bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bronchial Thermoplasty Reduces Ventilation Heterogeneity Measured by Multiple Breath Nitrogen Washout
Authors: Langton David , Bennetts Kim , Thien Francis , Plummer Virginia , Noble Peter B 
Published: August 2020

Abstract
Background Despite demonstrated symptomatic benefit from bronchial thermoplasty (BT), the underlying pathophysiological benefits have been uncertain. The purpose of the present study was to relate clinical benefit after BT to changes in lung physiology, focusing on ventilation homogeneity assessed using multiple breath nitrogen washout (MBNW), and how this may be affected by changes in airway volume and resistance.MethodsConsecutive patients (n=21) with severe asthma scheduled for BT, were evaluated at baseline, 6 weeks and 6 months after completion of treatment. Assessments included the Asthma Control Questionnaire (ACQ), medication usage, exacerbation frequency, spirometry, plethysmography and MBNW. Eighteen of these patients underwent detailed CT evaluation for the estimation of airway volume at baseline and then after the left lung had received BT treatment but prior to right lung treatment. Data are mean±STDEV.ResultsPatients responded to BT with an improvement in ACQ from 3.4±0.8 at baseline to 2.0±1.1 at 6 months (p<0.001). Steroid requiring exacerbations fell from 3.1±2.9 in the 6 months prior to BT to 1.4±1.7 following BT (p<0.001). Significant reductions in maintenance oral steroid dosing and short acting beta agonist use were observed.Airway volume measured by CT scanning significantly increased after treatment. The FEV1 improved from 1.34±0.65 l to 1.52±0.76 l (p=0.024). The Residual Volume fell from 2.87±0.89 l to 2.71±0.93 l (p=0.008) and Total Airway Resistance (Raw) from 10.58±6.56 to 7.64±3.74 cmH2O.s.l-1 (p=0.020). The Lung Clearance Index (LCI) was 187±63% predicted at baseline and improved after treatment from 12.7±3.3 to 11.8±2.4 (p=0.049). The improvement in LCI correlated with the improvement in Raw (r=0.463, p=0.035). Conclusion Clinical benefit after BT is accompanied by improvements in lung physiology, including normalisation of lung homogeneity that seems to be driven by airway dilation and reduced resistance.
Treatment of zinc plating wastewater by combination of electrocoagulation and ultrafiltration process
Authors: Öztel Merve Dönmez , Kuleyin Ayşe , Akbal Feryal 
Published: August 2020

Abstract
In this study treatment of zinc plating wastewater by combination of electrocoagulation (EC) and ultrafiltration (UF) processes was investigated. The effect of operating parameters such as wastewater pH, flow rate and membrane pore size on zinc removal was investigated to optimize the EC-UF process. The results showed that zinc removal was pH dependent and optimum pH was 9.0. The zinc removal over 99% was achieved by the combined EC-UF process with both 50 and 100 kDa membranes at pH 9.0 and current density of 5 mA cm−2. Suspended solids and oil–grease were also removed completely by the combined EC-UF process. The EC-UF process proved to be a promising technology for the treatment and recycling of zinc plating wastewater.
CORRELAÇÃO ENTRE OS ÍNDICES ANTROPOMÉTRICOS E PRESSÃO ARTERIAL DE ADOLESCENTES E ADULTOS JOVENS EM UM MUNICÍPIO DO NORDESTE BRASILEIRO
Authors: Cassiano Maria Helena , Santana Luz Anna Beatriz , Bezerra Mariana Silva , Barbosa Suamy Sales , Silva Hémyllen Taísa Diniz da , Araújo Daline Fernandes de Souza 
Published: August 2020

