bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the SureX HPV Genotyping Test for the Detection of High-Risk HPV in Cervical Cancer Screening
Authors: Wei Baojun , Mei Ping , Huang Shengkai , Yu Xueting , Zhi Tong , Wang Guojing , Xu Xiaotian , Xiao Lin , Dong Xin , Cui Wei 
Published: June 2020

Abstract
Background: The SureX HPV genotyping test (SureX HPV test), which targets the human papillomavirus (HPV) E6/E7 genes was compared with the Cobas 4800 and Venus HPV tests for detecting 14 high-risk HPV (HR-HPV) types in clinical referral and follow-up patients to evaluate its value for cervical cancer screening.Methods: Two different populations were enrolled in the study. The first population comprised 185 cases and was used for comparing the SureX HPV test (Health, China) with the Cobas 4800 test (Roche, USA). The second population comprised 290 cases and was used for comparing the SureX HPV test (Health, China) with the Venus HPV test (Zhijiang, China). Polymerase chain reaction (PCR) sequencing was performed for further confirmation of discordant results.Results: In the first population, the overall agreement rate was 95.3% for 14 High-Risk HPV types. Eight discordant cases were confirmed by PCR sequencing, which showed that the agreement rates were 75.0% between the SureX HPV test and PCR sequencing and 25.0% between the Cobas 4800 test and PCR sequencing (P<0.01). In the second population, the overall agreement rate was 94.5%. Thirteen discordant cases were confirmed by PCR sequencing, which showed that the agreement rates were 76.9% between the SureX HPV test and PCR sequencing and 23.1% between the Venus HPV test and PCR sequencing (P<0.01). With cervical intraepithelial neoplasia grade 2+ (CIN2+) as the reference standard, the sensitivity values of the SureX HPV test and the Venus HPV test were 93.5% and 92.0%, (P>0.05), while the specificity values were 43.3% and 46.7%, respectively (P>0.05).Conclusion: The SureX HPV test had good consistency with both the Cobas 4800 and Venus HPV tests for 14 HR-HPV types. In addition, it avoided some false negatives and false positives. Therefore, the SureX HPV test can be used for cervical cancer screening.
From dissociative experience and internet use to happiness: A Taiwan Birth Cohort Study
Authors: Lung For-Wey , Shu Bih-Ching , Chiang Tung-Liang , Lin Shio-Jean 
Published: June 2020

Abstract
Background

Functional and excessive use of internet is hard to distinguish, and internet use can affect adolescents’ development of self-identity. The aim of our study was to investigate the pathway relationship of the risk and protective factors of internet use, including mother care, absorptive dissociative trait, having been bullied, exercise, self-perceived depressive mood and happiness of 12-year-old adolescents.
Methods

The Taiwan Birth Cohort Study dataset, which used a national household probability sampling method and included 17,694 12-years-old adolescents, was used for this study.
Results

Our results showed 5.3% adolescents reported to spend more than five hours online during schooldays. Additionally, adolescents that spend more than five hours online during schooldays tended to have higher absorptive trait, perceived less care from mothers, more likely to have been bullied, expressed higher level of depressed mood, which leads to lower level of perceived happiness.
Conclusions

Adolescents that spend more than 5 hours online during schooldays, compared to those that spent less than an hour online, were more likely to have been bullied and affect their level of happiness, showing that they may be a group of higher concern. Since parental care and regular exercise are protective factors for internet addiction, it should be promoted to all adolescents, especially those in the high risk group, to prevent them from being addicted online.
Gut Microbiota as Prognosis Markers for Patients with HBV-Related Acute-on-Chronic Liver Failure
Authors: Wang Ke , Zhang Zhao , Mo Zhi-Shuo , Yang Xiao-Hua , Lin Bing-Liang , Pen Liang , Xu Yang , Lei Chun-Yan , Zhuang Xiao-Dong , Lv Ling , Yang Rui-Fu , Chen Tao , gao zhi-liang 
Published: June 2020

