bluuebunny/crossref_metadata_2025_split huggingface dataset:
Technology-based Health Solutions for Cancer Caregivers to Better Shoulder the Impact of COVID-19: A Systematic Review Protocol
Authors: Su Zhaohui , McDonnell Dean , Liang Bin , Kue Jennifer , Li Xiaoshan , Šegalo Sabina , Advani Shailesh , Flores Bertha E , Wang Jing 
Published: September 2020

Abstract
Background Cancer patients are particularly vulnerable to COVID-19, partially owing to their compromised immune systems and curbed or cut cancer healthcare services caused by the pandemic. As a result, cancer caregivers may have to shoulder triple crises: the COVID-19 pandemic, pronounced healthcare needs from the patient, and elevated need for care from within. While technology-based health interventions have the potential to address unique challenges cancer caregivers face amid COVID-19, limited insights are available. Thus, to bridge this gap, we aim to identify technology-based interventions designed for cancer caregivers and report the characteristics and effects of these interventions concerning the distinctive challenges cancer caregivers face amid COVID-19. Additionally, this paper will present practical insights into the diverse intervention approaches that can assist in the delivery of digital health solutions for cancer caregivers amid and beyond COVID-19.Methods A systematic review of the literature will be conducted in PubMed, PsycINFO, CINAHL, and Scopus in September, 2020. Articles that center on technology-based interventions for cancer caregivers will be included in the review. The search strategy was developed in consultation with an academic librarian who is experienced in systematic review studies. Titles, abstracts, and full-text articles will be screened against eligibility criteria developed a priori. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses procedures will be followed for the reporting process.Results NA—This is a protocol study.Conclusions COVID-19 has uprooted cancer care as we know it. Due to barriers introduced by the COVID-19 pandemic, such as medical resources rationing, cancer caregivers often have to step up to address patients' healthcare needs and wants. This, in turn, will exert substantial stress on informal caregivers, above and beyond COVID-19-related burdens the general public shoulders on a daily basis. Findings of this study can shed light on evidence-based and practical solutions cancer caregivers can utilize to mitigate the unique challenges they face amid COVID-19. Furthermore, results of this study will also offer valuable insights for researchers who aim to develop interventions for cancer caregivers in the context of COVID-19. In addition, we also expect to be able to identify areas for improvement that need to be addressed in order for health experts to more adequately help cancer caregivers weather the storm of global health crises like COVID-19 and beyond.Study Protocol Registration: PROSPERO CRD42020196301
MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of Hepatocellular Carcinoma through ERK pathway
Authors: Li Shuangshuang , Shao Jiajia , Lou Guohua , Wu Chao , Liu Yanning , ZHENG MIN 
Published: September 2020

Abstract
Background
Hepatocellular carcinoma (HCC) is one of the most common cancers with high incidence and mortality. But the underlying mechanisms of HCC still remain unclear. Eukaryotic translation initiation factors (eIFs), showing a large effect on tumor development. In this study, we were aimed to investigate the role of eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) in HCC.
Methods
Western blot (WB) in 30 paired HCC tissues and tissue microarrays (TMAs) by immunohistochemistry (IHC) in 89 paired HCC samples were performed to assess EIF4G2 expression. Clone formation, Real-Time Cell Analysis (RTCA), wounding-healing and transwell assays were adopted to evaluate the role of EIF4G2 on HCC cell proliferation, migration and invasion ability. The function of EIF4G2 on HCC tumor growth was conducted by xenograft nude mouse model in vivo. The regulation of miR-144-3p on EIF4G2 was performed by luciferase reporter assay and WB.
Results
EIF4G2 was obviously upregulated in HCC tissues, and high-expression of EIF4G2 was closely related to HCC prognosis. EIF4G2 silencing could inhibit HCC cells growth and metastasis in vitro, and suppress tumorigenesis in vivo by repressing ERK signaling pathway. The results of luciferase reporter assay, WB and IHC staining verified that EIF4G2 was negatively regulated by miR-144. And re-expression of EIF4G2 could partially reverse the inhibiting effect of miR-144 in HCC.
Conclusion
In summary, our study revealed the role of EIF4G2 in HCC development by activating ERK pathway. We also found that EIF4G2 could be negatively regulated by tumor suppressor miR-144. Our investigations indicated that EIF4G2 might be a promising therapeutic target in HCC.
Prognostic Nomogram for Sorafenib Benefit in Hepatitis B Virus related Hepatocellular Carcinoma after Partial Hepatectomy
Authors: Dong Wei , Yan Kai , Yu Hua , Huo Lei , Xian Zhihong , Zhao Yanqing , Li Jutang , Zhang Yuchan , Cao Zhenying , Fu Yong , Cong Wenming , Dong Hui 
Published: September 2020

