bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imaging transmembrane dynamics of biomolecules at live cell plasma membranes using quenchers in extracellular environment
Authors: Hou Wenqing , Ma Dongfei , He Xiaolong , Han Weijing , Ma Jianbing , Xu Chunhua , Xie Ruipei , Fan Qi-hui , Ye Fangfu , Hu Shuxin , Lu Ying , Li Ming 
Published: August 2020

AbstractIt is a big challenge to measure position changes of biomolecules in the direction normal to the plasma membranes of living cells. We developed a one donor-multiple quenchers Fӧrster resonance energy transfer method by using non-fluorescent quenchers in the extracellular environment. It senses subnanometer position changes of a fluorophore-labeled biomolecule in the plasma membrane. The method was validated by monitoring flip-flops of individual lipid molecules incorporated in plasma membranes. We studies membrane perforation by a host defense peptide from the extracellular side and found that the pore-forming peptide is dynamic, switching among different insertion depths. The method is especially useful in studying interactions of membrane proteins with the inner surfaces of plasma membranes. Our method will find wide applications in systematic analysis of fundamental cellular processes at plasma membranes.
Estimating the impact of differential adherence on the comparative effectiveness of stool-based colorectal cancer screening using the CRC-AIM microsimulation model
Authors: Piscitello Andrew , Saoud Leila , Fendrick A. Mark , Borah Bijan J. , Lich Kristen Hassmiller , Matney Michael , Ozbay A. Burak , Parton Marcus , Limburg Paul J. 
Published: August 2020

AbstractBackgroundReal-world adherence to colorectal cancer (CRC) screening strategies is imperfect. The CRC-AIM microsimulation model was used to estimate the impact of imperfect adherence on the relative benefits and burdens of guideline-endorsed, stool-based screening strategies.MethodsPredicted outcomes of multi-target stool DNA (mt-sDNA), fecal immunochemical tests (FIT), and high-sensitivity guaiac-based fecal occult blood tests (HSgFOBT) were simulated for 40-year-olds free of diagnosed CRC. For robustness, imperfect adherence was incorporated in multiple ways and with extensive sensitivity analysis. Analysis 1 assumed adherence from 0%-100%, in 10% increments. Analysis 2 longitudinally applied real-world first-round differential adherence rates (base-case imperfect rates=40% annual FIT vs 34% annual HSgFOBT vs 70% triennial mt-sDNA). Analysis 3 randomly assigned individuals to receive 1, 5, or 9 lifetime (9=100% adherence) mt-sDNA tests and 1, 5, or 9 to 26 (26=100% adherence) FIT tests. Outcomes are reported per 1000 individuals compared with no screening.ResultsEach screening strategy decreased CRC incidence and mortality versus no screening. In individuals screened between ages 50-75 and adherence ranging from 10%-100%, the life-years gained (LYG) for triennial mt-sDNA ranged from 133.1-300.0, for annual FIT from 96.3-318.1, and for annual HSgFOBT from 99.8-320.6. At base-case imperfect adherence rates, mt-sDNA resulted in 19.1% more LYG versus FIT, 25.4% more LYG versus HSgFOBT, and generally had preferable efficiency ratios while offering the most LYG. Completion of at least 21 FIT tests is needed to reach approximately the same LYG achieved with 9 mt-sDNA tests.ConclusionsAdherence assumptions affect the conclusions of CRC screening microsimulations that are used to inform CRC screening guidelines. LYG from FIT and HSgFOBT are more sensitive to changes in adherence assumptions than mt-sDNA because they require more tests be completed for equivalent benefit. At imperfect adherence rates, mt-sDNA provides more LYG than FIT or HSgFOBT at an acceptable tradeoff in screening burden.
The global landscape of SARS-CoV-2 genomes, variants, and haplotypes in 2019nCoVR
Authors: Song Shuhui , Ma Lina , Zou Dong , Tian Dongmei , Li Cuiping , Zhu Junwei , Chen Meili , Wang Anke , Ma Yingke , Li Mengwei , Teng Xufei , Cui Ying , Duan Guangya , Zhang Mochen , Jin Tong , Shi Chengmin , Du Zhenglin , Zhang Yadong , Liu Chuandong , Li Rujiao , Zeng Jingyao , Hao Lili , Jiang Shuai , Chen Hua , Han Dali , Xiao Jingfa , Zhang Zhang , Zhao Wenming , Xue Yongbiao , Bao Yiming 
Published: August 2020

