bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary cilia control cell alignment and patterning in bone development via ceramide-PKCζ-β-catenin signaling
Authors: Lim Jormay , Li Xinhua , Yuan Xue , Yang Shuting , Han Lin , Yang Shuying 
Published: January 2020

AbstractIntraflagellar transport (IFT) proteins are essential for cilia assembly and function. IFT protein mutations lead to ciliopathies, which manifest as variable skeletal abnormalities. However, how IFT proteins regulate cell alignment during bone development is unknown. Here, we show that the deletion of IFT20 in osteoblast lineage using Osterix-Cre and inducible type I Collagen-CreERT cause a compromised cell alignment and a reduced bone mass. This finding was validated by the disorganized collagen fibrils and decreased bone strength and stiffness in IFT20-deficient femurs. IFT20 maintains cilia and cell alignment in osteoblasts, as the concentric organization of three-dimensional spheroids was disrupted by IFT20 deletion. Mechanistically, IFT20 interacts with the ceramide-PKCζ complex to promote PKCζ phosphorylation in cilia and induce the apical localization of β-catenin in osteoblasts, both of which were disrupted in the absence of IFT20. These results reveal that IFT20 regulates polarity and cell alignment via ceramide-pPKCζ-β-catenin signaling during bone development.
Pressure and temperature induced red-shift of the sodium D-line during HMX deflagration
Authors: Morley Olivia J. , Williamson David M. 
Published: January 2020

AbstractThe sodium D-line is often present in optical spectra of combustion due to its high prevalence and emissivity. Collision theory predicts the spectral peak to have a red-shift dependent on pressure,P, and temperature,T. Here we show that the conditions reached during deflagration of octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) permit the red-shift of the sodium D-line to be calibrated to 1.5 GPa. Deflagration at these pressures is achieved using a split Hopkinson pressure bar apparatus, with temperatures ofcirca2900 K from the greybody continuum away from spectral features. Lower deflagration pressures, of 0.5 to 0.9 GPa, are achieved in a fallhammer test, with temperatures ofcirca4000 K. The red-shift exhibits the predictedPT−0.7dependence with a constant of proportionality of (950 ± 30) GPa-1 · K0.7 · nm. Using the serendipitous presence of sodium, this optical technique allows for fast measurements of both pressure and temperature from the same light source in one measurement.
LncRNA MCM3AP-AS1 inhibits cell proliferation in cervical squamous cell carcinoma by down-regulating miRNA-93
Authors: Lan Liu , Liang Zhishan , Zhao Yingxi , Mo Yuzhen 
Published: January 2020

Abstract
Background: MCM3AP antisense RNA 1 (MCM3AP-AS1) is characterized as an oncogenic lncRNA in hepatocellular carcinoma and glioblastoma. We analyzed TCGA dataset and observed the down-regulation of MCM3AP-AS1 in cervical squamous cell carcinoma (CSCC). The present study was therefore performed to investigate the role of MCM3AP-AS1 in CSCC.
Methods: A total of 64 female patients with CSCC (38–68 years old; mean age: 53.1 ± 6.5 years old) were enrolled in the present study. RT-qPCR was performed to evaluate gene expression. Methylation specific PCR (MSP) was performed to assess the methylation of miR-93 gene after the overexpression and silencing of MCM3AP-AS1. Cell transfections were performed to investigate the interactions between MCM3AP-AS1 and miR-93. Cell proliferation was assessed by CCK-8 assay.
Results: The results showed that MCM3AP-AS1 was down-regulated in CSCC and predicted poor survival. The expression levels of MCM3AP-AS1 were inversely correlated with the expression levels of miR-93. Overexpression of MCM3AP-AS1 led to down-regulation of miR-93, while silencing of MCM3AP-AS1 played an opposite role in CSCC cells. Methylation-specific PCR revealed that MCM3AP-AS1 could positively regulate the methylation of miR-93 gene. Cell proliferation analysis showed that overexpression of MCM3AP-AS1 led to reduced proliferation rate of CSCC cells. Silencing of MCM3AP-AS1 played an opposite role and overexpression of miR-93 reduced the effects of overexpressing MCM3AP-AS1.
Conclusions: Therefore, MCM3AP-AS1 may inhibit cell proliferation in CSCC by down-regulating miRNA-93.
The Use of Written Naming and Repetition to Treat Naming Deficits in Aphasia
Authors: Hashimoto Naomi 
Published: January 2020

Purpose
The purpose of the study was to examine the use of a combined written naming + repetition (WN + REP) approach and an REP-only approach to treat naming deficits in a group of individuals with aphasia.

