bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Family Presence on Pain and Anxiety Levels among Patients during Invasive Nursing Procedures in an Emergency Department
Authors: Gheshlaghi Parvin Abdi , Farahani Ziba Barzabadi , Anboohi Sima Zohari , Nasiri Malihe , Ziapour Arash , Garosi Vahid Hatami 
Published: March 2020

Abstract
Background: It is widely accepted that pain is the most common complaint during invasive nursing procedures, which causes anxiety in patients. The purpose of this study was to determine the effect of family presence on the level of pain and anxiety of patients during invasive nursing procedures in emergency department.Methods: The present non-randomized controlled clinical trial was conducted on 70 patients referred to emergency department at selected hospital affiliated to Kermanshah University of Medical Sciences, Iran, in 2018, who were selected by convenience sampling method and then randomly assigned into two groups of intervention (even days) and control (odd days). The invasive nursing procedure was performed for the intervention group in the family presence for physical and psychological support and for the control group without the family presence. Data collection tools were the Spielberger State-Trait Anxiety Inventory (STAI) and the Visual Analogue Scale (VAS). SPSS version 23 software was used to compare the mean scores of pain and anxiety using independent t-test.Results: The mean pain score after the invasive procedure had no significant difference between the intervention group (3.9±1.5) and the control group (4.7±1.9) (P=0.073). In the intervention group, the mean score of anxiety after invasive procedure was significantly lower than before the invasive procedure (P=0.028), whereas the control group showed no change (P=0.556).Conclusion: The family presence during the invasive nursing procedures reduced the anxiety of patients but had no effect on their pain. Emergency nurses can take advantage of family presence during invasive procedures as a non-pharmacological intervention to reduce patients' anxiety.
Motivational climate of group exercise sessions in nursing homes
Authors: Charles Alexia , Buckinx Fanny , Mouton Alexandre , Reginster Jean-Yves , Bruyère Olivier 
Published: March 2020

Abstract
BackgroundMotivational climate in exercise group environments would have an impact on adherence, effort and enjoyment. We examined the motivational climate among nursing home residents who were involved in group exercise sessions.Methods This cross-sectional study was conducted in 10 nursing homes of Liège area that offer group exercise sessions. Sociodemographic data (age, sex, body mass index), cognitive status (by the Mini Mental State Examination) and independence in activities of daily living (by the Katz Scale) were retrieved in the medical records. The “Abbreviated-Perceived Motivational Climate in Exercise Questionnaire” was translated into French and then administered face to face with a clinical researcher. This is composed of 6 ego-involving climate items (corresponding to rivalry, comparison and favoritism) and 6 task-involving climate items (corresponding to valorization, individual efforts, self-improvement and cooperation). Each item is ranged on a 5-point Likert scale ranging from 1 (not at all focused on ego or task) to 5 (totally focused on ego or task). Each subscale has a total score expressed as an average.ResultsA total of 102 subjects of exercise group sessions were included (84.3 ± 7.7 years and 83 (81.4%) women). The mean score of task-involving and ego-evolving motivational climate was respectively 3.57 (SD= 0.67) and 1.52 (SD=0.49), suggesting that the motivational climate was more focused on the task-involving climate than on ego-involving climate. Some items results were of particular interest: 55.9% of the respondents found that the instructor doesn’t remark/reward when they try hard, 63.7% said that the instructor doesn’t encourage mutual aid and 38.2% found that instructor doesn’t encourage to do new exercises.ConclusionsParticipants tended to perceive motivational climate as more task-involving than ego-involving. The absence of individual positive feedback, new exercises and mutual aid were also highlighted.
Screening of Concentration and Antimicrobial Effectiveness of Antimicrobial Preservative in Betastatin Besylate Nasal Spray
Authors: yanhong sun , Wang Yin-huan , Zhou Jian-ming , Zhou Qing-xue , Dong Shi-lei 
Published: March 2020

