bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robust range of auditory periphery development, eye opening, and brain gene expression in Wistar rat pups that experience variation in maternal backgrounds
Authors: Qiu Jingyun , Singh Preethi , Pan Geng , de Paolis Annalisa , Champagne Frances A. , Liu Jia , Cardoso Luis , Rodríguez-Contreras Adrián 
Published: February 2020

ABSTRACTThe experience of variation in maternal licking and grooming (LG) is considered a critical influence in neurodevelopment related to stress and cognition, but little is known about its relationship to early sensory development. In this study, we used a maternal selection approach to test the hypothesis that differences in LG during the first week of life influence the timing of hearing onset in Wistar rat pups. We performed a range of tests, including auditory brainstem responses (ABR), tracking of eye opening (EO), micro-CT X-ray tomography, and qRT-PCR to monitor neurodevelopmental changes in the female and male progeny of low-LG and high-LG dams. Our results show that variation in maternal LG is not overtly associated with different timing of ABR onset and EO in the progeny. However, the data provide insight on the delay between hearing onset and EO, on key functional and structural properties that define hearing onset at the auditory periphery, and on changes in brain gene expression that include the first evidence that: a) the hypoxia-sensitive pathway is regulated in subcortical and cortical auditory brain regions before hearing onset, and b) implicates maternal LG in regulation of Bdnf signaling in auditory cortex after hearing onset. Altogether, these findings provide a baseline to evaluate how factors that severely disrupt the early maternal environment may affect the expression of robust developmental sensory programs.SIGNIFICANCE STATEMENTEarly life experience during sensitive developmental periods can induce long-term effects on the neurobiological development of the offspring. In the present work we tested the hypothesis that variation in maternal licking and grooming (LG) affects the timing of hearing onset in Wistar rat pups. To our surprise the results did not support the hypothesis. Instead, we found a robust range of early and late auditory development that was independent of maternal LG. Nevertheless, the study provides new findings on the delay between hearing onset and eye opening, on key functional and structural properties that define hearing onset at the auditory periphery, and the first evidence that a) the hypoxia-sensitive pathway is regulated in the central auditory system during the sensitive period before hearing onset, and b) maternal LG is implicated in regulation of Bdnf signaling during the sensitive period after hearing onset. These findings provide a baseline to evaluate how factors that severely disrupt the early maternal environment may affect the expression of robust developmental sensory programs.
Sphingolipids regulate the tethering stage of vacuole fusion by affecting membrane fluidity
Authors: Zhang Chi , Hurst Logan R. , Gokbayrak Zeynep D. , Calderin Jorge D. , Hrabak Michael R. , Balutowski Adam , Rivera-Kohr David A. , Kazmirchuk Thomas D.D. , Brett Christopher L. , Fratti Rutilio A. 
Published: February 2020

AbstractSphingolipids are essential in membrane trafficking and cellular homeostasis. Here, we show that sphingolipids containing very long-chain fatty acids (VLCFAs) promote robust homotypic vacuolar fusion inSaccharomyces cerevisiae. The elongase Elo3 adds two carbons to 24-carbon (C24) acyl chains to make C26 VLCFAs that are incorporated into sphingolipids. Vacuoles isolated fromelo3Δ cells had increased fluidity relative to the wild-type and were attenuated for fusion. Upon further testing we found that vesicle the tethering stage was affected aselo3Δ vacuole clusters contained fewer vesicles versus the WT. Vacuole tethering requires the interactions of late endosomal Rab GTPase Ypt7 and the HOPS tethering complex. Pulldown assays using GST-Ypt7 showed that HOPS fromelo3Δ vacuole extracts failed to bind Ypt7 while HOPS from WT extracts interacted with GST-Ypt7. Furthermore GFP-Ypt7 failed to localize at vertex microdomains ofelo3Δ vacuoles relative to the WT, whereas HOPS and regulatory lipids did accumulate at vertices. Finally, we found thatelo3Δ vacuoles had reduced V-ATPase. Together these data show that C26-VLCFA containing sphingolipids are important for Ytp7 function and vacuole homeostasis.
Long-read genome sequencing and assembly ofLeptopilina boulardi: a specialistDrosophilaparasitoid
Authors: Khan Shagufta , Sowpati Divya Tej , Srinivasan Arumugam , Soujanya Mamilla , Mishra Rakesh K 
Published: February 2020

