bluuebunny/crossref_metadata_2025_split huggingface dataset:
Filament inadequate wall apposition of the different ends of flow diverters in the abdominal aorta of rabbits
Authors: Wang Kuizhong , Jin Peng , Lu Peigang , Liu Qiang , Li Bo , Hao Zhenqiang 
Published: November 2019

Background Flow diverters (FDs) with flared ends (FEs) or straight ends (SEs) are used either alone or when overlapped to treat complex intracranial aneurysms. We evaluated filament inadequate wall apposition (IWA) of the FEs and SEs of FDs in vivo. Methods A total of 24 FDs with FEs and SEs were placed in an overlapping manner in the abdominal aortae of six rabbits (two sets per rabbit). Digital subtraction angiography was performed immediately after stent insertion and three months later. The anatomical and histopathological aortic features at FEs and SEs were evaluated. Results Angiography revealed no significant difference in terms of changes in arterial diameter between the FE and SE groups ( p = 0.877). Gross anatomical evaluation revealed IWA of the different ends of FDs but no thrombi or bleeding, showing that the metallic filaments were not in touch with the vessel wall and nor had they penetrated the vessel wall. The filaments’ IWA rates of FEs and SEs were 8.33% and 8.85%, respectively. The IWA rate at overlapping ends was lower than that at non-overlapping ends. The maximum neo-intimal thickness at FEs was greater than that at SEs (149.4 ± 48.9 and 98.6 ± 26.6 µm, respectively; p < 0.001). Conclusions Both the FEs and SEs of FDs can exhibit IMA. IWA events are reduced at the overlapped regions. On pathological evaluation, FEs increased neo-intimal thicknesses more than SEs did, but hyperplasia was minimal on angiography.
Patient-reported outcomes in multiple sclerosis: Validation of the Quality of Life in Neurological Disorders (Neuro-QoL™) short forms
Authors: Medina Luis D , Torres Stephanie , Alvarez Enrique , Valdez Brooke , Nair Kavita V 
Published: November 2019

Background Patient-reported outcome (PRO) measures have been shown to be effective for tracking treatment outcomes in multiple sclerosis (MS). However, collecting PROs as part of the clinical standard of care can be time-consuming and examination of their validity for use in an MS sample has been limited. Objective To determine the discriminant validity of the Quality of Life in Neurological Disorders (Neuro-QoL™) short forms in a real-world MS clinic population. Design/Methods Neuro-QoL is a series of questionnaires for tracking physical function, emotional/cognitive health, and social abilities in clinical populations. Neuro-QoL data from 902 MS patients were analyzed for psychometric properties and factor structure. Results Neuro-QoL demonstrated acceptable reliability in the moderate-to-good ranges. Moderate support for convergent validity was observed with other measures of MS quality of life, disease severity, and symptoms. However, results from a confirmatory factor analysis suggested poor model fit for most of the 12 domains tested. Conclusions These findings support the utility of some of the Neuro-QoL questionnaires in evaluating MS-related PROs. However, additional research may help abridge and strengthen these measures for use in this population.
Revision Anterior Cruciate Ligament (ACL) Reconstruction
Authors: Eufemio Edgar Michael T. 
Published: November 2019

The goal of an ACL reconstruction is to approximate the strength and characteristics of the native tissue. So, if the original ACL can rupture, it is logical to think that the reconstructed ligament can also tear. It is more difficult to deal with a revision case. Your decision what to do is dependent on what was done during the primary ACL reconstruction (ACLR). It is important to plan the surgery carefully and prepare for all possible scenarios intra-operatively. The three key questions you need to answer are the following? What was the GRAFT used? How were the TUNNELS made? What were the IMPLANTS used? It would be helpful to know who did the primary ACLR because you may be aware of some of the tendencies of that surgeon which may assist you in your surgery. There are seven situations that will be discussed: Bone-Patellar Tendon-Bone (BPTB) autograft using a trans-tibial technique Semitendinosus/Gracilis Tendon (ST/G) autograft using a trans-tibial technique With the advent of the medial portal technique, the position of the femoral tunnel changed dramatically. Five other circumstances arose from this: 3. ST/G autograft using endo-buttons 4. ST/G autograft using screws – femoral tunnel in ideal position 5. ST/G autograft using screws – femoral tunnel near where you want it to be 6. ST/G autograft using screws – femoral tunnel far from where you want it to be 7. Implant-less ACLR Always have a back-up plan when doing revisions. It is imperative that the rehabilitation program be delayed so as to give the graft more time to incorporate. Usually, twelve months before going back to sports is acceptable.
Revision of Failed-Posterior Cruciate Ligament (PCL) Reconstruction Due To Tibial Tunnel Misplacement: A Case Report
Authors: Lubis Andri , Kuncoro Mohamad Walid 
Published: November 2019

