bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barriers and Facilitators to Integrating Behavioral Health Services and Pediatric Primary Care
Authors: Brady Keri J. S. , Durham Michelle P. , Francoeur Alex , Henneberg Cameron , Adhia Avanti , Morley Debra , Tamene Mahader , Singerman Amanda , Morris Anita , Fortuna Lisa R. , Feinberg Emily , Bair-Merritt Megan 
Published: November 2020

Objective: To describe the barriers and facilitators to integrating behavioral health services and pediatric primary care in federally qualified health centers (FQHCs) during the early stages of implementation. Method: We conducted 34 semistructured interviews with primary care providers ( n = 11), behavioral health clinicians ( n = 12), community health workers, and other pediatric staff ( n = 11) at 3 FQHCs. Themes were identified inductively using methods informed by grounded theory; inductively identified themes were then deductively organized within the Consolidated Framework for Implementation Research. Results: Interviewees perceived that the adoption and sustainability of behavioral health integration (BHI) in the pediatric practices of FQHCs were most dependent on barriers and facilitators in the outer setting (the health system context, including financing, partnerships with community organizations and providers, local supply of specialty behavioral health providers, and characteristics of their patient population) and internal clinic structures (resources to support protected provider/staff time, colocation, professional development, and adequate staffing). In turn, adequate clinic structure was perceived as a foundational component in facilitating the process, relational, and individual changes required for BHI implementation, including improving provider and staff collaboration and communication, reducing staff stigma, improving provider compassion, and supporting provider and staff well-being. Conclusions: The successful adoption and sustainability of BHI in the pediatric primary care practices of urban FQHCs may depend highly on the health system context and internal clinic structures. Implications for implementing pediatric BHI interventions in FQHCs are discussed. Implications for Impact Statement The findings of this research suggest that the successful adoption and sustainability of behavioral health integration (BHI) in the pediatric primary care practices of urban federally qualified health centers (FQHCs) may be most impacted by the health system context (reimbursement policies and practices, partnerships with community organizations and providers, the local supply of specialty behavioral health providers, and characteristics of their patient population) and internal clinic structures (resources to support protected provider/staff time, colocation, professional development, and adequate staffing). Our findings can be used by pediatric psychologists, health care professionals, administrators, and health policymakers to inform the design of future pediatric BHI interventions within urban FQHCs.
Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity
Authors: Zhou Lisha , Zhu Jin , Chen Wangyang , Jiang Yanyu , Hu Tao , Wang Yinxia , Ye Xiaoling , Zhan Mengxi , Ji Chenghao , Xu Zhuoming , Wang Xinran , Gu Yuanlong , Jia Lijun 
Published: November 2020

AbstractPlatinum is a widely used first-line chemotherapy in treating non-small cell lung cancer of adenocarcinoma. Unfortunately, platinum resistance leads to relapse and therapeutic failure, enabling the development of platinum-sensitization strategies to be of great clinical significance. Here, we report that the upregulation of the NEDD8-conjugating enzyme UBE2F is an important way for lung cancer cells to escape platinum-induced cell apoptosis, which confers to insensitivity to platinum-based chemotherapy. Mechanistically, platinum treatment impairs the complex formation for proteasome-mediated UBE2F degradation, evidenced by the weaker association between UBE2F and Ring-box protein 1 (RBX1), an essential component of Cullin-Ring E3 ligases (CRLs), thus leading to the accumulation of UBE2F. The accumulated UBE2F promotes the neddylation levels and activity of Cullin5, in accord with the lower expression of pro-apoptotic protein NOXA, a well-known substrate of Cullin-Ring E3 ligase 5 (CRL5). Additionally, knockout of UBE2F significantly sensitizes lung cancer cells to platinum treatment by enhancing the protein levels of NOXA and subsequently promoting cell apoptosis. Our observations uncover a previously unknown regulatory mechanism of UBE2F stability upon platinum chemotherapy and suggest that UBE2F might be a novel therapy target for sensitizing lung cancer cells to platinum-based chemotherapy.
3D-printed cellular tips for tuning fork atomic force microscopy in shear mode
Authors: Sun Liangdong , Gu Hongcheng , Liu Xiaojiang , Ni Haibin , Li Qiwei , Zeng Yi , Chang Ning , Zhang Di , Chen Hongyuan , Li Zhiyong , Zhao Xiangwei , Gu Zhongze 
Published: November 2020

