bluuebunny/crossref_metadata_2025_split huggingface dataset:
The De Winter‐like electrocardiogram pattern in inferior and lateral leads associated with left circumflex coronary artery occlusion
Authors: Chen Qingxing , Zou Tian , Pang Yang , Ling Yunlong , Zhu Wenqing 
Published: December 2020

AbstractThe aim of this report was to investigate coronary artery occlusion related to De Winter‐like electrocardiogram (ECG) pattern. A patient admitted to Zhongshan Hospital who was diagnosed with acute coronary syndrome with De Winter‐like ECG pattern was reviewed in our case report. In this case, we found that a De Winter‐like ECG change in the inferior and lateral leads was associated with left circumflex coronary artery occlusion. After a timely reperfusion therapy, the symptom was obviously alleviated with a normalization of upsloping ST‐segment. Doctors should be aware that De Winter‐like ECG changes can also predict acute occlusion in any coronary artery, not only in the left anterior descending artery and right coronary artery but also in the left circumflex coronary artery.
On the N‐Arylation of Acetamide Using 2‐, 3‐ and 1’‐Substituted Iodoferrocenes**
Authors: Kadari Lingaswamy , Erb William , Halauko Yury S. , Ivashkevich Oleg A. , Matulis Vadim E. , Lyakhov Dmitry , Roisnel Thierry , Radha Krishna Palakodety , Mongin Florence 
Published: December 2020

AbstractVarious 2‐, 3‐ and 1’‐substituted iodoferrocenes were reacted with acetamide in the presence of copper(I) iodide (1 equiv), N,N’‐dimethylethylenediamine (1 equiv), tripotassium phosphate (2 equiv) in dioxane at 90 °C for 14 h, and allowed a large range of original 1,2‐, 1,3‐ and 1,1’‐disubstituted ferrocenes to be obtained. The results were compared as a function of the substituent and its position on the ring. DFT calculations revealed higher activation barrier for the oxidative addition in the ferrocene series when compared with classical planar aromatics. Structure‐property relationships were applied to rationalize the reactivity of the different iodoferrocenes.
Reactions of a 3‐Phenyl‐1‐trifluoromethyl‐prop‐2‐yne Iminium Salt with Furans, Thiophenes, and Pyrroles
Authors: Schneider Thomas , Heinrich Georg , Koch Raphael , Maas Gerhard 
Published: December 2020

AbstractReactions of a novel propyne iminium salt, N,N‐dimethyl 3‐phenyl‐1‐trifluoromethyl‐propyne iminium triflate, with electron‐rich heteroaromatic ring systems (furans, thiophenes, pyrroles) are reported. The 1‐CF3‐propyne iminium ion can act as a highly reactive ambident electrophile, giving rise to simple electrophilic heteroaromatic ring substitution products, and as a 1,3‐biselectrophile leading to C2+C3‐CF3 annulation products. Moreover, it is an electron‐deficient alkyne which was found to afford double [2+2] cycloaddition products with 2,5‐dimethylfuran and 2,5‐dimethylthiophene. The obtained molecular structures include CF3‐containing compounds with cyclopenta[b]furan, cyclopenta[b]thiophene, cyclopenta[c]thiophene, cyclopenta[b]pyrrole, cyclopenta[c]pyrrole, 3H‐pyrrolizine, and 1,4‐dihydrocyclopenta[b]pyrrole ring systems. Analogous reactions of 1,3(or 1,4)‐phenylenebis(1‐CF3‐propyne iminium) salts with furans, pyrroles or thiophenes in a 2 : 1 stoichiometry lead to phenylene‐tethered condensed heterocycles of the same type.
Harnessing the power of electrophoresis and chromatography: Offline coupling of reverse phase liquid chromatography‐capillary zone electrophoresis‐tandem mass spectrometry for analysis of host cell proteins in monoclonal antibody producing CHO cell line
Authors: Kumar Ramesh , Shah Rohan L. , Ahmad Shadab , Rathore Anurag S. 
Published: December 2020

