bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci
Authors: Satofuka Hiroyuki , Abe Satoshi , Moriwaki Takashi , Okada Akane , Kazuki Kanako , Tanaka Hiroshi , Yamazaki Kyoutarou , Hichiwa Genki , Morimoto Kayoko , Takayama Haruka , Nakayama Yuji , Hatano Shinya , Baba Yoshihiro , Oshimura Mitsuo , Tomizuka Kazuma , Kazuki Yasuhiro 
Published: December 2020

Abstract
Trans-chromosomic (Tc) mice carrying mini-chromosomes with human immunoglobulin (Ig) loci have contributed to the development of fully human therapeutic monoclonal antibodies (Abs); however, we previously observed that mitotic instability of human mini-chromosomes in mice has limited the efficiency of hybridoma production. Here, we established a new generation of human Ab producing Tc mice (TC-mAb mice), which stably maintain a mouse-derived engineered chromosome containing the entire human Ig heavy and kappa chain loci in a mouse Ig knockout background. Comprehensive, high-throughput DNA sequencing revealed that the human Ig repertoire, including variable gene use, was well recapitulated in TC-mAb mice. Despite slightly altered B cell development and a delayed immune response, immunized TC-mAb mice exhibited more subsets of antigen-specific plasmablast and plasma cells compared with wild-type mice, leading to high efficiency hybridoma production. Thus, TC-mAb mice offer a valuable platform to obtain fully human therapeutic Abs and to elucidate the regulation of human Ig repertoire formation.
Early growth response 2 regulates mouse cooperative behavior
Authors: Xiao Ming , Zhang Yanli , Feng Weixi , Wang Ze , Wang Tianqi , Pang Yingting , Jin Yuxi , Zou Ying , Chen Yan , Li Qian , Huang Huang , Sheng Chengyu 
Published: December 2020

Abstract
Social cooperation is essential to animals’ physical health and psychological state. However, the underlying molecular and neurobiological mechanisms remain poorly understood. Here, we established a novel model for systematically evaluating the cooperative behavior of mice for the task of water reward. Using this paradigm, we characterized cooperative deficits in isolated juvenile mice and the corrective effect of resocialization. Mechanistically, we found that the transcription factor early growth response 2 (Egr2)-dependent myelin maturation in the medial prefrontal cortex is necessary for the development of mice’s cooperative behavior. Additionally, corticosterone levels in the serum and medial prefrontal cortex were elevated in the isolated mice, potentially contributing to Egr2 expression reduction. This work suggests targeting Egr2 could be targeted for preventing and treating social isolation-related neuropsychiatric disorders in the future.
Superconductivity beyond Pauli's limit in bulk NbS2: Evidence for the Fulde-Ferrell-Larkin-Ovchinnikov state
Authors: Cho Chang-woo , Lyu Jian , Ng Cheuk , He James Jun , Abdel-Baset Tarob , Abdel-Hafiez Mahmoud , Lortz Rolf 
Published: December 2020

Abstract
We present magnetic torque, specific heat and thermal expansion measurements combined with a piezo rotary positioner of the bulk transition metal dichalcogenide (TMD) superconductor NbS2 in high magnetic fields applied strictly parallel to its layer structure. The upper critical field of superconducting TMDs in the 2D form is known to be dramatically enhanced by a special form of Ising spin orbit coupling. This Ising superconductivity is very robust against the Pauli limit for superconductivity. We find that superconductivity beyond the Pauli limit still exists in bulk single crystals of NbS2. However, the comparison of our upper critical field transition line with numerical simulations rather points to the development of a Fulde-Ferrell-Larkin-Ovchinnikov state above the Pauli limit as a cause. This is also consistent with the observation of a magnetic field driven phase transition in the thermodynamic quantities within the superconducting state near the Pauli limit.
Emotional Responses And Perceived Stressors Of Frontline Medical Staffs In Case Of Addis Ababa Covid-19 Treatment Centers And Obstetrics Emergency And Abortion Care Clinic, Ethiopia; Analytical Cross Sectional Study
Authors: Kebede Mebratu , Demissie Dereje , Guddu Dessalegn , Haile Michael , Bitew Zebenay , Muleta Mahteme 
Published: December 2020

