bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating the role of predatory fish controlling the invasion of the Asian golden mussel Limnoperna fortunei in a subtropical river
Authors: González‐Bergonzoni Ivan , Silva Ivana , Teixeira de Mello Franco , D'Anatro Alejandro , Boccardi Lucia , Stebniki Samanta , Brugnoli Ernesto , Tesitore Giancarlo , Vidal Nicolás , Naya Daniel E. 
Published: January 2020

Abstract

The invasive Asian golden mussel Limnoperna fortunei is continuously expanding through South America, altering aquatic ecosystem structure and functioning. While several native fish species predate on this mussel, these interactions, and their consequences for the food web, have not been studied in depth.

We combine a survey of the fish assemblage trophic structure using gut contents and stable isotope analysis with an in situ exclusion experiment in order to: (a) determine the main fish species predating on L. fortunei; (b) estimate the contribution of L. fortunei to the biomass of fish assemblage; and (c) evaluate the role of fish on the density and maximum shell length of L. fortunei in the lower Uruguay River (the Río de La Plata Basin).

We found that about one third of fish species in the assemblage (28 out of 81 species) consumed L. fortunei, which was an important food item (>10% of the dietary volume and >30% of frequency of occurrence) for 11 fish species. Stable isotope mixing models consistently suggested that >14% of the total biomass of the fish assemblage was derived directly from carbon derived from L. fortunei tissues. In addition, the exclusion experiment demonstrated that fish predation reduces the mussel density by c. 70% and the mussel maximum shell length by c. 40%.

Synthesis and applications. Our research suggests a top‐down effect of fish on the invasive Asian golden mussel's abundance. This work highlights the need to preserve the native fish communities, particularly in South America, where many vulnerable migratory fish are of key relevance as Limnoperna fortunei consumers. In the light of these findings, actions such as implementing efficient fish passage technologies in the actual (and projected) dams of the Uruguay River basin and elsewhere become essential to avoid local extinctions of these natural invasion controls upstream of the dams.
The effect of environmental gradient on biodiversity and similarity of invertebrate communities in eelgrass (Zostera marina) beds
Authors: Namba Mizuho , Hashimoto Marina , Ito Minako , Momota Kyosuke , Smith Carter , Yorisue Takefumi , Nakaoka Masahiro 
Published: January 2020

AbstractEnvironmental filtering and dispersal limitation are important processes within the metacommunity concept. Non‐random species turnover occurs in places where environmental filtering plays the key role in determining local community structure, whereas dispersal limitation causes nested patterns of species assemblages organized by non‐random colonization processes. However, factors that modify the relative importance of these processes remain unclear for many ecosystems. We tested whether salinity gradient affect the relative importance of environmental filtering and dispersal limitation for structuring epifaunal and infaunal communities in three lagoons in Hokkaido, Japan, that have different salinity gradients. Specifically, we compared patterns of species diversity and similarity of eelgrass‐associated invertebrate assemblages across space. Beta diversity (i.e., species turnover among different sites in each lagoon) was highest in Akkeshi, the lagoon with the salinity gradients. Variation partitioning of similarity components showed that spatial variation in the community assemblage pattern was mostly explained by environmental filtering in Akkeshi, but that it was explained more by species dispersal patterns and the difference in eelgrass biomass and shoot density in Notoro and Saroma, the lagoons without clear salinity gradient. Redundancy analysis showed that spatial variation in community structure was related to salinity and eelgrass biomass in Akkeshi, and to eelgrass aboveground biomass in Notoro and Saroma. Our findings highlight the effects of environmental heterogeneity on beta diversity and community structure and indicate that environmental gradients can be a key factor causing a shift in the relative importance of different metacommunity processes and the role of the foundation species in provisioning habitat.
Culture‐independent tracking of Vibrio cholerae lineages reveals complex spatiotemporal dynamics in a natural population
Authors: Kirchberger Paul C. , Orata Fabini D. , Nasreen Tania , Kauffman Kathryn M. , Tarr Cheryl L. , Case Rebecca J. , Polz Martin F. , Boucher Yann F. 
Published: January 2020

