bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary conization overcomes the risk of developing local recurrence following laparoscopic radical hysterectomy in early stage cervical cancer
Authors: Bogani Giorgio , Ditto Antonino , Chiappa Valentina , Pinelli Ciro , Sonetto Cristina , Raspagliesi Francesco 
Published: June 2020

AbstractObjectiveTo investigate whether primary conization might overcome the risk of local dissemination in patients undergoing laparoscopic radical hysterectomy.MethodsConsecutive data of 262 patients with early stage cervical cancer were retrieved: 88 women had conization followed by radical hysterectomy. A propensity‐matched comparison (1:1) was carried out in order to compare laparoscopy and open surgery. Accumulating data highlighted that minimally invasive surgery has been associated with higher recurrence rates and worse overall survival than open surgery in women with early stage cervical cancer.ResultsData of 35 paired patients (total 70 patients) were analyzed. No between‐group differences in baseline, disease, and pathological variables were observed. Patients undergoing laparoscopy correlated with lower blood loss (50 [range 30–100] vs 150 [range 50–500] mL; P<0.001) and shorter length of stay (3 ± 0.8 vs 5.4 ± 1.4 days; P<0.001) compared to open surgery. One local recurrence was observed per group (P=1.00). Type of surgical approach did not influence site of recurrence (P=1.00) or survival outcomes, in terms of 10‐year disease‐free (P=0.549, log‐rank test) and overall survivals (P=0.615, log‐rank test).ConclusionsThe data show that primary conization might overcome the risk of local recurrence after laparoscopic radical hysterectomy in early stage cervical cancer. Further prospective evidence is needed.
Malaria in the third trimester and maternal‐perinatal outcome
Authors: Nkwabong Elie , Mayane Diane N. , Meka Esther , Essiben Felix 
Published: June 2020

AbstractObjectiveTo evaluate maternal and perinatal outcomes in cases of third‐trimester malaria (TTM).MethodsA parity‐matched comparative cohort study was carried out between December 1, 2018, and April 30, 2019, in three university teaching hospitals in Yaoundé (Cameroon). Women with and without TTM were followed up till delivery. The variables analyzed included maternal and gestational ages at delivery, the regimen of intermittent preventive treatment, usage of insecticide‐treated net, history of malaria recorded during pregnancy, birth and placenta weights, Apgar score, and early neonatal outcomes. Fisher exact test, t‐test, and logistic regression were used for comparison. P<0.05 was considered statistically significant.ResultsOf 3063 pregnant women, 130 (4.2%) had TTM. Adverse outcomes associated with TTM were maternal anemia (relative risk [RR] 10, 95% confidence interval [CI] 4.91–20.34), intrauterine fetal demise (RR 7.50, 95% CI 1.47–38.06), preterm delivery (RR 4.50, 95% CI 2.37–8.51), low birth weight (adjusted RR 2.88, 95% CI 1.34–6.19), neonatal asphyxia especially if delivery occurred during parenteral treatment (RR 5.18, 95% CI 2.56–10.48), transfer of the newborn to the neonatal intensive care unit (RR 4.38, 95% CI 2.59–7.42), and intrapartum or early neonatal death (RR 4.18, 95% CI 1.48–11.74).ConclusionTTM was associated with adverse perinatal outcome especially if labor started during parenteral treatment.
Gene-Metabolite Network Linked to Inhibited Bioenergetics in Association With Spaceflight-Induced Loss of Male Mouse Quadriceps Muscle
Authors: Chakraborty Nabarun , Waning David L , Gautam Aarti , Hoke Allison , Sowe Bintu , Youssef Dana , Butler Stephan , Savaglio Michael , Childress Paul J , Kumar Raina , Moyler Candace , Dimitrov George , Kacena Melissa A , Hammamieh Rasha 
Published: June 2020

