bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real‐World Retrospective Audit on the Use of the TechnoClone TECHNOSCREEN ADAMTS‐13 Assay in the Diagnostic Process for Thrombotic Thrombocytopenic Purpura at LabPlus, New Zealand
Authors: Wong Stephen Hong Chun , Thalari Gabriel , Ruskova Anna , Eaddy Nicola 
Published: December 2024

ABSTRACTIntroductionThe TECHNOSCREEN ADAMTS‐13 assay (ADSC) is a new lateral flow test which is simple and quick to perform, with a high negative predictive value (NPV); it may improve the diagnostic workflow for TTP. LabPlus in Auckland, New Zealand, performs all ADAMTS13 tests in the Auckland and Northland regions. The ADSC was used at LabPlus between 2022 and 2023 as part of a protocol where results of 0 IU/mL and 0.1 IU/mL were confirmed with the TECHNOZYM ADAMTS‐13 Activity chromogenic ELISA assay (ADATS). The aim was to improve cost efficiency by reducing the need for the labour‐intensive ADATS, particularly in low probability requests. This retrospective audit analyses the efficacy and efficiency of the testing protocol.MethodsAll ADAMTS‐13 tests performed in the year before the ADSC (March 2021–March 2022) and after the ADSC protocol was introduced (March 2022–March 2023) were collected from the LabPlus laboratory information system (LIS), and correlated with clinical information from the electronic health record. Statistical analysis was performed.ResultsNinety‐four test requests were audited. ADSC had an NPV of 100% on diagnostic samples. The protocol increased the median turn‐around time (TAT) by 18 h (p = 0.01). If an ADSC of 0.1 IU/mL was taken as a negative, the median TAT was decreased by 17 h (p = 0.0003) in diagnostic samples from LabPlus adjacent hospitals.ConclusionADSC has a high NPV but did not improve TAT when used as part of a testing protocol for our central laboratory receiving samples from a large area.
Fast and cost‐efficient species identification of Atlantic salmon (Salmo salar L.), brown trout (Salmo trutta), and their hybrids using a single SNP marker
Authors: Aykanat Tutku , Balatsou Athina , Kähkönen Kirsi , Syrjänen Jukka T. , Janhunen Matti , Leinonen Tuomas , Prokkola Jenni M. , Norrgård Johnny R. , Piccolo John J. 
Published: December 2024

AbstractA workflow for developing a cost‐ and time‐efficient, single nucleotide polymorphism (SNP)‐based assay for species and hybrid identification is described. In a reference set (n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon (Salmo salar L., n = 23) and brown trout (Salmo trutta, n = 23), with 100% accuracy. Furthermore, species and hybrid identification using field‐collected embryos had 98.1% concordance (155/158) to more expensive and time‐consuming methods that utilized multiple SNP markers. The method can be integrated into management and conservation plans to quantify species' spawning distribution and hybridization rates.
Molecular Detection of a Novel Poxvirus in Black Bullhead (Ameiurus melas): Emerging Pathogens in a Natural Freshwater in Hungary?
Authors: Abonyi Flóra , Doszpoly Andor , Czeglédi István , Eszterbauer Edit 
Published: December 2024

ABSTRACTUnexpected mass mortality of black bullhead Ameiurus melas (Rafinesque, 1820) fingerlings occurred 1 week after their arrival at our animal facility. The specimens of the invasive fish species originated from Lake Balaton, the largest natural freshwater lake in Hungary. The mortality reached 100% within 3 days. Gross pathological examination detected inflammation in the inner organs, renomegaly, and a marble, pale pink liver. Two pathogenic bacteria strains, Vibrio cholerae Pacini, 1854, and Plesiomonas shigelloides (Bader 1954), were detected in the fish liver examined. The molecular virological examination showed the presence of the nucleic acid of two dsDNA viruses. One is the Ictalurid herpesvirus 2 (IcHV‐2), which is a well‐known pathogen of the black bullhead that has only been reported from Italian fish farms previously. This is the first report of IcHV‐2 from a natural freshwater habitat. The other viral DNA detected in fish belongs to an unidentified virus species. Sequence analysis of the partial DNA polymerase gene confirmed that the novel virus is a member of the subfamily Chordopoxvirinae within the family Poxviridae. Although the causative agent of this mass mortality could not be identified with certainty, the clinical signs suggest that the detected viruses are the most suspicious. Furthermore, our results highlight that the health monitoring of invasive species is also important for the timely detection of pathogens that threaten the native fish fauna.
Microscopic and Macroscopic Analysis of Purple Sweet Potato Dried Products Following Vacuum Steam Pulsation Blanching Pretreatment
Authors: Wang Dong , Zhao Yifan , Niu Yao , Sun Haoyuan , Yuan Yuejin 
Published: December 2024

