bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carcinoembryonic Antigen, Squamous Cell Carcinoma Antigen, CYFRA 21-1, and Neuron-specific Enolase in Squamous Cell Lung Cancer Patients
Authors: Kulpa Jan , Wójcik Ewa , Reinfuss Marian , Kołodziejski Leszek 
Published: January 2020

AbstractBackground: Carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag), and CYFRA 21-1 are the most useful markers for non-small cell lung cancer (NSCLC), but neuron-specific enolase (NSE) is a tumor maker of choice for SCLC. The determination of NSE in NSCLC could allow selection of patients with neuroendocrine features. NSCLC patients whose tumors have neuroendocrine properties may be more responsive to chemotherapy; however, these tumors have been reported to be more aggressive. Tumor markers are not suitable for diagnosis; their principal applications are in monitoring of therapy and prognosis.Methods: Tumor markers were measured in 200 untreated patients with squamous cell lung cancer (SQC) and a reference group (n = 220; 124 healthy persons and 96 patients with nonmalignant lung disease). CEA and SCC-Ag were measured by microparticle enzyme immunoassays on Abbott AxSYM and IMx analyzers. CYFRA 21-1 and NSE were measured by electrochemiluminescence immunoassays on the Roche Elecsys 2010.Results: CEA, SCC-Ag, CYFRA 21-1, and NSE were increased above the cutoffs in 26%, 32%, 67%, and 28% of tested patients, respectively. The area under the ROC curve for CYFRA 21-1 was higher than those for CEA, SCC-Ag, and NSE (SQC vs controls). CYFRA 21-1 and CEA were significantly higher in advanced SQC than in early stages of disease (P &lt;0.0001 and P &lt;0.0004, respectively). In multivariate analysis of survival, CYFRA 21-1 was an independent but nonspecific prognostic factor in the operable group of SQC patients, whereas NSE was an independent prognostic factor in the advanced stages of disease.Conclusion: CYFRA 21-1 is an independent prognostic factor in earlier stages and NSE in the advanced stages of SQC.
Primary Standardization of Assays for Anticonvulsant Drugs: Comparison of Accuracy and Precision
Authors: Wilson John F , Watson Ian D , Williams John , Toseland Pat A , Thomson Alison H , Sweeney Grace , Smith Brian L , Sandle Lance N , Ramsey John D , Capps Nigel E 
Published: January 2020

AbstractBackground: The accuracy and precision of methods for the measurement of the anticonvulsants phenytoin, phenobarbital, primidone, carbamazepine, ethosuximide, and valproate in human serum were assessed in 297 laboratories that were participants in the United Kingdom National External Quality Assessment Scheme (UKNEQAS).Methods: We distributed lyophilized, serum-based materials containing low, medium, and high weighed-in concentrations of the drugs. The 297 participating laboratories received the materials on two occasions, 7 months apart. Expected concentrations were determined by gas chromatography or HPLC methods in five laboratories using serum-based NIST reference materials as calibrators.Results: In general, bias was consistent across concentrations for a method but often differed in magnitude for different drugs. Bias ranged from −1.9% to 8.6% for phenytoin, −2.7% to 3.1% for phenobarbital, −2.7% to 0.5% for primidone, −8.6% to 0.3% for carbamazepine, −5.6% to 2.0% for ethosuximide, and −7.2% to 0.1% for valproate. Intralaboratory sources of imprecision significantly exceeded interlaboratory sources for many drug/method combinations. The mean CVs for intra- and interlaboratory errors for the different drugs were 6.3–7.8% and 3.3–4.2%, respectively.Conclusions: For these long-established and relatively high-concentration analytes, the closed analytical platforms generally performed no better than open systems or chromatography, where use of calibrators prepared in house predominated. To improve the accuracy of measurements, work is required principally by the manufacturers of immunoassays to ensure minimal calibration error and to eliminate batch-to-batch variability of reagents. Individual laboratories should concentrate on minimizing dispensing errors.
Impact of Subgroup Prevalences on Partitioning of Gaussian-distributed Reference Values
Authors: Lahti Ari , Hyltoft Petersen Per , Boyd James C 
Published: January 2020

