bluuebunny/crossref_metadata_2025_split huggingface dataset:
An ancient viral epidemic involving host coronavirus interacting genes more than 20,000 years ago in East Asia
Authors: Souilmi Yassine , Lauterbur M. Elise , Tobler Ray , Huber Christian D. , Johar Angad S. , Enard David 
Published: November 2020

SummaryThe current SARS-CoV-2 pandemic has emphasized the vulnerability of human populations to novel viral pressures, despite the vast array of epidemiological and biomedical tools now available. Notably, modern human genomes contain evolutionary information tracing back tens of thousands of years, which may help identify the viruses that have impacted our ancestors – pointing to which viruses have future pandemic potential. Here, we apply evolutionary analyses to human genomic datasets to recover selection events involving tens of human genes that interact with coronaviruses, including SARS-CoV-2, that likely started more than 20,000 years ago. These adaptive events were limited to the population ancestral to East Asian populations. Multiple lines of functional evidence support an ancient viral selective pressure, and East Asia is the geographical origin of several modern coronavirus epidemics. An arms race with an ancient coronavirus, or with a different virus that happened to use similar interactions as coronaviruses with human hosts, may thus have taken place in ancestral East Asian populations. By learning more about our ancient viral foes, our study highlights the promise of evolutionary information to better predict the pandemics of the future. Importantly, adaptation to ancient viral epidemics in specific human populations does not necessarily imply any difference in genetic susceptibility between different human populations, and the current evidence points toward an overwhelming impact of socioeconomic factors in the case of COVID-19.
Examining the Persistence of Human Coronaviruses on Fresh Produce
Authors: Blondin-Brosseau Madeleine , Harlow Jennifer , Doctor Tanushka , Nasheri Neda 
Published: November 2020

AbstractHuman coronaviruses (HCoVs) are mainly associated with respiratory infections. However, there is evidence that highly pathogenic HCoVs, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Middle East Respiratory Syndrome (MERS-CoV), infect the gastrointestinal (GI) tract and are shed in the fecal matter of the infected individuals. These observations have raised questions regarding the possibility of fecal-oral route as well as foodborne transmission of SARS-CoV-2 and MERS-CoV. Studies regarding the survival of HCoVs on inanimate surfaces demonstrate that these viruses can remain infectious for hours to days, however, to date, there is no data regarding the viral survival on fresh produce, which is usually consumed raw or with minimal heat processing. To address this knowledge gap, we examined the persistence of HCoV-229E, as a surrogate for highly pathogenic HCoVs, on the surface of commonly consumed fresh produce, including: apples, tomatoes and cucumbers. Herein, we demonstrated that viral infectivity declines within a few hours post-inoculation (p.i) on apples and tomatoes, and no infectious virus was detected at 24h p.i, while the virus persists in infectious form for 72h p.i on cucumbers. The stability of viral RNA was examined by droplet-digital RT-PCR (ddRT-PCR), and it was observed that there is no considerable reduction in viral RNA within 72h p.i.
The metabolic pace of life histories across fishes
Authors: Wong Serena , Bigman Jennifer S. , Dulvy Nicholas K. 
Published: November 2020

AbstractAll life acquires energy through metabolic processes and that energy is subsequently allocated to life-sustaining functions such as survival, growth, and reproduction. Thus, it has long been assumed that metabolic rate is related to the life history of an organism. Indeed, metabolic rate is commonly believed to set the pace of life by determining where an organism is situated along a fast-slow life history continuum. However, empirical evidence of a relationship between metabolic rate and life histories is lacking, especially for ectothermic organisms. Here, we ask whether three life history traits – maximum body mass, generation length, and growth performance – explain variation in resting metabolic rate (RMR) across fishes. We found that growth performance, which accounts for the trade-off between growth rate and maximum body size, explained variation in RMR, yet maximum body mass and generation length did not. Our results suggest that measures of life history that encompass trade-offs between life history traits, rather than traits in isolation, explain variation in RMR across fishes. Ultimately, understanding the relationship between metabolic rate and life history is crucial to metabolic ecology and has the potential to improve prediction of the ecological risk of data-poor species.
Multiscale Invasion Assay for Probing Macrophage Response to Bacteria
Authors: Wodzanowski Kimberly A. , Kloxin April M. , Grimes Catherine L. 
Published: November 2020

