bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knocking Down LncRNA-GAS5 Restores the Inhibition of Cuprotosis Protein FDX-1 by Acinetobacter baumannii
Authors: An Zhiyuan , Ding Wenyi 
Published: December 2024

Abstract
This study investigated the regulatory effect of LncRNA-GAS5 on FDX-1 in HEK293T cells by Acinetobacter baumannii. Transfected LncRNA-GAS5 overexpressing or knocking down plasmids into HEK293T cells, and the expression of FDX-1 was detected by Western blotting. A. baumannii inhibited the expression of FDX-1. Overexpression of LncRNA-GAS5 inhibited the expression of FDX-1, while knocking down LncRNA-GAS5 increased the expression of FDX-1. Overexpression of LncRNA-GAS5 further enhanced the inhibitory effect of A. baumannii on FDX-1, while knocking down LncRNA-GAS5 restored the inhibitory effect of A. baumannii on FDX-1. LncRNA-GAS5 regulates the inhibitory effect of A. baumannii on FDX-1 in HEK293T cells.
Advantages of Syndromic Diagnostics: Detection of the Pathogens Causing Urethritis/Cervicitis with the STI CNM Real-Time PCR Kit from Vitro S.A.
Authors: López Miguel Ángel Castaño , Porteros Héctor Toledo , De La Iglesia Salgado Alberto , Medel Josefa Vazquez , Borrero Inmaculada García 
Published: December 2024

Abstract
The STI CNM Real-Time PCR Kit from Vitro S.A. (Spain) demonstrates high sensitivity and specificity, is cost-effective, and can detect the three main etiological agents of urethritis/cervicitis in a single multiplex PCR. Sexually transmitted infections (STIs) are a significant public health problem and a significant burden of morbidity and mortality in hospitals. The World Health Organization (WHO) estimates the number of daily infections to be 1 million. Currently, the number of infections and antimicrobial-resistant strains is rising. A rapid and accurate etiologic diagnosis is critical to solving this problem. In this study, we compared the STI CNM Real-Time PCR Kit using the Xpert® CT/NG technique (Cepheid®, USA) as Gold Standard for the diagnosis of Chlamydia trachomatis and Neisseria gonorrhoeae and EasyNAT® MG (Ustar Biotechnologies (Hangzhou) Ltd., China) as Gold Standard for the diagnosis of Mycoplasma genitalium infection. Regarding C. trachomatis and N. gonorrhoeae, out of 200 samples, there was a match in 199 cases, with only one positive sample not being detected by the STI CNM Real-Time PCR Kit. This results in a sensitivity of 96% and a specificity of 100% for this kit. Diagnosing M. genitalium infection, out of 200 samples, the STI CNM Real-Time PCR Kit correctly detected all negative and positive samples, with 100% agreement compared to the reference technique. In summary, the STI assay has a very high sensitivity and specificity, comparable to other commercial diagnostic kits. Furthermore, it has the advantage of bundling the detection of the three main bacterial agents of urethritis/cervicitis, resulting in better cost efficiency.
Knowledge Sharing and Its Impact on Organizational Performance- An Analytical Study in the Directorate of Education, AL- Karkh First, Baghdad
Authors: Hafedh Farhan Mohammed , Yaseen Raheem Mohammed 
Published: December 2024

Purpose: The current research aims to identify the impact of knowledge sharing on organizational performance with a sample in the Ministry of Education/ the Directorate of Education of First Karkh.
Theoretical framework: Knowledge sharing is one of the key factors that contribute to improving organizational performance through exchanging of knowledge and experiences among employees. It highlights the importance of this process in developing and improving performance in a changeable environment.
Design/methodology/approach: This study was conducted in the Directorate of Education, First Karkh, Baghdad, with 187 legitimate questionnaires that were analyzed using superior statistical techniques.
Findings: Knowledge sharing significantly influences organizational performance and confirms both main hypotheses and sub-hypotheses.
Research, Practical & Social implications: Recommendations include improving training programs, encouraging collaboration, motivating employees through rewards, and encouraging technology sharing.
Originality/value: This research emphasizes the important role of knowledge sharing in improving organizational performance within the business sector. It provides valuable insights into how e adopting knowledge sharing practices can strengthen productivity and crisis management in the Iraqi context.
Increased hip fracture risk in the patients with type 2 diabetes mellitus is correlated with urine albumin-to-creatinine ratio (ACR) and diabetes duration in men
Authors: Ding Huiru , Wang Hongxia , Liu Guanghui , Wang Yu , Han Dongxu , Zhang Xiaoya , Song Lige 
Published: December 2024

