bluuebunny/crossref_metadata_2025_split huggingface dataset:
Playing Hide and Seek with ‘Vergangenheit, die nicht vergehen will’ (‘a Past that Will Not Pass’) in the History of International Law
Authors: de la Rasilla Ignacio 
Published: November 2019

Abstract
The chapter proceeds in two parts. The first reviews the enactment of the Spanish Historical Memory Act (HMA) and examines three of its most polemical areas of implementation in the light of the concerns raised by several United Nations human rights bodies charged with overseeing the conflict-ridden politics of historical memory in Spain. The second part of the chapter builds on Spanish experience in examining some of the most common hurdles, including methodological ones, that an incoming democratic generation of international lawyers may face when attempting a balanced exploration of the history of international law after a long period of authoritarian rule in their home country.
Statehood and the State-Like in International Law
Authors: Nicholson Rowan 
Published: November 2019

AbstractIf the term were given its literal meaning, international law would be law between ‘nations’. It is often described instead as being primarily between states. But this conceals the diversity of the nations or state-like entities that have personality in international law or that have had it historically. This book reconceptualizes statehood by positioning it within that wider family of state-like entities. An important conclusion of the book is that states themselves have diverse legal underpinnings. Practice in cases such as Somalia and broader principles indicate that international law provides not one but two alternative methods of qualifying as a state: subject to exceptions connected with territorial integrity and peremptory norms, an entity can be a state either on the ground that it meets criteria of effectiveness or on the ground that it is recognized by all other states. Another conclusion is that states, in the strict legal sense in which the word is used today, have never been the only state-like entities with personality in international law. Others from the past and present include imperial China in the period when it was unreceptive to Western norms; pre-colonial African chiefdoms; ‘states-in-context’, an example of which may be Palestine, which have the attributes of statehood relative to states that recognize them; and entities such as Hong Kong.
Other state-like entities in a world of states
Authors: Nicholson Rowan 
Published: November 2019

AbstractThis chapter looks at the international legal personality of state-like entities that exist today other than states in the strict sense and states-in-context. These include including entities in free association, subentities of states such as Hong Kong, and the Holy See. It concludes that, despite the dominance of states, international law allows other state-like entities to acquire personality in two ways: ‘volitionally’ (usually under a treaty or by recognition) or ‘automatically’ (usually by direct operation of a customary norm). The chapter also serves to corroborate the conclusion, drawn elsewhere in the book, that there are two coexisting methods of acquiring statehood: effectiveness and recognition.
Structure, function, and evolution of respiratory proteins
Authors: Perry Steven F. , Lambertz Markus , Schmitz Anke 
Published: November 2019

Abstract
Respiratory proteins are complexes of proteins and metal ions. In haemoglobin the metal is iron, in haemocyanin—the most common invertebrate respiratory protein—it is copper. Globins such as haemoglobin and myoglobin and related molecular complexes have probably been around as long as life itself, whereas others such as the most common respiratory protein of molluscs and arthropods, haemocyanin, appear to be younger and are not chemically related to globins. Nevertheless, astounding functional similarities between haemoglobin and haemocyanin are seen. The present chapter takes a look at the molecular mechanisms behind their function, their fundamental integration in the respiratory process, and also traces the evolution of these respiratory proteins.
The evolution of air-breathing respiratory faculties in invertebrates
Authors: Perry Steven F. , Lambertz Markus , Schmitz Anke 
Published: November 2019

Abstract
This chapter aims at piecing together the evolution of air breathing in invertebrates, the main conclusion here being that it evolved independently several times. In molluscs alone, air breathing has evolved several times, but almost exclusively among snails. Among crustaceans, several groups of crabs have also independently developed terrestrial representatives and transitional stages, particularly in the control of breathing, are evident. Analysis of insects shows few recognizable evolutionary progressions: air sacs and different stigmatal closure mechanisms have appeared and disappeared numerous times, even within closely related groups. But other tracheate groups such as myriapods show an interesting correlation between the presence of tracheal lungs, which end in an open circulatory system, and tracheae that invade the tissue as in insects, and the presence or reduction of respiratory proteins. In arachnids a similar tendency is seen, and the most interesting developments were the (partial) replacement of a ‘perfectly good’ air-breathing organ (book lungs) by another one (tracheae).
Facilitating Injustice
Authors: Park Yoosun 
Published: November 2019

