bluuebunny/crossref_metadata_2025_split huggingface dataset:
Farm staff wrong movements affects PRRSv prevalence and viremia
Authors: Díaz Inmaculada , Jiménez-Martín Manuel , Rodríguez María , Escudero Cristina , Aparicio María , Morales Joaquin , Piñeiro Carlos 
Published: January 2020

Abstract
Background Biosecurity is known as the implementation of measures to reduce the risk of introduction (external biosecurity) and spread (internal biosecurity) of disease agents. One of the most common diseases in the porcine industry is the porcine reproductive and respiratory syndrome (PRRS), which has a huge negative impact on the well-being of the animals and consequently, on their productivity. Nonetheless, most of the biosecurity evaluation tools are based on scored systems. A new digital biosecurity system was designed to help control PRRS virus (PRRSv) infection status throughout an objective tool for the evaluation of internal biosecurity based on a system of control of the flow of internal movement of personnel in commercial farms. Movements, routes and health data were combined to classify the staff movements into three categories including “Risky” (From PCR(+) to PCR(-) barns), “Unsafe” (between PCR(+) barns) and “Safe” (From PCR(-)). Therefore, the main aims of the present work were to evaluate the efficacy of this new tool, its relationship with PRRSv incidence as well as to demonstrate the importance of biosecurity education to help farm workers to adopt safer daily practices. Results The observed results showed an overall smaller number of monthly movements (p < 0.05) and a significant increase in the Safe movements percentage (p < 0.05), concomitant with a decrease in the Risky movements percentage (p < 0.05) after the training session. In regards the relationship between staff movements and PRRSv presence, neither the percentage nor the total amount of both Safe and Unsafe movements were significantly different between the PCR(+) and PCR(-) groups of PRRSv status (p > 0.05). Nonetheless, both the total number and the percentage of Risky movements were significantly lower in the PCR(-) group (p < 0.05) compared with PCR(+) group. These results show a clear relationship between the total amount of Risky movements and the probability of a PRRSv outbreak in the farms. Conclusions Our results support the notion that staff movement patterns within the different farm areas are a major factor in its internal biosecurity. The new tool described in the current work showed a significant relationship between staff movements and the probability of PRRSv outbreak and demonstrate the importance of biosecurity training to help farm workers adopt safer daily practices.
Effects of EHP-101 on inflammation and remyelination in murine models of Multiple Sclerosis
Authors: Navarrete Carmen , García-Martin Adela , Garrido-Rodriguez Martín , Mestre Leyre , Feliu Ana , Guaza Carmen , Calzado Marco A , Muñoz Eduardo 
Published: January 2020

Abstract
Background: Multiple Sclerosis (MS) is characterized by a combination of inflammatory and neurodegenerative processes in the spinal cord and the brain. Natural and synthetic cannabinoids such as VCE-004.8 have been studied in preclinical models of MS and represent promising candidates for drug development. VCE-004.8 is a multitarget synthetic cannabidiol (CBD) derivative acting as a dual Peroxisome proliferator-activated receptor-gamma/ Cannabinoid receptor type 2 (PPAR γ /CB 2) ligand agonist that also activates the Hypoxia-inducible factor (HIF) pathway. EHP-101 is an oral lipidic formulation of VCE-004.8 that has shown efficacy in several preclinical models of autoimmune, inflammatory, fibrotic and neurodegenerative diseases.
Methods: The efficacy of EHP-101 in vivo was evaluated in two murine models of MS, the experimental autoimmune encephalomyelitis (EAE) and cuprizone-induced demyelination models. In EAE, transcriptomic analysis was performed by RNA-Seq and qPCR, and inflammatory and myelination markers were detected by immunohistochemistry (IHC) and confocal microscopy in both models of MS.
Results: EHP-101 alleviated clinical symptomatology in EAE and transcriptomic analysis demonstrated that EHP-101 prevented the expression of many inflammatory genes closely associated with MS pathophysiology in the spinal cord. EHP-101 normalized the expression of several genes associated with oligodendrocyte function such as Teneurin 4 (Tenm4) and Gap junction gamma-3 (Gjc3) that were downregulated in EAE. EHP-101 treatment prevented microglia activation and demyelination in both the spinal cord and the brain. Moreover, EAE was associated with a loss in the expression of Oligodendrocyte transcription factor 2 (Olig2) in the corpus callosum, a marker for oligodendrocyte differentiation, which was restored by EHP-101 treatment. In addition, EHP-101 enhanced the expression of glutathione S-transferase pi (GSTpi), a marker for mature oligodendrocytes in the brain. We also found that a diet containing 0.2 % cuprizone for six weeks induced a clear loss of myelin in the brain measured by Cryomyelin staining and Myelin basic protein (MBP) expression. Moreover, EHP-101 also prevented cuprizone-induced microglial activation, astrogliosis and reduced axonal damage.
Conclusions: Our results provide evidence that EHP-101 showed potent anti-inflammatory activity, prevented demyelination and enhanced remyelination. Therefore, EHP-101 represents a promising drug candidate for the potential treatment of different forms of MS.
Age-dependent involvement of gut mast cells and histamine in post-stroke inflammation
Authors: Blasco Maria Pilar , Chauhan Anjali , Honarpisheh Pedram , Ahnstedt Hilda , d’Aigle John , Ganesan Arunkumar , Ayyaswamy Sriram , Blixt Frank , Venable Susan , Major Angela , Durgan David , Haag Anthony , Kofler Julia , Bryan Robert , McCullough Louise D , Ganesh Bhanu Priya 
Published: January 2020

