bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole‐exome sequencing reveals novel vacuolar ATPase genes’ variants and variants in genes involved in lysosomal biology and autophagosomal formation in oral granular cell tumors
Authors: França Josiane Alves , Gayden Tenzin , Bareke Eric , Santos Jean Nunes , de Sousa Sílvia Ferreira , Bastos‐Rodrigues Luciana , Majewski Jacek , Jabado Nada , Gomez Ricardo Santiago , Gomes Carolina Cavalieri 
Published: December 2020

AbstractBackgroundGranular cell tumors (GCTs) are rare neuroectodermal soft tissue neoplasms that mainly affect the skin of the upper limbs and trunks and the oral cavity. GCTs are derived from Schwann cells and, ultrastructurally, their intracytoplasmic granules are considered autophagosomes or autophagolysosomes and are consistent with myelin accumulation.MethodsIn this study, a convenience set of 22 formalin‐fixed, paraffin‐embedded samples of oral GCTs, all but one sample located at the tongue, was screened for mutations by whole‐exome (WES) or targeted sequencing.ResultsWES revealed two novel variants in genes of the vacuolar ATPase (V‐ATPase) complex: ATP6AP1 frameshift c.746_749del, leading to p.P249Hfs*4, and ATP6V1A non‐synonymous c.G868A, leading to p.D290N. Each of these mutations occurred in one case. With regard to the samples that were wild type for these V‐ATPase variants, at least two samples presented variants in genes that are part of endosomal/lysosomal/autophagosomal networks including ABCA8, ABCC6, AGAP3, ATG9A, CTSB, DNAJC13, GALC, NPC1, SLC15A3, SLC31A2, and TMEM104.ConclusionAlthough the mechanisms involved in oral GCT initiation and progression remain unclear, our results suggest that oral GCTs have V‐ATPase variants similarly to GCTs from other tissues/organs, and additionally show variants in lysosomes/endosomes/autophagosomal genes.
Use of diaries as a research strategy on sensory perception and consumer behavior of Canastra cheese
Authors: Rodrigues Jessica Ferreira , Siman Ingrid Brandemburg , de Oliveira Lorena Eduarda Aparecida , de Fátima Barcelos Alessandra , Oliveira Rhaí André Arriel , Silva Ramon , da Cruz Adriano Gomes 
Published: December 2020

AbstractThe diary method has been used in some areas of knowledge, being uncommon in the area of sensory analysis of food. In addition, given the expanding market and the added value of Canastra artisanal Minas cheese, it is important to study consumer behavior, as well as understand the consumption habits of the product. Thus, the objective of this study was to use the diary method to collect sensory data, in addition to investigating the behavior and perceptions of consumers of artisanal Minas cheese. Potentials and weaknesses of the application of the method were discussed. The results of this study will contribute to sensory science, focusing on the assessment of real and continued food consumption. Moreover, they provide a better understanding of consumer behavior and consumption habits of artisanal Minas cheese from Canastra, directing planning production, marketing and commercialization strategies.Practical ApplicationThe findings of this study are useful for Brazilian artisanal cheese producers as it enables understanding the consumer behavior and consumption habits of artisanal cheese to plan production, marketing, and commercialization strategies. In addition, it shows practical utility for consumer‐based methodologies, focusing on the assessment of real and continued food consumption.
The clinical value of holmium laser enucleation of the prostate in octogenarians
Authors: Tamalunas Alexander , Westhofen Thilo , Schott Melanie , Keller Patrick , Atzler Michael , Stief Christian G. , Magistro Giuseppe 
Published: December 2020

