bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emodin regulates cell cycle of non-small lung cancer (NSCLC) cells through hyaluronan synthase 2 (HA2)-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathway
Authors: Li Mingzhu , Jin Shengbo , Cao Yang , Xu Jian , Zhu Shendong , Li Zheng 
Published: January 2021

Abstract
Background
Non-small cell lung cancers (NSCLC) account for most cases of lung cancer. More effort is needed to research new drug and combination therapies for this disease. An anthraquinone derivative, emodin shows anticancer potency. We hypothesis that emodin suppresses lung cancer cells through hyaluronan (HA) synthase 2-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathway mediated cell cycle regulation.

Methods
We tested the effect of emodin on viability, apoptosis, and HA secretion of 5 NSCLC cell lines. We used NSCLC cells A549 for two rounds of knockdown study: (1) knocking down either the synthases (HAS2 and HAS3) or the receptors (CD44 and RHAMM); (2) knocking down either HAS2 or HAS3. Then determined the effect of emodin on viability, HA secretion, cell cycle, and expression of cyclin proteins.

Results
Emodin suppressed viability and HA secretion of all 5 NSCLC cell lines except for HA secretion of H460. Emodin had a slight apoptosis induction effect on all cell lines and was not different among cell lines. The knockdown of either the synthases or the receptors blocked emodin effects on viability while the knockdown of HAS2 block emodin effects but not HAS3. Emodin increased cells in the G1/G0 phase, and decreased cells in the S and G2/M phase by down-regulating cyclin A and B and up-regulating cyclin C, D, and E. HAS2 knockdown blocked the effects of emodin on the cell cycle.

Conclusions
This study demonstrated that emodin regulates the cell cycle of NSCLC cells through the HAS2-HA-CD44/RHAMM interaction-dependent signaling pathway.
Single blinded semi-field evaluation of MAÏA® topical repellent ointment compared to unformulated 20% DEET against Anopheles gambiae, Anopheles arabiensis and Aedes aegypti in Tanzania
Authors: Mbuba Emmanuel , Odufuwa Olukayode G. , Tenywa Frank C. , Philipo Rose , Tambwe Mgeni M. , Swai Johnson K. , Moore Jason D. , Moore Sarah J. 
Published: January 2021

Abstract
Background
N,N-Diethyl-3-methylbenzamide (DEET) topical mosquito repellents are effective personal protection tools. However, DEET-based repellents tend to have low consumer acceptability because they are cosmetically unappealing. More attractive formulations are needed to encourage regular user compliance. This study evaluated the protective efficacy and protection duration of a new topical repellent ointment containing 15% DEET, MAÏA® compared to 20% DEET in ethanol using malaria and dengue mosquito vectors in Bagamoyo Tanzania.

Methods
Fully balanced 3 × 3 Latin square design studies were conducted in large semi-field chambers using laboratory strains of Anopheles gambiae sensu stricto, Anopheles arabiensis and Aedes aegypti. Human volunteers applied either MAÏA® ointment, 20% DEET or ethanol to their lower limbs 6 h before the start of tests. Approximately 100 mosquitoes per strain per replicate were released inside each chamber, with 25 mosquitoes released at regular intervals during the collection period to maintain adequate biting pressure throughout the test. Volunteers recaptured mosquitoes landing on their lower limbs for 6 h over a period of 6 to 12-h post-application of repellents. Data analysis was conducted using mixed-effects logistic regression.

Results
The protective efficacy of MAÏA® and 20% DEET was not statistically different for each of the mosquito strains: 95.9% vs. 97.4% against An. gambiae (OR = 1.53 [95% CI 0.93–2.51] p = 0.091); 96.8% vs 97.2% against An. arabiensis (OR = 1.08 [95% CI 0.66–1.77] p = 0.757); 93.1% vs 94.6% against Ae. aegypti (OR = 0.76 [95% CI 0.20–2.80] p = 0.675). Average complete protection time (CPT) in minutes of MAÏA® and that of DEET was similar for each of the mosquito strains: 571.6 min (95% CI 558.3–584.8) vs 575.0 min (95% CI 562.1–587.9) against An. gambiae; 585.6 min (95% CI 571.4–599.8) vs 580.9 min (95% CI 571.1–590.7) against An. arabiensis; 444.1 min (95% CI 401.8–486.5) vs 436.9 min (95% CI 405.2–468.5) against Ae. aegypti.

