bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utility of major basic protein, eotaxin-3, and mast cell tryptase staining for prediction of response to topical steroid treatment in eosinophilic esophagitis: analysis of a randomized, double-blind, double dummy clinical trial
Authors: Dellon Evan S , Woosley John T , McGee Sarah J , Moist Susan E , Shaheen Nicholas J 
Published: March 2020

Summary
Inflammatory factors in eosinophilic esophagitis (EoE), including major basic protein (MBP), eotaxin-3 (EOT3) and mast cell tryptase (TRP), may predict treatment response to topical corticosteroids (tCS). We aimed to determine whether baseline levels of these markers predict response to tCS for EoE. To do this, we analyzed data from a randomized trial comparing two topical steroids for treatment of newly diagnosed EoE (NCT02019758). A pretreatment esophageal biopsy was stained for MBP, EOT3, and TRP to quantify tissue biomarker levels (cells/mm2). Levels were compared between histologic responders (&lt;15 eos/hpf) and nonresponders (the primary outcome), and endoscopic responders (EREFS&lt;2) and nonresponders. Complete histologic response (&lt;1 eos/hpf) was also assessed, and area under the receiver operator characteristic curve (AUC) was calculated. We also evaluated whether baseline staining predicted symptom relapse in the trial’s off-treatment observation phase. Baseline samples were evaluable in 110/111 subjects who completed the randomized trial. MBP levels were higher in nonresponders (n = 36) than responders (704 vs. 373 cells/mm2; P = 0.007), but EOT3 and TRP levels were not statistically different. The combination of all three stains had an AUC of 0.66 to predict response. For complete histologic response, baseline TRP levels were higher in nonresponders (n = 69) than responders (370 vs. 268 mast cells/mm2; P = 0.01), with an AUC of 0.65. The AUC for endoscopic response was 0.68. Baseline staining did not predict symptom recurrence after remission. Pretreatment MBP, EOT3, and TRP levels were not strongly or consistently associated with histologic or endoscopic response to topical steroids. While elevated TRP levels may be associated with nonresponse compared with complete response, the magnitude and predictive utilities were modest. Novel methods for predicting steroid response are still required.
Treatment protocol for secondary esophageal reconstruction using ‘supercharged’ colon interposition flaps
Authors: Esmonde N , Rodan W , Haisley K R , Joslyn N , Carboy J , Hunter J G , Schipper P H , Tieu B H , Hansen J , Dolan J P 
Published: March 2020

Abstract
Locoregional esophageal cancer is currently treated with induction chemoradiotherapy, followed by esophagectomy with reconstruction, using a gastric conduit. In cases of conduit failure, patients are temporized with a cervical esophagostomy and enteral nutrition until gastrointestinal continuity can be established. At our institution, we favor reconstruction, using a colon interposition with a ‘supercharged’ accessory vascular pedicle. Consequently, we sought to examine our technique and outcomes for esophageal reconstruction, using this approach. We performed a retrospective review of all patients who underwent esophagectomy at our center between 2008 and 2018. We identified those patients who had a failed gastric conduit and underwent secondary reconstruction. Patient demographics, perioperative details, and clinical outcomes were analyzed after our clinical care pathway was used to manage and prepare patients for a second major reconstructive surgery. Three hundred and eighty eight patients underwent esophagectomy and reconstruction with a gastric conduit. Seven patients (1.8%) suffered gastric conduit loss and underwent a secondary reconstruction using a colon interposition with a ‘supercharged’ vascular pedicle. Mean age was 70.1 (±7.3) years, and six patients were male. The transverse colon was used in four cases (57.1%), left colon in two cases (28.6%), and right colon in one case (14.3%). There were no deaths or loss of the colon interposition at follow-up. Three patients (42.9%) developed an anastomotic leak, which resolved with conservative management. All patients had resumption of oral intake within 30 days. Utilizing a ‘supercharging’ technique for colon interposition may improve the perfusion to the organ and may decrease morbidity. Secondary reconstruction should occur when the patient’s oncologic, physiologic, and psychosocial condition is optimized. Our outcomes and preoperative strategies may provide guidance for those centers treating this complicated patient population.
Insights into the community structure and lifestyle of the fungal root endophytes of tomato by combining amplicon sequencing and isolation approaches with phytohormone profiling
Authors: Manzotti Andrea , Bergna Alessandro , Burow Meike , Jørgensen Hans J L , Cernava Tomislav , Berg Gabriele , Collinge David B , Jensen Birgit 
Published: March 2020

