bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integration of Islet/Beta-Cell Transplants with Host Tissue Using Biomaterial Platforms
Authors: Clough Daniel W , King Jessica L , Li Feiran , Shea Lonnie D 
Published: September 2020

AbstractCell-based therapies are emerging for type I diabetes mellitus (T1D), an autoimmune disease characterized by the destruction of insulin-producing pancreatic β-cells, as a means to provide long-term restoration of glycemic control. Biomaterial scaffolds provide an opportunity to enhance the manufacturing and transplantation of islets or stem cell–derived β-cells. In contrast to encapsulation strategies that prevent host contact with the graft, recent approaches aim to integrate the transplant with the host to facilitate glucose sensing and insulin distribution, while also needing to modulate the immune response. Scaffolds can provide a supportive niche for cells either during the manufacturing process or following transplantation at extrahepatic sites. Scaffolds are being functionalized to deliver oxygen, angiogenic, anti-inflammatory, or trophic factors, and may facilitate cotransplantation of cells that can enhance engraftment or modulate immune responses. This local engineering of the transplant environment can complement systemic approaches for maximizing β-cell function or modulating immune responses leading to rejection. This review discusses the various scaffold platforms and design parameters that have been identified for the manufacture of human pluripotent stem cell–derived β-cells, and the transplantation of islets/β-cells to maintain normal blood glucose levels.
Paraventricular Dynorphin A Neurons Mediate LH Pulse Suppression Induced by Hindbrain Glucoprivation in Female Rats
Authors: Tsuchida Hitomi , Mostari Parvin , Yamada Koki , Miyazaki Sae , Enomoto Yuki , Inoue Naoko , Uenoyama Yoshihisa , Tsukamura Hiroko 
Published: September 2020

Abstract
Malnutrition suppresses reproductive functions in mammals, which is considered to be mostly due to the inhibition of pulsatile gonadotropin-releasing hormone (GnRH)/gonadotropin secretion. Accumulating evidence suggests that kisspeptin neurons in the arcuate nucleus (ARC) play a critical role in the regulation of pulsatile GnRH/gonadotropin release. The present study aimed to examine if the hypothalamic dynorphin A (Dyn) neurons mediate the suppression of GnRH/luteinizing hormone (LH) pulses during malnutrition. Ovariectomized rats treated with a negative feedback level of estradiol-17β-treated (OVX+E2) were administered with intravenous (iv) or fourth cerebroventricle (4V) 2-deoxy-D-glucose (2DG), an inhibitor of glucose utilization, to serve as a malnutrition model. Central administration of a Dyn receptor antagonist blocked the iv- or 4V-2DG-induced suppression of LH pulses in OVX+E2 rats. The 4V 2DG administration significantly increased the number of Pdyn (Dyn gene)-positive cells co-expressing fos in the paraventricular nucleus (PVN), but not in the ARC and supraoptic nucleus (SON), and the iv 2DG treatment significantly increased the number of fos and Pdyn-co-expressing cells in the PVN and SON, but decreased it in the ARC. The E2 treatment significantly increased Pdyn expression in the PVN, but not in the ARC and SON. Double in situ hybridization for Kiss1 (kisspeptin gene) and Oprk1 (Dyn receptor gene) revealed that around 60% of ARC Kiss1-expressing cells co-expressed Oprk1. These results suggest that the PVN Dyn neurons, at least in part, mediate LH pulse suppression induced by the hindbrain or peripheral glucoprivation, and Dyn neurons may directly suppress the ARC kisspeptin neurons in female rats.
Differential Effects of Voclosporin and Tacrolimus on Insulin Secretion From Human Islets
Authors: Kolic Jelena , Beet Leanne , Overby Peter , Cen Haoning Howard , Panzhinskiy Evgeniy , Ure Daren R , Cross Jennifer L , Huizinga Robert B , Johnson James D 
Published: September 2020

