bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct microalgae species for food—part 1: a methodological (top-down) approach for the life cycle assessment of microalgae cultivation in tubular photobioreactors
Authors: Schade S. , Meier T. 
Published: July 2020

AbstractSpecific microalgae species are an adequate source of EPA and DHA and are able to provide a complete protein, which makes them highly interesting for human nutrition. However, microalgae cultivation has also been described to be energy intensive and environmentally unfavorable in pilot-scale reactors. Moreover, production in cold temperature zones has not been sufficiently investigated. In particular, the effects of tube materials and cultivation season length have rarely been previously investigated in the context of a comparative LCA of microalgae cultivation. A computational “top-down” model was conducted to calculate input flows for Nannochloropsis sp. and Phaeodactylum tricornutum cultivation in a hypothetical tubular photobioreactor. Cultivation processes were calculated according to detailed satellite climatic data for the chosen location in Central Germany. This model was applied to a set of different scenarios, including variations in photobioreactor material, tube diameter, microalgae species, and cultivation season length. Based on these data, a life cycle assessment (LCA) was performed following ISO standard 14040/44. The impact assessment comprised the global warming potential, acidification, eutrophication, cumulative energy demand, and water scarcity. The results showed that a long cultivation season in spring and fall was always preferable in terms of environmental impacts, although productivity decreased significantly due to the climatic preconditions. Acrylic glass as a tube material had higher environmental impacts than all other scenarios. The cultivation of an alternative microalgae species showed only marginal differences in the environmental impacts compared with the baseline scenario. Critical processes in all scenarios included the usage of hydrogen peroxide for the cleaning of the tubes, nitrogen fertilizer, and electricity for mixing, centrifugation, and drying. Microalgae cultivation in a tubular photobioreactor in a “cold-weather” climate for food is sustainable and could possibly be a complement to nutrients from other food groups. The added value of this study lies in the detailed description of a complex and flexible microalgae cultivation model. The new model introduced in this study can be applied to numerous other scenarios to evaluate photoautotrophic microalgae cultivation in tubular photobioreactors. Thus, it is possible to vary the facility location, seasons, scale, tube dimensions and material, microalgae species, nutrient inputs, and flow velocity. Moreover, single processes can easily be complemented or exchanged to further adjust the model individually, if, for instance, another downstream pathway is required.
Modeling multi-tier heterogeneous small cell networks: rate and coverage performance
Authors: Fadoul Moubachir Madani 
Published: July 2020

AbstractThe rapid growth of small cells is driving cellular network toward randomness and heterogeneity. The multi-tier heterogeneous network (HetNet) addresses the massive connectivity demands of the emerging cellular networks. Cellular networks are usually modeled by placing each tier (e.g macro, pico and relay nodes) deterministically on a grid which ignores the spatial randomness of the nodes. Several works were idealized for not capturing the interference which is a major performance bottleneck. Overcoming such limitation by realistic models is much appreciated. Multi-tier relay cellular network is studied in this paper, In particular, we consider $${\mathscr {K}}$$
K
-tier transmission modeled by factorial moment and stochastic geometry and compare it with a single-tier, traditional grid model and multi-antenna ultra-dense network (UDN) model to obtain tractable rate coverage and coverage probability. The locations of the relays, base stations, and users nodes are modeled as a Poisson Point Process. The results showed that the proposed model outperforms the traditional multi-antenna UDN model and its accuracy is confirmed to be similar to the traditional grid model. The obtained results from the proposed and comparable models demonstrate the effectiveness and analytical tractability to study the HetNet performance.
Genetic analysis of callus formation in a diversity panel of 96 rose genotypes
Authors: Nguyen Thi Hong Nhung , Winkelmann Traud , Debener Thomas 
Published: July 2020

