bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐term Outcomes of Computerized Tomography–Guided Sphenopalatine Ganglion–Targeted Pulsed Radiofrequency for Refractory Cluster Headache
Authors: Chen Zheng , Ren Hao , Zhao Chunmei , Luo Fang 
Published: November 2019

AbstractObjectiveTo further evaluate the efficacy and safety of computerized tomography–guided sphenopalatine ganglion–targeted pulsed radiofrequency treatment for patients with refractory episodic and chronic cluster headache (CH).MethodsForty‐five patients with refractory episodic CH and 14 patients with chronic CH who underwent computerized tomography–guided sphenopalatine ganglion–targeted pulsed radiofrequency between January 2011 and December 2018 at the Beijing Tiantan Hospital were included and analyzed in this retrospective cohort study.ResultsA total of 59 patients underwent 106 computerized tomography–guided pulsed radiofrequency procedures throughout the observational period. Effective remission was observed in 95.6% and 64.3% of patients with refractory episodic and chronic CH, respectively. Repeated computerized tomography–guided sphenopalatine ganglion–targeted pulsed radiofrequency procedures for recurrent CH was also proven to be effective. No severe side effects or complications were observed in this study.ConclusionThe computerized tomography–guided sphenopalatine ganglion–targeted pulsed radiofrequency procedure is an effective, safe, and repeatedly effective strategy for refractory CH. For patients who have not responded to conservative treatment, this minimally invasive intervention is a reliable alternative.
A multidrug and toxic compound extrusion (MATE) transporter modulates auxin levels in root to regulate root development and promotes aluminium tolerance
Authors: Upadhyay Neha , Kar Debojyoti , Datta Sourav 
Published: November 2019

AbstractMATE (multidrug and toxic compound extrusion) transporters play multiple roles in plants including detoxification, secondary metabolite transport, aluminium (Al) tolerance, and disease resistance. Here we identify and characterize the role of the Arabidopsis MATE transporter DETOXIFICATION30. AtDTX30 regulates auxin homeostasis in Arabidopsis roots to modulate root development and Al‐tolerance. DTX30 is primarily expressed in roots and localizes to the plasma membrane of root epidermal cells including root hairs. dtx30 mutants exhibit reduced elongation of the primary root, root hairs, and lateral roots. The mutant seedlings accumulate more auxin in their root tips indicating role of DTX30 in maintaining auxin homeostasis in the root. Al induces DTX30 expression and promotes its localization to the distal transition zone. dtx30 seedlings accumulate more Al in their roots but are hyposensitive to Al‐mediated rhizotoxicity perhaps due to saturation in root growth inhibition. Increase in expression of ethylene and auxin biosynthesis genes in presence of Al is absent in dtx30. The mutants exude less citrate under Al conditions, which might be due to misregulation of AtSTOP1 and the citrate transporter AtMATE. In conclusion, DTX30 modulates auxin levels in root to regulate root development and in the presence of Al indirectly modulates citrate exudation to promote Al tolerance.
Day length regulates seasonal patterns of stomatal conductance in Quercus species
Authors: Granda Elena , Baumgarten Frederik , Gessler Arthur , Gil‐Pelegrin Eustaquio , Peguero‐Pina Jose Javier , Sancho‐Knapik Domingo , Zimmermann Niklaus E. , Resco de Dios Víctor 
Published: November 2019

AbstractVapour pressure deficit is a major driver of seasonal changes in transpiration, but photoperiod also modulates leaf responses. Climate warming might enhance transpiration by increasing atmospheric water demand and the length of the growing season, but photoperiod‐sensitive species could show dampened responses. Here, we document that day length is a significant driver of the seasonal variation in stomatal conductance. We performed weekly gas exchange measurements across a common garden experiment with 12 oak species from contrasting geographical origins, and we observed that the influence of day length was of similar strength to that of vapour pressure deficit in driving the seasonal pattern. We then examined the generality of our findings by incorporating day‐length regulation into well‐known stomatal models. For both angiosperm and gymnosperm species, the models improved significantly when adding day‐length dependences. Photoperiod control over stomatal conductance could play a large yet underexplored role on the plant and ecosystem water balances.
Relationships of growth, stable carbon isotope composition and anatomical properties of leaf and xylem in seven mulberry cultivars: a hint towards drought tolerance
Authors: Cao X. , Shen Q. , Liu L. , Cheng J. 
Published: November 2019

Abstract

The fast growth of mulberry depends on high water consumption, but considerable variations in drought tolerance exist across different cultivars. Physiological and anatomical mechanisms are important to plant survival under drought. However, few research efforts have been made to reveal the relationships of these two aspects in relation to drought tolerance.

