bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioinformatics Network Analyses of Growth Differentiation Factor 11 Anti-aging Study
Authors: Zhang Feng , Yang Xia , Bao Zhijun 
Published: December 2020

Abstract
Growth differentiation factor 11 (GDF11) has been implicated in rejuvenative functions in age-related diseases. The molecular mechanisms connecting GDF11 with these anti-aging phenomena (reverse age-related cardiac hypertrophy, vascular and neurogenic rejuvenation, et al.) are still unclear. In this study, we aim to uncover the molecular functions of GDF11 using bioinformatics and network-driven analyses at human gene and transcription levels using gene co-expression network analysis and transcription factor network analysis. Our data suggests that GDF11 is involved in variety of functions such as apoptosis, DNA repair and telomere maintenance and interaction with key transcription factors such as MYC proto-oncogene (MYC), specificity protein 1 (SP1) and ETS proto oncogene 2 (ETS2). Our findings shed lights on the functions of GDF11 and provide insights into the role of GDF11 in aging.
Are Nurses at Swedish Departments of Infectious Diseases Prepared to Care for Patients With African Viral Haemorrhagic Fever? - A Survey Study
Authors: Blixt Sofia Jakobsson , Gamalielsson Susann , Manninen Katri 
Published: December 2020

Abstract
Background: The African viral haemorrhagic fevers have in recent years been causing large outbreaks with high mortality rates and elevated risks of global spread. These outbreaks puts the Departments of Infectious diseases, both national and international, on high demand when caring for this patient group, in a patient- and staff-safe manner. The aim of the study was to describe nurses perceived ability and knowledge about caring for patients with suspected or verified African viral haemorrhagic fever at Departments of Infectious diseases in Sweden. Method: A web survey was conducted to collect data. The results are presented through a descriptive design. Participants were registered nurses working in infectious diseases clinics; 216 survey results were registered. Results: Registered Nurses in Swedish Departments of Infectious diseases clinics witnessed about having limited knowledge about the African haemorrhagic fevers. They were also experiencing limited or very limited knowledge about some practical procedures, like drawing blood samples to confirm the infection. The majority of the participants had not been given theoretical education, nor had been given the opportunity to sufficiently practice using personal protective equipment at their place of work. The nurses witnessed about fear for their own safety while caring for this group of patients. Conclusion: The participants perceived about fear, both limited theoretical and practical knowledge and training about caring for patients with African haemorrhagic fever, even though they had worked with infectious diseases for several years. There is a need for implementation of measures to ensure the healthcare professionals' safety and to prevent them from being infected with potentially lethal infections. It also poses a risk for the patient in the absence of specific nursing care, which can lead to an increased critical disease state.
Construction of a Nomogram Model to Predict The Risk of Hypothyroidism in Patients With Thyroid Nodule Caused by Environmental Iodine and Fluorine
Authors: Yan Ruixia , Yang Dongliang , Li Yanguo , Zhou Yuan , Pang Yaxian , Li Shuzhen , Yang Guiran , Xu Ruoqi , Zhang Tong , Yang Fengyan , Zhang Chunhe , Hu Caihong , Zhang Juan , Zhang Qiuyu , Liu Mingqing 
Published: December 2020

