bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial genetic structure of the invasive tree Robinia pseudoacacia to determine migration patterns to inform best practices for riparian restoration
Authors: Yaegashi Sakiko , Omura Tatsuo , Watanabe Kozo 
Published: August 2020

AbstractThe black locust Robinia pseudoacacia (Robinieae, Fabaceae) is a common invasive riparian tree in Japan. There are less effective management strategies to remove the tree from the riparian area because of its quickly established high population. We investigated the expansion patterns of R. pseudoacacia through sympatric (i.e. between high- and low-water channel (HWC/LWC) within a study site) and allopatric (i.e. along river corridor) dispersal in the Tama River (Tokyo, Japan). Four microsatellites were used to examine the effects of gene flow on six populations in three sites. These subpopulations showed small genetic distance (i.e. no barrier or slightly limited) and genetically mixed population structure. It indicated that both sympatric and allopatric dispersals were active. Many migrants were younger individuals (i.e. &lt;5 years old) and were found in the LWC area. Thus, the LWC could receive more migrants than the HWC through both types of dispersals. In addition, our age and genetic structure analyses reveal that recruited individuals likely settled immediately after the clearing project of R. pseudoacacia through sympatric dispersal. It appears that the migration by allopatric dispersal occurred following this. For the effective management of R. pseudoacacia, migrants should be removed regularly following initial removal of invaders during site restoration.
A lab in the field: applications of real-time, in situ metagenomic sequencing
Authors: Latorre-Pérez Adriel , Pascual Javier , Porcar Manuel , Vilanova Cristina 
Published: August 2020

Abstract
High-throughput metagenomic sequencing is considered one of the main technologies fostering the development of microbial ecology. Widely used second-generation sequencers have enabled the analysis of extremely diverse microbial communities, the discovery of novel gene functions, and the comprehension of the metabolic interconnections established among microbial consortia. However, the high cost of the sequencers and the complexity of library preparation and sequencing protocols still hamper the application of metagenomic sequencing in a vast range of real-life applications. In this context, the emergence of portable, third-generation sequencers is becoming a popular alternative for the rapid analysis of microbial communities in particular scenarios, due to their low cost, simplicity of operation, and rapid yield of results. This review discusses the main applications of real-time, in situ metagenomic sequencing developed to date, highlighting the relevance of this technology in current challenges (such as the management of global pathogen outbreaks) and in the next future of industry and clinical diagnosis.
Acknowledging Post-Traumatic Stress Disorder: Treatment Utilisation Amongst Israeli Bedouin and Jewish Combat Veterans
Authors: Shorer Shai , Caspi Yael , Goldblatt Hadass , Azaiza Faisal 
Published: August 2020

Abstract
Many veterans coping with combat-post-traumatic stress disorder (PTSD) refrain from seeking psychological treatment. We explored the nature of illness recognition and treatment utilisation in two different cultural groups of Israeli veterans—Bedouin and Jewish. Using qualitative research methods, we interviewed twenty veterans dealing with PTSD (ten Bedouin and ten Jewish) and ten mental health professionals. Participants shared their experiences of symptoms, the ways they coped, and their perception of the costs and benefits of psychological treatment. Two main themes emerged: (i) ‘Veterans’ Perceptions of Their Mental Injury’. In stark contrast to their Jewish counterparts, Bedouin participants described extreme lack of understanding regarding the relationship between their exposure to combat trauma and their distress; (ii) ‘Veterans’ Perceptions of the Mental Health Services’. Bedouin veterans expressed fear of the stigma associated with PTSD and its detrimental social implications, for them and their families. Jewish veterans emphasised the beneficial and informative role of therapy. The findings underscore the impact of different cultural codes on help-seeking behaviours and on the ability to benefit from psychological treatment. It is recommended that mental health practitioners consider cultural backgrounds and individual differences when implementing trauma interventions, to fine-tune their suitability to veterans facing excessive barriers to care.
Mixtures of Sweeteners and Maltodextrin Enhance Flavor and Intake of Alcohol in Adolescent Rats
Authors: Sardarian Alice , Liu Sophia , Youngentob Steven L , Glendinning John I 
Published: August 2020

