bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of using a bioabsorbable implant (Latera) to treat nasal valve collapse in patients with nasal obstruction: systemic review and meta‐analysis
Authors: Kim Do Hyun , Lee Hyun Ho , Kim Sun Hong , Hwang Se Hwan 
Published: April 2020

BackgroundNasal obstruction is a common cause of breathing problems with lateral wall insufficiency (LWI) a key anatomic contributor. Recently, a bioabsorbable nasal implant was introduced to correct LWI and treat nasal obstruction. The goal of this study was to perform a systematic review with meta‐analysis to determine the efficacy of the bioabsorbable nasal implant for treating nasal obstruction caused by LWI.MethodsFive databases (PubMed, SCOPUS, EMBASE, Web of Science, and the Cochrane Database) were independently reviewed by 2 researchers, starting at the earliest time point recorded in the database to September 2019. Studies that scored endoscopic lateral wall movement and nasal obstruction related to quality of life (QOL) postoperatively before and after bioabsorbable nasal implants and those that compared the outcomes of nasal implants (treatment group) with outcomes of sham surgery (control group) were included in the analysis.ResultsFive studies (396 patients) met the inclusion criteria. Bioabsorbable nasal implants significantly reduced endoscopic lateral wall motion compared to pretreatment values and also improved QOL at 12 months postoperatively. Most adverse effects following the nasal implant, such as skin or mucosal reaction, infection, or implant retrieval, were reported with a 5% incidence rate. All adverse outcomes were resolved without significant sequelae. Compared with sham surgery, bioabsorbable nasal implants significantly improved disease‐specific QOL.ConclusionBioabsorbable nasal implants may reduce nasal wall movement and subjective symptom scores compared to preoperative status. More randomized clinical trials must be conducted to further verify the effectiveness of bioabsorbable nasal implants.
Monitoring H2O2on the Surface of Single Cells with Liquid Crystal Elastomer Microspheres
Authors: Li Weiwei , Khan Mashooq , Lin Ling , Zhang Qiang , Feng Shuo , Wu Zengnan , Lin Jin‐Ming 
Published: April 2020

AbstractLive‐imaging of signaling molecules released from living cells is a fundamental challenge in life sciences. Herein, we synthesized liquid crystal elastomer microspheres functionalized with horse‐radish peroxidase (LCEM‐HRP), which can be immobilized directly on the cell membrane to monitor real‐time release of H2O2at the single‐cell level. LCEM‐HRP could report H2O2through a concentric‐to‐radial (C‐R) transfiguration, which is due to the deprotonation of LCEM‐HRP and the break of inter or intra‐chain hydrogen bonding in LCEM‐HRP caused by HRP‐catalyzed reduction of H2O2. The level of transfiguration of LCEM‐HRP revealed the different amounts of H2O2released from cells. The estimated detection sensitivity was ≈2.2×10−7 μmfor 10 min of detection time. The cell lines and cell–cell heterogeneity was explored from different configurations. LCEM‐HRP presents a new approach for in situ real‐time imaging of H2O2release from living cells and can be the basis for seeking more advanced chemical probes for imaging of various signaling molecules in the cellular microenvironment.
Nucleic Acid Aptamers for Molecular Diagnostics and Therapeutics: Advances and Perspectives
Authors: Li Long , Xu Shujuan , Yan He , Li Xiaowei , Yazd Hoda Safari , Li Xiang , Huang Tong , Cui Cheng , Jiang Jianhui , Tan Weihong 
Published: April 2020

AbstractThe advent of SELEX (systematic evolution of ligands by exponential enrichment) technology has shown the ability to evolve artificial ligands with affinity and specificity able to meet growing clinical demand for probes that can, for example, distinguish between the target leukemia cells and other cancer cells within the matrix of heterogeneity, which characterizes cancer cells. Though antibodies are the conventional and ideal choice as a molecular recognition tool for many applications, aptamers complement the use of antibodies due to many unique advantages, such as small size, low cost, and facile chemical modification. This Minireview will focus on the novel applications of aptamers and SELEX, as well as opportunities to develop molecular tools able to meet future clinical needs in biomedicine.
Distinctive spring shortwave cloud radiative effect and its inter‐annual variation over southeastern China
Authors: Li Jiandong , You Qinglong , He Bian 
Published: April 2020