In this study, growth rates, leaf functional physiology and anatomical characteristics of leaf and xylem of 1‐year‐old saplings of seven mulberry cultivars at a common garden were compared. Their relationships were also explored.

Growth, leaf physiology and anatomy were significantly different among the tested cultivars. Foliar stable carbon isotope composition (δ13C) was negatively correlated with growth rates, and closely related to several leaf and xylem anatomical traits. Particularly, leaf thickness, predicted hydraulic conductivity and vessel element length jointly contributed 77% of the variability in δ13C. Cultivar Wupu had small stomata, intermediate leaf thickness, the smallest hydraulically weighted vessel diameter and highest vessel number, and higher δ13C; Yunguo1 had high abaxial stomatal density, low specific leaf area, moderate hydraulic conductivity and δ13C; these are beneficial features to reduce leaf water loss and drought‐induced xylem embolism in arid areas. Cultivar Liaolu11 had contrasting physiological and anatomical traits compared with the previous two cultivars, suggesting that it might be sensitive to drought.

Our findings indicate that growth and δ13C are closely associated with both leaf and xylem anatomical characteristics in mulberry, which provides fundamental information to assist evaluation of drought tolerance in mulberry cultivars and in other woody trees.
Genetic analysis suggests a long and largely isolated evolutionary history of plum pox virus strain D in Turkey
Authors: Gürcan K. , Teber S. , Candresse T. 
Published: November 2019

Plum pox virus (PPV) strain D is globally distributed and causes serious losses in stone fruits in over 40 countries. Here, full‐length genomic sequences were analysed for 44 PPV‐D isolates from all regions of Turkey, together with partial sequences for a larger number of isolates. PPV‐D isolates from Turkey are similar to other PPV‐D isolates in all major genomic features. However, the majority of Turkish PPV‐D isolates form separate phylogenetic clusters from all other isolates and show a geographical clustering tendency, suggestive of limited movement between regions. In particular, PPV‐D isolates from Thrace and Central Anatolia formed a monophyletic sister cluster to the cluster that includes all previously known PPV‐D isolates. Two isolates with strong evidence of recombination with the PPV‐T strain were identified, together with two isolates with weaker evidence for intra‐D strain recombination. The genetic diversity of PPV‐D was found to be particularly high in Turkey (0.017 ± 0.001%), close to that observed for PPV‐D world diversity once the over‐represented isolates from Japan, the USA and Canada have been excluded (0.020 ± 0.001%). Taken together, these results suggest a long and largely isolated evolutionary history of PPV‐D in Turkey and further extend knowledge of the diversity of this highly successful strain. The high diversity of PPV‐D in Turkey, together with the basal phylogenetic position of Turkish isolates, are compatible with a hypothesis making Turkey the centre of origin of the D strain.
Formation, diagenesis and palaeoenvironmental significance of upper Ediacaran fibrous dolomite cements
Authors: Hu Yongjie , Cai Chunfang , Liu Dawei , Pederson Chelsea L. , Jiang Lei , Shen Anjiang , Immenhauser Adrian 
Published: November 2019