Abstract
OBJECTIVE To explore the optimal concentration and antimicrobial effectiveness of antimicrobial preservative in betastatin besylate nasal spray. METHODS By using Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candia albicans, and Aspergillus niger as test strains, the antimicrobial effectiveness of betastatin besylate nasal spray containing different concentrations of antimicrobial preservative (0.02%, 0.0125%, and 0.005% benzalkonium chloride, respectively) was determined by using bacteriostatic effect test (Chinese Pharmacopoeia, 2015 edition).RESULTS The antimicrobial effectiveness of betastatin besylate nasal spray containing 0.02% and 0.0125% benzalkonium chloride, respectively, complied with the regulations of Chinese Pharmacopoeia (2015 Edition) against five test strains. However, the antimicrobial effectiveness of betastatin besylate nasal spray containing 0.005% benzalkonium chloride against P. aeruginosa did not meet the requirements of Chinese Pharmacopoeia.CONCLUSION Benzalkonium chloride at a concentration of 0.125% can be used as an added antimicrobial preservative in betastatin besylate nasal spray.
Liquiritin represses proliferation , migration and invasion of colorectal cancer cells through inhibition of the miR-671/HOXB3 signaling pathway
Authors: Yang Liu , Zhang Yinan , Bao Jun , Feng Ji-Feng 
Published: March 2020

Abstract
Background: Colorectal cancer is a common malignant tumor and ranks third in cancer-related deaths. Considering that the side-effects of current therapies limit the clinical effectiveness, exploring new anti-colorectal cancer drugs from natural products is critical for the treatment of colorectal cancer. Among these drugs, liquiritin is an active component of the traditional Chinese herb Glycyrrhiza Radix and has been found to possess powerful anti-inflammatory and anti-tumor abilities. However, the direct molecular target of liquiritin remains unknown. Therefore, the aim of the present study was to identify potential molecular target of liquiritin to mediate its anti-colorectal cancer effect. Methods: The function of liquiritin in cell proliferation, apoptosis, migration and invasion was estimated by CCK-8 assay, colony formation, EdU assay, flow cytometry analysis, TUNEL assay, wound healing assay and transwell assay, respectively. Animal experiment was carried out to further confirm the role of liquiritin in vivo. H&E staining analysis, TUNEL staining and immunohistochemistry (IHC) assay were adopted for histological analysis. The mechanism research was conducted with RT-qPCR, western blot and luciferase report assay. Results: In this study, we found that liquiritin significantly inhibited the proliferation, migration, invasion and EMT processes of SW480 and HT-29 cells in a dose-dependent manner. MiRNAs have been extensively identified as drug targets in various studies. However, the miRNAs functioning as the direct targets of liquiritin remain unknown. In our study, we tested 8 potential pathogenic miRNAs screened from colorectal cancer patients, and we found that only the expression level of miR-671 was diminished due to liquiritin treatment. More importantly, overexpression of miR-671 partially abrogated the anti-tumor effects of liquiritin on colorectal cancer. Conclusions: Collectively, our findings demonstrated that liquiritin exhibits great potential in the treatment of colorectal cancer through regulation of miR-671.
Responsiveness and minimal clinically important difference of the EQ-5D-5L in cervical intraepithelial neoplasia: a longitudinal study
Authors: Hu Xin , Jing Mingxia , Zhang Mei , Yang Ping , Yan Xiaolong 
Published: March 2020

Abstract
Background: With the application of the EQ-5D-5L in clinic, whether the questionnaire scores can responsive to changes in patients' health and how much changes in questionnaire scores represents the real health change are gradually concerned. So the aims of the study were to assess the responsiveness and estimate MCID of the EQ-5D-5L in surgically treated CIN patients, and to analyze the relationship between MCID and MDC.Methods: This is a longitudinal observational study. Participants were CIN patients from the gynecology inpatient department of a Grade-A Tertiary Hospital in Shihezi, Xinjiang, China, which completed the EQ-5D-5L and the GRCQ at baseline and 1 month after the surgery. Wilcoxon signed-rank test was used to compare scores of the EQ-5D-5L before and after treatment. We calculated ES and SRM to assess the responsiveness quantitatively. Distribution-based, anchor-based and instrument-defined methods were used to estimate MCID. And ratios of MCID to MDC at individual and group levels were calculated. Results: 50 CIN patients completed follow-up investigate with mean (SD): age 44.76 (8.72) years; follow-up time 32.28 (1.43) days. The UI and EQ VAS of the EQ-5D-5L improved by 0.025 and 6.92 (all p <0.05) at follow-up compared baseline, respectively. ES and SRM of UI were 0.47 and 0.42, indicating small responsiveness, while ES and SRM of EQ VAS were 0.50 and 0.49, indicating small to medium responsiveness. The average (range) of MCIDs for UI and EQ VAS were 0.039 (0.023, 0.064) and 5.35 (3.12, 6.99), which can only be used for determing whether patients have experienced clinically meaningful improvement in health at group level. Conclusion: The EQ-5D-5L has only small responsiveness in patients with CIN after surgery, and MCIDs developed in this study can be used for health assessment at group level. Further study is needed on MCID that can be used at individual level. Keywords Responsiveness; MCID; EQ-5D-5L; CIN; MDC
A New Surgical Technique of Combination Retroperitoneal with Transperitoneal Laparoscopic Nephroureterectomy in a Single Position and Comparative Outcomes
Authors: Song Liming , Wang Wenkuan , Zhao Qinxin , Wen Yuhong , Zhou Xiaoguang , Han Hu , Zhang Xiaodong 
Published: March 2020

