bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular and immunological characterization of grass carp (Ctenopharyngodon idella) parvalbumin Cten i 1: A major fish allergen in Hong Kong
Authors: Leung Nicki Yat Hin , Leung Agnes Sze Yin , Xu Kary Jia Ying , Wai Christine Yee Yan , Lam Cheuk Yin , Wong Gary Wing Kin , Leung Ting Fan 
Published: April 2020

AbstractBackgroundGrass carp is the most commonly consumed fish species in Hong Kong. The allergenicity of grass carp and its allergen content are yet to be reported. This study characterized the major allergen in grass carp and investigated its allergenicity.MethodsSixty‐nine subjects with history of IgE‐mediated allergic reaction to grass carp were recruited. The protein content in steamed grass carp extract was resolved by SDS‐PAGE, and the major allergen was identified by immunoblotting with serum from subjects allergic to grass carp. The identity of allergen was elucidated by mass spectrometry and amino acid sequence obtained by amplifying the specific gene from cDNA library of grass carp. The cross‐reactivity between parvalbumins from grass carp and other phylogenetically close (common carp) or commercially important (cod and salmon) species was investigated by competitive inhibition ELISA.ResultsA major IgE‐binding protein was found at approximately 9 kDa and identified as parvalbumin by immunoblotting and mass spectrometry. Grass carp parvalbumin was more allergenic than common carp, salmon, and cod parvalbumins despite sharing high sequence homology. This newly identified major allergenic parvalbumin isoform from grass carp was registered as Cten i 1 in the World Health Organization and International Union of Immunological Societies allergen database.ConclusionsGrass carp parvalbumin is identified as the major fish allergen in Hong Kong. The strong allergenicity of Cten i 1 contributes to the high IgE reactivity of grass carp. Grass carp, among other fish species, should be considered when managing fish‐allergic patients.
Molecular underpinnings of methyl jasmonate‐induced resistance in Norway spruce
Authors: Mageroy Melissa H. , Wilkinson Samuel W. , Tengs Torstein , Cross Hugh , Almvik Marit , Pétriacq Pierre , Vivian‐Smith Adam , Zhao Tao , Fossdal Carl Gunnar , Krokene Paal 
Published: April 2020

AbstractIn response to various stimuli, plants acquire resistance against pests and/or pathogens. Such acquired or induced resistance allows plants to rapidly adapt to their environment. Spraying the bark of mature Norway spruce (Picea abies) trees with the phytohormone methyl jasmonate (MeJA) enhances resistance to tree‐killing bark beetles and their associated phytopathogenic fungi. Analysis of spruce chemical defenses and beetle colonization success suggests that MeJA treatment both directly induces immune responses and primes inducible defenses for a faster and stronger response to subsequent beetle attack. We used metabolite and transcriptome profiling to explore the mechanisms underlying MeJA‐induced resistance in Norway spruce. We demonstrated that MeJA treatment caused substantial changes in the bark transcriptional response to a triggering stress (mechanical wounding). Profiling of mRNA expression showed a suite of spruce inducible defenses are primed following MeJA treatment. Although monoterpenes and diterpene resin acids increased more rapidly after wounding in MeJA‐treated than control bark, expression of their biosynthesis genes did not. We suggest that priming of inducible defenses is part of a complex mixture of defense responses that underpins the increased resistance against bark beetle colonization observed in Norway spruce. This study provides the most detailed insights yet into the mechanisms underlying induced resistance in a long‐lived gymnosperm.
Primary cutaneous marginal zone lymphoma treated with doxycycline in a pediatric patient
Authors: Jothishankar Balaji , Di Raimondo Cosimo , Mueller Lisa , Zain Jasmine , Parekh Vishwas , Abdulla Farah 
Published: April 2020

