bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pituitary Macroadenoma Presenting With Monocular Temporal Hemianopia
Authors: Gupta Ankur , Deshmukh Mira , Palexas George 
Published: November 2020

Abstract:
A 35-year-old primigravida woman presented to the eye emergency department with reduced visual acuity in the right eye. Humphrey visual field testing showed a monocular right eye temporal hemianopia before delivery. An MRI after delivery revealed a largely symmetrical pituitary macroadenoma with chiasmal compression. This is a rare presentation of a pituitary macroadenoma especially when the tumor is largely symmetrical.
Horizontal Gaze Palsy, Scoliosis, and Split Pons Sign in a 6-Year-Old Girl
Authors: Singanamalla Bhanudeep , Chaurasia Shweta , Jain Chirag , Bhatia Vikas , Sharma Neeraj , Madaan Priyanka , Gopinathan Nirmal Raj , Saini Lokesh 
Published: November 2020

Abstract:
A 6-year-old girl presented with complaints of absent horizontal eye movements since birth. There was also associated progressive scoliosis for past 1 year. Neuroimaging revealed split pons sign, butterfly-shaped medulla, and prominent inferior olivary nuclei. The presence of congenital horizontal gaze palsy, childhood onset progressive scoliosis, and abnormal neuroimaging findings confirmed the diagnosis of horizontal gaze palsy with progressive scoliosis. This case highlights the importance of neuroimaging in a child presenting with horizontal gaze palsy and scoliosis that helped for starting early rehabilitation of the child, prevention of permanent vision loss, and parental counseling for future pregnancies.
Facial Nerve Involvement Accompanying Optic Neuritis Secondary to Myelin Oligodendrocyte Glycoprotein Antibody–Associated Disorder
Authors: Chen Zhiyong , Saini Monica , Loo Jing Liang 
Published: November 2020

Abstract:
We present the first reported case of facial nerve involvement accompanying an optic neuritis in myelin oligodendrocyte glycoprotein antibody–associated disorder.
Temperature variability alters the stability and thresholds for collapse of interacting species
Authors: Dee Laura E. , Okamtoto Daniel , Gårdmark Anna , Montoya Jose M. , Miller Steve J. 
Published: November 2020

Temperature variability and extremes can have profound impacts on populations and ecological communities. Predicting impacts of thermal variability poses a challenge, because it has both direct physiological effects and indirect effects through species interactions. In addition, differences in thermal performance between predators and prey and nonlinear averaging of temperature-dependent performance can result in complex and counterintuitive population dynamics in response to climate change. Yet the combined consequences of these effects remain underexplored. Here, modelling temperature-dependent predator–prey dynamics, we study how changes in temperature variability affect population size, collapse and stable coexistence of both predator and prey, relative to under constant environments or warming alone. We find that the effects of temperature variation on interacting species can lead to a diversity of outcomes, from predator collapse to stable coexistence, depending on interaction strengths and differences in species' thermal performance. Temperature variability also alters predictions about population collapse—in some cases allowing predators to persist for longer than predicted when considering warming alone, and in others accelerating collapse. To inform management responses that are robust to future climates with increasing temperature variability and extremes, we need to incorporate the consequences of temperature variation in complex ecosystems.This article is part of the theme issue ‘Integrative research perspectives on marine conservation’.
Citizen science and marine conservation: a global review
Authors: Kelly Rachel , Fleming Aysha , Pecl Gretta T. , von Gönner Julia , Bonn Aletta 
Published: November 2020

Climate change, overfishing, marine pollution and other anthropogenic drivers threaten our global oceans. More effective efforts are urgently required to improve the capacity of marine conservation action worldwide, as highlighted by the United Nations Decade of Ocean Science for Sustainable Development 2021–2030. Marine citizen science presents a promising avenue to enhance engagement in marine conservation around the globe. Building on an expanding field of citizen science research and practice, we present a global overview of the current extent and potential of marine citizen science and its contribution to marine conservation. Employing an online global survey, we explore the geographical distribution, type and format of 74 marine citizen science projects. By assessing how the projects adhere to the Ten Principles of Citizen Science (as defined by the European Citizen Science Association), we investigate project development, identify challenges and outline future opportunities to contribute to marine science and conservation. Synthesizing the survey results and drawing on evidence from case studies of diverse projects, we assess whether and how citizen science can lead to new scientific knowledge and enhanced environmental stewardship. Overall, we explore how marine citizen science can inform current understanding of marine biodiversity and support the development and implementation of marine conservation initiatives worldwide.This article is part of the theme issue ‘Integrative research perspectives on marine conservation’.
Mechanism of ligand recognition by human ACE2 receptor
Authors: Bhattarai Apurba , Pawnikar Shristi , Miao Yinglong 
Published: November 2020

