bluuebunny/crossref_metadata_2025_split huggingface dataset:
Astrocytic endothelin-1 overexpression promotes neural progenitor cells proliferation and differentiation into astrocytes via the Jak2/Stat3 pathway after stroke
Authors: Cheng Xiao , Yeung Patrick K. K. , Zhong Ke , Zilundu Prince L. M. , Zhou Lihua , Chung Sookja K. 
Published: November 2019

Abstract
Background
Endothelin-1 (ET-1) is synthesized and upregulated in astrocytes under stroke. We previously demonstrated that transgenic mice over-expressing astrocytic ET-1 (GET-1) displayed more severe neurological deficits characterized by a larger infarct after transient middle cerebral artery occlusion (tMCAO). ET-1 is a known vasoconstrictor, mitogenic, and a survival factor. However, it is unclear whether the observed severe brain damage in GET-1 mice post stroke is due to ET-1 dysregulation of neurogenesis by altering the stem cell niche.

Methods
Non-transgenic (Ntg) and GET-1 mice were subjected to tMCAO with 1 h occlusion followed by long-term reperfusion (from day 1 to day 28). Neurological function was assessed using a four-point scale method. Infarct area and volume were determined by 2,3,5-triphenyltetra-zolium chloride staining. Neural stem cell (NSC) proliferation and migration in subventricular zone (SVZ) were evaluated by immunofluorescence double labeling of bromodeoxyuridine (BrdU), Ki67 and Sox2, Nestin, and Doublecortin (DCX). NSC differentiation in SVZ was evaluated using the following immunofluorescence double immunostaining: BrdU and neuron-specific nuclear protein (NeuN), BrdU and glial fibrillary acidic protein (GFAP). Phospho-Stat3 (p-Stat3) expression detected by Western-blot and immunofluorescence staining.

Results
GET-1 mice displayed a more severe neurological deficit and larger infarct area after tMCAO injury. There was a significant increase of BrdU-labeled progenitor cell proliferation, which co-expressed with GFAP, at SVZ in the ipsilateral side of the GET-1 brain at 28 days after tMCAO. p-Stat3 expression was increased in both Ntg and GET-1 mice in the ischemia brain at 7 days after tMCAO. p-Stat3 expression was significantly upregulated in the ipsilateral side in the GET-1 brain than that in the Ntg brain at 7 days after tMCAO. Furthermore, GET-1 mice treated with AG490 (a JAK2/Stat3 inhibitor) sh owed a significant reduction in neurological deficit along with reduced infarct area and dwarfed astrocytic differentiation in the ipsilateral brain after tMCAO.

Conclusions
The data indicate that astrocytic endothelin-1 overexpression promotes progenitor stem cell proliferation and astr ocytic differentiation via the Jak2/Stat3 pathway.
Kidney transplant outcomes in HIV-positive patients: a systematic review and meta-analysis
Authors: Zheng Xin , Gong Lian , Xue Wenrui , Zeng Song , Xu Yue , Zhang Yu , Hu Xiaopeng 
Published: November 2019

Abstract
Background
Kidney transplantation is now a viable alternative to dialysis in HIV-positive patients who achieve good immunovirological control with the currently available antiretroviral therapy regimens. This systematic review and meta-analysis investigate the published evidence of outcome and risk of kidney transplantation in HIV-positive patients following the PRISMA guidelines.

Methods
Searches of PubMed, the Cochrane Library and EMBASE identified 27 cohort studies and 1670 case series evaluating the survival of HIV-positive kidney transplant patients published between July 2003 and May 2018. The regimens for induction, maintenance therapy and highly active antiretroviral therapy, acute rejection, patient and graft survival, CD4 count and infectious complications were recorded. We evaluated the patient survival and graft survival at 1 and 3 years respectively, acute rejection rate and also other infectious complications by using a random-effects analysis.

Results
At 1 year, patient survival was 0.97 (95% CI 0.95; 0.98), graft survival was 0.91 (95% CI 0.88; 0.94), acute rejection was 0.33 (95% CI 0.28; 0.38), and infectious complications was 0.41 (95% CI 0.34; 0.50), and at 3 years, patient survival was 0.94 (95% CI 0.90; 0.97) and graft survival was 0.81 (95% CI 0.74; 0.87).

