bluuebunny/crossref_metadata_2025_split huggingface dataset:
High degree of consensus achieved regarding diagnosis and treatment of acromioclavicular joint instability among ESA-ESSKA members
Authors: Rosso Claudio , Martetschläger Frank , Saccomanno Maristella F. , Voss Andreas , Lacheta Lucca ,   ESA DELPHI Consensus Panel, Ângelo Ana Catarina , Antonogiannakis Emmanuel , Azevedo Clara , Bak Klaus , Becirbegovic Semin , Beitzel Knut , Bilsel Kerem , Brzoska Roman , Calvo Angel , Charousset Christophe , Dyrna Felix , Brilakis Emmanuel , Franceschi Francesco , Glasson Jean Marc , Gleyze Pascal , Gomes Nuno , Hackney Roger , Hantes Michael , Karargyris Orestis , Karahan Mustafa , Kovacic Ladislav , Kubashev Alexander , Lacheta Lucca , Lorbach Olaf , Maben Benjo , Marjanovic Benjamin , Martetschlaeger Frank , Yiannakopolus Christos , Ostermann Roman C , Panagopoulos Andreas , Papadopoulos Perikles , Poberaj Boris , Rosso Claudio , Saccomanno Maristella Francesca , Smolen Daniel , Soler Francesc , Taverna Ettore , Toussaint Bruno , Vavken Patrick , Voss Andreas , Zurita Nestor , Beitzel Knut , Milano Giuseppe 
Published: September 2020

Abstract
Purpose
To develop a consensus on diagnosis and treatment of acromioclavicular joint instability.

Methods
A consensus process following the modified Delphi technique was conducted. Panel members were selected among the European Shoulder Associates of ESSKA. Five rounds were performed between October 2018 and November 2019. The first round consisted of gathering questions which were then divided into blocks referring to imaging, classifications, surgical approach for acute and chronic cases, conservative treatment. Subsequent rounds consisted of condensation by means of an online questionnaire. Consensus was achieved when ≥ 66.7% of the participants agreed on one answer. Descriptive statistic was used to summarize the data.

Results
A consensus was reached on the following topics. Imaging: a true anteroposterior or a bilateral Zanca view are sufficient for diagnosis. 93% of the panel agreed on clinical override testing during body cross test to identify horizontal instability. The Rockwood classification, as modified by the ISAKOS statement, was deemed valid. The separation line between acute and chronic cases was set at 3 weeks. The panel agreed on arthroscopically assisted anatomic reconstruction using a suspensory device (86.2%), with no need of a biological augmentation (82.8%) in acute injuries, whereas biological reconstruction of coracoclavicular and acromioclavicular ligaments with tendon graft was suggested in chronic cases. Conservative approach and postoperative care were found similar

Conclusion
A consensus was found on the main topics of controversy in the management of acromioclavicular joint dislocation. Each step of the diagnostic treatment algorithm was fully investigated and clarified.

Level of evidence
Level V.
Finite-dimensional pointed Hopf algebras over finite simple groups of Lie type V. Mixed classes in Chevalley and Steinberg groups
Authors: Andruskiewitsch Nicolás , Carnovale Giovanna , García Gastón Andrés 
Published: September 2020

AbstractWe show that all classes that are neither semisimple nor unipotent in finite simple Chevalley or Steinberg groups different from$$\mathbf {PSL}_n(q)$$PSLn(q)collapse (i.e. are never the support of a finite-dimensional Nichols algebra). As a consequence, we prove that the only finite-dimensional pointed Hopf algebra whose group of group-like elements is$$\mathbf {PSp}_{2n}(q)$$PSp2n(q),$$\mathbf {P}{\varvec{\Omega }}^+_{4n}(q)$$PΩ4n+(q),$$\mathbf {P}{\varvec{\Omega }}^-_{4n}(q)$$PΩ4n-(q),$$^3D_4(q)$$3D4(q),$$E_7(q)$$E7(q),$$E_8(q)$$E8(q),$$F_4(q)$$F4(q), or$$G_2(q)$$G2(q)withqeven is the group algebra.
Evaluation of a new adipose tissue processing method for breast and buttock fat grafting procedures
Authors: Auclair Eric , Gianfermi Massimo 
Published: September 2020

Abstract
Background
There is still some debate about the best adipose processing method for autologous fat transfer procedures. This article reports a series of 66 fat grafting procedures using a novel fat processing technique that involves washing, “micronization,” and decantation.

