bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modified IVC Clamping Technique in the Left Radical Nephrectomy and Mayo II-III IVC Thrombectomy: A Preliminary Experience
Authors: Chen Zhigang , Yang Bin , Ge Liyuan , Zhang Fan , Tian Xiaojun , Huang Yi , Wang Guoliang , Liu Cheng , Zhang Shudong , Ma Lulin 
Published: November 2020

Abstract
Background: To present our initial experiences on the left radical nephrectomy (RN) and Mayo II-III IVC thrombectomy (IVCTE) using modified inferior vena cava (IVC) clamping technique. Methods: From November 2016 to July 2018, eight left renal cell carcinoma (RCC) patients with inferior vena cava tumor thrombus (IVCTT) underwent retroperitoneal laparoscopic RN and IVCTE using the modified IVC clamping technique. During the IVCTE, the infrarenal IVC, right renal artery, right renal vein were clamped sequentially, then the cephalic IVC of the tumor thrombus was clamped immediately after the thrombus was removed.Results: According to the preoperative plans, all 8 operations were completed successfully without perioperative mortality. Median operative time was 438 min (343-573 min). Median IVC blocking time was 18 min (12-28min), and median warm ischemia time (WIT) for the right kidney was 19min (14-28min). Median estimated intraoperative blood loss was 1107mL (50-6000 ml). Some 50% of patients required an intraoperative blood transfusion. Median length of hospital stay was 12.9 days (6-39). Early postoperative complications occurred in 2 cases, 1 was Clavien class II, another was Clavien class IVa. All 8 patients were followed up continually with a median follow-up period of 16 months (5-25 months). During the mean follow-up period, three patients developed metastatic disease.Conclusions: Modified IVC clamping technique, which is feasible and safe for experienced surgeons in selected patients, can simplify the procedures left RN and Mayo II-III IVCTE.
Healthcare Workers Preparedness for COVID-19 Pandemic in the Occupied Palestinian Territory: A Cross-sectional Survey
Authors: Alser Osaid , Alghoul Heba , Alkhateeb Zahra , Hamdan Ayah , Albarqouni Loai , Saini Kiran 
Published: November 2020

Abstract
Background: The coronavirus disease 19 (COVID-19) pandemic threatens to overwhelm the capacity of a vulnerable healthcare system in the occupied Palestinian territory (oPt). Sufficient training of healthcare workers (HCWs) in managing COVID-19 and the provision of personal protective equipment (PPE) are essential in allowing oPt to mount a credible response to the crisis.Methods: A cross-sectional study was conducted using a validated online questionnaire between March 30, 2020 and April 12, 2020. Primary outcomes were availability of PPE and HCWs preparedness in oPt for COVID-19 pandemic. Secondary outcome was regional and hospital differences in oPt in terms of availability of PPE and HCWs preparedness.Results: Of 138 respondents, only 38 HCWs (27.5%) always had access to facemasks and 15 (10.9%) for isolation gowns. Most HCWs did not find eye protection (n=128, 92.8%), N95 respirators (n=132, 95.7%), and face shields (n=127, 92%) always available. Compared to HCWs in West Bank, those in the Gaza Strip were significantly less likely to have access to alcohol sanitizers (p=0.03) and gloves (p <0.001). On average, governmental hospitals were significantly less likely to have all appropriate PPE than non-governmental institutions (p = 0.001). Only 16 (11.6%) surveyed felt confident in dealing with a potential COVID-19 case, 57 (41.3%) having received any COVID-19-related training, and 57 (41.3%) not having a local hospital protocol. Conclusion: HCWs in oPt are underprepared and severely lacking adequate PPE provision. The lack of PPE provision will exacerbate spread of COVID-19 and deepen the crisis, whilst putting HCWs at risk.
CagA sequence differences and proteome profiles between East Asian and Western H. pylori strains
Authors: Zhou Jianjiang , Zhao Yan , Xie Yang , Xiong Lin , Quan Xinying , Wang Qin-Rong , Wang Wen-Ling , Gin Feng , Zhang Qi-Fang , Liao De-Zhong , Zellmer Lucas 
Published: November 2020

