bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Immune Activation during Early Gestation or Late Gestation on Inhibitory Markers in Adult Male Rats
Authors: Rahman Tasnim , Weickert Cynthia Shannon , Harms Lauren , Meehan Crystal , Schall Ulrich , Todd Juanita , Hodgson Deborah M. , Michie Patricia T. , Purves-Tyson Tertia 
Published: February 2020

AbstractPeople with schizophrenia exhibit deficits in inhibitory neurons and cognition. The timing of maternal immune activation (MIA) may present distinct schizophrenia-like phenotypes in progeny. We investigated whether early gestation [gestational day (GD) 10] or late gestation (GD19) MIA, via viral mimetic polyI:C, produces deficits in inhibitory neuron indices (GAD1, PVALB, SST, SSTR2 mRNAs) within cortical, striatal, and hippocampal subregions of male adult rat offspring. In situ hybridisation revealed that polyI:C offspring had: (1) SST mRNA reductions in the cingulate cortex and nucleus accumbens shell, regardless of MIA timing; (2) SSTR2 mRNA reductions in the cortex and striatum of GD19, but not GD10, MIA; (3) no alterations in cortical or striatal GAD1 mRNA of polyI:C offspring, but an expected reduction of PVALB mRNA in the infralimbic cortex, and; (4) no alterations in inhibitory markers in hippocampus. Maternal IL-6 response negatively correlated with adult offspring SST mRNA in cortex and striatum, but not hippocampus. These results show lasting inhibitory-related deficits in cortex and striatum in adult offspring from MIA. SST downregulation in specific cortical and striatal subregions, with additional deficits in somatostatin-related signalling through SSTR2, may contribute to some of the adult behavioural changes resulting from MIA and its timing.
RETRACTED ARTICLE: Insomnia, sleepiness, anxiety and depression among different types of gamers in African countries
Authors: Sosso F. A. Etindele , Kuss D. J. , Vandelanotte C. , Jasso-Medrano J. L. , Husain M. E. , Curcio G. , Papadopoulos D. , Aseem A. , Bhati P. , Lopez-Rosales F. , Becerra J. Ramon , D’Aurizio G. , Mansouri H. , Khoury T. , Campbell M. , Toth A. J. 
Published: February 2020

AbstractGaming has increasingly become a part of life in Africa. Currently, no data on gaming disorders or their association with mental disorders exist for African countries. This study for the first time investigated (1) the prevalence of insomnia, excessive daytime sleepiness, anxiety and depression among African gamers, (2) the association between these conditions and gamer types (i.e., non-problematic, engaged, problematic and addicted) and (3) the predictive power of socioeconomic markers (education, age, income, marital status, employment status) on these conditions. 10,566 people from 2 low- (Rwanda, Gabon), 6 lower-middle (Cameroon, Nigeria, Morocco, Tunisia, Senegal, Ivory Coast) and 1 upper-middle income countries (South Africa) completed online questionnaires containing validated measures on insomnia, sleepiness, anxiety, depression and gaming addiction. Results showed our sample of gamers (24 ± 2.8 yrs; 88.64% Male), 30% were addicted, 30% were problematic, 8% were engaged and 32% were non-problematic. Gaming significantly contributed to 86.9% of the variance in insomnia, 82.7% of the variance in daytime sleepiness and 82.3% of the variance in anxiety [p < 0.001]. This study establishes the prevalence of gaming, mood and sleep disorders, in a large African sample. Our results corroborate previous studies, reporting problematic and addicted gamers show poorer health outcomes compared with non-problematic gamers.
Genetic manipulation of the pigment pathway in a sea urchin reveals distinct lineage commitment prior to metamorphosis in the bilateral to radial body plan transition
Authors: Wessel Gary M. , Kiyomoto Masato , Shen Tun-Li , Yajima Mamiko 
Published: February 2020

