bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retracted: Dexmedetomidine alleviates neuropathic pain by regulating JAK/STAT pathway in rats
Authors: Xun Shining , Zheng Rongzhi 
Published: November 2019

AbstractNeuropathic pain (NPP) is an unfavorable pathological pain with the characteristics of hyperalgesia, allodynia, and spontaneous pain. This study aimed to study the influence of dexmedetomidine (Dex) on NPP. Chronic constriction injury (CCI) was operated to form the NPP rat model. The OX42 and anti‐glial fibrillary acidic protein (GFAP), the nerve growth factors (NGFs), and the Janus kinase/signal transducers and activators of transcription (JAK/STAT) proteins were separately detected by quantitative reverse transcription‐polymerase chain reaction or Western blot. Inflammatory factors were detected by enzyme‐linked immunosorbent assay. The results demonstrated that Dex obviously alleviated CCI‐stimulated mechanical allodynia and thermal hyperalgesia. Meanwhile, the expressions of interleukin‐1β, tumor necrosis factor‐α, chemokine c‐X3‐c‐motif ligand 1, and C‐C motif chemokine ligand 2 were greatly increased in CCI rats, but these effects were reversed by Dex. In addition, Dex promoted the expressions of NGF, brain‐derived neurotrophic factor, neurotrophins‐3 (NT‐3), and NT‐4 in CCI rats. Moreover, the RNA or protein expression levels of OX42 and GFAP were significantly increased in CCI rats, while Dex inhibited the expressions of OX42 and GFAP. Furthermore, Dex blocked JAK/STAT signaling pathway by decreasing p‐JAK and p‐STAT in CCI rats. Dex had the potential to alleviate NPP by regulating JAK/STAT pathway in CCI rat.
MicroRNA‐598 acts as an inhibitor in retinoblastoma through targeting E2F1 and regulating AKT pathway
Authors: Liu Fengge , Zhang Qi , Liang Yan 
Published: November 2019

AbstractRecently, microRNAs (miRNAs) receive more attention due to their role in the pathogenesis of malignancies. Retinoblastoma (RB) is the most serious and harmful malignant tumor in infants and young children with eye diseases, which often endangers the lives of children. This study was designed to determine how miR‐598 is involved in RB progression. In this study, quantitative reverse transcription‐polymerase chain reaction, Western blot, dual‐luciferase reporter, Cell Counting Kit‐8, and Transwell assays were adopted to detect miR‐598 expression and function in RB. The decreased expression of miR‐598 was identified in RB. Overexpression of miR‐598 suppressed the viability and metastasis of RB cells. Further, E2F transcription factor 1 (E2F1) is verified as a direct target of miR‐598. Furthermore, E2F1 recovered miR‐598‐mediated‐inhibition of cell viability and metastasis in RB. In addition, miR‐598 was found to promote cell apoptosis and inactivate the protein kinase B (AKT) pathway in RB. miR‐598 suppressed RB cell viability and metastasis through inhibiting E2F1 and inactivating AKT pathway, which may provide a new perspective for RB treatment.
MiR‐3691‐5p is upregulated in docosahexaenoic acid‐treated vascular endothelial cell and targets serpin family E member 1
Authors: Wu Yutang , Chen Zhizhao , Wang Yaoguo , Peng Fangzhan 
Published: November 2019

