bluuebunny/crossref_metadata_2025_split huggingface dataset:
Saliva proteomic analysis reveals possible biomarkers of renal cell carcinoma
Authors: Zhang Xiao Li , Wu Zheng Zhi , Xu Yun , Wang Ji Guo , Wang Yong Qiang , Cao Mei Qun , Wang Chang Hao 
Published: August 2020

AbstractEarly diagnosis is a key to improve the prognosis of renal cell carcinoma (RCC); however, reliable RCC biomarkers are lacking in clinical practice. In this study, we used isobaric tags for relative and absolute quantification-based mass spectrometry to identify salivary proteins as biomarkers for the diagnosis of RCC. The objective of this study is to discover biomarkers from saliva by utilizing high-throughput quantitative proteomics approaches. Saliva proteins from 124 RCC patients and healthy individuals were identified and quantified. RCC putative biomarkers were verified by real-time polymerase chain reaction or enzyme-linked immunosorbent assay in a prevalidation sample set. Seventy-one differentially expressed salivary proteins were identified. Serotransferrin, haptoglobin, KRT9, and S100A9, which in previous studies were found to be most closely related to cancers, were selected as putative RCC biomarkers. Haptoglobin and S100A9 were significantly elevated in RCC compared with healthy control samples, although the expression of serotransferrin and KRT9 did not differ between the groups. Furthermore, receiver operating characteristic curves with a cut-off value of 75.49 ng/mL for S100A9 revealed a sensitivity of 87.10% and a specificity of 91.94% for discriminating RCC patients from healthy individuals. Salivary haptoglobin differentiated RCC patients from healthy controls with a sensitivity of 85.48% and specificity of 80.65% (cut-off value 43.02 µg/mL). These results provide experimental evidence to support S100A9 and haptoglobin as potential novel, noninvasive biomarkers for the diagnosis of RCC.
Withania frutescens: Chemical characterization, analgesic, anti-inflammatory, and healing activities
Authors: EL Moussaoui Abdelfattah , Jawhari Fatima Zahra , Bourhia Mohammed , Maliki Imane , Sounni Fatiha , Mothana Ramzi A. , Bousta Dalila , Bari Amina 
Published: August 2020

AbstractWithania frutescens (W. frutescens) is a medicinal plant that is largely used in the Morrocan pharmacopeia for disease treatment. This work was conducted to investigate the chemical characterization, analgesic, anti-inflammatory, and healing activities of W. frutescens. The chemical characterization of W. frutescens extract was done using HPLC; the anti-inflammatory test was performed with doses 300, 400 and 450 mg/kg, and the healing activity was assessed using two creams (extract 5% and extract 10%). Phytochemical analysis revealed the presence of phenolic compounds. The results of the anti-inflammatory test were more pronounced when compared with the reference drug with a maximum inhibition percentage of 82.20% ± 8.69 obtained at the dose of 450 mg/kg. Local application of 10% plant cream induced 80.17% ± 7.89 of inflammation inhibition when compared with the indomethacin drug 92.33% ± 11.27. The studied plant extract showed a promising healing activity with the following percentage: 99.03% ± 0.76 (extract 10%), 98.61% ± 1.91 (extract 5%), and 57.43% ± 2.97 (control); meanwhile, the value reached to 100% ± 0.02 for the drug that was used as a reference within the first 2 weeks. The plant studied in this work would be a promising source for conceptualizing effective drugs against inflammatory diseases.
Development and validation of a high performance liquid chromatography/diode array detection method for estrogen determination: Application to residual analysis in meat products
Authors: Alqahtani Sadeem S. , Bin Humaid Deema M. , Alshail Sabreen H. , AlShammari Dalal T. , Al-Showiman Hessa , Alzoman Nourah Z. , Maher Hadir M. 
Published: August 2020

