bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular mechanism of pyroptosis of mononuclear macrophages&nbsp;induced by pathogenic E. coli&nbsp;high pathogenicity island (HPI)&nbsp;in Yunnan Saba pigs
Authors: Shan Chunlan , Miao Shushu , Liu Chaoying , Zhao Weiwei , Zhang Bo , Yang Wei , Wang Hao , Cha Jinlong , Zhao Ru , Gao Libo , Gao Hong 
Published: February 2020

Abstract
Background In this study we evaluated the molecular mechanism by which pyroptosis is induced in mononuclear macrophages isolated from Saba pigs following infection with pathogenic E. coli high pathogenicity island (HPI). Mononuclear macrophages were divided into four treatment groups: control, Lipopolysaccharide (LPS) + adenosine triphosphate (ATP), HPI positive (+) strain and HPI negative (-) strain. The mononuclear macrophages and their culture supernatants were collected at 0.5, 3, 6, 9, 12 and 24 h after infection. DNA changes were detected by TUNEL staining and the integrity of the cell membrane was evaluated by propidium iodide (PI) staining. Changes in mRNA expression levels of NLRP3, caspase-1, IL-1β, and IL-18 gene in mononuclear macrophages were analyzed by quantitative real-time polymerase chain reaction (RT-PCR) and caspase-1 protein expression was detected by indirect immunofluorescence. IL-1β and IL-18 concentration in the mononuclear macrophage culture supernatant were measured by ELISA. Results Compared with the control group, TUNEL and PI staining of mononuclear macrophages was significantly increased following infection with the HPI + /HPI - strains ( P < 0.01 or P < 0.05), with significantly higher levels detected in the HPI + group compared with those in the HPI - group ( P < 0.01 and P < 0.05). Compared with the control group, the expression levels of NLRP3, caspase-1, IL-1β, and IL-18 in the HPI groups were upregulated after pathogenic E. coli infection, with significantly higher levels detected in the HPI + group compared with those in the HPI - group ( P < 0.01 or P < 0.05). Conclusions These findings showed that pathogenic E. coli HPI infection of Saba pigs results induced pyroptosis of mononuclear macrophages characterized by increased expression of NLRP3, caspase-1, IL-1β and IL-18 mRNA in mononuclear macrophages, the induction of cell membrane pore formation, nuclear DNA damage, and the secretion of IL-1β and IL-18 to enhance the inflammatory response.
Risk factors of hyperuricemia calculated by random forest machine learning
Authors: Gao Yuhan , Jia Shichong , Huang Chao , Meng Zhaowei , Yu Mei , Xu Tianyi , Liu Ming , Sun Jinhong , Jia Qiyu , Zhang Qing , Gao Ying , Song Kun , Wang Xing , Fan Yaguang 
Published: February 2020

Abstract
Objectives The present study aimed to develop a random forest (RF) based prediction model for hyperuricemia (HUA) and estimate associated risk factors. Methods This cross-sectional study recruited 91,690 participants (52,607 males, 39,083 females). The prediction models were derived from training sets using RF learning machine. Performances of the prediction model were evaluated in validation datasets. Significant indicators were produced after comparing between true positive set and true negative set. Odds ratio was calculated by binary logistic regression models. Results The area under the receiver-operating curve was 0.732 in males and 0.837 in females in the RF prediction models. The sensitivity, specificity and negative predictive value of the models were 0.686, 0.656 and 0.882 in males, 0.786, 0.738 and 0.978 in females, respectively. According to the feature value of each index in RF, a total of 10 explanatory variables were selected for each gender. Triglyceride, creatinine, body mass index, waist circumference, alanine transaminase, age, weight and total cholesterol were high-risk factors for HUA in both genders. Conclusion RF demonstrated good stability and strong predictive power in predicting HUA in Chinese population. People with high risk factors should be encouraged to actively control the above factors to reduce the probability of developing HUA.
Posteromedial Corner Release with the Knee in Figure-of-Four Position vs Conventional Position for Varus Knee Arthroplasty
Authors: Ji Quanbo , Zheng Qingyuan , Li Juncheng , Geng Zongjie , Ni Ming , Sun Jingyang , Zhang Guoqiang 
Published: February 2020

