bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluasi Penyimpanan Obat di Gudang Farmasi RSUD Ratu Zalecha Martapura
Authors: Lestari Octa Linda , Kartinah Nani , Hafizah Noor 
Published: November 2020

Penyimpanan adalah kegiatan untuk menghindari obat dari kerusakan baik fisik dan kimia serta memastikan kualitas obat tetap terjamin. Penelitian ini bertujuan untuk mengevaluasi penyimpanan obat di Instalasi Gudang Farmasi RSUD Ratu Zalecha Martapura berdasarkan indikator USAID. Penelitian bersifat deskriptif non-eksperimental dengan rancangan penelitian cross sectional dan pengambilan data dilakukan secara prospektif. Indikator yang dievaluasi yaitu tingkat akurasi persediaan obat, tingkat akurasi penempatan obat, tingkat akurasi pengambilan obat, waktu pemrosesan permintaan obat, pemanfaatan ruang penyimpanan, serta tingkat keamanan di lokasi penyimpanan menurut USAID. Hasil penelitian menunjukkan persentase tingkat akurasi persediaan obat 100%, persentase tingkat akurasi penempatan obat sebesar 85%, dan persentase tingkat akurasi pengambilan obat 97%. Waktu yang diperlukan oleh petugas gudang dalam pemrosesan permintaan obat yaitu 3 - 66 menit, persentase pemanfaatan gudang untuk menyimpan obat sebesar 43%, dan terdapat Standar Prosedur Operasional terkait tingkat keamanan obat di gudang penyimpanan obat. Dapat disimpulkan bahwa hasil evaluasi berdasarkan indikator USAID menunjukkan penyimpanan obat di Gudang Farmasi RSUD Ratu Zalecha Martapura masih belum efisien karena masih terdapat indikator yang belum memenuhi standar yaitu indikator tingkat akurasi penempatan obat dan tingkat akurasi pengambilan obat. Storage of medicine is an activity to prevent drugs from physical and chemical damage and to guarantee the quality of drugs. This study aims to evaluate the drug storage at the Installation of Pharmacy in Ratu Zalecha Martapura Hospital based on USAID indicators. This research is a descriptive non-experimental study with a cross-sectional study and prospectively data. The indicators evaluated are the accuracy of drug supplies, the accuracy of drug placement, the accuracy of drug collection, the processing time for drug requests, the utilization of storage space, and the level of security at storage locations according to USAID. The results showed an accurate rate of drug supplies was 100%, percent of the accuracy rate of drug placement was 85%, and percent of the accuracy of drug-taking was 97%. The time required by warehouse officers in processing drug requests is 3 - 66 minutes, the percentage of warehouse used to store drugs is 43%, and there are Standard Operating Procedures related to the safety level of drugs in drug storage warehouses. The conclusion of this research is the evaluation based on USAID indicators show that drug storage in the Pharmacy Warehouse of the Ratu Zalecha Martapura Hospital is still inefficient because there are still indicators that do not meet the standards, namely indicators of the accuracy of drug placement and the accuracy of drug-taking.Keywords : Drug Storage, Warehouse, USAID Indicator, Pharmaceutical Managemen
Analisis Pengaruh Penggunaan Depot Medroxyprogesterone Acetate Terhadap Kenaikan Berat Badan Akseptor di Puskesmas Perumnas II Pontianak
Authors: Nurmainah Nurmainah , Wahdaningsih Sri , Innas Syaazaratul Qamelia 
Published: November 2020