ABSTRACT
Prolonged residence of mice in spaceflight is a scientifically robust and ethically ratified model of muscle atrophy caused by continued unloading. Under the Rodent Research Program of the National Aeronautics and Space Administration (NASA), we assayed the large-scale mRNA and metabolomic perturbations in the quadriceps of C57BL/6j male mice that lived in spaceflight (FLT) or on the ground (control or CTR) for approximately 4 weeks. The wet weights of the quadriceps were significantly reduced in FLT mice. Next-generation sequencing and untargeted mass spectroscopic assays interrogated the gene-metabolite landscape of the quadriceps. A majority of top-ranked differentially suppressed genes in FLT encoded proteins from the myosin or troponin families, suggesting sarcomere alterations in space. Significantly enriched gene-metabolite networks were found linked to sarcomeric integrity, immune fitness, and oxidative stress response; all inhibited in space as per in silico prediction. A significant loss of mitochondrial DNA copy numbers in FLT mice underlined the energy deprivation associated with spaceflight-induced stress. This hypothesis was reinforced by the transcriptomic sequencing–metabolomics integrative analysis that showed inhibited networks related to protein, lipid, and carbohydrate metabolism, and adenosine triphosphate (ATP) synthesis and hydrolysis. Finally, we discovered important upstream regulators, which could be targeted for next-generation therapeutic intervention for chronic disuse of the musculoskeletal system. © 2020 The Authors. Journal of Bone and Mineral Research published by American Society for Bone and Mineral Research.
Long‐term consequences of the 2020 coronavirus pandemics: Historical global‐macro context
Authors: Tokic Damir 
Published: June 2020

AbstractThe COVID‐19 pandemics is likely to accelerate the trends of de‐globalization and de‐dollarization. Over the long term, the major financial consequence is likely to be the accelerated trend of rising inflation and long‐term interest rates. More broadly, the post‐pandemic environment will likely encompass difficult longer‐term economic and geopolitical challenges. Alternatively, the COVID‐19 pandemics also creates an opportunity for building a new trend of more sustainable globalization.
Searching new structures of beryllium‐doped in small‐sized magnesium clusters: Be2MgnQ (Q = 0, −1; n = 1–11) clusters DFT study
Authors: Zeng Lu , Deng Ping‐Ji , Bi Jie , Zhu Ben‐Chao 
Published: June 2020

AbstractUsing CALYPSO method to search new structures of neutral and anionic beryllium‐doped magnesium clusters followed by density functional theory (DFT) calculations, an extensive study of the structures, electronic and spectral properties of Be2MgnQ (Q = 0, −1; n = 2–11) clusters is performed. Based on the structural optimization, it is found that the Be2MgnQ (Q = 0, −1) clusters are shown by tetrahedral‐based geometries at n = 2–6 and tower‐like‐based geometries at n = 7–11. The calculations of stability indicate that Be2Mg5Q=0, Be2Mg5Q=−1, and Be2Mg8Q=−1 clusters are “magic” clusters with high stability. The NCP shows that the charges are transferred from Mg atoms to Be atoms. The s‐ and p‐orbitals interactions of Mg and Be atoms are main responsible for their NEC. In particular, chemical bond analysis including molecular orbitals (MOs) and chemical bonding composition for magic clusters to further study their stability. The results confirmed that the high stability of these clusters is due to the interactions between the Be atom and the Mg5 or Mg8 host. Finally, theoretical calculations of infrared and Raman spectra of the ground state of Be2MgnQ (Q = 0, −1; n = 1–11) clusters were performed, which will be absolutely useful for future experiments to identify these clusters.
Single‐cell RNA analysis on ACE2 expression provides insights into SARS‐CoV‐2 potential entry into the bloodstream and heart injury
Authors: Guo Jieyu , Wei Xiangxiang , Li Qinhan , Li Liliang , Yang Zhaohua , Shi Yu , Qin Yue , Zhang Xinyue , Wang Xinhong , Zhi Xiuling , Meng Dan 
Published: June 2020

