bluuebunny/crossref_metadata_2025_split huggingface dataset:
Augmented plate causes bone resorption in reconstruction of large segmental femur defects with induced membrane technique
Authors: Yu Shengpeng , Yin Bao , Wang Shulin , Jia Chao , Li Shu , Xie Zhao 
Published: August 2020

Abstract
Purposes: Large segmental femur bone defect is a serious clinical problem. Limited study have been reported to optimize the fixation during the second stage of induced membrane technique. We would like to report our new findings in two different fixation method with this technique. Methods: We retrospectively analyzed 56 cases with large segmental femur defects and treated with induced membrane technique from January 2013 to December 2017. Patients were separated into two groups according to different fixation method during the second stage and followed at least 2 years. Bacteria culture, treatment outcome, and complication were collected and analysed. Results: Patients with an average age of 41.8 years old had an average of 2.6 surgical history. Bone defects were 8.93 cm in average ( 4-16.8 cm). Though 20 complications were noticed in this series, 85.7% (48/56) of the cases acquired bone union during their final follow up. However, 57.6%(15/26) of the cases observed obvious bone resorption near the plate in augmented plate group. And this resulted to lower bone union rate was calculated when compared with intramedullary nail group (P = 0.019). Insufficient bone grafting material is another main reason resulted nonunion and verified in 5 cases. Conclusions: Augmented plate will resulted bone resorption and leads to nonunion in induced membrane technique. The plate should be fixated outside the membrane if necessary. Insufficient bone grafting material when grafting is a easily neglectful problem, and proper measures should be taken with cautious.
The effect of limited versus no tourniquet use on rapid recovery in total knee replacement--a randomized controlled trial
Authors: Yin Jiandong , Wang XinLing , Zuo Biao , Chen Fei , Yu Yang 
Published: August 2020

Abstract
Background: Tourniquets are widely used in TKA, but few articles state the difference in postoperative effects between restricting tourniquet use and not.Objective:We hoping that this experiment will look at the effect of limited tourniquet use on enhanced recovery in TKA. Methods: 150 patients who received TKA in the Affiliated Hospital of Chengdu University of TCM from June 2018 to December 2019 were randomly selected and randomly divided into 3 groups: ①limited use tourniquet group: after the completion of osteotomy, tourniquet was applied only when the prosthesis was installed, ②second half tourniquet group: Install prosthesis until suture is complete ③ no tourniquet group: no tourniquet was used throughout. The postoperative venous blood hemoglobin, C-reactive protein, visual analogue scale and HSS score were compared among the three groups. Results: Compared with the restricted use of tourniquet group, the visual analogue scale of knee joint pain and venous blood C-reactive protein level at 1 and 3 d after operation and hemoglobin level at 1 d after operation in the second half of tourniquet group increased and the HSS score at 3 and 7 d after operation decreased (P <0.05).No significant differences in visual analogue scale,HSS score,C-reactive,and hemoglobin between the restricted tourniquet and the no tourniquet. Conclusion: The results confirmed that the use of tourniquet in the second half of TKA would aggravate the early postoperative pain. In the effect of early rehabilitation, there is no significant between limited use of tourniquet or no use.
Assessment of Intracellular Calcium and Plasmalemmal Membrane Potential in Cryopreserved Metaphase II Mouse Oocytes
Authors: Ammar Omar Farhan , Moodley Therishnee 
Published: August 2020

Abstract
Objectives: Ca2+ is critical for normal oocyte activation and fertilization, and any alteration to the Ca2+ homeostasis may lead to failed fertilization or even cell death. It has been shown that intracellular Ca2+ is increased in bovine and human oocytes when cultured in vitro. Additionally, ATP sensitive potassium channels have been characterised recently in human and Xenopus oocytes Glibenclamide a KATP channel blocker was shown to protect human oocytes from Ca+2 overloading. None of these studies have been conducted in mouse oocytes to determine if they are a suitable alternative to human oocytes in the research setting. Thus, this research note aims to demonstrate if cryopreserved metaphase II (MII) mouse oocytes show similar Ca+2 and plasmalemmal membrane potential dynamics to those in human oocytes. Also, to show if glibenclamide influences Ca+2, and plasmalemmal membrane potential in cryopreserved metaphase II mouse oocytes. Results: our data did not show an increase in intracellular Ca2+ in untreated cryopreserved mouse oocytes loaded with Fluo-3 AM dye. However, an increase in the plasmalemmal membrane potential was noticed (hyperpolarization). Glibenclamide has shown no significant effect on Ca+2, mitochondrial and plasmalemmal membrane potential.
Experiences of Patients Undergoing Bowel Preparation and Colonoscopy- A Qualitative Longitudinal Study
Authors: Shamim Sara , Andresen Yvette Lena Margareta , Thaysen Henriette Vind , Jakobsen Ida Hovdenak , Nielsen Jannie , Danielsen Anne Kjærgaard , Konradsen Hanne 
Published: August 2020

