bluuebunny/crossref_metadata_2025_split huggingface dataset:
The desmosomal cadherin Desmoglein-3 regulates YAP and phospho-YAP in keratinocyte responses to mechanical forces
Authors: Uttagomol Jutamas , Ahmad Usama Sharif , Rehman Ambreen , Huang Yunying , Laly Ana C. , Kang Angray , Soetaert Jan , Chance Randy , Teh Muy-Teck , Connelly John T. , Wan Hong 
Published: November 2019

ABSTRACTDesmoglein 3 (Dsg3), plays a crucial role in cell-cell adhesion and tissue integrity. Increasing evidence suggests that Dsg3 acts as a regulator of cellular mechanotransduction, but little is known about its direct role in mechanical force transmission. The present study investigated the impact of cyclic strain and substrate stiffness on Dsg3 expression and its role in mechanotransduction. A direct comparison was made with E-cadherin, a well-characterized mechanosensor, in human keratinocytes. Exposure of oral and skin keratinocytes to equiaxial cyclic strain promoted changes in expression and localization of junction assembly proteins. Knockdown of Dsg3 by siRNA blocked strain-induced junctional remodeling of E-cadherin and Myosin IIa. Importantly, the study demonstrated that Dsg3 regulates the expression and localization of YAP, a mechanosensor and an effector of the Hippo pathway. Furthermore, we showed that Dsg3 forms a complex with phospho-YAP and sequestered it to the plasma membrane, while Dsg3 depletion had an impact on both YAP and phospho-YAP in their response to mechanical forces, increasing the sensitivity of keratinocytes to both strain-or substrate rigidity-induced nuclear relocalization of YAP and phospho-YAP. We showed that PKP1 seemed to be the key in such a complex formation since its silencing resulted in Dsg3 disruption at the junctions with concomitant loss of pYAP in the peripheral cytoplasm. Finally, we demonstrated that this Dsg3/YAP pathway has an influence on the expression ofYAP1target genes as well as cell proliferation in keratinocytes. Together, these findings provide evidence of a novel role for Dsg3 in keratinocyte mechanotransduction.
GPSRdocker: A Docker-based Resource for Genomics, Proteomics and Systems biology
Authors: Agrawal Piyush , Kumar Rajesh , Usmani Salman Sadullah , Dhall Anjali , Patiyal Sumeet , Sharma Neelam , Kaur Harpreet , Kumar Vinod , Kaur Dilraj , Jain Shipra , Pande Akshara , Bhalla Sherry , Raghava Gajendra P.S. 
Published: November 2019

AbstractBackgroundIn past number of web-based resources has been developed in the field of Bioinformatics. These resources are heavily used by scientific community to provide solution for challenges faced by experimental researchers particularly in the field of biomedical sciences. There are number of challenges in utilizing full potential of these services that includes internet speed, limits on computing power, and security of data. In order to enhance utilities of these web-based assets, we developed a docker-based container that integrates large number resources available in literature.ResultsThis paper describes GPSRdocker a docker-based container developed for providing wide-range of computational tools in the field of bioinformatics particularly in genomics, proteomics and system biology. Majority of tools integrated in GPSRdocker are based on web services developed at Raghava’s group in last two decades. Broadly, these tools can be categorized in three categories; i) general scripts, ii) supporting software and iii) major standalone software. In order to facilitate students or developers working in the field of bioinformatics, we developed general scripts in Perl and Python. These general-purpose scripts serve as building block for any bioinformatics tools like computing features/descriptors of a protein. Supporting software packages includes SCIKIT, WEKA, SVMlight, and PSI-BLAST; these software packages allow one to develop/implement bioinformatics software. Major Standalone software is core of this container which allows predicting function/class of biomolecules. These tools can be classified broadly in following categories; protein annotation, epitope-based vaccines, prediction of interaction and drug discovery.ConclusionA docker-based container has been developed which can be easily run on any operating system as well as it can be directly ported on cloud. Scripts can be run to build pipelines for addressing problems at system level like prediction of vaccine candidate for a pathogen. GPSRdocker including manual is available free for academic use from https://webs.iiitd.edu.in/gpsrdocker.
Development of anin vitroExperimental Model for Investigating the Effect of Matrix Stiffness on Epithelial Barrier Permeability
Authors: Roy Neilloy , Turner-Brannen Emily , West Adrian R 
Published: November 2019

