bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of 7,8-Dihydroxyflavone on Lipid Isoprenoid and Rho Protein Levels in Brains of Aged C57BL/6 Mice
Authors: Ötzkan Sarah , Muller Walter E. , Gibson Wood W. , Eckert Gunter P. 
Published: December 2020

AbstractSynaptic impairment may be the main cause of cognitive dysfunction in brain aging that is probably due to a reduction in synaptic contact between the axonal buttons and dendritic spines. Rho proteins including the small GTPase Rac1 have become key regulators of neuronal morphogenesis that supports synaptic plasticity. Small Rho- and Ras-GTPases are post-translationally modified by the isoprenoids geranylgeranyl pyrophosphate (GGPP) and farnesyl pyrophosphate (FPP), respectively. For all GTPases, anchoring in the plasma membrane is essential for their activation by guanine nucleotide exchange factors (GEFs). Rac1-specific GEFs include the protein T lymphoma invasion and metastasis 1 (Tiam1). Tiam1 interacts with the TrkB receptor to mediate the brain-derived neurotrophic factor (BDNF)-induced activation of Rac1, resulting in cytoskeletal rearrangement and changes in cellular morphology. The flavonoid 7,8-dihydroxyflavone (7,8-DHF) acts as a highly affine-selective TrkB receptor agonist and causes the dimerization and autophosphorylation of the TrkB receptor and thus the activation of downstream signaling pathways. In the current study, we investigated the effects of 7,8-DHF on cerebral lipid isoprenoid and Rho protein levels in male C57BL/6 mice aged 3 and 23 months. Aged mice were daily treated with 100 mg/kg b.w. 7,8-DHF by oral gavage for 21 days. FPP, GGPP, and cholesterol levels were determined in brain tissue. In the same tissue, the protein content of Tiam1 and TrkB in was measured. The cellular localization of the small Rho-GTPase Rac1 and small Rab-GTPase Rab3A was studied in total brain homogenates and membrane preparations. We report the novel finding that 7,8-DHF restored levels of the Rho proteins Rac1 and Rab3A in membrane preparations isolated from brains of treated aged mice. The selective TrkB agonist 7,8-DHF did not affect BDNF and TrkB levels, but restored Tiam1 levels that were found to be reduced in brains of aged mice. FPP, GGPP, and cholesterol levels were significantly elevated in brains of aged mice but not changed by 7,8-DHF treatment. Hence, 7,8-DHF may be useful as pharmacological tool to treat age-related cognitive dysfunction although the underlying mechanisms need to be elucidated in detail.
Efficacy of Magnetic Sphincter Augmentation Across the Spectrum of GERD Disease Severity
Authors: Schwameis Katrin , Ayazi Shahin , Zheng Ping , Grubic Andrew D. , Salvitti Madison , Hoppo Toshitaka , Jobe Blair A. 
Published: December 2020

BACKGROUND:
The performance and durability of various types of fundoplication are variable when stratified by disease severity. To date, magnetic sphincter augmentation (MSA) has not been evaluated in this context. We designed this study to determine the efficacy of MSA in the treatment of severe GERD.

STUDY DESIGN:
Guided by previous studies, a DeMeester score (DMS) ≥ 50 was used as a cutoff point to define severe reflux disease. Subjects were divided into 2 groups using this cutoff, and outcomes of severe cases were compared with those with less severe disease (DMS < 50).

RESULTS:
A total of 334 patients underwent MSA. Patients with severe disease had a higher mean preoperative DMS compared with those with mild to moderate GERD (79.2 [53.2] vs 22.8 [13.7], p < 0.0001). At a mean postoperative follow-up of 13.6 (10.4) months, there was no difference between the mean GERD Health-Related Quality of Life (HRQL) total scores in patients with severe disease compared with those with less severe GERD (8.8 [10] vs 9.2 [10.8], p = 0.9204). Postoperative mean DMS was not different between groups (17.3[23.0] vs 14.1[33.9], p = 0.71), and there was no difference in the prevalence of esophagitis (p = 0.52). Patients with severe disease were less likely to be free from use of proton pump inhibitors after surgery (85% vs 93.1%, p = 0.041). There were similar rates of postoperative dysphagia (10% vs 14%, p = 0.42) and need for device removal (3% vs 5%, p = 0.7463).

