bluuebunny/crossref_metadata_2025_split huggingface dataset:
SIRT1 Decreases Emotional Pain Vulnerability with Associated CaMKIIα Deacetylation in Central Amygdala
Authors: Zhou Chenghua , Wu Yuqing , Ding Xiaobao , Shi Naihao , Cai Youqin , Pan Zhizhong Z. 
Published: January 2020

Emotional disorders are common comorbid conditions that further exacerbate the severity and chronicity of chronic pain. However, individuals show considerable vulnerability to the development of chronic pain under similar pain conditions. In this study on male rat and mouse models of chronic neuropathic pain, we identify the histone deacetylase Sirtuin 1 (SIRT1) in central amygdala as a key epigenetic regulator that controls the development of comorbid emotional disorders underlying the individual vulnerability to chronic pain. We found that animals that were vulnerable to developing behaviors of anxiety and depression under the pain condition displayed reduced SIRT1 protein levels in central amygdala, but not those animals resistant to the emotional disorders. Viral overexpression of local SIRT1 reversed this vulnerability, but viral knockdown of local SIRT1 mimicked the pain effect, eliciting the pain vulnerability in pain-free animals. The SIRT1 action was associated with CaMKIIα downregulation and deacetylation of histone H3 lysine 9 at theCaMKIIα promoter. These results suggest that, by transcriptional repression ofCaMKIIα in central amygdala, SIRT1 functions to guard against the emotional pain vulnerability under chronic pain conditions. This study indicates that SIRT1 may serve as a potential therapeutic molecule for individualized treatment of chronic pain with vulnerable emotional disorders.SIGNIFICANCE STATEMENTChronic pain is a prevalent neurological disease with no effective treatment at present. Pain patients display considerably variable vulnerability to developing chronic pain, indicating individual-based molecular mechanisms underlying the pain vulnerability, which is hardly addressed in current preclinical research. In this study, we have identified the histone deacetylase Sirtuin 1 (SIRT1) as a key regulator that controls this pain vulnerability. This study reveals that the SIRT1–CaMKIIaα pathway in central amygdala acts as an epigenetic mechanism that guards against the development of comorbid emotional disorders under chronic pain, and that its dysfunction causes increased vulnerability to the development of chronic pain. These findings suggest that SIRT1 activators may be used in a novel therapeutic approach for individual-based treatment of chronic pain.
Marked Neurospora crassa Strains for Competition Experiments and Bayesian Methods for Fitness Estimates
Authors: Kronholm Ilkka , Ormsby Tereza , McNaught Kevin J , Selker Eric U , Ketola Tarmo 
Published: January 2020

Abstract
The filamentous fungus Neurospora crassa, a model microbial eukaryote, has a life cycle with many features that make it suitable for studying experimental evolution. However, it has lacked a general tool for estimating relative fitness of different strains in competition experiments. To remedy this need, we constructed N. crassa strains that contain a modified csr-1 locus and developed an assay for detecting the proportion of the marked strain using a post PCR high resolution melting assay. DNA extraction from spore samples can be performed on 96-well plates, followed by a PCR step, which allows many samples to be processed with ease. Furthermore, we suggest a Bayesian approach for estimating relative fitness from competition experiments that takes into account the uncertainty in measured strain proportions. We show that there is a fitness effect of the mating type locus, as mating type mat a has a higher competitive fitness than mat A. The csr-1* marker also has a small fitness effect, but is still a suitable marker for competition experiments. As a proof of concept, we estimate the fitness effect of the qde-2 mutation, a gene in the RNA interference pathway, and show that its competitive fitness is lower than what would be expected from its mycelial growth rate alone.
Fetal cardiovascular alterations in twin-to-twin transfusion syndrome
Authors: Rotar Ioana Cristina , Zaharie Gabriela , Staicu Adelina , Preda Andreia , Mureșan Daniel 
Published: January 2020

