bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of Pediococcus Acidilactici J9 in Decreasing the Occurrence of Gastritis Caused by H.pylori Infection and Two-week Repeated-dose Oral Toxicity Study in Mice
Authors: Lee Mijung , Chung Jin-Young , Kim Ka Yeun , Im Wooseok , Kim Manho 
Published: May 2020

Abstract
BACKGROUND Helicobacter pylori (H. pylori) is an important pathogen that causes chronic gastritis and peptic ulcer, and is related to the development of gastric carcinoma. Several chemicals, including antibiotics, have been used to eradicate H.pylori. However, more studies are yet requred to accomplish a sufficient therapy. Pediococcus acidilactici J9 were studied for inhibition of binding of H.pylori binding to human gastric cell lines. This study was performed in order to investigate the repeated-dose toxicity of Pediococcus acidilactici J9 in male and female mice.RESULTS C57BL/6 male and female Mus musculus were divided into four groups (n = 10 in each group). Pediococcus acidilactici J9 was administered daily by oral injection of vehicle control at dosage levels to a low-dose group (500 mg/kg/day), middle-dose group (1000 mg/kg/day), and high-dose group (2000 mg/kg/day) for two weeks. After 14 days of exposure, the blood biochemistry and hematology were investigated, along with a histopathology exam. There were no bacterial-related deaths or abnormal clinical signs in either gender of mouse. The data was observed during the period in terms of body weight, food, intake, and water consumption. Also, no alterations in organ weights upon administration of Pediococcus acidilactici J9 alone were observed.CONCLUSIONS These results suggest that the oral application of Pediococcus acidilactici J9, up to a dosage level of 2,000 mg/kg/day, causes no adverse effects in both male and female mice. Pediococcus acidilactici J9 inhibits the adhesion of H.pylori to AGS gastric cancer cells. When used as probiotics, Pediococcus acidilactici J9 may help decrease the occurrence of gastritis and reduce the risk of H.pylori infection with promising safety issues.
Menstrual blood-derived mesenchymal stem cells provide new insights into the treatment of coronavirus disease 2019 (COVID-19)
Authors: Chen Xin , Yu Liang , Chen Lijun , Zheng Xiaoqin , Tang Lingling , Xu Kaijin , Cai Hongliu , Chen Yu , Zheng Shufa , Lu Juan , Xu Zhenyu , Zhang Qiang , Gao Hainv , Li Yifei , Qu Jingjing , Jiang Yingan , Xu Xiaowei , Xiang Charlie , Li Lanjuan 
Published: May 2020

Abstract
Background: The coronavirus disease 2019 (COVID-19) causing a cluster of respiratory infections in Wuhan, China, is identified in December 2019. The main symptoms are defined as fever, cough, shortness of breath, with early symptom of sputum, acute respiratory distress syndrome (ARDS), and the final lung injury and pulmonary fibrosis. Currently, there is no effective method to cure it. Mesenchymal stem cell (MSC) therapy is an immediate need for treating COVID-19 especially severe patients at present.Methods: We describe the two confirmed case of COVID-19 severe patients in Hangzhou, China to explore the role of menstrual blood-derived MSC in the treatment of SARS-CoV-2 infection. Furthermore, we mimic disease model of pulmonary fibrosis in mice to assess the role of MSC. Then, a co-culture system to investigate the underlying mechanism between MSC and pulmonary-associated cells by a series of Physiological, biochemical, bioinformatics analysis.Results: MSC transplantation increases the immune indicators (including lymphocytes) and decreases inflammatory indicators (such as IL-6, IL-10, TNF, and IFN). More importantly, the two patients alleviated symptom and discharged after 3 weeks’ treatment with MSC. Additionally, MSCs exhibit an anti-inflammatory role through suppressing some inflammatory factors (RANTES, GM-CSF, MIG-1g, MCP-5, Eotaxin), which is anastomotic to current clinical study using MSC to treat COVID-19. Conclusions: This is the first report using menstrual blood-derived MSC in treating COVID-19 patients. From our clinical results, we hold one idea that MSCs reduced inflammatory effect to defend cytokine storm. The underlying mechanism is probably that MSCs inhibit epithelia cell apoptosis and reduce the secretion of inflammatory factors to prevent myofibroblasts activity. MSC provides an alternative method for treating COVID-19 particularly some patients with ARDS or subsequent pulmonary fibrosis.Trial registration: This clinical trial was submitted to and approved by the Ethics Committee of the First Affiliated Hospital, Collage of Medicine, Zhejiang University. MSC administration in patient with COVID-19 was conducted in a single center and open-label clinical trial (ChiCTR2000029606).
Home range expansion in response to temperature by evening bats (Nycticeius humeralis) in an urban environment
Authors: Hall Ellen , Bennett Victoria 
Published: May 2020

