bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-Term Progress and Behavior Complexification in Competitive Coevolution
Authors: Simione Luca , Nolfi Stefano 
Published: December 2020

Abstract
The possibility of using competitive evolutionary algorithms to generate long-term progress is normally prevented by the convergence on limit cycle dynamics in which the evolving agents keep progressing against their current competitors by periodically rediscovering solutions adopted previously. This leads to local but not to global progress (i.e., progress against all possible competitors). We propose a new competitive algorithm that produces long-term global progress by identifying and filtering out opportunistic variations, that is, variations leading to progress against current competitors and retrogression against other competitors. The efficacy of the method is validated on the coevolution of predator and prey robots, a classic problem that has been used in related researches. The accumulation of global progress over many generations leads to effective solutions that involve the production of articulated behaviors. The complexity of the behavior displayed by the evolving robots increases across generations, although progress in performance is not always accompanied by behavior complexification.
Wind- and Wave-Driven Reynolds Stress and Velocity Shear in the Upper Ocean for Idealized Misaligned Wind-Wave Conditions
Authors: Wang Dong , Kukulka Tobias 
Published: December 2020

AbstractThis study investigates the dynamics of velocity shear and Reynolds stress in the ocean surface boundary layer for idealized misaligned wind and wave fields using a large-eddy simulation (LES) model based on the Craik–Leibovich equations, which captures Langmuir turbulence (LT). To focus on the role of LT, the LES experiments omit the Coriolis force, which obscures a stress–current-relation analysis. Furthermore, a vertically uniform body force is imposed so that the volume-averaged Eulerian flow does not accelerate but is steady. All simulations are first spun-up without wind-wave misalignment to reach a fully developed stationary turbulent state. Then, a crosswind Stokes drift profile is abruptly imposed, which drives crosswind stresses and associated crosswind currents without generating volume-averaged crosswind currents. The flow evolves to a new stationary state, in which the crosswind Reynolds stress vanishes while the crosswind Eulerian shear and Stokes drift shear are still present, yielding a misalignment between Reynolds stress and Lagrangian shear (sum of Eulerian current and Stokes drift). A Reynolds stress budgets analysis reveals a balance between stress production and velocity–pressure gradient terms (VPG) that encloses crosswind Eulerian shear, demonstrating a complex relation between shear and stress. In addition, the misalignment between Reynolds stress and Eulerian shear generates a horizontal turbulent momentum flux (due to correlations of along-wind and crosswind turbulent velocities) that can be important in producing Reynolds stress (due to correlations of horizontal and vertical turbulent velocities). Thus, details of the Reynolds stress production by Eulerian and Stokes drift shear may be critical for driving upper-ocean currents and for accurate turbulence parameterizations in misaligned wind-wave conditions.
An Evaluation of the Utility of a Decision-Maker-Driven Climate Hazard Assessment Tool
Authors: Riley Rachel E. 
Published: December 2020

AbstractDecision-makers who have little to no formal training in atmospheric science are increasingly accessing and interpreting climate data and information within planning contexts. Many climate decision support tools (DSTs) have been developed to support decision-making across a variety of sectors and scales, but evaluation of such tools has only recently begun to take place. This study conducted a summative evaluation of the utility of a decision-maker-driven climate hazard assessment tool, the Simple Planning Tool (SPT), a climate DST. The SPT was inspired by and codeveloped with emergency managers, planners, and a boundary organization in two south-central U.S. states. The SPT’s target audience was surveyed to assess the tool’s utility, including its saliency, credibility, trustworthiness, and reasons for and impact of information use on decision-making. A high utility was found despite a relatively limited user base at the time of the study. In addition, SPT users represented a range of jurisdictional sizes, geographical scales, and years of experience. Although the small user sample limits generalizability of the study, it is likely a realistic reflection of the number of emergency managers and planners in the two states who are actively and regularly incorporating climate hazards into planning. The data also indicate that climate boundary organizations and climate service providers should work toward utilizing trusted information sources, channels, and procedures within the sectors to which their tool applies to help increase decision-maker awareness and use of their tool.
Occupational risk factors for shoulder chronic tendinous pathology in the Spanish automotive manufacturing sector: a case-control study
Authors: Rodriguez Diez-Caballero Beatriz , Alfonso-Beltrán Joaquín , Bautista Iker J. , Barrios Carlos 
Published: December 2020

Abstract
Background
Musculoskeletal Diseases (MSDs) are among the most prevalent health problems encountered in the workforce in Europe. Multiple risk factors contribute to their onset. In the present study, different individual risk factors for chronic tendinous pathology affecting the shoulder were analysed in a sample of workers from the automotive manufacturing sector.

