bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histology-Based Blood Leukocyte Profiling Reveals Parallel Th2 and Th17 Signatures in Seasonal Allergic Rhinitis
Authors: Siddhuraj Premkumar , Nordström Franziska U , Jogdand Prajakta , Elongovan Gayathri , Mori Michiko , Bjermer Leif , Greiff Lennart , Erjefält Jonas S 
Published: February 2020

Abstract
Background : Allergic rhinitis (AR) is one of the most common medical conditions in the westernized world. Recent data suggest AR to be far more immunologically complex than the archetypical allergic Th2-driven eosinophilic inflammation and new methodological approaches are needed to decode this complexity. Methods : This study explores a novel histology-based analysis of circulating leukocytes for detailed profiling of immune cells using routine clinical blood samples. In brief, leukocytes were purified with minimal ex-vivo artefacts, embedded into agarose-paraffin pellets and sectioned for cutting-edge immunohistochemistry-based immune cell profiling. Blood leukocyte mapping was performed in 16 patients with seasonal AR outside and during the birch pollen season. Results : Our methodological feasibility test confirmed that the > 5000 cross sectioned leukocytes typically present in a pellet section had well preserved morphology and cell marker epitopes, allowing for robust quantitative analysis of immune-stained slides. Blood leukocyte samples collected during the allergen season had statistically higher levels of markers for eosinophils, neutrophils, monocytes and CD8 lymphocytes compared to the off-season baseline. No change was observed for CD20 B-lymphocytes, total CD3 T cells and basophils. Subclassification of CD4+ T-helper cells demonstrated a parallel and significant expansion of Th2 and Th17 cells during the pollen season, while Th1 cells remained unchanged. Whereas absolute basophils numbers were unaltered, a significant increase of the basophil markers GATA2 and CPA3 was observed during the pollen season. Conclusions: Apart from representing a positive method feasibility validation, our study provides further evidence of complex and parallel Th2 and Th17 immune signatures in seasonal AR. Our data also forward GATA2 and basophil CPA3 as potential biomarkers for ongoing allergic inflammation. It is thus proposed that the present histology-based approach, with its broad applicability, represents a powerful tool for decoding systemic immune alterations and guide novel biomarker strategies for improved personalized medication.
Adenosine monophosphate-activated protein kinase activator inhibits activation of fibroblast-like synoviocytes but promotes hyaluronan and proteoglycan link protein 1 secretion
Authors: Chen Yong , Fujuan Qiu , Beijia Yu , Fangfang Zuo , Yanan Bi , Ensheng Chen , Xiaofeng Zhao , Yi Yuan , Yanyan Cao , Zhu Shaoyu , Yanan Xuan , Yanjuan Chen , Yongpu Liu , Kaiqin Li , Nandakumar Kutty Selva , Changhong Xiao 
Published: February 2020

Abstract
Objectives: To determine whether any correlation exists between disease activity and AMPK levels in rheumatoid arthritis (RA) patients and investigate the effects of AMPK activator treatment on RA fibroblast-like synoviocytes (RA-FLS).Methods: Serum AMPK-α1, p-AMPK-α1, TNF-α and IL-17 levels between osteoarthritis (OA) and RA patients having different disease activities were compared by ELISA. Differentially expressed genes (DEGs) between RA and OA synovium from NCBI GEO Profiles (accession numbers: GSE1202112, GSE55235, GSE5545713) were identified and the genes intersecting in all the three datasets were selected for enrichment analysis. Immunohistochemical staining was done with synovium obtained from OA and RA patients for p-AMPK-α1. AMPK gene expression in synovium was semi-quantified by RT-qPCR. RNA sequencing of FLS was performed and DEGs were selected for KEGG enrichment analysis. AMPK activator, metformin, treated RA-FLS were tested for proliferation and migration by MTT and scratch test, respectively. Expression of IL-6, AMPK-α1, PKA-α, RAPTOR, mTOR, HAPLN1, RUNX1 and RUNX2 genes were determined by qPCR. Phosphorylated AMPK-α1 and HAPLN1 levels were determined by an automated electrophoresis-western blot analysis method.Results: In RA sera, a positive correlation between p-AMPK-α1 levels and DAS28 (r = 0.270, 95%CI: 0.142-0.492, p < 0.0001) as well as CRP levels (r = 0.259, 95%CI: 0.009-0.478, p < 0.05) was found. Similarly, a positive correlation was observed between AMPK-α1 and TNF-α levels (r = 0.460, 95% CI: 0.241-0.640, p = 0.0002). DEGs between OA and RA synovium from NCBI GEO profiles and our RNA sequencing data suggested activation of metabolic pathways specific to RA-FLS. AMPK-α1 was highly expressed in the synovium of RA but not OA patients. Metformin at higher concentrations inhibited RA-FLS proliferation in a dose dependant manner, however, at lower concentrations it has an opposite effect. On the other hand, AMPK inhibitor, dorsmophin, promoted the proliferation of RA-FLS significantly. Interestingly, both metformin and dorsmophin substantially inhibited the migration of RA-FLS. In FLS, relative expression level of IL-6 mRNA was significantly decreased after metformin treatment, while the expression of AMPK-α1, PKA-α and HAPLN1 genes were significantly increased. Western blot analysis confirmed increased expression of p-AMPK-α1 and HAPLN1 genes in the metformin treated FLS.Conclusions: Inflammatory stress in RA synovium leads to an increase in AMPK levels, possibly as a protective mechanism. AMPK activator but not metformin per se could be a potential therapeutic for RA by promoting HAPLN1 secretion to protect the joints.
A Review on the Recent Flavoring Herbal Medicines of Today
Authors: Pal Rashmi S. , Pal Yogendra , Saraswat Nikita , Wal Pranay 
Published: February 2020