Abstract
Background: the rapid spread of COVID-19, its lethality in severe cases and the absence of specific medicine poses a huge threat to human life and health, as well as huge impact on the mental health. Facing this critical situation, health care workers on the front line who are directly involved in the diagnosis, treatment, and care of patients with COVID-19 are at risk of developing psychological distress and other mental health symptoms including emotional disturbance.Objective: the aim of this study will be to assess the current state of emotional responses and perceived stressors of frontline medical staffs in case of Addis Ababa COVID-19 Treatment Centers and obstetrics emergency and abortion care, Ethiopia 2020Methods: Hospital based comparative cross-section study design was conducted by using self-administered questionnaire survey from June 1st to 30th of 2020 among 133 and 266 frontline medical staffs from obstetric emergency and abortion care clinic and COVID-19 treatment centers respectively. The data were collected after getting written consent from each participant and it entered into the computer using Epi-data version 7, then exported to SPSS version 20 for further analysis. Descriptive analysis was done using frequencies & percent. All independent determinants with P-value <0.05 were used to identify important predictors of emotional responses and perceived stressors. Result; A total of 399 frontline medical staffs were included in the study. The mean age of the respondents of those who were working in obstetrics emergency and abortion care clinic was 27.47 (SD=3.46) years and it was 28.12 (SD=4.09) years for the other groups. This study revealed that, 72.9% and 5.6% of the study participant from obstetrics emergency and abortion clinic and COVID-19 treatment centers had a positive emotional response, respectively. Factors such as having a low level of motivational factors (AOR 2.78, 95% CI (1.13, 6.84)), being a nurse (AOR 10.53, 95% CI (1.31, 85.26)) and working at triage (AOR 8.61, 95% CI (1.15, 64.81))) had statistically significant association with negative emotional response.Conclusion; The current study revealed that a high proportion of front line a negative emotional responses had negative emotional response. Further, almost all of the medical staffs working in COVID-19 treatment centers and at obstetrics emergency and abortion care unit had perceived the outbreak related stressors. So, providing comprehensive psychological support is warranted for health care providers working in such kinds of department or units.
Study on the relationship of depression, anxiety, lifestyle and eating habits to the severity of reflux esophagitis
Authors: Wang Rongxin , Wang Jing , Hu Shuiqing 
Published: December 2020

Abstract
Background: The etiology of reflux esophagitis (RE) is multi-factorial. This study aims to analyze the risk factors of the RE, its severity and further explore the impact of anxiety and depression on the patients' symptoms and quality of life. Methods: From September 2016 to February 2018, a total of 689 subjects at Xuanwu Hospital Capital Medical University participated in the survey. They were divided into RE group (patients diagnosed as RE on gastroscopy, n = 361) and control group (healthy individuals without heartburn, regurgitation and other typical symptoms, n = 328). The survey included general demographic data, lifestyle habits, eating habits, GERD questionnaire (GerdQ), PHQ-9 depression scale and GAD-7 anxiety scale. Results: The mean age and sex ratio of the two groups were similar. Multivariate logistic regression analysis showed that low education level, drinking strong tea, preference for sweets and acidic foods, overeating, short interval between dinner and sleep, anxiety, depression, sleeping on low pillow and constipation were independent risk factors for RE (p< 0.05). Ordinal logistic regression analysis showed positive correlation between sleeping on low pillow and the severity of RE (p=0.024). Depression had positive correlation with the severity of symptoms (rs=0.375，P<0.001) and the quality of life (rs=0.306，P<0.001), whereas anxiety had no such association. Conclusions: Many lifestyle and eating habits are independent risk factors for the development of RE. Sleeping on low pillow was positively correlated with the severity of RE. Depression was positively related to the severity of symptoms and the quality of life.
MYPT1 reduction is a novel pathogenic factor of erectile dysfunction
Authors: Zhao Wei , Sun Jie , Yao Liang-Yu , Li Ye-Qiong , Chen Cai-Ping , Zhou Yu-Wei , Chen Xin , Tao Tao , Wei Li-Sha , Zheng Yan-Yan , Ge Xie , Li Chao-Jun , Xin Zhong-Cheng , Pan Yang , He Wei-Qi , Zhang Xue-Na , Yao Bing , Zhu Min-Sheng 
Published: December 2020