AbstractCoronavirus disease‐2019 (COVID‐19) is a global pandemic with high infectivity and pathogenicity, accounting for tens of thousands of deaths worldwide. Recent studies have found that the pathogen of COVID‐19, severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), shares the same cell receptor angiotensin converting enzyme II (ACE2) as SARS‐CoV. The pathological investigation of COVID‐19 deaths showed that the lungs had characteristics of pulmonary fibrosis. However, how SARS‐CoV‐2 spreads from the lungs to other organs has not yet been determined. Here, we performed an unbiased evaluation of cell‐type‐specific expression of ACE2 in healthy and fibrotic lungs, as well as in normal and failed adult human hearts, using published single‐cell RNA‐seq data. We found that ACE2 expression in fibrotic lungs mainly locates in arterial vascular cells, which might provide a route for bloodstream spreading of SARS‐CoV‐2. Failed human hearts have a higher percentage of ACE2‐expressing cardiomyocytes, and SARS‐CoV‐2 might attack cardiomyocytes through the bloodstream in patients with heart failure. Moreover, ACE2 was highly expressed in cells infected by respiratory syncytial virus or Middle East respiratory syndrome coronavirus and in mice treated by lipopolysaccharide. Our findings indicate that patients with pulmonary fibrosis, heart failure, and virus infection have a higher risk and are more susceptible to SARS‐CoV‐2 infection. The SARS‐CoV‐2 might attack other organs by getting into the bloodstream. This study provides new insights into SARS‐CoV‐2 blood entry and heart injury and might propose a therapeutic strategy to prevent patients from developing severe complications.
JunB can enhance the transcription of IL‐8 in oral squamous cell carcinoma
Authors: Tsunoda Mariko , Fukasawa Mai , Nishihara Anna , Takada Leo , Asano Masatake 
Published: June 2020

AbstractProteasome inhibitor MG132 was shown to enhance the secretion of interleukin 8 (IL‐8) by various cells. The enhancement is regulated by the transcription factor activator protein‐1 (AP‐1) at the transcriptional level. AP‐1 is a dimer formed by AP‐1 family proteins. The purpose of the present study was to explore the combinations of the AP‐1 family proteins that contribute to MG132‐driven IL‐8 secretion. Oral squamous cell carcinoma‐derived cell lines, Ca9‐22 and HSC3, were used to demonstrate their response to MG132. IL‐8 secretion was augmented by MG132 in both cell lines. c‐Jun expression was detected in both the cell lines, whereas c‐Fos expression was detected only in the HSC3. The influence of MG132 stimulation on c‐Jun and c‐Fos expression was further examined by western blot analysis. c‐Jun expression was increased by MG132 stimulation, whereas c‐Fos expression was not detected even after MG132 stimulation. As JunB is reported to inhibit the transcriptional activity of the AP‐1 complex, we speculated that the c‐Jun homodimer should contribute to IL‐8 enhancement. Expression vectors encoding wild type and c‐Jun mutants, M17 and M22‐23, respectively, were constructed and transfected into the Ca9‐22 cells. In contrast to our expectations, MG132‐induced IL‐8 secretion was significantly reduced in all the transfectants suggesting that other c‐Jun members might form homodimers with c‐Jun and contribute to IL‐8 enhancement. Transfection of the cells with c‐Jun or JunB small hairpin RNA (shRNA) reduced IL‐8 secretion up to 50% and 65% of the control shRNA transfectant. Furthermore, cotransfection of both shRNA almost completely inhibited the IL‐8 secretion. These results indicate that JunB not only inhibits but also enhances the transcription of c‐Jun targets in combination with c‐Jun.
Low shear stress regulates vascular endothelial cell pyroptosis through miR‐181b‐5p/STAT‐3 axis
Authors: Xu Xiangshan , Yang Yang , Wang Guofeng , Yin Yu , Han Shuo , Zheng Donghan , Zhou Shaobo , Zhao Yuanyuan , Chen Yong , Jin Yuanzhe 
Published: June 2020

