bluuebunny/crossref_metadata_2025_split huggingface dataset:
SOX9 Is Essential for Triple-Negative Breast Cancer Cell Survival and Metastasis
Authors: Ma Yanxia , Shepherd Jonathan , Zhao Dekuang , Bollu Lakshmi Reddy , Tahaney William M. , Hill Jamal , Zhang Yun , Mazumdar Abhijit , Brown Powel H. 
Published: July 2020

Abstract

Triple-negative breast cancer (TNBC) has the worst prognosis of all breast cancers, and lacks effective targeted treatment strategies. Previously, we identified 33 transcription factors highly expressed in TNBC. Here, we focused on six sex determining region Y-related HMG-box (SOX) transcription factors (SOX4, 6, 8, 9, 10, and 11) highly expressed in TNBCs. Our siRNA screening assay demonstrated that SOX9 knockdown suppressed TNBC cell growth and invasion in vitro. Thus, we hypothesized that SOX9 is an important regulator of breast cancer survival and metastasis, and demonstrated that knockout of SOX9 reduced breast tumor growth and lung metastasis in vivo. In addition, we found that loss of SOX9 induced profound apoptosis, with only a slight impairment of G1 to S progression within the cell cycle, and that SOX9 directly regulates genes controlling apoptosis. On the basis of published CHIP-seq data, we demonstrated that SOX9 binds to the promoter of apoptosis-regulating genes (tnfrsf1b, fadd, tnfrsf10a, tnfrsf10b, and ripk1), and represses their expression. SOX9 knockdown upregulates these genes, consistent with the induction of apoptosis. Analysis of available CHIP-seq data showed that SOX9 binds to the promoters of several epithelial–mesenchymal transition (EMT)- and metastasis-regulating genes. Using CHIP assays, we demonstrated that SOX9 directly binds the promoters of genes involved in EMT (vim, cldn1, ctnnb1, and zeb1) and that SOX9 knockdown suppresses the expression of these genes.

Implications:
Our studies identified the SOX9 protein as a “master regulator” of breast cancer cell survival and metastasis, and provide preclinical rationale to develop SOX9 inhibitors for the treatment of women with metastatic triple-negative breast cancer.
Efeito da ordem dos exercícios no treinamento de força sobre a pressão arterial pós esforço em idosas hipertensas
Authors: Cardozo Diogo , Vasconcelos Ana Paula , Figueiredo Tiago , Simão Roberto 
Published: July 2020

O objetivo deste estudo foi analisar o efeito de diferentes ordens de exercícios sobre a pressão arterial (PA) após sessões de treinamento de força (TF). Quinze idosas hipertensas inexperientes em TF foram divididas em dois grupos. Grupo de membros superiores (G1) e grupo membros inferiores (G2). O G1 realizou três séries de 15 repetições submáximas na sequência de exercícios: Supino Reto (SR), Remada Fechada (RF), Tríceps no Puxador (TP) e Rosca Bíceps (RB). A segunda sequência do G1 foi inversa (RB, TP, RF, SR). O G2 realizou quatro séries de 15 repetições submáximas na sequência de exercícios: Leg Press (LP), Cadeira Extensora (CE) e Flexão Plantar (FP). A segunda sequência do G2 foi inversa FP, CE, LP. A PA foi mensurada em repouso e após as sessões de exercícios durante 60 minutos. Para análise dos dados, utilizou-se uma ANOVA de dois caminhos com medidas repetidas e post hoc de Tukey para verificar as diferenças na PA antes e após as sessões de treinamento. Ambos os grupos apresentaram reduções significativas na PA sistólica pós-exercício com a sequência de exercícios do grande para o pequeno grupo muscular G1 (20, 30 e 40 min) e G2 (30, 40, 50 e 60 min). Quando a sequência foi inversa, foram observadas diferenças significativas emalguns momentos G1 (30 e 40 min) e G2 (40 e 50 min). Não foram encontradas diferenças significativas entre os grupos e também para a PA diastólica em qualquer sequência. Os resultados indicam que quando a sequência de exercícios é iniciada do grande para o pequeno grupo muscular, há uma tendência para maior duração do efeito hipotensivo.
Escala de avaliação da qualidade de vida por meio da Teoria da Resposta ao Item
Authors: Testa Wagner Luiz , Silveira Pablo Magno da , Borgatto Adriano Ferreti , Barbosa Aline Rodrigues 
Published: July 2020

