bluuebunny/crossref_metadata_2025_split huggingface dataset:
The benthic foraminifer Stomatorbina binkhorsti (Reuss, 1862): Taxonomic review and ecological insights
Authors: Schlagintweit Felix , Rigaud Sylvain 
Published: February 2020

Abstract
The benthic foraminifer Rosalina binkhorsti Reuss, 1862, was cosmopolitan in Late Cretaceous to early Paleogene shallow-water seas. It possesses a distinctive composite wall made of a continuous, agglutinated layer discontinuously covered by secondary hyaline outer deposits. Its taxonomic position, phylogeny, morphology, wall structure, and composition have been debated for a long time. Based on abundant, well-preserved material from the Danian of the Kambühel Formation in the Northern Calcareous Alps, Austria, we identify elements in the here emended species Stomatorbina binkhorsti which support a strong affinity to the order Textulariida, within the genus Stomatorbina Dorreen, 1948. Usually regarded as free (non-fixing), S. binkhorsti is here illustrated attached to small bioclasts in high-energy carbonate settings. The attached specimens are juvenile forms with a wall covered by massive hyaline deposits. This observation suggests that secondary lamellar parts added to the wall may have served for stabilisation or fixation to the substrate.

Rosalina binkhorsti Reuss, 1862, war eine in den Flachwassermeeren der Oberkreide und des frühen Paläogens kosmopolitische benthonische Foraminifere. Sie besitzt eine zusammengesetzte Wand, bestehend aus einer kontinuierlichen agglutinierten Lage welche diskontinuierlich von äusseren sekundär-hyalinen Abschnitten bedeckt ist. Ihre taxonomische Position, Phylogenie, Morphologie, Wandstruktur und –zusammensetzung ist seit langem umstritten. Basierend auf gut erhaltenem und reichhaltigem Material aus dem Danium der Kambühel Formation in den Nördlichen Kalkalpen von Österreich, werden Charakteristika identifiziert, welche nachhaltig eine Affinität zur Gattung Stomatorbina Dorreen, 1948 innerhalb der Ordnung Textulariida belegen. Diese Foraminiferenart, gewöhnlich als frei lebend (nicht fixiert) angesehen, wird illustriert fixiert an kleine Bioklasten in einem hochenergetischen karbonatischen Ablagerungsmilieu. Die fixierten Formen sind gewöhnlich juvenile Exemplare deren Wand mehr oder weniger massiv von hyalinen Ablagerungen bedeckt ist. Dies lässt vermuten, dass sekundäre lamellare Ablagerung, die der Wand hinzugefügt werden, eine Rolle in der Stabilisierung oder der Fixierung auf dem Substrat spielten.
Estudio de cohorte sobre el impacto del diagnóstico de displasia cervical e infección por Virus del Papiloma Humano en la función sexual de mujeres en un hospital de segundo nivel en Bogotá, Colombia
Authors: Celis-Amórtegui Marcela , Aldas-Gracia Jorge , Toro-Moreno Félix Andrés 
Published: February 2020

Introducción y objetivo: el Virus del Papiloma Humano (VPH) ha cobrado importancia gracias al advenimiento de técnicas de detección molecular que permiten su identificación antes de causar lesión en el epitelio cervical. El hecho de que esta enfermedad sea factor necesario para desarrollar cáncer de cérvix hace que ser portadora del VPH tenga además la connotación de verse expuesta al riesgo de cáncer. El objetivo de este estudio es determinar el impacto que tiene el diagnóstico de VPH, con o sin displasia, en la función sexual de las mujeres.Materiales y métodos: estudio de cohorte en el que se evaluó la función sexual femenina mediante el Cuestionario de Cambios (CSFQ-F®, por sus siglas en inglés), se incluyeron 134 mujeres, 71 con VPH y 63 sin VPH. La encuesta se implementó al ingreso al estudio y 6 semanas después. Se realizó análisis descriptivo de variables, bivariado y multivariado para las variables de interés.Resultados: el riesgo relativo de disfunción sexual en las mujeres con VPH y/o displasia cervical es de 4,4 (IC95% 1,617; 11,972). La disfunción se documentó desde la primera encuesta. No se encontraron diferencias significativas en la función sexual entre la primera y segunda encuesta. La t de Student pareada para cambio entre la primera y segunda encuesta no encontró diferencias significativas.Conclusiones: la infección por VPH se identifica en la Función Sexual como factor de riesgo para disfunción sexual femenina pero se necesitan estudios complementarios para confirmar esta asociación.
Síndrome de transfusión intergemelar estadio III, manejado con fotocoagulaición láser guiada por fetoscopia, complicado con Síndrome de Ballantyne y RCIU selectivo del feto donante: reporte de caso y revisión de la literatura
Authors: Gómez-Castro Libardo A. , Parra Guido , Vergara Felipe , Parra Miguel , Gómez Martha , Cuellar Rey , Quintero Rubén 
Published: February 2020

