bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Rice Promoter Protein Binding Microarray for Cis-Acting Elements for Rice Transcription Factors
Authors: KIM JOUNG SUG , Chae SongHwa , Jun Kyong Mi , Lee Gang-Seob , Jeon Jong-Seong , Kim Kyungdo , Kim Yeon-Ki 
Published: May 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Spad Value Varies With Age And Leaf Of Maize Plant And Its Relationship With Grain Yield
Authors: Kandel Bishnu 
Published: May 2020

Abstract
Objectives A field experiment was conducted to evaluate ten hybrid maize for photosynthetic traits. Ten hybrid were replicate thricely under Randomized Complete Block Design (RCBD) during winter season of 2018. SPAD 502 Plus meter was used for SPAD value measurement. Vegetative stage all photosynthetic measurement taken from T1 (top most leaf) and T3 (2nd leaf from top leaf) leaves of 5 plants of one plot during 30 days interval. For reproductive phase data taken from Eo/ eo (Leaf attach to cob/ear), E2 or e2 (2nd leaf from Eo /eo leaf) leaves during entire period of grain filling at 10 days intervals. Same leaves were used for overall data collection.Results Result showed that significantly different SPAD value were observed for different age and leaves of maize during pre and post anthesis. SPAD value increase with increase in age and decrease at the time of maturity. During vegetative and juvenile phase T3 leaf has more SPAD value than T1. During reproductive stage eo leaves had more SPAD than e2 leaves, so center leaf of maize contributes more to grain yield. Correlation studies showed that there is strong positive correlation between different stage of SPAD with grain yield.
Efficacy and safety of a novel personalized navigation template in proximal femoral corrective osteotomy for the treatment of DDH
Authors: shi qiang , Sun Deyi 
Published: May 2020

Abstract
Background This present study is aimed to retrospectively evaluate the efficacy and safety of a novel personalized navigation template in proximal femoral corrective osteotomy for the treatment of DDH.Methods Twenty-nine consecutive patients with DDH who underwent proximal femoral corrective osteotomy were evaluated between August 2013 and June 2017. Based on the different surgical methods, they were divided into the conventional group (n = 14) and navigation template group (n = 15). The osteotomy degrees, radiation exposure, and operation time were compared between the two groups.Results No major complications relating to osteotomy surgery such as redislocation or avascular necrosis occurred in the navigation template group, which had more accurate osteotomy degrees, less radiation exposure, and shorter operation time when compared with the conventional group (P < 0.05). Moreover, there was significant difference according to the McKay criteria between the two groups (P = 0.0362).Conclusions The a novel personalized navigation template in proximal femoral corrective osteotomy is effective and safe, which could improve the femoral osteotomy accuracy, reduce radiation exposure, and shorten operation time.
The Feasibility Of Co-Registered Solid-State 123i-Mibg Spect And Ct Imaging For The Assessment Of Ventricular And Atrial Sympathetic Innervation In Healthy Individuals
Authors: Nikitin Nikita , Minin Stanislav , Stirrup James , Pokushalov Evgeny , Romanov Alexander 
Published: May 2020

Abstract
Background. To evaluate global and regional ventricular and atrial cardiac iodine-123 meta-iodobenzylguanidine (123I-mIBG) uptake and consistency over time in healthy individuals using co-registered single-photon emission computed tomography (SPECT) and computed tomography (CT) imaging. Fifteen healthy individuals (median age 31 years [26; 41]) were included in the study. All participants underwent CT and subsequent baseline 123I -mIBG SPECT imaging (early and late acquisition) using a dedicated cardiac solid-state gamma camera. The heart-to-mediastinum (H/M) ratio, wash out rate (WR), summed 123I-mIBG defect score (SDS) as well as presence and patterns of left atrium (LA) discrete 123I-mIBG uptake areas (DUA) were assessed. Follow-up SPECT imaging was acquired 5–7 days after initial procedure.Results. At baseline median H/M ratio on the early and late acquisitions were 1.61 [1.57; 1.71] and 1.68 [1.65; 1.71] respectively, the WR was 22.5% [18.8; 22.8]. Areas of reduced 123I-mIBG uptake were detected in 60% (9/15) of cases and the median SDS was 1 [0; 2]. No significant changes were observed in global and regional 123I-mIBG cardiac uptake between baseline and follow-up studies. At baseline 36 DUA were identified, 16 (44%) of which (median per individual 1 [1;1]) had moderate-high confidence score (CS). 5/16, 4/16, 4/16 and 3/16 moderate-high CS DUAs were located around the left sided-, right sided pulmonary veins ostia, LA walls or right atrium (RA) respectively. At follow-up 33 DUAs were identified, 16 (48%) of which (median per individual 1 [1;1], p = 0.5 vs baseline) had moderate-high CS. Moderate-high CS discrete uptake areas had generally the same location as on the baseline procedure.Conclusion. Co-registered solid-state 123I-mIBG SPECT and CT is feasible for the evaluation of left ventricle sympathetic innervation as well as for the detection of discrete sympathetic areas in the typical anatomical sites of left atrial ganglionated plexi with modest variations in the constancy of uptake locations. There were no significant changes in the global and regional 123I-mIBG left ventricular uptake over a short time interval in healthy individuals.
Glide Path Enlargement of Curved Molar Canals Using HyFlex EDM Glide Path File versus PathFile: A Comparative Study of Preparation Times and Postoperative Pain
Authors: Han Yi , Hou Xiao-Mei 
Published: May 2020

