bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dengue virus-activated platelets modulate monocyte immunometabolic response through lipid droplet biogenesis and cytokine signaling
Authors: Barbosa-Lima Giselle , Hottz Eugenio D , de Assis Edson F , Liechocki Sally , Souza Thiago Moreno L , Zimmerman Guy A , Bozza Fernando A , Bozza Patricia T 
Published: July 2020

Abstract
Dengue is characterized as one of the most important arthropod-borne human viral diseases, representing a public health problem. Increased activation of immune cells is involved in the progression of infection to severe forms. Recently, our group demonstrated the contribution of platelet–monocyte interaction to inflammatory responses in dengue, adding to evolving evidence that platelets have inflammatory functions and can regulate different aspects of innate immune responses. Furthermore, stimuli-specific-activated platelets can promote phenotypic changes and metabolic reprogramming in monocytes. Thus, this study aimed to evaluate the roles of dengue virus (DENV)-activated platelets on immunometabolic reprogramming of monocytes in vitro, focusing on lipid droplet (LD) biogenesis. We demonstrated that platelets exposed to DENV in vitro form aggregates with monocytes and signal to LD formation and CXCL8/IL-8, IL-10, CCL2, and PGE2 secretion. Pharmacologic inhibition of LD biogenesis prevents PGE2 secretion, but not CXCL8/IL-8 release, by platelet–monocyte complexes. In exploring the mechanisms involved, we demonstrated that LD formation in monocytes exposed to DENV-activated platelets is partially dependent on platelet-produced MIF. Additionally, LD formation is higher in monocytes, which have platelets adhered on their surface, suggesting that beyond paracrine signaling, platelet adhesion is an important event in platelet-mediated modulation of lipid metabolism in monocytes. Together, our results demonstrate that activated platelets aggregate with monocytes during DENV infection and signal to LD biogenesis and the secretion of inflammatory mediators, which may contribute to dengue immunopathogenesis.
Primary Central Nervous System Lymphoma: Clinical Evaluation of Automated Segmentation on Multiparametric MRI Using Deep Learning
Authors: Pennig Lenhard , Hoyer Ulrike Cornelia Isabel , Goertz Lukas , Shahzad Rahil , Persigehl Thorsten , Thiele Frank , Perkuhn Michael , Ruge Maximilian I. , Kabbasch Christoph , Borggrefe Jan , Caldeira Liliana , Laukamp Kai Roman 
Published: July 2020

BackgroundPrecise volumetric assessment of brain tumors is relevant for treatment planning and monitoring. However, manual segmentations are time‐consuming and impeded by intra‐ and interrater variabilities.PurposeTo investigate the performance of a deep‐learning model (DLM) to automatically detect and segment primary central nervous system lymphoma (PCNSL) on clinical MRI.Study TypeRetrospective.PopulationSixty‐nine scans (at initial and/or follow‐up imaging) from 43 patients with PCNSL referred for clinical MRI tumor assessment.Field Strength/SequenceT1−/T2‐weighted, T1‐weighted contrast‐enhanced (T1 CE), and FLAIR at 1.0, 1.5, and 3.0T from different vendors and study centers.AssessmentFully automated voxelwise segmentation of tumor components was performed using a 3D convolutional neural network (DeepMedic) trained on gliomas (n = 220). DLM segmentations were compared to manual segmentations performed in a 3D voxelwise manner by two readers (radiologist and neurosurgeon; consensus reading) from T1 CE and FLAIR, which served as the reference standard.Statistical TestsDice similarity coefficient (DSC) for comparison of spatial overlap with the reference standard, Pearson's correlation coefficient (r) to assess the relationship between volumetric measurements of segmentations, and Wilcoxon rank‐sum test for comparison of DSCs obtained in initial and follow‐up imaging.ResultsThe DLM detected 66 of 69 PCNSL, representing a sensitivity of 95.7%. Compared to the reference standard, DLM achieved good spatial overlap for total tumor volume (TTV, union of tumor volume in T1 CE and FLAIR; average size 77.16 ± 62.4 cm3, median DSC: 0.76) and tumor core (contrast enhancing tumor in T1 CE; average size: 11.67 ± 13.88 cm3, median DSC: 0.73). High volumetric correlation between automated and manual segmentations was observed (TTV: r = 0.88, P < 0.0001; core: r = 0.86, P < 0.0001). Performance of automated segmentations was comparable between pretreatment and follow‐up scans without significant differences (TTV: P = 0.242, core: P = 0.177).Data ConclusionIn clinical MRI scans, a DLM initially trained on gliomas provides segmentation of PCNSL comparable to manual segmentation, despite its complex and multifaceted appearance. Segmentation performance was high in both initial and follow‐up scans, suggesting its potential for application in longitudinal tumor imaging.Level of Evidence3Technical Efficacy Stage2
Influence of Nature of Spacer and Hydrocarbon Chain Length on Micellar Encapsulation of Polynuclear Aromatic Hydrocarbons by Carbohydrate Derived Non‐Ionic Gemini Surfactants in Aqueous Ethanol Medium
Authors: Saroj  , Sharma Lalit 
Published: July 2020