AbstractConventional atomic force microscopy (AFM) tips have remained largely unchanged in nanomachining processes, constituent materials, and microstructural constructions for decades, which limits the measurement performance based on force-sensing feedbacks. In order to save the scanning images from distortions due to excessive mechanical interactions in the intermittent shear-mode contact between scanning tips and sample, we propose the application of controlled microstructural architectured material to construct AFM tips by exploiting material-related energy-absorbing behavior in response to the tip–sample impact, leading to visual promotions of imaging quality. Evidenced by numerical analysis of compressive responses and practical scanning tests on various samples, the essential scanning functionality and the unique contribution of the cellular buffer layer to imaging optimization are strongly proved. This approach opens new avenues towards the specific applications of cellular solids in the energy-absorption field and sheds light on novel AFM studies based on 3D-printed tips possessing exotic properties.
Pharmacological targeting of MCL-1 promotes mitophagy and improves disease pathologies in an Alzheimer’s disease mouse model
Authors: Cen Xufeng , Chen Yanying , Xu Xiaoyan , Wu Ronghai , He Fusheng , Zhao Qingwei , Sun Qiming , Yi Cong , Wu Jie , Najafov Ayaz , Xia Hongguang 
Published: November 2020

AbstractThere is increasing evidence that inducing neuronal mitophagy can be used as a therapeutic intervention for Alzheimer’s disease. Here, we screen a library of 2024 FDA-approved drugs or drug candidates, revealing UMI-77 as an unexpected mitophagy activator. UMI-77 is an established BH3-mimetic for MCL-1 and was developed to induce apoptosis in cancer cells. We found that at sub-lethal doses, UMI-77 potently induces mitophagy, independent of apoptosis. Our mechanistic studies discovered that MCL-1 is a mitophagy receptor and directly binds to LC3A. Finally, we found that UMI-77 can induce mitophagy in vivo and that it effectively reverses molecular and behavioral phenotypes in the APP/PS1 mouse model of Alzheimer’s disease. Our findings shed light on the mechanisms of mitophagy, reveal that MCL-1 is a mitophagy receptor that can be targeted to induce mitophagy, and identify MCL-1 as a drug target for therapeutic intervention in Alzheimer’s disease.
The thermal response of soil microbial methanogenesis decreases in magnitude with changing temperature
Authors: Chen Hongyang , Zhu Ting , Li Bo , Fang Changming , Nie Ming 
Published: November 2020

AbstractMicrobial methanogenesis in anaerobic soils contributes greatly to global methane (CH4) release, and understanding its response to temperature is fundamental to predicting the feedback between this potent greenhouse gas and climate change. A compensatory thermal response in microbial activity over time can reduce the response of respiratory carbon (C) release to temperature change, as shown for carbon dioxide (CO2) in aerobic soils. However, whether microbial methanogenesis also shows a compensatory response to temperature change remains unknown. Here, we used anaerobic wetland soils from the Greater Khingan Range and the Tibetan Plateau to investigate how 160 days of experimental warming (+4°C) and cooling (−4°C) affect the thermal response of microbial CH4respiration and whether these responses correspond to changes in microbial community dynamics. The mass-specific CH4respiration rates of methanogens decreased with warming and increased with cooling, suggesting that microbial methanogenesis exhibited compensatory responses to temperature changes. Furthermore, changes in the species composition of methanogenic community under warming and cooling largely explained the compensatory response in the soils. The stimulatory effect of climate warming on soil microbe-driven CH4emissions may thus be smaller than that currently predicted, with important consequences for atmospheric CH4concentrations.
Quantitative and multiplexed chemical-genetic phenotyping in mammalian cells with QMAP-Seq
Authors: Brockway Sonia , Wang Geng , Jackson Jasen M. , Amici David R. , Takagishi Seesha R. , Clutter Matthew R. , Bartom Elizabeth T. , Mendillo Marc L. 
Published: November 2020

