bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anthocyanin Fruit encodes an R2R3‐MYB transcription factor, SlAN2‐like, activating the transcription of SlMYBATV to fine‐tune anthocyanin content in tomato fruit
Authors: Yan Shuangshuang , Chen Na , Huang Zejun , Li Dongjing , Zhi Junjie , Yu Bingwei , Liu Xiaoxi , Cao Bihao , Qiu Zhengkun 
Published: October 2019

Summary

Anthocyanin fruit (Aft) and atroviolacea (atv) were characterized in wild tomato and can enhance anthocyanin content in tomato fruit. However, the gene underlying the Aft locus and the mechanism by which Aft and atv act remain largely unknown.

In this study, the Aft locus was fine‐mapped to an approximately 145‐kb interval on chromosome 10, excluding SlAN2 (Solyc10g086250), SlANT1 (Solyc10g086260) and SlANT1‐like (Solyc10g086270), which have previously been suggested as candidates. Thus, the R2R3‐MYB transcription factor SlAN2‐like (Solyc10g086290) was considered the best candidate gene for Aft.

The CRISPR/Cas9‐mediated SlAN2‐like mutants show a much lower accumulation of anthocyanins associated with the downregulation of multiple anthocyanin‐related genes compared to the wild‐type tomato, indicating that SlAN2‐like is responsible for the Aft phenotype. The repressive function of SlMYBATV also was confirmed through the CRISPR/Cas9 approach. A yeast‐two‐hybrid assay revealed that SlMYBATV interacts with the bHLH protein SlJAF13. Furthermore, yeast‐one‐hybrid and dual‐luciferase transient expression assays showed that Aft directly binds to the SlMYBATV promoter and activates its expression.

The results herein provide candidate genes to enhance anthocyanin content in tomato fruit. This research also provides insight into a mechanism involving the Aft‐SlMYBATV pathway that fine‐tunes anthocyanin accumulation in tomato fruit.
The Medtronic Sprint Fidelis® lead history revisited—Extended follow‐up of passive leads
Authors: Frey Simon Martin , Sticherling Christian , Altmann David , Brenner Roman , Kühne Michael , Ammann Peter , Coslovsky Michael , Osswald Stefan , Schaer Beat 
Published: October 2019

AbstractBackgroundDue to high failure rates, Medtronic withdrew the Sprint Fidelis lead (SFL) from the market. Passive fixation lead models exhibited better survival than active models, but most studies have limited follow‐up. Aim of this study was to give insights into passive lead survival with a follow‐up of 10 years.MethodsIn two large Swiss centers, patients with passive SFLs were identified and data from routine implantable cardioverter defibrillator (ICD) follow‐ups were collected. Patients were censored at time of death, last device interrogation (if lost to follow‐up), time of lead revision (in non‐SFL‐related problems), or at database closure (31th December 2017). We defined lead failure as any of the following: lead fracture with inappropriate discharge; sudden increase in low‐voltage impedance to >1500 or high‐voltage impedance to >100 Ω; >300 nonphysiological short VV‐intervals.ResultsWe identified 145 patients. Age at implant was 60 ± 12 years with a median follow‐up of 10.2 (interquartile range [IQR]: 5.0‐11.2) years. Thirty‐five percent of patients died after 5.4 ± 2.7 years. A total of 19 leads (13%) failed after 6.7 ± 3.2 years (range: 1.2‐12.0). Overt malfunction with shocks existed in four patients (3%). Cumulative lead survival was 93.1% at 6, 88.2% at 8, 83.8% at 10, and 77.6% at 11 years, respectively, with 35% of implanted leads under monitoring at 10 years. Lead survival fits best a Weibull distribution with accelerating failure rates (k = 1.95, 95% CI 1.32‐2.87, P < 0.001).ConclusionsDuring very long‐term follow‐up, failure rate of the passive SFL shows an increase resulting in an impaired lead survival of 84% at 10 years.
Patients With Fibromyalgia Reporting Severe Pain but Low Impact of the Syndrome: Clinical and Pain‐Related Cognitive Features
Authors: Angarita‐Osorio Natalia , Pérez‐Aranda Adrián , Feliu‐Soler Albert , Andrés‐Rodríguez Laura , Borràs Xavier , Suso‐Ribera Carlos , Slim Mahmoud , Herrera‐Mercadal Paola , Fernández‐Vergel Rita , Blanco Mª Elena , Luciano Juan V. 
Published: October 2019

