bluuebunny/crossref_metadata_2025_split huggingface dataset:
The TRPV3 channel of the bovine rumen: localization and functional characterization of a protein relevant for ruminal ammonia transport
Authors: Liebe Franziska , Liebe Hendrik , Kaessmeyer Sabine , Sponder Gerhard , Stumpff Friederike 
Published: May 2020

AbstractLarge quantities of ammonia (NH3 or NH4+) are absorbed from the gut, associated with encephalitis in hepatic disease, poor protein efficiency in livestock, and emissions of nitrogenous climate gasses. Identifying the transport mechanisms appears urgent. Recent functional and mRNA data suggest that absorption of ammonia from the forestomach of cattle may involve TRPV3 channels. The purpose of the present study was to sequence the bovine homologue of TRPV3 (bTRPV3), localize the protein in ruminal tissue, and confirm transport of NH4+. After sequencing, bTRPV3 was overexpressed in HEK-293 cells and Xenopus oocytes. An antibody was selected via epitope screening and used to detect the protein in immunoblots of overexpressing cells and bovine rumen, revealing a signal of the predicted ~ 90 kDa. In rumen only, an additional ~ 60 kDa band appeared, which may represent a previously described bTRPV3 splice variant of equal length. Immunohistochemistry revealed staining from the ruminal stratum basale to stratum granulosum. Measurements with pH-sensitive microelectrodes showed that NH4+ acidifies Xenopus oocytes, with overexpression of bTRPV3 enhancing permeability to NH4+. Single-channel measurements revealed that Xenopus oocytes endogenously expressed small cation channels in addition to fourfold-larger channels only observed after expression of bTRPV3. Both endogenous and bTRPV3 channels conducted NH4+, Na+, and K+. We conclude that bTRPV3 is expressed by the ruminal epithelium on the protein level. In conjunction with data from previous studies, a role in the transport of Na+, Ca2+, and NH4+ emerges. Consequences for calcium homeostasis, ruminal pH, and nitrogen efficiency in cattle are discussed.
Robotic-assisted versus open distal pancreatectomy for benign and low-grade malignant pancreatic tumors: a propensity score-matched study
Authors: Weng Yuanchi , Jin Jiabin , Huo Zhen , Shi Yusheng , Jiang Yu , Deng Xiaxing , Peng Chenghong , Shen Baiyong 
Published: May 2020

Abstract
Background
This study aimed to compare the short-term outcomes of open and robotic-assisted distal pancreatectomy (ODP and RDP) for benign and low-grade malignant tumors.

Methods
The patients who underwent RDP and ODP for benign or low-grade malignant pancreatic tumors at our center were included. After PSM at a 1:1 ratio, the perioperative variations in the two cohorts were compared.

Results
After 1:1 PSM, 219 cases of RDP and ODP were recorded. The RDP cohort showed advantages in the operative duration [120 (90–150) min vs 175 (130–210) min, P < 0.001], estimated blood loss [50 (30–175) ml vs 200 (100–300) ml, P < 0.001], spleen preservation rate (63.5% vs 26.5%, P < 0.001), infection rate (4.6% vs 12.3%, P = 0.006), and gastrointestinal function recovery [3 (2–4) vs. 3 (3–5), P = 0.019]. There were no significant differences in postoperative pancreatic fistula, postoperative hemorrhage, and delayed gastric emptying. Multivariate analysis showed that RDP (HR 0.24; 95% CI 0.16–0.36, P < 0.001), age (HR 1.02; 95% CI 1.00–1.03, P = 0.033), tumor size (HR 1.28; 95% CI 1.17–1.40, P < 0.001), pathological inflammatory neoplasm type (HR 5.12; 95% CI 2.22–11.81, P < 0.001), and estimated blood loss (HR 1.003; 95% CI 1.001–1.004, P < 0.001) were independent predictors of spleen preservation; RDP (HR 0.27; 95% CI 0.17–0.43, P < 0.001), age (HR 1.02; 95% CI 1.00–1.03, P = 0.022), elevated CA 19–9 level (HR 2.55; 95% CI 1.02–6.39, P = 0.046), tumor size (HR 1.44; 95% CI 1.29–1.61, P < 0.001), pathological inflammatory neoplasm type (HR 4.48; 95% CI 1.69–11.85, P = 0.003), and estimated blood loss (HR 1.003; 95% CI 1.001–1.004, P < 0.001) were independent predictors of spleen preservation with the Kimura technique.

