bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biosynthetic circ-PLEKHA5 stabilized by SRSF7 promotes the vasculogenic mimicry of glioblastoma cells by encoding a novel protein 622aa
Authors: Su Rui , Liu Xiaobai , Teng Hao , Ruan Xuelei , Wang Di , Yang Chunqing , Shao Lianqi , Liu Yunhui , Cai Heng , Li Zhen , Yu Bo , Xue Yixue 
Published: November 2020

Abstract
Background: Increasing studies have been demonstrated that circRNAs play vital regulatory roles in the biological behaviors of glioblastoma cells, and increasing circRNAs are found to have the capacity of encoding small peptides, which are involved in the regulatory process.Methods: Western blot and qRT-PCR were conducted to confirm the expression of SRSF7 and circ-PLEKHA5 respectively. RNase R digestion assay and fluorescence in situ hybridization assays were conducted to evaluate the existence and cellular location of circ-PLEKHA5. RIP assay was used to access the relationship between SRSF7 and circ-PLEKHA5. Dual-luciferase assay and FLAG tag assays were performed to test the coding capability of circ-PLEKHA5. Immunofluorescence assay was conducted to evaluate the location of circ-PLEKHA5-622aa. CCK-8, vasculogenic mimicry (VM) formation and transwell assays were used to evaluate the roles of SRSF7, circ-PLEKHA5, circ-PLEKHA5-622aa on proliferation, VM formation, migration and invasion. Nude mice xenograft studys with PAS-CD34 staining were used to clarify the functional roles of SRSF7 and circ-PLEKHA5 on VM formation in vivo.Results: SRSF7 was up-regulated in glioma, and promoted the proliferation, migration, invasion, VM formation and the expression of VM-associated proteins of glioma cells by increasing the expression of circ-PLEKHA5. Circ-PLEKHA5 was mainly localized in cytoplasm and promoted the proliferation, migration, invasion, VM formation and the expression of VM-associated proteins of glioma cells by encoding a novel protein circ-PLEKHA5-622aa. The application of SRSF7 and circ-PLEKHA5 inhibitor significantly suppressed the tumor growth and VM formation in vivo.Conclusions: This study first demonstrated the coding ability of circ-PLEKHA5, and identified the regulatory roles of SRSF7/circ-PLEKHA5/circ-PLEKHA5-622aa pathway in VM formation of glioblastoma cells. Our findings might provide a novel strategy for glioma treatment.
Intake, Rumen Fermentation and Apparent Digestibility of Aerbasi Cashmere Goats Fed Corn Stover, Caragana and Their Mixture on the Mongolian Plateau
Authors: Wang Wei , Zhou Tianrong , Jia Yushan , Risu Na , Cai Yimin , Gentu Ge 
Published: November 2020

Abstract
To effectively use local available feed resources to address the problem of animal feed shortage in winter, the intake, rumen fermentation and apparent digestibility of Aerbasi cashmere goats fed corn stover (CS), caragana (CA) and their mixture were studied on Mongolian plateau.The treatments were designed as CS, CA, CS 80% + CA 20% (MT1), CS 50% + CA 50% (MT2), and CS 20% + CA 80% (MT3). With an increased mixing ratio of CA, intake of DM, NDF, and half cellulose was decreased (P<0.05). Meanwhile intake of CP, ADF, and acid detergent lignin (ADL) was increased (P<0.05). Compared to the CS treatment, the proportion of particles ≤ 0.1 mm in size was significantly (P<0.05) higher in the CA treatment. With an increased mixing ratio of CA, the proportions of 0.5–1.0 and 1.0–5.0 mm particles decreased (P<0.05), but the proportion of > 5.0 mm particles did not. the proportions of ≤ 0.1 and 0.1–0.5 mm particles increased (P<0.05).The results confirm the mix ration of 4 to 1 has a good mix ratio for goat feed, based on the DM intake, apparent digestibility and VFA contents, and proportion of particles > 1.0 mm in size.
Causes of Hospitalisation Over 2011-2018 Among a Cohort of People With HIV&nbsp;From a London Centre
Authors: Rein Sophia M , Lampe Fiona C , Chaloner Clinton , Stafford Adam , Rodger Alison J , Johnson Margaret A , McDonnell Jeffrey , Burns Fiona , Madge Sara , Miners Alec , Sherr Lorraine , Collins Simon , Speakman Andrew , Phillips Andrew N , Smith Colette 
Published: November 2020

