bluuebunny/crossref_metadata_2025_split huggingface dataset:
An affinity-matured human monoclonal antibody targeting fusion loop epitope of dengue virus within vivotherapeutic potency
Authors: Kotaki Tomohiro , Kurosu Takeshi , Grinyo Ariadna , Davidson Edgar , Churrotin Siti , Okabayashi Tamaki , Puiprom Orapim , Mulyatno Kris Cahyo , Sucipto Teguh Hari , Doranz Benjamin J. , Ono Ken-ichiro , Soegijanto Soegeng , Kameoka Masanori 
Published: October 2020

AbstractDengue virus (DENV), from the genusflavivirusof the familyflaviviridae, causes serious health problems globally. Human monoclonal antibodies (HuMAb) can be used to elucidate the mechanisms of neutralization and antibody-dependent enhancement (ADE) of DENV infections, leading to the development of a vaccine or therapeutic antibodies. Here, we generated eight HuMAb clones from an Indonesian patient infected with DENV. These HuMAbs exhibited the typical characteristics of weak neutralizing antibodies including high cross-reactivity with other flaviviruses and targeting of the fusion loop epitope (FLE). However, one of the HuMAbs, 3G9, exhibited strong neutralization ability (NT50< 0.1 µg/ml) and possessed a high somatic hyper-mutation rate of the variable region, indicating affinity-maturation. Administration of this antibody significantly improved the survival rate of interferon-α/β/γ receptor knockout C57BL/6 mice after a lethal DENV challenge. Additionally, Fc-modified 3G9 molecules that had lost their in vitro ADE activity showed significantly enhanced therapeutic potencyin vivoand competed strongly with an ADE-prone antibodyin vitro. Taken together, the affinity-matured FLE-targeting antibody 3G9 exhibits several promising features for therapeutic application including a low NT50value, potential for pan-flavivirus infection treatment, and suppression of ADE. This study demonstrates the therapeutic potency of affinity-matured FLE-targeting antibodies.
Investigation of RNA metabolism through large-scale genetic interaction profiling in yeast
Authors: Decourty Laurence , Malabat Christophe , Frachon Emmanuel , Jacquier Alain , Saveanu Cosmin 
Published: October 2020

AbstractGene deletion and gene expression alteration can lead to growth defects that are amplified or reduced when a second mutation is present in the same cells. We performed 154 genetic interaction mapping (GIM) screens with mutants related with RNA metabolism and measured growth rates of about 700 000Saccharomyces cerevisiaedouble mutant strains. The screens used the gene deletion collection in addition to a set of 900 strains in which essential genes were affected by mRNA destabilization (DAmP). To analyze the results we developed RECAP, a strategy that validates genetic interaction profiles by comparison with gene co-citation frequency, and identified links between 1 471 genes and 117 biological processes. To validate specific results, we tested and confirmed a link between an inositol polyphosphate hydrolase complex and mRNA translation initiation. Altogether, the results and the newly developed analysis strategy should represent a useful resource for discovery of gene function in yeast.
A gradual backward shift of dopamine responses during associative learning
Authors: Amo Ryunosuke , Yamanaka Akihiro , Tanaka Kenji F. , Uchida Naoshige , Watabe-Uchida Mitsuko 
Published: October 2020

AbstractIt has been proposed that the activity of dopamine neurons approximates temporal difference (TD) prediction error, a teaching signal developed in reinforcement learning, a field of machine learning. However, whether this similarity holds true during learning remains elusive. In particular, some TD learning models predict that the error signal gradually shifts backward in time from reward delivery to a reward-predictive cue, but previous experiments failed to observe such a gradual shift in dopamine activity. Here we demonstrate conditions in which such a shift can be detected experimentally. These shared dynamics of TD error and dopamine activity narrow the gap between machine learning theory and biological brains, tightening a long-sought link.
A critical residue in the α1M2-M3 linker regulating GABAAreceptor pore gating by diazepam
Authors: Nors Joseph W. , Gupta Shipra , Goldschen-Ohm Marcel P. 
Published: October 2020

