bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined effects of maternal exposure to fungicides on behavioral development in F1‐generation mice: 2. Fixed‐dose study of thiabendazole
Authors: Tanaka Toyohito , Suzuki Toshinari , Inomata Akiko , Moriyasu Takako 
Published: September 2020

AbstractBackgroundFew published studies are reported for the neurobehavioral toxicity of combined exposure to fungicides in mammals. This study was aimed to re‐evaluate the reproductive and neurobehavioral effects of maternal exposure to combined imazalil (IMZ) and thiabendazole (TBZ) with fixed‐dose of TBZ in mice.MethodsIMZ/TBZ were given in the diet to provide levels of 0%/0% (control), 0.0015%/0.018% (IMZ/TBZ), 0.006%/0.018% and 0.024%/0.018% during the gestation and lactation periods. Selected reproductive and neurobehavioral parameters were measured in the F1 generation.ResultsNo adverse effect of IMZ/TBZ was observed in litter size, litter weight, or sex ratio at birth. Concerning behavioral developmental parameters, the cliff avoidance on PND 7 of male offspring was restrained significantly in the treatment groups in a dose‐related manner. Exploratory behavior examination indicated that the average time of rearing significantly lengthened in the high‐dose group of male offspring. After weaning, the average time of rearing in exploratory behavior lengthened in a significant dose‐related trend in adult females of the F1 generation. Spontaneous behavior examination indicated that the average speed decreased significantly through 120 min in the high‐dose group of the F1‐generation males. In females, the average time of rearing lengthened significantly through 120 min in the high‐dose group. In the longitudinal patterns, the parallel lines of the control and treatment groups indicated a significant distance in the average time of rearing in the F1‐generation females.ConclusionsThe results from two combined exposure studies of IMZ/TBZ suggest that TBZ concentrations have caused major effects on exploratory and spontaneous behavior.
Benefits of recreational dance and behavior analysis for individuals with neurodevelopmental disorders: A literature review
Authors: Pontone Madeline , Vause Tricia , Zonneveld Kimberley L. M. 
Published: September 2020

AbstractDance can be an entertaining experience that offers multiple benefits for those who participate. Unlike typically developing populations, studies examining benefits of recreational dance for individuals with neurodevelopmental disorders are limited. In this review, we conducted a literature search, where “dance” was cross‐listed with all neurodevelopmental disorders across five databases, yielding 19 articles. Twelve studies involved children and youth with neurodevelopmental disorders aged 3–19 years, two studies included both adolescents and adults aged 14–22 years, and five studies included adults aged 20–65 years. Given the effectiveness of applied behavior analysis in enhancing skill development, it is encouraging that eight studies explicitly identified behavioral components. Results suggest potential benefits of recreational dance across studies using self‐report and objective measures, but limited research with sound methodology exists. There is a need for controlled research with measurable outcomes to evaluate programs tailored to these populations to improve core challenges and secondary outcomes such as quality of life.
Quercetin and hydroxytyrosol as modulators of hepatic steatosis: A NAFLD‐on‐a‐chip study
Authors: Gori Manuele , Giannitelli Sara Maria , Zancla Andrea , Mozetic Pamela , Trombetta Marcella , Merendino Nicolò , Rainer Alberto 
Published: September 2020

AbstractOrgans‐on‐chip (OoCs) are catching on as a promising and valuable alternative to animal models, in line with the 3Rs initiative. OoCs enable the creation of three‐dimensional (3D) tissue microenvironments with physiological and pathological relevance at unparalleled precision and complexity, offering new opportunities to model human diseases and to test the potential therapeutic effect of drugs, while overcoming the limited predictive accuracy of conventional 2D culture systems. Here, we present a liver‐on‐a‐chip model to investigate the effects of two naturally occurring polyphenols, namely quercetin and hydroxytyrosol, on nonalcoholic fatty liver disease (NAFLD) using a high‐content analysis readout methodology. NAFLD is currently the most common form of chronic liver disease; however, its complex pathogenesis is still far from being elucidated, and no definitive treatment has been established so far. In our experiments, we observed that both polyphenols seem to restrain the progression of the free fatty acid‐induced hepatocellular steatosis, showing a cytoprotective effect due to their antioxidant and lipid‐lowering properties. In conclusion, the findings of the present work could guide novel strategies to contrast the onset and progression of NAFLD.
Investigating the science attitudes of students from low socioeconomic status families: The impact of problem‐based learning
Authors: Seçgin Tuba , Sungur Semra 
Published: September 2020