AbstractEndothelium, the inner cellular lining of blood vessels, has an important role in the regulation of physiological processes and its dysfunction may initiate cardiovascular complications. Previous investigations have revealed that dietary docosahexaenoic acid (DHA) is related to a lower possibility of cardiovascular disease and mortality. Until now, the molecular mechanisms in the biological activities of DHA remain largely unknown. MicroRNAs (miRNAs) play a vital role in regulating gene expression. Thus, we aimed to investigate whether DHA improves the dysfunction via regulating miRNAs. To understand the protective effects of DHA through modulating miR‐3691‐5p and its target genes for palmitic acid (PAL) induced apoptosis in endothelial cells. The present study demonstrated that DHA upregulated miR‐3691‐5p expression, and downregulated the expression of its target gene serpin family E member 1 (SERPINE1). MiR‐mimics and inhibitors modulation results indicated that miR‐3691‐5p regulates endothelial apoptosis through activating antiapoptotic response which controlled by the STAT3 signaling pathway. In conclusion, we have shown that PAL‐induced apoptosis could be decreased by DHA treatment through miR‐3691‐5/SERPINE1 pathways.
Biochemical properties and progress in cancers of tRNA‐derived fragments
Authors: Ma Zhongliang , Zhou Jinbao , Shao Yang , Jafari Fatemah.A. , Qi Pengfei , Li Yanli 
Published: November 2019

AbstracttRNA‐derived small RNAs (tRFs), a kind of noncoding RNAs, are generated from transfer RNAs. tRFs have some types according to their source and sizes. They play important roles in cell life and carcinogenesis. In this paper, we review the biogenesis and biological properties. We also focus on current progress of tRFs and some tsRNAs such as tRF‐Leu‐CAG, which have been studied or will be further investigated in tumorgenesis and diagnostic biomarkers in the clinic.
New lower bounds for partial k‐parallelisms
Authors: Zhang Tao , Zhou Yue 
Published: November 2019

AbstractDue to the applications in network coding, subspace codes and designs have received many attentions. Suppose that and is an ‐dimensional space over the finite field . A ‐spread is a ‐set of ‐dimensional subspaces of such that each nonzero vector is contained in exactly one element of it. A partial ‐parallelism in is a set of pairwise disjoint ‐spreads. As the number of ‐dimensional subspaces in is , there are at most spreads in a partial ‐parallelism. By studying the independence numbers of Cayley graphs associated to a special type of partial ‐parallelisms in , we obtain new lower bounds for partial ‐parallelisms. In particular, we show that there exist at least pairwise disjoint ‐spreads in .
Reference intervals established using indirect method for serum ferritin assayed on Abbott Architect i2000SR analyzer in Chinese adults
Authors: Wang Qing‐ping , Guo Lin‐ying , Lu Zhi‐yong , Gu Jian‐wen 
Published: November 2019

AbstractBackgroundSerum ferritin (SF) test has been widely used in clinical practice. However, its reference intervals (RIs) vary depending on the analytical method and ethnic origin. This study was to establish the RIs using indirect method for SF in Chinese adults.MethodsSF was assayed on Abbott i2000SR analyzer. The SF test results of all health examinees (8913 males aged 18‐93 years and 5397 females aged 18‐90 years) between December 2010 and April 2019 were obtained from our laboratory information system. After Box‐Cox transformation of raw data and exclusion of outliers, parametric and non‐parametric approaches were used to calculate 95% RIs. The correlation between SF levels and ages, and the differences in SF levels between subgroups were also analyzed.ResultsSF levels in females were significantly different from those in males (Z = 88.96, Z* = 23.17; Z > Z*) and showed a weak positive correlation with age (r = .466, P < .0001). The RIs based on parametric approach in males were 66.12‐561.58 µg/L, whereas in all females were 3.59‐269.59 µg/L, females aged <50 years 3.26‐148.02 µg/L and those aged ≥50 years 17.28‐303.27 µg/L. The RIs based on non‐parametric approach in males were 65.00‐571.37 µg/L whereas in all females were 4.00‐254.00 µg/L, females aged <50 years 4.00‐152.00 µg/L and those aged ≥50 years 16.00‐304.05 µg/L.ConclusionsOur indirect RIs for SF were markedly different from the manufacturer's recommended RIs and might be more suitable for Chinese adults, which would be helpful in interpreting laboratory data and clinical decision‐making.
MiR‐142‐3p functions as a tumor suppressor by targeting RAC1/PAK1 pathway in breast cancer
Authors: Xu Tao , He Bang‐Shun , Pan Bei , Pan Yu‐Qin , Sun Hui‐Ling , Liu Xiang‐Xiang , Xu Xue‐Ni , Chen Xiao‐Xiang , Zeng Kai‐Xuan , Xu Mu , Wang Shu‐Kui 
Published: November 2019

