bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones
Authors: Malešević Milka , Di Lorenzo Flaviana , Filipić Brankica , Stanisavljević Nemanja , Novović Katarina , Senerovic Lidija , Polović Natalija , Molinaro Antonio , Kojić Milan , Jovčić Branko 
Published: November 2019

AbstractPseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C18-HSL). Commercial C18-HSL (20 µM) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.
Ultrawide Bandwidth Electromagnetic Wave Absorbers Using a High-capacitive Folded Spiral Frequency Selective Surface in a Multilayer Structure
Authors: Liu Tian , Kim Sung-Soo 
Published: November 2019

AbstractA high-capacitive frequency selective surface (FSS) with a new structure of folded spiral conductors is proposed as the small-array periodicity and low-frequency resonance FSS for ultra-wide bandwidth absorbers in a multilayer structure. Due to the folded structure with long effective segments and a small gap, a large value of capacitance for the lowest resonating frequency is obtained. Through a combination of the high-capacitive spiral FSS with other conventional FSSs (square loop, square patch) with a medium- and high-frequency resonance, an ultra-wide absorption bandwidth (4.7–50.0 GHz for −10 dB reflection loss) is designed with a small total thickness of 7.0 mm, which is close to the theoretical limit (6.7 mm). Admittance analysis is conducted for better insight into the optimization procedure. The free space measurement with a test sample prepared by the screen printing method also demonstrates a wide-bandwidth absorption result (5.2–44.0 GHz for −10 dB reflection loss, total thickness = 6.5 mm), which is in good agreement with the simulation result. In addition, the angular stability of the proposed wide-bandwidth absorber is discussed for both TE and TM polarizations in association with unit cell periodicity and grating lobes.
Presynaptic dysregulation of the paraventricular thalamic nucleus causes depression-like behavior
Authors: Kato Tomoaki M. , Fujimori-Tonou Noriko , Mizukami Hiroaki , Ozawa Keiya , Fujisawa Shigeyoshi , Kato Tadafumi 
Published: November 2019

AbstractThe paraventricular thalamic nucleus (PVT) is a part of epithalamus and sends outputs to emotion-related brain areas such as the medial prefrontal cortex, nucleus accumbens, and amygdala. Various functional roles of the PVT in emotion-related behaviors are drawing attention. Here, we investigated the effect of manipulation of PVT neurons on the firing patterns of medial prefrontal cortical (mPFC) neurons and depression-like behavior. Extracellular single-unit recordings revealed that acute activation of PVT neurons by hM3Dq, an activation type of designer receptors exclusively activated by designer drugs (DREADDs), and administration of clozapine N-oxide (CNO) caused firing rate changes in mPFC neurons. Moreover, chronic presynaptic inhibition in PVT neurons by tetanus toxin (TeTX) increased the proportion of interneurons among firing neurons in mPFC and shortened the immobility time in the forced swimming test, whereas long-term activation of PVT neurons by hM3Dq caused recurrent hypoactivity episodes. These findings suggest that PVT neurons regulate the excitation/inhibition balance in the mPFC and mood stability.
Mechanical vibration does not systematically reduce the tremor in essential tremor patients
Authors: Lora-Millán Julio Salvador , López-Blanco Roberto , Gallego Juan Álvaro , Méndez-Guerrero Antonio , González de la Aleja Jesús , Rocon Eduardo 
Published: November 2019

