bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-SARS and anti-HCV drugs repurposing against the Papain-like protease of the newly emerged coronavirus (2019-nCoV)
Authors: Elfiky Abdo , Ibrahim Noha S 
Published: February 2020

Abstract
A new mysterious coronavirus outbreak started last month in China. The World Health Organization (WHO) termed the new virus strain 2019-nCoV to be the seventh reported human coronaviruses (HCoV). A seafood market in Wuhan city, central China was the starting point of the emergence with unknown animal causes the first animal to human infection. Until today 904 confirmed deaths and more than 40000 cases confirmed in China and 28 countries. There is a massive fear of the human to human transmission of 2019-nCoV that reported last week by the Chinese government. The most famous two strains of HCoV are the Severe Acute Respiratory Syndrome coronavirus (SARS CoV) and the Middle East Respiratory Syndrome coronavirus (MERS CoV). The former had emerged in China in 2002 while the latter emerged in the Middle East region in 2012 and south Korea in 2015. In this study, the newly emerged 2019-nCoV papain-like protease (PLpro) is targeted by anti-SARS PLpro drugs and the anti-Hepatitis C Virus (HCV) Non-structural protein 3 (NS3) serine protease drugs. Sequence analysis, modeling, and docking are used to get a valid model for 2019-nCoV PLpro. The results suggest the effectiveness of the anti-SARS drugs (GRL-0667, GRL-0617, and Mycophenolic acid) and the anti-HCV drugs (Grazoprevir, Telaprevir, and Boceprevir) as potent inhibitors against the newly emerged coronavirus.
Basic Criteria, Models, and Indicators of Intersectoral Collaboration in Health Promotion: A Scoping Study
Authors: Author) noora rafiee(Former Corresponding , Damari Behzad , Hajebi Ahmad , Goudarzi reza , Author) Mohammad Reza Amir Esmaili(New Corresponding , Haghshenas Abbas 
Published: February 2020

Abstract
Background: The healthcare sector has a small share in community health, and other sectors are actively involved in this domain. Therefore, only by a collective and collaborative action can health promotion interventions be properly implemented. In order to have intersectoral collaboration in the domain of healthcare, basic criteria are required as an operational guide for the successful implementation of such collaborations. A specific framework is also required so that the programs can be properly implemented accordingly. After the implementation, the success or failure of the collaborative action will be determined by assessing the effectiveness of the actions taken using an appropriate assessment system (e.g., indicators). In this study, the basic criteria, models, and indicators of intersectoral collaboration in health promotion were investigated to facilitate the implementation of collaboration. Methods: This scoping review was conducted using datasets of Embase, Web of Science, Scopus, and PubMed, and search engines of Google, Google Scholar, and ProQuest. Original articles, review articles, and reports were included in this study. In terms of subject, articles which referred to the basic criteria, models, or indicators of intersectoral collaboration in health promotion were included. Results: A total of 52 studies were included in the final analysis, and 32 codes in three categories (micro, meso, and macro) were obtained. Micro-level intersectoral collaboration criteria had the highest frequency. Among the models used in the reviewed studies, social network analysis (SNA), Diagnosis of Sustainable Collaboration (DISC), Bergen, and logic models had the highest frequency in health promotion. Among the indicators studied, the number of participants and the level of collaboration as well as its sustainability were the most frequent indicators. Conclusion: The findings identified the most important and widely used criteria, models, and indicators of intersectoral collaboration in health promotion which can be useful for decision-makers and planners in the domain of health promotion, in designing, implementing, and evaluating collaborative programs.
Hepatitis B Virus Can Be Sensed by STING-dependent DNA Sensors but Suppresses the DNA Sensing Pathway in Humans with Acute and Chronic Hepatitis B Virus Infection
Authors: Chen Hongtao , He Guirong , Chen Yue , Zhang Xiaoyong 
Published: February 2020

