bluuebunny/crossref_metadata_2025_split huggingface dataset:
PRP-Therapy for Tendinopathies of Rotator Cuff and Long Head of Biceps
Authors: Malanin D. A. , Norkin A. I. , Tregubov A. S. , Demeshchenko M. V. , Cherezov L. L. 
Published: October 2019

Relevance. Owing to its controlling action on the inflammatory process, pain-relieving and reparative effects the RPR-therapy became quite demanded for treatment of certain types of tendinopathies specified by prevalence of degenerative process and poor reparative potential. Purpose of the study — to evaluate the efficiency of PRP-therapy in patients with tendinopathies of rotator cuff (RC) long head of biceps (LHB) tendons. Material and Methods. The paper presents the results of two-center prospective study for application of autologous platelet rich plasma in treatment of 122 patients: 53 men and 69 women aging 46.8±6.8 years who suffered RC tendinopathy (66%), subacromial impingement syndrome and RC tendinopathy (17%) and LHB tendinopathy (17%). Treatment outcomes were evaluated in 1, 3 and 6 months after PRP-therapy using various scales — VAS, UCLA , DASH, — and instrumental examination methods (US, MRI). Results. The authors observed statistically significant improvement in pain and functional scores in all three groups as compared to reported scores prior to PRP-therapy during 6 months follow up. During this period of evaluation, the scores of UCLA and DASH in patients with RC tendinopathy improved at 8.6 and 36.4 points, with subacromial impingement syndrome and RC tendinopathy — at 9.6 and 38.8 points, with LHB tendinopathy — at 11.5 and 44.1 point, respectively. The most notable reduction of pain syndrome by VAS was achieved in treatment of LHB tendinopathy (at 5.4 points) and RC tendinopathy (at 5.2 point). The highest average scores of satisfaction with treatment outcomes were reported in patients with RC tendinopathy (2.3) and LHB tendinopathy (2.2). MRI and ultrasound examinations after PRP-therapy demonstrated improved structure of RC and LHB tendons — decreased or eliminated swelling, areas of hypoechoic signal. Conclusion. PRP-therapy in patients with RC and LHB tendons and with subacromial impingement syndrome with RC tendinopathy significantly reduces severity of pain and improved the shoulder joint function with positive dynamics during 6 months follow up.
Acute Correction of Congenital Brachymetatarsia in Adolescents and Young Adults
Authors: Keshet D. , Salminen S. , Eidelman M. 
Published: October 2019

Introduction. Brachymetatarsia is a relatively rare abnormal shortening of metatarsal bone with female predominance. No consensus exists about the ideal surgical treatment for this condition while the two common strategies are gradual lengthening using external fixation and acute one stage lengthening. We describe the surgical technique of acute lengthening using iliac bone graft and report our results. Materials and Methods. Twelve patients (11 females, one male) with 14 short metatarsals were treated in our hospital over a 12-year period. The mean age was 17.4 years (range 14-28 years). Treatment protocol included metatarsal osteotomy, lengthening of extensor tendon and percutaneous tenotomy of the flexor tendon at the level of affected MP joint. Autologous iliac bone graft was inserted after elongation of the metatarsal by distraction through the osteotomy site and fixed using Kirshner wire. Results. Average metatarsal shortening was 13mm (range, 11-18 mm). The metatarsal length gained was an average of 12mm (range, 11-15 mm). Uneventful bone union was achieved in all cases after six weeks. At the end of treatment, the normal cascade of the metatarsal heads (metatarsal parabola) had been restored in 11 of the 14 metatarsals treated. There were no wound complications or pin tract infections around the wire. All but one patient were satisfied with the cosmetic appearance of the foot. Conclusion. Based on our experience, acute metatarsal lengthening using an iliac autugraft is a reliable and effective treatment method for congenital brachymetatarsia in adolescents and young adults.Level of Evidence: Level IV Case series.
Objective Signs of Foot Deformities in Children with Spastic Form of Cerebral Paralysis: Justification of Individual Approach to Footwear Support
Authors: Smirnova L. M. , Dzhomardly E. I. , Koltsov A. A. 
Published: October 2019

