bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA-107 promotes apoptosis of acute myelocytic leukemia cells by targeting RAD51
Authors: Huang Fengxia , Tang Wei , Lei Yan 
Published: February 2020

IntroductionThis study aimed to investigate the role of microRNA (miRNA) that affects acute myelocytic leukemia (AML) and its potential molecular mechanism by constructing a miRNA–mRNA interaction network using bioinformatics methods.Material and methodsMicroRNA expression data of AML were retrieved from Gene Expression Omnibus (GEO) and analyzed by microarray analysis. Expression levels of miR-107 and RAD51 mRNA were detected by quantitative real time polymerase chain reaction (qRT-PCR). Protein expression of RAD51, pro-apoptotic protein Bax, apoptosis related protein CytC and anti-apoptotic protein Bcl-2 were determined by Western blot. The rate of cell apoptosis was detected by Annexin-V/PI. The predicted targeting relationship between miR-107 and the 3′UTR of RAD51 was first predicted by the online application TargetScan and then verified by dual-luciferase assay.ResultsAcute myelocytic leukemia-associated genes (n = 197) and miRNAs (n = 1701) were retrieved from the database, the interaction network of miRNA-mRNA was constructed and the core position was occupied by RAD51. miR-107 exhibited a regulatory effect on RAD51 in which the mRNA and protein expression of RAD51 were both significantly inhibited by miR-107 mimics in vitro. Additionally, down-regulated expression of miR107 as well as up-regulated expression of RAD51 were detected not only in the plasma of AML patients compared to healthy volunteers, but also in AML cell lines compared to the normal bone marrow stromal cell line. Further study found that increased expression of miR-107 and the consequent down-regulation of RAD51 could aggravate the apoptosis of AML cells in vitro.ConclusionsOur present results showed that the crucial role of RAD51 and miR-107 in the apoptosis of AML cells, i.e., miR-107 promotes the apoptosis of AML cells through down-regulating the expression of RAD51.
MicroRNA-575 targets Derlin 1 to regulate proliferation, migration and invasion of human thyroid cancer cells
Authors: Dong Hong , Wu Yan-Le , Zhang Xin , Li Han-Lin , Zheng Wei-Hong 
Published: February 2020

IntroductionThis study was undertaken to examine the expression of miR-575 in thyroid cancer tissues and to explore its therapeutic potential.Material and methodsExpression analysis was carried out by qRT-PCR. The MTT assay was used for cell viability. DAPI and annexin V/PI assays were used to detect apoptosis. Wound healing and Transwell assays were used for cell migration and invasion respectively. Western blot analysis was used to determine the expression of proteins.ResultsThe results showed significant downregulation of miR-575 in thyroid cancer tissues and cell lines. The role of miR-575 was deciphered by overexpression of miR-575 in MDA-T32 and MDA-T68 thyroid cancer cells. The results showed that overexpression of miR-575 caused significant inhibition of the proliferation of the MDA-T32 and MDA-T68 cells via induction of apoptotic cell death. The expression of Bax was also enhanced while that of Bax was decreased upon miR-575 overexpression in MDA-T32 and MDA-T68 cells. Additionally, miR-575 overexpression inhibited the migration and invasion of the MDA-T32 and MDA-T68 thyroid cancer cells. Bioinformatic approaches and the dual luciferase assay indicated Derlin 1 (DERL1) to be the potential target of miR-575 in thyroid cancer. DERL1 was significantly upregulated in thyroid cancer tissues and cell lines and overexpression of miR-575 caused suppression of DERL1 in MDA-T68 cells. Silencing of DERL1 inhibited the proliferation of the MDA-T68 cells while overexpression of DERL1 could abolish the effects of miR-575 overexpression on the proliferation of MDA-T68 thyroid cancer cells.ConclusionsmiR-575 may be used as a therapeutic target for thyroid cancer treatment.
A network pharmacological study to unveil the mechanisms of xianlinggubao capsule in the treatment of osteoarthritis and osteoporosis
Authors: Zeng Jianwei , Li Cheng , Gu Zuchao 
Published: February 2020

