bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Use of Innominate Artery Cannulation for Antegrade Cerebral Perfusion in Aortic Dissection
Authors: Payabyab Eden C , Hemli Jonathan M. , Mattia Allan , Kremers Alex , Vatsia Sohrab K. , Scheinerman S. Jacob , Mihelis Efstathia A , Hartman Alan R. , Brinster Derek R. 
Published: July 2020

Abstract
Background: Direct cannulation of the innominate artery for selective antegrade cerebral perfusion has been shown to be safe in elective proximal aortic reconstructions. We sought to evaluate the safety of this technique in acute aortic dissection. Methods: A multi-institutional retrospective review was undertaken of patients who underwent proximal aortic reconstruction for Stanford type A dissection between 2006 and 2016. Those patients who had direct innominate artery cannulation for selective antegrade cerebral perfusion were selected for analysis. Results: Seventy-five patients underwent innominate artery cannulation for ACP for Stanford Type A Dissections. Isolated replacement of the ascending aorta was performed in 36 patients (48.0%), concomitant aortic root replacement was required in 35 patients (46.7%), of whom 7 had a valve-sparing aortic root replacement, ascending aorta and arch replacement was required in 4 patients (5%). Other procedures included frozen elephant trunk (n = 11 (14.7%)), coronary artery bypass grafting (n = 20 (26.7%)), and peripheral arterial bypass (n = 4 (5.3%)). Mean hypothermic circulatory arrest time was 19 ± 13 minutes. Thirty-day mortality was 14.7% (n = 11). Perioperative stroke occurred in 7 patients (9.3%).Conclusions: This study is the first comprehensive review of direct innominate artery cannulation through median sternotomy for selective antegrade cerebral perfusion in aortic dissection. Our experience suggests that this strategy is a safe and effective technique compared to other reported methods of cannulation and cerebral protection for delivering selective antegrade cerebral perfusion in these cases.
Pre- and Postoperative Parameters on Magnetic Resonance Imaging Predict Continence Recovery after Laparoscopic Radical prostatectomy
Authors: Zhang Fan , Yang Bin , Yan Ye , Hao Yichang , Huang Yi , Ma Lulin 
Published: July 2020

Abstract
Background: To evaluate the association between pre- and postoperative parameters on magnetic resonance imaging (MRI) and continence recovery after laparoscopic radical prostatectomy (LRP). To develop a risk scoring system for predicting continence recovery after LRP. Methods: 73 patients who underwent LRP were retrospectively reviewed. Demographic characteristics, clinicopathologic outcomes and several MRI parameters before and after surgery were evaluated. Continence was defined as no pad per day. Early continence recovery was defined as continence recovery within 3 months. Kaplan-Meier analyses and log-rank test were used to compare time to continence recovery. Cox proportional-hazards regression analyses were performed to identify independent predictors of continence recovery. Results: Patients with smaller prostatic volume, shorter intravesical prostatic protrusion length (IPPL), shorter preoperative membranous urethral length (MUL), lower MUL-removal rate, triangular vesicourethral anastomosis (VUA) and neurovascular bundle sparing experienced a faster continence recovery (All, p < 0.05). Multivariate analyses revealed IPPL (hazard ratio [HR]: 0.94, p = 0.044), preoperative MUL (HR: 1.10, p = 0.032), MUL-removal rate (HR: 0.91, p = 0.007) and shape of VUA (square vs. triangle, HR: 2.30, p = 0.012) were independent predictors of continence recovery. The four parameters were therefore used to develop a risk scoring system, termed Post-Prostatectomy Incontinence Score (PPIS) and ranging from 0 to 4. We observed early continence recovery in 100%, 84.6%, 27.8%, 0% and 0% of patients with a PPIS of 0, 1, 2, 3, 4, respectively. Conclusion: IPPL, preoperative MUL, MUL-removal rate and shape of VUA were independently predictors of continence recovery. PPIS could accurately predict the early continence recovery after LRP.
Molecular characterization of Enterobacteriaceae producing β-lactamase and methicillin-resistant staphylococci isolated from the hospital environment and catheters in two public hospitals in Benin
Authors: GBOTCHE Elodie , DOUGNON Victorien Tamègnon , CHABI Yossounon , AGBANKPE Jerrold , DEGUENON Esther , SEDAH Paulin , FABIYI Kafayath , BABA-MOUSSA Lamine , AGBANGLA Clément , BANKOLE Honoré 
Published: July 2020