AbstractEchinoderms display a vast array of pigmentation and patterning in larval and adult life stages. This coloration is thought to be important for immune defense and camouflage. However, neither the cellular nor molecular mechanism that regulates this complex coloration in the adult is known. Here we knocked out three different genes thought to be involved in the pigmentation pathway(s) of larvae and grew the embryos to adulthood. The genes tested were polyketide synthase (PKS), Flavin-dependent monooxygenase family 3 (FMO3) and glial cells missing (GCM). We found that disabling of the PKS gene at fertilization resulted in albinism throughout all life stages and throughout all cells and tissues of this animal, including the immune cells of the coelomocytes. We also learned that FMO3 is an essential modifier of the polyketide. FMO3 activity is essential for larval pigmentation, but in juveniles and adults, loss of FMO3 activity resulted in the animal becoming pastel purple. Linking the LC-MS analysis of this modified pigment to a naturally purple animal suggested a conserved echinochrome profile yielding a pastel purple. We interpret this result as FMO3 modifies the parent polyketide to contribute to the normal brown/green color of the animal, and that in its absence, other biochemical modifications are revealed, perhaps by other members of the large FMO family in this animal. The FMO modularity revealed here may be important in the evolutionary changes between species and for different immune challenges. We also learned that glial cells missing (GCM), a key transcription factor of the endomesoderm gene regulatory network of embryos in the sea urchin, is required for pigmentation throughout the life stages of this sea urchin, but surprisingly, is not essential for larval development, metamorphosis, or maintenance of adulthood. Mosaic knockout of either PKS or GCM revealed spatial lineage commitment in the transition from bilaterality of the larva to a pentaradial body plan of the adult. The cellular lineages identified by pigment presence or absence (wild-type or knock-out lineages, respectively) followed a strict oral/aboral profile. No circumferential segments were seen and instead we observed 10-fold symmetry in the segments of pigment expression. This suggests that the adult lineage commitments in the five outgrowths of the hydropore in the larva are early, complete, fixed, and each bilaterally symmetric. Overall, these results suggest that pigmentation of this animal is genetically determined and dependent on a population of pigment stem cells that are set-aside in a sub-region of each outgrowth of the pentaradial adult rudiment prior to metamorphosis. This study reveals the complex chemistry of pigment applicable to many organisms, and further, provides an insight into the key transitions from bilateral to pentaradial body plans unique to echinoderms.
Quantum-inspired cascaded discrete-time quantum walks with induced chaotic dynamics and cryptographic applications
Authors: Abd El-Latif Ahmed A. , Abd-El-Atty Bassem , Amin Mohamed , Iliyasu Abdullah M. 
Published: February 2020

AbstractDesigning efficient and secure cryptosystems has been a preoccupation for many scientists and engineers for a long time wherein they use chaotic systems to design new cryptosystems. While one dimensional (1-D) chaotic maps possess powerful properties compared to higher dimension ones, they are vulnerable to various attacks due to their small key space, chaotic discontinuous ranges, and degradation in chaotic dynamical behaviours. Moreover, when simulated on a computer, every such chaotic system produces a periodic cycle. Meanwhile, quantum random walks exhibit the potential for deployment in efficient cryptosystem design, which makes it an excellent solution for this problem. In this context, we present a new method for constructing substitution boxes (S-boxes) based on cascaded quantum-inspired quantum walks and chaos inducement. The performance of the proposed S-box scheme is investigated via established S-box evaluation criterion and outcomes suggest that the constructed S-box has significant qualities for viable applications information security. Further, we present an efficient scheme for pseudo-random numbers generation (PRNG) whose sustainability over long periods remedies the periodicity problem associated with traditional cryptographic applications. Furthermore, by combining the two mechanisms, an atypical image encryption scheme is introduced. Simulation results and analysis validate that the proposed image encryption algorithm will offer gains in many cryptographic applications.
Domestication leads to increased predation susceptibility
Authors: Solberg Monica F. , Robertsen Grethe , Sundt-Hansen Line E. , Hindar Kjetil , Glover Kevin A. 
Published: February 2020

AbstractDomestication involves adapting animals to the human-controlled environment. Genetic changes occurring during the domestication process may manifest themselves in phenotypes that render domesticated animals maladaptive for life in the wild. Domesticated Atlantic salmon frequently interbreed with wild conspecifics, and their offspring display reduced survival in the wild. However, the mechanism(s) contributing to their lower survival in the wild remains a subject of conjecture. Here, we document higher susceptibility to predation by brown trout in fast-growing domesticated salmon, as compared to their slow-growing wild conspecifics, demonstrating that directional selection for increased growth comes at a cost of decreased survival when under the risk of predation, as predicted by the growth/predation risk trade-off. Despite earlier documentation of altered risk-taking behavior, this study demonstrates for the first time that domestication of Atlantic salmon has lead to increased predation susceptibility, and that this consitutes a mechanism underpinning the observed survial differences in the wild.
Seasonal to Inter-Annual Variability of Primary Production in Chesapeake Bay: Prospects to Reverse Eutrophication and Change Trophic Classification
Authors: Harding Lawrence W. , Mallonee Michael E. , Perry Elgin S. , David Miller W. , Adolf Jason E. , Gallegos Charles L. , Paerl Hans W. 
Published: February 2020