Abstract
Background: Patients with thyroid nodules may be complicated by hypothyroidism. However, effective means to predict thyroid-stimulating hormone (TSH) elevation in patients with thyroid nodules are currently lacking. Therefore, the research team recruited some patients with thyroid nodules to explore the relationship between TSH, environmental iodine, and fluoride and the development of thyroid nodules, and to draw a Nomogram in order to provide guidance for predicting hypothyroidism in patients with thyroid nodules.Methods: The subjects were from 313 patients with thyroid nodules in different areas of iodine and fluoride, with 71 men and 242 women. The content of TSH in serum of thyroid nodule patients was determined by electrochemical luminescence method. The iodine content in drinking water and urine was determined by arsenic-cerium catalytic spectrophotometry. Fluoride in drinking water and urine was determined by fluoride ion selective electrode method. Univariate analysis, Lasso regression analysis and multiple factor Logistic regression analysis were used to screen the variables included in the Nomogram model. And the corresponding Nomogram model was constructed. ROC curve was drawn and repeated Bootstrap self-sampling method was used to test model differentiation.Results: There were 53 patients with elevated serum TSH and 256 patients with normal thyroid nodule. Multivariate logistic regression analysis shows that urine iodine（Odds Ratio[OR]=1.001, 95% confidence interval [95%CI]:1.001～1.002, P=0.01） and gender (OR=3.328, 95% CI:1.256～8.819, P=0.079) are independent risk factors raised by serum TSH. And drinking water fluoride（OR=1.990, 95% CI:0.924～4.289, P=0.079）is a critical factor. Based on these risk factors, the predictive model and nomogram of serum TSH elevation in patients with thyroid nodule were constructed. The area under the ROC curve was 0.678 (P<0.001), confirming Nomogram's predictive potential. The calibration diagram of the Nomogram prediction model shows that the predicted values are in good agreement with the observed values.Conclusion: Gender, urine iodine and drinking water fluoride of thyroid nodules are independent influencing factors for the rise of serum TSH. The Nomogram can become a predictive accuracy and differentiation.
Development of Alpha-emitting radioembolization for Hepatocellular Carcinoma: Longitudinal Monitoring of Actinium-225’s Daughters Through SPECT Imaging
Authors: Du Yong , Cortez Angel , Zarisfi Mohammadreza , Josefsson Anders , Krimins Rebecca , Liapi Eleni , Nedrow Jessie R 
Published: December 2020

Abstract
Hepatocellular carcinoma is the most common primary liver cancer and the fifth most frequently diagnosed cancer worldwide. Most patients with advanced disease are offered non-surgical palliative treatment options. This work explores the first α-emitting radioembolization for the treatment and monitoring of hepatic tumors. Furthermore, this works demonstrates the first in vivo simultaneous multiple-radionuclide SPECT images of the complex decay chain of an [225Ac]Ac-labeled agent using a clinical SPECT system to monitor the temporal distribution. Methods: A DOTA chelator was modified with a lipophilic moiety and radiolabeled with Actinium-225. The resulting agent, [225Ac]Ac-DOTA-TDA, was emulsified in Lipiodol® and evaluated in vivo in mouse model and the VX2 rabbit technical model of liver cancer. SPECT imaging was performed to monitor distribution of the TAT agent and the free daughters.Results: [225Ac]Ac-DOTA-TDA was shown to retain within the HEP2G tumors and VX2 tumor, with minimal uptake within normal tissue. In the mouse model, significant improvements in overall survival were observed. SPECT imaging was able to distinguish between the Actinium-225 agent (Francium-221) and the loss of the longer lived daughter, Bismuth-213. Conclusion: A TAT agent emulsified in Lipiodol® is capable of targeting liver tumors with minimal accumulation in normal tissue, providing a potential therapeutic agent for the treatment of HCC as well as a variety of hepatic tumors. In addition, SPECT imaging presented here provides a foundation for imaging methodology and protocols that can be rapidly translated into the clinic to monitor Actinium-225-labeled agents.
Predicting The Risk and Timing of Bipolar Disorder In Offspring of Bipolar Parents: Exploring The Utility of a Neural Network Approach
Authors: Cooper Alysha , Horrocks Julie , Goodday Sarah Margaret , Keown-Stoneman Charles , Duffy Anne 
Published: December 2020

Abstract
BackgroundBipolar disorder onset peaks over early adulthood and confirmed family history is a robust risk factor. However, penetrance within families varies and most children of bipolar parents will not develop the illness. Individualized risk prediction would be helpful for identifying those young people most at risk and to inform targeted intervention. Using prospectively collected data from the Canadian Flourish High-Risk Offspring cohort study available in routine practice, we explored the use of a neural network, known as the Partial Logistic Artificial Neural Network (PLANN) to predict the time to diagnosis of bipolar spectrum disorders Results Overall, for predictive performance, PLANN outperformed the more traditional logistic model for one year, three year and five-year predictions. PLANN was better able to discriminate or rank individuals based on their risk of developing bipolar disorder, better able to predict the probability of developing bipolar disorder and had higher accuracy than the logistic model. ConclusionsThis evaluation of PLANN is a useful step in the investigation of using neural networks as tools in the prediction of diagnosis of mental health for at-risk individuals and demonstrated the potential that neural networks have in this field. Future research is needed to replicate these findings in a separate high-risk sample.
Educational Knowledge, Attitude and LGBT Inclusive Training in an Academic Setting: A Mixed Methods Case Study of Faculty and Healthcare Students in Nigeria.
Authors: Sekoni Adekemi , Jolly Kate , Gale Nicola 
Published: December 2020

