bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and Characterization of Aldehyde Oxidase 5 in the Pheromone Gland of the Silkworm (Lepidoptera: Bombycidae)
Authors: Zhang Yandi , Yang Yu , Shen Guanwang , Mao Xueqin , Jiao Mengyao , Lin Ying 
Published: October 2020

AbstractAldehyde oxidases (AOXs) are a subfamily of cytosolic molybdo-flavoenzymes that play critical roles in the detoxification and degradation of chemicals. Active AOXs, such as AOX1 and AOX2, have been identified and functionally analyzed in insect antennae but are rarely reported in other tissues. This is the first study to isolate and characterize the cDNA that encodes aldehyde oxidase 5 (BmAOX5) in the pheromone gland (PG) of the silkworm, Bombyx mori. The size of BmAOX5 cDNA is 3,741 nucleotides and includes an open reading frame, which encodes a protein of 1,246 amino acid residues. The theoretical molecular weight and isoelectric point of BmAOX5 are approximately 138 kDa and 5.58, respectively. BmAOX5 shares a similar primary structure with BmAOX1 and BmAOX2, containing two [2Fe-2S] redox centers, a FAD-binding domain, and a molybdenum cofactor (MoCo)-binding domain. RT–PCR revealed BmAOX5 to be particularly highly expressed in the PG (including ovipositor) of the female silkworm moth, and the expression was further confirmed by in situ hybridization, AOX activity staining, and anti-BmAOX5 western blotting. Further, BmAOX5 was shown to metabolize aromatic aldehydes, such as benzaldehyde, salicylaldehyde, and vanillic aldehyde, and fatty aldehydes, such as heptaldehyde and propionaldehyde. The maximum reaction rate (Vmax) of benzaldehyde as substrate was 21 mU and Km was 1.745 mmol/liter. These results suggested that BmAOX5 in the PG could metabolize aldehydes in the cytoplasm for detoxification or participate in the degradation of aldehyde pheromone substances and odorant compounds to identify mating partners and locate suitable spawning sites.
Estimations of Fine-Scale Species Distributions of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Eastern Florida
Authors: Hopperstad Kristen A , Sallam Mohamed F , Reiskind Michael H 
Published: October 2020

Abstract
Many species distribution maps indicate the ranges of Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) overlap in Florida despite the well-documented range reduction of Ae. aegypti. Within the last 30 yr, competitive displacement of Ae. aegypti by Ae. albopictus has resulted in partial spatial segregation of the two species, with Ae. aegypti persisting primarily in urban refugia. We modeled fine-scale distributions of both species, with the goal of capturing the outcome of interspecific competition across space by building habitat suitability maps. We empirically parameterized models by sampling 59 sites in south and central Florida over time and incorporated climatic, landscape, and human population data to identify predictors of habitat suitability for both species. Our results show human density, precipitation, and urban land cover drive Ae. aegypti habitat suitability, compared with exclusively climatic variables driving Ae. albopictus habitat suitability. Remotely sensed variables (macrohabitat) were more predictive than locally collected metrics (microhabitat), although recorded minimum daily temperature showed significant, inverse relationships with both species. We detected minor Aedes habitat segregation; some periurban areas that were highly suitable for Ae. albopictus were unsuitable for Ae. aegypti. Fine-scale empirical models like those presented here have the potential for precise risk assessment and the improvement of operational applications to control container-breeding Aedes mosquitoes.
Anticompetitive Effects in EU Competition Law
Authors: Ibáñez Colomo Pablo 
Published: October 2020

AbstractThis article examines the meaning and scope of the notion of anticompetitive effects in EU competition law. It does so by bringing together several strands of the case law (and this across all provisions, namely Articles 101 and 102 TFEU and merger control). The analysis is structured around a framework that considers the main variables that shape the notion in practice: the time variable (actual or potential effects); the dimensions of competition and the counterfactual; the meaning of effects and the probability threshold (plausibility, likelihood, certainty). The exercise shows that it is possible to discern a concrete meaning to the notion of anticompetitive effects. Some central questions, including the role and operation of the counterfactual and the threshold of effects, have already been answered by the Court of Justice. In particular, it has long been clear that anticompetitive effects amount to more than a mere competitive disadvantage and/or a limitation of a firm’s freedom of action. The impact on equally efficient firms’ ability and/or incentive to compete would need to be established. At the same time, some open questions and some potential areas of friction (relating, inter alia, to stakeholders’ tendency to conflate appreciability and effects) remain. These are also discussed.
Multisystem Inflammatory Syndrome in Children Associated With Severe Acute Respiratory Syndrome Coronavirus-2 in an 8-Week-Old Infant
Authors: Orlanski-Meyer Esther , Yogev Dotan , Auerbach Adi , Megged Orli , Glikman Daniel , Hashkes Philip J , Bar-Meir Maskit 
Published: October 2020

