bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRISPR/Cas9 mediated GFP‐human dentin matrix protein 1 (DMP1) promoter knock‐in at the ROSA26 locus in mesenchymal stem cell for monitoring osteoblast differentiation
Authors: Shahabipour Fahimeh , Oskuee Reza Kazemi , Shokrgozar Mohammad Ali , Naderi‐Meshkin Hojjat , Goshayeshi Lena , Bonakdar Shahin 
Published: October 2020

AbstractBackgroundDentin matrix protein 1 (DMP1) is highly expressed in mineralized tooth and bone, playing a critical role in mineralization and phosphate metabolism. One important role for the expression of DMP1 in the nucleus of preosteoblasts is the up‐regulation of osteoblast‐specific genes such as osteocalcin and alkaline phosphatase1. The present study aimed to investigate the potential application of human DMP1 promoter as an indicator marker of osteoblastic differentiation.MethodsIn the present study, we developed DMP1 promoter‐DsRed‐GFP knock‐in mesenchymal stem cell (MSCs) via the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system that enabled automatic detection of osteoblast differentiation. With the application of a homology‐directed knock‐in strategy, a 2‐kb fragment of DMP1 promoter, which was inserted upstream of the GFP and DsRed reporter cassette, was integrated into the human ROSA locus to generate double fluorescent cells. We further differentiated MSCs under osteogenic media to monitor the fate of MSCs. First, cells were transfected using CRISPR/Cas9 plasmids, which culminated in MSCs with a green fluorescence intensity, then GFP‐positive cells were selected using puromycin. Second, the GFP‐positive MSCs were differentiated toward osteoblasts, which demonstrated an increased red fluorescence intensity. The osteoblast differentiation of MSCs was also verified by performing alkaline phosphatase and Alizarin Red assays.ResultsWe have exploited the DMP1 promoter as a predictive marker of MSC differentiation toward osteoblasts. Using the CRISPR/Cas9 technology, we have identified a distinctive change in the fluorescence intensities of GFP knock‐in (green) and osteoblast differentiated MSCs 2.ConclusionsThe data show that DMP1‐DsRed‐GFP knock‐in MSCs through CRISPR/Cas9 technology provide a valuable indicator for osteoblast differentiation. Moreover, The DMP1 promoter might be used as a predictive marker of MSCs differentiated toward osteoblasts.
Mannan activates tissue native and IgE-sensitized mast cells to proinflammatory response and chemotaxis in TLR4-dependent manner
Authors: Żelechowska Paulina , Brzezińska-Błaszczyk Ewa , Różalska Sylwia , Agier Justyna , Kozłowska Elżbieta 
Published: October 2020

Abstract
Mast cells take part in host defense against microorganisms as they are numerous at the portal of infection, exert several essential mechanisms of pathogen destruction, and they express pattern recognition receptors. Accumulating evidence indicates that these cells are involved in the control and clearance of bacterial, viral, or parasitic infections, but much less is known about their contribution in defense against fungi. The study was aimed to establish whether mannan, which comprises an outermost layer and major structural constituent of the fungal cell wall, may directly stimulate tissue mast cells to the antifungal response. Our findings indicate that mannan activates mast cells isolated from the rat peritoneal cavity to initiate the proinflammatory response. We found that mannan stimulates mast cells to release histamine and to generate cysteinyl leukotrienes, cytokines (IFN-γ, GM-CSF, TNF), and chemokines (CCL2, CCL3). It also increased the mRNA expression of various cytokines/chemokines. We also documented that mannan strongly activates mast cells to generate reactive oxygen species and serves as a potent chemoattractant for these cells. Furthermore, we established that mannan-induced activity of mast cells is mediated via TLR4 with the involvement of the spleen tyrosine kinase molecule. Taking together, our results clearly support the idea that mast cells act as sentinel cells and crucially determine the course of the immune response during fungal infection. Additionally, presented data on IgE-coated mast cells suggest that exposure to fungal mannan could influence the severity of IgE-dependent diseases, including allergic ones.
Decreasing wheat yield stability on the North China Plain: Relative contributions from climate change in mean and variability
Authors: Liu Weihang , Ye Tao , Shi Peijun 
Published: October 2020

