bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthetic protein condensates that recruit and release protein activity in living cells
Authors: Yoshii Tatsuyuki , Yoshikawa Masaru , Ikuta Masahiro , Tsukiji Shinya 
Published: November 2020

AbstractCompartmentation of proteins into biomolecular condensates or membraneless organelles formed by phase separation is an emerging principle for the regulation of cellular processes. Creating synthetic condensates that accommodate specific intracellular proteins on demand would have various applications in chemical biology, cell engineering and synthetic biology. Here, we report the construction of synthetic protein condensates capable of recruiting and/or releasing proteins of interest in living mammalian cells in response to a small molecule or light. We first present chemogenetic protein-recruiting and -releasing condensates, which rapidly inhibited and activated signaling proteins, respectively. An optogenetic condensate system was successfully constructed that enables reversible release and sequestration of protein activity using light. This proof-of-principle work provides a new platform for chemogenetic and optogenetic control of protein activity in mammalian cells and represents a step towards tailor-made engineering of synthetic protein condensates with various functionalities.
Contagious Antibiotic Resistance: Plasmid Transfer Among Bacterial Residents of the Zebrafish Gut
Authors: Loftie-Eaton Wesley , Crabtree Angela , Perry David , Millstein Jack , Robinson Barrie , Forney Larry , Top Eva 
Published: November 2020

AbstractBy characterizing the trajectories of antibiotic resistance gene transfer in bacterial communities such as the gut microbiome, we will better understand the factors that influence this spread of resistance. Our aim was to investigate the host network of a multi-drug resistance broad-host-range plasmid in the culturable gut microbiome of zebrafish. This was done throughin vitroandin vivoconjugation experiments withEscherichia colias donor of the plasmid pB10::gfp. When this donor was mixed with the extracted gut microbiome, only transconjugants ofAeromonas veroniiwere detected. In separate matings between the same donor and four prominent isolates from the gut microbiome, the plasmid transferred to two of these four isolates,A. veronii and Plesiomonas shigelloides,but not toShewanella putrefaciensandVibrio mimicus. When theseA. veronii and P. shigelloidestransconjugants were the donors in matings with the same four isolates, the plasmid now also transferred fromA. veroniitoS. putrefaciens.P. shigelloideswas unable to donate the plasmid andV. mimicuswas unable to acquire it. Finally, when theE. colidonor was addedin vivoto zebrafish through their food, plasmid transfer was observed in the gut but only toAchromobacter sp.,a rare member of the gut microbiome. This work shows that the success of plasmid-mediated antibiotic resistance spread in a gut microbiome depends on the donor-recipient species combinations and therefore their spatial arrangement. It also suggests that rare gut microbiome members should not be ignored as potential reservoirs of multi-drug resistance plasmids from food.ImportanceTo understand how antibiotic resistance plasmids end up in human pathogens it is crucial to learn how, where and when they are transferred and maintained in members of bacterial communities such as the gut microbiome. To gain insight into the network of plasmid-mediated antibiotic resistance sharing in the gut microbiome, we investigated the transferability and maintenance of a multi-drug resistance plasmid among the culturable bacteria of the zebrafish gut. We show that the success of plasmid-mediated antibiotic resistance spread in a gut microbiome can depend on which species are involved, as some are important nodes in the plasmid-host network and others dead-ends. Our findings also suggest that rare gut microbiome members should not be ignored as potential reservoirs of multi-drug resistance plasmids from food.
EphB6 regulates TFEB-lysosomal pathway and survival of disseminated quiescent breast cancer cells
Authors: Zangrossi Manuela , Chakravarty Probir , Romani Patrizia , Ratcliffe Colin D.H. , Hooper Steven , Dori Martina , Forcato Mattia , Bicciato Silvio , Dupont Sirio , Sahai Erik , Montagner Marco 
Published: November 2020

