bluuebunny/crossref_metadata_2025_split huggingface dataset:
STUDI PENAMBAHAN SERAI (Cymbopogon citratus) DAN TEMU MANGGA (Curcuma mangga) TERHADAP KARAKTERISTIK FISIKOKIMIA DAN ORGANOLEPTIK MINUMAN SARI UMBI BIT (Beta vulagris L.)
Authors: Datuyanan Inten Sharon , Simanjuntak Bistok Hasiholan , Setiawan Andree Wijaya , Handoko Yoga Aji 
Published: October 2020

As food contains vitamin, mineral and antioxidant, beetroot (Beta vulgaris L.) had beneficial for health. Although beetroot contains a lot of nutritional value, beetroot is less liked by the public because of an earthy taste. For this reason, it needs innovation of the beetroot processing into products that can be accepted by the public, such as beverage fruit drink with food additives. In this study, beetroot juice with food additives, i.e. lemongrass, and turmeric mango was expected to improve taste and acceptable consumer. This study were aimed to analyze the effect of lemongrass and turmeric mango on physicochemical characteristics and determine the organoleptic quality of beetroot juice. This study used a Randomized Block Design (RBD) with the addition of lemongrass and turmeric mango respectively 1%, 3%, 5% concentration in the beetroot juice. Negative control used beetroot juice plain and the positive control used beet juice plus honey. Data were analyzed by variance (ANOVA), if the results obtained were significantly different, the DMRT continuing with 5% significant level. The results showed that the addition of lemongrass and turmeric mango with the concentration of each 1%, 3%, 5% effect on the physicochemical characteristics and organoleptic beet juice. The highest total dissolved solids was 6.92oBrix and the highest betacyanin content of 10.26 mg/L occurred at the addition of 5% turmeric mango. The highest pH value was shown in the negative control (beetroot juice plain) with an average of 6.52. The highest vitamin C content found in the addition of 1% lemongrass about 6.16 mg/100g. The results also showed that the organoleptic beet juice with the addition of 1% lemongrass and the positive control (beet juice plus honey) as a sample that it the most preferred by the panelists.
Keywords: beetroot juice, lemongrass, physical-chemistry, organoleptic, turmeric mango
A Treatment Pathway Variation for Chronic Prosthesis-Associated Infections
Authors: Brügger Jan , Saner Simon , Nötzli Hubert P. 
Published: October 2020

Background:
Periprosthetic joint infections (PJIs) are relatively rare but are on the rise because of the increasing total number of implantations performed. Treatment of PJI remains individualized and involves both surgical and medical treatment, with variations depending on the time of implantation, the duration and severity of the infection, tissue damage, and the underlying microorganism. In this case series study, we investigated clinical and functional outcomes of a variation of the Liestal algorithm in patients with PJI following total hip arthroplasty.

Methods:
This study included 32 patients (33 cases) who were treated for chronic PJI with 2-stage exchange using a cement spacer during the period of 2003 to 2014. In contrast to other treatment pathways, antibiotic therapy was targeted to the causative microorganism as early as possible despite the presence of a cement spacer. Second-look surgery was performed 4 days after removal of the primary implant and a 4-week antibiotic-free window was interposed before definitive reimplantation. Thereafter, antibiotic treatment continued for approximately 6 weeks. All patients were followed for a minimum of 2 years. Parameters investigated were the duration of infection-free survival, functional outcome, and epidemiological data.

Results:
At 2 years of follow-up and at the most recent follow-up (on average, 7 years after reimplantation), 100% of the patients were free of signs of infection, and the mean Harris hip score (HHS) was 89 at the latest follow-up.

Conclusions:
A meticulously performed 2-stage exchange for PJI with early targeted antibiotic treatment, second-look surgery, an antibiotic-free window before reimplantation, and antibiotic treatment post-reimplantation of medium duration is associated with excellent infection-related and good functional outcome after ≥2 years of follow-up even in cases of chronic PJI.

