bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineering oleaginous yeast Yarrowia lipolytica for enhanced limonene production from xylose and lignocellulosic hydrolysate
Authors: Yao Feng , Liu Shun-Cheng , Wang Dan-Ni , Liu Zhi-Jie , Hua Qiang , Wei Liu-Jing 
Published: August 2020

ABSTRACT
Limonene, a valuable cyclic monoterpene, has been broadly studied in recent decades due to its wide application in the food, cosmetics and pharmaceutical industries. Engineering of the yeast Yarrowia lipolytica for fermentation of renewable biomass lignocellulosic hydrolysate may reduce the cost and improve the economics of bioconversion for the production of limonene. The aim of this study was to engineer Y. lipolytica to produce limonene from xylose and low-cost lignocellulosic feedstock. The heterologous genes XR and XDH and native gene XK encoding xylose assimilation enzymes, along with the heterologous genes tNDPS1 and tLS encoding orthogonal limonene biosynthetic enzymes, were introduced into the Po1f strain to facilitate xylose fermentation to limonene. The initially developed strain produced 0.44 mg/L of limonene in 72 h with 20 g/L of xylose. Overexpression of genes from the mevalonate pathway, including HMG1 and ERG12, significantly increased limonene production from xylose to ∼9.00 mg/L in 72 h. Furthermore, limonene production peaked at 20.57 mg/L with 50% hydrolysate after 72 h when detoxified lignocellulosic hydrolysate was used. This study is the first to report limonene production by yeast from lignocellulosic feedstock, and these results indicate the initial steps toward economical and sustainable production of isoprenoids from renewable biomass by engineered Y. lipolytica.
Who's Out of Touch? Media Misperception of Public Opinion on US Foreign Policy
Authors: Gift Thomas , Monten Jonathan 
Published: August 2020

Abstract
Although experts in the United States are often criticized as being “out of touch” for failing to understand the political views of average Americans, arguably no group has been more susceptible to this charge than the media. In this article, we exploit unique paired surveys to measure how accurately US foreign policy media experts assess public opinion compared to other foreign policy experts on the critical issue of American engagement in the world. We find that while experts, on average, substantially underestimate how favorable US citizens are toward international engagement, the media is more inaccurate than other types of experts. We suggest potential reasons for these findings that may serve as the basis for future research. Overall, our study contributes to a growing literature on elite misperceptions of the public and underscores the particular inaccuracies of the media in understanding the attitudes of Americans.
Educational Differences in Decline in Maximum Gait Speed in Older Adults Over an 11-Year Follow-up
Authors: Kyrönlahti Saila M , Stenholm Sari , Raitanen Jani , Neupane Subas , Koskinen Seppo , Tiainen Kristina 
Published: August 2020

Abstract

Background
This study examined educational differences in decline in maximum gait speed over an 11-year follow-up in the general Finnish population aged 55 years and older and assessed the contribution of lifestyle factors, body mass index, physical workload, and chronic conditions on the association.

Method
Data from the nationally representative Health 2000 Survey and its 11-year follow-up were used. Participants aged 55 years and older with maximum gait speed measured at both time points were included (n = 1128). Information on education, age, sex, lifestyle factors, body mass index, physical workload, and chronic conditions was collected at baseline. General linear model was used to examine differences in decline in maximum gait speed between education groups. Mediation analyses using the product method was conducted to partition the total effect of education on decline in maximum gait speed into direct effect and indirect effect acting through mediators.

Results
Decline in maximum gait speed was greater in low and intermediate education groups in comparison to the high education group (0.24 m/s [95% confidence interval 0.21–0.26], 0.24 m/s [0.21–0.28], 0.10 m/s [0.07–0.14], respectively]. The most important mediators were higher body mass index and lifetime exposure to physical workload among the less educated, accounting for 10% and 11% of the total effect, respectively.

