bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dearomatizing [4+1] Spiroannulation of Naphthols: Discovery of Thermally Activated Delayed Fluorescent Materials
Authors: Liang Wenbo , Yang Yudong , Yang Mufan , Zhang Min , Li Chengming , Ran You , Lan Jingbo , Bin Zhengyang , You Jingsong 
Published: October 2020

AbstractDisclosed here is a palladium‐catalyzed direct [4+1] spiroannulation of ortho‐C−H bonds of naphthols with cyclic diaryliodonium salts to construct spirofluorenyl naphthalenones (SFNP) under mild reaction conditions. This spiroannulation directly transforms the hydroxy group into a carbonyl group, and also tolerates reactive functional groups such as the halo groups, which provide an opportunity to rapidly assemble structurally new thermally activated delayed fluorescent (TADF) materials that feature a carbonyl group with an adjacent spirofluorenyl unit as the acceptor. As an illustrated example, the OLED device utilizing the assembled DMAC‐SFNP as the host material exhibits a low turn‐on voltage of 2.5 V and an ultra‐high external quantum efficiency of 32.2 %. This work provides inspiration for structurally new TADF materials, and also displays the potential of C−H activation as a synthetic strategy for the innovation of optoelectronic materials.
Photoinduced Dearomatizing Three‐Component Coupling of Arylphosphines, Alkenes, and Water
Authors: Masuda Yusuke , Tsuda Hiromu , Murakami Masahiro 
Published: October 2020

AbstractA unique photoinduced reaction that couples a triarylphosphine, an alkene, and water to produce 2‐(cyclohexa‐2,5‐dienyl)ethylphosphine oxide is reported herein. The alkene inserts into a C(aryl)−P bond of the arylphosphine, the aryl ring is dearomatized into the cyclohexadienyl ring, and the phosphorus is oxidized. The three components are all readily available, and their intermolecular coupling significantly increases molecular complexity. The products formed are applicable to the Wittig olefination.
Delivery of a Masked Uranium(II) by an Oxide‐Bridged Diuranium(III) Complex
Authors: Modder Dieuwertje K. , Palumbo Chad T. , Douair Iskander , Fadaei‐Tirani Farzaneh , Maron Laurent , Mazzanti Marinella 
Published: October 2020

AbstractOxide is an attractive linker for building polymetallic complexes that provide molecular models for metal oxide activity, but studies of these systems are limited to metals in high oxidation states. Herein, we synthesized and characterized the molecular and electronic structure of diuranium bridged UIII/UIV and UIII/UIII complexes. Reactivity studies of these complexes revealed that the U−O bond is easily broken upon addition of N‐heterocycles resulting in the delivery of a formal equivalent of UIII and UII, respectively, along with the uranium(IV) terminal‐oxo coproduct. In particular, the UIII/UIII oxide complex effects the reductive coupling of pyridine and two‐electron reduction of 4,4′‐bipyridine affording unique examples of diuranium(III) complexes bridged by N‐heterocyclic redox‐active ligands. These results provide insight into the chemistry of low oxidation state metal oxides and demonstrate the use of oxo‐bridged UIII/UIII complexes as a strategy to explore UII reactivity.
Trend in creatinine determining methods: Conventional methods to molecular‐based methods
Authors: Narimani Ramin , Esmaeili Mahdad , Rasta Seyed Hossein , Khosroshahi Hamid Tayebi , Mobed Ahmad 
Published: October 2020

AbstractRenal failure (RF) disease is ranked as one of the most prevalent diseases with severe morbidity and mortality. Early diagnosis of RF leads to subsequent control of disease to reduce the poor prognosis. The level of sera creatinine is considered as a significant biomarker for kidney biofunction, which is routinely detected by the Jaffe reaction. The normal range for creatinine in the blood may be 0.84‐1.21 mg/dL. Low accuracy, insufficient sensitivity, explosive and toxicity of picric acid, and pseudo‐interaction with nonspecific elements such as ammonium ions in the Jaffe method lead to the development of various techniques for precise detection of creatinine such as spectroscopic, electrochemical, and chromatography approaches and sensors based on enzymes, molecular imprinted polymer and nanoparticles, etc. Based on previously established results, they are trying to construct sensors with high accuracy, optimum sensitivity, acceptable linear/calibration range, and limit of detection, which are small in size and applicable by the patient him/herself (point‐of‐care testing). By comparing the results of research, a molecularly imprinted electrochemiluminescence‐based sensor with linear/calibration range of 5‐1 mMconcentration of creatinine and the detection limit of 0.5 nM has the best detectable resolution with 2 million measurable points. In this paper, we will review the recently developed methods for measuring creatinine concentration and renal biofunction.
Synthesis and luminescence spectroscopy study of a novel orange‐red colour emissions phosphor based on Tb3+ ion‐doped Na2ZnP2O7
Authors: Belbal Rim , Gacem Lakhdar , Bentria Bachir , Ait Ahsaine Hassan , Soltani Mohamed Toufik , Saidat Boubakeur , Ghezal Elhadj Ahmed , Gueddim Ahmed , Guerbous Lakhdar 
Published: October 2020

