bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loading influence on the corrosion assessment during stress-induced martensite reorientation in nickel-titanium SMA
Authors: Mohajeri Mahdi , Case Raymundo , Haghgouyan Behrouz , Lagoudas Dimitris C , Castaneda Homero 
Published: January 2020

Abstract
The effect of stress-induced martensite reorientation on the corrosion behavior of a nickel-titanium (NiTi) shape memory alloy (SMA) was characterized by mechanical-electrochemical techniques. This martensite reorientation influenced the passive state of NiTi SMA. These passive to active state transitions could be sensed by the global interfacial electrochemical measurements, such as electrochemical impedance spectroscopy (EIS), open circuit potential (OCP) and linear polarization resistance (LPR) methods. The increase in the loading when exposed to a corrosive environment resulted in the breakdown of the passive layer. A later repassivation stage was reached due to the formation rate becoming more dominant than the breakdown rate during loading conditions. The integration of the mechanical loading by tensile testing, strain distribution by digital image correlation (DIC) and electrochemical methods by OCP and EIS, characterized and detected the influence of the mechanical effect in the corrosion assessment and interfacial mechanisms.
Dynamic phase fluctuations in potential-driven Bose–Einstein condensate
Authors: Ma Decheng , Koval Vladimir , Jia Chenglong 
Published: January 2020

Abstract
We report on the dynamics of a Bose–Einstein condensate in one and two dimensions driven by the time-dependent harmonic trapping potential. Without the inter-particle interaction, the condensate exhibits the coherent behavior with the time-oscillating density distribution. When the inter-particle interaction is taken into consideration, the phase fluctuations, the multi-peak structure of the density distribution, and the coherence revival phenomenon, apart from the well-studied density oscillation behavior, can be observed in the condensate. Furthermore, it is demonstrated that due to the dimensional restriction these effects are more stable in the two and three-dimensional system, if compared to the one-dimensional case.
Effects of changes in land use on the diurnal temperature range: a long-term data analysis
Authors: Ueno Kazuki , Ohta Shunji 
Published: January 2020

Abstract
Air temperature is sensitive to changes in local land use. In this study, we investigated the effect of dam lake water on the air temperature based on open climate data. Lake Sayama (and its surroundings), located in Tokorozawa City, 30 km from Tokyo, Japan, was undertaken as the case study. This artificial lake was empty for three years for seismic reinforcement. To remove the effects of time-series changes in the air temperature, nearby observation stations where the air temperature difference did not change from that of Tokorozawa during the analysis period were selected. Analysis of data revealed that the minimum air temperature increased, and the maximum air temperature slightly decreased during the period when the dam reservoir was full throughout the year. Moreover, the reduction in the diurnal range of air temperatures was 1.78 °C in December and 0.46 °C in August. The effect of the lake on the air temperature reflected the size of the lake and the climatic zone. These results highlight the need for maintaining a network of meteorological observation stations, whose data can prove useful for identifying local climate changes due to land use.
Compliant bipolar electrostatic gripper using 3D-printed-layered elastic probes
Authors: Hemthavy Pasomphone , Kudo Kenta , Kawano Kento , Takahashi Kunio , Saito Shigeki 
Published: January 2020

Abstract
A 3D-printed-layered structure for forming the compliant bipolar electrostatic gripper is proposed. A prototype gripper module, which consists of an array of elastically deformable bipolar micro-probes, is fabricated using a conventional 3D printer. Experiments are conducted to examine the attractive force and the pick-and-place performance of the prototype. Experimental results show that the prototype module can generate the maximum attractive pressure of 87.1 Pa, which can pick and hold a piece of xerographic paper of 31.2 mg successfully. The effects of residual charges and the probe-tips flattening process on the attractive force are also discussed.
PamgeneAnalyzeR: open and reproducible pipeline for kinase profiling
Authors: Bekkar Amel , Nasrallah Anita , Guex Nicolas , Fajas Lluis , Xenarios Ioannis , Lopez-Mejia Isabel C 
Published: January 2020

Abstract

Protein phosphorylation––catalyzed by protein kinases–is the most common post-translational modification. It increases the functional diversity of the proteome and influences various aspects of normal physiology and can be altered in disease states. High throughput profiling of kinases is becoming an essential experimental approach to investigate their activity and this can be achieved using technologies such as PamChip® arrays provided by PamGene for kinase activity measurement. Here, we present ‘pamgeneAnalyzeR’, an R package developed as an alternative to the manual steps necessary to extract the data from PamChip® peptide microarrays images in a reproducible and robust manner. The extracted data can be directly used for downstream analysis.