Conclusion
RDP has advantages in the operative time, blood loss, spleen preservation, infection rate, and gastrointestinal function recovery over ODP in treating benign and low-grade malignant pancreatic tumors. The robotic-assisted approach was an independent predictor of spleen preservation and use of the Kimura technique.
Impact of literature reports on drug safety signals
Authors: Ochyra Bartlomiej , Szewczyk Maciej , Przybylkowski Adam 
Published: May 2020

Summary
Background
Signal management is considered an important activity in pharmacovigilance and should be performed using any available source of data, including scientific literature. The main aim of this study was to assess the role of scientific literature in both indexed and unindexed journals and compare the relevance of both in the signal management process.

Methods
The study was a retrospective analysis of safety data. For the purposes of the study, drugs for which safety signals were evaluated by European Medicine Agency (EMA) were chosen. A match analysis of data collected in the EudraVigilance (EV) database with data from bibliographic databases such as MEDLINE, Embase or EBSCO (International Pharmaceutical Abstracts, IPA and the Allied and the Complementary Medicine Database, AMED) was performed.

Results
A total of 73 drug event associations (DEA) and 4160 individual case safety reports (ICSRs) were analyzed. About 33% of ICSRs were created based on scientific literature. A total of 1196 ICSRs were submitted to the EV database based on journals indexed in global bibliographic databases Embase (86.00%) or MEDLINE (81.96%) or EBSCO (IPA or AMED, 0.66%).

Conclusion
This study underlines the importance of scientific literature for the signal management process in addition to other data sources. Most literature ICSRs from this analysis were created based on scientific journals indexed in bibliographic databases; therefore, it can be concluded that a systematic review of bibliographic databases, such as Embase or MEDLINE is highly relevant for the signal management process.
Psychotherapeutische Diagnostik aus systemischer Perspektive
Authors: Wagner Elisabeth 
Published: May 2020

ZusammenfassungAm Anfang des Beitrages wird die traditionell diagnosekritische Haltung der systemischen Therapie dargestellt und theoretisch begründet: sowohl aus der interaktionellen Perspektive der frühen Familientherapie, wie auch aus konstruktivistischer und systemtheoretischer Perspektive wird der Anspruch, intrapsychische Störungen objektiv zu erfassen, in Frage gestellt. Der diagnostische Fokus systemischer (Kurz)Therapie liegt dementsprechend auf der Wirklichkeitskonstruktion der_Klientinnen und Klienten, deren Zielen und Ressourcen und soll eine darauf differenziert abgestimmte therapeutische Kooperation ermöglichen.In weiterer Folge wird argumentiert, dass trotz aller berechtigter „Diagnoseskepsis“ nicht zuletzt die Ausweitung des Interventionsrepertoires in der systemischen Therapie eine differenziertere Erfassung intrapsychischer Prozesse als Grundlage verantwortungsvollen therapeutischen Handelns erforderlich macht. Diese wird aus einer synergetischen Perspektive dargestellt und der Klassifikation psychischer Störungen nach ICD und DSM gegenübergestellt. Abschließend werden die Dimensionen eines professionellen systemischen Fallverständnisses zusammenfassend dargestellt.
Comparison of 8-year knee osteoarthritis progression in 2 siblings: a case-based review
Authors: Gourlay Margaret L. , Gourlay Linda L. 
Published: May 2020

AbstractBecause the pathophysiology of knee osteoarthritis is poorly understood, optimal evidence-based clinical management is uncertain. Sibling comparison studies can help inform a clinical model to guide preventive care. We compared the 8-year clinical outcomes in 2 sisters with a family history of osteoarthritis, normal BMI, and absence of knee pain at baseline. Both patients had Kellgren–Lawrence grade 1 in the affected knee at the time of twisting knee injuries leading to osteoarthritis diagnoses at age 50 (patient 1) and 51 (patient 2). Patient 1 developed a chronic right knee effusion, and progressed to Kellgren–Lawrence grade 3 bilaterally by the time she had a right total knee replacement at age 58. Patient 2 had subchondral fractures of the right knee with transient effusion, which healed after 1 year of partial weight-bearing with crutches and subsequent daily use of knee sleeves. Patient 2 had Kellgren–Lawrence grade 0 bilaterally upon surveillance imaging at age 58. The terms “osteoarthritis and knee and diagnostic imaging and subchondral bone and pathophysiology” were searched in the PubMed database to identify original research articles to inform a clinical model consistent with the patients’ outcomes. A fluid model of osteoarthritis was the best explanatory model for the discordant clinical trajectories of the age-matched siblings. Patient recommendations are presented based on these findings.
Prognostic value of preoperative hematologic biomarkers in urothelial carcinoma of the bladder treated with radical cystectomy: a systematic review and meta-analysis
Authors: Mori Keiichiro , Miura Noriyoshi , Mostafaei Hadi , Quhal Fahad , Motlagh Reza Sari , Lysenko Ivan , Kimura Shoji , Egawa Shin , Karakiewicz Pierre I. , Shariat Shahrokh F. 
Published: May 2020

