bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acupuncture of fascia points to relieve hand spasm after stroke: a study protocol for a multicenter randomized controlled trial
Authors: zhang zengqiao , Feng Wei , Li Kun-Peng , He Jing , Jiang Li-Ming , Wang Wu , Hu Xiao-Shen 
Published: November 2019

Abstract
Background: The loss of life ability of patients after stroke is mostly caused by the dysfunction of upper limbs, especially hands. Hand functional exercise is the premise of alleviating hand dysfunction, and the relief of hand spasm is the basis of timely and effective hand functional exercise. Previous clinical observation showed that fascial point needling could effectively alleviate hand spasm immediately after stroke, but further evidence from large sample studies is needed. The overall objective of this trial is to further evaluate the clinical efficacy of fascial point acupuncture on hand spasm after stroke. Methods/design: This multicenter randomized controlled trial will compare the efficacy of fascial point acupuncture versus sham acupuncture and routine rehabilitation therapy in stroke patients with hand spasm. Patients will be randomized to undergo either the fascial point acupuncture or the sham acupuncture or the control (routine rehabilitation therapy). We will recruit 210 stroke inpatients who meet the trial criteria and observe the remission of hand spasm and improvement of limb function after 4 weeks of intervention. The first evaluation indexes are the remission of hand spasm and the duration of spasm remission. The second evaluation indexes are the hand function of affected limbs and the ability of daily living. When the accumulative total number of cases included reaches 120, a mid-term analysis will be conducted to determine any evidence that experimental intervention does have an advantage. Discussion: Our aim is to evaluate the efficacy of fascial point acupuncture in relieving hand spasm after stroke. The results will provide more evidences for the clinical application of this therapy in the future. Trial registration: The trial has been registered at the Chinese Clinical Trial Registry（ChiCTR）on April 9, 2019. Registration number: ChiCTR1900022379 Keywords: Fascia points, Acupuncture, Spasm, Stroke
Population-level effects of diet, lifestyle, and health environment on obesity and non-communicable diseases in India
Authors: Joshi Kuhu , Roy Devesh , Iannotti Lora , Nagar Aishwarya , Kishore Avinash 
Published: November 2019

Abstract
Background: Obesity is rising in developing countries like India and is associated with an increase in cardiometabolic problems. Rising incomes, rapid urbanization, and mechanization have induced lifestyle changes like consumption of more obesogenic foods and sedentary habits at work and leisure, contributing to a transition from under- to over-nutrition. This study maps the prevalence of adult (15-49 years) overweight and obesity across regions and socioeconomic groups in India, and estimates its association with lifestyle, health environment, dietary patterns, diabetes, and hypertension.Methods: We employ a combination of 3 latest nationally representative datasets with over 700,000 adults. We use a linear probability regression model to identify the correlates of overweight/obesity and their relative magnitudes. We use intra-household regression to identify differences between men and women and coarsened exact matching to causally estimate the impact of obesity on diabetes and hypertension.Results: Overweight/obesity rates have increased across all states, in rural and urban areas, and for all wealth levels. Women are more likely to be overweight/obese than men, even in the same household. Improved health environment (toilets, piped water, clean cooking fuel), urban jobs, television watching, and processed snacks increase the risk of overweight/obesity. Adults who are overweight/obese have a 5.6% higher risk of diabetes and a 9.7% higher risk of hypertension.Conclusions: Our results underscore the need for policy intervention to reduce the burden of obesity and NCD’s in India.
Incidence Of Diarrheal Infections In Ukraine: Epidemiological Peculiarities
Authors: Malysh Nina , Chemych Oksana , Rodyna Roman , Chorna Inna , Doan Svitlana 
Published: November 2019

