bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro antitumor, pro-inflammatory, and pro-coagulant activities of Megalopyge opercularis J.E. Smith hemolymph and spine venom
Authors: Orozco-Flores Alonso A. , Valadez-Lira José A. , Covarrubias-Cárdenas Karina E. , Pérez-Trujillo José J. , Gomez-Flores Ricardo , Caballero-Hernández Diana , Tamez-Guerra Reyes , Rodríguez-Padilla Cristina , Tamez-Guerra Patricia 
Published: October 2020

AbstractContact with stinging spines venom from several Lepidoptera larvae may result in skin lesions. In Mexico, envenomation outbreaks caused by Megalopyge opercularis were reported between 2015 and 2016. The aim of this study was to identify the venomous caterpillars in Nuevo Leon, Mexico and evaluate several biological activities of their hemolymph (HEV) and spine setae (SSV) venoms. M. opercularis was identified by cytochrome oxidase subunit (COI) designed primers. HEV and SSV extracts cytotoxic activity was assessed on the L5178Y-R lymphoma cell line. For apoptotic cells number and apoptosis, cells were stained with acridine orange/ethidium bromide and validated by DNA fragmentation. Human peripheral blood mononuclear cells (hPBMC) cytokine response to the extracts was measured by the cytometric bead array assay. Extracts effect on pro-coagulation activity on human plasma was also evaluated. HEV and SSV extracts significantly inhibited (p < 0.01) up to 63% L5178Y-R tumor cell growth at 125–500 µg/mL, as compared with 43% of Vincristine. About 79% extracts-treated tumor cells death was caused by apoptosis. Extracts stimulated (p < 0.01) up to 60% proliferation of resident murine lymphocytes, upregulated IL-1β, IL-6, IL-8, and TNF-α production by hPBMC, and showed potent pro-coagulant effects. The pharmacological relevance of these venoms is discussed.
Population genomics reveals lack of greater white-fronted introgression into the Swedish lesser white-fronted goose
Authors: Díez-del-Molino David , von Seth Johanna , Gyllenstrand Niclas , Widemo Fredrik , Liljebäck Niklas , Svensson Mikael , Sjögren-Gulve Per , Dalén Love 
Published: October 2020

AbstractInterspecific introgression is considered a potential threat to endangered taxa. One example where this has had a major impact on conservation policy is the lesser white-fronted goose (LWfG). After a dramatic decline in Sweden, captive breeding birds were released between 1981–1999 with the aim to reinforce the population. However, the detection of greater white-fronted goose (GWfG) mitochondrial DNA in the LWfG breeding stock led to the release program being dismantled, even though the presence of GWfG introgression in the actual wild Swedish LWfG population was never documented. To examine this, we sequenced the complete genomes of 21 LWfG birds from the Swedish, Russian and Norwegian populations, and compared these with genomes from other goose species, including the GWfG. We found no evidence of interspecific introgression into the wild Swedish LWfG population in either nuclear genomic or mitochondrial data. Moreover, Swedish LWfG birds are genetically distinct from the Russian and Norwegian populations and display comparatively low genomic diversity and high levels of inbreeding. Our findings highlight the utility of genomic approaches in providing scientific evidence that can help improve conservation management as well as policies for breeding and reinforcement programmes.
Mitochondrial gene signature in the prefrontal cortex for differential susceptibility to chronic stress
Authors: Weger Meltem , Alpern Daniel , Cherix Antoine , Ghosal Sriparna , Grosse Jocelyn , Russeil Julie , Gruetter Rolf , de Kloet E. Ronald , Deplancke Bart , Sandi Carmen 
Published: October 2020

AbstractMitochondrial dysfunction was highlighted as a crucial vulnerability factor for the development of depression. However, systemic studies assessing stress-induced changes in mitochondria-associated genes in brain regions relevant to depression symptomatology remain scarce. Here, we performed a genome-wide transcriptomic study to examine mitochondrial gene expression in the prefrontal cortex (PFC) and nucleus accumbens (NAc) of mice exposed to multimodal chronic restraint stress. We identified mitochondria-associated gene pathways as most prominently affected in the PFC and with lesser significance in the NAc. A more detailed mitochondrial gene expression analysis revealed that in particular mitochondrial DNA-encoded subunits of the oxidative phosphorylation complexes were altered in the PFC. The comparison of our data with a reanalyzed transcriptome data set of chronic variable stress mice and major depression disorder subjects showed that the changes in mitochondrial DNA-encoded genes are a feature generalizing to other chronic stress-protocols as well and might have translational relevance. Finally, we provide evidence for changes in mitochondrial outputs in the PFC following chronic stress that are indicative of mitochondrial dysfunction. Collectively, our work reinforces the idea that changes in mitochondrial gene expression are key players in the prefrontal adaptations observed in individuals with high behavioral susceptibility and resilience to chronic stress.
Message framing and self-conscious emotions help to understand pro-environment consumer purchase intention: an ERP study
Authors: Zubair Muhammad , Iqbal Sidra , Usman Sardar Muhammad , Awais Muhammad , Wang Ruining , Wang Xiaoyi 
Published: October 2020