AbstractBenzodiazepines (BZDs) are a class of widely prescribed psychotropic drugs. Their anxiolytic and sedative effects are conferred by modulating the activity of GABAAreceptors (GABAARs), which are the primary inhibitory neurotransmitter receptors throughout the central nervous system. However, the physical mechanism by which BZDs exert their effects on the receptor is poorly understood. In particular, BZDs require coapplication with an agonist to effectively open the channel pore, making it difficult to dissect whether the drug has altered either agonist binding or channel gating as these two processes are intimately coupled. To isolate effects on gating we used a spontaneously active gain of function mutant (α1L9’Tβ2γ2L) that is directly gated by BZDs alone in the absence of agonist. In the α1L9’T background we explored effects of alanine substitutions throughout the α1M2-M3 linker on modulation of the channel pore by the BZD positive modulator diazepam (DZ). The M2-M3 linker is known to be an important element for channel activation. Linker mutations generally impaired unliganded pore opening, indicating that side chain interactions are important for channel gating in the absence of bound agonist. All but one mutation had no effect on the transduction of chemical energy from DZ binding to pore gating. Strikingly, α1V279A doubles DZ’s energetic contribution to gating, whereas larger side chains at this site do not. In a wild-type background α1V279A enhances DZ-potentiation of currents evoked by saturating GABA, consistent with a direct effect on the pore closed/open equilibrium. Our observations identify an important residue regulating coupling between the BZD site and the pore gate, thereby shedding new light on the molecular mechanism of a frequently prescribed class of psychotropic drugs.
Omnitemporal choreographies of IP3R and all five STIM/Orai underlie the complexity of mammalian Ca2+signaling
Authors: Emrich Scott M. , Yoast Ryan E. , Xin Ping , Arige Vikas , Wagner Larry E. , Hempel Nadine , Gill Donald L. , Sneyd James , Yule David I. , Trebak Mohamed 
Published: October 2020

SummaryInvertebrates express one endoplasmic reticulum (ER)-resident Ca2+-sensing stromal-interaction molecule (Stim) and one Orai plasma membrane channel protein. Stim conveys store depletion to Orai, mediating the evolutionarily conserved Ca2+release-activated Ca2+(CRAC) current. The crucial role of their vertebrate homologues, STIM1 and Orai1 in mediating CRAC activity in mammals is well-established. However, mammals possess two STIM and three Orai isoforms and the choreography of their interactions under physiological receptor activation is unknown. We show that the five mammalian STIM1/2 and Orai1/2/3 isoforms have non-redundant functions. Yet, all five isoforms are always required together to ensure the graded diversity of mammalian Ca2+signaling events in response to the full spectrum of agonist strengths. Receptor-activated Ca2+signaling across the range of stimulus intensities requires functional interactions between not only STIM1/2 and Orai1/2/3, but also IP3R, ensuring that receptor-mediated Ca2+release is precisely tailored to Ca2+entry and activation of nuclear factor of activated T-cells (NFAT). This is orchestrated by two interdependent and counterbalancing paradigms: the N-termini Ca2+-binding ER-luminal domains ofunactivatedSTIM1/2 inhibit IP3R-evoked Ca2+release. Gradual increase in agonist intensity leads to gradual STIM1/2 activation and relief of IP3R inhibition. Concomitantly, the cytosolic C-termini ofactivatedSTIM1/2 differentially interact with Orai1/2/3 proteins as agonist intensity increases. Thus, coordinated and omnitemporal functions of all five STIM/Orai proteins and IP3Rs at the ER-lumen and cytosol translate the strength of agonist stimulation to precise levels of Ca2+release, Ca2+entry and NFAT induction, ensuring the diversity and fidelity of complex mammalian Ca2+signaling.HighlightsAll five STIM/Orai and IP3R are always required together in mammalian Ca2+signallingUnactivated STIM1/2 inhibit IP3R and activated STIM1/2 cooperatively activate Orai1/2/3STIM1 contribution increases and that of STIM2 decreases as agonist intensifiesGraded IP3R disinhibition and Orai activation tailor receptor activity to NFAT induction
Multinationality and performance effects: an integrated theory of new patterns of multinationality and home political influence
Authors: Yen I-Fan , Lin Hsin-Mei 
Published: October 2020

