bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recycling performance of graphene oxide-chitosan hybrid hydrogels for removal of cationic and anionic dyes
Authors: Vo Thi Sinh , Vo Tran Thi Bich Chau , Suk Ji Won , Kim Kyunghoon 
Published: February 2020

AbstractWater is one of the most important resources for human survival and development. Efficient wastewater treatment techniques such as coagulation, filtration, ozonation, and reverse osmosis have been studied to remove toxic materials from water. Implementation of adsorption columns has been proven to be an efficient wastewater treatment method, particularly for the removal of organic contaminants. In this study, we present the preparation of an eco-friendly graphene oxide–chitosan (GC) composite hydrogel column (GCCHC) and its application as a broad-spectrum adsorbent for wastewater treatment. The GCCHC shows a high removal capacity towards different contaminants including both cationic dyes [methylene blue (MB) and rhodamine B (RhB)] and anionic dyes [methylene orange (MO) and congo red (CR)]. Moreover, the samples can be regenerated and recycled without loss of contaminant removal capacity over successive adsorption and washing cycles.
Cellular dosimetry of [177Lu]Lu-DOTA-[Tyr3]octreotate radionuclide therapy: the impact of modeling assumptions on the correlation with in vitro cytotoxicity
Authors: Tamborino Giulia , De Saint-Hubert Marijke , Struelens Lara , Seoane Dayana C. , Ruigrok Eline A. M. , Aerts An , van Cappellen Wiggert A. , de Jong Marion , Konijnenberg Mark W. , Nonnekens Julie 
Published: February 2020

Abstract
Background
Survival and linear-quadratic model fitting parameters implemented in treatment planning for targeted radionuclide therapy depend on accurate cellular dosimetry. Therefore, we have built a refined cellular dosimetry model for [177Lu]Lu-DOTA-[Tyr3]octreotate (177Lu-DOTATATE) in vitro experiments, accounting for specific cell morphologies and sub-cellular radioactivity distributions.

Methods
Time activity curves were measured and modeled for medium, membrane-bound, and internalized activity fractions over 6 days. Clonogenic survival assays were performed at various added activities (0.1–2.5 MBq/ml). 3D microscopy images (stained for cytoplasm, nucleus, and Golgi) were used as reference for developing polygonal meshes (PM) in 3DsMax to accurately render the cellular and organelle geometry. Absorbed doses to the nucleus per decay (S values) were calculated for 3 cellular morphologies: spheres (MIRDcell), truncated cone-shaped constructive solid geometry (CSG within MCNP6.1), and realistic PM models, using Geant4-10.03. The geometrical set-up of the clonogenic survival assays was modeled, including dynamic changes in proliferation, proximity variations, and cell death. The absorbed dose to the nucleus by the radioactive source cell (self-dose) and surrounding source cells (cross-dose) was calculated applying the MIRD formalism. Finally, the correlation between absorbed dose and survival fraction was fitted using a linear dose-response curve (high α/β or fast sub-lethal damage repair half-life) for different assumptions, related to cellular shape and localization of the internalized fraction of activity.

Results
The cross-dose, depending on cell proximity and colony formation, is a minor (15%) contributor to the total absorbed dose. Cellular volume (inverse exponential trend), shape modeling (up to 65%), and internalized source localization (up to + 149% comparing cytoplasm to Golgi) significantly influence the self-dose to nucleus. The absorbed dose delivered to the nucleus during a clonogenic survival assay is 3-fold higher with MIRDcell compared to the polygonal mesh structures. Our cellular dosimetry model indicates that 177Lu-DOTATATE treatment might be more effective than suggested by average spherical cell dosimetry, predicting a lower absorbed dose for the same cellular survival. Dose-rate effects and heterogeneous dose delivery might account for differences in dose-response compared to x-ray irradiation.

