bluuebunny/crossref_metadata_2025_split huggingface dataset:
89 Controlling intrinsic pathways to optimize efficiencies of mineral and vitamin use by sows
Authors: Crenshaw Thomas D , Capdeville Mariola Grez , Amundson Laura A 
Published: November 2020

Abstract
Nutrient efficiency concepts seem to have been ignored in applications of diet formulations for the breeding herd, especially for minerals and vitamins. Historically, gestating sows were notably the animal that could withstand marginal, or minimal fortifications of nutrients. The sow was resilient to nutrient inputs. Ample examples illustrate the ability of sows to adapt to marginal nutrient inputs by improvements in efficiency, and thus, avoid reduced productivity. Over-supplementation of nutrients precludes adaptive improvements in efficiency and leads to substantial environmental impacts and economic losses. Failure to focus on optimizing nutrient efficiency contributes to negative consumer perceptions, which imply that modern swine production is not sustainable. Understanding intrinsic pathways provide opportunities to improve nutrient efficiencies for both macro- and micro- minerals, and fat-soluble vitamins. As an example, over-supplementation of 0.10% excess P to sow herd diets equates to an excess of 4,745 metric tons of P that must be accommodated in manure nutrient management plans. The recent global shortage of vitamins captured the attention of nutritionists and raised questions around sufficient safety margins needed for vitamins. Based on our research efforts, the minimal vitamin D requirement for sows is likely around 300 IU D3/kg diet, yet commercial diets are commonly supplemented at 6X that level. The historical recommendations that 1.5X requirements for fat soluble vitamins and 3X requirements for water soluble vitamins provide sufficient safety margins have been largely ignored in current production applications. Does scientific evidence support such extreme over-supplementation? In conclusion, understanding intrinsic pathways to improve gestating sow nutrient efficiencies provides opportunities for significant impacts on the balance between meeting animal needs and minimizing environmental impacts. As an industry our research efforts and practical applications must focus on positive ways to capture nutrient efficiency, especially in mineral and vitamin fortifications, in order to promote a sustainable industry.
87 Energy and lysine utilization and requirements of lactating sows
Authors: Theil Peter K 
Published: November 2020

Abstract
Energy and lysine are the two most important aspects for sow productivity, but how should we understand such a simple thing as feed efficiency in lactating sows? Feed intake, feed composition, utilization of feed ingredients, milk yield, milk composition and body mobilization are the traits that needs to be integrated to understand how efficiently lactating sows utilize their feed.
173 Lysed Corynebacterium glutamicum cell mass from lysine production as a novel feed additive to enhance gut health and growth of newly-weaned pigs
Authors: Cheng Yi-Chi , Duarte Marcos E , Kim Sung Woo 
Published: November 2020

Abstract
The objective was to determine the functional and nutritional values of Corynebacterium glutamicum Cell Mass (CGCM) on growth performance and gut health of newly-weaned pigs. Forty newly-weaned pigs (21 d of age; initial BW 7.1 ± 0.4 kg) were allotted to 5 dietary treatments based on randomized complete block design with sex and BW as blocks. The lysine broth of CGCM (CJ Bio, Fort Dodge, IA) was homogenized by using French press and dried to obtain lysed CGCM. Dietary treatments were: basal diet with lysed CGCM at 0, 0.7, 1.4, 2.1%, and with 1.4% intact CGCM. Experimental diets were formulated based on nutrient requirements (NRC, 2012) and pigs were fed based on 2 phases (10 and 11 d for each phase). Titanium dioxide (0.4%) was added to phase 2 diets as an indigestible external marker to calculate nutrient digestibility. Feed intake and BW were measured at d 0, 10, and 21. Pigs were euthanized on d 21 to collect proximal and distal jejunal mucosa to measure TNF-α, IL-8, MDA, IgA, and IgG concentrations. Diets and ileal digesta were collected to measure AID. Data were analyzed by SAS using MIXED, REG, and GLM procedures. Overall, increasing daily lysed CGCM intake increased (P &lt; 0.05) ADG (211 to 296 g) and ADFI (432 to 501 g). Increasing levels of lysed CGCM decreased (P &lt; 0.05) MDA and changed (quadratic, P &lt; 0.05) IgA (max: 4.90 ng/mg at 1.13%) and IgG (max: 3.37 ng/mg at 1.04%) in the proximal jejunal mucosa. Increasing daily lysed CGCM intake had quadratic effect (P&lt; 0.05) of protein carbonyl (max: 6.3 μmol/mg at 4.9 g/d). Lysed CGCM potentially benefits growth performance and gut health of newly-weaned pigs by reducing oxidative stress and increasing immune response.
174 The effect of a microbial Muramidase on peptidoglycan content in the gut of swine using in-vitro and in-vivo measures
Authors: McCormack Ursula M , Klausen Mikkel , Laprade Lisa A , Christian Sonja , Frederiksen Carsten Østergaard , Walsh Maria C , Tsai Tsungcheng , Maxwell Charles V , Bradley Casey L 
Published: November 2020