Abstract
Backgroud: The objective of this study is to introduce posteromedial corner release with the knee in the figure-of-four position versus the conventional position for varus knee arthroplasty. Methods : From March 2015 to September 2019, 123 patients (139 knees) with varus knee were randomly and blindly allocated to experimental group (60 patients; 68 knees) and control group (57 patients; 65 knees). Patients in experimental group underwent posteromedial corner release with the knee in the figure-of-four position; and patients in control group with the knee in the conventional position. Time for soft tissue balancing was defined as the time from the start of spacer test to the end of balance test. Length of release was defined as the distance from the osteotomy surface of the tibial plateau to the farthest structures released. The rating system of Hospital for Special Surgery (HSS) knee score was used to evaluate the clinical results. Differences were considered statistically significant at p < 0.05. Results: The mean time for soft tissue balancing was 8.4±3.3 minutes and 8.4±3.3 minutes in experimental and control group, respectively ( p <0.05). The mean length of releasing posteromedial corner structures was 35.5±13.4 mm and 27.3±9.7 mm in experimental and control group, respectively ( p <0.05). HSS scores 5 years after surgery were 95.1±16.9 and 94.8±17.2 respectively ( p >0.05). Conclusion: During varus knee arthroplasty, the posteromedial corner can be released more extensively and thoroughly when the knee is placed in the figure-of-four position.
Acute popliteal artery thrombogenesis after total knee arthroplasty: a case report
Authors: Liu Ri , Gao Lishu , Wei Haiqiang , Xie Kunnan 
Published: February 2020

Abstract
Total knee arthroplasty (TKA) is a type of artificial joint replacement surgery. An acute lower limb arterial embolism after TKA is extremely rare. Although the incidence of acute lower extremity popliteal artery thrombogenesis is very low, orthopedists need to be alert to the complication because it may lead to amputation and other serious consequences. We report a 65-year-old man who had 38 years of post-traumatic pain in the left knee joint, which has worsened in the past 3 years, thus limiting mobility. He had an acute popliteal artery thrombogenesis after undergoing a TKA. Although orthopedic and vascular surgeons took active measures in a timely fashion, the patient still had lower extremity dysfunction. After a TKA, patients should be actively instructed to perform rehabilitation exercises to prevent thromboses.
Clinical analysis of retinal detachment caused by ocular siderosis
Authors: Feng Chao , Xu Chong , Kang Xueqin , Wu Jianhua 
Published: February 2020

Abstract
Background: Until now, there was no report about retinal detachment caused by ocular siderosis. The aim of this study was to analyse the clinical characteristics of retinal detachment caused by ocular siderosis. Methods: A retrospective cohort analysis was performed to analyse the clinical characteristics of retinal detachment caused by ocular siderosis in Aier Eye Hospital of Wuhan University from January 2016 to December 2019. Results: There were 12 patients (12 eyes) with retinal detachment caused by ocular sclerosis, 11 males (11 eyes) and 1 female (1 eye), aged 20-54 years. The median best corrected visual acuity (LogMAR) before and after surgery was 2.6 and 1.4, respectively. Retinal detachment recurred after the removal of silicone oil in four patients, all of whom had retinal tears. Conclusion: The prognosis of retinal detachment caused by ocular siderosis is poor, and retinal detachment easily recurs when retinal tears exist.
Consistency evaluation of intraocular lens power calculation methods in eyes that have undergone small-incision lenticule extraction
Authors: Li Jiawen , Chen Bo , Zhou Youhui , Xie Jing , Huo Shujia 
Published: February 2020

