bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and safety of guselkumab for the treatment of patients with moderate-to-severe plaque psoriasis: A metaanalysis of randomized clinical trials
Authors: Tao Xing-Bao , Huang Yin-Qiu , Zhou Yi-Hong , Zhang Lv-Lang , Chen Yao-Kai 
Published: June 2020

Purpose: To conduct a systematic analysis on data from randomized controlled trials (RCTs) on different doses of guselkumab, and provide high-quality evidence for its use in the treatment of patients with moderate-to-severe plaque psoriasis (PsO).
Methods: Related studies were searched using online search engines including MEDLINE, PubMed, and central registry of Cochrane controlled trials from January 2001 to October 2017. Only randomized, placebo-controlled, double-blind clinical trials involving guselkumab- and placebo-treated PsO subjects were included.
Results: Five eligible double-blind, randomized, and placebo-controlled trials involving patients with moderate-to-severe PsO subjects treated with guselkumab were included. Compared with the placebo groups, the proportion of patients with improvements in Psoriasis Area and Severity Index (PASI) 75 (RR= 12.14; 95% CI= 9.11-16.16; p < 0.001); PASI 90 (RR= 23.26; 95% CI =14.57-37.13; p < 0.001), and PASI 100 (RR = 37.66; 95% CI = 15.81-89.69; p < 0.001) were significantly higher than those in guselkumab-treated groups. Furthermore, the guselkumab-treated groups showed significant decreases in Physician’s Global Assessment (PGA) score (RR = 10.46; 95% CI = 7.96-13.83; p < 0.001) and the Dermatology Life Quality Index (DLQI) score (SMD = -1.3; 95% CL = -1.4 to -1.19; p < 0.001), when compared with the placebo groups. However, there were no significant differences in adverse events (AEs) (RR = 1.01; 95% CL = 0.93-1.11; p > 0.05); severe adverse events (SAEs) (RR = 1.32; 95% CI =0.69-2.54; p > 0.05) and study discontinuations (RR = 0.79; 95% CI = 0.42-1.48; p > 0.05) between the two groups.
Conclusion: This meta-analysis summarizes available evidence for the use of guselkumab in psoriasis. The results suggest that guselkumab is superior to placebo in moderate-to-severe psoriasis, and is welltolerated, effective, and safe in improving the severity of disease and quality of life.
Keywords: Guselkumab, Effectiveness, Safety, Plaque psoriasis, Meta-analysis, Quality of life
Proteomics analysis of differentially-expressed proteins in uterus of primary dysmenorrhea mice following administration of nuangong zhitong
Authors: Xie Yazhen , Qian Jianqiang , Lu Qibin 
Published: June 2020

Purpose: To use label-free proteomic method to investigate the mechanism of action of nuanggong zhitong decoction (NZD) on primary dysmenorrhea (PD).
Methods: A mouse model of PD was established through oxytocin administration. The mice were divided into control group (normal mice), model group (PD mice administered normal saline), and treatment group (mice given NZD). The serum levels of PGE2 and PGF2α in the mice were measured by ELISA. The differentially expressed proteins (DEPs) among the three groups were revealed by identifying the proteins that were up-regulated (or down-regulated) in model group and down-regulated (or up-regulated) in the treatment group. The DEPs in the three groups were identified using Nano- HPLC-MS/MS, and their functions were investigated using bioinformatics analyses. The accuracy of proteomics was verified with western blot analysis.
Results: Thirty-eight up-regulated and 66 down-regulated DEPs were identified. Bioinformatics analysis revealed that the DEPs were related to immune response, signal conduction, protein binding, and metabolism. STRING analysis indicated a total of 53 DEPs have direct or indirect functional links. Western blot results revealed that levels of Stat1, Rock1, vinculin and vaveolin-1 were consistent with the results of proteomic analysis.
Conclusion: These findings provide further insights into the mechanism underlying the protective effects of NZD.
Keywords: Primary dysmenorrhea, Uterus, Nuangong zhitong decoction, Vinculin, Caveolin, Differentially expressed proteins (DEPs), Bioinformatics
Effect of dexmedetomidine post-treatment on oxidative stress and apoptosis induced by myocardial ischemiareperfusion injury in rats
Authors: Geng Zhihai , Zhu Xuelian , Han Xi , Xin Xianfeng 
Published: June 2020

