bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSIV-21 Pellet processing reduces variability of corn soybean meal-based diet digestibility in growing pigs
Authors: Chassé Élisabeth , Guay Frédéric , Létourneau-Montminy Marie-Pierre 
Published: November 2020

Abstract
This study was conducted to determine the effect of pelleting on the digestibility of corn-soybean meal-based diet in growing pigs. Two trials with 6 pigs cannulated at the distal ileum were conducted. In each trial, pigs were assigned to each treatment following a crossover design. In each experiment, the same diet, composed of corn and soybean meal with 10% wheat from two different feed mills, was served in pellet or mash form. Pelleting allowed an increase in digestibility in one of the trials. Apparent ileal digestibility (AID) of dry matter (DM), crude protein (CP) and digestible energy (DE) were improved with pelleting by 8, 12 and 9% (P&lt; 0.01). The AID of amino acids (AA) was also improved (P&lt; 0.05). Apparent total tract digestibility (ATTD) was increased by pelleting in DM, CP and DE by 5, 7 and 6% respectively (P&lt; 0.01). The digestibility of the mash diet in experiment 1 was lower than in the pelleted diet in the experiment 1 and both diets in experiment 2 as shown by the interaction Pelleting X Trial which was significant for the AID and ATTD of DM, CP and DE (P&lt; 0.01). Therefore, in experiment 1, pelleting allows to improve the digestibility of diet to the same level as in experiment 2. The AID of CP was higher by 37% in the mash diet from the second experiment compared to the one in the first experiment. Even though the same ingredients were chosen in the two experiments, this shows the variability in digestibility existing between different feed mills and ingredient sources. This difference was not observed in pelleted diets. The results obtained in these two trials show that pelleting can reduce the variability of digestibility and then give a good digestibility of diets even if the ingredients are of different quality or sources.
PSIX-13 Effects of zinc and copper levels on growth performance of nursery pigs
Authors: Cemin Henrique S , Swalla Luke A , Pietig Jamie L , Hansen Sharlie A , Hansen Ernie L 
Published: November 2020

Abstract
A 20-d experiment was conducted to evaluate the effects of added Zn and Cu on growth performance of nursery pigs. Treatments were arranged in a 2 × 2 factorial treatment structure with main effects of added Zn (100 or 3,000 mg/kg of diet) and Cu (10 or 200 mg/kg of diet). Basal Zn and Cu levels (100 and 10 mg/kg, respectively) were from organic sources, while the added high levels (2,900 and 190 mg/kg, respectively) were from Zn oxide and Cu hydroxychloride. A total of 784 pigs (PIC 337 × Camborough), initially 6.0 kg, were used with 7 replicates per treatment. Experimental diets were corn, soybean meal, and whey permeate-based and contained 1.38% SID Lys. Pigs were weighed and feed disappearance measured to calculate ADG, ADFI, and G:F. Data were analyzed with SAS MIXED procedure. From d 0 to 10, there was no evidence (P &gt; 0.10) for interactive or main effects of Zn or Cu. From d 10 to 20 and overall (d 0 to 20), there was an interaction (P &lt; 0.05) between Zn and Cu for G:F. From d 10 to 20, pigs fed 10 mg/kg added Cu had improved G:F when 3,000 mg/kg added Zn was fed; however, pigs fed 200 mg/kg added Cu had similar G:F regardless of added Zn level. There was no evidence (P &gt; 0.10) for interaction for ADG or ADFI, indicating an additive Zn and Cu effect. Feeding 3,000 mg/kg added Zn or 200 mg/kg added Cu improved ADG, ADFI, and final BW with the highest performance observed when feeding both 3,000 mg/kg added Zn and 200 mg/kg added Cu. In summary, feeding high levels of Zn or Cu did not impact the first 10-d postweaning performance, but Zn and Cu effects were observed in the subsequent 10-d and overall performance.
PSIX-16 Growth performance and blood immune parameters of nursery pigs fed canola meal-based diets
Authors: Hong Jinsu , Scaria Joy , Woyengo Tofuko A 
Published: November 2020