AbstractLate relapse of disseminated cancer cells is a common feature of some types of tumors. Several intrinsic and extrinsic factors have been shown to affect reawakening of disseminated dormant cancer cells (DDCCs); however, the signals and processes sustaining survival of DDCCs in a foreign environment are still poorly understood. We have recently shown that crosstalk with lung epithelial cells promotes persistence of DDCCs from estrogen receptor positive (ER+) breast tumors. Here we show that TFEB-lysosomal axis is activated in DDCCs and that it is modulated by the pro-survival ephrin receptor EphB6. TFEB lysosomal direct targets are enriched in DDCCsin vivoand correlate with relapse in ER+ breast cancer patients. Direct contact of DDCCs with alveolar type I-like lung epithelial cells drives lysosomal accumulation and EphB6 induction. EphB6 contributes to TFEB transcriptional activity and lysosome formation in DDCCsin vitroandin vivo, and supports survival of DDCCs in coculture andin vivo. Furthermore, signaling from EphB6 promotes the proliferative response of surrounding lung parenchymal cellsin vivo.
Sexual harassment and organizational behaviors: the moderating effect of power distance
Authors: Choi Youngkeun 
Published: November 2020

PurposeBased on the cognitive appraisal theory, the current research extended the content domain of sexual harassment research by examining the moderating effect of power distance orientation, a cultural value on the relationship between sexual harassment and organizational behavior.Design/methodology/approachFor this, this study used a survey method and multiple regression analyses with 285 Korean employees.FindingsAs a result, first, the more unwanted sexual attention employees perceived from their leaders, the less likely they were to be affectively committed to their organization. Second, the more sexual coercion employees perceived from their leaders, the more likely they were to have turnover intention. Third, the negative relationship between unwanted sexual attention and affective commitment is stronger for the employees who are low rather than high in power distance orientation. Finally, the positive relationship between sexual coercion and turnover intention is stronger for the employees who are low rather than high in power distance orientation.Originality/valueThe study expanded the content area of sexual harassment research by showing the control effect of the power distance direction.
Interaction profile of a mixed-culture fermentation of Issatchenkia orientalis and Saccharomyces cerevisiae by transcriptome sequencing
Authors: Zhang Wenwen , Weng Peifang , Wu Zufang 
Published: November 2020

PurposeMixed fermentation with Saccharomyces cerevisiae and non-Saccharomyces yeasts has become an oenlogical tool to improve wines’ organoleptic properties. However, the maximum utilization of this method is dependent upon understanding the influence of mixed cultures on the physiology of S.cerevisiae and non-Saccharomyces yeasts.Design/methodology/approachIn this study, the supernatants from 48 h mixed-culture fermentation were added to the pure cultures of Issatchenkia orientalis and Saccharomyces, respectively. And the authors used RNA sequencing to determine the transcriptome change of I.orientalis and S.cerevisiae in a mixed culture.FindingsThe results showed that multiple genes associated with cell growth and death were differentially expressed. Genes related to biosynthesis of amino acids were enriched among those upregulated in the mixed-fermentation supernatant. Meanwhile, the differential expression level of genes encoding enzymes essential for formation of aroma compounds was found in the single and in the mixed fermentation. The high expression level of molecular chaperones Hsp70, Hsp90 and Hsp110 suggests that metabolites of mixed-culture fermentation may lead to aggregation of misfolded proteins. Moreover, upregulation of ethanol dehydrogenase I ADH1 in the mixed-culture fermentations was highlighted.Originality/valueThis is the first time that RNA-seq was used to analyze changes in the transcriptome of mixed cultures. According to the results the authors’ manuscript provided, an integrated view into the adaptive responses of S.cerevisiae and non-Saccharomyces yeasts to the mixed-culture fermentation is benefit for the potential application of S.cerevisiae and non-Saccharomyces yeasts in fruit wine brewing.
Explaining business excellence in Chinese firms: how does institutional relatedness matter?
Authors: Yan Haifeng , Wang Qihu , Ke Yi , Wang Juan 
Published: November 2020