SummaryPopulations of the bacterium Vibrio cholerae consist of dozens of distinct lineages, with primarily (but not exclusively) members of the pandemic generating lineage capable of causing the diarrhoeal disease cholera. Assessing the composition and temporal dynamics of such populations requires extensive isolation efforts and thus only rarely covers large geographic areas or timeframes exhaustively. We developed a culture‐independent amplicon sequencing strategy based on the protein‐coding gene viuB (vibriobactin utilization) to study the structure of a V. cholerae population over the course of a summer. We show that the 26 co‐occurring V. cholerae lineages continuously compete for limited space on nutrient‐rich particles where only a few of them can grow to large numbers. Differential abundance of lineages between locations and size‐fractions associated with a particle‐attached or free‐swimming lifestyle could reflect adaptation to various environmental niches. In particular, a major V. cholerae lineage occasionally grows to large numbers on particles but remain undetectable using isolation‐based methods, indicating selective culturability for some members of the species. We thus demonstrate that isolation‐based studies may not accurately reflect the structure and complex dynamics of V. cholerae populations and provide a scalable high‐throughput method for both epidemiological and ecological approaches to studying this species.
Human papillomavirus infection maybe not associated with primary lung cancer in the Fujian population of China
Authors: He Fei , Xiong Weimin , Yu Fanglin , Xiao Rendong , Ye Hailing , Li Wenjun , Liu Zhiqiang , Hu Zhijian , Cai Lin 
Published: January 2020

BackgroundTo investigate whether human papillomavirus (HPV) infection is associated with primary lung cancer among the Fujian population.MethodsHPV infection was detected in 140 pairs of lung cancer tissues and matched paracancerous tissues by examining the 21 clinically relevant HPV types using a combination of viral highly conserved L1 region PCR amplification and specific probe reverse hybridization. Paired χ2 test was used to analyze differences in detection rates of HPV between lung cancer and paracancerous tissues. Differences in detection rates of HPV in lung cancer tissues were analyzed using χ2 test or the exact probability method. The rank sum test was used to analyze differences in the distributions of routine indices of blood and pulmonary function in lung cancer tissues between the HPV negative and positive groups.ResultsHPV infection was detected in 13 of the 140 tumor specimens and in 16 of the paired normal lung tissues. There was no significant correlation between HPV infection and lung cancer (P > 0.05). The diagnosed HPV infection rates did not differ significantly among lung cancer tissues with different stratification (P > 0.05). However, the platelet count, platelet pressure, residual gas volume, functional residual volume, and residual gas volume/lung total distribution may differ between HPV‐negative and HPV‐positive lung cancer tissues (0.000625 < P < 0.05).ConclusionsWe concluded that HPV infection may not be associated with the risk of primary lung cancer in the Fujian population. However, HPV infection may affect platelet and residual lung function in primary lung cancer patients.
Long non‐coding RNA XIST expedites lung adenocarcinoma progression through upregulating MDM2 expression via binding to miR‐363‐3p
Authors: Rong Hao , Chen Bing , Wei Xing , Peng Jun , Ma Ke , Duan Song , He Jintao 
Published: January 2020

AbstractBackgroundLung adenocarcinoma (LAD) is a highly aggressive malignant tumor which threatens the health and life of the population. Long non‐coding RNA X‐inactive specific transcript (XIST) and mouse double minute clone 2 (MDM2) are connected with the tumorigenesis of LAD. Nevertheless, whether MDM2 is regulated by XIST has not previously been reported in LAD.MethodsQuantitative real‐time polymerase chain reaction (qRT‐PCR) was employed to detect the expression of XIST, microRNA‐363‐3p (miR‐363‐3p) and MDM2 in LAD tissues and cells. The proliferation, migration, invasion and apoptosis of LAD cells were determined by 3‐(4, 5‐dimethylthiazol‐2‐YI)‐2, 5‐diphenyltetrazolium bromide (MTT), transwell or flow cytometry assay, respectively. MDM2 protein level was detected using western blot analysis. Dual‐luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pulldown assay were performed to determine the interaction among XIST, miR‐363‐3p and MDM2. A xenograft tumor model was constructed to validate the effect of XIST on LAD cells in vivo.ResultsWe found that XIST and MDM2 were remarkably elevated while miR‐363‐3p was reduced in LAD tissues and cells. Both XIST and MDM2 downregulation restrained proliferation, migration and invasion, and facilitated apoptosis of LAD cells in vitro. Importantly, XIST bound to miR‐363‐3p to modulate MDM2 expression in LAD cells. Moreover, miR‐363‐3p knockdown or MDM2 elevation reversed the effects of XIST downregulation on the proliferation, migration, invasion and apoptosis of LAD cells. Furthermore, XIST knockdown constrained tumor growth on LAD cells in vivo.ConclusionsXIST knockdown repressed proliferation, migration and invasion, and accelerated apoptosis of LAD cells by downregulating MDM2 expression via binding to miR‐363‐3p.Key pointsSignificant findings of the study
XIST and MDM2 were abnormally enhanced in LAD tissues and cells.
Both downregulation of XIST and MDM2 repressed proliferation, migration and invasion, and boosted apoptosis of LAD cells in vitro.
XIST bound to miR‐363‐3p to regulate MDM2 expression in LAD cells.