AbstractThere has been increasing interest in understanding climate change impacts on crop yield stability, including interannual yield variability and lower yield extremes, in addition to mean yield. In this study, we evaluated these impacts on wheat yield and investigated the contribution of changes in climate mean and variability, and their interaction, on the North China Plain (NCP). Wheat yield simulation experiments with control groups were conducted using the Crop Environment Resource Synthesis (CERES) model, with multiple general circulation model ensembles under two representative concentration pathways (RCPs), namely 4.5 and 8.5. Climate change was projected to reduce mean yield by 15 and 17%, increase yield interannual variability by 5 and 11%, and reduce 10‐year return period lower yield extremes by 31 and 34% under RCPs 4.5 and 8.5, respectively. When analysed, changes in climate mean proved the main cause for changes in mean yield (62–71%), followed by the interactive changes in climate mean and variability (26–33%). As for the impact on yield variability, the interaction of the changes in climate mean and variability proved the main cause (48–54%), followed by changes in climate mean (33–41%). Surprisingly, climate change in variability contributed the least in both cases. Our results pertaining to the decrease in both availability and stability of wheat yield on the NCP presents a greater challenge for building a resilient food system for local areas than before. They also highlighted the importance of separating the impacts of changes in climate mean and variability on crop yield stability in a holistic framework, with particular attention paid to the tangible and interactive effects.
Systemic osteoprotegerin does not improve peri‐implant bone volume or osseointegration in rabbits
Authors: Choi Joseph H. , Wang Zhiwei , Ross Frederick P. , van der Meulen Marjolein C. H. , Bostrom Mathias P. G. 
Published: October 2020

AbstractAnti‐RANKL (receptor activator of nuclear factor kappa‐B ligand) agents function by blocking the differentiation of osteoclasts, thereby proving useful in the clinical management of postmenopausal osteoporosis. The effects of such agents on osseointegration is less well understood. The purpose of the current study was to investigate whether osteoprotegerin (OPG), an osteoclast inhibitor, enhances the known anabolic effects of mechanical loading (VEH) and intermittent PTH (iPTH) using a well‐established rabbit model of osseointegration. In the first set of experiments, OPG was administered either alone or combined with iPTH to study its effects on measured bone mass. The second set of experiments was conducted using a higher dosage of OPG (10 mg/kg) to explore its early impact at the cellular and molecular levels. All subjects had mechanical load applied to the implant on one extremity, and no load applied on the contralateral side. In the first set of experiments, OPG alone decreased peri‐implant bone mass compared to the mechanical loading group, whereas OPG + iPTH increased peri‐implant bone mass compared to the OPG group. In the second set of experiments, high‐dose OPG significantly decreased osteoclast number (−74.3%) at 1 week. However, this effect was not sustained as osteoclast number returned to baseline by 2 weeks. These results suggest that systemic administration of OPG does not enhance osseointegration, but rather has a detrimental effect.
Assessment of the impact of pharmacy learners on admission medication reconciliation in Toronto, Canada
Authors: Chow Matthew , Lui Philip , Cameron Karen , Romanko Anatoliy , Hamandi Bassem , Gorman Sean , Harrison Jennifer , Murphy Laura , Cameron Andrea , Toombs Kent , Meade Andrea , Wong Gary , Dara Celina , Woods Amita , Lutfy Francesca , Raman‐Wilms Lalitha , Slavik Richard , Spina Sean , Rubin Bonita , Fernandes Olavo 
Published: October 2020

AbstractIntroductionCurrently there is a lack of published data examining the clinical impact of pharmacy learners on patient care outcomes in acute care. A collaborative of hospital pharmacists in Canada established consensus on eight clinical pharmacy key performance indicators (cpKPIs) representing essential patient processes of care. Of the eight cpKPIs, admission medication reconciliation has been established as a cornerstone patient care process. The implementation of cpKPI measurement creates an opportunity to quantify pharmacy learner contribution to patient care.AimTo determine if the presence of pharmacy learners partnering with pharmacists is associated with an increased number of patients receiving admission medication reconciliation (AMR).MethodsIn this prospective observational study, pharmacists and learners (on 5‐week rotations) tracked patients receiving AMR in the electronic health record from 25 January to 17 July 2016. The number of patients receiving AMR were compared during timeframes when a learner was present (intervention) to when a learner was not present (control).ResultsIn the main analysis of 30 learner‐pharmacist pairs with 4684 patients, 1136 patients received AMR in the intervention group versus 887 patients in the control group (adjusted for 5 weeks). The number of patients receiving AMR in the presence of a pharmacy learner partnered with a pharmacist (median = 43, IQR = 23–59) was significantly increased compared to the presence of a pharmacist alone (median = 36, IQR = 17–53, p < 0.001). Learners partnered with pharmacists to perform AMR for 41% of the patients.ConclusionOverall, pharmacy learners partnering with pharmacists increased the number of patients receiving AMR.
Water‐Borne Isocyanate‐Free Polyurethane Hydrogels with Adaptable Functionality and Behavior
Authors: Bourguignon Maxime , Thomassin Jean‐Michel , Grignard Bruno , Vertruyen Bénédicte , Detrembleur Christophe 
Published: October 2020