Abstract
Background: The highest quality of appropriate care is required to achieve the UN’s Sustainable Development Goals and improve the health of the LGBT population in the global south. This study assessed the training, knowledge, beliefs, attitudes and practice of Nigerian healthcare students and faculty living, working and learning within the constraints of religious, legal and cultural barriers for LGBT people.Methods: This study was carried out among all the tutors in the faculty of clinical sciences, year five medical and nursing students in the College of Medicine, University of Lagos. The sequential mixed methods study design was guided by the socio-ecological model of health. Quantitative data were collected with an adapted questionnaire. The results were used to generate prompts for the qualitative component consisting of In-depth interviews with 21 of the respondents. Transcripts were analysed using thematic analysis. The quantitative and qualitative data were subsequently integrated and, using the socio-ecological model, synthesized.Results: The response rate was 88.2% (medical students), 66.2% (nursing students) and 60.7% (tutors). Themes uncovered were: knowledge of LGBT terminologies; teaching and learning about LGBT health topics; ethics, professionalism and personal beliefs. A fifth of tutors reported teaching related topics or using LGBT examples in class. Students were more knowledgeable of LGBT terminologies than tutors. Facilitators and barriers to LGBT inclusion in healthcare education settings were identified within the following socio-ecological domains: globalization, policy, community, institution, intrapersonal and individual. Conclusion: Ongoing advocacy with policy makers and educational leaders on the right to health for all citizens was recommended. A multilevel intervention by non LGBT and LGBT individuals is required for inclusion of LGBT health in educational curriculum of healthcare students and professionals.
Association between life style factors and sub-fertility among women, attend two specials hospital for women in Colombo: A case control study
Authors: Piyasenage Munasinghe P.M , - Balachandran Kumarendran , - Chandani Denawaka , - Kosala Lakmal , - Kumari Illukpitiya 
Published: December 2020

Abstract
Background: Sub-fertility has become a dominant health issue among married couples, expecting a baby with impacting social, psychological, and financial consequences especially in developing countries. As well as life style factors, which cause to immense effect on reproductive health has been changing dramatically at present. This study accordingly determines the association between life style factors and sub-fertility of women. Method: 125 of case and 320 of control were enrolled. A pre tested structured questionnaire was used to collect the data through face to face interview. Chi-square, Man Whitney and independent sample T tests were performed (SPSS-22 version). Furthermore Odds Ratio (OR) was done for passive smoking and entertains with music. Result: The mean age at marriage was 25.1±3.1 and 26.5±3 for fertile sub-fertile women respectively. Vigorous and moderate physical activities were significantly high among sub-fertile than fertile (P<0.05 C.I 95%), but total sitting time was not significantly different between two groups (P=0.16 CI 95%). Time of sedentary hobbies were high among fertile than sub-fertile (P=0.00 CI 95%). OR for passive smoking was 1.32 CI 95% and it was 0.6 CI 95% for entertainment with music. Invoking blessing of god is the most common religious practices among both groups. Conclusion: This study demonstrated that the moderate physical activities and sedentary hobbies are not associated with fertility and second hand smoking has a negative effect while entertainment with music has a positive effect to the fertility.
The Association of Baseline N-terminal Pro-B-type Natriuretic Peptide With Short and Long-term Prognosis Following Percutaneous Coronary Intervention in Non-ST Segment Elevation Acute Coronary Syndrome With Multivessel Coronary Artery Disease: a Retrospective Cohort Study
Authors: He Wen-fei , Jiang Lei , Chen Yi-yue , Liu Yuan-hui , Chen Peng-yuan , Duan Chong-yang , Zeng Li-huan , Fan Hua-lin , Wei Xue-biao , Guo Wei , Chen Wei , Li Jun , Li Wen-sheng , Guo Zhi-qiang , Liu Zhi-kai , Tan Ning , Chen Ji-yan , He Pengcheng 
Published: December 2020

