bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibacterial Effect Of Natural Oils – An Opportunity To Solve The Problem Of Antibiotic Resistance On The Example Of Pseudomonas Spp.
Authors: Leja Katarzyna , Szudera-Kończal Kamila , Myszka Kamila , Czaczyk Katarzyna 
Published: February 2020

Abstract
Presently, the overuse of antibiotics is a great problem all over the world. The reason for this phenomenon is both primary and secondary resistance. Primary resistance is a congenital feature of microbes and does not depend on its contact with a drug. It is chromosomally coded and cannot be transmitted to other species of bacteria. Secondary resistance, on the other hand, develops as a result of contact with the antibiotic substance. Genes located in plasmids are responsible for the formation of this type of resistance. One plasmid often contains resistance genes for several different antibiotics. Plasmids can transfer gene-encoded resistance from one bacterial cell to another by conjugation and transduction. As a result of the overuse of antibiotics in humans and animals, a growing number of infections – such as pneumonia, salmonellosis, tuberculosis, and gonorrhea – are becoming more troublesome to treat. Antibiotic resistance leads also to longer hospital stays, higher medical costs and finally increased mortality. Now people are finally becoming aware of the consequences of the overuse of antibiotics. Thus, interest in natural bacteriostatic materials, such as plant essential oils, has observably grown. A number of scientific studies have confirmed the antimicrobial activity of plant-derived essential oils against pathogenic bacteria, including Pseudomonas aeruginosa. A very important advantage of plant oils is the fact that they are active in low, sub-lethal concentrations, without provoking the acquisition resistance mechanisms in bacteria. The aim of this review was to explain the mechanisms of antibiotic resistance formation on the example of Pseudomonas aeruginosa and to demonstrate that it is worth looking for alternative treatment methods which can lead to limiting the use of antibiotics. Finally, this work tries to explain how the oils work.
1.Introduction. 2. The characteristics of Pseudomonas genus. 2.1. Pseudomonas aeruginosa. 3. The mechanisms of antibiotic resistance in Pseudomonas spp. 3.1. Intrinsic resistance. 3.2. Adaptive resistance. 3.3. Plasmid resistance. 4. The most common resistances of clinical P. aeruginosa strains to antibiotics. 4.1. Resistance to aminoglycosides. 4.2. Resistance to fluoroquinolones. 4.3. Resistance to cephalosporins. 5. Essential oils from plants as a natural alternative for antibiotics. 5.1. Antibacterial activity of plant EOs against Pseudomonas spp. 5.2. How EOs work on the bacteria cell. 6. Summary
Carbapenemase Of Intestinal Rods – The Beginning Of Post-Antibiotic Era?
Authors: Chmielewska Sylwia Joanna , Leszczyńska Katarzyna 
Published: February 2020

Abstract
In recent years in Poland as well as globally at an alarming rate, the number of bacteria producing mechanisms of antibiotic resistance has been increased. The major source of concern is the emergence and dissemination of carbapenem-resistant Enterobacteriaceae (CRE). Carbapenems are considered as last resort drugs for the treatment of multidrug-resistant (MDR) bacterial infections. At the present time the greatest menaces to public health are strains producing KPC (Klebsiella pneumoniae carbapenemases), NDM (New Delhi Metallo-β-lactamase) and OXA-48 (Oxacillinase-48). Carbapenemase-producing Enterobacterales have been resistant to most and sometimes even to all drugs that would be considered for treatment. Therefore, the accurate therapeutic options for the treatment of infections due to CRE strains are limited to the following antibiotics: colistin, tigecycline, fosfomycin, and aminoglycosides. Moreover, combination therapy containing two or more antibiotics has been recommended for the treatment of severe infections caused by carbapenemase-producing Enterobacterales. Due to the rapid spread of carbapenem-resistant strains and the lack of new antibiotic drug development, there is an urgent need to broaden our knowledge regarding antibiotic resistance.
1. Introduction. 2. Carbapenemases. 2.1. Metallo-β-lactamases. 2.2. Class A Carbapenemases. 2.3. Class D Carbapenemases (OXA). 3. Review of antibiotic treatment options of infections due to carbapenem-resistant strains. 3.1. Colistin. 3.2. Fosfomycin. 3.3. Tigecycline. 3.4. Aminoglycosides. 3.5. Carbapenems. 3.6. Mechanism of NDM – likely antibiotic/ chemotherapeutics could be used in the therapy. 3.7. Mechanism of KPC – likely antibiotic/ chemotherapeutics could be used in the therapy. 3.8. Mechanism of OXA-48 – likely antibiotic/ chemotherapeutics could be used in the therapy. 4. Summary
Removal of phenol from aqueous solutions by magnetic oxide graphene nanoparticles modified with ionic liquids using the Taguchi optimization approach
Authors: Gholami-Bonabi Leila , Ziaefar Nasim , Sheikhloie Hossein 
Published: February 2020

