bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcome of Free-Flap Transfer for Head and Neck Reconstruction in Hypercoagulable Patients
Authors: Zhou Wei , Zhang Wen-Bo , Yu Yao , Wang Yang , Mao Chi , Guo Chuan-Bin , Yu Guang-Yan , Peng Xin 
Published: June 2020

Abstract
Background: The purpose of this study was to examine the outcome and complications of hypercoagulable patients undergoing free-flap transfer in the head and neck region. Methods：We retrospectively reviewed the data of 685 consecutive free-flap transfers in the head and neck region performed by a single surgical team at the Peking University School and Hospital of Stomatology between January 2013 and December 2018. Based on preoperative coagulation indices the patients were separated into two groups: those with hypercoagulablity (group A, n = 45) and those with normal coagulation indices (group B, n = 640). Demographic characteristics, thrombosis and flap failure were compared between the two groups. Chi-square test and repeated-measures ANOVA were used for data comparisons.Results：Microvascular thrombosis rate (P = 0.42) and free-flap success rate (P = 0.38) were not significantly different between the groups. The platelet count and activated partial thromboplastin time changed significantly during the perioperative period in the hypercoagulable group (repeated-measures ANOVA, P < 0.001 for both).Conclusions：Hypercoagulability does not seem to increase risk of free-flap failure in head and neck microsurgery provided standard anticoagulation protocols are followed.
Peritoneal Serous Borderline Tumour with Ultra-Mutated NGS Profile Including POLE E, BRAF, RB1, HER2 and P53 Mutations : A Case Report.
Authors: Noel Jean-Christophe , Buonomo Antonio , Bouri Sarah , D’Haene Nicky , Catteau Xavier 
Published: June 2020

Abstract
Background: Serous borderline tumors of the peritoneum are rare low-grade epithelial proliferation with a tubal-type differentiation occurring in the pelvis or abdomen without underlying tissue invasion that resemble to ovarian serous borderline tumors but by definition without ovrian or tubal involvement. To date, the mutation status in molecular biology of these tumours remains largely unknown.Case presentation: Here we describe the case of a borderline peritoneal serous tumour which occurred in a 48 year old patient in whom an analysis of the mutation status by NGS technique identified a ultra-mutated profile including POLE E, BRAF, RB1, HER2 and p53 mutations.Conclusions: To the best of our knowledge, this particular mutation status has never been described in this type of tumour. It could represent an early event with transient mutations, most of them will not fixate on the genome.
Osteogenic effect in rat osteoporosis model and femur defect model by intraosseous administration of simvastatin microcrystals
Authors: Zhu Junxiong , Zhang Chenggui , Jia Jialin , Wang Hong , Leng Huijie , Xu Yingsheng , Wu Cuishuan , Zhang Qiang , Song Chunli 
Published: June 2020

Abstract
Background: Currently, drugs for local bone formation are very limited in clinic. Simvastatin is one of most potential translational drugs by repositioning for its significant osteogenic effect and its history as a safe lipid-lowering medicine. In this study, we prepared the pre-clinical simvastatin microcrystals which could be potentially translated and industrialized. Methods: Simvastatin microcrystals were made by wet media milling method. Characterization of microcrystal particles were evaluated by laser particle size analyzer and high-performance liquid chromatography (HPLC). Furthermore, we verified the osteogenic effect in rat ovariectomy(OVX)-induced osteoporosis model and femur defect model. For osteoporosis model, we delivered simvastatin microcrystals to tibia with poloxamer hydrogel by intraosseous injection. Bone mineral density (BMD) and ultimate force were assessed after treatment. For femur defect model, simvastatin microcrystal was incorporated in clinically used calcium phosphate cements (CPCs) as an implant. Histology and μCT were used for evaluation of the healing. Results: The D 10 , D 50, and D 90 showed size of the most particles ranged from 0.226-3.425 μm. The mean particle size of the microcrystals is determined as 1.365 μm which demonstrated a successful preparation. For osteoporosis model, BMD and the ultimate force of the treated tibia were significantly improved in osteoporosis rat after injection of 0.5 mg and 1 mg simvastatin microcrystals compared with OVX or 0 mg groups. There were no differences observed in BMD and ultimate force between 0.5 mg, 1 mg simvastatin microcrystals group and bone morphogenetic protein (BMP) 5 ug group. For femur defect model, quantitative analysis of bone regeneration by μCT showed bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N) were significantly increased in simvastatin microcrystals 50ug (SIM-C 50 ug), SIM-C 100 ug and BMP 5 ug group compared with CPC vehicle group. Trabecular separation (Tb.Sp), and cement volume/tissue volume (CV/TV) were decreased in SIM-C 50 ug, SIM-C 100 ug and BMP 5 ug groups compared with CPC vehicle group. Semiquantitative scale for histology assessment further demonstrated a higher bone regeneration score in drug loaded groups compared with CPC group.﻿Conclusions: Our study shows that simvastatin microcrystals were successfully prepared by wet media milling method and the microcrystals can promote bone formation by local delivery by poloxamer hydrogel or CPC, which is of great translational potential.
Prognostic Value of Quantitative 18F-FDG PET/CT Parameters Measured in Primary Tumors and Suspicious Lymph Nodes in Patients With Esophageal Carcinoma
Authors: Tustumi Francisco , Takeda Flávio Roberto , Duarte Paulo Schiavom , Albenda David Gutiérrez , Sallum Rubens Antonio Aissar , Junior Ulysses Ribeiro , Buchpiguel Carlos Alberto , Cecconello Ivan 
Published: June 2020