AbstractMessage framing plays an important role in advertising strategies and has been studied from various perspectives in different behavioral researches. In this study Event Rated Potentials technique helped to examine the neural mechanism of message framing effect on self-conscious emotions of pride and guilt regarding consumer purchase intention in the context of green marketing. Behavioral results showed that participants ranked higher on positive framing compared to negative framing. ERP results declared that N1 component was elicited by positive framed message with emotion of pride and guilt, reflecting use of attentional resources to acquire potential benefits at first stage of processing emotional information. At the second stage P2 was higher for negative framing containing pride and guilt slogan, showing more attention towards processing emotional information about potential loss. At the third stage LPP component portray that both positive and negative framing is supported by guilt emotion when processing emotional information in decision making. Our results delivered strong evidence that how purchase intention can be mediated by message framing under the pride and guilt emotions in the context of green marketing.
Course of depressive symptomatology and its association with serum uric acid in one-anastomosis gastric bypass patients
Authors: Winzer Eva , Ludvik Bernhard , Grabovac Igor , Kruschitz Renate , Schindler Karin , Prager Gerhard , Klammer Carmen , Hoppichler Friedrich , Marculescu Rodrig , Wakolbinger Maria 
Published: October 2020

AbstractThe changes in depressive symptomatology during the first year following one-anastomosis gastric bypass (OAGB) were evaluated and its association with uric acid (sUA). Fifty patients were included in this analysis. Beck Depression Inventory (BDI) for measuring depressive symptomatology, blood samples, and anthropometric measurements were assessed before (T0), at 6 (T6), and 12 months (T12) after surgery. There was a significant reduction in BDI total score at T6 (− 5.6 (95% CI − 2.1, − 9.1) points; p = 0.001) and at T12 (− 4.3 (95% CI − 0.9, − 7.9) points; p = 0.011). BMI loss was unrelated to depressive symptomatology. Patients with moderate to severe depressive symptomatology presented lower sUA levels than patients with none or minimal to mild (p = 0.028). ROC analysis revealed that sUA levels below 5.0 at T6 and 4.5 mg/dl at T12 had a prognostic accuracy for depression severity. Furthermore, delta sUA was significantly associated with delta BMI (β = 0.473; p = 0.012) and delta waist circumference (β = 0.531; p = 0.003). These findings support an improvement in depressive symptomatology in the first year postoperatively, however, without relation to BMI loss. Patients with moderate to severe depressive symptomatology presented with lower sUA levels over time. Therefore, sUA could be useful to predict moderate to severe depressive symptomatology in patients undergoing OAGB in clinical practice.
High-fat diet-induced obesity affects alpha 7 nicotine acetylcholine receptor expressions in mouse lung myeloid cells
Authors: Qi Yong , Si Dan , Zhu Li , Qi Yanan , Wu Zhuhua , Chen Dan , Yang Yunlei 
Published: October 2020

AbstractAmple evidence indicates that obesity causes dysfunctions in the lung. Previous studies also show that cholinergic anti-inflammatory pathways play crucial roles in obesity-induced chronic inflammation via α7 nicotinic acetylcholine receptor (α7nAChR) signaling. However, it remains unclear whether and how obesity affects the expressions of α7nAChR in myeloid cells in the lung. To address this question, we treated regular chow diet-fed mice or high-fat diet induced obese mice with lipopolysaccharide (LPS) or vehicle via endotracheal injections. By using a multicolor flow cytometry approach to analyze and characterize differential cell subpopulations and α7nAChR expressions, we find no detectable α7nAChR in granulocytes, monocytes and alveolar macrophages, and low expression levels of α7nAChR were detected in interstitial macrophages. Interestingly, we find that a challenge with LPS treatment significantly increased expression levels of α7nAChR in monocytes, alveolar and interstitial macrophages. Meanwhile, we observed that the expression levels of α7nAChR in alveolar and interstitial macrophages in high-fat diet induced obese mice were lower than regular chow diet-fed mice challenged by the LPS. Together, our findings indicate that obesity alters the expressions of α7nAChR in differential lung myeloid cells.
Cardiovascular disease risk assessment in patients with familial Mediterranean fever related renal amyloidosis
Authors: Romano Micol , Piskin David , Berard Roberta A. , Jackson Bradley C. , Acikel Cengizhan , Carrero Juan J. , Lachmann Helen J. , Yilmaz Mahmut I. , Demirkaya Erkan 
Published: October 2020

