bluuebunny/crossref_metadata_2025_split huggingface dataset:
Traditional foods and beverages in Eastern Tigray of Ethiopia
Authors: Tadesse Nigussie Seboka , Beyene Girum Faris , Hordofa Tesfaye Bekele , Hailu Ashenafi Ayenew 
Published: May 2020

AbstractEthiopia is a multiethnic country with diverse cultural practices to prepare foods and beverages having significant contributions in socioeconomic development. Contrary, comprehensive documentation of the products, associated knowledge, and their sociocultural roles is incomplete. In this survey, thirteen typical traditional foods (two fermented and eleven non-fermented) and five traditional beverages (four fermented and one non-fermented) were listed referring the local community of East Tigray. The noted traditional foods/beverages are known for their social and cultural values in the local community, and they are exercised following celebrity events. They are prepared often from barley (Hordeum vulgare), peas (Pisum sativum), wheat (Triticum aestivum), buckthorn (Rhamnus prinoides), maize (Zea mays), sorghum (Sorghum bicolor), and teff (Eragrostis tef). Some of the dishes/drinks such as Ambasha, Ebist, Gaa’t, Tihelo, Beso, Zikizuko, Abaekah, Duqua, Suwa, Mes, and Tahetah are well-acknowledged for their health and economic benefits. Others, like Birkuta and sola, are used to survive a long journey and looming drought. To conclude, this article presents a list of traditional foods and beverages which are potentially applicable in modern food or beverage industries and can contribute for global healthy diet.
Male Sex Hormone affection in patients with Tramadol dependance
Authors: Darweesh Alaa Eldin M. , Khalifa Hossam , Gabra Romany H. , Fahim Mena M. 
Published: May 2020

Abstract
Background
Tramadol is a centrally active synthetic opioid analgesic that is used extensively among Egyptian males for premature ejaculation as a self-medication.
The present work conducted to outline male sex hormone affection in patients with tramadol dependence and its relation to sexual satisfaction.

Results
Tramadol-dependent patients have significantly lower mean serum level of testosterone compared to the control group and lower mean Sexual Satisfaction Scale than the control group.

Conclusions
The results provide evidence for the association between male sex hormone suppression in patients with tramadol dependence with high Addiction Severity Index of tramadol dependence.
Schwann cell reprogramming into repair cells increases miRNA-21 expression in exosomes promoting axonal growth
Authors: López-Leal Rodrigo , Díaz-Viraqué Florencia , Catalán Romina J. , Saquel Cristian , Enright Anton , Iraola Gregorio , Court Felipe A. 
Published: May 2020

ABSTRACT
Functional recovery after peripheral nerve damage is dependent on the reprogramming of differentiated Schwann cells (dSCs) into repair Schwann cells (rSCs), which promotes axonal regeneration and tissue homeostasis. Transition into a repair phenotype requires expression of c-Jun and Sox2, which transcriptionally mediates inhibition of the dSC program of myelination and activates a non-cell-autonomous repair program, characterized by the secretion of neuronal survival and regenerative molecules, formation of a cellular scaffold to guide regenerating axons and activation of an innate immune response. Moreover, rSCs release exosomes that are internalized by peripheral neurons, promoting axonal regeneration. Here, we demonstrate that reprogramming of Schwann cells (SCs) is accompanied by a shift in the capacity of their secreted exosomes to promote neurite growth, which is dependent on the expression of c-Jun (also known as Jun) and Sox2 by rSCs. Furthermore, increased expression of miRNA-21 is responsible for the pro-regenerative capacity of rSC exosomes, which is associated with PTEN downregulation and PI3-kinase activation in neurons. We propose that modification of exosomal cargo constitutes another important feature of the repair program of SCs, contributing to axonal regeneration and functional recovery after nerve injury.
Naturally occurring and tumor-associated variants of RNF167 promote lysosomal exocytosis and plasma membrane resealing
Authors: Nair Sreeja V. , Narendradev Nikhil Dev , Nambiar Rithwik P. , Kumar Rakesh , Srinivasula Srinivasa M. 
Published: May 2020