ABSTRACTLeptopilina boulardi(Hymenoptera: Figitidae) is a specialist parasitoid ofDrosophila. TheDrosophila-Leptopilinasystem has emerged as a suitable model for understanding several aspects of host-parasitoid biology. However, a good quality genome of the wasp counterpart was lacking. Here, we report a whole-genome assembly ofL. boulardito bring it in the scope of the applied and fundamental research onDrosophilaparasitoids with access to epigenomics and genome editing tools. The 375Mb draft genome has an N50 of 275Kb with 6315 scaffolds >500bp and encompasses >95% complete BUSCOs. Using a combination ofab-initioand RNA-Seq based methods, 25259 protein-coding genes were predicted and 90% (22729) of them could be annotated with at least one function. We demonstrate the quality of the assembled genome by recapitulating the phylogenetic relationship ofL. boulardiwith other Hymenopterans. The key developmental regulators like Hox genes and sex determination genes are well conserved inL. boulardi, and so is the basic toolkit for epigenetic regulation. The search for epigenetic regulators has also revealed thatL. boulardigenome possesses DNMT1 (maintenance DNA methyltransferase), DNMT2 (tRNA methyltransferase) but lacks thede novoDNA methyltransferase (DNMT3). Also, the heterochromatin protein 1 family appears to have expanded as compared to other hymenopterans. The draft genome ofL. boulardi(Lb17) will expedite the research onDrosophilaparasitoids. This genome resource and early indication of epigenetic aspects in its specialization make it an interesting system to address a variety of questions on host-parasitoid biology.
Foucault's toolbox: use of Foucault's writings in LIS journal literature, 1990–2016
Authors: Dewey Scott Hamilton 
Published: February 2020

PurposeTo provide a close, detailed analysis of the frequency, nature, and depth of visible use of Michel Foucault's works by library and information science/studies (LIS) scholars.Design/methodology/approachThe study conducted extensive full-text searches in a large number of electronically available LIS journal databases to find citations of Foucault's works, then examined each cited article to evaluate the nature and depth of use.FindingsMost uses of Foucault are brief or in passing. In-depth explorations of Foucault's works are comparatively rare and relatively little-used by other LIS scholars. Yet the relatively brief uses of Foucault encompass a wide array of different topics spread across a wide spectrum of LIS journal literature.Research limitations/implicationsThe study was limited to articles from particular relatively prominent LIS journals. Results might vary if different journals or non-journal literature were studied. More sophisticated bibliometric techniques might reveal different relative performance among journals and might better test, confirm, or reject various patterns and relationships found here. Other research approaches, such as discourse analysis, social network analysis, or scholar interviews, might reveal patterns of use and influence not visible in this literature sample.Originality/valueThis intensive study of both quality and quantity of citations may challenge some existing assumptions regarding citation analysis, plus illuminating Foucault scholarship. It also indicates possible problems for future application of artificial intelligence (AI) approaches to similar depth-of-use studies.
Exploring the consequences of knowledge hiding: an agency theory perspective
Authors: Khoreva Violetta , Wechtler Heidi 
Published: February 2020