Abstract
Background This randomized clinical trial aimed to compare the preparation times and severity of postoperative pain between the HyFlex EDM Glide Path File and PathFile. Methods Eighty patients who were treated by the same specialist were enrolled. After access cavity preparation, the patients were randomly assigned to receive glide path enlargement with either HyFlex EDM GPF or PathFile. ProTaper Next X1 and X2 files were used to prepare the canals. The time of preparation was assessed and the severity of postoperative pain over the next 7 days was recorded. Results Preparation time and postoperative pain severity were significantly lower in the HyFlex EDM GPF group compared to that in the PathFile group ( P < 0.05 for both). Conclusion Postoperative pain and glide path preparation time could be reduced by using the HyFlex EDM GPF system.
Comparison of CPR training by social media networks and workshop on CPR skill of nursing and midwifery students
Authors: Ghorbani Sara , Ghafourifard Mansour , Dinmohammadi Mohammadreza , Fallah Ramezan , aghajanloo ali 
Published: May 2020

Abstract
Background: Smartphone-based education has been recommended in different areas of medical education. Considering the capabilities and features of social media, this study aimed to investigate the effect of social media and workshop on CPR performance of nursing and midwifery students. Methods: In this quasi-experimental study, 59 nursing and midwifery students were randomly divided into two groups (WhatsApp- and workshop-based training). Students' CPR skill was assessed at four time-points including before the training, one week, one month and three months after the training. Results: The results showed that the mean score of CPR skill in the workshop group was higher than WhatsApp in one week, one month, and three month later (p<0.05). Conclusions: Despite the effectiveness of both methods, workshop had a better effect on students' performance. Therefore, social media should not be considered a substitute for conventional training such as the workshop.
Gender Disparity in Cardiovascular Mortality following Radiation Therapy for Hodgkin's Lymphoma: A Systematic Review
Authors: Khalid Yaser , Fradley Michael , Dasu Neethi , Dasu Kirti , Shah Ankit , Levine Adam 
Published: May 2020

Abstract
Background Radiation-induced coronary artery disease (R-CAD) has become an increasingly recognized phenomenon. Although the clinical relationship between radiation therapy and CAD risk is well known, there has been very little investigation of the gender relationship to radiation-induced CAD events and resulting cardiovascular (CV) mortality. We study the gender variation in the incidence of CV events/mortality related to R-CAD in Hodgkin’s Lymphoma (HL) patients. Methods The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were used in this systematic review and network meta-analysis. PubMed, google scholar, and Cochrane databases were searched to identify prospective and retrospective observational studies comparing women and men following radiation treatment for Hodgkin's lymphoma. 10 studies were included (4 prospective, 6 retrospective). The primary outcome was incidence of cardiovascular events and mortality. The secondary outcome was all-cause mortality. Meta-regression for age was also performed. Results Of 13,975 patients including 41% females and 59% males, CV events/mortality was noted to be significantly higher in women compared to men (OR 3.74, 95% CI 2.44-5.72, p <0.001). All-cause mortality was also higher in women compared to men (OR 1.94, 95% CI 1.10-3.44, p <0.023). On meta-regression analysis, elderly populations have a higher rate of mortality which was even higher for women than men (coefficient = 0.0458, p=0.0374). Conclusions Women have a higher rate of CAD related CV events/mortality and all-cause mortality compared to men in radiation treated patients. These data highlight the need for increased surveillance to better monitor for CAD in female patients treated with mantle or mediastinal radiation.
Clinical characteristics and risk factors for severe-critically ill COVID-19 adult patients in Jiangsu, China: a multiple-centered, retrospective study
Authors: Zhao Jiangnan , Zhu Meiying , Su Xin , Huang Mao , Yang Yi , Huang Jianan , Songshi Ni , Cao Quan , Gu Qin , Li Jun , Li Jiashu , Zhao Wenjing , Shi Bin , Shi Yi 
Published: May 2020

