bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of 24 protocols for the production of platelet-rich fibrin
Authors: Miron Richard J , Chai Jihua , Fujioka-Kobayashi Masako , Sculean Anton , Zhang Yufeng 
Published: April 2020

Abstract
Background

The aim of this study was to evaluate 24 protocols for the production of platelet rich fibrin (PRF) produced via horizontal centrifugation to better understand cell separation following protocols at various times and speeds.

Methods

All protocols were compared utilizing a recent method to quantify cells in PRF in 1 mL sequential layers pipetted from the upper layer downwards until all 10 mL were harvested. In total, 960 complete blood counts (CBCs) were investigated. Both solid and liquid-based PRF protocols were investigated following 24 protocols involving 6 relative centrifugal force (RCF) values (100,200,400,700,1000 and 1200g) at 4 centrifugation times (3,5,8 and 12 minutes).

Results

In general, platelets could more easily accumulate in the upper 4 layers when compared to leukocytes owing to their lower cellular density. Protocol time seemed to have a greater impact on the final cell layer separation when compared to the effect of speed. Protocols of greater than 8 minutes at 400 g led to no leukocyte accumulation in the upper PRF layers (found specifically within the buffy coat). Protocols at or below 200 g were unable to effectively accumulate platelets/leukocytes. The optimal centrifugation speed and time for solid-PRF ranged between 400 -700 g for 8 minutes. It was noted that variability in patient baseline platelet/leukocyte/erythrocyte counts (hematocrit) significantly affected cell layer separation. This finding was more pronounced at lower centrifugation speeds.

Conclusions

Within the investigated ranges, a protocol of 700 g for 8 minutes presented the highest yield of platelets/leukocytes evenly distributed throughout the upper PRF layers.
Qualitative Study of Socio-Cultural Challenges in the Nursing Profession in Pakistan
Authors: Abbas Sidra , Zakar Rubeena , Fischer Florian 
Published: April 2020

Abstract
Background: In a patriarchal social system, a women-dominated profession like nursing is mostly seen as a disempowered group due to its stereotypical image and negative connotations. The low social prestige of this profession is based on the roles typically assigned to men and women to maintain gender identity according to their performance and embodiment. The aim of this study was to explore the social and cultural challenges faced by nurses while creating their professional image within the regional context of Lahore (Punjab) in Pakistan.

Methods: A qualitative research design was chosen to conduct one-to-one, in-depth interviews with twelve nurses. Recruitment was based on purposive sampling from three large public hospitals in Lahore to learn about nurses’ perceptions of social and cultural challenges in the nursing profession. A thematic analysis was conducted using the data analysis software package NVIVO 12 Plus.

Results: Cultural values give preference for female nurses. We have identified four major themes related to the social and cultural challenges facing the nursing profession: 1) gender-segregated profession, 2) inappropriate portrayals by the media, 3) issues around marriage settlement, and 4) identity from a religious perspective. These conflicts are affecting the professional status and changing perceptions of nurses, who either do not choose to remain in the nursing profession or do not recommend nursing as a career option. These ongoing constraints are still perpetuating and increasing shortage of nurses within the Pakistani healthcare system.

Conclusion: The present study solely highlights nurses’ perspectives on redefining gender roles and gender integration within the nursing profession. It argues that there is a need for positive portrayals in the media for the removal of public misperceptions related to nursing. This would reduce the shortage of nurses along with increasing retention and improving the quality of healthcare delivered to the public.
Gastric Artery Embolization: Studying Effects of Catheter Type and Injection Method on Bead Distributions within a Benchtop Arterial Model
Authors: Jernigan Shaphan R , Osborne Jason A , Buckner Gregory D 
Published: April 2020

