bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comparison of Six Approaches for Measuring Utility Values Among Patients With Locally Advanced Cervical Cancer: a Cross-sectional Study
Authors: Katanyoo Kanyarat , Thavorncharoensap Montarat , Chaikledkaew Usa , Riewpaiboon Arthon 
Published: December 2020

Abstract
Background: A variety of instruments to measure health utilities are available. Nevertheless, there was a lack of agreement between utility scores generated by the different instruments. To avoid difference in estimates of cost-effectiveness arising from the use of different instruments, it is essential to know whether these widely used instruments can be used interchangeable or not. While Euro-Quality of Life Five-Dimension (EQ-5D) is recommended as a preferred instrument for measuring health utilities there is no clear consensus on the preferred version of EQ-5D. Besides EQ-5D, time-trade-off (TTO), and visual analog scale (VAS) were commonly used instruments. This study aims to investigate agreement and performance of the following 6 utilities measures; EQ-5D-3L, EQ-5D-5L (cTTO Model), EQ-5D-5L (DCE Model), EQ-5D-5L (Hybrid model), TTO, and VAS among patients with locally advanced cervical cancer in Thailand. Methods: Face-to-face interview were conducted with 194 locally advanced cervical cancer patients. Comparison of median utility scores derived from the 6 approaches was made using Friedman test. The agreement of utility scores between each pairwise comparison was assessed by intraclass correlation coefficient (ICC) and Bland-Altman plot. Known-validity was determined by comparing two known groups with respect to status of treatment (i.e. treatment, disease free, and progression), and presences of symptoms (no symptoms, 1 symptom, and > 1 symptoms). Results: The mean (SD) utility values derived from the 6 approaches were, from low to high, 0.755±0.248 (EQ-5D-3L), 0.801±280 (TTO), 0.806±0.156 (VAS), 0.871±0.184 (cTTO model), 0.875±0.168 (Hybrid model), and 0.900±0.142 (DCE model). Intraclass correlation coefficient showed high agreement between EQ-5D-5L and EQ-5D-3L and very high agreement between all 3 models of EQ-5D-5L. Bland-Altman plots showed wide limit of agreement except the pairwise comparison between each model of the EQ-5D-5L; cTTO model and DCE model, cTTO model and Hybrid model, and DCE model and Hybrid. All 6 approaches can significantly differentiate treatment status and presence of symptoms.Conclusions: The commonly used utility measures, which are TTO, VAS, EQ-5D-3L and EQ-5D-5L cannot be used interchangeable among locally advanced cervical cancer patients. High agreement was found only between each pairwise comparison of the 3 value sets of EQ-5D-5L.
Synergistic Impact of Symptom Clusters on Health-related Quality of Life in Patients With Chronic Obstructive Pulmonary Disease: a Secondary Data Analysis
Authors: Fei Fei , Koffman Jonathan , Zhang Xiaohan , Gao Wei 
Published: December 2020

Abstract
Background: Clinical experiences and middle range Theory of Unpleasant Symptoms indicate that the existence of symptom clusters (SCs) may synergistically affect patients’ health-related quality of life (HRQoL). However, no studies have examined this issue. The aim of this study was to identify symptom clusters and quantify the synergistic impact of symptom clusters on health-related quality of life among patients with chronic obstructive pulmonary disease (COPD).Methods: Secondary data analysis of cross-sectional data collected via convenience sampling from patients with COPD in a Chinese university hospital. Assessments included modified MRC dyspnoea scale, Beck Depression Inventory, Beck Anxiety Inventory and Pittsburgh Sleep Quality Index. Health-related quality of life was assessed using the St George's Respiratory Questionnaire. Chi-squared tests were used to identify symptom clusters. Multiple linear regressions were used to examine associations between symptom clusters and health-related quality of life.Results: 106 COPD subjects were recruited. Three symptom clusters were identified in COPD patients and comprised of dyspnoea and depression (OR=2.69, 95%CI:1.19-6.02); anxiety and sleep (OR=2.72, 95%CI:1.20-6.15); depression and anxiety (OR=6.13, 95%CI:2.57-14.60). Two symptom clusters were identified in patients with severe-stage COPD, which were anxiety and sleep (OR=6.21, 95%CI:1.70-22.74), depression and anxiety (OR=5.33, 95%CI:1.64-17.40). All three symptom clusters (anxiety and sleep; dyspnoea and depression; depression and anxiety) were independently associated with health-related quality of life (β=14.56, 95%CI: 5.80-23.31; β =13.95, 95%CI:5.72-22.18; β=13.30, 95%CI:6.88-19.73).Conclusions: This is the first study to validate the synergistic impacts of symptom clusters on health-related quality of life in COPD patients compared with single symptoms. These findings, which offer a detailed understanding of symptom clusters present in COPD, provide a basis to guide efficient clinical assessment and management symptom-related distress.
A Method for Cryopreservation and Single Nucleus RNA-sequencing of Normal Adult Human Interventricular Septum Heart Tissue Reveals Cellular Diversity and Function
Authors: Larson Amy , Chin Michael T. 
Published: December 2020