Abstract
Background The prediction regarding the long-term prognosis of individuals subjected to sorafenib treatment following partial hepatectomy due to hepatitis B virus (HBV) related hepatocellular carcinoma (HCC) is rather difficult.The objective behind this work is to create an effective prognostic nomogram of sorafenib for personalized therapy of HBV-relatedHCC. Methods 234 HBV-related HCC patients subjected or not subjected to sorafenibtreatment following partial hepatectomy at the Eastern Hepatobiliary Surgery Hospital from 2008 to 2013 were compared to investigate the association between index levels and sorafenib benefit. The optimal cut-off point of the overall survival (OS) factor level was determined by x-tile.The selection of indicators is based on clinical findings. The Cox regression model with an interaction term was employed for evaluating the predictive value. Using a multivariate Cox proportional hazards model a Nomogram was subsequently formulated to analyze 113 patients treated with sorafenib. Determination of nomogram’s discriminative ability and predictive accuracy was done using the concordance index (C-index), calibration, and ROC curve. Results Subgroup analysis revealed that Low GPC3,pERK, pAKT, serum AFP levels, without MVI, under 50 years old, male, TNM stage I/II and BCLC stage 0/A wereassociated significantly with a better OS in patients subjected to sorafenib treatment compared to those not treated with sorafenib after surgery. The independent factors for OS were found be GPC3, pERK, pAKT, serum AST and BCLC stage, following multivariate analysis of the sorafenib cohort, which was all included in the nomogram. The survival probability based on the calibration curve shows that the prediction of the nomogram was in good agreement with the actual observation. Survival was predicted to be 0.73(95% CI, 0.67 to 0.78) based on the C-index of the nomogram. The area under the ROC curve (AUC) for the nomogram to predict the survival for 1, 3, and 5-year was 0.726, 0.816, and 0.823, respectively. Conclusion This proposed nomogram has the potential to make quite a precise prediction regarding the prognosis of HBV-related HCC patients,who underwent sorafenib therapy following partial hepatectomy.
A novel humanized Frizzled-7-targeting antibody enhances antitumor effects of Bevacizumab against triple-negative breast cancer via blocking Wnt/β-catenin signaling pathway
Authors: Xie Wei , Zhao Huijie , Wang Fengxian , Wang Yiyun , He Yuan , Wang Tong , Zhang Kunchi , Yang Hao , Zhou Zhaoli , Shi Haibin , Wang Jin , Huang Gang 
Published: September 2020

Abstract
Background
Anti-angiogenic therapy has been widely applied to the clinical treatment of malignant tumors. However, the efficacy of such treatments has been called into question, especially in triple-negative breast cancer (TNBC). Bevacizumab, the first anti-angiogenic agent approved by FDA, actually increases invasive and metastatic properties of TNBC cells, resulting from the activation of Wnt/β-catenin signaling in response to hypoxia. As a critical receptor of Wnt/β-catenin signaling, Frizzled-7 (Fzd7) is aberrantly expressed in TNBC, indicating Fzd7 a potential target for developing drugs to be combined with anti-angiogenic agents.
Methods
Hybridoma technique and antibody humanization technique were utilized to generate a Fzd7-targeting antibody (SHH002-hu1). Biolayer interferometry (BLI) assay and near infrared (NIR) imaging were conducted to detect the affinity and targeting ability of SHH002-hu1. Next, whether SHH002-hu1 could suppress the invasion and migration of TNBC cells induced by Bevacizumab were validated, and the underlying molecular mechanisms were elucidated by luciferase reporter and western blot assays. The nude-mice transplanted TNBC models were established to assess the anti-TNBC activities of SHH002-hu1 when combined with Bevacizumab. Then, the effects on putative TNBC stem-like cells and Wnt/β-catenin signaling were evaluated by immunofluorescence (IF). Further, the tumor-initiating and self-renew capacity of TNBC cells were studied by secondary nude mouse xenograft model and sphere formation assay. In addition, the effects of SHH002-hu1 on the adaptation of TNBC cells to hypoxia were evaluated by the detection of vasculogenic mimicry (VM) and hypoxia-inducible factor-1α (HIF-1α) transcriptional activity.
Results
The novel humanized antibody targeting Fzd7 (SHH002-hu1) exhibited extremely high affinity with Fzd7, and specifically targeted to Fzd7+ cells and tumor tissues. SHH002-hu1 repressed invasion, migration and epithelial-mesenchymal cell transformation (EMT) of TNBC cells induced by Bevacizumab through abating Wnt/β-catenin signaling. SHH002-hu1 significantly enhanced the capacity of Bevacizumab to inhibit the growth of TNBC via reducing the subpopulation of putative TNBC stem-like cells, further attenuating Bevacizumab-enhanced tumor-initiating and self-renew capacity of TNBC cells. Moreover, SHH002-hu1 effectively restrained the adaptation of TNBC cells to hypoxia via disrupting Wnt/β-catenin signaling.
Conclusion
SHH002-hu1 significantly enhances the anti-TNBC capacity of Bevacizumab, and shows the potential of preventing TNBC recurrence, suggesting SHH002-hu1 a good candidate for the synergistic therapy together with Bevacizumab.
Exosomal ANGPTL1 Attenuates CRC Liver Metastasis by Regulating Kupffer Cell Secretion Pattern and Impeding MMP9 induced vascular leakiness.
Authors: Jiang Kai , Chen Haiyan , Fang Yimin , Chen Liubo , Zhong Chenhan , Bu Tongtong , Dai Siqi , Pan Xiang , Fu Dongliang , Qian Yucheng , Wei Jingsun , Ding Kefeng 
Published: September 2020