Abstract
Effects of dietary solvent-extracted canola meal (SECM) on growth performance, blood thyroid hormones, and immune parameters of nursery pigs were investigated. A total of 200 weaned pigs (initial body weight = 7.00 kg) were obtained in 2 batches of 100 pigs each. Pigs in each batch were housed in 25 pens (4 pigs/pen) and fed 5 diets in a randomized complete block design. The 5 diets were corn-soybean meal-based diet with 0, 10, 20, 30, or 40% SECM. The diets were fed in 3 phases; Phase 1: d 0 to 7, Phase 2: d 7 to 21, and Phase 3: d 21 to 42. Growth performance was determined by phase. Blood parameters were determined at the end of Phases 1 and 2. Increasing dietary SECM from 0 to 40% resulted in a quadratic increase (P=0.007) in overall (d 0-42) average daily gain (ADG) such that an increase in dietary level of SECM from 0 to 20% resulted in an increase (P&lt; 0.05) in ADG by 14.1%, but an increase in level of SECM from 20 to 40% resulted in a decrease (P&lt; 0.05) in ADG by 11.4 %. Dietary SECM tended to linearly reduce (P=0.05) Phase 2 serum tetraiodothyronine level. An increase in dietary level of SECM from 0 to 20% did not affect the serum IgA level, but an increase in level of dietary level of SECM from 20 to 40% resulted in a decrease (P&lt; 0.05) in the serum IgA level. Serum IgG and TNF-α levels were unaffected by diet. In conclusion, increasing dietary SECM level from 0 to 20% increased growth performance of weaned pigs. However, increasing dietary SECM level from 20 to 40% reduced growth performance and increased immune response. Thus, SECM can be included in diets for nursery pigs at 20% to improve performance.
PSIX-6 Effect of dacitic tuff breccia in nursery diets on pig growth performance, blood parameters, and intestinal morphology
Authors: Garcia Ricardo M , Ferrel Jon , Thayer Morgan T , Mahoney Julie F , Duttlinger Alan W , Chastain Clayton S , Richert Brian T , Radcliffe John S 
Published: November 2020

Abstract
Trace element and mineral intake are important for proper function of the immune system and various metabolic processes. The objective of this experiment was to evaluate the effect of a dacitic tuff breccia (DTB), classified as a hydrated sodium calcium aluminosilicate, in the diet of nursery pigs. Dietary treatments were: 1) Control diet (CON), 2) CON + 0.25% DTB (DTB1), and 3) CON + 0.50% DTB (DTB2). Pigs (6.79 + 0.064 kg; 26d of age) were individually housed and fed dietary treatments for 21 d (n=48; 16/trt). At the end of the experiment, blood, jejunal, and ileal samples were collected for a typical metabolic panel and basic histologic and morphologic measurements. DTB2 fed pigs tended (P=0.07) to have greater ADG than CON and DTB1 fed pigs (392, 397, 430g/d for CON, DTB1, DTB2, respectively), and greater (P=0.008) ADFI (591, 592, 669g/d for CON, DTB1, DTB2, respectively). Gain:Feed was not different (P=0.75) among treatments (0.655, 0.663, 0.644 for CON, DTB1, DTB2, respectively). Crypt depth in the jejunum was greater (P=0.04) for DTB1 compared to CON fed pigs but did not differ from DTB2 fed pigs (254.7, 292.0, 275.6mm for CON, DTB1, DTB2, respectively). Villous height, villous:crypt, number of goblet cells and intraepithelial lymphocytes did not differ among treatments in both ileum and jejunum (P &gt;0.17). Total white blood cell (WBC) count did not differ (P &gt;0.10). However, the proportion of WBC that were monocytes was lower (P=0.024) in DTB1 compared to CON and DTB2 fed pigs (1.27, 0.79, 1.45% for CON, DTB1, DTB2, respectively). Blood Urea Nitrogen (BUN) concentration tended (P=0.06) to be linearly decreased with increasing DTB concentrations (9.27, 8.80, 7.75mg/dL). DTB inclusion at 0.5% in nursery pig diets tended to improve growth performance, while inclusion at 0.25% increased jejunal crypt depth and reduced monocytes:WBC and increasing DTB concentrations tended to decreased BUN linearly.
PSVI-22 Rumen microbiome of beef cattle is modulated by backgrounding systems
Authors: Omontese Bobwealth O , Sharma Ashok K , Langlie Jason , Armstrong Joe , DiCostanzo Alfredo , Webb Megan J , Gomez Andres 
Published: November 2020