Abstract
Sweet flavorants enhance palatability and intake of alcohol in adolescent humans. We asked whether sweet flavorants have similar effects in adolescent rats. The inherent flavor of ethanol in adolescent rats is thought to consist of an aversive odor, bitter/sweet taste, and burning sensation. In Experiment 1, we compared ingestive responses of adolescent rats to 10% ethanol solutions with or without added flavorants using brief-access lick tests. We used 4 flavorants, which contained mixtures of saccharin and sucrose or saccharin, sucrose, and maltodextrin. The rats approached (and initiated licking from) the flavored ethanol solutions more quickly than they did unflavored ethanol, indicating that the flavorants attenuated the aversive odor of ethanol. The rats also licked at higher rates for the flavored than unflavored ethanol solutions, indicating that the flavorants increased the naso-oral acceptability of ethanol. In Experiment 2, we offered rats chow, water, and a flavored or unflavored ethanol solution every other day for 8 days. The rats consistently consumed substantially more of the flavored ethanol solutions than unflavored ethanol across the 8 days. When we switched the rats from the flavored to unflavored ethanol for 3 days, daily intake of ethanol plummeted. We conclude that sweet and sweet/maltodextrin flavorants promote high daily intake of ethanol in adolescent rats (i.e., 6–10 g/kg) and that they do so in large part by improving the naso-oral sensory attributes of ethanol.
Inoculation Age of Bacillus Calmette-Guérin Tokyo-172 Strain and Vaccine-related Adverse Reactions in Taiwan Birth Cohort of 2012–2017
Authors: Huang Wei , Chiu Nan-Chang , Chi Hsin , Huang Fu-Yuan , Huang Ching-Ying 
Published: August 2020

Abstract

Background
In 2016 in Taiwan, inoculation with the Bacillus Calmette-Guérin (BCG) Tokyo-172 vaccine was postponed from 24 hours after birth to 5–8 months of age. We reviewed BCG-induced adverse reactions reported to the Vaccine Injury Compensation Program (VICP) to identify differences between early and delayed BCG inoculation.

Methods
Clinical presentations of BCG-related adverse reactions reported to VICP for the 2012–2017 birth cohort were reviewed until the end of 2019. The correlations between inoculation age and complications were evaluated.

Results
We analyzed 233 BCG adverse reactions, including regional lymphadenitis (33.9%), injection site reactions (35.2%), osteitis/osteomyelitis (27.9%), and distant soft tissue infections (3.0%). The incidence of osteitis/osteomyelitis was lower when vaccination was done after 5 months of age (relative risk [RR], 0.32; 95% confidence interval [CI], .16–.64). Injection site reactions (RR, 8.82; 95% CI, 5.04–15.44) and lymphadenitis (RR, 2.24; 95% CI, 1.44–3.45) were significantly more common in vaccinees older than 5 months. Shorter onset durations of mild adverse reactions (lymphadenitis and injection site reactions) were reported in vaccinees older than 5 months, while no statistical significance was found regarding osteitis/osteomyelitis.

Conclusions
Osteomyelitis and distant soft tissue infection may occur less frequently when BCG inoculation occurs after 5 months of age, although mild adverse reactions can be more frequent, symptom onset times can be shortened. As few severe reactions might occur more than 2 years after BCG inoculation and the policy of delayed BCG inoculation was implemented in 2016, a longer observational period is needed to clarify the exact severe complications decrement.
Top management gender diversity and performance: in search of threshold effects
Authors: Gong Yundan , Girma Sourafel 
Published: August 2020

Abstract
The impact of gender diversity on business performance has been featured prominently on the agenda of many politicians and business leaders in recent years. However, empirical results of the impact of gender diversity on firm performance have been ambiguous. This paper contributes to the literature by using propensity score-based estimation techniques on a large sample of UK firms to analyse the performance effect of appointing a first female board director. We look at financial and non-financial performance indicators and document significant effects on firm growth and labour cost efficiency, but rather fragile ones on accounting returns, such as profitability. We also document evidence of another threshold effect; namely, gender diversity appears to have its highest impact (its ‘ceiling’) when the proportion of female directors is approximately 30%. Carrying out a sensitivity analysis, we conclude that hidden bias must be implausibly high to be able to attribute the beneficial effects of boardroom gender diversity to unmeasured confounding.
Right versus left heart reverse remodelling after treating ischaemic mitral and tricuspid regurgitation
Authors: Elgharably Haytham , Javadikasgari Hoda , Koprivanac Marijan , Lowry Ashley M , Sato Kimi , Blackstone Eugene H , Klein Allan L , Gillinov A Marc , Svensson Lars G , Navia José L 
Published: August 2020

