bluuebunny/crossref_metadata_2025_split huggingface dataset:
miRNA-103 promotes chondrocyte apoptosis by down-regulation of Sphingosine kinase-1 and ameliorates PI3K/AKT pathway in osteoarthritis
Authors: Li Fang , Yao Jianhua , Hao Qingqing , Duan Zheping 
Published: October 2019

Abstract
Objectives: The aim of the present study was to determine the effects of miRNA-103 on chondrocyte apoptosis and molecular mechanisms in osteoarthritis (OA) progression. Methods: The cell proliferation, apoptosis, and recovery ability were measured by cell counting kit-8 (CCK-8), flow cytometry, and wound healing assays. The interaction of miRNA-103 and Sphingosine kinase-1 (SPHK1) were determined by using luciferase reporter assay. The expression of mRNA and proteins were measured by qRT-PCR and Western blot. OA rat model was established by surgery stimulation. Results: miRNA-103 expression was significantly increased in the cartilage of OA patients and surgery-induced OA rat models. miRNA-103 transfection into primary rat chondrocytes reduced SPHK1 expression, induced apoptosis, inhibited cell proliferation, and impeded scratch assay wound closure. Moreover, expression of total AKT, and p-AKT were significantly reduced in miRNA-103-overexpressing chondrocytes while SPHK1 up-regulation increased the expression of phosphatidylinsitol-3-kinase (PI3K) and p-AKT, and reversed the proliferation suppression induced by the miRNA-103 mimic. Conclusions: Our studies suggest that miRNA-103 contributes to chondrocyte apoptosis, promoting OA progression by down-regulation of PI3K/AKT pathway through the reduction in SPHK1 activity.
ABCG5/G8: a structural view to pathophysiology of the hepatobiliary cholesterol secretion
Authors: Zein Aiman A. , Kaur Rupinder , Hussein Toka O.K. , Graf Gregory A. , Lee Jyh-Yeuan 
Published: October 2019

Abstract
The ABCG5/G8 heterodimer is the primary neutral sterol transporter in hepatobiliary and transintestinal cholesterol excretion. Inactivating mutations on either the ABCG5 or ABCG8 subunit cause Sitosterolemia, a rare genetic disorder. In 2016, a crystal structure of human ABCG5/G8 in an apo state showed the first structural information on ATP-binding cassette (ABC) sterol transporters and revealed several structural features that were observed for the first time. Over the past decade, several missense variants of ABCG5/G8 have been associated with non-Sitosterolemia lipid phenotypes. In this review, we summarize recent pathophysiological and structural findings of ABCG5/G8, interpret the structure-function relationship in disease-causing variants and describe the available evidence that allows us to build a mechanistic view of ABCG5/G8-mediated sterol transport.
RecA and DNA recombination: a review of molecular mechanisms
Authors: del Val Elsa , Nasser William , Abaibou Hafid , Reverchon Sylvie 
Published: October 2019

Abstract
Recombinases are responsible for homologous recombination and maintenance of genome integrity. In Escherichia coli, the recombinase RecA forms a nucleoprotein filament with the ssDNA present at a DNA break and searches for a homologous dsDNA to use as a template for break repair. During the first step of this process, the ssDNA is bound to RecA and stretched into a Watson–Crick base-paired triplet conformation. The RecA nucleoprotein filament also contains ATP and Mg2+, two cofactors required for RecA activity. Then, the complex starts a homology search by interacting with and stretching dsDNA. Thanks to supercoiling, intersegment sampling and RecA clustering, a genome-wide homology search takes place at a relevant metabolic timescale. When a region of homology 8–20 base pairs in length is found and stabilized, DNA strand exchange proceeds, forming a heteroduplex complex that is resolved through a combination of DNA synthesis, ligation and resolution. RecA activities can take place without ATP hydrolysis, but this latter activity is necessary to improve and accelerate the process. Protein flexibility and monomer–monomer interactions are fundamental for RecA activity, which functions cooperatively. A structure/function relationship analysis suggests that the recombinogenic activity can be improved and that recombinases have an inherently large recombination potential. Understanding this relationship is essential for designing RecA derivatives with enhanced activity for biotechnology applications. For example, this protein is a major actor in the recombinase polymerase isothermal amplification (RPA) used in point-of-care diagnostics.
Multimorbidität bei älteren psychiatrischen Patienten
Authors: Junghanns Klaus , Wetterling Tilman 
Published: October 2019