AbstractOn 22 January 2020, the National Genomics Data Center (NGDC), part of the China National Center for Bioinformation (CNCB), created the 2019 Novel Coronavirus Resource (2019nCoVR), an open-access SARS-CoV-2 information resource. 2019nCoVR features a comprehensive integration of sequence and clinical information for all publicly available SARS-CoV-2 isolates, which are manually curated with value-added annotations and quality evaluated by our in-house automated pipeline. Of particular note, 2019nCoVR performs systematic analyses to generate a dynamic landscape of SARS-CoV-2 genomic variations at a global scale. It provides all identified variants and detailed statistics for each virus isolate, and congregates the quality score, functional annotation, and population frequency for each variant. It also generates visualization of the spatiotemporal change for each variant and yields historical viral haplotype network maps for the course of the outbreak from all complete and high-quality genomes. Moreover, 2019nCoVR provides a full collection of SARS-CoV-2 relevant literature on COVID-19 (Coronavirus Disease 2019), including published papers from PubMed as well as preprints from services such as bioRxiv and medRxiv through Europe PMC. Furthermore, by linking with relevant databases in CNCB-NGDC, 2019nCoVR offers data submission services for raw sequence reads and assembled genomes, and data sharing with National Center for Biotechnology Information. Collectively, all SARS-CoV-2 genome sequences, variants, haplotypes and literature are updated daily to provide timely information, making 2019nCoVR a valuable resource for the global research community. 2019nCoVR is accessible athttps://bigd.big.ac.cn/ncov/.
Formation and synaptic control of active transient working memory representations
Authors: Becker Sophia , Nold Andreas , Tchumatchenko Tatjana 
Published: August 2020

AbstractNeural representations of working memory maintain information temporarily and make it accessible for processing. This is most feasible in active, spiking representations. State-of-the-art modeling frameworks, however, reproduce working memory representations that are either transient but non-active or active but non-transient. Here, we analyze a biologically motivated working memory model which shows that synaptic short-term plasticity and noise emerging from spiking networks can jointly produce a working memory representation that is both active and transient. We investigate the effect of a synaptic signaling mechanism whose dysregulation is related to schizophrenia and show how it controls transient working memory duration through presynaptic, astrocytic and postsynaptic elements. Our findings shed light on the computational capabilities of healthy working memory function and offer a possible mechanistic explanation for how molecular alterations observed in psychiatric diseases such as schizophrenia can lead to working memory impairments.
Distinct effects of different matrix proteoglycans on collagen fibrillogenesis and cell-mediated collagen reorganization
Authors: Chen Dongning , Smith Lucas R. , Khandekar Gauri , Patel Pavan , Yu Christopher K. , Zhang Kehan , Chen Christopher S. , Han Lin , Wells Rebecca G. 
Published: August 2020

AbstractThe extracellular matrix (ECM) is a complex mixture composed of fibrillar collagens as well as additional protein and carbohydrate components. Proteoglycans (PGs) contribute to the heterogeneity of the ECM and play an important role in its structure and function. While the small leucine rich proteoglycans (SLRPs), including decorin and lumican, have been studied extensively as mediators of collagen fibrillogenesis and organization, the function of large matrix PGs in collagen matrices is less well known. In this study, we showed that different matrix PGs have distinct roles in regulating collagen behaviors. We found that versican, a large chondroitin sulfate PG, promotes collagen fibrillogenesis in a turbidity assay and upregulates cell-mediated collagen compaction and reorganization, whereas aggrecan, a structurally-similar large PG, has different and often opposing effects on collagen. Compared to versican, decorin and lumican also have distinct functions in regulating collagen behaviors. The different ways in which matrix PGs interact with collagen have important implications for understanding the role of the ECM in diseases such as fibrosis and cancer, and suggest that matrix PGs are potential therapeutic targets.HighlightsSmall leucine rich proteoglycans (SLRPs) and large chondroitin sulfate (CS) proteoglycans (PGs) have distinct effects on collagen fibrous network behavior.Unlike other matrix proteoglycans, versican promotes collagen fibrillogenesis in anin vitrospectrophotometric (turbidity) assay.The versican core protein has a larger impact on collagen behavior in a fibrillogenesis assay than its glycosaminoglycan chains do.Versican increases the diameter of collagen fibers and the porosity of collagen fibrous networks, unlike aggrecan and SLRPs.The addition of versican to collagen does not alter fibroblast contractility but leads to enhanced cell-mediated collagen reorganization and contraction.
Representational Dynamics Preceding Conscious Access
Authors: Alilović Josipa , van Moorselaar Dirk , Graetz Marcel , van Gaal Simon , Slagter Heleen A. 
Published: August 2020