AbstractObjectivesWith holmium laser enucleation of the prostate (HoLEP) a size‐independent method for surgical treatment of lower urinary tract symptoms (LUTS) secondary to benign prostatic obstruction (BPO) has been introduced. HoLEP offers durable long‐term results with reduced perioperative morbidity. As the risk of disease progression increases with age, the main goals, when offering surgery to an elderly population, are reducing perioperative morbidity and preserving quality of life (QoL). We therefore analyzed the impact of age on outcomes and perioperative morbidity in patients undergoing HoLEP for LUTS at our tertiary referral center.MethodsWe retrospectively collected data of 487 patients who underwent HoLEP for LUTS secondary to BPO between 2018 and 2019. Patients were divided into group 1 (<70 years), group 2 (70‐79 years), and group 3 (≥80 years). Perioperative parameters, safety, and short‐term functional outcomes were assessed and analyzed.ResultsPerioperative Clavien‐Dindo grade ≥II complications were seen in 4.1% of patients (20/487). There was no difference in perioperative complications between all age groups (P = .176). Functional outcome was assessed 30 days post surgery. There was significant improvement in median International Prostate Symptom Score of 14, 10, and 8 points for groups 1, 2, and 3 (P < .001), respectively, with constant improvement of median QoL of 3 points for all groups. Median maximum flow rate (Qmax) showed significant improvement of 14.5, 10.5, and 13 mL/s for groups 1 to 3 (P = .467), respectively.ConclusionHoLEP offers acceptable perioperative complication rates even in the oldest patient cohort (≥80 years). Therefore, HoLEP is a safe and efficient option even in oldest patients.
Brain potentials reveal differential processing of masculine and feminine grammatical gender in native Spanish speakers
Authors: Beatty‐Martínez Anne L. , Bruni Michelle R. , Bajo María Teresa , Dussias Paola E. 
Published: December 2020

AbstractStudies of Spanish grammatical gender have shown that native speakers exploit gender cues in determiners to facilitate speech processing and are sensitive to gender mismatches. However, past research has not considered attested distributional asymmetries between masculine and feminine gender, collapsing performance on trials with one or the other gender into a single analysis. We use event‐related potentials to investigate whether masculine and feminine grammatical gender elicit qualitatively different brain responses. Forty monolingual Spanish speakers read sentences that were well‐formed or contained determiner‐noun gender violations. Half of the nouns were masculine and the other half were feminine. Consistent with previous research, brain responses varied along a continuum between LAN‐ and P600‐dominant effects for both gender categories. However, results showed that individuals’ ERP response dominance (LAN/P600) systematically differed across the two genders: participants who showed a LAN‐dominant response to masculine‐noun violations were more likely to show a P600 effect in response to feminine‐noun violations. Correlations with individual difference measures further revealed that responses to masculine‐noun violations were modulated by performance on the AX‐CPT, a measure of cognitive control, whereas responses to feminine‐noun violations were modulated by lexical knowledge, as indexed by verbal fluency. Together, the results demonstrate that even when processing features of language that belong to the same “natural class,” native speakers can exhibit patterns of brain activity attuned to distributional patterns of language use. The inherent variability in native speaker processing is, therefore, an important factor when explaining purported deviations from the “native norm” reported in other types of populations.
Upregulated microRNA‐132 in T helper 17 cells activates hepatic stellate cells to promote hepatocellular carcinoma cell migration in vitro
Authors: Feng Rui , Cui Zilin , Liu Zirong , Zhang Yamin 
Published: December 2020

AbstractMicroRNAs play an important role in the modulation of the immune system. T helper 17 (Th17) cells are involved in the modulation of the tumour microenvironment. However, the function of miRNA in Th17 cells in the tumour microenvironment is unclear. In this study, we analysed miR‐132 expression in Th17 cells and assessed the function of miR‐132 on Th17 cell differentiation. In addition, the effect of miR‐132 on Th17 cells in the tumour microenvironment, especially hepatic stellate cells (HSCs), was confirmed. CD4+ IL‐17 ∓ cells were isolated from hepatocellular carcinoma (HCC) tumour tissues. The expression of miR‐132 was higher in CD4+ IL‐17 + cells than in CD4+ IL‐17‐ cells. Human primary CD4+ T cells were used for Th17 cell differentiation. Compared with primary CD4+ T cells, Th17 cells expressed high levels of miR‐132. During Th17 cell differentiation, a miR‐132 mimic and inhibition were applied. After treatment with the miR‐132 mimic, the differentiation of Th17 cells accelerated, showing a a higher percentage of Th17 cells and the expression and secretion of IL‐17 and IL‐22. Smad nuclear interacting protein 1 (SNIP1), as one of the targets of miR‐132, decreased during Th17 cell differentiation–related Th17 differentiation and IL‐17 expression. The conditioned medium of miR‐132–overexpressing Th17 cells could increase the activation of the HSCs, which strongly promoted HCC cell migration and epithelial‐mesenchymal transition (EMT). In summary, miR‐132 positively regulates Th17 cell differentiation and improves the function of Th17 on HSCs for their tumour‐promoting effects.
Stench and sensibilities: On living with waste, animals and microbes in India
Authors: Doron Assa 
Published: December 2020

