bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is the Glenoid Vault Outer Cortex&nbsp;a more Accurate Reference Plane than Conventional Methods in Shoulder Arthroplasty?
Authors: Gregory Thomas , Hurst Simon , Merlini Lorenzo , Hansen Ulrich , Gregory Jules , Emery Roger 
Published: October 2020

Abstract
Purpose Glenoid component positioning is an important determinant of outcome in anatomic shoulder arthroplasty. This is dependent on the accurate preparation of bony surfaces. We describe and assess a novel plane for improving the accuracy of bony preparation - the Glenoid Vault Outer Cortex plane (GvOC). Research question Does the GvOC plane provide a more accurate representation of glenoid version and inclination than the standard scapular border (SB) method ? Methods 105 CT scans of normal scapulae were obtained. 46 females and 59 males, aged between 22 to 30 years. Accuracy of the GvOC was compared against the current ‘gold standard’ – the SB method. Measurements of glenoid inclination, version, rotation, and offset were made using both GvOC and SB planes. These were compared to 'actual values' obtained using an alternative method. Results The mean difference between estimates of version based on the GvOC plane and the reference value were 1.8° (-2 to 5, SD 1.6) as compared to 6.7° (-2 to 17, SD 4.3) when the SB plane was used, (p<0.001). The mean difference between estimates of inclination based on the GvOC plane and the reference value were 1.9° (-4 to 6, SD 1.6) as compared to 11.2° (-4 to 25, SD 6.1) when the SB plane was used, (p<0.001). Conclusions The GvOC plane produced estimates of genoid version and inclination closer to the actual with a lower variance than using the standard SB plane. This may provide a more accurate and reproducible method for surgeons when defining native glenoid anatomy.
Hip Geometry in Hip Fracture Patients in Greenland
Authors: Fleischer Inuuteq , Laursen Mogens , Andersen Stig 
Published: October 2020

Abstract
Background: Hip geometry influences hip fracture risk. Hip fractures are common and they are associated with pain, disability, premature death and marked costs on society. Osteoporotic fractures are frequent in Arctic populations and increase with advancing age in this society that has a steep rise in life expectancy. Greenland Inuit is a distinct ethnic group and data on hip geometry is missing. This let us to describe hip geometry in populations with hip fracture in Greenland.Methods: We evaluated collodiaphysial angle, femoral neck length, the outer and inner diameter of the femur at 2 and 5 centimetres below the centre of the lesser trochanter and the cortical thickness from pelvic and hip radiographs in all patients operated in Greenland over a 7.7-year period. We included all 84 patients with one non-fractured hip visible for geometric analysis. Analyses were conducted in duplicate.Results: We found collodiaphysial angle of 134.8/132.6o in men/women (p=0.06) and femoral neck length of 38.0/33.9mm in men/women (p=0.001). Cortical thickness was influenced by sex in the adjusted analysis (p<0.001). Cortical thickness index at 5cm below the centre of the lesser trochanter decreased with age (p=0.026) and may be influenced by height (2cm below the centre of the lesser trochanter, p=0.053). Conclusion: Our findings differed from European reference data and suggest a delicate balance in hip geometry that may change with lifestyle transition in Arctic populations. Ethnic peculiarities influence the hip structure and may have an impact on fracture risk. A focus on hip geometry and risk factors for osteoporotic fractures in Arctic populations is warranted.
Characterizing the Permeability and Methane Release with the Treatment of Pressure-relief Mining: Intensive Field Measurements
Authors: Zhang Cun , Bai Qingsheng , Song Ziyu , Luo Yang , Chen Jianhang 
Published: October 2020

Abstract
Characterizing the permeability evolution and methane release is of great significance for the safe mining of the high gas outburst seams, as well as coal and gas simultaneous extraction. Theoretical analysis, laboratory testing, and numerical simulation are widely used methods to characterize the permeability and methane release with the treatment of pressure-relief mining. However, these methods cannot fully reflect the complexity of filed practice. In this study, we report the effectiveness of protective coal seam (PCS) mining and the pressure-relief area in the protected coal seam (PDCS) based on detailed and integrated field measurements in a Chinese coal mine. To the best of our knowledge, it is the first time to measure the permeability coefficient and gas pressure evolution in the PDCS during the PCS longwall mining. The evolution of the permeability coefficient in the pressure-relief area during PCS mining can be divided into four stages: slowly decreasing, sharply increasing, gradually decreasing, and basically stable. The maximum permeability coefficient is 322 times of the initial value and finally stabilized at 100 times after the goaf compacted. The gas pressure evolution in the PDCS indicates that the strike pressure relief angle is 52.2° at the active longwall face zone, and 59.3° at the installation roadway side. The tendency pressure relief angles at the lower and upper sides of the longwall panel are 75° and 78.9°, respectively. The residual gas content and gas pressure of the PDCS in the pressure-relief area are reduced to less than 6 m3/t and within 0.4 MPa, respectively. The field measurements show that pressure-relief mining can prevent coal and gas outbursts in PDCSs. The field observations in this paper can serve as benchmark evidence for theoretical analysis and numerical simulations, and also provide insights into realizing safety mining in similar conditions.
Genetic Map of Relapsed and Refractory Diffuse Large B-cell Lymphoma: A Systemic Review and Association Analysis
Authors: Gao Fan , Tian Lei , Wang Jing , Dong Fei , Hu Kai , Ke Xiaoyan 
Published: October 2020

