bluuebunny/crossref_metadata_2025_split huggingface dataset:
Allogeneic haematopoietic stem cell transplantation can improve the prognosis of high-risk paediatric t(8;21) acute myeloid leukaemia in first remission based on MRD-guided treatment.
Authors: Hu Guan-hua , Cheng Yi-fei , Lu Ai-dong , Wang Yu , Zuo Ying-xi , Yan Chen-hua , Wu Jun , Sun Yu-qian , Suo Pan , Chen Yu-hong , Chen Huan , Jia Yue-ping , Liu Kai-yan , Han Wei , Xu Lan-ping , Zhang Leping , Huang Xiao-jun 
Published: May 2020

Abstract
Background: Pediatric acute myeloid leukemia (AML) with t(8;21) (q22;q22) is classified as a low-risk group. However, relapse is still the main factor affecting survival. We aimed to investigate the effect of allogeneic hematopoietic stem cell transplantation (allo-HSCT) on reducing recurrence and improving the survival of high-risk pediatric t(8;21) AML based on minimal residual disease (MRD)-guided treatment, and to further explore the prognostic factors to guide risk stratification treatment and identify who will benefit from allo-HSCT. Methods: Overall, 129 newly diagnosed pediatric t(8;21) AML patients were included in this study. Patients were divided into high-risk and low-risk group according to RUNX1-RUNX1T1 transcript levels after two cycles of consolidation chemotherapy. High-risk patients were divided into HSCT group and chemotherapy group according to their treatment choices. The characteristics and outcomes of 125 patients were analyzed. Results: For high-risk patients, allo-HSCT could improve 5-year relapse-free survival (RFS) rate compared to chemotherapy (87.4% vs. 61.9%; P = 0.026). Five-year overall survival (OS) rate in high-risk HSCT group had a trend for better than that in high-risk chemotherapy group (82.8% vs. 71.4%; P = 0.260). The 5-year RFS rate of patients with a c-KIT mutation in high-risk HSCT group had a trend for better than that of patients with a c-KIT mutation in high-risk chemotherapy group (82.9% vs. 75%; P = 0.400). Extramedullary infiltration (EI) at diagnosis was associated with a high cumulative incidence of relapse for high-risk patients (50% vs. 18.4%; P = 0.004); allo-HSCT can improve the RFS (P = 0.009). Conclusions: allo-HSCT can improve the prognosis of high-risk pediatric t(8;21) AML based on MRD-guided treatment. Patients with a c-KIT mutation may benefit from allo-HSCT. EI is an independent prognostic factor for high-risk patients and allo-HSCT can improve the prognosis.
Validation Of IGBO Version Of The Modified Falls Efficacy Scale Among Community-Dwelling Older Adults: A Validation Study
Authors: Okoye Emmanuel Chiebuka , Akosile Christopher Olusanjo , Maruf Fatai Adesina , Onwuakagba Ifeoma Uchenna , Urama Sunday Tobias 
Published: May 2020

Abstract
Background: Fear of falling (FOF) is a very pervasive problem among older adults. Consequently, many scales have been developed for its assessment. The Modified Fall Efficacy Scale (MFES) is one of the most popular FOF scales. The MFES was originally developed for use in developed countries, and thus may not be entirely suitable for use in developing countries due to cultural and environmental differences between the two country categories. This study was therefore designed to cross-culturally adapt and validate the MFES to Igbo culture and environment among community-dwelling older adults in Nnewi community using established guidelines.Methods: The original English version of the MFES (E-MFES) was translated, synthesized, back-translated, subjected to expert panel review, and pretested before producing the final Igbo version of the MFES (I-MFES). The E-MFES, the I-MFES and the Short Falls Efficacy International were randomly administered to consecutively recruited 109 consenting older adult residents of Nnewi (43.1% males; mean age=74.45±8.78 years). Concurrent, convergent and structural validities and internal consistency of the I-MFES were assessed at 0.05 level of significance. Results: All the 14 items on the E-MFES were retained on the I-MFES. There was no significant difference between the corresponding scores on the E-MFES and the I-MFES (p<0.05). The correlations between the corresponding scores on the I-MFES and the M-FES were all excellent (r=0.97-1.00) indicating evidence of concurrent validity of the I-MFES. The correlation between the total scores on the I-MFES and the Short Falls Efficacy International was excellent (r=9.3) indicating evidence of convergent validity. The Cronbach’s alpha value of the I-MFES is 0.97 showing evidence of excellent internal consistency. Conclusion: This study provides evidence of some aspects of validity and reliability of the I-MFES.
The Pulmonary Sequalae in Discharged Patients With COVID-19: A Short-term Observational Study
Authors: Liu Dehan , Zhang Wanshu , Pan Feng , Li Lin , Yang Lian , Wang Jiazheng , Zheng Dandan , Liang Bo 
Published: May 2020

