bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nephrectomy Does not Exacerbate Cancellous Bone loss in Thalassemic Mice
Authors: Lotinun Sutada , Atjanasuppat Korakot , Limsuvech Jutatip , Leelahavanichkul Asada , Svasti Saovaros , Krishnamra Nateetip 
Published: May 2020

AbstractPatients with β-thalassemia have an increased risk of developing chronic kidney disease which is associated with osteoporosis and periodontitis. The purpose of this study was to evaluate mandibular and femoral bone change in heterozygous β-globin knockout (BKO) mice following 5/6 nephrectomy (Nx). Female and male BKO mouse blood smears demonstrated microcytic hypochromic anemia. Serum urea nitrogen, creatinine, calcium, and phosphorus levels were not changed in BKO mice. Nx increased the serum levels of urea nitrogen in both wild type (WT) and BKO mice and the level was much higher in BKO males. Serum level of creatinine was increased in Nx WT but not BKO mice. However, serum calcium and phosphorus levels were not altered. Nx induced comparable renal fibrosis in BKO mice and WT controls. Bone loss was observed in mandibular cancellous bone but not cortical bone of both male and female BKO mice. Nx decreased cancellous bone volume and cortical thickness in WT. Interestingly, BKO mice were resistant to Nx-induced cancellous bone loss. However, cortical thickness and cortical bone mineral density were reduced in Nx male BKO mice. Nx increased mRNA levels of type I collagen, Osx and Trap in WT but not BKO mice. Similarly, Nx reduced cancellous bone volume in femurs and increased osteoblast number and osteoclast number in WT not BKO mice. Serum FGF23 and erythropoietin levels were markedly increased in BKO mice. Nx decreased serum erythropoietin but not FGF23 levels. Since WT treated with erythropoietin exhibited a significant reduction in cancellous bone volume, it was possible that lower level of erythropoietin in Nx BKO mice prevented the Nx-induced cancellous bone loss.
Cell-specific metabolomic responses to injury: novel insights into blood-brain barrier modulation
Authors: Huang Sheng-Fu , Fischer Sabrina , Koshkin Alexey , Laczko Endre , Fischer David , Ogunshola Omolara O. 
Published: May 2020

AbstractOn one hand blood-brain barrier (BBB) disturbance aggravates disease progression, on the other it prevents drug access and impedes therapeutic efficacy. Effective ways to modulate barrier function and resolve these issues are sorely needed. Convinced that better understanding of cell-oriented BBB responses could provide valuable insight, and the fact that metabolic dysregulation is prominent in many vascular-related pathological processes associated with BBB disturbance, we hypothesized that differential cell-specific metabolic adaptation majorly influences physiological and pathological barrier functionality. Untargeted liquid chromatography–mass spectrometry (LC-MS) metabolomic profiling was used to obtain individual biochemical fingerprints of primary astrocytes (AC) and brain endothelial cells (EC) during normoxic conditions and increasing hypoxic/ischemic injury and thus a functional readout of cell status. Bioinformatic analyses showed each cell had a distinct metabolic signature. Corroborating their roles in BBB and CNS protection, AC showed an innate ability to dynamically alter their metabolome depending on the insult. Surprisingly, in complete contrast, EC largely maintained their normoxic characteristics in injury situations and their profiles diverged from those of non-brain origin. Tissue specificity/origin is clearly important when considering EC responses. Focusing on energy capacity and utilization we discuss how cell-specific metabolic adaptive capabilities could influence vascular stability and the possibility that altering metabolite levels may be an effective way to modulate brain EC function. Overall this work novel insight into cell-associated metabolic changes, and provides a powerful resource for understanding BBB changes during different injury scenarios.
WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat
Authors: Paudel Bimal , Zhuang Yongbin , Galla Aravind , Dahal Subha , Qiu Yinjie , Ma Anjun , Raihan Tajbir , Yen Yang 
Published: May 2020

