bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbiological and physicochemical parameters for predicting quality of fat and low‐fat raw ground beef during refrigerated aerobic storage
Authors: Valerio Francesca , Skandamis Panagiotis N. , Failla Sebastiana , Contò Michela , Di Biase Mariaelena , Bavaro Anna Rita , Pirovano Maria Paola , Lavermicocca Paola 
Published: January 2020

AbstractThe aim of the current study was to identify quality indicators of fat (14.50 ± 0.75%) and low‐fat (4.79 ± 0.63%) raw ground beef by monitoring changes in physicochemical and microbiological parameters during aerobic refrigerated storage, such as water‐holding capacity, pH, thiols, carbonyl compounds, thiobarbituric acid reactive substances (TBARS), metmyoglobin, deoxymyoglobin, oxymyoglobin color indices, pseudomonads, Brochothrix thermosphacta, and total viable counts. Meat packaged in air‐permeable polyethylene plastic film was stored under controlled isothermal conditions (0, 5, 10, and 15 °C). A population level of pseudomonads equal to 7.0 ± 0.5 log10 colony forming units (CFU)/g was considered as the potential spoilage level. Using principal component analysis, samples were distinguished on the basis of their microbial load. A significant positive correlation between microbial population and carbonyls, metmyoglobin, TBARS, water‐holding capacity, and a negative correlation with thiols and color parameters (L*, chroma) were observed. Two different approaches were followed to estimate the quality status of samples: (i) the partial least square (PLS) regression with R2 of 0.93 and root mean square error prediction of 0.44 for pseudomonads, using the above physicochemical characteristics as the dominant input variables, which allowed prediction of the microbiological status of ground beef regardless of time–temperature storage profile and fat content, and (ii) a square‐root‐type model (adjusted R2 of 0.952) that satisfactorily predicted the growth of spoilage pseudomonads under isothermal and dynamic conditions, regardless of the above physicochemical changes. The above results suggest that depending on the available input data, the two modeling approaches can accurately (and complementarily) assess quality of aerobically stored ground beef.Practical ApplicationChanges in appearance and quality of fat and low‐fat raw ground beef are associated with physicochemical alteration and/or microbial growth. The study provides two different modeling approaches that can be integrated in an intelligent interface of the refrigerator having specific colorimetric and/or temperature sensors, to evaluate in a convenience way the quality of stored meat thus reducing domestic waste: the partial least square model was based on physicochemical parameters (particularly chroma, metmyoglobin, and thiobarbituric acid reactive substances), while the square root model was based on the time–temperature conditions during storage.
Two‐step biological approach for treatment of rapeseed meal
Authors: Tie Yu , Li Li , Liu Jun , Liu Chaolan , Fu Junjie , Xiao Xiongjun , Wang Guoqiang , Wang Jingfeng 
Published: January 2020

AbstractRapeseed meal (RSM) is an important source of protein, but its value is limited due to the poor digestibility and the presence of many antinutritional factors. In this study, a two‐step biological method was developed for detoxifying RSM and increasing its protein value. In the first stage, various detoxifying enzymes and proteases were produced by Aspergillus niger during solid‐state fermentation (SSF). In the second stage, coordinated enzymatic hydrolysis was employed to further degrade the antinutritional factors and macromolecular proteins in the fermented RSM. Following fermentation at 30 °C for 48 hr and enzymatic hydrolysis at 45 °C for 24 hr, the content of trichloroacetic acid soluble protein (TCA‐SP) and glucosinolates (GLS) in RSM was increased by 81.70% and reduced by 30.06%, respectively, compared with that obtained using the SSF process alone. Moreover, to improve the efficiency of enzymatic hydrolysis, the yield of acid protease was increased by optimizing the composition of the medium so that the TCA‐SP content was increased to 208.39 mg/g and accounted for 51.62% of the total RSM protein, which was 99.36% and 629.66% higher than that in the fermented RSM and control, respectively. Overall, these results demonstrate that the two‐step process could be more effective for the degradation of the antinutritional factors and improvement of the protein quality of RSM compared to use of the SSF method alone, which may improve the utilization of RSM in food and animal feed.Practical ApplicationRapeseed meal (RSM) is a protein source that provides high‐quality nutrition and can be applied to the development of value‐added products for humans and animal feed. To improve the utilization of RSM, a combined method of solid‐state fermentation and enzymatic digestion was developed. Compared with the traditional solid‐state fermentation method, the present method further improves the quality of RSM and demonstrates improved efficacy in increasing the small peptide content while reducing the levels of antinutritional factors.
Dazzling or Fantastically Dull? Re‐Examining the Eighteenth‐Century London Masquerade
Authors: Kobza Meghan 
Published: January 2020

