bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells
Authors: Tsuneishi Ruri , Saku Noriaki , Miyata Shoko , Javaregowda Palaksha Kanive , Ite Kenta , Toyoda Masashi , Kimura Tohru , Kuroda Masahiko , Nakazawa Atsuko , Kasahara Mureo , Nonaka Hidenori , Kamiya Akihide , Kiyono Tohru , Yamauchi Junji , Umezawa Akihiro 
Published: March 2020

AbstractZone I and zone III hepatocytes metabolize ammonia through urea cycle and drug by cytochrome P450, respectively. Ammonia has a cytotoxic effect, and can therefore be used as a selection agent for enrichment of hepatocytes. Besides, isolated hepatocytes from livers can be propagated ex vivo under appropriate condition. However, it has not been investigated so far whether ammonia-treated hepatocyte-like cells are able to proliferate in vitro. In this study, we employed the ammonia selection strategy to purify hepatocyte-like cells that were differentiated from human pluripotent stem cells (PSCs) that are embryonic stem cells (ESCs) and induced pluripotent stem cells. Hepatocyte-like cells after exposure to ammonia highly expressed the CPS1 gene that metabolizes ammonia to carbamoyl phosphate. The resistance to cytotoxicity or cell death by ammonia is probably attributed to the metabolic activity of ammonia in the cells. In addition to the ammonia metabolism-related genes, ammonia-selected PSC-derived hepatocytes increased expression of the CYP3A4 gene, one of the cytochrome P450 genes, that is mainly expressed in zone III hepatocytes. Ammonia-selected hepatocyte-like cells derived from both ESCs and iPSCs can be propagated in vitro up to 30 population doublings for more than 190 days without affecting expression of the liver-associated genes, implying that the ammonia-selected cells have immortality or equivalent life span on the appropriate feeder cells like ESCs and iPSCs. The long-term cultivation of ammonia-selected hepatocyte-like cells resulted in the increased expression of hepatocyte-associated genes such as the CPS1 and CYP3A4 genes. The ammonia selection method to enrich a hepatocyte population was also applicable to immortalized cells from the liver. Ammonia treatment in combination with in vitro propagation will be used to obtain large amounts of hepatocytes or hepatocyte-like cells for pharmacology, toxicology and regenerative medicine.
Barriers to BIM implementation and ways forward to improve its adoption in the Nigerian AEC firms
Authors: Babatunde Solomon Olusola , Udeaja Chika , Adekunle Adedayo Opeyemi 
Published: March 2020

PurposeBIM has much potential to improve the effectiveness of construction works with respect to design, construction and maintenance. However, many Architecture, Engineering, and Construction (AEC) firms are still lagging in the adoption and implementation of BIM in both developing and developed countries. The purpose of this study is to assess the barriers to BIM implementation, and examine the ways forward to improve BIM adoption within the Nigerian AEC firms.Design/methodology/approachA comprehensive literature review and questionnaire survey were used in the study. The survey targeted four different AEC firms. These include architectural firms, facility management firms, quantity surveying firms and structural engineering firms in Lagos, Nigeria. The data obtained were analyzed using mean score, standard deviation, Kruskal–Wallis test, and factor analysis.FindingsThe study identified 20 barriers to BIM implementation and identified ten ways forward to improve BIM adoption in AEC firms, particularly in Nigeria. The relative importance of both the identified barriers and the ways forward were gauged. The Kruskal–Wallis tests revealed that except for one (out of 20) identified barriers, and one (out of 10) identified ways forward; there is no statistical significant difference in the perceptions of four different AEC firms. The factor analysis result grouped the 20 identified barriers into three major factors to include: weak top management support and BIM environment related issues; cost of BIM software and training issues; and incompatibility, legal, contractual, and culture related issues.Practical implicationsThe significance of the study cannot be over-emphasized due to BIM relevance to construction stakeholders and researchers at large.Originality/valueThe study findings would inform the decisions of the construction stakeholders to make some policy recommendations capable of positively influencing the full BIM implementation in AEC firms.
Identify strategies for cooperatives in East Java with analytic hierarchical process
Authors: Hermawati Adya 
Published: March 2020

Purpose
This study aims to test, analyze, review and describe the problem of “How effective strategies for cooperatives development in East Java.”

