bluuebunny/crossref_metadata_2025_split huggingface dataset:
Latexin deficiency in mice up-regulates inflammation and aggravates colitis through HECTD1/Rps3/NF-κB pathway
Authors: Li Yaping , Huang Baohua , Yang Hua , Kan Shuang , Yao Yanling , Liu Xin , Pu Shiming , He Guozhang , Khan Taj-Malook , Qi Guangying , Zhou Zuping , Shu Wei , Chen Ming 
Published: June 2020

AbstractThe function of Latexin (LXN) in inflammation has attracted attention. However, no data are available regarding its role in colitis. We report that LXN is a suppressor of colitis. LXN deficiency leads to the severity of colitis in DSS-induced mice, and LXN is required for the therapeutic effect of retinoic acid on colitis. Using a proteomics approach, we demonstrate that LXN interacts and forms a functional complex with HECTD1 (an E3 ubiquitin ligase) and ribosomal protein subunit3 (Rps3). IκBα is one of the substrates of HECTD1. Ectopic expression of LXN leads to IκBα accumulation in intestinal epithelial cells, however, LXN knockdown enhances the interaction of HECTD1 and Rps3, contributing to the ubiquitination degradation of IκBα, and subsequently enhances inflammatory response. Thus, our findings provided a novel mechanism underlying LXN modulates colitis via HECTD1/Rps3/NF-κB pathway and significant implications for the development of novel strategies for the treatment of colitis by targeting LXN.
Point-of-care detection, characterization, and removal of chocolate bloom using a handheld Raman spectrometer
Authors: Heuler Joshua , He Siyu , Ambardar Sharad , Voronine Dmitri V. 
Published: June 2020

AbstractChocolate bloom is an off-white coating on the surface of chocolate products due to the altered distribution of the ingredients. Bloom reduces the shelf-life of chocolate and affects its visual and tactile quality, all of which are serious concerns for chocolate manufacturers and consumers. The automated, rapid, and noninvasive point-of-care detection of chocolate bloom has been an essential but challenging problem. The ability to detect and characterize chocolate bloom using portable laser spectroscopy could be used to develop in-situ quality control sensors. In this work, a handheld Raman spectrometer was used to detect chocolate bloom. Raman spectra acquired from bloomed HERSHEY’S milk chocolate, Hawaiian Host milk chocolate covered macadamia nuts, and Babayevsky Russian dark chocolate were used to characterize the type of bloom. The 1064 nm laser beam of the handheld Raman instrument was used to partially remove the fat bloom of the dark chocolate and to induce sugar bloom on the milk chocolate. The handheld Raman approach has a high potential for industrial and consumer applications for the on-site chemical analysis of chocolate bloom and as an alternative laser-based chocolate decoration.
Identification of core genes in the progression of endometrial cancer and cancer cell-derived exosomes by an integrative analysis
Authors: Shi Shuang , Tan Qiang , Feng Fuqiang , Huang Heping , Liang Jingjie , Cao Dingren , Wang Zhengguang 
Published: June 2020

AbstractEndometrial cancer is one of the most prevalent tumors of the female reproductive system causing serious health effects to women worldwide. Although numerous studies, including analysis of gene expression profile and cellular microenvironment have been reported in this field, pathogenesis of this disease remains unclear. In this study, we performed a system bioinformatics analysis of endometrial cancer using the Gene Expression Omnibus (GEO) datasets (GSE17025, GSE63678, and GSE115810) to identify the core genes. In addition, exosomes derived from endometrial cancer cells were also isolated and identified. First, we analyzed the differentially expressed genes (DEGs) between endometrial cancer tissues and normal tissues in clinic samples. We found that HAND2-AS1, PEG3, OGN, SFRP4, and OSR2 were co-expressed across all 3 datasets. Pathways analysis showed that several pathways associated with endometrial cancer, including “p53 signaling pathway”, “Glutathione metabolism”, “Cell cycle”, and etc. Next, we selected DEGs with highly significant fold change and co-expressed across the 3 datasets and validated them in the TCGA database using Gene Expression Profiling Interactive Analysis (GEPIA). Finally, we performed a survival analysis and identified four genes (TOP2A, ASPM, EFEMP1, and FOXL2) that play key roles in endometrial cancer. We found up-regulation of TOP2A and ASPM in endometrial cancer tissues or cells, while EFEMP1 and FOXL2 were down-regulated. Furthermore, we isolated exosomes from the culturing supernatants of endometrial cancer cells (Ishikawa and HEC-1-A) and found that miR-133a, which regulates expression of FOXL2, were present in exosomes and that they could be delivered to normal endometrial cells. The common DEGs, pathways, and exosomal miRNAs identified in this study might play an important role in progression as well as diagnosis of endometrial cancer. In conclusion, our results provide insights into the pathogenesis and risk assessment of endometrial cancer. Even so, further studies are required to elucidate on the precise mechanism of action of these genes in endometrial cancer.
Coexistence of valley polarization and Chern insulating states in MoS2 monolayers with n-p codoping
Authors: Wei Xinyuan , Zhang Jiayong , Zhao Bao , Yang Zhongqin 
Published: June 2020

