bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in clinical and imaging features of low lumbar osteoporotic vertebral collapse in patients with or without Parkinson’s disease: Rapid destructive progression and appearance of a cauda equina sign in Parkinson’s disease patients
Authors: Nakajima Hideaki , Kubota Arisa , Watanabe Shuji , Honjoh Kazuya , Matsumine Akihiko 
Published: October 2020

Abstract
BackgroundOsteoporosis and Parkinson’s disease (PD) are age-related diseases, and surgery for osteoporotic vertebral collapse (OVC) in PD patients become more common with aging of society. OVC commonly affects the thoracolumbar spine, but low lumbar OVC is frequent in patients with lower bone mineral density (BMD) and a higher mechanical failure rate, compared with those with thoracolumbar junction collapse. The aim of this study was to identify differences in clinical and imaging features, and in outcomes of low lumbar OVC with or without PD and to discuss the appropriate treatment for lower lumbar OVC in patients with PD.MethodsThe subjects were 43 patients with low lumbar OVC below L3 who were treated surgically, including 11 patients with PD. Clinical symptoms, morphological features of affected vertebrae, neurological status, surgical procedures, and complications were compared in patients with and without PD.ResultsThe main clinical symptoms were radicular leg pain in non-PD cases (68.8%) and a cauda equina sign in PD cases (72.7%). Rapid progression and destructive changes of OVC were seen in patients with PD at 24.5 ± 10.5 days after injury. The morphological features of OVC were flat-type in non-PD cases with old compression fracture at the thoracolumbar lesion, and destruction-type in PD cases without old compression fracture. Progression of PD was associated with decreased lumbar lordosis, lower lumbar YAM, and severe sarcopenia, all of which can affect postoperative instrumentation-related complications. High postoperative complication rates may be due to vertebral fragility and longer fusion surgery. ConclusionsRapid progression and destructive changes of low lumbar OVC may occur in PD patients, and significantly more PD cases have a cauda equina sign and require urgent surgery. Progression of postural instability as a natural course of PD may lead to mechanical stress and instrumentation failure, especially at an upper adjacent level. Given the progression of PD after surgery, invasive long-fusion surgery should be avoided for single low lumbar OVC. A surgical strategy considering the severities of PD and osteoporosis, and aggressive control of PD before and after surgery are important to prevent complications.
Host Microbiome Dictates Infection Risk after Chemotherapy Revealing New Avenues for Antibiotic Stewardship in Oncology
Authors: Wardill Hannah R , Mooij Charlotte EM de , Ferreira Ana Rita Da Silva , Peppel Ivo P van de , Havinga Rick , Harmsen Hermie JM , Tissing Wim JE , Blijlevens Nicole MA 
Published: October 2020

Abstract
Background: Mucosal barrier injury (MBI) is a recognized risk factor for blood stream infection (BSI) in people undergoing chemotherapy, permitting expansion and translocation of enteric pathobionts; a phenomenon now clinically referred to as MBI-associated laboratory-confirmed BSI. Although recognized to originate from endogenous gastrointestinal sources, MBI-associated BSI continue to be treated with antibiotics; a counterintuitive approach that may increase infection risk by depletion of the host microbiome. While this point has been argued in numerous clinical studies and opinion pieces, there are few data describing mechanistic strategies to decrease MBI-associated BSI, reflecting a lack of translationally robust preclinical models. Here, we report on a new translational model of MBI caused by the chemotherapeutic drug, melphalan. We aimed to identify candidate pathways to strengthen the mucosal barrier and prevent infection, prompting new antibiotic stewardship initiatives. Results: Melphalan caused dose-dependent, systemic toxicity characterized by severe neutropenia, self-limiting intestinal injury, inflammation and biphasic fever, all of which were clinically and molecularly consistent with the dynamics of melphalan conditioning. The fecal microbiome following melphalan was depleted in richness and commensal taxa, impairing colonization resistance and the microbial metabolome and prompting expansion of enteric pathogens. Breakdown of the intestinal barrier was initiated by melphalan and exacerbated by mucotoxic bile acids.Conclusions: MBI-associated BSI is simply a form of collateral damage resulting from breakdown of the gastrointestinal microenvironment and self-perpetuating injury. Efforts to intervene early in these sequelae are therefore of great clinical significance to restrict antibiotic use and mitigate their detrimental consequences on treatment outcomes and antibiotic resistance. Our data support interventions targeting the host microbiome and metabolome due to its ubiquitous control of inflammation, mucosal injury, bile acid transformation; all of which contribute to infection risk in cancer.
A Multiplexed, Next Generation Sequencing Platform for High-Throughput Detection of SARS-CoV-2
Authors: Wrana Jeff , Aynaud Marie-Ming , Hernandez J , Barutcu Seda , Braunschweig Ulrich , Chan Kin , Pearson Joel , Trcka Daniel , Prosser Suzanna , Kim Jaeyoun , Barrios-Rodiles Miriam , Jen Mark , Song Siyuan , Shen Jess , Bruce Christine , Hazlett Bryn , Poutanen Susan , Attisano Liliana , Bremner Rod , Blencowe B , Mazzulli Tony , Han Hong , Pelletier Laurence 
Published: October 2020

