bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA MIR22HG promotes osteogenic differentiation of bone marrow mesenchymal stem cells via PTEN/ AKT pathway
Authors: Jin Chanyuan , Jia Lingfei , Tang Zhihui , Zheng Yunfei 
Published: July 2020

AbstractOsteoporosis is a prevalent metabolic bone disease characterized by low bone mineral density and degenerative disorders of bone tissues. Previous studies showed the abnormal osteogenic differentiation of endogenous bone marrow mesenchymal stem cells (BMSCs) contributes to the development of osteoporosis. However, the underlying mechanisms by which BMSCs undergo osteogenic differentiation remain largely unexplored. Recently, long non-coding RNAs have been discovered to play important roles in regulating BMSC osteogenesis. In this study, we first showed MIR22HG, which has been demonstrated to be involved in the progression of several cancer types, played an important role in regulating BMSC osteogenesis. We found the expression of MIR22HG was significantly decreased in mouse BMSCs from the osteoporotic mice and it was upregulated during the osteogenic differentiation of human BMSCs. Overexpression of MIR22HG in human BMSCs enhanced osteogenic differentiation, whereas MIR22HG knockdown inhibited osteogenic differentiation both in vitro and in vivo. Mechanistically, MIR22HG promoted osteogenic differentiation by downregulating phosphatase and tensin homolog (PTEN) and therefore activating AKT signaling. Moreover, we found MIR22HG overexpression promoted osteoclastogenesis of RAW264.7 cells, which indicated that MIR22HG played a significant role in bone metabolism and could be a therapeutic target for osteoporosis and other bone-related diseases.
How build angle and post-processing impact roughness and corrosion of additively manufactured 316L stainless steel
Authors: Melia Michael A. , Duran Jesse G. , Koepke Joshua R. , Saiz David J. , Jared Bradley H. , Schindelholz Eric J. 
Published: July 2020

AbstractAdditively manufactured austenitic stainless steels exhibit numerous microstructural and morphological differences compared to their wrought counterparts that will influence the metals corrosion resistance. The characteristic as-printed surface roughness of powder bed fusion (PBF) stainless steel parts is one of these morphological differences that increases the parts susceptibility to localized corrosion. This study experimentally determines the average surface roughness and breakdown potential (Eb) for PBF 316L in 6 surface finished states: as-printed, ground with SiC paper, tumble polished in abrasive media, electro-polished, chemically passivated, and the application of a contour/re-melt scan strategy. In general, a smaller average surface roughness led to a larger Eb. The smoothest surface treatments, ground and electro-polished conditions, led to Eb near the materials limit (~+1.0 VAg/AgCl) while all other surface treatments exhibited significantly lower Eb (~+0.3 VAg/AgCl) The build angle was also shown to impact surface roughness, where surfaces at high angles from the build direction resulted in larger roughness values, hence lower Eb.
Capnovolumetry in combination with clinical history for the diagnosis of asthma and COPD
Authors: Kellerer C. , Klütsch K. , Husemann K. , Sorichter S. , Jörres R. A. , Schneider A. 
Published: July 2020

AbstractCapnovolumetry performed during resting ventilation is an easily applicable diagnostic tool sensitive to airway obstruction. In the present analysis, we investigated in which way capnovolumetric parameters can be combined with basic anamnestic information to support the diagnosis of asthma and COPD. Among 1400 patients of a previous diagnostic study, we selected 1057 patients with a diagnosis of asthma (n = 433), COPD (n = 260), or without respiratory disease (n = 364). Besides performing capnovolumetry, patients answered questions on symptoms and smoking status. Logistic regression analysis, single decision trees (CHAID), and ensembles of trees (random forest) were used to identify diagnostic patterns of asthma and COPD. In the random forest approach, area/volume of phase 3, dyspnea upon strong exertion, s3/s2, and current smoking were identified as relevant parameters for COPD vs control. For asthma vs control, they were wheezing, volume of phase 2, current smoking, and dyspnea at strong exertion. For COPD vs asthma, s3/s2 was the primary criterion, followed by current smoking and smoking history. These parameters were also identified as relevant in single decision trees. Regarding the diagnosis of asthma vs control, COPD vs control, and COPD vs asthma, the area under the curve was 0.623, 0.875, and 0.880, respectively, in the random forest approach. Our results indicate that for the diagnosis of asthma and COPD capnovolumetry can be combined with basic anamnestic information in a simple, intuitive, and efficient manner. As capnovolumetry requires less cooperation from the patient than spirometry, this approach might be helpful for clinical practice.
A protective bivalent vaccine against Rift Valley fever and bluetongue
Authors: Calvo-Pinilla Eva , Marín-López Alejandro , Moreno Sandra , Lorenzo Gema , Utrilla-Trigo Sergio , Jiménez-Cabello Luis , Benavides Julio , Nogales Aitor , Blasco Rafael , Brun Alejandro , Ortego Javier 
Published: July 2020