Purpose: To study the effect of dexmedetomidine on myocardial ischemia-reperfusion injury (MI/RI)- induced imbalance on oxidant-prooxidant status and apoptotic changes in rats.
Methods: Ninety (90) male Wistar rats were randomly divided into three groups – sham, model and post-treatment. In model rats, the anterior descending branch of the left coronary artery was ligated for 25 min, prior to their being subjected to reperfusion for 2 h. Rats in the post-treatment group were subjected to ligation at the anterior descending branch of the left coronary artery for 25 min, but they were intravenously injected with dexmedetomidine at a dose of 10 μg/kg prior to reperfusion. There was no ligation in the sham group. Malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were assayed. Lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) levels were also evaluated. Apoptosis was measured with TdT-mediated dUTP nick end labeling (TUNEL) assay.
Results: Compared with the sham group, MDA level in the model group was significantly rose, while SOD and GSH-Px activities were markedly decreased (p < 0.05). Moreover, there were higher LDH and CK-MB activities in model rats than in the sham rats, but they were significantly lower in the posttreatment group than in the model group (p < 0.05). Apoptosis was higher in model rats than in sham operation rats, but was markedly decreased in post-treatment rats than in model rats (p < 0.05).
Conclusion: Post-treatment with dexmedetomidine exerts myocardial protective effect via significant reduction in oxidative stress-induced myocardial injury and apoptosis.
Keywords: Dexmedetomidine, Myocardial ischemia-reperfusion injury, Antioxidant status, Programmed cell death
Hepatoprotective effect of desi and kabuli cultivars of <i>Cicer arietinum</i> L (chick peas) against carbon tetrachloride-induced toxicity in rats
Authors: Majeed Mudasir , Hussain Abdullah Ijaz , Anwar Haseeb , Irfan Shahzad , Chatha Shahzad Ali Shahid , Ali Qasim , Mukhtar Imran , Hafeez Zahra 
Published: June 2020

Purpose: To determine the hepatoprotective potential of ethanol extracts of desi and kabuli cultivars of Cicer arietinum L. (chick peas).
Methods: Hepatotoxicity was induced in rats using oral administration of carbon tetrachloride (CCl4). The rats were then orally administered different doses of the ethanol extracts of desi and kabuli cultivars of Cicer arietinum L. for 21 days. Oxidative stress parameters and hepatoprotective profiles were determined in serum samples using standard procedures. The effect of the treatments on liver histology was also determined.
Results: Administration of extracts of desi and kabuli cultivars of Cicer arietinum L. to CCl4 treated rats at a dose of 300 mg/kg resulted in a significant (p ≤ 0.05) decrease in oxidative stress parameters, whereas catalase activity significantly increased (p ≤ 0.05); on the other hand, ALT and AST levels were decreased significantly (p ≤ 0.05), when compared to the control group.
Conclusion: High doses of Cicer arietinum L (desi and kabuli cultivars) seem to have hepatoprotective and antioxidant effects on CCl4-induced toxicity in rats. This finding underscores the therapeutic importance of Cicer arietinum L. as a plant with hepatoprotective properties.
Keywords: Cicer arietinum, Phenolics, Hepatotoxicity, Chick peas, Catalase
Extraction optimization of <i>Eucommia ulmoides</i> Oliver and its effect on bone quality in OVX rats
Authors: Zhao ZhengNan , Yang XiangLong , Li Maoxun , Yu Fang , Liang HaiDong 
Published: June 2020

