bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical impacts of total parenteral nutrition in hematopoietic stem cell transplantation patients with high nutritional risk
Authors: Yang Le , Wu Di , Dai Junting , Lv Huiyi , Li Miao 
Published: December 2024

BackgroundHematopoietic stem cell transplantation (HSCT) patients often receive consecutive intensive chemotherapy, which can lead to gastrointestinal complications and acute graft-versus-host disease (GVHD), placing patients at high nutritional risk.AimThis retrospective study aimed to evaluate the benefits of nutritional support in maintaining nutritional status, reducing weight loss without increasing the incidence of catheter-related bloodstream infections (CRBSI) or liver dysfunction, and improving clinical outcomes in HSCT patients at high nutritional risk.MethodsA total of 526 patients who underwent HSCT were included in the study. Based on the Nutrition Risk Screening-2002 (NRS-2002) and propensity score matching, 70 patients were assigned to the control group (without parenteral nutrition) and 70 to the enhanced nutrition group (with parenteral nutrition) between 2012 and 2022. We compared data between the two groups at different time points (days 3, 7, 10, and 14 after transplantation and the day before discharge) on the following: (1) effectiveness: weight loss, albumin, and prealbumin levels; (2) safety: incidence of CRBSI and conjugated bilirubin levels; and (3) clinical outcomes: hospital stay duration, rate of rehospitalization, hospitalization costs, and survival rates.ResultsOur results showed that total parenteral nutrition (TPN) effectively mitigated weight loss on days 10 and 14 and the day before discharge, while also improving albumin (33.41 ± 4.57 in the control group, 34.87 ± 4.08 in the TPN group, p &lt; 0.05; 33.72 ± 3.52 in the control group, 35.27 ± 4.04 in the TPN group, p &lt; 0.05; 34.09 ± 4.44 in the control group, 35.55 ± 3.87 in the TPN group, p &lt; 0.05) and prealbumin (245.18 ± 79.94 in the control group, 274.26 ± 86.73 in the TPN group, p &lt; 0.05; 233.27 ± 79.57 in the control group, 279.34 ± 80.20 in the TPN group, p &lt; 0.01; 247.24 ± 83.29 in the control group, 280.65 ± 100.22 in the TPN group, p &lt; 0.05) levels during the same periods. In addition, there were no significant differences in CRBSI incidence or liver function between the non-TPN and TPN groups. Furthermore, the TPN group experienced a shorter length of hospital stay (48.06 ± 13.90 in the control group, 42.13 ± 14.22* in the TPN group, p &lt; 0.05) and lower rates of unexpected rehospitalization (37.1% in the control group, 21.4% in the TPN group, p &lt; 0.05).ConclusionThis study demonstrated that effective TPN formulations improved nutritional status, ensured patient safety, and contributed to better clinical outcomes in HSCT patients at high nutritional risk. These findings support the use of nutritional interventions in hematologic malignancy patients receiving induction therapy prior to transplantation.
The deep learning radiomics nomogram helps to evaluate the lymph node status in cervical adenocarcinoma/adenosquamous carcinoma
Authors: Xiao Mei Ling , Fu Le , Qian Ting , Wei Yan , Ma Feng Hua , Li Yong Ai , Cheng Jie Jun , Qian Zhao Xia , Zhang Guo Fu , Qiang Jin Wei 
Published: December 2024

