bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in Water and Sewage Management after Communism: example of the Oder River Basin (Central Europe)
Authors: Marszelewski Włodzimierz , Piasecki Adam 
Published: April 2020

AbstractThis paper presents changes in water and sewage management in the cross-border Oder River basin in the period since the post-communist political and economic system transformation, including the period after Poland’s accession to the European Union. The Oder River basin, with an area of 124,000 km2, is the second largest basin in the Baltic Sea Basin, and therefore requires particular protection. It was emphasised that in the years 1989–2017, water withdrawal for production purposes considerably decreased (by 42%), as well as water withdrawal for exploitation of the water supply system (by 33%). The amount of sewage discharged to rivers was also reduced (by approximately 50%), and treatment technologies considerably improved. Changes in water and sewage management were presented in spatial form, i.e. by hydrographic regions of the Oder River basin. Particular attention was paid to changes in sewage management in cities. They involved among others the liquidation of mechanical treatment plants and a considerable increase in the number of cities with treatment plants with increased nutrient removal. The analysis of the effect of the changes in water and sewage management on the quality of the Oder River and Baltic Sea was also performed, and the rate of decrease in loads of contaminants most harmful to water ecosystems was determined. The role of European Union funds and national funds in the implementation of investments in the scope of water management was emphasised. Finally, attention was drawn to the need to intensify works for protecting waters in agricultural areas, which currently constitute the primary threat to their quality. Several top-priority tasks were also specified that should be implemented in the near future for the purpose of obtaining a good ecological state of waters in the Oder River basin pursuant to the Water Framework Directive.
Adherence to nine-month isoniazid for latent tuberculosis infection in healthcare workers: a prospective study in a tertiary hospital
Authors: Chung Sung Jun , Lee Hyun , Koo Gun Woo , Min Ji-Hee , Yeo Yoomi , Park Dong Won , Park Tai Sun , Moon Ji-Yong , Kim Sang-Heon , Kim Tae Hyung , Sohn Jang Won , Yoon Ho Joo 
Published: April 2020

AbstractPoor adherence to medication can lead to treatment failure in healthcare workers (HWCs) with latent tuberculosis infection (LTBI) who are at high risk of developing active tuberculosis. However, the factors associated with non-completion of nine-month LTBI treatment with isoniazid (9 H) have not been well studied. We investigated the completion rate and factors affecting adherence to LTBI treatment with 9 H among HCWs. A prospective cohort study of 114 HCWs who were diagnosed with LTBI by QuantiFERON-TB Gold In-Tube tests were performed in a single university hospital between June 2016 and December 2017. All patients received the 9 H LTBI treatment. At each visit, treatment adherence and development of adverse reactions to isoniazid were evaluated via a standard questionnaire. To evaluate the impact of the severity of hepatotoxicity on non-completion of LTBI treatment, we classified hepatotoxicity into two groups: severe hepatotoxicity was defined as alanine aminotransferase >3.0 times the upper normal limit (UNL) with symptoms or  = 5.0 times the UNL. Mild hepatotoxicity was defined as alanine aminotransferase>UNL, but not meet the definition of severe hepatotoxicity. Overall, 71 HCWs (62.3%) completed LTBI treatment with 9 H while 43 HCWs (37.7%) discontinued their treatment. Most discontinuation (81.4%, 35/43) occurred during the first three months of treatment. There were no significant differences in age, sex, occupation, or comorbidities between the HCWs who completed and those who discontinued LTBI treatment. However, HCWs who discontinued LTBI treatment had more hepatotoxicity than those who completed treatment (44.2% vs. 11.3%, P < 0.001). Cox proportional hazard analysis revealed that hepatotoxicity is the only factor significantly associated with discontinuation of 9 H LTBI treatment (unadjusted HR = 2.89, 95% CI = 1.62–5.46). In multivariable analysis, not only severe hepatotoxicity (adjusted HR = 7.99, 95% CI = 3.05–20.94) but also mild hepatotoxicity was significantly associated with discontinuation of LTBI treatment (adjusted HR = 2.34, 95% CI = 1.05–5.21). The completion rate of 9 H LTBI treatment was 62.3% among HCWs. While age, sex, occupation, and pretreatment comorbidities were not associated with treatment completion, isoniazid-induced hepatotoxicity significantly affected adherence.
Trace elements and C and N isotope composition in two mushroom species from a mine-spill contaminated site
Authors: Gil-Martínez Marta , Navarro-Fernández Carmen M. , Murillo José M. , Domínguez María T. , Marañón Teodoro 
Published: April 2020