Abstract
Erectile dysfunction (ED) is closely associated with smooth muscle dysfunction, but its underlying mechanisms remains incompletely understood. We here reported that the reduced expression of myosin phosphatase target subunit 1 (MYPT1), the main regulatory unit of myosin light chain phosphatase, was critical for the development of vasculogenic ED. Male MYPT1 knockout mice had reduced fertility and the penises displayed abolished erections as evidenced by reduced intracavernous pressure (ICP). The penile smooth muscles of the knockout mice displayed enhanced contractility and resistant relaxation. We further identified a natural compound lotusine that increased the MYPT1 expression by inhibiting SIAH1/2 E3 ligases-mediated protein degradation. This compound sufficiently restored the ICP and improved histological characters of the penile artery of Mypt1 haploinsufficiency mice. In diabetic ED mice (db/db), the decreased expression of MYPT1 was measured, and ICP was restored by lotusine treatment. We conclude that the reduction of MYPT1 is the major pathogenic factor of vasculogenic ED. The restoration of MYPT1 by lotusine improved the function of injured penile smooth muscles, and could be a novel strategy for ED therapy.
Regulation of Arabidopsis photoreceptor CRY2 by two distinct E3 ubiquitin ligases
Authors: Chen Yadi , Hu Xiaohua , Liu Siyuan , Su Tiantian , Huang Hsiaochi , Ren Huibo , Gao Zhensheng , Wang Xu , Lin Deshu , Wang Qin , Lin Chentao 
Published: December 2020

Abstract
Cryptochromes (CRYs) are photoreceptors or components of the molecular clock in various evolutionary lineages, and they are commonly regulated by polyubiquitination and proteolysis. Multiple E3 ubiquitin ligases regulate CRYs in animal models, and previous genetics study also suggest existence of multiple E3 ubiquitin ligases for plant CRYs. However, only one E3 ligase, Cul4COP1-SPAs, has been reported for plant CRYs so far. Here we show that Cul3LRBs is the second E3 ligase of CRY2 in Arabidopsis. We demonstrated the blue light-specific and CRY-dependent activity of LRBs (Light-Response Bric-a-Brack/Tramtrack/Broad 1, 2 & 3) in blue-light regulation of hypocotyl elongation. LRBs physically interact with photoexcited and phosphorylated CRY2 to facilitate polyubiquitination and degradation of CRY2 in response to blue light. We propose that Cul4COP1-SPAs and Cul3LRBs E3 ligases interact with CRY2 via different structure elements to regulate the abundance of CRY2 photoreceptor under different light conditions, facilitating optimal photoresponses of plants grown in nature.
Using Deep Learning Models to Analyze The Cerebral Edema Complication Caused by Radiotherapy in Patients With Intracranial Tumor
Authors: Chao Pei-Ju , Chang Liyun , Kang Chen-Lin , Lin Chin-Hsueh , Shieh Chin-Shiuh , Wu Jia-Ming , Tseng Chin-Dar , Tsai I-Hsing , Hsu Hsuan-Chih , Huang Yu-Jie , Lee Tsair-Fwu 
Published: December 2020

Abstract
Using deep learning models to analyze patients with intracranial tumors, to study the image segmentation and standard results by clinical depiction complications of cerebral edema after receiving radiotherapy. In this study, patients with intracranial tumors receiving computer knife (CyberKnife M6) stereotactic radiosurgery were followed using the treatment planning system (MultiPlan 5.1.3) to obtain before-treatment and four-month follow-up images of patients. Images were preprocessed using images of cerebral edema complications (22 images, T2-flair) after treatment. The image interpolation was increased to 109 images and depicted as a standard image (ground truth segmentation, GTS) by the clinician. The TensorFlow platform was used as the core architecture for training neural networks. Supervised learning was used to build labels for the cerebral edema dataset by using Labelme, Mask region-based convolutional neural networks (R-CNN), and region growing algorithms. The three evaluation coefficients DICE, Jaccard (intersection over union, IoU), and volumetric overlap error (VOE) were used to analyze and calculate the algorithms in the image collection for cerebral edema image segmentation and the standard as described by the oncologists. When DICE and IoU indices were 1, and the VOE index was 0, the results were similar to those described by the clinician. The study found using the Mask R-CNN model in the segmentation of cerebral edema, the DICE index was 0.88, the IoU index was 0.79, and the VOE index was 2.0. The DICE, IoU, and VOE indices using region growing were 0.77, 0.64, and 3.2, respectively. Using the evaluated index, the Mask R-CNN model had the best segmentation effect, the volume of cerebral edema that most closely described the patient by the clinician. This study analyzes the situation of patients with intracranial tumors following radiotherapy after four months of follow-up to check for radiation-induced cerebral edema, using two methods to segment medical images. Among them, the instance segmentation method based on the deep learning Mask R-CNN has obtained the best results compared to the standard of the clinician in terms of describing the complications of cerebral edema. This method can be implemented in the clinical workflow in the future to achieve good complication segmentation and provide clinical evaluation and guidance suggestions.
Preliminary Estimates of Change in Physical Function After Targeted Kyphosis Exercise Intervention in Older Adults With Low Function: A Secondary Analysis of The SCOR Trial
Authors: Gladin Amy , Katzman Wendy , Fukuoka Yoshimi , Parimi Neeta , Wong Shirley , Lane Nancy 
Published: December 2020