Abstract
Background : Diarrheal infections remain relevant for many countries of the world. The processes of globalization, fundamental changes in nutrition and water consumption contributed to the fact that the significance of individual infection sources, etiological structure of diarrheal infections changed. Purpose of the study: on the background of the analytical study of the incidence of diarrheal infections in Ukraine under the current conditions, to determine the factors influencing epidemic situation. Methods: The reports of the Ministry of Health of Ukraine, Main Administrations of Statistics in Kharkiv, Odesa, Zaporizhia oblasts for 2011-2018 are used in the paper. Epidemiological and statistical research methods are applied. Results: The epidemic situation with diarrheal infections in Ukraine is characterized by a low incidence of typhoid fever (from 0.012 to 0.14 per 100 thousand people), shigellosis (from 1.97-6.13 per 100 thousand people), stable incidence rates without the downward trend, salmonellosis (from 17.35 till 24.1 per 100 thousand people), high incidence of diahrreal infections of specified etiology (from 115.5 to141.9 per 100 thousand people) and diahrreal infections of unspecified etiology (from 69.76 to 107.02 per 100 thousand people). The most complicated epidemic situation is observed in economically most developed regions of the country. Most diarrheal infectionsoutbreaks are connected with catering establishments 36.5 % and with children educational establishments 26.1 %. In the region with the highest shigellosis and salmonellosis incidence direct strong correlation relationships are established between the incidence and population, density, natural population movement. The oblasts with the highest diahrreal infections of specified etiology, rotaviral enteritis, diahrreal infections of unspecified etiology incidence are at least provided with water resources and have problems with provision of high-quality drinking water. There is a need to improve the system of epidemiological surveillance over diarrheal infections by extension of the indicators of microbiological study of drinking water quality in the regions of Ukraine, where high diahrreal infections of specified etiology, rotaviral enteritis, diahrreal infections of unspecified etiology incidence is registered; by increasing frequency of food item inspections in the oblasts, where mediana shigellosis and salmonellosis incidence exceeds the average one in Ukraine. Keywords: diarrheal infections, shigellosis, salmonellosis, rotaviral enteritis.
Genomic and transcriptomic survey of an endophytic fungus Calcarisporium arbuscula NRRL 3705 and potential overview of its secondary metabolites
Authors: Cheng Jintao , Cao Fei , Chen Xinai , Li Yongquan , Mao Xuming 
Published: November 2019

Abstract
Endophytic fungi can produce many active secondary metabolites, which are important resources of natural medicines. However, there is currently little understanding of endophytic fungi at the omics levels. Calcarisporium arbuscula , an endophytic fungus from the healthy fruit of russulaceae, can produce a variety of secondary metabolites with anti-cancer, anti-nematode and antibiotic bioactivities. Comprehensive survey of the endophytic fungi genome and transcriptome will help to understand their capacity to biosynthesize secondary metabolites and lay the foundation for the development of these precious resources. In this study，we reported the high-quality genome sequence of a strain C. arbuscula NRRL 3705 based on Single Molecule Real-Time sequencing technology. The genome of this fungus is over 45 Mb in size, relatively larger than other typical filamentous fungi, and comprises 10,001 predictable genes, encoding at least 762 secretory-proteins, 386 carbohydrate-active enzymes and 177 P450 enzymes. 398 virulence factors and 228 genes related to pathogen-host interactions were also predicted in this fungus. Moreover , 65 secondary metabolite biosynthetic gene clusters were revealed, including the gene cluster for mycotoxins aurovertins. In addition, several gene clusters were predicted to produce various mycotoxins, including aflatoxin, alternariol, destruxin, citrinin and isoflavipucine. Notably, two independent gene clusters were shown possibly involved in the biosynthesis of alternariol. Furthermore, RNA-Seq assay showed that only the expression of aurovertin gene cluster is much stronger than the housekeeping genes under laboratory conditions, consistent with that aurovertins are the predominant metabolites. The gene expression of the remaining 64 gene clusters for compound backbone biosynthesis was all lower than the housekeeping genes, which might partially explain poor production of other secondary metabolites in this fungus.Our omics data along with bioinformatics analysis indicated that C. arbuscula NRRL 3705 contains a large number of biosynthetic gene clusters and has a huge potential to produce profound secondary metabolites. This work also provides the basis for development of endophytic fungi as a new resource of natural products with promising biological activities.
Impact of Non-synchronous Diagnostic Ureteroscopy on Intravesical Recurrence in Patients Underwent Radical Nephroureterectomy for Upper Tract Urothelial Carcinoma: A Propensity-Score Matched Analysis
Authors: Ye Huamao , Feng Xiang , Wang Yang , Chen Rui , Hua Meimian , Lu Xiaojun , Jiang Xiaoqin , Fang Yu , Wu Zhenjie , Yang Qing , Yang Bo , Lü Chen , Wang Linhui 
Published: November 2019