AbstractThis systematic review and meta-analysis aimed to assess the prognostic value of preoperative hematologic biomarkers in patients with urothelial carcinoma of the bladder treated with radical cystectomy. PUBMED, Web of Science, Cochrane Library, and Scopus databases were searched in September 2019 according to the Preferred Reporting Items for Systematic Review and Meta-analysis statement. Studies were deemed eligible if they compared cancer-specific survival in patients with urothelial carcinoma of the bladder with and without pretreatment laboratoryabnormalities. Formal meta-analyses were performed for this outcome. The systematic review identified 36 studies with 23,632 patients, of these, 32 studies with 22,224 patients were eligible for the meta-analysis. Several preoperative hematologic biomarkers were significantly associated with cancer-specific survival as follows: neutrophil − lymphocyte ratio (pooled hazard ratio [HR]: 1.20, 95% confidence interval [CI]: 1.11–1.29), hemoglobin (pooled HR: 0.87, 95% CI 0.82–0.94), C-reactive protein (pooled HR: 1.44, 95% CI 1.26–1.66), De Ritis ratio (pooled HR: 2.18, 95% CI 1.37–3.48), white blood cell count (pooled HR: 1.05, 95% CI 1.02–1.07), and albumin-globulin ratio (pooled HR: 0.26, 95% CI 0.14–0.48). Several pretreatment laboratory abnormalities in patients with urothelial carcinoma of the bladder were associated with cancer-specific mortality. Therefore, it might be useful to incorporate such hematologic biomarkers into prognostic tools for urothelial carcinoma of the bladder. However, given the study limitations including heterogeneity and retrospective nature of the primary data, the conclusions should be interpreted with caution.
The excess economic burden of mental disorders: findings from a cross-sectional prevalence survey in Austria
Authors: Łaszewska Agata , Wancata Johannes , Jahn Rebecca , Simon Judit 
Published: May 2020

AbstractInformation about the scope of mental disorders (MDs), resource use patterns in health and social care sectors and economic cost is crucial for adequate mental healthcare planning. This study provides the first representative estimates about the overall utilisation of resources by people with MDs and the excess healthcare and productivity loss costs associated with MDs in Austria. Data were collected in a cross-sectional survey conducted on a representative sample (n = 1008) between June 2015 and June 2016. Information on mental health diagnoses, 12-month health and social care use, medication use, comorbidities, informal care, early retirement, sick leave and unemployment was collected via face-to-face interviews. Generalised linear model was used to assess the excess cost of MDs. The healthcare cost was 37% higher (p = 0.06) and the total cost was twice as high (p < 0.001) for the respondents with MDs compared to those without MDs. Lost productivity cost was over 2.5-times higher (p < 0.001) for those with MDs. Participants with severe MDs had over 2.5-times higher health and social care cost (p < 0.001) and 9-times higher mental health services cost (p < 0.001), compared to those with non-severe MDs. The presence of two or more physical comorbidities was a statistically significant determinant of the total cost. Findings suggest that the overall excess economic burden on health and social care depends on the severity of MDs and the number of comorbidities. Both non-severe and severe MDs contribute to substantially higher loss productivity costs compared to no MDs. Future resource allocation and service planning should take this into consideration.
Exploring the desiccation tolerance of the invasive bivalve Corbicula fluminea (Müller 1774) at different temperatures
Authors: Guareschi Simone , Wood Paul J. 
Published: May 2020