Abstract
A magnetic graphene oxide nanocomposite modified by the ionic liquid 1-amino-3-methylimidazole chloride (LI-MGO) was prepared by the chemical coprecipitation method as a phenol adsorbent for the treatment of contaminated aqueous environments. The structure of the prepared nanocomposite was investigated using Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The prepared nanoparticles exhibited a BET (Brunauer–Emmett–Teller) specific surface area of 110.44 m2 g−1 and total pore volume of 0.2839 cm3 g−1. The results revealed that the adsorption process had the highest phenol removal percentage (95.3%) under optimum conditions (pH = 3, nanocomposite concentration = 0.04 g/l at room temperature). Kinetic studies showed a significant fit to the pseudo-second-order kinetic model (R2 &gt; 0.9997) giving an equilibrium rate constant (K2) of 0.000119 gmg−1 min−1 for phenol loaded. The experimental adsorption data were better fitted with the Langmuir isotherm model than with the Freundlich isotherm model. To further investigate the phenol removal optimization process of the modified magnetic nanoparticles, and to determine the effect of each parameter on the adsorption process, the Taguchi optimization approach was used. The adsorption of these synthesized nanocomposites is among the low-cost, high-efficiency processes that can be used for the reduction/elimination of environmental pollutants, especially in aqueous environments.
Energy saving for air supply in a real WWTP: application of a fuzzy logic controller
Authors: Bertanza G. , Menoni L. , Baroni P. 
Published: February 2020

Abstract
An unconventional cascade control system, for the regulation of air supply in activated sludge wastewater treatment plants (WWTPs), was tested. The dissolved oxygen (DO) set point in the aeration tank was dynamically calculated based on effluent ammonia concentration, following a fuzzy logic based approach. First, simulations were conducted, according to the BSM2 protocol, for a general comparison with more conventional control strategies. It turned out that the effluent quality could be improved by 7–8%, based on the EQI parameter. Moreover, the aeration energy requirement could be reduced up to 13%. Subsequently, the system was installed in a full-scale WWTP. While stably complying with the ammonia effluent standard (10 mg/L), excess air supply was prevented, and a reduction of the specific power consumption (kWh/kgCODremoved) of 40–50% was recorded with respect to the previously installed PID controller (fixed DO set point).
Identification of Salmonella Typhimurium Peptidyl-prolyl cis-trans Isomerase B (PPIase B) and Assessment of their Role in the Protein Folding
Authors: Kumawat Manoj , Karuna Irungbam , Ahlawat Neeraj , Ahlawat Sushma 
Published: February 2020

Background:
Peptidyl-prolyl cis-trans isomerase (PPIases) enzyme plays a vital role in
protein folding. It catalyses the cis-trans isomerisation of peptide bonds, an essential step for newly
synthesized protein to acquire its correct functional conformation in both prokaryotes and
eukaryotes.

Objective:
The present study showed the biochemical and molecular characterisation of
cyclophilins (PpiB), a type of peptidyl-prolyl isomerases proteins from the pathogenic bacteria
Salmonella Typhimurium.