Abstract
Background
The gut microbiota in the hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF) is poorly defined. We aim to uncover the characteristics of the gut microbiota in HBV-ACLF and in other HBV associated pathologies.
Methods
We analyzed the gut microbiome in patients with HBV-ACLF or other HBV associated pathologies and healthy individuals by 16S rRNA sequencing and metagenomic sequencing of fecal samples. 212 patients with HBV-ACLF, 252 with chronic hepatitis B (CHB), 162 with HBV-associated cirrhosis (HBV-LC) and 877 healthy individuals were recruited for the study. CHB and HBV-LC patients are grouped as HBV-Other.
Results
We discovered striking differences in the microbiome diversity between the HBV-ACLF, HBV-Other and healthy groups using 16S rRNA sequencing. The ratio of cocci to bacilli was significantly elevated in the HBV-ACLF group compared with healthy group. Further analysis within the HBV-ACLF group identified 52 genera showing distinct richness within the group where Enterococcus was enriched in the progression group whilst Faecalibacterium was enriched in the regression group. Metagenomic sequencing validated these findings and further uncovered an enrichment of Lactobacillus casei paracasei in progression group, while Alistipes senegalensis, Faecalibacterium prausnitzii and Parabacteroides merdae dominated the regression group. Importantly, our analysis revealed that there was a rapid increase of Enterococcus faecium during the progression of HBV-ACLF.
Conclusions
The gut microbiota displayed distinct composition at different phases of HBV-ACLF. High abundance of Enterococcus is associated with progression while that of Faecalibacterium is associated with regression of HBV-ACLF. Therefore the microbiota features hold promising potential as prognostic markers for HBV-ACLF.
Finnish version of the Childbirth Experience Questionnaire (CEQ-FI): validity and reliability assessment
Authors: Toivonen Elli , Dencker Anna , Uotila Jukka , Huhtala Heini , Palomäki Outi 
Published: June 2020

Abstract
BackgroundChildbirth Experience Questionnaire (CEQ) was developed in Sweden to assess the childbirth experience in multiple dimensions. It has been translated and validated in English, Spanish, Chinese and Persian with the aim to evaluate childbirth experience reliably. This study aimed to validate the Finnish version of the questionnaire, CEQ-FI.MethodsPrimiparous women who had given birth in Tampere University Hospital between January and May 2019 were included in the study. Women planning a cesarean delivery, delivering preterm, or women whose children were transferred to neonatal care were excluded. Eligible 450 women were mailed the questionnaire one month postpartum, and those who completed the questionnaire were mailed it again six weeks postpartum. Test-retest reliability was evaluated by computing kappa coefficients for the two responses. Background data was collected from patient records and used to perform known-groups validation. Internal consistency was assessed by calculating Cronbach’s alpha.ResultsOne hundred seventy-five women returned the questionnaire. Internal consistency and test-retest reliability were good. Women with known risk factors for a negative childbirth experience scored lower in the questionnaire, as expected.ConclusionCEQ-FI is a valid and reliable instrument in measuring childbirth experience also among Finnish-speaking women.
Parent feedback: perception of parents and pediatric residents in Asian culture
Authors: Pakakasama Samart , Donkers Jeroen , Puranitee Pongtong 
Published: June 2020

Abstract
Background

Residency programs have used feedback of parents as a part of multisource feedback to improve residents’ skills. However, there is no evidence showing how parents can appropriately be engaged in pediatric resident feedback in our context. This study aimed to investigate: 1) the resident skills assessed by parents in perception of Thai parents and pediatric residents and 2) the parent characteristics making credible feedback in perception of Thai pediatric residents.
Methods

The participating parents were asked to rate their confident to assess residents’ skills according to 17 items of the PARENTS questionnaire (Likert scale 1–5). To enhance the reasons of parent’s confident, the parents responded to think aloud interviews. We conducted focus groups in order to explore the resident perception on parents’ assessable resident skills and credible parent characteristics.
Results

There were 51 parents revealing the mean confident scores of 17 items ranged from 4.06 to 4.37. The parents felt confident to assess the residents’ skills according to the questionnaire because they directly experienced the residents’ performance during admissions. Five resident focus groups were conducted. In resident perception, residents’ skills in communication, collaboration, leadership and professionalism were assessable by parents. The characteristics affecting the feedback credibility included parents’ prior experience, personal behavior and confrontation to medical situation.
Conclusions