AbstractThe electronic and topological properties of MoS2 monolayers with n-p codoping effect are investigated by using first-principles calculations. Two types of the doped Nb atoms play the roles of the p-type and n-type dopants, respectively. The n-p codoping is found inducing a large valley polarization, associated with the strong magnetization induced by the Nb dopants. Interestingly, the system simultaneously owns a perfect Chern insulating band gap opened exactly at the Fermi level. The nontrivial band gap comes from the lifting of the degeneracy of the dxz and dyz orbitals of Nb2 atoms after the spin-orbit coupling is considered. Our work inspires exciting prospects to tune the novel properties of materials with n-p codoping effects.
Spatio-temporal dynamics of arbuscular mycorrhizal fungi and soil organic carbon in coastal saline soil of China
Authors: Zhang Huan-Shi , Zhou Ming-Xi , Zai Xue-Ming , Zhao Fu-Geng , Qin Pei 
Published: June 2020

AbstractA comprehensive understanding of the relationship between arbuscular mycorrhizal (AM) fungi and coastal saline soil organic carbon (SOC) is crucial for analysis of the function of coastal wetlands in soil carbon sequestration. In a field experiment, the temporal and spatial dynamics of AM fungi, glomalin-related soil protein (GRSP) – which is described as a N-linked glycoprotein and the putative gene product of AM fungi, SOC, and soil aggregates were investigated in halophyte Kosteletzkya virginica rhizosphere soil of coastal saline areas of North Jiangsu, China. Soil samples were collected from a depth of up to 30 cm in two plantation regions from August 2012 to May 2013. Results showed K. virginica formed a strong symbiotic relationship to AM fungi. AM colonization and spore density were the highest in the 10–20 cm soil layer of Jinhai farm in August 2012, because of the presence of numerous fibrous roots in this soil layer. The total GRSP and SOC were the highest in the 0–10 cm soil layer in May 2013 and November 2012, respectively. Correlation coefficient analysis revealed that AM colonization and spore density were positively correlated with total GRSP. Meanwhile, total GRSP was significantly positively correlated with large macroaggregates (>3 mm), SOC, total P, Olsen P, and soil microbial biomass carbon (SMBC), but negatively correlated with microaggregates (<0.25 mm), soil EC, total N, and pH. SOC was positively correlated with spore density, large macroaggregates, small macroaggregates (2–0.25 mm), alkaline N, and SMBC and negatively correlated with microaggregates, EC, pH, and total K. Although it may be a statistical artifact, we found an interesting phenomenon that there was no significant correlation between soil aggregates and AM colonization or spore density. Hence, total GRSP is a vital source of saline soil C pool and an important biological indicator for evaluating coastal saline SOC pool and soil fertility, while AM colonization or spore density may not be.
Hollow fiber-combined glucose-responsive gel technology as an in vivo electronics-free insulin delivery system
Authors: Matsumoto Akira , Kuwata Hirohito , Kimura Shinichiro , Matsumoto Hiroko , Ochi Kozue , Moro-oka Yuki , Watanabe Akiko , Yamada Hironori , Ishii Hitoshi , Miyazawa Taiki , Chen Siyuan , Baba Toshiaki , Yoshida Hiroshi , Nakamura Taichi , Inoue Hiroshi , Ogawa Yoshihiro , Tanaka Miyako , Miyahara Yuji , Suganami Takayoshi 
Published: June 2020