AbstractRift Valley fever (RVF) and bluetongue (BT) are two important ruminant diseases transmitted by arthropods. Both viruses have shown important geographic spread leading to endemicity of BT virus (BTV) in Africa and Europe. In this work, we report a dual vaccine that simultaneously induces protective immune responses against BTV and RVFV based on modified vaccinia Ankara virus (MVA) expressing BTV proteins VP2, NS1, or a truncated form of NS1 (NS1-Nt), and RVFV Gn and Gc glycoproteins. IFNAR(−/−) mice immunized with two doses of MVA-GnGc-VP2 developed a significant neutralizing antibody response against BTV-4 and RVFV. Furthermore, the homologous prime-boost immunization with MVA-GnGc-NS1 or MVA-GnGc-NS1-Nt triggered neutralizing antibodies against RVFV and NS1-specific cytotoxic CD8+ T cells in mice. Moreover, all mice immunized with MVA-GnGc-NS1 or MVA-GnGc-NS1-Nt remained healthy after lethal challenge with RVFV or BTV-4. The homologous prime-boost vaccination with MVA-GnGc-NS1, which was the best immunization strategy observed in mice, was assayed in sheep. Clinical signs and viremia were absent or highly reduced in vaccinated sheep after challenge with BTV-4 or RVFV. These results indicate that MVA-GnGc-NS1 vaccination elicits immune protection against RVFV and BTV in sheep.
Disassembly-based bill of materials data for consumer electronic products
Authors: Babbitt Callie W. , Madaka Hema , Althaf Shahana , Kasulaitis Barbara , Ryen Erinn G. 
Published: July 2020

AbstractConsumer electronic products have a complex life cycle, characterized by environmental, social, and economic impacts and benefits associated with their manufacturing, use, and disposal at end-of-life. Accurately analysing these trade-offs and creating sustainable solutions requires data about the materials and components that make up these devices. Such information is rarely disclosed by manufacturers and only exists in the open literature in disparate case study format. This study presents a comprehensive database of bill of material (BOM) data describing the mass of major materials and components contained in 95 unique consumer electronic products. Data are generated by product disassembly and physical characterization and then validated against external benchmarks in the literature. The study also contributes a reproducible framework for organizing BOM data so that they can be expanded as new products enter the market. These data will benefit researchers studying all aspects of electronics and sustainability, including material scarcity, product design, environmental life cycle assessment, electronic waste policy, and environmental health and safety.
Presence of resveratrol in wild Arachis species adds new value to this overlooked genetic resource
Authors: Carvalho Paula Andreá Sampaio de Vasconcelos , de Carvalho Moretzsohn Márcio , Brasileiro Ana Cristina Miranda , Guimarães Patrícia Messenberg , da Silveira Agostini-Costa Tânia , da Silva Joseane Padilha , Gimenes Marcos A. 
Published: July 2020

AbstractGenusArachiscomprises 82 species distributed into nine taxonomic sections. MostArachisspecies are wild and those fromArachissection have been evaluated for many traits, since they can be used in peanut breeding. Most of the remaining species have been neglected and understudied. Recently, resveratrol content and expression of a resveratrol synthase gene were analyzed in wildArachisspecies. Our aim was to expand the knowledge about resveratrol inArachis, analyzing species from five sections and evaluating the expression of a resveratrol synthase (RS) gene responsive to ultraviolet light (UV) along the time. In a first experiment, the resveratrol content after UV induction was analyzed on detached leaves of 12 species from five sections. Variation was observed among species and accessions of the same species. The highest contents were found inA. lignosa(843.9 μg/g) andA. triseminata(745.4 μg/g).In a second experiment, RS expression and resveratrol content in four species and one synthetic amphidiploid were analyzed at 0, 7, 15 and 24 h pos induction (hpi) with UV. In most genotypes, the highest RS expression level was at 0 hpi, whereas the highest resveratrol content was at 15 hpi. Our results suggested that resveratrol is ubiquitously present in the genusArachiswith different capacities of synthesis among species and accessions in response to ultraviolet treatment. Presence of resveratrol in wildArachisspecies adds new value to these genetic resources.
Alteration of immunophenotype of human macrophages and monocytes after exposure to cigarette smoke
Authors: da Silva Camila Oliveira , Gicquel Thomas , Daniel Yoann , Bártholo Thiago , Vène Elise , Loyer Pascal , Pôrto Luís Cristóvão , Lagente Vincent , Victoni Tatiana 
Published: July 2020

