bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circulation of Tick-Borne Spirochetes in Tick and Small Mammal Communities in Santa Barbara County, California, USA
Authors: MacDonald Andrew J , Weinstein Sara B , O’Connor Kerry E , Swei Andrea 
Published: January 2020

Abstract
A diversity of Borrelia burgdorferi sensu lato (Johnson, Schmid, Hyde, Steigerwalt & Brenner) (Spirochaetales: Spirochaetaceae) genomospecies, including the Lyme disease agent, Borrelia burgdorferi sensu stricto (s.s.), have been identified in the western United States. However, enzootic transmission of B. burgdorferi s.l. in small mammals and ticks is poorly characterized throughout much of the region. Here we report prevalence of B. burgdorferi s.l. in small mammal and tick communities in the understudied region of southern California. We found B. burgdorferi s.l. in 1.5% of Ixodes species ticks and 3.6% of small mammals. Infection was uncommon (~0.3%) in Ixodes pacificus Cooley and Kohls (Acari: Ixodidae), the primary vector of the Lyme disease agent to humans in western North America, but a diversity of spirochetes—including Borrelia bissettiae, Borrelia californiensis, Borrelia americana, and B. burgdorferi s.s.—were identified circulating in Ixodes species ticks and their small mammal hosts. Infection with B. burgdorferi s.l. is more common in coastal habitats, where a greater diversity of Ixodes species ticks are found feeding on small mammal hosts (four species when compared with only I. pacificus in other sampled habitats). This provides some preliminary evidence that in southern California, wetter coastal areas might be more favorable for enzootic transmission than hotter and drier climates. Infection patterns confirm that human transmission risk of B. burgdorferi s.s. is low in this region. However, given evidence for local maintenance of B. burgdorferi s.l., more studies of enzootic transmission may be warranted, particularly in understudied regions where the tick vector of B. burgdorferi s.s. occurs.
Photometric characterization of multiple populations in star clusters: the impact of the first dredge-up
Authors: Salaris Maurizio , Usher Chris , Martocchia Silvia , Dalessandro Emanuele , Bastian Nate , Saracino Sara , Cassisi Santi , Cabrera-Ziri Ivan , Lardo Carmela 
Published: January 2020

ABSTRACT
The existence of star-to-star light-element abundance variations (multiple populations, MPs) in massive Galactic and extragalactic star clusters older than about 2 Gyr is by now well established. Photometry of red giant branch (RGB) stars has been and still is instrumental in enabling the detection and characterization of cluster MPs, through the appropriate choices of filters, colours, and colour combinations that are mainly sensitive to N and – to a lesser degree – C stellar surface abundances. An important issue not yet properly addressed is that the translation of the observed widths of the cluster RGBs to abundance spreads must account for the effect of the first dredge-up on the surface chemical patterns, hence on the spectral energy distributions of stars belonging to the various MPs. We have filled this gap by studying theoretically the impact of the dredge-up on the predicted widths of RGBs in clusters hosting MPs. We find that for a given initial range of N abundances, the first dredge-up reduces the predicted RGB widths in N-sensitive filters compared to the case when its effect on the stellar spectral energy distributions is not accounted for. This reduction is a strong function of age and has also a dependence on metallicity. The net effect is an underestimate of the initial N-abundance ranges from RGB photometry if the first dredge-up is not accounted for in the modelling, and also the potential determination of spurious trends of N-abundance spreads with age.
Pulsation mode switching in the pulsating star HD 81589 and a proposed classification as a field FaRPB star
Authors: van Heerden P , Engelbrecht C A , Martinez P 
Published: January 2020

ABSTRACT
Extensive multicolour photometry and low-resolution spectroscopy obtained for HD 81589 place the star in the gap between δ Scuti and slowly pulsating B pulsators on the main sequence. A clear episode of pulsation mode switching has apparently occurred in HD 81589, with its primary pulsation frequency switching from 4.57 to 3.71 c d−1 in less than 6 months. The pulsation frequencies obtained for this star match remarkably well with modelled frequencies of prograde sectoral g-modes for rapidly rotating late B stars on the main sequence. This match, coupled with the rapid rotation rate determined for HD 81589, implies that HD 81589 is one of the first identified fast-rotating pulsating B-type (FaRPB) stars found in the field, complementing the sample of FaRPB stars previously discovered in the open cluster NGC 3766.
The Genomes of the Livebearing Fish Species Poeciliopsis retropinna and Poeciliopsis turrubarensis Reflect Their Different Reproductive Strategies
Authors: van Kruistum Henri , Guernsey Michael W , Baker Julie C , Kloet Susan L , Groenen Martien A M , Pollux Bart J A , Megens Hendrik-Jan 
Published: January 2020

