bluuebunny/crossref_metadata_2025_split huggingface dataset:
Illness perceptions of Turkish Cypriot patients receiving haemodialysis: A qualitative study
Authors: Keskindag Buse , Farrington Ken , Oygar Duriye D. , Mertan Biran , Hucker Abigail , Sharma Shivani 
Published: October 2020

AbstractBackgroundIllness perceptions refer to cognitive appraisals that help patients understand and make sense of their condition. Although their importance in health behaviour and outcomes has been evidenced, less is known about cultural influences on mental representations of kidney failure amongst patients receiving haemodialysis in different settings.ObjectiveTo explore the illness perceptions of Turkish patients receiving haemodialysis in North Cyprus (Turkish Cypriots).DesignA qualitative study involving individual semistructured interviews.ParticipantsFourteen patients receiving haemodialysis, recruited from three state hospitals in North Cyprus.ApproachAll interviews were conducted in Turkish, audio‐recorded, and transcribed verbatim. They were analysed inductively in the original language using reflexive thematic analysis. Once the analysis was completed, it was translated into English. Quality assurance was integral to the research process to retain semantic equivalence.FindingsThree themes were developed. “Illness appraisal” highlighted a lack of factual knowledge about kidney failure and how this is related to attempts at sense‐making, whilst retaining hope for the future. “Life‐changing effects” centred around the negative consequences of haemodialysis across multiple domains (e.g., emotional and physical). “Active coping strategies” focused on mechanisms that patients adopt to manage the burden of haemodialysis, particularly approaches that are culturally rooted.ConclusionThere is a need for better communication to address the lack of individual patient knowledge about kidney failure. Haemodialysis is described as a burdensome treatment though existing coping mechanisms suggest that psycho‐spiritual interventions may be advantageous to aid adjustment for Turkish Cypriots receiving haemodialysis.
Sociolinguistic labor, linguistic climate, and race(ism) on campus: Black college students’ experiences with language at predominantly white institutions
Authors: Holliday Nicole R. , Squires Lauren 
Published: October 2020

AbstractThis project explores the sociolinguistic experiences of black American students in predominantly/historically white higher education settings. Through interviews with 30 black undergraduates at two different types of institutions, we show how language is a salient factor in racialization and racism on American college campuses. Both sets of students discussed stereotype threat (being at risk of negative stereotyping based on their language), as well as bifurcated sociolinguistic identities (an outcome of managing their linguistic resources to avoid negative stereotyping). We also find that the nuances of students’ racialized experiences with language differ depending on other elements of campus climate: at the small private college, more students described tensions between black students, and stringent expectations for hyper‐‘academic’ language. Student accounts reveal thesociolinguistic laborthey perform in navigating campus environments rife with linguistic racism, showing that campus climate includeslinguistic climate, undergirded by raciolinguistic ideologies.
Health Care Disparities Across the Urban‐Rural Divide: A National Study of Individuals with COPD
Authors: Gaffney Adam W. , Hawks Laura , White Alexander C. , Woolhandler Steffie , Himmelstein David , Christiani David C. , McCormick Danny 
Published: October 2020

AbstractPurposeThe burden of chronic obstructive pulmonary disease (COPD) is high in rural America. Few studies, however, have examined urban/rural differences in health care access, or racial/ethnic and income disparities stratified by urban/rural residence, among persons with COPD.MethodsWe studied individuals age ≥ 40 years with COPD from the 2018 Behavioral Risk Factor Surveillance System. The primary exposure was “urban” or “rural” county of residence. We examined multiple health and health care access/services outcomes using logistic regressions adjusted for age and sex, and performed analyses stratified by rural/urban county that included additional adjustment for race/ethnicity or income.FindingsOur sample included 34,439 individuals. COPD prevalence was 8.6% in rural counties versus 5.4% in urban counties. Rural residents with COPD were poorer, had less education, worse health, and more disability. Of the rural population with COPD, 12.6% were uninsured, versus 10.4% in urban areas (AOR 1.26; 95% CI: 1.00–1.58). Rural residents with COPD were more likely to have not seen a doctor due to cost (AOR 1.18; 95% CI: 1.02–1.36). Differences in other outcomes were mostly nonsignificant. We observed large access disparities by race/ethnicity and income among individuals in both urban and rural counties, with the highest rates of forgone care among minorities in rural counties.ConclusionPatients with COPD in rural areas experience greater morbidity and obstacles to care than those in urban areas. Racial/ethnic minorities and those with low incomes—particularly in rural areas—are also at greater risk of forgoing doctor visits due to cost. Expanded access to health care could address respiratory health inequities.
Linseed protein concentrate as alternative to fishmeal in diets for silver catfish, Rhamdia quelen
Authors: Pianesso Dirleise , Goulart Fernanda Rodrigues , Adorian Taida Juliana , Mombach Patrícia Inês , de Lima Joziane Soares , dos Santos Thaís Soares , da Silva Leila Picolli 
Published: October 2020

