bluuebunny/crossref_metadata_2025_split huggingface dataset:
Youth-Centered Maternity Care: A Binational Qualitative Comparison of the Experiences and Perspectives of Latina Adolescents and Healthcare Providers
Authors: Decker Martha J. , Pineda Noelle , Gutmann-Gonzalez Abigail , Brindis Claire D. 
Published: November 2020

Abstract
Background

Although there is growing recognition of the importance of person-centered maternity care, the needs and perspectives of pregnant adolescents are rarely considered. The purpose of this study was to compare the maternity care experiences of Mexican-origin adolescents in Guanajuato, Mexico and Fresno, California from both youth and healthcare provider perspectives.
Methods

Qualitative interviews and focus groups were conducted with a total of 89 respondents, including pregnant and parenting adolescents as well as providers between December 2016 and July 2017. Adolescents also completed a short demographic survey prior to participation. Transcripts in English and Spanish were coded and thematically analyzed using Dedoose software. Results were compared by location and between youth and providers.
Results

Four themes emerged regarding patient-provider interactions: the need for communication and clear explanations, respectful versus judgmental providers, engaging youth in decision-making, and a focus on the age of the youth and their partners. While youth had similar perspectives and priorities in both locations, youth in Mexico reported more negative healthcare experiences than youth in California. Perspectives varied between the youth and providers, with providers in both California and Mexico identifying several structural challenges in providing quality care to adolescents. In California, challenges to supporting immigrant Latina adolescents and their families included language and translation issues as well as barriers to care due to immigration status and documentation. In both locations, providers also mentioned high patient caseloads and their own concerns about the youth’s life choices.
Conclusion

Youth-centered care requires more effective and respectful patient-provider communication, where adolescents are engaged in their healthcare decision-making and delivery options. Changes in patient-provider interactions can help improve the maternity care experiences and outcomes of Latina adolescents. Healthcare systems and providers need to reconfigure their approaches to focus on the needs and priorities of adolescents.
Self-Sustaining Vortices in Protoplanetary Disks: Setting the Stage for Planetary System Formation
Authors: Regály Zsolt , Kadam Kundan , Dullemond Cornelis 
Published: November 2020

Abstract
The core accretion scenario of planet formation assumes that planetesimals and planetary embryos are formed during the primordial, gaseous phases of the protoplanetary disk. However, how the dust particles overcome the traditional growth barriers is not well understood. Recently proposed viscous ring-instability explains the concentric rings observed in protoplanetary disks by assuming that the dust grains can reduce the gas conductivity, which can weaken the magneto-rotational instability. We present an analysis of this model with the help of GPU-based numerical hydrodynamic simulations of coupled gas and dust in the thin-disk limit. During the evolution of the disk the dusty rings become Rossby unstable and break up into a cascade of small-scale vortices. The vortices form secularly stable dusty structures, where the rapid dust growth can occur via the streaming instability. The phenomenon of self-sustaining vortices is consistent with observational constraints of exoplanets and sets a favorable environment for planetary system formation.
Relationship Between Fatty Liver Change and Nutritional Status After Total Gastrectomy in Gastric Cancer Patients: A Retrospective Study
Authors: Nakamura Naohiko , Kinami Shinichi , Fujita Jun , Kaida Daisuke , Tomita Yasuto , Miyata Takashi , Miyashita Tomoharu , Fujita Hideto , Ueda Nobuhiko , Takamura Hiroyuki 
Published: November 2020