AbstractEssential tremor (ET) is a major cause of disability and is not effectively managed in half of the patients. We investigated whether mechanical vibration could reduce tremor in ET by selectively recruiting afferent pathways. We used piezoelectric actuators to deliver vibratory stimuli to the hand and forearm during long trials (4 min), while we monitored the tremor using inertial sensors. We analyzed the effect of four stimulation strategies, including different constant and variable vibration frequencies, in 18 ET patients. Although there was not a clear homogeneous response to vibration across patients and strategies, in most cases (50–72%) mechanical vibration was associated with an increase in the amplitude of their tremor. In contrast, the tremor was reduced in 5–22% of the patients, depending on the strategy. However, these results are hard to interpret given the intrinsic variability of the tremor: during equally long trials without vibration, the tremor changed significantly in 67% of the patients (increased in 45%; decreased in 22%). We conclude that mechanical vibration of the limb does not have a systematic effect on tremor in ET. Moreover, the observed intrinsic variability of the tremor should be taken into account when designing future experiments to assess tremor in ET and how it responds to any intervention.
Cytokine gene expression assay as a diagnostic tool for detection of Mycobacterium bovis infection in warthogs (Phacochoerus africanus)
Authors: Roos Eduard O. , Scott Leeré A. , Ndou Sedzani , Olea-Popelka Francisco , Buss Peter E. , de Klerk-Lorist Lin-Mari , Warren Robin M. , van Helden Paul D. , Sylvester Tashnica T. , Miller Michele A. , Parsons Sven D. C. 
Published: November 2019

AbstractMycobacterium bovis infection has been described in many wildlife species across Africa. However, diagnostic tests are lacking for many of these, including warthogs (Phacochoerus africanus). Most literature on suids has focused on using serological tools, with few studies investigating the use of cell-mediated immune response (CMI) assays. A recent study showed that warthogs develop measurable CMI responses, which suggests that cytokine gene expression assays (GEAs) may be valuable for detecting M. bovis-infection, as shown in numerous African wildlife species. Therefore, the aim of the study was to develop GEAs capable of distinguishing between M. bovis-infected and uninfected warthogs. Whole blood was stimulated using the QuantiFERON-TB Gold (In-Tube) system, using ESAT-6 and CFP-10 peptides, before determining the relative gene expression of five reference (B2M, H3F3A, LDHA, PPIA and YWHAZ) and five target (CXCL9, CXCL10, CXCL11, IFNG and TNFA) genes through qPCR. The reference gene H3F3A was the most stably expressed, while all target genes were significantly upregulated in M. bovis-infected warthogs with the greatest upregulation observed for CXCL10. Consequently, the CXCL10 GEA shows promise as an ante-mortem diagnostic tool for the detection of M. bovis-infected warthogs.
Therapeutic potential of N-acetylcysteine in acrylamide acute neurotoxicity in adult zebrafish
Authors: Faria Melissa , Prats Eva , Gómez-Canela Cristian , Hsu Chuan-Yu , Arick Mark A. , Bedrossiantz Juliette , Orozco Manuel , Garcia-Reyero Natàlia , Ziv Tamar , Ben-Lulu Shani , Admon Arie , Gómez-Oliván Leobardo Manuel , Raldúa Demetrio 
Published: November 2019

AbstractTwo essential key events in acrylamide (ACR) acute neurotoxicity are the formation of adducts with nucleophilic sulfhydryl groups on cysteine residues of selected proteins in the synaptic terminals and the depletion of the glutathione (GSx) stores in neural tissue. The use of N-acetylcysteine (NAC) has been recently proposed as a potential antidote against ACR neurotoxicity, as this chemical is not only a well-known precursor of the reduced form of glutathione (GSH), but also is an scavenger of soft electrophiles such as ACR. In this study, the suitability of 0.3 and 0.75 mM NAC to protect against the neurotoxic effect of 0.75 mM ACR has been tested in vivo in adult zebrafish. NAC provided only a mild to negligible protection against the changes induced by ACR in the motor function, behavior, transcriptome and proteome. The permeability of NAC to cross blood-brain barrier (BBB) was assessed, as well as the ACR-scavenging activity and the gamma-glutamyl-cysteine ligase (γ-GCL) and acylase I activities. The results show that ACR not only depletes GSx levels but also inhibits it synthesis from NAC/cysteine, having a dramatic effect over the glutathione system. Moreover, results indicate a very low NAC uptake to the brain, probably by a combination of low BBB permeability and high deacylation of NAC during the intestinal absorption. These results strongly suggest that the use of NAC is not indicated in ACR acute neurotoxicity treatment.
Male flowers of Aconitum compensate for toxic pollen with increased floral signals and rewards for pollinators
Authors: Jacquemart A.-L. , Buyens C. , Hérent M.-F. , Quetin-Leclercq J. , Lognay G. , Hance T. , Quinet M. 
Published: November 2019