Level of Evidence:
Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Monitoring the Accuracy of Measurements the Load of Industrial Enterprises
Authors: Anishchenko V. A. , Pisaruk T. V. 
Published: October 2020

The normalized accuracy of the installed measuring instruments determines the scheduled measurement accuracy of the daily active load of an industrial enterprise. To maintain it during operation, syntactic and semantic methods can be used to control the reliability of load measurements. During syntactic control, the state of measuring instruments that collect and process information about the enterprise’s load is diagnosed. Semantic control is based on the use of probabilistic characteristics of the measured load. The condition necessary for semantic control is the presence of information redundancy about the values of the monitored load. It is possible to control the load limit values (settings) when a priori information is known about the lower and upper limits in which reliably measured load values in normal operation of the enterprise may be contained. The article considers control methods that use a posteriori redundant information about the dynamics of load alterations in various sections of the daily schedule. These methods include monitoring by the first increments of the load, which characterize the speed of its alterations, as well as control using linear and nonlinear extrapolations of the first increments. The results of the analysis of probabilistic characteristics of the daily active load of the enterprise and its first increments, which characterize the rate of load change, are presented. The effect of probabilistic characteristics of the first load increments on the boundaries of making a decision about the reliability of measurements is demonstrated. A comparative analysis of the effectiveness of reliability control methods based on the first load increments and their extrapolated values has been carried out on the example of measurements of daily active load schedules of the “Minsk Motor Plant” JSC.
Risky Sexual Behaviour among Male Immigration and Correctional Personnel in Kano State, Nigeria, 2018
Authors: Azeez Nureni , Usman Aishat Bukola , Stephen Adebowale Ayo , Jibo Abubakar Muhammad , Iliyasu Zubairu 
Published: October 2020

Abstract
BackgroundWorldwide, uniformed personnel have been recognized as a population at high risk for sexually transmitted infections because of their high mobility, power differentials and affluence. However, limited studies have been conducted on risky sexual behaviour (RSB) among male uniformed personnel in Nigeria. We conducted this study to determine the prevalence and identify the predictors of RSB among male immigration and correctional personnel in Kano State.MethodsWe conducted a cross-sectional study among 306 male immigration and correctional personnel using multi-stage sampling technique. A pre-tested self-administered structured questionnaire containing sociodemographic and variables associated with RSB was used for data collection. Indicators of RSB included transactional sexual intercourse, sexual intercourse with casual acquaintances and multiple non-regular sexual partners over the last 12 months. Data were analysed using descriptive statistics, Chi-square test and logistic regression (α=0.05).ResultsMean age of the respondents in the immigration and correctional services was 37.9±9.3 and 38.0±8.1 years respectively. Overall prevalence of RSB was 15.1% (9.2% and 20.9% among correctional and immigration workers respectively). The identified predictors of RSB were service group, marital status and attending club house prior to sexual intercourse. The likelihood of RSB was lower among the correctional personnel (aOR=0.38, CI=0.17-0.80) than the immigration officers. Being married is protective of RSB (aOR=0.38, CI=0.18-0.81) while attending club house prior to sexual intercourse predisposes the uniform personnel to RSB (aOR=3.90, CI=1.30-11.68). ConclusionsRisky sexual behaviour was highly prevalent among the investigated uniform men but was higher among immigration officers than the correctional personnel.
Post-transplant monitoring of lymphocyte counts predict the occurrence of CMV DNAemia in early phase of kidney transplantation
Authors: Feng Sujuan , Yang Jing , Zhang Xiaodong 
Published: October 2020

Abstract
Background The aim of this study was to assess whether monitoring of the number of lymphocytes in peripheral blood was helpful for evaluating the risk of cytomegalovirus (CMV) infection after kidney transplantation. Methods The total numbers of lymphocyte in peripheral blood were measured at baseline and posttransplant months 1, 3 and 6. Risk factors for DNAemia in KTRs were analyzed using univariate logistic regression analyses. Areas under receiver operating characteristic (ROC) curves were applied to assess the accuracy of lymphocyte counts for predicting CMV DNAemia. Results After follow-up 6 months, CMV replication was detected in 12 (31.6%) kidney transplant recipients (KTRs). The total lymphocyte counts were significantly decreased in KTRs with CMV DNAemia in 1, 3 and 6 months. There was a negative correlation between CMV copies and the lymphocyte counts in 1, 3 and 6 months post-transplantation, and the decrease of lymphocyte counts in the 6 months post-transplantation was the risk factor of CMV DNAemia in the KTR. Patients with lymphocyte counts 1.085×109 cells/L had higher cumulative incidence of CMV DNAemia.Conclusions The lymphocyte counts post kidney transplantation may be used as a simple and effective indicator for monitoring the CMV DNAemia status in KTR and for predicting the risk of CMV DNAemia.
Correlation analysis of oxygen consumptions between stepper and treadmill movements and implication for rehabilitation
Authors: Mukrikakkada Anwar Hussain , Arnold Graham , Abboud Rami , Wang Weijie 
Published: October 2020