AbstractA 60‐day feeding trial was conducted to evaluate the efficiency of the substitution of fishmeal (FM) with linseed protein concentrate (LPC) in the diet of silver catfish, Rhamdia quelen (6.13 g). Five isonitrogenous and isoenergetic experimental diets were formulated to replace 0, 10, 20, 30, or 40% of FM with LPC, and silver catfish were fed these diets to apparent satiation. LPC had a lower protein content compared to FM, but in vitro results showed higher protein digestibility of LPC as compared to fishmeal. There were no significant differences (p > .05) in growth, digestive somatic index, and intestinal quotient in the groups receiving LPC diets. The activity of the digestive enzymes did not differ among treatments, except for chymotrypsin, which was higher (p < .05) in the 30 and 40% LPC treatment groups. These results show that LPC protein can replace up to 40% of FM in silver catfish diets without adverse effects on growth performance.
Pan‐regional marine benthic cryptobiome biodiversity patterns revealed by metabarcoding Autonomous Reef Monitoring Structures
Authors: Pearman John K. , Chust Guillem , Aylagas Eva , Villarino Ernesto , Watson James R. , Chenuil Anne , Borja Angel , Cahill Abigail E. , Carugati Laura , Danovaro Roberto , David Romain , Irigoien Xabier , Mendibil Iñaki , Moncheva Snejana , Rodríguez‐Ezpeleta Naiara , Uyarra Maria C. , Carvalho Susana 
Published: October 2020

AbstractAutonomous Reef Monitoring Structures (ARMS) have been applied worldwide to characterize the critical yet frequently overlooked biodiversity patterns of marine benthic organisms. In order to disentangle the relevance of environmental factors in benthic patterns, here, through standardized metabarcoding protocols, we analyse sessile and mobile (<2 mm) organisms collected using ARMS deployed across six regions with different environmental conditions (3 sites × 3 replicates per region): Baltic, Western Mediterranean, Adriatic, Black and Red Seas, and the Bay of Biscay. A total of 27,473 Amplicon Sequence Variants (ASVs) were observed ranging from 1,404 in the Black Sea to 9,958 in the Red Sea. No ASVs were shared among all regions. The highest number of shared ASVs was between the Western Mediterranean and the Adriatic Sea (116) and Bay of Biscay (115). Relatively high numbers of ASVs (103), mostly associated with the genus Amphibalanus, were also shared between the lower salinity seas (Baltic and Black Seas). We found that compositional differences in spatial patterns of rocky‐shore benthos are determined slightly more by dispersal limitation than environmental filtering. Dispersal limitation was similar between sessile and mobile groups, while the sessile group had a larger environmental niche breadth than the mobile group. Further, our study can provide a foundation for future evaluations of biodiversity patterns in the cryptobiome, which can contribute up to 70% of the local biodiversity.
Nationalism and populism on the left: The case of Podemos
Authors: Custodi Jacopo 
Published: October 2020

AbstractThis article provides an empirical exploration of the relation between nationalism and populism on the left of the political spectrum. The Spanish party Podemos is a key case study for such an analysis, as it is a left‐populist actor that has made extensive use of nationalist rhetoric in its discourse. Through a discourse analysis on a corpus that includes speeches by Podemos leadership and primary data such as interviews and original unpublished material, this article studies the nationalist dimension of Podemos and its relation with the party's much‐discussed populism. The analysis shows that the Podemos leadership deliberately embeds nationalism in its populist strategy: Nationalism is a central element of the party's populist project and serves to advance an alternative form of national identification that challenges that of the right wing. Through a resignification of national pride and belonging, Podemos constructs an image of Spain that refers to an inclusive welfare state, to people's mobilization, and to a moral community that is not delimited by lingual or ethnic particularisms.
Creation in Aquinas: ex nihilo or ex deo?
Authors: Soars Daniel 
Published: October 2020

