bluuebunny/crossref_metadata_2025_split huggingface dataset:
208 Effects of supplemental guanidinoacetic acid, creatine, and choline on protein deposition and methyl group metabolites in growing steers
Authors: Grant Madeline S , Miesner Matt D , Titgemeyer Evan C 
Published: November 2020

Abstract
Methyl group donors (e.g., methionine, choline) can improve performance in transition dairy cows. Our objective was to determine how modulation of methyl group status would affect protein deposition and plasma and urine metabolites associated with methyl group metabolism. Six ruminally cannulated Holstein steers (200 kg) were used in a 6×6 Latin square design with 10-d periods. Factorial treatments, which were continuously infused abomasally, included 3 methyl group modulators (MGM: control; 15 g/d guanidinoacetic acid [GAA]; or 16.8 g/d creatine) and 2 levels of choline (0 or 5 g/d choline ion). Providing GAA or creatine increases body creatine supply; however, GAA consumes methyl groups to synthesize creatine, whereas supplemental creatine directly spares methyl groups that would be used for its synthesis. Steers received 4 kg/d of a corn-based diet. Total collection of urine and feces occurred from d 7 to 9 to measure N retention. Jugular blood was collected on d 10. No interactions between MGM and choline were observed. GAA increased N retention (P = 0.04), whereas creatine did not. Urinary creatine excretion was increased by GAA and creatine supplementation (P &lt; 0.0001). Urinary GAA output was greater for GAA than creatine and control (P &lt; 0.0001). Plasma creatine concentration was increased by GAA and creatine supplementation (P ≤0.01), with GAA leading to a larger increase. Plasma GAA concentration increased with GAA provision (P &lt; 0.0001) but tended to be decreased by creatine (P = 0.09). Plasma urea-N and urinary urea-N output were not affected by MGM (P ≥ 0.25). Choline did not affect N retention (P = 0.69) or urinary GAA or creatine excretions (P ≥ 0.14). Choline increased plasma creatine (P = 0.04), but not plasma GAA. Choline did not affect plasma urea-N or urinary urea-N output (P ≥ 0.68). These data demonstrate that GAA has potential to improve protein deposition in growing cattle. Additionally, choline provision may allow for greater creatine synthesis in the body.
223 Evaluation of dietary characteristics and empty body chemical components affecting the relationship between heat production and metabolizable energy intake in growing beef cattle
Authors: Lancaster Phillip A 
Published: November 2020

Abstract
Metabolizable energy required for maintenance varies with diet and empty body chemical composition. The objective was to quantify the relationships of dietary characteristics and empty body chemical composition with heat production. A literature search was performed to compile data (31 studies, 214 treatment means) on metabolizable energy intake (MEI) and composition of empty body gain in growing steers and heifers. Data analysis were performed using R statistical package for mixed models with study as random variable. Nonlinear regression of energy gain (EG) on MEI indicated the relationship was not curvilinear in this data set, likely due to lack of negative values of EG. Further analyses were conducted using a linear model. Dietary characteristics of roughage level (0–100% of diet DM) and CP (10–25% diet DM), metabolizable energy concentration (1.3–3.3 Mcal/kg DM), and roughage type were evaluated in the model. Roughage sources were categorized into no roughage, silage, hay, pellets, silage + pellets, and hay + pellets. Of the empty body chemical components, proportion of fat in the empty body (EBFp) and in the gain (EBFgp) had a significant (P &lt; 0.001) interaction with MEI on HP. Of the dietary characteristics, roughage level and type had a significant (P &lt; 0.001) interaction with MEI on HP; however, when both were included in the model, roughage type was not significant (P &gt; 0.10). The final model was 47.01 ± 12.54 + 0.630 ± 0.05*MEI – 132.3 ± 64.7*EBFp + 0.0007 ± 0.0001*MEI*Roughage level + 0.753 ± 0.24*MEI*EBFp – 0.268 ± 0.032*MEI*EBFgp with an R2 of 0.919 and an AIC of 1614 compared with 0.867 and 1695 for the simple linear regression model of HP on MEI. In conclusion, greater empty body fat decreased the intercept, and greater empty body fat proportion and levels of roughage in the diet increased the slope between HP and MEI, whereas greater percentage of fat in the empty body gain decreased the slope between HP and MEI.
187 Effects of energy supplementation during early gestation in beef heifers on body weight, concentrations of IGF-1, and calf characteristics
Authors: Baumgaertner Friederike , Underdahl Sarah R , McCarthy Kacie L , Menezes Ana Clara B , da Silva Diniz Wellison Jarles , Ward Alison K , Sedivec Kevin K , Dorsam Sheri T , Kirsch James D , Caton Joel , Dahlen Carl R 
Published: November 2020