Abstract
Background The traditional surgical treatment for upper urinary tract urothelial carcinoma (UTUC) is time-consuming owing to changing the surgical position and larger surgical trauma because of open surgery in handling the distal ureter. Therefore, we created a new surgical technique of combination retroperitoneal with transperitoneal (CRT) laparoscopic nephroureterectomy (LNU) in a single position and to reported our early outcomes. Methods From January 2017 to October 2018, a total of 45 patients underwent LNU by a single surgeon at our department, of whom 25 patients underwent standard technique and 20 patients underwent CRT technique. Relevant clinical data were collected including each patient’s characteristics, surgical outcomes, and follow-up results. A comparative analysis between standard LNU cases and CRT LNU cases was performed. Results LNU was performed successfully on all 45 patients. There was no significant difference in patients’ characteristics. Compared to standard group, patients in CRT group had shorter operative time, less estimated blood loss (EBL), lower visual analogue scale (VAS) pain score and less scar ( P <0.001). The mean time of surgical drain stay decreased from 4.2d to 2.8d and median hospital stay after surgery decreased from 5d to 3d ( P <0.001). The complication rates did not show statistic difference between the two groups within the first 30 days postoperatively ( P =0.258). For the long-term complications, the incidence of abdomen bulge or incisional hernia in CRT group was less than that in the standard group ( P <0.001). Conclusion The CRT technique, which combines both the advantages of retroperitoneal and transperitoneal approaches, is a more minimally invasive, simplified and effective way to perform the LNU.
Evaluation of PTPRT Mutations as Biomarkers for Cancer Metastasis Across Multiple Cancer Types
Authors: chen chao , Zhang Xiuqing 
Published: March 2020

Abstract
Cancer metastasis is the main cause of cancer-related death, but the mechanism of metastasis is still unclear, and there is lack of metastasis markers. PTPRT is a protein coding gene which may be involved in both signal transduction and cellular adhesion, it is also known as a tumor suppressor gene that inhibits cell malignant proliferation by inhibiting the STAT3 pathway[1-3]. Recent studies have reported that PTPRT may be involved in the early metastasis of colorectal cancer, but to our knowledge, no comprehensive study has yet revealed a link between PTPRT and metastasis in other types of cancer[4, 5]. In this study, we found that mutations in PTPRT were a potential biomarker of cancer metastasis in multiple cancers through a combined analysis using a large data set.
Inhibitory effects of iron depletion plus eribulin on the breast cancer microenvironment
Authors: Goto Wataru , Kashiwagi Shinichiro , Asano Yuka , Takada Koji , Morisaki Tamami , Takahashi Katsuyuki , Fujita Hisakazu , Shibutani Masatsune , Amano Ryosuke , Takashima Tsutomu , Tomita Shuhei , Hirakawa Kosei , Ohira Masaichi 
Published: March 2020

Abstract
Background Iron is required for the proliferation of cancer cells, and its depletion suppresses tumor growth. Eribulin mesylate (eribulin), a non-taxane microtubule inhibitor, disrupts the tumor microenvironment via vascular remodeling and obstruction of the epithelial-mesenchymal transition (EMT). Herein, we investigated the effects of the iron chelator deferoxamine (DFO) on tumor-related properties of breast cancer cells and the effects of DFO plus eribulin on tumor growth in vivo . Methods A triple-negative breast cancer (TNBC) cell line (MDA-MB-231) was used in our study. Cell proliferation, cell migration, cell cycle position, and gene expression were analyzed via MTT assays, wound-healing assays, flow cytometry, and quantitative real-time-polymerase chain reaction, respectively. For the in vivo experiments, mice with MDA-MB-231 xenografts were treated with the inhibitors, alone or together, and tumor volume was determined. Results DFO inhibited breast cancer cell proliferation and migration and decreased the proportion of S-phase cells. Conversely, it induced hypoxia, angiogenesis, EMT, and immune checkpoints, as determined by quantifying the expression of marker mRNAs. Eribulin suppressed the expression of the EMT marker mRNAs in the presence of DFO. DFO plus eribulin inhibited tumor growth in vivo to a greater extent than did either inhibitor alone. Conclusions Although DFO induces oncogenic events (hypoxia, angiogenesis, EMT, and immune checkpoints), it may be an effective treatment for breast cancer when administered in combination with eribulin.
The imaging features and clinical associations of a novel tau PET tracer - 18F-APN1607(18F-PM-PBB3) in Alzheimer’s disease
Authors: Hsu Jung Lung , Lin Kun-Ju , Hsiao Ing-Tsung , Huang Kuo-Lun , Liu Chi-Hung , Wu Hsiu-Chuan , Weng Yi-Ching , Huang Chu-Yun , Chang Chiung-Chih , Yen Tzu-Chen , Higuchi Makoto , Jang Ming-Kuei , Huang Chin-Chang 
Published: March 2020