AbstractThe shortwave cloud radiative effect (SWCRE) plays a critical role in the earth's radiation balance, and its global mean magnitude is much larger than the warming effect induced by greenhouse gases. This study investigates the SWCRE at the top of the atmosphere and its inter‐annual variation over southeastern China (SEC) using satellite retrievals and ERA‐Interim reanalysis data. The results show that in this region the largest SWCRE with the maximum intensity up to −120 W·m−2 occurs in spring and is also the strongest between 60°S and 60°N. The domain‐averaged intensity of SWCRE is much larger than the longwave cloud radiative effect (LWCRE), suggesting the dominant cooling role of SWCRE in the regional atmosphere–surface system. The spring SWCRE over SEC shows a weak increasing trend and its anomalies in most years exceed those of LWCRE during 2000–2017. This means that SWCRE also plays a dominant role in the inter‐annual variation of regional cloud radiative effects. Over SEC, low‐ to mid‐level ascending motion and water vapor convergence during spring favor the generation and maintenance of cloud water, leading to strong SWCRE. Statistical analysis shows that the spatial pattern and intensity of the spring SWCRE are well correlated with the low‐ to mid‐level ascending motion and water vapor convergence. The temporal correlation coefficient between domain‐averaged spring SWCRE and 850–500‐hPa vertical velocity is .76 during 2000–2017. The long‐term variation in spring SWCRE over SEC can be inferred to some extent from regional ascending motion and associated large‐scale circulations.
Quantitative Gait Analysis in Duplication 15q Syndrome and Nonsyndromic ASD
Authors: Wilson Rujuta B. , Elashoff David , Gouelle Arnaud , Smith Beth A. , Wilson Andrew M. , Dickinson Abigail , Safari Tabitha , Hyde Carly , Jeste Shafali S. 
Published: April 2020

AbstractMotor impairments occur frequently in genetic syndromes highly penetrant for autism spectrum disorder (syndromic ASD) and in individuals with ASD without a genetic diagnosis (nonsyndromic ASD). In particular, abnormalities in gait in ASD have been linked to language delay, ASD severity, and likelihood of having a genetic disorder. Quantitative measures of motor function can improve our ability to evaluate motor differences in individuals with syndromic and nonsyndromic ASD with varying levels of intellectual disability and adaptive skills. To evaluate this methodology, we chose to use quantitative gait analysis to study duplication 15q syndrome (dup15q syndrome), a genetic disorder highly penetrant for motor delays, intellectual disability, and ASD. We evaluated quantitative gait variables in individuals with dup15q syndrome (n = 39) and nonsyndromic ASD (n = 21) and compared these data to a reference typically developing cohort. We found a gait pattern of slow pace, poor postural control, and large gait variability in dup15q syndrome. Our findings improve characterization of motor function in dup15q syndrome and nonsyndromic ASD. Quantitative gait analysis can be used as a translational method and can improve our identification of clinical endpoints to be used in treatment trials for these syndromes. Autism Res 2020, 13: 1102‐1110. © 2020 International Society for Autism Research, Wiley Periodicals, Inc.Lay SummaryMotor impairments, particularly abnormalities in walking, occur frequently in genetic syndromes highly penetrant for autism spectrum disorder (syndromic ASD). Here, using quantitative gait analysis, we find that individuals with duplication 15q syndrome have an atypical gait pattern that differentiates them from typically developing and nonsyndromic ASD individuals. Our findings improve motor characterization in dup15q syndrome and nonsyndromic ASD.
Differences in Developmental Functioning Profiles Between Male and Female Preschoolers Children With Autism Spectrum Disorder
Authors: Craig Francesco , Crippa Alessandro , De Giacomo Andrea , Ruggiero Marta , Rizzato Veronica , Lorenzo Alessandro , Fanizza Isabella , Margari Lucia , Trabacca Antonio 
Published: April 2020

