bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Characterization and Antibiotic Resistance of Acinetobacter baumannii in Cerebrospinal Fluid and Blood
Authors: Shi Xiaohong , Wang Hong , Wang Xin , Jing Huaiqi , Duan Ran , Qin Shuai , Lv Dongyue , Fan Yufeng , Huang Zhenzhou , Stirling B. Kyle , Zhang Lei , Wang Jiazheng 
Published: January 2020

Abstract
BackgroundThe increasing rates of carbapenem-resistant Acinetobacter baumannii (CRAB) caused nosocomial infections generate significant comorbidity and sometime cause death among patients. Current treatment options are limited. These infections pose great difficulties for infection control and clinical treatment. This study identifies the antimicrobial resistance, carbapenemases and genetic relatedness of A. baumannii isolates from cerebrospinal fluid (CSF) and blood in a hospital in Shandong, China. Methods A total of 50 nonrepetitive CSF A. baumannii isolates and 44 blood isolates were collected. The resistance phenotypes were determined according to the Clinical and Laboratory Standards Institute guidelines. We performed Polymerase Chain Reaction (PCR) experiments to detect the carbapenem resistance mechanism. Finally, we conducted Multilocus Sequence Typing (MLST) to depict the genetic relatedness of these isolates. Results We observed that eighty-eight of the 94 isolates collected were resistant to imipenem or meropenem. Among them, the bla OXA-23 gene was the most prevalent carbapenemase gene with a 91.5% (86/94) detection rate, followed by the bla OXA-24 gene that showed a 2.1% (2/94) detection rate isolates. Among all CRAB observations in this study, isolates with the bla OXA-23 gene were resistant to both imipenem and meropenem. However, isolates positive for the bla OXA-24 gene but negative for the bla OXA-23 gene showed an imipenem-sensitive but meropenem-resistant phenotype. The outcome of multilocus sequence typing analysis showed 21 different STs were distinguished, of which ST195 (25.5%), ST540 (12.8%) and ST208 (11.7%) were most frequently observed. Eighty of the 94 isolates (85.1%) were clustered into CC92, and all CC92 isolates showed a carbapenem resistance phenotype (except AB13). Five novel STs were detected, and most of them were CRAB, some of which belonged to CC92. Conclusion A high level of carbapenem resistance was detected in this study. The CC92 and bla OXA-23 gene were predominant. Five novel STs were detected, and these new STs require further investigation to understand the nature of and to prevent outbreaks caused by A. baumannii . Our study provides additional observations and epidemiological data of CSF and blood A. baumannii strains, which may improve future infection control measures and aid in potential clinical treatment in hospitals and other clinical settings.
Nucleated red blood cell and its specific fluctuations are risk factors of 28-day and 90-day all-cause mortality in ICU patients: an observational cohort study
Authors: Wang Jing , Yuan Yuan , Jin Xiaxia , Shen Bo , Lu Guoguang 
Published: January 2020

Abstract
Background Little was known about the first occurrence time of Nucleated red blood cell (NRBC)and the persistent duration of the positive results in ICU patients, nor the dynamic changes of NRBC count and quantified predictive value of NRBC for mortality in ICU patients. We hypothesized that the persistent presence of NRBC in ICU patients would be associated with all-cause mortality and death patients would have specific NRBC fluctuations compared to survival ones.Methods A total of 224 newly admitted ICU patients in our hospital were collected and were followed up to 90 days after admission to ICU. Biological information, clinical characteristics and laboratory indicators and dynamic changes of NRBC count was compared between survival group and death group. Factors plausibly interact with both NRBC and outcome were included in logistic regression analysis to explore the risk factors of 28-day and 90-day mortality,ROC curve were drawn to determine the predictive value of NRBC for 28-day and 90-day all-cause mortality for ICU patients.Results NRBC was positively correlated with NRBC-positive duration days, APACHE II score, SOFA score, CCI, CRP, PCT and RDW. The 28-day mortality and 90-day were 15.0%,31.3%, 41.2%, 56.7% and 32.7%,52.9%, 59.4%, 80.0% in patients with 0/μL, 1–100/μL, 101–200/μL and more than 200/μL NRBC,respectively.NRBC in the death group had a rapid upward trend before death, while those in the survival group were stable at low levels.NRBC was a robust predictor of 28-day and 90-day all-cause mortality following multivariable adjustment. The adjusted odds of 28-day and 90-day all-cause mortality in patients with more than 200/μL NRBC were 5.087(95% CI, 1.960-13.202) and 4.922(95% CI, 1.369-17.703), relative to patients without NRBC. Area under the curve(AUC) of predicting 28-day all-cause mortality and 90-day all-cause mortality by NRBC were 0.685 and 0.670, and NRBC had high predictive values for sepsis patients and non-respiratory failure patients.Conclusion In ICU patients especially sepsis patients and non-respiratory failure patients, the presence of NRBC is a robust predictor of 28-day all-cause mortality and 90-day all-cause mortality. Specific NRBC fluctuations were found in death patients compared to survival ones.
Identification of differentially expressed circulating exosomal lncRNAs in IgA nephropathy patients
Authors: Yang Qiongqiong , Guo Na , Zhou Qin , Huang Xiang , Yu Jianwen , Han Qianqian , Nong Baoting , Xiong Yuanyan , Liang Peifeng , Li Jiajia , Feng Min , Lv Jun 
Published: January 2020