Abstract
Background: Single cell sequencing of human heart tissue is technically challenging and methods to cryopreserve heart tissue for obtaining single cell information have not been standardized. Studies published to date have used varying methods to preserve and process human heart tissue, and have generated interesting datasets, but development of a biobanking standard has not yet been achieved. Heart transcription patterns are known to be regionally diverse, and there are few single cell datasets for normal human heart tissue. Methods: Using pig tissue, we developed a rigorous and reproducible method for tissue mincing and cryopreservation that allowed recovery of high quality single nuclei RNA. We subsequently tested this protocol on normal human heart tissue obtained from organ donors and were able to recover high quality nuclei for generation of single nuclei RNA-seq datasets, using a commercially available platform from 10x Genomics. We analyzed these datasets using standard software packages such as CellRanger and Seurat. Results: Human heart tissue preserved with our method consistently yielded nuclear RNA with RNA Integrity Numbers of greater than 8.5. We demonstrate the utility of this method for single nuclei RNA-sequencing of the normal human interventricular septum and delineating its cellular diversity. The human IVS showed unexpected diversity with detection of 23 distinct cell clusters that were subsequently categorized into different cell types. Cardiomyocytes and fibroblasts were the most commonly identified cell types and could be further subdivided into 5 different cardiomyocyte subtypes and 6 different fibroblast subtypes that differed by gene expression patterns. Ingenuity Pathway analysis of these gene expression patterns suggested functional diversity in these cell subtypes. Conclusions: Here we report a simple technical method for cryopreservation and subsequent nuclear isolation of human interventricular septum tissue that can be done with common laboratory equipment. We show how this method can be used to generate single nuclei transcriptomic datasets that rival those already published by larger groups in terms of cell diversity and complexity and suggest that this simple method can provide guidance for biobanking of human myocardial tissue for complex genomic analysis.
Emotional Fusion and Design Optimization Method Based on Kansei-AD: A Case Study of Laser Additive Manufacturing Equipment
Authors: Ma Mingyu , Jiang Xingyu , Liu Weijun , Wang Xu , Zhao Chen , Liu Dan , Yang Guozhe 
Published: December 2020

Abstract
To improve the level of integration between the psychological and emotional needs of laser additive manufacturing equipment personnel and the cognitive interactive design of human-machine systems, an emotional optimization design method for laser additive manufacturing equipment based on Kansei engineering and axiomatic design (Kansei-AD) is proposed. By exploring the emotional cognitive process of personnel on equipment, the representation means of the psychological and emotional needs of personnel are clarified. To clarify the psychological and emotional needs of users, the Kansei semantic difference experiment is applied to determine the user domain of axiomatic design. To resolve the difficulty of accurately mapping and expressing between user emotional factors and design elements in the design process, a combination of rough analytic hierarchy process (rough AHP) and quality function deployment (QFD) is used in the functional domain to determine the user Kansei design needs. In addition, the mapping relationship between Kansei design requirements and design requirements is judged by independent axioms of axiomatic design. A matrix of morphological elements-design requirements is constructed, and the morphological design elements that meet the psychological and emotional needs of personnel are determined using the GRA-TOPSIS method. LDM4030 laser coaxial powder feeding equipment is taken as an example to verify the feasibility of the optimization method. The result shows that the method effectively optimizes the design of the equipment according to the psychological and emotional needs of personnel. It provides a new research idea for the psychological and emotional design of laser additive manufacturing equipment.
Significance of Frailty in Prognosis After Surgery in Patients with Pancreatic Ductal Adenocarcinoma
Authors: Yamada Shinichiro , Shimada Mitsuo , Morine Yuji , Imura Satoru , Ikemoto Tetsuya , Saito Yu , Miyazaki Katsuki , Tokunaga Takuya , Nishi Masaaki 
Published: December 2020