AbstractFusarium head blight (FHB) is a severe disease of wheat (Triticum aestivum L.). Qfhb1 is the most important quantitative trait locus (QTL) for FHB resistance. We previously identified wheat gene WFhb1-1 (aka WFhb1-c1) as a candidate for FHB resistance gene. Here we report that WFhb1-1 has been cloned. The gene (GenBank # KU304333.1) consists of a single exon, encoding a putative membrane protein of 127 amino acids. WFhb1-1 protein produced in Pichia pastoris inhibits growth of both F. graminearum and P. pastoris in culture. Western Blotting with anti- WFhb1-1 antibody revealed a significant decrease (p < 0.01) in WFhb1-1 accumulation, 12 hours post Fusarium inoculation in non-Qfhb1-carrier wheat but not in Qfhb1-carrier wheat. Overexpressing WFhb1-1 in non-Qfhb1-carrier wheat led to a significant decrease (p < 0.01) in Fusarium-damaged rachis rate, Fusarium-diseased kernel rate and DON content in harvested kernels, while silencing WFhb1-1 in Qfhb1-carrier wheat resulted in a significant increase (p < 0.01) in FHB severity. Therefore, WFhb1-1 is an important FHB resistance gene with a potential antifungal function and probably a key functional component of Qfhb1 in wheat. A model regarding how WFhb1-1 functions in FHB resistance/susceptibility is hypothesized and discussed.
Experimental and numerical study on photocatalytic activity of the ZnO nanorods/CuO composite film
Authors: Nguyen Dung T. , Tran Minh Duc , Van Hoang Thanh , Trinh Duc Thien , Pham Duc Thang , Nguyen Dinh Lam 
Published: May 2020

AbstractThe photocatalytic activity of the ZnO NRs/CuO composite film was investigated by using both experimental and numerical methods. The ZnO NRs/CuO composite film exhibits significantly enlarge absorption range to visible-light and suppress the recombination rate of the photogenerated electron-hole pairs, which can be well utilized as a photocatalyst. The ZnO NRs/CuO composite film also presents good stability, and reusability, and durability for photo-decomposition purpose. The optimal ZnO NRs/CuO composite film contains 1μ-thick of CuO film and 10 nm-thick of ZnO NRs film. The donor concentration in ZnO NRs film should be lower than 1016 cm−3. The short circuit current density of the optimal composite film is 25.8 mA/cm2 resulting in the calculated pseudo-order rate constant of 1.85 s−1. The enhancement in degradation efficiency of this composite film is attributed to the inner electric field and large effective surface area of ZnO NRs film.
Is step width decoupled from pelvic motion in human evolution?
Authors: Kikel Michelle , Gecelter Rachel , Thompson Nathan E. 
Published: May 2020

AbstractHumans are the only primate that walk bipedally with adducted hips, valgus knees, and swing-side pelvic drop. These characteristic frontal-plane aspects of bipedalism likely play a role in balance and energy minimization during walking. Understanding when and why these aspects of bipedalism evolved also requires an understanding of how each of these features are interrelated during walking. Here we investigated the relationship between step width, hip adduction, and pelvic list during bipedalism by altering step widths and pelvic motions in humans in ways that both mimic chimpanzee gait as well as an exaggerated human gait. Our results show that altering either step width or pelvic list to mimic those of chimpanzees affects hip adduction, but neither of these gait parameters dramatically affects the other in ways that lead to a chimpanzee-like gait. These results suggest that the evolution of valgus knees and narrow steps in humans may be decoupled from the evolution of the human-like pattern of pelvic list. While the origin of narrow steps in hominins may be linked to minimizing energetic cost of locomotion, the origin of the human-like pattern of pelvic list remains unresolved.
Paediatric computed tomography and subsequent risk of leukaemia, intracranial malignancy and lymphoma: a nationwide population-based cohort study
Authors: Li I-Gung , Yang Yao-Hsu , Li Yiu-Tai , Tsai Yuan-Hsiung 
Published: May 2020

AbstractRed bone marrow and brain tissue are highly radiosensitive in children. We investigate the relationship between childhood computed tomography (CT) exposure and leukaemia, intracranial malignancy and lymphoma. All participants in the study were aged less than 16 years. A total of 1,479 patients in the leukaemia group, 976 patients in the intracranial malignancy group and 301 patients in the lymphoma group were extracted from the Catastrophic Illness Certificate Database in Taiwan as the disease group. In total, 126,677 subjects were extracted from the Longitudinal Health Insurance Database 2010 of the Taiwan National Health Insurance Research Database as the non-disease group. The odds ratios (ORs) and 95% confidence intervals (CIs) for childhood CT exposure and times of childhood CT were estimated. Childhood CT exposure was correlated to the intracranial malignancy group in both one-year (OR = 1.95, 95% CI 1.40–2.71, p < 0.001) and two-year (OR = 1.56, 95% CI 1.04–2.33, p = 0.031) exclusion periods. The time of childhood CT was also correlated to intracranial malignancy in both one-year (OR = 1.69, 95% CI 1.34–2.13, p < 0.001) and two-year (OR = 1.55, 95% CI 1.17–2.04, p = 0.002) exclusion periods. The results indicated that childhood CT exposure was correlated with an increased risk of future intracranial malignancy.
Precometary organic matter: A hidden reservoir of water inside the snow line
Authors: Nakano Hideyuki , Hirakawa Naoki , Matsubara Yasuhiro , Yamashita Shigeru , Okuchi Takuo , Asahina Kenta , Tanaka Ryo , Suzuki Noriyuki , Naraoka Hiroshi , Takano Yoshinori , Tachibana Shogo , Hama Tetsuya , Oba Yasuhiro , Kimura Yuki , Watanabe Naoki , Kouchi Akira 
Published: May 2020