Abstract
Population scale sweeps of viral pathogens, such as SARS-CoV-2, that incorporate large numbers of asymptomatic or mild symptom patients present unique challenges for public health agencies trying to manage both travel and local spread. Physical distancing is the current major strategy to suppress spread of the disease, but with enormous socio-economic costs. However, modelling and studies in isolated jurisdictions suggest that active population surveillance through systematic molecular diagnostics, combined with contact tracing and focused quarantining can significantly suppress disease spread and has significantly impacted disease transmission rates, the number of infected people, and prevented saturation of the healthcare system. However, reliable systems allowing for parallel testing of 10-100,000’s of patients in larger urban environments have not yet been employed. Here we describe “COVID-19 screening using Systematic Parallel Analysis of RNA coupled to Sequencing” (C19-SPAR-Seq), a scalable, multiplexed, readily automated next generation sequencing (NGS) platform that is capable of analyzing tens of thousands of COVID-19 patient samples in a single instrument run. To address the strict requirements in clinical diagnostics for control of assay parameters and output, we employed a control-based Precision-Recall and predictive Receiver Operator Characteristics (coPR) analysis to assign run-specific quality control metrics. C19-SPAR-Seq coupled to coPR on a trial cohort of over 600 patients performed with a specificity of 100% and sensitivity of 91% on samples with low viral loads and a sensitivity of > 95% on high viral loads associated with disease onset and peak transmissibility. Our study thus establishes the feasibility of employing C19-SPAR-Seq for the large-scale monitoring of SARS-CoV-2 and other pathogens.
Exploration of High-Intensity Interval Training for Breast Cancer Survivors: Taking a Home-based Approach Toward New Normal
Authors: Tsuji Katsunori , Matsuoka Yutaka J. , Ochi Eisuke 
Published: October 2020

Abstract
BackgroundTo review the methods and outcomes of high-intensity interval training (HIIT) interventions for breast cancer survivors, and to explore the feasibility of prescribing exercise for breast cancer survivors.MethodsA systematic search of electronic databases was conducted for studies published up to May 31, 2020. Eligibility criteria included randomized controlled trials of HIIT intervention in breast cancer survivors. Studies were grouped by whether the intervention was conducted during or after breast cancer treatment, and intervention methods and outcomes were reviewed within each group.Results Twenty-six studies were identified, and 13 satisfied the inclusion criteria. Intervention was conducted during treatment in 8 studies, and after treatment in 5. Intervention duration ranged from 3 to 16 weeks, with 2 or 3 sessions per week, for a total of 9 to 36 sessions. All interventions were supervised; 12 were lab-based, and 1 was community-based. One of most promising outcomes was improvement of cardiorespiratory fitness by HIIT.ConclusionThis review found that most studies on HIIT for breast cancer survivors investigated lab-based, supervised interventions. HIIT is a time-efficient method for increasing cardiovascular function in breast cancer survivors, but further research is necessary to determine its effects on other outcomes.
The Critical Role of PTEN Mutation in Cellular Process and Drug Selection of Endometrial Cancer
Authors: Liu Ying , Zhou Jintuo , Niu Peiguang , Zeng Fanxiang , Cai Ruihong , Deng Jie , Lin Wei , Zeng Xiaoping , Shi Daohua 
Published: October 2020