Conclusions
With careful selection and evaluation, kidney transplantation can be performed with good outcomes in HIV-positive patients.
Sensory assessment of Cercospora beticola sporulation for phenotyping the partial disease resistance of sugar beet genotypes
Authors: Oerke Erich-Christian , Leucker Marlene , Steiner Ulrike 
Published: November 2019

Abstract
Background
Due to its high damaging potential, Cercospora leaf spot (CLS) caused by Cercospora beticola is a continuous threat to sugar beet production worldwide. Breeding for disease resistance is hampered by the quantitative nature of resistance which may result from differences in penetration, colonization, and sporulation of the pathogen on sugar beet genotypes. In particular, problems in the quantitative assessment of C. beticola sporulation have resulted in the common practice to assess field resistance late in the growth period as quantitative resistance parameter. Recently, hyperspectral sensors have shown potential to assess differences in CLS severity. Hyperspectral microscopy was used for the quantification of C. beticola sporulation on sugar beet leaves in order to characterize the host plant suitability / resistance of genotypes for decision-making in breeding for CLS resistance.

Results
Assays with attached and detached leaves demonstrated that vital plant tissue is essential for the full potential of genotypic mechanisms of disease resistance and susceptibility. Spectral information (400 to 900 nm, 160 wavebands) of CLSs recorded before and after induction of C. beticola sporulation allowed the identification of sporulating leaf spot sub-areas. A supervised classification and quantification of sporulation structures was possible, but the necessity of genotype-specific reference spectra restricts the general applicability of this approach. Fungal sporulation could be quantified independent of the host plant genotype by calculating the area under the difference reflection spectrum from hyperspectral imaging before and with sporulation. The overall relationship between sensor-based and visual quantification of C. beticola sporulation on five genotypes differing in CLS resistance was R2 = 0.81; count-based differences among genotypes could be reproduced spectrally.

Conclusions
For the first time, hyperspectral imaging was successfully tested for the quantification of sporulation as a fungal activity depending on host plant suitability. The potential of this non-invasive and non-destructive approach for the quantification of fungal sporulation in other host–pathogen systems and for the phenotyping of crop traits complex as sporulation resistance is discussed.
A child with a novel ACAN missense variant mimicking a septic arthritis
Authors: Florio Angelo , Papa Riccardo , Caorsi Roberta , Consolaro Alessandro , Gastaldi Roberto , Gattorno Marco , Picco Paolo 
Published: November 2019

AbstractHeterozygous mutations of the ACAN gene have been associated with a broad spectrum of non-lethal skeletal dysplasias, called Aggrecanopathies. We report a case of a child with severe inflammatory elbow involvement mimicking septic arthritis who carried the new ACAN missense variant c.6970 T > C, p.Trp2324Arg. The comprehensive clinical evaluation of the patient and his family, focused on the associated clinical features (facial dysmorphisms, short stature, brachydactily), led us to suspect a hereditary condition. Our findings suggest that Aggrecanopathies should be considered in children with familial short stature, poor growth spurt and joint involvement.
Structural variant calling: the long and the short of it
Authors: Mahmoud Medhat , Gobet Nastassia , Cruz-Dávalos Diana Ivette , Mounier Ninon , Dessimoz Christophe , Sedlazeck Fritz J. 
Published: November 2019

AbstractRecent research into structural variants (SVs) has established their importance to medicine and molecular biology, elucidating their role in various diseases, regulation of gene expression, ethnic diversity, and large-scale chromosome evolution—giving rise to the differences within populations and among species. Nevertheless, characterizing SVs and determining the optimal approach for a given experimental design remains a computational and scientific challenge. Multiple approaches have emerged to target various SV classes, zygosities, and size ranges. Here, we review these approaches with respect to their ability to infer SVs across the full spectrum of large, complex variations and present computational methods for each approach.
DOD-ETL: distributed on-demand ETL for near real-time business intelligence
Authors: Machado Gustavo V. , Cunha Ítalo , Pereira Adriano C. M. , Oliveira Leonardo B. 
Published: November 2019