Conclusions
Education-based disparities in objectively measured mobility increase with age as lower education is associated with greater decline in gait speed. Higher body mass index and physical workload among less educated contributed most to the educational disparities in age-related decline in maximum gait speed.
Slip distribution of the 2005 Nias earthquake (Mw 8.6) inferred from geodetic and far-field tsunami data
Authors: Fujii Yushiro , Satake Kenji , Watada Shingo , Ho Tung-Cheng 
Published: August 2020

SUMMARYWe estimated the slip distribution on the fault of the 2005 Nias earthquake (Mw 8.6) by inversions of local GPS and coastal uplift/subsidence data and tsunami waveform data. The 2005 Nias earthquake occurred approximately three months after the 2004 Sumatra–Andaman earthquake (Mw 9.1) at the southern extension off Sumatra Island, Indonesia. The tsunami from the 2005 earthquake caused significantly less damage than the 2004 tsunami, yet was recorded at tide gauges and ocean bottom pressure gauges around the Indian Ocean, including the coasts of Africa and Antarctica. The elastic and gravitational coupling between the solid earth and the ocean causes not only a traveltime delay but also the change of waveforms of far-field tsunamis relative to the prediction based on the long-wave theory. We corrected the computed tsunami Green's functions for the elastic and gravitational coupling effect in the tsunami waveform inversion. We found a diffused slip (∼2 m over an area of 400 km × 100 km) at deeper parts (20–54 km) of the fault with a large localized slip (7 m over 100 km × 100 km) slightly south of the epicentre. The large slips at deeper parts of the fault were responsible for the small tsunami generation. Inversion using far-field tsunami data yielded a slip distribution similar to that obtained using local geodetic data alone and that from the joint inversion of local geodetic and far-field tsunami data, which is also similar to slip distributions from previous studies based on local geodetic data. This demonstrates that far-field tsunami waveforms, once corrected for propagation effects, can be used to estimate the slip distribution of large submarine earthquakes leading to results that are similar to those obtained using sparse local geodetic data.
Nodal Solutions for Sublinear-Type Problems with Dirichlet Boundary Conditions
Authors: Bonheure Denis , Moreira dos Santos Ederson , Parini Enea , Tavares Hugo , Weth Tobias 
Published: August 2020

Abstract
We consider nonlinear 2nd-order elliptic problems of the type $$\begin{align*} & -\Delta u=f(u)\ \textrm{in}\ \Omega, \qquad u=0\ \textrm{on}\ \partial \Omega, \end{align*}$$where $\Omega $ is an open $C^{1,1}$–domain in ${{\mathbb{R}}}^N$, $N\geq 2$, under some general assumptions on the nonlinearity that include the case of a sublinear pure power $f(s)=|s|^{p-1}s$ with $0&lt;p&lt;1$ and of Allen–Cahn type $f(s)=\lambda (s-|s|^{p-1}s)$ with $p&gt;1$ and $\lambda&gt;\lambda _2(\Omega )$ (the second Dirichlet eigenvalue of the Laplacian). We prove the existence of a least energy nodal (i.e., sign changing) solution and of a nodal solution of mountain-pass type. We then give explicit examples of domains where the associated levels do not coincide. For the case where $\Omega $ is a ball or annulus and $f$ is of class $C^1$, we prove instead that the levels coincide and that least energy nodal solutions are nonradial but axially symmetric functions. Finally, we provide stronger results for the Allen–Cahn type nonlinearities in case $\Omega $ is either a ball or a square. In particular, we give a complete description of the solution set for $\lambda \sim \lambda _2(\Omega )$, computing the Morse index of the solutions.
Boosting Union Membership: Reconciling Liberal and Social Democratic Conceptions of Freedom of Association via a Union Default
Authors: Harcourt Mark , Gall Gregor , Novell Nisha , Wilson Margaret 
Published: August 2020

Abstract
Deploying insights from legal experts in New Zealand, this article examines how a union default could strengthen unions and boost their membership, while reconciling conflicting liberal and social democratic conceptions of freedom of association. The insights are used to develop a broad framework by which a default system could work both in New Zealand and more generally. These insights are then applied to the case of Britain, wherein components of the institutional architecture, embodied in Schedule A1 of the Employment Relations Act 1999, could be appropriately amended.
Critical Role of Innate Immunity to Flagellin in the Absence of Adaptive Immunity
Authors: Zou Jun , Zhao Xu , Shi Zhenda , Zhang Zhan , Vijay-Kumar Matam , Chassaing Benoit , Gewirtz Andrew T 
Published: August 2020

Abstract

Background
Bacterial flagellin is a major target of innate and adaptive immunity, both of which can promote and/or compensate for deficiencies in each other’s function.

Methods
To investigate the role of innate immune detection of flagellin irrespective of adaptive immunity, we examined the consequences of loss of Toll-like receptor 5 (T5) and/or Nod-like receptor 4 (N4) upon a Rag1-deficient background.

