bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does practising hypnosis-derived communication techniques by oncology nurses translate into reduced pain and distress in their patients? An exploratory study
Authors: Ogez David , Aramideh Jennifer , Mizrahi Terry , Charest Marie-Claude , Plante Caroline , Duval Michel , Sultan Serge 
Published: June 2020

Objectives: To explore the effects of a hypnotic communication (HC) training for paediatric nurses in decreasing patients’ pain and distress during venipunctures. Methods: A 4-day theoretical and practical HC training was offered to five paediatric oncology nurses. The effects of HC were tested with 22 young cancer patients (13 girls, 9 boys, 10 ± 4 years) over four time points, with 88 encounters being video-recorded and coded in stable professional-patient dyads. Patients’ pain and distress were rated by patients and parents with visual analogue scales and coded from recordings using the Faces, Legs, Activity, Cry and Consolability (FLACC) scale. Results: We observed a significant decrease in pre-post distress reported by parents ( d = 0.45, p = 0.046). Two out of five nurses with higher skills acquisition had larger reduction in patients’ self-reported pain ( d = 1.03, p = 0.028), parents perceived pain ( d = 1.09, p = 0.042), distress ( d = 1.05, p = 0.043) as well as observed pain ( d = 1.22, p = 0.025). Favourable results on pain and distress did not maintain at follow-up. Conclusion and clinical implications: Training nurses in HC may translate into improved pain and distress in patients, both self-rated and observed provided that skills are used in practice. HC training is a promising non-pharmacological intervention to address pain in paediatrics.
Psycho-sensory relationships in chronic pain
Authors: Harvie Daniel S , Vasco Daniela , Sterling Michele , Low-Choy Samantha , Niederstrasser Nils G 
Published: June 2020

Background: Psychological variables contribute to pain- and injury-related outcomes. We examined the hypothesis that anatomical spread and intensity of persistent pain relate to anxiety-related variables: generalised anxiety, fear of pain and pain catastrophising. Methods: An online survey was used to gather data from 413 women with persistent pain (low back pain, n = 139; fibromyalgia syndrome, n = 95; neck pain, n = 55; whiplash, n = 41; rheumatoid arthritis, n = 37; migraine, n = 46). The spread and intensity of pain were assessed using the McGill pain chart and a Numerical Rating Scale. A Bayesian Structural Equation Model assessed if the intensity and spread of pain increased with anxiety-related variables. Men were also surveyed (n = 80), but the sample size was only sufficient for analysing if their data were consistent with the model for women. Results: Across subgroups of women, one standard deviation increase in catastrophising, generalised anxiety and fear corresponded to 27%, 7% and −1% additional pain areas and a 1.1, 0 and –0.1 change in pain intensity (on 0–10 scale), respectively. Overall, our clinical significance criterion – a 30% shift in pain variable in relation to one standard deviation increase in psychological variable – was not met. However, in subgroups it was met for pain spread (low back pain, neck pain and migraine) and pain intensity (migraine and neck pain) in relation to pain catastrophising. The model generally had low goodness-of-fit to men. Conclusion: These data support a meaningful relationship between some anxiety-related variables and pain in women for some conditions. Since the model did not consistently fit the men, we may conclude that the relationships are moderated by sex. Clinician attention to psychological variables as potential contributing factors can be justified; however, research is needed to understand the relationship and whether psychological treatment can reduce pain.
Development of vaccines against the sexually transmitted infections gonorrhoea, syphilis, Chlamydia, herpes simplex virus, human immunodeficiency virus and Zika virus
Authors: McIntosh Edwin David G. 
Published: June 2020

The success in preventing hepatitis B virus and human papillomavirus infections by means of vaccination paves the way for the development of other vaccines to prevent sexually transmitted infections (STIs) such as gonorrhoea, syphilis, chlamydia, herpes simplex virus, human immunodeficiency virus and Zika virus. The current status of vaccine development for these infections will be explored in this review. The general principles for success include the need for prevention of latency, persistence and repeat infections. A reduction in transmission of STIs would reduce the global burden of disease. Therapeutic activity of vaccines against STIs would be advantageous over preventative activity alone, and prevention of congenital and neonatal infections would be an added benefit. There would be an added value in the prevention of long-term consequences of STIs. It may be possible to re-purpose ‘old’ vaccines for new indications. One of the major challenges is the determination of the target populations for STI vaccination.
Prenatal Diagnosis of Placental Invasion: A Systematic Review and Meta-analysis on Accuracy of Ultrasonography and MRI in Diagnosis of Placental Invasion
Authors: Dahmarde Hamid , Parooie Fateme , Salarzaei Morteza 
Published: June 2020