Patients with type 2 diabetes mellitus (T2DM) have increased hip fracture risk. And the association between urine albumin to creatinine ratio (ACR) and an increased risk of hip fracture in patients with T2DM remains controversial. This study aimed to investigate the association between urinary ACR and hip fracture risk in postmenopausal women and aged men with T2DM. The study included 219 postmenopausal women and 216 older men (mean age &gt;60 years) with T2DM. Women and men were divided into control group (ACR&lt;30 mg/g), microalbuminuria group (30 mg/g ≤ ACR&lt;300 mg/g), and macroalbuminuria group (ACR≥300 mg/g) respectively. Demographic characteristics and clinical history were collected in patients. Biochemical indexes and bone turnover-related markers were measured in patients. In the study, we found that several factors, including age, T2DM duration, cerebral infarction history, serum corrected calcium levels and urine ACR were positively associated with hip fracture risk. However, 25-Hydroxyvitamin D and areal BMD were negatively associated with hip fracture risk. Furthermore, multiple regression analysis showed that urinary ACR level (β = 0.003, p = 0.044) and duration of T2DM (β = 0.015, p = 0.018) were positively and independently correlated with hip fracture risk in older men. In contrast, femoral neck BMD (β = −6.765, p &lt; 0.001) was independently and negatively correlated with hip fracture risk in older men. This study indicated that the elevated ACR levels and longer T2DM duration were related to higher hip fracture risk in older men with T2DM, which could be beneficial for developing a predictive model for osteoporotic fractures in patients with type 2 diabetes in the future. However, results were inconsistent in women, hip fracture risk didn’t alter by changes in urinary microalbuminuria level in postmenopausal women with T2DM.
Unveiling the molecular landscape of PCOS: identifying hub genes and causal relationships through bioinformatics and Mendelian randomization
Authors: He Yifang , Wang Yanli , Wang Xiali , Deng Shuangping , Wang Dandan , Huang Qingqing , Lyu Guorong 
Published: December 2024

BackgroundPolycystic ovary syndrome (PCOS) is a complex endocrine disorder with various contributing factors. Understanding the molecular mechanisms underlying PCOS is essential for developing effective treatments. This study aimed to identify hub genes and investigate potential molecular mechanisms associated with PCOS through a combination of bioinformatics analysis and Mendelian randomization (MR).MethodsThis study employed bioinformatics analysis in conjunction with MR methods using publicly available databases to identify hub genes. We employed complementary MR methods, including inverse-variance weighted (IVW), to determine the causal relationship between the hub genes and PCOS. Sensitivity analyses were performed to ensure results reliability. Enrichment analysis and immune infiltration analysis were further conducted to assess the role and mechanisms of hub genes in the development of PCOS. Additionally, we validated hub gene expression in both an animal model and serum samples from PCOS patients using qRT-PCR.ResultsIVW analysis revealed significant associations between 10 hub genes and the risk of PCOS: CD93 [P= 0.004; OR 95%CI= 1.150 (1.046, 1.264)], CYBB [P= 0.013; OR 95%CI= 1.650 (1.113,2.447)], DOCK8 [P= 0.048; OR 95%CI= 1.223 (1.002,1.494)], IRF1 [P= 0.036; OR 95%CI= 1.343 (1.020,1.769)], MBOAT1 [P= 0.033; OR 95%CI= 1.140 (1.011,1.285)], MYO1F [P= 0.012; OR 95%CI= 1.325 (1.065,1.649)], NLRP1 [P= 0.020; OR 95%CI= 1.143 (1.021,1.280)], NOD2 [P= 0.002; OR 95%CI= 1.139 (1.049,1.237)], PIK3R1 [P= 0.040; OR 95%CI= 1.241 (1.010,1.526)], PTER [P= 0.015; OR 95%CI= 0.923 (0.866,0.984)]. No heterogeneity and pleiotropy were observed. Hub genes mainly enriched in positive regulation of cytokine production and TNF signaling pathway, and exhibited positive or negative correlations with different immune cells in individuals with PCOS. qRT-PCR validation in both the rat model and patient serum samples confirmed hub gene expression trends consistent with our combined analysis results.ConclusionsOur bioinformatics combined with MR analysis revealed that CD93, CYBB, DOCK8, IRF1, MBOAT1, MYO1F, NLRP1, NOD2, PIK3R1 increase the risk of PCOS, while PTER decreases the risk of PCOS. This discovery has implications for clinical decision-making in terms of disease diagnosis, prognosis, treatment strategies, and opens up novel avenues for drug development.
A clinical practical model for preoperative prediction of visual outcome for pituitary adenoma patients in a retrospective and prospective study
Authors: Zheng Zijian , Wang He , Chen Qianxi , Wang Zhicheng , Fu Jun , Fan Wenjian , Lin Yuanxiang , Kang Dezhi , Jiang Changzhen , Lin Zhangya , Yan Xiaorong 
Published: December 2024