Objectives: Posterior cruciate ligament (PCL) reconstruction failure is a rare condition found. The failure caused by some factors, including improper graft tunnel placement. The proper tibial tunnel placement in PCL reconstruction is still controversial. To have an anatomical tunnel is essential to decrease the risk of failure. The use of PCL jig only to guide the direction of the tibial tunnel may not always give good results. Case presentation: We report a case of a 29-year-old male with a total rupture of ACL and PCL that underwent reconstruction for both ligaments. We found the failure of the PCL graft 2 years after the surgery was related to the tibial tunnel placement which was placed not in proper anatomical site. We performed revision PCL surgery with a transseptal portal technique to ensure the tibial tunnel is placed inappropriate position. Result: The cause of failure was associated with the miss position of the tibial tunnel. The tibial tunnel performed in previous surgery was too anterior than the anatomical footprint. This condition might be caused by a surgical technique that depending only on PCL jig to guide the tibial tunnel direction and location. We performed a transseptal portal technique to get better visualization on the posterior aspect of the knee to achieve the proper direction of the tibial tunnel. Conclusion: The use of PCL jig as the only tool for guiding tibial tunneling should be avoided. An additional tool such as a transseptal portal is required to ensure the proper anatomical tibial tunnel to achieve good PCL graft placement.
Arthroscopic Revision Bankart for Recurrent Anterior Shoulder Dislocation : Contributing Factor Analysis and a Case Report
Authors: Lubis Andri , Oktari Prima Rizky 
Published: November 2019

Objectives: Recurrent shoulder dislocation after arthroscopic Bankart repair (ABR) is still a matter of discussion. The recurrence rate varies between four to fourteen percent in the general population. Re-injury timing, large anatomical defect, and anchor techniques are the factors contributing to re-dislocation after ABR, yet there was still no consensual algorithm for Bankart revision. Case presentation: We presented a case of 27 years old woman with recurrent anterior dislocation after seven years of arthroscopic Bankart repair. Seven years ago, we did Bankart repair using a 2.8 mm fiber-wire anchor (FASTak® (Arthrex, Karsfield Germany)). Now we decided to do arthroscopic revision using all suture anchor technique (Y-Knot® Flex All-Suture Anchor, 1.3mm – One strand of #2 Hi-Fi® (Conmed, New York)). Results: From preoperative and intraoperative assessment, we found that the mechanism of injury, Hill’s-Sachs lesion, Bankart lesion and the number of anchors contributed to re-dislocation. Four months postoperative follow up showed that the patient could gain a normal range of movement. No early or late complications were observed. Conclusion: The success of arthroscopic Bankart repair greatly depended on the preoperative assessment. The arthroscopic revision will do many benefits to the patient since it is less invasive and not compromising the post-operative range of motion of the shoulder.
Receiving a Diagnosis of Pernicious Anemia: Exploring Experiences of Relationships With Health Professionals
Authors: Seage Catherine Heidi , Glover Emily , Mercer Jenny 
Published: November 2019

