bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cloning, expression and purification of Leptospira LigB antigen in Escherichia coli
Authors: Lan Anh Le Thi , Thi Hang Minh , Thi Thu Hien Nguyen , Thi Ha Giang Pham , Phi Long Trieu , Van Anh Le Thi , Thi Ha Thanh Dao , Thi Thu Thuy Can , Huu Duc Nguyen 
Published: November 2020

Leptospira is one of the most common zoonotic diseases in the tropics and subtropics. Humans are infected by exposure to Leptospira contained water or food sources. Leptospirosis usually breaks out after the flood and causes several consequences for people and economy. Leptospirosis disease, if not rapidly detected and treated promptly, it causes serious consequences such as acute hepatitis-kidneys, meningitis and bleeding, heart and nerve complications, and severe illness can lead to death. Therefore, quick and accurate detection of Leptospira pathogen plays a very important role in Leptospirosis disease treatment. Among antigens of Leptospira, a conserved domain of LigB antigen (Leptospiral immunoglobulin-like protein) was reported that is present in the most of pathogenic serovars of Leptospira, but not in the non-pathogenic Leptospira biflexa, thus this conserved domain was used for production of Leptospirosis detection kits as well as vaccine for Leptospirosis. In order to create a kit for Leptospirosis diagonostic, especially detect anti-Leptospira antibodies in Leptospira infected serum and plasma samples, about 1kb gene fragment encoding for conserved domain of LigB (about 36 kb in molecular weight) was used as the material for producing of LigB protein by DNA recombinant technology. In this study, we present the results for cloning, expressing a conserved domain of LigB antigen in E. coli cells and purifying protein by affinity chromatography collumn. The result indicates that recombinant LigB protein was successfully expressed in E. coli Rosetta 1 and purified by Hitrap chealating collumn. The LigB protein concentration after purification reached 60 mg/L medium with 98% purity. This purified protein will be used as the materials for creating Leptospirosis kit.
Linoleic acid isomerization ability of Lactobacillus spp. isolated from Vietnamese human intestinal origins
Authors: Xuan Thach Tran , Thi Thu Ha , Thi Hien Vu , The Hung Hoang , Thi Hoa Nguyen , Thu Hong Le Thi , Ngoc Anh Luu Dam , Van Huong Bui , Lan Anh La Thi , Van Quyen Dong , Thi Tuyet Nhung Nguyen 
Published: November 2020

Conjugated linoleic acid (CLA) have been shown to exert numerous health benefits, including anti-carcinogenic, anti-atherogenic, anti-diabetic, antiobesity, cholesterol reducing, antioxidant, anti-microbial, immune system modulator and growth-stimulating properties. In human, CLA is produced from Linoleic acid (LA) by gut bacteria. In this study, nineteen Lactobacillus (Lac.) strains isolated from human feces were studied to determine their ability to metabolize LA. The bacteria were grown in the liquid form of anaerobic MRS medium supplemented with 0.5 mg/mL LA. The linoleate isomerase activity in bacteria grown on MRS medium was determined by Gas chromatograpy. The results indicated that 4 out of 19 strains, including strains Lac.02, Lac.05, Lac.14 and Lac.16 are capable of producing about 40-50 μg/mL CLA from LA. Among them, the highest ability to produce CLA from LA is Lac.02 strain. In the production of CLA from LA, enzymes involved in this metabolism in Lactobacillus act as catalysts of hydration/dehydration (CLA-HY), oxidation of hydroxy groups/reduction of oxo groups (CLA-DH), migration of carbon-carbon double bonds (CLA-DC), and saturation of carbon-carbon double bonds (CLA-ER). The cla-dh, cla-dc, cla-hy and cla-er genes that encode enzymes CLA-DH, CLA-DC, and CLA-ER had been found in all Lac.02, Lac.05, Lac.14 and Lac.16 strains. Gas chromatography traces indicated that these strains produced the same compounds, which was subsequently identified as cis-9, trans-11, and trans-10, cis-12 CLA. In the next study, we will optimize the conditions such as substrate concentrations, pH values, temperature and culture time of each strain to obtain the best rerults.
Study on the expression of interleukins in patients with twin-twin transfusion syndrome
Authors: Duy Anh Nguyen , Thi Sim Nguyen , Huyen Thuong Phan , Thi Phuong Thao Tran , Van Tong Hoang , Duy Bac Nguyen , Thi Lan Anh Luong , Thi Thu Canh Than 
Published: November 2020