PurposeThis paper aims to develop an integrated perspective on the relationship between multinationality and performance in the outward foreign direct investment (OFDI) of Chinese firms. The study not only represents contrasting OFDI patterns – namely, born global-natured multiple synchronous foreign investments versus conventional internationalization process (IP)-natured steady increasing foreign investments – but also contributes to understanding the extent to which explanations of home political influence need to be rooted within the general theory of multinationality.Design/methodology/approachBy testing a comprehensive panel observation of 8,635 OFDI projects from 1991-2016 in China, this study found that multinationality with the new pattern of multiple synchronous OFDIs has a superior performance effect compared with the conventional pattern of steady increasing OFDIs.FindingsThis study also finds a positive relationship between multinationality (international diversification and home political influence) and the performance effect with the new pattern of multiple synchronous OFDIs, as well as a partial positive relationship between multinationality and the performance effect with the conventional pattern of steady increasing OFDIs.Research limitations/implicationsThe study extends the understanding of the performance effects of Chinese multinational enterprises, which may benefit more from the new pattern of multiple synchronous OFDIs than from the conventional pattern of steady increasing OFDIs when the home-country institution is strongly positioned.Originality/valueThis paper concludes that multinationality needs an integrated framework that accounts for the new pattern of OFDI and the influence of diversification and home politics, particularly for the emerging country, China.
Effective strategies for developing retirement village public – private partnership
Authors: Osei-Kyei Robert , Tam Vivian , Ma Mingxue 
Published: October 2020

Purpose
The growth in ageing population globally has led to the increase in demand for retirement or aged care homes. Adopting public–private partnership (PPP) in the global retirement village market has become the new approach to address some of the emerging challenges. This paper aims to explore and analyse the critical success factors (CSFs) for the adoption of PPP in the global retirement village market.

Design/methodology/approach
An empirical questionnaire survey was conducted with experienced practitioners in the global PPP and retirement village markets. Mean score analysis, normalization range method, Kendall’s coefficient of concordance and factor analysis were used for analysis.

Findings
Results show that out of the 27 CSFs identified, the most significant ones in developing PPP retirement village projects are “the age-friendly design of villages”, “appropriate location of PPP retirement village”, “reliable and accessible health and physical facilities” and “effective social inclusion and integration in villages”. Further analysis shows that the 27 CSFs can be grouped into 7 major factor groupings, namely, “effective project monitoring”, “financial support”, “social integration”, “effective contractual arrangement between parties”, “government commitment and support”, “sustainable design of village” and “effective payment structure”.

Originality/value
The outputs of this study will adequately inform retirement village developers, retirement village stakeholders and local government authorities of the best practices they should put in place to ensure the sustainable growth of the global retirement village market.
Being a student in the social media era: exploring educational affordances of an ePortfolio for managing academic performance
Authors: Alexiou Aikaterini , Paraskeva Fotini 
Published: October 2020

