bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regional differences between superficial and deep lumbar multifidus in patients with chronic lumbar spine pathology
Authors: Padwal Jennifer , Berry David B. , Hubbard James C. , Zlomislic Vinko , Allen R. Todd , Garfin Steven R. , Ward Samuel R. , Shahidi Bahar 
Published: November 2020

AbstractBackgroundDue to its unique arrangement, the deep and superficial fibers of the multifidus may have differential roles for maintaining spine stabilization and lumbar posture; the superficial multifidus is responsible for lumbar extension and the deep multifidus for intersegmental stability. In patients with chronic lumbar spine pathology, muscle activation patterns have been shown to be attenuated or delayed in the deep, but not superficial, multifidus. This has been interpreted as pain differentially influencing the deep region. However, it is unclear if degenerative changes affecting the composition and function of the multifidus differs between the superficial and deep regions, an alternative explanation for these electrophysiological changes. Therefore, the goal of this study was to investigate macrostructural and microstructural differences between the superficial and deep regions of the multifidus muscle in patients with lumbar spine pathology.MethodsIn 16 patients undergoing lumbar spinal surgery for degenerative conditions, multifidus biopsies were acquired at two distinct locations: 1) the most superficial portion of muscle adjacent to the spinous process and 2) approximately 1 cm lateral to the spinous process and deeper at the spinolaminar border of the affected vertebral level. Structural features related to muscle function were histologically compared between these superficial and deep regions, including tissue composition, fat fraction, fiber cross sectional area, fiber type, regeneration, degeneration, vascularity and inflammation.ResultsNo significant differences in fat signal fraction, muscle area, fiber cross sectional area, muscle regeneration, muscle degeneration, or vascularization were found between the superficial and deep regions of the multifidus. Total collagen content between the two regions was the same. However, the superficial region of the multifidus was found to have less loose and more dense collagen than the deep region.ConclusionsThe results of our study did not support that the deep region of the multifidus is more degenerated in patients with lumbar spine pathology, as gross degenerative changes in muscle microstructure and macrostructure were the same in the superficial and deep regions of the multifidus. In these patients, the multifidus is not protected in order to maintain mobility and structural stability of the spine.
Dynamic and reversible DNA methylation changes induced by genome separation and merger of polyploid wheat
Authors: Yuan Jingya , Jiao Wu , Liu Yanfeng , Ye Wenxue , Wang Xiue , Liu Bao , Song Qingxin , Chen Z. Jeffrey 
Published: November 2020

AbstractBackgroundWheat is a powerful genetic model for studying polyploid evolution and crop domestication. Hexaploid bread wheat was formed by two rounds of interspecific hybridization and polyploidization, processes which are often accompanied by genetic and epigenetic changes, including DNA methylation. However, the extent and effect of such changes during wheat evolution, particularly from tetraploid-to-hexaploid wheat, are currently elusive.ResultsHere we report genome-wide DNA methylation landscapes in extracted tetraploid wheat (ETW, AABB), natural hexaploid wheat (NHW, AABBDD), resynthesized hexaploid wheat (RHW, AABBDD), natural tetraploid wheat (NTW, AABB), and diploid (DD). In the endosperm, levels of DNA methylation, especially in CHG (H=A, T, or C) context, were dramatically decreased in the ETW relative to natural hexaploid wheat; hypo-differentially methylated regions (DMRs) (850,832) were 24-fold more than hyper-DMRs (35,111). Interestingly, those demethylated regions in ETW were remethylated in the resynthesized hexaploid wheat after the addition of the D genome. In ETW, hypo-DMRs correlated with gene expression, and TEs were demethylated and activated, which could be silenced in the hexaploid wheat. In NHW, groups of TEs were dispersed in genic regions of three subgenomes, which may regulate the expression of TE-associated genes. Further, hypo-DMRs in ETW were associated with reduced H3K9me2 levels and increased expression of histone variant genes, suggesting concerted epigenetic changes after separation from the hexaploid.ConclusionGenome merger and separation provoke dynamic and reversible changes in chromatin and DNA methylation. These changes correlate with altered gene expression and TE activity, which may provide insights into polyploid genome and wheat evolution.
Human trophoblast-derived exosomes attenuate doxorubicin-induced cardiac injury by regulating miR-200b and downstream Zeb1
Authors: Ni Jie , Liu Yihai , Kang Lina , Wang Lian , Han Zhonglin , Wang Kun , Xu Biao , Gu Rong 
Published: November 2020

