bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolution of target and non-target based multiple herbicide resistance in a single Palmer amaranth (Amaranthus palmeri) population from Kansas
Authors: Chaudhari Sushila , Varanasi Vijay K. , Nakka Sridevi , Bhowmik Prasanta C. , Thompson Curtis R. , Peterson Dallas E. , Currie Randall S. , Jugulam Mithila 
Published: June 2020

AbstractThe evolution of resistance to multiple herbicides in Palmer amaranth is a major challenge for its management. In this study, a Palmer amaranth population from Hutchinson, Kansas (HMR), was characterized for resistance to inhibitors of photosystem II (PSII) (e.g., atrazine), acetolactate synthase (ALS) (e.g., chlorsulfuron), and EPSP synthase (EPSPS) (e.g., glyphosate), and this resistance was investigated. About 100 HMR plants were treated with field-recommended doses (1×) of atrazine, chlorsulfuron, and glyphosate, separately along with Hutchinson multiple-herbicide (atrazine, chlorsulfuron, and glyphosate)–susceptible (HMS) Palmer amaranth as control. The mechanism of resistance to these herbicides was investigated by sequencing or amplifying the psbA, ALS, and EPSPS genes, the molecular targets of atrazine, chlorsulfuron, and glyphosate, respectively. Fifty-two percent of plants survived a 1× (2,240 g ai ha−1) atrazine application with no known psbA gene mutation, indicating the predominance of a non–target site resistance mechanism to this herbicide. Forty-two percent of plants survived a 1× (18 g ai ha−1) dose of chlorsulfuron with proline197serine, proline197threonine, proline197alanine, and proline197asparagine, or tryptophan574leucine mutations in the ALS gene. About 40% of the plants survived a 1× (840 g ae ha−1) dose of glyphosate with no known mutations in the EPSPS gene. Quantitative PCR results revealed increased EPSPS copy number (50 to 140) as the mechanism of glyphosate resistance in the survivors. The most important finding of this study was the evolution of resistance to at least two sites of action (SOAs) (~50% of plants) and to all three herbicides due to target site as well as non–target site mechanisms. The high incidence of individual plants with resistance to multiple SOAs poses a challenge for effective management of this weed.
Treatment of bypass failure in patients with chronic limb threatening ischemia – open surgery vs. percutaneous mechanical thrombectomy
Authors: Migliara Bruno , Cappellari Tania Francesca , Mirandola Mattia , Griso Andrea , Kolasa Katarzyna , Zah Vladimir , Nicoletti Cristian , Lino Marcello 
Published: June 2020

Summary: Background: Lower limb bypass occlusion in patients with chronic limb threating ischemia remains a challenge. We can choose between different treatment options: open surgery, local thrombolysis, thrombectomy/atherectomy devices. In this pilot study, we compare clinical outcomes and treatment costs between open surgery (OS) and percutaneous mechanical thrombectomy (pMTH). Patients and methods: This pilot study represents a retrospective analysis of hospital data of 48 occluded bypasses admitted from 2013 to 2018. Only patients presenting with severe ischemia and recrudescence of symptoms (Rutherford 4–6) were included in the current analysis. Two cohorts of patients were analysed: patients who underwent OS and patients that underwent pMTH. Primary clinical outcomes were one-year cumulative patency and limb salvage rates. Total cost was calculated as a sum of intra- and post-operative costs. To weigh clinical benefits against the economic consequences of OS versus pMTH a cost-effectiveness framework was adopted. Results: We analysed a series of 48 occluded bypasses 17 treated with open surgery and 31 with pMTH. Procedural success was 100% in both groups. When comparing one-year death rates ( p-value = .22) and re-occlusion rates ( p-value = .43), no statistically significant differences were observed between the two cohorts. Mean patency duration in the surgery cohort was significantly shorter ( p-value < .05). Primary patency (OS 41.2% vs. pMTH 48.4%) and limb salvage rate (OS 88.2% vs. pMTH 90.3%) at one year are similar in both groups. The total cost of surgery was substantially higher (OS 10,159€ vs. pMTH 8,401€) Conclusions: This pilot study, although limited to 48 occluded bypasses, demonstrates that endovascular treatment with pMTH is less invasive, less time consuming and less expensive, and produces greater health benefits than traditional OS.
Unprecedented Drought Challenges for Texas Water Resources in a Changing Climate: What Do Researchers and Stakeholders Need to Know?
Authors: Nielsen‐Gammon John W. , Banner Jay L. , Cook Benjamin I. , Tremaine Darrel M. , Wong Corinne I. , Mace Robert E. , Gao Huilin , Yang Zong‐Liang , Gonzalez Marisa Flores , Hoffpauir Richard , Gooch Tom , Kloesel Kevin 
Published: June 2020