ABSTRACTTo improve the drying efficiency and quality of purple sweet potatoes after blanching pretreatment, this study employed three methods: hot water blanching (HWB), microwave blanching (MB), and vacuum steam pulsed blanching (VSPB). The impact of different blanching methods on the microstructural parameters and macroscopic performance of purple sweet potatoes was investigated using techniques such as paraffin sectioning, microscopic observation, and image processing. The results indicated that, at the microscopic level, the proportion of large cells after VSPB was significantly higher than that after hot water and MB, being 15.81%, 5.15%, and 25.68%, respectively. At the macroscopic level, samples pretreated with VSPB had the shortest drying time (210 min), the highest rehydration ratio (RR) (3.988), and the best chewiness (2095.44 g). Further studies explored the effects of blanching vacuum degree, blanching time, and blanching frequency on the distribution of various microstructural parameters of purple sweet potato tissue cells. Through nonlinear polynomial fitting, a fitting equation was established between the microstructural parameters after VSPB and the macroscopic RR and browning index. The determination coeffi cients R2 of the established mathematical models and the predicted results were all greater than 0.8. The findings of this study provide an important reference for exploring the mechanism of the impact of VSPB pretreatment on the microstructural parameters and macroscopic performance of purple sweet potatoes and offer a theoretical basis for the design of drying equipment.
Relationship Between Fear of Childbirth and Oral Health‐Related Quality of Life in Pregnant Women: The Mediating Effect of Pregnancy Anxiety
Authors: Chen Yujia , Zhao Jing , Zheng Jin , Zou Zhijie , Chen Xiaoli 
Published: December 2024

ABSTRACTBackgroundPsychosocial health affects oral health‐related quality of life. Pregnancy, as a special time for women, produces significant psychological changes. However, the relationship between mental health during pregnancy and oral health‐related quality of life is unclear.ObjectiveThe study aims to explore the association between fear of childbirth and investigate the mediating effect of pregnancy anxiety.MethodsUsing a cross‐sectional design, 478 pregnant women were recruited and evaluated using the Fear of Birth Scale, the Pregnancy‐Related Anxiety Questionnaire and the Oral Health Impact Profile‐14. Multiple linear regressions were performed to evaluate the relationship between fear of childbirth and oral health‐related quality of life. PROCESS software was applied to assess the mediating effect of pregnancy anxiety.ResultsBoth fear of childbirth and pregnancy anxiety were negative correlation with oral health‐related quality. Pregnancy anxiety mediated 40% of the association between fear of childbirth and oral health‐related quality of life (β = 0.072, SE = 0.030, 95% CI [0.015, 0.131]).ConclusionsPregnancy anxiety mediates the relationship between fear of childbirth and oral health‐related quality of life in pregnant women. To improve the oral health‐related quality of life among pregnant women, relevant healthcare workers should actively help pregnant women to improve psychological health.
Alpine refugial isolation drove divergence of the cold‐adapted montane scorpionfly Cerapanorpa obtusa (Mecoptera: Panorpidae)
Authors: Gao Kai , Hua Yuan , He Le‐Le , Hua Bao‐Zhen 
Published: December 2024

