bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinguishing lupus lymphadenitis from Kikuchi disease based on clinicopathological features and C4d immunohistochemistry
Authors: Yu Shan-Chi , Chang Kung-Chao , Wang Hsuan , Li Meng-Fang , Yang Tsung-Lin , Chen Chun-Nan , Chen Chih-Jung , Chen Ko-Chin , Shen Chieh-Yu , Kuo Po-Yen , Lin Long-Wei , Lin Yueh-Min , Lin Wei-Chou 
Published: July 2020

Abstract

Objectives
Distinguishing Kikuchi disease (KD) from lupus lymphadenitis (LL) histologically is nearly impossible. We applied C4d immunohistochemical (IHC) stain to develop diagnostic tools.

Methods
We retrospectively investigated clinicopathological features and C4d IHC staining in an LL-enriched development cohort (19 LL and 81 KD specimens), proposed risk stratification criteria and trained machine learning models, and validated them in an external cohort (2 LL and 55 KD specimens).

Results
Clinically, we observed that LL was associated with an older average age (33 vs 25 years; P=0.005), higher proportion of biopsy sites other than the neck [4/19 (21%) vs 1/81 (1%); P=0.004], and higher proportion of generalized lymphadenopathy compared with KD [9/16 (56%) vs 7/31 (23%); P=0.028]. Histologically, LL involved a larger tissue area than KD did (P=0.006). LL specimens exhibited more frequent interfollicular pattern [5/19 (26%) vs 3/81 (4%); P=0.001] and plasma cell infiltrates (P=0.002), and less frequent histiocytic infiltrates in the necrotic area (P=0.030). Xanthomatous infiltrates were noted in 6/19 (32%) LL specimens. Immunohistochemically, C4d endothelial staining in the necrotic area [11/17 (65%) vs 2/62 (3%); P&lt;10−7], and capillaries/venules [5/19 (26%) vs 7/81 (9%); P=0.048] and trabecular/hilar vessels [11/18 (61%) vs 8/81 (10%); P&lt;10−4] in the viable area was more common in LL. During validation, both the risk stratification criteria and machine learning models were superior to conventional histological criteria.

Conclusions
Integrating clinicopathological and C4d findings could distinguish LL from KD.
Possible misclassification of cardiovascular risk by SCORE in antisynthetase syndrome: results of the pilot multicenter study RI.CAR.D.A
Authors: Triantafyllias Konstantinos , Cavagna Lorenzo , Klonowski Anna , Drott Ulrich , Fiehn Christoph , Wendel Sarah , Bergner Raoul , de Blasi Michele , Voll Reinhard E , Baulmann Johannes , Konstantinides Stavros , Galle Peter R , Schwarting Andreas 
Published: July 2020

Abstract

Objectives
To test the ability of an established traditional cardiovascular (CV) risk prediction score [Systematic COronary Risk Evaluation (SCORE)] and its EULAR modified version (mSCORE) to identify antisynthetase syndrome (ASyS) patients at high CV risk and to examine for the first time associations of CV and cerebrovascular surrogate markers with clinical and immunological ASyS parameters.

Methods
SCORE/mSCORE and the gold standard marker of aortic stiffness [carotid-femoral pulse wave velocity (cfPWV)] were examined in ASyS patients and healthy controls. Moreover, sonography of the common- (CCA) and internal- (ICA) carotid arteries was performed in subsets of both groups, evaluating carotid intima-media thickness (cIMT), plaques and Doppler sonographic cerebrovascular surrogates [resistance (RI) and pulsatility (PI) indices].

Results
We recruited 66 ASyS patients and 88 controls. According to mSCORE, 10% of the patients had high CV risk. However, cfPWV and carotid sonography revealed an increased CV risk in 21.2% and subclinical carotid atherosclerosis (SCA) in 85.7% of the patients, respectively. cfPWV and cIMT were higher in patients compared with controls (Padj=0.021 and Padj=0.003, respectively). In the ASyS group, cfPWV and cIMT correlated significantly with age (r = 0.679; P&lt;0.001 and r = 0.664; P&lt;0.001, respectively). Moreover, cfPWV correlated with BMI (Padj=0.001) and diabetes (Padj=0.043). CCA-RI and CCA-PI showed significant associations with creatine phosphokinase (r = 0.629; P=0.012 and r = 0.574; P=0.032, respectively) and ICA-RI and ICA-PI were higher in patients with lung involvement (both; P=0.039).