Abstract
Social workers were involved in all aspects of the removal, incarceration, and resettlement of the Nikkei, a history that has been forgotten by social work. This study is an effort to address this lacuna. Social work equivocated. While it did not fully endorse mass removal and incarceration, neither did it protest, oppose, or explicitly critique government actions. The past should not be judged by today’s standards; the actions and motivations described here occurred in a period rife with fear and propaganda. Undergoing a major shift from its private charity roots into its public sector future, social work bounded with the rest of society into “a patriotic fervor.” While policies of a government at war, intractable bureaucratic structures, tangled political alliances, and complex professional obligations all may have mandated compliance, it is, nevertheless, difficult to deny that social work and social workers were also willing participants in the events, informed about and aware of the implications of that compliance. In social work’s unwillingness to take a resolute stand against removal and incarceration, the well-intentioned profession, doing its conscious best to do good, enforced the existing social order and did its level best to keep the Nikkei from disrupting it.
Genome-wide characterization of copy number variations in diffuse large B-cell lymphoma with implications in targeted therapy
Authors: Dharanipragada Prashanthi , Parekh Nita 
Published: November 2019

Abstract
Diffuse large B-cell lymphoma (DLBCL) is the aggressive form of haematological malignancies with relapse/refractory in ~ 40% of cases. It mostly develops due to accumulation of various genetic and epigenetic variations that contribute to its aggressiveness. Though large-scale structural alterations have been reported in DLBCL, their functional role in pathogenesis and as potential targets for therapy is not yet well understood. In this study we performed detection and analysis of copy number variations (CNVs) in 11 human DLBCL cell lines (4 activated B-cell–like [ABC] and 7 germinal-centre B-cell–like [GCB]), that serve as model systems for DLBCL cancer cell biology. Significant heterogeneity observed in CNV profiles of these cell lines and poor prognosis associated with ABC subtype indicates the importance of individualized screening for diagnostic and prognostic targets. Functional analysis of key cancer genes exhibiting copy alterations across the cell lines revealed activation/disruption of ten potentially targetable immuno-oncogenic pathways. Genome guided in silico therapy that putatively target these pathways is elucidated. Based on our analysis, five CNV-genes associated with worst survival prognosis are proposed as potential prognostic markers of DLBCL.
Statistical shape modelling provides a responsive measure of morphological change in knee osteoarthritis over 12 months
Authors: Gregory Jennifer S , Barr Rebecca J , Yoshida Kanako , Alesci Salvatore , Reid David M , Aspden Richard M 
Published: November 2019

Abstract

Objectives
Responsive biomarkers are needed to assess the progression of OA and their lack has hampered previous clinical trials. Statistical shape modelling (SSM) from radiographic images identifies those at greatest risk of fast-progression or joint replacement, but its sensitivity to change has not previously been measured. This study evaluates the responsiveness of SSM in knee OA in a 12-month observational study.

Methods
A total of 109 people were recruited who had undergone knee radiographs in the previous 12 months, and were grouped based on severity of radiographic OA (Kellgren–Lawrence grading). An SSM was built from three dual-energy X-ray absorptiometry scans at 6-month intervals. Change-over-time and OA were assessed using generalized estimating equations, standardized response means (SRM) and reliable change indices

Results
Mode 1 showed typical features of radiographic OA and had a strong link with Kellgren–Lawrence grading but did not change significantly during the study. Mode 3 showed asymmetrical changes consistent with medial cartilage loss, osteophytes and joint malalignment, and was responsive to change, with a 12-month SRM of 0.63. The greatest change was observed in the moderate radiographic OA group (SRM 0.92) compared with the controls (SRM 0.21), and the reliable change index identified 14% of this group whose progression was clinically significant.

