bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete mitogenome of Olidiana ritcheriina (Hemiptera: Cicadellidae) and phylogeny of Cicadellidae
Authors: Wang Xian-Yi , Wang Jia-Jia , Fan Zhi-Hua , Dai Ren-Huai 
Published: November 2019

Background
Coelidiinae, a relatively large subfamily within the family Cicadellidae, includes 129 genera and ∼1,300 species distributed worldwide. However, the mitogenomes of only two species (Olidiana sp. and Taharana fasciana) in the subfamily Coelidiinae have been assembled. Here, we report the first complete mitogenome assembly of the genus Olidiana.

Methods
Specimens were collected from Wenxian County (Gansu Province, China) and identified on the basis of their morphology. Mitogenomes were sequenced by next-generation sequencing, following which an NGS template was generated, and this was confirmed using polymerase chain reaction and Sanger sequencing. Phylogenic trees were constructed using maximum likelihood and Bayesian analyses.

Results
The mitogenome of O. ritcheriina was 15,166 bp long, with an A + T content of 78.0%. Compared with the mitogenome of other Cicadellidae sp., the gene order, gene content, gene size, base composition, and codon usage of protein-coding genes (PCGs) in O. ritcheriina were highly conserved. The standard start codon of all PCGs was ATN and stop codon was TAA or TAG; COII, COIII, and ND4L ended with a single T. All tRNA genes showed the typical cloverleaf secondary structure, except for trnSer, which did not have the dihydrouridine arm. Furthermore, the secondary structures of rRNAs (rrnL and rrnS) in O. ritcheriina were predicted. Overall, five domains and 42 helices were predicted for rrnL (domain III is absent in arthropods), and three structural domains and 27 helices were predicted for rrnS. Maximum likelihood and Bayesian analyses indicated that O. ritcheriina and other Coelidiinae members were clustered into a clade, indicating the relationships among their subfamilies; the main topology was as follows: (Deltocephalinae + ((Coelidiinae + Iassinae) + ((Typhlocybinae + Cicadellinae) + (Idiocerinae + (Treehopper + Megophthalminae))))). The phylogenetic relationships indicated that the molecular taxonomy of O. ritcheriina is consistent with the current morphological classification.
Assessment of Cardio-metabolic Risk Factors of Pre-diabetes/Diabetes among University Students: A Cross Sectional-study at Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Authors: Afranie Bright Oppong , Donkor Sampson , Koffie Simon , Segbefia Selorm Philip , Gyapong Joseph Badu , Amoah Beatrice , Nimoh Kwabena Fofie , Sampson Joseph Entwi 
Published: November 2019

Introduction: Diabetes is one of the most common chronic diseases globally and its complications include retinopathy, nephropathy and neuropathy. Early detection of the risk factors and management of pre-diabetes can however reduce the risk of developing diabetes-related complications.
Objective: This study was undertaken to assess cardio-metabolic risk factors for diabetes among undergraduate students at KNUST.
Method: The study was carried out at the Kwame Nkrumah University of Science and Technology at Kumasi, from January 2017 to March 2017. Questionnaires were administered to 500 participants (45% males and 55% females) to obtain demographic and anthropometric data, information on physical activity, and knowledge on diabetes. About 5 ml of venous blood was taken from participants after an overnight fast to determine fasting plasma glucose (FPG) and lipid profile.
Results: The mean age of participants were 21±2.40 years and 19.24±2.42 years for male and female respectively. Prevalence rate of pre-diabetes was 5% in this study. Mean values of systolic pressure (p<0.001) and blood glucose level (p=0.042) were significantly higher in men than the females. There was a significant association between the total cholesterol and the fasting blood sugar levels among participants (p=0.045). LDL-cholesterols concentrations were also significantly associated with blood sugar levels among participants (p<0.001). Risk factors such physical exercise (p<0.001) and family history (p<0.001) if diabetes were significantly associated with fasting blood glucose levels of participants. Obesity was no associated with serum glucose levels among participants in this study (p=0.533). HDL-cholesterol was inversely correlated with Fasting blood sugar even though it was no significant (r=-0.240, p=0.430).
Conclusion: The prevalence rate of pre-diabetes was 5% in this study. Education about the disease as well as appropriate modification of lifestyle can help reduce the incidence of diabetes and its associated risk factors.
Evaluation of Oxidative Stress Markers and Hormonal Profiles in Women Diagnosed with Infertility in Port Harcourt
Authors: Lawrence Olofinshawo, Olanrewaju , Samuel Bartimaeus, Ebirien-Agana , Gabriel Davies, Tamuno-Emine 
Published: November 2019

