bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel human colon signet-ring cell carcinoma organoid line: establishment, characterization and application
Authors: Li Yaqi , Wang Renjie , Huang Dan , Ma Xiaoji , Mo Shaobo , Guo Qiang , Fu Guoxiang , Li Yuanchuang , Xu Xiaoya , Hu Xiang , Zhou Yi , Deng Yun , Zhang Long , Chen Honghong , Gao Jianjun , Zhang Zhen , Cai Sanjun , Hua Guoqiang , Peng Junjie 
Published: October 2019

Abstract
Colon signet-ring cell carcinoma (SRCC) is a rare type of malignant dedifferentiated adenocarcinomas, and is associated with poor survival. However, an in-depth study of the biological features of SRCC is hindered by the lack of a reliable in vitro model of colon SRCC. Thus, the establishment of cell cultures from SRCC has become the most challenging task. Here, by harnessing the power of the organoid culture system, we describe the establishment of a human colon SRCC organoid line from a surgical sample from one patient with colon SRCC. The colon SRCC organoid line, YQ-173, was characterized for morphology, histology, ultrastructure and chromosome stability levels, showing that it resembles the histological and growth characteristics of the original tumor cells; xenografts were used to show that it also has a high tumor formation rate. RNA sequencing of YQ-173 compared with the normal tissue verified its mucinous nature. Capture-based targeted DNA sequencing combined with drug screening based on a bespoke 88 compound library identified that JAK2 might be a treatment target. An in vitro drug screening found that AT9283 and Pacritinib could be effective JAK2 inhibitors, which was consistent with the in vivo xenograft response. We report, for the first time, the establishment of an SRCC organoid line allowing in-depth study of SRCC biology, as well as a strategy to assess in vitro drug testing in a personalized fashion.
Sex-Based Differences in Cortical and Subcortical Development in 436 Individuals Aged 4–54 Years
Authors: Duerden Emma G , Chakravarty M Mallar , Lerch Jason P , Taylor Margot J 
Published: October 2019

Abstract
Sex-based differences in brain development have long been established in ex vivo studies. Recent in vivo studies using magnetic resonance imaging (MRI) have offered considerable insight into sex-based variations in brain maturation. However, reports of sex-based differences in cortical volumes and thickness are inconsistent. We examined brain maturation in a cross-sectional, single-site cohort of 436 individuals (201 [46%] males) aged 4–54 years (median = 16 years). Cortical thickness, cortical surface area, subcortical surface area, volumes of the cerebral cortex, white matter (WM), cortical and subcortical gray matter (GM), including the thalamic subnuclei, basal ganglia, and hippocampi were calculated using automatic segmentation pipelines. Subcortical structures demonstrated distinct curvilinear trajectories from the cortex, in both volumetric maturation and surface-area expansion in relation to age. Surface-area analysis indicated that dorsal regions of the thalamus, globus pallidus and striatum, regions demonstrating structural connectivity with frontoparietal cortices, exhibited extensive expansion with age, and were inversely related to changes seen in cortical maturation, which contracted with age. Furthermore, surface-area expansion was more robust in males in comparison to females. Age- and sex-related maturational changes may reflect alterations in dendritic and synaptic architecture known to occur during development from early childhood through to mid-adulthood.
Limits on Contractual Freedom
Authors: Bridge Michael 
Published: October 2019

Abstract
This article is concerned with an important feature of English law that distinguishes it sharply from civilian systems of law and, to a lesser extent, from other common law systems—namely, its eschewal of an imposed duty of good faith and fair dealing. It will be shown that English law is receptive to such a standard in particular cases but that much of the ground that may be thought to be covered by good faith and fair dealing is covered instead by controls on the exercise of contractual discretion by reference to an implied contractual term that the discretion not be exercised in a way that is capricious, arbitrary, or irrational. This body of law has grown up in the last 30 years or so and has been influenced to a degree by public law considerations.
Differential Impacts of Emerald Ash Borer (Agrilus planipennis Fairmaire) on Forest Communities Containing Native Ash (Fraxinus spp.) Species in Eastern North America
Authors: Granger Joshua J , Zobel John M , Buckley David S 
Published: October 2019

