bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal exercise conveys protection against NAFLD in the offspring via hepatic metabolic programming
Authors: Bae-Gartz Inga , Kasper Philipp , Großmann Nora , Breuer Saida , Janoschek Ruth , Kretschmer Tobias , Appel Sarah , Schmitz Lisa , Vohlen Christina , Quaas Alexander , Schweiger Michal R. , Grimm Christina , Fischer Axel , Ferrari Nina , Graf Christine , Frese Christian K. , Lang Sonja , Demir Münevver , Schramm Christoph , Fink Gregor , Goeser Tobias , Dötsch Jörg , Hucklenbruch-Rother Eva 
Published: September 2020

AbstractMaternal exercise (ME) during pregnancy has been shown to improve metabolic health in offspring and confers protection against the development of non-alcoholic fatty liver disease (NAFLD). However, its underlying mechanism are still poorly understood, and it remains unclear whether protective effects on hepatic metabolism are already seen in the offspring early life. This study aimed at determining the effects of ME during pregnancy on offspring body composition and development of NAFLD while focusing on proteomic-based analysis of the hepatic energy metabolism during developmental organ programming in early life. Under an obesogenic high-fat diet (HFD), male offspring of exercised C57BL/6J-mouse dams were protected from body weight gain and NAFLD in adulthood (postnatal day (P) 112). This was associated with a significant activation of hepatic AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor alpha (PPARα) and PPAR coactivator-1 alpha (PGC1α) signaling with reduced hepatic lipogenesis and increased hepatic β-oxidation at organ programming peak in early life (P21). Concomitant proteomic analysis revealed a characteristic hepatic expression pattern in offspring as a result of ME with the most prominent impact on Cholesterol 7 alpha-hydroxylase (CYP7A1). Thus, ME may offer protection against offspring HFD-induced NAFLD by shaping hepatic proteomics signature and metabolism in early life. The results highlight the potential of exercise during pregnancy for preventing the early origins of NAFLD.
ANFIS grid partition framework with difference between two sigmoidal membership functions structure for validation of nanofluid flow
Authors: Pishnamazi Mahboubeh , Babanezhad Meisam , Nakhjiri Ali Taghvaie , Rezakazemi Mashallah , Marjani Azam , Shirazian Saeed 
Published: September 2020

AbstractIn this study, a square cavity is modeled using Computational Fluid Dynamics (CFD) as well as artificial intelligence (AI) approach. In the square cavity, copper (Cu) nanoparticle is the nanofluid and the flow velocity characteristics in the x-direction and y-direction, and the fluid temperature inside the cavity at different times are considered as CFD outputs. CFD outputs have been assessed using one of the artificial intelligence algorithms, such as a combination of neural network and fuzzy logic (ANFIS). As in the ANFIS method, we have a non-dimension procedure in the learning step, and there is no issue in combining other characteristics of the flow and thermal distribution beside the x and y coordinates, we combine two coordinate parameters and one flow parameter. This ability of method can be considered as a meshless learning step that there is no instability of the numerical method or limitation of boundary conditions. The data were classified using the grid partition method and the MF (membership function) type was dsigmf (difference between two sigmoidal membership functions). By achieving the appropriate intelligence in the ANFIS method, output prediction was performed at the points of cavity which were not included in the learning process and were compared to the existing data (the results of the CFD method) and were validated by them. This new combination of CFD and the ANFIS method enables us to learn flow and temperature distribution throughout the domain thoroughly, and eventually predict the flow characteristics in short computational time. The results from AI in the ANFIS method were compared to the ant colony and fuzzy logic methods. The data from CFD results were inserted into the ant colony system for the training process, and we predicted the data in the fuzzy logic system. Then, we compare the data with the ANFIS method. The results indicate that the ANFIS method has a high potentiality compared to the ant colony method because the amount of R in the ANIFS system is higher than R in the ant colony method. In the ANFIS method, R is equal to 0.99, and in the ant colony method, R is equal to 0.91. This shows that the ant colony needs more time for both the prediction and training of the system. Also, comparing the pattern recognition in the two systems, we can obviously see that by using the ANFIS method, the predictions completely match the target points. But the other method cannot match the flow pattern and velocity distribution with the CFD method.
Formation of quantum dots in GaN/AlGaN FETs
Authors: Otsuka Tomohiro , Abe Takaya , Kitada Takahito , Ito Norikazu , Tanaka Taketoshi , Nakahara Ken 
Published: September 2020

