bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resolvin E1 accelerates pulp repair by regulating inflammation and stimulating dentin regeneration in dental pulp stem cells
Authors: Chen Jie , Xu Huaxing , Xia Kun , Cheng Shuhua , Zhang Qi 
Published: October 2020

Abstract
Background: Unresolved inflammation and tissue destruction are considered to underlie failure of dental pulp repair. As key mediators of the injury response, dental pulp stem cells (DPSCs) play a critical role in pulp tissue repair and regeneration. Resolvin E1 (RvE1), a major dietary omega-3 polyunsaturated fatty-acid metabolite, is effective in resolving inflammation and activating wound healing. However, whether RvE1 facilitates injured pulp-tissue repair and regeneration through timely resolution of inflammation and rapid mobilization of DPSCs is unknown. Therefore, we established a pulp injury model and investigated the effects of RvE1 on DPSC-mediated inflammation resolution and injured pulp repair.Methods: A pulp injury model was established using eight-week-old Sprague-Dawley rats. Animals were sacrificed on days 1, 3, 7, 14, 21, and 28 after pulp capping with a collagen sponge immersed in PBS with RvE1 or PBS. Hematoxylin-eosin and Masson’s trichrome staining, immunohistochemistry, and immunohistofluorescence were used to evaluate the prohealing properties of RvE1. hDPSCs were incubated with lipopolysaccharide (LPS) to induce an inflammatory response, and the expression of inflammatory factors after RvE1 application was measured. Effects of RvE1 on hDPSC proliferation, chemotaxis, and odontogenic differentiation were evaluated by CCK-8 assay, transwell assay, alkaline phosphatase (ALP) staining, alizarin red staining, and quantitative PCR, and possible signaling pathways were explored using western blotting.Results: In vivo, RvE1 promoted injured pulp repair and reparative dentin formation. Further, it enhanced dentin sialoprotein expression and accelerated pulp inflammation resolution by suppressing TNF-α and IL-1β expression. RvE1 enhanced the recruitment of CD146+ and CD105+ DPSCs to the damaged molar pulp mesenchyme. Isolated primary cells exhibited the mesenchymal stem cell immunophenotype and differentiation. RvE1 promoted hDPSC proliferation and chemotaxis. RvE1 significantly attenuated pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) release and enhanced ALP activity, nodule mineralization, and especially, expression of the odontogenesis-related genes DMP-1, DSPP, and BSP in LPS-stimulated DPSCs. RvE1 regulated Akt, ERK, and rS6 phosphorylation in LPS-stimulated DPSCs.Conclusions: RvE1 promotes pulp inflammation resolution and dentin regeneration and positively influences the proliferation, chemotaxis, and differentiation of LPS-stimulated hDPSCs. This response is, at least partially, dependent on Akt, ERK, and rS6-associated signaling in the inflammatory microenvironment. RvE1 has promising application potential in regenerative endodontics.
LRIG1 expression and colorectal cancer prognosis
Authors: Bakherad Maryam , Salimi Mahdieh , Angaji Seyed Abdolhamid , Mahjoubi Frouzandeh , Majidizadeh Tayebeh 
Published: October 2020

Abstract
Background

Make the right treatment decisions about colorectal cancer (CRC) patients need reliable predictive and prognostic data. However, in many cases this data is not enough. Some studies suggest that LRIG1 gene (leucine-rich repeats and immunoglobulin-like domains1) has prognostic implications in different kinds of cancers.
Methods

One hundred and two patients with colorectal cancer were retrospectively analyzed for LRIG1 expression at both mRNA and protein levels. SYBR Green Real-Time RT-PCR technique was used for mRNA expression analyses and Glyceraldehyde-3-Phosphate Dehydrogenase gene (GAPDH) was considered as a reference gene for data normalization. LRIG1 protein expression was analyzed using IHC. Additionally, appropriate statistical analyses were used to assess the expression of LRIG1 in test and control groups. The prognostic significance of LRIG1 expression was analyzed using the univariate and multivariate analyses.
Results

The data revealed the expression of LRIG1 in both mRNA and protein levels was dawn regulated in colorectal tumor tissues (P < 0.01) but is not clinically relevant prognostic indicator in CRC.
Conclusions

Therefore, it is suggested that LRIG1 expression analyses may not be considered as an important issue when making informed and individualized clinical decisions regarding the management of colorectal cancer patients.
Accessing the syndemic of COVID-19 and malaria intervention in Africa
Authors: Shi Benyun , Zheng Jinxin , Xia Shang , Lin Shan , Wang Xinyi , Liu Yang , Zhou Xiao-Nong , Liu Jiming 
Published: October 2020

