bluuebunny/crossref_metadata_2025_split huggingface dataset:
Climatology of Medium-Scale Traveling Ionospheric Disturbances (MSTIDs) Observed with GPS Networks in the North African Region
Authors: Oluwadare Temitope Seun , Jakowski Norbert , Valladares Cesar E. , Akala Andrew Oke-Ovie , Abe Oladipo E. , Alizadeh Mahdi M. , Schuh Harald 
Published: June 2020

Abstract
We present for the first time the climatology of medium-scale traveling ionospheric disturbances (MSTIDs) by using Global Positioning System (GPS) receiver networks on geomagnetically quiet days (Kp ≤ 3) over the North African region during 2008-2016. The MSTIDs appear frequently as oscillating waves or wave-like structures in electron density induced by the passage of Atmospheric Gravity Waves (AGW) propagating through the neutral atmosphere and consequently, causing fluctuation in the ionospheric Total Electron Content (TEC). The TEC perturbations (dTEC) data are derived from dual frequency GPS-measurements. We have statistically analyzed the MSTIDs characteristics, occurrence rate, seasonal behavior as well as the interannual dependence. The results show a local and seasonal dependence of nighttime and daytime MSTIDs. The propagation direction is predominantly towards the South (equatorward), MSTIDs event period is (12 ≤ period ≤ 53 mins), and dominant amplitude (0.08 ≤ amp ≤ ~1.5 TECU), with a propagation velocity higher at daytime than nighttime. The amplitudes of the MSTIDs increase with solar activity. The local MSTIDs Spatio-temporal heat reveals variability in disturbance occurrence time, but seems to be dominant within the hours of (Northwest: 1200–1600 LT) and (Northeast: 1000–1400 LT) in December solstice during daytime, and around (NW: 2100–0200 LT) and (NE: 1900–0200 LT) in June solstice, but get extended to March equinox during solar maximum (2014) during the nighttime. The time series of MSTIDs regional distribution map is also generated. Atmospheric gravity waves (AGW) seems to be responsible for the daytime MSTIDs occurrence.
Prevalence and genotype distribution of HPV infection among 214,715 women from Southern China, 2012-2018: baseline measures prior to mass HPV vaccination
Authors: Luo Lipei , He Ping , liu qiaotu , jiang Yanghua , Zhang Yang-nan , Li Qingzhao , Li Qiu , Li Shengtao , Yang Fan , Ling Hua , Dai Xingui , Li Zhongyu , Chen Hongliang 
Published: June 2020

Abstract
Background: The epidemiology on the human papillomavirus (HPV) among females in Southern China is not well-established. Baseline data on the prevalence of HPV infection in China prior to mass prophylactic HPV vaccination would be useful. Thus, this study aimed to determine the type-specific HPV prevalence and distribution among females from Southern China prior to mass HPV vaccination. Methods: A retrospective cross-sectional study employing 214,715 women attending ChenZhou NO.1 People’s Hospital for cervical screening during 2012-2018 was conducted prior to widespread HPV vaccination. HPV genotype was detected using nucleic acid molecular diversion hybridization tests. The overall prevalence, age-specific prevalence, type distribution, and annual trend were analyzed. Results: The overall HPV prevalence was 18.71% (95% confidence interval [CI], 18.55%-18.88%) among Southern China females. During 2012–2018, the prevalence of HPV infection showed a downward tendency, from 21.63% (95% CI, 21.07%-22.20%) in 2012 to 18.75% (95% CI, 18.35%-19.16%) in 2018. Age-specific HPV distribution displayed a peak at young women aged less than 21 years (33.11%, 95% CI, 31.13%-35.15%), 20.07% (95% CI, 19.70%-20.44%) among women aged 21-30 years, 17.29% (95% CI, 17.01%-17.57%) among women aged 31-40 years, 17.23% (95% CI, 16.95%-17.51%) among women aged 41-50 years, 21.65% (95% CI, 21.11%-22.20%) among women aged 51-60 years, and 25.95% (95% CI, 24.86%-27.07%) among women aged over 60 years. Of the 21 subtypes identified, the most three prevalent high-risk HPV (HR-HPV) genotypes were HPV52 (5.12%; 95% CI, 21.11%-22.20%), -16 (2.96%; 95% CI, 2.89%-3.03%), and -58 (2.51%; 95% CI, 2.44%-2.58%); the predominant low-risk HPV (LR-HPV) genotypes were HPV81 (1.86%; 95%CI, 1.80%-1.92%) and -6 (0.69%; 95% CI, 0.66%-0.73%) respectively. Incidence of HR-HPV only, LR-HPV only and mixed LR- and HR-HPV were 15.17%, 2.07% and 1.47% respectively. Besides, single HPV infection accounted for 77.30% of all positive cases in this study.Conclusions: This study highlights 1) a high prevalence of HPV infection among females with a decreasing tendency towards 2012-2018, especially for young women under the age of 21 prior to mass HPV vaccination; 2) HPV52, -16 and -58 were the predominant HPV genotypes, suggesting potential use of HPV vaccine covering these HPV genotypes in Southern China.
Effects on applying Micro-Film Case-Based Learning model in pediatrics education
Authors: Pan Yuan , Chen Xiuqi , Wei Qiuwen , Zhao Jinmin , Chen Xun 
Published: June 2020

