bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of STEAP1 in the biological behavior of gastric cancer
Authors: Zhang Zhe , Hou Wen-bin , Zhang Chao , Zhang Dong-dong , An Wen , Pan Si-wei , Wu Wan-di , Chen Qing-chuan , Xu Huimian 
Published: June 2020

Abstract
Background: Six-transmembrane epithelial antigen 1 (STEAP1) is associated with the occurrence and development of cancer. This study aimed to clarify the role of STEAP1 in gastric cancer tumor growth and metastasis, as well as its molecular mechanism of action.Methods: Statistical methods were used for clinical data analysis. Protein expression was detected using immunohistochemistry. The mRNA and protein expression in the cell cultures were detected using reverse transcription-polymerase chain reaction and western blot analysis. Overexpression and silencing models were constructed using plasmid and lentivirus transfection. To detect cell proliferation in vitro, Cell Counting Kit-8, flow cytometry, and colony formation assays were used; transwell and wound healing assays were used to detect cell migration and invasion; RNA sequencing was used for identifying differentially expressed genes; ELISA assay was used to detect the secretory proteins in cells. For in vivo experiments, nude BALB/c mice were used for detecting subcutaneous tumorigenesis and intraperitoneal implantation.Results: STEAP1 was overexpressed in gastric cancer tissues and cell lines. Single factor and Cox analyses showed that STEAP1 gene expression level correlated with poor prognosis. Upregulation of STEAP1 increased cell proliferation, migration, and invasion, which decreased after STEAP1 was knocked down. These changes were achieved via the activation of the AKT/FoxO1 pathway and epithelial-mesenchymal transformation (EMT). The RNA sequencing results indicated that STEAP1 was closely related to inflammatory reactions. STEAP1 can regulate the inflammation-related molecules, IL-1β and IL-6, via the NF-kB and ERK/c-Jun signaling pathways. The in vivo animal experiments showed that STEAP1 knock down, resulted in a decrease in the subcutaneous tumor and peritoneal tumor formation.Conclusion: STEAP1 was overexpressed in gastric cancer and closely associated with OS. STEAP1 can regulate the cell cycle via the Akt/FoxO1 pathway to influence cell proliferation. STEAP1 may affect cell migration and invasion via EMT action. In addition, STEAP1 may mediate the inflammatory response by regulating IL1β and IL6 via the NF-kB and the ERK/c-Jun signaling pathways.
Screening and identification of key microenvironment-related genes in non-functioning pituitary adenoma
Authors: Guo Jing , Wu Jiangping , Fang Qiuyue , Liu Yulou , Li Chuzhong , Zhang Yazhuo 
Published: June 2020

Abstract
Background
Non-functioning pituitary adenoma (NFPA) is a very common type of intracranial tumor, some of which perform locally invasive and high recurrence rate. The tumor microenvironment (TME) shows a high correlation with tumor pathogenesis and prognosis. This study aimed to identify microenvironment-related genes in NFPAs and assess the prognosis value of them.
Methods
73 NFPAs tumor samples were collected from Beijing Tiantan Hospital and obtained transcriptional expression profile by microarray analysis. The immune and stromal scores of each sample were calculated by the ESTIMATE algorithm, and the patients were divided into a high and low immune/stromal scores group. Intersection differentially expressed genes (DEGs) were then obtained to construct a protein-protein interaction (PPI) network. Potential functions and pathways of intersection DEGs were then analyzed by GO (gene ontology) and KEGG (Kyoto Encyclopedia of genes and genomes). Finally, the prognosis value of these genes was evaluated.
Results
The Kaplan-Meier curve indicated that the group of high immune scores were significantly related with poor recurrence-free survival. Then, we identified 497 intersection DEGs (478 over-expressed and 19 under-expressed genes) based on high- vs. low immune/stromal scores groups. Function enrichment analyses of 497 DEGs and hub genes from PPI network showed these genes mainly related with the immune/inflammatory response, T cell activation, and phosphatidylinositol 3 kinase-protein kinaseB (PI3K-Akt) signaling pathway. Among the IDEGs, 23 genes were further verified significantly expressed between invasive group and non-invasive group and 5 genes were highly associated with prognosis of NFPAs.
Conclusion
We screened out a series of critical genes associated with the tumor microenvironment in NFPAs. These genes may play a fundamental role in the development and prognosis of NFPA and may develop new therapeutic targets.
A microcosmic syndrome differentiation model for metabolic syndrome with multi-label learning
Authors: Xia Shujie , Zhang Jia , Du Guodong , Li Shaozi , Yan Zhaoyang , Xin Jiliang , Zhu Long , Gao Bizhen , Li CanDong 
Published: June 2020

