bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Application of Digital Leadership of Subdistrict Head on Public Service Performance with the Delegation of Regent/Mayor Authorities as the Moderating Variable
Authors: Mardiana Dwi 
Published: November 2020

The low Human Development Index (HDI) for the protected areas is a government-focused development priority, especially in the health sector. Attempts should be made to enhance the efficiency of regional public services related to efforts to break the chain of Covid-19. Subdistrict heads have also contributed greatly to the optimization of task assignments to the apparatus. An appeal to implement social distancing becomes an obstacle for the subdistrict head for there is no face-to-face meeting with the assigned apparatus. The subdistrict heads are required to be able to adapt to the situation. Thus, the subdistrict heads must use digital media in carrying out their duties. The leadership style that influences service performance and is appropriate to be implemented in this phenomenon is digital leadership. Meanwhile, the delegation of authority is a variable that might strengthen the influence of leadership on the performance of public services. This study aims to investigate the effect of the subdistrict head’s digital leadership on the performance of public service employees in the subdistricts of Pendalungan with the delegation of regent/mayor authority as a moderating variable.
Comparison of Tenofovir with Telbivudine in Preventing Hepatitis B Transmission in Mothers with High Viral Load: A Retrospective Cohort Study
Authors: Wang Ming , Zhu Yunxia , Pang Qiumei , Li Ran , Zhang Hua 
Published: November 2020

Abstract
Background: Little data exist regarding the comparison of efficacy and safety between tenofovir disoproxil fumarate (TDF) and Telbivudine (LdT) in late pregnancy on preventing hepatitis B mother-to-child transmission (MTCT) in real-world settings. Methods: We retrospectively included HB-s antigen (HBsAg) positive mothers with HBV DNA ≥2*105IU/mL to receive TDF or LdT after gestational weeks 24～32 weeks. All infants received standard immunoprophylaxis. Primary outcomes were MTCT rates at infants’ age of 52 weeks and safety of TDF or LdT use. Secondary outcomes were the decline of HBV-DNA levels at delivery and rates of on-treatment and off-treatment alanine aminotransferase (ALT) elevation>2 upper limits of normal (ULN) during the study.Results: Of 1407 women, 209 received TDF and 1198 received LdT treatment. There were no differences between mean duration of TDF and LdT treatment (TDF vs. LdT: 11.76±2.20 weeks vs 11.64±2.79 weeks, p>0.05). At birth, 213 (9.8%) infants in the TDF-group were HBsAg positive, lower than 1180 (20.8%) in the LdT-group (p<0.001). Among 1405 infants (TDF/LdT=213/1192) of the 1385 (TDF/LdT=205/1180) women completed the 52-weeks study, intention‐to‐treat analysis indicated 1 (0.5 %) (1 infant was lost to follow-up) in TDF treated mothers and 3(0.3 %) in LDT treated mothers (3 infants were lost to follow-up). There was no difference between TDF group and LdT (p=0.483). On-treatment analysis indicated 0% HBsAg positive infants in the two groups (p=1.0). Levels of HBV-DNA decline in TDF-treated mothers were observed comparable to LdT-treated mothers (4.05±0.93 log10IU/mlvs.3.99±1.30 log10IU/ml, p=0.499). TDF-treated mothers had complained more symptoms of the digestive system and less arthralgia than LdT-treated mothers. All adverse events of two groups were grade I-II. Alanine aminotransferase (ALT) elevation(>2ULN) in TDF-treated mothers were lower in TDF-treated mothers than LdT-treated mothers (7.3% vs.15.7%, p<0.05). Alanine aminotransferase flares in TDF-treated mothers were observed lower than LdT-treated mothers (7.3% vs.15.7%, p< 0.05).Conclusions: TDF and LdT use in late pregnancy for highly viremic mothers was equally effective in reducing MTCT. Although complained more digestive system symptoms, TDF treated mothers had fewer ALT abnormalities than LdT.
The Interactive Association between Sodium Intake, Alcohol Consumption and Hypertension among Elderly in Northern China: A Cross-Sectional Study
Authors: Nan Xi , Lu Haiwen , Wu Jing , Xue Mingming , Qian Yonggang , Wang Wenrui , Wang Xuemei 
Published: November 2020