AbstractHuman trophoblast stem cells (TSCs) have been confirmed to play a cardioprotective role in heart failure. However, whether trophoblast stem cell-derived exosomes (TSC-Exos) can protect cardiomyocytes from doxorubicin (Dox)-induced injury remains unclear. In the present study, TSC-Exos were isolated from the supernatants of human trophoblasts using the ultracentrifugation method and characterized by transmission electron microscopy and western blotting. In vitro, primary cardiomyocytes were subjected to Dox and treated with TSC-Exos, miR-200b mimic or miR-200b inhibitor. Cellular apoptosis was observed by flow cytometry and immunoblotting. In vivo, mice were intraperitoneally injected into Dox to establish a heart failure model. Then, different groups of mice were administered either PBS, adeno-associated virus (AAV)-vector, AAV-miR-200b-inhibitor or TSC-Exos via tail vein injection. Then, the cardiac function, cardiac fibrosis and cardiomyocyte apoptosis in each group were evaluated, and the downstream molecular mechanism was explored. TSC-Exos and miR-200b inhibitor both decreased primary cardiomyocyte apoptosis. Similarly, mice receiving TSC-Exos and AAV-miR-200b inhibitor exhibited improved cardiac function, accompanied by reduced apoptosis and inflammation. The bioinformatic prediction and luciferase reporter results confirmed that Zeb1 was a downstream target of miR-200b and had an antiapoptotic effect. TSC-Exos attenuated doxorubicin-induced cardiac injury by playing antiapoptotic and anti-inflammatory roles. The underlying mechanism could be an increase in Zeb1 expression by the inhibition of miR-200b expression. In summary, this study sheds new light on the application of TSC-Exos as a potential therapeutic tool for heart failure.
Burden of disease and adaptation to life in patients with Crohn’s perianal fistula: a qualitative exploration
Authors: Adegbola Samuel O. , Dibley Lesley , Sahnan Kapil , Wade Tiffany , Verjee Azmina , Sawyer Rachel , Mannick Sameer , McCluskey Damian , Yassin Nuha , Phillips Robin K. S. , Tozer Philip J. , Norton Christine , Hart Ailsa L. 
Published: November 2020

Abstract
Background
Perianal fistulas are a challenging manifestation of Crohn’s disease. Best medical and surgical therapy results in only about a third of patients remaining in remission at one year on maintenance treatment and sustained healing is often elusive. There is little published data on patient perspective of living with the condition or coping strategies in the face of non-curative/non-definitive treatment. We aimed to understand the experience of living with perianal fistula(s) and their impact on quality of life and routine functioning.

Methods
This exploratory qualitative study used purposive sampling to recruit participants with current / previous diagnosis of Crohn’s anal fistulas, from national IBD / bowel disease charities. The “standards for reporting qualitative research” (SRQR) recommendations were followed. Unstructured individual face-to-face interviews were audio recorded, transcribed and analysed thematically. Early themes were reviewed by the study team including patient advocates, clinicians and qualitative researchers.

Results
Twelve interviews were conducted, achieving apparent data saturation. Three broad themes were uncovered: Burden of symptoms; Burden of treatment; and Impact on emotional, physical and social well-being. Each included several sub-themes, with considerable interplay between these. The impact of perianal fistula(s) on patients with CD is intense and wide reaching, negatively affecting intimate, close and social relationships. Fistulas cause losses in life and work-related opportunities, and treatments can be difficult to tolerate.

Conclusion
Crohn’s perianal fistulas exert a heavy negative physical and emotional impact on patients. These findings will inform development of a patient reported outcome measure to assess treatment effectiveness and quality of life for patients living with this challenging condition.
Whether Letrozole could reduce the incidence of early ovary hyperstimulation syndrome after assisted reproductive technology? A systematic review and meta-analysis
Authors: Zhao Jing , Xu Bin , Huang Xi , Yan Yi , Li Yanping 
Published: November 2020

Abstract
Background
Letrozole can significantly decrease the estrogen level, and has been administrated to prevent the incidence of early ovary hyperstimulation syndrome (OHSS). However, the effect of Letrozole on prevention of OHSS reached to controversial conclusions. The present meta-analysis aim to examine whether Letrozole could reduce the incidence of early OHSS after assisted reproductive technology (ART).

