bluuebunny/crossref_metadata_2025_split huggingface dataset:
Iron Chelator-Mediated Anoxic Biotransformation of Lignin by Novel sp.,Tolumonas lignolyticaBRL6-1
Authors: Chaput Gina , Billings Andrew , DeDiego Lani , Orellana Roberto , Adkins Joshua N , Nicora Carrie , Chu Rosalie , Simmons Blake , DeAngelis Kristen M. 
Published: May 2020

AbstractLignin is the second most abundant carbon polymer on earth and despite having more fuel value than cellulose, it currently is considered a waste byproduct in many industrial lignocellulose applications. Valorization of lignin relies on effective and green methods of delignification, with a growing interest in the use of microbes. Here we investigate the physiology and lignin biotransformation mechanisms of the novel facultative anaerobic bacterium,Tolumonas lignolyticaBRL6-1, under anoxic conditions. Physiological and biochemical changes were compared between cells grown anaerobically in either lignin-amended or unamended conditions. In the presence of lignin, BRL6-1 had a higher biomass and shorter lag phase compared to unamended conditions, and 14% of the proteins determined to be significantly higher in abundance by log2fold-change of 2 or greater were related to Fe(II) transport in early exponential phase. Ferrozine assays of the supernatant (<10 kDa fraction) confirmed that Fe(III) was bound to lignin and reduced to Fe(II) only in the presence of BRL6-1, suggesting redox activity by the cells. LC-MS/MS analysis of the secretome showed an extra band at 20 kDa in lignin-amended conditions. Protein sequencing of this band identified a protein of unknown function with homology to enzymes in the radical SAM superfamily. Expression of this protein in lignin-amended conditions suggests its role in radical formation. From our findings, we suggest that BRL6-1 is using a protein in the radical SAM superfamily to interact with the Fe(III) bound to lignin and reducing it to Fe(II) for cellular use, increasing BRL6-1 yield under lignin-amended conditions. This interaction potentially generates organic free radicals and causes a radical cascade which could modify and depolymerize lignin. Further research should clarify the extent to which this mechanism is similar to previously described aerobic chelator-mediated Fenton chemistry or radical producing lignolytic enzymes, such as lignin peroxidases, but under anoxic conditions.
Microtubule re-organization during female meiosis inC. elegans
Authors: Lantzsch Ina , Yu Che-Hang , Chen Yu-Zen , Zimyanin Vitaly , Yazdkhasti Hossein , Lindow Norbert , Szentgyörgyi Erik , Pani Ariel , Prohaska Steffen , Srayko Martin , Fürthauer Sebastian , Redemann Stefanie 
Published: May 2020

AbstractThe female meiotic spindles of most animals are acentrosomal and undergo striking morphological changes while transitioning from metaphase to anaphase. The ultra-structure of acentrosomal spindles, and how changes to this structure correlate with such dramatic spindle rearrangements remains largely unknown.To address this, we applied light microscopy, large-scale electron tomography and mathematical modeling of female meioticC. elegansspindles undergoing the transition from metaphase to anaphase. Combining these approaches, we find that meiotic spindles are dynamic arrays of short microtubules that turn over on second time scales. The results show that the transition from metaphase to anaphase correlates with an increase in the number of microtubules and a decrease in their average length. Detailed analysis of the tomographic data revealed that the length of microtubules changes significantly during the metaphase-to-anaphase transition. This effect is most pronounced for those microtubules located within 150 nm of the chromosome surface. To understand the mechanisms that drive this transition, we developed a mathematical model for the microtubule length distribution that considers microtubule growth, catastrophe, and severing. Using Bayesian inference to compare model predictions and data, we find that microtubule turn-over is the major driver of the observed large-scale reorganizations. Our data suggest that in metaphase only a minor fraction of microtubules, those that are closest to the chromosomes, are severed. The large majority of microtubules, which are not in close contact with chromosomes, do not undergo severing. Instead, their length distribution is fully explained by growth and catastrophe alone. In anaphase, even microtubules close to the chromosomes show no signs of cutting. This suggests that the most prominent drivers of spindle rearrangements from metaphase to anaphase are changes in nucleation and catastrophe rate. In addition, we provide evidence that microtubule severing is dependent on the presence of katanin.
Spatio-temporal patterning of living cells with extracellular DNA programs
Authors: Van Der Hofstadt Marc , Galas Jean-Christophe , Estevez-Torres André 
Published: May 2020