Abstract
Background: Hypertension is a worldwide public health problem. We sought to examine the interactive association between sodium intake, alcohol consumption and hypertension among elderly residents of Inner Mongolia in Northern China.Methods: This cross-sectional study used the National Survey Data for Nutrition and Adult Chronic Disease in Inner Mongolia. The prevalence of hypertension was age standardized by direct method. Sodium intake and alcohol consumption were estimated using a weighing method and 24-hour recalls over three consecutive days. Hypertension was either self-reported or field-measured. Participants were categorized into six subgroups according to the sodium intake status and drinking levels. Logistic regression was used to determine the interactive effects between sodium intake and drinking on hypertension.Results: A total 820 elderly participated in the study, of whom 523 (63.80%, age-standardized rate 62.33%) had been diagnosed with hypertension. The mean sodium intake was 4.88g. Sodium intake and drinking excessively were both independently related to a higher risk of hypertension. Adjusting for confounders, there was interaction between sodium intake and alcohol consumption in the six subgroups, with the risk of hypertension being highest among the group with excessive sodium intake and excessive alcohol consumption, with an odds ratio of 3.639 (95% confidence interval: 1.666–7.947).Conclusions: The study highlights the interactive association between sodium intake and alcohol consumption with hypertension. Primary healthcare providers should take special consideration of those who are older age with hypertension in Inner Mongolia, especially those with an unhealthy diet including both excessive sodium and excessive alcohol intake.
Telemedical care improves quality of life in patients with schizophrenia and bipolar disorder. Results of a randomized controlled trial
Authors: Stentzel Ulrike , Berg Neeltje van den , Moon Kilson , Strobel Lara N. , Schulte Josephine , Langosch Jens M. , Hoffmann Wolfgang , Grabe Hans J. 
Published: November 2020

Abstract
Background: Schizophrenia and bipolar disorder are serious psychiatric disorders with a high disease burden, a high number of years of life lived with disability and a high risk for relapses and re-hospitalizations. Besides, both diseases are often accompanied with a reduced quality of life. A low level of quality of life is one predictor for relapses. This study examines whether a telemedical care program can improve quality of life.Methods: Post stationary telemedical care of patients with severe psychiatric disorders” (Tecla) is a prospective controlled randomized intervention trial to implement and evaluate a telemedical care concept for patients with schizophrenia and bipolar disorder. Participants were randomized to an intervention or a control group. The intervention group received telemedical care including regular, individualized telephone calls and SMS-messages. The quality of life was measured with the German version of the WHOQOL-BREF. Effects of telemedicine on quality of life after 6 months were analyzed using t-tests to compare the intervention with the control group. Participants also evaluated the telemedical care program based on a short standardized interview.Results: 118 participants were recruited, thereof 57.6 % men (n = 68). Participants were on average 43 years old (SD) 13). Linear mixed model revealed that affiliation to patient group (0 = CG, 1 = IG), gender (0 = female, 1 = male), increasing social support and higher GAF-level are positive significant influence factors for the WHOQOL total quality of life, physical, psychological, environmental and global domain. An increasing education often showed significantly decreasing quality of life values. Age as an influencing factor, showed different results on the sum score and the individual domains.Conclusion: The Tecla telemedical care concept has improved the quality of life in patients with severe psychiatric disorders. It provides for a low-threshold and well suitable component in psychiatric treatment.Trial registration: German Clinical Trials Register, DRKS00008548, registered 21 May 2015 – retrospectively registered, https://www.drks.de/drks_web/setLocale_EN.do
Novel mechanism of HIV elite control by enriching gut dipeptides as HIV-1 antagonist but Prevotella agonist
Authors: Sperk Maike , Ambikan Anoop , Ray Shilpa , Singh Kamlendra , Mikaeloff Flora , Diez Rafael Ceña , Narayanan Ashwathy , Vesterbacka Jan , Nowak Piotr , Sönnerborg Anders , Neogi Ujjwal 
Published: November 2020