Abstract
Background: A cluster of patients with coronavirus disease 2019 (COVID-19) pneumonia were discharged from hospitals in Wuhan, China. We aimed to determine the cumulative percentage of complete radiological resolution at each time point, to explore the relevant affecting factors, and to describe the chest CT findings at different time points after hospital discharge.Methods: Patients with COVID-19 pneumonia confirmed by RT-PCR who were discharged consecutively from the hospital between 5 February 2020 and 10 March 2020 and who underwent serial chest CT scans on schedule were enrolled. The radiological characteristics of all patients were collected and analysed. The total CT score was the sum of non-GGO involvement determined at discharge. Afterwards, all patients underwent chest CT scans during the 1st, 2nd, and 3rd weeks after discharge. Imaging features and distributions were analysed across different time points.Results: A total of 149 patients who completed all CT scans were evaluated; there were 67 (45.0%) men and 82 (55.0%) women, with a median age of 43 years old (IQR 36-56). The cumulative percentage of complete radiological resolution was 8.1% (12 patients), 41.6% (62), 50.3% (75), and 53% (79) at discharge and during the 1st, 2nd, and 3rd weeks after discharge, respectively. Patients ≤44 years old showed a significantly higher cumulative percentage of complete radiological resolution than patients >44 years old at the 3-week follow-up. The predominant patterns of abnormalities observed at discharge were ground-glass opacity (GGO) (65 [43.6%]), fibrous stripe (45 [30.2%]), and thickening of the adjacent pleura (16 [10.7%]). Lung lesions showed obvious resolution from 2 to 3 weeks after discharge, especially in terms of GGO and fibrous stripe. “Tinted” sign and bronchovascular bundle distortion as two special features were discovered during the evolution.Conclusion: Lung lesions in COVID-19 pneumonia patients can be absorbed completely during short-term follow-up with no sequelae. Three weeks after discharge might be the optimal time point for early radiological estimation.
Biodegradation of textile dye using consortium of bacterial strains isolated from industrial dye effluent
Authors: El-Sheshtawy H. S. , Abdelkreem M. 
Published: May 2020

Abstract
Dyes are discharged into water through many industries such as textile industries. Disposal of these dyes into the aquatic and terrestrial environment causes serious damage. Remarkable, processes for the treatment of dye waste involve biological remediation, catalytic oxidation, filtration, and sorption process. In this study the textile industrial dye effluent was treated using biodegradation method. The degradation of dye wastewater was determined by pure bacteria and its consortium isolated from textile effluent. Three different bacterial spp. were isolated and subjected to dye decolorization assays. The isolates were identified by 16sRNA as Aeromonas aquatic AE 235, Flavobacterium ginsengiterrae DCY55, Shewanella xiamenensis S4. The percentage decolorization process was detected by UV spectrophotometer. This process was obtained at 90, 94, 95 and 98% respectively by pure of three bacterial strains B1, B2, and B3 and its consortium BC. The degradation process of dye effluent was confirmed by FTIR analysis. The physico-chemical analysis of the effluent sample was investigated before and after biodegradation treatment to emphasize the process efficiency. The results indicated that these bacterial strains have good potential for the biodegradation of dye from textile waste water.
Identification of ACSL4 as a biomarker and contributor of ferroptosis in clear cell renal cell carcinoma
Authors: guo na 
Published: May 2020