AbstractThe immune system is a complex network of various cellular components that must differentiate between pathogenic bacteria and the commensal bacteria of the human microbiome, where misrecognition is linked to inflammatory disorders. Fragments of bacterial cell wall peptidoglycan bind to pattern recognition receptors within macrophages, leading to immune activation. To study this complex process, an approach for three-dimensional (3D) culture of human macrophages and their invasion with relevant bacteria in a well-defined hydrogel-based synthetic matrix inspired by the gut was established. Workflows were developed for monocyte encapsulation and differentiation into macrophages in 3D culture with high viability. Bacteria invaded into macrophages permittedin situpeptidoglycan labeling. Macrophages exhibited biologically-relevant cytokine release in response to bacteria. This multi-dimensional bacteria-macrophage co-culture system will prove useful in future studies to observe bacterial fragment production and localization in the cell at the carbohydrate level for insights into how our immune system properly senses bacteria.TOC Figure
Revisiting the Notion of Deleterious Sweeps
Authors: Johri Parul , Charlesworth Brian , Howell Emma K. , Lynch Michael , Jensen Jeffrey D. 
Published: November 2020

ABSTRACTIt has previously been shown that, conditional on its fixation, the time to fixation of a semi-dominant deleterious autosomal mutation in a randomly mating population is the same as that of an advantageous mutation. This result implies that deleterious mutations could generate selective sweep-like effects. Although their fixation probabilities greatly differ, the much larger input of deleterious relative to beneficial mutations suggests that this phenomenon could be important. We here examine how the fixation of mildly deleterious mutations affects levels and patterns of polymorphism at linked sites - both in the presence and absence of interference amongst deleterious mutations - and how this class of sites may contribute to divergence between-populations and species. We find that, while deleterious fixations are unlikely to represent a significant proportion of outliers in polymorphism-based genomic scans within populations, minor shifts in the frequencies of deleterious mutations can influence the proportions of private variants and the value ofFSTafter a recent population split. As sites subject to deleterious mutations are necessarily found in functional genomic regions, interpretations in terms of recurrent positive selection may require reconsideration.
Computer Designed PRC2 Inhibitor, EBdCas9, Reveals Functional TATA boxes in Distal Promoter Regions
Authors: Levy Shiri , Somasundaram Logesh , Raj Infencia Xavier , Ic-Mex Diego , Schmidt Sven , Alghadeer Ammar , Honkanen Henrik , Hawkins R. David , Mathieu Julie , Wang Yuliang , Baker David , Bomsztyk Karol , Ruohola-Baker Hannele 
Published: November 2020

SummaryThe critical process in development, bifurcation of cellular fates, requires epigenetic H3K27me3 marks propagated by PRC2 complex. However, the precise chromatin loci of functional H3K27me3 marks are not yet known. Here we identify critical PRC2 functional sites at a single nucleosome resolution. We fused a computationally designed protein, EED binder (EB) that competes with EZH2 and thereby disrupts PRC2 function, to dCas9 (EBdCas9) to direct PRC2 inhibition at a precise locus using gRNA. We targeted EBdCas9 to 4 different genes (TBX18, p16, CDX2 and GATA3) and observed epigenetic remodeling at a single nucleosome resolution resulting in gene activation. Remarkably, while traditional TATA box is located 30bp upstream of TSS, we identified a functional TATA box, >500bp of TSS, normally repressed by PRC2 complex. Deletion of this TATA box eliminates EBdCas9 dependent TBP recruitment and transcriptional activation. Targeting EBdCas9 to CDX2 and GATA3 results in trophoblast trans-differentiation. EBdCas9 technology is broadly applicable for epigenetic regulation at a single locus to control gene expression.Graphical Abstract
Proteomic analysis reveals Utf1 as a neurogenesis-associated new Sumo target
Authors: Correa-Vázquez Juan F. , Juárez-Vicente Francisco , García-Gutiérrez Pablo , Barysch Sina V. , Melchior Frauke , García-Domínguez Mario 
Published: November 2020

