bluuebunny/crossref_metadata_2025_split huggingface dataset:
Technical Modifications in Parenchymal Transection During Laparoscopic Liver Resection Do Not Confer to an Increased Rate of Cut Surface Complications Compared to Open CUSA Based Liver Resections – A Single Center Comparative Analysis
Authors: Sucher Robert , Recknagel Sebastian , Guice Hanna , Sucher Elisabeth , Lederer Andri , Berg Thomas , Höhne Claudia , Seehofer Daniel 
Published: January 2021

Abstract
Background/AimsBiliary leakage (BL) is a major cause of postoperative morbidity after liver resection. Aim of our study was to analyse surgical parameters and postoperative morbidity with special emphasis on BL, after launching a minimally invasive liver resection program.MethodsA prospectively maintained medical database of patients who required a liver resection was used for analysis.ResultsA total of n=156 patients were divided into a group of n=47 patients (30.1%) receiving laparoscopic (LLR) and n=109 patients (69.9%) undergoing open liver resections (OLR). Patient age (OLR: 59.4 ± 16.0 vs. LLR: 57.9 ± 14.2 years) and male to female ratio (OLR: 63/46 vs. LLR: 25/22) were comparable. We performed n=75 (68.8%) major OLR and n=31 (66.0%) major LLR. Operation time was OLR 342.8 ± 110.5 min vs. LLR 287.3 ± 132.6 min (p=0.014) and the average blood loss was OLR 523.5 ± 428.6 ml vs. LLR 355.5 ± 459.2 ml. Morbidity and mortality was observed in n= 29 (18.6 %) and n= 7 (4.5 %) patients, respectively. The overall biliary leakage (BL) rate was 5.1% (n= 8). Majority of BL were detected in OLR with biliodigestive anastomosis (BDA) (n=2 (11.0%)) followed by OLR without BDA (n=6 (6.5%)). No BL were detected in patients with LLR. Hospital stay was significantly prolonged after OLR in patients with BL (38.4 ± 20.1 vs. 17.4 ± 11.1 days, p< 0.001).ConclusionIn line with previous reports, the introduction of different transection techniques in laparoscopic liver resections did not increase morbidity and BL-rate.
DnaJC7 binds natively folded structural elements in tau to inhibit amyloid formation
Authors: Joachimiak Lukasz , Hou Zhiqiang , Wydorski Pawel , Perez Valerie , Mendoza-Oliva Ayde , Ryder Bryan , Mirbaha Hilda , Kashmer Omar 
Published: January 2021

Abstract
Molecular chaperones, including Hsp70/Hsp40 families, play central roles in binding substrates to prevent their aggregation. How Hsp40s select different conformations of substrates remains poorly understood. Here, we report a novel interaction between the Hsp40 DnaJC7 and tau that efficiently suppresses tau aggregation in vitro and in cells. DnaJC7 binds preferentially to natively folded wild-type tau, but disease-associated mutants in tau reduce chaperone binding affinity. We identify that DnaJC7 uses a single TPR domain to recognize a β-turn element in tau that contains the 275VQIINK280 amyloid motif. Wild-type tau β-turn fragments, but not mutant fragments, can block full-length tau binding to DnaJC7. These data suggest DnaJC7 preferentially binds and stabilizes natively folded conformations of tau to prevent tau conversion into amyloids. This identifies a novel mechanism of tau aggregation regulation that can be exploited as both a diagnostic and a therapeutic intervention.
A Milroy Disease Family Caused by FLT4 Gene Mutation of c.2774 T&gt;A
Authors: Sui Yu , lu Yongping , Lin Meina , Ni Xiang , Chen Xinren , Li Huan , Jiang Miao 
Published: January 2021

Abstract
Background: Milroy disease (MD) is a rare, autosomal dominant disorder. Mutations in the Fms-related tyrosine kinase 4 (FLT4) gene cause the symptoms of this disease. In this report, we investigated the mutations in a large Chinese family with MD.Methods: We conducted Sanger sequencing of exons 17–26 of the FLT4 (NM_182925.4) gene. The primers were as follows: forward, 5' CTTCATCAGCGTCGAGTGG 3' and reverse, 5' ATTATGGGCGGGTTCCTT 3'. The amplification system is as follows: 2×Biotech Power PCR Mix, 10 µl; forward primer, 0.8 µl (10 µM); reverse primer, 0.8 µl (10 µM); DNA template, 1 µl (50 ng/µl); and ddH2O, 13.4 µl. The mutation was evaluated with MutationTaster, SIFT and PolyPhen.Results: A heterozygous substitution was detected in all patients but not in any healthy controls (FLT4 gene: c.2774 T>A, p.V925E). The mutation was predicted to be pathogenic.Conclusions: In this report, we described a large family with MD caused by a missense mutation of the FLT4 gene (c.2774 T>A, p.V925E).
Fire Suppression as Well as Seed Dispersal Play Critical Role in the Establishment of Forest Tree Species in Tropical Woodland
Authors: Fujita Tomohiro 
Published: January 2021