Methods
An observational retrospective study was conducted with 73 cases of officially recognised and compensated occupational diseases and 94 aleatory cases of healthy workers from the same car assembly company. The experimental group comprised individuals with tendinous chronic pathology of the rotator cuff. Multiple variables that identified the risks present in the job were assessed along with participants clinical evaluation. Furthermore, two standardised guidelines for risk factors assessment were also used: the Spanish National Institute of Social Security (INSS) and the American Occupational Information Network (O*Net). Both descriptive statistical analysis and Odds ratios calculations considering the occupational disease as a dependent variable were performed.

Results
The use of hand tools, exposure to mechanical pressure in the upper limbs and awkward postures were the most prevalent risk factors. Pressure on the palm of the hand and the hand tool impacting the hand were also important risk factors. Some psychosocial factors such as lack of autonomy and mental workload were also associated shoulder tendinous diseases. The association of age, load handling, and awkward postures were the core risk factors responsible for most of the tendinous chronic injuries of the shoulder in this sample of car assembly workers.

Conclusions
Both ergonomic and psychosocial factors were present and increased the risk of developing occupational chronic tendinopathies at the shoulder in this sample of workers. Aging, load handling, and awkward postures showed the strongest predictive values. Greater knowledge of how risk factors interact would facilitate the design of better preventive workplace strategies.
Cutaneous Rosai – Dorfman disease in a patient with late syphilis and cervical cancer – case report and a review of literature
Authors: Bielach – Bazyluk Angelika , Serwin Agnieszka B. , Pilaszewicz – Puza Agata , Flisiak Iwona 
Published: December 2020

Abstract
Background
Cutaneous Rosai – Dorfman disease (CRDD) is extremely rare variant of idiopathic histiocytic proliferative disorder, which may manifest as a non-specific macules, papules, plaques or nodules ranging in size and colour from yellow – red to red -brown.

Case presentation
A 52-year-old female presented with three gradually enlarging, reddish - brown nodules on the right upper extremity lasting six months. The patients denied fever, weight loss, malaise. Clinical examination and imaging tests showed no sign of lymphadenopathy. A biopsy specimen of a nodule showed a dense dermal polymorphic infiltrate with numerous histiocytes exhibiting emperipolesis phenomenon. Immunohistochemical staining of the histiocytes showed S-100 protein (+), CD68(+), but CD1a (-). Aforementioned findings were consistent with CRDD characteristics. Additionally, a routine serological screening and confirmatory serological tests for syphilis were positive. Syphilis of unknown duration was diagnosed. The IgG antibodies titre against Chlamydia trachomatis was elevated. An isolated sensory impairment over the right trigeminal nerve was found on neurological consultation. Comprehensive gynaecological assessment was carried out because of patient’s complaints of bleeding after sexual intercourse and led to diagnosis of cervical cancer. The initial therapy with methotrexate was discontinued after three months due to neutropenia. Further therapy with dapson was ineffective, therefore complete surgical excision was recommended.

Conclusions
CRDD is a rare, benign condition especially difficult to diagnose due to lack of general symptoms and lymphadenopathy. Histopathologic examination with immunohistochemical staining, exhibiting characteristic and reproducible findings play a key role in establishing an accurate diagnosis. In the presented case activated histiocytes demonstrated in a lesional skin might be a response to immune dysregulation related to chronic, untreated sexually transmitted infections and cancer.
Effect of acupuncture for methadone maintenance treatment patients: study protocol of a randomized clinical trial
Authors: Wen Hao , Xu Shichao , Zeng Jingchun , Ge Shuqi , Liao Yuan , Tang Chunzhi , Xiao Songhua , Lu Liming 
Published: December 2020

Abstract
Background
Opioid dependence is an increasing public health problem all over the world. Patients with opioid dependence have to receive methadone maintenance therapy (MMT) as replacement therapy for years or even for their entire life. Acupuncture as a kind of therapy has been used to treat substance dependence for many years. Jin’s three-needle acupuncture (JTN), a type of acupuncture technique, has been applied to treat various diseases for several decades. However, JTN as an acupuncture technique has not been used to treat patients receiving MMT. Therefore, we designed a randomized controlled trial to evaluate the efficacy and safety of acupuncture as adjunctive therapy for patients receiving MMT.

