bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vitamin value assessment of fruits and berries in the Central Black Earth Region (CBER) by the level of biologically active substances in the wild analogues
Authors: Morgacheva N V , Zakharov V L , Petrisheva T Yu , Sotnikova E B 
Published: September 2020

Abstract
The fruits of all wild plants were gathered in Tambov, Lipetsk and Voronezh regions. The following research methods were used: thermogravimetric, titrimetric, photometric. Fruit weight of cultivars is 2.8 (the cherry) – 54.2 (the strawberry) times bigger than the fetal weight of wild related species. The rowan, the apple, the pear, the plum, the hawthorn, the current, the blackberry, the raspberry and the apricot are in the range. The selection of fruit and berries of the Central Black Earth Region provided: the reduction of organic acid by a factor of 1.8-6.9 in the fruits of the apple, the pear, the hawthorn, the briar, the cherry, the blackberry, the raspberry, the guilder rose, the apricot; the rise of ascorbic acid by a factor of 1.5-10 in the fruits of the rowan, the apple, the briar, the blackberry, the raspberry, the guilder rose, the apricot; the rise of β – carotene by a factor of 1.5-10 in the fruits of the apples, the hawthorn, the briar, the cherry, the current and the blackberry; the rise of anthocyans by the factor of 2-26.8 in the fruits of the rowan, the apple, the pear, the hawthorn, the briar, the black current, the blackberry, the guilder rose, the strawberry and the apricot; the rise of flavonols by the factor of 1.5-13.7 in the fruits of the apple, the rowan, the pear, the plum, the briar; the rise of catechins by a factor of 2.4-827 in the fruits of the apple, the rowan, the pear, the plum, the hawthorn, the briar, the current, the blackberries, the raspberry, the guilder rose, the apricot; the percentage rise of solids by a factor of 1.5 in the fruits of the cherry and the apricot; the rise of tannins and colorants by the factor of 1.5-3.2 in the fruits of the rowan, the hawthorn and the apricot; the rise of the tanninum by the factor of 3.1-3.5 in the fruits of the rowan and the hawthorn as well as increasing in the amount of carotenoids by the factor of 1.5 in the fruits of the raspberry. The rise of solids, carotinoids, tannins and colorants including tanninum content in the fruit and berries turned out to be the most complicated for research results of fruit and berry selection.
Assessment of winter triticale genotypes in the selection of grain-feed varieties for the foothill zone of the Central Caucasus
Authors: Manukyan I R , Miroshnikova E S 
Published: September 2020

Abstract
The article presents the results of evaluation of winter triticale initial material for economically valuable traits for grain and forage areas breeding varieties. The object of research were winter triticale cultivars of different ecological and geographical origin: Hortenso, Moderato (Poland), Prader (Switzerland), TGI 22/1, GR-16/2 (Russia), 88 T 142 KS (USA). Grade Valentin 90The was taken as standard. Statistical methods for evaluating grades plasticity and stability as well as plant productivity index (PPI) were used to assess the genotypes adaptability. It is specified that yields above 1 t/ha on average over the three years showed the following cultivars: Moderato (1,05 t/ha), GR 16/2 (1,14 t/ha) and Hortenso (1.19 t/ha). The following cultivars: Valentin 90 (13,2), Hortenso (12,7), GR 16/2 (13,5), Moderato (11,6) were highly productive according to PPI. In terms of ecological plasticity varieties were divided into groups: the value of bi close to one – varieties well adapted to the conditions of the region (Prader, Hortenso, GR 16/2), extensive varieties – bi less than unity (KS 88 T 142), intensive varieties – bi is greater than unity (Valentin 90, TGI 22/1, Moderato). In terms of homeostasis and breeding values the cultivars were distributed in the following order: Hortenso, GR-16/2, Valentin 90, Prader, TGI 22/1, Moderato, KS 88 T 142. Additional data obtained by the provocative sloping sites to determine the potential productivity and aftergrowth capacity. The best regeneration ability showed the following cultivars: Hortenso, GR 16/2, Moderato, Prader.
Technology of the application of microbial preparations on ordinary chernozem soil in the zone of unstable moistening of the Central Ciscaucasia
Authors: Faizova V I , Tskhovrebov S V , Lysenko V Y , Novikov A A 
Published: September 2020