AbstractIn a diversity panel of 96 rose genotypes, variation in the capacity to form calluses on leaf explants in vitro was investigated, and a genome-wide association study (GWAS) was performed to identify genetic factors associated with callus formation. Calluses were induced from wounded in vitro leaflets on two media differing in their plant growth regulator composition. Significant differences between genotypes were observed in callus size on the first callus-inducing medium (CIM1, containing 10.7 µM naphthylene acetic acid) using a 0–4 scale, as well as on a second callus-inducing medium (CIM2, containing 4.5 µM dichlorophenoxyacetic acid and 2 µM 6-(γ,γ-dimethylallylaminopurine)) with callus size scales of 0.82–4. GWAS utilizing the WagRhSNP 68K SNP array for callus size induced on either CIM1 or CIM2 enabled the identification of 26 and 13 significantly associated SNPs, respectively. Among these SNPs, we found the SNPs Rh12GR_12098_1092Q (uncharacterized gene) and RhMCRND_2903_1233Q in a gene encoding a pentatricopeptide repeat-containing protein were associated with callus size on CIM1, with large effects being observed between alleles. Two SNPs, RhK5_5473_763P (S-formylglutathione hydrolase) and Rh12GR_37799_568Q (polyglutamine binding protein, WW domain binding protein), were associated with callus size on CIM2 with large effect sizes. The markers associated with callus size on CIM1 form a large cluster on chromosome 3 and minor clusters on other chromosomes and provide the first preliminary indications of candidate genes responsible for the observed phenotypic variation.
Comparative study on analytical and computational aerodynamic models for flapping wings MAVs
Authors: Valdez M.F. , Balachandran B. , Preidikman S. 
Published: July 2020

ABSTRACTA range of quasi-steady and unsteady aerodynamic models are used to predict the aerodynamic forces experienced by a flapping wing and a detailed comparison amongst these predictions in provided. The complexity of the models ranges from the analytical potential flow model to the computational Unsteady Vortex Lattice Method (UVLM), which allows one to describe the motion of the wake and account for its influence on the fluid loads. The novelty of this effort lies in a modification of the predicted forces as a generalisation of the leading edge suction analogy. This modification is introduced to account for the delayed stall mechanism due to leading edge flow separation. The model predictions are compared with two sets of independent experimental data and with computational fluid dynamics (CFD) simulation data available in the literature. It is found that both, the modified analytical model and the UVLM model can be used to describe the time history of the lift force, in some cases with better results than a high-fidelity CFD model. The models presented here constitute a useful basis for the aerodynamic design of bioinspired flapping-wings micro-air vehicles.
The second season of excavations at Jebel Moya (south-central Sudan)
Authors: Brass Michael , Adam Ahmed , Vella Gregory Isabelle , Abdallah Rayan , Alawad Osman , Abdalla Ammar , Kozieradzka-Ogunmakin Iwona , Wellings Joss , Albadwi Anfal , Moyne Charles Le , Hassan Fakri , Abdelrahman Ali 
Published: July 2020

AbstractThis report presents the latest data from ongoing excavations at Jebel Moya, Sudan. This year saw the opening of five new trenches and continued excavation of an archaeologically rich trench. We have recovered four individual burials, a mud brick wall and a number of animal and archaeobotanical remains. The excavations also yielded a longer pottery sequence, showing clearly that the site was in use by at least the sixth millennium BC. This season confirms the long and complex history of Jebel Moya and provides the material for future studies on population health and subsistence. This season also saw an increase in community engagement and a more detailed study of the various historical trajectories that make up the biography of Jebel Moya.
Autism in Aotearoa
Authors: Kember Sarah M. , Williams Matt N. 
Published: July 2020