Conclusions
MAÏA® repellent ointment provides complete protection for 9 h against both An. gambiae and An. arabiensis, and 7 h against Ae. aegypti similar to 20% DEET (in ethanol). MAÏA® repellent ointment can be recommended as a tool for prevention against outdoor biting mosquitoes in tropical locations where the majority of the people spend an ample time outdoor before going to bed.
Geographic variation and determinants of help seeking behaviour among married women subjected to intimate partner violence: evidence from national population survey
Authors: Muluneh Muluken Dessalegn , Alemu Yeshemebet Worku , Meazaw Maereg Wagnew 
Published: January 2021

Abstract
Background
Help seeking behaviour amongst married women who experienced Intimate Partner Violence (IPV) has received limited attention in Africa. This study examines the geographic variation and investigates determinants of help seeking behaviour amongst married women in Ethiopia.

Methods
This study analysed data from the 2016 Ethiopian Demographic and Health Survey (EDHS). Data was extracted for married women age 15–49 years old who experienced IPV. Factors associated with help seeking behaviour were identified using multiple logistic regression adjusted for clustering and weighing. The weighted proportion of factors associated with help seeking behaviour was exported to ArcGIS to conduct autocorrelation analysis.

Results
The prevalence of help seeking behaviour among married women who experienced IPV was 19.8% (95% CI: 15.9–24.3%). Only 9.2% of them sought help from a formal source (such as police, lawyer or doctor). Multiple logistic regression analyses showed physical violence (Adjusted odds ratio (AOR)=2.76), educational attainment (AOR=2.1), a partner’s alcohol consumption (AOR=1.9), partner’s controlling behaviour (AOR= 2.4), partner’s employment status, (AOR= 1.9) and wealth index (AOR=2.8) were significantly associated factors with help seeking behaviour among married women who experienced IPV in Ethiopia (P< 0.05). Women in Benishangul-Gumuz, Gambella, Harari, Western and Eastern Amhara, and Afar had the lowest odds of help seeking behaviour (P< 0.001) after experiencing IPV.

Conclusion
The findings of this study suggest that poor help seeking behaviour for married women experiencing IPV is a significant public health problem in Ethiopia. Multiple interrelated factors were associated with poor help seeking behaviour. These factors include women’s level of educational attainment, women experiencing physical violence, partners exhibiting controlling behaviour, partner’s alcohol consumption, the employment status of the partner, and wealth status of the household were important predictors of help seeking behaviour. Policies and interventions need to be tailored to address these factors to improve women’s health outcomes and to prevent IPV.
Comparison of viromes in vaginal secretion from pregnant women with and without vaginitis
Authors: Zhang He-Teng , Wang Hao , Wu Hai-Sheng , Zeng Jian , Yang Yan 
Published: January 2021

Abstract
Background
Although some studies have investigated the bacterial community in vaginal tract of pregnant women, there are few reports about the viral community (virome) in this type of microenvironment.

Methods
To investigate the composition of virome in vaginal secretion samples, 40 vaginal secretion samples from pregnant women with vaginitis and 20 vaginal secretion samples from pregnant women without vaginitis, pooled into 4 and 2 sample pools, respectively, were subjected to viral metagenomic analysis.

Results
Results indicated virus sequences showing similarity to human papillomavirus (HPV), anellovirus, and norovirus were recovered from this cohort of pregnant women. Further analysis indicated that 15 different defined types and one unclassified type of HPV were detected from pregnant women with vaginitis while only 3 defined types of HPV were detected in pregnant women without vaginitis. Five different groups of viruses from the family Anelloviridae were present in pregnant women with but none of them were detected in pregnant women without vaginitis. Norovirus was detected in 3 out of the 4 sample pools from pregnant women with vaginitis but none in the pregnant women without vaginitis. Twelve complete genomes belonging to 10 different types of HPV, and 5 novel anllovirus genomes belonging 2 different genera in Anelloviridae were acquired from these libraries, based on which phylogenetical analysis and pairwise sequence comparison were performed. Phageome in these samples was also briefly characterized and compared between two groups.