O objetivo deste estudo foi criar uma escala para medir a qualidade de vida (QV) por meio da Teoria da Resposta ao Item (TRI). Para isso, foram coletados dados de 940 profissionais de Educação Física (PEF) registrados no Conselho Regional de Educação Física de Santa Catarina (CREF3-SC) em 2012, por meio do questionário WHOQOL-Bref. Para o tratamento dos dados, foi utilizado os recursos da TRI, com base no modelo de resposta gradual (MRG). Os resultados possibilitaram a criação de uma escala com 26 itens, com quatro categorias ordinais, em cinco níveis da escala. Na amostra dos PEF, os itens mais comuns na QV foram aqueles associados à dor física, à necessidade de tratamento médico e à capacidade de locomoção. Os itens mais incomuns foram aqueles associados às questões do ambiente físico, acesso aos serviços de saúde e questão financeira. O WHOQOL-Bref e o MRG mostraram-se adequados e forneceram recursos necessários à construção da escala de qualidade de vida.
Periodização ondulatória vs periodização linear
Authors: Fortes Leonardo De Sousa , Paes Pedro Pinheiro , Pérez Anselmo José , Paes Santiago Tavares , Diefenthaeler Fernando 
Published: July 2020

O objetivo da presente investigação foi analisar o efeito de dois diferentes tipos de periodização (ondulatória e linear) sobre o desempenho de nadadores. Trinta e nove jovens nadadores do sexo masculino foram selecionados de forma não probabilística e divididos proporcionalmente e randomicamente em dois grupos: periodização ondulatória (PO, n=19) e periodização linear (PL, n=20). Os nadadores foram submetidos a 8 semanas de treinamento físico/técnico. O desempenho nas provas de 50 e 100 m livre foi avaliado por meio de placas eletrônicas antes e após o período de treinamento. Conduziu-se a análise multivariada de medidas repetidas para comparar o desempenho (50 e 100 m livre) em função do grupo (PO e PL) e momento (pré e pós-treinamento). Foi identificado efeito de interação grupo vs. tempo (p<0,01) para o desempenho nos 50 m livre, com melhora no PO (∆%=-1,87; p=0,01; d=0,7) emanutenção no PL (∆%=-0,37; p=0,38; d=0,2). Achados similares foram revelados para os 100 m livre, com melhora no grupo PO (∆%=-1,85; p=0,01; d=0,9) e manutenção no grupo PL (∆%=-0,16; p=0,31; d=0,1). Concluiu-se que a periodização ondulatória foi mais eficiente para melhorar o desempenho de jovens nadadores quando comparada a periodização linear.
Percepção de estresse e estratégias de enfrentamento em atletas de futebol religiosos
Authors: Costa Cleberson Franclin Tavares , Oliveira Cristiane Costa da Cunha , Vargas Marlizete Maldonado 
Published: July 2020

O objetivo deste estudo foi analisar a percepção de atletas de futebol, religiosos e não religiosos, diante de situações estressoras e as estratégias de enfrentamento utilizadas. É um estudo do tipo survey, com 81 atletas de futebol das equipes semifinalistas do Campeonato Sergipano 2014. Utilizou-se o Inventário de Fatores de Stress no Futebol (ISF) e a Escala de Modos de Enfrentamento de Problemas (EMEP). Para o tratamento estatístico dos dados foram realizadas comparações de médias de respostas entre os grupos(religiosos x não religiosos) e aplicado o teste T Student. Por intermédio dos resultados, evidenciou-se que os atletas praticantes de alguma religião perceberam as situações estressoras mais negativamente, apesar de utilizarem estratégias de enfrentamento com maior frequência. Concluiu-se que a percepção de estressores, as estratégias de enfrentamento e a frequência em que essas são utilizadas apresentam diferenças entre os grupos, sendo recomendados novos estudos para comparativo e exploração da temática.
Wealth effects of dividend regulation: Evidence from China
Authors: Li Chao Kevin 
Published: July 2020

