bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive genetic diagnosis of Japanese patients with severe proteinuria
Authors: Nagano China , Yamamura Tomohiko , Horinouchi Tomoko , Aoto Yuya , Ishiko Shinya , Sakakibara Nana , Shima Yuko , Nakanishi Koichi , Nagase Hiroaki , Iijima Kazumoto , Nozu Kandai 
Published: January 2020

AbstractNumerous disease-causing gene mutations have been identified in proteinuric diseases, such as nephrotic syndrome and glomerulosclerosis. This report describes the results of comprehensive genetic diagnosis of Japanese patients with severe proteinuria. In addition, the report describes the clinical characteristics of patients with monogenic disease-causing mutations. We conducted comprehensive gene screening of patients who had either congenital nephrotic syndrome, infantile nephrotic syndrome, steroid-resistant nephrotic syndrome, or focal segmental glomerular sclerosis. Using targeted next-generation sequencing, 60 podocyte-related genes were screened in 230 unrelated patients with proteinuria. A retrospective review of clinical data was conducted for these patients. We detected monogenic disease-causing mutations in 30% (69 of 230) of patients among 19 of the screened genes. Common genes with disease-causing mutations were WT1 (25%), NPHS1 (12%), INF2 (12%), TRPC6 (10%), and LAMB2 (9%). With various immunosuppressive or renoprotective therapies, remission of proteinuria in patients with unknown causative mutations was observed in 26% of patients, whereas only 5% of patients with monogenic disease-causing mutations exhibited complete remission. We assessed the genetic backgrounds of Japanese patients with severe proteinuria. The proportion of patients with gene defects was similar to that of other reports, but the disease-causing gene mutation frequency was considerably different.
Solid Sorbents as a Retrofit Technology for CO2 Removal from Natural Gas Under High Pressure and Temperature Conditions
Authors: Khraisheh Majeda , Almomani Fares , Walker Gavin 
Published: January 2020

AbstractThe capture of CO2 under high pressure and temperature is challenging and is required in a number for industrial applications including natural gas processing. In this work, we examine the use of benchmark hybrid ultraporous materials HUMs for their potential use in CO2 adsorption processes under high-pressure conditions, with three varying temperatures (283, 298 and 318 K). NbOFFOVE-1-Ni and SIFSIX-3-Ni were the selected HUMs given their established superior CO2 capacity under low pressure (0–1 bar). Both are microporous with highly ordered crystalline structures as compared to the mesoporous hexagonal silica (Santa Barbara Anhydrous-15 (SBA-15)). SBA-15 was previously tested for both low and high-pressure applications and can serve as a benchmark in this study. Sorbent characterization using XRD, SEM, FTIR and N2 adsorption were conducted to assure the purity and structure of the sorbents. TGA analysis were conducted to establish the thermal stability of the sorbents under various temperatures. High-pressure CO2 adsorption was conducted from 0–35 bar using magnetic suspension balance (Rubotherm). Although the SBA-15 had the highest surface (527 m3/g) are of the three adsorbents, the CO2 adsorption capacity (0.42 mmol/g) was an order of magnitude less than the studies HUMs with SIFSIX-3-Ni having 2.6 mmol/g, NbOFFIVE-1-Ni achieving 2.5 mmol/g at 298 K. Multistage adsorption isotherms were obtained at different pressures. In addition, results indicate that electrostatics in HUMs are most effective at improving isosteric heat of adsorption Qst and CO2 uptake. Higher temperatures had negative effect on adsorption capacity for the HUMs and SBA-15 at pressures between 7–9 bar. In SAB-15 the effect of temperature is reversed in what is known as a cross over phenomena.
The role of the dipole moment orientations in the crystallization tendency of the van der Waals liquids – molecular dynamics simulations
Authors: Koperwas Kajetan , Adrjanowicz Karolina , Grzybowski Andrzej , Paluch Marian 
Published: January 2020