Abstract
HIV-1 Elite controllers (EC) are a rare group among HIV-1-infected individuals who can naturally control viral replication for a prolonged period. Due to their heterogeneous nature, no universal mechanism could be attributed to the EC status; instead, several host and viral factors are discussed for playing a role. In this study, we investigated the fecal metabolome and microbiome in a Swedish cohort of EC, treatment-naïve viremic progressors (VP) and HIV-negative individuals (HC). We observed an enrichment of dipeptides in EC compared to the other two study groups. In vitro analyses identified anti-HIV-1 properties for two dipeptides that could bind to the HIV-1 gp120. Furthermore, these dipeptides supported bacterial growth of the genus Prevotella in vitro that was enriched in EC, which influences host metabolism. Thus, increased levels of both dipeptides and Prevotella could provide beneficial effects for EC. These findings open new possibilities to develop therapeutics against HIV-1.
METTL3 Promotes Pancreatic Tumor Progression Through Regulating the miR-196a/CPEB3 Axis
Authors: Ge Pingping , Fan Dong , He Lei , Wu Qiong , Sun Jin , Xu Li Jian , Xie Gang Wei 
Published: November 2020

Abstract
Background: Methyltransferase-like 3(METTL3)-mediated N6-methyladenosine (m6A) modification has been reported to regulate microRNAs maturation. Here, the study was designed to investigate the regulatory effect of m6A-dependent miRNA maturation on pancreatic cancer progression which is still limited before.Results: We found that METTL3 significantly upregulated in the pancreatic tumor tissues. Overexpression of METTL3 promoted cancer cell proliferation and migration in vitro and tumor progression in vivo. METTL3-mediated m6A modification facilitated miR-196a maturation in pancreatic cancer cells, and miR-196a increased the proliferation and migration of cancer cells in vitro. Luciferase reporter assay verified that cytoplasmic polyadenylation element binding protein 3 (CPEB3) was a direct target gene of miR-196a. In vivo studies proved that overexpression of miR-196a inhibited the anti-tumor effect of knockdown of METTL3, and overexpression of CPEB3 inhibited the miR-196a-enhanced tumor progression. Conclusions: We identified that METTL3 was upregulated in pancreatic cancer, leading to the upregulation of miR-196a, resulting in the downregulation of CPEB3, which promoted the pancreatic tumor progression. We first demonstrated that CPEB3 was a tumor suppressor gene in pancreatic cancer, and the METTL3 regulated miR-196a/CPEB3 axis may be a therapeutic target for pancreatic cancer therapy.
In Silico Metabolic Modeling Reveals Muscle Metabolite Markers of Insulin Resistance In Newly Diagnosed Diabetic Patients
Authors: Khoshnejat Maryam , Banaei-Moghaddam Ali Mohammad , Kavousi Kaveh , Moosavi-Movahedi Ali Akbar 
Published: November 2020

Abstract
BackgroundType 2 diabetes mellitus (T2DM) is a challenging and globally ubiquitous metabolic disease caused by insulin resistance. Skeletal muscle is the major insulin-sensitive tissue that plays a great role in blood sugar homeostasis. Dysfunction of muscle metabolism is implicated in the disturbance of glucose hemostasis and the development of insulin resistance and T2DM. Here, we attempted to find metabolic dysregulations that are associated with the onset of T2DM. Besides, metabolite markers of T2DM were explored. ResultsWe reconstructed a human muscle-specific metabolic model and applied it to perform metabolic analysis in newly diagnosed T2DM patients. We investigated the metabolism reprogramming by using two topology-based and constraint-based approach. Our results showed that metabolic alterations have occurred in carbohydrate, fatty acids, lipids, amino acids, and inositol phosphate metabolisms as well as pathways implicated in building extracellular matrix (ECM). Also, dysregulation of coenzyme Q10 metabolism was observed. Moreover, we applied a machine learning method to predict potential metabolite markers of insulin resistance in muscle. 13 exchange metabolites were predicted as the potential metabolite markers of insulin resistance in skeletal muscle. The efficiency of these markers in detecting insulin-resistant muscle was validated using a separate muscle gene expression data from another diabetes-related study. ConclusionIn this study, the most updated muscle-specific metabolic model was generated and successfully was validated. This model was used for the investigation of metabolic disturbances at the onset of T2DM. Our results indicated the significance of ECM metabolites in insulin resistance, and reinforce the role of coenzyme Q10 as a candidate for further research in insulin resistance and T2DM treatment. The model is freely available and can be used for other muscle metabolic studies.
MicroRNA-338-3p as A Novel Therapeutic Target for Intervertebral Disc Degeneration
Authors: Jiang Hua , Moro Abu , Wang Jiaqi , Meng Dihua , Zhan Xinli , Chen Di , Wei Qingjun 
Published: November 2020

