bluuebunny/crossref_metadata_2025_split huggingface dataset:
The recurrence rate of ovarian endometrioma in women aged 40–49 years and impact of hormonal treatment after conservative surgery
Authors: Lee Nara , Min Seunggi , Won Seyeon , Cho Yeon Jean , Kim Miseon , Kim Mi Kyoung , Jung Yong Wook , Yun Bo Seong , Seong Seok Ju , Kim Mi-La 
Published: October 2020

AbstractThe aim of this study was to evaluate the rate of and risk factors for recurrence ovarian endometrioma after conservative surgery in patients aged 40–49 years. This retrospective, single-center study included 408 women between January 2008 and November 2018. All patients underwent ovarian cyst enucleation, were pathologically diagnosed with ovarian endometrioma and were followed up for ≥ 6 months. Recurrence was defined as a cystic mass with diameter ≥ 2 cm detected by sonography. Recurrence rate after conservative surgery and risk factor of recurrence were analyzed. The median follow-up duration after surgery was 32.0 ± 25.9 months (range 6–125 months). Ovarian endometrioma recurred in 34 (8.3%) of included women and median time to recurrence was 22.4 ± 18.2 months. The cumulative recurrences rate at 12, 24, 36, and 60 months were 3.7%, 6.7%, 11.1%, and 16.7%, respectively. Recurrence was correlated with multilocular cysts (p = 0.038), previous surgical history of ovarian endometrioma (p = 0.006) and salpingectomy (p = 0.043), but not use or duration of post-operative medication. In multivariate analysis, large cyst size (> 5.5 cm) was only risk factor for recurrence in this age group. Post-operative medication did not reduce disease recurrence rate, and thus may be administered for endometriosis-associated pain rather than to prevent recurrence in patients aged 40–49 years.
Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative substrate binding
Authors: Hall Jenny L. , Sohail Azmat , Cabrita Eurico J. , Macdonald Colin , Stockner Thomas , Sitte Harald H. , Angulo Jesus , MacMillan Fraser 
Published: October 2020

AbstractSaturation-transfer difference (STD) NMR spectroscopy is a fast and versatile method which can be applied for drug-screening purposes, allowing the determination of essential ligand binding affinities (KD). Although widely employed to study soluble proteins, its use remains negligible for membrane proteins. Here the use of STD NMR for KD determination is demonstrated for two competing substrates with very different binding affinities (low nanomolar to millimolar) for an integral membrane transport protein in both detergent-solubilised micelles and reconstituted proteoliposomes. GltPh, a homotrimeric aspartate transporter from Pyrococcus horikoshii, is an archaeal homolog of mammalian membrane transport proteins—known as excitatory amino acid transporters (EAATs). They are found within the central nervous system and are responsible for fast uptake of the neurotransmitter glutamate, essential for neuronal function. Differences in both KD’s and cooperativity are observed between detergent micelles and proteoliposomes, the physiological implications of which are discussed.
Combination therapy of cold atmospheric plasma (CAP) with temozolomide in the treatment of U87MG glioblastoma cells
Authors: Gjika Eda , Pal-Ghosh Sonali , Kirschner Megan E. , Lin Li , Sherman Jonathan H. , Stepp Mary Ann , Keidar Michael 
Published: October 2020

AbstractCold atmospheric plasma (CAP) technology, a relatively novel technique mainly investigated as a stand-alone cancer treatment method in vivo and in vitro, is being proposed for application in conjunction with chemotherapy. In this study, we explore whether CAP, an ionized gas produced in laboratory settings and that operates at near room temperature, can enhance Temozolomide (TMZ) cytotoxicity on a glioblastoma cell line (U87MG). Temozolomide is the first line of treatment for glioblastoma, one of the most aggressive brain tumors that remains incurable despite advancements with treatment modalities. The cellular response to a single CAP treatment followed by three treatments with TMZ was monitored with a cell viability assay. According to the cell viability results, CAP treatment successfully augmented the effect of a cytotoxic TMZ dose (50 μM) and further restored the effect of a non-cytotoxic TMZ dose (10 μM). Application of CAP in conjunction TMZ increased DNA damage measured by the phosphorylation of H2AX and induced G2/M cell cycle arrest. These findings were supported by additional data indicating reduced cell migration and increased αvβ3 and αvβ5 cell surface integrin expression as a result of combined CAP–TMZ treatment. The data presented in this study serve as evidence that CAP technology can be a suitable candidate for combination therapy with existing chemotherapeutic drugs. CAP can also be investigated in future studies for sensitizing glioblastoma cells to TMZ and other drugs available in the market.
FFA2-, but not FFA3-agonists inhibit GSIS of human pseudoislets: a comparative study with mouse islets and rat INS-1E cells
Authors: Lorza-Gil Estela , Kaiser Gabriele , Rexen Ulven Elisabeth , König Gabriele M. , Gerst Felicia , Oquendo Morgana Barroso , Birkenfeld Andreas L. , Häring Hans-Ulrich , Kostenis Evi , Ulven Trond , Ullrich Susanne 
Published: October 2020