IntroductionXianlinggubao (XLGB) capsule is a traditional Chinese medicine, which is approved by the Chinese State Food and Drug Administration (CFDA) for osteoarthritis (OA) and osteoporosis (OP). However, as a capsule with complex ingredients, the molecular mechanisms supporting the therapeutic effects have not been explored.Material and methodsA network pharmacology-based approach was conducted to explore the complex interactome among the targets of the XLGB active compounds.ResultsThe herbs in the capsule contain 41 compounds with 246 high score targets, which cover four known OA targets (PTGS1, PTGS2, PTGER4 and TNF) and six known OP targets (AR, ESR1, PGR, PTGER2, TNFSF11 and VDR) of FDA-approved drugs or drugs undergoing clinical trials. The protein-protein interaction (PPI) network of the 246 targets had six key modules. Among the six modules, neuroactive ligand-receptor interaction, cAMP signaling pathway and calcium signaling pathway are the key pathways, which are all closely associated with the degeneration of joint cartilage and bone formation and resorption.ConclusionsNeuroactive ligand-receptor interaction, cAMP signaling pathway, and calcium signaling pathway might be the critical pathways upon which the capsule might act. The present study laid down a foundation to understand the molecular mechanisms of the XLGB capsule and also provided fundamental information for better improvement of the drug with the concept “less herbal materials for achieving equal treatment efficacy”.
Percutaneous left atrial appendage closure for stroke prevention in patients with chronic renal disease
Authors: Lind Alexander , Azizy Obayda , Lortz Julia , Janosi Alexander , Rassaf Tienush , Rammos Christos 
Published: February 2020

IntroductionAtrial fibrillation (AF) is the most common cardiac arrhythmia in the general population. Atrial fibrillation is associated with an increased risk of thromboembolic events, particularly stroke. Chronic kidney disease (CKD) is associated with a higher prevalence of AF and is an independent risk factor of increased mortality and stroke in AF patients. Left atrial appendage closure (LAAC) for stroke prevention plays an important role in the treatment of patients with AF and increased bleeding risk. The impact of CKD on outcomes after LAAC has not been deeply investigated. We assessed whether percutaneous LAAC is safe and feasible in CKD patients.Material and methodsNinety-seven patients (mean age: 73.9 ±8.5 years) with AF and contraindications for oral anticoagulation (OAC) or complications under OAC underwent LAAC with the Amplatzer Cardiac Plug and the Amplatzer Amulet Occluder in an open-label observational single-center study. We classified patients as having normal to mild (KDOQI stage I–II) or moderate to severe (KDOQI stage III–V) CKD.ResultsPatients with moderate to severe CKD (n = 49) had increased CHA2DS2-VASc and HAS-BLED scores and were at higher thromboembolic and bleeding risk (CHA2DS2-VASc: 4.08 ±0.79, HAS-BLED: 4.76 ±0.69) than patients with mild to moderate CKD (n = 48, CHA2DS2-VASc: 3.69 ±1.1, HAS-BLED: 4.06 ±0.66; p < 0.001 for both). In both groups, procedural and occlusion success was similar (97.9% vs. 95.9%; p = 0.479). Follow-up of 6 months and from 12 to 36 months revealed effective stroke prevention and no bleeding complication in both groups.ConclusionsIn spite of a higher thromboembolic and bleeding risk in patients with severe CKD, LAAC is a safe and feasible option for stroke prevention.
TLR4 knockdown by miRNA-140-5p improves tendinopathy: an in vitro study
Authors: Jun Tu , Ruipeng Guo , Bin Xu 
Published: February 2020