Zusammenfassung
Hintergrund Multimorbidität stellt eine große Herausforderung in der Altersmedizin dar.
Methodik Darstellung explorativer Daten aus der Gerontopsychiatrie Studie Berlin (Gepsy-B), in der über einen Zeitraum von 3 Jahren Daten von 941 älteren stationären Patienten erfasst wurden.
Ergebnisse 94,2 % der Patienten litten an zumindest einer chronischen somatischen Erkrankung, im Mittel an 2,70 + 1,39, wobei die Anzahl mit dem Alter stieg (r = 0,257, p <,001). Die häufigsten waren zerebrovaskuläre (56,5 %) und Herzerkrankungen (52,2 %) sowie Hypertonus (54.8 %) und Diabetes mellitus Typ II (37,5 %). Zusätzlich litten viele Patienten an schweren körperlichen Beeinträchtigungen wie Bewegungsstörungen (26,2 %), schwerer Hypakusis (16,5 %), Inkontinenz (15,1 %) und ausgeprägter Sehminderung (7,4 %). Organische Hirnschädigungen waren dabei besonders häufig mit chronischen somatischen Erkrankungen oder Beeinträchtigungen assoziiert.
Schlussfolgerung Ältere psychiatrisch erkrankte Patienten haben oft zugleich eine ausgeprägte somatische Multimorbidität, die den Behandlungserfolg wesentlich mit beeinflussen dürfte.
Successful treatment of a mucous membrane pemphigoid in a young dog
Authors: Udraite Vovk Laura , Klinger Christoph J. , Böhm Teresa M. S. A. , Gedon Natalie K. Y. , Bettenay Sonya V. , Mueller Ralf. S. 
Published: October 2019

AbstractMucous membrane pemphigoid was diagnosed in a 2.5-year-old male intact foxhound-beagle cross which was presented with an acute onset of non-pruritic, multifocal, slowly progressive erosive-ulcerative dermatitis predominantly affecting the nasal planum, eyelids and muzzle with multiple vesicles on the inner pinnae, oral mucosa and tongue. The diagnosis was based on clinical signs and histological examination of skin biopsies. The patient did not respond to immunosuppressive prednisolone therapy, but went into complete remission with oral doxycycline and niacinamide and stayed in remission on long-term exclusive niacinamide treatment.
Klinische Kardiotoxizität bei Hunden nach Doxorubicintherapie
Authors: Betz Daniela Simon 
Published: October 2019

Hallman BE, Hauck ML, Williams LE et al. Incidence and risk factors associated with development of clinical cardiotoxicity in dogs receiving doxorubicin. J Vet Intern Med 2019; 33: 783–791.
Doxorubicin ist ein wichtiges Zytostatikum in der Behandlung vieler Tumorerkrankungen beim Hund. Eine klinisch relevante, vor allem kumulative Kardiotoxizität ist als unerwünschte Wirkung dieser Substanz beim Menschen gut charakterisiert. Beim Hund sind doxorubicinassoziierte kardiale Effekte hingegen nur wenig untersucht. Ziel dieser Studie war, die Inzidenz klinischer Kardiotoxizität nach Doxorubicinbehandlung beim Hund zu erheben sowie Risikofaktoren für deren Entwicklung zu identifizieren.
Mechanical Performance of a Polyaxial Locking Plate and the Influence of Screw Angulation in a Fracture Gap Model
Authors: Kaczmarek Jakub , Bartkowiak Tomasz , Schuenemann Riccarda , Paczos Piotr , Gapinski Bartosz , Bogisch Sandra , Unger Martin 
Published: October 2019

Abstract
Objective The aim of this study was to compare the locking compression plate (LCP) with polyaxial locking system (PLS) using single cycle to failure 4-point bending test and to investigate the behaviour of PLS with screws inserted mono- and polyaxially using cyclic fatigue test in two bending directions.
Materials and Methods Tests were performed on bone surrogates in a fracture gap model. The 3.5 LCP and 3.5 PLS plates were tested in single cycle to failure. The 3.5 PLS plates with mono- and polyaxial screws were compared in a cyclic fatigue tests in two orthogonal directions. For both experiments, micro-computed tomography (CT) scans were performed pre- and post-testing to investigate the connections between the screw head and the plate hole. Means of forces and cycles needed to failure were statistically compared.
Results The PLS plates were on average 30% weaker than LCP plates. Mode of failure was plate bending in the single cycle to failure tests, and plate breakage in the cyclic fatigue tests. Neither screw breakage nor loss of the screw–plate interface occurred. Mono- and polyaxial constructs performed similarly when loaded in the same direction. Micro-CT revealed no additional internal cracks in the plates or screws after testing. It also showed for both PLS and LCP that there was only partial contact of the screw head with the plate hole.
Clinical Relevance PLS offers a durable locking system, even when the screws are placed polyaxially. The weaker bending properties of the PLS compared with LCP should be considered during preoperative planning.
Uncus Herniation Presenting as Immediate Onset Postoperative Hemichorea
Authors: Agarwal Vinay , Singhal Namit 
Published: October 2019