AbstractFungi play a key role in the functioning of soil in terrestrial ecosystems, and in particular in the remediation of degraded soils. The contribution of fungi to carbon and nutrient cycles, along with their capability to mobilise soil trace elements, is well-known. However, the importance of life history strategy for these functions has not yet been thoroughly studied. This study explored the soil-fungi relationship of two wild edible fungi, the ectomycorrhizalLaccaria laccataand the saprotrophVolvopluteus gloiocephalus. Fruiting bodies and surrounding soils in a mine-spill contaminated area were analysed. Isotope analyses revealedLaccaria laccatafruiting bodies were15N-enriched when compared toVolvopluteus gloiocephalus, likely due to the transfer of15N-depleted compounds to their host plant. Moreover,Laccaria laccatafruiting bodies δ13C values were closer to host plant values than surrounding soil, whileVolvopluteus gloiocephalusmatched the δ13C composition to that of the soil. Fungal species presented high bioaccumulation and concentrations of Cd and Cu in their fruiting bodies. Human consumption of these fruiting bodies may represent a toxicological risk due to their elevated Cd concentrations.
Spatiotemporal heterogeneity of social contact patterns related to infectious diseases in the Guangdong Province, China
Authors: Huang Yulin , Cai Xiaoshuang , Zhang Bing , Zhu Guanghu , Liu Tao , Guo Pi , Xiao Jianpeng , Li Xing , Zeng Weilin , Hu Jianxiong , Ma Wenjun 
Published: April 2020

AbstractThe social contact patterns associated with the infectious disease transmitted by airborne droplets or close contact follow specific rules. Understanding these processes can improve the accuracy of disease transmission models, permitting their integration into model simulations. In this study, we performed a large-scale population-based survey to collect social contact patterns in three cities on the Pearl River Delta of China in winter and summer. A total of 5,818 participants were face-to-face interviewed and 35,542 contacts were recorded. The average number of contacts per person each day was 16.7 considering supplementary professional contacts (SPCs). Contacts that occurred on a daily basis, lasted more than 4 hours, and took place in households were more likely to involve physical contact. The seasonal characteristics of social contact were heterogeneous, such that contact in the winter was more likely to involve physical contact compared to summer months. The spatial characteristics of the contacts were similar. Social mixing patterns differed according to age, but all ages maintained regular contact with their peers. Taken together, these findings describe the spatiotemporal distribution of social contact patterns relevant to infections in the Guangdong Province of China. This information provides important parameters for mathematical models of infectious diseases.
Nanodiamond phantoms mimicking human liver: perspective to calibration of T1 relaxation time in magnetic resonance imaging
Authors: Sękowska Anna , Majchrowicz Daria , Sabisz Agnieszka , Ficek Mateusz , Bułło-Piontecka Barbara , Kosowska Monika , Jing Lina , Bogdanowicz Robert , Szczerska Małgorzata 
Published: April 2020

AbstractPhantoms of biological tissues are materials that mimic the properties of real tissues. This study shows the development of phantoms with nanodiamond particles for calibration of T1 relaxation time in magnetic resonance imaging. Magnetic resonance imaging (MRI) is a commonly used and non-invasive method of detecting pathological changes inside the human body. Nevertheless, before a new MRI device is approved for use, it is necessary to calibrate it properly and to check its technical parameters. In this article, we present phantoms of tissue with diamond nanoparticles dedicated to magnetic resonance calibration. The method of producing phantoms has been described. As a result of our research, we obtained phantoms that were characterized by the relaxation time T1 the same as the relaxation time of the human tissue T1 = 810.5 ms. Furthermore, the use of diamond nanoparticles in phantoms allowed us to tune the T1 value of the phantoms which open the way to elaborated phantoms of other tissues in the future.
Characterizing pollution and source identification of heavy metals in soils using geochemical baseline and PMF approach
Authors: Jiang Hui-Hao , Cai Li-Mei , Wen Han-Hui , Luo Jie 
Published: April 2020