Thai residency programs may use parent feedback for residents’ performance improvement as both parents and residents are able to identify the parents’ assessable skills. The feedback process should consider the credible parents’ characteristics to increase the acceptability and avoid the bias.
Prevalence and Molecular Spectrum of α- and β-Globin Gene Mutations in Hainan, China
Authors: Wang Zhen , Sun Wenye , Chen Huaye , Zhang Yongfang , Wang Fei , Huang Yanhua , Chen Hongjian , Zhou Yao , Zhou Xixi , Li Qi , Ma Yanlin 
Published: June 2020

Abstract
Background: Thalassemia is one of the most prevalent inherited single gene diseases. Prevention of β-thalassemia through prenatal diagnosis is the one of the most effective and direct approach to control the spread of this life-threatening disease. This study aims to determine the prenatal diagnosis of α-thalassemia and β-thalassemia in 3049 families among eighteen regions of Hainan Province using molecular diagnosis.Methods: This study enrolled a total of 3049 couples and their fetuses at The First Affiliated Hospital of Hainan Medical University from January 2004 to March 2020. Genomic DNA was extracted from peripheral blood of the couples and villus, amniotic fluid, or fetal cord blood of fetuses. DNA-based diagnosis was performed using polymerase chain reaction. Results: Here, the most commonly detected mutation of α-thalassemia was a South-East Asian deletion (31.53%), followed by –α4.2/αα (11.15%), –α3.7/αα (11.02%). The most common mutation for β-thalassemia was CD41/42, followed by -28, accounting for 30.27% and 2.56%, respectively. The regions with the highest prevalence were the coastal regions and the regions with the lowest prevalence were Wenchang, Lingao and Ding’an. We also examined thalassemia gene mutations in Han people and other minority groups and found that the most common gene mutations in different ethnic were not homogeneous. Prenatal diagnosis showed 556 normal, 118 α-thalassemia hydrops and 161 β-thalassemia major fetuses. Conclusion: Our findings provide important information for clinical genetic counseling of prenatal diagnosis for thalassemia major in Hainan Province.
LncRNA CEBPA-AS1 Alleviates Cerebral Ischemia-Reperfusion Injury by Sponging miR-340-5p to Regulate APPL1/LKB1/AMPK Pathway
Authors: Tu Xiankun , Zhang Huabin , Huang Bin , Wu Xiyao , Shi Songsheng 
Published: June 2020

Abstract
BACKGROUND: The aim of this study was to research the mechanism of lncRNA CEBPA-AS1 in cerebral ischemia-reperfusion injury (CIRI). METHODS: Middle cerebral artery occlusion (MCAO), MCAO/IR and OGD/R models were constructed. RNA immunoprecipitation (RIP) detected the binding of miR-340-5p to CEBPA-AS1. CEBPA-AS1 and miR-340-5p was knockdown or up-regulated. RT-PCR and western blot was processed to detect the expression of related genes and proteins. Brain tissue water content in each group was determined. Nissl staining in hippocampus of the brain and NeuN staining (green) assay was used to observe nerve damage and detect nerve cell survival, respectively. Cell viability was detected by MTT assay. Nerve cell survival was observed by immunofluorescence cytochemistry assay. LDH and MDA content were detected by kit. TUNEL was used to detect apoptosis. Luciferase reporter system was processed for verifying the binding sites. After overexpressing CEBPA-AS1 in PC-12 cells, RNA pulldown assay was performed. TTC assay was used to observe the general morphology of the brain. RESULTS: Overexpression of CEBPA-AS1 attenuated MCAO/IR-induced nerve damage. Increased CEBPA-AS1 expression reduced neuronal apoptosis in MCAO/IR model. Knockdown of CEBPA-AS1 aggravated cell damage OGD/R cell model. CEBPA-AS1 increased APPL1 expression via negatively regulating miR-340-5p, and affected APPL1/LKB1/AMPK pathway. CEBPA-AS1 attenuated OGD/R-induced cell damage by reducing miR-340-5p levels. CONCLUSIONS: LncRNA CEBPA-AS1 could alleviate cerebral ischemia-reperfusion injury by sponging miR-340-5p to regulate APPL1/LKB1/AMPK pathway.
Belching symptoms in gastroesophageal reflux disease, and prevalence and clinical characteristics of belching in Japanese adults
Authors: FUJIWARA YASUHIRO , Okuyama Masatsugu , Nagami Yasuaki , Taira Koichi , Ishizu Hirotaka , Takaishi Osamu , Sato Hiroshi , Watanabe Toshio 
Published: June 2020

