bluuebunny/crossref_metadata_2025_split huggingface dataset:
Site of Relapse of the Ductal Adenocarcinoma of the Pancreas affects Survival after Multimodal Therapy
Authors: Safi Sami Alexander , Lehwald Nadja , Rehders Alexander , Haeberle Lena Julia , Keitel Verena , Knoefel Wolfram Trudo 
Published: July 2020

Abstract
Background
The ductal adenocarcinoma of the pancreas (PDAC) remains one of the most lethal malignancies. To date, no guidelines exists for isolated resectable metachronous disease. It is still unclear, which patients may benefit from relapse surgery. The aim of our study was to compare disease free survival (DFS) and relapse specific survival (RSS) in patients with isolated local recurrence, metachronous hepatic or pulmonary metastases.
Methods
Patients with isolated resectable metachronous disease of either local recurrence, hepatic or pulmonary metastases were included for survival analyses. RSS of surgically treated patients (local (n = 11), hepatic (n = 6) and pulmonary metastases (n = 11)) were compared to conservative treated patients (local (n = 20), hepatic (n = 49) and pulmonary metastases (n = 14)).
Results
Patients with isolated metachronous hepatic metastases showed a higher T-stage and hepatic M-stage in the primary staging of the previous resected PDAC when compared to the other patients. DFS before diagnosed pulmonary metastases was longer compared to DFS before diagnosed hepatic metastases or local recurrence. Surgical resection only significantly improved RSS in patients with local recurrence or pulmonary metastases, when compared to patients after chemotherapy. Long-term survivors (> 5 years) were only detected after surgery for isolated local recurrence and 45% of these patients were still alive in our study.
Conclusion
Although DFS before diagnosed isolated local recurrence was dismal and comparable to patients with isolated hepatic metastases, long-term survivors were only detected in this group, recommending a surgical approach if resectability is provided.
The relationship between time to surgery(TTS) after neoadjuvant chemotherapy (NAC)and survival in breast cancer patients: meta-analysis
Authors: Su YongCheng , Zheng XiaoGang 
Published: July 2020

Abstract
PURPOSE: This meta-analysis aims to evaluate the impact of delaying surgery in operable breast tumor patients after neoadjuvant chemotherapy (NAC) on survival.METHODS: An electronic literature retrieval was conducted on PubMed/Medline and EMBASE((between January 2000 and June 2020).The primary end point was overall survival(OS),secondary end points included disease-free survival (DFS) or recurrence-free survival (RFS).The HR with 95% confidence intervals were calculated using a random-effects or fixed-effects model.RESULTS: The combined HR for OS was 1.52(95% CI 1.29-1.78; P = 0.000) by fixed-effects model.No statistically significant heterogeneity was found (P=0.114;I2=39.8%).The pooled HR for RFS/DFS was 1.47 (95%CI: 1.27- 1.71,I2=61.9%) by random-effects model, with significant heterogeneity.CONCLUSION: Our meta-analysis revealed a significant adverse association between longer TTS after NAC and more inferior OS and RFS/DFS in patients with breast cancer.Clinicians and patients should minimize surgical delay after NAC as much as possible.
Feasibility of a quality improvement project with an interrupted time series design to increase adherence to evidence-based pulmonary embolism diagnosis in the emergency department.
Authors: Germini Federico , Hu Yang , Afzal Sarah , Niaz Saghar , Chan Teresa , Clayton Natasha , Mondoux Shawn , Thabane Lehana , de Wit Kerstin 
Published: July 2020