Conclusion
Our data suggested that virome might play an important role in the progression of vaginitis in pregnant women.
Management of late onset urea cycle disorders—a remaining challenge for the intensivist?
Authors: Redant S. , Empain A. , Mugisha A. , Kamgang P. , Attou R. , Honoré P. M. , De Bels D. 
Published: January 2021

AbstractBackgroundHyperammonemia caused by a disorder of the urea cycle is a rare cause of metabolic encephalopathy that may be underdiagnosed by the adult intensivists because of its rarity. Urea cycle disorders are autosomal recessive diseases except for ornithine transcarbamylase deficiency (OTCD) that is X-linked. Optimal treatment is crucial to improve prognosis.Main bodyWe systematically reviewed cases reported in the literature on hyperammonemia in adulthood. We used the US National Library of Medicine Pubmed search engine since 2009. The two main causes are ornithine transcarbamylase deficiency followed by type II citrullinemia. Diagnosis by the intensivist remains very challenging therefore delaying treatment and putting patients at risk of fatal cerebral edema. Treatment consists in adapted nutrition, scavenging agents and dialysis. As adults are more susceptible to hyperammonemia, emergent hemodialysis is mandatory before referral to a reference center if ammonia levels are above 200 µmol/l as the risk of cerebral edema is then above 55%. Definitive therapy in urea cycle abnormalities is liver transplantation.ConclusionAwareness of urea cycle disorders in adults intensive care units can optimize early management and accordingly dramatically improve prognosis. By preventing hyperammonemia to induce brain edema and herniation leading to death.
Refinements of some integral inequalities for unified integral operators
Authors: Jung Chahn Yong , Farid Ghulam , Andrić Maja , Pečarić Josip , Chu Yu-Ming 
Published: January 2021

AbstractIn this paper we are presenting the refinements of integral inequalities established for convex functions. Consequently, we get refinements of several fractional integral inequalities for different kinds of fractional integral operators.
Body mass index and blood urea nitrogen to creatinine ratio predicts refeeding hypophosphatemia of anorexia nervosa patients with severe malnutrition
Authors: Funayama Michitaka , Mimura Yu , Takata Taketo , Koreki Akihiro , Ogino Satoyuki , Kurose Shin 
Published: January 2021

Abstract
Aim
To investigate development of refeeding hypophosphatemia during the refeeding period and the extent of the decrease in the serum phosphorus level among anorexia nervosa patients with severe malnutrition.

Objective
The accurate prediction of the severity of refeeding hypophosphatemia in patients with anorexia nervosa during acute treatment is of great importance. Although some predictors were found in previous reports, these studies used binominal data—the presence or absence of hypophosphatemia—as an outcome indicator but not the extent of serum phosphorus level decrease. It is crucial in clinical settings to predict the extent of the serum phosphorus level decrease as well as development of refeeding hypophosphatemia, in particular, for patients with severe malnutrition, who has a higher risk of death.

Methods
We investigated 63 admissions from 37 patients with anorexia nervosa who had severe malnutrition (admission body mass index 11.5 ± 1.6) and carried out a linear discriminant regression analysis for the development of refeeding hypophosphatemia. The extent of the decrease in the serum phosphorus level were investigated using multiple linear regression analysis. Explanatory variables included data upon admission (age, sex, body mass index, blood urea nitrogen to creatinine ratio, albumin, initial serum phosphorus level, anorexia nervosa type, i.e., restrictive or binge-purge) as well as treatment-related indicators (calorie intake, amount of phosphate administered, and rate of weight gain).

Results
Development of refeeding hypophosphatemia and a change in serum phosphorus levels were predicted by body mass index and elevated blood urea nitrogen to creatinine ratio.