Design/methodology/approach
The sample of this study was all cooperative employees in East Java province. The study focuses on the quantitative and qualitative approaches, sampling techniques with purposive sampling on employees of all cooperative districts/cities in East Java. A quantitative approach that is an inferential statistical analysis using the analytic hierarchical process (AHP) method was applied with a diagram/hierarchy.

Findings
The results of this study is the quality of work life criteria, which have an overall weight of 35%, play an important role in experts’ choice of employee performance measurement indicator selection. The development strategy goal for this study is as follows: work behavior (first place), work result (second place) and personal characteristics (third place).

Originality/value
This paper uses the AHP tool to identify strategies for cooperatives in East Java, which are rarely used in previous research, as well as the location of the research (cooperatives in Malang, Indonesia) that has not been studied in depth in other studies.
The application of scam compliance models to investment fraud offending
Authors: Lacey David , Goode Sigi , Pawada Jerry , Gibson Dennis 
Published: March 2020

Purpose
The purpose of this paper is to undertake an exploratory study on mapping the investment fraud methods and tactics used by scammers against the emerging literature on scam compliance.

Design/methodology/approach
Qualitative interviews were conducted with victims of investment fraud supported by the engagement of specialist counsellors and allied health professionals who specialise in scam victim support (including investment fraud).

Findings
Investment fraud offending in the cases sampled exhibited a number of dominant offending traits and methodological themes. These included a strong reliance or dependency on legitimate service provisioning on the part of the fraudster and the use of key trust measures to lure the victim. The empirical data revealed the presence of a number of scam compliance influences captured in the literature, including trust, social influence and urgency, as well as others not previously documented that pave the way for further research attention.

Research limitations/implications
The research only examined a sample of investment fraud victim experiences that engaged a national victim support service immediately following detection over a 24 month period.

Practical implications
The research found that offending relied upon the participation of trust-building signals and measures. Legitimate economy participants appear to play a dominant role in enabling investment scam activities, further creating efficiencies for criminals. The offending tended to follow a number of distinct but connected phases. Impacts were influenced by specific offending attributes, such as whether remote access was given to offenders of a victim’s device, as well as the nature of the identity credentials access.

Originality/value
The research has practically applied an emerging view of scam compliance influences and vulnerabilities within an investment fraud context. The study is novel in its thematic analysis of the distinct phases and tactics used by scammers.
Species temporal persistence promotes the stability of fruit–frugivore interactions across a 5‐year multilayer network
Authors: Costa José M. , Ramos Jaime A. , Timóteo Sérgio , da Silva Luís P. , Ceia Ricardo S. , Heleno Ruben H. 
Published: March 2020

Abstract

Biological communities are intrinsically dynamic, with species and interactions changing over time. However, the temporal dynamics of species interaction networks is usually assessed using independent ‘snapshot’ networks, which may provide an incomplete representation of ecological processes. The use of temporal multilayer networks, where networks (i.e. layers) are formally interconnected via interlayer links, can circumvent such limitation, allowing for a more realistic characterization of community structure and better predictions regarding long‐term processes that rely on species interactions, such as seed dispersal.

We used a 5‐year bird–seed dispersal dataset to explore species and interaction turnover across years, network structure variability and the relationship between species topological roles on each year and their temporal persistence (i.e. species activity). We then implemented a temporal multilayer network approach to compute overall species versatility (centrality) and to reveal the modular structure of the 5‐year multilayer network using changes in species relative abundances as proxies for the interlayer links between time‐sequential networks.

Network topology remained relatively constant across years and interaction turnover was mostly due to true rewiring (new links) between species. Overall, birds, some of which migratory, were temporally more reliable than fleshy‐fruited plants. Interestingly, species present across more years tended to be more important to the structure of each years' seed dispersal network, independently of their relative abundance. The multilayer analysis identified four modules of tightly interacting species, all of which spanning across the 5 years, and with a greater stability in the composition of bird species when compared to plants.