AbstractHost cell proteins (HCPs) are widely regarded as a critical quality attribute for a biotherapeutic product. Bottom up MS is the present gold standard for HCP analysis but suffers from incomplete protein identification due to complex nature of the HCP mixture and limited separation efficiency of the preceding LC‐based systems. In this paper, we present for the first time an application involving use of LC‐CE‐MS/MS platform for analysis of HCPs. It has been demonstrated that the proposed platform has been able to successfully identify 397 HCPs from the supernatants of recombinant Chinese hamster ovary cells, twice and thrice the number of proteins identified by the state‐of‐the‐art LC‐MS/MS (189 HCPs) and CE‐MS/MS (128 HCPs) analyses, respectively. Of these, 225 HCPs were unique to the LC‐CE‐MS/MS approach and were not identified by either LC‐MS/MS or CE‐MS/MS. It is observed that the LC‐CE‐MS/MS platform combines the benefits of LC‐MS/MS and CE‐MS/MS techniques and identifies peptides in a wider range of size, pI, and hydrophobicity. Additionally, LC‐CE‐MS/MS also identified more HCPs associated with cellular components, molecular functions, biological processes, peptidases, and secretory proteins. The proposed approach would thus be a useful addition in HCP analysis and secretome studies of mAb‐producing Chinese hamster ovary cells.
New insights on the ventral attention network: Active suppression and involuntary recruitment during a bimodal task
Authors: Solís‐Vivanco Rodolfo , Jensen Ole , Bonnefond Mathilde 
Published: December 2020

AbstractDetection of unexpected, yet relevant events is essential in daily life. fMRI studies have revealed the involvement of the ventral attention network (VAN), including the temporo‐parietal junction (TPJ), in such process. In this MEG study with 34 participants (17 women), we used a bimodal (visual/auditory) attention task to determine the neuronal dynamics associated with suppression of the activity of the VAN during top‐down attention and its recruitment when information from the unattended sensory modality is involuntarily integrated. We observed an anticipatory power increase of alpha/beta oscillations (12–20 Hz, previously associated with functional inhibition) in the VAN following a cue indicating the modality to attend. Stronger VAN power increases were associated with better task performance, suggesting that the VAN suppression prevents shifting attention to distractors. Moreover, the TPJ was synchronized with the frontal eye field in that frequency band, indicating that the dorsal attention network (DAN) might participate in such suppression. Furthermore, we found a 12–20 Hz power decrease and enhanced synchronization, in both the VAN and DAN, when information between sensory modalities was congruent, suggesting an involvement of these networks when attention is involuntarily enhanced due to multisensory integration. Our results show that effective multimodal attentional allocation includes the modulation of the VAN and DAN through upper‐alpha/beta oscillations. Altogether these results indicate that the suppressing role of alpha/beta oscillations might operate beyond sensory regions.
Trilaciclib prior to chemotherapy and atezolizumab in patients with newly diagnosed extensive‐stage small cell lung cancer: A multicentre, randomised, double‐blind, placebo‐controlled Phase II trial
Authors: Daniel Davey , Kuchava Vladimer , Bondarenko Igor , Ivashchuk Oleksandr , Reddy Sreekanth , Jaal Jana , Kudaba Iveta , Hart Lowell , Matitashvili Amiran , Pritchett Yili , Morris Shannon R. , Sorrentino Jessica A. , Antal Joyce M. , Goldschmidt Jerome 
Published: December 2020

AbstractTrilaciclib is an intravenous CDK4/6 inhibitor administered prior to chemotherapy to preserve haematopoietic stem and progenitor cells and immune system function from chemotherapy‐induced damage (myelopreservation). The effects of administering trilaciclib prior to carboplatin, etoposide and atezolizumab (E/P/A) were evaluated in a randomised, double‐blind, placebo‐controlled Phase II study in patients with newly diagnosed extensive‐stage small cell lung cancer (ES‐SCLC) (NCT03041311). The primary endpoints were duration of severe neutropenia (SN; defined as absolute neutrophil count <0.5 × 109 cells per L) in Cycle 1 and occurrence of SN during the treatment period. Other endpoints were prespecified to assess the effects of trilaciclib on additional measures of myelopreservation, patient‐reported outcomes, antitumour efficacy and safety. Fifty‐two patients received trilaciclib prior to E/P/A and 53 patients received placebo. Compared to placebo, administration of trilaciclib resulted in statistically significant decreases in the mean duration of SN in Cycle 1 (0 vs 4 days; P < .0001) and occurrence of SN (1.9% vs 49.1%; P < .0001), with additional improvements in red blood cell and platelet measures and health‐related quality of life (HRQoL). Trilaciclib was well tolerated, with fewer grade ≥3 adverse events compared with placebo, primarily due to less high‐grade haematological toxicity. Antitumour efficacy outcomes were comparable. Administration of trilaciclib vs placebo generated more newly expanded peripheral T‐cell clones (P = .019), with significantly greater expansion among patients with an antitumour response to E/P/A (P = .002). Compared with placebo, trilaciclib administered prior to E/P/A improved patients' experience of receiving treatment for ES‐SCLC, as shown by reduced myelosuppression, and improved HRQoL and safety profiles.
Maternal vitamin D, oxidative stress, and pre‐eclampsia
Authors: Wei Shu Qin , Bilodeau Jean‐François , Julien Pierre , Luo Zhong‐Cheng , Abenhaim Haim A. , Bi Wei Guang , Nuyt Anne Monique , Leduc Line , Audibert François , Fraser William D. 
Published: December 2020

