bluuebunny/crossref_metadata_2025_split huggingface dataset:
New insights into the structural elements of the upper mantle beneath the contiguous United States fromS-to-Pconverted seismic waves
Authors: Kind Rainer , Mooney Walter D , Yuan Xiaohui 
Published: April 2020

SUMMARYThe S-receiver function (SRF) technique is an effective tool to study seismic discontinuities in the upper mantle such as the mid-lithospheric discontinuity (MLD) and the lithosphere–asthenosphere boundary (LAB). This technique uses deconvolution and aligns traces along the maximum of the deconvolved SV signal. Both of these steps lead to acausal signals, which may cause interference with real signals from below the Moho. Here we go back to the origin of the SRF method and process S-to-P converted waves using S-onset times as the reference time and waveform summation without any filter like deconvolution or bandpass. We apply this ‘causal’ SRF (C-SRF) method to data of the USArray and obtain partially different results in comparison with previous studies using the traditional acausal SRF method. The new method does not confirm the existence of an MLD beneath large regions of the cratonic US. The shallow LAB in the western US is, however, confirmed with the new method. The elimination of the MLD signal below much of the cratonic US reveals lower amplitude but highly significant phases that previously had been overwhelmed by the apparent MLD signals. Along the northern part of the area with data coverage we see relics of Archean or younger northwest directed low-angle subduction below the entire Superior Craton. In the cratonic part of the US we see indications of the cratonic LAB near 200 km depth. In the Gulf Coast of the southern US, we image relics of southeast directed shallow subduction, likely of mid-Palaeozoic age.
Targeting Lymphocyte Activation Gene 3 to Reverse T-Lymphocyte Dysfunction and Improve Survival in Murine Polymicrobial Sepsis
Authors: Lou Jing-sheng , Wang Jia-feng , Fei Miao-miao , Zhang Yan , Wang Jun , Guo Yu , Bian Jin-jun , Deng Xiao-ming 
Published: April 2020

Abstract

Background
Lymphocyte activation gene 3 (LAG-3) is one of the immune checkpoint molecules, negatively regulating the T-cell reactions. The present study investigated the role of LAG-3 in sepsis-induced T-lymphocyte disability.

Methods
Mice sepsis was induced by cecal ligation and puncture (CLP). LAG-3 expression on some immune cells were detected 24 hours after CLP. LAG-3 knockout and anti–LAG-3 antibody were applied to investigate the effects on the survival, bacterial clearance. Cytokine levels, T-cell counts, and the presence of apoptosis (in blood, spleen, and thymus) were also determined. In vitro T-cell apoptosis, interferon γ secretion, and proliferation were measured. The expression of interleukin 2 receptor on T cells was also determined after CLP.

Results
LAG-3 was up-regulated on CD4+/CD8+ T, CD19+ B, natural killer, CD4+CD25+ regulatory T cells and dendritic cells. Both LAG-3 knockout and anti–LAG-3 antibody had a positive effect on survival and on blood or peritoneal bacterial clearance in mice undergoing CLP. Cytokine levels and T-cell apoptosis decreased in anti–LAG-3 antibody–treated mice. Induced T-cell apoptosis decreased, whereas interferon γ secretion and proliferation were improved by anti–LAG-3 antibody in vitro. Interleukin 2 receptor was up-regulated on T cells in both wild-type and LAG-3–knockout mice undergoing CLP.

Conclusions
LAG-3 knockout or anti–LAG-3 antibody blockade protected mice undergoing CLP from sepsis-associated immunodysfunction and may be a new target for the treatment.
Charged with Discharge: A Case-based Review of Nipple Discharge Using the American College of Radiology’s Appropriateness Guidelines
Authors: Karimova E Jane , Slanetz Priscilla J 
Published: April 2020

AbstractNipple discharge, a relatively common presenting symptom for women of all ages, may be due to both benign and malignant conditions. Men can also present with nipple discharge, and when they do, they have a higher likelihood of malignancy than women. Radiologists vary in their evaluation of patients with nipple discharge, although the American College of Radiology practice guidelines for nipple discharge provides data-driven appropriate algorithms. In patients with physiologic discharge, imaging is not typically indicated. For those with pathologic nipple discharge, imaging typically starts with diagnostic mammography and retroareolar ultrasound for women over 40 years of age, diagnostic mammogram or ultrasound for women aged 30–39 years, and ultrasound for women younger than 30 years. Finally, contrast-enhanced breast MRI or galactography are usually reserved for identifying the cause of discharge when initial imaging with mammography and ultrasound is unrevealing.
Managing Frozen Assets at the International Criminal Court
Authors: Birkett Daley J 
Published: April 2020

