bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peripheral axonal ensheathment is regulated by RalA GTPase and the exocyst complex
Authors: Silva-Rodrigues Joana F. , Patrício-Rodrigues Cátia F. , de Sousa-Xavier Vicente , Augusto Pedro M. , Fernandes Ana C. , Farinho Ana R. , Martins João P. , Teodoro Rita O. 
Published: January 2020

ABSTRACT
Axon ensheathment is fundamental for fast impulse conduction and the normal physiological functioning of the nervous system. Defects in axonal insulation lead to debilitating conditions, but, despite its importance, the molecular players responsible are poorly defined. Here, we identify RalA GTPase as a key player in axon ensheathment in Drosophila larval peripheral nerves. We demonstrate through genetic analysis that RalA action through the exocyst complex is required in wrapping glial cells to regulate their growth and development. We suggest that the RalA-exocyst pathway controls the targeting of secretory vesicles for membrane growth or for the secretion of a wrapping glia-derived factor that itself regulates growth. In summary, our findings provide a new molecular understanding of the process by which axons are ensheathed in vivo, a process that is crucial for normal neuronal function.
Regulation of embryonic and adult neurogenesis by Ars2
Authors: Yu Yang , Andreu-Agullo Celia , Liu Bing Fang , Barboza Luendreo , Toth Miklos , Lai Eric C. 
Published: January 2020

ABSTRACT
Neural development is controlled at multiple levels to orchestrate appropriate choices of cell fate and differentiation. Although more attention has been paid to the roles of neural-restricted factors, broadly expressed factors can have compelling impacts on tissue-specific development. Here, we describe in vivo conditional knockout analyses of murine Ars2, which has mostly been studied as a general RNA-processing factor in yeast and cultured cells. Ars2 protein expression is regulated during neural lineage progression, and is required for embryonic neural stem cell (NSC) proliferation. In addition, Ars2 null NSCs can still transition into post-mitotic neurons, but fail to undergo terminal differentiation. Similarly, adult-specific deletion of Ars2 compromises hippocampal neurogenesis and results in specific behavioral defects. To broaden evidence for Ars2 as a chromatin regulator in neural development, we generated Ars2 ChIP-seq data. Notably, Ars2 preferentially occupies DNA enhancers in NSCs, where it colocalizes broadly with NSC regulator SOX2. Ars2 association with chromatin is markedly reduced following NSC differentiation. Altogether, Ars2 is an essential neural regulator that interacts dynamically with DNA and controls neural lineage development.
Do you cov me? Effect of coverage reduction on metagenome shotgun sequencing studies
Authors: Cattonaro Federica , Spadotto Alessandro , Radovic Slobodanka , Marroni Fabio 
Published: January 2020

Shotgun metagenomics sequencing is a powerful tool for the characterization of complex biological matrices, enabling analysis of prokaryotic and eukaryotic organisms and viruses in a single experiment, with the possibility of reconstructing de novo the whole metagenome or a set of genes of interest. One of the main factors limiting the use of shotgun metagenomics on wide scale projects is the high cost associated with the approach. We set out to determine if it is possible to use shallow shotgun metagenomics to characterize complex biological matrices while reducing costs. We used a staggered mock community to estimate the optimal threshold for species detection. We measured the variation of several summary statistics simulating a decrease in sequencing depth by randomly subsampling a number of reads. The main statistics that were compared are diversity estimates, species abundance, and ability of reconstructing de novo the metagenome in terms of length and completeness. Our results show that diversity indices of complex prokaryotic, eukaryotic and viral communities can be accurately estimated with 500,000 reads or less, although particularly complex samples may require 1,000,000 reads. On the contrary, any task involving the reconstruction of the metagenome performed poorly, even with the largest simulated subsample (1,000,000 reads). The length of the reconstructed assembly was smaller than the length obtained with the full dataset, and the proportion of conserved genes that were identified in the meta-genome was drastically reduced compared to the full sample. Shallow shotgun metagenomics can be a useful tool to describe the structure of complex matrices, but it is not adequate to reconstruct—even partially—the metagenome.
Análisis de datos para el marketing digital emprendedor: Caso de estudio Parque de Innovación Empresarial - Universidad Nacional sede Manizales
Authors: Marín López Juan Camilo , López Trujillo Marcelo 
Published: January 2020

El marketing digital o e-marketing ofrece diferentes oportunidades y beneficios que pueden ser aprovechadas por las pymes para mejorar su eficiencia y competitividad. Sin embargo, el desafío real es analizar los datos recopiladosy utilizarlos para optimizar procesos y recursos. A pesar de la considerable importancia que se le ha dado a este tema, se han realizado pocas investigaciones que unan los conceptos de e-marketing y análisis de datos en el contextoemprendedor. Este estudio se propuso explorar y describir el panorama del análisis de datos para el marketing digital en el Parque de Innovación Empresarial de Manizales, mediante la realización de 20 encuestas. En esta exploración,se encontró que los microempresarios reconocen la importancia y utilizan el marketing digital, pero solo la mitad de ellos realiza un análisis de datos superficial o básico, debido a diversas limitaciones que se expondrán. Se abren nuevas puertas para futuros estudios que tengan un enfoque de choque frente a la realidad encontrada.
Effective multidisciplinary approach for treatment of traumatized maxillary incisors and hypodontia
Authors: Elhussein Mustafa , O'Dwyer Jonathan J , Sandler Jonathan 
Published: January 2020