Abstract
Aims: To investigate the effect of catheter type and injection method on microsphere distributions, specifically vessel targeting accuracy.
Materials and Methods: The study utilized three catheter types (a standard micro-catheter, a Surefire anti-reflux catheter, and an Endobar occlusion balloon catheter) and both manual and computer-controlled injection schemes. A closed-loop, dynamically pressurized surrogate arterial system was assembled to replicate arterial flow for bariatric embolization procedures. Four vessel branches immediately distal to the injection site were targeted for embolization. Embolic microspheres were injected into the model using three catheter types (a standard micro-catheter, a Surefire anti-reflux catheter, and an Endobar occlusion balloon catheter) and both manual and computer-controlled injections.
Results: Across all injection methods, the catheter effect on the proportion of microspheres to target vessels (vs. non-target vessels) was significant (p=0.005). The catheter effect on the number of non-target vessels embolized was significant (p=0.059). Across all catheter types, the injection method effect was not statistically significant for either of two outcome measures (percent microspheres to target vessels: p=0.265, number of non-target vessels embolized: p=0.148).
Conclusion: Catheter type had a significant effect on targeting accuracy across all injection methods. The Endobar catheter exhibited a higher targeting accuracy in pairwise comparisons with the other two injection catheters across all injection schemes and when considering the Endobar catheter with the manifold injection method vs. each of the catheters with the manual injection method; the differences were significant in 3 of 4 analyses. The injection method effect was not statistically significant across all catheter types and when considering the Endobar catheter/Endobar manifold combination vs. Endobar catheter injections with manual and pressure-replicated methods.
Is there a relationship between moral competencies and the formation of professional identity among nursing students?
Authors: Haghighat sahar , borhani fariba , ranjbar hadi 
Published: April 2020

Abstract
Background: Moral care provision is an essential part of nursing work. Nursing schools are responsible for developing moral competencies in nursing students. The aim of this study was to investigate the relationship between moral competencies and the formation of professional identity among nursing students .
Methods: This study was designed as a descriptive-correlational study. The study population consisted of nursing students who were enrolled in nursing schools at the time of the study. Two hundred and twenty-one nursing students completed the study tools. The research tools were a demographic questionnaire, Moral Development Scale for Professionals (MDSP), and Professional Identity Scale for Nursing Students (PISNS). Results: The mean (SD) of MDSP and PISNS scores were 45.69±5.90 and 55.61±12.75, respectively. There was a significant statistical relationship between MSDP and PISNS scores (p<0.05). A significant equation was found (f (2, 218) = 16.68, p<0.001) with an R2 of 0.113. The MSDP scores increased 0.136 for each score of PISNS, and married students had 2.452 scores higher than single students.
Conclusions: There was a significant correlation between the formation of professional identity and development of morality in nursing students. By supporting the formation of professional identity, nursing schools can help the development of moral competencies in nursing students.
A non-destructive method to quantify leaf starch content in red clover
Authors: Frey Lea Antonia , Baumann Philipp , Aasen Helge , Studer Bruno , Kölliker Roland 
Published: April 2020

Abstract
Background: Grassland-based ruminant livestock production provides a sustainable alternative to intensive production systems relying on concentrated feeds. However, grassland-based roughage often lacks the energy content required to meet the productivity potential of modern livestock breeds. Forage legumes, such as red clover, with increased starch content could partly replace maize and cereal supplements. However, breeding for increased starch content requires efficient phenotyping methods. This study is unique in evaluating a non-destructive hyperspectral imaging approach to estimate leaf starch content in red clover for enabling efficient development of high starch red clover genotypes.

Results: We assessed prediction performance of partial least square regression models (PLSR) using cross-validation, and validated model performance with an independent test set. Starch content of the training set ranged from 0.1 to 120.3 mg g-1 DW. The best cross-validated PLSR model explained 56% of the measured variation and yielded a root mean square error (RMSE) of 17 mg g-1 DW. Model performance decreased when applying the trained model on the independent test set (RMSE = 29 mg g-1 DW, R2 = 0.36). Different variable selection methods did not increase model performance.