AbstractComputer simulations of model systems play a remarkable role in the contemporary studies of structural, dynamic and thermodynamic properties of supercooled liquids. However, the commonly employed model systems, i.e., simple-liquids, do not reflect the internal features of the real molecules, e.g., structural anisotropy and spatial distribution of charges, which might be crucial for the behavior of real materials. In this paper, we use the new model molecules of simple but anisotropic structure, to studies the effect of dipole moment orientation on the crystallization tendency. Our results indicate that proper orientation of the dipole moment could totally change the stability behavior of the system. Consequently, the exchange of a single atom within the molecule causing the change of dipole moment orientation might be crucial for controlling the crystallization tendency. Moreover, employing the classical nucleation theory, we explain the reason for this behavior.
Role of surface microgeometries on electron escape probability and secondary electron yield of metal surfaces
Authors: Bajek D. , Wackerow S. , Zanin D. A. , Baudin L. , Bogdanowicz K. , Valdivieso E. Garcia-Tabares , Calatroni S. , Di Girolamo B. , Sitko M. , Himmerlich M. , Taborelli M. , Chiggiato P. , Abdolvand A. 
Published: January 2020

AbstractThe influence of microgeometries on the Secondary Electron Yield (SEY) of surfaces is investigated. Laser written structures of different aspect ratio (height to width) on a copper surface tuned the SEY of the surface and reduced its value to less than unity. The aspect ratio of microstructures was methodically controlled by varying the laser parameters. The results obtained corroborate a recent theoretical model of SEY reduction as a function of the aspect ratio of microstructures. Nanostructures - which are formed inside the microstructures during the interaction with the laser beam - provided further reduction in SEY comparable to that obtained in the simulation of structures which were coated with an absorptive layer suppressing secondary electron emission.
The crystallization behavior of the aqueous solution of CaCl2 salt in a drop and a layer
Authors: Misyura S. Y. 
Published: January 2020

AbstractNon-isothermal evaporation during crystallization of CaCl2 salt in a droplet and a thin layer on a hot wall has been investigated experimentally. The growth of salt crystal hydrates (CaCl2·2H2O) on the interface has been studied. It has been found that the change in the initial salt concentration leads to different crystallization rates. The crystallization rate on the droplet interface is many times lower than for a thin layer. The description of crystallization in the salt solution droplet should take into account the crystallization anisotropy, which is associated with the direction of crystallization. The crystallization rate along the contact line of the droplet is many times higher than in the direction of the droplet radius. For a long time of crystallization, the area of the crystal film (outside the drop) increases several times. Four characteristic modes of crystallization to a drop of salt solution have been distinguished. When modeling crystallization, it is necessary to take into account multiple changes in the growth rate of salt crystallohydrates over time, as well as the anisotropic nature of crystallization.
Elevated Coefficient of Variation in Total Fecal Bile Acids Precedes Diagnosis of Necrotizing Enterocolitis
Authors: Knapp Shannon , Kehring Allysa , Stepp Jennifer , Calton Christine M. , Gephart Sheila M. , Bandlamuri Sruti , Boyle Kate E. , Dietz Grey I. , Johnson Haeley , Romo Ryan E. , Spencer Mackenzie , Bedrick Alan D. , Halpern Melissa D. 
Published: January 2020

AbstractAccumulation of bile acids (BAs) may mediate development of necrotizing enterocolitis (NEC). Serial fecal samples were collected from premature infants with birth weight (BW) ≤ 1800 g, estimated gestational age (EGA) ≤ 32 weeks, and <30 days old prior to initiation of enteral feeding. Nine infants that developed Bell’s Stage ≥ II NEC were matched with control infants based on BW, EGA, day of life (DOL) enteral feeding was initiated and DOL of the first sample. From each subject, five samples matched by DOL collected were analyzed for BA levels and composition. Fifteen individual BA species were measured via LC-MS/MS and total BA levels were measured using the Diazyme Total Bile Acid Assay kit. No statistically significant differences in composition were observed between control and NEC at the level of individual species (p = 0.1133) or grouped BAs (p = 0.0742). However, there was a statistically significant difference (p = 0.000012) in the mean coefficient of variation (CV) between the two groups with infants developing NEC having more than four-fold higher mean CV than controls. Importantly, these variations occurred prior to NEC diagnosis. These data suggest fluctuations in total fecal BA levels could provide the basis for the first predictive clinical test for NEC.
Domain-mediated interactions for protein subfamily identification
Authors: Lee Heetak , Kim Inhae , Han Seong Kyu , Kim Donghyo , Kong Jungho , Kim Sanguk 
Published: January 2020