Abstract
Bacterial debris in the gastrointestinal tract (GIT) are continuously being produced by the microbiota present upon bacterial division and death. One of the most abundant components in bacterial debris are peptidoglycans (PGN), a structural cell wall component in gram- positive and negative bacteria. The objective of this work was to investigate if addition of a novel microbial muramidase (Muramidase 007; MUR) that hydrolyzes PGN, would reduce PGN adhesion to porcine intestinal cells in-vitro and hydrolyze PGN in the GIT of swine. Adhesion efficacy of intact and MUR hydrolyzed fluorescein-isothiocyanate (FITC)-labelled PGN were compared using fluorescence-microscopy (3 wells/condition). Catalytic performance of MUR on intact FITC-labelled PGN adhered to intestinal cells were also tested. In-vivo MUR-supplementation at 50,000 LSU/kg diet to gestating and lactating sows and/or their subsequent offspring for 42-d post-weaning was investigated. Mass-spectroscopy was used to quantify soluble and total muramic acid, which is only found in PGN, in the ileal and cecal digesta (8 piglets/treatment) to calculate percentage soluble-PGN. Cell-culture data were analyzed using GraphPad-Prism 8.0 and in-vivo data using mixed-models in JMP 14.0. MUR hydrolyzed PGN adhered 10x less to the IPEC-J2 cell line culture compared to intact-PGN (P&lt; 0.05). In addition, data show that MUR hydrolyzed PGN attached to cell surfaces by 2x, as attached PGN were also reduced by 50% following MUR incubation (P&lt; 0.05). Offering sows MUR-diets had no carryover effect on the percentage soluble PGN in their piglets’ digesta, and there were no interactions observed for sow x piglet x tract neither (P &gt;0.05). Percentage soluble-PGN increased in piglets fed MUR compared to control-piglets (47.18 vs. 29.84% SEM:1.624; P&lt; 0.001) and was higher in cecal digesta compared to ileal digesta (49.91 vs. 27.11%; SEM:1.634; P&lt; 0.001), irrespective of MUR-supplementation. These results suggest that MUR may reduce PGN adhesion to intestinal cells and may hydrolyze PGN found in the GIT.
183 Isoquinoline alkaloids increase apparent ileal digestibility of amino acids and crude protein and apparent total tract digestibility of crude protein by finishing pigs fed corn-soybean meal diets
Authors: Rundle Carly M , Stein Hans H 
Published: November 2020