Relevance. Foot deformities are the most common locomotor pathology in children with infantile cerebral paralysis. At the same time many children suffering from this pathology wear standard shoes that do not take into account the individual foot anatomy. Purpose of the study — to justify the expedience of individual approach to orthopaedic support for children with infantile cerebral paralysis. Materials and methods. The authors examined 220 feet in 110 patients aging from 3 to 18 years: 62 patients with spastic form of infantile cerebral paralysis and 48 children who were examined during periodic screening at general education institutions (control group). Clinical examination methods, computerized plantography and podometry by flatbed foot scanning (ventrally, posteriorly, medially) in standing position were used in the present study. Results. Statistically significant variances (p*0.005) were obtained for 8 indicators of foot deformity in three planes in children with infantile cerebral paralysis as compared to the norm, as well as differences between the groups of patients with varying degree of disorders in gross motor functions. The authors established pathological foot deformities in children with infantile cerebral paralysis; statistically significant variances in types and degrees of these disorders for patient groups with different levels of gross motor functions disorders; distinctiveness of foot deformities within each of the groups. Conclusion. Objectively instrumental method was used to identify the main components of foot deformities in patients with infantile cerebral paralysis with preservation and realization of walking capability: loss of height of longitudinal arches, midfoot pronation and hindfoot valgus, hallux valgus. Increased elevation of longitudinal arches (cavus foot), midfoot supination and hindfoot varus are rarer components of deformities occurring more often in patients with severer forms of the pathology. Strong variation in the spread of foot anatomy parameters observed within different groups of motor dysfunctions indicates the expediency of individual approach to footwear recommendations: standard, less or more complex orthopaedic shoes. Implementation of obtained data into the clinical practice requires additional series of biomechanical trials aimed at elaboration of criteria for recommendations and efficiency evaluation of various footwear types that take into account not only specifics of foot anatomy but also its statodynamic function as well as the level of gross motor functions of a particular patient.
A NEW VALIDATED STABILITY-INDICATING DIRECT HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF ROSIGLITAZONE ENANTIOMERS IN THE PRESENCE OF ITS DEGRADATION PRODUCTS
Authors: GOWRAMMA BYRAN , MEYYANATHAN SUBRAMANIYAN NAIYANAR , BABU BASAWAN , KRISHNAVENI NAGAPPAN 
Published: October 2019

Objective: In the present study, an isocratic chiral reverse-phase high-performance liquid chromatography method was developed and the resolution of the drug and complete separation from its degradation products were successfully achieved.
Methods: An isocratic method developed with a Phenomenex Lux 5 μ Cellulose 1 (150 mm×4.6 mm i.d., 5 μ) using UV detector at wavelength of 220 nm, with a mobile phase consisting of methanol:0.1% diethylamine (60:40% v/v) and a flow rate of 1 ml/min. The drug was subjected to alkaline, acidic, neutral, oxidative, and photolytic to apply stress conditions. The stressed samples were analyzed by the proposed method.
Results: The described method was linear over the range of 3–7 μg/ml for R-enantiomer and 9–21 μg/ml of S-enantiomer, respectively. The limit of detection and limit of quantification of R and S enantiomers were found to be 0.56 μg/ml and 0.18 μg/ml, respectively.
Conclusion: The method provides good sensitivity and excellent precision and reproducibility. The developed method can be applied in the quality control of drug products.
IN VITRO CYTOTOXICITY ACTIVITY OF HYDROALCOHOLIC EXTRACT OF POLYHERBAL CHURNAM (COFRI) IN NORMAL LIVER AND KIDNEY CELL LINES
Authors: Y YASMINE , JEBA R CAROLINE 
Published: October 2019

Objective: The objective of the study was to investigate the cytotoxic activity of hydroalcoholic extract of a polyherbal powder on Vero (normal kidney) and Chang (normal liver) cell lines.
Methods: The cytotoxic effect was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide assay. It is commonly used colorimetric assay for assessing cell metabolic activity under specific conditions, indicates the number of viable cells.
Results: According to the experimental results, the hydroalcoholic extract does not cause any toxicity on normal liver and kidney cell lines and LC50 values are ca lculated using LDP line software and found 776.73 μg/ml and 686.58 μg/ml.
Conclusion: The hydroalcoholic extract of polyherbal churnam exhibited cell viability at higher concentrations. The results indicates investigated formulation does not causes any toxicity to the normal liver and kidney cells and found to be safe for human consumption. Further, toxicological and pharmacological evaluation is required to prove its safety and efficacy.
PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF PORTULACA QUADRIFIDA LINN.
Authors: SUPRIYA JAGTAP , KISHOR GUJAR , ANIKET GHARE 
Published: October 2019