Abstract
Objective:Quantitative 18F-FDG PET/CT parameters have been described as prognostic indicators in esophageal cancer. The objective of this study isto evaluate the prognostic value of the maximum standardized uptake value (SUVmax), metabolic tumor value (MTV) and total lesion glycolysis (TLG) measured in the primary tumor and suspicious lymph nodes.Methods: A cohort study was performed to assess the association of SUVmax, MTV and TLG measured prior to and post neoadjuvant therapywithoverall survival (OS) of patients with esophageal cancer who received trimodal therapy. The quantitative techniques were applied in the primary tumor and suspicious lymph nodes. The OS rates were analyzed. Results: Before neoadjuvant therapy, 106 patients underwent PET/CT, and 39 patients underwent post-neoadjuvant therapy PET/CT exams. Before neoadjuvanttherapy, PET/CT showed that all the variables of the evaluated lymph nodes were statistically significant in predicting OS. Postneoadjuvanttherapy, none of the PET/CT variables of lymph nodes were related to prognosis. On the other hand, all primary tumor volumetric variables were related to overall survival. The MTV (HR: 4.66; 95% CI: 1.54-14.08) and TLG (HR: 4.86; 95% CI: 1.66-14.26) of the primary tumor post neoadjuvanttherapy and the variations in MTV (HR: 2.95; 95% CI: 1.01-3.52) and TLG (HR: 3.49; 95% CI: 1.01-3.52) of the primary tumor pre-to-post-neoadjuvanttherapy were prognostic variables. Conclusion: ﻿In patients with esophageal cancer, the burden of disease in suspicious lymph nodes and the primary tumor prior to therapy and the residual burden of disease in the primary tumor post therapy assessed by PET/CT were associated with prognosis.
Comparison of Beta Diversity Measures in Clustering the High-dimensional Microbial Data
Authors: Chen Biyuan , He Xueyi , Pan Bangquan , Zou Xiaobing , You Na 
Published: June 2020

Abstract
Background: Heterogeneity of disease is a major concern in medical research, which is commonly recognized as subtypes with different pathogenesis, exhibiting distinct prognosis and treatment effects. The classification of population into homogenous subgroups is challenging, especially for the complex diseases. Recent studies show that gut microbiome compositions play a vital role in disease development, and it is of great interest to cluster the patients according to their microbial profiles. There are a variety of beta diversity measures to quantify the dissimilarity between the compositions of different samples for clustering. However, using different beta diversity measures results in different clusters, and it is difficult to make a choice.

Results: Considering the microbial compositions from 16S rRNA sequencing, which are presented as a high-dimensional vector with large proportion of extremely small or even zero-valued elements, we set up three simulation experiments to mimic the microbial compositional data and evaluate the performance of different beta diversity measures in clustering. Their performance in two real datasets demonstrates the validity of the simulation experiments.