ObjectivesThe accurate assessment of lymph node metastasis (LNM) can facilitate clinical decision-making on radiotherapy or radical hysterectomy (RH) in cervical adenocarcinoma (AC)/adenosquamous carcinoma (ASC). This study aims to develop a deep learning radiomics nomogram (DLRN) to preoperatively evaluate LNM in cervical AC/ASC.Materials and methodsA total of 652 patients from a multicenter were enrolled and randomly allocated into primary, internal, and external validation cohorts. The radiomics features were extracted from axial T2-weighted imaging (T2WI), diffusion-weighted imaging (DWI), and contrast-enhanced T1-weighted imaging (CE-T1WI). The DL features from T2WI, DWI, and CE-T1WI were exported from Resnet 34, which was pretrained by 14 million natural images of the ImageNet dataset. The radscore (RS) and DL score (DLS) were independently obtained after repeatability test, Pearson correlation coefficient (PCC), minimum redundancy maximum relevance (MRMR), and least absolute shrinkage and selection operator (LASSO) algorithm performed on the radiomics and DL feature sets. The DLRN was then developed by integrating the RS, DLS, and independent clinicopathological factors for evaluating the LNM in cervical AC/ASC.ResultsThe nomogram of DLRN-integrated FIGO stage, menopause, RS, and DLS achieved AUCs of 0.79 (95% CI, 0.74–0.83), 0.87 (95% CI, 0.81–0.92), and 0.86 (95% CI, 0.79–0.91) in the primary, internal, and external validation cohorts. Compared with the RS, DLS, and clinical models, DLRN had a significant higher AUC for evaluating LNM (all P &lt; 0.005).ConclusionsThe nomogram of DLRN can accurately evaluate LNM in cervical AC/ASC.
Optimization of substrate formulation for Hericium erinaceus by replacing wood by straw and their effect on enzyme activities
Authors: Lu Zhu , Liu Lingyun , Ren Ziming , Hu Shuang , Wang Yue , Ji Shujuan , Wang Xu , Du Zhongwei , Liu Yanni , Yang Yang , Yu Yanshen 
Published: December 2024

IntroductionChina is rich in straw resources. The utilization of straw in the cultivation of edible fungi partially resolves the resource conflicts between mushroom cultivation and forest industry and also contributes to environmental protection.MethodsIn this study, based on the technology of replacing wood by grass, the straw formula for mycelial culture of Hericium erinaceus was optimized with Simplex-lattice method commonly used in mixture design. By measuring the growth rate and the activity of lignocellulose degrading enzymes of mycelia in different formulations, and further combining with model optimization, the optimal formulation was screened and validated for mushroom cultivation.ResultsIn the experiments, different kinds of straw used as the main material showed interaction effects, further affecting the growth rate of mycelia and the activities of laccase, cellulase, and neutral xylanase. The screened optimal formula was composed of 16.3% rice straw, 59.7% cob, 20.0% wheat bran, 2.0% gypsum, 1.0% sucrose, and 1.0% calcium superphosphate. In the mushroom cultivation, 445.69 g of fresh mushroom were obtained and the biological efficiency reached 89.14%. The growth period of the first mushroom was shortened by 7-9 days. Some nutritional components of fruiting bodies, such as crude fats (6.10%), crude proteins (152.02 g/kg), K (19.71 g/kg), P (2.48 g/kg), and Se (6.06 g/kg), were significantly higher than those of the control formula.DiscussionThese above indicators indicated that the screened formula could be applied in the high-yield and high-quality cultivation of H. erinaceus. Our study lays the foundation for expanding cultivation and strains improvement of H. erinaceus, and is conducive in promoting the rapid development of H. erinaceus industry.
Unveiling the molecular mechanism of sepal curvature in Dendrobium Section Spatulata through full-length transcriptome and RNA-seq analysis
Authors: Qu Xuefeng , Li Na , Xu Cong , Huang Zifeng , Li Chunyan , Jiang Yang , Zheng Guizhao , Fu Haiping , Zhang Guangyan , Liu Chuan 
Published: December 2024