Abstract
Objectives were to evaluate effects of early gestation energy supplementation on heifer body weight (BW), IGF-1 concentrations, and calf characteristics. One-hundred Angus-based heifers (initial BW = 371.1 ± 3.9 kg) were estrous synchronized, bred to female-sexed semen, then randomly assigned to either a basal diet to achieve 0.20 kg/d (CON, n = 50) or a basal diet plus supplemental energy to achieve 0.75 kg/d gain (SUPP, n = 50) for 84 d. The CON diet consisted of 37% corn silage, 53% prairie hay, and 10% distillers dried grain with solubles (DDGS). The SUPP diet was 29% corn silage, 41% prairie hay, 5% DDGS, and 25% corn/PurinaAccuration Range Supplement 33 blend. Heifers were individually fed via Insentec feeders (Insentec Marknesse, The Netherlands), with bi-weekly feed delivery adjustments to achieve targeted gains. After d 84, heifers were managed as a single group on pasture, then in a dry lot until parturition. A total of 46 heifers gestated female pregnancies to term (n = 23 CON and n = 23 SUPP). Body weights and blood samples were collected on d 0, 42, 84, 162, 234, 262, and at parturition. By design, SUPP heifers were 55.4 kg heavier (P &lt; 0.0001) than CON at d 84. Weight divergence remained throughout gestation, with SUPP heifers being 41.5 kg heavier at parturition (P &lt; 0.0001). For SUPP, IGF-1 concentrations were greater throughout gestation (P = 0.003). Fetal size, as measured by ultrasound, was not affected by treatment at d 42 (P = 0.50), 63 (P = 0.35), or 84 (P = 0.20); however, calves from SUPP dams were 2.1 kg heavier (P = 0.03) and had larger chest circumference (P = 0.05) at birth compared with CON. Early gestation nutrition impacted heifer BW and IGF-1 concentrations throughout gestation, and clearly impacted in utero fetal growth.
393 Awardee Talk: Effect of trace mineral source on rumen fermentation and trace mineral distribution in the rumen
Authors: Engle Terry E 
Published: November 2020

Abstract
The role that Cu and Zn play in rumen microbial fermentation is not well understood. Microorganisms use small proportions of dietary Cu and Zn for catalytic, structural, and stabilizing functions. In order for microorganisms to acquire Cu and Zn, the elements must be soluble in the rumen environment. Data would indicate that practical diets fed to ruminants without Cu and Zn supplementation are able to meet the microbial Cu and Zn requirements. Therefore, rumen solubility of supplemental Cu and Zn can impact rumen microbial fermentation characteristics. Numerous factors can impact rumen solubility of minerals, such as the pH of the rumen, the concentration of dietary antagonists (Mo, Fe, S, fiber, etc.), and mineral source. Earlier research has indicated that high soluble concentrations of Cu and Zn in the rumen environment can become toxic to certain rumen microorganisms and decrease fiber digestion. Recent research from our laboratory has indicated that hydroxy trace mineral (HTM) forms of Cu and Zn are relatively insoluble in the rumen and that a greater proportion of HTM remained loosely bound to the insoluble fraction within the rumen when compared to sulfate trace mineral (STM) sources of Cu and Zn. We have also reported that supplemental sources of Cu and Zn that are highly soluble in the rumen environment (STM) can decrease fiber digestion, reduce total VFA concentrations, and can become more tightly bound to rumen solid digesta than HTM sources of Cu and Zn. The stronger binding of STM relative to HTM to the solid rumen digesta fraction may reduce absorption in the small intestine. Future research investigating the flow and passage rate of different trace mineral sources through the abomasum into the duodenum and duodenal absorption efficiency of Cu and Zn from different TM sources is needed.
252 Better Preparing Animal Science Students for Education Associated Careers
Authors: Barnes Taylor , Burnett Kirstin M , Ramsey W Shawn , Dunlap Kathrin 
Published: November 2020

