bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain
Authors: Cordaro Marika , Siracusa Rosalba , Impellizzeri Daniela , D’ Amico Ramona , Peritore Alessio Filippo , Crupi Rosalia , Gugliandolo Enrico , Fusco Roberta , Di Paola Rosanna , Schievano Carlo , Cuzzocrea Salvatore 
Published: November 2019

Abstract
Background
Osteoarthritis is increasingly recognized as the result of a complex interplay between inflammation, chrondrodegeneration, and pain. Joint mast cells are considered to play a key role in orchestrating this detrimental triad. ALIAmides down-modulate mast cells and more generally hyperactive cells. Here we investigated the safety and effectiveness of the ALIAmide N-palmitoyl-d-glucosamine (PGA) in inflammation and osteoarthritis pain.

Methods
Acute toxicity of micronized PGA (m-PGA) was assessed in rats following OECD Guideline No.425. PGA and m-PGA (30 mg/kg and 100 mg/kg) were orally administered to carrageenan (CAR)-injected rats. Dexamethasone 0.1 mg/kg was used as reference. Paw edema and thermal hyperalgesia were measured up to 6 h post-injection, when also myeloperoxidase activity and histological inflammation score were assessed. Rats subjected to intra-articular injection of sodium monoiodoacetate (MIA) were treated three times per week for 21 days with PGA or m-PGA (30 mg/kg). Mechanical allodynia and motor function were evaluated at different post-injection time points. Joint histological and radiographic damage was scored, articular mast cells were counted, and macrophages were immunohistochemically investigated. Levels of TNF-α, IL-1β, NGF, and MMP-1, MMP-3, and MMP-9 were measured in serum using commercial colorimetric ELISA kits. One- or two-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons was used.

Results
Acute oral toxicity of m-PGA resulted in LD50 values in excess of 2000 mg/kg. A single oral administration of PGA and m-PGA significantly reduced CAR-induced inflammatory signs (edema, inflammatory infiltrate, and hyperalgesia), and m-PGA also reduced the histological score. Micronized PGA resulted in a superior activity to PGA on MIA-induced mechanical allodynia, locomotor disability, and histologic and radiographic damage. The MIA-induced increase in mast cell count and serum level of the investigated markers was also counteracted by PGA and to a significantly greater extent by m-PGA.

Conclusions
The results of the present study showed that PGA is endorsed with anti-inflammatory, pain-relieving, and joint-protective effects. Moreover, it proved that particle size reduction greatly enhances the activity of PGA, particularly on joint pain and disability. Given these results, m-PGA could be considered a valuable option in the management of osteoarthritis.
Intestinal parasitic infections and determinant factors among school-age children in Ethiopia: a cross-sectional study
Authors: Dessie Awrajaw , Gebrehiwot Tesfay Gebregzabher , Kiros Berihu , Wami Sintayehu Daba , Chercos Daniel Haile 
Published: November 2019

Abstract
Objective
This study aimed to determine the prevalence of intestinal parasitic infections and associated factors among school-age children in Sebeya primary school, northern Ethiopia, 2017.

Results
The prevalence of intestinal parasites in school-age children was (29.9%). A total of six parasites were detected in this study. E. histolytica/dispar 19.43% (82/422) and G. lamblia 8.29% (35/422) were the predominant ones. Unclean fingernail (AOR = 1.72), defecating in the open field (AOR = 2.82), and being barefooted (AOR = 1.72) were the determinant factors for intestinal parasitic infections. Frequently washing hands reduced the chance of infections by 68%. Furthermore, children in grade 1–4 and 5–6 had higher odds developing the infections than those in grade 7–8 (AOR = 8.21 and AOR = 2.50, respectively).
Performance of three rapid diagnostic tests for the detection of Cryptosporidium spp. and Giardia duodenalis in children with severe acute malnutrition and diarrhoea
Authors: Bitilinyu-Bangoh Joseph , Voskuijl Wieger , Thitiri Johnstone , Menting Sandra , Verhaar Nienke , Mwalekwa Laura , de Jong Daisy B. , van Loenen Merlin , Mens Petra F. , Berkley James A. , Bandsma Robert H. J. , Schallig Henk D. F. H. 
Published: November 2019

Abstract
Background
There is significant need for accurate diagnostic tools for Cryptosporidium spp. and Giardia duodenalis infections in resource limited countries where diarrhoeal disease caused by these parasites is often prevalent. The present study assessed the diagnostic performance of three commercially available rapid diagnostic tests (RDTs) based on faecal-antigen detection for Cryptosporidium spp. and/or G. duodenalis infections in stool samples of children admitted with severe acute malnutrition (SAM) and diarrhoea. An established multiplex PCR was used as reference test.