AbstractNeoproterozoic marine dolomite cements represent reliable, albeit complex, archives of their palaeoenvironment. Petrological and high‐resolution geochemical data from well‐preserved fibrous dolomite and pyrite in the upper Ediacaran (ca 551·1 to 548·0 Ma) Dengying Formation in south‐west China are presented and discussed here. The aim of this research is to reconstruct the redox state of late Ediacaran shallow seawater and porewater in the Sichuan Basin using early marine diagenetic fabrics. Based on crystalline texture and axis, four basic types of fibrous dolomite cements formed penecontemporaneously in a microbialite reef setting at the platform margin: (i) bladed dolomites (replacement from a high‐Mg calcite precursor); (ii) fascicular fast dolomites (replacement from an aragonitic precursor); (iii) fascicular slow dolomites; and (iv) radial slow dolomites. The latter two fabrics are considered direct marine porewater precipitates due to their length‐slow character, cathodoluminescent zonation, and enriched copper and cobalt concentrations. Marine cements yield rare earth element and yttrium patterns comparable to modern seawater and represent a refined set of archive data relative to previously published bulk dolostones. Redox‐sensitive elements and cathodoluminescence indicate that the fascicular fast dolomites formed in suboxic seawater, while fascicular slow and radial slow dolomites formed in euxinic marine porewaters. Microbial sulphate reduction during the formation of fascicular slow and radial slow dolomites is recognized by nanometre‐scale spheroidal ankerite and sulphur‐containing dolomite, and intergrown pyrite grains with U‐shaped δ34S transects. Data shown here suggest predominantly suboxic shallow late Ediacaran seawater and euxinic marine porewaters, with microbial activity promoting the direct precipitation of dolomite.
Incidence, features, and outcomes of cytomegalovirus DNAemia in unmanipulated haploidentical allogeneic hematopoietic stem cell transplantation with post‐transplantation cyclophosphamide
Authors: Huntley Dixie , Giménez Estela , Pascual María Jesús , Hernández‐Boluda Juan Carlos , Gago Beatriz , Vázquez Lourdes , Piñana José Luis , García Magdalena , Pérez Ariadna , Serrano David , Hernández Marta , Albert Eliseo , Solano Carlos , Navarro David 
Published: November 2019

AbstractBackgroundConflicting data have been published as to the risk of cytomegalovirus (CMV) DNAemia and CMV disease in patients undergoing haploidentical hematopoietic stem cell transplantation (haplo‐HSCT) with post‐transplantation cyclophosphamide.MethodsWe conducted a multicenter retrospective study including 118 patients subjected to unmanipulated haplo‐HSCT to further clarify this issue. An historic cohort comprising 165 patients undergoing other transplant modalities (HLA‐matched related, matched unrelated or mismatched) was built for comparison purposes. Plasma CMV DNA monitoring was performed using two highly sensitive real‐time PCR assays.ResultsOverall, the cumulative incidence of CMV DNAemia, recurrent CMV DNAemia, and CMV DNAemia requiring preemptive antiviral therapy in patients undergoing haplo‐HSCT was 63.9%, 34.9%, and 50.1%, respectively. These figures were rather comparable for other transplant modalities (P = .22,P = .13 andP = .72, respectively). A trend toward longer duration of episodes and shorter CMV DNA doubling times was observed in haplo‐HSCT patients in comparison with other transplant modalities. Furthermore, median CMV DNA peak load was significantly higher in haplo‐HSCTs (P = .008), yet overall mortality by day 180 and 365 was the same across comparison groups. There were five cases of CMV disease, and all occurred in haplo‐HSCT patients. This latter observation is worrying and merits further investigation.ConclusionsThe incidence of initial and recurrent episodes of CMV DNAemia either requiring or not antiviral therapy in unmanipulated haplo‐HSCT was comparable to other transplant modalities in our cohort.
Quantitative ubiquitylomics approach for characterizing the dynamic change and extensive modulation of ubiquitylation in rice seed germination
Authors: He Dongli , Li Ming , Damaris Rebecca N. , Bu Chen , Xue Jianyou , Yang Pingfang 
Published: November 2019

