bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroprotectin D1 Protects Against Postoperative Delirium-like Behaviors in Aged Mice by Regulating Neuroinflammation Induced by Surgical Trauma
Authors: Zhou Ying , Wang Jiayu , Li Xiaofeng , Li Ke , Chen Lei , Zhang Zongze , Peng Mian 
Published: May 2020

Abstract
Background

Postoperative delirium (POD) is the most common postoperative complication affected elderly patients, yet the underlying mechanism is elusive and without effective therapy. The neuroinflammation hypothesis has been emerging as the pathogenesis of POD. Recently, accumulative evidence has supported the role of specialized proresolving lipid mediators (SPMs) in regulating inflammation. NPD1, a novel member of SPMs, is identified with potent immune-resolvent and neuroprotective effect. We aimed to investigate the role of NPD1 in neuroinflammation and postoperative cognitive function with a mice model of POD.
Methods

Aged male C57BL/6 mice were subjected to laparotomy under isoflurane anesthesia with NPD1 prophylaxis. The general and memory behaviors were assessed by buried food test, open field test and Y maze test at 6, 9, and 24 hours after the surgery. Expression level of inflammatory cytokines were analyzed by ELISA. The permeability of blood-brain barrier (BBB) was detected by spectrophotometric quantification of extravascular dextran tracer from brain tissue extracts, and the expression of tight junction (TJ) associated proteins by Western blotting. The reactive states of astrocytes and microglia were examined by immunofluorescence. The protective effects of NPD1 was also evaluated through macrophage polarization by flow cytometry, using the bone marrow-derived macrophages cultures.
Results

NPD1 prophylaxis decreased the expression of pro-inflammatory cytokines and increased the expression of anti-inflammatory cytokines in peripheral blood, hippocampus and prefrontal cortex, and limited the leakage of BBB by increasing the expression of TJ associated proteins. Besides, NPD1 prevented the activation of microglia and astrocytes both in the hippocampus and prefrontal cortex, which resulted in improved general and memory function of mice. Furthermore, NPD1 treatment modulated the inflammatory cytokine profile and promoted the macrophage polarization toward M2 in LPS-stimulated macrophages.
Conclusions

These findings verify the anti-inflammatory and proresolving activities of NPD1 in the inflammatory milieu both in vivo and in vitro, and provide better insight into the pathophysiology and potential therapy for POD.
Comparing Community Measures in Lake Microbial Ecology: Metagenomes and Metatranscriptomes and Amplicons, oh my!
Authors: Nuy Julia Kristina , Bornemann Till , Beisser Daniela , Probst Alexander J. , Boenigk Jens 
Published: May 2020

Abstract
Lake ecosystems are hotspots on Earth for biogeochemical cycling yet linking their microbiome to physicochemical parameters remains a challenge. Here, we assess the quality of 16S rRNA gene-based metatranscriptomics, assembled metagenomics and 16S rRNA gene amplicon sequencing for 21 lake ecosystems across Europe. We identified method-dependent, massive differences between community composition and proportional activity for key taxa like Alphaproteobacteria suggesting different ecological conclusions for the same lake ecosystems. In redundancy analysis (RDA), environmental parameters explained the greatest amount of the variance in metatranscriptomes suggesting that the active community is heavily influenced by environmental parameters. While amplicon data recruited the least amount of environmental variables in RDA (pH and temperature), four additional parameters explained the sequenced metagenomes. These results suggest that metagenomes and metatranscriptomes are currently the best methods for linking lake microbiomes to physicochemical parameters and can be used as proxies for designing future ecological surveys.
Development and Validation of Oral Chemotherapy Self-Management Scale
Authors: Peng Qi , Wu Wanying 
Published: May 2020

Abstract
Background With the widespread used of orally administered anticancer agents, the self-management is important for patients with oral chemotherapy, because it’s occurs in the home environment, not clinic settings.Methods OCSMS items were developed through literature review, semi-structured interviews, research seminar and Delphi survey. Their content validity, internal consistency, split-half, test-retest reliability and construct validity were assessed.Results A 36-item scale was developed with five dimensions identified through factor analysis: daily life management, symptom management, medication management, emotional cognitive managemen and social support. Cronbach’s coefficient Alpha, split-half coefficient, test-retest reliability and average content validity index (CVI) scores were 0.929, 0.773, 0.966 and 0.833, respectively, indicating that OCSMS has good reliability and validity.Conclusions The OCSMS is a valid, reliable measurement method of the self-management ability of patients with oral chemotherapy. The OCSMS shows potential as a tool to ensure the safety of patients with cancer. The OCSMS may help evaluate the effectiveness of interventions to improve the self-management ability of patients.
RasV12 Expression in Microglia Promotes Retinal Inflammatory Circuits and Impairment of Photoreceptor
Authors: Moriuchi Yuta , Iwagawa Toshiro , Tsuhako Asano , Koso Hideto , Fujita Yasuyuki , Watanabe Sumiko 
Published: May 2020