Methods
Stool samples from children with SAM and diarrhoea enrolled in a randomized controlled trial (registered at clinicaltrials.gov/ct2/show/NCT02246296) in Malawi (n = 175) and Kenya (n = 120) between December 2014 and December 2015 were analysed by a multiplex PCR for the presence of Cryptosporidium spp., G. duodenalis or Entamoeba histolytica parasite DNA. Cryptosporidium-positive samples were species typed using restriction fragment length polymorphism analysis. A sub-sample of the stool specimens (n = 236) was used for testing with three different RDTs. Diagnostic accuracy of the tests under evaluation was assessed using the results of PCR as reference standard using MedCalc software. Pearson Chi-square test and Fisher’s exact test were used to determine (significant) difference between the number of cryptosporidiosis or giardiasis cases found by PCR in Malawi and Kenya. The overall diagnostic accuracy of each RDT was calculated by plotting a receiver operating characteristic (ROC) curve for each test and to determine the area under the curve (AUC) using SPSS8 software.

Results
Prevalence of Cryptosporidium spp. by PCR was 20.0 and 21.7% in Malawi and Kenya respectively, mostly C. hominis. G. duodenalis prevalence was 23.4 and 5.8% in Malawi and Kenya respectively. E. histolytica was not detected by PCR. RDT testing followed the same pattern of prevalence. RDT sensitivities ranged for cryptosporidiosis from 42.9 to 76.9% and for G. duodenalis from 48.2 to 85.7%. RDT specificities ranged from 88.4 to 100% for Cryptosporidium spp. and from 91.2 to 99.2% for G. duodenalis infections. Based on the estimated area under the curve (AUC) values, all tests under evaluation had an acceptable overall diagnostic accuracy (> 0.7), with the exception of one RDT for Cryptosporidium spp. in Malawi.

Conclusions
All three RDTs for Cryptosporidium spp. and Giardia duodenalis evaluated in this study have a moderate sensitivity, but sufficient specificity. The main value of the RDTs is within their rapidness and their usefulness as screening assays in surveys for diarrhoea.
Significant artefactual noise in 90Y TOF-PET imaging of low specific activity phantoms arises despite increased acquisition time
Authors: Hesse Michel , Walrand Stephan 
Published: November 2019

AbstractVolumes of usual PET phantoms are about four to sixfold that of a human liver. In order to avoid count rate saturation and handling of very high 90Y activity, reported TOF-PET phantom studies are performed using specific activities lower than those observed in liver radioembolization.However, due to the constant random coincidence rate induced by the natural crystal radioactivity, reduction of 90Y specific activity in TOF-PET imaging cannot be counterbalanced by increasing the acquisition time. As a result, most 90Y phantom studies reported images noisier than those obtained in whole-body 18F-FDG, and thus advised to use dedicated noise control in TOF-PET imaging post 90Y liver radioembolization.We performed acquisitions of the Jaszczak Deluxe phantom in which the hot rod insert was only partially filled with 2.6 GBq of 90Y. Standard reconstruction parameters recommended by the manufacturer for whole-body 18F-FDG PET were used.Low specific activity setups, although exactly compensated by increasing the acquisition time in order to get the same number of detected true coincidences per millilitre, were impacted by significant noise. On the other hand, specific activity and acquisition time setup similar to that used in post 90Y liver radioembolization provided image quality very close to that of whole-body 18F-FDG.This result clearly discards the use of low specific activity phantoms intended to TOF-PET reconstruction parameter optimization. Volume reduction of large phantoms can be achieved by vertically setting the phantoms or by adding Styrofoam inserts.
Interaction between arbuscular mycorrhizal fungi and Bacillus spp. in soil enhancing growth of crop plants
Authors: Nanjundappa Anuroopa , Bagyaraj Davis Joseph , Saxena Anil Kumar , Kumar Murugan , Chakdar Hillol 
Published: November 2019