Objective: The aim of this systematic review and meta-analysis was to evaluate and compare the diagnostic accuracy of ultrasonography (US) and magnetic resonance imaging (MRI) in the diagnosis of placental invasion. Method: Two independent scientists carried out a series of searches to obtain relevant research, released from January 1, 2009, to December 31, 2018. National databases (Magiran and SID), KoreaMed, and LILACS were reviewed for literature, released in other languages. A series of MeSH keywords was employed as part of the searching strategy (e.g., pregnancy, pregnant, placental invasion, accreta). A forest plot was built with Meta-DiSc, version 1.4. Results: For placental invasion, the sensitivity and specificity of MRI were 89% (95% confidence interval [CI], 83%–93% with a 95% confidence interval) and 97% (95% CI, 93%–99%), respectively. The sensitivity and specificity of US were 73% (95% CI, 66%–79%) and 89% (95% CI, 84%–93%), respectively. In the detection of placenta accreta solely (as the first grade of placental invasion), the sensitivity and specificity of MRI were 89.3% (95% CI, 86%–92%) and 84% (95% CI, 77%–89.6%). The sensitivity and specificity of US detection were 95.4% (95% CI, 93.8%–96.6%) and 87.3% (95% CI, 81.4%–91.9%), respectively. Conclusion: Due to the increased cesarean sections, placental invasion may be increasing. The diagnosis of prenatal placental invasion is important to reduce maternal morbidity and mortality. Recent trends indicate an increased use of MRI to evaluate placental invasion as a complement to US. The most accurate imaging findings are using US for placental lacunae and MRI to detect the dark intraplacental band.
Evaluation of the Diagnostic Value of Bedside Transthoracic Ultrasonography (TTUS) and Lower Extremity Three-Points Compression Duplex in the Diagnosis of the Pulmonary Embolism
Authors: Golshani Keihan , Sharafsaleh Mohammad 
Published: June 2020

Background: The present study aimed at evaluating the diagnostic value of lung transthoracic ultrasonography (TTUS) and lower extremity sonography versus computed tomography pulmonary angiography (CTPA) in the diagnosis of pulmonary embolus (PE). Materials and Methods: This study was performed on 104 patients with clinically suspected PE. CTPA and D-dimer was performed on all patients. Wells’ deep vein thrombosis criteria (WDVTC) was evaluated and recorded at the patient’s admission. Finally, the results of adding venous and lung sonography scores to the WDVTC with and without the results of D-dimer test were analyzed for predicting the diagnosis of PE. Results: There were 104 patients clinically suspected of having a PE and enrolled in the study. A PE was confirmed in 37.5% of this cohort. WDVTC had a sensitivity and specificity of 94.87% and 80.00%, respectively, to predict PE. When adding D-dimer to the WDVTC for patients unlikely to have a PE (WDVTC≤4), the sensitivity and specificity of positive D-dimer in diagnosis of PE were 94.87% and 56.92%, respectively ( P < .001). Conclusion: According to these study results, the modification of the WDVTC along with the results of lung TTUS and lower extremity venous sonogram significantly increase the diagnostic power for PE.
Plasmodium falciparum sexual parasites develop in human erythroblasts and affect erythropoiesis
Authors: Neveu Gaëlle , Richard Cyrielle , Dupuy Florian , Behera Prativa , Volpe Fiona , Subramani Pradeep Annamalai , Marcel-Zerrougui Benjamin , Vallin Patrice , Andrieu Muriel , Minz Aruna Mukti , Azar Nabih , Martins Rafael M. , Lorthiois Audrey , Gazeau Florence , Lopez-Rubio José-Juan , Mazier Dominique , Silva Amanda K. A. , Satpathi Sanghamitra , Wassmer Samuel C. , Verdier Frédérique , Lavazec Catherine 
Published: June 2020