Abstract
Background: The relationship between chronological nutritional changes and development of fatty liver after total gastrectomy (TG) in gastric cancer (GC) patients is still unclear. This study aimed to evaluate relationship between development of fatty liver and chronological changes of nutritional parameters during 12 months after TG.Methods: We retrospectively analyzed medical records of 59 patients with GC who underwent TG at the Kanazawa Medical University Hospital between January 2009 and December 2017. We defined fatty liver change as a mean liver-to-spleen attenuation ratio (L/S ratio) of less than 1.2 in the computed tomography images at 12 months after TG and divided the patients into fatty liver (FL) and non-FL groups from the L/S ratio. We analyzed serum levels of total protein and albumin, and psoas muscle index (PMI) before TG and at 6 and 12 months after TG in the non-FL and FL groups. Results: Six patients showed an L/S ratio of less than 1.2 at 12 months after TG and were included into FL group. There was no significant difference between the groups in serum parameters, L/S ratio, and PMI before TG. In the FL group, the mean levels of total protein and albumin decreased after TG and were significant lower at 6 months, compared with the non-FL group. And then, these levels in the FL group recovered at 12 months. In contrast, the mean levels of total protein and albumin in the non-FL group did not decrease below the preoperative levels throughout the year after surgery. As with laboratory parameters, all patients in the FL group showed decrease of PMI at 6 months after TG. This proportion was significantly higher than that in the non-FL group (100% vs 40.8%, P = 0.006).Conclusions: We evaluated that the patients with fatty liver occurring after TG had significantly lower levels of serum nutritional parameters and skeletal muscle index at 6 months, not but 12 months, after TG.
Macrophage Plasticity is Rac Signalling and MMP9 Dependant
Authors: Travnickova Jana , Nhim Sandra , Abdellaoui Naoill , Djouad Farida , Nguyen-Chi Mai , Parmeggiani Andrea , Kissa Karima 
Published: November 2020

Abstract
In vitro, depending on extracellular matrix (ECM) architecture, macrophages migrate either in amoeboid or mesenchymal mode; while the first is a general trait of leukocytes, the latter is associated with tissue remodelling via Matrix Metalloproteinases (MMPs). To assess whether these stereotyped migrations could be also observed in a physiological context, we used the zebrafish embryo and monitored macrophage morphology, behaviour and capacity to mobilisation haematopoietic stem/progenitor cells (HSPCs), as a final functional readout. Morphometric analysis identified 4 different cell shapes. Live imaging revealed that macrophages successively adopt all four shapes as they migrate through ECM. Treatment with inhibitors of MMPs or Rac GTPase to abolish mesenchymal migration, suppresses both ECM degradation and HSPC mobilisation while differently affecting macrophage behaviour. This study depicts real time macrophage behaviour in a physiological context and reveals extreme reactivity of these cells constantly adapting and switching migratory shapes to achieve HSPCs proper mobilisation.
Organic Matter and Water from Asteroid Itokawa
Authors: Chan Queenie , Stephant Alice , Franchi Ian , Zhao Xuchao , Brunetto Rosario , Kebukawa Yoko , Noguchi Takaaki , Johnson Diane , Price Mark , Harriss Kathryn , Zolensky Michael , Grady Monica 
Published: November 2020

Abstract
Understanding the true nature of extra-terrestrial water and organic matter that was present at the birth of our solar system, and their subsequent evolution, necessitates the study of pristine astromaterials. In this study, we have studied both the water and organic contents from a dust particle recovered from the surface of near-Earth asteroid 25143 Itokawa by the Hayabusa mission, which represents one of the most uncontaminated astromaterial samples in Earth’s collection. The organic matter is presented as both nanocrystalline graphite and disordered polyaromatic carbon with high D/H and 15N/14N ratios (δD = +4,868 ± 2,288‰; δ15N = +344 ± 20‰) signifying an explicit extra-terrestrial origin. The contrasting organic feature (graphitic and disordered) substantiates the rubble-pile asteroid model of Itokawa, and offers support for material mixing in the asteroid belt that occurred in scales from small dust infall to cartographic impacts of large asteroidal parent bodies. Our analysis of Itokawa water indicates that the asteroid has incorporated D-poor water ice at the abundance on par with inner solar system bodies. The asteroid was metamorphosed and dehydrated on the formerly large asteroid, and was subsequently evolved via late-stage hydration, modified by D-enriched extraneous organics and water derived from a carbonaceous parent body.
EYS Mutations and Implementation of Minigene Assay for Variant Classification in EYS-Associated Retinitis Pigmentosa in Northern Sweden
Authors: Westin Ida Maria , Jonsson Frida , Österman Lennart , Holmberg Monica , Burstedt Marie , Golovleva Irina 
Published: November 2020

