bluuebunny/crossref_metadata_2025_split huggingface dataset:
Moderate rate of transmitted resistance mutations to antiretrovirals and genetic diversity in newly HIV-1 patients diagnosed in Benin
Authors: Tchiakpe Edmond , Keke Rene K. , Vidal Nicole , Ahoussinou Clément , Sekpe Olga , Dagba Hermione G. , Gbaguidi Eric , Tonoukouen Conrad , Afangnihoun Aldric , Bachabi Moussa , Gangbo Flore A. , Diop-Ndiaye Halimatou , Toure-Kane Coumba 
Published: July 2020

AbstractObjectiveSeventeen years after the start of the IBAARV (Beninese initiative for access to antiretrovirals), transmitted drug resistance mutations in ARV-naïve patients and HIV-1 genetic diversity were investigated in Benin.ResultsDrug resistance mutations were detected in (27/248; 10.9%) according to the WHO SDRM 2009 list, with a predominance of mutations directed against NNRTIs drugs (24/248; 10%). Phylogenetic and recombination analyses showed a predominance of CRF02_AG strains (165/248; 66.5%) and a high genetic diversity with five other variants and 39 URFs (15.7%) which contained portions of strains that co-circulate in Benin. Eight recent transmission chains revealed active ongoing transmission of HIV-1 strains among ARV-naïve patients. Our study showed a moderate primary drug resistance mutations rate and also provided recent data on the HIV-1 variants that circulate in Benin. Regular monitoring of primary drug resistance is required to adapt HIV-1 treatment strategies and adoption of new WHO recommendations in Benin.
Detecting bracoviral orthologs distribution in five tsetse fly species and the housefly genomes
Authors: Kimenyi Kelvin M. , Abry Muna F. , Okeyo Winnie , Matovu Enock , Masiga Daniel , Kulohoma Benard W. 
Published: July 2020

Abstract
Objective
Mutualism between endogenous viruses and eukaryotes is still poorly understood. Several endogenous double-stranded polydnaviruses, bracoviruses, homologous to those present in parasitic braconid wasp genomes were detected in the tsetse fly (Glossina morsitans morsitans). This is peculiar since tsetse flies do not share a reproductive lifestyle similar to wasps, but deliver fully developed larvae that pupate within minutes of exiting their mothers. The objective of this study is to investigate genomic distribution of bracoviral sequences in five tsetse fly species and the housefly, and examine its value as a potential vector control strategy target point. We use comparative genomics to determine the presence, distribution across Glossina species genomes, and evolutionary relationships of bracoviruses of five tsetse fly species and the housefly.

Results
We report on homologous bracoviruses in multiple Dipteran genomes. Phylogenetic reconstruction using within-species concatenated bracoviral orthologs shows great congruence with previously reconstructed insect species phylogenies. Our findings suggest that bracoviruses present in Diptera originate from a single integration event of the viral genome that occurred in an ancestor insect before the evolutionary radiation of different insect orders.
Functional dissociation of hippocampal subregions corresponding to memory types and stages
Authors: Seok Ji-Woo , Cheong Chaejoon 
Published: July 2020

AbstractBackgroundThe hippocampus reportedly plays a crucial role in memory. However, examining individual human hippocampal-subfield function remains challenging because of their small sizes and convoluted structures. Here, we identified hippocampal subregions involved in memory types (implicit and explicit memory) and stages (encoding and retrieval).MethodsWe modified the serial reaction time task to examine four memory types, i.e. implicit encoding, explicit encoding, implicit retrieval, and explicit retrieval. During this task, 7-T functional magnetic resonance imaging was used to compare brain activity evoked by these memory types.ResultsWe found hippocampal activation according to all memory types and stages and identified that the hippocampus subserves both implicit and explicit memory processing. Moreover, we confirmed that cornu ammonis (CA) regions 1–3 were implicated in both memory encoding and retrieval, whereas the subiculum was implicated only in memory retrieval. We also found that CA 1–3 was activated more for explicit than implicit memory.ConclusionsThese results elucidate human hippocampal-subfield functioning underlying memory and may support future investigations into hippocampal-subfield functioning in health and neurodegenerative disease.
Sow performance in response to natural betaine fed during lactation and post-weaning during summer and non-summer months
Authors: Mendoza S. M. , Boyd R. D. , Remus J. , Wilcock P. , Martinez G. E. , van Heugten E. 
Published: July 2020

