bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-100 inhibits cell proliferation in mantle cell lymphoma by targeting mTOR
Authors: Lin Luhui , Huang Yiqun , Zhuang Wei , Lin Ping , Ma Xudong 
Published: September 2020

Abstract
Background
miR-100 is reported to be associated with cell proliferation and apoptosis. However, the function of miR-100 in mantle cell lymphoma (MCL) is unknown. The purpose of this study is to analyze the abnormal expression of miR-100 and mTOR in MCL together with their potential biological function and pathogenesis.

Method
Eighteen MCL tissue samples and 3 cell lines (Jeko-1, Mino, Granta-519) were investigated in this research study, while eighteen samples of proliferative lymphadenitis from patients and peripheral lymphocyte cells from healthy volunteers served as controls. The expression and alteration of miR-100 and mTOR mRNA were detected by RT-PCR. The expression and alteration of mTOR protein were explored by Western blot. LV-miR-100-up and LV-mTOR-RNAi were constructed and transfected by lentivirus transfection. Cell proliferation, cell apoptosis and the cell cycle were detected using CCK-8 and flow cytometry. Bioinformatics prediction software was used to predict the miR-100 target gene of mTOR. A double luciferase experiment was used to verify miR-100 targeting at the mTOR-3′-UTR. The interaction between miR-100 and mTOR was further studied using recovery experiments. GraphPad Prism 7 software (version 7.2) was used for statistical analysis, and a P value < 0.05 was considered statistically significant.

Results
We found that the expression of miR-100 mRNA in MCL tissues and cell lines was lower, while that of the mTOR protein was higher. There was a negative correlation between miR-100 and mTOR in both MCL tissues and cell lines. Promoting miR-100 and inhibiting mTOR could inhibit cell proliferation, induce cell apoptosis and block the cell cycle in the G1 phase. A double luciferase reporter assay showed that mTOR was one of the target genes of miR-100. The recovery experiment demonstrated that PV-mTOR-up partially set off the effect of LV-miR-100-up on decreasing mTOR expression, inhibiting proliferation, inducing apoptosis and blocking the cell cycle in G1 phase in both Jeko-1 and Mino cells.

Conclusions
Abnormal expression of miR-100 and mTOR was found in MCL, which included downregulation of miR-100 and upregulation of mTOR. The expression of mTOR is negatively correlated with miR-100. It may play an important role in MCL pathogenesis. miR-100 up-regulation can inhibit cell proliferation, promote cell apoptosis, and inhibit cell cycle in G1 phase by targeting the mTOR gene. miR-100 may potentially be an anti-mantle cell lymphoma gene.
Expression differences of miR-142-5p between treatment-naïve chronic myeloid leukemia patients responding and non-responding to imatinib therapy suggest a link to oncogenic ABL2, SRI, cKIT and MCL1 signaling pathways critical for development of therapy resistance
Authors: Klümper Theresa , Bruckmueller Henrike , Diewock Tobias , Kaehler Meike , Haenisch Sierk , Pott Christiane , Bruhn Oliver , Cascorbi Ingolf 
Published: September 2020

Abstract
Background
Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by constitutive activity of the tyrosine kinase BCR-ABL1. Although the introduction of tyrosine kinase inhibitors (TKIs) has substantially improved patients’ prognosis, drug resistance remains one of the major challenges in CML therapy. MicroRNAs (miRNAs), a class of short non-coding RNAs acting as post-transcriptional regulators, are implicated in CML progression and drug resistance. The aim of the present study was to analyze the miRNA expression profiles of 45 treatment-naïve CML patients in chronic phase (28 peripheral blood and 17 bone marrow samples) with respect to future response to imatinib therapy.

Methods
TaqMan low density arrays were used to analyze the miRNA expression pattern of the patient samples. For selected microRNAs, reporter gene assays were performed to study their ability to regulate CML associated target genes.