Abstract
Background A number of reports have documented the clinical characteristics of patients with severe coronavirus disease 2019 (COVID-19) in Wuhan. Clinical features of severe-critically ill COVID-19 patients in Jiangsu, outside Wuhan, remains unknown. Methods This multi-centered retrospective study collected the information of 631 laboratory-confirmed COVID-19 patients hospitalized at 28 authorized hospitals in Jiangsu province between January 23, 2019 and March 13, 2020. Epidemiological and demographic information, clinical and radiological characteristics, laboratory results and treatment of these patients were analyzed. Results A total of 583 adult patients with laboratory-confirmed COVID-19 were enrolled for final analysis, including 84 severe-critically ill patients and 499 mild-moderate patients. Median age of the severe-critically ill patients was 57.0 years [interquartile range (IQR), 49.0-65.8], and 50 (59.5%) were males. Multisystemic laboratory abnormalities were observed on admission in severe-critically ill patients. The severe-critically ill patients showed more noticeable radiologic abnormalities and more coexisting health issues as compared to mild-moderate patients. Most of the severe-critically ill COVID-19 patients become deteriorated in two weeks after diagnosis. Age [odds ratio (OR) 1.08, 95% confidence interval (CI) (1.03-1.14)], D-dimer (OR 3.21, 95% CI 1.39-7.40), and lymphocytes (OR 0.28, 95% CI 0.04-0.88) were independently associated with the progression of severe-critically illness. Conclusions Older age, higher D-dimer levels and less lymphocyte counts on admission are potential risk factors for COVID-19 patients to develop into severe and critically illness. The results would help clinicians to identify high-risk patients in advance.
Synergic effects of decellularized bone matrix, hydroxyapatite, and extracellular vesicles on repairing of the rabbit mandibular bone defect model
Authors: Emami Asrin , Talaei-Khozani Tahereh , Tavanafar Saeid , Zareifard Nehleh , Azarpira Negar , Vojdani Zahra 
Published: May 2020

Abstract
Background: Extracellular vesicles (ECV) and bone extracellular matrix (ECM) have beneficial effects on the treatment of some pathological conditions. The purpose of this study was to find the synergic effects of decellularized bone (DB) ECM and ECVs on the repair of rabbit. Methods: The quality of decellularized sheep bones was confirmed by H&E, Hoechst, DNA quantification, immunohistochemistry, histochemical staining, and scanning electron microscopy (SEM). Osteoblast-derived ECVs were evaluated by internalization test, Transmission electron microscopy, Dynamic light scattering, and flow cytometry for CD9, CD63, CD81 markers. The hydrogel containing DB and hydroxyapatite (HA) with or without ECVs was evaluated for osteoblast functions and bone repair both in vitro and in vivo. Results: The data indicated ECM preservation after decellularization as well as cell depletion. In vitro assessments revealed that mineralization and alkaline phosphatase activity did not improve after treatment of MG63 cells by ECVs, while in vivo morphomatrical estimations showed synergic effects of ECVs and DB+HA hydrogels on increasing the number of bone-specific cells and vessel and bone area compared to the control, DB+HA and ECV-treated groups. Conclusion: The DB enriched with ECVs can be an ideal scaffold for bone tissue engineering and may provide a suitable niche for bone cell migration and differentiation.
Prevalence of human cytomegalovirus in colorectal cancer and viral gene expression profiles
Authors: Cai Zhengzai , ye lele , Zhu Yuesheng , Lin Ying , Guo Gangqiang , Yan Kejing , Wu Yuqing , Yu Xiaoxiao , Ding Ning , Lin Feng , Zhu Shanli , Xue Xiangyang 
Published: May 2020