Results
Mice lacking Toll-like receptor 5 and Rag1 (T5/Rag-DKO) exhibited frequent lethal Pasteurellaceae-containing abscesses that prevented breeding of these mice. Mice lacking Toll-like receptor 5, Nod-like receptor 4, and Rag1 (T5/N4/Rag-TKO) also resulted in sporadic lethal abdominal abscesses caused by similar Pasteurellaceae. In the absence of such infections, relative to Rag1-KO, T5/N4/Rag-TKO mice exhibited microbiota encroachment, low-grade inflammation, microbiota dysbiosis, and, moreover were highly prone to Citrobacter infection and developed severe colitis when adoptively transferred with colitogenic T cells. Relative proneness of T5/N4/Rag-TKO mice to T-cell colitis was ablated by antibiotics while fecal microbiota transplant from T5/N4/Rag-TKO mice to wild-type mice transferred proneness to Citrobacter infection, indicating that dysbiosis in T5/N4/Rag-TKO mice contributed to these phenotypes.

Conclusions
These results demonstrate a critical role for innate immune detection of flagellin, especially in the intestinal tract and particularly in hosts deficient in adaptive immunity.
The impact of cell maturation and tissue microenvironments on the expression of endosomal Toll-like receptors in monocytes and macrophages
Authors: Sato Ryota , Reuter Tatjana , Hiranuma Ryosuke , Shibata Takuma , Fukui Ryutaro , Motoi Yuji , Murakami Yusuke , Tsukamoto Hiroki , Yamazaki Satoshi , Liu Kaiwen , Saitoh Shin-Ichiroh , Latz Eicke , Miyake Kensuke 
Published: August 2020

Abstract
Toll-like receptors (TLRs) impact myeloid cell responsiveness to environmental cues such as pathogen components and metabolites. Although TLR protein expression in monocytes and tissue macrophages is thought to be optimized for microenvironments in each tissue, a comprehensive study has not been reported. We here examined protein expression of endogenous TLRs in tissue-resident myeloid cells. Neutrophils in peripheral blood, spleen, liver and lung expressed TLR2, TLR4 and TLR5 in all tissues. Ly6C+ MHC II‒ classical monocytes mature into Ly6C‒ MHC II+ monocyte-derived dendritic cells (moDCs) or Ly6C‒ MHC II‒ patrolling monocytes. These subsets were found in all the tissues studied. TLR2 and TLR4 were displayed on all of these subsets, regardless of location. In contrast, expression of endosomal TLRs did vary with tissues and subsets. moDCs expressed TLR9, but much less TLR7. In contrast, TLR7, not TLR3 or TLR9, was highly expressed in classical and patrolling monocytes. Tissue macrophages such as red pulp macrophages in the spleen, Kupffer cells in the liver, microglia in the brain, alveolar macrophages in the lung and adipose tissue macrophages all expressed TLR2, TLR4 and TLR3. TLR7 was also expressed in these tissue macrophages except Kupffer cells in the liver. TLR9 expression in tissue macrophages was much lower or hard to detect. These results suggest that expression of endosomal TLRs in myeloid cells is influenced by their differentiation status and tissue-specific microenvironments.
Evaluation of the SpeeDxResistancePlus®GC and SpeeDx GC 23S 2611 (beta) molecular assays for prediction of antimicrobial resistance/susceptibility to ciprofloxacin and azithromycin inNeisseria gonorrhoeae
Authors: Hadad Ronza , Cole Michelle Jayne , Ebeyan Samantha , Jacobsson Susanne , Tan Lit Yeen , Golparian Daniel , Erskine Simon , Day Michaela , Whiley David , Unemo Magnus ,   the European collaborative group, Torreblanca Raquel Abad , Ásmundsdóttir Lena Rós , Balla Eszter , De Baetselier Irith , Bercot Beatrice , Bergheim Thea , Borrego Maria José , Buder Susanne , Cassar Robert , Cole Michelle , van Dam Alje , Eder Claudia , Hoffmann Steen , Hunjak Blazenka , Jeverica Samo , Kirjavainen Vesa , Maikanti-Charalambous Panayiota , Miriagou Vivi , Młynarczyk-Bonikowska Beata , Pakarna Gatis , Pavlik Peter , Perrin Monique , Pett Joseph , Stefanelli Paola , Templeton Kate , Unemo Magnus , Viktorova Jelena , Zákoucká Hana 
Published: August 2020