Objective: The objective of the study was to investigate in vitro antimicrobial activity against enterotoxigenic Escherichia coli and Bacillus subtilis and preliminary phytochemical screening of the leaves of Portulaca quadrifida (Linn.).
Methods: The solvent extract such as petroleum ether, methanol, and water on the leaves of P. quadrifida (Linn) was prepared by Soxhlet extraction (continuous hot percolation method). These solvent extracts were screened for antimicrobial activity against enterotoxigenic E. coli and B. subtilis at various concentrations and were measured by observing zone of inhibition in mm by disc diffusion method (cup plate method).
Results: The preliminary phytochemical screening revealed the flavonoids, fats, and oils in all extracts. Similarly, the presence of alkaloids and tannins was obtained in the petroleum ether and methanolic extracts, while the presence of glycosides was obtained in the methanolic and water extracts. Further, proteins and sterols were found in petroleum extracts. The results of antimicrobial activity shown that methanolic extracts of the plant leaf showed good antimicrobial activity and petroleum ether and water extract showed similar activity but less antimicrobial activity than methanolic extract. The antimicrobial activities of extracts were compared with standard antibiotic such as chloramphenicol.
Conclusion: P. quadrifida (Linn.) has broad-spectrum antimicrobial activity and a potential source of new classes of antibiotics that could be useful for infectious disease chemotherapy and control. The phytochemical analysis of the crude extracts of this plant indicates the presence of major phytoconstituents which may have been responsible for the observed antimicrobial property.
EVALUATION OF HYPOLIPIDEMIC ACTIVITY OF FRUIT RIND EXTRACTS OF GARCINIA GUMMIGUTTA IN DIET-INDUCED HYPERLIPIDEMIC RATS
Authors: DATCHANAMURTY BARATHANE , K MANIMEKALAI , SALWE KARTIK J 
Published: October 2019

Objective: The objective of the study was to evaluate the hypolipidemic activity of fruit rind extracts of Garcinia gummi-gutta in diet-induced hyperlipidemic rats.
Methods: Hydroalcoholic extract of fruit rinds of G. gummi-gutta was prepared using Soxhlet apparatus. 30 Wistar albino rats were divided into five groups (n=6). Except for Group 1, all the other groups were treated with hyperlipidemic diet (HLD) for 4 weeks. After induction, the Groups 3 and 4 were treated with the extracts at 100 mg/kg and 200 mg/kg doses, respectively, whereas Group 5 received the standard drug atorvastatin at 10 mg/kg for the next 4 weeks. Lipid profile was estimated at the end of each week. At the end of the study, rats were sacrificed and the liver and heart were analyzed for histopathological changes. Data expressed as mean±standard error of the mean. Statistical analysis was done using one-way ANOVA followed by post hoc Tukey test. p<0.05 considered statistically significant.
Results: The serum levels of total cholesterol (TC), triglycerides (TG), very low-density lipoprotein (VLDL), and LDL were significantly increased and the levels of high-density lipoprotein (HDL) were significantly decreased when compared to normal control. The groups which received G. gummi-gutta rind extract at 100 mg/kg and 200 mg/kg showed a significant decrease in the mean cholesterol, TG, LDL, VLDL levels, and an increase in HDL levels when compared to the HLD group. The group which received the extract at higher dose (REGG200) was found better than the lower dose (REGG 100). REGG 200 showed significant improvement in increasing the HDL levels than all the test groups. The standard drug atorvastatin was found better than REGG100 but there was no significant difference between high doses of the extract and atorvastatin.
Conclusion: The fruit rinds extract of G. gummi-gutta was able to decrease the elevated serum lipids (TC, TG, LDL, and VLDL) and consequently increase the HDL levels in a dose-dependent manner.
STUDY ON MULTIPOTENT MEDICINAL ASPECTS OF SCHIMA WALLICHII (BARK) FROM NAGALAND, NE INDIA
Authors: BHATTACHARJEE MINAKSHI , SEMA Y VITOLU , PRATIMSARMA MANASH 
Published: October 2019

