bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of nicotinamide adenine dinucleotide phosphate oxidase 4 on migration and invasion of fibroblast-like synoviocytes in rheumatoid arthritis
Authors: Lee Ha-Reum , Yoo Su-Jin , Kim Jinhyun , Yoo In Seol , Park Chan Keol , Kang Seong Wook 
Published: May 2020

Abstract
Background
Reactive oxygen species (ROS) regulate the migration and invasion of fibroblast-like synoviocytes (FLS), which are key effector cells in rheumatoid arthritis (RA) pathogenesis. Nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) induces ROS generation and, consequently, enhances cell migration. Despite the important interrelationship between RA, FLS, and ROS, the effect of NOX4 on RA pathogenesis remains unclear.

Methods
FLS isolated from RA (n = 5) and osteoarthritis (OA, n = 5) patients were stimulated with recombinant interleukin 17 (IL-17; 10 ng/ml) and tumor necrosis factor alpha (TNF-α; 10 ng/ml) for 1 h. Cell migration, invasion, adhesion molecule expression, vascular endothelial growth factor (VEGF) secretion, and ROS expression were examined. The mRNA and protein levels of NOX4 were analyzed by RT-qPCR and western blotting, respectively. The NOX4 inhibitor GLX351322 and NOX4 siRNA were used to inhibit NOX4 to probe the effect of NOX4 on these cellular processes.

Results
Migration of RA FLS was increased 2.48-fold after stimulation with IL-17 and TNF-α, while no difference was observed for OA FLS. ROS expression increased in parallel with invasiveness of FLS following cytokine stimulation. When the expression of NOX was examined, NOX4 was significantly increased by 9.73-fold in RA FLS compared to unstimulated FLS. Following NOX4 inhibition, cytokine-induced vascular cell adhesion molecule 1 (VCAM1), VEGF, and migration and invasion capacity of RA FLS were markedly decreased to unstimulated levels.

Conclusion
NOX4 is a key contributor to cytokine-enhanced migration and invasion via modulation of ROS, VCAM1, and VEGF in RA FLS.
The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditions
Authors: Hu Xinyu , Zeng Chunlin , Hou Zhaozhi , Wang Yuan , Xu Qin , Isobe Kazuo , Senoo Keishi , Zhu Lin 
Published: May 2020

AbstractPurposeAs a potential tool for the biodegradation of nitrogen contaminants, including nitrate, nitrite, and ammonium, in pickled foods with high salinity, the halophilic and denitrifying archaeal strainHalomicrobiumsp. ZPS1 was isolated from edible salt particles.MethodsUnder anaerobic and static culture conditions,Halomicrobiumsp. ZPS1 could simultaneously degrade nitrate, nitrite, and ammonium in liquid medium with 18% salinity and generate N2O. To gain insight into these physiological characteristics, the complete genome ofHalomicrobiumsp. ZPS1 was sequenced to reveal the mechanism of nitrogen metabolism associated with salt-tolerance.ResultThe complete genome sequencing revealed a genome size of 3,094,203 bp with a circular chromosome and a GC content of 65.64%. Based on gene annotation, 3191 CDSs, 6 rRNA genes, and 76 tRNA genes were identified. Moreover, 28 genes were annotated as related to salt tolerance, ammonium assimilation, and a truncated denitrification pathway.ConclusionThe annotated functional genes indicate thatHalomicrobiumsp. ZPS1 could be a candidate strain for the simultaneous removal of nitrate, nitrite, and ammonia in extremely high salt environments.
The role of hepatocyte growth factor in mesenchymal stem cell-induced recovery in spinal cord injured rats
Authors: Song Peiwen , Han Tianyu , Xiang Xia , Wang Ying , Fang Huang , Niu Yang , Shen Cailiang 
Published: May 2020

Abstract
Background
Mesenchymal stem cells (MSCs) have become a promising treatment for spinal cord injury (SCI) due to the fact that they provide a favorable environment. Treatment using MSCs results in a better neurological functional improvement through the promotion of nerve cell regeneration and the modulation of inflammation. Many studies have highlighted that the beneficial effects of MSCs are more likely associated with their secreted factors. However, the identity of the factor that plays a key role in the MSC-induced neurological functional recovery following SCI as well as its molecular mechanism still remains unclear.