Abstract
Background: Immunoglobulin A nephropathy (IgAN) is one of the most prevalent primary glomerular disease. Non-invasive biomarkers are urgently needed for IgAN diagnosis. We investigate the difference in expression profiles of exosomal long non-coding-RNAs (lncRNAs) in plasma from IgAN patients compared with their healthy first-degree relatives, which may reveal novel non-invasive IgAN biomarkers. Methods: Exosomes were isolated from the plasma of IgAN patients and their healthy first-degree relatives. High-throughput RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) was used to validate lncRNA expression profiles. Pathway enrichment analysis was used to predict their nearest protein-coding genes. Results: lncRNA-G21551 was significantly down-regulated in IgAN patients. The nearest protein-coding gene was found to be encoding the low affinity receptor of the Fc segment of immunoglobulin G (FCGR3B). Conclusion: Exosomal lncRNA-G21551, with FCGR3B as the nearest protein-coding gene, was down-regulated in IgAN patients, indicating its potential to serve as a non-invasive biomarker for IgAN.
Halogens in amphibole and mica from mantle xenoliths: Implications for the halogen distribution and halogen budget of the metasomatized continental lithosphere
Authors: Hecker Johannes G. , Marks Michael A.W. , Wenzel Thomas , Markl Gregor 
Published: January 2020

Abstract
This study reports halogen contents (F and Cl) of amphibole and phlogopite derived from mantle xenoliths and one peridotite massif, for amphibole and phlogopite megacrysts and ultramafic magmatic cumulates (hornblendites) found in alkaline volcanic rocks from 12 localities in Europe and Africa. Amphibole and phlogopite contain more F than Cl with F/Cl ratios reaching about 160 in phlogopites and 50 in amphiboles. Phlogopites are higher in F (median of 3400 μg/g) than amphibole (median of 1000 μg/g), while median Cl contents are higher in amphibole (290 μg/g) compared to phlogopite (180 μg/g).
The Cl contents and the F/Cl ratios in amphibole and phlogopite from mantle xenoliths exhibit large differences between samples of the same region, recording very large variations of halogen contents in the continental lithosphere. We suggest that the halogen content in such samples largely depends on the initial composition of percolating melts and fluids in the continental lithosphere. During reaction of these agents with peridotitic wall-rocks, Cl is preferentially retained in the fluid as it is much more incompatible compared to water and F. This desiccation effect continuously increases salinity (Cl content) and decreases the F/Cl ratio in the agent with time, causing variable Cl contents and F/Cl ratios in amphibole and phlogopite at a specific locality. Subsequent partial melting processes may then sequester and re-distribute, especially Cl among amphibole, phlogopite and melts/fluids as a result of its strong incompatibility, whereas F is much less affected as it behaves slightly compatible. The impact of even small amounts of amphibole and mica on the total halogen budget in the continental lithosphere is significant and both minerals can effectively contribute to the high halogen contents typical of alkaline melts.
Política educacional no ensino superior: reflexões sobre a contribuição da assistência estudantil da UFRPE
Authors: Macedo Juliana Cavalcanti , Abranches Ana de Fátima Pereira de Sousa 
Published: January 2020

Esta pesquisa teve como principal objetivo analisar a política de assistência estudantil da UFRPE (Sede), a partir da percepção dos estudantes beneficiados. A investigação se baseou no método quali-quanti. Dessa forma, foram aplicados questionários e realizadas entrevistas com os estudantes assistidos. O estudo revelou que a maioria dos estudantes avalia como boa a política de assistência estudantil da UFRPE, assim como, afirma que a assistência estudantil contribui muito para a sua permanência na universidade. Contudo, também revela algumas situações que precisam ser reavaliadas e melhoradas.
Analysis of Routine and Integrative Data from Clostridioides difficile Infection Diagnosis and the Consequent Observations
Authors: Piatti Gabriella , Bruzzone Marco , Fontana Vincenzo , Ceppi Marcello 
Published: January 2020