Results
Significant lower expression levels of miR-142-5p were identified in both, peripheral blood and bone marrow samples of future non-responders suggesting a potential tumor suppressor role of this miRNA. This was supported by reporter gene assays that identified the survival, proliferation and invasion promoting CML related genes ABL2, cKIT, MCL1 and SRI as targets of miR-142-5p and miR-365a-3p, the latter identified as potential biomarker in peripheral blood samples.

Conclusion
MiR-142-5p and to a certain extend also miR-365a-3p were able to discriminate treatment-naïve CML patients not responding to imatinib in the course of their treatment from patients, who responded to therapy. However, further large-scale studies should clarify if the identified miRNAs have the potential as predictive biomarkers for TKI resistance.
Early school entrance and middle-run academic performance in Mexico: evidence for 15-year-old students from the PISA test
Authors: Aguayo-Téllez Ernesto , Martínez-Rodríguez Flor E. 
Published: September 2020

AbstractUsing an unanticipated policy reform in Mexico that shifted 4 months the cutoff date for elementary school eligibility in 2006 and information on academic performance of 15-year-old students from the Programme for International Student Assessment (PISA) 2018 survey, who entered first grade in 2006, this paper estimates the effects of starting first grade without having reached 6 years of age on middle-run academic performance. We found that entering school at a younger age increases the probability of having repeated at least one academic year by 7 percentage points and reduces academic performance (measured throughout PISA’s score points) between 6 and 10 score points in mathematics, reading and science (a Cohen’s d effect size between 0.06 and 0.10). We did not find evidence to claim that the effect of early entrance on academic performance is different for girls and boys or for urban and rural locations.
Acute kidney injury after aneurysmal subarachnoid hemorrhage: is chloride really responsible?
Authors: Gueret Gildas , Lefebvre Pierre , Le Maguet Pascale , Fabre Renaud 
Published: September 2020

AbstractSadan et al. find an association between acute kidney injury and high chloride containing a hypertonic solution. Recent large prospective non-randomized studies bring conflicting results on the relationship between chloride and acute kidney injury. We discuss Sadan et al.’s results according to the recent literature.
The authors’ response
Authors: Sadan Ofer , Samuels Owen 
Published: September 2020

AbstractIn response to comments raised, we acknowledge the shortcomings of our study. It is a small study. However, it is a pilot study, which is not meant to create generalizable data, rather to explore new potential directions. To this end, our conclusions were clearly supported by the results. We demonstrated that administration of 16.4% NaCl/Na-acetate solution was feasible, safe, and was associated with lower rates of AKI. We share the call that large RCTs are required to follow this pilot study and hope that our data will stimulate the ongoing discussion regarding the role of chloride in AKI mechanism.
Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity
Authors: Czapela Fabiane Fernanda , Kubeneck Simone , Preczeski Karina Paula , Dalastra Caroline , Scapini Thamarys , Bonatto Charline , Stefanski Fábio Spitza , Camargo Aline Frumi , Zanivan Jessica , Mossi Altemir José , Fongaro Gislaine , Treichel Helen 
Published: September 2020

AbstractPretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes. In the present work, an ultrasonic probe reaction system was used to evaluate the potential of swine hair pretreatment. The pretreated material was submerged with non-pretreated residues for 9 days to obtain the enzyme. Enzyme activity was measured in the extracts obtained using the ultrasonic probe, ultrasonic bath, and microwave. We also used the enzymatic concentration technique with NaCl and acetone. Homemade enzymatic extracts were evaluated for their ability to degrade swine hair and chicken feathers by comparing them with the activities commercial enzymes. Macrobeads gave greater energy dissipation in less time, providing greater enzyme activity (50.8 U/mL over 3 days). In terms of waste degradation, non-pretreated swine hair was more promising. The ultrasonic probe reaction system had the potential to evaluate increased enzyme activity (38.4% relative activity) and the enzyme concentration increased activity by 53.5%. The homemade enzymatic extract showed promise for degradation of keratinous residues.
Fibre attributes and mapping the cultivar influence of different industrial cellulosic crops (cotton, hemp, flax, and canola) on textile properties
Authors: Shuvo Ikra Iftekhar 
Published: September 2020

