bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early Embryo Development in Women with Endometriosis: A Retrospective Cohort Study
Authors: Hamdan Mukhri B. , Cotterill M , Price A , Cheong Y 
Published: June 2020

Abstract
Background: Endometriosis is known to be detrimental to fertility in many ways. Evidence drawn from studies on oocyte recipient cycles suggests that the oocyte quality of women with endometriosis is compromised. Research that specifically examined the association between embryo quality and endometriosis/endometrioma are scarce and the question if endometriosis affects oocyte and/or embryo quality remain controversial. This study aims to evaluate embryo quality of women with endometriosis (EN) and without endometriosis (tubal factor [TF], unexplained infertility [UE]).Methods: Multi-centre retrospective study was performed. Treatment cycles and embryology records of women with and without endometriosis undergoing IVF was reviewed via IDEASTM database. Number of oocytes collected per mature follicle, fertilisation rate, pregnancy rate, morphology and morphometric assessment of embryo at all stages were evaluatedResults: Total of 678 women who had IVF treatment was analysed (EN, n=89; TF, n=214; UE, n=375). The mean age for each group was EN, 34.4±3.3; TF, 33.5±3.7 and 34.6±3.2 respectively. Number of mature follicles (>14mm) on the day of trigger injection was similar (P=0.75) between all groups. Percentage of oocytes collected per mature follicle was lower in endometriosis compared to other groups (P=0.01; EN, 65±23; TF, 76±20; UE, 71±24). Higher percentages of embryos fail to achieve 8-cell stage in EN compared to control groups (P=0.02; EN, 4.0±1.6 TF, 1.2±0.4; UE, 1.5±0.4. Percentages of embryos at all grades (Grade1-4) per women were similar between the comparison groups (P>0.05). Endometriosis did not impair (P>0.05) blastocyst development or the development of Fully Expanded Hatching Blastocysts (FEHB).Conclusions: This study finds women with endometriosis have lower number of oocyte per total number of follicle. The presence of endometriosis is associated with a higher rate of early embryo arrest, which implicates poor oocyte quality. However endometriosis has no effect on the embryo quality beyond the 8-cell-stage of embryo development
The predictive value of low urine specific gravity in the diagnosis of vasovagal syncope in children and adolescents
Authors: Liu Ping , Zeng Xingfang , Yang Hong , Mei Wanzhen , Wang Yuwen , Zhang Juan , Zou Runmei , Wang Cheng 
Published: June 2020

Abstract
Background
Vasovagal syncope (VVS) is a kind of common neurogenic syncope in children and adolescents. Decreased blood volume is one of the pathogenesis of VVS. The study will explore the predictive value of low urine specific gravity (USG) in the diagnosis of VVS in children and adolescents.
Methods
97 cases, 43 males and 54 females, aged 4 to 16 years old, with an average age of 10.91 ± 2.18 years old, due to unexplained premonitory syncope or syncope were selected. They were diagnosed with VVS through head-up tilt table test. During the same period, 91 cases of children and adolescents, including 45 males and 46 females, aged from 5 to 15 years old, who underwent a healthy examination were matched as a control (control group). USG was measured in both groups.
Results
The USG of VVS group was significantly lower than that of the control group (P < 0.01). The sensitivity and specificity of USG prediction in the diagnosis of VVS were evaluated by ROC curve. The area under the ROC curve was 0.751, standard error was 0.035, and 95% CI (0.683, 0.819) suggested that USG was of moderate predictive value in the diagnosis of VVS. Setting USG as < 1.0185, the sensitivity and specificity and diagnostic coincidence rate of VVS were 74.39%, 66.04% and 69.68%, respectively.
Conclusion
Low USG has predictive value in the diagnosis of VVS in children and adolescents.
Biochar addition alleviate the negative effects of drought and salinity stress on soybean productivity and water use efficiency
Authors: Zhang Yaojun , Ding Jiaqi , Wang Hong , Su Lei , Zhao Cancan 
Published: June 2020