Conclusion: It is shown that J-divergence/Jensen-Shannon divergence/square root of Jensen-Shannon divergence and Bhattacharyya/Hellinger can capture the compositional changes at low abundance elements more efficiently, and Bhattacharyya/Hellinger is suggested.
Barriers and Facilitators for Implementation of a Patient Prioritization Tool in Rehabilitation Settings
Authors: Déry Julien , Ruiz Angel , Routhier François , Bélanger Valérie , Côté André , Ait-Kadi Daoud , Gagnon Marie-Pierre , Lamontagne Marie-Eve 
Published: June 2020

Abstract
Introduction. Prioritization tools aim to manage access to care by ranking patients equitably in waiting list based on determined criteria. Patient prioritization has been studied in a wide variety of clinical health services, including rehabilitation contexts. We created a web-based patient prioritization tool with the participation of stakeholders in two rehabilitation programs, which we aim to implement into clinical practice. Successful implementation of such innovation can be influenced by a variety of determinants. The goal of this study was to explore facilitators and barriers to the implementation of a patient prioritization tool in rehabilitation programs.Methods. We used two questionnaires and conducted two focus groups among service providers from two rehabilitation programs. We used descriptive statistics to report results of the questionnaires and qualitative content analysis based on Consolidated Framework for Implementation Research.Results. Key facilitators are the flexibility and relative advantage of the tool to improve clinical practices and produce beneficial outcomes on patients. Main barriers are the lack of training, financial support and human resources to sustain the implementation process.Conclusion. This is the first study that highlights organizational, individual and innovation levels facilitators and barriers for the implementation of a prioritization tool from service providers’ perspective.Contributions to the literatureMethods used in this study could be operationalized in future studies to investigate barriers and facilitators of the implementation of an innovative intervention in rehabilitation settings.We used a well-known implementation framework (CFIR) to classified the determinants of the implementation, which could help to compare the results with other similar studies in implementation science.The barriers and facilitators identified in this study are an important first step in the implementation process of a patient prioritization tool in rehabilitation programs.
Effectiveness of Scoliosis-Specific Exercises for Alleviating Adolescent Idiopathic Scoliosis: A Systematic Review
Authors: Fan Yunli , Ren Qing , TO Michael KT , Cheung Jason Pui Yin 
Published: June 2020

Abstract
Background
Adolescent idiopathic scoliosis (AIS) is the most common pediatric spinal deformity with reported complications including pain, mental health concern and respiratory dysfunction. The Scoliosis-specific exercise (SSE) is prescribed throughout pubertal growth to slow progression although effects are unclear. This review aims to establish the effectiveness of SSE in alleviating AIS in terms of reducing Cobb angle, improving trunk asymmetry and quality of life (QoL). Additionally, it aims to define the effects of age, skeletal maturity, curve magnitude and exercise compliance on the outcomes of SSE.
Methods
A systematic reviewed was conducted to net SSE articles. Searched databases included PubMed, MEDLINE, Cochrane Library, Scopus, CINAHL and Google scholar. The quality of study was critically appraised according to the PEDro scale.
Results
A total of ten trials with an average PEDro score of 6.9/10 were examined in this study. Two randomized controlled trials (RCTs) and two clinical controlled trials suggested that SSE alone and with bracing or traditional exercise had clinical significance in reducing Cobb angle more than 5°. One RCT specifically implicated no comparable effects between bracing and SSE in prevention of curve progression for moderate scoliosis. Insufficient evidence demonstrated the positive effects of SSE in improving truck asymmetry (n = 4) and QoL (n = 3). Five studies evaluated the interaction effects of age (n = 2), skeletal maturity (n = 1) and curve magnitude (n = 2) with SSE in reducing Cobb angle yet without drawing any firm conclusions.
Conclusions
Insufficient evidence is available to prove that SSE with or without other conservative treatments can reduce Cobb angle, improve trunk balance and QoL. The interaction effects of age, skeletal maturity, curve magnitude, and exercise compliance with SSE in reducing Cobb angle are not proven. Future studies should investigate the relationship of influencing factors and SSE in treating AIS but not only testing its effectiveness.
Effect of eight weeks of cervical stability exercise on flexion relaxation phenomenon, pain, and disability in patients with non-specific chronic neck pain
Authors: Shamsi Hasan , Khademi-Kalantari Khosro , Akbarzade-Baghban Alireza , Izadi Neda , Okhovatian Farshad 
Published: June 2020