Abstract
Background: Colonoscopy remains the reference standard for diagnosing and monitoring colorectal cancer and is also used for both diagnosis and surveillance of inflammatory bowel disease and it. Previous studies have shown that the preparation procedure was unpleasant for the patients, and they felt anxious about it alongside the anxiety related to the colonoscopy and the results. However, there is a limited knowledge of the patients’ needs when undergoing colonoscopy and the challenges within. The purpose of the study was to explore experiences of undergoing bowel preparation and colonoscopy.Methods: The study was designed as a qualitative longitudinal interview study with an inductive research approach. Twenty-five face to face interviews and twenty-one follow up interviews were conducted based on a semi structured interview guide. The interviews were analyzed using qualitative content analysis, and results reported according to COREQ guidelines.Results: Based on the findings, three categories emerged: To weigh up participation, A greater challenge than expected, and Not so challenging after all. Throughout these categories the experience of uncertainty was reported.Conclusions: The process of undergoing bowel preparation and colonoscopy was complex and influenced by uncertainty due to insufficient information. There is a need to strengthen the patient-centered care by adjusting the information to patient’s needs. It is necessary to tailor the written information to meet patients’ needs to better support them in decision-making for participation, to better prepare them for the bowel preparation and to better prepare them for the procedure. In addition, it is vital that patients are provided with results of the colonoscopy that correspond to the timeframe specified in written the information.
Pathways of Nurses in Long-term Care Hospitals: Seeking Work-life Balance
Authors: Kim Samsook , Lee Ga Eon , Bowers Barbara , Jo Yeonjae 
Published: August 2020

Abstract
Background: This study explores the pathways of nurses in LTCHs and its underlying conditions in Korea. Nursing shortage in long-term care setting has been a worldwide concern. Although the factors of staying or leaving of nurses in long term care hospitals (LTCHs) have been reported, few studies have examined the trajectory and conditions of nurses staying in and leaving LTCHs.Methods: A qualitative study design with a grounded theory approach was conducted. Data were collected in one to one interviews. Purposive and theoretical sampling led to the inclusion of 17 registered nurses with diverse characteristics from 14 LTCHs in a metropolitan city in South Korea. Individual, in-depth interviews were conducted. Data collection and analysis coincided. Data were analyzed using open and focused coding, constant comparison, theoretical sampling and memoing, and this enabled the construction of a substantive theory.Results: Seeking work-life balance was the core category of nurses’ pathway. Consequences of nurses’ pathway were categorized into three groups: thriving, surviving, and leaving. Thriving nurses in LTCHs found meaning in their work, fostered good relationships, and saw opportunities for growth. Surviving nurses in LTCHs were enduring their jobs in LTCHs, having a work-life balance, and supportive nursing leader. Leaving group nurses wished to leave LTCHs due to lack of professional growth, unappealing work, continuing conflict, and social stigma. However, compensation, work system, and interpersonal relationships were the common moving conditions for all three groups.Conclusions: This study provided the trajectory and conditions of nurses to enter, stay, move, or leave at LTCHs. Understanding the pathways for staying or leaving can be used as a strategy for successful retention of registered nurses in LTCHs.
The observation on curative effect of Shouhuitongbian capsule in the treatment of functional constipation: A real world study
Authors: Zhang Hongxi , Han Bao , Zhang Zhiyun , Yu Yongduo , Tian Zhenguo 
Published: August 2020

