bluuebunny/crossref_metadata_2025_split huggingface dataset:
Obstetrical and intensive care strategies in a high-risk pregnancy with critical respiratory failure due to COVID-19: a case report
Authors: Kolkova Zuzana , Bjurstrom Martin , Lansberg John-Kalle , Svedas Eimantas , Andrada Maria , Hansson Stefan , Herbst Andreas , Zaigham Mehreen 
Published: July 2020

Abstract
BackgroundWith the disease burden increasing daily, there is a lack of evidence regarding the impact of COVID-19 in pregnancy. Healthy pregnant women are still not regarded as a susceptible group despite physiological changes that make pregnant women more vulnerable to severe infection. However, high-risk pregnancies may be associated with severe COVID-19 disease with respiratory failure, as outlined in this report. We discuss the importance of timely delivery and antenatal steroid administration in a critically ill patient.CaseA 27-year-old pregnant woman (1-para) with type I diabetes, morbid obesity, hypothyroidism and a previous Caesarean section, presented with critical respiratory failure secondary to COVID-19 at 32 weeks of gestation. A preterm emergency Caesarean section was performed, after steroid treatment for foetal lung maturation. The patient benefited from prone positioning however, transient acute renal injury, rhabdomyolysis and sepsis led to prolonged intensive care and mechanical ventilation for 26 days post-Caesarean. The baby had an uncomplicated recovery.ConclusionCOVID-19 infection in high-risk pregnancies may result in severe maternal-neonatal outcomes such as critical respiratory failure requiring mechanical ventilation and premature termination of the pregnancy. Antenatal steroids may be of benefit for foetal lung maturation but should not delay delivery in severe cases.
Efficient Masked Face Recognition Method during the COVID-19 Pandemic
Authors: Hariri Walid 
Published: July 2020

Abstract
The COVID-19 is an unparalleled crisis leading to huge number of casualties and security problems. In order to reduce the spread of coronavirus, people often wear masks to protect themselves. This makes the face recognition a very difficult task since certain parts of the face are hidden. A primary focus of researchers during the ongoing coronavirus pandemic is to come up with suggestions to handle this problem through rapid and efficient solutions. In this paper, we propose a reliable method based on discard masked region and deep learning based features in order to address the problem of masked face recognition process. The first step is to discard the masked face region. Next, we apply a pre-trained deep Convolutional neural networks (CNN) to extract the best features from the obtained regions (mostly eyes and forehead regions). Finally, the Bag-of-features paradigm is applied on the feature maps of the last convolutional layer in order to quantize them and to get a slight representation comparing to the fully connected layer of classical CNN. Finally, MLP is applied for the classification process. Experimental results on Real-World-Masked-Face-Dataset show high recognition performance.
Neuroprotective Effect of Angelica Gigas Root in a Mouse Model of Ischemic Brain Injury through MAPK Signaling Pathway Regulation
Authors: Lee Se-Eun , Kim Jung-Hoon , Lim Chiyeon , Cho Suin 
Published: July 2020

Abstract
Background: The root of Angelica gigas Nakai (Apiaceae) has been traditionally used as an important herbal medicine to treat blood-deficiency-related disorders in Eastern Asian countries, and recently, it has been recognized as a potential candidate for improving cardiovascular diseases. Methods: In this study, the neuroprotective effect of a methanol extract of A. gigas root (RAGE) was investigated in a mouse stroke model induced by a 90 min transient middle cerebral artery occlusion (tMCAO). Infarction volumes and morphological changes in brain tissues were measured using TTC, cresyl violet, and H&E staining. The neuroprotective mechanism of RAGE was elucidated through investigation of protein expression levels using western blotting, IHC, and ELISA assays. The plasma concentrations of decursin, a major compound in RAGE, were measured after oral administration of RAGE to SD rats. Results: The infarction volumes in brain tissues were significantly reduced and the morphological deteriorations in the brain neuron cells were improved in tMCAO mice when pre-treated with RAGE at 1,000 mg/(kg bw·d) for two consecutive days. The neuroprotective mechanism of RAGE was confirmed to attenuate ERK-related MAPK signaling pathways in the ipsilateral hippocampus hemisphere in mice. The concentrations of decursin in rat plasma samples showed peak absorption and elimination in vivo after oral administration of RAGE at 100 mg/rat.Conclusion: Mice administered RAGE before the tMCAO operation had less neuronal cell death than those that were not administered RAGE prior to the operation, and this study provides preclinical evidence for use of A. gigas in ischemic stroke.
m5CPred-SVM: A Novel Method for Predicting m5C Sites of RNA
Authors: Chen Xiao , Xiong Yi , Liu Yinbo , Chen Yuqing , Bi Shoudong , Zhu Xiaolei 
Published: July 2020