Abstract
Background: Many evidence-based clinical decision tools are available for the diagnosis of pulmonary embolism (PE). However, these clinical decision tools have had suboptimal uptake in the everyday clinical practice in emergency departments (EDs), despite numerous implementation efforts. We aimed to test the feasibility of a multi-faceted intervention to implement an evidence-based PE diagnosis protocol.Methods: We conducted an interrupted time series study in three EDs in Ontario, Canada. We enrolled consecutive adult patients accessing the ED with suspected PE from January 1, 2018 to February 28, 2020. Components of the intervention were: clinical leadership endorsement; a new pathway for PE testing; physician education; personalized confidential physician feedback, and collection of patient outcome information. The intervention was implemented in November 2019. We identified six criteria for defining the feasibility outcome: successful implementation of the intervention in at least two of the three sites; capturing data on ≥ 80% of all CTPAs ordered in the EDs; timely access to electronic data; rapid manual data extraction with feedback preparation before the end of the month ≥ 80% of the time; time required for manual data extraction and feedback preparation ≤ 2 day per week in total.Results: The intervention was successfully implemented in two out of three sites. 5,094 and 899 patients were tested for PE in the period before and after the intervention, respectively. We captured data from 90% of CTPAs ordered in the EDs, and we accessed the required electronic data. The manual data extraction and individual emergency physician audit and feedback were consistently finalized before the end of each month. The time required for manual data extraction and feedback preparation was ≤ 2 days per week (14 hours).Conclusions: We proved the feasibility of implementing an evidence-based PE diagnosis protocol in two EDs. We were not successful implementing the protocol in the third ED.Registration: The study was not registered.
The Allele Frequency Variation of Autosomal 19 Short Tandem Repeat (STR) Loci in Drug Abuser:&nbsp;A Pilot Study
Authors: LIN xia , ZHANG xiaoyan , SU qin , Jin Bo 
Published: July 2020

Abstract
Backgrounds: Substance use disorders (SUDs) refers to drug abuse of 10 separate classes impacting seriously mental health.Objective: This study aims to explore the genetic polymorphism of the 19 short tandem repeat (STR) loci (D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, VWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338, and FGA) and the risk genetic factors of substance use disorders(SUDs).Methods: The capillary electrophoresis was applied to analyze 19 STR loci in 156 drug abusers. The data of 6,134 healthy people in a previous study were applied to contrast as the control group. The differences in the frequency of the gene were analyzed by Chi-square test. Results: The gene distributions of 19 STR in drug addicts were consistent with hereditary rules. The frequency of vWA, FGA, Penta E was significantly different between the drug abuser and the control group (P<0.05).Conclusion: The 19 STR loci might provide highly informative polymorphic data for addicted genetic studies. Furthermore, there might be a predisposing gene for the drug abuser.
Early clearance of serum HE4 and CA125 in predicting platinum sensitive and prognosis in epithelial ovarian cancer
Authors: Rong Yan , Li Li 
Published: July 2020

Abstract
Objectives: To assess the clinical value of early clearance of HE4 and CA125 for platinum sensitive and prognosis in patients with ovarian cancerMethod: HE4 and CA125 value including clinical data of 89 patients with ovarian cancer were collected. The clearance of HE4 and CA125 were assessed base on the platinum sensitivity, two-year PFS, PFS and OS.Results: 16 patients were classified as platinum resistant and 73 as platinum sensitive according to the response to platinum-base chemotherapy. when HE4 clearance after 3rd cycle chemotherapy or CA125 clearance after 1st cycle chemotherapy, it gave the highest AUC of 0.788, with 100% of sensitivity and 57.5% of specificity respectively between platinum resistant and platinum sensitive group. In addition, 59 patients were classified as two-year PFS group and 30 as not achieved two-year PFS group according to obtaining two-year PFS or not. It gave the highest AUC of 0.730, with 83.3% of sensitivity and 62.7% of specificity respectively when HE4 clearance after 3rd cycle chemotherapy or CA125 clearance after 1st cycle. The prolonged PFS and OS were significantly associated by the clearance of HE4 after 3rd cycle chemotherapy (p < 0.0001, p < 0.0001) as well as CA125 after 1st cycle chemotherapy (p < 0.0001, p < 0.0001).Conclusions: Our data suggest that the early clearance of HE4 and CA125 could predict platinum response and prognosis in patients with ovarian cancer. Monitoring the HE4 and CA125 during first-line chemotherapy might be helpful in predicting platinum sensitive and risk to progress and relapse.
Effect of increased intake of milk replacer in young calves on growth and the neonatal mode of action of IGF-I.
Authors: Matsuzaki Masatoshi , Kamiya Mitsuru , Darroch Craig Stuart , Orito Hideki , Kamiya Yuko , Nakamura Yoshi-Nori , Tsuneishi Eisaku 
Published: July 2020