Abstract
Background Microglial activation is commonly observed in neurodegenerative diseases. However, its role in the complex inflammatory network remains unclear. In this study, we generated a microglial activation mouse model by inducing the expression of a constitutively active form of Ras in microglia.Methods The double transgenic lines CAG-LSL-RasV12-IRES-EGFP; Cx3cr1CreER (Cx3cr1-RasV12 mice) and CAG-LSL-EGFP;Cx3cr1CreER (control mice) were generated. The expression of RasV12 was induced in microglia by tamoxifen administration. Effects of the expression of RasV12 in the retina were examined by immunohistochemistry of frozen sections, RT-qPCR, and live imaging.Results RasV12 expression in retinal microglial cells promoted cell proliferation, cytokine expression, and phagocytosis. RasV12-expressing microglia migrated towards the apical and basal regions of the retina. Examination of GFAP expression revealed activation of Müller glia in the retina. We also observed loss of the photoreceptors in the outer nuclear layer (ONL) in close proximity to microglial cells. However, no neurodegeneration was observed in the inner nuclear layer (INL) or ganglion cell layer (GCL). Furthermore, we found that RasV12-expressing microglia in the ONL were smaller in size and engulfed photoreceptors. In contrast, the morphology of RasV12-expressing microglia in the GCL and INL resembled resting microglia. In conclusion, RasV12-induced microglial activation impaired rod photoreceptor survival in the ONL, but not in other regions of the retina.Conclusion The expression of RasV12 is sufficient to activate microglia, and our results suggested that microenvironment cues may modulate the microglial phenotypic features and effects of microglial activation.
Lateral Access Spine Surgery Following Liver Transplantation: A Report of Two Cases
Authors: Akeda Koji , Takegami Norihiko , Yamada Junichi , Kishiwada Masashi , Sakurai Hiroyuki , Mizuno Shugo , Isaji Shuji , Sudo Akihiro 
Published: May 2020

Abstract
Background With improving prognosis for recipients of liver transplantation, the necessity of medical care for musculoskeletal disorders, including spinal diseases, of these patients is also increasing. Only few reports are available on cases of spine surgery following liver transplantation. Furthermore, a case of lateral access surgery following liver transplantation has not thus far been reported. The purpose of this study is to present first two cases of patients treated with lateral access spine surgery following liver transplantation.Case presentation Case 1. A 49-year-old female had received living donor liver transplantation (LT) for acute-on-chronic liver failure (ACLF) ten years prior to spine surgery. The patient underwent two levels of lateral lumbar interbody fusion (LLIF) followed by posterior lumbar interbody fusion (PLIF) for degenerative lumbar disease. Although neurological symptoms in the lower extremity improved, a liver disorder resulting from acetaminophen-induced hepatotoxicity occurred at an early stage following spine surgery.Case 2. A 66-year-old female had received living donor liver transplantation for hepatocellular carcinoma (HCC) six years prior to spine surgery. She underwent posterior instrumentation surgery followed by T12 corpectomy using a wide-foot print expandable cage for T12 vertebral collapse. Following surgery, her weakened muscle strength in the lower extremities were significantly improved. Conclusions Lateral access spine surgery for lumbar and thoracolumbar lesions can be successfully performed for patients following liver transplantation. However, careful follow-up should be performed for complications related to the function of the liver graft following spine surgery.Conclusions Lateral access spine surgery for lumbar and thoracolumbar lesions can be successfully performed for patients following liver transplantation. However, careful follow-up should be performed for complications related to the function of the liver graft following spine surgery.
Prognostic value of members of the HSP70 family in Hepatocellular Carcinoma
Authors: Jiang Haixing , Liang Ziyu , Qin Shanyu , Liu Kang 
Published: May 2020