Downregulation of XIST impeded tumor growth on LAD cells in vivo.
What this study adds
This study confirmed that XIST was a potential target for inhibiting the development of LAD, and affords a possible strategy for the treatment of LAD in the future.
Correlation between MDM2 T309G single nucleotide polymorphism and esophageal cancer susceptibility: An updated meta‐analysis
Authors: Yin Lele , Shen Guo , Zhu Bin 
Published: January 2020

BackgroundThe aim of this study was to investigate the correlation between MDM2 T309G single nucleotide polymorphism (SNP) and esophageal cancer susceptibility through pooling the open published data.MethodsBy systematic searching the databases of Medline, EMBASE, CBM and CNKI, the case‐control or cohort studies related to MDM2 T309G single nucleotide polymorphism and esophageal cancer risk were screened. Genetic phenotype data of T309G single nucleotide was extracted from the original included studies. The correlation between MDM2 T309G single nucleotide polymorphism and esophageal cancer susceptibility was demonstrated by the odds ratio (OR) and its corresponding 95% confidence interval (95% CI). Publication bias was investigated by Egger's line regression test and begg's funnel plot.ResultsAfter systematic searching of the relevant database, nine publications were finally included in the present study. The combined data demonstrated that the subjects with the G genotype had an increased risk of developing esophageal cancer in dominant (OR = 1.13, 95% CI: 1.00–1.27, P = 0.043), recessive (OR = 1.27, 95% CI: 1.12–1.45, P = 0.000) and homozygous (OR = 1.34, 95% CI:1.04–1.74, P = 0.024) genetic model through random or fixed data pooling method. Both begg's and Egger's line regression test indicated no significant publication bias.ConclusionBased on the present data, there was a significant correlation between MDM2 T309G single nucleotide polymorphism and esophageal cancer susceptibility. Individuals with G genotype may have an increased risk of developing esophageal cancer.
Long‐term incidence of dysplasia and colorectal cancer in an ulcerative colitis population‐based cohort
Authors: Tranter‐Entwistle Isaac , Mullaney Tamara G. , Noah Kimberley , Pearson John , Falvey James , Gearry Richard , Eglinton Tim 
Published: January 2020

AbstractBackgroundUlcerative colitis (UC) is a risk factor in developing colorectal cancer (CRC). Surveillance programmes aim to identify premalignant lesions to facilitate improved treatment outcomes. Recent studies have suggested that the risk of CRC in UC has decreased. This study aims to characterize the risk of CRC in UC in a population‐based New Zealand cohort.MethodsAll patients in the Canterbury Inflammatory Bowel Disease Study, a comprehensive population‐based cohort, were reviewed and cases of dysplasia and CRC identified. Demographic data and risk factors were assessed and standardized incidence ratios (SIRs) calculated comparing with the national population.ResultsA total of 518 UC cases were analysed (46.3% female). Median follow‐up was 17.5 years (interquartile range 12.2–25.1 years). Neoplasia developed in 42 (8.1%) patients, 14 (2.7%) of whom had CRC. The mean age at CRC diagnosis was 63.3 years, and mean duration with UC before CRC 18.4 years (0–36.8 years). The total incidence rate was 1.35/1000 person‐year duration (95% confidence interval 0.74–2.27). The age‐adjusted SIR was 1.74 (95% confidence interval 1.03–2.93) compared to the New Zealand population. Risk factors for any dysplasia included disease extent and male gender.ConclusionIn this population‐based cohort with long‐term follow‐up, the SIR of CRC in UC patients was significantly lower than the initial epidemiological studies although similar to more recent studies. This increased risk still justifies ongoing screening in the UC population.
Computational modeling of the Food and Drug Administration’s benchmark centrifugal blood pump
Authors: Good Bryan C. , Manning Keefe B. 
Published: January 2020