Twin-twin transfusion syndrome (TTTS) is an unusual and serious condition that occurs in twin pregnancies when identical twins share a placenta but develop discordant amniotic fluid volumes. The TTTS has a high risk of fetal death and birth defects if left untreated. IL-1β, IL-6 and IL-8 have been shown to play an important role in preterm birth in twin pregnancies, which is one of the consequences of TTTS. This study quantified plasma levels of IL-1β, IL-6 and IL-8 in twin pregnant women with (n = 52) and without TTTS (n = 73) by ELISA and analyzed the association of interleukin expression levels with TTTS. The results showed that plasma concentrations of IL-1β and IL-8 were low and not significantly different between the first trimester and the second trimester of pregnancy, between the patients with and without TTTS or between patients with TTTS before and after treatment. IL-6 levels showed no significant difference between first trimester and second trimester of pregnancy or between patients with and without TTTS. Importantly, IL-6 levels were significantly elevated in TTTS patients after surgical treatment compared to those before surgical treatment. In conclusion, IL-6 may be considered as a potential marker for the prognosis of surgical treatment of TTTS.
Study on tuberization of Rehmannia glutinosa (Gaertn.) Libosch.
Authors: Hoai Sam Vu , Thi Xuyen Nguyen , Thi Huong Nguyen , Thi Ngoc Anh Duong , Minh Tuyen Nguyen , Duy Phuong Nguyen , Thuy Hien Phan 
Published: November 2020

Rehmannia glutinosa (Gaertn.) Libosch. is an herbal plant, which belongs to the Srcophulariaceae family, containing the main active ingredients such as catalpol and verbacoside in its root tubers. In traditional medicine, R. glutinosa tubers have been used in the fresh or dried tuber root or the prepared rehamannia root. They not only possess the comprehensive pharmacological actions in the blood system, endocrine system but also used mainly for anti-tumor treament, immune-enhancement, anti-diabetes, treament for concretion in the urinary tract, etc. R. glutinosa is naturally distributed in China, Korea and Japan. This species was introduced into Viet Nam since 1958 and then planted widely in many the Northern plain and midland provinces. In Vietnam, R. glutinosa produces flowers without seeds, therefore the plant has been mainly propagated by slicing tubers. In this work, we investigated the effect of some plant hormones and sucrose contents on the ability to producing microtubers of R. glutinosa. Experiments were establishmented on the 1/4 MS medium supplemented with 50g/L sucrose. Results showed that auxin (IBA, NAA) had no effect on the in vitro tuber formation of R. glutinosa. The efficiency of using the single BAP or PP333 was low. The highest in vitro tuberization rate of R. glutinosa obtained on the medium supplemented with the combination of BAP (1.0 mg/L) and PP333 (0.3 mg/L), reached 83.33% and the number of tubers/plant was 5.58. The optimal sucrose concentration for increasing the diameter and weight of microtubers was 70 g/L. 100% of plants with tuber-roots survived in the nursery and thrived on the field
Atuação do fisioterapeuta nos núcleos de apoio à saúde da família em Teresina, Piauí
Authors: Da Silva Allan Dellon , Nogueira Luciana Tolstenko , Silva Hengrid Graciely Nascimento , Frota Samanta Cris Monteiro 
Published: November 2020

INTRODUÇÃO: A atuação do fisioterapeuta vai muito além do reabilitar, pois este está inserido em todo o processo de produção de cuidado, dessa forma, desempenha um importante papel na atenção básica. OBJETIVO: Analisar a atuação do fisioterapeuta nos Núcleos de Apoio à Saúde da Família em Teresina, Piauí. MATERIAIS E MÉTODOS: Trata-se de um estudo descritivo, com delineamento transversal e abordagem quantitativa. Com aprovação pelo Comitê de Ética em Pesquisa, a coleta de dados se deu por meio da aplicação de um questionário semiestruturado com todos os fisioterapeutas que trabalham no NASF-AB de Teresina-PI. RESULTADOS: Todos os profissionais realizam atendimentos individuais na Unidade Básica de Saúde, e as disfunções mais comumente encontradas estão relacionadas às áreas de Traumato-ortopedia (100%), neurologia (83,33%), reumatologia (50%) e fisioterapia respiratória (33,33%). A maioria dos fisioterapeutas realizam atividades em grupo, ações preventivas e educação em saúde voltada para saúde da criança e adolescentes, saúde da mulher, saúde do homem e saúde do idoso. 100% dos profissionais afirmaram realizar acompanhamentos domiciliares, dentre as atividades mais comuns destacam-se as orientações para pacientes e familiares, prescrição de dispositivos auxiliares de marcha e prescrição de exercícios domiciliares. Além disso, a maioria dos participantes mostrou-se insatisfeito quanto à interdisciplinaridade e interação entre as equipes. CONCLUSÃO: Conclui-se que os profissionais realizam atendimentos tanto individual, como de forma coletiva e os atendimentos ocorrem tanto na unidade básica de saúde como no domicilio. No entanto, ainda é comum relacionarem suas atividades à prática clinica e assistencialista.
Rural community awareness levels, challenges and strategies adopted to combat COVID-19: a cross-sectional e-survey
Authors: Srivastav Adarsh Kumar , Sharma Neha , Goyal Manu , Samuel Asir John 
Published: November 2020