Synthesis. Bird and plant species that are present in more years in the seed dispersal network were also disproportionally important in each year, forming a core of temporally reliable interacting partners for transient species in the network.
Thermal Diversity of Salmon Streams in the Matanuska‐Susitna Basin, Alaska
Authors: Shaftel Rebecca , Mauger Sue , Falke Jeff , Rinella Daniel , Davis Jeff , Jones Leslie 
Published: March 2020

AbstractWe present the first description of summer stream thermal regimes in Alaska using metrics that represent the magnitude, variability, frequency, duration, and timing of temperature events related to salmon life histories. We used cluster analysis to characterize thermal regimes present in the Matanuska‐Susitna (Mat‐Su) Basin based on 10 nonredundant temperature metrics and identified the most important drivers of our thermal regimes using random forests. Our results indicated four distinct thermal regimes among the 248 site‐years in the Mat‐Su Basin. Over 41% of site‐years had cold‐stable temperatures. An additional 22% of site‐years had cold‐variable temperatures and the latest timing of maximum stream temperatures. Twenty‐eight percent of site‐years had warm‐variable temperatures that remained above 13°C for approximately two months. The remaining 9% of site‐years had the warmest and most variable daily maximum temperatures, exceeding 18°C for almost one month, indicating potential impacts to spawning and rearing salmon. Climate and landscape drivers differentiating thermal regimes included spring and summer air temperatures, spring snowpack, summer precipitation, wetlands, and lakes. Climate change projections for 2050–2069 indicated a future shift toward warm thermal regimes and a reduced portfolio of thermal diversity. These results portend negative impacts to some salmon populations and stress the importance of prioritizing actions that maintain thermal regime diversity.
Trends in marijuana use in two Latin American countries: an age, period and cohort study
Authors: Castillo‐Carniglia Alvaro , Rivera‐Aguirre Ariadne , Calvo Esteban , Queirolo Rosario , Keyes Katherine M. , Cerdá Magdalena 
Published: March 2020

AbstractBackground and AimsUruguay and Chile have the highest levels of marijuana use in Latin America, and have experienced consistent increases during the last two decades. We aim to calculate separate age–period–cohort (APC) effects for past‐year marijuana use in Uruguay and Chile, which have similar epidemiologica, and demographic profiles but diverging paths in cannabis regulation.DesignAPC study in which period and cohort effects were estimated as first derivative deviations from their linear age trend, separately by country and gender.SettingUruguay and Chile.ParticipantsGeneral population between 15 and 64 years.MeasurementsPast‐year marijuana use from household surveys with five repeated cross‐sections between 2001 and 2018 in Uruguay (median n = 4616) and 13 between 1994 and 2018 in Chile (median n = 15 895).FindingsMarijuana use prevalence in both countries peaked at 20–24 years of age and increased consistently across calendar years. Period effects were strong and positive, indicating that increases in use were evident across age groups. Relative to 2006 (reference year), Chilean period effects were approximately 48% lower in 1994 and approximately four times higher in 2018; in Uruguay, these effects were approximately 56% lower in 2001 and almost quadrupled in 2018. We observed non‐linear cohort effects in Chile and similar patterns in Uruguay for the overall sample and women. In both countries, marijuana use increased for cohorts born between the mid‐1970s and early 1990s, even in the context of rising period effects. Prevalence was consistently larger for men, but period increases were stronger in women.ConclusionsAge–period–cohort effects on past‐year marijuana use appear to have been similar in Chile and Uruguay, decreasing with age and increasing over time at heterogeneous growth rates depending on gender and cohort. Current levels of marijuana use, including age and gender disparities, seem to be associated with recent common historical events in these two countries.
‘Always take your doctor’s advice’: Does trust moderate the effect of information on inappropriate antibiotic prescribing expectations?
Authors: Thorpe Alistair , Sirota Miroslav , Juanchich Marie , Orbell Sheina 
Published: March 2020