Methods
We retrospectively reviewed the files of all patients operated on over a 2-year period by the two investigators. They underwent breast or buttock surgery that employed the novel fat processing technique for fat transfer. Complications and patient satisfaction were recorded, and fat resorption was qualitatively assessed based on clinical examination and photographs.

Results
Data were retrieved from a total of 66 procedures performed on 43 patients: 17 breast augmentations, 8 buttock augmentations, and 41 breast reconstructions. Five patients also underwent concomitant facial fat grafting. The complications were one case of transient liponecrotic cyst in the breast, one case of transient areolar sensitivity reduction, and two cases of infection. The infections were reported in patients from the breast reconstruction cohort who had a history of radiotherapy. There was no complication associated with facial fat grafting. Patient satisfaction was achieved in 82.4 to 92.5% of cases depending on the study cohort. Fat resorption was graded as minimal to moderate in 95% of all cases.

Conclusions
This retrospective study supports the safety and effectiveness of this novel fat processing method in breast and buttock surgeries. Further controlled studies are needed to compare its performance with that of other techniques.
Level of evidence: Level III, therapeutic study
A Case Study of Eukaryogenesis: The Evolution of Photoreception by Photolyase/Cryptochrome Proteins
Authors: Miles Jennifer A. , Davies Thomas A. , Hayman Robert D. , Lorenzen Georgia , Taylor Jamie , Anjarwalla Mubeena , Allen Sammie J. R. , Graham John W. D. , Taylor Paul C. 
Published: September 2020

AbstractEukaryogenesis, the origin of the eukaryotes, is still poorly understood. Herein, we show how a detailed all-kingdom phylogenetic analysis overlaid with a map of key biochemical features can provide valuable clues. The photolyase/cryptochrome family of proteins are well known to repair DNA in response to potentially harmful effects of sunlight and to entrain circadian rhythms. Phylogenetic analysis of photolyase/cryptochrome protein sequences from a wide range of prokaryotes and eukaryotes points to a number of horizontal gene transfer events between ancestral bacteria and ancestral eukaryotes. Previous experimental research has characterised patterns of tryptophan residues in these proteins that are important for photoreception, specifically a tryptophan dyad, a canonical tryptophan triad, an alternative tryptophan triad, a tryptophan tetrad and an alternative tetrad. Our results suggest that the spread of the different triad and tetrad motifs across the kingdoms of life accompanied the putative horizontal gene transfers and is consistent with multiple bacterial contributions to eukaryogenesis.
Detection of EP300-ZNF384 fusion in patients with acute lymphoblastic leukemia using RNA fusion gene panel sequencing
Authors: Jing Yu , Li Yan-Fen , Wan Hua , Liu Dai-Hong 
Published: September 2020

AbstractEP300-ZNF384 fusion is a rare recurrent cytogenetic abnormality associated with B cell acute lymphoblastic leukemia (B-ALL), which was rarely studied in Chinese patient cohort. Here, we used a customized RNA fusion gene panel to investigate gene fusions in 56 selected acute leukemia patients without conventional genetic abnormalities. Two EP300-ZNF384 fusion forms were detected in ten cases, which were in-frame fusions of EP300 exon 6 fused with exon 3 or 2 of ZNF384. The fusions led to the lack of most functional domains of EP300. We firstly reported EP300-ZNF384 fusion in a mixed-phenotype acute leukemia (MPAL) patient whose CD33 and CD13 were negative. The rest nine B-ALL patients with EP300-ZNF384 fusion expressed CD33 and/or CD13. Fifty-six percent of B-ALL patients (5/9) with EP300-ZNF384 fusion were positive with CD10. After the diagnosis of EP300-ZNF384 fusion, 70% of the patients achieved remission after chemotherapy. Our observations indicated that EP300-ZNF384 fusion consists of a distinct subgroup of B-ALL with a characteristic immunophenotype. These patients are sensitive to current chemotherapy regimen and have an excellent outcome.
Functional analysis of Cti6 core domain responsible for recruitment of epigenetic regulators Sin3, Cyc8 and Tup1
Authors: Aref Rasha , Schüller Hans-Joachim 
Published: September 2020