Abstract
Background: Environmental stress is a crucial factor restricting plant growth as well as crop productivity, thus influencing the agricultural sustainability. Biochar addition is proposed as an effective management to improve crop performance. However, there were few studies focused on the effect of biochar addition on crop growth and productivity under interactive effect of abiotic stress (e.g., drought and salinity). This study was conducted with a pot experiment to investigate the interaction effects of drought and salinity stress on soybean yield, leaf gaseous exchange and water use efficiency (WUE) under biochar addition. Results: Drought and salinity stress significantly depressed soybean phenology (e.g. flowering time) and all the leaf gas exchange parameters, but had inconsistent effects on soybean root growth and WUE at leaf and yield levels. Salinity stress significantly decreased photosynthetic rate, stomatal conductance, intercellular CO2 concentration and transpiration rate by 20.7%, 26.3%, 10.5% and 27.2%, respectively. Lower biomass production and grain yield were probably due to the restrained photosynthesis under drought and salinity stress. Biochar addition significantly enhanced soybean grain yield by 3.1-14.8%. Drought stress and biochar addition significantly increased WUE-yield by 27.5% and 15.6%, respectively, while salinity stress significantly decreased WUE-yield by 24.2%. Drought and salinity stress showed some negative interactions on soybean productivity and leaf gaseous exchange. But biochar addition alleviate the negative effects on soybean productivity and water use efficiency under drought and salinity stress. Conclusions: The results of the present study indicated that drought and salinity stress could significantly depress soybean growth and productivity. There exist interactive effects of drought and salinity stress on soybean productivity and water use efficiency, while we could employ biochar to alleviate the negative effects. We should consider the interactive effects of different abiotic restriction factors on crop growth thus for sustainable agriculture in the future.
Zinc As A Factor Affecting Serum Calcification Propensity in Patients With Type 2 Diabetes Mellitus
Authors: Nakatani Shinya , Mori Katsuhito , Sonoda Mika , Nishide Kozo , Uedono Hideki , Tsuda Akihiro , Emoto Masanori , Shoji Testuo 
Published: June 2020

Abstract
Background: Zinc inhibits vascular calcification in vivo and in vitro. Patients with type 2 diabetes mellitus show hypozincemia and are at an elevated risk of cardiovascular events. Recently, the in vitro test (T50-test) was developed for the determination of serum calcification propensity. This cross-sectional study investigated the association between serum zinc and T50 in type 2 diabetes mellitus patients and the effect of zinc on T50in vitro.Methods: The subjects were 132 type 2 diabetes mellitus patients with various kidney function. We measured T50 levels by the established nephelometric method.Results: The median (interquartile range) levels of T50 and serum zinc were 306 (269 to 332) min, and 80.0 (70.1 to 89.8) µg/dL, respectively. Serum zinc level was significantly and positively correlated with T50 (rs = 0.219, p = 0.012). This association remained significant in multivariable-adjusted analysis, and was independent of known factors including phosphate, calcium, and magnesium. Renal function and glycemic control were not significantly associated with T50. Finally, addition of physiological concentration of exogenous zinc chloride significantly increased the serum T50 in vitro.Conclusions: This is the first report to investigate the association between serum calcification propensity and zinc levels in type 2 diabetes mellitus patients. Our data suggest that serum zinc is an independent factor that inhibits serum calcification propensity.
LincHOXA10 Facilitates Colorectal Cancer Development By Regulating HOXA10-Mediated Epithelial-Mesenchymal Transtion
Authors: Zhu Huifang , Li Yongzhen , Zhang Yinghui , Zhang Zheying , Wang Yongxia , He Guoyang , Li Na , Wang Haijun , Zhong Jiateng , Qian Xinlai , Hu Yuhan 
Published: June 2020

Abstract
Background: Long non-coding RNAs (lncRNAs) have been reported to play an important role in tumorigenesis and metastasis of human colorectal cancer (CRC). However, the specific role of LincHOXA10 in CRC remains unknown.Methods: The expression of LincHOXA10 and HOXA10 in CRC cells and tissue samples was measured by quantitative reverse transcription PCR (qRT-PCR). The protein expression of HOXA10, E-cadherin, N-cadherin, Vinmentin, p-smad2 and p-smad3 was assessed by Western blotting or immunofluorescence staining. Cell proliferation, migration, and invasion were assessed by the MTT and transwell assays. Tumor growth in vivo was carried out by subcutaneous tumor formation in nude mice.Results: In the present study, we found that LincHOXA10 expression was significantly higher in human CRC tissues than the paired normal tissues. In fact, LincHOXA10 level correlated with the CRC tumor sizes and lymphatic metastasis. In cultured CRC cells, knockdown of LincHOXA10 inhibited cell proliferation, migration and invasion. LincHOXA10 deficiency also attenuated CRC tumor growth in vivo. Mechanistically, LincHOXA10 interacted with HOXA10 and regulated its expression. HOXA10 levels were interrelated to the LincHOXA10 level in CRC cells. Functionally, HOXA10 was essential for TGF-β1/SMADs-induced epithelial -mesenchymal transition of CRC cells, and HOXA10 played a critical role in mediating the function of LincHOXA10. Importantly, HOXA10 expression was significantly up-regulated in human CRC tissues.Conclusions: LincHOXA10 facilitates CRC development and metastasis via regulating HOXA10-mediated epithelial-mesenchymal transition of CRC cells.
Pulsed Shortwave Therapy in Cervical Osteoarthritis: An NSAID- Controlled, Randomized Clinical Trial
Authors: Mohameed Rachid El , Koneru Sree , Staelin Richard , McLeod Kenneth , Tabbouche Omar , Rawe Ian 
Published: June 2020