IntroductionOrchids are renowned for their intricate floral structures, where sepals and petals contribute significantly to ornamental value and pollinator attraction. In Dendrobium Section Spatulata, the distinctive curvature of these floral organs enhances both aesthetic appeal and pollination efficiency. However, the molecular and cellular mechanisms underlying this trait remain poorly understood.MethodsMorphological characteristics of five hybrids were analyzed, with a particular focus on hybrid H5, which exhibits pronounced sepal curling. Full-length transcriptomic sequencing was employed to assemble a reference transcriptome, while RNA-seq identified differentially expressed genes (DEGs) between sepals and petals. Gene ontology and pathway enrichment analyses were conducted to uncover biological processes associated with sepal curvature. Cytological microscopy was used to examine cell size and number, and quantitative real-time PCR (qRT-PCR) was performed to validate transcriptomic findings.ResultsThe reference transcriptome contained 94,258 non-redundant transcripts, and RNA-seq identified 821 DEGs between sepals and petals, with 72.8% of these upregulated in sepals. Enrichment analysis revealed the significant involvement of DEGs in cytokinesis, cytoskeletal organization, and energy metabolism. Notably, myosin II filament organization was implicated in generating the mechanical forces responsible for curling, while metabolic pathways provided the energy necessary for these developmental processes. Cytological observations showed that the upper cell layers of the sepal were smaller and more numerous than the lower layers, indicating that differential cell growth contributes to sepal curvature. qRT-PCR analysis validated the differential expression of selected genes, supporting the transcriptomic findings.DiscussionThe interplay of cellular mechanics, cytoskeletal dynamics, and metabolic regulation is crucial in shaping sepal morphology. Future studies involving gene knockdown or overexpression experiments are recommended to validate the roles of specific genes in processes such as actin organization and myosin activity. Such work would provide deeper insights into the contributions of cytoskeletal dynamics and mechanical force generation to sepal morphogenesis.
Knowledge, perceptions, and management of symptoms of hormonal imbalance among adolescent girls in selected schools in Ghana: a qualitative exploratory study
Authors: Zakariah-Akoto Sawudatu , Abuaku Benjamin , Egbi Godfred , Klu Bismark Edem Kofi , Kyei-Baafour Eric , Ofori Michael Fokuo , Ahorlu Collins Stephen , Yeboah-Manu Dorothy 
Published: December 2024

IntroductionAdolescent girls are more sensitive to hormonal imbalance with major impact on their nutritional, reproductive, physical, psychosocial, and academic wellbeing. This study explored adolescent girls’ knowledge and perceptions of causes and management of symptoms of hormonal imbalance.Materials and methodsUsing a qualitative approach, focus group discussions were conducted with 116 assented in-school adolescent girls aged 10–19 years between 3rd and 19th October 2022. Two urban and two rural communities in two regions were purposively selected for the study. Using a convenient sampling approach, participants were recruited from Upper Primary, Junior, and Senior High schools. Data was analyzed thematically using both inductive and deductive approaches.ResultsSenior High School participants had a fair understanding of hormonal imbalance in both regions. Symptoms were perceived to include headaches, heavy and painful periods, and pimples, most of which participants alluded to experiencing. Perceived causes included natural process of adolescent growth, consumption of unhealthy diets, use of cosmetics and medications. Perceived reproductive effects included infertility, pregnancy disorders, breastfeeding challenges, and low sexual drive. Physiological effects included extreme fatigue, loss of appetite and impaired growth. Psychosocial and academic effects included mood swings/irritability, low self-esteem, poor inter-personal relationship, poor concentration in class and irregular school attendance. Female relatives and friends were mostly consulted for advice. Self-medication, dietary modification, physical activities, and personal hygiene were adopted to manage perceived symptoms. Self-medication was common, but physical activities and dietary modification were also adopted by most participants to manage symptoms.ConclusionIt is recommended that Ghana Education Service should formally incorporate hormonal-related issues into its School Health Education Programmes to enhance knowledge, attitudes, and management among adolescent girls at all levels of education.
Performance of the Verily Study Watch for measuring sleep compared to polysomnography
Authors: Saeb Sohrab , Nelson Benjamin W. , Barman Poulami , Verma Nishant , Allen Hannah , de Zambotti Massimiliano , Baker Fiona C. , Arra Nicole , Sridhar Niranjan , Sullivan Shannon S. , Plowman Scooter , Rainaldi Erin , Kapur Ritu , Shin Sooyoon 
Published: December 2024