IntroductionThe purpose of this study was to determine whether TLR4 knockdown induced by miRNA-140-5p improves tendinopathy in an in vitro experiment.Material and methodsExtraction of tendon-derived stem cells (TDSCs) from SD rats was performed using TGF-1 to develop a tendinopathy cell model. In the first step, we knocked down TLR4 by si-TLR4 to investigate TLR4 in tendinopathy development, and the next we used miRNA-140-5p to investigate miRNA-140-5p in tendinopathy development. The inflammatory factors and Hyp concentration were evaluated by ELISA assay; the cell viability was measured by MTT assay; the cell apoptosis was evaluated by TUNEL and/or flow cytometry. The relative mRNA was measured by RT-qPCR assay; the relative proteins expression was evaluated by cellular immunofluorescence and/or WB assay. The correlation between miRNA-140-5p and TLR4 was analyzed by Luciferase reporter assay.ResultsWith miRNA-140-5p overexpression or TLR4 knockdown, the cell viability was significantly increased with cell apoptosis depressing compared with the Model group (p < 0.05, respectively). Meanwhile, the inflammatory factors TNF-α, IL-1β and IL-6 and Hyp concentration were significantly improved (p < 0.05, respectively), whereas the TLR4, MyD88 and NF-κB(p65) protein expression levels were significantly depressed with TLR4 knockdown by si-TLR4 or miRNA-140-5p which target TLR4.ConclusionsThe present results showed that TLR4 knockdown induced by miRNA-140-5p or si-TLR4 improved tendinopathy in an in vitro cell experiment.
Stilte dirigeren. Michael Maierhofs gebruik van gedirigeerde en gemeten stiltes / Conducting Silence. Michael Maierhof’s use of conducted and measured silences
Authors: Moore Thomas R. 
Published: February 2020

Abstract
Stiltes zijn een essentieel onderdeel van muziek. Wanneer stilte nauwkeurig wordt gemeten, zoals in Michael Maierhofs Zonen 6 voor gitaarorkest, kan deze een hele compositie structureren en de luisteraar helpen om een stuk zin te geven. Het artistieke gebruik ervan heeft ook een dramatische invloed op de rol en de uitvoeringspraktijk van de dirigent. Precies 75% van Zonen 6 voor gitaarorkest van Michael Maierhof bestaat uit gecomponeerde en uitgemeten soundscapes, uitgevoerd door zeventien gitaristen. De andere 25% van het stuk bestaat uit afgemeten en gedirigeerde stilte. In dit artikel peilt Thomas Moore het artistieke gebruik van deze stiltes en onderzoekt hij de manier waarop zij de soundscapes omlijsten en helpen structureren. Ook het nut van de dirigent(e) tijdens deze stiltes wordt overwogen, net als de manieren waarop de dirigent(e) invloed uitoefent op de uitvoering door de gitaristen, en de perceptie van het stuk door het publiek.
Virtual induction ceremonies: Upholding time-honored tradition
Authors: Hopkins Emily E. , Spadaro Kathleen C. 
Published: February 2020

An induction ceremony is a time-honored tradition among honor societies. Designed to recognize new members and denote transition into the society, induction signifies an important step in formal membership. However, with the growth of online education programs, there is a need for honor societies to adapt from traditional on-ground communities to virtual ones. This adaptation includes conducting an induction ceremony virtually to further create a sense of membership belonging and value. Recognizing the importance of upholding a time-honored tradition, a small private liberal arts university in Southwestern Pennsylvania sought to digitally record their international honor society chapter induction ceremony and share it with their distance inductees via their chapter website. Use of the virtual induction began prior to becoming an international chapter and has continued annually for the past 10 years. Therefore, this experience exchange shares strategies for planning and conducting a successful virtual induction ceremony, thus emphasizing the importance of membership induction for all students especially those online.
Simulasi difusi dan adsorpsi matriks pada proses <i>enhanced coalbed methane</i>
Authors: Nurisman Ade , Dewi Retno Gumilang , Siagian Ucok W.R. 
Published: February 2020