Abstract
Background To compare the differences in the consistency of intraocular lens (IOL) power calculations with four different formulas for cataract surgery after small-incision lenticule extraction (SMILE), and to select the most suitable formula to calculate the IOL power of cataract patients after SMILE. Methods 98 eyes of 51 patients who underwent small-incision lenticule extraction (preoperative spherical equivalents, -2.05 to -10.10D) between June 2019 to July 2019 in our department were included, all surgeries were performed by the same surgeon. IOLMaster 500 was used for biometric measurement and Haigis-L correction formula was calculated. AL-Scan was used to calculate Shammas-PL correction formula, Camellin-Calossi formula and Barret True K formula. We simulated the intraocular lens calculation before cataract surgery in all eyes with these four formulas. The consistency of the calculated IOL powers were evaluated by correlation analysis, one-way ANOVA, and Bland-Altman analysis.Results The average values of Barret True K formula, Shammas-PL correction formula, Camellin-Calossi formula and Haigis-L correction formula are 22.32±1.14 D, 23.28±1.21 D, 22.91±1.26 D, 23.54±1.23 D respectively. Simple correlation analysis: The average values of these four formulas were correlated, and the correlation coefficients were 0.94(Barrett Ture K and Shammas-PL), 0.98 (Barrett Ture K and Camellin-Calossi), 0.89(Barrett Ture K and Haigis-L), 0.98(Haigis-L and Shammas-PL), 0.88(Haigis-L and Camellin-Calossi) and 0.93(Shammas-PL and Camellin-Calossi)(significant, p=0.00); One-way ANOVA: There was no significant difference between the Shammas-PL correction formula and the Haigis-L correction formula. The Camellin-Calossi formula and the Barret True K formula were significantly different from the Haigis-L correction formula. Bland-Altman analysis: for the difference-mean scatter plot and the ratio-mean scatter plot: the maximum allowable error of the 95% limit is 9.61%, 7.45%, and 2.99%, respectively, indicating that the results are consistent. Conclusions Haigis-L and Shammas-PL formulas had good consistency when calculating the IOL power in eyes after SMILE.
Evaluation of pemetrexed in the treatment of epithelial ovarian cancer, fallopian tube cancer, and primary peritoneal cancer: a retrospective study
Authors: Wu Yanmei , Jiang Jie 
Published: February 2020

Abstract
Background: The aim of this study was to evaluate the efficacy and safety of pemetrexed in the treatment of epithelial ovarian cancer, fallopian tube cancer, and primary peritoneal cancer.Results: Patients with platinum-sensitive and platinum-resistant epithelial ovarian cancer who received pemetrexed disodium chemotherapy at Qilu Hospital of Shandong University between January 2015 and December 2018 were identified. Dose delay and adjustment were permitted when serious side effects occurred. To observe the efficacy rate and follow up on its side effects, progression-free survival and overall survival after chemotherapy were monitored. A total of 96 cases were collected, and 340 cycles of chemotherapy were administered. The overall response rate (ORR) was 32.29% (31 patients), where 16 patients (16.67%) had a complete response, and 15 patients (15.62%) had a partial response. There were 68 platinum-resistant patients and 28 platinum-sensitive patients. The ORRs of platinum-sensitive and platinum-resistant patients were 42.86% (12 patients) and 27.94% (19 patients), respectively, p = 0.16. The median progression-free survival (PFS) was 3.52 months and 2.97 months (HR0.62, 95% CI, 0.37-1.02), respectively, and there was no significant difference (P = 0.06). The median overall survival (OS) of the two groups was 36 months and 18 months, respectively, and the difference was statistically significant (p= 0.01). There was no drug-related death in all patients, and all of the side effects were mild. The most common side effects were myelosuppression, gastrointestinal reactions and hepatotoxicity.Conclusion: Pemetrexed disodium is effective not only for platinum-sensitive but also platinum-resistant epithelial ovarian cancer. And the side effects are tolerable. Pemetrexed disodium may be a choice for epithelial ovarian cancer, fallopian tube cancer, and primary peritoneal cancer.
Experiences of the mothers who have tried to intervene with their children’s obesity: A qualitative study
Authors: Piao Meihua , Kim Jeongeun , Byun Ahjung , Joung Woo Joung 
Published: February 2020

Abstract
Background: Childhood obesity has become increasingly prevalent not only worldwide but also in South Korea. It is known that the lifestyle habits and parents’ education were found to be effective. The aim of this research is to explore the experiences of obese children and their mothers regarding obesity and identifying barriers and facilitators to optimize their weight control practices.Methods: A qualitative method of focus group interview was used for the data collection. Overweight or obese children aged between 12 and 14 and their mothers participated in the interview.Results: Five pairs of participants (a mother and a child) were interviewed. Mothers tended to be aware of their children’s weight issue and tried to manage it themselves by supporting them more actively. They made considerable efforts to change their children’s lifestyles with regard to diet and exercise. Additionally, children expected to let their mothers guide them in order to control body weight. However, barriers to achieving their desired body weight were associated with their social contexts that prevented them from continuing their healthy lifestyles. Social context was influential for addressing childhood obesity. The busy and competitive environments in which they live forced the children into busy lifestyles that hindered them from performing their healthy habit promptly.Conclusions: The results suggest that interventions should be focused on setting a realistic goal and engaging in simple behaviors that could be performed in daily routine. Moreover, ensuring access to a communication channel with other parents or healthcare providers is important.
Transcriptome and small RNA combined sequencing analysis of cold tolerance response in non-heading Chinese cabbage
Authors: Wang Jin , Zhang Qinxue , You Xiong , Hou Xilin 
Published: February 2020