AbstractSoil microorganisms play an important role in enhancing soil fertility and plant health. Arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria form a key component of the soil microbial population. Arbuscular mycorrhizal fungi form symbiotic association with most of the cultivated crop plants and they help plants in phosphorus nutrition and protecting them against biotic and abiotic stresses. Many species of Bacillus occurring in soil are also known to promote plant growth through phosphate solubilization, phytohormone production and protection against biotic and abiotic stresses. Synergistic interaction between AMF and Bacillus spp. in promoting plant growth compared to single inoculation with either of them has been reported. This is because of enhanced nutrient uptake, protection against plant pathogens and alleviation of abiotic stresses (water, salinity and heavy metal) through dual inoculation compared to inoculation with either AMF or Bacillus alone.
Mental Health In Elite Athletes: Increased Awareness Requires An Early Intervention Framework to Respond to Athlete Needs
Authors: Purcell Rosemary , Gwyther Kate , Rice Simon M. 
Published: November 2019

AbstractThe current ‘state of play’ in supporting elite athlete mental health and wellbeing has centred mostly on building mental health literacy or awareness of the signs of mental ill-health amongst athletes. Such awareness is necessary, but not sufficient to address the varied mental health needs of elite athletes. We call for a new model of intervention and outline the backbone of a comprehensive mental health framework to promote athlete mental health and wellbeing, and respond to the needs of athletes who are at-risk of developing, or already experiencing mental health symptoms or disorders. Early detection of, and intervention for, mental health symptoms is essential in the elite sporting context. Such approaches help build cultures that acknowledge that an athlete’s mental health needs are as important as their physical health needs, and that both are likely to contribute to optimising the athlete’s overall wellbeing in conjunction with performance excellence. The proposed framework aims at (i) helping athletes develop a range of self-management skills that they can utilise to manage psychological distress, (ii) equipping key stakeholders in the elite sporting environment (such as coaches, sports medicine and high-performance support staff) to better recognise and respond to concerns regarding an athlete’s mental health and (iii) highlighting the need for specialist multi-disciplinary teams or skilled mental health professionals to manage athletes with severe or complex mental disorders. Combined, these components ensure that elite athletes receive the intervention and support that they need at the right time, in the right place, with the right person.
Use of Photovoice to engage stakeholders in planning for patient-centered outcomes research
Authors: Nault Connors Jill D. , Conley Marshall J. , Lorenz Laura S. 
Published: November 2019

Abstract
Background
Research is needed to inform patient and provider decisions about how to best care for patients who go to the emergency department with complaints of chest pain when their symptoms are due to anxiety rather than a heart problem. However, this research may not be a high priority due, in part, to a lack of awareness for the severity of anxiety symptoms and the impact of anxiety on peoples’ daily lives. In this commentary article, we highlight the use of Photovoice as a unique method to share patients’ lived experience of anxiety with providers, researchers, and health system leaders.

Main text
A brief background on Photovoice methods, the process of patient partner involvement in Photovoice, and the project’s Photovoice results (posters, photos and captions) is presented.

Conclusion
Photovoice achieved its intended effects of increasing awareness of all stakeholders about the burden of anxiety in patients’ lives and the imperative of improving emergency department care for anxiety. This resulted in increased participation in a multi-stakeholder research partnership, critical health system support that included costs to the health system associated with implementing interventions to be tested, and submission of a patient-centered outcomes research proposal that is currently under review. In addition, Photovoice had positive benefits for participants including a therapeutic effect, may have increased group cohesion, and empowerment of patients as partners in the research process.
A case of amniotic fluid embolism successfully treated by multidisciplinary treatment
Authors: Kinishi Yuki , Ootaki Chiyo , Iritakenishi Takeshi , Fujino Yuji 
Published: November 2019

Abstract
Background
Amniotic fluid embolism (AFE) is a life-threatening obstetric emergency. Because the maternal mortality associated with AFE is very high, early recognition and prompt treatment are important for improving the prognosis. We report a case of amniotic fluid embolism successfully treated by multidisciplinary treatment.