AbstractWhile the Christian emphasis on creation as a free and gracious gift is often juxtaposed with Neoplatonic notions of world-production as the emanation of being from the First Cause, I argue in this essay that there is no obvious contradiction between the doctrines of creation ex nihilo and emanation ex deo in Aquinas's thought. This is partly because the Christian teaching that the world is created ‘from nothing’ was never intended to deny that it was from God, but to deny that it was made from anything other than God. By drawing on the Liber de Causis to support his explanation of creation as the emanation of all being from the universal cause, Aquinas provides us with a way to foreground a doctrine which belongs to the foundations of Christian faith but which rarely receives sufficient attention in systematic theology – namely, the omnipresence of the God who is in everything.
The Aeromonas salmonicida plasmidome: a model of modular evolution and genetic diversity
Authors: Vincent Antony T. , Hosseini Nava , Charette Steve J. 
Published: October 2020

AbstractHigh‐throughput genomic sequencing has helped to reveal the plasmidome of Aeromonas salmonicida. This literature review provides an overview of A. salmonicida’s rich plasmidome by presenting all the plasmids identified so far, addressing their biological importance and the functional links between them. The plasmids of A. salmonicida, especially those bearing antibiotic resistance genes, can provide clues about interactions of this species with other pathogens (animals and humans), as is the case for pRAS3‐3432 and Chlamydia suis or pSN254b and Salmonella enterica. In addition to antibiotic resistance, plasmids play an important role in the virulence of A. salmonicida, particularly for the subspecies salmonicida and the plasmid pAsa5, which carries genes for the type‐three secretion system, a virulence factor essential for the bacterium. The A. salmonicida plasmidome also has many cryptic plasmids with no known biological function, but which can be used for the acquisition of new genetic elements. Striking examples are pAsa7 and pAsaXII that provide, respectively, resistance to chloramphenicol and formaldehyde and are derivatives of cryptic pAsa2.
Modeling the neuromuscular junction in vitro: an approach to study neuromuscular junction disorders
Authors: Castellanos‐Montiel María José , Velasco Iván , Escobedo‐Avila Itzel 
Published: October 2020

AbstractThe neuromuscular junction (NMJ) is a specialized structure that works as an interface to translate the action potential of the presynaptic motor neuron (MN) in the contraction of the postsynaptic myofiber. The design of appropriate experimental models is essential to have efficient and reliable approaches to study NMJ development and function, but also to generate conditions that recapitulate distinct features of diseases. Initial studies relied on the use of tissue slices maintained under the same environment and in which single motor axons were difficult to trace. Later, MNs and muscle cells were obtained from primary cultures or differentiation of progenitors and cocultured as monolayers; however, the tissue architecture was lost. Current approaches include self‐assembling 3D structures or the incorporation of biomaterials with cells to generate engineered tissues, although the incorporation of Schwann cells remains a challenge. Thus, numerous investigations have established different NMJ models, some of which are quite complex and challenging. Our review summarizes the in vitro models that have emerged in recent years to coculture MNs and skeletal muscle, trying to mimic the healthy and diseased NMJ. We expect our review may serve as a reference for choosing the appropriate experimental model for the required purposes of investigation.
Multiple factors are involved in regulation of extracellular membrane vesicle biogenesis in Streptococcus mutans
Authors: Wen Zezhang T. , Jorgensen Ashton N. , Huang Xiaochang , Ellepola Kassapa , Chapman Lynne , Wu Hui , Jeannine Brady L. 
Published: October 2020