AbstractBackgroundAccurate molecular assays for prediction of antimicrobial resistance (AMR)/susceptibility in Neisseria gonorrhoeae (Ng) can offer individualized treatment of gonorrhoea and enhanced AMR surveillance.ObjectivesWe evaluated the new ResistancePlus® GC assay and the GC 23S 2611 (beta) assay (SpeeDx), for prediction of resistance/susceptibility to ciprofloxacin and azithromycin, respectively.MethodsNine hundred and sixty-seven whole-genome-sequenced Ng isolates from 20 European countries, 143 Ng-positive (37 with paired Ng isolates) and 167 Ng-negative clinical Aptima Combo 2 (AC2) samples, and 143 non-gonococcal Neisseria isolates and closely related species were examined with both SpeeDx assays.ResultsThe sensitivity and specificity of the ResistancePlus® GC assay to detect Ng in AC2 samples were 98.6% and 100%, respectively. ResistancePlus® GC showed 100% sensitivity and specificity for GyrA S91 WT/S91F detection and 99.8% sensitivity and specificity in predicting phenotypic ciprofloxacin resistance. The sensitivity and specificity of the GC 23S 2611 (beta) assay for Ng detection in AC2 samples were 95.8% and 100%, respectively. GC 23S 2611 (beta) showed 100% sensitivity and 99.9% specificity for 23S rRNA C2611 WT/C2611T detection and 64.3% sensitivity and 99.9% specificity for predicting phenotypic azithromycin resistance. Cross-reactions with non-gonococcal Neisseria species were observed with both assays, but the analysis software solved most cross-reactions.ConclusionsThe new SpeeDx ResistancePlus® GC assay performed well in the detection of Ng and AMR determinants, especially in urogenital samples. The GC 23S 2611 (beta) assay performed relatively well, but its sensitivity, especially for predicting phenotypic azithromycin resistance, was suboptimal and further optimizations are required, including detection of additional macrolide resistance determinant(s).
Imaging and Management of Internal Mammary Lymph Nodes
Authors: Samreen Naziya , Dhage Shubhada , Gerber Naamit Kurshan , Chacko Celin , Lee Cindy S 
Published: August 2020

Abstract
Internal mammary lymph nodes (IMLNs) account for approximately 10%–40% of the lymphatic drainage of the breast. Internal mammary lymph nodes measuring up to 10 mm are commonly seen on high-risk screening breast MRI examinations in patients without breast cancer and are considered benign if no other suspicious findings are present. Benign IMLNs demonstrate a fatty hilum, lobular or oval shape, and circumscribed margins without evidence of central necrosis, cortical thickening, or loss of fatty hilum. In patients with breast cancer, IMLN involvement can alter clinical stage and treatment planning. The incidence of IMLN metastases detected on US, CT, MRI, and PET-CT ranges from 10%–16%, with MRI and PET-CT demonstrating the highest sensitivities. Although there are no well-defined imaging criteria in the eighth edition of the American Joint Committee on Cancer Staging Manual for Breast Cancer, a long-axis measurement of ≥ 5 mm is suggested as a guideline to differentiate benign versus malignant IMLNs in patients with newly diagnosed breast cancer. Abnormal morphology such as loss of fatty hilum, irregular shape, and rounded appearance (which can be quantified by a short-axis/long-axis length ratio greater than 0.5) also raises suspicion for IMLN metastases. MRI and PET-CT have good sensitivity and specificity for the detection of IMLN metastases, but fluorodeoxyglucose avidity can be seen in both benign conditions and metastatic disease. US is helpful for staging, and US-guided fine-needle aspiration can be performed in cases of suspected IMLN metastasis. Management of suspicious IMLNs identified on imaging is typically with chemotherapy and radiation, as surgical excision does not provide survival benefit and is performed only in rare cases.
Field Efficacies and Joint Actions of Beta-cyfluthrin Mixed With Thiamethoxam or Tolfenpyrad Against Diaphorina citri (Hemiptera: Liviidae)
Authors: Tang Tao , Zhao Mingping , Wang Pei , Xiao Yi , Huang Shengkong , Fu Wei 
Published: August 2020