AbstractMany plants require animal pollinators for successful reproduction; these plants provide pollinator resources in pollen and nectar (rewards) and attract pollinators by specific cues (signals). In a seeming contradiction, some plants produce toxins such as alkaloids in their pollen and nectar, protecting their resources from ineffective pollinators. We investigated signals and rewards in the toxic, protandrous bee-pollinated plantAconitum napellus, hypothesizing that male-phase flower reproductive success is pollinator-limited, which should favour higher levels of signals (odours) and rewards (nectar and pollen) compared with female-phase flowers. Furthermore, we expected insect visitors to forage only for nectar, due to the toxicity of pollen. We demonstrated that male-phase flowers emitted more volatile molecules and produced higher volumes of nectar than female-phase flowers. Alkaloids in pollen functioned as chemical defences, and were more diverse and more concentrated compared to the alkaloids in nectar. Visitors actively collected little pollen for larval food but consumed more of the less-toxic nectar. Toxic pollen remaining on the bee bodies promoted pollen transfer efficiency, facilitating pollination.
-196 to -174del, rs4696480, rs3804099 polymorphisms of Toll-like receptor 2 gene impact the susceptibility of cancers: evidence from 37053 subjects
Authors: Gao Sheng-Lin , Chen Yi-Ding , Yue Chuang , Chen Jiasheng , Zhang Li-Feng , Wang Si-Min , Zuo Li 
Published: November 2019

Abstract
Relationship between Toll-like receptor-2 (TLR2) and cancer risk has been illustrated in some studies, but their conclusions are inconsistent. Therefore, we designed this meta-analysis to explore a more accurate conclusion of whether TLR2 affects cancer risks. Articles were retrieved from various literature databases according to the criteria. We used STATA to calculate the odds ratio (OR) and 95% confidence interval (95% CI) to evaluate the relationship between certain polymorphism of TLR2 and cancer risk. Finally, 47 case–control studies met the criteria, comprising 15851 cases and 21182 controls. In the overall analysis, people are more likely to get cancer because of -196 to -174del in TLR2 in all five genetic models, B vs. A (OR = 1.468, 95% Cl = 1.129–1.91, P=0.005); BB vs. AA (OR = 1.716, 95% Cl = 1.178–2.5, P=0.005); BA vs. AA (OR = 1.408, 95% Cl = 1.092–1.816, P=0.008); BB+BA vs. AA (OR = 1.449, 95% Cl = 1.107–1.897, P=0.007); BB vs. BA+AA (OR = 1.517, 95% Cl = 1.092–2.107, P=0.013). Meanwhile, rs4696480 could significantly increase the risk of cancer in Caucasians, furthermore, rs3804099 significantly decreased cancer risk in overall analysis, but more subjects are necessary to confirm the results. All in all, this meta-analysis revealed that not only -196 to -174del increased the risk of among overall cancers, Caucasians are more likely to get cancer because of rs4696480, while rs3804099 polymorphism could reduce the risk of cancer in some genetic models. There is no direct evidence showing that rs5743708, rs3804100 and rs1898830 are related to cancer.
Reply to: Comments on “Nomograms based on inflammatory biomarkers for predicting tumor grade and micro-vascular invasion in stage I/II hepatocellular carcinoma”
Authors: Li Peng , Huang Wei , Wang Feng , Ke Ye-Fang , Gao Lin , Shi Ke-Qing , Zhou Meng-Tao , Chen Bi-Cheng 
Published: November 2019