AbstractGaN and the heterostructures are attractive in condensed matter science and applications for electronic devices. We measure the electron transport in GaN/AlGaN field-effect transistors (FETs) at cryogenic temperature. We observe formation of quantum dots in the conduction channel near the depletion of the 2-dimensional electron gas (2DEG). Multiple quantum dots are formed in the disordered potential induced by impurities in the FET conduction channel. We also measure the gate insulator dependence of the transport properties. These results can be utilized for the development of quantum dot devices utilizing GaN/AlGaN heterostructures and evaluation of the impurities in GaN/AlGaN FET channels.
Soluble urokinase plasminogen activator receptor level in individuals of advanced age
Authors: Wlazel Rafal N. , Szwabe Katarzyna , Guligowska Agnieszka , Kostka Tomasz 
Published: September 2020

AbstractSoluble urokinase plasminogen activator receptor (suPAR) is a biomarker whose clinical value has been tested in various groups of patients. The aim of the present study was to determine the suPAR level in a previously uninvestigated population of 182, generally healthy, community-dwelling seniors aged 74–89 years. In addition to suPAR level, selected laboratory parameters of heart and kidney function, lipid and C-reactive protein levels were determined. A group of 45 younger individuals aged 24–66 years was used for comparison. The seniors had higher suPAR levels than younger controls: 3.79 ng/mL (95% CI 3.64–3.96 ng/mL) vs. 3.16 ng/mL (95% CI 2.86–3.45 ng/mL). These levels increased further with advancing age, and were similar in women and men. A multiple regression model confirmed that biomarker level was related to cardiac function, renal function and inflammation, and this remained after adjusting for age. These correlation patterns were similar in older women and men.
Comparison of ultrasmall IONPs and Fe salts biocompatibility and activity in multi-cellular in vitro models
Authors: Janik-Olchawa Natalia , Drozdz Agnieszka , Ryszawy Damian , Pudełek Maciej , Planeta Karolina , Setkowicz Zuzanna , Śniegocki Maciej , Żądło Andrzej , Ostachowicz Beata , Chwiej Joanna 
Published: September 2020

AbstractIn the paper, the results of the first regular studies of ultra-small iron oxide nanoparticles (IONPs) toxicity in vitro were presented. The influence of PEG-coated NPs with 5 nm magnetite core on six different cell lines was examined. These were: human bronchial fibroblasts, human embryonic kidney cells (HEK293T), two glioblastoma multiforme (GBM) cell lines as well as GBM cells isolated from a brain tumor of patient. Additionally, mouse macrophages were included in the study. The influence of IONPs in three different doses (1, 5 and 25 µg Fe/ml) on the viability, proliferation and migration activity of cells was assessed. Moreover, quantifying the intracellular ROS production, we determined the level of oxidative stress in cells exposed to IONPs. In the paper, for the first time, the effect of Fe in the form of IONPs was compared with the analogical data obtained for iron salts solutions containing the same amount of Fe, on the similar oxidation state. Our results clearly showed that the influence of iron on the living cells strongly depends not only on the used cell line, dose and exposure time but also on the form in which this element was administered to the culture. Notably, nanoparticles can stimulate the proliferation of some cell lines, including glioblastoma multiforme. Compared to Fe salts, they have a stronger negative impact on the viability of the cells tested. Ultra-small NPs, also, more often positively affect cell motility which seem to differ them from the NPs with larger core diameters.
Antithymocyte globulin administration in patients with profound lymphopenia receiving a PBSC purine analog/busulfan-based conditioning regimen allograft
Authors: Jullien Maxime , Guillaume Thierry , Peterlin Pierre , Garnier Alice , Le Bourgeois Amandine , Debord Camille , Mahe Beatrice , Dubruille Viviane , Wuilleme Soraya , Blin Nicolas , Touzeau Cyrille , Gastinne Thomas , Tessoulin Benoit , Le Bris Yannick , Eveillard Marion , Duquesne Alix , Moreau Philippe , Le Gouill Steven , Bene Marie C. , Chevallier Patrice 
Published: September 2020