Abstract
Background: Phosphatase and tensin homolog (PTEN) is a frequently mutated genes found in endometrial cancer (EC), making it a potential biomarker for individualized treatment opinions. In this study, a method was designed to evaluate the role of the PTEN mutation in the prognosis and drug selection of EC. We identified the potential alterations in pathways and genes related to the mechanism. Methods: cBioPortal database was used to analyze the PTEN mutation status for EC patients. Kaplan-Meier was used to analyze the prognosis of PTEN mutation in EC patients. GDSC dataset was used to identified the drugs that sensitive to cell lines with PTEN mutation. DEGs between PTEN mutation and wide type group were identified using the edgeR package. GO and KEGG analysis were carried out using the DAVID database. GSEA v3.0 were used to dig out the differences in gene mRNA levels of biological function annotation and pathways between PTEN mutation and wide type patients. PPI network of DEGs was performed using STRING and then visualized using Cytoscope software (3.7.2).Results: Our results showed that PTEN mutation was carried in 68% of EC patients. The mRNA expression level of PTEN was lower in patients with PTEN mutation than that with wide type. Prognosis analysis showed that there were favorable overall survival and progression free survival in EC patients with PTEN mutation. Moreover, it is more sensitive to AKT inhibitor (Afuresertib and AZD5363), and Mcl-1 inhibitor (MIM1) on EC cell lines with PTEN mutation than that with wide type. A total of 216 genes were identified as DEGs. GO analysis showed that DEGs significantly enriched in chemical synaptic transmission, extracellular region, etc.. KEGG analysis suggested that DEGs significantly enriched in categories associated with metabolic progression. GSEA analysis identified signaling pathways including fatty acid metabolism, fructose and mannose metabolism, etc.. PPI network analysis identified top 10 genes and top three clusters.Conclusions: Multiple genes and pathways may play an important role in EC patients with PTEN mutation. These results provide a potential target and therapeutic strategies for patients with PTEN mutation.
The Protective Effects of Phosphodiesterase-5 Inhibitor, Sildenafil on Post-resuscitation Cardiac Dysfunction of Cardiac Arrest: By Regulating the MiR-155-5p and MiR-145-5p
Authors: He Yong , Wang Guoxing , Li Chuang , WANG YUXING , ZHANG QIAN 
Published: October 2020

Abstract
Background: MiRNA-155 and miRNA-145 have been demonstrated to function as a key regulator in the development of the cardiovascular system. Recent experimental and clinical studies have indicated the cardioprotective role of sildenafil during ischemia/reperfusion (I/R) injury. This study was designed to investigate if administration of sildenafil will attenuate post-resuscitation myocardial dysfunction by regulating miRNA-155 and miR-145 expressions.Methods: Thirty-two male pigs (weighing 30 ± 2 kg) were randomly divided into 4 groups, sildenafil group (n= 8), sildenafil +NG-nitro-l-arginine methyl ester (L-NAME) (20mg/kg L) group (n=8), saline (SA group, n=8); and sham operation group (sham group, n=8). Eight minutes of untreated VF was followed by defibrillation in anesthetized, closed-chest pigs. Hemodynamic status and blood samples were obtained at 0 min, 0.5, 1, 2, 4 and 6h after return of spontaneous circulation (ROSC), and the hearts were removed and analyzed under electron microscopy, quantitative real-time polymerase chain reaction and ultra structural analysis were performed to evaluate myocardial injury.Results: Compared with the sildenafil + L-NAME and saline groups, the sildenafil group had better outcomes in terms of hemodynamic and oxygen metabolism parameters as well as 24-hour survival rate, and attenuated myocardial injury; In this study, CA pigs showed evidently increased levels of miR-155-5p and miR-145-5p, while the sildenafil treatment decreased the levels of miR-155-5p and miR-145-5p in CA pigs. In addition, the levels of eNOS was decreased in CA pigs, validating sildenafil attenuating post-resuscitation myocardial dysfunction by regulating miRNA-155 and miR-145 expressions.Conclusions: sildenafil group had better outcomes in terms of hemodynamic and oxygen metabolism parameters as well as 24-hour survival rate, inhibited the increases in the miR-155-5p and miR-145-5p levels and attenuated myocardial injury in a porcine model of CA and resuscitation.
A Multi-level Hypoglycemia Early Alarm System Based on Sequence Pattern Mining
Authors: Yu Xia , Ma Ning , Yang Tao , Zhang Yawen , Miao Qing , Tao Junjun , Li Hongru , Li Yiming , Yang Yehong 
Published: October 2020