AbstractPlasmodium falciparum gametocytes, the sexual stage responsible for malaria parasite transmission from humans to mosquitoes, are key targets for malaria elimination. Immature gametocytes develop in the human bone marrow parenchyma, where they accumulate around erythroblastic islands. Notably though, the interactions between gametocytes and this hematopoietic niche have not been investigated. Here, we identify late erythroblasts as a new host cell for P falciparum sexual stages and show that gametocytes can fully develop inside these nucleated cells in vitro and in vivo, leading to infectious mature gametocytes within reticulocytes. Strikingly, we found that infection of erythroblasts by gametocytes and parasite-derived extracellular vesicles delay erythroid differentiation, thereby allowing gametocyte maturation to coincide with the release of their host cell from the bone marrow. Taken together, our findings highlight new mechanisms that are pivotal for the maintenance of immature gametocytes in the bone marrow and provide further insights on how Plasmodium parasites interfere with erythropoiesis and contribute to anemia in malaria patients.
Synergistic targeting of FLT3 mutations in AML via combined menin-MLL and FLT3 inhibition
Authors: Dzama Margarita M. , Steiner Marlene , Rausch Johanna , Sasca Daniel , Schönfeld Jonas , Kunz Kerstin , Taubert Martha C. , McGeehan Gerard M. , Chen Chun-Wei , Mupo Annalisa , Hähnel Patricia , Theobald Matthias , Kindler Thomas , Koche Richard P. , Vassiliou George S. , Armstrong Scott A. , Kühn Michael W. M. 
Published: June 2020

Abstract
The interaction of menin (MEN1) and MLL (MLL1, KMT2A) is a dependency and provides a potential opportunity for treatment of NPM1-mutant (NPM1mut) and MLL-rearranged (MLL-r) leukemias. Concomitant activating driver mutations in the gene encoding the tyrosine kinase FLT3 occur in both leukemias and are particularly common in the NPM1mut subtype. In this study, transcriptional profiling after pharmacological inhibition of the menin-MLL complex revealed specific changes in gene expression, with downregulation of the MEIS1 transcription factor and its transcriptional target gene FLT3 being the most pronounced. Combining menin-MLL inhibition with specific small-molecule kinase inhibitors of FLT3 phosphorylation resulted in a significantly superior reduction of phosphorylated FLT3 and transcriptional suppression of genes downstream of FLT3 signaling. The drug combination induced synergistic inhibition of proliferation, as well as enhanced apoptosis, compared with single-drug treatment in models of human and murine NPM1mut and MLL-r leukemias harboring an FLT3 mutation. Primary acute myeloid leukemia (AML) cells harvested from patients with NPM1mutFLT3mut AML showed significantly better responses to combined menin and FLT3 inhibition than to single-drug or vehicle control treatment, whereas AML cells with wild-type NPM1, MLL, and FLT3 were not affected by either of the 2 drugs. In vivo treatment of leukemic animals with MLL-r FLT3mut leukemia reduced leukemia burden significantly and prolonged survival compared with results in the single-drug and vehicle control groups. Our data suggest that combined menin-MLL and FLT3 inhibition represents a novel and promising therapeutic strategy for patients with NPM1mut or MLL-r leukemia and concurrent FLT3 mutation.
CRISPR-based screens uncover determinants of immunotherapy response in multiple myeloma
Authors: Ramkumar Poornima , Abarientos Anthony B. , Tian Ruilin , Seyler Meghan , Leong Jaime T. , Chen Merissa , Choudhry Priya , Hechler Torsten , Shah Nina , Wong Sandy W. , Martin Thomas G. , Wolf Jeffrey L. , Roybal Kole T. , Pahl Andreas , Taunton Jack , Wiita Arun P. , Kampmann Martin 
Published: June 2020

AbstractCancer cells commonly develop resistance to immunotherapy by loss of antigen expression. Combinatorial treatments that increase levels of the target antigen on the surface of cancer cells have the potential to restore efficacy to immunotherapy. Here, we use our CRISPR interference– and CRISPR activation–based functional genomics platform to systematically identify pathways controlling cell surface expression of the multiple myeloma immunotherapy antigen B-cell maturation antigen (BCMA). We discovered that pharmacologic inhibition of HDAC7 and the Sec61 complex increased cell surface BCMA, including in primary patient cells. Pharmacologic Sec61 inhibition enhanced the antimyeloma efficacy of a BCMA-targeted antibody-drug conjugate. A CRISPR interference chimeric antigen receptor T cells (CAR-T cells) coculture screen enabled us to identify both antigen-dependent and antigen-independent mechanisms controlling response of myeloma cells to BCMA-targeted CAR-T cells. Thus, our study shows the potential of CRISPR screens to uncover mechanisms controlling response of cancer cells to immunotherapy and to suggest potential combination therapies.
Venetoclax and hypomethylating agents (HMAs) induce high response rates in MDS, including patients after HMA therapy failure
Authors: Ball Brian J. , Famulare Christopher A. , Stein Eytan M. , Tallman Martin S. , Derkach Andriy , Roshal Mikhail , Gill Saar I. , Manning Benjamin M. , Koprivnikar Jamie , McCloskey James , Testi Rebecca , Prebet Thomas , Al Ali Najla H. , Padron Eric , Sallman David A. , Komrokji Rami S. , Goldberg Aaron D. 
Published: June 2020