Abstract
Background

Belching is the act of expelling gas from the stomach or esophagus noisily through the oral cavity. Although it is a physiological phenomenon, belching may also be a symptom of upper gastrointestinal diseases such as gastroesophageal reflux disease (GERD). A detailed epidemiology of belching has not yet been reported.
Aim

The aim of this study was to examine the prevalence and clinical characteristics of belching in Japanese adults.
Methods

We analyzed 1,998 subjects using data from a previous study of the association between GERD, psychological stress, and sleep disturbances in Japanese adults. Belching was evaluated according to the score to question 11 (‘Do you burp a lot?’) on the frequency scale for the symptoms of GERD (FSSG): 0 (never), 1 (occasionally), 2 (sometimes), 3 (often), and 4 (always). We also collected the clinical parameters, endoscopic findings, and data according to the Athens Insomnia Scale (AIS), Rome III questionnaire, and Hospital Anxiety and Depression scale (HADS).
Results

Subjects with GERD, especially non-erosive reflux disease, complained of belching more frequently. When we defined belching as an FSSG Q11 score ≥ 2 (sometimes or more frequent), 347 (17.4%) Japanese adults had belching. Subjects with belching were predominantly men and had more common functional dyspepsia. In addition, scores of HADS and AIS in subjects with belching were significantly higher compared to subjects without belching. Male sex, presence of functional dyspepsia, anxiety/depression, and sleep disturbances were significantly associated with a higher odds ratio for belching.
Conclusion

The epidemiology of belching in Japanese adults was clarified.
A Thermo-Dynamical Theory of Consciousness
Authors: bajaj gaurav 
Published: June 2020

Abstract
Humans have always thought of consciousness as property possessed by a living organism, without being able to properly define it in scientific terms. Many articles have been published to explain what consciousness is. In this article, consciousness has been defined as a quantity (‘variable’ would be a better word according to mathematics), which is linked with entropy of the system. The article explains consciousness, its role in the universe, not only in living systems, but also in non-living thermo-dynamical systems. Not only that, the article also explains its role with quantum biology, a newly emerging field of biology.
The Impact of Studying a Postgraduate Course in Clinical Biomechanics on Professional Practice
Authors: Branthwaite Helen , Calvert Sian , Chockalingam Nachiappan 
Published: June 2020

Abstract
Background There is a continual need for allied health professionals to maintain an active CPD portfolio for registration. Profession specific postgraduate studies have been shown previously to clinically enhance practice leading to extended scope and leadership roles. It is not well known though if completing an interprofessional, subject specific award has any impact on clinical practice. The aim of this paper was to retrospectively review what the clinical and professional practice impact was from gaining a postgraduate award in Clinical Biomechanics had on alumni’s practice. Methods 25 multidisciplinary alumni from Staffordshire University, Clinical Biomechanics suite of postgraduate awards, responded to a questionnaire designed to review any changes in clinical practice and the impact studying had had for participants. Results Thematic analysis of results highlighted trends in competency development and clinical impact of postgraduate study, indicating professional development and improved confidence in work interprofessional practices. Conclusion This study supports interprofessional cohorts of postgraduate study for a wider subject area, which has shown to enhance the evaluation of specific clinical practice whilst taking on a broader perspective of the topic, developing stronger and wider clinical conclusions.
Increased IGFBP7 Expression Correlates with Poor Prognosis and Immune Infiltration in Gastric Cancer: An Integrated Bioinformatics Analysis
Authors: Zhao Qiaoyun , Zhao Rulin , Song Conghua , Wang Huan , Rong Jianfang , Wang Fangfei , Yan Lili , Song Yanping , Xie Yong 
Published: June 2020