AbstractMapping of effective protein domains is a demanding stride to disclose the functional relationship between regulatory complexes. Domain analysis of protein interactions is requisite for understanding the pleiotropic responses of the respective partners. Cti6 is a multifunctional regulator for which we could show recruitment of co-repressors Sin3, Cyc8 and Tup1. However, the responsible core domain tethering Cti6 to these co-repressors is poorly understood. Here, we report the pivotal domain of Cti6 that is indispensable for co-repressor recruitment. We substantiated that amino acids 450–506 of Cti6 bind PAH2 of Sin3. To analyse this Cti6–Sin3 Interaction Domain (CSID) in more detail, selected amino acids within CSID were replaced by alanine. It is revealed that hydrophobic amino acids V467, L481 and L491 L492 L493 are important for Cti6–Sin3 binding. In addition to PAH2 of Sin3, CSID also binds to tetratricopeptide repeats (TPR) of Cyc8. Indeed, we could demonstrate Cti6 recruitment to promoters of genes, such as RNR3 and SMF3, containing iron-responsive elements (IRE). Importantly, Sin3 is also recruited to these promoters but only in the presence of functional Cti6. Our findings provide novel insights toward the critical interaction domain in the co-regulator Cti6, which is a component of regulatory complexes that are closely related to chromatin architecture and the epigenetic status of genes that are regulated by pleiotropic co-repressors.
Cardiovascular risk profiles of GnRH agonists and antagonists: real-world analysis from UK general practice
Authors: Davey Patrick , Kirby Mike G. 
Published: September 2020

Abstract
Purpose
Androgen deprivation therapy (ADT) is the mainstay for the management of metastatic prostate cancer. Available pharmaceutical ADTs include gonadotropin-releasing hormone (GnRH) agonists and antagonists. Here, real-world data are presented from the UK general practitioner Optimum Patient Care Research Database. The study investigated the hypothesis that GnRH antagonists have lower cardiac event rates than GnRH agonists.

Methods
The incidence of cardiac events following initiation of GnRH antagonist or agonist therapy was investigated in a population-based cohort study conducted in UK primary care between 2010 and 2017.

Results
Analysis of real-world data from the UK primary care setting showed that relative risk of experiencing cardiac events was significantly lower with degarelix, a GnRH antagonist, compared with GnRH agonists (risk ratio: 0.39 [95% confidence interval 0.191, 0.799]; p = 0.01). Patients that received degarelix as first-line treatment switched treatment more frequently (33.7%), often to a GnRH agonist, than those who initiated treatment with a GnRH agonist (6.7–18.6%).

Conclusion
Screening for known or underlying vascular disease and identifying those at high risk of a cardiac event is important for risk mitigation in patients with prostate cancer receiving hormone therapy. The GnRH antagonist degarelix conferred a significantly lower risk of cardiac events than GnRH agonists. Prior to treatment, patients should be stratified based on level of cardiovascular (CV) risk, and appropriate lifestyle, and pharmacological interventions to mitigate CV risk should be recommended. CV risk factors and patient response to the intervention should be monitored at regular intervals.
Effects of acute warming on cardiac and myotomal sarco(endo)plasmic reticulum ATPase (SERCA) of thermally acclimated brown trout (Salmo trutta)
Authors: Vornanen Matti 
Published: September 2020

AbstractAt high temperatures, ventricular beating rate collapses and depresses cardiac output in fish. The role of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) in thermal tolerance of ventricular function was examined in brown trout (Salmo trutta) by measuring heart SERCA and comparing it to that of the dorsolateral myotomal muscle. Activity of SERCA was measured from crude homogenates of cold-acclimated (+ 3 °C, c.a.) and warm-acclimated (+ 13 °C, w.a.) brown trout as cyclopiazonic acid (20 µM) sensitive Ca2+-ATPase between + 3 and + 33 °C. Activity of the heart SERCA was significantly higher in c.a. than w.a. trout and increased strongly between + 3 and + 23 °C with linear Arrhenius plots but started to plateau between + 23 and + 33 °C in both acclimation groups. The rate of thermal inactivation of the heart SERCA at + 35 °C was similar in c.a. and w.a. fish. Activity of the muscle SERCA was less temperature dependent and more heat resistant than that of the heart SERCA and showed linear Arrhenius plots between + 3 and + 33 °C in both c.a. and w.a. fish. SERCA activity of the c.a. muscle was slightly higher than that of w.a. muscle. The rate of thermal inactivation at + 40 °C was similar for both c.a. and w.a. muscle SERCA at + 40 °C. Although the heart SERCA is more sensitive to high temperatures than the muscle SERCA, it is unlikely to be a limiting factor for heart rate, because its heat tolerance, unlike that of the ventricular beating rate, was not changed by temperature acclimation.
Kilometer-scale modeling projects a tripling of Alaskan convective storms in future climate
Authors: Poujol Basile , Prein Andreas F. , Newman Andrew J. 
Published: September 2020