Abstract
Objective: To describe the spectrum of ICD-10 classified causes for hospitalisations occurring between 2011/2012 and 2018 in a cohort of PLHIV.Methods: This sub-study includes 798 HIV-positive individuals participating in the Antiretroviral, Sexual Transmission Risk and Attitudes (ASTRA) questionnaire study who were recruited from a large London centre. A medical record review identified the occurrence and causes of hospitalisation from the date of questionnaire completion (2011-2012) until 1 June 2018. Up to five causes were classified by an HIV clinician using the ICD-10 system.Results: There were 274 hospitalisations in 153 people (rate=5.8/100 person-years; 95% CI: 5.1, 6.5). Causes were wide-ranging; the most common were circulatory (16.8%), digestive (13.1%), respiratory (11.7%), infectious diseases (11.0%), injury/poisoning (10.6%), genitourinary diseases (9.9%) and neoplasms (9.1%). A tenth (27/274) of hospitalisations were related to at least one AIDS-defining illness. Median duration of hospitalisation was 5 days. At the time of hospitalisation, median CD4 count was high (510 cells/µl; IQR: 315-739), while median CD4 nadir was relatively low (113 cells/µl; IQR: 40-239). At admission, half of individuals (51%) had a previous AIDS-defining illness and 21% had viral load >50 copies/ml. Individuals admitted for infectious diseases were particularly likely to have unfavourable clinical characteristics (low CD4, viral non-suppression, not on ART, previous AIDS).Conclusions: In the modern cART era, the spectrum of causes of hospitalisation in PLHIV in the UK is wide-ranging, highlighting the importance of holistic care for PLHIV, including prevention, early detection and treatment of comorbidities.
Unusual Aldehyde Reductase Activity for Production of Full-length Fatty Alcohol by Cyanobacterial Aldehyde Deformylating Oxygenase&nbsp;&nbsp;
Authors: Buttranon Supacha , Intasian Pattarawan , Treesukkasem Nidar , Jaroensuk Juthamas , Maenpuen Somchart , Sucharitakul Jeerus , Lawan Narin , Chaiyen Pimchai , Wongnate Thanyaporn 
Published: November 2020

Abstract
Background: Aldehyde-deformylating oxygenase (ADO) is a non-heme di-iron enzyme that catalyzes deformylation of aldehydes to generate alkanes/alkenes. In this study, we report for the first time that under anaerobic or limited oxygen conditions, Prochlorococcus marinus (PmADO) can generate full-length fatty alcohols from fatty aldehydes without eliminating a carbon unit. Results: Unlike the native activity of ADO which requires electrons from the Fd/FNR electron transfer complex, the aldehyde reduction activity of ADO requires only NADPH. Our results demonstrated that yield of alcohol products can be affected by oxygen concentration and type of aldehyde. Under O2-scant conditions (10-15%), yields of octanol and dodecanol were around 40-60% and could be increased up to 80% under strict anaerobic conditions (>0.0004%). Unexpectedly, Fe2+ cofactor is not involved in the aldehyde reductase activity of PmADO because yields of alcohols obtained from holo- and apo-enzymes were similar under anaerobic conditions. The direct hydride transfer activity of PmADO is highly specific to substrates; NADPH not NADH can be used as a reductant to reduce medium-chain fatty aldehydes (C6-C10) with decanal as the most preferred substrate (the highest kcat/Km value with 98% bioconversion yield). Molecular dynamics (MD) simulations was used to identify a binding site of NADPH which is located close to the aldehyde binding site. In the metabolic engineered cells containing PmADO, dual activities of alkane and alcohol production could be detected. Conclusion: The findings reported herein highlight a new activity of PmADO which may be applied as a biocatalyst for industrial synthesis of fatty alcohols in the future.
Conditional genetic deletion of CSF1 receptor in microglia ameliorates the physiopathology of Alzheimer’s disease
Authors: Pons Vincent , Lévesque Pascal , Plande Marie-Michèle , Rivest Serge 
Published: November 2020

