bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Adjuvanted Recombinant Zoster Vaccine in Adults Aged ≥65 Years Previously Vaccinated With a Live-Attenuated Herpes Zoster Vaccine
Authors: Dagnew Alemnew F , Klein Nicola P , Hervé Caroline , Kalema George , Di Paolo Emmanuel , Peterson James , Salaun Bruno , Schuind Anne 
Published: February 2020

Abstract

Background
Efficacy of the live-attenuated herpes zoster (HZ) vaccine (ZVL) wanes substantially over time. We evaluated immunogenicity and safety of the adjuvanted recombinant zoster vaccine (RZV) in previous ZVL recipients.

Methods
Adults aged ≥65 years who were previously vaccinated with ZVL ≥5 years earlier (n = 215) were group-matched with ZVL-naive individuals (n = 215) and vaccinated with RZV. Glycoprotein E (gE)–specific humoral and cell-mediated immune responses and the correlation between them, polyfunctional gE-specific CD4 T-cell responses, safety, and confirmed HZ cases were assessed.

Results
Through 12 months after dose 2, anti-gE antibody concentrations, gE-specific CD4 T-cell frequencies, and activation marker profiles were similar between groups. Safety outcomes were also similar. No HZ episodes were confirmed.

Conclusions
RZV induced strong humoral and polyfunctional cell-mediated immune responses that persisted above prevaccination levels through 1 year after dose 2 in adults aged ≥65 years irrespective of previous ZVL vaccination. The RZV safety profile was not affected.

Clinical Trials Registration
NCT02581410.
JUND-dependent up-regulation of HMOX1 is associated with cisplatin resistance in muscle-invasive bladder cancer
Authors: Peng Ye , Chen Yongjie , Chen Shiwei , Wang Jiaolian , Jiang Cheng , Hou Wugang , Xu Chun 
Published: February 2020

Abstract
The standard-of-care for metastatic muscle-invasive bladder cancer (MIBC) is platinum-based chemotherapy regimens. Acquired resistance that occurs frequently through unidentified mechanisms, however, remains the major obstacle for implementing therapeutic effectiveness. Here, using data mining and analysis on clinical samples, we show that expression of JUND, a core component of activator protein-1 family, was significantly induced in cisplatin (CDDP)-resistant MIBC. Accumulation of nuclear JUND was associated with low post-chemotherapy survival in MIBC patients. In both genetically engineered cell models and murine xenograft models, we provided evidence that bladder cancer (BC) cells with excessive JUND expression were less responsive to CDDP treatment. This CDDP resistance was further demonstrated to be mediated, at least in part, by transactivation of HMOX1 [the gene encoding heme oxygenase-1 (HO-1)], one of the most important antioxidant signalling pathways of cell adaptation to stress. One mutation within the HMOX1 promoter successfully abolished oxidative stress-enhanced and JUND-driven HMOX1 promoter activation, suggesting that this unique site synergized for maximal HO-1 induction in CDDP-challenged BC cells. Overall, our data highlight an indispensible role of JUND, both as a target as a modifier of the oxidative stress signalling, in conferring an adaptive response during the pathogenesis of CDDP resistance in MIBC.
Growth, mortality, ovarian maturity, and exploitation of the Guinea shrimp, Holthuispenaeopsis atlantica (Balss, 1914) (Decapoda: Dendrobranchiata: Penaeidae) in inshore waters of Ghana, West Africa
Authors: Okpei Paulina , Aggrey-Fynn Joseph , Okyere Isaac 
Published: February 2020

AbstractThe Guinea shrimp, Holthuispenaeopsis (= Parapenaeopsis) atlantica (Balss, 1914) is exploited by traditional and commercial fisheries in the Gulf of Guinea, West Africa. We assessed growth, mortality, ovarian maturity, and exploitation of the population of the species in inshore waters of Ghana as a contribution of filling the knowledge gap on the species. Samples were obtained from fish landing sites from three different coastal towns in Ghana from October, 2017 to May, 2018. Length-frequency data were used to estimate growth, mortality and exploitation ratio. The modal class for males was 6.0–6.9 cm total length (TL) and 9.0–9.9 cm TL in females. The length and weight relationship established that growth in both sexes was negatively allometric. The estimated growth parameters from the length frequency data fitted with the von Bertalanffy growth function were asymptotic length (L∞) of 10.08 cm for males and 15.4 cm for females. The growth constant (K) was calculated as 0.99 yr–1 for males and 0.89 yr–1 for females. The mean length-at-first capture (Lc) was found to be lower than the mean length at sexual maturity (Lm). The sex ratio showed a preponderance of females over males throughout the period, with 76.21% females. The total mortality rate (Z) for males was more than for females. The estimated natural mortality was 2.32 yr−1 for males and 1.93 yr−1 for females (M) whiles ﬁshing mortality (F) rate was 1.22 yr−1 for males and 1.46 yr−1 for females. The exploitation ratio showed the stocks were not over-exploited. The estimated population parameters of the species obtained should be significant for the sustainable management of this resource.
Mortality of Different Stages of Plodia interpunctella (Lepidoptera: Pyralidae) at Three Temperatures in Controlled Atmosphere of High Nitrogen
Authors: Huang Yilin , Wang Dianxuan , Jian Fuji , Jayas Digvir S , Chen Chuqiao , Wang Dingyue 
Published: February 2020