AbstractThis study investigated differences in clinical symptoms and developmental functioning profiles as well as sex‐specific correlations of clinical characteristics and communication abilities, motor skills, and maladaptive behaviors in male and female preschoolers with autism spectrum disorder (ASD). Fifty‐two females (mean age 4.5 ± 2.16 years old) and 62 males (mean age 4.2 ± 1.17 years old) with ASD were enrolled and assessed by measures including the Autism Diagnostic Observation Schedule‐Second Edition (ADOS‐2) and Psychoeducational Profile‐Third Edition (PEP‐3). We found intellectual disability in 91.2% of the children. While preschoolers with ASD showed comparable severity of restricted and repetitive behaviors (P = 0.17), females with ASD were less severely affected than age and intelligence quotient‐matched males with ASD in the ADOS‐2 social affect domain (P value = 0.001) and calibrated severity scores (P = 0.002). Interestingly, sex‐specific linear regressions revealed that fine motor skills were predictive of impaired social affect in males but not in females. Specifically, motor skills might be the core feature for sex differences in ASD. Although preliminary, this finding suggests the need for more sex‐specific diagnostic and intervention strategies in order to improve early identification efforts and specific intervention targets.Lay SummaryLittle is known about differences in developmental and functional profiles in males and females with autism spectrum disorder (ASD). We found important similarities and differences in the core ASD symptoms between male and female preschoolers. In addition, fine motor skills seem to predict social affect impairment and ASD symptom severity in males with ASD. Autism Res 2020, 13: 1537–1547. © 2020 International Society for Autism Research, Wiley Periodicals, Inc.
Effect of nickel, cobalt, and iron on methanogenesis from methanol and cometabolic conversion of 1,2‐dichloroethene by Methanosarcina barkeri
Authors: Paulo Lara M. , Hidayat Mohamad R. , Moretti Giulio , Stams Alfons J. M. , Sousa Diana Z. 
Published: April 2020

AbstractMethanogens are responsible for the last step in anaerobic digestion (AD), in which methane (a biofuel) is produced. Some methanogens can cometabolize chlorinated pollutants, contributing for their removal during AD. Methanogenic cofactors involved in cometabolic reductive dechlorination, such as F430 and cobalamin, contain metal ions (nickel, cobalt, iron) in their structure. We hypothesized that the supplementation of trace metals could improve methane production and the cometabolic dechlorination of 1,2‐dichloroethene (DCE) by pure cultures of Methanosarcina barkeri. Nickel, cobalt, and iron were added to cultures of M. barkeri growing on methanol and methanol plus DCE. Metal amendment improved DCE dechlorination to vinyl chloride (VC): assays with 20 µM of Fe3+ showed the highest final concentration of VC (5× higher than in controls without Fe3+), but also in assays with 5.5 µM of Co2+ and 5 µM of Ni2+ VC formation was improved (3.5–4× higher than in controls without the respective metals). Dosing of metals could be useful to improve anaerobic removal of chlorinated compounds, and more importantly decrease the detrimental effect of DCE on methane production in anaerobic digesters.
Purification and properties of a laccase from the mushroom Agaricus sinodeliciosus
Authors: Hao Jingzhe , Zhang Weiwei , Wang Hexiang , Ziya Nur , Luo Ying , Jia Peisong , Zhang Guoqing , Ng Tzibun 
Published: April 2020

AbstractA homogeneous monomeric laccase (ASL) from Agaricus sinodeliciosus, with a molecular mass of 65 kDa, was isolated using ion‐exchange chromatography (CM‐cellulose and Q‐Sepharose) and gel‐filtration chromatography (Superdex 75). This laccase exhibited maximum activity at 50 °C and pH 5.0. Hg2+ and Cd2+ significantly inhibited its activity. The laccase displayed a Km value of 0.9 mM toward 2,2′‐azinobis‐(3‐ethylbenzthiazoline‐6‐sulfonate) (ABTS). In addition to ABTS, ASL exhibited higher affinity toward o‐toluidine and benzidine than other substrates. ASL is able to decolorize malachite green and Eriochrome black T.
Biosynthesis and characterization of poly(d‐lactate‐co‐glycolate‐co‐4‐hydroxybutyrate)
Authors: Choi So Young , Chae Tong Un , Shin Jihoon , Im Jung Ae , Lee Sang Yup 
Published: April 2020