Availability and implementation
PamgeneAnalyzeR is implemented in R and can be obtained from https://github.com/amelbek/pamgeneAnalyzeR.
A comprehensive in vivo and mathematic modeling-based kinetic characterization for aspirin-induced chemoprevention in colorectal cancer
Authors: Shimura Tadanobu , Toden Shusuke , Komarova Natalia L , Boland Crichard , Wodarz Dominik , Goel Ajay 
Published: January 2020

Abstract
Accumulating evidence suggests that aspirin has anti-tumorigenic properties in colorectal cancer (CRC). Herein, we undertook a comprehensive and systematic series of in vivo animal experiments followed by 3D-mathematical modeling to determine the kinetics of aspirin’s anti-cancer effects on CRC growth. In this study, CRC xenografts were generated using four CRC cell lines with and without PIK3CA mutations and microsatellite instability, and the animals were administered with various aspirin doses (0, 15, 50, and 100 mg/kg) for 2 weeks. Cell proliferation, apoptosis and protein expression were evaluated, followed by 3D-mathematical modeling analysis to estimate cellular division and death rates and their impact on aspirin-mediated changes on tumor growth. We observed that aspirin resulted in a dose-dependent decrease in the cell division rate, and a concomitant increase in the cell death rates in xenografts from all cell lines. Aspirin significantly inhibited cell proliferation as measured by Ki67 staining (P &lt; 0.05–0.01). The negative effect of aspirin on the rate of tumor cell proliferation was more significant in xenograft tumors derived from PIK3CA mutant versus wild-type cells. A computational model of 3D-tumor growth suggests that the growth inhibitory effect of aspirin on the tumor growth kinetics is due to a reduction of tumor colony formation, and that this effect is sufficiently strong to be an important contributor to the reduction of CRC incidence in aspirin-treated patients. In conclusion, we provide a detailed kinetics of aspirin-mediated inhibition of tumor cell proliferation, which support the epidemiological data for the observed protective effect of aspirin in CRC patients.
Synaptic Zinc Enhances Inhibition Mediated by Somatostatin, but not Parvalbumin, Cells in Mouse Auditory Cortex
Authors: Kouvaros Stylianos , Kumar Manoj , Tzounopoulos Thanos 
Published: January 2020

AbstractCortical inhibition is essential for brain activity and behavior. Yet, the mechanisms that modulate cortical inhibition and their impact on sensory processing remain less understood. Synaptically released zinc, a neuromodulator released by cortical glutamatergic synaptic vesicles, has emerged as a powerful modulator of sensory processing and behavior. Despite the puzzling finding that the vesicular zinc transporter (ZnT3) mRNA is expressed in cortical inhibitory interneurons, the actions of synaptic zinc in cortical inhibitory neurotransmission remain unknown. Using in vitro electrophysiology and optogenetics in mouse brain slices containing the layer 2/3 (L2/3) of auditory cortex, we discovered that synaptic zinc increases the quantal size of inhibitory GABAergic neurotransmission mediated by somatostatin (SOM)- but not parvalbumin (PV)-expressing neurons. Using two-photon imaging in awake mice, we showed that synaptic zinc is required for the effects of SOM- but not PV-mediated inhibition on frequency tuning of principal neurons. Thus, cell-specific zinc modulation of cortical inhibition regulates frequency tuning.
Prevalence and Incidence of Human Papillomavirus Infection in Men Having Sex With Men Enrolled in a Pre-exposure Prophylaxis Study: A Sub-study of the Agence Nationale de Recherches sur le SIDA et les Hépatites Virales “Intervention Préventive de l’Exposition aux Risques avec et pour les hommes Gays” Trial
Authors: Cotte Laurent , Veyer David , Charreau Isabelle , Péré Hélène , Cua Eric , Carette Diane , Chas Julie , Capitant Catherine , Chidiac Christian , Fléjou Jean-François , Fouéré Sébastien , Heard Isabelle , Meyer Laurence , Puech Julien , Tremblay Cécile , Delaugerre Constance , Molina Jean-Michel 
Published: January 2020

Abstract

Background
Human papillomavirus (HPV) infection is more frequent in men having sex with men (MSM) who are living with human immunodeficiency virus (HIV) than in MSM without HIV. There are currently no data regarding HPV infections in preexposure prophylaxis (PrEP)-using MSM.

Methods
MSM living without HIV who were enrolled in the Agence Nationale de Recherches sur le SIDA et les Hépatites Virales "Intervention Préventive de l'Exposition aux Risques avec et pour les hommes Gays" PrEP study were prospectively enrolled. Anal, penile, and oral samples were collected at baseline and every 6 months for HPV detection and genotyping. Anal swabs for cytology were obtained at baseline and at 24 months.

