bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a new oral poliovirus vaccine for the eradication end game using codon deoptimization
Authors: Konopka-Anstadt Jennifer L. , Campagnoli Ray , Vincent Annelet , Shaw Jing , Wei Ling , Wynn Nhien T. , Smithee Shane E. , Bujaki Erika , Te Yeh Ming , Laassri Majid , Zagorodnyaya Tatiana , Weiner Amy J. , Chumakov Konstantin , Andino Raul , Macadam Andrew , Kew Olen , Burns Cara C. 
Published: March 2020

AbstractEnormous progress has been made in global efforts to eradicate poliovirus, using live-attenuated Sabin oral poliovirus vaccine (OPV). However, as the incidence of disease due to wild poliovirus has declined, vaccine-derived poliovirus (VDPV) has emerged in areas of low-vaccine coverage. Coordinated global cessation of routine, type 2 Sabin OPV (OPV2) use has not resulted in fewer VDPV outbreaks, and continued OPV use in outbreak-response campaigns has seeded new emergences in low-coverage areas. The limitations of existing vaccines and current eradication challenges warranted development of more genetically stable OPV strains, most urgently for OPV2. Here, we report using codon deoptimization to further attenuate Sabin OPV2 by changing preferred codons across the capsid to non-preferred, synonymous codons. Additional modifications to the 5′ untranslated region stabilized known virulence determinants. Testing of this codon-deoptimized new OPV2 candidate (nOPV2-CD) in cell and animal models demonstrated that nOPV2-CD is highly attenuated, grows sufficiently for vaccine manufacture, is antigenically indistinguishable from Sabin OPV2, induces neutralizing antibodies as effectively as Sabin OPV2, and unlike Sabin OPV2 is genetically stable and maintains an attenuation phenotype. In-human clinical trials of nOPV2-CD are ongoing, with potential for nOPV strains to serve as critical vaccine tools for achieving and maintaining polio eradication.
Mechanisms of multiyear variations of Northern Australia wet-season rainfall
Authors: Sharmila S. , Hendon Harry H. 
Published: March 2020

AbstractNorthern Australia wet season (November–April) rainfall exhibits strong variability on multiyear timescales. In order to reveal the underlying mechanisms of this variability, we investigate observational records for the period 1900–2017. At multiyear timescales, the rainfall varies coherently across north-western Australia (NW) and north-eastern Australia (NE), but the variability in these two regions is largely independent. The variability in the NE appears to be primarily controlled by the remote influence of low frequency variations of El Niño-Southern Oscillation (ENSO). In contrast, multiyear variations in the NW appear to be largely driven locally and stem from a combination of rainfall-wind-evaporation feedback, whereby enhanced land-based rainfall is associated with westerly wind anomalies to the west that enhance local evaporation over the ocean to feed the enhanced land based rainfall, and soil moisture-rainfall feedback. Soil-moisture and associated evapotranspiration over northern Australia appear to act as sources of memory for sustaining multiyear wet and dry conditions in the NW. Our results imply that predictability of multiyear rainfall variations over the NW may derive from the initial soil moisture state and its memory, while predictability in the NE will be limited by the predictability of the low frequency variations of ENSO.
The genetic legacy of extreme exploitation in a polar vertebrate
Authors: Paijmans Anneke J. , Stoffel Martin A. , Bester Marthán N. , Cleary Alison C. , De Bruyn P. J. Nico , Forcada Jaume , Goebel Michael E. , Goldsworthy Simon D. , Guinet Christophe , Lydersen Christian , Kovacs Kit M. , Lowther Andrew , Hoffman Joseph I. 
Published: March 2020