Abstract
Background Optimizing growth and rumen development of calves in the preweaning period can lead to reduced costs of raising replacement heifers, bulls and fattening beef cattle. In the neonatal period, the quantity and quality of milk replacer consumed by calves directly impacts their growth, development and maturational changes in the somatotropic axis. This study evaluated the effects of plane of nutrition in neonatal calves on growth, development of the somatotropic axis and mode of action of IGF-I. Eight Holstein bull calves (3–4 days old) were fed either a low (LM, average 0.63 kg DM/d) or high (HM, average 1.15 kg DM/d) level of a 28% crude protein milk replacer until slaughtered at 41–42 days old.Results Compared to LM calves, calves on the HM diet had heavier (P < 0.01) body, carcass and semitendinosus muscle weights at slaughter. At three weeks of age, plasma insulin-like growth factor I (IGF-I) concentrations rose more rapidly in HM calves and were significantly higher at three, four and six weeks of age compared to LM calves. IGF-I mRNA levels were higher (P < 0.01) in liver, and lower (P < 0.01) in longissimus dorsi muscle in HM calves compared to LM calves. Growth hormone and IGF-I receptor mRNA levels in liver and muscle were not affected by plane of nutrition.Conclusions Increasing intake of a high-protein milk replacer in suckling calves improved growth rate in the preweaning period. The higher plane of nutrition promoted a shift towards an endocrine mode of IGF-I action and a decline in the autocrine/paracrine IGF-I control of muscle growth.
Intervene effect on Streptococcus suis biofilm formation of emodin extracted from Rheum officinale Baill
Authors: Tang Yang , Bai Jingwen , Yang Yu , Bai Xuedong , Bello-Onaghise God’spower , Xu Yaqin , Li Yanhua 
Published: July 2020

Abstract
Background

Streptococcus suis is a zoonotic pathogen that causes serious systemic infections in pigs and humans. It is a great threat to pig breeding and public health safety. The formation of biofilm was one of the main reasons that it’s difficult to cure. Rhubarb water extract can inhibit the formation of biofilm of Streptococcus suis, but the main functional ingredient was not clear. And what’s the potential mechanism of emodin intervene the biofilm formation? Importantly, the gene expression of luxS of Streptococcus suis was significantly decreased with emodin treatment, if there is a possibility that emodin could combine with LuxS protein which in turn inhibit the formation of biofilm?
Methods

Optimization of green ultrasonic extraction of emodin from R. officinale by Response Surface Methodology. The activity of antibacterial was evaluated by MIC assay. And the ability that intervene biofilm formation was evaluated through Crystal violet staining and SEM. The combination mode between emodin and LuxS protein was identified through molecular docking.
Results

The optimum extraction conditions (ethanol concentration of 80%, extraction time of 21 min, and liquid to solid ratio of 12.5:1 mL/g) were determined through the interaction analysis of different influencing factors and model verification experiment. The minimal inhibitory concentration (MIC) of RUEP against S.suis was 15.625 µg·mL-1, and sub-inhibitory concentrations of RUEP (1/2, 1/4, 1/8 and 1/16 MIC) significantly inhibited the biofilm formation of S. suis. Moreover, scanning electron microscopy analysis confirmed that RUEP can damage the layered colony community structure in biofilm and effectively intervene in the biofilm formation of Streptococcus suis. Through molecular docking analyzed, the inhibition of biofilm formation could be realized through the formation of hydrogen bond with residues His14 and Glu 60, π-π staking with His61、Phe80 and hydrophobic interaction with Cys127.
Conclusion

Through our study, we found emodin have good antibacterial and antibiofilm formation properties and the potential mechanism of emodin intervention on biofilm formation of Streptococcus suis was inferred.
The Association between Self-reported Osteoporosis and Chinese Medicine-Constitution Questionnaire – A Cross-Sectional Taiwan Biobank Study
Authors: Yang Hsien-Hui , Chen Chih-Sheng , Lo Hsin-Yi , Ho Chien-Yi , Kao Chia-Hung , Ho Tin-Yun , Yang Tse-Yen 
Published: July 2020