Conclusions
Our study found that refeeding hypophosphatemia among patients with severe malnutrition was predicted by a lower body mass index and elevated blood urea nitrogen to creatinine ratio.
Designing a Permissioned Blockchain Network for the Halal Industry using Hyperledger Fabric with multiple channels and the raft consensus mechanism
Authors: Surjandari Isti , Yusuf Harman , Laoh Enrico , Maulida Rayi 
Published: January 2021

AbstractHalal Supply Chain Management requires an assurance that the entire process of procurement, distribution, handling, and processing materials, spare parts, livestock, work-in-process, or finished inventory to be well documented and performed fit to the Halal and Toyyib. Blockchain technology is one alternative solution that can improve Halal Supply Chain as it can integrate technology for information exchange during the tracking and tracing process in operating and monitoring performance. This technology could improve trust, transparency, and information disclosure between supply chain participants since it could act as a distributed ledger and entitle all transactions to be completely open, yet confidential, immutable, and secured. This study uses a Blockchain Network with three channels and uses raft consensus algorithm in designing web interfaces and testing their capabilities. From the web interface, there were no failures in the validity test during the invoke test and the query test. In addition, the web interface was also successfully tested to thwart the formation of a block in case of data input errors from the user. The server can also do the process as a provider of information and validator for the web interface. From the results of simulations conducted on the Blockchain Network that was made, Blockchain’s transaction speed is fast and all the transaction is successfully transferred to other peers. Thus, Permissioned Blockchain is useful for Halal Supply Chain not just because it can secure transactions from some of the halal issues, but the transaction speed and rate to transfer data are very effective.
SARS-CoV-2: vaccines in the pandemic era
Authors: Li Dan-Dan , Li Qi-Han 
Published: January 2021

AbstractCoronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused millions of infections and deaths worldwide since its emergence in December 2019. As there is little or no natural immunity in the human population or specific anti-COVID-19 drugs, researchers from the government, academia and industry are developing vaccines at an unprecedented speed to halt the pandemic. In this review, the results of animal experiments and clinical trials on several vaccine technical platforms are summarized, and several challenges are also discussed to further promote the development, evaluation and application of vaccines during the challenging situation of the global pandemic.
Recurrence after ESD curative resection for early gastric cancer
Authors: Kamiya Ayako , Katai Hitoshi , Ishizu Kenichi , Wada Takeyuki , Hayashi Tsutomu , Otsuki Sho , Yamagata Yukinori , Yoshikawa Takaki , Sekine Shigeki , Nishi Tomohiko , Kawasaki Yuka , Ito Takafumi , Domoto Hideharu 
Published: January 2021

Abstract
Background
Endoscopic submucosal dissection (ESD) is gaining ground as a minimally invasive treatment for early gastric cancer (EGC) that has a negligible risk of lymph node metastasis. According to the 5th edition of Japanese gastric cancer treatment guidelines, annual or biannual follow-up with endoscopy is recommended, but follow-up with abdominal ultrasonography or computed tomography (CT) for surveillance of metastases is not recommended after the eCuraA resection. However, we experienced a case of lymph node recurrence following ESD resulting in eCuraA.

Case presentation
A 76-year-old female received ESD for EGC in a previous hospital 4 years ago. Pathological findings were tub1, 30 mm, T1a (M), UL0, Ly0, V0, pHM-, pVM- (eCuraA) according to the 15th edition of Japanese Classification of Gastric Carcinoma. Follow-up esophagogastroduodenoscopy revealed submucosal tumor, which was suspected as a swollen lymph node by CT and endoscopic ultrasound fine-needle aspiration revealed the recurrence of gastric cancer. We performed total gastrectomy with D2 lymph node dissection. Postoperative pathological examination revealed no local recurrent tumor at the ESD site in the stomach. Swollen lymph node was diagnosed as metastasis and lymph node metastasis was limited near the cardia.

Conclusion
This case provides valuable information about tumor with a minimum poorly differentiated adenocarcinoma component may develop lymph node metastasis even satisfying the guidelines criteria for curative resection.
Arctigenin attenuates CCl4-induced hepatotoxicity through suppressing matrix metalloproteinase-2 and oxidative stress
Authors: Kanawati Ghalia Mohamed , Al-Khateeb Iqbal Hassan , Kandil Yasser Ibrahim 
Published: January 2021

Abstract
Background
In spite of the huge advances in recent medicine, there is no effective drug that completely protects the liver from toxic materials. This study was conducted to investigate the hepatoprotective effect of arctigenin from burdock (Arctium lappa) against carbon tetrachloride (CCl4)-induced liver injury.