Background:Clostridioides difficileInfection (CDI) is an acute disease that needs a fast proper treatment. Unfortunately, the diagnosis, and above all the understanding of the results, remain arduous.Objective:This study analyzed routine and integrative results of all fecal samples from patients over time. Our aim was to understand the dynamics of CDI infection and the meaning of “difficult to interpret” results, to make physicians better understand the various tools they can use.Methods:We evaluated routine results obtained from 815 diarrheal stools with Enzyme Immunoassay (EIA) that detectsC. difficileGlutamate Dehydrogenase (GDH) antigen and toxin B. We also reanalyzed a part of samples using integrative tests: a Real-time polymerase chain reaction (RT-PCR) forC. difficiletoxin B gene (tcdB) and the automated immunoassay VIDASC. difficilesystem for GDH and toxins A/B.Results:EIA GDH positivity increased through multiple testing over time, with aPvalue <0.001, depicting a sort of bacterial growth curve. Eighty-five percent of GDH positive/toxin B negative,i.e., discrepant, samples PCR weretcdBpositive, 61.5% of discrepanttcdBpositive samples were VIDAS toxins A/B positive, and 44.4% of GDH EIA negative stools were VIDAS GDH positive.Conclusion:The results confirmed the low sensitivity of the EIA system forC. difficileGDH and toxins, questioned the use of the latter for concluding any CDI diagnostic algorithm, and led us to indicate the algorithm beginning with tcdB molecular research, and continuing in positive cases with VIDAS CD GDH method, as the most effective for CDI.
Clinical Evaluation of Patients with a Delayed Treatment of Anterior Cruciate Ligament Rupture
Authors: Acosta-Olivo Carlos , Tamez-Mata Yadira , González-Robles Jaime , Dávila-Martínez Agustín , Vilchez-Cavazos Félix , Peña-Martínez Victor , de la Garza-Castro Santiago , Villarreal-Villarreal Gregorio 
Published: January 2020

Background:
Isolated ACL lesions can occur in up to 44.5% of sports patients and its association with a meniscal injury can be 30-80%.

Objective:
The aim of our study was to evaluate and compare clinical function of the knee in patients with reconstruction of the ACL, with or without meniscal injury.

Methods:
This was a retrospective study during a four-year period of patients with ACL repaired injury. Inclusion criteria were indistinct gender, >18 years of age with a primary ACL repaired injury (with or without associated meniscal injury). The exclusion criterion were an associated knee injury (except meniscal injury), an associated fracture in the lower limb, previous knee surgery, reconstruction surgery, graft failure after 7 months, rheumatological or psychiatric disease. The Tegner Lysholm Knee Scoring Scale, the International Knee Documentation Committee (IKDC) form and a Visual Analog Scale (VAS) were applied. The patients were divided into groups, ≤1 year and >1 year of follow-up after surgery, and in ACL injury alone or ACL plus meniscal injury.

Results:
A total of 126 ACL injuries were analyzed. No significant difference was observed between groups in demographic data. In the patients with meniscal injury, the medial meniscus was involved in 24 (50%) cases, and the lateral meniscus 22 (46%). No difference was observed between groups in the evaluation with the Lysholm-Tegner score, IKDC and VAS.

Conclusion:
Patients with isolated ACL lesions or ACL lesions plus meniscal injuries, treated with partial meniscectomy, presented a similar clinical and functional evolution even after four years of treatment.
The Spectrum of Microbial Keratitis: An Updated Review
Authors: Bartimote Christopher , Foster John , Watson Stephanie 
Published: January 2020

Background:In microbial keratitis, infection of the cornea can threaten vision through permanent corneal scarring and even perforation resulting in the loss of the eye. A literature review was conducted by Karsten, Watson and Foster (2012) to determine the spectrum of microbial keratitis. Since this publication, there have been over 2600 articles published investigating the causative pathogens of microbial keratitis.Objective:To determine the current spectrum of possible pathogens implicated in microbial keratitis relative to the 2012 study.Methods:An exhaustive literature review was conducted of all the peer-reviewed articles reporting on microbial pathogens implicated in keratitis. Databases including MEDLINE, EMBASE, Scopus and Web of Science were searched utilising their entire year limits (1950-2019).Results:Six-hundred and eighty-eight species representing 271 genera from 145 families were implicated in microbial keratitis. Fungal pathogens, though less frequent than bacteria, demonstrated the greatest diversity with 393 species from 169 genera that were found to cause microbial keratitis. There were 254 species of bacteria from 82 genera, 27 species of amoeba from 11 genera, and 14 species of virus from 9 genera, which were also identified as pathogens of microbial keratitis.Conclusion:The spectrum of pathogens implicated in microbial keratitis is extremely diverse. Bacteria were most commonly encountered and in comparison, to the review published in 2012, further 456 pathogens have been identified as causative pathogens of microbial keratitis. Therefore, the current review provides an important update on the potential spectrum of microbes, to assist clinicians in the diagnosis and treatment of microbial keratitis.
Hubungan komposisi tubuh dengan kesegaran jasmani pada atlet hockey
Authors: Latifah Nandita Nury , Margawati Ani , Rahadiyanti Ayu 
Published: January 2020