Abstract
Background: Recent studies have demonstrated the pivotal role played by microRNA (miRNA) in the etiopathogenesis of intervertebral disc degeneration (IDD). The aim of this current study is to investigate whether intra-discal delivery of miRNA can attenuate IDD development.Results: miR-338-3p expression was significantly increased in the nucleus pulposus (NP) of patients with IDD. Moreover, there was a statistically significant positive correlation between the level of expression of miR-338-3p and the severity of IDD. Our functional studies showed that miR-338-3p significantly influenced the expression of extracellular matrix synthesis of genes, as well as the proliferation and apoptosis of NP cells. Mechanistically, miR-338-3p aggravated IDD progression by directly targeting SIRT6, a negative regulator of MAPK/ERK pathway. Intra-discal injection of antagomir-338-3p significantly decelerated IDD development in mice models.Conclusions: miR-338-3p is identified as a mediator of IDD, and thus may be a promising target for rescuing IDD.
Knockdown of PC4 Increases Chemosensitivity of Oxaliplatin in Triple Negative Breast Cancer by Supressing mTOR Pathway
Authors: Wang Qing , Ma Le , Chen Long , Chen Hongdan , Luo Min , Yang Wei , Liao Fengying , Gong Qiang , Wang Yang , Yang Zeyu , Wu Jie , Zhang Can , Zheng Jiancheng , Han Shiqian , Leng Yu , Luo Peng , Chunmeng Shi 
Published: November 2020

Abstract
Background: The response to chemotherapy is an important factor in the prognosis of patients with triple negative breast cancer (TNBC). Human positive coactivator 4 (PC4) is a multifunctional nuclear protein, that is highly expressed in various tumors including breast cancer and shows a potential role in cancer development and progression. However, the role of PC4 in chemotherapeutic responses of TNBC remains unclear. Our work aim to identify the role of PC4 in the response to oxaliplatin (Oxa) of TNBC patients.Results: We found that PC4 is significantly upregulated in TNBC cells compared with non-TNBC cells, implying its potential role in TNBC. Then, in vivo and in vitro studies revealed that knockdown of PC4 increased chemosensitivity of Oxa in TNBC by supressing mTOR pathway.Conclusions: Our findings demonstrated the signatures of PC4 in the chemotherapeutic response of TNBC, and indicated that PC4 might be a promising therapeutic target for TNBC.
Phylogenetic and Functional Analysis of CesA Genes in Cotton
Authors: Cui Zhuikui , Sun Guiqin , Zhao Quanzhi 
Published: November 2020

Abstract
Background: Cotton (Gossypium hirsutum) is widely distributed all over the world, and improving the quality of its fiber is one of the most important tasks in cotton breeding. Cotton fibers are primarily composed of cellulose, which is synthesized and regulated by cellulose synthase (CesAs). But the molecular mechanism of CesA genes in cotton was unclear. Results: In this study, the phylogenetic history and purifying selection of CesA genes were investigated along with their functions. CesA3 and CesA6 are the two largest subgroups in G. arboreum, comprising 52.8% of the whole CesA family. These two CesA subgroups were then chosen for further research, and the results showed that they are highly differentiated in dicot groups. The two subgroups were also discriminated with the use of a Ka/Ks analysis. This indicated that they may play an important role in fiber development based on their unique phylogenetic status. Functional studies were subsequently conducted using the most purified genes (Gohir.A08G144300.1 in CesA3 subgroup). The silencing of Gohir.A08G144300.1 visibly inhibits the growth of cotton fiber, showing that it is critical for the growth of cotton fibers. Conclusions: The results presented here a target gene Gohir.A08G144300.1 based on the analysis of CesA gene members, and it is found that this gene was crucial to the growth of cotton fibers. This study provides more information for the understanding of the molecular mechanism of cotton fiber development.
Source–Sink Manipulations Differentially Affected Carbon and Nitrogen Dynamics, Fruit Metabolites and Yield of Sacha Inchi Plants
Authors: Cai Zhiquan , Xie Tao , Xu Jin 
Published: November 2020

