bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation of a bilayer edible film incorporated with lysozyme and its effect on fish spoilage bacteria
Authors: Li Qiuying , Xu Jinxiu , Zhang Dongdong , Zhong Keli , Sun Tong , Li Xuepeng , Li Jianrong 
Published: July 2020

AbstractAn edible bilayer film incorporated with lysozyme based on chitosan and sodium alginate was prepared via the layer‐by‐layer method. The film was characterized and its effects against the fish spoilage bacteria Pseudomonas fluorescens and Shewanella putrefaciens were investigated and compared to the monolayer films. The results suggest that electrostatic interactions such as hydrogen bonds occurred between the two layers of the bilayer film. Compared with monolayer films, the mechanical and gas barrier properties of the bilayer film were improved and higher inhibitory activity against both bacteria was exhibited. After treatment with the bilayer film, scanning electron microscopy revealed the serious damage to the bacterial cell surface, and cell membrane permeability and nucleic acid leakage increased. The bilayer film also affected the activity of alkaline phosphatase and adenosine triphosphatase. SDS‐PAGE of bacterial total protein revealed that the protein concentration n decreased, which may be due to the leakage of proteins from the damaged bacterial cell. All the results indicate that the bilayer film possesses good physicochemical properties and can destroy the bacterial cell membrane. Such superior antibacterial properties against fish spoilage bacteria indicate a good potential application of bilayer films in fish preservation.
Variation in SARS‐CoV‐2 Prevalence in U.S. Skilled Nursing Facilities
Authors: White Elizabeth M. , Kosar Cyrus M. , Feifer Richard A. , Blackman Carolyn , Gravenstein Stefan , Ouslander Joseph , Mor Vincent 
Published: July 2020

OBJECTIVETo identify county and facility factors associated with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) outbreaks in skilled nursing facilities (SNFs).DESIGNCross‐sectional study linking county SARS‐CoV‐2 prevalence data, administrative data, state reports of SNF outbreaks, and data from Genesis HealthCare, a large multistate provider of post‐acute and long‐term care. State data are reported as of April 21, 2020; Genesis data are reported as of May 4, 2020.SETTING AND PARTICIPANTSThe Genesis sample consisted of 341 SNFs in 25 states, including a subset of 64 SNFs that underwent universal testing of all residents. The non‐Genesis sample included all other SNFs (n = 3,016) in the 12 states where Genesis operates that released the names of SNFs with outbreaks.MEASUREMENTSFor Genesis and non‐Genesis SNFs: any outbreak (one or more residents testing positive for SARS‐CoV‐2). For Genesis SNFs only: number of confirmed cases, SNF case fatality rate, and prevalence after universal testing.RESULTSOne hundred eighteen (34.6%) Genesis SNFs and 640 (21.2%) non‐Genesis SNFs had outbreaks. A difference in county prevalence of 1,000 cases per 100,000 (1%) was associated with a 33.6 percentage point (95% confidence interval (CI) = 9.6–57.7 percentage point; P = .008) difference in the probability of an outbreak for Genesis and non‐Genesis SNFs combined, and a difference of 12.5 cases per facility (95% CI = 4.4–20.8 cases; P = .003) for Genesis SNFs. A 10‐bed difference in facility size was associated with a 0.9 percentage point (95% CI = 0.6–1.2 percentage point; P < .001) difference in the probability of outbreak. We found no consistent relationship between Nursing Home Compare Five‐Star ratings or past infection control deficiency citations and probability or severity of outbreak.CONCLUSIONSLarger SNFs and SNFs in areas of high SARS‐CoV‐2 prevalence are at high risk for outbreaks and must have access to universal testing to detect cases, implement mitigation strategies, and prevent further potentially avoidable cases and related complications. J Am Geriatr Soc 68:2167–2173, 2020.
Outbreak of Phytophthora cinnamomi causing severe decline of avocado trees in southern Turkey
Authors: Kurbetli İlker , Sülü Görkem , Aydoğdu Mehmet , Woodward Stephen , Bayram Süleyman 
Published: July 2020