Objectives: Pernicious anemia (PA) is a chronic condition caused by vitamin B12 deficiency. This is a qualitative study using interpretative phenomenological analysis (IPA), which aimed to explore the patients lived experience of diagnosis and treatment. Methods: Eleven semistructured interviews were conducted in PA patients; these covered participants’ diagnostic and treatment journeys, the responses of others to their diagnosis, and the role health professionals have played in their medical care. Interviews were analyzed for recurrent themes using IPA. Results: Three superordinate themes were identified: “The struggle to achieve a diagnosis,” “The significance of a diagnosis,” and “Battling for sufficient treatment.” Participants were dissatisfied with their medical care due to diagnostic delay, insufficient treatment, and poor relationships with their health professionals. Many experienced, anticipated, and internalized stigma, which led to a reduced quality of life and withdrawal from the medical profession. Discussion: Participants’ interactions with their health professionals hindered their adaptation to their condition; this affected their psychological and physical well-being. Increased clinician awareness of stigma in the PA population symptoms and effective patient-centered communication is required.
Percutaneous posterior tibial nerve stimulation for the treatment of the overactive bladder in children: Is it effective?
Authors: Ibrahim Hisham , Shouman Ahmed Mahmoud , Ela Waseem , Ghoneima Waleed , Shoukry Ahmed Ismail , ElSheemy Mohammed , Morsi Hani , Badawy Hesham , Eissa Mohamed , Kotb Sameh 
Published: November 2019

Abstract
Background
To assess the efficacy and durability of percutaneous posterior tibial nerve stimulation for the treatment of the overactive bladder in children.

Results
Twenty patients with a mean age of 7 ± 3.3 years were included in the study. Subjective success was found in 12 patients (60%) who considered their situation to be better and requested to continue therapy to maintain the response which was considered as a therapeutic success, while eight patients (40%) patients chose not to continue therapy because they did not have symptomatic improvement.

Conclusion
Posterior tibial nerve stimulation is an effective, minimally invasive method for treatment and is easily applied to induce improvement in overactive bladder symptoms and objective urodynamic changes with negligible side effects.
The evolution of reproductive isolation in Daphnia
Authors: Chin Tiffany A. , Cáceres Carla E. , Cristescu Melania E. 
Published: November 2019

Abstract
Background
The process by which populations evolve to become new species involves the emergence of various reproductive isolating barriers (RIB). Despite major advancements in understanding this complex process, very little is known about the order in which RIBs evolve or their relative contribution to the total restriction of gene flow during various stages of speciation. This is mainly due to the difficulties of studying reproductive isolation during the early stages of species formation. This study examines ecological and non-ecological RIB within and between Daphnia pulex and Daphnia pulicaria, two recently diverged species that inhabit distinct habitats and exhibit an unusual level of intraspecific genetic subdivision.

Results
We find that while ecological prezygotic barriers are close to completion, none of the non-ecological barriers can restrict gene flow between D. pulex and D. pulicaria completely when acting alone. Surprisingly, we also identified high levels of postzygotic reproductive isolation in ‘conspecific’ interpopulation crosses of D. pulex.

Conclusions
While the ecological prezygotic barriers are prevalent during the mature stages of speciation, non-ecological barriers likely dominated the early stages of speciation. This finding indicates the importance of studying the very early stages of speciation and suggests the contribution of postzygotic isolation in initiating the process of speciation.
Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family
Authors: Adedipe Folukemi , Grubbs Nathaniel , Coates Brad , Wiegmman Brian , Lorenzen Marcé 
Published: November 2019