AbstractAngiotensin converting enzyme 2 (ACE2) plays a key role in renin-angiotensin system regulation and amino acid homeostasis. Human ACE2 acts as the receptor for severe acute respiratory syndrome coronaviruses SARS-CoV and SARS-CoV-2. ACE2 is also widely expressed in epithelial cells of lungs, heart, kidney and pancreas. It is considered an important drug target for treating SARS-CoV-2, as well as pulmonary diseases, heart failure, hypertension, renal diseases and diabetes. Despite the critical importance, the mechanism of ligand binding to the human ACE2 receptor remains unknown. Here, we address this challenge through all-atom simulations using a novel ligand Gaussian accelerated molecular dynamics (LiGaMD) method. Microsecond LiGaMD simulations have successfully captured both binding and unbinding of the MLN-4760 inhibitor in the ACE2 receptor. In the ligand unbound state, the ACE2 receptor samples distinct Open, Partially Open and Closed conformations. Ligand binding biases the receptor conformational ensemble towards the Closed state. The LiGaMD simulations thus suggest a conformational selection mechanism for ligand recognition by the ACE2 receptor. Our simulation findings are expected to facilitate rational drug design of ACE2 against coronaviruses and other related human diseases.
Understanding The Role of Magnetic and Magneto-Quasistatic Fields in Human Body Communication
Authors: Nath Mayukh , Ulvog Alfred Krister , Weigand Scott , Sen Shreyas 
Published: November 2020

AbstractWith the advent of wearable technologies, Human Body Communication (HBC) has emerged as a physically secure and power-efficient alternative to the otherwise ubiquitous Wireless Body Area Network (WBAN). Whereas the most investigated nodes of HBC have been Electric and Electro-quasistatic (EQS) Capacitive and Galvanic, recently Magnetic HBC (M-HBC) has been proposed as a viable alternative. Previous works have investigated M-HBC through an application point of view, without developing a fundamental working principle for the same. In this paper, for the first time, a ground up analysis has been performed to study the possible effects and contributions of the human body channel in M-HBC over a broad frequency range (1kHz to 10 GHz), by detailed electromagnetic simulations and supporting experiments. The results show that while M-HBC can be successfully operated as a body area network, the human body itself plays a minimal or negligible role in it’s functionality. For frequencies less than∼30 Hz, in the domain of operation of Magneto-quasistatic (MQS) HBC, the human body is transparent to the quasistatic magnetic field. Conversely for higher frequencies, the conductive nature of human tissues end up attenuating Magnetic HBC fields due to Eddy currents induced in body tissues, eliminating the possibility of the body to support efficient waveguide modes. With this better understanding at hand, different modes of operations of MQS HBC have been outlined for both high impedance capacitive and 50Ω termination cases, and their performances have been compared with EQS HBC for similar sized devices, over varying distance between TX and RX. The resulting report presents the first fundamental understanding towards M-HBC operation and its contrast with EQS HBC, aiding HBC device designers to make educated design decisions, depending on mode of applications.
Viral genome sequencing places White House COVID-19 outbreak into phylogenetic context
Authors: Bedford Trevor , Logue Jennifer K. , Han Peter D. , Wolf Caitlin R. , Frazar Chris D. , Pelle Benjamin , Ryke Erica , Hadfield James , Lee Jover , Rieder Mark J. , Nickerson Deborah A. , Lockwood Christina M. , Starita Lea M. , Chu Helen Y. , Shendure Jay 
Published: November 2020