AbstractIn order to simulate hemodynamics within centrifugal blood pumps and to predict pump hemolysis, CFD simulations must be thoroughly validated against experimental data. They must also account for and accurately model the specific working fluid in the pump, whether that is a blood‐analog solution to match an experimental PIV study or animal blood in a hemolysis experiment. Therefore, the Food and Drug Administration (FDA) benchmark centrifugal blood pump and its database of experimental PIV and hemolysis data were used to thoroughly validate CFD simulations of the same blood pump. A Newtonian blood model was first used to compare to the PIV data with a blood analog fluid while hemolysis data were compared using a power‐law hemolysis model fit to porcine blood data. A viscoelastic blood model was then incorporated into the CFD solver to investigate the importance of modeling blood’s viscoelasticity in centrifugal pumps. The established computational framework, including a dynamic rotating mesh, animal blood‐specific fluid properties and hemolysis modeling, and a k‐ω SST turbulence model, was shown to more accurately predict pump pressure heads, velocity fields, and hemolysis compared to previously published CFD studies of the FDA centrifugal pump. The CFD simulations were able to match the FDA pressure and hemolysis data for multiple pump operating conditions, with the CFD results being within the standard deviations of the experimental results. While CFD radial velocity profiles between the impeller blades also compared well to the PIV velocity results, more work is still needed to address the large variability among both experimental and computational predictions of velocity in the diffuser outlet jet. Small differences were observed between the Newtonian and viscoelastic blood models in pressure head and hemolysis at the higher flow rate cases (FDA Conditions 4 and 5) but were more significant at lower flow rate and pump impeller speeds (FDA Condition 1). These results suggest that the importance of accounting for blood’s viscoelasticity may be dependent on the specific blood pump operating conditions. This detailed computational framework with improved modeling techniques and an extensive validation procedure will be used in future CFD studies of centrifugal blood pumps to aid in device design and predictions of their biological responses.
Periodontal disease in free‐ranging koalas (Phascolarctos cinereus) from the Mount Lofty Ranges, South Australia, and its association with koala retrovirus infection
Authors: Butcher RG , Pettett LM , Fabijan J , Ebrahimie E , Mohammadi‐Dehcheshmeh M , Speight KN , Boardman WSJ , Bird PS , Trott DJ 
Published: January 2020

BackgroundIn northern Australian koala populations (Queensland and New South Wales), periodontal disease (gingivitis and periodontitis) is common while koala retrovirus subtype A is endogenous, with other subtypes transmitted exogenously. Koala retrovirus has been hypothesised to cause immune suppression and may predispose koalas to diseases caused by concurrent infections. In southern Australia populations (Victoria and South Australia) periodontal disease has not been investigated, and koala retrovirus is presumably exogenously transmitted. This study described oral health in South Australian koalas and investigated if an association between periodontal disease and koala retrovirus exists.MethodsOral health was examined for wild‐caught koalas from the Mount Lofty Ranges (n = 75). Koala retrovirus provirus was detected in whole blood using nested PCR and proviral load determined with qPCR. Periodontal disease severity was recorded and used to calculate the Final Oral Health Index (0‐normal, 24‐severe).Results Periodontal disease was observed in 84% (63/75) of koalas; 77% had gingivitis (58/75) and 65% (49/75) had periodontitis. The average Final Oral Health Index was 5.47 (s.d 3.13). Most cases of periodontal disease were associated with the incisors. Koala retrovirus‐infected koalas were more likely to present with periodontitis (p = 0.042) and the Final Oral Health Index was negatively correlated with proviral load (ρ = −0.353, p = 0.017).ConclusionSouth Australian koalas had a high prevalence of gingivitis and periodontitis. Periodontal disease was more prevalent in the incisors. Exogenous koala retrovirus infection may also facilitate the development of periodontitis by modulation of the immune response to concurrent oral bacterial infections.
Does context matter for sustainability disclosure? Institutional factors in Southeast Asia
Authors: Tran Mi , Beddewela Eshani 
Published: January 2020