AbstractMicroRNA‐142‐3p (miR‐142‐3p) was previously investigated in various cancers, whereas, it's role in breast cancer (BC) remains far from understood. In this study, we found that miR‐142‐3p was markedly decreased both in cell lines and BC tumor tissues. Elevated miR‐142‐3p expression suppressed growth and metastasis of BC cell lines via gain‐of‐function assay in vitro and in vivo. Mechanistically, miR‐142‐3p could regulate the ras‐related C3 botulinum toxin substrate 1 (RAC1) expression in protein level, which simultaneously suppressed the epithelial‐to‐mesenchymal transition related protein levels and the activity of PAK1 phosphorylation, respectively. In addition, rescue experiments revealed RAC1 overexpression could reverse tumor‐suppressive role of miR‐142‐3p. Our results showed miR‐142‐3p could function as a tumor suppressor via targeting RAC1/PAK1 pathway in BC, suggesting a potent therapeutic target for BC treatment.
Off‐line comprehensive two‐dimensional reversed‐phase countercurrent chromatography with high‐performance liquid chromatography: Orthogonality in separation of Polygonum cuspidatum Sieb. et Zucc
Authors: Wang Xiang , Zhao Shanshan , Wang Chaoyue , Sun Wenyu , Jin Yang , Gong Xingchu , Tong Shengqiang 
Published: November 2019

AbstractOff‐line comprehensive two‐dimensional reversed‐phase countercurrent chromatography with high‐performance liquid chromatography was investigated in separation of crude ethanol extract from traditional Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc. Two‐dimensional contour plots for countercurrent chromatography with high‐performance liquid chromatography was obtained after comprehensive separation was completed. Total peak capacity was evaluated and approximately 810 peaks were obtained through a comprehensive two‐dimensional separation. A highly orthogonality of 52.23% and a large separation space occupancy of 88.86% were achieved. Meanwhile, it was found that several components could be well separated by countercurrent chromatography while they could not be separated by high‐performance liquid chromatography, and vice versa, which further indicated the orthogonality of the two separation methods. The off‐line comprehensive two‐dimensional countercurrent chromatography with high‐performance liquid chromatography provided a promising and powerful method for separation of complex natural products.
Functional brand qualities and perceived value: The mediating role of brand experience and brand personality
Authors: Coelho Filipe J. F. , Bairrada Cristela M. , de Matos Coelho Arnaldo F. 
Published: November 2019

AbstractBrand experience and brand personality have become two important theoretical constructs in the branding literature. However, research on the antecedents of these two constructs has been focused on intangible brand characteristics and qualities, underestimating the role of functional features. This study aims to overcome this gap by postulating that two key functional brand qualities, quality and innovativeness, help shape brand experience and personality, which, we ultimately predict, contribute to perceived value. Investments in quality and innovation have grown substantially, but how they materialize in benefits for customers and firms remains unclear. Accordingly, this study provides insights into how firms can engender the characteristics of their offer to build brands that are perceived by consumers as generating a valued brand experience and personality. The results from two samples provide broad support to the proposed model. Both brand quality and innovativeness contribute to brand experience and personality. Moreover, we observe that both brand experience and personality relate to perceived value. Finally, we also determined that brand personality and experience partially mediate the relationship between brand innovativeness and quality and perceived value. Hence, these results provide relevant implications for both theory and the practice of brand management.
Syntaxin 6‐mediated exosome secretion regulates enzalutamide resistance in prostate cancer
Authors: Peak Taylor C. , Panigrahi Gati K. , Praharaj Prakash P. , Su Yixin , Shi Lihong , Chyr Jacqueline , Rivera‐Chávez José , Flores‐Bocanegra Laura , Singh Ravi , Vander Griend Donald J. , Oberlies Nicholas H. , Kerr Bethany A. , Hemal Ashok , Bitting Rhonda L. , Deep Gagan 
Published: November 2019

