bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of productive SafetyNet program on wasting among under-five children in the rural community of South Gondar Zone, Northwest Ethiopia
Authors: Engidaw Melaku Tadege , Gebremariam Alemayehu Digssie 
Published: October 2020

AbstractBackgroundUndernutrition is a significant public health problem in a developing country like Ethiopia. Even if the cause of malnutrition is multifactorial, it is mainly related to socioeconomical, political, and health-related problems. All these problems will lead to more severe nutrient deficiencies among households without assets. The Productive SafetyNet program is implemented for beneficiaries in the rural community to prevent household assets depletion. So, this study aimed to assess the effect of a Productive SafetyNet program on wasting among under-five children in the rural community of South Gondar Zone.MethodsA community-based cross-sectional study was done among 803 children paired with their mother/caregivers. Particepnts were selected by a simple random sampling technique. The data were collected by using a structured and pre-tested questionnaire. AnthroPlus software was used to analyse anthropometric data. The data entry employed by EpiInfo version 7.0 software for Windows. Then, the data exported to SPSS version 20.0 to carryout further statistical analysis. The anthropometric index (weight for height) was constructed to determine wasting. Both binary and multivariable logistic regression models were used to identify associated factors. Finally,P-Value ≤0.05 was used to declare statistical significance.ResultsIn this study, the response rate was 95.76%. Of the total participants, 195(25.36%) children were from SafetyNet program beneficiaries. The overall prevalence of wasting was 29.9% (95% CI: 26.6, 33.2%). More than One-fourth of the children from SafetyNet beneficiary households were wasted. While considering all other variables constant, Productive SafetyNet Program reduce wasting by 46% (COR = 0.54. 95% CI (0.37, 0.79)). Wasting were significantly associated with marital status (divorced and/or separated: AOR = 3.33, 95% CI (1.71, 6.45)), being on the SafetyNet program (AOR: 0.63, 95% CI (0.40, 0.99)), family size (AOR = 0.13, 95% CI (0.09, 0.21)), father educational status (AOR: 0.25, 95% CI (0.09, 0.66)), age of the child (AOR = 0.51, 95% CI (0.33, 0.77)), and child dietary diversity score (AOR = 2.99, 95% CI (1.67, 5.35)).ConclusionWasting was a severe public health problem. In this study, the Productive SafetyNet Program reduce wasting significantly. Marital status, SafetyNet program status, family size, father educational status, age of the child, and dietary diversity were factors associated with wasting among children. Early detection of household asset depletion and SafetyNet program implementation is vital with the usual nutritional assessment and counseling.
Ecological and floristic study of Hirmi woodland vegetation in Tigray Region, Northern Ethiopia
Authors: Girmay Mehari , Bekele Tamrat , Demissew Sebsebe , Lulekal Ermias 
Published: October 2020

AbstractThe dryland area in Ethiopia covers a substantial region endowed with diverse plant resources. However, the landmass has received less attention even if it has high ecological, environmental, and economic uses. The present study was conducted in Hirmi woodland vegetation, which is one of the dryland areas in Ethiopia, with the objective of investigating the floristic composition, plant community types, vegetation structure, community-environment relations and its regeneration status. Vegetation and environmental data were collected from 80 sampling plots with a size of 25 m × 25 m designated as the main plots. Diameter at breast height (DBH), height, basal area, density, vertical structure, importance value index (IVI), and frequency were computed. Species diversity and evenness were analyzed using Shannon diversity and evenness indices. The plant community types and vegetation-environment relationships were analyzed using classification and ordination tools, respectively. A total of 171 vascular plant species belonging to 135 genera and 56 families were recorded. About 5.3% of the species were endemic and near-endemic to Ethiopia. The highest number of species was recorded in families Fabaceae (16.4%) and Poaceae (11.7%) followed by Asteraceae (7.0%), Combretaceae, Lamiaceae, and Moraceae (3.5% each). Five plant communities were identified. According to the results from ordination analysis, the floristic composition of these plant communities was significantly affected by altitude, slope, sand, silt, soil organic matter, total nitrogen, and disturbance. The vegetation structure reveals that a large number of individual species was categorized in the lower DBH, frequency, and height classes. The highest Shannon diversity index and evenness values of the study area were 4.21 and 0.95, respectively.Anogeissus leiocarpa, Combretum hartmannianum, Ziziphus mucronata, Terminalia macroptera,andAcacia polyacanthawere the species with high IVI. Some endemic plants were in the IUCN red list categories of Ethiopia and Eritrea. The overall regeneration status of the study area was poor because of anthropogenic disturbances and grazing pressures. Although the study area is endowed with high plant species diversity including endemism, it is under poor regeneration status due to various disturbances. To overcome this challenge, integrated management measures including monitoring and application of restoration techniques by taking into consideration the significant environmental factors associated with species diversity as well as observed regeneration status and IUCN threat level of the species are highly recommended.
Effects of geoclimatic factors on the variability in Pinus pinea cone, seed, and seedling traits in Turkey native habitats
Authors: Balekoglu Safa , Caliskan Servet , Dirik Hüseyin 
Published: October 2020

