bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphorothioate Modification of mRNA Accelerates the Rate of Translation Initiation to Provide More Efficient Protein Synthesis
Authors: Kawaguchi Daisuke , Kodama Ayumi , Abe Naoko , Takebuchi Kei , Hashiya Fumitaka , Tomoike Fumiaki , Nakamoto Kosuke , Kimura Yasuaki , Shimizu Yoshihiro , Abe Hiroshi 
Published: July 2020

AbstractMessenger RNAs (mRNAs) with phosphorothioate modification (PS‐mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared, and the translation reaction was evaluated using an E. coli cell‐free translation system. Protein synthesis from PS‐mRNA increased in 12 of 15 patterns when compared with that of unmodified mRNA. The protein yield increased 22‐fold when the phosphorothioate modification at A/C sites was introduced into the region from the 5′‐end to the initiation codon. Single‐turnover analysis of PS‐mRNA translation showed that phosphorothioate modification increases the number of translating ribosomes, thus suggesting that the rate of translation initiation (rate of ribosome complex formation) is positively affected by the modification. The method provides a new strategy for improving translation by using non‐natural mRNA.
Pressure‐Suppressed Carrier Trapping Leads to Enhanced Emission in Two‐Dimensional Perovskite (HA)2(GA)Pb2I7
Authors: Guo Songhao , Zhao Yongsheng , Bu Kejun , Fu Yongping , Luo Hui , Chen Mengting , Hautzinger Matthew P. , Wang Yingqi , Jin Song , Yang Wenge , Lü Xujie 
Published: July 2020

AbstractA remarkable PL enhancement by 12 fold is achieved using pressure to modulate the structure of a recently developed 2D perovskite (HA)2(GA)Pb2I7 (HA=n‐hexylammonium, GA=guanidinium). This structure features a previously unattainable, extremely large cage. In situ structural, spectroscopic, and theoretical analyses reveal that lattice compression under a mild pressure within 1.6 GPa considerably suppresses the carrier trapping, leading to significantly enhanced emission. Further pressurization induces a non‐luminescent amorphous yellow phase, which is retained and exhibits a continuously increasing band gap during decompression. When the pressure is released to 1.5 GPa, emission can be triggered by above‐band gap laser irradiation, accompanied by a color change from yellow to orange. The obtained orange phase could be retained at ambient conditions and exhibits two‐fold higher PL emission compared with the pristine (HA)2(GA)Pb2I7.
Inverse Vulcanization of Styrylethyltrimethoxysilane–Coated Surfaces, Particles, and Crosslinked Materials
Authors: Scheiger Johannes M. , Direksilp Chatrawee , Falkenstein Patricia , Welle Alexander , Koenig Meike , Heissler Stefan , Matysik Jörg , Levkin Pavel A. , Theato Patrick 
Published: July 2020

AbstractSulfur as a side product of natural gas and oil refining is an underused resource. Converting landfilled sulfur waste into materials merges the ecological imperative of resource efficiency with economic considerations. A strategy to convert sulfur into polymeric materials is the inverse vulcanization reaction of sulfur with alkenes. However, the materials formed are of limited applicability, because they need to be cured at high temperatures (>130 °C) for many hours. Herein, we report the reaction of elemental sulfur with styrylethyltrimethoxysilane. Marrying the inverse vulcanization and silane chemistry yielded high sulfur content polysilanes, which could be cured via room temperature polycondensation to obtain coated surfaces, particles, and crosslinked materials. The polycondensation was triggered by hydrolysis of poly(sulfur‐r‐styrylethyltrimethoxysilane) (poly(Sn‐r‐StyTMS) under mild conditions (HCl, pH 4). For the first time, an inverse vulcanization polymer could be conveniently coated and mildly cured via post‐polycondensation. Silica microparticles coated with the high sulfur content polymer could improve their Hg2+ ion remediation capability.
Altered Properties of Endothelial Cells and Mesenchymal Stem Cells Underlying the Development of Scleroderma‐like Vasculopathy in KLF5+/−;Fli‐1+/− Mice
Authors: Nakamura Kouki , Taniguchi Takashi , Hirabayashi Megumi , Yamashita Takashi , Saigusa Ryosuke , Miura Shunsuke , Takahashi Takehiro , Toyama Tetsuo , Ichimura Yohei , Yoshizaki Ayumi , Trojanowska Maria , Fujiu Katsuhito , Nagai Ryozo , Sato Shinichi , Asano Yoshihide 
Published: July 2020