Abstract
The incidence of new onset diabetes after transplant (NODAT) has increased over the past decade, likely due to calcineurin inhibitor–based immunosuppressants, including tacrolimus (TAC) and cyclosporin. Voclosporin (VCS), a next-generation calcineurin inhibitor, is reported to cause fewer incidences of NODAT but the reason is unclear. While calcineurin signaling plays important roles in pancreatic β-cell survival, proliferation, and function, its effects on human β-cells remain understudied. In particular, we do not understand why some calcineurin inhibitors have more profound effects on the incidence of NODAT. We compared the effects of TAC and VCS on the dynamics of insulin secretory function, programmed cell death rate, and the transcriptomic profile of human islets. We studied 2 clinically relevant doses of TAC (10 ng/mL, 30 ng/mL) and VCS (20 ng/mL, 60 ng/mL), meant to approximate the clinical trough and peak concentrations. TAC, but not VCS, caused a significant impairment of 15 mM glucose-stimulated and 30 mM KCl-stimulated insulin secretion. This points to molecular defects in the distal stages of exocytosis after voltage-gated Ca2+ entry. No significant effects on islet cell survival or total insulin content were identified. RNA sequencing showed that TAC significantly decreased the expression of 17 genes, including direct and indirect regulators of exocytosis (SYT16, TBC1D30, PCK1, SMOC1, SYT5, PDK4, and CREM), whereas VCS has less broad, and milder, effects on gene expression. Clinically relevant doses of TAC, but not VCS, directly inhibit insulin secretion from human islets, likely via transcriptional control of exocytosis machinery.
Myosin 1b flattens and prunes branched actin filaments
Authors: Pernier Julien , Morchain Antoine , Caorsi Valentina , Bertin Aurélie , Bousquet Hugo , Bassereau Patricia , Coudrier Evelyne 
Published: September 2020

ABSTRACT
Motile and morphological cellular processes require a spatially and temporally coordinated branched actin network that is controlled by the activity of various regulatory proteins, including the Arp2/3 complex, profilin, cofilin and tropomyosin. We have previously reported that myosin 1b regulates the density of the actin network in the growth cone. Here, by performing in vitro F-actin gliding assays and total internal reflection fluorescence (TIRF) microscopy, we show that this molecular motor flattens (reduces the branch angle) in the Arp2/3-dependent actin branches, resulting in them breaking, and reduces the probability of new branches forming. This experiment reveals that myosin 1b can produce force sufficient enough to break up the Arp2/3-mediated actin junction. Together with the former in vivo studies, this work emphasizes the essential role played by myosins in the architecture and dynamics of actin networks in different cellular regions.
This article has an associated First Person interview with the first author of the paper.
Transcutaneous bilirubin level to predict hyperbilirubinemia in preterm neonates
Authors: Rahmawati Dewi , Sampurna Mahendra Tri Arif , Etika Risa , Utomo Martono Tri , Bos Arend F. 
Published: September 2020

Background: Hyperbilirubinemia is common in neonates, with higher prevalence among preterm neonates, which can lead to severe hyperbilirubinemia. Assessment of total serum bilirubin (TSB) and the use of a transcutaneous bilirubinometry (TcB) are existing methods that identify and predict hyperbilirubinemia. This study aimed to determine TcB cut-off values during the first day for preterm neonates to predict hyperbilirubinemia at 48 and 72 hours. Methods: This cohort study was conducted at Dr. Soetomo General Hospital from September 2018 to January 2019 a total of 90 neonates born ≤35 weeks. They were divided into two groups (Group I: 1000-1500 grams; Group II: 1501-2000 grams). The bilirubin levels were measured on the sternum using TcB at the ages of 12, 24, and 72 hours. TSB measurements were taken on the third day or if the TcB level reached phototherapy threshold ± 1.24 mg/dL and if TcB showed abnormal results (Group I: 5.76-8.24 mg/dL; Group II: 8.76-11.24 mg/dL). Hyperbilirubinemia was defined as TSB ≥7 mg/dL for Group I and >10 mg/dL for Group II. Results: In total, 38 Group I neonates and 48 Group II neonates were observed. Almost half of the neonates in Group I (45%) suffered from hyperbilirubinemia at the age of 48 hours, along with 46% of Group II at 72 hours. The best 24-hour-old TcB cut-off values to predict hyperbilirubinemia at 48 hours were calculated to be 4.5 mg/dL for Group I and 5.8 mg/dL for Group II. The determined 24-hour-old TcB value to predict hyperbilirubinemia at 72 hours was 5.15 mg/dL for Group II. Conclusion: TcB values in the early days of life can be used as hyperbilirubinemia predictors on the following days for preterm neonates. Close monitoring should be managed for those with TcB values higher than the calculated cut-off values.
Feasibility of phenytoin as a paramedic-led second-line anti-epileptic drug
Authors: Morrison Luke 
Published: September 2020