AbstractThe evolution of a placenta is predicted to be accompanied by rapid evolution of genes involved in processes that regulate mother–offspring interactions during pregnancy, such as placenta formation, embryonic development, and nutrient transfer to offspring. However, these predictions have only been tested in mammalian species, where only a single instance of placenta evolution has occurred. In this light, the genus Poeciliopsis is a particularly interesting model for placenta evolution, because in this genus a placenta has evolved independently from the mammalian placenta. Here, we present and compare genome assemblies of two species of the livebearing fish genus Poeciliopsis (family Poeciliidae) that differ in their reproductive strategy: Poeciliopsis retropinna which has a well-developed complex placenta and P. turrubarensis which lacks a placenta. We applied different assembly strategies for each species: PacBio sequencing for P. retropinna (622-Mb assembly, scaffold N50 of 21.6 Mb) and 10× Genomics Chromium technology for P. turrubarensis (597-Mb assembly, scaffold N50 of 4.2 Mb). Using the high contiguity of these genome assemblies and near-completeness of gene annotations to our advantage, we searched for gene duplications and performed a genome-wide scan for genes evolving under positive selection. We find rapid evolution in major parts of several molecular pathways involved in parent–offspring interaction in P. retropinna, both in the form of gene duplications as well as positive selection. We conclude that the evolution of the placenta in the genus Poeciliopsis is accompanied by rapid evolution of genes involved in similar genomic pathways as found in mammals.
An internal deletion of ADAR rescued by MAVS deficiency leads to a minute phenotype
Authors: Bajad Prajakta , Ebner Florian , Amman Fabian , Szabó Brigitta , Kapoor Utkarsh , Manjali Greeshma , Hildebrandt Alwine , Janisiw Michael P , Jantsch Michael F 
Published: January 2020

Abstract
The RNA-editing protein ADAR is essential for early development in the mouse. Genetic evidence suggests that A to I editing marks endogenous RNAs as ‘self’. Today, different Adar knockout alleles have been generated that show a common phenotype of apoptosis, liver disintegration, elevated immune response and lethality at E12.5. All the Adar knockout alleles can be rescued by a concomitant deletion of the innate immunity genes Mavs or Ifih1 (MDA5), albeit to different extents. This suggests multiple functions of ADAR. We analyze AdarΔ7-9 mice that show a unique growth defect phenotype when rescued by Mavs. We show that AdarΔ7-9 can form a truncated, unstable, editing deficient protein that is mislocalized. Histological and hematologic analysis of these mice indicate multiple tissue- and hematopoietic defects. Gene expression profiling shows dysregulation of Rps3a1 and Rps3a3 in rescued AdarΔ7-9. Consistently, a distortion in 40S and 60S ribosome ratios is observed in liver cells. This dysregulation is also seen in AdarΔ2-13; Mavs−/− but not in AdarE861A/E861A; Ifih1−/− mice, suggesting editing-independent functions of ADAR in regulating expression levels of Rps3a1 and Rps3a3. In conclusion, our study demonstrates the importance of ADAR in post-natal development which cannot be compensated by ADARB1.
An antenatal alcohol service evaluation of the north-east of England and north Cumbria
Authors: Howlett Helen 
Published: January 2020

AbstractBackgroundNHS England’s ‘Better Births’ strategy aims to improve maternal and infant health outcomes. A strategic priority identified in the north-east local maternity system is to reduce alcohol consumption in pregnancy due to the documented diverse risks of harm to mother and baby, including foetal alcohol spectrum disorder.AimsTo evaluate current alcohol prevention, screening and treatment service provision in maternity care across the region, and inform future recommendations.MethodsA service evaluation survey was developed to systematically consult strategic stakeholders across all nine maternity trusts in the region over a 2-month period in 2018. Content analysis was employed to identify fundamental themes and inform recommendations for practice.ResultsHigh variation was reported throughout regional clinical practices, service provision and staff training. For example, a number of alcohol screening tools were identified, each with diverse thresholds for referral; reported data collection and documentation practices were multifarious, incomparable and unquantifiable; audit was rare and guidelines were primarily influenced by local commissioning agreements.DiscussionStandardized patient pathways involving alcohol screening and management practices are required, and sharing best practices will facilitate referrals and support regardless of location. The implementation of these recommendations requires appropriate leadership, commissioning and training strategies.
Clinical significance of radiological patterns of HRCT and their association with macrophage activation in dermatomyositis
Authors: Zuo Yu , Ye Lifang , Liu Min , Li Shanshan , Liu Weifang , Chen Fang , Lu Xin , Gordon Patrick , Wang Guochun , Shu Xiaoming 
Published: January 2020