Abstract
An Animal Science degree prepares students for a broad range of career opportunities yet the two most discussed in the literature and assumed by society are in animal science industry or veterinary medicine. However, studies indicate that -60% of our students enter into education as a career path, yet animal science departments typically do not emphasize educational careers or provide opportunities for students to develop skills in methods relevant for animal science related teaching. To combat this lack of representation for educational career opportunities, our research university’s animal science department has acted in two ways:-Developed a laboratory teaching methods course that allows undergraduate students to serve as teaching assistants for an animal handling lab with support and supervision from the course and graduate student lab instructors-Performed a content analysis to identify the type, frequency, and way educational career paths are discussed and/or assessed in introductory animal science courses. These are the first steps in ensuring that students are as prepared for pursuit of a possible educational career at a level equal to that for industry or veterinary careers. Sustainability of animal science includes retaining students in educational careers and actively improving our educational practices within the discipline. These changes better prepare our undergraduate students to have more realistic job expectations and competence in teaching when they pursue graduate school or animal science related educational career options. The university animal science program also benefits, as the undergraduate teaching assistants improve the instructor-to-student ratio thus positively impacting safety and logistics of hands-on large animal labs while increasing one-on-one instruction time with students. These practices increase student engagement and learning. Additionally, the field of animal science will prosper from increased competence in pedagogical techniques providing a richer, more complete educational experience for our students both in and out of university settings.
412 Germ and somatic cell interactions during oocyte development and maturation
Authors: Perecin Felipe 
Published: November 2020

Abstract
Ovarian follicle development and oocyte competence acquisition is dependent upon continuous interactions between the somatic cells and the oocyte. Interactions between these cell types include bidirectional paracrine signaling and the exchange of small molecules, such and amino acids and cyclic nucleotides, through gap junctions located at the end of transzonal projections (TZPs). In the last decade, additional mechanisms of cell-to-cell interactions within the ovarian follicle were described. These mechanisms include the movement of small extracellular vesicles (EVs) within the follicular fluid and the delivery of its cargo to target cells; and the exchange of large molecules transiting from the cumulus cells to the oocytes via transzonal projections. Here, I will describe the investigations about these novel communication systems in the bovine ovarian follicle. The topics will include the content of EVs transiting in the bovine follicular fluid and its role regulating signaling pathways associated with oocyte competence, and the movement of large molecules from cumulus cell to the oocyte such as messenger RNAs and fatty acids. Finally, dysregulations of such communications mechanisms under in vitro culture conditions will also be reviewed. Emphasis will be given on the lipid metabolism in the cumulus-oocyte complex and lipid accumulation mediated by transzonal projections and fatty acid binding proteins in oocytes undergoing in vitro maturation.
264 Harvest season, carcass weight, and fat measurement effects on lamb carcass characteristics and economic comparison of moderate and heavy weight lamb carcasses in the Western lamb processing industry
Authors: Whaley Jaelyn , Means Warrie , Ritten John , Murphy Tom , Gifford Cody , Cunningham Hannah , Woodruff Kelly , McKibben Heather , Page Chad M , Stewart Whit C 
Published: November 2020

Abstract
Carcass characteristics and economic impact estimates of over-finished lambs on the processing sector were evaluated in two commercial Intermountain West abattoirs. Lamb carcasses were surveyed throughout the year using digital images and imaging software (n = 9,532). Estimations of abattoir costs and returns included loading labor, downtime cost, price of fat, live and carcass trucking costs from the two largest lamb processors in the Intermountain West. Profitability comparisons were made using Monte Carlo simulation models replicating live and carcass prices for distributions based on historical pricing data to assess overall profitability of a carcass in an ideal weight range (29.5 - 39.0 kg) and a carcass that exceeds ideal weight (&gt; 39.0 kg). Overall means show that the average lamb carcass exceeded packer preferred hot carcass weight (40.76 ± 9.29 kg) and industry acceptable 12th rib fat thickness (8.17 ± 3.79 mm). There were seasonal differences in hot carcass weight and fat measurements with carcasses being lighter weight (P = 0.05) and trimmer (P = 0.05) in the summer months. Monte Carlo simulation found that the additional yield from heavier carcasses offset costs of harvesting them. However, factors such as machine wear and increased labor turnover rates should be considered, although difficult to quantify. Collectively, the current study shows that U.S. lamb carcasses are too heavy and excessively fat but have minor effect on processor profitability.
268 Administering an appeasing substance to optimize welfare and performance of receiving cattle
Authors: Colombo Eduardo , Cooke Reinaldo F , Brandão Alice , Wiegand Jacob , Schubach Kelsey , Sowers CiCi A , Duff Glenn , Gouvea Vinicius N , Cappellozza Bruno I 
Published: November 2020