AbstractBackgroundFibromyalgia (FM) is a prevalent and highly disabling chronic pain syndrome. However, differences among patients regarding how pain impacts on daily life are remarkable. The main aim of this study was to identify clinical and pain‐related cognitive variables characterizing patients reporting high adaptability despite experiencing severe chronic pain.MethodsTwo hundred and eighty‐three Spanish patients with FM with high levels of pain were classified into 2 groups: (1) those reporting low impact of the syndrome, and (2) those with moderate‐to‐high impact. Perceived stress, anxiety, and depressive symptoms along with pain catastrophizing, psychological inflexibility, and perceived control over pain were evaluated. Differences in sociodemographics, years with FM, past/current major depressive disorder comorbidity, and health‐related economic costs (ie, medications, use of medical services, lost productivity due to sick leave) were also assessed. Stepwise logistic regression analyses predicting group membership from clinical variables and pain‐related cognitive processes as predictors were performed.ResultsLower stress, anxiety, and depressive symptoms, along with reduced pain catastrophism, psychological inflexibility, and perceived control over pain, were found in the low‐impact group. Significant predictors of group membership (low‐impact vs. moderate‐to‐high impact) in regression analyses were “cognitive fusion” (psychological inflexibility), “helplessness” (pain catastrophizing), and depressive symptomatology, together with pain intensity and other FM symptoms.ConclusionsThe present study provides further evidence on resilience resources in chronic pain by identifying some variables (ie, reduced depressive symptomatology, pain catastrophizing, and psychological inflexibility) differentially characterizing a profile of patients with FM who are especially able to adapt to high levels of pain.
Psychological resilience to suicidal thoughts and behaviours in people with schizophrenia diagnoses : A systematic literature review
Authors: Harris Kamelia , Haddock Gillian , Peters Sarah , Gooding Patricia 
Published: October 2019

PurposeSuicide deaths are a major concern in people with schizophrenia diagnoses. However, many people with such diagnoses do not attempt suicide, nor die by suicide, suggesting that some individuals are resilient to the impact of suicide triggers. This systematic literature review aimed to (1) appraise the evidence for psychological factors which confer resilience to suicidal thoughts and behaviours, and (2) categorize these psychological factors into broader psychological constructs which characterize resilience.MethodsThe review was conducted in accordance with the PRISMA guidelines for the reporting of systematic reviews. A literature search of four electronic databases (Web of Science, PubMed, PsycINFO, and MEDLINE) was conducted. A quality evaluation of the included studies was carried out by two independent researchers using a quality assessment tool.ResultsPsychological factors from 27 studies were categorized into four constructs: (1) perceived social support, (2) holding religious and spiritual beliefs, (3) identifying reasons for living, and (4) perceived positive personal skills and attributes.ConclusionsThe limited literature showed that resilience is important in understanding suicidal thoughts and behaviours in people with schizophrenia diagnoses. There is a need for prospective research that investigates moderating effects of psychological resilience in the pathways to suicidal thoughts and behaviours in people with schizophrenia diagnoses.Practitioner points

Novel evidence for four psychological constructs which may confer resilience to suicidal thoughts and behaviours in people with schizophrenia diagnoses.

Strong evidence for the impact of perceived social support and appraisals of personal skills and attributes on the severity of suicidal experiences in people with schizophrenia diagnoses.

There was equivocal evidence for the effect of holding religious and spiritual beliefs on suicide attempts.

Clinical practice would benefit from assessing perceived personal attributes and levels of social support from significant others and health professionals.
Pattern of inheritance of a self‐fertility gene in an autotetraploid perennial ryegrass (Lolium perenne) population
Authors: Do Canto Javier , Studer Bruno , Frei Ursula , Lübberstedt Thomas 
Published: October 2019

AbstractA mutation causing self‐fertility (SF) in perennial ryegrass was studied at the tetraploid level. The aim of this work was to determine a) whether SF remains functional in a tetraploid population and b) whether the SF mutation expresses dominance in heterozygous pollen grains. A tetraploidized plant carrying SF alleles was self‐pollinated to create a segregating F2 population. In the F2 individuals, pollen compatibility ranged between 38% and 84% showing that SF remained functional. The SF locus genotype was the main determinant of pollen compatibility explaining 78% of the variation. The observed segregation was significantly different from the expected under both SF being dominant or recessive models (P(χ2)≤0.001) and tended to be intermediate between them, indicating partial dominance or additive gene action. The frequency of the different genotypes suggested that pollen grains homozygous for the mutation have a competitive advantage over heterozygous pollen and that pollen compatibility is affected by the interaction with additional loci. The implications of our results for breeding polyploid grasses are discussed.
Acute ulceronecrotic adverse reaction to potassium hydroxide 5% solution in the treatment of molluscum contagiosum
Authors: Bresesti Ilia , Ciolfi Christian , Rotatore Giuseppe , Borisov Angel N. , Zuccotti Gian Vincenzo , Brazzelli Valeria 
Published: October 2019