By exploring a natural experiment where the Chinese regulatory body introduced a dividend regulatory change in 2012, this article investigates the wealth effects of dividend regulation which increases firms’ dividends. I find that firms’ share price reacted positively (negatively) to regulatory events increasing (decreasing) investors’ expectation of dividends. The effects are more pronounced for firms with low dividends or domiciled in weak legal environments, robust to different research designs. The findings are consistent with the notion that low dividends in China are a manifestation of implicit contract failure. Dividend regulation provides remedies to low investor protection arising from weak legal environments. JEL Classification: G14, G35, G38, K22
The characteristics of an obesogenic small European country: results from a Malta cross-sectional study
Authors: Cuschieri S 
Published: July 2020

Aim: Obesity is a chronic disease and a global epidemic. The pathophysiology is well-documented; however, different countries are faced with different obesity characteristics. The aim was to establish the adult obesity characteristics of the obesogenic European country of Malta and explore for obesity links. Method: A national representative health examination survey was conducted. A single-stage random stratified sample population ( n = 4000) was obtained from a national register. Data on sociodemographic, comorbidities and lifestyle were collected. Body weight and height were measured, and blood collected for fasting glucose and lipid profile. Multiple binary logistic regression analyses through generalized linear models were performed to assess for associations between obesity, sociodemographic characteristics and dysglycaemic status. Results: Out of the study population ( n = 3947), 69.75% (confidence interval (CI) 95%: 68.32–71.18) were obese, with a male predominance (55%). The obese category sub-population resided mostly (25.80%, CI 95%: 23.53–28.21) within the highly densely populated district (Northern Harbour) of Malta and had a ‘routine and manual’ social position (55.76%, CI 95%: 53.10–58.40). On multiple logistic regression modelling, an obese status was linked with males (odds ratio (OR): 1.8, CI 95%: 1.53–2.12, p < .01), age (OR: 1.01, CI 95%: 1.01–1.02, p < .01), Northern Harbour district (OR: 1.11, CI 95%: 0.91–1.36, p < .02), type 2 diabetes (OR: 2.46, CI 95%: 1.79–3.38, p < .01) and impaired fasting blood glucose (IFG: 1.31, CI 95%: 1.17–1.90, p < .01) after adjusting for confounders. Conclusion: Obesity is a major health concern in Malta. Social determinants of health and metabolic changes appear to have a role in the obesity phenotype. Target actions that are gender-sensitive, social groups-focused and geographically oriented may be essential. The link between obesity and dysglycaemia brings forward the suggestion for incorporation of routine dysglycaemia screening during family doctors’ consultations for patients with higher than normal body weight.
Prognostic and clinicopathological significance of systemic immune-inflammation index in colorectal cancer: a meta-analysis
Authors: Dong Meilian , Shi Yonggang , Yang Jing , Zhou Quanbo , Lian Yugui , Wang Dan , Ma Taoran , Zhang Yue , Mi Yin , Gu Xiaobin , Fan Ruitai 
Published: July 2020

