bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detecting single ORAI1 proteins within the plasma membrane reveals higher-order channel complexes
Authors: Alansary Dalia , Peckys Diana B. , Niemeyer Barbara A. , de Jonge Niels 
Published: December 2019

ABSTRACT
ORAI1 proteins form highly selective Ca2+ channels in the plasma membrane. Crystallographic data point towards a hexameric stoichiometry of ORAI1 channels, whereas optical methods postulated ORAI1 channels to reside as dimers at rest, and other data suggests that they have a tetrameric configuration. Here, liquid-phase scanning transmission electron microscopy (STEM) and quantum dot (QD) labeling was utilized to study the conformation of ORAI1 proteins at rest. To address the question of whether ORAI1 was present as a dimer, experiments were designed using single ORAI1 monomers and covalently linked ORAI1 dimers with either one or two label-binding positions. The microscopic data was statistically analyzed via the pair correlation function. Label pairs were found in all cases, even for concatenated dimers with one label-binding position, which is only possible if a significant fraction of ORAI1 was assembled in larger order oligomers than dimers, binding at least two QDs. This interpretation of the data was consistent with Blue Native PAGE analysis showing that ORAI1 is mainly present as a complex of an apparent molecular mass larger than that calculated for a dimer.
Different effects of soybean isoflavone genistein on transforming growth factor levels during orthodontic tooth movement among young and old rabbits
Authors: Indriasari Verastuti , Suparwitri Sri , Christnawati Christnawati , Alhasyimi Ananto Ali 
Published: December 2019

Background: Orthodontic treatment to improve aesthetics and for health reasons is performed in children and adults. Elderly individuals have low levels of estrogen, this results in alveolar bone resorption being greater than alveolar bone apposition. Isoflavones present in soybeans may be able to improve the remodeling process through the induction of osteoblastogenesis by increasing transforming growth factor-β1 (TGF-β1) levels. This study aimed to assess the comparative effect of soybean genistein isoflavone to TGF-β1 during orthodontic tooth movement among juvenile and adult rabbits. Methods: In this study, 12 healthy female rabbits were used. Subjects were divided into four groups (n=3); YG group (young rabbits), YGI group (young rabbits + isoflavones genistein), OG group (old rabbits), and OGI group (old rabbits + isoflavones genistein). Two lower incisors of the rabbit were moved distally using an orthodontic force (50 grams force) delivered by an open coil spring, which was inserted between two brackets. During active movements, the genistein isoflavones were given from the initial installation of the device until days 21, at a dose of 1.2 mg/kg BW once a day. Measurement of TGF-β levels were performed on days 1, 7, 14, 21 after appliance installation. TGF-β1 expression was analyzed using enzyme-linked immunosorbent assay (ELISA) and the optical density (OD) of the sample quantifed using a standard curve. The data obtained were analyzed using one-way Anova followed by Tukey HSD test. Results: The TGF-β1 levels were found to highest in the YGI group, and the TGF-β levels were significantly lower in the OG group (p<0.05). ELISA analysis also revealed that TGF-β1 levels of the OGI group were significantly higher when compared with the OG group (p<0.05). Conclusion: The administration of soybean genistein isoflavones could improve TGF-β1 levels in old rabbit’s during active orthodontic tooth movement.
Functional group and diversity analysis of BIOFACQUIM: A Mexican natural product database
Authors: Sánchez-Cruz Norberto , Pilón-Jiménez B. Angélica , Medina-Franco José L. 
Published: December 2019

Background: Natural product databases are important in drug discovery and other research areas. Their structural contents and functional group analysis are relevant to increase their knowledge in terms of chemical diversity and chemical space coverage. BIOFACQUIM is an emerging database of natural products characterized and isolated in Mexico. Herein, we discuss the results of a first systematic functional group analysis and global diversity of an updated version of BIOFACQUIM. Methods: BIOFACQUIM was augmented through a literature search and data curation. A structural content analysis of the dataset was done. This involved a functional group analysis with a novel algorithm to identify automatically all functional groups in a molecule and an assessment of the global diversity using consensus diversity plots. To this end, BIOFACQUIM was compared to two major and large databases: ChEMBL 25, and a herein assembled collection of natural products with 169,839 unique compounds. Results: The structural content analysis showed that 16.1% of compounds, 11.3% of scaffolds, and 6.3% of functional groups present in the current version of BIOFACQUIM have not been reported in the other large reference datasets. It also gave a diversity increase in terms of scaffolds and molecular fingerprints regarding the previous version of the dataset, as well as a higher similarity to the assembled collection of natural products than to ChEMBL 25, in terms of diversity and frequent functional groups. Conclusions: A total of 148 natural products were added to BIOFACQUIM, which meant a diversity increase in terms of scaffolds and fingerprints. Regardless of its relatively small size, there are a significant number of compounds, scaffolds, and functional groups that are not present in the reference datasets, showing that curated databases of natural products, such as BIOFACQUIM, can serve as a starting point to increase the biologically relevant chemical space.
Widespread use of the “ascidian” mitochondrial genetic code in tunicates
Authors: Pichon Julien , Luscombe Nicholas M. , Plessy Charles 
Published: December 2019