Abstract
Background: As one of the most common post-transcriptional modifications (PTCM) in RNA, 5-cytosine-methylation plays important roles in many biological functionssuch as RNA metabolism and cell fate decision. Through accurate identification of 5-methylcytosine (m5C) sites on RNA,researcherscanbetter understandthe exact role of 5-cytosine-methylation in these biological functions. In recent years, computational methods of predicting m5C sites have attracted lots of interests because of its efficiency and low-cost.However, both the accuracy and efficiency of these methods are not satisfactory yet and need further improvement.Results: In this work, we have developed a new computational method, m5CPred-SVM, to identify m5C sites in three species, H. sapiens, M. musculus and A. thaliana. To build this model, we first collected benchmark datasets following three recently published methods. Then, six types of sequence-based features were generated based on RNA segments and the sequential forward feature selection strategy was used to obtain the optimal feature subset. After that, the performance of models based on different learning algorithms were compared, and the model based on the support vector machine provided the highest prediction accuracy. Finally, our proposed method, m5CPred-SVM was compared with several existing methods, and the result showed that m5CPred-SVMoffered substantially higher prediction accuracy thanpreviously published methods. It is expected that our method, m5CPred-SVM, can become a useful tool for accurate identification of m5C sites.Conclusion: In this study, by introducing position-specific propensity related features, we built a new model, m5CPred-SVM, to predict RNA m5C sites of three different species.The result shows that our model outperformed the existing state-of-art models.Our model is available for users through a web serverat http://zhulab.ahu.edu.cn/m5CPred-SVM.
Successful Refractory of Acute Myocardial Infarction-induced Electrical Storm by Defibrillations and Amiodarone: A Case Report
Authors: Guo Huanhuan , Gan Quan 
Published: July 2020

Abstract
Background: Acute myocardial infarction (AMI) often complicated with multiple arrhythmias, especially ventricular arrhythmias, including sustained ventricular tachycardia (SVT) and ventricular fibrillation (VF) are often presages progressive heart failure in 48 hours. The present study reports a case of electrical storm (ES) occurring in an 84-year-old woman with acute myocardial infarction (AMI).Case presentation: With the defibrillations or amiodarone, the recurrence of ventricular tachycardia inhibited and the electrocardiographic pattern normalized.Conclusions: The results suggest that defibrillations and amiodarone may be able to improve the survival rate of patients with ES with AMI and would be considered as an alternative treatment for implantable cardioverter defibrillator (ICD) and invasive catheter ablation in the management of cardiac ES.
Exclusive Peripheral Overexpression of 5-HT5A Receptors is Involved in 5‑HT‑Induced Cardiac Sympatho-Inhibition in Type 1 Diabetic Rats
Authors: Garcia-Pedraza Jose Ángel , Hernández-Abreu Oswaldo , Morán Asunción , Carretero José , García-Domingo Mónica , Villalón Carlos M 
Published: July 2020

Abstract
Background: In normoglycaemic pithed rats, cardiac sympathetic control is modulated by serotonin (5-hydroxytryptamine; 5‑HT), which inhibits the cardioaccelerator sympathetic outflow via the activation of 5-HT 1B , 5-HT 1D and 5-HT 5A receptors. Notwithstanding, type 1 diabetes impairs the functionality of the cardiac sympathetic innervation and leads to cardiovascular complications including cardiac autonomic neuropathy. On this basis, the present study investigated whether the influence of 5-HT on cardiac noradrenergic neurotransmission is altered in type 1 diabetic rats, by analysing the profile of the 5-HT receptors involved and their peripheral expression.

Methods: Type 1 diabetes was induced in male Wistar rats with a single injection of streptozotocin (50 mg/kg, i.p.). Four weeks later, the rats were anaesthetized, pithed and prepared for producing tachycardic responses by either electrical preganglionic stimulation (C 7 ‑T 1 ) of the cardioaccelerator sympathetic outflow or i.v. bolus injections of exogenous noradrenaline. Immunohistochemistry analyses were performed to study the expression of 5‑HT 1B , 5-HT 1D and 5-HT 5A receptors in the stellate (sympathetic) ganglion obtained from normoglycaemic and diabetic rats.