Abstract
Invasion by emerald ash borer (EAB; Agrilus planipennis Fairmaire), an exotic pest, is disrupting natural processes and hindering our ability to conserve genetic diversity and perpetuate ash ecosystem services and functions. The goal of this project was to identify forest communities most at risk in terms of having altered ecosystems following EAB invasion. Using recent data available from the USDA, Forest Service, Forest Inventory and Analysis program, we calculated Shannon–Wiener species diversity index values for forests containing each of the six native ash species found in the eastern United States. These forest communities differed in their species diversity index values, and diversity indices varied across ecosystem provinces and states. In addition, communities containing ashes that are restricted by a narrow range of suitable environmental conditions consistently had lower diversity index values than communities of ash species with greater geographical ranges. Finally, forest stand cohorts (i.e., seedlings, saplings, and canopy trees) had similar species diversity indices across forest communities containing a single ash species. The tree species diversity index values quantified for communities with a single ash species will provide managers with additional information for prioritizing EAB control measures and restoration efforts following EAB invasions.
Quantifying and Classifying Physical Resilience Among Older Adults: The Health, Aging, and Body Composition Study
Authors: Wu Chenkai , Li Ya-Xi , Marron Megan M , Odden Michelle C , Newman Anne B , Sanders Jason L 
Published: October 2019

Abstract

Background
The concept of resilience has gained increasing attention in aging research; however, current literature lacks consensus on how to measure resilience. We constructed a novel resilience measure based on the degree of mismatch between persons’ frailty level and disease burden and examined its predictive validity. We also sought to explore the physiological correlates of resilience.

Methods
Participants were 2,457 older adults from the Health, Aging, and Body Composition Study. We constructed the resilience measure as the residual taken from the linear model regressing frailty on age, sex, race/ethnicity, 14 diseases, self-reported health, and number of medications. Participants were classified into three groups—adapters, expected agers, and premature frailers—based on residuals (less than, within, or above one standard deviation of the mean). Validation outcomes included years of able life (YAL), years of healthy life (YHL), years of healthy and able life (YHAL), disability, hospitalization, and survival.

Results
The average YHAL was 5.1, 7.7, and 9.1 years among premature frailers, expected agers, and adapters, respectively. Compared with premature frailers and expected agers, adapters had significantly lower rates of disability, hospitalization, and mortality and higher proportion surviving to 90 years. The likelihood of surviving to 90 years was 20.4%, 30.6%, and 39.7% among premature frailers, expected agers, and adapters.

Conclusions
We developed and validated a novel approach for quantifying and classifying physical resilience in a cohort of well-functioning white and black older adults. Persons with high physical resilience level had longer healthy life span and lower rates of adverse outcomes.
Comparing EWGSOP2 and FNIH Sarcopenia Definitions: Agreement and Three-Year Survival Prognostic Value in Older Hospitalized Adults. The GLISTEN Study
Authors: Bianchi Lara , Maietti Elisa , Abete Pasquale , Bellelli Giuseppe , Bo Mario , Cherubini Antonio , Corica Francesco , Di Bari Mauro , Maggio Marcello , Martone Anna Maria , Rizzo Maria Rosaria , Rossi Andrea P , Volpato Stefano , Landi Francesco ,   GLISTEN Group Investigators
Published: October 2019

Abstract

Background
Sarcopenia is common among older hospitalized adults but estimates vary according to definitions used. Aims of this study were to investigate the agreement between the European Working Group on Sarcopenia in Older People (EWGSOP2) and the Foundation for the National Institutes of Health (FNIH) Sarcopenia Project criteria and to compare the predictive value of both definitions for 3-year mortality.

Methods
Analysis was performed on 610 older hospitalized patients enrolled in the GLISTEN study. Participants were categorized as sarcopenic or not sarcopenic according to EWGSOP2 and FNIH definitions separately and in a four-group variable (neither criterion positive, only EWGSOP2, only FNIH, both criteria).

