bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of the French version of the McLean Screening Instrument for Adolescent Borderline Personality Disorder (MSI-BPD)
Authors: Mirkovic Bojan , Speranza Mario , Cailhol Lionel , Guelfi Julien-Daniel , Perez-Diaz Fernando , Corcos Maurice , Robin Marion , Pham-Scottez Alexandra 
Published: May 2020

Abstract
Background: The study examines the psychometric properties of the French version of the McLean Screening Instrument for Borderline Personality Disorder (MSI-BPD) created by M. Zanarini to screen borderline personality disorder in clinical and non-clinical populations.Method: In this multicentric longitudinal study from the European Network on Borderline Personality Disorder, a sample of 84 adolescent patients from five psychiatric centres and 85 matched controls without psychiatric comorbidity completed the MSI-BPD, French version, and were interviewed with the Structured Interview for DSM-IV Personality (SIDP-IV), in order to assess the presence or absence of borderline personality disorder.Results: The MSI-BPD showed excellent internal consistency (α = 0.87 [0.84;0.90]). Compared to the semi-structured reference interview (SIDP-IV), the MSI-BPD showed substantial congruent validity (AUC = 0.93, CI 95%: 0.90 - 0.97). The optimal cut-off point in the present study was 5 or more, as it had relatively high sensitivity (0.87) and specificity (0.85). In our sample, the cut-off point (7 or more) proposed by the original developers of the MSI-BPD showed high specificity (0.95) but low sensitivity (0.63).Conclusions: The French version of the MSI-BPD is now available, and its psychometric properties are satisfactory. The French version of the MSI-PBD can be used as a screening tool for borderline personality disorder, for clinical purposes or in research studies.
Should we keep hope? Key 8 weeks - Spatiotemporal epidemic characteristics of COVID-19 in Sichuan Province and its comparative analysis with other provinces in China and global epidemic trend
Authors: Xu Xinyin , Zeng Jing , Liu Runyou , Liu Yang , Zhou Xiaobo , Zhou Lijun , Dong Ting , Cha Yuxin , Wang Zhuo , Deng Ying , Zhang Yu , Feng Liao , Pu Chen , Wu Xianping , Zhong Bo 
Published: May 2020

Abstract
Background: To compare the epidemiological characteristics of Sichuan Province, other provinces in China and the world epidemic trends by analyzing the prevalence and length of epidemic time. in order to provide a basis for responding to imported cases abroad and to formulate prevention and control strategies in areas that are still rapidly circulating.Methods: The number of confirmed cases, daily growth, incidence and the length of time from the first reported case to the end of local case(non-overseas imported cases) were compared by spatial and temporal (geographical, temporal) classification. Visualizing the development and changes of epidemic situation by layer through maps.Results: In the first wave, total of 539 cases were reported in Sichuan Province, with the incidence rate of 0.6462 / 100,000. The closer to Hubei, the heavier the epidemic. The peak of Sichuan Province came earlier and the value was lower. Eight weeks after Wuhan lockdown, all became better. The longest epidemic length in city level of China was 53 days, median 23 days. It was released quickly in the 1st month, and accelerated in the 2ed month (three times of 1st month). Most countries outside China began to rise rapidly 4 weeks after their first case. Some European countries was earlier than USA. Germany, Spain, Italy, and China cost 28, 29, 34, and 18 days to reached the peak of daily increment, after their daily increase up to 20 cases. Countries in African Region and South-East Asia Region were at the early stage, in Eastern Mediterranean Region and Region of the Americas were at rapid growth phase,in European presented an inflection point or at a plateau period but falling slowly.Conclusions: Adopting appropriate isolation and control measures is necessary to actively respond to the epidemic situation. If effective measures were implemented at the 8 key weeks, the peak value of the confirmed cases will be lower and decrease quickly. Some countries with improved epidemic situations also need to develop a continuous "local strategy at entry checkpoints" to respond to a possible second local epidemic.
Whole exome sequencing identifies novel DYT1 dystonia-associated genome variants as potential disease modifiers
Authors: Hu Chih-Fen , Luxton G. W. Gant , Lee Feng-Chin , Hsu Chih-Sin , Huang Shih-Ming , Hong Jau-Shyong , Wu San-Pin 
Published: May 2020

