bluuebunny/crossref_metadata_2025_split huggingface dataset:
HUBUNGAN PERILAKU PERSONAL HYGIENE DAN STATUS KECACINGAN DENGAN STATUS GIZI ANAK SEKOLAH DASAR DI WILAYAH KERJA PUSKESMAS II SUMBANG
Authors: Arifin Lida , Purnamasari Dyah Umiyarni 
Published: April 2020

Background : Nutrition is one kind of important factors that build qualified human resources. A child being underweight due to two important things, they are food intake and illness. One type of infection that is often found in children that worm infection. The infection is often found in developing countries with lack of personal hygiene. The purpose of this research is to determine relation between personal hygiene and status worm infection with nutritional status among primary children.

Methods : This study design was observational analytic study with cross sectional approach. The sample were 51 children fourth grade 01 Banjarsari Kulon and 02 Banjarsari Wetan Primary School.

Results : Respondents with normal nutritional status 72%. Respondents with good behavior personal hygiene 59%. Respondents with positive helminthiasis status 53%. Relation between personal hygiene and nutritional status was examined by chi square (p value 1.000 > 0.05). Relation between helminthiasis status and nutritional status p value 0.425. Relation between personal hygiene and helminthiasis status p value 0.625.

Conclusion : There is no relation between the behavior of personal hygiene and nutritional status of school children in Puskesmas II Sumbang. There is no relation between helminthiasis status and nutritional status of school children in Puskesmas II Sumbang. There is no relation between personal hygiene and helminthiasis status.
SUBSTITUSI TEPUNG AMPAS KELAPA DALAM PEMBUATAN BROWNIES KUKUS TERHADAP SIFAT ORGANOLEPTIK DAN NILAI GIZI
Authors: Septiani Septiani , Hiyanah Nur 
Published: April 2020

The utilization of coconut pulp flour in the food product making is still very limited. This research to analyze brownies that are distuited coconut pulp flour against nutritional value. This research is an experimental research with level of substitution of wheat flour with coconut pulp flour as treatment. There are 3 treatments, that is F1 (55% wheat flour and 45% coconut pulp flour), F2 (45% wheat flour and 55% coconut pulp flour) ad F3 (35% wheat flour and 65% coconut pulp flour). Formula results selected from the organoleptic test who is F1 with comparison 55:45 continued with result proximate test that obtained water rate, ash rate, fat, protein, carbohydrate and fiber in way continue that 23,88%, 1,91%, 7,39%, 5,78%, 61,04 and 1,82%. The conclusion of this research is that have differences (p <0.05) between the formula result to water rate, ash rate, protein, carbohydrate and fiber.
The Effect of Addition of Ginger Extract and Kencur Extract on the Physicochemical Properties of Instant Temulawak and the Sensory Properties of the Beverage
Authors: Septiana Aisyah Tri 
Published: April 2020

AbstrACT
Temulawak has health benefits, but it is sensationally less favored because it is bitter and has an unpleasant odor, so that in its processing often other spices such as ginger and kencur are added. Temulawak can be processed into temulawak instant drink. This study aims to determine the effect of the concentration of ginger extract (0%, 15%, 30%) and kencur (0%, 15%, 30%) on the physicochemical properties of temulawak instant and sensory brewed drinks. Physicochemical measurements were carried out by analyzing the water content and density of cages of temulawak instant drink. Sensory analysis included color, aroma, spicy taste, sweetness, flavor and likeness. The results showed that the addition of ginger and kencur extract tended to increase water content. The addition of ginger extract and kencur up to 15% increase the flavor and preference of ginger drink. The best combination of the addition of ginger extract and kencur extract was obtained in the treatment of adding ginger extract 15% and the concentration of galingale extract 15% with physicochemical properties of powder drink: water content of 5.20% bb and kamba density of 0.608 g / ml. color 3.24 (brownish yellow); 2.68 spicy flavor (approaching somewhat spicy); sweetness level 3,4 (somewhat sweet); flavor 3.51 (close to tasty); fondness 3.43 (few likes).
A Tangent-Line Approach for Effective Density Used in the Ideal Mixing Rule: Part II—Evaluation of Mixing Characteristics of Oil/Gas Systems and Application Criteria
Authors: Chen Zehua , Yang Daoyong (Tony) 
Published: April 2020