Abstract
BACKGROUND: Despite multiple functions in the disease, the prognosis of the heat shock protein (HSP) 70 family in Hepatocellular Carcinoma (HCC) remains unclear. METHODS: The UALCAN database has provided information about the expression level of HSP70 family members in both HCC and in normal tissues. Overall survival (OS) was conducted by Kaplan-Meier plotter (KM plotter). Gene ontology (GO) and KEGG pathway enrichment analyses were accomplished by DAVID database. GeneMANIA and STRING were applied to construct gene-gene and protein-protein interaction (PPI) networks. RESULTS: From the UALCAN database, we found that the expression levels of eight members of the HSP70 family in HCC patients were higher than in normal liver tissues. These eight members were, namely, HSPA1A, HSPA1B, HSPA1L, HSPA2, HSPA5, HSPA6, HSPA8 and HSPA9. From KM plotter database, high expression of HSPA1A, HSPA1B, HSPA6 and HSPA8 has been observed to be associated with worse OS in patients with HCC (hazard ratio [HR] =1.49, 95% confidence interval [CI]: 1.03-2.15, P=0.031; HR=1.49, 95%CI: 1.05-2.12, P=0.026; HR=1.53, 95%CI: 1.06-2.2, P=0.021 and HR=1.81, 95%CI: 1.21-2.71, P=0.0036, respectively). We also found that the high expressions of HSPA1A, HSPA1B, HSPA6 and HSPA8 were related to unfavorable OS of HCC patients with other factors such as clinical stage, gender, race and hepatitis virus. From GO and KEEG analysis, we detected a close relationship between these eight genes and Biological process, Cellular component, Molecular function and cancer related signaling pathways. The PPI network and GeneMANIA results showed that there was a strong co-expression relationship between the protein homology and the genes. CONCLUSIONS: According to previous studies and analysis, when HSPA1A, HSPA1B, HSPA6 and HSPA8 were highly expressed in HCC patients, they generally demonstrated lower OS. Therefore, the members of HSP70 family are likely to be used as drug targets and prognostic biomarkers when treating HCC patients.
Gender Barriers to Knowledge Transfer and Exchange Among Vaccine Researchers in Low-, Middle- and High-Income Countries – An International Cross-Sectional Study in 44 Countries
Authors: El-Halabi Soha , McCabe Ronan , Forsberg Birger C. , Elling Devy L , El-Khatib Ziad 
Published: May 2020

Abstract
Background: Globally, women constitute 30% of researchers. Despite an increasing proportion of women in research, they are still less likely to have international collaborations. Literature on barriers to knowledge transfer and exchange (KTE) between men and women, remain limited. This study aimed to elucidate perceived gender barriers to KTE activities in vaccination related research in low, middle- and high-income countries. Methods: This was a cross-sectional data from a self-administered questionnaire distributed to researchers in the field of vaccination research. Structural factors to KTE were assessed using 12 statements measured with 5-point Likert scale, ranging from 1 (strongly disagree) to 5 (strongly agree). An index ranging from 12 to 60 points was created to assess structural factors to KTE, with higher score indicating higher perceived barriers. Linear regression modelling was applied to examine the association between KTE barriers and gender. Results: Regardless of gender, researchers experienced challenges with respect to KTE activities, particularly factors related to the availability of human and financial resources, and level of technical expertise among their target audience. We were also able to identify perceived facilitators among men and women, such as the presence of structures that link researchers and target audiences, the investment of target audiences in KTE efforts and the presence of stable contacts among target audience. Our linear regression analysis showed that women have a reduction of 1.069 in their scores in comparison with men (R2=0.014; B=-1.069; 95%CI -4.035;1.897). Conclusions: Men and women shared common perspectives on barriers to KTE. KTE activities could be strengthened by improving structural efforts to reduce gender differences and increase collaborations between researchers and their target audience.
Individualized mechanical ventilation in a shared ventilator setting: limits, safety and technical details.
Authors: Stiers Michiel , Mergeay Matthias , Pinson Hannah , Janssen Luc , Voets Evy , De Cauwer Harald , Schepens Tom 
Published: May 2020