Abstract
Background: Metabolic Syndrome (MS) is a complex multi-system disease. Traditional Chinese medicine (TCM) is satisfactory in preventing and treating MS. Syndrome differentiation is the basis of TCM treatment, which is composed of location and/or nature syndrome elements. Many studies have found TCM syndromes are correlated with biological indicators. However, there are various data that mainly come from point-to-point studies, which can’t fully summarize the many to many relationship of TCM syndromes and biological indicators. Thus, it is also hard to deal with the problem that several types of syndromes may possibly happen to a patient at once in the real world. The purpose of this study is to find out the potential relationship between microcosmic index and syndrome elements from a holistic view by means of multi-label learning (MLL) technique, and to provide a multi-label model for TCM syndrome differentiation. Methods: The standardization scale on TCM four diagnostic information for MS is designed, which is used to obtain the results of TCM diagnosis. The model of microcosmic syndrome differentiation is constructed based on 39 physicochemical indexes, such as BMI, abdominal circumference, blood pressure，platelet，fasting blood sugar, insulin, blood lipid，by MLL techniques, called ML-kNN. First, the multi-label learning method is compared with three commonly used single learning algorithms. Then, the comparison of results of ML-kNN between physicochemical indexes and TCM information is also made. Next. the influence of parameter k of ML-kNN to the diagnostic model is investigated and then the best k-value is chosen for the TCM diagnosis. Results: A total of 698 cases are collected for the modeling of the microcosmic diagnosis of MS. The comprehensive performance of the ML-kNN model works obviously better than the others, whose average precision of diagnosis reach 71.4%. The results of ML-kNN based on microcosmic indexes are close to the results based on TCM information. The k value has less influence on the prediction results of ML- kNN. Conclusions: The MLL techniques facilitate building microcosmic syndrome differentiation model in MS and the experiments show this is a practical approach to solve the problem of labeling multiple syndromes simultaneously. Besides, it is also suggested that there is many to many relationships between TCM syndrome elements and physicochemical indexes, which will be conducive to the future objective and comprehensive study of syndrome differentiation in MS.
The Alternations of Endocannabinoid System and its Therapeutic Potential in Autism Spectrum Disorder
Authors: Zou Mingyang , Liu Yu , Xie Shu , Wang Luxi , Li Dexin , Li Ling , Xia Wei , Sun Caihong , Wu Lijie 
Published: June 2020

Abstract
Background: Autism spectrum disorder (ASD) is a group of developmental disabilities whose etiology remains elusive. The endocannabinoid (eCB) system modulated neurotransmission and neuronal plasticity. Evidence points to the involvement of this neuromodulatory system in the pathophysiology of ASD. Herein we investigated if there was disruption of eCB system in ASD and if pharmacological modulators of eCB system might have a therapeutic potential. Methods: We examined three major components of eCB system, namely, endogenous cannabinoids, their receptors and associated enzymes, in ASD children as well as in VPA-induced ASD animal model. Meanwhile we specially increased 2-arachidonylglycerol (2-AG) by administering JZL184, a selective inhibitor of monoacylglycerol lipase, the hydrolytic enzyme for 2-AG, and to examine ASD-like behaviors in VPA-induced rats. Results: Autistic children and VPA-induced rats exhibited the reduced eCBs content, elevated degrading enzymes, and up-regulation of CBRs. We found that repetitive and stereotypical behaviors, hyperactivity, sociability, social preference, and cognitive functioning were improved after acute and chronic JZL184 treatment. The major efficacy of JZL184 was observed at the dose of 3mg/kg for both affecting eCB system and ASD-like behaviors. Conclusions: There were a lower eCB signaling in autistic children and ASD animal model, and boosting 2-AG could ameliorate ASD-like phenotypes in animals. Collectively, the results suggest a novel approach to ASD treatment.
Predicting Pathological Complete Response (Pcr) After Stereotactic Ablative Radiation Therapy (Sabr) of Lung Cancer Using Quantitative Dynamic [18f]fdg Pet and Ct Perfusion: A Prospective Exploratory Clinical Study
Authors: Yang Dae-Myoung , Palma David A. , Kwan Keith , Louie Alexander V. , Malthaner Richard , Fortin Dalilah , Rodrigues George B. , Yaremko Brian P. , Laba Joanna , Gaede Stewart , Warner Andrew , Inculet Richard , Lee Ting-Yim 
Published: June 2020