Summary
Although in Part I of this study (Chen and Yang 2020) we developed a tangent-line approach for effective density that is more general, robust, and flexible than the methods proposed by Saryazdi (2012) and Saryazdi et al. (2013), its application is only limited to heavy-oil/bitumen-associated mixtures [i.e., specifically, it has only been applied to bitumen-rich liquid phase (denoted as L2)]. As indicated in Part I, the density of nitrogen (N2)/hydrocarbon mixtures cannot be accurately predicted by using the ideal mixing rule (IM) with either real density or effective density. Not only do we need to explain and evaluate the observed deviations and patterns, but also the density prediction of solvent/Fraction 1 systems [i.e., Fraction 1 of the Athabasca bitumen, which has a molecular weight (MW) of 268.8 g/mol, as reported in Azinfar et al. (2018a, 2018b, 2018c)] needs to be improved for practical use. In this study, we evaluate the mixing characteristics of different molecules in a mixture using the tangent-line approach. By evaluating and comparing performances of the IM with effective density (IM-E) and the IM with real density (IM-R), the observed patterns and deviations together with those calculated from the Westman equation indicate that the oil/gas molecules somewhat behave like solid particles in mixing. Accordingly, we further modify the effective density used in the IM to bridge the gap between the IM-E and the IM-R. The database has been extended to light-oil/gas systems such as black oils, volatile oils, gas condensates, carbon dioxide (CO2) miscible fluids, sour gases, and wet/dry gases. The IM with modified effective density (IM-ME) has also been applied to solvent/Fraction 1 systems and the C2 or C3 or n-C4-extraction L1 phase (bitumen-related mixtures) with better accuracy. Also, we develop new criteria for the uses of the IM-E, IM-ME, and IM-R that can cover the density predictions for almost all types of oil/gas systems in the petroleum industry with high accuracy. The performances of the IM are thoroughly evaluated and compared with the volume-translated (VT) Peng-Robinson equation of state (EOS) (VT PR EOS), from which the deviations provide new insights for accurately quantifying the mixture density in a more robust and reliable manner.
Successful pregnancy after complete resection of leiomyomatosis peritonealis disseminate without recurrence:A case report with next generation sequencing analysis and literature review
Authors: Bu Hualei , Jin Chengjuan , Fang Yan , Ma Yana , Wang Xiao , Chen Jingying , Chen Lijun 
Published: April 2020

Abstract
Background: Peritoneal leiomyomatosis disseminate (LPD) is a rare disease characterized by widespread dissemination of leiomyomas nodules throughout the peritoneal and omental surfaces. Reports of pregnancy with LPD are even rarer. Therefore, there is no clear consensus on the treatment of LPD on pregnancy, and the pathogenesis is still unclear. Case presentation：We reported a case of LPD patient who developed during pregnancy. The patient underwent cesarean section at 32 weeks of gestation while removing all visible tumors, and no LPD lesions were seen in the subsequent cesarean section at full-term. NGS of LPD lesions detected 4 mutations with focal high-level amplifications of CDK4 (Cyclin-dependent kinases 4), NBN (Nibrin), DAXX (Death domain associated protein) and MYC (Myelocytomatosis oncogene). Immunohistochemistry staining analysis among benign leiomyoma, LPD and leiomyosarcoma verified that LPD was an unusual intermediate between benign and malignant uterine smooth muscle tumors. Besides, LPD is a hormonal-dependent leiomyoma. After detailed literature search, we summarized the detailed clinical features and follow-up information of patients with LPD during pregnancy.Conclusions: This is the first reported LPD case of successful term pregnancy without recurrence, following resection of all visible lesions in prior pregnancy. LPD is an unusual intermediate between benign and malignant uterine smooth muscle tumors.
Kethoxal-assisted single-stranded DNA sequencing (KAS-seq) for capturing transcription dynamics and enhancer activity
Authors: He Chuan , Wu Tong , Lyu Ruitu 
Published: April 2020

Abstract

We describe a rapid and sensitive labeling of single-stranded DNA for sequencing (KAS-seq), based on a kethoxal-guanine reaction. KAS-seq procedure involves N
3
-kethoxal labeling, DNA isolation, biotinylation, fragmentation and enrichment, library preparation and sequencing. The whole process can be finished in a day. KAS-seq enables rapid measurement of the dynamics of transcriptionally-engaged Pol II, transcribing enhancers, Pol I and Pol III activities, and non-canonical DNA structures involving ssDNA simultaneously
in situ
, by using as few as 1,000 cells or mice tissues. The robust and tissue-friendly nature coupled with low input material requirement make KAS-seq a method that can be broadly applied. This step-by-step protocol is related to the publication “Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity
in situ
”

in
Nature Methods
.
A protocol for evaluating a multi-level implementation theory to scale up obstetric triage in referral hospitals in Ghana
Authors: Williams Caitlin Rain , Bogdewic Stephanie , Owen Medge , Srofe Emmanuel K. , Ramaswamy Rohit 
Published: April 2020