CONCLUSIONS:
MSA is an effective treatment in patients with severe GERD and leads to significant clinical improvement across the spectrum of disease severity, with few objective outcomes being superior in patients with mild-to-moderate reflux disease.
Holland House: Architecture in an Elite Society
Authors: Cooper Nicholas 
Published: December 2020

AbstractHolland House in Kensington, then on the western fringes of London, was built in 1603–05. Its builder, Walter Cope (?1553–1614), rose as an administrator by exploiting his close association with leading statesmen. The article suggests that the building's remarkable plan and appearance may have had more complex sources than previously proposed. The house was inherited by Cope's son-in-law, the first Earl of Holland (1590–1649), and newly discovered documents throw light on the extensive architectural and decorative changes carried out for him between 1630 and 1640. It is argued that, for both owners, the house was instrumental in their social and political ambitions.
Discovering the Byzantine Art of Building: Lectures at the RIBA, the Royal Academy and the London Architectural Society, 1843–58
Authors: Karydis Nikolaos 
Published: December 2020

ABSTRACTAlthough British architects played a major role in the rediscovery of the Byzantine monuments of Greece in the late nineteenth and early twentieth centuries, earlier interest in the subject has remained obscure. Four lectures, read at the Royal Institute of British Architects, the Royal Academy and the London Architectural Society from 1843 to 1857, reflect a lively interest in Byzantine church architecture in the mid-nineteenth century. Delivered by Charles Robert Cockerell (1843), Edwin Nash (1847), Thomas Leverton Donaldson (1853) and John Louis Petit (1858), these lectures constitute some of the earliest attempts in England to explore both well-known monuments such as Hagia Sophia and lesser-known churches in Greece, Turkey and elsewhere. The manuscript records of these lectures show that influential British architects were not only familiar with Byzantine monuments, but were also able to look at them from the viewpoint of the designer and the builder. Emphasising the potential of Byzantine architecture to inform new design, they paved the way for the Byzantine revival, half a century later, and for the systematic investigation of Byzantine architecture from the late nineteenth century onwards.
Gouvernementalité extractive et autodétermination au Canada. Écosystèmes normatifs et charge critique de l’inter-normativité
Authors: Roy Grégoire Étienne 
Published: December 2020

RésuméLa relativisation de la souveraineté est à la fois au cœur de la critique du pluralisme juridique et le résultat concret de la globalisation néolibérale. Cet article propose ainsi la notion d’écosystème normatif comme milieu pour la structuration des communautés politiques. Il énonce trois hypothèses concernant la configuration de l’écosystème normatif extractif canadien : 1) des enjeux constitutionnels y sont traités par le biais de relations contractuelles entre entreprises et communautés autochtones; 2) la responsabilité sociale des entreprises y fait office de régime de véridiction; et 3) l’impératif d’efficacité y prend préséance sur les visées téléologiques en présence. Ces hypothèses configurent une gouvernementalité moralisante peu amène aux communautés politiques. Cet article propose donc de renouveler la charge critique du pluralisme juridique en insistant sur l’hétéronomie de la norme comme condition de possibilité de la liberté politique.
Life as an Australian Aboriginal and Torres Strait Islander Male Prisoner: Poems of Grief, Trauma, Hope, and Resistance
Authors: Marchetti Elena , Bargallie Debbie 
Published: December 2020

AbstractFor Australia’s Aboriginal and Torres Strait Islander people, writing is predominantly about articulating their cultural belonging and identity. Published creative writing, which is a relatively new art form among Aboriginal and Torres Strait Islander prisoners, has not been used as an outlet to the same extent as other forms of art. This is, however, changing as more Aboriginal and Torres Strait Islander rappers and story-writers emerge, and as creative writing is used as a way to express Aboriginal and Torres Strait Islander empowerment and resistance against discriminatory and oppressive government policies. This article explores the use of poetry and stories written by Aboriginal and Torres Strait Islander male prisoners in a correctional facility located in southern New South Wales, Australia, to understand how justice is perceived by people who are (and have been) surrounded by hardships, discrimination, racism, and grief over the loss of their culture, families, and freedom.
Utilisation of early intervention services in infants with congenital heart disease following open-heart surgery
Authors: West Caroline , Yu Sunkyung , Lowery Ray , Goldberg Caren S. , Uzark Karen 
Published: December 2020