Abstract
Background: Chronic constipation is a common disease caused by a variety of reasons. This study was to observe the improvements on symptoms, quality of life and satisfaction of patients with functional constipation by Shouhuitongbian capsule, and evaluate its safety.Methods: The study was divided into four groups according to the duration of disease. The primary observational indicators were efficacy and safety of Shouhuitongbian capsule after 14 days of treatment, and changes of Wexner score of intestinal function. Effect of Shouhuitongbian capsule on quality of life of patients, and the long-term efficacy and safety of taking capsule after 1 month, 2 months and 3 months were used as secondary observational indicators .Results: The results showed that the improvement effect of capsules on 14th day was better that on the 7th day (P＜0.001). Besides, compared with patients with longer duration of disease, the improvement effect of patients with shorter duration of disease was better (P＜0.001). The long-term improvement effect of patients taking capsules was greater than the short-term improvement effect (P＜0.001). The long-term improvement of patients with shorter duration of disease after taking capsules was more effective than that of patients with longer duration of disease (P＜0.001).Conclusion: The experimental results provide a strong/powerful/ convincing evidence for the re-evaluation of efficacy and safety of Shouhuitongbian capsule after its marketing.
Health-related Quality of Life in Children With Kaposiform Hemangioendothelioma: A Case Control Study
Authors: Dai Shiyi , Yang Kaiying , Qiu Tong , Zhou Jiangyuan , Zhang Xuepeng , Chen Siyuan , Li Lizhi , Ji Yi 
Published: August 2020

Abstract
Background and objective: Kaposiform hemangioendothelioma (KHE) is a rare, aggressive and borderline vascular tumor mainly occurring in infants and children. The aim of this study was to determine the health-related quality of life (HRQOL) in children with KHE. Methods: A total of 91 children with KHE participated in this cross-sectional study. The HRQOL was assessed by the age-specific Pediatric Quality of Life Inventory Version 4.0 (PedsQL™ 4.0) Infant Scales, Family Information Form (FIF), Family Impact Module (FIM) and Generic Core Scales (GCS). For comparison, demographically matched healthy children were recruited as a control group. The main outcome measure of HRQOL was analyzed in the two groups. We determined related factors that influenced the HRQOL in children with KHE and their parents by using a stepwise multiple regression analysis. Results: Except for social and cognitive functioning, we found significant differences in the PedsQL™ 4.0 Infant Scales subscales between the patient group and healthy group ( P <0.05). In the PedsQL™ 4.0 GCS, all the subscales were significantly different between the patient group and the healthy group ( P <0.05). Additionally, in the ≤24 month age group, there were significant differences in the HRQOL between patients with and without Kasabach-Merritt phenomenon (KMP) in physical, physical symptoms, emotional and cognitive functioning ( P <0.05). In the >24 month age group, physical, emotional and social functioning were significantly different between the patients with and without activity dysfunction ( P <0.05). Conclusions: The findings presented here suggest that patients with KHE have a poor HRQOL. KMP and activity dysfunction are risk factors for poor HRQOL in patients with KHE. However, lesion size, lesion location and education level of the mother and father were not related to the HRQOL.
An investigation of honey bee virus prevalence in managed honey bees (Apis mellifera and Apis cerana) undergone colony losses: a case study in China
Authors: Deng Yanchun , Zhang Li , Jiang Xuejian , Yang Sa , Deng Shuai , Hou Chunsheng 
Published: August 2020

Abstract
Objective: In the absence of known clinical symptoms, viruses were considered to be the most probable key pathogens of honey bee. Therefore, the aim of this study was to investigate the prevalence and distribution of honey bee viruses in managed Apis mellifera and Apis cerana in China. Results: We conducted a screening of 9 honey bee viruses on A. mellifera and A. cerana samples collected from 54 apiaries from 13 provinces in China. We found that the types and numbers of viruses significantly differed between A. mellifera and A. cerana. Black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), Apis mellifera filamentous virus (AmFV) and Kakugo virus (DWV-C/KV) were the primary viruses found in A. mellifera colonies, whereas Chinese sacbrood bee virus (CSBV) and sacbrood bee virus (SBV) were the primary viruses found in A. cerana. The percentage infection of BQCV and CSBV were 84.6% and 61.6% in all detected samples. We first detected the occurrences of Varroa destructor virus-1(VDV-1, DWV-B) and DWV-C/KV in China but not ABPV in both A. mellifera and A. cerana.
The prognostic value of NLR for early death in secondary Hemophagocytic lymphohistiocytosis patients: an analysis of 92 patients
Authors: hu linhui , wu fan , Pan Ying , zhang jiakui , li qian , zhu weiwei , ding chen , meng ye , wang huiping , xiong shudao , zhai zhimin 
Published: August 2020