AbstractLong‐range water planning is complicated by factors that are rapidly changing in the 21st century, including climate, population, and water use. Here, we analyze climate factors and drought projections for Texas as an example of a diverse society straddling an aridity gradient to examine how the projections can best serve water stakeholder needs. We find that climate models are robust in projecting drying of summer‐season soil moisture and decreasing reservoir supplies for both the eastern and western portions of Texas during the 21st century. Further, projections indicate drier conditions during the latter half of the 21st century than even the most arid centuries of the last 1,000 years that included megadroughts. To illustrate how accounting for drought nonstationarity may increase water resiliency, we consider generalized case studies involving four key stakeholder groups: agricultural producers, large surface water suppliers, small groundwater management districts, and regional water planning districts. We also examine an example of customized climate information being used as input to long‐range water planning. We find that while stakeholders value the quantitative capability of climate model outputs, more specific climate‐related information better supports resilience planning across multiple stakeholder groups. New suites of tools could provide necessary capacity for both short‐ and long‐term, stakeholder‐specific adaptive planning.
NAC2H: The North American Climate Change and Hydroclimatology Data Set
Authors: Arsenault Richard , Brissette François , Chen Jie , Guo Qiang , Dallaire Gabrielle 
Published: June 2020

AbstractA data set containing hydrometeorological and hydroclimatological data for 3,540 watersheds in North America is described. The data set contains four main parts: (a) observed hydrometeorological data including daily streamflow observations, precipitation, minimum temperature, and maximum temperature; (b) 20 bias‐corrected climate model projections for two Representative Concentration Pathway (RCP) scenarios and five bias correction methods; (c) hydrological model calibration parameters and simulated streamflow for 4 hydrological models, 2 objective functions, and 10 calibration parameter sets for the reference period; and (d) hydrological simulations for each of the combinations of the abovementioned elements of the climate change impact study chain, for a total of 16,000 combinations. The data set also contains simulations and bias‐corrected climate for 30‐year horizons corresponding to 1.5°C and 2°C temperature increases for a subset of the climate models, for an additional 8,000 combinations. All simulations in the reference period are also provided. Fifty‐one precomputed hydrological indices are made available for each simulation. Overall, 2.89 × 1012 years of simulations are classified, analyzed, compressed, and made available for all researchers. This data set can be used to evaluate the uncertainty of various components in the impact study chain, to establish relationships between catchment properties and hydrological response to climate change, and to evaluate the spatial distribution of hydrological change according to a multitude of hydrological indices.
Assessing the Steady‐State Assumption in Water Balance Calculation Across Global Catchments
Authors: Han Juntai , Yang Yuting , Roderick Michael L. , McVicar Tim R. , Yang Dawen , Zhang Shulei , Beck Hylke E. 
Published: June 2020

AbstractIt has long been assumed that over a sufficiently long period of time, changes in catchment water storage (ΔS) are a relatively minor term compared to other fluxes and can be neglected in the catchment water balance equation. However, the validity of this fundamental assumption has rarely been tested, and the associated uncertainties in water balance calculations remain unknown. Here, we use long‐term (1982–2011) observations of monthly streamflow (Q) and precipitation (P) for 1,057 global unimpaired catchments, combined with four independent evapotranspiration (E) estimates to infer ΔS and to provide a global assessment of the steady‐state assumption in catchment water balance calculations. Results show that when the threshold for steady state is set to 5% of the mean monthly P, ~70% of the catchments attain steady state within 10 years while ~6% of the catchments fail to reach a steady state even after 30 years. The time needed for a catchment to reach steady state (τs) shows a close relationship with climatic aridity and vegetation coverage, with arid/semiarid and sparsely vegetated catchments generally having a longer τs. Additionally, increasing snowfall fraction also increases τs. The imbalance (ewb) caused by ignoring ΔS decreases as averaging period for water balance calculations increases as expected. For a typical 10‐year averaging period, ewb accounts for ~7% of P in arid, but that decreases to ~3% of P in humid catchments. These results suggest that catchment properties should be considered when applying the steady‐state assumption and call for caution when ignoring ΔS in arid/semiarid regions.
A rasterized building footprint dataset for the United States
Authors: Heris Mehdi P. , Foks Nathan Leon , Bagstad Kenneth J. , Troy Austin , Ancona Zachary H. 
Published: June 2020