AbstractUnderstanding the effects of human exploitation on the genetic composition of wild populations is important for predicting species persistence and adaptive potential. We therefore investigated the genetic legacy of large-scale commercial harvesting by reconstructing, on a global scale, the recent demographic history of the Antarctic fur seal (Arctocephalus gazella), a species that was hunted to the brink of extinction by 18thand 19thcentury sealers. Molecular genetic data from over 2,000 individuals sampled from all eight major breeding locations across the species’ circumpolar geographic distribution, show that at least four relict populations around Antarctica survived commercial hunting. Coalescent simulations suggest that all of these populations experienced severe bottlenecks down to effective population sizes of around 150–200. Nevertheless, comparably high levels of neutral genetic variability were retained as these declines are unlikely to have been strong enough to deplete allelic richness by more than around 15%. These findings suggest that even dramatic short-term declines need not necessarily result in major losses of diversity, and explain the apparent contradiction between the high genetic diversity of this species and its extreme exploitation history.
Human PZP and common marmoset A2ML1 as pregnancy related proteins
Authors: Kashiwagi Hirofumi , Ishimoto Hitoshi , Izumi Sun-ichiro , Seki Toshiro , Kinami Rihito , Otomo Asako , Takahashi Kazumi , Kametani Fuyuki , Hirayama Noriaki , Sasaki Erika , Shiina Takashi , Sakabe Kou , Mikami Mikio , Kametani Yoshie 
Published: March 2020

AbstractWhile pregnancy-related proteins (PRP) are known to contribute to immunotolerance during pregnancy, their significance to development of invasive placenta is unclear. We compared PRP expression in humans and the common marmoset (Callithrix jacchus), a new-world monkey. Invasive placenta was observed at the maternal-foetal interface of marmoset placenta from green fluorescent protein (GFP)-expressing foetus and wild type mother. The pregnancy zone protein (PZP) and alpha-2 macroglobulin-like 1 (A2ML1) proteins exhibited the most prominent increase in expression during the second trimester in humans and marmoset, respectively. In humans, PZP accumulated at the maternal-foetal interface and A2ML1 accumulated in the amnion. Similarly, A2ML1 mRNA was detected in marmoset placenta. These proteins belong to the A2M family of protease inhibitors, and both PZP and A2ML1 share around 90% homology between human and marmoset and have highly conserved structures. However, the protease-reacting bait regions of the proteins had lower homology (56.8–60.7% in proteins) relative to the rest of the sequence. Notably, the cleavage site of a proinflammatory proline-endopeptidase was preserved in human PZP and marmoset A2ML1. These proteins contain multiple sites that are cleaved by proteases involving proline-endopeptidase. Systemic regulation of these A2M family proteins may be important in animals with invasive placenta.
Quantum Search on Encrypted Data Based on Quantum Homomorphic Encryption
Authors: Zhou Qing , Lu Songfeng , Cui Yongquan , Li Li , Sun Jie 
Published: March 2020

AbstractWe propose a homomorphic search protocol based on quantum homomorphic encryption, in which a client Alice with limited quantum ability can give her encrypted data to a powerful but untrusted quantum server and let the server search for her without decryption. By outsourcing the interactive key-update process to a trusted key center, Alice only needs to prepare and encrypt her original data and to decrypt the ciphered search result in linear time. Besides, we also present a compact and perfectly secure quantum homomorphic evaluation protocol for Clifford circuits, where the decryption key can be calculated by Alice with polynomial overhead with respect to the key length.
First trimester PAPP-A serum levels and long-term metabolic outcome of mothers and their offspring
Authors: Fruscalzo Arrigo , Cividino Adriana , Rossetti Emma , Maurigh Alessia , Londero Ambrogio P. , Driul Lorenza 
Published: March 2020

AbstractLow maternal serum levels of pregnancy associated plasma protein A (PAPP-A) are known to be associated with the development of pregnancy-related complications like small for gestational age infants, intrauterine fetal demise, gestational diabetes and preeclampsia. The study aims to find possible long-term correlations with the development of metabolic and cardiovascular complications in the mothers and their progeny in later life. This is a retrospective cohort study conducted on consecutive unselected women screened for chromosomal anomalies in the first trimester of pregnancy between 2004 and 2010. PAPP-A values as well as clinical data collected at childbirth were considered. A maternal and neonatal follow-up was performed through a telephone interview with the mother during 2015. The body-mass-index and the presence of cardiovascular diseases, dyslipidaemia and diabetes mellitus were evaluated. The analysis included 988 patients. The median time of follow-up was 7 years (IQR 6–9). Lower first trimester maternal blood PAPP-A quartiles were associated with small stature of the offspring (z-score 1st-2nd quartile 0.37 IQR −0.42 and 1.17 vs 3rd-4th quartile 0.67 IQR −0.17 and 1.36, p < 0.05). Furthermore, low first trimester PAPP-A in pregnancy without other gestations following the index one, in Kaplan-Meier analysis was associated to a significant increase of hypoglycemic agents use at 7 and 10 years (respectively 1.12% CI.95 0–2.38% and 5.45% CI.95 0–10.82%) compared to the control group of high first trimester PAPP-A values (0% CI.95 0–0%) (p < 0.05). Low PAPP-A serum levels in the first trimester of pregnancy are associated with short stature in offspring and de-novo development of maternal diabetes mellitus in later life.
Relationship between self-reported and objectively measured manual ability varies during the first year post-stroke
Authors: Hussain Netha , Alt Murphy Margit , Lundgren-Nilsson Åsa , Sunnerhagen Katharina S. 
Published: March 2020