Abstract
Background: Despite the negative connotation of urban sprawl for bat populations, fragmented green spaces such as parks, cemeteries, and golf courses have the potential to provide necessary resources for bats. For example, water resources in these areas can include natural or semi-natural lakes, ponds, streams, and drainage ditches. Such water resources, however, are frequently ephemeral when subject to prolonged periods of high temperatures. We, therefore, hypothesize that bats will expand or shift their home ranges from these urban green spaces into the surrounding neighborhoods to access alternative resources, such as residential swimming pools. Methods: To explore whether bats expand their ranges from urban green spaces, we conducted a telemetry study in which we radio-tracked resident evening bats (Nycticeius humeralis) in a local park system during their summer activity period from 2017-2019 in Fort Worth, Texas, USA. From radio-tracking surveys, we measured home range size using a k-LoCoH method and the percentage of these home ranges that fell within the park system. We compared these variables using linear and non-linear regressions with temperature. Results: We successfully tracked a total of 30 bats over the 3-year period and found a positive correlation between home range size and temperature. Furthermore, we observed that home ranges increased 6 times in size when temperatures exceeded 30ºC. Conclusions: Our study indicates the importance of urban neighborhoods surrounding green spaces in providing alternative resources, such as water, for bats. If managed appropriately, these urban areas have the potential to act as urban oases for bat populations, which in turn can contribute to their conservation.
The value of lung involvement in recognizing severe dengue-A retrospective study
Authors: Shi Yunfeng , Gan Weiqiang , Shi Xiaohan , Liang Jingjing , Chen Xiaoxia , Guo Yuefei , Tong Yuwei , Gao Zhiliang , Wu Benquan 
Published: May 2020

Abstract
Background Dengue fever is a vector-borne disease transmitted by infected female Aedes mosquitoes. It has become the major threat to human beings especially in tropical and subtropical areas. It's important to find early parameters to identify severe dengue due to its poor prognosis. This study aimed to explore the value of lung involvement in identifying severe dengue (SD) from ordinary dengue.Methods 592 dengue patients admitted in the Third Affiliated Hospital of Sun Yat-sen University from July 2014 to January 2020 were enrolled. Patients were divided into two groups, dengue with lung involvement group (DWLI) and dengue without lung involvement group (DWOLI). According to the severity of dengue fever, patients in DWLI group were further divided into dengue subgroup and SD subgroup. The clinical characteristics, treatment and outcome of the patients were analyzed between groups and subgroups respectively.Results The rate of SD patients was higher in DWLI group than that in DWOLI group. The ratios of old age, smoking, hepatobiliary disease, hypertension, diabetes, coronary heart disease and cerebrovascular disease were higher in DWLI group than those in DWOLI group (all p<0.05). Other organs such as liver, kidney, digestive tract were affected more in DWLI group (all p<0.05). Respiratory symptoms such as cough and breathlessness were more common in DWLI group (both p<0.05). Subgroup analyses showed that SD subgroup patients had more respiratory symptoms as well (p<0.05). Inflammatory markers including C-reactive protein (CRP) and pro-calcitonin (PCT) were increased in DWLI group (both p<0.05). The ratios of pleural effusion and bilateral lung infiltration were correlated with SD (both p<0.05). The ratios of anti-virus，anti-bacteria and combined therapy were higher in DWLI group. There were 10 cases in DWLI group complicated with multiple organ dysfunction syndrome (MODS) while no MODS occurred in DWOLI group. Except for 2 patients with MODS died, all the other patients were cured.Conclusions Among dengue fever patients, the elderly, smoking population, those accompanied by underlying diseases were prone to have lung involvement. Dengue fever patients who develop clinical manifestations such as cough, breathlessness, pleural effusion and bilateral pneumonic exudation should be warned of SD.
Foraging and commuting habitats of the greater horseshoe bat, revealed by high-resolution GPS-tracking
Authors: Fujioka Emyo , Yoshimura Koki , Ujino Tomohiro , Yoda Ken , Fukui Dai , Hiryu Shizuko 
Published: May 2020