Methods
A conditioned medium (collected from the MSCs) and hepatocyte growth factor (HGF) were used to test the effects on the differentiation of neural stem cells (NSCS) in the presence of BMP4 with or without a c-Met antibody. In SCI rats, Western blot, ELISA, immunohistochemistry, and hematoxylin-eosin staining were used to investigate the biological effects of MSC-conditioned medium and HGF on nerve cell regeneration and inflammation with or without the pre-treatment using a c-Met antibody. In addition, the possible molecular mechanism (cross-talk between HGF/c-Met and the BMP/Smad 1/5/8 signaling pathway) was also detected by Western blot both in vivo and in vitro.

Results
The conditioned medium from bone marrow-derived MSCs (BMSCs) was able to promote the NSC differentiation into neurons in vitro and the neurite outgrowth in the scar boundary of SCI rats by inhibiting the BMP/Smad signaling pathway as well as reduces the secondary damage through the modulation of the inflammatory process. The supplementation of HGF showed similar biological effects to those of BMSC-CM, whereas a functional blocking of the c-Met antibody or HGF knockdown in BMSCs significantly reversed the functional improvement mediated by the BMSC-CM.

Conclusions
The MSC-associated biological effects on the recovery of SCI rats mainly depend on the secretion of HGF.
Physiological and subjective burden when wearing fire protective boots between 3.2 and 5.3 kg
Authors: Roh Sang-Hyun , Ko Yelin , Lee Joo-Young 
Published: May 2020

AbstractThis study investigated the effects of weight increase of firefighters’ boots on physiological and psychological strain. Seven young males (70.9 ± 4.8 kg in body mass, BM) participated in the following four boot conditions while wearing standard firefighting personal protective equipment: 3.2, 3.9, 4.6, and 5.3 kg (4.5, 5.5, 6.5, and 7.5%BM). The results showed that the four boot conditions resulted in no differences in rectal temperature, mean skin temperature, energy expenditure and overall thermal comfort during walking, while increments in heart rate were greater for 5.3 kg than for other three conditions (P < 0.05). Subjects felt less warm and had less uncomfortable feet during exercise for the 3.2 kg condition compared to the three other heavier conditions (P < 0.05). These results indicate that psychological strain due to the load carried on the feet appeared earlier (between 4.5 to 5.5%BM) than physiological strain in terms of heart rate (between 6.5 to 7.5%BM). We finally suggest a 5% body mass upper limit for boot weight because subjective strain of the feet may be a valuable preliminary alarm for the physiological strain of firefighters wearing heavy boots.
Scaling the impact of sustainability initiatives: a typology of amplification processes
Authors: Lam David P. M. , Martín-López Berta , Wiek Arnim , Bennett Elena M. , Frantzeskaki Niki , Horcea-Milcu Andra I. , Lang Daniel J. 
Published: May 2020

AbstractAmplifying the impact of sustainability initiatives to foster transformations in urban and rural contexts, has received increasing attention in resilience, social innovation, and sustainability transitions research. We review the literature on amplification frameworks and propose an integrative typology of eight processes, which aim to increase the impact of such initiatives. The eight amplification processes are: stabilizing, speeding up, growing, replicating, transferring, spreading, scaling up, and scaling deep. We aggregated these processes into three categories: amplifying within, amplifying out, and amplifying beyond. This integrative typology aims to stimulate the debate on impact amplification from urban and rural sustainability initiatives across research areas to support sustainability transformations. We propose going beyond an understanding of amplification, which focuses only on the increase of numbers of sustainability initiatives, by considering how these initiatives create transformative change.
Connecting formal and informal spaces: a long-term and multi-level view of Medellín’s Metrocable
Authors: Galvin Madeleine , Maassen Anne 
Published: May 2020

AbstractThe inauguration of Medellín, Colombia’s aerial cable car in 2004, is widely seen as a key turning point in reversing the city’s historical reputation for drug and gang-related crimes towards greater inclusiveness and public safety. Analyses of Medellín’s transformation have tended to focus on establishing the immediate positive outcomes achieved from the cable car and assessing persistent inequality and the fragile balance between enfranchisement and top-down institutional control. In this paper, we take these interpretations of Medellín’s transformation as our starting point and propose that a lasting legacy is to be found in the way the city plans for and works in disinvested areas. Our focus is on examining the elements that have made transformation possible in Medellín. We begin by exploring a set of framing conditions during the period of 1991 to 2000 (‘Before Line K’) and then outline the implementation of Metrocable and its shorter-term outcomes (‘Executing Line K’), before finally reflecting on the wider transformative impacts of this experience (‘Beyond Line K’). As key takeaways, we highlight the role of national policy, municipal finance, and community engagement in bringing a highly informal space into the reach of public institutions, thus providing insights for urban decision-makers looking to do the same.
Abnormal bodily experiences detected by Abnormal Bodily Phenomena questionnaire are more frequent and severe in schizophrenia than in bipolar disorder with psychotic features
Authors: Stanghellini Giovanni , Palumbo Davide , Ballerini Massimo , Mucci Armida , Catapano Francesco , Giordano Giulia Maria , Galderisi Silvana 
Published: May 2020

