bluuebunny/crossref_metadata_2025_split huggingface dataset:
UK Internet antenatal dietary advice: a content accuracy and readability analysis
Authors: Sidnell Anne , Nestel Penelope 
Published: June 2020

AbstractThe aim of the study was to assess the accuracy and readability of Internet prenatal nutrition advice. Between August and December 2018, 130 Internet pages returned from Google searches on foods to avoid, foods to eat and supplements use were compared with UK government advice for pregnant women. Readability was assessed using the Flesch Readability Ease (FRE) tool. Descriptive and non-parametric tests were used. Spearman’s correlation explored associations between accuracy and readability. Kruskal–Wallis tests with Bonferroni correction were used for multiple pairwise tests and Mann–Whitney U tests for two-sample differences in medians. A total of 130 Internet pages were examined: 48 % from publishers, 27 % from other commercial organisations, 22 % from charities and 3 % from governments. Eighty-three (64 %) pages contained inaccurate and accurate advice, twenty-three (18 %) were accurate and complete, twenty-one (16 %) were inaccurate, and three (2 %) lacked any relevant advice. The median percentage accuracy of all advice was 83 (lower quartile, upper quartile: 48, 100). Median FRE was 55 (46, 61) ‘fairly difficult’. Eighty-seven pages (67 %) scored below the recommended FRE for public Internet pages. There was a weak positive correlation between accuracy and readability of Internet pages (rho = 0·241, P = 0·006). Accuracy of Internet pages did not differ by dietary theme. Pages on supplements were the most difficult to read. Internet pages from publishers and other commercial organisations were significantly less accurate than those from not-for-profit organisations (median percentage difference –8 (–29, 0·00), P = 0·019). Much pregnancy-related dietary advice online is inaccurate and difficult to read. Advice should be developed in consultation with qualified nutritionists and dietitians.
Using environmental niche modelling to investigate abiotic predictors of crocodilian attacks on people
Authors: Powell George , Versluys Thomas M. M. , Williams Jessica J. , Tiedt Sonia , Pooley Simon 
Published: June 2020

AbstractCrocodilians are distributed widely through the tropics and subtropics, and several species pose a substantial threat to human life. This has important implications for human safety and crocodilian conservation. Understanding the drivers of crocodilian attacks on people could help minimize future attacks and inform conflict management. Crocodilian attacks follow a seasonal pattern in many regions, but there has been limited analysis of the relationship between attack occurrence and fine-scale contemporaneous environmental conditions. We use methods from environmental niche modelling to explore the relationships between attacks on people and abiotic predictors at a daily temporal resolution for the Nile crocodile Crocodylus niloticus in South Africa and Eswatini (formerly Swaziland), and the American alligator Alligator mississippiensis in Florida, USA. Our results indicate that ambient daily temperature is the most important abiotic temporal predictor of attack occurrence for both species, with attack likelihood increasing markedly when mean daily temperatures exceed 18 °C and peaking at 28 °C. It is likely that this relationship is explained partially by human propensity to spend time in and around water in warmer weather but also by the effect of temperature on crocodilian hunting behaviour and physiology, especially the ability to digest food. We discuss the potential of our findings to contribute to the management of crocodilians, with benefits for both human safety and conservation, and the application of environmental niche modelling for understanding human–wildlife conflicts involving both ectotherms and endotherms.
Challenging the negative learning bias hypothesis of depression: reversal learning in a naturalistic psychiatric sample
Authors: Brolsma Sophie C. A. , Vrijsen Janna N. , Vassena Eliana , Rostami Kandroodi Mojtaba , Bergman M. Annemiek , van Eijndhoven Philip F. , Collard Rose M. , den Ouden Hanneke E. M. , Schene Aart H. , Cools Roshan 
Published: June 2020