AbstractAccumulating evidence demonstrates that not only sustained elevation of blood glucose levels but also the glucose fluctuation represents key determinants for diabetic complications and mortality. Current closed-loop insulin therapy option is limited to the use of electronics-based systems, although it poses some technical issues with high cost. Here we demonstrate an electronics-free, synthetic boronate gel-based insulin-diffusion-control device technology that can cope with glucose fluctuations and potentially address the electronics-derived issues. The gel was combined with hemodialysis hollow fibers and scaled suitable for rats, serving as a subcutaneously implantable, insulin-diffusion-active site in a manner dependent on the subcutaneous glucose. Continuous glucose monitoring tests revealed that our device not only normalizes average glucose level of rats, but also markedly ameliorates the fluctuations over timescale of a day without inducing hypoglycemia. With inherent stability, diffusion-dependent scalability, and week-long & acute glucose-responsiveness, our technology may offer a low-cost alternative to current electronics-based approaches.
Teichoic acids anchor distinct cell wall lamellae in an apically growing bacterium
Authors: Ultee Eveline , van der Aart Lizah T. , Zhang Le , van Dissel Dino , Diebolder Christoph A. , van Wezel Gilles P. , Claessen Dennis , Briegel Ariane 
Published: June 2020

AbstractThe bacterial cell wall is a multicomponent structure that provides structural support and protection. In monoderm species, the cell wall is made up predominantly of peptidoglycan, teichoic acids and capsular glycans. Filamentous monoderm Actinobacteria incorporate new cell-wall material at their tips. Here we use cryo-electron tomography to reveal the architecture of the actinobacterial cell wall of Streptomyces coelicolor. Our data shows a density difference between the apex and subapical regions. Removal of teichoic acids results in a patchy cell wall and distinct lamellae. Knock-down of tagO expression using CRISPR-dCas9 interference leads to growth retardation, presumably because build-in of teichoic acids had become rate-limiting. Absence of extracellular glycans produced by MatAB and CslA proteins results in a thinner wall lacking lamellae and patches. We propose that the Streptomyces cell wall is composed of layers of peptidoglycan and extracellular polymers that are structurally supported by teichoic acids.
The predictive value of serum lipids for eye metastases in male nasopharyngeal carcinoma patients
Authors: Xie Zhen , Shao Yi 
Published: June 2020

Abstract
Background: Nasopharyngeal carcinoma (NPC) is a tumor that is commonly found in southern China. NPC has several risk factors, such as infection with the Epstein–Barr virus. However, we know little about the risk factors for eye metastasis (EM) in male patients with NPC. Serum lipids are well recognized as risk factors for cardiovascular disease, and recent studies show that they also have a relationship with the development of NPC.
Purpose: We designed the present study to determine whether they were relevant with the development of EM in male NPC patients by detecting the levels of several serum lipids.
Methods: A total of 1140 male patients with NPC were enrolled in this retrospective study and we divided them into two groups: the metastasis (EM) group and non-eye metastasis (NEM) group. A variety of serum lipids between the two groups were tested and compared.
Results: There were statistical differences in the levels of serum TG and TC between these two groups. Binary logistic regression showed that TG and TC were independent risk factors for EM in male NPC patients with P=0.004 and P&lt;0.001, respectively. The area under the curve of TG and TC were 0.764 and 0.681, respectively, using cutoff values of 0.975 and 3.425 mmol/l, respectively. We found that TG had higher sensitivity and specificity values with 87.5% and 62.7%, respectively, than TC which were 50.0% and 87.2%.
Conclusion: TG and TC are potential risk factors for eye metastases in male NPC patients.
MiR-96-5p alleviates inflammatory responses by targeting NAMPT and regulating the NF-κB pathway in neonatal sepsis
Authors: Chen Xueli , Chen Ying , Dai Li , Wang Na 
Published: June 2020

Abstract
Neonatal septicemia is a serious infectious disease in the neonatal period. MicroRNAs (miRNAs) have been reported to participate in the inflammatory responses in neonatal sepsis. The aim of the present study was to explore the effects and molecular mechanism of miR-96-5p on regulating the inflammatory responses in neonatal sepsis. MiR-96-5p was low expressed while nicotinamide phosphoribosyltransferase (NAMPT) was high expressed in the serum of neonatal septicemia patients. The expression of miR-96-5p was decreased in LPS-induced inflammatory responses. Besides, miR-95-5p relieved LPS-induced inflammatory responses in RAW264.7 cells. NAMPT was demonstrated as a potential target of miR-96-5p, and knockdown of NAMPT reduced inflammatory in RAW264.7 cells stimulated with LPS. Moreover, overexpression of NAMPT reversed the effects of miR-96-5p on LPS-induced inflammatory responses. In addition, miR-96-5p inhibited nuclear factor (NF)-κB signaling pathway in RAW264.7 cells stimulated with LPS. MiR-96-5p alleviated inflammatory responses via targeting NAMPT and inhibiting NF-κB pathway in neonatal sepsis.
A ferredoxin-dependent dihydropyrimidine dehydrogenase in Clostridium chromiireducens
Authors: Wang Feifei , Wei Yifeng , Lu Qiang , Ang Ee Lui , Zhao Huimin , Zhang Yan 
Published: June 2020

