bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treatment of Insertional Achilles Tendinopathy With Ultrasound-Guided Intrabursal Retrocalcaneal Hyaluronic Acid Injection - A Prospective Study
Authors: Slevin Omer , Segal David , Ohana Nissim , Kots Eugene , Feldman Viktor , Nyska Meir , Palmanovich Ezequiel 
Published: December 2020

Abstract
Background: Insertional Achilles tendinopathy (IAT) is a chronic degenerative enthesopathy involving fibrocartilage changes that resemble osteoarthritic changes in articular cartilage. Thus, our primary goal was to evaluate the effect of hyaluronic acid (HA) injections on IAT. Methods: Fifteen IAT ankles (14 patients) were treated with three consecutive weekly ultrasound-guided retrocalcaneal intrabursal injections of hylan G-F 20 (Synvisc®). Patients answered the "Victorian Institute of Sport Assessment – Achilles" (VISA-A) questionnaire before every injection and on 1 month and 6 months follow-up visits. Univariate analysis was performed to identify differences in functional scores. Results: The mean (VISA-A) score improved significantly following HA injections from 34.8 ± 15.2 (range, 11-63) points before the first injection to 53.6 ± 20.9 (range, 15-77) points after 1 month, and 50.7 ± 18.6 (20-75) points after 6 months. No adverse drug reactions were noted.Conclusions: Three consecutive ultrasound-guided intrabursal retrocalcaneal HA injections were found in our cohort to be beneficial in treating IAT.Trial registration: NCT02368561. Registered 23 February 2015. https://clinicaltrials.gov/ct2/show/NCT02368561?term=insertional+achilles&draw=2&rank=2
Metformin-induced TRAIL Upregulation Promotes Apoptosis in Triple Negative Breast Cancer and Non-small Cell Lung Cancer Cells
Authors: Liu Shuang , Polsdofer Erik V , Zhou Lukun , Ruan Sanbao , Lyu Hui , Hou Defu , Liu Hao , Thor Ann D , He Zhimin , Liu Bolin 
Published: December 2020

Abstract
Background: Triple negative breast cancer (TNBC) and non-small cell lung cancer (NSCLC) are highly aggressive types of cancer with limited therapeutic options. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) shows promising antitumor activity and is well tolerated in preclinical studies. However, the efficacy of recombinant TRAIL in clinical trials is compromised in part by its short serum half-life and low in vivo stability. Induction of endogenous TRAIL may overcome the limitations and become a new strategy for cancer treatment. Methods: Cell proliferation (MTS) and colony formation assays were performed to determine the anti-proliferative/anti-survival effects of metformin, a common drug for type II diabetes, on TNBC and NSCLC cells. A Live/Dead imaging assay and specific apoptotic ELISA analyzed cells undergoing apoptosis. Western blot analyses were used to examine protein expression and cleavage. A recombinant TRAIL-R2-Fc chimera protein was applied to block TRAIL binding to its receptors. Lentiviral vector containing shRNAs was used to specifically knockdown TRAIL expression. A tumor xenograft model was established by inoculation of H460 cells into nude mice. The tumor-bearing mice were treated with metformin to assess the drug’s antitumor activity. Immunohistochemistry was carried out to study the effects of metformin on tumor cell proliferation and induction of apoptosis and TRAIL in vivo. Results: Metformin upregulated TRAIL protein, but not mRNA expression, which correlated with increased apoptosis in TNBC and NSCLC cells. Metformin did not alter the expression of TRAIL receptors (TRAIL-R1/DR4 and TRAIL-R2/DR5). Metformin-induced TRAIL was secreted into conditioned medium (CM) and functional, since the CM potently promoted apoptosis in MDA-MB-231 cells, which was effectively blocked by a recombinant TRAIL-R2-Fc chimera protein. Inhibition of TRAIL function by blockade of its binding to DR4/DR5 or specific knockdown of TRAIL expression significantly attenuated metformin-induced apoptosis. Studies with a tumor xenograft model revealed that metformin not only significantly inhibited tumor growth; it also elicited apoptosis and upregulated TRAIL expression in vivo. Conclusions: TRAIL upregulation and activation of death receptor signaling are pivotal for metformin-induced apoptosis in TNBC and NSCLC cells. Our studies identify a novel mechanism of action of metformin exhibiting potent antitumor activity via induction of endogenous TRAIL.
Radiomic Feature Analysis for Distinguishing Solitary Pulmonary Capillary Hemangioma From Lepidic-Predominant Lung Adenocarcinoma in Ground Glass Lung Nodules
Authors: Lin Mong-Wei , Wang Hao-Jen , Chen Yi-Chang , Chen Li-Wei , Hsieh Min-Shu , Yang Shun-Mao , Chen Ho-Feng , Wang Chuan-Wei , Chen Jin-Shing , Chang Yeun-Chung , Chen Chun-Ming 
Published: December 2020