AbstractStreptococcus mutans, a major etiological agent of human dental caries, produces membrane vesicles (MVs) that contain protein and extracellular DNA. In this study, functional genomics, along with in vitro biofilm models, was used to identify factors that regulate MV biogenesis. Our results showed that when added to growth medium, MVs significantly enhanced biofilm formation by S. mutans, especially during growth in sucrose. This effect occurred in the presence and absence of added human saliva. Functional genomics revealed several genes, including sfp, which have a major effect on S. mutans MVs. In Bacillus sp. sfp encodes a 4′‐phosphopantetheinyl transferase that contributes to surfactin biosynthesis and impacts vesiculogenesis. In S. mutans, sfp resides within the TnSmu2 Genomic Island that supports pigment production associated with oxidative stress tolerance. Compared to the UA159 parent, the Δsfp mutant, TW406, demonstrated a 1.74‐fold (p < .05) higher MV yield as measured by BCA protein assay. This mutant also displayed increased susceptibility to low pH and oxidative stressors, as demonstrated by acid killing and hydrogen peroxide challenge assays. Deficiency of bacA, a putative surfactin synthetase homolog within TnSmu2, and especially dac and pdeA that encode a di‐adenylyl cyclase and a phosphodiesterase, respectively, also significantly increased MV yield (p < .05). However, elimination of bacA2, a bacitracin synthetase homolog, resulted in a >1.5‐fold (p < .05) reduction of MV yield. These results demonstrate that S. mutans MV properties are regulated by genes within and outside of the TnSmu2 island, and that as a major particulate component of the biofilm matrix, MVs significantly influence biofilm formation.
Subjective oral symptoms are insufficient predictors of a positive oral food challenge
Authors: Nachshon Liat , Zipper Oren , Levy Michael B. , Goldberg Michael R. , Epstein‐Rigby Naama , Elizur Arnon 
Published: October 2020

AbstractBackgroundSubjective oral symptoms, especially if recurrent, might lead to termination of an oral food challenge (OFC) for fear of a subsequent severe reaction.MethodsIn a single‐center retrospective cohort study, oral food challenges to milk, egg, peanut, sesame, or tree nuts performed between January 2016 and January 2018 in patients aged ≥3 years, at the Institute of Allergy, Immunology and Pediatric Pulmonology in Shamir Medical Center, were analyzed. Subjective oral symptoms during the challenge were documented, and their association with the challenge outcome was examined.ResultsA total of 323 patients underwent 652 oral food challenges to the investigated foods (milk, 71; egg, 22; peanut, 48; sesame, 24; and tree nuts, 487). Subjective oral symptoms were experienced in 237 (36.3%) of all OFCs performed, and their rate was comparable across most foods tested. The rate of positive challenges was significantly higher when subjective oral symptoms were experienced during the challenge than when they were not (69.6% vs 30.4%, respectively, P < .001). However, the false‐positive rate of such symptoms in predicting a positive food challenge was 30.3% for all foods; peanut, 40%; sesame, 27.3%; milk, 35.5%; egg, 33%; and tree nuts, 28.4%. Overall, subjective oral symptoms (SOS) provided sensitivity of 56.7, specificity 80.4, PPV 69.6, and NPV 69.0 for predicting OFC outcome. Importantly, reactions during positive challenges with and without subjective oral symptoms were comparable to severity.ConclusionsContinuing OFCs with subjective oral symptoms is recommended for it will prevent false‐positive results in a third of cases without increasing patients' risk.
Duration of neonatal intensive care unit exposure associated with decreased risk of atopic dermatitis
Authors: Schoch Jennifer J. , Miranda Nicole , Garvan Cynthia W. , Monir Reesa L. , Neu Josef , Lemas Dominick J. 
Published: October 2020

AbstractBackground/ObjectivesPremature infants have lower rates of atopic dermatitis (AD) compared with full‐term infants, though little is known about the factors contributing to this association. We explored the infant and environmental factors that may contribute to the association between prematurity and atopic dermatitis, including mode of delivery, birthweight, gestation, and duration of stay in the neonatal intensive care unit (NICU).MethodsThis was a single‐center retrospective study. Independent samples t tests or chi‐square tests were used to compare groups on continuous and categorical variables, respectively. Logistic regression then examined the association of the predictor variables with AD.ResultsFour thousand sixteen mother‐infant dyads were included. Infants had a higher risk of developing AD if they were delivered vaginally (P = .013), did not stay in the NICU (P < .001), had a longer gestation (P = .001), or had a higher birthweight (P = .002). In modeling atopic dermatitis with the predictor variables, only NICU length of stay remained significantly associated with a lower risk of AD (P = .004).ConclusionInfants had a lower risk of developing AD if they had a longer stay in the NICU.
Mutational analysis of Arabidopsis thaliana ABCE2 identifies important motifs for its RNA silencing suppressor function
Authors: Mõttus J. , Maiste S. , Eek P. , Truve E. , Sarmiento C. 
Published: October 2020