Abstract
Background
Stone pine (Pinus pinea L.) is harvested for its edible kernels. It is an important forest tree species, and also plays an important role in afforestation. Successful afforestation activities strongly depend on the quality of seeds and seedlings. Five eastern native populations of stone pine were analyzed, in terms of their geographical and climatic parameters, in order to identify their cone, seed, pine nut, and seedling traits. With this regard, we aimed to contribute to the reforestation, yield, and breeding of this species.

Results
All native populations occur in the sub-humid bioclimatic zone, according to Emberger’s bioclimatic classification, ranging 74.7–63.2 of Emberger’s Q Index value (Q), which synthesizes humidity and temperature, with lower values indicating more arid conditions. With regard to geographical factors, altitude was found to have the greatest effect on cone diameter and seed weight, while there was a weak correlation of cone, seed, and pine nut dimension with longitude and latitude. In terms of climatic factors, Q and mean of the maximum temperature of June, July, and August (ME) were found to be the main drivers behind significant effects in cone, seed, and pine nut morphometric traits. The most negative effect on seed number cone−1 (r: − 0.897), pine nut number cone−1 (r: − 0.923), pine nut yield (r: − 0.903), and pine nut weight (r: − 0.878) was Q, whereas cone diameter, seed length, and seed weight were positively correlated with the ME (r: 0.939, r: 0.889, and r: 0.866, respectively). Cone and seed diameter and seed weight increased with decreasing summer drought index (r: − 0.806, r: − 0.846, and r: − 0.866, respectively). Rainfall in the June of the third year prior to harvest time (RJ) had a negative effect on the percentage of sound seeds per cone. Annual rainfall in the third year prior to harvest time (AR) significantly affected pine nut weight (r: 0.889), although rainfall during summer had a negative effect. The empty seed ratio of the different populations ranged from 20 to 68%, while considering all populations, the empty seed ratio was 46%.

Conclusions
The study suggests that the Q Index and ME should be taken into account to determine cone to pine nut yield. With this regard, plantations of stone pine should be established on sub-humid areas and Q values should not exceed 75.
Prevalence and distribution of resistance and enterotoxins/enterotoxin-like genes in different clinical isolates of coagulase-negative Staphylococcus
Authors: Nasaj Mona , Saeidi Zahra , Tahmasebi Hamed , Dehbashi Sanaz , Arabestani Mohammad Reza 
Published: October 2020

Abstract
Background
Coagulase-negative staphylococcus (CoNS) is considered to be the major reservoirs for genes facilitating the evolution of S. aureus as a successful pathogen. The present study aimed to determine the occurrence of genes conferring resistance to fluoroquinolone, determining of the prevalence of insertion sequence elements IS256, IS257 and different superantigens (SAgs) among CoNS isolates obtained from various clinical sources.

Materials and methods
The current study conducted on a total of the 91 CoNS species recovered from clinical specimens in Hamadan hospitals in western Iran in 2017–2019. The antimicrobial susceptibility testing was performed using disk diffusion method and the presence of the IS256 and IS257, genes conferring resistance to fluoroquinolone and enterotoxins/enterotoxin-like encoding genes were investigated by polymerase chain reaction (PCR) method.