IntroductionThis study evaluated the performance of a wrist-worn wearable, Verily Study Watch (VSW), in detecting key sleep measures against polysomnography (PSG).MethodsWe collected data from 41 adults without obstructive sleep apnea or insomnia during a single overnight laboratory visit. We evaluated epoch-by-epoch performance for sleep vs. wake classification, sleep stage classification and duration, total sleep time (TST), wake after sleep onset (WASO), sleep onset latency (SOL), sleep efficiency (SE), and number of awakenings (NAWK). Performance metrics included sensitivity, specificity, Cohen's kappa, and Bland-Altman analyses.ResultsSensitivity and specificity (95% CIs) of sleep vs. wake classification were 0.97 (0.96, 0.98) and 0.70 (0.66, 0.74), respectively. Cohen's kappa (95% CI) for 4-class stage detection was 0.64 (0.18, 0.82). Most VSW sleep measures had proportional bias. The mean bias values (95% CI) were 14.0 min (5.55, 23.20) for TST, −13.1 min (−21.33, −6.21) for WASO, 2.97% (1.25, 4.84) for SE, −1.34 min (−7.29, 4.81) for SOL, 1.91 min (−8.28, 11.98) for light sleep duration, 5.24 min (−3.35, 14.13) for deep sleep duration, and 6.39 min (−0.68, 13.18) for REM sleep duration. Mean and median NAWK count differences (95% CI) were 0.05 (−0.42, 0.53) and 0.0 (0.0, 0.0), respectively.DiscussionResults support applying the VSW to track overnight sleep measures in free-living settings. Registered at clinicaltrials.gov (NCT05276362).
Imaging and clinical outcomes of COVID-19- vs. non-COVID-19-related cerebral venous thrombosis
Authors: Maxhuni Toska , Doeppner Thorsten R. , Braun Tobias , Emde Julia , Struffert Tobias , Dembek Thomas , Huttner Hagen B. , Juenemann Martin B. , Gerner Stefan T. 
Published: December 2024

BackgroundCerebral venous thrombosis (CVT) is a rare but serious subtype of stroke. Several studies have reported an increased incidence of CVT after either COVID-19 (CoV19) infection or vaccination; however, data on clinical characteristics, the radiological profiles, and the outcomes of these patients with CVT as the only severe symptom of a CoV19 infection or vaccination compared to patients with non-CoV19-related CVT are still scarce.MethodsWe performed a retrospective monocentric study over 10 years (January 2013–December 2022) that included consecutive patients with a confirmed diagnosis of CVT based on imaging of the cerebral venous system. Patients were categorized as CoV19 CVT (either due to infection or post-vaccination) or non-CoV19 CVT and compared regarding demographics, risk factors, clinical characteristics, and imaging findings as well as outcome (at discharge, at 6 months, and last follow-up). Furthermore, sub-analyses were performed to compare CoV19-infection-related-CVT and CoV19-vaccination-related-CVT patients.ResultsOverall, 122 patients with suspected CVT were identified. After excluding patients with missing data (n = 20) or missing imaging of the cerebral venous system (n = 31), 71 patients with confirmed CVT remained for the final analyses. Of those, 11 patients had CoV19 CVT (infection n = 3, vaccination n = 8), and 60 patients had non-CoV19-CVT. There were no differences regarding median age (CoV19: 40 [IQR: 22–70] vs. non-CoV19: 41 [IQR:27–64]) or percentage of female sex among both groups. A lower rate of CVT risk factors was observed in the CoV19 group but without significant differences. No patient with CoV19 CVT displayed impaired consciousness on presentation, and only 30% had focal neurological deficits compared to 51.7% in the control group. The rate of CVT-related intracranial hemorrhage and venous infarcts were 27.3% and 9.1%, respectively, in the CoV19 group and 30% and 16.7%, respectively, in the non-CoV19 group. The mortality rate at discharge was 9.1% in the CoV19-CVT group vs. 3.3% in the non-CoV19-CVT group, without differences in functional outcomes during the follow-up period. Sub-analyses comparing CoV19-infection-related CVT vs. CoV19-vaccination-related CVT patients revealed no significant differences in clinical, imaging, or treatment characteristics.ConclusionIn this monocentric study, there was no signal for a worse severity of CoV19 CVT compared to non-CoV19 CVT regarding clinical characteristics, imaging profile, or outcomes in patients with CVT only. Larger observational data with sophisticated workups of CVT patients are needed to confirm our results.
The Drosophila toothrin Gene Related to the d4 Family Genes: An Evolutionary View on Origin and Function
Authors: Kuvaeva Elena E. , Cherezov Roman O. , Kulikova Dina A. , Mertsalov Ilya B. 
Published: December 2024