ABSTRACTEpithelial cells are well-known to be modulated by extracellular mechanical factors including substrate stiffness. However, the effect of substrate stiffness on an epithelial cell’s principal function –creating an effective barrier to protect the underlying tissue – cannot be directly measured using existing experimental techniques. We developed a strategy involving ethylenediamine aminolysis and glutaraldehyde crosslinking to chemically graft polyacrylamide hydrogels with tunable stiffness to PET Transwell membranes. Grafting success was evaluated using spectroscopic methods, scrape tests, and extended incubation in culture. By assessing apical to basolateral transfer of fluorescent tracers, we demonstrated that our model is permeable to biologically relevant molecules and usable for direct measurement of barrier function by calculating paracellular permeability.We found that BEAS-2B epithelial cells form a more effective barrier on stiff substrates, likely attributable to increased cell spreading. We also observed barrier impairment after treatment with transforming growth factor beta, indicating loss of cell-cell junctions, validating our model’s ability to detect biologically relevant stimuli. Thus, we have created an experimental model that allows explicit measurement of epithelial barrier function for cells grown on different substrate stiffnesses. This model will be a valuable tool to study mechanical regulation of epithelial and endothelial barrier function in health and disease.
Profiling the effects of rifaximin on the healthy human colonic microbiota using a chemostat model
Authors: Moura Ines B. , Buckley Anthony M. , Ewin Duncan , Clark Emma , Mitra Suparna , Wilcox Mark H. , Chilton Caroline H. 
Published: November 2019

AbstractRifaximin is a low solubility antibiotic with activity against a wide range of bacterial pathogens. It accumulates in the intestine and is suitable for prolonged use. Three chemostat models (A, B and C) were used to investigate the effects of three rifaximin formulations (α, β and κ, respectively) on the gut microbiome. Bacterial populations were monitored by bacterial culture and 16S rRNA gene amplicon (16S) sequencing. Limited disruption of bacterial populations was observed for rifaximin α, β and κ. All formulations caused declines in total spores (∼2 log10 cfu ml-1), Enterococcus spp. (∼2 log10 cfu ml-1 in models A and C, and ∼1 log10 cfu ml-1 in model B), and Bacteroides spp. populations (∼3 log10 cfu ml-1 in models A and C, and ∼4 log10 cfu ml-1 in model B). Bacterial populations fully recovered during antibiotic dosing in model C, and before the end of the experiment in models A and B. According to the taxonomic analysis, prior to rifaximin exposure, Bifidobacteriaceae, Ruminococcaceae, Acidaminococcaceae, Lachnospiraceae and Rikenellaceae families represented >92% of the total relative abundance, in all models. Within these families, 15 bacterial genera represented >99% of the overall relative abundance. Overall, the 16S sequencing and culture data showed similar variations in the bacterial populations studied. Among the three formulations, rifaximin κ appeared to have the least disruptive effect on the colonic microbiota, with culture populations showing recovery in a shorter period and the taxonomic analysis revealing the least global variation in relative abundance of prevalent groups.
A technology roadmap study on omni-channel library
Authors: Yu Hueiju , Chang Yu-Ting , Chu Chih-Ying 
Published: November 2019

Purpose
The purpose of the paper is to: check both of the product and technical items and propose a technology classification framework with a roadmap for omni-channel library; draw the technical association diagram and maturation of the time of developing the omni-channel library; predict the trends of omni-channel library development architecture.

Design/methodology/approach
The method to carry out this study were secondary data analysis and experts’ interviews. The data were collected 80 cases of omni-channel and academic literature, then identified and drawn as a technology roadmap. Furthermore, smart technology experts and researchers were interviewed to not only examine and define the technical association diagram but also predict the market and technology maturation time and predict the trends of omni-channel library.

