bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Different Anaesthesia Methods on Perioperative Cellular Immune Function and Long-term Outcomes in Patients Undergoing Radical Resection of Esophageal Cancer: A Prospective Cohort Study
Authors: Cong Xuhui , Zhang Wei , Huang Zhoufeng , Zhang Liyuan , Sun Mingyang , Chang Enqiang , Zhang Jiaqiang 
Published: July 2020

Abstract
Background The present study aimed to analyse the effects of different an anaesthetic method on perioperative cellular immunity and long-term outcomes in patients having undergone oesophageal cancer surgery.Design A Prospective Cohort StudyParticipants 120 cases of patients with oesophageal cancer in the Zhengzhou University People’s Hospital from January 2016 to January 2017 were recruited and randomly split into GA group (general anaesthesia, n=40), PG group (Paravertebral nerve blocks with general anaesthesia, n = 40) and EG group (epidural anaesthesia with general anaesthesia, n=40). Methods Self-rating anxiety scale and visual analogue scale scores were adopted to compare postoperative anxiety and the degree of pain of patients of the three groups. Besides, adverse reactions of patients in the three groups were compared. Pre-operation, the end of operation, postoperative day (POD) 1 and POD 2 levels of interleukin-6 (IL-6), IL-4, tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and the survival of T-cell subsets (CD3+, CD4+, CD8+, CD4+/CD8+) were measured with by either ELISA or flow cytometry.Results In the PG or EG group, VAS scores were lower, and fewer opioids and vasoactive agents were used, as compared with those of the GA group. In both the EG and PG groups, higher CD3+ and CD4+ cell survival and lower levels of Cor, IL-4 and IL-6 were identified at the end of or after the surgery than those in the GA group. Moreover, the postoperative survival cure of the PG and EG groups was better than that of the GA group.Conclusions The combination of paravertebral nerve blocks or epidural anaesthesia and general anaesthesia may improve the perioperative immune function and the long-term outcome for patients having undergone oesophageal cancer surgery.
Persistently higher serum sCD40L levels are associated with mortality in septic patients
Authors: Liang Yingjian , Zhu Chengrui , Sun Yini , Li Zhiliang , Wang Liang , Liu Yina , Li Xin , ma xiaochun 
Published: July 2020

Abstract
Background
Soluble CD40 ligand (sCD40L) exhibits proinflammatory and procoagulant effects. Recent data indicated that sCD40L plays a significant role in septic patients. The aim of the present study was to determine sCD40L changes in surgical patients without sepsis (SWS) and in surgical sepsis patients (SS) during the first three days at Intensive Care Unit (ICU) admission, and to observe the association between sCD40L and mortality.
Methods
Time changes in sCD40L levels were assessed for 3 days after ICU admission in 49 patients with SS and compared with 19 SWS. Serum sCD40L concentration was detected by ELISA. Survival at 28-days was used as the endpoint.
Results
SS had significantly higher sCD40L levels than SWS and control patients. Advanced age (P = 0.023) was observed in the group of nonsurviving patients compared with surviving SS. We observed an association between sCD40L levels ≥ 1028.75 pg/ml at day 2 and 28-days mortality (odds ratio = 7.888; 95% confidence interval = 1.758 to 35.395; P = 0.007).
Conclusions
Septic patients show persistently higher circulating sCD40L levels in the first three days at ICU admission, and it is likely that sCD40L on the day 2 may have a predictive value; thus, serum sCD40L could be used as a reliable biomarker and therapeutic target in sepsis.
Effects of Total Intravenous Anesthesia with Propofol Versus Inhalational Anesthesia with Sevoflurane on Postoperative Quality of Life and Postoperative Delirium in Patients Undergoing Total Knee Arthroplasty: A Randomized, Controlled, Double-Blinded Clinical Trial
Authors: Su Han , Fang Yuxin , Xu Guanghong , Liu Xuesheng 
Published: July 2020

