bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical impact of primary tumour location, early tumour shrinkage, and depth of response in the treatment of metastatic colorectal cancer with first-line chemotherapy plus cetuximab or bevacizumab
Authors: Sagawa Tamotsu , Sato Yasushi , Hirakawa Masahiro , Hamaguchi Kyoko , Fukuya Akira , Okamoto Koichi , Miyamoto Hiroshi , Muguruma Naoki , Fujikawa Koshi , Takahashi Yasuo , Takayama Tetsuji 
Published: November 2020

AbstractThe primary tumour location is an important prognostic factor for previously untreated metastatic colorectal cancer (mCRC). However, the predictive efficacies of primary tumour location, early tumour shrinkage (ETS), and depth of response (DpR) on mCRC treatment has not been fully evaluated. This study aimed to investigate the predictive efficacies of these traits in mCRC patients treated with first-line 5-fluorouracil-based chemotherapy plus biologic agents, namely, cetuximab and bevacizumab. This was a retrospective analysis of the medical records of 110 patients with pathology-documented unresectable mCRC. Patients with left-sided mCRC receiving any first-line regimen showed better overall survival (OS) than those with right-sided mCRC [33.3 vs 16.3 months; hazard ratio (HR) 0.44; 95% confidence interval (CI) 0.27–0.74; p < 0.001]. In patients with left-sided tumours, treatment with chemotherapy plus cetuximab yielded longer OS than chemotherapy plus bevacizumab (50.6 vs 27.8 months, HR 0.55; 95% CI 0.32–0.97; p = 0.0378). mCRC patients with ETS and high DpR showed better OS than those lacking ETS and with low DpR (33.5 vs 19.6 months, HR 0.50, 95% CI 0.32–0.79, p = 0.023 and 38.3 vs 19.0 months, HR 0.43, 95% CI 0.28–0.68, p < 0.001, respectively). Moreover, ETS and/or high DpR achieved in patients with right-sided mCRC receiving chemotherapy plus cetuximab were associated with significantly better OS than in those lacking ETS and with low DpR (34.3 vs 10.4 months, HR 0.19, 95% CI 0.04–0.94, p = 0.025 and 34.3 vs 10.4 months, HR 0.19, 95% CI 0.04–0.94, p = 0.0257, respectively). Taken together, our study demonstrates that primary tumour location is not only a well-known prognostic factor but also a relevant predictive factor in patients with mCRC receiving chemotherapy plus cetuximab. Additionally, both ETS and DpR could predict treatment outcomes and also potentially guide cetuximab treatment even in right-sided mCRCs.
Exploring the construction and infiltration strategies of social bots in sina microblog
Authors: Wang Wenxian , Chen Xingshu , Jiang Shuyu , Wang Haizhou , Yin Mingyong , Wang Peiming 
Published: November 2020

AbstractNowadays, millions of people use Online Social Networks (OSNs) like Twitter, Facebook and Sina Microblog, to express opinions on current events. The widespread use of these OSNs has also led to the emergence of social bots. What is more, the existence of social bots is so powerful that some of them can turn into influential users. In this paper, we studied the automated construction technology and infiltration strategies of social bots in Sina Microblog, aiming at building friendly and influential social bots to resist malicious interpretations. Firstly, we studied the critical technology of Sina Microblog data collection, which indicates that the defense mechanism of that is vulnerable. Then, we constructed 96 social bots in Sina Microblog and researched the influence of different infiltration strategies, like different attribute settings and various types of interactions. Finally, our social bots gained 5546 followers in the 42-day infiltration period with a 100% survival rate. The results show that the infiltration strategies we proposed are effective and can help social bots escape detection of Sina Microblog defense mechanism as well. The study in this paper sounds an alarm for Sina Microblog defense mechanism and provides a valuable reference for social bots detection.
Cessation of Bezafibrate in patients with chronic kidney disease improves renal function
Authors: Zingerman Boris , Ziv Danny , Feder Krengel Netta , Korzets Asher , Matok Ilan 
Published: November 2020

