bluuebunny/crossref_metadata_2025_split huggingface dataset:
PEMBUATAN MEDIA PEMBELAJARAN BACA TULIS HITUNG (CALISTUNG) BAGI MASYARAKAT DI DISTRIK ULILIN
Authors: Andi Andi Saparuddin Nur , Nggaruaka Tobias , Kichi Hermansyah Agus 
Published: December 2019

Abstract
The ability of Calistung is a basic knowledge that must be possessed by someone to be able to learn various things. Merauke Regency still has lliteracy problems related to the low ability of Calistung, especially in Baidub Village, Ulilin District. The purpose of this service is to provide training and assistance to the community in Kampung Baidub to be able to improve the ability of Calistung. Service methods used are through the stages of observation, socialization, mentoring training, monitoring and evaluation. The activity will take place in May-August 2019 with the target of the illiterate community and students of Bupul XII Elementary Inpres in Baidub Village, Ulilin District. The results of this dedication were obtained by Calistung learning media in the form of simple counting tools (abacus), picture cards, word boards, and Calistung books. The level of community participation and students participating in the activities is very high as indicated by the ability of the community Calistung and elementary students in Baidub Village to increase after the implementation of the service.
Abstrak
Kemampuan Calistung merupakan pengetahuan dasar yang harus dimiliki oleh seseorang untuk dapat mempelajari berbagai hal. Kabupaten Merauke masih memiliki permasalahan penuntasan buta aksara terkait rendahnya kemampuan Calistung khususnya di Kampung Baidub, Distrik Ulilin. Tujuan dilaksanakannya pengabdian ini adalah untuk memberikan pelatihan dan pendampingan kepada masyarakat di Kampung Baidub agar mampu meningkatkan kemampuan Calistung. Metode pengabdian yang digunakan melalui tahapan observasi, sosialisasi, pelatihan pendampingan, monitoring dan evaluasi. Pelaksanaan kegiatan berlangsung pada Bulan Mei-Agustus 2019 dengan sasaran masyarakat buta aksara dan siswa SD Inpres Bupul XII di Kampung Baidub, Distrik Ulilin. Hasil pengabdian ini diperoleh media pembelajaran calistung berupa alat hitung sederhana (sempoa), kartu bergambar, papan kata, dan buku Calistung. Tingkat partsisipasi masyarakat dan siswa mengikuti kegiatan tergolong sangat tinggi yang ditunjukkan dengan kemampuan Calistung masyarakat dan siswa SD di Kampung Baidub mengalami peningkatan setelah pelaksanaan pengabdian.
Population’s perceptions and availability of primary care services in Romania- a pilot survey in County Brasov.
Authors: Neculau Andrea Elena , Rogozea Liliana , Popa Daniela , Atudorei Ioana , Leasu Florin , Moga Marius Alexandru , Lacatus Anca 
Published: December 2019

Abstract
Background National health reports on Romania show that decisions in healthcare planning are not correlated with the health needs of the population and that there is a high degree of unmet healthcare needs of the population (related to cost, distance and waiting times), especially for low-income populations. Family medicine is facing underfinancing, slow pace of development. Methods The research is part of a wider project of health services needs assessment in county Brasov, Romania. A subset of questions where dedicated to identifying the perception of population on primary healthcare services. Comparison with previous national and international studies was done. Results The characteristics of the population questioned: predominantly women (67.2%), 61.1% graduates of high school or other professional schools. 97.4% are registered with a FD. The average number of visits at the FD is 11.25, higher than the national average of 7.7 reported in a previous study in 2009. Access to the FD is appreciated as satisfactory in terms of availability of doctors in the community (97.4%), opening times by (91.1%), phone access (90.5%). Only 26.6% of participants reported a same day opportunity to reach the FD. Continuity of care is reported as present in 58.7% of cases in out of hours centres. The population is appreciating the fact that FDs knows their history (90%), knows how to treat them (88.2%). Most of the preventative services are offered by FDs. 94.4% flu vaccination, 85.6% pregnancy monitoring, 90.7% well child visits. Procedures like blood draws, pap smear have less availability (46.2% respectively 63.1%). Conclusions Despite limitations in the practice of family medicine in Romania and therefore a narrow spectrum of services offered by primary care in general, the level of contentment of the population with this healthcare resource is still high. Barriers to access are related to the lack of some essential services, especially preventive and out-of-hours services. Research at national level should be done in order to better categorize population’ perceptions on primary care and be able to use their opinion to influence policies and healthcare planning. Key words: unmet health needs, population’s perception, primary care, Romania
Comparison of First-line Pazopanib and Sunitinib in Metastatic Renal Cell Carcinoma: Experiences of the Urologic Cancer Centre for Research and Innovation
Authors: Wong Emily C.L. , Tajzler Camilla , Vasisth Gaurav , Zhu Amanda , Chow Mathilda , Lovatt Catherine , Hotte Sebastien , Kapoor Anil 
Published: December 2019