Results: The increases in heart rate evoked by both cardiac sympathetic stimulation and exogenous noradrenaline were not modified after saline in diabetic rats. Moreover, i.v. continuous infusions of 5‑HT induced a cardiac sympatho-inhibition that was mimicked by the 5‑HT 1/5A receptor agonist 5‑carboxamidotryptamine, but not by the agonists indorenate (5-HT 1A ), CP 93,129 (5‑HT 1B ), PNU 142633 (5-HT 1D ), or LY344864 (5‑HT 1F ) in the diabetic group. In contrast, the above agonists at 5-HT 1B , 5-HT 1D and 5-HT 1/5A receptors mimicked 5-HT-induced sympatho-inhibition in normoglycaemic rats. In diabetic animals, i.v. administration of SB 699551 (1 mg/kg; 5‑HT 5A receptor antagonist) abolished 5‑CT-induced cardiac sympatho-inhibition. Finally, the immunohistochemistry analysis in the stellate ganglion showed that, as compared to normoglycaemic rats, in diabetic rats (P<0.05): (i) the expression of 5-HT 1B receptors was slightly higher, whereas that of 5-HT 1D receptors was slightly lower; and (ii) there was a clear overexpression of 5-HT 5A receptors.

Conclusions: Taken together, these results show the prominent role of the peripheral overexpression of prejunctional 5-HT 5A receptors in the inhibition of the cardiac sympathetic drive in type 1 diabetic rats. These findings may represent a new pharmacological strategy for the treatment of diabetes-related cardiac abnormalities.
The Influence of Preoperative Psychological States on Postoperative Outcomes Following Percutaneous Kyphoplasty(PKP)
Authors: Xu Jia-Jing , Lin Yan , Wang Yi-Han , Zhu Ding-Chao , Wu Yao-Sen 
Published: July 2020

Abstract
Background: Psychological disorder is widespread in patients with spinal conditions. There is limited investigation into the association between preoperative psychological symptoms(PPS) and postoperative outcomes following percutaneous kyphoplasty(PKP) in patients suffered osteoporotic vertebral compression fracture(OVCF) . Methods: This is a retrospective study performed on 224 patients undergoing PKP due to OVCF. Patients are divided into two cohorts base on preoperative psychological states described by Hamilton Depression Scale(HAMD) and Hamilton Anxiety Scale(HAMA). Pre- and postoperative physical function were evaluated by Patient-Reported Outcome Measurement Information System Physical Function(PROMIS PF), and Visual Analogue Scales(VAS) was used to to evaluate patients' back pain. Multiple linear regression model was used to investigate the association between preoperative psychological symptoms and postoperative outcomes.Results: 133(59.4%) patients have PPS, manifested as HAMD score ≥7 and/or HAMA score＞7. Patients with PPS have significantly worse PROMIS PF scores preoperatively (26.6±7.6 vs. 34.2±6.9, p=0.000), as well as within 6 monthes postoperatively, 1-week (31.1±6.5 vs. 37.4±7.2, p=0.000), 1-month (35.3±6.2 vs. 41.1±7.4, p=0.023), 3-months (38.5±8.3 vs. 45.8±8.5, p=0.005), and 6-months (42.8±8.0 vs. 51.2±9.1, p=0.000). Patients with PPS have higher VAS scores preoperatively(8.3±1.6 vs. 6.9±1.9, p=0.001), as well as within 1 month following operation, 1-week (6.2±1.7 vs. 4.1±1.2, p=0.000), 1-month (4.0±1.1 vs. 2.5±1.0, p=0.015).Conclusion: Psychological symptoms are widespread in OVCF patients undergoing PKP. Patients with poor preoperative psychological states not only demonstrated worse preoperative physical function and back pain, but also continued to have significantly worse postoperative outcomes.
An Exploratory Study of Consumers’ Social Behavior Towards Role of Malaysian Community Pharmacists in Providing Healthcare
Authors: Alabid Alamin Hassan Mohamed Alamin , Ibrahim Mohamed Izham Mohamed , Hassali Mohamed Azmi , Palaian Subish 
Published: July 2020