Conclusion: The non-destructive spectral method presented here, provides a tool to detect large differences in leaf starch content of red clover. The major benefit of the method is that it can be repeatedly applied to the same plants, thus providing a means to follow starch concentrations over time and over a broad range of environments.
Percutaneous stent placement for malignant hilar biliary obstruction: side-by-side versus stent-in-stent technique
Authors: Zhou Wei-zhong , Liu Sheng , Yang Zheng-Qiang , Xian Yu-Tao , Xu Hong-Dou , Wu Jun-Zheng , Shi Hai-bin 
Published: April 2020

Abstract
Background: Currently, side-by-side (SBS) and stent-in-stent (SIS) are the two main techniques for stent deployment to treat hilar biliary obstructions. Previous studies comparing these two techniques are very limited, and thus, no consensus has been reached on which technique is better. The purpose of this study is to compare the clinical efficacy and safety of SBS and SIS deployment via a percutaneous approach for malignant hilar biliary obstruction.Methods: From July 2012 to April 2019, 65 patients with malignant hilar biliary obstruction who underwent bilateral stenting using either the SBS or SIS techniques were included in this study. Among them, 27 patients underwent SIS stent insertion (SIS group), and the remaining 38 patients underwent SBS stent insertion (SBS group). Technical success, improvement of jaundice, complications, duration of stent patency, and overall survival were evaluated.Results: Technical success was achieved in all patients in the two groups. The serum bilirubin level decreased more rapidly 1 week after the procedures in the SBS group than in the SIS group (P=0.02). Although the total complication rate did not differ between the two groups, cholangitis was found to be more frequent in the SIS group (P=0.04). The median stent patency was significantly longer in the SBS group (149 days) than in the SIS group (75 days; P=0.02). The median overall survival did not significantly differ between the two groups (SBS vs. SIS, 155 days vs. 143 days; P>0.05).Conclusions: Percutaneous transhepatic bilateral stenting using either the SBS or SIS technique is safe and effective in the management of malignant hilar biliary obstruction. However, SBS offers a quicker improvement of jaundice, a lower incidence of cholangitis after the procedure, and a longer stent patency period than SIS.
Posttraumatic stress disorder and depression of survivors 12 months after the outbreak of Middle East Respiratory Syndrome in South Korea
Authors: Park Hye Yoon , Park Wan Beom , Lee So Hee , Kim Jeong Lan , Lee Jung Jae , Lee Haewoo , Shin Hyoung-Shik 
Published: April 2020

Abstract
Background: The 2015 outbreak of Middle East Respiratory Syndrome (MERS) in the Republic of Korea is a recent and representative occurrence of nationwide outbreaks of Emerging Infectious Diseases (EIDs) . In addition to physical symptoms, posttraumatic stress disorder (PTSD) and depression are common following outbreaks of EID.
Methods: The present study investigated the long-term mental health outcomes and related risk factors in survivors of MERS. A prospective nationwide cohort study was conducted 12 months after the MERS outbreak at multi-centers throughout Korea. PTSD and depression as the main mental health outcomes were assessed with the Impact of Event Scale-Revised Korean version (IES-R-K) and the Patient Health Questionnaire-9 (PHQ-9) respectively.
Results: 42.9% of survivors reported PTSD (IES-R-K ≥ 25) and 27.0% reported depression (PHQ-9 ≥ 10) at 12 months post-MERS. A multivariate analysis revealed that anxiety (adjusted odds ratio [aOR], 5.76; 95%CI, 1.29–25.58; P = 0.021), and a greater recognition of stigma (aOR, 11.09, 95%CI, 2.28–53.90; P = 0.003) during the MERS-affected period were independent predictors of PTSD at 12 months after the MERS outbreak. Having a family member who died from MERS predicted the development of depression (aOR, 12.08, 95%CI, 1.47–99.19; P = 0.020).
Conclusion: This finding implies that psychosocial factors, particularly during the outbreak phase, influenced the mental health of patients over a long-term period. Mental health support among the infected subjects and efforts to reduce stigma may improve recovery from psychological distress in an EID outbreak.
Trans-Aortic or Pulmonary Artery-Based Drainage for Left Ventricular Unloading and Veno-Arterial Extracorporeal Life Support Management in a Porcine Cardiogenic Shock Model.
Authors: Meani Paolo , Mlcek Mikulas , Kowalewski Mariusz , Raffa Giuseppe Maria , Jiritano Federica , Matteucci Matteo , Fina Dario , Martucci Gennaro , Sels Jan Willem , Delnoij Thijs , Popkova Michaela , Pilato Michele , Arcadipane Antonio , Belohlavek Jan , Lorusso Roberto 
Published: April 2020