Abstract
Background: The widespread use of total knee arthroplasty(TKA) to treat patients who suffer knee osteoarthritis(KOA) has led to a number of postoperative problems, including the recovery of postoperative quality of life and postoperative delirium(POD). Both problems could cause profound damages to patients and their families. This study assessed and compared the effects of total intravenous anesthesia with propofol versus inhalational anesthesia (sevoflurane) on postoperative quality of life and POD in patients who underwent TKA.Methods: One hundred and fifty patients American Society of Anesthesiologists(ASA) I-III were assessed for inclusion in this study, and randomly divided into inhalational anesthesia with sevoflurane group(group S), and total intravenous anesthesia with propofol group(group P). The primary outcome was postoperative quality of life and secondary outcome was POD.Results: The quality of life at the first month after surgery in group S was better than that in group P(P=0.02). However, there was no significant difference in quality of life between the two groups at 1 day before surgery, 1 day after surgery, 3 days after surgery, 7 days after surgery and 3 months after surgery. In group P, 19 patients (31.1%) had no anxiety/depression problems, 40 patients (65.6%) had moderate problems, and 2 patients (3.3%) had severe problems.In group S, 37 patients (60.6%) had no problems in anxiety/depression, 23 patients (37.7%) had moderate problems, and 1 patient had severe problems (1.7%). These two outcomes had significant differences (P=0.005) .The incidence of POD within 3 days after surgery was 24.6% (15/61) in group P and 36.1% (22/61) in group S. There was no significant difference in the incidence of POD between the two groups (P=0.17).Conclusions: Group S patients have a better quality of life at the first month after surgery in comparison with those in group P regarding anxiety/depression. However, there were no significant differences in the quality of lives between the two groups at 1 day before surgery, 1 day after surgery, 3 days after surgery, 7 days after surgery, and 3 months after surgery. There was also no significant difference in POD. Thus, we concluded that both anesthetic technologies could be extensively used for TKA.Trial registration: This trial was registered at the Chinese Clinical Trial Registry (ChiCRT-IOR-17012428). Date of Registration: 21 Aug. 2017.
CLTRN, regulated by NRF1/RAN/DLD protein complex, enhances radiation sensitivity of hepatocellular carcinoma cells through ferroptosis pathway
Authors: Yuan Yin , Cao Wen , Zhou Hongbing , Qian Haixin , Wang Honggang 
Published: July 2020

Abstract
Background
Radiotherapy is a viable treatment option for patients with unresectable hepatocellular carcinoma (HCC). However, radiation resistance is an issue that needs to be addressed. In this context, cumulative evidence supports the functional roles of a variety of RNA or proteins in radioresistance, and suggests that the modulation of their expression may constitute a novel radiosensitization approach. Here, we investigated the ability of collectrin (CLTRN) to enhance the radiosensitivity in HCC patients for the first time.
Methods
Transcriptome sequencing technology (RNA-seq technology) was used to analyze the transcription-level changes in the genes from the HepG2 cells before and after X-ray irradiation. Combining the results with the HCC tissue RNA-seq data, we determined the ultimate target gene through bioinformatics analysis and cellular verification. A series of cellular and molecular biology techniques were applied in vitro and in vivo to confirm whether CLTRN can enhance radiosensitivity in HCC cells. Subsequently, the downstream action mechanism, the upstream transcription factor, and the interaction proteins of CLTRN were determined.
Results
First, we confirmed the association between CLTRN and radiosensitivity. We observed that CLTRN overexpression led to a significant reduction in the proliferation, migration, and invasion potential of X-ray-irradiated HCC cells, whereas no observable effect was exerted on cell cycle and apoptosis. The same results were observed in nude mice in vivo. Investigation of the gene regulatory mechanism revealed that the genes analyzed at transcriptome level after CLTRN overexpression were mostly enriched in the glutathione metabolic pathway. As glutathione metabolism forms a vital link in ferroptosis, we surmised that CLTRN is associated with ferroptosis. This was confirmed through the detection of cellular iron, ROS level determination, transmission electron microscopy, and monitoring of ferroptosis-related protein indicators. Lastly, we investigated whether nuclear respiratory factor 1 (NRF1) is the upstream transcription factor of CLTRN, and whether dihydrolipoamide dehydrogenase (DLD) and members of the RAS oncogene family (RAN) are its interacting proteins.
Conclusion
Our results indicate that CLTRN is a vital regulator of radiation sensitivity and could serve as a novel therapeutic target or prognostic marker in HCC treatment.
lncRNA DLEU2 promotes gastric cancer progression through ETS2 via targeting miR-30a-5p
Authors: Han Shuyi , Xu Yihui , Wang Min , Wang Jun , Wang Jing , Yuan Mingjie , Jia Yanfei , Ma Xiaoli , Wang Yunshan , Liu Xiangdong 
Published: July 2020

Abstract
Background

Gastric cancer (GC) is the fourth common and the second lethal malignancy. Further understanding of the molecular mechanisms of underlying gastric carcinogenesis will enhance the diagnosis and treatment of GC.
Methods