Abstract
We describe an 8-week-old infant with severe gastrointestinal symptoms, significant hypoalbuminemia, and mild carditis following asymptomatic infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The infant’s symptoms, including their temporal appearance, were consistent with multisystem inflammatory syndrome in children (MIS-C). A unique finding on colonic histology which may shed light on the pathogenesis of MIS-C was identified. The patient improved significantly following several anti-inflammatory treatments. The lag between the presentation of MIS-C and initial SARS-CoV-2 exposure, which may often be asymptomatic, together with the young age of our patient, makes this a challenging diagnosis. Clinicians should be aware of this entity, even in the neonatal and infantile age groups, to facilitate timely identification and treatment.
A rare case of lumbosacrococcygeal mass in newborn: a human tail
Authors: Daib Aida , Saadi Cyrine , Rabiaa Ben Abdallah R , Barguellil Marwa , Hellal Youssef , Trabelsi F , Jabloun A , Kaabar N 
Published: October 2020

Abstract
Caudal cutaneous appendage is a rare condition. According to association with underlying spinal dysraphism, it can be classified into true or pseudotails. Management and prognosis depends closely on spinal anomaly. Fewer than 40 cases of true tail were reported. We describe a rare case of true tail in a newborn explored and operated in our unity.
Struma ovarii: two case reports of a rare teratoma of the ovary
Authors: Rockson Obed , Kora Christine , Ramdani Abdelbassir , Basma Aabdi , Bouhout Tariq , Serji Badr , El Harroudi Tijani 
Published: October 2020

Abstract
Struma ovarii is an extremely rare type of ovarian teratoma distinguished by the unusual presence of thyroid tissue. It is usually a benign condition; however, malignant transformation is sometimes detected. The diagnosis relies on histopathological examination and is infrequently made on routine investigations. We report two cases of struma ovarii: one benign and the other malignant. The first case involved a 27-year-old woman who underwent a right ovarian cystectomy for an incidental ultrasonographic finding of a solid right adnexal mass. The diagnosis of a benign struma was made after histopathological study. The second case involved a 68-year-old woman who underwent a right salpingo-oophorectomy for a right ovarian bulky mass and the histopathological diagnosis was consistent with that of a malignant struma. We examine the challenges involved in the diagnosis and management of this rare entity.
Fluorescent protein-based imaging and tissue-specific RNA-seq analysis of Arabidopsis hydathodes
Authors: Yagi Hiroki , Nagano Atsushi J , Kim Jaewook , Tamura Kentaro , Mochizuki Nobuyoshi , Nagatani Akira , Matsushita Tomonao , Shimada Tomoo 
Published: October 2020

AbstractHydathodes are typically found at leaf teeth in vascular plants and are involved in water release to the outside. Although morphological and physiological analysis of hydathodes has been performed in various plants, little is known about the genes involved in hydathode function. In this study, we performed fluorescent protein-based imaging and tissue-specific RNA-seq analysis in Arabidopsis hydathodes. We used the enhancer trap line E325, which has been reported to express green fluorescent protein (GFP) at its hydathodes. We found that E325-GFP was expressed in small cells found inside the hydathodes (named E cells) that were distributed between the water pores and xylem ends. No fluorescence of the phloem markers pSUC2:GFP and pSEOR1:SEOR1-YFP was observed in the hydathodes. These observations indicate that Arabidopsis hydathodes are composed of three major components: water pores, xylem ends, and E cells. In addition, we performed transcriptome analysis of the hydathode using the E325-GFP line. Microsamples were collected from GFP-positive or -negative regions of E325 leaf margins with a needle-based device (~130 µm in diameter). RNA-seq was performed with each single microsample using a high-throughput library preparation method called Lasy-Seq. We identified 72 differentially expressed genes. Among them, 68 genes showed significantly higher and four genes showed significantly lower expression in the hydathode. Our results provide new insights into the molecular basis for hydathode physiology and development.
ABI5 regulates ABA-induced anthocyanin biosynthesis by modulating the MYB1-bHLH3 complex in apple
Authors: An Jian-Ping , Zhang Xiao-Wei , Liu Ya-Jing , Wang Xiao-Fei , You Chun-Xiang , Hao Yu-Jin 
Published: October 2020