Abstract
Background- The COVID-19 pandemic has resulted in an increased need for ventilators. The potential to ventilate more than one patient with a single ventilator, a so-called split ventilator setup, provides an emergency solution. Our hypothesis is that ventilation can be individualized by adding a flow restrictor to limit tidal volumes, add PEEP, titrate FiO 2 and monitor ventilation. This way we could ensure optimization of patient safety and clinical applicability. We performed bench testing to test our hypothesis and identify limitations. Methods- We performed a bench testing in two lungs: 1) determine lung compliance 2) determine volume, plateau pressure and PEEP, 3) illustrate individualization of airway pressures and tidal volume with a flow restrictor, 4a) illustrate that PEEP can be applied and individualized 4b) create and measure intrinsic PEEP 4c-d) determine PEEP as a function of flow restriction, 5) individualization of FiO 2 . Results- The lung compliance varied between 13 and 27 mL/cmH 2 O. Set ventilator settings could be applied and measured. Extrinsic PEEP can be applied except for settings with a large expiratory time. Volume and pressure regulation is possible between 70-39% flow restrictor valve closure. Flow restriction in the tested circuit had no effect on the other circuit or on intrinsic PEEP. FiO 2 could be modulated individually between 0.21 and 0.8 by gradually adjusting the additional flow, and minimal affecting FiO 2 in the other circuit. Conclusions- Tidal volumes, PEEP and FiO2 can be individualized and monitored in a bench testing of a split ventilator. In vivo research is needed to further explore the clinical limitations and outcomes, making implementation possible as a last resort ventilation strategy.
A sertéshússal és a sertésből készült termékekkel kapcsolatos preferenciák vizsgálata egyetemista hallgatók körében
Authors: Nagy Orsolya Bernadett 
Published: May 2020

A tanulmány célja a sertéshússal és a sertésből készült termékekkel kapcsolatos fogyasztói preferenciák vizsgálata volt. Az elemzéshez felhasznált minta (N=324) a Debreceni Egyetem Gazdaságtudományi Karának hallgatói köréből került kiválasztásra. A felmérés online kérdőíves formában zajlott le. Az adatok feldolgozásához leíró statisztikák, Khi-négyzet próbák, Spearman-féle rangkorreláció vizsgálatok, Mann-Whitney és Kruskal-Wallis próbák használata valósult meg. Az eredmények alapján elmondható, hogy a vizsgált mintában szereplő fogyasztók többsége, mindössze 50% alatti összeggel lenne hajlandó többet fizetni a magyar és az egészségesebb termékekért. Az elemzések során szignifikáns eltérés született a férfiak és nők között minden vizsgálatba bevont a vásárlásnál figyelembe vett tényező tekintetében; a középfokú és a felsőfokú végzettséggel rendelkezők között a gyártó/márka megítélésében; a jövedelemkategória csoportoknál pedig az ár és az egészséges táplálkozás szerint.
An exploratory study on the level of trust among Hungarian food manufacturer companies
Authors: Erdei Edina 
Published: May 2020

The intensification of globalized economic competition is playing an increasingly important role in the lives of companies to determine their true position among their competitors. Food companies are of paramount importance because of their role and weight in the national economy. In Hungary, the food industry plays an important social and economic role and is a world leader in terms of employment and value added. Currently, there are nearly 1100 active food companies in Hungary, so it is worth examining the peculiarities of the role of trust between companies.
There are often contradictory statements in the literature about the effects of Industry 4.0 technologies and the trust placed in IT tools. Therefore, it is a relevant research question to examine whether the characteristics of the role of trust - e.g. staff, suppliers, IT tools and technologies, etc. as confidence levels can have a direct positive impact on the efficiency, profits, liquidity, etc. of the companies in question. development.
During the research, I analyzed the level of trust between the food trading companies, in which I discuss the trust within the industry within the company. My aim is to further enrich my previous research knowledge in this field.
Az intellektuális tőke hatékonysága, mint meghatározó innovációs tényező
Authors: Béresné Mártha Bernadett , Lakatos Vilmos , Tömöri Gergő 
Published: May 2020