AbstractReactive extracellular media focus on engineering reaction networks outside the cell to control intracellular chemical composition across time and space. However, current implementations lack the feedback loops and out-of-equilibrium molecular dynamics for encoding spatio-temporal control. Here, we demonstrate that enzyme-DNA molecular programs combining these qualities are functional in an extracellular medium where human cells can grow. With this approach, we construct an internalization program that delivers fluorescent DNA inside living cells and remains functional for at least 48 h. Its non-equilibrium dynamics allows us to control both the time and position of cell internalization. In particular, a spatially inhomogeneous version of this program generates a tunable reaction-diffusion two-band pattern of cell internalization. This demonstrates that a synthetic extracellular program can provide temporal and positional information to living cells, emulating archetypal mechanisms observed during embryo development. We foresee that non-equilibrium reactive extracellular media could be advantageously applied toin vitrobiomolecular tracking, tissue engineering or smart bandages.
Cellular taxonomy and spatial organization of the ventral posterior hypothalamus reveals neuroanatomical parcellation of the mammillary bodies
Authors: Mickelsen Laura E. , Flynn William F. , Springer Kristen , Wilson Lydia , Beltrami Eric J. , Bolisetty Mohan , Robson Paul , Jackson Alexander C. 
Published: May 2020

ABSTRACTThe ventral posterior hypothalamus (VPH) is an anatomically complex brain region implicated in arousal, reproduction, energy balance and memory processing. However, neuronal cell type diversity within the VPH is poorly understood, an impediment to deconstructing the roles of distinct VPH circuits in physiology and behavior. To address this question, we employed a droplet-based single cell RNA sequencing (scRNA-seq) approach to systematically classify molecularly distinct cell types in the mouse VPH. Analysis of >16,000 single cells revealed 20 neuronal and 18 non-neuronal cell populations, defined by suites of discriminatory markers. We validated differentially expressed genes in a selection of neuronal populations through fluorescencein situhybridization (FISH). Focusing on the mammillary bodies (MB), we discovered transcriptionally-distinct clusters that exhibit a surprising degree of segregation within neuroanatomical subdivisions of the MB, while genetically-defined MB cell types project topographically to the anterior thalamus. This single cell transcriptomic atlas of cell types in the VPH provides a detailed resource for interrogating the circuit-level mechanisms underlying the diverse functions of VPH circuits in health and disease.
An innovation management assessment framework
Authors: Lobo Stanislaus , Samaranayake Premaratne 
Published: May 2020

PurposeThis paper proposes an integrated approach for assessing innovation management practices using an innovation management assessment framework, with the application of the innovation management assessment framework at the incubation and firm level.Design/methodology/approachInnovation management assessment framework is developed, based on the literature review. An innovation management assessment framework toolkit for training innovation practitioners is proposed as the basis for the framework implementation in the industry. The main approach is to make close alignment of the design for lean six sigma phases within broader innovation and stage-gate model in the innovation management assessment framework.FindingsThe operationalization of the innovation management assessment framework is enabled and supported by an assessment framework and a toolkit for managing innovation.Practical implicationsA roadmap for innovation management and assessment of performance at incubatee/firm level.Social implicationsIt provides a tool for developing innovative products and services for incubatees in start-ups, thus fueling the economy. It also has applications for ongoing established businesses.Originality/valueInnovation of new business development can be made through a well-designed and implemented innovation programme that is aligned with stage-gate and design for lean six sigma phases through the innovation management assessment framework.
The significance of grassroots and inclusive innovation in harnessing social entrepreneurship and urban regeneration
Authors: Duarte Alonso Abel , Kok Seng Kiat , O'Brien Seamus , O'Shea Michelle 
Published: May 2020

Purpose
The purpose of this study is to examine the dimensions of inclusive and grassroots innovations operationalised by a social enterprise and the impact of these activities on urban regeneration. To this end, the case of Homebaked in Liverpool, UK, is presented and discussed.

Design/methodology/approach
Face-to-face interviews with members of Homebaked’s management, staff and volunteers were conducted; the interviews were complemented with on-site observations and review of archival information of the social enterprise.

Findings
The data gathered revealed the organisation’s involvement in both types of innovation as a means to achieve long-term urban regeneration related goals. For instance, innovative, strategic and human dimensions, together with the human dimension emerged as key ways of innovating. The impacts of innovative practices comprised encouraging inclusiveness among residents and non-residents, with approaches including hands-on training workshops, job and volunteering opportunities being predominant.