Findings
Owing to the continuous improvement of smart technologies, technology channels of delivering digital content have been changed. Content providers will confront numerous challenges in the future. There are several literature and practice findings in this paper. In addition to building a roadmap, technical association diagram and maturation time for omni-channel library, based on market integration and product value, this paper proposes an omni-channel library development architecture.

Originality/value
The results of this study can be used as practical applications in developing library channel integration strategy. In addition, this study provides insights into future studies in the field of digital content channel.
Exploring the wine sector in the Nashik district of India
Authors: Kansara Surendra 
Published: November 2019

Purpose
In India, Nashik District of Maharashtra State is a major Indian wine-producing region. This study aims to critically explore multiple aspects of an area and bring to the forefront of various sociological or educational variables (Kerlinger, 1973). The variables can help in the generation of the conjectures and premises.

Design/methodology/approach
Methodologically, to explore the wine sector in the Nashik District of India total 304 number of wine products and its pricing along with a total 26 number of wineries attributes has evaluated by collecting details of different product attributes of wine products and retail pricing. These were regressed with backward integration to arrive at the characteristics that govern wine prices. Concerning their motivation for wine tourism, a survey of 197 visitors conducted. Seven winery owners/ officials were interviewed that includes the Coordinator of the Wineries association. The interview schedule included open-ended questions. Thus, providing more in-depth insight into the wine sector in the Nashik District of India.

Findings
An investigation of the wine industry in the Nashik district of India suggests the potential for combined effects between wineries, local communities and the economy by discovering the sociological or educational variables associated with the considered geographic region.

Research limitations/implications
The research is the field study aimed at discovering the relations or interactions among sociological or educational variables. There can be variables beyond the considered variables affecting the wine product mix and pricing, wine tourism motivation factors and the factors responsible for the economic development of the region.

Originality/value
The empirical research throws light on an unexplored wine-producing region and its potential impact on livelihood. The exploratory study discovers the interactions among educational variables, as asserted by Kerlinger (1973). It can also help in the generation of premises about the wine sector from the area.
Aggression in Iranian adolescent girls: role of depressive mood, dysphoric disorder and premenstrual syndrome
Authors: Rezaei Sajjad 
Published: November 2019

Purpose
Aggression is a destructive experience in terms of social and public health. The purpose of this paper is to determine the role of depressive mood, premenstrual dysphoric disorder (PMDD) and premenstrual syndrome (PMS) in adolescent girls’ aggression.

Design/methodology/approach
In a cross-sectional study, 510 girl students were selected by multistage cluster sampling from Lahijan and Sangar high schools (Northern Iran) in the 2017–2018 academic year, and each of them responded to the short version of Beck Depression Inventory (BDI-13), Premenstrual Symptoms Screening Tool and Ahvaz Aggression Inventory. Data were analyzed by point-biserial and Pearson’s correlation coefficients, univariate analysis of covariance in the form of 2 × 3 factorial design and Hochberg’s GT2 post hoc test.

Findings
The questionnaires of 475 students were returned correctly (survey validity=93 percent). The results of ANCOVA after adjustment for confounding variables such as age and physical illness history revealed that the existence of main effect for depressive mood (F=31.50, df=1, p<0.0001) and PMS and PMDD diagnoses (F=11.39, df=2, p<0.0001) were associated with increased aggression. However, there was no significant interaction effect on aggression levels (p>0.05). Additionally, post hoc tests revealed no significant differences between the diagnosis of PMS and PMDD in terms of aggression (p>0.05).

Research limitations/implications
The present study has some limitations. Depressive mood and diagnoses of PMS and PMDD were defined through relying on the self-report data and cut points suggested by the questionnaires. Obviously, change of measurement tools or even cut points reduces the results reliability and repeatability. Furthermore, the research plan does not allow us to infer causal relations and does not provide information about the direction of the relationship between depression symptoms, PMS and PMDD diagnoses, and aggression. Finally, the present study is relied on high schools’ data, and the results cannot be generalized to other adolescent girls.