Komposisi tubuh yang tidak optimal dapat menurunkan kesegaran jasmani atlet sehingga dapat terjadi penurunan performa ketika bertanding. Tujuan dari penelitian ini adalah mengetahui hubungan persen lemak tubuh, persen massa otot, dan somatotype dengan kesegaran jasmani pada atlet hockey. Desain penelitian yaitu cross-sectional dengan jumlah subjek 35 atlet di Pelatihan Hockey Universitas Negeri Semarang dan Universitas Negeri Yogyakarta yang dipilih dengan purposive sampling. Data persen lemak tubuh dan persen massa otot diukur menggunakan bioelectrical impedence analysis (BIA). Data somatotype menggunakan metode antropometri Heath-Carter dan data kesegaran jasmani berupa nilai VO2max diambil menggunakan metode Balke. Analisis data menggunakan uji Pearson, Rank Spearman dan uji regresi linier. Sebagian besar atlet hockey (54,3%) memiliki nilai VO2max dengan kategori baik. Nilai persen lemak tubuh terendah 6,8% dan nilai tertinggi 35%. Nilai persen massa otot terendah 23,9% dan nilai tertinggi 40,9%. Nilai rata-rata dari nilai endomorph, mesomorph, dan ectomorph adalah (-0,04)±0,3; 4,2±0,9; dan 2,3±1,2. Ada hubungan yang signifikan antara persen lemak tubuh (p<0,001), persen massa otot (p<0,001), nilai endomorph (p<0,001), dan nilai ectomorph (p=0,016) dengan nilai VO2max. Variabel yang paling berkaitan dengan nilai VO2max adalah persen lemak (p<0,001). Correlation of body composition with physical fitness of hockey athletes AbstractSuboptimal body composition could reduce the physical fitness and performance of athletes. This study analyzed the relationship of percent body fat, percent muscle mass and somatotype on physical fitness in hockey athletes. This study was a cross-sectional study with 35 athletes in Pelatihan Hockey Universitas Negeri Semarang and Universitas Negeri Yogyakarta which selected by purposive sampling. Percent body fat and percent muscle mass were measured using bioelectrical impedance analysis (BIA). Somatotype measurement used the anthropometric Heath-Carter method and physical fitness (VO2max) measurement by the Balke method. Data were analyzed using the Pearson test, Rank-Spearman test, and Linear Regression test. Most hockey athletes (54.3%) had good VO2max value. The lowest percentage of body fat was 6.8%. The highest percentage of muscle mass was 40.9%. The average value of the endomorph, mesomorph, and ectomorph values were (-0.04) ± 0.3; 4.2 ± 0,9; and 2.3 ± 1.2. There were a significant relationship between percent body fat (p <0.001), percent muscle mass (p <0.001), endomorph value (p <0.001), and ectomorph value (p = 0.016) with VO2max value. Percent of body fat was the most related variable to VO2max (p<0.001).
PRILAKU PENYELESAIAN PERSAMAAN LOTKA-VOLTERRA AKIBAT WAKTU TUNDA (TIME DELAY )
Authors: La Gubu La Gubu 
Published: January 2020

Model prey-predator Lotka-Volterra dengan waktu tunda merupakan model interaksi satu prey dan satu predator. Model ini melibatkan persamaan integral dan sistem persamaan diferensial non linear autonomous. Sistem persamaan tersebut dapat dilinearkan dengan menggunakan Transformasi Laplace disekitar titik kesetimbangannya, kemudian ditentukan prilaku penyelesaiannya dengan menggunakan hampiran solusi. Sistem persamaan ini mempunyai dua titik kesetimbangan yaitu titik (0,0) dan titik 1122 ,a a . Untuk memeriksa apakah titik kesetimbangan tersebut stabil atau tidak dapat dilihat dari nilai-nilai eigen yang diperoleh. Hasil analisis menunjukkan bahwa titik 1122 ,a a merupakan titik kesetimbangan yang stabil asimtotik.
PERANCANGAN SISTEM KRIPTOGRAFI KURVA ELIPTIK PADA AUDIO DIGITAL TERKOMPRESI
Authors: Kasim Anita Ahmad 
Published: January 2020