Abstract
Backgrounding (BKG) segment in beef production systems is characterized by utilization of different forages which affect growth performance and carcass characteristics. However, it is unclear how BKG systems impact rumen microbiome. We investigated rumen microbiome dynamics of beef calves under different BKG systems. At weaning, Angus and Angus x Simmental beef calves (n = 38) were stratified by age, weight, and sex in a completely randomized design into 1 of 3 BKG treatments for 55 d: 1) perennial pasture (PP; quackgrass, orchardgrass; smooth bromegrass, red clover, and alfalfa); 2) summer annual cover crop (CC; cereal oats, purple top turnips, hunter forage brassica, and graza forage radish); and 3) dry lot (DL; haylage, corn, and DDGS). After BKG, all calves were assigned to a high energy ration in a feedlot. Rumen sample was collected via esophageal tubing at weaning, BKG and feedlot. A total of 190 rumen fluid samples were used to sequence the hypervariable V4 region of the 16S rRNA bacterial gene on an Illumina MiSeq platform. The results showed that BKG systems largely influenced rumen bacterial communities. Specifically, microbiome composition and diversity were not different at weaning, diverged significantly during BKG (Shannon index, Bray Curtis distance metrics; P &lt; 0.001) and homogenized during feedlot. During the BKG segment, the bacterial genera Agrobacterium, Coprococcus, and Ruminococcus were dominant in CC whereas Fibrobacteraceae and Mycoplasmataceae was most dominant in DL. Moreover, rumen microbiome patterns of CC and DL calves showed increased plasticity in early stages of development but not during feedlot with PP showing fewer changes over time. These results indicate that BKG systems significantly modulate the rumen microbiome of beef cattle and, underscore the importance of early developmental stages as potential targets for feeding interventions that can impact the animal microbiome to enhance animal performance.
PSI-11 Moisture Content Throughout the Pelleting Process and Subsequent Effects on Pellet Quality
Authors: Kort Rachel , Wecker Haley , Fiehler Chance , Ogles Aundria , Froetschner Jared , Stark Charles R , Paulk Chad B 
Published: November 2020