AbstractPost-translational modification by covalent attachment of Sumo regulates numerous processes in vertebrates. Despite demonstrated roles of Sumo in development and function of the nervous system, the identification of key factors displaying a sumoylation-dependent activity during neurogenesis remains elusive. Based on SILAC, we have identified the Sumo proteome of proliferating and neuronal-differentiating cells. More than 300 putative Sumo targets differentially associated with one or the other condition. Among these, Utf1 revealed as a new Sumo target. Gain-of-function experiments demonstrated marked differences between the effects on neurogenesis of wild type and sumoylation mutant versions of selected proteins. While sumoylation of Prox1, Sall4a, Trim24 and Utf1 associated with a positive effect on neurogenesis, sumoylation of Kctd15 associated with a negative effect. Similar results were observed in embryos. Finally, detailed analysis of Utf1 showed sumoylation-dependent control of bivalent genes expression. This effect relies on two mechanisms: sumoylation modulates Utf1 chromatin binding and mediates recruitment of the mRNA-decapping enzyme Dcp1a through a conserved SIM. Altogether, our results indicate that combined sumoylation status of key proteins determine proper progress of neurogenesis.Sumo/transcription/chromatin/neurogenesis/Utf1
Investigating the antecedents of e-commerce satisfaction in social commerce context
Authors: Attar Razaz Waheeb , Shanmugam Mohana , Hajli Nick 
Published: November 2020

PurposeSocial media is still influencing consumers and is extending social commerce (S-Commerce) use. Different social media activities can influence the users' trust and e-satisfaction at different levels, which in turn influence the purchase intentions. This is evident for the food and beverage industry as S-Commerce mediated by social media can help realise a shorter time to market and meet buyer demands. In addition, credibility factors may influence trust and purchase intentions. Understanding the various factors of influence such as social constructs, namely ratings, reviews and referrals; design constructs such as credibility and features and behavioural constructs such as trust, satisfaction and motivation; and analysing the relationship between these factors and how they influence purchase intentions can provide deeper insights into S-Commerce research, decision-making process and purchase intentions particularly from a food and beverage context.Design/methodology/approachDrawing on trust through social media activities and surface credibility as well as e-commerce satisfaction, the authors have proposed a research model to investigate the purchase intention of consumers in S-Commerce platforms. Survey data were collected from six countries in Asia and analysed using SEM-PLS.FindingsResults indicated that both trust and surface credibility significantly influence e-commerce satisfaction leading to purchase intention. Furthermore, surface credibility, which is a novel predictor for purchase intention in S-Commerce context, is highly significant on e-commerce satisfaction. Besides, encouraged by surface credibility, it was identified that trust significantly affects e-commerce satisfaction and results in purchase intention. This research adds contribution to theory and practice in S-Commerce stream as discussed at the end of the paper.Originality/valueThe results of this research contribute to the S-Commerce literature and have practical implications for practitioners in the food and beverage industry. As such, focussing on these constructs, this paper analyses the relationship between the social media activities, trust, e-commerce satisfaction, surface credibility and intention to buy.
Higher education in Japan: internationalization, the Sustainable Development Goals and survivability
Authors: Edwards Sachi , Ashida Akemi 
Published: November 2020