ABSTRACT
Little is known about the influence of host genotype and phytohormones on the composition of fungal endophytic communities. We investigated the influence of host genotype and phytohormones on the structure of the fungal endophytic communities of tomato roots by amplicon sequencing of the ITS1 region and combined this approach with isolation and functional characterization of the isolates. A significant effect of the host genotype on the dominant fungal species was found by comparing the cultivars Castlemart and UC82B and, surprisingly, root pathogens were among the most abundant taxa. In contrast, smaller changes in the relative abundance of the dominant species were found in mutants impaired in jasmonic acid biosynthesis (def1) and ethylene biosynthesis (8338) compared to the respective wild types. However, def1 showed significantly higher species richness compared to the wild type. Analysis of the phytohormone profiles of these genotypes indicates that changes in the phytohormone balance may contribute to this difference in species richness. Assessing the lifestyle of isolated fungi on tomato seedlings revealed the presence of both beneficial endophytes and latent pathogens in roots of asymptomatic plants, suggesting that the interactions between members of the microbiome maintain the equilibrium in the community preventing pathogens from causing disease.
Introducing a Face Sort Paradigm to Evaluate Age Differences in Emotion Perception
Authors: Hoemann Katie , Vicaria Ishabel M , Gendron Maria , Stanley Jennifer Tehan 
Published: March 2020

Abstract

Objectives
Previous research has uncovered age-related differences in emotion perception. To date, studies have relied heavily on forced-choice methods that stipulate possible responses. These constrained methods limit discovery of variation in emotion perception, which may be due to subtle differences in underlying concepts for emotion.

Method
We employed a face sort paradigm in which young (N = 42) and older adult (N = 43) participants were given 120 photographs portraying six target emotions (anger, disgust, fear, happiness, sadness, and neutral) and were instructed to create and label piles, such that individuals in each pile were feeling the same way.

Results
There were no age differences in number of piles created, nor in how well labels mapped onto the target emotion categories. However, older adults demonstrated lower consistency in sorting, such that fewer photographs in a given pile belonged to the same target emotion category. At the same time, older adults labeled piles using emotion words that were acquired later in development, and thus are considered more semantically complex.

Discussion
These findings partially support the hypothesis that older adults’ concepts for emotions and emotional expressions are more complex than those of young adults, demonstrate the utility of incorporating less constrained experimental methods into the investigation of age-related differences in emotion perception, and are consistent with existing evidence of increased cognitive and emotional complexity in adulthood.
Metabolomics hallmarks OPA1 variants correlating with their in vitro phenotype and predicting clinical severity
Authors: Chao de la Barca Juan Manuel , Fogazza Mario , Rugolo Michela , Chupin Stéphanie , Del Dotto Valentina , Ghelli Anna Maria , Carelli Valerio , Simard Gilles , Procaccio Vincent , Bonneau Dominique , Lenaers Guy , Reynier Pascal , Zanna Claudia 
Published: March 2020