AbstractOBJECTIVESRepair outcomes of tricuspid regurgitation (TR) associated with ischaemic mitral regurgitation (IMR) are inferior to functional TR in terms of TR recurrence and right ventricular (RV) reverse remodelling. Our objective is to analyse right versus left heart reverse remodelling after surgery for IMR-associated TR.METHODSFrom 2001 to 2011, 568 patients with severe IMR underwent mitral valve surgery (repair 87%, replacement 13%), and 131 had concomitant tricuspid valve repair. Median follow-up was 3.0 years; 25% of living patients were followed up for 6.3 years. Longitudinal analysis of 1527 follow-up echocardiograms was performed to assess ventricular reverse remodelling and function.RESULTSUnlike the left heart, the right heart failed to reverse remodel (failed to recover ventricular function or halt dilatation). During follow-up after surgery, the right ventricle continued to dilate while the left ventricle regressed in size. RV ejection fraction decreased (46% at 1 month and 44% at 5 years), while left ventricular ejection fraction increased (33% and 37%, respectively). RV strain showed early (−11% at 1 month) and late (−12% at 5 years) dysfunction. Patients who underwent tricuspid valve repair had worse RV function. Mitral regurgitation remained stable after surgical intervention, and TR gradually recurred (37% moderate, 20% severe at 7 years).CONCLUSIONSSurgical treatment of IMR and TR along with revascularization failed to induce reverse remodelling of the right heart. These findings warrant further investigations to identify optimal timing and approach of intervention for IMR-associated TR with respect to RV remodelling.
National trends and perioperative outcomes of robotic oesophagectomy following induction chemoradiation therapy: a National Cancer Database propensity-matched analysis
Authors: Kamel Mohamed K , Sholi Adam N , Rahouma Mohamed , Harrison Sebron W , Lee Benjamin , Stiles Brendon M , Altorki Nasser K , Port Jeffrey L 
Published: August 2020

Abstract

OBJECTIVES
Oesophagectomy following induction chemoradiation therapy (CRT) is technically challenging. To date, little data exist to describe the feasibility of a robotic approach in this setting. In this study, we assessed national trends and outcomes of robotic oesophagectomy following induction CRT compared to the traditional open approach.

METHODS
The National Cancer Database was queried for patients who underwent oesophagectomy following induction CRT (2010–2014). Trends of robotic utilization were assessed by a Mantel–Haenszel test of trend. Propensity matching controlled for differences in age, gender, comorbidity, stage, histology and tumour location between the robotic and open groups. Overall survival was estimated by Kaplan–Meier analysis and compared by a log-rank test

RESULTS
Oesophagectomy following induction CRT was performed in 6958 patients. Of them, 555 patients (8%) underwent robotic surgery (5% converted to an open approach). Between 2010 and 2014, utilization of a robotic approach increased from 3% to 11% (Mantel–Haenszel, P &lt; 0.001) and the number of hospitals performing at least 1 robotic oesophagectomy increased from 23 to 57. Compared to the traditional open approach, robotic oesophagectomy was used more frequently at academic hospitals (76% vs 60%, P &lt; 0.001), and in patients living in metropolitan areas (85% vs 77%, P &lt; 0.001) and those living in the Midwest (41% vs 33%, P &lt; 0.001). In the matched groups, a robotic approach was associated with shorter median hospital stay (9 vs 10 days, P = 0.004) and dissection of more lymph nodes (median, 16 vs 12, P &lt; 0.001). However, there were no differences in rates of positive margin resection (5% for both groups, P = 0.95), 30-day readmissions (5% vs 7%, P = 0.18), 30-day mortality (2.5% vs 4%, P = 0.79), 90-day mortality (9% vs 8.5%, P = 0.89) or 5-year overall survival (42% vs 39%, P = 0.19) between patients undergoing robotic and open surgery, respectively.