Results
Arctigenin pre-administration reduced hepatotoxicity markers significantly as compared to CCl4 group. In addition, both silymarin and arctigenin declined matrix metalloproteinase-2 (MMP-2) in the serum (1177 ± 176), (978 ± 135) significantly as compared to CCl4 group (1734 ± 294). The hepatic antioxidant parameters (total glutathione, superoxide dismutase, and glutathione reductase) were significantly decreased after CCl4 injection, an effect that has been prevented by pre-administration of both silymarin and arctigenin. Histological examinations illustrated that arctigenin reduced CCl4 damage, where it decreased inflammation, congestion, and ballooning.

Conclusions
Arctigenin exerted a hepatoprotective effect against CCl4-induced liver damage in terms of suppressing MMP-2 and oxidative stress comparative to that of silymarin.
High performance IIR filter implementation on FPGA
Authors: Datta Debarshi , Dutta Himadri Sekhar 
Published: January 2021

AbstractThis paper presents an improved design of reconfigurable infinite impulse response (IIR) filter that can be widely used in real-time applications. The proposed IIR design is realized by parallel–pipeline-based finite impulse response (FIR) filter. The FIR filters have excellent characteristics such as high stability, linear phase response and fewer finite precision errors. Hence, FIR-based IIR design is more attractive and selective in signal processing. In addition, the other two modern techniques such as look-ahead and two-level pipeline IIR filter designs are also discussed. All the said designs have been described in hardware description language and tested on Xilinx Virtex-5 field programmable gate array board. The implementation results show that the proposed FIR-based IIR design yields better performance in terms of hardware utilization, higher operating speed and lower power consumption compared to conventional IIR filter.
A rare case of paradoxical pulmonary embolism in spontaneous aortocaval fistula
Authors: Vespro Valentina , Fusco Stefano , Ierardi Anna Maria , Grassi Viviana , D’Alessio Ilenia , Crespi Silvia , Andrisani Maria Carmela , Bellobuono Andrea , Trimarchi Santi , Carrafiello Gianpaolo 
Published: January 2021

Aortocaval fistula (ACF) is a rare complication of abdominal aortic aneurysm (AAA), occurring in less than 1% of all AAAs. Paradoxical embolism can rarely be associated with ACF, pulmonary embolism may originate from dislodgment of thrombotic material from the AAA in the inferior vena cava (IVC) through the ACF. We report a case of a patient admitted to the emergency department with abdominal pain and shortness of breath who immediately underwent thoraco-abdominal CT. Imaging allowed a prompt pre-operative diagnosis of an ACF between an AAA and the IVC, also identifying CT signs of right heart overload and the presence of a paradoxical pulmonary embolism.
Beep or alert: evaluating WhatsApp vs pagers for communication between hospital staff
Authors: Baqai Muhammad WS , Hani Ummey , Shahzad Noman , Alvi Rehman 
Published: January 2021

Background/Aims A timely and effective communication system between hospital staff is vital for teamwork, patient safety and optimal clinical outcomes. This study compared staff perceptions of WhatsApp compared to traditional pagers for clinical communication in one of the largest tertiary care hospitals in Pakistan. Methods A questionnaire regarding factors such as the ease of use and reliability of each communication modality was distributed to interns and residents at the Aga Khan University Hospital in Karachi, Pakistan. Descriptive statistics were computed for percentages, frequencies and statistical significance of categorical variables. Results A total of 163 participants completed the survey. Of these, 95.1% preferred WhatsApp for storing information for later reference, 58.6% considered WhatsApp to be faster in terms of response, and 52.1% found WhatsApp easier to use than pagers. However, 60.7% chose pagers as better suited for emergency situations and 92.6% were concerned that WhatsApp could potentially compromise patient data security. Overall, participants preferred the development of a bespoke app for hospital communications instead. Conclusions WhatsApp was regarded as easier to use and more accessible than pagers, but it was not deemed to be compliant with hospital policies. Productive communication could be optimised with the use of a tailor-made app dedicated solely to clinical communication.