AbstractMicrosoft released a U.S.-wide vector building dataset in 2018. Although the vector building layers provide relatively accurate geometries, their use in large-extent geospatial analysis comes at a high computational cost. We used High-Performance Computing (HPC) to develop an algorithm that calculates six summary values for each cell in a raster representation of each U.S. state, excluding Alaska and Hawaii: (1) total footprint coverage, (2) number of unique buildings intersecting each cell, (3) number of building centroids falling inside each cell, and area of the (4) average, (5) smallest, and (6) largest area of buildings that intersect each cell. These values are represented as raster layers with 30 m cell size covering the 48 conterminous states. We also identify errors in the original building dataset. We evaluate precision and recall in the data for three large U.S. urban areas. Precision is high and comparable to results reported by Microsoft while recall is high for buildings with footprints larger than 200 m2 but lower for progressively smaller buildings.
Functional kleptoplasts intermediate incorporation of carbon and nitrogen in cells of the Sacoglossa sea slug Elysia viridis
Authors: Cruz Sónia , LeKieffre Charlotte , Cartaxana Paulo , Hubas Cédric , Thiney Najet , Jakobsen Sofie , Escrig Stéphane , Jesus Bruno , Kühl Michael , Calado Ricardo , Meibom Anders 
Published: June 2020

AbstractSome sacoglossan sea slugs incorporate intracellular functional algal chloroplasts, a process termed kleptoplasty. “Stolen” chloroplasts (kleptoplasts) can remain photosynthetically active up to several months, contributing to animal nutrition. Whether this contribution occurs by means of translocation of photosynthesis-derived metabolites from functional kleptoplasts to the animal host or by simple digestion of such organelles remains controversial. Imaging of 13C and 15N assimilation over a 12-h incubation period of Elysia viridis sea slugs showed a light-dependent incorporation of carbon and nitrogen, observed first in digestive tubules and followed by a rapid accumulation into chloroplast-free organs. Furthermore, this work revealed the presence of 13C-labeled long-chain fatty acids (FA) typical of marine invertebrates, such as arachidonic (20:4n-6) and adrenic (22:4n-6) acids. The time frame and level of 13C- and 15N-labeling in chloroplast-free organs indicate that photosynthesis-derived primary metabolites were made available to the host through functional kleptoplasts. The presence of specific 13C-labeled long-chain FA, absent from E. viridis algal food, indicates animal based-elongation using kleptoplast-derived FA precursors. Finally, carbon and nitrogen were incorporated in organs and tissues involved in reproductive functions (albumin gland and gonadal follicles), implying a putative role of kleptoplast photosynthesis in the reproductive fitness of the animal host.
Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings
Authors: Kedzierska Anna Ewa , Lorek Daria , Slawek Anna , Chelmonska-Soyta Anna 
Published: June 2020

AbstractThe imbalance in immune tolerance may cause the variety of reproductive failures. An intravenous immunoglobulin infusion (IVIg) therapy is used to improve the live birth rate in women suffering from recurrent pregnancy loss, recurrent spontaneous abortions and recurrent implantation failures. However, the results of IVIg studies are still inconclusive as IVIg infusion in women suffering from pregnancy loss is sometimes ineffective. One of the mechanisms of action of this treatment is inhibition of B cells differentiation and expansion of Tregs and secretion of interleukin 10. It was proposed that immunomodulatory effects of IVIg may be attributed to tregitopes - self-IgG-derived epitopes present in the structure of immunoglobulins. Similarly to IVIg, tregitopes cause the expansion of Tregs and secretion of antigen-specific effector cytokine response. Here, we studied whether the administration of mouse tregitope 167 and/or 289 can prevent abortions in mouse abortion-prone mouse matings. We revealed that tregitopes reduce the foetal death rate. This may be driven by observed higher pool of peripheral Tregs, increased production of IL-10 by Tregs and Bregs and/or maintaining the tolerogenic phenotype of antigen-presenting cells. We believe that our findings may indicate a potential alternative to IVIg for therapeutic intervention in case of pregnancy failures.
Age and Sex Influences Gamma-aminobutyric Acid Concentrations in the Developing Brain of Very Premature Infants
Authors: Basu Sudeepta K. , Pradhan Subechhya , Jacobs Marni B. , Said Mariam , Kapse Kushal , Murnick Jonathan , Whitehead Matthew T. , Chang Taeun , du Plessis Adre J. , Limperopoulos Catherine 
Published: June 2020