AbstractBackgroundClassic theories posit that depression is driven by a negative learning bias. Most studies supporting this proposition used small and selected samples, excluding patients with comorbidities. However, comorbidity between psychiatric disorders occurs in up to 70% of the population. Therefore, the generalizability of the negative bias hypothesis to a naturalistic psychiatric sample as well as the specificity of the bias to depression, remain unclear. In the present study, we tested the negative learning bias hypothesis in a large naturalistic sample of psychiatric patients, including depression, anxiety, addiction, attention-deficit/hyperactivity disorder, and/or autism. First, we assessed whether the negative bias hypothesis of depression generalized to a heterogeneous (and hence more naturalistic) depression sample compared with controls. Second, we assessed whether negative bias extends to other psychiatric disorders. Third, we adopted a dimensional approach, by using symptom severity as a way to assess associations across the sample.MethodsWe administered a probabilistic reversal learning task to 217 patients and 81 healthy controls. According to the negative bias hypothesis, participants with depression should exhibit enhanced learning and flexibility based on punishment v. reward. We combined analyses of traditional measures with more sensitive computational modeling.ResultsIn contrast to previous findings, this sample of depressed patients with psychiatric comorbidities did not show a negative learning bias.ConclusionsThese results speak against the generalizability of the negative learning bias hypothesis to depressed patients with comorbidities. This study highlights the importance of investigating unselected samples of psychiatric patients, which represent the vast majority of the psychiatric population.
‘Turning’ everywhere in IR: on the sociological underpinnings of the field's proliferating turns
Authors: Baele Stephane J. , Bettiza Gregorio 
Published: June 2020

AbstractIn the past two decades, calls for International Relations (IR) to ‘turn’ have multiplied. Having reflected on Philosophy's own linguistic turn in the 1980s and 1990s, IR appears today in the midst of taking – almost simultaneously – a range of different turns, from the aesthetic to the affective, from the historical to the practice, from the new material to the queer. This paper seeks to make sense of this puzzling development. Building on Bourdieu's sociology of science, we argue that although the turns ostensibly bring about (or resuscitate) ambitious philosophical, ontological, and epistemological questions to challenge what is deemed to constitute the ‘mainstream’ of IR, their impact is more likely to be felt at the ‘margins’ of the discipline. From this perspective, claiming a turn constitutes a position-enhancing move for scholars seeking to accumulate social capital, understood as scientific authority, and become ‘established heretics’ within the intellectual subfield of critical IR. We therefore expect the proliferation of turns to reshape more substantively what it means to do critical IR, rather than turning the whole discipline on its head.
Depth‐Dependent Azimuthal Anisotropy Beneath the Juan de Fuca Plate System
Authors: Eilon Zachary C. , Forsyth Donald W. 
Published: June 2020

AbstractWe use surface wave measurements to reveal anisotropy as a function of depth within the Juan de Fuca and Gorda plate system. Using a two‐plane wave method, we measure phase velocity and azimuthal anisotropy of fundamental mode Rayleigh waves, solving for anisotropic shear velocity. These surface wave measurements are jointly inverted with constraints from SKS splitting studies using a Markov chain approach. We show that the two data sets are consistent and present inversions that offer new constraints on the vertical distribution of strain beneath the plates and the processes at spreading centers. Anisotropy of the Juan de Fuca plate interior is strongest (~2.4%) in the low‐velocity zone between ~40‐ to 90‐km depth, with ENE direction driven by relative shear between plate motion and mantle return flow from the Cascadia subduction zone. In disagreement with Pn measurements, weak (~1.1%) lithospheric anisotropy in Juan de Fuca is highly oblique to the expected ridge‐perpendicular direction, perhaps connoting complex intralithospheric fabrics associated with melt or off‐axis downwelling. In the Gorda microplate, strong shallow anisotropy (~1.9%) is consistent with Pn inversions and aligned with spreading and may be enhanced by edge‐driven internal strain. Weak anisotropy with ambiguous orientation in the low‐velocity zone can be explained by Gorda's youth and modest motion relative to the Pacific. Deeper (≥90 km) fabric appears controlled by regional flow fields modulated by the Farallon slab edge: anisotropy is strong (~1.8%) beneath Gorda, but absent beneath the Juan de Fuca, which is in the strain shadow of the slab.
Variational Phase‐Field Modeling of Hydraulic Fracture Interaction With Natural Fractures and Application to Enhanced Geothermal Systems
Authors: Lepillier Baptiste , Yoshioka Keita , Parisio Francesco , Bakker Richard , Bruhn David 
Published: June 2020