AbstractThe expression of short chain fatty acid receptors FFA2 and FFA3 in pancreatic islets raised interest in using them as drug targets for treating hyperglycemia in humans. This study aims to examine the efficacy of synthetic FFA2- and FFA3-ligands to modulate glucose-stimulated insulin secretion (GSIS) in human pseudoislets which display intact glucose responsiveness. The FFA2-agonists 4-CMTB and TUG-1375 inhibited GSIS, an effect reversed by the FFA2-antagonist CATPB. GSIS itself was not augmented by CATPB. The FFA3-agonists FHQC and 1-MCPC did not affect GSIS in human pseudoislets. For further drug evaluation we used mouse islets. The CATPB-sensitive inhibitory effect of 100 µM 4-CMTB on GSIS was recapitulated. The inhibition was partially sensitive to the Gi/o-protein inhibitor pertussis toxin. A previously described FFA2-dependent increase of GSIS was observed with lower concentrations of 4-CMTB (10 and 30 µM). The stimulatory effect of 4-CMTB on secretion was prevented by the Gq-protein inhibitor FR900359. As in human pseudoislets, in mouse islets relative mRNA levels were FFAR2 > FFAR3 and FFA3-agonists did not affect GSIS. The FFA3-agonists, however, inhibited GSIS in a pertussis toxin-sensitive manner in INS-1E cells and this correlated with relative mRNA levels of Ffar3 > > Ffar2. Thus, in humans, when FFA2-activation impedes GSIS, FFA2-antagonism may reduce glycemia.
Cdc6 disruption leads to centrosome abnormalities and chromosome instability in pancreatic cancer cells
Authors: Youn Yuna , Lee Jong-chan , Kim Jaihwan , Kim Jae Hyeong , Hwang Jin-Hyeok 
Published: October 2020

AbstractCell division cycle 6 (Cdc6) plays key roles in regulating DNA replication, and activation and maintenance of cell cycle check points. In addition, Cdc6 exerts oncogenic properties via genomic instability associated with incomplete DNA replication. This study aimed to examine the effects of Cdc6 on pancreatic cancer (PC) cells. Our results showed that Cdc6 expression was higher in clinical PC specimens (based on analysis of the GEPIA database) and cell lines, and the high Cdc6 expression was associated with poorer survival in The Cancer Genome Atlas-PC cohort. In addition, Cdc6-depleted PC cells significantly inhibited cell proliferation and colony formation, delayed G2/M cell cycle progression, and increased expression of p-histone H3 and cyclin A2 levels. These observations could be explained by Cdc6 depletion leading to multipolar and split spindles via centrosome amplification and microtubule disorganization which eventually increases chromosome missegregation. Furthermore, Cdc6-depleted PC cells showed significantly increased apoptosis, which was consistent with increased caspase-9 and caspase-3 activation. Collectively, our results demonstrated that Cdc6-depleted PC cells are arrested in mitosis and eventually undergo cell death by induced multipolar spindles, centrosome aberrations, microtubule disorganization, and chromosome instability. In conclusion, Cdc6 may be a potential biomarker and therapeutic target for PC.
Miniaturization re-establishes symmetry in the wing folding patterns of featherwing beetles
Authors: Petrov Pyotr N. , Farisenkov Sergey E. , Polilov Alexey A. 
Published: October 2020