AbstractPoly(d‐lactate‐co‐glycolate‐co‐4‐hydroxybutyrate) [poly(d‐LA‐co‐GA‐co‐4HB)] and poly(d‐lactate‐co‐glycolate‐co‐4‐hydroxybutyrate‐co‐d‐2‐hydroxybutyrate) [poly(d‐LA‐co‐GA‐co‐4HB‐co‐d‐2HB)] are of interest for their potential applications as new biomedical polymers. Here we report their enhanced production by metabolically engineered Escherichia coli. To examine the polymer properties, poly(d‐LA‐co‐GA‐co‐4HB) polymers having various monomer compositions (3.4–41.0mol% of 4HB) were produced by culturing the engineered E. coli strain expressing xylBC from Caulobacter crescentus, evolved phaC1 from Pseudomonas sp. MBEL 6‐19 (phaC1437), and evolved pct from Clostridium propionicum (pct540) in a medium supplemented with sodium 4HB at various concentrations. To produce these polymers without 4HB feeding, the 4HB biosynthetic pathway was additionally constructed by expressing Clostridium kluyveri sucD and 4hbD. The engineered E. coli expressing xylBC, phaC1437, pct540, sucD, and 4hbD successfully produced poly(d‐LA‐co‐GA‐co‐4HB‐co‐d‐2HB) and poly(d‐LA‐co‐GA‐co‐4HB) from glucose and xylose. Through modulating the expression levels of the heterologous genes and performing fed‐batch cultures, the polymer content and titer could be increased to 65.76wt% and 6.19g/L, respectively, while the monomer fractions in the polymers could be altered as desired. The polymers produced, in particular, the 4HB‐rich polymers showed viscous and sticky properties suggesting that they might be used as medical adhesives.
Using anticipated learning outcomes for backward design of a molecular cell biology Course‐based Undergraduate Research Experience
Authors: Hills Melissa , Harcombe Kimberley , Bernstein Nina 
Published: April 2020

AbstractAnticipated learning outcomes (LOs) were defined and used for the backward design of a Course‐based Undergraduate Research Experience (CURE). These LOs reflect the inquiry‐based nature of CUREs and capture key knowledge and skills inherent to scientific practice and essential in research. The LOs were used to plan a formative and summative assessment strategy to support and evaluate student achievement. A research question was identified that aligned with the learning goals of the course, provided an opportunity for discovery and iteration, and introduced a variety of molecular, cellular, and biochemical techniques. The course is offered to students in the final year of their degree and delivered over a 12‐week period with two 3‐hr labs each week. These LOs, and the rigorous assessment strategy used to support them, could be adapted to different projects. Likewise, the laboratory exercises are presented as a series of modules highlighting opportunities for adaptation to a variety of schedules.
Targeted double domain nanoplex based on galactosylated polyethylenimine enhanced the delivery of IL‐12 plasmid
Authors: Dehshahri Ali , Sadeghpour Hossein , Mohazzabieh Erfaneh , Saatchi Avval Sara , Mohammadinejad Reza 
Published: April 2020

AbstractThe objective of the present investigation was to design a targeted polyethylenimine (PEI)‐based polyplex by conjugating lactose bearing galactose groups on low molecular weight PEI (LMW PEI) grafted to a high molecular weight PEI (HMW PEI) via a succinic acid linker in order to restore the amine content of the whole conjugate used for ligand conjugation. The PEI conjugate was synthesized and characterized in terms of buffering capacity, particle size, zeta potential, plasmid condensation ability, and protection of DNA against degrading enzymes. Also, the transfection efficiency and cytotoxicity were evaluated in the cell line over‐expressing asialoglycoprotein receptors (ASGPRs) and compared with the cells lacking the receptors. The results demonstrated the ability of PEI conjugate in condensation of plasmid DNA and protection against enzyme degradation. The PEI conjugate formed nanoparticles of around 75 nm with higher buffering capacity compared with unmodified PEI. The polyplexes prepared by the modified PEI could increase the level of transgene up to four folds in the cells over‐expressing the receptor. The results demonstrated the separation of targeting and delivery domains could be considered as a strategy to restore the amine content of the PEI molecule utilized for targeting ligand conjugation.
Paleopathological study of a podal osteochondroma from the prehistoric Hypogeum of Calaforno (Sicily)
Authors: Varotto Elena , Militello Pietro Maria , Platania Erica , Sferrazza Paolo , Galassi Francesco Maria 
Published: April 2020

