bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-molecule FRET monitors CLC transporter conformation and subunit independence
Authors: Cheng Ricky C. , Krishnamoorti Ayush , Berka Vladimir , Durham Ryan J , Jayaraman Vasanthi , Maduke Merritt 
Published: September 2020

Abstract“CLC” transporters catalyze the exchange of chloride ions for protons across cellular membranes. As secondary active transporters, CLCs must alternately allow ion access to and from the extracellular and intracellular sides of the membrane, adopting outward-facing and inward-facing conformational states. Here, we use single-molecule Förster resonance energy transfer (smFRET) to monitor the conformational state of CLC-ec1, anE. colihomolog for which high-resolution structures of occluded and outward-facing states are known. Since each subunit within the CLC homodimer contains its own transport pathways for chloride and protons, we developed a labeling strategy to follow conformational change within a subunit, without crosstalk from the second subunit of the dimer. Using this strategy, we evaluated smFRET efficiencies for labels positioned on the extracellular side of the protein, to monitor the status of the outer permeation pathway. When [H+] is increased to enrich the outward-facing state, the smFRET efficiencies for this pair decrease. In a triple-mutant CLC-ec1 that mimics the protonated state of the protein and is known to favor the outward-facing conformation, the lower smFRET efficiency is observed at both low and high [H+]. These results confirm that the smFRET assay is following the transition to the outward-facing state and demonstrate the feasibility of using smFRET to monitor the relatively small (~1 Å) motions involved in CLC transporter conformational change. Using the smFRET assay, we show that the conformation of the partner subunit does not influence the conformation of the subunit being monitored by smFRET, thus providing evidence for the independence of the two subunits in the transport process.SUMMARYCheng, Krishnamoorti et al. use single-molecule Förster energy resonance transfer measurements to monitor the conformation of a CLC transporter and to show that the conformational state is not influenced by the neighboring subunit.
The effect of self-efficacy on job performance through creativity: the moderating roles of customer incivility and service scripts
Authors: Hur Won-Moo , Moon Tae-Won , Lee Jun-Ho 
Published: September 2020

PurposeThis study aimed to examine the effect of self-efficacy on job performance through creativity. We predicted that exposure to customer incivility and rigid service scripts will moderate the mediating effect of creativity on the relationship between self-efficacy and job performance.Design/methodology/approachSurvey data were collected from 397 salespersons who were working in a department store in South Korea. The PROCESS macro was used to test the study hypotheses.FindingsThe results revealed that the positive relationship between self-efficacy and job performance was partially mediated by creativity. Furthermore, exposure to customer incivility and rigid service scripts weakened the positive effects of self-efficacy on creativity. Finally, customer incivility and rigid service scripts also dampened the positive effects of self-efficacy on job performance through creativity.Research limitations/implicationsSales organizations should understand that the extent to which self-efficacy improves job performance by enhancing creativity is contingent on the extent to which salespersons are exposed to customer incivility and are required to adhere to rigid service scripts in the workplace.Originality/valueOur findings paint a more complete portrait of the beneficial effects of self-efficacy. Specifically, they suggest that the development of creativity is an important mechanism that underlies the process by which internal resources enhance job performance and that customer incivility and service scripts weaken this relationship.
Something borrowed, something blue: reflections on theory borrowing in educational administration research
Authors: Berkovich Izhak 
Published: September 2020

PurposeThe practice of theory borrowing from other research fields is common in interdisciplinary and applied research. Nevertheless, educational administration researchers seldom discuss this phenomenon and its complexities in depth.Design/methodology/approachThis essay provides an overview of what has been written about the practice of theory borrowing.FindingsAfter presenting the criticism on misusing theory borrowing, it outlines several recommendations to improve theory borrowing in education administration research by domesticating it through conceptual blending.Originality/valueThe purpose of this essay is to motivate educational administration scholars to reflect on the practice of theory borrowing. The guidelines offered here for promoting conceptual blending serve as a middle ground for mitigating a key problem of theory borrowing.
Acoustic monitoring data of anuran species inside and outside the evacuation zone of the Fukushima Daiichi power plant accident
Authors: Yoshioka Akira , Matsushima Noe , Jingu Shoma , Kumada Nao , Yokota Ryoko , Totsu Kumiko , Fukasawa Keita 
Published: September 2020