Abstract
Background: Non-heading Chinese cabbage ( Brassica rapa ssp. chinensis ), as an important leaf vegetable grown worldwide. However, there is currently no enough transcriptome and small RNA combined sequencing analysis of cold tolerance, which hinders further functional genomics research.Results: In this study, 63.43 Gb of clean data was obtained from the transcriptome analysis. The clean data of each sample reached 6.99 Gb, and the basic percentage of Q30 was 93.68% and above. The clean reads of each sample were sequence aligned with the designated reference genome ( Brassica rapa, IVFCAASv1 ), and the efficiency of the alignment varied from 81.54% to 87.24%. According to the comparison results, 1,860 new genes were discovered, of which 1,613 were functionally annotated. Among them, 13 common differentially expressed genes were detected in all materials, including 7 up-regulated and 6 down-regulated. At the same time, we used quantitative real-time PCR to confirm the changes of these gene expression levels. In addition, we sequenced miRNA of the same material. Our findings revealed a total of 34,182,333 small RNA reads, 88,604,604 kinds of small RNA, among which the most common size was 24 nt. In all materials, the number of common differential miRNAs is 8. According to the corresponding relationship between miRNA and its target genes, we carried out Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis on the set of target genes that each group of differentially expressed miRNAs. Through the analysis, it is found that the distribution of candidate target genes in different materials is different. We not only use transcriptome sequencing and small RNA sequencing, but also use experiment to prove that the expression level of differentially expressed genes are obtained by sequencing. Sequencing combined with experiments proved the mechanism of some differential gene expression levels after low temperature treatment.Conclusions: In all, this study provides a resource for genetic and genomic research under abiotic stress in Pak-choi.
Predictors of atrial fibrillation in patients with type 2 diabetes mellitus: a propensity score-matched case-control study.
Authors: Li Zexiong , Luo Yanting , Huang Zhuoshan , Yuan Xiaosi , Peng Long , Xie Xujing , Zhu Jieming , Liu Jinlai 
Published: February 2020

Abstract
Background Compared to people without diabetes, people with type 2 diabetes mellitus (T2DM) have an increased risk of developing atrial fibrillation (AF). Possible predictors of AF have not been adequately investigated in T2DM. We aimed to identify the factors associated with AF in such patients. Methods A total of 2,682 patients with T2DM, who was admitted to Department of Cardiovascular Medicine, the Third Affiliated Hospital, Sun Yat-sen University between 1 June, 2015 to 1 Nov, 2019, were enrolled in the study. 641 patients were excluded. There were 122 patients with AF and 1,919 patients without AF. 1:1 propensity scores match was performed according to age and gender. Patients were divided into the two groups: with AF (122patients) and without AF (122patients). Clinical, demographic, echocardiographic, laboratorial data and medicine were compared between the groups. Results There was significant statistical difference in neutrophil, monocyte, creatinine, total cholesterol (TC), triglyceride, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (ApoA1), apolipoprotein B100 (ApoB100), monocyte to high-density lipoprotein ratio (MHR), fasting glucose (FG), left atrial diameter (LAD), ejection fraction(EF), antiplatelet therapy and the prevalence of heart failure between the two groups. On multivariate logistic regression analysis, the increase of MHR, FG and LAD, as well as the decrease of TC and antiplatelet therapy were independent predictors of AF. On receiver operating characteristic (ROC) curve analysis, using a cut-off level of 0.568, MHR predicted AF with a sensitivity of 65.6%, a specificity of 76.2%, and an AUC of 0.736(95% CI 0.673-0.800). Using a cut-off level of 0.507, the combination of TC, MHR, FG, LAD and antiplatele therapy predicted AF with a sensitivity of 73.0%, a specificity of 84.4%, and an AUC of 0.862(95% CI 0.816-0.907). Conclusions The increase of MHR, FG and LAD, as well as the decrease of TC and antiplatelet therapy were independent predictors of AF. MHR only, as well as the combination of TC, MHR, FG, LAD and antiplatelet therapy can better predict the risk of AF in patients with T2DM.
Long-term survival of cemented versus uncemented total hip arthroplasty in the treatment of acute femoral neck fracture: A retrospective observational study with a mean 10-year follow-up
Authors: Han Xiulan , Yu Weiguang , Lin Jinluan , Zhao Mingdong , Han Guowei , Zhang Chao Xin 
Published: February 2020

