bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of a food neophobia test to characterize personality traits of dairy calves
Authors: Costa Joao H. C. , Neave Heather W. , Weary Daniel M. , von Keyserlingk Marina A. G. 
Published: April 2020

AbstractFood neophobia, i.e. the avoidance of novel foods, is common in ruminants and may provide a biologically relevant and practical way to test individual responses to novelty or challenge. We aimed to determine if behavioural responses in a food neophobia test (exposure to a novel total mixed ration) reflected boldness and exploratory personality traits derived from 3 traditional tests (open field, novel human and novel object) in dairy calves. We performed two Principal Component Analyses, one using behaviours from 3 traditional tests (3 factors: ‘Bold’, ‘Exploratory’ and ‘Active’), and one using behaviours from the food neophobia test (3 factors: ‘Eating’, ‘Inspecting’, and ‘Avoidance’). A regression analysis determined if individual factor scores from the food neophobia test predicted factor scores from the traditional tests. Contrary to our expectations, ‘Avoidance’ (latencies to approach and eat the novel food) did not predict boldness trait, and the factors ‘Inspecting’ (time spent inspecting food and empty buckets) and ‘Eating’ (time spent eating food and total intake) did not predict exploration trait, but they did predict active trait. These results suggest that the food neophobia test in our study resulted in context-specific behaviours, or that behavioural responses to a novel food present different underlying personality traits. The application of food neophobia to assess specific or generalized personality traits of dairy calves deserves further work.
Biological and molecular characterization of Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae), an emerging pest of stone fruits in Europe
Authors: Russo Elia , Nugnes Francesco , Vicinanza Francesco , Garonna Antonio P. , Bernardo Umberto 
Published: April 2020

AbstractThe red-necked longhorn beetle (RLB) Aromia bungii (Fald.) is an emerging pest of stone fruit trees, native to East Asia, accidentally introduced in Europe (Germany and Italy) and Japan. Threatening seriously the stone fruit crops in Europe, RLB was added to both the EPPO A1 and priority pest lists of quarantine species. Molecular analyses highlighted that all specimens recovered in southern Italy share the same haplotype, different from the German one, supporting that the invasive process in Europe started from at least two independent introductions. To fill the existing gap of biological knowledge about A. bungii, several laboratory tests were carried out on specimens collected in the outbreak area of Naples (Italy). Results suggest a high biotic potential of the RLB Italian population. Females showed a short pre-oviposition period while the period of oviposition lasted about three weeks, with a rate of 24.2 eggs/day. Each female laid an average of 587.5 eggs and spawned the largest amount of eggs during the first week after emergence. Fed males live up to 62 days at 20 °C while fed females about 63 days at 25 °C. These results are crucial to draw up a multi-facet IPM approach against A. bungii in the outbreak areas.
First proton minibeam radiation therapy treatment plan evaluation
Authors: Lansonneur P. , Mammar H. , Nauraye C. , Patriarca A. , Hierso E. , Dendale R. , Prezado Y. , De Marzi L. 
Published: April 2020

AbstractProton minibeam radiation therapy (pMBRT) is a novel dose delivery method based on spatial dose fractionation. pMBRT has been shown to be promising in terms of reduced side effects and superior tumour control in high-grade glioma-bearing rats compared to standard irradiation. These findings, together with the recent optimized implementation of pMBRT in a clinical pencil beam scanning system, have triggered reflection on the possible application to patient treatments. In this context, the present study was designed to conduct a first theoretical investigation of the clinical potential of this technique. For this purpose, a dedicated dose engine was developed and used to evaluate two clinically relevant patient treatment plans (high-grade glioma and meningioma). Treatment plans were compared with standard proton therapy plans assessed by means of a commercial treatment planning system (ECLIPSE-Varian Medical systems) and Monte Carlo simulations. A multislit brass collimator consisting of 0.4 mm wide slits separated by a centre-to-centre distance of 4 or 6 mm was placed between the nozzle and the patient to shape the planar minibeams. For each plan, spread-out Bragg peaks and homogeneous dose distributions (±7% dose variations) can be obtained in target volumes. The Peak-to-Valley Dose Ratios (PVDR) were evaluated between 9.2 and 12.8 at a depth of 20 mm for meningioma and glioma, respectively. Dose volume histograms (DVHs) for target volumes and organs at risk were quantitatively compared, resulting in a slightly better target homogeneity with standard PT than with pMBRT plans, but similar DVHs for deep-seated organs-at-risk and lower average dose for shallow organs. The proposed delivery method evaluated in this work opens the way to an effective treatment for radioresistant tumours and will support the design of future clinical research.
Enhanced dynamic functional connectivity (whole-brain chronnectome) in chess experts
Authors: Premi Enrico , Gazzina Stefano , Diano Matteo , Girelli Andrea , Calhoun Vince D. , Iraji Armin , Gong Qiyong , Li Kaiming , Cauda Franco , Gasparotti Roberto , Padovani Alessandro , Borroni Barbara , Magoni Mauro 
Published: April 2020