Abstract
Background: Sunitinib and pazopanib are orally-administered tyrosine kinase receptor inhibitors (TKIs) approved as first-line therapy for the treatment of metastatic renal cell carcinoma (mRCC). The IMDC criteria are a predictive prognostic model for patients with mRCC when stratified into three prognosis groups: favourable, intermediate and poor. We retrospectively compared the efficacy and safety of sunitinib and pazopanib as first-line therapy for patients with mRCC in our single institution database. Methods: Retrospective analysis was done to compare progression-free survival (PFS) and side effects of sunitinib and pazopanib as first-line therapy in patients with mRCC. Patients were stratified into prognosis groups according to IMDC criteria. Disease assessment was performed on measurable aspects of disease based on computed tomography or magnetic resonance imaging reports. Survival analysis was performed using the Kaplan-Meier method and Cox regression, with disease progression as the endpoint.Results: Data was obtained from 228 patients with mRCC who were treated with either pazopanib (n=57) or sunitinib (n=171). No significant difference in PFS was found between sunitinib and pazopanib (HR for disease progression or all-cause death, 1.10; 95%CI: 0.76-1.57, p=0.62). Median PFS time for patients receiving sunitinib was 9.4 months and for pazopanib, 8.5 months. Median PFS for patients with intermediate-risk disease was similar between groups (9.4 months vs. 9.2 months, respectively, p=0.93). However, patients treated with sunitinib experienced a greater number of side effects compared to pazopanib. Conclusions: Sunitinib and pazopanib are similarly efficacious as first-line therapy for mRCC. However, adverse events are lower with pazopanib.
Combined impact of lifestyle factors on low back pain: A cross-sectional study of over 400,000 Japanese adults
Authors: Yoshimoto Takahiko , Ochiai Hirotaka , Shirasawa Takako , Nagahama Satsue , Uehara Akihito , Muramatsu Jun , Kokaze Akatsuki 
Published: December 2019

Abstract
Background: Many epidemiological studies have indicated the association between low back pain (LBP) and lifestyle factors, such as physical activity, smoking, weight gain, diet, and sleep problems. However, the combined effect of lifestyle factors on LBP has not been adequately investigated. Thus, we aimed to investigate the association between a cluster of unhealthy lifestyle behaviors and LBP using a large cohort of Japanese adults.Methods: We included 419,003 adults aged over 20 years who underwent an annual health checkup between April 2013 and March 2014 in Japan. Information on the following lifestyle factors were collected using the standardized questionnaire recommended by the Ministry of Health, Labour and Welfare in Japan: smoking, alcohol intake, exercise, physical activity, walking speed, weight control, eating habits, and sleep. Each factor was evaluated as a dichotomous variable (1: health risk, 0: no health risk). A lifestyle risk score was calculated by summing the score of each lifestyle factor (range: 0-12) and was categorized into three groups (low, moderate, high). LBP was defined as self-reported LBP under treatment. Logistic regression analysis was conducted to calculate the odds ratio (OR) and 95% confidence interval (CI) for LBP.Results: In multivariate logistic regression analysis, the OR for LBP was significantly higher in the moderate risk score group (adjusted OR: 1.33 [95% CI: 1.23-1.44] in men; 1.40 [95% CI: 1.27-1.54] in women) and the high risk score group (adjusted OR: 1.54 [95% CI: 1.43-1.67] in men; 1.83 [95% CI: 1.64-2.03] in women) than in the low risk score group. A trend of higher risk of LBP associated with higher lifestyle risk score was observed in both sexes (p for trend < 0.001). These results were similar even in subgroup analysis by age (20-39, 40-59, and ≥ 60 years) and body mass index (BMI) (< 18.5, 18.5-24.9, and ≥ 25 kg/m2).
Transcriptome sequencing and anthocyanin metabolite analysis determines leaf color of Acer pseudo-sieboldianum in autumn
Authors: Zhao Donghui , Gao Yufu , Rong Liping 
Published: December 2019

