bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Two Novel Therapeutics Against Human and Canine Osteosarcoma
Authors: Wilson-Robles Heather , Miller Tasha , Sima Chao , Hua Jianping , Cypert Milana , Bittner Michael 
Published: August 2020

Abstract
Background: Osteosarcoma (OS) is the most common primary bone tumor in both humans and canines. This tumor has an aggressive course leading to the development of metastatic lesions in most patients diagnosed with this disease. Two new novel agents, MLN9708 and SH4-54, work as a proteasome inhibitor and a STAT3 inhibitor, respectively. Targets of these drugs have been shown to be overexpressed in OS in both species. Methods: Two human and two canine OS cell lines were exposed in vitro to both drugs alone and in combination. The number of cells undergoing apoptosis, as well as the number of cells capable of invasion through a matrigel basement membrane was evaluated after exposure to the drugs. Additionally, PCR and Western blots of downstream targets were evaluated. Finally, both drugs were tested against each cell line in an in vivo murine xenograft model. Results: All four cell lines were sensitive to MLN9708, one of the human cell lines and both canine cell lines were resistant to SH4-54. MLN9708 was also better at inhibiting invasion in three of the four cell lines. In the murine xenografts MLN9708 inhibited growth and metastasis in 143B (human OS) and the combination inhibited growth in the canine OS cell line (MCKOS). Conclusions: Though SH4-54 demonstrated robust cell killing in 143B in vitro, MLN9708 demonstrated broader activity across species for the treatment of OS. Further investigation into this drug is warranted as a treatment for OS. Combination of this drug with a STAT3 inhibitor may be worthwhile in canine OS.
Effects of CT images with different reconstruction energy on radiotherapy planning for patients with non-small cell lung cancer
Authors: Xiufang Tian , Kun Liu , Jing Wang , Jiandong Zhang , Hou Yong 
Published: August 2020

Abstract
BACKGROUND

With the development of the CT technology, multi-energy technology is applied to CT imaging, which improves the time resolution, spatial resolution and density resolution of CT system. It enables the CT imaging system to achieve clearer image display under safe and low-dose conditions, so that the disease can be displayed more quickly, and the disease can be identified more early and more clearly.
METHODS

38 non-small cell lung cancer patients were selected for energy spectral scanning. All energy spectral images obtained were transferred to the DiscoverTM CT post-processing workstation to generate 40 keV, 60 keV, 80 keV, 100 keV, 120 keV, and 140 keV single-energy images. Then the single-energy images were imported to Eclipse, and the oncologist contours the target area and organs at risk (OARs) on the single-energy images described above, and then the physicist designed radiotherapy plans to perform statistical analysis on the tissue CT value and target volume of each single-energy image, and to compare dosimetry of different plans about the organs at risk and the target area.
RESULTS

The CT values of GTV, heart, lung, and spinal cord of different energy CT images are statistically different (P < 0.05). Among them, the CT value of each tissue obtained in the 40 keV group is the largest, and the CT value decreases with the increase of energy. There were no statistically significant differences in the homogeneity index(HI), the conformity index(CI), the maximum dose, the minimum dose and the average dose of the gross tumor volume (GTV) delineated on CT images of different energy (P > 0.05), as well as the organs at risk.
CONCLUSIONS

When CT images of different energies obtained from energy spectral CT scans are used in the design of radiotherapy planning, there are no significant differences in target area outlines and doses caused by energy factors, but the differences in tissue CT values have statistical significant.
Relationship between BMI with percentage body fat and obesity in Singaporean adults – The Yishun Study.
Authors: Chen Kexun Kenneth , Wee Shiou-Liang , Pang Benedict , Lau Lay Khoon , Jabbar Khalid Abdul , Seah Wei Ting , Ng Tze Pin 
Published: August 2020