PurposeThe purpose of the study is to explore empirically the consequences of knowledge hiding at the individual level and from the knowledge hiding committers' perspective. Hence, in line with agency theory and prior literature on knowledge hiding, the study investigates the associations between different facets of knowledge hiding and individual-level job performance, as well as the mediating role of employee well-being in the associations.Design/methodology/approachStructural equation modeling was used to analyze multisource survey data from a sample of 214 employees and 34 immediate supervisors, in a professional services company in Finland.FindingsEvasive hiding was found to be negatively associated with in-role job performance and positively associated with innovative job performance. Playing dumb was found to be positively associated with in-role job performance. Finally, even though the association between rationalized hiding and innovative job performance was found to be positive, it was found to be of a smaller magnitude when employee well-being was taken into account.Practical implicationsForceful unhealthy competition and exploitative and workaholic cultures are discussed to reduce knowledge hiding behavior among employees and their negative consequences.Originality/valueThe study highlights the paradox of managing organizational knowledge. In line with agency theory, we advocate that while knowledge sharing is one of the major assets of organizational welfare from the organizational perspective, it may resonate with the employee's perspective. Consequently, unless employees' self-interest and organizational interests are aligned, the paradox of managing organizational knowledge arises, and the classic agency problem occurs.
Dynamic evolution and coordination mechanism of RSSC under the steady-state quality constraints
Authors: Li Jianfei , Sun Mengxia , Ren Li , Li Bei 
Published: February 2020

Purpose
The advent of the new retail era witnessed the consumers’ demand shift from on the traditional product quality to on the full supply chain service quality, and product service and service manufacturing is gradually taking shape. The purpose of this paper is to propose whether there is a “quality bridge” in the dynamic evolution process of retail service supply chain (RSSC) and discuss the system role, steady-state characteristics and dynamic evolution mechanism of service quality in this dynamic evolution process.

Design/methodology/approach
This paper proposes the dissipation system structure of the RSSC under the steady-state quality constraint, constructs a Markov chain model (MCM) for the evolution of the service quality of RSSC, and tests the objective existence of the steady-state distribution of the service quality by taking Chinese HJ retail enterprises as samples.

Findings
The research value of this study is summarized as follows. The research finds that the evolution of service quality of RSSC is a dynamic and non-linear growth process, which has significant characteristics of complex adaptability and steady-state convergence. The study finds that the quality evolution process of the RSSC is a steady-state convergence process, and there is a steady-state distribution of quality in its co-evolution, in which different process input levels have a significant positive impact on the stable level of quality state. The study finds that the steady state of quality plays a crucial role in the collaborative evolution of the RSSC, that is, when the service quality reaches a certain steady state distribution, the operating efficiency and profit level of the whole chain will show an “explosive” growth trend.

Originality/value
Quality bridge, an original concept in this paper, represents the role of quality steady-state in the operation of RSSC. Based on Markov chain and system simulation tools, this paper verifies the existence of steady-state service quality and its positive effect on the co-evolution and sustainable development of RSSC. When the service quality reaches a certain steady distribution, the operating efficiency and income level of the whole chain will show n trend of explosive growth.
Systematic research to overcome newly emerged multidrug‐resistant bacteria
Authors: Arakawa Yoshichika 
Published: February 2020