ObjectiveIn prevous studies, we established a new animal model, KLF5+/−;Fli‐1+/− mice, in which fundamental pathologic features of systemic sclerosis (SSc) are broadly recapitulated. SSc vasculopathy is believed to occur as a result of impaired vascular remodeling, but its detailed mechanism of action remains unknown. To address this, the present study investigated the properties of dermal microvascular endothelial cells (DMECs), bone marrow–derived endothelial progenitor cells (BM‐EPCs), and bone marrow–derived mesenchymal stem cells (BM‐MSCs), a precursor of pericytes, in KLF5+/−;Fli‐1+/− mice.MethodsNeovascularization and angiogenesis were assessed in KLF5+/−;Fli‐1+/− mice by in vivo Matrigel plug assay and in vitro tube formation assay, respectively. The properties of mouse BM‐EPCs and BM‐MSCs were assessed with in vitro studies. Dermal vasculature was visualized in vivo by injecting the mice with fluorescein isothiocyanate–conjugated dextran.ResultsNeovascularization was diminished in skin‐embedded Matrigel plugs from KLF5+/−;Fli‐1+/− mice. DMECs from KLF5+/−;Fli‐1+/− mice showed defective tubulogenic activity, decreased expression of VE‐cadherin and CD31, and an imbalance in the expression of Notch1/Dll4, suggesting that angiogenesis and anastomosis are disturbed. KLF5+/−;Fli‐1+/− mouse BM‐MSCs exhibited enhanced proliferation and migration and increased collagen production following stimulation with transforming growth factor β1, indicating that these cells differentiate preferentially into myofibroblasts rather than pericytes. KLF5+/−;Fli‐1+/− mouse BM‐EPCs displayed a transition toward mesenchymal cells, suggesting that vasculogenesis is impaired. Wound healing was delayed in KLF5+/−;Fli‐1+/− mice (mean ± SD healing time 15.67 ± 0.82 days versus 13.50 ± 0.84 days; P = 0.0017), and the vascular network was poorly developed in wound scar tissue.ConclusionThe characteristics observed in the KLF5+/−;Fli‐1+/− mouse model — specifically, impaired neovascularization and vascular maturation — are similar to those observed in human SSc, and could be at least partially attributable to the induction of SSc‐like properties in DMECs, BM‐EPCs, and BM‐MSCs. These findings indicate the critical contribution of Klf5 and Fli1 deficiency in vascular cells and related cell precursors to the development of SSc vasculopathy.
Catalytic Nitrile Hydroboration: A Route to N,N‐Diborylamines and Uses Thereof
Authors: Hayrapetyan Davit , Khalimon Andrey Y. 
Published: July 2020

AbstractCatalytic reduction of nitriles is considered as an attractive and atom‐economical route to a diversity of synthetically valuable primary amines. Compared to other methods, dihydroboration approach has been developed relatively recently but has already attracted the attention of many research groups due to reasonably mild reaction conditions, selectivity control and the access to N,N‐diborylamines, which emerged as powerful reagents for C−N bond forming reactions. Early developments in catalytic dihydroboration of nitriles implied precious metal catalysts along with harsh conditions and prolonged reaction times, whereas recent advances mostly rely on base and main group metal catalytic systems with significantly improved profiles. This minireview aims to provide an overview of advances and challenges of dihydroboration of nitriles with d‐, f‐ and main group metal catalysts. Mechanistic features of different catalytic systems, functional group tolerance and scope of the methods are also presented. The synthetic utility of N,N‐diborylamies, beyond simple protodeborylation, is discussed in the aspect of N‐arylation, imine and amide synthesis.
Observer‐based adaptive second‐order non‐singular fast terminal sliding mode controller for robotic manipulators
Authors: Liu Haitao , Sun Jian , Nie Jianhao , Zou Lanping 
Published: July 2020

