bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sum-peak method with two NaI(Tl) crystals: 68(Ge+Ga) standardization
Authors: Oliveira Estela Maria , Iwahara Akira , Poledna Roberto , Da Silva Carlos José , Da Cruz Paulo Alberto Lima , Gomes Regio Dos Santos , Delgado José Ubiratan , Lopes Ricardo Tadeu 
Published: October 2019

A 68(Ge+Ga) commercial solution has been standardized in LNMRI in Brazil, by sum-peak method, in which a 3”x3” NaI(Tl) gamma-ray detector is positioned at the top of a well-type 5”x5” NaI(Tl) gamma-ray detector, resulting in a set up approximately 4p geometry. In this work the known germanium volatility was tested using three dried sources and three liquid sources in the sum-peak method measurements and the activity results showed a standard deviation of 0.41%. The activities were compared with another primary method: 4pb-g live-timed anti-coincidence counting. The two methods gave activity concentration values with differences from the certified value of +0.8 % (anticoincidence method) and -3.4% (sum-peak method).
Detection of platelet-associated immunoglobulins and complement system components in patients with aplastic anemia and hemoblastosis
Authors: Rakhmani A. F. , Mikhaylova E. A. , Galtseva I. V. , Davidova Yu. O. , Kapranov N. M. , Dubinkin I. V. , Kulikov S. M. , Gaponova T. V. , Fidarova Z. T. , Troitskaya V. V. , Parovichnikova E. N. , Savchenko V. G. 
Published: October 2019

Background. In addition to anti-HLA-I and anti-HPA-antibodies and specific cytotoxic T-lymphocytes, another cause of immune refractoriness to donor's platelet transfusions could be a platelet-associated different classes immunoglobulins PAIg (G, M, A) and C3 / C4‑components of complement system (PAC3, PAC4). These markers can be detected by flow cytofluorometry of double-stained platelets. The fixation density of immunoglobulins and components of complement systems were measured by the mean fluorescence intensity (MFI).Objective: to study additional factors that aggravate the course of refractoriness to donor's platelet transfusions in patients with aplastic anemia (AA) and hemoblastosis.Materials and methods. 77 patients (AA – 47, myelodysplastic syndrome (MDS) – 10, acute myeloid leukemia (AML) – 20) admitted to National Research Centre for Hematology during 11.09.2016–04.28.2018 were enrolled in the study. M / f ratio was 33 / 44, median age was 36 yrs. (19–71 yrs.). Plasmapheresis and cross-matching for PRP selection were used for patients with refractoriness to donor's platelet transfusion. PAIg (G, M, A) and PAC3 / C4 detection and density (MFI) were evaluated in all patients by flow cytofluorometry of doublestained platelets (CD41a-PE; IgA, M, G-FITC; C3 / C4‑FITC) and MFI measurement. Patients with AA were investigated on different stages of therapy and if refractoriness to donor's platelet transfusion is developed. Blood donors (n = 28) MFI measurement results were established as negative control.Results. It was found that MFI PAIgG/M/А and PAC3/С4 was higher in all groups of the patients (АА, MDS, AML), as compared with donors. MFI of PAIgM and PAIgA in patients were significant higher than MFI of PAIgG and PAC3 / C4. Combination of PAIgM / A, PAIgM / C3 / C4 and PAIgA / C3 / C4 were more frequent. Multiple transfusions of PRP were associated with PAIgA and PAC3 detection. Development of refractoriness to donor's platelet transfusions was accompanied by alloantibodies (HLA-I, HPA) and PAIgM, PAC4 detection. In patients of AA group during development of refractoriness to donor's platelet transfusions and multiple infection complications the high density of PAIgM and PAIgA were identified. Relapse of AA was accompanied MFI of PAC3 density increment.Conclusion. In addition to application of a certain transfusion therapy algorithm it is also necessary to detect PAIg (G, M, A) and PAC3 / C4 for prediction of severe refractoriness to donor's platelet transfusions.
Identification of common and new rare types of weak RhD antigen in patients with blood diseases and healthy person
Authors: Golovkina L. L. , Kalandarov R. S. , Pshenichnikova O. S. , Surin V. L. , Stremoukhova A. G. , Pushkina T. D. , Khasigova B. B. 
Published: October 2019