AbstractBackground: The aims of this report were to examine how unequal subgroup prevalences in the source population may affect reference interval partitioning decisions and to develop generally applicable guidelines for partitioning gaussian-distributed data.Methods: We recently proposed a new model for partitioning reference intervals when the underlying data distribution is gaussian. This model is based on controlling the proportions of the subgroup distributions that fall outside each of the common reference limits, using the distances between the reference limits of the subgroup distributions as functions to these proportions. We examine the significance of the unequal prevalence effect for the partitioning problem and quantify it for distance partitioning criteria by deriving analytical expressions to express these criteria as a function of the ratio of prevalences. An application example, illustrating various aspects of the importance of the prevalence effect, is also presented.Results: Dramatic shrinkage of the critical distances between reference limits of the subgroups needed for partitioning was observed as the ratio of prevalences, the larger one divided by the smaller one, was increased from unity. Because of this shrinkage, the same critical distances are not valid for all ratios of prevalences, but specific critical distances should be used for each particular value of this ratio. Although proportion criteria used in determining the need for reference interval partitioning are not dependent on the prevalence effect, this effect should be accounted for when these criteria are being applied by adjusting the sample sizes of the subgroups to make them correspond to the ratio of prevalences.Conclusions: The prevalences of subgroups in the reference population should be known and observed in the calculations for every reference interval study, irrespective of whether distance or proportion criteria are being used to determine the need for reference interval partitioning. We present detailed methods to account for the prevalences when applying each of these types of criteria. Analytical expressions for the distance criteria, to be used when high precision is needed, and approximate distances, to be used in practical work, are derived. General guidelines for partitioning gaussian distributed data are presented. Following these guidelines and using the new model, we suggest that partitioning can be performed more reliably than with any of the earlier models because the new model not only offers an improved correspondence between the critical distances and the critical proportions, but also accounts for the prevalence effect.
S100B Protein in Biological Fluids: A Tool for Perinatal Medicine
Authors: Michetti Fabrizio , Gazzolo Diego 
Published: January 2020

AbstractThe diagnosis of perinatal insults currently relies on adequate documentation of general medical and obstetric factors and on radiologic and laboratory assessments. The measurement of brain constituents such as S100B protein may offer an alternative and direct indicator of cell damage in the nervous system when clinical and radiologic assessments are still silent and has the additional advantage of providing a quantitative indicator of the extent of brain lesions. S100B protein has been measured by several immunoassays in biological fluids (i.e., cerebrospinal fluid, blood, amniotic fluid, and urine) from fetuses and newborns at high risk of perinatal brain damage. S100B protein in biological fluids increased at an early stage when standard monitoring procedures were still silent in the study populations that later developed brain damage. S100B concentration was also significantly correlated with the extent of brain lesions. S100B protein appears to satisfy the criteria for a marker for brain injuries in perinatal medicine: (a) simple to perform measurements with good reproducibility; (b) detection in a variety of biological fluids, possibly reducing perinatal stress related to testing; (c) possible use in longitudinal monitoring because of its 1-h half-life; and (d) well-established use as an early and quantitative marker of brain lesions/damage. Finally, because of the neurotrophic role putatively played by S100B, its measurement in biological fluids at pre-/perinatal ages makes it a candidate for the laboratory evaluation of brain maturation.
Analysis of Carnitine Biosynthesis Metabolites in Urine by HPLC–Electrospray Tandem Mass Spectrometry
Authors: Vaz Frédéric M , Melegh Bela , Bene Judit , Cuebas Dean , Gage Douglas A , Bootsma Albert , Vreken Peter , van Gennip Albert H , Bieber Loran L , Wanders Ronald JA 
Published: January 2020