AbstractSince the summer 2017, severe decline symptoms have been observed on 10‐ to 25‐year‐old avocado trees in almost all commercial orchards planted in the Mediterranean coastal region of Turkey. Young, newly planted trees in infected orchards were also affected by the disease. Affected trees showed wilting, leaf discoloration, defoliation and severe dieback. Some trees were completely desiccated. Although fine roots of symptomatic trees usually were decayed, reddish brown cankers also occurred on taproots and lateral roots of heavily infected trees. The pathogens were isolated from necrotic root and soil samples of symptomatic trees, using selective medium and soil baiting, and were identified based on morphological features and DNA sequences of the internal transcribed spacer (ITS) region. One isolate each of Phytophthora cryptogea and P. palmivora was identified, while all other isolates were P. cinnamomi. In addition, a subcortical fan‐shaped mycelium, characteristic of Armillaria spp., was observed in the stem base of a symptomatic tree and identified as Armillaria gallica by DNA sequences of the internal transcribed spacer (ITS) and the translational elongation factor 1‐α (EF 1‐α) gene regions. Pathogenicity of Phytophthora isolates was tested by stem inoculation on one‐year‐old avocado seedlings. Two months after inoculation, canker lesions developed on stems of seedlings inoculated by any of the three Phytophthora spp. In contrast, collenchyma callus formed over the wound points on control plants over the same time period. This is the first report of P. cinnamomi, P. cryptogea, P. palmivora and A. gallica causing root rot of avocado trees in Turkey. In addition, P. cryptogea and A. gallica are reported for the first time associated with disease on this host. Due to the severe symptoms and widespread occurrence, P. cinnamomi should be considered a potential threat to avocado cultivation and natural ecosystems of this region of Turkey.
Behavioural sleep in two species of buccal pumping sharks (Heterodontus portusjacksoni and Cephaloscyllium isabellum)
Authors: Kelly Michael L. , Spreitzenbarth Stefan , Kerr Caroline C. , Hemmi Jan M. , Lesku John A. , Radford Craig A. , Collin Shaun P. 
Published: July 2020

AbstractSleep is known to occur in most, if not all, animals studied thus far. Recent studies demonstrate the presence of sleep in flatworms and jellyfish, suggesting that this behaviour evolved early in the evolution of animals. Sharks are the earliest known extant, jawed vertebrates and may play an important role in understanding the evolutionary history of sleep in vertebrates, and yet, it is unknown whether they sleep. The Port Jackson (Heterodontus portusjacksoni) and draughtsboard (Cephaloscyllium isabellum) sharks are both benthic, buccal pumping species and remain motionless for extended periods of time. Whether these periods of prolonged inactivity represent sleep or quiet wakefulness is unknown. A key criterion for separating sleep from other quiescent states is an increased arousal threshold. We show here that inactive sharks of both species require significantly higher levels of electric stimulation before they show a visible response. Sharks deprived of rest, however, show no significant compensatory increase in restfulness during their normal active period following enforced swimming. Nonetheless, increased arousal thresholds in inactive animals suggest that these two species of shark sleep – the first such demonstration for members of this group of vertebrates. Further research, including electrophysiological studies, on these and other sharks, is required for a comprehensive understanding of sleep in cartilaginous fishes.
Ameliorative effect of Lantana camara biochar on coal mine spoil and growth of maize (Zea mays)
Authors: Ghosh Dipita , Masto Reginald Ebhin , Maiti Subodh Kumar 
Published: July 2020

AbstractThe aftermath of surface mining is a wasteland deprived of vegetation, soil structure and biodiversity. The unearthed overburden material is nutrient deprived and can only support the growth of invasive weeds such as Lantana camera which often cause allelopathy. The aim of the study is to prepare biochar from these noxious weeds and use it as an amendment for the mine spoil reclamation. Lantana biochar (LB) was prepared and applied to mine spoil, and Zea mays L. growth on biochar amended mine spoil was monitored for three months. Biochar application in a coal mine spoil using LB is comparatively de novo approach for reclamation practitioners. LB was prepared at varying temperature (250, 350 and 450℃) and residence times (30, 45 and 60 min) and characterized. After characterization, the most recalcitrant biochar at 450℃ for 60 min was chosen for application for the study. A pot trial was conducted to study the effect of LB at 0, 5, 10, 20 and 30 g kg−1 dosage on the yield of Zea mays and mine spoil properties. Significant ameliorative effects were observed with increase in organic carbon content (2.9 times), cation exchange capacity (2 times), water holding capacity (0.13 times) and decrease in bulk density (0.5 times) in the mine spoil. The seedling vigour index and germination also increased significantly (p < .05) at 30 g kg−1 biochar treatment compared to control. The study concluded that LB has the potential to remediate coal mine spoils and promote re‐vegetation in degraded land.
In-situ observation of damage evolution in polycarbonate subjected to hypervelocity impact
Authors: Kawai Nobuaki , Nagano Mikio , Hasegawa Sunao , Sato Eiichi 
Published: July 2020