AbstractCorbicula fluminea (Müller, 1774) (Mollusca, Corbiculidae) is among the most successful and widespread invaders of aquatic ecosystems worldwide. Given its wide geographical distribution and documented effects on aquatic ecosystems, there is considerable interest in quantifying its behaviour, propagation and control. We conducted a series of laboratory experiments to assess the effects of complete desiccation (aerial exposure at high relative humidity) on a UK population of C. fluminea for: (1) different environmental temperatures (6 scenarios: from cool winter droughts to summer heatwave thermal conditions); and (2) two size classes (> 1 cm and ≤ 1 cm). A mortality rate of 100% was obtained for all experiments, except the lowest temperature scenario of 4 °C. For both high-temperature scenarios (25 and 30 °C) 100% mortality was recorded after 48 h, and an elevated mortality rate recorded after 24 h. An extended period of desiccation of 5–6 days would be necessary to reach a mortality rate of 90% at 15 °C and 3.5 days at 20 °C. Statistical analysis demonstrated a significant effect of temperature on mortality, but both size classes displayed similar responses to desiccation. The greatest difference in mortality was recorded between the highest and lowest experimental temperatures. C. fluminea was particularly tolerant to desiccation when low temperature and high humidity conditions coincided, suggesting it could potentially spread to regions beyond its current eco-geographical range. The results will be of direct interest for regulatory authorities considering desiccation (and water level management) as a means of managing and preventing the further spread of this species.
Climate change and small pelagic fish price volatility
Authors: Pincinato Ruth Beatriz Mezzalira , Asche Frank , Oglend Atle 
Published: May 2020

AbstractPrice volatility, an indicative measure of price uncertainty, is an important factor influencing fisheries cost, income, food security, and pressure on fish stocks. An increase in price volatility means that prices are varying more, making it more difficult to adjust the activities accordingly. This paper explores price volatility changes for the small pelagic fish—a key group for food security—related to the potential outcomes of climate change, such as tropical widening. With the tropicalization of temperate and polar marine ecosystems due to species composition changes, one would expect that overall price volatility for the small pelagics may be reduced over time because volatility in the tropical region is on average the lowest. However, we find an empirical evidence that price volatility for small pelagic species has increased relatively in the tropical and sub-polar regions beyond what can be explained by changes in landing levels and the variation in the landings.
Atypical Topographical Organization of Global Form and Motion Processing in 5-Month-Old Infants at Risk for Autism
Authors: Nyström Pär , Jones Emily , Darki Fahimeh , Bölte Sven , Falck-Ytter Terje 
Published: May 2020

AbstractResearch indicates that individuals with autism spectrum disorder (ASD) are superior at local processing while the integration of local features to global percepts is reduced. Here, we compared infants at familiar risk for ASD to typically developing infants in terms of global coherence processing at 5 months of age, using steady state visually evoked potentials (SSVEP). We found a different topographical organization for global form and motion processing in infants at risk (n = 50) than in controls (n = 23). In contrast, activation patterns for local visual change were strikingly similar between groups. Although preliminary, the results represent the first neurophysiological evidence supporting the view that basic atypicalities in perception may play a role in the developmental pathways leading to ASD.
Perioperative echocardiography-guided hemodynamic therapy in high-risk patients: a practical expert approach of hemodynamically focused echocardiography
Authors: Trauzeddel R. F. , Ertmer M. , Nordine M. , Groesdonk H. V. , Michels G. , Pfister R. , Reuter D. , Scheeren T. W. L. , Berger C. , Treskatsch S. 
Published: May 2020

AbstractThe number of high-risk patients undergoing surgery is growing. To maintain adequate hemodynamic functioning as well as oxygen delivery to the vital organs (DO2) amongst this patient population, a rapid assessment of cardiac functioning is essential for the anesthesiologist. Pinpointing any underlying cardiovascular pathophysiology can be decisive to guide interventions in the intraoperative setting. Various techniques are available to monitor the hemodynamic status of the patient, however due to intrinsic limitations, many of these methods may not be able to directly identify the underlying cause of cardiovascular impairment. Hemodynamic focused echocardiography, as a rapid diagnostic method, offers an excellent opportunity to examine signs of filling impairment, cardiac preload, myocardial contractility and the function of the heart valves. We thus propose a 6-step-echocardiographic approach to assess high-risk patients in order to improve and maintain perioperative DO2. The summary of all echocardiographic based findings allows a differentiated assessment of the patient's cardiovascular function and can thus help guide a (patho)physiological-orientated and individualized hemodynamic therapy.
Influence of water matrix and hydrochar properties on removal of organic and inorganic contaminants
Authors: Niinipuu Mirva , Bergknut Magnus , Boily Jean-François , Rosenbaum Erik , Jansson Stina 
Published: May 2020