Key Points
Treatment-naive and relapsed/refractory MDS patients receiving venetoclax and HMAs have an ORR of 59% with 63% of responders proceeding to transplant. Allogeneic stem cell transplantation after treatment with venetoclax in combination with HMA is associated with prolonged survival.
Genetic and evolutionary patterns of treatment resistance in relapsed B-cell lymphoma
Authors: Rushton Christopher K. , Arthur Sarah E. , Alcaide Miguel , Cheung Matthew , Jiang Aixiang , Coyle Krysta M. , Cleary Kirstie L. S. , Thomas Nicole , Hilton Laura K. , Michaud Neil , Daigle Scott , Davidson Jordan , Bushell Kevin , Yu Stephen , Rys Ryan N. , Jain Michael , Shepherd Lois , Marra Marco A. , Kuruvilla John , Crump Michael , Mann Koren , Assouline Sarit , Connors Joseph M. , Steidl Christian , Cragg Mark S. , Scott David W. , Johnson Nathalie A. , Morin Ryan D. 
Published: June 2020

Abstract
Diffuse large B-cell lymphoma (DLBCL) patients are typically treated with immunochemotherapy containing rituximab (rituximab, cyclophosphamide, hydroxydaunorubicin-vincristine (Oncovin), and prednisone [R-CHOP]); however, prognosis is extremely poor if R-CHOP fails. To identify genetic mechanisms contributing to primary or acquired R-CHOP resistance, we performed target-panel sequencing of 135 relapsed/refractory DLBCLs (rrDLBCLs), primarily comprising circulating tumor DNA from patients on clinical trials. Comparison with a metacohort of 1670 diagnostic DLBCLs identified 6 genes significantly enriched for mutations upon relapse. TP53 and KMT2D were mutated in the majority of rrDLBCLs, and these mutations remained clonally persistent throughout treatment in paired diagnostic-relapse samples, suggesting a role in primary treatment resistance. Nonsense and missense mutations affecting MS4A1, which encodes CD20, are exceedingly rare in diagnostic samples but show recurrent patterns of clonal expansion following rituximab-based therapy. MS4A1 missense mutations within the transmembrane domains lead to loss of CD20 in vitro, and patient tumors harboring these mutations lacked CD20 protein expression. In a time series from a patient treated with multiple rounds of therapy, tumor heterogeneity and minor MS4A1-harboring subclones contributed to rapid disease recurrence, with MS4A1 mutations as founding events for these subclones. TP53 and KMT2D mutation status, in combination with other prognostic factors, may be used to identify high-risk patients prior to R-CHOP for posttreatment monitoring. Using liquid biopsies, we show the potential to identify tumors with loss of CD20 surface expression stemming from MS4A1 mutations. Implementation of noninvasive assays to detect such features of acquired treatment resistance may allow timely transition to more effective treatment regimens.
Severe low cerebral oximetry in difficult cardiopulmonary bypass weaning of low body-weight infant: a case report and literature review
Authors: Hao Xuechao , Wei Wei 
Published: June 2020

Abstract
Background
For infants undergoing complex cardiac surgery, hemodynamic management after cardiopulmonary bypass (CPB) is challenging because of severe myocardial edema, vasomotor dysfunction and weak tolerance to a change in blood volume. More importantly, the lack of availability of equipment for advanced monitoring, such as transesophageal echocardiography or transthoracic echocardiography, restricts the accurate assessment of hemodynamics.

Case presentation
This is a case of severe hypotension and non-detectable pulse oxygen saturation (SpO2) after CPB in a low-weight infant who had normal blood pressure and oxygen saturation before surgery. Epinephrine and milrinone were administered with cerebral oximetry monitoring rather than blood pressure measurements because cerebral oximetry was more responsive to treatment than blood pressure. Under the guidance of cerebral oximetry, the infant was successfully weaned from CPB and recovered after surgery without adverse neurological events.