Introdução: A Hipertensão Arterial Sistêmica tem apresentado alta mortalidade em todo o mundo, associada a fatores de risco cardiovascular como o excesso de peso e a obesidade abdominal. Objetivo: Avaliar os índices antropométricos e pressão arterial em adolescentes e adultos jovens do município de Santa Cruz-RN. Método: Trata-se de um estudo quantitativo, do tipo transversal, realizado com 86 indivíduos com idade média de 19,0 ± 0,97 anos. Foram avaliados o Índice de Massa Corporal (IMC), Relação Cintura Estatura (RCE), Relação Cintura Quadril (RCQ), Índice de Conicidade (IC) e Pressão Arterial (PA). Os dados foram analisados no programa SPSS versão 23.0, apresentados em percentual, média e desvio padrão. O teste T de Student foi aplicado para avaliar a diferença entre as médias, a correlação entre medidas antropométricas e a pressão arterial pela correlação de Pearson. Resultados: A prevalência maior foi do sexo feminino, 81,4%. A obesidade esteve mais presente nos meninos adolescentes do que nas meninas, 33,3 e 13,9% respectivamente, bem como nos adultos jovens 28,6% em homens e 11,8% em mulheres. A RCE se mostrou mais elevada nas meninas e mulheres adultas (0,46 ± 0,07, 0,50 ± 0,08). Em contrapartida, a RCQ e IC se mostraram maiores nos meninos (0,79 ± 0,06; 1,12 ± 0,74) e nos homens adultos (0,82 ± 0,09; 1,15 ± 0,12) respectivamente. Em ambos os grupos houve correlação positiva moderada entre o IMC e a RCE com a PA (p<0,05). Conclusões: Os índices antropométricos apresentaram correlação positiva com a elevação da pressão arterial, destacando-se o IMC e a RCE nos adolescentes e adultos jovens.
Palavras-Chave: Antropometria, Estado Nutricional, Hipertensão Arterial.
Therapeutic Options for Mucopolysaccharidosis II (Hunter Disease)
Authors: Kubaski Francyne , Vairo Filippo , Baldo Guilherme , de Oliveira Poswar Fabiano , Corte Amauri Dalla , Giugliani Roberto 
Published: August 2020

Background:Mucopolysaccharidosis type II (Hunter syndrome, or MPS II) is an X-linked lysosomal disorder caused by the deficiency of iduronate-2-sulfatase, which leads to the accumulation of glycosaminoglycans (GAGs) in a variety of tissues, resulting in a multisystemic disease that can also impair the central nervous system (CNS).Objective:This review focuses on providing the latest information and expert opinion about the therapies available and under development for MPS II.Methods:We have comprehensively revised the latest studies about hematopoietic stem cell transplantation (HSCT), enzyme replacement therapy (ERT - intravenous, intrathecal, intracerebroventricular, and intravenous with fusion proteins), small molecules, gene therapy/genome editing, and supportive management.Results and Discussion:Intravenous ERT is a well-established specific therapy, which ameliorates the somatic features but not the CNS manifestations. Intrathecal or intracerebroventricular ERT and intravenous ERT with fusion proteins, presently under development, seem to be able to reduce the levels of GAGs in the CNS and have the potential of reducing the impact of the neurological burden of the disease. Gene therapy and/or genome editing have shown promising results in preclinical studies, bringing hope for a “one-time therapy” soon. Results with HSCT in MPS II are controversial, and small molecules could potentially address some disease manifestations. In addition to the specific therapeutic options, supportive care plays a major role in the management of these patients.Conclusion:At this time, the treatment of individuals with MPS II is mainly based on intravenous ERT, whereas HSCT can be a potential alternative in specific cases. In the coming years, several new therapy options that target the neurological phenotype of MPS II should be available.
Epigenetic Evaluation of N-(2-hydroxyphenyl)-2-Propylpentanamide, a Valproic Acid Aryl Derivative with Activity Against HeLa Cells
Authors: Luna-Palencia G.R. , Correa-Basurto J. , Trujillo-Ferrara J. , Meraz-Ríos M.A. , Vásquez-Moctezuma I. 
Published: August 2020

Background:
Valproic acid (VPA) is an HDAC inhibitor (HDACI) with an anticancer
activity, but is hepatotoxic. N-(2-hydroxyphenyl)-2-propylpentanamide (o-OH-VPA) is a VPA aryl
derivative designed in silico as a selective inhibitor of HDAC8 with biological properties against
HeLa, rhabdomyosarcoma and breast cancer cell cultures.

Objective:
We studied the epigenetic mechanism of o-OH-VPA as an HDACI and evaluated
whether it was toxic to normal cells.

Methods:
HeLa cells and primary human fibroblasts were used for this study as carcinogenic and
normal cells, respectively. Cell survival was evaluated by MTT assay, whereas viability and doubling
time were determined by the Trypan-blue method. HDAC activity was tested using the colorimetric
HDAC activity assay. The expression of p21 was analyzed by PCR and HDAC8 expression
was also evaluated by real-time PCR. Cell cycle and caspase-3 activity were analyzed by flow cytometry
and caspase-3 colorimetric assay, respectively.

Results:
o-OH-VPA (IC50 = 0.1 mM) was fifty-eight times more effective than VPA (IC50 = 5.8
mM) to reduce HeLa cell survival. Furthermore, o-OH-VPA increased the doubling time of HeLa
cells by 33% with respect to the control. o-OH-VPA acted as HDACI in HeLa cells without affecting
the HDAC8 expression, arresting the cell cycle of HeLa cells in the G0/G1 phase due to the increase
in p21 expression with the inhibition of caspase-3 activity without exhibiting toxicity toward
normal cells.