Abstract
Background: Acer pseudo-sieboldianum is an often used color-leafed tree native to northeastern China, whose leaves are gorgeous in autumn and is used to great effect in landscaping. Leaf color is an important index affecting the ornamental traits of color-leafed plants. Thus, it is of great significance to study the mechanism in leaf coloring in Acer pseudo-sieboldianum as the development and application prospects of this research are broad, and further the utilization of local characteristics tree species with colored leaves. Results: In this study, the leaves of Acer pseudo-sieboldianum from three stages in the autumn color-changing period were used as materials. In order to investigate the related differentially expressed genes in the process of color transformation, we determined the anthocyanins in leaves and used high-throughput sequencing to analyze the associated transcriptomes. The results demonstrated that five anthocyanins were detected in this experiment. It is worth mentioning that Peonidin O-hexoside, Cyanidin 3-O-glucoside (Kuromanin) and Cyanidin 3,5-O-diglucoside (Cyanin) have significant differences in their content change in two of the tested periods, meaning these may be the key substances determining the final color of Acer pseudo-sieboldianum. A total of 67.47 Gb of clean data were obtained from our sequencing results. Functional annotation of unigenes, including comparison with COG and GO databases, yielded 35,316 unigene annotations. 16521 differentially expressed genes were identified from a statistical analysis of differentially gene expression. We were able to cluster genes related to leaf color formation in Acer pseudo-sieboldianum, including ANS, DFR, F3H, UFGT, and so on, based on the expression at specific times. Conclusions: This study enriched the available transcriptome information for Acer pseudo-sieboldianum and identified a series of differentially expressed genes related to leaf color, which provides valuable information for further study on the genetic mechanism of leaf color expression in Acer pseudo-sieboldianum.
Automatic extraction, prioritization and analysis of gut microbial metabolites from biomedical literature
Authors: Wang Quangqiu , Xu Rong 
Published: December 2019

Abstract
Background: Many diseases are driven by gene-environment interactions. One important environmental factor is the metabolic output of human gut microbiota. A comprehensive catalog of human metabolites originated in microbes is critical for data-driven approaches to understand how microbial metabolism contributes to human health and diseases. Here we present a novel integrated approach to automatically extract and analyze microbial metabolites from 28 million published biomedical records. Results: First, we classified 28,851,232 MEDLINE records into microbial metabolism-related or not. Second, candidate microbial metabolites were extracted from the classified texts. Third, we developed signal prioritization algorithms to further differentiate microbial metabolites from metabolites originated from other resources. Finally, we systematically analyzed the interactions between extracted microbial metabolites and human genes. A total of 11,846 metabolites were extracted from 28 million MEDLINE articles. The combined text classification and signal prioritization significantly enriched true positives among top: manual curation of top 100 metabolites showed a true precision of 0.55, representing a significant 38.3-fold enrichment as compared to the precision of 0.014 for baseline extraction. More importantly, 29% extracted microbial metabolites have not been captured by existing databases. Our data-driven analysis of the interactions between the extracted microbial metabolite and human genetics provides mechanistic insights into how microbiota may contribute to human diseases. Conclusions: This study represents the first effort towards building a comprehensive knowledge base of microbial metabolites, which can set the foundation for future tasks of microbial metabolite relationship extraction from literature and facilitate data-driven studies of how microbial metabolism contributes to human diseases.
The effect of pyridine-alkaloid secondary metabolites on sunbirds gut microbiome
Authors: Gunasekaran Mohanraj , Lalzar Maya , Sharaby Yehonatan , Izhaki Ido , Halpern Malka 
Published: December 2019