Results
Among genes encoding classic enterotoxins, sec was the most frequent which was carried by 48.4% of the 91 isolates, followed by seb in 27.5% of the isolates. None of the CoNS isolates was found to be positive to enterotoxin-like encoding genes. In 11(12%) of all isolates that were phenotypically resistant to levofloxacin, 9 isolates (81.8%) were positive for gyrB, 8 isolates (72.7%) were positive for gyrA, 8 isolates (72.7%) harbored grlB and 7 isolates (63.6%) were found to carry grlA. The IS256 and IS257 were identified in 31.8% and 74.7% of the isolates, respectively. The results of statistical analysis showed a significant association between the occurrence of staphylococcal enterotoxins (SEs) encoding genes and antimicrobial resistance.

Conclusion
Antimicrobial resistant determinants and SEs are co-present in clinical CoNS isolates that confer selective advantage for colonization and survival in hospital settings. The coexistence of insertion elements and antibiotic resistance indicate their role in pathogenesis and infectious diseases.
Dietary L-citrulline supplementation modulates nitric oxide synthesis and anti-oxidant status of laying hens during summer season
Authors: Uyanga Victoria A. , Jiao Hongchao , Zhao Jingpeng , Wang Xiaojuan , Lin Hai 
Published: October 2020

AbstractBackgroundL-citrulline (L-Cit), a non-protein amino acid, has been implicated in several physiological functions including anti-inflammatory, anti-oxidative, and hypothermic roles, however, there is a paucity of information with regards to its potential in poultry production.MethodsThis study was designed to investigate the effects of dietary L-Cit supplementation on the production performance, nitric oxide production, and antioxidant status of laying hens during summer period. Hy-Line Brown laying hens (n = 288, 34 weeks old) were allotted to four treatment, 6 replicates of 12 chickens each. Dietary treatments of control (basal diets), 0.25%, 0.50% and 1.00% L-Cit supplementation were fed to chickens for eight (8) weeks. Production performance, free amino acid profiles, nitric oxide production, and antioxidant properties were measured. Blood samples were collected at the 4thand 8thweeks of the experiment.ResultsAir temperature monitoring indicated an average daily minimum and maximum temperatures of 25.02 °C and 31.01 °C respectively. Dietary supplementation with L-Cit did not influence (P > 0.05) the production performance, and rectal temperature of laying hens. Egg shape index was increased (P < 0.05) with increasing levels of L-Cit. Serum-free content of arginine, citrulline, ornithine, tryptophan, histidine, GABA, and cystathionine were elevated, but taurine declined with L-Cit diets. Plasma nitric oxide (NOx) concentration was highest at 1% L-Cit. Likewise, nitric oxide synthase (NOS) activity for total NOS (tNOS) and inducible NOS (iNOS) were upregulated with increasing L-Cit levels, although, tNOS was not affected at the 4thweek. Anti-oxidant enzymes including catalase and superoxide dismutase (SOD) were increased with L-Cit supplementation, however, SOD activity was unchanged at 4thweek, while total anti-oxidant capacity increased at the 8thweek. L-Cit supplementation attenuated the extent of lipid peroxidation, and also inhibited glutathione peroxidase activity.ConclusionDietary L-Cit supplementation modulated systemic arginine metabolism, nitric oxide synthesis, antioxidant defense system, and increased the egg shape index of laying hens during the summer season. 1% L-Cit supplementation proved most effective in potentiating these effects and may be adopted for feed formulation strategies.
Public spending on orphan medicines: a review of the literature
Authors: Gombocz Margit , Vogler Sabine 
Published: October 2020

Abstract
Background and objective
Little is known about how much public payers spend on orphan medicines. This study aimed at identifying information on orphan medicine expenditure incurred by public payers that was published in literature globally and at possibly synthesising their shares as portion of the total pharmaceutical expenditure.

Methods
A literature review was undertaken using Medline, the Orphanet Journal of Rare Diseases and Google Scholar. Titles and abstracts were screened, and full texts of potentially qualifying studies were reviewed for inclusion. Included articles were analysed, and bibliometric parameters as well as public expenditure data on orphan medicines were retrieved.