Abstract
Background: In view of the harsh reality Chinese paediatricians face, the challenge of paediatric education is about instilling not only knowledge and clinical skills but also resilience and beliefs. The aim of the study is to explore a more effective method than the traditional lecture-based learning (LBL) model for optimizing educational outcomes by establishing an innovative, comprehensive, case-based learning (CBL) model combined with the micro-film technique (MF+CBL). This approach has four important components: interests (attraction), knowledge application, competency, and scenario coping skills. Methods: Experimental research was conducted via a controlled parallel group study. The total sample of 104 senior-year students (Chinese) majoring in clinical medicine was randomly divided into two groups. The experimental group was exposed to the MF+CBL model and the control group to the LBL model. Overall, the results were assessed after an 8-week course via a student self-assessment questionnaire, a satisfaction survey and the final examination.Results: The experimental group generally performed better than the control group on the student self-assessment (P＜0.05), satisfaction survey (P＜0.05), and final examination (80.02±3.77 vs 73.65±3.69, P = 0.000). The open question at the end of the questionnaire revealed that a small number of students did not favour the MF+CBL model due to its time- and energy-consuming features.Conclusions: Compared with LBL, the MF+CBL model was an innovative teaching method that promoted more comprehensive quality development. It represents an alternative model for optimizing the capacity of future paediatric doctors.
Clinical application of chromosomal microarray analysis for fetuses with craniofacial malformations
Authors: Xu Chenyang , Xiang Yanbao , Xu Xueqin , Zhou Lili , Li Huanzheng , Dong Xueqin , Tang Shaohua 
Published: June 2020

Abstract
Background: The potential correlations between chromosomal abnormalities and craniofacial malformations (CFMs) remain a challenge in prenatal diagnosis. This study aimed to evaluate 118 fetuses with CFMs by applying chromosomal microarray analysis (CMA) and G-banded chromosome analysis. Results: Of the 118 cases in this study, 39.8% were isolated CFMs (47/118) whereas 60.2% were non-isolated CFMs (71/118). The detection rate of chromosomal abnormalities in non-isolated CFM fetuses was significantly higher than that in isolated CFM fetuses (26/71 vs. 7/47, p = 0.01). Compared to the 16 fetuses (16/104; 15.4%) with pathogenic chromosomal abnormalities detected by karyotype analysis, CMA identified a total of 33 fetuses (33/118; 28.0%) with clinically significant findings. These 33 fetuses included cases with aneuploidy abnormalities (14/118; 11.9%), microdeletion/microduplication syndromes (9/118; 7.6%), and other pathogenic copy number variations (CNVs) only (10/118; 8.5%).We further explored the CNV/phenotype correlation and found a series of clear or suspected dosage-sensitive CFM genes including TBX1, MAPK1, PCYT1A, DLG1, LHX1, SHH, SF3B4, FOXC1, ZIC2, CREBBP, SNRPB, and CSNK2A1.Conclusion: These findings enrich our understanding of the potential causative CNVs and genes in CFMs. Identification of the genetic basis of CFMs contributes to our understanding of their pathogenesis and allows detailed genetic counselling.
Downregulation of Dickkopf-1 Augments the Therapeutic Effects of Endometrial Regenerative Cells on Experimental Colitis
Authors: Yu Dingding , Zhao Yiming , Hu Yonghao , Kong Dejun , Jin Wang , Qin Yafei , Zhang Baoren , Li Xiang , Hao Jingpeng , Wang Hongda , Li Guangming , Wang Hao 
Published: June 2020

Abstract
Background

We have demonstrated that endometrial regenerative cells (ERCs) are mesenchymal-like stromal cells and can attenuate experimental colitis, however, its underlying mechanism needs further investigation. Dickkopf-1 (DKK1), a glucoprotein secreted by mesenchymal stromal cells (MSCs), is a classical inhibitor of Wnt/β-catenin pathway which is closely associated with the development of colitis. Therefore, the objective of this study was to investigate whether ERCs could also secret DKK1, and whether the downregulation of DKK1 (DKK1 low -ERCs) would enhance the therapeutic effects of ERCs in attenuation of experimental colitis.