AbstractOur senses are continuously bombarded with more information than our brain can process up to the level of awareness. The present study aimed to enhance understanding on how attentional selection shapes conscious access under conditions of rapidly changing input. Using an attention task, EEG, and multivariate decoding of individual target- and distractor-defining features, we specifically examined dynamic changes in the representation of targets and distractors as a function of conscious access and the task-relevance (target or distractor) of the preceding item in the RSVP stream. At the behavioral level, replicating previous work and suggestive of a flexible gating mechanism, we found a significant impairment in conscious access to targets (T2) that were preceded by a target (T1) followed by one or two distractors (i.e., the attentional blink), but striking facilitation of conscious access to targets shown directly after another target (i.e., lag-1 sparing and blink reversal). At the neural level, conscious access to T2 was associated with enhanced early- and late-stage T1 representations and enhanced late-stage D1 representations, and interestingly, could be predicted based on the pattern of EEG activation well before T1 was presented. Yet, across task conditions, we did not find convincing evidence for the notion that conscious access is affected by rapid top-down selection-related modulations of the strength of early sensory representations induced by the preceding visual event. These results cannot easily be explained by existing accounts of how attentional selection shapes conscious access under rapidly changing input conditions, and have important implications for theories of the attentional blink and consciousness more generally.
mRNA translation control by Dhx36 binding to 5’UTR G-quadruplex structures is essential for skeletal muscle stem cell regenerative functions
Authors: Chen Xiaona , Yuan Jie , Xue Guang , Sanz Silvia Campanario , Wang Di , Wang Wen , Mou Xi , Umar Mubarak Ishaq , Isern Joan , Zhao Yu , He Liangqiang , Li Yuying , Mann Christopher J. , Yu Xiaohua , Wang Lei , Perdiguero Eusebio , Chen Wei , Xue Yuanchao , Nagamine Yoshikuni , Kwok Chun-Kit , Sun Hao , Muñoz-Cánoves Pura , Wang Huating 
Published: August 2020

AbstractSkeletal muscle has a remarkable ability to regenerate owing to its resident stem cells, also called satellite cells (SCs), that are normally quiescent. When stimulated by damage, SCs activate and expand to form new fibers. The mechanisms underlying SC proliferative progression remain poorly understood. Here we show that Dhx36, a helicase that unwinds RNA quadruplex (rG4) structures, is essential for muscle regeneration by regulating SC expansion. We find that Dhx36 (initially named RHAU) is barely expressed at quiescence and is highly induced during SC activation and proliferation. Inducible deletion ofDhx36in adult SCs causes defective proliferation and muscle regeneration after damage. System-wide mapping in proliferating SCs revealed Dhx36 binding predominantly to rG4 structures at various regions of mRNAs, while integrated polysome profiling showed that Dhx36 promotes mRNA translation via 5’UTR rG4 binding. Furthermore, we demonstrate that Dhx36 specifically regulates the translation ofGnai2mRNA by unwinding its 5’UTR rG4 structures and identify Gnai2 as a downstream effector of Dhx36 for SC expansion. Altogether our findings uncover Dhx36 as an indispensable post-transcriptional regulator of SC function and muscle regeneration through binding and unwinding rG4 structures at 5’UTR of target mRNAs.
Environmental stability of SARS-CoV-2 on different types of surfaces under indoor and seasonal climate conditions
Authors: Kwon Taeyong , Gaudreault Natasha N. , Richt Juergen A. 
Published: August 2020

