Simultaneous Quantification of Hederacoside C and α-hederin in Hedera Nepalensis K.Koch Using HPLC-UV
Authors: Thi Thuy Linh Do , Thanh Duong Hoang , Tuan Hiep Nguyen , Thanh Huyen Pham , Minh Khoi Nguyen , Doan Long Dinh 
Published: September 2020

This study develops a high performance liquid chromatography with ultraviolet detection (HPLC-UV) for simultaneous quantification of hederacoside C and α-hederin in Hedera nepalensis K. Koch. The method proposed in this study was validated in terms of the analytical parameters such as high repeatability, high accuracy and good sensitivity. The method was used to determine the content of hederacoside C and α-hederin in Hedera nepalensis K. Koch, which had been collected in Ha Giang, Lao Cai and Lai Chau. The study results show that the content of hederacoside C and the content of α-hederin ranged from 0.40 to 4.01% and 0.21 – 0.54% based on absolute dry mass, respectively.
Keywords
Hedera nepalensis K. Koch, hederacoside C, α-hederin, HPLC-UV.
References
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