Abstract
Background: Ghana significantly reduced maternal and newborn mortality between 1990 and 2015, largely through efforts focused on improving access to care. Yet achieving further progress requires improving the quality and timeliness of care. Beginning in 2013, Ghana Health Service and Kybele, a US-based non-governmental organization, developed an innovative obstetric triage system to help midwives assess, diagnosis, and determine appropriate care plans more quickly and accurately. In 2019, efforts began to scale this successful intervention into six additional hospitals. This protocol describes the theory-based implementation approach guiding scale-up and presents the proposed mixed-methods evaluation plan. Methods: An implementation theory was developed to describe how complementary implementation strategies would be bundled into a multi-level implementation approach. Drawing on the Interactive Systems Framework and Evidenced Based System for Implementation Support, the proposed implementation approach is designed to help individual facilities develop implementation capacity and also build a learning network across facilities to support the implementation of evidence-based interventions.A convergent design mixed methods approach will be used to evaluate implementation with relevant data drawn from tailored assessments, routinely collected process and quality monitoring data, textual analysis of relevant documents and WhatsApp group messages, and key informant interviews. Implementation outcomes of interest are acceptability, adoption, and sustainability.Discussion: The past decade has seen a rapid growth in the development of frameworks, models, and theories of implementation, yet there remains little guidance on how to use these to operationalize implementation practice. This study proposes one method for using implementation theory, paired with other kinds of mid-level and program theory, to guide the replication and evaluation of a clinical intervention in a complex, real-world setting. The results of this study should help to provide evidence of how implementation theory can be used to help close the “know-do” gap.
WITHDRAWN: Rumen fermentation kinetics as impacted by red amaranth (Amaranthus cruentus, L) leaf powder using in vitro gas production technique
Authors: Ampapon Thiwakorn , Viennasay Bounnaxay , Wanapat Metha 
Published: April 2020

Abstract
Background
A need for research searching for alternative rumen enhancers warrants immediate attention. The in vitro fermentation experiment was conducted using factorial arrangement of two factors of roughage to concentrate and seven level of red amaranth leaf powder percentage of total substrate in a Completely randomized design (CRD). Two factors, namely Factor A was two ratio of roughage (R) to concentrate (C) at 60:40 and 40:60 and Factor B was level of red amaranth (Amaranthus cruentus, L) leaf powder (RALP) supplementation at 0, 2, 4, 6, 8, 10, and 12% of total dietary substrate.
Results
Red amaranth leaf powder (RALP) contained phytonutrients both condensed tannins and saponins in addition with high macro minerals (Ca, K, and Mg). This experiment revealed innovations of the RALP supplementation by enhancing rumen propionate (C3) production, reducing acetate (C2) to (C3) ratio, reducing protozoal population and mitigating methane (CH4) production. Furthermore, rumen dry matter degradation percentages were remarkably enhanced (P < 0.001) by increasing RALP supplementation.
Conclusion
Plants rich in phytonutrients and minerals such as red amaranth leaf powder (RALP) have a vital and promising role in modulating rumen fermentation, mitigating methane production, as well as increasing substrate degradability.
Antidyskinetic Treatment with the mGluR5 Antagonist MPEP Affects CaMKII/CREB/BDNF Molecular Pathways in the Parkinsonian Striatum
Authors: Huang Yixian , Zhang Qilin , zhan zhoubing , Shu Haiyang , Zhao Junyan , Zhen Tili , Luo Weifeng 
Published: April 2020

Abstract
Background

L-DOPA is still the gold-standard drug for the treatment of Parkinson's disease (PD). However, the long-term therapy often causes L-DOPA-induced dyskinesia (LID). Metabotropic glutamate receptor type 5 (mGluR5) is abundant in the basal ganglia, and its antagonists is thought to alleviates LID, but the underlying mechanisms have remained unclear.
Methods