Abstract
This study examined the mechanisms of facilitation and importance of seed dispersal during establishment of forest tree species in a tropical woodland. Seedling survival of Syzygium guineense ssp. afromontanum seedling (forest tree species) was monitored for 2.5 years in four different microsites in savannah-woodland in Malawi under Ficus natalensis (a potential nurse plant), Brachystegia floribunda (a woodland tree), and Uapaca kirkiana (a woodland tree), and in a treeless site. S. guineense ssp. afromontanum seed deposition was also monitored in the four sites, and the natural establishment of forest tree species was observed to confirm the importance of seed dispersal. Protection from fire was found to be the most important facilitation mechanism in this area, with a total fire-induced mortality rate of 43%. However, the rate was comparatively low under F. natalensis, where fire is thought to be inhibited due to the lack of light-demanding C4 grasses. B. floribunda also offered protection from fire, and seedling survival did not differ between these two microsites. However, only a few individual forest tree species naturally established under B. floribunda, indicating that the facilitative mechanism of fire suppression is not the only factor affecting establishment in this tropical woodland. A higher rate of seed dispersal was also observed under F. natalensis compared with the other three microsites, suggesting that dispersal processes are also critical for the establishment of forest tree species in woodland in this region.
Effects of Exposure to Incense Smoke on Airway Function: An in Vitro and in Vivo Study
Authors: Yamamoto Norio , Kan-o Keiko , Tatsuta Miyoko , Ishii Yumiko , Tomohiro Ogawa , Shinozaki Seiji , Fukuyama Satoru , Nakanishi Yoichi , Matsumoto Koichiro 
Published: January 2021

Abstract
Recent clinical studies have suggested that inhalation of incense smoke (IS) may result in impaired lung function and asthma. However, there is little experimental evidence to link IS with airway dysfunction. Using mouse and cell culture models, we evaluated the effects of IS exposure on airway function, such as airway hyperresponsiveness (AHR), expression of multiple epithelial tight junction (TJ)- and adherens junction-associated mRNAs and proteins in the lungs, and the barrier function of bronchial epithelial cells assessed by transepithelial electronic resistance (TEER). Exposure of BALB/c mice to IS increased AHR and inflammatory macrophage recruitment to BALF; reduced claudin-1, -2, -3, -7, -10b, -12, -15, and -18, occludin, zonula occludens-1 [ZO-1], and E-cadherin mRNA expression; and caused discontinuity of claudin-2 and ZO-1 protein immunostaining in lung tissue. IS extract dose-dependently decreased TEER and increased reactive oxygen species production in bronchial epithelial cell cultures. Treatment with N-acetyl-l-cysteine, but not glucocorticosteroids or long-acting β2-agonists, prevented the detrimental effects of IS. IS exposure can be problematic for respiratory health, as evidenced by AHR, increased recruitment of inflammatory macrophages and disruption of TJ proteins in the lung, and damage to epithelial barrier integrity. However, antioxidants may be useful for the treatment of IS-induced airway dysfunction.
Effect of antibiotic treatment on Oxalobacter formigenes colonization of the gut microbiome and urinary oxalate excretion
Authors: Nazzal Lama , Francois Fritz , Henderson Nora , Liu Menghan , Li Huilin , Koh Hyunwook , Wang Chan , Gao Zhan , Perez Guillermo , Asplin John , Goldfarb David , Blaser Martin 
Published: January 2021