AbstractWithin a protein family, proteins with the same domain often exhibit different cellular functions, despite the shared evolutionary history and molecular function of the domain. We hypothesized that domain-mediated interactions (DMIs) may categorize a protein family into subfamilies because the diversified functions of a single domain often depend on interacting partners of domains. Here we systematically identified DMI subfamilies, in which proteins share domains with DMI partners, as well as with various functional and physical interaction networks in individual species. In humans, DMI subfamily members are associated with similar diseases, including cancers, and are frequently co-associated with the same diseases. DMI information relates to the functional and evolutionary subdivisions of human kinases. In yeast, DMI subfamilies contain proteins with similar phenotypic outcomes from specific chemical treatments. Therefore, the systematic investigation here provides insights into the diverse functions of subfamilies derived from a protein family with a link-centric approach and suggests a useful resource for annotating the functions and phenotypic outcomes of proteins.
Soil aggregates stability and storage of soil organic carbon respond to cropping systems on Black Soils of Northeast China
Authors: Zhou Meng , Liu Chunzhu , Wang Jie , Meng Qingfeng , Yuan Ye , Ma Xianfa , Liu Xiaobing , Zhu Yingxue , Ding Guangwei , Zhang Jizhou , Zeng Xiannan , Du Weiling 
Published: January 2020

AbstractMonoculture and improper management may reduce soil fertility and deteriorate soil structure in Black soils (Mollisols) of Northeast China. The experiment was carried out from 2015 to 2016 in Black Soils comprising five cropping systems: continuous corn (CC), soybean-corn rotation (SC), corn-soybean rotation (CS), fallow-corn (FC), and fallow-soybean (FS). Our results showed that CS and FS treatments significantly increased mean weight diameter (MWD) and fractal dimension (D) in mechanical stability aggregates (MSAs), and increased MWD and geometric mean diameter (GMD) in water-stable aggregates (WSAs) compared with CC treatment. These two treatments were also significantly increased water-stable aggregates stability rate (WSAR), but decreased percentage of aggregates destruction (PAD) than CC treatment. Meanwhile, CS and FS treatments exhibited a higher carbon accumulation than CC treatment in bulk soils. Soil organic carbon (SOC) concentration in WSA0.106-0.25,WSA2-5 mmand WSA0.5-1 mmhad a dominant effect on aggregate stability. Simutaneously, SOC in WSA>5 mmaffected SOC concentration in bulk soils. As a whole, the CS and FS treatments can increase the percentage of macro-aggregates, enhance aggregate stability, as well as increase SOC concentration in bulk soils and all soil aggregate sizes.
Recombinant thrombomodulin prevents acute lung injury induced by renal ischemia-reperfusion injury
Authors: Hayase Naoki , Doi Kent , Hiruma Takahiro , Matsuura Ryo , Hamasaki Yoshifumi , Noiri Eisei , Nangaku Masaomi , Morimura Naoto 
Published: January 2020

AbstractAcute kidney injury (AKI) complicated by acute lung injury has a detrimental effect on mortality among critically ill patients. Recently, a renal ischemia-reperfusion (IR) model suggested the involvement of histones and neutrophil extracellular traps (NETs) in the development of distant lung injury after renal IR. Given that recombinant thrombomodulin (rTM) has anti-inflammatory roles by binding to circulating histones, we aimed to clarify its effect on distant lung injury induced by AKI in a murine bilateral renal IR model. Both pretreatment and delayed treatment with rTM significantly decreased pulmonary myeloperoxidase activity, but they did not affect renal dysfunction at 24 h after renal IR. Additionally, rTM mitigated the renal IR-augmented expression of proinflammatory cytokines (tumor necrosis factor-α, interleukin-6, and keratinocyte-derived chemokine), and vascular leakage, as well as the degree of lung damage. Intense histone accumulation and active NET formation occurred in both the kidneys and the lungs; however, rTM significantly decreased the histone and NET accumulation only in the lungs. Administration of rTM may have protective impact on the lungs after renal IR by blocking histone and NET accumulation in the lungs, although no protection was observed in the kidneys. Treatment with rTM may be an adjuvant strategy to attenuate distant lung injury complicating AKI.
LINC00461/miR-4478/E2F1 feedback loop promotes non-small cell lung cancer cell proliferation and migration
Authors: Meng Qingxin , Liu Ming , Cheng Ruyi 
Published: January 2020