AbstractLow shear stress and pyroptosis both play an important role in the onset and development of atherosclerosis (AS). MicroRNAs (miRNAs) are a kind of short (18–22) nucleotide sequences that can bind to the 3′‐untranslated region (3′‐UTR) of messenger RNA, thereby regulating programmed cell death including pyroptosis. However, the function of miRNAs in cells subjected to shear stress conditions is unknown. Therefore, we conducted the current study to demonstrate the effect of low shear stress on pyroptosis and the underlying mechanism. Human umbilical vein endothelial cells (HUVECs) stimulated by undisturbed shear stress (5 dynes/cm2) were the experimental group while HUVECs without shear stress treatment were the control group in our experiments. We observed that shear stress can suppress mechanosensitive miR‐181b‐5p expression, accompanying the elevated expression of NLRP3 inflammasome‐dependent pyroptosis. Introduction of miR‐181b‐5p could alleviate NLRP3 inflammasome‐dependent pyroptosis. Luciferase assay showed specific binding of miR‐181b‐5p to the 3′‐UTR of signal transduction and transcriptional activation factor 3 (STAT‐3) gene. Inhibition of STAT‐3 gene expression at the posttranscriptional level results in the alleviation of NLRP3 inflammasome‐dependent pyroptosis. Besides, the silencing of STAT‐3 reduced anti‐miR‐181b‐5p‐mediated HUVEC pyroptosis via regulating NLRP3 inflammasome activation. Given the role of mechanosensitive miR‐181b‐5p and STAT‐3 in the shear stress‐induced pyroptosis, regulation of their expression levels may be a promising strategy to control AS.
What are the consequences of Methylphenidate exposure for maternally separated rats?
Authors: Mohtashami Borzadaran Fatemeh , Esmaeilpour Khadijeh , Joushi Sara , Rajizadeh Mohammad Amin , Sheibani Vahid , ur Rehman Naeem , Sepehri Gholamreza 
Published: June 2020

AbstractMethylphenidate (MPH) abuse is prevalent among youth. Drug abuse results in pain perception. We sought to investigate whether Maternal separation (MS) prone to MPH addiction. The next question was whether MPH abusers with MS differ in pain perception. We investigated the impact of MS on addiction and drug reward as well as pain perception following 5 days of MPH injection in males and females rats. Initially, rats underwent MS protocol of 3 hr daily for 21 days. Conditioned place preference (CPP) test was an attempt to investigate whether MS rats experience more reward with MPH. The protocol consisted of 10 min habituation on Day 1, conditioning on Day 2–Day 6 (5 mg per kg MPH injection in drug compartment and saline in saline compartment with 4 hr gap between injections) and 10 min test on Day 7. Furthermore, using another group, differences in pain perception were investigated after 5 days of daily MPH injection with 5 mg per kg. Sensory pain sensitivity was tested on PND 39 using tail flick and hotplate in MS and control groups with and without MPH exposure. Results indicated that female rats are equally prone to addiction in CPP. On the other hand, MS males experience a higher reward in CPP. In tail flick test, female MS rats exposed to MPH show a lower sensory pain threshold with similar MPH exposure. Experiencing MPH similarly declined hotplate pain perception in MS and controls in the females. Males, on the other hand, did not show any difference in sensory pain tests. According to results one can argue MS is detrimental. MS males experience more reward with MPH, females are equally addiction prone and MS females experience more pain in tail flick. On the other hand pain threshold can decline in hotplate test for both control and MS females that received MPH.
Clinicopathological characteristics of intraductal papillary neoplasm of the bile duct: a Japan‐Korea collaborative study
Authors: Kubota Keiichi , Jang Jin‐Young , Nakanuma Yasuni , Jang Kee‐Taek , Haruyama Yasuo , Fukushima Noriyoshi , Furukawa Toru , Hong Seung‐Mo , Sakuraoka Yuhki , Kim Haeryoung , Matsumoto Takatsugu , Lee Kyung Bun , Zen Yoh , Kim Jaeri , Miyazaki Masaru , Choi Dong Wook , Heo Jin Seok , Endo Itaru , Hwang Shin , Nakamura Masafumi , Han Ho‐Seong , Uemoto Shinji , Park Sang Jae , Hong Eun Kyung , Nanashima Atsushi , Kim Dong‐Sik , Kim Joo Young , Ohta Tetsuo , Kang Koo Jeong , Fukumoto Takumi , Nah Yang Won , Seo Hyung Il , Inui Kazuo , Yoon Dong‐Sup , Unno Michiaki 
Published: June 2020