AbstractChronic inflammation and proteinuria is a risk factor for cardiovascular disease (CVD) in patients with chronic kidney diseases and rheumatologic disorders. Our aim was to investigate the CVD events (CVDEs) and survival between the patients with FMF-related AA amyloidosis and glomerulonephropathies (GN) to define possible predictors for CVDEs. A prospective follow-up study with FMF-amyloidosis and glomerulonephropathy (GN) was performed and patients were followed for CVDEs. Flow-mediated dilatation (FMD), FGF-23, serum lipid, hsCRP levels, BMI and HOMA were assessed. A Cox regression analysis was performed to evaluate the risk factors for CVDEs. There were 107 patients in the FMF-amyloidosis group and 126 patients with GN group. Forty-seven CVDEs were observed during the 4.2-years follow up; all 28 patients in the FMF-amyloidosis group and 14/19 patients with GN developed CVDEs before the age of 40 (p = 0.002). CVD mortality was 2.8 times higher (95% CI 1.02–7.76) in patients with FMF-amyloidosis. Across both groups, FMD and FGF23 (p < 0.001) levels were independently associated with the risk of CVDEs. Patients with FMF-amyloidosis are at increased risk of early CVDEs with premature mortality age. FGF 23, FMD and hsCRP can stratify the risk of early CVD in patients with FMF-related AA amyloidosis.
The SMOS-Derived Soil Water EXtent and equivalent layer thickness facilitate determination of soil water resources
Authors: Usowicz Boguslaw , Lukowski Mateusz , Lipiec Jerzy 
Published: October 2020

AbstractThe assessment of water resources in soil is important in understanding the water cycle in the natural environment and the processes of water exchange between the soil and the atmosphere. The main objective of the study was to assess water resources (in 2010–2013) in the topsoil from satellite (SMOS) and in situ (ground) measurements using the SWEX_PD approach (Soil Water EXtent at Penetration Depth). The SWEX_PD is a result of multiplying soil moisture (SM) and radiation penetration depth (PD) for each pixel derived from the SMOS satellite. The PD, being a manifold of the wavelength λ0 equal to 21 cm, was determined from the weekly SMOS L2 measurement data based on the real and imaginary part of complex dielectric constant. The SWEX_PD data were compared with soil water resources (WR) calculated from the sum of components derived from multiplication of soil moisture (SM) and layer thickness in nine agrometeorological stations located along the eastern border of Poland. Each study site consisted of seven neighbouring Discrete Global Grid pixels (nodes spaced at 15 km) including the central ones with agrometeorological stations. The study area included different types of soils and land covers. The agreement between the water resources obtained from the SWEX_PD and ground measurements (WR) was quantified using classical statistics and Bland–Altman's plots. Calibrated Layer Thickness (CLT = dbias) from 8 to 28 cm was obtained with a low values of bias (close to zero), limits of agreements, and confidence intervals for all the SWEX_PD, depending on the pixel location. The results revealed that the use of the SWEX_PD for assessing soil water resources is the most reliable approach in the study area. Additionally, the data from Bland–Altman plots and the equation proposed in these studies allowed calculation of the Equivalent Layer Thickness (ELT = $$d_{ei}^{SWEX}$$

d

ei

SWEX

), which corresponds to the water resources derived from the SMOS satellite at the same time as (SM) measurements performed in the agrometeorological stations. The ranges of the mean, standard deviation, minimum, maximum, and coefficient of variation (CV) of ELT among all pixels and stations were 8.28–28.7 cm, 3.27–12.66 cm, 3.03–10.87 cm, 19.23–94.97 cm, and 24.72–98.79%, respectively. The ranges of the characteristics depended on environmental conditions and their means were close to the values of the calibrated layer thickness. The impacts of soil texture, organic matter, vegetation, and their interactive effects on the differentiation and agreement of soil water resources obtained from SWEX_PD vs. data from ground measurements in the study area are discussed. Further studies are required to address the impact of the environmental factors to improve the assessment of soil water resources based on satellite SM products (retrievals).
Prediction and detection of human epileptic seizures based on SIFT-MS chemometric data
Authors: Catala Amélie , Levasseur-Garcia Cecile , Pagès Marielle , Schaff Jean-Luc , Till Ugo , Vitola Pasetto Leticia , Hausberger Martine , Cousillas Hugo , Violleau Frederic , Grandgeorge Marine 
Published: October 2020