AbstractPolyurethane hydrogels are attractive materials finding multiple applications in various sectors of prime importance; however, they are still prepared by the toxic isocyanate chemistry. Herein the facile and direct preparation in water at room temperature of a large palette of anionic, cationic, or neutral polyurethane hydrogels by a non‐isocyanate route from readily available diamines and new hydrosoluble polymers bearing cyclic carbonates is reported. The latter are synthesized by free radical polymerization of glycerin carbonated methacrylate with water‐soluble comonomers. The hydrogel formation is studied at different pH and its influence on the gel time and storage modulus is investigated. Reinforced hydrogels are also constructed by adding CaCl2 to the formulation that in‐situ generates CaCO3 particles. Thermoresponsive hydrogels are also prepared from new thermoresponsive cyclic carbonate bearing polymers. This work demonstrates that a multitude of non‐isocyanate polyurethane hydrogels are easily accessible under mild conditions without any catalyst, opening new perspectives in the field.
Temperature Variation Enables the Design of Biobased Block Copolymers via One‐Step Anionic Copolymerization
Authors: Bareuther Jennifer , Plank Martina , Kuttich Björn , Kraus Tobias , Frey Holger , Gallei Markus 
Published: October 2020

AbstractA one‐pot approach for the preparation of diblock copolymers consisting of polystyrene and polymyrcene blocks is described via a temperature‐induced block copolymer (BCP) formation strategy. A monomer mixture of styrene and myrcene is employed. The unreactive nature of myrcene in a polar solvent (tetrahydrofuran) at −78 °C enables the sole formation of active polystyrene macroinitiators, while an increase of the temperature (−38 °C to room temperature) leads to poly(styrene‐block‐myrcene) formation due to polymerization of myrcene. Well‐defined BCPs featuring molar masses in the range of 44–117.2 kg mol−1 with dispersities, Ð, of 1.09–1.21, and polymyrcene volume fractions of 30–64% are accessible. Matrix assisted laser desorption ionization‐time of flight mass spectrometry measurements reveal the temperature‐controlled polymyrcene block formation, while both transmission electron microscopy and small‐angle X‐ray scattering measurements prove the presence of clearly microphase‐separated, long range‐ordered domains in the block copolymers. The temperature‐controlled one‐pot anionic block copolymerization approach may be general for other terpene‐diene monomers.
Visual Ozone Sensor: Structural Color Change of Pendant Selenium‐Containing Maleimide Polymers via Oxidation
Authors: Li Qilong , Liu Shaoxiang , Li Jiajia , Pan Xiangqiang , Zhu Jian , Zhu Xiulin 
Published: October 2020

AbstractPendant selenium‐containing maleimide polymers with different selenium contents are synthesized via a radical copolymerization of styrene and N‐butylmaleimide phenyl selenide. The polymer structures are characterized by nuclear magnetic resonance, gel permeation chromatography, Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, and scanning electron microscopy with an energy‐dispersive spectrometer, which results in the desired structures and selenium contents. The refractive indices of the polymers, which change as a function of different contents of selenium and oxidative stimuli by H2O2 or O3, are investigated. Finally, a photonic crystal (PC) is prepared based on the selenium‐containing polymer. The visible color changes of the PC are investigated as a function of different concentrations and contact times of O3.
Photoelectrochemical (PEC) Investigation of Ga‐Doped MoBi2Se5 Thin Films Deposited by Arrested Precipitation Technique
Authors: Patil Satishkumar V. , Ghanwat Vishvanath B. , Pawar Neeta B. , Bhosale Popatrao N. 
Published: October 2020