Abstract
Background:Stereotactic ablative radiation therapy (SABR) is effective in treating inoperable stage I non-small cell lung cancer (NSCLC), but imaging assessment of response after SABR is difficult. This prospective study aimed to develop a predictive model fortrue pathologic complete response (pCR) to SABR using imaging-based biomarkers from dynamic [18F]FDG-PET and CT Perfusion (CTP).Methods:Twenty-six patients with early-stage NSCLC treated with SABR followed by surgical resection were included, as a pre-specified secondary analysis of a larger study. Dynamic [18F]FDG-PET provided maximum and mean standardized uptake value (SUV) and kinetic parameters estimated using a previously developed flow-modified two-tissue compartment model while CTP measured blood flow, blood volume and vessel permeability surface product. Using recursive partitioning analysis (RPA), the imaging-based biomarkers for predicting pCRwere assessedand compared to current RECIST (Response Evaluation Criteria in Solid Tumours version 1.1) and PERCIST (PET Response Criteria in Solid Tumours version 1.0) criteria.Results: RPA identified threepatient groups based on tumour blood volume before SABR (BVpre-SABR) and change in SUV­max (ΔSUVmax).The highest true pCR rate of 92%was observed in the group withBVpre-SABR< 9.3 mL/100g and ΔSUVmax< -48.9% after SABR while the worst was observed in the group with BVpre-SABR ≥ 9.3 (0%). A logistic regression model based on RPA risk groupsshowedexcellentpCRprediction(Concordance: 0.92; P=0.03). RECIST and PERCIST showed poor pCRprediction (Concordance: 0.54 and 0.58, respectively).Conclusions: In this study, we developed a predictive model based on dynamic [18F]FDG-PET and CT Perfusion imaging that was significantly better than RECIST and PERCIST criteria to predict pCR of NSCLC to SABR. This model warrants validation with larger sample size studies.Trial registration:MISSILE-NSCLC, NCT02136355 (ClinicalTrials.gov). Registered May 8, 2014, https://clinicaltrials.gov/ct2/show/NCT02136355
Longitudinal Changes in Sports Activity From Pre-diagnosis to First Five Years Post-diagnosis: a Prospective Chinese Breast Cancer Cohort Study
Authors: Lei Yuanyuan , Ho Suzanne C. , Kwok Carol , Cheng Ashley , Cheung Ka Li , Lee Roselle , Yeo Winnie 
Published: June 2020

Abstract
Background: To compare change in level of physical activitybetween pre-and post- diagnosis of breast cancer in Chinese women.Methods:Based on an on-going prospective study consisting of 1462 Chinese women with early-stage breast cancer, a validated modified Chinese Baecke questionnaire was used to measure physical activity at baseline (12 months before cancer diagnosis), 18-, 36- and 60-months after diagnosis (over the previous 12 months before each interview). Results:The overall physical activity level at post-diagnosis was 5.8 MET-hours/week, which was significantly higher than that at pre-diagnosis at a median level of 0.6 MET-hours/week (P <0.001).The median levels of physical activity at 18-, 36- and 60-months follow-up were5.3, 4.4 and 3.9 MET-hours/week, respectively. There was no significant difference between any two of the three follow-ups at post-diagnosis. The proportions of participant who met WCRF/AICR recommendation before and after cancer diagnosis were both low, being 20.7% and 35.1%, respectively.Compared to pre-diagnosis, most of the patients improved or had no change on level of physical activity at post-diagnosis, with the respective proportion being 48.2% and 43.8%. Conclusions:Adherence to current lifestyle recommendation for cancer survivors, Chinese women with breast cancer significantly increasedlevel of physical activity level after cancer diagnosis, and such improvement was sustained to five years post-diagnosis. The proportion of patients who met the exercise recommendation for cancer survivors was still low. Encouraging patients on the importance of durable high level of physical activity in breast cancer survivorship is warranted.
Ethnobotanical Knowledge on Non-conventional Food and Medicinal Plants in Rio Cajari Extractivist Reserve, Amazon, Brazil
Authors: Filho Galdino Xavier de Paula , Ribeiro Adivair Freitas , Moraes Alcidete Flexa , Penha Willis Freitas , Borges Wardsson Lustrino , Santos Ricardo Henrique Silva 
Published: June 2020