AbstractObjectiveTo examine the associations between risk of pre‐eclampsia and pregnancy levels of maternal 25‐hydroxyvitamin D (25[OH]D) and oxidative stress biomarkers.MethodsA nested case–control study (n = 99; 34 cases; 65 controls) within a prospective pregnancy cohort. Maternal 25(OH)D and oxidative stress markers (six isomers of F2‐isoprostanes; F2‐isoPs) were measured in plasma at 12–18 and 24–26 gestational weeks. Vitamin D deficiency was defined as 25[OH]D less than 50 nmol/L.ResultsMaternal vitamin D deficiency was associated with increased 8‐iso‐PGF2α (P = 0.037), 15(R)‐PGF2α (P = 0.004), (±)5‐iPF2α‐VI (P = 0.026) at 12–18 weeks. Vitamin D deficiency was inversely associated with 8‐iso‐PGF2α (P = 0.019) and (±)5‐iPF2α‐VI isomer (P = 0.010) at 24–26 weeks. Both maternal vitamin D deficiency (adjusted odds ratio [aOR], 4.79; 95% confidence interval [CI], 1.67–13.75) and increased (±)5‐iPF2α‐VI (aOR, 2.46; 95% CI, 1.16–5.22) at 24–26 weeks were associated with risk of pre‐eclampsia. However, the interaction test between 25(OH)D and (±)5‐iPF2α‐VI was not significant (P = 0.143).ConclusionPlasma 25(OH)D below 50 nmol/L was associated with increased oxidative stress levels during pregnancy as measured by two F2‐isoP isomers, including the well‐studied marker 8‐iso‐PGF2α. Whether vitamin D‐induced oxidative stress mediates the risk of pre‐eclampsia warrants future study.
[6]‐Gingerol impedes 7,12‐dimethylbenz(a)anthracene‐induced inflammation and cell proliferation‐associated hamster buccal pouch carcinogenesis through modulating Nrf2 signaling events
Authors: Sun Yugang , Ren Jinmin , Wang Fang 
Published: December 2020

AbstractThe present study examines the chemopreventive role of [6]‐gingerol, an active component of ginger, on 7,12‐dimethylbenz[a]anthracene (DMBA)‐induced hamster buccal pouch (HBP) carcinogenesis models. The HBP has been developed with an addition of 0.5% of DMBA to the HBP area three times per week, up to the end of the 16th experimental week. At the end of the experiment, we noticed 100% tumor incidence and precancerous lesions, such as dysplasia, hyperplasia, keratosis, and well‐differentiated squamous cell carcinoma, in DMBA‐induced HBP. Furthermore, we observed that [6]‐gingerol inhibited the increased thiobarbituric acid‐reactive substances and decreased antioxidant levels in DMBA‐induced hamsters. Moreover, [6]‐gingerol inhibits DMBA‐exposed over expression of inflammatory markers (inducible nitric oxide synthase, interleukin [IL]‐1β, IL‐6, cyclooxygenase‐2, and tumor necrosis factor‐α) and cell proliferation markers (cyclin D1, proliferating cell nuclear antigen); induces proapoptotic markers in HBP. Nuclear factor erythroid‐2‐related factor‐2 (Nrf2) is a major antioxidant transcription factor, which regulates the antioxidant gene‐dependent scavenge of tumor proliferation and apoptosis. Overexpression of Nrf2 signaling plays a pivotal role and can be a novel target in preventing carcinogenesis. In this study, [6]‐gingerol restores the DMBA‐induced depletion of Nrf2 signaling and thereby prevents buccal pouch carcinogenesis in hamsters. These results point out that [6]‐gingerol impedes the responses of inflammatory and cell proliferation‐associated progression of cancer through the action of Nrf2 signaling.
Comparative insight into the genomic landscape of SARS‐CoV‐2 and identification of mutations associated with the origin of infection and diversity
Authors: Mishra Divya , Suri Gurparsad Singh , Kaur Gurleen , Tiwari Manish 
Published: December 2020