AbstractNanocrystalline Ga‐doped molybdenum bismuth selenide thin films have been deposited onto amorphous and fluorine‐doped tin oxide (FTO) coated glass substrate using arrested precipitation technique (APT), which is based on self‐organized growth process. Deposited thin films of MoBi(2‐x)GaxSe5 are characterized by X‐ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), and X‐ray photoelectron spectroscopy (XPS). XRD analysis shows mixed phase type crystal structure of MoBi(2‐x)GaxSe5 thin films. SEM analysis shows that closely packed grains provide a pinhole‐free morphology. The average surface roughness of as deposited MoBi(2‐x)GaxSe5 (x = 0.0, 0.04, and 0.10) thin films obtained from AFM images is 5.04 nm μm‐2. In TEM analysis, lattice plane (015), (103), and (103) are observed in SAED pattern, which confirms mixed phase type crystal structure of MoBi(2‐x)GaxSe5 thin films. The binding energy, elemental composition, and surface nature of MoBi(2‐x)GaxSe5 thin film is confirmed by using XPS. The photoelectrochemical (PEC) investigations are carried out using cell configuration p‐MoBi(2‐x)GaxSe5(FTO)/0.5M (Na2Sx‐ NaOH‐S)/counter electrode. After Ga doping, MoBi2Se5 thin film electrodes exhibit photoactivity in sulphide/polysulphide electrolyte toward positive polarity (p‐type behavior). On the addition of gallium, power conversion efficiency of MoxBi(2‐x)GaxSe5 thin films decreases from 0.456 to 0.171%.
Superhydrophobic PU Sponge Modified by Hydrophobic Silica NPs—Polystyrene Nanocomposite for Oil–Water Separation
Authors: Sutar Rajaram S. , Salunkhe Revati C. , Latthe Sanjay S. , Kodag Vishnu S. , Shewale Poonam M. , Shinde Shital R. , Sajjan M. B. , Karennavar M. H. , Sadasivuni Kishor Kumar , Mohite Santosh V. , Liu Shanhu , Xing Ruimin 
Published: October 2020

AbstractIn this study, the hydrophobic silica nanoparticles (NPs) are synthesized by simple sol–gel processing of polymethylhydrosiloxane (PMHS). The nanocomposite solution is prepared by adding hydrophobic silica NPs in polystyrene solution and applied on the skeleton of polyurethane sponge by simple immersion‐drying process. The as‐prepared sponges exhibited superhydrophobic property with water contact angle 161° and oil contact angle nearly 0° and can separate oil from oil–water and oil–muddy water mixture. The superhydrophobic sponge has sustainable anti‐wetting property under cross sectional cutting, pressing and twisting, and different pH environment. Such superhydrophobic sponge is suitable for practical application on a large scale.
Oil–Water Separation by ZnO‐Based Superhydrophobic PU Sponges
Authors: Sutar Rajaram S. , Mane Manisha S. , Latthe Sanjay S. , Pawar P. G. , Kumbhar Sarita S. , Nerle Uma V. , Mote U. E. , Bhosale J. L. , Kokare B. N. , Sadasivuni Kishor Kumar , Liu Shanhu , Xing Ruimin 
Published: October 2020

AbstractContinuous oil–water separation is not only an important topic for scientific research but also for practical applications to clean oil from industrial oily wastewater and oil‐spill pollution. In this work, polyurethane sponges are coated by ZnO using dip coating technique. ZnO‐coated sponges are modified by stearic acid to achieve superhydrophobicity. The ZnO‐coated sponges exhibit water contact angle ≈165° and oil contact angle ≈0°. The prepared superhydrophobic sponge is sustained in oil–water separation and in separation of oil–hot water mixture. Also the wetting properties of the sponge are stable in mechanical test like cutting and twisting. Stearic acid modified ZnO‐coated sponge holds good promise for oil‐spill cleanup as well as oil/water separation from harsh environments.
Facile Synthesis and Characterization of CdO‐ZnO Nanocomposite for Gas Sensor
Authors: Pakhare Keshav S. , Sargar Balasaheb M. , Mane Raghunath K. , Wategaonkar Sandeep B. 
Published: October 2020

AbstractThe pure CdO, pure ZnO, and CdO‐ZnO composite films are successfully prepared by simple chemical bath deposition (CBD) method. XRD reveals a polycrystalline nature of CdO‐ZnO nanocomposite. The SEM micrograph depicts porous web network like morphology of CdO‐ZnO nanocomposite and EDX shows good agreement of elements. CdO‐ZnO nanocomposite shows maximum response of 29.11% under optimum operating temperature of 275˚C at 24 ppm ethanol gas concentration.
Superhydrophobic Nanocomposite Coatings of Hydrophobic Silica NPs and Poly(methyl methacrylate) with Notable Self‐Cleaning Ability
Authors: Sutar Rajaram S. , Gaikwad Sima S. , Latthe Sanjay S. , Kodag Vishnu S. , Deshmukh S. B. , Saptal L. P. , Kulal S. R. , Bhosale Appasaheb K. 
Published: October 2020