Diffusion and matrix adsorption simulations in enhanced coalbed methane process. Carbon capture and storage (CCS) can be considered as one of climate change mitigation efforts, through capturing and injecting of CO2 in underground formations for reducing CO2 emissions. CO2 injection in coalbed methane (CBM) reservoir has potentially attracted for reducing CO2 emissions and enhancing coalbed methane (ECBM) recovery. Diffusion and sorption are phenomenon of gas in the matrix on CO2 injection in CBM reservoir. The objectives of the research are focused on understanding of diffusion and sorption of gas in the coal matrix with mathematical model and estimating of CO2 storage in coalbed and CH4 recovery. In this research, mathematical model is developed to describe the mechanism in the matrix on ECBM process. Mathematical model, which have been valid, is simulated in various variables, i.e. macroprosity (0.001, 0.005, and 0,01), pressure (1, 3, and 6 MPa), temperature (305, 423, and 573 K), and initial fraction of CO2 (0.05, 0.1, 0.3, and 0.5). The results of this research show that preferential sequestration of CO2 and preferential recovery of CH4 in the surface of micropore on macroporosity 0.001, pressure 1 MPa, temperature 305 K, and inital fraction CO2 0,5 conditions are 0.9936 and 0.0064.Keywords: carbon capture and storage (CCS), coalbed methane (CBM), ECBM, diffusion, adsorption Abstrak Carbon capture and storage (CCS) dapat dipertimbangkan sebagai salah satu upaya mitigasi perubahan iklim, yaitu dengan menangkap CO2 dan menginjeksikannya ke dalam formasi bawah permukaan. Injeksi CO2 pada lapangan coalbed methane (CBM) berpotensi mengurangi emisi CO2 dan meningkatkan produksi CBM (ECBM). Pada proses injeksi CO2 di lapangan CBM, fenomena yang terjadi di dalam matriks lapisan batubara (coalbed) adalah difusi dan adsorpsi. Penelitian ini bertujuan memahami fenomena difusi dan adsorpsi pada proses injeksi CO2 untuk ECBM melalui model matematika, dan memperkirakan potensi penyimpanan CO2 di dalam lapangan CBM dan potensi recovery CH4. Pada penelitian dilakukan pengembangan model matematika untuk menjelaskan fenomena di dalam matriks pada proses ECBM. Model matematika, yang telah valid, disimulasikan dengan memvariasikan beberapa variabel, yaitu makroporositas (0,001, 0,005, dan 0,01), tekanan (1, 3, dan 6 MPa), suhu (305, 423, dan 573 K), dan fraksi CO2 awal (0,05, 0,1, 0,3, dan 0,5). Hasil penelitian menunjukkan pada makroporositas 0,001, tekanan 1 Pa, suhu 305 K, dan fraksi CO2 awal 0,5, fraksi CO2 yang teradsorpsi pada permukaan mikropori bernilai 0,9936 dan sisa fraksi CH4 yang teradsorpsi pada permukaan mikropori bernilai 0,0064. Kata kunci: carbon capture and storage (CCS), coalbed methane (CBM), ECBM, difusi, adsorpsi
Sintesis hidroksiapatit berukuran nano dengan metode elektrokimia dibantu EDTA
Authors: Nur Adrian , Martasari Desi Liah , Nurwijayanti Desy , Affandi Samsudin , Widjaja Arief , Setyawan Heru 
Published: February 2020

