bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional divergence and adaptive selection of KNOX gene family in plants
Authors: Meng Lingyan , Liu Xiaomei , He Congfen , Xu Biyao , Li Yaxuan , Hu Yingkao 
Published: June 2020

AbstractKNOTTED-like homeodomain (KNOX) genes are transcriptional regulators that play an important role in morphogenesis. In the present study, a comparative analysis was performed to investigate the molecular evolution of the characteristics of theKNOXgene family in 10 different plant species. We identified 129KNOXgene family members, which were categorized into two subfamilies based on multiple sequence alignment and phylogenetic tree reconstruction. Several segmental duplication pairs were found, indicating that different species share a common expansion model. Functional divergence analysis identified the 15 and 52 amino acid sites with significant changes in evolutionary rates and amino acid physicochemical properties as functional divergence sites. Additional selection analysis showed that 14 amino acid sites underwent positive selection during evolution, and two groups of co-evolutionary amino acid sites were identified by Coevolution Analysis using Protein Sequences software. These sites could play critical roles in the molecular evolution of theKNOXgene family in these species. In addition, the expression profiles ofKNOXduplicated genes demonstrated functional divergence. Taken together, these results provide novel insights into the structural and functional evolution of theKNOXgene family.
MiR-223-3p regulates cell viability, migration, invasion, and apoptosis of non-small cell lung cancer cells by targeting RHOB
Authors: Li Shufang , Feng Yuping , Huang Yuxia , Liu Yu , Wang Yanxi , Liang Yan , Zeng Hui , Qu Hong , Wei Ling 
Published: June 2020

Abstract
Non-small cell lung cancer (NSCLC) is the most common type of lung cancer with a high fatality rate in men and women worldwide. Recently, microRNAs (miRNAs) have been reported to be diagnostic biomarkers and therapeutic targets in NSCLC. MiR-223-3p was proved to act as a promoter in NSCLC progression. However, the regulatory mechanism of miR-223-3p in NSCLC remains little known. This study aimed to explore the regulatory mechanism between miR-223-3p and its target gene Ras homolog family member B (RHOB) in NSCLC. The mRNA level of miR-223-3p and RHOB was measured by quantitative reverse transcription PCR. Furthermore, cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Flow cytometry was conducted to analyze cell apoptosis. Transwell assays and wound healing assay were employed to examine migration and invasion. The target relationship between miR-223-3p and RHOB was predicted by starBase online database and verified by dual-luciferase assay. The protein level of RHOB was tested by western blot. Our data suggested that miR-223-3p was upregulated in NSCLC tissues and cell lines and high level of miR-223-3p contributed to a poor survival in NSCLC patients. Knockdown of miR-223-3p exerted inhibitory effects on NSCLC cell viability, migration, and invasion and promotion effect on cell apoptosis. Furthermore, RHOB was directly targeted by miR-223-3p and constrained NSCLC progression. Moreover, knockdown of RHOB rescued the effect of anti-miR-223-3p on NSCLC progression. In vivo experiments indicated that miR-223-3p deletion suppressed tumor growth. MiR-223-3p could regulate the NSCLC cellular processes through targeting RHOB.
Eccentricity based topological indices of siloxane and POPAM dendrimers
Authors: Iqbal Muhammad Azhar , Imran Muhammad , Zaighum Muhammad Asad 
Published: June 2020

Abstract
A massive of early drug tests indicates that there is some strong inner connections among the bio-medical and pharmacology properties of nanostar dendrimers and their molecular structures. Topological descriptors are presented as fundamentally transforming a molecular graph into a number. There exist various categories of such descriptors particularly those descriptors that based on edge and vertex distances. Topological descriptors are exercised for designing biological, physico-chemical, toxicological, pharmacologic and other characteristics of chemical compounds. In this paper, we study infinite classes of siloxane and POPAM dendrimers and derive their Zagreb eccentricity indices, eccentric-connectivity and total-eccentricity indices.
Towards an Anxiety-Buffer Disruption Approach to Depression: Attachment Anxiety and Worldview Threat Heighten Death-Thought Accessibility and Depression-Related Feelings
Authors: Mikulincer Mario , Lifshin Uri , Shaver Phillip R. 
Published: June 2020