Abstract
Background: In vivo tau positron emission tomography (PET) imaging could help clarify the spatial distribution of tau deposition in Alzheimer’s disease (AD) and aid in the differential diagnosis of tauopathies. To date, there have been no in vivo 18 F-APN1607 tau PET studies in patients with AD.Methods: We applied tau tracer in twelve normal controls (NCs) and ten patients in the mild to moderate stage of probable AD. Detailed clinical information, cognitive measurements and disease severity were documented. Regional standardized uptake value ratios (SUVRs) from 18 F-AV-45 (florbetapir), 18 F-APN1607 PET images and regional gray matter (GM) atrophic ratios were calculated for further analysis.Results: Quantitative analyses showed significantly elevated SUVRs in the frontal, temporal, parietal, occipital lobes, anterior and posterior cingulate gyri, precuneus, and parahippocampal region (all ps < 0.01) with medium to large effect sizes (0.44 - 0.75). The SUVRs from 18 F-APN1607 PET imaging showed significant correlations with the ADAS-cog scores (all ps < 0.01) and strong correlation coefficients (R squared ranged from 0.54 to 0.68), even adjusted for age and gender effects. Finally, the SUVRs from 18 F-APN1607 PET imaging of the parahippocampal region showed rapid saturation as the ADAS-cog scores increased, and the SUVRs of the posterior cingulate gyrus and the temporal, frontal, parietal and occipital regions slowly increased. The combined SUVRs from 18 F-AV-45 PET, 18 F-APN1607 PET and regional GM atrophic ratio showed that uptake associated with the amyloid burden rapidly increased and reach a plateau, whereas uptake associated with tau depositions increased slowly and finally followed by regional GM atrophic ratios in most regions as the ADAS-cog scores increased. However, different regions exhibited various combinations of these patterns.Conclusions: Our findings suggest that the 18 F-APN1607 tau tracer showed a clear background without significant uptake in the basal ganglia or midbrain. Uptake of this tracer correlated well with cognitive changes and demonstrated the spatial pattern of amyloid, tau deposition and GM atrophy in the progression of AD. Thus, the regional base of dynamic biomarker changes was observed in the current study.Trial registration: registration number (NCT03625128), date of registration( August 10, 2018), retrospectively registered.
Efficacy of Chloroquine and Lopinavir/Ritonavir in mild/general COVID-2019: a prospective, open-label, multicenter randomized controlled clinical study
Authors: Liu Xi , Chen Huili , Shang Yuqi , Zhu Hongqiong , Chen Gongqi , Chen Yuanli , Liu Shaoxuan , Zhou Yaoyong , Huang Mingxing , Hong Zhongsi , Xia Jinyu 
Published: March 2020