AbstractIn the 1980s, I found that the chromosomal β‐lactamase of Klebsiella pneumoniae LEN‐1 showed a very high similarity to the R‐plasmid‐mediated penicillinase TEM‐1 on the amino acid sequence level, and this strongly suggested the origination of TEM‐1 from the chromosomal penicillinases of K. pneumoniae or related bacteria. Moreover, the chromosomal K1 β‐lactamase (KOXY) of Klebsiella oxytoca was found to belong to the class A β‐lactamases that include LEN‐1 and TEM‐1, although KOXY can hydrolyze cefoperazone (CPZ) like the chromosomal AmpC‐type cephalosporinases of various Enterobacteriaceae that can hydrolyze several cephalosporins including CPZ. Furthermore, my collaborators and I found plural novel serine‐type β‐lactamases, such as MOX‐1, SHV‐24, TEM‐91, CTX‐M‐64, CMY‐9, CMY‐19, GES‐3, GES‐4, and TLA‐3, mediated by plasmids. Besides these serine‐type β‐lactamases, we also first identified exogenously acquired metallo‐β‐lactamases (MBLs), IMP‐1 and SMB‐1, in imipenem‐resistant Serratia marcescens, and the IMP‐1‐producing S. marcescens TN9106 became the index case for carbapenemase‐producing Enterobacteriaceae. I developed the sodium mercaptoacetic acid (SMA)‐disk test for the simple identification of MBL‐producing bacteria. We were also the first to identify a variety of plasmid‐mediated 16S ribosomal RNA methyltransferases, RmtA, RmtB, RmtC, and NpmA, from various Gram‐negative bacteria that showed very high levels of resistance to a wide range of aminoglycosides. Furthermore, we first found plasmid‐mediated quinolone efflux pump (QepA) and fosfomycin‐inactivating enzymes (FosA3 and FosK). We also first characterized penicillin reduced susceptible Streptococcus agalactiae, macrolide‐resistant Mycoplasma pneumoniae, as well as Campylobacter jejuni, and Helicobacter pylori, together with carbapenem‐resistant Haemophilus influenzae. We constructed a PCR‐based open reading frame typing method for rapid identification of Acinetobacter baumannii international clones.
Have R&D Spillovers Declined in the 21st Century?
Authors: Lucking Brian , Bloom Nicholas , Van Reenen John 
Published: February 2020

AbstractSlow growth over the last decade has prompted policy attention towards increasing R&D spending, often via the tax system. We examine the impact of R&D on firm performance, both by the firm's own investments and through positive (and negative) spillovers from other firms. We analyse panel data on US firms over the last three decades, and allow for time‐varying spillovers in both technology space (knowledge spillover) and product market space (product market rivalry). We show that the magnitude of R&D spillovers remains as large in the second decade of the 21st century as it was in the mid 1980s. Since the ratio of the social return to the private return to R&D is about four to one, this implies that there remains a strong case for public support of R&D. Positive spillovers appeared to temporarily increase in the 1995–2004 digital technology boom. We also show how these micro estimates relate to estimates from the endogenous growth literature and give some suggestions for future work.
Medical Labour Supply and the Production of Healthcare
Authors: Lee Tom , Propper Carol , Stoye George 
Published: February 2020

AbstractMedical labour markets are important because of their size and the importance of medical labour in the production of healthcare and in subsequent patient outcomes. We present a summary of important trends in the UK medical labour market, and we review the latest research on factors that determine medical labour supply and the impact of labour on patient outcomes. The topics examined include: the responsiveness of labour supply to changes in wages, regulation and other incentives; factors that determine the wide variation in physician practice and style; and the effect of teams and management quality on patient outcomes. This literature reveals that while labour supply is relatively unresponsive to changes in wages, medical personnel do react strongly to other incentives, even in the short run. This is likely to have consequences for the quality of care provided to patients. We set out a series of unanswered questions in the UK setting, including: the importance of non‐financial incentives in recruiting and retaining medical staff; how individuals can be incentivised to work in particular specialties and regions; and how medical teams can be best organised to improve care.
Fatal Aspiration of Barium Sulfate in a Case of Myasthenia Gravis: A Case Report and Review of Literature
Authors: Khartade Harshwardhan K. , Meshram Vikas P. , Tumram Nilesh K. , Parchake Manoj B. , Pathak Harish M. 
Published: February 2020