ABSTRACT
Lysosomal exocytosis and resealing of damaged plasma membrane are essential for cellular homeostasis and tumor invasion. However, very little is known of the molecular machinery that regulates these physiological processes. Moreover, no mutations in any of the known regulators of lysosomal exocytosis in primary tumors of patients have been characterized. Here we demonstrate that RNF167-a, a lysosomal-associated ubiquitin ligase, negatively regulates lysosomal exocytosis by inducing perinuclear clustering of lysosomes. Importantly, we also characterized a set of novel natural mutations in RNF167-a, which are commonly found in diverse tumor types. We found that RNF167-a-K97N mutant, unlike the wild type, localizes in the cytoplasm and does not promote perinuclear lysosomal clustering. Furthermore, cells expressing RNF167-a-K97N exhibit dispersed lysosomes, increased exocytosis and enhanced plasma membrane repair. Interestingly, these functional features of RNF167-a-K97N were shared with a naturally occurring short version of RNF167 (isoform RNF167-b). In brief, the results presented here reveal a novel role of RNF167-a, as well as its natural variants RNF167-a-K97N and RNF167-b, as an upstream regulator of lysosomal exocytosis and plasma membrane resealing.
Sensitive detection of protein ubiquitylation using a protein fragment complementation assay
Authors: Le Boulch Marie , Brossard Audrey , Le Dez Gaëlle , Léon Sébastien , Rabut Gwenaël 
Published: May 2020

ABSTRACT
Ubiquitylation is a reversible post-translational protein modification that regulates a multitude of cellular processes. Detection of ubiquitylated proteins is often challenging because of their low abundance. Here, we present NUbiCA, a sensitive protein-fragment complementation assay to facilitate the monitoring of ubiquitylation events in cultured cells and model organisms. Using yeast as a model system, we demonstrate that NUbiCA enables accurate monitoring of mono- and polyubiquitylation of proteins expressed at endogenous levels. We also show that it can be applied to decipher the topology of ubiquitin conjugates. Moreover, we assembled a genome-wide collection of yeast strains ready to investigate the ubiquitylation of proteins with this new assay. This resource will facilitate the analysis of local or transient ubiquitylation events that are difficult to detect with current methods.
Laminin-511 and α6 integrins regulate the expression of CXCR4 to promote endothelial morphogenesis
Authors: Xu Hao , Pumiglia Kevin , LaFlamme Susan E. 
Published: May 2020

ABSTRACT
During angiogenesis, endothelial cells engage components of the extracellular matrix through integrin-mediated adhesion. Endothelial expression of laminin-411 and laminin-511 is known to promote vessel stability. However, little is known about the contribution of these laminins to endothelial morphogenesis. We used two organotypic cell culture angiogenesis assays, in conjunction with RNAi approaches, to demonstrate that depletion of either the α4 chain of laminin-411 (LAMA4) or the α5 chain of laminin-511 (LAMA5) from endothelial cells inhibits sprouting and tube formation. Depletion of α6 (ITGA6) integrins resulted in similar phenotypes. Gene expression analysis indicated that loss of either laminin-511 or α6 integrins inhibited the expression of CXCR4, a gene previously associated with angiogenic endothelial cells. Pharmacological or RNAi-dependent inhibition of CXCR4 suppressed endothelial sprouting and morphogenesis. Importantly, expression of recombinant CXCR4 rescued endothelial morphogenesis when α6 integrin expression was inhibited. Additionally, the depletion of α6 integrins from established tubes resulted in the loss of tube integrity and laminin-511. Taken together, our results indicate that α6 integrins and laminin-511 can promote endothelial morphogenesis by regulating the expression of CXCR4 and suggest that the α6-dependent deposition of laminin-511 protects the integrity of established endothelial tubes.
A simple device to immobilize protists for electrophysiology and microinjection
Authors: Kulkarni Anirudh , Elices Irene , Escoubet Nicolas , Pontani Léa-Laetitia , Prevost Alexis Michel , Brette Romain 
Published: May 2020

ABSTRACT
We present a simple device to mechanically immobilize motile cells such as ciliates. It can be used in particular for intracellular electrophysiology and microinjection. A transparent filter with holes smaller than the specimen is stretched over an outlet. A flow is induced by either a peristaltic pump or a depressurized tank, mechanically entraining cells to the bottom, where they are immobilized against the filter. The cells start swimming again as soon as the flow is stopped. We demonstrate the device by recording action potentials in Paramecium and injecting a fluorescent dye into the cytosol.
Hydroxyapatite Formation from Octacalcium Phosphate and Its Related Compounds: A Discussion of the Transformation Mechanism
Authors: Yokoi Taishi , Goto Tomoyo , Kato Takeharu , Takahashi Seiji , Nakamura Jin , Sekino Tohru , Ohtsuki Chikara , Kawashita Masakazu 
Published: May 2020