AbstractQuaternary climate fluctuations and complex mountain systems had a prominent impact on the genetic diversification and speciation of montane organisms. However, the genetic imprints of the interplay between past climate events and rugged relief on montane species remain largely unresolved. Here, we analyzed the phylogeny, population structure, divergence time, demographic simulations, and ecological niche modeling of the montane scorpionfly Cerapanorpa obtusa (Cheng) from 36 populations in the mountains of central China (MCC) using three mitochondrial and two nuclear genes to explore its evolutionary history. The results show that C. obtusa originated from the Minshan Mountains, and currently consists of six genetically fragmented lineages that diverged 1.25–0.52 Ma. Four minor lineages (S1−S4) are confined to the topographically rugged Minshan Mountains, and two major lineages (NW and NE) are widely distributed in the northern MCC with relatively homogeneous landscapes. The Minshan Mountains are likely interglacial microrefugia for C. obtusa, and as spatial buffers in response to past climate changes. These findings provide some evidence that the interplay between climate changes and rugged relief may play a significant role in shaping the distinct phylogeographical pattern of cold‐adapted montane insects. These results would also seem to suggest the importance of topographically rugged mountain systems in the conservation of evolutionary diversity and endemic species.
Governance Index for Prioritisation of Afrotropical Inland Lakes for Conservation and Sustainability
Authors: Aura Christopher Mulanda , Musa Safina , Owiti Horace Onyango , Awour Fonda Jane 
Published: December 2024

ABSTRACTExisting scientific methods can be combined with insights on the socio‐ecological landscape of Afrotropical lakes to provide better policy options for stakeholders and to develop more efficient resource management strategies. Using integrated African resource and ecosystem maps and an approach for the developed Governance Index for Prioritisation (GIP) of Lake Victoria resources, our study highlights prospects for adopting the GIP methodology to increase understanding and management of Afrotropical lakes. The use of GIP is recommended for adoption and promotion in areas where such approaches appear defensible for the assessment of lake‐wide practices, and in areas with robust indigenous knowledge to provide a broad view of the ecological health of the Afrotropical ecosystem.
A Novel Tool for Soybean Pods and Branches Recognition by Deep Learning Based Approach
Authors: Xie Qize , Du Hongbo , Yang Qing , Liu Zhi , Jin Xin , Li Chuanfeng , Wei Yu , Tang Futong , Tao Peijun , Yan Long 
Published: December 2024

ABSTRACTPhenotypic trait identification is crucial in cultivating new soybean varieties with high yield and quality. The traditional soybean phenotypic trait identification relies on manual pod counting and plant height measuring with a ruler. The heavy workload causes the data collected by human resources to be extremely prone to error. Therefore, developing an efficient and high‐quality method to obtain phenotypic data of soybean pods and branches is urgently needed. Three network models including ResNet‐101, Swin‐S and ConvNeXt‐S are compared in this study, and the ConvNeXt‐S model is identified as optimal, with a mAP@0.5 of 0.95, which could reach 74.2%. A deep learning–based approach soybean plant phenotype detection and data storage system is developed, including information on plants, pods and branches. The R2 between the number of pods detected by the system and the true value reached 0.995. These results indicate that the system is more accurate and stable than the manual phenotype identification. Our study paves the way for reducing economic and time costs as well as improving phenotype identification efficiency and accuracy in detecting soybean phenotypes.
Optimization of Aerobic Synthesis of Fumaric Acid from Glucose by a Recombinant Escherichia coli Strain Functioning in a Whole-Cell Biocatalyst Mode
Authors: Skorokhodova A. Yu. , Stasenko A. A. , Gulevich A. Yu. 
Published: December 2024

Abstract
Biocatalytic synthesis of fumaric acid from glucose by the previously engineered E. coli strain FUM1.0 (pMW119-kgd) (E. coli MG1655 ∆ackA-pta, ∆ldhA, ∆adhE, ∆ptsG, PL-glk, Ptac-galP, ∆fumB, ∆fumAC, poxB::PL-pycABs, pMW119-kgd) was optimized. The maximal yield of the target substance was achieved upon its synthesis through a variant of the tricarboxylic acid cycle mediated by the action of heterologous 2-ketoglutarate decarboxylase. The enhanced expression of the genes encoding components of the succinate dehydrogenase enzymatic complex did not markedly affect the biosynthetic characteristics of the producing strain. A positive effect of decreasing the intracellular ATP supply on the conversion of the carbohydrate substrate into the target product was demonstrated. The activation of the futile cycle of pyruvate–phosphoenolpyruvate–pyruvate due to an increase in the expression of the ppsA gene led to a slight increase in the yield of fumaric acid. Upon uncoupling the H+-ATP synthase complex subunits resulting in ATP formation cessation via oxidative phosphorylation, due to deletion of the atpFH genes, the molar yield of fumaric acid from glucose demonstrated by the strain functioning in the whole-cell biocatalyst mode reached 92%.
Comparison of Genomes, Resistomes, and Antimicrobial Properties of the Three Lactococcus lactis Strains from Fermented Milk and Cereal Products of South Africa and Russia
Authors: Moiseenko K. V. , Glazunova O. A. , Savinova O. S. , Fedorova T. V. 
Published: December 2024