Abstract
Background: Antimicrobial resistance is a real public health problem. All over the world, it has a considerable impact in hospitals. The present study aims to analyze the presence of resistance genes and bacterial ecology in two hospitals in Benin. Methods: To do this, 146 environmental and catheter samples were collected at the University Hospital Center of Abomey-Calavi / So-Ava and at the Beninese Army Hospital. These samples were inoculated on Mannitol Salt and Eosin Methylene Blue agars. The colonies obtained were identified and their sensitivity to antibiotics was tested, using the usual bacteriological techniques. Four resistance genes encoding the production of extended spectrum beta-lactamases (blaCTX-M1, blaCTX-M2, blaCTX-M9, blaCTX-M15) have been searched in the genome of enterobacteriaceae strains. At the level of staphylococci, the gene coding for methicillin resistance (mecA) was sought.Results: At the end of this study, 69 strains of enterobacteria and 60 of staphylococci were identified. A predominance of Staphylococcus aureus (25.6%) followed by Enterobacter cloacae (21.0%) and coagulase negative staphylococci (21.0%) was noted. These bacterial strains showed to be multidrug-resistant, particularly to beta-lactams, fluoroquinolones, aminoglycosides and macrolides. Beta-lactamases were identified in the genome of bacterial strains with a predominance of blaCTX-M15 (42.8%). The frequency of the mecA gene in staphylococci was 50%.Conclusions: These results show the magnitude of the antimicrobial resistance situation in hospitals. They can be used to support advocacy for urgent action at the national level, especially with regard to the management and efficient use of antimicrobials in Benin.
Donor’s Motivation towards Providing Aid for Sustainable Initiatives: An Analysis of the Global Scenario
Authors: Irfan Zareena Begum , Baswapoor Srihitha 
Published: July 2020

Abstract
A substantial number of studies on development aid have explored the reasons for donor’s aid allocation decisions, but very few exist for the Sustainable Production and Consumption (SP&C) sector. This fails to provide evidence to policymakers to evaluate regarding inquiries about their allocative efficiency, equal allocation & rhetoric with regard to international commitment of Sustainable Production and Consumption. This study examines donors’ motivations to provide aid for the sustainable initiatives in general and sustainable production and consumption sector in particular using 35 donor and receptor countries worldwide between the time period 1992 and 2016. By examining four rational choice models, the results reveal that donors take into consideration their own self-interests as well as they cater to the recipients’ needs for allocation of aid. The study also finds that, more aid is given to countries with good governance capabilities and thus do not prioritize fragile states.
Janus 3D printed dynamic scaffolds for nanodeflection-driven bone regeneration
Authors: Camarero-Espinosa Sandra , Moroni Lorenzo 
Published: July 2020

Abstract
The application of physical stimuli to cell cultures has shown potential to modulate multiple cellular functions including migration, differentiation and survival. However, the relevance of these in-vitro models to future potential extrapolation in-vivo depends on whether stimuli can be applied “externally”, without invasive procedures. Here, we report on the fabrication and exploitation of dynamic additive-manufactured Janus scaffolds that are activated “on-command” via external application of ultrasounds, resulting in a mechanical nanodeflection that is transmitted to the surrounding cells. Janus scaffolds were spontaneously formed via phase-segregation of biodegradable polycapolactone (PCL) and polylactide (PLA) blends during the manufacturing process and behave as ultrasound transducers (acoustic to mechanical) where the PLA and PCL phases represent the active and backing materials, respectively. Remote stimulation of Janus scaffolds led to enhanced cell proliferation, matrix deposition and osteogenic differentiation of seeded bone marrow derived stromal cells (BMSCs) via formation and activation of voltage-gated calcium ion channels.
Actual time-series of single-cell transcriptomics reveals circadian clocks as key regulators of cell differentiation in Arabidopsis
Authors: Torii Kotaro , Inoue Keisuke , Bekki Keita , Haraguchi Kazuya , Kubo Minoru , Kondo Yuki , Suzuki Takamasa , Kubota Akane , Uemoto Kyohei , Shimizu Hanako , Saito Masato , Fukuda Hiroo , Araki Takashi , Endo Motomu 
Published: July 2020