AbstractWe report the stability of SARS-CoV-2 on various surfaces under indoor, summer and spring/fall conditions. The virus was more stable under the spring/fall condition with virus half-lives ranging from 17.11 to 31.82 hours, whereas under indoor and summer conditions the virus half-lives were 3.5–11.33 and 2.54–5.58 hours, respectively.
Exploring the evolutionary history of kinetic stability in the alpha-lytic protease family
Authors: Nixon Charlotte F. , Lim Shion A. , Sailer Zachary R. , Zheludev Ivan N. , Gee Christine L. , Kelch Brian A. , Harms Michael J. , Marqusee Susan 
Published: August 2020

AbstractIn addition to encoding the final tertiary fold and stability, the primary sequence of a protein encodes the folding trajectory and kinetic barriers that determines the speed of folding. How these kinetic barriers are encoded by the sequence is not well understood. Here, we use evolutionary sequence variation in the alpha-lytic protease (αLP) protein family to probe the relationship between sequence and energy landscape. αLP has an unusual energy landscape: the native state of αLP is not the most thermodynamically favored conformation and, instead, it remains folded due to a large kinetic barrier preventing unfolding. In order to fold, αLP utilizes an N-terminal pro region of similar size to the protease itself that functions as a folding catalyst. Once folded, the pro region is removed, and the native state does not unfold on a biologically relevant timescale. Without the pro region, αLP folds on the order of millennia. A phylogenetic search uncovers αLP homologs with a wide range of pro-region sizes, including some with no pro region at all. In the resulting phylogenetic tree, these homologs cluster by pro-region size. Homologs naturally lacking pro regions are thermodynamically stable, fold much faster than αLP, yet retain the same fold as αLP. Key amino acids thought to contribute to αLP’s extreme kinetic stability are lost in these homologs, further supporting their role in kinetic stability. This study highlights how the entire energy landscape plays an important role in determining the evolutionary pressures on and changes to the protein sequence.
Strategic alignment and its impact on decision effectiveness: a comprehensive model
Authors: Ghonim Mohamed A. , Khashaba Nagi M. , Al-Najaar Hameda M. , Khashan Mohamed A. 
Published: August 2020

PurposeIn recent decades, the concept of strategic alignment has been a center of concern for researchers and practitioners. This alignment is associated with the process of strategic planning to achieve high strategic performance and competitiveness. This study aims to investigate the impact of strategic alignment on decision effectiveness.Design/methodology/approachPrimary data were collected from 383 employees of the Directorate of Health Affairs in the Dakahlia Governorate in Egypt, through a self-administered questionnaire. The PLS-SEM approach was used to analyze the collected data.FindingsThe results revealed that strategic alignment significantly and positively affects decision effectiveness and its dimensions, emphasizing the importance of considering all four dimensions of strategic alignment in an integrated model to achieve the greatest impact on the decision effectiveness.Research limitations/implicationsThis study is applied to a developing country, so a comparative study between both developing and developed countries may be needed. Second, the study was restricted to the nonprofit organization, so further research may examine the profit organizations.Originality/valueDespite the existence of several studies on the relationship between strategic alignment and decision effectiveness in developed countries, studies conducted in the developing countries are still few. This is one of the earliest studies that adopt the multidimensional approach of strategic alignment in the public sector in emerging economies, which could help directors understand the interdependencies and different roles of strategic alignment dimensions in a novel comprehensive model.
Education for sustainable development in Pakistani higher education institutions: an exploratory study of students’ and teachers’ perceptions
Authors: Saqib Zulkaif Ahmed , Zhang Qingyu , Ou Jin , Saqib Khubaib Ahmad , Majeed Salman , Razzaq Amar 
Published: August 2020