Abstract
Background: The effect of diagnostic ureteroscopy (DURS) on intravesical recurrence (IVR) after radical nephroureterectomy (RNU) were controversial. To investigate the impact of DURS, we carried out this single-center retrospective study by applying propensity-score matching (PSM) and Cox regression model. Patients and Methods: The data of 160 patients with pTa-pT3 upper tract urothelial carcinoma (UTUC) were analyzed. Eighty-six patients underwent DURS (DURS group) and 74 patients without DURS (control group). The DURS group was further sub-grouped into synchronous DURS group (DURS followed by immediate RNU, n=45) and non-synchronous DURS group (DURS followed by delayed RNU, n=41). Baseline confounders were corrected by PSM. The impact of DURS on IVR was assessed by Kaplan-Meier analysis in PSM cohort and by Cox regression model in the full data set. Results: The median follow-up time was 40.4 months. No difference of the 3-year IVRFS between DURS group and control group (72.6% vs. 65.3%, p=0.263). In subgroup analysis, the 3-year IVR-free survival of non-synchronous DURS group (51.4%) was significantly lower than that of synchronous DURS (78.3%) or control group (72.6%) (p=0.027). Further Cox regression analysis showed that non-synchronous DURS (HR 1.481, 95% CI 1.031-2.127, p=0.034) was independent risk factors for postoperative IVR. Conclusions: Non-synchronous DURS was not recommended for the diagnosis and preoperative evaluation of UTUC, because it could raise the risk of IVR after RNU. For UTUC patients in need of DURS, synchronous DURS could be a safer choice than the non-synchronous DURS in terms of lowering the IVR risk.
The prognostic role of Controlling Nutritional Status (CONUT) scores in patients with gastrointestinal stromal tumors
Authors: Yang Weili , Shou Chunhui , Yu Jiren , Zhang Qing , Liu Xiaosun , Yu Hang , Lin Xianke 
Published: November 2019

Abstract
Background: The Controlling Nutritional Status (CONUT) score is associated with the postoperative outcomes in various types of tumors. The relationship between the CONUT score and prognosis in patients with gastrointestinal stromal tumors (GISTs) needs to be clarified.
Methods: Patients with completely resected primary GISTs in the absence of imatinib adjuvant therapy were included. Recurrence-free survival (RFS) was estimated with the Kaplan-Meier method and compared using log-rank test. Prognostic factors were compared using a Cox proportional hazards model.
Results: A total of 455 patients were included. The median age was 57 years and 222 (48.8%) patients were male. The most common location was stomach (n= 219, 48.1%), the median tumor size was 4.5 cm (range 0.4-40.0) and the median mitotic index was 2/50 HPFs (range 0-200). Recurrence/metastasis developed in 92 (20.2%) patients. Patients were assigned to three groups: 219 (48.1%) were in normal nutrition group (CONUT=0-1), 196 (43.1%) were in light undernutrition group (CONUT=2-4) and 40 (8.8%) were in moderate-severe undernutrition group (CONUT≥5). Primary tumor site, tumor size, mitotic index, tumor rupture and CONUT score were independent prognostic factors for RFS using multivariate analysis (p<0.05).
Conclusions: The CONUT score was an independent prognostic factor for patients with completely resected GIST.
Prognostic value and clinicopathological characteristics of PD-L1 overexpression in non-Hodgkin lymphoma: a meta-analysis
Authors: Zeng Qiang , Liu Zhigang , Liu Ting 
Published: November 2019

Abstract
Abstract
Background: Programmed cell death ligand 1 (PD-L1) has already been detected in various carcinomas. In non-Hodgkin lymphoma (NHL), however, the prognostic value of PD-L1 overexpression remains unclear.
Methods: A meta-analysis of 2,321 NHL patients from 12 studies was performed. HRs with 95% CIs were used to evaluate the correlation between PD-L1 overexpression and prognosis of NHL, and odds ratios (ORs) with 95% CIs were used to assess the association of PD-L1 overexpression with clinicopathological factors.
Results: The result showed that no significant difference between PD-L1 positive and negative groups was detected in NHL (hazard ratio [HR]: 1.40, 95% confidence interval [CI]: 0.90-2.19; P =0.137). Nevertheless, the result indicated that PD-L1 overexpression was associated with poor prognosis in the subtype of diffuse large B cell lymphoma (DLBCL) (HR: 1.70, 95%CI: 1.05-2.74; P =0.031). We also performed subgroup analyses and meta-regression. The pooled odds ratios（ORs） showed that PD-L1 overexpression was associated with B symptoms, higher international prognostic index (IPI) score (3, 4 and 5 points) and Ann Arbor Stage III and IV.
Conclusions: T he meta-analysis demonstrated that PD-L1 expression was not associated with prognosis of NHL but associated with prognosis of DLBCL.
Key words: PD-L1, non-Hodgkin lymphoma, prognosis, meta-analysis
Resting Energy Expenditure Relationship with Macronutrients and Gestational Weight Gain: A Pilot Study
Authors: Wyst Kiley B. Vander , Buman Matthew P. , Shaibi Gabriel Q. , Petrov Megan E. , Reifsnider Elizabeth , Whisner Corrie M 
Published: November 2019