Methods:
Salmonella Typhimurium is one of the leading serovars responsible for human and
animal salmonellosis globally, with the majority of human cases originating through the food chain.
Here successful expression and purification of PpiB protein have been demonstrated and LC-MS
based analyses showed high protein score and similarity with other PPi protein. Further the
enzymatic activity of the purified recombinant PpiB was determined using Succinyl-Ala-Phe-Pro-
Phe-p nitroanilide as substrate and enzyme-catalysed reaction.

Result:
Km and Vmax were calculated and found to be Vm = 1.023 ± .06400 min/μg, Km = 0.6219
± 0.1701 μM, respectively. We have reported for the first time the presence of Salmonella PPIase-B
(PpiB) protein isoforms in salmonella genome having PPi activity.

Conclusion:
Taken together, our data clearly showed that Salmonella Cyclophilin B (PpiB) protein
is active and involved in diverse biological processes and highly similar to the different domain of
Cyclophilin proteins.
Curcumin Attenuates Cerebral Ischemia-reperfusion Injury Through Regulating Mitophagy and Preserving Mitochondrial Function
Authors: Wang Weiwei , Xu Jiaping 
Published: February 2020

Background:
Curcumin, the complex extracted from the traditional edible herb, has a
wide range of pharmacological effects. A great deal of studies has demonstrated that curcumin
could protect against cerebral ischemia-reperfusion (I/R) injury. In the present study, we aimed to
test the hypothesis that curcumin reduces brain damage via regulating mitophagy and preserving
mitochondrial function. To clarify the potential effect and mechanism of curcumin on cerebral I/R,
we utilize MCAO followed by reperfusion rats and OGD/R neurons as cerebral I/R in vivo and in
vitro, respectively.

Methods:
We determined the cellular ROS levels and mitochondrial function, including mitochondrial
membrane potential (MMP), ATP levels, state 3 respiration and state 4 respiration. We also
detected the levels of mitophagy by immunofluorescent staining and western blotting.

Results:
Results found that curcumin decreased neurological deficit scores, infarct volume and
morphological changes of neurons in rats after brain I/R injury. Curcumin also reduced the levels
of ROS while increased MMP, ATP levels and state 3 respiration to prevent the impairment of mitochondrial
function from cerebral I/R. Furthermore, curcumin enhanced the co-localization of
LC3B and mitochondrial marker VDAC1, the ratio of LC3-II to LC3-I, improving cerebral I/Rinduced
mitophagy.

Conclusion:
In conclusion, our results suggest that curcumin protects against cerebral I/R injury
by improving mitophagy and preserving mitochondrial function.
Analysis of Oxaliplatin Resistance in Colorectal Cancer Cells by Combined Proteomics and Phosphoproteomic
Authors: Yang Rui , Feng Kunli , Cao Yanhong , Wang Hao , Wang Baolong 
Published: February 2020

Background:
Oxaliplatin(Oxa) is a major chemotherapy drug for colorectal cancer.
However, drug resistance is a major cause of treatment failure for late-stage colorectal cancer.
Therefore, it is necessary to explore the mechanism of resistance to oxaliplatin in HCT116 colorectal
cancer cells.

Objective:
Therefore, this study explored the mechanisms of HCT116 cells resistance to oxaliplatin
by combining the results of proteomic and phosphoproteomic analyses.

Methods:
In this study, firstly,we constructed oxaliplatin-resistant HCT116 cells called HCT116/
Oxa. Then, we conducted a quantitative study of phosphoproteomics in HCT116 and HCT116/
Oxa cells via TMT labeling, bio-material-based PTM enrichment, HPLC fractionation, and
LC-MS/MS analyses. At the same time, we applied TMT/iTRAQ labeling, HPLC fractionation,
and LC-MS/MS to conduct proteomic and phosphoproteomic analyses of the cell lines. Finally, we
analyzed the results from Gene Ontology (GO), protein domain, and Kyoto Encyclopedia of Genes
and Genomes (KEGG) pathways using the 1.5 change rate as a meaningful change threshold.

Results:
Our analysis confirmed the previously described mechanisms of colon cancer resistance
and revealed the important role of phosphorylation in drug resistance.