Abstract
Retinitis pigmentosa (RP) is a clinically and genetically heterogeneous group of inherited retinal degenerations. The ortholog of Drosophila eyes shut/spacemaker, EYS on chromosome 6q12 is a major genetic cause of recessive RP worldwide, with prevalence of 5 to 30%. In this study, by using targeted NGS, MLPA and Sanger sequencing we uncovered the EYS gene as the second most common genetic cause of RP in northern Sweden accounting for at least 18%. The most frequent pathogenic variant was c.8648_8655del that in some patients was identified in cis with c.1155T>A, indicating Finnish ancestry. We also showed that two novel EYS variants, c.2992_2992+6delinsTG and c.3877+1G>A caused exon skipping in human embryonic kidney cells, HEK293T and in retinal pigment epithelium cells, ARPE-19 demonstrating that in vitro minigene assay is a straightforward tool for the analysis of intronic variants. We conclude, that whenever it is possible, functional testing is of great value for classification of intronic EYS variants and the following molecular testing of family members, their genetic counselling, and inclusion of RP patients to future treatment studies.
Relationship Between Oxygen Radical Absorbance Capacity&nbsp;(ORAC) Index, Body Composition and Blood Biochemical Markers in Overweight /Obese Compared to Normal Weight Subjects: A Cross-Sectional Study
Authors: Madani Zahra , Javardi Maryam Moussavi , Khalili Ghazaleh , Djazayeri Sayed Abolghassem , Movahedi Ariyo , Karandish Majid 
Published: November 2020

Abstract
Background: Obesity is an important preventable disease, which promotes the development of chronic disorders by altering several factors including oxidative stress. Dietary antioxidants protect the body against oxidative stress. The purpose of this study was to evaluate the potential association of oxygen radical absorbance capacity (ORAC) index with obesity and its associated features in adults.Methods: participants were divided in two groups of normal weight and overweight /obesity. General characteristics were registered and a 147-item food frequency questionnaire was completed. Thereafter, anthropometric measurements were conducted. Biochemical indices were obtained from science and Research branch of Islamic Azad University of Tehran, Iran. The amount of the dietary ORAC index was estimated using the data provided by the USDA Foods Table.Results: The results showed that there was a significant difference between the normal and overweight/obese groups in terms of body mass index (BMI) and waist-to-hip ratio (WHR) (P=0.0001). Participants with normal weight consumed more fruits and vegetables than another group. Also, the dietary ORAC index in normal individuals was higher than the case group, but there was not significant difference (P=0.352). There was also a significant inverse correlation between dietary ORAC and BMI and WHR, whereas a significant positive correlation between dietary ORAC and plasma HDL was observed (P<0.05).Conclusion: Our findings suggest that dietary antioxidants is inversely associated with BMI, fasting blood glucose, triglyceride, total Cholesterol and LDL in both groups. It seems that following a diet rich in antioxidants can counteract obesity and its associated comorbidities.
The Protective Impacts of Spriulina Platensis Against Cisplatin-Induced Renal Injury Through The Regulation of Oxidative Stress, Pro-Inflammatory Cytokines and Bax/Bcl2 Expression Cascade
Authors: Abouzed Tarek , Soliman Mohamed , Khatab Shymaa , Elgazzar Ahmed , Gouda Wael , Dorghamm Doaa 
Published: November 2020