AbstractChorea is defined as a state of excessive spontaneous movements, irregularly timed, nonrepetitive, randomly distributed, and abrupt in character. Thalamic and subthalamic lesions are commonly known to cause hemichorea. Here, we describe a patient with hydrocephalus due to aqueductal stenosis that caused hemichorea in the immediate postoperative period due to subthalamic infarct of uncus herniation. Considering that hydrocephalus symmetrically affects the central nervous system, the asymmetric presentation of chorea in the immediate postoperative period after wakening up from anesthesia could be confusing if a possibility of uncus herniation is not considered.
Acute Motor Axonal Neuropathy in a 5-Month-Old Child
Authors: Sullo Federica , Motta Milena , Smilari Pierluigi , Rampello Luigi , Greco Filippo , Pavone Piero 
Published: October 2019

AbstractGuillain–Barré syndrome (GBS) is an acute inflammatory polyneuropathy characterized by rapidly progressive, essentially symmetric weakness and areflexia in a previously otherwise healthy child. It is the most common cause of acute flaccid paralysis in children, and its reported incidence is 1 to 2/100,000 population. Prior infection is a well-established predating event in GBS. The commonly recognized variants of GBS are acute inflammatory demyelinating polyneuropathy (AIDP), acute motor axonal neuropathy (AMAN), acute motor sensory axonal neuropathy, and Miller–Fisher syndrome. AIDP is the most prevalent form. As Guillain–Barrè syndrome represents an important differential diagnosis in infancy with pronounced and progressive hypotonia, we herein report a case of AMAN in a 5-month-old male infant without known exposure to immunomodulating factors or infections.
Role of the Interventional Radiologist in Pancreatic Whole Organ and Islet Cell Transplantation
Authors: Shah Ketan Y. , Simpson Russell O. , Wang Yifan , Okoye Obi T. , Pandhi Mithil B. , Desai Shamit S. , Almario Alvarez Jorge A. 
Published: October 2019

AbstractPancreas transplantation is an exciting therapy which has been used for several decades in the treatment of diabetes mellitus. It can be performed as either a whole organ or islet cell transplant. The role of interventional radiologists in the management of whole organ transplants is evolving and includes treatment of postoperative complications and graft biopsy to evaluate for rejection. An in-depth understanding of the transplant anatomy and variations is a fundamental tool in performing these interventions successfully. Islet cell transplantation entails delivery of purified donor pancreatic islet cells into the recipient portal vein. Because of their expertise in image-guided access to the portal system, interventional radiologists play a crucial role in this procedure. The purpose of this article is to review the indications, anatomy, complications, and outcomes of both whole organ and islet cell pancreas transplants, followed by a discussion of the role of interventional radiologists in each procedure.
Binding of cloned Cel enzymes on clay minerals related to the pI of the enzymes and database survey of cellulases of soil bacteria for pI
Authors: Math Renukaradhya K , Kambiranda Devaiah , Yun Han Dae , Ghebreiyessus Yemane 
Published: October 2019

ABSTRACTThe Cel genes from Bacillus licheniformis MSB03 were cloned and expressed to investigate binding ability on clay minerals and sea sand at pH ranging 3 to 9. FTIR analysis has been done to characterize bound enzymes on clay minerals. Subsequent, surveying of NCBI database for extracellular enzymes of soil bacteria was carried out. Among the five cloned Cel enzymes assayed for binding to clay minerals, only Cel5H enzyme had the binding ability. Enzyme Cel5H exhibited highest binding to montmorillonite followed by kaolinite and sea sand. Interestingly, Cel5H had higher pI value of 9.24 than other proteins (5.2–5.7). Cel5H binding to montmorillonite was shown to be negatively affected below pH 3 and above pH 9. Infrared absorption spectra of the Cel5H-montmorillonite complexes showed distinct peaks for clay minerals and bound proteins. Furthermore, database survey of soil bacterial extracellular enzymes revealed that Bacillus species enzymes had higher pI than other soil bacterial enzymes.
Risk experience, emotions, place identity, and coping strategies in people affected by an unexpected fire (Experiencia de riesgo, emociones, identidad de lugar y estrategias de afrontamiento en personas afectadas por un incendio inesperado)
Authors: Bernardo Fátima , Santos Luís , Dias Débora , Rodrigues Mariana 
Published: October 2019