Abstract
Background: The COVID-19 pandemic has caused substantial disruptions to health services in the low and middle-income countries with a high burden of other diseases, such as malaria in sub-Saharan Africa. As the COVID-19 pandemic spread to Africa, there is an urgent need to assess the impact of COVID-19 pandemic on malaria transmission potential in malaria-endemic countries in Africa. Methods: We present a data-driven method to quantify the extent to which the COVID-19 pandemic, as well as various non-pharmaceutical interventions (NPIs), could lead to the change of malaria transmission potential in 2020. First, we adopt a particle Markov Chain Monte Carlo method to estimate epidemiological parameters in each country by fitting the time series of the cumulative number of reported COVID-19 cases. Then, we simulate the epidemic dynamics of COVID-19 under two groups of NPIs: (i) contact restriction and social distancing, and (ii) early identification and isolation of cases. Based on the simulated epidemic curves, we quantify the impact of COVID-19 epidemic and NPIs on the distribution of insecticide-treated nets (ITNs). Finally, by treating the total number of ITNs available in each country in 2020, we evaluate the negative effects of COVID-19 pandemic on malaria transmission potential based on the notion of vectorial capacity. Results: In this paper, we conduct case studies in four malaria-endemic countries, Ethiopia, Nigeria, Tanzania, and Zambia, in Africa. The epidemiological parameters (i.e., the basic reproduction number R_0 and the duration of infection DI) of COVID-19 in each country are estimated as follows: Ethiopia (R0=1.57, DI=5.32), Nigeria (R0=2.18, DI=6.58), Tanzania (R0=2.47, DI=6.01), and Zambia (R0=2.12, DI=6.96). Based on the estimated epidemiological parameters, the epidemic curves simulated under various NPIs indicated that the earlier the interventions are implemented, the better the epidemic is controlled. Moreover, the effect of combined NPIs is better than contact restriction and social distancing only. By treating the total number of ITNs available in each country in 2020 as a baseline, our results show that even with stringent NPIs, malaria transmission potential will remain higher than expected in the second half of 2020. Conclusions: By quantifying the impact of various NPI response to the COVID-19 pandemic on malaria transmission potential, this study provides a way to jointly address the syndemic between COVID-19 and malaria in malaria-endemic countries in Africa. The results suggest that the early intervention of COVID-19 can effectively reduce the scale of the epidemic and mitigate its impact on malaria transmission potential.
A High Level of lncFGD5-AS1 Inhibits Epithelial-to-Mesenchymal Transition by Regulating the miR-196a-5p/SMAD6/BMP axis
Authors: Liu Lin , Zhang Cheng , Wang Jizhao , Liu Xu , Qu Hangying , Zhang Guangjian , Liang Ting , Wang Jiansheng , Zhang Jia 
Published: October 2020

Abstract
BackgroundLong non-coding RNA (lncRNA) was a vital factor in the progression and initiation of human cancers. This study found a new lncRNA, FGD5-AS1, which can inhibit EMT process, proliferation, and metastasis in vitro and in vivo.MethodsqRT-PCR was employed to test the expression of lncFGD5-AS1 in 30 gastric cancer patients’ cancer tissue and para-cancer tissue. Overexpressed lncFGD5-AS1 cells shown sharply decrease of proliferation, migration, and epithelial-mesenchymal transition (EMT). miR-196a-5p/SMAD6 was confirmed as downstream molecular mechanism of lncFGD5-AS1 by expression correlation analysis and mechanism experiments. In vivo study illustrated overexpression of lncFGD5-AS1 suppression tumor growth.ResultsLncFGD5-AS1 served as a ceRNA of miR-196a-5p to release its inhibition on SMAD6, a conventional inhibitor on the BMP pathway. Comparing with normal gastric cancer cells, FGD5-AS1 overexpressed group had fewer migration cells, lower cell viability, and lower EMT transformation rate. Meanwhile, xenografts nude mice injecting with overexpressed-FGD5-AS1 cells also shown smaller tumor weight and volume. ConclusionIn conclusion, this research supported the first evidence that FGD5-AS1 suppressed proliferation and metastasis in gastric cancer by regulating miR-196a-5p/SMAD6/BMP axis and suggested a potential therapeutic candidate for gastric cancer.
A Molecular Survey and Genetic Diversity of Hepatitis E Virus in Domestic Swine From Slovakia
Authors: Jackova Anna , Dudasova Katarina , Salamunova Slavomira , Mandelik Rene , Novotny Jaroslav , Vilcek Stefan 
Published: October 2020