Introduction: In two studies, we tested an anxiety-buffer disruption approach to depression, examining the effects of attachment insecurities, worldview threat, and death concerns on depression-related feelings.Method: In both studies, Israeli undergraduates reported on their attachment insecurities (anxiety, avoidance), were exposed to a worldview threat or a no-threat condition, and then rated their current level of depression-related feelings.Results: In Study 1 (N = 124), we also measured death-thought accessibility and found that a worldview threat (versus no-threat) heightened death-thought accessibility and depression feelings only among participants scoring relatively high on attachment anxiety, and that death-thought accessibility mediated the effects of worldview threat and attachment anxiety on feelings of depression. In Study 2 (N = 240), we randomly assigned participants to a mortality salience or a control condition and found that heightened death concerns caused more depression only when a worldview threat was present and participants' attachment anxiety was high.Discussion: The roles that disruption of anxiety buffering systems and death-related concerns play in depression were discussed.
Genome Report: Whole Genome Sequence and Annotation of the Parasitoid Jewel Wasp Nasonia giraulti Laboratory Strain RV2X[u]
Authors: Wang Xiaozhu , Kelkar Yogeshwar D , Xiong Xiao , Martinson Ellen O , Lynch Jeremy , Zhang Chao , Werren John H , Wang Xu 
Published: June 2020

Abstract
Jewel wasps in the genus of Nasonia are parasitoids with haplodiploidy sex determination, rapid development and are easy to culture in the laboratory. They are excellent models for insect genetics, genomics, epigenetics, development, and evolution. Nasonia vitripennis (Nv) and N. giraulti (Ng) are closely-related species that can be intercrossed, particularly after removal of the intracellular bacterium Wolbachia, which serve as a powerful tool to map and positionally clone morphological, behavioral, expression and methylation phenotypes. The Nv reference genome was assembled using Sanger, PacBio and Nanopore approaches and annotated with extensive RNA-seq data. In contrast, Ng genome is only available through low coverage resequencing. Therefore, de novo Ng assembly is in urgent need to advance this system. In this study, we report a high-quality Ng assembly using 10X Genomics linked-reads with 670X sequencing depth. The current assembly has a genome size of 259,040,977 bp in 3,160 scaffolds with 38.05% G-C and a 98.6% BUSCO completeness score. 97% of the RNA reads are perfectly aligned to the genome, indicating high quality in contiguity and completeness. A total of 14,777 genes are annotated in the Ng genome, and 72% of the annotated genes have a one-to-one ortholog in the Nv genome. We reported 5 million Ng-Nv SNPs which will facility mapping and population genomic studies in Nasonia. In addition, 42 Ng-specific genes were identified by comparing with Nv genome and annotation. This is the first de novo assembly for this important species in the Nasonia model system, providing a useful new genomic toolkit.
Modos discursivos de acceder a una comunidad disciplinar
Authors: Musci Mónica Beatriz , Muñóz Nora Isabel 
Published: June 2020

La argumentación disciplinar es una práctica discursiva que involucra procesos de indagación, construcción y comunicación de conocimientos. Lejos de ser una práctica homogénea, las disciplinas científicas “varían tanto en los tipos de problemas que indagan como en las formas de argumentación que adoptan” (Hyland, 2012: 31). Dado que los discursos académicos son medios privilegiados para integrar a los aprendices a sus comunidades disciplinares y profesionales (Parodi, 2010), el aprendizaje de esta práctica se vuelve especialmente relevante para la inclusión efectiva de nuevos miembros. Presentamos en este trabajo un análisis de trabajos escritos de alumnos avanzados de Psicopedagogía e Ingeniería Química de la UNPA centrando nuestra atención en los esquemas argumentativos predominantes que elaboran, los tipos de evidencia que utilizan y los modos de dialogar con la tradición discursiva a la que pertenecen. El análisis muestra algunas notas distintivas de los diferentes modos en que los estudiantes construyen una cierta identidad disciplinar.
Assessing atomically thin delta-doping of silicon using mid-infrared ellipsometry
Authors: Katzenmeyer Aaron M. , Luk Ting S. , Bussmann Ezra , Young Steve , Anderson Evan M. , Marshall Michael T. , Ohlhausen James A. , Kotula Paul , Lu Ping , Campbell DeAnna M. , Lu Tzu-Ming , Liu Peter Q. , Ward Daniel R. , Misra Shashank 
Published: June 2020