Abstract
Background: Although several studies have shown that N-terminal pro-B-type natriuretic peptide (NT-proBNP) is strongly correlated with coronary artery lesion complexity as well as prognosis in non-ST segment elevation acute coronary syndrome (NSTE-ACS) patients, the prognostic value of NT-proBNP in patients with NSTE-ACS and multivessel coronary artery disease undergoing PCI remains unclear. This study aimed to reveal the relationship between NT-proBNP levels and prognosis among NSTE-ACS patients with multivessel coronary artery disease undergoing successfully percutaneous coronary intervention.Methods: We consecutively enrolled 1022 patients from January 2010 to December 2014. Patients with a diagnosis of NSTE-ACS with multivessel coronary artery disease and NT-proBNP levels were included. The primary outcome was in-hospital all-cause death. The 3-year follow-up all-cause death was also ascertained.Results: A total of 12 (1.2%) deaths occurred during hospitalization. The 4th quartile group of NT-proBNP (>1287 pg/ml) had the highest rate of in-hospital all-cause death (4.3%) (P<0.001). Logistic analyses revealed that increasing NT-proBNP was robustly associated with a higher risk of in-hospital all-cause death (adjusted OR: 2.86, 95% CI=1.16-7.03, P=0.022). NT-proBNP had a good ability to predict in-hospital all-cause death (AUC=0.888, 95% CI=0.834-0.941, P<0.001; cutoff: 1568pg/ml). The cumulative event analyses exhibited a statistically significant relationship between a higher level of NT-proBNP and a higher rate of the long-term all-cause death compared with a lower level of NT-proBNP (P< 0.0001).Conclusions: Increasing NT-proBNP is significant associated with a high risk of in-hospital and long-term all-cause death in NSTE-ACS patients with multivessel coronary artery disease who received percutaneous coronary intervention. NT-proBNP > 1568pg/ml was associated with all-cause, in-hospital death.
Proteomic And Metabolomic Analyses Reveal The Full Spectrum of Inflammatory and Lipid Metabolic Abnormalities In Dyslipidemia
Authors: DU Hui , RAO Yifei , LIU Ronghua , DENG Kesui , GUAN Yongmei , LUO Dewei , MAO Qiping , YU Jianwei , BO Tao , FAN Ziquan , ouyang hui , FENG Yulin , ZHU Weifeng 
Published: December 2020

Abstract
Background: Dyslipidemia is a common, chronic metabolic disease associated with cardiovascular complications. Due to the multiplicity of etiological factors, the pathogenesis of dyslipidemia is still unclear.Methods: In this study, we combined proteomics and metabolomics methods to analyze the plasma of patients with dyslipidemia and healthy subjects. ITRAQ markers, combined with LC-MS/MS proteomics technology and the UHPLC/ Orbitfast-X Tribrid system, were used to establish the metabolite profile in clinical dyslipidemia.Results: A total of 137 differentially expressed proteins were identified, mainly related to biological processes such as protein activation cascades, adaptive immune responses, complement activation, acute inflammatory responses and regulation of acute inflammatory responses. These proteins are involved in the regulation of important metabolic pathways, such as immunity and inflammation, coagulation and hemostasis, lipid metabolism, and oxidation and antioxidant defenses. Analysis of clinical metabolites showed there were 69 different metabolites in plasma, mainly related to glycerolipid, sphingolipid, porphyrin, alpha-linolenic acid, linoleic acid and arachidonic acid metabolism, suggesting that regulation of inflammation and lipid metabolism may be disturbed in patients with dyslipidemia. Among these, significant changes were observed in indole-3-propionic acid (IPA), which is considered a potential biomarker of dyslipidemia. Conclusions: Combined analysis of proteins and metabolites showed that arachidonic acid, linoleic acid and lipid metabolic pathways were closely related to dyslipidemia. IPA may be a potential biomarker.The information provided in this study may provide new insights into the pathogenesis of dyslipidemia and related diseases, as well as potential intervention targets.
Serum Anti-AP3D1 Antibodies Are Broad-Spectrum Biomarkers for Atherosclerosis, Acute Ischemic Stroke, Cardiovascular Disease, Diabetes Mellitus, Chronic Kidney Disease, and Digestive Organ Cancer
Authors: Li Shu-Yang , Yoshida Yoichi , Kobayashi Eiichi , Kubota Masaaki , Matsutani Tomoo , Mine Seiichiro , Machida Toshio , Wada Takeshi , Aotsuka Akiyo , Maezawa Yoshiro , Takemoto Minoru , Yokote Koutaro , Kobayashi Yoshio , Kitamura Kenichiro , Takizawa Hirotaka , Kashiwado Koichi , Sata Mizuki , Yamagishi Kazumasa , Iso Hiroyasu , Sawada Norie , Tsugane Shoichiro , Kobayashi Sohei , Matsushita Kazuyuki , Nomura Fumio , Matsubara Hisahiro , Sumazaki Makoto , Ito Masaaki , Yajima Satoshi , Shimada Hideaki , Iwase Katsuro , Ashino Hiromi , Zhang Xiao-Meng , Wang Hao , Goto Kenichiro , Tomiyoshi Go , Shinmen Natsuko , Nakamura Rika , Kuroda Hideyuki , Iwadate Yasuo , Hiwasa Takaki 
Published: December 2020