AbstractMost microinsects have feather-like bristled wings, a state known as ptiloptery, but featherwing beetles (family Ptiliidae) are unique among winged microinsects in their ability to fold such wings. An asymmetrical wing folding pattern, found also in the phylogenetically related rove beetles (Staphylinidae), was ancestral for Ptiliidae. Using scanning electron, confocal laser scanning, and optical microscopy, high-speed video recording, and 3D reconstruction, we analyze in detail the symmetrical wing folding pattern and the mechanism of the folding and unfolding of the wings in Acrotrichis sericans (Coleoptera: Ptiliidae) and show how some of the smaller featherwing beetles have reverted to strict symmetry in their wing folding. The wings are folded in three phases by bending along four lines (with the help of wing folding patches on the abdominal tergites) and locked under the closed elytra; they unfold passively in two phases, apparently with the help of the elasticity provided by resilin unevenly distributed in the wing and of convexities forming in the cross-sections of the unfolding wing, making it stiffer. The minimum duration of folding is 3.5 s; unfolding is much more rapid (minimum duration lowest recorded in beetles, 0.038 s). The folding ratio of A. sericans is 3.31 (without setae), which is greater than in any beetle in which it has been measured. The symmetrical wing folding pattern found in A. sericans and in all of the smallest ptiliids, in which ptiloptery is especially pronounced, is the only known example of symmetry re-established during miniaturization. This direction of evolution is remarkable because miniaturization is known to result in various asymmetries, while in this case miniaturization was accompanied by reversal to symmetry, probably associated with the evolution of ptiloptery. Our results on the pattern and mechanisms of wing folding and unfolding can be used in robotics for developing miniature biomimetic robots: the mechanisms of wing folding and unfolding in Ptiliidae present a challenge to engineers who currently work at designing ever smaller flying robots and may eventually produce miniature robots with foldable wings.
The effect of slope aspect on vegetation attributes in a mountainous dry valley, Southwest China
Authors: Yang Jie , El-Kassaby Yousry A. , Guan Wenbin 
Published: October 2020

AbstractSlope aspect plays a critical role in influencing vegetation pattern in semiarid area. The dry valleys of the Hengduan Mountains Region, southwestern China, are striking geographical landscape, suffering from severe ecological degradation. Here, we comprehensively investigated how slope aspect affects vegetation attributes in one of these valleys- the dry valley in the upper reaches of Min River. Three sites were selected along the valley and we quantitively examined the vegetation difference between slope aspects at the whole valley scale and each site level. We found significant vegetation differences between slope aspects in species composition, vegetative structure, and biodiversity pattern, which were in accordance with the observed significant difference in soil nutrient. Generally, north-facing slopes are associated with higher biomass, coverage and height, and species diversity than south-facing slopes. We also found between-aspect differences varied among the study sites, resulting in increased biomass, height, and β diversity differences, decreased density and coverage differences, and opposite trend observed in α diversity at relatively wet site. In conclusion, slope aspect had significant effect on vegetation attributes, which was significantly influenced by local climate (aridity) in terms of both strength and direction depending on the specific attributes investigated.
Lectin affinity chromatography and quantitative proteomic analysis reveal that galectin-3 is associated with metastasis in nasopharyngeal carcinoma
Authors: Aimjongjun Sathid , Reamtong Onrapak , Janvilisri Tavan 
Published: October 2020

AbstractNasopharyngeal carcinoma (NPC) is a serious cancer in East and Southeast Asia. Patients are often diagnosed at advanced stages, rendering treatment failure due to high potential of metastasis. This study identified lectin-binding glycoproteins with a potential role in NPC metastasis. Cell lysate and culture medium in highly metastatic 5-8F, and lowly-metastatic 6-10B NPC cell lines were fractionated by ConA- and WGA-affinity chromatography, and subjected to GeLC-MS/MS. A total of 232 and 197 proteins were identified in ConA-enriched fraction of 5-8F and 6-10B cell lysates respectively. In WGA-enriched fraction, 65 and 164 proteins were found in 5-8F and 6-10B cell lysates respectively. Proteins identified in culture medium for both cell lines were 223 and 85 for ConA-enriched fraction, and 94 and 124 for WGA-enriched fraction from 5-8F and 6-10B respectively. Differentially expressed proteins were functionally categorized into cell–cell adhesion, extracellular matrix, glycolysis, protein homeostasis and/or glycosylation enzymes, and lipid metabolism. Interestingly, Galectin-3 (Gal-3) was highly expressed in 5-8F cells but was lowly expressed in 6-10B cells. The Gal-3 knockdown in 5-8F cells, Gal-3 overexpression in 6-10B cells and treatment with Gal-3 inhibitor revealed that Gal-3 was responsible for metastatic phenotypes including adhesion, migration and invasion. So Galectin-3 may serve as a potential target for NPC therapeutic interventions.
Rainwater isotopes in central Vietnam controlled by two oceanic moisture sources and rainout effects
Authors: Wolf Annabel , Roberts William H. G. , Ersek Vasile , Johnson Kathleen R. , Griffiths Michael L. 
Published: October 2020