AbstractThe competitive dynamics of the globalized market demand information on the internal and external reality of corporations. Information is a precious asset and is responsible for establishing key advantages to enable companies to maintain their leadership. However, reliable, rich information is no longer the only goal. The time frame to extract information from data determines its usefulness. This work proposes DOD-ETL, a tool that addresses, in an innovative manner, the main bottleneck in Business Intelligence solutions, the Extract Transform Load process (ETL), providing it in near real-time. DOD-ETL achieves this by combining an on-demand data stream pipeline with a distributed, parallel and technology-independent architecture with in-memory caching and efficient data partitioning. We compared DOD-ETL with other Stream Processing frameworks used to perform near real-time ETL and found DOD-ETL executes workloads up to 10 times faster. We have deployed it in a large steelworks as a replacement for its previous ETL solution, enabling near real-time reports previously unavailable.
Electrical transient modeling for appliance characterization
Authors: Nait-Meziane Mohamed , Ravier Philippe , Abed-Meraim Karim , Lamarque Guy , Bunetel Jean-Charles Le , Raingeaud Yves 
Published: November 2019

AbstractTransient signals are characteristic of the underlying phenomenon generating them, which makes their analysis useful in many fields. Transients occur as a sudden change between two steady state regimes, subsist for a short period, and tend to decay over time. Hence, superimposed damped sinusoids (SDS) were extensively used for transients modeling as they are adequate for describing decaying phenomena. However, SDS are not adapted for modeling the turn-on transient current of electrical appliances as it tends to decay to a steady state that is different from the one preceding it. In this paper, we propose a new and more suitable model for these signals for the purpose of characterizing appliances. We also propose an algorithm for the model parameter estimation and validate its performance on simulated and real data. Moreover, we give an example on the use of the model parameters as features for the classification of appliances using the Controlled On/Off Loads Library (COOLL) dataset. The results show that the proposed algorithm is efficient and that for real data the network fundamental frequency must be estimated to account for its variations around the nominal value. Finally, real data experiments showed that the model parameters used as features yielded a classification accuracy of 98%.
Second-order control of complex systems with correlated synthetic data
Authors: Raimbault Juste 
Published: November 2019

AbstractThe generation of synthetic data is an essential tool to study complex systems, allowing for example to test models of these in precisely controlled settings, or to parametrize simulation models when data is missing. This paper focuses on the generation of synthetic data with an emphasis on correlation structure. We introduce a new methodology to generate such correlated synthetic data. It is implemented in the field of socio-spatial systems, more precisely by coupling an urban growth model with a transportation network generation model. We also show the genericity of the method with an application on financial time-series. The simulation results show that the generation of correlated synthetic data for such systems is indeed feasible within a broad range of correlations, and suggest applications of such synthetic datasets.
Mapping actionable pathways and mutations in brain tumours using targeted RNA next generation sequencing
Authors: Lenting Krissie , van den Heuvel Corina N. A. M. , van Ewijk Anne , ElMelik Duaa , de Boer Remco , Tindall Elizabeth , Wei Ge , Kusters Benno , te Dorsthorst Maarten , ter Laan Mark , Huynen Martijn A. , Leenders William P. 
Published: November 2019

AbstractMany biology-based precision drugs are available that neutralize aberrant molecular pathways in cancer. Molecular heterogeneity and the lack of reliable companion diagnostic biomarkers for many drugs makes targeted treatment of cancer inaccurate for many individuals. Identifying actionable hyperactive biological pathways in individual cancers may improve this situation.To achieve this we applied a novel targeted RNA next generation sequencing (t/RNA-NGS) technique to surgically obtained glioma tissues. The test combines mutation detection with analysis of biological pathway activities that are involved in tumour behavior in many cancer types (e.g. tyrosine kinase signaling, angiogenesis signaling, immune response, metabolism), via quantitative measurement of transcript levels and splice variants of hundreds of genes. We here present proof of concept that the technique, which uses molecular inversion probes, generates a histology-independent molecular diagnosis and identifies classifiers that are strongly associated with conventional histopathology diagnoses and even with patient prognosis. The test not only confirmed known glioma-associated molecular aberrations but also identified aberrant expression levels of actionable genes and mutations that have so far been considered not to be associated with glioma, opening up the possibility of drug repurposing for individual patients. Its cost-effectiveness makes t/RNA-NGS to an attractive instrument to aid oncologists in therapy decision making.
Gemcitabine-incorporated polyurethane films for controlled release of an anticancer drug
Authors: Choi Seong Hoon , Cho Il-Hoon , Park Sangsoo 
Published: November 2019

Abstract

Background
Local delivery of anti-cancer drugs through a stent is a very promising and anticipated treatment modality for patients who have obstructions in their gastrointestinal tract with malignant tumors. Anticancer drug release via stents, however, needs to be optimized with respect to drug delivery behavior for the stents to be effective for prolonged containment of tumor proliferation and stent re-obstruction. Local stent-based drug delivery has been tested using an effective anti-cancer drug, gemcitabine, but the release from the stent-coated polyurethane films is often too fast and the drug is depleted from the coated film virtually in a day.