AbstractChemical-genetic interaction profiling in model organisms has proven powerful in providing insights into compound mechanism of action and gene function. However, identifying chemical-genetic interactions in mammalian systems has been limited to low-throughput or computational methods. Here, we develop Quantitative and Multiplexed Analysis of Phenotype by Sequencing (QMAP-Seq), which leverages next-generation sequencing for pooled high-throughput chemical-genetic profiling. We apply QMAP-Seq to investigate how cellular stress response factors affect therapeutic response in cancer. Using minimal automation, we treat pools of 60 cell types—comprising 12 genetic perturbations in five cell lines—with 1440 compound-dose combinations, generating 86,400 chemical-genetic measurements. QMAP-Seq produces precise and accurate quantitative measures of acute drug response comparable to gold standard assays, but with increased throughput at lower cost. Moreover, QMAP-Seq reveals clinically actionable drug vulnerabilities and functional relationships involving these stress response factors, many of which are activated in cancer. Thus, QMAP-Seq provides a broadly accessible and scalable strategy for chemical-genetic profiling in mammalian cells.
Controllable modulation of precursor reactivity using chemical additives for systematic synthesis of high-quality quantum dots
Authors: Park Joonhyuck , Jayaraman Arun , Schrader Alex W. , Hwang Gyu Weon , Han Hee-Sun 
Published: November 2020

AbstractThe optical and electronic performance of quantum dots (QDs) are affected by their size distribution and structural quality. Although the synthetic strategies for size control are well established and widely applicable to various QD systems, the structural characteristics of QDs, such as morphology and crystallinity, are tuned mostly by trial and error in a material-specific manner. Here, we show that reaction temperature and precursor reactivity, the two parameters governing the surface-reaction kinetics during growth, govern the structural quality of QDs. For conventional precursors, their reactivity is determined by their chemical structure. Therefore, a variation of precursor reactivity requires the synthesis of different precursor molecules. As a result, existing precursor selections often have significant gaps in reactivity or require synthesis of precursor libraries comprising a large number of variants. We designed a sulfur precursor employing a boron-sulfur bond, which enables controllable modulation of their reactivity using commercially available Lewis bases. This precursor chemistry allows systematic optimization of the reaction temperature and precursor reactivity using a single precursor and grows high-quality QDs from cores of various sizes and materials. This work provides critical insights into the nanoparticle growth process and precursor designs, enabling the systematic preparation of high-quality QD of any sizes and materials.
Genomic analysis of Bacillus cereus NWUAB01 and its heavy metal removal from polluted soil
Authors: Ayangbenro Ayansina Segun , Babalola Olubukola Oluranti 
Published: November 2020

AbstractMicroorganisms that display unique biotechnological characteristics are usually selected for industrial applications. Bacillus cereus NWUAB01 was isolated from a mining soil and its heavy metal resistance was determined on Luria–Bertani agar. The biosurfactant production was determined by screening methods such as drop collapse, emulsification and surface tension measurement. The biosurfactant produced was evaluated for metal removal (100 mg/L of each metal) from contaminated soil. The genome of the organism was sequenced using Illumina Miseq platform. Strain NWUAB01 tolerated 200 mg/L of Cd and Cr, and was also tolerant to 1000 mg/L of Pb. The biosurfactant was characterised as a lipopeptide with a metal-complexing property. The biosurfactant had a surface tension of 39.5 mN/m with metal removal efficiency of 69%, 54% and 43% for Pb, Cd and Cr respectively. The genome revealed genes responsible for metal transport/resistance and biosynthetic gene clusters involved in the synthesis of various secondary metabolites. Putative genes for transport/resistance to cadmium, chromium, copper, arsenic, lead and zinc were present in the genome. Genes responsible for biopolymer synthesis were also present in the genome. This study highlights biosurfactant production and heavy metal removal of strain NWUAB01 that can be harnessed for biotechnological applications.
Spontaneous brain state oscillation is associated with self-reported anxiety in a non-clinical sample
Authors: Qiao Lei , Luo Xi , Zhang Lijie , Chen Antao , Li Hong , Qiu Jiang 
Published: November 2020