Abstract
BackgroundAlzheimer’s disease (AD) is a progressive neurodegenerative disorder and the most common form of dementia in the world. Microglia are the innate immune cells of CNS, their proliferation, activation and survival in pathologic and healthy brain have previously been shown to be highly dependent on CSF1R.MethodsHere we investigate the impact of such receptor on AD etiology and microglia. We deleted CSF1R using Cre/Lox system, the knock-out (KO) is restricted to microglia in the APP/PS1 mouse model. We induced the knock-out at 3-month-old, before plaque formation and evaluated both 6 and 8-month-old groups of mice.ResultsOur findings demonstrated that CSF1R KO did not impair microglial survival and proliferation at 6 and 8 months of age in APP cKO compared to their littermate controls groups APPSwe/PS1. We have also shown that cognitive decline is delayed in CSF1R-deleted mice. Ameliorations of AD etiology is associated with a decrease in plaque volume in cortex and hippocampus area. A compensating system seems to take place following the knock-out, since TREM2/β-Catenin and IL-34 expression are significantly increased. Such a compensatory mechanism may promote microglial survival and phagocytosis of Aβ in the brain.ConclusionsOur results provide new insights on the role of CSF1R in microglia and how it interacts with the TREM2/β-Catenin and IL-34 system to clear Aβ and ameliorates the physiopathology of AD.
Efficient Method for Isolation of High-Quality RNA from Psidium Guajava L. Tissues
Authors: Carpinetti Paola Avelar , Fioresi Vinicius Sartori , Cruz Thais Ignez , Almeida Francine Alves Nogueira , Canal Drielli , Ferreira Adésio , Ferreira Marcia Flores da Silva 
Published: November 2020

Abstract
Background: Acquiring high-quality RNA in sufficient amounts is necessary in plant molecular biology and genetic studies. Several methods of RNA extraction from plants are available in the literature, mainly due to the great biochemical diversity present in each species and tissue, which can complicate or prevent the extraction. Psidium guajava (Myrtaceae family) is a perennial fruit tree of medicinal and economic value; nevertheless, only few molecular and omics studies are available for the species. One reason for this fact is the difficulty in obtaining the RNA due to the content of the samples, which are rich in polyphenols, polysaccharides and secondary metabolites. Furthermore, there is still no tested or standardized method available for the isolation of RNA from guava or Psidium samples, which hampers advances in the genus.Results: Here we compare the quality and yields of RNA isolated using six extraction protocols: two based on the application of cetyltrimethylammonium bromide (CTAB) lysis buffer, two commercial kits (PureLink™ RNA Mini Kit and RNeasy® Plant Mini Kit), one using the TRIzol® reagent, and one applying guanidine thiocyanate lysis buffer followed by organic phase extraction. RNA integrity, quality and yields were assessed by agarose gel electrophoresis and spectrophotometry. The CTAB-based method provided the highest RNA yields and quality for five different tissues (flower bud, immature leaf, young leaf, mature leaf and root), genotypes and stress conditions. For the most efficient protocol, the average yield of RNA from guava leaf was 210.4 μg/g of tissue, and the A260/A280 and A260/A230 ratios were 2.1 and 2.2, respectively. RT-PCR analysis demonstrated that the purity of the samples was sufficient for molecular biology experiments.Conclusion: CTAB-based methods for RNA isolation were found to be the most efficient, providing the highest RNA yields and quality for tissues from P. guajava. Additionally, they demonstrated to be compatible for downstream RNA-based applications, besides showing the advantages of lower cost and time investments.
Implementation and Sustainability of an Enhanced Pulmonary Rehabilitation Program in a Single Centre
Authors: Braak Kim van der , Wald Joshua , Tansey Catherine , Paes Thais , Sedeno Maria , Selzler Anne-Marie , Stickland Michael , Bourbeau Jean , Janaudis-Ferreira Tania 
Published: November 2020