ObjectivePreoperative prediction of visual recovery after pituitary adenoma resection surgery remains challenging. This study aimed to investigate the value of clinical and radiological features in preoperatively predicting visual outcomes after surgery.MethodsPatients undergoing endoscopic transsphenoidal surgery (ETS) for pituitary adenoma were included in this retrospective and prospective study. Preoperative MRI, visual acuity, visual field, and postoperative visual recovery data were collected. Logistic regression analysis was used to assess the importance of clinical and MRI features, and a prediction model was developed.ResultsThe cohort included 198 patients (150 retrospective, 48 prospective). In the retrospective data, visual recovery was observed in 111 patients (74.0%), while non-recovery was observed in 39 patients (26.0%). In the prospective data, visual recovery was observed in 27 patients (56.25%) and non-recovery in 21 patients (43.75%). Blindness, headache, adenoma area, and adenoma upward growth distance were negatively correlated with visual recovery (p &lt; 0.05), while the pituitary gland area was positively correlated (p = 0.001). Logistic regression selected three clinical features: blindness, headache, and visual impairment course. Two additional imaging features, pituitary gland maximum area, and adenoma maximum area, were incorporated into the prediction model. The area under the curve of the prediction model was 0.944 in the retrospective cohort and 0.857 in the prospective cohort. Accuracy was 88% and 81.25%, respectively.ConclusionThis study successfully developed a clinical practical model combining clinical and radiological features to preoperatively predict visual recovery for patients with pituitary adenoma. The model has the potential to provide personalized counseling for individual patients.
Mechanism and application prospect of ferroptosis inhibitors in improving osteoporosis
Authors: Wang Jing , Chen TingRui , Gao Fei 
Published: December 2024

Osteoporosis, a prevalent bone metabolic disorder, has emerged as a pressing global public health concern. Recent studies have illuminated a crucial link between ferroptosis and the pathogenesis of osteoporosis. Nevertheless, the intricate mechanisms underlying the role of ferroptosis in this condition remain largely unexplored. Therefore, this article comprehensively reviews the regulatory mechanisms of ferroptosis and the modulating effects on the development and progression of osteoporosis, as reported in recent years. Furthermore, this review summarizes the current state of the interventional strategies employed by both Western medicines and traditional Chinese medicines (TCMs) in addressing osteoporosis. This review aims to uncover potential novel avenues for the prevention and control of osteoporosis by synthesizing the modes of action and clinical efficacy of these therapeutic modalities.
Abscopal effect of focused ultrasound combined immunotherapy in animal solid tumor model: a systematic reviews and meta-analysis
Authors: Hu Chao , Li Hui , Deng Tao , Liu Zheng , Yang Li , Peng Li , Jiang Ming Yan , Chen Wen Zhi 
Published: December 2024