Abstract
Background Insulin-like growth factor binding protein-7 (IGFBP7) contributes to multiple biological processes in various tumors. However, the role of IGFBP7 in gastric cancer (GC) is still undetermined. The study aims to explore the role of IGFBP7 in GC via an integrated bioinformatics analysis.Methods IGFBP7 expression levels in GC and its normal gastric tissues were analyzed using multiple databases, including the Tumor Immune Estimation Resource (TIMER), Oncomine, The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. The methylation analysis was conducted with MEXPRESS, UALCAN and Xena online tools. The survival analysis was conducted using the Kaplan-Meier Plotter and Gene Expression Profiling Interactive Analysis (GEPIA) databases. Coexpressed genes of IGFBP7 were selected with the cBioPortal tool and enrichment analysis was conducted with the clusterProfiler package in R software. Gene set enrichment analysis (GSEA) was performed to explore the IGFBP7-related biological processes involved in GC. Correlations between IGFBP7 and immune cell infiltrates were analyzed using the TIMER database.Results IGFBP7 expression was significantly upregulated in GC and correlated with stage, grade, tumor status and Helicobacter pylori infection. High IGFBP7 expression and low IGFBP7 methylation levels were significantly associated with short survival of patients with GC. Univariate and multivariate analyses revealed that IGFBP7 was an independent risk factor for GC. The coexpressed genes LHFPL6, SEPTIN4, HSPB2, LAYN and GGT5 predicted unfavorable outcomes of GC. Enrichment analysis showed that the coexpressed genes were involved in extracellular matrix (ECM)-related processes. GSEA indicated that IGFBP7 was positively related to ECM and inflammation-related pathways. TIMER analysis indicated that the IGFBP7 expression level was strongly correlated with genes related to various infiltrating immune cells in GC, especially with gene markers of tumor associated macrophages (TAMs).Conclusions We demonstrate that increased IGFBP7 expression correlates with poor prognosis and immune cell infiltration in GC. IGFBP7 might be a potential biomarker for the diagnosis and targeted therapy for GC.
Occurrence of multi-drug resistant Escherichia coli and Escherichia coli O157:H7 in meat and swab samples of various contact surfaces at abattoir and butcher shops in Jimma town, Southwest district of Ethiopia
Authors: Abayneh Mengistu Sebsibe , Asfaw Eyob Tekalign 
Published: June 2020

Abstract
Background: Raw meat is one of commonly consumed traditional diets in Ethiopia. However, unhygienic processing and distribution practices were risky for contaminations of meat leads to human infection. This study was conducted to assess the presence of multi-drug resistant E. coli and E. coli O157:H7 in meats, ceacal content, and swabs samples of different contact surfaces at abattoir and butcher shops in Jimma town, Southwest Ethiopia. Methodology: A cross-sectional descriptive study was conducted from April to July, 2018. The isolation and identification processes passed through enrichment of samples with modified tryptone soy broth (mTSB), streaked onto MacConkey agar and Cefixime- tellurite sorbitol MacConkey agar, biochemical testing (indole and TSI), followed by latex agglutination test. Results: Out of 505 samples, 102(20.2%) and 27(5.4%) were positive for E. coli and E. coli O157:H7, respectively. Of these, 55(19.3%) and 47 (21.4%) of E. coli and 17 (6.0%) and 10 (4.5%) of E. coli O157:H7 were isolated from the abattoir and butcher shop samples, respectively. A significant difference in the occurrences was observed among sample sources. Antimicrobials susceptibility test result showed that, 92.2% to 96.1% of E. coli and 85.5% to 96.3% of E. coli O157:H7 were susceptible to third generation cephalosporin, ciprofloxacin, gentamycin, kanamycin, streptomycin and chloramphenicol. About 91.2% and 97.1% of E. coli and 88.9 % and 92.6% of E. coli 0157:H7 were resistant to ampicillin and erythromycin, respectively. A total of 98 (76.0%) E. coli and E. coli O157:H7 isolates were resistant to two or more classes of antibiotics. All abattoir and butcher shops workers were not have any formal education and training certificate on food safety and unhygienic practice were also observed.Conclusion: The presence of E. coli and E. coli O157:H7 including multi-drugs resistant isolates in raw meat highlights how the current meat processing and distribution practice was unhygienic. Therefore, strategies in the prevention and control of food borne infections that could be caused by multi-drug resistant strains will depends much on hygienic processing and distribution practices of meat.