Abstract
Background : With the discovery of stimulator of interferon genes (STING) in 2008, many intracellular molecules have been identified as STING-dependent DNA sensors in recent years. Studies show the DNA sensor-STING-IFN-β pathway plays an important role in defense against viral infections. However, the recognition of hepatitis B virus (HBV) by DNA sensors is still largely unclear. In this study, we aimed to investigate the role of STING-dependent DNA sensors in HBV infection.
Methods: The expression of these DNA sensors in PBMCs from patients with acute and chronic HBV infection was analyzed by quantitative real-time PCR. The cellular source of the upregulated DNA sensor and the response of the corresponding sensing pathway in PBMCs from HBV-infected patients were also investigated.
Results: The expression of interferon gamma inducible protein 16 (IFI16), DEAD-box polypeptide 41 (DDX41), meiotic recombination 11 homolog A (MRE11), and STING were upregulated in chronic HBV-infected patients, whereas only IFI16 expression increased in acute HBV-infected patients. However, no IFN-β induction was observed in PBMCs in either acute or chronic HBV-infected patients. We next found IFI16 was mainly expressed in monocytes from acute and chronic hepatitis B patients. By stimulation of monocytes with VACV ds 70mer, an agonist ligand for IFI16, we confirmed the suppressed response of the IFI16-STING pathway in chronic HBV infection.
Conclusions: Our results suggest that HBV can be sensed by DNA sensors in PBMCs in both acute and chronic HBV-infected patients and meanwhile actively counteract the DNA sensing pathway in PBMCs, which may facilitate the persistence of HBV infection.
WITHDRAWN: A Double Blind Randomized Clinical Trail: Comparing Lactobacillus plantarum 299v with Placebo in treating irritable bowel syndrome patients
Authors: haq Moeen-ul- , Ullah Fazl , Hassan Muhammad Kamran , Babar Ahmad Nawaz , Ullah Anwar 
Published: February 2020

Abstract
Irritable bowel syndrome (IBS) is a gastrointestinal disease of intestinal mobility. IBS present with variable clinical symptoms making the treatment difficult. IBS is quiet prevalent around the globe with different frequency. Differences in frequency and gender is due to diet habit. It is less frequent where diary product and vegetable are frequently consumed as compared to those who consumed meat. Lactobacillus plantarum 299v (L. plantarum 299v) is the most widely studied strain in the IBS patients. It is resistant to the actions of intestinal acids and bile, colonizes the human colonic mucosa and is non-pathogenic in nature. The efficacy of Lactobacillus plantarum 299v L is different in different study. The present study was designed to find the efficacy of Lactobacillus plantarum 299v in comparison to placebo in randomized control trial. MethodOne hundred and ninety patients were assessed for eligibility 46 among them were excluded from the study and twenty four declined to participate in the study. One hundred and twenty patient of IBS was grouped in two different groups. The one was treated with Lactobacillus plantarum 299v and the other was treated with placebo. Symptoms of IBS, like abdominal pain, bloating and complete rectal emptying was noted and interpreted among the groups. Results There was no statistically significant difference in relieving abdominal pain, bloating, rectal emptying in Lactobacillus plantarum 299v treated group and placebo treated group. Conclusion This randomized control trail of Lactobacillus plantarum 299v fail to show signicant efficacy in IBS treatment as compared to placebo.
Strategi Kemitraan Penyelenggaraan Parenting Bagi Orang Tua di Lembaga PAUD Kecamatan Bumiayu Kabupaten Brebes
Authors: Wiyani Novan Ardy 
Published: February 2020

<p>The purpose of this community service is to provide knowledge both theoretically and practically related to child education methodology (pedagogy). Community service activities using andragogy approach and counseling methods. There are four results achieved from this activity. First, participants gain knowledge about the position of parents in educating children based on the characteristics of children and gain knowledge about positive relationships between fathers and mothers in the process of educating children in the family environment. Second, parents already have strong reasons that make them have to prioritize children's education from an early age. Third, parents have the assumption that habituation activities undertaken to shape the character of children in PAUD institutions must be accompanied by habituation activities carried out in the family environment. Fourth, parents are able to identify the level of addiction to the use of gadgets on themselves and get knowledge about 10 ways to discipline the use of gadgets in children.</p><p> </p><p>Tujuan dari pengabdian masyarakat ini adalah untuk memberikan pengetahuan baik secara teoritis maupun praktis terkait dengan metodologi pendidikan anak (pedagogi). Kegiatan layanan masyarakat menggunakan pendekatan andragogi dan metode konseling. Ada empat hasil yang dicapai dari kegiatan ini. Pertama, peserta mendapatkan pengetahuan tentang posisi orang tua dalam mendidik anak berdasarkan karakteristik anak dan mendapatkan pengetahuan tentang hubungan positif antara ayah dan ibu dalam proses mendidik anak di lingkungan keluarga. Kedua, orang tua sudah memiliki alasan kuat yang membuat mereka harus memprioritaskan pendidikan anak sejak usia dini. Ketiga, orang tua memiliki asumsi bahwa kegiatan pembiasaan yang dilakukan untuk membentuk karakter anak-anak di lembaga PAUD harus disertai dengan kegiatan pembiasaan yang dilakukan di lingkungan keluarga. Keempat, orang tua dapat mengidentifikasi tingkat kecanduan penggunaan gadget pada diri mereka sendiri dan mendapatkan pengetahuan tentang 10 cara untuk mendisiplinkan penggunaan gadget pada anak-anak.</p>
Penguatan Desain Dakwah Masjid di Era Milenilal melalui Android
Authors: Jasuri Jasuri , Khalif M. Ardhi , Anwar Edi Daenuri 
Published: February 2020