Kontrasepsi suntik merupakan salah satu kontrasepsi hormonal yang masih menjadi pilihan akseptor dalam mengatur kehamilan. Namun demikian, penggunaan kontrasepsi suntik Depot Medroxyprogesterone Acetate (DMPA) diketahui dapat meningkatkan berat badan selama pemakaian enam (6) bulan atau lebih. Tujuan penelitian ini dilakukan untuk menganalisis pengaruh penggunaan DMPA terhadap kenaikan berat badan akseptor. Penelitian ini merupakan penelitian observasional dengan rancangan penelitian potong lintang (cross sectional) yang bersifat analitik. Populasi dalam penelitian ini adalah seluruh akseptor baru suntik DMPA di Puskesmas Perumnas II Pontianak pada bulan Januari 2018 hingga Maret 2019. Variabel dari penelitian ialah usia, pekerjaan, paritas, dan kenaikan berat badan. Analisis dilakukan dengan menggunakan analisis paired t-test. Teknik pengambilan sampel yang digunakan ialah purposive sampling. Jumlah sampel yang memenuhi kriteria inklusi dan ekslusi sebanyak 81 akseptor. Dari hasil penelitian ini diperoleh bahwa sebagian besar akseptor berusia 20-35 tahun (71,6%), bekerja sebagai ibu rumah tangga (97,5%), mempunyai 2 anak atau lebih (77,8%), dan memiliki kenaikan berat badan 0-2 kg (44,4%). Berdasarkan hasil analisis paired t-test bahwa penggunaan suntik KB 3 bulan (DMPA) memiliki pengaruh terhadap kenaikan berat badan akseptor dengan nilai p=0,001. Kesimpulan dari penelitian ini bahwa terdapat pengaruh penggunaan DMPA terhadap kenaikan berat badan akseptor. Injectable contraception is a hormonal contraceptive used by acceptors in regulating pregnancy. However, the use of Depot Medroxyprogesterone Acetate (DMPA) is known to increase body weight after six months of use or more.. The purpose of this study was to analysis the effect of using DMPA on weight gain. This study was an observational study with cross sectional analytic study design. The population in this study were all new acceptors who used DMPA at the Perumnas II Pontianak Public Health Care in January 2018 to March 2019. Variables from the study were age, occupation, parity, and weight gain. Analysis was performed using paired t-test analysis. The sampling technique used was purposive sampling, where the number samples that met the inclusion and exclusion criteria were 81 acceptors. The results showed that most of the acceptors were aged 20-35 years (71,6%), work as housewives (97,5%), had 2 or more children (77,8%), and gained weight 0-2 kg (44,4%). Based on the results of paired t-test analysis, the use of DMPA has an effect on the acceptor’s weight gain with a value of p = 0.001. The conclusion of this study is that there is an effect of the use of DMPA on acceptor weight gain.Keywords: Acceptors, DMPA, weigt gain
Aktivitas Antioksidan Ekstrak Etanol Muntingia calabura dengan Variasi Laju Pengadukan Menggunakan Macerator-Magnetic Stirrer (M-MS)
Authors: Zaini Muhammad , Hidriya Hajrah , Japeri Japeri 
Published: November 2020

Maserasi merupakan salah satu metode ekstraksi yang digunakan untuk menarik kandungan kimia dari bahan alam. Macerator Magnetic Stirrer (M-MS) merupakan alat yang dikembangkan untuk memaksimalkan proses maserasi melalui pengadukan secara berkesinambungan. Tujuan penelitian ini adalah untuk mengetahui efektivitas penggunaan M-MS dalam proses ekstraksi senyawa bahan alam. Parameter yang digunakan yaitu persentase zat terekstraksi (% rendemen) dan aktivitas antioksidan (IC50) ekstrak etanol Muntingia calabura (EEMC) menggunakan metode DPPH (2,2-Diphenyl-1-Picrylhydrazil). Pengujian dilakukan terhadap simplisia daun Muntingia calabura yang dimaserasi selama 24 jam menggunakan etanol 95% dengan maserator konvensional, M-MS dengan laju pengadukan 200 rpm dan 300 rpm. Pengadukan dengan maserator konvensional dilakukan sebanyak 3 kali setiap 8 jam, sedangkan dengan M-MS dilakukan secara kontinyu selama 24 jam. Nilai % rendemen EEMC dari maserator konevnsional, M-MS 200 rpm dan 300 rpm secara berturut-turut adalah 7,6 %, 8,4 % dan 10,2 %. Hasil uji aktivitas antioksidan menghasilkan nilai IC50 EEMC dengan maserator konvensional sebesar 18,19 ppm, M-MS 200 rpm adalah 14,35 ppm dan 300 rpm adalah 7,85 ppm. Nilai IC50 untuk masing-masing uji menunjukan < 50 ppm yang termasuk dalam kategori sangat kuat. Ekstraksi EEMC paling efektif adalah menggunakan M-MS dengan laju pengadukan 300 rpm. The maceration is one of the extraction methods used to extract chemical content from natural compounds. Macerator Magnetic Stirrer (M-MS) is a tool developed to maximize the maceration process through continuous stirring. The purpose of this study was to determine the effectiveness of using M-MS in the process of extracting natural compounds. The parameters used were the percentage of extracted substance (% yield) and antioxidant activity (IC50) of ethanol extract of Muntingia calabura (EEMC) using the DPPH method (2,2-Diphenyl-1-Picrylhydrazil). Tests were carried out on the simplicia of Muntingia calabura leaves which were macerated for 24 hours using 95% ethanol with a conventional macerator, M-MS with a stirring rate of 200 rpm and 300 rpm. Stirring with a conventional macerator was carried out 3 times every 8 hours, while with M-MS it was done continuously for 24 hours. EEMC yield value respectively of conventional macerator, M-MS at 200 rpm and 300 rpm showed 7.6 %, 8.4% and 10.2 %. The results of the antioxidant activity test resulted in the IC50 EEMC value with a conventional macerator is 18.19 ppm, M-MS 200 rpm is 14.35 ppm and 300 rpm is 7.85 ppm. The IC50 value for each test shows <50 ppm which is included in the very strong category. The most effective EEMC extraction is using M-MS with a stirring rate of 300 rpm.Keywords: maceration, M-MS, EEMC, antioxidant
Rios de Marielle: uma experiência de formação de professores/as entre direitos humanos, patrimônio e cidade
Authors: Costa Carina Martins 
Published: November 2020