Background.Rhesus phenotype has been determined in 404 persons which have problems with blood groups identification. Genetic typing of antigen RhD variants was performed in 73 individuals. Objective of the work was to give molecular and serological characteristics of the antigen RhD weak types.Materials and methods.Method of rhesus phenotype determination in direct agglutination test on plane by using of anti-D, anti-C, anti-c, anti-Cw, anti-E and anti-e monoclonal antibodies; gel method of rhesus phenotype determination; methods of genetic typing of RhD; methods of antigen RhD determination in the classic indirect antiglobulin test and in the gel indirect antiglobulin test; method of antigen RhD determination in the saline agglutination test.Results.Serological methods identified 73 red blood samples with the weakened expression of RhD antigen. Molecular methods showed the reasons of weakness of antigen expression. Three RHD*D weak types which are common in Russians (RHD*D weak type 1–3) were identified and for the first time 3 types were found – RHD*D weak type 67, RHD(G255R) and RHD(JVS5-38del4). Serological characteristic of RhD weak types was given. It was shown that combined using of monoclonal antibodies in direct agglutination test and in gel is the most effective serological method of the antigen variants detection. Red blood cells with weak RhD antigens can be recognized by weakness or absence of agglutination with monoclonal antibodies on plane if agglutination in gel was 3+4+.Conclusion.Concrete weak RhD variants can be determined only by genetic typing. Serologically weak antigen variants can be detected by using of at least two series of monoclonal antibodies or by using of two different methods (it is preferable).
Empirical anidulafungin therapy increases the treatment efficacy in invasive candidiasis: ERA study results (EraxisR in the Russian Federation)
Authors: Klimko N. N. 
Published: October 2019

The objective of the study was to evaluate the effectiveness of empirical therapy with anidulafungin in patients with invasive candidiasis in real clinical practice in Russian healthcare institutions.Materials and methods. In a prospective multicenter (n = 23) ERA study in 2015–2017, 92 adult patients with invasive candidiasis were included. Depending on the type of anidulafungin administration, patients were divided into 2 comparable demographic groups. In the 1st group (n = 52; median age 50.5 ± 13.9 years (19–75 years); men ‒ 62 %), invasive candidiasis was microbiologically confirmed after the initiation of empirical anidulafungin therapy. In the 2nd group (n = 40; median age 47.15 ± 18.01 years (18–98 years); men — 65 %), patients received anidulafungin only after microbiological confirmation of invasive candidiasis. The groups did not statistically differ in risk factors, etiology of invasive candidiasis, the frequency of various organs damage, and also in APACHE II and SOFA score at the time of anidulafungin administration.Results and conclusion. Overall survival on the 30th day after laboratory confirmation of invasive candidiasis was statistically significantly higher in the empirical therapy group (66 % vs 58 %; p = 0.04 416). In addition, the empirical anidulafungin therapy was lead to a significant decrease in the duration of stay in intensive care units (13.5 ± 19.7 days vs 21 ± 15 days) and the total treatment time (22 ± 18 days vs 29 ± 21 days).
Pharmacoeconomic analysis of the anidulafungin (EraxisR) in adult patients with invasive candidiasis
Authors: Nedogoda S. V. , Salasyuk A. S. , Barykina I. N. , Smirnova V. O. 
Published: October 2019

Objective: to assess the economic outcomes of using anidulafungin (Eraxis®) in comparison with the recommended treatment regimens in adult patients with invasive candidiasis.Materials and methods. The economic impact assessment was carried out using “cost–effectiveness” analysis and “budget impact” analysis. “Cost–effectiveness” and “budget impact” analyses were performed in Microsoft Excel model.Results. An analysis of the effectiveness showed that the use of anidulafungin is characterized by higher survival (79.25 % vs 66.17 % for caspofungin and 60.84 % for mikafungin). The cost of anidulafungin was 377.7 thousand rubles, which is comparable with the course of caspofungin and 61 % lower than the cost of micafungin therapy. “Cost–effectiveness” analysis showed that the use of the anidulafungin in the treatment of invasive candidiasis is pharmacoeconomically effective and has a “cost–effectiveness” ratio comparable to caspofungin in terms of the effectiveness of therapy for 7 days and exceeds it in terms of the cost of preserving the patient»s life by 14.5 %. Compared to micafungin, anidulafungin is both more economical and more effective in terms of preserving the patient's life, reaching an advantage of up to 45 %. An analysis of the impact on the budget showed that with the complete replacement of the currently used treatment regimens in the target population of 11,840 patients with invasive candidiasis with anidulafungin, it is possible to reduce the burden on the budget of the healthcare system to 17 % or 1458.6 million rubles in year. The average savings in the 3‑year study horizon, taking into account the gradual switching of 25, 50 and 75 % of the target patient population, amounted to 1277.9 million rubles per year or 14.4 %. A sensitivity analysis confirmed the results.Conclusion. Based on results, it can be concluded that the use of the drug anidulafungin (Eraxis®) in patients with invasive candidiasis is economically the preferred option compared to the use of current antibiotic therapy regimens.
Study on risk factors of respiratory distress syndrome in term neonates: a retrospective case-control study
Authors: B. Sunil , Nithya E. , N. Girish 
Published: October 2019