Penelitian ini bertujuan melakukan perancangan sisten kriptografi kurva eliptik pada data audio digital terkompresi sehingga diperoleh sebuah sistem kriptografi yang dapat melakukan proses enkripsi dan dekripsi berkas audio digital terkompresi. Data audio digital yang asli wav dan data audio terkompresi .mp3 akan dienkripsi menghasilkan berkas audio terenkripsi. Pengujian sistem dilakukan dengan memproses berkas pesan audio dalam sistem menggunakan parameter kurva eliptik. Parameter dan nilai field p akan diubah-ubah untuk mengetahui pengaruh sistem kriptografi kurva eliptik terhadap berkas audio terkompresi. Panjang berkas audio pun akan dibuat bervariasi, untuk melihat waktu yang dibutuhkan sistem untuk mengenkripsi berkas dengan panjang berkas yang berbeda-beda. Pengujian akan dilakukan terhadap 5 buah berkas terkompresi .mp3 yang berasal dari berkas .wav. Masing-masing berkas akan dienkripsi untuk melihat perubahan yang terjadi pada berkas ketika dienkripsi dalam sistem. Hasil yang diperoleh dalam penelitian ini aalah sebuah system yang mampu melakukan proses enkripsi dan dekripsi audio menggunakan proses kriptorafi kurva eliptik.
OPTIMALISASI PRODUKSI ROTI DENGAN MENGGUNAKAN METODE GOAL PROGRAMMING (STUDI KASUS : UKM IBARAKI BAKERY KOTA PALU)
Authors: Balomba A , Sahari A , Jaya A I 
Published: January 2020

OPTIMALISASI PRODUKSI ROTI DENGAN MENGGUNAKAN METODE GOAL PROGRAMMING (STUDI KASUS : UKM IBARAKI BAKERY KOTA PALU)
PELABELAN GRACEFUL GANJIL PADA GRAF DUPLIKASI DAN SPLIT BINTANG
Authors: Bantara J A , Sudarsana I W , Musdalifah S 
Published: January 2020

Graph is not an empty a finite of the objects that called point (vertex) with the couple was not that is the side (edge). The set point denoted by , while the set edge denoted by . Odd graceful labeling on graph with side is a function injective from so that induced function such that in label with so label sides would be different. A graph that have an odd graceful labeling is called odd graceful graph. The result showed that duplicate star graph for and split star graph for , for even satisfie odd graceful labeling.
PERUBAHAN DISTRIBUSI MERKURI (Hg) TERHADAP WAKTU DI SEDIMEN SUNGAI POBOYA
Authors: Febrianti I , Ratianingsih R , Puspita J W 
Published: January 2020

Poboya is illegal gold mining area at Palu City. The amalgamate process of gold extraction is prepared traditionally using mercury. Tailing of this process which contains mercury is throwed away to the ground. The mercury contain will infiltrate to the soil water and later on pollute Poboya’s river. Related to the mercury that categorized as dangerous material, this research purposes to investigate the mercury distribution changing at Poboya’s river sediment. The mercury distribution changing is investigated by modify the advection-diffusion equation model. The model was completed by the initial conditions and Neumann boundary conditions. To get the numerical solutions, it is used a numerical scheme namely Duffort Frankel finite difference method for the second derivative, and Center Scheme for the first derivative. The solution represents the mercury distribution changing with respect to time at the Poboya’s river sediment. The simulation result explains that 0,0521 ppm mercury is distributed from the upper bound (current source) observation domain following the sediment direction (to estuary) caused by the advection process and decreased due to the diffusion process. For , the mecury was distributed 0,00285 m to the estuary direction with the mercury concentration is 0,005 ppm, until , mercury was distributed 0,00832 m to estuary with mercury concentration is 0,005 ppm. In fact that at the estuary (lower bound), the 0,0244 ppm mercury that was already deposited will be diffused in an opposite direction. The advection process and the low initial mercury concentration, makes the reached distribution distance is no longer far comparing to the opposited mercury distribution. For the mercury was distributed 0,000822 m to the upper direction with mercury concentration is 0,005 ppm, until , the mercury was distributed 0,000873 m with mercury concentration is 0,005 ppm