Methods
An exhaustive electronic literature search was conducted on MEDLINE, Google Scholar, CNKI and WANFANG MED ONLINE, from inception until May 2018. We include clinical trials that examined the effect of Letrozole on the prevention of early OHSS. The main outcome measures were the incidence of total early OHSS, mild early OHSS, moderate early OHSS, and severe early OHSS.

Results
Eight studies included in the review. Of these, five publications evaluated the effect of Letrozolel on the prevention of total, mild, moderate, and severe OHSS, respectively. The results indicated that there was a significantly decreased incidence of total OHSS with Letrozole compared with control group, and there were no significantly differences in the incidence of mild, moderate, and severe OHSS between study group with Letrozole and control group. Eight studies reported the incidence of moderate + severe OHSS. We found a significant decrease in incidence of moderate + severe OHSS in high-risk women with Letrozole.

Conclusions
Letrozole has no beneficial effect on the prevention of mild, moderate, and severe OHSS, individually; Letrozole should not be considered as the first-line treatment for prevention of OHSS. Further cohort studies are required to explore the effect of Letrozole on the prevention of OHSS.

Plain English Summary
This study aimed to examine whether Letrozole could reduce the incidence of early OHSS after assisted reproductive technology (ART). A meta-analysis including 8 studies was conducted. There were no significantly differences in the incidence of mild, moderate, and severe OHSS between study group with Letrozole and control group. Letrozole has no beneficial effect on the prevention of mild, moderate, and severe OHSS, individually.
Sinonasal analogue HPV related breast multiphenotypic carcinoma, a report of a case with the first description in the breast
Authors: Oramas Diana M. , Bell Diana , Middleton Lavinia P. 
Published: November 2020

Abstract
Background
High grade basal-like breast carcinomas are triple negative, express basal cytokeratins, and are known for the overall poor prognosis and aggressive behavior. HPV related multiphenotypic sino-nasal carcinoma has overlapping histology with basal-like breast carcinomas, but carry the defining feature of association with high risk HPV.

Case presentation
We present a case of a perimenopausal woman with a non-healing ulcerated lesion involving the nipple and breast following a trauma. Biopsy performed showed an HPV-positive basal-like carcinoma with squamous differentiation involving the breast, analogous to multiphenotypic carcinoma previously described in the sinonasal tract.

Conclusion
This is the first report of a case of a high- risk HPV related basal-like carcinoma with squamous differentiation, described in the literature. We highlight the morphology and immunophenotype of this lesion and its recognition when compared to other multiphenotypic lesions of the breast, and suggest that pathologists should consider HPV evaluation when encountering similar basal-like tumors involving the breast.
Analysis of the early clinical outcomes of arthroscopic debridement in the treatment of shoulder tuberculosis
Authors: He Yanwei , Liu Juncai , Wang Zhi , Zhou Peng , Deng Xiangtian , Yang Li , Chen Zan , Li Zhong 
Published: November 2020

AbstractBackgroundDue to atypical clinical symptoms, it is difficult to diagnose joint tuberculosis infection, which often results in misdiagnosis and missed diagnosis. It is easy to cause joint disability. And there are few reports of using arthroscopy to diagnose and treat shoulder tuberculosis. This case series aims to introduce the clinical outcomes of arthroscopic treatment of shoulder tuberculosis.MethodsTwenty-nine patients with shoulder tuberculosis from September 2013 to February 2019 were included (10 males, 19 females; age range from 22 to 69; the average age is 37.6 years). All patients underwent arthroscopic lesion debridement, with preoperative and postoperative regular use of isoniazid, rifampicin, pyrazinamide, and streptomycin quadruple anti-tuberculosis drugs. The erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were recorded before and at the last follow-up. The shoulder function was evaluated according to the visual analogue scoring method (visual analogue scale, VAS) pain score and Constant score.ResultsTwenty-nine patients were followed up from 12 months to 2 years, and the average follow-up time was 15.7 months. The pathological diagnosis of all patients after surgery was shoulder tuberculosis. No serious complications were found at the last follow-up, and the incision healed well. VAS pain score, Constant score, ESR, and CRP at the last follow-up were significantly improved compared with those before treatment (P< 0.05).ConclusionOn the basis of the standard use of anti-tuberculosis drugs before and after surgery, shoulder arthroscopy is used to treat early and mid-term shoulder tuberculosis, which can be diagnosed by direct observation under the arthroscope and postoperative pathological examination. It has the advantages of thorough lesion removal, minimal invasiveness, rapid recovery, and reliable clinical effect.
Continuous assessment of neuro-ventilatory drive during 12 h of pressure support ventilation in critically ill patients
Authors: Di mussi Rosa , Spadaro Savino , Volta Carlo Alberto , Bartolomeo Nicola , Trerotoli Paolo , Staffieri Francesco , Pisani Luigi , Iannuzziello Rachele , Dalfino Lidia , Murgolo Francesco , Grasso Salvatore 
Published: November 2020

