bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel LncRNA OXCT1-AS1 Indicates Poor Prognosis and Contributes to Tumorigenesis by Regulating miR-195/CDC25A Axis in Glioblastoma
Authors: Zhong Chen , Yu Qian , Zhou Shengjun , Peng Yucong , Liu Zhendong , Deng Yong , Guo Leiguang , Zhao Shiguang , Chen Gao 
Published: December 2020

Abstract
BackgroundIt’s well known that long noncoding RNAs (lncRNAs) contribute to multiple biological processes of human glioblastoma (GBM). However, identifying a specific lncRNA target is still the major difficulty. In this study, bioinformatics methods and competing endogenous RNA network (ceRNA) regulatory rules was used to identify GBM related lncRNAs, and found OXCT1 antisense RNA 1 (OXCT1-AS1) may acted as a potential therapeutic target for treatment of glioma.MethodsBased on the Gene Expression Omnibus (GEO) date set, we identified differential lncRNAs, microRNAs and mRNAs and constructed a lncRNA associated ceRNA network.Novel lncRNA OXCT1-AS1 was proposed to exercise its function as a ceRNA, and its potential targeted miRNAs was predicted through the database LncBase Predicted v.2. The expression pattern of OXCT1-AS1 was measured in glioma and normal tissue samples. Effect of OXCT1-AS1 on glioma cells were checked by cell count kit 8 assay, cell clone formation assay, transwell assay and flow cytometry in vitro, respectively. The dual-luciferase activity assay was performed to investigate the potential mechanism of ceRNA network. Finally, orthotopic mouse models of glioma was created to evaluate the influence of OXCT1-AS1 on tumor growth in vivo.ResultsIn this study, it was found that the expression of lncRNA OXCT1-AS1 is upregulated in both The Cancer Genome Atlas (TCGA) GBM cases and GBM tissue samples we collected, and a high expression of OXCT1-AS1 predicts poor prognosis of gliomas. Suppressing OXCT1-AS1 expression significantly decreased the proliferation of GBM cells and inhibited cell migration and invasion. We further investigated the potential mechanism and found OXCT1-AS1 may acted as a ceRNA of miR-195 to enhance CDC25A expression and attenuate glioma cells progression. Finally, knocking down of OXCT1-AS1 notably attenuated the severity of glioma in vivo.ConclusionOXCT1-AS1 inhibited glioma progression by regulating miR-195-5p/ CDC25A axis and can be a specific tumor marker and a novel potential therapeutic target for glioma treatment.
Prognostic Relevance of Elevated Plasma Osmolality on Admission in Acute Decompensated Heart Failure With Preserved Ejection Fraction: Insights From PURSUIT-HFpEF Registry
Authors: Nakagawa Akito , Yasumura Yoshio , Yoshida Chikako , Okumura Takahiro , Tateishi Jun , Yoshida Junichi , Tamaki Shunsuke , Yano Masamichi , Hayashi Takaharu , Nakagawa Yusuke , Yamada Takahisa , Nakatani Daisaku , Hikoso Shungo , Sakata Yasushi 
Published: December 2020

Abstract
BackgroundComplicated pathophysiology makes it difficult to identify the prognosis of heart failure with preserved ejection fraction (HFpEF). While plasma osmolality has been reported to have prognostic importance, mainly in heart failure with reduced ejection fraction (HFrEF), its prognostic meaning for HFpEF has not been elucidated. MethodsWe prospectively studied 960 patients in PURSUIT-HFpEF, a multicenter observational study of acute decompensated HFpEF inpatients. We divided patients into three groups according to the quantile values of plasma osmolality on admission. During a follow-up averaging 366 days, we examined the primary composite endpoint of cardiac mortality or heart failure re-admission using Kaplan-Meier curve analysis and Cox proportional hazard testing. Results216 (22.5%) patients reached the primary endpoint. Kaplan-Meier curve analysis revealed that the highest quantile of plasma osmolality on admission (higher than 300.3 mOsm/kg) was significantly associated with adverse outcomes (Log-rank P = 0.0095). Univariable analysis in the Cox proportional hazard model also revealed significantly higher rates of adverse outcomes in the higher plasma osmolality on admission (hazard ratio [HR] 7.29; 95% confidence interval [CI] 2.25–23.92, P = 0.0009). Multivariable analysis in the Cox proportional hazard model also showed that higher plasma osmolality on admission was significantly associated with adverse outcomes (HR 4.70; 95% CI 1.33–17.35, P = 0.0160) independently from other confounding factors such as age, gender, comorbid of atrial fibrillation, hypertension history, diabetes, malnutrition, and N-terminal pro-B-type natriuretic peptide elevation. ConclusionsHigher plasma osmolality on admission was prognostically important for acute decompensated HFpEF inpatients.
Renal dysfunction and prognosis of COVID-19 patients: a hospital-based retrospective cohort study
Authors: Xiang Hui-Xian , Xiang Ying , Fei Jun , Xu Zheng , Zheng Ling , Li Xiu-Yong , Fu Lin , Zhao Hui 
Published: December 2020

