bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Proteomic Analysis Reveals Novel Insights into the Interaction between Rice and Xanthomonas oryzae pv. oryzae
Authors: Zhang Fan , Zhang Fan , Huang Liyu , Zeng Dan , Cruz Casiana Vera , Li Zhikang , Zhou Yongli 
Published: July 2020

Abstract
Background: Bacterial blight, which is caused by Xanthomonas oryzae pv. oryzae (Xoo), is a devastating rice disease worldwide. Rice introgression line H471, derived from the recurrent parent Huang-Hua-Zhan (HHZ) and the donor parent PSBRC28, exhibits broad-spectrum resistance to Xoo, including to the highly virulent Xoo strain PXO99A, whereas its parents are susceptible to PXO99A. To characterize the responses to Xoo, we compared the proteome profiles of the host and pathogen in the incompatible interaction (H471 inoculated with PXO99A) and the compatible interaction (HHZ inoculated with PXO99A).Results: In this study, a total of 374 rice differentially abundant proteins (DAPs) and 117 Xoo DAPs were detected in the comparison between H471 + PXO99A and HHZ + PXO99A. Most of the Xoo DAPs related to pathogen virulence, including the outer member proteins, type III secretion system proteins, TonB-dependent receptors, and transcription activator-like effectors, were less abundant in the incompatible interaction than in the compatible interaction. The rice DAPs were mainly involved in secondary metabolic processes, including phenylalanine metabolism and the biosynthesis of flavonoids and phenylpropanoids. Additionally, some DAPs involved in the phenolic phytoalexin and salicylic acid (SA) biosynthetic pathways accumulated much more in H471 than in HHZ after the inoculation with PXO99A, suggesting that phytoalexin and SA production was induced faster in H471 than in HHZ.Conclusions: Overall, our results indicate that during an incompatible interaction between H471 and PXO99A, rice plants prevent pathogen invasion and also initiate multi-component defense responses that inhibit disease development.
De novo sequencing and analysis of the transcriptome of two highbush blueberry (V. corymbosum L.) cultivars ‘Bluecrop’ and ‘Legacy’ at harvest and following post-harvest storage
Authors: Concepcion Maria Carcamo de la , Sargent Daniel James , Surbanovski Nada , Colgan Richard , Moretto Marco 
Published: July 2020

Abstract
Background: Fruit firmness and in particular the individual components of texture and moisture loss, are considered the key quality traits when describing blueberry fruit quality, and whilst these traits are genetically regulated, the mechanisms governing their control are not clearly understood. In this investigation, RNAseq was performed on fruits of two blueberry cultivars with very different storage properties, ‘Bluecrop’ and ‘Legacy’, at harvest, three weeks storage in a non-modified environment at 4 oC and after three weeks storage at 4 oC followed by three days at 21 oC, with the aim of understanding the transcriptional changes that occur during storage in cultivars with very different post-harvest fruit quality.Results: De novo assemblies of the transcriptomes of the two cultivars were performed separately and a total of 39,335 and 41,896 unigenes for ‘Bluecrop’ and ‘Legacy’ respectively were resolved. Differential gene expression analyses were grouped into four cluster profiles based on changes in transcript abundance between harvest and 24 days post-harvest. A total of 264 unigenes were up-regulated in ‘Legacy’ and down-regulated in ‘Bluecrop’, 103 were down-regulated in ‘Legacy’ and up-regulated in ‘Bluecrop’, 43 were up-regulated in both cultivars and 355 were down-regulated in both cultivars between harvest and 24 days post-harvest. Unigenes showing significant differential expression between harvest and following post-harvest cold-storage were grouped into classes of biological processes including stress responses, cell wall metabolism, wax metabolism, calcium metabolism, cellular components, and biological processes.Conclusions: In total 21 differentially expressed unigenes with a putative role in regulating the response to post-harvest cold-storage in the two cultivars were identified from the de novo transcriptome assemblies performed. The results presented provide a stable foundation from which to perform further analyses with which to functionally validate the candidate genes identified, and to begin to understand the genetic mechanisms controlling changes in firmness in blueberry fruits post-harvest.
Comparison of renal safety of Telbivudine and Entecavir in Chronic Hepatitis B patients with renal impairment: A meta-analysis
Authors: Huang Birong , Ma Shasha , Wu Jizhou 
Published: July 2020