Abstract
Background: Constitutional medicine was as an example of earlier medicine evidence of Chinese Medicine (CM). CM-Constitutional Questionnaire (CM-CQ) commonly considered as the observed various phenotypes as phenome. However, the comprehensive associations between self-reported disease outcomes and questionnaires of CM-CQ remained uncertain. Methods: Taiwan Biobank has begun the collection of these cancer-free volunteers, aged 30-70 with these questionnaires, self-reported disease history, clinical examinations, and genetic information from 2012 as baseline enrollment. This community-, cross-sectional study was conducted by part of Taiwan Biobank and compared the associations among CM-CQ, which reflect these constitutional variables as individual markers.Results: The present study conducted by using 1,998 volunteers (2 withdraw) from Taiwan Biobank. Which separately assessed the associations among 32 diseases and 44 items of CM-CQ questions using the binomial logistic regression model. There were 6 CM-CQ questions with a significant association of self-reported osteoporosis in all CM-CQ. Interestingly, we found that these 6 CM-CQ questions shown a significant association with osteoporosis, even which adjusted with false discovery rate (adjusted p <0.05). The Q1, Q22, and Q37 demonstrated with considerably adjusted p-value and the other CM-CQ, which involved in Q14, Q16, and Q21 shown borderline relation to self-reported osteoporosis, which was existed less residual effect with smoking and drinking habits. Conclusion: This study generated real-world evidence of national biobank for clarified associations between clinical symptom and self-reported diseases. The exploration of self-reported disease-related CM-CQ for further companion diagnostic indicators via comprehensive community-based Taiwan Biobank study should be a possibility.
Physical Fitness and Cardiometabolic Risk in Seven to Nine Year Old&nbsp;Children
Authors: Weisstaub Gerardo , Gonzalez Maria Angelica , Hermoso Antonio Garcia , Salazar Gabriela 
Published: July 2020

Abstract
Background

Obesity and lower physical fitness levels in children are related to the development of cardiometabolic risk. The objective of the study was to determine the relationship of cardio-metabolic risk and physical fitness in school children from Santiago de Chile.
Methods

Physical fitness was assessed as cardiorespiratory fitness and muscle strength. Fitness was measured using the six-minute-walk-test and muscle strength was assessed by hand grip and standing lung jump. Overweight (≥ 1 SD) and obesity (≥ 2 SD) were determined by body mass index. A cross sectional study was done in 452 children (185 boys and 267 girls), age range (7–9 years). Cardio-metabolic-risk (serum glucose, triglycerides, HDL, insulin) and waist for height ratio was expressed as z score. Data was analyzed using bivariate analysis and multiple and logistic regression.
Results

A 21% were obese and 27% overweight. Children with high body fat and low cardiorespiratory fitness and muscle strength had an OR of 6.8-fold (IC 95% 3.4 to 13.9) cardiometabolic risk z-score, compared to those most fit.
Conclusion

School children with higher body fat and lower physical fitness had increased cardio-metabolic-risk-score. No significant difference in cardio-metabolic risk in the non-obese children was found independent of their physical fitness.
Using Near-Infrared Reflectance Spectroscopy (NIRS) to Predict Glucobrassicin Concentrations in Cabbage and Brussels Sprout Leaf Tissue
Authors: Renner Ilse , Fritz Vincent 
Published: July 2020

Abstract
BackgroundGlucobrassicin (GBS) and its hydrolysis product indole-3-carbinol are important nutritional constituents implicated in cancer chemoprevention. Dietary consumption of vegetables sources of GBS, such as cabbage and Brussels sprouts, is linked to tumor suppression, carcinogen excretion, and cancer-risk reduction. High-performance liquid-chromatography (HPLC) is the current standard GBS identification method, and quantification is based on UV-light absorption in comparison to known standards or via mass spectrometry. These analytical techniques require expensive equipment, trained laboratory personnel, hazardous chemicals, and they are labor intensive. A rapid, nondestructive, inexpensive quantification method is needed to accelerate the adoption of GBS-enhancing production systems. Such an analytical method would allow producers to quantify the quality of their products and give plant breeders a high-throughput phenotyping tool to increase the scale of their breeding programs for high GBS-accumulating varieties. Near-infrared reflectance spectroscopy (NIRS) paired with partial least squares regression (PLSR) could be a useful tool to develop such a method. ResultsHere we demonstrate that GBS concentrations of freeze-dried tissue from a wide variety of cabbage and Brussels sprouts can be predicted using partial least squares regression from NIRS data generated from wavelengths between 950 and 1650 nm. Cross-validation models had R2=0.75 with RPD=2.3 for predicting µmol GBS·100g-1 fresh weight and R2=0.80 with RPD=2.4 for predicting µmol GBS·g-1 dry weight. Inspections of equation loadings suggest the molecular associations used in modeling may be due to first overtones from O-H stretching and/or N-H stretching of amines. ConclusionsA calibration model suitable for screening GBS concentration of freeze-dried leaf tissue using NIRS-generated data paired with PLSR can be created for cabbage and Brussels sprouts. Optimal NIRS wavelength ranges for calibration remain an open question.
Racial Differences in Lifestyle, Demographic, and Health Factors Associated with Quality of Life (QoL) in Midlife Women
Authors: Smith Brandi Patrice , Cardoso-Mendoza Esmeralda , Flaws Jodi A. , Madak-Erdogan Zeynep , Smith Rebecca Lee 
Published: July 2020