AbstractThis study aimed to examine the effect of the problem‐based learning (PBL) on science attitudes of middle school students from low socio‐economic status families. For this purpose, two intact classes of the same teacher were randomly assigned as experimental and control groups. In the unit of genetics, the experimental group was taught by PBL while the control group received traditional instruction. Students' attitudes were measured in terms of adoption of scientific attitudes, enjoyment of science lessons, and career interest in science administering a self‐report instrument as a pre‐test and a post‐test. Results showed that after the treatment, although there were not statistically significant differences between two groups with respect to attitude toward science; in the experimental group, the mean scores were found to be consistently higher across all attitude dimensions compared to the control group. In addition, the mean scores suggested an improvement in the attitudes of experimental group students from pre‐test to post test. Qualitative findings also revealed that students found PBL enjoyable. According to PBL students, searching for information and writing reports were among the features of the PBL contributing to their learning the most.
From blood to milk: Graphical systems modeling in secondary‐level biochemistry courses
Authors: Gomez Noe A. , Robinson Peter H. 
Published: September 2020

AbstractGiven the present need for biochemistry and molecular biology educators to transform their courses into an online format, novel methods aimed at promoting student learning and engagement must be considered. Herein, we describe the integration of graphical systems modeling as a tool for introducing biochemistry to secondary‐level students. We propose the use of graphic technologies as a way for students to create systems models that describe phenomena of life, such as lactation. Through these endeavors, educators can provide a virtual format for students to continue learning and completing assignments.
A class of low‐cost alternatives to kifunensine for increasing high mannose N‐linked glycosylation for monoclonal antibody production in Chinese hamster ovary cells
Authors: Brantley Timothy J. , Mitchelson Fernie G. , Khattak Sarwat F. 
Published: September 2020

AbstractN‐linked glycosylation of therapeutic monoclonal antibodies is an important product quality attribute for drug safety and efficacy. An increase in the percent of high mannose N‐linked glycosylation may be required for drug efficacy or to match the glycosylation profile of the innovator drug during the development of a biosimilar. In this study, the addition of several chemical additives to a cell culture process resulted in high mannose N‐glycans on monoclonal antibodies produced by Chinese hamster ovary (CHO) cells without impacting cell culture performance. The additives, which include known mannosidase inhibitors (kifunensine and deoxymannojirimycin) as well as novel inhibitors (tris, bis–tris, and 1‐amino‐1‐methyl‐1,3‐propanediol), contain one similar molecular structure: 2‐amino‐1,3‐propanediol, commonly referred to as serinol. The shared chemical structure provides insight into the binding and inhibition of mannosidase in CHO cells. One of the novel inhibitors, tris, is safer compared to kifunensine, 35x as cost‐effective, and stable at room temperature. In addition, tris and bis–tris provide multiple low‐cost alternatives to kifunensine for manipulating glycosylation in monoclonal antibody production in a cell culture process with minimal impact to productivity or cell health.
A tale of two systems: The tracts of my tears
Authors: Neumann Paul E. 
Published: September 2020

AbstractMost of the terms in Terminologia Anatomica are arranged by organ systems (systemata); however, the names for these systems are often used by physiologists and other biomedical scientists for related functional systems. These functional systems may differ in their composition from the anatomist's organ systems. In addition, many functional systems have been described that do not correspond to organ systems. Similarly, the non‐standard anatomical terms alimentary tract, respiratory tract, urinary tract and reproductive tract lack a consistent meaning in the biomedical sciences. For clear communication, one should be cautious when using terms that may have other meanings in other disciplines. This appears to be particularly true for high level terms in the nomenclature of systematic anatomy.
3D morphological classification of the nasolacrimal duct: Anatomical study for planning treatment of tear drainage obstruction
Authors: Lee Sohyun , Lee U‐Young , Yang Suk‐Woo , Lee Won‐Joon , Kim Dong‐Ho , Youn Kwan Hyun , Kim Yi‐Suk 
Published: September 2020