AbstractWe present the third reported case of a primary cutaneous marginal zone lymphoma (PCMZL) treated with doxycycline in a pediatric patient with negative serology for Borrelia burgdorferi. A 14‐year‐old boy presented with multiple asymptomatic erythematous papules and nodules on his extremities and trunk which biopsy confirmed to be PCMZL. He was started on doxycycline and experienced a near‐complete response. Given the favorable side effect profile of doxycycline and the indolent nature of PCMZL, we believe doxycycline is a possible therapy for PCMZL pediatric patients who have widely disseminated cutaneous disease.
Percutaneous removal of biliary stones post‐liver transplant in a pediatric patient: Case report and review of the literature
Authors: Rohringer Taryn J. , Hannick Jessica H. , Lorenzo Armando , Avitzur Yaron , Temple Michael , Parra Dimitri A. 
Published: April 2020

AbstractThis case report describes an 8‐year‐old girl who underwent a segmental LT for a primary diagnosis of citrullinemia at the age of 12 months. She presented with cholangitis secondary to stenosis of the biliary‐enteric anastomosis. MRI revealed dilatation of intrahepatic bile ducts associated with multiple stones. An endoscopic approach failed to decompress the bile ducts and remove the stones. A percutaneous approach was then undertaken. After placement of a temporary external biliary drain for 12 days, a 26 French sheath was placed to access the bile ducts. Using a 14Fr flexible cystoscope, 80%‐90% of the biliary stones were removed. This was followed by antegrade balloon dilatation of the biliary‐enteric anastomosis. Two months later, the procedure was repeated, resulting in complete clearance of the biliary stones. An internal‐external biliary drain was maintained in placed for 10 months. The patient has been asymptomatic, with no evidence of stone recurrence for 13 months after drain removal. Percutaneous biliary stone removal is commonly performed in adults with non‐transplanted livers, especially in complex cases, and has also been shown to be successful in the pediatric population. However, it is rarely reported in transplanted livers in adults, and to the best of our knowledge, no pediatric cases have been reported. This case illustrates that this technique can be successfully utilized in pediatric LT patients.
Neural correlations of the influence of self‐relevance on moral decision‐making involving a trade‐off between harm and reward
Authors: Zhan Youlong , Xiao Xiao , Tan Qianbao , Li Jin , Fan Wei , Chen Jie , Zhong Yiping 
Published: April 2020

AbstractAlthough economists have suggested that humans generally prioritize maximizing their own self‐interest rather than others’ when distributing rewards, recent psychological studies have shown that people are hyperaltruistic when allocating physical harm to themselves and others during moral decision‐making. However, little is known about how the neurocognitive mechanisms underlying self‐relevance modulate this behavioral tendency under different degrees of physical harm. This study adopted a moral decision‐making task to investigate behavioral and neural processes during moral decision‐making involving different levels of self‐relevance and physical harm. Event‐related potentials were measured while participants made trade‐offs of different monetary gains for themselves against painful electric shocks experienced by the receivers (self, friend, or stranger). These results suggest that early anterior N1, indexing fast and automatic moral intuitional process, decreased during the strong conflict trade‐off decisions involving strong painful electric shocks and much monetary gains. Lower self‐relevance enhanced the aversive experience and increased the mental cost of resolving moral conflict, reflected by a larger P260‐LPP (300–450 ms) effect during weaker conflict decisions toward strangers than themselves and friends. However, this effect was weaker during strong conflict decisions. When making decisions about whether to shock others to gain money for themselves, participants were hyperaltruistic, foregoing greater self‐interest to restrain harm directed toward strangers than themselves or friends. These findings shed light on the neural basis of the tension between egoistic and altruistic tendencies during moral decision‐making integrating benefits and harms.
Quercetin supports bovine preimplantation embryo development under oxidative stress condition via activation of the Nrf2 signalling pathway
Authors: Khadrawy Omar , Gebremedhn Samuel , Salilew‐Wondim Dessie , Rings Franca , Neuhoff Christiane , Hoelker Michael , Schellander Karl , Tesfaye Dawit 
Published: April 2020