Fractured and missing teeth within the aesthetic zone have always posed a clinical challenge for the dental team. Management and treatment requires the input from a number of dental specialists to guarantee a high quality result. A case report is presented to demonstrate an effective combined orthodontic/restorative approach that fully restored aesthetics and function. The report is of a 14-year-old girl who traumatized her maxillary central incisors, in a dentition that was already compromised as she had congenital absence of her maxillary left lateral incisor. Using fixed orthodontic appliances, the fractured teeth were extruded to bring the fracture line above the level of the alveolar bone. This movement allowed an immediate aesthetic improvement, using stainless steel prefabricated posts and composite resin buildups, which also facilitated further orthodontic tooth movement. Teeth, that would otherwise have been extracted, were salvaged and, utilizing a multidisciplinary team approach, a reasonable long-term prognosis has resulted. CPD/Clinical Relevance: This case report aims to demonstrate how a multidisciplinary team approach in Orthodontics can be utilized for management of severely handicapped dentitions that have sustained dental injury. It is not the aim of this case report to discuss the treatment of dental injuries but to demonstrate and provide an overview of the possible treatment modalities that can be effective in restoring function and aesthetics, with special consideration given to missing teeth and subgingival crown fractures within the aesthetic zone.
Compact efficient high-power triple-color Nd:YVO4 yellow-lime-green self-Raman lasers
Authors: Liu Y. C. , Chen C. M. , Hsiao J. Q. , Pan Y. Y. , Tsou C. H. , Liang H. C. , Chen Y. F. 
Published: January 2020

A novel, to the best of our knowledge, approach is developed to realize a high-power compact efficient yellow-lime-green triple-color

N
d

:

Y
V
O

4

self-Raman laser. The 588 nm yellow laser, the 559 nm lime laser, and the 532 nm green laser are converted from the 1064 nm fundamental wave and the 1176 nm Stokes Raman field. The simultaneous three-color operation is accomplished with three stages to step-by-step generate the 588 nm, 559 nm, and 532 nm lasers by using three different lithium triborate (LBO) crystals. By tuning the temperature of each individual LBO crystal, the 588 nm, 559 nm, and 532 nm output powers can be nearly the same and concurrently up to 2.4 W at the incident pump power of 30 W, corresponding to a conversion efficiency of 24% for the total output power.
Broadband on-chip photonic spin Hall element via inverse design
Authors: Xie Zhenwei , Lei Ting , Qiu Haodong , Zhang Zecen , Wang Hong , Yuan Xiaocong 
Published: January 2020

The photonic spin Hall effect plays an important role in photonic information technologies, especially in on-chip spin Hall devices. However, conventional devices suffer from low efficiency or narrow bandwidth, which prevents their practical application. Here, we introduce a spin Hall device using inverse design to achieve both high efficiency and broadband. Spin-dependent light separation is enabled by a 2.4 μm circular device with 100 nm pixels. The photonic spin Hall element is fabricated on a silicon-on-insulator wafer compatible with a standard integrated photonic circuit. The spin light is detected and emitted with an efficiency of up to 22% and 35%, respectively, over a 200 nm bandwidth at optical wavelength.
Erbium-doped TeO2-coated Si3N4 waveguide amplifiers with 5  dB net gain
Authors: Frankis Henry C. , Mbonde Hamidu M. , Bonneville Dawson B. , Zhang Chenglin , Mateman Richard , Leinse Arne , Bradley Jonathan D. B. 
Published: January 2020

We demonstrate 5 dB net gain in an erbium-doped tellurium-oxide-coated silicon nitride waveguide. The amplifier design leverages the high refractive index and high gain in erbium-doped tellurite glass as well as the ultra-low losses and mature, reliable, and low-cost fabrication methods of silicon nitride waveguide technology. We show that the waveguide platform demonstrates low background propagation losses of 0.25 dB/cm based on a ring resonator device with a

Q

factor of

1.3
×

10
6

at 1640 nm. We measure 5 dB peak net gain at 1558 nm and

>
3

dB

of net gain across the C band in a 6.7 cm long waveguide for 35 mW of launched 1470 nm pump power. Gain per unit length of 1.7 and 1.4 dB/cm is measured in a 2.2 cm long waveguide for 970 and 1470 nm pump wavelengths, respectively. Amplifier simulations predict that

>
10

dB

gain can be achieved across the C band simply by optimizing waveguide length and fiber-chip coupling. These results demonstrate a promising approach for the monolithic integration of compact erbium-doped waveguide amplifiers on silicon nitride chips and within silicon-based photonic integrated circuits.
Comparison of nutritional status among children with biliary atresia according to age at the time of Kasai procedure
Authors: Pratiwi Febricilla Citra , Widowati Titis , Prawirohartono Endy Paryanto 
Published: January 2020