Objective: The present study focuses on the multipotent medicinal aspects of Schima wallichii bark.
Methods: The ethanol and water extracts of the plant were investigated its phytochemical, antibacterial, antioxidant, and anticoagulant as per the standard method.
Results: S. wallichii bark extract was effective against Staphylococcus aureus. It was also found to be a good antioxidant having an IC50 value of 98.7 in ethanolic extract. Further, the water extract increased in prothrombin time to 57.7 s against normal (12.3 s). The different phytochemicals, namely tannin, saponins, steroids, terpenoids, flavonoids, and glycosides, were present. Since the findings showed very less percent inhibition in comparison to curcumin-treated cells, thus both the extracts were both not effective in the antiproliferative assay.
Conclusion: We conclude that this plant is a good candidate for developing a natural drug.
SOLID DISPERSIONS: RESUSCITATING ORAL DELIVERY OF HYDROPHOBIC DRUGS
Authors: AGRAWAL RUCHI , RAZA ABID , PATEL OM PRAKASH 
Published: October 2019

Objective: This review article explores solid dispersions (SDs) as one of the suitable approaches to formulate poorly water-soluble drugs. The objective of this review on SD techniques is to explore their utility as a feasible, simple, and economically viable method for augmentation of dissolution of hydrophobic drugs.
Methods: Various types of SDs are classified and compared. Use of surfactants to stabilize the SDs and their potential advantages and disadvantages has been discussed. Different techniques for preparing and evaluating SDs are appraised along with discussions on scalability and industrial production. Review of the current research on SD along with future trends is also offered.
Results: Based on the various researches, SDs offer an efficient means of improving bioavailability while concurrently contributing to lower toxicity and dose-reduction.
Conclusion: Solid-dispersions have been and continue to be one of the key technologies for solving the issue of poor solubility for newer hydrophobic molecules which are being discovered. This would give a new lease of life for such drugs enabling them to be delivered in an effective way.
HYPOGLYCEMIC ACTIVITY OF A NOVEL POLYHERBAL FORMULATION IN STREPTOZOTOCIN-INDUCED DIABETIC RATS: A THERAPEUTIC STUDY
Authors: MAJUMDER PULAK , M PARIDHAVI 
Published: October 2019

Objective: The concept of the synergistic effect of poly-herbalism was as old as medicine history. Present novel polyherbal formulation (PHF) composed of five different herbs. The present investigation aimed to evaluate the synergistic therapeutic hypoglycemic potential of PHF against streptozotocin (STZ) (60 mg/kg b.w, ip)-induced diabetic rats.
Methods: For this therapeutic study, the dose was framed orally once a day to the test objects after STZ dosing at 500 mg/kg/5 ml dosage levels for 21 days. The transformation of body weight and blood glucose level was examined, and the histopathological changes of beta cells of the pancreas, cellular architectures of liver and kidney were also perceived after scarification of the objects.
Results: The outcomes were compared to that of glibenclamide (5 mg/kg) treated group. Declines of body weight and blood glucose levels were perceived in STZ-induced diabetic animals very significantly (p<0.01 or p<0.05). However, these diabetic changes were significantly (p<0.01 or p<0.05) decreased in PHF-dosing groups revealed more encouraging effects compared to that of glibenclamide. In the other hand, various liver function and enzymes test (creatinine, urea, total bilirubin, total albumin, alkaline phosphatase, gamma-glutamyl transferase, aspartate transaminases, and alanine transaminases) and lipid profile (total cholesterol, triglycerides, high-density lipoprotein cholesterol, total protein, low-density lipoprotein [LDL], and very LDL) studies strongly indicate the potential action of this novel formulation.
Conclusions: It is deliberated that PHF has the favorable effect to normalize the blood glucose levels, and also rejuvenation and reproduction of beta cells lead a better futuristic ant diabetic therapy for diabetic management.
EVALUATION OF PRO-APOPTOTIC EFFECTS OF β-MONOLINOLEIN ON METASTATIC BREAST CANCER CELL LINE MDA-MB-231
Authors: SOFI MOHAMMED SHAFI 
Published: October 2019