The expression of lncRNA DLEU2 and ETS2 was analysed using GEPIA online analyze, qRT-PCR and immunohistochemistry. siRNAs targeting to lncRNA DLEU2 were designed to reduce the expression of lncRNA DLEU2. miR-30a-5p mimics were purchased to up-regulate the level of miR-30a-5p. The pcDNA3.1-ETS2 vector was synthesized to up-regulate the expression of ETS2. The biological function of GC cells was detected by CCK8, clone formation, transwell, wound healing, western blot, and flow cytometry assay. More in-depth mechanisms were studied.
Results

LncRNA DLEU2 was significantly up-regulated in GC tissues. The expression of lncRNA DLEU2 was significantly associated with pathological grading and TNM stage of GC patients. Furthermore, knockdown of lncRNA DLEU2 inhibited the proliferation, migration and invasion of AGS and MKN-45 cells, and induced cell apoptosis. We also found that miR-30a-5p could directly bind to the 3’ UTR region of ETS2. Moreover, lncRNA DLEU2 bound to miR-30a-5p through the same binding site, which facilitated the expression of ETS2. Knockdown of lncRNA DLEU2 reduced the protein level of intracellular ETS2 and inhibited the AKT phosphorylation. ETS2 was highly expressed in GC tissues. The expression of ETS2 was significantly associated with age, pathological grading and TNM stage. ETS2 overexpression promoted cell proliferation and migration of AGS and MKN-45 cells. Furthermore, ETS2 overexpression rescued cell proliferation and migration inhibition induced by lncRNA DLEU2 down-regulation and miR-30a-5p up-regulation in AGS and MKN-45 cells. These results showed that lncRNA DLEU2 may regulate GC cells process through miR-30a-5p/ETS2 axis. In addition, hypoxic microenvironment resulted in the accumulation and nucleation of HIF-1α in gastric cancer cells, and up-regulated the expression of DLEU2.
Conclusions

LncRNA DLEU2 was a potential molecular target for GC treatment.
Dihydroartemisinin Sensitizes Esophageal Squamous Cell Carcinoma to Cisplatin by Inhibiting Sonic Hedgehog Signaling
Authors: Cui Wei , Fang Tingting , Duan Zhaoheng , Xiang Dongfang , Wang Yanxia , Zhang Mengsi , Zhai Fangzheng , Cui Xiang , Yang Lang 
Published: July 2020

Abstract
Background: Platinum-based regimens have been routinely used in the clinical treatment of patients with esophageal squamous cell carcinoma (ESCC). However, administration of these drugs is frequently accompanied by drug resistance. Revealing the underlying mechanisms of the drug resistance and developing agents that enhance the sensitivity to platinum may provide new therapeutic strategies for the patients. Methods: Immunohistochemistry, western blotting, RT-PCR, flow cytometry and immunofluorescence microscopy were used to detect the expression of Shh pathway members and cancer stem cell(CSC) biomarkers in ESCC specimens and cell lines. Functional assays, including MTT, tumorsphere formation assay, RTCA and an in vivo tumour growth assay, were conducted to assess the effect of Dihydroartemisinin (DHA) on the proliferation and renewal ability of ESCC cells. HPLC was used to examine the concention of cisplatin in ESCC cells.Results: We found that the poor outcome of ESCC patients receiving platinum-based regimens was associated with co-expression of Shh and Sox2. The sensitivity of ESCC cell lines to cisplatin was related to their activity of Shh signaling. Manipulating of Shh expression markedly changed the sensitivity of ESCC cells to platinum. Continuous treatment with cisplatin resulted in the activation of Shh signaling and enhanced cancer stem cell-like phenotypes in ESCC cells. DHA, a classic antimalarial drug, was identified as a novel inhibitor of Shh pathway. Treatment with DHA attenuated the cisplatin-induced activation of the Shh pathway in ESCC cells and synergized the inhibitory effect of cisplatin on proliferation, sphere and colony formation of ALDH-positive ESCC cells in vitro and growth of ESCC cell-derived xenograft tumors in vivo. Conclusion: These results demonstrate that the Shh pathway is an important player in cisplatin-resistant ESCC and DHA acts as a promising therapeutic agent to sensitize ESCC to cisplatin treatment.
Airway Obstruction as the Primary Manifestation of Infantile Thyroid Hemangioma: A Case Report and Literature Review
Authors: Liang Yujian , Pu Ronghui , Huang Xueqiong , Li Suping , Chen Yingqian , Tang Wen 
Published: July 2020