Abstract
Background

The cytotoxin-associated gene A protein (CagA), an effector protein of Helicobacter pylori (H. pylori), was the first identified bacterium oncoprotein. Based on its sequence characteristics, H. pylori has been classified into East Asian and Western strains. We hypothesized that the differences in structure of CagA and proteomic profiles between East Asian and Western H. pylori strains are the primary cause of the differential clinical outcomes of H. pylori infection.
Results

In this study, we isolated 27 H. pylori strains from gastric mucosa of Chinese patients with gastric diseases and revealed that the Western CagA has more variation in its EPIYA motifs than East Asian CagA. This result was further confirmed via analyzing the CagA sequences of 150 H. pylori strains from GenBank. More importantly, we detected the deletion or partial deletion of 13 amino acids in CagA in all East Asian strains but not in Western strains. iTRAQ -based proteomic analysis showed that the CagA protein related to cytotoxicity was highly expressed in Western strains, while urease-associated proteins UreA and UreH, flagellin related proteins FlaA, FlgE and FlhA, and cell division proteins FtsZ and FtsI were highly expressed in East Asian strains. These proteins were associated with the colonization, motility, and viability potential of H. pylori in the human stomach and were clustered into an interaction network.
Conclusions

This study provides significant, unreported differential sequences of CagA and proteomic profiles between East Asian and Western H. pylori, which maybe serves as promising new targets to ascertain the pathogenesis of H. pylori.
HPP-induced transcriptome response during recovery of Listeria monocytogenes
Authors: Duru Ilhan Cem , Bucur Florentina Ionela , Andreevskaya Margarita , Nikparvar Bahareh , Ylinen Anne , Grigore-Gurgu Leontina , Rode Tone Mari , Crauwels Peter , Laine Pia , Paulin Lars , Løvdal Trond , Riedel Christian U. , Bar Nadav , Borda Daniela , Nicolau Anca Ioana , Auvinen Petri 
Published: November 2020

Abstract
Background
High-pressure processing (HPP) is a commonly used technique in the food industry to inactivate pathogens, including L. monocytogenes. It has been shown that L. monocytogenes is able to recover from HPP injuries and can start to grow again during long-term cold storage. To date, the gene expression profiling of L. monocytogenes during HPP damage recovery at cooling temperature has not been studied. In order identify key genes that play a role in recovery of the damage caused by HPP treatment, we performed RNA-sequencing (RNA-seq) for two L. monocytogenes strains (barotolerant RO15 and barosensitive ScottA) at nine selected time points (up to 48 h) after treatment with two pressure levels (200 and 400 MPa).
Results
The results showed that a general stress response was activated by SigB after HPP treatment. In addition, the phosphotransferase system (PTS), protein folding, and cobalamin biosynthesis were the most upregulated genes during HPP damage recovery. We observed that cell-division-related genes (divIC, dicIVA, ftsE, and ftsX) were downregulated. By contrast, peptidoglycan-synthesis genes (murG, murC, and pbp2A) were upregulated. This indicates that cell-wall repair occurs as a part of HPP damage recovery. We also observed that prophage genes, including anti-CRISPR genes, were induced by HPP. Interestingly, a large amount of RNA-seq data (up to 85%) was mapped to Rli47, which is a non-coding RNA that is upregulated after HPP. Thus, we predicted that Rli47 plays a role in HPP damage recovery in L. monocytogenes. Moreover, gene-deletion experiments showed that amongst peptidoglycan biosynthesis genes, pbp2A mutants are more sensitive to HPP.
Conclusions
We identified several genes and mechanisms that may play a role in recovery from HPP damage of L. monocytogenes. Our study contributes to new information on pathogen inactivation by HPP.
Evaluating Deep Learning EEG-Based Anxiety Classification in Adolescents with Autism for Breathing Entrainment BCI
Authors: Penchina Brian , Sundaresan Avirath , Cheong Sean , Grace Victoria , Valero-Cabré Antoni , Martel Adrien 
Published: November 2020