AbstractBackground: We developed a method to determine the urinary concentrations of metabolites in the synthetic pathway for carnitine from N6-trimethyllysine and applied this method to determine their excretion in control individuals. In addition, we investigated whether newborns are capable of carnitine synthesis from deuterium-labeled N6-trimethyllysine.Methods: Urine samples were first derivatized with methyl chloroformate. Subsequently, the analytes were separated by ion-pair, reversed-phase HPLC and detected online by electrospray tandem mass spectrometry. Stable-isotope-labeled reference compounds were used as internal standards.Results: The method quantified all carnitine biosynthesis metabolites except 4-N-trimethylaminobutyraldehyde. Detection limits were 0.05–0.1 μmol/L. The interassay imprecision (CV) for urine samples with added compounds was 6–12%. The intraassay imprecision (CV) was 1–5% (3–10 μmol/L). Recoveries were 94–106% at 10–20 μmol/L and 98–103% at 100–200 μmol/L. The mean (SD) excretions of N6-trimethyllysine and 3-hydroxy-N6-trimethyllysine were 2.8 (0.8) and 0.45 (0.15) mmol/mol creatinine, respectively. γ-Butyrobetaine and carnitine excretions were more variable with values of 0.27 (0.21) and 15 (12) mmol/mol creatinine, respectively. After oral administration of deuterium-labeled N6-trimethyllysine, all urines of newborns contained deuterium-labeled N6-trimethyllysine, 3-hydroxy-N6-trimethyllysine, γ-butyrobetaine, and carnitine.Conclusions: HPLC in combination with electrospray ionization tandem mass spectrometry allows rapid determination of urinary carnitine biosynthesis metabolites. Newborns can synthesize carnitine from exogenous N6-trimethyllysine, albeit at a low rate.
Instruments for Self-Monitoring of Blood Glucose: Comparisons of Testing Quality Achieved by Patients and a Technician
Authors: Skeie Svein , Thue Geir , Nerhus Kari , Sandberg Sverre 
Published: January 2020

Abstract
Background: Instruments for self-monitoring of blood glucose (SMBG) are increasingly used by patients with diabetes. The analytical quality of meters in routine use is poorly characterized.
Methods: We compared SMBG performance achieved by patients and by a medical laboratory technician. Imprecision was calculated from duplicate measurements, and deviation as the difference between the first measurement and the mean of duplicate laboratory-method results (calibrated with NIST material). Analytical quality for five groups of SMBG instruments was compared with quality specifications for BG measurements. All participants completed a questionnaire assessing both SMBG training and use of the meters.
Results: We recruited 159 SMBG users from a hospital outpatient clinic and 263 others from 65 randomly selected general practices (total of 422). Most (two thirds) used insulin. CVs for the five meter types were 7%, 11%, 18%, 18%, and 20% in the hands of patients and 2.5–5.9% for the technician. For three of five meter types, patients’ BG measurements had larger deviations from the laboratory results than did the technician’s results. The technician’s performance could not predict the patients’. No instrument when used by patients (but two operated by the technician) met published quality specifications. The analytical quality of patients’ results was not related to whether they had chosen the instruments on advice from healthcare personnel (one-third of patients), were only self-educated in SMBG (50%), or performed SMBG fewer than seven times/week (62%).
Conclusions: The analytical quality of SMBG among patients was poorer than, and could not be predicted from, the performance of the meters in the hands of a technician. We suggest that new instruments be tested in the hands of patients who are trained on meter use in a routine way.
Reception Theory, New Humanism, and T. S. Eliot
Authors: Hiscock Matthew 
Published: January 2020

AbstractT. S. Eliot has been a major, if challenging, figure for students of reception and the Classical Tradition, and is implicated in an important debate on historicist versus aestheticist models of reception study. This article challenges assumptions about his position on, and practice of, reception. The politics implicit in theorists’ references to Eliot is teased out, and the position he took in response to inter-war New Humanism is shown to be predominantly historicist. An analysis of The Family Reunion (1939) then suggests that the Modernist-poetic approach he therefore took to the Oresteia broke so decisively with existing models of reception as to have called the fact of reception into question. The play is also shown to build on H.D.’s experiments in translation and to respond to Aeschylean receptions by Robinson Jeffers and Eugene O’Neill. It is further suggested that it anticipates several aspects of recent Reception Theory.
First Insights into the Chemical Ecology of an Invasive Pest: Olfactory Preferences of the Viburnum Leaf Beetle (Coleoptera: Chrysomelidae)
Authors: Desurmont Gaylord A , Morelon Stéphanie P G , Benrey Betty 
Published: January 2020

