bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk factors and clinical characteristics of recurrent ectopic pregnancy: A case–control study
Authors: Wang Xinyan , Huang Lu , Yu Yan , Xu Sheng , Lai Yucheng , Zeng Wenjie 
Published: April 2020

AbstractObjectiveTo compare signs and symptoms between patients with recurrent ectopic pregnancies (REP) and primary ectopic pregnancies (PEP) and to identify potential risk factors of REP.Materials and MethodsData from 2014 to 2016 were analyzed. The study included 81 women each diagnosed with REP and PEP with no recurrence of ectopic pregnancy (EP) before January 2019. Information, including historical factors and findings at presentation of both group were collected. Data were compared between the two groups. Associations between REP and the risk factors were analyzed by logistic regression.ResultsThe findings revealed that compared to the patients in the PEP group, REP patients had significantly lower education (P = 0.001), higher proportion of previous infertility (P < 0.001) and different methods of PEP treatment (P = 0.001). Clinical data of the last operation revealed significantly higher occurrences of pelvic and peritubal adhesions (P < 0.05). Further multiple regression analysis showed that lower educational background (odds ratio [OR] = 4.183 95% confidence interval [CI] 1.311–13.344 P = 0.016), nulliparity (OR = 12.312 95% CI 3.382–44.824 P < 0.001), history of salpingotomy (OR = 7.129 95% CI 1.022–49.748 P < 0.05) and abortion (OR for one abortion = 21.576, P = 0.001; OR for two abortions =36.794, P < 0.001; OR for three abortions or more = 119.013, P < 0.001) were significant risk factors for REP.ConclusionActive education on contraception is required for patients with lower educational level and history of abortion. Different plans should be formulated for patients with EP. For EP patients wanting fertility, the risk between fertility preservation and REP needs to be evaluated as reproductive function cannot be pursued blindly while ignoring the risk of recurrence.
Dyadic support exchange and work engagement: An episodic test and expansion of self‐determination theory
Authors: Zeijen Marijntje E.L. , Petrou Paraskevas , Bakker Arnold B. , van Gelderen Benjamin R. 
Published: April 2020

According to the self‐determination theory (SDT), individuals flourish when they satisfy their psychological needs. We expand this proposition by testing whether employees satisfy their own needs and improve their own work engagement by providing support to their co‐workers. Moreover, we argue that it matters when and to whom the support is provided. We contend that the indirect effect of autonomously motivated support provision on the provider’s work engagement through the provider’s need satisfaction is stronger (1) during episodes that the receiver’s emotional demands are high (vs. low), (2) when the receiver’s learning goal orientation is high (vs. low), or (3) when the receiver’s prove performance goal orientation is low (vs. high). We collected data among 97 dyads of police officers (N = 194 participants) during two time blocks on one working day (N = 227–491 episodes). Multi‐level analyses confirmed that support provision related positively to the provider’s episodic work engagement through episodic need satisfaction. As hypothesized, this indirect relationship was stronger during emotionally demanding episodes, or when the receiver was characterized by a low prove performance goal orientation. Learning goal orientation did not moderate the support provision–work engagement relationship. These findings expand SDT by indicating that individuals satisfy their own daily needs by providing support, and by showing that it matters when and to whom support is provided.Practitioner points

Providing help benefits both the beneficiary and the helper

Managers should encourage the daily exchange of social resources between employees

The exchange of social support between co‐workers is crucial when employees face demanding clients
Value of colchicine as treatment for recurrent oral ulcers: A systematic review
Authors: Cabras Marco , Carrozzo Marco , Gambino Alessio , Broccoletti Roberto , Sciascia Savino , Baldovino Simone , Arduino Paolo Giacomo 
Published: April 2020