Abstract
Fibroblast Growth Factor 23 and Risk of Hospitalization with Infection in Chronic Kidney Disease: The Chronic Renal Insufficiency Cohort (CRIC) Study
Authors: Ishigami Junichi , Taliercio Jonathan T. , Feldman Harold I. , Srivastava Anand , Townsend Raymond R. , Cohen Debbie L. , Horwitz Edward J. , Rao Panduranga , Charleston Jeanne , Fink Jeffrey C. , Ricardo Ana C. , Sondheimer James , Chen Teresa K. , Wolf Myles , Isakova Tamara , Appel Lawrence J. , Matsushita Kunihiro ,   for the CRIC study Investigators
Published: June 2020

Significance Statement
Association of fibroblast growth factor 23 (FGF23) with risk of infection has not been evaluated in a CKD population. Among 3655 participants of the Chronic Renal Insufficiency Cohort (CRIC) study who had nondialysis-dependent CKD, higher baseline plasma levels of C-terminal FGF23 were significantly and monotonically associated with time to first hospitalization with major infection, independent of biomarkers of inflammation or bone mineral metabolism. These findings suggest a possible role of FGF23 in the increased susceptibility to infection among individuals with CKD. The most frequent infections were of the urinary tract, followed by cellulitis/osteomyelitis, pneumonia, and then bacteremia/septicemia.

Background
Risk of infectious disease is increased among individuals with CKD. Fibroblast growth factor 23 (FGF23) is often elevated in CKD, and may impair immune function directly or indirectly through proinflammatory and vitamin D–suppressing pathways. Whether FGF23 is associated with risk of infection has not been evaluated in a CKD population.

Methods
In 3655 participants of the Chronic Renal Insufficiency Cohort study, we evaluated the association of baseline plasma levels of C-terminal FGF23 with time to first hospitalization with major infection, defined by hospital discharge with a diagnosis code for urinary tract infection, pneumonia, cellulitis/osteomyelitis, or bacteremia/septicemia. Multivariable Cox models were used to estimate hazard ratios (HRs) and adjust for confounding.

Results
During a median follow-up of 6.5 years, 1051 individuals (29%) were hospitalized with major infection. Multivariable Cox analysis indicated a graded increase in the risk of infection with higher levels of FGF23 (HR, 1.51; 95% CI, 1.23 to 1.85 with the highest quartile [≥235.9 RU/ml] versus lowest quartile [<95.3 RU/ml]; HR, 1.26; 95% CI, 1.18 to 1.35 per SD increment in log FGF23). The association was consistent across infection subtypes and demographic and clinical subgroups, and remained significant after additional adjustment for biomarkers of inflammation (IL-6, TNF-α, high-sensitivity C-reactive protein, fibrinogen, and albumin), and bone mineral metabolism (25-hydroxyvitamin D, phosphorus, calcium, and parathyroid hormone). The association was consistent across infection subtypes of urinary tract infection (482 cases), cellulitis/osteomyelitis (422 cases), pneumonia (399 cases), and bacteremia/septicemia (280 cases).