AbstractGraft-versus host disease (GVHD) remains one of the main causes of morbidity and mortality after allogeneic hematopoietic stem cell transplantation (ASCT). Prophylactic T cell depletion via antithymocyte globulin (ATG) during ASCT conditioning is one of the standards of care for GVHD prophylaxis, although the optimal dosing strategy is still unclear. Recent studies have reported that absolute lymphocyte count at the time of ATG administration could predict survivals in ASCT from unrelated donors. Here this issue was examined in 116 patients receiving peripheral blood stem cells (PBSC) ASCT with purine analog/busulfan-based conditioning regimens between 2009 and 2019 in our department. The impact of lymphopenia at the time of ATG administration was evaluated in terms of overall survival, disease-free survival and GVHD-free/relapse-free survival. After a median follow-up of 4 years, no adverse effect of a profound lymphopenia was observed on patients’ outcome. Notably, a reduced dose of ATG in patients with profound lymphopenia did not translate into better survivals. This study indicates that ATG can be administered whatever the recipient’s lymphocyte counts in patients receiving a PBSC purine analog/busulfan-based conditioning regimen ASCT.
Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones
Authors: Liang Yan-Fei , Yan Le-Tian , Yue Qiao , Zhao Ji-Kui , Luo Cai-Yun , Gao Feng , Li Heng , Gao Wen-Yun 
Published: September 2020

AbstractThe large catalytic subunit of acetohydroxyacid synthase (AHAS, EC 2.2.1.6) of Thermotoga maritima (TmcAHAS) was prepared in this study. It possesses high specific activity and excellent stability. The protein and a whole cell catalyst overexpressing the protein were applied to the preparation of α-hydroxyketones including acetoin (AC), 3-hydroxy-2-pentanone (HP), and (R)-phenylacetylcarbinol (R-PAC). The results show that AC and HP could be produced in high yields (84% and 62%, respectively), while R-PAC could be synthesized in a high yield (about 78%) with an R/S ratio of 9:1. Therefore, TmcAHAS and the whole cell catalyst overexpressing the protein could be practically useful bio-catalysts in the preparation of α-hydroxyketones including AC, HP, and R-PAC. To the best of our knowledge, this is the first time that bacterial AHAS was used as a catalyst to prepare HP with a good yield, and also the first time that TmcAHAS was employed to synthesize AC and R-PAC.
Towards developing robust solid lubricant operable in multifarious environments
Authors: Ayyagari Aditya V. , Mutyala Kalyan C. , Sumant Anirudha V. 
Published: September 2020

AbstractConventional solid lubricants such as MoS2, graphite, or diamond-like carbon films demonstrate excellent tribological performance but only in specific environments due to their inherent materials properties. This limitation prohibits using these solid lubricants in environments that change dynamically. This study presents the results of a novel solid lubricant that was developed using a combination of solution-processed 2D-molybdenum disulfide and graphene-oxide (GO) that can be deposited on to stainless steel substrates using a simple spray-coating technique and show exceptional performance in multifarious environments namely, ambient (humid) atmosphere, dry nitrogen, and vacuum. The tribological performance of the coatings was evaluated using a ball-on-disc sliding test and demonstrated an excellent wear/friction performance in all environments and coating survived even after 44 km of linear sliding. Transmission electron microscopy and Raman spectroscopy analysis of the tribolayers suggested in-operando friction-induced re-orientation of MoS2 layers that were protected by GO layers and, an absence of MoOx peaks indicate a strong resistance to intercalation with moisture and oxygen. The simplicity and robustness of the hybrid MoS2–GO solid lubricant in mitigating wear-friction behavior of steel-on-steel tribopair in a multifarious environment is a game-changing and is promising for various applications.
DNA minor-groove binder Hoechst 33258 destabilizes base-pairing adjacent to its binding site
Authors: Zhang Xin-Xing , Brantley Shelby L. , Corcelli Steven A. , Tokmakoff Andrei 
Published: September 2020