AbstractEstuarine-coastal ecosystems are rich areas of the global ocean with elevated rates of organic matter production supporting major fisheries. Net and gross primary production (NPP, GPP) are essential properties of these ecosystems, characterized by high spatial, seasonal, and inter-annual variability associated with climatic effects on hydrology. Over 20 years ago, Nixon defined the trophic classification of marine ecosystems based on annual phytoplankton primary production (APPP), with categories ranging from “oligotrophic” to “hypertrophic”. Source data consisting of shipboard measurements of NPP and GPP from 1982 to 2004 for Chesapeake Bay in the mid-Atlantic region of the United States supported estimates of APPP from 300 to 500 g C m−2 yr−1, corresponding to “eutrophic” to “hypertrophic” categories. Here, we developed generalized additive models (GAM) to interpolate the limited spatio-temporal resolution of source data. Principal goals were: (1) to develop predictive models of NPP and GPP calibrated to source data (1982 to 2004); (2) to apply the models to historical (1960s, 1970s) and monitoring (1985 to 2015) data with adjustments for nutrient loadings and climatic effects; (3) to estimate APPP from model predictions of NPP; (4) to test effects of simulated reductions of phytoplankton biomass or nutrient loadings on trophic classification based on APPP. Simulated 40% decreases of euphotic-layer chl-a or TN and NO2 + NO3 loadings led to decreasing APPP sufficient to change trophic classification from “eutrophic’ to “mesotrophic” for oligohaline (OH) and polyhaline (PH) salinity zones, and from “hypertrophic” to “eutrophic” for the mesohaline (MH) salinity zone of the bay. These findings show that improved water quality is attainable with sustained reversal of nutrient over-enrichment sufficient to decrease phytoplankton biomass and APPP.
Active Cloaking of a Non-Uniform Scatterer
Authors: Ang Paris , Eleftheriades George V. 
Published: February 2020

AbstractAn object illuminated by an electromagnetic wave can be actively cloaked using a surface conformal array of radiating sources to cancel out scattering. This method is promising as elementary antennas can be used as sources while its active nature can surpass passivity-based performance limitations. While this technique has been conceptually extended to accommodate complex geometries, experimental validation past simple uniform scatterers is lacking. To address this scarcity, the design and experimental demonstration of a low-profile, active cloak capable of concealing a complex, metallic, polygonal target is presented. This cloak is constructed with commercially available monopoles and enclosed within a parallel-plate waveguide-based apparatus to approximate a quasi-2D environment. Performance is then assessed when the target is illuminated at either frontal or oblique incidence by a 1.2 GHz cylindrical wave. Overall, the cloak reduces the target’s scattering cross-section by an average of 7.2 dB at frontal incidence and 8.6 dB at oblique incidence. These results demonstrate the feasibility of this kind of active cloaking for more complex scatterers containing flat surfaces and edges. Further analysis shows that the cloak possesses a functional bandwidth of 14% and can be reconfigured for single frequency operation over 0.8–1.8 GHz.
Co-production of DHA and squalene by thraustochytrid from forest biomass
Authors: Patel Alok , Liefeldt Stephan , Rova Ulrika , Christakopoulos Paul , Matsakas Leonidas 
Published: February 2020

AbstractOmega-3 fatty acids, and specifically docosahexaenoic acid (DHA), are important and essential nutrients for human health. Thraustochytrids are recognised as commercial strains for nutraceuticals production, they are group of marine oleaginous microorganisms capable of co-synthesis of DHA and other valuable carotenoids in their cellular compartment. The present study sought to optimize DHA and squalene production by the thraustochytrid Schizochytrium limacinum SR21. The highest biomass yield (0.46 g/gsubstrate) and lipid productivity (0.239 g/gsubstrate) were observed with 60 g/L of glucose, following cultivation in a bioreactor, with the DHA content to be 67.76% w/wtotal lipids. To reduce costs, cheaper feedstocks and simultaneous production of various value-added products for pharmaceutical or energy use should be attempted. To this end, we replaced pure glucose with organosolv-pretreated spruce hydrolysate and assessed the simultaneous production of DHA and squalene from S. limacinum SR21. After the 72 h of cultivation period in bioreactor, the maximum DHA content was observed to 66.72% w/wtotal lipids that was corresponded to 10.15 g/L of DHA concentration. While the highest DHA productivity was 3.38 ± 0.27 g/L/d and squalene reached a total of 933.72 ± 6.53 mg/L (16.34 ± 1.81 mg/gCDW). In summary, we show that the co-production of DHA and squalene makes S. limacinum SR21 appropriate strain for commercial-scale production of nutraceuticals.
Characterising the spatial distribution of opportunities and constraints for land sparing in Brazil
Authors: dos Santos Juliana Silveira , Feltran-Barbieri Rafael , Fonte Ellen S. , Balmford Andrew , Maioli Veronica , Latawiec Agnieszka , Strassburg Bernardo B. N. , Phalan Benjamin T. 
Published: February 2020