Abstract
Whole-genome sequencing was carried out, and the main characteristics of the genomes of three new strains of L. lactis AM1, MA1, and dlA, isolated from fermented milk and cereal grain products of South Africa and Russia, were established. The dual approach of in vitro and in silico analysis used in this work allowed us to assess more fully the bacteriocinogenic potential and antibiotic resistance of L. lactis strains. It was shown that L. lactis AM1 and dlA had an antagonistic effect against the test cultures of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 6538. The growth retardation of E. coli and S. aureus after 48 hours of cocultivation was 65–75 and 30–50%, respectively. The L. lactis MA1 strain did not exhibit a significant antagonistic effect; the growth inhibition of E. coli and S. aureus was 13 and 26%, respectively. According to the results of in silico analysis using the BAGELv4.0 service, gene clusters of class IId lactococcin-like bacteriocins were found in the genomes of all three strains. In the genomes of the dlA and AM1 strains, the sactipeptide cluster, a class I bacteriocin, is additionally annotated. In silico analysis of the L. lactis genomes showed the absence of transmissible antibiotic resistance genes, and all three strains showed a phenotype susceptible to the main classes of clinically relevant antibiotics (ampicillin, streptomycin, kanamycin, gentamicin, erythromycin, clindamycin, vancomycin, tetracycline, and chloramphenicol). Taken together, the results provide valuable information about the intraspecific diversity of L. lactis and indicate the promise of using new strains as starter cultures and biopreservatives.
Afamin Ameliorates Testosterone Propionate (TP)-Induced Oxidative Stress and Mitochondrial Damage in Human Ovarian Granulosa Tumor Cells (KGN) by Upregulating the Expression of SIRT1
Authors: Ma Y. L. , Li Z. W. , Wang Z. , Yang A. J. 
Published: December 2024

Abstract
Polycystic ovary syndrome (PCOS) is a common endocrine disorder affecting women of reproductive age. Considerable research has confirmed that afamin is associated with the prevalence and development of metabolic syndrome. Thus, this study investigated the mechanism of action of afamin in PCOS and its potential therapeutic value. We found that PCOS patients had higher levels of afamin than normal control subjects. Afamin significantly enhanced the overall antioxidant ability and activity of antioxidant enzymes and reduced the levels of 8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA), reactive oxygen species (ROS) and superoxide anion ($${\text{O}}_{2}^{{\centerdot {\kern 1pt} - }}$$) in human ovarian granulosa tumor cells (KGN cells). In addition, afamin was also found to protect KGN cells against testosterone propionate (TP)-induced mitochondrial damage and apoptosis. Additionally, silencing of SIRT1 revealed that SIRT1 protected KGN cells against TP-induced oxidative stress (OS) injury, mitochondrial damage and apoptosis. Furthermore, this study showed that besides restoring the estrous cycle in PCOS mice, afamin might also reduce the increased cycling testosterone (T) and luteinizing hormone (LH) levels and LH/Follicle-stimulating hormone (FSH) ratio, as well as decrease the number of cystic follicles, indicating the significance of afamin in the treatment of PCOS. Moreover, afamin reduced oxidative damage in PCOS mice by enhancing their antioxidant capacity. Also, afamin may protect KGN cells against TP-induced OS by enhancing their antioxidant ability, restoring mitochondrial function, and inhibiting cell apoptosis by upregulating the expression of SIRT1. Thus, afamin may play a protective role in PCOS mice.
Revisiting medical oaths: how student-driven ethical codes reflect changing values
Authors: Stein Michael , Gruenbaum Shaun Evan , Jotkowitz Alan 
Published: December 2024