Abstract
Background: Hepatitis E virus (HEV) is agent causing hepatitis worldwide. Originally considered to be limited to developing countries, this virus was also detected in developed countries. In recent years an increasing number of reports indicate that farmed domestic pigs are widely infected with HEV in several European countries including neighbouring countries the Czech Republic and Hungary. The HEV status in Slovakia is still missing.Results: In this study, the circulation of HEV among domestic swine in Slovakia and genetic diversity of the virus was studied. Overall HEV RNA was detected in 53/388 (13.7%) pig rectal swabs in five production stages (age categories) with statistically highly significant differences (P < 0.01, χ2=28.444). No HEV was detected in suckling piglets and sows. The highest HEV incidence (29.6%) was observed in weaners and then declined in growers and fatteners. Twenty-eight partial sequences of ORF1 (242 bp) and seventeen of ORF2 (304 bp) were genetically analysed. Phylogenetic analysis and p-distance comparisons confirmed in both ORFs that all Slovak HEV sequences belong to the genotype HEV-3, major clade 3abchij with higher identity to 3a and 3i subtypes. Three sequences created a new branch, outside of major clades 3abchij and 3efg, showing high nucleotide p-distances (0.140-0.178). They could represent a novel subtype.Conclusion: This is the first report to fill the epidemiological gap about HEV infection in pigs in Slovakia. The results indicated a lower incidence of HEV in Slovak pig farms than observed in other European countries. While most HEV isolates were typed as HEV-3 clade3abchij, three sequences were most probably a candidate for a new HEV-3 subtype.
Establishment and validation of novel clinical prognosis nomograms for luminal A breast cancer patients with bone metastasis
Authors: Tu QiHao , Hu Chuan , Zhang Hao , Peng Chen , Kong Meng , Song MengXiong , Zhao Chong , Wang YuJue , Li Jianyi , Zhou ChuanLi , Wang Chao , Ma XueXiao 
Published: October 2020

Abstract
Purpose

Overall survival (OS) and cancer-specific survival (CSS) of luminal A breast cancer (BC) patients with bone metastasis remain poor and vary dramatically from person to person. Our goal was to build two universally applicable nomograms to accurately predict OS and CSS for luminal A patients with bone metastasis.
Methods

The data of lumial A BC patients with bone metastases between 2010 and 2015 were collected from the Surveillance, Epidemiology, and End Results (SEER) database collected from the Surveillance, Epidemiology, and End Results (SEER) database for luminal A BC patients with bone metastasis between 2010 and 2015. Univariate and multivariate Cox regression analyses were to assess and identify independent risk factors of OS and CSS. Integrating all significant predictors, nomograms and risk groups stratification model were developed. The performance of the nomogram was validated with concordance index (C-index), calibration plots, and decision curve analyses (DCA) for discriminative ability, calibration and clinical utility, respectively.
Results

3171 luminal A BC patients with bone metastasis were included. Through univariate and multivariate Cox regression analyses, 12 variables were identified as both independent OS- and CSS-related factors, including age, race, primary site, histology grade, tumor size, surgery, brain metastasis, liver metastasis, lung metastasis, estrogen receptor status, progesterone receptor status and insurance. Our nomograms for 1-, 3- and 5-year survival were based on those significant prognostic factors to develop. The C-indexes of OS- and CSS-nomograms in the training cohort were 0.701 and 0.704, respectively. Similar results were obtained in the validation cohort. The calibration curves and DCA presented satisfactory calibration and clinical utility.
Conclusion

Two nomograms have good discrimination, calibration and clinical utility, can accurately and effectively predict the prognosis of patients, and may benefit for clinical decision-making. In high-risk patients, more aggressive therapy and closer surveillance should be considered.
Transcriptome and Metabolome Analyses Reveal Key Genes potentially relative to sucrose and citrate accumulation in wampee (Clausena Lansium&nbsp;Skeels) Fruit During Fruit Advancement and Ripening
Authors: Peng Cheng , Chang Xiaoxiao , Chen Zhe , Qiu Jishui , Lin Zhixiong , Lu Yusheng 
Published: October 2020