Abstract
Solitary pulmonary capillary hemangioma (SPCH) is a benign lung tumor that presents as ground-glass nodules (GGN) on computed tomography (CT) images, mimicking lepidic-predominant adenocarcinoma (LPA). This study aimed to establish a discriminant model using a radiomic feature analysis to distinguish SPCH from LPA in lung GGNs. This study included 13 and 49 patients who underwent complete resection for lung SPCH and LPA, respectively. An SPCH/LPA classification model was proposed based on a two-level decision tree and 26 radiomic features extracted from each segmented lesion, including 5 and 21 features from the histogram and co-occurrence matrix, respectively. The two-level decision tree was constructed based on the training data with a support vector machine (SVM) as the classifier in each tree node. For comparison, a baseline model was built with the same 26 features using an SVM as the classifier. Both models were assessed by the leave-one-out cross-validation method. The area under the receiver operating characteristic curve, accuracy, sensitivity, and specificity of the proposed SPCH/LPA model were 0.954, 91.9%, 92.3%, and 91.8%. The proposed SPCH/LPA model significantly outperformed the baseline model (p<0.05). Our results may help surgeons to preoperatively discriminate SPCH from LPA, thus avoiding unnecessary surgery for benign tumors.
Impact of Chronic Outward Force on Arterial Responses of Proximal and Distal of Long Superficial Femoral Artery Stent
Authors: Li Hu , Rha Seung-Woon , Choi Byoung Geol , Choi Se Yeon , Moon Sang Ki , Jang Won Young , Kim Woohyeun , Ahn Ji Hun , Park Sang-Ho , Choi Woong Gil , Yang Rui Feng , Bai Wen Wei , Choi Cheol Ung , Ryu Yang gi , Baek Man Jong , Oh Dong Joo 
Published: December 2020

Abstract
Background

Self-expanding nitinol stent (SENS) implantation is commonly oversized in the superficial femoral artery (SFA), and leads to chronic outward force (COF) and in-stent restenosis (ISR). This study aimed to investigate the impact of COF of oversizing SENS on ISR of SFA.
Methods

In patients with implanted SENS in SFA, intimal hyperplasia especially between proximal segment and distal segment was evaluated by quantitative angiography, and the impact of COF on mid-term angiographic outcomes was investigated. In addition, porcine model with implanted SENS was used to evaluate the impact of COF on angiographic and histopathologic outcomes at 1 month. Excised stented arteries were evaluated by histopathologic analysis.
Results

We analyzed 65 SENS in 61 patients with follow-up angiography at 6 months to 1 year. In the long SFA stent, stent-to-vessel ratio was significantly higher in the distal stent than in the proximal stent (1.3 ± 0.2 vs. 1.55 ± 0.25, P = 0.001). ISR incidence was higher at the distal stent (37.3% vs 52.6%, P = 0.029). After 4 weeks of SENS implantation in porcine model, restenosis % was 29.5 ± 12.9% versus 46.8 ± 21.5% (P = 0.016). The neointimal area was 5.37 ± 1.15 mm2 vs. 8.53 ± 5.18 mm2 (P = 0.05). The restenosis % was 39.34 ± 8.53% versus 63.97 ± 17.1% (P = 0.001).
Conclusions

The development of a tapered type stent for longer SFA lesion and the development of SENS with smaller diameter are necessary, particularly in Asians.
Cyanoacrylate Tissue Adhesive as a Membrane in Socket Preservation: In Vitro Cytotoxicity and Clinical Evaluation
Authors: Azpur Gerardo Mendoza , ortiz Junior , Valdivia Erick , Balbinot Gabriela de Souza , Collares Fabricio Mezzomo , Mauricio Jonathan Meza , Shibli Jamil A 
Published: December 2020