AbstractBackgroundIn oral medicine, colchicine is a therapeutic alternative for idiopathic recurrent aphthous stomatitis (RAS), Behçet disease (BD), periodic fever, aphthous stomatitis, pharyngitis and cervical adenitis (PFAPA) syndrome, and mouth and genitals ulcers with inflamed cartilage (MAGIC) syndrome. The present review aims to evaluate reliability of colchicine against recurrent oral ulcers.MethodsA systematic review was conducted, with the following PICO (Patient, Intervention, Control, Outcome) question: “In populations with idiopathic or secondary recurrent oral ulcers, is colchicine more effective in improving pain and accelerating healing, compared to other intervention or placebo?”ResultsSeven RCTs and 3 OCTs were considered eligible. Four RCTs focused on BD, two RCTs and three OCTs on RAS, and one RCT on PFAPA syndrome. Heterogeneity between RCTs prevented from meta‐analysis. Regarding BD, no significant difference between colchicine and placebo was found in two of three placebo‐controlled RCTs, whereas the third RCT showed benefit. A comparative RCT found ciclosporin more effective than colchicine for oral lesions of BD. One open‐label RCT showed promising but partial results on colchicine in reducing PFAPA attacks, when compared to corticosteroids. Concerning RAS, colchicine appeared less effective than clofazimine, thalidomide and dapsone, and with outcomes similar to low‐dosage corticosteroids but higher gastric discomfort than prednisolone. One OCT reported positive results compared with no treatment but a RCT found no difference with placebo.ConclusionRole of colchicine as treatment for idiopathic or secondary recurrent oral ulcers is still controversial. Further standardized RCTs and crossover trials are needed.
Development of a Checklist to Prevent Reconstructive Errors Made By Undergraduate Dental Students
Authors: Almufleh Balqees , Ducret Maxime , Malixi Jodeci , Myers Jeffrey , Nader Samer Abi , Franco Echevarria Maria , Adamczyk Jessica , Chisholm Alicia , Pollock Natalie , Emami Elham , Tamimi Faleh 
Published: April 2020

AbstractPurposeTo design a checklist in order to reduce the frequency of reconstructive preventable errors (PE) performed by undergraduate dental students at McGill University.Materials and MethodsThe most common PE occurring at a university dental clinic were identified by three reviewers analyzing the refunded cases, and used to create a preliminary checklist. This checklist was then validated by a panel of dental educators to produce a finalized 20‐item checklist. The 20‐question checklist was then submitted to students in a cross‐sectional survey‐based study to evaluate its relevance to undergraduate clinical education needs.ResultsAs many as 81% of students reported to have forgotten at least one item of the checklist during care of their last patient, and the most forgotten checklist items corresponded to the pretreatment stage. The students also reported that 17 of the 20 items in the checklist were relevant to a considerable extent or highly relevant.ConclusionCommon PE identified in the undergraduate clinic could be used to create a checklist of relevant items designed to reduce errors made by students and practitioners performing prosthodontic and reconstructive treatments. However, further studies are required to evaluate the implementation and efficiency of the checklist.
Detection and molecular characterization of carbendazim‐resistant Colletotrichum truncatum Isolates causing anthracnose of soybean in Thailand
Authors: Poti Teeranai , Mahawan Kanyarat , Cheewangkoon Ratchadawan , Arunothayanan Hatthaya , Akimitsu Kazuya , Nalumpang Sarunya 
Published: April 2020

AbstractA loss of fungicide efficacy, particularly for carbendazim, was noted in soybean fields in Thailand and was considered to be due to the development of Colletotrichum truncatum resistance. The carbendazim sensitivity of C. truncatum populations isolated from various soybean fields in Thailand was thus evaluated with in vitro sensitivity assays and molecular characterization of mutations in the sequences of the ß2‐tubulin (TUB2) gene that confer carbendazim resistance in the pathogen. Among 52 isolates, 46 isolates were classified as highly resistant (HR) to carbendazim (EC50 > 1,000 µg/ml). All HR isolates grew on PDA amended with carbendazim at 1,000 µg/ml. Six isolates were classified as carbendazim sensitive (S) (EC50 < 1 µg/ml). Mycelial growth on PDA amended with 1 µg/ml carbendazim was inhibited by over 50% compared with growth on PDA alone. When a partial TUB2 gene from the isolates was amplified and analysed using predicted amino acid sequences, an alteration from glutamic acid to alanine at codon 198 (E198A) was found in 45 HR isolates for which the EC50 was higher than 2000 µg/ml. This mutation resulted from a nucleotide substitution from adenine to cytosine (GAG → GCG). The other HR isolate, CtPhS_1, with EC50 of 1,127 µg/ml, had an alteration at codon 200 (F200Y) (TTC → TAC).
Pre‐cold acclimation promotes cryoprotectant accumulation and enhances freezing tolerance of Meloidogyne incognita
Authors: Wang Yuanzheng , Liu Chen , Ren Ping , Li Yingmei 
Published: April 2020