Objectives: The objective of this study was to evaluate the β-monolinolein as a potential therapy for breast cancer treatment.
Methods: The cytotoxic activity of β-monolinolein was evaluated by 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue exclusion assay. The cellular cytotoxicity and levels of cytosolic enzyme, lactate dehydrogenase (LDH), were measured by assessing μmoles of nicotinamide adenine dinucleotide/well/min. To confirm whether β-monolinolein induces apoptosis in 3,4-methylenedioxyamphetamine (MDA)- MB-231 cells, western blot and semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis were performed.
Results: For the 1st time, it was demonstrated that β-monolinolein strongly inhibits the growth of MDA-MB-231 cells, with an half maximal inhibitory concentration value of 12.5 μg/ml. <90% of cell death was achieved at higher concentrations after 48 h of treatment. Trypan blue assay showed that the cell viability was significantly decreased in a dose-dependent manner in MDA-MB-231 cells after 48 h of treatment. On the other hand, LDH activities in the cultured media were significantly elevated in a dose-dependent manner as compared to the control. Further, the western blot analysis showed that β-monolinolein leads to change in expression levels of important cell cycle regulators such as p21, Bax, Bcl-Xl, and Bcl-2 in MDA-MB-231 cells. The semiquantitative RT-PCR results indicated a significant upregulation of proapoptotic genes such as p53, p21, and Bax and downregulation of antiapoptotic gene Bcl-2.
Conclusion: These results indicate that β-monolinolein leads to change in expression of various cell cycle/apoptotic regulators and hence induces death in MDA-MB-231 cells.
STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF METFORMIN AND ERTUGLIFLOZIN BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH PDA DETECTION AND ITS APPLICATION TO TABLET DOSAGE FORM
Authors: JAGADEESH KADALI , N ANNAPURNA 
Published: October 2019

Objective: A sensitive, rapid, accurate, and precise stability indicating high-performance liquid chromatography method was developed and validated for the simultaneous estimation of metformin (MET) and ertugliflozin (ERT).
Methods: The planned chromatographic method was developed using Kromasil C18 column using 0.1 M sodium dihydrogen phosphate methanol (50:50, by volume, pH 4.0) as mobile phase system followed by peak area response measurement at 238 nm. The developed method was validated by means of ICH guidelines about system suitability, linearity, sensitivity, selectivity, accuracy, precision, robustness, and specificity.
Results: Calibration curves of MET and ERT are linear from 250 to 750 μg/ml and 3.75 to 11.25 μg/ml, respectively. Relative standard deviation is <2.0% and recovery is ̴ 100%. Successfully, the developed method was applied for the simultaneous determination of MET and ERT in tablets and to study degradation of MET and ERT in acidic, alkaline, oxidation, thermal, and photolytic degradation conditions. No obstructions from additional common excipients of tablets and degradants were observed.
Conclusion: The results recommended method suitability for the analysis of MET and ERT in quality control laboratories.
USING BREWER’S YEAST AND GINGER IN THE MANAGEMENT OF CONSTIPATION-PREDOMINANT IRRITABLE BOWEL SYNDROME: A RANDOMIZED DOUBLE-BLIND PLACEBO-CONTROLLED TRIAL
Authors: AL-JASSIM ZAINAB G 
Published: October 2019

Objective: The objective of the present study is to confirm and/or prove the beneficial outcomes from using Brewer’s yeast and Ginger in constipation-predominant irritable bowel syndrome (IBS-C) subjects compared to placebo.
Methods: A total of 45 patients suffering from IBS-C were enrolled in a double-blind placebo-controlled study as defined by Rome III criteria. Parallel groups were randomly assigned in this study: A placebo group, Brewer’s yeast group, and ginger group, taken daily for 20 days. IBS severity scale and visual analog scale for IBS (VAS-IBS) were used to assess the severity of pain, abdominal distention, and constipation (IBS-C) subjects. The data were measured at 3 times: At 0 time (T0), after 10 days of treatment (T10), and after 20 days of treatment (T20) for the three treatment groups.
Results: Intragroup analysis showed a clinically significant reduction in the symptoms of abdominal pain, distention, and constipation, in the Brewer’s yeast group compared to placebo after the 20 days of the study. There was also a significant reduction of abdominal distention and constipation symptoms in the ginger group throughout the study.
Conclusion: This study reveals the beneficial effects of Brewer’s yeast and ginger in reducing troublesome gastrointestinal symptoms in subjects with IBS-C and holds the promise to use them in IBS-C patient.