Abstract
Background:This study aimed to evaluate the renal safety of telbivudine(LdT) and entecavir(ETV) in chronic hepatitis B (CHB) patients with renal impairment.Methods:Studies published from January 1, 2010 to February 1, 2020 were identified using the PubMed, Web of Science, Scopus,Cochrane Library,ClinicalTrials.gov and CNKI (China National Knowledge Infrastructure).Finally,a total of 7 studies (1088 patients) with eGFR outcomes were retrieved and analyzed.The meta-analysis was conducted using RevMan 5.3.Results:The results of the 7 eligible studies analyzed suggested that the eGFR was both improved after LdT and ETV treatment.Compared with the baseline level,the eGFR was significantly improved with LdT (7.02 mL/min/1.73 m2 ) while slightly improved with ETV (1.72 mL/min/1.73 m2 ) after 1 years of treatment. The eGFR was significantly higher in the LdT therapy group than in the ETV group after 6 months(RR = 4.63, 95%CI: 0.73–8.54,Z = 2.33; P = 0.02)，1 year(RR = 3.35, 95%CI: 1.18–5.52,Z = 3.02; P = 0.002)，and 2 years(RR = 11.00, 95%CI: 4.84–17.15,Z = 3.50; P = 0.0005) of treatment . Conclusion: Our meta-analysis of current evidence demonstrated that in CHB patients with impaired renal function, LdT could be the better choice than ETV.
Impact of quarantine due to COVID-19 on female urinary incontinence during exercise in CrossFit practitioners: an observational study
Authors: de Araujo Maíta Poli , Brito Luiz Gustavo Oliveira , Ejnisman Benno , Sartori Marair Gracio Ferreira , Girão Manoel B.C. , Pochini Alberto de Castro , Group Cross Continence Brazil 
Published: July 2020

Abstract
Introduction and hypothesis: Urinary incontinence (UI) during exercise (athletic incontinence) affects about 30% of CrossFit practitioners. Due to the COVID-19 pandemic, CrossFit academies were closed, impacting on several dimensions of the health of these athletes. We aimed to evaluate the effect of quarantine due to COVID19 pandemic on the training volume and UI during exercises for female crossfitters. Methods: A cross-sectional study was performed among 197 female CrossFit practitioners. An online questionnaire was emailed containing questions about frequency, duration and intensity of training and data related to the COVID-19 pandemic. Whether UI stopped among participants, they were asked about the possible reasons why this happened. A 5% significance level was stablished.Results: Mean age of the participants was 32 years old, with an average frequency of training of 50 minutes per day, four times/ week. Most participants lived in an apartment (65.5%) and with another person (40.1%). Main CrossFit training location during quarantine was inside home (55%). There was a decrease in training intensity in 64% of the respondents. Exercises with their own body weight, such as air squat (98.2%) followed by push up (92.2%) were the most performed. UI was reported by 32% of participants before the COVID-19 pandemic, and only 14% of them during the pandemic (OR = 0.32[0.19-0.53],p <0.01; univariate analysis). Practitioners reported that the reason possibly related to UI improvement was the reduction of training intensity and not performing double under exercise. Conclusion: Quarantine by COVID-19 reduced in 18% of UI during exercises by CrossFit practitioners.
The pattern of hospital-community-home (HCH) nursing in tracheostomy patients with severe traumatic brain injury: is it feasible?
Authors: Yin Xiangyi , Wu Jie , Zhou Lihui , Ni Chunyan , Xiao Minyan , Meng Xianlan , Zhu Xiaowen , Cao Qing , Li Huifen 
Published: July 2020

Abstract
Background
Tracheostomy is very common in patients with severe traumatic brain injury (TBI), and long-term nursing care are needed for those patients. We aimed to evaluate the effects of hospital-community-home (HCH) nursing in those patients.
Methods
Tracheostomy patients with severe TBI were included. Glasgow coma score (GCS), Karnofsky, Self-Anxiety Scale (SAS) and Barthel assessment at the discharge and two months after discharge were evaluated.
Results
A total of 60 patients were included. There weren’t significant differences between two groups in the GCS, Karnofsky, SAS and Barthel index at discharge((all p > 0.05), the GCS, Karnofsky and Barthel index was all significantly increased after two months follow-up for two groups (all p < 0.05), and the GCS, Karnofsky and Barthel index at two months follow-up in HCH group was significantly higher than that of control group(all p < 0.05), but the SAS at two months follow-up in HCH group was significantly less than that of control group(p = 0.009). The incidence of block of artificial tracheal cannula and readmission in HCH group were significant less than that of control group (all p < 0.05).
Conclusion
HCH nursing care is feasible in tracheostomy patients with severe TBI, future studies are needed to further evaluate the role of HCH nursing care.
Intestinal thromboangiitis obliterans: Consequence of a delay in diagnosis
Authors: Mishra Swastik Sourav , Mishra Tushar Subhadarshan , Mitra Suvradeep , Kumar Pankaj 
Published: July 2020