Abstract
Dihydropyrimidine dehydrogenase (PydA) catalyzes the first step of the reductive pyrimidine degradation (Pyd) pathway in bacteria and eukaryotes, enabling pyrimidines to be utilized as substrates for growth. PydA homologs studied to date catalyze the reduction of uracil to dihydrouracil, coupled to the oxidation of NAD(P)H. Uracil reduction occurs at a flavin mononucleotide (FMN) site, and NAD(P)H oxidation occurs at a flavin adenine dinucleotide (FAD) site, with two ferredoxin domains thought to mediate inter-site electron transfer. Here, we report the biochemical characterization of a Clostridial PydA homolog (PydAc) from a Pyd gene cluster in the strict anaerobic bacterium Clostridium chromiireducens. PydAc lacks the FAD domain, and instead is able to catalyze uracil reduction using reduced methyl viologen or reduced ferredoxin as the electron source. Homologs of PydAc are present in Pyd gene clusters in many strict anaerobic bacteria, which use reduced ferredoxin as an intermediate in their energy metabolism.
Real‐time non‐intrusive RF biochemical sensor
Authors: Pandit Nidhi , Jaiswal Rahul K. , Pathak Nagendra P. 
Published: June 2020

In this Letter, a non‐destructive microwave resonant sensor based on the modified complementary split‐ring resonator has been proposed and analysed with respect to its sensitivity for detecting the aqueous biochemicals. This technique is based on the perturbation theory and only requires to measure resonant frequency and hence, achieving a substantial reduction in the computation time and cost. The proposed sensor is designed to achieve high sensitivity performance with a measured quality factor of the sensor in the bare state, Q B = 631.5, at the centre frequency of f o = 6.31 GHz. The proposed sensor has a maximum sensitivity of S = 6.23 MHz with respect to a unit change in the dielectric constant value. A numerical model is developed for the dielectric constant of the biochemicals as a function of the resonant frequency. The proposed sensor, therefore, provides high sensitivity, simple structure layout and real‐time response with cost effectiveness. The proposed structure can be a potential candidate for the non‐intrusive sensing of aqueous biochemicals.
Real‐time detection of vulnerable power system areas to geomagnetic disturbance
Authors: Rezaei‐Zare Mina , Ariannik Mohamadreza , Rezaei‐Zare Afshin 
Published: June 2020

This study proposes an approach for the fast and accurate identification of the vulnerable areas of the power system to geomagnetic disturbance (GMD). The proposed method can be used for real‐time situational awareness and preparedness in power system control rooms and proactive mitigation of the GMD threats. In addition, it can be employed as an off‐line GMD vulnerability assessment tool for power system planning analysis. In this study, a generalised mathematical basis is presented to find the maximum geomagnetically induced current (GIC) flow in the multi‐zone earth structure based on the orthogonal GIC components. Furthermore, a real‐time frequency estimation method is developed based on wavelet transform to estimate the frequency of the geomagnetic waveform for the maximum GIC calculation. The proposed approach is applied to the Ontario 500 and 230 kV transmission systems to identify the vulnerable equipment. The results are also compared with the angle sweep results. The numerical results reveal that not only the proposed method is more accurate but also significantly faster than the angle sweep method. Such prominent features introduce the proposed approach as a preferable method for real‐time applications and optimisation of power system operation during GMDs.
V363 Cassiopeiae: a new lithium-rich Galactic Cepheid
Authors: Catanzaro G. , Ripepi V. , Clementini G. , Cusano F. , De Somma G. , Leccia S. , Marconi M. , Molinaro R. , Moretti M. I. , Musella I. , Testa V. 
Published: June 2020