D. melanogaster has two paralogs, tth and dd4, related to the evolutionarily conserved d4 family genes. In mammals, the family consists of Dpf1-3, encoding transcription co-factors involved in the regulation of development and cell fate determination. The function of tth and dd4 in Drosophila remains unclear. The typical domain structure of the proteins encoded by the d4 family consists of an N-terminal 2/3 domain (Requiem_N), a central Kruppel-type zinc finger, and a C-terminal D4 domain of paired PHD zinc fingers (DPFs). In Drosophila, both paralogs lack the Kruppel-type ZF, and tth encodes a protein that contains only Requiem_N. In contrast, vertebrate Dpf1-3 paralogs encode all the domains, but some paralogs have specific splice isoforms. For example, the DPF3a isoform lacks the D4 domain necessary for histone reading. The occurrence of proteins without the D4 domain in mammals and flies implies functional significance and analogous roles across animal taxa. In this study, we reconstructed the evolutionary events that led to the emergence of Drosophila tth by analyzing the divergence of d4 paralogs across different evolutionary lineages. Our genomic and transcriptomic data analysis revealed duplications and gene copy loss events. Among insects, gene duplication was only observed in Diptera. In other lineages, we found the specialization of paralogs for producing isoforms and further specialization for coding proteins with specific domain organizations. We hypothesize that this pathway is a common mechanism for the emergence of paralogues lacking the D4 domain across different evolutionary lineages. We, thus, postulate that TTH may function as a splice isoform of the ancestral single-copy gene, possibly a DPF3a-like isoform characteristic of related insect species. Our analysis provides insights into the possible impact of paralogue divergence, emphasizing the functional significance of the 2/3 domain and the potential roles of isoforms lacking the D4 domain.
CUORE PARA A ESCOLA ELEMENTAR DOS DOIS LADOS DO ATLÂNTICO (ENTRE FINS DO SÉCULO XIX E INÍCIO DO SÉCULO XX)
Authors: Claudia Panizzolo Profa. Dra. , Ascenzi Anna 
Published: December 2024

Entre as décadas finais do século XIX e as primeiras décadas do século XX houve distribuição e circulação de livros italianos entre os imigrantes e seus descendentes. Silabários, livros de leitura, de religião, de aritmética, de história, de geografia, de cantos, entre outros que seguiam o programa curricular vigente na Itália, atravessaram o Atlântico para serem adotados nas escolas italianas em São Paulo. O objeto de investigação deste artigo, o centenário e ainda publicado Cuore, de Edmondo De Amicis, cuja primeira edição data de 1886, na Itália, para assim tornar-se um grande sucesso editorial ganhou traduções e foi adotado nas escolas paulistas. A intenção neste artigo é compreender, de um lado, as políticas educacionais que regraram a produção de livros escolares para as escolas italianas no exterior, e de outro lado, as motivações que levaram à circulação e a distribuição para escolas italianas e paulistas, entre fins do século XIX e início do século XX. Ancorado nos referenciais da História Cultural e História da Educação e mobilizando o corpus conceitual em especial de Chartier (1994,1996,1998, 2004) acerca da produção e representação foram definidos os procedimentos, os instrumentos, as fontes e as direções de análise. A análise documental toma como fonte privilegiada além do Cuore, a imprensa, os relatórios de cônsules, ofícios, despachos, circulares ministeriais e anuário das escolas italianas e da instrução pública paulista.
Особенности анатомии и морфологии системы корневых каналов премоляров нижней челюсти. Данные для управления рисками
Authors: Савостикова О.С. , Кронивец Н.А. , Мазнёва А.И. 
Published: December 2024