Abstract
The authors have requested that this preprint be withdrawn due to author disagreement.
Elucidating mitochondrial DNA-markers of Ogura-based CMS linesin Indian cauliflowers (Brassica oleracea var. botrytis L.) and their floral abnormalities due to diversity in cyto-nuclear interaction
Authors: Singh Saurabh , Bhatia Reeta , Kumar Raj , Behera Tusar K. , Kumari Khushboo , Pramanik Achintya , Ghemeray Hemant , Sharma Kanika , Bhattacharya R. C. , Dey Shyam Sundar 
Published: April 2020

Abstract
Background: Mitochondrial markers can be used to differentiate diverse mitotypes as well as cytoplasms in angiosperms. In cauliflower, cultivation of hybrids is pivotal in remunerative agriculture and cytoplasmic male sterile lines constitute an important component of the hybrid breeding. Thus, the breeders look for utilizing diverse male sterile cytoplasms in hybrid progamme. In diversifying source of male sterility,it is essential to appropriately differentiate among the available male sterile cytoplasms in cauliflower.PCR polymorphism atthe key mitochondrial genes associated with male sterility will be useful in developing mitochondria specific markers for the different male sterile cytoplasms. Also, the auto and alloplasmic cytonuclear combinations result in complex floral abnormalities.Thus, the study aimed at developing mitotype specific markers of the sterile cytoplasms and to unravel thegenetic effects of the cytonuclear interactions on flower morphology in Indian cauliflowers.Results: In PCR based analysis using a set of primers targeted to orf-138, 76 Indian cauliflower lines showed presence of Ogura cytoplasm though amplicons showed polymorphism within the ofr-138 sequence. The polymorphic loci were found to be spanning over 200-280 bp and 410-470bp genomic regions of BnTR4 and orf125, respectively. Sequence analysis revealed that such cytoplasmic genetic variations could be due to single nucleotide polymorphisms and insertion or deletions of31/51 nucleotides.The cytoplasmic effects on varying nuclear-genetic backgrounds led to varying degree of floral malformations ranging from reduction in flower size, stamens and style length, modification in position of styleand anthers, absence of non-functional stamens to other floral abnormalities. These floral malformations caused dysplasia of flower structure affecting female fertility and inefficient nectar production.Conclusions: The mitochondria specific markers can distinguish ogurabased male sterile cytoplasm. Large number of Indian cauliflower lines showed mitotype variations even within the ogura based cytoplasm. Variable nucleo-cytoplasmic interactions resulted into diverse type of floral malformationsin addition to pollen sterility even within the group of ogura based CMS lines. The finding provide important reference ameliorate understanding of mechanism of cytonuclear interactions in floral organ development in Brassicas. The study will help the breeders in selecting CMS lines without any floral abnormalities in B. oleracea.
Neurotoxicity of bupivacaine and liposome bupivacaine after sciatic nerve block in healthy and streptozotocin-induced diabetic mice
Authors: Markova Liljana , Umek Nejc , Horvat Simon , Hadžić Admir , Kuroda Max , Pintarič Tatjana Stopar , Mrak Vesna , Cvetko Erika 
Published: April 2020

Abstract
Background

Diabetes mellitus and the associated neuropathic complications have become a steadily increasing global health burden. Diabetic patients are estimated to require surgery at least twice as often as nondiabetic patients. Neuropathy may change the way nerves respond to nerve blocks. There is currently no consensus on whether regional anaesthesia techniques should be adopted in these patients. Long-acting local anaesthetics ( e.g . bupivacaine HCl) or sustained-release formulations of bupivacaine ( e.g . liposomal bupivacaine) could prove neurotoxic in the presence of diabetic neuropathy. The aim of the study was to assess neurotoxicity of liposome bupivacaine in streptozotocin (STZ)-induced diabetic mice after sciatic nerve block using a reduction in fibre density and decreased myelination assessed by G-ratio as an indicator of local anaesthetic neurotoxicity.