Background: Convulsive status epilepticus (CSE) is a medical emergency that is commonly encountered in the prehospital setting. In almost all prehospital settings, treatment is limited to benzodiazepines even though the standard of care in emergency departments includes second-line agents such as phenytoin. Methods: A literature search was conducted using PubMed and Google Scholar using the search terms ‘phenytoin’, ‘seizure’ or ‘convulsive’ and ‘prehospital’, ‘EMS’ or ‘ambulance’ or ‘emergency department’. Five articles were analysed and a narrative review formed. Results: Phenytoin is an effective and commonly used second-line anti-epileptic agent but there is a distinct lack of evidence on prehospital phenytoin. Phasing the introduction of phenytoin into practice while simultaneously running a well-designed research trial could provide data for prehospital providers and the wider health community. Conclusion: Management of CSE will continue to present challenges to prehospital providers. Promoting the introduction of phenytoin to select patients, administered by advanced clinicians, could be an excellent opportunity to generate much-needed clinical data and potentially reduce morbidity and mortality in CSE.
Prehospital thrombolysis for STEMI where PPCI delays are unavoidable
Authors: Lashwood David 
Published: September 2020

Background: Primary percutaneous coronary intervention (PPCI) is the gold standard for treating patients experiencing ST-elevation acute myocardial infarction (STEMI). More than 30 000 patients experience cardiac arrest out of a hospital setting in the UK every year and may be some distance from a PPCI facility. Aims: To analyse and consider if a better outcome could be achieved for patients if PPCI was an adjunct to thrombolytic therapy, where delays of ≥60 minutes are inevitable or unavoidable. Methods: The current review examined a range of articles, research materials and databases. Results: Some studies suggested the use of prehospital thrombolysis while others compared the effectiveness of drug-eluting stents. While the ‘gold standard’ for the treatment of patients experiencing a myocardial infarction is still PPCI, several factors can delay patients from receiving this treatment at an appropriate facility within the recommended time frame. Conclusion: Patients may not be able to access PPCI within 60, 90 or 120 minutes for reasons including increasing urbanisation, population growth and NHS hospital funding cuts. If the PPCI unit is some distance away, ambulance crews could start thrombolysis treatment and transmit clinical findings to a specialist cardiologist in the PPCI facility, or stop at a local hospital that could provide thrombolysis.
Health behaviours in ambulance workers
Authors: Hutchinson Lucy Catherine , Forshaw Mark J , Poole Helen 
Published: September 2020

Introduction: Awareness is increasing that health behaviours, which are a part of a person's lifestyle, have significant effects on emotional and physical wellbeing. Ambulance workers are at a higher risk of poorer psychological health outcomes than the general population. This begs the question whether lifestyle could play a role in emotional and physical health outcomes, which is an understudied area in this population. This paper reviews health behaviours in paramedics and assesses the impact they may have on their emotional and physical wellbeing. Methodology: PRISMA guidelines were adhered to and seven online bibliographic databases (MEDLINE, CINAHL, PsychArticles, PsychINFO, Web of Science, PubMed and Google Scholar) and reference lists of eligible articles were searched. Papers were systematically extracted and selected by title, then by abstract using inclusion and exclusion criteria. Findings: The papers included in this review (n=6) cover a range lifestyle factors (physical activity, smoking, alcohol use and sleep) that potentially affect wellbeing outcomes (weight/body mass index and post-traumatic stress symptoms) of ambulance workers across the Western world. They have various limitations. Conclusion: Ambulance workers engage in negative health behaviours that have some bearing on their emotional and physical wellbeing. Further research could explore the role of health behaviours and lifestyle in ambulance workers using validated measures. The findings could support the development of an evidence-based, occupation-specific intervention.
A checklist and conservation status of vascular plants in the Limestone forest of Metropolitan Ilocos Norte Watershed Forest Reserve, Northwestern Luzon, Philippines
Authors: Batuyong Mae Ann , CALARAMO MICHAEL A. , ALEJANDRO GRECEBIO JONATHAN D. 
Published: September 2020