Aim: The study evaluates the contribution of oxidative stress and fertility hormones to female infertility in Port Harcourt.
Methodology: A total of 140 women aged 15 – 49 years consisting 70 apparently healthy infertile women who are attending diagnostic fertility clinics in Port Harcourt as test subjects and 70 age-matched healthy fertile women as control were recruited. Subjects were recruited using structured questionnaires after given their informed consent. The levels of Malondialdehyde, total antioxidant capacity, lipid peroxidation index, follicle stimulating hormone, luteinizing hormone, prolactin, progesterone and estrogen of infertile and the fertile (control) subjects were determined by standard procedures.
Results: There was statistically significant increase in lipid peroxidation index in the test subjects than in the fertile group (p<0.05). Total antioxidant capacity showed a statistically reduced value in the test subjects than in the control at p<0.05. Lipid peroxidation index was significantly increased in test subjects exposed to oxidant agents like alcohol, infections and ulcer than their counterparts who were not exposed to any of the agents (p<0.05). Also significantly elevated lipid peroxidation was observed in test subjects with normal hormone levels compared to those with hormone imbalance (p<0.05).
Conclusion: The outcome of this study suggests that the infertility being experienced by some of the infertile women in Port Harcourt are due not only to endocrine dysfunction, but some order conditions that induce oxidative stress. Thus investigation of oxidative parameters is highly suggested as an adjunct for effective management of unexplained infertility in women. Further studies on estimation of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GHR) are recommended to validate reliability.
Biochemical Changes Associated with Consumption (by Rats) of “Garri” Processed by Traditional and Instant Mechanical Methods
Authors: Airaodion A. I. , Ene A. C. , Ogbuagu E. O. , Okoroukwu V. N. , Ekenjoku J. A. , Ogbuagu U. 
Published: November 2019

Aim: This study aimed to investigate the biochemical changes in rats, associated with the consumption of “garri” (roasted (fried) fermented cassava flour) processed by traditional and instant mechanical methods.
Methods: Cassava samples obtained from the International Institute of Tropical Agriculture (IITA), Ibadan were processed using traditional and instant mechanical methods. Fifteen adult male Wistar rats were purchased from the Animal Holding Unit of the Department of Physiology, University of Ibadan, Nigeria, with body weights between 100 and 120 g. They were acclimatized for 7 days during which they were fed ad libitum with standard feed and drinking water. They were randomly divided into 3 groups of 5 rats each. Rats in group A were fed with pure standard rat chow. Rats in group B were fed with garri processed by instant mechanical method (fermented ≤ 24 hours before roasting) while those in group C were fed with garri processed by the traditional method (fermented ≥ 72 hours before roasting). After 28 days of feeding, the animals were fasted overnight and anaesthetized using diethyl ether. Blood samples were collected by cardiac puncture. Hepatic and renal indices were determined using standard methods.
Results: Mild perturbations were observed in the liver and renal biochemical parameters when animals fed with garri processed by the traditional method were compared with that fed garri processed by instant mechanical method and control diet. These perturbations were more severe in animals fed with garri processed by instant mechanical method.
Conclusion: In this study, both garri samples did not cause hepatic nor renal damage but mild perturbation of biochemical parameters were observed. These perturbations were more severe in animals fed with garri processed by instant mechanical method. This could be attributed to the high cyanide content in it.
Paternal Contribution to Banana (Musa sapientum L.) & Plantain (Musa paradisiaca L.) Progenies & Progeny Ploidy Composition in a Polycross Mating System Using RAPD & Flow Cytometry
Authors: Wilson Victoria , Tenkouano Abdou , Pillay Michael 
Published: November 2019