Abstract
The basis of toti/pluripotency is elaborate regulation of cell-cycle progression and cell-fate determination. Circadian clocks are involved in this process, but the underlying mechanisms have not been studied due to technical limitations. In particular, there is a lack of research on the universality of cell differentiation mechanisms in multicellular organisms using plants with high cell-fate plasticity. Here, exploiting in vivo single-cell RNA sequencing and a new actual time reconstitution method, PeakMacth, we analyzed actual time-series of cell reprogramming and differentiation processes at single-cell resolution, and found that the circadian clock modulates cell differentiation via BES1-mediated GSK3 signaling, which has a β-catenin-like function in Arabidopsis. In this pathway, the clock gene LUX in meristematic stem cells directly targets the CYCD and RETINOBLASTOMA-RELATED (RBR) genes, which are commonly involved in cell-cycle progression and cell-fate determination in plants and animals. In addition, the rhythmicity of the circadian clock was associated with cell state, and the establishment of the circadian rhythm preceded cell differentiation. Thus, our study not only reveals the involvement of the circadian clock in the differentiation of plant stem cells but also demonstrates functionally analogous features in the regulatory system of cell differentiation across species.
A biolabel research based on metabonomics reveals the therapeutic potentials of Herba Lysimachiae in synovial diseases
Authors: LI Xu-zhao , ZHANG Shuai-nan , LIU Qi , WANG Yu-mei 
Published: July 2020

Abstract
Background: Previous research has demonstrated that Herba Lysimachiae (HL) exerts the dual effects on platelet aggregation in the synovium, which may contribute to its protection against synovial lesions under different situations. However, the mechanism is unclear. In the present experiment, a biolabel research based on metabonomics was used to mine more information about the intervention of HL on synovium at the metabolite level, which may help to analyze the regulation of HL on synovial platelet aggregation and its possible treatment in synovial diseases. Methods: Synovial metabolic profiling was analyzed using a Shimadzu Nexera UHPLC LC-30A system and an AB SCIEX Triple TOF 4600 mass spectrometer. Enzyme-linked immunosorbent assay was used to verify the biolabels analysis results. Results: Totally, thirteen common metabolites were differentially expressed after treating with HL, and implicated in 2 key pathways (arachidonic acid metabolism and glycerophospholipid metabolism). ELISA showed that HL up-regulated the expression of prostaglandins E1 and E2 in synovial tissues. Conclusions: This study reveals that HL may regulate the synovial platelet aggregation through prostaglandin E1/E2. Additionally, HL is suitable for treating synovial diseases, especially osteoarthritis, which may be associated with the platelet aggregation, apoptosis, inflammation, angiogenesis, and carcinogenesis processes.
Comparison of Mycobacterium Avium Subsp. Paratuberculosis (MAP) Infection in Cattle, Sheep and Goats Following the Detection of Clinical Cases: Fact and Fiction
Authors: Borujeni Mahdi Pourmahdi , Hajikolaei Mohammad Rahim Haji , Ghorbanpoor Masoud , Sahar Hamzeh Elhaei , Bagheri Saeed , Roveyshedzadeh Sanaz 
Published: July 2020

Abstract
Background: Paratuberculosis or Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is chronic infectious granulomatous enteritis of ruminants, which has a worldwide occurrence. The most common symptoms of this disease in cattle are loss of milk production, weight loss, and diarrhea and in sheep and goats are emaciation, anorexia and sever disability. The aim of this study was to compare the seroprevalence of MAP in cattle, sheep and goats following the detection of clinical cases in Southwest of Iran. Blood samples were randomly collected from 530 cattle, 568 sheep and 368 goats in Southwest of Iran. Sera were tested by a commercial ELISA kit for detection of antibodies to MAP. Results: Overall apparent and true seroprevalence rate of MAP was 6% (95% CI: 4.9 – 7.3 percent) and 11.36% (95% CI: 9.87 – 13.07 percent), respectively. Apparent and true seroprevalence of MAP were 4.34% (95% CI: 2.88 – 6.46 percent) and 7.59% (95% CI: 5.61 – 10.19 percent), respectively in cattle, 6.87% (95% CI: 5.05 – 9.27 percent) and 13.34% (95% CI: 10.78 – 16.4 percent), respectively in sheep, and 7.07 %( 95% CI: 4.82 – 10.18 percent) and 13.68% (95% CI: 10.52 – 17.58 percent), respectively in goats. As a result, there was no significant relationship between species and MAP infection. Moreover, multivariate logistic regression showed that the infection rate is not associated with age, gender, and geographical location in cattle, sheep and goats (P>0.05). Conclusions: This study confirms that the seroprevalence of MAP following the detection of clinical cases is considerable in the cattle, sheep and goats in Southwest of Iran, although in cattle it is less than goats and sheep. Therefore, preventive and control measures should be considered by animal health authorities and meat and dairy processing units.
Unilateral Tubular Approach for Bilateral Laminotomy vs Transforaminal Lumbar Interbody Fusion for Lumbar Spinal Stenosis
Authors: Lv Bin , Hu Minjie , Zhang Lei , Bi Shiqi 
Published: July 2020