Abstract
On 8 June 2018, more than 10 years after his arrest, the Appeals Chamber of the International Criminal Court (ICC) reversed Jean-Pierre Bemba Gombo’s conviction by the Trial Chamber for crimes against humanity and war crimes, acquitting him of all charges. Soon after the start of his time in detention in The Hague, assets belonging to Bemba were frozen by states across a number of jurisdictions at the request of the ICC. Many of these assets remain frozen, more than 18 months after his acquittal. This article examines the consequences of prolonged asset freezes by the ICC through the lens of the Bemba case, demonstrating the existence of gaps in the legal framework applicable to the management of frozen assets under the ICC Statute system and suggesting possible responses thereto at the domestic and international levels.
Attractiveness of Cattle Dung to Coprophilous Beetles (Coleoptera: Scarabaeoidea and Sphaeridiinae) and Their Segregation During the Initial Stages of the Heterotrophic Succession on a Pasture in Southeast Michigan
Authors: Wassmer Thomas 
Published: April 2020

AbstractOnly a few mostly older studies analyzed the heterotrophic succession of dung beetles in the Midwestern United States. Such studies are needed to track the impacts of the climate crisis on heterotrophic succession and the associated decomposition processes that are central to soil fertility and carbon sequestration. The current study closes this knowledge gap and provides an easy and efficient method to estimate the relative attractiveness of individual dung pads during heterotrophic succession. The dung beetle community of Carpenter Farm in Adrian, Southeast Michigan was sampled for an entire year, including the winter months, using 15 pitfall traps baited with fresh cow manure. Samples were collected after 48 h and again after 72 h exposure time from the bucket content while leaving the bait unhampered. Eighty-four percent of all beetles were caught in the early sample, but only 6 species were missing in the later sample. A cluster analysis based on Pianka’s niche overlap identified a statistically higher mean overlap than expected by chance in a null model (model RA3) and divided the species community clearly into three clusters separating most relocators from most dwellers. Despite using a different method, my results confirmed the successional position of most previously described species and added data for several species with poor or unknown successional state. The successional segregation between dwellers and relocators discovered by the cluster analysis was paralleled by a significantly larger body size of relocators across taxonomic groups as compared to dwellers.
Selection following Gene Duplication Shapes Recent Genome Evolution in the Pea Aphid Acyrthosiphon pisum
Authors: Fernández Rosa , Marcet-Houben Marina , Legeai Fabrice , Richard Gautier , Robin Stéphanie , Wucher Valentin , Pegueroles Cinta , Gabaldón Toni , Tagu Denis 
Published: April 2020

Abstract
Ecology of insects is as wide as their diversity, which reflects their high capacity of adaptation in most of the environments of our planet. Aphids, with over 4,000 species, have developed a series of adaptations including a high phenotypic plasticity and the ability to feed on the phloem sap of plants, which is enriched in sugars derived from photosynthesis. Recent analyses of aphid genomes have indicated a high level of shared ancestral gene duplications that might represent a basis for genetic innovation and broad adaptations. In addition, there are a large number of recent, species-specific gene duplications whose role in adaptation remains poorly understood. Here, we tested whether duplicates specific to the pea aphid Acyrthosiphon pisum are related to genomic innovation by combining comparative genomics, transcriptomics, and chromatin accessibility analyses. Consistent with large levels of neofunctionalization, we found that most of the recent pairs of gene duplicates evolved asymmetrically, showing divergent patterns of positive selection and gene expression. Genes under selection involved a plethora of biological functions, suggesting that neofunctionalization and tissue specificity, among other evolutionary mechanisms, have orchestrated the evolution of recent paralogs in the pea aphid and may have facilitated host–symbiont cooperation. Our comprehensive phylogenomics analysis allowed us to tackle the history of duplicated genes to pave the road toward understanding the role of gene duplication in ecological adaptation.
Epidural Oscillating Cardiac-Gated Intracranial Implant Modulates Cerebral Blood Flow
Authors: Luciano Mark G , Dombrowski Stephen M , El-Khoury Serge , Yang Jun , Thyagaraj Suraj , Qvarlander Sara , Khalid Syed , Suk Ian , Manbachi Amir , Loth Francis 
Published: April 2020

Abstract

Background
We have previously reported a method and device capable of manipulating ICP pulsatility while minimally effecting mean ICP.