Abstract
Anxiety is one of the most common comorbidities in youth with autism spectrum disorder (ASD), severely limiting academic opportunities and overall quality of life. In the present study, we compared several machine learning classifiers, namely support vector machine (SVM) and deep learning methods, in order to evaluate the feasibility of an EEG-based BCI for the real-time assessment and mitigation of anxiety through a closed-loop adaptation of respiration entrainment. We trained a total of eleven subject-dependent models—four with conventional BCI methods and seven with deep learning approaches—on the EEG of neurotypical (n=5) and ASD (n=8) participants performing alternating blocks of mental arithmetic stress induction, guided and unguided breathing. We propose a multiclass two-layer LSTM RNN deep learning classifier capable of identifying anxious states from ongoing EEG with an overall accuracy of 93.27%. Our study is the first to successfully apply an LSTM RNN classifier to identify anxious states from EEG in both neurotypical adolescents and adolescents with ASD.
Long Non-Coding RNA MPNCR is a Negative Regulator of Bovine Mammary Epithelial Cell Proliferation
Authors: Wang Lixian , Zhang Yuelang , Liu Lihua , Li Fang , Zhang Xiaolan , Zhang Weidong , Khatib Hasan , Wang Xin 
Published: November 2020

Abstract
BackgroundNumerous studies have reported that many long non-coding RNAs (lncRNAs) may play multiple essential roles during mammary gland development; however, little is known about their functions and regulatory mechanism.ResultsIn this study, we confirmed a novel lncRNA, MPNCR, that was highly expressed during lactation compared to the dry period in dairy cattle. The potential biological and regulatory functions of MPNCR were explored in bovine mammary epithelial cells (BMECs). The MTT and EdU assays revealed that lncRNA MPNCR significantly suppressed cell viability and proliferation of BMECs. The subcellular localization indicated that MPNCR had a potential function as a competing endogenous RNA (ceRNA) to regulate the proliferation of BMECs. Bioinformatics analysis showed that lncRNA MPNCR had two binding sites for miR-31, further confirmed by the dual-luciferase report assay. The RT-qPCR result showed that the expression of miR-31 was downregulated following the overexpression of MPNCR in BMECs. Furthermore, the expression of MPNCR was significantly inhibited by miR-31 overexpression and upregulated by miR-31 inhibition. Cell viability and proliferation were significantly suppressed or promoted with the transfection of miR-31 inhibitor or miR-31 mimic into BMECs, respectively, whereas rescue with MPNCR reduced the proliferation of BMECs. The dual-luciferase report assay showed an interaction between miR-31 and the 3' UTR of the target gene CAMK2D. RT-qPCR and western blot further showed that the expression of CAMK2D was significantly increased with the overexpression of miR-31, while its expression was significantly suppressed with the inhibition of miR-31, indicating CAMK2D was a target of miR-31.ConclusionTaken together, these results demonstrate the function and regulatory mechanism of lncRNA MPNCR on the proliferation of BMECs, and that its regulation may contribute to bovine mammary cell population management.
PCAT-1 Expression is Associated With The Prognosis in Prostate Cancer.
Authors: Li Sheng , Ruan Xiaolan , Liu Tongzu 
Published: November 2020

Abstract
Purpose: In the study, we aimed to estimate the prognostic significance of PCAT-1 in patients with prostate cancer (PCa).Methods: The expression of PCAT-1 in paired PCa tissues and normal controls was examined via quantitative real-time polymerase chain reaction (qRT-PCR). The influence of PCAT-1 level on clinical features was assessed using Chi-square test. The survival curves were plotted to estimate the prognosis of patients. And the Cox analysis was carried out to find promising predictive factors for patients.Results: The expression level of PCAT-1 in PCa tissues was significantly elevated compared with the adjacent normal control (P<0.0001). The increased expression of PCAT-1 was affected by high Gleason score (P=0.017), positive serum PSA (P=0.011) and advanced TNM stage (P=0.003). The Kaplan-Meier survival curves showed that the overall survival rate of patients with high PCAT-1 expression was significantly lower than those with low PCAT-1 expression (P<0.001). Both univariate analysis (P=0.000, HR=10.623, 95%CI=5.798-19.464) and multivariate Cox regression analysis (P=0.000, HR=10.996, 95%CI=5.896-20.507) revealed that PCAT-1 could act as a prognostic biomarker for PCa patients.Conclusion: Taken together, overexpression of PCAT-1 is involved in PCa progression and could be a potential prognostic biomarker for PCa patients.
Reductions of Migrant Population Reduces the Number of COVID-19 Epidemic: A Case Study in China
Authors: Han Lizhen , Jia Jinzhu 
Published: November 2020

