bluuebunny/crossref_metadata_2025_split huggingface dataset:
Willingness to perform induced abortion and associated factors among graduating Midwifery, Medical, Nursing, and Public health officer students of University of Gondar, Northwest Ethiopia: Institution based Cross sectional study
Authors: Enyew Mihretu Molla 
Published: October 2020

Abstract
Background: In developing countries, abortion is often unsafe and a signiﬁcant cause of maternal morbidity and mortality accounting for about 8% (4.7%–13.2%) of maternal mortality worldwide. Internationally, safe abortion services are recognized as reducing maternal mortality, and liberalized abortion laws are associated with reduced mortality resulting from unsafe abortion procedures. However, health care providers have moral, social and gender-based reservations that affects their willingness towards providing induced abortion services. The purpose of this study was to assess willingness to perform induced abortion and associated factors among graduating Midwifery, Medical, Nursing, and Public health officer students of University of Gondar. Methods: Institution based cross sectional study was conducted from March 29 to May 30, 2019. All graduating students available during data collection period were considered as study population. Stratified simple random sampling technique was used to select 424 study participants. Pre tested, semi- structured, self-administered questionnaire was used to collect data. Data analysis was done using SPSS version 20. Ethical clearance was obtained from School of midwifery under the delegation of institutional review board of university of Gondar.Results: 290 students out of 424 students were willing to perform induced abortion for indications supported by Ethiopian abortion law, making a proportion of 68.4% (95%Cl: 64.2, 72.9). Sex (Being male (AOR = 4.89, 95%CI: 3.02, 7.89)), religion (being orthodox than protestant (AOR = 10.41, 95%CI: 3.02, 21.57)), being Muslim than protestant (AOR = 5.73, 95%CI: 1.37, 15.92)) and having once or less a week religious attendance (AOR=2.00, 95% CI: 1.20, 3.34) were factors associated with willingness towards performing induced abortion.Conclusions: According to this study willingness of students towards providing induced abortion services was good. However female students, protestant followers and those students with more than once a week religious attendance should be encouraged to support women’s access to induced abortion services by referring them to other health care professionals willing to provide induced abortion services.
CT scan blend signs are not associated with poor outcome in patients with ICH who undergo stereotactic minimally invasive surgery
Authors: Yang Xu , Zhu Yan , Zhang Linshan , Wang Likun , Mao Yuanhong , Li Yinghui , Luo Jingbiao , Wu Guofeng 
Published: October 2020

Abstract
Background: The initial CT blend sign has been used as an imaging marker to predict haematoma expansion and poor outcomes in patients with a small volume intracerebral haemorrhage (ICH). However, the relationship between the blend sign and outcomes remains elusive. The present study aimed to retrospectively measure the impact of initial CT blend signs on short-term outcomes in patients with hypertensive ICH who underwent stereotactic minimally invasive surgery (sMIS). Methods: We enrolled 242 patients with spontaneous ICH. Based on the initial CT features, the patients were assigned to a blend sign group (91 patients) or a non-blend sign (control) group (151 patients). The NIHSS, GCS and mRS were used to measure the efficacy of the sMIS. The rates of severe pulmonary infection and cardiac complications were also compared between the two groups. Results: No significant differences in NIHSS and GCS at one week or two weeks after surgery were observed between the two groups. The proportion of patients with good outcomes during follow-up was not different in the two groups. Both groups displayed good functional outcomes relative to the state at admission. The rate of re-haemorrhaging increased in the blend sign group. No significant differences in severe pulmonary infections and cardiac complications were noted between the two groups. Conclusions: The initial CT blend sign was not associated with poor outcome in patients with hypertensive ICH who underwent sMIS. ICH patients with CT blend signs should undergo sMIS if they are suitable candidates for surgery.
Comparing Zirconia to Anterior Strip Crowns in Primary Anterior Teeth in children, A Randomized Clinical Trial
Authors: Alaki Sumer M , Abdulhadi Bashaer S , AbdelBaki Medhat A , Al-Alamoudi Najla M 
Published: October 2020