Case presentation
A 39-year-old woman with fetal congenital heart anomaly and polyhydramnios was scheduled for induction of delivery at 37 weeks of gestation with labor epidural analgesia. Uncontrollable bleeding occurred 30 min after vaginal delivery. Based on the clinical diagnosis of AFE, massive blood transfusion, insertion of an aortic occlusion balloon catheter, and hysterectomy was performed. Total blood loss was 12,000 mL. The diagnosis of AFE was confirmed by pathological examination. She was discharged with no complications.

Conclusion
We report a case of AFE who were rescued by prompt diagnosis and treatment.
Predictive value of C-reactive protein and carotid intimal medial thickness in acute ischemic stroke
Authors: Elbelkimy Mahmoud , ELkhayat Naglaa , ElSadek Ahmed , Mansour Alia , Aboutaleb Mariam 
Published: November 2019

Abstract
Background
Elevated CRP and increased CCA-IMT are both associated with the occurrence of stroke. CRP and IMT are closely associated; the higher the CRP, the greater the carotid atherosclerosis as measured by carotid IMT.

Objectives
To study the relationship between elevated C-reactive protein as a blood biomarker and increased intimal media thickness of carotid artery, and its relation to infarct size and its impact on prognosis.

Materials and methods
This study is an analytical observational study, in which 73 patients who have recently suffered first-ever acute ischemic stroke in the anterior circulation within 72 h were recruited. Only 64 of them were able to continue the study with follow-up during the 1 month and 3 months durations. Magnetic resonance imaging for the brain was done and the infarct volume was measured. All patients had quantitative Serum CRP level within 72 h from stroke onset and carotid duplex with assessment of carotid intimal media thickness (IMT).

Results
The results showed there is a significant positive correlation between highly sensitive C-reactive protein (hs-CRP) and MRS after 1 month yet no significant correlation was found between hs-CRP and IMT.

Conclusion
Highly sensitive C-reactive protein (hs-CRP) could serve as prognostic blood biomarker in long-term follow-up of stroke patients. Non-significant correlation was found in our study between increased hs-CRP and increased intima-media thickness (CCA-IMT).
Biochar application and no-tillage practices to minimize the residues of herbicides in the seeding hole
Authors: Singha Ashutus , Rahaman Md. Arifur , Jiku Md. Abu Sayem , Faruquee Muhiuddin , Alam Md. Ashraful , Sinha Shata Rupa 
Published: November 2019

Abstract
Background
No-tillage is considered as a promising alternative for conventional farming by saving energy input and time, reducing groundwater pollution, and counteracting soil erosion and losses of soil-organic matter. Therefore, this study was carried out in north-eastern Sylhet of Bangladesh during the period of 2015–2016 to evaluate the multiple techniques of implementation in order to find a practically appropriate way to apply biochar.

Results
In this study, successfully applied of biochar and glyphosate in holes with seeds and consisted of one control (pure soil), glyphosate control, biochar control, and four glyphosate treatments with 1, 2.5, 5, and 10% biochar addition. The Gly + ch1% and Gly + ch2.5% treatments demonstrated a better emergence rate than all treatments, and at the end of the emergence, they reached more than 95%. There was no important distinction found among all the treatments in the event of shooting fresh and dry biomass. Biochar amendment treatments did not show any influence on shoot fresh biomass compared to glyphosate control and biochar 5% treatment, respectively. Gly + ch2.5% treatment showed slightly better performance than all the other treatments. The similar performance was shown in case of shoot dry weight. In case of root fresh weight, there was only a significant different observed between Gly + ch1% and Gly + ch10%. However, Gly + ch1% treatment revealed slightly higher root fresh weight compared to all the other treatments. Considering the results of the germination percentage and root morphology, it could be suggested that lower rate of biochar application showed better performance on root length and development.