Abstract
Background: Consumers awareness, perceptions, expectations and experiences are integral components of their satisfaction towards the quality of care provided by community pharmacists. The study aims to assess consumers’ awareness, perceptions, expectations and experiences about the role of community pharmacists in providing healthcare.Methods: The study was carried out in the state of Penang, in the north of Malaysia. A survey was carried out by conveniently selecting the consumers patronizing the community pharmacies. A pretested and validated survey tool with different domains (Cronbach’s alpha ranged from 0.577 to 0.774) was used. Descriptive and inferential statistics (Chi-Square, Mann-Whitney and Kruskal-Wallis tests) were used for analyzing the data using SPSS program, Version 26.Results: Of the total 424 questionnaires distributed, 195 (46%) were returned and usable. The median age of the consumers was 30 (IQR = 28-38). The majority of them were female, Chinese, living in urban area and having a bachelor degree. The mean awareness score was 8.39 (± 2.00), mean perception score was 2.95 (± 0.83), mean expectation score was 3.70 (± 0.13), and mean experience score was 3.17 (± 0.97). Trusting a pharmacist’s advice about medicines was significantly associated with the consumer’s age (p= 0.030). age of the consumers was a significant determinant in their perception of CP suggestions about the use of certain prescription medicines to patients and physicians (p = .001 and p = 0.014, successively). The strongly anticipated that CPs should monitor responses to drug therapy and be more concerned with the patient rather than being profit-oriented (medians = 5 and 4, respectively).Conclusions: The consumers in this study were aware of the role of community pharmacists in community pharmacies and healthcare settings. They were also showed high perception, expectation and positive experience with the community pharmacists.
TERT Mutation was Associated with the Risk of Recurrence in Lung Adenocarcinoma with A Micropapillary Pattern
Authors: Wang Fenfang , Xu Lu , Hao Qing , Li Chenghui , Wu Qihuan , Lu Hongyang 
Published: July 2020

Abstract
Background: Lung adenocarcinoma with a micropapillary pattern (MPPAC) is the histological subtype of lung cancer. It has attracted increasing attention, especially regarding its association with poor prognosis, including the predisposition towards recurrence and metastasis. Although MPPAC has been described in early-stage cases, only a few studies have reported the correlation between disease-specific prognosis and gene mutation of MPPAC. This study aimed to clarify the common genetic mutations and the prognostic characteristics in MPPAC patients.Methods: A total of 17 patients whose surgical pathology was defined as MPPAC were followed up, the molecular characteristics were elucidated by next-generation sequencing, and the prognostic characteristics were analyzed. Results: Epidermal growth factor receptor (EGFR) mutations were identified in 11/17 (65%) of patients. TP53 alterations were identified in 10/17 (59%). Other common mutations include ATM (18%), KRAS (18%), SDHA (18%), and TERT (18%). MPPAC patients harboring EGFR and TERT mutations were at a high risk of tumor recurrence, while TP53 might be associated with a low risk of recurrence. Conclusions: TERT mutation was more frequently harbored in MPPAC patients than in the other histological type of lung cancer, and such patients were at a high risk of recurrence. So TERT mutation might be associated with adverse prognosis in MPPAC patients.
Nomogram-Derived Prediction of Pathologic Complete Response (pCR) in Breast Cancer Patients Treated with Neoadjuvant Chemotherapy (NCT)
Authors: pu shengyu , wang ke , liu yang , liao xiaoqin , chen heyan , he jianjun , zhang jian 
Published: July 2020

Abstract
Background Previous research results on the predictive factors of neoadjuvant chemotherapy (NCT) efficacy in breast cancer are inconsistent, suggesting that the ability of a single factor to predict efficacy is insufficient. Combining multiple potential efficacy-related factors to build a model may improve the accuracy of prediction. This study intends to explore the clinical and biological factors in breast cancer patients receiving NCT and to establish a nomogram that can predict the pathologic complete response (pCR) rate of NCT.Methods We selected 165 breast cancer patients receiving NCT from July 2017 to May 2019. Using pretreatment biopsy materials, immunohistochemical studies to assess estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER-2), and Ki-67 expression. The correlation between biological markers and pCR was analyzed. These predictors were used to develop a binary logistic regression model with cross-validation and to show the established predictive model with a nomogram.Results The nomogram for pCR based on lymphovascular invasion, anemia (HB≤120 g/L), ER, Ki67 expression levels and NCT regimen had good discrimination performance (area under the curve [AUC], 0.758; 95% confidence interval [CI], 0.675-0.841) and calibration coordination. According to the Hosmer-Lemeshow test, the calibration chart showed satisfactory agreement between the predicted and observed probabilities. The final prediction accuracy of cross-validation was 76%.Conclusions We developed a nomogram based on multiple clinical and biological covariations that can provide an early prediction of NCT response and can help to quickly assess the individual benefits of NCT.
Developing a Complex Intervention to Support Pregnant Women with Mild to Moderate Anxiety: Application of the Medical Research Council Framework
Authors: Evans Kerry , Spiby Helen , Morrell Jane C 
Published: July 2020