This study is intended to elucidate the impact of the experience of risk on emotions and coping strategies following an unexpected fire. It looked at residents of the Island of Madeira, where a highly destructive fire had recently occurred. Two groups were compared (people who had been affected directly by the fire event and others who had not) in order to study their risk perception, emotions and coping strategies, and to explore the variables that might contribute to explaining each group’s coping strategies. The results showed that people affected directly by the fire reported higher levels of active coping strategies, such as analysis and prevention. Women displayed higher levels of fear and less use of active coping strategies. The results also revealed that anger, local identity and fire experience predicted both analysis and prevention forms of coping. However, coping delegation was only predicted by local identity, and coping denial just by fear.
Fifth-Order Alice-Bob Systems and Their Abundant Periodic and Solitary Wave Solutions*
Authors: Zhao Qi-Liang , Jia Man , Lou Sen-Yue 
Published: October 2019

Abstract
The study on the nonlocal systems is one of the hot topics in nonlinear science. In this paper, the well-known fifth-order integrable systems including the Sawada-Kotera (SK) equation, the Kaup-Kupershmidt (KK) equation and the fifth-order Koterweg-de Vrise (FOKdV) equation are extended to a generalized two-place nonlocal form, the generalised fifth-order Alice-Bob system. The Lax integrability of two sets of Alice-Bob systems for all the SK, KK and FOKdV type systems are explicitly given via matrix Lax pairs. The

P
^

T
^

symmetry breaking and symmetry invariant periodic and solitary waves for one set of nonlocal SK, KK and FOKdV system are investigated via a special travelling wave solution ansatz.
Magnetohydrodynamic Electroosmotic Flow with Patterned Charged Surface and Modulated Wettability in a Parallel Plate Microchannel*
Authors: Hao Na , Jian Yong-Jun 
Published: October 2019

Abstract
This paper investigates the magnetohydrodynamic (MHD) electroosmotic flow (EOF) of Newtonian fluid through a zeta potential modulated parallel plate microchannel with patterned hydrodynamic slippage. The driven mechanism of the flow originates from the Lorentz force generated by the interaction of externally imposed lateral electric field Ey
and vertical magnetic field Bz
and electric field force produced by an externally applied electric field Ex
. It is assumed that the wall zeta potential and the slip length are periodic functions of axial coordinate x, an analytical solution of the stream function is achieved by utilizing the method of separation of variables and perturbation expansion. The pictures of streamlines are plotted and the vortex configurations produced in flow field due to patterned wall potential and hydrodynamic slippage are discussed. Based on the stream function, the velocity field and volume flow rate are obtained, which are greatly depend on some dimensionless parameters, such as slip length ls
, electrokinetic width λ, the amplitude δ of the patterned slip length, the amplitude m of the modulated zeta potential and Hartmann number Ha. The variations of velocity and volume flow rate with these dimensionless parameters are discussed in details. These theoretical results may provide some guidance effectively operating micropump in practical nanofluidic applications.
Gravitational Deflection of Massive Particles by a Schwarzschild Black Hole in Radiation Gauge*
Authors: Li Zong-Hai , Zhou Xia , Li Wei-Jun , He Guan-Sheng 
Published: October 2019

Abstract

The exact metric of a Schwarzschild black hole in the true radiation gauge was recently reported. In this work, we base on this gravity and calculate the gravitational deflection of relativistic massive particles up to the fourth post-Minkowskian order. It is found that the result is consistent with the previous formulations for both the case of dropping the fourth-order contribution and the case of light deflection. Our result might be helpful for future high-accuracy observations.
Coupling Instability of a Warm Relativistic Electron Beam with Ion-Channel Guiding
Authors: Rostami A. , Hajisharifi K. , Mehdian H. , Hasanbeigi A. 
Published: October 2019