AbstractThe Fukushima Daiichi power plant accident led to large‐scale and long‐term evacuation zones in which usual land‐use activities such as farming have been stopped. In particular, the loss of irrigated rice paddies is hypothesized to have seriously impacted freshwater biodiversity. In 2014, we started acoustic monitoring of frogs by using digital voice recorders in and around the evacuation zone. For the monitoring project, 52 and 57 monitoring sites were located within schoolyards (including those that had been converted into community centers) to examine the frog assemblages in the urban and rural landscapes of the region in 2014 and 2015, respectively. At each site, a digital voice recorder was installed during the period from May to July, and we recorded 10 min a day at night using a timed‐recording mode. We divided the audio data into 20‐s segments and identified species recorded in segments sampled from late May to late June (partly in early July). We identified eight frog species from 1,962 audio segments in total (correspond to 4 days per year in principal). For each species, intensity of calling at four levels was also recorded as an index of abundance. We are continuing to monitor and intend to update the dataset with new observations hereafter. Our dataset will support scientists and experts in recognizing the status and dynamics of anuran assemblages in and around the evacuation zone and will contribute to the promotion of open science.The complete data set for this abstract published in the Data Paper section of the journal is available in electronic format in MetaCat in JaLTER at http://db.cger.nies.go.jp/JaLTER/metacat/metacat/ERDP-2020-12.1/jalter-en.
Quantitative multiplex immunofluorescence analysis identifies infiltrating PD1+CD8+ and CD8+ T cells as predictive of response to neoadjuvant chemotherapy in breast cancer
Authors: Liang Hongling , Li Hongsheng , Xie Zhi , Jin Tianen , Chen Yu , Lv Zhiyi , Tan Xiaojun , Li Jia , Han Guodong , He Weixing , Qiu Ni , Jiang Ming , Zhou Jie , Xia Haoming , Zhan Yongtao , Cui Lulu , Guo Weiling , Huang Jianqing , Zhang Xuchao , Wu Yi‐long 
Published: September 2020

BackgroundThis study aimed to explore the potentially predictive role and dynamic changes of immune checkpoints on T cell subsets in patients with breast cancer receiving neoadjuvant chemotherapies.MethodsFluorescent multiplex immunohistochemistry (mIHC) was used to stain CD4, CD8, PD1, TIM3, and cytokeratins simultaneously in paired breast cancer samples before and after neoadjuvant therapies (NAT) in a prospective cohort (n = 50). Singleplex IHC was conducted to stain for CD3 in 100 cases with inclusion of extra retrospective 50 cases. Cell levels were correlated with clinicopathological parameters and pathological complete response (pCR).ResultsIn pretreatment tumors, the percentages of infiltrating CD8+, PD1+, PD1+CD8+, and the ratio of PD1+CD8+/CD8+ cells, were higher in pCR than non‐pCR patients in either the stromal or intratumoral area, but PD1+CD4+, TIM3+CD4+, TIM3+CD8+ cells and CD4+/CD8+ ratio was not. Multivariate analyses showed that the percentage of intratumoral CD8+ cells (OR, 1.712; 95% CI: 1.052–2.786; P = 0.030) and stromal PD1+CD8+/CD8+ ratio (OR, 1.109; 95% CI: 1.009–1.218; P = 0.032) were significantly associated with pCR. Dynamically, reduction in the percentages of PD1+, CD8+ and PD1+CD8+ cells after therapy strongly correlated with pCR. Notably, incremental percentages of PD1+CD8+ cells, rather than TIM3+CD8+, were shown in tumors from non‐pCR patients after NAT. CD3 staining confirmed the percentage of T cells were associated with pCR.ConclusionsPD1+CD8+ rather than TIM3+CD8+ cells are main predictive components within tumor‐infiltrating T cells in NAT breast cancer patients. Dynamically incremental levels of PD1+CD8+ cells occurred in non‐pCR cases after NAT, suggesting the combination of chemotherapy with PD1 inhibition might benefit these patients.Key pointsSignificant findings of the study
PD1+CD8+, rather than TIM3+CD8+, T cells are the main component to predict the response of neoadjuvant therapies in breast cancer.
What this study adds
Incremental levels of PD1+CD8+ T cells in non‐pCR post‐NAT tumors suggest PD1 inhibition might benefit in the neoadjuvant setting.
Targeting UDP‐glucose dehydrogenase inhibits ovarian cancer growth and metastasis
Authors: Lin Li‐Hsun , Chou Hsiu‐Chuan , Chang Shing‐Jyh , Liao En‐Chi , Tsai Yi‐Ting , Wei Yu‐Shan , Chen Hsin‐Yi , Lin Meng‐Wei , Wang Yi‐Shiuan , Chien Yu‐An , Yu Xin‐Ru , Chan Hong‐Lin 
Published: September 2020