Abstract
Background: Early alarm of hypoglycemia, detection of asymptomatic hypoglycemia, and effective control of blood glucose fluctuation make a great contribution to the treatment for diabetic patients. In this study, we designed a multi-level hypoglycemia early alarm system to improve the overall performance of hypoglycemia early alarm. Methods: Through symbolizing the historical CGM data, the Prefix Span was adopted to obtain the early alarm and non-alarm frequent sequence libraries of hypoglycemia. The longest common subsequence was used to remove the common frequent sequence for achieving the hypoglycemia early alarm on the clinical dataset. Frequent sequence pattern libraries with different risk levels were designed by choosing different thresholds and a multi-level hypoglycemia early alarm system for different clinical situations were established. Results: The model was able to predict hypoglycemia events in the clinical dataset of level-I (sensitivity 85.90%, false-positive 23.86%, miss alarm rate 14.10%, average early alarm time 20.61min), level-II (sensitivity 80.36%, false-positive 17.37%, miss alarm rate 19.63%, average early alarm time 27.66min), and level-III (sensitivity 78.07%, false-positive 13.59%, miss alarm rate 21.93%, average early alarm time 33.80min), respectively.Conclusions: The proposed approach could effectively predict hypoglycemia events on the basis of different thresholds to meet different prevention and treatment requirements in clinical situations. Moreover, the experimental results confirm the practicality and prospects of the proposed early alarm system, which reflects further significance in personalized medicine for hypoglycemia prevention.
The International Society of Ultrasound in Obstetrics and Gynecology (ISUOG) Basic Training Course: Evaluation in China
Authors: Wang Jingjing , Li Lin , Xuan Yinghua , Wang Li , Wu Qingqing 
Published: October 2020

Abstract
BackgroundThe aim of this study is to evaluate effectiveness of ISUOG (International Society of Ultrasound in Obstetrics and Gynecology) basic training curse in Chinese sonographer, which has not been reported yet. MethodsISUOG basic training course includes 30 lectures and was held in 3 days in 2019 in Beijing, China. We included the questionnaires answered by the trainees both before and after the course in each day. There were 9 lectures presented in the first day, 12 in the second and 9 in the last. The theoretical tests were provided by ISUOG, including 22, 21 and 21 multiple-choice questions respectively in 3 days. Pre and post-training theoretical test scores were compared by Wilcoxon singned-rank test. The changes of the theoretical test scores and the rates of “very satisfied” and “very useful” from trainees of different professional titles were compared by Wilcoxon singned-rank test and Chi-square test.ResultsQuestionnaires from 481 trainees who have experience of ultrasonic scan in Ob/Gyn and also had done the survey both before and after course were collected, with 274 in the first day, 94 in the second and 113 in the last. The differences of the correct-answer-number got from the before-test and after-test were with statistically significance(all P-values <0.05). The trainees who got better scores after the training were 219(79.9%), 73(77.7%) and 82(72.6%) in each day, and the changes of scores were without significant difference between doctors of different titles( all P-values >0.05). After every day’s training, the rates as “very satisfied” evaluated by trainees were 81.4%, 87.2% and 91.1% respectively, and the rates as “very useful” were 80.3%, 88.3% and 91.2%. The rate of “very useful” evaluated by trainees of primary title was higher than that of high title, with P-values of 0.01.ConclusionOur results indicated that ISUOG basic training course in China got good feedback and could assist Chinese sonographers in some degree.
Persian Version of the Barkin Index of Maternal Functioning (BIMF): A Cross-Cultural Adaptation and Psychometric Evaluation
Authors: Ansariniaki Mehri , Lamyian Minoor , Ahmadi Fazlollah , Foroushani Abbas Rahimi , Curry Carolann L. , Barkin Jennifer L. 
Published: October 2020