Purpose: To maximize the yield of extract from Eucommia ulmoides Oliver and its effect on bone quality.
Methods: Different extraction indices were optimized with response surface methodology (RSM) for maximization of extract yield from Eucommia ulmoides Oliver. Box–Behnken design (BBD) was used to identify the effects of temperature, time, and liquid to solid ratio on extract yield from Eucommia ulmoides Oliver. After 4-week acclimatization, thiry-two rats were randomly assigned to 4 groups (n = 8): group 1 (sham) given vehicle only; group 2 (OVX rats given Eucommia ulmoides Oliver extract at a dose of 4 g/kg; group 3 (OVX + vehicle); group 4 (OVX + EUOE), i.e., OVX rats given Eucommia ulmoides Oliver extract (4 g/kg). Sham rats had intact ovaries. After surgery, the rats received gentamicin intramuscularly for 3 successive days. Two months after surgery, blood and trabecular bones was taken for analysis.
Results: Temperature and liquid-to-solid ratio had marked impact on extract yield from Eucommia ulmoides Oliver, with the best conditions being temperature of 88 °C, time of 137 min, and liquid to solid ratio 16:1. Using these optimized conditions, the maximum yield of extract obtained experimentally (2.53%) was very close to the predicted value of 2.49 %. There was a good fit between the mathematical model evolved and the data on extract yield. The extract significantly (p < 0.01) increased the Ca and P and Cr levels in OVX + EUOE group compared to those in OVX control. Moreover, the extract significantly (p < 0.01) increased macro-mechanical indices of trabecular bone in OVX+EUOE group, relative to those in OVX control.
Conclusion: The yield of Eucommia ulmoides Oliver extract has been successfully optimized using RSM. The extract exhibited strong effects on bone quality.
Keywords: Optimization, Eucommia ulmoides, Box–Behnken design, Response surface methodology, Bone loss, Gene
Bisleuconothine A protects periodontal tissue via the regulation of RANKL expression and infiltration of inflammatory cells
Authors: Wang Fang , Sun Ping , Sun Qiang 
Published: June 2020

Purpose: To investigate the protective effect of bisleuconothine A on periodontal tissue in rats and the mechanism involved.
Methods: Adult male Sprague Dawley rats (n = 32) weighing 180 - 200 g (mean weight, 190 ± 10 g) were randomly assigned to four groups of eight rats each: control group, periodontitis group, bisleuconothine A (50 mg/kg) group and bisleuconothine A (100 mg/kg) group. Rats in the treatment groups received bisleuconothine intraperitoneally for two weeks. Periodontitis was induced in the rats using standard procedures. Serum and tissue samples were used for biochemical analysis. Alveolar bone loss was measured in rat maxillae, while the activity of bone alkaline phosphatase (BALP) was determined in serum. Tumor necrosis factor α (TNF-α) interleukin-1β and interleukin-6 (IL-1β and IL-6) were determined in gingival tissue using enzyme-linked immunosorbent assay (ELISA) kit. Gene and protein expressions of receptor activator of nuclear factor kappa-Β ligand (RANKL), osteoprotegerin (OPG), and matrix metallopeptidase-9 (MMP-9) were measured in gingival tissue using real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting, respectively.
Results: Bisleuconothine A treatment significantly and dose-dependently reduced alveolar bone loss, as well as serum levels of TNF-α, IL-1β and IL-6, but increased BALP activity in periodontitis rats (p < 0.05). It also significantly and dose-dependently reduced mRNA expressions of RANKL and MMP-9, but significantly increased OPG mRNA expression (p < 0.05). Similarly, treatment with bisleuconothine A significantly and dose-dependently down-regulated RANKL, p-NF-kB, p-IkBα and iNOS proteins in gingival tissue of periodontitis rats (p < 0.05). The results of histopathological examination indicated that bisleuconothine A treatment significantly reversed histological changes in periodontal tissues of periodontitis rats. It also significantly reduced the degree of polymorphonuclear (PMN) cell infiltration in periodontal tissue.
Conclusion: The results obtained show that bisleuconothine A protects periodontal tissue via the regulation of RANKL expression and infiltration of inflammatory cells.
Keywords: Bisleuconothine A, Expression, Inflammation, Periodontitis, RANKL
Potentials of neuron-specific enolase as a biomarker for gastric cancer
Authors: Wang Lingyun , Hu Ruirui , Liu Hongmei , Li Wen , Zhou Lili , Liu Xiangyu , Ding Yuanyuan 
Published: June 2020