AbstractThis article provides an overview of the social history of the eighteenth‐century London subscription masquerade through a critical analysis of quantitative and qualitative data collected from a cohort study of newspapers in the Burney Collection. An examination of trends in prevalence, financial requirements and use of spatial and social boundaries indicates that the masquerade operated as a space of elite sociability. This new work on the masquerade provides a break from the traditional depiction of the entertainment as carnivalesque cultural trope and challenges the existing scholarship on leisure history and the history of consumer culture during this period.
Comparing control options for time‐series RNA sequencing experiments in nonmodel organisms: An example from grasses
Authors: Qiu Fan , Bachle Seton , Nippert Jesse B. , Ungerer Mark C. 
Published: January 2020

AbstractRNA sequencing (RNA‐seq) is a widely used approach to investigate gene expression and increasingly is used in time‐course studies to characterize transcriptomic changes over time. Two primary options are available as controls in time‐course experiments: samples collected at the first sampling time are used as controls (temporal control, TC) and samples collected in parallel at each individual sampling time are used as controls (biological control, BC). While both approaches are used in experimental studies, we know of no analyses performed to date that directly compare effects of control type choices on identifying differentially expressed genes (DEGs) and subsequent functional analysis. In the current study, we compare experimental results using these different control types for time‐course RNA‐seq drought stress experiments in two wild grass species in the genus Paspalum. Our results showed BC assemblies gave a higher number of loci in both species. The number of DEGs increased with increasing stress and then decreased dramatically at the recovery time point using both control types. Expression levels of the same DEGs were highly correlated between control types in both species, ranging from r = .653 to r = .852. We also observed similar rank orders of shared enriched Gene Ontology term lists using the two different control types. Collectively, our findings suggest similar results in differential gene expression and functional annotation between control types. The ultimate choice of control type will rely on the experimental length and organism type, with labour time and sequencing costs as additional factors to be considered.
Associations of serum citrate levels with knee structural changes and cartilage enzymes in patients with knee osteoarthritis
Authors: Bian Fuqin , Ruan Guangfeng , Xu Jianhua , Wang Kang , Wu Juan , Ren Jiale , Chang Bingru , Ding Changhai 
Published: January 2020

AbstractObjectiveThe aim of this study was to investigate cross‐sectional associations between serum levels of citrate and knee structural changes and cartilage enzymes in patients with knee osteoarthritis (OA).MethodA total of 137 subjects with symptomatic knee OA (mean age 55.0 years, range 34‐74, 84% female) were included. Knee radiography was used to assess knee osteophytes, joint space narrowing (JSN) and radiographic OA assessed by Kellgren‐Lawrence (K‐L) grading system. T2‐weighted fat‐suppressed fast spin echo magnetic resonance imaging (MRI) was used to determine knee cartilage defects, bone marrow lesions (BMLs) and infrapatellar fat pad (IPFP) signal intensity alternations. Colorimetric fluorescence was used to measure the serum levels of citrate. Enzyme‐linked immunosorbent assay was used to measure the serum cartilage enzymes including matrix metalloproteinase (MMP)‐3 and MMP‐13.ResultsAfter adjustment for potential confounders (age, sex, body mass index), serum citrate was negatively associated with knee osteophytes at the femoral site, cartilage defects at medial femoral site, total cartilage defects, and total BMLs (odds ratio [OR] 0.17‐0.30, allP < .05). Meanwhile, serum citrate was negatively associated with IPFP signal intensity alteration (OR 0.30,P = .05) in multivariable analyses. Serum citrate was significantly and negatively associated with MMP‐13 (β −3106.37,P < .05) after adjustment for potential confounders. However, citrate was not significantly associated with MMP‐3 in patients with knee OA.ConclusionSerum citrate was negatively associated with knee structural changes including femoral osteophytes, cartilage defects, and BMLs and also serum MMP‐13 in patients with knee OA, suggesting that low serum citrate may be a potential indicator for advanced knee OA.
A loop‐mediated isothermal amplification assay for the detection of Dahlia mosaic caulimovirus in Dahlia (Dahlia variabilis)
Authors: Iftikhar Romana , Moyo Lindani , Pappu Hanu R. 
Published: January 2020