AbstractMultidisciplinary approaches have demonstrated that the brain is potentially modulated by the long-term acquisition and practice of specific skills. Chess playing can be considered a paradigm for shaping brain function, with complex interactions among brain networks possibly enhancing cognitive processing. Dynamic network analysis based on resting-state magnetic resonance imaging (rs-fMRI) can be useful to explore the effect of chess playing on whole-brain fluidity/dynamism (the chronnectome). Dynamic connectivity parameters of 18 professional chess players and 20 beginner chess players were evaluated applying spatial independent component analysis (sICA), sliding-time window correlation, and meta-state approaches to rs-fMRI data. Four indexes of meta-state dynamic fluidity were studied:i)the number of distinct meta-states a subject pass through,ii)the number of switches from one meta-state to another,iii)the span of the realized meta-states (the largest distance between two meta-states that subjects occupied), andiv)the total distance travelled in the state space. Professional chess players exhibited an increased dynamic fluidity, expressed as a higher number of occupied meta-states (meta-state numbers, 75.8 ± 7.9 vs 68.8 ± 12.0, p = 0.043 FDR-corrected) and changes from one meta-state to another (meta-state changes, 77.1 ± 7.3 vs 71.2 ± 11.0, p = 0.043 FDR-corrected) than beginner chess players. Furthermore, professional chess players exhibited an increased dynamic range, with increased traveling between successive meta-states (meta-state total distance, 131.7 ± 17.8 vs 108.7 ± 19.7, p = 0.0004 FDR-corrected). Chess playing may induce changes in brain activity through the modulation of the chronnectome. Future studies are warranted to evaluate if these potential effects lead to enhanced cognitive processing and if “gaming” might be used as a treatment in clinical practice.
Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing
Authors: Fraiture Marie-Alice , Bogaerts Bert , Winand Raf , Deckers Marie , Papazova Nina , Vanneste Kevin , De Keersmaecker Sigrid C. J. , Roosens Nancy H. C. 
Published: April 2020

AbstractRecently, the unexpected presence of a viable unauthorized genetically modified bacterium in a commercialized food enzyme (protease) product originating from a microbial fermentation process has been notified at the European level (RASFF 2019.3332). This finding was made possible thanks to the use of the next-generation sequencing technology, as reported in this study. Whole-genome sequencing was used to characterize the genetic modification comprising a sequence from the pUB110 shuttle vector (GenBank: M19465.1), harbouring antimicrobial resistance genes conferring a resistance to kanamycine, neomycin and bleomycin, flanked on each side by a sequence coding for a protease (GenBank: WP_032874795.1). In addition, based on these data, two real-time PCR methods, that can be used by enforcement laboratories, specific to this unauthorized genetically modified bacterium were developed and validated. The present study emphasizes the key role that whole-genome sequencing can take for detection of unknown and unauthorized genetically modified microorganisms in commercialized microbial fermentation products intended for the food and feed chain. Moreover, current issues encountered by the Competent Authorities and enforcement laboratories with such unexpected contaminations and the importance of performing official controls were highlighted.
N-Myc-induced metabolic rewiring creates novel therapeutic vulnerabilities in neuroblastoma
Authors: Tjaden Britta , Baum Katharina , Marquardt Viktoria , Simon Mareike , Trajkovic-Arsic Marija , Kouril Theresa , Siebers Bettina , Lisec Jan , Siveke Jens T. , Schulte Johannes H. , Benary Uwe , Remke Marc , Wolf Jana , Schramm Alexander 
Published: April 2020