AbstractBrazil is a megadiversity country with more tropical forest than any other, and is a leading agricultural producer. The technical potential to reconcile these roles by concentrating agriculture on existing farmland and sparing land for nature is well-established, but the spatial overlap of this potential with conservation priorities and institutional constraints remains poorly understood. We mapped conservation priorities, food production potential and socio-economic variables likely to influence the success of land sparing. Pasture occupies 70% of agricultural land but contributes ≤11% of the domestic food supply. Increasing yields on pasture would add little to Brazil’s food supply but – if combined with concerted conservation and restoration policies – provides the greatest opportunities for reducing land demand. Our study illustrates a method for identifying municipalities where land-sparing policies are most likely to succeed, and those where further effort is needed to overcome constraints such as land tenure insecurity, lack of access to technical advice, labour constraints, and non-compliance with environmental law.
Renoprotective effects of Gushen Jiedu capsule on diabetic nephropathy in rats
Authors: Zhang Lei , Yang Zhirui , Zhao Yidan , Yang Xinyu , Meng Xintong , Liu Juan , Liu Yi , Yan Can , Yan Dan 
Published: February 2020

AbstractGushen Jiedu capsule (GSJD) is a formula that has been widely used in traditional Chinese medicine for the prevention and treatment of diabetic nephropathy (DN). However, the mechanism underlying the protective effects of GSJD on DN is still unclear. This study was performed to clarify the therapeutic effects of GSJD on DN and its underlying mechanisms. High-fat diet- and streptozotocin-induced DN rats were treated with or without GSJD suspension by gavage for 8 weeks, and biochemical changes in blood and urine were analysed. Kidneys were isolated for histological, TUNEL and Western blot analysis. Compared to the DN group, the GSJD-treated groups exhibited decreased urinary albumin, ameliorated renal dysfunction, including serum creatinine and blood urea nitrogen, and attenuated total cholesterol, triglyceride and total protein levels. However, there were no significant effects of GSJD on body weight, fasting blood glucose or albuminuria. Histology showed that GSJD could retard the progression of DN and decrease the apoptosis rate from 52% to less than 20%. Western blot analysis showed that GSJD could regulate the mitochondrial apoptotic pathway by downregulating the expression of Bax and upregulating the expression of BCL-2 in the kidneys of DN rats. Moreover, the Akt pathway, an upstream signalling pathway of the BCL-2 family, was also ameliorated by GSJD. Further, the podocyte foot process markers podocin and nephrin were upregulated by GSJD in DN rats. This study demonstrated that GSJD might play a renoprotective role by inhibiting apoptosis and regulating the mitochondrial apoptotic and Akt pathways during pathological changes in DN.
Investigation of DNA variants specific to ROBO2 Isoform ‘a’ in Irish vesicoureteric reflux patients reveals marked CpG island variation
Authors: Darlow John M. , Dobson Mark G. , Green Andrew J. , Puri Prem , Barton David E. 
Published: February 2020

AbstractROBO2 gene disruption causes vesicoureteric reflux (VUR) amongst other congenital anomalies. Several VUR patient cohorts have been screened for variants in the ubiquitously expressed transcript, ROBO2b, but, apart from low levels in a few adult tissues, ROBO2a expression is confined to the embryo, and might be more relevant to VUR, a developmental disorder. ROBO2a has an alternative promoter and two alternative exons which replace the first exon of ROBO2b. We screened probands from 251 Irish VUR families for DNA variants in these. The CpG island of ROBO2a, which includes the non-coding first exon, was found to contain a run of six variants abolishing/creating CpG dinucleotides, including a novel variant, present in the VUR cases in one family, that was not present in 592 healthy Irish controls. In three of these positions, the CpG was created by the non-reference allele, and the reference allele was not the nucleotide that would result from spontaneous deamination of methylcytosine to thymine, suggesting that there might have been selection for variability in number of CpGs in this island. This is in marked contrast to the CpG island at the start of ROBO2b, which only contained a single variant that abolishes a CpG.
Holographic molecular binding assays
Authors: Zagzag Yvonne , Soddu M. Francesca , Hollingsworth Andrew D. , Grier David G. 
Published: February 2020