AbstractMore than 70% of patients with ovarian cancer are diagnosed in advanced stages. Therefore, it is urgent to identify a promising prognostic marker and understand the mechanism of ovarian cancer metastasis development. By using proteomics approaches, we found that UDP‐glucose dehydrogenase (UGDH) was up‐regulated in highly metastatic ovarian cancer TOV21G cells, characterized by high invasiveness (TOV21GHI), in comparison to its parental control. Previous reports demonstrated that UGDH is involved in cell migration, but its specific role in cancer metastasis remains unclear. By performing immunohistochemical staining with tissue microarray, we found overexpression of UGDH in ovarian cancer tissue, but not in normal adjacent tissue. Silencing using RNA interference (RNAi) was utilized to knockdown UGDH, which resulted in a significant decrease in metastatic ability in transwell migration, transwell invasion and wound healing assays. The knockdown of UGDH caused cell cycle arrest in the G0/G1 phase and induced a massive decrease of tumour formation rate in vivo. Our data showed that UGDH‐depletion led to the down‐regulation of epithelial‐mesenchymal transition (EMT)‐related markers as well as MMP2, and inactivation of the ERK/MAPK pathway. In conclusion, we found that the up‐regulation of UGDH is related to ovarian cancer metastasis and the deficiency of UGDH leads to the decrease of cell migration, cell invasion, wound healing and cell proliferation ability. Our findings reveal that UGDH can serve as a prognostic marker and that the inhibition of UGDH is a promising strategy for ovarian cancer treatment.
Dose–Response Relationship Between External Load and Wellness in Elite Women’s Soccer Matches: Do Customized Velocity Thresholds Add Value?
Authors: Scott Dawn , Norris Dean , Lovell Ric 
Published: September 2020

Purpose: To examine the dose–response relationship between match-play high-speed running (HSR), very high-speed running (VHSR), and sprint (SPR) distances versus subsequent ratings of fatigue and soreness. Methods: Thirty-six outfield players competing in the professional National Women’s Soccer League (NWSL, United States) with a minimum of five 90-minute match observations were monitored during the 2016 and 2017 seasons (408 match observations, 11 [6]/player). HSR (≥3.47 m·s−1), VHSR (≥5.28 m·s−1), and SPR (≥6.25 m·s−1) were determined generically (GEN) in players using a 10-Hz global positioning system. HSR, VHSR, and SPR speed thresholds were also reconfigured according to player peak speed per se and in combination with the final velocity achieved in the 30:15 Intermittent Fitness Test (locomotor approach to establishing individual speed zones). On the morning following matches (match day [MD + 1]), players recorded subjective wellness ratings of fatigue and soreness using 7-point Likert scales. Results: Fatigue (−2.32; 95% CI, −2.60 to −2.03 au; P < .0001) and soreness (−2.05; 95% CI, −2.29 to −1.81; P < .0001) ratings worsened on MD + 1. Standardized unit changes in HSRGEN (fatigue: −0.05; 95% CI, −0.11 to 0.02 and soreness: −0.02, 95% CI, −0.07 to 0.04) and VHSRGEN (fatigue: −0.06; 95% CI, −0.12 to 0.00 and soreness: −0.04; 95% CI, −0.10 to 0.02) had no influence on wellness ratings at MD + 1. Individualized speed thresholds did not improve the model fit. Conclusions: Subjective ratings of fatigue and wellness are not sensitive to substantial within-player changes in match physical performance. HSR, VHSR, and SPR thresholds customized for individual players’ athletic qualities did not improve the dose–response relationship between external load and wellness ratings.
Effect of a Fatiguing Ultratrail on the Graded Energetically Optimal Stride Frequency
Authors: Vernillo Gianluca , Mater Adrien , Doucende Gregory , Cassirame Johan , Mourot Laurent 
Published: September 2020