Abstract. Screening measures for autism spectrum disorder (ASD) are important tools for clinicians and researchers. However, where a measure developed and validated for one population is used with another, its performance in this new context must be carefully examined. The RAADS-14, a brief ASD screen developed in Sweden, was evaluated with a sample of New Zealand adults ( N = 387), 41 of whom self-reported a prior diagnosis of ASD. The convergent validity of the RAADS-14 (Hypothesis 2) was supported by a strong positive correlation with the AQ-10 (short version of the Autism Spectrum Quotient), r = .81. Discriminant validity (Hypothesis 3) was also supported by a strong negative correlation with the EQ-Short (short version of the Empathy Quotient), r = −.75. However, the measure did not meet inferential criteria for internal consistency (Hypothesis 1), and confirmatory factor analysis (CFA) found a poor fit of the proposed three-factor model (Hypothesis 4) to the data. A cut-off score of 14/42 provided adequate sensitivity (95%) to detect participants with self-reported ASD diagnoses, but not adequate specificity (70%), suggesting a very high rate of false positives should be expected if relying on RAADS-14 scores alone to interpret presence of ASD. In sum, our results do not provide sufficient evidence of reliability and validity to support the use of the RAADS-14 with the New Zealand population. We provide suggestions for refinement of the RAADS-14 that may lead to increased reliability and validity.
Importance of Boundary Processes for Heat Uptake in the Subpolar North Atlantic
Authors: Desbruyères D. G. , Sinha B. , McDonagh E. L. , Josey S. A. , Holliday N. P. , Smeed D. A. , New A. L. , Megann A. , Moat B. I. 
Published: July 2020

AbstractThe decadal to multidecadal temperature variability of the intermediate (700–2,000 m) North Atlantic Subpolar Gyre (SPG) significantly imprints the global pattern of ocean heat uptake. Here, the origins and dominant pathways of this variability are investigated with an ocean analysis product (EN4), an ocean state estimate (ECCOv4), and idealized modeling approaches. Sustained increases and decreases of intermediate temperature in the SPG correlate with long‐lasting warm and cold states of the upper ocean with the largest anomalous vertical heat exchanges confined to the vicinity of continental boundaries and strong ocean currents. In particular, vertical diffusive processes along the boundaries of the Labrador, Irminger, and Newfoundland basins are important drivers of the recent intermediate depth warming trend observed during 1996–2014. The overall effect of those processes is captured by a one‐dimensional diffusive model with appropriate boundary‐like parametrization and demonstrated through the boundary‐focused downward propagation of a passive tracer in a 3‐D numerical simulation. Our results imply that the slow and quasi‐periodic ventilation of intermediate thermohaline properties and associated heat uptake in the SPG are not strictly driven by convection‐restratification events in the open seas but also receives a key contribution from boundary sinking and mixing. Increased skill for modeling and predicting intermediate‐depth ocean properties in the North Atlantic will hence require the appropriate representation of surface‐deep dynamical connections within the boundary currents encircling Greenland and Newfoundland.
Impact of Convectively Detrained Ice Crystals on the Humidity of the Tropical Tropopause Layer in Boreal Winter
Authors: Ueyama R. , Jensen E. J. , Pfister L. , Krämer M. , Afchine A. , Schoeberl M. 
Published: July 2020

AbstractDeep convection detraining in the uppermost tropical troposphere is capable of transporting water vapor and ice into the tropical tropopause layer (TTL), but the impact of deep convection on the global and regional TTL water vapor budget remains uncertain. In particular, the role of convectively detrained ice crystals that remain suspended after active convection has subsided is not well understood. These ice crystals represent aging cirrus anvils detached from the convective core. We use a cloud microphysical model that tracks individual ice crystals throughout their lifetimes to quantify the impact of detrained ice on the humidity of the TTL during boreal winter. Convective influence of air parcels near the wintertime cold point tropical tropopause is determined by tracing thousands of backward trajectories through satellite‐derived, global, 3‐hourly convective cloud‐top altitude fields. Detrained ice, most of which is found over the tropical western Pacific, experiences cooling on the order of 1 K day−1 downstream of convection. Downstream cooling increases relative humidity and explains the observed supersaturated TTL over this region. Vapor in excess of saturation condenses onto the detrained ice, which ultimately brings the relative humidity down to saturation. Thus, convectively detrained ice crystals in aging anvils predominantly dehydrate the TTL, but the effect is small (0.01 ppmv). Moistening by active convection (0.30 ppmv), including the rapid sublimation of convectively lofted ice crystals near the tops of core anvils, overwhelms the dehydration by aging anvil ice crystals detrained from the core. The net effect is moistening by convective core anvils during boreal winter.
Structural heterogeneity of the mammalian polycomb repressor complex in immune regulation
Authors: Kang Seok-Jin , Chun Taehoon 
Published: July 2020