Conclusions
Among individuals with CKD, higher FGF23 levels were independently and monotonically associated with an increased risk of hospitalization with infection.
AVALIAÇÃO FÍSICO-QUÍMICA E LEGISLAÇÃO BRASILEIRA DE POLPAS, SUCOS TROPICAIS E NÉCTARES DE GOIABA COMERCIAIS
Authors: Nogueira Andressa Milene Parente , Figueira Ricardo , Imaizumi Vitor Massami , Venturini Filho Waldemar Gastoni 
Published: June 2020

AVALIAÇÃO FÍSICO-QUÍMICA E LEGISLAÇÃO BRASILEIRA DE POLPAS, SUCOS TROPICAIS E NÉCTARES DE GOIABA COMERCIAIS

ANDRESSA MILENE PARENTE NOGUEIRA1, RICARDO FIGUEIRA2, VITOR MASSAMI IMAIZUMI3, WALDEMAR GASTONI VENTURINI FILHO4

1Departamento de Produção Vegetal, Faculdade de Ciências Agronômicas. Avenida Universitária, n°3780, Altos do Paraíso, CEP. 18610-034, Botucatu, São Paulo, Brasil. andressa_nogueira@yahoo.com.br
2Departamento de Produção Vegetal, Faculdade de Ciências Agronômicas. Avenida Universitária, n°3780, Altos do Paraíso, CEP. 18610-034, Botucatu, São Paulo, Brasil. ricardo.figueira@unesp.br
3Departamento de Produção Vegetal, Faculdade de Ciências Agronômicas. Avenida Universitária, n°3780, Altos do Paraíso, CEP. 18610-034, Botucatu, São Paulo, Brasil. vtr_massami@hotmail.com
4Departamento de Produção Vegetal, Faculdade de Ciências Agronômicas. Avenida Universitária, n°3780, Altos do Paraíso, CEP. 18610-034, Botucatu, São Paulo, Brasil. waldemar.venturini@unesp.br

RESUMO: Este trabalho teve como objetivos avaliar, por meio de análises físico-químicas, polpas, sucos tropicais e néctares de goiaba e averiguar se os mesmos atendem aos Padrões de Identidade e Qualidade (PIQ) exigidos pelo Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Foram analisadas três polpas, sete sucos tropicais (quatro não adoçados e três adoçados) e oito néctares (cinco convencionais e três light) quanto ao teor de sólidos solúveis (ºBrix), pH, acidez total, açúcares totais e ácido ascórbico (vitamina C). Seguindo os parâmetros determinados por lei, as polpas também foram analisadas para concentração de sólidos totais e ratio. Os dezoito produtos analisados estavam de acordo com a legislação em vigor.

Palavras-chave: Psidium guajava, parâmetros físico-químicos, legislação brasileira.

PHYSICAL-CHEMICAL EVALUATION AND BRAZILIAN LEGISLATION OF COMMERCIAL GUAVA PULP, TROPICAL JUICES AND NECTARS

SUMMARY: This work aimed to evaluate physico-chemical guava pulps, tropical juices and nectars to ascertain whether they met the Identity and Quality Standards required by Brazilian legislation. Three pulps, seven tropical juices (four unsweetened and three sweetened) and eight nectars (five conventional and three light) were analyzed for soluble solids (ºBrix), pH, total acidity, total sugars and ascorbic acid (vitamin C). Following the parameters set by law, the pulps were also analyzed for total solids concentration and ratio. The eighteen products analyzed were in according to the legislation.

Keyword: Psidiumguajava, physical-chemicalparameters, brazilianlegislation.
LITERATURA, ENSINO E AS TICs
Authors: Ferreira Beatriz Pazini 
Published: June 2020