Conclusion
Shape changes linked the progression of osteophytosis with increasing malalignment within the joint. Modelling of the whole joint enabled quantification of change beyond the point where bone-to-bone contact has been made. The knee SSM is, therefore, a responsive biomarker for radiographic change in knees over 12 months.
Elevated temperature and CO2 cause differential growth stimulation and drought survival responses in eucalypt species from contrasting habitats
Authors: Apgaua Deborah M G , Tng David Y P , Forbes Samantha J , Ishida Yoko F , Vogado Nara O , Cernusak Lucas A , Laurance Susan G W 
Published: November 2019

AbstractClimate change scenarios predict increasing atmospheric CO2 concentrations ([CO2]), temperatures and droughts in tropical regions. Individually, the effects of these climate factors on plants are well established, whereas experiments on the interactive effects of a combination of factors are rare. Moreover, how these environmental factors will affect tree species along a wet to dry gradient (e.g., along tropical forest–savanna transitions) remains to be investigated. We hypothesized that under the simulated environmental conditions, plant growth, physiological performance and survivorship would vary in a manner consistent with the species’ positions of origin along this gradient. In a glasshouse experiment, we raised seedlings of three Eucalyptus species, each occurring naturally in a wet forest, savanna and forest–savanna ecotone, respectively. We evaluated the effect of drought, elevated temperature (4 °C above ambient glasshouse temperature of 22 °C) and elevated temperature in combination with elevated [CO2] (400 ppm [CO2] above ambient of 400 ppm), on seedling growth, survivorship and physiological responses (photosynthesis, stomatal conductance and water-use efficiency). Elevated temperature under ambient [CO2] had little effect on growth, biomass and plant performance of well-watered seedlings, but hastened mortality in drought-affected seedlings, affecting the forest and ecotone more strongly than the savanna species. In contrast, elevated [CO2] in combination with elevated temperatures delayed the appearance of drought stress symptoms and enhanced survivorship in drought-affected seedlings, with the savanna species surviving the longest, followed by the ecotone and forest species. Elevated [CO2] in combination with elevated temperatures also enhanced growth and biomass and photosynthesis in well-watered seedlings of all species, but modified shoot:root biomass partitioning and stomatal conductance differentially across species. Our study highlights the need for a better understand of the interactive effects of elevated [CO2], temperature and drought on plants and the potential to upscale these insights for understanding biome changes.
Is liver glycogen fragility a possible drug target for diabetes?
Authors: Li Cheng , Hu Zhenxia 
Published: November 2019

AbstractLiver glycogen α particles are molecularly fragile in diabetic mice, and readily form smaller β particles, which degrade more rapidly to glucose. This effect is well associated with the loss of blood‐glucose homeostasis in diabetes. The biological mechanism of such fragility is still unknown; therefore, there are perceived opportunities that could eventually lead to new means to manage type 2 diabetes. The hierarchical structures of glycogen particles are controlled by the underlying biosynthesis/degradation process that involves various enzymes, including, for example, glycogen synthase (GS) and glycogen‐branching enzyme (GBE). Recent studies have shown that fragile glycogen α particles in diabetic mice have longer chains and a higher molecular density compared to wild‐type mice, indicating an enhanced enzymatic activity ratio of GS to GBE in diabetes. Furthermore, it has been shown that with an improved blood glucose homeostasis, the glycogen fragility in diabetic mice can be restored by treatment with active ingredients from traditional Chinese medicine, yet the underlying mechanism is unknown. In this review, we summarize recent advances in understandings glycogen fragility from the perspectives of glycogen biosynthesis/degradation, glycogen hierarchical structures, and its relation to diabetes. Importantly, we for the first time set GS/GBE activity ratio as the therapeutic target for diabetes.
Haliea alexandrii sp. nov., isolated from phycosphere microbiota of the toxin-producing dinoflagellate Alexandrium catenella
Authors: Yang Qiao , Jiang Zhiwei , Zhou Xin , Zhang Ruonan , Xie Zhangxian , Zhang Shuo , Wu Yiru , Ge Yaming , Zhang Xiaoling 
Published: November 2019

A Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped bacterium, named strain LZ-16-2T, was isolated from the phycosphere microbiota of the paralytic shellfish poisoning toxin-producing marine dinoflagellate Alexandrium catenella LZT09. Strain LZ-16-2T grew optimally at 28 °C at pH 6.5 and with 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain LZ-16-2T fell within the genus