Method
Six individuals participated in the study. A multiple-baseline design across behaviors was used. A WN + REP approach, an REP approach, and an untreated condition were presented. Three 10-item treatment lists and one 10-item untreated list were created. Each 10-item treatment list was evenly divided so that 5 items were treated using the WN + REP approach and 5 items were treated using the REP approach.

Results
Visual analyses of treatment data revealed improvements in 4 of the 6 participants. Meaningful treatment effect sizes were obtained for 5 of the 6 participants in at least 1 of the approaches across the treatment lists. Slightly more participants obtained meaningful effect sizes in the WN + REP approach than in the REP approach. Use of writing as a strategy to help with naming the pictures verbally increased over the duration of treatment in 5 of the 6 participants.

Conclusions
The findings revealed that both approaches improved spoken naming accuracy in aphasia. Although the WN + REP approach resulted in more meaningful effect sizes than the REP approach, there was no overwhelming advantage in using 1 or the other approach. However, the WN + REP approach promoted writing as a functional strategy that could be used when naming the picture verbally was not successful.
Neuropsychologische Langzeitfolgen und Teilhabestörungen nach aneurysmatischer Subarachnoidalblutung (aSAB)
Authors: Rollnik Jens D. , Adner Anna 
Published: January 2020

ZusammenfassungDas vorliegende Review beschäftigt sich mit kognitiven Störungen und Teilhabebeeinträchtigungen nach aneurysmatischen Subarachnoidalblutungen (aSAB). Diese können auch ohne den Nachweis einer substanziellen Hirnschädigung in der neuropsychologisch zu erwartenden Region auftreten. Als Ursache für diese Diskrepanz gelten neuroinflammatorische Prozesse und oxidativer Stress. Am häufigsten sind das verbale Gedächtnis, visuell-räumliche Fähigkeiten, das Gedächtnis für visuelle Inhalte, Aufmerksamkeit, Arbeitsgedächtnis, Exekutivfunktionen (Planung, Kontrolle, Problemlösung, Aufmerksamkeit, Entscheidungsfindung), die allgemeine kognitive Verarbeitungsgeschwindigkeit und die sprachliche Domäne betroffen. Es zeigt sich, dass das Vorhandensein kognitiver Beeinträchtigungen einen wesentlichen Risikofaktor darstellt, nicht mehr beruflich reintegriert werden zu können. Zusätzlich zu den kognitiven Symptomen treten nach einer aSAB auch gehäuft psychiatrische Symptome wie Angst, Depression und Fatigue auf. Diese wirken sich zusätzlich zu den körperlichen und kognitiven Beeinträchtigungen negativ auf die Teilhabe am beruflichen und sozialen Leben aus, was wiederum zu einer schlechteren Lebensqualität beiträgt.
Recent Applications in the Use of Sulfoxides as Chiral Auxiliaries for the Asymmetric Synthesis of Natural and Biologically Active Products
Authors: Salom-Roig Xavier , Bauder Claude 
Published: January 2020

The contribution of chiral sulfoxides as versatile auxiliaries in the field of organic chemistry has shown a prevalent interest in the asymmetric synthesis of natural products during the last 45 years. In this short review, we report the recent applications of these chiral auxiliaries to the synthesis of natural and biological active products highlighted from 2010 to 2019. We hope to allow the reader to have an overview of the potential of sulfoxide chemistry in the field of enantio­selective synthesis.1	Introduction2	Diastereoselective Additions to Ketones2.1	Reduction of β-Keto Sulfoxides2.2	Reduction of β-Keto Sulfoxides Followed by Bromohydrin Forma	tion3	Synthesis of an α-Amino α′-Sulfinyl Ketone Followed by Diastere	oselective Reduction of the β-Keto Sulfoxide4	Diastereoselective Addition of Carbanionic Chiral Sulfoxides4.1	Addition to an Aldehyde4.1.1	Aldol Reactions4.1.2	Reformatsky-Type Reactions4.2	Additions to Chiral Sulfinimines5	Diastereoselective Cyclization Reactions Directed by a Chiral Sulf	oxide5.1	1,4-Radical Additions5.2	Intramolecular Conjugate Additions5.3	Nazarov Cyclizations5.4	Diels–Alder Reactions6	Atropodiastereoselective Synthesis7	Conclusion
Cadaveric Evaluation of Myelinated Nerve Fiber Count in the Nerve to the Gracilis Muscle in Relation to Use as a Free Functional Muscle Transfer for Elbow Flexion
Authors: Mahmood Bilal , Marshall Danielle C. , Wolfe Scott W. , Lee Steve K. , Fufa Duretti T. 
Published: January 2020