CONCLUSIONS
Robotic oesophagectomy after induction CRT is feasible and associated with shorter hospitalization compared to an open approach, and does not compromise the adequacy of oncological resection, perioperative outcomes or long-term survival.
Draft Genomes of Amaranthus tuberculatus, Amaranthus hybridus, and Amaranthus palmeri
Authors: Montgomery Jacob S , Giacomini Darci , Waithaka Bridgit , Lanz Christa , Murphy Brent P , Campe Ruth , Lerchl Jens , Landes Andreas , Gatzmann Fanny , Janssen Antoine , Antonise Rudie , Patterson Eric , Weigel Detlef , Tranel Patrick J 
Published: August 2020

Abstract
Amaranthus tuberculatus, Amaranthus hybridus, and Amaranthus palmeri are agronomically important weed species. Here, we present the most contiguous draft assemblies of these three species to date. We utilized a combination of Pacific Biosciences long-read sequencing and chromatin contact mapping information to assemble and order sequences of A. palmeri to near-chromosome-level resolution, with scaffold N50 of 20.1 Mb. To resolve the issues of heterozygosity and coassembly of alleles in diploid species, we adapted the trio binning approach to produce haplotype assemblies of A. tuberculatus and A. hybridus. This approach resulted in an improved assembly of A. tuberculatus, and the first genome assembly for A. hybridus, with contig N50s of 2.58 and 2.26 Mb, respectively. Species-specific transcriptomes and information from related species were used to predict transcripts within each assembly. Syntenic comparisons of these species and Amaranthus hypochondriacus identified sites of genomic rearrangement, including duplication and translocation, whereas genetic map construction within A. tuberculatus highlighted the need for further ordering of the A. hybridus and A. tuberculatus contigs. These multiple reference genomes will accelerate genomic studies in these species to further our understanding of weedy evolution within Amaranthus.
Older Latino Mental Health: A Complicated Picture
Authors: Jimenez Daniel E , Martinez Garza David , Cárdenas Verónica , Marquine María 
Published: August 2020

Abstract
The aggregation of Latino subgroups in national studies creates an overly simplistic narrative that Latinos are at lower risk of mental illness and that foreign nativity seems protective against mental illness (i.e., immigrant paradox). This broad generalization does not hold up as the Latino population ages. Given that social inequalities for risk appear to widen with age, the social disadvantages of being Latino in the United States increase the risk for mental illness across the life span. This review focuses on the mental health of older Latinos, specifically the 3 subgroups with the longest residential history in the United States—Mexicans, Puerto Ricans, and Cubans. We examine relevant epidemiological and clinical psychopathology studies on aging in these Latino populations and present evidence of the heterogeneity of the older Latino population living in the United States, thus illustrating a limitation in this field—combining Latino subgroups despite their diversity because of small sample sizes. We address the migration experience—how intraethnic differences and age of migration affect mental health—and discuss social support and discrimination as key risk and protective factors. We conclude with a discussion on meeting the mental health needs of older Latinos with a focus on prevention, a promising approach to addressing mental illness in older Latinos, and future directions for mental health research in this population. Success in this endeavor would yield a substantial reduction in the burden of late-life depression and anxiety and a positive public health impact.
A haplotype-resolved, de novo genome assembly for the wood tiger moth (Arctia plantaginis) through trio binning
Authors: Yen Eugenie C , McCarthy Shane A , Galarza Juan A , Generalovic Tomas N , Pelan Sarah , Nguyen Petr , Meier Joana I , Warren Ian A , Mappes Johanna , Durbin Richard , Jiggins Chris D 
Published: August 2020

ABSTRACT

Background
Diploid genome assembly is typically impeded by heterozygosity because it introduces errors when haplotypes are collapsed into a consensus sequence. Trio binning offers an innovative solution that exploits heterozygosity for assembly. Short, parental reads are used to assign parental origin to long reads from their F1 offspring before assembly, enabling complete haplotype resolution. Trio binning could therefore provide an effective strategy for assembling highly heterozygous genomes, which are traditionally problematic, such as insect genomes. This includes the wood tiger moth (Arctia plantaginis), which is an evolutionary study system for warning colour polymorphism.

Findings
We produced a high-quality, haplotype-resolved assembly for Arctia plantaginis through trio binning. We sequenced a same-species family (F1 heterozygosity ~1.9%) and used parental Illumina reads to bin 99.98% of offspring Pacific Biosciences reads by parental origin, before assembling each haplotype separately and scaffolding with 10X linked reads. Both assemblies are contiguous (mean scaffold N50: 8.2 Mb) and complete (mean BUSCO completeness: 97.3%), with annotations and 31 chromosomes identified through karyotyping. We used the assembly to analyse genome-wide population structure and relationships between 40 wild resequenced individuals from 5 populations across Europe, revealing the Georgian population as the most genetically differentiated with the lowest genetic diversity.