Purpose: To investigate the potentials of neuron-specific enolase (NSE) as a biomarker for gastric cancer (GC).
Methods: Gastric cancer (GC) patients (n = 412) who underwent gastrectomy were recruited over a 3- year period for this study. Their clinicopathological data such as age, sex, histological type, depth, tumor invasion, lymph node metastasis, and distant metastasis were analyzed. The patients were followed up for four years and the outcomes were also assessed. Histological changes in biopsies and levels of expression of NSE in biopsies and serum of patients were determined using immunohistochemical staining, western blotting and enzyme-linked immunosorbent assay (ELISA), respectively.
Results: Immunohistochemical staining showed that NSE was differentially expressed in the cytoplasm of GC cells. Histological changes in biopsies of patients in the overexpression group were not significantly different from those of patients in under-expression group (p > 0.05). In NSE overexpression group, the number of patients in early stage GC subgroup (n = 186, 86.10 %, T1) were significantly higher than that in advanced GC subgroup (n = 124, 62.20 % T2–T4) (p < 0.05). However, in NSE under-expression group, there were more patients in advanced GC subgroup (n = 72, 37.70 %) than in early GC subgroup (n = 30, 13.80 %) (p < 0.05). Patients in NSE overexpression group survived longer than those in NSE under-expression group (p < 0.05). The level of expression of NSE significantly decreased with increase in TNM stage (p < 0.05). There was no significant difference in serum NSE level between GC patients and healthy control (p > 0.05). The results of the correlation analysis indicated that NSE levels were positively associated with GC.
Conclusion: The results obtained in this study suggest that NSE could serve as a potential biomarker for GC.
Keywords: Biomarker, Gastric cancer, Neuron-specific enolase, Overexpression, TNM staging
Analgesic effect of flurbiprofen ester and its effect on serum inflammatory factors and Β-endorphin expression in rats with incision pain
Authors: Ye Xiaomin , Ye Xianhua , Ye Nannan 
Published: June 2020

Purpose: To study the analgesic effect of flurbiprofen ester in rats with incision pain, and its effect on serum inflammatory factors and β-endorphin expression.
Methods: Seventy-five (75) healthy rats with foot contraction threshold induced by basic mechanical stimulation were randomly assigned to control, model control and treatment groups. Flurbiprofen was administered in 3 doses: 5, 10 and 15 mg/kg. Then, 3 mL of ventricular blood was taken from anesthetized rats and the serum levels of tumor necrosis factor-α (TNF- α), interleukin-1 β, interleukin-6 and β-endorphin were measured. The expression of β-endorphin in the spinal cord of rats with lumbar enlargement and ARC was determined.
Results: The TNF- α, interleukin-1 β and interleukin-6 concentrations were significantly lower in treatment group than in model rats, and decreased with time and dose (p < 0.05). In the treatment group, the level of serum β-endorphin decreased with increase in dose at 1 h, but increased with increase in dose at 5 h and 10 h (p < 0.05). The levels of β-endorphin in the spinal cord, was significantly lower in model rats than in control rats (p < 0.05).
Conclusion: Pre-administration of flurbiprofen ester reduces serum inflammatory factors and upregulates β-endorphin expression in rats with incision pain. Thus, it flurbiprofen exerts analgesic effect.
Keywords: Flurbiprofen ester, Incision pain, Rat, Analgesia, Inflammatory factor, β-endorphin
Synergistic effect of a combination of granulocyte macrophage colony-stimulating factor and thymosin α1 on Lewis lung cancer transplanted tumor in mice
Authors: Lan Haiyan , Hao Yanrong , Lv Yanru , Li Guangyu , Mo Yuncong , Zheng Cheng , Yuanfa Li 
Published: June 2020