Abstract
Background: The effect of therapeutic exercise on cervical flexion relaxation phenomenon (FRP) is questionable. This study aimed at investigating the effect of eight weeks of cervical stability exercise on FRP, pain, and disability in NSCNP patients.Methods: This quasi-experimental study was conducted on 25 subjects with NSCNP. Surface electromyographic signals of the cervical erector spinae (CES) muscles in a sitting position were recorded before and after the intervention. The occurrence of FRP and flexion relaxation ratio (FRR) were analyzed before and after the eight weeks of cervical stability exercise. The low-load craniocervical flexion exercise was performed by the subjects in crook lying position. In addition, the intensity of pain and disability were assessed via visual analogue index (VAS) and neck disability index (NDI).Results: The FRR of CES muscles increased after the intervention. Similarly, the occurrence of FRP increased among NSCNP patients after the intervention. The average changes of VAS, NDI, and FRR were not associated with gender and duration of pain. Moreover, VAS and NDI scores significantly decreased after the intervention. Conclusions: Flexion relaxation phenomenon can be improved via specific stability exercises. The improvement of flexion relaxation phenomenon apparently can pain and disability.
Capillary blood reference intervals for platelet parameters in healthy full-term neonates in China
Authors: Cui Dongyan , Hou Yan , Feng Ling , Li Guo , Zhang Chi , Huang Yanli , Fan Jiubo , Hu Qun 
Published: June 2020

Abstract
Background
No consensus has been reached on reference intervals for platelet parameters in full-term neonates. We aimed to establish neonatal capillary blood reference intervals for platelet parameters and evaluate influences of gender, gestational age and postnatal age on platelet parameters.
Methods
This study was implemented in 594 healthy full-term neonates from 12 to 84 hours of age, using SYSMEX XN-9000 haematology automatic analyser by means of capillary blood. Reference intervals for platelet parameters were defined by an interval of 2.5th − 97.5th percentiles.
Results
Capillary reference interval for platelet count was (152–464) × 109/L. No significance was found between gender-divided reference intervals for platelet parameters. The values of platelet count changed significantly across gestational age and postnatal age. Reference intervals for other platelet parameters were affected by these factors to a different extent.
Conclusions
We established capillary blood reference intervals for platelet parameters in the early phases after birth of full-term neonates in China for the first time.
Factors associated with minimum dietary diversity and meal frequency among children aged 6-59 months in northwest Ethiopia: finding from the baseline survey of nutrition project
Authors: Bikes Gashaw Andargie , Tariku Amare , Wassie Molla Mesele , Mekonnen Solomon , Muhammad Esmael Ali , Belew Aysheshim Kassahun , Abebe Zegeye , Gete Azeb Atnafu , Yesuf Melkie Edris , Awoke Tadesse , Fekadu Abel , Gebeye Ejigu , Kebede Yigzaw , Alemu Kassahun , Addis Abebaw , Fentahun Kindie , Sisay Mekonnen , Gonete Kedir Abdela , Hunegnaw Melkamu Tamir 
Published: June 2020

Abstract
Background: Studies on the feeding practice of children, including meal frequency and dietary diversity are scarce among children. Therefore, this study aimed to assess dietary diversity and meal frequency and associated factors among children aged 6-59 months at Dabat Health and Demographic Surveillance System (HDSS) site, northwest Ethiopia.Methods: A community based cross-sectional study was conducted at Dabat HDSS site from February to June 2016 on 1,174 mother-child pairs. A multistage stratified sampling followed by a systematic random sampling technique was employed to select the study participants. A binary logistic regression model was fitted to identify factors associated with dietary diversity and meal frequency. Variables having a p-value < 0.2 in the bivariate logistic regression were entered into multivariate logistic regression and a p-value < 0.05 were considered statistically significant with feeding practices.Result: The overall prevalence of a minimum dietary diversity practice and a minimum meal frequency was 27 % (95%CI: 24.4, 29.7) and 83.7 % (95%CI: 86.0, 91.7), respectively. Having antenatal care (ANC) service (AOR = 1.6; 95% CI: 1.18, 2.27) and institutional delivery (AOR = 2.6; 95% CI: 1.85, 3.55) increased odds of adequate dietary diversity. On the contrary, the odds of practicing an adequate dietary diversity was lower among a household obtained food from home gardens (AOR = 0.4; 95% CI: 0.29, 0.52) and currently breast fed children (AOR = 0.6; 95% CI: 0.42, 0.78). The probability of having a minimum meal frequency was decreased among children in the first two years of age (AOR = 0.1; 95% CI: 0.06, 0.15), however increased in children who fed alone (AOR = 1.9; 95% CI: 1.29, 2.69). Conclusion: The proportion of children who received the minimum dietary diversity was low while the proportion of children who obtained minimum meal frequency was good. Providing a health and nutrition counseling on Infant and Young Child Feeding (IYCF) during maternal ANC services and delivery period are recommended for achieving the recommended dietary practices.
The present shock and time re-appropriation: rethinking science education in the pandemic era
Authors: Levrini Olivia , Fantini Paola , Barelli Eleonora , Branchetti Laura , Satanassi Sara , Tasquier Giulia 
Published: June 2020