INTRODUCTION: COVID-19 has a widespread effect on rural economies all over the world. It has affecting household incomes, and businesses in every sector of rural economies. Lockdown due to COVID-19 outbreak limiting the outdoor and regular physical activity, and further can leads to increased risk of chronic health conditions. Physical activity and yoga play a vital role in breaking the boredom due to COVID-19 lockdown and thereby, improves the quality of life. OBJECTIVE: To identifying the level of awareness and challenges faced by the people of rural communities due to the COVID-19 outbreak. MATERIALS AND METHODS: A total of 36 participants were recruited by convenient sampling method from five different villages in District, Ambala, Haryana, India. E-survey questionnaires were circulated through WhatsApp messaging mobile-based application. The questionnaire was formed incorporating the three-point Likert scale with three domains, 1) Awareness, 2) Challenges and, 3) Strategies and 12 items. The questionnaire validation was carried out by the experts through Delphi survey method until S-CVI/Ave?> 0.8. The central tendency and dispersion of continuous variable age were expressed in mean with a 95% confidence interval (CI). Categorical data were reported in frequencies as a percentage (%) and sample size (n). The levels of significance were set at 0.05 to minimize Type 1 error. RESULTS: The content validity index of the questionnaire, S-CVI/Ave?=?0.90. By contacting 36 potential participants, a total of 27 survey responses were recorded. Hence, the response rate (RR) was found to be 75%. The mean with 95% CI of age was 30.8 (27.3 to 34.3) years. A total of 94% (n=25) of villagers were aware of COVID-19, 88% (n=24) were facing challenges, and 86% (n=23) of participants are adopting the best strategies which includes, sealing of containment zone, usage of homemade mask made up of clothes, consuming traditional herbal medicine as immunity boosters for prophylaxis and involving in regular physical activity and yoga. CONCLUSION: The rural populations were aware of COVID-19 and having adequate knowledge about COVID-19. They were facing challenges due to COVID-19 and prepared with the best strategies/methods to combat COVID-19.
Avaliação do impacto das abordagens bottom-up e top-down nas atividades da vida diária em pessoas com neglect – revisão sistemática
Authors: Rosa Marlene Neves , Amorim Andreia , Bartolo Marisa , Martins Ana Rita , Oliveira Catarina , Silva Cândida 
Published: November 2020

INTRODUÇÃO: Atualmente não existe um consenso entre quais as caraterísticas das abordagens (bottom-up ou top-down) mais eficazes na reabilitação das Atividades da Vida Diária (AVDs) em pessoas com Síndrome de Neglect (SN). OBJETIVO: Caracterizar as abordagens (bottom-up ou top-down) e o seu impacto nas AVDs em adultos e idosos com SN. MÉTODOS: Esta revisão sistemática foi realizada de acordo com a recomendação PRISMA - Preferred Reporting Items for Systematic Reviews and Meta-Analyses. A pesquisa bibliográfica foi realizada nas bases de dados PubMed, Web of Science, PEDro e Cochrane. Foram considerados estudos experimentais em que pelo menos uma técnica das abordagens bottom-up e top-down fosse utilizada. A ferramenta Joanna Briggs Institute Critical Appraisal Checklist for RCTs foi utilizada para avaliar a qualidade metodológica dos estudos. Foi usada a seguinte combinação de palavras chave: Neglect Syndrome OR Unilateral Syndrome OR Neglect (…) AND Activities of Daily Living OR Daily (…) AND Treatment OR Intervention OR Technique (…). RESULTADOS: Foram incluídos 16 estudos, dos quais 9 incluem técnicas de abordagem bottom-up, 6 incluem técnicas de abordagem top-down e 1 artigo inclui técnicas das duas abordagens. As técnicas da abordagem bottom-up que aumentaram a independência nas AVDs foram Visuomotor Feedback Training, Smooth Pursuit Eye Movement Training e a combinação de Eye Patching com Constraint-induced Therapy. Na abordagem top-down as técnicas com os mesmos resultados foram Visual Scanning, Mental Practice, Continuous Theta Burst Stimulation e Transcranial Direct Current Stimulation. CONCLUSÃO: As abordagens bottom-up e top-down aumentam a independência nas AVDs e cada uma contém técnicas com significativo impacto positivo, como Visuomotor Feedback Training e Continuous Theta Burst Stimulation. O presente trabalho permitiu uma análise crítica à classificação das abordagens em bottom-up e top-down, uma vez que não são sensíveis à distinção dos mecanismos de reabilitação envolvidos.
The relationship between obesity and obstruction risk of lateral inguinal hernia at Negara general hospital in 2019-2020
Authors: Bharata Bagus S. , Triarta Gita B. 
Published: November 2020