AbstractMolluscum contagiosum is a common childhood condition, and although it is self‐limited, treatments are often prescribed. Several medications are available, but there is no consensus regarding the optimal choice in the pediatric population. We report a child who underwent potassium hydroxide 5% treatment resulting in superficial diffuse erosions caused by the inappropriate application. This underlines the importance of parent education before use of this medication with well‐known caustic properties.
Supplementation with exogenous coenzyme Q10 to media for in vitro maturation and embryo culture fails to promote the developmental competence of porcine embryos
Authors: Maside Carolina , Martinez Cristina A. , Cambra Josep M. , Lucas Xiomara , Martinez Emilio A. , Gil María Antonia , Rodriguez‐Martinez Heriberto , Parrilla Inmaculada , Cuello Cristina 
Published: October 2019

AbstractThe coenzyme Q10 (CoQ10) is a potent antioxidant with critical protection role against cell oxidative stress, caused by the mitochondrial dysfunction. This study evaluated the effects of CoQ10 supplementation to in vitro maturation (IVM) or embryo culture media on the maturation, fertilization and subsequent embryonic development of pig oocytes and embryos. Maturation (Experiment 1) or embryo culture (Experiment 2) media were supplemented with 0 (control), 10, 25, 50 and 100 μM CoQ10. The addition of 10–50 μM CoQ10 to the IVM medium did not affect the percentage of MII oocytes nor the fertilization or the parameters of subsequent embryonic development. Exogenous CoQ10 in the culture medium neither did affect the development to the 2–4‐cell stage nor rates of blastocyst formation. Moreover, the highest concentration of CoQ10 (100 μM) in the maturation medium negatively affected blastocyst rates. In conclusion, exogenous CoQ10 supplementation of maturation or embryo culture media failed to improve the outcomes of our in vitro embryo production system and its use as an exogenous antioxidant should not be encouraged.
Assessment of a germplasm bank for the autochthonous cattle breed Asturiana de la Montaña: Extender (Biociphos vs. BIOXCell) affected sperm quality but not field fertility
Authors: Tamargo Carolina , Hidalgo Carlos O. , Caamaño Jose Néstor , Salman Amer , Fueyo Carmen , Arija Cristina , Fernández Ángel , Merino María J. , Martínez‐Pastor Felipe 
Published: October 2019

AbstractSemen banking is critical to preserving rare and autochthonous breeds. However, protocols can change with time, leaving heterogeneous semen batches. The objective of this study was to assess differences in sperm quality and field fertility. We report differences between batches frozen with the Biociphos and BIOXCell extenders in the Asturiana de la Montaña cryobank (autochthonous and endangered breed, Northern Spain). Doses from 48 bulls were analysed by CASA and flow cytometry. The 85‐days non‐return rates from AI records were used to assess the fertility of 23,853 AI. BIOXCell showed higher quality post‐thawing. Differences increased after a 5‐hr incubation at 37°C, and Biociphos yielded doses with lower resilience. Field fertility did not differ between extenders (Biociphos: 57.4% ± 1.2; BIOXCell: 56.6% ± 3.0), possibly because of AI protocols compensating for differences in quality. However, this needs to be confirmed by controlled intervention studies. In conclusion, batches frozen with Biociphos may require specific strategies for compensating for the lower sperm quality. Regular surveys and evaluation of cryobank procedures may be useful to characterizing stored batches and defining strategies to guaranteeing success in their future use.
Abortion in dairy cattle with advanced twin pregnancies: Incidence and timing
Authors: Garcia‐Ispierto Irina , López‐Gatius Fernando 
Published: October 2019