AbstractIn October 2020, an outbreak of at least 50 COVID-19 cases was reported surrounding individuals employed at or visiting the White House. Here, we applied genomic epidemiology to investigate the origins of this outbreak. We enrolled two individuals with exposures linked to the White House COVID-19 outbreak into an IRB-approved research study and sequenced their SARS-CoV-2 infections. We find these viral sequences are identical to each other, but are distinct from over 190,000 publicly available SARS-CoV-2 genomes. These genomes fall as part of a lineage circulating in the USA since April or May 2020 and detected in Virginia and Michigan. Looking forwards, sequencing of additional community SARS-CoV-2 infections collected in the USA prior to October 2020 may shed further light on its geographic ancestry. In sequencing of SARS-CoV-2 infections collected after October 2020, it may be possible to identify infections that likely descend from the White House COVID-19 outbreak.
Development of a Novel Index to Characterise Arterial Dynamics Using Ultrasound Imaging
Authors: Ward Joel , Cheng Xinghao , Xiao Yingyi , Lapolla Pierfrancesco , Chandrashekar Anirudh , Handa Ashok , Cleveland Robin A , Lee Regent 
Published: November 2020

AbstractAbdominal aortic aneurysms (AAA) are associated with systemic inflammation and endothelial dysfunction. We previously reported flow mediated dilatation (FMD) of the brachial artery as a predictor of AAA growth. We hence hypothesised that other physical characteristics of the brachial artery correlate with AAA growth. Using a prospectively cohort of AAA patients, we devised a ‘brachial artery relaxation index’ (BARI) and examined its role as a biomarker for AAA growth. However, no correlation between BARI and future aneurysm growth was observed (p=0.5). Therefore, our investigations did not substantiate the hypothesis that other physical characteristics of the brachial artery predicts AAA growth.
Popularizing recombinant baculovirus-derived OneBac system for scaling-up production of all recombinant adeno-associated virus vector serotypes
Authors: Wu Yang , Han Zengpeng , Duan Mingzhu , Jiang Liangyu , Tian Tiantian , Jin Dingyu , Wang Qitian , Xu Fuqiang 
Published: November 2020

AbstractRecombinant adeno-associated virus (rAAV) has been widely used as an efficient transgenic vector in biomedical research, as well as gene therapy. Serotype-associated transduction efficiency, tissue- or cell-type tropism and immunological profile are major considerations in the various applications of rAAVs. There are increasing needs for different serotypes of rAAV, either naturally isolated or artificially engineered. However, affordable and scalable production of a desired serotype of rAAV remains very difficult, especially for researchers lacking relevant experience. On the basis of our previously established single recombinant baculovirus expression vector (BEV)-derived OneBac system, we have optimized the process and expanded the rAAV production range to the full range of serotypes rAAV1-13. Firstly, the AAVCapgene was optimized to translate by ribosome leaky scanning and the gene of interest (GOI) was cloned into the pFD/Cap-(ITR-GOI)-Rep2 shutte plasmid. Following the classical Bac-to-Bac method, sufficient BEV stock containing all rAAV packaging elements can be quickly obtained. Finally, we can repeatedly scale up production of rAAVs in one week by using a single BEV to infect suspension-cultured Sf9 cells. The rAAV1-13 show relatively high yields ranging from 5×104to 4×105VG/cell. More than 1×1015VG purified rAAVs can be easily obtained from 5 L suspension-cultured Sf9 cells. As expected, rAAV serotypes 1-13 show different potencies forin vitrotransduction and cell-type tropisms. In summary, the single BEV-derived OneBac system should prove popular for laboratory scaling-up production of any serotype of rAAV.
Microvilli-derived Extracellular Vesicles Govern Morphogenesis inDrosophilawing epithelium
Authors: Hurbain Ilse , Macé Anne-Sophie , Romao Maryse , Sengmanivong Lucie , Ruel Laurent , Basto Renata , Thérond Pascal P. , Raposo Graça , D’Angelo Gisela 
Published: November 2020