AbstractIn this work, an HPLC-DAD method was developed for the residual analysis of some estrogens such as estrone (E1), 17-β estradiol (E2), estriol (E3), natural estrogens, and 17-α ethinylestradiol (E4), an exoestrogen, in meat samples of different categories (chicken, n = 155, beef, n = 124, sheep, n = 122, and camels, n = 40), collected from the Saudi Market. Although banned, the use of E4 as a growth promoter in the black market is still encountered. Symmetry C18 column (3.5 µm, 4.6 mm × 150 mm) was used with a mobile phase consisting of 50% aqueous acetonitrile. Protein precipitation with acetonitrile was used for the sample preparation. The method was fully validated, as per the ICH guidelines, in the concentration ranges of 0.35–125 µg/g (E1, E2), 0.188–125 µg/g (E3), and 0.188–450 µg/g (E4). The method allowed the trace analysis of estrogens with LOD values of 0.094 (E3, E4) and 0.126 µg/g (E1, E2), and LOQ values of 0.188 (E3, E4) and 0.350 µg/g (E1, E2). The analyzed samples contained different levels of estrogens. Within the same category, processed products contained the highest levels of E4, while the internal organs contained the least estrogen content. Finally, the estimated daily intake, µg/kg bw/day, of estrogens through the consumption of meat-based food products was calculated.
A theoretical and experimental study of the adsorptive removal of hexavalent chromium ions using graphene oxide as an adsorbent
Authors: Alija Ardhmeri , Gashi Drinisa , Plakaj Rilinda , Omaj Admir , Thaçi Veprim , Reka Arianit , Avdiaj Sefer , Berisha Avni 
Published: August 2020

AbstractThis study is focused on the adsorption of hexavalent chromium ions Cr(vi) using graphene oxide (GO). The GO was prepared by chemical oxidation (Hummers method) of graphite particles. The synthesized GO adsorbent was characterized by Fourier transform infrared spectroscopy and UV-Vis spectroscopy. It was used for the adsorption of Cr(vi) ions. The theoretical calculations based on density functional theory and Monte Carlo calculations were used to explore the preferable adsorption site, interaction type, and adsorption energy of GO toward the Cr(vi) ions. Moreover, the most stable adsorption sites were used to calculate and plot noncovalent interactions. The obtained results are important as they give molecular insights regarding the nature of the interaction between GO surface and the adsorbent Cr(vi) ions. The found adsorption energy of −143.80 kcal/mol is indicative of the high adsorptive tendency of this material. The adsorption capacity value of GO toward these ions is q = 240.361 mg/g.
Optimisation of urine sample preparation for shotgun proteomics
Authors: Tkáčiková Soňa , Talian Ivan , Sabo Ján 
Published: August 2020

AbstractUrine reflects the renal function and urinary and kidney systems, but it may also reflect the presence of cancer in other parts of the body. Urine also has potential for providing prognostic information during therapeutic treatments thanks to non-invasive monitoring. A quick and reproducible protein purification procedure is essential to allow data comparison between proteomic studies in urine biomarker discovery. The article describes a simple, reproducible and cheap sample preparation procedure with a maximum protein yield (400 µg) obtained from only 10 mL of urine utilising cut-off filter desalting and digestion. The reported procedure removes yellowish background coloration residues and thus prevents the errors in spectrophotometric protein concentration determination. Different extraction solvents used in the presented procedure point to the possibility of partial elimination of abundant proteins (albumin and keratin family), as well as to the improvement of the sequence coverage of proteins identified, which helps to reveal changes in the urinary proteome. With this workflow, proteins can be easily obtained on standard laboratory equipment within 3 h. Data are available via ProteomeXchange with identifier PXD019738.
H/D isotope effect and magnetic properties of cyanide-bridged Nd(III)-Fe(III) complex
Authors: Akitsu T. , Kimoto Y. , Yamada Y. , Nomura K. 
Published: August 2020