Objetivo: se presenta el caso de una paciente primigestante con embarazo monocorial biamniótico y síndrome de transfusión intergemelar estadio III, diagnosticado en la semana 23 de gestación. El manejo se llevó a cabo con foto-coagulación láser selectiva y secuencial de vasos comunicantes. Se presentó complicación con síndrome de Ballantyne y RCIU selectivo del feto donante. Desembarazada en la semana 35 con buen resultado perinatal. El objetivo es realizar una revisión actualizada de la literatura del síndrome de Ballantyne.Materiales y métodos: se revisaron las bases de datos PubMed, Cochrane, MEDLINE y libros de la especialidad, relativos al término “síndrome de Ballantyne”, sin restricción en la fecha de publicación.Conclusión: la fotocoagulación láser selectiva y secuencial de vasos comunicantes es el manejo de elección para el síndrome de transfusión fetofetal. La restricción de crecimiento selectivo del feto donante en estadio III se puede presentar en el 40 a 70% de los casos. El síndrome de Ballantyne puede ser secundario a edema placentario sin hidrops fetal; su sospecha puede ayudar a identificar cuáles pacientes se podrían beneficiar de un manejo activo de esta patología.
Astringency subqualities and sensory perception of Tuscan Sangiovese wines
Authors: Rinaldi Alessandra , Moine Virginie , Moio Luigi 
Published: February 2020

Aim: The Sangiovese grape cultivar is at the basis of the most well-known Italian wines produced in the Tuscany region. However, little is known about the sensory characteristics of Tuscan Sangiovese wines, and the diversity in astringency subqualities has never been investigated. In this study we evaluated the sensory perception of 16 commercial Sangiovese wines belonging to four categories of denomination (Chianti DOCG, CH; Chianti Classico DOCG, CC; Morellino di Scansano DOCG, MS; Toscana IGT, TS), and again after 20 months of bottle aging.Methods and results: A sensory evaluation was made, consisting of the astringency subqualities, taste, odor, and aroma profiles of wines. In addition, chemical analyses were carried out for the base parameters, polyphenols and some volatile compounds. Astringency subqualities varied depending on the percentage of Sangiovese in wines (from 80 % to 100 %). Blended Sangiovese wines were characterized by positive mouthfeel sensations. According to these, the drivers of liking the wines were associated with soft, mouthcoat, and rich subqualities. The Tuscan Sangiovese denominations were differentiated by volatile active compounds, whereas after about two years of bottle aging the astringency subqualities better achieved this task. Moreover, aging also influenced the evolution of wines: CC and CH wines positively evolved, revealing a complex odor profile; MS lost the fruity character; and TS was less involved in sensory modification.
Conclusions: For the first time, a detailed evaluation of the astringency subqualities of commercial Sangiovese wines was undertaken. Sangiovese subqualities differed according to the percentage of Sangiovese and denomination. Tuscan denominations were distinguished by peculiar sensory characteristics. In addition, bottle aging significantly influenced the evolution of the sensory perception of Sangiovese wine. In particular, mono-varietal Sangiovese wine needed a long period of aging to soften the astringency characteristics.Significance and impact of the study: Sangiovese wine represents - with its denominations and styles - the best-known and high-quality Italian wine in the world, and its popularity is increasing worldwide. Tuscan Sangiovese wines are often a blend with other red varieties of the region, and these can vary according to the production regulations. This study aimed to improve the knowledge of the sensory characteristics of Sangiovese wines belonging to different denominations and made with different percentages of this grape cultivar. In particular, the astringency subqualities are fundamental to fully appreciating the quality of the red wine during tasting.
Neurovascular Bundle Entrapment and Through Knee Amputation After Cerclage Cabling of Distal Femoral Peri-implant Fracture
Authors: Panagopoulos Andreas , Lachanas Ioannis , Kouzelis Antonis , Kokkalis Zinon T. , Tyllianakis Minos 
Published: February 2020