Background:
Herbs are the most trending taste enhancers, carrying multiple benefits. Sprinkling them in minute amounts in pasta, salads, sautéed vegetables, curries, fried rice or adding them in dips and sauces can lead to enhanced flavours. The fresh and dried versions serve the same purpose, especially when the fresh ones are not available.

Objective:
The objective of this article is to explore and review trending flavouring herbs of the present era with reference to the knowledge available from previous texts.

Materials and Methods:
A literature review has been performed on various herbs such as dill, cilantro, parsley, chives, mint, oregano, etc, which can be used as healthy and taste enhancing sprinklers and garnishers for foods.

Results:
There are various herbs present in nature in many forms and patterns, some provide anti-inflammatory benefits, are anti-microbial and most of them are digestive in nature.

Conclusion:
These herbs are very rich in phytoconstituents, having multiple properties like anti-oxidant and carminative effects. They are superior as compared to artificial additives as well.
The Supremacy of Synergism: A Comparison of Anticancer Activity of Rhizome Extract of Bistorta Amplexicaulis and Gallic Acid in Cancer Cell Lines and Primary Cells
Authors: Batool Salma , Asad M. Javaid , Arshad Muhammad , Saleem Rahman Shah Zaib , Ahmad Muhammad Sheeraz 
Published: February 2020

Background:
Bistorta amplexicaulis is a seasonal herb with several folkloric uses. The plant
extract has been shown to possess various activities including antioxidant, anticancer, anti-bacterial,
anti-fungal, cardio-protective, and anti-atherosclerosis activities.

Objective:
The aim of the study was to quantify the activity of the plant extract and relate it to the
activity of the isolated compound, gallic acid.

Methods:
Initially, the plant was extracted, then, the activity of the extract was compared with its
constituent, gallic acid. After this, the cytotoxic potential of the two against human liver cancer cell
line (HepG2), breast cancer cell line (MCF-7) and human umbilical vein endothelial cells (HUVEC)
was evaluated through MTS assay.

Results:
The extract had better activity against HepG2 as compared to gallic acid (IC50 29μg/mL vs
37μg/mL). It also provided a better therapeutic window by having lower toxicity for HUVEC cells
than gallic acid (IC50 63μg/mL vs 47μg/mL), suggesting the use of the extract over the purified gallic
acid for these cells. We also performed the fluorescence study of the rhizome extract in ethanol (REE),
methanol (REM), 80% ethanol (80RE), 80% methanol, (80RM) and acetone (RAC). The highest
intensity of fluorescence was found in REE with excitation at 394 nm and emission at 421nm.

Conclusion:
The comparison of gallic acid with ethanolic rhizome extract of B. amplexicaulis reveals
important insights about utilizing the plant extract over purified gallic acid. The ethanolic extract also has
the potential to be used as an autofluorescent drug during in vitro and in vivo studies.
KAPASITAS SUMBER DAYA MANUSIA SEKSI PENDIDIKAN MADRASAH KANTOR KEMENTERIAN AGAMA KABUPATEN BANDUNG
Authors: Yusuf Ningrum Fauziah , Sintaningrum Sintaningrum , Utami Sawitri Budi 
Published: February 2020