Abstract
This experiment evaluated the impacts of bovine appeasing substance (BAS) administration on performance, health, and physiological responses of feedlot cattle during a 45-d receiving period. A total of 342 recently-weaned Angus-influenced steers, originating from 16 cow-calf operations, were obtained from an auction yard on d -1 and road-transported (12 h) to the feedlot. Upon arrival on d 0, body weight (BW) was recorded and steers were ranked by BW and source and assigned to receive BAS (Nutricorp, Araras, SP, Brazil; n = 171) or placebo (diethylene glycol monoethyl ether; CON; n = 171). Treatments (5 mL) were topically applied to the nuchal skin area of each animal. Within treatment, calves were allocated to 1 of 24 drylot pens (12 pens/treatment) and received a free choice total-mixed ration from d 1 to 45. Calves were assessed for bovine respiratory disease (BRD) signs and feed intake was recorded from each pen daily. Steer BW was again recorded on d 1, 7, 17, 31, and 45, whereas blood samples were collected from 5 steers/pen concurrently with each BW assessment. Average daily gain was greater (P = 0.05) in BAS vs. CON calves, although final BW did not differ (P = 0.36) between treatments. No treatment effects were detected for feed intake (P = 0.95), resulting in greater (P = 0.05) feed efficiency in BAS vs. CON steers. No treatment effects were detected (P ≥ 0.37) for plasma concentrations of haptoglobin, whereas plasma cortisol concentrations were greater (P = 0.05) in CON vs. BAS steers on d 7 (treatment × day; P = 0.07). Incidence of BRD was greater (P ≤ 0.05) in BAS vs. CON on d 6 to 10 and d 18 to 21 (treatment × day; P &lt; 0.01), although overall BRD incidence did not differ (P = 0.24) between treatments. The number of antimicrobial treatments required per steer diagnosed with BRD symptoms to recover from sickness was greater (P = 0.04) in CON vs. BAS calves. No treatment differences were detected (P ≥ 0.41) for mortality incidence, or proportion of steers removed from the experiment due to extreme sickness. Results from this experiment indicate BAS administration upon feedlot entry improved average daily gain by enhancing feed efficiency. Administration of BAS facilitated earlier detection of BRD and reduced the need for antimicrobial treatments. Collectively, these results suggest BAS administration as a promising strategy to benefit performance and immunocompetence of feedlot receiving cattle.
406 Maternal nutrition during early gestation: Impacts on developmental outcomes
Authors: Caton Joel , Crouse Matthew S , Reynolds Lawrence P , Dahlen Carl R , Neville Bryan W , Borowicz Pawel P , Neville Tammi L , McLean Kyle J , Ward Alison K 
Published: November 2020

Abstract
Changes in maternal nutrition that represent inadequacies or excesses in nutrient supply have been implicated in altered fetal development as well as offspring outcomes and have been termed fetal or developmental programming. Research in the area of developmental programming of livestock has greatly increased during the last two decades. For ruminants, most research efforts in developmental programming have focused on nutritional perturbations during the last two-thirds of gestation. Unfortunately, research investigating changes to maternal nutrient supply during early gestation or even periconceptually has been limited. The objectives of this invited review are to provide a summary of our current understanding of the impacts of inappropriate maternal nutrition during early gestation on embryo-fetal and offspring developmental outcomes. Nutrient supply to the conceptus varies during the time of early placental and conceptus development, and uterine glandular secretions, often called uterine milk or uterine histotroph, are major suppliers of nutrients during this time. Nutrients in histotroph are essential to conceptus development, and histotroph nutrient concentrations may be responsive to maternal nutrient supply. As the placenta develops and becomes fully functional, fetal nutrient supply becomes dependent on placental function. Research investigating changes in total maternal nutrient supply, even during early gestation, has clearly demonstrated impacts on offspring outcomes. Investigations into supply of specific nutrients and development outcomes have increased rapidly in recent years with results demonstrating that in some cases, even small changes in supply can have consequences on offspring development. Underlying mechanisms of developmental programming are associated with epigenetic events partially driven by methyl donors and cofactors. Emerging knowledge associated with the influence of periconceptual, fetal, and neonatal nutrition on offspring development, postnatal health, function, and productivity is creating opportunities for enhanced livestock production through strategic nutrient supplementation.
424 WS Young Scholar Talk PHD: Effect of increasing dietary zinc during late gestation and early lactation on ewe and lamb body weights, serum and milk minerals, and somatic cell count
Authors: Page Chad M 
Published: November 2020