Abstract

Background:
Patients with schizophrenia display experiential anomalies in their feelings and cognitions arising in the domain of their lived body. These abnormal bodily phenomena (ABP) are not part of diagnostic criteria for schizophrenia. One of the reasons is the difficulty to assess specific ABP for schizophrenia spectrum disorders. The present study aimed to explore the presence in patients with schizophrenia of specific ABP.

Methods:
We used a semistructured interview—the Abnormal Bodily Phenomena questionnaire (ABPq), an instrument devised to detect and measure ABP specific to patients with schizophrenia. Fifty-one outpatients affected by schizophrenia and 28 euthymic outpatients affected by bipolar disorder type I with psychotic features (BD-pf-e) were recruited. Before assessing the specificity for schizophrenia of the observed ABP, we tested the internal consistency and the convergent validity of the ABPq in patients with schizophrenia. Specificity was assessed by examining potential differences in ABPq among the patients with schizophrenia in remission (SCZ-r) and BD-pf-e.

Results:
The ABPq shows strong internal consistency and convergent validity. As to the specificity, ABP measured by ABPq were more frequent and severe in SCZ-r than in BD-pf-e. In particular, all ABPq dimensions, except “Coherence,” had at least mild severity in over 50% of SCZ-r, while dimensions with at least mild severity were observed in 5–10% of the BD-pf-e.

Conclusions:
These findings can contribute to establish more precise phenomenal boundaries between schizophrenia and bipolar disorder, to explore the borders between nonpsychotic and psychotic forms of ABP, between ABP and negative and disorganized symptoms, and to enlighten core aspects of schizophrenia.
Electronic Medical Record Search Engine (EMERSE): An Information Retrieval Tool for Supporting Cancer Research
Authors: Hanauer David A. , Barnholtz-Sloan Jill S. , Beno Mark F. , Del Fiol Guilherme , Durbin Eric B. , Gologorskaya Oksana , Harris Daniel , Harnett Brett , Kawamoto Kensaku , May Benjamin , Meeks Eric , Pfaff Emily , Weiss Janie , Zheng Kai 
Published: May 2020

PURPOSE The Electronic Medical Record Search Engine (EMERSE) is a software tool built to aid research spanning cohort discovery, population health, and data abstraction for clinical trials. EMERSE is now live at three academic medical centers, with additional sites currently working on implementation. In this report, we describe how EMERSE has been used to support cancer research based on a variety of metrics. METHODS We identified peer-reviewed publications that used EMERSE through online searches as well as through direct e-mails to users based on audit logs. These logs were also used to summarize use at each of the three sites. Search terms for two of the sites were characterized using the natural language processing tool MetaMap to determine to which semantic types the terms could be mapped. RESULTS We identified a total of 326 peer-reviewed publications that used EMERSE through August 2019, although this is likely an underestimation of the true total based on the use log analysis. Oncology-related research comprised nearly one third (n = 105; 32.2%) of all research output. The use logs showed that EMERSE had been used by multiple people at each site (nearly 3,500 across all three) who had collectively logged into the system > 100,000 times. Many user-entered search queries could not be mapped to a semantic type, but the most common semantic type for terms that did match was “disease or syndrome,” followed by “pharmacologic substance.” CONCLUSION EMERSE has been shown to be a valuable tool for supporting cancer research. It has been successfully deployed at other sites, despite some implementation challenges unique to each deployment environment.
Patients With Cancer and COVID-19: A WhatsApp Messenger-Based Survey of Patients’ Queries, Needs, Fears, and Actions Taken
Authors: Gebbia Vittorio , Piazza Dario , Valerio Maria Rosaria , Borsellino Nicolò , Firenze Alberto 
Published: May 2020