Abstract
Background: Stepper movement is popular as a home-based exercise. However, it has not been fully investigated how much energy is consumed during movement. This study aimed to investigate oxygen consumption for stepper movements at different cadence levels and to look into whether there are correlations between stepper movement and treadmill walking. Methods: Twenty two healthy volunteers (10 males, 12 females, aged between 18 and 40) participated in the study. The participants randomly performed stepper movements and treadmill walking at three different cadences, a self-determined comfortable cadence, a 20% higher and a 20% lower ones than the comfortable one. Their oxygen consumption was measured using Oxycon mobile® system. A set of parameters, e.g. VO2, Metabolic unit, Energy expenditure, etc were obtained. Statistical analysis was carried out to investigate correlations between stepper and treadmill movements in terms of oxygen consumptions. Results: Strong correlations were found between two types of movements, most of metabolic parameters had correlation coefficients ranged proximately between 0.5 to 0.8, p<0.001, e.g. 0.789 for VO2, 0.790 for energy consumption, 0.826 for ventilation, all p<0.001. In the conformable cadence level, VO2 for stepper movements was 826.49±56.02 (ml/min) while VO2 for treadmill walking was 787.16±56.02. Usually, stepper movements have oxygen consumption similar to or slightly higher than that in treadmill walking.Conclusions: This study indicates that stepper movement has similar oxygen consumption level to treadmill at a comfortable cadence level and at a 20% higher than the comfortable one, but stepper movement consumes more energy than treadmill walking by approximately 10% at lower cadence (i.e. 20% lower than the comfortable one). The study shows that the stepper movements is highly correlated with treadmill walking in terms of oxygen consumption, and thus stepper movement data provided may be used to predict the treadmill data in rehabilitation in the future.
PRODUCTION OF BIODIESEL FROM MIXED CASTOR SEED AND MICROALGAL OILS: CHARACTERIZATION AND OPTIMIZATION STUDIES
Authors: Lemma Dejene Beyene , Abagisa Mohammedsani Abdulkadir , Kebede Adisu Befekadu 
Published: October 2020

Abstract
In order to lower the high prices of individual feedstock, extend the life of a more limited feedstock and to improve the fuel properties of biodiesel it imperative to optimize process parameters for biodiesel derived from mixed feedstocks. Samples of castor seeds were collected from Jimma zone, southwest Ethiopia while sample of wet microalgal biomass was obtained from wastewater stabilization ponds using a 60µm filter screen. The castor seeds and algal biomass were sun-dried before further dried at 80 0 C in an oven and ground to pastes. Oils were extracted from dried and milled castor seeds and micro-algae pastes with a Soxhlet apparatus using methanol. The extracted oil was purified and characterized before converted to biodiesel. A transesterification process designed using Response Surface Methodology (RSM) based on central composite design (CCD) experimental design was used to optimize the biodiesel production process parameters from mixed oil using alkaline catalyst. The Design Expert® 12 software was used to analysis experimental results. The effects of catalyst concentration, ethanol to mixed oil molar ratio and reaction temperature on the biodiesel yield were investigated using the experimental results. Accordingly, the optimum conditions for biodiesel production from mixed oil were a catalyst concentration of 1.23% w.t of the oil, alcohol to mixed oil molar ratio of 5.94 :1 (v/v) and reaction temperature of 51.30 0 C. The yield of biodiesel under these conditions was 93.88% . Experiment was conducted under the specified optimum conditions to validate the result predicted by the software. The yield of biodiesel from the experiment was 93.36 % which is very close to the value predicted by the software. The fatty acid composition of the biodiesel from mixed oil was analyzed using Gas chromatograph. The various fuel properties of biodiesel were determined using standard methods and results were compared with ASTM D6751 and EN 14214 standards. The physicochemical properties fulfill both standards.
The Association Between Living Environmental Factors and Adolescents’ Body Weight
Authors: HUANG SIYI , Sha Sha , Du Wei , Zhang Hanwen , Wu Xinyi , Jiang Chongmin , Zhao Yan , Yang Jie 
Published: October 2020