AbstractCigarette smoke exposure (CS) is the main risk factor for chronic obstructive pulmonary disease (COPD). Macrophages have an important role in COPD because they release pro-inflammatory and anti-inflammatory cytokines. The present study’s we investigate the functional changes in macrophages and monocytes exposed to cigarette smoke extract (CSE). Herein, using human monocyte-derived macrophages (MDMs) from healthy donors and we found that CSE was not associated with significant changes in the production of pro inflammatory cytokines by MDMs. In contrast, exposure to CSE suppressed the production of IL-6 and Gro-a/CXCL1 by LPS-stimulated-MDMs, but had an additive effect on the release of IL-8/CXCL8 and MCP1/CCL2. However, CSE exposure was associated with greater production, TARC/CCL-17 and CCL22/MDC. Moreover, MDMs displayed a lower uptake capacity after CSE exposure. We identify, for what is to our knowledge the first time that monocytes from patients with COPD produced less IL-8/CXCL8 and Gro-α/CXCL1 after LPS stimulation and produced higher levels of TARC/CCL17 and MDC/CCL-22 after IL-4 stimulation. Our present results highlighted a skewed immune response, with an imbalance in M1 vs. M2 cytokine production. In conclusion, exposure to CS has contrasting, multifaceted effects on macrophages and monocytes. Our data may provide a better understanding of the mechanisms underlying COPD.
The circulating ANGPTL8 levels show differences among novel subgroups of adult patients with diabetes and are associated with mortality in the subsequent 5 years
Authors: Zou Huajie , Duan Wu , Zhang Zeqing , Chen Xi , Lu Puhan , Yu Xuefeng 
Published: July 2020

AbstractANGPTL8, an important regulator of glucose and lipid metabolism, is associated with diabetes, but the role of ANGPTL8 in the outcomes of novel subgroups of diabetes remains unclear. To assess the circulating ANGPTL8 levels in novel subgroups of diabetes and their association with health outcomes, we performed a data-driven cluster analysis (k-means) of patients with newly diagnosed diabetes (741 patients enrolled from 2011 through 2016) from the Risk Evaluation of Cancers in Chinese Diabetic Individuals: a longitudinal (REACTION) study. The primary outcomes were mortality from all causes and cardiovascular diseases (CVD), and the secondary outcome was any cardiovascular event. Comparisons among groups were performed using the Kruskal–Wallis test, and the correlations between variables were assessed using the Pearson correlation test. Logistic regression was used to detect associations between the risk of outcomes and the ANGPTL8 levels. We identified four replicable clusters of patients with diabetes that exhibited significantly different patient characteristics and risks of all-cause mortality. The serum ANGPTL8 levels in the cluster of mild age-related diabetes (MARD), severe insulin-resistant diabetes (SIRD), and severe insulin-deficient diabetes (SIDD) were significantly higher than those in the mild obesity-related diabetes (MOD) cluster (685.01 ± 24.50 vs. 533.5 ± 18.39, p < 0.001; 649.69 ± 55.83 vs. 533.5 ± 18.39, = 0.040; 643.29 ± 30.89 vs. 533.5 ± 18.39, p = 0.001). High circulating ANGPTL8 levels were more highly associated with a greater hazard of all-cause mortality (quartile 4 vs 1: risk ratio [RR] 3.23, 95% CI 1.13–9.22; per unit increase in the Z score: RR 1.53, 95% CI 1.17–2.01) than low circulating ANGPTL8 levels. In conclusion, this 5-year follow-up REACTION study revealed that the circulating ANGPTL8 levels show differences among novel subgroups of adult patients with diabetes and are associated with all-cause mortality in the subsequent 5 years.
Quality of life analysis after stereotactic radiofrequency ablation of liver tumors
Authors: Schullian Peter , Gertl Anja , Laimer Gregor , Putzer Daniel , Siebert Uwe , Levy Elliot , Bale Reto 
Published: July 2020