Background: A hernia is the protrusion of the abdominal contents from the normal cavity through a defect in the fascia and aponeurotic muscle of the abdominal wall, either congenital or acquired. The hole can arise because the embryonic opening does not close or widen, due to high intra-abdominal pressure caused by chronic coughing, heavy lifting, overweight, obesity, and weakness of the abdominal wall muscles. The incidence of inguinal hernias is higher in patients with body mass index (BMI) who are overweight and obese than those with normal body weight. A study that conducted by Sneiders et al stated that each increase of 1 kg/m2 BMI would increase the risk of hernia complications by 1.03 times (p=0.03).Methods: This study used a cross-sectional analytic study. The samples were determined by consecutive sampling. In this study, the sample used was patients with a diagnosis of inguinal hernia who were divided based on the presence or absence of complications and divided according to BMI, then saw whether there was a relationship between BMI in the obesity category and the incidence of complication inguinal hernia. This study uses data from medical records at the Negara General Hospital from 2019-2020.Results: This study used 79 respondents of hernia patients at the Negara General Hospital who met the inclusion criteria and did not meet the exclusion criteria. There were 77 people (97.5%) male respondents and 2 (2.5%) female respondents with a mean age of 53.05 years (SD±19.7 years). As for the underweight body mass index there were 6 people (7.6%), normal as many as 41 people (51.9%), overweight as many as 4 people (5.1%), and obese as many as 28 people (35.4%). The diagnosis of reducible hernia was 52 people (65.8%), 21 people (26.6%) incarcerated hernia, 5 non-reducible hernias (6.3%) and 1 strangulated hernia (1.3%). Conclusions: The conclusion of this study indicates a significant relationship between BMI and the incidence of complicated hernias. Obese hernia patients were 7.2 times more likely to develop hernia complications than non-obese patients.
Awareness of computer vision syndrome and related factors among information technology professionals
Authors: Jayakumar Gayatri G. , Thampi Bindu , Iyer Mahadevan K. , Sasidharan Rekha R. 
Published: November 2020

Background: Extensive use of computers for both professional and personal purpose has led to an increase in computer related health problems. Aim of this study was to evaluate the awareness regarding symptoms and the related factors in computer vision syndrome (CVS) among a group of information technology (IT) professionals.Methods: This study was conducted among 300 IT employees. After obtaining informed consent, structured questionnaire was given and data collected and analyzed.Results: Out of 300 respondents, 194 (64.7%) were males and 106 (35.3%) females. Mean age was 30.5 years.48.3% had more than eight years of computer usage and 77.3% used computers on an average of 8-12 hours per day. About 201 (67%) of the participants had at least one ocular symptom, most common was dryness (96%). 94.7% reported eyestrain and 86.3% reported redness and itching. Dryness and eyestrain were more in males (p<0.05). Dryness, eyestrain and redness were more in those who used computers for more than eight years duration and for 8-12 hours/day (p<0.05). 253 (84.3%) were aware about this syndrome, the main source being internet. The main relief measure adopted was to take a break in between the work hours. Only 51.3% consulted a doctor for their symptoms. Conclusions: In our study though 84.3% of IT professionals were aware of CVS and 67% had at least one ocular symptom, only 51.3% took professional advice for their problems. There is a definite need for awareness about corrective measures and treatment methods to be adopted for CVS among IT professionals.
An observational study on etiopathogenesis and management in Fistula-in-Ano
Authors: Pancholi Mukesh , Sonvane Shwetal Ravindrabhai 
Published: November 2020