PURPOSE This descriptive investigation was undertaken at three oncology units to report queries, needs, and fears related to severe acute respiratory syndrome coronavirus 2 (COVID-19) of patients with cancer and to avoid uncontrolled treatment delays or withdrawal, behavioral mistakes, and panic. PATIENTS AND METHODS All queries spontaneously delivered through the WhatsApp instant messaging system commonly used by patients to communicate with oncology units were collected and grouped by homology in five categories. Responses to the queries were given according to recommendations by the Italian Association of Medical Oncology through WhatsApp and by subsequent phone calls. Patients were also classified according to the site of the primary tumor, stage of disease, and current treatments. Analysis of the association between these data and queries was carried out. RESULTS The social scenario in Italy is a nationwide lockdown except for hospitals, pharmacies, and food supplies. Overall, 446 different patients’ WhatsApp conversations were analyzed between March 1 and March 13 and comprised the following: requirement of visit delay by patients undergoing oral therapies or in follow-up, delays in chemotherapy or immunotherapy administration, queries about possible immunosuppression, and changes in lifestyle or daily activities. Delay requirements were statistically more frequent among patients with prostate or breast cancer compared with those with lung or pancreatic cancer. Actions taken by oncologists are also reported. CONCLUSION To our knowledge, the WhatsApp instant messaging system has been occasionally used in other medical settings with controversial results. In our experience, WhatsApp turned out to be adequate to give a rapid answer to most queries from patients with cancer in the COVID-19 pandemic scenario.
Cauliflower-Like Nanostructured Localized Surface Plasmon Resonance Biosensor Chip for Cytokine Detection
Authors: Luo Xi , Zhu Chen , Saito Masato , Espulgar Wilfred Villariza , Dou Xiaoming , Terada Yuhei , Obara Ain , Uchiyama Sachiyo , Tamiya Eiichi 
Published: May 2020

Abstract
Localized surface plasmon resonance (LSPR) based nano-plasmonic biosensors have attracted great attentions due to rapid detection and label-free capability. Aiming to obtain a high performance LSPR sensor chip, a cauliflower-like nanopillar (CLNP) structure was fabricated based on the cyclo-olefin polymer (COP) material which could increase the sensitivity according to the “hotspots” effect theory. An oxygen plasma etching procedure was introduced to the chip fabrication process to carve the nanopillar surface prepared by nanoimprinting lithography (NIL) into the cauliflower-like nanostructures. In this paper, the cauliflower-like nanopillar structured sensor chip was successfully obtained and the results confirmed that this sensor chip has higher sensitivity compared to an untreated nanopillar structured chip. Meanwhile, the biosensing capability was verified by cell interleukin-6 (IL-6) immunoassay. This approach provides an easy way to fabricate mass-producible LSPR biosensor chips for cell cytokine secretion detection.
Host deficiency in ephrin-A1 inhibits breast cancer metastasis
Authors: Shiuan Eileen , Inala Ashwin , Wang Shan , Song Wenqiang , Youngblood Victoria , Chen Jin , Brantley-Sieders Dana M. 
Published: May 2020

Background: The conventional dogma of treating cancer by focusing on the elimination of tumor cells has been recently refined to include consideration of the tumor microenvironment, which includes host stromal cells. Ephrin-A1, a cell surface protein involved in adhesion and migration, has been shown to be tumor suppressive in the context of the cancer cell. However, its role in the host has not been fully investigated. Here, we examine how ephrin-A1 host deficiency affects cancer growth and metastasis in a murine model of breast cancer. Methods: 4T1 cells were orthotopically implanted into the mammary fat pads or injected into the tail veins of ephrin-A1 wild-type (Efna1+/+), heterozygous (Efna1+/-), or knockout (Efna1-/-) mice. Tumor growth, lung metastasis, and tumor recurrence after surgical resection were measured. Flow cytometry and immunohistochemistry (IHC) were used to analyze various cell populations in primary tumors and tumor-bearing lungs. Results: While primary tumor growth did not differ between Efna1+/+, Efna1+/-, and Efna1-/- mice, lung metastasis and primary tumor recurrence were significantly decreased in knockout mice. Efna1-/- mice had reduced lung colonization of 4T1 cells compared to Efna1+/+ littermate controls as early as 24 hours after tail vein injection. Furthermore, established lung lesions in Efna1-/- mice had reduced proliferation compared to those in Efna1+/+ controls. Conclusions: Our studies demonstrate that host deficiency of ephrin-A1 does not impact primary tumor growth but does affect metastasis by providing a less favorable metastatic niche for cancer cell colonization and growth. Elucidating the mechanisms by which host ephrin-A1 impacts cancer relapse and metastasis may shed new light on novel therapeutic strategies.
Health technology assessment capacity at national level in sub-Saharan Africa: an initial survey of stakeholders
Authors: Hollingworth Samantha A. , Ruiz Francis , Gad Mohamed , Chalkidou Kalipso 
Published: May 2020