AbstractHere, we report the synthesis and study of new orange‐red (OR) colour‐emitting phosphors Na2ZnP2O7:xTb3+ (x = 1, 1.25, 1.5, and 1.75 mol%) using a conventional solid‐state reaction. The sample crystallographic structures and their room temperature photoluminescence properties were measured using X‐ray powder diffraction, excitation and emission spectra, as well as emission decay curves. X‐ray diffraction analysis showed an isostructural tetragonal structure for all the doped materials with no impurities. Several colour emissions corresponding to 5D4→7F6 (blue), 5D4→7F5 (green), 5D4→7F4 (orange) and 5D4→7F3,2,0 (red) intraconfigurational transitions were observed and investigated. These colours were dominated by the OR colours. 5D4 energy level lifetime was independent of Tb3+ concentration. Various radiative and luminescence parameters, such as experimental branching ratio, radiative decay rate, and luminescence quantum efficiencies, were calculated and discussed. Chromaticity diagram calculations illustrated an orange emission for all the studied materials.
Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates
Authors: Mezzina Mariela P. , Manoli María Tsampika , Prieto M. Auxiliadora , Nikel Pablo I. 
Published: October 2020

AbstractGrowing environmental concern sparks renewed interest in the sustainable production of (bio)materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are isotactic polymers that play a critical role in the central metabolism of producer bacteria, as they act as dynamic reservoirs of carbon and reducing equivalents. PHAs continue to attract industrial attention as a starting point toward renewable, biodegradable, biocompatible, and versatile thermoplastic and elastomeric materials. Pseudomonas species have been known for long as efficient biopolymer producers, especially for medium‐chain‐length PHAs. The surge of synthetic biology and metabolic engineering approaches in recent years offers the possibility of exploiting the untapped potential of Pseudomonas cell factories for the production of tailored PHAs. In this article, an overview of the metabolic and regulatory circuits that rule PHA accumulation in Pseudomonas putida is provided, and approaches leading to the biosynthesis of novel polymers (e.g., PHAs including nonbiological chemical elements in their structures) are discussed. The potential of novel PHAs to disrupt existing and future market segments is closer to realization than ever before. The review is concluded by pinpointing challenges that currently hinder the wide adoption of bio‐based PHAs, and strategies toward programmable polymer biosynthesis from alternative substrates in engineered P. putida strains are proposed.
Association between GPx‐1 polymorphisms and personality traits in healthy Chinese‐Han subjects
Authors: Shao Xiaojun , Sun Dongxue , Zhang Bihui , Cheng Lingfei , Yan Ci , Zhu Gang 
Published: October 2020

AbstractObjectivesCloninger developed the three‐dimensional personality theory and Tridimensional Personality Questionnaire (TPQ), which shows that some dimensions of personality traits are heritable and related to neurotransmitters including dopamine. glutathione peroxidase 1 (GPx1) plays an important role in metabolic dopamine change and closely relates to neurological and psychiatric disorders. The impact of GPx‐1 polymorphisms has been rarely explored in the field of personality traits. We decide to explore the relationships between them in healthy Chinese‐Han subjects by using the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP).MethodsIn our study, 493 healthy Chinese‐Han participants (male = 234, female = 259) were recruited. 2 ml of EDTA‐treated blood from each volunteer was taken; meanwhile, personality traits were assessed by TPQ. We detected the genotypes of selected two polymorphisms through PCR‐RFLP after extracting DNA. Finally, the association between different genotypes and TPQ scores was performed using SPSS, p < .05 is seen as significant statistical significance.ResultsOur data found a correlation between rs1800668 and novelty seeking (NS) subscale NS2 (X2 = 7.392, p = .025). While the results showed the rs1050450 was significantly associated with NS4 (X2 = 6.059, p = .048). Regarding sex stratification, there was a significant difference in the NS2 score (X2 = 8.232, p = .016) among women for rs1800668. No sex effect was observed for either genotype for rs1050450.ConclusionGPx‐1polymorphism is related to personality traits in healthy Chinese‐Han subjects. Our results suggested that GPx‐1 may be involved in the biological mechanisms and be a potential gene that influenced personality.
Sonodynamic therapy reduces iron retention of hemorrhagic plaque
Authors: Li Bicheng , Gong Jie , Sheng Siqi , Lu Minqiao , Guo Shuyuan , Yao Jianting , Zhang Haiyu , Zhao Xuezhu , Cao Zhengyu , Sun Xin , Wang Huan , Cao Yang , Jiang Yongxing , Tian Zhen , Liu Bin , Zhao Hua , Zhang Zhiguo , Jin Hong , Tian Ye 
Published: October 2020