Abstract
An experiment was conducted to determine if the inclusion of a preparation of isoquinoline alkaloids (IQ) in corn-soybean meal diets fed to finishing pigs increases the apparent ileal digestibility (AID) of amino acids (AA) and crude protein (CP) and the apparent total tract digestibility (ATTD) of CP and gross energy. Twelve ileal cannulated barrows (BW: 77.2 ± 6.23 kg) were allotted to a triplicated 4 x 3 incomplete Latin square design with 4 diets and 9 replicates per diet. Diets were supplemented with either 0, 40, 80, or 160 mg/kg IQ and with 0.40% titanium dioxide as an indigestible marker. Each collection period lasted 14 d with d 1 through 10 as an adaptation to the diets, fecal samples were taken using the grab sampling technique on d 11 and 12, and ileal digesta were collected for 8 h on d 13 and 14. Data were analyzed using the Proc MIXED procedure of SAS. Contrast statements were used to determine linear and quadratic effects of including IQ in the diets. Results were considered significant at P ≤ 0.05 and considered a trend at 0.05 &lt; P ≤ 0.10. Inclusion of IQ resulted in a quadratic increase (P &lt; 0.05) in the AID of CP and AA in corn-soybean meal diets fed to finishing pigs, with the greatest digestibility values generally observed in the 40 mg/kg IQ treatment. Additionally, IQ resulted in a quadratic increase (P = 0.009) in the ATTD of CP, with the greatest digestibility observed in the 80 mg/kg IQ treatment. Therefore, it was concluded that supplementing corn-soybean meal diets with 40 or 80 mg/kg IQ may increase the AID of CP and AA and ATTD of CP by finishing pigs.
185 Effect of dacitic tuff breccia in nursery diets on pig growth performance and nutrient digestibility
Authors: Garcia Ricardo M , Ferrel Jon , Rulon Karissa N , Thayer Morgan T , Mahoney Julie F , Duttlinger Alan W , Whitmore Flannery J , Chastain Clayton S , Radcliffe John S , Richert Brian T 
Published: November 2020

Abstract
For proper function of many metabolic processes trace element and mineral intake is important. The objective of this experiment was to evaluate the addition of a dacitic tuff breccia (DTB), a hydrated sodium calcium aluminosilicate, on nursery pig growth and nutrient digestibility. Dietary treatments were: 1) Control (CON) and 2) CON+0.50% DTB (DTB). Pigs (7.73±0.205 kg; 26 d of age) were individually housed and fed for 20d (n=24, 12/trt). Nutrient digestibility was evaluated using individual metabolism pens for 3d total collection of feces and urine during the last week of evaluation. DTB had no effect on pig performance during week 1 (P &gt;0.28). Week 2 ADG (128 versus 302 g/d; P=0.02), BW (10.63 versus 11.77 kg; P=0.03), and G:F (0.275 versus 0.608; P=0.02) were improved for DTB compared to CON fed pigs. Week 3 ADFI was greater for DTB fed pigs compared to CON (642 vs 768 g/d; P=0.01). Overall (d0-20), DTB fed pigs had greater ADG (279 versus 352 g/d; P=0.04), final BW (13.3 versus 14.8 kg; P=0.04), and numerically greater ADFI (497 versus 558 g/d; P=0.16) and G:F (0.562 versus 0.624; P=0.22) compared to CON. During the collection period, ADG was numerically increased (396 versus 427 g/d; P=0.69) and ADFI (544 vs 668 g/d; P=0.03) was greater for DTB versus CON fed pigs. Nutrient digestibility was not affected by inclusion of DTB in the diet (P &gt;0.05). Digestibility coefficients for CON and DTB, respectively, were: 0.863 and 0.856 for DM; 0.842 and 0.842 for digestible energy; 0.824 and 0.814 for nitrogen; 0.701 and 0.667 for phosphorus; 0.873 and 0.816 for sodium; 0.481 and 0.431 for zinc; 0.517 and 0.458 for iron; and 0.438 and 0.392 for manganese. DTB in nursery pig diets improved growth performance and tended to improve feed intake, but had no effect on nutrient digestibility.
264 Effects of Water Quality on Nursery Pig Performance and Health
Authors: Lozinski Brigit , Frederick Brent , Hilbrands Adrienne , Li Yuzhi , Saqui-Salces Milena , Shurson Gerald C , Urriola Pedro E , Wilson Melissa , Johnston Lee 
Published: November 2020