Abstract
Background

ACSL4 has been reported to be related to tumor genesis and involved in the processes of ferroptosis. However, the expression levels and prognostic value of ACSL4 in clear cell renal cell carcinoma (ccRCC) remain unclear.
Methods

The Oncomine and TCGA databases were used to predict the expression of ACSL4 mRNA in ccRCC and its association with ccRCC prognosis. The expression levels of ACSL4 were determined in human RCC tissues by real-time PCR. Kaplan-Meier curves were used to analyze the diagnostic and prognostic significance of ACSL4 in ccRCC. A ferroptosis inducer (erastin) was used to investigate the effects of ACSL4 on ferroptosis in ccRCC cell lines.
Results

The expression level of ACSL4 was significantly down-regulated in ccRCC tissues (P < 0.001), which was consistent with the analysis of the Oncomine and TCGA database. Then, immunohistochemical results demonstrated that the ACSL4 was weak or not detected in ccRCC tissues than that in normal tissues. ACSL4 differential expression level was significantly related to gender, ccRCC subtypes, nodal invasion, tumor grade and cancer stages (all P < 0.001). Survival analysis revealed that overall survival was favorable in ccRCC patients with ACSL4 high expression (P = 0.014). Overexpression of ACSL4 by gene transfection restores ferroptosis sensitization in cancer cells, whereas suppression of ACSL4 expression by RNAi increases ferroptosis resistance. Mechanically, protein ubiquitination may be involved in ACSL4-mediated ferroptosis.
Conclusions

As a monitor and contributor of ferroptosis, ACSL4 was decreased in ccRCC and served as a useful diagnostic and prognostic biomarker, which will be a new potential therapeutic target for ccRCC.
Intramedullary reaming and irrigation in associated with antibiotic-loaded calcium sulfate implantation in the treatment of infection after intramedullary nailing: A retrospective study of 19 cases.
Authors: Zhang Hong-An , Zhou Chun-Hao , Meng Xiang-Qing , Fang Jia , Qin Cheng-He 
Published: May 2020

Abstract
Background
The incidence of intramedullary infection is increasing with the frequent application of intramedullary fixations on long bone fractures in latest decades. However, appropriate treatment for those special infection remains a challenge. The aims of this study were to assess the efficiency of our treatment protocols: intramedullary nail removal, medullary canal reaming and irrigation, followed by antibiotic-loaded calcium sulfate implantation with or without distraction osteogenesis, for the treatment of infection after intramedullary nailing.
Methods
From 2014 to 2017, a total of 19 patients with intramedullary infection were treated in our center, with means of intramedullary nail removal, distal diaphysis fenestration, medullary canal reaming and irrigation, antibiotic-loaded calcium sulfate implantation, followed by distraction osteogenesis in 9 cases to repair bone defect. The infection remission rate, infection recurrence rate and post-operative complications rate were assessed during the follow-up.
Results
All of patients gained satisfactory outcomes with an average follow-up of 38.1 (24 to 55months). 94.7% (18/19) patients achieved infection remission after surgical treatment. 5.3% (1/19) patient developed reinfection, but healed at the end of follow up with re-debridement. 9 patients with surgery-related bone defects received bone transport and successfully restored the length of involved limbs, with a mean transport duration of 10.7 months (range, 6.7 to 19.5months). Majority of patients achieved pain free and full weight bearing during the follow-up. Postoperative complications mainly included prolonged aseptic draining (36.8%, 7/19) and refracture (5.3%, 1/19), which were successfully managed by regular dressing and refixation.
Conclusion
Intramedullary nail removal, canal reaming and irrigation in associated with antibiotic-loaded calcium sulfate implantation (with or without distraction osteogenesis) were effective in the treatment of infection after intramedullary nailing.
Propensity for COVID-19 severe epidemic among the populations of the neighborhoods of Fortaleza, Brazil, in 2020
Authors: Braga Jose Ueleres , Ramos Alberto Novaes , Ferreira Anderson Fuentes , Lacerda Victor Macêdo , Freire Renan Monteiro Carioca , Bertoncini Bruno Vieira 
Published: May 2020