Abstract
Background: The novel coronavirus disease (COVID-19) broke out worldwide in 2020. The purpose of this paper was to find out the impact of migrant population on the epidemic, aiming to provide data support and suggestions for control measures in various epidemic areas. Methods: Generalized additive model was utilized to model the relationship between migrant population and the cumulative number of confirmed cases of COVID-19. The difference of spatial distribution was analyzed through spatial autocorrelation and hot spot analysis. Results: Generalized additive model demonstrated that the cumulative number of confirmed cases was positively correlated with migration index and population density. The predictive results showed that if no travel restrictions are imposed on the migrant population as usual, the total cumulative number of confirmed cases of COVID-19 would have reached 27 483 (95% CI: 16 074, 48 097; the actual number was 23 177). The increase in one city (Jian) would be 577.23% (95% CI: 322.73%, 972.73%) compared to the real confirmed cases of COVID-19. The average increase in 73 cities was 85.53% (95% CI: 19.53%, 189.81%). Among the migration destinations, the number of cases in cities of Hubei province, Chongqing and Beijing was relatively high, and there were large-scale high-prevalence clusters in eastern Hubei province. Meanwhile, without restrictions on migration, the high prevalence areas in Hubei province and its surrounding areas will be further expanded. Conclusions: The reduced population mobility and population density can greatly slow down the spread of the epidemic. All epidemic areas should suspend the transportation between cities, comprehensively and strictly control the population travel and decrease the population density, so as to reduce the spread of COVID-19.
An Efficient System for Intestinal On-site Butyrate Production Using Novel Microbiome-Derived Esterases
Authors: Jung Dah Hyun , Yong Ji Hyun , Hwang Wontae , Yoon Sang Sun 
Published: November 2020

Abstract
Short-chain fatty acids, especially butyrate, play beneficial roles in sustaining gastrointestinal health. However, due to limitations associated with direct consumption of butyrate, there has been interest in using prodrugs of butyrate. Tributyrin (TB), a triglyceride composed of three butyrate molecules and a glycerol, is a well-studied precursor of butyrate. We screened a metagenome library consisting of 5,760 bacterial artificial chromosome clones, with DNA inserts originating from mouse microbiomes, and identified two clones that efficiently hydrolyse TB into butyrate. Nucleotide sequence analysis indicated that inserts in these two clones are derived from unknown microbes. BLASTp analysis, however, revealed that each insert contains a gene homologous to acetylesterase or esterase genes, from Clostridium spp. and Bacteroides spp., respectively. Predicted structures of these two proteins both contain serine-histidine-aspartate catalytic triad, highly conserved in the family of esterases. Escherichia coli host expressing each of the two candidate genes invariably produced greater amounts of butyrate in the presence of TB. Importantly, administration of TB together with cloned Escherichia coli cells alleviated inflammatory symptoms in a mouse model of acute colitis. Based on these results, we established an efficient on-site and real-time butyrate production system that releases butyrate in a controlled manner inside the intestine.
Effect of Xuezhikang Combined with Rosuvastatin on Lipid Regulation in Patients with Atherosclerotic Cardiovascular Disease: A Real-World Study
Authors: Zhang Liyan , Chen Tong , Zhang Lifang , Xue Zhuyu , Guo Xiaozi , Ding Wei , Zhang Xuejuan 
Published: November 2020