Twin-to-twin transfusion syndrome (TTTS) is the consequence of vascular anastomoses of the shared placenta of monochorionic twin pregnancies. Both circulating inter-twin blood flow and vasoactive mediators imbalance cause hypovolemia in the donor and hypervolemia in the recipient fetus. If left untreated, TTTS has a high perinatal mortality rate and adverse long-term outcomes mainly cardiovascular and neurological.
The recipient has cardiovascular changes including atrioventricular valve regurgitation, diastolic dysfunction and pulmonary stenosis/atresia. The maladaptive response to vascular changes determines a constant decreased blood flow in the donor that permanently modifies the arterial structure leading to postnatal alterations in the vascular system. Fetoscopic LASER surgery of placental vascular anastomoses may disrupt the underlying pathophysiology and improves cardiovascular function with normalization of systolic and diastolic function within weeks after treatment.
The impact of cardiovascular changes is relevant for the safety of the management of a TTTS case. The improvement of the perinatal survival after intrauterine surgery leads to viable infants with the longer-term sequelae. Therefore accurate quantification of cardiovascular involvement is essential for clinicians for pregnancy management but also for patient counseling about the potential treatment options the outcome.
Interfacility Transfers Among Patients With Complex Chronic Conditions
Authors: White Michelle J. , Sutton Ashley G. , Ritter Victor , Fine Jason , Chase Lindsay 
Published: January 2020

OBJECTIVES:To describe interfacility transfers among children with complex chronic conditions (CCCs) and determine if interfacility transfer was associated with health outcomes. We hypothesized that interfacility transfer would be associated with length of stay (LOS), receipt of critical care services, and in-hospital mortality.METHODS:In this retrospective cohort study, we used data from the 2012 Kids’ Inpatient Database. CCC hospitalizations were identified by International Classification of Diseases, Ninth Revision codes. Receipt of critical care services was inferred by using International Classification of Diseases, Ninth Revision diagnosis and procedure codes. We performed a descriptive analysis of CCC hospitalizations then determined if transfer was associated with LOS, mortality, or receipt of critical care services using survey-adapted quasi-Poisson or logistic regression models, controlling for hospital and patient demographics.RESULTS:There were 551 974 non–birth hospitalizations with at least 1 CCC diagnosis code. Of these, 13% involved an interfacility transfer. Compared with patients with CCCs who were not transferred, patients with CCCs who were transferred in and ultimately discharged from the receiving hospital had an adjusted LOS rate ratio of 1.6 (95% confidence interval [CI]: 1.5–1.7; P &lt; .001), were more likely to have received critical care services (adjusted odds ratio 3.0; 95% CI: 2.7–3.2; P &lt; .001), and had higher in-hospital mortality (adjusted odds ratio 3.6; 95% CI: 3.2–3.9; P &lt; .001) (controlling for patient and hospital characteristics).CONCLUSIONS:Many hospitalizations for children with CCCs involve interfacility transfer. Compared with in-house admissions, hospitalizations of patients who are transferred in and ultimately discharged from the receiving hospital involve longer LOS, greater odds of receipt of critical care services, and in-hospital mortality. Further evaluation of the role of clinical and transfer logistic factors is needed to improve outcomes.
Variation in Treatment and Outcomes of Children With Acute Disseminated Encephalomyelitis
Authors: Press Craig A. , Kirschen Matthew , LaRovere Kerri , Risen Sarah , Guilliams Kristin P. , Chung Melissa , Griffith Jennifer , Erklauer Jennifer , Peariso Katrina , Ducharme-Crevier Laurence , Shah Samir S. , Hall Matt , Wainwright Mark S. 
Published: January 2020

OBJECTIVES:
To characterize variation in treatments and outcomes of pediatric patients admitted to children’s hospitals with acute disseminated encephalomyelitis (ADEM).

METHODS:
In this retrospective cohort study, we used data from the Pediatric Health Information System. Children &gt;30 days old who were hospitalized from 2010 to 2015 with ADEM were included. Variables analyzed were treatments and admission to an ICU. Primary outcomes were discharge disposition and readmissions for relapses (ADEM readmissions) or for continued comorbidities (non-ADEM readmissions).