Abstract
Studies were conducted in the zone of unstable moistening of the Central Ciscaucasia in the ordinary chernozem carbonate according to the scheme: 1. control 2. KBP-standard; 3. KBP – innovative; 4. PCM; 5. KMP-92. According to the traditional technology was carried out treatment of seeds before sowing and maize plants in the phase of 3-4 leaves and flowering, and on the proposed produce and processing of the soil. Revealed that the number of aerobic nitrogen-fixing bacteria on control in phase 3-4 sheet amounted to 21.1 thousand CFU/1 g, and in the flowering stage 76.3 thousand CFU/1 g and increased with the application of KBP-book standard respectively 2.2 and 1.7 times, KBP – innovative 1.9 and 1.6 times, PCM 2.1 and 1.4 times and the PCM-92 – 1.7 and 1.4 times. The lowest values have on the phase of wax ripeness. The number of ammonifying control in the phase of 3-4 leaves was 55 million CFU/1 g. and has increased as a result of the application of biopreparations in 1,4-1,6 times. In the flowering stage, the number of ammonifying control increased to 259 million CFU/1 g and increased by different versions from 1.6 to 1.7 times. Similar changes can be traced in the number of nitrifying microorganisms. The yield of corn for grain was lowest on control (of 4.07 t/ha), increased more than conventional technology, the embodiment with the use of KBP-S 24.1 %, and the proposed technology with the use of KMP-92 - 31.4%.
Organization of keeping animals in the aviary
Authors: Moiseeva T A 
Published: September 2020

Abstract
The article is devoted to the rules of keeping animals in aviaries. The conditions of keeping animals in zoos and the organization of their care are considered. It is said that the premises for the maintenance of wild animals should provide their biological, species and individual needs, the cubic capacity of the premises should ensure the freedom of movement of animals, the presence of sufficient air and light. The minimum norms of the area of the premises for keeping wild animals and cells sizes for the cellular keeping of animals are given, factors affecting the optimal microclimate in the rooms for animals are listed. The methods of maintaining cleanliness in aviaries, methods of caring for the skin and hair of animals, as well as the features of bathing animals are examined in detail. It is stated that a prerequisite for keeping animals in captivity is the presence of a veterinary service or a doctor, their responsibilities are listed, the rules for calculating the standard number of staff of veterinary specialists for the annual volume of veterinary work in zoo are given.
Antibiotic resistance of microorganisms isolated from a Baikal seal
Authors: Pliska A A , Ryadinskaya N I , Batomunkuev A S , Ponomarev D A 
Published: September 2020

Abstract
The article presents the results of microbiological studies of the material taken from the seals’ corpses. The authors studied the taxonomic characteristics of microorganisms, their virulence and antibiotic resistance. It has been established that the following types of microorganisms are found in the seal: Citrobacter braakii, S. warneri, S. aureus, Escherichia coli serotypes O2, O18 and O39, Lactobacillus sakei, Aguamicrobium lusatiense. All pathogens isolated from the gastrointestinal tract were sensitive to 52.6% of antibiotics (ampicillin, cefuroxime, cefixime, gentamicin, amikacin, ciprofloxacin, ofloxacin, furadonin, Ceftriaxone) and resistant to 15.8% of antibiotics, two representatives of cephalosporins (cefaclor and ceftazidim) and ticarcillin. Conditionally pathogenic enterobacteria (Citrobacter braakii and Escherichia coli) isolated from muscles and liver were sensitive to all antibiotics.
Species composition and distribution of blood-sucking Diptera insects of the Tyumen region
Authors: Khlyzova T A , Fiodorova O A 
Published: September 2020