AbstractObjective:To examine the use of early intervention services in infants with CHD after open-heart surgery and identify factors associated with receipt of services.Study design:Surveys were administered to caregivers of infants who underwent open-heart surgery before 1 year of age at a single institution between July, 2017 and July, 2018. Information regarding the infant’s use of early intervention services and the caregiver’s experience with the programme was obtained. Clinical data were retrieved from the medical record review. Logistic regression identified factors associated with receipt of services.Results:The study included 158 eligible infants. Ninety-eight caregivers (62%) completed the surveys. Of those surveyed, 53.1% of infants were currently or previously enrolled in early intervention services. Infants most frequently received physical therapy (76.9%). The majority of caregivers found services to be moderately/very helpful (92.3%) and sufficient for their child (76.9%). In the univariate analysis, single-ventricle disease, known syndrome/genetic abnormality, extracardiac anomaly, and longer intensive care and hospital length of stay were associated with receipt of services. Single-ventricle disease (p = 0.004) and known syndrome/genetic abnormality (p < 0.0001) remained independently associated with receipt of services in the multivariable analysis.Conclusion:Amongst infants at risk for neurodevelopmental deficits, approximately half received services after open-heart surgery. Caregivers expressed satisfaction with the programme. While infants with single-ventricle disease and a known syndrome/genetic abnormality were more likely to receive early intervention services, many at-risk infants with CHD failed to receive services. Further research is needed to identify barriers to early intervention services and promote developmental outcomes.
Apical hypertrophic cardiomyopathy with subendocardial late gadolinium enhancement in an adolescent
Authors: Boroni Grazioli Simona , Hitz Marc-Philip , Voges Inga 
Published: December 2020

AbstractA 17-year-old boy with a history of dyspnea attacks and chest pain was referred to our paediatric cardiology department. Electrocardiogram at presentation showed T-wave inversion in the inferior leads. Cardiovascular magnetic resonance imaging revealed the rare diagnosis of apical hypertrophic cardiomyopathy with subendocardial late gadolinium enhancement, missed by echocardiography.
Juvenile Eastern Oysters More Resilient to Extreme Ocean Acidification than Their Mud Crab Predators
Authors: Dodd L. F. , Grabowski J. H. , Piehler M. F. , Westfield I. , Ries Justin B. 
Published: December 2020

AbstractOcean acidification is predicted to impair marine calcifiers' abilities to produce shells and skeletons. We conducted laboratory experiments investigating the impacts of CO2‐induced ocean acidification (pCO2 = 478–519, 734–835, 8,980–9,567; Ωcalcite = 7.3–5.7, 5.6–4.3, 0.6–0.7) on calcification rates of two estuarine calcifiers involved in a classic predator‐prey model system: adultPanopeus herbstii(Atlantic mud crab) and juvenileCrassostrea virginica(eastern oyster). Both oyster and crab calcification rates significantly decreased at the highestpCO2level. Notably, however, oysters maintained positive net calcification rates in the highest pCO2treatment that was undersaturated with respect to calcite, while mud crabs exhibited net dissolution (i.e., net loss of shell mass) in calcite‐undersaturated conditions. Secondary electron imaging of oyster shells revealed minor microstructural alterations in the moderate‐pCO2treatment, and major microstructural and macrostructural changes (including shell dissolution, delamination of periostracum) in the high‐pCO2treatment. These results underscore the threat that ocean acidification poses for marine organisms that produce calcium carbonate shells, illustrate the strong biological control that some marine calcifiers exert over their shell‐building process, and shows that ocean acidification differentially impacts the crab and oyster species involved in this classical predator‐prey model system.
Nonlinear Modeling of Subsidence From a Decade of InSAR Time Series
Authors: Park Seo‐Woo , Hong Sang‐Hoon 
Published: December 2020

AbstractWe present a model of ground subsidence due to soil consolidation within coastal reclaimed land constructed in early 2001, in Busan, South Korea. We estimate 42 cm of maximum subsidence during the period of interferometric synthetic aperture radar observations, and a maximum of 1 m or more subsidence, since the end of the construction phase of the reclamation project. A hyperbolic model was used to relate the three independent time series and to estimate the nonlinear behavior of subsidence. The model estimates a maximum subsidence rate and acceleration of −5.7 cm/year and 1.1 cm/year2, respectively. The decomposition of vertical and east‐west horizontal motion using the Sentinel‐1 dataset indicates relatively weak partial horizontal displacement as well as substantial vertical deformation overall. The long‐term time series analysis indicates ongoing ground subsidence, although almost two decades have passed since the completion of reclamation.
Mirror Instabilities in the Inner Magnetosphere and Their Potential for Localized ULF Wave Generation
Authors: Cooper M. B. , Gerrard A. J. , Lanzerotti L. J. , Soto‐Chavez A. R. , Kim H. , Kuzichev I. V. , Goodwin L. V. 
Published: December 2020