RESULTS:
A total of 954 patients with ADEM had 1117 admissions. Treatments included steroids (80%), immunoglobulin (22%), and plasmapheresis (6.6%); 15% of admissions included none of these treatments. Treatments varied by center (P &lt; .001). Thirty-four percent of admissions included ICU admission, which was associated with an increased number and duration of treatments (P &lt; .01). The discharge disposition was home in 85% of admissions; home with health services, rehab facility, or other in 13.6%; and mortality in 1.4%. Twelve percent (117 of 954) of patients had &gt;1 admission for ADEM. Treatment choice and ICU stay were not associated with ADEM readmissions. Sixteen percent (181 of 1101) of ADEM admissions had a non-ADEM readmission within 90 days. Prolonged ICU hospitalization was associated with non-ADEM readmission (adjusted odds ratio 1.9; P = .017) and decreased likelihood of discharge from the hospital to home (adjusted odds ratio 0.1; P &lt; .001). After adjusting for ICU duration, treatment choice and duration were not associated with non-ADEM readmission or hospital disposition.

CONCLUSIONS:
Significant variation in ADEM treatment exists across centers. Admission to an ICU for ADEM was associated with increased immunotherapy, additional health services at discharge, and readmission for diagnoses other than ADEM.
Taking the Pain out of Somatization: Development and Implementation of a Hospital-Based Clinical Practice Guideline to Address Pediatric Somatic Symptom and Related Disorders
Authors: Kullgren Kristin A. , Shefler Alanna , Malas Nasuh , Monroe Kimberly , Leber Steven M. , Sroufe Nicole , El Sakr Ashleigh , Pomeranz Elaine , O’Brien Edmond , Mychaliska Kerry P. 
Published: January 2020

OBJECTIVES:
The diagnostic category of somatic symptom and related disorders (SSRDs), although common, is often poorly recognized and suboptimally managed in inpatient pediatric care. Little literature exists to address SSRDs in the inpatient pediatric setting. The purpose of the study was to characterize current SSRD practice, identify problem areas in workflow, and develop a standardized approach to inpatient evaluation and management at a tertiary care academic children’s hospital.

METHODS:
A multidisciplinary group identified patients with SSRD admitted between May 2012 and October 2014. A retrospective chart review on a convenience sample was performed to identify population characteristics and current practice. Lean methodology was used to define current state practice and future state intervention. These methods were used to guide identification of problem areas, which informed protocol, a clinical practice guideline, and resource development.

RESULTS:
Thirty-six patients aged 8 to 17 years met inclusion criteria for chart review. Most patients presented with either neurologic or pain-related complaints. The mean length of stay was 5.44 days (SD = 6.3), with few patients receiving a mental health consultation within 24 hours of hospitalization. Patients averaged 5.8 medical and/or psychiatric diagnoses on discharge (SD = 5.2), and two-thirds did not have an SSRD diagnosis. Half of patients had comorbid psychiatric diagnoses, whereas one-quarter were discharged with no mental health follow-up.

CONCLUSIONS:
In this study, we describe the process and content development of a single-site institutional protocol, clinical practice guideline, and resources for the evaluation and management of pediatric SSRDs. This study may serve as a model for similar standardization of SSRD care in other inpatient pediatric medical settings.
Performance of Cellular Neural Network Based Channel Equalizers
Authors: ÖZMEN Atilla , TANDER Baran , ŞENOL Habib 
Published: January 2020

Abstract—In this paper, a popular dynamic neural network structure called Cellular Neural Network (CNN) is employed as a channel equalizer in digital communications. It is shown that, this nonlinear system is capable of suppressing the effect of intersymbol interference (ISI) and the noise at the channel. The architecture is a small-scaled, simple neural network containing only 25 neurons (cells) with a neighborhood of r = 2 , thus including only 51 weight coefficients. Furthermore, a special technique called repetitive codes in equalization process is also applied to the mentioned CNN based system to show that the two-dimensional structure of CNN is capable of processing such signals, where performance improvement is observed. Simulations are carried out to compare the proposed structures with minimum mean square error (MMSE) and multilayer perceptron (MLP) based equalizers.
The opinion of farmers and small and medium-sized enterprises on the importance of ICT in Hajdú-Bihar County, Hungary
Authors: Boros-Papp Sándor , Várallyai László 
Published: January 2020