Methods/design
This study is a parallel-arm, randomized controlled trial that aims to evaluate the efficacy and safety of acupuncture as adjunctive therapy for patients receiving MMT. A total of 140 eligible participants who range in age from 18 to 60 years and fulfil the Diagnostic and Statistical Manual of Mental Disorders, 5th edition (DSM-V), for opiate dependence will be enrolled into this study. All eligible participants will be randomly assigned to the acupuncture group or routine group in a 1:1 allocation ratio. Participants who are enrolled in the acupuncture group will receive MMT and JTN treatment for 30 min per session. Meanwhile, those who are assigned to the routine arm will receive MMT only. All 18 sessions of JTN treatment will be delivered over 6 weeks (3 per week) and followed by a 4-week follow-up period. The primary outcome measure will be the visual analogue scale (VAS) for drug craving and the daily consumption of methadone (DCOM). Secondary outcome measures will include the urine test for opioid use, the 36-item Short Form Survey (SF-36), the Beck Anxiety Inventory (BAI), the Beck Depression Inventory II (BDI-II) and Pittsburgh sleep quality index (PSQI). VAS, DCOM, BAI, BDI-II and the urine test for opioid use will be evaluated at baseline, the second week, the fourth week, the sixth week and the tenth week. SF-36 and PSQI will be assessed at baseline, the fourth week, the sixth week and the tenth week.

Discussion
The results of this trial will provide evidence on the efficacy and safety of acupuncture as adjunctive therapy for patients receiving MMT.

Trial registration
Chinese Clinical Trial Registry ChiCTR1900026357. Registered on 2 October 2019.
Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method
Authors: Prakas Petras , Strazdaitė-Žielienė Živilė , Januškevičius Vytautas , Chiesa Francesco , Baranauskaitė Agnė , Rudaitytė-Lukošienė Eglė , Servienė Elena , Petkevičius Saulius , Butkauskas Dalius 
Published: December 2020

Abstract
Background
Six Sarcocystis species are known to use cattle (Bos taurus) as the intermediate host, two of which, S. hominis and S. heydorni, are zoonotic. There is a need for a method that will enable rapid identification of the Sarcocystis species in cattle.

Methods
The diaphragm muscles of 102 cattle from Lithuania were examined for the presence of Sarcocystis spp., using two different methods for species identification. Individual sarcocysts were isolated from squash preparations of the diaphragm muscle under the light microscope, followed by genetic characterisation of excised cysts using sequence analysis of the 18S rRNA (18S rRNA) and cytochrome c oxidase subunit I (cox1) genes. The same cattle muscle samples were digested and species-specific PCR analyses targeting cox1 were developed to identify the Sarcocystis isolates to the species level.

Results
Under the light microscope, sarcocysts were detected in 87.3% of animals, and Sarcocystis infection was verified in all digested samples. Three species, namely S. cruzi (n = 20), S. bovifelis (n = 23) and S. hirsuta (n = 6), were identified by DNA sequence analysis of isolated sarcocysts. Based on sequence analysis of cox1, the level of genetic variability depended on Sarcocystis species and geographical location. Four Sarcocystis species, S. cruzi (96.1%), S. bovifelis (71.6%), S. hirsuta (30.4%) and S. hominis (13.7%), were confirmed in the digested samples. In individual samples, the most common finding was two species of Sarcocystis (44.1%), followed by three species (26.5%), a single species (24.5%) and four species (4.9%).

Conclusions
Although examination of tissue preparations under the light microscrope did not detect any sarcocysts belonging to S. hominis, this species was identified in the digested samples subjected to a cox1-specific PCR analysis. These results demonstrate the need for effective molecular diagnosis techniques to detect Sarcocystis spp., which may be present at a lower prevalence and not detectable among the limited number of sarcocysts identified individually under the light microscope.