ABSTRACTThe regulation and coordination of developmental processes involves the secretion of morphogens and membrane carriers, including extracellular vesicles, which facilitate their transport over long distance. The long-range activity of the Hedgehog morphogen is conveyed by extracellular vesicles. However, the site and the molecular basis of their biogenesis remains unknown. By combining fluorescence and electron microscopy combined with genetics and cell biology approaches, we investigated the origin and the cellular mechanisms underlying extracellular vesicle biogenesis, and their contribution to Drosophila wing disc development, exploiting Hedgehog as a long-range morphogen. We show that microvilli of Drosophila wing disc epithelium are the site of generation of small extracellular vesicles that transport Hedgehog across the tissue. This process requires the Prominin-like protein, whose activity, together with interacting cytoskeleton components and lipids, is critical for maintaining microvilli integrity and function in secretion. Our results provide the first evidence that microvilli-derived extracellular vesicles contribute to Hedgehog long-range signaling activity highlighting their physiological significance in tissue developmentin vivo.
Discovery of five HIV nucleoside analog reverse-transcriptase inhibitors (NRTIs) as potent inhibitors against the RNA-dependent RNA polymerase (RdRp) of SARS-CoV and 2019-nCoV
Authors: Sun Jialei 
Published: November 2020

AbstractThe outbreak of SARS in 2002-2003 caused by SARS-CoV, and the pandemic of COVID-19 in 2020 caused by 2019-nCoV (SARS-CoV-2), have threatened human health globally and raised the urgency to develop effective antivirals against the viruses. In this study, we expressed and purified the RNA-dependent RNA polymerase (RdRp) nsp12 of SARS-CoV and developed a primer extension assay for the evaluation of nsp12 activity. We found that nsp12 could efficiently extend single-stranded RNA, while having low activity towards double-stranded RNA. Nsp12 required a catalytic metal (Mg2+or Mn2+) for polymerase activity and the activity was also K+-dependent, while Na+promoted pyrophosphorylation, the reverse process of polymerization. To identify antivirals against nsp12, a competitive assay was developed containing 4 natural rNTPs and a nucleotide analog, and the inhibitory effects of 24 FDA-approved nucleotide analogs were evaluated in their corresponding active triphosphate forms. Ten of the analogs, including 2 HIV NRTIs, could inhibit the RNA extension of nsp12 by more than 40%. The 10 hits were verified which showed dose-dependent inhibition. In addition, the 24 nucleotide analogs were screened on SARS-CoV primase nsp8 which revealed stavudine and remdesivir were specific inhibitors to nsp12. Furthermore, the 2 HIV NRTIs were evaluated on 2019-nCoV nsp12 which showed inhibition as well. Then we expanded the evaluation to all 8 FDA-approved HIV NRTIs and discovered 5 of them, tenofovir, stavudine, abacavir, zidovudine and zalcitabine, could inhibit the RNA extension by nsp12 of SARS-CoV and 2019-nCoV. In conclusion, 5 FDA-approved HIV NRTIs inhibited the RNA extension by nsp12 and were promising candidates for the treatment of SARS and COVID-19.
Identification of compounds enhancingAqp4stop codon readthrough
Authors: Sapkota D. , Dougherty J.D. 
Published: November 2020

AbstractAn unusual stop codon readthrough event generates a conserved C-terminally elongated variant of the water channel protein Aquaporin 4 (AQP4). In the brain, AQP4 is astrocyte-specific, required for normal functioning of the glymphatic system, and involved in the clearance of the Alzheimer’s associated protein Amyloid beta. Further, the readthrough variant is localized exclusively perivascularly, and the perivascular pool of AQP4 is reduced in Alzheimer’s and several other neurological diseases. However, there are currently no means of increasing or restoring the perivascular pool AQP4. Here we identify a compound that can enhanceAqp4stop codon readthrough. We screened 2600 compounds, mostly approved drugs and pharmacologically active natural compounds, using a luciferase reporter system. 28 candidate lead compounds were then subjected to a variety of secondary screening steps using orthogonal reporter systems and characterizing dose-response activities. Finally, we tested the top compounds’ abilities to generate readthrough of the endogenousAqp4transcript, identifying Apigenin as an enhancer of this biological phenomenon. This compound can allow modulation of readthrough in experimental systems, mechanistic studies of programmed readthrough, and suggests the potential for modulating Alzheimer’s disease through pharmacological enhancement of perivascular AQP4.
Epigenetic regulation of epithelial dectin-1 through an IL-33-STAT3 axis in allergic disease
Authors: Yong Hwan Mee , Gour Naina , Sharma Deepika , Khalil Syed Muaz , Lane Andrew P. , Lajoie Stephane 
Published: November 2020