We used 6-hydroxydopamine-lesioned rats to create PD rat model, PD rats were daily treated with L-DOPA alone or with MPEP 30 min before L-DOPA for 3 weeks, and at least 21 days of L-DOPA was administrated followed with microinjection of saline, CaMKII antagonist KN-93, anti-CREB, or anti-BDNF into the lesioned striatum of all the PD rats. The behavioral evaluation of abnormal involuntary movements(AIM) and rotational behavior tests were performed on the 2, 9, 11, 18, 21 and 23 days after drug application, and to tested the protein level of mGluR5, CaMKII, CREB and BDNF by western blot.
Results

Our results showed that MPEP cotreatment attenuates the abnormal involuntary movements, reversed the reduction of rotational response duration, and reduced overexpression of striatal mGluR5 and CaMKII/CREB/BDNF in the LID rats. Furthermore, we found that the CaMKII inhibitor KN-93, anti-CREB and anti-BDNF intrastriatal injection partly attenuates LID; KN-93 downregulated the striatal p-CaMKII, p-CREB and BDNF expression of the lesioned side, but the striatal mGluR5 expression without inhibition; anti-CREB downregulated the striatal p-CREB and BDNF expression of the lesioned side, but the striatal mGluR5 and p-CaMKII expression without inhibition; anti-BDNF downregulated the striatal BDNF expression of the lesioned side, but the mGluR5, CaMKII and CREB protein without inhibition.
Conclusions

These fundings suggested that mGluR5 specific antagonist MPEP attenuates L-DOPA-induced dyskinesia through affects CaMKII/CREB/BDNF molecular pathways in the striatum in this LID rat model.
Effect combined learning on oral health self-efficacy and self-care behaviors of students: a randomized controlled trial
Authors: Hashemi Zahra Sadat , khorsandi Mahboobeh , Shamsi Mohsen , Moradzadeh Rahmatollah 
Published: April 2020

Abstract
Background: In order to prevent oral diseases, the use of appropriate educational methods at childhood is one of the most important determinants of the public health. Therefore, the aim of this study was to investigate the effect of training through animations and games on oral health self-efficacy and self-care behaviors in students aged 6-12 years old. Methods: In this interventional study, 82 students were selected using cluster random sampling (38 subjects in the case group and 44 in the control group). The case group received 4 sessions of blended learning per week including animations and games while the control group received routine school education. Data were collected in six domains, including demographics, self-care, knowledge, attitude, behavior and self-efficacy before and 5 months after the intervention using a questionnaire. SPSS version 20 was used for data analysis. Results: Five months after the intervention, the mean score of self-care increased from 3.8 to 4.8 of 5, the mean score of self-efficacy increased from 36.8 to 48.9 of 70, and the mean score of behavior increased from 17.07 to 18.29 of 32, indicating significant changes (p < 0.05). There were no significant changes in these variables in the control group (p >0.05) Conclusion: Use of combined methods for oral health self-care education has positive effects on the students' performance and self-efficacy. IRCT registration number: This trial has been registered at IRCT. IRCT2017042133565N1 Registration date: 2017-05-17 https://en.irct.ir/trial/25851
Exploration on the clinical effect of different autotransfusion methods on patients with femoral shaft fracture surgery
Authors: Wang Huan , Yin Dan , Wang Jin-huo , Li Zhen-zhou , Xu Jia-Ming , Zhang Zi-Wei , Wang Xiao-Xiao , Kang Yi-Qun , Guo Jian-rong 
Published: April 2020

Abstract
BackgroundTo explore the clinical effect of predeposit, salvage and hemodilution autotransfusion on patients with femoral shaft fractdure (FSF) surgery.MethodsSelected FSF patients were randomly divided into 3 groups: intraoperative blood salvage autotransfusion group, preoperative hemodilution autohemotransfusion group and predeposit autotransfusion. After the operation for 5 days, body temperature, heart rate, blood platelet (PLT) and hemoglobin (Hb) of patients were determined; the concentrations of EPO and G-MSF in the 3 groups were calculated by ELISA assay; the content of CD14 + monocytes were calculated by FCM assay. The growth time and condition of the patient’s callus were determined at the 30th, 45th and 60th days after operation. Cox regression analysis was used to analyze the correlation between EP0, G-MSF, CD14 + mononuclear content, callus growth and autotransfusion methods.ResultsThere were no statistically significant differences in body temperature and heart rate between the 3 groups (P > 0.05). PLT and Hb in Predeposit group were markedly increased compared to that in Salvage group and Hemodilution group. The concentrations of EPO and G-MSF in Predeposit group were markedly increased compared to that in Salvage group and Hemodilution group. The content of CD14 + monocytes in Predeposit group was significantly higher compared to that in Salvage group and Hemodilution group. Predeposit autotransfusion promotes callus growth more quickly. The concentration of EP0 and G-MSF were positively correlated with callus growth in the three groups, and the content of CD14 + monocytes was not correlated in any of the three groups.ConclusionPredeposit autotransfusion promoted the recovery of FSF patients after operation more quickly compared to salvage autotransfusion and hemodilution autotransfusion.# These authors contributed equally to this work.
A study of the correlation between chalazion and intestinal flora in chinese children
Authors: Cui Yuanyue , Song Lei , Li Xie , Qiu Ting , Jin Jing , Zhao Keke , Zhu Xiaowei , Qu Jifang , Cao Qing , Jiang Bo 
Published: April 2020