EDTA-assisted synthesis of nanosized hydroxyapatite by electrochemical method.Synthesis of nanosized hydroxyapatite by electrochemical method has been studied. The aim of this work is to study the effects of variuos parameters on hydroxyapatite particles. The electrochemical synthesis was prepared in solution Ca2+/EDTA/PO43 at concentration 0.25/0.25/0.15 M. The solution was electrolized by using constant direct current at certain current density. There were two treatments for particles after electrosynthesized, namely without aging or aged for 3 days at 40oC. For both cases, the particles were filtered and washed by demineralized water to eliminate the impurities and unreacted reactans. Then, the particles were dryed at 40oC for 2 days. The dryed particles were characterized by X-Ray diffraction, surface area analyzer, scanning electron microscopy, Fourier transform infrared spectra and thermogravitimetric and differential thermal analysis. The results showed that nanosized hydroxyapatite were produced by electrochemical method. The produced particles were composed of needle-like or plate-like morphologies with equivalent diameter 19-143 nm. The produced particles diameters were inversely proportional to current density, duration of electroysis and EDTA concentration, and proportional to the distance between two electrodes and temperature. The product calcium phosphates types depended on the final pH of electrolysis process and aging treatment. It would tend to hydroxyapatite if pH>8 and brushite if pH<8.Keywords: hydroxyapatite, nanoparticle, electrochemical, synthesis, characterize AbstrakPenelitian tentang sintesis hidroksiapatit berukuran nano dengan metode elektrokimia telah dilakukan. Penelitian ini bertujuan mempelajari pengaruh kondisi operasi terhadap pembentukan partikel hidroksiapatit.Elektrosintesis dilakukan dengan menggunakan larutan Ca2+/EDTA/PO43- 0,25/0,25/0,15 M. Ada dua perlakuan sebelum penyaringan yaitu langsung disaring dan dibiarkan dulu selama 3 hari pada 40oC (proses penuaan). Endapan yang dihasilkan kemudian disaring dan dicuci dengan air demin untuk menghilangkan pengotor dan sisa reaktan yang tidak bereaksi. Endapan dikeringkan dengan suhu 40oC selama 2 hari untuk selanjutnya dikarakterisasi. Hasil menunjukkan bahwa dengan metode elektrokimia bisa dihasilkan partikel hidroksiapatit berukuran nano. Partikel yang dihasilkan berbentuk jarum atau plat dengan diameter BET 19-143 nm. Diameter partikel yang dihasilkan berbanding terbalik dengan kenaikan rapat arus, waktu elektrolisa dan konsentrasi EDTA. Sebaliknya, diameter partikel berbanding lurus dengan jarak elektrode dan suhu. Selain itu jenis partikel yang dihasilkan sangat bergantung pada pH akhir dari proses elektrolisis maupun perlakuan penuaan, dimana pH>8 cenderung membentuk HA, sedangkan pH<8 cenderung membentuk fasa brushit.Kata kunci:hidroksiapatit, nanopartikel, elektrokimia, sintesis, karakterisasi
Biological Efficacy of Trichoderma spp. and Bacillus spp. in the Management of Plant Diseases
Authors: Daniel Hernández-Castillo Francisco , Castillo-Reyes Francisco , Antonio Tucuch-Pérez Marco , Arredondo-Valdes Roberto 
Published: February 2020

This chapter will cover topics about the microbial antagonists Trichoderma spp. and Bacillus spp. from the perspective of use as potential biological control agents on plant diseases. Results obtained in the laboratory about from their isolation, microbial strain collections for both genera, taxonomic identification, antifungal activity in in vitro tests, obtained evaluation of the antifungal effect of secondary metabolites from microbial antagonists will be shown. Besides, results obtained from bioassays in the greenhouse and field are used as biopesticides in the control of diseases in fruit trees and vegetables and their effects on the promotion of plant growth and increased crop yield.
What is Capgras Syndrome? Diagnosis and Treatment Approach
Authors: Enzel Koc Aslı , Hocaoglu Cicek 
Published: February 2020

Capgras delusion is a complex psychopathological phenomenon that presents in a wide range of psychiatric and neurological disorders with differing patterns dependent on the main etiology. An underlying neurological disease should be suspected where the delusion concerns a spouse or inanimate objects and is associated with visual hallucinations, while a functional disorder is suggested by multiple imposters, strangers, additional delusions, and auditory hallucinations. Misidentifications in Capgras syndrome (CS) are fixed false beliefs and, therefore, represent true delusions. Even if when patients are confronted over and over with the illogical nature of the delusion, they keep their beliefs. Surprisingly, patients may show implicit or explicit awareness of their true situation. Some research suggests that a considerable number of patients with CS have some awareness of the bizarre nature of the misidentification delusions and therefore tend not to report them, especially during initial interviews when they are less likely to be confident with the clinician. Specific questions and interventions may assist clinicians in successfully identifying patients with CS. In a series of interviews with these patients, some focus on identifying CS, rather than a single interview which is likely to increase the detection of the delusional misidentification. The clinician should always be mindful of the risk of aggression and homicide in CS.
Asymmetric Construction of Bispiro‐Cyclopropane‐Pyrazolones via a [2+1] Cyclization Reaction by Dipeptide‐Based Phosphonium Salt Catalysis
Authors: Lu Dongming , Liu Xin , Wu Jia‐Hong , Zhang Song , Tan Jian‐Ping , Yu Xiaojun , Wang Tianli 
Published: February 2020