Methods

BALB/c mice were given 3% dextran sodium sulfate (DSS) for 7 consecutive days and free tap water for 3 days sequentially to induce experimental colitis. Unmodified ERCs, IL-1β-treated ERCs (DKK1 low -ERCs) and glucocorticoid-treated ERCs (DKK1 high -ERCs) were injected (1 million/mouse/day, i.v. ) on day 2, 5 and 8 respectively. Colonic and splenic samples were harvested on day 10 after DSS-induction.

Results

It was found that DKK1 low -ERC treatment markedly attenuated colonic damage, body weight loss and colon-length shortening in colitis mice. Compared with other treatments, cell populations of CD4 + IL-4 + Th2, CD4 + CD25 + FOXP3 + Treg, and CD68 + CD206 + macrophages in spleens were also significantly upregulated in DKK1 low -ERC group ( p < 0.05). In addition, lower expression of pro-inflammatory (TNF-α and IFN-γ), but higher levels of anti-inflammatory cytokines (IL-4 and IL-10) and β-catenin were detected in colons in DKK1 low -ERC group ( p < 0.01 vs. other groups).

Conclusions

DKK1 low -ERCs display augmented immunoregulatory ability and therapeutic effects in DSS-induced colitis.
Clinical features and outcome of fecal retention in infancy
Authors: Ren Lu , Geng Lan-Lan , Wang hong-li , Gong si-tang , Xie Jing , Xiong Li-Ya , Chen Pei-Yu 
Published: June 2020

Abstract
1) Background: To understand the clinical features and outcome of fecal retention in infancy(FRI), so as to guide the focus and intervention methods of such infants.2) Methods: The electronic medical record system(EMRS)was used to collect and screen out cases diagnosed as fecal retention from June 2018 to June 2019 in outpatient clinic of our hospital. The age, feeding method, frequency and traits of stool, and accompanying symptoms were recorded. The changes of clinical symptoms, medical examinations and drug treatment by the age of 1 year were investigated by means of electronic medical record review and telephone follow-up.3) Results: A total of 286 infants were enrolled, 7 were lost to follow-up, and 279 were effectively followed up. There were 157 males and 129 females, with an age of 3.6 ± 1.5 months. The average stool frequency was 5.9 ± 1.8 days. 63.3% of the infants were breast-fed, 16.8% were formula-fed, and the rest were mixed-fed,all without supplementary food. 9.1% of the infants showed corresponding gastrointestinal symptoms, such as bloating, increased crying, decreased milk intake, and laborious defecation. 87.1% of the infants received medical treatment, including glycerin enema, probiotics, and Chinese herbal preparations, with an effective treatment rate of 7.8%. 38.7% of infants have undergone medical examination, including abdominal ultrasound, X-ray film / barium enema, blood test, etc, the positive rate is 14.8%. The duration of fecal retention in 53% of infants was ≤ 2 months, 22.6% between 2–3 months, 24.4% ≥ 3 months, with an average of 2.6 ± 1.1 months. At the age of followed up to 1 year, 16.8% of infants developed functional constipation(FC). Compared with other infants with normal defecation, there was no significant difference in age, frequency of stool, the proportion of breast milk feeding and receiving treatment, and there was a significant difference in the duration of fecal retention. The duration of FC group was longer than normal defecation group by which was 3.49 ± 0.83 months.4) Conclusions: Infants with fecal retention are more likely to develop FC at age 1 than general population, and may be positively related to the duration of fecal retention.
Predictors of Patient Satisfaction; Quality Dimensions and Demographic Characteristics
Authors: Farrokhi Pouria , Hashjin Asgar Aghaei , Bagherzadeh Rafat , Zarei Ehsan 
Published: June 2020