Conclusion:
Our results revealed that o-OH-VPA is an HDACI with a selective effect against
HeLa cells but without the known toxicity exerted by most pan-HDACIs on normal cells.
PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF ACTINIOPTERIS RADIATA
Authors: SANKAR KANDUKURI GOURI , VENKATESWARLU BENDI SRI 
Published: August 2020

Objective: The medicinal plants have been using to treat ailments since ancient times. The recent advances in science and technology impel humans to evaluate medicinal plants therapeutic efficiency and isolation of bioactive compounds in pure forms before their use in development of new drugs and their derivatives. But even now, abundant medicinal plants unevaluated scientifically. The current study was aimed to explore phytochemical constituents, antioxidant and hepatoprotective activities of Actiniopteris radiata root parts.
Methods: Standard procedures have been used to perform phytochemical analysis. Antioxidant activity was carried using In vitro methods on superoxide, hydroxyl, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. Hepatoprotective activity was studied by paracetamol-induced liver toxicity on WISTAR albino rats. The parameters assessed were Aspartate aminotransferase (SGOT/AST), Alanine aminotransferase (SGPT/ALT), alkaline phosphatase (ALP) and total bilirubin levels.
Results: The tested extracts (hexane, ethyl acetate, and hydro-alcoholic) possess biologically active compounds such as sterols, terpenoids, glycosides, phenolics, alkaloids, flavonoids. The hydro-alcoholic extract has more phenolic contents (24.28±0.3) and flavonoid contents (22.68±0.6). The extracts showed dose dependent activity on tested free radicals and extracts showed more percentage inhibition at 320µg. The hydro-alcoholic extract showed more percentage inhibition i.e. 71.00±2.08 on DPPH free radical, 79.67±1.20 on hydroxyl free radical and 80.33±1.20 on superoxide free radical. As antioxidant activity of hexane and ethyl acetate extracts was less and they also showed less percentage protection on liver toxicity, hydro-alcoholic extract showed more percentage protection on biomedical enzyme levels of liver toxicity at high concentration i.e., 400 mg/kg b.w. The percentage protection on the enhancement of AST (SGOT), ALT (SGPT), ALP, and total bilirubin levels were 82.24%, 82.14%, 84.18%, and 82.85% are significant (P<0.01) as Liv52 shown percentage protection on the enhancement of Aspartate aminotransferase (SGOT), alanine aminotransferase (SGPT), Alkaline phosphatase (ALP) and total bilirubin levels were 93.58%, 92.83%, 94.67% and 93.57%.
Conclusion: The current study was aimed to explore phytochemical constituents, antioxidant and hepatoprotective activities of Actiniopteris radiata root parts extracts. The outcome of the current research results provides scientific evidence of the traditional usage of Actiniopteris radiata.
PHYTOCHEMICAL PROFILES OF THREE TRADITIONAL MEDICINAL PLANTS AND ITS ANTIMICROBIAL ACTIVITIES
Authors: JOSEPH RESHMA MARIA , PRAKASIA PRESSY P. 
Published: August 2020

Objective: The objective of the current study was to compare the phytochemical composition and to evaluate the antibacterial properties of Cissus qudrangularis, Cinnamomum zeylanicum and Trigonella foenum-graecum against the bacterial strains Escherichia coli and Bacillus circulans.
Methods: Qualitative analysis and quantitative estimation of various phytochemical components were done using standard protocols. Antibacterial activity against gram-negative Escherichia coli and gram-positive Bacillus circulans was evaluated using standard protocol of agar well diffusion and disc diffusion assay. The zone of inhibition was calculated.
Results: Preliminary phytochemical analysis showed the presence of alkaloids, saponins and tannins in all three plant extracts. In quantitative estimation, Cinnamomum zeylanicum showed high alkaloid content (22%), Cissus qudrangularis showed high saponin content (6%) Trigonella foenum-graecum showed a high concentration (4.65 mg/g) of tannin. All the three plants showed moderate antimicrobial activity. The water extract of Cinnamomum zeylanicum showed the highest zone of inhibition (13 mm) against Escherichia coli and the water extract of Trigonella foenum-graecum showed the highest zone of inhibition (11 mm) against Bacillus circulans.
Conclusion: The result of this study supports the use of all the selected three medicinal plants as a source of antibacterial substance for the possible treatment of human pathogenic organisms. These plants can be further subjected to isolation of the therapeutic phytochemicals and further pharmacological evaluation.
EFFICACY OF BINARY COMBINATION OF DELTAMETHRIN+MGK-264 ON LEVELS OF BIOCHEMICAL CHANGES IN THE SNAIL LYMNAEA ACUMINATA
Authors: SINGH R. N. , KUMAR PRADEEP , KUMAR NAVNEET , SINGH D. K. 
Published: August 2020