Originality/value
First, the study advances the theoretical and applied understanding of grassroots and inclusive innovation in the context of a social enterprise. For instance, an innovative/strategic and human dimension emerged as predominant ways in which grassroots and inclusive innovation elements were manifested. These dimensions were based on technology uptake, implementation of new product/service concepts or harnessing the skills of local and non-local individuals. Similarly, four dimensions associated with the impacts of these types of innovation were revealed. Second, the study addresses acknowledged gaps in the literature, particularly regarding the limited contributions illuminating processes and determinants of innovation among social enterprises.
Investigating the barriers to building information modeling (BIM) implementation within the Nigerian construction industry
Authors: Olanrewaju Oludolapo Ibrahim , Chileshe Nicholas , Babarinde Sunday Ajiboye , Sandanayake Malindu 
Published: May 2020

PurposeThe purpose of this paper is to identify and assess the perceptions of constructional professionals on barriers to implementation of building information modeling (BIM) within the Nigerian construction industry.Design/methodology/approachA scoping literature review was conducted to identify the fourteen barriers to implementation of BIM, which were employed to design a questionnaire survey. Data collected were analyzed using descriptive statistics, mean score, Kruskal–Wallis test, analysis of variance and multivariate techniques such as factor analysis.FindingsThe descriptive and empirical analysis demonstrated a disparity of ranking of the 14 barriers factors among the groups; however no statistically significant differences among the 14 barriers to BIM. Based on the mean score ranking results, only three (out of 14) barriers are identified as critical (mean score greater than 3.5): few studies available on BIM and lack of knowledge, inexistence or inadequate government policies, and high cost of implementation. The results of the one-sample t-tests show that they were statistically significant differences in 10 out of 14 barriers as follows: few studies available on BIM and lack of knowledge, lack of demand for use and acceptance of BIM, inadequate contractual coordination, lack of specified standards, cost of data and information sharing, technological availability issues, reluctance of other stakeholders, business and cultural changes, data and intellectual property issues, and interoperability issues. The study, through factor analysis, categorized the fourteen barriers to BIM implementation into four principal factors. The factors are: technology and business-related barriers; training and people-related barriers; cost and standards-related barriers; and process and economic-related barriers.Practical implicationsThe identification and assessment of the key barriers to BIM implementation would be useful for the construction professionals and other stakeholder of the construction industry with the view to advance BIM adoption in Nigeria. This could also be extended to other developing countries through considerations of the local economic conditions, given the status of BIM as being in the germinating stage of development in Africa.Originality/valueThe study provides insights on the barriers to BIM implementation across the Nigerian construction sector environments. The innovative aspect of the study is the identification of the ordered and grouped (composite) set of barriers to BIM which could be used to developing appropriate mitigating solutions.
Forty years of Employee Relations – The International Journal: a bibliometric overview
Authors: Kataria Aakanksha , Kumar Satish , Sureka Riya , Gupta Bindu 
Published: May 2020

PurposeThis paper examines the leading trends for the journal Employee Relations from 1979 to 2018 upon its completion of 40 years of publication. Through conducting a bibliometric analysis, the article presents the journal's publication and citation structure, prominent themes, significant author keywords and leading articles, authors, institutions and countries since its formation.Design/methodology/approachThe article focuses on the journal's evolution and subsequent growth patterns during this period. Using the Scopus database, the leading trends in authorship, institutions, countries as well as cited documents along with the articles citing it were analysed to provide an analytic overview of the journal over the period of 1979–2018. The paper presents a graphical visualization of the bibliographic data with bibliographic coupling and co-citation analysis using the visualization tools of similarity viewer software as well.FindingsThe results indicate that the journal is on a progressive trend both in terms of productivity as well as the level of influence in the areas for which it is indexed. The journal receives the maximum influence from the UK, including its most productive authors and institutions. The journal has published research on prominent topics in human resource management, employee relations and the field of industrial relations. It has also published 25 special issues on recent trends in the domain of Human Resource (HR).Originality/valueThis article offers the first comprehensive evaluation of the intellectual structure and research contributions of the journal over its lifespan. The findings of the article are useful for researchers and HR practitioners.
Structural breaks in the central government taxes in India, 1950-1951 to 2013-2014
Authors: Rath Anita 
Published: May 2020

Purpose
The purpose of this paper is to find out the factors contributing to major shifts in the growth of tax revenue through the estimation of structural breaks and analysis of major tax regimes. Recent contributions to optimal tax theory and empirical literature on the Laffer curve effect, based on elasticity of taxable income, challenge the settled understanding on the rate-revenue relationship. In this backdrop, the objective of the paper is to find out the relative significance of changes in tax rate, tax base and administrative reforms in affecting the growth of tax revenue in India. The paper considers tax data spanning a period of six and half decades for five major components of direct and indirect taxes (corporation, personal income, customs, excise and service) of the central government of India.

Design/methodology/approach
Unknown break point(s) – single and multiple – in the tax structure are identified by using the Quandt-Andrews and Bai-Perron econometric tests. These tests were conducted for two models of growth of taxes (tax revenue and tax-NDP ratio) estimated using semi-log functions. A simulation exercise was conducted to find out the robustness of the results by varying the trimming parameter and number of breaks. An analytical framework is used to understand the factors associated with these breaks.