Abstract
BackgroundThe effect of living environment on public health has received increasingly scholarly attention. This study aims to explore the relationship between adolescents’ body weight and their living environmental factors. MethodsThis cross-sectional study comprised 1362 middle-school students from Nanjing and 826 from Changzhou in China. We further collected information on living environmental factors based on their home address and ran a univariate logistic analysis for each variable to explore potential correlations.ResultsApproximately 25% (n=303) of students from Nanjing and 26% (n=205) of students from Changzhou were excessively overweight. In Nanjing, students’ BMI (Body Mass Index) showed a strong negative correlation with the number of sports venues in their neighbourhood (OR: 0.61, 95%CI: 0.40-0.94). In Changzhou, we observed a positive correlation between adolescents’ body weight and the number of bus stops in their neighbourhood (OR:1.53, 95%CI:1.06-2.21). ConclusionsThe living environment factors were independently associated with teenagers’ excessive body weight, and the environmental risk factors might be associated with political management. Public policies change students’ living environments, finally affecting individual behaviours and health outcomes.
Genome-Scale Screening of Vaccine Candidates Against Pseudomonas Aeruginosa
Authors: Wan Chuang , Gao Chen , Xie Qin , Wang Yin , Cheng Xin , Fang Yao , Liu Zhiyong , Zhang Weijun , Zou Quanming , Lu Guangwen , Gu Jiang 
Published: October 2020

Abstract
BackgroundInfections due to Pseudomonas aeruginosa (PA) are becoming a serious threat to patients in intensive care units. A PA vaccine is a practical and economical solution to solve the problems caused by PA infection successfully. In recent years, several antigen candidates have been tested in animal and human clinical trials, but none of them has been approved to date. An alternative strategy for antigen screening and protective antigens is in urgent demand.MethodsIn this study, we generated a genome-wide library of PA protein fragments tagged with maltose-binding protein (MBP). Using sera from patients who recovered after PA infection, we identified novel protective antigens and investigate the mechanism of these antigens induced protections.Resultswe identified a novel protective antigen, FlgE, which is the structural component of the flagella hook. Vaccination with recombinant FlgE (reFlgE) induced a Th2-predominant immune response and reduced bacterial load and inflammation in PA-infected mice. Anti-reFlgE antibodies recognized native FlgE on the bacterial membrane in vitro and conferred protection in mice, which may be due to the mediation of opsonophagocytic killing and inhibition of bacterial motility. In addition, the combination of reFlgE with rePcrVNH, an engineered antigen we reported previously, provided elevated protection against PA infection.ConclusionOur data demonstrate that FlgE is a promising vaccine candidate for PA and provide a new strategy for the efficient screening of antigens of other pathogens.
HSP90α Mediates Sorafenib Resistance in Human Hepatocellular Carcinoma by Necroptosis Inhibition Under Hypoxia
Authors: Liao Yan , Yang Yue , Pan Di , Ding Youxiang , Zhang Heng , Ye Yuting , Li Jia , Zhao Li 
Published: October 2020

Abstract
BackgroundAs one of the most common malignancies worldwide, Hepatocellular carcinoma (HCC) has been treated by Sorafenib, which is the first approved target drug by FDA for advanced HCC. However, drug resistance is one of the obstacles to its application. As a typical characteristic of most solid tumors, hypoxia has become a key cause for resistant to chemotherapy and radiotherapy. It is important to elucidate the underlying mechanisms of Sorafenib resistance under hypoxia. 17-AAG, an inhibitor of HSP90α, and is in clinical test at present in anticancer. MethodsRoutine laboratory experimental methods including cell culture, cell transfection, western blot, immunohistochemistry (IHC), and immunofluorescence (IF) were used; the morphological changes of hepatocellular carcinoma cell was observed by Live Cell Imaging System and Transmission Electron Microscope; coimmunoprecipitation (Co-IP) were used for confirmation of interactions of RIPK3/MLKL/HSP90α and HSP70/LAMP2/HSP90α/MLKL. Patient-derived tumor xenograft (PDX) model and human HCC cells xenograft model have been established, and primary resistant cell line was induced to investigate the potential therapeutic strategies to overcome Sorafenib resistance.ResultsIn this study, Sorafenib was found to induce necroptosis in liver cancer. Under hypoxia, the distribution of necroptosis related proteins was changed, which contributed to Sorafenib resistance. HSP90α binds with RIPK1/RIPK3/MLKL complex and promotes chaperone-mediated autophagy (CMA) degradation, which leads necroptosis blocking. Ultimately, the decrease of necroptosis resulted in Sorafenib resistance. 17-AAG inhibited HSP90α and presented obvious reversal effects of Sorafenib resistance in vivo and in vitro. All the results emphasized that the blockage of HSP90α can significantly improve the effect of Sorafenib under hypoxia. ConclusionsHSP90α plays a critical role in Sorafenib resistance under hypoxia by blocking necroptosis. 17-AAG combining with Sorafenib is a promising therapy for hepatocellular carcinoma.
The correlation between vitamin D at birth and growth at 6 months of age in twins: from a prospective longitudinal birth cohort
Authors: Li Xin , Yu Jiaxiao , Wen Li , Tong Chao , Tong Qi , Qi Hongbo , Saffery Richard , Kilby Mark D , Baker Philip N 
Published: October 2020