Abstract

Objectives
To evaluate the distribution of radiological characteristics stratified by different myositis-specific autoantibodies, identify prognostic value of high-resolution CT (HRCT) patterns in DM-associated interstitial lung disease (DM-ILD), and explore the possible mechanism associated with macrophage activation.

Methods
We enrolled 165 patients with PM/DM-ILD. The distribution of HRCT radiological types with different myositis-specific autoantibodies and the relationship between radiological features and ILD course and prognosis were analysed. Additionally, the potential role of macrophage activation in rapidly progressive ILD (RP-ILD) with DM was studied.

Results
The organizing pneumonia pattern was dominant in HRCT findings of patients with DM-ILD, especially those with anti-SAE (6/6, 100%) and anti-MDA5 (46/62, 74.2%) antibodies. The ratios of organizing pneumonia and nonspecific interstitial pneumonia patterns were almost equal in patients with aminoacyl tRNA synthetase antibodies, and nonspecific interstitial pneumonia pattern was associated with a mild clinical course. Lower lung zone consolidation in HRCT was related to RP-ILD in both anti-MDA5 and anti-aminoacyl tRNA synthetase antibody-positive groups. Ferritin levels of &gt;1000 ng/ml (odds ratio (OR), 12.3; P=0.009), elevated carcinoembryonic antigen (OR, 5.8; P=0.046) and carbohydrate antigen 19–9 (OR, 7.8; P=0.018) were independent predictors of a lower lung zone consolidation pattern in anti-MDA5 antibody-positive DM. The infiltration of CD163-positive macrophages into alveolar spaces was significantly higher in the DM-RP-ILD group than in the chronic DM-ILD group.

Conclusion
HRCT patterns are different among variable myositis-specific autoantibodies positive patients with ILD and lower zone consolidation in HRCT correlated with RP-ILD in DM. Activated macrophages may contribute to the pathogenesis of RP-ILD in DM.
COMPLIANCE TESTING FOR HUMAN BODY MODEL EXPOSURE TO ELECTROMAGNETIC FIELDS FROM A HIGH-POWER WIRELESS CHARGING SYSTEM FOR DRONES
Authors: Ahn Jangyong , Hong Seon-Eui , Kim Haerim , Chun Yangbae , Choi Hyung-Do , Kim Kibeom , Andrés Brito , Choi Junsung , Ahn Seungyoung 
Published: January 2020

AbstractRecently, a wireless charging system (WCS) for drones has been extensively studied, although standards for compliance testing of a WCS for drones have yet to be established. In this study, we propose methods for human exposure assessments of a WCS for drones by comprehensively considering the various positions of the system and the postures of human body models. The electromagnetic fields from a WCS are modeled and the internal quantities of the human body models, consisting of current density, internal electric field and specific absorption rate, are calculated. The incident fields around the WCS and the internal quantities are analyzed at 140 kHz, which is the operating frequency of the WCS applied. Results of an exposure assessment based on the confirmed worst-case scenario are presented. In addition, the internal quantities depending on the human body models and the material characteristics of the simplified models are also discussed using four different anatomical and simplified human body models.
New room to maneuver? National tax policy under increasing financial transparency
Authors: Ahrens Leo , Bothner Fabio , Hakelberg Lukas , Rixen Thomas 
Published: January 2020

Abstract
Why have Organisation for Economic Co-operation and Development (OECD) governments raised taxes on dividends at the shareholder level since 2008? Previous research points to the importance of budget deficits and voter demand for compensatory fairness in the aftermath of the financial crisis. We complement this literature by showing that the effect of domestic drivers of tax increases on capital income crucially depends on the level of financial transparency in a country’s investment network. Low financial transparency increases the risk of capital flight in response to a tax hike, whereas high financial transparency reduces this risk. Hence, governments facing fiscal pressure become more likely to raise taxes on capital income when transparency is high. To substantiate our argument, we construct an original indicator of financial transparency in countries’ investment networks, which we utilize in a regression analysis of tax reforms by 204 cabinets in 35 OECD countries between 2001 and 2018.
Time to First Cigarette: A Potential Clinical Screening Tool for Nicotine Dependence
Authors: Branstetter Steven A. , Muscat Joshua E. , Mercincavage Melissa 
Published: January 2020

