bluuebunny/crossref_metadata_2025_split huggingface dataset:
G protein‐coupled oestrogen receptor promotes cell growth of non‐small cell lung cancer cells via YAP1/QKI/circNOTCH1/m6A methylated NOTCH1 signalling
Authors: Shen Yi , Li Chong , Zhou Lin , Huang Jian‐An 
Published: November 2020

AbstractResults from various studies reveal that the role of G protein‐coupled oestrogen receptor (GPER) is cancer‐context dependent, and the function of GPER in non–small‐cell lung cancer (NSCLC) is still unclear. The present study demonstrated that neoplasm lung tissues expressed higher level of GPER compared with the normal lung tissues. The clinical data also showed that GPER expression level was positively correlated with the tumour stage of NSCLC. Our experimental data confirmed that GPER played an oncogenic role to promote cell growth of NSCLC cells. Mechanistic dissection revealed that GPER could modulate the NOTCH1 pathway to regulate cell growth in NSCLC cells. Further exploration of the mechanism demonstrated that GPER could up‐regulate circNOTCH1, which could compete with NOTCH1 mRNA for METTL14 binding. Because of the lack of m6A modification by METTL14 on the NOTCH1 mRNA, NOTCH1 mRNA was more stable and much easier to undergo protein translation. Subsequently, we found that GPER could prevent YAP1 phosphorylation and promote YAP1‐TEAD's transcriptional regulation on QKI, a transacting RNA‐binding factor involved in circRNA biogenesis, to facilitate circNOTCH1 generation. Supportively, data from preclinical mice model with implantation of H1299 cells also demonstrated that knock‐down of circNOTCH1 could block GPER‐induced NOTCH1 to suppress NSCLC tumour growth. Together, our data showed that GPER could promote NSCLC cell growth via regulating the YAP1/QKI/circNOTCH1/m6A methylated NOTCH1 pathway, and targeting our identified molecules may be a potentially therapeutic approach to suppress NSCLC development.
Long intergenic non‐coding RNA Linc00485 promotes lung cancer progression by modulating miR‐298/c‐Myc axis
Authors: Zhang Zhenyang , Lin Wenwei , Lin Yuhan , Kang Mingqiang , Zhu Jiafu , Tong Zhangwei , Wu Long , Sun Jianhai , Lin Jiangbo 
Published: November 2020

AbstractLong non‐coding RNAs (lncRNAs), which are non‐protein‐coding transcripts, are emerging as novel biomarkers for cancer diagnosis. Their dysregulation is increasingly recognized to contribute to the development and progression of human cancers, including lung cancer. Linc00485 is a newly discovered cancer‐related lncRNA; however, little is known about its role in lung cancer progression. In this study, we found that the expression of Linc00485 was significantly increased in human lung cancer tissue and associated with malignant phenotypes, including tumour‐node‐metastasis (TNM) stage, metastasis and relapse. Furthermore, the proliferative, migratory and invasive abilities of lung cancer cells in vitro were significantly enhanced by overexpression of Linc00485 but inhibited by its silencing. Mechanistically, Linc00485 regulated the expression of c‐Myc by directly binding to miR‐298; the effects of Linc00485 overexpression could be significantly reversed by a c‐Myc inhibitor or small interfering RNA. Xenotransplantation experiments showed that Linc00485 silencing significantly weakened the proliferation potential of A549 cells in vivo. Overall, these findings indicate that Linc00485 overexpression down‐regulates miR‐298, resulting in the up‐regulation of c‐Myc and thereby promoting the development of lung cancer.
Roles of DEMETER in regulating DNA methylation in vegetative tissues and pathogen resistance
Authors: Zeng Wenjie , Huang Huan , Lin Xueqiang , Zhu Chen , Kosami Ken‐ichi , Huang Chaofeng , Zhang Huiming , Duan Cheng‐Guo , Zhu Jian‐Kang , Miki Daisuke 
Published: November 2020