Conclusion
ASyS patients had higher aortic stiffness and SCA compared with controls, even after adjustment for confounders. SCORE/mSCORE performed poorly in identifying high-risk patients compared with cfPWV and carotid sonography. Thus, cfPWV and carotid sonography may improve CV and cerebrovascular screening in ASyS.
Identification of Flucloxacillin-Haptenated HLA-B*57:01 Ligands: Evidence of Antigen Processing and Presentation
Authors: Waddington James C , Meng Xiaoli , Illing Patricia T , Tailor Arun , Adair Kareena , Whitaker Paul , Hamlett Jane , Jenkins Rosalind E , Farrell John , Berry Neil , Purcell Anthony W , Naisbitt Dean J , Park Brian Kevin 
Published: July 2020

AbstractFlucloxacillin is a β-lactam antibiotic associated with a high incidence of drug-induced liver reactions. Although expression of human leukocyte antigen (HLA)-B*57:01 increases susceptibility, little is known of the pathological mechanisms involved in the induction of the clinical phenotype. Irreversible protein modification is suspected to drive the reaction through the modification of peptides that are presented by the risk allele. In this study, the binding of flucloxacillin to immune cells was characterized and the nature of the peptides presented by HLA-B*57:01 was analyzed using mass spectrometric-based immunopeptidomics methods. Flucloxacillin modification of multiple proteins was observed, providing a potential source of neoantigens for HLA presentation. Of the peptides eluted from flucloxacillin-treated C1R-B*57:01 cells, 6 putative peptides were annotated as flucloxacillin-modified HLA-B*57:01 peptide ligands (data are available via ProteomeXchange with identifier PXD020137). To conclude, we have characterized naturally processed drug-haptenated HLA ligands presented on the surface of antigen presenting cells that may drive drug-specific CD8+ T-cell responses.
MAVRIC: A Multicenter Randomized Controlled Trial of Transabdominal vs Transvaginal Cervical Cerclage
Authors: Shennan Andrew , Chandiramani Manju , Bennett Phillip , David Anna L. , Girling Joanna , Ridout Alexandra , Seed Paul T. , Simpson Nigel , Thornton Steven , Tydeman Graham , Quenby Siobhan , Carter Jenny 
Published: July 2020

(Abstracted from Am J Obstet Gynecol 2020;222:261.e1–261.e9)
Women who experience multiple late miscarriages or spontaneous preterm births may be candidates for vaginal cerclage. In women with a history of pregnancy loss with vaginal cerclage, observational studies have suggested that transabdominal cerclage (TAC) may be beneficial over high vaginal cerclage (HVC) and low vaginal cerclage (LVC).
Noninvasive Prenatal Detection of Hemoglobin Bart Hydrops Fetalis Via Maternal Plasma Dispensed With Parental Haplotyping Using the Semiconductor Sequencing Platform
Authors: Yang Jiexia , Peng Chun-fang , Qi Yiming , Rao Xing-qiang , Guo Fangfang , Hou Yaping , He Wei , Wu Jing , Chen Yang-yi , Zhao Xin , Wang Yu-nan , Peng Haishan , Wang Dongmei , Du Li , Luo Ming-yong , Huang Quan-fei , Liu Hai-liang , Yin Aihua 
Published: July 2020

(Abstracted from Am J Obstet Gynecol 2020;222:185.e1–185.e17)
Hemoglobin Bart's hydrops fetalis syndrome (Hb Bart's) is a common inherited genetic disease in the south of China and Southeast Asia. Couples who are both carriers of the Southeast Asian type of α-thalassemia gene deletion have a 25% risk of having a pregnancy affected with Hb Bart's as a result of autosomal recessive inheritance.
Clinical Factors Affecting the Dose Conversion Ratio from Intravenous to Oral Tacrolimus Formulation among Pediatric Hematopoietic Stem Cell Transplantation Recipients
Authors: Kanamitsu Kiichiro , Yorifuji Takashi , Ishida Hisashi , Fujiwara Kaori , Washio Kana , Shimada Akira , Tsukahara Hirokazu 
Published: July 2020

Background:
Tacrolimus is converted from intravenous to oral formulation for the prophylaxis of graft-versus-host disease when patients can tolerate oral intake and graft-versus-host disease is under control. Oral tacrolimus formulation presents poor bioavailability with intraindividual and interindividual variations; however, some factors affecting its blood concentration among pediatric hematopoietic stem cell transplantation (HCT) recipients are still unclear. This study aimed to identify the clinical factors affecting tacrolimus blood concentrations after switching its formulation.

Methods:
Changes in the blood concentration/dose ratio (C/D) of tacrolimus in pediatric HCT recipients were analyzed after the switching of tacrolimus from intravenous to oral formulation. Clinical records of 57 pediatric patients who underwent allogenic HCT from January 2006 to April 2019 in our institute were retrospectively reviewed. The C/D of tacrolimus before discontinuation of intravenous infusion (C/Div) was compared with the tacrolimus trough level within 10 days after the initiation of oral administration (C/Dpo). Multiple linear regression analysis was performed to identify factors affecting (C/Dpo)/(C/Div).