Purpose: To study the consequences of a fatiguing ultratrail run of 6 hours on self-optimizing capability during uphill and downhill (DR) running. Methods: The authors collected temporal stride kinematics and metabolic data in 8 (experimental group) male runners before and after the ultratrail run and in 6 (control group) male ultramarathon runners who did not run but stayed awake and performed normal, daily physical activities avoiding strenuous exercises over the 6-hour period. For each subject, preferred and optimal stride frequencies were measured, where stride frequency was systematically varied above and below the preferred one (±4% and ±8%) while running 3 conditions on level, 5% uphill, or 5% DR in a randomized order. Results: Preferred and optimal stride frequencies across grade, group, and time showed no significant differences (P ≥ .184). Metabolic cost and the energetically optimum metabolic cost showed a grade × group × time interaction (P ≥ .011), with an ∼11% increase in the 2 variables only during the DR bouts (P ≥ .037). Conclusions: Despite maintaining similar dynamics of stride frequency adjustments during the DR bout, the experimental group was not able to optimize its gait. This suggests that the DR section of ultratrail runs can introduce a perturbing factor in the runners’ optimization process, highlighting the need for incorporating DR bouts in the training programs of ultratrail runners to minimize the deleterious effects of DR on the energetically optimal gait.
Promoting Physical Activity in Older Adults With Knee Osteoarthritis and Hypertension: A Randomized Controlled Trial
Authors: Schlenk Elizabeth A. , Fitzgerald G. Kelley , Rogers Joan C. , Kwoh C. Kent , Sereika Susan M. 
Published: September 2020

A 6-month self-efficacy intervention was compared with attention-control intervention on physical activity, clinical outcomes, and mediators immediate postintervention and 6-month postintervention in 182 older adults with knee osteoarthritis and hypertension using a randomized controlled trial design. The intervention group received six weekly individual physical therapy sessions for lower-extremity exercise and fitness walking and nine biweekly nurse telephone counseling sessions. The attention-control group received six weekly and nine biweekly nurse telephone sessions on health topics. Lower-extremity exercise was assessed by e-diary; fitness walking was assessed by accelerometer and e-diary; blood pressure was assessed by automated monitor; function was assessed by performance-based tests and questionnaires; and pain, self-efficacy, and outcome expectancy were assessed by questionnaires. Self-reported lower-extremity exercise and fitness walking, function, pain, self-efficacy, and outcome expectancy showed significant group or group by time effects favoring intervention. The intervention did not improve physical activity by accelerometer and blood pressure. Mean minutes of fitness walking fell short of the 150 min/week goal.
β-Cell function or insulin resistance was associated with the risk of type 2 diabetes among women with or without obesity and a history of gestational diabetes
Authors: Fan Yuxin , Wang Leishen , Liu Huikun , Zhang Shuang , Tian Huiguang , Shen Yun , Tuomilehto Jaakko , Yu Zhijie , Yang Xilin , Hu Gang , Liu Ming 
Published: September 2020

IntroductionTo evaluate the single association of postpartum β-cell dysfunction and insulin resistance (IR), as well as different combinations of postpartum β-cell dysfunction, IR, obesity, and a history of gestational diabetes mellitus (GDM) with postpartum type 2 diabetes risk.Research design and methodsThe study included 1263 women with prior GDM and 705 women without GDM. Homeostatic model assessment was used to estimate homeostatic model assessment of β-cell secretory function (HOMA-%β) and homeostatic model assessment of insulin resistance (HOMA-IR).ResultsMultivariable-adjusted ORs of diabetes across quartiles of HOMA-%β and HOMA-IR were 1.00, 1.46, 2.15, and 6.25 (ptrend<0.001), and 1.00, 2.11, 5.59, and 9.36 (ptrend<0.001), respectively. Women with IR only had the same diabetes risk as women with β-cell dysfunction only. Obesity, together with IR or β-cell dysfunction, had a stronger effect on diabetes risk. This stronger effect was also found for a history of GDM with IR or β-cell dysfunction. Women with three risk factors, including obesity, a history of GDM and β-cell dysfunction/IR, showed the highest ORs of diabetes.Conclusionsβ-cell dysfunction or IR was significantly associated with postpartum diabetes. IR and β-cell dysfunction, together with obesity and a history of GDM, had the highest ORs of postpartum diabetes risk.
Anti-inflammatory, antioxidant and renoprotective effects of SOCS1 mimetic peptide in the BTBR ob/ob mouse model of type 2 diabetes
Authors: Opazo-Ríos Lucas , Sanchez Matus Yenniffer , Rodrigues-Díez Raúl R , Carpio Daniel , Droguett Alejandra , Egido Jesús , Gomez-Guerrero Carmen , Mezzano Sergio 
Published: September 2020