PurposeUndergraduate students often find it difficult to organize their learning activities and manage their learning. Also, teachers need dynamic pedagogical frameworks and learning technologies for supporting learners to advance their academic performance. The purpose of this paper is to investigate the effect of an ePortfolio intervention on self-regulated learning (SRL cognitive, affective, behavioral and contextual processes) and academic achievement.Design/methodology/approachFor the purposes of this study, an ePortfolio was designed and implemented based on SRL. The ePortfolio-based self-regulated learning approach (ePSRL) system encompasses the merits of a social networking platform and the functionalities of a learning management system. The participants were 123 university students (38 females and 85 males) at a computer science department. Students were randomly divided into two groups, the experimental and the control group.FindingsThe results of the study indicate that there is a significant increase of the means across SRL processes between the perceptions in the experimental and the control group. The implementation of the ePSRL approach as a learning module for undergraduate students could enable learners to manage their learning processes, transform their behavior into measurable learning outcomes and foster their academic performance.Originality/valueThis paper considers the importance of SRL and ePortfolios. Also, highlights the need of providing technology enhanced training courses and interventions to undergraduate students for supporting them to thrive during their academic studies. Thus, it proposes a set of educational affordances and practical guidelines that can be used by practitioners, instructional designers and educators in higher education as well as in vocational education and training institutions.
The impact of institutional voids on risk and performance in base-of-the-pyramid supply chains
Authors: Rehman Attique ur , Jajja Muhammad Shakeel Sadiq , Khalid Raja Usman , Seuring Stefan 
Published: October 2020

PurposeBase-of-the-pyramid (BoP) markets are frequently characterized by institutional voids. However, it remains unclear how institutional voids impact corporate and supply chain risk and performance. This intersection will be analyzed in this paper.Design/methodology/approachThis paper presents a systematic literature review of 94 BoP papers published between 2004 and 2019 in peer-reviewed, English-language journals available on Scopus. Drawing upon established frameworks for examining institutional voids, supply chain risks and BoP performance, frequency, and contingency analyses are conducted. Contingencies are established to provide insights into the associations between different constructs from the selected frameworks.FindingsSupply chain risks are pervasive in the BoP discourse, especially when BoP markets are characterized by institutional voids. The frequency analysis of the constructs suggests that the key supply chain risks discussed in the BoP literature include social risk, credit risk, product market and operating uncertainties, knowledge and skill biases and decision-maker risks due to bounded rationality. The contingency analysis suggests that institutional voids are associated with supply chain risks that affect performance.Research limitations/implicationsA theoretical framework aligning three research streams in the context of BoP calls for future studies to test the causality of highlighted constructs that are significantly associated. The analysis is confined to the constructs that are taken into account based on specific conceptual frameworks.Practical implicationsThe study provides practitioners with a framework to manage supply chain risks in BoP-related firms to enhance firm performance. Managers can use key dimensions of supply chain risk, such as the product market, the input market and operating uncertainties, to evaluate performance in the BoP context.Originality/valueSpecifically, this research has strengthened the inquiry of supply chain risks in the presence of institutional voids that may have an impact on firm performance
The influence of the COVID-19 pandemic on the digital transformation of work
Authors: Nagel Lisa 
Published: October 2020

PurposeThis study investigates whether the COVID-19 pandemic has led to an acceleration of the digital transformation in the workplace.Design/methodology/approachThis study is based on a survey conducted during the COVID-19 pandemic from March to April 2020 on the crowdsourcing platform Amazon Mechanical Turk.FindingsThe findings show an increase of people working from home offices and that many people believe that digital transformation of work has accelerated in response to the COVID-19 pandemic. People who noted this acceleration can imagine working digitally exclusively in the future. Moreover, the importance of traditional jobs as a secure source of income has decreased, and digital forms of work as a secure source of income have increased because of the COVID-19 pandemic. Workers believe that digital work will play a more important role as a secure source of income in the future than traditional jobs.Research limitations/implicationsBecause the survey was conducted online, respondents may have had a certain affinity for digital work.Originality/valueThis study assesses the consequences of the COVID-19 pandemic on the future of work, showing that changes in the perception of digital transformation and the willingness to work exclusively in a digital manner have arisen as result of the COVID-19 pandemic. To estimate the long-term consequences of the pandemic on the digitisation of work, research that includes macroeconomic consequences in its forecast is necessary.
Swimming in a sea of uncertainty – business, governance and the coronavirus (COVID-19) pandemic
Authors: Hopper Trevor 
Published: October 2020

Purpose
The purpose of this paper is to examine accounting-related issues that have emerged during the coronavirus (COVID-19) pandemic.

Design/methodology/approach
The discussion is based on personal experience and observation of events and media reports in the UK, the USA and New Zealand.