AbstractBackgroundThe western corn rootworm,Diabrotica virgifera virgifera, is a pervasive pest of maize in North America and Europe, which has adapted to current pest management strategies. In advance of an assembled and annotatedD. v. virgiferagenome, we developed transcriptomic resources to use in identifying candidate genes likely to be involved in the evolution of resistance, starting with members of the ATP-binding cassette (ABC) transporter family.ResultsIn this study, 65 putativeD. v. virgiferaABC (DvvABC) transporters were identified within a combined transcriptome assembly generated from embryonic, larval, adult male, and adult female RNA-sequence libraries. Phylogenetic analysis placed the deduced amino-acid sequences of theDvvABC transporters into eight subfamilies (A to H). To supplement our sequence data with functional analysis, we identified orthologs ofTribolium castaneumABC genes which had previously been shown to exhibit overt RNA interference (RNAi) phenotypes. We identified eight suchD. v. virgiferagenes, and found that they were functionally similar to theirT. castaneumcounterparts. Interestingly, depletion ofDvvABCB_39715andDvvABCG_3712transcripts in adult females produced detrimental reproductive and developmental phenotypes, demonstrating the potential of these genes as targets for RNAi-mediated insect control tactics.ConclusionsBy combining sequence data from four libraries covering three distinct life stages, we have produced a relatively comprehensive de novo transcriptome assembly forD. v. virgifera. Moreover, we have identified 65 members of the ABC transporter family and provided the first insights into the developmental and physiological roles of ABC transporters in this pest species.
N-acetyltransferase AAC(3)-I confers gentamicin resistance to Phytophthora palmivora and Phytophthora infestans
Authors: Evangelisti Edouard , Yunusov Temur , Shenhav Liron , Schornack Sebastian 
Published: November 2019

Abstract
Background
Oomycetes are pathogens of mammals, fish, insects and plants, and the potato late blight agent Phytophthora infestans and the oil palm and cocoa infecting pathogen Phytophthora palmivora cause economically impacting diseases on a wide range of crop plants. Increasing genomic and transcriptomic resources and recent advances in oomycete biology demand new strategies for genetic modification of oomycetes. Most oomycete transformation procedures rely on geneticin-based selection of transgenic strains.

Results
We established N-acetyltransferase AAC(3)-I as a gentamicin-based selectable marker for oomycete transformation without interference with existing geneticin resistance. Strains carrying gentamicin resistance are fully infectious in plants. We further demonstrate the usefulness of this new antibiotic selection to super-transform well-characterized, already fluorescently-labelled P. palmivora strains and provide a comprehensive protocol for maintenance and zoospore electro-transformation of Phytophthora strains to aid in plant-pathogen research.

Conclusions
N-acetyltransferase AAC(3)-I is functional in Phytophthora oomycetes. In addition, the substrate specificity of the AAC(3)-I enzyme allows for re-transformation of geneticin-resistant strains. Our findings and resources widen the possibilities to study oomycete cell biology and plant-oomycete interactions.
A metabolomics characterisation of natural variation in the resistance of cassava to whitefly
Authors: Perez-Fons Laura , Bohorquez-Chaux Adriana , Irigoyen Maria L. , Garceau Danielle C. , Morreel Kris , Boerjan Wout , Walling Linda L. , Becerra Lopez-Lavalle Luis Augusto , Fraser Paul D. 
Published: November 2019

Abstract
Background
Cassava whitefly outbreaks were initially reported in East and Central Africa cassava (Manihot esculenta Crantz) growing regions in the 1990’s and have now spread to other geographical locations, becoming a global pest severely affecting farmers and smallholder income. Whiteflies impact plant yield via feeding and vectoring cassava mosaic and brown streak viruses, making roots unsuitable for food or trading. Deployment of virus resistant varieties has had little impact on whitefly populations and therefore development of whitefly resistant varieties is also necessary as part of integrated pest management strategies. Suitable sources of whitefly resistance exist in germplasm collections that require further characterization to facilitate and assist breeding programs.

Results
In the present work, a hierarchical metabolomics approach has been employed to investigate the underlying biochemical mechanisms associated with whitefly resistance by comparing two naturally occurring accessions of cassava, one susceptible and one resistant to whitefly. Quantitative differences between genotypes detected at pre-infestation stages were consistently observed at each time point throughout the course of the whitefly infestation. This prevalent differential feature suggests that inherent genotypic differences override the response induced by the presence of whitefly and that they are directly linked with the phenotype observed. The most significant quantitative changes relating to whitefly susceptibility were linked to the phenylpropanoid super-pathway and its linked sub-pathways: monolignol, flavonoid and lignan biosynthesis. These findings suggest that the lignification process in the susceptible variety is less active, as the susceptible accession deposits less lignin and accumulates monolignol intermediates and derivatives thereof, differences that are maintained during the time-course of the infestation.