Objectives:
The time to first cigarette (TTFC) of the day has been identified as the best single-item indicator of nicotine dependence. However, TTFC has not been extensively used in clinical settings and is not a criterion for tobacco use disorder, perhaps due to a lack of information about TTFC's predictive value. This review provides a synthesis of the accumulating literature on TTFC's relationships with nicotine dependence, identify gaps, and inform future clinical and epidemiologic research of potential uses of TTFC.

Methods:
A systematic review of the literature was conducted using PubMed, Google Scholar, and Web of Science ISI databases. We identified 16 articles examining the relation between TTFC and negative outcomes associated with high levels of nicotine addiction and Tobacco Use Disorders (eg, high levels of nicotine and toxicant exposure, progressive use over time, failed cessation, head-and-neck cancers).

Results:
Earlier TTFC was consistently associated with greater likelihood of cessation failure and relapse, and higher levels of biomarkers of tobacco exposure. Several of these associations were found among both adult and adolescent smokers, and remained even after accounting for smoking behaviors (eg, cigarettes/day).

Conclusions:
Earlier TTFC is a key indicator of greater nicotine dependence. Knowledge of a smoker's TTFC may allow clinicians to accurately inform smokers of health risks and assign greater resources during cessation attempts. Smokers may be able to use TTFC to self-select cessation aids and accurately assess their unique smoking-related health risks. TTFC may be a better item than cigarettes/day for accurately quantifying dependence and risk in epidemiologic studies.
Pharmacokinetics and Pharmacodynamics of Remimazolam (CNS 7056) after Continuous Infusion in Healthy Male Volunteers
Authors: Schüttler Jürgen , Eisenried Andreas , Lerch Marco , Fechner Jörg , Jeleazcov Christian , Ihmsen Harald 
Published: January 2020

Abstract

Background
Remimazolam (CNS 7056) is a new ultra–short-acting benzodiazepine for intravenous sedation and anesthesia. Its pharmacokinetics and pharmacodynamics have been reported for bolus administration. This study aimed to investigate the pharmacokinetics and pharmacodynamics of remimazolam after continuous infusion.

Methods
Twenty healthy male volunteers (20 to 38 yr, 64 to 99 kg) received remimazolam as continuous intravenous infusion of 5 mg/min for 5 min, 3 mg/min for the next 15 min, and 1 mg/min for further 15 min. Pharmacokinetics of remimazolam and its metabolite were determined from arterial plasma concentrations. Sedation was assessed using the Modified Observer’s Assessment of Alertness and Sedation scale. Pharmacokinetic-pharmacodynamic modeling was performed by population analysis. Hemodynamics and the electrocardiogram were also investigated.

Results
Pharmacokinetics was best described by a three-compartment model for remimazolam and a two-compartment model with transit compartment for the metabolite. Remimazolam showed a high clearance (1.15 ± 0.12 l/min, mean ± SD), a small steady-state volume of distribution (35.4 ± 4.2 l) and a short terminal half-life (70 ± 10 min). The simulated context-sensitive halftime after an infusion of 4 h was 6.8 ± 2.4 min. Loss of consciousness was observed 5 ± 1 min after start, and full alertness was regained 19 ± 7 min after stop of infusion. Pharmacodynamics of Modified Observer’s Assessment of Alertness and Sedation score was best described by a sigmoid probability model with effect site compartment. The half-maximum effect site concentration for a Modified Observer’s Assessment of Alertness and Sedation score less than or equal to 1 was 695 ± 239 ng/ml. The equilibration half-time between central and effect compartment was 2.7 ± 0.6 min. Mean arterial blood pressure decreased by 24 ± 6%, and heart rate increased by 28 ± 15%. Spontaneous breathing was maintained throughout the study. There was no significant prolongation of the QT interval of the electrocardiogram observed.

Conclusions
Remimazolam was characterized by a pharmacokinetic–pharmacodynamic profile with fast onset, fast recovery, and moderate hemodynamic side effects.