Abstract
Background: Pulmonary rehabilitation (PR) has major benefits for patients with chronic obstructive pulmonary disease (COPD). The availability of PR in Canada is low. To facilitate implementation, a standardized and enhanced PR Program has been developed with a self-management education intervention that has been validated and shown to be effective. The objectives of our study were to assess the implementation of this program into a single site; determine the sustainability 18 months after implementation; and to identify the satisfaction with, facilitators of and barriers to implementation and sustainability of the program as perceived by patients with COPD and HCPs. Methods: We conducted a prospective pre-post study which consisted of two phases: 1) the implementation phase (first six months after implementation) and 2) the sustainability phase (18 months after implementation). Guided by the RE-AIM framework, outcomes including: Reach (number of patients enrolled and demographics), Effectiveness (Change in patient outcomes), Adoption (HCPs’ characteristics), Implementation (Program fidelity) and Maintenance (Patient outcomes at 3-month follow-up). Results: Reach: Twenty-six patients were included for both phases (two different samples). Effectiveness: Clinically important improvements in patient outcomes were found for functional exercise capacity, knowledge, functional status and self-efficacy in both phases of the study. Adoption: All HCPs involved in PR (N=8) agreed to participate and used the program in both phases of the study. Implementation: Fidelity for the group education sessions ranged from 76-95% in the implementation phase and from 82-88% in the sustainability phase. Maintenance (implementation phase): Clinical important improvements at 3-month follow-up were found in knowledge, self-efficacy for exercise and walking. Patients and HCPs were highly satisfied with the program. Lack of time was reported as the most common barrier and having longstanding procedures as the most common facilitator by HCPs to implementation and sustainability of the program. Conclusions: The enhanced PR program was accepted by the patients and HCPs and could be reliably implemented and maintained at a single expert center. The program provided clinical benefits for patients in terms of functional capacity, knowledge and self-efficacy for walking. These findings will guide planning for wide scale dissemination and implementation of the program.
Olive Extract Ameliorates Oxidative Stress and Inflammation, and Protects Intestinal Villus and Microbiota in Piglets Induced by Lipopolysaccharides
Authors: Zhang Yu , Deng Zhao-Xi , He Mao-Long , Luo Xin M. , Liu Jian-Xin , Wang Haifeng 
Published: November 2020