Results:
The constant coefficient of (C/Dpo)/(C/Div) was 0.1692 [95% confidence interval (CI), 0.137–0.2011]. The concomitant use of voriconazole or itraconazole and female sex were significant variables with a beta coefficient of 0.0974 (95% CI, 0.062–0.133) and −0.0373 (95% CI, −0.072 to −0.002), respectively.

Conclusions:
After switching of tacrolimus formulation, pediatric HCT recipients might need oral tacrolimus dose that is 5–6 and 3.5–4.5 times the intravenous dose to maintain tacrolimus blood concentrations and area under the concentration–time curve, respectively. With the concomitant use of voriconazole or itraconazole, an oral tacrolimus dose of 4–5 times the intravenous dose seemed appropriate to maintain blood tacrolimus concentration.
Hysteroscopic Metroplasty for T-Shaped Uterus: A Systematic Review and Meta-analysis of Reproductive Outcomes
Authors: Garzon Simone , Laganà Antonio Simone , Di Spiezio Sardo Attilio , Alonso Pacheco Luis , Haimovich Sergio , Carugno Jose , Vitale Salvatore Giovanni , Casarin Jvan , Raffaelli Ricciarda , Andrisani Alessandra , Zizolfi Brunella , Cromi Antonella , Ghezzi Fabio , Franchi Massimo , Vitagliano Amerigo 
Published: July 2020

Importance
To date, a comprehensive review on the safety and effectiveness of hysteroscopic metroplasty for T-shaped uterus is still missing.

Objective
To provide a robust synthesis of the available studies investigating reproductive outcomes after hysteroscopic metroplasty for T-shaped uterus.

Evidence Acquisition
We performed a systematic review and meta-analysis (CRD42019143291), using the proportion method with 95% confidence interval (CI). Statistical heterogeneity was assessed by Higgins test (I
2).

Results
We included 11 cohort studies embedding 937 women who underwent hysteroscopic metroplasty. After surgery, the pooled percentage of live birth was 44.54% (95% CI, 36.12%–53.12%; I
2 = 46.22%) and 56.88% (95% CI, 46.48%–66.98%; I
2 = 36.38%) in women with primary infertility and recurrent miscarriage, respectively. In women with recurrent miscarriage, the pooled proportion of miscarriage was 21.46% (95% CI, 15.09%–28.61%; I
2 = 30.18%). The pooled clinical pregnancy proportion in women with primary infertility was 57.19% (95% CI, 43.83%–70.03%; I
2 = 77.81%). The pooled rate of surgical complications was 0.65% (95% CI, 0.20%–1.33%; I
2 = 11.44%).

Conclusions
The hysteroscopic correction of T-shaped uteri was associated with high live birth rate and low miscarriage rate, both in case of primary infertility and recurrent miscarriage.

Relevance
Hysteroscopic metroplasty can be considered a safe and effective strategy to improve reproductive outcomes in case of T-shaped uterus.

Target Audience
Obstetricians and gynecologists, family physicians.

Learning Objectives
After participating in this activity, the learner should be better able to identify and diagnose T-shaped uterus; explain the epidemiological data regarding reproductive outcomes in cases of untreated T-shaped uterus; and describe the technique and outcomes of hysteroscopic metroplasty for T-shaped uterus, both in case of primary infertility and recurrent miscarriage.
Accurate Diagnosis of Endometriosis Using Serum MicroRNAs
Authors: Moustafa Sarah , Burn Martina , Mamillapalli Ramanaiah , Nematian Sepide , Flores Valerie , Taylor Hugh S. 
Published: July 2020

(Abstracted from Am J Obstet Gynecol 2020; doi: 10.1016/j.ajog.2020.02.050)
Endometriosis, a chronic inflammatory disorder causing pain and infertility, affects nearly 10% of reproductive age women. Despite its high prevalence, the average time from onset of symptoms to diagnosis is 5 to 10 years.
Chromosome Errors in Human Eggs Shape Natural Fertility Over Reproductive Life Span
Authors: Gruhn Jennifer R. , Zielinska Agata P. , Shukla Vallari , Blanshard Robert , Capalbo Antonio , Cimadomo Danilo , Nikiforov Dmitry , Chan Andrew Chi-Ho , Newnham Louise J. , Vogel Ivan , Scarica Catello , Krapchev Marta , Taylor Deborah , Kristensen Stine Gry , Cheng Junping , Ernst Erik , Bjørn Anne-Mette Bay , Colmorn Lotte Berdiin , Blayney Martyn , Elder Kay , Liss Joanna , Hartshorne Geraldine , Grøndahl Marie Louise , Rienzi Laura , Ubaldi Filippo , McCoy Rajiv , Lukaszuk Krzysztof , Andersen Claus Yding , Schuh Melina , Hoffmann Eva R. 
Published: July 2020