AbstractAlthough epilepsy is considered a public health issue, the burden imposed by the unpredictability of seizures is mainly borne by the patients. Predicting seizures based on electroencephalography has had mixed success, and the idiosyncratic character of epilepsy makes a single method of detection or prediction for all patients almost impossible. To address this problem, we demonstrate herein that epileptic seizures can not only be detected by global chemometric analysis of data from selected ion flow tube mass spectrometry but also that a simple mathematical model makes it possible to predict these seizures (by up to 4 h 37 min in advance with 92% and 75% of samples correctly classified in training and leave-one-out-cross-validation, respectively). These findings should stimulate the development of non-invasive applications (e.g., electronic nose) for different types of epilepsy and thereby decrease of the unpredictability of epileptic seizures.
Cryogenic conditioning of microencapsulated phase change material for thermal energy storage
Authors: Trivedi G. V. N. , Parameshwaran R. 
Published: October 2020

AbstractMicroencapsulation is a viable technique to protect and retain the properties of phase change materials (PCMs) that are used in thermal energy storage (TES) applications. In this study, an organic ester as a phase change material was microencapsulated using melamine–formaldehyde as the shell material. This microencapsulated PCM (MPCM) was examined with cyclic cryogenic treatment and combined cyclic cryogenic heat treatment processes. The surface morphology studies showed that the shell surfaces had no distortions or roughness after cryogenic treatment. The cryogenically conditioned microcapsules exhibited diffraction peak intensity shifts and crystal structure changes. The onset of melting for the nonconditioned and conditioned microcapsules were measured to be 8.56–9.56 °C, respectively. Furthermore, after undergoing the cryogenic and heat treatment processes, the PCM microcapsules had appreciable latent heat capacities of 39.8 kJ/kg and 60.7 kJ/kg, respectively. Additionally, the microcapsules were found to have good chemical stability after the cryogenic treatment. In addition, the cryogenically conditioned microcapsules were found to be thermally stable up to 128.9 °C, whereas the nonconditioned microcapsules were stable up to 101.9 °C. Based on the test results, it is obvious that the cryogenically conditioned microcapsules exhibited good thermal properties and are very desirable for cool thermal energy storage applications.
Intravenous iron infusion as an alternative to minimize blood transfusion in peri-operative patients
Authors: Ionescu Alin , Sharma Abhinav , Kundnani Nilima Rajpal , Mihăilescu Alexandra , David Vlad Laurențiu , Bedreag Ovidiu , Săndesc Dorel , Dinu Anca Raluca , Săndesc Mihai Alexandru , Albulescu Nicolae , Drăgoi Răzvan Gabriel 
Published: October 2020

AbstractDespite the reported benefits of intravenous iron therapy (IVIT) for correcting iron deficiency anemia (IDA) before any major surgery and the evidence thereof, perioperative allogenic blood transfusion (ABT) practice is still considered as the only viable option by some clinicians worldwide. As ABT increases the likelihood of infections, cardiac complications, longer hospital stays and mortality among the patients, the practice of ABT should only be reserved for critical cases (Hb level < 7 g/dl). Timely iron studies and iron replenishment (oral/IV) of prospective surgical patients could help decrease the ABT practice, and prove beneficial from both the clinical and economic standpoint. Evidence based patient blood management guidelines should be developed and standardized for use by clinicians worldwide. These guidelines should include specific instructions on timely assessment of surgical patients for correction of their IDA by either oral iron supplementation, if time permits, or by using IVIT such as ferric carboxymaltose (FCM) in emergency surgeries and in patients with functional ID. This study was conducted to explore the clinical benefits of the timely administration of IV-FCM in iron-deficient preoperative patients during 2017–2018 and compare the results thereof with that of the ABT. Based on the IDA treatment plan of 2953 patients, 11.14% cases were administered IV FCM (Group 1), 11.58% cases received ABT (Group 2), while the remaining 77.27% of anemic cases received neither ABT nor IV FCM (Group 3). The results indicate that the IV FCM administration reduces the need for ABT and thus minimizes its associated side effects. The findings of our study concur with the favorable outcomes reported by the other similar studies.
Impaired mitochondrial medium-chain fatty acid oxidation drives periportal macrovesicular steatosis in sirtuin-5 knockout mice
Authors: Goetzman Eric S. , Bharathi Sivakama S. , Zhang Yuxun , Zhao Xue-Jun , Dobrowolski Steven F. , Peasley Kevin , Sims-Lucas Sunder , Monga Satdarshan P. 
Published: October 2020