Aims: A 4x – 2x polycross mating design of 4 tetraploid female parents was established to determine paternal contributions of 3 diploid male parents to resulting progenies, their ploidy composition and genetic diversity of synthetic hybrids.
Study Design: The polycross mating design comprised 2 blocks having both maternal and paternal selections, with seed parents replicated at 12 plants per clone. Each crossing block had 31 plants of each of the three male parents.
Place and Duration of Study: International Institute of Tropical Agriculture (IITA) High Rainfall Station, Onne (4º51’N, 7º03’E, 10 m above sea level), Rivers State, South-South Nigeria for a period of 24 months.
Methodology: At maturity of maternal parents (TMPx 2796-5; TMPx 1658-4; TMPx 5511-2; and TMPx 7152-2), fruit bunches were harvested, ripened and the seeds extracted. Hard seeds obtained were germinated in vivo in seed trays and emerging seedlings transplanted to perforated nursery bags. At 12 weeks, DNA was extracted from candle leaf for RAPD analysis of 80 progenies and the 3 pollen parents. Ploidy status of progenies was determined using flow cytometry method.
Results: There was significant unequal paternal contribution to Musa polycross progenies with 3 maternal parents; TMPx 2796-5, TMPx 5511-2, and TMPx 1658-4. Two of the 3 paternal parents had progenies with all 4 maternal parents while TMB2x 5105-1 did not have any progeny with TMPx 2796-5. Progenies exhibited 4 ploidy levels with frequency differing with each female parent: TMPx 7152-2 produced 100% 3x progeny; TMPx 5511-2, 63% 3x and 37% 2x; TMPx 2796-5, 91% 3x and 9% 2x and TMPx 1658-4, 82% 3x, 9% 2x, 6% 4x and 3% 5x. The 5x progeny was recorded in the first ratoon crop. The second ratoon crop had only triploids.
Conclusion: The high frequency of 3x progenies from all maternal types in this study, suggests the effectiveness of the polycross mating design in Musa improvement.
Emotional Intelligence and Social Abilities are a Priority in Asperger’s Adolescents
Authors: Tapia-Pancardo Diana Cecilia 
Published: November 2019

Objective: To analyze the influence of Emotional Intelligence and Social Abilities development as interventions in an Asperger’s adolescent, to facilitate his interpersonal relationships.
Materials and Method: Qualitative and ethnographic methodology with intervention in different settings like personal, family and scholar; one Asperger’s adolescent, his mother, his teachers and school authorities informed through deep semi-structured interviews, with a 5 year follow up. Participants signed an informed consent and the qualitative analysis was according to de Souza Minayo.
Results: Two categories emerged: 1. Emotional impact in his parents due to lack of empathy in their son. 2. Language problems that impair his communication with peers and teachers.
Conclusion: Interventions favored identification and thinking of self and others’ emotions, regulate negative behaviors, increase of assertive and better interpersonal relationships.
Homolateral Hip Dislocation, Floating Hip Injury, and Floating Knee Injury: A Unique Presentation of a Rare Injury
Authors: Li Dongzhe , Fang Yue , Xiang Zhou 
Published: November 2019

Introduction:
Floating joint injury is an unusual injury pattern that is hard to deal with and often caused by high-energy trauma. In this report, a patient had a homolateral floating hip injury (FHI), floating knee injury (FKI), and hip dislocation at our hospital, and there was no case reported before.

Case Presentation:
A 48-year-old driver who encountered a traffic accident was seen. Radiologic examination revealed acetabulum comminuted fractures and hip joint posterior dislocation with some fracture pieces in the joint space on the left side. The femur, tibia, and fibula were simultaneously disrupted with open trauma of the left calf. Open reduction and screw-plate fixation of the acetabulum fracture, intramedullary nail fixation of the femoral fracture, and external fixator for his tibia and fibula fracture were carried out.