Abstract
We appreciate to receive commentary from Dr Guangtong Deng and Dr Liang Xiao to our article, “Nomograms based on inflammatory biomarkers for predicting tumor grade and micro-vascular invasion in stage I/II hepatocellular carcinoma”. First, neutrophil-to-lymphocyte ratio (NLR) and derived NLR (dNLR) are two different parameters. Some studies show that NLR is inconsistent with dNRL in prognostic value through multivariate Cox regression, therefore, it is reasonable that both NLR and dNLR entered into multivariate analysis simultaneously. Second, it is common that articles of predictive nomograms turned continuous variables into categorical variables. The reason is that the categorization of patient clinical variables is beneficial to doctors to make decisions based on the risk level of individual patients in clinical. At last, multicenter validation is quite difficult and we have listed the shortcomings in the limitations of our article. Further validation will need the joint efforts by other institutions.
Rhodiola rosea L., Rosenwurz
Authors: Salamani Egkina , Melzig Matthias F 
Published: November 2019

Zusammenfassung
Rhodiola rosea L., Rosenwurz, ist eine ausdauernde Pflanze aus der Familie der Crassulaceae, die v. a. in der nördlichen Hemisphäre wächst. Der Rosenwurz-Wurzelstock enthält u. a. Phenylethanoide (Salidrosid und Tyrosol) und Phenylpropanoide (Rosavin, Rosin und Rosarin). Diese Stoffe haben antioxidative, antiinflammatorische sowie neuro-, kardio- und hepatoprotektive Eigenschaften. Volksheilkundlich wird die Pflanze in Europa und Asien als Adaptogen gegen Stress und Müdigkeit eingesetzt.
Position Paper of the Task Force for Obstetrics and Prenatal Medicine (AGG – Section Preterm Birth) on the Placement, Removal and Surveillance of the Arabin Cervical Pessary in Patients at Risk for Spontaneous Preterm Birth
Authors: Kyvernitakis Ioannis , Maul Holger , Rath Werner , Kraft Katrina , Kuon Ruben , Hamza Amr , Reuschel Edith , Filsinger Barbara , Abele Harald , Garnier Yves , Bahlmann Franz , Schleußner Ekkehard , Berger Richard 
Published: November 2019

AbstractThis position paper describes clinically important, practical aspects of cervical pessary treatment. Transvaginal ultrasound is standard for the assessment of cervical length and selection of patients who may benefit from pessary treatment. Similar to other treatment modalities, the clinical use and placement of pessaries requires regular training. This training is essential for proper pessary placement in patients in emergency situations to prevent preterm delivery and optimize neonatal outcomes. Consequently, pessaries should only be applied by healthcare professionals who are not only familiar with the clinical implications of preterm birth as a syndrome but are also trained in the practical application of the devices. The following statements on the clinical use of pessary application and its removal serve as an addendum to the recently published German S2-consensus guideline on the prevention and treatment of preterm birth.
“My Shakespeare, rise”
Authors: Calvo Clara 
Published: November 2019

Abstract
This chapter focuses on the book Mr. William Shakespeare’s
Comedies, Histories and Tragedies (1623), which is widely known
today as the First Folio. When the First Folio was printed in 1623, the
editors and printers included in its preliminary matter several poems in
praise of Shakespeare. Among these were two by the author’s friend and
rival, Ben Jonson. One of the poems, “To the Memory of My Beloved, the
Author”, displays an unusual pronominal density and contains some intriguing
pronominal choices. This chapter examines the rhetorical fabric of Jonson’s
eulogy for Shakespeare and dissects the critical and ideological
implications of its peculiar use of the pronominal system. It focuses in
particular on the use of first person possessive pronouns such as “my” and
“ours” in relation to Jonson’s personal agenda: his self-fashioning as
literary critic and his support, as Poet Laureate in all but name, for King
James’s vision of a united Britain.
Doing and teaching
Authors: Toolan Michael 
Published: November 2019