Background:Ascidians, a tunicate class, use a mitochondrial genetic code that is distinct from vertebrates and other invertebrates. Though it has been used to translate the coding sequences from other tunicate species on a case-by-case basis, it is has not been investigated whether this can be done systematically. This is an important because a) some tunicate mitochondrial sequences are currently translated with the invertebrate code by repositories such as NCBI GenBank, and b) uncertainties about the genetic code to use can complicate or introduce errors in phylogenetic studies based on translated mitochondrial protein sequences.Methods:We collected publicly available nucleotide sequences for non-ascidian tunicates including appendicularians such as Oikopleura dioica, translated them using the ascidian mitochondrial code, and built multiple sequence alignments covering all tunicate classes.Results:All tunicates studied here appear to translate AGR codons to glycine instead of serine (invertebrates) or as a stop codon (vertebrates), as initially described in ascidians. Among Oikopleuridae, we suggest further possible changes in the use of the ATA (Ile → Met) and TGA (Trp → Arg) codons.Conclusions:We recommend using the ascidian mitochondrial code in automatic translation pipelines of mitochondrial sequences for all tunicates. Further investigation is required for additional species-specific differences.
Broadly tunable and low power penalty radio frequency phase shifter using a coupled silicon microcavity
Authors: Pandey Awanish , Selvaraja Shankar Kumar 
Published: December 2019

We present a continuously tunable silicon photonics assisted radio frequency (RF) phase shifter using a coupled microring resonator. Using the coupled cavity, we demonstrate a sub-1 dB power penalty for a RF bandwidth of 34.5 GHz (9–43.5 GHz) and a phase shift of

π

over the reported frequency range. Rigorous optimization of the cavity design using the coupled-mode theory is carried out to realize ultranarrow resonance peaks with a low-extinction ratio and large phase shift. Thermal tuning of the cavity is used to tune the phase while all-optical tuning is exploited to achieve broadband operation. We present a detailed simulation and experimental study of the proposed configuration. The proposed device configuration exhibits a configurable resonance linewidth and extinction ratio that allows for a broad bandwidth and an extremely low power penalty microwave phase shifter. We believe the demonstration would allow better integration of the on-chip functional elements of integrated microwave photonics.
Broadband and flat near-infrared emission from Er3+/Tm3+ codoped tellurite glass for amplifier applications
Authors: Shen Xinjie , Zhu Yarui , Zhou Yaxun , Li Jun 
Published: December 2019

E
r

3
+

/

T
m

3
+

codoped tellurite glasses have been synthesized using the melt-quenching technique; they are characterized by measurements of absorption spectrum, luminescence spectrum, fluorescence decay, Raman spectrum, and differential scanning calorimeter curve. Under the excitation of an 808 nm laser diode (LD), an extremely flat and broadband near-infrared emission with the full width at half-maximum of

∼

160

n
m

has been obtained in tellurite glass doped with optimized

E
r

3
+

−

T
m

3
+

concentrations. This broadband and flat fluorescence emission is located in the region of 1350–1650 nm and covered the E, S, C, and L bands, which originate from the spectral overlapping of 1.53 µm band emission of

E
r

3
+

:

4

I

13

/

2

→

4

I

15

/

2

transition and 1.47 µm band emission of

T
m

3
+

:

3

H

4

→

3

F

4

transition. The Judd-Ofelt theory was applied to the absorption spectrum, and the radiative properties such as transition probability, branching ratio, and radiative lifetime of

E
r

3
+

and

T
m

3
+

ions have been determined to evaluate the potential luminescence characteristics. The time-resolved fluorescence decay of

E
r

3
+

and

T
m

3
+

ions demonstrated the existence of energy transfers between them, and the energy transfer micromechanism was further investigated quantitatively. The results indicate that tellurite glass codoped with appropriate amounts of

E
r

3
+

−

T
m

3
+

ions is promising for applications in broadband optical fibers and photonic devices.
High-contrast, intense single-cycle pulses from an all thin-solid-plate setup
Authors: Seo Meenkyo , Tsendsuren Khurelbaatar , Mitra Sambit , Kling Matthias , Kim Dongeon 
Published: December 2019