AbstractProstate cancer (PCa) deaths are typically the result of metastatic castration‐resistant PCa (mCRPC). Recently, enzalutamide (Enz), an oral androgen receptor inhibitor, was approved for treating patients with mCRPC. Invariably, all PCa patients eventually develop resistance against Enz. Therefore, novel strategies aimed at overcoming Enz resistance are needed to improve the survival of PCa patients. The role of exosomes in drug resistance has not been fully elucidated in PCa. Therefore, we set out to better understand the exosome's role in the mechanism underlying Enz‐resistant PCa. Results showed that Enz‐resistant PCa cells (C4‐2B, CWR‐R1, and LNCaP) secreted significantly higher amounts of exosomes (2‐4 folds) compared to Enz‐sensitive counterparts. Inhibition of exosome biogenesis in resistant cells by GW4869 and dimethyl amiloride strongly decreased their cell viability. Mechanistic studies revealed upregulation of syntaxin 6 as well as its increased colocalization with CD63 in Enz‐resistant PCa cells compared to Enz‐sensitive cells. Syntaxin 6 knockdown by specific small interfering RNAs in Enz‐resistant PCa cells (C4‐2B and CWR‐R1) resulted in reduced cell number and increased cell death in the presence of Enz. Furthermore, syntaxin 6 knockdown significantly reduced the exosome secretion in both Enz‐resistant C4‐2B and CWR‐R1 cells. The Cancer Genome Atlas analysis showed increased syntaxin 6 expressions associated with higher Gleason score and decreased progression‐free survival in PCa patients. Importantly, IHC analysis showed higher syntaxin 6 expression in cancer tissues from Enz‐treated patients compared to Enz naïve patients. Overall, syntaxin 6 plays an important role in the secretion of exosomes and increased survival of Enz‐resistant PCa cells.
Diffraction by a perfectly conducting wedge illuminated by a complex point source
Authors: Gentili Gian Guido , Pelosi Giuseppe , Righini Monica , Selleri Stefano 
Published: November 2019

AbstractAn elementary dipole in presence of a perfectly conducting wedge radiates a field whose analytic expression can be written in terms of a double infinite series. A dipole located at a point characterized by complex coordinates behaves in the real space as a directive source akin to a Gaussian beam in the paraxial region. In this article, the series solution, originally conceived for a dipole located at a point characterized by real coordinates, is extended to the case of a complex coordinates source, hence providing an analytical solution approximating the problem of a wedge illuminated by a Gaussian beam.
Seltenerdrecycling: Ammoniumchlorid statt Salzsäure
Authors: Lorenz Tom , Jacob‐Seifert Karin , Bertau Martin 
Published: November 2019

AbstractFeststoffchlorierung überführt universitäre Forschung in den industriellen Alltag und macht so Leuchtstoffe, Permanentmagnete und Akkus kostengünstig recycelbar. Damit würde wirtschaftliches Rezyklieren kein Widerspruch bleiben, sondern Wirklichkeit werden.
Eine neue Aufgabe für Laborgeräte
Authors: Ehrensberger Christian 
Published: November 2019

AbstractProbengeber, Spektrometer, Chromatographen, Thermostate und Datenbanken sollen untereinander so kommunizieren wie Menschen und dabei so präzise und effektiv arbeiten wie Roboter. Damit dies gelingt, müssen sich Anwender und Hersteller dafür öffnen, Daten und Know‐how in eine gemeinsame digitale Infrastruktur einzubringen.
Lothar Meyer – eine Wiederentdeckung
Authors: Kuhn Norbert , Zeller Klaus‐Peter 
Published: November 2019

AbstractDas von den Vereinten Nationen ausgerufene Internationale Jahr des Periodensystems rückt Dmitri Mendelejews Elementetafel aus dem Jahr 1869 in den Mittelpunkt. Doch es lohnt sich, einen Blick auf den anderen großen Mitbegründer des Periodensystems zu werfen: Lothar Meyer.
Stereozentrum wechsle dich
Authors: Schützenmeister Nina , de Vries Jessica 
Published: November 2019