AbstractNatural lignocellulosic fibres (NLF) extracted from different industrial crops (like cotton, hemp, flax, and canola) have taken a growing share of the overall global use of natural fibres required for manufacturing consumer apparels and textile substrate. The attributes of these constituent NLF determine the end product (textiles) performance and function. Structural and microscopic studies have highlighted the key behaviors of these NLF and understanding these behaviors is essential to regulate their industrial production, engineering applications, and harness their benefits. Breakthrough scientific successes have demonstrated textile fibre properties and significantly different mechanical and structural behavioral patterns related to different cultivars of NLF, but a broader agenda is needed to study these behaviors. Influence of key fibre attributes of NLF and properties of different cultivars on the performance of textiles are defined in this review. A likelihood analysis using scattergram and Pearson’s correlation followed by a two-dimensional principal component analysis (PCA) to single-out key properties explain the variations and investigate the probabilities of any cluster of similar fibre profiles. Finally, a Weibull distribution determined probabilistic breaking tenacities of different fibres after statistical analysis of more than 60 (N > 60) cultivars of cotton, canola, flax, and hemp fibres.
Implementing nudges for suicide prevention in real-world environments: project INSPIRE study protocol
Authors: Davis Molly , Wolk Courtney Benjamin , Jager-Hyman Shari , Beidas Rinad S. , Young Jami F. , Mautone Jennifer A. , Buttenheim Alison M. , Mandell David S. , Volpp Kevin G. , Wislocki Katherine , Futterer Anne , Marx Darby , Dieckmeyer E. L. , Becker-Haimes Emily M. 
Published: September 2020

Abstract
Background
Suicide is a global health issue. There are a number of evidence-based practices for suicide screening, assessment, and intervention that are not routinely deployed in usual care settings. The goal of this study is to develop and test implementation strategies to facilitate evidence-based suicide screening, assessment, and intervention in two settings where individuals at risk for suicide are especially likely to present: primary care and specialty mental health care. We will leverage methods from behavioral economics, which involves understanding the many factors that influence human decision making, to inform strategy development.

Methods
We will identify key mechanisms that limit implementation of evidence-based suicide screening, assessment, and intervention practices in primary care and specialty mental health through contextual inquiry involving behavioral health and primary care clinicians. Second, we will use contextual inquiry results to systematically design a menu of behavioral economics-informed implementation strategies that cut across settings, in collaboration with an advisory board composed of key stakeholders (i.e., behavioral economists, clinicians, implementation scientists, and suicide prevention experts). Finally, we will conduct rapid-cycle trials to test and refine the menu of implementation strategies. Primary outcomes include clinician-reported feasibility and acceptability of the implementation strategies.

Discussion
Findings will elucidate ways to address common and unique barriers to evidence-based suicide screening, assessment, and intervention practices in primary care and specialty mental health care. Results will yield refined, pragmatically tested strategies that can inform larger confirmatory trials to combat the growing public health crisis of suicide.
Wages, integration, migration motivation: cases of export industry employees in Tijuana and Tangiers-Tetouan
Authors: Kopinak Kathryn , Hennebry Jenna , Soriano-Miras Rosa Maria , Trinidad Requena Antonio 
Published: September 2020