Background: Respiratory distress syndrome (RDS) is one of the most common causes of neonatal respiratory failure and neonatal death. It is more common in preterm neonates but also been found in term and late preterm neonates. This study aims at studying the risk factors for developing RDS in term neonates. Objectives was to study the maternal and perinatal risk factors for RDS in term neonates.Methods: This is a retrospective case control study conducted in neonatal intensive care unit of tertiary care centre. A total of 100 term neonates with RDS were taken as cases and 150 normal term neonates were taken as control. Data were collected from the hospital NICU records, maternal and neonatal history was taken. The χ2 tests or Fisher’s exact text were used for one-way risk factor analysis. The effects of multiple factors on term neonatal RDS were analyzed using logistic regression analysis.Results: In our study RDS in term neonates was significantly associated with following risk factors like selective cesarean section, male sex, SGA, oligohydramnios, MSAF, severe fetal distress, birth asphyxia, PROM, and maternal-fetal infection. Among the significant risk factors severe birth asphyxia, maternal-fetal infection, PROM, MSAF were showing positive association with RDS in full-term neonates.Conclusions: Several high-risk factors such as severe birth asphyxia, maternal-fetal infection, PROM, and MSAF were closely correlated with full-term neonatal RDS. Hence these could provide a significant reference for the diagnosis and treatment of term neonatal RDS.
Comparative assessment of psoriasis area and severity index and fasting blood sugar levels in psoriatic patients with diabetes mellitus
Authors: Parmar Devendra , Salvi Dhairya 
Published: October 2019

<p class="abstract"><strong>Background:</strong> Present study was done with objectives to study the co-relation between PASI and Fasting blood sugar (FBS) levels in psoriatic patients with diabetes mellitus and to study the association between duration of topical steroid therapy and Fasting blood sugar (FBS) levels.</p><p class="abstract"><strong>Methods:</strong> Present study was conducted in the 26 patients at the Department of Dermatology at a tertiary care centre in Bhuj, Gujarat. A detailed history on the type of psoriasis, duration, associated co-morbid conditions and details of topical steroid therapy was obtained from all patients. They were examined in detail and assigned a PASI score to assess the severity of psoriasis. Patients with nail involvement were scored using N NAIL tool. Fasting blood sugar levels were noted in patients with Diabetes.<strong></strong></p><p class="abstract"><strong>Results:</strong> Age of patients ranged from 15 years to 72 years. The different types of psoriasis observed were palmoplantar psoriasis-14 (50%), and chronic plaque psoriasis - 11. The joint was involved in 7 patients while 19 did not have joint involvement. The Pearson co-relation coefficient between PASI and FBS was 0.60 and p value was 0.001. The Pearson co-relation coefficient between duration of topical steroid therapy and FBS was 0.15, and p value was 0.50.</p><p><strong>Conclusions:</strong> A statistically significant positive correlation between PASI and FBS was noted in this study. The duration of topical steroid therapy was not associated with significant changes in FBS in psoriatic patients with diabetes mellitus. </p>
A comparative study of effectiveness of autologus serum therapy with oral antihistamines versus oral methotrexate and oral antihistamines in chronic urticaria patients
Authors: Pandey Amit Kumar , Ojha Suryakant , Mohan Aayushi , Prajapati Sunil 
Published: October 2019

<p class="abstract"><strong>Background:</strong> Chronic urticaria (CU) is a worrisome problem and patients of CU suffer from the morbidity that arises from irritable itch thus they are subjected to a huge antihistamine pill burden. The symptoms are more prominent in autoreactive urticaria (AU) where auto-antibodies in blood causing recurrent flares. Therefore a need of adjunctive drug to reduce the pill burden is felt need.</p><p class="abstract"><strong>Methods:</strong> A randomized, controlled study was done. 50 patients were given AST and 50 patients were given oral pulse methotrexate (10-15 mg once weekly) along with levocetrizine in an on-demand basis in both groups. AST was given weekly for nine weeks and followed-up for a total period of 24 weeks. Urticaria total severity score (UTSS) was used to evaluate the effectiveness of treatment. Safety parameters assessed were the spontaneously reported adverse events and laboratory parameters.<strong></strong></p><p class="abstract"><strong>Results:</strong> UTSS showed significant improvement from baseline, 7th week and 8th week onwards in AST group and methotrexate group respectively. Group comparison showed significant improvement 4th week onwards.</p><p><strong>Conclusions:</strong> Autologous serum therapy as well as methotrexate both proved effective in chronic urticaria patients. On follow-up improvement is sustained for at least 3-4 months after the last injection in case of AST. </p>
Evaluation of adapalene alone versus combination therapy of adapalene with benzoyl peroxide and adapalene with clindamycin in treatment of acne vulgaris: a prospective, observational study
Authors: Parikh Nehal R. , Sood Shikha V. , Rana Devang , Chaudhary Raju , Malhotra Supriya D. 
Published: October 2019