AbstractThe interpretation of palaeoclimate archives based on oxygen isotopes depends critically on a detailed understanding of processes controlling the isotopic composition of precipitation. In the summer monsoonal realm, like Southeast Asia, seasonally and interannually depleted oxygen isotope ratios in precipitation have been linked to the summer monsoon strength. However, in some regions, such as central Vietnam, the majority of precipitation falls outside the summer monsoon period. We investigate processes controlling stable isotopes in precipitation from central Vietnam by combining moisture uptake calculations with monthly stable isotope data observed over five years. We find that the isotopic seasonal cycle in this region is driven by a shift in moisture source from the Indian Ocean to the South China Sea. This shift is reflected in oxygen isotope ratios with low values (− 8 to − 10‰) during summer and high values during spring/winter (0 to − 3‰), while 70% of the annual rainfall occurs during autumn. Interannual changes in precipitation isotopes in central Vietnam are governed by the timing of the seasonal onset and withdrawal of the Intertropical Convergence Zone, which controls the amount of vapour contributed from each source.
3D DESI-MS lipid imaging in a xenograft model of glioblastoma: a proof of principle
Authors: Henderson Fiona , Jones Emrys , Denbigh Joanna , Christie Lidan , Chapman Richard , Hoyes Emmy , Claude Emmanuelle , Williams Kaye J. , Roncaroli Federico , McMahon Adam 
Published: October 2020

AbstractDesorption electrospray ionisation mass spectrometry (DESI-MS) can image hundreds of molecules in a 2D tissue section, making it an ideal tool for mapping tumour heterogeneity. Tumour lipid metabolism has gained increasing attention over the past decade; and here, lipid heterogeneity has been visualised in a glioblastoma xenograft tumour using 3D DESI-MS imaging. The use of an automatic slide loader automates 3D imaging for high sample-throughput. Glioblastomas are highly aggressive primary brain tumours, which display heterogeneous characteristics and are resistant to chemotherapy and radiotherapy. It is therefore important to understand biochemical contributions to their heterogeneity, which may be contributing to treatment resistance. Adjacent sections to those used for DESI-MS imaging were used for H&E staining and immunofluorescence to identify different histological regions, and areas of hypoxia. Comparing DESI-MS imaging with biological staining allowed association of different lipid species with hypoxic and viable tissue within the tumour, and hence mapping of molecularly different tumour regions in 3D space. This work highlights that lipids are playing an important role in the heterogeneity of this xenograft tumour model, and DESI-MS imaging can be used for lipid 3D imaging in an automated fashion to reveal heterogeneity, which is not apparent in H&E stains alone.
The immune receptor CD300e negatively regulates T cell activation by impairing the STAT1-dependent antigen presentation
Authors: Coletta Sara , Salvi Valentina , Della Bella Chiara , Bertocco Ambra , Lonardi Silvia , Trevellin Elisabetta , Fassan Matteo , D’Elios Mario M. , Vermi William , Vettor Roberto , Cagnin Stefano , Sozzani Silvano , Codolo Gaia , de Bernard Marina 
Published: October 2020

AbstractCD300e is a surface receptor, expressed by myeloid cells, involved in the tuning of immune responses. CD300e engagement was reported to provide the cells with survival signals, to trigger the expression of activation markers and the release of pro-inflammatory cytokines. Hence, CD300e is considered an immune activating receptor. In this study, we demonstrate that the ligation of CD300e in monocytes hampers the expression of the human leukocyte antigen (HLA) class II, affecting its synthesis. This effect, which is associated with the transcription impairment of the signal transducer and activator of transcription 1 (STAT1), overcomes the capacity of interferon gamma (IFN-γ) to promote the expression of the antigen-presenting molecules. Importantly, the decreased expression of HLA-II on the surface of CD300e-activated monocytes negatively impacts their capacity to activate T cells in an antigen-specific manner. Notably, unlike in vitro- differentiated macrophages which do not express CD300e, the immune receptor is expressed by tissue macrophages. Taken together, our findings argue against the possibility that this molecule should be considered an activating immune receptor sensu stricto. Moreover, our results support the notion that CD300e might be a new player in the regulation of the expansion of T cell-mediated responses.
Relationships between a common Caribbean corallivorous snail and protected area status, coral cover, and predator abundance
Authors: Shaver Elizabeth C. , Renzi Julianna J. , Bucher Maite G. , Silliman Brian R. 
Published: October 2020