Abstract
Objective Assess treatment superiority of Pulsed Shortwave Therapy (PSWT) against COX-2 NSAID therapy, in reducing disability and pain due to cervical osteoarthritis.Design 200chronic pain suffers (average pain duration about 2 years)diagnosed with cervical osteoarthritis by radiological imaging were randomized into one of two treatmentarms: COX-2 NSAID treatment: Etoricoxib 60mg/day for 4 weeks; or, PSWT treatmentworn 24 hours/day for 4 weeks. The primary outcome measure was the 4-week score on the Neck Disability Index (NDI): a 10-question assessment on a 50-point scale. Secondary outcome measures included pain(at rest and during activity)measured on a Visual Analog Scale (VAS)of0-100 mm, dose count of rescue pain medication (paracetamol)use and a treatment satisfaction rating. These 4-week scores were compared across the two arms to assess superiority.Results After 4 weeks of treatment,subjects in both study arms reported significantly lower (p<0.0001) 4-week measures (11.24-NSAID;9.34-PSWT; 0-50 points),VASrest (30.08-NSAID;22.76-PSWT; 0-100 mm) and VASactivity (36.40-NSAID; 27.42-PSWT; 0-100 mm).The absolute reduction from baseline in NDI was significantly greater in the PSWT arm than NSAID arm(by 3.66 points; 95% CI 2.3 to 5.02; p<0.0001). Similarly, the reductions from baseline inVASrest and VASactivitywere significantly greater in the PSWT arm than NSAID arm(by 10.89 mm;95% CI 6.90 to 14.87; p<0.0001 and 12.05 mm;95% CI 7.76 to 16.33; p<0.0001 respectively).The PSWT arm used 50% less rescue pain medication. Eleven adverse effects were reported in the NSAID arm and zero in the PSWT arm.Conclusion Both NSAID and PSWT treatments resulted in clinically meaningful increases in quality of life(NDI) and decreases in pain (VAS) associated with cervicalosteoarthritis. However, the PSWT armshowed superior improvements in all outcome measures when compared to the NSAID armwith no adverse effects.
Mapping the Multidimensionality of Medical Care related Catastrophe on Households: A Study of Four Blocks in Birbhum District, West Bengal
Authors: Dasgupta Priyanka , Mukherjee Subrata 
Published: June 2020

Abstract
Background

Medical care related catastrophe is generally identified by healthcare expenses crossing a certain percentage of household’s resources (Wagstaff and van Doorslaer, 2003). This paper attempts to examine medical care related ‘catastrophe’ by going beyond the threshold-oriented approach of catastrophic medical expenditure and include multiple indicators which seeks to explore the catastrophe from a multidimensional perspective.
Methods

Drawing from multidimensional vulnerability to poverty approach (Alkire and Forster, 2008), we provide a measure which incorporates multiple indicators that might put households in medical care related catastrophic situation. Our study uses data from a cross-sectional household survey conducted by the Society for Health and Demographic Surveillance (SHDS) of the Government of West Bengal in 2012. Using negative binomial and logistic regression, the study also attempts to find the correlates of healthcare utilization, incurring catastrophic health expenditure for both 10 per cent of household consumption expenditure and 40 per cent of non-food expenditure as well as resorting to distress financing and availing low quality/ no care despite chronic illness.
Results

Estimates show that illness, presence of elderly members, hospitalization and outpatient visits increases the risk of incurring catastrophic health expenditure and healthcare utilization counts. In addition, households belonging to backward socio-religious categories and having members engaged as casual labourers face higher odds of distressed financing and availing informal/ no healthcare. In contrast to Wagstaff and van Doorslaer’s measure of catastrophic medical expenses, the multidimensional measure shows a lower medical care catastrophe for the upper economic classes and forward caste groups and vice versa which is more realistic and convincing.
Conclusions