Abstract
Introduction: Increasing evidence indicate that coronavirus disease 2019 (COVID-19) is companied by renal dysfunction. However, the association of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2)-induced renal dysfunction with prognosis remains unclear.Materials and methods: This prospective case-cohort study analyzed 154 COVID-19 patients from the Second People’s Hospital of Fuyang City in Anhui Province. Clinical and demographic information were collected. Renal function was evaluated and its prognosis was followed up. Results: Of 154 hospitalized patients with COVID-19, 125 were common and 29 were severe patients. On admission, 16 (10.4%) patients were with renal dysfunction. Serum creatinine and cystatin C were increased, eGFR was decreased in severe patients compared with those in common patients. Renal dysfunction was more common in severe patients. By multivariate logistic regression, male, higher age and hypertension were three importantly independent risk factors of renal dysfunction in COVID-19 patients. Follow-up study found that at least one renal function marker of 3.33% patients remained abnormal in two weeks after discharge. Conclusion: Male elderly COVID-19 patients with hypertension elevates the risk of renal dysfunction. SARS-CoV-2-induced renal dysfunction are not fully recovered in two weeks after discharge.
CT-based classification systems for intra-articular calcaneal fractures: the inter- and intra-observer variations as well as integrality
Authors: wang zhongzheng , Liang Shaobo , Wang Yuchuan , Gao Ze , Tian Siyu , Zhao Kuo , Chen Wei , Hou Zhiyong , Zhang Yingze 
Published: December 2020

Abstract
Background: Several primary fracture classification systems (FCSs) have been widely used for intra-articular calcaneal fractures. The purpose of this study was to measure the inter- and intra-observer variations as well as integrality of the Zwipp, Crosby-Fitzgibbons, Sanders, and Eastwood-Atkins classification systems based on more accurate CT scans. Methods: 549 patients with intra-articular calcaneal fractures taken from a database in our level-I trauma centre (3 affiliated hospitals) were included from January 2018 to December 2019. For each case, normative CT (1 mm slices) scans were available. Four different observers reviewed all CT scans two times according to these 4 most prevalent FCSs within a 2-month interval. For these four FCSs, the kappa [κ] coefficient was used to evaluate interobserver reliability and intraobserver reproducibility, and the percentage that can be classified was used to indicate integrality. Results: The κ values were measured for Zwipp (κ= 0.38 interobserver, κ= 0.61 intraobserver), Crosby-Fitzgibbons (κ= 0.48 interobserver, κ= 0.79 intraobserver), Sanders (κ= 0.40 interobserver, κ= 0.57 intraobserver), and Eastwood-Atkins (κ= 0.44 interobserver, κ= 0.72 intraobserver). Furthermore, the integrality were calculated for Zwipp (100 %), Crosby-Fitzgibbons (100 %), Sanders (92 %) as well as Eastwood-Atkins (89.6 %). Conclusion: Compared with previous literatures, CT scanning with higher accuracy can significantly improve intraobserver reproducibility of Zwipp and Eastwood-Atkins FCSs, but it has no positive effect on variability of Sanders FCS and interobserver reliability of Crosby-Fitzgibbons FCS. While in terms of integrality, Zwipp and Crosby-Fitzgibbons FCSs appear to be superior to the other two FCSs.
The Ethylene Response factor SmERF1 Improves Salinity Tolerance and Impacts Seed Size in Tobacco
Authors: Hua Wenping , Chen Chen , Kong Weiwei , Wang Zhezhi 
Published: December 2020