Abstract
Background: The healthcare industry is increasingly growing in a competitive atmosphere. One of the essential key issues for the survival of healthcare organizations is patient satisfaction. This study aimed to investigate the impact of health service quality and demographic characteristics on patient satisfaction with outpatient departments at teaching hospitals affiliated with Tehran University of Medical Sciences in Iran.Method: This cross-sectional study was conducted in 2018. A sample of 400 patientsreferring to outpatient departments at teaching hospitalswasrandomly selected. A valid and reliable questionnaire was used to collect data which were then analyzed by using SPSS 23. Results: The mean scores of service quality and patient satisfaction were 3.73 ± 0.51 and 3.61 ± 0.97 out of 5, respectively. Moreover, patients’ demographic characteristics; age, marital status, residence area, and service quality dimensions; admission process, physician consultation, service costs, accessibility, and appointment were identified as the most effectivefactors on patient satisfaction. Conclusion: In order to increase patient satisfaction, physicians and reception staff are advised to provide patients with useful information and cost-effective services. Nonetheless,it seems necessary for teaching hospitalsto establish plans which facilitate payment, appointment, and examination process.
Arthroscopic Cartilage Regeneration Facilitating Procedure: A Decompressing Arthroplasty for Knee Osteoarthritis
Authors: Lyu Shaw-Ruey , Hung Jung-pin , Hsu Chia-Chen , Chen Yu-Ruei , Lin Chih-Wen 
Published: June 2020

Abstract
Background The effectiveness of arthroscopic treatment for knee osteoarthritis (OA) has been subject to debate. This study presents an innovative concept of arthroscopic management for knee OA and investigates its clinical outcome.Methods An arthroscopic cartilage regeneration facilitating procedure (ACRFP) to eliminate medial abrasion phenomenon and decompress the patello-femoral joints was performed on 693 knees of 411 patients with knee OA, mean age 60 years (34-90). The Knee Society score (KSS) and the knee injury and osteoarthritis outcome score (KOOS) were used for subjective outcome study. Roentgenographic changes for all cases and magnetic resonance imaging (MRI) variations for twenty randomly selected cases were evaluated for objective outcomes. Results There were 634 knees in 369 patients (93.7%) available with more than 3 years of follow-ups (mean 40 months, SD 9). The overall subjective satisfactory rate was 91.1%. The KSS and all subscales of the KOOS improved statistically. Reversal of the degeneration process of cartilage was observed in 80.1% of the whole series (radiographic outcome) and in 72.2% of the 18 randomly selected cases (one-year MRI outcome study). Gender and OA severity were related to subjective outcomes. Age, body mass index (BMI), pre-operative hyaluronic acid injection, OA severity, and the type and severity of the medial plica are important predictors of radiographic outcomes. An analysis of failed cases reaffirmed the need for early ACRFP and skillful post-operative care. Conclusion ACRFP is an effective treatment for knee OA. If performed in time, it can satisfy most patients and might modify their degeneration process.
Lipidomic Profiling Reveals Distinct Differences in Plasma Lipid Composition in Overweight or Obesity Adolescence Student
Authors: Yang Longyan , Yin Ruili , Wang Xiaojing , Yu Ke 
Published: June 2020

Abstract
Introduction：The relationship between dyslipidemia and obesity has been widely reported, but the global lipid profiles associated with the development of obesity remain to be clarified, especially in the East Asia teenage population. Methods：Mass spectrometry coupled with liquid chromatography was applied to detect the global lipidome in the fasting plasma from 90 Chinese adolescence, including 30 obesity adolescents, 30 overweight adolescents, and 30 adolescents with normal body mass index (BMI). All participants were performed anthropometric measurement by using InBody 770. Clinical biochemical indicators were measured by Cobas Elecsys 601.Results：Both qualitative and quantitative analyses revealed a gradual change in plasma lipid features with obesity students exhibiting characteristics close to overweight students, but they differed significantly from students with normal. The levels of triglyceride (TG), 18-Hydroxycortisol, Isohumulinone A, and 11-Dihydro-12-norneoquassin were up-regulated in obesity group, while phosphatidylcholine (PC), phosphatidylethanolamine (PE), LysoPC, LysoPE, and Phosphatidylinositol (PI) were significantly down-regulated in obesity group than in control and overweight individuals. Then conducted venn diagram and selected 8 significant metabolites from the 3 paired comparisons. Most of the selected features significantly correlated with anthropometric measurement.Conclusions: The altered plasma lipidome in Chinese obesity and overweight students suggests that lipid features may play important role in the pathogenesis of obesity and that such features may provide a basis for evaluating risk and monitoring obesity development.
Effects of alendronate in bone repair: A study in rat femur fractures treated with plates and screws
Authors: Tiboni Fernanda , Weiss Suyany Gabriely , Gerber Jennifer Tsi , Michels Arieli Carini , Rosa Thais Grupp da , Casagrande Thaís Costa , Deliberador Tatiana Miranda , Giovanini Allan Fernando , Johann Aline Cristina , Scariot Rafaela 
Published: June 2020