Conclusion
Our results demonstrate that modeling of cellular and organelle geometry is crucial to perform accurate in vitro dosimetry.
The effect of educational intervention on healthcare providers’ knowledge, attitude, & practice towards antimicrobial stewardship program at, National Liver Institute, Egypt
Authors: Tahoon Marwa Ali , Khalil Magdy Mohammed , Hammad Essam , Morad Wesam Saber , awad Samah Mohammed , Ezzat Sameera 
Published: February 2020

Abstract
Background
Inappropriate use of antimicrobials results in rapid emergence of resistance, selection pressure on resistant microorganisms, adverse reactions, and treatment failures. An effective approach to improving antimicrobial use in healthcare settings is a structured antimicrobial management program known as antimicrobial stewardship (AMS). Education and training is considered a backbone element of AMS program .This study aimed to evaluate the impact of educational program on the knowledge, attitude, and practice (KAP) of healthcare providers (HCPs) towards antimicrobial stewardship before and after the educational program at the surgery department and surgical ICU, National Liver Institute hospital (NLI), Egypt.

Results
Among the 69 invited HCPs, 48 attended the educational program sessions with attendance response rate about 70%. Regarding pre-educational KAP score of the respondents’ physicians and pharmacists, 39.3% of them had good knowledge score, 85.7% of them had positive attitude, &and 31.8% of physicians had good practice score; however, 100% of the pharmacists had poor practice. Regarding the respondents’ nurse, the pre-education mean score of knowledge was 13.3/20, attitude 14.8/16, and practice 9.3/14. Following educational program, there was a significant improvement in knowledge, attitude, and practice of the respondents’ health care providers (P < 0.001); on the contrary, there was no significant improvement in the practice of pharmacists (P > 0.05).

Conclusion
The implemented educational program was successful in improving the knowledge, attitude, and practice of HCPs; therefore, continuous efforts are needed to implement more educational programs to increase awareness towards AMS among HCPs.
The structure of mania: An overview of factorial analysis studies
Authors: Martino Diego J. , Valerio Marina P. , Parker Gordon 
Published: February 2020

AbstractBackground.Operational definitions of mania are based on expert consensus rather than empirical data. The aim of this study is to identify the key domains of mania, as well as the relevance of the different signs and symptoms of this clinical construct.Methods.A review of latent factor models studies in manic patients was performed. Before extraction, a harmonization of signs and symptoms of mania and depression was performed in order to reduce the variability between individual studies.Results.We identified 12 studies fulfilling the inclusion criteria and comprising 3039 subjects. Hyperactivity was the clinical item that most likely appeared in the first factor, usually covariating with other core features of mania, such as increased speech, thought disorder, and elevated mood. Depressive–anxious features and irritability–aggressive behavior constituted two other salient dimensions of mania. Altered sleep was frequently an isolated factor, while psychosis appeared related to grandiosity, lack of insight and poor judgment.Conclusions.Our results confirm the multidimensional nature of mania. Hyperactivity, increased speech, and thought disorder appear as core features of the clinical construct. The mood experience could be heterogeneous, depending on the co-occurrence of euphoric (elevated mood) and dysphoric (irritability and depressive mood) emotions of varying intensity. Results are also discussed regarding their relationship with other constitutive elements of bipolar disorder, such as mixed and depressive states.
“Not a Figure in the Past”
Authors: May Chelsie 
Published: February 2020

AbstractThis article uses the racial divisions encouraged by European Zionism in early-state Israel among European and Middle Eastern Jews as a point of departure to explore racialization and gendering among Iraqi Jewish women during the years 1941–51 from a sociopolitical standpoint. Restricting itself to politics given this standpoint, a study of Jewish women’s participation in the illegal Zionist and Communist movements of Iraq reveals that racializations, rather than a single racialization, occurred—a racial reality no other scholarship provides for Iraq’s Jewish community. Because Jewish women participating in these movements contended with patriarchal organizing structures, it is necessary to set apart the racial logics palpable in their articulations. This argument rests on primary sources in the form of three memoirs from the Iraqi Jewish women Tikva Agassi, Shoshana Levy, and Shoshana Almoslino, as well as Zionist women’s letters, a biographical dictionary of Communist participation, and British Foreign Office documents.
Sexual cell cycle initiation is regulated by CDK19 and CYC9 in Tetrahymena thermophila
Authors: Ma Yang , Yan Guanxiong , Han Xiaojie , Zhang Jing , Xiong Jie , Miao Wei 
Published: February 2020