AbstractResults from the NASA Van Allen Probes mission indicate extensive observations of mirror/drift‐mirror (M/D‐M hereafter) unstable plasma regions in the night‐side inner magnetosphere. Said plasmas lie on the threshold between the kinetic and frozen‐in plasma regimes and have favorable conditions for the formation of M/D‐M modes and subsequent ultralow frequency (ULF) wave signatures in the surrounding plasma. We present the results of a climatological analysis of plasma‐γ (anisotropy measure) and total plasma‐β (ratio of particle to magnetic field pressure) in regard to the satisfaction of instability conditions on said M/D‐M modes under bi‐Maxwellian distribution assumption, and ascertain the most likely region for such plasmas to occur. Our results indicate a strong preference for the premidnight sector of the night‐side magnetosphere, with events ranging in time scales from half a minute (roughly 200 km in scale size) to several hours (multiple Earth radii). The statistical distribution of these plasma regions explicitly identifies the source region of “storm time Pc5 ULF waves” and suggests an alternative mechanism for their generation in the night‐side inner magnetosphere.
Assessing the Influence of Shoreline Adaptation on Tidal Hydrodynamics: The Role of Shoreline Typologies
Authors: Hummel Michelle A. , Stacey Mark T. 
Published: December 2020

AbstractIn coastal embayments, tidal dynamics are influenced by mean sea level and shoreline configuration. As sea levels rise and coastal communities modify their shorelines to mitigate future flooding, spatial patterns of inundation, tidal amplitude, and tidal phase will change in response. Here we apply a hydrodynamic model to systematically investigate the effect of local‐scale, spatially refined shoreline protection on regional tidal dynamics and peak water levels in San Francisco Bay, California. We find that individual shoreline protection scenarios produce relatively small changes in tidal amplitudes at 50 and 100 cm of sea‐level rise but can result in amplification of the M2 tide by up to 40% at 200 cm of sea‐level rise. Protection of shorelines with gradually sloping topography and space for floodwater accommodation generally has the largest influence on tidal response, both locally and regionally. In the northern part of San Francisco Bay, tides down‐estuary of protected shorelines move closer to a standing wave, while up‐estuary tides become more progressive. In the southern part of the bay, which is already close to a standing wave, effects are more localized and variable. Overall, the tidal response to shoreline protection is a function of both the geographic location within the embayment as well as the geomorphic characteristics of the local landscape that is protected. Understanding the relative influence of local shoreline modifications on tidal dynamics under a range of sea‐level rise scenarios is critical to developing effective shoreline adaptation alternatives that maximize flood mitigation.
Combination of anti-PD-1 antibody with P-GEMOX as a potentially effective immunochemotherapy for advanced natural killer/T cell lymphoma
Authors: Cai Jun , Liu Panpan , Huang Huiqiang , Li Yajun , Ma Shuyun , Zhou Hui , Tian Xiaopeng , Zhang Yuchen , Gao Yan , Xia Yi , Zhang Xuanye , Yang Hang , Li Lirong , Cai Qingqing 
Published: December 2020