AbstractBezafibrate (BzF) is eliminated by renal excretion and dosage must be reduced in patients with chronic kidney disease (CKD). There is a concern that BzF causes a further deterioration in renal function in patients with CKD. This study assessed whether BzF discontinuation or dose reduction in CKD patients improves renal function. 117 CKD patients treated with BzF between 2009 and 2014 were studied for demographics, comorbid conditions and laboratory variables. Data compared 2 groups: an intervention group of 64 patients where recommendations regarding BzF administration was implemented and a control group of 37 patients. Follow-up was maintained for 12 months. In the intervention group, estimated glomerular filtration rate (eGFR) increased from 38 to 42 mL/min/1.73 m2 (p = 0.01); blood urea levels decreased from 81 to 77 mg/dL (p = 0.04). Serum creatinine decreased by more than 0.2 mg/dL in 45% of the intervention group, as compared to 19% of the control group (p < 0.01). Improvement in eGFR was seen exclusively in patients who stopped BzF completely (eGFR increased from 38 to 44 mL/min/1.73 m2). In the intervention group, TG level increased from 183 to 220 mg/dL (p < 0.001). BzF cessation in approximately 50% of patients with CKD was associated with an increase in eGFR.
Characterisation of CD4+ T-cell subtypes using single cell RNA sequencing and the impact of cell number and sequencing depth
Authors: Ding James , Smith Samantha L. , Orozco Gisela , Barton Anne , Eyre Steve , Martin Paul 
Published: November 2020

AbstractCD4+ T-cells represent a heterogeneous collection of specialised sub-types and are a key cell type in the pathogenesis of many diseases due to their role in the adaptive immune system. By investigating CD4+ T-cells at the single cell level, using RNA sequencing (scRNA-seq), there is the potential to identify specific cell states driving disease or treatment response. However, the impact of sequencing depth and cell numbers, two important factors in scRNA-seq, has not been determined for a complex cell population such as CD4+ T-cells. We therefore generated a high depth, high cell number dataset to determine the effect of reduced sequencing depth and cell number on the ability to accurately identify CD4+ T-cell subtypes. Furthermore, we investigated T-cell signatures under resting and stimulated conditions to assess cluster specific effects of stimulation. We found that firstly, cell number has a much more profound effect than sequencing depth on the ability to classify cells; secondly, this effect is greater when cells are unstimulated and finally, resting and stimulated samples can be combined to leverage additional power whilst still allowing differences between samples to be observed. While based on one individual, these results could inform future scRNA-seq studies to ensure the most efficient experimental design.
Palaeo-productivity record from Norwegian Sea enables North Atlantic Oscillation (NAO) reconstruction for the last 8000 years
Authors: Becker Lukas W. M. , Sejrup Hans Petter , Hjelstuen Berit O. , Haflidason Haflidi , Kjennbakken Heidi , Werner Johannes P. 
Published: November 2020

AbstractThe North Atlantic Oscillation is the dominant atmospheric driver of North Atlantic climate variability with phases corresponding to droughts and cold spells in Europe. Here, we exploit a suggested anti-correlation of North Atlantic Oscillation-phase and north-eastern North Atlantic primary productivity by investigation of south-eastern Norwegian Sea sediment cores spanning the last 8000 years. Age model uncertainties between 2 and 13 years for the period 1992–1850 AD allows for the proxy to observational data calibration. Our data suggest that Ca/Fe core-scanning results reflect sedimentary CaCO3variability in the region. Cross-correlating the Ca/Fe record with nearby phytoplankton counts and dissolved O2data suggests that Ca/Fe can be used as a proxy for primary productivity variability in the region. Our data support an anti-correlation of primary productivity to the winter North Atlantic Oscillation index. Hence, we propose a sub-decadally resolved palaeo-North Atlantic Oscillation reconstruction based on an open-ocean record spanning the last 8000 years.
On-treatment measurements of circulating tumor DNA during FOLFOX therapy in patients with colorectal cancer
Authors: Moser Tina , Waldispuehl-Geigl Julie , Belic Jelena , Weber Sabrina , Zhou Qing , Hasenleithner Samantha O. , Graf Ricarda , Terzic Jasmin Alia , Posch Florian , Sill Heinz , Lax Sigurd , Kashofer Karl , Hoefler Gerald , Schoellnast Helmut , Heitzer Ellen , Geigl Jochen B. , Bauernhofer Thomas , Speicher Michael R. 
Published: November 2020