AbstractIt is necessary to establish local geochemical baseline concentrations (GBCs) due to the lack or the inapplicability of regional background values in the study area. The establishment of GBCs of heavy metal (HM) in soil helps in making the accurate assessment of pollution, and then provides a basis for pollution control. Based on this, a case study was undertaken to study the GBCs of the Jiedong District, Guangdong Province, China. In this research, cumulative frequency distribution curves were utilized to determine the local GBCs in the subsoils. The determined GBCs of Cr, Hg, As, Pb, Ni, Cd, Cu, Zn, Co and V were 39.91, 0.072, 11.48, 47.62, 12.70, 0.17, 14.22, 64.54, 6.31, and 68.14 mg/kg, respectively. The average concentrations of Hg, As, Pb, Cd, Cu and Zn in the topsoils exceeded the corresponding baseline concentrations. In particular, the contents of Cd and Hg were 1.53 and 2.22 times higher than GBCs. According to this baseline criterion, enrichment factor (EF), pollution load index (PLI) and ecological risk index (RI) were applied to assessing HM pollution. EF and PLI suggested that most areas were under moderate contamination, while Hg and Cd pollution was more serious. And the RI values presented that the potential ecological risks were low in most parts of the study area. The possible origins of HMs were identified by combining positive matrix factorization with EF and geostatistics. Comprehensive analysis indicated that Hg and Cd were related to industrial activities, such as textile and garment processing, plastic and rubber production and metal manufacturing. Arsenic and part of Cu mainly came from agricultural activities, namely the use of pesticides, fertilizers and livestock manures. Lead and Zn were mainly attributed to traffic emissions. Chromium, Ni, V, Co, and part of Cu were originated from natural source controlled by parent materials. The corresponding contributions of these sources were 20.61%, 24.20%, 19.22% and 35.97%, respectively. This work provides information to prevent and control the soil HM pollution by proposing the efficient management of anthropogenic sources.
Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer
Authors: Capellero S. , Erriquez J. , Melano C. , Mesiano G. , Genta S. , Pisacane A. , Mittica G. , Ghisoni E. , Olivero M. , Di Renzo M. F. , Aglietta M. , Sangiolo D. , Valabrega G. 
Published: April 2020

AbstractDespite improvements in surgery and medical treatments, epithelial ovarian cancer (EOC) remains the most lethal gynaecological malignancy. Aim of this study is to investigate the preclinical immunotherapy activity of cytokine-induced killer lymphocytes (CIK) against epithelial ovarian cancers, focusing on platinum-resistant settings. We generated CIKex vivostarting from human peripheral blood samples (PBMCs) collected from EOC patients. Their antitumor activity was testedin vitroandin vivoagainst platinum-resistant patient-derived ovarian cancer cells (pdOVCs) and a Patient Derived Xenograft (PDX), respectively. CIK were efficiently generated (48 fold medianex vivoexpansion) from EOC patients; pdOVCs lines (n = 9) were successfully generated from metastatic ascites; the expression of CIK target molecules by pdOVC confirmed pre and post treatmentin vitrowith carboplatin. The results indicate that patient-derived CIK effectively killed autologous pdOVCsin vitro. Such intense activity was maintained against a subset of pdOVC that survivedin vitrotreatment with carboplatin. Moreover, CIK antitumor activity and tumor homing was confirmedin vivowithin an EOC PDX model. Our preliminary data suggest that CIK are active in platinum resistant ovarian cancer models and should be therefore further investigated as a new therapeutic option in this extremely challenging setting.
The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission
Authors: Kim Ji-hyung , Ki Yoonhee , Lee Hoyeon , Hur Moon Seong , Baik Bukyung , Hur Jin-Hoe , Nam Dougu , Lim Chunghun 
Published: April 2020