AbstractStench is often the most immediate mark of something dirty, decaying and diseased. In India, stench and the smell of acrid smoke commonly indicate the proximity of an open dump or landfill. Frequently a slum is located in the vicinity too, housing waste‐pickers who forage in these sprawling dumps for salvageable waste. These spaces are also host to vermin, birds, stray dogs, pigs, cows and, more recently, dangerous bacteria resistant to even top‐end antibiotics, popularly known as ‘superbugs’. In this paper I examine the socio‐ecological context of neighbourhood, community open garbage dumps and larger landfills in an effort to understand these as part of a dynamic ecosystem of ‘more‐than‐human’ relations. Perceptual variations of smell as experienced in and around waste (in its solid, liquid and gas states) are intrinsically linked to symbolic and material practices across species. Additionally, I suggest that one productive way to think about the emergence of disease and pathogenicity is by considering the information stimulated by smell, which is mediated by cultural interpretations, biological capacities and wider political economies.
The PalWRKY77 transcription factor negatively regulates salt tolerance and abscisic acid signaling in Populus
Authors: Jiang Yuanzhong , Tong Shaofei , Chen Ningning , Liu Bao , Bai Qiuxian , Chen Yang , Bi Hao , Zhang Zhiyang , Lou Shangling , Tang Hu , Liu Jianquan , Ma Tao , Liu Huanhuan 
Published: December 2020

SummaryHigh salinity, one of the most widespread abiotic stresses, inhibits photosynthesis, reduces vegetation growth, blocks respiration and disrupts metabolism in plants. In order to survive their long‐term lifecycle, trees, such as Populus species, recruit the abscisic acid (ABA) signaling pathway to adapt to a saline environment. However, the molecular mechanism behind the ABA‐mediated salt stress response in woody plants remains elusive. We have isolated a WRKY transcription factor gene, PalWRKY77, from Populus alba var. pyramidalis (poplar), the expression of which is repressed by salt stress. PalWRKY77 decreases salt tolerance in poplar. Furthermore, PalWRKY77 negatively regulated ABA‐responsive genes and relieved ABA‐mediated growth inhibition, indicating that PalWRKY77 is a repressor of the ABA response. In vivo and in vitro assays revealed that PalWRKY77 targets the ABA‐ and salt‐induced PalNAC002 and PalRD26 genes by binding to the W‐boxes in their promoters. In addition, overexpression of both PalNAC002 and PalRD26 could elevate salt tolerance in transgenic poplars. These findings reveal a novel negative regulation mechanism for the ABA signaling pathway mediated by PalWRKY77 that results in more sensitivity to salt stress in poplar. This deepens our understanding of the complex responses of woody species to salt stress.
Knockout of RSN1, TVP18 or CSC1‐2 causes perturbation of Golgi cisternae in Pichia pastoris
Authors: Aw Rochelle , De Wachter Charlot , Laukens Bram , De Rycke Riet , De Bruyne Michiel , Bell David , Callewaert Nico , Polizzi Karen M. 
Published: December 2020

AbstractThe structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but the role of morphology is less clear in lower eukaryotes. Here we investigated the link between the structural organization of the Golgi and secretory pathway function using Pichia pastoris as a model system. To unstack the Golgi cisternae, we disrupted 18 genes encoding proteins in the secretory pathway without loss of viability. Using biosensors, confocal microscopy and transmission electron microscopy we identified three strains with irreversible perturbations in the stacking of the Golgi cisternae, all of which had disruption in genes that encode proteins with annotated function as or homology to calcium/calcium permeable ion channels. Despite this, no variation in the secretory pathway for ER size, whole cell glycomics or recombinant protein glycans was observed. Our investigations showed the robust nature of the secretory pathway in P. pastoris and suggest that Ca2+ concentration, homeostasis or signalling may play a significant role for Golgi stacking in this organism and should be investigated in other organisms.
Multi-Element Stochastic Galerkin Method Based on Edge Detection for Uncertainty Quantification of Discontinuous Responses
Authors: Kawai Shigetaka , Oyama Akira 
Published: December 2020