Abstract
Background: Hyperkyphosis is common in older adults and associated with low physical function and reduced health related quality of life (HrQol). Improved kyphosis has been previously established in kyphosis-targeted interventions in randomized controlled trials in older adults with hyperkyphosis however evidence for improved physical function is conflicting. Few studies have investigated change in physical function after a targeted kyphosis intervention in older adults with low physical function. The primary aim in this descriptive study was to explore change in physical function after a progressive high intensity 3-month targeted kyphosis exercise and posture training intervention in older adults with low physical function and hyperkyphosis. Secondary aims were to explore change in HrQol, spinal strength and spinal curvature, and adherence and safety of the intervention in older adults with low physical function and hyperkyphosis.Methods: In this secondary analysis of the Specialized Center of Research (SCOR) Kyphosis randomized trial, 101 community dwelling older men and women with hyperkyphosis who completed the intervention were divided into a low function group (LFG) and high function group (HFG). Baseline characteristics were compared between LFG and HFG. Physical function, HrQol, spinal strength and spinal curvature (kyphosis and lordosis) pre/post intervention change scores were explored within and between groups. Adherence and adverse events were examined in the LFG and HFG. Results:Twenty-six (26%) older adults were LFG, mean SPPB 9.62 (SD=1.17) points. At baseline, the LFG was older than HFG (p=0.005), endorsed more pain, (p=0.060), had worse physical function and HrQol (p≤0.001), and comparable kyphosis (p=0.640). SPPB increased 0.62 (95% CI: -0.20 to 1.44) points in the LFG and decreased 0.04 (95%CI: -0.28 to 0.19) points in the HFG, p=0.020. Gait speed improved 0.04 (95%CI: -0.02 to 0.10) m/s in the LFG. Kyphosis improved equally in both groups. Adherence was similar and there were no adverse events in the LFG or HFG. Conclusions:Older adults with low physical function and hyperkyphosis may improve physical function after a kyphosis targeted intervention. Older adults with low physical function may safely participate in targeted high-intensity kyphosis exercise and posture training. This observation needs to be confirmed in larger adequately powered studies. Clinicaltrials.gov dentifier: NCT01766674
Unified theory for light-induced halide segregation in mixed halide perovskites
Authors: Chen Zehua , Brocks Geert , Tao Shuxia , Bobbert Peter 
Published: December 2020

Abstract
Mixed halide perovskites that are thermodynamically stable in the dark demix under illumination. This is problematic for their application in solar cells. We present a unified thermodynamic theory for this light-induced halide segregation that is based on a free energy lowering of photocarriers funnelling to a nucleated phase with different halide composition and lower band gap than the parent phase. We apply the theory to a sequence of mixed iodine-bromine perovskites. The spinodals separating metastable and unstable regions in the composition-temperature phase diagrams only slightly change under illumination, while light-induced binodals separating stable and metastable regions appear signalling the nucleation of a low-band gap iodine-rich phase. We find that the threshold photocarrier density for halide segregation is governed by the band gap difference of the parent and iodine-rich phase. Partial replacement of organic cations by cesium reduces this difference and therefore has a stabilizing effect.
Prevalence of Haemarthrosis and Clinical Impact on The Musculoskeletal System in People With Haemophilia in The United Kingdom; Evaluation of UK National Haemophilia Database and Haemtrack Patient Reported Data.
Authors: Wilkins Richard , Stephensen David , Siddle Heidi , Scott Martin , Xiang Hua , Horn Elizabeth , Palmer Ben , Chapman Graham , Richards Michael , Walwyn Rebecca , Redmond Anthony 
Published: December 2020