Conclusion:
The treatment of multiple fractures should focus on life-threatening injuries above all, and then deal with the fractures according to the situation of the patient. Open reduction and internal fixation are priority choices except in some special cases like where soft tissue is in poor condition. Individual therapy and early rehabilitation are effective for homolateral FHI and FKI.
Promotional role of MgO on sorption‐enhanced steam reforming of ethanol over Ni/CaO catalysts
Authors: Sang Sier , Zhao Zhi‐Jian , Tian Hao , Sun Zhao , Li Hongfang , Assabumrungrat Suttichai , Muhammad Tahir , Zeng Liang , Gong Jinlong 
Published: November 2019

AbstractThis article describes the design and synthesis of MgO‐modified Ni/CaO catalysts for sorption‐enhanced steam reforming of ethanol. The results show that the introduction of MgO effectively increases the dispersion of CaO via forming MgCa(CO3)2 precursor. In the prepared MgO‐modified Ni/CaO catalysts, metallic Ni exists around MgO supported on CaO. Both 100% ethanol conversion and >96% hydrogen purity can be stabilized in 10 cycles over the catalyst containing 20 wt% MgO. The interaction between metallic Ni and MgO enhances the sintering resistance of the catalyst. More importantly, reaction pathway studies have confirmed that the formation of CaCO3 hinders the activation of H2O on the Ni/CaO catalyst surface, and thus inhibits the conversion of the reaction intermediates including HCO* and CH
x*. MgO can dissociate H2O to form hydroxyl groups which participate in the conversion of the reaction intermediates, thereby the MgO‐modified Ni/CaO catalysts have better catalytic performance and carbon deposition resistance.
Chronic neutrophilic leukemia: 2020 update on diagnosis, molecular genetics, prognosis, and management
Authors: Szuber Natasha , Elliott Michelle , Tefferi Ayalew 
Published: November 2019

AbstractDisease overviewChronic neutrophilic leukemia (CNL) is a rare, often aggressive myeloproliferative neoplasm (MPN) defined by persistent mature neutrophilic leukocytosis, bone marrow granulocyte hyperplasia, and frequent hepatosplenomegaly. The seminal discovery of oncogenic driver mutations in colony‐stimulating factor 3 receptor (CSF3R) in the majority of patients with CNL in 2013 anchored a new scientific framework, deepening our understanding of its molecular pathogenesis, providing a diagnostic biomarker, and rationalizing the use of pharmacological targeting.Diagnostic criteriaIn 2016, the World Health Organization (WHO) included the presence of activating CSF3R mutations as a central diagnostic feature of CNL. Other criteria include leukocytosis of ≥25 × 109/L comprising >80% neutrophils with <10% circulating precursors and rare blasts, and absence of dysplasia or monocytosis, while not fulfilling criteria for other MPN.Disease updatesIncreasingly comprehensive genetic profiling of CNL has disclosed a complex genomic landscape and additional prognostically relevant mutational combinations. Though prognostic determination and therapeutic decision‐making remain challenging, emerging data on prognostic markers and the use of newer therapeutic agents, such as JAK inhibitors, are helping to define state‐of‐the‐art management in CNL.
Is motor competence associated with the risk of central obesity in preschoolers?
Authors: Henrique Rafael S. , Stodden David F. , Fransen Job , Feitoza Anderson H. P. , Ré Alessandro H. N. , Martins Clarice M. L. , dos Prazeres Thaliane M. P. , Cattuzzo Maria T. 
Published: November 2019

AbstractObjectivesTo investigate the association between motor competence (MC) and central obesity in preschool children.MethodsThe sample comprised of 472 children aged 3 to 5 years (4.58 ± 0.70 years, 248 boys) from Recife, Brazil. MC was assessed using the Test of Gross Motor Development‐2. Waist‐to‐height ratio (WHtR) was calculated and a cutoff of 0.5 was used to define central obesity. Logistic regression was used to examine the association between MC and WHtR ≥ 0.5.ResultsThe prevalence of central obesity (WHtR) was 54.0% and 46.4% for boys and girls, respectively. Older children (OR = 0.61; CI = 0.44‐0.84; P < .01) and those with higher MC in locomotor skills (OR = 0.96; CI = 0.93‐0.99; P < .01) were less likely to present WHtR ≥ 0.5. Sex and object control skills were not associated with WHtR ≥ 0.5.ConclusionsTo reduce the risks of central obesity in children, health practitioners should focus on increasing competence in locomotor skills since preschool years.
Brønsted Acid Catalyzed Tandem Defunctionalization of Biorenewable Ferulic acid and Derivates into Bio‐Catechol
Authors: Bomon Jeroen , Van Den Broeck Elias , Bal Mathias , Liao Yuhe , Sergeyev Sergey , Van Speybroeck Veronique , Sels Bert F. , Maes Bert U. W. 
Published: November 2019