Background: Previous studies on the systemic immune-inflammation index (SII), which is based on platelet, neutrophil and lymphocyte counts, as a prognostic marker in patients with colorectal cancer (CRC) yielded inconsistent results. The aim of this study was to evaluate the prognostic and clinicopathological role of SII in CRC via meta-analysis. Methods: A comprehensive literature survey was performed on PubMed, Web of Science, Embase and the Cochrane Library databases to include studies published up to 6 April 2020. Pooled hazard ratios (HRs) and odds ratios (ORs) with 95% confidence intervals (CIs) were computed to estimate the prognostic and clinicopathological value of SII in CRC. Results: A total of 12 studies published between 2016 and 2019 were included in our meta-analysis. The combined analysis showed that high SII levels were significantly associated with worse overall survival (OS; HR = 1.61, 95% CI = 1.21–2.13, p = 0.001) and progression-free survival (HR = 1.74, 95% CI = 1.26–2.39, p = 0.001) in CRC. Moreover, elevated SII was also correlated with poor tumor differentiation (OR = 1.60, 95% CI = 1.27–2.02, p < 0.001), presence of distant metastasis (OR = 2.27, 95% CI = 1.10–4.67, p = 0.026), ECOG PS of 1–2 (OR = 1.98, 95% CI = 1.39–2.84, p < 0.001) and tumor size ⩾5 cm (OR = 1.49, 95% CI = 1.18–1.88, p = 0.001). However, high SII was not significantly associated with sex, tumor location, lymph node metastasis, or age in patients with CRC. Conclusion: Our meta-analysis indicated that high SII levels predicted poor prognosis in CRC. In addition, an elevated SII was also associated with clinical factors, implying higher malignancy of the disease.
Using phosphoproteomics data to understand cellular signaling: a comprehensive guide to bioinformatics resources
Authors: Savage Sara R. , Zhang Bing 
Published: July 2020

AbstractMass spectrometry-based phosphoproteomics is becoming an essential methodology for the study of global cellular signaling. Numerous bioinformatics resources are available to facilitate the translation of phosphopeptide identification and quantification results into novel biological and clinical insights, a critical step in phosphoproteomics data analysis. These resources include knowledge bases of kinases and phosphatases, phosphorylation sites, kinase inhibitors, and sequence variants affecting kinase function, and bioinformatics tools that can predict phosphorylation sites in addition to the kinase that phosphorylates them, infer kinase activity, and predict the effect of mutations on kinase signaling. However, these resources exist in silos and it is challenging to select among multiple resources with similar functions. Therefore, we put together a comprehensive collection of resources related to phosphoproteomics data interpretation, compared the use of tools with similar functions, and assessed the usability from the standpoint of typical biologists or clinicians. Overall, tools could be improved by standardization of enzyme names, flexibility of data input and output format, consistent maintenance, and detailed manuals.
What is the role of the practice nurse in the care of people living with dementia, or cognitive impairment, and their support person(s)?: a systematic review
Authors: Gibson Caroline , Goeman Dianne , Pond Dimity 
Published: July 2020

Abstract
Background
The potential value of expanding the Practice Nurse role to include the recognition and management of dementia has been acknowledged. Practice Nurses are well-positioned to provide comprehensive dementia information and support so that people living with dementia are better equipped to self-manage their health and live well with dementia. The purpose of this review was to systematically examine published literature to identify existing and potential roles of Practice Nurse’s in the delivery of care to people affected by dementia and to describe the characteristics and effectiveness of nurse interventions in dementia models of care.

Methods
The PRISMA statement guided the systematic review of the quantitative and qualitative evidence for roles and characteristics of the Practice Nurse in the delivery of dementia care. A comprehensive literature search of seven electronic databases and Google scholar identified relevant original research published in English between January 2000 and January 2019. Thirteen articles met the inclusion criteria and were extracted into the Covidence software for analysis.

Results
The heterogeneity of the included studies purpose, design and outcomes measures and the diversity in health systems and primary care nurses scope of practice made it difficult to synthesise the findings and draw conclusions. The heterogeneity did, however, provide important insights into the characteristics of roles undertaken by nurses working in the general practice setting, which were potentially beneficial to people living with dementia and their support person. These included patient accessibility to the Practice Nurse, early recognition and management of cognitive changes, care management and collaboration with the General Practitioner. Limitations of the provision of dementia care by Practice Nurses included a lack of definition of the role, inadequate dementia specific training, time constraints and poor communication with General Practitioners.