Abstract
Background Echolocating bats make a series of decisions to select their flight routes based on auditory information obtained by sonar; accumulations of these flight routes are represented as daily movement patterns. However, there is still a lack of a unified understanding of continuous movements of echolocating bats in the wild from small to large spatial scales (i.e., from meters to tens of kilometers). In this study, we investigated nightly flight paths of the Japanese greater horseshoe bat, Rhinolophus ferrumequinum nippon, using high-resolution GPS data loggers. Our aim was to identify foraging and commuting behavior based on the observed movement patterns and to investigate the relationship between these movement types and specific habitats.Results We found that the majority of tagged bats alternated between foraging and commuting behavior throughout the tracking period, and one individual moved 23.6 km from its roost. The bats usually left a stay point in the opposite direction from which they entered it, indicating that almost all of the stay sites were on their way to another destination. The distance between two successive foraging sites was on average 332 ± 398 m (mean ± standard deviation), and half of all foraging periods lasted less than 3 min. An analysis of habitat use revealed that the bats used conifer plantation and natural forests as pathways and feeding habitats.Conclusions Our findings suggest that the structure of the forests have a great influence on the nocturnal behavior of the greater horseshoe bats. Although this study has a descriptive character due to a relatively small number of tagged individuals, it was possible to elucidate the small-scale interactions between wild Rhinolophus bats and their environment using the latest high-resolution GPS technology, which will allow us to give new insights into the foraging ecology of echolocating bats in the wild.
Analyzing the determinants of the spread of covid-19 among the provincial regions in China
Authors: Shen Jianfa 
Published: May 2020

Abstract
China has experienced the spatial diffusion of covid-19 from Wuhan since December 2019. This research examines the relationship between the geographical, social and economic factors and the number of covid-19 cases on 26 January, 7 February, 20 February and 6 March 2020 in mainland China. Both correlation and regression analyses show that the migrants who moved into Hubei in 2005-2010 is a good indicator of the population flow from Hubei to other provinces that caused the spread of covid-19 in early 2020. Many migrants travelled back to hometown just before the traffic ban of Wuhan city for the spring holiday. Thus the migration flow from a province to Hubei in the period 2005-2010 had the highest correlation coefficient with the number of covid-19 cases in four selected dates. The population flow data from the Baidu map on 20 January 2020 were also highly correlated with the number of cases, but not as good as above migration data. The regression equation for the number of cases on 26 January 2020 had the highest adj R2 as it was mainly determined by the population flow from Hubei. The numbers of cases in subsequent days were also affected by the local diffusion and the control measure in various provinces. Income and economic variables became additional explanatory variables indicating their complicated impacts on the mitigation measures at various provinces in China. The results of this research have important policy implications to respond to the covid-19 pandemic.
WITHDRAWN: Lysenin toxin insertion mechanism is Calcium-dependent
Authors: Munguira Ignacio L.B. 
Published: May 2020