A pós-modernidade oportuniza sensações de liberdade na criação e no rompimento com o tradicional. Atualmente, podemos caracterizá-la como “era da liquefação do projeto moderno”, pois a incorporação de novas tecnologias computacionais de comunicação possibilita o desenvolvimento dos ambientes virtuais como novos meios de apoio ao aprendizado, às trocas de informações, à comunicação, à interação e à disponibilização de material de estudo que agregam e disseminam interação. Então, entendemos que as TICs podem ser “espaços” que oportunizam ao discente se apropriar efetivamente das experiências estéticas virtuais fruindo-as, retendo-as. Dessa forma, o objetivo do texto é abordar o ensino de literatura em uma perspectiva pós-moderna apresentando de que forma é possível oferecer condições para que o aluno tenha competência linguística literária e exerça, por meio da associação entre literatura e as novas tecnologias, consciência em relação às práticas sociais. Para isso, utilizamos abordagens explicativas em relação à definição e à aplicação do livroclip, visto que estes contêm transposição e representação dramatizada de obras literárias sendo interativa e digital. Aproveitamos as lições Bakhtin, Aguiar e Bordini (1983), Rocco (1992), Kleiman (2004) e Zappone (2008).
The M2 Tidal Tilt Results from USArray Seismic Data from the Western United States
Authors: Martynov Vladislav G. , Astiz Luciana , Kilb Debi , Vernon Frank L. 
Published: June 2020

ABSTRACTWe explore the detectability of M2 tidal tilt in the western part of the United States, using seismic velocity data from 40 stations in the EarthScope Transportable Array (TA) network. We augment these data with data from two additional stations both collocated at the Piñon Flats Observatory (PFO) in southern California (networks TA and Incorporated Research Institutions for Seismology [IRIS] International Deployment of Accelerometers [IDA]). We find a good agreement between the acceleration-tilt derived from the TA seismic data with the theoretical model (body Earth and ocean loading for M2). These results are also consistent with prior studies using borehole tiltmeters operated at PFO (Wyatt and Berger, 1980; Wyatt et al., 1982). We find statistically significant M2 tilt anomaly responses that correlate with large lateral variations in rock properties in Yellowstone National Park, which stem from volcanic sources in the region. We also examined deviations in the M2 tidal tilt mode in regions with other geological features including the Cascades volcanic range and a large plutonic body located in Idaho and eastern Oregon. Of these, only the Cascadia data show evidence of lateral variances of elastic properties, similar to that of the Yellowstone Caldera (YC). We conclude that tilt measurements from seismic noise data can successfully identify relatively large structural changes in elastic properties of the crustal Earth (e.g., the YC) and significant change in the elastic properties (e.g., Cascadia subduction zone). But, when the features are smaller and/or have a more muted variation in the elastic properties (e.g., the plutonic body in Idaho and eastern Oregon), the induced changes in the tilt values are too small to be detected using TA data.
Emerging pandemic COVID-19: myths and facts
Authors: Vadala Rohit , Princess Isabella 
Published: June 2020

<p>The first theory which has established itself across the world is that COVID-19 is a “new virus”. It is rather wise to call it a “new strain” of a pre-existing coronavirus since history clearly denotes cases of coronavirus surfacing the world in past years beginning as early as mid-1960s.Including this novel strain of the virus, seven strains of coronaviruses have been commonly associated with human infections. Coronaviruses are primarily respiratory viruses causing infections ranging from mild to severe involvement of the respiratory tract. The common cold strains of coronavirus are 229E alpha coronavirus, NL63 alpha coronavirus, OC43 beta coronavirus and HKU1 beta coronavirus.The acute respiratory distress causing strains are severe acute respiratory syndrome (SARS) beta CoV causing SARS, MERS beta CoV causing Middle East respiratory syndrome (MERS) and the very novel COVID-19. Researchers and molecular biologists have confirmed phylogenetic relationship of COVID-19 with a 2015 Chinese bat strain of SARS CoV.<sup> </sup>Mutations to the surface protein as well as nucleocapsid proteins were demonstrated. These two mutations predicts the characteristics such as higher ability to infect as well as enhanced pathogenicity of COVID-19 as compared to older SARS strain. For this reason and with similarities in clinical presentation the novel strain has been named as SARS-CoV-2.</p>
Emocje jako element struktury przekazu samorządu lokalnego w serwisie Facebook.com. Studium przypadku oficjalnego profilu Miasta Stołecznego Warszawy
Authors: Kowalik Krzysztof 
Published: June 2020