AbstractAs a result of the analysis of literature data and materials from many years of our research, 108 species and 1 subspecies of blood-sucking dipterous insects were found to live in the Tyumen region. The horsefly fauna consists of 35 species and 1 subspecies belonging to 6 genera, blood-sucking mosquitoes are represented by 40 species of 5 genera, blood-sucking midges - 16 species of 10 genera, blood-sucking biting midges - 17 species of the 1st genus. The greatest species diversity is characteristic of the subzone of small-leaved aspen-birch forests, where 95 species and 1 subspecies are found. To date, the fauna of dipterans subzones of the middle taiga remains poorly studied. The group of dominants in the southern taiga is represented by horsefliesH. ciureai, H. bimaculata, H. muehlfeldiandH. pluvialis, mosquitoesA. cantans, midgesB. maculatusandS. pusilla, wood liceC. punctatus. In the subzone of small-leaved aspen-birch forests,horseflies T. bromius, A. fulvus, A. rusticus, H. ciureai, H. bimaculata, H. pluvialisandH. subcylindrica, mosquitoesA. cinereusandA. cantans, midgesB. maculatusandS. pusilla, woodlicesC. punctatus. The dominant species in the forest-steppe zone are horsefliesT. bromius, T. bovinus,H. ciureai, H. bimaculata, H. pluvialisandH. subcylindrica, mosquitoesA. punctorandA. cantans, midgesB. maculatus, S. pusillaandB. erythrocephala, woodlicesC. punctatus.
Growth and development of Kochia prostrate (L.) Schrad in the extraordinary year in the north desert of Atyrau region in Kazakhstan
Authors: Mukhambetov B , Abdinov R , Alzhanova R , Kadasheva Zh 
Published: September 2020

Abstract
A system of agriculture is being developed to accumulate and preserve moisture in soil strips (5-10 cm wide, 5 cm deep, repeated every 50-55 cm in virgin soil untouched by vegetation), treated by surface raking of the loose seedbed layer to form a solid compacted bed, where an ideal condition for seed germination is created. With a favorable combination of weather and climatic conditions, this variant provides an optimal fullness of seedlings and a fairly high fruit-bearing weight of 3.9 t/ha on average for four years, including 4,8 tons in 2019. However, in extraordinary 2019-2020 crop year with extremely low precipitation (2,7 times lower than average annual norms), with the warm winter and spring (100 more than the average annual norm), incredibly intense droughts and dust storms (average of 18-19 days a month vs 4-5 mean annual norm) wind erosion dominated and dictated terms everywhere: furrows, sown with seeds of Summer Cypress were completely covered with drifts of dust with a thickness of 2-3 cm, under which the seedlings could not break through to the surface of the soil and died. In the cultivar keeping nursery, where seeds were sown directly without tillage and 15 PCs/m2 of seedlings were obtained only in the dust-inspired areas that make up one third of the area (400 m2).
Adaptive anti-oxidative responses to chronic exposure to stress-signaling molecule, oxidized cell-free DNA, in rat neural cells
Authors: Filev A D , Kostyuk S V , Pisarev V M , Tabakov V Yu , Umriukhin P E 
Published: September 2020

AbstractStress is an adaptation reaction to harmful environment changes. Both oxidized and non-oxidized cell-free DNA (oxo-cfDNA and no-cfDNA) liberating from damaged cells possesses a characteristic of stress-molecules. CfDNA molecules modified by oxidation exhibit more oxidative (increasing 8-oxodG in DNA) and antioxidative (enhanced transcription ofNRF2and NRF2-dependent genes) activities than no-cfDNA in non-neural cell cultures. Whether the oxo-cfDNA molecules affect brain cells under different regimens of treatment that may mimic multiple, chronically applied stressful insults remained unclear. We studied if multiple stimulations of nervous system cells with oxidized cfDNA may further augment the DNA oxidative damage by inducing 8-oxodG modifications, and increase antioxidantHmox1gene expression and the NRF2 protein level in a primary cell culture of rat cerebellum (RCC). We found that daily treatment of RCC with oxo-cfDNA for 3 days increased accumulation of 8-oxodG in intracellular DNA, activated transcription ofHmox1gene and enhanced the NRF2 protein content. However, after eleven-day daily treatment, the opposite effect was observed, in which 8-oxodG level in intracellular DNA andHmox1gene expression and NRF2 protein level in cerebellar cells were significantly decreased. Therefore, under subchronic, 3-day regimen of treatment, oxidized cfDNA further augmented accumulation of 8-oxodG in cellular DNA and promoted activation of the antioxidant system whereas its 11-day chronic action decreased both activities presumably due to inducing tolerance to stress-signaling via overactivation and/or exhaustion of molecular targets of oxo-cfDNA in cerebellar cells.
Study of the influence of external factors on the inheritance of forms of dissociants Pseudomonas mandelii
Authors: Vafina G Kh , Stupak E E 
Published: September 2020