Abstract
Background: Primary aim of this study is to the determine relationship between BMI and BF% in Singaporean adults, derive a prediction model to estimate BF%, and to report population BF%. Secondary aim is to determine the prevalence of overweight and obesity based on BF% threshold and the new risk categories for obesity in Singaporean population.Methods: This is a population-based study of 542 community-dwelling Singaporeans (21-90 years old, 43.1% men). Anthropometry, body composition, and questionnaire-based lifestyle factors were assessed. Relationship between BMI and BF% were analysed using multiple regression model. Prevalence of overweight and obesity were estimated using WHO and Singapore Ministry of Health (MOH) Clinical Practice Guidelines BMI classification, BF% of 25% and 35% for men and women . Results: We derived a prediction model to estimate BF% based on BMI, age, sex and ethnicity. The current cohort of Singaporeans have higher BF% at matching BMI, age and sex than Caucasians, and a Singaporean cohort from 20 years ago. The overall population-adjusted prevalence of obesity according to WHO International classification (BMI ³30kg/m2) was 12.9% (14.9% men; 11.0% women); and 26.6% (30.7% men; 22.8% women) according to MOH classification (BMI ³27.5kg/m2). However, using BF% cut-off (>25% for men and >35% for women), prevalence of obesity increased to 82.0% (80.2% men; 83.8% women).Conclusion: There appears to be a large discrepancy between BF% and BMI measured obesity in Singaporean adults. The results confirmed that Singaporean adults have higher BF% at lower BMI compared to Caucasians; and that BF% in our population have increased over two decades.
Proteomics Analysis and Identification of Critical Proteins and Network Interactions That Regulate the Specific Deposition of IMF of Jingyuan Chicken
Authors: Huang Zengwen , Zhang Juan , Gu Yaling , Xin Guosheng , Cai Zhengyun , Yin Xianfeng , Feng Xiaofang , Mu Tong , Yang Chaoyun 
Published: August 2020

Abstract
Background:Improving the production efficiency and quality of broiler chickens has become a hot topic in molecular breeding of the broiler industry. There are several factors, among which intramuscular fat (IMF) content is one of the essential factors determining chicken quality. The chicken carcass weight of 40-50% consisted of the leg muscles and breast muscles, and the research on chicken meat quality was performed on that part. Jingyuan chicken is one of the high-quality local chicken species in China. However, the molecular mechanism of IMF specific deposition in different parts of Jingyuan chicken is still unclear. In order to screen and identify the proteins that regulate IMF specific deposition in different parts of Jingyuan chicken and analyze network interaction, the present study selected 180-day-old Jingyuan chicken as the research material, using proteomic techniques and PRM experimental methods to carry out basic research on leg muscle and breast muscle. Results:Three key signaling pathways and ten essential functional proteins regulating IMF specific deposition of Jingyuan chicken were identified, while the molecular mechanism of the deposition of proteins was explained, and analyzed the network interaction between these key functional proteins. Conclusions:The results of this study are of great scientific value and theoretical significance not only for improving chicken quality but also for molecular breeding of high-quality local chickens.
Geographical variation and factor associated with modern contraceptive utilization among Young married women aged between 15-24 years: Spatial and multilevel analysis of EDHS 2016.
Authors: Yesuf Kedir Abdu , Muluneh Aysheshum , Asrese Eueail Teferi 
Published: August 2020