Results
We enrolled 162 participants. The prevalences of any HPV genotypes at baseline were 92%, 32%, and 12% at the anal, penile, and oral sites, respectively. High-risk (HR) HPV genotypes were observed in 84%, 25%, and 10% of anal, penile, and oral baseline samples, respectively. Nonavalent HPV vaccine genotypes were observed in 77%, 22%, and 6% of anal, penile, and oral baseline samples, respectively. Multiple infections were observed in 76%, 17%, and 3% of cases at the anal, penile, and oral sites, respectively. The most frequent HR genotypes were HPV 53, 51, and 16 in anal samples; HPV 33, 39, and 73 in penile samples; and HPV 66 in oral samples. The incidence of any HPV genotype at the anal site was 86.2/1000 person-months and the incidence of HR-HPV genotypes was 72.3/1000 person-months. The baseline cytology was normal in 32% of cases and was classified as atypical squamous cells of undetermined significance, low-grade squamous intra-epithelial lesion, high-grade squamous intra-epithelial lesion (HSIL), and atypical squamous cells that cannot exclude HSIL in 23%, 40%, 5%, and 1% of cases, respectively.

Conclusions
PrEP users have a similar risk of HPV infection as MSM living with HIV and the risk is much higher than that previously reported in MSM living without HIV.
Hepatitis B Virus RNA as Early Predictor for Response to Pegylated Interferon Alpha in HBeAg-Negative Chronic Hepatitis B
Authors: Farag Mina S , van Campenhout Margo J H , Pfefferkorn Maria , Fischer Janett , Deichsel Danilo , Boonstra André , van Vuuren Anneke J , Ferenci Peter , Feld Jordan J , Berg Thomas , Hansen Bettina E , van Bömmel Florian , Janssen Harry L A 
Published: January 2020

Abstract

Background
Hepatitis B virus RNA (HBV-RNA) is a novel serum biomarker that correlates with transcription of intrahepatic covalently closed circular (cccDNA), which is an important target for pegylated interferon (PEG-IFN) and novel therapies for functional cure. We studied HBV-RNA kinetics following PEG-IFN treatment and its potential role as a predictor to response in HBeAg-negative chronic hepatitis B (CHB) patients.

Methods
HBV-RNA levels were measured in 133 HBeAg-negative CHB patients treated in an international randomized controlled trial (PARC study). Patients received PEG-IFN α-2a for 48 weeks. HBV-RNA was measured from baseline through week 144. Response was defined as HBV-DNA &lt;2000 IU/mL and ALT normalization at week 72. Kinetics of HBV-RNA were compared with HBV-DNA, HBsAg, and HBcrAg.

Results
Mean HBV-RNA at baseline was 4.4 (standard deviation [SD] 1.2) log10 c/mL. At week 12, HBV-RNA declined by −1.6 (1.1) log10 c/mL. HBV-RNA showed a greater decline in responders compared to nonresponders early at week 12 (−2.0 [1.2] vs −1.5 [1.1] log10 c/mL, P = .04). HBV-RNA level above 1700 c/mL (3.2 log10 c/mL) had a negative predictive value of 91% at week 12 and 93% at week 24 (P = .01) for response. Overall, HBV-RNA showed a stronger correlation with HBV-DNA and HBcrAg (.82 and .80, P &lt; .001) and a weak correlation with HBsAg (.25). At week 12, HBV-RNA was significantly lower among patients with lower HBsAg (&lt;100 IU/mL) or HBsAg loss at week 144.

Conclusions
During PEG-IFN treatment for HBeAg-negative CHB, HBV-RNA showed a fast and significant decline that correlates with treatment response and HBsAg loss at long-term follow-up.

Clinical Trials Registration
NCT00114361
Kt/V: achievement, predictors and relationship to mortality in hemodialysis patients in the Gulf Cooperation Council countries: results from DOPPS (2012–18)
Authors: AlSahow Ali , Muenz Daniel , Al-Ghonaim Mohammed A , Al Salmi Issa , Hassan Mohamed , Al Aradi Ali H , Hamad Abdullah , Al-Ghamdi Saeed M G , Shaheen Faissal A M , Alyousef Anas , Bieber Brian , Robinson Bruce M , Pisoni Ronald L 
Published: January 2020