AbstractThe analyses of 2325 severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) genomes revealed 107, 162, and 65 nucleotide substitutions in the coding region of SARS‐CoV‐2 from the three continents America, Europe, and Asia, respectively. Of these nucleotide substitutions 58, 94, and 37 were nonsynonymous types mostly present in the Nsp2, Nsp3, Spike, and ORF9. A continent‐specific phylogram analyses clustered the SARS‐CoV‐2 in the different group based on the frequency of nucleotide substitutions. Detailed analyses about the continent‐specific amino acid changes and their effectiveness by SNAP2 software was investigated. We found 11 common nonsynonymous mutations; among them, two novel effective mutations were identified in ORF9 (S194L and S202N). Intriguingly, ORF9 encodes nucleocapsid phosphoprotein possessing many effective mutations across continents and could be a potential candidate after the spike protein for studying the role of mutation in viral assembly and pathogenesis. Among the two forms of certain frequent mutation, one form is more prevalent in Europe continents (Nsp12:L314, Nsp13:P504, Nsp13:Y541, Spike:G614, and ORF8:L84) while other forms are more prevalent in American (Nsp12:P314, Nsp13:L504, Nsp13:C541, Spike:D614, and ORF8:L84) and Asian continents (Spike:D614), indicating the spatial and temporal dynamics of SARS‐CoV‐2. We identified highly conserved 38 regions and among these regions, 11 siRNAs were predicted on stringent criteria that can be used to suppress the expression of viral genes and the corresponding reduction of human viral infections. The present investigation provides information on different mutations and will pave the way for differentiating strains based on virulence and their use in the development of better antiviral therapy.
Enhanced growth and yield of oyster mushroom by growth‐promoting bacteria Glutamicibacter arilaitensis MRC119
Authors: Kumari Simpal , Naraian Ram 
Published: December 2020

AbstractPromotion of mushroom growth by means of biological agents replacing chemicals is an emerging and highly demanded issue in the sector of mushroom cropping. The present study was aimed to search for a novel bacterium potentially able to enhance mushroom growth and yield. A total of 2165 bacterial isolates purified from different samples were scrutinized through various growth‐promoting attributes. As a consequence of rigorous screening, 26 isolates found exhibiting positive traits of mushroom growth promotion. Thereafter, in response to the cocultivation (fungus and bacteria), a potent bacterial strain was isolated capable to improve significantly the mycelial growth. In cocultivation the highest radial and linear growth rate was 7.6 and 8.1 mm/day on 10th and 11th days, respectively. The fruitbody yields and biological efficiency (BE) of the inoculated sets were 28% and 58% higher than the uninoculated control sets. The bacterium was molecularly identified based on 16S ribosomal RNA sequencing and confirmed as Glutamicibacter arilaitensis MRC119. Therefore, the bioinoculant of the current bacterium can be potentially useful as an ecofriendly substitute stimulating the production of mushroom fruit bodies with improved BE.
The combined ratio of estrogen, progesterone, Ki‐67, and P53 to predict the recurrence of endometrial cancer
Authors: Jia Mingzhu , Jiang Peng , Huang Zhen , Hu Jing , Deng Ying , Hu Zhuoying 
Published: December 2020

AbstractBackground and ObjectivesWe aimed to explore the capacity of the combined ratio of biomarkers to predict the recurrence of Stage I–III endometrial cancer (EC).MethodsA total of 473 patients were enrolled after screening. The cut‐off value of the ratio was calculated by the receiver operating characteristic curve (ROC). The univariate and multivariate Cox regression analysis was used to assess the correlation between the combined ratio and the recurrence of EC. The differences of clinicopathological parameters between the two groups divided based on the threshold were compared.ResultThe ROC curve showed that 0.92 was the optimal cut‐off value of the ratio ([ER + PR]/[P53 + Ki67]). The multivariate analysis demonstrated that only International Federation of Gynecology and Obstetrics stage (p = .031) and the combined ratio (p = .004) were independent risk factors of recurrence. The 3‐year recurrence‐free survival (RFS) and overall survival of patients in the low‐ratio group were 54.1% and 66.8%, respectively; while in the high‐ratio group were 94.9% and 97.9%, respectively (p < .001). The 3‐year RFS of 194 patients, who did not receive the adjuvant therapy, was 54.7% and 97.2% between two groups (p < .001).ConclusionsThe optimal cut‐off value (0.92) of the combined ratio was demonstrated to be better to predict the recurrence of EC than a single immunohistochemical marker.
Seasonal patterns in greenhouse gas emissions from thermokarst lakes in Central Yakutia (Eastern Siberia)
Authors: Hughes‐Allen Lara , Bouchard Frédéric , Laurion Isabelle , Séjourné Antoine , Marlin Christelle , Hatté Christine , Costard François , Fedorov Alexander , Desyatkin Alexey 
Published: December 2020