Purpose: To study the synergistic effect of a combination of granulocyte-macrophage colonystimulating factor and thymosin-α1 on the treatment of Lewis lung cancer transplanted tumor.Methods: C57BL/6 mice were used. A mouse model of Lewis lung cancer was established using Lewis lung cancer cell lines. The mice were randomly divided into blank control group, polyene taxol (DTX) group, DTX thymosin α1 (Tα-1) group, and DTX granulocyte-macrophage colony-stimulating factor (GM-CSF) group, with 8 mice per group. The degree of tumor inhibition, thymus mass, thymus index, spleen mass, spleen index, IL-6, TNF-1, IFN-1, CD4+, CD8+ T cells and the ratio of CD4+/CD8+ were determined by ELISA and flow cytometry.Results: Body mass, thymus mass, thymus index, spleen mass, spleen index, IL-6, TNF-1, IFN-1, CD4+, CD8+ T cells and the ratio of CD4+/CD8+ in DTX + Tα-1 group, DTX + GM-CSF group and DTX + Tα-1 + GM-CSF group were significantly elevated (p < 0.05), relative to the corresponding levels in DTXmice (p < 0.05). Body mass, degree of tumor inhibition, thymus mass, thymus index, spleen mass, spleen index, IL-6, TNF-1, IFN-1, CD4, CD8 T cells and CD4+/CD8+ ratio in DTX + Tα-1 + GM-CSF mice were significantly elevated, relative to the DTX + Tα-1 and DTX + GM-CSF groups (p < 0.05). Thestate of the tumor was significantly improved in the DTX + Tα-1 and DTX + GM-CSF mice.Conclusion: A combination treatment of GM-CSF, Tα-1 and DEX effectively enhances the resistance of mice and suppresses chemotherapy-induced decrease in body weight. This finding may be of clinical significance.
Keywords: Granulocyte macrophage, Colony-stimulating factor, Thymosin, Docetaxel, Lewis lung cancer, Transplanted tumor
Knowledge, attitude and practice of Lebanese community pharmacists with regard to self-management of low back pain
Authors: Dabbous Mariam K. , Moustafa Sara M. , Sakr Fouad R. , Akel Marwan G. , Safwan Jihan H. , Cherfan Michelle M. , Rahal Mohamad K. 
Published: June 2020

Purpose: To determine the knowledge, attitude and reported practice of Lebanese community pharmacists who advise persons who present with low back pain.Methods: This was a multi-center cross-sectional study conducted in over 300 community pharmacies across Lebanon from December 2017 to May 2018. Pharmacists working at a community pharmacy were considered eligible, and those who volunteered to participate completed the questionnaire. The questionnaire was designed for self-completion by the pharmacist and included demographic questions about the respondent, questions that assessed knowledge and attitude toward low back pain, and questions about treatment to reflect and characterize the nature of practice. The primary outcome was to determine the knowledge, attitude and reported practice of the Lebanese pharmacists advising people who presented with low back pain. The secondary outcome was to assess factors that affect knowledge, attitude, and practice.Results: The response of 320 community pharmacists was analysed. The proportion of pharmacists with good knowledge about low back pain (51. 7 %) was slightly higher than those with poor knowledge (48. 3 %). Oral therapy was the most prescribed dosage form for back pain compared to local patch and cream. Among oral dosage forms, non-steroidal anti-inflammatory drugs were the most prescribed medications (42 %). Of the patients’ referral to the physician if necessary, 73.1 % of the referrals were by pharmacists.Conclusion: Community pharmacists in Lebanon demonstrate an acceptable level of knowledge of back pain, yet major gaps still exist, particularly in terms of the quality of advice. Hence, more education is needed to provide better quality of advice.
Keywords: Attitude, Knowledge, Low back pain, Reported practice, Quality of advice
Carboprost tromethamine prevents caesarean sectionassociated postpartum hemorrhage
Authors: Wang Li , Jiang Hong-mei , Yang Rui-rui 
Published: June 2020