AbstractWe addressed a significant unknown feature of circulating tumor DNA (ctDNA), i.e., how ctDNA levels change during chemotherapy, by serially monitoring ctDNA in patients with colorectal cancer during the 48-h application of FOLFOX. Surprisingly, we did not observe a spike in ctDNA as a sign of a responsive tumor, but instead ctDNA levels initially decreased and remained low in patients with stable disease or partial response. Our observations reveal further insights into cell destruction during chemotherapy with important implications for the management of patients.
Let Sleeping Patients Lie, avoiding unnecessary overnight vitals monitoring using a clinically based deep-learning model
Authors: Tóth Viktor , Meytlis Marsha , Barnaby Douglas P. , Bock Kevin R. , Oppenheim Michael I. , Al-Abed Yousef , McGinn Thomas , Davidson Karina W. , Becker Lance B. , Hirsch Jamie S. , Zanos Theodoros P. 
Published: November 2020

AbstractImpaired sleep for hospital patients is an all too common reality. Sleep disruptions due to unnecessary overnight vital sign monitoring are associated with delirium, cognitive impairment, weakened immunity, hypertension, increased stress, and mortality. It is also one of the most common complaints of hospital patients while imposing additional burdens on healthcare providers. Previous efforts to forgo overnight vital sign measurements and improve patient sleep used providers’ subjective stability assessment or utilized an expanded, thus harder to retrieve, set of vitals and laboratory results to predict overnight clinical risk. Here, we present a model that incorporates past values of a small set of vital signs and predicts overnight stability for any given patient-night. Using data obtained from a multi-hospital health system between 2012 and 2019, a recurrent deep neural network was trained and evaluated using ~2.3 million admissions and 26 million vital sign assessments. The algorithm is agnostic to patient location, condition, and demographics, and relies only on sequences of five vital sign measurements, a calculated Modified Early Warning Score, and patient age. We achieved an area under the receiver operating characteristic curve of 0.966 (95% confidence interval [CI] 0.956–0.967) on the retrospective testing set, and 0.971 (95% CI 0.965–0.974) on the prospective set to predict overnight patient stability. The model enables safe avoidance of overnight monitoring for ~50% of patient-nights, while only misclassifying 2 out of 10,000 patient-nights as stable. Our approach is straightforward to deploy, only requires regularly obtained vital signs, and delivers easily actionable clinical predictions for a peaceful sleep in hospitals.
ppGpp functions as an alarmone in metazoa
Authors: Ito Doshun , Kawamura Hinata , Oikawa Akira , Ihara Yuta , Shibata Toshio , Nakamura Nobuhiro , Asano Tsunaki , Kawabata Shun-Ichiro , Suzuki Takashi , Masuda Shinji 
Published: November 2020

AbstractGuanosine 3′,5′-bis(pyrophosphate) (ppGpp) functions as a second messenger in bacteria to adjust their physiology in response to environmental changes. In recent years, the ppGpp-specific hydrolase, metazoan SpoT homolog-1 (Mesh1), was shown to have important roles for growth under nutrient deficiency in Drosophila melanogaster. Curiously, however, ppGpp has never been detected in animal cells, and therefore the physiological relevance of this molecule, if any, in metazoans has not been established. Here, we report the detection of ppGpp in Drosophila and human cells and demonstrate that ppGpp accumulation induces metabolic changes, cell death, and eventually lethality in Drosophila. Our results provide the evidence of the existence and function of the ppGpp-dependent stringent response in animals.
HACS1 signaling adaptor protein recognizes a motif in the paired immunoglobulin receptor B cytoplasmic domain
Authors: Kwan Jamie J. , Slavkovic Sladjana , Piazza Michael , Wang Dingyan , Dieckmann Thorsten , Johnson Philip E. , Wen Xiao-Yan , Donaldson Logan W. 
Published: November 2020