Abstract
Background：DYT1 dystonia is a neurological movement disorder characterized by painful sustained muscle contractions resulting in abnormal twisting and postures. In a subset of patients, it is caused by a loss-of-function mutation (ΔE302/303; or ΔE) in the luminal ATPases associated with various cellular activities (AAA+) protein torsinA encoded by the TOR1A gene. The low penetrance of the ΔE mutation (~30-40%) suggests the existence of unknown genetic modifiers of DYT1 dystonia. Results：To identify these modifiers, we performed whole exome sequencing (WES) of blood leukocyte DNA isolated from two DYT1 dystonia patients, three asymptomatic carriers of the ΔE mutation, and an unaffected adult relative. A total of 264 DYT1 dystonia-associated variants (DYT1 variants) were identified in 195 genes. Consistent with the emerging view of torsinA as an important regulator of the cytoskeleton, endoplasmic reticulum homeostasis, and lipid metabolism, we found DYT1 variants in genes that encode proteins implicated in these processes. Moreover, 40 DYT1 variants were detected in 32 genes associated with neuromuscular and neuropsychiatric disorders. Conclusion： The DYT1 variants described in this work represent exciting new targets for future studies designed to increase our understanding of the pathophysiology and pathogenesis of DYT1 dystonia.
LncRNA CAIF Upregulates miR-16 Through Methylation to Suppress LPS-Induced Apoptosis of Cardiomyocytes
Authors: Wang Yan , Zhang Yi 
Published: May 2020

Abstract
Background: Both lncRNA CAIF and miR-16 can inhibit LPS-induced inflammatory response. It is known that LPS plays crucial roles in sepsis. This study was therefore carried out to investigate the interactions between CAIF and miR-16 in sepsis.Methods: Levels of CAIF (A) and miR-16 (B) in plasma samples from sepsis patients (n = 60) and healthy controls (n = 60) were measured by performing RT-qPCR. Correlations of levels of CAIF and miR-16 across plasma samples from sepsis patients (A) and healthy controls (B) were analyzed by linear regression.The effects of CAIF overexpression on miR-16 (B), and the effects of miR-16 overexpression on CAIF (C) were analyzed by RT-qPCR.Results: We found that CAIF and miR-16 were downregulated in plasma of sepsis patients and they were positively correlated. In cardiomyocytes, LPS treatment led to downregulated CAIF and miR-16. Moreover, CAIF overexpression led to upregulated miR-16 and increased methylation of miR-16. However, miR-16 overexpression failed to significantly affect CAIF expression. Cell apoptosis analysis showed that CAIF and miR-16 overexpression suppressed LPS-induced apoptosis of cardiomyocytes. The combination of CAIF and miR-16 overexpression showed stronger effect.Conclusion: CAIF may upregulate miR-16 through methylation to suppress LPS-induced apoptosis of cardiomyocytes.
Perceptions of Important Outcomes of Ethics Case Reflection Rounds: A Qualitative Study among Healthcare Professionals in Childhood Cancer Care
Authors: Weiner Charlotte , Pergert Pernilla , Molewijk Bert , Castor Anders , Bartholdson Cecilia 
Published: May 2020

Abstract
BACKGROUND: In childhood cancer care, healthcare professionals mustdeal with several difficult moral situations in clinical practice. Previous studies show that morally difficult challenges are related to decisions on treatment limitations, infringing on the child's integrity and growing autonomy, and interprofessional conflicts. Research also shows that healthcare professionals want ethics support to help them deal with morally difficult situations.Ethics case reflection (ECR)roundsare one example of ethics support. However, there is little research intohealthcare professionals’ perceptions of important outcomes prior to participation in ethics case reflectionrounds in childhood cancer care. The aim was to explorehealthcare professionals’ perceptions of outcomes of ECR rounds important for handling moral challenges prior to participation in ECR rounds in childhood cancer care.METHODS: This study is based on qualitative data. Healthcare professionals, mostly representing registered nurses, nursing assistants and physicians,working at childhood cancer care centres in Sweden, were invited to respond to the translated and content-validated European Moral Case Deliberation questionnaire,before participating in regular ECR rounds. The main open-ended question included in the questionnaire was analysed according to systematic text condensation. RESULTS: Data was collected from 161 responses from the healthcare professionals who were invited to participate. The responses included healthcare professionals’ perceptions of which ethics case reflection round-related outcomes they found important for handling moral challenges. Three different themes of important outcomes emerged from the analysis of the data: Inter-professional wellbeing, Being in a professional comfort zone, and Improved quality of care. The themes are related to teams, individuals and care, respectively.CONCLUSIONS: Healthcare professionals in childhood cancer care considered it important that ethics support could enhance the well-being of interprofessional teams, support healthcare professionals on an individual level and improve quality of care. To realize these perceived important outcomes, conditions for ECR rounds need to be improved. The results of this study can be used in future training for facilitators of ECR rounds, as well as providing information to healthcare managers wishing to implement this kind of ethics support.
A high-throughput drug combination screen identifies an anti-glioma synergism between MTH1 and PI3K inhibitors
Authors: Chen Zhen , Zhou Tingting , Chen Chao , Duan Chao , Wang Qianqian , Zhou Xiaohui , Zhang Xia , Wu Fangrong , Hua Yunfen , Lin Fan 
Published: May 2020