PurposeIt is widely accepted that business excellence comes from firm-specific factors. However, it is still unclear how institutional relatedness – the degree of embeddedness with the dominant institutions that confer resources and legitimacy, influences the business excellence of the firm. The purpose of this study is to explore the influence of three kinds of institutional relatedness, i.e. home government ties, initial public offerings (IPOs) and alliances with foreign firms, on the business excellence of Chinese firms.Design/methodology/approachThis study uses a sample of firms enlisted on the “Most Respected Companies” rank in China during the period 2002–2015 and their paired firms who are absent from the list, by means of ordinary least square regression estimator, to explore the relationship between institutional relatedness and business excellence.FindingsThe empirical results suggest that IPOs and alliances with foreign firms significantly strengthen firms’ business excellence. Furthermore, home government ties have positive effects on outbound IPOs and alliances with foreign firms but hinder business excellence.Originality/valueThis study extends the business excellence literature by characterizing institutional rather than firm-specific factors from an institution-based view. It also enriches research on outcomes of institutional relatedness through investigating empirically its impact on business excellence. The findings provide new insights into the dual role of home government ties in achieving business excellence.
Can a region’s network location characteristics affect its innovation capability? Empirical evidence from China
Authors: Han Minrui , Sun Bing , Su Xiao 
Published: November 2020

PurposeThis study aims to explore the influence of a region’s network location characteristics and indirect connections on its innovation capability. The aim is to assist regions in different network locations to use innovation resources to improve their innovation capabilities more efficiently.Design/methodology/approachThis paper represents the Chinese regional innovation network using the gravity model. A theoretical framework is developed to explore the relationships between a region’s innovation capability and its network location. Hypotheses are tested using hierarchical regressions.FindingsFirst, this paper finds that a region’s network centrality can promote its innovation capability. Second, a structural hole can positively adjust the relationship between a region’s centrality and innovation capability. Third, a region’s indirect connections can inhibit its innovation capability while exhibiting a U-shaped relationship in moderating centrality and innovation capability.Originality/valueThis study uses a multi-index system to construct an innovation network covering 29 regions in China. This network represents the innovation cooperation and overall situation of innovation in China. The paper is one of the first attempts at investigating the relationship between regional network locations and innovation capability. It is also the first attempt at testing the influence of indirect connections on a region’s innovation capability. The findings provide a new perspective on the factors influencing regional innovation capacity and a new way for regions to improve their innovation capability.
How do consumers perceive old brands? Measurement and consequences of brand oldness associations
Authors: Huaman-Ramirez Richard , Maaninou Nada , Merunka Dwight , Cova Véronique 
Published: November 2020

Purpose
This paper aims to focus on brand oldness associations and their measurement. Research on brand age points to a variety of interpretations concerning perceived brand oldness and establishes the existence of multiple positive consequences such as brand credibility, trust or attachment. However, the complexity and measurement of brand oldness associations are not yet well-established. This paper proposes a reliable and valid measurement scale of the concept.

Design/methodology/approach
The research follows a rigorous scale-development procedure based on eight empirical studies, with a total of 2,065 respondents. The data were analyzed through covariance-based structural equation modeling.

Findings
The scale consists of 18 items and six dimensions, namely, decline, expertise, maintenance, reminiscence, timelessness and tradition. Results demonstrate an effect of brand oldness associations on both brand attachment and brand equity.

Research limitations/implications
The research was conducted in one country (France). Additional studies in other settings or countries should be carried out to establish generalizability of results and strengthen causality inferences.

Originality/value
This is the first research to study the notion of brand oldness associations. This study identifies its dimensions, develops a measurement scale and demonstrates its reliability and validity.
House prices and bank credit in China: analysis of first- and second-tier cities
Authors: Chin Lee , Li Xiaoran 
Published: November 2020

Purpose
Housing prices in China have increased rapidly over the past decade. Motivated by the fact that the real estate market and bank credit scale are vastly different in Chinese cities, the purpose of this paper is to compare the impact of bank credit on house prices in first- and second-tier cities in China.