Abstract
Introduction: Despite increase in trend of contraceptive utilization in worldwide it was still low in developing country. In Ethiopia modern contraceptive utilization among young married women was low which shows gaps between women reproductive desire to avoid pregnancy and contraceptive behavior. This study shows varation in modern contraceptive utilization and factor associated with it among young merried age group between 15-24 years in Ethiopia. Objective: The aim of the study was to assess the geographical varation of modern contraceptive utilization and associated factor among young married women aged between 15- 24 year in Ethiopia. Methods: Cross-sectional study design was applied using Ethiopia demographic and health survey 2016 data. The sample size was 2298 young married women aged between 15-24 years. Spatial analysis was done using spatial autocorrelation Moran’s I, Gettis-OrdGi* and spatial scan statics to identify significant clusters of modern contraceptive utilization. The data were analyzed using a two level-mixed-effects logistic regression model to determine the individual and community level factors associated with modern contraceptive utilization.Result: In Ethiopia, prevalence of modern contraceptive utilization among young married women age group 15-24 years was 36.7 %. Spatial scan statistics identified primary clusters of modern contraceptive utilization were all zone of Amhara, Addis Ababa and Shewa. In other hand, Gettis-OrdGi* analysis indicated zone 1 of Afar region, Borena zone of Oromia region and all zone of Somalia regions were low prevalence spot region. In this study religion, wealth index, religious, health facility visits within 12 months, husband desire more children, perception of distance from health facility, region, Community access to health services and community educational level were statically significant variables for modern contraceptive utilization.Conclusions: There is low prevalence of modern contraceptive utilization and it was varied across zone of Ethiopia. High and low prevalence spot area, most likely cluster, community and individual level factor associated with modern contraceptive utilization is identified which is important to prioritize family planning strategy. Therefore, exerting much effort on this area is supreme important as it has significant public health contributions.
Association of β1 and β2-adrenoceptor Polymorphisms With the Demand of Inotropic Catecholamine Support Following Coronary Artery Bypass Grafting in Iranian Population
Authors: Eslami Gohar , Ghafari Rahman , Habibi Valiollah , Hedayatizadeh-Omran Akbar , Moosazadeh Mahmood , Amjadi Omolbanin , Alikhani Reza , Emami Shahaboddin 
Published: August 2020

Abstract
Background: Hemodynamic instability is a common complication in the first hours following cardiac surgery and inotropic catecholamine support is an acceptable treatment strategy for its management. β1 and β2-adrenoceptors (β1 and β2 AR) are mediated the positive inotropic and chronotropic responses of the heart to catecholamines. Previous evidence has suggested an association between β1 and β2AR polymorphisms and cardiac response and change in receptor signaling. The aim of this study was evaluating the relationship between β1 and β2AR polymorphisms with demand of catecholamine inotropic support among coronary artery bypass grafting (CABG) patients.Methods: One hundred ninety-eight consecutive patients who underwent CABG with cardiopulmonary bypass were included in this study. We assessed hemodynamic parameters, dose and duration of inotropic support according to β1 and β2AR genotypes in post-operative period. DNA genotyping were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR genotyping results were confirmed by direct DNA sequencing. Results: The our results indicated that patients carrying one or two alleles of the Arg389-β1AR variant required significantly shorter inotropic support time compared with patients homozygous for the Gly389-β1AR (p=0.003). Finally, neither β1AR polymorphisms nor Arg16Gly-β2AR polymorphism are associated with catecholamines-induced hemodynamic effects.Conclusion: These findings suggest that genetic variability in the β1 and β2AR polymorphisms may not be a major determinant of cardiac responses to catecholamine treatment in Iranian population. However, larger scale studies with different ethnicities are needed for confirmation.
Production of Proline and Protease With Different Organic Wastes in Bacteria
Authors: Kahraman Hüseyin , Karaderi Cennet Canan 
Published: August 2020

Abstract
Background: In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. Methods: 5 ml of different organic waste materials were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Results: Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using TCW, WCW and OWW organic waste. Conclusion: We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment.
Promoting health behaviors for preventing and controlling noncommunicable disease among North Korean defectors: A systematic review
Authors: Shin Hyun-Young , Shin Jung Eun , Baek Seung Yon , Chu Sang Hui 
Published: August 2020