Conclusion:
Collectively, this study provides a new direction for the study of oxaliplatin resistance
in colorectal cancer.
Archeologie in musea: een passend verleden voor de Sami?
Authors: van den Berg Mathilde 
Published: February 2020

A Sami past.
The Sami are still struggling for general acceptance of their identity and cultural expression. They are the indigenous population of Fennoscandia, and although now mostly associated with reindeer herding, historically their subsistence was based mostly on fishing, hunting, agriculture, animal husbandry, and only small-scale reindeer herding. In this paper it is probed how contemporary majority and Sami museums convey information about the Sami and their past, with a special focus on the role of archaeology. This is important because museums have an active role in the creation of knowledge and identity. There are several discrepancies between what archaeology is and can present, and how the Sami see their culture and past. Archaeology works with linear time, and focuses on material culture that is, landscape-wise, taken out of context. By contrast, Sami culture emphasizes the importance of material culture within the landscape, feels affiliated with circular time and nature, and does not accept all material culture that archaeology classifies as Sami. In the case of the Sami, archaeology is a less-than-ideal way to communicate about their past and culture.
Q130: surveys op het terrein van een hellenistische en Romeinse boerderij in zuid-Italië
Authors: van Leusen Martijn , Oome Neeltje 
Published: February 2020

Q130: surveys over the site of a Hellenistic-Roman farm in southern Italy.
This article reports on the results of survey experiments conducted by a GIA team in 2010 over the site of a Hellenistic-Roman farm, Q130, located in the foothills enclosing the Sibaritide coastal plain in southern Italy. The experiments show that a ‘quick scan’ surface survey method achieves good results in locating the main structures, matching the results of a gradiometer survey conducted simultaneously, and that further useful chronological and functional detail was obtained by ‘total’ survey and in-field classification of the finds. The socio-economic context of this farm site must obviously be sought in the nearby Hellenistic colony of Thourioi and its Roman successor Copiae, but further questions about the character and chronology of the site can only be answered by excavation.
PEMBIASAAN MENGGOSOK GIGI UNTUK MENJAGA KESEHATAN GIGI DAN MULUT
Authors: Arumsari Fitria 
Published: February 2020

Berawal dari banyaknya anak yang tidak gosok gigi saat pergi ke sekolah yang dikarenakan kurangnya kesadaran orang tua tentang pentingnya menjaga kesehatan gigi dan mulut anak dan juga kesibukan orang tua dalam bekerja, maka guru membuat pembiasaan yaitu menggosok gigi di sekolah. Gosok gigi dilakukan setiap hari sebelum proses belajar mengajar berlangsung dan diikuti oleh seluruh siswa TK dari kelompok A dan B. Dalam pelaksanaannya guru menggunakan metode demonstrasi. Guru memberi contoh cara menggosok gigi yang benar dan anak mengikuti. Hasil dari kegiatan ini diharapkan anak memahami pentingnya menjaga kesehatan gigi dan mulut. Selain itu anak menjadi rajin menggosok gigi di sekolah maupun di rumah, sehingga anak dapat memiliki gigi yang sehat.Kata kunci: Pembiasaan, gosok gigi, anak TK
MENULIS ITU MENYENANGKAN
Authors: Syamsidah Syamsidah 
Published: February 2020

Menulis merupakan aktifitas menuangkan ide berupa pengalaman nyata atau cerita rakyat yang disebut dengan tulisan non fiktif dan cerita khayalan yang disebut dengan tulisan fiktif. Menulis membutuhkan beberapa persiapan antara lain motivasi, dukungan sahabat atau bisa bergabung dalam komunitas penulis serta alat tulis seperti komputer. Beberapa aspek tersebut merupakan komponen dalam persiapan internal, persiapan eksternal dan persiapan peralatan. Cara menulis perlu diketahui penulis pemula agar menulis menjadi aktifitas yang menyenangkan. Langkah yang perlu dilakukan yaitu memulai menentukan jenis tulisan fiksi atau nonfiksi kemudian membuat kerangka atau peta konsep agar hasil tulisan bisa berkualitas dan dapat dipublikasikan. Hasil tulisan yang dapat dipublikasikan dalam bentuk buku maupun jurnal dapat memberi keuntungan berupa royalti dan menambah angka kredit untuk kenaikan pangkat. Kata Kunci: Menulis, Publikasi
KEMAMPUAN MENULIS AWAL MELALUI PERMAINAN KREATIVITAS
Authors: Halimah Nur 
Published: February 2020