Abstract
Background: Thyroid hemangioma mostly occurs in adults and is extremely rare in infants. So far, only four pediatric cases of thyroid hemangioma have been reported, one of which has only been clinically diagnosed. Most of the reported cases are of cavernous hemangiomas; capillary hemangioma cases are very rare. To date, there are no reports on capillary thyroid hemangioma in an infant. Therefore, here we present the case of an infant with a primary capillary hemangioma of the thyroid gland, and discuss its treatment and follow-up.Case Presentation: A 2-month-old infant with normal thyroid function presented with airway obstruction as the primary manifestation of thyroid hemangioma. The main symptoms were laryngeal wheezing and dyspnea. Ultrasonography revealed hypoechoic lesions at the lower pole of the bilateral thyroid. Computed tomography revealed enlargement of the thyroid gland, inhomogeneous parenchyma enhancement, and multiple thyroid nodules. However, these imaging modalities were unable to provide an exact diagnosis and the nature of the tumor remained unknown prior to an operation. Therefore, a postoperative histopathological examination was undertaken, which revealed capillary thyroid hemangioma. The symptoms significantly improved by a combined treatment involving surgery and oral propranolol (2 mg/kg per day).Conclusion: When a well-defined capsulate mass is detected on the medical image, the possibility of primary thyroid hemangioma must be considered.
Comparison of two techniques for the treatment for atlantoaxial instability injury: C1–C2 transarticular screws and C1 lateral mass–C2 pedicle screws
Authors: Du Hoang Gia , Thanh Dao Xuan , Phuoc Vu Xuan , Hoang Nguyen Duc , Tan Le Dang , Trung Nguyen Van 
Published: July 2020

Abstract
The authors have requested that this preprint be removed from Research Square.
Assessment of Immunization Data Quality of Routine Reports in Ho Municipality of Volta Region, Ghana
Authors: Asem Livingstone , Ziema Sorengmen Amos 
Published: July 2020

Abstract
Background: Immunization has been an important public health intervention for preventing and reducing child morbidity and mortality over the years and coverage has increased in the past decades. However, the validity of the data from immunization coverages is usually disputed. Immunization data from health facilities show poor concordance between tallied registers and monthly reports as they are reported to higher levels of the health system. The study assessed the quality of data from routine immunization of some health facilities in the Ho central municipality in the Volta region of Ghana.Methods: A descriptive cross-sectional study was used to review routine immunization data in tallied registers and reports submitted to the Municipal Health Directorate (MHD) from January to December, 2015. Simple random sampling was used to select three health facilities in Ho central municipality. The Data Quality Self-assessment tool (DQS) was the main instrument used to present and analyze data for accuracy and discrepancy level between the tallied registers and reports. A template was created in Microsoft excel which automatically presented accuracy and discrepancy levels when data was entered. Ethical approval for the study was obtained from Ghana Health Service Ethics Review Committee. Results: The result showed discrepancies between recounted tallies at the facilities and reports submitted to the MHD. Accuracy ratios of 102%, 64% and 94% for Bacillus Calmette Guerin (BCG), Pentavalent (Penta) vaccine dose 3 and Measles 2 respectively indicating underreporting for BCG and over reporting for the rest were obtained. There was 460 over reported data to the municipal level representing accuracy ratio of 80% and discrepancy level of 20%. Conclusions: Immunization data was characterized by underreporting and overreporting, hence not accurate and lacked quality. Immunization data quality should be a priority among health staff at health facilities.
Unlike Morphine, Long-Term Exposure to Analgesic Mitragynine, 7-Hydroxymitragynine, Paynantheine, and Speciociliatine Alkaloids Does Not Contribute to Antinociceptive Tolerance of μ-Opioid Receptors
Authors: Elahian Fatemeh , Zahedian Sorour , Safaei Mohsen , Pahlevani-Gazi Elham , Mirzaei Seyed Abbas 
Published: July 2020