Napjaink világgazdaságában erőteljes elmozdulás érzékelhető a termelő vállalatok felől a szolgáltatások irányába, amely mind a gazdaságpolitikai döntéshozókat, mind az üzleti vállalkozás érintettjeit, a tulajdonosokat, beszállítókat, versenytársakat, illetve a hétköznapi embereket, a fogyasztókat is kihívás elé állítja: a szerkezetváltás egyaránt ösztönöz minden gazdasági szereplőt arra, hogy megtanuljon alkalmazkodni az új tendenciákhoz. Kutatásunk fókuszában éppen ezért az innovációt és a vállalatok értékteremtő képességét állítottuk. Az IT szektor jelenleg az egyik leggyorsabban fejlődő és leginnovatívabb ágazatnak számít a szolgáltató iparágon belül. Az IT vállalatok sikerességét jelentős mértékben meghatározza az a képessége, hogy mennyire hatékonyan használják fel a rendelkezésükre álló immateriális javakat, azon belül is az intellektuális tőkét, amelyet viszont nem pénzügyi értékteremtő eszközként definiálunk. Felmerülhet tehát a kérdés: hogyan lehetséges számszerűsíteni, pénzben kifejezni a vizsgálni kívánt vállalatok intellektuális tőkéjét? A legsikeresebb magyar IT vállalatokra vonatkozóan éppen ezért kiszámítottuk a széles körben elismert mutatót, a VAICTM mutatót, melyre vonatkozóan hazai számítások még nem állnak rendelkezsére. Ez a mutató kiküszöböli a beszámolási sztenderdekből fakadó információhiányt azzal, hogy a módszertan a mindenki által hozzáférhető, nyilvános pénzügyi beszámolók adatain alapul. Maga a mutató egy csúcsmutató, amelynek két fő részmutatója az intellektuális tőke hatékonyságát illetve a fizikai tőke hatékonyságát számszerűsíti.
Supplier Evaluation Schemes in Case of Different Research Frameworks
Authors: Pató Gáborné Szűcs Beáta , Kiss Fanni 
Published: May 2020

Performance evaluation is not only important in employer and employee relationships, but also in partnerships between a supply chain’s members. In order to satisfy customers’ needs according to the 7 Rights of logistics, all members of the given chain have to perform properly. To ensure the best performance, supplier evaluation can act as a starting point. Besides continous feedback, supplier evaluation plays an important role in performance development of suppliers. The aim of our research is to develop and introduce innovative supplier evaluation models, which include the advantages of traditional and electronic supplier evaluation forms. In addition to this, these models are visualised, making it easier to understand expectations and information. In this study, the aim is to present two different models the PaTeNt©- SESC (Pató Tetrahedrons of interNational Theory - Supplier Evaluation of Supply Chain) and the Douple Evaluation Platform, based on previous research.
The Slow Road to the Social Investment Perspective in the European Union
Authors: Dinan Shannon 
Published: May 2020

The European Union has no unilateral legislative capacity in the area of social policy. However, the European Commission does play the role of guide by providing a discursive framework and targets for its 28 Member States to meet. Since the late 1990’s, the EU’s ideas on social policy have moved away from the traditional social protection model towards promoting social inclusion, labour activation and investing in children. These new policies represent the social investment perspective, which advocates preparing the population for a knowledge-based economy to increase economic growth and job creation and to break the intergenerational transmission of poverty. The EU began the gradual incorporation of the social investment perspective to its social dimension with the adoption of ten-year strategies. Since 2000, it has continued to set goals and benchmarks as well as offer a forum for Member States to coordinate their social initiatives. Drawing on a series of interviews conducted during a research experience in Brussels as well as official documents, this paper is a descriptive analysis of the recent modifications to the EU’s social dimension. It focuses on the changes created by the Europe 2020 Strategy and the Social Investment Package. By tracing the genesis and evolution of these initiatives, the author identifies four obstacles to social investment in the European Union's social dimension.

Full text available at: https://doi.org/10.22215/rera.v10i1.263
The European Stability Mechanism and the IMF: From the Enhanced Cooperation to Embedded Supervisor
Authors: Climie Cameron 
Published: May 2020

This paper examines the role played by the International Monetary Fund (IMF) in the European Stability Mechanism (ESM), finding that the ESM has greatly expanded the IMF’s surveillance and oversight roles in the European Monetary Union (EMU). Building from a liberal intergovernmental framework of integration analysis, this paper argues that the IMF’s function as a de facto EMU supervisor in the ESM, a significant break from prior European integration, stems from the alignment of crisis response preferences amongst the EMU’s largest economies, the erosion of the credibility of the European Commission as an enforcer of structural reforms, and the IMF’s close fit with the preferred institutional arrangements that derived from the bargaining dynamics between euro members.

Full text available at: https://doi.org/10.22215/rera.v12i1.1232