total of 50,342 AS events were identified, of which 3,988 were DEAS events; 46 of these were associated with overall survival (OS) of ESCA patients, and the 5-year OS rate was 0.941. By constructing a network of AS events with survival-related splicing factors and variable-shear events associated with prognosis, the regulatory relationship was further predicted. Four clusters with different survival patterns were revealed using unsupervised cluster analysis.Conclusion: ESCA-associated AS events and splicing networks are of great value in deciphering the underlying mechanisms of AS in ESCA and providing clues for therapeutic goals for further validation.
WITHDRAWN: Green Building Performance Assessment in China Using a Cloud Model
Authors: Li Xiao-Juan , Wang Chen , Bora Shilpi , Samuel Bimenyimana 
Published: October 2020

Abstract
Background. The recent pursuit of energy conservation, emission reduction, and sustainable development are the demanding aspect in green building construction. Green building concept becomes important in the current building life cycle to mitigate environmental issues. In this way, a strategy for surveying the performance of a green structure is attractive. Consequently, this investigation intends to build up a methodical methodology for distinguishing the elements that decide the effective development and activity of a green structure and makes a technique for assessing green building execution.Methods. To accomplish these objectives, a comprehensive literature review was first conducted, followed by determining the performance of a green building to create a qualitative fuzzy set of performance metrics. A cloud model was used to analyze the factor hierarchy using weighted entropy to quantify the performance of a green building. Ultimately, an inside and out contextual analysis was led to check the exact discoveries.Results. A green building performance index that was created based on expert evaluations and weighted the indexes subjectively and objectively. Likewise, a standard cloud yardstick was also created in the comprehensive cloud. The sustainability of the building was qualified and assessed by comparing cloud images with the standard cloud. It has been analyzed that, the execution measurements and persuasive components make appraisal models for a green building. This investigation can help the development business to distinguish and manage issues that emerge in building green buildings. The coherent framework that the researchers have developed for assessing green buildings provides a solid foundation for further research into sustainability and green building operation. The result indicates that the industry acquires benefit by suggesting effective measures that can be implemented in all stages of green building construction.Conclusion. The analysis results demonstrated that this investigation builds up a novel exhibition appraisal of a green building which makes a target establishment for assessing building execution. Additionally, the findings are helpful for industry specialists to improve green building construction. Besides, the outcomes give significant data to governments and other invested individuals to create target models for green building development programs.
Amplified centrosomes in dendritic cells promote immune cell effector functions
Authors: Weier Ann-Kathrin , Homrich Mirka , Ebbinghaus Stephanie , Hauschild Robert , Quast Thomas , Mass Elvira , Schlitzer Andreas , Kolanus Waldemar , Burgdorf Sven , Kiermaier Eva 
Published: October 2020

Abstract
Centrosomes constitute structural elements organizing the mitotic spindle in animal cells for proper chromosome segregation. Centrosome numbers are tightly controlled and limited to one during interphase and two before a cell enters mitosis. Defects in regulating centrosome numbers lead to the presence of amplified centrosomes, which are a hallmark of malignant cells and sufficient to induce tumorigenesis. By contrast, amplified centrosomes are rarely observed in normal somatic cells and often removed during terminal differentiation. Here, we demonstrate the presence of amplified centrosomes in dendritic cells (DCs) during immune activation. Mature DCs accumulate centrosomes by mitotic defects and show high expression levels of polo-like kinase 2 (PLK2) leading to over-duplication of centrioles. During cell migration, amplified centrosomes tightly cluster and act as functional microtubule-organizing centers, which promote persistent locomotion. Moreover, DCs with amplified centrosomes show enhanced secretion of inflammatory cytokines and optimized T cell responses. Together, these results demonstrate a previously unappreciated role of amplified centrosomes in promoting the ability of leukocytes to enhance immune responses.
The prevention of penetration of unidentified screw of femoral neck fracture during the operation
Authors: Xu Xiaopei , Bai Jingqi , Liu Jinyuan , Wang Xiaohu , Lv Xin 
Published: October 2020