В данной работе было проведено исследование конусно-лучевых компьютерных томограмм для изучения особенностей анатомии и морфологии премоляров нижней челюсти, что позволяет повысить эффективность эндодонтического лечения и управление рисками.
In this work, a study of cone-beam computed tomograms was conducted to study the features of the anatomy and morphology of the premolars of the mandible, which makes it possible to increase the effectiveness of endodontic treatment.
Ultra-rapid cooling in the millisecond timescale for the enhancement of polarization properties in Al:HfO2 thin films using flash lamp annealing
Authors: Tanimura Hideaki , Mifune Tomoya , Ueno Yuma , Fujisawa Hironori , Nakashima Seiji , Osaka Ai I. , Kato Shinichi , Mikawa Takumi 
Published: December 2024

Abstract
Superior ferroelectric properties of Al-doped HfO2 (HAO) thin films are demonstrated using flash lamp annealing (FLA). This annealing approach is a low-thermal-budget treatment that features short annealing times at the millisecond timescale. We first clarified the annealing conditions with respect to optimum ferroelectricity. The results show that 5-millisecond annealing at 1000 °C is sufficient for adequate crystallization, achieving a high 2Pr value of 17.3 μC cm−2. By adjusting the cooling rate on the millisecond timescale during crystallization annealing, a high cooling rate of 182 °C ms−1 exhibited a superior 2Pr value of 16.6 μC cm−2, in contrast to a slow cooling rate of 12 °C ms−1, which yielded a 2Pr value of 10.2 μC cm−2. The results indicate that the control of the cooling rate is crucial for achieving an optimum 2Pr value, illustrating the potential of FLA for forming high-quality ferroelectric thin films.
Hubungan Customer Satisfaction dengan Customer Retention di Rumah Sakit Pupuk Kaltim Prima Sangatta
Authors: Andi Niartiningsih  , Munsir Noviani 
Published: December 2024

Objektif. Customer retention merupakan bentuk loyalitas yang berhubungan dengan perilaku yang diukur berdasarkan perilaku belikonsumen yang ditunjukkan dengan tingginya frekuensi konsumen membeli suatu produk. Customеr rеtеntion diаnggаp sеbаgаi sаlаh sаtu indikаtor pеnting customеr loyаlity dаn customеr sаtisfаction digunаkаn sеcаrа bеrgаntiаn yang bеrdаmpаk lаngsung pаdа nilаi pеlаnggаn dеngаn jаngkа wаktu yаng pаnjаng. Penelitian ini bertujuan untuk menganalisis hubungan customer satisfaction dengan customer retention di Rumah Sakit Pupuk Kaltim Prima Sangatta.
Metode Riset. Penelitian ini adalah jenis penelitian penelitian kuantitatif yang menggunakan studi observasional analitik dengan rancangan cross sectional study.
Hasil. Hasil penelitian menunjukkan ada hubungan customer satisfaction terhadap customer retention di Rumah Sakit Pupuk Kaltim Prima Sangatta. Hasil uji statistik dengan menggunakan uji chi square diperoleh nilai p= 0.000, karena nilai p<α = 0.000<0.05.

Kesimpulan. Berdasarkan penjelasan sebelumnya, dapat disimpulkan customer satisfaction merupakan kunci utama dalam mempertahankan pelanggan, karena kepuasan pelanggan menentukan apakah pelanggan tersebut akan tetap atau beralih ke rumah sakit lain.