AbstractIn this paper, a second‐order non‐singular fast terminal sliding mode controller is proposed for robotic manipulators in the presence of uncertainties and disturbances. Adaptive control is used to obtain robustness to system uncertainties and disturbances. The improved high‐gain observer is designed to estimate the speed information, which makes the controller more applicable in practice. Theorem proof and simulations demonstrate the effectiveness of the proposed controller.
Silica nanoparticle assists determining liver cancer gene sequence on interdigitated electrode surface
Authors: Song Feifei , Yang Yi , Gopinath Subash C.B. 
Published: July 2020

AbstractA high‐performance interdigitated electrode (IDE) biosensing surface was reported here by utilizing self‐assembled silica nanoparticle (SiNP). The modified surface was used to evaluate the complementation of hairpin forming region from Mitoxantrone resistance gene 7 (MXR7; liver cancer‐related short gene). The conjugated SiNPs on 3‐aminopropyl triethoxysilane functionalization were captured with probe sequence on IDE biosensing surface. The physical and chemically modified surface was used to quantify MXR7 and an increment in the current response upon complementation was noticed. Limit of target DNA detection was calculated (1–10 fM) and this label‐free detection is at the comparable level to the fluorescent‐based sensing. A linear regression was calculated [y = 0.243x − 0.0773; R² = 0.9336] and the sensitivity was 1 fM on the linear range of 1 fM to 10 pM. With the strong attachment of capture DNA on IDE through SiNP, the surface clearly discriminates the specificity (complementary) versus nonspecificity (complete‐, single‐, and triple‐mismatched sequences). This detection strategy helps to determine liver cancer progression and the similar strategy can be followed for other gene sequence complementation.
Factors influencing the application of nature as inspiration for sustainability‐oriented innovation in multinational corporations
Authors: Mead Taryn , Jeanrenaud Sally , Bessant John 
Published: July 2020

AbstractNature‐inspired innovation (NII) is an increasingly common innovation strategy, yet little is known about its implementation within corporate innovation settings. This paper asks what factors influence the application of NII in corporate contexts. It combines theoretical perspectives from innovation adoption and sustainability‐oriented innovation to explore factors that influence the implementation of NII. Six case studies of multinational corporations applying NII were analyzed, using data collected through semistructured interviews (N = 45), a review of internal project documents, and publicly available materials. Results indicate that successful implementation depends upon the characteristics of the innovation context, decision‐making units, and the innovation itself, such as an advanced organizational progression of corporate sustainability, senior leadership support of NII, design expertise within innovation teams, flexible innovation management processes, and collaboration with outside enabling stakeholders. NII should be viewed as a longer‐term investment in developing organizational sustainability culture rather than a one‐off approach to innovation.
Can environmental investments benefit environmental performance? The moderating roles of institutional environment and foreign direct investment
Authors: Li Ruiqian , Ramanathan Ramakrishnan 
Published: July 2020

AbstractThe contribution of environmental investments (EIs) to environmental performance (EP) is a lively topic for environmental researchers across the world. In spite of huge amount of research, there is still lack of clarity on the moderating factors that affect the role played by EI. In this study, we distinguish EI into pollution control investments (PCIs) and pollution prevention investments (PPIs). We further investigate whether institutional environment and foreign direct investment (FDI) can play their moderating effects both on the relationship between EI and EP and on the relationships between different types of investments and EP or not. The results indicate that EI has a positive effect on EP. More specifically, PPI plays a stronger positive role in EP, but PCI does not have a significant effect on EP. In addition, both institutional environment and FDI can strengthen the positive impact of EI on EP. The increase of EI in regions with better institutional environment or high FDI can lead to greater improvement in EP. These moderating effects of institutional environment and FDI are also confirmed on the link between PPI and EP. In summary, our results reinforce the existing views that EI, and specifically PPI, can improve EP but further contribute to the understanding of the positive moderating roles played by the institutional environment and FDI on the link between EI and EP.
Retracted: Circ‐SPECC1 modulates TGFβ2 and autophagy under oxidative stress by sponging miR‐33a to promote hepatocellular carcinoma tumorigenesis
Authors: Zhang Bin , Liu Zhiyi , Cao Kuan , Shan Wengang , Liu Jin , Wen Quan , Wang Renhao 
Published: July 2020