AbstractIntraplaque hemorrhage (IPH) plays a major role in the aggressive progression of vulnerable plaque, leading to acute cardiovascular events. We previously demonstrated that sonodynamic therapy (SDT) inhibits atherosclerotic plaque progression. In this study, we investigated whether SDT could also be applied to treat more advanced hemorrhagic plaque and addressed the underlying mechanism. SDT decreased atherosclerotic burden, positively altered atherosclerotic lesion composition, and alleviated iron retention in rabbit hemorrhagic plaques. Furthermore, SDT reduced iron retention by stimulating ferroportin 1 (Fpn1) expression in apolipoprotein E (ApoE)−/− mouse plaques with high susceptibility to IPH. Subsequently, SDT inhibited iron‐overload‐induced foam‐cell formation and pro‐inflammatory cytokines secretion in vitro. Moreover, SDT reduced levels of the labile iron pool and ferritin expression via the reactive oxygen species (ROS)‐nuclear factor erythroid 2‐related factor 2 (Nrf2)‐FPN1 pathway. SDT exerted therapeutic effects on hemorrhagic plaques and reduced iron retention via the ROS‐Nrf2‐FPN1 pathway in macrophages, thereby suggesting that it is a potential translational strategy for patients with advanced atherosclerosis in clinical practice.
Effect of an alkyl spacer on the morphology and internalization of MUC1 aptamer‐naphthalimide amphiphiles for targeting and imaging triple negative breast cancer cells
Authors: Kuang Huihui , Schneiderman Zachary , Shabana Ahmed M. , Russo Gabriella C. , Guo Jun , Wirtz Denis , Kokkoli Efrosini 
Published: October 2020

AbstractDespite decades of research, there are few targeted treatment options available for triple negative breast cancer (TNBC), leaving chemotherapy, and radiation treatment regimes with poor response and high toxicity. Herein aptamer‐amphiphiles were synthesized which selectively bind to the mucin‐1 (MUC1) glycoprotein that is overexpressed in TNBC cells. These amphiphiles have a fluorescent tail (1,8‐naphthalimide or 4‐nitro‐1,8‐naphthalimide) which enables self‐assembly of the amphiphiles and allows for easy visualization without the requirement for further conjugation of a fluorophore. Interestingly, the length of the alkyl spacer (C4 or C12) between the aptamer and tail was shown to influence the morphology of the self‐assembled structure, and thus its ability to internalize into the TNBC cells. While both the MUC1 aptamer‐C4‐napthalimide spherical micelles and the MUC1 aptamer‐C12‐napthalimide long cylindrical micelles showed internalization into MDA‐MB‐468 TNBC cells but not the noncancerous MCF‐10A breast cells, the cylindrical micelles showed greatly enhanced internalization into the MDA‐MB‐468 cells. Similar patterns of enhanced binding and internalization were observed between the MUC1 aptamer‐C12‐napthalimide cylindrical micelles and SUM159 and MDA‐MB‐231 TNBC cells. The MUC1 aptamer cylindrical micelles were not toxic to the cells, and when used to deliver doxorubicin to the TNBC cells, were shown to be as cytotoxic as free doxorubicin. Moreover, a pharmacokinetic study in mice showed a prolonged systemic circulation time of the MUC1 aptamer cylindrical micelles. There was a 4.6‐fold increase in the elimination half‐life of the aptamer cylindrical micelles, and their clearance decreased 10‐fold compared to the MUC1 aptamer spherical micelles. Thus, the MUC1 aptamer‐C12‐napthalimide nanofibers represent a promising vehicle that could be used for easy visualization and targeted delivery of therapeutic loads to TNBC cells.
Repressor‐Like On‐Off Regulation of Protein Expression by the DNA‐Binding Transcription Activator‐Like Effector in T7 Promoter‐Based Cell‐Free Protein Synthesis
Authors: Sakono Masafumi , Hayakawa Ryoto 
Published: October 2020