AbstractNrf2 is a master regulator for antioxidant machinery against oxidative stress in bovine preimplantation embryos. The endogenous or exogenous modulation of Nrf2‐KEAP1 system in bovine embryos may contribute to the understanding of the mechanisms behind the response of embryos to stress conditions. Therefore, here we aimed to investigate the protective effect of quercetin on bovine preimplantation embryos exposed to higher atmospheric oxygen concentration. For that, blastocysts, which were developed from zygotes cultured in media supplemented with or without quercetin under high oxygen level (20%), were subjected intracellular ROS level and mitochondrial analysis, and determining blastocyst formation rate and total cell number. Moreover, mRNA and protein expression level of Nrf2 and selected downstream antioxidant genes were investigated in the resulting blastocysts. Quercetin supplementation in vitro culture did not affect cleavage and blastocyst rate until day 7. However, quercetin supplementation resulted in higher blastocyst total cell number and reduction of intracellular ROS level accompanied by increasing mitochondrial activity compared with control group in both day 7 and day 8 blastocysts. Moreover, quercetin supplementation induced mRNA and protein of Nrf2 with subsequent increase in the expression of downstream antioxidants namely: NQO1, PRDX1, CAT and SOD1 antioxidants. In conclusion, quercetin protects preimplantation embryos against oxidative stress and improves embryo viability through modulation of the Nrf2 signalling pathway.
'Positive Christianity': Theological rationales and legacies
Authors: Buesnel Ryan 
Published: April 2020

AbstractIn response to the 1933 election of Adolf Hitler as German Chancellor, the various German Christian movements set about the task of constructing a national Reich Church which would complement Nazi policy – an effort that required removing the legacy of Judaism from both dogma and ecclesial tradition. To achieve this, the German Christians were able to draw on an established legacy of 'Positive Christianity' in order to offer a theological rationale for Nazi ideology. This paper traces the development of Positive Christianity and examines key themes as they are expressed in two of its representative works: Ludwig Müller's What is Positive Christianity? and Cajus Fabricius's Positive Christianity in the New State. The paper will close an analysis of contemporary debates relating to Positive Christianity's influence on Nazism and the Church.
Conversation analysis, cyberpsychology and online interaction
Authors: Meredith Joanne 
Published: April 2020

AbstractIn this paper, I explore how conversation analysis (CA) can be used as a method for analysing online interaction. As the number and quantity of online communication platforms have proliferated, there has been a growing interest in social psychology about the impact and effectiveness of online, text‐based communication. A number of theories have been used and developed to explain how online communication might impact upon relationships and effective communication. However, this paper argues that in order to explore the differences between online and offline interaction an analysis of online behaviour is needed. CA allows for an in‐depth, sequential and discursive analysis of real‐life online interactions. It can explore the ways in which the affordances of the interactional platforms are oriented to or made relevant in the interaction. The utility of CA is demonstrated through a number of examples, highlighting how this method can be used to broaden our understanding of how online communication works in practise.
The histone methylation readers MRG1/MRG2 and the histone chaperones NRP1/NRP2 associate in fine‐tuning Arabidopsis flowering time
Authors: An Zengxuan , Yin Liufan , Liu Yuhao , Peng Maolin , Shen Wen‐Hui , Dong Aiwu 
Published: April 2020

SummaryHistones are highly basic proteins involved in packaging DNA into chromatin, and histone modifications are fundamental in epigenetic regulation in eukaryotes. Among the numerous chromatin modifiers identified in Arabidopsis (Arabidopsis thaliana), MORF‐RELATED GENE (MRG)1 and MRG2 have redundant functions in reading histone H3 lysine 36 trimethylation (H3K36me3). Here, we show that MRG2 binds histone chaperones belonging to the NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1) family, including NAP1‐RELATED PROTEIN (NRP)1 and NRP2. Characterization of the loss‐of‐function mutants mrg1 mrg2, nrp1 nrp2 and mrg1 mrg2 nrp1 nrp2 revealed that MRG1/MRG2 and NRP1/NRP2 regulate flowering time through fine‐tuning transcription of floral genes by distinct molecular mechanisms. In particular, the physical interaction between NRP1/NRP2 and MRG1/MRG2 inhibited the binding of MRG1/MRG2 to the transcription factor CONSTANS (CO), leading to a transcriptional repression of FLOWERING LOCUS T (FT) through impeded H4K5 acetylation (H4K5ac) within the FT chromatin. By contrast, NRP1/NRP2 and MRG1/MRG2 act together, likely in a multiprotein complex manner, in promoting the transcription of FLOWERING LOCUS C (FLC) via an increase of both H4K5ac and H3K9ac in the FLC chromatin. Because the expression pattern of FLC represents the major category of differentially expressed genes identified by genome‐wide RNA‐sequencing analysis in the mrg1 mrg2, nrp1 nrp2 and mrg1 mrg2 nrp1 nrp2 mutants, it is reasonable to speculate that the NRP1/NRP2‐MRG1/MRG2 complex may be involved in transcriptional activation of genes beyond FLC and flowering time control.
Investigation of an Energy Source Temperature for NH3 + NaSCN and NH3 + LiNO3 Absorption Refrigeration Systems
Authors: Modi Nishant , Pandya Bhargav , Patel Jatin 
Published: April 2020