Conclusions
We present the first invertebrate genome to be assembled via trio binning. This assembly is one of the highest quality genomes available for Lepidoptera, supporting trio binning as a potent strategy for assembling heterozygous genomes. Using our assembly, we provide genomic insights into the geographic population structure of A. plantaginis.
Humidity related magnetite alteration in an experimental setup
Authors: Zhang Qi , Appel Erwin , Stanjek Helge , Byrne James M , Berthold Christoph , Sorwat Julian , Rösler Wolfgang , Seemann Timo 
Published: August 2020

SUMMARY
Low-temperature oxidation (LTO) of magnetite is an alteration process which occurs under normal atmospheric conditions, causing maghemitization. The use of magnetic properties as palaeoclimate proxies requires improved understanding of how humidity and temperature affect such processes. We exposed natural magnetite, with grain size ranging from &lt;1 to ∼30 μm, to different humidity conditions at room temperature and 70 °C for 1 yr. Changes in room temperature setups were very minor, but in all 70 °C setups alteration was detected by magnetic and mineralogical properties. Lowering of the Verwey transition temperature (Tv) turned out to be the most sensitive indicator of LTO, and also lattice constants correlate well with the shift of Tv. Thermomagnetic curves and XRD-results indicate that LTO affects the entire volume of the particles rather than only surface layers. The sample exposed to high relative humidity (rH) &gt;90 per cent at 70 °C showed the strongest degree of LTO with an increase of the oxidation degree by ∼3 per cent according to Tv, and it was the only setup where partial alteration to hematite was indicated by Mössbauer analysis. The sample with extremely dry conditions (rH of ∼5 per cent) at 70 °C, and the sample that was exposed to cycles of high and low humidity in 2-weeks alternation at 70 °C, both revealed a smaller degree of LTO. The smallest change of the high temperature setups was observed for the sample with intermediate rH of ∼13 per cent. The results suggest a non-linear sensitivity of magnetite alteration to humidity conditions, high humidity strongly favours alteration, but alteration is strongly reduced when extreme humidity alternates with dry conditions, suggesting an importance of seasonality in natural weathering.
The Gut Microbiome as a Reservoir for Antimicrobial Resistance
Authors: Anthony Winston E , Burnham Carey-Ann D , Dantas Gautam , Kwon Jennie H 
Published: August 2020

Abstract
This review will consider the gut as a reservoir for antimicrobial resistance, colonization resistance, and how disruption of the microbiome can lead to colonization by pathogenic organisms. There is a focus on the gut as a reservoir for β-lactam and plasmid-mediated quinolone resistance. Finally, the role of functional metagenomics and long-read sequencing technologies to detect and understand antimicrobial resistance genes within the gut microbiome is discussed, along with the potential for future microbiome-directed methods to detect and prevent infection.
Circulating Endothelial Cells as a Marker of Endothelial Injury in Severe COVID -19
Authors: Guervilly Christophe , Burtey Stephane , Sabatier Florence , Cauchois Raphaël , Lano Guillaume , Abdili Evelyne , Daviet Florence , Arnaud Laurent , Brunet Philippe , Hraiech Sami , Jourde-Chiche Noémie , Koubi Marie , Lacroix Romaric , Pietri Léa , Berda Yaël , Robert Thomas , Degioanni Clara , Velier Mélanie , Papazian Laurent , Kaplanski Gilles , Dignat-George Françoise 
Published: August 2020

Abstract
Beside the commonly described pulmonary expression of the coronavirus disease 2019 (COVID-19), major vascular events have been reported. The objective of this study was to investigate whether increased levels of circulating endothelial cells (CECs) might be associated with severe forms of COVID-19. Ninety-nine patients with COVID-19 were enrolled in this retrospective study. Patients in the intensive care units (ICU) had significantly higher CEC counts than non-ICU patients and the extent of endothelial injury was correlated with putative markers of disease severity and inflammatory cytokines. Together, these data provide in vivo evidence that endothelial injury is a key feature of COVID-19.