Abstract
Background:Information on the knowledge, management and ways of using food and medicinal plants by traditional populations, family farmers and Brazilian native population in the Amazon is essential to guarantee the sovereignty of these groups. The objective of this study was to evaluate the diversity, knowledge and ways of using non-conventional food and medicinal plants in traditional communities in a conservation unit in the Brazilian Amazon.Methods:This study was conducted using semi-structured interviews applied to local respondents. Fifty-six residents were interviewed in 26 communities. The Indices of Use Value (UVI) and relative frequency of species citation (Fr) were evaluated; also, their diversity and equitability using the Shannon- Wiener (H’) Pielou (J’) indices, respectively. The species were listed according to their family, scientific name, popular names, categories of use, propagation environment, growth habit, medicinal indications, domestication status, production cycle and herbarium registration.Results:A total of 269 species of both non-conventional food and medicinal plants were identified, distributed in 84 botanical families, 198 genera, in addition to 13 unidentified species. The Arecaceae and Lamiaceae families had the highest species richness (11 and 7, respectively). Eryngium foetidum L. (Apiaceae) and Ipomoea potatoes L. (Convolvulaceae) presented the highest relative citation frequencies (19.7 and 19.3, respectively) and the highest index of use value of the species (0.94 and 0.92, respectively). The Shannon-Wiener (H’) and Pielou (J’) diversity indices were considered high (5.02 and 0.9, respectively) when compared to other ethnobotanical works carried out in Brazil and in the Amazon.Conclusions:A wide relationship of use was observed between the species under study and the population of this conservation area. In the environment in which these families are found, of geographical isolation and distance from urban centers, these species become, in many circumstances, the only food and medicinal resources, therefore, being fundamental to the sovereignty of these families.
The effectiveness of implementation strategies for promoting evidence informed interventions in allied healthcare: A Systematic Review
Authors: Goorts Kaat , Dizon Janine , Milanese Steve 
Published: June 2020

Abstract
Background- Evidence based practice in health care has become increasingly popular over the last decades. Many guidelines have been developed to improve evidence informed decision making in health care organisations, however it is often overlooked that the actual implementation strategies for these guidelines are as important as the guidelines themselves. The effectiveness of these strategies is rarely ever tested specifically for the allied health therapy group.Objectives - To explore the evidence for implementation strategies within allied health. This review sought to explore the effectiveness of implementation strategies for promoting evidence informed interventions in allied healthcare.Data sources- Cochrane, Medline, Embase and Scopus were searched from 2000 onwards.Study eligibility : Level I and II studies were included if an evidence informed implementation strategy was tested in allied health personnel.Study appraisal and synthesis methods: The SIGN method was used to evaluate risk of bias. The National Health and Medical Research Council (NHMRC) model was applied to evaluate the grade for recommendation.Results- A total of 490 unique articles were identified, with 6 primary studies meeting the inclusion criteria. Three different implementation strategies and three multi-faceted components strategies were described. We found moderate evidence for educational meetings, local opinion leaders and patient mediated interventions. We found stronger evidence for multi-faceted components strategies.Conclusion- Few studies describe the effectiveness of implementation strategies for allied healthcare, but evidence was found for multi-faceted components for implementing research in an allied health therapy group population.
Rainwater harvesting and primary uses among rural communities in Ghana
Authors: Owusu Seth , Asante Rachel 
Published: June 2020