AbstractAs coral populations decline across the Caribbean, it is becoming increasingly important to understand the forces that inhibit coral survivorship and recovery. Predation by corallivores, such as the short coral snail Coralliophila abbreviata, are one such threat to coral health and recovery worldwide, but current understanding of the factors controlling corallivore populations, and therefore predation pressure on corals, remains limited. To examine the extent to which bottom-up forces (i.e., coral prey), top-down forces (i.e., predators), and marine protection relate to C. abbreviata distributions, we surveyed C. abbreviata abundance, percent coral cover, and the abundance of potential snail predators across six protected and six unprotected reefs in the Florida Keys. We found that C. abbreviata abundance was lower in protected areas where predator assemblages were also more diverse, and that across all sites snail abundance generally increased with coral cover. C. abbreviata abundance had strong, negative relationships with two gastropod predators—the Caribbean spiny lobster (Panulirus argus) and the grunt black margate (Anisotremus surinamensis), which may be exerting top-down pressure on C. abbreviata populations. Further, we found the size of C. abbreviata was also related to reef protection status, with larger C. abbreviata on average in protected areas, suggesting that gape-limited predators such as P. argus and A. surinamensis may alter size distributions by targeting small snails. Combined, these results provide preliminary evidence that marine protection in the Florida Keys may preserve critical trophic interactions that indirectly promote coral success via control of local populations of the common corallivorous snail C. abbreviata.
Short wavelength automated perimetry and standard automated perimetry in central serous chorioretinopathy
Authors: Zhou Han Peng , Asaoka Ryo , Inoue Tatsuya , Asano Shotaro , Murata Hiroshi , Hara Takumi , Makino So , Kadonosono Kazuaki , Obata Ryo 
Published: October 2020

AbstractShort wavelength automated perimetry (SWAP) is known for detecting the early reduction of retinal sensitivity (RS) in glaucoma. It’s application in retinal diseases have also been discussed previously. We investigated the difference in RS measured between standard white-on-white automated perimetry (WW) and blue-on-yellow SWAP in central serous chorioretinopathy (CSC). The overall RS (W-RS, S-RS) as well as the RS inside and outside of the serous retinal detachment (SRD) region were investigated in 26 eyes of 26 CSC patients using WW and SWAP. The central retinal thickness, central choroidal thickness, SRD area (SRDa), and SRD height at the fovea were measured using optic coherence tomography. RS inside the SRD region was lower than that of outside for both perimetries (both p < 0.001). The difference between RS inside and outside of the SRD region was greater in SWAP compared to WW (p < 0.001). Univariate analysis revealed significant correlations between SRDa and both W-RS and S-RS (both p < 0.001); moreover, multivariate analysis indicated that only S-RS was selected as the optimal model for SRDa. Our study demonstrated that SWAP was detected the decrease in RS more accurately than WW in CSC. These results may suggest the usefulness of SWAP for detecting change of retinal function in CSC.
IL-4R alpha deficiency influences hippocampal-BDNF signaling pathway to impair reference memory
Authors: Brombacher T. M. , Berkiks I. , Pillay S. , Scibiorek M. , Moses B. O. , Brombacher F. 
Published: October 2020

AbstractLike pro-inflammatory cytokines, the role of anti-inflammatory cytokines in both learning and memory has been investigated, revealing beneficial effects for both interleukin-4 and interleukin-13 via the common interleukin-4 receptor alpha chain complex. In this study, using the Morris water maze spatial task for cognition, we compared interleukin-4 receptor alpha- deficient mice and their ligands interleukin-4/ interleukin-13 double deficient mice, on a Balb/c background. We demonstrate that while interleukin-4/ interleukin-13 double deficient mice are significantly impaired in both learning and reference memory, interleukin-4 receptor alpha-deficiency impairs only reference memory, compared to the wild-type control mice. In order to better understand how interleukin-4 receptor alpha- deficient mice are able to learn but not remember, we investigated the BDNF/TrkB- and the ARC-signaling pathways. We show that interleukin-4 receptor alpha-deficiency disrupts activation of BDNF/TrkB- and ARC-signaling pathways during reference memory, while the pathway for spatial learning is spared.