Abstract
BackgroundChalazion is a chronic inflammatory granuloma of the meibomian gland formed on the basis of the obstruction of the meibomian gland drainage duct and the retention of secretions. It is one of the most common clinically eye diseases in children. Chronic inflammation of the meibomian glands is responsible for this disease, and the gut flora is thought to be involved in the inflammatory process. In this study, we investigated the relationship between intestinal microbial composition and children's chalazion.MethodsFecal samples were collected from 21 children with chalazion and 26 healthy children. DNA was extracted from fecal stool samples and 16S rRNA sequences in the gut flora were detected by using second second-generation sequencing technology. The results were used to compare the composition of the microbiome between patients and healthy controls.ResultsAccording to Alpha Diversity and Beta Diversity analysis, we found that there was no significant difference in bacteria diversity and relative abundance between the two groups. We compared the flora of the control group and the diseased group through Lefse analysis, and screened out 11 different species. Based on the absolute abundance of species, 43 different species were selected. Anosim analysis and metastats analysis were used to compare the flora of the control group and the diseased group again. At the species level, we found that gut_metagenome and human_gut_metagenome are the common differences in species levels obtained from the above analysis. Finally, corrplot correlation analysis was performed, suggesting that gut_metagenome has a great correlation with the number, ulceration, and recurrence of chalazion in children.ConclusionsThere was no significant difference in the diversity index and relative abundance of flora in children with chalazion compared with healthy children, but there were significant differences in some bacterial species. The gut_metagenome strains identified in this study were significantly related to the growth, ulceration, and relapse of children with chalazion. It is suggested that gut_metagenome may be a microbiological indicator which is independent of clinicopathologic factors but associated with chalazion disease .* These authors have contributed equally to this work.
Characteristics of acute pancreatitis with diabetic ketoacidosis and analysis of factors related to the length of hospital stay
Authors: Dong Fengliang , He Xiaoyan , Yang Qunying , Lou Jiangming , Wang Bei 
Published: April 2020

Abstract
Obejective 11% of adults with diabetic ketoacidosis (DKA) also suffer from AP. The obejective of this research was to study the characteristics of AP with DKA, the factors related to the length of hospital stay, and to explore indicators for the severity of AP.Methods A retrospective analysis of 173 AP with DKA cases treated in our hospital from July 2011 to December 2020 was performed. We analyzed the gender, age, systemic inflammatory response syndrome(SIRS), pleural effusion, blood urea nitrogen（BUN）, bedside index for the severity in acute pancreatitis（BISAP）score, white blood cell（WBC）, aspartate transaminase （AST）, lactate dehydrogenase（LDH）, blood glucose, Ranson score, partial pressure of carbon dioxide （PaCO 2 ）,triglyceride, modified CT severity index （MCTSI）, potential of hydrogen potential of hydrogen （PH）, amylase, glycated hemoglobin, C-reactive protein（CRP）, creatinine（Cr）, blood calcium, procalcitonin （PCT）, the length of stay.Result Blood calcium, LDH, BUN, pleural effusion, MCTSI, Ranson score and BISAP score were related to the length of stay (P <0.05). WBC, AST, blood glucose, PaCO 2 , triglyceride, PH, glycated hemoglobin, CRP, Cr , PCT were not related to the length of stay (P> 0.05).Conclusion The current Atlanta classification is not suitable for evaluating the severity of AP with DKA. Serum calcium, LDH, BUN, pleural effusion, and MCTSI are suitable for evaluating the length of stay and severity of AP with DKA.