AbstractIn every tight formation reservoir, natural fractures play an important role for mass and energy transport and stress distribution. Enhanced Geothermal Systems (EGS) make no exception, and stimulation aims at increasing the reservoir permeability to enhance fluid circulation and heat transport. EGS development relies upon the complex task of predicting accurate hydraulic fracture propagation pathway by taking into account reservoir heterogeneities and natural or preexisting fractures. In this contribution, we employ the variational phase‐field method, which handles hydraulic fracture initiation, propagation, and interaction with natural fractures and is tested under varying conditions of rock mechanical properties and natural fractures distributions. We run bidimensional finite element simulations employing the open‐source software OpenGeoSys and apply the model to simulate realistic stimulation scenarios, each one built from field data and considering complex natural fracture geometries in the order of a thousand of fractures. Key mechanical properties are derived from laboratory measurements on samples obtained in the field. Simulations results confirm the fundamental role played by natural fractures in stimulation's predictions, which is essential for developing successful EGS projects.
Optoelectronic parametric oscillator
Authors: Hao Tengfei , Cen Qizhuang , Guan Shanhong , Li Wei , Dai Yitang , Zhu Ninghua , Li Ming 
Published: June 2020

AbstractOscillators are one of the key elements in various applications as a signal source to generate periodic oscillations. Among them, an optical parametric oscillator (OPO) is a driven harmonic oscillator based on parametric frequency conversion in an optical cavity, which has been widely investigated as a coherent light source with an extremely wide wavelength tuning range. However, steady oscillation in an OPO is confined by the cavity delay, which leads to difficulty in frequency tuning, and the frequency tuning is discrete with the minimum tuning step determined by the cavity delay. Here, we propose and demonstrate a counterpart of an OPO in the optoelectronic domain, i.e., an optoelectronic parametric oscillator (OEPO) based on parametric frequency conversion in an optoelectronic cavity to generate microwave signals. Owing to the unique energy-transition process in the optoelectronic cavity, the phase evolution in the OEPO is not linear, leading to steady single-mode oscillation or multimode oscillation that is not bounded by the cavity delay. Furthermore, the multimode oscillation in the OEPO is stable and easy to realize owing to the phase control of the parametric frequency-conversion process in the optoelectronic cavity, while stable multimode oscillation is difficult to achieve in conventional oscillators such as an optoelectronic oscillator (OEO) or an OPO due to the mode-hopping and mode-competition effect. The proposed OEPO has great potential in applications such as microwave signal generation, oscillator-based computation, and radio-frequency phase-stable transfer.
USP42 enhances homologous recombination repair by promoting R-loop resolution with a DNA–RNA helicase DHX9
Authors: Matsui Misaki , Sakasai Ryo , Abe Masako , Kimura Yusuke , Kajita Shoki , Torii Wakana , Katsuki Yoko , Ishiai Masamichi , Iwabuchi Kuniyoshi , Takata Minoru , Nishi Ryotaro 
Published: June 2020

AbstractThe nucleus of mammalian cells is compartmentalized by nuclear bodies such as nuclear speckles, however, involvement of nuclear bodies, especially nuclear speckles, in DNA repair has not been actively investigated. Here, our focused screen for nuclear speckle factors involved in homologous recombination (HR), which is a faithful DNA double-strand break (DSB) repair mechanism, identified transcription-related nuclear speckle factors as potential HR regulators. Among the top hits, we provide evidence showing that USP42, which is a hitherto unidentified nuclear speckles protein, promotes HR by facilitating BRCA1 recruitment to DSB sites and DNA-end resection. We further showed that USP42 localization to nuclear speckles is required for efficient HR. Furthermore, we established that USP42 interacts with DHX9, which possesses DNA–RNA helicase activity, and is required for efficient resolution of DSB-induced R-loop. In conclusion, our data propose a model in which USP42 facilitates BRCA1 loading to DSB sites, resolution of DSB-induced R-loop and preferential DSB repair by HR, indicating the importance of nuclear speckle-mediated regulation of DSB repair.
Snail promotes the generation of vascular endothelium by breast cancer cells
Authors: Chang Zhenyu , Zhang Yanan , Liu Jie , Zheng Yiqiong , Li Huayue , Kong Yanjun , Li Pengyun , Peng Haiwen , Shi Yajiao , Cao Bo , Ran Fang , Chen Yingjie , Song Yuhua , Ye Qinong , Ding Lihua 
Published: June 2020