Abstract
Background Risk of stroke-related morbidity and mortality increases significantly with age. Aging is associated with chronic, low-grade inflammation, which is thought to contribute to the poorer outcomes after stroke seen in the elderly. Histamine (HA) is a major molecular mediator of inflammation and mast cells residing in the gut are a primary source of histamine. Methods Stroke was induced in male C57BL/6J mice at 3 months (young) and 20 months (aged) of age. Role of histamine after stroke was examined using young (Yg) and aged (Ag) mice, mice underwent MCAO surgery and were euthanized at 6h, 24h and 7 days post-ischemia; sham mice received the same surgery but no MCAO. In this work, we evaluated whether worsened outcomes after experimental stroke in aged mice was associated with age-related changes in mast cells, histamine levels, and histamine receptor expression in the gut, brain, and plasma. Results We found increased numbers of mast cells in the gut and the brain with aging. Using the middle cerebral artery occlusion (MCAO) model of ischemic stroke, we demonstrate that stroke leads to increased numbers of mast cells and histamine receptors in the gut. These gut-centric changes are associated with elevated levels of HA and other pro-inflammatory cytokines including IL-6, G-CSF, TNF-α, and IFN-γ in the peripheral circulation. Our data also shows that post-stroke gut inflammation led to a significant reduction of mucin-producing goblet cells and a loss of gut barrier integrity. Lastly, gut inflammation after stroke is associated with changes in the composition of the gut microbiota as early as 24 hours post-stroke. Conclusion An important theme emerging from our results is that acute inflammatory events following ischemic insults in the brain persist longer in the aged mice when compared to younger animals. Taken together, our findings implicate mast cell activation and histamine signaling as a part of peripheral inflammatory response after ischemic stroke, which are profound in aged animals. Interfering with histamine signaling orally might provide translational value to improve stroke outcome.
Predictive factor for bone cement implantation syndrome in osteoporotic femoral neck fracture undergoing cemented hip arthroplasty: a retrospective review of 142 cases
Authors: Chulsomlee Kulapat , Prukviwat Sasichol , Kulachote Noratep , Sirisreetreeux Norachart , Thamyongkit Sorawut , Tuntiyatorn Panithan , Vasaruchapong Satetha , Sa-ngasoongsong Paphon 
Published: January 2020