AbstractStrong evidence suggests that cryoprotectant accumulation during pre‐cold acclimation protects cells against freezing injuries caused by cellular dehydration. In this study, the concentrations of trehalose and glycerol were measured in Meloidogyne incognita and it was found that both cryoprotectants were significantly accumulated in second‐stage juveniles (J2) of M. incognita after acclimation at 4°C. However, compared with non‐acclimated samples, only a higher level of trehalose was induced in the egg masses of M. incognita in response to cold treatment. Further characterizations indicated that pre‐cold acclimation efficiently accelerated the speed of larvae hatching from egg masses that were subjected to freezing at −1°C. In addition, the survival rate and pathogenicity of M. incognita J2 that had been acclimated prior to freezing were significantly enhanced when compared with non‐acclimated J2 individuals. As far as we know, this is the first time that this phenomenon has been reported in M. incognita.
Self‐assessed impact of oral health on the psychological well‐being and depressive symptoms of older adults living in Melbourne
Authors: Mariño Rodrigo , Enticott Joanne , Browning Colette , Elsamman Mahmoud , Etzion Rachel , Ferooz Maryam , Fujihara Ryuun , Hancock Hugo , He Julian , Kendig Hal 
Published: April 2020

AbstractObjectivesThis study aimed to investigate oral health‐related factors affecting the self‐assessed psychological well‐being (PW) and depressive symptoms of independent‐living Australians aged 79 years and over living in the community in metropolitan Melbourne.MethodsThe Melbourne Longitudinal Studies on Healthy Aging (MELSHA) program was used as the data source in this study and includes data on the health and well‐being of older participants. The MELSHA baseline data collection occurred in 1994, the current study used data from the 2008 data collection and included 201 participants, who remained in the study. Data were analyzed using multiple linear regression (MLR) analysis with a stepwise procedure to identify the variables that accounted for a significant proportion of the variance in the participants' PW scores.ResultsPresent findings indicate that oral health may play a significant mediating role in PW through maintaining a presentable and acceptable physical appearance. Some 16.4 percent of participants reported feeling concerned about their dental appearance, either “Sometimes,” “Often,” or “Very often.” Multivariate analysis showed significantly influences on PW positive and negative affect scores (P < 0.0001); and depressive symptoms (P < 0.0001) by participants' dentition status, enjoyment of meals, self‐reported feeling of concern about the appearance of the mouth, social activity and self‐assessment of general health. Final models explained 17.8, 20.1, and 24.6 percent of the variance of PW positive, negative affect scores, and depressive symptoms, respectively.ConclusionsOral health, specifically the appearance of the mouth and dentition, plays a significant role in the PW of older Melbournians. Future cross‐sectional and longitudinal studies are indicated to raise awareness on the changes required to improve the quality of life of the older population.
Measuring melatonin by immunoassay
Authors: Kennaway David J. 
Published: April 2020

AbstractThe pineal gland hormone melatonin continues to be of considerable interest to biomedical researchers. Of particular interest is the pattern of secretion of melatonin in relation to sleep timing as well as its potential role in certain diseases. Measuring melatonin in biological fluids such as blood and saliva presents particular methodological challenges since the production and secretion of the hormone are known to be extremely low during the light phase in almost all situations. Active secretion only occurs around the time of lights out in a wide range of species. The challenge then is to develop practical high‐throughput assays that are sufficiently sensitive and accurate enough to detect levels of melatonin less than 1 pg/mL in biological fluids. Mass spectrometry assays have been developed that achieve the required sensitivity, but are really not practical or even widely available to most researchers. Melatonin radioimmunoassays and ELISA have been developed and are commercially available. But the quality of the results that are being published is very variable, partly not only because of poor experimental designs, but also because of poor assays. In this review, I discuss issues around the design of studies involving melatonin measurement. I then provide a critical assessment of 21 immunoassay kits marketed by 11 different companies with respect to validation, specificity and sensitivity. Technical managers of the companies were contacted in an attempt to obtain information not available online or in kit inserts. A search of the literature was also conducted to uncover papers that have reported the use of these assays, and where possible, both daytime and night‐time plasma or saliva melatonin concentrations were extracted and tabulated. The results of the evaluations are disturbing, with many kits lacking any validation studies or using inadequate validation methods. Few assays have been properly assessed for specificity, while others report cross‐reaction profiles that can be expected to result in over estimation of the melatonin levels. Some assays are not fit for purpose because they are not sensitive enough to determine plasma or saliva DLMO of 10 and 3 pg/mL, respectively. Finally, some assays produce unrealistically high daytime melatonin levels in humans and laboratory animals in the order of hundreds of pg/mL. In summary, this review provides a comprehensive and unique assessment of the current commercial melatonin immunoassays and their use in publications. It provides researchers new to the field with the information they need to design valid melatonin studies from both the perspective of experimental/clinical trial design and the best assay methodologies. It will also hopefully help journal editors and reviewers who may not be fully aware of the pitfalls of melatonin measurement make better informed decisions on publication acceptability.
The Solanum glaucophyllum Desf. extract reduces mineralized matrix synthesis in osteogenically differentiated rat mesenchymal stem cells in vitro
Authors: Melo Fabrício Gomes , Ocarino Natália Melo , Reis Amanda Maria Sena , Gimeno Eduardo Juan , Massone Adriana Raquel , Melo Marília Martins , Botelho Ana Flávia Machado , Stehmann João Renato , Serakides Rogéria 
Published: April 2020