AbstractAbscisic acid (ABA) induces anthocyanin biosynthesis in many plant species. However, the molecular mechanism of ABA-regulated anthocyanin biosynthesis remains unclear. As a crucial regulator of ABA signaling, ABSCISIC ACID-INSENSITIVE5 (ABI5) is involved in many aspects of plant growth and development, yet its regulation of anthocyanin biosynthesis has not been elucidated. In this study, we found that MdABI5, the apple homolog of Arabidopsis ABI5, positively regulated ABA-induced anthocyanin biosynthesis. A series of biochemical tests showed that MdABI5 specifically interacts with basic helix-loop-helix 3 (MdbHLH3), a positive regulator of anthocyanin biosynthesis. MdABI5 enhanced the binding of MdbHLH3 to its target genes dihydroflavonol 4-reductase (MdDFR) and UDP flavonoid glucosyl transferase (MdUF3GT). In addition, MdABI5 directly bound to the promoter of MdbHLH3 to activate its expression. Moreover, MdABI5 enhanced ABA-promoted interaction between MdMYB1 and MdbHLH3. Finally, antisense suppression of MdbHLH3 significantly reduced anthocyanin biosynthesis promoted by MdABI5, indicating that MdABI5-promoted anthocyanin biosynthesis was dependent on MdbHLH3. Taken together, our data suggest that MdABI5 plays a positive role in ABA-induced anthocyanin biosynthesis by modulating the MdbHLH3-MdMYB1 complex. Our work broadens the regulatory network of ABA-mediated anthocyanin biosynthesis, providing new insights to further study the transcriptional regulatory mechanisms behind this process.
Potential Use of Antigen-Based Rapid Test for SARS-CoV-2 in Respiratory Specimens in Low-Resource Settings in Egypt for Symptomatic Patients and High-Risk Contacts
Authors: Abdelrazik Abeer Mohamed , Elshafie Shahira Morsy , Abdelaziz Hossam M 
Published: October 2020

Abstract

Objective
Because of the rapidly emerging SARS-CoV-2 pandemic and its wide public health challenges, rapid diagnosis is essential to decrease the spread. Antigen-based rapid detection tests are available; however, insufficient data about their performance are available.

Methods
The lateral-flow immunochromatographic BIOCREDIT COVID-19 antigen test was evaluated using nasopharyngeal swabs in a viral transport medium from patients with confirmed infection, contacts, and exposed healthcare professionals at Fayoum University Hospital in Egypt. Test performance was determined in comparison to the SARS-CoV-2 real-time reverse-transcription polymerase chain reaction (RT-PCR) test.

Results
Three hundred ten specimens from 3 categories—patients with confirmed diagnoses of COVID-19, contacts, and exposed healthcare professionals—were included; 188 specimens were RT-PCR-positive, from which 81 were detected by rapid antigen test. Overall sensitivity was 43.1%. Sensitivity was significantly higher in specimens with high viral loads.

Conclusion
Poor sensitivity of the BIOCREDIT COVID-19 test does not permit its use for diagnosis, and it can only be used in conjunction with RT-PCR for screening.
Unmet Healthcare Needs and Associated Factors Among Korean Enlisted Soldiers
Authors: Bae Eunkyoung , Park Jeongok , Jung Eunyoung 
Published: October 2020