Graphical Abstract
Transcriptome-wide profiles of circular RNA and RNA-binding protein interactions reveal effects on circular RNA biogenesis and cancer pathway expression
Authors: Okholm Trine Line Hauge , Sathe Shashank , Park Samuel S. , Kamstrup Andreas Bjerregaard , Rasmussen Asta Mannstaedt , Shankar Archana , Chua Zong Ming , Fristrup Niels , Nielsen Morten Muhlig , Vang Søren , Dyrskjøt Lars , Aigner Stefan , Damgaard Christian Kroun , Yeo Gene W. , Pedersen Jakob Skou 
Published: December 2020

Abstract
Background
Circular RNAs (circRNAs) are stable, often highly expressed RNA transcripts with potential to modulate other regulatory RNAs. A few circRNAs have been shown to bind RNA-binding proteins (RBPs); however, little is known about the prevalence and distribution of these interactions in different biological contexts.

Methods
We conduct an extensive screen of circRNA-RBP interactions in the ENCODE cell lines HepG2 and K562. We profile circRNAs in deep-sequenced total RNA samples and analyze circRNA-RBP interactions using a large set of eCLIP data with binding sites of 150 RBPs. We validate interactions for select circRNAs and RBPs by performing RNA immunoprecipitation and functionally characterize our most interesting candidates by conducting knockdown studies followed by RNA-Seq.

Results
We generate a comprehensive catalog of circRNA-RBP interactions in HepG2 and K562 cells. We show that KHSRP binding sites are enriched in flanking introns of circRNAs and that KHSRP depletion affects circRNA biogenesis. We identify circRNAs that are highly covered by RBP binding sites and experimentally validate individual circRNA-RBP interactions. We show that circCDYL, a highly expressed circRNA with clinical and functional implications in bladder cancer, is almost completely covered with GRWD1 binding sites in HepG2 cells, and that circCDYL depletion counteracts the effect of GRWD1 depletion. Furthermore, we confirm interactions between circCDYL and RBPs in bladder cancer cells and demonstrate that circCDYL depletion affects hallmarks of cancer and perturbs the expression of key cancer genes, e.g., TP53. Finally, we show that elevated levels of circCDYL are associated with overall survival of bladder cancer patients.

Conclusions
Our study demonstrates transcriptome-wide and cell-type-specific circRNA-RBP interactions that could play important regulatory roles in tumorigenesis.
Association of dietary behaviors, biochemical, and lifestyle factors with metabolic phenotypes of obesity in children and adolescents
Authors: Qorbani Mostafa , Khashayar Pouria , Rastad Hadith , Ejtahed Hanieh-Sadat , Shahrestanaki Ehsan , Seif Ehsan , Daniali Seyede Shahrbanoo , Goudarzi Masoomeh , Motlagh Mohammad Esmaeil , Khodaparast Zeinab , Heshmat Ramin , Kelishadi Roya 
Published: December 2020

Abstract
Background and aims
To examine the association of dietary behaviors, lifestyle, and biochemical factors with metabolic phenotypes of obesity among obese Iranian children and adolescents.

Methods
This cross-sectional study was conducted within the framework of the fifth phase of CASPIAN study. Of 3840 students aged 7–18 years of 30 Iranian provinces, 408 subjects were diagnosed as obese; they were divided into metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO) groups. Biochemical factors, anthropometric measures, dietary, and lifestyle habits were compared between groups.

Results
Of the 408 obese subjects, 68 (16.7%) were the MUO; the remaining 340 (84.3%) fall in the MHO group. The MUO group had significantly higher systolic and diastolic BPs, FBS, TG, ALT, anthropometric measures, and lower HDL levels than MHO groups (all p-value < 0.05). The frequency of high birth weight (> 4000 gr) was significantly higher in the MUO group than the MHO group (p-value: 0.04). A higher percentage of individuals with breastfeeding duration ≥ 6 month was found in the MUO group (95.5% (95% CI 86.1–98.6%)) compared to MHO group (85.7% (95% CI 80.4–89.7%)) (p-value = 0.04). Among dietary and lifestyle-related behaviors, only the frequency of salty snack consumption and eating food according to the parents’ request was significantly higher in the MUO group than the MHO group (p-value < 0.05).