Abstract
Background: Angiopoietin-like protein 1 (ANGPTL1) has been proved to suppress tumor metastasis in several cancers. However, its extracellular effects on the pre-metastatic niches (PMNs) are still unclear. ANGPTL1 has been identified in exosomes, while its function remains unknown. This study was designed to explore the role of exosomal ANGPTL1 on liver metastasis in colorectal cancer (CRC).Methods: Exosomes were isolated by ultracentrifugation. The ANGPTL1 level was detected in exosomes derived from human CRC tissues. The effects of exosomal ANGPTL1 on CRC liver metastasis were explored by the intrasplenic injection mouse model. The liver PMN was examined by vascular permeability assays. Exosomal ANGPTL1 localization was validated by exosome labeling. The regulatory mechanisms of exosomal ANGPTL1 on Kupffer cells were determined by RNA sequencing. qRT-PCR, Western Blot, and ELISA analysis were conducted to examine gene expressions at mRNA and protein levels.Results: ANGPTL1 protein level was significantly downregulated in the exosomes derived from CRC tumors compared with paired normal tissues. Besides, exosomal ANGPTL1 attenuated liver metastasis and impeded vascular leakiness in the liver PMN. Moreover, exosomal ANGPTL1 were mainly taken up by KCs and regulated the KCs secretion pattern, especially decreasing the MMP9 expression, which finally prevented the liver vascular leakiness. In mechanism, exosomal ANGPTL1 downregulated MMP9 level in KCs by inhibiting the JAK2-STAT3 signaling pathway.Conclusions: Taken together, exosomal ANGPTL1 attenuated CRC liver metastasis and impeded vascular leakiness in the liver PMN by reprogramming the Kupffer cell and decreasing the MMP9 expression. This study suggests a suppression role of exosomal ANGPTL1 on CRC liver metastasis and expands the approach of ANGPTL1 functioning.
Associations Between Muscle Mass, Muscle Morphology and Bone Health in Older Men With Sarcopenia: A Cross-sectional Study
Authors: Mastaviciute Asta , Kilaite Justina , Petroska Donatas , Laurinavičius Arvydas , Tamulaitiene Marija , Alekna Vidmantas 
Published: September 2020