Abstract
Interpretation of variants of uncertain significance is an actual major challenge. We addressed this question on a set of OPA1 missense variants responsible for variable severity of neurological impairments. We used targeted metabolomics to explore the different signatures of OPA1 variants expressed in Opa1 deleted mouse embryonic fibroblasts (Opa1−/− MEFs), grown under selective conditions. Multivariate analyses of data discriminated Opa1+/+ from Opa1−/− MEFs metabolic signatures and classified OPA1 variants according to their in vitro severity. Indeed, the mild p.I382M hypomorphic variant was segregating close to the wild-type allele, while the most severe p.R445H variant was close to Opa1−/− MEFs, and the p.D603H and p.G439V alleles, responsible for isolated and syndromic presentations, respectively, were intermediary between the p.I382M and the p.R445H variants. The most discriminant metabolic features were hydroxyproline, the spermine/spermidine ratio, amino acid pool and several phospholipids, emphasizing proteostasis, endoplasmic reticulum (ER) stress and phospholipid remodeling as the main mechanisms ranking OPA1 allele impacts on metabolism. These results demonstrate the high resolving power of metabolomics in hierarchizing OPA1 missense mutations by their in vitro severity, fitting clinical expressivity. This suggests that our methodological approach can be used to discriminate the pathological significance of variants in genes responsible for other rare metabolic diseases and may be instrumental to select possible compounds eligible for supplementation treatment.
Effects of climate and spawning stock structure on the spatial distribution of Northeast Arctic cod larvae
Authors: Endo Clarissa Akemi Kajiya , Vikebø Frode B , Yaragina Natalia A , Hjøllo Solfrid Sætre , Stige Leif Christian 
Published: March 2020

AbstractThe spatial distribution of fish early life stages can impact recruitment at later stages and affect population size and resilience. Northeast Arctic (NEA) cod spawning occurs along the Norwegian coast. Eggs, larvae, and pelagic juveniles drift near-surface towards the Barents Sea nursery area. In this study, a 35-year long time series of NEA cod larvae data was analysed in combination with factors that potentially may affect the distribution of eggs and larvae. These factors included biological aspects of the spawning stock, and environmental variables, such as water temperature, wind, ocean current, and prey abundance. Our aim was to shed light on how these factors influence larval abundance and distribution and how larval abundance and distribution influenced recruitment at age 3. We found that biomass and mean weight of the spawners were positively associated with larval abundance and that a high liver condition index of the spawners was associated with a north-easterly distribution of the larvae. The environmental variables showed generally weak or no correlations with abundance or distribution of larvae. Lastly, we found significant association between larval abundance and year-class abundance at age 3, while the spatial distribution metrics of the larvae, i.e. distribution extent, mean longitude, and mean latitude, showed no significant association with future year-class abundance.
Minimal Bubbling for Willmore Surfaces
Authors: Marque Nicolas 
Published: March 2020

AbstractIn this paper, we build an explicit example of a minimal bubble on a Willmore surface, showing there cannot be compactness for Willmore immersions of Willmore energy above $16 \pi $. Additionally, we prove an inequality on the 2nd residue for limits of sequences of Willmore immersions with simple minimal bubbles. Doing so, we exclude some gluing configurations and prove compactness for immersed Willmore tori of energy below $12 \pi $.
National monitoring and evaluation of eHealth: a scoping review
Authors: Villumsen Sidsel , Adler-Milstein Julia , Nøhr Christian 
Published: March 2020

AbstractObjectiveThere has been substantial growth in eHealth over the past decade, driven by expectations of improved healthcare system performance. Despite substantial eHealth investment, little is known about the monitoring and evaluation strategies for gauging progress in eHealth availability and use. This scoping review aims to map the existing literature and depict the predominant approaches and methodological recommendations to national and regional monitoring and evaluation of eHealth availability and use, to advance national strategies for monitoring and evaluating eHealth.MethodsPeer-reviewed and grey literature on monitoring and evaluation of eHealth availability and use published between January 1, 2009, and March 11, 2019, were eligible for inclusion. A total of 2354 publications were identified and 36 publications were included after full-text review. Data on publication type (eg, empirical research), country, level (national or regional), publication year, method (eg, survey), and domain (eg, provider-centric electronic record) were charted.ResultsThe majority of publications monitored availability alone or applied a combination of availability and use measures. Surveys were the most common data collection method (used in 86% of the publications). Organization for Economic Co-operation and Development (OECD), European Commission, Canada Health Infoway, and World Health Organization (WHO) have developed comprehensive eHealth monitoring and evaluation methodology recommendations.DiscussionEstablishing continuous national eHealth monitoring and evaluation, based on international approaches and recommendations, could improve the ability for cross-country benchmarking and learning. This scoping review provides an overview of the predominant approaches to and recommendations for national and regional monitoring and evaluation of eHealth. It thereby provides a starting point for developing national eHealth monitoring strategies.
A Biodegradable Formulation of MCH (3-Methylcyclohex-2-en-1-one) for Protecting Pseudotsuga menziesii from Dendroctonus pseudotsugae (Coleoptera: Curculionidae) Colonization
Authors: Foote Gabriel G , Fettig Christopher J , Ross Darrell W , Runyon Justin B , Coleman Tom W , Gaylord Monica L , Graves Andrew D , McMillin Joel D , Mortenson Leif A , Mafra-Neto Agenor 
Published: March 2020