AbstractDahlia mosaic virus (DMV), an aphid‐transmitted caulimovirus in the family Caulimoviridae, is an important pathogen of dahlia (Dahlia variabilis). Development of rapid, and sensitive diagnostic tools is essential in surveillance and management of DMV. A loop‐mediated isothermal amplification (LAMP) assay was developed for DMV detection. The LAMP assay targets the reverse transcriptase region of the DMV genome and is optimal at a temperature of 60°C and run time of 60 min, and amplification was detected through fluorescence detection and by agarose gel electrophoresis. The assay detected DMV in DNA concentration as low as 10−3 ng. To the best of our knowledge, this is the first report of a LAMP assay for the detection of DMV, a plant DNA virus. This assay will be useful in rapid and sensitive detection of DMV and in reducing the virus incidence through production of virus‐free planting material.
Randomized Controlled Trial of Adult Therapeutic Coloring for the Management of Significant Anxiety in the Emergency Department
Authors: Rajendran Naveendran , Mitra Tatum Priyambada , Shahrestani Sara , Coggins Andrew 
Published: January 2020

AbstractBackgroundAnxiety and acute distress are significant concerns in the emergency department (ED). Adult coloring books are often utilized as an effective means of relaxation in waiting rooms and newsstands, but there are no reported randomized trials examining their effectiveness as a treatment for anxiety.MethodsWe set out to examine the effectiveness of adult coloring books using a randomized placebo‐controlled trial at a university‐affiliated tertiary ED. Anxiety was measured using a validated self‐reporting score, the Hospital Anxiety and Depression Scale (HADS‐A), with a range of 0 to 21. Patients with HADS‐A ≥ 7 were randomly assigned to either an adult coloring pack (n = 26) or placebo pack (n = 27). The primary outcome measure was the within‐patient change in HADS‐A scores following 2 hours of exposure.ResultsA convenience sample of 117 patients were screened, and 53 patients were randomized. Characteristics of allocated groups were similar in terms of sex, diagnosis, and ethnicity. A higher proportion of intervention subjects spent ≥1 hour engaged with their activity (46.2% vs. 4.0%, p = 0.01). For the primary outcome measure, the mean within‐patient decrease in HADS‐A score at 2 hours for intervention subjects was 3.7 (95% confidence interval [CI] = 2.4 to 5.1, p < 0.001) versus a decrease of 0.3 (95% CI = –0.6 to 1.2, p = 0.51) in the placebo group.ConclusionsAmong ED patients, exposure to adult coloring books resulted in lower self‐reported levels of anxiety at 2 hours compared to placebo.
Cost‐effectiveness of icotinib versus whole‐brain irradiation with or without chemotherapy in EGFR‐mutant NSCLC patients with brain metastases
Authors: Li Wenqian , Bai Rilan , Qian Lei , Chen Naifei , Zhao Yuguang , Han Fujun , Bai Ling , Li Jiaxuan , Yu Yu , Cui Jiuwei 
Published: January 2020