Methods
To moderate the drug release from a polyurethane film, a gemcitabine-incorporated polyurethane film was enveloped with a pure polyurethane film, with no drug loading, and with a silicone film by solution casting after activation of the silicone film surface with plasma treatment.

Results
The pure polyurethane barrier film was effective; the interface of the two were indistinguishable on scanning electron microscopy, and the initial burst, i.e., the cumulative release in a day, decreased from 90 to 26%. The silicone film barrier, on the other hand, was defective as voids were seen using a scanning electron microscope, and micro-separation of the two layers was observed after the film was immersed in phosphate-buffered saline for 1 day during the in vitro drug release study.

Conclusions
Enveloping a gemcitabine-releasing polyurethane film with a homo-polymer barrier film was quite effective for moderating the initial burst of gemcitabine, thus, prolonging the release time of the drug. Enveloping the polyurethane film with a silicone film was also possible after plasma treatment of the silicone film surface, but the two films eventually separated in the aqueous environment. More studies are needed to tune the drug release behavior of gemcitabine from the stent covering film before attempting a clinical application of an anti-cancer drug releasing stent.
Barrier bucket and transversely split beams for loss-free multi-turn extraction in synchrotrons
Authors: Vadai M. , Alomainy A. , Damerau H. , Gilardoni S. , Giovannozzi M. , Huschauer A. 
Published: November 2019

Abstract
A special radio-frequency manipulation has been studied and successfully implemented at the CERN Proton Synchrotron for the first time. This technique aims at depleting a well-defined fraction of a continuous longitudinal beam distribution by creating a so-called barrier bucket. In addition to the intrinsic interest for its originality, this approach has an immediate application at CERN in combination with the Multi-Turn Extraction scheme. The combination of these two exotic techniques into a single, highly sophisticated process allows to dramatically reduce the beam loss at PS extraction, thus opening the door to the production of high-intensity proton beams for future fixed-target experiments at the CERN Super Proton Synchrotron.
Dynamics of activating and repressive histone modifications in Drosophila neural stem cell lineages and brain tumors
Authors: Abdusselamoglu Merve Deniz , Landskron Lisa , Bowman Sarah K. , Eroglu Elif , Burkard Thomas , Kingston Robert E. , Knoblich Jürgen A. 
Published: November 2019

ABSTRACT
During central nervous system development, spatiotemporal gene expression programs mediate specific lineage decisions to generate neuronal and glial cell types from neural stem cells (NSCs). However, little is known about the epigenetic landscape underlying these highly complex developmental events. Here, we perform ChIP-seq on distinct subtypes of Drosophila FACS-purified NSCs and their differentiated progeny to dissect the epigenetic changes accompanying the major lineage decisions in vivo. By analyzing active and repressive histone modifications, we show that stem cell identity genes are silenced during differentiation by loss of their activating marks and not via repressive histone modifications. Our analysis also uncovers a new set of genes specifically required for altering lineage patterns in type II neuroblasts (NBs), one of the two main Drosophila NSC identities. Finally, we demonstrate that this subtype specification in NBs, unlike NSC differentiation, requires Polycomb-group-mediated repression.
Antibiotics for Aspiration Pneumonia in Neurologically Impaired Children
Authors: Thomson Joanna , Hall Matt , Ambroggio Lilliam , Berry Jay G , Stone Bryan , Srivastava Rajendu , Shah Samir S 
Published: November 2019