Abstract
Background
Glioblastoma multiforme (GBM) is the most common and lethal type of primary brain tumor. More than half of GBMs contain mutation(s) of PTEN/PI3K/AKT, making inhibitors targeting the PI3K pathway very attractive for clinical investigation. However, so far, PI3K/AKT/mTOR inhibitors have not achieved satisfactory therapeutic effects in clinical trials of GBM. In this study, we aimed to develop a high-throughput screening method for high-throughput identification of potential targeted agents that synergize with PI3K inhibitors in GBM.
Methods
A Sensitivity Index (SI)-based drug combination screening method was established to evaluate the interactions between BKM120, a pan-PI3K inhibitor, and compounds from a library of 606 target-selective inhibitors. Proliferation, colony and 3D spheroid formation assays, western blotting, comet assay, γ-H2AX staining were used to evaluate the anti-glioma effects of the top-ranked candidates. The drug combination effects were analyzed by the Chou-Talalay method.
Results
Six compounds were successfully identified from the drug screen, including three previously reported compounds that cause synergistic antitumor effects with PI3K/mTOR inhibitors. TH588, an MTH1 inhibitor exhibited significant synergy with BKM120 in suppressing the proliferation, colony formation and 3D spheroid formation of GBM cells. Further investigation revealed that both DNA damage and apoptosis were markedly enhanced upon combination treatment with TH588 and BKM120. Finally, activation of PI3K or overexpression of AKT compromised the anti-glioma efficacy of MTH1 inhibition.
Conclusions
The screening method developed in this study demonstrated its usefulness in the rapid identification of synergistic drug combinations of PI3K inhibitors and targeted agents.
Biological evaluation of [18F]AIF-NOTA-NSC-GLU as a PET tracer for hepatocellular carcinoma
Authors: Lin Liping , Xiang Xianhong , Su Shu , Liu Shaoyu , Xiong Ying , Ma Hui , Yuan Gongjun , Nie Dahong , Tang Ganghua 
Published: May 2020