AbstractThe removal of contaminants from water using low-cost adsorbents has been widely studied, yet studies employing a realistic water matrix are still lacking. This study investigated the removal of organic compounds (trimethoprim, fluconazole, and perfluorooctanoic acid (PFOA)) and metals (As, Zn, and Cu) from landfill leachate. Additionally, tests in pure water, humic acid, and ion matrices were carried out to better understand how the water matrix affects adsorption. The hydrochars were produced from four feedstocks at three carbonization temperatures. The results show that the removal efficiencies for organic pollutants were low and metal removal by hydrochars was comparable with commercial activated carbon. The removal of all compounds from pure water was substantially lower. Tests with humic acid and ion-containing matrices could not fully explain the increased removal in the landfill leachate, which may be due to the combination of the water matrix and presence of soluble species from the hydrochars.
Cyanotoxin release from the benthic, mat-forming cyanobacterium Microseira (Lyngbya) wollei in the St. Lawrence River, Canada
Authors: Poirier-Larabie Sylvie , Hudon Christiane , Poirier Richard Hugo-Pierre , Gagnon Christian 
Published: May 2020

AbstractBenthic cyanobacterial mats occurring in the St. Lawrence River fluvial lakes Saint-Louis and Saint-Pierre are dominated by Microseira (Lyngbya) wollei which produce several cyanotoxins including LWTX-1 that is characteristic of Microseira wollei. This cyanotoxin is not only present in the filaments forming benthic mats, but was also measured in the water overlying the mats. LWTX-1 was found in all cyanobacterial filament samples (75.29–103.26 ng mg−1) and all overlying water samples (3.01–11.03 ng L−1). Toxin concentrations measured in overlying water and dry biomass were strongly correlated (r = 0.94). Furthermore, LWTX-1 concentration in water was positively correlated with the dissolved organic carbon in water (r = 0.74) and % nitrogen content in cyanobacterial filaments (r = 0.52). A preliminary study was conducted to determine the release and degradation rates of LWTX-1 from a M. wollei mat kept under laboratory conditions over a 3-month period. Toxin measurements revealed an early, massive toxin release followed by a typical decaying function, with a half-life in the order of 17 days. Our results raise concerns about the occurrence and downstream advection of dissolved cyanotoxins from Microseira mats in the aquatic environment.
Cell-Scale Degradation of Peritumoural Extracellular Matrix Fibre Network and Its Role Within Tissue-Scale Cancer Invasion
Authors: Shuttleworth Robyn , Trucu Dumitru 
Published: May 2020

AbstractLocal cancer invasion of tissue is a complex, multiscale process which plays an essential role in tumour progression. During the complex interaction between cancer cell population and the extracellular matrix (ECM), of key importance is the role played by both bulk two-scale dynamics of ECM fibres within collective movement of the tumour cells and the multiscale leading edge dynamics driven by proteolytic activity of the matrix-degrading enzymes (MDEs) that are secreted by the cancer cells. As these two multiscale subsystems share and contribute to the same tumour macro-dynamics, in this work we develop further the model introduced in Shuttleworth and Trucu (Bull Math Biol 81:2176–2219, 2019. 10.1007/s11538-019-00598-w) by exploring a new aspect of their interaction that occurs at the cell scale. Specifically, here we will focus on understanding the cell-scale cross talk between the micro-scale parts of these two multiscale subsystems which get to interact directly in the peritumoural region, with immediate consequences both for MDE micro-dynamics occurring at the leading edge of the tumour and for the cell-scale rearrangement of the naturally oriented ECM fibres in the peritumoural region, ultimately influencing the way tumour progresses in the surrounding tissue. To that end, we will propose a new modelling that captures the ECM fibres degradation not only at macro-scale in the bulk of the tumour but also explicitly in the micro-scale neighbourhood of the tumour interface as a consequence of the interactions with molecular fluxes of MDEs that exercise their spatial dynamics at the invasive edge of the tumour.