IntroductionDiabetic nephropathy (DN) is the leading cause of chronic kidney disease worldwide. The Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway participates in the development and progression of DN. Among the different mechanisms involved in JAK/STAT negative regulation, the family of suppressor of cytokine signaling (SOCS) proteins has been proposed as a new target for DN. Our aim was to evaluate the effect of SOCS1 mimetic peptide in a mouse model of obesity and type 2 diabetes (T2D) with progressive DN.Research design and methodsSix-week-old BTBR (black and tan brachyuric) mice with the ob/ob (obese/obese) leptin-deficiency mutation were treated for 7 weeks with two different doses of active SOCS1 peptide (MiS1 2 and 4 µg/g body weight), using inactive mutant peptide (Mut 4 µg) and vehicle as control groups. At the end of the study, the animals were sacrificed to obtain blood, urine and kidney tissue for further analysis.ResultsTreatment of diabetic mice with active peptide significantly decreased urine albumin to creatinine ratio by up to 50%, reduced renal weight, glomerular and tubulointerstitial damage, and restored podocyte numbers. Kidneys from treated mice exhibited lower inflammatory infiltrate, proinflammatory gene expression and STAT activation. Concomitantly, active peptide administration modulated redox balance markers and reduced lipid peroxidation and cholesterol transporter gene expression in diabetic kidneys.ConclusionTargeting SOCS proteins by mimetic peptides to control JAK/STAT signaling pathway ameliorates albuminuria, morphological renal lesions, inflammation, oxidative stress and lipotoxicity, and could be a therapeutic approach to T2D kidney disease.
Determinants and correlates of adipose tissue insulin resistance index in Japanese women without diabetes and obesity
Authors: Kitaoka Kaori , Tsuboi Ayaka , Minato-Inokawa Satomi , Honda Mari , Takeuchi Mika , Yano Megumu , Kurata Miki , Wu Bin , Kazumi Tsutomu , Fukuo Keisuke 
Published: September 2020

IntroductionDeterminants and correlates of a novel index of adipose tissue insulin resistance (AT-IR) (the product of fasting insulin and free fatty acid concentrations) were investigated in Japanese women without diabetes and obesity.Research design and methodsCross-sectional associations of AT-IR with fat mass and distribution, and IR-related cardiometabolic variables were examined in 210 young and 148 middle-aged women whose average body mass index (BMI) was <23 kg/m2and waist was <80 cm. Multivariate linear regression analyses were used to identify most important determinants of AT-IR.ResultsYoung and middle-aged women did not differ in AT-IR (3.5±2.7 and 3.2±2.1, respectively). In both young and middle-aged women, AT-IR was positively associated with trunk/leg fat ratio, a sophisticated measure of abdominal fat accumulation, fasting plasma glucose (FPG), fasting triglycerides (FTG), serum alanine aminotransferase and γ-glutamyl-transpeptidase (all p<0.05). Furthermore, in middle-aged but not in young women, AT-IR showed positive associations with BMI, waist, fat mass index, low-density lipoprotein cholesterol, apolipoprotein B and systolic and diastolic blood pressure (BP) (all p<0.05). AT-IR showed no association with hemoglobin A1c, high-density lipoprotein (HDL) cholesterol and apolipoprotein A1 in two groups of women. On multivariate analysis including waist, FPG, FTG, HDL cholesterol and systolic BP as independent variables, FPG, FTG and HDL cholesterol emerged as independent determinants of AT-IR in young women (cumulative R2=0.141) and waist in middle-aged women (cumulative R2=0.056). In a model which included trunk/leg fat ratio instead of waist, trunk/leg fat ratio and systolic BP were determinants of AT-IR in middle-aged women (cumulative R2=0.093). Results did not alter in young women.ConclusionsAT-IR may be a simple and useful surrogate index of adipose tissue insulin resistance even in populations without diabetes and obesity.