AbstractRainwater harvesting (RWH) has been practised over many decades for various purposes, including domestic use. The practice contributes largely to sustainable development in terms of providing ‘free’ water to many people. This study assessed the primary uses and benefits of rainwater to rural residents by conducting a questionnaire survey of 48 households actively practising RWH. Samples of the rainwater were also tested in the laboratory to examine the quality and potential health risks. The results show that rainwater was used for potable and non-potable purposes by the locals particularly in the rainy season. Bathing accounted for the most use (53%), while the potable use was just 7%. The majority of users (73%) reported a positive impact of the practice on their lives, and the reliability of rainwater was highly ranked. The physicochemical properties of rainwater were good, but the study highlights the need for better management of the technology to ensure optimal quality. Overall, the findings provide substantial evidence on the benefits of harvesting rainwater and recommend greater use of rainwater, especially in areas with no or limited access to a public water supply.
PDA-cross-linked beta-cyclodextrin: a novel adsorbent for the removal of BPA and cationic dyes
Authors: Wang Jianyu , Cheng Guang , Lu Jian , Chen Huafeng , Zhou Yanbo 
Published: June 2020

Abstract
In this study, 4,4′-(hexafluoroisopropene) diphthalic acid (PDA)-CD polymers containing β-cyclodextrin (CD) were synthesized for the adsorption of endocrine disrupting chemicals (EDCs) and dyes. It features great adsorption of bisphenol A (BPA), methylene blue (MB) and neutral red (NR). The maximum adsorption capacities of MB, NR and BPA can reach 113.06, 106.8 and 51.74 mg/g, respectively. The tandem adsorption results revealed that adsorptions of dyes and BPA onto PDA-CD polymer were two independent processes: non-polar BPA entrapment by cyclodextrin cavities while dyes were captured by the carboxyl groups and π-π stacking interactions. The adsorption processes performed well in a wide range of pH (4.0–10.0) and were not affected by fulvic acid (FA) and inorganic ions.
The effects of offshore petroleum exploitation on microbial community and antibiotic resistome of adjacent marine sediments
Authors: Wang Jing , Zhou Jiti 
Published: June 2020

Abstract
The exploitation of petroleum in offshore areas is becoming more prosperous due to the increasing human demand for oil. However, the effects of offshore petroleum exploitation on the microbial community in the surrounding environment are still not adequately understood. In the present study, variations in the composition, function, and antibiotic resistance of the microbial community in marine sediments adjacent to an offshore petroleum exploitation platform were analyzed by a metagenomics-based method. Significant shifts in the microbial community composition were observed in sediments impacted by offshore petroleum exploitation. Nitrosopumilales was enriched in marine sediments with the activities of offshore petroleum exploitation compared to the control sediments. The abundances of function genes involved in carbon, butanoate, methane, and fatty acid metabolism in sediment microbial communities also increased due to the offshore petroleum exploitation. Offshore petroleum exploitation resulted in the propagation of some antibiotic resistance genes (ARGs), including a multidrug transporter, smeE, and arnA, in marine sediments via horizontal gene transfer mediated by class I integrons. However, the total abundance and diversity of ARGs in marine sediments were not significantly affected by offshore petroleum exploitation. This study is the first attempt to analyze the impact of offshore petroleum exploitation on the spread of antibiotic resistance.
Enhanced removal of hexavalent chromium by different acid-modified biochar derived from corn straw: behavior and mechanism
Authors: Xu Yonggang , Bai Tianxia , Yan Yubo , Zhao Yunfeng , Yuan Ling , Pan Peng , Jiang Zhen 
Published: June 2020

Abstract
It is of great significance to remove Cr(VI) from water as a result of its high toxicity. Biochar from corn straw was modified by different acids (HNO3, H2SO4 and H3PO4) to remove Cr(VI) from aqueous solution. To estimate the removal mechanisms of Cr(VI) by the acid-modified biochars, batch experiments were performed in the light of contact time, Cr(VI) concentration, and pH, and the characteristics of acid-modified biochars before and after Cr(VI) adsorption were investigated by Fourier transform infrared spectra (FTIR) and X-ray photoelectron spectroscopy (XPS). The adsorption kinetics of Cr(VI) by acid-modified biochars were consistent with the pseudo-second-order model, and the adsorption isotherm obeyed the Freundlich model. Furthermore, the acid- modified biochars could supply more oxygen-containing functional groups (-COOH and -OH) as electron donor (e−) and hydrogen ion (H+) to enhance the reduction of Cr(VI) to Cr(III), resulting in enhanced removal of Cr(VI). HNO3-modified biochar exhibited the highest removal efficiency of Cr(VI). In general, the acid modifition of biochar was an effective method to increase the removal of Cr(VI).