AbstractGamma-aminobutyric acid (GABA) and glutamate are principal neurotransmitters essential for late gestational brain development and may play an important role in prematurity-related brain injury. In vivo investigation of GABA in the preterm infant with standard proton magnetic resonance spectroscopy (1H-MRS) has been limited due to its low concentrations in the developing brain, and overlap in the spectrum by other dominant metabolites. We describe early postnatal profiles of in vivo GABA and glutamate concentrations in the developing preterm brain measured by using the J-difference editing technique, Mescher-Garwood point resolved spectroscopy. We prospectively enrolled very preterm infants born ≤32 weeks gestational age and non-sedated 1H-MRS (echo time 68 ms, relaxation time 2000 ms, 256 signal averages) was acquired on a 3 Tesla magnetic resonance imaging scanner from a right frontal lobe voxel. Concentrations of GABA + (with macromolecules) was measured from the J-difference spectra; whereas glutamate and composite glutamate + glutamine (Glx) were measured from the unedited (OFF) spectra and reported in institutional units. We acquired 42 reliable spectra from 38 preterm infants without structural brain injury [median gestational age at birth of 28.0 (IQR 26.0, 28.9) weeks; 19 males (50%)] at a median postmenstrual age of 38.4 (range 33.4 to 46.4) weeks. With advancing post-menstrual age, the concentrations of glutamate OFF increased significantly, adjusted for co-variates (generalized estimating equation β = 0.22, p = 0.02). Advancing postnatal weeks of life at the time of imaging positively correlated with GABA + (β = 0.06, p = 0.02), glutamate OFF (β = 0.11, p = 0.02) and Glx OFF (β = 0.12, p = 0.04). Male infants had higher GABA + (1.66 ± 0.07 vs. 1.33 ± 0.11, p = 0.01) concentrations compared with female infants. For the first time, we report the early ex-utero developmental profile of in vivo GABA and glutamate stratified by age and sex in the developing brain of very preterm infants. This data may provide novel insights into the pathophysiology of neurodevelopmental disabilities reported in preterm infants even in the absence of structural brain injury.
Antioxidants help favorably regulate the kinetics of lipid peroxidation, polyunsaturated fatty acids degradation and acidic cannabinoids decarboxylation in hempseed oil
Authors: Pratap Singh Anubhav , Fathordoobady Farahnaz , Guo Yigong , Singh Anika , Kitts David D. 
Published: June 2020

AbstractThe seed of the hemp plant (Cannabis sativa L.) has been revered as a nutritional resource in Old World Cultures. This has been confirmed by contemporary science wherein hempseed oil (HSO) was found to exhibit a desirable ratio of omega-6 and omega-3 polyunsaturated fatty acids (PUFAs) considered optimal for human nutrition. HSO also contains gamma-linoleic acid (GLA) and non-psychoactive cannabinoids, which further contribute to its’ potential bioactive properties. Herein, we present the kinetics of the thermal stability of these nutraceutical compounds in HSO, in the presence of various antioxidants (e.g. butylated hydroxytoluene, alpha-tocopherol, and ascorbyl palmitate). We focussed on oxidative changes in fatty acid profile and acidic cannabinoid stability when HSO was heated at different temperatures (25 °C to 85 °C) for upto 24 h. The fatty acid composition was evaluated using both GC/MS and 1H-NMR, and the cannabinoids profile of HSO was obtained using both HPLC-UV and HPLC/MS methods. The predicted half-life (DT50) for omega-6 and omega-3 PUFAs in HSO at 25 °C was about 3 and 5 days, respectively; while that at 85 °C was about 7 and 5 hours respectively, with respective activation energies (Ea) being 54.78 ± 2.36 and 45.02 ± 2.87 kJ/mol. Analysis of the conjugated diene hydroperoxides (CDH) and p-Anisidine value (p-AV) revealed that the addition of antioxidants significantly (p < 0.05) limited lipid peroxidation of HSO in samples incubated at 25–85 °C for 24 h. Antioxidants reduced the degradation constant (k) of PUFAs in HSO by upto 79%. This corresponded to a significant (p < 0.05) increase in color stability and pigment retention (chlorophyll a, chlorophyll b and carotenoids) of heated HSO. Regarding the decarboxylation kinetics of cannabidiolic acid (CBDA) in HSO, at both 70 °C and 85 °C, CBDA decarboxylation led to predominantly cannabidiol (CBD) production. The half-life of CBDA decarboxylation (originally 4 days) could be increased to about 17 days using tocopherol as an antioxidant. We propose that determining acidic cannabinoids decarboxylation kinetics is a useful marker to measure the shelf-life of HSO. The results from the study will be useful for researchers looking into the thermal treatment of hempseed oil as a functional food product, and those interested in the decarboxylation kinetics of the acidic cannabinoids.
Cavityless self-organization of ultracold atoms due to the feedback-induced phase transition
Authors: Ivanov Denis A. , Ivanova Tatiana Yu. , Caballero-Benitez Santiago F. , Mekhov Igor B. 
Published: June 2020