Objective
To test the hypothesis that different modulations of the intracranial pressure (ICP) pulse waveform will have a differential effect on cerebral blood flow (CBF).

Methods
Using an epidural balloon catheter attached to a cardiac-gated oscillating pump, 13 canine subjects underwent ICP waveform manipulation comparing different sequences of oscillation in successive animals. The epidural balloon was implanted unilaterally superior to the Sylvian sulcus. Subjects underwent ICP pulse augmentation, reduction and inversion protocols, directly comparing time segments of system activation and deactivation. ICP and CBF were measured bilaterally along with systemic pressure and heart rate. CBF was measured using both thermal diffusion, and laser doppler probes.

Results
The activation of the cardiac-gate balloon implant resulted in an ipsilateral/contralateral ICP pulse amplitude increase with augmentation (217%/202% respectively, P < .0005) and inversion (139%/120%, P < .0005). The observed changes associated with the ICP mean values were smaller, increasing with augmentation (23%/31%, P < .0001) while decreasing with inversion (7%/11%, P = .006/.0003) and reduction (4%/5%, P < .0005). CBF increase was observed for both inversion and reduction protocols (28%/7.4%, P < .0001/P = .006 and 2.4%/1.3%, P < .0001/P = .003), but not the augmentation protocol. The change in CBF was correlated with ICP pulse amplitude and systolic peak changes and not with change in mean ICP or systemic variables (heart rate, arterial blood pressure).

Conclusion
Cardiac-gated manipulation of ICP pulsatility allows the study of intracranial pulsatile dynamics and provides a potential means of altering CBF.
Epidemiology of synchronous brain metastases
Authors: Singh Raj , Stoltzfus Kelsey C , Chen Hanbo , Louie Alexander V , Lehrer Eric J , Horn Samantha R , Palmer Joshua D , Trifiletti Daniel M , Brown Paul D , Zaorsky Nicholas G 
Published: April 2020

Abstract

Background
The objectives of this study were to characterize (1) epidemiology of brain metastases at the time of primary cancer diagnosis, (2) incidence and trends of synchronous brain metastases from 2010 to 2015, and (3) overall survival (OS) of patients with synchronous brain metastases.

Methods
A total of 42 047 patients with synchronous brain metastases from 2010 to 2015 were identified from the Surveillance, Epidemiology, and End Results database. Descriptive analysis was utilized to analyze demographics and incidence. The Kaplan–Meier method and a Cox proportional hazards model were utilized to evaluate potential prognostic factors for OS.

Results
The majority of patients were diagnosed from age older than 50 (91.9%). Common primary sites included lung (80%), melanoma (3.8%), breast (3.7%), and kidney/renal pelvis (3.0%). Among pediatric patients, common primaries included kidney/renal pelvis and melanomas. The incidence was roughly 7.3 persons/100 000. Synchronous brain metastases were associated with significantly poorer OS compared to extracranial metastases alone (hazard ratio [HR] =1.56; 95% CI: 1.54–1.58; P &lt; .001). Among patients with brain metastases, male gender (HR = 1.60 vs 1.52), age older than 65 years (HR = 1.60 vs 1.46), synchronous liver, bone, or lung metastases (HR = 1.61 vs 1.49), and earlier year of diagnosis (HR = 0.98 for each year following 2010) were associated with significantly poorer OS.

Conclusions
The vast majority of brain metastases are from lung primaries. Synchronous brain metastases are associated with poorer OS compared to extracranial metastases alone.
Adherence to Treatment Guideline Improves Patient Outcomes in a Prospective Cohort of Adults Hospitalized for Community-Acquired Pneumonia
Authors: Lui Grace , To Heather K W , Lee Nelson , Chan Renee W Y , Li Timothy , Wong Rity Y K , Hui David S C , Ip Margaret 
Published: April 2020

Abstract

Background
Understanding local epidemiology and etiologies of community-acquired pneumonia in hospitalized patients is crucial for determining the appropriateness of treatment guidelines. We aim to determine the etiologies, severity, and outcomes in adults hospitalized for community-acquired pneumonia and to study the impact of empirical antibiotic therapy on patient outcomes.