AbstractHematopoietic adaptor containing SH3 and SAM domains-1 (HACS1) is a signaling protein with two juxtaposed protein–protein interaction domains and an intrinsically unstructured region that spans half the sequence. Here, we describe the interaction between the HACS1 SH3 domain and a sequence near the third immunoreceptor tyrosine-based inhibition motif (ITIM3) of the paired immunoglobulin receptor B (PIRB). From surface plasmon resonance binding assays using a mouse and human PIRB ITIM3 phosphopeptides as ligands, the HACS1 SH3 domain and SHP2 N-terminal SH2 domain demonstrated comparable affinities in the micromolar range. Since the PIRB ITIM3 sequence represents an atypical ligand for an SH3 domain, we determined the NMR structure of the HACS1 SH3 domain and performed a chemical shift mapping study. This study showed that the binding site on the HACS1 SH3 domain for PIRB shares many of the same amino acids found in a canonical binding cleft normally associated with polyproline ligands. Molecular modeling suggests that the respective binding sites in PIRB ITIM3 for the HACS1 SH3 domain and the SHP2 SH2 domain are too close to permit simultaneous binding. As a result, the HACS1-PIRB partnership has the potential to amalgamate signaling pathways that influence both immune and neuronal cell fate.
Charge guides pathway selection in β-sheet fibrillizing peptide co-assembly
Authors: Seroski Dillon T. , Dong Xin , Wong Kong M. , Liu Renjie , Shao Qing , Paravastu Anant K. , Hall Carol K. , Hudalla Gregory A. 
Published: November 2020

AbstractPeptide co-assembly is attractive for creating biomaterials with new forms and functions. Emergence of these properties depends on the peptide content of the final assembled structure, which is difficult to predict in multicomponent systems. Here using experiments and simulations we show that charge governs content by affecting propensity for self- and co-association in binary CATCH(+/−) peptide systems. Equimolar mixtures of CATCH(2+/2−), CATCH(4+/4−), and CATCH(6+/6−) formed two-component β-sheets. Solid-state NMR suggested the cationic peptide predominated in the final assemblies. The cationic-to-anionic peptide ratio decreased with increasing charge. CATCH(2+) formed β-sheets when alone, whereas the other peptides remained unassembled. Fibrillization rate increased with peptide charge. The zwitterionic CATCH parent peptide, “Q11”, assembled slowly and only at decreased simulation temperature. These results demonstrate that increasing charge draws complementary peptides together faster, favoring co-assembly, while like-charged molecules repel. We foresee these insights enabling development of co-assembled peptide biomaterials with defined content and predictable properties.
A simple hydrogel device with flow-through channels to maintain dissipative non-equilibrium phenomena
Authors: Dúzs Brigitta , Szalai István 
Published: November 2020

AbstractThe development of autonomous chemical systems that could imitate the properties of living matter, is a challenging problem at the meeting point of materials science and nonequilibrium chemistry. Here we design a multi-channel gel reactor in which out-of-equilibrium conditions are maintained by antagonistic chemical gradients. Our device is a rectangular hydrogel with two or more channels for the flows of separated reactants, which diffuse into the gel to react. The relative position of the channels acts as geometric control parameters, while the concentrations of the chemicals in the channels and the variable composition of the hydrogel, which affects the diffusivity of the chemicals, can be used as chemical control parameters. This flexibility allows finding easily the optimal conditions for the development of nonequilibrium phenomena. We demonstrate this straightforward operation by generating diverse spatiotemporal patterns in different chemical reactions. The use of additional channels can create interacting reaction zones.
Emergence of quasiparticles in a doped Mott insulator
Authors: Wang Yao , He Yu , Wohlfeld Krzysztof , Hashimoto Makoto , Huang Edwin W. , Lu Donghui , Mo Sung-Kwan , Komiya Seiki , Jia Chunjing , Moritz Brian , Shen Zhi-Xun , Devereaux Thomas P. 
Published: November 2020

AbstractHow a Mott insulator develops into a weakly coupled metal upon doping is a central question to understanding various emergent correlated phenomena. To analyze this evolution and its connection to the high-Tc cuprates, we study the single-particle spectrum for the doped Hubbard model using cluster perturbation theory on superclusters. Starting from extremely low doping, we identify a heavily renormalized quasiparticle dispersion that immediately develops across the Fermi level, and a weakening polaronic side band at higher binding energy. The quasiparticle spectral weight roughly grows at twice the rate of doping in the low doping regime, but this rate is halved at optimal doping. In the heavily doped regime, we find both strong electron-hole asymmetry and a persistent presence of Mott spectral features. Finally, we discuss the applicability of the single-band Hubbard model to describe the evolution of nodal spectra measured by angle-resolved photoemission spectroscopy (ARPES) on the single-layer cuprate La2−xSrxCuO4 (0 ≤ x ≤ 0.15). This work benchmarks the predictive power of the Hubbard model for electronic properties of high-Tc cuprates.
Optical wave-packet with nearly-programmable group velocities
Authors: Li Zhaoyang , Kawanaka Junji 
Published: November 2020