O artigo apresenta uma experiência desenvolvida na disciplina Estágio Supervisionado, do curso de Licenciatura em História (UERJ), baseado no uso da cidade e do patrimônio da cidade do Rio de Janeiro. O ensino de Direitos Humanos foi o eixo central pela abordagem das memórias e esquecimentos em torno da vereadora assassinada Marielle Franco (PSOL/RJ) e das lutas em prol da defesa de igualdade de gênero, educação antirracista e direito à cidade. A metodologia utilizada foi a pesquisa-ação, com a proposição de roteiros pedagógicos, materiais didáticos e performances, na perspectiva sócio-histórica de ensino-aprendizagem. Foram acionadas políticas públicas brasileiras sobre Direitos Humanos e formação de professores/as, teóricos da área e a produção acadêmica de Marielle Franco. Por fim, o artigo pretendeu analisar algumas produções culturais e refletir sobre a avaliação do processo formativo realizada pelos/as professores/as em formação. Palavras-chave: Direitos Humanos, Formação de professores, Educar na cidade, Patrimônio
How does the self-reported health of undergraduate nursing students change during their degree programme? Survey results from a Scottish University
Authors: Evans Josie , Andreis Federico , Cameron Dawn M , Eades Claire E 
Published: November 2020

Abstract
Background: The lifestyle behaviours, and the physical and mental health of nurses, are poorer than those of other allied health professionals, and of the general population. However, these were no less favourable among first year undergraduate nursing students at a Scottish Higher Education Institution (HEI) than among similar people of the same age. We compared health and health behaviours among the same cohort of undergraduate nursing students over the course of their degree. Methods: An anonymous self-complete cross-sectional survey was administered during a timetabled teaching session at three time-points to undergraduate nursing students at the start of Years 1, 2 and 3 of their programme. They had received written information about the study previously. Results: Self-reported health did not change significantly over time, but there was a decline over the three years in the proportions of students rating their mental health as excellent/very good/good and a concomitant increase in those rating their mental health as fair/poor. Correspondingly, the mean WEMWBS wellbeing score declined over the three years, with the proportion of students with a score of <46 (indicating either high risk of major depression, or in high risk of psychological distress and increased risk of depression) increasing from one quarter to one half. This effect was captured and described using a Bayesian regression analysis. The most noticeable change in health behaviours was a decline in physical activity levels over the study period. The proportion of students managing 150 minutes of weekly physical activity decreased from three quarters to two thirds. This was reflected in higher self-reported sedentary time, although there were no observable trends over time in mean BMI, or proportions of students categorised as overweight or obese. Conclusions: This paper suggests that there may be a decline in mental health and in participation in physical activity among nursing students over the course of their degree. We recommend the incorporation of an intervention into the undergraduate nursing curriculum that promotes and encourages regular physical activity, offering students the opportunity to learn about health promotion and lifestyle change in practice, to improve their own physical health, and to address mental wellbeing.
The Impact of Irregular Corneal Shape Parameters on Visual Acuity and Contrast Sensitivity
Authors: Liduma Sanita , Luguzis Artis , Krumina Gunta 
Published: November 2020