Abstract
Background: Cyanoacrylate-based adhesives provide immediate hemostasis when applied; this is attributed to their ability to form a mechanical barrier in the surgical site, favoring the coagulation process and allowing control of bleeding. The purpose of the present study was to demonstrate the in vitro effect of cyanoacrylate-based tissue adhesive and the clinical evaluation of wound healing of freshly extracted sockets with adhesives applied on an exposed collagen membrane, for preservation of the alveolar ridge. Methods: Cytotoxicity was evaluated with sulphoradamine B assay with primary fibroblasts from gingival tissue. The in vivo analysis was carried out in 10 patients needing extraction of single-rooted teeth, who met the inclusion and exclusion criteria and signed the term of free and informed consent. Alveolar preservation was carried out with a mineralized cortical allograft bone substitute and a resorbable collagen membrane that was intentionally left exposed. On the exposed membrane, the Periacryl® 90 HV was applied as a barrier. The biocompatibility of the adhesive was assessed by the Early-Wound Healing Index (EHI) and Early Wound Healing Score (EHS), of perialveolar tissues at time intervals of 12, 30- and 60-days post-surgery. Results: Reduced cell viability was observed for Periacryl® 90 HV when compared with cells without adhesive treatments. No postoperative complications were observed after the application of Periacryl® 90 HV on perialveolar tissues. Conclusion: The use of the Periacryl® 90 HV cyanoacrylate-based tissue adhesive resulted in reduced fibroblast viability in vitro, and adequate results of wound closure in the clinical evaluation of EHI and EHS scores.
Transient Feature Extraction method based on adaptive TQWT sparse optimization
Authors: liu xue , sun ao , hu jian 
Published: December 2020

Abstract
Aiming at the problem of strong impact, short response period and wide resonance frequency bandwidth of transient vibration signals, a transient feature extraction method based on adaptive Tunable Q-factor Wavelet Transform (TQWT) was proposed. Firstly, the characteristic frequency band of the vibration signal was selected according to the time-frequency distribution. Based on the characteristic frequency band, the sub-band average energy weighted wavelet Shannon entropy was used to optimize the number of decomposition layers, quality factor and redundancy of TQWT, so as to achieve the adaptive optimal matching of the impact characteristic components in the vibration signal. Then, according to the characteristics of the transient impact of the telemetry vibration signal, the TQWT decomposition coefficients were sparse reconstructed to obtain more sparse impact characteristics, and the weighted power spectrum kurtosis was used as the impact characteristic index to select the optimal sub-band, Finally, the inverse transform of TQWT was used to reconstruct the optimal sub-band to enhance its weak impact features. The simulation and measured signal processing results verify the effectiveness of the algorithm.
A Novel Patient-Derived Cell Line of Adrenocortical Carcinoma Shows a Pathogenic Role of Germline MUTYH&nbsp;Mutation and High Tumour Mutational Burden
Authors: Landwehr Laura-Sophie , Schreiner Jochen , Appenzeller Silke , Kircher Stefan , Herterich Sabine , Fassnacht Martin , Kroiss Matthias , Weigand Isabel 
Published: December 2020

Abstract
BackgroundThe response of advanced adrenocortical carcinoma (ACC) to current chemotherapies is unsatisfactory and a limited rate of response to immunotherapy was observed in clinical trials. High tumour mutational burden (TMB) and the presence of a specific DNA signature are characteristic features of tumours with mutations in the gene MUTYH encoding the mutY DNA glycosylase. Both have been shown to potentially predict the response to immunotherapy. High TMB in an ACC cell line model has not been reported yet.MethodsThe JIL-2266 cell line was established from a primary ACC tumour, comprehensively characterised and oxidative damage, caused by a dysfunctional mutY DNA glycosylase, confirmed.ResultsHere, we characterise the novel patient-derived ACC cell line JIL-2266, which is deficient in MUTYH-dependent DNA repair. JIL-2266 cells have a consistent STR marker profile that confirmed congruousness with primary ACC tumour. Cells proliferate with a doubling time of 41±13 hours. Immunohistochemistry revealed positivity for steroidogenic factor-1. Mass spectrometry did not demonstrate significant steroid hormone synthesis. JIL-2266 have hemizygous mutations in the tumour suppressor gene TP53 (c.859G>T:p.E287X) and MUTYH (c.316C>T:p.R106W). Exome sequencing showed 683 single nucleotide variants and 4 insertions/deletions. We found increased oxidative DNA damage in the cell line and the corresponding primary tumour caused by impaired mutY DNA glycosylase function and accumulation of 8-oxoguanine.ConclusionThis model will be valuable as a pre-clinical ACC cell model with high TMB and a tool to study oxidative DNA damage in the adrenal gland.
Bacterial morphotypes as biodiversity indicators in a scarcely explored ecosystem in Santiago de Cali, Colombia
Authors: Vergara-López Laura , Díaz-Ortíz Alejandro , Rosero-García Doris 
Published: December 2020