Abstract
Newly-weaned pigs (n = 450; age = 20 d) were used in a 40-d experiment to determine the effects of water quality on pig performance and health. Pigs were sourced from a single commercial sow farm that was negative for both Porcine Reproductive and Respiratory Syndrome virus and Mycoplasma Pneumonia. Pigs were allotted randomly to 45 pens (10 pigs/pen) and pens were assigned to 1 of 3 water treatments that provided water of differing quality. Waters were selected to represent a wide range of perceived water quality. Water quality was determined based on concentration of analytes including sulfates (1,120; 617; 2 ppm), iron (5.4; 5.2; 1.3 ppm), Total Dissolved Solids (TDS; 1,500; 1,050; 348 ppm), hardness (1,410; 909; 235 mg Eq CaCO3/L), magnesium (171; 91; 21 ppm) and sodium (64; 37; 29 ppm) for waters A, B, and C, respectively. Pigs were housed in an environmentally controlled, power ventilated, confinement nursery barn and were allowed ad libitum access to a common three-phase diet and water across all water treatments. Weekly ADG, ADFI, and G:F were measured and subjective scour score (1=solid feces to 4=liquid feces) was recorded daily through day 7. Data for pig growth performance were analyzed using PROC GLIMMIX with water quality as a fixed effect and pen served as the experimental unit. There were no differences among treatments in ADG, ADFI, and G:F (Table 1). Number of pigs treated with antibiotics throughout the experiment did not differ among treatments as determined by Chi-Square analysis. Average subjective scour score on day 7 of the experiment also did not differ among treatments. In conclusion, performance and health of nursery pigs as measured in this experiment were not influenced by the differing water qualities studied.
269 Effects of high phytase supplementation in lactation diets on sow and litter performance
Authors: Batson Kelsey L , Calderon Cartagena Hilda I , Goodband Robert D , Woodworth Jason C , Tokach Mike D , Dritz Steve S , DeRouchey Joel M 
Published: November 2020

Abstract
A total of 109 sows (Line 241; DNA, Columbus, NE) were used in a study to evaluate the effect of increasing phytase concentration in lactation diets on sow and litter performance. On d 107 of gestation, sows were blocked by body weight and parity and allotted to 1 of 3 dietary treatments of increasing phytase concentration (0, 1,000, or 3,000 FYT/kg; Ronozyme HiPhos 2700; DSM Nutritional Products, Inc., Parsippany, NJ). The control diet contained no phytase and was formulated to contain 0.50% standardized total tract digestible phosphorus (STTD P; 0.45% available P) and 0.62% STTD calcium (0.90% total Ca). The same STTD P and Ca concentrations were formulated for the phytase diets considering a release of 0.132 STTD P and 0.094 STTD Ca in both diets. Diets were fed from d 107 of gestation until weaning (d 18 ± 2). Litters were cross-fostered within treatment until 48 h post-farrowing to equalize litter size. Linear and quadratic response to phytase concentration was evaluated using the lmer function in R. There was no evidence for difference in sow body weight change, farrowing performance, wean-to-estrus interval, or litter size among dietary treatments. Sow average daily feed intake from farrowing to weaning tended to increase (linear, P=0.093) as phytase increased. Although not significant (linear, P =0.226), farrowing duration decreased as phytase increased. Litter weaning weight increased (quadratic, P=0.039) and overall litter gain increased (quadratic, P=0.047) with 1,000 FYT of phytase. In summary, sow feed intake tended to increase linearly with increasing phytase; however, feeding 1,000 FYT/kg maximized overall litter gain and weaning weight. This small-scale study suggests sow and litter performance benefits due to high inclusions of dietary phytase; however, a commercial trial with more sows is warranted to confirm these results.
26 Factors effecting collection characteristics in beef bulls at an artificial insemination facility
Authors: Hartman Ashley , Butler Madison L , McCabe Esther D , Goodenow Nicole , Tucker Sharon , Bormann Jennifer M , Grieger David M 
Published: November 2020