Abstract
Non-small cell lung cancer (NSCLC) is a prevalent subtype of lung cancer, whose mortality is high. Long non-coding RNAs (lncRNAs) have caught rising attentions because of their intricate roles in regulating cancerization and cancer progression. Long intergenic non-protein coding RNA 461 (LINC00461) has recently shown oncogenic potential in several cancers, but the function of LINC00461 in NSCLC remains to be investigated. Our study planned to unveil the regulatory role of LINC00461 in NSCLC. It was validated that LINC00461 was highly expressed in NSCLC tissues and cell lines and exhibited prognostic significance. Furthermore, LINC00461 expression in advanced stage was much higher than in early stage. Loss-of-function experiments suggested that LINC00461 knockdown impaired cell proliferation, migration, and epithelial-to-mesenchymal transition (EMT). Subcellular fractionation revealed the predominant location of LINC00461 in cytoplasm. Mechanistically, LINC00461 up-regulated E2F transcription factor 1 (E2F1) expression through sponging miR-4478. Besides, E2F1 bound to the promoter of LINC00461 to induce its transcription. Finally, rescue experiments verified that LINC00461 aggravated proliferation, migration, and EMT through targeting miR-4478/E2F1 axis. In consequence, the present study illustrated that LINC00461/miR-4478/E2F1 feedback loop promoted NSCLC cell proliferation and migration, providing a new prognostic marker for NSCLC.
Effects of emodin on inflammatory bowel disease-related osteoporosis
Authors: Luo Jing-sheng , Zhao Xinhua , Yang Yu 
Published: January 2020

Abstract
Inflammatory bowel diseases (IBD) are related to bone loss. Emodin can influence the activity and differentiation of osteoblasts and osteoclasts. However, few studies have shown the effects of emodin on IBD-induced bone damage. The aim of the present study was to investigate the role of emodin in IBD-induced osteoporosis in an animal model. An IBD model in Sprague Dawley male rats was established by administering 2.5% dextran sulfate sodium (DSS) in the drinking water. Emodin was administered orally (30 mg/kg body weight) every other day starting in the third week for 9 weeks. Blood, colon and bone samples were obtained for biomarker assays and histological analysis. Bone biomechanical properties, microCT, metabolic biomarkers and bone histological changes were analyzed. The bone mass was significantly decreased, and the bone biomechanical properties and bone microstructure parameters of IBD rats were significantly worse than those of control rats (P&lt;0.05). Tartrate resistant acid phosphatase staining also showed that the number of osteoclasts in bone in IBD rats were larger than that in bone in control rats. Emodin intervention abolished the changes in bone microstructure and biomechanical properties (P&lt;0.05) induced by IBD. Osteoclast formation and serum C-terminal cross-linked peptide (CTX) and tumor necrosis factor α (TNF-α) were also inhibited by emodin (P&lt;0.05). Emodin significantly abolished IBD-enhanced Traf6, NFATC1 and c-fos expression. Our data demonstrated that emodin suppresses IBD-induced osteoporosis by inhibiting osteoclast formation.
Protective role of peroxiredoxin-4 in heart failure
Authors: Ahmed Naseer , Naseem Masooma , Farooq Javeria 
Published: January 2020

Abstract
Recently, we have read with great interest the article published by Ibarrola et al. (Clin. Sci. (Lond.) (2018) 132, 1471–1485), which used proteomics and immunodetection methods to show that Galectin-3 (Gal-3) down-regulated the antioxidant peroxiredoxin-4 (Prx-4) in cardiac fibroblasts. Authors concluded that ‘antioxidant activity of Prx-4 had been identified as a protein down-regulated by Gal-3. Moreover, Gal-3 induced a decrease in total antioxidant capacity which resulted in a consequent increase in peroxide levels and oxidative stress markers in cardiac fibroblasts.’ We would like to point out some results stated in the article that need further investigation and more detailed discussion to clarify certain factors involved in the protective role of Prx-4 in heart failure.
A young patient with type 2 diabetes associated non-alcoholic steatohepatitis, liver cirrhosis, and hepatocellular carcinoma
Authors: Michel Maurice , Kalliga Eva , Labenz Christian , Straub Beate K. , Wörns Marcus-Alexander , Galle Peter R. , Schattenberg Jörn M. 
Published: January 2020