Abstract
Purpose 18F-labeled amino acids (AAs) as tumor-specific imaging agents play a critical role in hepatocellular carcinoma (HCC) imaging. In this work, we evaluated the synthesis and biological properties of a simple 18F-labeled glutamate analogue, [18F]AIF-1,4,7-triazacyclononane-1,4,7-triacetic-acid-2-S-(4-isothiocyanatobenzyl))-l-glutamate ([18F]AIF-NOTA-NSC-GLU) for HCC imaging via one-step reaction sequence. Methods [18F]AIF-NOTA-NSC-GLU was synthesized via the one-step reaction sequence from NOTA-NSC-GLU. In order to investigate the imaging value of [18F]AIF-NOTA-NSC-GLU in HCC, we conducted PET/CT imaging and competitive binding of [18F]AIF-NOTA-NSC-GLU in human Hep3B 2.1-7 tumor-bearing mice. The transport mechanism of [18F]AIF-NOTA-NSC-GLU was determined by competitive inhibition and protein incorporation experiments in vitro. Results [18F]AIF-NOTA-NSC-GLU was prepared with decay-corrected radiochemical yield of 29.3 ± 5.6% (n=10) within 20 min. In vitro competitive inhibition experiments demonstrated that Na+-dependent Systems XAG-, B0+, ASC and minor XC- were involved in the uptake of [18F]AIF-NOTA-NSC-GLU, with Na+-dependent System XAG- possibly playing a more dominant role. Protein incorporation studies of the Hep3B 2.1-7 human hepatoma cell line found almost no protein incorporation. Micro-PET/CT imaging with [18F]AIF-NOTA-NSC-GLU showed good tumor-to-background contrast in Hep3B 2.1-7 human hepatoma-bearing mouse models. After [18F]AIF-NOTA-NSC-GLU injection, the tumor-to-liver uptake ratio of [18F]AIF-NOTA-NSC-GLU reached the peak at 30 min post-injection (2.06 ± 0.17). In vivo competitive binding experiments exhibited that the tumor-to-liver uptake ratio decreased by the addition of the inhibitors to block the system XAG-. Conclusion We have successfully synthesized [18F]AIF-NOTA-NSC-GLU as a novel PET tracer with good radiochemical yield and high radiochemical purity. Our findings indicate that [18F]AIF-NOTA-NSC-GLU might have good clinical potential as a PET tumor-detecting agent for HCC imaging.
Sleep Characteristics and Problems of 2-year-olds with Williams Syndrome: Relations with Language and Behavior
Authors: de Magalhães Caroline Greiner , O'Brien Louise M. , Mervis Carolyn B. 
Published: May 2020

Abstract
Background
Sleep problems have been shown to have a negative impact on language development and behavior for both typically developing children and children with a range of neurodevelopmental disorders. The relation of sleep characteristics and problems to language and behavior for children with Williams syndrome (WS) is unclear. The goal of this study was to address these relations for 2-year-olds with WS. Associations of nonverbal reasoning ability, nighttime sleep duration, and excessive daytime sleepiness with language ability and behavior problems were considered.
Method:
Ninety-six 2-year-olds with genetically confirmed classic-length WS deletions participated. Parents completed the Pediatric Sleep Questionnaire, which includes a Sleep Related Breathing Disorder (SRBD) scale with a subscale measuring excessive daytime sleepiness, to assess sleep characteristics and problems. Parents also completed the Child Behavior Checklist (CBCL) and the MacArthur-Bates Communicative Development Inventory: Words and Sentences to assess behavior problems and expressive vocabulary, respectively. Children completed the Mullen Scales of Early Learning to measure nonverbal reasoning and language abilities.
Results
Parents indicated that children slept an average of 10.36 hours per night (SD = 1.09, range: 7.3–13.3), not differing significantly from the mean reported by Bell and Zimmerman (2010) for typically developing toddlers (p = .787). Sixteen percent of participants screened positive for SRBD and 30% for excessive daytime sleepiness. Children who screened positive for SRBD had significantly more behavior problems on all CBCL scales than children who screened negative. Children with daytime sleepiness had significantly more attention/hyperactivity, stress, and externalizing problems than those who did not have daytime sleepiness. Individual differences in parent-reported nighttime sleep duration and directly measured nonverbal reasoning abilities accounted for unique variance in expressive language, receptive language, and internalizing problems. Individual differences in parent-reported daytime sleepiness accounted for unique variance in externalizing problems.
Conclusions
The relations of nighttime sleep duration, positive screens for SRBD, and excessive daytime sleepiness to language and behavior in toddlers with WS parallel prior findings for typically developing toddlers. These results highlight the importance of screening young children with WS for sleep problems. Studies investigating the efficacy of behavioral strategies for improving sleep in children with WS are warranted.
Detecting Melanoma Antigen Family A3 Expression in Solid/Non-Solid Human Tumor
Authors: Jiang Haoran , Zhao Lihao , Yang Han , Zhao Mengjing , Duan Yuxia , Li Gang 
Published: May 2020