Methods
We performed a prospective observational cohort study involving adults hospitalized for community-acquired pneumonia in Hong Kong. Sputum, nasopharyngeal aspirate, blood, and urine were collected for bacterial culture, molecular tests for detection of viruses and atypical pathogens, and antigen tests. Multivariable logistic regression model and Cox proportional hazard models were performed to determine independent factors associated with prolonged hospitalization and mortality.

Results
From February 2017 to July 2018, 258 patients were enrolled. The median age was 73 (interquartile range, 61–80) years, 66% were male, 57% had underlying chronic illnesses, 13% had CURB-65 score ≥3, and 10% had higher 1-year mortality. Pathogens were identified in 45% of patients; 20% had viral, 15% had bacterial, and 9% had polymicrobial pneumonia. Streptococcus pneumoniae (12%), influenza virus (12%), and Mycoplasma pneumoniae (1.2%) were the most common bacterial, viral, and atypical pathogens, respectively. Nonadherence to local empirical antibiotic treatment guidelines (primarily recommending beta-lactam and doxycycline) was observed in 25% and was independently associated with prolonged hospitalization (≥7 days) and higher mortality, after adjustment for age, underlying chronic illness, and disease severity.

Conclusions
Adherence to treatment guidelines was associated with shorter hospitalization and improved survival. We provided evidence for the use of doxycycline for coverage of atypical pathogens in nonsevere pneumonia.
Frailty Is Associated With Increased Hemagglutination-Inhibition Titers in a 4-Year Randomized Trial Comparing Standard- and High-Dose Influenza Vaccination
Authors: Loeb Nathalie , Andrew Melissa K , Loeb Mark , Kuchel George A , Haynes Laura , McElhaney Janet E , Verschoor Chris P 
Published: April 2020

Abstract

Background
Although high-dose (HD) vaccines have been reported to stimulate higher antibody responses compared with standard-dose (SD) influenza vaccines, there have been limited studies on the impact of frailty on such responses.

Methods
We conducted a randomized, double-blind trial (2014/2015 to 2017/2018) of SD versus HD trivalent split-virus vaccine (Fluzone) in 612 study participants aged 65+ over 4 influenza seasons. Hemagglutination inhibition antibody titers for influenza H1N1, H3N2, and B vaccine subtypes were measured at baseline and at 4, 10, and 20 weeks postvaccination and frailty was measured using a validated frailty index.

Results
Geometric mean antibody titers were significantly higher in HD compared with SD vaccine recipients for all influenza subtypes at all time points postvaccination. However, frailty was positively correlated with 4-week titers and was associated with increased odds of being a vaccine responder. For influenza A subtypes, this was mostly limited to HD recipients.

Conclusions
Frailty was associated with higher titers and increased antibody responses at 4 weeks after influenza vaccination, which was partially dependent on vaccine dosage. Chronic inflammation or dysregulated immunity, both of which are commonly observed with frailty, may be responsible, but it requires further investigation.
Studying in vivo dynamics of xylem-transported 11CO2 using positron emission tomography
Authors: Mincke Jens , Courtyn Jan , Vanhove Christian , Vandenberghe Stefaan , Steppe Kathy 
Published: April 2020