Abstract
Background Optimizing axon count is essential for successful nerve transfer surgery, and a donor-to-recipient axon count ratio greater than 0.7:1 has been associated with improved outcomes. A gracilis free functioning muscle transfer (FFMT) is an option to restore elbow flexion, but its axon count has not been evaluated. Our aim was to quantify the axon count of the nerve to the gracilis muscle.
Methods The nerve to the gracilis was dissected in 10 fresh frozen adult cadaveric hindquarter specimens (four females and six males). The length of the nerve to the gracilis was measured and a biopsy taken. A validated histologic preparation technique was utilized, and axons were counted. The mean length and axon counts were calculated.
Results The average axon count in the nerve to the gracilis was 818 (range = 684–1,000, standard deviation [SD] = 116). The average length was 98 mm (range = 81–115 mm, SD = 13 mm).
Conclusion Our study found the average axon count in the nerve to the gracilis was 818. Prior literature suggests axon count ratio greater than 0.7:1 is associated with better clinical outcomes. Using data from prior studies, the spinal accessory, three intercostal, and two intercostal nerves are all sufficient for the transfer to the nerve to the gracilis with donor to recipient ratios of 1.7:1, 1.3:1, and 0.9:1, respectively.
Surgical Management and Outcomes of Aneurysms of Posterior Inferior Cerebellar Artery: Location-Based Approaches with Review of Literature
Authors: Deora Harsh , Nayak Nitish , Dixit Priyadarshi , Vikas V. , Rao K. V. L. Narasinga , Pruthi Nupur , Srinivas Dwarakanath , Shukla Dhaval P. , Bhat Dhananjay I. , Malla Bhaskara Rao , Devi Bhagvatula Indira , Somanna Sampath 
Published: January 2020

Abstract

Background Posterior inferior cerebellar artery (PICA) is a tortuous, variable, and uncommon site for aneurysms. Surgical management of PICA aneurysms involves careful selection of approach based on the location of the aneurysm and meticulous dissection of the neurovascular structures and perforators.

Materials and Methods We did a retrospective review of all the PICA aneurysms operated at our institute in the past 10 years along with the site, presentation, and approach used for the same. Preoperative World Federation of Neurosurgical Society scores and follow-up modified Rankin scores (mRS) were also evaluated. During the same period, data for intervention cases of PICA aneurysm were also collected with follow-ups for a comparative analysis.

Results A total of 20 patients with 21 PICA aneurysms were reviewed. All the reviewed cases presented with subarachnoid hemorrhage, and the most common location was the lateral medullary segment and vertebral artery (VA)–PICA junction. Midline approaches were used for distal PICA cases, with far-lateral approach reserved for anterior medullary/VA–PICA junction. No lower cranial nerve palsies were recorded at follow-up. Four cases needed cerebrospinal fluid diversion and two developed cerebellar infarcts. All cases were mRS 0 to 2 at follow-up.

Conclusion Our series compares well with some of the larger surgical series of PICA aneurysms. This may be due to early referral patterns and early surgery (<24 hours) policy at our institution. Anatomical knowledge of PICA anatomy and sound perioperative management are keys to good outcomes in these cases.
Mal de Debarquement Syndrome
Authors: Saha Kamala , Cha Yoon-Hee 
Published: January 2020

AbstractMal de debarquement syndrome (MdDS) is a disorder of persistent vertigo characterized by a feeling of oscillation such as rocking, bobbing, or swaying. It is triggered by passive motion, typically by exposure to water, air, or land transportation. This syndrome affects middle-aged individuals who are predominantly women. MdDS presents as a balance disorder that carries significant risk of morbidity due to both the direct effects of balance impairment and associated symptoms of fatigue, cognitive slowing, and visual motion intolerance. The Barany Society will be publishing criteria for diagnosing persistent MdDS. In addition, more insight has been gained into the pathophysiology of MdDS, with current hypotheses pointing to a cerebral and cerebellar basis. Treatments have expanded beyond medication trials, and now include the use of noninvasive brain stimulation and readaptation of the vestibulo-ocular reflex.
Risk Factors for Parenting Stress in Parents of Children Treated with Laser Surgery for Twin-Twin Transfusion Syndrome 2 Years Postpartum
Authors: Schifsky Kelly , Deavenport-Saman Alexis , Mamey Mary Rose , Sheth Nidhi , Mirzaian Christine B. , Schrager Sheree M. , Chmait Ramen H. , Vanderbilt Douglas L. 
Published: January 2020