<p>Dedication work with the title "Design Da'wah Mosque in Millennial Era through Android" uses a community based research (CBR) model, namely by providing assistance in making mosque website design, website management and website content management in eight mosques in Central Java. The meeting point of da'wah that is carried out in the mosque either through recitation, or other forms of activity can be well documented via the internet and can be accessed by Muslims at large. The need for revitalization of the Da'wah Mosque Strategy, revitalization in question is the Da'wah Mosque Strategy is carried out massively, recorded, and uploaded on the mosque website. By uploading it on the website of the mosque, all people in need can download study materials and can see / listen at any time. Of the eight mosques, only two mosques have websites, the Baiturahman Mosque in Semarang and M asjid Agung in Central Java. This assistance resulted in a pretty good design. The website that has been designed by the mosque admin is good with menus and wicets relating to the activities of the mosque preaching. The mosque website is filtered in one "dakwah online" application. Users can enjoy the da'wah of the mosques from the eight mosques in real time effectively and efficiently.</p><p> </p><p>Pengabdian dengan topik “Desain Dakwah Masjid di Era Milenial melalui Android” ini menggunakan model <em>community based research</em> (CBR), yaitu dengan melakukan pendampingan pembuatan desain website masjid, pengelolaan website dan manajemen konten website pada delapan masjid di Jawa Tengah. Titik temu dakwah yang dilaksanakan di masjid baik melalui pengajian, maupun bentuk-bentuk kegiatan yang lain bisa didokumentasi dengan baik melalui internet dan dapat diakses oleh umat islam secara luas. Perlu adanya revitalisasi Strategi dakwah masjid, revitalisasi yang dimaksud adalah Strategi dakwah masjid dilakukan secara masif, terdata, dan diunggah dalam website masjid. Dengan diunggah dalam website masjid maka semua masyarakat yang membutuhkan bisa mendownload bahan-bahan pengajian dan bisa melihat/mendengarkan setiap saat. Dari delapan masjid dimaksud hanya dua masjid yang sudah memiliki website yaitu Masjid Baiturahman Semarang dan Masjid Agung Jawa Tengah. Pendampingan ini menghasilkan desain yang cukup baik. Website yang telah di desain oleh admin masjid sudah baik dengan menu dan <em>wicget</em> yang berkaitan dengan kegiatan-kegiatan dakwah masjid. Website masjid tersebut ter<em>sharing</em> dalam satu aplikasi “dakwah online”. <em>User</em> bisa memenikmati dakwah masjid dari delapan masjid tersebut secara realtime dengan efektif dan efisien.</p>
Plant-wide modelling in wastewater treatment: showcasing experiences using the Biological Nutrient Removal Model
Authors: Seco A. , Ruano M. V. , Ruiz-Martinez A. , Robles A. , Barat R. , Serralta J. , Ferrer J. 
Published: February 2020

Abstract
Plant-wide modelling can be considered an appropriate approach to represent the current complexity in water resource recovery facilities, reproducing all known phenomena in the different process units. Nonetheless, novel processes and new treatment schemes are still being developed and need to be fully incorporated in these models. This work presents a short chronological overview of some of the most relevant plant-wide models for wastewater treatment, as well as the authors' experience in plant-wide modelling using the general model BNRM (Biological Nutrient Removal Model), illustrating the key role of general models (also known as supermodels) in the field of wastewater treatment, both for engineering and research.
WITMSG: Large-scale Prediction of Human Intronic m6A RNA Methylation Sites from Sequence and Genomic Features
Authors: Liu Lian , Lei Xiujuan , Meng Jia , Wei Zhen 
Published: February 2020

Introduction:
N6-methyladenosine (m6A) is one of the most widely studied epigenetic modifications.
It plays important roles in various biological processes, such as splicing, RNA localization
and degradation, many of which are related to the functions of introns. Although a number of computational
approaches have been proposed to predict the m6A sites in different species, none of them
were optimized for intronic m6A sites. As existing experimental data overwhelmingly relied on polyA
selection in sample preparation and the intronic RNAs are usually underrepresented in the captured
RNA library, the accuracy of general m6A sites prediction approaches is limited for intronic m6A sites
prediction task.