AbstractSelf-reported outcomes provide unique insights about an individual’s perceived manual ability after stroke. This study aimed at determining how the relationship between objective kinematic variables obtained from the target-to-target pointing task and self-reported manual ability varies during the first year in individuals after stroke. Sixty-six individuals from the Stroke Arm Longitudinal study at the University of Gothenburg (SALGOT) cohort were assessed using ABILHAND questionnaire and kinematic analysis at five timepoints between the 10thday and 12thmonth after stroke. Kinematic analysis was performed using a target-to-target pointing task in a virtual environment. Spearman’s correlation was used to determine the extent of correlation between ABILHAND logits and kinematic variables. The correlations varied with time within the first year after stroke. The correlations were low or very low early after stroke and became moderate to high after 6 months for objective measures of movement time and smoothness, but remained low to moderate for mean velocity and low for peak velocity. Due to this discrepancy between self-perceived and objective assessments of arm function, a combination of self-reported and objective assessments of upper limb should be used as outcome measures, especially in the acute and subacute stages after stroke.
Characterization of copulatory courtship song in the Old World sand fly species Phlebotomus argentipes
Authors: Araki Alejandra S. , Brazil Reginaldo P. , Hamilton James G. C. , Vigoder Felipe M. 
Published: March 2020

AbstractAcoustic communication in the form of courtship and mating songs are often involved in reproductive isolation between species of Diptera, such as Drosophila, mosquitoes and sand flies. The patterns of courtship songs in New World sand fly species evolve quickly under sexual selection; and therefore, represent an important trait that can be used as a marker to study the evolution of species complexes and may aid identification of sibling species with a complex. The ability to identify vector species within species complexes is of critical importance for effective and efficient vector control programs. Species-specific song patterns seems to contribute to reproductive isolation in New World sand fly species, suggesting that auditory communication signals may be widespread among these important vectors of leishmaniasis. The main goal of the present study was to characterize the copulatory courtship song of Phlebotomus argentipes, an important vector of visceral leishmaniasis in the Old World. Ph. argentipes males produce acoustic signals during copulation and two types of songs were observed. The one we called primary song is a ‘pulse song’ with similar length and amplitude to the previously observed ‘P1’ pattern recorded in Brazilian populations of Lu. longipalpis s.l. The secondary song has ‘sine song’ characteristics and is quite different from any song produced by New World species. The discovery of this copulation courtship songs in Ph. argentipes supports the possibility that acoustic communication in sandflies might be more widespread than previously thought, including Old World species. Our results highlight the importance of further research on acoustic communication in the Ph. argentipes species complex and other Old World vectors of leishmaniasis.
Assessing the impact of AGS-004, a dendritic cell-based immunotherapy, and vorinostat on persistent HIV-1 Infection
Authors: Gay Cynthia L. , Kuruc Joann D. , Falcinelli Shane D. , Warren Joanna A. , Reifeis Sarah A. , Kirchherr Jennifer L. , James Katherine S. , Dewey Morgan G. , Helms Alyson , Allard Brigitte , Stuelke Erin , Gamble Alicia , Plachco Ana , Gorelick Robert J. , Eron Joseph J. , Hudgens Michael , Garrido Carolina , Goonetilleke Nilu , DeBenedette Mark A. , Tcherepanova Irina Y. , Nicolette Charles A. , Archin Nancie M. , Margolis David M. 
Published: March 2020