Conclusion
Dietary habits and lifestyle factors may determine the obesity phenotypes in children and adolescents.
Supportive care: men’s expectations who undergoing in vitro fertilization treatment
Authors: Bayrami Roghieh , Janghorban Roksana , Effati-Daryani Fatemeh , Hajshafiha Masoumeh 
Published: December 2020

Abstract
Objective
Infertile couples perceive some needs and expectations during treatment. Most studies have focused on infertile women’s needs. The study was done to explore the expectations of men who undergoing in in vitro fertilization (IVF) treatment. Participants were men whose wives undergo IVF treatment in the IVF clinic of Shahid Motahhri hospital in Urmia. Purposive sampling was performed and data collection was done through in-depth semi-structured interviews until data saturation.

Results
“Supportive care” was emerged as the main theme consisted of two categories. The first category was “to be treated with attention in healthcare setting” which included three subcategories: Receiving couple based care, establishing counseling centers, and continuous care. The second category was “to be welcomed in the society” which included two subcategories: Financial support from community and close family, and changing community views about IVF treatment. Expectations of men undergoing IVF focuses on supportive care. They seek to receive the kind of care from health care setting and society. A deep understanding of the needs could help practitioners to consider men’s expectations, and assist policy makers and researchers to design and implement interventions and programs in infertility clinics which enhance the partnership of infertile men and fulfill their expectations.
Are single-lumen 5Fr and triple-lumen 6Fr PICCs suitable for hemodynamic assessment by trans-pulmonary thermodilution? A pilot study
Authors: D’Arrigo Sonia , Sandroni Claudio , Cacciola Sofia , Dell’Anna Antonio Maria , Pittiruti Mauro , Annetta Maria Giuseppina , Colosimo Cesare , Antonelli Massimo 
Published: December 2020

Abstract
Background
Single-lumen 4Fr or double-lumen 5Fr power injectable peripherally inserted central catheters (PICCs) are not accurate for trans-pulmonary thermodilution (TPTD), since they overestimate cardiac index and other TPTD-derived parameters when compared with centrally inserted central catheters (CICCs) because of the smaller size of their lumen. We hypothesize that PICCs with larger lumen size may be reliable for the cardiac index assessment using the TPTD.

Methods
This is a single-centre, prospective method–comparison study that included adult patients admitted in ICU who required a calibrated Pulse Contour hemodynamic monitoring system (VolumeView/EV1000™) for circulatory shock and had both PICC and CICC in place. We compared TPTD measurements via single-lumen 5Fr or triple-lumen 6Fr polyurethane power injectable PICCs with triple-lumen 7Fr CICC (reference standard). To rule out biases related to manual injection, measurements were repeated using an automated rapid injection system. We performed Bland–Altman analysis accounting for multiple observations per patient.

Results
A total of 320 measurements were performed in 15 patients. During the manual phase, the cardiac index measured with either single-lumen 5Fr or triple-lumen 6Fr PICCs were comparable with cardiac index measured with triple-lumen 7Fr CICC (3.2 ± 1.04 vs. 3.2 ± 1.06 L/min/m2, bias 2.2% and 3.3 ± 0.8 vs. 3.0 ± 0.7 L/min/m2, bias 8.5%, respectively). During the automated phase, triple-lumen 6Fr PICC slightly overestimated the cardiac index when compared to triple-lumen 7Fr CICC (CI 3.4 ± 0.7 vs. 3.0 ± 0.7 L/min/m2, bias 12.5%; p = 0.012). For both single-lumen 5Fr and triple-lumen 6Fr PICCs, percentage error vs. triple-lumen 7Fr CICC was below 20% (14.7% and 19% during the manual phase and 14.4% and 13.8% during the automated phase, respectively). Similar results were observed for TPTD-derived parameters.

Conclusions
During hemodynamic monitoring with TPTD, both single-lumen 5Fr PICCs and triple-lumen 6Fr PICCs can be used for cold fluid bolus injection as an alternative to CICC (ClinicalTrials.gov NCT04241926).