Abstract
One of the main antineoplastic chemotherapy medications is cisplatin; nephropathy is a major side effect of cisplatin. The current study investigates the molecular protective effect of Spirulina Platensis (SP) on cisplatin-induced nephrotoxicity. Forty eight healthy male albino rats were allocated into 4 groups. Group 1 received saline intraperitoneally (IP) twice per week (normal rats). Group 2, received SP (100 mg/kg bw orally). Group 3 injected cisplatin (1.5mg/kg IP) twice per week. Group 4 received SP and at 4th day received cisplatin (1.5mg/kg IP) for 21 days. After 3 weeks of experimentation, blood and renal tissues were taken for serum analysis, gene expression using qRT-PCR and renal histopathology. SP significantly ameliorated the alterations in the body weight, relative kidney weight, and the disturbance in examined renal markers. Furthermore oxidative stress biomarkers (MDA, NO, SOD, and GSH) induced by cisplatin were recovered and restored by SP. Cisplatin induced upregulation in the gene expression of TNF-α, iNOS, TGF1-β, IL-1β and IL-6 that were ameliorated by pre-administration of SP. Finally, cisplatin upregulated pro-apoptotic gene; Bax and downregulated anti-apoptotic gene; Bcl2. Of interest, SP mitigated this alteration in apoptosis and anti-apoptosis associated genes. Renal histopathology revealed the protective impacts of SP against cisplatin induced severe glomerular congestion, hemorrhage, inflammatory cell infiltration, degeneration and sever necrosis in renal glomeruli and tubules. In conclusion, SP has protective impact against cisplatin induced renal damage through the modulation of oxidative stress, anti-inflammatory, anti-necrotic and anti-apoptotic associated genes.
Layer Number Dependent Ferroelasticity in 2D Ruddlesden-Popper Organic-inorganic Hybrid Perovskites
Authors: Xiao Xun , Zhou Jian , Song Kepeng , Zhao Jingjing , Zhou Yu , Rudd Peter , Han Yu , Li Ju , Huang Jinsong 
Published: November 2020

Abstract
Ferroelasticity represents material domains possessing spontaneous strain that can be switched by external stress. Three-dimensional (3D) perovskites like methylammonium lead iodide (MAPI), which have demonstrated efficient solar cells and photodetectors, are determined to be ferroelastic. Apart from 3D perovskites, Ruddlesden-popper layered perovskites have been applied in optoelectronic devices with outstanding performance. However, the understanding of lattice strain as well as ferroelasticity in 2D layered perovskites is still lacking. Here，using the in-situ observation of switching domains in layered perovskite single crystals under external strain, we discover the existence of ferroelasticity in layered perovskites with layer number more than one, while the 2D perovskites with single octahedra layer do not show ferroelasticity. Density functional theory calculation shows that ferroelasticity in 2D perovskites originates from the distortion of inorganic octahedra resulting from the rotation of aspherical methylammonium cations. Additionally, the absence of methylammonium cations in single layer perovskite is consistent with the lack of ferroelasticity. These ferroelastic domains do not induce non-radiative recombination or reduce the photoluminescence quantum yield appreciably, indicating the glissile ferroelastic twin boundaries are benign defects. Our findings provide scientific insights in understanding strain-dependent material properties in 2D perovskites and lead to new potential flexible electronics controlled by strain engineering.
Differences in Antibiotic and Antiviral Use in People With Confirmed Influenza: a Retrospective Comparison of Rapid Influenza&nbsp;PCR and Multiplex Respiratory Virus PCR Tests.
Authors: Yeung Victor Au , Thapa Kiran , Rawlinson William , Georgiou Andrew , Post Jeffrey , Overton Kristen 
Published: November 2020