Perkembangan menulis awal anak tidak akan berkembang begitu saja seiring dengan kematangan usia, tetapi adanya dorongan dari luar maka perkembangan tersebut dapat terjadi. Kegiatan bermain yang dilakukan bertujuan untuk mengembangkan menulis permulaan pada anak usia 5-6 tahun melalui kegiatan bermain kreatif pada anak SD tingkat rendah di SD Negeri 1 Pining, Kecamatan Pining Kabupaten Gayo Lues. Kemampuan menulis awalpada kelas rendah masih sangat kurang itu disebabkan keterbatasan guru dalam memberikan kegiatan yang kurang menarik bagi anak. Permainan kreatif sebagai salah satu alternatif yang bisa dilakukan guru agar dapat mengembangkan menulis awal dengan kegiatan yang menimbulkan rasa senang. Kata Kunci : perkembangan menulis awal, bermain kreatif.
BUDIDAYA JAMUR TIRAM PUTIH (Pleurotus ostreatus) SEBAGAI PERCONTOHAN DAN UNIT USAHA BUDIDAYA JAMUR (UUBJ) DI UNIVERSITAS CENDERAWASIH
Authors: Agustini Verena , Sufaati Supeni , Bharanti Bonifasia Elita , Runtuboi Dirk Yanes 
Published: February 2020

Usaha jamur tiram putih (Pleurotus ostreatus) di Papua merupakan “bisnis baru” yang sangat menjanjikan. Unit Usaha Budidaya Jamur (UUBJ) yang merupakan implementasi program PPUPIK Jurusan Biologi FMIPA Universitas Cenderawasih (Uncen) telah merintis budidaya jamur tiram sejak tahun 2016. Jamur segar yang dihasilkan 15 kg per minggu dengan harga Rp. 70.000,-/kg. Selain sebagai profit, UUBJ juga menjadi sarana pembelajaran bisnis (wirausaha) bagi mahasiswa, alumni dan kelompok masyarakat. Solusi atau teknologi untuk menumbuhkan dan membentuk jiwa kewirausahaan adalah Entrepreunership Capacity Building (ECB) dan Participatory Action Learning System (PALS). Unit ini telah menghasilkan 5 orang entrepreuner baru di bidang usaha jamur tiram putih yang berasal dari mahasiswa dan alumni.
Penyuluhan Alat Deteksi Kesadahan Dalam Air Berbasis Light Dependent Resistor Di Kelurahan Giritontro, Kecamatan Giritontro, Kabupaten Wonogiri
Authors: Sumarna Sumarna , Agustikasa Dyah Kurniawati , Purwanto Agus , Kadarisman Nur , Nugraheni Anggiyani Ratnaningtyas Eka , Dina Dina 
Published: February 2020