Abstract
The objectives of the current research are to quantify the effects that increased dietary Zn during late gestation and early lactation has on ewe and progeny body weights, serum and milk minerals, and somatic cell count. Within Rambouillet (WF) and Hampshire (BF) breeds, ewes were ranked by BW and randomly assigned down the rank into 1 of 2 treatment groups: Control (n = 34, 37 mg Zn/kg DM, ≈1×NRC) and Zn treatment (n = 37, 113 mg Zn/kg DM, ≈3×NRC). Treatments were delivered via a ZnSO4-fortified alfalfa pellet fed at a rate of 0.45 kg/d DM from a RFID-activated automated feeder from d 108 ± 10 of gestation to d 30 post-lambing. Ewe BW were recorded at d 0, 29, lambing, 30 post-lambing, and weaning. Lamb BW was recorded at lambing, d 15, 30, and weaning. Serum samples were taken from ewes and lambs at 18 ± 4 h post-lambing and analyzed for mineral concentrations. Maternal trace mineral transfer efficiency were calculated by dividing lamb serum values by their respective dam’s serum value and expressed as a percentage. Ewe milk was collected twice weekly. Milk was analyzed for mineral content (d 0, 10, and 30 of lactation) and SCC (d 3–5, 6–9, 10–12, 13–16, 17–19, 20–23, 24–26, 27–29, or 30–32). Ewe and lamb BW was not influenced by Zn treatment (P &gt; 0.19). Ewe serum Zn and maternal transfer efficiency did not differ between control and Zn treatment ewes (P ≥ 0.47). There was a treatment × breed type interaction for lamb serum LogZn (P = 0.04), where BF lambs within Zn treatment had greater serum LogZn (0.63 ± 0.32) than WF lambs (-0.37 ± 0.27; P = 0.04), but breeds did not differ within lambs in the Control treatment group. Milk Ni and Zn was greater for Zn treated ewes than control ewes (P &lt; 0.01), but Mg and P concentrations were greater for control ewes (P ≤ 0.02). Control and Zn treated ewes did not differ in LogSCC through the first 30 d of lactation (P = 0.68) nor did they differ at weaning (P = 0.48). White face and BF ewes LogSCC did not differ at weaning (P = 0.09), but for the first 30 d of lactation BF (5.79 ± 0.06) had greater LogSCC than WF ewes (5.54 ± 0.06; P &lt; 0.01). Day of lactation impacted ewe SCC (P &lt; 0.01), with peak SCC between d 6 and 9 which began to decline as lactation progressed. Current Zn recommendations appear to be adequate for ewe and lamb growth during late gestation and early lactation, but results suggest litter size and breed nuances. Milk Zn is also increased with dietary Zn above NRC recommendations, while further interactions with milk Ni, Mg, and P occur. Additionally, longitudinal values of SCC throughout lactation may inform preventative intervention strategies for cases of sub-clinical mastitis since peak SCC is within the first 9 d post-lambing.
279 Development, selection criteria, and performance of Composite IV sheep at the U.S. Meat Animal Research Center
Authors: Murphy Tom , Freking Brad , Bennett Gary L , Keele John W 
Published: November 2020

Abstract
Average ewe prolificacy is low across the U.S., while fixed and variable costs of production can be substantial, especially in traditional shed-lambing systems. Pasture-lambing can reduce input costs but can present greater risk of inclement weather, predation, and internal parasitism. The Composite IV is a ½ Romanov, ¼ Katahdin, and ¼ White Dorper hair sheep developed at the U.S. Meat Animal Research Center. Objectives were to summarize Composite IV ewe and lamb performance under a pasture-lambing system with minimal disruption through weaning. Lamb survival to and body weight at weaning were greatest for singles (0.90 and 19.9 kg, respectively), intermediate for twins (0.82 and 15.9 kg), and lowest for triplets (0.65 and 14.6 kg; P &lt; 0.01). Number of lambs born and weaned per ewe lambing were lower at 1-yr of age (1.55 and 1.24, respectively) than at 3- to 5-yr of age (2.20 to 2.23 and 1.75 to 1.82; P &lt; 0.01). Within mature ewes (2- to 5-yr-old), twin litters were most frequent (58.7%), triplets intermediate (24.7%), and singles least (16.6%). While number of lambs weaned and total litter weaning weight were greater for triplet- than twin-bearing ewes (2.06 vs 1.70 and 28.9 vs 26.0 kg, respectively; P &lt; 0.01), triplet-bearing ewes lost 1.78 lambs per additional lamb weaned. Results suggest productivity in pasture-lambing systems can be enhanced by identifying complementary breed combinations, maintaining heterosis, and selecting for characteristics associated with reduced labor and enhanced well-being.