AbstractBackgroundTwo studies were conducted to evaluate the effects of dietary natural betaine on sow reproductive performance during summer (Exp. 1) and non-summer months (Exp. 2). Treatments were designed as a 2 × 2 factorial arrangement with factors including dietary betaine (0 or 0.2%) and period of supplementation (lactation or post-weaning until 35 days post-insemination). In Exp. 1, 322 and 327 sows and in Exp. 2, 300 and 327 sows representing young (parity 1 and 2) and mature (parity 3 to 6) sows, respectively, were used.ResultsIn Exp. 1, supplementation of betaine during lactation increased sow body weight losses (− 11.95 vs. −14.63 kg;P = 0.024), reduced feed intake (4.12 vs. 4.28 kg/d;P = 0.052), and tended to reduce percentage of no-value pigs (P = 0.071). Betaine fed post-weaning reduced weaning-to-estrus interval (5.75 vs. 6.68 days;P = 0.054) and farrowing rate (86.74% vs. 91.36%;P = 0.060), regardless of parity group. Post-hoc analysis with sows clustered into 3 parity groups (1, 2 and 3, and 4+) indicated that betaine fed in lactation to parity 4+ sows (P = 0.026) and betaine fed post-weaning to parity 1 sows increased the number of pigs born in the subsequent cycle (P ≤ 0.05). In Exp. 2, betaine fed during lactation tended to reduce the weaning-to-estrus interval (6.64 vs. 7.50 days;P = 0.077) and farrowing rate (88.23% vs. 83.54%;P = 0.089), regardless of parity group. Feeding betaine post-weaning reduced number of pigs born (13.00 vs. 13.64;P = 0.04) and pigs born alive (12.30 vs. 12.82;P = 0.075), regardless of parity group.ConclusionsUsing 0.2% betaine during the non-summer months did not benefit sow performance. During the summer, betaine supplementation in lactation increased subsequent litter size in parity 4+ sows. Betaine fed during the post-weaning period reduced the wean-to-estrus interval and farrowing rate, increased total number of pigs born for parity 1 sows and reduced total number of pigs born to parity 4+ sows. Further research is needed to determine if the detrimental effects on feed intake and farrowing rate may be correlated and depend on dietary betaine level.
Validation of the age-adjusted shock index for pediatric casualties in Iraq and Afghanistan
Authors: Cuenca Camaren M. , Borgman Matthew A. , April Michael D. , Fisher Andrew D. , Schauer Steven G. 
Published: July 2020

AbstractBackgroundPediatric casualties account for a notable proportion of encounters in the deployed setting based on the humanitarian medical care mission. Previously published data demonstrates that an age-adjust shock index may be a useful tool in predicting massive transfusion and death in children. We seek to determine if those previous findings are applicable to the deployed, combat trauma setting.MethodsWe queried the Department of Defense Trauma Registry (DODTR) for all pediatric subjects admitted to US and Coalition fixed-facility hospitals in Iraq and Afghanistan from January 2007 to January 2016. This is a secondary analysis of casualties seeking to validate previously published data using the shock index, pediatric age-adjusted. We then used previously published thresholds to determine patients outcome for validation by age grouping, 1–3 years (1.2), 4–6 years (1.2), 7–12 years (1.0), 13–17 years (0.9).ResultsFrom January 2007 through January 2016 there were 3439 pediatric casualties of which 3145 had a documented heart rate and systolic pressure. Of those 502 (16.0%) underwent massive transfusion and 226 (7.2%) died prior to hospital discharge. Receiver operating characteristic (ROC) thresholds were inconsistent across age groups ranging from 1.0 to 1.9 with generally limited area under the curve (AUC) values for both massive transfusion and death prediction characteristics. Using the previously defined thresholds for validation, we report sensitivity and specificity for the massive transfusion by age-group: 1–3 (0.73, 0.35), 4–6 (0.63, 0.60), 7–12 (0.80, 0.57), 13–17 (0.77, 0.62). For death, 1–3 (0.75, 0.34), 4–6 (0.66–0.59), 7–12 (0.64, 0.52), 13–17 (0.70, 0.57). However, negative predictive values (NPV) were generally high with all greater than 0.87.ConclusionsWithin the combat setting, the age-adjusted pediatric shock index had moderate sensitivity and relatively poor specificity for predicting massive transfusion and death. Better scoring systems are needed to predict resource needs prior to arrival, that perhaps include other physiologic metrics. We were unable to validate the previously published findings within the combat trauma population.
Pseudo-labelling-aided semantic segmentation on sparsely annotated 3D point clouds
Authors: Yao Yasuhiro , Xu Katie , Murasaki Kazuhiko , Ando Shingo , Sagata Atsushi 
Published: July 2020