Abstract
The Asian citrus psyllid, Diaphorina citri Kuwayama, is the most serious pest of citrus because it is a vector for the highly destructive citrus greening disease (huanglongbing, HLB). Currently, insecticide applications are being used widely to control psyllid populations, thereby suppressing the spread of HLB. In the present study, topical application bioassays were performed to detect the joint actions of beta-cyfluthrin and thiamethoxam or tolfenpyrad against D. citri adults in the laboratory. In 2019, a field plot experiment was conducted to evaluate the control efficacies of beta-cyfluthrin+thiamethoxam 22% capsule suspension and beta-cyfluthrin+tolfenpyrad 30% microemulsion against D. citri using foliar sprays. For the former, a 9:13 mass ratio had the highest synergistic effect, with a cotoxicity coefficient of 188.64. For the latter, a 5:25 mass ratio had the highest synergistic effect, with a cotoxicity coefficient of 153.94. A one-time foliar spray of the former at 30–40 mg/kg or of the latter at 40–60 mg/kg effectively controlled D. citri, with control efficacies varying from 80.1 to 99.4% or 80.4 to 100.0%, during the 3–30 d after treatment, respectively. Moreover, field observations indicated that these foliar sprays at the tested rates had no negative effects on citrus trees. Thus, foliar sprays of beta-cyfluthrin+thiamethoxam or beta-cyfluthrin+tolfenpyrad under the given conditions may control D. citri.
Comparative Performance of the Fall Armyworm (Lepidoptera: Noctuidae) Reared on Various Cereal-Based Artificial Diets
Authors: Jin Tao , Lin Yu-ying , Chi Hsin , Xiang Kai-ping , Ma Guang-chang , Peng Zheng-qiang , Yi Ke-xian 
Published: August 2020

AbstractThe fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera, Noctuidae), is a polyphagous and highly destructive agricultural pest that invaded mainland China in 2019. To facilitate research on this pest, it is important to formulate and formalize a suitable artificial diet based on local ingredients. In this study, the life histories of fall armyworm reared on corn leaves and four artificial diets were recorded. The four artificial diets used were: soybean and sucrose-based (SS), soybean and wheat germ-based (SW), chickpea and wheat germ-based (CPW), and corn and soybean-based (CNS). The intrinsic rates of increase were 0.1957, 0.1981, 0.1816, 0.1748, and 0.1464 per day in the fall armyworm populations fed corn leaves, CNS, SW, CPW, and SS, respectively. The highest fecundity (F = 1225.4 eggs per female) and net reproduction rate (R0 = 544.7 offspring per individual) were observed for the fall armyworm reared on the CNS diet. Moreover, the developmental rate, survival rate, and fecundity were used to calculate the projection of the population growth. Projection results showed that the fall armyworm populations can increase considerably faster when fed the CNS diet compared with the other diets. In addition, the mass-rearing system showed that the most efficient and economical strategy would be to rear the fall armyworm on the CNS diet. The results indicated that the CNS diet was the most suitable diet for the fall armyworm mass rearing.
Bottom-up Effects on Tri-trophic Interactions: Plant Fertilization Enhances the Fitness of a Primary Parasitoid Mediated by Its Herbivore Host
Authors: Pekas Apostolos , Wäckers Felix L 
Published: August 2020

AbstractPlants play a pivotal role in interactions involving herbivores and their natural enemies. Variation in plant primary and secondary metabolites not only affects herbivores but, directly and indirectly, also their natural enemies. Here, we used a commercial NPK fertilizer to test the impact of three fertilizer, namely 50, 100, and 200 ppm nitrogen, and one control (i.e., water) treatments, on the weight of the nymphs of the whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Subsequently, the whitefly parasitoid Eretmocerus mundus Mercet (Hymenoptera: Aphelinidae) was reared on the different groups of whitefly nymphs and upon parasitoid emergence, the number of oocytes was determined as a measure of reproductive capacity. Trials were done on tomato and tobacco plants. The level of nitrogen concentration in tobacco leaves was directly correlated with the fertilizer applications, thus confirming the effect of our fertilizer treatments. Both in tomato and tobacco plants, healthy as well as parasitized whitefly nymphs, were heaviest in the 200 ppm nitrogen treatment. The highest number of oocytes per female parasitoid was recorded in the 200 ppm nitrogen treatment in tomato (31% more oocytes as compared with the control) and in the 100 and 200 ppm nitrogen treatments in tobacco (200% more oocytes). We suggest that the increase in oocytes was the result of the enhanced size (food quantity) and/or nutritional quality of the whitefly host. The practical implications of these results for the mass rearing of whitefly parasitoids and for biological pest control are discussed.