This study aims to look at the human resource capacity of the Ministry of Religion especially in madrasah education. This study wants to explain how the Ministry of Religion in carrying out its duties and responsibilities in the field of madrasah education includes service, guidance, guidance, management of information systems. This study uses a quantitative approach which is analyzed using analysis (Structural Equation Modeling, SEM). Data was collected by informant interviews and questionnaires. As a result, this study shows that currently the human resource capacity of the Ministry of Religion e in the madrasah education section is quite good. This can be seen from the six indicators that researchers use based on the theory of Hall, et al. (2003), which consists of quantity of employees, employee competency standards, employee motivation, reward and punnishment, skill development, and compensation. The results of the study show that in human resource capacity is strongly influenced by the dimensions of employee competency standards and compensation.
THE EFFECT OF OPENING TYPE ON THERMAL CONVENIENCE OF THE OLD AND NEW BUILDING CLASSROOM
Authors: Senoaji Ali Anton , Kusumawanto Arif , Utami Sentagi Sesotya 
Published: February 2020

This study was aimed at analyzing the effect of opening type on the thermal convenience of classrooms in old and new buildings at SMK Negeri 3 Yogyakarta. This study used a qualitative comparative method and the simulation of IES VE 2018. The field air measurement is carried out at 10 measurement points and 5 measurement points in each class, with a height of 1.5 m. Field measurements were carried out in March 2019, at 06.30-16.30 WIB. The parameters used in the study were air temperature, humidity and wind speed. Air temperature and humidity were measured using a Thermo hygrometer. Wind speed was measured using an anemometer. The data collection method is carried out by observation and measurement. Root Mean Squared Error (RMSE) was used to validate the data. The results show the best thermal convenience of the classroom was obtained during the simulation using the type of Windows Awning, with a full aperture area. Simulation results show a comfortable distribution of airflow in the classroom at wind speeds above 0.15-0.28 m/sec, Temperature 25.07-27.10oC.PENGARUH TIPE BUKAAN TERHADAP KENYAMANAN TERMAL RUANG KELAS BANGUNAN LAMA DAN BARU Tujuan dari penelitian yaitu menganalisis pengaruh bukaan terhadap kenyamanan termal ruang kelas pada bangunan lama dan baru, di SMK Negeri 3 Yogyakarta. Penelitian ini menggunakan metode komparatif kualitatif yaitu dan hasil simulasi IES VE 2018. Pengukuran udara luar dilakukan pada 10 titik pengukuran dan sebanyak 5 titik pengukuran disetiap kelasnya, dengan ketinggian 1,5 m. Pengukuran lapangan dilakukan pada bulan Maret tahun 2019, waktu 06.30-16.30 WIB. Parameter yang digunakan dalam penelitian yaitu temperatur udara, kelembaban dan kecepatan angin. Temperatur udara dan kelembaban diukur dengan menggunakan alat thermo hygrometer. Kecepatan angin diukur dengan menggunakan alat anemometer. Metode pengumpulan data dilakukan dengan metode pengamatan dan pengukuran. Validasi data menggunakan Root Mean Squared Error (RMSE). Hasil penelitian menunjukkan kenyamanan termal ruang kelas terbaik diperoleh pada saat simulasi menggunakan tipe bukaan ke atas atau Awning Windows, dengan area bukaan penuh. Hasil simulasi menunjukkan distribusi aliran udara yang nyaman di dalam ruang kelas pada kecepatan angin di atas 0,15-0,28 m/det, Temperatur 25,07 -27,10o C.
THE RELATIONSHIP AMONG PEDICULOSIS CAPITIS, ANEMIA AND LEARNING ACHIEVEMENT IN ELEMENTARY STUDENTS
Authors: Sulistyani Nunung , Khikmah Nur 
Published: February 2020