AbstractCorporate sustainability is a dynamic, socially constructed concept. Relatedly, to understand the variations in the disclosure of corporate sustainability activities across countries, we need to inherently explore their underlying sociopolitical contexts. At present our understanding in this regard, is deficient. We respond to this extant research gap by adopting a multi‐country approach to investigate the relationship between countries' institutional environments and firms' sustainability disclosure (SD) practices, across six countries in the Southeast Asian region. Our findings reflect a common focus of Southeast Asian firms on community and human resources (HRs) related disclosures. Nevertheless, nuanced differences in their overall SD levels confirm the influence of differing legal, normative, and sociocultural systems in engendering greater disclosure and transparency at a national level. By quantifying the institutional environment and identifying external influencing factors, our study provides a useful framework grounded in neo‐institutional theory to widen the existing understanding of how institutional pressures can be measured and compared across different contexts.
Detecting community structure in complex networks using genetic algorithm based on object migrating automata
Authors: Zarei Bagher , Meybodi Mohammad Reza 
Published: January 2020

AbstractCommunity structure is an important topological feature of complex networks. Detecting community structure is a highly challenging problem in analyzing complex networks and has great importance in understanding the function and organization of networks. Up until now, numerous algorithms have been proposed for detecting community structure in complex networks. A wide range of these algorithms use the maximization of a quality function called modularity. In this article, three different algorithms, namely, MEM‐net, OMA‐net, and GAOMA‐net, have been proposed for detecting community structure in complex networks. In GAOMA‐net algorithm, which is the main proposed algorithm of this article, the combination of genetic algorithm (GA) and object migrating automata (OMA) has been used. In GAOMA‐net algorithm, the MEM‐net algorithm has been used as a heuristic to generate a portion of the initial population. The experiments on both real‐world and synthetic benchmark networks indicate that GAOMA‐net algorithm is efficient for detecting community structure in complex networks.
Effects of isometric handgrip training on cardiac autonomic profile: A systematic review and meta‐analysis study
Authors: Farah Breno Q. , Christofaro Diego G. D. , Correia Marilia A. , Oliveira Crystian B. , Parmenter Belinda J. , Ritti‐Dias Raphael M. 
Published: January 2020

AbstractMeta‐analyses have shown that isometric handgrip training reduces blood pressure in normotensive and hypertensive subjects. However, the effects on cardiac autonomic modulation are still controversial. Thus, the aim of this systematic review and meta‐analysis was to analyse the effects of isometric handgrip training on cardiac autonomic modulation in normotensive and hypertensive subjects. For this, Medline, Cinhal, Embase, Spordiscus and PEdro were searched for relevant studies published until December 2018. Randomized controlled trials investigating the effect of isometric handgrip training on heart rate variability parameters were considered eligible. Parameters were obtained in time (standard deviation of all the RR intervals‐SDNN, root mean square of successive differences between the normal adjacent RR intervals‐RMSSD and the percentage of adjacent intervals with more than 50 ms‐PNN50) and frequency domain (low frequency‐LF, high frequency‐HF and sympathovagal balance‐LF/HF). Mean difference (MD) and 95% confidence interval (95% CI) were calculated using an inverse variance method with a random effects model. Seven trials were included in the systematic review and meta‐analysis, totalling 86 participants. No significant effect was observed in heart rate variability parameters after isometric handgrip training (4 trials to SDNN: MD = −1.44 ms and 95% CI = −8.02, 5.14 ms; RMSSD: MD = −1.48 ms and 95% CI = −9.41, 6.45 ms; PNN50: MD = 0.85% and 95% CI = −1.10, 2.81%; 7 trials to LF: −0.17 n.u. and 95% CI = −6.32, 5.98 n.u.; HF: MD = 0.17 n.u. and 95% CI = −5.97, 6.30 n.u.; and LF/HF: MD = 0.13 and 95% CI = −0.34, 0.59). In conclusion, current literature indicates that isometric handgrip training does not improve heart rate variability.