Abstract
Manually labelling point cloud scenes for use as training data in machine learning applications is a time- and labour-intensive task. In this paper, we aim to reduce the effort associated with learning semantic segmentation tasks by introducing a semi-supervised method that operates on scenes with only a small number of labelled points. For this task, we advocate the use of pseudo-labelling in combination with PointNet, a neural network architecture for point cloud classification and segmentation. We also introduce a method for incorporating information derived from spatial relationships to aid in the pseudo-labelling process. This approach has practical advantages over current methods by working directly on point clouds and not being reliant on predefined features. Moreover, we demonstrate competitive performance on scenes from three publicly available datasets and provide studies on parameter sensitivity.
A randomized trial comparing deep and moderate neuromuscular blockade in patients undergoing ambulatory gynecologic laparoscopy
Authors: Bakhet Wahba Z. 
Published: July 2020

Abstract
Background and aims
Deep neuromuscular blockade (NMB) is known to improve surgical conditions, compared to moderate neuromuscular blockade (NMB), which is expected to improve postoperative quality of recovery (QOR). However, it is unknown whether deep NMB improves postoperative QOR in ambulatory gynecologic laparoscopy. Therefore, we compared the effects of deep and moderate NMB on postoperative QOR in ambulatory gynecologic laparoscopy.

Methodology
We included 80 female in this study. They were randomized into 2 equal groups: deep NMB (dNMB) and moderate NMB (mNMB) at constant pneumoperitoneum pressure of 12 mmHg. The primary outcome was QOR-40 at 24 h, and the secondary outcomes were duration of surgery, surgical rating scale (SRS) score, time to home discharge readiness, pain scores, and tramadol consumption.

Results
The SRS scores were significantly higher in dNMB group, compared with mNMB. Mean (95% CI) SRS scores in deep NMB were 4.55 (4.52-4.58) versus 4.15 (4.11-4.19) in moderate NMB, p = 0.03. However, there was no significant difference between the two groups in the QoR-40 scores, and other secondary outcomes.

Conclusion
We found no difference between deep and moderate NMB on postoperative QOR after ambulatory gynecologic laparoscopy. Therefore, deep NMB during ambulatory gynecologic laparoscopy may be unnecessary, at least in non-obese patients.

Trial registration
This study was registered at www.clinicaltrials.gov (NCT04105764).
Efficacy of nano-formulated certain essential oils on the red flour beetle Tribolium castaneum and confused flour beetle, Tribolium confusum (Coleoptera: Tenebrionidae) under laboratory and storage conditions
Authors: Sabbour Magda Mahmoud Amin 
Published: July 2020

Abtract
Background
The confused flour beetles Tribolium confusum (Jacquelin) and red flour beetle Tribolium castaneum (Herbst) are widespread insects found in the stores and homes that attack many stored foods in several commodities. They are controlled by synthetic chemical insecticides. The natural oils give good results in controlling these pests and decreasing its infestations. The natural essential oils have a low effect on the human, non-target insects, and do not pollute the environments. This research is an alternative approach to naturally control these two stored product pests.

Methods
Nano-formulations of four essential oils, namely, coriander, black seed, caraway oil, and Janesville were tested against T. confusum and T. castaneum in the laboratory and stored conditions. Encapsulation method by the technology of polymerization was followed. The nano-oils were tested at the concentrations of 0.5% against the 3rd larval instars. Cumulative percentages of mortality was determined after 7 days of the first applications. The effect of nano-oils on oviposition was carried out by spraying 2 g of foams of the tested oils and their nano-formulations mixed with 100 g of wheat.