OBJECTIVETo compare hospital outcomes associated with commonly used antibiotic therapies for aspiration pneumonia in children with neurologic impairment (NI).DESIGN/METHODSA retrospective study of children with NI hospitalized with aspiration pneumonia at 39 children's hospitals in the Pediatric Health Information System database. Exposure was empiric antibiotic therapy classified by antimicrobial activity. Outcomes included acute respiratory failure, intensive care unit (ICU) transfer, and hospital length of stay (LOS). Multivariable regression evaluated associations between exposure and outcomes and adjusted for confounders, including medical complexity and acute illness severity.RESULTSIn the adjusted analysis, children receiving Gram‐negative coverage alone had two‐fold greater odds of respiratory failure (odds ratio [OR] 2.15; 95% CI: 1.41‐3.27), greater odds of ICU transfer (OR 1.80; 95% CI: 1.03‐3.14), and longer LOS [adjusted rate ratio (RR) 1.28; 95% CI: 1.16‐1.41] than those receiving anaerobic coverage alone. Children receiving anaerobic and Gram‐negative coverage had higher odds of respiratory failure (OR 1.65; 95% CI: 1.19‐2.28) than those receiving anaerobic coverage alone, but ICU transfer (OR 1.15; 95% CI: 0.73‐1.80) and length of stay (RR 1.07; 95% CI: 0.98‐1.16) did not statistically differ. For children receiving anaerobic, Gram‐negative, and P. aeruginosa coverage, LOS was shorter (RR 0.83; 95% CI: 0.76‐0.90) than those receiving anaerobic coverage alone; odds of respiratory failure and ICU transfer rates did not significantly differ.CONCLUSIONSAnaerobic therapy appears to be important in the treatment of aspiration pneumonia in children with NI. While Gram‐negative coverage alone was associated with worse outcomes, its addition to anaerobic therapy may not yield improved outcomes.
Il romanzo digitale:
Authors: Italia Paola 
Published: November 2019

L’articolo propone una riflessione sullo stato dell’arte del romanzo italiano nel mondo digi- tale svolta attraverso l’analisi dei testi in prosa della modernità presenti nel portale Biblio- teca Italiana e affronta i casi di studio di Manzoni, Pirandello e Tozzi, per analizzare tre progetti complementari e diversi di edizione cartacea e digitale, e di interazione tra ricerca accademica, istituzioni bibliotecarie ed editoria. I casi mostrano come il romanzo, per la sua polifonia, le sue dinamiche genetiche, la sua contaminazione con le arti visive e drammati- che, richieda una infrastruttura digitale che metta in relazione tutti questi aspetti tra loro, anche in chiave didattica, costituendo un banco di prova della necessaria trasformazione dell’edizione critica digitale in un sito di ricerca e conoscenza dei testi e dei loro autori.
This article proposes a reflection on the state of the art of the Italian novel in the digital world carried out through the analysis of modern prose texts in the website BibliotecaItaliana.it, examining the case studies of Manzoni, Pirandello, and Tozzi, and in particular three com- plementary projects and several digital editions as well as interactions among academic rese- arch, institutions, librarians, and publishers. The case studies show how the novel — thanks especially to its polyphony, its genetic dynamics and contamination with the visual and dra- matic arts — requires an infrastructure that also links all these aspects from an educational point of view. The novel may be considered as a testing ground for the necessary transforma- tion of the digital critical edition into a “knowledge site” of texts and their authors.
A design and evaluation framework for digital health interventions
Authors: Kowatsch Tobias , Otto Lena , Harperink Samira , Cotti Amanda , Schlieter Hannes 
Published: November 2019

Abstract
Digital health interventions (DHIs) have the potential to help the growing number of chronic disease patients better manage their everyday lives. However, guidelines for the systematic development of DHIs are still scarce. The current work has, therefore, the objective to propose a framework for the design and evaluation of DHIs (DEDHI). The DEDHI framework is meant to support both researchers and practitioners alike from early conceptual DHI models to large-scale implementations of DHIs in the healthcare market.
On nonlinear neutral Liouville–Caputo-type fractional differential equations with Riemann–Liouville integral boundary conditions
Authors: Ahmad Bashir , Ntouyas Sotiris K. , Alsaedi Ahmed 
Published: November 2019

AbstractThis paper studies neutral Liouville–Caputo-type fractional differential equations and inclusions supplemented with nonlocal Riemann–Liouville-type integral boundary conditions. Sadovskii’s fixed point theorem is applied to establish the existence result for the single-valued case, while the multivalued case is investigated by using nonlinear alternative for contractive maps. Examples are constructed to illustrate the main results. The case of nonlinear nonlocal boundary conditions is also discussed.