Abstract
Pore Forming Toxins (PFTs), formed mainly by virulence factors of bacteria, belongs to Pore Forming Protein (PFP) family. Secreted as soluble monomers, they bind specific targets in membranes where their oligomerization and insertion place. Lysenin, a member of the PFTs, forms and oligomer after sphingomyelin binding, the so-called prepore, which become inserted forming a pore after a conformational change triggered by a pH decrease. In crowded conditions, oligomers tends to stay in prepore form because the prepore-to-pore transition is sterically blocked. In this study, we investigate the effect of calcium ions in those crowded conditions, finding that calcium act as a trigger for lysenin insertion. We localize the residues responsible for calcium sensitivity in a small α-helix. Our results are not only one of the few complete structural descriptions of prepore-to-pore transitions but the very first that involves a calcium triggering mechanism. The presence of glutamic or aspartic acids in the insertion domains could be an indication that calcium may be a general trigger for PFTs and more generally PFP.
Drug repurposing and polypharmacology to fight SARS-CoV-2 through the inhibition of the main protease
Authors: Pinzi Luca , Tinivella Annachiara , Caporuscio Fabiana , Rastelli Giulio 
Published: May 2020

Abstract
There is an urgent need to develop therapeutic options to fight the outbreak of a novel Coronavirus (SARS-CoV-2), which causes a disease named COVID-19 and is spreading rapidly around the world. Drug repurposing can significantly accelerate the identification of drug candidates suitable for clinical evaluation. Moreover, drugs with polypharmacological effects may increase antiviral activity and/or counteract severe disease complications concurrently affecting COVID-19 patients. Herein, we present the results of a computational drug repurposing campaign in search for potential inhibitors of the main protease of SARS-CoV-2. To this aim, the complete DrugBank database, including drug metabolites, was docked to the recently solved crystal structure of the SARS-CoV-2 Mpro and the results were post-processed by using our in-house tool BEAR. Here we report 32 promising drugs that could be repositioned to fight SARS-CoV-2. Some of them have already entered clinical trials against COVID-19, thus supporting our results, but the vast majority of the selected compounds is new and has never been considered before. For each repurposed compound its therapeutic relevance and the potential beneficial polypharmacological effects that may arise thanks to its original therapeutic indication are thoroughly discussed.
A RESPONSABILIDADE DO FRANQUEADOR PELOS DÉBITOS TRABALHISTAS DO FRANQUEADO
Authors: PELEGRINI Pedro 
Published: May 2020

O âmago do presente estudo é aclarar a possibilidade de responsabilização do franqueador por débitos trabalhistas de seu franqueado. O instituto da franquia se faz cada vez mais presente na rotina dos brasileiros, devido a sua grande seguridade institucional, bem como a sua alta rentabilidade. Apesar de ser modelo de renome entre os moldes de negócios, o instituto da franchising carrega consigo algumas dúvidas. A eventualidade de existir compromisso do franqueador adimplir débitos trabalhistas do franqueado é uma dessas questões, pois se essa for deflagrada, o fascínio pelo retro citado instituto pode vir a diminuir. Serão levantadas ao longo deste exame, três probabilidades da referida responsabilização. Duas delas, envolvendo outras figuras jurídicas consolidadas e sua aplicação e enquadramento ao instituto da franquia, à saber, terceirização e grupo econômico; bem como uma probabilidade doutrinária e social, a situação de hipossuficiência do trabalhador mediante o seu empregador. Ao fim, buscar-se-á esmiuçar a incidência dos os institutos, respeitando o ordenamento jurídico e o egrégio entendimento, sem penalizar a efígie da franquia. Perscrutaremos demonstrar que no desenvolvimento perfeito da franchising, não há como haver a questionada responsabilização, isto é, na incidência exata do disposto ao contrato de franquia, a obrigação de arcar com eventuais débitos trabalhistas do franqueado, está completamente afastada do franqueador. Para que tal relação obrigacional ganhe subsistência, carece de mutação nos moldes da franquia, ou seja, deturpação no que fora previamente alinhavado.
Magnetite texture and trace-element geochemistry fingerprint of pulsed mineralization in the Xinqiao Cu-Fe-Au deposit, Eastern China
Authors: Zhang Yu , Hollings Pete , Shao Yongjun , Li Dengfeng , Chen Huayong , Li Hongbin 
Published: May 2020