High-contrast, intense single-cycle pulses are highly desirable tools in ultrafast science, enabling highest temporal resolution, pushing matter to extreme conditions, and serving as drivers in petahertz electronics. In this Letter, we use thin solid plates in a double multi-plate supercontinuum configuration, delivering a broadband spectrum spanning from

∼

400

to

∼

1000

n
m

at the

−

20

d
B

intensity level to produce a single-cycle pulse. We show that the spectral broadening by self-phase modulation with few-cycle pulses is more suitable for compression than the single-cycle limit than with multi-cycle pulses. The pulses are compressed to 2.6 fs pulses, close to the transform limit of 2.55 fs, with an energy of 0.235 mJ. They exhibit an excellent power stability of 0.5% rms over 3 h and a beam profile. The obtained single-cycle pulses can be utilized in many applications, such as generation of isolated attosecond pulses via high-order harmonic generation, investigation of ultrafast phenomena with extreme temporal resolution, or high-intensity laser-solid experiments.
Distributed cladding mode fiber-optic sensor
Authors: Bashan Gil , London Yosef , Hagai Diamandi H. , Zadok Avi 
Published: December 2019

The analysis of substances outside the cladding has challenged the optical fiber sensors community for decades. A common solution relies on the propagation of light in cladding modes. However, the coupling of light to/from these modes is typically based on permanent gratings in specific locations, which restrict the sensors to point measurements only. In this work, we present dynamic, random-access coupling of light between core and cladding modes of standard fibers, in arbitrarily located short sections. Coupling is based on the stimulation of Brillouin dynamic gratings by two coded pump waves and intermodal scattering of a third optical probe wave. All waves are launched and collected in the core mode. No permanent gratings are inscribed. Distributed sensing of surrounding media is demonstrated with 2 m range and 8 cm resolution. Measurements distinguish between water and ethanol outside the fiber. The measurement accuracy of the local index outside the cladding is 0.004–0.0004 refractive index units.
The 16th Venice Architecture Biennale celebrates the triumph of the ancient régime, but la lutte continue
Authors: Somma Paola 
Published: December 2019

Abstract
In 1968 many intellectuals took to Piazza San Marco to express solidarity with the students of the Venice Academy of Fine Arts who were demonstrating against the Biennale and had been attacked for four hours by the police. In 2018 the same square was patrolled by armed troops deployed to 'protect the tourists' who occupied the city and there was no sign of protests against the Biennale. On the day of inauguration, the only voice of dissent was that of some citizens claiming their right 'to live here'. No trace of intellectuals. Many of the old protesters have made a comfortable career while the invited artists to the international exhibition have uncritically responded to the call of this year's Architecture Biennale, whose title is 'FREESPACE', without questioning the prevailing paradigm in which 'free' means 'space cleared from citizens and offered as a gift to financial investors'. Only a few national pavilions have adopted a more articulate attitude that might recall some of the May '68 aspirations. Retracing the events that transformed a public cultural institution into an enterprise at the service of the art market and a powerful agent of the gentrification and Disneyfication of Venice over the last 50 years, this article focuses on the role played by the Architecture Biennale in the process. It also highlights the few contributions that in 2018 challenged the dominant narrative by explicitly referring to the notion and practice of conflict.
Back to the future? Chinese artistic tradition and topologies of urban modernity
Authors: Becher Angela 
Published: December 2019

Abstract
The radical nature of China's urban transformation has become a key subject in contemporary Chinese art. The ruthless eradication of material remnants of the past, moreover, has reinvigorated an urgency in Chinese art to look to the past for inspiration in the envisioning of a better future. This article examines three works that combine these two important strands of artistic production in China as they negotiate contemporary urban transformation via a return to China´s artistic tradition. This article will look at the imaginary and fantastical topologies of modernity in both analogue and new media, including the installation and oil painting of Shen Yuan and Wang Mingxian, respectively, and the digital ink painting of Miao Xiaochun. In examining closely the artists' choices of medium and their representations of architecture and urban space, this article probes some of the key social, environmental and aesthetic predicaments that underlie China's developmental process. It will argue that responses in Chinese art counter the officially sanctioned grand narrative that equates urbanization, urban renewal and modernization with unequivocal social betterment. Instead, these works create in-between spaces that lie between the material and the idealistic.
Which tradition is mine? Chinese women artists and cultural identity
Authors: Furmanik-Kowalska Magdalena 
Published: December 2019