Abstract
We propose a new multi-element generalized polynomial chaos (MEgPC) method to minimize the computational costs required for the existing MEgPC to circumvent the Gibbs phenomenon in the presence of discontinuities in a random space. The proposed method uses edge detection to capture the discontinuous behavior of a solution with minimal decomposition. In contrast, the existing MEgPC iterates splitting the random space into two equal parts until achieving a sufficient resolution level. We take advantage of the fact that the stochastic Galerkin (SG) methods facilitate adaptive refinement of the decomposition at every time-step during a computation for the proposed method. The numerical experiments for two-test problems demonstrate the performance of the proposed method. The results show that the proposed method is consistently more accurate than conventional methods for sufficiently high polynomial orders with minimal additional computational costs to capture discontinuities.
On the Effect of the Electrical Load on Vibration Energy Harvesting Under Stochastic Resonance
Authors: Alevras Panagiotis 
Published: December 2020

Abstract
Vibration energy harvesting (VEH) is a promising alternative for powering wireless electronics in many practical applications. Ambient vibration energy in the surrounding space of a target application often involves an inescapable randomness in the exciting vibrations, which may lead to deterioration of the expected power gains due to insufficient tuning and limited optimal designs. Stochastic resonance (SR) is a concept that has recently been considered for exploiting this randomness toward improving power generation from vibrating systems, based on the coexistence of near-harmonic vibrations with broadband noise excitations in a variety of practical mechanical systems. This paper is concerned with the optimal conditions for SR in vibration energy harvesters, exploring the frequently neglected effect of realistic architectures of the electrical circuit on the system dynamics and the achievable power output. A parametric study is conducted using a numerical path integration (PI) method to compute the response probability density functions (PDFs) of vibration energy harvesters, focusing on the effect of standard electrical components; namely, a load resistor, a rectifier, and a capacitor. It is found that the conditions for SR exhibit a nonlinear dependence on the weak harmonic excitation amplitude. Moreover, the modified nonlinear dissipation properties introduced by the rectifier and the capacitor lead to a tradeoff between the power output and the nonconducting dynamics that is essential in order to determine optimal harvesting designs.
INCEFA-PLUS Project: Lessons Learned From the Project Data and Impact on Existing Fatigue Assessment Procedures
Authors: Cuvilliez Sam , McLennan Alec , Mottershead Kevin , Mann Jonathan , Bruchhausen Matthias 
Published: December 2020

Abstract
The INCEFA+ project (INcreasing Safety in nuclear power plants by Covering gaps in Environmental Fatigue Assessment) is a five year project supported by the European Commission HORIZON2020 programme, which will conclude in June 2020. This project aims to generate and analyse Environmental Assisted Fatigue (EAF) experimental data (approximately 230 fatigue data points generated on austenitic stainless steel), and focuses on the effect of several key parameters such as mean strain, hold times and surface finish, and how they interact with environmental effects (air or PWR environment).
This work focuses on the analysis of the data obtained during the INCEFA+ project. More specifically, this paper discusses how the outcome of this analysis can be used to evaluate existing fatigue assessment procedures that incorporate environmental effects in a similar way to NUREG/CR-6909. A key difference between these approaches and the NUREG/CR-6909 is the reduction of conservatisms resulting from the joint implementation of the adjustment sub-factor related to surface finish effect (as quantified in the design air curve derivation) and a Fen penalization factor for fatigue assessment of a location subjected to a PWR primary environment. The analysis presented in this paper indicates that the adjustment (sub-)factor on life associated with the effect of surface finish in air (as described in the derivation of the design air curve in NUREG/CR-6909) leads to substantial conservatisms when it is used to predict fatigue lifetimes in PWR environments for rough specimens. The corresponding margins can be explicitly quantified against the design air curve used for EAF assessment, but may also depend on the environmental correction Fen factor expression that is used to take environmental effects into account.
Effect of Neutron Irradiation on the Mechanical Properties of an A508 Cl.2 Forging Irradiated in a BAMI Capsule
Authors: Uytdenhouwen Inge , Chaouadi Rachid 
Published: December 2020