Abstract
Background

Alendronate (ALN) has direct action on bone metabolism, increasing osteogenesis and decreasing bone resorption. The study rated the effect of ALN on femoral fracture repair and the effect of different doses of the drug on the liver and kidneys.
Methods

Wistar rats were divided into groups: A1 (ALN 1 mg/kg), A2 (ALN 3 mg/kg), and C (saline solution). The drug/solution was applied intraperitoneally three times a week after femoral fracture until euthanasia 45 days later.
Results

Liver analysis from group A1 presented normal histological aspects, while hepatic steatosis was observed in group A2. In groups A1 and A2, kidneys showed amylocymile like cell degeneration. In femur bone callus, no difference was observed in collagens I and III or in number of trabeculae (p ≥ 0.05). Immunohistochemical evaluation showed positivity for the Transforming Grow Factor β-1 (TGFβ-1) marker in the control group, in spinal area and in small chondrocytes, but negativity for hypertrophy. In A1, an extensive area of cartilaginous expansion was observed, with positive hypertrophic TGFβ-1 cartilage, even in areas with bone matrix. A low positivity was observed in the medullar area, in contrast to the control. Group A2 presented a high number of chondroid matrices and a moderate number of TGFβ-1 cells, with little positivity in medullary area.
Conclusions

A dosage of ALN 1 mg/kg promotes cellular differentiation activity in the bone callus region, with mild damage in the liver and kidneys. A dosage of ALN 3 mg/kg became toxic without positive alterations in cell differentiation.
Tissue-nonspecific Alkaline Phosphatase Blockade Ameliorates Behavioural Deficits and Life-expectancy on Tauopathies
Authors: Sebastián-Serrano Álvaro , Merchán-Rubira Jesús , Di-Lauro Caterina , Bianchi Carolina , Narisawa Sonoko , Millan Jose Luis , Avila Jesus , Hernandez Felix , Diaz-hernandez Miguel 
Published: June 2020

Abstract
The authors have withdrawn this preprint due to erroneous posting.
Transjugular Intrahepatic Portosystemic Shunt for Hepatic Sinusoidal Obstruction Syndrome Associated with Consumption of Gynura Segetum
Authors: Zhang Lijie , Li Qing , Makamure Joyman , Zhao Dan , Liu Ziyi , Zheng Chuansheng , Liang Bin 
Published: June 2020

Abstract
Background: To evaluate the efficacy and safety of transjugular intrahepatic portosystemic shunt (TIPS) on hepatic sinusoidal obstruction syndrome (HSOS) associated with consumption of Gynura segetum (GS).Methods: We retrospectively reviewed 9 consecutive patients with GS-related HSOS who were refractory to supportive treatment and underwent TIPS at our institution between January 2014 and September 2019. The patients were evaluated for safety and efficacy, including TIPS complications and changes in portosystemic pressure gradient (PPG), ascites, total bilirubin, liver size and portal vein diameter.Results: TIPS procedures were performed successfully in the 9 patients, without any TIPS technique-related complications. The PPG was improved by TIPS in all patients (mean PPG before TIPS, 30.4 ± 5.2 vs. 13.0 ± 4.1 mm Hg post-TIPS, P=0.008). Except for one patient who was lost to follow-up, the remaining 8 patients survived with a median follow-up period of 12 months (range, 5-39 months). Although the level of total bilirubin was significantly increased 5-7 days after TIPS compared with that before the procedure (3.57 ± 1.58 vs. 4.82 ± 2.06 mg/dl, P=0.017), it returned to baseline level at 1-month follow-up (3.53 ± 2.72 vs. 4.82 ± 2.06 mg/dl, P=0.401). The patients experienced complete resolution or obvious reduction of ascites (P<0.001), significant reduction of liver size (16.7 ± 2.2 vs. 13.7 ± 1.7 cm, P=0.018), and significant enlargement of the portal trunk (10.7 ± 2.5 vs. 13.4 ± 2.4 mm, P=0.017) after TIPS compared to the pre-TIPS state.Conclusion: TIPS may offer a potentially useful treatment for the GS-related HSOS.