Objective: The objective of the present study is the evaluation of the effect of the sublethal (40% and 60% of 48h LC50) binary combination (1:5 ratios) of molluscicides deltamethrin+MGK-264 on the endogenous levels of protein, amino acid and nucleic acid in different tissues of snail Lymnaea acuminata.
Methods: The snails were treated with 1:5 mixtures of sub-lethal concentration of (40% and 60% of 48h LC50) deltamethrin+MGK-264 on the protein, amino acid and nucleic acid levels in gonadal, nervous and foot tissue of L. acuminata. In order to study the effect of withdrawal from treatment, the snails were first exposed to the above concentrations for 96h, after which they were transferred to freshwater. Water was changed every 24h for the next seven days, after which different biochemical parameters were estimated.
Results: There was a significant change in the levels of protein (sublethal concentration of 60% of 48h LC50 after 96h) gonadal, nervous and foot tissues are 48.1, 12.1 and 14.5%, respectively, amino acid are 273, 234 and 252%, respectively, DNA are 25.1, 38.9 and 42.1%, respectively and RNA are 12.2, 30.7 and 30.5%, respectively. These changes were time and concentration-dependent. In the withdrawal experiment, the snails were treated for 96h to transfer in freshwater for 7 d, which caused significant recovery in all the biochemical parameters.
Conclusion: The present study concluded that the high molluscicidal activity of deltamethrin+MGK-264 simultaneous decrease in the levels of proteins, DNA, RNA and increase in the level of amino acids.
Hermanas olvidadas de “genios” mexicanos: María del Refugio Ponce y María Teresa Lara
Authors: Chibici-Revneanu Claudia 
Published: August 2020

Objetivo: recuperar datos sobre las vidas y obras de María Teresa Lara y María del Refugio Ponce –hermanas de los reconocidos “genios” musicales Agustín Lara y Manuel M. Ponce– , dos creadoras prácticamente ignoradas por el canon musical eurocéntrico y por discusiones académicas sobre compositoras mexicanas. Aplicar teorías acerca de la noción de genio como cómplice ideológico de estructuras patriarcales en el campo musical para investigar de qué forma el culto a sus hermanos podría haber provocado la casi desaparición de estas creadoras.Diseño metodológico: el artículo se basa en una investigación interdisciplinaria que reúne musicología, estudios de género y creatividad. Se realizó también un trabajo de campo en Tlatlauquitepec (Puebla) para rescatar datos sobre María Teresa Lara. En el caso de María del Refugio Ponce, se llevó a cabo un estudio de documentos relevantes y partituras en el Archivo General del Instituto Cultural de Aguascalientes.Resultados: se obtuvieron datos biográficos importantes sobre ambas mujeres, así como indicios de que la ideología del genio y el culto a sus hermanos ha sido parcialmente beneficioso, pero sobre todo una limitante en el desarrollo artístico de las creadoras.Limitaciones de la investigación: la historia de María Teresa Lara todavía se encuentra envuelta en contradicciones y aún hace falta un trabajo muy detallado para determinar el catálogo completo de la obra de María del Refugio Ponce.Hallazgos: el artículo revela la prolífica labor musical de María del Refugio Ponce y la probabilidad de una compleja colaboración artística entre los hermanos Lara que anteriormente ha sido soslayada.
Kesesuaian Antara Ketersediaan Antibiotik dan Formularium Nasional Pada Era JKN di Faskes Tingkat Pertama Kota Manado Tahun 2014 - 2017
Authors: Prayitno Lukman , Yuniar Yuyun , Rosita Tita 
Published: August 2020