Abstract
Background: The incidence of kidney stones is increasing in the US population. Oxalate, a major factor for stone formation, is degraded by gut bacteria reducing its intestinal absorption. Intestinal O. formigenes colonization has been associated with a lower risk for recurrent kidney stones in humans. In the current study, we used a clinical trial of the eradication of Helicobacter pylori to assess the effects of an antibiotic course on O. formigenes colonization, urine electrolytes, and the composition of the intestinal microbiome. Methods: Of 69 healthy adult subjects recruited, 19 received antibiotics for H. pylori eradication, while 46 were followed as controls. Serial fecal samples were examined for O. formigenes presence and microbiota characteristics. Urine, collected serially fasting and following a standard meal, was tested for oxalate and electrolyte concentrations. Results: O. formigenes prevalence was 50%. Colonization was significantly and persistently suppressed in antibiotic-exposed subjects but remained stable in controls. Urinary pH increased after antibiotics, but urinary oxalate did not differ between the control and treatment groups. The O. formigenes-positive samples had higher alpha-diversity and significantly differed in Beta-diversity from the O. formigenes-negative samples. Specific taxa varied in abundance in relation to urinary oxalate levels.Conclusions: These studies identified significant antibiotic effects on O. formigenes colonization and urinary electrolytes and showed that overall microbiome structure differed in subjects according to O. formigenes presence. Identifying a consortium of bacterial taxa associated with urinary oxalate may provide clues for the primary prevention of kidney stones in healthy adults.
Age-dependent pathogenic characteristics of SARS-CoV-2 infection in ferrets
Authors: Kim Young-Il , Yu Kwang-Min , Koh June-Young , Kim Eun-Ha , Kim Se-Mi , Kim Eun Ji , Casel Mark Anthony , Rollon Rare , Jang Seung-Gyu , Song Min-Suk , Park Su-Jin , Jeong Hye Won , Kim Eung-Gook , Choi Younho , Lee Shin-Ae , Park Su-Hyung , Jung Jae U , Choi Young Ki 
Published: January 2021

Abstract
The sero-prevalence of SARS-CoV-2 in healthy people is not significantly different among age groups, but persons age 65 years or older had strikingly higher COVID-19 mortality compared to younger individuals. To understand COVID-19 manifestations in patients of different ages, ferrets in three age groups were infected with SARS-CoV-2. Although SARS-CoV-2 was isolated from all ferrets regardless of age, aged ferrets (≥3 years old) showed higher viral load, longer nasal virus shedding, and more severe lung inflammatory cell infiltration and clinical symptom compared to ≤6 months juvenile and 1-2 years young adult groups. Transcriptome analysis of aged ferret lungs revealed strong enrichment of gene sets related to type I interferon, activated T cell, and M1 macrophage responses, mimicking the gene expression profile of severe COVID-19 patients. Thus, SARS-CoV-2-infected aged ferrets highly recapitulate COVID-19 patients with severe symptoms and are useful for understanding an age-associated infection, transmission, and pathogenesis of SARS-CoV-2.
Adopt, adapt or abandon: Health care providers’ beliefs matter. Using the NASSS framework to analyse the implementation of four technology-supported person-centred care initiatives from the perspectives of health care providers
Authors: Dyb Kari , Berntsen Gro , Kvam Lisbeth 
Published: January 2021

Abstract
Background: Technology support and patient-centred care are the new mantra for health care programmes in Western societies. While few argue with the overarching philosophy of patient-centred care nor with the potential of information technologies, there is less agreement on how to make them a reality in everyday clinical practice. In this paper, we investigate how individual health care providers at four innovation arenas in Scandinavia experienced the implementation of technology-supported person-centred care for persons with long-term care needs by using the new analytical framework Nonadoption, Abandonment and challenges to the Scale-Up, Spread, and Sustainability of health and care technologies (NASSS). We also discuss the usability and sensitivity of the NASSS framework for those seeking to plan, implement and evaluate technology-supported health care programmes. This study is part of an interdisciplinary research and development project called Patients and Professionals in Partnership (2016 - 2020). It originates at one of nine work packages in this project.Method: The main data consist of ethnographic field observations at the four innovations arenas and 29 interviews with involved health care providers. To ensured continuous updates and status on work at the four innovation arenas, we have also participated in a total of six annual networks meeting arrange by the project. Results: While the NASSS framework is very useful indeed for identifying and communicating challenges with adoption and spread of technology-supported person-centred- care initiatives, we find it less sensitive towards capturing the dedication, enthusiasm and passion for care transformation that we found among the health care providers in our study. When it comes to technology-supported person-centred care, the point of no return has passed for the involved health care providers. To them, it is already a definite part of the future of healthcare services. How to overcome barriers and obstacles is pragmatically approached. Conclusion: Increased knowledge about the health care providers and their visions as potential assets for care transformation might be critical for those seeking to plan, implement and evaluate technology-supported health care programmes.
Continuous Deep Sedation Until Death Is Not an Alternative to Assisted Suicide in Palliative Care Units in the French-Speaking Part of Switzerland: A Multicentre Qualitative Study Based on Interviews With Physicians.
Authors: TOMCZYK Martyna , DIEUDONNÉ-RAHM Nathalie , JOX Ralf 
Published: January 2021