AbstractApproaches to deplete persistent HIV infection are needed. We investigated the combined impact of the latency reversing agent vorinostat (VOR) and AGS-004, an autologous dendritic cell immunotherapeutic, on the HIV reservoir. HIV+, stably treated participants in whom resting CD4+ T cell-associated HIV RNA (rca-RNA) increased after VOR exposure ex vivo and in vivo received 4 doses of AGS-004 every 3 weeks, followed by VOR every 72 hours for 30 days, and then the cycle repeated. Change in VOR-responsive host gene expression, HIV-specific T cell responses, low-level HIV viremia, rca-RNA, and the frequency of resting CD4+ T-cell infection (RCI) was measured at baseline and after each cycle. No serious treatment-related adverse events were observed among five participants. As predicted, VOR-responsive host genes responded uniformly to VOR dosing. Following cycles of AGS-004 and VOR, rca-RNA decreased significantly in only two participants, with a significant decrease in SCA observed in one of these participants. However, unlike other cohorts dosed with AGS-004, no uniform increase in HIV-specific immune responses following vaccination was observed. Finally, no reproducible decline of RCI, defined as a decrease of >50%, was observed. AGS-004 and VOR were safe and well-tolerated, but no substantial impact on RCI was measured. In contrast to previous clinical data, AGS-004 did not induce HIV-specific immune responses greater than those measured at baseline. More efficacious antiviral immune interventions, perhaps paired with more effective latency reversal, must be developed to clear persistent HIV infection.
Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality
Authors: Hasan Rajnee , Aktar Nasima , Kabir Shah Md. Tamim , Honi Ummay , Halim Abdul , Islam Rahin , Sarker Muhammad Delwar Hossain , Haque Md. Samiul , Alam Md. Monjurul , Islam Md. Shahidul 
Published: March 2020

AbstractJute fibre is the second most important fibre next to cotton. It is obtained from the bark of plant through microbial retting process. Here we report optimized microbial retting protocol that can lower retting period and produce high fibre quality. A total of 451 bacterial colonies have been isolated from five jute retting water samples in Bangladesh. Higher pectinolytic bacterial isolates were predominant in the later stage of jute retting. Out of these, 168 isolates have been screened by both semi-quantitative and quantitative pectinase, xylanase and cellulase enzyme assay. Among them, 144 isolates have been selected on the basis of extra cellular enzyme activity of these three enzymes. 16 s ribosomal gene sequencing analysis identified 2 phyla- Firmicutis (80.55%) and Proteobacteria (19.45%). To check the synergistic and antagonistic effect 10 selected isolates were tested in 167 different combinations. Three best combinations were identified that lowered retting period from 18–21 days to 10 days producing high quality fibre in both laboratory and field trial. This improved retting technology can be adopted in industrial scale for the production of quality jute fibre in a controlled condition in reduced water quantity without polluting the environment.
Performing the hand laterality judgement task does not necessarily require motor imagery
Authors: Mibu Akira , Kan Shigeyuki , Nishigami Tomohiko , Fujino Yuji , Shibata Masahiko 
Published: March 2020

AbstractWhen people judge the laterality of rotated hand images, that is they perform the laterality judgement task (LJT), they are thought to use motor imagery. However, recent studies have suggested that its completion does not necessarily require the use of motor imagery. In this study, we investigated whether and how many people preferentially use motor imagery to perform the LJT in 37 healthy adults. We assessed the presence of behavioural features associated with motor imagery at the individual level, namely, the linear angle–response time (RT) relationship and the biomechanical constraints effect in the LJT and in the same-different judgement task (SDJT), in which people are not thought to use motor imagery. We found that at most 50% of participants showed both behavioural features in the palmar view condition of the LJT. Moreover, this proportion did not differ from that in the dorsal view condition of the LJT or that in both view conditions of the SDJT. These results demonstrate that a motor imagery–based strategy is not universally and specifically used to perform the LJT. Therefore, previous results of the LJT, in particular, regarding the biomechanical constraints effect, should be reinterpreted in light of our findings.
Intrauterine G-CSF Administration in Recurrent Implantation Failure (RIF): An Rct
Authors: Kalem Ziya , Namli Kalem Muberra , Bakirarar Batuhan , Kent Erkin , Makrigiannakis Antonios , Gurgan Timur 
Published: March 2020