Abstract
Background: The seedless Clausena lansium Skeels, belonged to the Rutaceae Clausena family, is the main commercial variety in China currently. The high citrate contents in mature fruit have seriously restricted the development of the industry. The dynamic changes of sucrose and citrate, as well as the involved key regulatory genes or metabolism pathways during the fruit development of seedless wampee, however, remain unclear. Results: In this study, we used the seedless wampee fruits at various advancement levels as materials and specified a total of 623 compounds by widely targeted metabolome. Metabolome analysis further revealed that sucrose was gradually accumulated throughout the fruit development, while citrate was accumulated rapidly in the transitionary period from fruit expansion to color conversion, and decreased slowly after color conversion period without significant difference. Moreover, we identified several key differentials expressed genes existed in sucrose as well as citrate metabolism, that mainly encode insoluble acid invertases (IVR), sucrose-phosphate synthase (SPSs), sucrose synthase (SuSy), NAD-dependent malate dehydrogenase (NAD-MDH), trehalose 6-phosphate synthase (TPS), and ATP citrate lyase (ACL), by comparing the transcriptome data of fruit in each stage. Conclusions: We found that sucrose content was mainly regulated by SUS, IVR, SPS, and TPS, the citrate synthesis was mainly regulated by MDH, and the decomposition of citrate was mainly controlled by ACLβ in the acetyl-CoA pathway, suggesting that GABA shunt pathway did not play a decisive role.
Bioelectrochemical Sysytem: An Eco-Friendly Approach To Generate Electricity Utilizing Plants And Microorganisms
Authors: DAVE KRUTI , Darji Parth , Gandhi Fenie , Singh Saumya , Jadav Digvijaysinh 
Published: October 2020

Abstract
With the expanding population, there is increase in an energy demand, which leads to the depletion of non-sustainable energy sources, for example; fossils. As the current situation speaks, the oil deposits are left for mere 53 years, likely with gas i.e. 52 years and with coal 150 years. So, there is an urgent need to find a sustainable source which is cheap and environment friendly, owing to the fact of current energy consumption level is left for merely 230 years. As committed by green alternative, for the future enhancement of the planet, the fossil fuel abandonment is required, and instigation of renewable resources such as Microbial Fuel Cell [MFCs] and Plant Microbial Fuel Cell [PMFCs] should be implemented. MFC is a visionary technique, as it converts wastage into the energy, whereas, PMFC is a new-fangled technique devoid of any climatic conditions and also it requires less investment. By scrutinizing this technique, Bacillus megaterium and sewage material is used in MFCs whereas Azolla and Trigonella foenum is used in PMFCs, which converts chemical energy into electrical energy with the help of electrons flowing from anode to cathode via circuit. The individual setup of each MFCs and PMFCs are examined diurnally for voltage and current gain proceeded by connection of both [MFC and PMFC] in series with LED in between thus gaining the luminance in LED. So assurance is gained by this technique of MFC and PMFC as distinctive energy harvesting technology equipped with; consistency, maintenance and free power for distant future.
High Detection Rate and Risk Factors of Melanosis Coli in End-Stage Renal Disease Patients Awaiting Kidney Transplantation: A Single-Center Retrospective Study in China
Authors: Zhang Wu-Xing , Zhou Wei , Li Yang , Wang Yang , Huang Wei 
Published: October 2020

Abstract
Background. Rhubarb-containing Traditional Chinese Medicine (TCM) is widely used to manage chronic renal failure in China. Studies show that anthranoids contained in rhubarb are related to the occurrence of melanosis coli (MC). However, no study on the detection rate of MC in end-stage renal disease (ESRD) patients in China has been reported.Methods. We conducted a retrospective study on 99 ESRD patients who received a colonoscopy as part of pre-transplant work-up between Jan 1, 2017 and July 1, 2020 in a single center in Beijing, China. 200 age- and gender-matched subjects with normal renal function and received a colonoscopy for health screening in the same period were randomly selected as control group. The detection rate of MC in ESRD patients and control subjects was calculated and compared. Univariate analysis and multivariate logistic regression were performed in succession to determine the association between ESRD and MC, and also probe the independent MC risk factors in ESRD patients.Results. Among the 99 ESRD patients, MC was detected in 19, with a detection rate of 19.2% (95% confidence interval [CI]: 11.3%, 27.1%). In contrast, among 200 control subjects with normal renal function, MC was only detected in 1 subject, with an detection rate of 0.5% (95%CI: 0.5%, 1.5%) (ESRD vs. control: odds ratio [OR] = 47.263, 95% CI: 6.222, 358.981; p = 0.000). After adjustment of age, body mass index(BMI), albumin, hemoglobin and Charlson comorbidity index, being ESRD was still associated with the presence of MC (Odds ratio[95%CI]: 36.251[3.435, 382.559], p = 0.003). In ESRD patients, both univariate analysis (OR = 4.358, 95% CI: 1.329, 14.286; p = 0.012) and multivariate logistic regression (OR = 4.916, 95% CI: 1.462, 16.525, p = 0.010) found that past use of Rhubarb-containing TCM was the only independent risk factor of MC.Conclusion. The prevalence of MC in this cohort of ESRD patients was 19.2%, which was significantly higher than that of control with normal renal function. Past use of Rhubarb-containing traditional Chinese herbs was the only independent risk factor for the detection of MC in these ESRD patients. Our work highlights firstly that when prescribing Rhubarb-containing TCM to ESRD patients, the risk of MC development should be alerted, and also there is a need for arranging high quality, well-designed studies to examine the prevalence, etiology, impact, management and prognosis of MC in ESRD patients, especially those awaiting kidney transplantation.
How Do People Choose to Be Informed? A Survey of The Information Searched for in The Choice of Primary Care Provider in Sweden
Authors: Hoffstedt Caroline , Fredriksson Magnus , Winblad Ulrika 
Published: October 2020