AbstractThis study examines incidences and timing of abortion in Holstein Friesian dairy cows with advanced twin pregnancies in north‐eastern Spain. The study population consisted of 1,194 twin pregnancies in two herds recorded from 2010 to 2015: 522 bilateral and 672 unilateral. The presence of live twins was determined by transrectal ultrasonography between 55 and 61 days post‐AI and pregnancy confirmed 60 days later. Abortion was recorded in 278 (23.3%) cows before Day 260 of pregnancy: 7 (1.3%) in bilateral and 271 (40.3%) in unilateral twin pregnancies. Using binary logistic regression procedures the following were excluded as risk factors for abortion: herd, year, heat stress (temperature‐humidity index values >72), lactation number and right versus left side for unilateral twins. Based on odds ratios, cows carrying unilateral twins were 49.9 (right unilateral twins) and 49.3 (left unilateral twins) times more likely to suffer abortion than cows with bilateral twins. The average time of abortion for unilateral twins was 173 ± 32 days and ranged from 135 to 249 days, 43% of abortions occurring between 135 and 154 days of gestation. The authors suggest prevention or reduction of unilateral twin pregnancies by follicular puncture and drainage of subordinate follicles at AI or manual rupture of the amniotic vesicle of one of the two twins at the time of pregnancy diagnosis.
Conceptualism and Concept Acquisition
Authors: McAllister Blake 
Published: October 2019

AbstractMany think that the perceptual theory known as “conceptualism” cannot honour a common and intuitive constraint on concept acquisition – that we gain the initial power to deploy primitive concepts through experience. Their argument is: if experience involves the deployment of concepts, then one must possess the power to deploy those concepts prior to experience. I argue that the plausibility of this argument rests on a subtle equivocation. It is true that conceptualism requires a particular kind of power to deploy concepts prior to experience, but not the sort referenced in the intuitive constraint mentioned above. I end by proposing how the conceptualist might satisfy this constraint. I conclude that conceptualism is better situated to account for primitive concept acquisition than typically thought.
Expansin gene loss is a common occurrence during adaptation to an aquatic environment
Authors: Hepler Nathan K. , Bowman Alexa , Carey Robert E. , Cosgrove Daniel J. 
Published: October 2019

SummaryExpansins comprise a superfamily of plant cell wall loosening proteins that can be divided into four individual families (EXPA, EXPB, EXLA and EXLB). Aside from inferred roles in a variety of plant growth and developmental traits, little is known regarding the function of specific expansin clades, for which there are at least 16 in flowering plants (angiosperms); however, there is evidence to suggest that some expansins have cell‐specific functions, in root hair and pollen tube development, for example. Recently, two duckweed genomes have been sequenced (Spirodela polyrhiza strains 7498 and 9509), revealing significantly reduced superfamily sizes. We hypothesized that there would be a correlation between expansin loss and morphological reductions seen among highly adapted aquatic species. In order to provide an answer to this question, we characterized the expansin superfamilies of the greater duckweed Spirodela, the marine eelgrass Zostera marina and the bladderwort Utricularia gibba. We discovered rampant expansin gene and clade loss among the three, including a complete absence of the EXLB family and EXPA‐VII. The most convincing correlation between morphological reduction and expansin loss was seen for Utricularia and Spirodela, which both lack root hairs and the root hair expansin clade EXPA‐X. Contrary to the pattern observed in other species, four Utricularia expansins failed to branch within any clade, suggesting that they may be the result of neofunctionalization. Last, an expansin clade previously discovered only in eudicots was identified in Spirodela, allowing us to conclude that the last common ancestor of monocots and eudicots contained a minimum of 17 expansins.
Bioinspired artificial platelets for transfusion applications in traumatic hemorrhage
Authors: Girish Aditya , Sekhon Ujjal , Sen Gupta Anirban 
Published: October 2019

KEY IDEAS
Among blood components, platelets (PLTs) present the toughest logistic challenges in transfusion due to limited availability, difficult portability and storage, high contamination risks, and very short shelf life (approx. 5 days).
Robust research efforts are being directed to develop biologic PLTs in vitro as well as design biosynthetic and artificial PLT technologies that can potentially resolve these challenges to allow adequate availability and timely transfusion to improve survival in trauma.
A prospective evaluation of oral Yunnan Baiyao therapy on thromboelastographic parameters in apparently healthy cats
Authors: Patlogar Jeffrey E. , Tansey Colleen , Wiebe Melissa , Hybki Gabrielle C. , Trostel Todd , Murphy Lisa A. , Nakamura Reid K. 
Published: October 2019