AbstractBackgroundDialysis adequacy, as measured by single pool Kt/V, is an important parameter for assessing hemodialysis (HD) patients’ health. Guidelines have recommended Kt/V of 1.2 as the minimum dose for thrice-weekly HD. We describe Kt/V achievement, its predictors and its relationship with mortality in the Gulf Cooperation Council (GCC) (Bahrain, Kuwait, Oman, Qatar, Saudi Arabia and the United Arab Emirates).MethodsWe analyzed data (2012–18) from the prospective cohort Dialysis Outcomes and Practice Patterns Study for 1544 GCC patients ≥18 years old and on dialysis &gt;180 days.ResultsThirty-four percent of GCC HD patients had low Kt/V (&lt;1.2) versus 5%–17% in Canada, Europe, Japan and the USA. Across the GCC countries, low Kt/V prevalence ranged from 10% to 54%. In multivariable logistic regression, low Kt/V was more common (P &lt; 0.05) with larger body weight and height, being male, shorter treatment time (TT), lower blood flow rate (BFR), greater comorbidity burden and using HD versus hemodiafiltration. In adjusted Cox models, low Kt/V was strongly related to higher mortality in women [hazard ratio (HR) = 1.91, 95% confidence interval (CI) 1.09–3.34] but not in men (HR = 1.16, 95% CI 0.70–1.92). Low BFR (&lt;350 mL/min) and TT (&lt;4 h) were common; 41% of low Kt/V cases were attributable to low BFR or TT (52% for women and 36% for men).ConclusionRelatively large proportions of GCC HD patients have low Kt/V. Increasing BFR to ≥350 mL/min and TT to ≥4 h thrice weekly will reduce low Kt/V prevalence and may improve survival in GCC HD patients—particularly among women.
The Organizational Field of Blood Collection: A Multilevel Analysis of Organizational Determinants of Blood Donation in Europe
Authors: Gorleer Sam , Bracke Piet , Hustinx Lesley 
Published: January 2020

AbstractMaintaining an adequate blood supply for transfusion poses a pressing challenge to society. We argue that this challenge has not been adequately addressed in previous research. Building upon Healy’s seminal work on ‘blood-collection regimes’ and the subsequent shift towards a field-level approach that broadens the analytical focus beyond the dyadic relationship between donors and organizations, we embed the act of blood donation within the organizational field in which blood establishments operate. We assume that varying modes of governance shape the organizational practices of donor recruitment and blood collection. Our analysis is based on Eurobarometer data from 2014 (number of countries = 28; number of individuals = 19,363). The results identify considerable variance in donation rates according to field characteristics in terms of hierarchical centralization and competitiveness. Decentralized systems without competition perform worst in terms of the recruitment of (first-time) blood donors. Competitive systems in which several different bodies share responsibility for the provision of blood to patients yield the highest donation rates.
Termination of persistent atrial fibrillation by ablating sites that control large atrial areas
Authors: Bhatia Neal K , Rogers Albert J , Krummen David E , Hossainy Samir , Sauer William , Miller John M , Alhusseini Mahmood I , Peszek Adam , Armenia Erin , Baykaner Tina , Brachmann Johannes , Turakhia Mintu P , Clopton Paul , Wang Paul J , Rappel Wouter-Jan , Narayan Sanjiv M 
Published: January 2020

Abstract

Aims
Persistent atrial fibrillation (AF) has been explained by multiple mechanisms which, while they conflict, all agree that more disorganized AF is more difficult to treat than organized AF. We hypothesized that persistent AF consists of interacting organized areas which may enlarge, shrink or coalesce, and that patients whose AF areas enlarge by ablation are more likely to respond to therapy.

Methods and results
We mapped vectorial propagation in persistent AF using wavefront fields (WFF), constructed from raw unipolar electrograms at 64-pole basket catheters, during ablation until termination (Group 1, N = 20 patients) or cardioversion (Group 2, N = 20 patients). Wavefront field mapping of patients (age 61.1 ± 13.2 years, left atrium 47.1 ± 6.9 mm) at baseline showed 4.6 ± 1.0 organized areas, each separated by disorganization. Ablation of sites that led to termination controlled larger organized area than competing sites (44.1 ± 11.1% vs. 22.4 ± 7.0%, P &lt; 0.001). In Group 1, ablation progressively enlarged unablated areas (rising from 32.2 ± 15.7% to 44.1 ± 11.1% of mapped atrium, P &lt; 0.0001). In Group 2, organized areas did not enlarge but contracted during ablation (23.6 ± 6.3% to 15.2 ± 5.6%, P &lt; 0.0001).