AbstractThe aim of this study was to develop a transcription activator‐like effector (TALE)‐based technology to regulate protein synthesis in cell‐free systems. We attempted to regulate the T7 promoter system, which has no natural mechanism of expression control, and sought to arbitrarily induce protein expression through the formation and dissociation of TALE and target DNA complexes. Protein synthesis was performed in a cell‐free system in the presence of TALE, which recognized and bound to a sequence upstream of the T7 promoter, and protein expression was suppressed by approximately 80 % compared to in the absence of TALE. This suggests that masking part of the promoter region strongly suppresses protein synthesis. Additionally, competitive inhibition of TALE binding to the target DNA template led to protein synthesis levels that were equivalent to the levels in the absence of TALE. Our results demonstrate that DNA recognition by TALE can regulate the expression of the T7 promoter system.
Heterologous Expression of an Unusual Ketosynthase, SxtA, Leads to Production of Saxitoxin Intermediates in Escherichia coli
Authors: Soeriyadi Angela H. , Mazmouz Rabia , Pickford Russell , Al‐Sinawi Bakir , Kellmann Ralf , Pearson Leanne A. , Neilan Brett A. 
Published: October 2020

AbstractParalytic shellfish toxins (PSTs) are neurotoxic alkaloids produced by freshwater cyanobacteria and marine dinoflagellates. Due to their antagonism of voltage‐gated sodium channels in excitable cells, certain analogues are of significant pharmacological interest. The biosynthesis of the parent compound, saxitoxin, is initiated with the formation of 4‐amino‐3‐oxo‐guanidinoheptane (ethyl ketone) by an unusual polyketide synthase‐like enzyme, SxtA. We have heterologously expressed SxtA from Raphidiopsis raciborskii T3 in Escherichia coli and analysed its activity in vivo. Ethyl ketone and a truncated analogue, methyl ketone, were detected by HPLC‐ESI‐HRMS analysis, thus suggesting that SxtA has relaxed substrate specificity in vivo. The chemical structures of these products were further verified by tandem mass spectrometry and labelled‐precursor feeding with [guanidino‐15N2] arginine and [1,2‐13C2] acetate. These results indicate that the reactions catalysed by SxtA could give rise to multiple PST variants, including analogues of ecological and pharmacological significance.
Another nail in the coffin for the use of risk‐adjusted mortality after percutaneous coronary intervention as a quality indicator
Authors: Grines Cindy L. , Tummala Pradyumna 
Published: October 2020

Key Points
Use of mortality is a poor indicator of quality for percutaneous coronary intervention (PCI).
Target vessel revascularization has similar limitations but may be superior to mortality as a quality indicator.
We propose increased use of intravascular imaging to improve PCI outcomes.
Use and outcomes of the PK Papyrus covered stent in France: SOS PK Papyrus Registry
Authors: Hernández‐Enríquez Marco , Belle Loic , Madiot Hende , Pansieri Michel , Souteyrand Geraud , de Poli Fabiel , Piot Christophe , Boueri Ziad , Gerbaud Edouard , Boiffard Emmanuel , Benamer Hakim , Lattuca Benoit , Commeau Philippe , Gervasoni Richard , Rangé Gregoire , Lhoest Nicolas , Marliere Stéphanie , Abdellaoui Mohamed , Delarche Nicolas , Zemour Gilles , Armengaud Jean , Carre Max , Levesque Sebastien , Boudou Nicolas 
Published: October 2020