AbstractA further understanding of tumor angiogenesis is urgently needed due to the limited therapeutic efficacy of anti-angiogenesis agents. However, the origin of endothelial cells (EC) in tumors remains widely elusive and controversial. Snail has been thoroughly elucidated as a master regulator of the epithelial–mesenchymal transition (EMT), but its role in endothelium generation is not yet established. In this study, we reported a new and unexpected function of Snail in endothelium generation by breast cancer cells. We showed that high Snail-expressing breast cancer cells isolated from patients showed more endothelium generated from these cells. Expression of Snail was positively correlated with endothelial markers in breast cancer patients. The ectopic expression of Snail induced endothelial marker expression, tube formation and DiI-AcLDL uptake of breast cancer cells in vitro, and enhanced tumor growth and microvessel density in vivo. Snail-mediated endothelium generation depended on VEGF and Sox2. Mechanistically, Snail promoted the expression of VEGF and Sox2 through recruiting the p300 activator complex to these promoters. We showed the dual function of Snail in tumor initiation and angiogenesis in vivo and in vitro through activation of Sox2 and VEGF, suggesting Snail may be an ideal target for cancer therapy.
Intestinal vitamin D receptor knockout protects from oxazolone-induced colitis
Authors: Shi Yongyan , Liu Ziyun , Cui Xuewei , Zhao Qun , Liu Tianjing 
Published: June 2020

AbstractCrohn’s disease (CD) and ulcerative colitis (UC) actually had different pathological mechanisms, as the former was mainly induced by Th1 and Th17 response and the latter by Th2 response. Our previous study found that oxazolone-induced Th2-mediated colitis could not be attenuated by vitamin D supplementation. This study investigated the influence of intestinal vitamin D receptor (VDR) knockout on oxazolone-induced colitis and explored the possible immunological mechanism. Intestinal VDR knockout mice had milder oxazolone-induced colitis than wildtype controls, as demonstrated by less body weight decrease and faster recovery, more intact local structure, reduced cell apoptosis, and better preserved barrier function. Th2-mediated inflammation was significantly inhibited by VDR deficiency. Meanwhile, the percentage of invariant natural killer T (iNKT) cells did not increase as much in intestinal VDR knockout mice as in wild-type controls, nor did the iNKT cells develop normally as in the controls. Intestinal VDR knockout protected against oxazolone-induced colitis in mice by blocking Th2 cell response and reducing the function of intestinal iNKT cells. Vitamin D status had no influence on the severity of colitis. This study may explain the diverse outcomes after vitamin D supplementation in literature and add some clue to the targeted therapy of IBD.
Which long noncoding RNAs and circular RNAs contribute to inflammatory bowel disease?
Authors: Lin Lihui , Zhou Gaoshi , Chen Peng , Wang Ying , Han Jing , Chen Minhu , He Yao , Zhang Shenghong 
Published: June 2020

AbstractInflammatory bowel disease (IBD), a chronic relapsing gastrointestinal inflammatory disease, mainly comprises ulcerative colitis (UC) and Crohn’s disease (CD). Although the mechanisms and pathways of IBD have been widely examined in recent decades, its exact pathogenesis remains unclear. Studies have focused on the discovery of new therapeutic targets and application of precision medicine. Recently, a strong connection between IBD and noncoding RNAs (ncRNAs) has been reported. ncRNAs include microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs). The contributions of lncRNAs and circRNAs in IBD are less well-studied compared with those of miRNAs. However, lncRNAs and circRNAs are likely to drive personalized therapy for IBD. They will enable accurate diagnosis, prognosis, and prediction of therapeutic responses and promote IBD therapy. Herein, we briefly describe the molecular functions of lncRNAs and circRNAs and provide an overview of the current knowledge of the altered expression profiles of lncRNAs and circRNAs in patients with IBD. Further, we discuss how these RNAs are involved in the nosogenesis of IBD and are emerging as biomarkers.
Mitochondrial dysfunction generates aggregates that resist lysosomal degradation in human breast cancer cells
Authors: Biel Thomas G. , Aryal Baikuntha , Gerber Michael H. , Trevino Josè G. , Mizuno Naoko , Rao V. Ashutosh 
Published: June 2020