Abstract
This experiment was designed to evaluate the effects of steam addition to the conditioner on moisture content throughout the pelleting process and subsequent effects on pellet quality. Treatments consisted of diets pelleted with no steam and steam added to achieve conditioning temperatures of 62.8 and 87.8°C. Conditioner retention time was set at 30 sec and diets were pelleted with a 6.4×63.5 mm pellet die. Pellet samples were collected and immediately placed in an experimental counter-flow cooler for 15 min. All treatments were replicated at 3 separate time points to provide 3 replicates per treatment. Mash (M), conditioned mash (CM), hot pellets (HP), and cooled pellet (CP) samples were collected for moisture content analysis and CP for pellet durability index (PDI). Data were analyzed with pelleting run as the experimental unit and time period as the blocking factor. Moisture samples were analyzed as a 3×4 factorial of steam-conditioning and sample location. There was a steam-conditioning×sample interaction (P&lt; 0.01) for moisture. Mash samples for all treatments were similar (13.3%; 36.2°C). For the no steam treatment, there was no difference in moisture content for the M, CM, and HP; however, moisture decreased in CP, with samples having 13.4, 13.1, 12.9, and 12.0% moisture, respectively. For the 62.7°C treatment, there was an increase in moisture from M to CM, followed by a decrease in both HP and CP, with samples having 13.2, 15.3, 14.9, and 12.7% moisture, respectively. For the 87.8°C treatment, moisture increased from M to CM, and decreased in HP and CP with samples having 13.3, 17.3, 16.3, and 13.4% moisture, respectively. Increasing conditioning temperature from no steam to 87.8°C increased (P&lt; 0.01) PDI from 3.3, 59.1, to 91.1%, respectively. In conclusion, increasing feed temperature from 36.2 to 87.8°C via steam addition increased condition mash moisture content by 4.2% resulting in improved pellet quality.
PSIII-10 The association of genes involved in mitochondrial function with growth, size, and feed efficiency traits in developing beef heifers
Authors: Schmoker Samantha J , Bhowmik Nayan , Dahlen Carl R , Swanson Kendall C , Ringwall Kris A , Hulsman Hanna Lauren L 
Published: November 2020

Abstract
The objective was to determine if genetic markers related to mitochondrial function are associated with growth and feed intake of Bos taurus heifers fed forage-based diets. Weight data collected at birth, weaning, and as yearlings were available on 373 heifers. As yearlings, feed intake was measured using an Insentec system. Primary ancestral breeds of heifers included 204 British, 66 Continental European, 68 American Aberdeen, 29 British mix, and 6 American Aberdeen mix. Traits included DMI, ADG, G:F, adjusted birth weight (BWT), adjusted 205 weaning weight (WW), and average of start and end feed trial weight (FWT) and body volume (VOL). Heifers were genotyped using Neogen GGP150HD for beef cattle. Markers (n=56) were extracted if located within or flanking AMPK, PPARGC1A, FGF2, and SIRT1 or on mitochondrial DNA using ARS-UCD1.2 coordinates. After quality checks (minor allele frequency and call rate), 44 markers remained. No mitochondrial markers passed quality checks. For each trait, each marker was fit independently as a fixed effect in a model including year and group based on frame score (n=4), ancestry (n=5), and dam age (n=4; non-adjusted traits only). Pairwise comparisons were done using Tukey-Kramer method. No markers within FGF2 or SIRT1 had significant associations. Two markers were found significant for PPARGC1A, where Hapmap24121-BTC-039009 (P=0.0344) showed the C allele increased DMI. For weight traits, significant markers surrounding AMPK and within PPARGC1A (n=5; 0.0009≤P≤ 0.0468) identified dominance gene actions that indicate certain variants of the AMPK and PPARGC1A increased weight across time-points. For VOL, 16 of 22 markers within PPARGC1A were significant (0.0068≤P≤0.0467). Of the 16 markers, 14 identified a recessive variant that increased volume, but did not translate to increased weight. Further research is needed to better understand the roles that genetic markers for AMPK and PPARGC1A have on tissue level mitochondrial function and energy metabolism.
25 Determining stability of genomic predictivity in future generations in commercial pig populations
Authors: Hollifield Mary Kate , Lourenco Daniela , Howard Jeremy , Huang Yijian , Misztal Ignacy 
Published: November 2020