IntroductionThe abscopal effect, a systemic anti-tumor response triggered by localized treatment, has gained attention but remains poorly understood. This study evaluates the efficacy and consistency of focused ultrasound (FUS) combined with immunotherapy in inducing the abscopal effect.MethodsA systematic review and meta-analysis were conducted on preclinical studies using solid tumor models. Data on tumor response, immune modulation, and survival outcomes were analyzed to assess the combination therapy's effectiveness.ResultsFUS combined with immunotherapy enhanced anti-tumor responses at local and distant sites, with evidence of immune activation and increased abscopal effect rates. However, heterogeneity across tumor models and protocols was observed.DiscussionThe findings provide a theoretical basis for FUS-immunotherapy combinations in cancer treatment, while emphasizing the need for standardized protocols and further research to elucidate underlying mechanisms.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42023460710.
Frequency, characteristics, and immunological accompaniments of ataxia in anti-NMDAR antibody-associated encephalitis
Authors: Jesse Sarah , Riemann Marie , Schneider Hauke , Ringelstein Marius , Melzer Nico , Vogel Niklas , Pfeffer Lena Kristina , Friese Manuel A. , Sühs Kurt-Wolfram , Hudasch Dominica , Schwenkenbecher Philipp , Günther Albrecht , Geis Christian , Wickel Jonathan , Lesser Martin , Kather Annika , Leypoldt Frank , Dargvainiene Justina , Markewitz Robert , Wandinger Klaus-Peter , Thaler Franziska S. , Kuchling Joseph , Wurdack Katharina , Sabater Lidia , Finke Carsten , Lewerenz Jan 
Published: December 2024

IntroductionVery rarely, adult NMDAR antibody-associated encephalitis (NMDAR-E) leads to persistent cerebellar atrophy and ataxia. Transient cerebellar ataxia is common in pediatric NMDAR-E. Immune-mediated cerebellar ataxia may be associated with myelin oligodendrocyte glycoprotein (MOG), aquaporin-4 (AQP-4), kelch-like family member 11 (KLHL11), and glutamate kainate receptor subunit 2 (GluK2) antibodies, all of which may co-occur in NMDAR-E. Here, we aimed to investigate the frequency, long-term outcome, and immunological concomitants of ataxia in NMDAR-E.MethodsIn this observational study, patients with definite NMDAR-E with a follow-up of &gt;12 months were recruited from the GENERATE registry. Cases with documented ataxia were analyzed in detail.ResultsIn 12 of 62 patients (19%), ataxia was documented. Bilateral cerebellar ataxia without additional focal CNS findings was found in four (one child and three adults); one of these was previously reported as a case with persistent cerebellar atrophy and ataxia. Two patients with bilateral cerebellar ataxia had additional focal neurological symptoms, optic neuritis and facial palsy. Two patients developed hemiataxia: one with diplopia suggesting brainstem dysfunction and the other probably resulting from cerebellar diaschisis due to contralateral status epilepticus. In all but the one developing cerebellar atrophy, cerebellar ataxia was transient and not associated with a worse long-term outcome. In all five patients with cerebellar ataxia tested, MOG, AQP-4, GluK2, and KLHL11 antibodies were negative. In two additional patients negative for both MOG and AQP-4 antibodies, ataxia was sensory and explained by cervical myelitis as part of multiple sclerosis (MS) manifesting temporal relation to NMDAR-E. One of the patients with bilateral ataxia with focal neurological deficits was also diagnosed with MS upon follow-up. Finally, in two patients, ataxia was explained by cerebral hypoxic damage following circulatory failure during an ICU stay with severe NMDAR-E.DiscussionAtaxia of different types is quite common in NMDAR-E. Cerebellar ataxia in NMDAR-E is mostly transient. NMDAR-E followed by persistent ataxia and cerebellar atrophy is very rare. Cerebellar ataxia in NMDAR-E may not be explained by concomitant KLHL11, MOG, AQP-4, or GluK2 autoimmunity. Of note, ataxia in NMDAR-E may result from treatment complications and, most interestingly, from MS manifesting in temporal association with NMDAR-E.
Relationship between triglyceride glucose-body mass index and coronary artery calcium score in maintenance hemodialysis patients
Authors: Jiang Zexi , Zhu Jinhua , Ding Hong , Yan Lihong , Chen Ruoxin , Wang Bin , Li ZuoLin , Liu Hong 
Published: December 2024