AbstractDNA methylation is an epigenetic mark important for genome stability and gene expression. In Arabidopsis thaliana, the 5‐methylcytosine DNA glycosylase/demethylase DEMETER (DME) controls active DNA demethylation during the reproductive stage; however, the lethality of loss‐of‐function dme mutations has made it difficult to assess DME function in vegetative tissues. Here, we edited DME using clustered regularly interspaced short palindromic repeats (CRISPR) /CRISPR‐associated protein 9 and created three weak dme mutants that produced a few viable seeds. We also performed central cell‐specific complementation in a strong dme mutant and combined this line with mutations in the other three Arabidopsis demethylase genes to generate the dme ros1 dml2 dml3 (drdd) quadruple mutant. A DNA methylome analysis showed that DME is required for DNA demethylation at hundreds of genomic regions in vegetative tissues. A transcriptome analysis of the drdd mutant revealed that DME and the other three demethylases are important for plant responses to biotic and abiotic stresses in vegetative tissues. Despite the limited role of DME in regulating DNA methylation in vegetative tissues, the dme mutants showed increased susceptibility to bacterial and fungal pathogens. Our study highlights the important functions of DME in vegetative tissues and provides valuable genetic tools for future investigations of DNA demethylation in plants.
Necrostatin‐1, RIP1/RIP3 inhibitor, relieves transforming growth factor β‐induced wound‐healing process in formation of hypertrophic scars
Authors: Lin Pei‐Ting , Xue Xiao‐Dong , Zhao Zhong‐Dong , Lu Jun‐Yang , Xie Pei‐Lin 
Published: November 2020

AbstractBackgroundHypertrophic scars (HS) are common pathologic processes emerged during wound‐healing process. The receptor‐interacting protein kinase (RIP) might participate in keloid formation.AimsThis study aimed to investigate Necrostatin‐1 (Nec‐1), a RIP1/RIP3 inhibitor, in the formation of hypertrophic scar.MethodsHuman hypertrophic scar fibroblasts (HSF) were extracted from patients with hypertrophic scar. Transforming growth factor‐β1 (TGF‐β1) was performed to induce wound‐healing process including cell proliferation (CCK‐8, Flow cytometry, and Western blot), migration (Transwell assay, Western blot), collagen production (Western blot), and extracellular matrix dysfunction (Western blotting and immunofluorescence).ResultsOur results reported that Nec‐1 inhibited TGF‐β1‐induced cell proliferation and promoted G0/G1 phase arrest in HSF. In addition, Nec‐1 attenuated TGF‐β1‐induced cell migration and inhibited the expression of MMP2 and MMP9 in TGF‐β1‐induced HSF. Besides, Nec‐1 also reduced TGF‐β1‐induced collagen production and α‐smooth muscle actin expression in HSF.ConclusionsThe present data in this study showed the potential role of Nec‐1 as a novel treatment for HS.
Modeling lipid layers of atopic skin and observation of changes in lipid layer properties with changes in ceramide content
Authors: Jung In‐Keun , Choi Joonho , Nam Jin , No Kyoung Tai 
Published: November 2020

AbstractBackgroundStudies have shown that there is a high correlation between atopic dermatitis and decrease in ceramide content in the lipid bilayer of skin. Moreover, it has been shown that the reduction in ceramide content in the stratum corneum is unique to atopic dermatitis, indicating that there are particular structural differences between the lipid bilayers of normal and atopic skin.AimThis study aimed to compare the lipid bilayer of the atopic skin with that of the healthy skin and to establish a structural model of the lipid bilayer for atopy.MethodsMolecular dynamics simulations were performed using NAMD 2.8. Models of lipid bilayers of normal skin and atopic skin, and a model of lipid bilayer containing only ceramide were built with CHARMM‐GUI. The thickness, area occupied per lipid, and alignment of lipids were compared among the three models. Potential mean force (PMF) of the sodium laureth sulfate (SLES) on lipid bilayers was calculated to predict the affinity between SLES and lipid bilayers.ResultsPotential mean force calculations showed that the lipid bilayer of atopic skin was able to absorb the surfactant more easily than that of normal skin.ConclusionsWhen the ceramide ratio is low, the thickness of lipid bilayer is reduced and its structure is weakened. Other structural differences between the lipid layers of normal and atopic skin included increased area per lipid and poor alignment of lipids. Further, the atopy lipid bilayer model was found to absorb more SLES than the normal skin lipid bilayer model.
The use of biostatic human amnion and platelet‐rich plasma in topical treatment of toxic epidermal necrolysis—A case report
Authors: Klama‐Baryła Agnieszka , Strzelec Przemysław , Pawlik‐Knapik Danuta , Cholewa Zbigniew , Szapski Michał , Kitala Diana , Łabuś Wojciech , Kraut Małgorzata , Smętek Wojciech , Kucharzewski Marek 
Published: November 2020