Abstract
Background: Providing restorations to anterior teeth in children is a challenging task due to the need for high esthetics, strength, and durability.Aim: this study was done to compare prefabricated primary zirconia with resin composite strip crowns on primary maxillary central and lateral incisors with regards to gingival health, plaque accumulation, recurrent caries, restoration failure, and opposing teeth wear over a period of 3, 6 and 12 months.Methods: Children attending the King Abdulaziz University, Faculty of Dentistry (KAUFD) clinics who needed restorations were screened for inclusion criteria. A total of 120 teeth were treated; 60 with zirconia and 60 with strip crowns. Randomization was done by simple random allocation using SPSS software version 20.0 (Armonk, NY; IBM Corp.). A simple descriptive statistic was used for analysis by Wilcoxon Signed-Rank test and Chi-square test. Level of significance was set at (α = 0.05) and level of confidence at (95%).Results: Zirconia crowns showed significantly less gingival bleeding at the 3- and 6-months follow up periods (p<0.006, p<0.001; respectively), less plaque accumulation at all follow up visits (p<0.001), no restoration failure (p<0.001), but more wear to opposing teeth (p<0.02). No significant difference was found between the two crowns with regards to recurrent caries (p<0.135).Conclusion: Based on our data we conclude that overtime teeth covered with zirconia crowns show better gingival health and less bleeding, plaque accumulation as well as less loss of material. On the other hand, zirconia can cause more loss of opposing tooth structure.Trial Registration: The presented research was registered retrospectively at ClinicalTrials.gov under registration number NCT03184012.
Prevalence of Tumor necrosis factor alpha inducing protein (tipα) gene of Helicobacter pylori and its association with Upper Gastrointestinal Diseases in India
Authors: Mahant Shweta , Mehra Shubham , Chhawchharia Ayushi , Karmakar Bipul Chandra , Paul Sangita , Mukhopadhyay Asish Kumar , Bose Sudeep , Das Kunal , Das Rajashree 
Published: October 2020

Abstract
Background: Helicobacter pylori (H. pylori) is known to cause several gastro-duodenal diseases including chronic Gastritis, Peptic Ulcer disease and Gastric Cancer. Virulent genes of H. pylori like cagA, vacA are known to be responsible for the disease pathogenesis. But these virulence genes are not always found to be associated with disease outcome in all populations around the world. Relationship of H. pylori with Gastro Esophageal Reflux Diseases is controversial and uncertain. Tumor necrosis factor alpha inducing protein tipα is a new discovered virulent gene of H. pylori and is an inducer of certain cytokines and chemokines that are responsible for causing stomach cancer. Therefore, we conducted a study which aims to find the prevalence of tipα gene in the Indian population and its association with H. pylori related upper gastrointestinal diseases.Results: 267 clinical H. pylori isolates are included in our study for finding the prevalence of tipα gene and its association with cagA and vacA gene using PCR assay. The current study shows the prevalence rate of tipα gene in the Indian population to be 59.9%. Our study has found a significant association (p < 0.05) of tipα gene with Non Ulcer Dyspepsia (NUD) and also an association of cagA and vacA s1m1 with Gastritis and Duodenal Ulcer.Conclusion: Our study demonstrates for the first time the presence of tipα as virulent factor of H. pylori strain in Indian population isolated from patients suffering from upper gastrointestinal diseases. Further, tipα is significantly associated with NUD but not with other upper gastrointestinal diseases in India.
Integrating expert opinions with clinical trial data to analyse low-powered subgroup analyses: a Bayesian analysis of the VeRDiCT trial
Authors: Thirard Russell , Ascione Raimondo , Blazeby Jane , Rogers Chris A 
Published: October 2020