AbstractBackgroundThe purpose of this study is to analyze and classify morphological features of the nasolacrimal duct (NLD) through 3D reconstruction to help understand the causes and treatment of NLD obstruction.MethodsIn this study, we included 63 males and 55 females who underwent autopsy without NLD obstruction with ages ranging from 20 to 78 years. The NLD was defined from the lacrimal fossa to the opening of the BNLD to the inferior meatus, and all continuous CT images showing the NLD were selected. Segmentation was performed semi‐automatically, and the reconstruction and measurement of NLD was performed using the Mimics program.ResultsOverall NLD length, bony nasolacrimal duct (BNLD) length, anteroposterior and transverse diameters at the entrance to the BNLD, anteroposterior and transverse smallest diameters of the BNLD, BNLD volume, and lacrimal sac BNLD angle were significantly higher in males than females (p < .05). BNLD direction in the coronal plane was slightly more likely to be inward. The most common type in both sexes was cylinder type (42.0%), males were more likely to have lower‐thicker types (34.1%), and females more likely to have upper‐thicker types (22.7%).ConclusionThere were sex differences in NLD measurements, and females had significantly smaller NLDs. These results may partially explain the increased prevalence of primary acquired NLD obstruction in females. The BNLD tends toward the midline, and inclines posteriorly.
Proteomics identifies EGF‐like domain multiple 7 as a potential therapeutic target for epidermal growth factor receptor‐positive glioma
Authors: Wang Fei‐yi‐fan , Wang‐gou Si‐yi , Cao Hang , Jiang Nian , Yang Qi , Huang Qi , Huang Chun‐hai , Li Xue‐jun 
Published: September 2020

AbstractBackgroundGlioma, the most frequent primary tumor of the central nervous system, has poor prognosis. The epidermal growth factor receptor (EGFR) pathway and angiogenesis play important roles in glioma growth, invasion, and recurrence. The present study aimed to use proteomic methods to probe into the role of the EGF‐EGFR‐angiogenesis axis in the tumorigenesis of glioma and access the therapeutic efficacy of selumetinib on glioma.MethodsProteomic profiling was used to characterize 200 paired EGFR‐positive and EGFR‐negative glioma tissues of all pathological types. The quantitative mass spectrometry data were used for systematic analysis of the proteomic profiles of 10 EGFR‐positive and 10 EGFR‐negative glioma cases. Consensus‐clustering analysis was used to screen target proteins. Immunofluorescence analysis, cell growth assay, and intracranial xenograft experiments were used to verify and test the therapeutic effect of selumetinib on glioma.ResultsAdvanced proteomic screening demonstrated that the expression of EGF‐like domain multiple 7 (EGFL7) was higher in EGFR‐positive tumor tissues than in EGFR‐negative tumor tissues. In addition, EGFL7 could act as an activator in vitro and in vivo to promote glioma cell proliferation. EGFL7 was associated strongly with EGFR and prognosis. EGFL7 knockdown effectively suppressed glioma cell proliferation. Selumetinib treatment showed tumor reduction effect in EGFR‐positive glioblastoma xenograft mouse model.ConclusionsEGFL7 is a potential diagnostic biomarker and therapeutic target of glioma. Selumetinib could target the EGFR pathway and possibly improve the prognosis of EGFR‐positive glioma.
Stimulatory Effects ofPaederia foetidaFlower Absolute on the Skin Wound and Barrier Repair Activities of Keratinocytes
Authors: Kim Ha Bin , Won Kyung Jong , Kim Do Yoon , Kang Hye Min , Lee Hwan Myung 
Published: September 2020

AbstractPaederia foetida(PF) has antidiarrheal, antidiabetic, and anti‐inflammatory activities. However, its biological activities on skin remain unclear. In this study, we examined the effect of PF flower absolute (PFFA) on skin wound healing‐ and skin barrier‐linked responses in human epidermal keratinocytes (HaCaT cells). PFFA contained 23 components and increased the proliferation and sprout outgrowth of HaCaT cells and modestly increased migration. PFFA enhanced the phosphorylation levels of extracellular signal‐regulated kinase1/2, serine/threonine‐specific protein kinase (AKT), and p38 mitogen‐activated protein kinase (MAPK) in HaCaT cells, and upregulated type I and IV collagen synthesis and filaggrin (an epidermal barrier protein) expression in HaCaT cells. These findings suggest PFFA may promote skin wound repair by stimulating migratory and proliferative activities (probably through the AKT/MAPK pathway), collagen synthesis, and skin barrier repair by upregulating the expressions of filaggrin in epidermal keratinocytes. Therefore, PFFA may be useful for developing agents that enhance skin wound and barrier‐repair functions.
Pyrrolidinyl Peptide Nucleic Acid Probes Capable of Crosslinking with DNA: Effects of Terminal and Internal Modifications on Crosslink Efficiency
Authors: Muangkaew Penthip , Vilaivan Tirayut 
Published: September 2020