Abstract
Bacteria are considered as the most diverse microorganisms on earth. However, bacterial diversity is unknown in some ecosystems, such as leachate treatment plants. In the present study, we characterized culturable bacteria isolated from six lagoons and one pure leachate to assess whether bacterial morphotypes can be used as biodiversity indicators. The sampling site was a leachate treatment plant located in Santiago de Cali. Overall, 311 bacterial morphotypes were collected from six lagoons, and a pure landfill leachate, and were identified using colony characters. Four biodiversity indexes related to species richness and abundance as well as to the estimation of the number of species present were calculated. The results show a great diversity of bacterial morphotypes in all samples. Further, the highest estimates of biodiversity were observed at lagoon 6 physicochemically treated, and the lowest estimates were observed at lagoons 5 and 8. The leachate generated in the sampled lagoons, which were composed of different elements, allows for the colonization of diverse bacteria. Moreover, these bacteria were resistant to the treatment plant processes, thereby explaining the highest diversity observed at the lagoon 6. Our results showed that bacterial morphotypes can be used as biodiversity indicators in ecosystems.
Spiking-Free HGO-Based Dynamic Surface Control for Flexible Joint Manipulator with Unmeasurable States
Authors: Kong Dezhi , Wang Wendong , Shi Yikai 
Published: December 2020

Abstract
For the flexible joint manipulator control system (FJMCS) with unmeasurable states, a novel partial states feedback control (PSFC) is proposed. Firstly, the unmeasurable states and the uncertainties are observed by a high-gain observer (HGO) simultaneously. Then, a dynamic surface controller is proposed based on the output of the HGO. The newly proposed controller has several advantages over existing methods. First, the proposed controller not only uses the estimate states to avoid using unmeasurable states, but also uses the estimation of uncertainties to enhance the robustness of FJMCS. Second, a novel spike suppression function (SSF) is developed to avoid the estimation spike problem in the existing HGO-based controllers. The closed-loop system stability is proved by the Lyapunov theory. Simulation results demonstrate the effectiveness of the proposed controller.
Genome-Wide Identification, Expression and Potential Function Analysis of the ERF and DREB Subfamily Members in Tomato
Authors: Liu YuDong , Zhang Li , Chen LiJing , Pang ShengQun , Zheng Qun , Quan ShaoWen , Liu YuFeng , Xu Tao , Qi MingFang 
Published: December 2020

Abstract
Background: APETALA2/ethylene responsive factors (AP2/ERFs) are unique regulators found in the plant kingdom that are involved in all life activity processes, including flowering, fruit ripening, floral meristem growth, and defense responses. In tomato (Solanum lycopersicum), there are 60 DREB and 80 ERF subfamily members, however, their functionality remains poorly understood.Results: In this work, the AP2 domain conserved amino acid sequences of 68 ERF proteins from 20 plant species were compared and a Multiple Em for Motif Elicitation (MEME) analysis was conducted. Results revealed that the 9th amino acid of the AP2 domain exhibited marked characteristics during the selection of DRE/CRT and/or GCC boxes as protein binding sites. Moreover, motifs near the AP2 domain may be involved in protein binding to DNA, whereas motifs far away from the AP2 domain may function as a part of the transactivation domain. Furthermore, we compared the expression levels of all ERF genes in 30 tomato organs and under biotic and abiotic stresses. Results indicated that most of 17 ERF and DREB repressor genes were highly expressed in almost all tomato organs and under some biotic and abiotic stress. The transcripts per million (TPM) value ratios of all repressor genes exceeded that of all activator genes in 16 tomato organs. Thus, it can be inferred that these repressor genes play vital roles in balancing the regulatory functions of activator genes and activator genes may also conversely compete with repressor genes to ensure normal growth, development, and defense responses in tomato.Conclusions: This work uncovered the potential functions of all ERF and DREB genes that regulate tomato growth, development, and defense responses, and considers the binding ability of the AP2 domain unique sequences with DRE/CRT and GCC boxes, as well as the relationship of unique motifs with the transactivation domain. These findings will expand upon our understanding of the functions of ERF and DREB genes in tomato.
Identification of a hypoxia-associated long non-coding RNA signature and nomogram as a prognostic target for clear cell renal cell carcinoma
Authors: Chen Hualin , Pan Yang , Jin Xiaoxiang , Chen Gang 
Published: December 2020