Abstract
Background: Resting energy expenditure (REE) comprises 60% of total energy expenditure and variations may be associated with gestational weight gain (GWG) or maternal diet. The objective of this study was to examine the impact of ambulatory metabolic tracking on GWG and the association with maternal macronutrient composition during mid-pregnancy.
Methods: Pregnant women aged 29.8±4.9 yr (78.6% non-Hispanic, White) with gestational ages (GA) < 17 wk were randomized to Breezing™ (N=16) or control (N=12) groups for 13 wks. REE by Breezing™ indirect calorimetry, anthropometrics and dietary intake were collected every two weeks. Early (GA weeks 14-21), late (GA weeks 21-28), and overall (GA week 14-28) changes in macronutrients and GWG were calculated.
Results: Overall total and rate of GWG did not differ by group but Breezing™ women had lower rate and total GWG in the beginning of the second trimester but gained significantly more weight [F(1,24)=5.29, p=0.03] in the latter half. Independent of group assignment, early increases in REE were associated with an increased rate of GWG later in pregnancy (r=0.53, p=0.05). Dietary intake did not differ by group at baseline; however, overall energy (-144±339 vs. 192±422, p=0.03) and protein (-10±24 vs. 13±31, p=0.05) intake were significantly lower for the Breezing than the control women which primarily occurred in the latter half of the second trimester.
Conclusion: Regular metabolism tracking alone did not have an impact on GWG. Assessment of REE during pregnancy in conjunction with nutritional counseling or education may result in improvements in GWG.
Prognostic role of time to positivity of blood culture in children with Pseudomonas aeruginosa bacteremia.
Authors: xu huiting , Cheng Jie , Yu Qinghong , Li Qingyuan , Yi Qian , Luo Siying , Li Yuanyuan , Zhang Guangli , Tian Xiaoyin , Cheng Dapeng , Luo Zhengxiu 
Published: November 2019

Abstract
Background

Pseudomonas aeruginosa ( P. aeruginosa ) is a major Gram-negative pathogen, which has been reported to result in high mortality. We aim to investigate the prognostic value and optimum cut-off point of time-to-positivity (TTP) of blood culture in children with P. aeruginosa bacteremia.Methods

From August 2014 to November 2018, we enrolled the inpatients with P. aeruginosa bacteremia in a 1500-bed tertiary teaching hospital in Chongqing, China retrospectively. 52 case were analyzed. Receiver operating characteristic (ROC) analysis was used to determine the optimum cut-off point of TTP, and logistic regression were employed to explore the risk factors for in-hospital mortality and sepsis shock.Results

Totally, 52 children with P. aeruginosa bacteremia were enrolled. The standard cut-off point of TTP was18 hours. Early TTP (≤18 hours) group patients had remarkably higher in-hospital mortality (42.9% vs 9.7%, P=0.014), higher incidence of sepsis shock (52.4% vs 12.9%, P=0.06), higher Pitt bacteremia scores [3.00 (1.00-5.00) vs 1.00 (1.00-4.00), P=0.046] and more intensive care unit admission (61.9% vs 22.6%, P=0.008) when compared with late TTP (>18 hours) groups. Multivariate analysis indicated TTP ≤18 h, Pitt bacteremia scores ≥4 were the independent risk factors for in-hospital mortality (OR 5.88, 95%CI 1.21-21.96, P=0.035; OR 4.95, 95%CI 1.26-27.50, P=0.024; respectively). The study also revealed that the independent risk factors for sepsis shock were as follows: TTP≤18h, Pitt bacteremia scores≥4 and hypoalbuminemia (OR 6.30, 95%CI 1.18-33.77, P=0.032; OR 8.15, 95%CI 1.15-42.43, P=0.014; OR 6.46, 95% CI 1.19-33.19 P=0.031; respectively).Conclusions

Early TTP (≤18 hours) appeared to be associated with worse outcomes for P. aeruginosa bacteremia children.
CHANGES IN DNA METHYLATION PROFILE IN TAMOXIFEN-RESISTANT MCF-7 SUBLINES
Authors: Andreeva О. Е. , Sigin V. О. , Strelnikov V. V. , Tanas A. S. , Scherbakov A. M. , Krasilnikov M. A. 
Published: November 2019