Abstract
In the fields of space engineering and planetary science, hypervelocity impact phenomena have been studied as they relate to the space debris problem and planetary impact. With regard to hypervelocity-impact-induced damage, many studies focus on the evaluation of impact-damage geometry and morphology, for example, to construct the ballistic limit equations and/or penetrating equations for space structures, and to predict the size and shape of crater and fragments generated by planetary impact [1-4]. While the final state or late stage of an impact event are of primal interest, damage accumulation at early stages affect the overall outcome of the impact event. The understanding of hypervelocity-impact-damage processes lead to improvement of material-response models for hypervelocity impact and higher fidelity simulations of hypervelocity impact events. Under such a background, we have performed real-time imaging of hypervelocity-impact events on transparent materials to investigate the impact-damage formation and evolution processes [5-7]. In our previous work, the stress-wave-propagation behavior and damage evolution were observed by means of a transmitted light shadowgraph. In these measurements, the shape of the longitudinal-stress-wave front, crater and spall fracture were successfully visualized. On the other hand, these shadowgraph images provide little information about damage microstructure. The shadowgraph has difficulty in visualizing ramped waves, such as the release wave, and also for the shear wave which is not accompanied by the change of volumetric strain. Those play important role in initiating damage. This occurs because the intensity of the shadowgraph image depends on the second spatial derivative of the refractive index. In this study, we try two types of real-time imaging of impact events. One is imaging by using scattered light on the impacted target to visualize the microstructure of the impact-induced damage, the other is a shadowgraph using polarized light to visualize propagation of the impact-induced stress field.
Shear band characteristics in high strain rate naval applications
Authors: Conway Michael J. P. , Hogan James D. 
Published: July 2020

Abstract
This paper explores the dynamic behavior of HSLA 65 naval steels, specifically focusing on the initiation and growth of shear bands in quasi-static and dynamic compression experiments and how these bands affect stress-strain responses. The results indicate that the yield strength for this HSLA 65 increases from 541 ± 8 MPa for quasi-static (10-3 s-1) to 1081 ± 48 MPa for dynamic rates 1853 ± 31 s-1, and the hardening exponent increases from 0.376 ± 0.028 for quasi-static to 0.396 ± 0.006 for dynamic rates. Yield behavior was found to be associated with the onset of shear banding for both strain-rates, confirmed through visualization of the specimen surface using high-speed and ultra-high-speed cameras. For the quasi-static case, shear banding and yielding was observed to occur at 2.5% strain, and were observed to grow at speeds of upwards of 38 mm/s. For the dynamic experiments, the shear banding begins at approximately 1.18 ± 0.06% strain and these can grow upwards of 2122 ± 213 m/s during post-yield softening. Altogether, these measurements are some of the first of their kind in the open literature, and provide guidance on the critical time and length scales in shear banding. This information can be used in the future to design more failure-resistant steels, which has broader applications in construction, defense, and natural resource industries.
Voitenko experiments with novel diagnostics detect velocities of 89 km/s
Authors: Tasker Douglas G. , Bae Young K. , Johnson Carl , Rainey Kevin , Campbell Christopher , Oschwald Dave , Reed Cornelius 
Published: July 2020

Abstract
Using a Voitenko accelerator [1-3], a series of experiments were performed with the goal of attaining shock velocities in gases approaching 90 km/s. Typically, the basic apparatus comprises a hemispherical bowl filled with a gas at atmospheric pressure; a metal piston across its diameter; and a small bore evacuated shock tube at its apex, Fig. 1. The evacuated shock tube is separated from the gas bowl by a thin diaphragm. A combination of a plane wave explosive lens and a high explosive pad accelerates the piston to a velocity of the order of 4 km/s and subsequently compresses the gas in the bowl. The thin diaphragm at the other end of the bowl then ruptures and the high pressure (shock compressed) gas escapes into the shock tube.
Thin film graded density impactors for high rate off-Hugoniot loading: Application to Ta strength
Authors: Brown J. L. , Adams D. P. , Alexander C. S. , Wise J. L. , Reinhart W. D. 
Published: July 2020