Abstract
Background Bone cement implantation syndrome (BCIS) is a serious and potentially fatal complication in cemented hip arthroplasty (CHA). Although previous studies have identified patients at risk for BCIS, few studies have reported on the association between BCIS and perioperative surgical factors in patients with osteoporotic femoral neck fracture (OFNF) undergoing CHA. This study aimed to identify the prevalence of BCIS and its related outcomes, correlating between BCIS and perioperative surgical factors in these patients. Materials and methods A single-centred, retrospective study was conducted between 2015 and 2018 with patients who sustained OFNF and underwent CHA. Perioperative data and BCIS-related outcomes were reviewed using electronic database and telephone interview. BCIS grading was classified according to the Donaldson classification. All patients were allocated into 2 groups: Group A (BCIS grade 0 or 1: BCIS-gr0/1) and Group B (BCIS grade 2 or 3: BCIS-gr2/3). Multiple logistic regression analysis was used to identify predictive factors for BCIS-gr2/3. Results A total of 142 OFNF patients were included. The prevalence of BCIS-gr2/3 was 6.4% (n=9). Group B showed a significantly higher major complication rate than Group A (44.4% vs. 8.3%, respectively, p<0.01). The in-hospital and 1-year mortality rates in Group B were also higher than in Group A, but non-significantly different ( p =0.12 and p =0.32, respectively). Multivariate analysis showed that preinjury non-ambulatory status (odds ratio [OR] 11.83; 95% confidence interval [CI], 1.06–131.8, p =0.04), arrhythmia (OR 5.65; 95% CI, 1.13–28.93, p =0.04) and shape-closed femoral stem type (OR 9.78; 95% CI, 1.65–57.80, p =0.01) were the significant independent predictors for BCIS-gr2/3 in these patients. Conclusion BCIS in OFNF patients undergoing CHA is common and associates with a high major perioperative complication rate. The patients at risk for BCIS-gr2/3 are those with poor preinjury ambulatory status, those with an underlying cardiac arrhythmia, and those whose CHA procedures use a shape-closed femoral stem design.
Analysis of global spine sagittal parameters in cervical spondylotic patients and asymptomatic subjects
Authors: Zhu Liang , Ma Xiaojun , Shen Qiang , Sun Bao , Fu Qiang 
Published: January 2020

Abstract
Background: Degenerative changes associated with cervical spondylotic can result in a change of normal sagittal alignment, and this may be the initial change of kyphosis and sagittal imbalance. Few studies have analyzed the correlations between the cervical spine lordosis and global spine balance in patients with cervical spondylotic. This study is applied to investigate the characteristics and relationships of cervical and global sagittal parameters in normal adults and cervical spondylotic patients. Methods: We reviewed 46 asymptomatic control subjects (normal group, NG) and 48 cervical spondylotic patients (cervical spondylotic group, CSG), who had both cervical MRI and global radiographs obtained together, between January 2016 and September 2018. Data includes C1-2 angle, C2–7 lordosis (CL), T1 slope (T1S), thoracic inlet angle (TIA), C2–7 sagittal vertical axis (CSVA), sagittal vertical axis(SVA), thoracic-kyphosis(TK), thoracic-lumbar lordosis(TL), lumbar lordosis (LL), pelvic incidence (PI), pelvic tilt (PT) and sacral slope (SS). The values were presented as the mean ± standard deviation. Student t-test and Pearson’s correlation coefﬁcient were used for statistical analysis. Probability values less than 0.05 were considered statistically signiﬁcant. Results: 1.Comparison of global sagittal parameters between the normal group and cervical spondylosis group. A total of 48 cervical spondylotic patients with an average age of 57.91±9.58 and 46 healthy people with an average age of 28.00±8.09 were recruited in our study. CL in the NG was significantly lower than CSG (P<0.05), while TK and TL were significantly lower than in the CSG(P< 0.01). However, a comparison of the NG and CSG yielded no significant differences in C1-2, TIA, TIS, LL, PT, PI, SS, CSVA, and SVA. 2.The relationship between cervical and global sagittal alignment. CL positively correlated with T1S (r=0.433) and TK (r=0.335) while negatively correlated with CSVA (r=-0.309) in cervical spondylosis group. TIA has positively correlated with T1S (r=0.376 in NG and r=0.416 in CSG) and no correlated with other parameters in both groups. Conclusions Cervical spondylosis causes changes in sagittal parameters of the cervical and thoracic spine but does not affect on lumbar and pelvic parameters. TIA is a relative constant parameter, not affected by cervical spondylotic.
Predictors associated with perioperative blood transfusion and intraoperative blood loss in thoracolumbar tuberculosis
Authors: Song Chengchao , Liu Chao , Wei Rongzhi , Zhang Qiuhua , Wu Feng , Wang Shengyu , Yan Jinglong , Wang yufu 
Published: January 2020