Abstract
Background: The state of Ceará (Northeast Brazil) has shown a high incidence of coronavirus disease (COVID-19), and most of the cases that were diagnosed during the epidemic originated from the capital Fortaleza. Monitoring the dynamics of the COVID-19 epidemic is of strategic importance and requires the use of sensitive tools for epidemiological surveillance, including consistent analyses that allow the recognition of areas with a greater propensity for increased severity throughout the cycle of the epidemic. This study aims to classify neighborhoods in the city of Fortaleza according to their propensity for a severe epidemic of COVID-19 in 2020. Methods: We conducted an ecological study within the geographical area of the 119 neighborhoods located in the city of Fortaleza. To define the main transmission networks (infection chains), we assumed that the spatial diffusion of the COVID-19 epidemic was influenced by population mobility. To measure the propensity for a severe epidemic, we calculated the infectivity burden (ItyB), infection burden (IonB), and population epidemic vulnerability index (PEVI). The propensity score for a severe epidemic in the neighborhoods of the city of Fortaleza was estimated by combining the IonB and PEVI. Results: The neighborhoods with the highest propensity for a severe COVID-19 epidemic were Aldeota, Cais do Porto, Centro, Edson Queiroz, Vicente Pinzon, Jose de Alencar, Presidente Kennedy, Papicu, Vila Velha, Antonio Bezerra, and Cambeba. Importantly, we found that the propensity for a COVID-19 epidemic was high in areas with differing socioeconomic profiles. These areas include a very poor neighborhood situated on the western border of the city (Vila Velha), neighborhoods characterized by a large number of subnormal agglomerates in the Cais do Porto region (Vicente Pinzon), and those located in the oldest central area of the city, where despite the wealth, low-income groups have remained (Aldeota and the adjacent Edson Queiroz).Conclusion: Although measures against COVID-19 should be applied to the entire municipality of Fortaleza, the classification of neighborhoods generated through this study can help improve the specificity and efficiency of these measures.
Preparing for the COVID-19 Pandemic Response in a Country Emerging From an Ebola Epidemic: Assessment of Health Workers' Knowledge, Attitudes and Practices on Coronavirus (COVID-19) in Guinea
Authors: CAMARA Ibrahima 
Published: May 2020

Abstract
Background: On January 30, 2020, the World Health Organization designated the outbreak as a Public Health Emergency of International Scope (USPPI). Underdeveloped countries, such as the Republic of Guinea, where the health system has been severely impacted by the Ebola virus epidemic between 2013 and 2014, will therefore find it difficult to respond effectively to the predicted epidemic. The purpose of this study was to assess the knowledge, attitudes and practices of medical personnel on the prevention of Covid-2019.Methods:This was a cross-sectional study with an analytical aim carried out from 1st to 29th February 2020 among front-line health workers in the health structures of the city of Conakry. Data were collected using the Kobocollect application by administration of a standardized questionnaire. The knowledge and attitude score was categorized in 2: Good if ≥ at the average score and bad if < 50%. Logistic regression models were conducted to identify factors associated with knowledge and attitude. Odds ratios (OR) with their confidence intervals were calculated.Results :548 health workers surveyed, the mean score for knowledge on the prevention of Covid-19 was 54.81 SD = 9.71, [95% CI: [ 53.9-55.6]. 70.6% had a good knowledge of Covid-19. However, 99.6% of the staff did not know the emergency number in case of a suspected case and about 50% did not know the incubation period of Covid-19.Among the health personnel interviewed, 57.7% had a good attitude towards a suspected case of Covid-19.Although the majority (57.5%) of the health workers had received training in infection prevention and control, infection prevention and control (IPC) practices were not routinely implemented, as the implementation rate was not even 80% for every action.Variables associated with poor knowledge of Covid-19 prevention were sex, health facility, and staff grade. As for poor attitude, the associated variables were knowledge of Ebola cases reported in the facility and the rank of the health staff.Conclusion:This study underlines the difficulties of the appropriation of prevention and protection measures against Covid-19 by health personnel for whom the risk appears remote and limited to a transnational or foreign elite.
Evaluation of Youtube Video Content About Developmental Dysplasia of the Hip
Authors: Oztermeli Ahmet , Karahan Nazım 
Published: May 2020