Abstract
Diffuse large B-cell lymphoma (DLBCL) is the most common histological subtype of non-Hodgkin lymphoma (NHL). In recent years, a deeper understanding of the genetic subtypes of diffuse large B lymphoma has been reached, and these advances have also been applied to research on relapsed and refractory diffuse large B-cell lymphoma (RRDLBCL). We screened 1495 documents, compiled the whole-exome sequencing data of several studies, formed a data set including 92 observations, and performed association analysis on the high-frequency mutations among them. The most common mutations in the data set include TTN (34/92, 37.0%), KMT2D (29/92, 31.5%), TP53 (25/92, 27.2%), IGLL5 (25/92, 27.2%), CREBBP (21 /92, 22.8%), BCL2 (21/92, 22.8%), MYD88 (20/92, 21.7%), and SOCS1 (19/92, 20.7%). Among these, CREBBP, KMT2D, and BCL2 have a strong association with each other, and SOCS1 has a strong association with genes such as ACTB, CIITA, and GNA13. There is also a strong association between SOCS1 and STAT6. Though TP53 and MYD88 lack significant associations with most genes, the association between MYD88 and PIM1 is significant. Through SOM clustering and expression-level analysis of common gene mutations, we believe that RRDLBCL can be divided into four main types: (1) JAK-STAT-related type, including STAT6, SOCS1, ITPKB, CIITA, and B2M. The expression lineage is similar to PMBL and cHL. (2) EZB type: BCL2 and EZHZ are the main types of mutations. Epigenetic mutations such as KMT2D and CREBBP are more common in this type, and are often accompanied by BCL2 mutations. (3) MCD type, including MYD88, CD79B and PIM1. These genes are involved in the BCR signaling pathway and related pathways, and are connected by the common NF-κB pathway. (4) Undefined type (Sparse Mutation type). These patients are mainly individuals with sparse mutations, including some patients with TP53 mutations (30.3%, 10/33), but who generally lack characteristic mutations. Among the common gene mutations, the expression changes in BCL2, PIM1, STAT6, ITPKB, and GNA13 have more significant prognostic significance. We also reviewed the literature from recent years concerning the previously mentioned common gene mutations.
Potential of Three-step Pretargeting Radioimmunotherapy Using Biotinylated Bevacizumab and Succinylated Streptavidin in Triple-negative Breast Cancer Xenograft&nbsp;
Authors: Gu Wenchao , Yudistiro Ryan , Hanaoka Hirofumi , Katsumata Natsumi , Tsushima Yoshito 
Published: October 2020

Abstract
Background Pretargeting radioimmunotherapy (PRIT) is a promising approach that can reduce long-time retention of blood radioactivity and consequently reduce hematotoxicity. Among the PRIT strategies, the combination of biotin-conjugated mAb and radiolabeled streptavidin (StAv) is a simple and convenient method because of ease of preparation. This study performed three-step (3-step) PRIT using the sequential injection of (1) biotinylated bevacizumab (Bt-BV), (2) avidin, and (3) radiolabeled StAv for the treatment of triple-negative breast cancer (TNBC). Methods Four biodistribution studies were performed using 111In in tumor-bearing mice to optimize each step of our PRIT methods. Further, a therapeutic study was performed with optimized 3-step PRIT using 90Y-labeled StAv. Results Based on the biodistribution studies, the protein dose of Bt-BV and avidin was optimized to 100 μg and 10 molar equivalent of BV, respectively. Succinylation of StAv significantly decreased the kidney accumulation level (with succinylation (6.96 ± 0.91) vs without succinylation (20.60 ± 1.47) at 1 h after injection, p < 0.0001) with little effect on tumor accumulation level. In the therapeutic study, tumor growth was significantly suppressed in treatment groups with optimized 3-step PRIT using 90Y-labeled succinylated StAv compared to that of the no-treatment group (p < 0.05). Conclusion The 3-step PRIT strategy of this study achieved fast blood clearance and low kidney uptake with little effect on tumor accumulation level, and a certain level of therapeutic effect was consequently observed. These results indicated that the pretargeting treatment of the current study may be effective for human TNBC treatment.
Prognostic Stratification of Baseline Total Metabolic Tumor Volume on PET/CT Combined with MYC/Bcl-2 Dual Expression in Patients with Primary Gastrointestinal Diffuse Large B-Cell Lymphoma
Authors: Jiang Chong , Teng Yue , Chen Jieyu , Zheng Zhong , Wang Zhen , Zhou Zhengyang , Xu Jingyan , Ding Chongyang 
Published: October 2020