AbstractThe lethal exposure time to controlled atmospheres of high nitrogen at stored grain temperatures is an important information for control of stored-product insects. The mortality of 1-d-old egg, 1-wk-old (first or second instar) larva, 3-wk-old (fourth or fifth instar) larva, and 1-d-old pupa of Plodia interpunctella (Hübner) was determined at 18 ± 1, 23 ± 1, and 28 ± 1°C in 98% N2 mixed with air. At 18°C, the lethal exposure times to achieve 100% mortality were 12.7 ± 0.7, 16.3 ± 0.3, 19.7 ± 0.7, and 14.7 ± 0.7 d for 1-d-old egg, 1-wk-old larva, 3-wk-old larva, and 1-d-old pupa, respectively. Temperature had significant effect on the lethal exposure time, and increase of the temperature significantly decreased the lethal exposure time. The order of the insect stages from the highest to lowest for LT50 values was follows: 3-wk-old larva &gt; 1-wk-old larva &gt; 1-d-old pupa ≥ 1-d-old egg. The minimum lethal exposure times required to kill all stages of P. interpunctella were about 20, 16, and 12 d at 18, 23, and 28°C, respectively.
A Real-Time PCR Assay for Rapid Identification of Tuta absoluta (Lepidoptera: Gelechiidae)
Authors: Zink Frida A , Tembrock Luke R , Timm Alicia E , Gilligan Todd M 
Published: February 2020

AbstractThe tomato leafminer, Tuta absoluta (Meyrick), is a highly destructive pest of tomatoes, causing damage to leaves, stalks, buds, and fruits. Native to South America, T. absoluta is now found throughout Europe, South Asia, Africa, parts of Central America, and the Caribbean. Adults are small, with a wingspan of approximately one cm and lack distinctive markings, making morphological identification difficult. Larvae are also difficult to identify and resemble those of many other gelechiids. Due to the extensive time spent and expertise required for morphological identification, and the imminent threat to the North American tomato crop, we have developed a rapid molecular test for discriminating individual specimens of T. absoluta using a probe-based real-time polymerase chain reaction (PCR) assay. The assay is able to quickly distinguish T. absoluta from similar-sized moth specimens that are attracted to T. absoluta pheromone lures in the United States and is also able to identify larvae of T. absoluta. Decreased identification time for this critical pest will lead to more rapid identification at ports of entry and allow for more efficient trap screening for domestic monitoring programs.
Effect of Treatment With 3-Octylthio-1,1,1-Trifluoropropan-2-One in the Diamondback Moth (Lepidoptera: Plutellidae) to the Toxicity of Diafenthiuron, Indoxacarb, and Bacillus thuringiensis
Authors: Huang Jingfei , Tian Sufen , Ren Ke , Chen Yong , Lin Shuo , Chen Yixin , Tian Houjun , Zhao Jianwei , Wang Changfang , Wei Hui , Gu Xiaojun 
Published: February 2020