Abstract
Background Human cytomegalovirus (HCMV) infection plays a crucial role in the development and progression of cancer. However, the effect of HCMV on colorectal cancer (CRC) remains controversial. This study was performed to explore the pathogenesis of HCMV in CRC. Methods HCMV DNA was detected in 74 CRC and paired normal samples by PCR. HCMV IEA protein expression was confirmed in 717 CRC biopsies by immunohistochemistry. HCMV gene expression profiles (GEPs) were further analyzed in 5 CRC tissues by transcriptome sequencing. The associations of HCMV infection with clinical features and prognosis were also evaluated. Results The prevalence rates of HCMV in CRC tissues were 29.73% and 23.17% at the DNA and protein levels respectively, which was significantly higher than those in normal tissues (0%). Transcriptome sequencing to evaluate the GEPs revealed 119 HCMV genes in CRC tissues. The high reads of transcriptions were RNA2.7, RNA4.9, RNA5.0, RL5A, UL82, UL83, and UL70, which correlate with gene expression or regulation. Survival analysis showed that patients with CRC patients and pIEA(++) had longer overall survival (OS) than those with pIEA(+)s at the protein level. However, there was no correlation between pIEA expression and clinical features. Conclusions HCMV, a common virus found in CRC tissues, is related to the development and progression of CRC. GEP analysis revealed genes correlated with lytic infection. Additionally, genes functioning in gene expression or regulation showed high expression in CRC. We found that CRC patients with HCMV lytic infection have a better prognosis than those with non-HCMV infection. Here, we revealed features of the pathogenic mechanism and provide insight that may be useful for targeted treatment of CRC.
Identification of key genes unique to the luminal A and basal-like breast cancer subtypes via bioinformatic analysis
Authors: Jia Rong , Li Zhongxian , Liang Wei , Ji Yucheng , Weng Yujie , Liang Ying , Ning Pengfei 
Published: May 2020

Abstract
Background Breast cancer subtypes are statistically associated with prognosis. The search for markers of breast tumor heterogeneity and the development of precision medicine for patients are the current focuses of the field. Methods We used a bioinformatic approach to identify key disease-causing genes unique to the luminal A and basal-like subtypes of breast cancer. First, we retrieved gene expression data for luminal A breast cancer, basal-like breast cancer, and normal breast tissue samples from The Cancer Genome Atlas database. The differentially expressed genes unique to the 2 breast cancer subtypes were identified and subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. We constructed protein–protein interaction networks of the differentially expressed genes. Finally, we analyzed the key modules of the networks, which we combined with survival data to identify the unique cancer genes associated with each breast cancer subtype. Results We identified 1,114 differentially expressed genes in luminal A breast cancer and 1,042 differentially expressed genes in basal-like breast cancer, of which the subtypes shared 500. We observed 614 and 542 differentially expressed genes unique to luminal A and basal-like breast cancer, respectively. Through enrichment analyses, protein–protein interaction network analysis, and module mining, we identified 8 key differentially expressed genes unique to each subtype. Analysis of the gene expression data in the context of the survival data revealed that high expression of NMUR1 and NCAM1 in luminal A breast cancer statistically correlated with poor prognosis, whereas the low expression levels of CDC7 , KIF18A , STIL , and CKS2 in basal-like breast cancer statistically correlated with poor prognosis. Conclusions NMUR1 and NCAM1 are novel key disease-causing genes for luminal A breast cancer, and STIL is a novel key disease-causing gene for basal-like breast cancer. These genes are potential targets for clinical treatment.
Frailty is a useful predictive marker of postoperative complications after pancreaticoduodenectomy
Authors: NAKANO YUTAKA , Hirata Yuki , Shimogawara Tatsuya , Yamada Toru , Mihara Koki , Nishiyama Ryo , Nishiya Shin , Taniguchi Hideki , Egawa Tomohisa 
Published: May 2020