ABSTRACT
To investigate the mechanisms underlying initiation of the sexual cell cycle in eukaryotes, we have focused on cyclins and cyclin-dependent kinases (CDKs) in the well-studied model ciliate, Tetrahymena thermophila. We identified two genes, CDK19 and CYC9, which are highly co-expressed with the mating-associated factors MTA, MTB and HAP2. Both CDK19 and CYC9 were found to be essential for mating in T. thermophila. Subcellular localization experiments suggested that these proteins are located at the oral area, including the conjugation junction area, and that CDK19 or CYC9 knockout prevents mating. We found that CDK19 and CYC9 form a complex, and also identified several additional subunits, which may have regulatory or constitutive functions. RNA sequencing analyses and cytological experiments showed that mating is abnormal in both ΔCDK19 and ΔCYC9, mainly at the entry to the co-stimulation stage. These results indicate that the CDK19–CYC9 complex initiates the sexual cell cycle in T. thermophila.
The Sts1 nuclear import adapter uses a non-canonical bipartite nuclear localization signal and is directly degraded by the proteasome
Authors: Budenholzer Lauren , Breckel Carolyn , Hickey Christopher M. , Hochstrasser Mark 
Published: February 2020

ABSTRACT
The proteasome is an essential regulator of protein homeostasis. In yeast and many mammalian cells, proteasomes strongly concentrate in the nucleus. Sts1 from the yeast Saccharomyces cerevisiae is an essential protein linked to proteasome nuclear localization. Here, we show that Sts1 contains a non-canonical bipartite nuclear localization signal (NLS) important for both nuclear localization of Sts1 itself and the proteasome. Sts1 binds the karyopherin-α import receptor (Srp1) stoichiometrically, and this requires the NLS. The NLS is essential for viability, and over-expressed Sts1 with an inactive NLS interferes with 26S proteasome import. The Sts1–Srp1 complex binds preferentially to fully assembled 26S proteasomes in vitro. Sts1 is itself a rapidly degraded 26S proteasome substrate; notably, this degradation is ubiquitin independent in cells and in vitro and is inhibited by Srp1 binding. Mutants of Sts1 are stabilized, suggesting that its degradation is tightly linked to its role in localizing proteasomes to the nucleus. We propose that Sts1 normally promotes nuclear import of fully assembled proteasomes and is directly degraded by proteasomes without prior ubiquitylation following karyopherin-α release in the nucleus.
ORP3 phosphorylation regulates phosphatidylinositol 4-phosphate and Ca2+ dynamics at plasma membrane–ER contact sites
Authors: Gulyás Gergő , Sohn Mira , Kim Yeun Ju , Várnai Péter , Balla Tamas 
Published: February 2020

ABSTRACT
Oxysterol-binding protein (OSBP)-related proteins (ORPs) mediate non-vesicular lipid transfer between intracellular membranes. Phosphoinositide (PI) gradients play important roles in the ability of OSBP and some ORPs to transfer cholesterol and phosphatidylserine between the endoplasmic reticulum (ER) and other organelle membranes. Here, we show that plasma membrane (PM) association of ORP3 (also known as OSBPL3), a poorly characterized ORP family member, is triggered by protein kinase C (PKC) activation, especially when combined with Ca2+ increases, and is determined by both PI(4,5)P2 and PI4P. After activation, ORP3 efficiently extracts PI4P and to a lesser extent phosphatidic acid from the PM, and slightly increases PM cholesterol levels. Full activation of ORP3 resulted in decreased PM PI4P levels and inhibited Ca2+ entry via the store-operated Ca2+ entry pathway. The C-terminal region of ORP3 that follows the strictly defined lipid transfer domain was found to be critical for the proper localization and function of the protein.
Pumping the brakes on RAS – negative regulators and death effectors of RAS
Authors: Harrell Stewart Desmond R. , Clark Geoffrey J. 
Published: February 2020