AbstractObjectivesTo evaluate the rate of procedural success and long‐term outcomes of the PK Papyrus (PKP) covered stent (CS).BackgroundCS are essential in the treatment of coronary artery perforation (CAP). They have also been used to treat coronary artery aneurysms. Limited evidence is available on clinical outcomes with the PKP.MethodsThis was a multicenter, observational, retrospective, and prospective study. Consecutive patients undergoing intentional PKP implantation in 22 centers in France were included. The primary endpoint was the rate of procedural success. Secondary endpoints included rates of death, myocardial infarction (MI), target lesion revascularization (TLR), in‐stent restenosis (ISR), and stent thrombosis (ST).ResultsData from 130 patients were analyzed (mean age 72.5 ± 10.5 years; 71% men). The main indication for PKP was CAP, in 84 patients (65%). Delivery success was achieved in 95% and procedural success in 91%. During the in‐hospital stay, 15 patients died (12%) and 7 (5%) presented with ST. Data from 127 patients were available at 19.2 ± 12.8 month follow‐up. Thirty‐three patients died (26%), 15 (12%) had an MI and 21 (17%) presented with TLR. TLR was due to ISR in 12 patients (9%), 10 had definite ST (8%) and 1 patient for stent under‐expansion.ConclusionsThe principal indication for PKP was CAP. PKP had high rates of delivery and procedural success. At long‐term follow‐up, there was a high rate of TLR, mainly due to ISR and ST. These results are consistent with previously reported data in these clinical settings.
The predictive value of CHADS2 score for subclinical cerebral ischemia after carotid artery stenting (from the PREVENT‐CAS trial)
Authors: Çelik Ömer , Güner Ahmet , Kalçık Macit , Güler Arda , Demir Ali Rıza , Demir Yusuf , Uygur Begum , Şahin Ahmet Anıl , Topel Çağdaş , Ertürk Mehmet 
Published: October 2020

AbstarctBackgroundCarotid artery stenting (CAS) is being increasingly used as an alternative revascularization procedure to carotid endarterectomy; however, subclinical ischemic cerebral lesions after CAS remain as a matter of concern. Hence, we aimed to assess the clinical utility of the CHADS2 score in predicting subclinical ischemic events after CAS.MethodsWe prospectively evaluated 107 patients (mean age: 70.4 ± 6.6 years, male:77) who underwent CAS for carotid artery revascularization. The patients having symptomatic transient ischemic attack or stroke after CAS were excluded. The presence of new hyperintense lesion on diffusion‐weighted imaging (DWI) without any neurological findings was considered as silent ischemia. Patients were classified into two groups as DWI‐positive and DWI‐negative patients.ResultsAmong study population, 28 patients (26.2%) had subclinical embolism. The DWI‐positive group had a significantly higher CHADS2 scores, older age, more frequent history of stroke, higher proportion of type III aortic arch, and longer fluoroscopy time than the DWI‐negative group. Increased CHADS2 score was identified as one of the independent predictors of silent embolism (OR = 5.584; 95%CI: 1.516–20.566; p = .010), and CHADS2 score higher than 2.5 predicted subclinical cerebral ischemia with a sensitivity of 72% and a specificity of 71% (AUC: 0.793; 95% CI: 0.696 – 0.890; p < .001).ConclusionsCHADS2 score was able to predict the risk of periprocedural subclinical ischemic events in CAS and might be of clinical value in the management of patients with carotid artery stenosis.
Improving Electrochemical Cycling Stability of Ni‐rich LiNi0.91Co0.06Al0.03O2 Cathode Materials through H3BO3 and Y2O3 Composite Coating
Authors: Xu Ke , Kou Liang , Zhang Cheng , Zhang Chao , Sun Jing , Tian Zhanyuan , Zhang Jing , You Caiyin 
Published: October 2020

AbstractThe nickel‐rich LiNi0.91Co0.06Al0.03O2 cathode material has attracted wide attention due to its high energy density and appropriate thermal stability; however, its practical application has been greatly restricted by exorbitant residual LiOH/Li2CO3 and poor cycling performance. In this work, we have reduced the residual LiOH/Li2CO3 by washing the LiNi0.91Co0.06Al0.03O2 fabricated through a high‐temperature solid‐state method and improved the cycling performance with a composite coating process. The results show that the amount of residual LiOH/Li2CO3 in the washed materials is significantly decreased, which is very favorable for the processability of materials. LiNi0.91Co0.06Al0.03O2 with 0.1 wt% composite coating exhibits optimum properties: the discharge capacity reaches 217.4 mAh/g at 0.1 C, the cycling retention still remains at 93.7 % after 100 cycles at 1 C, and the cycling retention of the soft‐packing battery remains as high as 81.7 % after 800 cycles at 1 C. The excellent electrochemical performances are attributed to the synergistic effect of excellent fluidity of H3BO3 and outstanding stability of Y2O3, mitigating the interface reaction between electrolyte and cathode material.