Abstract
Background: Hemophagocytic lymphohistiocytosis (HLH) a hyperinflammation disease and have high early mortality. The neutrophil-lymphocyte ratio (NLR) plays a prognostic role in various inflammation conditions. However, the role of NLR in HLH remains unknown.Results: 92 patients were collected from Sep 2014 to Dec 2019, the median age was 50 years (16-88) and 54 patients (58.6%) were male. 39 patients (42.3%) died during a 30-day follow-up. In addition, NLR was higher in nonsurvivor than survivor (P=0.005), higher NLR was correlated with older age (P=0.020), worse survival status (P=0.006), lower HB level (P=0.008), longer PT level (P=0.010), higher creatinine level (0.027) and higher IL-6 level (P=0.008)and in multivariate analysis, NLR (HR=2.508, 95%CI: 1.275-4.934), was indicated as an independent prognostic factor for early death. Conclusions: NLR was correlated with early death in HLH, suggest NLR could be used as a reference indicator for the monitoring and management of HLH.
Cutaneous Leishmaniasis and/or Dermal Leishmanoid in Himachal Pradesh (India)
Authors: Lata Suman , kumari Sandhya , Das Ram , Pasi Shweta , Dhiman Ramesh C 
Published: August 2020

Abstract
Background: Visceral Leishmaniasis (VL) is in elimination phase in India while cases of Cutaneous Leishmaniasis (CL) are spreading to new foci in different parts of the country. In Himachal Pradesh, a foci of CL have been reported along Satluj River, but the causative agent poses a dilemma. To ascertain the Leishmania species from CL cases from Shimla, Kullu and Kinnaur districts of Himachal Pradesh, the present study was undertaken. Methods: A total of 28 CL patients registered in Department of Dermatology, Indira Gandhi Medical College (IGMC) and Hospital Shimla in 2018, were tested by rk39. Barring 16 cases undergoing treatment, 12 fresh cases were subjected to microscopic detection of Leishmania parasite, PCR and sequencing. Skin biopsies of 3-4 mm diameter were taken in culture medium and in formalin under anesthetic and sterile conditions from the border of the lesions. Imprints were prepared for the detection of Leishmania amastigotes. Biopsy samples were inoculated into different culture media (M199, RPMI 1640, and NNN) and were incubated at 22-24°C. Cultures were examined microscopically for the growth of promastigotes up to four weeks. Polymerase chain reaction (PCR) was performed to characterize leishmania parasite species.Results: Of 28 patients, one patient was found positive for by rK39 dipstick test. One imprint was found positive for leishmania amastigotes. Twelve biopsy DNA samples were subjected to PCR for Leishmania kDNA, of which all the 12 were found positive ITS1 Leishmania specific set of primers while eight were found positive with JW11/12 lesihmania species specific set of primers. Identification of Leishmania species was confirmed by PCR-RFLP and sequencing method. Of 12 Leishmania positive samples, six were identified as L. donovani, three L. tropica, two L. major and one remained unidentified.Conclusion: The detection of L. donovani from cutaneous leishmniasis patients is a significant finding leading towards existence of atypical leishmaniasis in Himachal Pradesh.
Transfusion-naïve Thalassemia Pediatric Patients have Higher Incidence of Asthma: A Nationwide Population-based Retrospective Cohort Study
Authors: Hsieh Hsin-Yi , Huang Lin-Chi , Yu Hong-Ren , Kuo Kuang-Che , Lee Yi-Chen , Su Chung-Hao , Yang Yao-Hsu , Sheen Jiunn-Ming 
Published: August 2020

Abstract
Background: Few studies have studied the association between asthma and pediatric transfusion naïve thalassemia. Methods: A nationwide population-based retrospective cohort study was conducted using medical records of selected children from the 2010 Registry for Beneficiaries of the Taiwan National Health Insurance Research Database, with a follow-up period extending to the end of 2013. One thalassemia patient was matched with four control patients without thalassemia according to year of birth, sex, and the propensity score model based on comorbidities. Results: In this study, 794 patients with thalassemia and 3176 controls were included. Transfusion-naïve thalassemia children had higher rates to develop asthma (41.03 vs 36.69 per 1000 person-y; P = 0.032) than the non-thalassemia controls with an adjusted hazard ratio of 1.16 (95% confidence interval (CI) = 1.01–1.33). Boys in the thalassemia cohort had a significantly higher adjusted hazard ratio of asthma than those in the non-thalassemia cohort (adjusted hazard ratio = 1.20, 95% CI = 1.02–1.42). In thalassemic patients without atopic dermatitis, the risk of asthma was 1.25-fold higher than in the non-thalassemic ones (95% CI=1.07–1.47). Conclusions: Transfusion-naïve thalassemia children are more likely to have asthma. We need to pay more attention to it in order to diagnosis asthma earlier and to prevent asthma attacked earlier.
Decrease of HC and Hypoproteinemia: Possible Predictors of Complication of Neonatal Sepsis in a Developing Country?
Authors: Cai Na , Fan Wenting , Tao Min , Liao Wei 
Published: August 2020