Abstract
Background: Vitamin D deficiency is a global public health issue in women and children, is associated with adverse impacts on child growth, such as rickets. However, prior studies have mainly focused on measuring vitamin D levels in singleton pregnant women and their offspring, very limited studies have revealed the prevalence of vitamin D deficiency in twin pregnant women and their offspring. The aim of this study was to investigate vitamin D levels in twin pregnant women and their neonates. We also explored the correlation of maternal vitamin D levels with neonatal outcomes and infant growth. Methods: A prospective subcohort investigation was carried out among 72 dichorionic twin pregnant mothers and their twin offspring from the Longitudinal Twin Study. Peripheral blood was collected from the mothers in the third trimester and cord blood was collected form neonates at birth to identify 25[OH]D levels. Data on the characteristics of the mothers and neonates were collected. Infant growth data and food sensitivities were also collected. Results: The average maternal 25[OH]D level was 31.78 ng/mL, with 19.4% being deficiency and 20.8% insufficiency, while the average neonatal 25[OH]D level was 15.37 ng/mL, with 99.3% being deficiency or insufficiency. A positive correlation was found between maternal and neonatal 25[OH]D levels (beta-value: 0.43, 95% CI: 0.37, 0.49). Interestingly, the higher the maternal 25[OH]D level was, the smaller the co-twins birthweight discordance (beta-value: -2.67, 95% CI: -5.11, -0.23). In addition, the infants of mothers with vitamin D deficiency were more likely to be allergic to foods at six months than those of mothers with vitamin D sufficiency. Conclusions: Twin neonates were at extremely high risk of vitamin D deficiency although their mothers’ vitamin D deficiency partially improved. Higher maternal vitamin D level was associated with smaller discordance of co-twins birthweight.Trial registration: ChiCTR-OOC-16008203
Discrimination between spinal cord injury and cervical compressive myelopathy in intramedullary high-intensity lesions on T2-weighted magnetic resonance imaging
Authors: Kamei Naosuke , Nakanishi Kazuyoshi , Nakamae Toshio , Tamaura Takayuki , Tsuchikawa Yuji , Morisako Taiki , Harada Takahiro , Maruyama Toshiaki , Adachi Nobuo 
Published: October 2020

Abstract
Background: When intramedullary high-intensity lesions are found on cervical T2-weighted magnetic resonance images of patients with whiplash injury, it is often required to identify whether they are due to spinal cord injury (SCI) or cervical compressive myelopathy (CCM). This is important to determine insurance coverage. Thus, this study aimed to develop a method to distinguish between SCI and CCM.Methods: Magnetic resonance images of 20 patients who had cervical SCI were evaluated. These 20 SCI patients were age- and sex-matched to 20 CCM patients. The number of discs adjacent to T2 high-intensity lesions was counted because the T2 high-intensity lesions in SCI were characterized by sagittal dilation. The “snake-eyes appearance” was assessed as a characteristic finding of CCM. T2 values in T2 high-intensity lesions were evaluated quantitatively by its ratio with the T2 values in the normal spinal cord, including signal ratio and contrast ratio.Results: The number of discs adjacent to T2 high-intensity lesions was significantly higher in SCI than in CCM. The snake-eyes appearance was found in 9 of 20 CCM patients, but not in SCI patients. The signal ratio and contrast ratio on axial images were significantly higher in SCI than in CCM. A new diagnostic scale was created based on these results. This diagnostic scale has made it possible to distinguish SCI and CCM with >90% accuracy from all perspectives including sensitivity, specificity, positive predictive value, and negative predictive value.Conclusions: The features of T2 high-intensity lesions can be used to distinguish between SCI and CCM.