Haliea

and was most closely related to

Haliea salexigens

DSM 19537T, with which the new isolate exhibited 98.5 % 16S rRNA gene sequence similarity. The major respiratory quinone was Q-8. The predominant cellular fatty acids were C17 : 1
ω8c, summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c), summed feature 8 (C18 : 1
ω7c and/or C18 : 1
ω6c), C17 : 1
ω6c, C11 : 0 3-OH and C17 : 0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The average nucleotide identity and in silico DNA–DNA genome hybridization relatedness values between strain LZ-16-2T and its closest relative,

H. salexigens

DSM 19537T, were 92.8 and 55.1 %, respectively. The DNA G+C content was 61.3 mol%. Differential phenotypic properties and phylogenetic distinctiveness distinguished strain LZ-16-2T from all other members of the genus

Haliea

. On the basis of the polyphasic characterization, strain LZ-16-2T represents a novel species of the genus

Haliea

, for which the name Haliea alexandrii sp. nov. is proposed. The type strain is LZ-16-2T (=KCTC 62344T=CCTCC AB2017229T).
Investigation of working memory networks for verbal and rhythmic stimuli
Authors: Hoddinott Joshua D. , Schuit Dirk , Grahn Jessica A. 
Published: November 2019

AbstractAuditory working memory is often conceived of as a unitary capacity, with memory for different auditory materials (syllables, pitches, rhythms) thought to rely on similar neural mechanisms. One spontaneous behavior observed in working memory studies is ‘chunking’. For example, individuals often recount digit sequences in groups, or chunks, of 3 to 4 digits, and this chunking improves performance. Chunking may also operate in musical rhythm, with beats acting as chunk boundaries for tones in rhythmic sequences. Similar to chunking, beat-based structure in rhythms also improves performance. Thus, beat processing may rely on the same mechanisms that underlie chunking of verbal material. The current fMRI study examined whether beat perception is a type of chunking, measuring brain responses to chunked and unchunked letter sequences relative to beat-based and nonbeat-based rhythmic sequences. Participants completed a sequence discrimination task, and comparisons between stimulus encoding, maintenance, and discrimination were made for both rhythmic and verbal sequences. Overall, rhythm and verbal working memory networks overlapped substantially. When comparing rhythmic and verbal conditions, rhythms activated basal ganglia, supplementary motor area, and anterior insula, compared to letter strings, during encoding and discrimination. Letter strings compared to rhythms activated bilateral auditory cortex during encoding, and parietal cortex, precuneus, and middle frontal gyri during discrimination. Importantly, there was a significant interaction in the basal ganglia during encoding: activation for beat-based rhythms was greater than for nonbeat-based rhythms, but verbal chunked and unchunked conditions did not differ. The significant interaction indicates that beat perception is not simply a case of chunking, suggesting a dissociation between beat processing and grouping mechanisms that warrants further exploration.
Stereological characterization of neurogenic niche secretory cell types in the mouse dorsal dentate gyrus
Authors: Rieskamp Joshua D. , Sarchet Patricia , Smith Bryon M. , Kirby Elizabeth D. 
Published: November 2019

AbstractAdult neurogenesis in the dorsal dentate gyrus (DG) subregion of the mammalian hippocampus supports critical cognitive processes related to memory. Local DG cell populations form a neurogenic niche specialized to regulate adult neurogenesis. Recently, DG astrocytes, microglia, endothelia, and neural stem cells have been identified as sources of neurogenesismodulating secreted factors. Accurately estimating the size of these cell populations is useful for elucidating their relative contributions to niche physiology. Previous studies have characterized these cell types individually, but to our knowledge no comprehensive study of all these cell types exists. This is problematic because considerable variability in reported population size complicates comparisons across studies. Here, we apply consistent stereological methods within a single study to estimate cell density for neurogenesis-modulating secretory cell types in the dorsal DG of adult mice. We used immunohistochemical phenotypic markers to quantify cell density and found that stellate astrocytes were the most numerous followed by endothelia, intermediate progenitors, microglia, and neural stem cells. We did not observe any significant sex differences in cell density. We expect our data will facilitate efforts to elucidate the role of DG secretory cell populations in regulating adult neurogenesis.
Linked networks reveal dual roles of insect dispersal and species sorting for bacterial communities in flowers
Authors: Zemenick Ash T. , Vannette Rachel L. , Rosenheim Jay A. 
Published: November 2019