Conclusions
Embedding an evidence-based model that describes the role of the Practice Nurse in dementia care provision has the potential to increase early recognition of cognitive impairment and more appropriate primary care management of dementia.

Systematic review registration
PROSPERO 2018 CRD42018088191.
Increased circulating total bile acid levels were associated with organ failure in patients with acute pancreatitis
Authors: Xie Xiaochun , Dong Jie , Lu Guotao , Gao Kun , Li Xiaoyao , Mao Wenjian , Chen Faxi , Tong Zhihui , Li Baiqiang , Li Weiqin 
Published: July 2020

Abstract
Background
Recent studies have shown that bile acids (BAs) are closely related to metabolic and inflammatory diseases. Our study aimed to investigate whether circulating total bile acid (TBA) levels were associated with the severity of acute pancreatitis (AP).

Methods
We retrospectively collected data on patients diagnosed with AP in a tertiary center from 01 January 2014 to 31 December 2016. The highest TBA value during the first 1,2,3,5,7 days after admission was determined as D1, D2, D3, D5, D7 TBAmax. Patients were divided into the high TBA (HTBA) group and the normal TBA (NTBA) group according to whether the TBAmax was ≥10 μmol/L. The prognosis and complications, including death, organ failure (OF) and pancreatic necrosis, were compared between the two groups. Logistic regression analysis and receiving operating characteristic (ROC) curve were used to evaluate the relationship between circulating TBA and organ failure in AP patients.

Results
Through stratified analysis of each time period, we found that the incidence of OF in the HTBA group was significantly higher than that in the NTBA group, and the AP severity classification in the HTBA group was more serious than that in the NTBA group. In addition, according to the D7 TBAmax values, the pancreatic necrosis rate, percutaneous catheter drainage (PCD) rate and mortality in the HTBA group were higher than those in the NTBA group. Multivariate regression analysis showed that HTBA (odds ratio (OR), 4.894; P = 0.002) was an independent risk factor for AP complicated with OF, which was verified in the grouping based on D7 TBAmax. ROC analysis revealed that a circulating D7 TBAmax cutoff point of 6.450 umol/L had optimal predictive value for the development of OF in AP patients with an area under the curve of the ROC curve (AUCROC) of 0.777.

Conclusions
The increase of circulating TBA in early stage of AP is independently related to organ failure, which indicates the adverse prognosis of AP patients.
Rotavirus group A genotype circulation patterns across Kenya before and after nationwide vaccine introduction, 2010–2018
Authors: Mwanga Mike J. , Owor Betty E. , Ochieng John B. , Ngama Mwanajuma H. , Ogwel Billy , Onyango Clayton , Juma Jane , Njeru Regina , Gicheru Elijah , Otieno Grieven P. , Khagayi Sammy , Agoti Charles N. , Bigogo Godfrey M. , Omore Richard , Addo O. Yaw , Mapaseka Seheri , Tate Jacqueline E. , Parashar Umesh D. , Hunsperger Elizabeth , Verani Jennifer R. , Breiman Robert F. , Nokes D. James 
Published: July 2020

Abstract
Background
Kenya introduced the monovalent G1P [8] Rotarix® vaccine into the infant immunization schedule in July 2014. We examined trends in rotavirus group A (RVA) genotype distribution pre- (January 2010–June 2014) and post- (July 2014–December 2018) RVA vaccine introduction.

Methods
Stool samples were collected from children aged < 13 years from four surveillance sites across Kenya: Kilifi County Hospital, Tabitha Clinic Nairobi, Lwak Mission Hospital, and Siaya County Referral Hospital (children aged < 5 years only). Samples were screened for RVA using enzyme linked immunosorbent assay (ELISA) and VP7 and VP4 genes sequenced to infer genotypes.