Abstract
We have prepared four H/D isotope forms of Nd(DMF)4(H2O)3Fe(CN)6·H2O (DMF = N,N-dimethylformamide) by using D2O or DMF-d1. Temperature dependence of magnetization exhibits H/D isotope effects resulting from intermolecular hydrogen bonds. Temperature dependence of Fe 2p
3/2 and 2p
1/2 XAS and 57Fe Mössbauer spectra suggested that the influence of coordination environment could be distiguished from the influence of crystal lattice based on preliminary crystallographic results.
Meiotic Double-Strand Break Processing and Crossover Patterning Are Regulated in a Sex-Specific Manner by BRCA1–BARD1 in Caenorhabditis elegans
Authors: Li Qianyan , Hariri Sara , Engebrecht JoAnne 
Published: August 2020

Abstract
Sperm and oocyte production are differentially regulated to ensure genetic information is accurately passed down from one generation to the next.....
Ethanolic Extract of Xestospongia Sp. Induces CD4+ and CD14 Cells Levels on Wistar Male Rat Infected with Staphylococcus aureus
Authors: Fristiohady Adryan , Wahyuni Wahyuni , Malaka Muhammad H , Madu Dewiyanti , Muthalib Dayatriana , Munasari Dian , Purnama La Ode MJ , Sadarun Baru , Ilyas Muhammad , Sahidin Idin 
Published: August 2020

Immunomodulator is a substance that increases or suppresses the immune response through the certain mechanism. The marine sponge Xestospongia sp. has immunomodulatory activity by increasing phagocytic activity. In addition, the phagocytic activity is affected by CD4+ and CD14 cells levels. Thus, this study aims to investigate the effect of Xestopongia sp. extract toward CD4+ and CD14 cells level in model rat. Animals were divided into 4 groups (n=5) and treated for 7 days, as follow: Group I (Ethanolic extract of Xestospongia sp. dose of 300 mg/KgBW); Group II (Ethanolic extract of Xestospongia sp. dose of 400 mg/KgBW); Group III (Phylantus niruri extract); and Group IV (0.5% Na CMC). On day 8, animals were infected with Staphylococcus aureus intraperitoneally. And the blood was collected by cardiac puncture and assayed with ELISA kit CD4 (elabscience®) and ELISA kit CD14 (elabscience®). Ethanolic extract of Xestospongia sp. provided high levels of both CD4+ and CD14 cells (Group II) compared to baseline (Group IV) (p<0.05). Group I provided similar activity to group III (p>0.05) and Group II provided significant activity with higher levels of CD4+ and CD14 cells compared to group III (p<0.05). In conclusion, both doses of Xestospongia sp. extract provide activity as immunomodulator by increasing CD4+ and CD14 cells levels, yet dose of 400 mg/KgBw provides the higher immunomodulatory activity.
PLANEJAMENTO DO TRANSPORTE CANAVIEIRO A PARTIR DE SISTEMA DE INFORMAÇÃO GEOGRÁFICA
Authors: Harmyans Moreira Alisson Neves , Griebeler Nori Paulo , Himmen Fernanda Duarte Araujo , Silva Almeida Rherison Tyrone 
Published: August 2020

PLANEJAMENTO DO TRANSPORTE CANAVIEIRO A PARTIR DE SISTEMA DE INFORMAÇÃO GEOGRÁFICA*

ALISSON NEVES HARMYANS MOREIRA1, NORI PAULO GRIEBELER1, FERNANDA DUARTE ARAÚJO HIMMEN 1, RHERISON TYRONE SILVA ALMEIDA2

1 Escola de Agronomia, EA-UFG, Campus Samambaia, Rodovia Goiânia/Nova Veneza, km 0, CEP 74690-900, Goiânia, GO, Brasil, 1alissonharmyans@gmail.com, 2npgriebeler@gmail.com, 3fernanduarteflorestal@gmail.com.
2 Instituto de Estudos Socioambientais, IESA-UFG, avenida Esperança s/n, Campus Samambaia, CEP 74001-970, Goiânia, GO, Brasil.
* Produção extraída da dissertação do primeiro autor.