This study was aimed at determining the relationship between the Pediculus humanus capitis infestation condition, anemia status and learning achievement in primary school-age children. This study was conducted at SD Bangunharjo Sewon Bantul, Yogyakarta. This study is an observational analytic study with cross sectional design in which the variables are measured in one measurement without intervening. The data obtained through pediculosis examination, Hb examination, and measurement of learning outcomes. The data then were analyzed descriptively presented in tabular form, then performed statistical analysis using the chi-square test. The results show that the students suffering from pediculosis, 57.9% also suffering from anemia and 51.0% having poor category achievement. In line with other researchers, one of the factors that influence one’s learning achievement is the memory factor. Someone’s anemia status can affect one’s memory. The prevalence of Pediculus humanus capitis infestation is 38.3%. Chi-square test showed a significant relationship between Pediculus humanus capitis infestation condition, anemia status, and learning achievement.HUBUNGAN PEDIKULOSIS KAPITIS, STATUS ANEMIA DAN PRESTASI BELAJAR PADA SISWA SEKOLAH DASAR Penelitian ini bertujuan untuk mengetahui hubungan kejadian infestasi Pediculus humanus capitis (kutu kepala) dengan status anemia dan prestasi belajar pada anak usia sekolah dasar. Penelitian ini dilakukan di SD wilayah Bangunharjo Sewon Bantul Yogyakarta. Penelitian ini merupakan penelitian analitik observasional dengan desain cross sectional. Variabel diukur dalam satu kali pengukuran dengan tidak melakukan intervensi. Data penelitian diperoleh melalui pemeriksaan pedikulosis, pemeriksaan Hb, dan pengukuran hasil belajar. Data dianalisa secara deskriptif yang disajikan dalam bentuk tabel, kemudian dilakukan analisis statistik menggunakan Uji chi-square. Hasil penelitian ini menunjukan bahwa menderita pedikulosis sebesar 57,9% menderita anemia dan sebesar 51,0% dengan prestasi belajar kategori kurang baik. Salah satu faktor yang mempengaruhi prestasi belajar seseorang yaitu faktor daya ingat. Status anemia seseorang dapat mempengaruhi daya ingat seseorang. Prevalensi infestasi Pediculus humanus capitis sebesar 38,3%. Uji chi-square menunjukkan adanya hubungan yang signifikan antara kejadian infestasi Pediculus humanus capitis dengan status anemia dan prestasi belajar.
The role of marketing intelligence system in achieving customer happiness
Authors: Hemeh Ameen Yousif Obed , Moheden Nishteman Othman 
Published: February 2020

The objective of this study is to measure the nature of the relationship between research variables including the dimensions of marketing intelligent system and customer size. The study investigates whether marketing intelligent system plays the role in obtaining customer happiness in business organizations. The study tried to measure the relationship between marketing intelligence system and its dimension against customer happiness in the given organizations. It also aims to identify the impacts of moral factor of marketing intelligent system and its dimensions on customer happiness. To test given hypotheses, the study used a number of statistical analysis methods such as repetitions, percentages, arithmetic mean, standard deviation, correlation coefficient, and simple linear regression coefficient. The findings indicated that there is a strong positive relationship between marketing intelligence and customer happiness. The study also identified a strong positive correlation between moral factors of marketing intelligence and customer happiness. The study concluded that organizations should take full consideration to the factors that delights customers. Finally, the study recommends that organizations should work through employees to cultivate a culture of customer happiness.
Keywords - Marketing Intelligence, Marketing Intelligence Dimensions, Customer Happiness, Five Star Hotels, Sulaymaniyah City
Internet Use, Depression, and Anxiety in a Healthy Adolescent Population: Prospective Cohort Study
Authors: Thom Robyn Pauline , Bickham David S , Rich Michael 
Published: February 2020

Background
Psychiatric disorders, including conduct disturbances, substance abuse, and affective disorders, emerge in approximately 20% of adolescents. In parallel with the rise in internet use, the prevalence of depression among adolescents has increased. It remains unclear whether and how internet use impacts mental health in adolescents.

Objective
We assess the association between patterns of internet use and two mental health outcomes (depression and anxiety) in a healthy adolescent population.

Methods
A total of 126 adolescents between the ages of 12 and 15 years were recruited. Participants reported their typical computer and internet usage patterns. At baseline and one-year follow-up, they completed the Beck Depression Index for primary care (BDI-PC) and the Beck Anxiety Inventory for Primary Care (BAI-PC). Individual linear regressions were completed to determine the association between markers of internet use at baseline and mental health outcomes at one-year follow-up. All models controlled for age, gender, and ethnicity.

Results
There was an inverse correlation between minutes spent on a favorite website per visit and BAI-PC score. No association was found between internet use and BDI-PC score.

Conclusions
There is no relationship between internet use patterns and depression in adolescents, whereas internet use may mitigate anxiety in adolescents with higher levels of baseline anxiety.
Daftar Isi
Authors: Journal Admin 
Published: February 2020