Abstract
Background: The aim of this study was to explore the association between muscle mass, morphology, bone mineral density, and physical function in community-dwelling older men with sarcopenia.Methods: A total of 151 men, 60 years or older were included in this study. Body composition was measured by dual-energy X-ray absorptiometry (DXA). Low bone mineral density was diagnosed if T-score was equal to or below -1.0 SD of mean young men reference range. Sarcopenia was diagnosed according to European Working Group on Sarcopenia in Older People (EWGSOP) criteria: low muscle mass and low muscle strength or low physical performance. Physical performance was evaluated by short physical performance battery. Microbiopsy of musculus vastus lateralis was performed with disposable muscle microbiopsy system. The perimeter and cross-section area of muscle fibers were calculated using image analysis software in whole slide images; type of fiber and their distribution were evaluated as well. Relationship between variables were examined using Spearman’s and Pearson’s correlations. The level of significance (p-value) of < 0.05 was considered as statistically significant.Results: Mean age of the subjects was 72.9 ± 8.02 years. Sarcopenia was diagnosed in 45 (29.8%) men. In the sarcopenia group, 25 muscle biopsies were examined. The average muscle fiber length was 217.47 ± 25.22 microm and average fiber cross-sectional area was 2446 ± 608.87 microm2. In 9 sarcopenic men with T-scores equal or below -2.5, the muscle fiber area had a significant correlation with balance test (r = 0.73, p = 0.025). Multiple significant correlations were found between bone mineral density, lean mass, appendicular lean mass, arm and leg lean mass, gait speed, balance test and handgrip strength.Conclusions: In men with sarcopenia, low lean muscle mass was associated with low femoral neck and hip BMD, and lower muscle strength. In sarcopenic men with osteoporosis, lower muscle fiber area was associated with lower scores of balance test.Trial registration: study protocol has been approved by Lithuanian regional biomedical research ethics committee (No. 158200-03-208-75).
Impact of autologous platelet rich plasma use on postoperative acute kidney injury in type A acute aortic dissection repair: A retrospective cohort analysis
Authors: Tong Jiaqi , Cao Liang , Liu Liwei , Jin Mu 
Published: September 2020

Abstract
Backround: Perioperative coagulopathy and blood transfusion are common in patients undergoing Stanford type A acute aortic dissection (AAD) repair. The autologous platelet-rich plasmapheresis (aPRP) technique is a blood conservation approach to reduce blood transfusions and morbidity in patients at high risk of bleeding. The purpose of this study was to analyze the effect of aPRP on outcomes, especially in postoperative acute kidney injury (post-AKI), in patients undergoing AAD surgery.Methods: Six hundred sixty patients were divided into aPRP and non-aPRP groups according to aPRP use. The primary endpoint was the difference in the incidence of post-AKI between two groups. The secondary endpoints were risk factors for post-AKI and to assess clinical outcomes. The risk factors associated with post-AKI were calculated, and all outcomes were adjusted by propensity-score matching analysis.Results: A total of 272 patients (41.2%) received aPRP, whereas 388 were in the non-aPRP group. Compared to non-aPRP group, the occurrence of post-AKI increased by 14.1% (p=0.002) and 11.1% (p=0.010) with and without propensity adjustment in the aPRP group, respectively. The aPRP group required fewer intraoperative transfusions (p<0.05) and shortened the duration of mechanical ventilation (p<0.05) than those in the non-aPRP group. Multiple regression analyses showed that aPRP (odds ratio: 1.729, 95% confidence interval: 1.225–2.440; p<0.001) was one of the independent risk factors for post-AKI.Conclusions: The use of aPRP significantly reduced intraoperative blood transfusions and decreased postoperative mortality-adjusted mechanical ventilation. However, aPRP use was independently associated with an increased hazard of post-AKI after adjusting for confounding factors.
Development a Set of Scales to Assess Job Satisfaction Among Physicians in Peru: Validity and Reliability Assessment
Authors: Villarreal-Zegarra David , Torres-Puente Roberto , Castillo-Blanco Ronald , Cabieses Baltica , Bellido-Boza Luciana , Mezones-Holguín Edward 
Published: September 2020

Abstract
Background: To assess the evidences of validity and reliability of a set of scales on different areas of job satisfaction (general professional activity, health services management, and working conditions) in Peruvian physicians based on data from the National Survey of Satisfaction of Users in Health (ENSUSALUD).Method: We carried out a psychometric study based on secondary data analysis. Participants were selected from a two-stage probabilistic national representative sampling stratified by political region. Validity was assessed by exploratory and confirmatory factor analysis and measurement invariance analysis. Reliability was assessed by internal consistency coefficients (alpha and omega). The set of scales was composed of items related to three different areas of job satisfaction: 1) satisfaction with general professional activity, 2) satisfaction with the health services management, and 3) satisfaction with the working conditions of the health center.Results: We included 2,137 participants in the analysis. General professional activity scale with 6 items (CFI=0.946; RMSEA=0.071; SRMR=0.035) and the health services management scale with 8 items (CFI=0.972; RMSEA=0.081; SRMR=0.028) showed good measurement properties for the one-dimensional model. The scale of working conditions presented adequate measurement properties with a two-dimensional model (CFI=0.914; RMSEA=0.080; SRMR=0.055): individual conditions (8 items) and infrastructural conditions (3 items). The invariance analysis presented that comparisons can be made between sex, age, civil status, medical specialty, working in other institutions, work-related illness, chronic disease, and time working in health care center. All scales had adequate internal consistency (ω and α between 0.70 and 0.90).Conclusions: The set of scales solid factorial structure and measurement invariance, which makes it possible for group comparison. They also achieved stability in their scores as they showed adequate internal consistency coefficients. Based on our findings, these instruments are suitable for measuring job satisfaction among outpatient physicians throughout Peru, as our data is representative at the country level.
Abnormal Electrophysiological Phenotypes and Sleep Deficits in a Mouse Model of Angelman Syndrome
Authors: Copping Nycole A , Silverman Jill L 
Published: September 2020