Background: Health technology assessment (HTA) is an effective tool to support priority setting (PS) in health. Stakeholder groups need to understand HTA appropriate to their role and to interpret and critique the evidence produced. We aimed to rapidly assess current health system priorities and policy areas of demand for HTA in Sub-Saharan Africa, and identify key gaps in data and skills to inform targeted capacity building. Methods: We revised an existing survey, delivered it to 357 participants, then analysed responses and explored key themes. Results: There were 51 respondents (14%) across 14 countries. HTA was considered an important and valuable PS tool with a key role in the design of health benefits packages, clinical guideline development, and service improvement. Medicines were identified as a technology type that would especially benefit from the application of HTA. Using HTA to address safety issues (e.g. low-quality medicines) and value for money concerns was particularly highlighted. The perceived availability and accessibility of suitable local data to support HTA varied widely but was mostly considered inadequate and limited. Respondents also noted a need for training support in research methodology and data gathering. Conclusions: While important in raising awareness of HTA as a tool for PS, this study had a low response rate, and that respondents were self-selected. A more refined survey will be developed to support engagement strategies and capacity building.
Multilocation Newsvendor Problem: Centralization and Inventory Pooling
Authors: Yang Chaolin , Hu Zhenyu , Zhou Sean X. 
Published: May 2020

We study a multilocation newsvendor model with a retailer owning multiple retail stores, each of which is operated by a manager who decides the order quantity for filling random customer demand of a product. Store managers and the retailer are all risk averse, but managers are more risk averse than the retailer. We adopt conditional value-at-risk (CVaR) as the performance measure and consider two alternative strategies to improve the system’s performance. First, the retailer centralizes the ordering decisions. Second, managers still decide the order quantity for their own store, whereas their inventories are pooled together. We analyze and compare the optimal order quantities and the resultant CVaR values of the systems and study their comparative statistics. For centralization, we find that each store has a higher inventory level in the centralized system than in the decentralized system, and centralization positively benefits the retailer as long as some store managers are strictly more risk averse than the retailer. When there is inventory pooling, the ordering decisions in the decentralized system depend on how the additional profit from pooling is allocated among the stores. We consider a weighted proportional allocation rule and characterize the Nash equilibrium of the resultant ordering game among the store managers. Our key finding is that as long as the store managers are sufficiently more risk averse than the retailer or the demands are very heavy tailed, inventory pooling is less beneficial than centralization. We further derive a lower bound on the value of centralization and two upper bounds on the value of inventory pooling. Finally, our analytical results are illustrated using a data set from an online retailer in China, and various comparative statics are further examined via extensive numerical experiments. This paper was accepted by Charles Corbett, operations management.
A CULTURA COMO 'TÁTICA' NAS BATALHAS DE SENTIDO: REFLEXÕES SOBRE AS PRÁTICAS COTIDIANAS DE COLETIVOS CULTURAIS NA CIDADE DE NITERÓI (RJ)
Authors: Castilho Marcio De Souza , Gabry Victor 
Published: May 2020

A comunicação é lugar permanente de disputas e negociações, uma arena em que múltiplos polos de enunciação travam uma luta pelo direito de produzir sentidos no espaço público. O ambiente comunicacional caracterizado pelo impacto da desintermediação reforça a possibilidade de o cidadão produzir outras narrativas, confrontar visões de mundo e estabelecer novos consensos. Nesse sentido, propomos examinar a experiência dos projetos culturais Rap School e Conexão Favela & Arte, situados em Niterói, na Região Metropolitana do Rio de Janeiro, e como estes coletivos estão se apropriando das novas tecnologias de comunicação e informação para pôr em relevo outros atributos sobre o território. Recorrendo a estudos na área da comunicação popular e movimentos sociais, a metodologia também se apóia em entrevistas semi-estruturadas com os realizadores. Como hipótese, sustentamos que os projetos favorecem articulações comunitárias e fortalecem identidades em seus espaços locais.