Background: Anal fistulas are abnormal communication between the anal canal and perianal skin or result of anal gland obstruction, with secondary abscess formation and external rupture of the abscess perineum. Fistula in ano rarely heals spontaneously and requires surgical therapy to achieve a cure. Surgical techniques like fistulotomy, fistulectomy, primary closure after excision of tract and staged operations have rendered the postoperative period uneventful, short and steep fall in recurrence rate.Methods: In this retrospective study, 30 patients were selected with diagnoses of fistula in ano admitted in New Civil hospital, Surat, Gujarat during April 2016 to April 2017. Patient underwent definitive treatment. Data related to the objectives of the study were collected from case sheets retrieved from hospital records with permission of competent hospital authority and after approval from ethical committee of institute. Appropriate surgical management were performed. Postoperative findings were noted.Results: Maximum number of patients were in the age group of 30 to 60 years. There were 27 (90%) male patients, 3 (10%) female patients indicating that the disease is more common in male with a ratio of male to female is 9:1. In the study the commonest symptom is discharge in all patients with pruritus in 2 (6.66%) patients and Pain or perineal discomfort in 20 (66.6%) of patients. The commonest sign is presence of external opening in all cases and internal opening in 21 (70%) of patients. Regarding the procedures performed, the majority of the patients 20 patients (66.66%) underwent fistulectomy, while 06 patients (20%) underwent fistulotomy. Seton was tried in 04 patients (13.33%) out of which in 02 patients associated fistulectomy was done.Conclusions: We concluded that the previously burst opened or surgically drained perianal abscess is the main aetiological factor for fistula-in-ano. Operative morbidity is usually low. There is a male preference for the disease and the fistulectomy remains the commonest procedure in our study series.
THE INFUSION SPEECH SKELETON APPLICATION (APP) AS A TEACHING MEDIUM FOR STUDENTS OF OFFICE MANAGEMENT & TECHNOLOGY (OMT): A CONCEPTUAL PAPER
Authors: Ibrahim Shamsinar , Abas Nurliyana , Hashim Norhafiza , Shawalluddin Syahrini 
Published: November 2020

Purpose of the study: This study aimed to utilize the usage of App in Public Speaking course to ease and engage students of Office Management & Technology (OMT) in their learning experience by providing the simplified version of speech draft development.
Methodology: The development of the App divided into two divisions, (a) the App content and, (b) the App design and concept. The content of the App was from the main textbook, simplified to serve the needs of OMT students, whereas the Apps’ design and concept were based on interactive design and waterfall models. Analysis, Design, Development, Implementation, and Evaluation (ADDIE) and Focus Group were also included.
Main Findings: The Infusion Speech Skeleton App is projected to be a vital platform for students to improve their understanding of the course. The App is also expected to improve students’ performance particularly those who have trouble comprehending speech draft development.
Applications of this study: Because the App is mainly developed with OMT students in the loop, most of the settings will be in OMT students’ favors. However, the Apps also can be beneficial to those taking literature, and other communications studies for basic reference.
Novelty/Originality of this study: The App usage in learning was introduced in many courses, however, to our best knowledge this is the first attempt of App development and implementation in the course of public speaking, focusing on the need for non-communication students, OMT students.
GENDER AND PREDICTING INTENTIONS AMONG MALAYSIAN TEACHERS
Authors: Adam Assim Mohamad Ibrani Shahrimin , Janis Salina , Yaccob Yasmin , Mat Jusoh Nurul Hidayu 
Published: November 2020

Purpose of the study: This study examined the predictors of intention in career development, specifically the intention of applying for promotion among Malaysian excellent teacher candidates.
Methodology: The premise of the theoretical analyses in this study was based on the Theory of Planned Behavior. The correlational study design was employed to determine the relationship among the variables that ascertain any prediction towards the intention to apply for promotion. 500 respondents were sampled and consist of male (226) and female (274) teachers who are qualified to apply for promotion. Confirmatory Factor Analyses (CFA) was performed to test and validate the measurement model.
Main Findings: This study had ascertained that gender does moderate relationships among the study's independent variables and the dependent variable. The result also indicates that the theory of planned behavior is applicable in predicting intention to apply for promotion. For teachers in Malaysia, the intention to apply for promotion will increase if the expectation of others towards applying for promotion also positively increase.
Applications of this study: Gender-related specific assessment can be applied within the teachers’ performance appraisal system, particularly for the evaluation on ‘Guru Cemerlang’ or Excellent Teacher Award by the Ministry of Education in Malaysia.
Novelty/Originality of this study: There is a scarcity of recent studies on the intention of Malaysian teachers to apply for promotion. By using the novel approach of Theory of Planned Behavior and the combination of structural equation modeling analyses, gender was found to moderate the relationship between the expectation of others and their intention to apply for promotion.