Abstract
Background: The image optical quality is affected by changes in corneal shape of patients with keratoconus. The goal of this study was to explore which corneal parameters determine the visual quality in keratoconus subjects, which corneal slope parameter has the strongest correlation with visual quality and contrast sensitivity. Methods: The study covered eyes of 77 subjects, graded from the first to third keratoconus stages. To characterize the shape of cornea, we obtained measurements in two ways: (a) projected two perpendicular axes onto a cornea – the main axis passed through the central point of the cornea (visual axis projection) and keratoconus apex, while the second axis was perpendicular to the main axis – and read elevation values at points on theses axis; (b) projected circles with different diameters around the central part of the cornea (1, 2 and 3 mm) and read elevation values at points equally displaced on these circles. The measurements were used to calculate various elevation change (slope) parameters. Results: According to the acquired data, the visual acuity of a corrected eye does not have a strong correlation with the measured keratoconus apex slope. Contrast sensitivity displayed a strong correlation with keratoconus slope in the central part of the cornea (with a radius of 1 mm). Correlations in different spatial frequencies ranging from 0.47 to 0.6. Conclusion: Contrast sensitivity is more important parameter which describes the visual quality of keratoconus subjects than visual acuity. The most important region which determines the visual quality in keratoconus subjects is the region with a 1 mm radius of the corneal centre in the opposite direction of keratoconus apex.
SMARCA4 deficient tumours are vulnerable to KDM6A/UTX and KDM6B/JMJD3 blockade
Authors: Romero Octavio , Vilarrubi Andrea , Alburquerque-Bejar Juan , Gomez Antonio , Andrades Alvaro , Trastulli Deborah , Pros Eva , Verdura Sara , Farre Lourdes , Martin-Tejera Juan , Llabata Paula , Oaknin Ana , Saigi Maria , Piulats Josep , Matias-Guiu Xavier , Medina Pedro , Vidal August , Villanueva Alberto , Sanchez-Cespedes Montse 
Published: November 2020

Abstract
Despite the genetic inactivation of SMARCA4, a core component of the SWI/SNF-complex commonly found in cancer, there are no therapies that effectively target SMARCA4-deficient tumours. Here, we show that, unlike the cells with activated MYC oncogene, cells with SMARCA4 inactivation are refractory to the histone deacetylase inhibitor, SAHA, leading to the aberrant accumulation of H3K27me3. This is associated with impaired transactivation and significantly reduced levels of the histone demethylases KDM6A/UTX and KDM6B/JMJD3, and with a strong dependency on these histone demethylases, so that its inhibition compromises cell viability in the SMARCA4-mutant cells. Administering the KDM6 inhibitor GSK-J4 to mice orthotopically implanted with SMARCA4-mutant lung cancer cells or primary small cell carcinoma of the ovary, hypercalcaemic type (SCCOHT), had strong anti-tumour effects. Our results highlight the vulnerability of KDM6 inhibitors as a characteristic that could be exploited for treating SMARCA4-mutant cancer patients.
Transcription factor network analysis identifies REST/NRSF as an intrinsic regulator of CNS regeneration
Authors: Cheng Yuyan , Yin Yuqin , Zhang Alice , Bernstein Alexander , Kawaguchi Riki , Gao Kun , Potter Kyra , Gilbert Hui-ya , Ao Yan , Ou Jing , Fricano-Kugler Catherine , Goldberg Jeffrey , Woolf Clifford , Sofroniew Michael , Benowitz Larry , Geschwind Daniel 
Published: November 2020

Abstract
The inability of neurons to regenerate long axons within the CNS is a major impediment to improving outcome after spinal cord injury, stroke, and other CNS insults. Recent advances have uncovered an intrinsic program that involves coordinate regulation by multiple transcription factors that can be manipulated to enhance growth in the peripheral nervous system. Here, we used a system-genomics approach to characterize regulatory relationships of regeneration-associated transcription factors, identifying RE1-Silencing Transcription Factor (REST; Neuron-Restrictive Silencer Factor, NRSF) as a predicted upstream suppressor of a pro-regenerative gene program associated with axon regeneration in the CNS. We validate our predictions using multiple paradigms, showing that mature mice bearing cell type-specific deletions of REST or expressing dominant-negative mutant REST showed improved regeneration of the corticospinal tract and optic nerve, accompanied by upregulation of regeneration-associated genes in cortical motor neurons and retinal ganglion cells, respectively. These analyses identify a novel role for REST as an upstream suppressor of the intrinsic regenerative program in the CNS and demonstrate the power of a systems biology approach involving integrative genomics and bio-informatics to predict key regulators of CNS repair.
Activation pathway of a G protein-coupled receptor uncovers conformational intermediates as novel targets for allosteric drug design
Authors: He Xinheng , Yang Zhao , Chai Zong-Tao , Wang Junyan , Rehman Ashfaq , Ni Duan , Pu Jun , Sun Jinpeng , Zhang Jian , Lu Shaoyong 
Published: November 2020