Abstract
Background: The olive extract contains compounds with antioxidant and anti-inflammatory properties. This study was designed to investigate whether olive cake extract, enriched with maslinic acid and hydroxytyrosol, alleviates the lipopolysaccharides (LPS)-induced oxidative stress, inflammation and intestinal villus damage in piglets.Methods: Thirty weaned piglets (6.9±0.9 kg) were assigned to five groups using a randomized complete block design. Piglets were fed a basal diet before intraperitoneal (i.p.) injection of physiological saline (C); fed a basal diet alone (CL) or fed a basal diet plus olive extract (OL), antibiotics (AL), or olive extract and antibiotics (OAL) before i.p. injection of LPS. The feeding lasted for 2 weeks. Piglets were euthanized 4h after LPS injection. Systemic anti-oxidant and inflammation levels were measured and villus morphology in the intestine was examined.Results: Compared with those in the C group, piglets in the CL group had significantly lower GSH-Px, SOD, ALB levels and higher MDA, NO, LDH, ALT and AST levels in the serum (P<0.05). Compared with the CL group, piglets in OL, AL, and OAL groups had significantly higher serum GSH-Px, SOD and ALB levels and lower MDA, NO, LDH, ALT and AST levels (P<0.05). LPS administration significantly increased the serum concentration of TNF-α, IL-6, DAO and D-xylose in the CL group compared with the control group (P<0.05). Piglets in OL, AL, and OAL groups had significantly lower serum TNF-α, IL-6, DAO and D-xylose levels and higher IL-10 level (P<0.05). In the duodenum and ileum of piglets, LPS challenge led to significantly lower villus height (VH), higher crypt depth (CD) and lower VH/CD compared with the control group (P<0.05), whereas, OL, AL, and OAL groups had significantly lower CD and higher VH/CD compared with the CL group (P<0.05). Dietary inclusion of olive extract increased the relative abundance of intestinal Lactobacillus and Clostridium at genus level.Conclusion: Dietary supplementation with olive extract maslinic acid and hydroxytyrosol improved anti-oxidative and anti-inflammatory capacity, intestinal structure morphology, and increased the abundance of beneficial intestinal bacteria in weaned piglets challenged by LPS.
KLF4, a Key Regulator of a Transitive Triplet, Acts on the TGF-β Signaling Pathway and Contributes to High-Altitude Adaptation of Tibetan Pigs
Authors: Wang Tao , Guo Yuanyuan , Liu Shengwei , Zhang Chaoxin , Cui Tongyan , Ding Kun , Wang Peng , Wang Xibiao , Wang Zhipeng 
Published: November 2020

Abstract
Background: Tibetan pigs are native mammalian species on the Tibetan Plateau that have evolved distinct physiological traits that allow them to tolerate high-altitude hypoxic environments. They can be used as a suitable animal model for exploring the molecular mechanism of hypoxia adaptation in high-altitude organisms.Results: Here, based on multi-tissue transcriptional data from high-altitude Tibetan pigs and low-altitude Rongchang pigs, we performed a weighted correlation network analysis (WGCNA) and identified key modules related to these tissues. Complex network analysis and bioinformatics analysis were integrated to identify key genes and size-3 network motifs. The results showed that compared to other tissues, the lungs of Tibetan pigs and Rongchang pigs are more significantly different, showing more adaptive transcriptional changes. In the lung tissues of Tibetan pigs, we identified KLF4, BCL6B, EGR1, EPAS1, SMAD6, SMAD7, KDR, ATOH8 and CCN1 genes as potential regulators of hypoxia adaption. We found that KLF4 and EGR1 genes simultaneously regulate the BCL6B gene, forming a KLF4-EGR1-BCL6B transitive triplet. This transitive triplet, dominated by KLF4, may enhance the hypoxia adaptability of Tibetan pigs by mediating the TGF-β signaling pathway. This triplet also regulates the KDR gene, which is involved in the PI3K-Akt signaling pathway and plays an important role in hypoxia adaptation. Conclusions: We postulate that the KLF4-EGR1-BCL6B transitive triplet may contribute to the adaptation of Tibetan pigs to hypoxic environments. These findings provide new details of the regulatory architecture of hypoxia-adaptive genes and are valuable for understanding the genetic mechanism of hypoxic adaptation in mammals.
An 192 bp ERV insertion in the first intron of TLR6 acts as an enhancer to increase TLR6 and TLR1 expression
Authors: wang xiaoyan , Chen Zixuan , Murani Eduard , D'alessandro Enrico , An Yalong , Chen Cai , Li Kui , Galeano Grazia , Wimmers Klaus , Song Chengyi 
Published: November 2020