ObjectivesTo reduce overprescribing, health campaigns urge physicians to provide people with information regarding appropriate antibiotic use and encourage the public to trust their physicians’ prescribing decisions. We test (1) whether providing individuals with complete information about the viral aetiology of an illness and the ineffectiveness of antibiotics will reduce inappropriate antibiotic expectations, (2) whether individuals with greater trust in their physician will have lower expectations, and (3) whether individuals with greater trust in their physician will benefit more from the complete information provision and have lower expectations.DesignExperiment 1 features a between‐subjects design (information provision: baseline vs. complete information) with a general measure of participants’ trust in their physician. Experiment 2 features a 2 (physician trustworthiness: low vs. high) × 2 (information provision: baseline vs. complete information) between‐subjects design.MethodsIn Experiment 1, participants (n = 366) reported their trust in their physician, read a vignette describing a hypothetical consultation with a physician for a viral cold and then expressed their expectations for antibiotics. In Experiment 2, participants (n = 380) read a vignette of a consultation with a physician for a viral ear infection then expressed their expectations for antibiotics.ResultsIn both experiments, the provision of complete information significantly reduced inappropriate expectations for antibiotics. Greater trust in physicians was associated with higher antibiotic expectations in Experiment 1, but lower expectations in Experiment 2. In both experiments, trust in physicians appeared to facilitate the effect of information provision, but this effect was weak and inconsistent.ConclusionProviding information about viral aetiology and the ineffectiveness and side effects of antibiotics reduces inappropriate antibiotic expectations. Further research into the effect of trust in physicians as a moderator of the effect information provision is required, particularly given the recent increase in trust‐based antibiotic campaigns.
Statement of contribution

What is already known

Inappropriate expectations for antibiotics encourage overprescribing in primary care.

To reduce inappropriate expectations, interventions often aim to educate people about antibiotics and encourage them to trust their physician.

What does this study add

Causal evidence that clinical information provision reduces but does not eliminate inappropriate antibiotic expectations.

We find that increased trust in physicians is not always associated with lower expectations for antibiotics.

Although increased trust seemed to boost the effect of information provision, this effect was weak and inconsistent.
Derivation of porcine extraembryonic endoderm‐like cells from blastocysts
Authors: Li Yan , Wu Shuang , Yu Yang , Zhang Heng , Wei Renyue , Lv Jiawei , Cai Mingming , Yang Xu , Zhang Yu , Liu Zhonghua 
Published: March 2020

AbstractObjectivesExtraembryonic endoderm (XEN) cells are isolated from primitive endoderm (PrE) of blastocysts. Just like PrE, XEN cells have the ability to differentiate into parietal endoderm (PE) and visceral endoderm (VE), and therefore, they are useful tools for studying mechanisms of PrE cells development and differentiation. Pig is an ideal model for studying human cardiovascular and metabolic diseases and a potential organ source for allotransplantation, while no XEN cell has been obtained from porcine embryos.Materials and MethodsUsing a serum‐free culture system, we directly derived porcine extraembryonic endoderm‐like cells (pXEN‐like cells) from day 6‐7 blastocysts, which could maintain self‐renewal for at least 30 passages.ResultsThe pXEN‐like cells resembled mouse XEN cells with large and flat clone morphology and expressed XEN marker genes but not pluripotent genes. Upon in vitro induction, the cells could differentiate into VE and PE. FGF/MEK signalling was not only essential for the maintenance of pXEN‐like cells, but also the induction of pXEN‐like cells from porcine embryonic stem (pES) cells.ConclusionsWe directly obtained cell lines with XEN characteristics from porcine embryos for the first time. The cells will be helpful tools for studying embryonic development and cell differentiation, which also represent promising cell sources for human regenerative medicine.
Aleukemia cutis: Clinicopathological and molecular investigation of two cases
Authors: Azari‐Yaam Aileen , Safavi Moeinadin , Ghanadan Alireza 
Published: March 2020