Abstract
Background: Opioids are the most well known antinociceptive alkaloids all over the world, but tolerance and physical dependence are their dominant concerns in clinical applications. In contrast, monoterpene alkaloids are newly considered for their roles in pain management.Methods: In this regard, the clinical safety of mitragynine, 7-hydroxymitragynine, speciociliatine, and paynantheine was determined by cytotoxicity, antioxidant, and antigenotoxicity assays. In parallel alkaloids were studied on β-arrestin-2, ERK1/2, and p-ERK1/2 transcription and translation levels during three days on SH-SY5Y cells. Results: Results confirmed alkaloid- and concentration- dependency of the cytotoxicity, antigenotoxicity, and antioxidant activity. Although morphine was recorded as the safest alkaloid here, speciociliatine and mitragynine represented around 1200- and 20- fold higher DNA protection capacity than morphine, respectively. Monoterpene alkaloids transiently made the transcript ocean turbulent, but western blot analyses have proved significant down-regulation of targeted proteins in SH-SY5Y cells during the experiment days. Conclusions: Data indicated that monoterpene alkaloid derivatives are putative analgesic agents that do not stimulate opioid receptor tolerance and suggesting that patients may benefit from their antinociceptive activities without physical dependence or tolerance concerns in future clinics.
Synergistic effect of Vitamin D Supplement and mindfulness on insulin resistance and different aspects of cognitive function in Vitamin D Deficient Type 2 Diabetic mellitus (T2DM) women: A pilot randomized clinical trial with placebo-controlled
Authors: davoudi mohammadreza , Rezaei Parnian , Ahmadi Seyyed Mojtaba , Taheri Amir Abbas 
Published: July 2020

Abstract
Purpose

This study aimed to examining Synergistic effect of Vitamin D (VD) Supplement and mindfulness on insulin resistance and different aspects of cognitive function in VD Deficient Type 2 Diabetic women.
Methods

In this randomized-controlled trial, totally 225 women with T2DM and VD deficiency were randomly allocated to five groups: (1) mindfulness, (2) mindfulness and VD, (3) mindfulness and placebo, (4) VD, and (5) placebo. Mindfulness includes 12 sessions and VD patients received a daily 4000 IU oral dosage (two capsules) with 28,000 IU vitamin D weekly for 12 weeks. Sun exposure, anthropometric indices, dietary intake, physical activity, energy levels, laboratory analyses, were measured at the pre-test and after 12-week supplementation. dependent variables include cognitive function (Risky decision making, IQ and Trail Making Test) and insulin resistance.
Results

In baseline, measures were not different among the groups. At the end of treatment, cognitive function results showed improvement for all groups except the “placebo” group. About other groups, there was not any difference between VD and mindfulness groups (or combinations with placebo). but in “VITAMIN + MINDFULNESS” has a greater improvement rather than other groups (P < 0.05). This result exactly repeated for insulin resistance. However, there were not any significant difference among groups for IQ(P > 0.05). There was any difference among groups for sunlight exposure and BMI (P > 0.05). For FBS only in combined group (VD + Mindfulness) the FBS significantly reduced that is synergistic effects (P < 0.05).
Conclusion

Combining VD Supplement and mindfulness can reduce insulin resistance and improve different aspects of cognitive function in VD Deficient patients.
Haemolysis in Patients with End-Stage Renal Disease Receiving Haemodialysis: A Cross-Sectional Study
Authors: Matsumura Koichiro , Okumiya Toshika , Sugiura Tetsuro , Takahashi Nobuyuki , Yamamoto Yoshihiro , Kikuchi Sanae , Fujii Kenichi , Otagaki Munemitsu , Shiojima Ichiro 
Published: July 2020

Abstract
Background: Haemolysis due to mechanical stress on red blood cells (RBCs) during extracorporeal circulation has been reported in patients receiving haemodialysis. However, little is known about the incidence of, and the mechanisms underlying haemolysis in patients receiving maintenance haemodialysis. We sought to investigate the incidence and underlying mechanisms of haemolysis in patients with end-stage renal disease undergoing maintenance dialysis by measuring erythrocyte creatine.Method: In a cross-sectional study, we evaluated 69 patients with end-stage renal disease receiving haemodialysis (n = 55) or peritoneal dialysis (n = 14). Erythrocyte creatine, a quantitative marker of mean RBC age, was measured with other haemolytic markers including haptoglobin, reticulocyte count and lactate dehydrogenase.Results: RBC age was significantly shorter [47.7 (43.2–52.8) days vs. 59.8 (56.0–66.2) days, p<0.0001], and haptoglobin was significantly lower in haemodialysis patients than in those with peritoneal dialysis, although haemoglobin levels were comparable in the two groups. When haemodialysis patients were stratified by median RBC age (47.7 days), patients with shortened RBC age had higher intradialytic ultrafiltration rates, larger erythropoiesis-stimulating agent doses, and a higher erythropoietin resistance index compared to that of those with preserved RBC age.Conclusions: Haemolysis exists in patients receiving maintenance haemodialysis, especially in those with greater intradialytic fluid extraction.