AbstractDuring the process of Bessel beam generation in free space, spatiotemporal optical wave-packets with tunable group velocities and accelerations can be created by deforming pulse-fronts of injected pulsed beams. So far, only one determined motion form (superluminal or luminal or subluminal for the case of group velocity; and accelerating or uniform-motion or decelerating for the case of acceleration) could be achieved in a single propagation path. Here we show that deformed pulse-fronts with well-designed axisymmetric distributions (unlike conical and spherical pulse-fronts used in previous studies) allow us to obtain nearly-programmable group velocities with several different motion forms in a single propagation path. Our simulation shows that this unusual optical wave-packet can propagate at alternating superluminal and subluminal group velocities along a straight-line trajectory with corresponding instantaneous accelerations that vary periodically between positive (acceleration) and negative (deceleration) values, almost encompassing all motion forms of the group velocity in a single propagation path. Such unusual optical wave-packets with nearly-programmable group velocities may offer new opportunities for optical and physical applications.
Active-matrix monolithic gas sensor array based on MoS2 thin-film transistors
Authors: Kim Sehwan , Park Heekyeong , Choo Sooho , Baek Seongho , Kwon Yena , Liu Na , Yang Jeong Yong , Yang Cheol-Woong , Yoo Geonwook , Kim Sunkook 
Published: November 2020

AbstractHighly sensitive and system integrable gas sensors play a significant role in industry and daily life, and MoS2 has emerged as one of the most promising two-dimensional nanomaterials for gas sensor technology. In this study, we demonstrate a scalable and monolithically integrated active-matrix gas sensor array based on large-area bilayer MoS2 films synthesized via two-successive steps: radio-frequency magnetron sputtering and thermal sulfurization. The fabricated thin-film transistors exhibit consistent electrical performance over a few centimeters area and resulting gas sensors detect NO2 with ultra-high sensitivity across a wide detection range, from 1 to 256 ppm. This is due to the abundant grain boundaries of the sputtered MoS2 channel, which perform as active sites for absorption of NO2 gas molecules. The demonstrated active-matrix gas sensor arrays display good switching capabilities and are anticipated to be readily integrated with additional circuitry for different gas sensing and monitoring applications.
Taking Language Samples Home: Feasibility, Reliability, and Validity of Child Language Samples Conducted Remotely With Video Chat Versus In-Person
Authors: Manning Brittany L. , Harpole Alexandra , Harriott Emily M. , Postolowicz Kamila , Norton Elizabeth S. 
Published: November 2020

Purpose
There has been increased interest in using telepractice for involving more diverse children in research and clinical services, as well as when in-person assessment is challenging, such as during COVID-19. Little is known, however, about the feasibility, reliability, and validity of language samples when conducted via telepractice.

Method
Child language samples from parent–child play were recorded either in person in the laboratory or via video chat at home, using parents' preferred commercially available software on their own device. Samples were transcribed and analyzed using Systematic Analysis of Language Transcripts software. Analyses compared measures between-subjects for 46 dyads who completed video chat language samples versus 16 who completed in-person samples; within-subjects analyses were conducted for a subset of 13 dyads who completed both types. Groups did not differ significantly on child age, sex, or socioeconomic status.

Results
The number of usable samples and percent of utterances with intelligible audio signal did not differ significantly for in-person versus video chat language samples. Child speech and language characteristics (including mean length of utterance, type–token ratio, number of different words, grammatical errors/omissions, and child speech intelligibility) did not differ significantly between in-person and video chat methods. This was the case for between-group analyses and within-child comparisons. Furthermore, transcription reliability (conducted on a subset of samples) was high and did not differ between in-person and video chat methods.

Conclusions
This study demonstrates that child language samples collected via video chat are largely comparable to in-person samples in terms of key speech and language measures. Best practices for maximizing data quality for using video chat language samples are provided.