AbstractBei der Totalsynthese von Natur‐ und Arzneistoffen ist die räumliche Orientierung der Moleküle entscheidend. Die Mitsunobu‐Reaktion hat sich bewährt, um chirale, sekundäre Alkohole gezielt in die entsprechenden Enantio‐ oder Diastereomeren zu invertieren. Eine nebenproduktarme und katalytische Reaktionsführung schien unerreichbar – bis jetzt.
Am Anfang war das Narrengold
Authors: Groß Michael 
Published: November 2019

AbstractPyrit, also FeS2, ist das häufigste Eisen‐Schwefel‐Mineral in der Erdkruste und wird in Sedimenten laufend neu gebildet. Wie, ist allerdings unklar. Zudem ist es für einige Hypothesen zu einem Urzeitstoffwechsel wichtig und stabilisiert den Sauerstoffgehalt der Atmosphäre. Dass kürzlich Mikroben entdeckt wurden, die Pyrit herstellen, hilft womöglich, alle diese Puzzlesteine zu verbinden.
International Zeolite Conference 2019
Authors: Rabe Timo 
Published: November 2019

AbstractMitte Juli fand die 19. internationale Zeolith‐Konferenz in Perth, West Australien statt. Für die sechstägige Veranstaltung hatte die International Zeolite Association (IZA) Forscher und Interessenten aus Unternehmen und Industrie eingeladen, um über Forschungsergebnisse zu porösen Materialien zu sprechen. Dabei ging es nicht nur um Zeolithe, sondern auch um poröse Silicate sowie metallorganische und kovalente organische Gerüstverbindungen. Schwerpunkt war meist die Katalyse im Zusammenhang mit der Raffination organischer Naturstoffe.
Isogeometric topology optimization of anisotropic metamaterials for controlling high‐frequency electromagnetic wave
Authors: Nishi Shinnosuke , Yamada Takayuki , Izui Kazuhiro , Nishiwaki Shinji , Terada Kenjiro 
Published: November 2019

SummaryThis study presents a level set–based topology optimization with isogeometric analysis (IGA) for controlling high‐frequency electromagnetic wave propagation in a domain with periodic microstructures (unit cells). The high‐frequency homogenization method is applied to characterize the macroscopic high‐frequency waves in periodic heterogeneous media whose wavelength is comparative to or smaller than the representative length of a unit cell. B‐spline basis functions are employed for the IGA discretization procedure to improve the performance of electromagnetic wave analysis in a unit cell and topology optimization. Also, to keep the same order of continuity on the periodic boundaries as on other element edges in the domain, we propose the extended domain approach, while incorporating Floquet periodic boundary condition (FPBC). Two types of optimization problems are taken as examples to demonstrate the effectiveness of the proposed method in comparison with the standard finite element analysis (FEA). The optimization results provide optimized topologies of unit cells qualified as anisotropic metamaterials with hyperbolic and bidirectional dispersion properties at the macroscale.
Predictors of perinatal outcome in early‐onset fetal growth restriction: A study from an emerging economy country
Authors: Baião Ana Elisa Rodrigues , de Carvalho Paulo Roberto Nassar , Moreira Maria Elisabeth Lopes , de Sá Renato Augusto Moreira , Junior Saint Clair Gomes 
Published: November 2019

What's already known about this topic?The use of fetal arterial and venous Doppler studies is well acknowledged in the context of fetal growth restriction, with ductus venosus evaluation as the most correlated with fetal acidosis and impending risk of death.What does this study add?In a subpopulation of severely growth‐restricted fetuses, the use of z‐score instead of gestational age or fetal weight alone may perform better in a prediction model including biometric and Doppler variables.
Concomitant Use of Quinolones and Stimulants and the Risk of Adverse Cardiovascular Symptoms: A Retrospective Cohort Study
Authors: Alrwisan Adel A. , Wei Yu‐Jung J. , Brumback Babette A. , Antonelli Patrick J. , Winterstein Almut G. 
Published: November 2019