Abstract
Background: To stimulate quality through choice of provider, patients need to seek and base their decisions on both relevant and reliable information describing providers’ clinical quality. The purpose of this study was first to investigate what types of information and information sources patients turned to in the active choice of primary care provider. Second, it investigated whether a sub-group of patients considered more likely to actively seek information, also sought more advanced information about the clinical quality of providers. Methods: Data collection was performed through a web-based survey to the general adult (18+) Swedish population, for a net sample of 3 150 respondents. Descriptive statistics were used to study what types of information and information sources respondents used prior to their choice. Multiple regression analysis was employed to examine predictors for seeking relevant and reliable information describing providers’ clinical quality.Results: Patients in active choice situations searched mainly for basic information, for instance, how to switch providers and their geographical location, and used a median of one information source. Information sources used were mainly dependent sources, such as providers themselves, and family and acquaintances. The sub-group of individuals more likely to seek information were not found to seek more advanced forms of information. Conclusions: Not even the patients considered most likely to seek information prior to their choice of primary care provider, searched for information deemed necessary to make well-informed choices. Thus, patients did not act according to the theoretical assumptions underlying the patient choice reforms, i.e., making informed choices based on clinical quality in order to promote the best providers over inferior ones. The results call for governments and health care authorities to actively assess and develop providers’ clinical quality by means other than patient choice.
Minimize the Extent and Morbidity of Axillary Dissection for Node-positive Breast Cancer Patients: Implementation of Axillary Lymph Node Dissection Based on Breast Lymphatics Level
Authors: Yuan Qianqian , Hou Jinxuan , He Yukun , Liao Yiqian , Zheng Lewei , Wu Gaosong 
Published: October 2020

Abstract
BackgroundBreast cancer-related lymphedema (BCRL) is associated with extensive axillary dissection. Axillary lymph node dissection (ALND) based on breast lymphatics level (BLL) was proposed to minimize the surgical extent for node-positive breast cancer patients.MethodsA total of 156 consecutive sentinel lymph node-positive (SLN+) or clinically node-positive (CN+) patients underwent sentinel lymph node biopsy (SLNB) with indocyanine green and methylene blue (MB). The SLNs were injected with 0.1 ml MB before removal, and a standard ALND was subsequently performed. The nodes adjacent to the blue-stained breast lymph nodes (ABLNs) were sent for pathological examination separately by resecting serial tissue every 0.5 cm away from the marginal blue-stained ABLNs. Then, a pilot study comparing ALND based on BLL and standard ALND was performed.ResultsBLL were successfully identified in 20 SLN+ (100%) and 134 CN+ (98.5%) patients. The median number of BLL was four, ranging from three to six. A horizontal line 1.0 cm away from the superior blue-stained BLN and a vertical line 1.0 cm away from the medial blue-stained BLN formed BLL II, III, and IV. All of the additional positive nodes were within 1.0 cm of the blue-stained ABLNs. The minimized axillary dissection should resect upwards from the lowest breast lymphatic level that contains the first confirmed negative blue-stained ABLNs. In the pilot study, no patient developed axillary recurrence.ConclusionThe ALND surgical procedure based on BLL could minimize the surgical extent for pathological node-positive breast cancer patients and potentially reduce the BCRL rate.Trial registrationChiCTR1800014247