AbstractRecent commentaries on migration integration suggest that researchers focus more on cities than nation states and include considerations of political economy, societal inequality and shifts in production. This article analyses how different aspects of wages of export industry (EI) migrant and borderlander workers in Tijuana, Mexico and Tangiers-Tetouan, Morocco limit their socioeconomic integration and lead to their greater identification with foreign standards, stimulating emigration northward. Using the new theory of migration systems, real and indirect wages are found to be major system elements initiating and deepening inequality, and providing for comparisons between natives, foreigners and expatriates, creating relative deprivation. System dynamics, such as border characteristics, insecurity, and currency markets uniquely contribute to inadequate settlement south of the US border. While EI wages are shown to poorly integrate migrant workers structurally in both cities, employment there supports incorporation into northern countries. The paper contributes to migration integration research by adding to the few studies which use wages as an indicator of integration and by focusing on low income regions instead of high income countries which constitute most previous research.
S100A14 serum level and its correlation with prognostic factors in breast cancer
Authors: Al-Ashkar Noor , Zetoune Almoutassem Billah 
Published: September 2020

Abstract
Background
Breast cancer is the most commonly occurring cancer in women worldwide. S100A14 is a novel important member of S100 proteins family. Its importance is due to its role in tumorigenesis and metastasis process. In this study, we aimed to determine serum levels of S100A14 protein in breast cancer patients and healthy individuals to know if it can be suggested as a new biomarker for breast cancer and to reveal whether it is correlated with cancer pathological features.

Methods
This cross-sectional study was performed in two groups: study group contains 46 breast cancer patients (29 metastatic and 17 non-metastatic) and control group contains 22 healthy women. Enzyme-linked immunoabsorbent assay was performed to determine S100A14 protein levels in samples. Pathological data were obtained for each patient. The data were statistically analyzed using Kruskal-Wallis H, Mann-Whitney U, and Spearman correlation tests.

Results
S100A14 serum levels were elevated in study group compared with control group (P < 0.05). S100A14 serum levels were significantly increased in distant breast cancer patients compared with regional breast cancer patients (P = 0.001). There was a strong positive correlation between serum S100A14 level and tumor grade (rs = 0.713, P < 0.001).

Conclusion
Our study indicated that S100A14 serum levels are elevated in breast cancer patients compared with control individuals. High S100A14 serum levels were correlated with poor tumor differentiation so it might have a prognostic significance for breast cancer tumors. The elevation of S100A14 levels in distant breast cancer patients suggests the ability of using serum S100A14 as a biomarker for detection of breast cancer metastasis.
Multiple Endocrine Neoplasia Type 1 (MEN1) Phenocopy Due to a Cell Cycle Division 73 (CDC73) Variant
Authors: Lines Kate E , Nachtigall Lisa B , Dichtel Laura E , Cranston Treena , Boon Hannah , Zhang Xun , Kooblall Kreepa G , Stevenson Mark , Thakker Rajesh V 
Published: September 2020

Abstract
Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder characterized by the combined occurrence of parathyroid tumors, pituitary adenomas, and pancreatic neuroendocrine neoplasms (PNENs). MEN1 is caused by germline MEN1 mutations in &gt; 75% of patients, and the remaining 25% of patients may have mutations in unidentified genes or represent phenocopies with mutations in genes such as cell cycle division 73 (CDC73), the calcium sensing receptor (CASR), and cyclin-dependent kinase inhibitor 1B (CDKN1B), which are associated with the hyperparathyroidism-jaw tumor syndrome, familial hypocalciuric hypercalcemia type 1, and MEN4, respectively. Here, we report a heterozygous c.1138C&gt;T (p.Leu380Phe) CDC73 germline variant in a clinically diagnosed MEN1 patient, based on combined occurrence of primary hyperparathyroidism, acromegaly, and a PNEN. Characterization of the PNEN confirmed it was a neuroendocrine neoplasm as it immuno-stained positively for chromogranin and glucagon. The rare variant p.Leu380Phe occurred in a highly conserved residue, and further analysis using RNA-Scope indicated that it was associated with a significant reduction in CDC73 expression in the PNEN. Previously, CDC73 mutations have been reported to be associated with tumors of the parathyroids, kidneys, uterus, and exocrine pancreas. Thus, our report of a patient with PNEN and somatotrophinoma who had a CDC73 variant, provides further evidence that CDC73 variants may result in a MEN1 phenocopy.
Experience in application of modified sling in treatment of Moderate Stress Urinary Incontinence
Authors: Guo Jing-yang , An Feng , Gu De-qiang , Yang Wenzeng 
Published: September 2020