Abstract
The diamondback moth, Plutella xylostella (L.), is a worldwide insect pest of cruciferous crops. Although insecticides have long been used for its control, diamondback moth rapidly evolves resistance to almost any insecticide. In insects, juvenile hormone (JH) is critically involved in almost all biological processes. The correct activity of JH depends on the precise regulation of its titer, and juvenile hormone esterase (JHE) is the key regulator. Thus, JH and JHE have become important targets for new insecticide development. Trifluoromethyl ketones are specific JHE inhibitors, among which 3-octylthio-1,1,1-trifluoropropan-2-one (OTFP) has the highest activity. The interaction effects between pretreatment with or combination of OTFP and the insecticides diafenthiuron, indoxacarb, and Bacillus thuringiensis (Bt) were investigated in diamondback moth larvae to determine OTFP’s potential as an insecticide synergist. In third-instar larvae, both pretreatment and combination treatment with OTFP decreased or antagonized the toxicities of diafenthiuron, indoxacarb, and Bt at all set concentrations. In fourth-instar larvae, combination treatment with OTFP decreased or antagonized the toxicities of diafenthiuron and indoxacarb at all set concentrations. However, it increased or synergized the toxicity of Bt at lower concentrations despite the limited effect at higher concentrations. Our results indicated that the effect of OTFP on the toxicities of insecticides varied with the type and concentration, larval stage, and treatment method. These findings contribute to the better use of OTFP in diamondback moth control.
Trunk Refugia: A Simple, Inexpensive Method for Sampling Tree Trunk Arthropods
Authors: Salman Ibrahim N A , Ferrante Marco , Möller Daniella M , Gavish-Regev Efrat , Lubin Yael 
Published: February 2020

AbstractTrees host a large share of the global arthropod diversity. Several methodologies have been described to sample arthropods from trees, ranging from active sampling techniques (e.g., visual searching, beating, or shaking the branches) to passive sampling devices. The majority of these collection techniques are destructive, and do not specifically target the tree trunk arthropod fauna. Here, we describe an alternative sampling method called trunk refugia (TR). TR are cylindrical shelters made of corrugated cardboard that can be secured to trees using string, and can remain exposed for varying time periods. These refugia are inexpensive, easy to use, and suitable to monitor a diverse array of insects and arachnids. Moreover, TR are nonlethal sampling tools, and allow collecting live individuals for behavioral studies or for rearing.
Regional-scale variation on Dromiciops gliroides occurrence, abundance, and activity patterns along a habitat disturbance gradient
Authors: Rodríguez-Gómez Gloria B , Fontúrbel Francisco E 
Published: February 2020

AbstractHabitat structure may have a significant influence on the occurrence, abundance, and activity patterns of forest mammals. However, anthropogenic habitat disturbance changes habitat structure, which may alter those patterns of activity. We assessed occurrence, relative abundance, and activity patterns of Dromiciops gliroides, an arboreal marsupial endemic to the temperate rainforests of southern South America, contrasting four forest conditions at a regional scale: old-growth, second-growth, and logged forests, and abandoned exotic plantations. We conducted a camera-trap assessment in two consecutive austral summers across most of the Chilean range of D. gliroides, and compared habitat structure along a disturbance gradient. All structural features assessed differed among forest conditions. Dromiciops gliroides was present in all forest conditions, but its abundance decreased and activity got narrower as disturbance increased, being significantly lower in the exotic plantations. Activity patterns were variable among forest conditions and months, and were significantly more restricted temporally at exotic plantations. Although D. gliroides is tolerant to habitat disturbance, we show that structural alteration results in lower abundances and narrower activity patterns.
Transcriptional analysis of arogenate dehydratase genes identifies a link between phenylalanine biosynthesis and lignin biosynthesis
Authors: El-Azaz Jorge , de la Torre Fernando , Pascual María Belén , Debille Sandrine , Canlet Francis , Harvengt Luc , Trontin Jean-François , Ávila Concepción , Cánovas Francisco M 
Published: February 2020

Abstract
Biogenesis of the secondary cell wall in trees involves the massive biosynthesis of the phenylalanine-derived polymer lignin. Arogenate dehydratase (ADT) catalyzes the last, and rate-limiting, step of the main pathway for phenylalanine biosynthesis. In this study, we found that transcript levels for several members of the large ADT gene family, including ADT-A and ADT-D, were enhanced in compression wood of maritime pine, a xylem tissue enriched in lignin. Transcriptomic analysis of maritime pine silenced for PpMYB8 revealed that this gene plays a critical role in coordinating the deposition of lignin with the biosynthesis of phenylalanine. Specifically, it was found that ADT-A and ADT-D were strongly down-regulated in PpMYB8-silenced plants and that they were transcriptionally regulated through direct interaction of this transcription factor with regulatory elements present in their promoters. Another transcription factor, PpHY5, exhibited an expression profile opposite to that of PpMYB8 and also interacted with specific regulatory elements of ADT-A and ADT-D genes, suggesting that it is involved in transcriptional regulation of phenylalanine biosynthesis. Taken together, our results reveal that PpMYB8 and PpHY5 are involved in the control of phenylalanine formation and its metabolic channeling for lignin biosynthesis and deposition during wood formation in maritime pine.
Discovery of high-quality daytime seeing windows at the Antarctic Taishan station
Authors: Tian Qiguo , Jiang Peng , Jin Xinmiao , Li Jialu , Pei Chong , Du Fujia , Li Zhengyang , Li Xiaoyan , Chen Hualin , Ji Tuo , Shi Xiheng , Zhang Shaohua , Yang Chenwei , Zhou Hongyan 
Published: February 2020