AbstractThe origin and evolution of solar system bodies, including water on the Earth, have been discussed based on the assumption that the relevant ingredients were simply silicates and ices. However, large amounts of organic matter have been found in cometary and interplanetary dust, which are recognized as remnants of interstellar/precometary grains. Precometary organic matter may therefore be a potential source of water; however, to date, there have been no experimental investigations into this possibility. Here, we experimentally demonstrate that abundant water and oil are formed via the heating of a precometary-organic-matter analog under conditions appropriate for the parent bodies of meteorites inside the snow line. This implies that H2O ice is not required as the sole source of water on planetary bodies inside the snow line. Further, we can explain the change in the oxidation state of the Earth from an initially reduced state to a final oxidized state. Our study also suggests that petroleum was present in the asteroids and is present in icy satellites and dwarf planets.
Primary proprioceptive neurons from human induced pluripotent stem cells: a cell model for afferent ataxias
Authors: Dionisi Chiara , Rai Myriam , Chazalon Marine , Schiffmann Serge N. , Pandolfo Massimo 
Published: May 2020

AbstractHuman induced pluripotent stem cells (iPSCs) are used to generate models of human diseases that recapitulate the pathogenic process as it occurs in affected cells. Many differentiated cell types can currently be obtained from iPSCs, but no validated protocol is yet available to specifically generate primary proprioceptive neurons. Proprioceptors are affected in a number of genetic and acquired diseases, including Friedreich ataxia (FRDA). To develop a cell model that can be applied to conditions primarily affecting proprioceptors, we set up a protocol to differentiate iPSCs into primary proprioceptive neurons. We modified the dual-SMAD inhibition/WNT activation protocol, previously used to generate nociceptor-enriched cultures of primary sensory neurons from iPSCs, to favor instead the generation of proprioceptors. We succeeded in substantially enriching iPSC-derived primary sensory neuron cultures for proprioceptors, up to 50% of finally differentiated neurons, largely exceeding the proportion of 7.5% normally represented by these cells in dorsal root ganglia. We also showed that almost pure populations of proprioceptors can be purified from these cultures by fluorescence-activated cell sorting. Finally, we demonstrated that the protocol can be used to generate proprioceptors from iPSCs from FRDA patients, providing a cell model for this genetic sensory neuronopathy.
Enhanced 4Pi single-molecule localization microscopy with coherent pupil based localization
Authors: Liu Sheng , Huang Fang 
Published: May 2020

AbstractOver the last decades, super-resolution techniques have revolutionized the field of fluorescence microscopy. Among them, interferometric or 4Pi microscopy methods exhibit supreme resolving power in the axial dimension. Combined with single-molecule detection/localization and adaptive optics, current 4Pi microscopy methods enabled 10–15 nm isotropic 3D resolution throughout whole cells. However, further improving the achieved 3D resolution poses challenges arising from the complexity of single-molecule emission patterns generated by these coherent single-molecule imaging systems. These complex emission patterns render a large portion of information carrying photons unusable. Here, we introduce a localization algorithm that achieves the theoretical precision limit for a 4Pi based single-molecule switching nanoscopy (4Pi-SMSN) system, and demonstrate improvements in localization precision, accuracy as well as stability comparing with state-of-the-art 4Pi-SMSN methods.
Timekeeping in the hindbrain: a multi-oscillatory circadian centre in the mouse dorsal vagal complex
Authors: Chrobok Lukasz , Northeast Rebecca C. , Myung Jihwan , Cunningham Peter S. , Petit Cheryl , Piggins Hugh D. 
Published: May 2020