AbstractBarium contrast radiography is a usual procedure performed for detecting lesions of the digestive tract using barium sulfate on X‐ray irradiation. The aspiration of barium contrast is an uncommon but well‐documented complication occurring accidentally during examinations of the upper gastrointestinal system using contrast media. Certain conditions that affect the anatomical and functional integrity of the oropharynx and esophagus can be predisposing factors. Barium is an inert material that can cause symptoms varying from an asymptomatic mechanical obstruction to severe respiratory distress that can result in death when aspirated. Sudden death due to aspiration of barium is rarely reported in the literature. We report a case of a 50‐year‐old female who suddenly developed difficulty in breathing, followed by loss of consciousness during barium swallow procedure due to aspiration of barium contrast. She could not be revived and died within a few minutes of the episode. The predisposing factor was the weakness of the muscles of deglutition due to myasthenia gravis. We also reviewed the literature available on cases resulting from the aspiration of barium sulfate during the barium study procedures. We considered the factors like the age, indications, the distribution of barium in the lungs, clinical presentation, the period of survival, and cause of death. We came across only four cases of death within 24 h following the aspiration of barium. This case report and review of literature emphasize the importance of consideration of predisposing factors before conducting barium contrast radiography and also the possibility of sudden death.
Forecasting daily counts of patient presentations in Australian emergency departments using statistical models with time‐varying predictors
Authors: Duwalage Kalpani I , Burkett Ellen , White Gentry , Wong Andy , Thompson Mery H 
Published: February 2020

AbstractObjectiveThis research aimed to (i) assess the effects of time‐varying predictors (day of the week, month, year, holiday, temperature) on daily ED presentations and (ii) compare the accuracy of five methods for forecasting ED presentations, including four statistical methods and a machine learning approach.MethodsPredictors of ED presentations were assessed using generalised additive models (GAMs), generalised linear models, multiple linear regression models, seasonal autoregressive integrated moving average models and random forest. The accuracy of short‐term (14 days), mid‐term (30 days) and long‐term (365 days) forecasts were compared using two measures of forecasting error.ResultsThe data are the numbers of presentations to public hospital EDs in South‐East Queensland, Australia, from 2009 to 2015. ED presentations are largely affected by year of presentation, and to a lesser extent by month, day of the week and holidays. Maximum daily temperature is also a significant predictor of ED presentations. Of the four statistical models considered, the GAM had the greatest forecasting accuracy, and produced consistent and coherent forecasts, likely due to its flexibility in modelling complex time‐varying effects. The random forest machine learning approach had the lowest forecasting accuracy, likely due to overfitting the data.ConclusionsCalendar and temperature variables, not previously considered in the Australian literature, were found to significantly impact ED presentations. This study also demonstrates the potential of GAMs as a dual explanatory and forecasting method for the modelling, and more accurate prediction, of ED presentations.
Reconnecting with nature: an ecofeminist view of environmental management
Authors: Phillips Mary E. 
Published: February 2020

Key insightsCurrent conceptions of environmental management are limited and will not halt, let alone reverse, the disastrous impacts of capitalism on the planet. Ecofeminist philosophy offers a set of principles to challenge those conceptions and to build a different relationship with nature: first, by developing ecocentric connections where humanity is seen as part of the web of life; second, by revaluing epistemological frameworks to include what is currently denied by hyper‐rationalism; and, finally, by focusing on an ethic of care as a moral imperative and call for action.
“I was a homo thug, now I'm just homo”: Gay gang members’ desistance and persistence
Authors: Panfil Vanessa R. 
Published: February 2020

AbstractIn a growing body of research, the methods of and motivations for gang desistance are being investigated, spurred in part by concerns about the long‐term negative effects of gang membership. Despite recent calls for scholarship that is more inclusive of LGBTQ populations and attentive to issues of sexual identity, however, most gang research remains overwhelmingly heteronormative. In this study, I use in‐depth interviews with 48 self‐identified gay male gang members to explore how and why they have desisted from or persisted in their gangs, as well as explore how desistance or persistence has affected their self‐perceptions, lives, and activities. Because not all have left their gangs, I examine the markers in young men's narratives that signal shifts away from—but sometimes also toward—their gangs, as well as their zigzagging paths out of gang involvement. As gang structure and composition hold importance for their members’ experiences, I use a comparative approach by contrasting men in predominantly straight gangs with those in gay gangs. Set within a heterosexist cultural context, the structure of the gang combines with individual shifts in identity to encourage pathways out of straight gangs and pathways into continued involvement with gay gangs.