Results
Sarcopenia prevalence was 22.8% and 23.9% using EWGSOP2 and FNIH criteria respectively, with a low classification agreement (Cohen’s kappa statistic: 0.29). Sarcopenic participants by each definitions had higher mortality rate when compared to those not sarcopenic (both log-rank test: p<0.001). Participants that met both positive criteria had the shorter survival as compared with the other three groups. Cox models showed that, after adjustment for potential confounders, only EWGSOP2 definition predicted 3-years mortality (HR 1.84; 95%C.I. 1.33-2.57). When the four-group variable was used, compared with the NO EWGSOP2/NO FNIH group, significant mortality risk was found for the EWGSOP2 (HR 2.08; 95%C.I. 1.38-3.16) and the combined EWGSOP2/FNIH group (HR 1.75; 95%C.I. 1.11-2.79).

Conclusions
Agreement between EWGSOP2 and FNIH definitions is poor. Sarcopenia on hospital admission is associated with increased risk of 3-year mortality and EWGSOP2 criteria seem to have the highest predictive value.
Immune Function, Oxidative, and Inflammatory Markers in Centenarians as Potential Predictors of Survival and Indicators of Recovery After Hospital Admission
Authors: Martínez De Toda Irene , Vida Carmen , García-Salmones Marta , Alonso-Fernández Patricia , De La Fuente Mónica 
Published: October 2019

Abstract
Several parameters of immune function, oxidative, and inflammatory stresses have been proposed as markers of health and predictors of longevity and mortality. However, it is unknown if any of these parameters can be used as predictors of survival in centenarians. Therefore, in a group of 27 centenarians, at the time of admission to the Clinical Hospital of Madrid, a series of immune function, antioxidant, oxidant, and inflammatory parameters were studied. Some centenarians survived and others did not, thus establishing two groups, “survivors” (n = 9) and “nonsurvivors” (n = 18). The results show that surviving centenarians display higher neutrophil chemotaxis and microbicidal capacity, natural killer activity, lymphoproliferation, glutathione reductase activity, and basal interleukin-10 release. Moreover, lower neutrophil and lymphocyte adherence, superoxide anion and malondialdehyde concentrations, and basal release of tumor necrosis factor α are also reported. The odds ratios for survival for these parameters were also calculated, with the highest odds ratios being the lymphoproliferative capacity and the ex vivo basal and stimulated release of interleukin-6 from mononuclear cells (odds ratio = 136.00). Therefore, these parameters have the potential to be used in the clinical setting as predictors of survival in centenarians. In the survivors group, the same parameters were also analyzed after 3 months. Because survivors showed an increase in neutrophil and lymphocyte chemotaxis capacity during the recovery period, reaching similar values to those observed in healthy centenarians, these parameters could be proposed as indicators of recovery.
Mortality Risk Among Older People Who Did Versus Did Not Sustain a Fracture: Baseline Prefracture Strength and Gait Speed as Predictors in a 15-Year Follow-Up
Authors: Koivunen Kaisa , Sillanpää Elina , von Bonsdorff Mikaela , Sakari Ritva , Törmäkangas Timo , Rantanen Taina 
Published: October 2019

Abstract

Background
Physiological reserve, as indicated by muscle strength and gait speed, may be especially determinant of survival in people who are exposed to a health stressor. We studied whether the association between strength/speed and mortality risk would be stronger in the time period after a fracture compared to other time periods.

Methods
Participants were population-based sample of 157 men and 325 women aged 75 and 80 years at baseline. Maximal 10-m gait speed and maximal isometric grip and knee extension strength were tested at the baseline before the fracture. Subsequent fracture incidence and mortality were followed up for 15 years. Cox regression analysis was used to estimate fracture time-stratified effects of gait speed and muscle strength on mortality risk in three states: (i) nonfracture state, (ii) the first postfracture year, and (iii) after the first postfracture year until death/end of follow-up.

Results
During the follow-up, 20% of the men and 44% of the women sustained a fracture. In both sexes, lower gait speed and in women lower knee extension strength was associated with increased mortality risk in the nonfracture state. During the first postfracture year, the mortality risk associated with slower gait and lower strength was increased and higher than in the nonfracture state. After the first postfracture year, mortality risk associated with lower gait speed and muscle strength attenuated.