<p class="abstract"><strong>Background:</strong> Acne vulgaris is majorly affecting adolescent population with profound negative impact on the quality of life (QOL). The objectives of the present study was to evaluate adapalene 0.1% alone vs combination therapy of adapalene 0.1% with benzoyl peroxide (BPO) 2.5% and adapalene 0.1% with clindamycin 1% in the treatment of acne vulgaris and to analyse health related QOL using the cardiff acne disability index (CADI).</p><p class="abstract"><strong>Methods:</strong> This prospective, observational study of 12 months duration involved patients who were being treated with adapalene alone and adapalene combined with either clindamycin or benzoyl peroxide in the normal course of treatment. Efficacy of treatment and QOL was assessed by the comprehensive acne severity system (CASS) and CADI respectively.<strong></strong></p><p class="abstract"><strong>Results:</strong> A total of 180 patients were enrolled (n=60 in each group). Male: female ratio was 1: 2.52. 76.7% patients were of adolescent age with the Mean age of 21.17±3.28 years. Average age of onset was 18.03±2.80 years. Most patients had moderate grade of acne (51%) followed by mild grade (46%) and almost clear (3%). Face was the common site, followed by back and chest. There was a statistically significant improvement in both number of lesions and also QOL in all the three treatment groups (p&lt;0.0001).</p><p class="abstract"><strong>Conclusions:</strong> Topical adapalene 0.1% is efficacious in the treatment of acne vulgaris both alone as well as in combination with topical benzoyl peroxide 2.5% and topical clindamycin 1%. Adapalene also has positive influence on the QOL alone as well as in combination but no superiority of one group over the other was observed with regard to efficacy as well as QOL.</p><p> </p>
Peeling skin syndrome: an investigational dilemma
Authors: Saini Shefali , Kharkar Vidya 
Published: October 2019

<p><span lang="EN-IN">Peeling skin syndrome is a rare autosomal recessive disorder of cornification that starts either at birth or later in childhood, characterized by widespread painless peeling of the skin in superficial sheets. We report the case of a 13-year-old boy who presented with asymptomatic peeling of skin since birth. Sheets of skin were peeling from the neck, trunk and extremities following mild friction or rubbing, with sparing of palms and soles. The case is being reported due to its rarity.</span></p>
Change in the Expression of BAK، FAS، BAX، TNF-A، BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B
Authors: Afzali Sara , Doosti Abbas , Heidari Mansour , Babaei Nahid , Keshavarz Parvaneh 
Published: October 2019

Introduction: Breast cancer is one of the most prevalent malignancies among women. Patients whose suffering from this condition, as a result of the use of conventional therapies often have a poor response to treatment and the relapse among them is frequent. In this study, the effects of staphylococcal enterotoxin type B on BAK، FAS، BAX، TNF-a، BCL-2 و Survivin genes expression in human breast adenocarcinoma cells (MCF-7) was examined. Staphylococcal enterotoxin B is a powerful member of the Staphylococcus aureus toxins family, which is known as an anticancer agent with potential for killing cancer cells.
Methods: The experimental study was carried out at the Biotechnology Research Center of Islamic Azad University, Shahrekord Branch. By using Lipofectamine 2000 reagent, MCF-7 cells transfected with the pcDNA3.1(+)-seb (recombinant) and pcDNA3.1(+) (non-recombinant) plasmids and were selected by culturing in a selective medium of RPMI- 1640 containing 600 μg / mL antibiotic G418. Then, the expression of BAK, FAS, BAX, TNF-a, BCL-2, and Survivin genes in transfected cells were analyzed by real time PCR. Student's t-test, SPSS Inc., Chicago, IL; Version 16 and also Excel program for statistical analysis were used.
Results: The results of this study indicated that staphylococcal enterotoxin type B (SEB) remarkably changes the expression of apoptotic related genes in MCF-7 cell line. It was observed a significant increase in the expression of BAK, FAS, BAX, and TNF-a genes, the expression of BCL-2 and Survivin genes significantly decreased compared to the control group (P=0/032).
Conclusion: Staphylococcal enterotoxin type B has an inhibitory effect on the growth, proliferation and invasion of breast adenocarcinoma cells through altering the expression of the genes involved in the apoptosis process. Therefore, it seems that there is a good research field for the use of this toxin in the control and treatment of human breast adenocarcinoma.
Retrospective evaluation of indications and birth results of cesarean section due to ophthalmologic diseases
Authors: COŞKUN Bora , ÖZATEŞ Serdar , COŞKUN Buğra , KINCI Mehmet Ferdi , ŞİMŞİR Coşkun 
Published: October 2019