Abstract
Background: Endoscopic techniques is considered to be the standard tissue sparing approach for surgical advantages, rapid rehabilitation, and instability prevention. PEID techniques were introduced to treat lumbar spinal stenosis. However, its effectiveness and safety in the treatment of moderate and severe lumbar stenosis is uncertain.Methods: Between June 2014 and June 2016, patients suffering from lumbar spinal stenosis underwent a bilateral laminotomy through a unilateral tubular approach. Demographics, intraoperative data, and patient-reported outcomes were ascertainedResults:There were no significant differences in patient demographics between the two groups. The statistically significant improvements in leg and back VAS scores were reported on the ipsilateral and the contralateral side (p<0.05, respectively). Analysis of VAS and ODI scores demonstrated no significant differences in improvement on both groups. There were no difference in radiographical and intraoperative outcomes during the follow-up period. Conclusions: Regarding the intraoperative data, and patient-reported outcomes, the PEID is a safety, and efficacy minimally invasive technique. This observation suggests that the PEID techniques were excellent or showed good results.
Early complications of preoperative external traction fixation in staged treatment of tibial fractures: a 402 cases series study
Authors: Yang Jiazhao , Zhu Wanbo , Dong Qirong 
Published: July 2020

Abstract
Background and Objective: Different external skeletal fixators have been widely used in preoperative traction of high-energy tibial fractures prior to a definitive surgical treatment. However, early complications associated with this staged treatment for traction and soft tissue injury recovery have rarely been discussed. This study aimed to analyze the early complications associated with preoperative external traction fixation in the staged treatment of tibial fractures.Materials and Methods: A total of 402 patients with high-energy tibial fractures treated using preoperative external traction fixation at a level-1 trauma center from 2014 to 2018 were enrolled in this retrospective study. Data regarding the demographic information, Tscherne soft tissue injury, fracture site, entry point placement, and duration of traction were recorded. Procedure-related complications such as movement and sensation disorder, vessel injury, discharge, infection, loosening, and iatrogenic fracture were analyzed.Results: The mean patient age was 42.5 (18–71) years and the mean duration of traction was 7.5 (0–26) days. In total, 19 (4.7%) patients presented with procedure-related complications, including technique-associated complications in 6 patients and nursing-associated complications in 13. Differences in the incidence of complications with respect to sex, affected side, soft tissue injury classification, and fracture sites were not observed. However, the number of complications due to hammer insertions was significantly fewer than those due to drill insertions (2.9% vs. 7.4%).Conclusion: The application of preoperative external traction fixation had been proved to be an ideal alternative treatment for high energy tibial fractures. In this study, we found the incidence of early complications of the fixation is low, and it is not significantly associated with the severity of soft tissue injury and fracture site. Although relatively rough and more likely to cause pain, complications of hammer insertions were significantly fewer than drill insertions. The possible reason was higher probability of heat damage and loosening by drilling.
The role of complete TURBT before radical cystectomy for organ-confined bladder cancer
Authors: Liang Hai-tao , Chen Zhuang-fei , Xu Zhan-ping , Lin Zhi-jun , Qin Zi-ke , Ye Yunlin 
Published: July 2020