Abstract
Objective Twin-twin transfusion syndrome (TTTS) is a rare but serious condition that can occur in monochorionic and diamniotic twin pregnancies. Research indicates almost half of postpartum mothers with TTTS may have clinically significant levels of stress. However, no studies have measured the levels of parenting stress at 2 years postpartum, and little research has been conducted on sources of stress.
Study Design A prospective cohort study was conducted. Data were collected from parents whose children were treated with laser ablation in utero and were 2 years old at the time of screening. The Parenting Stress Index - Short Form (PSI-SF) and its three subscales were used to collect data. Descriptive, bivariate, and multivariate analyses were conducted to determine significant predictors associated with each outcome.
Results A total of 99 children from 56 families were enrolled. Unmarried status (B = –22.8; p = 0.039) and lower maternal educational level (B = –7.8; p = 0.01) were both significantly associated with higher PSI-SF total score.
Conclusion Subgroups of parents whose pregnancy required laser surgery for TTTS may continue to have clinically significant levels of stress at 2 years postpartum. It is important the health care team is aware of this and the sociodemographic risk factors to provide appropriate support for families.
Vascular Injuries following Knee Dislocation
Authors: Stannard James P. , Schreiner Anna J. 
Published: January 2020

AbstractKnee dislocations (KDs) are frequently found in high-energy impact injuries, ranging from automobile accidents to contact sports. KDs require careful examination due to the limb-threatening nature of these injuries. A key examination in any KD comprises a proper assessment of the vascular status. The risk of popliteal artery injury with a KD has varied from 7 to 40%, with more contemporary studies reporting injury in the range of 7 to 15%. The notion of mandatory emergent arteriography was challenged in the trauma literature as several small retrospective studies suggested that selective arteriography was a safe and effective treatment protocol. New imaging modalities have evolved and have found their way into trauma surgery. Computed tomography angiography and magnetic resonance angiography are two contemporary imaging modalities that have different characteristics as well as availability. Arteriography has been the gold standard and is a reliable and proven method of evaluation. However, it has a number of negative aspects that must be considered when determining the ideal imaging for a patient following KD. Besides cost savings of about more than US$500,000, conventional arteriography, which comprises the use of X-ray, arterial puncture and cannulation, and the use of contrast agents containing iodine, has got a complication rate as high as 9% and more patient discomfort compared with the new imaging modalities. This clinical practice review documents that our algorithm of clinical examination first combined with advanced imaging in cases with abnormalities documented on examination is a safe and prudent policy in treating patients following KD.
A qualitative solution with quantitative potential for the mouse hippocampal cortex flatmap problem
Authors: Swanson Larry W. , Hahn Joel D. 
Published: January 2020

Significance
The hippocampal formation (HPF) plays an important role in many cerebral cortical functions, including memory consolidation, spatial navigation, and motivation and emotion. However, the highly convoluted hippocampal layers make visualization of basic spatial relationships unusually difficult. A mouse HPF flatmap was derived qualitatively from a physical model in rat, and to demonstrate its utility this flatmap was used to compare the results of experiments showing the intrahippocampal projections of dentate gyrus mossy fibers with Pcp4 gene expression. A spatial comparison of the rat and mouse HPF is included, and requirements are presented for producing a quantitative computer graphics flatmap that could be used to create a human hippocampal flatmap with accompanying coordinate system that transfers onto the intact brain.
Salicylic acid receptor NPR1 is involved in guard cell chitosan signaling
Authors: Prodhan Yeasin , Issak Mohammad , Munemasa Shintaro , Nakamura Yoshimasa , Murata Yoshiyuki 
Published: January 2020

Abstract

Chitosan (CHT) induces stomatal closure and thus plays a crucial role in plants to adapt to the adverse environments. Our previous results of a SA-deficient mutant nahG suggest that endogenous salicylic acid (SA) is involved in the CHT signaling in guard cells. Here in order to make the involvement definite, we examined stomatal responses to CHT of another SA-deficient mutant, sid2, and an SA receptor mutant, npr1-3. The sid2 mutation impaired CHT-induced stomatal closure and reactive oxygen species production and both impairments were complemented with exogenous SA application. Moreover, the CHT-induced stomatal closure is disrupted in the npr1-3 mutant. These results suggest that endogenous SA is involved in the CHT-induced stomatal closure via the SA receptor, NPR1.