Abstract
Graded density impactors (GDIs) have long been of interest to provide off-Hugoniot loading capabilities for impact systems. We describe a new technique which utilizes sputter deposition to produce an approximately 40 μm-thick film containing alternating layers of Al and Cu. The thicknesses of the respective layers are adjusted to give an effective density gradient through the film. The GDIs were launched into samples of interest with a 2-stage light gas gun, and the resulting shock-ramp-release velocity profiles were measured over timescales of ~10 ns with a new velocimetry probe. Results are shown for the direct impact of the film onto a LiF window, which allows for the dynamic characterization of the GDI, as well as from impact onto a thin (~40 μm) sputtered Ta sample backed by a LiF window. These measurements were coupled into mesoscale numerical simulations to infer the strength of Ta at the high rate (107 s-1), and high pressure (1 MBar) conditions this unique capability provides. Initial results suggest this is a viable strength platform which fills a critical gap and aids in cross-platform comparisons with other high-pressure strength platforms.
Effects of Additional Body on Jet Velocity of Hyper-cumulation
Authors: Mengwen XU , Xin JIA , Zhengxiang HUANG 
Published: July 2020

Abstract
In this paper, with different cone angles of the liner, the effects of the additional body’s material, thickness, and diameter on the velocity are studied by AUTODYN-2D. The results show as follows: 1. The additional body can increase the top velocity of the jet but has little effect on the tail velocity. 2. When the cone angle is larger, the velocity increases more, about 36%, and the velocity gradient is greater at the head of the jet. 3. There is little influence of material yield strength, but there is a large correlation with density. With the increase of material density, the velocity and velocity gradient increase. 4. The larger the density of the additional body, the smaller the optimum thickness. When the thickness is not less than the optimum thickness, an increase in the diameter of the additional body can improve the velocity and stability of the jet. Consequently, this kind of hyper-cumulation structure with a flat plate additional body is more suitable to improve the classical liner with a large cone angle. By modifying the design of the shaped charge in this way, the velocity of the jet can be improved effectively, enhancing the penetration power compared with the classical one.
X-ray diffraction diagnostic paired with gas gun driven compression of polyethylene
Authors: Huber Rachel C. , Watkins Erik B. , Dattelbaum Dana M. , Gustavsen Richard L. 
Published: July 2020

Abstract
Understanding the kinetics of phase transitions, including decomposition from reactants to products under extreme condition events is challenging. Capturing these processes require: 1) diagnostics that probe on the timescales and at energies capable of interacting with the dynamically evolving products, penetrating the opaqueness of the changing system; and 2) detectors sensitive enough to observe these events. Synchrotrons and free electron lasers provide ke-V-energy x-ray beams capable of penetrating the optical-opaqueness of the temporally evolving products. At the Dynamic Compression Sector at the Advanced Photon Source, the x-ray beam is coupled to single and two-stage gas guns capable of producing planar shocks at a range of projectile velocities while capturing in situ x-ray diffraction/scattering of the evolving material under dynamic compression. In this work, we demonstrate the utility of this approach in measuring the evolution of crystalline domains in shocked high-density polyethylene to P = 7.45 GPa, and have observed the compression and orientation of the polymer chains in real time.
Primary photodynamic therapy for small amelanotic choroidal melanomas: consecutive case series of 69 patients with at least 24-month follow-up
Authors: Quhill Hibba , Gosling Daniel , Sears Katharine , Rundle Paul 
Published: July 2020