AbstractDisrupting functional protein homeostasis is an established therapeutic strategy for certain tumors. Ongoing studies are evaluating autophagy inhibition for overcoming chemotherapeutic resistance to such therapies by neutralizing lysosomal pH. New and sensitive methods to monitor autophagy in patients are needed to improve trial design and interpretation. We report that mitochondrial-damaged breast cancer cells and rat breast tumors accumulate p53-positive protein aggregates that resist lysosomal degradation. These aggregates were localized to enzymatically-active autolysosomes that were degrading autophagosomes and the autophagic receptor proteins TAX1BP1 and NDP52. NDP52 was identified to associate with aggregated proteins and knocking down NDP52 led to the accumulation of protein aggregates. TAX1BP1 was identified to partly localize with aggregates, and knocking down TAX1BP1 enhanced aggregate formation, suppressed autophagy, impaired NDP52 autophagic degradation and induced cell death. We propose that quantifying aggregates and autophagic receptors are two potential methods to evaluate autophagy and lysosomal degradation, as confirmed using primary human tumor samples. Collectively, this report establishes protein aggregates and autophagy receptors, TAX1BP1 and NDP52, as potential endpoints for monitoring autophagy during drug development and clinical studies.
microRNA-181c-5p promotes the formation of insulin-producing cells from human induced pluripotent stem cells by targeting smad7 and TGIF2
Authors: Li Ning , Jiang Doukou , He Qian , He Fei , Li Yang , Deng Chunyan , Li Furong 
Published: June 2020

AbstractGenerating insulin-producing cells (IPCs) from human pluripotent stem cells is a promising method for studying the molecular mechanism underlying pancreas development and a potential treatment source for type 1 diabetes. Previous studies have shown that miR-181c-5p is highly enriched in adult islets; however, its role in pancreatic β cell differentiation is poorly understood. In this study, we differentiated human induced pluripotent stem cells (hiPSCs) into IPCs in a stepwise process that recapitulated pancreas organogenesis and observed that miR-181c-5p continuously accumulated throughout the entire differentiation process. hiPSCs were transduced with lentiviral vectors containing human miR-181c-5p precursor, which significantly increased the endodermal markers SOX17, FOXA2, CXCR4 and GATA4 and pancreatic endocrine-specific gene expression, including PDX1, NKX6.1, MAFA and Insulin. miR-181c-5p overexpression exerted little effect on the efficiency of definitive endoderm, whereas it promoted the differentiation of pancreatic progenitors and IPCs, especially for NKX6.1-positive and insulin-positive cells differentiation. Transplanted these cells exhibit glucose-stimulated C-peptide secretion in vivo and protect mice from chemically induced diabetes. It was found that miR-181c-5p directly targets the 3′UTR of smad7 and TGIF2 mRNA, which are known to be endogenous repressors of TGF-β-smad2/3 signaling, to decrease their mRNA and protein levels. Furthermore, overexpressed miR-181c-5p led to an elevation of the smad2/3 phosphorylation levels in hiPSC-derived cells, while treatment with smad2/3 inhibitors following miR-181c-5p overexpression had opposite effects on IPC formation. These results suggest that miR-181c-5p is critically involved in pancreatic lineage commitment through direct repression of smad7 and TGIF2 and that it modulates TGF-β-smad2/3 signaling activation and increases the feasibility of using patient-specific hiPSCs for β cell replacement therapy for type 1 diabetes.
The pericyte connectome: spatial precision of neurovascular coupling is driven by selective connectivity maps of pericytes and endothelial cells and is disrupted in diabetes
Authors: Kovacs-Oller Tamas , Ivanova Elena , Bianchimano Paola , Sagdullaev Botir T. 
Published: June 2020

AbstractFunctional hyperemia, or the matching of blood flow with activity, directs oxygen and nutrients to regionally firing neurons. The mechanisms responsible for this spatial accuracy remain unclear but are critical for brain function and establish the diagnostic resolution of BOLD-fMRI. Here, we described a mosaic of pericytes, the vasomotor capillary cells in the living retina. We then tested whether this net of pericytes and surrounding neuroglia predicted a connectivity map in response to sensory stimuli. Surprisingly, we found that these connections were not only selective across cell types, but also highly asymmetric spatially. First, pericytes connected predominantly to other neighboring pericytes and endothelial cells, and less to arteriolar smooth muscle cells, and not to surrounding neurons or glia. Second, focal, but not global stimulation evoked a directional vasomotor response by strengthening connections along the feeding vascular branch. This activity required local NO signaling and occurred by means of direct coupling via gap junctions. By contrast, bath application of NO or diabetes, a common microvascular pathology, not only weakened the vascular signaling but also abolished its directionality. We conclude that the exclusivity of neurovascular interactions may thus establish spatial accuracy of blood delivery with the precision of the neuronal receptive field size, and is disrupted early in diabetes.