Results
The results obtained showed that the percentage of larval mortality of T. confusum and T. castaneum increased with an increase in the treatment concentrations. Larvae of T. confusum were more susceptible to the treatments than T. castaneum larvae. Nano Janesville oil was more effective than that of coriander or black seed oil. Fecundity and adult emergence percentage are highly suppressed by nano-formulations of coriander, black seed, or Janesville oils under storage conditions, compared to those of the controls or non-nano-oils.
The results suggested that nano Janesville can be used as a valuable tool in pest management programs of T. confusum and T. castaneum

Conclusion
The results of this work indicate that some essential oils might be helpful management of pests in the stores because of their fumigant and odor action. Essential oils of plant origin contain active ingredients that are effective against many insects. Nano-formulations of essential oils, through controlled release, prevent rapid vaporization and degradation and increase the stability of the oils. Treated foam, using coriander, Janesville, caraway, and black seed (habat al Baraka) oils, gave protection against the red flour beetles Tribolium castaneum and confused flour beetle Tribolium confusum.
Retrograde mastoidectomy with canal wall reconstruction with bone graft in acquired cholesteatoma
Authors: Shewel Yasser , Abougabal Ahmed 
Published: July 2020

Abstract
Background
This study aimed to report the surgical outcomes of retrograde mastoidectomy with canal wall reconstruction using a bone graft that secured in place using glass ionomer cement (GIC) in adult patients with acquired cholesteatoma.

Results
This study was conducted on 50 adult patients with acquired cholesteatoma who underwent retrograde mastoidectomy with a reconstruction of canal wall using a bone graft from the mastoid cortex. The preoperative and postoperative audiological evaluation was done; in addition, non-echoplanar (EPI) diffusion-weighted MRI was obtained at least 1 year postoperatively to detect recidivism of cholesteatoma.
Recidivism of cholesteatoma was observed in 4/50 ears (8%) using diffusion-weighted MRI and confirmed during revision mastoidectomy. The audiological assessment showed a marked and statistically significant improvement of preoperative ABG from 30 to 21 dB in addition to the improvement of preoperative AC from 42 to 33 dB. ABG of 20 dB or less was achieved in 50% of the ears. No reaction occurred to GIC in all the ears.

Conclusions
Retrograde mastoidectomy with canal wall reconstruction with bone graft was associated with a low rate of recidivism and significant improvement of the hearing. GIC is safe and effective in stabilization of bone graft in canal wall reconstruction.
Analysis of GABRB3 gene mRNA expression and motor coordination after administration of valerian extracts (Valeriana officinalis) in BALB/c mice
Authors: Mulyawan Erwin , Ahmad Muhammad Ramli , Islam Andi Asadul , Massi Muh. Nasrum , Hatta Mochammad , Arif Syafri Kamsul 
Published: July 2020

Background: Valeriana officinalis has often been used to treat sleep disorders as a traditional medicine for 2000 years. The sedative effect of valerian extract is facilitated by the GABAA receptor β3 subunit. The aim of this study is to determine the effect of valerian extract on GABRB3 gene mRNA expression and sedative effect in BALB/c mice. Methods: This is an experimental preclinical study using a posttest-only control group design. A total of 20 BALB/c mice were randomly allocated into four groups consisting of five mice each. Group I was given 5 ml of Aqua Dest (distilled water), group II was given 0.025 mg/10 g of diazepam, group III was given 2.5 mg/10 g of valerian extract, and group IV was given 5 mg/10 g of valerian extract. The drugs were administrated for seven days through a gastric gavage. The rotarod test was performed on the seventh day. A blood sample was taken on the first day before drug administration and after the rotarod test on the seventh day to be analyzed using quantitative real-time PCR. Results: GABRB3 gene mRNA expression showed a significant increase in groups II, III, and IV (p <0.0001). There was significant difference between group III and IV. The examination of motor coordination (rotarod test) showed a significant difference (p <0.05) between group I and group II, between group I and group III, and between group I and group IV. There was no significant difference between group II and both groups III and IV. Conclusions: GABRB3 gene mRNA expression was significantly increased after the administration of valerian extract. Based on the rotarod test, valerian extract and diazepam had a clinically similar sedation effect. A higher dose of valerian extract does not yield a higher level of GABRB3 gene mRNA expression nor sedative effects.
Case Report: Cutaneous chilblains-like lesions (CCLL) versus COVID-19 toes during the pandemic. Confocal findings and proposal for new acronym, CCLL – is there a diagnostic window?
Authors: Ludzik Joanna , Witkowski Alexander , Hansel Donna E. , Raess Philipp W. , White Kevin , Leachman Sancy 
Published: July 2020