AbstractPurposeNonsmall cell lung cancer (NSCLC) patients with brain metastases (BM) have a poor prognosis. Despite the traditional methods including radiotherapy and chemotherapy, epidermal growth factor receptor‐tyrosine kinase inhibitors (EGFR‐TKIs) might benefit patients on survival and quality of life. We investigated the cost‐effectiveness of icotinib compared with whole‐brain irradiation (WBI) with or without chemotherapy for NSCLC patients with BM.Materials and methodsA Markov model was conducted based on the data of BRAIN trial. We compared the economic benefit between icotinib and the combination of WBI and WBI plus chemotherapy group. We considered disease progression as intracranial progression and overall progression separately. Sensitivity analyses were performed to observe the stability of the model. The willingness‐to‐pay (WTP) was set as 3× per capita gross domestic product ($25929/quality‐adjusted life year [QALY]) from the Chinese healthcare perspective.ResultsWhen considering progression as intracranial progression and overall progression, respectively, the incremental cost‐effectiveness ratio was $14 882.64/QALY and $13 484.21/QALY between icotinib and WBI/WBI‐chemotherapy. Besides, both of the average cost‐effective ratio (ACER) and net benefit showed advantage of icotinib (ACER: $34 521.42/QALY for intracranial progression and $36 562.63/QALY for overall progression; net benefit: –$8407.36 for intracranial progression and –$9836.41 for overall progression). One‐way sensitivity analyses demonstrated that no thresholds were encountered. The probabilistic sensitivity analyses showed even at a WTP under $18 000/QALY, icotinib could be cost‐effective.Conclusion: Icotinib was cost‐effective compared with WBI with or without chemotherapy.
Male Breast Cancer in Australia
Authors: Lomma Chris , Chan Arlene , Chih HuiJun , Reid Christopher , Peter Willsher 
Published: January 2020

AbstractAimsMale breast cancer is rare and treatment recommendations are based on female breast cancer guidelines. We analyzed an Australian dataset of patients with early breast cancer (including ductal carcinoma in situ, DCIS) for demographic, pathological and treatment information. The primary objective was to compare treatment delivery for males versus females.MethodsAustralian data from the BreastSurgANZ Quality Audit (BQA) from 1 October 2006 and 30 September 2016 were analyzed. Demographic and pathological information was obtained and compared between males and females. Treatment recommendations were compared to BreastSurgANZ Key Performance Indicators (KPIs) and National Comprehensive Cancer Network (NCCN) guidelines to assess for adherence to national and international guidelines, respectively.ResultsA total of 99,768 breast cancer episodes were analyzed, comprising 585 males (544 invasive; 41 DCIS) and 99 183 (99.4%) females (85 596 invasive; 13 525 DCIS; 62 unknown). Compared with females, males were older at diagnosis, more likely to be hormone receptor‐positive and lymph node‐positive disease, and more likely to have mastectomy. The proportion of males undergoing breast conserving surgery receiving radiotherapy was the only BreastSurgANZ KPI that was not met. Males were less likely to receive adjuvant chemotherapy than females using NCCN guidelines.ConclusionAustralian males with breast cancer account for 0.6% of breast cancer incidence and have similar clinico‐pathological features as reported internationally. Overall, there is good compliance with the surgical KPIs, and adherence to NCCN guidelines for adjuvant systemic treatment is similar to previous international studies.
Cutaneous sebaceous carcinoma
Authors: Wu Albert , Rajak Saul N , Huilgol Shyamala C , James Craig , Selva Dinesh 
Published: January 2020

AbstractCutaneous sebaceous carcinoma occurs almost exclusively on the head and neck and has a significant propensity for recurrence and metastasis. It is easily mistaken for benign conditions, resulting in inappropriate management. Thus, it is important to maintain a high index of suspicion. Despite previous reports, sebaceous carcinoma may occur with similar frequency in Asians and whites. Recent genetic data suggest there are multiple mutational groups of sebaceous carcinoma, paving the way for targeted treatment. After a diagnosis, investigations for staging and for Muir–Torre syndrome should be considered. The available evidence on the treatment options for sebaceous carcinoma is discussed, and specific recommendations for management are made.
Heart disease, hospitalisation and referral: Coaching to Achieving Cardiovascular Health through cardiac rehabilitation in Queensland
Authors: Field Patricia , Franklin Richard C , Barker Ruth , Ring Ian , Leggat Peter , Canuto Karla 
Published: January 2020