AbstractEpigenetic regulation is mainly mediated by enzymes that can modify the structure of chromatin by altering the structure of DNA or histones. Proteins involved in epigenetic processes have been identified to study the detailed molecular mechanisms involved in the regulation of specific mRNA expression. Evolutionarily well-conserved polycomb group (PcG) proteins can function as transcriptional repressors by the trimethylation of histone H3 at the lysine 27 residue (H3K27me3) and the monoubiquitination of histone H2A at the lysine 119 residue (H2AK119ub). PcG proteins form two functionally distinct protein complexes: polycomb repressor complex 1 (PRC1) and PRC2. In mammals, the structural heterogeneity of each PRC complex is dramatically increased by several paralogs of its subunit proteins. Genetic studies with transgenic mice along with RNA-seq and chromatin immunoprecipitation (ChIP)-seq analyses might be helpful for defining the cell-specific functions of paralogs of PcG proteins. Here, we summarize current knowledge about the immune regulatory role of PcG proteins related to the compositional diversity of each PRC complex and introduce therapeutic drugs that target PcG proteins in hematopoietic malignancy.
A user guide for the online exploration and visualization of PCAWG data
Authors: Goldman Mary J. , Zhang Junjun , Fonseca Nuno A. , Cortés-Ciriano Isidro , Xiang Qian , Craft Brian , Piñeiro-Yáñez Elena , O’Connor Brian D. , Bazant Wojciech , Barrera Elisabet , Muñoz-Pomer Alfonso , Petryszak Robert , Füllgrabe Anja , Al-Shahrour Fatima , Keays Maria , Haussler David , Weinstein John N. , Huber Wolfgang , Valencia Alfonso , Park Peter J. , Papatheodorou Irene , Zhu Jingchun , Ferretti Vincent , Vazquez Miguel 
Published: July 2020

AbstractThe Pan-Cancer Analysis of Whole Genomes (PCAWG) project generated a vast amount of whole-genome cancer sequencing resource data. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2658 cancers across 38 tumor types, we provide a user’s guide to the five publicly available online data exploration and visualization tools introduced in the PCAWG marker paper. These tools are ICGC Data Portal, UCSC Xena, Chromothripsis Explorer, Expression Atlas, and PCAWG-Scout. We detail use cases and analyses for each tool, show how they incorporate outside resources from the larger genomics ecosystem, and demonstrate how the tools can be used together to understand the biology of cancers more deeply. Together, the tools enable researchers to query the complex genomic PCAWG data dynamically and integrate external information, enabling and enhancing interpretation.
Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)
Authors: Zwettler Fabian U. , Reinhard Sebastian , Gambarotto Davide , Bell Toby D. M. , Hamel Virginie , Guichard Paul , Sauer Markus 
Published: July 2020

AbstractExpansion microscopy (ExM) enables super-resolution fluorescence imaging of physically expanded biological samples with conventional microscopes. By combining ExM with single-molecule localization microscopy (SMLM) it is potentially possible to approach the resolution of electron microscopy. However, current attempts to combine both methods remained challenging because of protein and fluorophore loss during digestion or denaturation, gelation, and the incompatibility of expanded polyelectrolyte hydrogels with photoswitching buffers. Here we show that re-embedding of expanded hydrogels enables dSTORM imaging of expanded samples and demonstrate that post-labeling ExM resolves the current limitations of super-resolution microscopy. Using microtubules as a reference structure and centrioles, we demonstrate that post-labeling Ex-SMLM preserves ultrastructural details, improves the labeling efficiency and reduces the positional error arising from linking fluorophores into the gel thus paving the way for super-resolution imaging of immunolabeled endogenous proteins with true molecular resolution.
Spiking neurons with spatiotemporal dynamics and gain modulation for monolithically integrated memristive neural networks
Authors: Duan Qingxi , Jing Zhaokun , Zou Xiaolong , Wang Yanghao , Yang Ke , Zhang Teng , Wu Si , Huang Ru , Yang Yuchao 
Published: July 2020