ABSTRACT
We report on the initial results of seeing measurements at the Antarctic Taishan Station (ATS) using a differential image motion monitor observed in the visible at a height of 2.5 m above the snow surface, during the site-testing experiments carried out on 2014 January 13 and 15. The median seeing was found to be 0.73 arcsec and the 25th and 75th percentiles of the seeing cumulative distribution were 0.59 and 0.87 arcsec, respectively, with a minimum of ∼0.5 arcsec at ∼16:00–19:00 local time (UTC + 5). Such a seeing minimum has a good temporal association with the refractive index structure constant ($C_n^2$) minimum simultaneously observed at ATS. Interestingly, both temporal windows of $C_n^2$ and seeing minimum coincide with those of minimum temperature gradients ($\rm \sim 0\,^{\circ }C\,m^{-1}$), we had more than one month of continuous measurements of Cn2 and temperature gradients between 2013 December 30 and 2014 February 10. This suggests that high-quality seeing windows might be opened at ATS with the potential for astronomical activities, especially for solar observations.
Properties of giant molecular clouds in the strongly barred galaxy NGC 1300
Authors: Maeda Fumiya , Ohta Kouji , Fujimoto Yusuke , Habe Asao 
Published: February 2020

ABSTRACTStar formation activity depends on galactic-scale environments. To understand the variations in star formation activity, comparing the properties of giant molecular clouds (GMCs) among environments with different star formation efficiency (SFE) is necessary. We thus focus on a strongly barred galaxy to investigate the impact of the galactic environment on the GMC properties, because the SFE is clearly lower in bar regions than in arm regions. In this paper, we present the 12CO(1 − 0) observations towards the western bar, arm, and bar-end regions of the strongly barred galaxy NGC 1300 with ALMA 12-m array at a high angular resolution of ∼40 pc. We detected GMCs associated with the dark lanes not only in the arm and bar-end regions but also in the bar region, where massive star formation is not seen. Using the CPROPS algorithm, we identified and characterized 233 GMCs across the observed regions. Based on the Kolmogorov–Smirnov test, we find that there is virtually no significant variations in GMC properties (e.g. radius, velocity dispersion, molecular gas mass, and virial parameter) among the bar, arm, and bar-end region. These results suggest that systematic differences in the physical properties of the GMCs are not the cause for SFE differences with environments, and that there should be other mechanisms which control the SFE of the GMCs such as fast cloud–cloud collisions in NGC 1300.
Monitoring the spatio-temporal organization and dynamics of the genome
Authors: Shaban Haitham A , Seeber Andrew 
Published: February 2020

Abstract
The spatio-temporal organization of chromatin in the eukaryotic cell nucleus is of vital importance for transcription, DNA replication and genome maintenance. Each of these activities is tightly regulated in both time and space. While we have a good understanding of chromatin organization in space, for example in fixed snapshots as a result of techniques like FISH and Hi-C, little is known about chromatin dynamics in living cells. The rapid development of flexible genomic loci imaging approaches can address fundamental questions on chromatin dynamics in a range of model organisms. Moreover, it is now possible to visualize not only single genomic loci but the whole genome simultaneously. These advances have opened many doors leading to insight into several nuclear processes including transcription and DNA repair. In this review, we discuss new chromatin imaging methods and how they have been applied to study transcription.
Exploring the Point-of-Sale Among Vape Shops Across the United States: Audits Integrating a Mystery Shopper Approach
Authors: Berg Carla J , Barker Dianne C , Meyers Christina , Weber Amber , Park Amy J , Patterson Akilah , Dorvil Sarah , Fairman Robert T , Huang Jidong , Sussman Steve , Livingston Melvin D , Wagener Theodore L , Hayes Rashelle B , Pulvers Kim , Getachew Betelihem , Schleicher Nina , Henriksen Lisa 
Published: February 2020

Abstract

Introduction
Vape shops represent prominent, unique retailers, subject to Food and Drug Administration (FDA) regulation in the United States.

Aims and Methods
This study assessed compliance of US vape shop retail marketing strategies with new regulations (eg, required age verification, prohibited free samples) and pre-implementation conditions for other regulations (eg, health warning labels on all nicotine products, required disclosures of e-liquid contents).