Abbreviations
SA: salicylic acid; ABA: abscisic acid; ROS: reactive oxygen species; NPR1: nonexpresser of pathogenesis-related genes1; CHT: chitosan; DAB: 3,3′-diaminobenzidine
Characterization of Burkholderia sp. strain CJ1, a newly isolated berberine-degrading bacterium from rhizosphere of Coptis japonica
Authors: Yoshida Hinaka , Takeda Hisashi , Wakana Daigo , Hosoe Tomoo 
Published: January 2020

ABSTRACT
Burkholderia sp. strain CJ1 was newly isolated as berberine (BBR) degrading bacteria from rhizosphere of Coptis japonica. CJ1 had the ability to utilize BBR as the sole carbon source and revealed that BBR metabolism via 11-hydroxylation and demethylenation pathway. It was also revealed that the 11-hydroxylation ability of BBR and palmatine (PAL) has induced by BBR.
Observation of wide-angle impedance matching in terahertz photonic crystals
Authors: Li Shanshan , Wang Yu , Zhang Wenya , Lu Weixin , Hou Bo , Luo Jie , Lai Yun 
Published: January 2020

Abstract
Reflection due to impedance mismatch at interfaces of different materials is undesired in many THz applications including THz communication, spectroscopy and imaging. In this work, we theoretically and experimentally demonstrate a type of THz photonic crystals exhibiting wide-angle impedance matching property with free space. The reflection as well as the reflection-induced Fabry–Pérot resonances are efficiently suppressed in a relatively broad spectrum. A sample is fabricated using high-resistance silicon and polyethylene terephthalate films. By using THz time domain spectroscopy, we have clearly observed high transmittance and suppression of Fabry–Pérot resonances for a wide range of incident angles (0°–60°) and a relatively broad spectrum (0.26–0.55 THz) in both polarizations. Our work opens a general approach for improvement of transmission and imaging quality in THz spectroscopy.
The Perovskite Phase Optimize of Barium Titanate Nanoparticles
Authors: Ady Jan , Nurpratiwi Arum , Aliyah  , Apriliana Winda 
Published: January 2020

Abstract
The barium titanate gel has been made by sol-gel techniques and then sintered at 800°C for its nanoparticles powder. Previously, all of the samples are made into the mass ratio, particularly its notation in gram units to the composition with polyvinyl alcohol. Finally, its perovskite phase of the barium titanate nanoparticles were found and optimum occurs in the proportional mass ratio or BaTiO3/PVA. By X-ray diffraction equipment, it be examined to got the optimum tetragonal crystallite where it indicates the perovskite phase had been optimal formed. The both of PSA and TEM equipments were uses and found that barium titanate nanoparticles in which dominantly for all of them.
In vitro study of Nano Hydroxyapatite/Streptomycin -Gelatin-Based Injectable Bone Substitute Associated- 3D printed Bone Scaffold for Spinal Tuberculosis Case
Authors: Firdhausi Wardhani Inten , Mega Rizki Samudra Rofi , Katherine  , Hikmawati Dyah 
Published: January 2020

Abstract
According to WHO (2018) Indonesia was placed in the third ranked of the highest number of TB sufferers worldwide. TB was caused by the Mycobacterium tuberculosis. This bacterial infection not only could affect the lungs, but also bone tissue, especially the spine, which then called as spinal tuberculosis. There had been many alternatives to overcome this case. One of them was by using Injectable Bone Substitutes (IBS) based on nano Hydroxyapatite (nanoHA), gelatin and streptomycin. The severe sponal tuberculosis case might cause the bone defect. Due to this bone defect, IBS alone would not be able to handle this issue. Thus, scaffold was needed to replace and support the infected bone. Besides, the combination between IBS paste and bone scaffold could help the bone tissue regeneration. The nanoHA and gelatin contents of the IBS paste provided the mineral which was need for bone tissue regeneration. While the bone scaffold would support those contents by providing the extracellular matrix for the new bone tissue attachment. The IBS associated- scaffold could be an alternative to deliver spinal tuberculosis’ drug locally. This study aimed to evaluate the performance of this combination through antibacterial study and drug release test using UVVis spectrophotometer. As well as the cytotoxicity test to know the risk of these two materials within the body. The IBS was synthesized by mixing nano hydroxyapatite and 20 w/v% gelatin with ratio of 65:35 and 10% streptomycin addition as antibiotic agent. The mixture was added by hydroxypropyl methylcellulose as suspending agent. FTIR test showed that there was a chemical reaction occurred in the mixture. The performance of this combination showed that the released- streptomycin, qualitatively, could kill the Staphylococcus aureus trough the antibacterial test. Furthermore, it also had been proven that the IBS associated- scaffold could release an increasing streptomycin concentration over time. In addition, the combination showed a good result in cytotoxicity test where the cell viability was more than 50%. According to the results mentioned before, the IBS paste associated- scaffold was a promising breakthrough to overcome spinal tuberculosis case as a local drug deliver.