AbstractDue to the difficulty of tracking microbial dispersal, it rarely possible to disentangle the relative importance of dispersal and species sorting for microbial community assembly. Here, we leverage a detailed multilevel network to examine drivers of bacterial community assembly within flowers. We show that plant species with similar visitor communities tend to have similar bacterial communities, and visitor identity to be more important than dispersal rate in structuring floral bacterial communities. However, plants occupied divergent positions in plant-insect and plant-microbe networks, suggesting an important role for species sorting. Taken together, our analyses suggest dispersal is important in determining similarity in microbial communities across plant species, but not as important in determining structural features of the floral bacterial network. A multilevel network approach thus allows us to address features of community assembly that cannot be considered when viewing networks as separate entities.
An asymmetric sheath controls flagellar supercoiling and motility in the Leptospira spirochete
Authors: Gibson Kimberley H. , Trajtenberg Felipe , Wunder Elsio A. , Brady Megan R. , San Martin Fabiana , Mechaly Ariel E. , Shang Zhiguo , Liu Jun , Picardeau Mathieu , Ko Albert I. , Buschiazzo Alejandro , Sindelar Charles V. 
Published: November 2019

AbstractSpirochete bacteria, including important pathogens, exhibit a distinctive means of swimming via undulations of the entire cell. Motility is powered by the rotation of supercoiled ‘endoflagella’ that wrap around the cell body, confined within the periplasmic space. To investigate the structural basis of flagellar supercoiling, which is critical for motility, we determined the structure of native flagellar filaments from the spirocheteLeptospiraby integrating high-resolution cryo-electron tomography and X-ray crystallography. We show that these filaments are coated by a highly asymmetric, multi-component sheath layer, contrasting with flagellin-only homopolymers previously observed in exoflagellated bacteria. Distinct sheath proteins localize to the filament inner and outer curvatures to define the supercoiling geometry, explaining a key functional attribute of the spirochete flagellum.One Sentence summaryThe corkscrew-like motility of Spirochete bacteria is enabled by a unique, asymmetrically constructed flagellum that wraps around the cell body within the periplasm.
Pits and CtBP control tissue growth in Drosophila melanogaster with the Hippo pathway transcription repressor, Tgi
Authors: Vissers Joseph H.A. , Dent Lucas G. , House Colin , Kondo Shu , Harvey Kieran F. 
Published: November 2019

ABSTRACTThe Hippo pathway is an evolutionary conserved signalling network that regulates organ size, cell fate control and tumorigenesis. In the context of organ size control, the pathway incorporates a large variety of cellular cues such as cell polarity and adhesion into an integrated transcriptional response. The central Hippo signalling effector is the transcriptional co-activator Yorkie, which controls gene expression in partnership with different transcription factors, most notably Scalloped. When it is not activated by Yorkie, Scalloped can act as a repressor of transcription, at least in part due to its interaction with the corepressor protein Tgi. The mechanism by which Tgi represses transcription is incompletely understood and therefore we sought to identify proteins that potentially operate together with it. Using an affinity purification and mass-spectrometry approach we identified Pits and CtBP as Tgi-interacting proteins, both of which have been linked to transcriptional repression. Both Pits and CtBP were required for Tgi to suppress the growth of the D. melanogaster eye and CtBP loss suppressed the undergrowth of yorkie mutant eye tissue. Furthermore, as reported previously for Tgi, overexpression of Pits suppressed transcription of Hippo pathway target genes. These findings suggest that Tgi might operate together with Pits and CtBP to repress transcription of genes that normally promote tissue growth. The human orthologues of Tgi, CtBP and Pits (VGLL4, CTBP2 and IRF2BP2) physically and functionally interact to control transcription, implying that the mechanism by which these proteins control transcriptional repression is conserved throughout evolution.