Abstract
Douglas-fir, Pseudotsuga menziesii (Mirb.) Franco, trees and stands can be protected from Douglas-fir beetle, Dendroctonus pseudotsugae Hopkins (DFB)-caused mortality by application of synthetic formulations of the beetle’s antiaggregation pheromone, 3-methylcyclohex-2-en-1-one (MCH). A biodegradable formulation of MCH, SPLAT MCH, was developed and evaluated for protecting individual Douglas-fir trees and small stands from colonization and mortality by DFB. In an individual-tree experiment in Idaho, both MCH bubble capsules and SPLAT MCH significantly reduced the proportion of treated trees colonized and killed by DFB compared to untreated controls. SPLAT MCH was as effective as MCH bubble capsules for protecting individual trees. Both MCH bubble capsules and SPLAT MCH significantly reduced the proportion of trees colonized and killed by DFB within 0.04-ha circular plots surrounding each treated tree compared to untreated controls. In 0.41 ha stands in New Mexico, both MCH bubble capsules and SPLAT MCH significantly reduced the proportion of trees colonized and killed by DFB compared to untreated controls, again with no differences observed between MCH treatments. In a similar stand level trial in Idaho, neither MCH treatment significantly reduced the proportion of trees colonized by DFB, and only MCH bubble capsules significantly reduced levels of tree mortality compared to untreated controls, but no significant difference was observed between SPLAT MCH and MCH bubble capsules. Overall, the results indicate that SPLAT MCH is as effective as MCH bubble capsules for protecting individual trees and small stands of Douglas-fir from DFB-caused mortality.
Negative Life History Impacts for Habrobracon hebetor (Hymneoptera: Braconidae) that Develop in Bollworm Larvae Inoculated with Helicoverpa armigera Nucleopolyhedrovirus
Authors: Allahyari Rahim , Aramideh Shahram , Michaud J P , Safaralizadeh Mohammad Hassan , Rezapanah Mohammad Reza 
Published: March 2020

AbstractCotton bollworm, Helicoverpa armigera Hubner, is a cosmopolitan polyphagous pest of many crops. Habrobracon hebetor Say and Helicoverpa armigera nucleopolyhedrovirus (HearNPV) are two important biocontrol agents used to manage this pest, sometimes in combination. We evaluated the sublethal effects of HearNPV on H. hebetor life table parameters under laboratory conditions when its host (second instar H. armigera) was treated with HearNPV, and tested H. hebetor females for their ability to discriminate against inoculated hosts. Emergence of adults reared as solitary larvae was reduced by half on LC30-inoculated larvae compared to controls, but not on LC5 or LC15-inoculated hosts. Low concentrations (LC5, LC15, and LC30) of HearNPV had no effects on overall parasitoid developmental time, but longevity and lifetime fecundity was reduced for females emerging from hosts receiving the LC30 treatment. Net reproductive rate (R0), intrinsic rate of increase (rm), and finite rate of increase (λ) were all decreased in a concentration-dependent manner in the LC15 and LC30 treatments, as were female life expectancy, age-specific survivorship (lx), and age-specific fecundity (mx), whereas population doubling time (DT) increased. Parasitoids did not discriminate against LC15-inoculated larvae in choice or no-choice tests, but parasitized more LC50-inoculated hosts than controls in the choice test, with no significant differences in total numbers of eggs laid in either case. Although parasitoids suffered some loss of fitness in HearNPV-inoculated hosts under these laboratory conditions, these agents still appear compatible for joint application against H. armigera under field conditions, provided parasitoid releases are made 2 d after NPV application.
Integrative Transcriptome and Proteome Analyses Provide New Insights Into the Interaction Between Live Borrelia burgdorferi and Frontal Cortex Explants of the Rhesus Brain
Authors: Ding Zhe , Sun Luyun , Bi Yunfeng , Zhang Yu , Yue Peng , Xu Xin , Cao Wenjing , Luo Lisha , Chen Taigui , Li Lianbao , Ji Zhenhua , Jian Miaomiao , Lu Lihong , Abi Manzama-Esso , Liu Aihua , Bao Fukai 
Published: March 2020