AbstractAllergic diseases arise in susceptible individuals in part because of decrements in protective pathways. The mechanism by which these anti-inflammatory molecules become repressed remains unclear. We have previously reported that epithelial dectin-1 prevents aberrant type 2 responses and is downregulated in the epithelium of allergic patients. Here we report that dectin-1 is constitutively expressed by the respiratory epithelium in humans, and that IL-33 specifically acts as a repressor of dectin-1. Mechanistically, this occurs via IL-33-dependent STAT3 activation and the subsequent repression of the dectin-1 gene, CLEC7A. We have identified a novel enhancer region upstream of the proximal promoter of CLEC7A that is only accessible in epithelial cells, but not in hematopoietic cells. Epigenetic repression of CLEC7A through this newly identified locus, downstream of an aberrant IL-33-STAT3 axis, occurs in the epithelium of allergic individuals. Collectively, our data identify a mechanism of epigenetic fine-tuning of dectin-1 expression in epithelial cells that may participate in allergenicity.
Servant leadership and followers' creativity: does climate for creativity matter?
Authors: Aboramadan Mohammed 
Published: November 2020

PurposeThis research proposes, building on social exchange theory and the componential theory of creativity, a model of servant leadership to investigate its effect on followers' creativity through the intervening mechanism of climate for creativity in the hospitality industry, operating in a non-Western context.Design/methodology/approachThe study predicted that climate for creativity will play a significant intervening role in the servant leadership–creativity relationship. The study’s data were collected from 232 employees working in 70 Palestinian hotels. Data were analyzed using structural equation modeling (SEM) analyses along with techniques used to reduce common method bias.FindingsThe results revealed the significance of climate for creativity as a partial mediator in the relationship between servant leadership and followers' creativity.Practical implicationsThe results might be useful for hotel managers in the context of utilizing servant leadership roles for fostering a creative climate. They might, therefore, consider placing servant leaders as a recruitment agenda priority.Originality/valueThis research is novel in three ways. First, its aim is to enrich the empirical literature on servant leadership, which is still in a maturity stage. Second, even with the research studies that are available, limited analysis is found on how servant leadership can stimulate employees' behaviors in the hospitality industry. Third, the study has been conducted in a non-Western context, in contrast to most servant leadership research studies being carried out in Western countries.
The need for and benefits of buildability analysis: Nigeria as a case study
Authors: Osuizugbo Innocent Chigozie 
Published: November 2020

Purpose
Buildability analysis has a potential of improving the outcomes of construction projects. However, the usage of buildability analysis is not well pronounced within the construction sector. This study aims to provide understanding of the concept of buildability analysis with the view to predicting the future stance for buildability analysis.

Design/methodology/approach
The study adopted a survey research method. Questionnaires were administered to purposively selected group of construction professionals working in Nigeria. A total of 82 questionnaires were administered among the sampled respondents, out of which a total of 51 (62.2%) were adequately filled and returned. The data collected were analysed using descriptive and inferential statistics.

Findings
The results revealed inability of the designers and constructors to have a common upstanding of the entire construction process, fragmented nature of the construction industry and poor correlation between design and construction as the most essential needs for buildability analysis. On the benefits of buildability analysis, the results showed increase in efficiency, save wasteful efforts and quality improvement as the most significant ones. In addition, the results from the study revealed that there is a statistically significant difference in some of the needs for and benefits of buildability analysis among the survey participants.

Originality/value
The study identified three future stances for buildability analysis to include building unity, efficient and effective construction and high competition. Framework for buildability analysis was developed aimed at providing guidance to professionals in achieving enhanced project production information. The findings provide in-depth insight of the needs for and benefits of buildability analysis that can inform professionals in the construction industry to adopt buildability analysis programme as a lasting solution to most design challenges and buildability problems confronting the sector.