Abstract
BACKGROUND Influenza is a highly contagious respiratory virus with clinical impacts on patient morbidity, mortality and hospital bed management. The effect of rapid nucleic acid testing (RPCR) in comparison to standard multiplex PCR (MPCR) diagnosis in treatment decisions is unclear. This study aimed to determine whether RPCR influenza testing in comparison to standard MPCR testing was associated with differences in antibiotic and antiviral (oseltamivir) utilisation and hospital length of stay in emergency department and inpatient hospital settings. METHODS A retrospective cohort study of positive influenza RPCR and MPCR patients was performed utilising data from the 2017 influenza season. Medical records of correlating patient presentations were reviewed for data collection. An analysis of RPCR versus MPCR patient outcomes was performed examining test turnaround time, antibiotic initiation, oseltamivir initiation and hospital length of stay for both emergency department and inpatient hospital stay. Subgroup analysis was performed to assess oseltamivir use in high risk populations for influenza complications. Statistical significance was assessed using Mann-Whitney test for numerical data and Chi-squared test for categorical data. Odds ratio with 95% confidence intervals were calculated where appropriate.RESULTS Overall, 122 RPCR and 362 MPCR positive influenza patients were included in this study. Commencement of antibiotics was less frequent in the RPCR than MPCR cohorts (51% vs 67%; p <0.01, OR 0.52; 95% CI 0.34-0.79). People at high risk of complications from influenza who were tested with the RPCR were more likely to be treated with oseltamivir compared to those tested with the MPCR (76% vs 63%; p = 0.03, OR 1.81; 95% CI 1.07-3.08). Hospital length of stay was not impacted when either test was used in the emergency department and inpatient settings. CONCLUSIONS These findings suggest utilisation of RPCR testing in influenza management can improve antibiotic stewardship through reduction in antibiotic use and improvement in oseltamivir initiation in those at higher risk of complications. Further research is required to determine other factors that may have influenced hospital length of stay and a cost-benefit analysis should be undertaken to determine the financial impact of the RPCR test.
Microchannelled chitosan sponge grafting with hydrophobic alkyl chain for enhanced noncompressible hemorrhage and tissue regeneration
Authors: Du Xinchen , Wu Le , Jiang Zhuyan , Yan Hongyu , Li Shilin , Li Wen , Cheng Zhaojun , Bai Yanli , Wang Hongjun , Kong Deling , Zhu Mei Feng , Wang Lianyong 
Published: November 2020

Abstract
Developing an anti-infective shape-memory hemostatic sponge with ability of guiding in situ tissue regeneration for noncompressible hemorrhage in civilian and battlefield settings remains a challenge. Here, hemostatic chitosan sponge with highly interconnective microchannels was engineered by combining 3D printed fiber leaching and freeze-drying methods and then modified with hydrophobic alkyl chains. The microchannelled alkylated chitosan sponge (MACS) exhibited a strong capacity for water/blood absorption and rapid shape recovery. Compared to clinically used gauze, gelatin sponge, CELOX, and CELOX-gauze, the MACS demonstrated higher pro-coagulant and hemostatic capacities in lethally normal/heparinized rat and pig liver perforation models. Also, it exhibited strong anti-infective activity against S. aureus and E. coli. Additionally, it promoted liver parenchymal cell infiltration, vascularization, and tissue integration in a rat liver defect model. Overall, the MACS demonstrated promising clinical translational potential in cost effectively treating lethal noncompressible hemorrhage and in facilitating wound healing.
Wittig reagents for chemoselective sulfenic acid ligation enables global site stoichiometry analysis and redox-controlled mitochondrial targeting
Authors: Shi Yunlong , Fu Ling , Yang Jing , Carroll Kate 
Published: November 2020

Abstract
Triphenylphosphonium ylides, known as Wittig reagents, are one of the most commonly used tools in synthetic chemistry. Despite their considerable versatility, Wittig reagents have not yet been explored for their utility in biological applications. Here, we introduce a new chemoselective ligation reaction that harnesses the reactivity of Wittig reagents and the unique chemical properties of sulfenic acid, a pivotal post-translational cysteine modification in redox biology. The reaction, which generates a covalent bond between the ylide nucleophilic α-carbon and electrophilic γ-sulfur is highly selective, rapid, and affords robust labeling under a range of biocompatible reaction conditions, including in living cells. We highlight the broad utility of this conjugation method to enable site-specific proteome-wide stoichiometry analysis of S-sulfenylation, visualize redox-dependent changes in mitochondrial cysteine oxidation, and redox-triggered TPP generation for controlled delivery of small molecules to mitochondria.