AbstractAn efficient conversion of biorenewable ferulic acid into bio‐catechol has been developed. The transformation comprises two consecutive defunctionalizations of the substrate, that is, C−O (demethylation) and C−C (de‐2‐carboxyvinylation) bond cleavage, occurring in one step. The process only requires heating of ferulic acid with HCl (or H2SO4) as catalyst in pressurized hot water (250 °C, 50 bar N2). The versatility is shown on a variety of other (biorenewable) substrates yielding up to 84 % di‐ (catechol, resorcinol, hydroquinone) and trihydroxybenzenes (pyrogallol, hydroxyquinol), in most cases just requiring simple extraction as work‐up.
Oxidative Stability and Characterization of Quinoa Oil Extracted from Wholemeal and Germ Flours
Authors: Mufari Jesica Romina , Gorostegui Horacio Adrián , Miranda‐Villa Patricia Paola , Bergesse Antonella Estefanía , Calandri Edgardo Luis 
Published: November 2019

AbstractQuinoa seeds are a source of lipids of great quality, and they highlight the content and composition of fatty acids and the presence of antioxidants such as tocopherols. Solvent extraction of quinoa oils was carried out from two matrices (wholemeal and germ flours), and in both cases, the extraction performance, physical–chemical characteristics, and oxidative stability were determined. Oxidative stability of the oil was assessed using an accelerated aging experiment under storage conditions at 60 °C for 12 days, in which the following parameters were measured: peroxide value, acid value, conjugated dienes and trienes, and scavenging radical capacity. Germ flour showed greater extraction yields (27.30 ± 0.15 g/100 g) compared to wholemeal (5.88 ± 0.02 g/100 g). Both oils presented similar physicochemical parameters, although the tocopherol content was higher in the oil extracted from germ flour (1354 vs. 735 mg/kg oil). At the same time, wholemeal oil showed a superior oxidative stability; hence, the wet milled process produces a minor impact on the compounds responsible for protection against lipid oxidation.
Layer‐by‐layer self‐assembly of multifunctional enzymatic UF membranes
Authors: Yurekli Yilmaz 
Published: November 2019

ABSTRACTIn order to eliminate membrane fouling and to ensure enzymatic hydrolysis of urea, multifunctional biocatalytic membranes were prepared by using urease (URE) and trypsin (TRY) enzymes on the sulfonated polysulfone (SPSf) ultrafiltration membrane via layer‐by‐layer (LbL) self‐assembly method. The membrane architecture consisted of multilayer assembly with TRY and URE enzymes as the outer layer and inner sandwiched layer, respectively. Polyethyleneimine (PEI) and alginate (ALG) were used as cationic and anionic polyelectrolytes. Sulfonation and PEI deposition were successfully accomplished as confirmed by attenuated total reflectance Fourier transform infrared spectroscopy, differential scanning calorimetry (DSC) and scanning electron microscopy analysis, contact angle measurements, staining with toluidine blue and Congo red dyes and dead‐end filtration experiments. A characteristic value of SPSf membrane with a high water permeability (1000 L/m2.h.bar) and 95% bovine serum albumin (BSA) rejection was observed. In static conditions, URE activities of SPSf2‐PEI‐URE membrane were not affected by BSA fouling, while TRY immobilizations with increased concentrations (SPSf2‐PEI‐URE‐PEI‐ALG‐TRY) significantly lowered the activity of URE. In dynamic conditions, each deposited layer exhibited individual resistance to flow that can be considered as irreversible fouling and caused 90% of flux decline for the SPSf2‐PEI‐URE‐PEI‐ALG‐TRY membrane assembly. The recovery of the initial flux for the multilayered membrane at the end of six fouling and washing cycles was observed 85%. Moreover, at the end of 5 cycles, 78% of the URE initial activity of the multilayered membrane was preserved. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48750.