Abstract. Batuyong MAR, Calaramo MA, Alejandro GJD. 2020. A checklist and conservation status of vascular plants in the Limestone forest of Metropolitan Ilocos Norte Watershed Forest Reserve, Northwestern Luzon, Philippines. Biodiversitas 21: 3969-3981. The Metropolitan Ilocos Norte Watershed Forest Reserve (MINWFR) is among the remaining intact limestone formations and a critical protected area in Northwestern Luzon. There have been few published floristic studies despite its undeniable rich biological importance. Therefore, this paper primarily aims to provide a preliminary checklist of vascular plants in MINWFR and their conservation status. Consequent field visits and surveys were made from April 2019 to March 2020. Results revealed a total of 173 species distributed in 140 genera belonging to 59 families. The most represented families are Rubiaceae, Fabaceae, Orchidaceae, and Malvaceae. This forest supports 49% or 28.65 % local endemics, five of which are narrow endemics, namely Cyanometra warburgii, Pyrostria triflora, Syzygium ilocanum, Thrixspermum nicolasiorum, and Antirhea microphylla. The latter, however, extends up to Ilocos Sur. Based on IUCN criteria and DENR records, a total of 18 species are threatened, one species is recorded as critically endangered, five endangered, nine vulnerable, three other threatened, two near threatened, 55 as least concern, and the rest are not evaluated. Other noteworthy species present in the area are the two dominant endangered species, Podocarpus costalis, and Podocarpus polystachyus. An interesting spinescent Rubiaceae species were recorded and currently under examination to establish its identity. This checklist serves as a basis to effectively manage this vulnerable area surrounded by human-induced disturbances and threats.
Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)
Authors: Gupta Yash Munnalal , TANASARNPAIBOON SUPATCHAREE , BUDDHACHAT KITTISAK , PEYACHOKNAGUL SURIN , INTHIM PHATTHARAPORN , HOMCHAN SOMJIT 
Published: September 2020

Abstract. Gupta YM, Tanasarnpaiboon S, Buddhachat K, Peyachoknagul S, Inthim P, Homchan S. 2020. Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae). Biodiversitas 21: 4094-4099. The house cricket, Acheta domesticus, is one of the species of crickets commonly found in Thailand. Insect breeders in Thailand prefer to breed house cricket as food due to its better taste and popularity among local people. Moreover, largescale breeding industries also breed house cricket to produce cricket-based edible products. Insect breeding industry is growing rapidly and requires primary precaution for sustainable production. To facilitate breeding system to maintain genetic variation in the captive population, we have sequenced the whole genome of A. domesticus to search for simple sequence repeats (SSRs) in order to develop polymorphic microsatellite markers for preliminary population genetic analysis. A total of 112,157 SSRs with primer pairs were identified in our analysis. Of these, 91 were randomly selected to check for amplification of microsatellite polymorphisms. From these, nine microsatellites were used to check genetic variation in forty-five individuals of A. domesticus from the Phitsanulok population (Thailand). These microsatellite markers also showed cross-amplification with other three species of edible crickets, specifically Gryllus bimaculatus, Gryllus testaceus, and Brachytrupes portentosus. The microsatellite markers presented herein will facilitate future population genetic analysis of A. domesticus populations. Moreover, the transferability of these makers would also enable researchers to conduct genetic studies for other closely related species.
Лабиринтами Матрицы: осваивая социальный конструкционизм
Authors: Подвойский Денис Глебович 
Published: September 2020

В настоящее время Департамент издательских программ ВЦИОМ завершает подготовку к публикации перевода книги социальных психологов Э. Лока и Т. Стронга «Social Constructionism: Sources and Stirrings in Theory and Practice». На русский язык книга выйдет под заголовком «Как устроена матрица? Социальное конструирование реальности: теория и практика». Журнал «Мониторинг общественного мнения» публикует вступительную статью к этому переводу, написанную специально для русскоязычного читателя. В представленном тексте содержится не только вводный комментарий к указанной книге, но и развернутый анализ комплекса «конструкционистских» и «конструктивистских» идей, оказывавших и продолжающих оказывать значительное влияние на развитие социальных и гуманитарных наук во всем мире.
«Пойду домой, домой это значит в подвал»: повседневные практики и стратегии выживания бездомных женщин
Authors: Кузинер Евгения Николаевна 
Published: September 2020