PurposeThis paper reviews the national and institutional internationalization activities in Japan's higher education sector and considers the extent to which these efforts have attempted to incorporate and/or contribute to meeting the Sustainable Development Goals (SDGs).Design/methodology/approachThis paper was developed based on a review of available demographic data on internationalization in Japan (in both English and Japanese), a survey of recent scholarly literature on this topic and conversations with numerous faculty and staff members working on internationalization issues in a wide range of higher education institutions throughout the country.FindingsThere are substantial internationalization efforts being made at both national and institutional levels, yet scholars and practitioners of higher education question the extent to which genuine internationalization is occurring. Moreover, the metrics used to track internationalization are somewhat limited and the available data, in many cases, can be complicated to interpret. A bit of tension also exists in Japanese universities between those who support the movement to internationalize and those who see it as a passing fad, an intrusion on their academic freedom and/or as a guise for Westernization – a tension that some cite, along with language barriers and system misalignment, as a challenge to internationalization.Originality/valueNumerous scholars discuss the internationalization of higher education in Japan. The originality of this paper is in the comparison of Japan's higher education internationalization efforts to the movement to achieve the SDGs – both in Japan and as a global effort.
Organizational change and job insecurity: the moderating role of employability
Authors: Çalışkan Nurgül , Özkoç Aziz Gökhan 
Published: November 2020

PurposeThe purpose of this study is to examine the relationship between characteristics of change (frequency of change [FC], impact of change [IC], planning involved in change [PC]) and job insecurity (JIS), as well as the moderating role of employability (EMP) within these relationships.Design/methodology/approachData were obtained from a sample of 361 permanent employees working at four- and five-star hotels. The hypothesized relationships were tested via structural equation modeling.FindingsThe findings revealed that employees’ perceptions of JIS are affected positively by FC and IC and negatively by PC. Additionally, this study confirmed that the perception of EMP moderates the relationships between the characteristics of organizational change (FC, IC, PC) and JIS.Research limitations/implicationsNo causal inference can be made with regard to the relationships in this study by virtue of the cross-sectional data used in the study. Hence, further studies with longitudinal design and wider samples covering different regions and/or tourism destinations need to be carried out to validate the results of this study and to further clarify the direction of the relationships presented here.Originality/valueTo the best of authors’ knowledge, this study presents the first empirical evidence on the moderating role of EMP in accounting for the JIS resulting from FC, IC and PC, the characteristics of organizational change. Also, the significance of this study lies in the fact that its subject has not been addressed before from the perspective of the hospitality industry.
The behavioral analysis of choice difficulty states during clothing online shopping
Authors: Chen Yumiao , Yang Zhongliang 
Published: November 2020

PurposeChoice overload in e-commerce induces choice difficulty, which is detrimental to shopping decision-making. The purpose of this paper is to provide relatively simple and effective methods and indicators to detect and assess the choice difficulty states of customers during clothing online shopping.Design/methodology/approachIn order to find out the behavioral performance of choice difficulty states during clothing online shopping, the authors performed the following steps: at first, the authors conducted an experiment to record the videos of the purchasing process during clothing online shopping. Then, the authors carried out the behavioral analysis of customers, correlating each behavioral index to choice difficulty states.FindingsThe results of the behavioral analysis in this study have indicated that three types of behavior were significantly correlated to the choice difficulty states of customers.Practical implicationsIt is expected that it would be possible to use the threshold values of behavioral indicators to determine whether and when the customer is suffering from choice phobia disorder. Based on the findings, a recommender system with timely interventions to help customers with choice difficulty to make shopping decisions will be developed in the future.Originality/valueThis is the first reported study that explores the possibility of using behavioral indicators to detect choice difficulty, which will remain helpful to the scientific community to start further customer-centered research work and development of clothing online shopping.
Sustainability of Brazilian public debt: a structural break analysis
Authors: Campos Eduardo Lima , Cysne Rubens Penha 
Published: November 2020