AbstractIn this study, we describe a furan‐modified acpcPNA as a probe that can form an interstrand crosslink (ICL) with its DNA target upon activation with N‐bromosuccinimide (NBS). To overcome the problem of furan instability under acidic conditions, a simple and versatile post‐synthetic methodology for the attachment of the furan group to the PNA probe was developed. Unlike in other designs, the furan was placed at the end of the PNA molecule or tethered to the PNA backbone with all the base pairs in the PNA ⋅ DNA duplexes fully preserved. Hence, the true reactivity of each nucleobase towards the crosslinking could be compared. We show that all DNA bases except T could participate in the crosslinking reaction when the furan was placed at the end of the PNA strand. The crosslinking process was sensitive to mispairing, and lower crosslinking efficiency was observed in the presence of a base‐mismatch in the PNA ⋅ DNA duplex. In contrast, when the furan was placed at internal positions of the acpcPNA ⋅ DNA duplex, no ICL was observed; this was explained by the inability of a hydrogen‐bonded nucleobase to participate in the crosslinking reaction. The crosslinking efficiency was considerably improved, despite lower duplex stability, when an unpaired base (in the form of C‐insertion) was present in the complementary DNA strand close to the furan modification site.
Trends of repeat revascularization choice in patients with prior coronary artery bypass surgery
Authors: Mohamed Mohamed O. , Shoaib Ahmad , Gogas Bill , Patel Tejas , Alraies M Chadi , Velagapudi Poonam , Chugh Sanjay , Sharma Kamal , Mohamed Walid , Murphy Gavin J. , Kwok Chun Shing , Rashid Muhammad , Bagur Rodrigo , Mamas Mamas A. 
Published: September 2020

AbstractObjectiveTo examine rates and predictors repeat revascularization strategies (percutaneous coronary intervention [PCI] and coronary artery bypass grafting [CABG]) in patients with prior CABG.MethodsUsing the National Inpatient Sample, patients with a history of CABG hospitalized for revascularization by PCI or CABG from January 2004 to September 2015 were included. Regression analyses were performed to examine predictors of receipt of either revascularization strategy as well as in‐hospital outcomes.ResultsThe rate of redo CABG doubled between 2004 (5.3%) and 2015 (10.3%). Patients who underwent redo CABG were more comorbid and experienced significantly worse major adverse cardiovascular and cerebrovascular events (odds ratio [OR]: 5.36 95% CI 5.11–5.61), mortality (OR 2.84 95% CI 2.60,−3.11), bleeding (OR 5.97 95% CI 5.44–6.55) and stroke (OR 2.15 95% CI 1.92–2.41), but there was no difference in cardiac complications between groups. Thoracic complications were high in patients undergoing redo CABG (8%), especially in females. Factors favoring receipt of redo CABG compared to PCI included male sex, age < 80 years, and absence of diabetes and renal failure.ConclusionReoperation in patients with prior CABG has doubled in the United States over a 12‐year period. Patients undergoing redo CABG are more complex and associated with worse clinical outcomes than those receiving PCI.
Carotid access for transcatheter aortic valve replacement: A meta‐analysis
Authors: Sharma Sharan Prakash , Chaudhary Rahul , Ghuneim Angela , Harder William , David Shukri , Choksi Nishit , Kondur Snigdha , Kambhatla Swathi , Kambhatla Sujata , Kondur Ashok 
Published: September 2020

AbstractObjectiveWe sought to evaluate the feasibility and safety of carotid access transcatheter aortic valve replacement (TAVR) by performing a meta‐analysis of published cases.BackgroundSeveral case series and regional data have provided initial basis for carotid access TAVR in patients with prohibitive femoral approach. We performed this meta‐analysis to provide further evidence of feasibility and safety of carotid TAVR.MethodsWe searched PubMed, EMBASE, CINAHL, and Cochrane CENTRAL for any study on carotid access TAVR involving ⩾5 patients since inception till March 1, 2020. Random‐effects model was used to compute overall effects. The outcomes analyzed were all‐cause mortality, Transient ischemic attack (TIA)/stroke, need for permanent pacemaker (PPM) implantation, pericardial tamponade, access site complications, major bleeding, and length of stay.ResultsThere was a total of 17 retrospective studies (n = 2082) with a median follow‐up of 1 month. Mean age of the patient was 80 years. Mean Euroscore and STS scores were 15 ± 6.2 and 7.9 ± 3.3, respectively. The procedural success rate was 99%. The rate of all‐cause mortality was 6.7% (range 4.6–9.7%, p < .001, I2 = 67%). Incidence of TIA/stroke was 3.9% (range 3.1–4.8%, p < .001, I2 = 0%) and PPM implantation was 16.7% (range 12.5–21.9%, p < .001, I2 = 56%). Rate of pericardial tamponade, vascular complication, and major bleeding were 1.7, 2.5, and 7%, respectively. Average length of hospital stay was 7.7 days.ConclusionOur results show that transcarotid approach is a feasible option in patients with prohibitive femoral access for TAVR.
Individual patient data analysis of the BIOFLOW study program comparing safety and efficacy of a bioresorbable polymer sirolimus eluting stent to a durable polymer everolimus eluting stent
Authors: Toelg Ralph , Slagboom Ton , Waltenberger Johannes , Lefèvre Thierry , Saito Shigeru , Kandzari David E. , Koolen Jacques , Richardt Gert 
Published: September 2020