AbstractCircular RNAs (circRNAs) play vital roles in the pathogenesis and development of multiple cancers, including hepatocellular carcinoma (HCC). Nevertheless, the regulatory mechanisms of circ‐SPECC1 in HCC remain poorly understood. In our study, we found that circ‐SPECC1 was apparently downregulated in H2O2‐treated HCC cells. Additionally, knockdown of circ‐SPECC1 inhibited cell proliferation and promoted cell apoptosis of HCC cells under H2O2 treatment. Moreover, circ‐SPECC1 inhibited miR‐33a expression by direct interaction, and miR‐33a inhibitor partially reversed the effect of circ‐SPECC1 knockdown on proliferation and apoptosis of H2O2‐treated HCC cells. Furthermore, TGFβ2 was demonstrated to be a target gene of miR‐33a and TGFβ2 overexpression rescued the phenotypes of HCC cells attenuated by miR‐33a mimics. Meanwhile, autophagy inhibition by 3‐methyladenine (3‐MA) abrogated the effect of miR‐33a mimics on proliferation and apoptosis of H2O2‐treated HCC cells. Finally, knockdown of circ‐SPECC1 hindered tumor growth in vivo. In conclusion, our study demonstrated that circ‐SPECC1 regulated TGFβ2 and autophagy to promote HCC tumorigenesis under oxidative stress via miR‐33a. These findings might provide potential treatment strategies for patients with HCC.
The impact of bone marrow fibrosis and JAK2 expression on clinical outcomes in patients with newly diagnosed multiple myeloma treated with immunomodulatory agents and/or proteasome inhibitors
Authors: Paul Barry , Zhao Yue , Loitsch Gavin , Feinberg Daniel , Mathews Parker , Barak Ian , Dupuis Megan , Li Zhiguo , Rein Lindsay , Wang Endi , Kang Yubin 
Published: July 2020

AbstractWe determined the impact of bone marrow fibrosis (BMF) on the clinical outcomes of newly diagnosed multiple myeloma (NDMM) patients in the current era of myeloma therapy. A total of 393 MM patients were included in the final analysis. The median followup was 83 months (range: 3.9 to 212 months). BMF was noted in 122 (48.2%) evaluable patients. Median progression free survival (PFS) in patients without BMF was 30.2 (95% CI: 24.7‐38.0) months, and 21.1 (95% CI: 18.8‐27.5) months in patients with BMF present (P = .024). Median overall survival (OS) was 61.2 (95% CI: 51.5‐81.2) months in patients without BMF, and 45.1 (95% CI: 38.7‐57.0) months in patients with BMF (P = .0048). A subset of 99 patients had their bone marrow biopsies stained for JAK1 and JAK2 by immunohistochemistry. Of these samples 67 (67.7%) patients had detectable JAK2 expression predominantly noted on bone marrow megakaryocytes. JAK2 expression correlated with myeloma disease stage (P = .0071). Our study represents the largest dataset to date examining the association of BMF with prognosis in the era of novel therapies and widespread use of hematopoietic stem cell transplant (HSCT). Our data suggest that MM patients with BMF (particularly those with extensive BMF) have a poorer prognosis even when treated with immunomodulatory agents and proteasome inhibitors.
Genome‐wide DNA methylome analysis reveals methylation subtypes with different clinical outcomes for acute myeloid leukemia patients
Authors: Gao Haiyan , He Xin , Li Qiang , Wang Ying , Tian Yaoyao , Chen Xi , Wang Jinghua , Guo Yan , Wang Wei , Li Xiaoyun 
Published: July 2020