AbstractRespired CO2 in woody tissues can build up in the xylem and dissolve in the sap solution to be transported through the plant. From the sap, a fraction of the CO2 can either be radially diffuse to the atmosphere or be assimilated in chloroplasts present in woody tissues. These processes occur simultaneously in stems and branches, making it difficult to study their specific dynamics. Therefore, an 11C-enriched aqueous solution was administered to young branches of Populus tremula L., which were subsequently imaged by positron emission tomography (PET). This approach allows in vivo visualization of the internal movement of CO2 inside branches at high spatial and temporal resolution, and enables direct measurement of the transport speed of xylem-transported CO2 (vCO2). Through compartmental modeling of the dynamic data obtained from the PET images, we (i) quantified vCO2 and (ii) proposed a new method to assess the fate of xylem-transported 11CO2 within the branches. It was found that a fraction of 0.49 min−1 of CO2 present in the xylem was transported upwards. A fraction of 0.38 min−1 diffused radially from the sap to the surrounding parenchyma and apoplastic spaces (CO2,PA) to be assimilated by woody tissue photosynthesis. Another 0.12 min−1 of the xylem-transported CO2 diffused to the atmosphere via efflux. The remaining CO2 (i.e., 0.01 min−1) was stored as CO2,PA, representing the build-up within parenchyma and apoplastic spaces to be assimilated or directed to the atmosphere. Here, we demonstrate the outstanding potential of 11CO2-based plant-PET in combination with compartmental modeling to advance our understanding of internal CO2 movement and the respiratory physiology within woody tissues.
Timeline of autumn phenology in temperate deciduous trees
Authors: Dox Inge , Gričar Jožica , Marchand Lorène J , Leys Sebastien , Zuccarini Paolo , Geron Charly , Prislan Peter , Mariën Bertold , Fonti Patrick , Lange Holger , Peñuelas Josep , Van den Bulcke Jan , Campioli Matteo 
Published: April 2020

Abstract
Cessation of xylem formation or wood growth (CWG) and onset of foliar senescence (OFS) are key autumn phenological events in temperate deciduous trees. Their timing is fundamental for the development and survival of trees, ecosystem nutrient cycling and the seasonal exchange of matter and energy between the biosphere and atmosphere, and affects the impact and feedback of forests to global change. A large-scale experimental effort and improved observational methods have allowed us to compare the timing of CWG and OFS for different deciduous tree species in Western Europe, particularly in silver birch, a pioneer species, and European beech, a late-succession species, at stands of different latitudes, of different levels of site fertility, for 2 years with contrasting meteorological and drought conditions, i.e., the low moderately dry 2017 and the extremely dry 2018. Specifically, we tested whether foliar senescence started before, after or concurrently with CWG. Onset of foliar senescence and CWG occurred generally between late September and early November, with larger differences across species and sites for OFS. Foliar senescence started concurrently with CWG in most cases, except for the drier 2018 and, for beech, at the coldest site, where OFS occurred significantly later than CWG. The behavior of beech in Spain, the southern edge of its European distribution, was unclear, with no CWG, but very low wood growth at the time of OFS. Our study suggests that OFS is generally triggered by the same drivers of CWG or when wood growth decreases in late summer, indicating an overarching mechanism of sink limitation as a possible regulator of the timing of foliar senescence.
Early epidemiological and clinical manifestations of COVID-19 in Japan
Authors: Qasim Muhammad , Yasir Muhammad , Ahmad Waqas , Yoshida Minami , Azhar Muhammad , Ali Mohammad Azam , Wang Chris , Gould Maree 
Published: April 2020

AbstractBackgroundSevere acute respiratory syndrome coronaviruses -2 (SARS-COV2) named as COVID-19 had spread worldwide and leading to 1,210,956 confirmed cases and 67,594 deathsMethodsA data of 1192 confirmed cases and 43 deaths due to COVID-19 in Japan collected from the Ministry of Health, Labour and Welfare of Japan and analysed for different epidemiological parameters and their clinical manifestations. We used Clauset-Newman-Moore (CNM) clustering algorithm to develop web-network of confirmed cases to identified clusters of community transmission.ResultsOut of 1192 confirmed cases, 90.60% were symptomatic and 9.39% were asymptomatic. The prevalence of COVID19 in males was 56.29% and 43.20 % in females. The mean interval (±SD) from symptom onset to diagnosis was 6±22.6 days while mean interval (±SD) from contact to onset of symptoms was 5±19.5 days. People of age range 40-79 were more infected and deaths median age was 80. The main symptoms were fever, dry cough, fatigue and pneumonia. The main infected cities were Tokyo (195/1192, 16.35%), Hokkaido (160/1192 13.42%), Aichi (150/1192, 12.58%) and Osaka (145/1192, 12.16%). Only 2.34% cases had travel history from Wuhan China and Osaka music concert was identify as main cluster for community transmission. While 556 (46.64%) cases were clinically diagnosed and 557 (46.72%) were confirmed by using RT-PCR.ConclusionsOther than, declare emergency Japan need to change their approach of diagnosing COVID-19, as asymptomatic cases prevalence is high and maybe it is reason for current sudden increase of cases. Screening centre should be establish away from hospitals, which are treating positive cases.