AbstractConvective storms produce heavier downpours and become more intense with climate change. Such changes could be even amplified in high-latitudes since the Arctic is warming faster than any other region in the world and subsequently moistening. However, little attention has been paid to the impact of global warming on intense thunderstorms in high latitude continental regions, where they can produce flash flooding or ignite wildfires. We use a model with kilometer-scale grid spacing to simulate Alaska’s climate under present and end of the century high emission scenario conditions. The current climate simulation is able to capture the frequency and intensity of hourly precipitation compared to rain gauge data. We apply a precipitation tracking algorithm to identify intense, organized convective systems, which are projected to triple in frequency and extend to the northernmost regions of Alaska under future climate conditions. Peak rainfall rates in the core of the storms will intensify by 37% in line with atmospheric moisture increases. These results could have severe impacts on Alaska’s economy and ecology since floods are already the costliest natural disaster in central Alaska and an increasing number of thunderstorms could result in more wildfires ignitions.
Evaluating the efficacy of treatment options for anal intraepithelial neoplasia: a systematic review
Authors: Brogden Danielle R. L. , Walsh Una , Pellino Gianluca , Kontovounisios Christos , Tekkis Paris , Mills Sarah C. 
Published: September 2020

Abstract
Purpose
Anal intraepithelial neoplasia (AIN) is the accepted precursor of anal squamous cell carcinoma (ASCC). There has long been a hypothesis that treating AIN may prevent ASCC. Many different treatment modalities have been suggested and studied. We conducted this systematic review to evaluate their efficacy and the evidence as to whether we can prevent ASCC by treating AIN.

Methods
MEDLINE and EMBASE were electronically searched using relevant search terms. All studies investigating the use of a single treatment for AIN that reported at least one end outcome such as partial or complete response to treatment, recurrence after treatment and/or ASCC diagnosis after treatment were included.

Results
Thirty studies were included in the systematic review investigating 10 treatment modalities: 5% imiquimod, 5-fluorouracil, cidofovir, trichloroacetic acid, electrocautery, surgical excision, infrared coagulation, radiofrequency ablation, photodynamic therapy and HPV vaccination. All treatment modalities demonstrated some initial regression of AIN after treatment; however, recurrence rates were high especially in HIV-positive patients. Many of the studies suffered from significant bias which prevented direct comparison.

Conclusions
Although the theory persists that by inducing the regression of AIN, we may be able to reduce the risk of ASCC, there was no clinical evidence within the literature advocating that treating AIN does prevent ASCC.
Mosaic trisomy of chromosome 1q in human brain tissue associates with unilateral polymicrogyria, very early-onset focal epilepsy, and severe developmental delay
Authors: Kobow Katja , Jabari Samir , Pieper Tom , Kudernatsch Manfred , Polster Tilman , Woermann Friedrich G. , Kalbhenn Thilo , Hamer Hajo , Rössler Karl , Mühlebner Angelika , Spliet Wim G. M. , Feucht Martha , Hou Yanghao , Stichel Damian , Korshunov Andrey , Sahm Felix , Coras Roland , Blümcke Ingmar , von Deimling Andreas 
Published: September 2020

AbstractPolymicrogyria (PMG) is a developmental cortical malformation characterized by an excess of small and frustrane gyration and abnormal cortical lamination. PMG frequently associates with seizures. The molecular pathomechanisms underlying PMG development are not yet understood. About 40 genes have been associated with PMG, and small copy number variations have also been described in selected patients. We recently provided evidence that epilepsy-associated structural brain lesions can be classified based on genomic DNA methylation patterns. Here, we analyzed 26 PMG patients employing array-based DNA methylation profiling on formalin-fixed paraffin-embedded material. A series of 62 well-characterized non-PMG cortical malformations (focal cortical dysplasia type 2a/b and hemimegalencephaly), temporal lobe epilepsy, and non-epilepsy autopsy controls was used as reference cohort. Unsupervised dimensionality reduction and hierarchical cluster analysis of DNA methylation profiles showed that PMG formed a distinct DNA methylation class. Copy number profiling from DNA methylation data identified a uniform duplication spanning the entire long arm of chromosome 1 in 7 out of 26 PMG patients, which was verified by additional fluorescence in situ hybridization analysis. In respective cases, about 50% of nuclei in the center of the PMG lesion were 1q triploid. No chromosomal imbalance was seen in adjacent, architecturally normal-appearing tissue indicating mosaicism. Clinically, PMG 1q patients presented with a unilateral frontal or hemispheric PMG without hemimegalencephaly, a severe form of intractable epilepsy with seizure onset in the first months of life, and severe developmental delay. Our results show that PMG can be classified among other structural brain lesions according to their DNA methylation profile. One subset of PMG with distinct clinical features exhibits a duplication of chromosomal arm 1q.
Postoperative pain, pain management, and recovery at home after pediatric tonsil surgery
Authors: Alm Fredrik , Lundeberg Stefan , Ericsson Elisabeth 
Published: September 2020