AbstractIntroductionPressure support ventilation (PSV) should allow spontaneous breathing with a “normal” neuro-ventilatory drive. Low neuro-ventilatory drive puts the patient at risk of diaphragmatic atrophy while high neuro-ventilatory drive may causes dyspnea and patient self-inflicted lung injury. We continuously assessed for 12 h the electrical activity of the diaphragm (EAdi), a close surrogate of neuro-ventilatory drive, during PSV. Our aim was to document the EAdi trend and the occurrence of periods of “Low” and/or “High” neuro-ventilatory drive during clinical application of PSV.MethodIn 16 critically ill patients ventilated in the PSV mode for clinical reasons, inspiratory peak EAdi peak (EAdiPEAK), pressure time product of the trans-diaphragmatic pressure per breath and per minute (PTPDI/band PTPDI/min, respectively), breathing pattern and major asynchronies were continuously monitored for 12 h (from 8 a.m. to 8 p.m.). We identified breaths with “Normal” (EAdiPEAK5–15 μV), “Low” (EAdiPEAK < 5 μV) and “High” (EAdiPEAK > 15 μV) neuro-ventilatory drive.ResultsWithin all the analyzed breaths (177.117), the neuro-ventilatory drive, as expressed by the EAdiPEAK, was “Low” in 50.116 breath (28%), “Normal” in 88.419 breaths (50%) and “High” in 38.582 breaths (22%). The average times spent in “Low”, “Normal” and “High” class were 1.37, 3.67 and 0.55 h, respectively (p < 0.0001), with wide variations among patients. Eleven patients remained in the “Low” neuro-ventilatory drive class for more than 1 h, median 6.1 [3.9–8.5] h and 6 in the “High” neuro-ventilatory drive class, median 3.4 [2.2–7.8] h. The asynchrony index was significantly higher in the “Low” neuro-ventilatory class, mainly because of a higher number of missed efforts.ConclusionsWe observed wide variations in EAdi amplitude and unevenly distributed “Low” and “High” neuro ventilatory drive periods during 12 h of PSV in critically ill patients. Further studies are needed to assess the possible clinical implications of our physiological findings.
Continuous phase modulation with 1-bit quantization and oversampling using iterative detection and decoding
Authors: de Alencar Rodrigo R. M. , Landau Lukas T. N. , de Lamare Rodrigo C. 
Published: November 2020

AbstractA channel with continuous phase modulation and 1-bit ADC with oversampling is considered. Due to oversampling, higher-order modulations yield a higher achievable rate and this study presents methods to approach this with sophisticated channel coding. Iterative detection and decoding is considered, which exploits the soft information extracted from oversampled CPM sequences. Besides the strategy based on conventional channel coding techniques, a tailored bit mapping is proposed where two bits can be detected reliably at high SNR and only the third bit is affected by the uncertainties from the coarse quantization. With this, a convolutional code is only applied to the third bit. Our experiments with a turbo receiver show that the iterative detection and decoding is a promising approach for exploiting the additional information brought by oversampling. Moreover, it is shown that the proposed method based on the tailored bit mapping corresponds to a lower bit error rate in comparison with the case of conventional channel coding.
The analytical interface coupling of arbitrary-order fractional nonlinear hyperbolic scalar conservation laws
Authors: Shirkhorshidi S. M. R. , Othman W. A. M. , Awang M. A. Omar , Rostamy D. , Shirkhorshidi A. S. 
Published: November 2020