AbstractLeukemia is the second common blood cancer after lymphoma, and its incidence rate has an increasing trend in recent years. Acute myeloid leukemia (AML) is one of the prevalent forms of leukemia. Although previous studies have investigated the methylation profile for AML patients, the AML methylation subtypes based on the genome‐wide methylome are still unclear. In the present study, we identified three methylation subtypes for AML samples based on the methylation profiles at CGI, CGI shore, CGI shelf, and opensea genomic contexts. Analyzing the molecular characteristics and clinical factors of the three subtypes revealed different methylation patterns and clinical outcomes between them. Further analysis revealed subtype dependent marker genes and their promoter CpG sites with regulatory function. Finally, we found that combining the AML patient age and methylation pattern brought better clinical outcome classification. In conclusion, we identified AML methylation subtypes and their marker genes, these results may help to excavate potential targets for clinical therapy and the development of precision medicine for AML patients.
MicroRNA‐92b acts as an oncogene by targeting PTEN/AKT in NSCLC
Authors: Guo Jia‐Hui , Fang Hai‐Yun , Yang Jun‐Mei , Liu Shan‐Ling , Yao Qiang‐Hua , Fan Yi‐Juan , Zhao Mei , Liu Feng , Zhang Quan‐Wu , Gao Feng‐Hou 
Published: July 2020

MicroRNAs can act as tumour suppressors or oncogenes by regulating cellular differentiation, proliferation and apoptosis, and the dysregulation of miRNA is involved in the occurrence and development of NSCLC. Here, we provided evidence that miR‐92b as an oncogene in NSCLC by targeting PTEN/AKT. We found that miR‐92b was up‐regulated in human NSCLC tissues and cell lines. MiR‐92b knockdown suppressed the NSCLC cells proliferation and migration in both in vivo and in vitro models. Conversely, miR‐92b overexpression induced an aggressive phenotype. Moreover, miR‐92b‐mediated regulation of NSCLC cell proliferation and migration depended on binding to PTEN mRNA, which then led to the degradation of PTEN and activation of the downstream AKT signalling pathway. Overall, this study revealed the oncogenic roles of miR‐92b in NSCLC by targeting PTEN/AKT, and provided novel insights for future treatments of NSCLC patients.Significance of the studyMiR‐92b was up‐regulated in human NSCLC tissues and cell lines. Our study demonstrated that miR‐92b as an oncogene in NSCLC by targeting PTEN/AKT in both in vivo and in vitro models and provided novel insights for future treatments of NSCLC patients.
Ten year follow‐up of high‐risk patients treated during the early experience with transcatheter aortic valve replacement
Authors: Sathananthan Janarthanan , Lauck Sandra , Polderman Jopie , Yu Maggie , Stephenson Anna , Sathananthan Gnalini , Moss Robert , Cheung Anson , Ye Jian , Blanke Philipp , Leipsic Jonathon , Wood David A. , Webb John G. 
Published: July 2020