Abstract
BACKGROUND: Frailty results in a high risk for disability, hospitalization, and mortality. This study aimed to investigate perioperative details of frail patients who underwent pancreatectomy and whether frailty can be a predictive factor of postoperative complications, especially of clinically relevant postoperative pancreatic fistula (CR-POPF). METHODS: This retrospective study included patients who underwent pancreatectomy in our hospital between August 2016 and March 2019. The patients were divided into frail and pre-/non-frail groups. The diagnostic criteria were based on the Japanese version of the Cardiovascular Health Study. RESULTS: Of 93 patients, 11 (11.8%) and 82 (88.2%) were frail and pre-/non-frail patients, with median ages of 82 and 72 years, respectively (p=0.041). Postoperative complications (Clavien-Dindo ≧IIIa) were found in 8 and 32 patients (p=0.034), CR-POPF in 3 and 13 patients (p=0.346), and postoperative hospital stays were 21 and 17 days (p=0.041), respectively. On multivariate analysis, frailty was an independent predictive factor (odds ratio [OR] 5.604, 95.0% confidence interval [CI] 1.002-30.734; p=0.047) of postoperative complications (Clavien-Dindo ≧IIIa) after pancreaticoduodenectomy. On multivariate analysis, a soft pancreas (OR 5.696, 95.0% CI 1.142-28.149; p=0.034) was an independent and significant predictive factor of CR-POPF after pancreaticoduodenectomy. CONCLUSIONS: Frailty may be a useful predictive factor of postoperative complications in patients undergoing pancreaticoduodenectomy.
Angiopoietin-Tie2 and nitric oxide signaling of erythrocytes in response to surgical trauma in head and neck cancer
Authors: Wu Hsiang-Ling , Chu You-Hsiang , Tai Ying-Hsuan , Tsou Mei-Yung , Wu Cheng-Hsien , Lo Wen-Liang , Tai Shyh-Kuan , Yeh Chun-Chang , Lu Chih-Cherng 
Published: May 2020

Abstract
Background Angiopoietin-Tie2 and nitric oxide pathway is crucial in tumor angiogenesis and closely correlates with tumor development, growth, and metastasis. This study aimed to investigate the angiopoietin-Tie2 and nitric oxide signaling of erythrocyte membrane in response to surgical trauma in head and neck cancer.Methods We prospectively enrolled the patients with histology-proven head and neck squamous cell carcinoma undergoing surgical resection of primary tumors at the medical center between August and November 2019. We measured the preoperative and postoperative levels of angiopoietin-1, angiopoietin-2 in plasma using enzyme-linked immunosorbent assays, nitric oxide in plasma using nitrate/nitrite colorimetric assays, and Tie2 phosphorylation in erythrocyte membrane using Western blotting. Results The plasma angiopoietin-1 was down-regulated from median 971.3 pg/mL (interquartile range [IQR]: 532.1 – 1569.3) to 417.9 (IQR: 270.5 – 597.3) after tumor resection ( p =0.0020). Conversely, the plasma angiopoietin-2 was enhanced from 1173.6 pg/mL (IQR: 977.7 – 1450.2) to 2353.7 (IQR: 1352.4 – 2954.3) after surgery ( p =0.0021), with a concomitant increase in plasma nitric oxide level from 7.73 μM (IQR: 5.39 – 10.06) to 10.50 (IQR: 7.65 – 14.18) after surgical resection ( p =0.0093). Subgroup analyses further showed the angiopoietin-Tie2 and nitric oxide signaling was significant only in stage III and IV cancer.Conclusions The dynamic change of angiopoietin-Tie2 signaling in erythrocyte membrane along with the enhanced nitric oxide in plasma after tumor resection suggests erythrocytes play a significant role in modulating surgery-induced angiogenesis, which may provide a novel marker for cancer surveillance and control.
Prevalence of malnutrition and associated risk factors among under-five children in Amhara Region, Ethiopia: evidence from 2016 Ethiopian Demographic and Health Survey
Authors: Mengesha Damitie Kebede , Merkeb Yidnekachew 
Published: May 2020

Abstract
Background Childhood malnutrition is the most widely prevalent among under-five children in Amhara Region, Ethiopia. This study intended to explore the major determinants of malnutrition and its association with anemia among under-five children in Amhara Region, Ethiopia.Methods The data from the 2016 Ethiopian Demographic and Health Survey were used. A total of 977 under-five children were included in this analysis. A multivariable binary logistic regression analysis was used at a 5% level of significance to determine the individual- and community-level factors associated with childhood malnutrition. Results The prevalence of stunting, wasting, and underweight were 46.3%, 9.8%, and 28.4%, respectively. About 23.1% of children were both stunting and underweight, 7.3% were both underweight and wasting, and only 4.5% of children had all the three conditions. Among the factors considered in this study, Age of child in months, size of child at birth, mother highest education level, sex of household head, sources of drinking water, and type of toilet facility were significantly associated with malnutrition in Amhara Region. Conclusion Malnutrition among under-five children was one of the public health problems in the Amhara Region. The influence of these factors should be considered to develop strategies for reducing malnutrition in Amhara Region. Finally improving the living standards of the children is important to get better health care, to enhance the child’s nutritional status, and reduce child mortality.