AbstractGemini surfactants with rigid aromatic spacer ‐CH2‐Ar‐CH2‐: 6,6′‐(N,N′‐di(alkyl)‐1,4‐phenylenedimethylamino)bis(6‐deoxy‐1,2‐O‐isopropylidene‐α‐D‐glucofuranose) and Gemini surfactants with flexible aliphatic spacer ‐(CH2)6‐: 6,6′‐(N,N′‐di(2‐hydroxyalkyl)‐1,6‐hexanediamino)bis(6‐deoxy‐1,2‐O‐isopropylidene‐α‐D‐glucofuranose) have been synthesized, and their micellar properties for encapsulation of polynuclear aromatic hydrocarbons (PAH) viz. fluorene, anthracene, triptycene, and pyrene in aqueous ethanol medium have been studied by means of electronic spectroscopy. The micellar study reveals that nature of spacer and length of hydrocarbon chain of the surfactant has profound effect on micellar encapsulation of PAH. The surfactants with rigid aromatic spacer show greater encapsulation as compared to surfactants with flexible aliphatic spacer. Moreover, the more hydrophobic surfactant, i.e. the surfactant with a longer hydrocarbon chain, shows greater encapsulation toward PAH which are encapsulated in the order of their smaller size.
Novel Structural Increments for Estimating the Krafft Temperature of Ionic Surfactants in Aqueous Solutions
Authors: Zieliński Ryszard 
Published: July 2020

AbstractIn this work, we report results of our investigation on the relationship between the Krafft temperature of ionic surfactants in aqueous solutions and their chemical structure. We tested empirical values of the Krafft temperature available in literature for aqueous solutions of 234 ionic surfactants and correlated them to the surfactant chemical structure. In this work, we propose a novel set of 67 group contributions for calculation of the Krafft temperature of ionic surfactants in aqueous solutions. Using them, the Krafft temperature values in aqueous solutions for 174 anionic, 43 cationic, and 17 zwitterionic surfactants were calculated and the estimated values obtained in this way were compared with experimental values. We have demonstrated that the newly developed set of numerical values structural group contributions of the surfactants allows correct estimation of the Krafft temperature value for the tested set of data with the maximum error 7.6°C and the mean square error 3.7°C for various types of ionic surfactants in aqueous solutions.
Postharvest temperature and water status influence postharvest splitting susceptibility in summer radish (Raphanus sativus L.)
Authors: Lockley Rachel A , Beacham Andrew M , Grove Ivan G , Monaghan James M 
Published: July 2020

AbstractBACKGROUNDSplitting is a problem that seriously affects appearance and marketability in a number of fruit and vegetables. In summer radish (Raphanus sativus L.), splitting can occur during growth, harvesting and postharvest. We investigated the factors affecting splitting susceptibility in summer radish cv. Celesta during postharvest handling.RESULTSSplitting susceptibility was negatively related to temperature, with higher temperature reducing splitting due to dropping impact. Radish diameter was positively associated with compression failure force, suggesting that larger radishes are more resistant to compressive splitting. An increase in radish hypocotyl water content (WC) was associated with an increase in splitting susceptibility due to impact and decrease in failure force for both compression and puncture forces. Increased hypocotyl WC may increase splitting susceptibility by increasing the water potential of the radish tissue. In agreement, we found that increased hypocotyl WC was associated with higher internal water potential in radish tissue.CONCLUSIONSWe therefore recommend that the hypocotyl WC of summer radish crops be managed during the harvest and postharvest phases, and that crops are processed at higher, ambient, temperature in order to reduce splitting, before storing at low temperature and high humidity to maintain quality and shelf life. © 2020 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Open‐tubular capillary electrochromatographic application of a sol‐gel matrix with chilli peppers, garlic, or synthetic additives
Authors: Svobodová Jana , Mikšík Ivan 
Published: July 2020