ABSTRACT
Mutations that activate the RAS oncoproteins are common in cancer. However, aberrant upregulation of RAS activity often occurs in the absence of activating mutations in the RAS genes due to defects in RAS regulators. It is now clear that loss of function of Ras GTPase-activating proteins (RasGAPs) is common in tumors, and germline mutations in certain RasGAP genes are responsible for some clinical syndromes. Although regulation of RAS is central to their activity, RasGAPs exhibit great diversity in their binding partners and therefore affect signaling by multiple mechanisms that are independent of RAS. The RASSF family of tumor suppressors are essential to RAS-induced apoptosis and senescence, and constitute a barrier to RAS-mediated transformation. Suppression of RASSF protein expression can also promote the development of excessive RAS signaling by uncoupling RAS from growth inhibitory pathways. Here, we will examine how these effectors of RAS contribute to tumor suppression, through both RAS-dependent and RAS-independent mechanisms.
Dynamic changes in cytoskeleton proteins of olfactory ensheathing cells induced by radiofrequency electromagnetic fields
Authors: Grasso Rosaria , Pellitteri Rosalia , Caravella Santi A. , Musumeci Francesco , Raciti Giuseppina , Scordino Agata , Sposito Giovanni , Triglia Antonio , Campisi Agata 
Published: February 2020

Several evidences have suggested the ability of radio frequency electromagnetic fields to influence biological systems, even if the action mechanisms are not well understood. Only few data have reported about the effect of radio frequency electromagnetic fields on self-renewal of neural progenitor cells. A particular glial type, which shows characteristics of stem cells, are Olfactory Ensheathing Cells (OECs). Herein, we assessed the non-thermal effects induced on Olfactory Ensheathing Cells through radio frequency electromagnetic fields changing the envelope of the electromagnetic wave. Primary OEC cultures were exposed to continuous or amplitude modulated 900 MHz electromagnetic fields, in far field condition and at different exposition times (10, 15, 20 min). The expression of Olfactory Ensheathing Cells markers (S-100 and Nestin), cytoskeletal proteins (GFAP and Vimentin), apoptotic pathway activation by Caspase-3 cleavage and cell viability were evaluated.
Our results highlight that 20 min of exposure to continuous or amplitude modulated 900 MHz electromagnetic fields induced a different and significant decrease in cell viability. In addition, according to the electromagnetic fields waveform, diverse dynamic changes in the expression of the analysed markers in Olfactory Ensheathing Cells and activation of apoptotic pathway were observed. The data suggest that radio frequency electromagnetic fields might play different and important role in the self-renewal of OEC stem cells, which are involved in nervous system repair.
Dynamism of Supramolecular DNA/RNA Nanoarchitectonics: From Interlocked Structures to Molecular Machines
Authors: Liang Xingguo , Li Lin , Tang Jiaxuan , Komiyama Makoto , Ariga Katsuhiko 
Published: February 2020

AbstractIn order to fabricate highly sophisticated nanostructures, various research fields must be fused into one unified concept. In recently emerging nanoarchitectonics, nanotechnology is combined with materials science, supramolecular self-assembly, and biological science to construct functional materials from nanoscale units. This review article focuses on dynamic features of nanoarchitectonics. In terms of this concept, molecular machines as one of the forefront research targets are produced from DNA and RNA. Fundamental designs of DNA/RNA molecular machines are first described with an emphasis on the significant roles of interlocked structures such as rings, catenanes, rotaxanes, and connectors of these parts. In the latter part, DNA/RNA molecular machines including shuttles, molecular transporters, walkers, nano pumps, robots, amplifiers, and logic gates are presented, together with related functions such as sensitive sensing and catalysis regulation. The exemplified strategy should be universally useful to fabricate various functional materials which fulfill social demands at the present as well as in the future.
The mystery of the Votic inessive
Authors: Markus Elena , Rozhanskiy Fedor 
Published: February 2020