Abstract
Background Ethylene response factor (ERF) proteins play vital roles in plant resistance and plant development. However, little is known about the ERF transcription factors of Salvia miltiorrhiza, which is a famous Chinese herb with good resistance to stress. Result Screened from our previous transcriptome data, SmERF1, an ERF transcript factor, was isolated from S. miltiorrhiza. SmERF1 had a single AP domain and was classified in the ERF E3 subfamily. SmERF1-expressing tobacco plants showed slower growth, less biomass and a decrease in chlorophyll only at the seedling stage, and there was no significant difference in other growth stages. In addition, seeds of tobacco plants with SmERF1 expression were smaller and lighter than those of wild plants, similar to some AP2 TFs. Under NaCl treatment, transgenic tobacco lines showed better tolerance to salinity, and the proline content, SOD and POD activities of transgenic lines were higher than those of wild-type plants, while MDA content was lower than that of wild-type plants. Furthermore, we determined the phytohormones related to plant resistance and showed that transgenic tobacco plants had higher ABA levels and lower GA levels than wild tobacco. The expression of SmERF1 regulated the expression of key enzyme genes related to plant hormone biosynthesis, such as NtSDR, NtGA20ox, NtACO and NtACS. Conclusions The SmERF1-expression in tobacco affect plant growth at seedling stage, and increase plant tolerance to salt. And the expression of SmERF1 cause tobacco seeds smaller and lighter. Our study suggested that SmERF1 enhanced tobacco tolerance to salt and impacted seed size through an ABA-dependent pathway.
Prediction and minimization of the heat treatment induced distortion in 8620H steel gear – Simulation and experimental verification
Authors: Shao Wen , Yi Mohan , Tang Jinyuan , Sun Siyuan 
Published: December 2020

Abstract
The considerable heat treatment induced runout value in the end face of the automobile main reducer gear is always dimensionally out of tolerance. It directly affects the dimensional accuracy, the grade of carburized and hardened gears, and the post-quenching manufacturing costs. In this study, three dimensional numerical models were developed to simulate the carburizing-quenching process of gear based on the multi-field coupling theory using DEFORM software. The results indicated that the ununiform cooling rate of the gear caused by the asymmetry of the web structure was the determinant of severe deformation of the gear. Therefore, a novel method was proposed to minimize the heat treatment induced runout value. It was found that the heat treatment induced runout value could be effectively controlled by the addition of a compensation ring and the support of a rod structure. Further experiments showed that the average runout value of the gear end face before and after the proposed heat treatment method were about 0.023 mm and 0.059 mm respectively, which was in good agreement with the simulated results. The novel approach proposed in this study led to a reduction of the gear runout value by 70.0%-76.9% compared to that of the original heat treatment process, which may serve as a practical and economical way to predict and minimize the heat treatment induced distortion in drive gear.
Alpinetin Inhibits RANKL-Induced Osteoclastogenesis and Ovariectomy-Induced Bone Loss by Modulating NFATc1 Transcription and Lysosomal Function
Authors: He Rongxin , Lu Jinwei , Chen Yazhou , Li Yong , Ye Chenyi , Hou Weiduo , Tang Lan , Wang Chenggui , Kuang Zhihui , Xiao Shining , Jiang Shuai , Chen Xi 
Published: December 2020

Abstract
BackgroundPostmenopausal osteoporosis is a chronic metabolic bone disease caused by excessive osteoclast activation, and osteoclasts are considered to be the sole participants in the degeneration and resorption of bone matrix for controlling bone integrity and continuity. The biological functions of osteoclasts depend critically on the number and activity of fused polykaryon. Hence, targeting osteoclast differentiation and activity can modulate bone resorption and alleviate osteoporosis. Alpinetin is widely used for excellent anti-inflammatory activities and little side-effect, but its role in osteoporosis remains unknown.ResultsIn this study, we investigated for the first time the ability of alpinetin to inhibit estrogen deficiency-induced bone loss. Alpinetin significantly reduced the expression levels of NFATc1 and its downstream genes, thereby inhibiting osteoclast differentiation in a concentration- and time-dependent manner. Additionally, alpinetin inhibited F-actin ring formation and bone resorption, as well as reduced the activation levels of NF-κB, ERK, and AKT signaling cascades. In mature osteoclasts, alpinetin remarkably inhibited integrin-mediated migration and lysosomal biogenesis and trafficking by modulating the PKCβ/TFEB and ATG5/LC3 axes. Importantly, alpinetin treatment in mice alleviated ovariectomy-induced bone volume loss. ConclusionOur findings strongly suggest that alpinetin plays a significant role in the regulation of NFATc1 production for the differentiation of osteoclasts and inhibits integrin-mediated cell migration and lysosomal function in mature osteoclasts, thus weaken the increased osteolytic ability due to estrogen deficiency. Alpinetin may represent a promising agent for the treatment of osteoporosis and other metabolic bone diseases.
Sensory Block Level Prediction of Spinal Anaesthesia With 0.5% Hyperbaric Bupivacaine:&nbsp;A Retrospective Study
Authors: Huang Yu-Yin , Chang Kuang-Yi 
Published: December 2020