Abstract
BackgroundScrew internal fixation is one of the main surgical procedures for femoral neck fractures. Routine intraoperative fluoroscopy is hard to identify screw penetration, which becomes one of the important factors of postoperative hip pain and postoperative complications.MethodsCollect and analyze the intraoperative and postoperative imaging data of patients with internal fixation. Using geometric methods and analysis of anatomical characteristics, we explored the best imaging angle where the screw penetration was not found in the conventional 2D images of the anterior and lateral view during the operation, so that it can be determined whether there is screw penetration by taking a certain angle during the operation.ResultsThe unrecognized screw penetration rate during the operation was 25%, 5% penetrated from the back of the femoral head, and 20% penetrated the femoral neck part and then entered the head. The unrecognized screw of the femoral head is caused by the intersection of the anterior and lateral projection to form the Steinmetz solid. The study found that the special photographic orientation θ=90°—arctan (M1O′/M2O′). For the screw penetration of the femoral neck, the probability of occurrence in different areas of the femoral neck is 10.5% of the front superior part, 44.2% of the front inferior part, 28.6% of the back superior part, and 16.8% of the back inferior part. The best shooting directions of the front superior,front inferior, back superior, and back inferior through which the detection screw passes are the positive position ,35.8° to the tail side, 70° to the head side, 46.3° to the head side, and 40.5° to the tail side.ConclusionIt is important to avoid unrecognized screw penetration during the operation. In this study, it was concluded that a certain angle was taken during the operation to determine whether there was screw penetration, which significantly reduced the incidence of screw penetration of the femoral head and femoral neck.
A novel indicator of children lipid accumulation product associated with dyslipidemia in Chinese children and adolescents
Authors: Wang Bangxuan , Yuan Yongting , Hu Jingyao , Sun Lili , Yao Rongying , Han Hui , Ma Jun , Fu Lianguo 
Published: October 2020

Abstract
Background and aimsThe dyslipidemia contributed to more than half Cardiovascular disease (CVD) which ranked first in all causes of death in the world. Children’s lipid accumulation product (CLAP) is significantly related to cardiac metabolic risk factors in children and adolescents. The present study was to explore a novel indicator of children’s lipid accumulation product (CLAP) associated with dyslipidemia in Chinese children and adolescents.MethodsA total of 683 children and adolescents aged 8-15 years were recruited using the stratified cluster sampling method in this cross-sectional study, and were measured their body height, weight, waist circumference (WC), abdominal skinfold thickness (AST), triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C), dietary behaviors and physical activities. A logistic regression model and receiver operating characteristic curve (ROC curve) were used to compare the effects of CLAP for predicting dyslipidemia.ResultsThe prevalence of dyslipidemia was 13.6% (13.9% in boys and 13.2% in girls). The AUC (95%CI) of CLAP for predicting dyslipidemia was 0.76 (0.66-0.84) in girls, was 0.83 (0.76-0.89) in boys, and was higher than those of Sweight, SWC, SAST, SWHtR, and SBMI, respectively. The P85 of CLAP was the optimal value to predict dyslipidemia among girls (OR (95% CI): 10.54(5.09-21.82), AUC (95% CI): 0.72 (0.62-0.81). The P75 of CLAP was the optimal value to predict dyslipidemia among boys (OR (95% CI): 8.74(4.54-16.85), AUC (95% CI): 0.73 (95% CI 0.65-0.81).ConclusionsThe CLAP was a novel indicator associated with dyslipidemia in Chinese children and adolescents, and performed better than weight, WC, AST, WHtR, and BMI.
Atomic design and fine-tuning of sub-nanometric Pt catalysts to tame hydrogen generation
Authors: Yang Jie , Fu Wenzhao , Chen Chaoqiu , Chen Wenyao , Huang Wugen , Yang Ruoou , Kong Qing-Qiang , Zhang Baiyan , Zhao Jixiao , Chen Cheng-Meng , Luo Jun , Yang Fan , Duan Xuezhi , Jiang Zheng , Qin Yong 
Published: October 2020

Abstract
Rational synthesis of sub-nanocatalysts with controllable electronic and atomic structures remains a challenge to break the limits of traditional catalysts for superior performance. Here we report the atomic-level precise synthesis of Pt/graphene sub-nanocatalysts (from single atom, dimer, and to cluster) by atomic layer deposition, achieved by a novel high temperature pulsed ozone strategy to controllably pre-create abundant in-plane epoxy groups on graphene as anchoring sites. The specific in-plane epoxy structure endows the deposited Pt species with outstanding uniformity, controllability and stability. Their size-depended electronic and geometric effects have been observed for ammonia borane hydrolysis, revealing a volcano-type dependence of intrinsic activity on their sizes. Their active site structures have been identified based on extensive characterizations, dynamic compensation effect, kinetic isotope experiments and density function theory simulation. The Pt dimers show the highest catalytic activity and good durability than Pt single atoms and nanoparticles, ascribed to the unique C-Pt-Pt-O (C5Pt2O, metal-metal bond dimer) active site structure. Our work provides new insights into the precise tailoring and catalytic mechanism in sub-nanometer level.