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Management of Ankle Arthritis After Severe Ankle Trauma
Authors: Coetzee J. Chris , McGaver Rebecca S. , Seiffert Kayla J. , Giveans M. Russell 
Published: January 2020

Objectives:
There are still unanswered questions about the best treatment options for ankle arthritis after severe lower leg trauma. This study compared results in outcomes and complications for 3 groups.

Design:
Retrospective cohort study case–control study.

Setting:
Single institution.

Patients:
Three groups of 100 surgeries.

Intervention:
Patients underwent an ankle fusion using anterior plate, total ankle arthroplasty (TAA) for primary osteoarthritis (OA), or a TAA for post-traumatic arthritis (PTA).

Main Outcome Measurements:
Veterans Rand 12-Item Health Survey (VR-12), Ankle Osteoarthritis Scale, Visual Analog Pain Scale, and the American Orthopaedic Foot and Ankle Society Hindfoot score were collected preoperatively and at subsequent postoperative appointments. A patient satisfaction survey was also distributed to each patient postoperatively.

Results:
Although all scores improved from preoperative to latest postoperative, the total ankle for PTA consistently resulted in the best postoperative outcomes. Patient satisfaction survey indicated 26% of fusion, 55% of TAA-OA, and 63% of TAA-PTA experienced very good to excellent pain relief. The overall satisfaction was found to be 92.5% for the TAA-PTA patients, 90.5% for the TAA-OA patients, and 84% for the fusion patients. Revision surgeries included 2 from the TAA-OA group, 2 from the TAA-PTA group, and 1 fusion patient.

Conclusions:
Results of comparing 3 groups who underwent an ankle fusion or a TAA demonstrate improvement in pain (P < 0.001), physical quality of life (P < 0.001), and activity (P < 0.001) and resulted in a high level of patient satisfaction.

Level of Evidence:
Level III. See Instructions for Authors for a complete description of levels of evidence.
The Critical Nature of Addressing Burnout Prevention: Results From the Critical Care Societies Collaborative's National Summit and Survey on Prevention and Management of Burnout in the ICU
Authors: Kleinpell Ruth , Moss Marc , Good Vicki S. , Gozal David , Sessler Curtis N. 
Published: January 2020

Objectives:
To summarize the results of expert discussions and recommendations from a National Summit and survey on the promoting wellness and preventing and managing burnout in the ICU.

Data Sources:
Literature review; Critical Care Societies Collaborative (CCSC) Statement on Burnout Syndrome in Critical Care Healthcare Professionals: A Call for Action; CCSC's National Summit on Prevention and Management of Burnout in the ICU; and a descriptive survey on strategies for addressing burnout using Research Electronic Data Capture (REDCap) (project-redcap.org).

Data Synthesis:
Building on the CCSC call for action to address burnout among critical care professionals, the CCSC sponsored the National Summit on Prevention and Management of Burnout in the ICU with 55 invited experts in various fields including psychology, sociology, integrative medicine, psychiatry, suicide prevention, bereavement support, ethics, palliative care, meditation, mindfulness-based stress reduction, among others. Attendees joined breakout groups, to identify factors influencing burnout in ICU professionals and the value of organizational and individual interventions. As a follow-up to the Summit, a descriptive survey assessing strategies for addressing burnout was sent via email or newsletter blast with responses received from 680 CCSC members, including physicians, nurses, pharmacists, therapists, and others.

Conclusions:
The Summit attendees identified the importance of raising awareness among critical care clinicians and key stakeholders, advocating for workplace changes to promote healthy work environments, and promoting research to further explore practical strategies to address, mitigate, and prevent burnout. Critical care clinicians reported that a number of initiatives are being implemented both at their hospitals and at the unit level to build resilience and address burnout prevention. However, other respondents reported that no measures were being used within their organizations, and that colleagues were experiencing burnout. Dissemination and application of resiliency building measures and strategies to address burnout in critical care clinicians are needed.
NRAS Mutation Detected in a Melanoma With Chondroid Stroma: A Case Report With Molecular Evaluation and Literature Review of a Rare Form of Melanoma
Authors: Sweeney Shane P. , Royer Michael C. 
Published: January 2020

Abstract:
Melanoma with cartilaginous differentiation is a rare histologic subtype that has been reported in the literature. It often presents clinically different than conventional melanomas and can be diagnostically challenging. Molecular alterations in previously reported cases have not been published. We present a case of melanoma with chondroid stroma from a 70-year-old man that was found to contain an NRAS mutation (c.182A>G (p.Q61R)) via Illumina TruSight Tumor 15 (TST15) next generation sequencing assay.