Abstract
Background: Recently, noncommunicable disease (NCDs) has emerged as a new major health issue in North Korea. However, studies on the prevalence of its risk factors and their related health behavior among North Koreans are lacking. The studies on North Korean defectors could help us in understating their awareness and health‑promoting behavior as well as their vulnerabilities for metabolic diseases or NCDs under the current circumstances where South and North Korea have limited exchanges in the health and medical sectors. In this study, we reviewed the prevalence of risk factors or the health‑promoting behavior related to NCD among North Korean defectors. Methods: Search on the PubMed (MEDLINE), CINAHL, Cochrane Library, EMBASE, Korean Nursing Database, Korean Medical Database, KIHASA Repository, and RISS were performed. We included all study types reporting any type of risk factors or prevalence or health behaviors with the following inclusion criteria: (1) published in English or Korean; (2) NCD‑related topic; and (3) age ≥18 years. The data were extracted independently by the 2 authorsResults: Finally, 22 studies were included in the analysis: 18 quantitative study and 4 qualitative studies, and among the quantitative studies, 5 studies had comparisons with South Koreans. The number of participants ranged from 8–932 participants, and 54.6%–100% of them were women. The survey using the questionnaires was the most popular research method. Data from objective measurements, such as body weight, waist circumference, blood test, and urine analysis were used in a few studies. North Korean defectors were found to have higher smoking rates, drinking rates, and increased abdominal obesity caused by the lack of awareness and management of risk factors since they moved to South Korea.Conclusions: In this study, we showed that there were not enough studies related to NCDs and health behaviors among North Korean defectors. Nevertheless, North Korean defectors were found to be more vulnerable to NCDs, including metabolic syndrome. It is necessary to improve the awareness of NCD and its risk factors among North Korean defectors. Efforts for promoting North Korean defectors’ health behaviors by the government and related professional organizations are needed.
Heterogeneous Academic Network Embedding Based Multivariate Random-Walk Model for Predicting Scientific Impact
Authors: Qiao Yongwei , Sun Leilei , Han Jianing , Xiao Chunjing 
Published: August 2020

Abstract
The prediction of current scientific impact of papers and authors has been extensively studied to help researchers find valuable papers and recent research directions, also help policymakers make recruitment decisions or funding allocation. However, how to accurately evaluate the future impact of them, especially for new papers and young researchers, is the focus of scientific impact prediction research, and is less explored. Existing graph-based methods heavily depend on the global structure information of heterogeneous academic network and ignore the local structure information and text information, which may provide important clues to identify influential papers and authors with novel perspective. In this paper, we propose a hybrid model called ESMR to predict the future influence of papers and authors by mainly exploiting these information mentioned above. Specifically, we first put forward a novel network embedding-based model, which can capture not only the local structure information, but also the text information of papers into a unified embedding representation. Then, the future impact of papers and authors is mutually ranked by integrating the learned embedding representations into a multivariate random-walk model. Empirical results on two real datasets demonstrate that the proposed method significantly outperforms the existing state-of-the-art ranking methods.
Dysregulation of macrophage PEPD in obesity determines adipose tissue fibro-inflammation and insulin resistance
Authors: Pellegrinelli Vanessa , Rodriguez-Cuenca Segio , Rouault Christine , Schilbert Hanna , Virtue Sam , Moreno-Navarrete Jose Maria , Bidault Guillaume , Borrego Maria del Carmen Vázquez , Dias Ana Rita , Pucker Boas , Dale Martin , Campbell Mark , Carobbio Stefania , Aron-Wisnewsky Judith , Mora Silvia , Masiero Mauro , Emmanouilidou Anastasia , Mukhopadhyay Subhankar , Dougan Gordon , Hoed Marcel den , Loos Ruth , Fernández-Real José Manuel , Chiarugi Davide , Clément Karine , Vidal-Puig Antonio 
Published: August 2020