Abstract
This chapter explores the continuity between Bill Nash’s academic
work on style and stylistics and his fiction writing. In both forms, Nash
aimed to instruct and entertain, and saw that to achieve those ends one had
to be seriously playful and use a creative imagination. The chapter focusses
specifically on the stylistic means by which Nash closes his short novel,
Kettle of Roses, a novel that takes the form of
eighteen expansive letters from Edna Pugh to a childhood friend, reporting
the recent developments in Edna’s life. There seems no decisive basis in
plot or logic for the letters to leave off where they do, so arguably Nash
is faced with a problem: how to bring the novel to a satisfying close. The
author shows how stylistic analysis of a paragraph in the final letter
highlights the presence of many of the features he has called High Emotional
Involvement (HEI) narration, a style of narration that creates a more
intense engagement, emotionally and ethically, in the story situation than
is encountered elsewhere in the narrative. The author has found HEI
narration used near the close of many modern short stories, where it seems
to be used in part to make the imminent ending satisfying and acceptable to
the reader. It serves a similar function in Kettle of
Roses.
Fact, fiction and French flights of fancy
Authors: Stubbs Michael 
Published: November 2019

Abstract
This chapter discusses a satirical novel: La septième
fonction du langage by Laurent Binet, published in 2015. The
book is a thriller with a deliberately absurd plot about a search for a lost manuscript which holds the secret of ultimate rhetorical power: the ability to convince anyone to
do anything. Although the characters in the novel include some “real
people”, such as two former Presidents of France, Stubbs argues that
Binet’s characters in general embody an extreme mix of factual and fictional
characteristics, and the merciless satire expressed in their mixed
ontological status has left many ordinary readers and professional critics
uncertain how to evaluate the novel. Stubbs employs various models of
analysis, including John Searle’s observations on the logical status of
fictional discourse, and contends that, while useful, this approach does not
explain in full how readers distinguish fact from fiction, nor indeed how
far writers can appropriately go with outrageous caricatures of living
persons. In sum, the chapter shows how the novel provides textual problems
which have not been solved by either literary scholars or language
philosophers.
“Americans don’t do Irony”
Authors: Simpson Paul 
Published: November 2019

Abstract
This chapter probes the common (and perhaps controversial)
perception of many in the UK and Ireland that people from North America
“don’t do” irony. Stimulated by the type of discussion found in Nash’s The Language of
Humour (1985), the author interrogates this folk
belief by developing a quantitative methodology to capture the ways in which
ironic situations are interpreted by people from diverse national
backgrounds. This methodology comprises an anonymous online experiment which
gathers reactions to six narrative scenarios from over 300 informants
world-wide. Each informant is required to provide a one-word response to
each scenario after which they may offer an optional, longer free-text
commentary on the same story. In the course of the chapter, the author
advances a theoretical model of situational irony, while the results
elicited from the survey shed some light on what people from different parts
of the world understand as an ironic situation.
A

SO(1,3)

gauge theory of quantum gravity: quantization of the non-interacting field
Authors: Wiesendanger C 
Published: November 2019

Abstract
The non-interacting field belonging to a new SO(1,3) gauge field theory equivalent to general relativity is canonically quantized in the Lorentz gauge and the physical Fock space

for non-interacting gauge particles is constructed. To assure both Lorentz covariance and positivity of the norm and energy expectation value for physical states restrictions needed in the construction of the physical Fock space are put consequentially on state vectors, and not on the algebra of creation and annihilation operators as usual—alltogether providing the second step in consistently quantizing gravitation
Lateral β-Ga2O3 field effect transistors
Authors: Chabak Kelson D , Leedy Kevin D , Green Andrew J , Mou Shin , Neal Adam T , Asel Thaddeus , Heller Eric R , Hendricks Nolan S , Liddy Kyle , Crespo Antonio , Miller Nicholas C , Lindquist Miles T , Moser Neil A , Fitch Robert C , Walker Dennis E , Dorsey Donald L , Jessen Gregg H 
Published: November 2019

Abstract
Beta phase Gallium Oxide (BGO) is an emerging ultra-wide bandgap semiconductor with disruptive potential for ultra-low power loss, high-efficiency power applications. The critical field strength is the key enabling material parameter of BGO which allows sub-micrometer lateral transistor geometry. This property combined with ion-implantation technology and large area native substrates result in exceptionally low conduction power losses, faster power switching frequency and even radio frequency power. We present a review of BGO epitaxial materials and lateral field-effect transistors developments, highlight early achievements and discuss engineering solutions with power switching and radio frequency applications in mind.