Abstract
Background: Thromboangiitis obliterans (TAO) or Buerger’s disease is a form of peripheral vascular disease in young male smokers. The involvement of the intestine occurs only about 2% of the cases. Symptoms of peripheral vascular disease usually precede intestinal manifestations, although the latter can sometimes be the index presentation. The cessation of smoking usually, though not necessarily, prevents the progression of the disease and visceral involvement.Case presentation: Our patient presented with diffuse abdominal pain along with bouts of vomiting and loose motions. He was a known smoker with a prior history of amputation of the right foot, four years before. Physical examination revealed abdominal distension and diffuse tenderness and guarding. An omental band attached to the tip of the appendix was discovered at the initial exploration along with dilated proximal bowel loops, for which a release of the omental band along with appendectomy was done. He developed an enterocutaneous fistula on the sixth postoperative day for which the abdomen was re-explored which revealed multiple segmental perforation in the jejunum and two subcentimetric perforation adjacent to each other in the distal ileum. Resection of the affected jejunal segment was performed followed by Roux-en-Y gastrojejunosotomy and distal ileostomy. A feeding jejunostomy was also performed. The bleeding from the cut ends was unsatisfactory. The patient however had persistent feculent discharge from the wound for which a third exploration was done which revealed a leak from the gastrojejunostomy and feeding jejunosotomy sites, both of which were repaired primarily. However, the patient developed septicemia with persistently elevated serum lactate levels which progressed to refractory septic shock and ultimately succumbed to the illness on 23rd postoperative day of the index surgery.Conclusion: Acute abdomen in a young man, who is a chronic smoker and having an antecedent history of amputation of some part of an extremity for a nontraumatic cause should raise the suspicion of Buerger’s disease of the intestine. Although it is a progressive disease and things are already late by the time intestinal symptoms manifest, early detection may give some scope of salvage and decreasing morbidity and mortality.
Association of Iodine-Related Knowledge, Attitudes, and Behaviours with Urinary Iodine Excretion in Pregnant Women with Mild Iodine Deficiency
Authors: Wang Zhengyuan , Wu Yiwen , Shi Zehuan , Song Jun , Wang Guoquan , Song Qi , Jin Wei , Cui Xueying , Wu Chunfeng , Zang Jiajie , Guo Changyi 
Published: July 2020

Abstract
Background: China’s universal salt-iodization program has all but eliminated iodine deficiency disorders. Concern has shifted to mild iodine deficiency. Our study examined factors with the potential to predict mild iodine deficiency in pregnant women. Methods: A total of 2 400 pregnant women were enrolled using a multistage, stratified, random-sampling method. Data were collected through face-to-face interviews, a standardized questionnaire, an iodine-related knowledge questionnaire, urine samples, and household cooking salt samples. Results: The median urinary iodine concentration (MUIC) was 148.0 μg/L for all participants, and 155.0 μg/L, 151.0 μg/L, and 139.6 μg/L in the first, second, and third trimesters, respectively. The third trimester’s MUIC was significantly lower than that of the first trimester, and the usage rates of iodized salt and qualified-iodized salt were 71.5% and 59.4%, respectively. Iodine-related knowledge was significantly different between the high and low UIC groups. Participants’ MUIC increased significantly with increases in iodine-related knowledge. The third trimester was a significant risk factor for high UIC, whereas abundant iodine-related knowledge, study the dietary knowledge urgently, and consumption of iodine-rich food within 48 hours of a urine iodine test were significant protective factors for high UIC (P<0.05). Conclusions: Iodine levels are adequate among pregnant women in Shanghai during the first and second trimesters, but insufficient in the third trimester. The use of iodized cooking salt does not determine the iodine status of pregnant women. Abundant iodine-related knowledge is important for pregnant women in the third trimester to maintain adequate urinary iodine.
Targeting Phosphoglycerate Dehydrogenase in Multiple Myeloma
Authors: Elsaadi Samah , Steiro Ida , Abdollahi Pegah , Vandsemb Esten N. , Yang Rui , Slørdahl Tobias S. , Rø Torstein Baade , Menu Eline , Sponaas Anne-Marit , Børset Magne 
Published: July 2020