Abstract
The origin of stratabound deposits in the Middle-Lower Yangtze River Valley Metallogenic Belt (MLYRB), Eastern China, is the subject of considerable debate. The Xinqiao Cu-Fe-Au deposit in the Tongling ore district is a typical stratabound ore body characterized by multi-stage magnetite. A total of six generations of magnetite have been identified. Mt1 is commonly replaced by porous Mt2, and both are commonly trapped in the core of Mt3, which is characterized by both core-rim textures and oscillatory zoning. Porous Mt4 commonly truncates the oscillatory zoning of Mt3, and Mt5 is characterized by 120° triple junction texture. Mt1 to Mt5 are commonly replaced by pyrite that coexists with quartz, whereas Mt6, with a fine-grained foliated and needle-like texture, commonly cuts the early pyrite as veins and is replaced by pyrite that coexists with calcite. The geochemistry of the magnetite suggests that they are hydrothermal in origin. The microporosity of Mt2 and Mt4 magnetite, their sharp contacts with Mt1 and Mt3, and lower trace-element contents (e.g., Si, Ca, Mg, and Ti) than Mt1 and Mt3 suggest that they formed via coupled dissolution and reprecipitation of the precursor Mt1 and Mt3 magnetite, respectively. This was likely caused by high-salinity fluids derived from intensive water-rock interaction between the magmatic-hydrothermal fluids associated with the Jitou stock and Late Permian metalliferous black shales. The 120° triple junction texture of Mt5 suggests it is the result of fluid-assisted recrystallization, whereas Mt6 formed by replacement of hematite as a result of fracturing. The geochemistry of the magnetite suggests that the temperature increased from Mt2 to Mt3 and implies that there were multiple pulses of fluids from a magmatic-hydrothermal system. Therefore, we propose that the Xinqiao stratiform mineralization was genetically associated with multiple influxes of magmatic hydrothermal fluids derived from the Early Cretaceous Jitou stock. This study demonstrates that detailed texture examination and in situ trace-elements analysis under robust geological and petrographic frameworks can effectively constrain the mineralization processes and ore genesis.
Исследование параметров внутренней картины болезни пациентов ревматологического профиля
Authors: Ялтонский Владимир М. , Абросимов Илья Н. , Андрушкевич Тамара Д. , Шашурина Елизавета М. 
Published: May 2020

Introduction. A patient’s subjective pattern of disease (SPD) is considered both as a result of his/her adjustment to disease and as an instrument of personal self-regulation in disease. Modern clinical psychology actively investigates this psychological construct, which expands available knowledge obtained in the process of determining psychological factors of the treatment process. The present study appears to be the first that examines rheumatoid patients using a SPD multilevel model, which focuses on illness perceptions and coping with it. Methods. The study sample consisted of 80 patients with rheumatic diseases aged 18–52 years, 50 % of whom were patients with rheumatoid arthritis (RA) and 50 % of whom were patients with systemic lupus erythematosus (SLE). The study used the following techniques for assessing the structure and content of various levels of the subjective pattern of disease: (a) the Symptoms Check-List-90-Revised (SCL-90-R), (b) the Brief Inventory of Illness Perceptions, and (c) the technique for psychological assessment of coping with stressful and problematic events. Results. In the comparison groups, significant differences were observed on the scales of illness perceptions (‘illness understanding’, ‘course of illness’, ‘control of illness’, ‘control of treatment’), the scales of coping behavior (‘search for social support’, ‘acceptance of responsibility’), and the scales of ‘somatization’, ‘depression’, and ‘anxiety’. The correlations indicate associations among the parameters of various levels of the subjective pattern of disease in patients in both groups. Discussion. The parameters of the subjective pattern of disease in patients with systemic lupus erythematosus indicate their desire to recognize and overcome uncertainty and unpredictability of their disease. However, this does not reduce their emotional involvement in the problem. The subjective pattern of disease in patients with rheumatoid arthritis is characterized by a worse understanding and control of disease, as well as by emotion-focused coping strategies aimed at distancing from anxiety-related experiences associated with their disease.