Abstract
Genomic predictivity is expected to decay over time as predictions are evaluated to more distant generations. More data increases predictive ability; however, data from distant ancestors may not add a significant amount of value compared to the data from closely related individuals. The objective of this study was to evaluate the decay in genomic predictivity over time and to compare the magnitude of decay when including ancestral data versus data from 2 and 3 most recent generations for body weight at off-test (BW). The data set included 211,812 phenotypic records. The pedigree included 406,983 animals from 2001 to 2020, of which 55,118 were genotyped. A single-trait model was used with all ancestral data and sliding subsets of two- and three-generation intervals. Single-step GBLUP was used to calculate GEBVs. Predictive abilities were calculated by the correlation between GEBVs and adjusted phenotypes. Validation populations consisted of single generations succeeding the training population and continued for all generations available. Predictive ability was slightly higher, with all ancestral data in the training population compared to three- and two-generation intervals. The decay of predictivity was similar when comparing the three training population subsets. The average predictivity for the validation population immediately following the training population was 0.40 for 2016, 0.39 for 2017, 0.35 for 2018, and 0.29 for 2019. Predictive ability reached a maximum in the year 2017 (0.45) for the ancestral training population, 2017 (0.43) for the 3-year training population, and 2016 (0.38) for the 2-year training population. The average decay of predictive ability from the first year after the training population to the second year was -0.08. Realized predictivity is affected by selection pressure. The drop in predictive ability suggests declining heritability. With more data and with consistent selection pressure, predictive abilities should increase.
43 Feed efficiency and behavior are associated with gut microbiome in three breeds of pigs
Authors: Tiezzi Francesco , Bergamaschi Matteo , Howard Jeremy , Maltecca Christian 
Published: November 2020

Abstract
Feed efficiency and behavior are important traits in the pork industry for economic, welfare, and environmental aspects. The gut microbiota plays an important role in nutrient digestibility and it is likely to influence these traits. The aim of this study was to characterize the feed efficiency, feeding behavior and gut microbiome relationships of pigs belonging to three different breeds. Individual body weight, feed intake and rate of Duroc (n = 222), Landrace (n = 244), and Large White (n = 221) pigs were recorded. Rectal fecal samples were collected from each animal at three time points (T1, start of trial; T2, mid-trial; T3, end of trial) and used for microbiome 16S rRNA gene sequencing. Individual feed intake and body weight were edited to obtain average daily gain (ADG), average daily feed intake (ADFI), feed conversion ratio (FCR), average daily feeding rate (ADFR), average feed intake per visit (AFIV), average daily number of visits to feeder (ANVD), average daily occupation time (AOTD), average occupation time per visit (AOTV). The impact of gut microbiome on the traits studied was present and seemed to depend on the breed and the time point of recording. At T1, Oscillibacter and Phascolarctobacterium had negative impact on ANVD and ADFI in Duroc, Ruminococcus had negative impact on ADFI in Landrace and Parvimonas, Escherichia and Anaerovibrio had negative impact on ADFI and ANVD in Large White. At T2, Lactobacillus showed a positive impact on ADFR in Landrace and on ADFI in Large White. At T3, Ruminococcus, Faecalibacterium and Dorea had a positive impact on ADFI in Duroc, Staphylococcous had positive impact on ADFR in Landrace and Peptoniphilus had negative impact on ADFI in Large White. Gut microbiome may have an heterogenous impact on the regulation of feeding behavior and feed efficiency depending on the host genotype.
60 Occurrence of Mycotoxins in 2019 US Corn-based Feed Ingredients
Authors: Schwandt Erin F , Gott Paige N , Hendel Erika G , Ramirez Shelby M , Murugesan G Raj , Hofstetter Ursula 
Published: November 2020