Results

Prior to performing sciatic nerve block, higher levels of fasting glucose were recorded in diabetic mice compared to nondiabetic mice ( P < 0.001). Likewise, significant differences were noted in the tail flick and plantar test thermal latencies between the groups ( P < 0.001) which confirmed the presence of peripheral sensory neuropathy in diabetic mice. In both, diabetic and nondiabetic mice, sciatic nerve block with 0.25% bupivacaine HCl resulted in a significantly greater G-ratio (axon diameter/large fibre diameter) and an axon diameter compared to nerves treated with 1.3% liposomal bupivacaine or saline (0.9% sodium chloride) ( P < 0.01 ). Moreover, sciatic nerve block with 0.25% bupivacaine HCl resulted in higher fibre density and large fibre and axon diameters compared to the control (untreated) sciatic nerves in both STZ-induced diabetic ( P < 0.05 ) and nondiabetic mice ( P < 0.01 ). No evidence of acute or chronic inflammation was observed in any of the treatment groups.

Conclusions

Under the conditions of this study, sciatic nerve block with bupivacaine HCl, but not liposome bupivacaine or saline, resulted in histomorphometric indices of neurotoxicity. The presence of diabetes did not appear to affect the severity of the histologic findings.
Negative self-beliefs mediate the relationship between depression and suicide ideation among the elderly population: a cross-sectional study
Authors: Cheng Xinfeng , Bu He , Duan Wenjie 
Published: April 2020

Abstract
Background High suicide risk among the aged population is a growing concern. Research has linked depression symptoms to suicidal ideation in the general population. The present study examines whether negative self-beliefs mediate the cross-sectional association between depression symptoms and suicide ideation. Methods A sample of 67 elderly participants from nursing homes, ranging in age from 62 to 91 years ( M = 80.00, SD = 6.67 years; three participants did not indicate the age), completed the Negative Self Belief Inventory, the Geriatric Suicide Ideation Scale, and the 7-item depression subscale of the Depression Anxiety Stress Scale. Results The results show that the associations between depression symptom, negative self-beliefs and suicide ideation were positive ( r = .44 - .52, p < .001). Depression symptoms contributed another 13.70% explained variances. It independently explained another 5.7% variances beyond other variables to suicide ideation after the entering of negative self-belief ( B = 0.126, t = 2.183, p < .05). The indirect effect of depression symptoms on suicide ideation through negative self-belief was significant (Effect = 0.223, 95% BCa CI = [0.034, 0.468]). Conclusion Depression predicts suicide ideation via negative self -beliefs. The current study, incorporated theoretical assumptions and empirical findings in the context of Chinese culture into western prevalent suicide theories. Treatments focusing on restructuring negative self-beliefs are promising to reduce suicide in older adults in China. Future studies should test whether the mechanism in our study can transcend national and cultural boundaries.
Traditional Chinese medicine for ischemic heart disease: clinical manifestations and objective indicators
Authors: Liao Jiangquan , Tian Jiaxing , Shao Mingjing , Wang Zhe , Wei Kangkang , Chang Jiangmeng , Zhang Xiaoqiong , Chen Ming , Li Xianlun , Du Jinhang 
Published: April 2020

Abstract
Background: The clinical practice of Traditional Chinese medicine (TCM) has a history of more than 2000 years. Modern clinical trials and experimental researches of TCM have been conducted for decades and provided support for the application of TCM in the prevention and treatment of ischemic heart disease (IHD). However the level of evidence and the proper application of TCM were still barely satisfactory.Methods: In this study, we divided IHD into 5 different stages, including stable angina, unstable angina, acute myocardial infarction, post myocardial infarction and chronic heart failure. Then we systematically reviewed and meta-analyzed the existing RCTs on both clinical manifestations and objective indicators, in these 5 aspects.Results: The results indicate that TCM can both improve the clinical manifestations and ameliorate the objective parameters in different courses of IHD. Some of the improvements lead to potential long-term benefits.Conclusions: TCM is effective on CVD in different stages of diseases, both in improving clinical manifestations and objective indicators. To acquire more solid and comprehensive evidence of TCM in treating CVD, more rigorously designed RCTs with longer follow-up duration are warranted.