Abstract
Increased genomic information available for young bulls has decreased age at time of semen collection. Factors effecting collection characteristics include collection method (electro ejaculate (EE), or artificial vagina (AV), and the number of ejaculates collected. The objective of this study was to determine the effect of managerial factors on collection characteristics. From 2008 to 2018, 11,642 individual ejaculates were analyzed by a single technician at the Kansas Artificial Breeding Service Unit. Bulls that were not receptive to the AV after 3 or 4 attempts, were subject to EE. Collection characteristics were evaluated using multiple regression models; fixed effects included collection method, sequence of ejaculates collected per day, and were evaluated for their impact on collection characteristics. Progressive motility before freezing was greater (P&lt; 0.0001) for bulls collected with EE compared to AV. Ejaculate volume for EE collections was greater (P&lt; 0.0001) than those collected with AV. Percent spermatozoa with secondary abnormalities was greater (P&lt; 0.05) for bulls collected with EE compared to AV. Concentration of spermatozoa per mL was less (P&lt; 0.0001) for bulls collected with an EE (514 x106) compared to AV (617 x106). Total number of straws frozen per ejaculate were less (P&lt; 0.001) for bulls collected with EE (94) compared to AV (108). The number of ejaculates collected per day was significant for the percent of spermatozoa with secondary abnormalities (P&lt; 0.001). As ejaculate number per day increased, the concentration of spermatozoa decreased (713, 580, 535, and 434 x 106 per ml respectively; P&lt; 0.0001) and the number of straws frozen per ejaculate decreased (123, 107, 93, and 82 respectively; P &lt; 0.0001). In conclusion, AV collections resulted in a higher number of straws frozen. The method of collection could cause a significant impact when collecting young high demand bulls.
29 Influence of dietary manganese source on the corpus luteum proteome in pigs
Authors: Studer Jamie M , Kiefer Zoe , Keating Aileen F , Baumgard Lance H , Olsen Kristin M , Rambo Zachary , Wilson Mark E , Rapp Christof , Ross Jason W 
Published: November 2020

Abstract
Functional corpora lutea (CL) are required for pregnancy establishment and gestational maintenance in swine. Manganese (Mn) could be critical in regulating CL function since it is a cofactor for the enzyme Mn superoxide dismutase (Mn-SOD) as well as enzymes involved in progesterone (P4) synthesis. We hypothesized a more bioavailable dietary Mn source would increase CL Mn content thereby influencing luteal function during the mid-luteal phase of the estrous cycle. Post-pubertal gilts (n = 32) were assigned to one of four gestation diets. The control diet (CON) met or exceeded NRC requirements and was formulated to contain 20 ppm added Mn in the form of Mn sulfate. Three additional diets included 20 (TRT1), 40 (TRT2) or 60 (TRT3) ppm Mn from a Mn-amino acid complex (Availa-Mn; Zinpro Corporation) in place of Mn sulfate. Dietary treatment began at estrus synchronization onset and continued through D12 of the ensuing estrous cycle when gilts were euthanized. Mn content increased (P ≤ 0.06) 19, 21 and 24% in CLs of gilts fed TRT1, TRT2, and TRT3, respectively, and luteal P4 concentration decreased (P ≤ 0.03) 25, 26, and 32% in gilts fed TRT1, TRT2, and TRT3, respectively, compared to CON. Total CL protein was extracted and liquid chromatography with tandem mass spectrometry (LC-MS/MS) was performed to assess global protein abundance. Compared to CON, 29, 105, and 118 proteins were differentially abundant (P &lt; 0.01) in TRT1, TRT2, and TRT3, respectively. KEGG pathway analysis revealed proteins involved in P4 signaling (membrane-associated P4 receptor component 2) and cholesterol synthesis and transport (mevalonate kinase, diphosphomevalonate decarboxylase, low density lipoprotein receptor) were downregulated in response to Availa-Mn. Collectively, these data support the posit that dietary Mn source affects Mn accumulation and P4 concentration in CL tissue and influences protein abundance which may affect CL function.
231 Impact of Brachyspira hyodysentariae on intestinal function and integrity
Authors: Helm Emma T , Lin Susanne J , Gabler Nicholas , Burrough Eric R 
Published: November 2020