Abstract
Borrelia burgdorferi (Bb), which is neurotropic, can attack the central nervous system (CNS), leading to the development of various neurologic symptoms. The pathogenesis of Lyme neuroborreliosis (LNB) remains poorly understood. Presently, there is a lack of knowledge of the changes in mRNA and proteins in the CNS following early disseminated Lyme disease. Explants from the frontal cortex of 3 rhesus brains were incubated with medium alone or with medium containing live Bb for 6, 12, or 24 hours. Then, we analyzed identified mRNA and proteins in the frontal cortex tissues, allowing for an in-depth view of the transcriptome and proteome for a macroscopic and unbiased understanding of early disseminated Lyme disease in the brain. Through bioinformatics analysis, a complex network of enriched pathways that were mobilized during the progression of Lyme spirochete infection was described. Furthermore, based on the analysis of omics data, translational regulation, glycosaminoglycan/proteoglycan-binding activity in colonization and dissemination to tissues, disease-associated genes, and synaptic function were enriched, which potentially play a role in pathogenesis during the interaction between frontal cortex tissues and spirochetes. These integrated omics results provide unbiased and comprehensive information for the further understanding of the molecular mechanisms of LNB.
Giant Appendicolith: A Case Report and Review of the Literature
Authors: Pahissa Robert A , Lin-Hurtubise Kevin M 
Published: March 2020

Abstract
Acute appendicitis is one of the most prevalent causes of an acute abdomen. Although the cause of appendicitis is not completely understood, the theory of luminal obstruction is a popular belief, with appendicoliths being a common etiology. While appendicoliths are quite common, giant appendicoliths &gt;2 cm are rare. Although previous reports cite only two or three other occurrences of giant appendicoliths, we found at least 11 reported cases in the literature. We present a young male diagnosed preoperatively on computed tomography to have a large appendiceal mass of 2.2 cm. This case is presented for the rarity of giant appendicoliths along with a review of the literature.
Radiation forces constrain the FRB mechanism
Authors: Kumar Pawan , Lu Wenbin 
Published: March 2020

ABSTRACT
We provide constraints on fast radio burst (FRB) models by careful considerations of radiation forces associated with these powerful transients. We find that the induced Compton scatterings of the coherent radiation by electrons/positrons accelerate particles to very large Lorentz factors (LFs) in and around the source of this radiation. This severely restricts those models for FRBs that invoke relativistic shocks and maser-type instabilities at distances less than about 1013 cm of the neutron star. Radiation travelling upstream, in these models, forces particles to move away from the shock with an LF larger than the LF of the shock front. This suspends the photon generation process after it has been operating for less than ∼0.1 ms (observer frame duration). We show that masers operating in shocks at distances larger than 1013 cm cannot simultaneously account for the burst duration of 1 ms or more and the observed ∼GHz frequencies of FRBs without requiring an excessive energy budget (&gt;1046 erg); the energy is not calculated by imposing any efficiency consideration, or other details, for the maser mechanism, but is entirely the result of ensuring that particle acceleration by induced Compton forces upstream of the shock front does not choke off the maser process. For the source to operate more or less continuously for a few ms, it should be embedded in a strong magnetic field – cyclotron frequency ≫ wave frequency – so that radiation forces do not disperse the plasma and shut off the engine.