Case:
We report the case of an 82-year-old woman with diabetes, arteriosclerosis, chronic heart failure, and hypertension treated with an anatomical locking plate and multiple cables for a spiral-wedged periprosthetic fracture of the distal femur which was complicated by direct occlusion of the femoral artery and crush of the sciatic nerve, resulting in leg necrosis and, ultimately, through-knee disarticulation despite early recognition and arterial repair.

Conclusions:
Neurovascular injury is a potential complication of cerclage cables placement around the femoral shaft during complex fracture fixation. Devastating complications cannot always be corrected despite early vascular intervention.
Medial Epicondyle Fracture With Concomitant Flexor-Pronator Mass Avulsion From the Fracture Fragment
Authors: Mitchelson Andrew J. , Gabriel Keith R. 
Published: February 2020

Case:
We present a case of a pediatric patient who sustained a medial humeral epicondyle fracture with avulsion of the ulnar collateral ligament and flexor-pronator mass from the ossific nucleus fracture fragment. Treatment included excision of the medial epicondyle ossific nucleus and repair of the soft tissues. At 1-year, the patient had no pain, no elbow instability, and full and symmetric elbow range of motion.

Conclusion:
Excellent short-term pain and function outcomes can be observed in the pediatric patient after medial epicondyle fragment excision when there is concomitant avulsion of the ulnar collateral and flexor-pronator origins from the fracture fragment.
Below-Knee Amputation Resulting from an Arterial Thrombosis from Tourniquet Use in a Patient with Undiagnosed Factor V Leiden
Authors: Hautala Gavin , Liu Boshen , Hamilton David , Aneja Arun , Moghadamian Eric 
Published: February 2020

Case:
A 38-year-old woman presented with previously undiagnosed factor V Leiden (FVL), who suffered a complete superficial femoral arterial thrombosis after tourniquet use during the surgical repair of one of her bilateral tibial plafond fractures. This patient's injury eventually resulted in a below-knee amputation.

Conclusion:
We recommend expanding hypercoagulable screening on patients with risk factors based on a detailed history and physical examination. We also recommend limiting or negating tourniquet use in patients with FVL or other hypercoagulable disorders.
Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94
Authors: El-Naggar Noura El-Ahmady , El-Shweihy Nancy M. 
Published: February 2020

Abstract

Background: Cholesterol oxidase biosensors have been used to determine the level of cholesterol in different serum and food samples. Due to a wide range of industrial and clinical applications of microbial cholesterol oxidase, isolation and identification of a new microbial source (s) of cholesterol oxidase are very important. Results: The local isolate Streptomyces sp. strain NEAE-94 is a promising source of cholesterol oxidase. It was identified based on cultural, morphological and physiological characteristics; in addition to the 16S rRNA sequence. The sequencing product had been deposited in the GenBank database under the accession number KC354803. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 in shake flasks was optimized using surface response methodology. The different process parameters were first screened using a Plackett-Burman design and the parameters with significant effects on the production of cholesterol oxidase were identified. Out of the fifteen factors screened, agitation speed, cholesterol and yeast extract concentrations had the most significant positive effects on the production of cholesterol oxidase. The optimal levels of these variables and the effects of their mutual interactions on cholesterol oxidase production were determined using Box-Behnken design. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 was 11.03, 27.31 U/mL after Plackett-Burman Design and Box-Behnken design; respectively, with a fold of increase of 6.06 times compared to the production before applying the Plackett-Burman design (4.51 U/mL). Conclusions: Maximum cholesterol oxidase activity is obtained at the following fermentation conditions: g/L (cholesterol 4, yeast extract 5, NaCl 0.5, K 2 HPO 4 1, FeSO 4 .7H 2 O 0.01, MgSO 4 .7H 2 O 0.5), pH 7, inoculum size 4 % (v/v), temperature 37°C, agitation speed 150 rpm, medium volume 50 mL and incubation time 5 days.
Identification of Molecular Markers Associated With Ovarian Cancer Prognosis Using Bioinformatics Analysis
Authors: Chuang Li , Lyu Yuan , Liu Caixia 
Published: February 2020