Abstract
This paper evaluates the energy source temperature for novel salts based ammonia/sodium thiocyanate (NH3 + NaSCN) and ammonia/lithium nitrate (NH3 + LiNO3) absorption refrigeration systems. Minimum energy source temperature (cutoff) required to initiate the cooling, critical energy source temperature for optimized thermodynamic performance and possible maximum energy source temperature to avoid crystallization have been determined, and empirical correlations are developed to facilitate continuous operation of the system. A comparison of cutoff energy source temperature depicts that the NH3 + NaSCN pair requires averagely 6 –7 °C higher cutoff temperature compared with the NH3 + LiNO3 pair. Contradictory to this, the maximum coefficient of performance (COP) of the NH3 + NaSCN pair is 7.02% higher than that the NH3 + LiNO3 pair. However, NH3 + NaSCN pair operates in a very narrow range of energy source temperature. From the P − T − X diagram, the crystallization phenomenon is clarified and the maximum energy source temperature has been determined beyond which the system would not function due to crystallization problems. For −10 °C evaporator temperature, the energy source temperature should be controlled between 87 °C and 115 °C for the NH3 + NaSCN pair and between 80 °C and 147 °C for the NH3 + LiNO3 pair.
Experimental Study of a Bulb Turbine Model During Start-Up and at Speed-No-Load Conditions, Based on the Measurement of Unsteady Pressure
Authors: Coulaud Maxime , Lemay Jean , Deschenes Claire 
Published: April 2020

Abstract
Experimental analysis of a bulb turbine during the start-up sequence and in speed-no-load (SNL) operating conditions was performed in a closed-loop circuit. This study focuses on pressure fluctuations across the machine. The turbine was equipped with 26 pressure sensors on one runner blade and 16 in the stationary reference frame. Strain measurements were also performed on two other runner blades. The first section of this analysis focuses on SNL operating conditions using standard Fourier data processing. The results show that three rotating flow phenomena are only present close to the runner. One of them corresponds to the interblade vortex at f/fr=4.00, whereas the two others, which have subsynchronous runner frequencies, are consistent with a possible rotating stall. These phenomena, which exist predominantly on the suction side, have a strong influence on runner blade strain. The second section of the study concentrates on a time-frequency analysis using the Morlet wavelet transform. It reveals that the two subsynchronous flow structures appear at the end of the start-up and exhibit bistable behavior. As well, each of these phenomena acts differently on the blade. These phenomena also interact with the interblade vortex.
A Quantitative Structure Tribo-Ability Relationship Model for Predicting the Antiwear Properties of Ionic Liquids as Lubricant Additives in Dimethyl Sulfoxide
Authors: Zhao Jiang , Gao Xinlei , Chen Tao , Wang Tingting , Li Yang 
Published: April 2020