SummaryDuring seed germination, cells embark on extensive post‐transcriptional and post‐translational modifications (PTM), providing a perfect platform to study these events in embryo rebooting from relative quiescenct to highly active state. PR‐619, a deubiquitylase inhibitor, delayed the rice seed germination and resulted in the accumulation of ubiquitylated proteins, which indicated the protein ubiquitylation is involved in this process. Using the K‐Ɛ‐GG antibody enrichment method integrated with high‐resolution mass spectrometry, a list of 2576 lysine ubiquitylated (Kub) sites in 1171 proteins was compiled for rice embryos at 0, 12 and 24 h after imbibition (HAI). Of these, the abundance of 1419 Kub sites in 777 proteins changed significantly. Most of them substantially increased within the first 12 HAI, which is similar to the dynamic state previously observed for protein phosphorylation, implying that the first 12 HAI are essential for subsequent switch during rice seed germination. We also quantitatively analyzed the embryo proteome in these samples. Generally, a specific protein’s abundance in the ubiquitylome was uncorrelated to that in the proteome. The differentially ubiquitinated proteins were greatly enriched in the categories of protein processing, DNA and RNA processing/regulation related, signaling, and transport. The DiGly footprint of the Kub sites was significantly reduced on K48, a linkage typically associated with proteasome‐mediated degradation. These observations suggest ubiquitylation may modulate the protein function more than providing 26S degradation signals in the early stage of rice seed germination. Revealing this comprehensive ubiquitylome greatly increases our understanding of this critical PTM during seed germination.
Valuing water supply infrastructure improvements using life satisfaction data as a complement to contingent valuation
Authors: del Saz‐Salazar Salvador , González‐Gómez Francisco , Guardiola Jorge 
Published: November 2019

AbstractIn light of the growing interest and potential of the Life Satisfaction Approach (LSA) to value environmental attributes, this research paper aims to estimate the monetary value of water supply infrastructure improvements using this novel approach. A theoretical framework compares this method with the widely used contingent valuation method (CVM), highlighting the pros and cons of both approaches. Results show that the LSA can generate meaningful values of the nonmarket benefits derived from improving water supply infrastructure, based on the impact that such benefits can have on individuals’ life satisfaction. In addition, these values are not different from those obtained from the CVM. Thus the LSA arises as a useful complement to the more traditional methods while expanding the array of techniques available for the economic valuation of environmental goods in a cost benefit framework.
Physiological processes of inflammation and edema initiated by sustained mechanical loading in subcutaneous tissues: A scoping review
Authors: Van Damme Nele , Van Hecke Ann , Remue Eline , Van den Bussche Karen , Moore Zena , Gefen Amit , Verhaeghe Sofie , Beeckman Dimitri 
Published: November 2019

ABSTRACTDeep tissue injuries are pressure ulcers which initiate in the subcutaneous tissues and extend through a bottom‐up pathway. Once deep tissue injuries are visual at skin level, serious irreversible tissue damage has already occurred. In pressure ulcer development, inflammation and edema are coupled physiological processes associated with tissue damage arising due to sustained mechanical loading. This study aimed to provide an in‐depth overview of the physiological processes of inflammation and edema initiated by sustained mechanical loading in subcutaneous tissues, in the context of pressure ulceration. A scoping review was performed according to the framework by Arksey and O'Malley. The databases MEDLINE, EMBASE, Web of Science, and Scopus, and the reference lists of included studies were searched for in vivo (animal, human), and in vitro studies matching the study objectives (from inception to 28 May 2018). No restrictions for inclusion were applied for study design, setting, participants, and year of publication. A total of 12 studies were included, varying in study design, sample characteristics, amount and duration of mechanical loads that were applied, follow‐up time, and assessment methods. Neutrophil infiltration and edema occur in the subcutaneous tissues nearly immediately after the application of load on soft tissues. The amount of neutrophils and edema increase in the first days after the mechanical insult and decrease once healing has been initiated and no supplementary mechanical load was applied. One study indicated that edema may extend up to the level of the dermo‐epidermal junction. Further research should focus on how deep tissue inflammation and edema are reflected into unique tissue changes at skin level, and how abnormal inflammatory responses manifest (e.g. when the nervous system is not functioning normally).
Coxiella burnetiiseroprevalence in unvaccinated veterinary workers in Australia: Evidence to support Q fever vaccination
Authors: Sellens Emily , Bosward Katrina L. , Norris Jacqueline M. , Wood Nicholas , Heller Jane , Graves Stephen , Gidding Heather F. 
Published: November 2019