Abstract
Background: Sunbirds (Cinnyris osea) feed on nectar of the tobacco tree (Nicotiana glauca), which contains the secondary metabolites nicotine and anabasine. These toxic pyridine-alkaloids presumably serve to deter nectar thieves and allow only specific pollinators to feed on nectar. The floral nectar contains low level of proteins and hence nectarivores may have problem balancing their nitrogen requirements. Our aim was to understand whether the gut microbiome of nectarivores contains bacteria that are able to degrade plant secondary metabolites and how the presence of secondary metabolites in nectar shapes the gut microbiome of nectarivores.Results: Sunbirds (n=16) that were held in captivity were randomly divided into control and treatment groups. The control group was fed with artificial nectar while the treatment group was fed with artificial nectar with the addition of nicotine and anabasine. Excreta were collected from each of the 16 birds on day 0 and at the end of weeks 2, 4 and 7. Nonmetric multidimensional scaling analysis (nMDS) demonstrated that the secondary metabolites significantly changed the gut microbiota composition and diversity of those sunbirds that were fed nectar with added pyridine alkaloids. Aeromonas, Enterobacteriaceae, Exiguobacterium, Klebsiella, Lactococcus, Leuconostoc, Rothia and Salmonella were found to contribute to the differences in gut microbiota between the control and treated birds (birds feed with Nicotine and Anabasine). Four weeks after starting to feed the birds with added nicotine and anabasine, we found that genera with the ability to degrade these pyridine-type metabolites proliferated from a total relative abundance of only 0.3% to 52.7% of the gut bacterial community.Conclusion: Sunbirds feed on Nicotiana nectar that contains toxic pyridine-alkaloids. Nonetheless, the symbiotic gut microbiota which is changing dynamically towards enabling the degradation of these secondary metabolites, supply the birds with nitrogen that enables the synthesis of essential amino acids. The current study contributes to our understanding of the complex relationships between plants, birds and their microbiomes.
miR-373-3p inhibits EMT via regulation of TGFβR2 in choriocarcinoma
Authors: lu yanjie , Li Xiaoru , Zuo Yanzhen , xu qian , liu lei , wu haiying , chen long , zhang ying , fan wenjing , liu ying , li yuhong 
Published: December 2019

Abstract
Background: Previous studies have indicated that early metastasis is a major cause of mortality in patients with choriocarcinoma. However, what determines whether early metastasis of choriocarcinoma has occurred is unknown. The emerging role of miRNA in regulating cancer development and progression has been recognized. MiR-373-3p has been shown to play pivotal roles in tumorigenesis and metastasis. However, whether miR-373-3p functions to promote choriocarcinoma metastasis is not clear. The purpose of this study is to determine the function of miR-373-3p in the progression of choriocarcinoma. Methods: In this study, we first compared EMT-related markers, which are inversely correlated with miR-373-3p expression, in trophoblast and choriocarcinoma cell lines. Using PCR and western blot, the upregulation of miR‑373‑3p was observed to inhibit EMT progression. Similarly, gain-and loss-of-function studies revealed that ectopic miR-373-3p overexpression inhibited the metastasis of choriocarcinoma cells. Results: Our results revealed that miR-373-3p functions as an inhibitor in JEG-3 and JAR cells; this is due to its mediation of the TGF-β signalling pathway, which is responsible for EMT. The bioinformatic analysis and dual‑luciferase reporter gene assays were employed to verify that miR‑373‑3p might interact with the 3' untranslated region of TGFβR2 mRNA. Further western blot results showed miR‑373‑3 preversed the increases of TGFβR2 and inhibited EMT. Conclusions: In light of our observations, miR‑373‑3p upregulation partly accounts for TGFβR2 downregulation and leads to a restraint of EMT and metastasis. MiR‑373‑3p may, therefore, serve as a valuable target in potential anticancer strategies to treat choriocarcinoma.
The utility of six serum tumor markers in early detection of lung cancer
Authors: Jiang Qingwen , Zheng Xu , Li Yiting , Huang Weina , Li Xinjian , Cao Chao 
Published: December 2019