Abstract
The structural parameters of 23 ionic liquids were calculated by the Hartree–Fock ab initio method. The relationship between the antiwear properties of ionic liquids dissolved in dimethyl sulfoxide and the structural parameters of the ionic liquids was studied using multiple linear regression analysis. A quantitative structure tribo-ability relationship model with good fitting and prediction ability was established. The results show that entropy is the most important structural parameter affecting the antiwear performance of ionic liquid-dimethyl sulfoxide systems. The entropy of the ionic liquid is related to the hydrogen bonding in the system, and an orderly arrangement of this hydrogen bonding is beneficial in terms of improving antiwear performance.
Comparison of the Y-Balance Test and Star Excursion Balance Test: Utilization of a Discrete Event Simulation
Authors: Gabriel Emily H. , Powden Cameron J. , Hoch Matthew C. 
Published: April 2020

Context: The Y-Balance Test (YBT) and Star Excursion Balance Test (SEBT) are commonly used to detect deficits in dynamic postural control. There is a lack of literature on the differences in reach distances and efficiency of the tests. Objective: To compare the reach distances of the YBT and SEBT. An additional aim was to compare the time necessary to administer the 2 tests and utilize a discrete event simulation to determine the number of participants who could be screened within different scenarios. Design: Cross-sectional. Laboratory Patients: Twenty-four physically active individuals between the ages of 18–35 years volunteered to participate in this study (M/F: 11/13; age 22.78 [2.63] y, height 68.22 [4.32] cm, mass 173.27 [10.96] kg). Intervention: The participants reported to the laboratory on one occasion and performed the YBT and SEBT. The anterior, posteromedial, and posterolateral reach distances were recorded for each test. In addition, the time to administer each test was recorded in seconds. Main Outcome Measures: The average reach distances and time for each test were used for analysis. Paired t tests were utilized to compare the reach distances and time to administer the 2 tests. A discrete event simulation was used to determine how many participants could be screened using each test. Results: The anterior reach for the SEBT (64.52% [6.07%]) was significantly greater than the YBT (61.66% [6.37%]; P < .01). The administration time for the YBT (512.42 [123.97] s) was significantly longer than the administration time for the SEBT (364.96 [69.46] s; P < .01). The discrete event simulation revealed more participants could be screened using the SEBT when compared with the YBT for every situation. Conclusion: Scores on the anterior reach of the SEBT are larger when compared with the YBT. The discrete event simulation can successfully be used to determine how many participants could be screened with a certain amount of resources given the use of a specific test.
Three-dimensional strain accumulation and partitioning in an arcuate orogenic wedge: An example from the Himalaya
Authors: Fan Suoya , Murphy Michael A. 
Published: April 2020

AbstractIn this study, we use published geologic maps and cross-sections to construct a three-dimensional geologic model of major shear zones that make up the Himalayan orogenic wedge. The model incorporates microseismicity, megathrust coupling, and various derivatives of the topography to address several questions regarding observed crustal strain patterns and how they are expressed in the landscape. These questions include: (1) How does vertical thickening vary along strike of the orogen? (2) What is the role of oblique convergence in contributing to along-strike thickness variations and the style of deformation? (3) How do variations in the coupling along the megathrust affect the overlying structural style? (4) Do lateral ramps exist along the megathrust? (5) What structural styles underlie and are possibly responsible for the generation of high-elevation, low-relief landscapes? Our model shows that the orogenic core of the western and central Himalaya displays significant along-strike variation in its thickness, from ∼25–26 km in the western Himalaya to ∼34–42 km in the central Himalaya. The thickness of the orogenic core changes abruptly across the western bounding shear zone of the Gurla Mandhata metamorphic core complex, demonstrating a change in the style of strain there. Pressure-temperature-time results indicate that the thickness of the orogenic core at 37 Ma is 17 km. Assuming this is constant along strike from 81°E to 85°E indicates that, the western and central Nepal Himalaya have been thickened by 0.5 and 1–1.5 times, respectively. West of Gurla Mandhata the orogenic core is significantly thinner and underlies a large 11,000 km2 Neogene basin (Zhada). A broad, thick orogenic core associated with thrust duplexing is collocated with an 8500 km2 high-elevation, low-relief surface in the Mugu-Dolpa region of west Nepal. We propose that these results can be explained by oblique convergence along a megathrust with an along-strike and down-dip heterogeneous coupling pattern influenced by frontal and oblique ramps along the megathrust.