AbstractWe reported herein an efficient alternative for asymmetric synthesis of structurally complicated chiral bispiro‐cyclopropane‐pyrazolones via dipeptide‐based phosphonium salt catalyzed [2+1] cyclization of 2,3‐dioxopyrrolidines and halogenated pyrazolones. With this catalytic asymmetric protocol, a broad range of bispiro heterocyclic compounds bearing a pyrazolone unit were prepared in high yields with excellent diastereo‐ and enatioselectivities. Of note, this transformation proceeds in a really short time under mild reaction conditions.magnified image
Targeted Engineering of Medicinal Chemistry for Cancer Therapy: Recent Advances and Perspectives
Authors: Chen Weihua , Sun Zhen , Lu Lehui 
Published: February 2020

AbstractSevere side effects and poor therapeutic efficacy are the main drawbacks of current anticancer drugs. These problems can be mitigated by targeting, but the targeting efficacy of current drugs is poor and urgently needs improvement. Taking this into consideration, this Review first summarizes the current targeting strategies for cancer therapy in terms of cancer tissue and organelles. Then, we analyse the systematic targeting of anticancer drugs and conclude that a typical journey for a targeted drug administered by intravenous injection is a CTIO cascade of at least four steps. Furthermore, to ensure high overall targeting efficacy, the properties of a targeting drug needed in each step are further analysed, and some guidelines for structure optimization to obtain effective targeting drugs are offered. Finally, some viewpoints highlighting the crucial problems and potential challenges of future research on targeted cancer therapy are presented. This review could actively promote the development of precision medicine against cancer.
Nomograms to predict the long‐time prognosis in patients with alpha‐fetoprotein negative hepatocellular carcinoma following radical resection
Authors: Huang Jian , Liu Fu‐Chen , Li Li , Zhou Wei‐Ping , Jiang Bei‐Ge , Pan Ze‐Ya 
Published: February 2020

AbstractBackgroundTo develop and validate nomograms that can be used to predict outcomes in individuals suffering alpha‐fetoprotein (AFP) negative hepatocellular carcinoma (HCC) after radical resection.MethodsA total of 509 AFP‐negative HCC patients who received hepatectomy between January 2009 and March 2013 in our center were randomized into training and validation cohorts. Nomograms for both overall and recurrence‐free survival (OS and RFS, respectively) were established based on the predictors in the training cohort. Nomograms performance and discriminative power were assessed with concordance index (C‐index) values and decision curve analyses (DCA). The results were validated in the validation cohort.ResultsAlkaline phosphatase, liver cirrhosis, tumor size, satellite lesions, microvascular invasion, and Edmondson‐Steiner grade were significantly linked to OS and RFS. Sex and tumor number were additional predictors for RFS. The OS nomogram had a C‐index value of 0.742, which was better than that for the AJCC eighth edition (0.632), BCLC system (0.553), and JIS score (0.557) (all P < .001). The RFS nomogram C‐index was 0.669, which was also superior to that of the AJCC eighth (0.608), BCLC stage (0.554), JIS score (0.551), and model of Gan et al (0.636) (P < .05 for all). Calibration curves indicated a good agreement between observed actual outcomes and predicted values. Kaplan‐Meier curves and DCA indicated that nomograms were powerful in discrimination and clinical usefulness. These results were supported by the validation cohort.ConclusionsThese nomograms presented more accurate prognostic prediction in patients with AFP‐negative HCC after hepatectomy.