Abstract
Purpose: The aim of the present study was to explore whether pretreatment total metabolic tumor volume (TMTV) combined with MYC/BCL-2 dual expression (DE) would improve the prognostic stratification of patients who were newly diagnosed with primary gastrointestinal diffuse large B-cell lymphoma (PGI-DLBCL).Materials and methods: Eighty-three patients between March 2011 and November 2019, diagnosed with PGI-DLBCL prior to treatment, were included in this retrospective study. Baseline TMTV on PET/CT scans were calculated automatically using the boundaries of voxels presenting a SUVmax≥2.5. Expression of MYC/BCL-2 were detected at protein levels via immunohistochemistry（IHC）. The distributions of Progression-free survival (PFS) and overall survival (OS) rates were estimated using the Kaplan-Meier method and differences were compared using a log-rank test followed by multivariate analysis using the Cox proportional hazards model.Results: TMTV and DE were significantly associated with a worse PFS and OS. Multivariate analysis revealed that TMTV (HR=6.090, P<0.001) and DE (HR=2.761, P=0.021) were each independent predictors of PFS, whereas TMTV (HR=9.512, P<0.001) was the only independent predictor of OS. A monogram comprised of TMTV and DE expression identified four groups with very different outcomes: (PFS:χ2=32.178, P<0.001; OS:χ2=23.091, P<0.001): low-risk group (low TMTV and non-DE, 46 patients); low-intermediate risk group (low TMTV and DE, 16 the patients); high-intermediate risk group (high TMTV and non-DE, 12 patients); and high-risk group (high TMTV and DE patients, 9 patients). Conclusions: TMTV and DE independently predicted survival outcomes in PGI-DLBCL patients. Furthermore, our findings suggest that combination of TMTV and DE may further improve the ability of clinicians to stratify patients in terms of differential prognoses.
Photo-excited Toluidine Blue Disaggregates the Repeat Tau and Modulates End-binding Protein EB1, Cytoskeletal Structure in Neuronal Cells
Authors: Dubey Tushar , Gorantla Nalini Vijay , Chinnathambi Subashchandrabose 
Published: October 2020

Abstract
BackgroundAlzheimer’s disease is a progressive neurological disorder characterized by the intracellular accumulation of Tau protein aggregates. Inhibition of protein aggregation by photo-excited dyes is emerging as a novel strategy for the treatment of certain diseases. Toluidine Blue is a basic phenothiazine dye having the potency of photo-excitation by irradiation with a red light at 630±20 nm. In the present work, we studied the effect of Toluidine Blue and photo-excited Toluidine Blue on the aggregation of repeat Tau using in-vitro assays. ResultsResults showed that Toluidine Blue efficiently inhibited the formation of higher-order aggregates which was evidenced by Thioflavin S fluorescence assay, SDS-PAGE and electron microscopy. Moreover, the photo-excited Toluidine Blue led to disaggregation of the mature repeat Tau fibrils, which were irradiated in the dark chamber customized in our lab. Further, studies on the effect of Toluidine blue on cell viability in Neuro2a cells using MTT assay showed that Toluidine Blue was not toxic to neuronal cells at lower concentrations but at high concentrations (> 5 µM) both Toluidine Blue and photo-excited Toluidine Blue induced significant toxicity. Immunofluorescence studies on the cytoskeleton of Neuro2a cells show that Toluidine Blue and photo-excited Toluidine Blue treatment at a non-toxic concentration of 0.5 µM stimulated the formation of actin-rich lamellipodia and filopodia structures. Tubulin networks were also differentially modulated after the treatment of Toluidine Blue and photo-excited Toluidine Blue. End-binding protein 1 (EB1) levels were observed to increase after Toluidine Blue and photo-excited Toluidine Blue treatment indicating the accelerated microtubule polymerization. The overall study suggested that Toluidine Blue inhibited the aggregation of soluble Tau and photo-excited Toluidine Blue disaggregated the pre-formed Tau filaments. ConclusionsIn our study, TB and PE-TB were observed to be potent against Tau aggregation. We observed a distinctive modulation of actin, tubulin networks and EB1 levels after TB and PE-TB treatment, which clearly suggested that TB and PE-TB have potency against the cytoskeleton deformities. Thus, from the present work, we are stating that TB could be a potent molecule against Tauopathy.
Efficacy of Dietary Interventions on Weight Loss and Biochemical Parameters Among Saudi Females with Obesity and Overweight: An Interventional Non-Randomized Control Trail.
Authors: Aldisi Dara , Afsar Tayyaba , Al-farraj Amani Abdullah , Alothman Alaa Fahad , Al-Mokyel Shajan Hassan , AlQahtani Shahad Abdullah , Bakhsh Mariam yousef , Alenazy Nawarah Ali , Razak Suhail , Elshafie Mona Mohamed , Almajwal Ali 
Published: October 2020