AbstractBackgroundThe prevalent location and incidence of intraductal papillary neoplasm of the bile duct (IPNB) and invasive carcinoma associated with them have varied markedly among studies due to differences in diagnostic criteria and tumor location.MethodsIPNBs were classified into two types: Type 1 IPNB, being histologically similar to intraductal papillary mucinous neoplasm of the pancreas, and Type 2 IPNB, having a more complex histological architecture with irregular papillary branching or foci of solid‐tubular components. Medical data were evaluated.ResultsAmong 694 IPNB patients, 520 and 174 had Type 1 and Type 2, respectively. The levels of AST, ALT, ALP, T. Bil, and CEA were significantly higher in patients with Type 2 than in those with Type 1. Type 1 IPNB was more frequently located in the intrahepatic bile duct than Type 2, whereas Type 2 was more frequently located in the distal bile duct than Type 1 IPNB (P < 0.001). There were significant differences in 5‐year cumulative survival rates (75.2% vs 50.9%; P < 0.0001) and 5‐year cumulative disease‐free survival rates (64.1% vs 35.3%; P < 0.0001) between the two groups.ConclusionType 1 and Type 2 IPNBs differ in their clinicopathological features and prognosis. This classification may help to further understand IPNB.
Safety of a new spray‐type adhesion barrier (AdSpray®) in liver surgery
Authors: Okubo Satoshi , Shindoh Junichi , Kobayashi Yuta , Hashimoto Masaji 
Published: June 2020

AbstractBackgroundAlthough adhesion barriers are increasingly used in liver surgery, there are no sufficient data yet in support of their safety.MethodsData of 104 consecutive patients in whom a spray‐type adhesion barrier (AdSpray®) was used after hepatectomy were compared with those of 94 control subjects in whom no adhesion barriers were used, and the risk factors for major morbidity after hepatectomy were investigated.ResultsThe incidence of major morbidity was not significantly different between the AdSpray group and the control group (17% vs 15%, P = .645), although multivariate analyses revealed that the use of AdSpray was associated with an increased risk of abdominal abscess (odds ratio [OR], 4.42; P = .043). Similar tendency was also confirmed after propensity score adjustment to account for the potential selection bias. A sub‐group analysis revealed that incidence of abscess formation decreased as operators' experience in the used of AdSpray increased, and multivariate analysis identified preoperative chemotherapy (OR, 3.96; P = .043) as being independent predictors of abscess formation after the use of AdSpray.ConclusionsAdSpray use was not associated with global incidence of major morbidity after liver surgery, although surgeon's experience would be needed for adequate use of AdSpray to minimize the risk of postoperative abscess formation.
Trends in the use of implantable cardioverter‐defibrillator and cardiac resynchronization therapy device in advancing age: Analysis of the Japan cardiac device treatment registry database
Authors: Yokoshiki Hisashi , Shimizu Akihiko , Mitsuhashi Takeshi , Ishibashi Kohei , Kabutoya Tomoyuki , Yoshiga Yasuhiro , Kohno Ritsuko , Abe Haruhiko , Nogami Akihiko ,   Members of the Implantable Cardioverter-Defibrillator (ICD) Committee of the Japanese Heart Rhythm Society
Published: June 2020