Abstract
Background: According to the European Association for Palliative Care, decisions regarding palliative sedation should not be made in response to a request for death. However, several studies show that continuous deep sedation until death (CDSUD) – a particular form of sedation – has been considered as an alternative to active assisted dying in some countries. In Switzerland, where assisted suicide is decriminalized and CDSUD is not legally regulated, no studies have comprehensively investigated if they might be correlated. Our study aimed to identify and describe the experience among palliative care physicians of CDSUD as an alternative to assisted suicide in the French-speaking part of Switzerland.Methods: We performed an exploratory multicentre qualitative study based on interviews with physicians working in palliative care institutions in the French-speaking part of Switzerland and conducted linguistic and thematic analysis of all interview transcripts. The study is described in accordance with COREQ criteria.Results: We included 10 interviews conducted in four palliative care institutions. Our linguistic analysis shows four main types of sedation, which we called ‘rapid CDSUD’, ‘gradual CDSUD’, ‘temporary sedation’ and ‘intermittent sedation’. CDSUD (rapid or gradual) is not considered an alternative to assisted suicide. In contrast, ‘temporary’ or ‘intermittent sedation’, although not medically indicated, is sometimes introduced in response to a request for assisted suicide. This is due to the impossibility of carrying out an assisted suicide at home (e.g., home transfer is impossible or the patient’s wish not to traumatize the family).Conclusion: These preliminary results can guide clinical, ethical, linguistic and legal reflection in this field and be used to explore this question more deeply at the national and international levels in a comparative, interdisciplinary and multiprofessional approach. They can also be useful to update Swiss clinical guidelines on palliative sedation in order to include specific frameworks on various sedation protocols and sedation as an alternative to assisted suicide. Potential negative impacts of considering palliative sedation as an alternative to assisted suicide should be nuanced by open and honest societal debate.
Effect of Parents’ Time With Children on the Relationship Between the Children’s Use of Electronic Devices and Their Sociability
Authors: Lee Ching-Fen , Tang Shain-May 
Published: January 2021

Abstract
Background: This study explored how parents’ roles of accompanying children influence the relationship between children’s use of electronic devices and their sociability. Methods: This study used data from Kids in Taiwan: National Longitudinal Study of Child Development and Care. Results: The results showed that parents’ time with their children on weekdays moderated the relationship between children using stationary devices and their sociability. However, time with children on weekends and holidays mediated the negative effect of children using mobile devices on their sociability. Conclusion: These findings have implications for future planners of childcare policies and educators regarding how to encourage parents to spend more time with their children and help them acquire sociability.
Is it Safe and Effective to Treat Complex Cervical Spondylotic Myelopathy Using Piezosurgery in Anterior Cervical Discectomy and Fusion?
Authors: Li Yuwei , Zhao Shixin , Wang Haijiao , Zhou Peng , Cui Wei , Xiao Wei , Li Fan , Hu Bingtao 
Published: January 2021