В статье рассматриваются повседневные практики и стратегии выживания бездомных женщин. Фокус на том, как проживают, переживают и справляются с ситуацией бездомности женщины, оказавшиеся без дома. Выделены две траектории попадания в ситуацию бездомности: утрата физического дома вследствие мошеннических операций с недвижимостью, пожара или других несчастных случаев и принуждение покинуть дом. Также рассматриваются три способа совладания с бездомностью: обращение за помощью в благотворительные организации; обращение за помощью к родственникам и друзьям; нормализация жизни на улице. В статье подчеркивается «невидимость» бездомных женщин и описываются причины женской бездомности и стратегии, которые они используют для выживания на улице.
Благодарность. Автор выражает благодарность своему научному руководителю Н.А. Нартовой, внесшей значительный вклад в написание магистерской диссертации, на основе которой написана данная статья, а также организациям и проводникам, обеспечившим доступ в поле, и анонимным рецензентам, чьи комментарии и замечания помогли улучшить и доработать статью.
Do Lawyers Need Economists? Review of Katja Langenbucher, Economic Transplants: On Lawmaking for Corporations and Capital Markets (Cambridge U. Press, 2017)
Authors: Avi-Yonah Reuven 
Published: September 2020

Abstract
Katja Langenbucher’s outstanding book seeks to address the question of why and in what ways have lawyers been importing economic theories into a legal environment, and how has this shaped scholarly research, judicial and legislative work? Since the financial crisis, corporate or capital markets law has been the focus of attention by academia and media. Formal modelling has been used to describe how capital markets work and, later, has been criticized for its abstract assumptions. Empirical legal studies and regulatory impact assessments offered different ways forward. This excellent book presents a new approach to the risks and benefits of interdisciplinary policy work. The benefits economic theory brings for reliable and tested lawmaking are contrasted with important challenges including the significant differences of research methodology, leading to misunderstandings and problems of efficient implementation of economic theory’s findings into the legal world. Katja Langenbucher’s innovative research scrutinizes the potential of economic theory to European legislators faced with a lack of democratic accountability.
Aristotle and Eudoxus on the Argument from Contraries
Authors: Cheng Wei 
Published: September 2020

Abstract
The debate over the value of pleasure among Eudoxus, Speusippus, and Aristotle is dramatically documented by the Nicomachean Ethics, particularly in the dialectical pros-and-cons concerning the so-called argument from contraries. Two similar versions of this argument are preserved at EN VII. 13, 1153b1–4, and X. 2, 1172b18–20. Many scholars believe that the argument at EN VII is either a report or an appropriation of the Eudoxean argument in EN X. This essay aims to revise this received view. It will explain why these two arguments differ in premises, contents and purposes, and why these distinctions matter for a proper assessment of Aristotle’s understanding of pleasure and pain in general and his dialectical art in particular.
The Battle of

psychê

and

thymos: A Reappraisal of Heraclitus’ Psychology
Authors: Mason Andrew J. 
Published: September 2020

Abstract
Heraclitus is generally recognised as the first of the Greek thinkers to develop a psychology, but the understanding of his psychology is held back by the assumptions that his soul is a life-principle and is ‘comprehensive’ of the various faculties we regard as psychological. The fragment that best displays the revolutionary character of Heraclitus’ soul doctrine, from a properly psychological viewpoint, is B 85. I offer an extended analysis of this fragment in order to bear out the claims, firstly, that psyche or ‘soul’ for Heraclitus is the intelligence alone, divine in origin, and secondly that thumos (primarily as the seat of desire) is a better candidate for his biological life-principle, whose exigencies are at odds with those of soul. I also offer some reflections on why, despite Heraclitus’ multifaceted influence on fifth-century thought, and despite the coherence of his psychology, this ‘revolution’ never quite took hold.