AbstractObjectivesTo describe rates of hospitalisation and Coaching on Achieving Cardiovascular Health referral, for Queensland's adults with heart and related disease, and comparisons between Aboriginal and Torres Strait Islander and non‐Indigenous peoples in northern Queensland.DesignDescriptive retrospective epidemiological study of Queensland Health Patient Admission Data Collection for adults with heart and related disease, and Coaching on Achieving Cardiovascular Health referral data. Relative risk and age standardisation were calculated for Aboriginal and Torres Strait Islander and non‐Indigenous peoples.ParticipantsQueensland's adults ≥20 years, hospitalised with heart and related disease (1 January 2012‐31 December 2016).SettingQueensland, Australia.Main outcome measuresQueensland Health Hospital and Health Services’ hospitalisation and Coaching on Achieving Cardiovascular Health referral rates for heart and related disease.ResultsQueensland's Aboriginal and Torres Strait Islander peoples have a higher hospitalisation rate for heart and related disease, with higher rates for northern Queensland. Queensland's overall Coaching on Achieving Cardiovascular Health referral rates were low, but higher for Aboriginal and Torres Strait Islander peoples. Deficiencies in documentation of Aboriginal and Torres Strait Islander people's status affected results in some areas.ConclusionQueensland's Aboriginal and Torres Strait Islander peoples were more likely to be admitted to hospital for heart and related disease and referred to Coaching on Achieving Cardiovascular Health than non‐Indigenous peoples. However, hospitalisation and Coaching on Achieving Cardiovascular Health referral rates are unlikely to reflect the needs of Aboriginal and Torres Strait Islander peoples especially in rural and very remote areas given their higher mortality and morbidity rates and fewer services.
Asclepius and Yellow Ribbon techniques: Efficacious alternative strategies for advancing a coronary sinus electrophysiology catheter
Authors: Chen Tse‐Wei , Huang Mu‐Shiang , Lu Wei‐Da , Wu Yu‐Hao , Chen Ju‐Yi 
Published: January 2020

AbstractBackgroundInserting an electrophysiological (EP) catheter into the coronary sinus (CS) via the femoral vein can be difficult and time‐consuming in patients with variants of the CS orifice or lumen curve. Our experience with such patients inspired us to develop two new techniques: the Asclepius and Yellow Ribbon techniques.MethodsData from a 4‐year period were retrieved from records of patients undergoing radiofrequency ablation for paroxysmal supraventricular tachycardia (PSVT) or Wolff–Parkinson–White (WPW) syndrome. Data were analyzed to determine the success and complication rates of conventional and alternative techniques for catheter placement.ResultsThe success rate of the Asclepius technique was 96.7% (30/31) and that of the Yellow Ribbon technique was 100.0% (7/7). The overall success rate of these two techniques was 97.3% (37/38).ConclusionsWith a high success rate, shorter procedure time, and no complications, the Asclepius and Yellow ribbon techniques may be safe, inexpensive, and effective alternative strategies for EP catheter placement in patients with difficult coronary sinus orifice access.
Short and long course neoadjuvant therapy compared for management of locally advanced rectal cancer: 11 years' experience at a regional centre
Authors: Ngoo Alexander G. , Tan Alexander H. M. , Mushaya Chrispen D. , Ho Yik‐Hong 
Published: January 2020

AbstractBackgroundRectal cancer treatment outcomes for socioeconomically disadvantaged and regional patients have been suggested to be suboptimal in Australia. We investigate outcomes at a regional tertiary centre in order to determine the prognostic impact of patient and treatment factors.MethodsPatients who underwent short and long course neoadjuvant therapy followed by surgery for stage II–III rectal cancer over an 11‐year period were identified. Results were analysed to determine oncological and surgical outcomes along with whether patient and treatment‐related variables were prognostic. Accessibility/Remoteness Index of Australia (ARIA) and Index of Relative Socioeconomic Disadvantage (IRSD) was used to determine remoteness and socioeconomic status, respectively.ResultsA total of 207 patients underwent short (n = 103, 49.8%) and long course (n = 104, 50.2%) over the time period; 81.6% (n = 169) were from outer regional, remote or very remote communities and 55.1% travelled >200 km for treatment; 57.0% were in the most disadvantaged three IRSD deciles. Five‐year cancer‐specific survival, recurrence‐free survival and local recurrence were 83.1% (n = 172), 76.3% (n = 158) and 7.3% (n = 15), respectively. Wound complications were higher in outer regional, remote or very remote patients (25.4% versus 13.2%, P = 0.03). Remoteness, socioeconomic indices, distance to treatment and neoadjuvant type were not prognostic for any other oncological or surgical outcomes on univariate or multivariate analysis.ConclusionsDespite demography suggesting geographic and socioeconomic barriers, oncological and surgical outcomes at our regional centre were comparable to international and Australian trials. Further, these factors were not prognostic. Geographically remote patient's may safely have neoadjuvant modality individualized without compromising care.