Conclusions
Lower gait speed and muscle strength were more strongly associated with mortality risk after fracture than during nonfracture time, which may indicate decreased likelihood of recovery.
High resolution ambient noise tomography of the Southwestern Alps and the Ligurian margin
Authors: Guerin Gauthier , Rivet Diane , Deschamps Anne , Larroque Christophe , Mordret Aurélien , Dessa Jean-Xavier , Martin Xavier 
Published: October 2019

SUMMARYThe Southwestern Alps and the Ligurian margin is a region of moderate seismicity with a high rate of small to moderate events. Identifying the active faults in this very densely populated region is critical to better assess the hazard and mitigate the risk. An accurate 3-D velocity model of the shallow to middle crust is a fundamental step to better locate the seismicity, and hence, the faults from which it originates.We performed ambient noise surface-wave tomography based on all available continuous seismological data from the French and Italian permanent networks (RESIF, INGV, RSNI), and current and past temporary experiments (AlpArray, CASSAT, SISVAR, RISVAL). In addition to these available data, we deployed three more stations to improve the spatial resolution in a region with sparse seismic station coverage. Overall, we used 55 inland seismic stations, 5 oceans bottom seismometers and 2 offshore cabled site/sensors. Data span the 2014–2018 time period. Time series from all available components were cross-correlated to reconstruct both Rayleigh and Love-wave Green's functions. For each station-pair Rayleigh and Love group velocity dispersion curves were semi-automatically picked using a frequency–time analysis. Then we regionalize these group velocities to build 2-D Rayleigh and Love velocity-maps between 1.5 and 9 s period. Using a two-step inversion, we estimate the best 3-D shear wave velocity model. The first step is based on a Neighbourhood Algorithm to recover the best three layers’ velocity model at each cell of the model. We then use this three-layer model as a starting model in a perturbational method based on finite elements. At periods up to 5 s, the spatial variation of the velocity is well correlated with the effective geology of the area. Lower velocities are observed in areas where the sedimentary cover is thicker, such as the Var and Paillon valley near Nice, or in the subalpine domain in the northwestern part of the region. Higher velocities are retrieved in areas where massifs are present, such as the Argentera-Mercantour massifs in the northeastern, or the Esterel massif in the southwestern part of the region.
Between midnight and republic: Theory and practice of India’s Dominion status
Authors: De Rohit 
Published: October 2019

Abstract
India became independent on August 15, 1947, and became a democratic republic on January 26, 1950. For the three years in between it functioned as a British dominion, where unelected Indian nationalist leaders were administered oaths in the name of the King-Emperor by a British Viceroy. While this was a critical period for establishing the Indian state, as borders were fixed, populations exchanged, industries set up, electoral lists created, and the constitution written, the legal infrastructure of dominionhood has been ignored both in scholarly literature and in political writings. Central to the article is the problem that Dominion status creates for legal temporality, a gray zone between colony and a republic. This article excavates this neglected period, arguing that it is critical to understanding both the endurance of India’s postcolonial constitution and democracy and the legal processes of decolonization within the British Empire. The article examines the peculiarities of the debate over dominion status for India after World War I. Within the British Empire, India had a legal status somewhat less than dominion but higher than a colony, due to the failure to accommodate racial difference within imperial federalism. It then investigates the reasons behind the British government and Indian nationalists both accepting Dominion status in 1947 despite having opposed it for almost two decades. Finally, it examines how the “dominion period” is both a problem and a resource for the judicial construction of time and constitutional legitimacy in republican India.
Impact of Universal Antiretroviral Treatment Eligibility on Rapid Treatment Initiation Among Young Adolescents with Human Immunodeficiency Virus in Sub-Saharan Africa
Authors: Tymejczyk Olga , Brazier Ellen , Wools-Kaloustian Kara , Davies Mary-Ann , Dilorenzo Madeline , Edmonds Andrew , Vreeman Rachel , Bolton Carolyn , Twizere Christella , Okoko Nicollate , Phiri Sam , Nakigozi Gertrude , Lelo Patricia , von Groote Per , Sohn Annette H , Nash Denis 
Published: October 2019

Abstract

Background
Young adolescents with perinatally acquired human immunodeficiency virus (HIV) are at risk for poor care outcomes. We examined whether universal antiretroviral treatment (ART) eligibility policies (Treat All) improved rapid ART initiation after care enrollment among 10–14-year-olds in 7 sub-Saharan African countries.