ABSTRACTIntroductionThe purpose of this study was to explore the rate of unmet healthcare needs among Korean enlisted soldiers and to examine the factors associated with unmet healthcare needs, based on Andersen’s Health Service Behavior Model.MethodThis study employed secondary data analysis using the dataset of the military health survey for two years, 2014 and 2015, conducted by the Republic of Korea School of Military Medicine. Of all military health survey participants, 3,636 enlisted soldiers who answered the question “In the last year, have you ever been unable to go to a hospital or a clinic when you wanted to go to a hospital or a clinic?”—the dependent variable in this study—were included in this study.ResultThe rate of unmet healthcare needs among Korean enlisted soldiers was 23.7% (n = 860) in the current study. Among the individual characteristics, on-duty stress (Odds ratio (OR) = 1.59, 95% Confidence Interval (95% CI): 1.25-2.03) and need for treatment by a mental health specialist (OR = 1.40, 95% Confidence Interval (CI): 1.00-1.95) were associated with unmet healthcare needs. Among the contextual characteristics, perception of injury-prevention effort (OR = 0.61, 95% CI: 0.52-0.71), support from superiors (OR = 0.81, 95% CI: 0.71-0.93), and possibility of accident or injury of military unit (OR = 0.73, 95% CI: 0.64-0.82) were significant factors associated with unmet healthcare needs. The subjective health condition which was a proxy of the health outcome was significantly associated with unmet healthcare needs (OR = 1.58, 95% CI: 1.13-2.22). The results indicate that the safety awareness of troops and social support of superiors were the contextual military characteristics associated with unmet healthcare needs of enlisted soldiers.ConclusionsThe strengthening of aspect of organizational culture, such as injury-prevention efforts at the military level, or support from superior officers, will contribute to a reduction of the unmet healthcare needs of Korean enlisted soldiers.
Pseudofolliculitis Barbae in the U.S. Military, a Review
Authors: Tshudy Michael T , Cho Sunghun 
Published: October 2020

ABSTRACT

Introduction
Pseudofolliculitis barbae (PFB), also known as ingrown hairs commonly results from adherence to military grooming standards in those who have curly facial hair. Many patients respond to specific grooming techniques or topical medications but severe cases often warrant restrictions on shaving or laser therapy. The treatment of PFB is challenged not only by grooming standards but also evolving readiness requirements. More recently, the Navy discontinued permanent restrictions on shaving because of concerns for poor gas mask fitting. The goal of this review is to outline the history of PFB in the military, describe current policies, and offer a more uniform approach to treating PFB in the military. We also discuss challenges that surround the management of PFB in the Armed Forces.

Materials and Methods
We conducted a systematic review of the literature utilizing PubMed to identify both current and past management and discussion of PFB in both civilian and military settings. We also performed an internet search to identify pertinent military regulations and history of PFB within the Armed Forces. A query of TRICARE, the health care program for the U.S. Department of Defense Military Heath System, was also performed to assess civilian-referred laser treatment for Active Duty service members.

Results
PFB has a long history in the military dating back to World War I, when shaving was enforced not only to ensure good personal hygiene and to foster good order and discipline but also to ensure gas masks would seal in the event of a chemical attack. The management of PFB has presented a challenge in the military and even led to a social uproar in the 1970s. Policy changes in the military regarding shaving limitations, or profiles, have historically influenced how PFB is managed, but the basic tenets remain the same. Grooming techniques and topical medications can be effective in treating mild-to-moderate disease, but more severe cases of PFB respond best to laser therapy. Limitations on shaving remain an important part of management, especially during flares of the disease and while initiating therapy. Long-term shaving profiles may be needed for treatment-resistant cases. The impact of the Navy’s recent policy on PFB is also discussed.

Conclusions
Pseudofolliculitis Barbae is a prevalent skin disease in the Armed Forces. A better understanding of the disease and patients’ individual needs by the health care provider and commander is paramount.
Soldiers’ Perception of Combat Ration Use During Arctic Training: A Qualitative Study
Authors: Lavergne Florence V , Prud’homme Denis , Giroux Isabelle 
Published: October 2020