Abstract
Background: Frailty is an important consideration for older patients undergoing surgery. We aimed to investigate whether frailty could be prognostic factor in patients with pancreatic ductal adenocarcinoma who underwent pancreatic resection.Methods: One hundred and twenty patients who underwent pancreatic resection for pancreatic ductal adenocarcinoma were enrolled. Frailty was defined as a clinical frailty scale score ≥4. Patients were divided into frailty (n = 29) and non-frailty (n=91) groups, and clinicopathological factors were compared between two groups. Results: The frailty group showed an older age, lower serum albumin concentration, lower prognostic nutritional index, larger tumor diameter, and higher rate of lymph node metastasis than the non-frailty group (p < 0.05). Neutrophil–lymphocyte ratio and modified Glasgow prognostic score tended to be higher in the frailty group. Cancer-specific and disease-free survival rates were significantly poor in the frailty group (p < 0.05). With a multivariate analysis, frailty was an independent prognostic factor of cancer-specific survival.Conclusions: Frailty can predict the prognosis of patients with pancreatic ductal adenocarcinoma who undergo pancreatic resection.
The Knowledge and Attitudes of Health Professionals Working in Mother-Friendly Hospitals About Complementary Therapy and Supportive Care Methods
Authors: Bolsoy Nursen , Tayhan Esra Bozhan , Durgun Secil Köken , Damar Elif , Kayıp Emine 
Published: December 2020

Abstract
Background: It is important that the healthcare professionals who are with the mother at the moment of birth and afterwards know and apply effective complementary treatment and supportive care methods, their effects and limitations. The aim of this study is to determine the knowledge levels and attitudes of health personnel about complementary therapy and supportive care methods to be used in the management of labor pain and postpartum period.Methods: A descriptive study included 142 midwives / nurses and physicians working in the delivery room and maternity services of mother-friendly hospitals in Manisa. The questionnaire form consisting of 25 questions evaluated the knowledge and attitudes of the participants about complementary therapy and supportive care methods.Results: It was found that 30.6% of the healthcare professionals participating in the study used complementary treatment and supportive care methods in their patients. The least heard method was chiropractic (81.0%), the best known method was hydrotherapy (water birth) (5.8%). The most used method is massage with a rate of 14.0%. It was determined that the mean total attitude score of the healthcare workers on complementary treatment and supportive care methods was 18.57 ± 5.12 (min: 8, max: 40). A statistically significant difference was found between the education level and the institution they work for of healthcare professionals and their attitude scores (p <0.05). Conclusions: 1 out of every two healthcare professionals did not have information about complementary treatment and supportive care methods. It can be said that the use of these methods will increase with the learning desire and positive attitude of healthcare professionals.
Investigating The Relationship Between&nbsp;Insomnia,&nbsp;Menopausal Syndrome and Depression and Exploring&nbsp;The Therapeutic Effects of Jieyu Pill
Authors: Wang Hui , Hou Xucan , Tian Xuemei , Wang Yun 
Published: December 2020