AbstractThe Solanum glaucophyllum Desf. has been used to treat and prevent diseases in human and veterinary medicine. On the other hand, plant poisoning causes several bone diseases, among them osteoporosis, which is characterized by osteoblastic hypoplasia. Because the osteoblast is a cell derived from the differentiation of mesenchymal stem cells (MSCs) from bone marrow, the hypothesis is that the plant reduces the osteogenic differentiation of MSCs. The objective of this study was to evaluate the effects of S. glaucophyllum Desf. extract on MSCs cultured in osteogenic differentiation medium. We determined by liquid chromatography that 1 ml of plant extract contained 3.8 μl of 1,25(OH)2D3 (calcitriol). Four groups of MSCs cultivated in osteogenic medium were evaluated as follows: (a) treated with 100 μl of extract/L containing 0.4 μg/L of calcitriol; (b) treated with 1 ml of extract/L containing 4 μg/L of calcitriol; (c) treated with 5 ml of extract/L containing 20 μg/L of calcitriol; and (d) a control group without extract. We performed alkaline phosphatase activity assay, analysis of MTT conversion to formazan, and evaluated the percentage of cells, and number and diameter of mineralization nodules. The expression of gene transcripts for osteopontin, bone sialoprotein and BMP‐2 was analysed by RT‐qPCR. After 21 days, there was a significant reduction in MTT conversion to formazan in treated groups, of the cellularity in the group with 5 ml of extract/L, and in the number and size of mineralization nodules in the groups treated with 1 and 5 ml of extract/L. The 5 ml extract/L concentration also reduced transcript expression of osteopontin. It is concluded that S. glaucophyllum Desf. at concentrations of 1 and 5 ml extract/L reduced mineralized matrix synthesis in MSCs cultivated in osteogenic differentiation medium, which suggests that this is one of the mechanisms by which osteoporosis occurs in intoxicated animals.
Actigraphy measurement of physical activity and energy expenditure in narcolepsy type 1, narcolepsy type 2 and idiopathic hypersomnia: A Sensewear Armband study
Authors: Bornstein Axel , Hedström Anders , Wasling Pontus 
Published: April 2020

AbstractThe cause of obesity in narcolepsy type 1 (NT1) patients is not fully understood. The present study investigated if a reduced physical activity could explain weight gain in NT1. Seventy‐nine patients were included in this retrospective study and divided into an NT1 group (n = 56) and a non‐NT1 group (n = 23), including NT2 and idiopathic hypersomnia (IH). Accelerometry‐derived measures of physical activity, total energy expenditure and skin temperature were collected from patients during seven consecutive days without medication. In addition, results from multiple sleep latency tests and the Epworth Sleepiness Scale questionnaire, body weight, height and CSF orexin/hypocretin were acquired. Three measurements of physical activity, including metabolic equivalent of task (MET), the average time of physical activity and step count, were compared without differences between groups. Neither could we find a significant difference in total energy expenditure or skin temperature. Thus, by analysing accelerometric data, we could not find any differences in the amount of physical activity or total energy expenditure explaining overweight in NT1.
Influence of heat treatment of goat milk on casein micelle size, rheological and textural properties of acid gels and set type yoghurts
Authors: Hovjecki Marina , Miloradovic Zorana , Rac Vladislav , Pudja Predrag , Miocinovic Jelena 
Published: April 2020