ABSTRACTIntroductionSoldiers work in various extreme environments, including the High Arctic, where energy requirements are increased compared with temperate climates. Soldiers often do not reach their energy needs with combat rations and face additional challenges to feeding in the Artic, which can hinder the performance. The purpose of this study is to document soldiers’ perception about individual, dietary, and environmental factors influencing intake of combat rations during Arctic field training.Materials and MethodsThis qualitative phenomenological study included in-depth semi-structured individual interviews with 16 soldiers of the Canadian Armed Forces participating in the Arctic Operations Advisor training in Yellowknife (Northwest Territories) and Resolute Bay (Nunavut) from January to March 2019. Interviews were audio-recorded, transcribed verbatim, and then coded using a directed content analysis approach. Data were analyzed with NVivo qualitative data analysis software.ResultsFive themes related to the individual (personal preferences; mood and morale), the diet (water availability; food variety), or the environment (meal preparation time) were identified. A sixth theme found was related to the diet and the environment (food/water temperature). Soldiers explained food and water were frozen, thus limiting water availability and greatly increasing meal preparation time. Food variety was deemed adequate by some, but others preferred more options. Individual food preferences and soldier mood and team morale could be barriers or facilitators to intake. Overall, the complexity of combat ration intake in the Arctic stemmed from the interaction of factors.ConclusionsVarious factors related to the individual, diet, and environment were found to influence intake of combat rations by participating soldiers during Arctic training. Reducing barriers to combat ration consumption by enhancing operational suitability of rations for the Arctic environment could promote dietary intake. Bearing in mind many interrelated factors influenced intake of soldiers, the military would benefit from further assessing which challenges related to intake in the field could be addressed.
Bruxism and Stress Among Veterans With Gulf War Illness
Authors: Carter Kandis , McKenzie Carly T 
Published: October 2020

ABSTRACT

Introduction
This study explores perceived stress and experience with bruxism among veterans with Gulf War Illness (GWI). Stress may manifest physically as bruxism, a parafunctional oral activity that consists of teeth grinding and/or clenching.

Materials and Methods
An online survey of GWI veterans (n = 28, 27.7% response rate) assessed perceived general stress and self-reported behaviors, symptoms, and outcomes associated with bruxism. Survey questions also collected basic demographic data and past military experience. The appropriate Institutional Review Board approved this study (IRB-300001376). Statistical analyses utilized both analysis of variance and linear regression techniques in addition to descriptive statistics.

Results
This sample of GWI veterans reported higher levels of perceived stress (M = 20.2, SD = 7.0) than general population males (M = 12.1, SD = 5.9). A majority of GWI veterans reported both grinding (77.8%) and clenching (85.2%) teeth on a weekly or daily basis. Grinding frequency did not predict perceived stress scale values (F = 2.38, P = .11). Clenching frequency did significantly predict perceived stress scale values (F = 4.07, P = .03). Those who reported daily clenching had significantly higher perceived stress scores (M = 22.17, SD = 5.87) than did those who reported never clenching (M = 12.00, SD = 5.35). Length of military service did not significantly predict perceived stress or bruxism experience.

Conclusions
GWI veterans reported higher levels of perceived stress in comparison with that of general population males. Both the high frequency of teeth grinding and clenching in these patients is a potential physical manifestation of the high perceived stress levels reported. It is imperative that both military and civilian dentists and physicians are aware of the potential for increased stress and consequently bruxism in this patient population as it can have negative impacts on oral and mental health. Treatment of these patients can include but is not limited to behavior modification, stress reduction training, and the fabrication of mouth guards. The dental and medical implications of bruxism and stress in veterans with GWI should be further investigated.
Case Study: Acute Lumbar Paraspinal Compartment Syndrome in an Austere Military Environment
Authors: Allen Pete , Pugh Jackson , Blau Alexander 
Published: October 2020

ABSTRACT
The incidence of compartment syndrome of the lumbar paraspinal muscles is exceedingly rare. Approximately 24 hours following a high-intensity kettlebell swing workout, a 33-year-old Sailor presented to the medical department on board a forward deployed Wasp-class amphibious assault ship with increasing discomfort in his middle and lower back, and evidence of rhabdomyolysis. Discomfort quickly turned to unrelenting pain coupled with dorsal paresthesias and rigidity in the paraspinal muscles. He was taken emergently to the operating room, where his paraspinal muscles were released via fasciotomy. As a result of limited resources aboard the deployed ship, a negative pressure wound dressing was fashioned using the supplies available aboard the ship. Following 3 days of the negative pressure wound therapy, muscle bulging decreased substantially, and the skin was closed. After 4 weeks of physical therapy, he returned to full duty.