Abstract
Background Typically, subgroup analyses in clinical trials are conducted by comparing the intervention effect in each subgroup by means of an interaction test. However, trials are rarely, if ever, adequately powered for interaction tests, so clinically important interactions may go undetected. We discuss the application of Bayesian methods by using expert opinions alongside the trial data. We applied this methodology to the VeRDiCT trial investigating the effect of preoperative volume replacement therapy (VRT) versus no VRT (usual care) in diabetic patients undergoing cardiac surgery. Two subgroup effects were of clinical interest, a) preoperative renal failure and b) preoperative type of antidiabetic medication. Methods Clinical experts were identified within the VeRDiCT trial centre in the UK. A questionnaire was designed to elicit opinions on the impact of VRT on the primary outcome of time from surgery until medically fit for hospital discharge, in the different subgroups. Prior beliefs of the subgroup effect of VRT were elicited face-to-face using two unconditional and one conditional questions per subgroup analysis. The robustness of results to the ‘community of priors’ was assessed. The community of priors was built using the expert priors for the mean average treatment effect, the interaction effect or both in a Bayesian Cox proportional hazards model implemented in the STAN software in R. Results Expert opinions were obtained from 7 clinicians (6 cardiac surgeons and 1 cardiac anaesthetist). Participating experts believed VRT could reduce the length of recovery compared to usual care and the greatest benefit was expected in the subgroups with the more severe comorbidity. The Bayesian posterior estimates were more precise compared to the frequentist maximum likelihood estimate and were shifted toward the overall mean treatment effect. Conclusions In the VeRDiCT trial, the Bayesian analysis did not provide evidence of a difference in treatment effect across subgroups. However, this approach increased the precision of the estimated subgroup effects and produced more stable treatment effect point estimates than the frequentist approach. Trial methodologists are encouraged to prospectively consider Bayesian subgroup analyses when low-powered interaction tests are planned.Trial registration ISRCTN, ISRCTN02159606. Registered 29th October 2008.
Cerebrospinal fluid analysis in 108 patients with progressive multifocal leukoencephalopathy
Authors: Möhn Nora , Yi Luo , Skripuletz Thomas , Schwenkenbecher Philipp , Ladwig Anne , Warnke Clemens , Meuth Sven G. , Wiendl Heinz , Gross Catharina C. , Schröder Christoph , Haghikia Aiden , Stangel Martin 
Published: October 2020

Abstract
Background: Progressive multifocal leukoencephalopathy (PML) is caused by an opportunistic infection with JC polyoma virus (JCPyV) and mainly affects immunocompromised patients. It leads to pronounced demyelination of the central nervous system (CNS) resulting in severe disability or even death. Detection of JCPyV DNA in the cerebrospinal fluid (CSF) is usually accepted as proof for the diagnosis of PML. Routine CSF parameters, like CSF cell count, protein concentration, Qalbumin, or intrathecal immunoglobulin synthesis are mostly considered normal. However, this has not been investigated systematically. Methods: We analyzed routine CSF parameters in a cohort of 108 PML patients that were treated at four different neurological centers in Germany. The patients exhibited different underlying conditions with natalizumab-treated multiple sclerosis (n=54) and human immunodeficiency virus (HIV)-infection (n=25) being the most frequent. The data were collected at the respective centers in accordance with local requirements and then jointly analyzed. The total PML cohort was compared with a control group of patients with normal pressure hydrocephalus (NPH) and idiopathic intracranial hypertension (IIH). Multiple sclerosis and HIV patients were additionally compared with their own non-PML control groups. Results: The PML group showed an elevated cell count (p<0.001) compared to the control group, however, this effect was mainly driven by HIV-PML patients. This subgroup also demonstrated a significantly higher proportion of patients with a disturbed blood-CSF-barrier function. Conclusions: This comprehensive, retrospective study on CSF diagnostic analysis in PML patients provides insight into the CSF of those patients. It demonstrates that CSF composition in PML patients may be specific for the underlying condition that predisposes for the development of PML and thus data have to be interpreted in this context.
Integrative analysis of long non-coding RNA (lncRNA) and mRNA expression in TLR4-primed MSCs of ankylosing spondylitis
Authors: Li Yuxi , Li Ming , Liu Ting , Huang Jiajun , Liang Yuwei , Huang Junshen , Liu Xiangge , Cheng Ziying , Lu Shixin , Shen Huiyong , Huang Lin 
Published: October 2020