Results
95.0% of shops displayed minimum-age signage; however, mystery shoppers were asked for age verification at 35.6% upon entry and at 23.4% upon purchase. Although 85.5% of shops had some evidence of implementing FDA health warnings, 29.1% had signage indicating prohibited health claims, 16.3% offered free e-liquid samples, 27.4% had signage with cartoon imagery, and 33.3% were within two blocks of schools. All shops sold open-system devices, 64.8% sold closed-system devices, 68.2% sold their own brand of e-liquids, 42.5% sold e-liquids containing cannabidiol, 83.2% offered price promotions of some kind, and 89.9% had signage for product and price promotions.

Conclusions
Results indicated that most shops complied with some implementation of FDA health warnings and with free sampling bans and minimum-age signage. Other findings indicated concerns related to underage access, health claims, promotional strategies, and cannabidiol product offerings, which call for further FDA and state regulatory/enforcement efforts.
Temperature analysis of solar prominences by multi-wavelength observations
Authors: Okada Sanetaka , Ichimoto Kiyoshi , Machida Aki , Tokuda Satomi , Huang Yuwei , UeNo Satoru 
Published: February 2020

Abstract
The aim of this paper is to determine the kinetic temperature of solar prominences from widths of emission lines with a careful evaluation of its validity, and to investigate the spatial distribution of the non-thermal velocity and the kinetic temperature in prominences. Spectroscopic observations of prominences were carried out using the Domeless Solar Telescope at the Hida observatory in four emission lines, i.e., H$\alpha$$656.3\:$nm, H$\beta$$486.1\:$nm, Ca$^{+}$IR $854.2\:$nm, and Ca$^{+}$K $393.4\:$nm. We found systematic differences in temperature and its spatial distributions in prominences obtained from different pairs of spectral lines. The reason for the difference is that the intrinsic widths of emission lines determined by a single-slab model are different for optically thick and thin lines, and can be attributed to the assumption of a Gaussian profile of the non-thermal broadening in the single-slab model. With numerical experiments, we found that the observed differences among line pairs can be reproduced by a multi-slab model, in which each slab has different non-thermal broadening and line-of-sight velocity. The most accurate evaluation of the temperature is obtained with the pair of H$\beta$ and Ca$^{+}$IR in our selection. Based on these results, we found the typical kinetic temperature of prominences to be 8000–$12000\:$K, which is significantly higher than the temperature determined by radiative balance and suggests the presence of mechanical heating in prominences. Also suggested is the presence of positive spatial correlation between non-thermal velocity and temperature. We revisit past observational results on the prominence temperature and discuss the reason for discrepancies among them.
Quantitative Imaging Reveals Distinct Contributions of SnRK2 and ABI3 in Plasmodesmatal Permeability in Physcomitrella patens
Authors: Tomoi Takumi , Kawade Kensuke , Kitagawa Munenori , Sakata Yoichi , Tsukaya Hirokazu , Fujita Tomomichi 
Published: February 2020

AbstractCell-to-cell communication is tightly regulated in response to environmental stimuli in plants. We previously used a photoconvertible fluorescent protein Dendra2 as a model reporter to study this process. This experiment revealed that macromolecular trafficking between protonemal cells in Physcomitrella patens is suppressed in response to abscisic acid (ABA). However, it remains unknown which ABA signaling components contribute to this suppression and how. Here, we show that ABA signaling components SUCROSE NON-FERMENTING 1-RELATED PROTEIN KINASE 2 (PpSnRK2) and ABA INSENSITIVE 3 (PpABI3) play roles as an essential and promotive factor, respectively, in regulating ABA-induced suppression of Dendra2 diffusion between cells (ASD). Our quantitative imaging analysis revealed that disruption of PpSnRK2 resulted in defective ASD onset itself, whereas disruption of PpABI3 caused an 81-min delay in the initiation of ASD. Live-cell imaging of callose deposition using aniline blue staining showed that, despite this onset delay, callose deposition on cross walls remained constant in the PpABI3 disruptant, suggesting that PpABI3 facilitates ASD in a callose-independent manner. Given that ABA is an important phytohormone to cope with abiotic stresses, we further explored cellular physiological responses. We found that the acquisition of salt stress tolerance is promoted by PpABI3 in a quantitative manner similar to ASD. Our results suggest that PpABI3-mediated ABA signaling may effectively coordinate cell-to-cell communication during the acquisition of salt stress tolerance. This study will accelerate the quantitative study for ABA signaling mechanism and function in response to various abiotic stresses.