AbstractWe demonstrate that holographic particle characterization can directly detect binding of proteins to functionalized colloidal probe particles by monitoring the associated change in the particles’ size. This label-free molecular binding assay uses in-line holographic video microscopy to measure the diameter and refractive index of individual probe spheres as they flow down a microfluidic channel. Pooling measurements on 104 particles yields the population-average diameter with an uncertainty smaller than 0.5 nm, which is sufficient to detect sub-monolayer coverage by bound proteins. We demonstrate this method by monitoring binding of NeutrAvidin to biotinylated spheres and binding of immunoglobulin G to spheres functionalized with protein A.
Selective interaction between phytomediated anionic silver nanoparticles and mercury leading to amalgam formation enables highly sensitive, colorimetric and memristor-based detection of mercury
Authors: Sangaonkar Geetanjali M. , Desai Megha P. , Dongale Tukaram D. , Pawar Kiran D. 
Published: February 2020

AbstractPresently, nanotechnology is being foreseen to play an important role in developing analytical assays for the detection of pollutants like mercury (Hg2+). In this study, Kokum fruit mediated silver nanoparticles (AgNPs) were differentially centrifuged to prepare anionic, monodispersed AgNPs to develop a highly sensitive, colorimetric and memristor-based assay for detection of Hg2+ in water samples. The investigation of the highly selective reaction between AgNPs and Hg2+ using HAADF-STEM images and EDS spectrum indicated the amalgam formation through etching and under potential deposition which resulted in a visible color change from brown to colorless, change in SPR intensity and also change in memristive switching like property of AgNPs. The developed colorimetric assay detected Hg2+ with a limit of detection (LOD) of 6.2 ppb and limit of quantification (LOQ) of 18.9 ppb and, quantitatively recovered Hg2+ with good accuracy and precision (RSD < 2%). Further, the test of memristive switching like property of AgNPs demonstrated frequency-dependent shrinkage of I–V hysteresis loop indicating memristive switching like property. The test of the sensitivity of Hg2+ detection was estimated to be 8.7 ppb as the LOD and 26.4 ppb as LOQ. Like the colorimetric assay, the memristor-based assay also recovered Hg2+ with good accuracy and precision.
High-density neutrophils in MGUS and multiple myeloma are dysfunctional and immune-suppressive due to increased STAT3 downstream signaling
Authors: Romano A. , Parrinello N. L. , Simeon V. , Puglisi F. , La Cava P. , Bellofiore C. , Giallongo C. , Camiolo G. , D’Auria F. , Grieco V. , Larocca F. , Barbato A. , Cambria D. , La Spina E. , Tibullo D. , Palumbo G. A. , Conticello C. , Musto P. , Di Raimondo F. 
Published: February 2020

AbstractTo understand neutrophil impairment in the progression from MGUS through active MM, we investigated the function of mature, high-density neutrophils (HDNs), isolated from peripheral blood. In 7 MM, 3 MGUS and 3 healthy subjects by gene expression profile, we identified a total of 551 upregulated and 343 downregulated genes in MM-HDN, involved in chemokine signaling pathway and FC-gamma receptor mediated phagocytosis conveying in the activation of STAT proteins. In a series of 60 newly diagnosed MM and 30 MGUS patients, by flow-cytometry we found that HDN from MM, and to a lesser extend MGUS, had an up-regulation of the inducible FcγRI (also known as CD64) and a down-regulation of the constitutive FcγRIIIa (also known as CD16) together with a reduced phagocytic activity and oxidative burst, associated to increased immune-suppression that could be reverted by arginase inhibitors in co-culture with lymphocytes. In 43 consecutive newly-diagnosed MM patients, who received first-line treatment based on bortezomib, thalidomide and dexamethasone, high CD64 could identify at diagnosis patients with inferior median overall survival (39.5 versus 86.7 months, p = 0.04). Thus, HDNs are significantly different among healthy, MGUS and MM subjects. In both MGUS and MM neutrophils may play a role in supporting both the increased susceptibility to infection and the immunological dysfunction that leads to tumor progression.