AimsTo investigate the success and recurrence rates and visual outcomes in a large case series of amelanotic posterior choroidal melanomas treated by means of primary photodynamic therapy (PDT) with verteporfin.MethodsRetrospective case series from a single specialist ocular oncology centre. All patients had a clinical diagnosis of choroidal melanoma and were selected for PDT based on tumour characteristics. Included patients had at least 24 months of follow-up from initiation of treatment and all but one had not received treatment prior to PDT.Results69 patients were included. Mean tumour thickness was 1.9 mm (range 0.5–4.4), while the mean basal diameter was 6.9 mm (range 2.4–11.0). Included lesions were stage cT1a (n=66) or cT2a (n=3). The mean duration of follow-up from treatment initiation was 57 months (range 24–116 months). Seven lesions (10%) failed to respond to PDT. 10 patients (16%) experienced recurrence during follow-up. Overall success rate in this series was 75% (n=52). 83% of successfully treated patients (n=43) maintained or gained vision by final follow-up. Visual outcomes were significantly better in those patients who received PDT therapy alone in comparison to those who needed other treatments for their melanoma (Fisher’s exact test, p=0.004). Unfortunately, one patient (1.4%) in the series developed systemic metastases and died.ConclusionSelected amelanotic posterior uveal melanomas may be successfully treated with PDT with retention of good vision in the majority of cases, maintained with a mean of 57 months (minimum of 24 months) of follow-up.
Detection of C-peptide in human hair and nail: a comparison between healthy persons and persons with type 1 diabetes
Authors: Salih Jamal M , Abdulateef Darya S 
Published: July 2020

ObjectivesSerum and urinary C-peptide has clinical implications in people with/without diabetes. Recently, C-peptide was detected in hair samples of healthy adults but not studied in people with diabetes. It is not known whether C-peptide can be detectable in nail tissue or not. This study aims to assess the detection of C-peptide in hair and nail samples and to find whether hair and nail C-peptide levels are different in type 1 diabetes mellitus (T1DM) compared with healthy individuals.Research design and methodsIn a prospective case-control study on 41 subjects with T1DM and 42 control subjects, hair and nail samples were collected and prepared. C-peptide was extracted by incubating the samples with methanol and measuring the extract with an immunoassay. The hair and nail C-peptide values were compared between the T1DM and control group and their correlations with each other and with other variables were assessed with a significant level set at 0.05.ResultsHair and nail C-peptide levels were detected in both groups, with significantly lower values in T1DM compared with the control group. T1DM with >7-year diabetes duration had significantly lower C-peptide in serum, nails and hair. Hair and nail C-peptide levels have significant positive correlations with each other and negative correlations with age.ConclusionsWe conclude that C-peptide are detectable in the hair and nails of healthy persons and persons with T1DM. Compared with the healthy persons, persons with T1DM had significantly lower hair and nail C-peptide and significant hair/nail C-peptide reduction after 7 years of diagnosis. Our results suggest that hair and nails are suitable matrices for the measurement of C-peptide in healthy persons and persons with T1DM.
Infiltrative tumour growth pattern correlates with poor outcome in oesophageal cancer
Authors: Anciaux Maelle , Demetter Pieter , De Wind Roland , Gomez Galdon Maria , Vande Velde Sylvie , Lens Gaspard , Craciun Ligia , Deleruelle Amélie , Larsimont Denis , Lenaerts Tom , Sclafani Francesco , Deleporte Amélie , Donckier Vincent , Hendlisz Alain , Vandeputte Caroline 
Published: July 2020

ObjectiveOesophageal cancer (OEC) is an aggressive disease with a poor survival rate. Prognostic markers are thus urgently needed. Due to the demonstrated prognostic value of histopathological growth pattern (HGP) in other cancers, we performed a retrospective assessment of HGP in patients suffering from invasive OEC.DesignA first cohort composed of 89 treatment-naïve operated patients with OEC from The Cancer Genome Atlas (TCGA) public database was constituted, from which H&E images and RNA-sequencing data were retrieved. Next, a second cohort composed of 99 patients with OEC treated and operated in a Belgian hospital was established. H&E-stained sections and extracted tumorous RNA were obtained from the samples. HGP were assessed on H&E slides as infiltrative (IGP) or expansive (EGP). TCGA RNA-sequencing data were analysed through the gene set enrichment analysis and Cytoscape softwares. Real-time quantitative PCR (qPCR) experiments were performed to assess gene expression in the Belgian cohort.ResultsIGP patients displayed a grim prognosis compared with EGP patients, while IGP was found as associated with numerous lymphovascular emboli and perinervous infiltrations. Analyses of the TCGA expression data showed that angiogenesis, epithelial-to-mesenchymal transition (EMT) and inflammation were significantly upregulated in IGP compared with EGP samples. qPCR experiments of three genes appearing as highly upregulated in each pathway showed no difference in expression according to the HGP.ConclusionThe current study demonstrates the poor prognostic value carried by IGP in OC and suggests angiogenesis, EMT and inflammation as key carcinogenetic pathways upregulated in this pattern.