Abstract
G protein-coupled receptors (GPCRs) are the most frequent targets of approved drugs. A complete mechanistic elucidation of large-scale conformational transitions underlying the activation mechanisms of GPCRs is of critical importance for therapeutic drug development. Here, we utilized a combined computational and experimental framework that integrated extensive molecular dynamics simulations, Markov state models, and site-directed mutagenesis for investigating the conformational landscape of activation of the angiotensin II (AngII) type 1 receptor (AT1R), a prototypical class A GPCR. Our findings suggested a synergistic transition mechanism of AT1R activation. Importantly, a key intermediate state was found in the activation pathway. We discovered a novel “cryptic” binding site in the intracellular region of the receptor in the intermediate state. Furthermore, a bioluminescence resonance energy transfer analysis revealed the insensitivity of the endogenous AngII octapeptide agonist in the activation of the downstream Gq signaling and β-arrestin-mediated pathways, upon mutations of the predicted cryptic binding site, thereby suggesting an allosteric regulatory mechanism. Together, these findings not only provide a deeper understanding of AT1R activation at an atomic level, but also open potential avenues for the design of allosteric AT1R modulators. Consequently, this will provide a broad range of applications for GPCR biology, biophysics, and medicinal chemistry.
BEAR reveals that increased fidelity variants can successfully reduce the mismatch-tolerance of adenine but not cytosine base editors
Authors: Tálas András , Simon Dorottya , Kulcsár Péter , Varga Éva , Welker Ervin 
Published: November 2020

Abstract
Adenine and cytosine base editors (ABE, CBE) are designed to generate single base mutations in genes without necessarily generating DNA double-strand breaks and undesired indel mutations. However, the activity of base editors employing an inactive (dead) SpCas9 is generally low, which may be increased only at the expense of generating undesired indels by using a nickase SpCas9. We have increased the efficiency of dead base editors to a level comparable to that of nickase base editors by enriching cells labelled for efficient base editing using Base Editor Activity Reporter (BEAR), a plasmid-based, fluorescent tool. Furthermore, by exploiting the semi-high-throughput potential of BEAR, we have examined the applicability of increased fidelity variants to decrease Cas9-dependent off-target effects that revealed that CBE remains active on off-targets where increased fidelity mutations and/or mismatches decrease the activity of ABE, making the strategy of applying increased fidelity variants more beneficial for ABE than for CBE.
Online-Synchronized Clinical Simulation: An efficient teaching-learning option for the COVID-19 pandemic time and beyond.
Authors: Díaz-Guio Diego Andrés , Ríos-Barrientos Elena , Santillán-Roldan Pablo Andrés , Díaz-Gómez Ana Sofía , Ricardo-Zapata Alejandra , Mora-Martinez Santiago , Barrientos-Aguiñaga Adrián , Martínez-Elizondo Joel Alejandro , Arroyo-Romero Maria Nathalie , Rodríguez-Morales Alfonso J. 
Published: November 2020

Abstract
Introduction: A powerful methodology for teaching, learning, and researching is clinical simulation, which has positioned itself in recent decades in health science education. However, due to the COVID-19 pandemic, social distancing has forced institutions to leave simulation centers and make use of new alternatives that allow the continuation of educational programs through virtual environments.Methods: We carried out a before-and-after design study that used online-synchronized clinical simulation (OSCSim) in three Latin American clinical simulation centers (Colombia, Ecuador, and Mexico). The educational intervention included briefing, COVID-19 simulated cases, and structured debriefing through a meeting platform. We assessed the participants' learning and performance in diagnosis, treatment, and nontechnical skills for the management of patients with COVID-19. Satisfaction and learning perception were measured. The debriefing quality was evaluated from the student's perspective.Results: We had 106 participants. 46.2% from Colombia, 31.1% from Mexico, and 22.6% from Ecuador. A total of 51.9% were men, and the median age was 23 years (IQR: 22-26). A total of 34.9% were fourth-year students of medicine, 38% were fifth-year students, and 21.7% were anesthesia residents. Fourteen OSCSim sessions were performed; cognitive engagement was 43.4%, mainly interactive. A relationship was found between cognitive engagement, learning, situational awareness, and realism in the simulation. The quality of debriefing was ranked high by the participants, and they also agreed with the OSCSim methodology.Conclusion: OSCSim is an active and social learning activity that enables training and improvement of nontechnical skills and declarative knowledge about COVID-19 management. Nevertheless, it needs to be complemented in the procedural aspect in simulation centers.