Abstract
Background: Numerous dietary approaches have been proposed to treat obesity. We investigated the efficacy of the Ideal Protein Weight Loss Method (IPWLM) diet and the balanced diet (BD) on anthropometric, body composition, and biochemical parameters in obese/overweight subjects. Methods: 60 overweight/obese females from Riyadh, Saudi Arabia were divided into two groups: Group IPWLM (n=29) followed the IPWLM diet, while the BD group (n=31) received a balanced diet for 6 weeks. Result: Significant reduction in anthropometric measurements and body composition were observed in BD and IPWLM groups. In both BD and IPWLM groups, all weight, % body fat (BF), % muscle mass, hip and waist circumferences, and body mass index (BMI) had a significant reduction (p, for all trends <0.05). BD group subjects had a significant reduction in triglycerides (TG) and cholesterol (p <0.05), while no changes in HDL and glucose (p, for all trends >0.05). IPWLM group showed no significant effects on blood glucose and lipid profile (LDL, HDL, and cholesterol (p >0.05). Conclusion: Although the IPWLM diet did not improve biochemical parameters, it was significantly associated with improvements in anthropometrics, %BF, and muscle mass preservation. On the other hand, BD was more favorable in anthropometrics, body composition as well as biochemical parameters.
Chinese Women’s Attitudes Towards Postpartum Interventions to Prevent Type 2 Diabetes After Gestational Diabetes: A Semi-Structured Qualitative Study
Authors: Shang Jie , Henry Amanda , Zhang Puhong , Chen Huan , Thompson Kelly , Wang Xiaodong , Liu Na , Zhang Jiani , Liu Yan , Pan Xiongfei , Yang Xue , Jin Jianbo , Hirst Jane Elizabeth 
Published: October 2020

Abstract
Background: Gestational diabetes (GDM) is a global problem affecting millions of pregnant women, including in mainland China. These women are at high risk of Type II diabetes (T2DM). Cost-effective and clinically effective interventions are needed. We aimed to explore Chinese women’s perspectives, concerns and motivations towards participation in early postpartum interventions and/or research to prevent the development of T2DM after a GDM-affected pregnancy. Methods: We conducted a qualitative study in two hospitals in Chengdu, Southwest China. Face-to-face semi-structured interviews were conducted with 20 women with recent experience of GDM: 16 postpartum women and 4 pregnant women. Women were asked about their attitudes towards postpartum screening for type 2 diabetes, lifestyle interventions, mHealth delivered interventions and pharmacologic interventions (specifically metformin). An inductive approach to analysis was used. Interviews were recorded, transcribed, and coded using NVivo 12 Pro. Results: Most women held positive attitudes towards participating in T2DM screening, and were willing to participate in postpartum interventions to prevent T2DM through lifestyle change or mHealth interventions. Women were less likely to agree to pharmacological intervention, unless they had family members with diabetes or needed medication themselves during pregnancy. We identified seven domains influencing women’s attitudes towards future interventions: (1) experiences with the health system during pregnancy; (2) living in an enabling environment; (3) the experience of T2DM in family members; (4) knowledge of diabetes and perception of risk; (5) concerns about personal and baby health; (6) feelings and emotions, and (7) lifestyle constraints. Those with more severe GDM, an enabling environment and health knowledge, and with experience of T2DM in family members expressed more favourable views of postpartum interventions and research participation to prevent T2DM after GDM. Those who perceived themselves as having mild GDM and those with time/lifestyle constraints were less likely to participate.Conclusions: Women with experiences of GDM in Chengdu are generally willing to participate in early postpartum interventions and/or research to reduce their risk of T2DM, with a preference for non-drug, mHealth based interventions, integrating lifestyle change strategies, blood glucose monitoring, postpartum recovery and mental health.
Successful Surgical Strategy for Graft-esophageal Fistula After Total Arch Replacement : A Case Report.
Authors: Munakata Hiroshi , Yasumori Ken , Shimabukuro Noriko , Yamazato Takahiro , Abe Noriyuki , Nakamura Tetsu 
Published: October 2020