Abstract
Background: Angelman Syndrome (AS) is a rare genetic disorder characterized by impaired communication, motor and balance deficits, intellectual disabilities, recurring seizures and abnormal sleep patterns. The genetic cause of AS is neuronal specific loss of expression of UBE3A (ubiquitin-protein ligase E6-AP), an imprinted gene. Seizure and sleep disorders are highly prevalent (>80%) in the AS population. The present experiments were designed to identify translational, neurophysiological outcome measures in a model of AS.Methods: We used the exon-2 deletion mouse (Ube3a-del) on a C57BL/6J background to assess seizure, sleep and electrophysiological phenotypes. Seizure susceptibility has been reported in Ube3a-del mice with a variety of seizure induction methods. Here, we provoked seizures by a single high-dose injection of 80 mg/kg pentylenetetrazole. Novel experiments included the utilization of wireless telemetry devices to acquire global electroencephalogram (EEG) and neurophysiological data on electrographic seizures, power spectra, light/dark cycles, sleep stages and sleep spindles in Ube3a-del and WT mice.Results: Ube3a-del mice exhibited reduced seizure threshold compared to WT. EEG illustrated that Ube3a-del mice had increased epileptiform spiking activity and delta power, which corroborates findings from other laboratories and recapitulates clinical reports in AS. This is the first report to use a cortical surface-based recording by a wireless telemetry device over tethered/fixed head-mount depth recordings. Less time in both paradoxical and slow-wave sleep, longer latencies to paradoxical sleep stages, and total less sleep time in Ube3a-del mice were observed compared to WT. For the first time, we detected fewer sleep spindles in the AS mouse model.Limitations: This study was limited to the exon 2 deletion mouse model, future work will investigate the rat model of AS, containing a complete Ube3a deletion and pair EEG with behavior.Conclusions: Our data enhance rigor and translatability as our study provides important corroboration of previous reports on epileptiform and elevated delta power. For the first-time we report neurophysiological phenotypes collected via translational methodology. Furthermore, this is the first report of reduced sleep spindles, a critical marker of memory consolidation during sleep, in an AS model. Our results are useful outcomes for therapeutic testing.
Just Like Fireworks in My Brain – An Interview Study on Experiences of Emotions in Patients with Eating Disorders
Authors: Petersson Suzanne , Gullbing Lydia , Perseius Kent-Inge 
Published: September 2020

Abstract
Background: Patients with eating disorders have reported poorer emotional awareness, more emotional suppression, less use of adaptive emotional regulation strategies, and more use of maladaptive emotional regulation strategies compared to people in healthy control groups. Aim: To explore experiences of emotions by a sample of patients with eating disorders. Method: Nine patients with eating disorder diagnoses at an eating disorder outpatient clinic in Sweden were interviewed for their thoughts on emotions. The interviews were analyzed with Thematic Analysis. Result: Four themes were constructed: "Not knowing what one feels", "Switch off, run away, or hide behind a mask", "Emotions in a lifelong perspective", and "Using eating behaviors to regulate emotions". The patients described uncertainty regarding whether they experienced emotions correctly. They described how they tried to avoid difficult emotions through suppressive strategies and eating disorder behavior. All described strategies were inefficient and all emotions were experienced as problematic, even joy. Since joy was used as a mask, the real experience of happiness was lost and mourned.Conclusion: All kinds of emotions were considered problematic to experience, but shame, fear, and sadness were considered worst. It is difficult to know if the emotional difficulties preceded an eating disorder, but at least those problems seem to have increased by the eating disorder.