AbstractBackgroundToxic epidermal necrolysis (TEN) (also known as Lyell syndrome) and Stevens‐Johnson syndrome (SJS) are life‐threatening mucocutaneous blistering diseases. They are characterized by generalized blisters and epidermal inflammation, most likely resulting from the administration or interaction of medicines.AimsTo report potential new method in the treatment of TEN.Patients/MethodsThis article presents a case report of a 35‐year‐old man suffering from TEN covering about 95% of his body surface. Lesions occurred in the patient during antiepileptic therapy, after taking simultaneously amoxicillin (with clavulanic acid) and naproxen followed by lamotrigine treatment. Standard general treatment was performed. Intravenous feeding was necessary. Due to acute respiratory failure, the patient required mechanical ventilation. Two methods were combined in topical treatment: application of platelet‐rich plasma (PRP) and a simultaneous biostatic human amnion transplant.ResultsIn the presented case, the combination of both methods contributed to a significant acceleration of wound healing. After the application of PRP and biostatic amnion transplantation, the healing of wounds on the back and posterior surfaces of the legs was completed after six days. The surgical treatment most probably contributed to a significant acceleration of wound healing.ConclusionThe case report shows that topical TEN/SJS treatment with biostatic human amnion and PRP has a positive clinical effect and may be a new method of treatment of TEN.
Ethics, health disparities, and discourses in oncology nursing’s research: If we know the problems, why are we asking the wrong questions?
Authors: Rabelais Em , Walker Rachel K. 
Published: November 2020

AbstractAims and ObjectivesTo disrupt conflations between ‘health disparities research’ and critical engagement with racism, whiteness, other oppressions and our profession's ongoing roles in white supremacy.BackgroundIn Oncology Nursing Society's (ONS) 2019–2023 research agenda, health disparities are highlighted as a top priority for nursing knowledge generation and intervention. The document concludes needs for increased ‘minority and vulnerable population’ participation in cancer clinical trials, reduced financial toxicity, behavioural interventions for risk reduction, incorporation of social determinants of health and technology to promote rural access to high‐quality care.DesignIn this critical resistive, theoretical and ethical analysis on current discourses on health disparities research in oncology nursing, we ask: (a) What forces (stated and unstated) shape current oncology nursing discourses about health disparities?; (b) What assumptions about health and power are embedded in these discourses?; (c) Are we, as nurses and scientists, asking the right questions?MethodsLine‐by‐line analysis of the ONS Research Agenda for 2019–2023 ‘Health Disparities’ section.ResultsThe health disparities described in this report are not new to the literature, nor are many of the proposed solutions. As noted, disparities such as disproportionate cancer‐related morbidity and mortality across identities (gender, race and sexual orientation) have not improved and some have worsened over several decades.ConclusionsThat discourses on prioritising cancer‐related health disparities persist while disparity‐related outcomes remain largely unchanged presents challenges—both moral and pragmatic. We must ask, ‘Rather than the concept of “health disparities,” as presently understood in cancer nursing, what is the better approach to examine health equity and ethical nursing research practices?’Relevance to Clinical PracticeThis paper offers several starting places for nurses, especially with the following questions: ‘Who does this harm?’ Answer then revise: ‘Who might this harm now?’ Answer then revise: ‘Are these harms worth the activity?’ And repeat this process.
Metal hypersensitivity screening among frontline healthcare workers—A descriptive study
Authors: Dordunoo Dzifa , Hass Michelle , Smith Catherine , Aviles‐Granados Martha L. , Weinzierl Miriam , Anaman‐Torgbor Judith A. , Shaik Ajijoon , Mallidou Anastasia , Adib Farzad 
Published: November 2020