Results
We genotyped 614 samples in pre-vaccine and 261 in post-vaccine introduction periods. During the pre-vaccine introduction period, the most frequent RVA genotypes were G1P [8] (45.8%), G8P [4] (15.8%), G9P [8] (13.2%), G2P [4] (7.0%) and G3P [6] (3.1%). In the post-vaccine introduction period, the most frequent genotypes were G1P [8] (52.1%), G2P [4] (20.7%) and G3P [8] (16.1%). Predominant genotypes varied by year and site in both pre and post-vaccine periods. Temporal genotype patterns showed an increase in prevalence of vaccine heterotypic genotypes, such as the commonly DS-1-like G2P [4] (7.0 to 20.7%, P < .001) and G3P [8] (1.3 to 16.1%, P < .001) genotypes in the post-vaccine introduction period. Additionally, we observed a decline in prevalence of genotypes G8P [4] (15.8 to 0.4%, P < .001) and G9P [8] (13.2 to 5.4%, P < .001) in the post-vaccine introduction period. Phylogenetic analysis of genotype G1P [8], revealed circulation of strains of lineages G1-I, G1-II and P [8]-1, P [8]-III and P [8]-IV. Considerable genetic diversity was observed between the pre and post-vaccine strains, evidenced by distinct clusters.

Conclusion
Genotype prevalence varied from before to after vaccine introduction. Such observations emphasize the need for long-term surveillance to monitor vaccine impact. These changes may represent natural secular variation or possible immuno-epidemiological changes arising from the introduction of the vaccine. Full genome sequencing could provide insights into post-vaccine evolutionary pressures and antigenic diversity.
Management of medically inoperable and tyrosine kinase inhibitor-naïve early-stage lung adenocarcinoma with epidermal growth factor receptor mutations: a retrospective multi-institutional analysis
Authors: Sun Yuemei , Wu Mengwan , Zhou Mingxiu , Luo Xing , Guo Yan , Bai Hansong , Zhang Zican , Tian Wei , Wang Xiaoshan , Bai Yifeng , Zhu Xueqiang , Pan Haixia , Deng Ying , Hu Honglin , Xia Jianling , Hao Xinbao , Han Liangfu , Wei Min , Liu Yingyi , Zeng Ming 
Published: July 2020

Abstract
Background
The clinical value of combined local radiation and epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) for medically inoperable and TKI-naïve early-stage lung adenocarcinoma patients with EGFR mutations has not yet been determined. In this study, we aimed to pool multi-institutional data to compare the therapeutic effect of EGFR-TKI treatment alone and combined radiation and TKI treatment on the survival outcomes in this patient subgroup.

Methods
A total of 132 cases of medically inoperable stage I to III EGFR mutant lung adenocarcinoma were retrospectively reviewed based on data from 5 centers. Among these patients, 65 received combined radiation and EGFR-TKI therapy (R + TKI) (49.2%), while 67 received EGFR-TKI (50.8%) treatment alone. All patients were followed until death.

Results
For the R + TKI group, the median overall survival (OS) after primary therapy was 42.6 months, while that of the TKI alone group was 29.4 months (log-rank p < 0.001). In terms of progression-free survival (PFS), the median PFS in these two treatment groups was 24 months and 14.7 months respectively (log-rank p < 0.001). Multivariate analysis showed that R + TKI was independently associated with improved OS (adjusted HR 0.420; 95% CI 0.287 to 0.614; p < 0.001) and PFS (adjusted HR 0.420; 95% CI 0.291 to 0.605; p < 0.001) compared to TKI alone. Subgroup analysis confirmed the significant OS benefits in stage III patients and RFS benefits in stage II/III patients.

Conclusions
Upfront radiation to primary sites with subsequent TKI treatment is a feasible option for patients with medically inoperable EGFR-mutant non-small-cell lung carcinoma (NSCLC) during first-line EGFR-TKI treatment, with significantly improved PFS and OS compared with those yielded by TKI treatment alone.