Abstract Introduction. We have previously shown the feasibility of hormonal resistance horizontal distribution from cell to cell, with the joint cultivation of sensitive and resistant cells and/or through exosomes secreted by resistant cells. What is the mechanism of such resistance distribution, and how do cells with secondary resistance reproduce the characteristics of donor resistant cells? To answer these questions, we analyzed the overall level of DNA methylation in MCF-7 estrogen-dependent breast cancer cells and estrogen-independent sublinia.The purpose of the study was to analyze DNA methylation profiles for the development of hormonal resistance by breast cancer cells and for resistant phenotype further accession.Methods. DNA methylation was evaluated by the RRBS (Reduced Representation Bisulfite Sequencing) method in MCF-7 breast cancer cells and their resistant sublines.Results. 19 CpG dinucleotides, differentially and generally unidirectionally methylated in cells with primary and secondary resistance to tamoxifen, were detected. Differential changes in methylation were found for DNA regions that regulated the expression of six protein-coding genes: PRKCZ, TRAPPC9, AS IC2, C2CD4A, ZNF787, CRTAC 1. Bioinformatics analysis showed that two of these six genes, PRKCZ (protein kinase C Zeta) and TRAPPC9 (Trafficking Protein Particle Complex Subunit 9) were directly involved in the regulation of NF-κB activity.Conclusion. The data obtained indicate the existence of common DNA patterns, the methylation of which varies in the same direction in cells with primary and secondary resistance. The involvement of two of the identified genes in the regulation of NF-κB may indicate the inclusion of the latter in the formation of a resistant phenotype of tumor cells, even under conditions of horizontal transfer of resistance.
A COMPARISON OF THE CANCER INCIDENCE RATES BETWEEN THE STATE CANCER STATISTICS AND CANCER REGISTRY DATA IN THE RUSSIAN FEDERATION
Authors: Petrova G. V. , Gretsova O. P. , Starinsky V. V. 
Published: November 2019

The purpose of the study was to compare data on the cancer incidence rates for 2016 between the official reports on cancer statistics and federal cancer registry, collected in December 2018.Material and Methods. The study estimated the total data on 18 parameters from 35 regions of Russia, covering 66.3 million people (2016). The database of the Russian cancer registry and the database containing reports on the state cancer statistics were used. The cancer statistics/cancer registry ratio was assessed.Results. No differences in cancer incidence between the official reports on cancer statistics and cancer registry data were found. In the official reports on cancer statistics, the mortality rate, the proportion of posthumously recorded patients per 100 newly diagnosed, the proportion of deaths from diseases not related to cancer per 100 deceased patients, the cancer prevalence and the prevalence rate of unspecified malignant tumors were slightly reduced (to 10 %, 9 %, 5 %, and 4 %, respectively), and the rate of cancer detection, the proportion of histologically verified diagnoses and the proportion of cancers detected in stage III were increased (to 19 %, 10 % and 14 %, respectively) compared to those in cancer registry data.Conclusion. Improvement in the quality and completeness of information about cancer patients is associated rather with increasing the annual report length than with the need to improve the cancer registration system itself.
Health and Safety Perception of Construction Workers in Saudi Arabia
Authors: SANNI-ANIBIRE Muizz O. , MAHMOUD Abubakar S. , HASSANAIN Mohammad A. , ALMUTAIRI Faleh 
Published: November 2019

Abstract
Construction is a high-risk industry due to the high rates of accidents/fatalities recorded annually in various countries. Health and safety (HS) is a major concern of the construction industry. More so, for developing countries where HS cultures are yet to become fully established. In such situations, the perception of construction workers is a viable approach to assess the performance of HS. This study investigated the HS conditions in Saudi Arabian construction sites from the perspective of construction labor. The study employed a questionnaire comprised of 50 issues related to safety and health from an employee’s perspective, on a 1-5 Likert scale of agreement. Responses were obtained from 196 construction workers in the Eastern Province of Saudi Arabia. Results from the study showed that the crucial issues identified include: lack of communication between front-line supervisors and construction labor, less priority for safety compared to productivity; lack of employee-engagement in developing safety policies; poor morale and lack of motivation amongst workers; and un-tested emergency response procedures. Thus, the study suggests that addressing such issues will allow for improved safety performance in the Saudi Arabian construction industry.