BackgroundMedical oaths and ethical codes play a crucial role in guiding physicians through their professional responsibilities. This study extends prior research on ethical codes created by students at the Medical School for International Health (MSIH) by analysing codes from the years 2007 to 2022.ObjectiveThe objective of this study is to evaluate changes in the ethical principles emphasised by MSIH students over time and to compare these with previous cohorts.MethodsEthical codes from MSIH classes (2007–2022) were analysed using the framework developed by Baker. This framework categorises oaths into four sections: preamble, covenant, code of duties to patients and peroration. The focus was primarily on the code of duties to patients. Quantitative analysis was used to assess the prevalence of specific ethical principles.ResultsThe study found an increased emphasis on principles such as ‘respect for patients’ and ‘cultural competence/sensitivity’, while traditional values like ‘benevolence’ and ‘honesty’ were mentioned less frequently. New themes, including ‘patient autonomy’ and ‘cultural competence’, emerged, reflecting a shift towards patient-centred care and justice, though emerging issues like artificial intelligence ethics were not addressed.ConclusionsMSIH students’ ethical codes have evolved to emphasise patient respect and cultural competence. The findings suggest a maturation in the ethical perspectives of medical students, though gaps remain in addressing contemporary challenges. Future studies should explore how ethical codes can incorporate emerging issues in healthcare.
Perfil emprendedor en estudiantes de educación superior chilenos
Authors: Quintano-Méndez Felipe , Riquelme-Segura Leonor , Morales-Pizarro Jorge , Balmaceda-Gallardo Ingrid , Manríquez-Bravo Gerald 
Published: December 2024

(analytical)Entrepreneurship currently has a relevant position in vocational training. This study sought to identify entrepreneurial profiles among students in the Faculty of Business and Administration at the Instituto Profesional de Chile. A non-probabilistic sample of 240 students was used. The results of the research showed a high level of entrepreneurial behaviors, subjective norms, self-belief and entrepreneurial intentions, as well as the identification of four different profiles: natural entrepreneur, informal entrepreneur, entrepreneur due to necessity and non-entrepreneur. These results provide new information about entrepreneurship among young people that can be used to inform educational processes, pedagogical practices, teacher training, developing links with local settings and the design of graduate profiles for business and administration degrees.
Keywords: Entrepreneurship; competence development; vocational education; youth; quantitative analysis.
Europeanization in 21st Century Hong Kong Written Chinese (HKWC)
Authors: Liang Minhua 
Published: December 2024

Abstract
Europeanization of written Chinese refers to the influence of European languages on the Chinese lexicon and syntactic structures. As a regional variety, Hong Kong Written Chinese (HKWC) also possesses various Europeanized features. Most studies have spotted Europeanized features in written Chinese, whereas the consolidation of data sources and updated corpus-based analysis is required. This study fills the gap by tallying the usage frequency and examining the change in the degree of societal acceptance of the significant Europeanized features in HKWC in the 2020s. The usage frequency reveals that some of the debatable features have fallen into disuse, whereas the rest have been legitimized and incorporated as part of the normative standards in today’s HKWC. The corpus-based analysis shows that certain Europeanized features have evolved into new usage. In addition, this study also identifies semantic shift and other contact-induced phenomena resulting from Europeanization.
Freedom of Association in France: A Freedom in Danger?
Authors: Barbé Vanessa 
Published: December 2024

Abstract
Freedom of association has been an important fundamental right in France since a landmark ruling by the Constitutional Court in 1971. In that decision, the Court not only ruled that freedom of association was a constitutional right that was not explicitly mentioned in the Constitution, but also that the Constitutional Court could overrule an Act of Parliament if it was incompatible with such a fundamental constitutional right. That kind of ‘Marbury v Madison’ decision was a small revolution in France. In 1971, it was the first time that a piece of legislation was annulled by the Constitutional Court because it was incompatible with a fundamental right when Parliament wanted to change the system of declaration of associations into a system of authorisation. Nevertheless, since 2017, there has never been such a high number of dissolutions of associations. Moreover, since the statute against separatism in August 2021, there has been a new ground to dissolve associations that “provoke armed demonstrations or acts of violence against persons or property.” The change in the law in 2021 has led to a marked increase in the number of dissolutions, which might lead one to believe that freedom of association is under threat in France.
Notes on the Force of Black Domesticity
Authors: Jordan Taryn D. 
Published: December 2024

Abstract
Drawing on histories of black domesticity, Simone de Beauvoir’s writings about woman’s situation in housework, and Angela Y. Davis’s refutation of the myth of the black matriarch, this article argues that the force of the black domestic is elusive because it cannot be understood through immanence and transcendence. Through an alternative logic based on black female presence that exceeds the confines of the human, the force of black domesticity emerges as a proliferation of objects that endure the test of time.