AbstractThe rising prevalence of the metabolic syndrome has led to an increase of non-alcoholic fatty liver disease (NAFLD), and its progressive-inflammatory form called non-alcoholic steatohepatitis (NASH). In recent years, NAFLD and NASH have become major risk factors for developing liver cirrhosis and hepatocellular carcinoma (HCC). In this case, we report a 46-year-old patient with type 2 diabetes mellitus and metabolic comorbidities including obesity and arterial hypertension, who was referred because of rising liver enzymes. After clinical and diagnostic evaluation, the patient was diagnosed with NASH-associated liver cirrhosis, Child-Pugh stage B. A normal blood sugar level was difficult to achieve, and the patient presented with consistently elevated HbA1c-levels irresponsive to insulin therapy. Due to the underlying liver cirrhosis, the patient was enrolled in the HCC-surveillance program. Sonography during follow up showed a focal lesion. On magnetic resonance imaging (MRI), the diagnosis of HCC (BCLC stage A) was confirmed based on typical contrast enhancement and portal-venous wash-out. The patient was evaluated for liver transplantation with a labMELD of 17, and an intermittent therapy with TACE was initiated. Only 2 months after liver transplantation, the patient developed severe and lethal complications. Overall, this case highlights the different medical issues of patients with metabolic syndrome developing a chronic liver disease. In this patient, a rapid progression from NASH-associated liver cirrhosis to HCC was seen, and therefore highlights the importance of close surveillance to identify and treat potential risk factors early in the course of the disease.
Hip Adduction and Abduction Torque-angle Curve Characterization of Speed Skating Athletes
Authors: Menegaldo Luciano Luporini , Aragão Lyon , Matta Thiago , de Oliveira Liliam Fernandes 
Published: January 2020

Abstract
Speed skating is a cyclic sport which involves the hip abductor muscles, impelling the participant forwards, and adductor muscles, in the recovery phase and decelerating the abduction movement eccentrically. This paper has the objective of describing and comparing the abduction/adduction torque-angle curves of speed skating athletes (N=10) with a group of non-practitioners young participants (N=10). Both groups presented similar peak torques and electromyography patterns for tensor fascia lata, gluteus medius, long adductor and adductor magnus. However, athletes showed higher torque-angle curve integral and abduction and adduction peak torques at different hip angles than the control group. These findings suggest an adaptation of their musculotendon actuators and a better capacity to generate mechanical work and power during a propulsion-recovery cycle.
Symptomatic Postoperative Spinal Epidural Hematoma after Spinal Decompression Surgery: Prevalence, Risk Factors, and Functional Outcome
Authors: Hohenberger Christoph , Zeman Florian , Höhne Julius , Ullrich Odo-Winfried , Brawanski Alexander , Schebesch Karl-Michael 
Published: January 2020

Abstract
Objective Postoperative spinal epidural hematoma (pSEH) with symptomatic compression of nervous structures after spinal decompression surgery is a rare complication. Delayed evacuation may result in severe neurologic impairment. We present a large single-center analysis of the prevalence, potential risk factors, and functional recovery after pSEH.
Methods A retrospective review of our institutional database of spinal decompression surgery over 15 years yielded 6,024 consecutive patients. A total of 42 patients who had undergone surgical revision due to postoperative neurologic deterioration or intractable radiating pain and radiographically confirmed pSEH were allocated to the pSEH group. A matched 3:1 control group was formed (126 patients with the same surgical procedure, same year, same sex, and similar age). Charts, surgical reports, and radiographic data were reviewed for demographics, duration of symptoms, history of medical treatment, medication, comorbidities, radiographic extension, surgical strategy, and pre- and postoperative neurologic performance. Median follow-up was 3 months. Risk factors for pSEH, complete recovery, and recovery of neurologic symptoms were analyzed with univariable and multivariable logistic regression models.
Results The prevalence of pSEH in this population was 0.69% (n = 42) with these locations: 7 of 1,284 (0.54%) cervical, 1 of 774 (0.12%) thoracic, and 34 of 3,966 (0.85%) lumbar. Use of anticoagulants (p = 0.003), pathologic coagulation values in the preoperative blood test (p = 0.034), and cigarette smoking (p = 0.003) were identified as independent risk factors of pSEH. Surgery in more than one level showed a trend toward an increased risk of pSEH. Pain as the only symptom (p = 0.0001) was a significant predictor of complete recovery. Patients symptomatic with paraplegia (p = 0.026) had a significantly higher risk of a poor neurologic outcome without full recovery of neurologic symptoms.
Conclusion The prevalence of pSEH was lower than previously reported incidences. Use of anticoagulants, pathologic coagulation values, and cigarette smoking were identified as independent risk factors of pSEH. Functional outcome was related to the duration between hematoma evacuation and the clinical presentation of symptomatic pSEH.