Abstract
Swine dysentery (SD) induced by Brachyspira hyodysentariae (Bhyo) causes colitis and mucohemorrhagic diarrhea in grow-finish pigs, however little is known about the physiological changes that occur to the gastrointestinal tract during Bhyo infection. Thus, the objective of this study was to evaluate the impact of a Bhyo challenge on intestinal function and integrity of pigs fed two divergent diets. A total of 36 Bhyo negative gilts (24.3 ± 3.6 kg BW) were selected and assigned to one of three treatment groups (n=12 pigs/trt): 1) Bhyo negative, 20% DDGS diet (CON), 2) Bhyo challenged, 20% DDGS diet (DDGS), and 3) Bhyo challenged, 10% DDGS, 5% beet pulp and 5% resistant potato starch diet (RS). Pigs were fed diets 21 days prior to challenge and on days post inoculation (dpi) 0 and 1, pigs were inoculated with Bhyo or sham. Fecal samples were collected for ATTD and pigs were euthanized for colon collection within 72 hours of initial observation of clinical SD, or at the end of the study (dpi 10-16). Tissues were assessed for ex vivo measures of intestinal integrity and mitochondrial function. The challenge resulted in high morbidity, with 88% of DDGS and RS pigs developing clinical SD. Colon transepithelial resistance was increased in DDGS pigs compared with CON and RS pigs (P=0.005), and colon macromolecule permeability was reduced in both DDGS and RS pigs compared with CON pigs (P=0.006), likely due to mucoid discharge. Colonic mitochondrial oxygen consumption was not impacted by treatment (P &gt;0.10). Further, ATTD of DM, OM, N, and GE were reduced in DDGS pigs compared with CON pigs (P&lt; 0.001), whilst nutrient digestibility was not reduced in RS pigs. Taken together, these data show Bhyo does not appear to reduce ex vivo colonic integrity. Further, the RS diet may reduce severity of a Bhyo challenge.
232 Reduced growth performance during viral challenge primarily results from disease hypophagia
Authors: Todd Blaire , Becker Larissa , Gabler Nicholas 
Published: November 2020

Abstract
Reductions in voluntary feed intake during disease, or disease hypophagia, is a common phenotype observed in nearly all species. However, the extent to which disease hypophagia explains attenuated pig performance during disease is poorly defined. The objective of this study was to describe the extent to which hypophagia explains pig performance during a Porcine Reproductive and Respiratory Syndrome (PRRS) virus challenge. Twenty-four PRRS naïve barrows (12.8 ± 0.73 kg BW; Landrace x Large White, PIC) were selected, blocked by body weight, and allotted across three treatments (n=8/trt): 1) PRRS-naïve, ad libitum fed (Ad), 2) PRRS-inoculated, ad libitum fed (PRRS), and 3) PRRS-naïve, pair-fed daily to the PRRS pigs (PF) to mimic disease feed intake. All pigs were individually penned and after a 4-day acclimation, on days post inoculation (dpi) 0, PRRS pigs were inoculated with PRRS virus. Over 7 dpi, daily feed intake and body temperatures (BT), and 7 day ADG, ADFI, and G:F were assessed. Over the 7-day challenge period, a treatment x time interaction (P &lt; 0.0001) was reported for ADFI in which PRRS and PF pigs’ ADFI was reduced by 51% compared with Ad pigs. Compared with Ad pigs, PRRS and PF pigs end BW was reduced by 23% (P = 0.004). The Ad pigs had greater ADG compared with PF and PRRS pigs (0.64, 0.12, -0.04 g/d, respectively, P &lt; 0.0001). Overall G:F was reduced by 112% and 53% in the PRRS and PF pigs, respectively, compared with Ad pigs (P &lt; 0.0001). A treatment x dpi effect was reported in core BT, in which PF pigs; BTs reduced over time compared with Ad and PRRS pigs (38.7, 39.4, 39.6 °C, respectively, P = 0.001). In summary, disease hypophagia explains much of the reduced growth observed during PRRS challenge.