Conclusions
For infants who develop refractory hypotension and failure in SpO2 monitoring during the CPB weaning period, cerebral oximetry provides an index for assessing brain perfusion and valuable guidance for appropriate inotropic treatment.
Accuracy and trending abilities of finger plethysmographic blood pressure and cardiac output compared to invasive measurements during caesarean delivery in healthy women: an observational study
Authors: Omenås Ivar N. , Tronstad Christian , Rosseland Leiv Arne 
Published: June 2020

Abstract
Background
In women presenting for caesarean section under spinal anesthesia, continuous measurement of circulatory aspects, such as blood pressure and cardiac output, is often needed. At present, invasive techniques are used almost exclusively. Reliable non-invasive monitoring would be welcome, as it could be safer, less uncomfortable, and quick and easy to apply. We aimed to evaluate whether a non-invasive, finger plethysmographic device, the ccNexFin monitor, can replace invasively measured blood pressure in the radial artery, and whether cardiac output measurements from this device can be used interchangeably with measurements from the mini-invasive LiDCO monitor currently in use at our institution.

Methods
Simultaneous invasive measurements were compared to ccNexFin in 23 healthy women during elective caesarean section under spinal anesthesia. We used Bland Altman statistics to assess agreement, and polar plot methodology to judge trending abilities with pre-defined limits.

Results
Mean arterial and systolic pressures showed biases (invasive – ccNexFin) of − 4.3 and 12.2 mmHg, with limits of agreement of − 15.9 – 7.4 and − 11.1 – 35.6, respectively. The ccNexFin trending abilities were within the suggested limits for mean pressure but insufficient for systolic pressure compared to invasive measurements. Cardiac output had a small bias of 0.2 L/min, but wide limits of agreement of − 2.6 – 3.0. The ccNexFin trending abilities compared to the invasive estimated values (LiDCO) were unsatisfactory.

Conclusions
We consider the ccNexFin monitor to have sufficient accuracy in measuring mean arterial pressure. The limits of agreement for systolic measurements were wider, and the trending ability compared to invasive measurements was outside the recommended limit. The ccNexFin is not reliable for cardiac output measurements or trend in pregnant women for caesarean delivery under spinal anesthesia.

Trial registration
Registered May 23, 2013, at ClinicalTrials.gov under number NCT01861132.
Combined sodium Dimercaptopropanesulfonate and zinc versus D-penicillamine as first-line therapy for neurological Wilson’s disease
Authors: Zhang Jing , Xiao Lulu , Yang Wenming 
Published: June 2020

Abstract
Background
Even though recent research has achieved significant advancement in the development of therapeutic approaches for Wilson’s diseases (WD), the current treatment options available for WD are still limited, especially for WD patients with neurological symptoms. This study is intended to compare the therapeutic approaches for WD patients with neurological symptoms receiving either combined sodium 2, 3-dimercapto-1-propane sulfonate (DMPS) and zinc treatment or D-penicillamine (DPA) monotherapy as first-line therapy, and identify the more effective therapeutic approach.

Methods
The case records of 158 patients diagnosed with neurological WD were retrospectively analyzed. These patients treated with intravenous DMPS + Zinc and in combination with oral zinc as a maintenance therapy (Group 1) or DPA alone (Group 2) for 1 year. During the period of treatment, the neurological symptoms of the patients were assessed using the Global Assessment Scale (GAS) and Barthel index. The key hematological and biochemical parameters of the patients (such as the levels of aminotransferase, serum ceruloplasmin, 24-h urine copper excretion), as well as adverse effects were recorded and analyzed.

Results
Ninety-three patients in Group 1, displayed decreased GAS scores and increased Barthel indexes consistently in comparison with the baseline (P < 0.01). Among them, 82 patients (88.2%) exhibited significant neurological improvement after 1 year, while 8 patients (8.6%) experienced neurological deterioration. Among the 65 patients in Group 2, 37 patients (58.5%) exhibited neurological improvements, while 17 patients (26.2%) experienced neurological deterioration after 1-year follow up. Six patients discontinued their treatment midway due to their exacerbating neurological symptoms. A comprehensive comparison of the effectiveness of the two courses of treatment revealed that patients in group 1 demonstrated a higher improvement ratio (P < 0.01) and lower worsening ratio of the neurological symptoms for the patients (P < 0.01) in comparison to the patients in group 2. Meanwhile, renal function, liver enzyme and blood cell counts remained stabilized in group1.

Conclusions
This study indicates that the combined therapeutic approach of DPMS and zinc may be a preferred first-line therapy in treating the neurological symptoms of WD, in comparison to the treatment with DPA.