Abstract
Background: The purpose of this study is to investigate the quality and reliability of YouTube videos regarding developmental dysplasia of the hip (DDH).YouTube is one of the most popular websites used as a source of information, but the variety in authorship and lack of a peer-review process are problems.Methods: The search string “developmental dysplasia of the hip” was inputted to the YouTube search engine, and the first 52 videos returned as a response were assessed. The Video Power Index (VPI) (like ratio*view ratio/100) was used to assess the popularity of the videos. Global Quality Score (GQS) and developmental dysplasia of the hip scores (DDHS) were used to evaluate the quality and educational quality of the videos, and The Journal of the American Medical Association Score (JAMAS) was used to evaluate the accuracy of the source of information.Results: According to our research, the mean duration time of the videos was 526 s (SD: 813), and the average view count of the videos was 34,644. The mean time since upload was 1907 days (SD: 1137). On average, the videos received 10.9 comments, 210.3 likes, and 6.8 dislikes. The mean like ratio and VPI were 92.9 (SD: 19.57) and 25.8 (SD: 53.43), respectively. The mean JAMAS, GQS, and DDHS of all videos evaluated were 1.37 (SD: 0.7), 2.46 (SD: 1.09), and 4.63 (SD: 5.00), respectively. The DDHS and GQS were positively correlated (p:0.001 r: 65.8%). The GQS and the DDHS were higher in the academic group than in the commercial group (p:0.01). (p:0.037).Conclusions: The videos regarding DDH on YouTube generally had poor quality. As a result, to maintain an optimal parent–physician or patient–physician relationship, we suggest that international health societies make their own educational videos for parents, patients, and fellow physicians.
Dendrobium officinale Six nostrum promotes excretion of intestinal uric acid in hyperuricemia rat through inhibiting LPS/TLR4/NF-κB signaling pathway
Authors: xue chen , hongzhang ge , shanshan lei , zhetian jiang , jie su , qiaoxian yu , bo li , guiyuan lv , suhong chen 
Published: May 2020

Abstract
Background : To evaluate the effect of Dendrobium officinale Six nostrum (DOS) on promoting the intestinal excretion of uric acid (UA) in the rat model of hyperuricemia (HUA), and explored its possible mechanisms of action. Methods: In this study, HUA was induced in rat by administration of lipid emulsion (LE) for 6 weeks, meanwhile, the rat was orally administered with DOS for 6 weeks. Then the level of serum uric acid (SUA) and fecal uric acid (FUA) were measured by automatic biochemical analyzer. Lipopolysaccharide (LPS) in hepatic portal vein blood was detected by ELISA kit. The intestinal protein levels of ATP-binding cassette superfamily G member 2 (ABCG2) and glucose transporter 9 (GLUT9) were measured by Western blot assay. Toll-like receptor 4 (TLR4) and nuclear factor kappa-B (NF-κB) were determined by immunohistochemistry (IHC). Hematoxylin and eosin (H&E) staining was used to assess intestinal histological changes. Meanwhile, the main compositions of DOS were identified and determined by the High Performance Liquid Chromatography (HPLC). Results: According to HPLC analysis, acteoside and astilbin were identified as the main chemical components of DOS. Our results indicated that DOS significantly reduced SUA level and increased FUA level in the HUA rat induced by oral administration of LE for 6 weeks. IHC and Western blot showed that DOS could significantly increase protein level of ABCG2 and reduce protein level of GLUT9 in the intestine. It remarkably reduced the content of LPS in hepatic portal vein blood and decrease protein levels of TLR4 and NF-κB in the intestine. In addition, DOS could improve the histopathological changes of intestine through increasing the number of intestinal gland goblet cells, and recovering the complete and neatly-arranged structure of small intestinal epithelial cells. Conclusions: DOS has the effect of treating hyperuricemia, the molecular mechanism may be associated with up-regulating ABCG2 protein level, and down-regulating protein level of GLUT9 in the intestine to promote the intestinal excretion of UA, and then decreasing the SUA level. In addition, DOS can reduce the damage of inflammatory response to the intestine, improve the histopathological changes of intestine in HUA rat through inhibiting LPS/TLR4/NF-κB inflammatory pathway.