Objectives: To investigate the outcomes of cesarean section due to ophthalmologic indications. Methods: This retrospective clinical study included 40,190 patients underwent cesarean section due to ophthalmological indications between January 2013 – August 2015 in MLP Care Group Hospitals (It includes 32 hospitals). Demographic data, ophthalmologic history and indications and surgical outcomes of caesarean section was assessed.Results: Of the 53 patients with caesarean delivery due to ophthalmologic indications, 32 (60.4%) had high degree myopia, 7 (13.2%) had degenerative myopia, 8 (15.1%) had history of ocular surgery, 2 (3.8%) had history of vitreoretinal surgery due to retinal detachment, 2 (3.8%) had glaucoma with uncontrolled intraocular pressure, 1 (1.9%) had retinitis pigmentosa and 1 (1.9%) had optic neuritis.Conclusions: Ratio of ophthalmic indications is small within all cesarean indications and further longitudinal studies are needed to prevent from unnecessary cesarean section.
Los eolismos en la lengua homérica
Authors: Crespo Emilio 
Published: October 2019

Uno de los hallazgos más importantes de la filología clásica durante el siglo XX es que los poemas homéricos están al final de una tradición oral, en el curso de la cual poetas iletrados improvisaban canciones heroicas sin ayuda de la escritura y las difundían de manera oral. Sobre esta base, este artículo intenta explicar por qué los poemas homéricos presentan rasgos dialectales eólicos, siendo su forma lingüística mayoritariamente jónica. En concreto, se ofrecen argumentos en favor de que los eolismos dialectales de la Ilíada y de la Odisea entraron en la tradición oral como préstamos tomados de dialectos eólicos o de canciones orales épicas que usaban básicamente una forma dialectal eólica. Si los argumentos expuestos son ciertos, el resultado es que los eolismos dialectales, lejos de apuntar a la existencia de una fase eólica anterior a una jónica, prueban que la tradición oral fue única y sin solución de continuidad desde época de comunidad jónico-ática en adelante.
Para los agricultores inexpertos: nuevos conocimientos sobre las Geórgicas de Virgilio
Authors: Erren Manfred 
Published: October 2019

El año 29 a. C., en vísperas de que Octaviano celebre su triunfo, Virgilio le presenta su programa de gobierno, en forma de poema. Los soldados se establecerán como nuevos agricultores y, con los modestos campesinos, llevarán la vida feliz y pacífica de los sabios, en Italia, la tierra más hermosa del mundo, ahora centro del imperio romano. Sobre una base de poesía didáctica antigua, de empirismo peripatético y de filosofía epicúrea de la naturaleza, Virgilio coloca en ese programa un esbozo de agricultura y de astronomía, carente de aspectos comerciales, científicamente moderno y poéticamente elaborado. El epilio que está al final del poema era, originalmente, un texto para una pantomima que se mofa de la creencia en la inmortalidad de las almas, pero Virgilio lo añade a las Geórgicas como pieza didáctica sobre la muerte y el renacimiento.
La libertad asediada: Pompeyo frente a César en la Farsalia
Authors: Gaos Schmidt Amparo 
Published: October 2019

Los personajes secundarios masculinos y, muy especialmente, los femeninos, constituyen uno de los medios por los cuales Lucano confiere fundamento y vigor a las imágenes que quiere ofrecer de César como ejemplo de la lujuria, el dispendio, la deshonestidad y todas las demás oscuras fuerzas que amenazaban devastar a la Urbe Eterna, y de Pompeyo como campeón de la libertad y espejo de virtudes romanas tradicionales, las cuales para el poeta, al igual que para Ennio, constituían el sustento mismo de la grandeza de Roma.