Design/methodology/approach
In this study, a panel data method was used to investigate 19 first-tier cities and 30 second-tier cities between the period 2003 and 2018.

Findings
The empirical analysis undertaken in this study found that bank credit was relevant to house prices but varied in different cities in which house prices in second-tier cities tended to be more affected by bank credit compared to those in first-tier cities. In contrast, population was found to be a dominant factor that influenced house prices in first-tier cities. Likewise, the factors, per capita and gross domestic product, were found to exert a significant influence on house prices in first- and second-tier cities.

Practical implications
This paper provided numerous policies to control the price of housing in first- and second-tier cities.

Originality/value
The housing prices, bank credit scale and population distribution are vastly different in different cities in China. This research considers these differences while examining the dominant factors that affect house prices in first- and second-tier cities in China.
Should children become key stakeholders in designing family-friendly workplaces?
Authors: Krstić Nataša , Sladojević Matić Jelena 
Published: November 2020

PurposeThe purpose is to determine whether there is a gap between what children and youth expect from their parents' workplaces and the family-friendly business practices that employers apply, as well as whether COVID-19 has accelerated the introduction of these practices or contributed to any paradigm change.Design/methodology/approachDescriptive methodological research was done through electronic surveys with two target groups. The first group of respondents consisted of 1,279 children and youth who expressed their opinion on the impact of the parental workplace on them and what needs to be done to make companies more family oriented. The second survey involved 64 employers, who shared their views on achieving a balance between work and family and provided insight into the pre-pandemic and pandemic family-friendly workplace practices.FindingsAn apparent gap was identified between the family-friendly workplace practices offered by employers and the needs that children have regarding their parents' workplace. Although employers confirm that COVID-19 provides an opportunity to encourage FFW practices, during the outbreak of the virus, they demonstrated responsibility towards employees but neglected their family members.Research limitations/implicationsThe findings cannot be generalised to the entire business sector as the survey is not nationally representative. Also, the surveyed children do not come from the surveyed employers' work collectives, so it was not possible to intersect the findings.Practical implicationsThe research is vital for human resource managers as creators and implementers of family-friendly workplaces, as it indicates the need to involve the important but overlooked stakeholders in this process – the children of employees.Originality/valueThe research gap in the family-friendly workplace paradigm is addressed by comparing the attitudes of children with the employers' practices, before and during the COVID-19 outbreak.
Influence of work–family conflict on the emergence of nascent mumpreneurs community in Jordan
Authors: Abdelsalam Rasha , Al-Haddad Shafig , Zeqiri Jusuf 
Published: November 2020

Purpose
The purpose of this study is to find out the influence of work–family conflict on mumpreneurial intentions in Jordan, specifically among the community of nascent mumpreneurs.

Design/methodology/approach
A quantitative-cross sectional study was conducted where a convenience sampling was used to gather information from 81 nascent mumpreneurs. The data were collected through an online survey distributed to nascent mumpreneurs in Jordan.

Findings
The findings showed the mumpreneurs intentions of nascent mumpreneurs were influenced mainly by the work–family conflict on mumpreneurs intentions among nascent mumpreneurs.

Originality/value
It is recommended that more incubators should be established specifically for mumpreneurs and use the results of this study to better develop workshops and training to future and potential mumpreneurs.
Entrepreneurship in microfranchising: an emerging market perspective
Authors: Awuh Harrison Esam , Dekker Marleen 
Published: November 2020

Purpose
Building on the limitations of the efforts of aid agencies and non-governmental organisations to pull the poor out of poverty in low- and middle-income countries and declining opportunities for market expansion in high-income countries, microfranchising is being promoted as a pro-poor business model, which promotes entrepreneurship. Sub-Saharan Africa has become a fertile ground for the propagation of this model. However, contemporary studies on microfranchising have not sufficiently explored what motivates people to turn to this method of doing business.

Design/methodology/approach
Through the case of a microfranchise in Ghana (FanMilk), the purpose of this paper is to use qualitative methods to study motivations for engaging in entrepreneurship ventures in a microfranchise.