AbstractGenes and neural circuits coordinately regulate animal sleep. However, it remains elusive how these endogenous factors shape sleep upon environmental changes. Here, we demonstrate that Shaker (Sh)-expressing GABAergic neurons projecting onto dorsal fan-shaped body (dFSB) regulate temperature-adaptive sleep behaviors in Drosophila. Loss of Sh function suppressed sleep at low temperature whereas light and high temperature cooperatively gated Sh effects on sleep. Sh depletion in GABAergic neurons partially phenocopied Sh mutants. Furthermore, the ionotropic GABA receptor, Resistant to dieldrin (Rdl), in dFSB neurons acted downstream of Sh and antagonized its sleep-promoting effects. In fact, Rdl inhibited the intracellular cAMP signaling of constitutively active dopaminergic synapses onto dFSB at low temperature. High temperature silenced GABAergic synapses onto dFSB, thereby potentiating the wake-promoting dopamine transmission. We propose that temperature-dependent switching between these two synaptic transmission modalities may adaptively tune the neural property of dFSB neurons to temperature shifts and reorganize sleep architecture for animal fitness.
Commentary on: The potency of lncRNA MALAT1/miR-155 in altering asthmatic Th1/Th2 balance by modulation of CTLA4
Authors: Foronjy Robert 
Published: April 2020

Abstract
Asthma is a common, allergic respiratory disorder affecting over 350 million people worldwide. One of the key features of asthma is skewing of CD4+ cells toward Th2 responses. This promotes the production of cytokines like IL-4 that induce IgE production resulting in the hypersecretion of mucus and airway smooth muscle contraction. Understanding the factors that favor Th2 expansion in asthma would provide important insights into the underlying pathogenesis of this disorder. Asthma research has focused on signaling pathways that control the transcription of key asthma-related genes. However, increasing evidence shows that post-transcriptional factors also determine CD4+ cell fate and the enhancement of allergic airway responses. A recent paper published by Liang et al. (Bioscience Reports (2020) 40, https://doi.org/10.1042/BSR20190397) highlights a novel role for the long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in Th2 development in asthma. MALAT1 modulates several biological processes including alternative splicing, epigenetic modification and gene expression. It is one of the most highly expressed lncRNAs in normal tissues and MALAT1 levels correlate with poor clinical outcomes in cancer. The mechanisms of action of MALAT1 in tumor progression and metastasis remain unclear and even less is known about its effects in inflammatory disease states like asthma. The work of Liang et al. demonstrates heightened MALAT1 expression in asthma and further shows that this lncRNA targets miR-155 to promote Th2 differentiation in this disease. This insight sets the stage for future studies to examine how MALAT1 manipulation could deter allergic immune responses in asthmatic airways.
Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p
Authors: Pang Wenguang , Huang Fengliu , Zhang Xin , Ye Min , Huang Yanming , Huang Xiufang , Pang Jingzhuo , Cai Chengjie , Wang Zheng 
Published: April 2020

Abstract
Objective: Non-small cell lung cancer (NSCLC) continues to top the list of cancer mortalities worldwide. Early diagnosis and therapeutic interventions targeting NSCLC is becoming the world’s significant challenge. Circular RNAs (circRNAs) are emerging as a group of potential cancer biomarkers.
Materials and methods: Quantitative real-time PCR (qRT-PCR) was employed to examine the expression of circ_0072309 in NSCLC tissues and cell lines. Cell counting kit 8 (CCK-8), wound healing and Transwell assays were used to analyze cell proliferation, migration and invasion in A549 and H1299 cells. The relationship between circ_0072309 and miR-580-3 was analyzed by Luciferase reporter and RNA pull down assays.
Results: We screened circ_0072309 from Gene Expression Omnibus and found that circ_0072309 was lowly expressed in NSCLC tissues and cell lines. The transfection of circ_0072309-overexpressing vector significantly suppressed the cell proliferation, migration and invasion in A549 and H1299 cells. We predicted that miR-580-3p is a target of circ_0072309 by using publicly available bioinformatic algorithms Circinteractome tool and confirmed that circ_0072309 directly bound to miR-580-3p. Furthermore, the addition of miR-580-3p mitigated the blockage of cell proliferation, migration and invasion induced by circ_0072309.
Conclusions: These data showed that circ_0072309 inhibits the progression of NSCLC progression via blocking the expression of miR-580-3p. These findings revealed the anti-tumor role of circ_0072309 during the development of NSCLC and provided a novel diagnostic biomarker and potential therapy for NSCLC.
Tripartite motif containing 35 contributes to the proliferation, migration, and invasion of lung cancer cellsin vitroandin vivo
Authors: Zhang Jingtao , Xu Zihao , Yu Boyao , Xu Jiatang , Yu Bentong 
Published: April 2020