Serwisy społecznościowe są coraz istotniejszym narzędziem komunikacji instytucji publicznych. Samorządy lokalne (władze gmin — miast) tworzą oficjalne profile społecznościowe, aby docierać nie tylko do swoich mieszkańców, ale także do turystów, inwestorów. Publikują treści o zróżnicowanym charakterze i zawartości. Z badań autora wynika, że przekaz ten można kategoryzować. Każdy rodzaj treści niesie ze sobą nie tylko porcję informacji, ale niektóre kategorie postów zawierają elementy, które mają za zadanie wzbudzać emocje i zarazem zaangażowanie użytkowników samorządowego profilu. Taki przekaz może zwiększać zasięg publikacji. Autor prezentuje wyniki swoich badań, wskazujących na preferowanie przez samorząd treści mogących inicjować wzmożoną, emocjonalną interakcję użytkowników serwisu Facebook.com. Studium przypadku (oficjalnego profilu Miasta Stołecznego Warszawy) stanowi próbę wskazania tych elementów struktury przekazu (postów), które mogą wzbudzać poruszenie wyrażane online w postaci „lajków”, komentarzy lub „szerowania”.
O formach zawierania małżeństw i statusie kobiet na Rusi w czasach pogańskich
Authors: Kosowska Katarzyna 
Published: June 2020

Celem niniejszego artykułu jest próba znalezienia odpowiedzi na pytanie, jakie formy zawierania małżeństwa oraz obrzędy ślubne znała Ruś okresu pogańskiego. Artykuł stanowi również próbę przedstawienia społecznego oraz majątkowego statusu kobiet staroruskich w badanych okresie. W charakterze źródeł badawczych wykorzystane zostały zabytki piśmiennictwa staroruskiego — latopisy: Powieść minionych lat oraz Latopis Pieriejasławia-Suzdalskiego.
Analiza staroruskich latopisów pozwoliła na wyodrębnienie dwóch, zasadniczo różniących się sposobów zawierania małżeństwa w okresie przedchrześcijańskim: „porwania” („умычка”) oraz „prowadzenia” („ведение”) żony. Ponadto istnieje możliwość rekonstrukcji trzeciej, pośredniej formy zawierania małżeństwa — kupno-sprzedaż narzeczonej („купля-продажа”). Siła więzów małżeńskich oraz status kobiet w rodzinie pogańskiej były pochodną formy zawartego małżeństwa.
Bellows in Malaga: Thomistic Insights via Pablo Picasso
Authors: Fitzpatrick Daniel 
Published: June 2020

Miechy w Maladze: tomistyczny ogląd na podstawie Picassa
Artykuł rozpoczyna się od wyjaśnienia nauczania św. Tomasza na temat problemu ‘jedno i wiele’ poprzez udzielenie odpowiedzi na trzy pytania: 1) Co to jest rodzaj? 2) W jaki sposób, na bazie przeciwieństw, zorganizowane są rodzaje i jaką rolę odgrywa w takiej organizacji ilość wirtualna? 3) W jaki sposób poznający i to, co poznawane, stanowią przeciwstawne bieguny danego rodzaju? Bazując na odpowiedziach na te pytania, przechodzimy następnie do analizy sztuki, ze szczególnym uwzględnieniem Picassa, w celu wyjaśnienia trzech uzupełniających się punktów: 1) W jaki sposób artysta i jego dzieło stanowią rodzaj i w jaki sposób dzieło i odbiorca stanowią rodzaj; 2) W jaki sposób dzieło sztuki potwierdza rodzajową relację zmysłowego przedmiotu i zmysłowego bytu; 3) W jaki sposób artysta podporządkowuje to, co praktyczne, spekulacyjne, swojej pracy i co to oznacza dla roli artysty w epoce coraz bardziej nastawionej na praktyczną stronę życia.