AbstractN-Myc is a transcription factor that is aberrantly expressed in many tumor types and is often correlated with poor patient prognosis. Recently, several lines of evidence pointed to the fact that oncogenic activation of Myc family proteins is concomitant with reprogramming of tumor cells to cope with an enhanced need for metabolites during cell growth. These adaptions are driven by the ability of Myc proteins to act as transcriptional amplifiers in a tissue-of-origin specific manner. Here, we describe the effects of N-Myc overexpression on metabolic reprogramming in neuroblastoma cells. Ectopic expression of N-Myc induced a glycolytic switch that was concomitant with enhanced sensitivity towards 2-deoxyglucose, an inhibitor of glycolysis. Moreover, global metabolic profiling revealed extensive alterations in the cellular metabolome resulting from overexpression of N-Myc. Limited supply with either of the two main carbon sources, glucose or glutamine, resulted in distinct shifts in steady-state metabolite levels and significant changes in glutathione metabolism. Interestingly, interference with glutamine-glutamate conversion preferentially blocked proliferation of N-Myc overexpressing cells, when glutamine levels were reduced. Thus, our study uncovered N-Myc induction and nutrient levels as important metabolic master switches in neuroblastoma cells and identified critical nodes that restrict tumor cell proliferation.
Chitinase 3-like 1 is neurotoxic in primary cultured neurons
Authors: Matute-Blanch Clara , Calvo-Barreiro Laura , Carballo-Carbajal Iria , Gonzalo Ricardo , Sanchez Alex , Vila Miquel , Montalban Xavier , Comabella Manuel 
Published: April 2020

AbstractChitinase 3-like 1 (CHI3L1) is known to play a role as prognostic biomarker in the early stages of multiple sclerosis (MS), and patients with high cerebrospinal fluid CHI3L1 levels have an increased risk for the development of neurological disability. Here, we investigated its potential neurotoxic effect by adding recombinant CHI3L1 in vitro to primary cultures of mouse cortical neurons and evaluating both neuronal functionality and survival by immunofluorescence. CHI3L1 induced a significant neurite length retraction after 24 and 48 hours of exposure and significantly reduced neuronal survival at 48 hours. The cytotoxic effect of CHI3L1 was neuron-specific and was not observed in mouse immune or other central nervous system cells. These results point to a selective neurotoxic effect of CHI3L1 in vitro and suggest a potential role of CHI3L1 as therapeutic target in MS patients.
Role of plants in the transmission of Asaia sp., which potentially inhibit the Plasmodium sporogenic cycle in Anopheles mosquitoes
Authors: Bassene Hubert , Niang El Hadji Amadou , Fenollar Florence , Doucoure Souleymane , Faye Ousmane , Raoult Didier , Sokhna Cheikh , Mediannikov Oleg 
Published: April 2020

AbstractBiological control against malaria and its transmission is currently a considerable challenge. Plant-associated bacteria of the genus Asaia are frequently found in nectarivorous arthropods, they thought to have a natural indirect action on the development of plasmodium in mosquitoes. However, virtually nothing is known about its natural cycle. Here, we show the role of nectar-producing plants in the hosting and dissemination of Asaia. We isolated Asaia strains from wild mosquitoes and flowers in Senegal and demonstrated the transmission of the bacteria from infected mosquitoes to sterile flowers and then to 26.6% of noninfected mosquitoes through nectar feeding. Thus, nectar-producing plants may naturally acquire Asaia and then colonize Anopheles mosquitoes through food-borne contamination. Finally, Asaia may play an indirect role in the reduction in the vectorial capacity of Anopheles mosquitoes in a natural environment (due to Plasmodium-antagonistic capacities of Asaia) and be used in the development of tools for Asaia-based paratransgenetic malaria control.
Molecular mechanisms of synergistic action of Ramucirumab and Paclitaxel in Gastric Cancers cell lines
Authors: Refolo Maria Grazia , Lotesoriere Claudio , Lolli Ivan Roberto , Messa Caterina , D’Alessandro Rosalba 
Published: April 2020