Abstract

ATP‐binding cassette sub‐family E member 1 (ABCE1) is recognized as a strongly conserved ribosome recycling factor, indispensable for translation in archaea and eukaryotes, however, its role in plants remains largely unidentified. Arabidopsis thaliana encodes two paralogous ABCE proteins (AtABCE1 and AtABCE2), sharing 81% identity. We previously reported that AtABCE2 functions as a suppressor of RNA silencing and that its gene is ubiquitously expressed. Here we describe the structural requirements of AtABCE2 for its suppressor function.

Using agroinfiltration assays, we transiently overexpressed mutated versions of AtABCE2 together with GFP, to induce silencing in GFP transgenic Nicotiana benthamiana leaves. The influence of mutations was analysed at both local and systemic levels by in vivo imaging of GFP, Northern blot analysis of GFP siRNAs and observation of plants under UV light.

Mutants of AtABCE2 with impaired ATP binding in either active site I or II failed to suppress GFP RNA silencing. Mutations disrupting ATP hydrolysis influenced the suppression of silencing differently at active site I or II. We also found that the N‐terminal iron–sulphur cluster domain of AtABCE2 is crucial for its suppressor function.

Meaningfully, the observed structural requirements of AtABCE2 for RNA silencing suppression were found to be similar to those of archaeal ABCE1 needed for ribosome recycling. AtABCE2 might therefore suppress RNA silencing via supporting the competing RNA degradation mechanisms associated with ribosome recycling.
African Political Elites and the Making(s) of the China Model in Africa
Authors: Hodzi Obert 
Published: October 2020

States in Africa are labeled “system ineffectual,” inconsequential to global politics, and lacking material and ideational capabilities to structure their foreign relations. The result is a portrayal of a passive Africa at the whims of its bilateral partners—be they China, the United States, or Russia. Contra this impolitic framing of Africa, this article explores how states in Africa are strategically shaping the norms of development paradigms promoted by foreign powers and influencing external actors to legitimize their preferred norms. Empirically focusing on China's development model in Africa, the article examines how norms related to the model are being shaped and diffused; and how through the ingenuity of political elites, China is making the China model “on the go.” The main argument is that political elites in Africa are strategically reconstructing the China model, creating their own multifaceted versions that delicately balance their own domestic interests and their bilateral relations with China while deriving maximum benefits from the People's Republic of China.Related Articles (in this Special Issue)Duggan, Niall. 2020. “China—The Champion of the Developing World: A Study of China's new Development Model and its Role in Changing Global Economic Governance.” Politics & Policy 48 (5).Ganda, Willie D. 2020. “The China Model in Zimbabwe: The Belt and Road Initiative and Beyond.” Politics & Policy 48 (5).Hodzi, Obert, and John H. S. Åberg. 2020. “Introduction to the Special Issue: Strategic Deployment of the China Model in Africa.” Politics & Policy 48 (5).
Multivariate analysis reveals a generalizable human electrophysiological signature of working memory load
Authors: Adam Kirsten C. S. , Vogel Edward K. , Awh Edward 
Published: October 2020

AbstractWorking memory (WM) is an online memory system that is critical for holding information in a rapidly accessible state during ongoing cognitive processing. Thus, there is strong value in methods that provide a temporally resolved index of WM load. While univariate EEG signals have been identified that vary with WM load, recent advances in multivariate analytic approaches suggest that there may be rich sources of information that do not generate reliable univariate signatures. Here, using data from four published studies (n = 286 and >250,000 trials), we demonstrate that multivariate analysis of EEG voltage topography provides a sensitive index of the number of items stored in WM that generalizes to novel human observers. Moreover, multivariate load detection (“mvLoad”) can provide robust information at the single‐trial level, exceeding the sensitivity of extant univariate approaches. We show that this method tracks WM load in a manner that is (1) independent of the spatial position of the memoranda, (2) precise enough to differentiate item‐by‐item increments in the number of stored items, (3) generalizable across distinct tasks and stimulus displays, and (4) correlated with individual differences in WM behavior. Thus, this approach provides a powerful complement to univariate analytic approaches, enabling temporally resolved tracking of online memory storage in humans.