Abstract
Background

To develop a hypoxia-associated long non-coding (lncRNA) signature for risk stratification in clear cell renal cell carcinoma (ccRCC).
Methods

The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma (TCGA KIRC) dataset was downloaded and analyzed.
Results

Four prognostic hypoxia-associated lncRNAs were used for signature construction. The four lncRNAs were downregulated in high grade, advanced stage, and high-risk ccRCC. ccRCC patients in the high-risk group had a worse prognosis than those in the low-risk group. And the risk score was significantly higher in high grade and advanced stage. The signature had an independent and long-standing prognosis prediction ability up to 10-year follow-up. Notably, the risk score was significantly positively correlated with the infiltration abundances of six immune cells from the Tumor IMmune Estimation Resource (TIMER). The gene set enrichment analysis (GSEA) also suggested that the signature was involved in the metabolism and tumorigenesis, which were closely related to the hypoxic tumor microenvironment. Ultimately, a nomogram was built to predict the individual long-term survival possibility.
Conclusions

We have developed a new hypoxia-associated lncRNA signature, representing a promising tool for risk stratification tool in ccRCC. It might serve as a prognostic index to facilitate personalized counseling for treatment.
Recovery of Olfactory and Gustatory Dysfunctions in COVID-19 Patients in Iran: a Prospective Cohort Study
Authors: Balajelini Mohammad Hosein Taziki , Vakili Mohammad Ali , Rajabi Abdolhalim , Mohammadi Masoud , Tabarraei Alijan , Hosseini Seyed Mehran 
Published: December 2020

Abstract
Background: Olfactory and gustatory dysfunctions (OD and GD) manifested unpredictably in COVID-19 patients. Considering the high prevalence of OD and GD and the importance of the sense of smell and taste in the quality of life, this study aimed to determine the association of some demographic and clinical factors with OD and GD recovery in COVID-19 patients in Iran.Methods: This prospective cohort study was performed on COVID-19 patients with OD and GD during the first and second peak of pandemic in Golestan province, Iran. We used a local health care registry system for data collection. All cases had positive RT-PCR test for SARS-CoV-19 in nose and nasopharynx swab samples. Variables included age, sex, ethnicity, geographical location, time of onset of symptoms, severity of OD and GD (complete or partial). The follow-up time for recovery from OD and GD was six months. Time to recovery for OD and GD was estimated by Kaplan-Meier considering complete recovery as an endpoint. Differences among groups were evaluated using log-rank test and Cox proportional-hazard models as appropriate in SPSS version 16.Results: A total of 242 patients completed the study. The mean age of patients was 39.12±11.89 years. There were 127 (52%) females. After six months, 239 (98.8%) patients had complete OD recovery and 80.9% and 83.56% of patients recovered within the first 30 days following the onset of OD or GD, respectively. The mean time of OD recovery was significantly higher (35.07±4.25 days) in cases infected in the first peak of pandemic than in the second peak (21.65±2.05 days) (p=0.004). This finding also was observed for the recovery time of GD (p=0.005). The mean recovery time of GD was significantly higher in women than men (35.55±4.33 vs. 23.20±2.46 days) (p=0.04). The age (less than 20 years), second peak of epidemic, and partial dysfunction were identified as significant predictors for recovery.Conclusions: COVID-19–related OD and GD had high rate of recovery in the first month from onset of symptoms. Age of patients, severity of OD/GD and infecting in second peak of epidemic might be related to the recovery of OD or GD in patients with COVID-19.