AbstractBackgroundThe long‐term clinical performance of transcatheter heart valves (THV) is unknown.AimsThis study assessed the clinical outcomes, rate of structural valve deterioration (SVD) and bioprosthetic valve failure in patients after transcatheter aortic valve replacement (TAVR) to 10‐year follow‐up.MethodsConsecutive patients undergoing TAVI for native aortic valve stenosis or failed aortic surgical bioprosthesis, between 2005 and 2009 at our institution were included. A total of 235 consecutive patients.ResultsAt the time of TAVI mean age was 82.4 ± 7.9 years. All patients were judged to be high risk, with a STS score > 8 in 53.6%. THVs implanted were the Cribier‐Edwards (20.9%), Edwards SAPIEN (77.4%) or CoreValve (1.7%). Mortality at 1, 5, and 10‐year follow‐up was 23.4%, 63%, and 91.6%, respectively. Of the total cohort, 15 patients had structural valve deterioration/bioprosthetic valve failure, with a cumulative incidence at 10‐years of 6.5% (95% CI 3.3%, 9.6%). The rate of SVD/BVF at 4, 6, 8, and 10 years was 0.4%, 1.7%, 4.7%, and 6.5%, respectively. Nine patients had moderate SVD and six patients had severe SVD. Of the six patients with severe SVD, two patients had reintervention (one patient had redo TAVR, and the second had surgical aortic valve replacement). Survivors (n = 19) at 10‐year follow‐up, had a mean gradient of 14.0 ± 7.6 mmHg and aortic regurgitation ≥moderate in 5%. Quality of life measures in 10‐year survivors demonstrated ADLs 6/6 in 43.8%, and ambulation without a mobility aid of 62.5%.ConclusionUsing early generation balloon expandable THVs in a high‐risk population, there was a low rate of structural valve deterioration and valve failure at 10‐year follow‐up. This study provides insights into the long‐term performance of transcatheter heart valves and patients self‐reported derived benefits.
0.03 V Electrolysis Voltage Driven Hydrazine Assisted Hydrogen Generation on NiCo phosphide Nanowires Supported NiCoHydroxide Nanosheets
Authors: Li Mujie , Zhang Zhongyi , Xiong Hailang , Wang Linan , Zhuang Shuxian , Argyle Morris D. , Tang Yang , Yang Xiaojin , Chen Yongmei , Wan Pingyu , Fan Maohong 
Published: July 2020

AbstractIn order to decrease the electricity consumption of hydrogen generation, hydrazine‐assisted water electrolysis is intensively investigated recently. Herein, hierarchical nanostructure of ultrathin NiCo(OH)x nanosheets (NSs) that in‐situ grown on the NiCoP nanowires (NWs) was deposited on nickel foam (NF) to construct NiCo(OH)x@NiCoP/NF electrode. NiCoP NWs extend the surface area, spatial utilization of NF and enhance the electron conduction to the outmost NiCo(OH)x NSs. NiCo(OH)x NSs interlace to form regular mesoporous channels, which improve the structural stability and mass transfer rate. Moreover, NiCoP NWs enhance the adsorption of protons and the transfer of electrons, while NiCo(OH)x NSs facilitate the adsorption of OHad during reaction. As a result, NiCo(OH)x@NiCoP/NF exhibits excellent activity for both hydrazine oxidation reaction (HzOR) and hydrogen evolution reaction (HER). Based on the NiCo(OH)x@NiCoP/NF||NiCo(OH)x@NiCoP/NF couples, electrolysis of hydrazine for hydrogen generation only requires an extremely low cell voltage of 0.03 V.
Lithium Difluorophosphate as an Effective Additive for Improving the Initial Coulombic Efficiency of a Silicon Anode
Authors: Li Chao , Zhu Weicheng , Lao Banggui , Huang Xiangxuan , Yin Huibin , Yang Zhenyu , Wang Hongyu , Chen Deliang , Xu Yongjun 
Published: July 2020

AbstractSilicon anodes usually endure low initial coulombic efficiency (ICE) in applications for lithium‐ion batteries (LIBs), although the volume expansion and structural stability has been greatly improved under many efforts in the recent years. During the first charge and discharge, a large number of lithium ions can participate in the formation of a solid electrolyte interface (SEI) on anode surface, such as LiF, Li2CO3 and many Li‐based compounds, resulting in low efficiency, particularly as the nano‐sized silicon is widely applied to solve the volume effect. Herein, we find that the lithium difluorophosphate (LiPO2F2, LiDFP) additive shows some special properties, which greatly improve the reversible capacity in the first discharge. The silicon nanoparticles can deliver an ICE of 70.6 % with 2 wt% LiDFP in the electrolyte, which is increased by 17.7 % compared with the cell without LiDFP (ca. 52.9 %). By comparing the XPS and NMR results, it appears the LiDFP may reduce the number of lithium ions involved in the SEI reaction in the electrolyte during the first discharge, and thus the ICE can be improved.