Abstract
Background: Lung cancer has become the leading cause of cancer-related death in China. However, most of patients were diagnosed at advanced stage. Thus, novel lung cancer diagnostic tests, which can be used to screen individuals in early stage, are required.Methods: A total of 208 patients involving 161 cases of lung cancer and 47 cases of benign diseases were enrolled. Serum concentration of GTM, CETM, PTM, CTM, ETM and HTM were analyzed by kits according to the manufacturer’s guidelines.Results: The results showed significant difference in serum concentrations of GTM, CETM, PTM, CTM, ETM, and HTM between patients with lung cancer and benign diseases. In addition, when compared with benign diseases, higher levels of those six markers were also observed in patient with SCC and SCLC, but not for ADC. Receiver operating characteristic (ROC) curves were further suggested a high sensitivity and specificity of six markers to identify lung cancer.Conclusion: The serum levels of GTM, CETM, PTM, CTM, ETM and HTM in lung cancer were significantly higher than those of benign diseases. Moreover, these six biomarkers showed a high sensitivity and specificity to identify a patient with malignant. These findings suggested that detection of those six biomarkers in serum might be helpful for differential diagnosis of lung cancer.
Expression of selected MicroRNAs in pancreatic ductal adenocarcinoma in relation to tumor progression and patient’s outcome.
Authors: Popov Alexey , Szabo Arpad , Gurlich Robert , Liberko Marian , Soumarova Renata , Vernerova Zdenka , Mandys Vaclav 
Published: December 2019

Abstract
Background: pancreatic ductal adenocarcinoma (PDAC) remains a disease with extremely poor prognosis and limited effective available treatment. Differential expression of miRNAs isolated from tumor tissue has been proposed as a marker for tumor diagnosis, progression and prognosis. Nevertheless, prognostic value of miRNAs expression in PDACs for patient outcome still remains unclear.Methods: expression of 7 selected miRNAs, isolated from FFPE samples of 54 PDAC patients, was quantified using RT-qPCR. The relationship of miRNA expression levels with tumor histology, clinico-pathological characteristics, patient overall survival (OS) and progress-free survival (PFS), was subsequently evaluated.Results: overexpression of miR-21, miR-155 and miR-210 was observed in PDACs (up to 72.62-fold, 232.36 and 181.38-fold respectively), in comparison with non-neoplastic tissues. On the other hand, miR-96 and miR-217 were significantly downregulated in PDACs (up to one hundred times). No differences were, however, noticed between cancer and normal tissues for the expression levels of miR-148a and miR-196a.On the other hand, expression levels of all 7 miRNAs failed to demonstrate significant correlation with parameters of tumor progression, such as tumor stage, grade, nodal involvement, perineural and vascular invasion. Positive correlation of miR-210 levels was, however, observed with patient age (ρ=0.35). Additionally, miR-148a and miR-217 expression have shown positive association with tubular tumor growth pattern (ρ=0.39; ρ=0.28). Negative correlation of miR-148a values was also demonstrated with dissociative growth pattern and nuclear atypia (ρ=-0.30; ρ=0.27).
ANÁLISE MULTIVARIADA DE TEMPO, TEMPERATURA E AGITAÇÃO NA TRANSFERÊNCIA DE MASSA DO CLORETO DE SÓDIO EM ÁGUA
Authors: Aguiar Polyana Gomes de , Fernandes João Paulo Silva , Magalhães Mariana Cordeiro , Dias Daiane Ribeiro , Andrade Isabelle Santos , Gonçalves João Carlos , Cardoso Thalles de Assis Gonçalves 
Published: December 2019

O transporte difusivo e convectivo são essenciais para a transferência de massa nas reações químicas e a sua eficácia pode variar conforme alguns fatores relevantes nas construções de unidades industriais. As variáveis de um sistema podem influenciar diretamente no processo, sendo assim, tornam-se válidas as suas análises, a fim de se encontrar condição ótima de operação. Feita essa consideração, no presente trabalho realizou-se o planejamento fatorial de preparo da solução de cloreto de sódio com intuito de realizar tratamento estatístico dos resultados obtidos para condutividade do sistema quando a agitação, temperatura e tempo assumiram valores em dois níveis de estudo. A partir da realização de 8 (oito) experimentos em triplicata foi possível obter a média de condutividade para cada ensaio e, assim, gerar o Gráfico de Pareto, Modelo de regressão múltipla, teste de significância da regressão, Superfícies de Resposta e Parâmetros F e p. Observou-se que a variável temperatura e a sua combinação com a agitação podem ser consideradas significativas na transferência de massa e, devido ao fato das suas interferências serem lineares, valores máximos dessas propriedades podem ser considerados pontos ótimos. No caso dessa aplicação, o ponto ótimo se deu em temperatura de 60°C.