AbstractAims and objectivesThe study aims were to (a) assess allergy screening practices, (b) determine the awareness of metal hypersensitivity among frontline healthcare workers and (c) examine perceived barriers to implementing metal hypersensitivity screening into clinical practice.BackgroundAdverse device‐related events, such as hypersensitivity to metals, are well documented in the literature. Hypersensitivity to metal is a type IV T‐cell‐mediated reaction that can occur after cardiac, orthopaedic, dental, gynaecological and neurosurgical procedures where a device with metal components is implanted into the body. Patients with hypersensitivity to metal are likely to experience delayed healing, implant failure and stent restenosis. Identifying patients with a history of metal hypersensitivity reaction could mitigate the risk of poor outcomes following device implant. Yet in clinical practice, healthcare workers do not routinely ask about the history of metal hypersensitivity when documenting allergies. The existing literature does not report why this is not included in allergy assessment.DesignFollowing the STROBE checklist, a cross‐sectional, descriptive study was conducted.MethodsFrontline healthcare workers were recruited using professional contacts and social online media to complete an online questionnaire. Quantitative data were summarised descriptively while thematic analysis was used to examine barriers to implementation.ResultsThree hundred forty‐five participants from 14 countries completed the questionnaire, with the majority (187/54%) practicing in Canada, in general medicine and intensive care units. Ninety per cent of the participants did not routinely ask about metal hypersensitivity when evaluating allergy history. Of the respondents, 86% were unaware of the association between metal hypersensitivity and poor patient outcomes. After presented with the evidence, 81% indicated they were likely or very likely to incorporate the evidence into their clinical practice. Common themes about barriers to implementing were ‘Standards of Practice’, ‘Knowledge’ and ‘Futility of Screening’.ConclusionThe findings suggest lack of awareness as the main reason for not including metal in routine allergy assessment.
Specific taxation, asymmetric costs, and endogenous quality
Authors: Schröder Philipp J. H. , Sørensen Allan 
Published: November 2020

AbstractThis paper shows how a specific tax—in contrast to an ad valorem tax—alters industry structure and firm‐level performance in a monopolistic competition framework, where firms chose product quality endogenously and differ exogenously in productivity (i.e., marginal production efficiency). Industry equilibrium mechanisms and selection based on productivity play a significant role: A specific tax shifts market shares and profits toward firms with costs and prices above the industry average at the expense of low‐cost firms. This reallocation of market shares releases a novel scale effect such that low‐cost firms may quality downgrade, while high‐cost firms always quality upgrade. There exists a parameter subspace, where this combines to a decrease on average quality for the industry. In comparison: An ad valorem tax only reduces the number of firms/varieties in the industry due to demand absorption, but affects neither firm‐level performance nor industry structure.
Effects of impaired microvascular flow regulation on metabolism‐perfusion matching and organ function
Authors: Roy Tuhin K. , Secomb Timothy W. 
Published: November 2020

AbstractImpaired tissue oxygen delivery is a major cause of organ damage and failure in critically ill patients, which can occur even when systemic parameters, including cardiac output and arterial hemoglobin saturation, are close to normal. This review addresses oxygen transport mechanisms at the microcirculatory scale, and how hypoxia may occur in spite of adequate convective oxygen supply. The structure of the microcirculation is intrinsically heterogeneous, with wide variations in vessel diameters and flow pathway lengths, and consequently also in blood flow rates and oxygen levels. The dynamic processes of structural adaptation and flow regulation continually adjust microvessel diameters to compensate for heterogeneity, redistributing flow according to metabolic needs to ensure adequate tissue oxygenation. A key role in flow regulation is played by conducted responses, which are generated and propagated by endothelial cells and signal upstream arterioles to dilate in response to local hypoxia. Several pathophysiological conditions can impair local flow regulation, causing hypoxia and tissue damage leading to organ failure. Therapeutic measures targeted to systemic parameters may not address or may even worsen tissue oxygenation at the microvascular level. Restoration of tissue oxygenation in critically ill patients may depend on restoration of endothelial cell function, including conducted responses.
Museum material of Rhipicephalus sanguineus sensu Roberts (1965) collected in 1902–1964 from Australia is identical to R. sanguineus sensu lato tropical lineage at the mitochondrial DNA 12S rRNA level
Authors: Chandra S. , Halliday B. , Šlapeta J. 
Published: November 2020