Abstract
Background: Ankylosing spondylitis (AS) is a chronic autoimmune disease, and the precise pathogenesis is largely unknown at present. Our previous study found that the expression of toll-like receptor 4 (TLR4) of mesenchymal stem cells from AS patients (AS-MSCs) was reduced and activation of TLR4 by lipopolysaccharide (LPS) could enhance the immunoregulatory ability of AS-MSCs. However, the potential mechanism by which TLR4 affect the immunoregulatory function of AS-MSCs remains unclear. Objective: The goal of this study was to explore the expression profiles and functional networks of lncRNAs and mRNAs in TLR4-primed AS-MSCs and to clarify the potential mechanisms by which TLR4-primed AS-MSCs exert immunoregulatory effects.Methods: Immunoregulatory effects of MSCs were determined after TLR4 activation. Then, the differentially expressed (DE) long non-coding RNAs (lncRNA) and messenger RNAs (mRNA) between AS-MSCs and TLR4-primed AS-MSCs (stimulated by LPS) were identified through high-throughput sequencing followed by qRT-PCR confirmation. Finally, bioinformatic analyses were performed to identify the critical biological functions, signalling pathways and associated functional networks involved in the TLR4-primed immunoregulatory function of AS-MSCs.Results: A total of 147 DE lncRNAs and 698 DE mRNAs were identified between TLR4-primed AS-MSCs and unstimulated AS-MSCs. Of total, 107 lncRNAs were upregulated and 40 were downregulated (fold change ≥2, P <0.05), while 504 mRNAs upregulated and 194 downregulated (fold change ≥2, P <0.05). 5 lncRNAs and 5 mRNAs with largest fold changes were respectively verified by qRT-PCR. GO and KEGG analysis demonstrated that the DE mRNAs and lncRNAs were highly associated with the inflammatory response, such as NOD-like receptor (NLR) signalling pathway, the TNF signalling pathway and the NF-kappa B signalling pathway. Cis-regulation prediction revealed 8 novel lncRNAs while trans-regulation prediction revealed 15 lncRNAs, respectively. 8 core pairs of LncRNA and target mRNA in the lncRNA-TF-mRNA network were: PACERR-PTGS2, LOC105378085-SOD2, LOC107986655-HIVEP2, MICB-DT-MICB, LOC105373925-SP140L, LOC107984251-IFIT5, LOC112268267-GBP2 and LOC101926887-IFIT3, respectively.Conclusion: In AS, TLR4 activation can enhance the immunoregulation ability of MSCs. Eight core pairs of lncRNA and target mRNA have been found in TLR4-primed AS-MSCs, which could contribute to elucidate the potential mechanism of immunoregulatory dysfunction of AS-MSCs.
In silico Analysis of Epitope-Based CadF Vaccine Design against&nbsp;Campylobacter&nbsp;jejuni
Authors: Naseri Mona Moballegh , Shams Saeed , Naseri Mohammad Moballegh , Bakhshi Bita 
Published: October 2020

Abstract
Objective: Vaccination is an important strategy for the eradication of infectious diseases. CadF protein of Campylobacter jejuni is one of the important factors in the pathogenesis of this bacterium. The purpose of this work was to perform a bioinformatics study to identify an epitope-based CadF vaccine, as a subunit vaccine. Full protein sequences of CadF were extracted from the NCBI and UniProt databases and subjected to in silico evaluations, including sequence analysis, allergenicity, antigenicity, epitope conservancy, and molecular docking assessments done by different servers. Results: The results showed that CadF was a highly conserved protein belonging to the outer member proteins superfamily. Among the evaluated epitopes, LSDSLALRL was identified as an antigenic and non-allergenic peptide with a suitable structure for vaccine development. It was also able to stimulate both T and B cells. This 9-mer peptide was located in 136-144 segment of CadF protein and interacted with both HLA-A 0101 and HLA-DRB1 0101 alleles. Overall, the obtained theoretical results showed that CadF protein could be used for designing and evaluating a new effective vaccine against C. jejuni.
lncRNA F11-AS1 Suppresses Glioma by Regulation miRNA-3146/PTEN
Authors: Qin Hao , Yang Qixia , Wang Tiantian , Liu Long , Di Chao , Zhuang Qiang , Xiao Longhai , Yin Hang 
Published: October 2020