Results
Six hundred forty three articles excluding duplicates were identified. After screening of the abstracts and a review of the full texts, 13 articles qualified for in-depth analysis.
The 13 selected articles on orphan pharmaceutical expenditure were published between 2010 and 2018. Survey periods varied between 1 year and 12 years. One publication included 22 countries but the majority of the studies were related to a single country. Expenditure data was available in five of the 13 articles, and eight articles used ‘expenditure proxies’ such as sales data. Spending data had been sourced from public institutions (4 studies), private providers (5 studies) and a combination of both (3 studies, no information on data source in 1 study). In all included studies, secondary data were analysed. Reported expenditure shares for orphan medicines in relation to total pharmaceutical spend was frequently below 3%. Countries with higher shares included the USA, Canada, the Netherlands and Bulgaria—the latter reporting spending on orphan medicines as high as 9%.

Conclusions
A low number of studies that informed about pharmaceutical spending on orphan medicines was published, thereof only a few explicitly analysed expenditure data of public payers. A conclusive synthesis of public spending on orphan medicines is a challenge given to the diversity in methodologies to measure expenditure. There is a need for further research to survey primary data of public spending for orphan medicines, based on a sound methodology to measure these data and to compare them internationally.
Inquiry-based learning and E-learning: how to serve high and low achievers
Authors: Sotiriou Sofoklis A. , Lazoudis Angelos , Bogner Franz X. 
Published: October 2020

AbstractLarge-scale implementations of effective inquiry-based learning are rare. A European-wide initiative gave teachers access to innovative e-learning tools (ranging from virtual labs, virtual games and simulations to augmented reality applications) for lesson planning and classroom implementation. We examined 668 such implementations across 453 schools within the period of one school year. Teachers could use a platform with digital resources and tools and were encouraged to adopt five different phases of inquiry-based learning: orientation, hypothesizing, planning, analysis, and conclusion. Additionally, an integrated interface for lesson implementation tracked each students’ problem-solving competence (during the inquiry lessons), culminating in about 12,000 datasets. Every user generated an average of 22 digital inquiry-based digital scenarios, each of which required approximately 50.14 min for completion. These scenarios, using high quality resources adapted to school conditions, yielded significant learning outcomes for participating students (age: 14.4 years, gender balanced). While the PISA study identified 10% high achievers on average, we exceeded this number in our framework scoring 20–29% high achievers and 37–42% low achievers (which was close to the 45% PISA average). Offering tools to teachers, which help creating individual inquiry scenarios and monitoring students’ achievement, does not yield any insurmountable obstacles for classroom-implementation of inquiry-based lessons: Compared to the PISA study, levels of high achievers increased even if complex problem-solving competence was required.
Discoid lateral meniscus: importance, diagnosis, and treatment
Authors: Kim Jun-Ho , Ahn Jin Hwan , Kim Joo-Hwan , Wang Joon Ho 
Published: October 2020

AbstractDiscoid lateral meniscus (DLM) is a common anatomic variant in the knee typically presented in young populations, with a greater incidence in the Asian population than in other populations. As DLM is a congenital anomaly, the ultrastructural features and morphology differ from those of the normal meniscus, potentially leading to meniscal tears. Snapping and pain are common symptoms, with occasional limitations of extension, in patients with DLM. Examination of the contralateral knee is necessary as DLM affects both knees. While simple radiographs may provide indirect signs of a DLM, magnetic resonance imaging (MRI) is essential for diagnosis and treatment planning. Although DLM was traditionally classified into three categories, namely, complete, incomplete, and Wrisberg DLM, a recent MRI classification provides useful information for surgical planning because the MRI classification was based on the peripheral detachment in patients with DLM, as follows: no shift, anterocentral shift, posterocentral shift, and central shift. Asymptomatic patients require close follow-up without surgical treatment, while patients with symptoms often require surgery. Total or subtotal meniscectomy, which has been traditionally performed, leads to an increased risk of degenerative arthritis; thus, partial meniscectomy is currently considered the treatment of choice for DLM. In addition to partial meniscectomy, meniscal repair of peripheral detachment is recommended for stabilization in patients with DLM to preserve the function of the meniscus. Previous studies have reported that partial meniscectomy with or without meniscal repair is effective and shows superior clinical and radiological outcomes to those of total or subtotal meniscectomy during the short- to long-term follow-up. Our preferred principle for DLM treatment is reduction, followed by reshaping with reference to the midbody of the medial meniscus and repair as firm as possible.
Selection markers for transformation of the sequenced reference monokaryon Okayama 7/#130 and homokaryon AmutBmut of Coprinopsis cinerea
Authors: Dörnte Bastian , Peng Can , Fang Zemin , Kamran Aysha , Yulvizar Cut , Kües Ursula 
Published: October 2020