Abstract
Background Lipid control in atherosclerotic cardiovascular disease(ASCVD)patients has always been a focus, and the combination of lipid-regulating drugs has become a major trend. To evaluate the effects of Xuezhikang combined with rosuvastatin on the degree, time, rate and safety of lipid regulation in ASCVD patients.Methods ASCVD patients were randomly divided into Xuezhikang group(group A),rosuvastatin group(group B)and combination group(groupC). Plasma total cholesterol, triglyceride, high density liptein cholesterol and low density lipoprotein cholesterol were measured at various points of observation even the side effects were monitored. Compared carotid intima-media thickness (IMT),Crouse score,plaque stability before and after treatment and calculated the LDL-C success rate. Use appropriate statistical descriptions and analyses based on data characteristics.Results Except HDL-C,TC,TG and LDL-C decreased.There were differences in the regulation of TC and LDL-C among the three groups.The effect of group C on reducing TC was similar to group B and better than group A(PA:B＜0.001,PA：C＜0.001,PB:C=0.175).The effects of reducing LDL-C in group C, group B, and group A decreased successively (PA:B=0.001,PA：C＜0.001,PB:C=0.014).After treatment between groups IMT, Crouse score, plaque stability are improved. The time of reaching the standard of LDL-C in the group C was earlier than that in the group A and group B(PA:B=0.824,PA:C=0.030,PB:C=0.011) and all treatment groups were safe and reliable(p=0.799,p＞0.05).Conclusion Xuezhikang combined with rosuvastatin can significantly reduce plasma TC,TG and LDL-C in ASCVD patients which shorten the time for plasma LDL-C to reach the standard and improve the success rate with both medication safety and the ability to stabilize plaque. It provides a new choice for the combination of lipid-regulating drugs.
Metabolic profiling reveals interleukin-17A monoclonal antibody treatment ameliorate lipids metabolism with the potentiality to reduce cardiovascular risk in psoriasis patients
Authors: Han Cao , Shengmin Su , Qi Yang , Yunchen Le , Lihong Chen , Mengyan Hu , Xiaoyu Guo , Jie Zheng , Xia Li , Yu Yunqiu 
Published: November 2020

Abstract
Background: Psoriasis is a common chronic inflammatory skin disease associated with the overproduction of interleukin-17A. It has been reported that psoriasis patients had an increased risk of cardiovascular diseases. Ixekizumab is a humanized IgG4-κ monoclonal antibody which has shown efficacy in psoriasis patients. Although the pathogenesis in psoriasis and cardiovascular diseases have a remarkable resemblance, the underlying mechanism of two diseases and the possibility of IL-17A monoclonal antibody in the amelioration of cardiovascular comorbidities remains unclear.Methods: Serum samples of two study cohorts including 117 individuals were analyzed using high throughput UHPLC-MS platform. Non-target metabolic profiling analysis was firstly conducted between healthy people, psoriasis patients and Ixekizumab treated patients in study cohort 1. The second study cohort was additionally recruited to validate the correlation of the identified metabolites and cardiovascular diseases. Results: A total of 43 differential metabolites including lysophospholipids, acyl-carnitines and dicarboxylic acids were accurately identified in study cohort 1, which showed impaired lipids metabolism in psoriasis patients. Results of the study cohort 2 largely conform to the previous observations in study cohort 1. Moreover, all identified LPCs levels were higher in psoriasis patients with coronary heart diseases compared with psoriasis patients. It was worth noting that most of these lipidic changes were ameliorated after receiving Ixekizumab treatment.Conclusion: In this non-target metabolic analysis, we found the treatment of IL-17A monoclonal antibody not only can ameliorate the lesions of psoriasis, but also restore the dysregulated lipids metabolism to normal in psoriasis patients. Considering dysregulated lipids metabolism has been regarded as the critical factor in cardiovascular diseases, the recovery of lipidic metabolites in psoriasis patients indicated that IL-17A mAb might have the potential protective effect on cardiovascular comorbidities.
Estimating carbon fixation potential of fallow weeds in rice cropping systems
Authors: Huang Min , Chen Ge , Cao Fangbo , Chen Jiana 
Published: November 2020

Abstract
Fallow weeds are more and more common in Chinese rice cropping systems, but are always excluded in studies of vegetative carbon (C) sinks. This study aimed to evaluate the C fixation potential of fallow weeds in rice cropping systems. A six-region, two-year on-farm investigation and a three-year tillage experiment were conducted to estimate C fixation in fallow weeds in rice cropping systems. The on-farm investigation showed that the average mean C fixation in fallow weeds across six regions and two years reached 112 g m–2. The tillage experiment indicated that no-tillage practices increased C fixation in fallow weeds by 80% on average as compared with conventional tillage. The results of this study not only contribute to an understanding of C fixation potential of fallow weeds in rice cropping systems, but also provide a reference for including fallow weeds in the estimation of vegetative C sink. Further investigations are required to determine the effect of C input from fallow weeds on C balance of rice paddies in order to comprehensively evaluate the role of fallow weeds in C cycling in rice cropping systems.