Abstract
The typical operating temperatures of a nuclear reactor pressure vessel in a PWR is typically between ∼290°C and 300°C. However, many BWRs and some PWRs operate at slightly lower temperatures down to 275°C. Most of the literature and neutron irradiation damage is therefore focused on those irradiation temperatures. It is well-known that the lower the irradiation temperature, the more neutron irradiation damage occurs, because no appreciable annealing occurs at approximately 230°C.
The NOMAD-0 irradiation campaign at the BR2 was a preliminary irradiation specifically designed to determine the appropriate irradiation conditions that result in specific irradiation damage levels of an A508 Cl.2 grade at lower temperatures than the usual PWR operating temperature window. The BAMI capsules with controlled He gas gap were used for this irradiation. To avoid temperature gradients from the outside to the center of the cylindrical blocks, the latter were limited in size. Only tensile and fracture toughness data could therefore be obtained with mini-tensile and mini-CT specimens.
The results show that no influence of the temperature gradient could be found on the tensile and fracture toughness properties. The specimen size and geometry on the fracture toughness results was analyzed and discussed. The effect of irradiation temperature on the tensile properties and the transition temperature as determined by the master curve approach on the mini-CT samples is discussed.
A Feasibility Study on Crack Repair in Austenitic Stainless Steel Dry Storage Canisters Using Isothermal Friction Stir Welding
Authors: Bhattacharyya Madhumanti , Charit Indrajit , Raja Krishnan , Darsell Jens , Jana Saumyadeep 
Published: December 2020

Abstract
In recent time, solid state crack repair techniques in spent nuclear fuel dry storage canisters (SNF-DSC) have garnered significant interest. Plates of austenitic stainless steels are usually arc welded to fabricate the cylindrical canisters which are prone to chloride-induced stress corrosion cracking. Friction stir welding (FSW) is considered to be a potential candidate for damage repair. In this work, electrical discharge machining was used to simulate cracks in 304L stainless steel plates of 12.7 mm thickness. Subsequently, isothermal FSW was carried out at two different temperatures (725 and 825°C) in order to repair the simulated crack. Microstructure-mechanical property correlations in FSWed plates were studied in detail. Significant grain refinement observed in the stir zone (SZ) was attributed to dynamic recrystallization occurring during FSW. A comparison of Vickers microhardness showed higher hardness and homogeneity in the 725°C SZ than its 825°C counterpart. The fraction of Σ3 boundary is found to be low in the SZ as compared to base metal (BM). Yield strength of the joints was found to be approximately 100 MPa higher than that of the BM accompanied by a drop in ductility by a factor of 2. Residual stress profile across 825°C weld was measured using x-ray diffraction.
Insulin use and new diabetes after acceptance for bariatric surgery: comparison of outcomes after completion of surgery or withdrawal from the program
Authors: Lee Jessica H , Jaung Rebekah , Beban Grant , Evennett Nicholas , Cundy Tim 
Published: December 2020

IntroductionIn people accepted onto a bariatric surgery program we compared diabetes-related outcomes in those who completed surgery with those who withdrew before having surgery—examining rates of insulin use in people with type 2 diabetes (T2D), and rates of incident diabetes in people without pre-existing T2D.Research design and methods771 people were accepted onto the program. 463 people (60%) had T2D at referral, of which 48% completed surgery and 52% withdrew. Of 308 people without T2D at referral, 49% completed surgery, and 51% withdrew. Rates of insulin use and incident diabetes were compared by Kaplan-Meier analyses. Among those with pre-existing T2D, we examined rates of remission and relapse after surgery.ResultsPeople without T2D who withdrew from the program had higher mean body mass index and glycated hemoglobin levels than those completing surgery (p<0.005). The rate of incident diabetes at 5 years was 19% in those who withdrew versus 0% in those completing surgery (p<0.001). 30% of people with T2D were taking insulin at referral and all stopped insulin after surgery. During follow-up, the rate of insulin (re)introduction was lower in those who completed surgery (8% vs 26% at 5 years, p<0.001). Of those with T2D who completed surgery, 80% had remission, but 34% had relapsed by 5 years. Diabetes relapse was associated with less weight loss after surgery, a longer duration of T2D and previous insulin use.ConclusionsDespite a high relapse rate, people with T2D who completed surgery had lower insulin use at 5 years than those withdrawing from the program. In people without T2D, bariatric surgery prevented incident diabetes. People without T2D who withdrew from the program were at greater risk of diabetes, suggesting those who could benefit the most in terms of T2D prevention are not completing bariatric surgery.