AbstractThe viburnum leaf beetle (VLB), Pyrrhalta viburni (Paykull), is an invasive chrysomelid in North America where it infests native Viburnum shrubs in woody areas and managed landscapes. Despite its invasive and destructive nature, little is known about the chemical ecology of this beetle, and efficient chemical lures for monitoring and trapping this insect have yet to be developed. Using two of the main host plants of VLB in its native range, Viburnum opulus L. (Caprifoliaceae) and V. lantana L., we examined the olfactory preferences of adult females of VLB under laboratory conditions and measured volatile emissions of Viburnum twigs with and without VLB damage. VLB females had a clear preference for V. opulus and V. lantana twigs compared to blank odor sources. In addition, twigs with foliar damage and fresh egg masses were found to be more attractive than noninfested twigs in V. opulus when VLB infestation was recent, but not when twigs had been infested for several weeks. Chemical analyses revealed consistent treatment-specific blends of compounds, which may be used for the elaboration of attractive lures. Future research should focus on the identification of these compounds and on exploring the olfactory preferences of VLB with Viburnum species present in North America.
Predictors of patient satisfaction and outpatient health services in China: evidence from the WHO SAGE survey
Authors: Zhang Hao , Wang Wenhua , Haggerty Jeannie , Schuster Tibor 
Published: January 2020

Abstract

Background
Patient satisfaction is an essential indicator in medical practise and research. To monitor the health and well-being of adult populations and the ageing process, the World Health Organization (WHO) has initiated the Study on Global AGEing and Adult Health (SAGE), compiling longitudinal information in six countries including China as one major data source.

Objective
The objective of this study was to identify potential predictors for patient satisfaction based on the 2007–10 WHO SAGE China survey.

Methods
Data were analysed using random forests (RFs) and ordinal logistic regression models based on 5774 responses to predict overall patient satisfaction on their most recent outpatient health services visit over the last 12 months. Potential predictor variables included access to care, costs of care, quality of care, socio-demographic and health care characteristics and health service features. Increase of the mean-squared error (incMSE) due to variable removal was used to assess relative importance of the model variables for accurately predicting patient satisfaction.

Results
The survey data suggest low frequency of dissatisfaction with outpatient services in China (1.8%). Self-reported treatment outcome of the respective visit of a care facility demonstrated to be the strongest predictor for patient satisfaction (incMSE +15%), followed by patient-rated communication (incMSE +2.0%), and then income, waiting time, residency and patient age. Individual patient satisfaction in the survey population was predicted with 74% accuracy using either logistic regression or RF.

Conclusions
Patients’ perceived outcomes of health care visits and patient communication with health care professionals are the most important variables associated with patient satisfaction in outpatient health services settings in China.
An Ancient Baboon Genome Demonstrates Long-Term Population Continuity in Southern Africa
Authors: Mathieson Iain , Abascal Federico , Vinner Lasse , Skoglund Pontus , Pomilla Cristina , Mitchell Peter , Arthur Charles , Gurdasani Deepti , Willerslev Eske , Sandhu Manj S , Dewar Genevieve 
Published: January 2020

Abstract
Baboons are one of the most abundant large nonhuman primates and are widely studied in biomedical, behavioral, and anthropological research. Despite this, our knowledge of their evolutionary and demographic history remains incomplete. Here, we report a 0.9-fold coverage genome sequence from a 5800-year-old baboon from the site of Ha Makotoko in Lesotho. The ancient baboon is closely related to present-day Papio ursinus individuals from southern Africa—indicating a high degree of continuity in the southern African baboon population. This level of population continuity is rare in recent human populations but may provide a good model for the evolution of Homo and other large primates over similar timespans in structured populations throughout Africa.
Effective Peer Leader Attributes for the Promotion of Walking in Older Adults
Authors: Kritz Marlene , Thøgersen-Ntoumani Cecilie , Mullan Barbara , McVeigh Joanne , Ntoumanis Nikos 
Published: January 2020

Abstract

Background and Objectives
Peer-led interventions are promising for the promotion of physical activity behavior in older adults. However, little is known about the attributes of effective older peer leaders in such intervention programs. The objective was to determine what older adults perceive to be effective peer leader attributes.