Abstract
Background: Multiple myeloma (MM) is a hematological malignancy characterized by the clonal expansion of plasma cells in the bone marrow. To date, this disease is still incurable and novel therapeutic approaches are required. Phosphoglycerate dehydrogenase (PHGDH) is the first and rate-limiting enzyme in the de novo serine synthesis pathway, and it has been attributed to bortezomib-resistance in MM.Methods: Two different PHGDH inhibitors, CBR5884 and NCT-503, were tested against human myeloma cell lines, primary MM cells from patients, and peripheral blood mononuclear cells isolated from healthy donors. The PHGDH inhibitors were then tested in combination with proteasome inhibitors in different MM cell lines, including proteasome-resistant cell lines. Furthermore, we confirmed the effects of PHGDH inhibition through knocking down PHGDH and the effect of NCT-503 in vivo in the 5T33MM mouse model.Results: All the tested myeloma cell lines expressed PHGDH and were sensitive to doses of NCT-503 that were tolerated by peripheral blood mononuclear cells isolated from healthy donors. Upon testing bortezomib in combination with NCT-503, we noticed a clear synergy in several HMCLs. The sensitivity to bortezomib also increased after PHGDH knockdown, mimicking the effect of NCT-503 treatment. Interestingly, targeting PHGDH reduced the intracellular redox capacity of the cells. Furthermore, combination treatment with NCT-503 and bortezomib exhibited a therapeutic advantage in vivo. Conclusions: Our study shows the therapeutic potential of targeting PHGDH in MM, and suggest it as a way to overcome the resistance to proteasome inhibitors.
Propofol Attenuates Lung but not Brain Cancer Cell Malignancy through Metabolism and Cell Signaling Modulation&nbsp;In Vitro
Authors: Hu Cong , Iwasaki Masae , Liu Zhigang , Wang Bincheng , Li Xiaomeng , Lin Han , Li Jun , Li Jia V. , Lian Qingquan , Ma Daqing 
Published: July 2020

Abstract
Background: Intravenous anesthesia with propofol was reported to improve cancer surgical outcomes when compared with inhalational anesthesia. However, the underlying molecular mechanisms largely remain unknown. The current study aims to investigate whether propofol affects cancer cell biology including tumor metastasis-related gene expression, cellular signaling and metabolic changes in lung and brain cancer cells.Methods: Lung cancer (A549) or neuroglioma (H4) cells were treated with propofol at a clinically relevant concentration (4 μg/mL) for 2 hours, followed by 24 hours recovery. Tumor metastasis-related gene expressions were assessed using a PCR array and validated with qRT-PCR. Glucose transporter 1 (GLUT1), brain protein 44-like (BRP44L), pigment epithelium-derived factor (PEDF), Akt, phospho-Akt (p-Akt), extracellular-signal-regulated kinase 1/2 (Erk1/2), phospho-Erk1/2 (p-Erk1/2), and hypoxia-inducible factor 1 alpha (HIF-1α) expressions were determined using immunofluorescent staining and/or western blotting. The metabolites in cell extract and media following propofol treatment were characterized using proton nuclear magnetic resonance ( 1 H NMR) spectroscopy. The malignant hallmarks including cell viability, proliferation, migration, and invasion were evaluated using cell counting kit-8 (CCK-8) assay, Ki-67 staining, wound healing and transwell assay, respectively.Results: Propofol reduced cell viability and inhibited cell proliferation, migration and invasion of lung cancer cells, but not neuroglioma cells. In lung cancer cells, gene expressions of VEGFA, CTBP1, CST7, CTSK, CXCL12, and CXCR4 were downregulated, while NR4A3, RB1, NME1, MTSS1, NME4, SYK, APC, and FAT1 were upregulated following the propofol treatment. Furthermore, propofol downregulated GLUT1, BRP44L, p-Akt, p-Erk, and HIF-1α expressions in lung cancer cells and upregulated PEDF expression. Propofol increased glutamate and glycine but decreased acetate and formate in lung cancer cells whilst increased lactate, valine, isoleucine, and leucine and glycerol, and decreased pyruvate and isopropanol in the culture media. Consistent with the phenotypical changes, these molecular and metabolic changes were not observed in the neuroglioma cells.Conclusions: Our findings indicated “anti-tumor” effects of propofol on the lung cancer but not neuroglioma, through the regulation of tumor metastasis-related genes, multi-cellular signaling and cellular metabolism.
Treatment of cauda equina syndrome due to lumbar disc herniation&nbsp;with&nbsp;percutaneous endoscopic lumbar discectomy
Authors: He Hai-Chao , Zhang Yong-jin 
Published: July 2020