Abstract
Background: Being a promising tropical woody oilseed crop, the evergreen and recurrent plants of Sacha Inchi (Plukenetia volubilis L.) has complex phenology and source–sink interactions. Carbon source–sink manipulations with control and two treatments (reduce source, ca. 10% mature leaf pruning; reduce sink, 10% fruitlet thinning) were conducted on 2.5-year-old field-grown P. volubilis plantation during the early-wet season in a seasonal tropical area.Results: Leaf photosynthetic rate and specific leaf area largely remained unchanged in response to defoliation or defloration. Compared with control, higher N contents on average were observed in both remaining leaves and branches of the defoliated plants, suggesting that N-mobilization was mainly due to the enhanced N uptake from soil. Carbon, but not N, is a source-driven growth process of P. volubilis plants, as defoliation reduced the contents of non-structural carbohydrates (especially sugar) in branches, although temporally, whereas defloration increased available C reserve. The seasonal dynamic pattern of fruit ripening was altered by source–sink regulations. The total seed yield throughout the growing season that depends on fruit set and retention (i.e., number of matured fruit) rather than individual fruit development (size), was slightly increased by defloration but was significantly decreased by defoliation. Compared with control, defloration did not enrich the KEGG pathway, but defoliation downregulated the TCA cycle and carbohydrate and lipid metabolisms in fruitlets after 24 days of the applications of source–sink manipulation. Conclusion: Carbohydrate reserves served to buffer sink–source imbalances that may result from temporary adjustment in demand for assimilates (e.g., defloration) or shortfalls in carbon assimilation (e.g., defoliation). Defoliation is disadvantageous for the yield and also for carbohydrate and lipid accumulation in fruits of P. volubilis plants. Although more studies are needed, these results provide new insights to the further improvement in seed yield of the strong source-limited P. volubilis plants by source/sink manipulations.
Genome-wide identification of the Capsicum bHLH transcription factor family: discovery of candidate regulator involved in the regulation of species-specific bioactive metabolites
Authors: Liu Renjian , Song Jiali , Liu Shaoqun , Chen Changming , Zhang Shuanglin , Wang Juntao , Cao Bihao , Zhu Zhangsheng , Lei Jianjun 
Published: November 2020

Abstract
Background: The basic helix–loop–helix (bHLH) transcription factors (TFs) serve crucial roles in the regulation of plant growth and development and usually participate in biological processes by interacting with other TFs. Capsorubin and capsaicinoids are found only in Capsicum, which has high nutritional and economic value. However, whether bHLH family genes regulate capsorubin and capsaicinoid biosynthesis and participate in these processes by interacting with other TFs remains to be determined.Results: In this study, a total of 107 CabHLHs were identified from the Capsicum annuum genome. Phylogenetic tree analysis revealed that these CabHLH proteins were classified into 15 groups by comparing the CabHLH proteins with Arabidopsis bHLH proteins. A transcriptome analysis showed that some CabHLHs might be associated with the regulation of capsorubin and capsaicinoid biosynthesis, and the CabHLHs were focused mainly in cluster C1, cluster C2, cluster C3, cluster C4, cluster L5, cluster L6, cluster L8 and cluster L9. In cluster C1, cluster C2, and cluster C3, the expression profiles of CabHLH009, CabHLH032, CabHLH048, CabHLH095 and CabHLH100 were similar to the pattern of carotenoid biosynthesis in pericarp, including β-carotene, zeaxanthin, lutein and capsorubin, while the expression profiles of CabHLH007, CabHLH009, CabHLH026, CabHLH063 and CabHLH086 found in cluster L5, cluster L6 and cluster L9 were consistent with the pattern of capsaicin accumulation in the placenta. CabHLH007, CabHLH009, CabHLH026 and CabHLH086 also might be involved in temperature-mediated capsaicinoid biosynthesis. Additionally, yeast two-hybrid (Y2H) assays demonstrated that CabHLH007, CabHLH009, CabHLH026, CabHLH063 and CabHLH086 could interact with MYB31, a master regulator for capsaicinoid biosynthesis.Conclusions: The comprehensive and systematic analysis of CabHLH TFs provides significantly useful information that contributes to further investigation of CabHLHs in carotenoid and capsaicinoid biosynthesis.