AbstractMetabolic and cardiovascular processes controlled by the hindbrain exhibit 24 h rhythms, but the extent to which the hindbrain possesses endogenous circadian timekeeping is unresolved. Here we provide compelling evidence that genetic, neuronal, and vascular activities of the brainstem’s dorsal vagal complex are subject to intrinsic circadian control with a crucial role for the connection between its components in regulating their rhythmic properties. Robust 24 h variation in clock gene expression in vivo and neuronal firing ex vivo were observed in the area postrema (AP) and nucleus of the solitary tract (NTS), together with enhanced nocturnal responsiveness to metabolic cues. Unexpectedly, we also find functional and molecular evidence for increased penetration of blood borne molecules into the NTS at night. Our findings reveal that the hindbrain houses a local network complex of neuronal and non-neuronal autonomous circadian oscillators, with clear implications for understanding local temporal control of physiology in the brainstem.
HCV Seroreactivity and Detection of HCV RNA in Hepatitis Cases
Authors: Nagamoti Mahantesh B , Patil Chidanand S , Jyoti M Nagamoti 
Published: May 2020

Background:
Hepatitis C virus (HCV) known to be associated with wide variety of liver pathology. It is less studied in India as compared to western region.

Methods:
Suspected patients sera screened for HCV by ELISA and confirmed with reverse transcription polymerase chain reaction (RT-PCR) along with routine investigations and liver profile. All HCV positive patients were undergone liver biopsy.

Results:
All 24 HCV ELISA reactive and two ELISA indeterminate sera are confirmed by RT- PCR. The liver biopsy of these patients showed normal picture (19.2%), Acute hepatitis (11.5%), Chronic hepatitis (23.7%), Cirrhosis (34.72%), Hepato-cellular carcinoma (HCC) (15.38%). ALT levels were not significant.

Conclusion:
All the suspected HCV cases need to be confirmed for HCV by RT-PCR.
Shoulder Periarthritis and its Imaging Features in Patients with Type 1 Diabetes Mellitus
Authors: Nair Abhilash , Kandasamy Devasenathipathy , Sharma Raju , Nag HL , Singh Upiderpal , Bhatia Himani , Saha Soma , Tandon Nikhil , Goswami R. 
Published: May 2020

Aim:
There is limited information on periarthritis/adhesive capsulitis of the shoulder (ACS) in patients with type-1-diabetes mellitus (T1D). We assessed the prevalence and characteristics of ACS in patients with type-1-diabetes mellitus.

Methods:
Consecutive 267 patients attending 'Diabetes of Young Clinic' were screened for ACS. Those with clinical features of ACS were further assessed by 'shoulder pain and disability index' (SPADI), radiograph and MRI of the shoulder. The average glycemic status (HbA1c) during preceding 2 years was assessed in patients with and without ACS. Controls were age and sex matched healthy subjects (1:1 ratio).

Results:
Sixteen of 267 patients (6.0%) with type-1-diabetes had clinical features of ACS, unlike none of the healthy controls (P < 0.001). Internal and external rotation of the shoulders was the most frequently restricted movements in ACS. Thickened coracohumeral ligament and axillary pouch obliteration was characteristic MRI feature, present in 80.0% in 73.3% cases, respectively. Though 14/16 type-1-diabetes patients with ACS were symptomatic, they never reported these complaints in diabetic clinic with the treating physicians. On regression analysis (odds ratio; 95% CI), duration of diabetes (1.1; 1.03-1.17, P < 0.01), retinopathy (3.6; 1.05-12.52, P = 0.04), and limited joint mobility (6.4; 1.88-21.95, P < 0.01) were independent predictors for presence of ACS in type-1-diabetes. The mean HbA1c and lipid levels were comparable in patients with or without ACS.

Conclusions:
Six percent of patients with type-1-diabetes had ACS, which can be detected on clinical screening and confirmed by imaging to help initiate early treatment.
Time Trends in Prevalence of Anaemia in Adolescent Girls
Authors: Kalaivani K , Ramachandran Prema 
Published: May 2020

Introduction:
Anaemia in adolescent girls has been recognised as a major public health problem. The Mid-day meal programme guidelines envisage inclusion of 75 g/day of vegetables and use of iron fortified iodised salt for hot cooked meal. The National Iron Plus Initiative envisages weekly iron-folic acid (IFA) supplementation for adolescent girls; however, coverage and compliance have been reported to be low. Data from national surveys carried out in the last two decades were analysed to assess changes, if any, in Hb levels and prevalence of anaemia in adolescent girls.