This paper analyzes the morphophonological structure of the inessive singular forms in Liivtšülä-Luuditsa Votic. There are no fluent speakers of this variety; the research is based on the materials recorded in 2003–2016. The inessive singular forms demonstrate variation of the weak and strong grade stems, which is very untypical for a case form. In all other morphological cases, the distribution of weak and strong stems is very stable. The variation in the inessive stem is observed both in the examples from published sources on the Votic language, and in our field materials. The acoustic research has confirmed the variation. Additionally, in the strong grade stem we find a shorter geminate stop or affricate compared to other strong grade forms (in the paper, the inessive singular is compared to the inessive plural and partitive singular). We consider several hypotheses that could explain the variation and the shorter geminate. The conducted experiments do not confirm the role of the word structure as the primary factor defining the geminate length. We suggest that both the variation of stems and the shorter geminate might result from language contact. In the neighbouring Ingrian varieties, the inessive is built from the weak grade stem. Since all the last fluent speakers of Votic knew Ingrian to some extent, the Votic and Ingrian patterns might have mixed. It is probable that originally the variation was triggered by some other factors, and the increasing role of the language contact turned it into a dominant factor in the course of the 20th century.
Kokkuvõte. Elena Markus ja Fedor Rozhanskiy: Vadja inessiivi mõistatus. Artiklis analüüsitakse ainsuse inessiivi morfofonoloogilist struktuuri Liivtšülä- Luuditsa vadja keeles. Selle keele soravaid kõnelejaid enam pole; uurimus põhineb 2003–2016 lindistatud materjalidel. Ainsuse inessiivi vormid näitavad nõrga- ja tugevaastmeliste tüvede varieerumist, mis on ühe käändevormi kohta väga ebatüüpiline. Kõikide teiste morfoloogiliste käänete puhul on nõrkade ja tugevate tüvede jaotus korrapärane. Inessiivi tüve varieerumist vaadeldakse nii varem avaldatud vadja keele allikates kui ka meie välitööde materjalides. Akustiline analüüs kinnitab tüvede varieerumist. Lisaks esineb tugevas astmes inessiivis lühem geminaatkonsonant või afrikaat kui teistes tugeva astme vormides (artiklis võrreldakse ainsuse inessiivi mitmuse inessiivi ja ainsuse partitiiviga). Me esitame mõned hüpoteesid, mis võiksid sellist varieerumist ja lühemat geminaati selgitada. Tehtud eksperimendid ei kinnita sõnastruktuuri rolli esmase faktorina geminaadi pikkuse defineerimisel. Me arvame, et nii tüvede varieerumine kui ka lühike geminaat võivad olla tingitud keelekontaktist. Naabruses olevates isuri murretes moodustatakse inessiiv nõrgaastmelisest tüvest. Kuna kõik viimased head vadja keele kõnelejad oskasid mingil määral isuri keelt, võisid vadja ja isuri keel olla segunenud. On tõenäoline, et algselt oli varieerumine tingitud teistest faktoritest, kuid keelekontakti suurenenud roll kujunes 20. sajandi jooksul domineerivaks.
Features of immune status in different states of uric acid metabolism in female rats
Authors: Gozhenko Anatoliy , Smagliy Vadym , Korda Inna , Badiuk Nataliya , Zukow Walery , Popovych Igor 
Published: February 2020

Background. Previously, we found a wide range of uric acid metabolism parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy female rats analyzed. It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%. The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism. Material and Methods. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined. In the blood, the parameters of immunity were determined. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon’s entropy was calculated. Results. Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functional-metabolic patterns, two of which are quasilinear (enhancing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. Conclusion. Endogenous uric acid exerts a modulatory immunotropic effect in healthy female rats.
The determinants of footballers' effectiveness in a German club
Authors: Kalinowski Paweł , Kalinowski Sebastian , Jerszyński Damian , Nowakowska Monika , Konarski Jan , Pietranis Dariusz 
Published: February 2020

The assessment of footballers effectiveness is the key element of control at every step of their training, regardless of on what level they are. What seems essential is the prediction of footballers' actions, the height of which is achieved in the time directly preceding taking part in competition. Therefore, twenty footballers took part in the study which involved efficiency tests concerning maximum speed in 5 meters and 20 meters races as well as endurance measured by Beep Test. The tests were conducted at the end of lead time. Additionally, the effectiveness of action was measured in the initial perdion. No significant correlations were found between the tested variables – speed tests and effectiveness of game. A significant relationship was found only between the endurance measured by Beep Test.Summing up, the only factor significantly correlated to the effectiveness of footballers' action was endurance and it can be therefore seen as crucial in terms of motor preparation, at the tested sport level.