Findings
The findings reveal whether motivations for becoming microfranchise entrepreneurs change over time or are varied, and these changes are moderated by changing opportunities, challenges and demographic factors.

Originality/value
These findings contribute to knowledge on microfranchising in terms of theory, policy and practice. The findings also seek to stimulate further inquiry into microfranchising and its ability to create value for multiple parties when operating in emerging markets such as Africa.
Knowledge, attitudes and preventive behaviors toward COVID-19: a study among higher education students in Portugal
Authors: Alves Regina Ferreira , Samorinha Catarina , Precioso José 
Published: November 2020

PurposeThe purpose of this study was to assess knowledge, attitudes and behaviors about COVID-19 among Portuguese higher education students.Design/methodology/approachIn May 2020, all students from a Portuguese University were invited to participate in completing an online questionnaire. A total of 262 students participated. COVID-19 related knowledge, attitudes toward COVID-19 and preventive behaviors were assessed. Differences between outcomes and sociodemographics were analyzed through independent t-tests and the ANOVA. A generalized linear model was calculated to determine the predictive variables of preventive behaviors.FindingsStudents revealed good knowledge about COVID-19, correctly answering 13.06 (SD = 1.25) questions in a total of 14 and favorable attitudes toward preventive behaviors (M = 32.73, SD = 2.88). Students reported always engaging in, on average, 5.81 (SD = 2.61) of the 12 behavior analyzed. Females presented higher levels of knowledge, more positive attitudes and engaged in more preventive behaviors than males. Being a bachelor's (Exp (β) = 8.213, 95% CI: 1.791–37.670, p < 0.01) or a master's degree student (Exp (β) = 7.568, 95% CI: 1.598–35.835, p < 0.05) and having positive attitudes toward preventive behavior of COVID-19 predicted the adoption of those preventive behavior (Exp (β) = 1.340, 95% CI: 1.189–1.510, p < 0.001).Originality/valueThis study provides useful data to plan health education programs about COVID-19 among higher education students. The continuous investment by universities in preventive campaigns is essential to promote good preventive behaviors in the next academic year.
Role of impulsiveness in online purchase completion intentions: an empirical study among Indian customers
Authors: G. Rejikumar , Asokan-Ajitha Aswathy 
Published: November 2020

Purpose
Online cart abandonment is a severe issue posing challenges to e-commerce growth. Emerging economies such as India fascinates global marketing practitioners because of favorable demographics and high levels of internet penetration. This study aims to consider the role of certain exogenous factors in developing shopping motivations that sequentially mediate to online purchase completion through impulsiveness under risk perceptions. The primary motivation behind this study is to understand the mental mechanism among online customers that develop purchase completion intentions, which prevent cart abandonment significantly.

Design/methodology/approach
Impact of e-commerce exogenous factors related to e-commerce such as website attributes, product features, promotional excellence and decision-making easiness on shopping motivations, impulsiveness and purchase completions intentions under the moderating effect of risk was estimated from the perceptions of Indian online customers (n = 243) using variance-based structural equation modeling and SPSS process macro v.3.0.

Findings
The most important exogenous variable that can influence purchase completion directly, sequentially through shopping motivations is decision easiness and promotions. Even though utility motivations are dominant in purchase completion intentions, hedonistic aspects are more critical in developing impulsiveness. The translation of impulsiveness to purchase completion is happening, but risk perception significantly moderates impulsiveness formation.

Research limitations/implications
Theoretically, this study examined online purchase completions being the most sought response by a customer to various stimuli in e-commerce. The study adopted a moderated mediation analysis in which shopping motivations and impulsiveness were mediators and risk as moderator. The interaction effect of risk on purchase completions was significant even when the mediating effects were prominent.

Practical implications
Contributes to the current knowledge-related online buying behavior in virtual retail formats and helps marketers in streamlining their focus in using impulsiveness as a strategic tool in reducing cart abandonment.

Originality/value
This study helps in understanding emerging trends in online buying behavior in India.