Abstract

In this paper, the coupling instability of warm relativistic electron beam (WREB) propagating through the ion channel guiding is investigated in detail. Obtaining the equilibrium state of the system by considering the self-electric and azimuthal magnetic field, the fluid-Maxwell equations as well as linear perturbation theory are employed to derive the dispersion relation of the excited modes in the system. Numerical analysis of the obtained dispersion relation shows that the electromagnetic (EM) instability can be induced nearly the center of the beam through coupling between the fast electron plasma wave (FEPW), originated from the longitudinal oscillation of WREB, and fast forward electromagnetic wave (FFEW). In this sense, growing the perturbation amplitude occurs due to transport the kinetic energy of WREB to the EM wave at the specific frequency range, where the phase velocity of FEPW and FFEW is coincided. The results of the present investigation will greatly contribute to the understanding of the stability of the warm relativistic electron beam in laboratory experiments, such as in free electron laser experiments, where the ion-channel guiding is used to confine the electrons against the self-repulsive forces generated by the beam itself.
Magnetic Properties of an Antiferromagnetic Spin-1/2 XYZ Model in the Presence of Different Magnetic Fields: Finite-Size Effects of Inhomogeneity Property*
Authors: Arian Zad Hamid , Zoshki Azam , Sabeti Moones 
Published: October 2019

Abstract

Magnetic and thermodynamic properties of the anisotropic XYZ spin-1/2 finite chain under both homogeneous and inhomogeneous magnetic fields are theoretically studied at low temperature. Using exact diagonalization method (ED), we study the magnetization, magnetic susceptibility, and specific heat of the model characterized in terms of the finite correlation length in the presence of three different magnetic fields including longitudinal, transverse, and transverse staggered magnetic fields. The magnetization, susceptibility, and the specific heat of the model are investigated under two conditions separately: (i) When the model is putted in the presence of homogeneous magnetic fields. (ii) When finite inhomogeneities are considered for all applied magnetic fields in the Hamiltonian. We show that for the finite-size XYZ chains at low temperature, the evident magnetization plateaus gradually convert to their counterpart quasi-plateaus when the transverse magnetic field increases. Moreover, the influence of the transverse and staggered transverse magnetic fields, and their corresponding inhomogeneities on the magnetization process, magnetic susceptibility, and specific heat are reported in detail. Our exact results illustrate that by altering the inhomogeneity parameters, magnetization plateaus gradually convert to their counterpart quasi-plateaus. The specific heat manifests Schottky-type maximum, double-peak, and triple-peak, as well as, transformation between them by varying considered inhomogeneity parameters in the Hamiltonian.
Effect of toroidal plasma currents on the Wendelstein 7-X Scrape-Off Layer
Authors: Killer Carsten , Gao Yu , Perseo Valeria , Rudischhauser Lukas , Hammond Kenneth , Buttenschön Birger , Barbui Tullio , Blackwell Boyd D , Brunner Kai-Jakob , Drews Philipp , Endler Michael , Geiger Joachim , Grulke Olaf , Jakubowski Marcin , Klose Sören , Knauer Jens , Knieps Alexander , König Ralf , Li Yongliang , Neuner Ulrich , Niemann Holger , Otte Matthias , Schilling Jonathan , Sitjes Aleix Puig , Rahbarnia Kian , Stange Torsten 
Published: October 2019

Abstract
The role of toroidal plasma currents for the island divertor scrape-off layer in the stellarator Wendelstein 7-X is investigated using reciprocating electric probes. Experiments show that small amounts (of a few kA) of plasma current are sufficient to significantly affect the scrape-off layer plasma conditions, whereas higher plasma currents above 10kA result in more drastic changes. This behavior is linked to the effect of the plasma current on the rotational transform profile, which can result in significant shifts of the edge magnetic islands. These shifts affect the interaction of the islands with the divertor and can eventually result in a transition from a diverted to a limited plasma configuration. The probe observations are complemented by further edge diagnostics including plasma flow measurements, divertor Langmuir probes, divertor thermography and impurity spectroscopy.
Dynamical creation and detection of entangled many-body states in a chiral atom chain
Authors: Buonaiuto Giuseppe , Jones Ryan , Olmos Beatriz , Lesanovsky Igor 
Published: October 2019

Abstract
Open quantum systems with chiral interactions can be realized by coupling atoms to guided radiation modes in photonic waveguides or optical fibers. In their steady state these systems can feature intricate many-body phases such as entangled dark states, but their detection and characterization remains a challenge. Here we show how such collective phenomena can be uncovered through monitoring the record of photons emitted into the guided modes. This permits the identification of dark entangled states but furthermore offers novel capabilities for probing complex dynamical behavior, such as the coexistence of a dark entangled and a mixed phase. Our results are of direct relevance for current optical experiments, as they provide a framework for probing, characterizing and classifying classical and quantum dynamical features of chiral light–matter systems.