(Abstracted from Science 2019;365:1466–1469)
While it is understood that young females (≥13 years to early 20s) and women of advancing maternal age (AMA, mid-30s or older) have reduced fertility rates, the exact mechanisms behind this feature of the fertility curve remain unclear. Because chromosomal errors and aneuploidy cause a significant amount of pregnancy loss and congenital disorders in women of advanced maternal age, meiotic error rates throughout the entire reproductive life span may shape natural fertility.
Specific Birth Defects in Pregnancies of Women With Diabetes: National Birth Defects Prevention Study, 1997–2011
Authors: Tinker Sarah C. , Gilboa Suzanne M. , Moore Cynthia A. , Waller D. Kim , Simeone Regina M. , Kim Shin Y. , Jamieson Denise J. , Botto Lorenzo D. , Reefhuis Jennita 
Published: July 2020

(Abstracted from Am J Obstet Gynecol 2020;222:176.e1–176.e11)
The National Birth Defects Prevention Study (NBDPS) was one of the first analyses to report the association between diabetes and birth defects. The study’s data of approximately 18,000 deliveries from October 1997 to December 2003 showed strong associations with many birth defects, primarily with pregestational diabetes but also with gestational diabetes.
Factors Affecting Implementation of Evidence-Based Practices in Public Health Preparedness and Response
Authors: Kennedy Mallory , Carbone Eric G. , Siegfried Alexa L. , Backman Deborah , Henson John D. , Sheridan Jackie , Meit Michael B. , Thomas Erin V. 
Published: July 2020

Context:
There is limited research on what factors are most salient to implementation of evidence-based practices (EBPs) among public health agencies in public health emergency preparedness and response (PHPR) and under what conditions EBP implementation will occur.

Objective:
This study assessed the conditions, barriers, and enablers affecting EBP implementation among the PHPR practice community and identified opportunities to support EBP implementation.

Design:
A Web-based survey gathered information from public health agencies. Data obtained from 228 participating agencies were analyzed.

Setting:
State, local, and territorial public health agencies across the United States.

Participants:
Preparedness program officials from 228 public health agencies in the United States, including Public Health Emergency Preparedness (PHEP) cooperative agreement awardees (PHEP awardees) and a random sample of local health departments (LHDs).

Results:
Respondents indicated that EBP is necessary and improves PHPR functions and tasks and that staff are interested in improving skills for EBP implementation. Top system-level barriers to EBP implementation were insufficient funding, lack of EBP, and lack of clarity regarding which practices are evidence based. PHEP awardees were significantly more likely to report a lack of EBP in the field, whereas LHDs were significantly more likely to report a lack of incentives. The top organizational-level barrier was insufficient staff. Most respondents indicated their agency culture supports EBP; however, LHDs were significantly more likely to report a lack of support from supervisors and leadership. Few respondents reported individual barriers to EBP implementation.

Conclusions:
Findings indicate an opportunity to improve dissemination strategies, communication efforts, and incentives to support EBP implementation in PHPR. Potential strategies include improving awareness of and accessibility to EBPs through targeted dissemination efforts; building organizational capacity to support EBP implementation, particularly staff capacity, knowledge, and skills; and identifying funding and incentives to promote EBP uptake and sustainment.
Association Between Accreditation Engagement and Using The Community Guide for Preventive Services by Local Health Departments
Authors: Roba Assegid Aga , Williams Brianna C. , Waterfield Kristie C. , Shah Gulzar H. 
Published: July 2020

Background:
Accreditation is one of the macro trends shaping the future of public health practice, and The Community Guide to Preventive Services is a decision-making tool used to select evidence-based interventions. Although local health departments (LHDs) seeking accreditation use The Community Guide, the relationship between the two remains unknown. The purpose of this study was to determine whether accreditation engagement is associated with the extent to which LHDs use The Community Guide.

Methods:
Data from the National Association of County and City Health Officials' 2016 Profile of LHDs were examined for associations between accreditation engagement and the use of The Community Guide.