Abstract
Background: Throughout the last decades, endoscopic techniques to treat lumbar disc herniation (LDH) have gained popularity in clinical practice. However, there is little literature on the treatment of Cauda Equina Syndrome (CES) due to lumbar disc herniation with percutaneous endoscopic lumbar discectomy (PELD). This study aims to evaluate the feasibility and clinical efficacy of PELD for CES caused by disc herniation.Methods: Between October 2012 and April 2017, 15 patients CES caused by LDH at the early and intermediate stages of Shi’s classification were selected as the objects of study, who underwent PELD. All patients were followed up for at least two years. The patients’ lumbar pain and limb pain were evaluated using visual analogue scale (VAS) scores. Patient's satisfaction was evaluated using the MacNab outcome scale. Clinical outcomes were measured preoperatively, at 3 days, 3 months, 6 month and the last follow-up.Results: The VAS score for lumbar pain was 6.67±1.05 preoperatively and 3.27±0.96, 2.73±0.46, 2.40±0.51, and 1.80±0.41, at 3 days, 3 months, 6 months and the last follow-up postoperatively respectively. The VAS score for limb pain was 7.13±1.19 preoperatively and 2.93±0.80, 2.60±0.51, 2.20±0.56, and 1.47±0.52, at 3 days, 3 months, 6 months and the last follow-up postoperatively respectively. These postoperative scores were all significantly different when compared with preoperative scores (P < 0.01). According to the modified MacNab outcome scale, excellent was obtained in 8 of 15 patients, good was obtained in 5 patients, and fair was obtained in 2 patients, and 86.67% of these patients had excellent and good outcomes at the final follow-up. Complications included one patient of cerebrospinal fluid leakage, and one patient developed recurrent herniation and who finally acquired satisfactory result after reoperation.Conclusion: PELD could be used as an alternative surgical method for the treatment of CES due to lumbar disc herniation in properly selected cases and appropriate patient selection. However, the operator should pay attention to foraminoplasty to enlarge the working space.
Knowledge, Attitude, and Practice of Nurses Towards the Prevention and Control of COVID-19
Authors: Jin Zhengjiang , Luo Lan , Lei Xinyun , Zhou Wen , Wang Zhenhui , Yi Lu , Liu Nian 
Published: July 2020

Abstract
Background: Coronavirus disease 2019(COVID-19) has been spreading globally, with severe impacts on health and economies. Nursing is an essential component of medical care, and nurses’ knowledge, attitude, and practice (KAP) about the prevention and control of the disease directly affects patient’s outcomes ,but there is little know about the nurses’ KAP during the COVID-19 epidemic.Methods: An anonymous survey was conducted using customized questionnaires designed by Sojump platform. 1323 nurses in Wuhan were the survey object for the KAP survey. The survey included 48 questions regarding the basic information of nurses and knowledge, attitude, and prevention measures for COVID-19. Results: A total of 1323 nurses in Wuhan were included in the survey, of whom women (95.7%) under 40 years old (94.6%) accounted for the majority. Nurses have a comprehensive knowledge of problems such as the COVID-19 susceptible population, isolation ward layout process, environmental cleaning and disinfection, hand hygiene measures, infectious disease case reporting processes, standard prevention measures, and personal protection level, with more than 60% accuracy. However, they have a general lack of knowledge about the route of transmission of COVID-19, the use of protective equipment, patient management, medical waste disposal, and occupational exposure emergency response, with less than 45% accuracy. There was a gap between the attitude and the practice of nurses (p <0.05). Moreover, the knowledge level of nurses has an impact on their attitude (OR 1.52, 95% CI 1.10-2.08, p = 0.008), and title (OR 1.48, 95% CI 1.01-2.15, p = 0.042), place of work (OR 1.59, 95% CI 1.36-1.85, p <0.001), and attitude (OR 1.93, 95% CI 1.52-2.46, p <0.001) had an impact on their practice.Conclusion: The nurses in Wuhan during the epidemic have insufficient knowledge about COVID-19 infection prevention and control, and there are gaps in attitude and practice, which further affect their attitude and practice. It is therefore necessary to strengthen relevant knowledge training on COVID-19 among nurses.