AbstractThis article describes a possible combination of two promising fields of analytical chemistry—the preparation of sol‐gel matrices with varying additives and their application in capillary electrochromatography. The inner surfaces of capillaries were coated with the sol‐gel solution containing either pure synthetic chemical additive—alliin or capsaicin—or an extract of their natural sources—garlic and chilli pepper, respectively. The modified capillaries were tested for interaction with two neurotransmitters, oligopeptides and nucleotides under conditions of open‐tubular capillary electrochromatography. Because both of the natural extracts also contain vitamin C and saccharose, the capillaries with sol‐gel modifiers containing each of these substances were also tested. The obtained results from the perspective of changes in the electrochromatograms and the effective mobilities of analytes are discussed with respect to mild conditions both in the preparation process of the sol‐gel matrix and during the separations.
Simultaneous quantification of three tropane alkaloids in goji berries by cleanup of the graphene/hexagonal boron nitride hybrids and ultra‐high‐performance liquid chromatography tandem mass spectrometry
Authors: Zhao Weihua , Shi Yanping 
Published: July 2020

AbstractA modified quick, easy, cheap, effective, rugged and safe method was established for simultaneous determination of atropine, anisodamine, and scopolamine in goji berries by using ultra‐high‐performance liquid chromatography with tandem mass spectrometry. The graphene/hexagonal boron nitride hybrids were prepared and first applied as a cleanup adsorbent. Compared to classical cleanup adsorbent (C18), the graphene/hexagonal boron nitride hybrids as adsorbent had better extraction efficiency for the detection of analytes. Under the optimal conditions, the proposed analytical method achieved satisfactory linearity (R2 > 0.995), and obtained desirable recoveries ranged from 77.4 to 94.0% with the relative standard deviation of 1.2–6.1% at the concentration levels of 3.2–13.4 µg/kg. The limits of quantitation of atropine, anisodamine, and scopolamine were, respectively, 3.2, 4.6, and 4.5 µg/kg with linearity ranged from 3.2 to 25.4 µg/kg. The modified quick, easy, cheap, effective, rugged, and safe sample preparation with ultra‐high‐performance liquid chromatography and tandem mass spectrometry method was successfully applied to evaluate the safety of goji berries collected from 30 plant areas in China, suggesting its applicability and suitability for the routine analysis of three tropane alkaloids in goji berries.
Moral Injury and Social Well‐Being: A Growth Curve Analysis
Authors: Chesnut Ryan P. , Richardson Cameron B. , Morgan Nicole R. , Bleser Julia A. , Perkins Daniel F. , Vogt Dawne , Copeland Laurel A. , Finley Erin 
Published: July 2020

AbstractMoral injury (MI) may occur in the context of committing transgressions (i.e., self‐directed MI reactions), witnessing transgressions, or being the victims of others’ transgressions (i.e., other‐directed MI reactions) that violate an individual's moral principles. Veterans with MI may experience impaired social well‐being (SWB). Studies on MI and veterans’ SWB have focused almost exclusively on social support and used cross‐sectional data. The present study used growth curve analyses to examine the associations between self‐ and other‐directed MI reactions and veterans’ levels of social support, social functioning, social activities, and social satisfaction over the first 18 to 21 months of their transition to civilian life (N = 9,566). The results demonstrated declines in all SWB outcomes, with self‐ and other‐directed MI reactions having differential effects. Higher versus lower levels of other‐directed MI reactions were related to lower baseline scores on all SWB outcomes, βs = −.06 to −.20, and steeper declines over time in social functioning, β = −.09, and social satisfaction, β = −.10. Higher versus lower levels of self‐directed MI reactions were related to lower baseline levels of social functioning, β = −.07, but higher baseline levels of social activity, β = .04. Higher versus lower levels of self‐directed MI reactions were related to a steeper decline in social activity over time, β = −.10. These findings present a more nuanced picture than that depicted by current MI theoretical frameworks and support further research to uncover moderators of the associations between self‐ and other‐directed MI reactions and SWB outcomes.
All‐Printed Electronic Skin Based on Deformable and Ionic Mechanotransducer Array
Authors: Kim Joo Sung , Choi Hanbin , Hwang Hee Jae , Choi Dukhyun , Kim Do Hwan 
Published: July 2020