AbstractUnderstanding the dynamic interactions of ligands to DNA is important in DNA-based nanotechnologies. By structurally tracking the dissociation of Hoechst 33258-bound DNA (d(CGCAAATTTGCG)2) complex (H-DNA) with T-jump 2D-IR spectroscopy, the ligand is found to strongly disturb the stability of the three C:G base pairs adjacent to A:T the binding site, with the broken base pairs being more than triple at 100 ns. The strong stabilization effect of the ligand on DNA duplex makes this observation quite striking, which dramatically increases the melting temperature and dissociation time. MD simulations demonstrate an important role of hydration water and counter cations in maintaining the separation of terminal base pairs. The hydrogen bonds between the ligand and thymine carbonyls are crucial in stabilizing H-DNA, whose breaking signal appearing prior to the complete dissociation. Thermodynamic analysis informs us that H-DNA association is a concerted process, where H cooperates with DNA single strands in forming H-DNA.
The Milky Way Cepheid Leavitt law based on Gaia DR2 parallaxes of companion stars and host open cluster populations
Authors: Breuval Louise , Kervella Pierre , Anderson Richard I. , Riess Adam G. , Arenou Frédéric , Trahin Boris , Mérand Antoine , Gallenne Alexandre , Gieren Wolfgang , Storm Jesper , Bono Giuseppe , Pietrzyński Grzegorz , Nardetto Nicolas , Javanmardi Behnam , Hocdé Vincent 
Published: September 2020

Aims. Classical Cepheids provide the foundation for the empirical extragalactic distance ladder. Milky Way Cepheids are the only stars in this class accessible to trigonometric parallax measurements. However, the parallaxes of Cepheids from the second Gaia data release (GDR2) are affected by systematics because of the absence of chromaticity correction, and occasionally by saturation.
Methods. As a proxy for the parallaxes of 36 Galactic Cepheids, we adopt either the GDR2 parallaxes of their spatially resolved companions or the GDR2 parallax of their host open cluster. This novel approach allows us to bypass the systematics on the GDR2 Cepheids parallaxes that is induced by saturation and variability. We adopt a GDR2 parallax zero-point (ZP) of −0.046 mas with an uncertainty of 0.015 mas that covers most of the recent estimates.
Results. We present new Galactic calibrations of the Leavitt law in the V, J, H, KS, and Wesenheit WH bands. We compare our results with previous calibrations based on non-Gaia measurements and compute a revised value for the Hubble constant anchored to Milky Way Cepheids.
Conclusions. From an initial Hubble constant of 76.18 ± 2.37 km s−1 Mpc−1 based on parallax measurements without Gaia, we derive a revised value by adopting companion and average cluster parallaxes in place of direct Cepheid parallaxes, and we find H0 = 72.8 ± 1.9 (statistical + systematics) ±1.9 (ZP) km s−1 Mpc−1 when all Cepheids are considered and H0 = 73.0 ± 1.9 (statistical + systematics) ±1.9 (ZP) km s−1 Mpc−1 for fundamental mode pulsators only.
Effect of coronal loop structure on wave heating through phase mixing
Authors: Pagano P. , De Moortel I. , Morton R. J. 
Published: September 2020

Context. The mechanism(s) behind coronal heating still elude(s) direct observation and modelling of viable theoretical processes and the subsequent effect on coronal structures is one of the key tools available to assess possible heating mechanisms. Wave heating via the phase mixing of magnetohydrodynamic (MHD) transverse waves has been proposed as a possible way to convert magnetic energy into thermal energy, but MHD models increasingly suggest this is not an efficient enough mechanism.
Aims. We modelled heating by phase mixing transverse MHD waves in various configurations in order to investigate whether certain circumstances can enhance the heating sufficiently to sustain the million degree solar corona and to assess the impact of the propagation and phase mixing of transverse MHD waves on the structure of the boundary shell of coronal loops.
Methods. We used 3D MHD simulations of a pre-existing density enhancement in a magnetised medium and a boundary driver to trigger the propagation of transverse waves with the same power spectrum as measured by the Coronal Multi-Channel Polarimeter. We consider different density structures, boundary conditions at the non-drive footpoint, characteristics of the driver, and different forms of magnetic resistivity.
Results. We find that different initial density structures significantly affect the evolution of the boundary shell and that some driver configurations can enhance the heating generated from the dissipation of the MHD waves. In particular, drivers coherent on a larger spatial scale and higher dissipation coefficients can generate significant heating, although it is still insufficient to balance the radiative losses in this setup.
Conclusions. We conclude that while phase mixing of transverse MHD waves is unlikely to sustain the thermal structure of the corona, there are configurations that allow for an enhanced efficiency of this mechanism. We provide possible signatures to identify the presence of such configurations, such as the location of where the heating is deposited along the coronal loop.
Coinfection of HIV and hepatitis C increases complication rates after total joint arthroplasty
Authors: Fang Christopher , Cornell Ella , Dicken Quinten , Freccero David , Mattingly David , Smith Eric L. 
Published: September 2020