Conclusion
Mapping wavefront vectors in persistent AF revealed competing organized areas. Ablation that progressively enlarged remaining areas was acutely successful, and sites where ablation terminated AF were surrounded by large organized areas. Patients in whom large organized areas did not emerge during ablation did not exhibit AF termination. Further studies should define how fibrillatory activity is organized within such areas and whether this approach can guide ablation.
Barriers and facilitators to GP–patient communication about emotional concerns in UK primary care: a systematic review
Authors: Parker Daisy , Byng Richard , Dickens Chris , Kinsey Debbie , McCabe Rose 
Published: January 2020

AbstractBackgroundIn the UK, general practitioners (GPs) are the most commonly used providers of care for emotional concerns.ObjectiveTo update and synthesize literature on barriers and facilitators to GP–patient communication about emotional concerns in UK primary care.DesignSystematic review and qualitative synthesis.MethodWe conducted a systematic search on MEDLINE (OvidSP), PsycInfo and EMBASE, supplemented by citation chasing. Eligible papers focused on how GPs and adult patients in the UK communicated about emotional concerns. Results were synthesized using thematic analysis.ResultsAcross 30 studies involving 342 GPs and 720 patients, four themes relating to barriers were: (i) emotional concerns are difficult to disclose; (ii) tension between understanding emotional concerns as a medical condition or arising from social stressors; (iii) unspoken assumptions about agency resulting in too little or too much involvement in decisions and (iv) providing limited care driven by little time. Three facilitative themes were: (v) a human connection improves identification of emotional concerns and is therapeutic; (vi) exploring, explaining and negotiating a shared understanding or guiding patients towards new understandings and (vii) upfront information provision and involvement manages expectations about recovery and improves engagement in treatment.ConclusionThe findings suggest that treatment guidelines should acknowledge: the therapeutic value of a positive GP–patient relationship; that diagnosis is a two-way negotiated process rather than an activity strictly in the doctor’s domain of expertise; and the value of exploring and shaping new understandings about patients’ emotional concerns and their management.
SPECC1L regulates palate development downstream of IRF6
Authors: Hall Everett G , Wenger Luke W , Wilson Nathan R , Undurty-Akella Sraavya S , Standley Jennifer , Augustine-Akpan Eno-Abasi , Kousa Youssef A , Acevedo Diana S , Goering Jeremy P , Pitstick Lenore , Natsume Nagato , Paroya Shahnawaz M , Busch Tamara D , Ito Masaaki , Mori Akihiro , Imura Hideto , Schultz-Rogers Laura E , Klee Eric W , Babovic-Vuksanovic Dusica , Kroc Sarah A , Adeyemo Wasiu L , Eshete Mekonen A , Bjork Bryan C , Suzuki Satoshi , Murray Jeffrey C , Schutte Brian C , Butali Azeez , Saadi Irfan 
Published: January 2020

Abstract
SPECC1L mutations have been identified in patients with rare atypical orofacial clefts and with syndromic cleft lip and/or palate (CL/P). These mutations cluster in the second coiled-coil and calponin homology domains of SPECC1L and severely affect the ability of SPECC1L to associate with microtubules. We previously showed that gene-trap knockout of Specc1l in mouse results in early embryonic lethality. We now present a truncation mutant mouse allele, Specc1lΔC510, that results in perinatal lethality. Specc1lΔC510/ΔC510 homozygotes showed abnormal palate rugae but did not show cleft palate. However, when crossed with a gene-trap allele, Specc1lcGT/ΔC510 compound heterozygotes showed a palate elevation delay with incompletely penetrant cleft palate. Specc1lcGT/ΔC510 embryos exhibit transient oral epithelial adhesions at E13.5, which may delay shelf elevation. Consistent with oral adhesions, we show periderm layer abnormalities, including ectopic apical expression of adherens junction markers, similar to Irf6 hypomorphic mutants and Arhgap29 heterozygotes. Indeed, SPECC1L expression is drastically reduced in Irf6 mutant palatal shelves. Finally, we wanted to determine if SPECC1L deficiency also contributed to non-syndromic (ns) CL/P. We sequenced 62 Caucasian, 89 Filipino, 90 Ethiopian, 90 Nigerian and 95 Japanese patients with nsCL/P and identified three rare coding variants (p.Ala86Thr, p.Met91Iso and p.Arg546Gln) in six individuals. These variants reside outside of SPECC1L coiled-coil domains and result in milder functional defects than variants associated with syndromic clefting. Together, our data indicate that palate elevation is sensitive to deficiency of SPECC1L dosage and function and that SPECC1L cytoskeletal protein functions downstream of IRF6 in palatogenesis.