AbstractMedium-chain triglycerides (MCT), containing C8–C12 fatty acids, are used to treat several pediatric disorders and are widely consumed as a nutritional supplement. Here, we investigated the role of the sirtuin deacylase Sirt5 in MCT metabolism by feeding Sirt5 knockout mice (Sirt5KO) high-fat diets containing either C8/C10 fatty acids or coconut oil, which is rich in C12, for five weeks. Coconut oil, but not C8/C10 feeding, induced periportal macrovesicular steatosis in Sirt5KO mice. 14C–C12 degradation was significantly reduced in Sirt5KO liver. This decrease was localized to the mitochondrial β-oxidation pathway, as Sirt5KO mice exhibited no change in peroxisomal C12 β-oxidation. Endoplasmic reticulum ω-oxidation, a minor fatty acid degradation pathway known to be stimulated by C12 accumulation, was increased in Sirt5KO liver. Mice lacking another mitochondrial C12 oxidation enzyme, long-chain acyl-CoA dehydrogenase (LCAD), also developed periportal macrovesicular steatosis when fed coconut oil, confirming that defective mitochondrial C12 oxidation is sufficient to induce the steatosis phenotype. Sirt5KO liver exhibited normal LCAD activity but reduced mitochondrial acyl-CoA synthetase activity with C12. These studies reveal a role for Sirt5 in regulating the hepatic response to MCT and may shed light into the pathogenesis of periportal steatosis, a hallmark of human pediatric non-alcoholic fatty liver disease.
Comparisons of Auditory Brainstem Responses Between a Laboratory and Simulated Home Environment
Authors: Parker Ashley , Slack Candace , Skoe Erika 
Published: October 2020

PurposeMiniaturization of digital technologies has created new opportunities for remote health care and neuroscientific fieldwork. The current study assesses comparisons between in-home auditory brainstem response (ABR) recordings and recordings obtained in a traditional lab setting.MethodClick-evoked and speech-evoked ABRs were recorded in 12 normal-hearing, young adult participants over three test sessions in (a) a shielded sound booth within a research lab, (b) a simulated home environment, and (c) the research lab once more. The same single-family house was used for all home testing.ResultsAnalyses of ABR latencies, a common clinical metric, showed high repeatability between the home and lab environments across both the click-evoked and speech-evoked ABRs. Like ABR latencies, response consistency and signal-to-noise ratio (SNR) were robust both in the lab and in the home and did not show significant differences between locations, although variability between the home and lab was higher than latencies, with two participants influencing this lower repeatability between locations. Response consistency and SNR also patterned together, with a trend for higher SNRs to pair with more consistent responses in both the home and lab environments.ConclusionsOur findings demonstrate the feasibility of obtaining high-quality ABR recordings within a simulated home environment that closely approximate those recorded in a more traditional recording environment. This line of work may open doors to greater accessibility to underserved clinical and research populations.
Reconfigurable log‐periodic dipole array on textile
Authors: Johnson Alexander D , Nichols Matthew W. , Bojja Venkatakrishnan Satheesh , Volakis John L. 
Published: October 2020

A microwave planar log‐periodic dipole array (LPDA) antenna embroidered on textiles is presented for the first time. This antenna gives a 3.33:1 impedance bandwidth with 5 dBi average gain across the 0.9–3GHz band. This multi‐layer textile antenna employs a new denim stack‐up for repeatable measurements. The presented LPDA is tested under conformal conditions, with a demonstrated ability of frequency‐dependent beam‐steering through physical manipulation. Further, the antenna is considered for body‐worn applications due to its conformal and low profile. A 2cm thick ground beef slab was used to emulate the human tissue for measurements. Overall, the presented LPDA brings forward new opportunities for several long‐distance wideband and wearable applications.