Abstract
Purpose
To explore the severity and duration of postoperative pain, the management of analgesics, and postoperative recovery in children undergoing tonsil surgery.

Method
Participants included 299 children aged 4–17 years undergoing tonsillotomy ± adenoidectomy (TT ± A) or tonsillectomy ± adenoidectomy (TE ± A). Data were collected up to 12 days. The child rated pain on the Face Pain Scale-Revised (FPS-R) and recovery using the Postoperative Recovery in Children (PRiC) questionnaire. Caregivers assessed their child's pain, anxiety, and nausea on a numeric analog scale and kept a log of analgesic administration.

Results
High pain levels (FPS-R ≥ 4) were reported in all surgical and age groups (TT ± A age 4–11, TE ± A age 4–11, TE ± A age 12–17), but there were variations in pain intensity and duration within and between groups. The TE ± A group scored more days with moderate to very excruciating pain and lower recovery than the TT ± A group, with the worst outcomes reported by older TE ± A children. The majority of the children used paracetamol + COX-inhibitors at home, but regular administration of analgesics was lacking, particularly during late evening and at night. Few were received rescue medication (opioid or clonidine) despite severe pain. Physical symptoms and daily life activities were affected during the recovery period. There was moderate agreement between child and the caregiver’s pain assessment scores.

Conclusion
Children reported a troublesome recovery with significant postoperative pain, particularly older children undergoing tonsillectomy. Pain treatment at home was suboptimal and lacked regular analgesic administration. Patient information needs to be improved regarding the importance of regular administration of analgesics and rescue medication.
A NOTCH3 homozygous nonsense mutation in familial Sneddon syndrome with pediatric stroke
Authors: Greisenegger Elli Katharine , Llufriu Sara , Chamorro Angel , Cervera Alvaro , Jimenez-Escrig Adriano , Rappersberger Klemens , Marik Wolfgang , Greisenegger Stefan , Stögmann Elisabeth , Kopp Tamara , Strom Tim M. , Henes Jörg , Joutel Anne , Zimprich Alexander 
Published: September 2020

AbstractSneddon syndrome is a rare disorder affecting small and medium-sized blood vessels that is characterized by the association of livedo reticularis and stroke. We performed whole-exome sequencing (WES) in 2 affected siblings of a consanguineous family with childhood-onset stroke and identified a homozygous nonsense mutation within the epidermal growth factor repeat (EGFr) 19 of NOTCH3, p.(Arg735Ter). WES of 6 additional cases with adult-onset stroke revealed 2 patients carrying heterozygous loss-of-function variants in putative NOTCH3 downstream genes, ANGPTL4, and PALLD. Our findings suggest that impaired NOTCH3 signaling is one underlying disease mechanism and that bi-allelic loss-of-function mutation in NOTCH3 is a cause of familial Sneddon syndrome with pediatric stroke.
Artificial intelligence to detect MYC translocation in slides of diffuse large B-cell lymphoma
Authors: Swiderska-Chadaj Zaneta , Hebeda Konnie M. , van den Brand Michiel , Litjens Geert 
Published: September 2020

AbstractIn patients with suspected lymphoma, the tissue biopsy provides lymphoma confirmation, classification, and prognostic factors, including genetic changes. We developed a deep learning algorithm to detect MYC rearrangement in scanned histological slides of diffuse large B-cell lymphoma. The H&E-stained slides of 287 cases from 11 hospitals were used for training and evaluation. The overall sensitivity to detect MYC rearrangement was 0.93 and the specificity 0.52, showing that prediction of MYC translocation based on morphology alone was possible in 93% of MYC-rearranged cases. This would allow a simple and fast prescreening, saving approximately 34% of genetic tests with the current algorithm.