Originality/value
Despite the limitations of this study, its findings offer new insights into the factors influencing the perpetration of aggression in Iranian adolescent girls. Depressed adolescent girls and those receiving a PMS or PMDD diagnosis are more likely to develop aggression. These findings can be used in high schools to design educational and health-based interventions in order to reduce and prevent anger and resentment in adolescent girls.
Business models in facilities management value chains
Authors: Jensen Per Anker 
Published: November 2019

Purpose
This paper aims to identify typical sourcing strategies and business models in facilities management (FM) and map archetypes of value chains with complementary sourcing strategies and value chains.

Design/methodology/approach
The paper is based on literature and case studies from previous research. Theoretically, the paper takes a generic value chain as a starting point together with the recent ISO standard on sourcing process and a business model framework. A conceptual framework is developed and typical sourcing strategies and business models for FM are investigated. Archetypical value chains are established by a combination of sourcing strategies and business models.

Findings
The paper identifies eight archetypes of FM value chains divided in three groups according to whether the core business organisation occupies rented facilities and owned facilities or has facilities operation as a core business like serviced office providers, etc.

Practical implications
The results can be used on a general level by everybody who need to get an overview and understanding of the complex structure of the FM sector. Furthermore, the results can help all parties involved in the FM value chain to get a clearer understanding of their position in the chain and help them develop their sourcing strategies and/or business model, depending on their type of organisation.

Originality/value
While there is a huge amount of literature on sourcing in FM, there has only been limited research on business models and value chains in FM. The paper is original in combining an investigation of sourcing strategies, business models and value chains in FM.
Systematic study on key factors influencing establish automobile electronics industry clustering
Authors: Wei Nai-Chieh , Hsu Hsiang-Chen , Chao I-Ming , Yang Ai-Ling 
Published: November 2019

Purpose
The purpose of this paper is to analyze the trends of global automobile industry toward energy saving and smart application. The automobile industry has its own special supply, safety and reliability system. It combined with the legislations that progressive safety driving leads to increase automobile electronic (AE) facilities and enlarges the proportion of the electronic control for vehicles. Based on matured communication and semiconductor industries in Taiwan, the government assists these hi-tech industries in entering the AE domain by clustering relevant enterprises with assembly supply chains. Flagship IC packaging and LCD panels enterprises are located in the Kaohsiung Industrial Park which possesses advantages for the clustering of AE industry.

Design/methodology/approach
In this paper, a systematic investigation on establishing AE industry cluster in Kaohsiung Industry Park (KIP) is presented. First, 42 electrical and electronics enterprises are site visited and interviewed for operation and management preferential demands. Second, problems encountered in enterprises’ operation are identified through expert weight method. Third, the main factors involved in the operational management are then analyzed based on five flagship enterprises in KIP. The integration of industrial–university–government resources and assistance is demonstrated. Finally, the establishment of an innovative talents cultivation model is illustrated.

Findings
The developed systematic talent program is proposed to resolve the urgent demands in recruiting talents, operational costs and new products development.

Originality/value
This paper satisfies needs of research enterprise management. The establishment of an innovative talents cultivation model is illustrated.
Rapid Appraisal for Agricultural Land Utilization in the erosion and landslide vulnerable mountainous areas of Kulonprogo Regency, Indonesia
Authors: Juhadi Juhadi , Banowati Eva , Sanjoto Tjaturahono Budi , Nugraha Satya Budi 
Published: November 2019

Purpose
The purpose of this paper is to assess the land utilization in the erosion and landslide vulnerable mountainous region using the Rapid Appraisal for Agricultural Land Utilization (RApALU) model.

Design/methodology/approach
A multidimensional RApALU model is used for sustainable agriculture land utilization.

Findings
Ecological dimension was less sustainable, whereas socio-economic, socio-cultural, and eco-technological dimensions were comparatively more sustainable. It was found from the analysis that 9 out of 21 attributes have sensitive effect on the sustainability index and status.