Abstract
Background During operation on thoracic and lumbar tuberculosis infection, patients can lose a significant amount of blood and receive a perioperative blood transfusion. However, the risk factors were not identified for increased intraoperative blood loss and perioperative blood transfusion. The aim of this retrospective study is to determine the predictors associated with perioperative blood transfusion and intraoperative blood loss in thoracolumbar tuberculosis. Methods From 2008 to 2018, 336 patients who met the inclusion criteria were enrolled in the study. The predictors of allogenic blood transfusion were identified in a univariate and multivariate logistic regression analysis. Univariate and multivariate linear regression was attempted to investigate the factors influencing intraoperative blood loss. Results Altogether, 336 adult patients with thoracic and lumbar tuberculosis were included in this study. The mean patient age was 49.6 ± 15.5 (range 14-85) years for those patients. Our data revealed a significant relationship between blood transfusions and female gender, BMI, vertebral collapse/Kyphosis and intraoperative blood loss. Multivariable linear regression analysis revealed that BMI, levels of instrumentation, surgical approach and operative time were independent factors influencing intraoperative blood loss. Conclusions This study identified some clinical predictors for perioperative blood transfusion and intraoperative blood loss in patients undergoing thoracic and lumbar tuberculosis surgery. These results may contribute to preoperative blood transfusion planning and minimize intra- or post-operative complications.
Pre-operative denosumab is associated with higher risk of local recurrence in giant cell tumor of bone: a systematic review and meta-analysis
Authors: Chen Xi , Li Hairui , Zhu Shibai , Wang Yiou , Qian Wenwei 
Published: January 2020

Abstract
Background In 2013, denosumab was introduced as peri-operative adjuvant treatment for giant cell tumor (GCT) of bone as it inhibits osteoclast activity. It is suggested that denosumab relives pain, facilitate curettage in lesions requiring resection initially. However, controversy remains whether denosumab increases the risk of local recurrence after surgery. Methods Medline, Embase and the Cochrane Library were comprehensively searched in June 2019 to identify studies investigating the clinical outcome of GCT of bone with and without peri-operative denosumab after surgery. Data were gathered and a meta-analysis was conducted. Result Ten studies with 1082 cases (169 in denosumab group, 913 in control group) were included. Overall, denosumab was associated with significantly higher risk of recurrence(P<0.02) and inferior 5 year recurrence free survival(P=0.000). Denosumab and curettage has a relatively higher risk of recurrence comparing to curettage alone(P=0.07). The risk of recurrence is not significantly increased if denosumab was administered both preoperatively and postoperatively(P=0.24). Conclusion Administration of denosumab is associated with increased risk of recurrence due to a variety of reasons, though it is proven effective in relieving pain, enabling curettage and improved functional outcome. Post-operative denosumab is recommended as it continuously suppress/eliminate residue tumor cells.
Application of preoperative assessment of pain induced by venous cannulation in predicting postoperative pain in patients under laparoscopic nephrectomy: a prospective study
Authors: Peng Fei , Li Yanshuang , Ai Yanqiu , Yang Jianjun , Wang Yanping 
Published: January 2020

Abstract
Backgroud: Postoperative pain is the most prominent concern among surgical patients. It has been reported that venous cannulation-induced pain can predict postoperative pain after laparoscopic cholecystectomy within 90 mins. Its potential in predicting postoperative pain in patients with patient-controlled intravenous analgesia (PCIA) is worth establishing. The purpose of this study was to investigate the application of VCP in predicting postoperative pain in patients with PCIA during the first 24 h after laparoscopic nephrectomy. Methods: 120 patients scheduled for laparoscopic nephrectomy. The nurse recorded the preoperative venous cannulation-induced pain score estimated by patients, and dichotomized the patients into VAS scores < 2.0 group or VAS scores ≥ 2.0 group . After general anesthesia and surgery, all the patients received the patient-controlled intravenous analgesia (PCIA) with sufentanil. The VAS scores at rest and on coughing at 2 h, 4 h, 8 h, 12 h, 24 h, the effective number of presses and the number of needed rescue analgesia within 24 h after surgery were recorded. Results: Venous cannulation-induced pain score was significantly correlated with postoperative pain intensity at rest (rs = 0.64) and during coughing (rs = 0.65), effective times of pressing (rs = 0.59), additional consumption of sufentanil (rs = 0.58). Patients with venous cannulation-induced pain intensity ≥ 2.0 VAS units reported higher levels of postoperative pain intensity at rest (P < 0.0005) and during coughing (P < 0.0005), needed more effective times of pressing (P < 0.0005) and additional consumption of sufentanil (P < 0.0005), and also needed more rescue analgesia (P = 0.01). The odds of risk for moderate or severe postoperative pain (OR 3.5, 95% CI 1.3-9.3) was significantly higher in patients with venous cannulation-induced pain intensity ≥ 2.0 VAS units compared to those < 2.0 VAS units. Conclusions: Preoperative venous cannulation-induced pain can be used to predict postoperative pain intensity in patients with PCIA during the first 24 h after laparoscopic nephrectomy. Trial registration: We registered this study in a Chinese Clinical Trial Registry (ChiCTR) center on July 6 2019 and received the registration number: ChiCTR1900024352. Key words: Venous cannulation, Pain, Postoperative pain, Pain prediction
Minimally invasive interventions for pulmonary nodules biopsy. Systematic review protocol.
Authors: Miotto Andre , Perfeito João Aléssio Juliano , Leite Rafael Pacheco , Latorraca Carolina de Oliveira Cruz , Riera Rachel 
Published: January 2020