AbstractMechanoreceptors in human skin possess high sensitivity, wide sensing range, and high sensing resolution for external stimuli. Several attempts have been made to implement electronic skin (e‐skin) that can mimic human skin. However, previous attempts are limited by the fundamental resolution problem arising from the use of film‐like materials generated through pouring and spinning processes. Here, an all‐printed e‐skin based on deformable ionic mechanotransducer array (IMA) inspired by the physiological tactile sensing mechanism and the geometric features of mechanoreceptors in human skin is described. First, an ionic mechanotransduction channel is emulated with a piezocapacitive ionic mechanosensory system that engages in ion migration when the polymer matrix is deformed under a mechanical non‐equilibrium state. Furthermore, the versatile shapes of the artificial mechanotransducer are tuned by the printing process variables, which results in high sensitivity (2.65 nF kPa−1) and high resolution (13.22 cm−2) of the device. It is demonstrated that this IMA is fully bio‐inspired by the mechanotransduction and papillary structure of the mechanoreceptors. A high‐resolution e‐skin with a deformable and transparent IMA, which is fabricated by an all‐printing methodology, will open up a new market in the field of soft and stretchable sensory platforms.
Antihypoxic Action of Panax Japonicus, Tribulus Terrestris and Dioscorea Deltoidea Cell Cultures: In Silico and Animal Studies
Authors: Lagunin Alexey , Povydysh Maria , Ivkin Dmitry , Luzhanin Vladimir , Krasnova Marina , Okovityi Sergei , Nosov Alexander , Titova Maria , Tomilova Svetlana , Filimonov Dmitry , Poroikov Vladimir 
Published: July 2020

AbstractChemical diversity of secondary metabolites provides a considerable variety of pharmacological actions with a significant extension due to their combinations in plant extracts. Production of plant‐derived medicinal products in cell cultures has advantages because of the efficient use of different biotic and abiotic elicitors and better control of the developmental processes. Using PASS software, we predicted biological activity spectra for phytoconstituents identified in cell cultures of Panax japonicus (12 molecules), Tribulus terrestris (4 molecules), and Dioscorea deltoidea (3 molecules). Mechanisms of action associated with the antihypoxic effect were predicted for the majority of molecules. PharmaExpert software allowed analyzing possible synergistic or additive effects of the combinations of phytoconstituents associated with the antihypoxic action. Experimental studies of the antihypoxic effect of the plants′ extracts in water and ethanol have been performed in 3 animal models: Acute asphyctic hypoxia (AAH), Acute haemic hypoxia (AHeH), and Acute histotoxic hypoxia (AHtH). Effects of Panax japonicus and Tribulus terrestris preparations exceeded the activity of the reference drug Mexidol in the AHtH model. In the AHeH model, all preparations demonstrated moderate activity; the most potent has been observed for Dioscorea deltoidea. Thus, we found that experimental studies in animal models have confirmed the in silico prediction.
miRNA/Target Gene Profile of Endothelial Cells Treated with Human Triglyceride‐Rich Lipoproteins Obtained after a High‐Fat Meal with Extra‐Virgin Olive Oil or Sunflower Oil
Authors: Santiago‐Fernandez Concepción , Martin‐Reyes Flores , Bautista Rocío , Tome Mónica , Gómez‐Maldonado Josefa , Gutierrez‐Repiso Carolina , Tinahones Francisco J. , Garcia‐Fuentes Eduardo , Garrido‐Sánchez Lourdes 
Published: July 2020

ScopeThe effects of triglyceride‐rich lipoproteins (TRLs) on the miRNA expression of endothelial cells, which are very involved in atherosclerosis, according to the type of diet are not known.Methods and ResultsThe differences between the effects of TRLs isolated from blood of subjects after a high‐fat meal with extra‐virgin olive oil (EVOO) and sunflower oil (SO) on the microRNA‐Seq profile related to atherosclerosis in human umbilical vein endothelial cells are analyzed. 28 upregulated microRNAs with EVOO‐derived TRLs, which can regulate 22 genes related to atherosclerosis, are found. 21 upregulated microRNAs with SO‐derived TRLs, which can regulate 20 genes related to atherosclerosis, are found. These microRNAs are mainly involved in angiogenesis, with a predominance of an anti‐angiogenic effect with EVOO‐derived TRLs. Other microRNAs upregulated with SO‐derived TRLs are involved in cardiovascular diseases. Pathways for the target genes obtained from the upregulated microRNA with EVOO‐derived TRLs are involved in lipid metabolism and inflammatory and defense response, while those with SO‐derived TRLs are involved in lipid metabolic process.ConclusionEVOO‐derived TRLs seem to produce a more atheroprotective profile than SO‐derived TRLs. This study provides alternative mechanisms on the protective role of EVOO against the atherogenic process through microRNA regulation in endothelial cells.
Highly accurate GaN HEMT model based on the Angelov model with error compensation
Authors: Zhao Ziyue , Lu Yang , Zhang Hengshuang , Yi Chupeng , Wang Yuchen , Ma Xiaohua , Hao Yue 
Published: July 2020