Abstract
Background The outbreak of novel coronavirus pneumonia is very serious, and no effective antiviral treatment has been confirmed. The fresh drug research and development cycle is too long to meet clinical emergency needs, and "old drugs and brand new applications" have a huge therapeutic potential. During our previous treatment, we found that the lopinavir/ritonavir treatment recommended in the Fifth edition of the treatment plan had little effect. Earlier studies have shown that chloroquine can inhibit coronavirus replication through multiple mechanisms. Our previous use of chloroquine to treat patients with SARS-CoV-2(novel coronavirus)-infected pneumonia has a higher negative rate of nucleic acid in throat swabs within 5 days after administration than that using lopinavir/ritonavir. However, the half-life and side effects of chloroquine vary greatly among individuals. Methods/design We plan to conduct a prospective, open-label, multicenter randomized controlled, comprehensive treatment clinical study. The study consisted of three phases: a screening period of 1-110 days, a treatment period of no more than 28 days, and a follow-up period of 1 month. Participants will be assessed at baseline and on days 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 14, 21, and 28 after the intervention begins. In this study, chloroquine and lopinavir/ritonavir tablets were used to treat patients with eligible novel coronavirus pneumonia diagnosed at various centers between February 12, 2020 and May 31, 2020. The efficacy and safety of chloroquine and lopinavir/ritonavir are to be evaluated. At the same time, explore the correlation between patient genetic polymorphisms and chloroquine steady-state concentration, therapeutic effects and adverse reactions in the body. It is an anti-virus for pneumonitis caused by novel coronavirus. The optimization and update of the antiviral treatment plan provides evidence-based evidence. Disscussion Our study is a prospective, open-label, multicenter randomized controlled, comprehensive treatment clinical study to evaluate the efficacy and safety of chloroquine phosphate and lopinavir/ritonavir in patients with mild/general COVID-2019. The results of this study will provide valuable clinical evidence for the treatment of novel coronavirus pneumonia.
Elevated serum levels of galectin-3 binding protein is associated with insulin resistance in non-diabeteic women after menopause
Authors: Chen Yun , Das Sayantana , Zhuo Guangchao , Cai Hong 
Published: March 2020

Abstract
Background Galectin-3 binding protein (GAL-3BP) is one of the major fucosylated glycoprotein family members, and is implicated in nonalcoholic fatty liver disease, lipid dysfunctions and coronary artery disease recently. Here we analyzed the serum concentrations of GAL-3BP in postmenopausal women, to evaluate the association of circulating GAL-3BP and insulin resistance in female after menopause. Method We evaluated serum levels of Gal-3BP in sixty-two non-diabetic women in menopausal status for at least one year. The clinical features, biochemistry profiles and homeostasis model assessment of insulin resistance (HOMA-IR) were obtained routinely. Results Gal-3BP levels increased in women with higher HOMA-IR values and was positively correlated to HOMA-IR. It was also an independent risk factors for HOMA-IR and showed the most influence on HOMA-IR comparing to FPG, TG, age and BMI. The cut-off value of serum Gal-3BP level was 2234.3150 ng/ml with the area under the ROC curve (AUC) 0.68 (HOMA-IR 1.5), 0.81 (HOMA-IR 2.0) and 0.93 (HOMA-IR 2.5). Conclusions Serum levels of Gal-3BP is associated with impaired insulin sensitivity in non-diabetic menopausal women.
The burden and outcome of stroke in young adults in a Tanzanian Tertiary Hospital: A Comparison with older Adults
Authors: Matuja Sarah Shali , Munseri Patricia , Khanbhai Khuzeima 
Published: March 2020

Abstract
Background: Stroke burden in young adults is growing and is associated with unique risk factors and devastating outcomes. We aimed at describing the magnitude, risk factors and outcomes of first ever stroke in young adults ≤45 years compared to older adults >45 years. Methods: Participants were patients admitted with World Health Organization clinical criteria for stroke at a tertiary hospital in Tanzania. The National Institute of Health Stroke Scale and Modified Rankin Scale were used to assess for stroke severity and outcomes respectively. Kaplan-Meier analysis was used to estimate for survival. Results: We enrolled 369 first ever stroke participants out of 1403 medical admissions over 8 months’ duration. The overall stroke prevalence was 369 (26.3%) and was 123/484 {(25.4%) [95% CI 21.5% - 29.3%]} and 246/919 {(26.8%) [95% CI 23.9% - 29.6%]} for young and old respectively. Hemorrhagic stroke occurred in 47 (42.3%) vs 62 (27.2%) for the young and old respectively p=0.005. Factors associated with stroke in the young were: a new diagnosis of hypertension 33 (26.8%) vs 23 (9.3%) p<0.001, HIV infection 12 (9.8%) vs 7 (2.8%) p=0.005, use of hormonal contraception in females 33 (48.5%) vs 13 (9.4%) p<0.001, elevated serum low density lipoproteins 28 (27.7%) vs 29 (16.4%) p=0.024, hypercholesteremia 34 (31.2%) vs 40 (20.2%), p=0.031, sickle cell disease 11 (9.7%) vs 9 (4.2%) p=0.047 and thrombocytosis 12 (16.9%) vs 8 (5.6%) p=0.007. The median time for seeking health care was 2 days from stroke symptom onset. The overall 30-day fatality rate was 215 (61.3%) and 57 (49.1%) vs 158 (67.2%) in the young and old respectively. Conclusions: There is a high prevalence of stroke in young adults that is coupled with a high 30day fatality rate. Screening and treatment of hypertension is key in prevention of stroke in the young.