Abstract
Introduction; Severe haemophilia is a rare x-linked recessive genetic disorder characterised by bleeding into soft tissue and joints. Haemarthrosis is the most common bleeding manifestation in haemophilia, leading to haemarthropathy. The ankle joint has been reported as the most common site of bleeding, but it is unclear whether the ankle is most affected in terms of joint health status. Aims; To determine the prevalence of joint bleeding and musculoskeletal health status at an individual joint level of children and adults with severe haemophilia A and B without a current inhibitor.Methods; Application was made to the National Haemophilia Database (NHD) with a request for the following; Haemtrack patient reported treatment record and Haemophilia Joint Health Scores (HJHS) in children (<18y) and adults (≥18y) with severe haemophilia A (HA) and B (HB) (FVIII/FIX, <0.01 iu/ml) without a current inhibitor. Data were collated and reported for 1st January to 31st December 2018.Results; 2238 cases were identified, of which 273 were Haemtrack compliant with contemporaneous HJHS. The median (IQR) age of children was 10 (6-13) and adults 40 (29-50) years. Haemarthrosis prevalence in HA/HB children was 33% and 47%, respectively and 60% and 42%, respectively, in adults. The most common haemarthrosis site in children was the knee in HA and ankle in HB. In adults, the incidence of haemarthrosis at the ankles and elbows was equal. Median (IQR) total HJHS in HA/HB children were 0 (0;0). In adults with HA/HB, HJHS were 18 (6; 31) and 11 (5; 24), respectively. In adults with HA/HB, mean (SD) ankle HJHS of 3.8 (4.1) and median 4.0 (0.0; 8.0) were higher than the knee (mean 2.9 (4.1) and median 1.0 (0.0; 5.0) and elbow (mean 3.3 (4.1) and median 1.0 (0.0; 7.0) joints.Conclusion; During 2018, NHD prevalence data of haemarthrosis indicate only two-thirds of children and one-third of adults from a UK cohort compliant with prophylaxis were bleed free. HJHS of zero in children suggests joint health status is either unaffected during childhood or undetected by HJHS. In adults, higher HJHS are reported for the ankles indicating worse joint health.
Characteristics of Landscape Change Patterns at Three Distinct Stages of the Construction and Operation of the Three Gorges Reservoir From 1986 to 2017
Authors: Li Ruikang , Li Yangbing , Li Bo 
Published: December 2020

Abstract
Analyses of landscape change patterns that are based on elevation and slope can not only provide reasonable interpretations of landscape patterns but can also help to reveal evolutionary laws. As a complex geographical unit, the ecosystem environment in the middle reach of the Yangtze River has experienced great changes due to the construction of the Three Gorges Reservoir (TGR) and its associated human activities. Here, based mainly on a digital elevation model (DEM) and remotely sensed images from 1986, 2000, 2010, and 2017 and by using GIS technology, buffer analysis, landscape element change and landscape pattern indices, the spatial and temporal evolution characteristics of different elevations, slopes, and buffer landscape types were analyzed in a typical watershed, as well as an evolutionary model of the landscape pattern. The results indicated that (1) the landscape elements along the land classification and buffer zone that were influenced by the TGR construction have undergone a phased change, with the period 2000-2010 being the most dramatic period of landscape evolution during the impoundment period; (2) landscape type shifts from human-dominated farmland to nature-driven forestland and shrub-land as elevations, slopes and buffer distances increased. The landscape has shifted from diversity to relative homogeneity. (3) land types and buffer zones have exhibited a significant effect on the landscape pattern index, which is reflected in the differences in landscape type indices for spatial extension and temporal characteristics. The results of this study illustrate the pronounced effect of the TGR on landscape patterns, and these findings will elucidate the scientific basis and provide a reference for sustainable land resource management in the study region.