Conclusions
It could be concluded that glyphosate application has mitigation effect to absorb herbicidal residues. For successful introduction of biochar application in agriculture, field acts as a huge amount of carbon sink and has also a positive effect to mitigate climate change.
Epidemiological, clinical and immune factors that influence the persistence of antiphospholipid antibodies in leprosy
Authors: Ribeiro Sandra Lúcia Euzébio , Pereira Helena Lúcia Alves , Boechat Antonio Luiz , Silva Neusa Pereira , Sato Emilia Ionue , das Graças Souza Cunha Maria , de Souza Passos Luiz Fernando , Dos-Santos Maria Cristina 
Published: November 2019

Abstract
Introduction
Antiphospholipid antibodies (aPL) are described in individuals with leprosy without the clinical features of antiphospholipid antibody syndrome (APS), a condition involving thromboembolic phenomena. We have described the persistence of these antibodies for over 5 years in patients with leprosy after specific treatment.

Objectives
To determine whether epidemiological, clinical and immunological factors played a role in the long-term persistence of aPL antibodies in leprosy patients after multidrug therapy (MDT) had finished.

Methods
The study sample consisted of 38 patients with a diagnosis of leprosy being followed up at the Dermatology and Venereology Outpatient Department at the Alfredo da Matta Foundation (FUAM) in Manaus, AM. ELISA was used to detect anticardiolipin (aCL) and anti-β2 glycoprotein I (anti-β2GPI) antibodies. Patients were reassessed on average of 5 years after specific treatment for the disease (MDT) had been completed.

Results
Persistence of aPL antibodies among the 38 leprosy patients was 84% (32/38), and all had the IgM isotype. Mean age was 48.1 ± 15.9 years, and 23 (72.0%) were male. The lepromatous form (LL) of leprosy was the most common (n = 16, 50%). Reactional episodes were observed in three patients (9.4%). Eighteen (47.37%) were still taking medication (prednisone and/or thalidomide). Mean IgM levels were 64 U/mL for aCL and 62 U/mL for anti-β2GPI. In the multivariate binary logistic regression the following variables showed a significant association: age (p = 0.045, OR = 0.91 and CI 95% 0.82–0.98), LL clinical presention (p = 0.034; OR = 0.02 and CI 95% = 0.0–0.76) and bacterial index (p = 0.044; OR = 2.74 and CI 95% = 1.03–7.33). We did not find association between prednisone or thalidomide doses and positivity for aPL (p = 0.504 and p = 0.670, respectively). No differences in the variables vascular thrombosis, pregnancy morbidity, diabetes, smoking and alcoholism were found between aPL-positive and aPL-negative patients.

Conclusion
Persistence of positivity for aPL antibodies was influenced by age, clinical presentation and bacterial index. However, further studies are needed to elucidate the reason for this persistence, the role played by aPL antibodies in the disease and the B cell lineages responsible for generation of these antibodies.
Learning progressions: framing and designing coherent sequences for STEM education
Authors: Duschl Richard A. 
Published: November 2019

AbstractThe coupled influences of scholarship in the fields of Psychology, Philosophy, and Pedagogy beginning in the 1950s, set in motion the emergence of new images, methodological perspectives, theories, and design principles about learners and learning. Advances in cognitive and sociocultural psychology, shifting images of the nature of science, recognition of the importance of disciplinary discourse practices in learning, the scaffolding of learning by tools and technologies, along with the adoption of ‘assessment for learning’ instructional strategies are among the factors that have led researchers and practitioners to advance positions that learning ought to be coordinated and sequenced along conceptual trajectories, developmental corridors, and learning progressions (LP). Following opening Introduction and LP Research Framework sections that provide an overview of the runup to LP research and development, I then turn to future research discussions and implications targeting five LP domains:Using Knowledge with Scientific Practices; Instructional Pathways – Early Childhood Learning; Teaching Experiments – Science and Mathematics; Upper/Lower Anchors for Measuring Progress; and Concepts & Practices. The Conclusion section points to overarching challenges for researchers, planners, and teachers in STEM education. There is much to learn for all!