Abstract
Background: This paper explores the effects of insomnia and menopausal syndrome on the depressive process at the cellular and molecular levels, and investigates the mechanisms by which the commonly used antidepressant formula, Jieyu Pill (JYP), also has efficacy in insomnia and menopausal syndrome. Methods: Neo4j was used to construct a graph database based on three diseases and mechanism of JYP; PPI data were used to analyze the molecular mechanism of effects of the diseases on depression process; the entity grammar system was used to resolve the mechanism of JYP in treatment of two diseases. Results: A database of JYP and three disease charts was constructed. The relationship between insomnia and menopausal syndrome in terms of molecular mechanisms and cellular communication on the depressive process was obtained. The mechanism of JYP in insomnia and menopausal syndrome was obtained. Conclusions: The results of this paper, which was conducted from the point of view of cellular communication and molecular mechanisms, show that insomnia and menopausal syndrome can have an impact on the course of depression. Therefore, synergistic treatment should be given to patients with depression associated with these two disorders. JYP is effective for all three disorders, can comprehensively regulate the organism and has high compliance, making it a clinical formula worth promoting.
WITHDRAWN: Healthcare Professional Association Agency In Preparing For Artificial Intelligence: A Multi-Case Study Protocol
Authors: Gillan Caitlin , Hodges Brian , Wiljer David , Dobrow Mark 
Published: December 2020

Abstract
BackgroundThe emergence of artificial intelligence (AI) in healthcare impacts on most aspects of our healthcare systems including consideration of employment, training, education, and professional regulation for healthcare professions. It is incumbent on healthcare professional associations (HPAs) to assist their membership in defining and preparing for AI-related change. HPAs, or the national groups convened to represent the interests of the members of a profession, hold a unique role in establishing and maintaining the socio-cultural, normative, and regulative elements of healthcare professions. The objective of this study is to examine how, when faced with AI as a disruptive technology, HPAs engage in sense-making and legitimization of change in order to support their membership in preparing for future practice.MethodsAn exploratory multi-case study approach will be employed. This study will be informed by normalization process theory (NPT), which suggests behavioural constructs required for complex change, providing a novel lens through which to consider the agency of macro-level actors in practice change. Four HPAs will be studied, with each representing an instrumental case and related fields, selected for their early consideration and adoption of AI technologies. Data collection will consist of key-informant interviews, observation of relevant meetings, and document review. Individual and collective sense-making activities and action toward change will be identified and characterized using stakeholder network mapping. A hybrid inductive/deductive model will be used for a concurrent thematic analysis, mapping emergent themes against the NPT framework to assess fit and identify areas of discordance. DiscussionA linear cross-case analytic approach will be taken to present the data, highlighting both guidance for the implementation of AI and implications for the application of NPT at the macro level. The ability to inform consideration of AI will depend on the degree to which the engaged HPAs have considered this topic at the time of the study, and hence what priority it has been assigned within the HPA and what actions have been taken to consider or prepare for it. The fact that this may differ between HPAs and associated professions and practice environments will require consideration throughout the analysis. Ultimately, this protocol outlines a case study approach to understanding how, when faced with AI as a disruptive technology, HPAs engage in sense-making and legitimization of the change in order to support their membership in preparing for future practice.
Subtyping Salmonella of Pet Dogs With Multilocus Sequence Typing (MLST) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs)
Authors: Yang Cheng , Shao Wangfeng , Wei Lingling , Chen Lingxiao , Zhu Aihua , Pan Zhiming 
Published: December 2020

Abstract
Salmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China, and used multilocus sequence typing（MLST）and clustered regularly interspaced short palindromic repeats（CRISPRs）to subtype Salmonella isolates. From April 2018 to November 2019, a total of 469 samples were collected from pet hospitals and breeding base, including 339 dog samples and 60 cat samples. S. Kentucky (40.74%) was the most prevalent serotype, but other, such as S. Typhimurium (18.52%) and S. Indiana (18.52%), were also widespread. Eight different sequence type (ST) patterns were Identified by MLST and ST198 was the highest proportion of these isolates. CRISPRs analysis showed that 9 different Kentucky CRISPR types (KCTs) was identified from ST198. 48 spacers including 29 (6 News) for CRISPR1 and 19 (4 News) for CRISPR2 that proved the polymorphic of Salmonella genes in samples from different sources. The analysis demonstrated that the common serotypes were widely present in pet hosts in the same area. This analysis shows that CRISPR genes have better recognition ability in the same serotype, which has a positive effect on the traceability of salmonella and the prevention and treatment of salmonellosis.
Quercetin Prevents Primordial Follicle Loss via Suppression of PI3K/Akt/Foxo3a Pathway Activation in Cyclophosphamide - Treated Mouse
Authors: Li Jianghui , Long Hui , Cong Yanyan , Gao Hongyuan , Lyu Qifeng , Yu Sha , Kuang Yanping 
Published: December 2020