Abstract
BACKGROUND: LncRNAs play key roles in glioma development, however, lncRNA F11-AS1’s effects has been unclearly in glioma.OBJECTIVE: F11-AS1 is an anti-tumor factor in glioma has been clearly until now.METHODS: Evaluating F11-AS1 expression by ISH in difference tissues and analysis the correlation with glioma prognosis in clinical. Using U87 and U251 cell to discuss lncRNA F11-AS1’s effect in glioma cancer cell biological activities by MTT, flow cytometry, transwell and wound healing assay. And measuring the relative miRNA and gene expressions by RT-qPCR and relative proteins expressions by WB assay. In next step, discuss the miR-3146 in F11-AS1 depressing cancer cell biological activities.RESULTS: The authors observed in this study that F11-AS1 was down-regulation in glioma and negatively correlated with miRNA-3146. Servival analysis showed that low level of F11-AS1 predicted poor survival. In glioma cells, F11-AS1 knockdown led to up-regulate, whereas F11-AS1 overexpression led to down-regulated miRNA-3146. Analysis of cell biological activities showed that F11-AS1 overexpression had effects to suppress glioma cell lines biological activities.CONCLUSION: Therefore, F11-AS1 down-regulates miRNA-3146 to depress cell biological activities in glioma.
Endoscopic submucosal dissection for gastric tube carcinoma after esophagectomy contributes to long-term outcomes: a retrospective case series
Authors: Hashimoto Satoru , Sato Hiroki , Mizuno Ken-ichi , Takahashi Kazuya , Takatsuna Masafumi , Yokoyama Junji , Ichikawa Hiroshi , Takeuchi Manabu , Kobayashi Masaaki , Terai Shuji 
Published: October 2020

Abstract
Background: Gastric tube carcinoma (GTC) after esophagectomy has been observed increasingly in recent years after advanced treatment of esophageal carcinoma. Surgical removal of the reconstructed gastric tube is not usually preferred; however, endoscopic submucosal dissection (ESD) is greatly anticipated to be a promising option. This study investigated the efficacy and safety of ESD for GTC. Methods: This study was designed based on a retrospective survey performed at our single center between 2003 and 2018. Patients with GTC were treated with ESD and followed-up according to the policy of our institution. The curability of ESD was evaluated histologically according to the Japanese gastric cancer treatment guidelines. Patient characteristics, outcomes of ESD procedures, and long-term outcomes were analyzed. Results: A total of 31 patients (2 females; median age, 73 years) with 45 GTC cases were treated with ESD. The mean period from esophagectomy to the diagnosis of GTC was 10.6 years. The only adverse event due to ESD was bleeding (2 patients: 6.5%). No fatal events, such as perforation, occurred. The rates of complete resections and curative resections were 80.6% and 48.4%, respectively. The 3-year and 5-year overall survival rates were 67.6% and 47.7%, respectively. The 3-year and 5-year disease-specific survival rates were 100% and 92.9%, respectively. One patient died of GTC and 14 patients died of other diseases, including five primary carcinomas. Conclusions: ESD for GTC can be a safe treatment and contribute to positive long-term outcomes. Long-term regular gastroscopy is required for early detection. Moreover, additional care should be given to GTC patients who are at high risk for other primary carcinomas or comorbidities.
Paternity in male kidney transplant recipients: A French national survey, the PATeRNAL study.
Authors: Boyer Annabel , Lobbedez Thierry , Ouethrani Mohamed , Lecouf Angélique Thuillier , Bouvier Nicolas , Châtelet Valérie , de Ligny Bruno Hurault 
Published: October 2020

Abstract
Background: There is concern about the impact of immunosuppressive agents taken by male kidney transplant (KT) recipients on the risk of foetal malformations. The aim of our survey was to estimate the paternity rate and the outcomes of pregnancies fathered by kidney transplanted males. Methods: This survey analysed 1332 male KT recipients older than 18 years, followed in 13 centres in France. A self-reported questionnaire was used to collect data on the patients, treatments at the time of conception and the pregnancy outcomes.Results: The study included data on 349 children from 404 pregnancies fathered by 232 male KT recipients. The paternity rate was 17% (95% CI [15–20]). There were 37 (9%, 95% CI [7–12]) spontaneous abortions, 12 (3%, 95% CI [2–5]) therapeutic abortions, 2 (0.5%, 95% CI [0.1–1]) still births, and 13 (4%, 95% CI [2–6]) malformations reported. Compared to the general population, there was no difference in the proportion of congenital malformations nor unwanted outcomes whether the father was exposed or not to immunosuppressive agents.Conclusions: This survey does not provide any warning signal that pregnancies fathered by male patients exposed to immunosuppressive agents, notably the debated MMF/MPA, have more complications than pregnancies in the general population.