RESUMO: Elaboraram-se por meio de Sistema de Informação Geográfica (SIG), roteirizações para o transporte canavieiro, tendo em vista a análise de variáveis de dois cenários, o menor caminho e o que oferece melhor desempenho operacional. Na comparação de critérios, os resultados não diferiram significativamente pelo teste t student (σ < 0,05). Os dados subsidiaram a avaliação do custo de reparo e manutenção (CRM) em relação a vida útil e por quilometragem. Foram verificados que as discrepâncias entre os custos tornam-se expressivas a partir da quarta safra chegando a diferença de R$ 52.712,23. Assim, tendo em vista a logística da usina, inferiu-se que o critério do desempenho operacional é o ideal para a frota da empresa, enquanto o menor caminho é a melhor opção para o pagamento do serviço terceirizado.

Palavras-chaves: cana-de-açúcar, logística, estradas não pavimentadas.

SUGARCANE TRANSPORTATION PLANNING FROM A GEOGRAPHIC INFORMATION SYSTEM (GIS)

ABSTRACT: Through the Geographic Information System (GIS), routes for sugarcane transport were elaborated, in view of variable analysis from two scenarios, the shortest path and the one that offers the best operational performance. In the criteria comparison, the results did not differ significantly by the student t test (σ <0.05). The data supported the assessment of the repair and maintenance cost (CRM) in relation to service life and mileage. It was found that the discrepancies between costs become significant as the fourth harvest, reaching a difference of R$ 52,712.23. Thus, in a view of plant logistics, it was inferred that operational performance criterion is ideal to company fleet, while the shortest route is the best option for paying outsourced service.

Keywords: sugarcane, logistic, unpaved roads.
Probabilistic Moment Tensor Inversion for Hydrocarbon-Induced Seismicity in the Groningen Gas Field, the Netherlands, Part 2: Application
Authors: Dost Bernard , van Stiphout Annemijn , Kühn Daniela , Kortekaas Marloes , Ruigrok Elmer , Heimann Sebastian 
Published: August 2020

ABSTRACTRecent developments in the densification of the seismic network covering the Groningen gas field allow a more detailed study of the connection between induced seismicity and reactivated faults around the gas reservoir at 3 km depth. With the reduction of the average station distance from 20 km to 4–5 km, a probabilistic full-waveform moment tensor inversion procedure could be applied, resulting in both improved hypocenter location accuracy and full moment tensor solutions for events of M≥2.0 recorded in the period 2016–2019. Hypocenter locations as output from the moment tensor inversion are compared to locations from the application of other methods and are found similar within 250 m distance. Moment tensor results show that the double-couple (DC) solutions are in accordance with the known structure, namely normal faulting along 50°–70° dipping faults. Comparison with reprocessed 3D seismic sections, extended to a depth of 6–7 km, demonstrate that (a) most events occur along faults with a small throw and (b) reactivated faults in the reservoir often continue downward in the Carboniferous underburden. From non-DC contributions, the isotropic (ISO) component is dominant and shows consistent negative values, which is expected in a compacting medium. There is some indication that events connected to faults with a large throw (&gt;70  m) exhibit the largest ISO component (40%–50%).
Regional-Scale 3D Ground-Motion Simulations of Mw 7 Earthquakes on the Hayward Fault, Northern California Resolving Frequencies 0–10 Hz and Including Site-Response Corrections
Authors: Rodgers Arthur J. , Pitarka Arben , Pankajakshan Ramesh , Sjögreen Bjorn , Petersson N. Anders 
Published: August 2020