Abstract
Objective To investigate the safety and efficacy of piezosurgery in anterior cervical discectomy and fusion (ACDF) for treating patients with cervical spondylotic myelopathy (CSM) coincident retrovertebral body osteophytes adjacent to the endplate or a free nucleus pulposus migrated to the vertebral body, posteriorly; known as complex cervical spondylotic myelopathy (cCSM) here.Methods Forty-seven patients with cCSM underwent ACDF surgery from 2014 to 2017. Among these patients, 26 underwent ACDF using piezosurgery (group A) and 21 underwent ACDF by traditional tools such as air drill, bone curet and gun-shaped bone forceps (group B). Average operative duration, intraoperative blood loss, surgical complications, preoperative and postoperative Japanese Orthopaedic Association (JOA) scores and improvement rate were measured.Results Average operative duration and intraoperative blood loss were signiﬁcantly lower in the piezosurgery group than those in the traditional group (P < 0.01). The incidences of surgical complications were 3.8% and 23.8% in the piezosurgery and traditional groups (P < 0.05), respectively. Whereas JOA scores and improvement rate were insigniﬁcantly diﬀerent at each data collection period (P > 0.05); preoperative, postoperative 3 days and postoperative 1 year follow-up were included.Conclusion For treating cCSM, both the piezosurgery and traditional tools led to signiﬁcant neurological improvement. However, the piezosurgery was superior to the traditional tools in terms of operative duration, blood loss, and complication rate. Hence, the piezosurgery was a safe and effective adjunct for ACDF treating cCSM.
ANGPTL3 Gene Variants in Subjects With Familial Combined Hyperlipidemia
Authors: Bea Ana , Franco-Marin Esther , Marco-Benedí Victoria , Jarauta Estíbaliz , Gracia-Rubio Irene , Cenarro Ana , Civeira Fernando , Lamiquiz-Moneo Itziar 
Published: January 2021

Abstract
Angiopoietin-like 3 (ANGPTL3) plays an important role in lipid metabolism in humans. Loss-of-function variants in ANGPTL3 cause a monogenic disease named familial combined hypolipidemia. However, the potential contribution of ANGPTL3 gene in subjects with familial combined hyperlipidemia (FCHL) has not been studied. To study the gain-of-function (GOF) genetic variants in the ANGPTL3 gene in FCHL subjects and to establish the potential contribution of ANGPTL3 in the aetiology of FCHL. ANGPTL3 gene was sequenced in 162 unrelated subjects with severe FCHL and 165 normolipemic controls. Pathogenicity of genetic variants was predicted with PredictSNP2 and FruitFly. Frequency of identified variants in FCHL was compared with that of normolipemic controls and that described in the1000 Genomes Project. No GOF mutations in ANGPTL3 was present in subjects with FCHL. Four variants were identified in FCHL subjects, showing a frequency different from that observed in normolipemic controls: c.607-109T>C, c.607-47_607-46delGT, c.835+41C>A and c.*52_*60del. This last variant, c.*52_*60del, is a microRNA associated sequence in the 3’UTR of ANGPTL3, and it was present 2.7 times more frequently in normolipemic controls than in FCHL subjects. Our research shows that no GOF mutations in ANGPTL3 were found in a large group of unrelated subjects with FCHL.
Synchronization of Boron Application Methods and Rates is Environmental Friendly Approach to Improve Quality Attributes of Mangifera indica L. on Sustainable Basis
Authors: Khan Muhammad Mehboob Hassan , Ahmed Niaz , Ali Muhammad Arif , Danish Subhan , Skalicky Milan , Fahad Shah , Datta Rahul , Hassan Mohamed M. , Brestic Marian , Sabagh Ayman EL 
Published: January 2021

Abstract
Deficiency of micronutrients in soil is one of major cause for poor quality of mango fruit and yield. In addition, consumption of such diet also causes deficiency of micronutrients in humans. Among different micronutrients deficiency of boron is very common. It adversely affects flowering and pollen tube formation, thus played key role in decreasing the yield and quality attributes of mango. To overcome such problem, soil and foliar application of B is considered the efficacious methods. That’s why under changing climatic condition, a field experiment was conducted to explore the most suitable method and application rate of B in mango under current climatic scenario. There were nine treatments applied in three replications. Results showed that application of T8=RD+Borax (75 g plant-1 as basal application)+H3BO3 (0.8% as foliar spray) and T9=RD+Borax (150 g plant-1 as basal application)+H3BO3 (0.8% as foliar spray) significantly enhanced nitrogen, potassium, proteins, ash, fats, fiber and total soluble solids in mango as compared to control. A significant decrease in sodium, total phenolics contents, antioxidant activity and acidity as citric acid also validated the efficacious functioning of T8=RD+Borax (75 g plant-1 as basal application)+H3BO3 (0.8% as foliar spray) and T9=RD+Borax (150 g plant-1 as basal application)+H3BO3 (0.8% as foliar spray)over control. In conclusion T8=RD+Borax (75 g plant-1 as basal application)+H3BO3 (0.8% as foliar spray) and T9=RD+Borax (150 g plant-1 as basal application)+H3BO3 (0.8% as foliar spray)is an effective strategy to improve quality attributes of mango under changing climatic situation.