Abstract
In the course of studying the phase variations of the Pseudomonas mandellii strain IB-Ki14, the pH of the medium was found to be the main factor inducing the switching of dissociants on LB medium. Dissociants of the P. mandellii strain IB-Ki14 are stably inherited at pH 5.0-6.0. Dissociant cells differed in antioxidant and protease-producing abilities. In general, matte colony cells had higher antioxidant and proteolytic activity. At the same time, the cells of the mucous colonies had a higher extracellular Arg-X protease activity compared to the matte ones and, accordingly, were able to hydrolyze a larger amount of protein from the nutrient medium. An analysis of the Arg-X protease activity of the dissociant cell fractions revealed differences at the level of 0.35 M NaCl fraction (loosely bound to the cell residue), which is quite possibly associated with the localization of proteolysis in a specific subcellular region, which allows spatial control of the content of regulatory proteins.
The influence of green-manured and complete fallow on soil fertility and wheat productivity in Tuva
Authors: Zhulanova V N , Ulyanova O A , Zharova T F , Sambuu A D 
Published: September 2020

Abstract
The influence of complete, seeded, and green manured fallows on the chemical and agrophysical properties of dark chestnut soil in the conditions of the forest steppe of Tuva is studied. It was determined that the humus content in green manured fallows increased by 0.13%–0.64% during rotation. It was revealed that various types of fallows do not have a strong effect on the density of composition and structural composition of dark chestnut soil. The highest biological activity of soils is observed in crop rotations with green manured and complete manured fallows with organic fertilizers. It was determined that complete manured fallow with manure and green manured fallows increase spring wheat yield.
Novel approach to protein-protein interaction assessment
Authors: Gilmiyarova F N , Kolotyeva N A , Kuzmicheva V I , Remizov V V , Gusyakova O A 
Published: September 2020

Abstract
Past decades, the study of proteins and small molecules interaction has been at least lagging behind the study of other types of interactions such as protein-protein, protein-DNA and protein-RNA. The aim of this study is to evaluate the effect of low-molecular biologically active compounds, lactate and pyruvate, on protein-protein interactions and to provide quantitative assessment. Using AB0 blood groups system model and labeled monoclonal anti-A and anti-B antibodies we studied the effect of natural metabolites lactate and pyruvate on antigen-antibody interaction. Erythrocytes of A(II) and B(III) blood groups were incubated with lactate and pyruvate before the agglutination reaction. Then the agglutination reaction was performed. Visualization of agglutination complexes was carried out by means of confocal laser scanning microscopy with further processing of noise pixels. A series of in vitro experiments with further visualization and mathematical processing showed the co-directed but more pronounced effect of lactate on the antigen-antibody reaction as compared to pyruvate. In case of influence on antigen A the aggregation process intensification was noted, while this process was inhibited in case of influence on antigen B. The obtained results demonstrate possibility of using small molecules, in particular lactate and pyruvate, as molecular probes and prospects of erythrocytes with antigenic determinants of AB0 system expressed on their membranes for studying protein-protein interactions due to visualization clarity and possibility of quantitative assessment of this process. It is proved that lactate and pyruvate can act as regulators of protein-protein interactions on the example of antigen-antibody reaction.
Productivity and feed value of sparsely distributed annual crops
Authors: Avetisyan A T , Baykalova L P , Artemyev O S , Martynova O V 
Published: September 2020

Abstract
The feed value of sparsely distributed annual crops in the forest-steppe of the Krasnoyarsk territory was determined, namely: the content of feed units, feed protein units, digestible protein; the yield of green mass, the collection of dry matter, feed protein units (FPU), feed units (FU) and energy productivity. The feed value and productivity of annual grasses depended on the biological characteristics of the species and variety, the content of nutrients, the yield of green mass and the collection of dry matter. There were increases in the green mass of Echinochloa frumentacea (Roxb.) Link, Amaranthus paniculatus and Melilotus albus to control Avena sativa L. by 182 %, 148% and 145 %. The best in collecting dry matter, feed protein units, digestible protein and energy productivity were Melilotus albus and Echinochloa frumentacea (Roxb.) Link. Panicumm iliaceum L has the maximum content of feed units in the green mass 0.23 feed units/kg, Melilotus albus has 0.25 FPU/kg of feed protein units and Panicum miliaceum L. has 0.24 FPU/kg, Melilotus albus has 35 g/kg of digestible protein.
Incidence and seasonal variation of pet dermatophytosis in Moscow region
Authors: Savinov V A , Kapustin A V , Ovchinnikov R S , Shastin P N , Laishevtsev A I 
Published: September 2020