Abstract
There is still no consensus on how to determine the dose of spinal anaesthesia with adequate sensory block for a planned surgery. This retrospective study aimed to explore the associations of miscellaneous factors with peak sensory block level after spinal anaesthesia with hyperbaric bupivacaine, and to construct a predictive model for single-shot spinal anaesthesia. We collected the records of 401 non-pregnant adults who underwent spinal anaesthesia with 0.5% hyperbaric bupivacaine at the L3-4 or L4-5 intervertebral space for lower body surgeries. Multiple linear regression analysis was used to investigate predictors of the block level and build up the predictive model. Five variables were identified as independent predictors of the peak sensory block level, including bupivacaine dose, height, weight, gender and age. The predictive model for peak block level after spinal anaesthesia could be expressed as a formula with these five variables and the estimated predictive power was 0.72. Based on this model, it is possible to determine a reasonable dose of hyperbaric bupivacaine for spinal anaesthesia, which gives adequate sensory block required for diverse surgical procedures in various patients and could be considered as a dose reference for sensory block height in spinal anaesthesia.
The Impact of China's Total Medical Insurance Package Payment Based on Compact County Medical Community and Its Implications for Medical Insurance Funds: Evidence from Y County, China
Authors: Liu Juming , Xu Yihua , Yang Yiqing , Luo Kaifu , Hong Yangyang , Hu Huaqin , Fan Menglin , Luo Yin , Xu Lai , Lu Heyun , Su Yan , Xiao Tianjie , Shi Ling , Xu Jiayan , Dong Qihua , Wang Jianxing 
Published: December 2020

Abstract
Background:Due to the irrational structure of medical and health resources, uneven distribution, and low level of grassroots services, traditional medical insurance payment methods in China’s underdeveloped areas have not been fully utilized in regulating medical service behaviors and guiding the allocation of medical resources. The medical insurance fund of many regions collapsed, the fund balance rate and compensation rate decreased year by year, and the medical expenses rose rapidly, which seriously affected the enthusiasm of the insured residents. As a results,the sustainable development of the medical insurance fund faced many difficulties and challenges.Based on this, we want to design a medical insurance policy suitable for underdeveloped areas in China to improve the capacity and efficiency of health services and maintain the stability of medical insurance funds.Methods:Collected medical insurance data of Y County in China from 2018 to 2019.The interruption time series(ITS) was used to evaluate the utilization of medical insurance funds in county-level hospitals, primary health service centers, and hospitals outside the medical community.Results:Inpatient expenditure(IE) per capita,out-of-pocket inpatient expenditure(OIE) per capita,and medical insurance expenditure (MIE)per capita in county-level hospitals increased by 84.682, 6.564 and 62.302 yuan per month respectively after the reform; Number of annul discharges persons(NOADP)from basic health service centers increased by 36.899 per month after the reform; IE per capita and MIE per capita increased by 18.624 yuan and 15.767 yuan per month on average after the reform; the residents hospitalized outside CCMC decreased by 9.545 per month on average after the reform. Conclusions:China should further implement the CCMC-based TMIPPM reform to control medical expenses and maintain the stability of medical insurance funds in in more trials.
Cryptolepine consolidates its anticancer property by increasing the anti-cancer pathway genes and decreasing that of pro-cancer pathway genes
Authors: Narkwa Patrick Williams , Domfeh Seth Agyei , Awandare Gordon , Mutocheluh Mohamed 
Published: December 2020