Conclusions
Resistance mechanism associated to the cassava whitefly-resistant accession ECU72 is an antixenosis strategy based on reinforcement of cell walls. Both resistant and susceptible accessions respond differently to whitefly attack at biochemical level, but the inherent metabolic differences are directly linked to the resistance phenotype rather than an induced response in the plant.
PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles
Authors: Liu Shun , Hu Zi-Min , Zhang Quansheng , Yang Xiaoqi , Critchley Alan T. , Duan Delin 
Published: November 2019

Abstract
Background
Intermittent dehydration caused by tidal changes is one of the most important abiotic factors that intertidal seaweeds must cope with in order to retain normal growth and reproduction. However, the underlying molecular mechanisms for the adaptation of red seaweeds to repeated dehydration-rehydration cycles remain poorly understood.

Results
We chose the red seaweed Gloiopeltis furcata as a model and simulated natural tidal changes with two consecutive dehydration-rehydration cycles occurring over 24 h in order to gain insight into key molecular pathways and regulation of genes which are associated with dehydration tolerance. Transcription sequencing assembled 32,681 uni-genes (GC content = 55.32%), of which 12,813 were annotated. Weighted gene co-expression network analysis (WGCNA) divided all transcripts into 20 modules, with Coral2 identified as the key module anchoring dehydration-induced genes. Pathways enriched analysis indicated that the ubiquitin-mediated proteolysis pathway (UPP) and phosphatidylinositol (PI) signaling system were crucial for a successful response in G. furcata. Network-establishing and quantitative reverse transcription PCR (qRT-PCR) suggested that genes encoding ubiquitin-protein ligase E3 (E3–1), SUMO-activating enzyme sub-unit 2 (SAE2), calmodulin (CaM) and inositol-1,3,4-trisphosphate 5/6-kinase (ITPK) were the hub genes which responded positively to two successive dehydration treatments. Network-based interactions with hub genes indicated that transcription factor (e.g. TFIID), RNA modification (e.g. DEAH) and osmotic adjustment (e.g. MIP, ABC1, Bam1) were related to these two pathways.

Conclusions
RNA sequencing-based evidence from G. furcata enriched the informational database for intertidal red seaweeds which face periodic dehydration stress during the low tide period. This provided insights into an increased understanding of how ubiquitin-mediated proteolysis and the phosphatidylinositol signaling system help seaweeds responding to dehydration-rehydration cycles.
Single-molecule real-time sequencing facilitates the analysis of transcripts and splice isoforms of anthers in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Authors: Tan Chong , Liu Hongxin , Ren Jie , Ye Xueling , Feng Hui , Liu Zhiyong 
Published: November 2019

Abstract
Background
Anther development has been extensively studied at the transcriptional level, but a systematic analysis of full-length transcripts on a genome-wide scale has not yet been published. Here, the Pacific Biosciences (PacBio) Sequel platform and next-generation sequencing (NGS) technology were combined to generate full-length sequences and completed structures of transcripts in anthers of Chinese cabbage.

Results
Using single-molecule real-time sequencing (SMRT), a total of 1,098,119 circular consensus sequences (CCSs) were generated with a mean length of 2664 bp. More than 75% of the CCSs were considered full-length non-chimeric (FLNC) reads. After error correction, 725,731 high-quality FLNC reads were estimated to carry 51,501 isoforms from 19,503 loci, consisting of 38,992 novel isoforms from known genes and 3691 novel isoforms from novel genes. Of the novel isoforms, we identified 407 long non-coding RNAs (lncRNAs) and 37,549 open reading frames (ORFs). Furthermore, a total of 453,270 alternative splicing (AS) events were identified and the majority of AS models in anther were determined to be approximate exon skipping (XSKIP) events. Of the key genes regulated during anther development, AS events were mainly identified in the genes SERK1, CALS5, NEF1, and CESA1/3. Additionally, we identified 104 fusion transcripts and 5806 genes that had alternative polyadenylation (APA).

Conclusions
Our work demonstrated the transcriptome diversity and complexity of anther development in Chinese cabbage. The findings provide a basis for further genome annotation and transcriptome research in Chinese cabbage.