The COVID-19 outbreak caused by the novel coronavirus, SARS-CoV-2, typically presents with symptoms including fever, cough, headache, myalgia, asthenia, anosmia, diarrhea, and sometimes pneumonia, which can be fatal. Recently, new dermatologic findings have been described in association with the disease that can potentially be a distinguishing feature of infection. One such feature resembles chilblains and this case report represents a unique presentation of this feature.
What Will Help the Teacher to Prepare Students in Successfully Solving PISA Assignments
Authors: Usova S. 
Published: July 2020

Modern life sets its priorities: not a simple knowledge of facts, not skills as such, but the ability to use what is acquired; not the amount of information, but the ability to get it and model; not consumerism, but creation and cooperation. Possession of these competencies by fifteen-year-old students is assessed in the international PISA study.
To solve one of the key tasks of the national project "Education" - by 2024 to enter the TOP-10 countries in the quality of general education, it is important to answer the question: What should a teacher know and be able to prepare students for successful solution of PISA tasks?
The article discusses the main approaches to assessing the functional literacy of students in the framework of the international PISA study, reveals the features and key characteristics of the competencies of each of the main areas of the PISA study. Methodological approaches to prepare students for solving PISA tasks in the classroom and extracurricular activities are given.
The author focuses teachers on the necessary to master of certain professional competencies.
Spanning Tests for Assets with Option-Like Payoffs: The Case of Hedge Funds
Authors: Karehnke Paul , de Roon Frans 
Published: July 2020

We draw on the skewness literature to propose regression-based performance evaluation tests designed for investments with option-like returns. These tests deliver conclusions valid for all risk-averse mean-variance-skewness investors and can better account for nonlinearities in returns than option-based factor models. Applied to mutual and hedge funds, our tests usually suggest selecting different funds than standard tests and find that a significant fraction (11%) of hedge funds adds value to investors, whereas this is an insignificant 4% for mutual funds. We also analyze the economic significance of these option-like returns and their out-of-sample persistence. This paper was accepted by Tyler Shumway, finance.
Vitamin D₃ levels in the maternal serum, cord blood, and placenta of preeclamptic pregnant women
Authors: Wibowo Noroyono , Irwinda Rima , Handoko Yohanes 
Published: July 2020

BACKGROUND Preeclampsia is affected by oxidative stress, a free-radical produced as a by-product of endothelial damage, and antioxidant imbalance, such as vitamin D₃. This study was aimed to compare the vitamin D₃ levels in the placenta, cord blood, and maternal serum between patients with and without preeclampsia.
METHODS This cross-sectional study included 86 patients from Cipto Mangunkusumo Hospital and Tangerang District Hospital, in which 47 had preeclampsia (13 early-onset and 16 late-onset preeclampsia cases) and 39 had no preeclampsia. The placenta, cord blood, and maternal serum were taken after labor, then were analyzed according to preeclampsia and non-preeclampsia; furthermore, the preeclampsia group was analyzed in a subgroup of early- and late-onset preeclampsia. This is analyzed with either unpaired t-test, Mann–Whitney U test, or Kruskal–Wallis test.
RESULTS The maternal serum, cord blood, and placental tissue vitamin D₃ levels (16.30 [6.20–49.00], 11.80 [3.50–38.60], and 49.00 [22.00–411.00] ng/ml, respectively) of the preeclampsia group were similar to those of the non-preeclampsia group (13.50 [4.80– 29.20], 11.70 [1.00–28.80], and 43.40 [11.80–153.00] ng/ml, respectively) (p = 0.459, 0.964, and 0.354, respectively). However, the placental tissue vitamin D₃ levels in early-onset preeclampsia (79.00 [36.00–411.00] ng/ml) were higher than those in late-onset preeclampsia (40.00 [22.00–171.00] ng/ml) (p = 0.006).
CONCLUSIONS The vitamin D₃ levels between patients with and without preeclampsia were similar. However, the placental tissue vitamin D₃ levels in early-onset preeclampsia were higher than those in late-onset preeclampsia, possibly because of the different pathophysiology between early- and late-onset preeclampsia.