Abstract
Octacalcium phosphate (OCP), which has a layered structure composed of an apatitic layer and a hydrated layer, is a precursor of hydroxyapatite (HAp). Although the topotactic transformation of OCP to HAp is a well-known phenomenon, its mechanism remains unclear. In this study, to clarify the role of the OCP hydrated layer in the mentioned transformation, we investigated the behavior of the transformation of OCP with hydrated layers of different thicknesses to HAp under hydrothermal conditions. We used three types of samples: plain OCP (Pure-OCP), OCP with incorporated succinate ions (Suc-OCP), and OCP with incorporated suberate ions (Sub-OCP). We found that all three OCP types transformed topotactically into HAp. The order of reactivity was Sub-OCP &gt; Suc-OCP &gt; Pure-OCP, which corresponds to the thickness of the hydrated layer. Since the transformation involves a compositional change, our finding implies that the hydrated layer acts as a diffusion (mass transfer) passage for dicarboxylate ions and hydrogen phosphate ions located within it, which move outside the crystal during the topotactic transformation. Our results thus contribute to a better understanding of the topotactic transformation mechanism of OCPs to HAp.
Measuring extraordinary experiences and beliefs: A validation and reliability study
Authors: Wahbeh Helané , Yount Garret , Vieten Cassandra , Radin Dean , Delorme Arnaud 
Published: May 2020

Background: Belief in the paranormal is widespread worldwide. Recent surveys suggest that subjective experiences of the paranormal are common. A concise instrument that adequately evaluates beliefs as distinct from experiences does not currently exist. To address this gap, we created the Noetic Experiences and Beliefs Scale (NEBS) which evaluates belief and experience as separate constructs. Methods: The NEBS is a 20-item survey with 10 belief and 10 experience items rated on a visual analog scale from 0-100. In an observational study, the survey was administered to 361 general population adults in the United States and a subsample of 96 one month later. Validity, reliability and internal consistency were evaluated. A confirmatory factor analysis was conducted to confirm the latent variables of belief and experience. The survey was then administered to a sample of 646 IONS Discovery Lab participants to evaluate divergent validity and confirm belief and experience as latent variables of the model in a different population. Results: The NEBS demonstrated convergent validity, reliability and internal consistency (Cronbach’s alpha Belief 0.90; Experience 0.93) and test-retest reliability (Belief: r = 0.83; Experience: r = 0.77). A confirmatory factor analysis model with belief and experience as latent variables demonstrated a good fit. The factor model was confirmed as having a good fit and divergent validity was established in the sample of 646 IONS Discovery Lab participants. Conclusions: The NEBS is a short, valid, and reliable instrument for evaluating paranormal belief and experience.
Optimization of glutaminase-free L-asparaginase production using mangrove endophytic Lysinibacillus fusiformis B27
Authors: Prihanto Asep Awaludin , Yanti Indah , Murtazam Mohammad Achsanil , Jatmiko Yoga Dwi 
Published: May 2020

Background: The mangrove, Rhizophora mucronata, an essential source of endophytic bacteria, was investigated for its ability to produce glutaminase-free L-asparaginase. The study aimed to obtain glutaminase-free L-asparaginase-producing endophytic bacteria from the mangrove and to optimize enzyme production. Methods: The screening of L-asparaginase-producing bacteria used modified M9 medium. The potential producer was further analyzed with respect to its species using 16S rRNA gene sequencing. Taguchi experimental design was applied to optimize the enzyme production. Four factors (L-asparagine concentration, pH, temperature, and inoculum concentration) were selected at four levels. Results: The results indicated that the endophytic bacteria Lysinibacillus fusiformis B27 isolated from R. mucronata was a potential producer of glutaminase-free L-asparaginase. The experiment indicated that pH 6, temperature at 35°C, and inoculum concentration of 1.5% enabled the best production and were essential factors. L-asparagine (2%) was less critical for optimum production. Conclusions: L. fusiformis B27, isolated from Rhizophora mucronata, can be optimized for L-ASNase enzyme production using optimization factors (L-ASNase, pH, temperature, and inoculum), which can increase L-ASNase enzyme production by approximately three-fold.
Efficient Inaccuracy: User-Generated Information Sharing in a Queue
Authors: Wang Jianfu , Hu Ming 
Published: May 2020