The purpose of this study is to gain a better understanding, conceptualization, and measurement about the usage of ICT among SMEs that could enhance and transform their business to be more efficient and resilient. A more specific aim is to explore the state of ICT adoption as well as to examine the drivers and barriers of digital transformation through the lens of SMEs. In the primary (quantitative) phase of our research, we conducted a questionnaire survey among micro-enterprises and primary producers on the use of ICT in Hajdú-Bihar County, where 237 people answered the questionnaire. In all cases, the respondent was listed as a primary producer or in a joint primary producer certificate.
We were investigated which tools are using in communication with suppliers, customers and government organisations, which type of cloud services are used and the safe storage by Internet Service Providers. We get about the same results in the case of the suppliers, customers. In the case of the governmental institutions, Internet usage was bigger, because of a significant number of them require national or EU grants, which can be submitted electronically. The secure data storage is the safest possible for the farmers.
Correlation between severity of health status in extremely premature newborns on third day of life and clinicolaboratory indices
Authors: Furman E. G. , Nikolenko A. V. , Kulizhnikov G. V. 
Published: January 2020

Aim. To detect the correlation between the severity of health status in extremely premature newborns and the changes in clinical and laboratory indices.
Materials and methods. The study included small premature newborn infants with extremely low body mass and very low-birth-weight infants with clinical picture of respiratory distress syndrome. To carry out the study, patients were divided into 2 groups depending on the health status severity index on the third day of life using NEOMOD scale. Group I enclosed infants with health status severity on the third day of life from 0 to 2 scores, group II patients with health status severity on the third day from 3 and more scores by NEOMOD scale.
Results. The patients with more severe health status according to NEOMOD scale had lower blood erythrocyte index on the first day of life as compared to the patients with less severe health status (U = 64; р = 0.034). There was revealed a direct relationship between health status severity on the third day and health status severity on the first day (U = 56.5; р = 0.014), stab neutrophil number (U = 57; р = 0.016) and neutrophil nuclear shift index (NNSI) (U = 61; p = 0.025) on the twenty eighth day.
Conclusions. The lower number of erythrocyte on the first day of life is interconnected with more severe health status on the third day of life. The initial severity of respiratory failure in case of respiratory distress syndrome influences the duration of infectious processes, in particular, pneumonia with high indices of infectious markers.
Prevalence and structure of foot deformities in primary school children
Authors: Averyanova N. I. , Pristupova V. V. , Semerikova A. I. , Starkova I. L. 
Published: January 2020

Aim. To study the prevalence and structure of deformity of the foot in primary school children (pupils of the 1st- 4th grades).
Materials and methods. The study involved 277 children aged 7-10 years. The main method of investigation used was computed plantography by means of electronic baropodometer PadProfessional.
Results. Deformity of the foot was detected in 58% of children 65.3% of boys and 52.3% of girls (p 0.05). The following pathology was revealed: platypodia, increase in the load on the calcaneal part of the foot and malposition of the foot. The most common pathology was platypodia diagnosed in 28.2% of children.
Conclusions. High prevalence of foot deformities requires their early diagnosis and correction as well as improvement of parents knowledge regarding this problem.
Comparison of the Effects of Soy Protein and Whey Protein Supplementation during Exercise: a Systematic Review
Authors: Silva Suelen Maiara Medeiros da , Carminati Bárbara Cristovão , Santos Junior Valfredo De Almeida , Lollo Pablo Christiano Barboza 
Published: January 2020