Practical implications
One of the implications of this research is that this model could be used to quickly measure the arrangement of an area that is experiencing environmental problems so that the land use planning process could be done more effectively and efficiently. The parameters used in each variable could be chosen by the researchers themselves according to location. As far as known by the researcher, the methods used have not been well integrated, they are still separated, for example, only physical problems, and social problems have not been measured properly. This model is not perfect yet, and it could be developed further because environmental problems are very complex and could be different from one location to another.

Originality/value
RApALU analysis can be used as preliminary analysis to comprehend general and overall description on the status of the sustainability index of land utilization for agriculture in hilly mountainous regions. The study confirmed that RApALU analysis can help determine the status of the sustainability of land utilization in intricate areas. This technique was able to comprehensively identify important factors affecting sustainability status of various dimensions.
Human resource management challenges facing franchise businesses
Authors: Zolfagharian Mohammadali , Naderi Iman 
Published: November 2019

PurposeThe purpose of this paper is to extend the current understanding of human resource management (HRM) challenges facing franchise businesses.Design/methodology/approachA qualitative investigation, including eight in-depth interviews and 66 semi-structured interviews with various franchise stakeholders as well as 42 participant observations, was conducted in North America to answer the research questions.FindingsSix major conclusions emerged from the conceptual and empirical work. The findings, for instance, reveal that human resources in franchise businesses lacks in motivation and skills, and franchisees’ distance from the ideal mix of autonomy and risk-aversion determines psychological and financial distress in the system.Practical implicationsThe findings suggest that when firms choose the franchising path as a means of leapfrogging resource constraints, they will experience new and more challenging HRM complications for several reasons. Therefore, decision-makers at both franchisor and franchisee firms need to address these new HRM challenges proactively by recognizing their possibility and emergence and by engaging in cooperative learning with one another.Originality/valueWhile HRM practices can “make or break” franchise systems, some important research questions still remain unanswered in this context. In an attempt to narrow this gap, and using a qualitative approach, this work identifies and classifies the key HRM challenges facing the franchise industry. Based on the finding, a conceptual model is proposed and discussed.
What happens during flight to safety: Evidence from public and private real estate markets
Authors: Boudry Walter I. , Connolly Robert A. , Steiner Eva 
Published: November 2019

AbstractLittle is known about the performance of public and private real estate investments during and after flight to safety (FTS). We document that public real estate securities offer a partial hedge during FTS events. In addition, periods with multiple FTS events forecast slower economic growth, resulting in weaker long‐run investment performance of real estate assets. Following FTS, the probability of a drop in real estate cash flows increases by up to 20%, with quarterly cash flow growth rates declining by as much as 27%. Private market real estate price appreciation over the four quarters following FTS is nearly wiped out.
Application of targeted exome and whole‐exome sequencing for Chinese families with Stargardt disease
Authors: Dan Handong , Huang Xin , Xing Yiqiao , Shen Yin 
Published: November 2019

AbstractObjectiveThe aim of this study was to investigate pathogenic variants and molecular etiologies of Stargardt disease (STGD) in a cohort of Chinese families.Materials and MethodsA cohort of 12 unrelated STGD families diagnosed on the basis of clinical manifestations underwent analysis by targeted exome or whole‐exome sequencing. Bioinformatics analysis, Sanger sequencing, and cosegregation analysis of available family members were used to validate sequencing data and confirm the presence of disease‐causing genes.ResultsUsing targeted exome and whole‐exome sequencing, we found that eight families had disease‐causing variants in the ABCA4 gene, one family had only one heterozygous variant in the ABCA4 gene, and the remaining three families have not been identified with any disease‐causing variants for STGD. We identified 15 variants in the ABCA4 gene; of these, five variants have not been previously described for STGD.ConclusionThe findings in this study expand the data regarding the frequency and spectrum of variants in the ABCA4 gene, thus potentially enriching our understanding of the molecular basis of STGD. Moreover, they constitute clues for future STGD diagnosis and therapy.