AbstractTicks identified as Rhipicephalus sanguineus (Latreille, 1806) have been recorded in Australia since 1896. It is now recognized that ticks identified as R. sanguineus belong to different lineages. Recently, the so‐called temperate lineage has been redescribed as R. sanguineus sensu stricto with a designated neotype. In Australia, the evidence suggests that only R. sanguineus sensu lato tropical lineage exists. We present a genetic evaluation of R. sanguineus sensu Roberts (1965) from museum material that was evaluated or identified by Roberts. We evaluated 12 museum specimens collected between 1913 and 1964, from various locations around Australia at the molecular level. DNA isolation was successful for 4/12 specimens. All four museum specimens were 100% identical to each other and to the reference R. sanguineus s.l. tropical lineage. Measurements of morphologically significant structures for male and female museum and contemporary tick specimens were obtained. The morphologically significant structures revealed there was no significant difference (ANOVA, P > 0.05) between these groups of male and female ticks. This study updates and supplement the comprehensive descriptions provided by Roberts (1965), which genetically belong to R. sanguineus s.l. tropical lineage.
Summation anaphylaxis: A challenging diagnosis
Authors: Calvani Mauro , Anania Caterina , Cuomo Barbara , D’Auria Enza , Decimo Fabio , Indirli Giovanni Cosimo , Mastrorilli Violetta , Santoro Angelica , Sartorio Marco U. A. , Veronelli Elisabetta 
Published: November 2020

AbstractAnaphylaxis is the most severe of allergic reactions. The most frequent triggers of anaphylaxis in childhood are food, insect venom, drugs, exercise, etc. In some cases, the presence of more than one trigger is necessary for the allergic reaction, while one trigger alone is tolerated. This rare condition is called summation anaphylaxis (SA). Food‐dependent exercise‐induced anaphylaxis is the most well‐known SA. However, SA may also occur with the association between food and/or exercise plus one or more of the following other cofactors, such as drugs, especially non‐steroidal anti‐inflammatory (NSAID), alcohol, infections, temperature variation, and menstrual cycle. SA can explain some cases of idiopathic anaphylaxis, as well as cases of an apparent breakdown in a previously acquired tolerance for food, or finally, when faced with a suggestive clinical history of food allergy or exercise anaphylaxis and the provocation test is negative. In these situations, a more careful clinical history looking for other cofactors is necessary.
SARS‐CoV‐2 infection and neonates: a review of evidence and unresolved questions
Authors: Pietrasanta Carlo , Ronchi Andrea , Schena Federico , Ballerini Claudia , Testa Lea , Artieri Giacomo , Mercadante Domenica , Mosca Fabio , Pugni Lorenza 
Published: November 2020

AbstractSARS‐CoV‐2 infection in the neonatal period poses previously unmet challenges to obstetricians and neonatologists, but several key questions are yet to be answered. Few cases of presumed in utero vertical transmission of the virus from infected mothers to fetuses have been reported, but stronger evidence is needed, from larger datasets with multiple biospecimens rigorously analyzed. Whether acquired before or after birth, SARS‐CoV‐2 infection in neonates can be symptomatic, but our comprehension of neonatal immune response and the subsequent clinical characteristics of COVID‐19 in early life are incomplete. Finally, the pandemic challenged several dogmas regarding the management of mother‐infant dyads, and again more robust data are needed to support the formulation of evidence‐based guidelines. Here, we briefly summarize existing evidence and key unresolved questions about SARS‐CoV‐2 infection and COVID‐19 in the neonatal period.
ControL'Asma Project: new insights
Authors: Tosca Maria Angela , Licari Amelia , Pistorio Angela , Marseglia Gian Luigi , Ciprandi Giorgio ,   “ControL’Asma” Study Group
Published: November 2020

AbstractThe Italian Society of Paediatric Allergy and Immunology has developed the project “ControL’Asma," a nationwide study involved ten third‐level pediatric allergy clinics across Italy. The first findings of the “ControL’Asma” project showed that asthma was more frequent in males, had an early onset and allergic phenotype with very frequent rhinitis comorbidity, uncontrolled, and partly controlled asthma affected about the half of subjects, and the assessment of asthma symptom perception by VAS could be a reliable tool in the asthma management.
Defining the clinical phenotype of COVID‐19 in children
Authors: Giacomet Vania , Stracuzzi Marta , Paradiso Laura , Di Cosimo Maria Elisabetta , Rubinacci Valeria , Zuccotti GianVincenzo 
Published: November 2020

AbstractOn January 7, 2020, a novel coronavirus (SARS‐CoV‐2) was identified as the causative agent of a cluster of pneumonia of unknown origin detected in Wuhan City by Chinese authorities. Since SARS‐CoV‐2 discovery, the corresponding disease (COVID‐19) has rapidly expanded throughout the globe, making as a consequence the World Health Organization (WHO) declaring a pandemic. As of May 19, 2020, over 4.806.299 cases of COVID‐19 had been confirmed worldwide, with more than 318.599 deaths.