The evidence generated from the multidimensional analysis presents a more convincing and reliable picture of vulnerability imparted due to health shocks as compared to identifying households with catastrophic medical expenditure by Wagstaff and van Doorslaer’s method. However, this study has its limitations as it has given equal weightage to all the dimensions and restricted itself to headcount measures. However, extension and refinement of this approach can provide more insightful findings.
Is Coracoclavicular Reconstruction Necessary in Hook Plate Fixation for Acute Unstable Acromioclavicular Dislocation?
Authors: Chen Yu-Ta , Wu Kuan-Ting , Jhan Shun-Wun , Hsu Shan-Lin , Liu Hao-Chen , Wang Ching-Jen , Ko Jih-Yang , Chou Wen-Yi 
Published: June 2020

Abstract
Background: Acromioclavicular joint (ACJ) dislocation is a relatively common shoulder injury. For the treatment of cases of severe ACJ dislocation (Rockwood type III–V), hook plate fixation is an easy-to-master and minimally-invasive approach to surgical intervention. Overstress on the acromion following hook plate fixation often leads to acromial complications such as osteolysis and loss of reduction. We hypothesized that suspensory reconstruction alongside hook plate fixation might provide a superior stability and reduce complications as compared with hook plate fixation alone. The purpose of the study was to assess the clinical and radiographic outcomes of these two surgical modalities.Methods: We retrospectively enrolled 38 patients with acute ACJ dislocation from May 2010 to December 2018. Among them, nineteen patients received hook plate fixation only (HP group), and 19 underwent concomitant hook plate fixation and loop suspension fixation with two mersilene tapes (HM group). The demographic data of the patients were recorded and analyzed. All patients underwent a shoulder x-ray initially, immediately postoperatively, and at 1, 3, 6 and 12 months to measure the relative coracoclavicular distance (rCCD). Clinical assessment of shoulder function outcome was conducted using the Constant Murley Score (CMS); the University of California at Los Angeles (UCLA) Shoulder Score was also measured at the latest follow-up.Results: There were no significant differences in the demographic data between the two groups. With regards to the CMS and the UCLA score, the HM group and HP group both had excellent outcomes, and no significant differences in scores were observed between groups (CMS: 93.90 ± 6.16 versus 94.47 ± 7.26, p = 0.47; UCLA score: 32.84 ± 2.91 versus 34.32 ± 1.16, p = 0.07). However, the HM group demonstrated substantial superiority in terms of maintenance of the rCCD over the HP group (91.47 ± 27.47 versus 100.75 ± 48.70, p = 0.015). In addition, there was less subacromial osteolysis in the HM group than the HP group (52.6% versus 15.8%, p = 0.038).Conclusion: Concomitant hook plate fixation with loop suspensory reconstruction yielded excellent functional outcomes and superior maintenance of the rCCD with fewer acromion complications than hook plate fixation alone.
Superior Articular Process Osteotomy Navigation (SapON) Technology in Percutaneous Endoscopic Lumbar Discectomy: A New Surgical Technology
Authors: Li Jianhui , Ma Boyuan , Wang Mengyao , Li Jianwei , Tian Shuijing , Sun Chuan , Shi Guidong , Zhou Xianhu 
Published: June 2020

Abstract
Background: This research aims to discuss the clinical application value of the superior articular process osteotomy navigation (SapON) technique in percutaneous endoscopic lumbar discectomy.Methods: Patients who underwent intervertebral foraminal endoscopic surgery for degenerative disease of the lumbar spine were collected and divided into two groups from March 2016 to May 2018. Group I: 69 patients were treated by the SapON technique. Group II: 53 patients were treated by Transforaminal Endoscopic Spine Surgery (TESS) technique. Fluoroscopy times, the time of establishment working channel, endoscopic operations, and entire operations in two groups were recorded. Patients' clinical curative effect was evaluated by visual analog scale (VAS) scores, Oswestry disability index(ODI), and MacNab standard at different time points, including before surgeries, one day, three months after surgeries, and the last follow-up time.Results: Operations were all successful both in groups I and II. Fluoroscopy times, establishing the working tunnels time, endoscopic operations time, and entire operations time and other indexes between the two groups were statistically significant. Patients’ low back, legs pain VAS scores, and ODI one day, three months after surgeries, and at the last follow-up in both two groups were significantly lower than those before operations with a statistical difference. The excellent rate in groups I and II were 98.55% and 96.23%. Complications were not observed in group I, while in group II, only one patient suffered the injury of the right L5 nerve root, and recovered after three months’ conservative treatment.Conclusion: The application of SapON in percutaneous endoscopic lumbar discectomy (PELD) has its advantages to popularize in clinical work, including operating simply and orderly, high safety, fewer complications, lower radiation exposure, and good clinical effects
Pseudogene KRT17P3 Drives Cisplatin Resistance of Human NSCLC Cells by Modulating miR-497-5p/mTOR
Authors: Hou Zhibo , Zhou Qianwen , Xia Ning , Lv Tang-feng , Yuan Xiaoqin , Song Yong 
Published: June 2020