Abstract
Background: MAGEA3 is a member of melanoma antigen family and has been recognized to express in many types of human cancers recently. In spite of the development of cancer vaccine, the prognostic value of MAGEA3 has not been well evaluated, due to the variability of clinical data and lack of clinical trials on the prognostic values.Method: Studies that evaluated MAGEA3 expression with a follow-up for at least 36 months were selected by searching in PubMed, WOS, Cochrane library, Embase. Published data was recorded and calculated into odds ratios (OR) for mortality in three or five years with Mantel-Haenszel random-effect model. Results: 11 studies were selected. The median positive rate was 45%. MAGEA3 always combines with worse survival on three or five years survival. The correlation between MAGEA3 and squamous carcinoma seemed stronger than adenocarcinoma on three-year OS while things got a reverse when it came to five-year OS. Most importantly, we found that all solid tumors originated from endoderm seemed to enjoy a strongest correlation among all the three germ layers.Conclusion: In this meta-analysis, we found that the expression of MAGEA3 can connect with worse outcome, and it probably can be a predictor for patients’ prognosis in clinical practice.
Seasonal Trends in Global Dieting Online: A Big Data Survey
Authors: Park Myung-Bae , Wang Jumee , Bulwer Bernard E. , Ranabhat Chhabi 
Published: May 2020

Abstract
Background

We aimed to explore whether the massive amounts of data generated during online search interest in dieting and weight loss could be harnessed, using big data analysis, with a view to its potential incorporation in global health obesity prevention efforts.
Methods

We applied big data analysis to the major global health practice of dieting for weight management. Data was collected from Google and Naver search engines from January 2004 to January 2018 using the search term ‘diet’, in: A) selected six Northern and Southern Hemisphere countries, B) five primarily Arab and Muslim countries grouped as (i) conservative, (ii) semi-conservative, and (iii) liberal, and C) South Korea.
Results

Using cosinor analysis to evaluate the periodic flow of time series data, we found that global searches and interest in dieting and weight loss appeared to be seasonal (seasonality amplitude = 6.94, CI = 5.33 ~ 8.56, P > 0.0000), highest in April and the lowest in October for both Northern and Southern Hemisphere countries (seasonality amplitude for Northern Hemisphere = 6.68, CI = 5.13 ~ 8.22, P > 0.0000), with a different seasonal dieting trend generally seen in the Arab and Muslim countries (monthly seasonal seasonality (amplitude = 4.07, CI = 2.20 ~ 5.95, P > 0.0000).
Conclusions

Our findings indicate that big data analysis of social media can be harnessed as an adjunct tool for addressing important public health issues related to diet, weight loss, and obesity management including the optimal timing for healthy public interventions, and the avoidance of food fads and quackery.
Magnitude and associated factors of disability in patients with schizophrenia attending Gondar, Felegehiwot and Debretabor hospitals Amhara North West Ethiopia 2019: Institutional based cross-sectional study.
Authors: Mihretie Getnet Beyene , Legas Getasew Molla , Asnakew Sintayehu Alemayehu , Azale Telake Bisetegn , Abera Mebrat Woldesenbet 
Published: May 2020

Abstract
Background Schizophrenia is a severe, chronic and disabling mental illness that places significant burden not only on the individuals, but also on their families and society. As per our knowledge there is no study conducted on prevalence and associated factors of disability in schizophrenic patients in Ethiopia. Their fore, this study aimed to assess the magnitude and associated factors of disability in patients with schizophrenia attending Gondar, Felegehiwot and Debretabor hospital, North West Ethiopia 2019Methods An Institutional -based cross-sectional study was conducted among schizophrenic patients attending Gondar, Felegehiwot and Debretabor hospitals Amhara Ethiopia. A total of 420 participants were included in the study. Data were collected by face to face interview using systematic random sampling method. Disability was measured using World Health Organization Disability Assessment Scale 2.0. Coded variables were entered into Epi data V.4.4.2 and exported to SPSS V.24 for analysis. Bivariate and multivariate logistic regressions with OR and 95% CI were employed. The level of significance of association was determined at p-value < 0.05.Result A total of 420 participants were interviewed, with a response rate of 99.3%. The magnitude of disability in this study was 41.7% (95%CI: 36.9, 46.4). Current use of alcohol (AOR = 2.47, 95CI;1.36,4.48), being jobless(AOR = 3.27, 95CI; 1.80, 5.93)., had negative symptoms (AOR = 13.05,95CI,7.58,22.45), had 5 and more years of illness(AOR = 2.75,95CI;1.29,5.86) and had 5 and more years of untreated psychosis (AOR = 3.78,95CI, 1.85,7.75) were predictors of disability.Conclusion and recommendation: The magnitude of disability in this study was high. Avoidance of alcohol usage, early initiation of treatment, creating job opportunity and giving special emphasis for patients having negative symptoms is recommended.