AbstractThis study investigates the effects of intrauterine G-CSF on endometrial thickness, clinical pregnancy rate and live birth rate in a recurrent implantation failure (RIF) group with normal endometrium. This study was designed as a prospective randomized controlled trial with the involvement of 157 RIF group pati; ents. The RIF group was formed on the basis of the RIF criteria: “The failure to achieve a clinical pregnancy after the transfer of at least four good-quality embryos in a minimum of three fresh or frozen cycles to a woman under the age of 40 years. The study sample included 82 patients in the G-CSF group who received G-CSF once a day on hCG. The procedure was performed by administering 30 mIU of Leucostim®(Filgrastim [G-CSF] 30 mIU/mL; DEM Medical, Dong-A; South Korea) through slow infusion into the endometrial cavity using a soft embryo transfer catheter. Normal saline of 1 mL was infused into the endometrial cavity in the same way in 75 patients in the control group. The standard ICSI procedure was used for all patients, and fresh cycle embryos were transferred on the third or fifth day. No statistically significant difference was identified in clinical pregnancy rates, miscarriage rates and live birth rates between the G-CSF group and the control group (p = 0.112, p = 0.171, p = 0.644, respectively), and no difference was observed between the two groups regarding endometrial thickness (p = 0.965). The intervention of administration G-CSF into the uterine cavity in RIF patients with normal endometrium, did not alter the endometrial thickness, clinical pregnancy rates, or live birth rates.
Sfrp1 deficiency makes retinal photoreceptors prone to degeneration
Authors: Cisneros Elsa , di Marco Fabiana , Rueda-Carrasco Javier , Lillo Concepción , Pereyra Guadalupe , Martín-Bermejo María Jesús , Vargas Alba , Sanchez Rocío , Sandonís África , Esteve Pilar , Bovolenta Paola 
Published: March 2020

AbstractMillions of individuals worldwide suffer from impaired vision, a condition with multiple origins that often impinge upon the light sensing cells of the retina, the photoreceptors, affecting their integrity. The molecular components contributing to this integrity are however not yet fully understood. Here we have asked whether Secreted Frizzled Related Protein 1 (SFRP1) may be one of such factors. SFRP1 has a context-dependent function as modulator of Wnt signalling or of the proteolytic activity of A Disintegrin And Metalloproteases (ADAM) 10, a main regulator of neural cell-cell communication. We report that in Sfrp1−/− mice, the outer limiting membrane (OLM) is discontinuous and the photoreceptors disorganized and more prone to light-induced damage. Sfrp1 loss significantly enhances the effect of the Rpe65Leu450Leu genetic variant -present in the mouse genetic background- which confers sensitivity to light-induced stress. These alterations worsen with age, affect visual function and are associated to an increased proteolysis of Protocadherin 21 (PCDH21), localized at the photoreceptor outer segment, and N-cadherin, an OLM component. We thus propose that SFRP1 contributes to photoreceptor fitness with a mechanism that involves the maintenance of OLM integrity. These conclusions are discussed in view of the broader implication of SFRP1 in neurodegeneration and aging.
An ecological analysis of snakes captured by C.J.P. Ionides in eastern Africa in the mid-1900s
Authors: Shine Richard , Spawls Stephen 
Published: March 2020

AbstractHistorical data can clarify ecological attributes of fauna in sites that have subsequently been altered by anthropogenic activities. We used the 1960s notebooks of pioneering East African naturalist C.J.P. Ionides to extract quantitative information on captures of 484 snakes of five species (rhinoceros vipers Bitis nasicornis, black mambas Dendroaspis polylepis, Jameson’s mambas D. jamesoni, water cobras Naja annulata, and eastern forest cobras N. subfulva). High capture rates suggest high abundances of all species. The relative numbers of each species collected changed over the years and differed seasonally, reflecting targeting by Ionides. Sex ratios and age-class distributions differed among species and were affected by factors such as month of collection and time of day. Habitat use was affected by species, sex and body size: for example, arboreality became less common with increasing body size in the rhinoceros viper and black mamba, and males were found in arboreal sites more often than were females. In both D. jamesoni and D. polylepis, adult males and females were recorded together in September-October, suggesting reproductive activity at this time of year. Although fragmentary, the data from Ionides’ notebooks provide a unique glimpse into ecological patterns of snakes within an African landscape half a century ago.