Abstract
Background: Chemotherapy improves survival rates but often causes some adverse effects associated with ovarian damage, characterized by a decreased of primordial follicle stockpiles. Recent studies reveal that chemotherapy may stimulate the PI3K signaling pathway, who has roles in manipulating the dormancy and activation of mammalian primordial follicles, resulting in accelerated primordial follicles activation followed by the loss of ovarian reserve. As an inhibitor of PI3K pathway, whether quercetin has protective properties against chemotherapy - induced follicle loss in mice is worth to be explored.Methods: The effects of quercetin on the mouse model of cyclophosphamide-induced ovarian dysfunction were investigated. Paraffin sections of mouse ovary were stained with hematoxylin and eosin for differential follicles count and TUNEL assay for apoptosis detection. Immunohistochemistry stain with ki67 and Foxo3a were used to evaluate the activation of primordial follicles. The function of PI3K signaling pathway were assessed via the western blot of ovary.Results: Quercetin cotreatment rescued the reduction number of dormant primordial follicles induced by cyclophosphamide. Moreover, analysis of the PI3K/Akt/Foxo3a pathway demonstrated that quercetin co - administration decreased phosphorylation of proteins that stimulate follicle activation in ovary induced by cyclophosphamide. Meanwhile, Quercetin prevents cyclophosphamide - induced apoptosis in early growing follicles and early antral follicles, maintaining AMH level secreted by these follicles, preserving the quiescence of the primordial follicle pool, characterized by the intranuclear staining of Foxo3a in primordial follicle. Conclusions: Quercetin attenuates cyclophosphamide - induced follicle loss by preventing the phosphorylation of PI3K/Akt/Foxo3a pathway members and maintaining AMH level secreted by growing follicles.
Herd immunity of Covid 19 in dynamic environments with vaccination: a model based study
Authors: Yang Liwei , Chen Bochuan , Zhang Yu , Du Siyao , Zhao Lang 
Published: December 2020

Abstract
BackgroundThe first wave of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Canada is entering the last stage, while the development of vaccine is still ongoing. A thorough analysis on the potential effect of restoring to the normal life was needed.MethodsWe used an infectious disease model which optimized for individual immunity to investigate the potential impact of the vaccine on the number of cases, ℛ𝑡, and the duration of the epidemic. We modeled the severity of the cases with three intervention measures and the effect of herd immunity. The combined intervention strategies with the vaccination, speed of vaccination, and the proportion of population pre-vaccinated before reopening were modeled to give an overview of the effect of the vaccination. For each simulation, we set the observation range to be from Feb, 2020 to Oct. 2021, and modeled the number of cases after the first wave, the change of reproduction number (ℛ𝑡), and the proportion of immunized population under the effect of waning immunity.FindingsWe found the proportion of immunized population to reach herd immunity in a dynamic environment to be between 1−1ℛ0and 1−1ℛ02; for Covid-19, the threshold proportion is 64·16%, the final proportion of infections could be up to 87·15% when basic reproduction number (ℛ0) is 2·79. The average number of cases predicted in Canada after the first wave was 285590, 90260, 163057, and 60082 with no intervention, social distancing, quarantining severe cases, and combined strategies; 122261, 89903, 49276, 39856, and 10983 cases with 0·1%, 0·2%, 0·3%, 0·4%, and 1·0% of the population vaccinated per day; 117475, 93502, 91634, 79418, and 8713 cases with 10%, 20%, 30%, 40%, and 50% of population immunized before reopening. Assuming the half-life of the effectivity of the antibody is 48 weeks for symptomatic cases, 24 weeks for asymptomatic cases.Interpretation Neither of these strategies cannot prevent the second wave solely nor together. However, the third wave can be prevented with both social distancing and quarantining severe cases in practice. The speed and timing of vaccination has a direct impact on the reduction of the final number of cases. Unexpectedly, the proportion of population immunized before reopening did not lead to a huge shift of the number of cases after the first wave when the immunized proportion is lower than the critical proportion (49·6%) when social distancing is in practice.