AbstractBackgroundTrends of de novo implantation of cardiac implantable electronic devices (CIEDs) including implantable cardioverter‐defibrillator (ICD) and cardiac resynchronization therapy with a defibrillator (CRT‐D) or pacemaker (CRT‐P) in advancing age are unknown.MethodsAnalysis of data from the Japan cardiac device treatment registry (JCDTR) with an implantation date between January 2006 and December 2016 was performed focusing on advancing age of ≧75 years.ResultsThe cohort included 17 564 ICD, 9470 CRT‐D and 1087 CRT‐P recipients for de novo implantation. The rate of patients ≧75 years of age increased from 17.1% to 20.5% in ICD implantation (P = .052), from 19.7% to 30.0% in CRT‐D implantation (P < .0001), and from 40.0% to 64.0% in CRT‐P implantation (P = .17). There was an apparent increase in the percentage of nonischemic patients aged ≧75 years receiving ICD (10.9% in 2006 to 16.4% in 2016, P = .0008) and CRT‐D (17.1% in 2006 to 27.8% in 2016, P = .0001). The implantation for primary prevention ICD (P = .059) and CRT‐D (P = .012) was also associated with a temporal increase in the percentage of patients aged ≧75 years.ConclusionsProportion of patients ≧75 years of age for de novo CIED implantation gradually increased from 2006 to 2016, presumably because of the growing number of nonischemic cardiomyopathy and heart failure patients requiring primary prevention of sudden cardiac death.
Supplementation of Aspergillus glaucus with gfdB gene encoding a glycerol 3‐phosphate dehydrogenase in Aspergillus nidulans
Authors: Király Anita , Szabó Ivett G. , Emri Tamás , Leiter Éva , Pócsi István 
Published: June 2020

AbstractIn Aspergillus nidulans, there are two putative glycerol 3‐phosphate dehydrogenases encoded by the genes gfdA and gfdB, while the genome of the osmophilic Aspergillus glaucus harbors only the ortholog of the A. nidulans gfdA gene. Our aim was to insert the gfdB gene into the genome of A. glaucus, and we reached this goal with the adaptation of the Agrobacterium tumefaciens‐mediated transformation method. We tested the growth of the gfdB‐complemented A. glaucus strains on a medium containing 2 mol l‒1 sorbitol in the presence of oxidative stress generating agents such as tert‐butyl hydroperoxide, H2O2, menadione sodium bisulfite, as well as the cell wall integrity stress‐inducing agent Congo Red and the heavy metal stress eliciting CdCl2. The growth of the complemented strains was significantly higher than that of the wild‐type strain on media supplemented with these stress generating agents. The A. nidulans ΔgfdB mutant was also examined under the same conditions and resulted in a considerably lower growth than that of the control strain in all stress exposure experiments. Our results shed light on the fact that the gfdB gene from A. nidulans was also involved in the stress responses of the complemented A. glaucus strains supporting our hypothesis on the antioxidant function of GfdB in the Aspergilli. Nevertheless, the osmotolerant nature of A. glaucus could not be explained by the lack of the gfdB gene in A. glaucus, as we hypothesized earlier.
Influence of attractions on destination selection
Authors: McKercher Bob , Tolkach Denis 
Published: June 2020

AbstractThis study tests the juxtaposition of Pearce's Travel Career Pattern with McKercher's tourism product taxonomy by examining the relationship between motive and the role attractions' taxa play in influencing the decision to visit a destination. The study represents the third in a series of three papers, with the first two presenting a conceptual model and testing the model with secondary data, respectively. The study concluded that Pearce's core motives were associated with tourists seeking a destination experience, while when respondents expressed middle and outer layer motives, they tended to seek more of an attraction specific experience.