AbstractWe describe two cases of acute myeloid leukemia (AML) who presented with cutaneous manifestations. Leukemia cutis (LC) is the cutaneous presentation of any type of leukemia and occurs in 10% to 15% of patients with AML, but cutaneous infiltration of AML rarely precedes the involvement of the bone marrow or peripheral blood and is called “aleukemia cutis.” Our first case presented with facial skin thickening, a manifestation which is known as lionization and his initial clinical diagnosis was nonspecific allergic reaction. Our second case presented with urticaria‐like lesions with the initial clinical and histomorphologic diagnoses of leukocytoclastic vasculitis. Histopathologic examination of skin biopsy specimens in both patients showed diffuse infiltration of the dermis with a monotonous population of intermediate‐sized mononuclear cells by open chromatin and promonocytic features. Bone marrow aspiration leukocyte karyotyping showed normal cytogenetics, and molecular investigation revealed mutations of NPM1 and FLT3 genes. Somatic CEBPA gene mutation was negative in both patients. LC as the first manifestation of leukemia is very rare and could result in delayed diagnosis and affect patient prognosis.
Incretin combination therapy for the treatment of non‐alcoholic steatohepatitis
Authors: Kannt Aimo , Madsen Andreas Nygaard , Kammermeier Claire , Elvert Ralf , Klöckener Tim , Bossart Martin , Haack Torsten , Evers Andreas , Lorenz Katrin , Hennerici Wolfgang , Rocher Corinne , Böcskei Zsolt , Guillemot Jean‐Claude , Mikol Vincent , Pattou Francois , Staels Bart , Wagner Michael 
Published: March 2020

AbstractAimsTo test specific mono‐agonists to the glucagon‐like peptide‐1 receptor (GLP‐1R), glucagon receptor (GCGR) and glucose‐dependent insulinotropic peptide receptor (GIPR), individually and in combination, in a mouse model of diet‐induced non‐alcoholic steatohepatitis (NASH) and fibrosis in order to decipher the contribution of their activities and potential additive effects to improving systemic and hepatic metabolism.Materials and methodsWe induced NASH by pre‐feeding C57BL/6J mice a diet rich in fat, fructose and cholesterol for 36 weeks. This was followed by 8 weeks of treatment with the receptor‐specific agonists 1‐GCG (20 μg/kg twice daily), 2‐GLP1 (3 μg/kg twice daily) or 3‐GIP (30 μg/kg twice daily), or the dual (1 + 2) or triple (1 + 2 + 3) combinations thereof. A dual GLP‐1R/GCGR agonistic peptide, 4‐dual‐GLP1/GCGR (30 μg/kg twice daily), and liraglutide (100 μg/kg twice daily) were included as references.ResultsWhereas low‐dose 1‐GCG or 3‐GIP alone did not influence body weight, liver lipids and histology, their combination with 2‐GLP1 provided additional weight loss, reduction in liver triglycerides and improvement in histological disease activity score. Notably, 4‐dual‐GLP‐1R/GCGR and the triple combination of selective mono‐agonists led to a significantly stronger reduction in the histological non‐alcoholic fatty liver disease activity score compared to high‐dose liraglutide, at the same extent of body weight loss.ConclusionsGCGR and GIPR agonism provide additional, body weight‐independent improvements on top of GLP‐1R agonism in a murine model of manifest NASH with fibrosis.
Differential response among chestnut traditional varieties to the attack of Cydia splendana
Authors: Cuestas Maria Isabel , Martin Maria Angela , Aldebis Hani Kassim , Mena Juan Diego , Martin Luis Miguel , Vargas‐Osuna Enrique 
Published: March 2020

AbstractFruit production of Castanea sativa Miller (Fagaceae) in southern Spain is carried out in traditional orchards using a large number of local varieties. The main areas of production are located in the Natural Park of Sierra de Aracena y Picos de Aroche (Huelva) and the Genal river Valley (Malaga). These agroforestry systems have shown a high genetic diversity and represent an example of in situ conservation. In this region, chestnut mainly suffers from attacks by Cydia splendana (Hübner) (Lepidoptera: Tortricidae), whose larvae feed on the fruits, thereby reducing their quality and economic value. The aim of this study was to evaluate the differential response between the chestnut local varieties to the attack of C. splendana. For that purpose, trees from the most representative varieties were evaluated on various farms. In cases where it was necessary, the trees were identified with a set of eight microsatellite markers. The infestation rate of C. splendana (percentage of infested fruits of the total fruits sampled per tree) differed among varieties, indicating a high genetic component in this effect. The results obtained in this study open the possibility of using this differential response in the control of C. splendana.