Introduction: As advances in efficacy of human immunodeficiency virus (HIV) and hepatitis-C virus (HCV) anti-viral medications increase, patients are able to maintain higher quality of lives than ever before. While these patients live longer lives, the unique patient population of those co-infected with both HIV and HCV increases. As these older patients seek orthopaedic care, it is important to understand their unique outcome profile. The purpose of this study was to evaluate the complication rate after total joint arthroplasty (TJA) in patients with HIV and HCV coinfection compared with patients with HIV or HCV only. Methods: A retrospective review of patients undergoing primary total joint arthroplasty (TJA) at our urban, academic hospital between April 2016 and April 2019 was conducted. Patients were stratified into three groups according to viral status: HIV only, HCV only, or HIV and HCV coinfection. Baseline demographics, intravenous drug (IV) use, surgery type, CD4+ count, follow-up and complications were analysed. Results: Of the 133 patients included in the study, 28 had HIV, 88 had HCV and 17 were coinfected with both HIV and HCV. Coinfected patients were more likely to have a lower BMI (p < 0.039) and a history of IV drug use (p < 0.018) compared to patients with either HIV or HCV only. Coinfected patients had a higher complication rate (41%) than both HIV only (7%; p < 0.001) and HCV only (12.5%; p < 0.001) patients. Discussion: Patients coinfected with HIV and HCV undergoing TJA have a higher complication rate than patients with either infection alone. As this unique population of coinfected patients continues to expand, increasingly they will be under the care of arthroplasty surgeons. Improved awareness and understanding of the baseline demographic differences between these patients is paramount. Recognition of the increased complication rates grants the opportunity to improve their orthopaedic care through preoperative and multidisciplinary management.
A novel technique for mapping biopsy of bile duct cancer
Authors: Okada Hirokazu , Uza Norimitsu , Matsumori Tomoaki , Matsumoto Shimpei , Muramoto Yuya , Ota Sakiko , Nakamura Takeharu , Yoshida Hiroyuki , Hirano Tomonori , Kuwada Takeshi , Marui Saiko , Sogabe Yuko , Morita Toshihiro , Kakiuchi Nobuyuki , Mima Atsushi , Ueda Tatsuki , Nishikawa Yoshihiro , Tsuda Motoyuki , Maruno Takahisa , Shiokawa Masahiro , Takahashi Ken , Taura Kojiro , Minamiguchi Sachiko , Kodama Yuzo , Seno Hiroshi 
Published: September 2020

Abstract
Background Accurate preoperative assessment of the longitudinal extension of perihilar cholangiocarcinoma (PHCC) is essential for treatment planning. Mapping biopsies for PHCC remain challenging owing to technical difficulties and insufficient sample amounts. The aim of this study was to investigate the usefulness of a novel technique for mapping biopsies of PHCC.
Methods Our novel method focused on a biliary stent delivery system for mapping biopsies. Fifty patients with PHCC undergoing endoscopic transpapillary mapping biopsy using the novel method were reviewed from August 2015 to June 2019.
Results The median number of biopsy samples was six (range 1 – 17), and the rate of adequate sampling was 91.4 % (266 /291). Biopsy from the intrahepatic bile duct was possible in 82.0 % of patients (41 /50), and negative margins were confirmed in the resected specimens from 34 /39 patients who underwent surgery (87.2 %). None of the patients had post-endoscopic retrograde cholangiopancreatography pancreatitis.
Conclusions With our novel method, accurate assessment of the longitudinal extension of PHCC might be expected with minimal trauma to the duodenal papilla.