Research Design and Methods
A mixed-methods concurrent triangulation design was used. Participants, aged 60 years and older, were recruited from retirement villages and existing walking groups in Western Australia. They were predominantly white, Australian-born, female, healthy retirees. The sample consisted of four groups of older adults: those who had taken part in past peer-led walking programs (experienced walkers; n = 18), those interested in joining as walkers in a peer-led walking intervention (inexperienced walkers; n = 43), those interested to take on a peer leader role (inexperienced peer leaders; n = 25), and those who had already served as peer leaders (experienced peer leaders; n = 15). Questionnaires measured perceived effective leadership attributes, and physical activity was measured using ActivPAL devices (N = 101; Mage [SD] = 75.36 [7.59]). Semistructured interviews were conducted with the majority of participants (N = 68; Mage [SD] = 74.68 [7.78]).

Results
Overall, participants described an effective peer leader as optimistic, compassionate, and friendly, but differences in perceptions were apparent between the groups.

Discussion and Implications
Our findings advance knowledge about important characteristics of an effective older peer leader, which can inform peer leader training, recruitment of peer leaders, and future scale development.
Conflicting perceptions on the life-history covariates of density-dependent recruitment based on information-generating equations
Authors: Munyandorero Joseph 
Published: January 2020

Abstract
The stock–recruit steepness (h) and compensation ratio are alternative measures of the degree of density-dependent recruitment. They relate similarly to the longevity-related life-history traits (LRLHTs), but studies using information-generating equations and describing such relationships convey three conflicting perceptions. Perception 1 is that h and the compensation ratio decrease as longevity increases and are highest in short-lived, high-productivity species. This perception, usually believed to be inherent in the definition of steepness, is not substantiated by the very definition and, hence, theoretically is wrong. Perception 2 is that h and the compensation ratio increase with longevity and are highest in long-lived, low-productivity species; this perception may occur when the relationships between natural mortality and other LRLHTs are strong, and is most likely correct. Perception 3 is that h and the compensation ratio are independent of LRLHTs; this perception may be caused by the lack of the relationships between natural mortality and other LRLHTs, and is a distortion of perception 2.
Increasing use of the Impella®-pump in severe cardiogenic shock: a word of caution
Authors: Vasin Sergey , Philipp Alois , Floerchinger Bernhard , Rastogi Priyank , Lunz Dirk , Mueller Thomas , Schmid Christof , Camboni Daniele 
Published: January 2020

Abstract
Impella® pumps are increasingly utilized in patients in cardiogenic shock. We report on a case series where Impella support was insufficient, and a switch to venoarterial extracorporeal membrane oxygenation (VA ECMO) became necessary. ECMO patients with previous Impella devices were identified utilizing our institutional ECMO database. Since 2014, 10 patients with a mean age of 62 ± 3 years were identified. Despite correct placement of all Impella pumps, cardiogenic shock persisted with progressive multi-organ failure (Impella type 2.5/CP n = 6/4 patients). Femoro-femoral VA ECMO was implanted percutaneously on the contralateral side with the Impella initially left on standby but retracted into the descending aorta for transport reasons after a mean support time of 20 ± 8 h. All patients were able to unload their heart by left ventricular ejection with a blood pressure amplitude of 15 ± 3 mmHg on VA ECMO support. After VA ECMO implantation haemodynamic parameters improved significantly within 24 h of support (mean serum lactate levels decreased from 92 ± 17 to 44 ± 10 mg/dl, P = 0.031). Survival to hospital discharge was 70%. These data indicate that the Impella 2.5® and CP® might not be sufficient in profound cardiogenic shock. Comparative studies are necessary to specify which patient population benefits from which type of circulatory support.