Abstract
Background

Toll-like receptors (TLRs) play important roles in building innate immune and inducing adaptive immune responses. Associations of the TLR gene polymorphisms with diseases susceptibility, which are the basis of molecular breeding for disease resistant animals, have been reported extensively. Retrotransposon insertion polymorphisms (RIPs) were developed recently as a new type of molecular marker having great potential in population genetics and quantitative trait locus (QTL) mapping analysis. In this study, bioinformatics prediction combined with the PCR-based amplification was employed to screen for RIPs in porcine TLR genes. Their population distribution and impact on gene activity and phenotype of one RIP was further evaluated.
Results

Totally, five RIPs, located at the 3' flank of TLR3, 5' flank of TLR5, intron 1 of TLR6, intron 1 of TLR7, and 3' flank of TLR8 respectively, were identified. These RIPs were detected in different breeds with an uneven distribution among them. By using the dual luciferase activity assay a 192 bp endogenous retrovirus (ERV) in the intron 1 of TLR6 was proven to act as an enhancer increasing the activities of TLR6 promoter and multiple mini-promoters. Furthermore, the real-time quantitative polymerase chain reaction (qPCR) analysis demonstrated that the ERV insertion significantly enhances the mRNA expressions of TLR6, the neighboring gene TLR1, and the downstream genes MyD88 (Myeloid differentiation factor 88), Rac1 (Rac family small GTPase 1 ), TIRAP (TIR domain containing adaptor protein), Tollip (Toll interacting protein) of TLR signaling pathway and the inflammatory factors IL6 (Interleukin 6), IL8 (Interleukin 8), and TNFα (Tumor necrosis factor alpha) in 30-day piglet tissues. In addition, the serum IL-6 and TNFα were also significantly upregulated by ERV insertion.
Conclusions

Overall, five RIPs were identified in several TLRs, and the 192 bp ERV insertion in the first intron of TLR6 can improve the expressions of TLR6, TLR1, their downstream genes, and the inflammatory factors by acting as an enhancer affecting the regulation of TLR pathways, which may be applicable in the molecular breeding of disease resistant animals.
Treatment of Supraventricular Tachycardia in Patients with Non-Cardiac&nbsp;Surgery&nbsp;by Dexmedetomidine During the Perioperative&nbsp;Period. A Randomized Trial
Authors: Hu CuiWen , Xu Yan , Guo Xuan , Yang Shengnan , Zhang Qi , Zhang JunLong 
Published: November 2020

Abstract
Background: Previous studies have shown that application of dexmedetomidine (Dex) combined anesthesia during surgery can significantly reduce cardiovascular system complications and mortality of patients with cardiac disease during the perioperative period. The aim of this study was to explore the therapeutic effect of Dex on perioperative supraventricular tachycardia (SVT) in adult patients with non-cardiac surgery.Methods: Forty-six patients with SVT undergoing elective non-cardiac surgery were randomly divided into two groups, intravenously infused Dex (Dex group, 1.0 µg/kg) or midazolam (midazolam group, 0.06 mg/kg) for 10 minutes, respectively. The observation indexes containing the treatment efficiency of SVT, heart rate (HR) and and heart rate variability (HRV) including normalized low frequency power (LFnorm), normalized high frequency power (HFnorm) and LFnorm/HFnorm were recorded.Results: Treatment rates of SVT were 21/23 (91.3%) in Dex group vs 2/23 (8.7%) in midazolam group (P<0.001). In Dex group, LFnorm and LFnorm/HFnorm were decreased, and HFnorm were elevated and HR were decreased after twenty-three patients infused Dex (P < 0.05). However, there was no difference for HFnorm, LFnorm and LFnorm/HFnorm in midazolam group (P > 0.05). Conclusion: Perioperative use of dexmedetomidine has a significant therapeutic effect for SVT, and its mechanism is related to adjust cardiac autonomic nervous system and has no obvious connection with sedation.Trial registration: This trial was registered at ClinicalTrials.gov. registry number: NCT04284150 on February 13, 2020.