AbstractIn this article, we report a case of isolated podal osteochondroma from the prehistoric Hypogeum of Calaforno (Giarratana, Ragusa, Sicily). Although the phalanx exhibiting the benign tumoral mass comes from a context featuring several commingled remains, the very good state of preservation of this bone allowed us to perform a comprehensive study of the neoplasm by applying a multidisciplinary approach encompassing archeology, morphology, stereomicroscopy, and radiology. The results from this very ancient specimen have been assessed in the light of the available paleopathological literature and clinical implications currently encountered in modern patients.
HOXD9 promote epithelial‐mesenchymal transition and metastasis in colorectal carcinoma
Authors: Liu Mengwei , Xiao Yizhi , Tang Weimei , Li Jiaying , Hong Linjie , Dai Weiyu , Zhang Wenjing , Peng Ying , Wu Xiaosheng , Wang Jing , Chen Yaying , Bai Yang , Lin Jianjiao , Yang Qiong , Wang Yusi , Lin Zhizhao , Liu Side , Xiong Jing , Wang Jide , Xiang Li 
Published: April 2020

AbstractBackgroundHOXD9, a Hox family member, is involved in cancer growth and metastasis. But, its regulation mechanism at the molecular level particularly in colorectal cancer (CRC), is mostly unknown.MethodsThe HOXD9 protein expression levels were analyzed using immunofluorescence, immunohistochemistry (IHC) assays, and western blot. The in vivo and in vitro roles of HOXD9 in CRC were determined using colony formation and EdU incorporation, CCK‐8, wound scratch and transwell invasion assay, and animal models.ResultsExpression of HOXD9 was higher in CRC than in matched healthy tissues. High expression of HOXD9 has significantly associated with the American Joint Committee on Cancer (AJCC) stages, tumor differentiation, lymph node metastasis, and other serious invasions, and it had a poor prognosis. In vitro, HOXD9 encouraged proliferation, movement and EMT processes in cells of CRC. Also, TGF‐β1 promoted the expression of HOXD9 and this effect was dependent on the dose and downregulation of HOXD9 repressed TGF‐β1 ‐induced EMT. In vivo, HOXD9 promoted the invasive and metastasis of CRC cells via orthotopic implantation.ConclusionsThe ectopic expression of HOXD9 promoted the invasion metastasis in cells of the colorectal tumor by induction of EMT in vitro and vivo.
The value of renal score in both determining surgical strategies and predicting complications for renal cell carcinoma: A systematic review and meta‐analysis
Authors: Shi Naipeng , Zu Feng , Shan Yong , Chen Shuqiu , Xu Bin , Du Mulong , Chen Ming 
Published: April 2020

AbstractObjectivesRadical nephrectomy (RN) was the standard treatment for renal cell carcinoma (RCC). However, recent studies have found that partial nephrectomy (PN) could achieve similar effects as radical nephrectomy, and has the advantages of less bleeding and shorter hospital stay. The choice of surgical strategies has become a concern of clinicians, which could be guided by renal score introduced by Kutikov et al Therefore, we conducted this meta‐analysis to clarify the value of renal score of determining surgical strategies and predicting complications.MethodsThe keywords “RENAL score,” “renal nephrometry score,” or “nephrometry score” were used to retrieve electronic databases for relevant literature up to Feb 2020, including PubMed, Web of Science, and the Cochrane library. Surgical strategies and complications are outcome measures. Risk ratio (RR) with 95% confidence intervals (CI) is applied to assess the effect size.ResultsA total of 20 studies met the selection criteria for meta‐analysis. There was significant difference in RN operation rate for each subgroup (low‐moderate: RR = 3.50, 95% Cl = 2.60‐4.71, P < .001; low‐high: RR = 6.29, 95% Cl = 4.40‐9.00, P < .001; moderate‐high: RR = 1.80, 95% Cl = 1.39‐2.32, P < .001).The overall incidence of complications from high renal score group was significantly higher than that in low renal score group (low‐moderate: RR = 1.32, 95% Cl = 1.03‐1.69, P = .026; low‐high: RR = 2.45, 95% Cl = 1.48‐4.07, P = .001; moderate‐high: RR = 1.75, 95% Cl = 1.17‐2.61, P = .007).ConclusionsThis meta‐analysis indicated that renal score is an efficient tool for determining surgical strategies and predicting complications in PN. More prospective research is essential to verify the predictive value of renal score.