AbstractFeedback is a general idea of modifying system behavior depending on the measurement outcomes. It spreads from natural sciences, engineering, and artificial intelligence to contemporary classical and rock music. Recently, feedback has been suggested as a tool to induce phase transitions beyond the dissipative ones and tune their universality class. Here, we propose and theoretically investigate a system possessing such a feedback-induced phase transition. The system contains a Bose-Einstein condensate placed in an optical potential with the depth that is feedback-controlled according to the intensity of the Bragg-reflected probe light. We show that there is a critical value of the feedback gain where the uniform gas distribution loses its stability and the ordered periodic density distribution emerges. Due to the external feedback, the presence of a cavity is not necessary for this type of atomic self-organization. We analyze the dynamics after a sudden change of the feedback control parameter. The feedback time constant is shown to determine the relaxation above the critical point. We show as well that the control algorithm with the derivative of the measured signal dramatically decreases the transient time.
Fourier analysis of circumpapillary retinal nerve fiber layer thickness in optical coherence tomography for differentiating myopia and glaucoma
Authors: Hsieh Ming-Hung , Chang Yu-Fan , Liu Catherine Jui-Ling , Ko Yu-Chieh 
Published: June 2020

AbstractDifferentiating glaucoma from myopic eye is a challenge to ophthalmologists. We try to develop a new discrete Fourier transform (DFT) model for analyzing optical coherence tomography data for the circumpapillary retinal nerve fiber layer (cpRNFL), and investigate DFT as a new diagnostic tool for glaucomatous myopic eyes. The thicknesses of 12 equidistant cpRNFL points were transformed into 6 signals in the frequency domain, ranging from 1 to 6 Hz. In all 232 eyes, generalized linear model showed that 1 Hz, 2 Hz, and 4 Hz were associated with glaucoma, high myopia, and the interaction between glaucoma and high myopia. The 3 Hz signal was associated with glaucoma and high myopia exclusively. A receiver operating characteristic curve analysis of the 3 Hz signals showed areas under the curves of 0.93 (95% CI 0.90–0.96) and 0.93 (95% CI 0.88–0.98), for diagnosing glaucoma in all subjects and in the highly myopic group, respectively. The DFT model is useful to differentiate glaucoma from non-glaucomatous change and showed potential as a diagnostic tool for glaucomatous myopic eyes.
Cerebral autoregulation and response to intravenous thrombolysis for acute ischemic stroke
Authors: Nogueira Ricardo C. , Lam Man Y. , Llwyd Osian , Salinet Angela S. M. , Bor-Seng-Shu Edson , Panerai Ronney B. , Robinson Thompson G. 
Published: June 2020

AbstractWe hypothesized that knowledge of cerebral autoregulation (CA) status during recanalization therapies could guide further studies aimed at neuroprotection targeting penumbral tissue, especially in patients that do not respond to therapy. Thus, we assessed CA status of patients with acute ischemic stroke (AIS) during intravenous r-tPA therapy and associated CA with response to therapy. AIS patients eligible for intravenous r-tPA therapy were recruited. Cerebral blood flow velocities (transcranial Doppler) from middle cerebral artery and blood pressure (Finometer) were recorded to calculate the autoregulation index (ARI, as surrogate for CA). National Institute of Health Stroke Score was assessed and used to define responders to therapy (improvement of ≥ 4 points on NIHSS measured 24–48 h after therapy). CA was considered impaired if ARI < 4. In 38 patients studied, compared to responders, non-responders had significantly lower ARI values (affected hemisphere: 5.0 vs. 3.6; unaffected hemisphere: 5.4 vs. 4.4, p = 0.03) and more likely to have impaired CA (32% vs. 62%, p = 0.02) during thrombolysis. In conclusion, CA during thrombolysis was impaired in patients who did not respond to therapy. This variable should be investigated as a predictor of the response to therapy and to subsequent neurological outcome.