Findings
There is more than one break identified for every tax component as per the results of Bai–Perron test. The simulation exercise suggests that estimated breakpoints are mostly robust. Economic growth, structural changes in the economy, simplification and rationalization of tax structure, tax competition, policies such as liberalization have contributed to the changing tax regimes. Results of this study suggest that high tax rates have not been, in particular, detrimental to achieving growth in revenue and factors other than changes in tax rates have been more prominent in bringing about the shifts.

Originality/value
This is, perhaps, the first paper exploring the multiple structural breaks in the fiscal variables in India. It offers an understanding of the changing regimes of central government taxes and the underlying factors for the same.
Forty years review of upstream documents of the Islamic Republic of Iran's health sector on strategic purchasing of advanced-expensive medical equipment
Authors: Bastani Peivand , Tahernezhad Ali , Hakimzadeh Seyyed Mostafa 
Published: May 2020

PurposeAccording to the importance of strategic purchasing as an effective tool for resource allocation and service procurement, this study examines national laws, regulations and other related documents related to the strategic purchasing of health services related to the advanced medical equipment in Iran.Design/methodolgy/approachIt was a national qualitative document analysis conducted in 2019 applying content analysis approach. The four-step Scott method was used to include the documents in terms of authenticity, credibility, representation and meaningfulness. After retrieving the related documents, they were coded with the implicit and explicit approach. MAXQDA10 was used for content analysis.FindingsThe findings show that according to the framework of effective factors on the strategic purchasing of health services, seven main factors are determined as the main essential factors in purchasing advanced medical equipment. These factors consist of health care providers, health service buyers, purchaser and service provider contracts, payment mechanisms, organization and management evaluation of health technology including expensive medical equipment and technology-related.Research limitation/implicationThe study had some limitations as follows: the proposed method should be tested and its feasibility has to be investigated through appropriate tools for Iranian insurance companies and those with the similar settings.Practical implicationThe results of this study can shed more light for policy makers affiliated in Ministry of Health as the main service provider, Ministry of Welfare and the insurance agencies as the main purchasers of health services on paying attention to these seven main themes extracted from the upstream documents and laws and regulations of the Islamic Republic of Iran.Social implicationThe strategic purchasing of expensive high technology-based medical equipment is a necessity for Iranian public health insurance organizations that is emphasized in national documents in the way of implementing this necessity.Originality/valueThis study examines all the laws and regulations and all related documents in the strategic purchasing of health services related to advanced medical equipment, giving an analysis of the most important challenges and requirements of implementing strategic purchasing in the health services provision sector with expensive medical equipment.
Obstacle-avoiding intelligent algorithm for quad wheel robot path navigation
Authors: Mukherjee Subhradip , Kumar R. , Borah Siddhanta 
Published: May 2020

PurposeThe purpose of this work is to propose quad wheel robot with path navigation using an intelligent novel algorithm named as obstacle-avoiding intelligent algorithm (OAIA).Design/methodology/approachThe paper proposes OAIA algorithm, which is used to minimize the path distance and elapsed time between source and goal.FindingsThe hardware implementation of the Quad Wheel Robot design includes a global positioning system (GPS) module for path navigation. An ultrasonic module (HC SR04) is mainly used as the sensing unit for the system. In the proposed scheme, the GPS locator (L80) is used to obtain the current location of the robot, and the ultrasonic sensor is utilized to avoid the obstacles. An ARM processor serves as the heart of the Quad Wheel Robot.Practical implicationsThis paper includes real-time implementation of quad wheel robot for various coordinate values, and the movement of the robot is captured and analysed.Originality/valueThe proposed OAIA is capable of estimating the mobile robot position exactly under ideal circumstances. Simulation and hardware implementation are carried out to evaluate the performance of the proposed system.
Towards the creation of a searchable 3D smart city model
Authors: Lafioune Nawel , St-Jacques Michèle 
Published: May 2020

PurposeThis paper aims to create a new searchable 3D city model to help managers improve their decision-making.Design/methodology/approachThis paper identifies data management basics and the key elements used in the new model design; it further analyzes five-city models, presents its findings and proposes analytical trends for the new model. It discusses the concepts underlying existing models, explains the benefit brought by the proposed model and demonstrates its robustness.FindingsCity systems can be interconnected, thanks to data digitization and the integration of new technologies into different management processes. Although there are several 3D city models available, none of those identified in this research can be queried for several sectors.Research limitations/implicationsThis model design can only be successfully realized in the presence of a public mandate. Potential limitations include information security risks and political non-acceptance.Originality/valueThe present work proposes a searchable and high performance model having the distinctive capacity to bring together city systems and perform real-time data analysis in order to extract important information needed to guide the city, and in the context of a global vision.