Abstract
Mycotoxins are secondary fungal metabolites that are detrimental to animal health and productivity. This study investigated occurrence and contamination levels of mycotoxins in the 2019 US corn harvest, including corn grain and corn byproduct feed ingredients (distillers dried grains, gluten feed, etc.). Corn samples from the 2019 harvest and corn DDGS and other byproducts from October 2019 through April 2020 were screened via the LC-MS/MS technique for the presence of six major mycotoxin groups: aflatoxins, type A trichothecenes, type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN) and ochratoxin A. Parameters of the main toxins found were compared to the two prior harvest years using the Kruskal-Wallis Test (Prism7, GraphPad, La Jolla, CA) and are presented in Table 1. Mean toxin count per sample in corn grain was similar in 2019 as 2018, with just over two toxins per sample. Mean B-Trich level (parts per billion, ppb) was elevated in 2019 from 2017. Fumonisin contamination increased in 2019 compared to 2017, and prevalence was similar to 2018. Zearalenone contamination and prevalence were similar in 2019 compared to 2018 levels. Co-contamination in corn byproduct feeds was steady, averaging nearly three toxins per sample. Mean B-Trich contamination was higher than the 2017 crop, and FUM contamination was higher in 2019 than 2017 and 2018. Zearalenone levels in 2019 byproducts are the highest seen in the past three years. Preliminary results of the 2019 corn-based feed ingredients survey suggest mycotoxin occurrence and contamination levels are approaching those observed in the challenging 2018 crop. Furthermore, there are continued concerns for co-contamination and greater ZEN contamination that may impose negative effects on growth, health and reproductive performance in ruminants.
63 DNA methylome wide profile associates differentially methylated loci and regions with cow’s ileal lymph node response to Mycobacterium avium subsp. paratuberculosis
Authors: Ibeagha-Awemu Eveline M , Bhattarai Suraj , Dedemaine Pier-Luc , Wang Mengqi , McKay Stephanie D , Zhao Xin , Bissonnette Nathalie 
Published: November 2020

Abstract
Johne’s Disease (JD), caused by Mycobacterium avim spp paratuberculosis (MAP), is a chronic and incurable disease of ruminants with devastating consequences to the dairy industry. MAP can alter the expression of genes and biological processes during the progression of JD. While some studies have examined the role of gene expression regulators like microRNA in the pathogenesis of JD, no study has explored the role of DNA methylation. This study therefore examined the effect of MAP on DNA methylation profile in the ileum lymph node (ILLN) of cows with subclinical MAP infection. DNA from ILLN tissues from five cows positive for MAP (MAP positive) and five negative cows (MAP negative) were extracted and subjected to whole genome bisulfite sequencing and bioinformatics analysis. A total of 6,394 differentially methylated cytosines (DMCs) and 3,946 differentially methylated regions (DMRs) (FDR &lt; 0.05) were identified between MAP positive and negative cows. DMRs were annotated to 2,488 genes, including the promoters of 238 genes. Some genes with hypermethylated promoters like GRB10, EIF4E, SLC30A3, SOX30 or hypomethylated promoters like SLC11A1, HOXA4, SLC18A1 have been associated with JD or mycobacterial infections in cattle and/or humans. Functional annotation of DMR genes indicated enrichment in pathways previously associated with JD or human diseases with similar pathological conditions as JD, such as T/B cell receptor signaling pathway, Th17 cell differentiation, Cell adhesion molecules, Leukocyte transendothelial migration, HIF-1 signaling pathway and Chagas disease. Furthermore, enriched gene ontology terms like negative regulation of immune system process, negative regulation of cytokine secretion/production and negative regulation of inflammatory response suggest that MAP prevented or reduced the host immune response. Our data demonstrate that DNA methylation changes contribute to regulation of host immune responses to MAP infection and may be one of the mechanisms that MAP uses to subvert host immune responses for its long-term survival.
308 Communities of Practices as Preferred Sources of Biosecurity Information in Crisis Situations
Authors: Iverson Joel 
Published: November 2020

Abstract
Communities of practice create opportunities for interorganizational communication within an industry that are immediately adaptable to crisis situations. The collaborative efforts, for example, of extension agents, veterinarians, swine researchers, and industry groups that exist for promoting biosecurity on an ongoing basis can be intensified for responding to or preparing for outbreaks of such diseases as Porcine Epidemic Diarrhea virus or African Swine Fever. This presentation explores the function of communities of practice in such crisis situations. Ultimately, the presentation provides a series of best practices for designing messages that effectively promote biosecurity through strategic communication.