AbstractThe anti-correlation relationship between the default-mode network (DMN) and task-positive network (TPN) may provide valuable information on cognitive functions and mental disorders. Moreover, maintaining a specific brain state and efficaciously switching between different states are considered important for self-regulation and adaptation to changing environments. However, it is currently unclear whether competitions between the DMN and TPN are associated with negative affect (here, anxiety and depression) in non-clinical samples. We measured the average dwell time of DMN dominance over the TPN (i.e., the average state duration before transition to another state, indicating persistent DMN dominance) with a sample of 302 non-clinical young adults. Subsequently, we explored individual differences in this persistent DMN dominance by examining its correlations with subjective depression and anxiety feelings. Moreover, we linked state transition between DMN/TPN dominance with right fronto-insular cortex (RFIC) blood oxygen-level dependent signal variability. We found that the average dwell time of DMN dominance was positively associated with self-reported anxiety. Furthermore, state transition between DMN or TPN dominance was positively linked to RFIC activity. These findings highlight the importance of investigating the complex and dynamic reciprocal inhibition patterns of the DMN and TPN and the important role of the RFIC in the association between these networks.
Molecular nature of breakdown of the folic acid under hydrothermal treatment: a combined experimental and DFT study
Authors: Abramova Anna M. , Kokorina Alina A. , Sindeeva Olga A. , Jolibois Franck , Puech Pascal , Sukhorukov Gleb B. , Goryacheva Irina Y. , Sapelkin Andrei V. 
Published: November 2020

AbstractUsing a combination of experimental Raman, FTIR, UV–VIS absorption and emission data, together with the corresponding DFT calculations we propose the mechanism of modification of the folic acid specifically under the hydrothermal treatment at 200 °C. We established that folic acid breaks down into fragments while the pteridine moiety remains intact likely evolving into 6-formylpterin with the latter responsible for the increase in fluorescence emission at 450 nm. The results suggest that hydrothermal approach can be used for production of other purpose-engineered fluorophores.
Extent and prognostic value of MGMT promotor methylation in glioma WHO grade II
Authors: Karschnia Philipp , Teske Nico , Dorostkar Mario M. , Siller Sebastian , Weller Jonathan , Baehring Joachim M. , Dietrich Jorg , von Baumgarten Louisa , Herms Jochen , Tonn Joerg-Christian , Thon Niklas 
Published: November 2020

AbstractMGMT promotor methylation is associated with favourable outcome in high-grade glioma. In glioma WHO grade II, it is unclear whether the extent of MGMT promotor methylation and its prognostic role is independent from other molecular markers. We performed a retrospective analysis of 155 patients with glioma WHO grade II. First, all 155 patients were assigned to three molecular groups according to the 2016 WHO classification system: (1) oligodendroglioma, IDH-mutant and 1p19q co-deleted (n = 81); (2) astrocytoma, IDH-mutant and 1p19q non-codeleted (n = 54); (3) astrocytoma, IDH-wildtype (n = 20). MGMT promotor methylation was quantified using Sanger sequencing of the CpG sites 74–98 within the MGMT promotor region. Highest numbers of methylated CpG sites were found for oligodendroglioma, IDH-mutant and 1p19q co-deleted. When 1p19q co-deletion was absent, numbers of methylated CpG sites were higher in the presence of IDH-mutation. Accordingly, lowest numbers were seen in the IDH-wildtype subpopulation. In the entire cohort, larger numbers of methylated CpG sites were associated with favourable outcome. When analysed separately for the three WHO subgroups, a similar association was only retained in astrocytoma, IDH-wildtype. Collectively, extent of MGMT promotor methylation was strongly associated with other molecular markers and added prognostic information in astrocytoma, IDH-wildtype. Evaluation in prospective cohorts is warranted.
Robustly forecasting maize yields in Tanzania based on climatic predictors
Authors: Laudien Rahel , Schauberger Bernhard , Makowski David , Gornott Christoph 
Published: November 2020