AbstractIn this paper, the existence and uniqueness of the interface coupling (IC) of time and spatial (TS) arbitrary-order fractional (AOF) nonlinear hyperbolic scalar conservation laws (NHSCL) are investigated. The technique of arbitrary fractional characteristic method (AFCM) is used to accomplish this task. We apply Jumarie’s modification of Riemann–Liouville and Liouville–Caputo’s definition to extend some formulae to the arbitrary-order fractional calculus. Then these formulae are utilized to prove the main theorem. In this process, we develop an analytic method, which gives us the ability to find the solution of IC AOF NHSCL. The feature of this method is that it enables us to verify that the obtained solution satisfies the fractional partial differential equation (FPDE), and the solution is unique. Furthermore, a few examples illustrate the implementation of this technique in the application section.
A note on the estimation of variance for big BAF sampling
Authors: Gove Jeffrey H. , Gregoire Timothy G. , Ducey Mark J. , Lynch Thomas B. 
Published: November 2020

AbstractBackgroundThe double sampling method known as “big BAF sampling” has been advocated as a way to reduce sampling effort while still maintaining a reasonably precise estimate of volume. A well-known method for variance determination, Bruce’s method, is customarily used because the volume estimator takes the form of a product of random variables. However, the genesis of Bruce’s method is not known to most foresters who use the method in practice.MethodsWe establish that the Taylor series approximation known as the Delta method provides a plausible explanation for the origins of Bruce’s method. Simulations were conducted on two different tree populations to ascertain the similarities of the Delta method to the exact variance of a product. Additionally, two alternative estimators for the variance of individual tree volume-basal area ratios, which are part of the estimation process, were compared within the overall variance estimation procedure.ResultsThe simulation results demonstrate that Bruce’s method provides a robust method for estimating the variance of inventories conducted with the big BAF method. The simulations also demonstrate that the variance of the mean volume-basal area ratios can be computed using either the usual sample variance of the mean or the ratio variance estimators with equal accuracy, which had not been shown previously for Big BAF sampling.ConclusionsA plausible explanation for the origins of Bruce’s method has been set forth both historically and mathematically in the Delta Method. In most settings, there is evidently no practical difference between applying the exact variance of a product or the Delta method—either can be used. A caution is articulated concerning the aggregation of tree-wise attributes into point-wise summaries in order to test the correlation between the two as a possible indicator of the need for further covariance augmentation.
Effects of interactions between variation in dopaminergic genes, traumatic life events, and anomalous self-experiences on psychosis proneness: Results from a cross-sectional study in a nonclinical sample
Authors: Frydecka Dorota , Kotowicz Kamila , Gawęda Łukasz , Prochwicz Katarzyna , Kłosowska Joanna , Rymaszewska Joanna , Samochowiec Agnieszka , Samochowiec Jerzy , Podwalski Piotr , Pawlak-Adamska Edyta , Szmida Elżbieta , Cechnicki Andrzej , Misiak Błażej 
Published: November 2020

Abstract

Background
There is a growing number of studies showing interactions between genetic polymorphisms associated with dopaminergic neurotransmission and traumatic life events (TLEs) on a risk of psychotic-like experiences (PLEs). Anomalous self-experiences (ASEs) have been associated both with TLEs as well as with PLEs. However, it remains unknown what is the role of ASEs in the complexity of gene–environment interactions on the emergence of PLEs.

Patients and methods
We included 445 young adults—university students from three big cities in Poland. We used the Traumatic Events Checklist to assess TLEs, the Inventory of Psychotic-Like anomalous self-experiences in order to measure ASEs, and the Prodromal Questionnaire (PQ16) to record the level of PLEs. The following gene polymorphisms, related to dopaminergic neurotransmission, were determined: the catechol-O-methyltransferase (COMT) rs4680 polymorphism, the dopamine D2 receptor (DRD2) rs6277 polymorphism, and the dopamine transporter 1 (DAT1) rs28363170 polymorphism.

Results
There was a significant effect of the interaction between the DAT1 polymorphism, a severity of ASEs, and a history of TLEs on the level of PLEs. Among the DAT1 10R/10R homozygotes with low level of ASEs, a severity of PLEs was significantly higher in individuals with a history of any TLEs. Higher scores of the PQ16 were associated with a greater severity of ASEs both in the DAT1 9R allele carriers and the DAT1 10R/10R homozygotes.

Conclusion
Our findings imply that genetic liability related to aberrant dopamine transport might impact the association between TLEs and PLEs in subjects with high levels of ASEs.