Objective: To observe the clinical significance of modified sling in the treatment of moderate stress urinary incontinence (SUI).
Methods: From January 2016 to January 2019, eighty patients with moderate urinary incontinence who were hospitalized in our hospital were randomly divided into two groups. 40 patients in the experimental group underwent modified sling transvaginal tension-free mid-urethral suspension. Modification method of the sling: cut the sling to a remaining length of about 6~7cm, properly connect the barbed sutures (V-LOCK) on both sides of the sling, and insert the urinary incontinence sling from the urethra to the obturator membrane, from the obturator membrane to the thigh. The inner skin area is replaced by the V-LOCK line. The 40 patients in the control group were unmodified ordinary slings. The operation time, the local pain of the inner thigh after the operation, and the improvement of postoperative urinary incontinence symptoms were compared and analyzed between the two groups.
Results: Both groups of patients were successfully operated. The operation time was 16.36 minutes in the experimental group and 27.18 minutes in the control group. The difference in operation time between the two groups was statistically significant (p=0.00); the catheter was pulled out on the third day after the operation. One patient in the group had urinary effort, four patients still had urinary incontinence symptoms, the remaining 35 patients had good urinary control (effective rate 87.5%), five patients in the control group still had urinary incontinence, two patients had urinary effort, and the remaining 33 patients had urinary control Good, (effective rate 87.5%), there was no significant difference in surgical effectiveness between the two groups (p=0.53); follow-up for 12 to 36 months, no significant long-term complications occurred, the pain score of the inner thigh of the experimental group was significantly lower than that of the control group ,statistically significant (p=0.04).
Conclusion: The efficacy of the modified sling in the treatment of moderate SUI is the same as that of the traditional sling, but the operation time is shorter, the operation is simpler, and the local pain is significantly reduced.
doi: https://doi.org/10.12669/pjms.36.7.2619
How to cite this:Guo J, An F, Gu D, Yang W. Experience in application of modified sling in treatment of Moderate Stress Urinary Incontinence. Pak J Med Sci. 2020;36(7):1664-1670. doi: https://doi.org/10.12669/pjms.36.7.2619
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Prevalence of anorexia nervosa and bulimia nervosa among women who recently attempted suicide compared with suicide non-attempting women
Authors: Rostami Hamzeh 
Published: September 2020

Aim of the studyThe suicide risk in patients with eating disorders is 5-6 times higher than that of the general population. Due to the high likelihood of re-suicide in suicide survivors, in this study, we investigated the prevalence of the anorexia nervosa and bulimia nervosa among women who recently attempted suicide compared with suicide non-attempting women.Subject or material and methodsIn this case-control study, two groups of 30 women aged 15-45-year-old were investigated after a suicide attempt. Case group was admitted to the Razi Hospital poisoning ward and the control group was admitted to other wards of the hospital. Data were collected using the EAT-26 questionnaire and a clinical interview based on the SCID-V. Finally, the results were analyzed using the SPSS version 21.ResultsThe both groups’ mean age was 26.96± 6.3 and 27.2±7.16 years, respectively. There was no significant difference in age, employment status, marital status, and education level between the two groups (P>0.05). The prevalence of both disorders did not show a significant interaction with demographic characteristics. The average prevalence of both disorders among the case group was 32.78±8.1 and 37.42±7.21, respectively, and was significantly different in women who had recently attempted suicide compared to their counterparts (p <0.05).DiscussionOur findings are consistent with all previously conducted studies.ConclusionsThe prevalence of these disorders was significantly higher in women who had recently committed suicide compared to the control group (p <0.05), although there was no significant difference in the prevalence of both disorders with other sociodemographic factors.