Abstract
References to native culture are frequently in the foreground of works by Chinese women artists. When they make contact with different cultures, although not necessarily connected with leaving their place of birth thanks to the transfer of information and cultural heritage that has developed extremely efficiently in the era of globalization (Gordon Mathews), they see their own entanglement in the cultural tradition. In the process of constructing their identity they try to find answers to the following question: which part of the cultural tradition is mine? Which one do I identify with? In the case of Chinese women artists, is it the legacy of literati? Classic ink painting and calligraphy? Or perhaps women's crafts that bear no name? Or perhaps a mixture of inspirations? Such questions about material heritage might also be augmented by others that consider aspects of the immaterial heritage of China. This article explores how Chinese women artists such as Chen Qingqing, Qin Yufen, Shi Hui, Wang Xiaohui, Cheng Caroline, Lin Tianmiao, Zhang Yanzi, Man Fung-yi, Liu Liyun, Peng Wei, Chen Lingyang, Chen Qiulin, Zhang Ou and Liu Ren refer to their cultural tradition.
Strategic flexibilities: valuation of a company with the application of the Real Options Theory
Authors: Martinez Izabelle Martinez , Batistela Gislaine Cristina , Simões Danilo 
Published: December 2019

Goal: In this paper, a binomial model is proposed to evaluate the option of deferring an investment and expanding the operational scale of a forest-based company that will perform the de-duplication of Pinus sp. and will market packaging for storage and transportation of vegetables.
Design/Methodology/Approach: The proposed model measured the options of deferring an investment and expanding the operational scale of the forest-based company. In this perspective, the model of evaluation used was the binomial model in discrete time using the Real Options.
Results: It was observed that the inclusion of management flexibilities in the decision making process has added value to the investment project; therefore, the project of investments in real assets proved to be economically feasible.
Limitations of the investigation: The studies that address the corporate finance framework based on real data are a restrictive factor, due to the lack of collaboration of companies, that is, the availability of information that is usually classified.
Practical implications: The study was based on the real data of a company; therefore, it can be adopted as a stimulus to the Real Options approach to the decision making of entrepreneurs or researchers.
Originality/value: The focus of the study was to contemplate the managerial flexibilities of an industry of the secondary sector of the Brazilian economy, which performs the unfolding of wood, demonstrating the innovation of the technique approach used in this market segment.
Blockchain technology applications: a literature review
Authors: Meiriño Marcelo Jasmim , Méxas Mirian Picinini , Faria Alexandre Do Valle , Méxas Rodrigo Picinini , Meirelles Geisa Drumond 
Published: December 2019

Goal: The objective of this article is to raise the state of the art of the applications of blockchain described in the scientific literature.
Design/methodology/approach: A bibliographic research on the Scopus database was carried out from November, 2017 to May, 2018, supported by the scientific research technique called Bibliometry. The compilation of 102 articles found in the scientific literature were presented in the Blockchain context, providing a reference for the fields of blockchain applications.
Results: The results indicate a large increase in the number of articles published since 2017, the largest number of publications were written by authors affiliated with institutions located in China, as well as the most referenced applications are related to cryptocurrencies, smart contracts and IoT.
Limitations of the investigation: The limitation lies in using only the Scopus database for bibliometry, despite being a renowned scientific base.
Practical implications: This study can serve as a bibliographic reference with a global view of the main Blockchain applications that can be applied by different types of organizations
Originality/value: Blockchain is a technology that aims to securely store data, such as financial data, contracts, proof of identity, documents of possession, medical information, etc. To do so, a framework based on laws of mathematics and cryptography is used to ensure the security of transactions of different applications, such as Bitcoin, which is a digital currency that guarantees the safe transfer of financial data. However, being a new technology, there is still little research on the subject. On the other hand, there are different types of applications that are already benefiting from the blockchain structure.
The real options method applied to decision making – an investment analysis
Authors: Benitez Guilherme Brittes , Ferreira Lima Mateus José do Rêgo 
Published: December 2019

Goal: This study aims to assess the impact of using the method of real options in investment analysis through a case study on a retail firm.
Design / Methodology / Approach: It was targeted the applications of the real options method in a different type of environment and it was compared to another method more commonly used, the discounted cash flow method (DCF). The implementation and assessment of the real options method was investigated by means of a case study conducted in an investment analysis in a retail units firm.
Results: The use of the real options method showed a more concise applicability over the DCF method. The results show that the project’s value, after the inclusion of managerial flexibility, increased significantly, which indicates that the analysis of the discounted cash flow undervalued the investment in question, since it disregarded the flexibility to expand or abandon the project.
Limitations of the investigation: The presented method is proper to long-term processes where it is possible to make changes during the project. Investments in this sector usually are more related to short and medium-term decisions, making the application difficult due to the short decision-making period available to the managers.
Practical Implications: The study provided the incorporation of flexibility through different pathways during the building project in a retail units firm. It was showed different scenarios where practitioners could decide among expanding, proceeding, reducing or abandoning the retail units based on the characteristics of their investments.
Originality/value: The results obtained are an indication of this methodology to industrial businesses that are relatively volatile and that need a certain degree of flexibility in order to burgeon, such as the case of the retailing sector.