Material and Methods:
Raw data from National Family Health Surveys (NFHS) -2, -3, and -4, District Level Household Surveys (DLHS) 2 and 4, and Annual Health Survey-related to Clinical, Anthropometric and Biochemical Components (AHS-CAB) were analysed to assess mean Hb, prevalence of anaemia and frequency distribution of Hb in adolescent girls. Comparison in these parameters was made between non-pregnant girls 10-14 years and 15-19 years of age in DLHS-2, -4 and AHS-CAB; in the 15-19 year age group comparisons were made between pregnant and non-pregnant girls in NFHS series and DLHS AHS series.

Results:
There were no clear and consistent changes in mean Hb, prevalence of anaemia and frequency distribution of Hb in pregnant and non-pregnant adolescent girls between NFHS-2, -3 and -4 either at national or at State level. However, there was a 0.7 and 1.3 g/dL increase in mean Hb levels in non-pregnant girls (10-19 yrs) between DLHS-2 and AHS-CAB and DLHS-4 States, respectively. The increase in mean Hb of pregnant girls (15-19 yrs) was 1.1 g/dL and 1.4g/dL in AHS-CAB and DLHS 4 States, respectively. There was significant reduction in prevalence of anaemia in both pregnant and non-pregnant girls between DLHS 2 and DLHS 4 and AHS-CAB at the aggregate level for each survey and in all States except Uttarakhand.

Conclusion:
There has been some improvement in Hb levels in adolescent girls in the last two decades. Improving dietary intake of vegetables and promoting use of iron fortified iodised salt in all households in the country have to be taken up so that iron intake across all age groups improves. This when combined with daily IFA supplementation for three months in a year in adolescent girls, might lead to sustained improvement in Hb.
Room-temperature autonomous self-healing glassy polymers with hyperbranched structure
Authors: Wang Hao , Liu Hanchao , Cao Zhenxing , Li Weihang , Huang Xin , Zhu Yong , Ling Fangwei , Xu Hu , Wu Qi , Peng Yan , Yang Bin , Zhang Rui , Kessler Olaf , Huang Guangsu , Wu Jinrong 
Published: May 2020

Significance
Glassy polymers are widely used as structural materials in aerospace vehicles, airplanes, automobiles, biomedical devices, and so on. However, glassy polymers are difficult to self-heal at room temperature due to their frozen molecules. For example, the window glass of airplanes made of polycarbonate cannot repair mechanical damages autonomously, which could lead to a catastrophe. To impart glassy polymers with self-healing ability, we take advantage of the high mobility of hyperbranched polymers, and introduce hydrogen complementary moieties onto the hyperbranched structure, enabling the construction of a series of autonomous room-temperature self-healing glassy polymers.
Definiteness in Wenzhounese Chinese in the Netherlands and in China
Authors: Aalberse Suzanne , Andringa Sible , Faber Martina , Lippe Phine 
Published: May 2020

Abstract
By comparing two generations of speakers in China and the Netherlands, we investigated whether Wenzhounese Chinese as spoken by heritage speakers in the Netherlands might be subject to change due to its contact with the Dutch language. To this end, we considered how nouns referring to already mentioned referents were encoded, hypothesizing an increase in overtly marked already mentioned referents in the speech of second generation speakers in the Netherlands. Also, shifts in the use of classifier and demonstrative constructions were investigated as carriers of definite meanings. The data showed clear generation effects, especially in the use of classifier and demonstrative constructions, but no location effects. Although the interpretation of the data is compromised by the fact that Wenzhounese as spoken China is under threat, the study suggests that innovations found in heritage speakers may not necessarily be related to the heritage scenario, but can also reflect more general contact effects or internally motivated changes.
The Frequency Code and gendered attrition and acquisition in the German-English heritage language community in Vancouver, Canada
Authors: de Leeuw Esther 
Published: May 2020

Abstract
The paper investigates pitch level and span in a group of German L1-English L2 late bilinguals in comparison to two monolingual control groups. The late bilinguals had moved to Vancouver, Canada in adulthood, and had been living in Vancouver for an average of 40 years. The results indicate that the bilingual males increased their pitch in both English and German, and widened their pitch span, therefore indexing non-aggressive, friendly behaviour, but deviating from both monolingual pitch norms. Thus, the results offer evidence that pitch changes are at least in part dependent on the social and political environment in which they are embedded, as a low pitch level is associated with dominance and aggression which would boost the negative image of the Vancouver German community due to their ethnic origin after WWII.