Abstract
Aim: To design and develop an intervention to support women with symptoms of mild to moderate anxiety in pregnancy.Methods: The development followed the MRC framework for complex interventions, utilising psychological theory, review level evidence and professional and public involvement. Two systematic reviews were completed which helped identify potentially beneficial intervention components. The theory underpinning the components was explored to consider the potential benefit for women with mild to moderate anxiety symptoms in pregnancy. Methods of delivering the intervention within maternity services were explored. The intervention comprised: group discussions, one to one support and assisted self-help resources. Midwives were identified as ideally placed to facilitate the intervention supported by midwifery support workers. A bespoke training package was provided by subject experts to prepare the facilitators. Discussion: The absence of established interventions and a paucity of evidence based approaches for pregnant women with symptoms of mild to moderate anxiety indicated the need for a rigorous and systematic approach to the intervention design. This approach led to the development of an intervention feasible for implementation in maternity care systems tailored to the needs of pregnant women. The involvement of a multi-professional advisory team and active engagement of service users helped to consider the acceptability of the intervention for women and the feasibility of delivering the intervention in the context of maternity care.Conclusion: The MRC Framework provided useful overarching guidance to develop a midwife facilitated intervention for women with symptoms of anxiety in pregnancy. The framework assisted the development of a robust rationale for each intervention component and considered the processes of evaluation and implementation into maternity care systems.
Lack of Tocilizumab effect on mortality in COVID19 patients
Authors: Holt Gregory E , Batra Mayank , Murthi Mukunthan , Kambali Shweta , Santos Kayo , Bastidas Maria Virginia Perez , Asif Huda , Haddadi Sara , Arias Sixto , Mirsaeidi Mehdi 
Published: July 2020

Abstract
Off-label tocilizumab use in COVID-19 patients reflects concern for cytokine release syndrome. Comparison of matched COVID-19 pneumonia patients found elevated IL-6 levels correlated with mortality that did not change with tocilizumab administration. Correlating mortality with increased IL-6 doesn’t imply causality however lack of improvement by tocilizumab requires clinical trial alterations.
Recurrence of COVID-19 with SARS-CoV-2 RNA reversion to positive and late antibody response: a case report from Brazil
Authors: Alonso Fábio de O. Martinez , Sabino Bruno Duarte , Guimarães Maria Angelica Arpon Marandino , Varella Rafael Brandão 
Published: July 2020

Abstract
In general, replication of the SARS-CoV-2 in the host reaches its peak in the first week of infection, decreasing rapidly afterwards, while some level of immunity is build up. Yet, the infection seems to follow a distinctive course in some individuals, reactivating after the apparent resolution of symptoms. We report here the first case of a more vigorous COVID-19 recurrence with SARS-CoV-2 RNA reversion to positive and late antibody response, and also the first to address COVID-19 recurrence in Brazil.
Association of hyperlipidemia and statin use with severity of COVID-19
Authors: Tan Wilnard YT , Young Barnaby E , Lye David Chien , Chew Daniel EK , Dalan Rinkoo 
Published: July 2020

Abstract
Background and Aims: We aim to study the association of hyperlipidemia and statin use with COVID-19 severity.Methods: We analysed a retrospective cohort of 717 patients admitted to a tertiary centre in Singapore for COVID-19 infection. Clinical outcomes of interest were oxygen saturation ≤94% requiring supplemental oxygen, intensive-care unit (ICU) admission , invasive mechanical-ventilation and death. Logistic regression models were used to study the association between hyperlipidemia and clinical outcomes adjusted for age, gender and ethnicity. Statin treatment effect was determined , in a nested case-control design, through logistic treatment models with 1:3 propensity matching for age, gender and ethnicity. All statistical tests were two-sided, and statistical significance was taken as p < 0.05.Results: One hundred fifty-six (21.8%) patients had hyperlipidemia and 97% were on statins. There were no significant associations between hyperlipidemia and clinical outcomes. Logistic treatment models showed a lower chance of ICU admission for statin users when compared to non-statin users (ATET: b-0.12(-0.23,-0.01); p=0.028). There were no other significant differences in other outcomes.Conclusion: Treated hyperlipidemia was not an independent risk factor for severe COVID-19. Statin use independently associated with lower ICU admission. This supports current practice to continue prescription of statins in COVID-19 patients.