AbstractIn the ice‐rich permafrost area of Central Yakutia (Eastern Siberia, Russia), climate warming and other natural and anthropogenic disturbances have caused permafrost degradation and soil subsidence, resulting in the formation of numerous thermokarst (thaw) lakes. These lakes are hotspots of greenhouse gas emissions, but with substantial spatial and temporal heterogeneity across the Arctic. We measured dissolved CO2 and CH4 concentrations in thermokarst lakes of Central Yakutia and their seasonal patterns over a yearly cycle. Lakes formed over the Holocene (alas lakes) are compared to lakes that developed over the last decades. The results show striking differences in dissolved greenhouse gases (up to two orders of magnitude) between lake types and seasons. Shallow lakes located in hydrologically closed alas depressions acted as CO2 sinks and strong sources of diffusive CH4 during some seasons (ebullition was not assessed). Recent thermokarst lakes were moderate to extremely high sources of diffusive CO2 and CH4, with considerable accumulation of greenhouse gas under the ice cover (winter) or in the deepest water layers (summer), highlighting the need to include spring and autumn as critical periods for integrated assessments. The water column was stratified in winter (all lake types) and especially in summer (recent thermokarst lakes), generating anoxia in bottom waters and favoring CH4 production and storage, particularly in the most organic‐rich lakes. The diffusive fluxes measured from thermokarst lakes of this typical taiga alas landscape of Central Yakutia are among the highest presented across Arctic and subarctic regions.
Industrialization of hybrid Si/III–V and translucent planar micro‐tracking modules
Authors: Nardin Gaël , Domínguez César , Aguilar Álvaro Fernando , Anglade Laetitia , Duchemin Mathilde , Schuppisser David , Gerlich Florian , Ackermann Mathieu , Coulot Laurent , Cuénod Blaise , Petri Delphine , Niquille Xavier , Badel Nicolas , Lachowicz Agata , Despeisse Matthieu , Levrat Jacques , Ballif Christophe , Askins Stephen , Núñez Rubén , Jost Norman , Vallerotto Guido , Antón Ignacio 
Published: December 2020

AbstractA tracking‐integrated hybrid micro‐concentrator module is presented that can harvest direct, diffuse, and albedo irradiance components. It uses biconvex 180× lens arrays to concentrate direct light on high‐efficiency III–V solar cells (29% module efficiency has been demonstrated outdoors on direct sunlight at Concentrator Standard Test Conditions) and a planar micro‐tracking mechanism to allow installation in static frames. Two architectures have been developed to harvest diffuse irradiance: (1) a hybrid architecture where the backplane is covered with monofacial or bifacial Si cells; (2) a translucent architecture where diffuse light is transmitted through the module for dual‐land‐use applications, such as agrivoltaics. Simulations show that the hybrid architecture provides an excess of yearly energy production compared to 20% efficiency flat‐plate photovoltaic (PV) module in all locations studied, including those with a low direct normal irradiance (DNI) content, and up to 38% advantage in high‐DNI locations. The use of bifacial heterojunction and interdigitated back‐contact Si cells has been explored for the glass–Si–glass backplane laminate to harvest albedo light. Bifacial gains modeled can boost energy yield by about 30% in the best scenario. We discuss the perspectives of the translucent modules for dual‐land‐use applications as well, such as integration in greenhouses for agriculture‐integrated PV (agrivoltaics). This architecture can provide up to 47% excess electricity compared to a spaced reference Si array that transmits the same amount of solar photosynthetically active radiation for crop production. The HIPERION consortium funded by the European H2020 program is making an intensive effort to take this technology to the industrial scale.
Retention and job satisfaction of school psychologists
Authors: Young Ellie L. , Moulton Sara E. , Julian Alexander , Smith Alivia , Butler Rachel 
Published: December 2020

AbstractRetention of current school psychologists is a vital strategy for addressing shortages in the field. Understanding what contributes to why school psychologists stay in the field has the potential to influence leaders and administrators to ensure that they are targeting what matters most to school psychologists. Survey results from 134 school psychologists indicated that they stay in the field because they enjoy working with children and believe their work makes a difference. These generally satisfied school psychologists also shared that they work with teams that facilitate effective and creative problem solving and were acknowledged for their contributions. For the few school psychologists who expressed dissatisfaction, their responses communicated a lack of professional self‐efficacy: their opinions were not valued, they did not believe they were effective in their roles, administrators did not value their contributions, and they did not believe they could make a difference for students. Implications for practice highlight the need for district and building administrators to ensure that school psychologists have manageable caseloads that allow them to work directly with children and to be a part of teams that solve problems.