Abstract
Background
Two reference strains have been sequenced from the mushroom Coprinopsis cinerea, monokaryon Okayama 7/#130 (OK130) and the self-compatible homokaryon AmutBmut. An adenine-auxotrophy in OK130 (ade8-1) and a para-aminobenzoic acid (PABA)-auxotrophy in AmutBmut (pab1-1) offer selection markers for transformations. Of these two strains, homokaryon AmutBmut had been transformed before to PABA-prototrophy and with the bacterial hygromycin resistance marker hph, respectively.

Results
Gene ade8 encodes a bifunctional enzyme with an N-terminal glycinamide ribonucleotide synthase (GARS) and a C-terminal aminoimidazole ribonucleotide synthase (AIRS) domain required for steps 2 and 5 in the de novo biosynthesis of purines, respectively. In OK130, a missense mutation in ade8-1 rendered residue N231 for ribose recognition by the A loop of the GARS domain into D231. The new ade8+ vector pCcAde8 complements the auxotrophy of OK130 in transformations. Transformation rates with pCcAde8 in single-vector and co-transformations with ade8+-selection were similarly high, unlike for trp1+ plasmids which exhibit suicidal feedback-effects in single-vector transformations with complementation of tryptophan synthase defects. As various other plasmids, unselected pCcAde8 helped in co-transformations of trp1 strains with a trp1+-selection vector to overcome suicidal effects by transferred trp1+. Co-transformation rates of pCcAde8 in OK130 under adenine selection with nuclear integration of unselected DNA were as high as 80% of clones. Co-transformation rates of expressed genes reached 26–42% for various laccase genes and up to 67% with lcc9 silencing vectors. The bacterial gene hph can also be used as another, albeit less efficient, selection marker for OK130 transformants, but with similarly high co-transformation rates. We further show that the pab1-1 defect in AmutBmut is due to a missense mutation which changed the conserved PIKGT motif for chorismate binding in the C-terminal PabB domain to PIEGT in the mutated 4-amino-4-deoxychorismate synthase.

Conclusions
ade8-1 and pab1-1 auxotrophic defects in C. cinerea reference strains OK130 and AmutBmut for complementation in transformation are described. pCcAde8 is a new transformation vector useful for selection in single and co-transformations of the sequenced monokaryon OK130 which was transformed for the first time. The bacterial gene hph can also be used as an additional selection marker in OK130, making in combination with ade8+ successive rounds of transformation possible.
Immunopathogenesis of SARS-CoV-2-induced pneumonia: lessons from influenza virus infection
Authors: Miyazawa Masaaki 
Published: October 2020

AbstractFactors determining the progression of frequently mild or asymptomatic severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection into life-threatening pneumonia remain poorly understood. Viral and host factors involved in the development of diffuse alveolar damage have been extensively studied in influenza virus infection. Influenza is a self-limited upper respiratory tract infection that causes acute and severe systemic symptoms and its spread to the lungs is limited by CD4+ T-cell responses. A vicious cycle of CCL2- and CXCL2-mediated inflammatory monocyte and neutrophil infiltration and activation and resultant massive production of effector molecules including tumor necrosis factor (TNF)-α, nitric oxide, and TNF-related apoptosis-inducing ligand are involved in the pathogenesis of progressive tissue injury. SARS-CoV-2 directly infects alveolar epithelial cells and macrophages and induces foci of pulmonary lesions even in asymptomatic individuals. Mechanisms of tissue injury in SARS-CoV-2-induced pneumonia share some aspects with influenza virus infection, but IL-1β seems to play more important roles along with CCL2 and impaired type I interferon signaling might be associated with delayed virus clearance and disease severity. Further, data indicate that preexisting memory CD8+ T cells may play important roles in limiting viral spread in the lungs and prevent progression from mild to severe or critical pneumonia. However, it is also possible that T-cell responses are involved in alveolar interstitial inflammation and perhaps endothelial cell injury, the latter of which is characteristic of SARS-CoV-2-induced pathology.