Findings
Many problems that have emerged have varied owing to differences in the national governance of each country regarding the emphasis their governments and businesses have placed on short-run financialisation policies; the comprehensiveness of their information and control systems; their leadership and whether their discourse accords with events and expert advice; their degree of accountability and concern for public wellness; and the need to consider new taxation policies to meet the costs of the pandemic.

Research limitations/implications
This paper is based on personal observations owing to the restrictions on research access during the pandemic, and thus it reflects the author’s political opinions and beliefs.

Practical implications
This paper outlines areas where accounting could and has addressed the issues examined and recommends greater adoption globally of policies and systems designed to meet the United Nation’s sustainable development goals.

Social implications
The social implications are vast for they extend to major issues concerning preserving the planet, its species, humankind and enhanced democratic processes for civil society and developing countries.

Originality/value
A comparative analysis and evaluation of nations' control of of the coronavirus (COVID_19) pandemic. This lies in the eyes of the beholder.
Institutions, entrepreneurial adaptation, and the legal form of the organization
Authors: Khurana Indu , Krichevskiy Dmitriy , Dempster Gregory , Stimpson Sean 
Published: October 2020

PurposeThis paper aims to examine how economic freedom impacts the initial choice of legal structure for startup firms. The authors do this by first exploring whether economic freedom is an essential determinant of the initial legal form of organization (LFO). The authors then explore the impact of economic freedom on firms' choice of changing their initial legal structure over time and how this change impacts their survival rate.Design/methodology/approachThe authors employ a multinomial logistic regression model to measure the initial determinants of LFO by utilizing an eight-year panel data set of 4,928 startups in the USA through the Kauffman firm survey and merge it with the Economic Freedom in North American index from the Fraser Institute. The authors then employ a logistic regression model to examine the determinants facilitating a change in legal structure over time.FindingsThe results show that economic freedom is a significant determinant in the choice of legal structure. The findings also report that the majority of startups do not change their legal form, but of those that do change the legal structure show a higher survival rate.Research limitations/implicationsMajor limitations are the size of the data and the nature of somewhat limited economic freedom differences with the USA. More nuanced measures of economic freedom would be highly desirable.Practical implicationsPolicymakers should take note that limited red tape, smoothly working labor markets and straightforward processes for changes of legal structures of organizations would improve survival and growth odds for entrepreneurs.Originality/valueDrawing on the theory of institutions, the authors attempt to bridge a gap in the literature by explicitly analyzing the determinants of the legal structure in startups in light of economic freedom. Institutional factors do not work in isolation; therefore, the authors also employ traditional entrepreneur-specific variables that affect the choice of legal structure in addition to the institutional framework.
Cross-disciplinary innovations by Taiwanese manufacturing SMEs in the context of Industry 4.0
Authors: Chen Chun-Liang 
Published: October 2020

PurposeThe aim of this paper is to explore how small and medium enterprises (SMEs) in Taiwan employ technology to participate in global supply chains so as to respond to the Fourth Industrial Revolution.Design/methodology/approachThis study chose four small to medium textile SMEs using qualitative exploratory multiple case studies to examine their participation in the global value chain (GVC) and under the context of Industry 4.0.FindingsThis study proffered a strategic model for the innovative integration of textile manufacturing companies and cultural content industry into the global market. The results identified four types of cross-disciplinary value creation strategies by Industry 4.0-driven technology and cultural content infusion: enhancing digital product display capabilities, integrating cultural content design and online marketing, creative brand marketing with cyber-physical channel integration and emotional marketing incorporated with smart services.Originality/valueThe author proposed the following cross-disciplinary value creation strategies for clothing SMEs in Taiwan: (1) enhancing digital product display capabilities, (2) integrating cultural content design and online marketing, (3) creative brand marketing with cyber-physical integration and (4) emotional marketing incorporated with smart services. Using these strategies, SMEs can incorporate cultural and lifestyle aspects into products and services and embed themselves in the global marketing links of GVCs.