Methodology:
A computational framework, WITMSG, dedicated to the large-scale prediction of intronic
m6A RNA methylation sites in humans has been proposed here for the first time. Based on the
random forest algorithm and using only known intronic m6A sites as the training data, WITMSG takes
advantage of both conventional sequence features and a variety of genomic characteristics for improved
prediction performance of intron-specific m6A sites.

Results and Conclusion:
It has been observed that WITMSG outperformed competing approaches
(trained with all the m6A sites or intronic m6A sites only) in 10-fold cross-validation (AUC: 0.940)
and when tested on independent datasets (AUC: 0.946). WITMSG was also applied intronome-wide in
humans to predict all possible intronic m6A sites, and the prediction results are freely accessible at
http://rnamd.com/intron/.
Cholesterol-Conjugate as a New Strategy to Improve the Cytotoxic Effect of 5-Fluorouracil on Liver Cancer: Impact of Liposomal Composition
Authors: Alanazi Saleh Ayed , Harisa Gamaleldin Ibrahim , Badran Mohammad M. , Haq Nazrul , Radwan Awwad Abdoh , Kumar Ashok , Shakeel Faiyaz , Alanazi Fars Kaed 
Published: February 2020

Purpose:
Hepatocellular carcinoma (HCC) is a common liver malignancy, which has a low
survival rate of all cancers. 5-fluorouracil (5-FU) is clinically recognized to treat HCC. However, the
success of this therapy is highly limited due to rapid clearance and non- selective distribution. Cholesterol-
conjugate (5-FUC) loaded liposomes proposed to facilitate the transport of 5-FUC into tumor cells
via Low-Density Lipoprotein receptor (LDL receptor) that overexpressed in HCC. Thus, the aim of this
study was to use 5-FUC loaded liposome as a promising strategy to combat HCC and improve the response
of HCC to chemotherapy.

Methods:
5-FUC and 5-FU loaded liposomes were optimized based on Cholesterol (CHO) ratio and
type of phospholipid to achieve a potential effect on HCC. Liposomes were prepared by the thin-film
hydration method, and evaluated in terms of particle size, polydispersity, zeta potential, Entrapment
Efficiency (EE), morphology, drug release and cytotoxicity.

Results:
The obtained liposomes had a suitable nano-range particle size with negative zeta potential,
and acceptable EE%. In vitro drug release of 5-FUC loaded liposomes showed a lower cumulative release
over 24 h as compared to 5-FU loaded liposomes. 5-FUC loaded liposomes exhibited a higher in
vitro cytotoxic effect as compared to the free drug and 5-FU loaded liposomes against HepG2 cell lines
after 48 h via MTT assay.

Conclusion:
These results concluded that 5-FUC loaded liposomes could be used as an alternative tactic
to increase the therapeutic index of 5-FU and pave the way for potential clinical applications.
The Impact of Sarcopenic Obesity on Health-Related Quality of Life of Treatment-Seeking Patients with Obesity
Authors: Itani Leila , Kreidieh Dima , El Masri Dana , Tannir Hana , El Ghoch Marwan 
Published: February 2020

Background:There is a paucity of studies on health-related quality of life (HRQoL) and sarcopenic obesity (SO).Objective:This study aimed to assess the potential association between SO and impaired HRQoL.Methods:The ORWELL 97 questionnaire was used to assess HRQoL and body composition was measured using a bioimpedance analyser (Tanita BC-418) in 130 patients with obesity, referred to the Nutritional and Weight Management outpatient clinic of Beirut Arab University in Lebanon. Participants were then categorized on the basis of the absence or presence of SO.Results:Sixty-four of the 130 participants met the criteria for SO (49.2%) and displayed significantly higher total ORWELL 97 scores than those in the group without SO (64.00 vs. 41.00, p=0.001), indicative of poorer HRQoL. Linear regression analysis showed that SO was associated with an increase in ORWELL 97 scores by nearly 24 units (β=24.35, 95% CI=11.45-37.26; p<0.0001). Moreover, the logistic regression analysis showed that SO increased the odds of clinically significant impairment of HRQoL (ORWELL 97 score ≥74.25) by nearly seven-fold (OR=7.37, 95% CI=1.92-28.39; p=0.004).Conclusion:Our findings show that the presence of SO was associated with increased impairment of HRQoL that reaches clinical significance when compared to obesity only. Future studies are needed to clarify whether this may influence clinical outcomes. If this is shown to be the case, weight management programs should incorporate additional strategies to improve HRQoL in individuals with SO.
Electrochemical Biosensor for the Detection of Amygdalin in Apple Seeds with a Hybrid of f-MWCNTs/CoFe2O4 Nanocomposite
Authors: Inamuddin  , Kanchi Suvardhan , Kashmery Heba A. 
Published: February 2020

Background:
Amygdalin is a natural compound known for curing cancer. It is seen in
several plants including in bitter almonds, apricots, peaches, apples, and plum seeds (kernels).
Amygdalin is a toxic molecule containing a nitrile group, due to which toxic cyanide anion releases
by the action of a β-glucosidase. The consumption of amygdalin may lead to cyanide poisoning in
the human body. Therefore, for the first time, this work is aimed at developing a novel electrochemical
biosensor for the detection of Amygdalin (AMG) in apple seed samples.