Abstract
ObjectiveTo investigate the role of complete transurethral resection of bladder tumor (TURBT) before radical cystectomy.Patients and MethodsPatients who underwent radical cystectomy in our center from January 2008 to December 2018 were retrospectively reviewed. Those with disease >T2N0M0 or positive surgical margin and those who were administrated neoadjuvant/adjuvant chemotherapy or radiotherapy were excluded. Complete TURBT was defined as no visible lesion under endoscopic examination after TURBT, presence of muscle layer in the specimen or no tumor in a specimen separated from the base. Kaplan–Meier and log-rank tests assessed disease-free survival. Logistic and Cox regression analysis were performed to identify potential predictors.ResultsIn total, 236 patients were included, and 207 patients were male. The median age was 61 years old. The median number and size were 1 and 3cm respectively, and maximal pathological T stage was T2 in 94 patients. Complete TURBT was related to tumor size (p=0.041), histological variants (p=0.026) and downstaging (p<0.001). Tumor size, grade and histological variants were independent predictors of complete TURBT. With a median follow-up of 42.7 months, 30 patients experienced recurrence. Age and histological variants were independent predictors of disease-free survival (p=0.027 and 0.041, respectively). Complete TURBT was associated with poor prognosis, however, the result was not significant (p=0.068). Downstaging was not associated with survival outcome.ConclusionsComplete TURBT is related to an increased rate of downstaging before radical cystectomy, and both of these factors did not improve oncological outcomes for patients with organ-confined bladder cancer.
Identification Of Both Shared And Specific Potential Molecular Mechanisms Of Arvc And Dcm Based On A Genome-Wide Microarray Dataset
Authors: Zhang Tang , Guan Yao-Zong , Liu Hao 
Published: July 2020

Abstract
Background: The study aimed to detect the shared differentially expressed genes (DEGs) and specific DEGs of arrhythmogenic right ventricular cardiomyopathy (ARVC) and dilated cardiomyopathy (DCM) as well as their pathways.Methods: The GSE29819 dataset was examined for the DEGs of ARVC vs. non-failing transplant donor hearts (NF), DCM vs. NF, and ARVC vs. DCM based on 6 patients with ARVC, 7 patients with DCM, and 6 non-failing transplant donor hearts that were never actually transplanted. The shared DEGs and specific DEGs were screened out using a Venn diagram. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, Gene Ontology (GO) annotation, and protein-protein interaction (PPI) of the DEGs were determined using online analytical tools. Then, the modules and hub genes were identified using Cytoscape software.Results: A total of 684 shared DEGs of ARVC vs. NF and DCM vs. NF, 1371 specific DEGs of ARVC vs. NF, and 1075 specific DEGs of DCM vs. NF were identified. The shared DEGs were enriched in 63 biological processes (BP), 11 molecular functions (MF), 10 cellular components (CC), and 25 KEGG pathways. The DEGs of ARVC vs. DCM were enriched in 71 BPs, 19 MFs, 14 CCs, and 26 KEGG pathways. A PPI network with 187 nodes, 700 edges, and 2 modules, and another PPI network with 575 nodes, 2834 edges, and 7 modules were constructed based on the shared and specific DEGs, respectively. The top ten hub genes CCR3, CCR5, CXCL2, CXCL10, CXCR4, FPR1, APLNR, PENK, BDKRB2, GRM8, and RPS8, RPS3A, RPS12, RPS14, RPS21, RPL14, RPL18A, RPL21, RPL31 were identified for the shared and specific PPI networks, respectively.Conclusions: Our findings may help further the understanding of both shared and specific potential molecular mechanisms of ARVC and DCM.
Barriers to Sexual and Reproductive Health Information among University Students in Uganda: A qualitative study
Authors: Nuwamanya Elly , Qureshi Eman T.U. , Pemunta Ngambouk Vitalis , Adedze Miranda , Nalwanga Robinah , Nuwasiima Afra , Babigumira Janet U. , Asiimwe Francis T. , Babigumira Joseph B. 
Published: July 2020

Abstract
Background: University students are one of the most vulnerable groups to sexual and reproductive health (SRH) threats, yet they often have limited access to SRH tools, services and information. This study explored university students’ perceptions of SRH, their source of SRH information and how they authenticated such information in Uganda. Methods: Data were collected from 40 students of Kyambogo university—20 males and 20 females through 4 gender-classified Focus Group Discussions (FGDs). Atlas ti 8 software was used to analyse the data and generate themes from interview transcripts.Results: Many students perceived SRH to be about sexual intercourse and its related consequences. Perceived risky behaviours included mainly having multiple sexual partners. The students identified the reduced sexual sensitivity, struggle to bear children in the future, as the myths and misconceptions about different SRH goods and services in addition to the uncertainty over safe days. The various sources of SRH information included Google, social media, health centres, friends, parents and government and non-government organizations. The main barriers to accessing SRH information included lack of finances, inadequate or few medical personnel, and service provider bias. Many of them authenticated the SRH information from the internet through their friends. Conclusion: New approaches and interventions should target both students and their parents, as this multifaceted approach will reduce the societal stigma, bias, ignorance, negative attitudes, and exposure to risky sexual behaviours among such youth populations.