BackgroundThis study investigated the association between coronary artery calcification (CAC) and triglyceride glucose-body mass index (TyG-BMI) in patients receiving maintenance hemodialysis (MHD).MethodsWe used computed tomography (CT) to assess coronary artery calcification score (CACS) using the Agatston method. The TyG index was multiplied by BMI to derive the TyG-BMI index. Ordinal logistic regression models were used to analyze the relationship between TyG-BMI and CAC. The dose-response relationship was evaluated using restricted cubic spline regression. Weighted Quantile Sum regression was used to explore the weight of the TyG-BMI index components.ResultsBased on the TyG-BMI, 219 patients with MHD were stratified into three groups. The TyG-BMI index was shown to be an independent risk factor for CACS by multivariate ordinal logistic regression analysis (odds ratio, 1.011; [95% confidence interval, 1.002–1.021]; P = 0.021). The relationship between TyG-BMI and lg (CACS + 10) was linear (P-overall = 0.023, P-non-linear = 0.412). Body mass index (BMI) had the highest weight (0.566) when weights were assigned to the three components of TyG-BMI. In the non-diabetes and diabetes subgroups, TyG-BMI and lg (CACS + 10) did not exhibit a significant non-linear relationship.ConclusionTyG-BMI and CAC were independently positively correlated in patients undergoing MHD. These findings suggest that assessing TyG-BMI as a valuable tool for identifying the risk of CAC in patients with MHD.
Transcriptomic and metabolomic analyses reveal the spatial role of carnitine metabolism in the progression of hepatitis B virus cirrhosis to hepatocellular carcinoma
Authors: Gao Pengxiang , Liu Qiuping , Luo Ziye , Pu Wenjun 
Published: December 2024

IntroductionLiver cirrhosis (LC) and hepatocellular carcinoma (HCC) resulting from chronic hepatitis B virus (HBV) infection are major health concerns. Identifying critical biomarkers and molecular targets is needed for early diagnosis, prognosis, and therapy of these diseases.MethodsIn this study, we explored the gene expression and metabolism in the liver tissues of LC, HCC, and healthy controls, to analyse and identify potential biomarkers of disease progression. Mass spectrometry imaging was used to evaluate the spatial distribution of key metabolites.Results and discussionThe results revealed significant changes in gene expression and metabolic pathways along with disease progression. The upregulated genes were associated with extracellular matrix remodeling and cancer pathways, including LAMC1-3, COL9A2, COL1A1, MYL9, MYH11, and KAT2A. The downregulated genes were linked to immune response and fatty acid metabolism. Metabolomic analysis showed major changes in lipid and choline metabolism. Consistent changes in the expression of specific genes and metabolites were correlated with clinical data. Notably, metabolites such as L-acetylcarnitine, histamine, and 4-trimethylammoniobutanoic acid demonstrated high accuracy (AUC &gt; 0.85) in distinguishing between healthy, LC, and HCC groups. This study identifies key gene and metabolite changes in HBV related LC and HCC, highlighting critical pathways involved in disease progression. Biomarkers like L-acetylcarnitine and KAT2A show promise for early diagnosis and prognosis, potentially improving outcomes for hepatitis liver disease patients.
Differential brain activity in patients with disorders of consciousness: a 3-month rs-fMRI study using amplitude of low-frequency fluctuation
Authors: Qin Xuewei , Chen Xuanling , Yao Lan , Lu Fa , Liang Zhenhu , He Jianghong , Guo Xiangyang , Li Xiaoli 
Published: December 2024

IntroductionDisorders of consciousness (DoC) from severe brain injuries have significant impacts. However, further research on nuanced biomarkers is needed to fully understand the condition. This study employed resting-state functional MRI (rs-fMRI) and the amplitude of low-frequency fluctuation (ALFF) to investigate differential brain activity in patients with DoC following spinal cord stimulation (SCS) therapy. It also assessed the predictive value of rs-fMRI and ALFF in determining the consciousness levels at 3 months post-therapy.MethodsWe analyzed rs-fMRI data from 31 patients with traumatic brain injury (TBI) and 22 with non-traumatic brain injury (non-TBI) diagnosed with DoC. ALFF was measured before SCS therapy, and clinical outcomes were assessed 3 months later using the Coma Recovery Scale-Revised.ResultsPatients with TBI showed increased ALFF in the thalamus and anterior cingulate cortex, whereas the middle occipital lobe showed decreased ALFF. In the non-TBI group, a higher ALFF was noted in the precuneus, with a reduced ALFF in the occipital and temporal lobes. Patients with improved consciousness post-SCS exhibited distinct ALFF patterns compared with those with unchanged consciousness, particularly in the posterior cingulate and occipital regions.ConclusionThe application of ALFF in rs-fMRI may be a predictive tool for post-treatment outcomes in patients with DoC of varying etiologies. Differential ALFF in specific brain regions could indicate the likelihood of improvement in consciousness following SCS therapy.Clinical trial registrationhttps://www.chictr.org.cn/, Identifier ChiCTR2300069756.