Methods:
The proposed electrochemical biosensor was fabricated by immobilizing cytochrome c
(Cyt c) on a Glassy Carbon Electrode (GCE) with nanocomposite of cobalt ferrite nanoparticles
(CoFe2O4 NPs) and functionalised multiwalled carbon nanotubes (f-MWCNTs). The characterization
of the synthesized nanocomposite was performed with FTIR, TEM, TGA/DSC, and XRD techniques.
Moreover, various experimental parameters such as the effect of pH, deposition time, sweep rate, potential,
and enzyme incubation time and interference were also studied.

Results:
The fabricated biosensor enhanced the peak current by 10-folds compared to unmodified
GCE. Under optimized experimental conditions, the biosensor exhibited linear response from 2 to 20
μM, with a linear regression equation Ipa (μA) = 8.4989 c + 6.6307 (R² = 0.9927). The LOD’s and
LOQ’s were found to be 0.0112 μM and 0.2213 μM, respectively.

Conclusion:
The designed biosensor was successfully applied for the analysis of AMG content in the
apple seed samples. The outcomes of this study identify the efficient electrocatalytic activity of the
fabricated nanocomposite as significant electronic factors as major contributors to the electron transfer
mechanism, with promising scope for the design of biosensor to sense toxic molecules.
Functional and Structural Characterization of a Novel Isoamylase from Ostreococcus tauri and Role of the N-Terminal Domain
Authors: Hedín Nicolas , Barchiesi Julieta , Gomez-Casati Diego F. , Busi María V. 
Published: February 2020

Background:
The debranching starch enzymes, isoamylase 1 and 2 are well-conserved enzymes present in almost all the photosynthetic organisms. These enzymes are involved in the crystallization process of starch and are key components which remove misplaced α-1,6 ramifications on the final molecule.

Aim:
In this work, we performed a functional and structural study of a novel isoamylase from Ostreococcus tauri.

Methods:
We identified conserved amino acid residues possibly involved in catalysis. We also identified a region at the N-terminal end that resembles a Carbohydrate Binding Domain (CBM), which is more related to the family CBM48, but has no spatial conservation of the residues involved in carbohydrate binding.

Results:
The cloning, expression and biochemical characterization of this N-terminal region confirmed that it binds to polysaccharides, showing greater capacity for binding to amylopectin rather than total starch or amylose.

Conclusion:
This module could be a variant of the CBM48 family or it could be classified within a new CBM family.
Lemon Peel Powder: A Natural Catalyst for Multicomponent Synthesis of Coumarin Derivatives
Authors: Jadhav Ganesh D. , Mujawar Taufique Ahmed P. , Tekale Sunil U. , Pawar Rajendra P. , More Yogesh W. 
Published: February 2020

Background:
Lemon peel powder was used as a natural catalyst for the synthesis of biscoumarins
and 3,4-dihydropyrano[c]chromene derivatives. The catalyst is natural, biodegradable, environmentally
benign and thus contributes a valuable addition to the existing sustainable methods for the
synthesis of coumarin derivatives.

Objective:
Development of Green synthesis and use of Natural catalyst.

Methods:
Lemon peel powder was used as a natural, biodegradable, environmentally benign heterogenous
catalyst for the synthesis of coumarin derivatives.

Results:
Natural lemon peel powder was successfully used as a heterogeneous catalyst for the synthesis
of coumarin based heterocyclic compounds.

Conclusion:
In conclusion, an environmentally benign, green and one-pot multicomponent protocol
has been developed for the synthesis of biscoumarins and 3,4-dihydropyrano[c]chromene derivatives
using lemon peel powder as a natural and biodegradable catalyst. Lemon peel being natural, the present
approach is sustainable and will highlight the emerging applications of natural resources for synthetic
organic transformations. This protocol offers several advantages such as high yields, clean reaction
conditions, and no pollution threat to the environment making it a useful and attractive process for the
synthesis of coumarin derivatives.