Abstract
Background: The main purpose of our study was to determine the predictors of the complications of neonatal sepsis. Methods: This was a retrospective cohort study conducted in neonatal intensive care unit between June 2016 to February 2020. Neonates with late-onset sepsis (Age > 3 days old) were enrolled in this study. According to whether merged complication after sepsis, children were divided into complication group and non-complication group. The demographic data, perinatal conditions, blood cell count analysis, blood culture, hypoproteinemia within 1 week after the onset and treatment measures were compared. Results: A total of 87 infants with late-onset sepsis were enrolled in this study. (1) Significant differences were observed between the complication and non-complication group with regard to hemoglobin concentration (HC), decrease of HC, hypoproteinemia and red blood cell transfusion (P < 0.05). (2) Further comparison found that children with sepsis who had moderate and more severe anemia at the time of onset were more likely to have complications than those with mild or no anemia. (3) The results of binomial stepwise logistic regression suggested that significant decrease of HC (OR = 1.113, P = 0.000 < 0.05) and hypoproteinemia (OR = 3.953, P = 0.012 < 0.05) were independent risk factors for complication in infants with sepsis. (4) A receiver operating characteristic analysis showed that the AUC was 0.807 for decrease of HC.Conclusions: Significant decrease of HC and hypoproteinemia were independent risk factors for complication and may predict the occurrence of complication in infants with late-onset sepsis in the early stage.
Mature Cystic Teratoma of Ovary– a Case Report &amp; Review of Literature
Authors: Nayak Sunita , Dash Suren Prasad 
Published: August 2020

Abstract
Mature cystic teratoma or dermoid cyst is the most common germ cell tumour of the ovary. The incidence ranges from 5 to 25% of all ovarian neoplasms out of which 13.7% are bilateral. Teratomas usually occur in the ovaries but they may be seen in other sites known as extragonadal teratomas the incidence of which is only 0.4%. Teratomas are usually benign but they may turn out to be highly malignant. Torsion is known to be the most frequent complication of ovarian teratomas followed by autoamputation. Teratomas are classified as “Mature” and “Immature” by WHO. Teratomas show good prognosis specially the mature ones. Surgery and follow up remains the standard approach. We report a case of mature cystic teratoma in the right ovary of a 28 years old nulliparous woman.
IL-17 mediated macrophage polarization increased inflammatory damage in SWI-ALI models
Authors: Zhao Jiayi , Pu Jin , Zhang Rong , Fan Jian , Han Yiping , Xu Jianwen , Shang Yan 
Published: August 2020

Abstract
BackgroundSeawater inhalation induced acute lung injury (SWI-ALI) is the common accident in daily naval training. To investigate the mechanism of SWI-ALI will help to improve the treatment effect. Alveolar macrophages (AM) is the majority of alveolar, also paly the key role in SWI-ALI repair. IL-17 also paly the key role in the innate immunity process.MethodIn this study, we used seawater induced the ALI in mouse model. And the lungs and serum were exacted at D1, D3, D7 and D14. The AM polarization were tested by flow cytometry. The IL-17 concentration were tested by ELISA. Then the IL-17 function were confirmed by in vitro test. The mouse alveolar epithelial cell and mouse AM were co-cultured. The test compared the wound healing effect of MAE with and without IL-17.ResultThe AM switch into M1 and IL-17A increased were found after seawater dosing. And the IL-17a supplement attenuated wound healing of alveolar epithelial cells through improve the polarization of AM were confirmed in vitro model.ConclusionThe high IL-I7 micro-environment will increased the inflammatory damage through induced macrophage polarization in acute lung injury. The IL-17 antagonists have the potential to increase clinical effect in SWI-ALI treatment.