AbstractThe tripartite motif (TRIM) family is a family of proteins with highly conserved domains. Previous researches have suggested that the members of TRIM family proteins played a crucial role in cancer development and progression. Our study explored the relationship between TRIM35 and non-small cell lung cancer (NSCLC). The study showed that the expression of TRIM35 was increased in NSCLC samples, and patients with high expression of TRIM35 had a poor clinical prognosis. Overexpression of TRIM35 in NSCLC cell line H460 promoted cell proliferation, migration, and invasion, knockdown of TRIM35 produced an opposite result in A549 and H1299 cell lines. In vivo study further confirmed that overexpression of TRIM35 promoted tumor formation. The RNA-seq analysis suggested that TRIM35 might promote lung cancer proliferation, migration, and invasion by regulating cancer-associated functions and signaling pathways. Hence, we identified TRIM35 played a significant role in tumoral growth and was a potential diagnosis and prognosis target for lung cancer.
Parallax in microlensing toward the Magellanic Clouds: Effect on detection efficiency and detectability
Authors: Blaineau T. , Moniez M. 
Published: April 2020

Aims. We study the effect of the parallax on the search for microlensing events on very long timescales towards the Magellanic Clouds caused by dark massive compact objects within the past MACHO and EROS, the on-going MOA and OGLE, and the future LSST surveys. We quantify what neglecting this effect means in the classical event selection process and also quantify the parallax detectability without the help of follow-up observations.
Methods. We defined the distance between true events affected by parallax and the closest events without parallax. This distance was used to estimate the probability of missing the preselection of events because of parallax for any survey characterised by its time sampling and photometric performance. We also defined another distance to quantify the detectability of the parallax effect in order to trigger complementary observations.
Results. We find that the preselection of year-long timescale events is marginally affected by parallax for all surveys if the criteria are reasonably tight. We also show that the parallax should be detectable in most of the events found by the LSST survey without follow-up observations.
Isolation, Characterization and Targeted Metabolic Evaluation of Endophytic Fungi Harbored in 14 Seed-Derived Hypericum Species
Authors: Henzelyová Jana , Antalová Michaela , Nigutová Katarína , Logoida Mariia , Schreiberová Andrea , Kusari Souvik , Čellárová Eva 
Published: April 2020

AbstractMedicinal plants of the genus Hypericum are rich sources of bioactive naphthodianthrones, which are unique in the plant kingdom, but quite common in fungal endophytes. Cultivable endophytic fungi were isolated from 14 different Hypericum spp. originating from seeds grown under in vitro conditions and further acclimated to outdoor conditions. Among 37 fungal isolates yielded from the aerial and underground plant organs, 25 were identified at the species level by the fungal barcode marker internal transcribed spacer rDNA and protein-coding gene region of tef1α. Ten of them were isolated from Hypericum spp. for the first time. The axenic cultures of the isolated endophytes were screened for the production of extracellular enzymes, as well as bioactive naphthodianthrones and their putative precursors by Bornträgerʼs test and HPLC-HRMS. Traces of naphthodianthrones and their intermediates, emodin, emodin anthrone, skyrin, or pseudohypericin, were detected in the fungal mycelia of Acremonium sclerotigenum and Plectosphaerella cucumerina isolated from Hypericum perforatum and Hypericum maculatum, respectively. Traces of emodin, hypericin, and pseudohypericin were released in the broth by Scedosporium apiospermum, P. cucumerina, and Fusarium oxysporum during submerged fermentation. These endophytes were isolated from several hypericin-producing Hypericum spp. Taken together, our results reveal the biosynthetic potential of cultivable endophytic fungi harbored in Hypericum plants as well as evidence of the existence of remarkable plant-endophyte relationships in selected non-native ecological niches. A possible role of the extracellular enzymes in plant secondary metabolism is discussed.