Abstract
Background: Atherosclerosis has been considered as the main cause of morbidity, mortality, premature incapacity, and disability worldwide. For early and sensitive diagnosis, development of novel biomarkers is expected and of significant practical importance.Methods: The first screening for antigen markers was conducted using the serological identification of antigens by recombinant cDNA expression cloning (SEREX). Serum antibody levels were examined using the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) using a recombinant protein as an antigen.Results: SEREX screening has identified adaptor-related protein complex 3 subunit delta 1 (AP3D1) as an antigen recognized by serum IgG antibodies of patients with atherosclerosis. As per the results of AlphaLISA, it was determined that the serum antibody levels against AP3D1 were higher in patients with acute ischemic stroke (AIS), transient ischemic attack (TIA), diabetes mellitus (DM), cardiovascular disease (CVD), chronic kidney disease (CKD), esophageal squamous cell carcinoma (ESCC), and colorectal carcinoma than those in the healthy donors. The area under the curve values of DM, nephrosclerosis type of CKD, and ESCC calculated using receiver operating characteristic curve analysis were noted to be higher than that of other diseases. Correlation analysis showed that the anti-AP3D1 antibody levels were highly associated with maximum intima-media thickness, which indicates that this marker reflected the development of atherosclerosis. The Japan Public Health Center-based Prospective Study results have verified that the serum antibody levels against AP3D1 showed significantly higher odds ratios with the risk of AIS for persons with the third quartiles and the highest quartiles versus the lowest quartile, indicating that this antibody marker is deemed useful as risk factors for AIS.Conclusions: Serum anti-AP3D1 antibodies, which are broad-spectrum biomarkers of atherosclerotic diseases and digestive organ cancers, could be useful in predicting the onset of AIS.
Cancer-Associated Fibroblasts Release Exosomal CBFB&nbsp;That Dictate an Aggressive Bone Metastasis Phenotype in Breast Cancer
Authors: Su Chih-Ming , Lai Yu-Hsin , Bamodu Oluwaseun Adebayo , Kuo Kuang-Tai , Fong Iat-Hang , Yeh Chi-Tai , Lee Wei-Hwa , Huang Wen-Chien 
Published: December 2020

Abstract
The authors have requested that this preprint be removed from Research Square.
Burnout and Peritraumatic Distress of Healthcare Workers in the COVID-19 Pandemic
Authors: Jang Yeonhhon , You Myoungsoon , Lee Heeyoung , lee Minjung , Lee Yeji , Han Jin-ok , Oh Jeonghyun 
Published: December 2020

Abstract
Background
To evaluate the current status of emotional exhaustion and peritraumatic distress of healthcare workers (HCWs) in the COVID-19 pandemic, and identify factors associated with their mental health status.
Methods
An online survey involving 1,068 HCWs that included nurses, physicians, and public health officers was conducted. Descriptive statistics and multivariate regression analyses were performed on the collected data.
Results
Although no significant difference in peritraumatic distress was observed among the surveyed HCWs, the workers’ experience of emotional exhaustion varied according to work characteristics. Respondents who were female, older, living with a spouse, and/or full-time workers reported higher levels of emotional exhaustion. Public health officers and other medical personnel who did not have direct contact with confirmed patients and full-time workers had a higher level of peritraumatic distress. Forced involvement in work related to COVID-19, worry about stigma, worry about becoming infected, and perceived sufficiency of organizational support negatively predict emotional exhaustion and peritraumatic distress.
Conclusions
Job-related and emotional stress of HCWs should not be neglected. Evidence-based interventions and supports are required to protect HCWs from mental illness and to promote mental health of those involved in the response to the COVID-19 pandemic.