Beberapa daerah di Wonogiri memiliki tanah yang mengandung kapur, sementara itu sebagian masyarakat mengonsumsi air yang berasal dari dalam tanah. Pada kegiatan pengabdian kepada masyarakat tahun 2016 ditemukan permasalahan dalam masyarakat di kelurahan Wonogiri yaitu sulitnya mendeteksi adanya kapur dalam air yang mereka konsumsi. Sebagai akibatnya banyak masyarakat yang menderita penyakit dalam yang diakibatkan pengendapan kapur di dalam tubuh mereka seperti penyakit ginjal. Oleh karenanya, tim PPM Fakultas MIPA Universitas Negeri Yogyakarta membangun sistem pendeteksi adanya kapur dalam cairan berbasis Light Emitting Diode (LED) dan Light Dependent Resistor (LDR). LED digunakan untuk menyinari cairan dengan kadar kapur yang berbeda-beda, kemudian LDR akan mendeteksi cahaya LED yang melewati cairan tersebut. Besar kecilnya nilai luaran LDR akan bergantung kadar kapur dalam cairan. Masyarakat sangat antusias dan mulai menggunakan alat ini untuk mendeteksi adanya kapur di air yang mereka konsumsi. Kata kunci: Kesadahan, LDR, LED Workshop On Detector of Water Hardness Based On Light Dependet Resistor In Giritontro Village, Giritontro District, Wonogiri RegencyAbstract Some areas in Wonogiri contain hardness in its soil, while some people consume water that come from the soil. In community service activity in 2016, we found problems in the community in Giritontro, Wonogiri that is the difficulties to detect the hardness in the water that people consume. As a result, many people who suffer from internal diseases caused by the deposition of lime in their bodies such as kidney disease. Therefore, the PPM team of the Faculty of Mathematics and Natural Sciences of Yogyakarta State University built a hardness detection system in liquid based Light Emitting Diode (LED) and Light Dependent Resistor (LDR). LEDs are used to irradiate fluids with different lime levels, then LDR will detect the LED light passing through the liquid. The size of the LDR output value will depend on the lime content in the liquid. People are very enthusiastic and start using this tool to detect the presence of limestone in the water they consume. Keywords: Hardness, LDR, LED
Peristiwa Pemberantasan PGRS-Paraku di Kalimantan Barat Tahun 1967
Authors: * Yulita Dewi Purmintasari 
Published: February 2020

Penelitian ini bertujuan:mendapatkan informasi yang objektif mengenaiperistiwa pemberantasan PGRS/Paraku di Kalimantan Barat, analisis teori hegemoni Gramsci terhadap peristiwa pemberantasan PGRS/Paraku, pembelajaran sejarah lokal melalui materi peristiwa pemberantasan PGRS/Paraku. Penelitian ini menggunakan metode deskriptif dengan bentuk studi kasus terpancang. Sumber data adalah narasumber dari dosen dan mahasiswa. Teknik pengumpulan data berupa observasi langsung dengan panduan observasi dan komunikasi langsung dengan panduan wawancara.Hasil penelitian menunjukkan: PGRS/Paraku dibentuk ketika terjadi konfrontasi Indonesia-Malaysia. Namun ketika Orde Baru, PGRS/Paraku yang menjadi garda terdepan dalam konfrontasi diperangi. Dalam penumpasan PGRS/Paraku sebagai bentuk kecil hegemoni di Kalimantan Barat, tindakan kekerasan yang bersifat memaksa dilakukan oleh militer, dalam hal ini angkatan darat. Masyarakat Dayak di perbatasan menghimpun kekuatan bersama ABRI melawan komunis yang telah memecah belah mereka dan menumpas PGRS/Paraku.Melalui kajian hegemoni Gramsci, kajian ini dapat menjadi materi pembelajaran sejarah lokal yang perlu dikembangkan untuk menambah pengetahuan serta meningkatkan nasionalisme dan patriotisme generasi mendatang. Kata Kunci : PGRS/Paraku, Hegemoni Gramsci
PENGARUH PENGGUNAAN WAKTU MENONTON TELEVISI, INTERNET, DAN HANDPHONE TERHADAP DISIPLIN BELAJAR SISWA DI LOMBOK
Authors: Tri Hartiti Aliahardi, 
Published: February 2020

Penelitian ini bertujuan mengungkapkan pengaruh penggunaan waktu menonton televisi, Internet, dan handphone terhadap disiplin belajar siswa di Desa Kopang, Lombok Tengah. Sumbangan efektif (SE) penggunaan waktu menonton televisi sebesar 43,1%, mengakses Internet sebesar 13,8%,dan mengakses handphone sebesar 0,1% terhadap disiplin belajar siswa. Besarnya sumbangan efektif penggunaan waktu menonton televisi, mengakses Internet, mengakses handphone terhadap disiplin belajar siswa adalah 57%%, sedangkan sisanya 43% dipengaruhi oleh variabel lain yang tidak dibahas dalam penelitian ini. Dengan demikian pengaruh penggunaan waktu menonton televisi memberikan sumbangan positif paling besar terhadap disiplin belajar siswa. Kata kunci: menonton televisi, mengakses internet, mengakses handphone dan disiplin belajar