Abstract
Background: Chemoresistance is a major obstacle in non-small-cell lung cancer (NSCLC) treatment. The pseudogene KRT17P3 has been shown to be up-regulated in lung cancer tissues from patients with cisplatin resistance. However, its molecular mechanism in promoting chemoresistance has not been elucidated.Methods: Real-time PCR was performed to evaluate KRT17P3 levels in plasma samples collected from 30 cisplatin-resistance patients and 32 cisplatin-sensitive patients. KRT17P3 was overexpressed or knocked down in A549 and SK-MES-1 NSCLC cells to evaluate the effects of KRT17P3 on the in vitro and in vivo resistance of NSCLC to cisplatin. Cell Counting Kit 8, Annexin V/propidium iodide staining, and PARP-1 cleavage assays were performed to evaluate cell viability and apoptosis. Dual luciferase reporter, RNA immunoprecipitation, Western blot assays, and rescue experiments were used to evaluate the functional interaction of KRT17P3, miR-497-5p and mTOR.Results: Our results demonstrate that KRT17P3 overexpression in cultured NSCLC cells increases cell viability and decreases apoptosis upon cisplatin treatment, while KRT17P3 knockdown has the opposite effect. KRT17P3 overexpression promotes NSCLC tumor growth upon in cisplatin-treated xenografted mice. Mechanistically, KRT17P3 acts as a molecular sponge for miR-497-5p and relieves the binding of miR-497-5p to its target gene mTOR. Rescue experiments validated the functional interaction among KRT17P3, miR497-5p, and mTOR. Moreover, the plasma level of KRT17P3 is up-regulated in cisplatin-resistance patients.Conclusions: Our findings indicate that KRT17P3/miR497-5p/mTOR regulates the chemosensitivity of NSCLC, suggesting a potential therapeutic target for cisplatin-resistant NSCLC patients. KRT17P3 may be a potential peripheral blood marker of NSCLC patients resistant to cisplatin.
Analysis of Biliary Microbiota in Hepatobiliopancreatic Diseases Compared to Healthy People [MICROBILIO]: Study Protocol
Authors: Nascimento Fernanda Sayuri do , Suzuki Milena Oliveira , Taba João Victor , de Mattos Vitoria Carneiro , Pipek Leonardo Zumerkorn , D’Albuquerque Eugênia Machado Carneiro , Meyer Alberto , Andraus Wellington , Pinho João Renato Rebello , de Moura Eduardo Guimarães Hourneaux , Setubal João Carlos , Carneiro-D’Albuquerque Luiz Augusto 
Published: June 2020

Abstract
Background: The performance of the microbiota is observed in several digestive tract diseases. Therefore, reaching the biliary microbiota may suggest ways for studies of biomarkers, diagnoses, tests and therapies in hepatobiliopancreatic diseases.Methods: Bile samples will be collected in endoscopic retrograde cholangiopancreatography patients (case group) and living liver transplantation donors (control group). From the DNA extracted from the samples, the microbiomes will be sequenced by 16S ribosomal RNA (rRNA) pyrosequencing methods. Student’s t-test will be performed with SPSS version 20 for Windows and the Mann-Whitney test will be performed using R software and Python scripts. A principal component analysis signals will be developed as discriminative analysis.Results: The primary expected results of the study is to characterize the specific composition of the biliary microbiota in situations of disease and health. In addition, it seeks to demonstrate the existence of changes in the case of illness and also possible disease biomarkers, diagnosis, interventions and therapies in hepatobiliopancreatic diseases.Conclusions: We believe that the investigation of the biliary tract microbiota will provide benefits such as early diagnosis and new treatments. This should improve quality of life and survival, specially in malignant diseases. Thus, this research will give expectations to further investigation in the biliary microbiota.Trial registration: NCT04391426. Registered 18 May 2020, https://clinicaltrials.gov/ct2/show/NCT04391426