The Evidence of Goodwill Disclosure at Indonesia’s Listed Company and Goodwill Relationship with Future Earnings Ersa Tri Wahyuni, Hersa Natasha .......................................................................... 415 Otoritas Atasan, Retaliasi dan Locus Of Control Sebagai Faktor-Faktor yang Memengaruhi Perilaku Manipulasi Laporan Realisasi Anggaran Hafiez Sofyani, Yulinda Devi Pramita .................................................................. 427 Faktor-Faktor yang Mempengaruhi Pembiayaan Berbasis Bagi Hasil (Profit and Loss Sharing) Wahyuli Ambarwati Wulandari, Kiswanto ........................................................... 437 Faktor-Faktor Teknis dan Keorganisasian yang Memengaruhi Pengembangan Sistem Pengukuran Kinerja Pemerintah Daerah Warka Syachbrani, Rusdi Akbar ............................................................................. 447 Analisis Wacana Kritis: Menguak Moralitas dan Profesionalisme Bisnis dalam Praktik Akuntansi Pertanggungjawaban Sosial Diska Arliena Hafni, Eko Ganis Sukoharsono, Ali Djamhuri ......................... 465 Reaksi Investor atas Pengungkapan Internet Financial Reporting William Indra S. Mooduto .......................................................................................... 479 Pengaruh Tanggung Jawab Sosial Perusahaan terhadap Kinerja Keuangan dengan Tata Kelola Perusahaan sebagai Pemoderasi Dhesy Eka Putri Shafariani ..................................................................................... 493
PEMAHAMAN TERHADAP TANGGUNGJAWAB, HAK DAN KEWAJIBAN PEGAWAI TERHADAP U.U. NO 5 TAHUN 2014 TENTANG APARATUR SIPIL NEGARA
Authors: Muzayanah Muzayanah 
Published: February 2020

Aparatur Sipil Negara yang kita kenal dengan sebutan ASN merupakan Ujung tombak Pemerintahan terhadap masyarakat yang bertujuan melaksanakan tugas pemerintahan baik yang ada di pusat maupun di daerah seluruh wilayah Negara Republik Indonesia. Sebagai penyelenggara pemerintahan, ASN bertanggungjawab secara menyeluruh untuk memenuhi kebutuhan kehidupan dalam bermasyarakat.Guna mencapai tujuan nasional bangsa Indonesia tersebut diperlukan ASN yang professional, bebas dari intervensi politik, bersih dari praktik korupsi, kolusi dan nepotisme sehingga mampu untuk menyelenggarakan pelayanan publik bagi masyarakat serta mampu untuk melaksanakan peran sebagai perekat persatuan dan kesatuan bangsa berdasarkan Pancasila dan UUD Negara Republik Indonesia Tahun 1945. Penelitian ini merupakan penelitian yuridis normatif dengan spesifikasi penelitan kepustakaan (Library Reasearch) yang bertujuan untuk menjelaskan tentang pemahaman terhadap tanggungjawab, Hak dan Kewajiban ASN pada kewajiban dalam melaksanakan pekerjaan yang menjadi tanggungjawabnya yang sesuai dengan peraturan perundangan yang telah ditentukan. Pemahaman ASN terhadap Tanggungjawab, Hak dan Kewajiban ASN sebagaimana yang telah diatur dalam Undang-undang Nomor 5 Tahun 2014 tentang ASN merupakan upaya dalam mewujudkan ASN yang profesional dalam menjalankan fungsi serta tugasnya untuk menyelenggarakan pemerintahan yang berwibawa dan bertanggungjawab kepada seluruh rakyat Indonesia yang telah digariskan oleh Pendiri negara (The Founding Fathers) Republik Indonesia. Hasil penelitian ini mendiskripsikan bahwa ASN merupakan Pelaksana penyelanggara Pemerintahan Negara Republik Indonesia yang bekerja secara Professional berdasarkansystem merit(cakap dan ahli) di bidang masing-masing. Setiap ASN mampu untuk memahami tanggungjawab, Hak dan Kewajiban bagi dirinya dan berupaya untuk melaksanakannya. ASN memahami tanggungjawab, hak dan kewajiban berdasarkan ketentuan UU no. 5 Tahun 2014 tentang Aparatur Sipil Negara membuktikan bahwa melaui ASN inilah Pemerintah telah berupaya untuk mewujudkan tujuan Nasional bangsa Indonesia melalui ASN yang bekerja dan melakukan kewajiban dalam melaksanakan tugas dan tangungjawab kepada seluruh rakyat. Kata Kunci: Pemahaman ASN, tanggungjawab hak dan kewajiban.
Optimization Of The Government’s Role In Supporting The Socialization Strategy Of Sharia Pawn Products
Authors: Karnawijaya Ning 
Published: February 2020

Sharia pawn products (rahn) are one of the sharia financial services products that are growing over time. However, this development still requires attention in order to achieve financial inclusion and increase market share of sharia pawn products. One of the efforts to realize this goal is the socialization strategy. The success of socialization is not only the responsibility of the Islamic financial services institution, but also the role and support of other stakeholders, especially the government. The government through BI and OJK has a strategic role in helping these efforts. Optimizing the role of the government in supporting the dissemination of sharia mortgage products can be done with strategic steps namely targetting, integrating, understanding, and implementing. Key words : Role of government, Socialization, Sharia pawning