AbstractObjectivesThis analysis of pooled individual patient data (IPD) aimed to evaluate the safety and efficacy of a bioresorbable polymer sirolimus eluting stent system (BP‐SES; Orsiro) compared to a durable polymer everolimus eluting stent system (DP‐EES; Xience) in the pooled population as well as in subgroups.MethodsIPD with up to 12 months follow‐up of the randomized controlled trials BIOFLOW‐II (NCT01356888), ‐IV (NCT01939249), and ‐V (NCT02389946) as well as the all comers registry BIOFLOW‐III (NCT01553526) were pooled. A total of 3,717 subjects (2,923 in BP‐SES and 794 in DP‐EES) with 5,328 lesions (4,225 lesions in BP‐SES and 1,103 in DP‐EES) were included in the IPD. The primary endpoint was target lesion failure (TLF) at 12 months follow‐up. Subgroups analyzed included diabetes, age (≥65 years), gender, complex lesions (B2/C), small vessels (reference vessel diameter ≤2.75 mm), multivessel treatment, renal disease, and patients with acute coronary syndrome.ResultsOverall, TLF at 12 months was significantly lower with 5.2%in the BP‐SES group versus 7.6% in the DP‐EES group (p = .0098). Similarly, target vessel myocardial infarction (TV‐MI) was 3.1 versus 5.7% (p = .0005). The rate of stent thrombosis was similar in both groups (0.004%). By regression analysis, an independent stent effect in favor of BP‐SES was observed for TLF (p = .0043) and TV‐MI (p = .0364) in small vessels.ConclusionResults of this IPD analysis suggest that the BP‐SES with ultrathin struts is as safe as and more efficacious than DP‐EES in the overall cohort and especially in small vessels.
A Novel Visible‐Light‐Responsive Semiconductor ScTaO4−xNx for Photocatalytic Oxygen and Hydrogen Evolution Reactions
Authors: Pei Lang , Cai Hong , Jin Hao , Li Taozhu , Zhu Heng , Yuan Yongjun , Zhong Jiasong , Yan Shicheng , Zou Zhigang 
Published: September 2020

AbstractScreening of visible‐light‐responsive semiconductor photocatalysts is a significant target for solar water splitting systems. Herein, a newly designed Ta‐based oxynitride, namely ScTaO4−xNx, was successfully synthesized via nitrogen doping of ScTaO4 oxide precursors at 1273 K under NH3 flow. The ScTaO4−xNx photocatalyst, with a strong band‐to‐band visible light absorption as far as 540 nm, can be employed for both water oxidation and reduction half‐reactions under visible light illumination, demonstrating great potential for photocatalytic water splitting.
Sinterability of macrocrystalline and cryptocrystalline magnesite to refractory magnesia
Authors: Guo Zongqi , Ma Ying , Rigaud Michel 
Published: September 2020

AbstractThe purpose of this paper is to present the results of an investigation aiming at obtaining high quality sintered magnesia from two well‐known Chinese magnesite. Two types of natural magnesite have been considered, one macrocrystalline, from Liaoning, with the grain size of 60‐100 µm and one cryptocrystalline, from Tibet, with 2‐4 µm grains. Calcining characteristics to transform the two magnesite to caustic magnesia have been studied at first. Subsequently, the calcining‐sintering characteristics using a two‐ and a three‐step process for both raw materials at different temperatures have been determined. A DTA–TGA study reveals that the minimum endothermic peak during the calcining step differs by 28°C (624 vs 652°C between the crypto‐ and macrocrystalline magnesite). It has also been observed that by using a two‐step sintering process, it is possible to obtain a magnesia with a bulk density of 3.26 and 3.14 g/cm3, respectively, for cryptocrystalline and macrocrystalline, while with a three‐step process, it is possible to reach a bulk density of 3.48 g/cm3, even at a temperature <1750°C.