AbstractAdvanced natural killer/T cell lymphoma (NKTL) has demonstrated poor prognosis with currently available therapies. Here, we report the efficacy of anti-programmed death 1 (PD-1) antibody with the P-GEMOX (pegaspargase, gemcitabine, and oxaliplatin) regimen in advanced NKTL. Nine patients underwent six 21-day cycles of anti-PD-1 antibody (day 1), pegaspargase 2000 U/m2 (day 1), gemcitabine 1 g/m2 (days 1 and 8) and oxaliplatin 130 mg/m2 (day 1), followed by anti-PD-1 antibody maintenance every 3 weeks. Programmed death-ligand 1 (PD-L1) expression and genetic alterations were determined in paraffin-embedded pretreatment tissue samples using immunohistochemistry and next-generation sequencing (NGS) analysis. Responses were assessed using 18F-fluorodeoxyglucose positron emission tomography (18FDG-PET) and computed tomography or magnetic resonance imaging. Eight patients exhibited significant responses, comprising of seven complete remissions and one partial remission (overall response rate: 88.9%). After a median follow-up of 10.6 months, 6/9 patients (66.7%) remained in complete remission. The most common grade 3/4 adverse events were anemia (33.3%), neutropenia (33.3%), and thrombocytopenia (33.3%); all of which were manageable and resolved. Immunochemotherapy produced a high response rate in patients with positive PD-L1 expression (5/6, 83.3%). NGS analysis suggested that STAT3/JAK3/PD-L1 alterations and ARID1A mutation were associated with immunochemotherapy efficacy. Mutation in DDX3X and alteration in epigenetic modifiers of KMT2D, TET2, and BCORL1 might indicate a poor response to immunochemotherapy. In conclusion, the anti-PD-1 antibody plus P-GEMOX regimen demonstrated promising efficacy in advanced NKTL. PD-L1 expression combined with specific genetic alterations could be used as potential biomarkers to predict therapeutic responses to immunochemotherapy.
Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types
Authors: Barclay R. A. , Akhrymuk I. , Patnaik A. , Callahan V. , Lehman C. , Andersen P. , Barbero R. , Barksdale S. , Dunlap R. , Goldfarb D. , Jones-Roe T. , Kelly R. , Kim B. , Miao S. , Munns A. , Munns D. , Patel S. , Porter E. , Ramsey R. , Sahoo S. , Swahn O. , Warsh J. , Kehn-Hall K. , Lepene B. 
Published: December 2020

AbstractHere we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from contrived transport medium and saliva samples using affinity-capture magnetic hydrogel particles. We demonstrate that the method concentrates virus from 1 mL samples prior to RNA extraction, substantially improving detection of virus using real-time RT-PCR across a range of viral titers (100–1,000,000 viral copies/mL) and enabling detection of virus using the 2019 nCoV CDC EUA Kit down to 100 viral copies/mL. This method is compatible with commercially available nucleic acid extraction kits (i.e., from Qiagen) and a simple heat and detergent method that extracts viral RNA directly off the particle, allowing a sample processing time of 10 min. We furthermore tested our method in transport medium diagnostic remnant samples that previously had been tested for SARS-CoV-2, showing that our method not only correctly identified all positive samples but also substantially improved detection of the virus in low viral load samples. The average improvement in cycle threshold value across all viral titers tested was 3.1. Finally, we illustrate that our method could potentially be used to enable pooled testing, as we observed considerable improvement in the detection of SARS-CoV-2 RNA from sample volumes of up to 10 mL.
Continuous lengthening potential after four years of magnetically controlled spinal deformity correction in children with spinal muscular atrophy
Authors: Lorenz Heiko M. , Hecker Marina M. , Braunschweig Lena , Badwan Batoul , Tsaknakis Konstantinos , Hell Anna K. 
Published: December 2020

AbstractMagnetically controlled growing rods (MCGR) are commonly implanted for the treatment of early-onset scoliosis. While most authors report favorable short-term results, little is known about long-term deformity correction. This prospective cohort study assesses spinal deformity control in a homogeneous spinal muscular atrophy (SMA) patient group treated with MCGR implants, a standardized lengthening protocol and a minimum follow-up of four years. 17 SMA patients with progressive scoliosis were treated with MCGR implanted parallel to the spine with rib-to-pelvis fixation. Radiologic measurements were performed before and after MCGR implantation and during external lengthening procedures. These included measurements of the scoliotic curve, kyphosis, lordosis, pelvic obliquity and the spinal length. Additional clinical data of the complications were also analyzed. 17 children (mean age 7.4 years) were surgically treated and underwent a total of 376 lengthenings. Complication rates were 3.5% in respect to all interventions or 41% of the patients had complications during 3.5% of the lengthening sessions. The initial implantation significantly reduced the main scoliotic curve by 59%, with the correction remaining constant throughout the follow-up. Pelvic obliquity was also significantly and permanently corrected by 72%, whereas kyphosis and lordosis were not influenced. The spinal length could be significantly increased mostly during the first year of treatment. Bilateral implantation of MCGRs for correction of spinal deformity in children with SMA showed no decrease of the lengthening potential during a four-year follow-up. Therefore, the previously described ‘law of diminishing returns’ could not be applied to this patient population.Level of Evidence/Clinical relevance: Therapeutic Level IV.