Abstract
The crisis due to the COVID-19 pandemic is so huge that raises enormous questions about its implications for our societies and personal lives. In this article, we focus on science education and the role it can play to prepare future generations to grapple with this complex, fast-changing and fragile society. The approach we chose is centered on the concept of time, whose perception and organisation has been deeply affected by pandemic-induced changes. An exploratory qualitative study has been carried out with secondary school students to investigate how they were experiencing time during lockdown and which (if any) scientific knowledge they were activating to grapple with the current uncertainty and change in time structures.The main result concerns the discovery of the potential of the dichotomy between alienation from time and time re-appropriation to highlight the difference between future shock, (characteristic of the pre-crisis society of acceleration) and present shock (characteristic of the pandemic era). The analysis of students’ interviews allowed us to start unpacking time re-appropriation and its links with science learning and identity development.On the basis of the analysis, we identified three directions that science education research could explore to support schools and science teaching in playing a leading role in this fast-changing society. The directions concern new thematic and epistemological priorities for science curricula, new emphasis on learning and teaching rituals fostering appropriation, and arguments for revising the institutional role of schools.
Circular RNA SIPA1L1 Promotes Osteogenesis via Regulating the MIR-617/SMAD3 Axis in Dental Pulp Stem Cells
Authors: Ge Xingyun , Li Zehan , Zhou Zhou , Xia Yibo , Bian Minxia , Yu Jinhua 
Published: June 2020

Abstract
Background: Bone regeneration is preferred for bone loss caused by tumors, bone defects, fractures, etc. Recently, mesenchymal stem cells are considered as optimistic tools for bone defect therapy. Dental pulp stem cells (DPSCs) are a promising candidate for regenerative medicine and bone regeneration. Our previous study showed that up-regulated circSIPA1L1 during osteogenesis of DPSCs is of significance. In this paper, the potential role of circSIPA1L1 in osteogenesis of DPSCs and its underlying mechanisms are explored.Methods: The circular structure of circSIPA1L1 was identified by Sanger sequencing and PCR. Regulatory effects of circSIPA1L1 and miR-617 on mineral deposition in DPSCs were assessed by alkaline phosphatase (ALP) and alizarin red S (ARS) staining and in vivo bone formation assay were conducted to verify the biological inﬂuences of circSIPA1L1 on DPSCs. Western blot was performed to detect the protein expression of Smad3. Localization of circSIPA1L1 and miR-617 was confirmed by FISH. Dual-luciferase reporter assay and rescue experiments were conducted to investigate the role of the circSIPA1L1/miR-617/Smad3 regulatory axis in osteogenesis of DPSCs.Results: Sanger sequencing and back-to-back primer experiments confirmed the closed loop structure of circSIPA1L1. CircSIPA1L1 could promote the committed differentiation of DPSCs. MiR-617 was predicted to be the target binding circSIPA1L1 through MiRDB, miRTarBase, and TargetScan database analyses, which was further confirmed by dual-luciferase reporter assay. FISH results showed that circSIPA1L1 and miR-617 colocalize in the cytoplasm of DPSCs. MiR-617 exerted an inhibitory effect on osteogenesis of DPSCs. Knockdown of circSIPA1L1 or upregulation of miR-617 down-regulated phosphorylated Smad3. In addition, rescue experiments showed that knockdown of miR-617 reversed the inhibitory effect of circSIPA1L1 on osteogenesis of DPSCs.Conclusion: CircRNASIPA1L1 promotes osteogenesis of DPSCs by adsorbing miR-617 and further targeting Smad3.