PurposeThe objective of this work is to investigate the existence of structural breaks in multicointegration models estimated for Brazilian fiscal variables and to identify their effects on the sustainability or not of this country`s fiscal policy between December 1997 and June 2018.Design/methodology/approachThe authors apply the econometric multicointegration method, in order to analyze long-term relationships between accumulated revenue and expenses of Brazilian central government and the stock of its debt, incorporating structural breaks, over the study period.FindingsThe unsustainability of the debt/GDP ratio is found here, as in a previous work, but now considering a structural break. As one of the contributions, the present work makes it possible to identify the date as of which Brazilian fiscal policy may have become unsustainable: May 2014.Originality/valueThe work points out the worsening of Brazilian fiscal situation as of 2014. The authors adapted original methodologies both in model specification and in the stationarity test used. The estimated parameters before and after structural break allow for identifying changes in fiscal variables that may have led to unsustainability, thus providing possible guidance for fiscal policy.
The impact of ownership structure and the board of directors’ composition on tax avoidance strategies: empirical evidence from Jordan
Authors: Alkurdi Amneh , Mardini Ghassan H. 
Published: November 2020

PurposeAdopting agency theory, the purpose of this study is to explore the impact of ownership structure and board of directors’ composition on the extent of tax avoidance strategies.Design/methodology/approachThe sample included all of the Jordanian first market companies listed on the Amman Stock Exchange from 2012 to 2017, comprising 348 observations.FindingsThe main finding of the paper is that tax avoidance is negatively related to managerial and institution ownership structures, which reduces the usage of tax avoidance strategies. Foreign ownership, however, has a positive relation that increases the likelihood of adopting tax avoidance strategies.Practical implicationsThis study has policy implications for policymakers in relation to designing future tax systems to reduce the possibility of engaging in tax avoidance practices.Originality/valueTo the best of the authors’ knowledge, this study is the first of its kind that investigates the effects of the managerial, foreign and institutional ownership classes and board composition on tax avoidance for Jordanian listed companies.
The application of social innovation as it relates to older people and the implications for future policymaking: a scoping review
Authors: Crossen-White Holly Louise , Hemingway Ann , Ladkin Adele 
Published: November 2020

Purpose
Social innovation has received increasing attention in recent decades (Agostini et al., 2017). This study aims to consider how the concept has been applied to the issue of ageing and what can be learnt about effective policy responses.

Design/methodology/approach
The acknowledged lack of understanding generally about the concept makes it timely to undertake a scoping review of the current evidence from social innovation projects associated with older people. A scoping review is considered appropriate where there is a need to “identify and analyse knowledge gaps” (Munn et al., 2018, p. 2).

Findings
Findings from the scoping review indicate that, as yet, the concept of social innovation is not fully defined. However, it has widespread appeal across a diverse range of disciplines and has the potential to generate innovative policy responses.

Originality/value
A key argument identified is the need to change the public’s perceptions of ageing and devise public policies that encourage and nurture age-friendly communities. In summation, although social innovation has the potential to act as a policy driver, but to be effective, it is necessary to devise robust strategies to ensure full user-engagement and active involvement of communities. Therefore, it is the process of delivery that needs urgent attention in any future research into social innovation.
Stronger together? Intergenerational connection and Covid-19
Authors: Burke Stephen 
Published: November 2020

Purpose
This paper aims to review how intergenerational connections and relationships have been affected to date by COVID-19. It provides lessons for the future.

Design/methodology/approach
This paper is a review of policy and practice.

Findings
Although there are some excellent examples of creative approaches such as online strategies to bring generations together in the face of social distancing, there remain barriers to building stronger communities. Many people of all ages remain lonely and isolated. Community projects are under-funded and will struggle to maintain connections beyond the immediate crisis. Inequalities and the digital divide have been exacerbated by COVID-19. Intergenerational relations are likely to be further strained by the economic impact.

Originality/value
None of us have known anything like COVID-19 and its impact on all aspects of our lives. It will continue to affect generations to come, and we need to learn the lessons as we move forward.