Abstract
Background Lung cancer is the most common malignancy, causing more than 1.6 million deaths annually worldwide, including approximately 26.500 deaths in Brazil alone in 2015. The diagnosis of pulmonary nodules remains a challenge. Imaging tests are important for diagnostic suspicion and for estimating risk, but biopsy is necessary to confirm malignancy. Minimally invasive methods currently used include tomography-guided percutaneous biopsy (CTGB) and bronchoscopy transbronchial biopsy. The method of choice should have the best accuracy with the lowest possible complication rate. This systematic review was designed to map, critically evaluate and compare the effects (benefits and risks) of all lung nodule biopsy techniques. Methods We will conduct a systematic review accordantly to the Cochrane Handbook for Systematic Reviews of Interventions recommendations. Discussion This study aims to compare the diagnostic yield and the safety of different techniques used for pulmonary nodule biopsies. We will include randomized clinical trials comparing tomography-guided percutaneous biopsy, non-guided transbronchial biopsy, fluoroscopy-guided transbronchial biopsy, transbronchial biopsy guided by endobronchial ultrasound with radial probe and transbronchial biopsy guided by electromagnetic navigation. This study was approved by the research ethics committee of Universidade Federal de São Paulo (UNIFESP) with the number 1344040918. The results of the completed protocol will be presented at any appropriate conference by the authors.Systematic review registration The protocol for this review was prospectively registered in PROSPERO database (C RD42018092367).
Discovery, Identification, and Functional Characterization of Long Noncoding RNAs in Arachis hypogaea L.
Authors: Tian Haiying , Guo Feng , Zhang Zhimeng , Ding Hong , Meng Jingjing , Li Xinguo , Peng Zhenying , Wan Shubo 
Published: January 2020

Abstract
Background Long noncoding RNAs (lncRNAs), which are typically >200 nt in length, are involved in numerous biological processes. Studies on lncRNAs in the cultivated peanut (Arachis hypogaea L.) largely remain unknown. Results A genome-wide scan of the peanut (Arachis hypogaea L.) transcriptome identified 1,442 lncRNAs, which were encoded by loci distributed over every chromosome. Long intergenic noncoding RNAs accounted for 85.58% of these lncRNAs. Additionally, 189 lncRNAs were differentially abundant in the root, leaf, or seed. Generally, lncRNAs showed lower expression levels, tighter tissue-specific expression, and less splicing than mRNAs. The majority of the lncRNAs featured an exon/intron structure, and approximately 44.17% were alternatively spliced; this rate was slightly lower than the splicing rate of mRNA. Transcription at the start site event was the alternative splicing (AS) event with the highest frequency (28.05%) in peanut lncRNAs, whereas the occurrence rate (30.19%) of intron retention event was the highest in mRNAs. AS changed the target gene profiles of lncRNAs and increased the diversity and flexibility of lncRNAs, which may be important for lncRNAs to execute their functions. Additionally, a substantial number of the peanut AS isoforms generated from protein-encoding genes appeared to be noncoding because they were truncated transcripts; such isoforms can be legitimately regarded as a class of lncRNAs. The predicted target genes of the lncRNAs were involved in a wide range of biological processes. Furthermore, expression pattern of several selected lncRNAs and their target genes were examined under salt stress, results showed that all of them could respond to salt stress in different manners. Conclusions This study provided a resource of candidate lncRNAs and expression patterns across tissues, and whether these lncRNAs are functional will be further investigated in our subsequent experiments.