Abstract
Background: Cancers are one of the commonest causes of deaths globally . Reports indicate that greater than sixty percent of cancers in the world occur in low and middle-income countries with about seventy percent of all cancer deaths occurring in these regions. Conventional cancer treatments involve surgery, radiotherapy, chemotherapy, etc. However, the negative side effects such as high cost and toxicity associated with these treatment options have increased the demand for less toxic and less expensive anti-cancer drugs from natural sources. One of such natural products believed to have anti-cancer potential is cryptolepine, an alkaloid extracted from the roots of Western and Central African plant Cryptolepis sanguinolenta . In addition to its anti-cancer potential, cryptolepine has been reported to possess a myriad of pharmacological activities. However, the mechanisms underlying the anti-cancer and pharmacological activities of cryptolepine have not been fully explored. The current study sought to determine the mechanism underlying the anticancer properties of cryptolepine. Methods: We screened 45 immune and cancer signalling pathways for their regulation following treatment with cryptolepine using the dual-luciferase based Cignal Finder Multi-Pathway Reporter Arrays to pinpoint which pathways are regulated by cryptolepine. Additionally, the effects of cryptolepine on the transcript levels of interferon regulatory factor 1(IRF1), progesterone receptor (PR), hypoxia-inducible factor-1 alpha (HIF-1α) and signal transducer and activator of transcription 3 (STAT3) were assessed by real-time quantitative polymerase chain reaction. Results: We observed that of the 45 immune and cancer signalling pathways screened, nine were up-regulated while twenty-seven were down-regulated by cryptolepine. However, cryptolepine had no effect on nine of the pathways screened. We also observed that cryptolepine induced an increase in the transcript levels of IRF1 and PR but decreased that of HIF1-α and STAT3. Conclusion: The upregulation of human anti-cancer pathway genes including IRF-1 and PR and concomitant down-regulation of pro-cancer pathway genes including HIF1-α and STAT3 suggest additional mechanisms through which cryptolepine exhibits its anti-cancer activities.
Joint Association of Modifiable Lifestyle and Metabolic Health Status with Incidence of Cardiovascular Diabetes and All-Cause mortality: A Perspective Cohort Study
Authors: Zuo Yingting , Li Haibin , Chen Shuohua , Tian Xue , Mo Dapeng , Wu Shouling , Wang Anxin 
Published: December 2020

Abstract
Background We investigated the joint associations of modifiable lifestyle and metabolic factors with incident cardiovascular disease (CVD) and all-cause mortality.MethodsThis study included 94,831 participants (men, 79.76%; median age, 51.60 [43.47-58.87]) without a history of CVD at baseline from Kailuan study during 2006 to 2007 and followed them until new-onset CVD event, death or December 31, 2017. Baseline metabolic health status was assessed by Adult Treatment Panel-III (ATP-III) criteria and five lifestyle factors was collected using a self-reported questionnaire. We performed Cox proportional hazards models to evaluate the joint associations.Results During a median follow-up of 11.03 years, we observed 6,590 CVD events and 9,218 all-cause mortality. Participants within more metabolic risk components and least healthy lifestyle had the highest CVD risk (hazard ratio [HR] 2.06 [95% CI 1.77-2.39]) and mortality risk (HR 1.53 [95% CI 1.31-1.78]), as compared with the less metabolic risk components and most healthy lifestyle group. Compared with the most healthy lifestyle, the HR of CVD for participants with least healthy lifestyle was 1.26 (95% CI 1.17–1.37) in the category with low metabolic risk, 1.16 (95% CI 1.03–1.31) and 1.07 (95% CI 0.90–1.27) for those with medium and high metabolic risk, respectively.ConclusionsWe showed that healthy lifestyle and metabolic health were associated with a lower risk of CVD and all-cause mortality. The association between metabolic risk and the risk of CVD was not modified by healthy lifestyle. Our results indicated that healthy lifestyle should be promoted even for people with high metabolic risk.
The Application of Endoscopic Technique in High Cervical Anterior Approach to the Craniovertebral Junction
Authors: Liu Jiangtao , Ji Hongming , Zhang Gangli , Chen Shengli , Zhang Shiyuan , Zhao Jianwei , Chen Weiguo , Shang Xianghong , Hu Changchen 
Published: December 2020

Abstract
Objectives: Surgery on the craniovertebral junction (CVJ) presents particular challenges owing to the close proximity of critical neurovascular structures and the brainstem. It is difficult for classic approaches to obtain the extra exposure of neurovascular structures of the CVJ in practice.The surgical approach to the craniovertebral junction (CVJ) offers specific challenges. We explored the feasibility of an endoscope-assisted high anterior cervical approach to the CVJ. Methods: We quantitatively assessed the surgical corridor to, and extent of exposure of, the CVJ in six cadaveric specimens, using 0° and 30° endoscopes. Results: The endoscope provided sufficient exposure of neurovascular structures and the brainstem in the CVJ. Resection of the anterior arch of C1 was avoided in minimal anterior clivectomy. After removing the odontoid, greater exposure of the CVJ was obtained. Conclusion: An endoscope-assisted high anterior cervical approach to the CVJ preserves cervical spine stability while minimizing the risk of neurovascular injury within the surgical corridor.