Abstract
Background Despite the increased use of uncemented total hip arthroplasty (UTHA), there is little evidence of its superiority over cemented total hip arthroplasty (CTHA). The purpose of this retrospective study was to compare the long-term survivorship and Harris Hip Scores (HHSs) of CTHA versus UTHA in the treatment of acute femoral neck fractures (FNFs). Methods Data involving 224 hips (CTHA, n= 112; UTHA, n=112) that underwent primary surgery in our medical institutions during 2005-2017 were analysed retrospectively. The primary endpoint was the risk of all-cause revision. The difference in the risk of all-cause revision between groups was assessed by Kaplan-Meier survival analysis with a log-rank test and Cox regression analysis. Results The mean follow-up from surgery was 10 years (range, 3 - 13 years). Kaplan-Meier estimated that the 10-year implant survival was 98.1% (CI: 96.1–98.5) in the CTHA group and 96.2% (CI: 95.2–97.3) in the UTHA group (p = 0.030). The adjusted Cox regression analysis demonstrated a lower risk of revision in CTHA than in UTHA (hazard ratio [HR] = 1.4, 95% confidence interval [CI] = 1.1-2.6, p = 0.000). At the final follow-up, significant differences were detected in HHS (85.10[±12.21] for CTHA vs. 79.11[±13.19] for UTHA). Conclusion This retrospective analysis demonstrates that CTHA has superior survival to UTHA, with a significantly reduced revision risk and higher functional outcome scores. Further follow-up is necessary to verify whether the CTHA advantage persists over time.
Exploring the diagnostic value of multilayer spiral computed tomography (MSCT) in thoracogastric airway fistula
Authors: Zhao Yue , Zeng Yan-Wei , Yin Mei-Pan , Li Zhen , Li Chun-Xia , Ma Yao-Zhen , Xie Peng-Fei , Wu Gang 
Published: February 2020

Abstract
Background Thoracostomach-airway fistula is a rare complication of esophageal-cancer resection. We intended to explore the diagnostic value of multilayer spiral computed tomography (MSCT) in thoracostomach-airway fistula, to enhance its clinical understanding; evaluate an effective, convenient, and non-invasive diagnostic method; and provide the fundamentals for subsequent treatment. Methods The MSCT images and complete clinical data of 216 consecutive patients diagnosed with thoracogastric airway fistula were retrospectively analyzed. Results MSCT was able to determine the exact location, size of the fistula, and the adjacent bronchus between the fistula and bronchus in 192 out of 216 patients with thoracogastric airway fistula. All patients had pulmonary lesions including emphysema (n=68), ground glass density (n=25), bronchial wall thickening (n=28), patchy high densities along the lung texture (n=207), pulmonary consolidation (n=104), atelectasis (n=11), pulmonary nodules (n=53), bronchiectasis (n=19), lobular septal thickening (n=5), cavity (n=14), and pulmonary interstitial fibrosis (n=6). Further, 95 cases showed pleural effusion, 184 showed pleural thickening, 4 had pneumothorax, and 2 had subcutaneous pneumothorax. 12 patients with thoracogastric airway fistula had anastomotic wall thickening (mean: 20.63±9.57, range: 9.19–43.37 mm), while 13 patients showed thoracic gastric wall thickening (mean: 22.83(±)5.58 mm, range: 12.32–31.24 mm) on. 23 patients showed distant metastasis on MSCT. Conclusions MSCT can accurately display the location and size of thoracogastric airway fistula, as well as lung lesions, tumor recurrence, and distant metastasis, and provide the basis for the next therapy.