Abstract
Benefits of Fornas are guidance in determining drug use, increasing rational drug use, quality control and treatment costs and optimizing services. The use of antibiotics is regulated according to the category of health service facilities. Therefore it is necessary to evaluate the suitability between the availability of antibiotic drugs and national formulary. The study design was cross sectional. This type of research is quantitative followed by qualitative data collection. Quantitative data in the form of secondary data on the availability of antibiotics for the period 2014-2017 at the North Sulawesi Provincial Health Office, Manado City Health Office, and 2 Health Centers in Manado City. The average percentage of suitability of antibiotics with the national formulary from 2014 to 2017 at level 1 health facilities was 61.77% in the Provincial Health Office, 61.59% in the City Health Office, 50.14% in the Puskesmas Bahu, 56.85% in the Tikala Health Center. The low percentage of conformity with fornas in the Provincial Health Office, Manado City Health Office and 2 Health Centers was dominated by the large number of antibiotic use which can only be used for level 2 and 3 health facilities. There needs to be escorting the process of socialization, implementation and supervision. It is necessary to consider forming a particular system, for example reward and punishment for doctors who write prescriptions.
Keywords: JKN, Antibiotics, National Formulary, FKTP
Abstrak
Manfaat Fornas yaitu acuan penetapan penggunaan obat dalam JKN, meningkatkan penggunaan obat rasional, kendali mutu dan biaya pengobatan dan mengoptimalkan pelayanan. Penggunaan antibiotik diatur berdasarkan kategori fasilitas pelayanan kesehatan. Oleh karena itu perlu dilakukan evaluasi kesesuaian antara ketersediaan obat antibiotik dan formularium nasional. Desain penelitian adalah cross sectional. Jenis penelitian ini adalah kuantitatif yang diikuti oleh pengumpulan data kualitatif. Data kuantitatif berupa data sekunder ketersediaan antibiotik selama periode 2014 – 2017 di Dinas Kesehatan Provinsi Sulut, Dinkes Kota Manado, dan 2 Puskesmas di Kota Manado. Rata-rata persentase kesesuaian antibiotik dengan formularium nasional dari tahun 2014 – 2017 pada faskes tingkat 1 adalah 61,77% di Dinkes Provinsi, 61,59% di Dinkes Kota, 50,14% di Puskesmas Bahu, 56,85% di Puskesmas Tikala. Rendahnya persentase kesesuaian dengan fornas di Dinkes Provinsi, Dinkes Kota Manado dan 2 Puskesmas lebih didominasi karena banyaknya penggunaan antibiotik yang hanya boleh digunakan untuk faskes tingkat 2 dan 3. Perlu ada pengawalan terhadap proses sosialisasi, implementasi dan pengawasan. Perlu dipertimbangkan untuk membentuk sistem tertentu, misalnya reward and punishment terhadap dokter yang menulis resep.
Kata kunci: JKN, Antibiotik, Formularium Nasional, FKTP
Merokok dan Karies Gigi di Indonesia: Analisis Lanjut Riskesdas 2013
Authors: Tjahja Indirawati 
Published: August 2020

Abstrak
Merokok merupakan hal yang biasa ditemui di dunia khususnya di Indonesia. Kebiasaan merokok ini banyak dijumpai pada orang yang berpendidikan tinggi, rendah, berbagai umur, muda maupun umur lanjut, laki-laki, perempuan, bekerja, tidak bekerja, miskin maupun tidak miskin, meskipun merokok diketahui mengganggu kesehatan. Karies gigi merupakan masalah yang utama pada kesehatan gigi–mulut. Tujuan penelitian untuk mengetahui apakah terdapat hubungan antara umur, jenis kelamin, pendidikan, pekerjaan, status ekonomi, dan merokok dengan karies gigi. Metode dengan menggunakan desain penelitian potong lintang (Cross Sectional). Sampel penelitian adalah semua anggota rumah tangga yang berusia 15 tahun ke atas. Sampel karies gigi berjumlah 173.828 orang. Tidak terdapat hubungan antara merokok dengan karies gigi dengan nilai p:0,664. Namun terdapat hubungan yang bermakna antara variabel umur, jenis kelamin, pendidikan, pekerjaan, sosial ekonomi dengan karies gigi dengan nilai p: 0,000. Bagi perokok maupun bukan perokok yang mempunyai masalah dengan karies gigi sebaiknya ke dokter gigi untuk dilakukan perawatan gigi berupa penambalan gigi atau perawatan saluran akar gigi.
Kata kunci: merokok, karies gigi, riskesdas Abstract
Smoking is a common behaviur in the world, especially in Indonesia. We meet this smoking habit in people who are highly or low educated, in various ages, include men, women although smoking is known to be dangerous interfere with they are health. Dental caries is a major problem in oral health. Objective whether there is a relationship between smoking and dental caries, is there a relationship between age, sex, education, occupation and socio-economic. The purpose of this study is there a relationship between smoking behavior and dental caries. Method by using the a Cross Sectional research design. The sample of study was all household members aged 15 years and over. The dental caries samples numbered 173,828 people. There was no relationship between smoking behavior and dental caries, with p value of 0.664. But there is a meaningful relationship between the variables of age, gender, socio-economic, education, occupation, with dental caries with p value: 0,000. For smokers and non smokers who have problems with dental caries should go to the dentist and having the dental care in the form of dental fillings or root canal treatment.
Keywords: smoking, dental caries, riskesdas