AbstractThe purpose of this study was to evaluate the health-related quality of life (HRQoL) after stereotactic radiofrequency ablation (SRFA) of liver tumors and identify variables associated with decreased HRQoL and to compare it to other treatments in case of concurrency. From 2011 to 2017 577 patients underwent SRFA for liver tumors in 892 ablation sessions. 303 (52.5%) patients completed a HRQoL questionnaire once after the ablation. HRQoL was assessed by the Short Form (SF)-12 health survey with mental and physical component scales and by a general questionnaire to assess disease and treatment-specific items as well as to compare tolerability of SRFA to transarterial chemoembolization (TACE), hepatic resection (HR) and chemotherapy (CTX). The median SF-12 PCS was 46.6 and MCS was 53.2. Patients experiencing pain or complications and patients refusing repeat SRFA showed significantly lower PCS (43.2 vs 48.6, p = 0.0003; 32.8 vs 46.9, p = 0.005 and 40.6 vs 46.9, p = 0.009). 355 (97.8%) patients indicated willingness to undergo repeat SRFA with little to no fear in 292 (80.7%) patients. Among patients with multiple therapies, SRFA was rated by 40 (90.9%) as preferred re-treatment, HR by 1 (2.3%) and CTX by 3 (6.8%). In conclusion, we have shown that SRFA has good HRQoL-outcomes and reported low morbidity rates. Consequently the vast majority of study patients would accept a repeated SRFA if necessary (97.8%), without fear (80.7%). SRFA was preferred among patients who experienced concurrent treatments, such as HR or CTX.
Survival of forensic trace evidence on improvised explosive devices: perspectives on individualisation
Authors: Vanderheyden Natasja , Verhoeven Elke , Vermeulen Steve , Bekaert Bram 
Published: July 2020

AbstractImprovised Explosive Devices (IEDs) are weapons of modern times, used by terrorist groups and thereby causing substantial damage to communities. There is a widespread misconception that destructive conditions like heat, water or pressure destroy all forensic evidence. Moreover, the plausibility to detect identifiable fingermarks and DNA on components of IEDs is insufficiently known. Therefore, this study investigated the effects of neutralisation and explosion on latent fingerprints and touch DNA. In a majority of the cases, comparative fingermark- and DNA testing resulted in individualisation. In some cases, despite extremely low amounts of contact DNA detected after deployment of render-safe tools or detonation, full STR profiles could be constituted, even after applying fingerprint development techniques. This research shows that latent fingerprints and touch DNA on improvised explosives can be successfully detected after destructive conditions and possibly be linked to the perpetrators of such crimes. This individualising power offers perspectives to enhance forensic investigations of terrorism-related crimes.
Modeling of entropy optimization for hybrid nanofluid MHD flow through a porous annulus involving variation of Bejan number
Authors: Shah Zahir , Sheikholeslami M. , Kumam Poom , Ikramullah  , Shafee Ahmad 
Published: July 2020

AbstractWe numerically investigate the non-Darcy magnetohydrodynamic hybrid nanoparticle migration through a permeable tank using control volume finite element method through entropy generation. The roles of various amounts of Permeability, Lorentz and Rayleigh (Ra) number are investigated upon the various aspects of the hybrid nanofluid flow through contour and 3-D plots. Through curve fitting technique, analytical expressions for Nuaveand Bejan number as functions of Ra, Ha and Da are obtained. It is found that the strength of the vortexes decline and temperature of the inner wall augments with the higher magnetic field, while temperature drops with increasing buoyancy forces and medium permeability. The irreversibility terms associated with the generation of the thermal energy and applied magnetic field (Sgen,th, Sgen,M) enhance while the other terms (Sgen,f, Sgen,p) drop with the rising values of the magnetic field strength. These quantities show exactly opposite behavior with augmenting Da. The Bejan number drops while Nuaveaugments with the rising buoyancy forces. The agreement with the previous published results confirms the accuracy of the employed computational model.
A century of precipitation trends in forest lands of the Lower Mississippi River Alluvial Valley
Authors: Ouyang Ying , Zhang Jiaen , Feng Gary , Wan Yongshan , Leininger Theodor D. 
Published: July 2020