ABSTRACTLarge earthquake ground-motion simulations in 3D Earth models provide constraints on site-specific shaking intensities but have suffered from limited frequency resolution and ignored site response in soft soils. We report new regional-scale 3D simulations for moment magnitude 7.0 scenario earthquakes on the Hayward Fault, northern California with SW4. Simulations resolved significantly broader band frequencies (0–10 Hz) than previous studies and represent the highest resolution simulations for any such earthquake to date. Seismic waves were excited by a kinematic rupture following Graves and Pitarka (2016) and obeyed wave propagation in a 3D Earth model with topography from the U.S. Geological Survey (USGS) assuming a minimum shear wavespeed, VSmin, of 500  m/s. We corrected motions for linear and nonlinear site response for the shear wavespeed, VS, from the USGS 3D model, using a recently developed ground-motion model (GMM) for Fourier amplitude spectra (Bayless and Abrahamson, 2018, 2019a). At soft soil locations subjected to strong shaking, the site-corrected intensities reflect the competing effects of linear amplification by low VS material, reduction of stiffness during nonlinear deformation, and damping of high frequencies. Sites with near-surface VS of 500  m/s or greater require no linear site correction but can experience amplitude reduction due to nonlinear response. Averaged over all sites, we obtained reasonable agreement with empirical ergodic median GMMs currently used for seismic hazard and design ground motions (epsilon less than 1), with marked improvement at soft sedimentary sites. At specific locations, the simulated shaking intensities show systematic differences from the GMMs that reveal path and site effects not captured in these ergodic models. Results suggest how next generation regional-scale earthquake simulations can provide higher spatial and frequency resolution while including effects of soft soils that are commonly ignored in scenario earthquake ground-motion simulations.
Desarrollo de competencias científicas mediante una unidad didáctica de ecosistemas en grado sexto
Authors: Coronado Peña Jhorman Jesid , Builes González Yeliza , Vargas Orozco Ángela Patricia , Obando-Correal Nadia Lucía 
Published: August 2020

El artículo tiene como propósito presentar los resultados de una investigación orientada al fortalecimiento de competencias científicas en estudiantes mediante una unidad didáctica de ecosistemas en grado sexto. EL estudio de naturaleza mixta aborda un componente cuantitativo a través de la aplicación de un cuestionario a los estudiantes para validar conocimientos con relación al tema planteado; igualmente se trabaja un componente cualitativo a través de la Investigación-Acción-Participativa (IAP); se exploran las ideas previas de los estudiantes a través de preguntas vinculadas con el mundo de la vida, posteriormente se realiza una intervención didáctica que permita la conceptualización y construcción de saberes científicos; finalmente se hace una valoración del proceso de aprendizaje a través de autoevaluación y heteroevaluación de lo conceptual, procedimental y actitudinal. Como resultado se evidencia el desarrollo de competencias del pensamiento científico como la observación de fenómenos particulares y su registro, formulación de hipótesis, análisis de resultados, generación de conclusiones y trabajo en equipo. Se concluye que las actividades experienciales permiten al estudiante ubicarse desde el rol de científico cuando lo invitan a que observe, registre y genere conclusiones, efectivamente promueven esas competencias del pensamiento científico.
Summary of Agricultural Application of Radar Remote Sensing
Authors: Li Sicheng 
Published: August 2020

<p>Radar remote sensing has the ability of all-day and all-weather monitoring, has certain penetration ability to vegetation, and is sensitive to the shape, structure and dielectric constant of vegetation scatterers. These characteristics make it have great potential in agricultural application. Firstly, this paper introduces the application fields of radar remote sensing in agriculture, and summarizes the current research literature in many fields, such as crop identification and classification, farmland soil moisture inversion, crop growth monitoring and so on. Then, the application status and research achievements of radar scatterometer and various SAR features (including SAR backscattering features, polarization features, interference features and tomography features) in various fields of agriculture are described respectively. Finally, the problems and reasons existing in the current research are summarized according to the agricultural application requirements and the development of SAR technology, and the future development is prospected.</p><p align="justify"> </p>
Eclampsia y Retina
Authors: Tribín Piedrahita Alfonso 
Published: August 2020