Abstract
Background
Quality of Life (QoL) is a major indicator of an individual’s overall health. Only a few studies have examined the racial differences between QoL, and risk factors associated with health, demographics, and lifestyle in midlife women. Thus, the purpose of our study is to determine racial differences in QoL in menopausal women due to lifestyle, demographic, and health related factors.
Methods
A stratified ordinal logistic regression model was applied to self-reported questionnaire data from the Midlife Women's Health Study (MWHS) to determine risk factors associated with QoL differences between white and black women during the menopausal transition.
Results
In multivariate models, our results showed black women who had 3 or 4 comorbidities (moderate) were about 4 times as likely to have higher QoL compared to women who had 0 to 2 comorbidities (95% CI: 1.65,10.78). However, the number of comorbidities was not significantly associated with QoL in white women in univariate or multivariate analysis. Further, BMI, education, and income were not significant factors in QoL in black women but were in white women.
Conclusions
Overall, our results illustrate that differences in health, demographic, and lifestyle factors are associated with QoL during menopause. Also, we suggest that future studies evaluate stratified models between racial groups to determine race-specific risk factors related to quality of life.
Shifts in the bacterial community of supragingival plaque related to metabolic associated fatty liver disease
Authors: Zhao Fen , Dong Ting , Yuan Ke-Yong , Wang Ning-Jian , Xia Fang-Zhen , Liu Di , Wang Zhi-Min , Ma Rui , Lu Ying-Li , Huang Zheng-Wei 
Published: July 2020

Abstract
Background

Metabolic-associated fatty liver disease (MAFLD), also known as the hepatic manifestation of metabolic disorders, has become one of the most common chronic liver diseases worldwide. The associations between some oral resident microbes and MAFLD have been described. However, changes to the oral microbial community in patients with MAFLD remain unknown.
Methods

In this study, variations to the supragingival microbiota of MAFLD patients were identified. The microbial genetic profile of supragingival plaque samples from 24 MAFLD patients and 22 healthy participants were analyzed by 16S rDNA sequencing and bioinformatics analysis. Clinical variables, including indicators of insulin resistance, obesity, blood lipids, and hepatocellular damage, were evaluated with laboratory tests and physical examinations.
Results

The results showed that the diversity of the supragingival microbiota in MAFLD patients was significantly higher than that in healthy individuals. Weighted UniFrac principal coordinates analysis and partial least squares discriminant analysis showed that the samples from the MAFLD and control groups formed separate clusters (Adonis, P = 0.0120). There were 27 taxa with differential distributions (linear discriminant analysis, LDA༞2.0) between two groups, among which Actinomyces and Prevotella 2 spp. were over-represented in the MAFLD group with highest LDA score, while Neisseria and Bergeyella spp. were more abundant in the control group. Co-occurrence networks of the top 50 abundant genera in the two groups suggested that the inter-genera relationships were also altered in the supragingival plaque of MAFLD patients. In addition, as risk factors for the development of MAFLD, insulin resistance was positively correlated with the abundances of Granulicatella, Veillonella, Streptococcus, and Scardovia spp., while obesity was positively correlated to the abundances of Streptococcus, Oslenella, Scardovia, and Selenomonas spp. Metagenomic predictions based on Phylogenetic Investigation of Communities by Reconstruction of Unobserved States revealed that pathways related to sugar (mainly free sugar) metabolism were enriched in the supragingival plaque of the MAFLD group.
Conclusions

In conclusion, as compared to healthy individuals, component and interactional dysbioses were observed in the supragingival microbiota of the MAFLD group, which could be further explored as potential oral biomarkers of the MAFLD.