Context. Classical Cepheids (DCEPs) are important astrophysical objects not only as standard candles in the determination of the cosmic distance ladder, but also as a testbed for the stellar evolution theory. This is based on the strict connection between their pulsation (period(s) and amplitudes) and stellar parameters (luminosity, mass, effective temperature, and metallicity).
Aims. We examine the nature of the Galactic DCEP V363 Cas and other DCEPs that show cosmic abundances of lithium in their atmospheres.
Methods. We collected three epochs of high-resolution spectroscopy for V363 Cas with HARPS-N at the TNG. We measured accurate stellar parameters: the effective temperatures, gravities, microturbulences, radial velocities, and metal abundances.
Results. We detected a lithium abundance of A(Li) = 2.86 ± 0.10 dex, along with iron, carbon, and oxygen abundances of [Fe/H] = −0.30 ± 0.12 dex, [C/H] = −0.06 ± 0.15 dex, and [O/H] = 0.00 ± 0.12 dex. V363 Cas is the fifth of the Milky Way DCEPs to exhibit a Li-rich feature. An analysis of historical time-series spanning a 100-year interval shows that the period of V363 Cas is increasing, with a sharp acceleration after HJD = 2 453 000 days. This is a clear indication of a first crossing of the instability strip.
Conclusions. Our results favour the scenario in which the five Galactic Li-rich DCEPs are on their first crossing of the instability strip and have had slowly rotating progenitors during their main-sequence phase.
Primär- und Sekundärprävention des Multiplen Myeloms
Authors: Cordas dos Santos David , Erickson Nicole , Gerland Lars , Jundt Franziska , Theurich Sebastian 
Published: June 2020

AbstractLifestyle factors such as diet, physical activity and exposure to noxious agents are modifiable factors that have a significant impact on the state of health and life expectancy of humans. The following article is intended to provide an overview of current knowledge on the influence of these lifestyle factors on the development and progression of multiple myeloma and is dedicated to the question of the extent to which prevention strategies can be usefully applied.
Rezidivtherapie des Multiplen Myeloms – Individualisierte Konzepte aus dem Arsenal vielfältiger Optionen
Authors: Weyermann Charlotte , Straka Christian , Einsele Hermann 
Published: June 2020

AbstractThe prognosis of patients with multiple myeloma has improved significantly over the past 20 years. However, the patient population in the relapse situation is very heterogeneous due to increasing age and the previous course of the disease and therapy. In particular, the approval of new targeted substances offers numerous treatment options that can be adapted to the individual situation.In relapsed multiple myeloma, disease- and patient-specific factors must be considered for an individually adapted therapy. Suitable patients can also receive an autologous stem cell transplant (ASCT) or, in the case of early relapse after ASCT, an allogenic stem cell transplant, if possible as part clinical studies. Proteasome or immunomodulator-based triple combinations are the standard in recurrence. In frail patients, a combination of two can also be used. The new substances also offer very good therapeutic options for high-risk cytogenetics or renal insufficiency. The monoclonal antibodies Daratumumab and Elotuzumab are well tolerated except for infusion reactions and are highly effective in various combinations, even in high-risk cytogenetics.
Die Bedeutung des extrathorakalen Luftraumes für die
Kieferorthopädie
Authors: Hourfar Jan , Kinzinger Gero , Lisson Jörg A. 
Published: June 2020

ZusammenfassungDer „Extrathorakale Luftraum“ findet im klinischen Alltag des
Kieferorthopäden sowohl bei der Fallplanung als auch bei der
Therapie kaum Beachtung. Ziel dieses Beitrages ist es deshalb, Anatomie,
Historie, unterschiedliche Definitionen, bildgebende Verfahren sowie
Simulationstechniken des extrathorakalen Luftraumes darzustellen. Ohne
kritische Aspekte zu vernachlässigen, werden therapeutische
Maßnahmen mit möglichen Auswirkungen auf den extrathorakalen
Luftraum, ausgewählte Studienergebnisse sowie
interdisziplinäre Implikationen aufgezeigt, auch um die Relevanz des
Themas für den Kliniker hervorzuheben.
Akademisierung des Hebammenberufs (Teil 2): Risiken – und wie
sie in den Studiengängen bestmöglich vermieden werden können
Authors: Graf Joachim , Simoes Elisabeth , Plappert Claudia F. , Abele Harald 
Published: June 2020

ZusammenfassungDie jetzt auch in Deutschland einsetzende Vollakademisierung der bisher
fachschulisch verorteten Hebammenausbildung stellt sowohl die Länder als
auch die Hochschulen vor große Herausforderungen. Ziel dieses Artikels
ist vor diesem Hintergrund, mögliche Risiken, die sich aus der
Vollakademisierung und der Neufassung des Hebammengesetzes ergeben, zu
identifizieren und mögliche Lösungsstrategien aufzuzeigen, um
der Implementierung von Studiengängen Vorschub zu leisten.