Results:
Whereas 52.8% of LHDs used The Community Guide sometimes or consistently, only 21.5% were engaged in the accreditation process. Engaged LHDs were more likely to use The Community Guide consistently (adjusted odds ratios: 8.2; 95% confidence interval, 4.0-16.4) or sometimes (adjusted odds ratios: 3.6; 95% confidence interval, 2.4-5.3) than those not engaged at all. Local health departments serving a population of more than 50 000, organized with a county or mixed level of jurisdiction, and those having a top executive with a masters' or a doctoral degree, were more likely to report some use of The Community Guide.

Conclusions:
The extent that LHDs utilize The Community Guide is positively associated with the level of their accreditation engagement. Capacity building and policy measures could encourage LHDs to seek accreditation thereby increasing the use of The Community Guide for evidence-informed practice. Future studies should identify causal factors predicting the use of The Community Guide.
A Quality Initiative to Improve Appropriate Use of Initial Outpatient Echocardiography Among Pediatric Cardiologists
Authors: Frandsen Erik L. , Kourtidou Soultana , Tieder Joel S. , Alberda Erin , Soriano Brian D. 
Published: July 2020

Introduction:
Appropriate use criteria (AUC) guide initial transthoracic echocardiogram (TTE) use in outpatient pediatrics. We sought to improve pediatric cardiologist TTE ordering appropriateness (mean AUC score) with a quality improvement initiative.

Methods:
The outcome of interest was the prospective AUC score for all initial outpatient TTEs ordered between November 2016 and August 2017, categorized per the AUC: “appropriate” (score 7–9), “may be appropriate” (4–6), “rarely appropriate” (1–3). Interventions included a didactic review of 2014 AUC and participant documentation of AUC criteria for each TTE. Participants met quarterly to evaluate outcome, process, and balancing measures, intervention effectiveness, and to identify and mitigate barriers.

Results:
Twenty-two pediatric cardiologists participated. TTE appropriateness level before (n = 216) and after (n = 557) intervention was high. There was no significant difference in mean baseline and post-intervention AUC score (7.42 ± 1.87 versus 7.16 ± 2.87, P = 0.1), nor in TTE sensitivity (27% versus 25%, P > 0.1) as a balancing measure. Among baseline studies, 81% were “appropriate,” and 6% “rarely appropriate.” Among post-intervention studies, 76% were “appropriate,” and 11% “rarely appropriate.” Barriers identified to implementing AUC include TTE indications not specified by current AUC, expectations of referring provider or parent to perform TTE, consistent provider application of AUC, and ability of AUC to capture comprehensive clinical judgment.

Conclusions:
Although the mean AUC appropriateness level was high, we were able to identify significant barriers to the implementation of AUC. Future efforts should focus on the reduction of “rarely appropriate” TTE ordering.
Draconibacterium mangrovi sp. nov., isolated from mangrove sediment
Authors: Hu Yuzhong , Guo Yu , Lai Qiliang , Dong Le , Huang Zhaobin 
Published: July 2020

A marine bacterial strain, designated GM2-18T, was isolated from mangrove sediment sampled at Luoyang River estuary, Quanzhou, PR China. Cells were Gram-stain-negative, slightly curved long rod-shaped and facultatively anaerobic with no flagellum. Catalase activity was found to be weak-positive and oxidase-positive. It had no ability to degrade or hydrolyse substrates including skimmed milk, cellulose, starch and Tweens (40, 60 and 80). The 16S rRNA gene sequence of strain GM2-18T had maximum similarity values to ‘

Draconibacterium filum

’ F2T,

Draconibacterium sediminis

JN14CK-3T and

Draconibacterium orientale

FH5T of 98.0, 97.8 and 97.4 %, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GM2-18T was affiliated to the genus

Draconibacterium

and formed a clade with an uncultured bacterium clone identified from mangrove environment. Average nucleotide identity values and DNA–DNA hybridization estimates of strain GM2-18T compared to its

Draconibacterium

relatives strongly supported that it belonged to a new species. The respiratory quinone was menaquinone MK-7. The major fatty acids (>10 %) consisted of iso-C15 : 0, anteiso-C15 : 0 and C17 : 1
ω6c. The polar lipids were phosphatidylethanolamine, a phospholipid and several unidentified lipids. The genomic size of strain GM2-18T was 5.9 Mb and the G+C content was 40.8 mol%. Gene prediction and annotation of strain GM2-18T indicated that there was a nitrogen-fixing gene cluster encoding nitrogenase molybdenum-iron protein and related proteins responsible for nitrogen fixation. Based on the above characteristics, strain GM2-18T represents a novel species within the genus

Draconibacterium

. Thus, Draconibacterium mangrovi sp. nov. is proposed with type strain GM2-18T (=MCCC 1K04382T=KCTC 72879T), isolated from mangrove sediment.