AbstractRamucirumab is approved both as monotherapy and in combination with Paclitaxel for advanced gastric cancer in patients with disease progression after chemotherapy. In tumor cells, the VEGFA-VEGFR2 binding activates autocrine survival and migration signaling in angiogenesis independent manner. The present in vitro study investigated the effects of single and combined treatments with Ramucirumab and Paclitaxel on cell growth and migration highlighting the mechanisms underlying the interaction between the two drugs in gastric cancer cells. Cell growth and motility were investigated in human gastric cancer cell lines characterized by different tumorigenicity. The inhibitory effect on cell growth exerted by both drugs was potentiated by their combination and was synergistic. Ramucirumab was able to enhance the inhibitory effect exerted by Paclitaxel on cell cycle progression. A synergistic action was also observed in the expression of proteins crucial for cell motility, microtubule organization and epithelial-mesenchymal transition. Furthermore, synergistic inhibition of VEGFR2 expression was obtained by the drug combination. These findings highlighted the importance of the combined treatment to strongly inhibit all the main molecules of both PI3K/Akt/mTOR and MAPK pathways thus preventing possible reactivations due to cross-talk phenomena. The combined treatment with Ramucirumab seems to be a promising option to overcome the Paclitaxel resistance.
Amino acid 159 of the envelope protein affects viral replication and T-cell infiltration by West Nile virus in intracranial infection
Authors: Kobayashi Shintaro , Kaneko Chisato , Kawakami Ryoko , Hasebe Rie , Sawa Hirofumi , Yoshii Kentaro , Kariwa Hiroaki 
Published: April 2020

AbstractWest Nile virus (WNV) is an important cause of viral encephalitis in birds and animals, including humans. Amino acid 159 of the envelope (E) protein is reportedly implicated in the different levels of neurovirulence in mice infected with WNV NY99 or Eg101. We investigated the role of amino acid 159 of the E protein in the pathogenesis of WNV infection. We produced recombinant WNV with the structural proteins of the NY99 or Eg101 strain (NY-WT or EgCME-WT) and mutant viruses with substitutions of amino acid 159 of the E protein (NY-E-V159I or EgCME-E-I159V). The NY-WT and NY-E-V159I or EgCME-WT and EgCME-E-I159V titers in culture supernatant were similar. The mortality rate and viral titer in the brains of mice inoculated intraperitoneally with NY-WT or NY-E-V159I were also similar. In contrast, the mortality rate and viral titer in the brains of mice inoculated intracranially with EgCME-E-I159V were significantly higher than those of mice inoculated with EgCME-WT. The numbers of CD3-positive and CD8-positive T cells were greater in brains inoculated with EgCME-E-I159V than in those inoculated with EgCME-WT. Therefore, amino acid 159 of the E protein modulates the pathogenicity of WNV by affecting viral replication and T-cell infiltration in the brain.
Caloric restriction reverses left ventricular hypertrophy through the regulation of cardiac iron homeostasis in impaired leptin signaling mice
Authors: An Hyeong Seok , Lee Jong Youl , Choi Eun Bee , Jeong Eun Ae , Shin Hyun Joo , Kim Kyung Eun , Park Kyung-Ah , Jin Zhen , Lee Jung Eun , Koh Jin Sin , Kwak Woori , Kim Won-Ho , Roh Gu Seob 
Published: April 2020

AbstractLeptin-deficient and leptin-resistant mice manifest obesity, insulin resistance, and left ventricular hypertrophy (LVH); however, LVH’s mechanisms are not fully understood. Cardiac iron dysregulation has been recently implicated in cardiomyopathy. Here we investigated the protective effects of caloric restriction on cardiac remodeling in impaired leptin signaling obese mice. RNA-seq analysis was performed to assess the differential gene expressions in the heart of wild-type and ob/ob mice. In particular, to investigate the roles of caloric restriction on iron homeostasis-related gene expressions, 10-week-old ob/ob and db/db mice were assigned to ad libitum or calorie-restricted diets for 12 weeks. Male ob/ob mice exhibited LVH, cardiac inflammation, and oxidative stress. Using RNA-seq analysis, we identified that an iron uptake-associated gene, transferrin receptor, was upregulated in obese ob/ob mice with LVH. Caloric restriction attenuated myocyte hypertrophy, cardiac inflammation, fibrosis, and oxidative stress in ob/ob and db/db mice. Furthermore, we found that caloric restriction reversed iron homeostasis-related lipocalin 2, divalent metal transporter 1, transferrin receptor, ferritin, ferroportin, and hepcidin expressions in the heart of ob/ob and db/db mice. These findings demonstrate that the cardioprotective effects of caloric restriction result from the cellular regulation of iron homeostasis, thereby decreasing oxidative stress, inflammation, and cardiac remodeling. We suggest that decreasing iron-mediated oxidative stress and inflammation offers new therapeutic approaches for obesity-induced cardiomyopathy.
Enhancement of chemical oscillations by self-generated convective flows
Authors: Shklyaev Oleg E. , Yashin Victor V. , Stupp Samuel I. , Balazs Anna C. 
Published: April 2020