PurposeThis study aims to determine the current state of implementation and effectiveness of education for sustainable development (ESD) in Pakistani higher education institutions (HEIs) using students’ and teachers’ perceptions of sustainability.Design/methodology/approachA survey on teachers and students was conducted in public and private universities in Punjab, Pakistan. Data from 1,915 students and 120 teachers were collected through semi-structured questionnaires implemented during face-to-face interviews. Students were asked to rank social, environmental and economic indicators of sustainability consciousness (SC) on a five-point Likert scale. Structural equation model and regression model are used to analyze the data. The perceptions of students and teachers were assessed from their knowledge of sustainability.FindingsThe occurrence of ESD in Pakistani HEIs is low and teachers have inadequate knowledge of sustainability. The holistic approach to ESD has a more pronounced effect on students’ SC as compared to the pluralistic approach to ESD. Also, there is a correlation between the students’ grades/class and the effectiveness of ESD. There is a need to pay more attention to implement ESD for undergraduate students in Pakistani HEIs.Originality/valueThe study stands out for using cross-sectional data from public and private universities of Pakistan. Furthermore, to the best of the authors’ knowledge, this is the first study in Pakistan which attempts to investigate the effectiveness of ESD in terms of students’ SC.
Consumer responses to pictures of co-creating consumers in marketing communications
Authors: Liljedal Karina T. , Berg Hanna 
Published: August 2020

Purpose
Co-creating consumers are often featured prominently in marketing communications for new co-created products. Previous research has only investigated the responses of non-participating consumers by describing co-creating consumers in text. This paper aims to examine consumer responses to combinations of text descriptions and pictures of co-creating consumers.

Design/methodology/approach
An experimental study used a reference group perspective to explain non-participating consumer responses to communications about co-creation with consumers in new product development.

Findings
Pictures of co-creating consumers moderate the effects of texts describing consumer co-creation on brand attitudes. The brand effects of describing the co-creating consumer in text as belonging to a dissociative group are negative when the picture looks similar to the non-participating consumers. If the co-creating consumer looks dissimilar to the in-group, the reference group text has no effect. Self–brand connection mediates these effects on brand attitudes.

Research limitations/implications
A reference group perspective is introduced as a boundary condition to the research on the communication of consumer co-creation. The effects on brand attitudes depend on the pictorial representations.

Practical implications
Companies should be advised to avoid portrayals of co-creating consumers that could cause dissociation in relevant consumer groups.

Originality/value
Neither reference group associations nor pictorial descriptions of co-creating consumers, have hitherto been investigated with regards to consumer co creation, despite the frequent inclusion of consumer imagery in advertising for consumer co-created new products.
Get-Rich Quick scheme: Malaysian current legal development
Authors: A. Rahman Aspalella , Azmi Ruzita , Mohd Yusof Rosylin 
Published: August 2020

Purpose
In Malaysia, Get-Rich-Quick scheme (GRQS) is one of the financial fraud activities prohibited under Malaysian law. The common facet of such schemes involves plans that promise unrealistic rates of returns, and this new scheme continues to proliferate every year as the list of illegal investment companies and websites are growing. Indeed, GRQS will remain proliferating as long as there are people who are easily lured by the promise that wealth can be generated with little skill, effort or time. This paper aims to explain the phenomenon of GRQS in light of the existing laws in Malaysia. This paper also highlights the current development of Australian law pertaining to GRQS for comparative purpose.

Design/methodology/approach
This paper mainly relies on statutes as its primary sources of information. As such, this paper analyses the scope and provisions of the relevant laws that regulate GRQS and compare the existing GRQS provisions that are equivalent with Australian law.

Findings
Malaysia has comprehensive laws to combat GRQS activities. However, these laws are far from perfection, and only with immediate amendments, GRQS problems can be resolved more effectively. One of the weaknesses of current Malaysian laws to tackle GRQS is the lack of more stringent punishment against the operators of GRQS as well as the participants of the scheme. A comparison with equivalent GRQS law in Australia demonstrates that Australian laws provide a wide range of punishment to the operators and prohibits participation in GRQS. More importantly, Australia regards the offense as a strict liability offense where the mens rea or guilty mind of the perpetrators is exempted. Indeed, numerous proceedings have been instituted in the Australian Court against the operators and participants of GRQS.

Originality/value
This paper analyses the scope of relevant laws in Malaysia to combat GRQS and examines the strengths and weaknesses of these laws. This paper also compares Malaysian law with equivalent GRQS-related laws available in Australia. This paper further suggests that Malaysia should regulate sterner punishment for operators and participants of the scheme and that the offense is categorized under a strict liability offense where the mens rea or guilty mind of the offender is exempted.