AbstractThe present paper describes a facile and inexpensive dip coating method for preparation of hierarchical superhydrophobic nanocomposite coating. The hydrophobic silica nanoparticles (NPs) are synthesized via sol–gel technique. The superhydrophobicity of the coating is controlled by adjusting the concentration of silica NPs in poly (methyl methacrylate) (PMMA). After optimization, the coating exhibited water contact angle of 165° and sliding angle of 4°. The surface morphology of the superhydrophobic coatings revealed hierarchical rough structure formed on the glass substrate, which enable air trapping. The prepared superhydrophobic coating showed wetting stability under water jet impact with notable self‐cleaning performance. The mechanical stability of the superhydrophobic coating is studied using sandpaper abrasion and adhesive tape peeling test. Such highly non‐wettable and self‐cleaning coatings have potential in various practical applications.
Effect of Sintering Temperature on Structural and Morphological Properties of Mn‐Substituted Lithium Ferrite
Authors: Patil Rajendra P. , Nikam Prashant N. , Patil Sarjerao B. , Dhokale Ramdas K. , Sawant Vijay S. , Shelke Satish B. 
Published: October 2020

AbstractManganese‐substituted lithium ferrite is synthesized by sol–gel method. The sample is sintered at different temperatures in air. Their structural and morphological properties are studied by X‐ray diffraction, scanning electron microscopy, and FT‐IR techniques. The X‐ray diffraction patterns reveal that all the samples consist of nanocrystalline single cubic phase structure. The morphological studies of synthesized nanocrystalline samples are obtained from the scanning electron microscopy technique. It can be seen that, the average grain size is increased significantly with increasing the sintering temperature. FT‐IR studies indicate that the spinel phase formation takes place at higher sintering temperature.
FT‐IR Spectra Analysis of PbxSr1‐x(NO3)2 Mixed Crystals for Optoelectronic Applications
Authors: Prathap Koyada , Rao E. Venkateshwar , Kumar P. M. , Hussain K. A. 
Published: October 2020

AbstractPb(NO3)2 and Sr(NO3)2 belong to a family of isomorphous alkaline earth nitrates with cubic structure. In view of ever growing applications of these nitrate crystals in stimulated Raman scattering laser shifters, optical filters and birefringence, numerous investigations are carried out on their various physical properties. Sr(NO3)2 crystal is a promising crystal for coupled intra‐cavity Raman laser. From Fourier transform infrared spectroscopy Raman spectra of these crystals in the near IR region 400–4000 cm−1 at room temperature, are investigated. The results indicate that lead shifts the Raman dominating peaks to the lower frequency and broaden the full width at half maximum. Furthermore, lead is anticipated to improve the properties of Sr(NO3)2.
Size and Shape Characteristics of Polystyrene and Poly(ethylene oxide) Star Polymer Melts Studied By Atomistic Simulations
Authors: Gkolfi Eirini , Bačová Petra , Harmandaris Vagelis 
Published: October 2020

AbstractHere, atomistic molecular dynamics simulations are used to address the effect of different chemistry on the morphology, size, and shape characteristics of star polymers. The role of chemical details and features such as packing or flexibility on the structural properties of star‐like polymer melts is probed. Two types of symmetric unentangled homopolymer stars, the first consisting of atactic polystyrene (PS) and the second one of poly(ethylene oxide) (PEO) are studied. The star functionality f (i.e., the number of arms) is varied from f = 1 (linear chains) up to f = 32 and the shape and size characteristics are reported as a function of this main variable. The results from the monodisperse star‐like polymers are confronted with the theoretical predictions and with previously published simulations studies employing generic bead‐spring coarse‐grained models, with the aim to fill the gap between the systems studied experimentally and computationally.
An inverse optimal approach to design of feedback control: Exploring analytical solutions for the Hamilton‐Jacobi‐Bellman equation
Authors: Komaee Arash 
Published: October 2020

SummaryDesign of feedback control by an optimal control approach relies on the solutions of the Hamilton‐Jacobi‐Bellman (HJB) equation, while this equation rarely admits analytical solutions for arbitrary choices of the performance measure. An inverse optimal feedback design approach is proposed here in which analytical solutions are explored for the HJB equation that optimize some meaningful, but not necessarily ideal, performance measure. Such performance measure is exclusively selected from a family of cost functionals with an intended structural constraint which inherently yields analytical solutions to the HJB equation. This family includes a set of free parameters which are exploited to construct cost functionals adequately representing the design requirements. The structural constraint imposed on this cost functional family indeed narrows down the scope of the proposed method; yet, it is shown by several examples that this method can successfully address certain classes of feedback design problems.