AbstractChemical feedback loops in fluids can produce not only chemical oscillations, but also density variations that generate solutal buoyancy forces, which in turn initiate fluid flow. Using analytical and computational models, we herein examine how the reaction-induced flows alter the chemical oscillations in a fluid-filled chamber whose top and bottom walls are coated with different enzymes. Due to this chemo-fluidic coupling, the systems form oscillating flow patterns, which combine the characteristic size of the buoyancy-driven convection rolls with the frequency of the chemical oscillations. With changes in the distance between the enzyme-coated walls, the convective flows not only enhance or suppress the chemical oscillations, but also substantially increase the amplitude and frequency of the oscillations and extend the regime of the oscillatory behavior. These design principles can facilitate the development of artificial biochemical networks that act as chemical clocks.
TESS first look at evolved compact pulsators
Authors: Bognár Zs. , Kawaler S. D. , Bell K. J. , Schrandt C. , Baran A. S. , Bradley P. A. , Hermes J. J. , Charpinet S. , Handler G. , Mullally S. E. , Murphy S. J. , Raddi R. , Sódor Á. , Tremblay P.-E. , Uzundag M. , Zong W. 
Published: April 2020

Context. We present our findings on 18 previously known ZZ Ceti stars observed by the TESS space telescope in 120 s cadence mode during the survey observation of the southern ecliptic hemisphere.
Aims. We focus on the frequency analysis of the space-based observations, comparing the results with findings of previous ground-based measurements. The frequencies detected by the TESS observations can serve as inputs for future asteroseismic analyses.
Methods. We performed standard pre-whitening of the data sets to derive the possible pulsation frequencies of the different targets. In some cases, we fit Lorentzians to the frequency groups that emerged as the result of short-term amplitude or phase variations that occurred during the TESS observations.
Results. We detected more than 40 pulsation frequencies in seven ZZ Ceti stars observed in the 120 s cadence by TESS, with precision better than 0.1 μHz. We found that HE 0532−5605 may be a new outbursting ZZ Ceti. Ten targets do not show any significant pulsation frequencies in their Fourier transforms, due to a combination of their intrinsic faintness and/or crowding on the large TESS pixels. We also detected possible amplitude or phase variations during the TESS observations in some cases. Such behaviour in these targets was not previously identified from ground-based observations.
Dynamical cloud formation traced by atomic and molecular gas
Authors: Beuther H. , Wang Y. , Soler J. , Linz H. , Henshaw J. , Vazquez-Semadeni E. , Gomez G. , Ragan S. , Henning Th. , Glover S. C. O. , Lee M.-Y. , Güsten R. 
Published: April 2020

Context.Atomic and molecular cloud formation is a dynamical process. However, kinematic signatures of these processes are still observationally poorly constrained.Aims.We identify and characterize the cloud formation signatures in atomic and molecular gas.Methods.Targeting the cloud-scale environment of the prototypical infrared dark cloud G28.3, we employed spectral line imaging observations of the two atomic lines HI and [CI] as well as molecular lines observations in13CO in the 1–0 and 3–2 transitions. The analysis comprises investigations of the kinematic properties of the different tracers, estimates of the mass flow rates, velocity structure functions, a histogram of oriented gradients (HOG) study, and comparisons to simulations.Results.The central infrared dark cloud (IRDC) is embedded in a more diffuse envelope of cold neutral medium traced by HI self-absorption and molecular gas. The spectral line data as well as the HOG and structure function analysis indicate a possible kinematic decoupling of the HI from the other gas compounds. Spectral analysis and position–velocity diagrams reveal two velocity components that converge at the position of the IRDC. Estimated mass flow rates appear rather constant from the cloud edge toward the center. The velocity structure function analysis is consistent with gas flows being dominated by the formation of hierarchical structures.Conclusions.The observations and analysis are consistent with a picture where the IRDC G28.3 is formed at the center of two converging gas flows. While the approximately constant mass flow rates are consistent with a self-similar, gravitationally driven collapse of the cloud, external compression (e.g., via spiral arm shocks or supernova explosions) cannot be excluded yet. Future investigations should aim at differentiating the origin of such converging gas flows.