Abstract
BackgroundGraft infection is one of the catastrophic complications in the aortic surgical area. Case presentationA 62-year-old man was diagnosed graft-esophageal fistula after total arch replacement. We have experienced aggressive debridement, that includes the infected previous graft and unhealthy esophagus under the same operative field, and staged esophageal reconstruction was performed 4 months later. ConclusionsWe believe that this successful surgical strategy, which involves aggressive debridement using re-median incision plus a left posterolateral thoracotomy under the same operative field, is useful and safe and achieved a favorable result.
Co-existence of Cohen Syndrome and Pendred Syndrome? Diagnostic Challenges Associated With Presence of Multiple Genomic Variants in the Newborn: A Case Report
Authors: Li Guoxu , Mu Yu , Sun Chao , Zheng Jie , Xu Xiaowei , Yan Xiaodan , Zhang Fang , Shu Jianbo , Liu Yang 
Published: October 2020

Abstract
BackgroundCohen syndrome is a multisystem autosomal recessive hereditary disease, which is caused by variants of the VPS13B gene. The clinical manifestations include characteristic facial features, microcephaly, trunk obesity and mental retardation. The SLC26A4 gene encodes an anion transporter called Pendrin, the variants of SLC26A4 lead to deafness, Pendred syndrome and enlarged vestibular aqueduct. We presence a case of multiple genetic homozygous variants of SLC26A4 and VPS13B.Case presentationIn this study, we described a case of a 65-day-old female infant presenting with hearing loss and poor feeding. The deafness gene screening before admission showed that the patient carried homozygous c.919-2A>G variation of SLC26A4 gene. The symptoms of the patient did not improve significantly after a period of targeted treatment, then the possibility of genetic diseases was considered after consultation with the different departments. Whole exome sequencing was performed, and homozygous splicing variants in intron 38 (c.6940+1G>T) of VPS13B and that in intron 7 (c.919-2A>G) of SLC26A4 were identified. Later, the parent was proved as the carriers of VPS13B and SLC26A4 heterozygous variations by Sanger sequencing.ConclusionsPresence of multiple genetic homozygous variants of SLC26A4 and VPS13B, it is difficult to make an early diagnosis due to early atypical symptoms. Final diagnosis depends on whole exome sequencing which plays an important role in early diagnosis of intractable neonatal cases.
SeqEnhDL: sequence-based classification of cell type-specific enhancers using deep learning models
Authors: Wang Yupeng , Jaime-Lara Rosario , Roy Abhrarup , Sun Ying , Liu Xinyue , Joseph Paule 
Published: October 2020

Abstract
ObjectiveComputational identification of cell type-specific regulatory elements on a genome-wide scale is very challenging.ResultsWe propose SeqEnhDL, a deep learning framework for classifying cell type-specific enhancers based on sequence features. DNA sequences of “strong enhancer” chromatin states in nine cell types from the ENCODE project were retrieved to build and test enhancer classifiers. For any DNA sequence, sequential k-mer (k=5, 7, 9 and 11) fold changes relative to randomly selected non-coding sequences were used as features for deep learning models. Three deep learning models were implemented, including multi-layer perceptron (MLP), Convolutional Neural Network (CNN) and Recurrent Neural Network (RNN). All models in SeqEnhDL outperform state-of-the-art enhancer classifiers including gkm-SVM and DanQ, with regard to distinguishing cell type-specific enhancers from randomly selected non-coding sequences. Moreover, SeqEnhDL is able to directly discriminate enhancers from different cell types, which has not been achieved by other enhancer classifiers. Our analysis suggests that both enhancers and their tissue-specificity can be accurately identified according to their sequence features. SeqEnhDL is publicly available at https://github.com/wyp1125/SeqEnhDL.