Purpose: To investigate the effect of carboprost tromethamine on post-partum hemorrhage associated with caesarean section.Methods: One hundred patients with postpartum hemorrhage induced by cesarean section who were admitted to Binzhou People’s Hospital from October 2016 to August 2018 were selected. They were randomly assigned to two groups: control group and treatment group. Patients in the control group were administered oxytocin, while those in the treatment group received oxytocin in combination with carboprost tromethamine. The incidence of hemorrhage and adverse reactions were compared for the two groups.Results: The two groups showed gradually increasing degrees of postpartum hemorrhage within 24 h, but the treatment group had significantly lower volume of postpartum hemorrhage than the control group at different time points (p < 0.05). At the 1st, 3rd and 5th days after delivery, the height of the uterine fundus decreased gradually in the two groups, but was smaller in the treatment group than in the oxytocin group at all time points (p < 0.05). Total response in the treatment group was 98 %, which was significantly higher than 78 % in the control group (p < 0.05). The incidence of adverse reactions in the treatment group (12 %) was significantly lower than that in the control group (24 %, p < 0.05).Conclusion: Carboprost tromethamine prevents postpartum hemorrhage after cesarean section. It effectively reduces bleeding and promotes uterine involution, without obvious adverse reactions.
Keywords: Carboprost tromethamine, Oxytocin, Cesarean section, Postpartum hemorrhage
ENTREVISTA COM A ESCRITORA SURDA SHIRLEY VILHALVA
Authors: Vilhalva Shirley 
Published: June 2020

Shirley Vilhalva, nascida em Campo Grande, estado de Mato Grosso do Sul, no ano de 1964, pedagoga, professora da Universidade Federal do mato Grosso do Sul – UFMS. Escritora e poeta surda, tem contribuído com a Comunidade Surda a partir do desenvolvimento de diversos projetos de pesquisa e de divulgação da Cultura Surda. Estreia na Literatura com a publicação do livro Despertar do Silêncio, no ano de 2004, de cunho autobiográfico, suscita profundas reflexões acerca dos desafios e conquistas das pessoas surdas no Brasil, não deixando de proporcionar momentos de verdadeira fruição e deleite.
Em meio ao isolamento causado pela crise mundial causada pela pandemia do coronavírus, concedeu-me, gentilmente, esta entrevista, na qual discute aspectos da Literatura Surda e de seu particular processo criativo. A entrevista foi concedida no dia 24 de abril, dia em que se comemoram os 18 anos da lei 10.436 que reconheceu a Libras como segunda língua oficial do Brasil, sendo a primeira língua para a comunidade surda.
Development of Frequency GSM Signal Jammer
Authors: Arowolo Oluwole , Adekunle Adefemi 
Published: June 2020

This paper gives an explanation to the concept of mobile Jamming and explores jamming in the two popular mobile networks: Global system for mobile communication (GSM) and digital cellular network (DCS). The designed intelligent jamming system blocks the controller channel only, also it operates only if an active mobile is in the controlled area. The ADS - advance Design System for Agilent software package is used analyzed and simulated the mobile jamming system. The Mobile Detector Phone Jammer successfully jammed all the four operators but the radius of the range did not get as expected in the designed, this project only focuses on blocking the signal transmission between the ranges 935 to 960 MHz when tested and performed well. This can be effectively find its use in School Examination hall and religion worship centers. Keywords—Jammer, Mobile Phone, Network, Signal, Frequency