AbstractThe interest of the supplementation market for the soy protein consumption to optimize physical and metabolic performance after exercise is increasing. However, evidence suggests that the soy protein ingestion has lower anabolic properties when compared with whey protein. The purpose of this systematic review was to compare the effects of whey protein and soy protein supplementation on the muscle functions maintenance after exercise. This review was performed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Articles were searched for in the Pubmed database and included studies comparing the effects of soy protein and whey protein consumption on protein synthesis, lean mass gain and oxidative stress reduction in response to endurance or resistance training. Thirteen trials were included in this review. The results showed that the whey protein consumption is superior to that of soy protein with respect to protein synthesis and lean mass gain, but soy protein showed superior results in reducing oxidative stress. Future research comparing both soy and whey protein are needed to define protein source to be used in nutritional interventions to protein synthesis, lean mass gain and oxidative stress in different populations. Keywords: Soybean Proteins. Milk Proteins. Protein Biosynthesis. Hypertrophy. ResumoO interesse do mercado de suplementação pelo consumo de proteína de soja para otimizar o desempenho físico e metabólico após o exercício está aumentando. No entanto, evidências sugerem que a ingestão da proteína de soja tem propriedades anabólicas mais baixas quando comparada à proteína do soro do leite. O objetivo desta revisão sistemática foi comparar os efeitos da suplementação com whey protein e proteína de soja na manutenção das funções musculares após o exercício. Esta revisão foi realizada usando os Itens de Relatório Preferidos para Revisões Sistemáticas e Meta-Análises (PRISMA). Os artigos foram pesquisados na base de dados Pubmed e incluíram estudos comparando os efeitos da proteína de soja e do consumo de proteínas do soro na síntese protéica, ganho de massa magra e redução do estresse oxidativo em resposta ao treinamento de resistência ou resistência. Treze ensaios foram incluídos nesta revisão. Os resultados mostraram que o consumo de proteína de soro é superior ao da proteína de soja em relação à síntese protéica e ao ganho de massa magra, mas a proteína de soja apresentou resultados superiores na redução do estresse oxidativo. Pesquisas futuras comparando a soja e a proteína do soro do leite são necessárias para definir a fonte protéica a ser usada em intervenções nutricionais para a síntese protéica, ganho de massa magra e estresse oxidativo em diferentes populações. Palavras-chave: Proteínas de Soja. Proteínas do Leite. Biossíntese de Proteínas. Hipertrofia.
A three year prospective study of functional and clinical outcomes of total hip replacement at a tertiary care hospital
Authors: Vijayanand Addanki , Jayasomeswar Narreddy 
Published: January 2020

<p class="abstract"><strong>Background:</strong> Hip joint is a crucial joint in the body whose function is essential for normal daily activities. Osteoarthritis of the hip cripples the daily functional capacity and total hip arthroplasty is considered to relieve the pain and increase the quality of life among these patients. The objective of present study was to assess the clinical and functional outcome in total hip replacement patients in terms of early joint function and stability of the hip joint and also to assess complications of procedures.</p><p class="abstract"><strong>Methods:</strong> A three-year prospective study was conducted on 58 cases that fulfilled the inclusion criteria. Ethical approval and consent were obtained for the study. Standard clinical and laboratory evaluation was performed on all the cases and the data was noted in Microsoft excel sheet and analyzed. Modified Harris hip scoring was done pre operatively and postoperatively and follow-up was done at discharge, 4<sup>th</sup> week, 6 months and one year.<strong></strong></p><p class="abstract"><strong>Results:</strong> Surgery was performed on 58 cases with age range 34-73 years and a mean of 43.58 years. Avascular necrosis was the main indication (55.2%). Maximum patient follow-up was 38 weeks. The mean preoperative score was 41.04 with minimum score being 10 and maximum being 76, postoperative score was 99.63 with minimum 64 and maximum was 109. Statistical significance was observed between preoperative and postoperative scores with regard to the parameters of pain, gait, functional activity and range of motion. Anterior thigh pain was the common postoperative complaint (15.5%).</p><p class="abstract"><strong>Conclusions:</strong> In conclusion, total hip replacement gives good clinical and functional outcomes. However, the outcomes are influenced by multiple factors which include indication for surgery, age of the cases, and type of prosthesis, operative technique and post-operative follow up.</p><p class="abstract"> </p>