AbstractAs a key building block of biological cortex, neurons are powerful information processing units and can achieve highly complex nonlinear computations even in individual cells. Hardware implementation of artificial neurons with similar capability is of great significance for the construction of intelligent, neuromorphic systems. Here, we demonstrate an artificial neuron based on NbOx volatile memristor that not only realizes traditional all-or-nothing, threshold-driven spiking and spatiotemporal integration, but also enables dynamic logic including XOR function that is not linearly separable and multiplicative gain modulation among different dendritic inputs, therefore surpassing neuronal functions described by a simple point neuron model. A monolithically integrated 4 × 4 fully memristive neural network consisting of volatile NbOx memristor based neurons and nonvolatile TaOx memristor based synapses in a single crossbar array is experimentally demonstrated, showing capability in pattern recognition through online learning using a simplified δ-rule and coincidence detection, which paves the way for bio-inspired intelligent systems.
Structure of the yeast Swi/Snf complex in a nucleosome free state
Authors: Wang Chengcheng , Guo Zhouyan , Zhan Xiechao , Yang Fenghua , Wu Mingxuan , Zhang Xiaofeng 
Published: July 2020

AbstractSWI/SNF remodelers play a key role in regulating chromatin architecture and gene expression. Here, we report the cryo-EM structure of the Saccharomyces cerevisiae Swi/Snf complex in a nucleosome-free state. The structure consists of a stable triangular base module and a flexible Arp module. The conserved subunits Swi1 and Swi3 form the backbone of the complex and closely interact with other components. Snf6, which is specific for yeast Swi/Snf complex, stabilizes the binding of the ATPase-containing subunit Snf2 to the base module. Comparison of the yeast Swi/Snf and RSC complexes reveals conserved structural features that govern the assembly and function of these two subfamilies of chromatin remodelers. Our findings complement those from recent structures of the yeast and human chromatin remodelers and provide further insights into the assembly and function of the SWI/SNF remodelers.
A self-organizing, living library of time-series data
Authors: Fulcher Ben D. , Lubba Carl H. , Sethi Sarab S. , Jones Nick S. 
Published: July 2020

AbstractTime-series data are measured across the sciences, from astronomy to biomedicine, but meaningful cross-disciplinary interactions are limited by the challenge of identifying fruitful connections. Here we introduce the web platform,CompEngine, a self-organizing, living library of time-series data, that lowers the barrier to forming meaningful interdisciplinary connections between time series. Using a canonical feature-based representation,CompEngineplaces all time series in a common feature space, regardless of their origin, allowing users to upload their data and immediately explore diverse data with similar properties, and be alerted when similar data is uploaded in future. In contrast to conventional databases which are organized by assigned metadata,CompEngineincentivizes data sharing by automatically connecting experimental and theoretical scientists across disciplines based on the empirical structure of the data they measure.CompEngine’s growing library of interdisciplinary time-series data also enables the comprehensive characterization of time-series analysis algorithms across diverse types of empirical data.
Phenotypic plasticity in diaspore production of a amphi-basicarpic cold desert annual that produces polymorphic diaspores
Authors: Gan Lu , Lu Juanjuan , Baskin Jerry M. , Baskin Carol C. , Tan Dunyan 
Published: July 2020

AbstractPhenotypic plasticity has been studied in diaspore-dimorphic species, but no such study has been done on a diaspore-polymorphic species. Our aim was to determine the effects of abiotic and biotic factors on phenotypic plasticity of the diaspore-polymorphic cold desert annual Ceratocarpus arenarius. Plants produced from dispersal units near the soil surface (a, basicarps) and at the middle (c) and upper (f) parts of the plant canopy were subjected to different levels of soil moisture, nutrient supply and intramorph and intermorph densities. Different levels of these biotic and abiotic factors resulted in significant variation in total plant mass, diaspore mass, mass allocation to stem and reproductive organs and total number and proportion of morphs a, c and f on an individual. The effect of stress on number and mass of a dispersal unit morph varied by treatment, with dispersal unit f having the highest CV and dispersal unit a the lowest. The success of this diaspore polymorphic species in its rainfall-unpredictable environment likely is enhanced by plasticity in production of the different types of diaspores.