AbstractQ fever (caused byCoxiella burnetii) is a serious zoonotic disease that occurs almost worldwide. Occupational contact with animals increases the risk of exposure, and Q fever vaccination is recommended for veterinary workers in Australia. This study aimed to investigateC. burnetiiseroprevalence among unvaccinated veterinary workers in Australia and determine factors associated with a positive serological result. During 2014 and 2015, convenience sampling at veterinary conferences and workplace vaccination clinics was undertaken. Participants completed a questionnaire and provided a blood sample forC. burnetiiserology. Participants were predominantly veterinarians (77%), but veterinary support staff, animal scientists, and administration workers also participated. Blood samples (n = 192) were analysed by an immunofluorescence assay and considered positive where the phase I or phase II IgG titre was ≥1/50. Seroprevalence was 19% (36/192; 95% CI 14%–25%). A positive serological result was significantly associated with (a) working in outer regional/remote areas (odds ratio [OR] 6.2; 95% CI 1.9–20.8; reference = major cities;p = .009) and (b) having spent more than 50% of total career working with ruminants (OR 4.8; 95% CI 1.7–13.5; reference = <15% of career;p = .025). These findings confirm an increased risk of exposure toC. burnetiicompared to the general population, providing new evidence to support Q fever vaccination of veterinary workers in Australia.
On the Convergence of the Matrix Lambert W Approach to Solution of Systems of Delay Differential Equations
Authors: Galip Ulsoy A. , Gitik Rita 
Published: November 2019

Abstract
Convergence of the matrix Lambert W function method for solving systems of delay differential equations (DDEs) is considered. Recent research shows that convergence problems occur with certain DDEs when using the well-established Q-iteration approach. A complementary, and recently proposed, W-iteration approach is shown to converge even on systems where Q-iteration fails. Furthermore, the role played by the branch numbers k = −∞ … −1, 0, 1, … ∞ of the matrix Lambert W function, Wk, in terms of initializing the iterative solutions, is also discussed and elucidated. Several second-order examples, known to have convergence problems with Q-iteration, are readily solved by W-iteration. Examples of third- and fourth-order DDEs show that W-iteration is also effective on higher-order systems.
Early-onset sepsis: can we screen fewer babies safely?
Authors: Eason Julian , Ward Hope , Danko Olha , Richardson Kerrie , Vaitkute Rima , McKeon-Carter Roisin 
Published: November 2019

BackgroundOver the past 2–3 years at the Southwest Peninsula tertiary neonatal unit in Plymouth, the authors have observed an increase in the number of clinically well term infants being screened and treated with antibiotics for infection in accordance with NICE guidance. The aim of our study was to assess the safety of implementing the Kaiser Permanente Early Onset Sepsis (KPEOS) calculator to minimise antibiotic usage in term infants in line with antimicrobial stewardship, reducing separation from mother at birth and facilitating earlier discharge.MethodsA 2-year retrospective review of medical records from 2014 to 2015 inclusive revealed 9217 deliveries, with 1550 infants (16.8%) having risk factors, 945 (10.2%) being term infants. Of those, 507 (53.6%) had a clinical reason to screen and 438 (46.4%) had risk factors alone treated with antibiotics for variable periods of time. This enabled us to review our usual practice and compare it with our KPEOS implementation.InterventionNational Health Service England permission was obtained to implement the KPEOS for a 6-month period. We collected data on all 175 term infants with risk factors to compare with our previous practice when The National Institute for Health and Care Excellence and Royal College of Obstetrics and Gynaecology maternal guidance was being followed.ResultsThe percentage of infants screened with a suspected infection previously receiving 5 days of antibiotics reduced from 31% (136/438) to 5% (9/157, p<0.0001) using the KPEOS calculator. Clinically well infants with risk factors alone previously receiving 36 hours of antibiotics, reduced from 63% (275/438) to 3% (5/157, p<0.0001) of infants treated. There was no late-onset sepsis in this study cohort or any observed adverse outcomes.ConclusionThese results demonstrated a potentially safe and effective quality improvement (QI) in our hospital with fewer babies treated and a reduced length of stay for this cohort. Considering individual hospitals rates for term Group B Streptococcal sepsis, this QI may be a safe and economical alternative to current practices for screening well term infants.