We study a service system that does not have the capability of monitoring and disclosing its real-time congestion level. However, the customers can observe and post their observations online, and future arrivals can take into account such user-generated information when deciding whether to go to the service facility. We perform pairwise comparisons of the shared, full, and no queue-length information structures in terms of social welfare. Perhaps surprisingly, we show that the shared queue-length information may provide greater social welfare than full queue-length information when the hassle cost of the customers entering the service facility falls into some ranges, and the shared and full queue-length information structures always generate greater social welfare than no queue-length information. Therefore, the discrete disclosure of congestion through user-generated sharing can lead to as much, or even greater, social welfare as the continuous stream of real-time queue-length information disclosure and always generates greater social welfare than no queue-length information disclosure at all. These results imply that a little shared queue-length information—inaccurate and lagged—can go a long way and that it may be more socially beneficial to encourage the sharing of user-generated information among customers than to provide them with full real-time queue-length information. This paper was accepted by Terry Taylor, operations management.
A simple method to secure data-driven improvement of perioperative care
Authors: Jakobsen Dorthe Hjort , Kehlet Henrik 
Published: May 2020

Background: Enhanced recovery after surgery (ERAS) programmes have been adopted to a varying degree by most surgical departments, not only in Denmark, but worldwide. Aims: To report the process from a local ERAS unit in a tertiary university hospital to accelerate implementation of ERAS programmes in all surgical specialties. Methods: All surgical departments receive twice-yearly procedure-specific data on length of stay (LOS), readmission rates and death within 30 days, based on surgical codes and the Danish National Patient Register. The ERAS unit and clinical experts review data followed by a clinical audit where appropriate. Findings: Setting up data presentation for clinical and nurse leaders has documented progress in implementing ERAS. The combination of outcome data, together with audits have been essential. Conclusion: The local ERAS unit has been shown to accelerate implementation of ERAS programmes in all surgical specialties, facilitated by procedure-specific LOS and re-admission data, combined with audit data.
<p><strong>The decline of the charismatic <em>Parnassius mnemosyne</em> (L.) (Lepidoptera: Papilionidae) in a Central Italy national park: a call for urgent actions</strong></p>
Authors: CINI ALESSANDRO , BARBERO FRANCESCA , BONELLI SIMONA , BRUSCHINI CLAUDIA , CASACCI LUCA PIETRO , PIAZZINI SANDRO , SCALERCIO STEFANO , DAPPORTO LEONARDO 
Published: May 2020

Here we report the strong decline of a population of the endangered species Parnassius mnemosyne (Linnaeus, 1758) (Lepidoptera: Papilionidae) in the National Park of Foreste Casentinesi, Monte Falterona and Campigna (Italy). We compared historical presence data (before 1969) with current data from two years of monitoring (2018–2019) and provided evidence of a drastic reduction in the number of sites inhabited by the species. A preliminary assessment suggested that the population of P. mmemosyne occurring in the Park is limited to a few individuals. We argue that the population of this iconic and charismatic butterfly is at the verge of extinction in this National Park, probably because of a combination of habitat loss (i.e. decrease in size and number of areas of open grassland on the mountain belt) and climatic changes. Being one of the few populations of P. mnemosyne in the Northern Apennines, the implementation of protection measures is a high conservation priority. Several other butterfly species on the Italian mountains that are facing the same survival challenges would also benefit from the establishment of conservation actions aimed at improving habitat quality for P. mnemosyne. Present article is meant to call for action researchers, stakeholders, and especially decision-makers in order to increase the efforts to upturn the evident decline in abundance of this population. Key words: biodiversity conservation, butterflies, habitat loss, habitat management, Lepidoptera, Parnassinae
Flight model characterization of the wide-field off-axis telescope for the MATS satellite
Authors: Park Woojin , Hammar Arvid , Pak Soojong , Chang Seunghyuk , Gumbel Jörg , Megner Linda , Christensen Ole Martin , Rouse Jordan , Kim Dae Wook 
Published: May 2020

We present optical characterization, calibration, and performance tests of the Mesospheric Airglow/Aerosol Tomography Spectroscopy (MATS) satellite, which for the first time, to the best of our knowledge, for a satellite, applies a linear-astigmatism-free confocal off-axis reflective optical design. Mechanical tolerances of the telescope were investigated using Monte Carlo methods and single-element perturbations. The sensitivity analysis results indicate that tilt errors of the tertiary mirror and a surface RMS error of the secondary mirror mainly degrade optical performance. From the Monte Carlo simulation, the tolerance limits were calculated to