Abstract
Fibrosis is a hallmark of adipose tissue (AT) dysfunction and obesity-associated insulin resistance that results from an impaired collagen turnover. Peptidase D (PEPD) plays a vital role in collagen turnover by degrading proline-containing dipeptides. Nevertheless, its specific function and importance in AT is unknown. GWAS identified the rs731839 variant in the locus near PEPD that uncouples obesity from insulin resistance and dyslipidaemia, thus indicating that defective PEPD might impair AT remodelling and exacerbate metabolic complications. Here we show that in human and murine obesity, PEPD expression and activity decrease in AT, coupled to the release of PEPD systemically. Both events, in turn, are associated with the accumulation of fibrosis in AT and insulin resistance. Using pharmacologic and genetic animal models of PEPD down-regulation, we show that whereas dysfunctional PEPD activity provokes AT fibrosis, it is the PEPD secreted by AT the main contributor to inflammation, insulin resistance and metabolic dysfunction. Also, PEPD originated in inflammatory macrophages (Mɸ), plays an essential role promoting fibro-inflammatory responses via activation of EGFR in Mɸ and preadipocytes. Using genetic ablation of pepd in Mɸ that prevents obesity-induced PEPD release, also averts AT fibro-inflammation and obesity-associated metabolic dysfunctions. Taking advantage of factor analysis, we have identified the coupling of prolidase decreased activity and increased systemic levels of PEPD as the essential pathogenic triggers of AT fibrosis and insulin resistance. Thus, PEPD produced by Mɸ qualifies as a biomarker of AT fibro-inflammation and a therapeutic target for AT fibrosis and obesity-associated insulin resistance and type 2 diabetes.
Disrupted Mitochondrial and Metabolic Plasticity Underlie Comorbidity Between Age-related and Degenerative Disorders as Parkinson Disease and Type 2 Diabetes Mellitus
Authors: Juárez-Flores Diana Luz , Ezquerra Mario , Gonzàlez-Casacuberta Íngrid , Ormazabal Aida , Morén Constanza , Tolosa Eduardo , Fucho Raquel , Guitart-Mampel Mariona , Casado Mercedes , Valldeoriola Francesc , Torre-Lara Joan de la , Muñoz Esteban , Tobías Ester , Compta Yaroslau , García-García Francesc Josep , Garcia-Ruiz Carmen , Fernandez-Checa Jose Carlos , Marti Maria José , Grau Josep Maria , Cardellach Francesc , Artuch Rafael , Fernández-Santiago Rubén , Garrabou Glòria 
Published: August 2020

Abstract

Background. Idiopathic Parkinson’s disease (iPD) and Type 2 Diabetes Mellitus (T2DM) are chronic, degenerative, multisystemic and age-related diseases, with eventual epidemiological co-morbidity and potential overlap in molecular basis. This study aims to explore if metabolic and mitochondrial alterations underlie the suggested epidemiologic and clinical co-morbidity at molecular level. Methods. To evaluate the adaptation of iPD to a simulated pre-diabetogenic state, we exposed primary cultured fibroblasts from iPD patients and controls to standard (5mM) and high (25mM) glucose concentrations to further characterize metabolic and mitochondrial resilience.Results. iPD fibroblasts showed increased levels of organic and amino acid levels related to mitochondrial metabolism with respect to controls, and these differences were enhanced in high glucose conditions (citric, suberic and sebacic acids levels increased, as well as alanine, glutamate, aspartate, arginine and ornithine amino acids; p-values between 0.001 and p=0.05). The accumulation of metabolites in iPD fibroblasts was associated (and probably due) to the concomitant mitochondrial dysfunction observed at enzymatic, oxidative, respiratory and morphologic level. Metabolic and mitochondrial plasticity of controls was not observed in iPD fibroblasts, that were unable to adapt to different glucose conditions. Impaired metabolism and mitochondrial activity in iPD may limit energy supply for cell survival. Moreover, deficient plasticity to adapt to disrupted glucose balance characteristic of T2DM may underlay the comorbidity between both diseases. Conclusions:Fibroblasts form iPD patients showed mitochondrial impairment resulting in the accumulation of organic and amino acids related to mitochondrial metabolism, especially when exposed to high glucose condition. Mitochondrial and metabolic defects down warding cell plasticity to adapt to changing glucose bioavailability may explain the comorbidity between iPD and T2DM.