AbstractObjectiveTo determine the effect of Yunnan Baiyao (YB) on hemostatic parameters measured by thromboelastography (TEG) in apparently healthy cats administered 1 capsule of YB orally twice daily for 1 week.DesignProspective study of client‐owned cats at a small animal specialty hospital.SettingOne private referral center.AnimalsTwenty client‐owned adult cats were prospectively enrolled.InterventionsAll cats underwent echocardiographic examination by the same board‐certified cardiologist to rule out occult cardiomyopathy. Blood samples were collected for analysis of baseline CBC, fibrinogen, and kaolin‐activated TEG values. Cats were administered 1 capsule (250 mg/capsule) of YB twice daily orally for 1 week and the physical examination, CBC, fibrinogen, and TEG were re‐evaluated. Any side effects attributed to YB were noted at this time.Measurements and Main ResultsThree cats were excluded as 2 cats were identified with underlying cardiomyopathy and another cat had a cystic mass in the cranial mediastinum identified via echocardiography. Seventeen cats were treated with YB; however, 1 cat could not complete the study due to severe vomiting associated with YB administration. The remaining 16 cats completed the study, although 2 additional cats experienced transient vomiting. Yunnan Baiyao administration was associated with a significant decrease in HCT and red blood cell count, although no cat became anemic. None of the TEG parameters significantly changed compared to baseline after 1 week of YB therapy.ConclusionsThe results of this study suggest YB at a dose of 1 capsule orally twice daily in cats fails to produce any significant change in hemostatic parameters as measured by TEG, although it did significantly reduce HCT and red blood cell count. Yunnan Baiyao was tolerated for most of the cats, although 3 of 17 (17.6%) cats experienced vomiting. Clinicians should be aware of these effects before considering the use of YB in cats.
Evaluation of pulse oximetry as a surrogate for PaO2in awake dogs breathing room air and anesthetized dogs on mechanical ventilation
Authors: Farrell Kate S. , Hopper Kate , Cagle Laura A. , Epstein Steven E. 
Published: October 2019

AbstractObjectiveTo evaluate the ability of arterial hemoglobin oxygen saturation measurement via pulse oximetry (SpO2) to serve as a surrogate for PaO2in dogs.DesignTwo‐part study: prospective observational and retrospective components.SettingUniversity teaching hospital.AnimalsNinety‐two dogs breathing room air prospectively enrolled on a convenience basis. Retrospective evaluation of 1,033 paired SpO2and PaO2measurements from 62 dogs on mechanical ventilation.InterventionsDogs with concurrent SpO2and PaO2measured on room air had a data sheet completed with blood gas analysis. SpO2, PaO2, and FiO2values were collected from medical records of dogs on mechanical ventilation.Measurements and Main ResultsPredicted PaO2was calculated from SpO2using the dog oxyhemoglobin dissociation curve. The correlation coefficient between measured and predicted PaO2was 0.49 (P < 0.0001) in room air dogs and 0.74 (P < 0.0001) in ventilated dogs. In room air dogs, Bland–Altman analysis between measured minus predicted PaO2versus the average showed a mean bias of −6.0 mm Hg (95% limit of agreement, −35 to 23 mm Hg). The correlation coefficient between PaO2/FiO2and SpO2/FiO2ratios was 0.76 (P < 0.0001). After combining data sets, receiver operating characteristic curve analysis showed the optimal cutoff value for detecting hypoxemia (PaO2 < 80 mm Hg) was an SpO2of 95%, with sensitivity and specificity of 77.8% and 89.5%, respectively. Using this cutoff, 6.9% of SpO2readings failed to detect hypoxemia, whereas 7.2% predicted hypoxemia that was not present.ConclusionsThe SpO2was not clinically suitable as a surrogate for PaO2, though it performed better in mechanically ventilated dogs. As sensitivity for the detection of hypoxemia was poor, pulse oximetry does not appear to be an acceptable screening test. The SpO2/FiO2ratio may have value for evaluation of anesthetized dogs on supplemental oxygen. Arterial blood gas analysis remains ideal for assessment of oxygenation.
Theoretical Study on the Influence of Planet Gear Rim Thickness and Bearing Clearance on Calculated Bearing Life
Authors: Fingerle Andreas , Hochrein Jonas , Otto Michael , Stahl Karsten 
Published: October 2019

Abstract
Planetary gearboxes are becoming more popular due to their high-power density and potentially high efficiency. When the planet bearings are internally mounted, the body of the planet gear has to be hollow. The demand for large outer diameters due to high-load requirements might result in a small planet rim thickness. Depending on the rim thickness, its rigidity may become very low. Due to the low stiffness and the special load conditions caused by the double meshing, the deformation of the planet and its bearings are unique. In this paper, the influence of rim thickness on bearing load and lifetime is examined. The analysis is performed with a finite element method (FEM) model of a planet rim with a built-in cylindrical roller bearing. With the resulting planet deformation from the FEM calculation, the load distribution on the rolling elements in the bearing and the bearing lifetime according to ISO/TS 16281:2008 has been evaluated.