Abstract
BackgroundPostpartum maternal functioning has the potential to affect the quality of interaction between mother and child. A proper assessment of maternal functioning requires a comprehensive and accurate tool. The objective of this study was to prepare a Persian version of the Barkin Index of Maternal Functioning (BIMF) and evaluate its psychometric properties in order to determine its applicability in Iranian mothers.MethodsThe BIMF was translated into Persian and then culturally adapted for Iranian women. After evaluating face and content validity, a cross-sectional study was conducted using the Persian version of BIMF. The data was collected from two unique groups of 250 mothers (in all 500 mothers); the women had infants who were 2 to 12-months old and were selected using a two-stage cluster sampling method. Factor analysis, Pearson’s correlation, intra-class correlation coefficients (ICC), composite reliability (CR) and Cronbach’s alpha were employed in order to evaluate structural validity and reliability.ResultsExploratory factor analysis resulted in a five-factor structure consisting of 20 items. Subsequently, confirmatory factor analysis (X ²/ df= 1.61, RMSEA = 0.050, GFI = 0.91, CFI = 0.91) confirmed that the Persian version had satisfactory goodness of fit. Reliability and internal consistency were confirmed with a CR of 0.77, an ICC of 0.87 and a Cronbach's alpha of 0.81.ConclusionsThe findings indicated that the Persian version of the BIMF is a valid and reliable instrument for assessing maternal functioning among Iranian mothers.
Usefulness of Intraoperative C-Arm Image Intensifier in Reducing Errors of Acetabular Component During Primary Total Hip Arthroplasty: An Application of Widmer’s Method
Authors: Song Joo-Hyoun , Kim Yong-Sik , Kwon Soon-Yong , Lim Young-Wook , Jung Jiyoung , Oh Seungbae 
Published: October 2020

Abstract
BackgroundAcetabular prosthesis positioning in total hip arthroplasty (THA) is crucial in reducing the risk of dislocation. There has been minimal research on the proper way to put the acetabular components into the safe zone intraoperatively. Assessment of version by intraoperative imaging intensifier is very valuable. The value of Widmer’s method, using the intraoperative C-arm available to determine cup anteversion was assessed.Methods101 hips in 91 patients who underwent primary THA were eligible for inclusion. Utilizing intraoperative C-arm images, measurement was performed using the technique described by Widmer. The values obtained using 3D computed tomography postoperatively, which determined the anteversion of the acetabular component, were regarded as the reference standard.ResultsThe method of Widmer obtained values similar to those obtained using 3D computed tomography and was considered accurate (n.s.). All 101 hips were positioned in the set target zone. Among the 101 hips, the cup position in nine hips (8.9%) was changed. The dislocation rate in our study was 1.0% with all dislocations occurring in hips placed in the target zone. The mean Harris hip score after THA in one year was 94.2 (82-98).ConclusionsThe method of Widmer was accurate using intraoperative imaging intensifier for the measurement of the anteversion of the acetabular component during THA, with reference to the anteversion obtained from the 3D computed tomography. Also, utilizing intraoperative C-arm imaging was very useful because it allowed for correction of the position of the acetabular cup.
FCN3 Is a Prognostic Biomarker Correlated with Immune Response in Liver Cancer
Authors: Lu Zhongxiao , Wu Jian , Li Yi-ming , Chen Wen-xiang , Yu Qiang-feng , Zhou Jian-Yin 
Published: October 2020

Abstract
AimLiver cancer is a common malignant tumor whose molecular pathogenesis remains unclear. This study attempts to identify key genes related to liver cancer by bioinformatics analysis and analyze their biological functions.MethodsThe gene expression data of the microarray were downloaded from the Gene Expression Omnibus(GEO) database. The differentially expressed genes (DEGs) were then identified by the R software package “limma” and were subjected to gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses using DAVID. The protein-protein interaction (PPI) network was constructed via String, and the results were visualized in Cytoscape. Modules and hub genes were identified using the MCODE plugin, while the expression of hub genes and its effects were analyzed by GEPIA2. Additionally, the co-expression of the hub gene was explored in String, while the GO results were visualized using the R software. Finally, the targets of the hub gene were predicted through an online website. ResultsIn total, 43 differentially expressed genes were obtained. The GO analysis was mainly concentrated in the redox process and nuclear mitosis, while the KEGG pathway analysis was mainly enriched in retinol metabolism and the cell cycle. Moreover, four hub genes were identified in the PPI network, however, the Kaplan-Meier risk curve showed that only ECT2 and FCN3 affected the survival of liver cancer. ECT2 was found to be high expressed in liver cancer, carrying out signal transduction and targeting hsa-miR-27a-3p. FCN3 was observed to be lowly expressed in liver cancer and related to the immune response, targeting hsa-miR132-5p.ConclusionThe obtained findings suggest that two genes are significantly related to the prognosis of liver cancer, and the analysis of their biological function provided novel insight into the pathogenesis of liver cancer. Furthermore, FCN3 may serve as a promising biomarker for patients with liver cancer.