Hace poco se considereba un privilegio del oftalmólogo, el examinar el fondo del ojo. Este concepto ha sido revaluado en buena hora, y hoy son numerosos los profesionales que rutinariamente practican este examen, no con el objeto de invadir los dominios del especialista, sino precisamente, para ordenar un examen completo y una indicación terapéutica por parte de éste, al menor síntoma de alteración ocular en el curso de una enfermedad general.
Hodgkin Pélvico
Authors: López Escobar Guillermo 
Published: August 2020

Enfermedad actual: Desde hace unos cuatro meses comenzó a notar dolor y endurecimiento de la fosa ilíaca izquierda, habiendo aumentando los dolores y el tamaño del tumor especialmente en los dos últimos meses. Es una constipada crónica y últimamente ha tenido flujo blanco fétido, poliaquiuria y disuria.
Algunas observaciones sobre Tolserol en el parto
Authors: González Efraín 
Published: August 2020

Fue sintetizado en Inglaterra en 1946 por Berger y Brandley e inicialmente hicieron sus estudios experimentales en animales para averiguar sus propiedades farmacológicas y comprobaron que con dosis perfectamente inocuas se producía relajación muscular, lo cual indujo para que se envara en los estados caracteri­zados por espasmos, contractura. que denotan una hiperactividad muscular.
Otros investigadores concluyeron que el sitio de acción del Myanesín se verifica en la célula de conexión neuro-muscular ejerciendo una disminución de eliminando el estímulo nervioso que llega a las células de las astas anteriores de la medula espinal desde los centros nerviosos, reduciendo así, el nivel de activi­dad muscular.
Neutrophil extracellular traps: diagnostic and prognostic value in COVID-19
Authors: Kassina D. V. , Vasilenko I. A. , Gur’ev A. S. , Volkov A. Yu. , Metelin V. B. 
Published: August 2020

Rationale: An important element of antiviral defense in the pathophysiology of COVID-19 is the innate cell immunity including polymorphonuclear neutrophils prone to netotic transformation. Neutrophils can be not only a marker of acute infection, but, being a source of neutrophil extracellular traps (NET), can play a key role in the development of thrombotic complications leading to acute respiratory insufficiency in COVID-19.Aim: To determine the diagnostic and prognostic value of NET levels in patients with COVID-19.Materials and methods: We monitored NET levels in peripheral blood of 34 patients with COVID-19 (mean age, 67 ± 15.8 years), admitted to MONIKI hospital. The control group consisted of 54 healthy volunteers (mean age, 52 ± 11.5 years). Whole blood samples of 2 pL each were used for the preparation of monolayer smears (Giemsa stain) and calculation of at least 200 cell structures including native intact and transformed neutrophils (MECOS-C2 microscope, Medical computer systems).Results: Patients with COVID-19 had higher NET levels, compared to those in healthy controls: 14.5% (2.9-28.6%) vs. 5.0% (1.8-11.9%, p < 0.0001). The patients who were on non-invasive respiratory support (23.5%) had a NET level of 12% (8.122.3%), whereas those on invasive mechanical ventilation (17.6%) had a 1.5-fold higher NET level of 17.9% (12.3-28.2%) (p < 0.05). In the patients who died (11.8% of the cases), the NET level amounted to 19% (16.5-26%, p < 0.05). Monitoring of blood NET levels was performed in 9 patients from the day of admittance to the day of their transfer to the intensive care unit / discharge / death. It was shown that a decrease of NET levels mirrors an improvement of the patient's clinical condition and efficacy of his/hers treatment. On the opposite, an increase of NET levels can indicate a deterioration and risk of unfavorable course.Conclusion: We have identified some pathophysiological mechanisms in COVID-19, related to the neutrophil compartment. Patients with coronavirus infection are characterized by high NET levels which is at least 3-fold higher than that in healthy volunteers. This indicates an abnormality in immune host defense and development of an inadequate inflammatory response. An increase of NET in whole blood smears of more than 16% can be a criterion of an unfavorable prognosis of the disease course and the risk of death.