±

0.5

m
m

,

±

1

m
m

, and

±

0.15
∘

for decenter, despace, and tilt, respectively. We performed characterization measurements and optical tests with the flight model of the satellite. Multi-channel relative pointing, total optical system throughput, and distortion of each channel were characterized for end-users. Optical performance was evaluated by measuring the modulation transfer function (MTF) and point spread function (PSF). The final MTF performance was 0.25 MTF at 20 lp/mm for the ultraviolet channel (304.5 nm), and 0.25–0.54 MTF at 10 lp/mm for infrared channels. The salient fact of the PSF measurement of this system is that there is no noticeable linear astigmatism detected over a wide field of view (

5.67
∘

×

0.91
∘

). All things considered, the design method showed great advantages in wide field of view observations with satellite-level optical performance.
Polarization splitting directional coupler using tilted subwavelength gratings
Authors: Luque-González José Manuel , Herrero-Bermello Alaine , Ortega-Moñux Alejandro , Sánchez-Rodríguez Marina , Velasco Aitor V. , Schmid Jens H. , Cheben Pavel , Molina-Fernández Íñigo , Halir Robert 
Published: May 2020

On-chip polarization splitters are key elements for coherent optical communication systems and polarization diversity circuits. These devices are often implemented with directional couplers that are symmetric for one polarization and strongly asymmetric for the other polarization. To achieve this asymmetry, highly dissimilar waveguides are used in each coupler arm, often requiring additional material layers or etch steps. Here we demonstrate polarization splitting with a directional coupler composed of two fully etched subwavelength waveguides, differing only in the tilt angle of the silicon segments. Our device exhibits deep-UV compatible feature sizes, is 14 µm long, and covers a 72 nm bandwidth with insertion losses below 1 dB and an extinction ratio in excess of 15 dB.
Synchrotron-based VUV excitation-induced ultrahigh quality cool white
light luminescence from Sm-doped ZnO
Authors: Kaur Puneet , Kriti  , Rahul  , Kaur Simranpreet , Kandasami Asokan , Singh Davinder Paul 
Published: May 2020

We report that the rare-earth (RE) ion, Sm-doped ZnO, acts as white
light emitting vacuum ultraviolet (VUV) phosphors and possesses an
ultrahigh color rendering index (CRI) and color quality scale (CQS).
The VUV-excited emission spectra measured from the synchrotron source
reveal the emergence of multi-color emission bands in the visible-IR
region and substantially depend on the concentration of

S
m

3
+

ions. A mechanism is proposed to
elucidate the origin behind the high-energy bandgap excitation of the
host charge carrier and subsequent energy transfer to the

S
m

3
+

states leading to additional
green-yellow-orange emission bands of

S
m

3
+

(
4

G

5

/

2

→
6

H

J

(

J

=

5

/

2

,

7

/

2

,

a
n
d

9

/

2

)
)

. High-quality cool white light
(correlated color temperature 5600 K) having CIE coordinates (0.33,
0.35) with a CRI as high as 95.89 and a CQS value of 94.49 is achieved
for

Z
n

0.985

S
m

0.015

O

under synchrotron VUV radiations.
This Letter demonstrates that RE activated ZnO-based phosphors are
expected to be a promising candidate in solid state lighting, as well
as plasma display devices.
Air-stable perovskite photovoltaic cells with low temperature deposited NiOx as an efficient hole-transporting material
Authors: Mahmud Hasan A. K. , Raifuku Itaru , Amin N. , Ishikawa Yasuaki , Sarkar D. K. , Sobayel K. , Karim Mohammad R. , Ul-Hamid Anwar , Abdullah H. , Shahiduzzaman Md. , Uraoka Yukiharu , Sopian Kamaruzzaman , Akhtaruzzaman Md. 
Published: May 2020

The electron-beam physical vapor deposition (EBPVD) technique was selected for nickel oxide (NiOx) film deposition at room temperatures. NiOx film (18 nm thick) was deposited as a hole transporting material (HTM) for inverted perovskite solar cells (PSCs) onto a fluorine-doped tin oxide (FTO)-coated glass substrate at a chamber vacuum pressure of 4.6×104 Pa. PSCs were fabricated as a glass/FTO/NiOx(HTM)/CH3NH3PbI3/PC61BM/BCP/Ag structure with as-deposited and annealed (500 °C for 30 min) NiOx films. Under 100 mW cm-2 illumination, as-deposited and annealed NiOx as HTM in PSCs (0.16 cm2) showed a high-power conversion efficiency (PCE) of 13.20% and 13.24%, respectively. The as-deposited and annealed PSCs retained 72.2% and 76.96% of their initial efficiency in ambient conditions, correspondingly. This study highlights the possibility of achieving highly crystalline and finely disseminated NiOx films by EBPVD for fabricating efficient inverted PSCs.