AbstractWith the development of the microelectronics, circuit design has become more and more important. In order to improve the accuracy of circuit design, it is necessary to improve the accuracy of the circuit model. This letter proposes an accurate method based on the Angelov model of the Gallium Nitride (GaN) high electron mobility transistors (HEMTs). For Angelov model, the fitting of DC curve is critically important. There are many parameters to fit and modify the fitting curve. Each parameter in the Angelov model has a physical meaning. Some parameters have significant influence on the fitting accuracy of the model. In this letter, the parameters sensitive to the accuracy of the model are evaluated, and formulated into a function of gate voltage and drain voltage. In order to further improve the accuracy of the model, the IV curves are compensated using the error function. A weight function is introduced to correct the error function and improve its compensation selectivity. The proposed method is an universal and effective method that significantly improves the accuracy of the Angelov model. The introduction of the error function and the weight function improve the accuracy obviously compared with the traditional Angelov model.
Shape detection of multiple subsurface cavities by particle filtering with elastic wave propagation
Authors: Takamatsu Ryosuke , Fujisawa Kazunori , Nakahata Kazuyuki , Murakami Akira 
Published: July 2020

SummaryA numerical technique for detecting the number and shape of subsurface cavities is presented, applying the particle filter and the parametric level set method to elastic wave propagation under the ground. When subsurface cavities exist, the elastic wave propagating in the ground is reflected at the boundary faces of the cavities. Observing the velocity of the reflection wave at the surface of a ground that includes multiple cavities and parameterizing the shape of the cavities by the parametric level function, both the number and the shape of the cavities can be identified by the particle filter. Numerical experimentation for detecting multiple cavities is conducted with synthetic observation data. The results show that the proposed technique enables the number of cavities to be identified by the number of peaks in the posterior probabilistic distribution function and solves geometric inverse problems by estimating the shape of the cavities through the parameter identification of the level set function.
Should we routinely assess psychological morbidities in idiopathic lower urinary tract dysfunction: ICI‐RS 2019?
Authors: Tarcan Tufan , Selai Caroline , Herve François , Vrijens Desiree , Smith Phillip P. , Apostolidis Apostolos , Panicker Jalesh N. , Kirschner‐Hermanns Ruth , Arlandis Salvador , Mosiello Giovanni , Dmochowski Roger , Cardozo Linda , von Gontard Alexander 
Published: July 2020

AbstractAimsPsychological morbidities play a major role in idiopathic lower urinary tract dysfunction (iLUTD). The aim of the Think Tank (TT) was to discuss the relevance of psychological morbidities in idiopathic LUTD over the life span, including overactive bladder (OAB) or dysfunctional voiding (DV) and methods of assessment.MethodsThe paper is based on a selective review of the literature and in‐depth discussions, leading to research recommendations regarding the assessment of psychological morbidities in iLUTD on children and adults held during the TT of the International Consultation on Incontinence Research Society in 2019.ResultsPsychological comorbidities affect the health behaviors and treatment outcomes in patients with iLUTD. Both clinically relevant comorbid mental disorders, as well as subclinical psychological symptoms have a major impact and negatively influence incontinence treatment. Research is needed to elucidate mechanisms underlying iLUTD and psychological comorbidities. Clinical studies are needed to determine how perception generation and cognition impacts on the relationship of urinary perceptions, symptoms, and objective urodynamic function. Due to high psychological comorbidity rates, screening with validated, generic questionnaires for emotional and behavioral disorders in children with nocturnal enuresis, daytime urinary incontinence, and fecal incontinence is recommended. Brief screening is recommended for all adults with iLUTD, especially with OAB and DV, who are refractory to treatment.ConclusionsDue to the high rate and relevance in clinical practice, screening for psychological comorbidities is recommended for all age groups. The research recommendations of this TT may be followed to improve the assessment of psychological morbidities in iLUTD.