AbstractVariations in long-term precipitation trends due to climate forcings have been observed in many parts of the world, exacerbating hydrological uncertainties to predicting droughts, floods, water resource availability, and ecosystem services. The Lower Mississippi River Alluvial Valley (LMRAV) is an important economic region of the midsouth USA, which is prone to natural disasters from extreme climate events and is known historically for cyclic flooding events and, within the last 20 years, for groundwater level declines. However, our knowledge of long-term precipitation trends in this region is fragmented. Using 100-year historic daily precipitation data from six stations of forest lands along with multivariate statistical analysis, we found that there were significant increasing trends (p ≤ 0.05) in annual precipitation near the south coastal area of the LMRAV and only marginally increasing trends in the northern area. Spatial variation in seasonality was observed at the decadal scale with increasing trends in fall near the coastal area and in spring around the north area. In addition to becoming wetter, the coastal area also experienced higher precipitation intensity with shorter return period over the past 100 years. These findings are useful to water resource managers for adapting to changing climate conditions in the LMRAV.
Graphitic carbon nitride/graphene nanoflakes hybrid system for electrochemical sensing of DNA bases in meat samples
Authors: Kalaiyarasi J. , Pandian K. , Ramanathan Santheraleka , Gopinath Subash C. B. 
Published: July 2020

AbstractThis research presents a simple, fast and simultaneous electrochemical quantitative determination of nucleobases, for example guanine (G), adenine (A), and thymine (T) in a beef and chicken livers samples to measure the quality of food products based on hybrids of graphitic carbon nitride/Graphene nanoflakes (g-C3N4/GNF) modified electrode. Graphitic carbon nitride (g-C3N4) made of graphite-like covalent link connects nitrogen, nitride, and carbon atoms in the structural design with improved the electrical properties and low band gap semiconductor. The g-C3N4/GNF nanocomposite was synthesized by the hydrothermal treatment to form a porous g-C3N4 interconnected three dimensional (3D) network of g-C3N4 and GNF. The 3D g-C3N4/GNF/GCE was utilized for the detection of nucleic acid bases with a well resolved oxidation peak for the individual analyte. The electrocatalytic current was established to be a linear range from 0.3 × 10–7 to 6.6 × 10–6, 0.3 × 10–7 to 7.3 × 10–6, and 5.3 × 10−6 to 63.3 × 10−4 M for G, A, and T with a detection limit of 4.7, 3.5 and 55 nM, respectively. The diffusion co-efficient and the kinetic parameters were derived from the chronoamperometry technique. The proposed sensing strategy has been effectively used for the application in real sample analysis and observed that the electrode free from the surface fouling.
Diurnal self-aggregation
Authors: Haerter Jan O. , Meyer Bettina , Nissen Silas Boye 
Published: July 2020

AbstractConvective self-aggregation is a modelling paradigm for convective rain cell organisation over a constant-temperature tropical sea surface. This set-up can give rise to cloud clusters developing over timescales of weeks. In reality, sea-surface temperatures do oscillate diurnally, affecting the atmospheric state and influencing rain rates significantly. Over land, surface temperatures vary more strongly. Here, we carry out a suite of cloud-resolving numerical experiments, and find that qualitatively different dynamics emerge from modest surface temperature oscillations: while the spatial distribution of rainfall is homogeneous during the first day, already on the second day, the rain field is firmly structured. In later days, this clustering becomes stronger and alternates from day to day. We show that these features are robust to changes in resolution, domain size and mean surface temperature, but can be removed by a reduction of the amplitude of diurnal surface temperature oscillation, suggesting a transition from a random to a clustered state. Maximal clustering occurs at a scale of$${l}_{\max }\approx 180\ {\rm{km}}$$lmax≈180km, which we relate to the emergence of mesoscale convective systems. At$${l}_{\max }$$lmax, rainfall is strongly enhanced and far exceeds the rainfall expected at random. Simple conceptual modelling helps interpret the transition to clustering, which is driven by the formation of mesoscale convective systems, and brings about day-to-day moisture oscillations. Our results may help clarify how continental extremes build up, and how cloud clustering over the tropical ocean could emerge as an instance of spontaneous symmetry breaking at timescales much faster than in conventional radiative–convective equilibrium self-aggregation.