ObjectiveTo examine whether concomitant use of quinolones and stimulants increases the risk of cardiac events in adults.Study DesignA retrospective cohort study of privately insured adults using MarketScan® claims data from 2008 to 2015.PatientsStimulant (methylphenidate or mixed amphetamine salts) users (18–65 yrs old) with continuous health plan enrollment for the 6 months (baseline) prior to the first dispensation (index date) of oral quinolones or comparators (amoxicillin ± clavulanate or azithromycin).Outcomes Definition(1) Cardiac symptoms (palpitation, tachycardia, or syncope); (2) cardiac arrhythmias (ventricular arrhythmias, paroxysmal ventricular tachycardia, or cardiac arrest).AnalysisBaseline covariates adjustment was through inverse probability of treatment weighting. Adults were followed until the antimicrobial therapy ended. The hazard of cardiac events in stimulant‐quinolones–exposed adults was compared to those who were treated with stimulant‐comparator antibiotics using a weighted Cox regression model. Several sensitivity analyses were performed to challenge the results robustness.ResultsThe study cohorts comprised 390,490 stimulants users who initiated either quinolone or amoxicillin, and 387,574 patients receiving stimulants who initiated quinolone or azithromycin. The unadjusted incidence rate for cardiac symptoms in stimulant‐quinolones users was 471 cases/10,000 patient‐years, and it was 244 cases/10,000 patient‐years in patients exposed to stimulant‐amoxicillin; whereas the unadjusted incidence rate for cardiac symptoms was 728 and 358 per 10,000 patient‐years for stimulant‐quinolones and stimulant‐azithromycin cohorts, respectively. Compared to stimulant‐amoxicillin use, the adjusted hazard ratio (HR) for cardiac symptoms with stimulant‐quinolones use was 1.61 (95% confidence interval [CI], 1.30–1.98). The HR for cardiac symptoms for patient exposed to stimulant‐quinolones was 1.69 (95% CI, 1.32–2.13) when compared to stimulant‐azithromycin. The sensitivity analysis findings were consistent with the primary analysis. A few patients across the study comparison groups developed cardiac arrhythmias.ConclusionConcomitant use of stimulants and quinolone was associated with an increased hazard of cardiac symptoms in comparison to concomitant use of stimulants and amoxicillin or azithromycin, but there was no apparent difference in cardiac arrhythmias.
A structural determinant of mycophenolic acid resistance in eukaryotic inosine 5′‐monophosphate dehydrogenases
Authors: Freedman Rebecca , Yu Runhan , Sarkis Alexander W. , Hedstrom Lizbeth 
Published: November 2019

AbstractMycophenolic acid (MPA) is a potent natural product inhibitor of fungal and other eukaryotic inosine 5′‐monophosphate dehydrogenases (IMPDHs) originally isolated from spoiled corn silage. MPA is produced by the filamentous fungi Penicillium brevicompactum, which contains two IMPDHs, PbIMPDHA and PbIMPDHB, both of which are MPA‐resistant. The MPA binding sites of these enzymes are identical to MPA‐sensitive IMPDHs, so the structural determinants of resistance are unknown. Here we show that a single residue, Ser267, accounts for the MPA resistance of PbIMPDHA. Substitution of Ser267 with Ala, the residue most commonly found in this position in eukaryotic IMPDHs, makes PbIMPDHA sensitive to MPA. Conversely, Aspergillus nidulans IMPDH becomes MPA‐resistant when the analogous Ala residue is substituted with Ser. These substitutions have little effect on the catalytic cycles of either enzyme, suggesting the fitness costs are negligible despite the strong conservation of Ala at this position. Intriguingly, while only 1% of fungal IMPDHs contain Ser or Thr at position 267, these residues are found in the IMPDHs from several Aspergillus species that grow at the low temperatures also favored by Penicillium. Perhaps Ser/Thr267 is an evolutionary signature of MPA exposure.