Abstract
Background Ovarian cancer is associated with a high mortality rate worldwide. However, the pathogenesis, clinicopathological features, and genetic mechanisms of ovarian cancer are still unclear, and it is essential to identify prognostic markers for its clinical diagnosis and treatment. Here, we utilized bioinformatic analysis to identify potential genes involved in mediating BRCA1 expression to elucidate the potential mechanisms underlying ovarian cancer. Methods Gene expression profiling (GSE14407) was performed to identify differentially expressed genes (DEGs) and analyze the weighted gene co-expression network. We selected the key module that was significantly associated with BRCA1 expression and performed gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses for genes in the hub module. We then screened the hub genes utilizing the Search Tool for the Retrieval of Interacting Genes Database (STRING) and Molecular Complex Detection (MCODE) plug-in in Cytoscape. We validated gene expression levels through The Cancer Genome Atlas and GTEx databases for hub genes and screened genes that were related to overall survival in patients with ovarian cancer using the Kaplan-Meier plotter database. Results In total, 3124 DEGs were detected, including 433 upregulated genes and 2691 downregulated genes. We selected the brown module, which was the most significantly associated with BRCA1 expression. GO analysis showed that the hub module genes were significantly enriched in biological processes, including the mitotic cell cycle process, chromosome segregation, and cell division. KEGG analysis showed that the hub module genes were particularly enriched in the cell cycle, p53 signaling pathway, and small cell lung cancer. We selected 30 hub genes from the protein-protein interaction network, which had 88 nodes and 721 edges. Further analyses identified PBK as a prognosis-associated hub gene. Notably, PBK expression was significantly increased in ovarian cancer tissues, as demonstrated by immunohistochemistry analysis using samples from the Human Protein Atlas database.Conclusion PBK was found to be associated with overall survival in patients with ovarian cancer. Our results provide insights into our understanding of the molecular mechanisms and molecular diagnosis of ovarian cancer.
The Effect of Male Circumcision on Risky Sexual Behaviours of Men in Rwanda: A Comparative Analysis of RDHS 2005 and 2014/15
Authors: Nambawarr Chantine Pegha , Ntaganira Joseph 
Published: February 2020

Abstract
Background Rapid up-take of voluntary medical male circumcision (VMMC) in countries like Rwanda that implemented it as a national HIV prevention strategy since 2008, raised a public health concern of risk compensation. Risk compensation may occur if circumcised men increase their risky sexual behaviours (RSB) because of the awareness of circumcision’s 60% protection from heterosexual HIV/STIs transmission. The aim of this study was to assess the difference in RSBs among adolescent and adult circumcised men before and after the VMMC program implementation in Rwanda Methods Data of 11,037 men aged 15-59 years from Rwanda DHS 2005 and 2014/15 were analyzed using STATA version 13.0. We estimated the prevalence of ever paid sex , extramarital sex , more than 3 total lifetime sex partners, condom use at paid sex, first sex below age 15 and most recent sex partner being casual/commercial; among circumcised and uncircumcised men and compared between surveys. Association of these behaviours with circumcision status were also determined using bivariate and multivariate logistic regression analysis in separate and in combined datasets. Results Men who reported being circumcised almost tripled from 11%(532) in 2005 to 29%(1821) in 2014/15. In all men, RSB prevalence was lower in 2014/15 than in 2005. Circumcised men were generally more likely to engage in RSB in both surveys than uncircumcised men but less likely in 2014/15 compared to 2005. Multivariate models revealed no association of circumcision with sex below 15 years in 2005 (AOR 0.89; CI: 0.65-1.19; P>0.05) and 63% protection in 2014/15 (AOR 0.63; CI: 0.51-0.76; P<0.01). In all men, while there were too few responses to determine a true difference in non-condom use , other RSB were significantly less likely whereas paid sex was significantly more likely in 2014/15 due to an increase among the uncircumcised (AOR 1.29; CI: 1.03-1.61). Conclusion The findings did not support risk compensation. However, more responses on condom use are needed to check its disinhibition among circumcised men. Ongoing VMMC campaigns should re-inforce pre-counseling to young men 15-24 in the provinces out of Kigali to delay sexual debut even after circumcision.
Higher activity of monodehydroascorbate reductase and lower activities of leaf and spike vacuolar invertase and glutathione S-transferase reveals higher number of grains per spike in spring wheat genotypes grown under well-watered and drought conditions
Authors: Shokat sajid , Großkinsky Dominik K. , Roitsch Thomas , Liu Fulai 
Published: February 2020