AbstractSeasonal yield forecasts are important to support agricultural development programs and can contribute to improved food security in developing countries. Despite their importance, no operational forecasting system on sub-national level is yet in place in Tanzania. We develop a statistical maize yield forecast based on regional yield statistics in Tanzania and climatic predictors, covering the period 2009–2019. We forecast both yield anomalies and absolute yields at the sub-national scale about 6 weeks before the harvest. The forecasted yield anomalies (absolute yields) have a median Nash–Sutcliffe efficiency coefficient of 0.72 (0.79) in the out-of-sample cross validation, which corresponds to a median root mean squared error of 0.13 t/ha for absolute yields. In addition, we perform an out-of-sample variable selection and produce completely independent yield forecasts for the harvest year 2019. Our study is potentially applicable to other countries with short time series of yield data and inaccessible or low quality weather data due to the usage of only global climate data and a strict and transparent assessment of the forecasting skill.
EGFP-EGF1-conjugated poly (lactic-co-glycolic acid) nanoparticles as a carrier for the delivery of CCR2− shRNA to atherosclerotic macrophage in vitro
Authors: Wu Zhilin , Chen Chen , Luo Jiajia , Davis Jacques R. J. , Zhang Bo , Tang Liang , Shi Wei , Liao Danying 
Published: November 2020

AbstractReducing macrophage recruitment by silencing chemokine (C–C motif) receptor 2 (CCR2) expression is a promising therapeutic approach against atherosclerosis. However the transfection of macrophages with siRNA is often technically challenging. EGFP-EGF1-conjugated poly (lactic-co-glycolic acid) (PLGA) nanoparticles (ENPs) have a specific affinity to tissue factor (TF). In this study, the feasibility of ENPs as a carrier for target delivery of CCR2-shRNA to atherosclerotic cellular models of macrophages was investigated. Coumarin-6 loaded ENPs were synthesized using a double-emulsion method. Fluorescence microscopy and flow cytometry assay were taken to examine the uptake of Coumarin-6 loaded ENPs in the cellular model. Then a sequence of shRNA specific to CCR2 mRNA was constructed and encapsulated into ENPs. Target delivery of CCR2-shRNA to atherosclerotic cellular models of macrophages in vitro were evaluated. Results showed more uptake of ENPs by the cellular model than common PLGA nanoparticles. CCR2-shRNA loaded ENPs effectively silenced CCR2 gene in the atherosclerotic macrophages and exhibited a favorable cytotoxic profile to cultured cells. With their low cytotoxicity and efficient drug delivery, ENP could be a useful carrier for target delivery of CCR2-shRNA to inflammatory monocytes/macrophages for the therapy against atherosclerosis.
Long-term washover fan accretion on a transgressive barrier island challenges the assumption that paleotempestites represent individual tropical cyclones
Authors: Rodriguez Antonio B. , Theuerkauf Ethan J. , Ridge Justin T. , VanDusen Beth M. , Fegley Stephen R. 
Published: November 2020

AbstractBarrier island overwash occurs when the elevation of wave runup exceeds the dune crest and induces landward transport of sediment across a barrier island and deposition of a washover deposit. Washover deposition is generally attributed to major storms, is important for the maintenance of barrier island resilience to sea-level rise and is used to extend hurricane records beyond historical accounts by reconstructing the frequency and extent of washover deposits preserved in the sedimentary record. Here, we present a high-fidelity 3-year record of washover evolution and overwash at a transgressive barrier island site. During the first year after establishment, washover volume and area increased 1595% and 197%, respectively, from at least monthly overwash. Most of the washover accretion resulted from the site morphology having a low resistance to overwash, as opposed to being directly impacted by major storms. Washover deposits can accrete landward over multi-year time scales in the absence of large storms; therefore, paleotempestites can be more complex than single event beds.
Physiological synchrony is associated with cooperative success in real-life interactions
Authors: Behrens F. , Snijdewint J. A. , Moulder R. G. , Prochazkova E. , Sjak-Shie E. E. , Boker S. M. , Kret M. E. 
Published: November 2020

AbstractCooperation is pivotal for society to flourish. To foster cooperation, humans express and read intentions via explicit signals and subtle reflections of arousal visible in the face. Evidence is accumulating that humans synchronize these nonverbal expressions and the physiological mechanisms underlying them, potentially influencing cooperation. The current study is designed to verify this putative linkage between synchrony and cooperation. To that end, 152 participants played the Prisoner’s Dilemma game in a dyadic interaction setting, sometimes facing each other and sometimes not. Results showed that synchrony in both heart rate and skin conductance level emerged during face-to-face contact. However, only synchrony in skin conductance levels predicted cooperative success of dyads. Crucially, this positive linkage was strengthened when participants could see each other. These findings show the strong relationship between our bodily responses and social behavior, and emphasize the importance of studying social processes between rather than within individuals in real-life interactions.