AbstractAcid gels and yoghurts were made from goat milk that was heated at 72°C/30 s, 85°C/5 min, and 95°C/5 min, followed by acidification with starter culture at 43C until pH 4.6. The rheological and textural properties of acid gels and yoghurts were analyzed using dynamic low amplitude oscillatory rheology and back extrusion texture analysis, respectively. The effect of goat milk heat treatment on the mean casein micelle diameter and protein profile was also determined by dynamic light scattering and SDS PAGE electrophoresis, respectively. The shortest gelation and fermentation time was recorded for yoghurt prepared from milk heated at 85°C/5 min. Also, the pH of gelation, the storage moduli (G′) and yield stress were higher for this yoghurt, compared with the other two. Textural properties of goat milk yoghurts such as firmness and consistency were strongly affected by milk heat treatment, and the highest values were recorded for yoghurt produced from milk preheated at 85°C/5 min, as well. The largest casein micelles were measured after 85°C/5 min treatment and their size decreased at higher temperature, despite higher denaturation of whey proteins at the most intense heat regime, indicating the structure changes that influence on the acid gelation.
Identification of hepatitis B virus aetiologic antigens, HBx and Pre‐S2, in diffuse large B‐cell lymphoma
Authors: Huang Xinxing , Young Ken H. , Guo Wei , Wang Yinping , Wang Xueju , Xi Yanfeng , Wang Lifen , Bai Ou 
Published: April 2020

AbstractDiffuse large B‐cell lymphoma (DLBCL) has been reported to have a significant association with the hepatitis B virus (HBV) infection. However, there has been no experimental evidence to determine whether the components of the hepatitis B virus are expressed in lymphoid cells. In this study, we used immunohistochemical methods to explore whether the antigens of hepatitis B virus are expressed in DLBCL lymphoma cells in HBsAg‐positive DLBCL patients (HBsAg + DLBCL). HBx antigen was detected in 48.9% of HBsAg + DLBCL patients, and the expression rate of the Pre‐S2 antigen was 57.2%. HBx expression was significantly associated with high‐level expression of c‐Myc, while the Pre‐S2 antigen was not. In this study, we demonstrated that HBx antigen and Pre‐S2 antigen could be detected in lymphoma cells, and HBx expression was related to c‐Myc expression. Our findings provide a strong basis for further study of the HBV‐infected DLBCL and molecular mechanism underlying the lymphomagenesis.
Pharmacokinetics of ceftiofur sodium in Peekapoo dogs following a single intravenous and subcutaneous injection
Authors: Yang Fang , Yang Fan , Wang Han , Zhang Chao‐Shuo , Song Zhe‐Wen , Shao Hao‐Tian , Zhang Mei 
Published: April 2020

AbstractThe present study aimed to determine the pharmacokinetic profiles of ceftiofur (as measured by ceftiofur and its active metabolites concentrations) in a small‐size dog breed, Peekapoo, following a single intravenous or subcutaneous injection of ceftiofur sodium. The study population comprised of five clinically healthy Peekapoo dogs with an average body weight (BW) of 3.4 kg. Each dog received either intravenous or subcutaneous injection, both at 5 mg/kg BW (calculated as pure ceftiofur). Plasma samples were collected at different time points after the administration. Ceftiofur and its active metabolites were extracted from plasma samples, derivatized, and further quantified by high‐performance liquid chromatography. The concentrations versus time data were subjected to noncompartmental analysis to obtain the pharmacokinetic parameters. The terminal half‐life (t1/2λz) was calculated as 7.40 ± 0.79 and 7.91 ± 1.53 hr following intravenous and subcutaneous injections, respectively. After intravenous treatment, the total body clearance (Cl) and volume of distribution at steady‐state (VSS) were determined as 39.91 ± 4.04 ml hr−1 kg−1 and 345.71 ± 28.66 ml/kg, respectively. After subcutaneous injection, the peak concentration (Cmax; 10.50 ± 0.22 μg/ml) was observed at 3.2 ± 1.1 hr, and the absorption half‐life (t1/2ka) and absolute bioavailability (F) were calculated as 0.74 ± 0.23 hr and 91.70%±7.34%, respectively. The pharmacokinetic profiles of ceftiofur and its related metabolites demonstrated their quick and excellent absorption after subcutaneous administration, in addition to poor distribution and slow elimination in Peekapoo dogs. Based on the time of concentration above minimum inhibitory concentration (T > MIC) values calculated here, an intravenous or subcutaneous dose at 5 mg/kg of ceftiofur sodium once every 12 hr is predicted to be effective for treating canine bacteria with a MIC value of ≤4.0 μg/ml.