Abstract
Background: To improve our understanding about the physiological mechanism of grain yield reduction at anthesis, three spring wheat genotypes (L1, L2 and L3) having contrasting yield potential under drought in field were investigated under controlled greenhouse conditions., drought stress was imposed at anthesis stage by withholding irrigation until all plant available water was depleted, while well-watered control plants were kept at 95% pot water holding capacity. Results: Compared to genotype L1 and L2, pronounced decrease in grain number (NGS), grain yield (GY) and harvest index (HI) were found in genotype L3, mainly due to its greater kernel abortion (KA) under drought. A significant positive correlation of leaf monodehydroascorbate reductase (MDHAR) with both NGS and HI was observed. In contrast, significant negative correlations of glutathione S-transferase (GST) and vacuolar invertase (vacInv) both within source and sink with NGS and HI were found. Likewise, a significant negative correlation of leaf abscisic acid (ABA) with NGS was noticed. Moreover, leaf aldolase and cell wall peroxidase (cwPOX) activities were significantly and positively associated with TKW. Conclusion: Collectively, distinct physiological markers were correlating with yield traits and higher activity of leaf aldolase and cwPOX may be chosen as predictive bio-markers for higher TKW. Also, higher activity of MDHAR within the leaf can be selected as a predictive bio-marker for higher NGS in wheat under drought. Whereas, lower activity of vacInv and GST both within leaf and spike can be selected as bio-markers for higher NGS and HI. The results highlighted the role of antioxidant and carbohydrate-metabolic enzymes in the modulation of source-sink balance in wheat crops, which could be used as bio-signatures for breeding and selection of drought-resilient wheat genotypes for a future drier climate.
A Retrospective Analysis: Development and Validation of a Nomogram Model for Predicating 30-day Mortality in ST-Segment Elevation Myocardial Infarction Patients
Authors: Wang Bin , Wang MaoFeng , Ying ManZhen , Liu Chengjiang 
Published: February 2020

Abstract
Objective: To develop and validate a personalized nomogram model to predict 30-day mortality in patients with ST-Segment Elevation Myocardial Infarction (STEMI).Methods: A total of 1144 STEMI patients admitted to Dongyang People’s Hospital from June 2013 to October 2019 were enrolled in the study and divided into two groups: Model group (n=801) and validation group (n=343). The biomarkers (maximum) in 24 hours after admission and the basic information of the patients were taken as variables, including creatinine, pro B-type natriuretic peptide (proBNP) and troponin T, as well as age, gender, etc. Logistic stepwise regression was employed to screen out independent risk factors that may led to death in patients with acute STEMI. A nomogram predication model was established with the screened independent risk factors as variables. Area underReceiver Operating Characteristic (ROC) curve and GiViTI calibration chart were adopted to estimate the classification accuracy and calibrate the predication model, respectively, and decline curve analysis (DCA) to assess the clinical efficiency of the model.Results: Logistic stepwise regression revealed that proBNP, creatine kinase-MB (CK-MB), age, creatinine, lactic acid and percutaneous coronary intervention (PCI) surgery or not were independent risk factors that decided whether a STEMI patient would die. After the establishment of the model, area under ROC curve was 0.897 and 0.859 in model group and validation group, respectively, suggesting that the new model was a good classifier. P value of GiViTI calibration test was 0.834 and 0.262, respectively. P>0.05 in GiViTI calibration indicated a high goodness of fit between the predication ratio of the model and the actual ratio, showing that the calibration of predications models in model group and validation group was complete. DCA curves in model group and validation group were both above two extreme curves, which suggested a good clinical significance.Conclusion: The personalized model for predicating 30-day mortality in patients with STEMI will help screen out those STEMI patients who may die in a short term.