Abstract
In the current one-year study 2477 samples from pets clinically suspected for dermatophytosis (1010 from dogs and 1467 from cats) were examined by direct microscopy, conventional mycological culture and DTM culture. Direct microscopy revealed 1,28% positive samples, conventional culture – 8,0% and DTM culture – 27,7% positive samples. The average incidence of dermatophytosis in Moscow region was 14% (9,5% in cats and 4,5% in dogs), most of cases were diagnosed in autumn. DTM culture proved to be the most effective method for diagnosing dermatophytosis in pets.
Bioethics as effective method for preserving biological diversity on Earth
Authors: Barmashova T I , Lazutkina E V 
Published: September 2020

Abstract
Man is not the only living creature on our planet. In order to remain man in the highest sense of the word, he must build his relations on the basis of the principle of humanism, not only with his own kind, but also with all living beings. Bioethics is essentially a kind of a model of a person’s relationship with all living things in this world. However, while the worldview principle of anthropocentrism dominates the world, the principles of bioethics cannot be fully realised. Such an attitude not only violates the principle of co-evolution, but inevitably destroys the spiritual world of man. The aim of this article is to explicate bioethics as humanism which gives the relationship between humans and animals an axiological character in which the life of an animal is considered of value. The work emphasises the relevance of replacing the principle of anthropocentrism with the principle of biocentrism, which implies a humane attitude to everything living in the world. The principles of bioethics are more consistent with the principle of harmonious coexistence of man and nature, the implementation of the proper attitude of man to living beings, located below him on the evolutionary ladder. The process of personality education should involve not only the acquisition of knowledge, the assimilation of moral norms of behaviour regarding people, but also the formation of their practical readiness and the need for a humane attitude to animals. An important task of mankind is conservation of biological diversity on our planet.
Morphofunctional changes in the organs of excretion and detoxification with pesticides prolonged low-dose exposure
Authors: Gerunova L K , Bardina E G , Sechkina I V 
Published: September 2020

Abstract
Many toxic substances in low doses daily enter the body of animals and humans with food. In this case, the main burden of the transformation and excretion from the body have the organs of excretion and detoxification. This review summarizes the results obtained on different experimental models and reflecting the impact of pesticides on the gastrointestinal tract, liver and kidneys at low doses chronic intoxication. It is established that, entering the body in low doses, pesticides cause a change in the morphological structure of the intestine and the enzymatic activity of the enterocytes, as well as help reduce the barrier function of the gastrointestinal tract and the development of dysbiosis. When exposed to pesticides there are also changes of biochemical processes in the liver and decrease its functional activity, impaired kidney structure. The original state of health and genetic features of stress-limiting systems determine the severity of the changes and level of post-toxic complications risk.
Assessment of the ecological state of the Boguchany reservoir by zoobenthos organisms
Authors: Andrianova A V 
Published: September 2020

AbstractZoobenthos communities had been studied in the middle and lower parts of the Boguchany reservoir for five years (2014-2015, 2017-2019). The benthic fauna was chironomid-gammarid in nature with a predominance of chironomids. The littoral communities are qualitatively and quantitatively much richer than in the profundal. With a decrease in depth, there was an increase in the number of invertebrates by about 6 times due to the small-sized species of chironomids. The trophic status of the Boguchany reservoir in 2018 corresponded to the mesotrophic type of medium food capacity (III class), in other years - to the oligotrophic type of low food capacity (II class). Currently, the formation of bottom fauna is at the stage of biocenosis stabilization. An integrated approach was used to assess water quality according to bottom communities. The fundamental criterion for the selection of indices was the consideration of the benthocenosis structural organization, i.e. the development degree of indicator taxa. Water quality, assessed by the state of zoobenthos communities, corresponded to III class, “Moderately polluted”, β-mesosaprobic zone.