Methods
Regression discontinuity analysis and data for 6912 patients aged 10–14-years were used to estimate changes in rapid ART initiation (within 30 days of care enrollment) after adoption of Treat All policies in 2 groups of countries: Uganda and Zambia (policy adopted in 2013) and Burundi, Democratic Republic of the Congo, Kenya, Malawi, and Rwanda (policy adopted in 2016).

Results
There were immediate increases in rapid ART initiation among young adolescents after national adoption of Treat All. Increases were greater in countries adopting the policy in 2016 than in those adopting it in 2013: 23.4 percentage points (pp) (95% confidence interval, 13.9–32.8) versus 11.2pp (2.5–19.9). However, the rate of increase in rapid ART initiation among 10–14-year-olds rose appreciably in countries with earlier treatment expansions, from 1.5pp per year before Treat All to 7.7pp per year afterward.

Conclusions
Universal ART eligibility has increased rapid treatment initiation among young adolescents enrolling in HIV care. Further research should assess their retention in care and viral suppression under Treat All.
Trends in type 1 diabetes diagnosis in Ghana
Authors: Sarfo-Kantanka Osei , Asamoah-Boaheng Michael , Arthur Joshua , Agyei Martin , Barnes Nana Ama , Tenkorang Eric Y , Midodzi William 
Published: October 2019

Abstract

Background
Despite the fact that the rate of type 1 diabetes (T1D) is increasing worldwide, there exists a dearth of information on the disease in most sub-Saharan African countries. The goal of this study was to determine the enrolment trend of T1D using data compiled over 28 y from a teaching hospital in Kumasi, Ghana.

Methods
Information collected included sex, age at diagnosis and date of T1D diagnosis. We identified trends from 1992 to 2018, divided into 3 y intervals.

Results
From 1992 to 2018, 1717 individuals with T1D were enrolled in the diabetes clinic at the Komfo Anokye Teaching Hospital. The male:female ratio was 1:1.2. The number of individuals diagnosed with T1D decreased among the 10–19 y age group during the 1992–1994 period, followed by a progressive increase within the same age group during the subsequent period (from 35.4% in 1995–1997 to 63.2% in 2016–2018). There was a decline in the proportion of children 0–9 y of age diagnosed during the study period (from 5.1% in 1992–1994 to 3.6% in 2016–2018).

Conclusions
In our study population, a decreasing trend of T1D enrolments was observed in general while among adolescents an increasing trend was observed.
An integrated web application for decision support and automation of EHR workflow: a case study of current challenges to standards-based messaging and scalability from the EMBED trial
Authors: Melnick Edward R , Holland Wesley C , Ahmed Osama M , Ma Anthony K , Michael Sean S , Goldberg Howard S , Lagier Christian , D’Onofrio Gail , Stachowiak Tomek , Brandt Cynthia , Solad Yauheni 
Published: October 2019

Abstract
Computerized clinical decision support (CDS) faces challenges to interoperability and scalability. Centralized, web-based solutions offer a mechanism to share the cost of CDS development, maintenance, and implementation across practices. Data standards have emerged to facilitate interoperability and rapid integration of such third-party CDS. This case report describes the challenges to implementation and scalability of an integrated, web-based CDS intervention for EMergency department-initiated BuprenorphinE for opioid use Disorder which will soon be evaluated in a trial across 20 sites in five healthcare systems. Due to limitations of current standards, security concerns, and the need for resource-intensive local customization, barriers persist related to centralized CDS at this scale. These challenges demonstrate the need and importance for future standards to support two-way messaging (read and write) between electronic health records and web applications, thus allowing for more robust sharing across health systems and decreasing redundant, resource-intensive CDS development at individual sites.