Background Recent studies revealed better outcomes among children with biliary atresia (BA) who underwent Kasai procedure at 60 to 90 days of age. Delayed Kasai procedure has a high risk of complications, including nutritional deficits which lead to malnutrition.
Objective To determine the nutritional status of children with BA according to age at the time of Kasai procedure.
Methods Using medical records, we conducted a retrospective study in children with BA based on magnetic resonance cholangiopancreatography (MRCP) or intraoperative cholangiography, who were admitted between 2015 to 2017 and underwent Kasai surgery at Dr. Sardjito General Hospital, Yogyakarta. Nutritional status was evaluated on the day before Kasai procedure, and classified into 4 groups of subjects based on age at the time the children underwent Kasai procedure (<60 days, 60-90 days, >90-120 days, and >120 days). Normal distribution data was analyzed with Saphiro-Wilk test and mean T-test was used to compare mean age at the time of Kasai procedure between groups of well-nourished and malnourished subjects.
Results A total of 39 children with BA underwent Kasai procedure. Of 3 children who underwent Kasai procedure at <60 days of age, 2 were well-nourished and 1 was malnourished. Of the 12 children who underwent Kasai procedure at 60-90 days of age, 6 were well-nourished and 6 were malnourished. Of the 7 children who underwent Kasai procedure at >90-120 days of age, 4 were well-nourished and 3 were malnourished. Of 17 children who underwent Kasai procedure at >120 days of age, 5 were well-nourished and 12 were malnourished. The means of age at the time of Kasai procedure were higher in malnourished subject than well-nourished.
Conclusion The highest prevalence of malnourishment is seen in children with biliary atresia who underwent Kasai procedure at >120 days of age.
Process planning and scheduling optimisation with alternative recipes
Authors: Dziurzanski Piotr , Zhao Shuai , Scholze Sebastian , Zilverberg Albert , Krone Karl , Indrusiak Leandro Soares 
Published: January 2020

Abstract
This paper considers an application of a new variant of a multi-objective flexible job-shop scheduling problem, featuring multisubset selection of manufactured recipes, to a real-world chemical plant. The problem is optimised using a multi-objective genetic algorithm with customised mutation and elitism operators that minimises both the total production time and the produced commodity surplus. The algorithm evaluation is performed with both random and historic manufacturing orders. The latter demonstrated that the proposed system can lead to more than 10 % makespan improvements in comparison with human operators.
Protective effects of emodin on lung injuries in rat models of liver fibrosis
Authors: Zhang Lili , Zhang Huiying , Wang Limin , Fan Yimin , Zhang Cuiying , Li Xujiong , Han Dewu , Ji Cheng 
Published: January 2020

AbstractObjectiveThe aim of this study is to investigate the protective effects of emodin (EMD) on the lung injuries in the rat models of liver fibrosis.MethodsLiver fibrosis was established in rats and the effect of intervention using EMD treatment was determined. Liver and lung weight coefficients were measured and lung content of TNF-α (tumor necrosis factor α), MDA (malondialdehyde), NO (nitric oxide), and ONOO- (peroxynitrite) were determined. Finally, histopathological changes were evaluated.ResultsCompared with the normal control group, the lung weight coefficient was significantly increased in the fibrosis model group. Moreover, pulmonary edema and inflammatory responses were observed. Levels of TNF-α, MDA, NO, and ONOO- in the lung homogenate were significantly increased in the fibrosis model group. After EMD treatment, the lung weight coefficients were significantly reduced. Moreover, pathological changes in the lung tissue were dramatically alleviated. Levels of TNF-α, MDA, NO, and ONOO- were significantly decreased.ConclusionEMD exhibits protective effects against lung injuries in a rat model of liver fibrosis.
Violin varnish induced changes in the vibro-mechanical properties of spruce and maple wood
Authors: Lämmlein Sarah Louise , Van Damme Bart , Mannes David , Schwarze Francis Willis Matthew Robert , Burgert Ingo 
Published: January 2020

Abstract
Violin varnishes are known to alter the longitudinal and radial material properties of violin tonewood. Here, the varnish-induced changes in the vibrational and elastic properties of spruce and maple wood were studied by modal analysis of rectangular plates. This contact-free investigation yields the eigenfrequencies and corresponding mode shapes and modal damping ratios of multiple out-of-plane modes. The longitudinal and radial E-modulus and the in-plane shear modulus were determined by an inverse material property determination process. The influence of traditional European varnish materials and different coating build-ups, comprising alcohol-based as well as oil-based varnishes, was studied during their application and for several months during drying and aging of the multi-layered systems. The evolutions of the changes induced by the different varnishes were rather similar and, except for modal damping, less pronounced for maple than for spruce. For the latter, the longitudinal E-modulus was slightly decreased while the shear modulus and radial E-modulus were increased after 18 months. The strong increases in modal damping ratios shortly after applying the coating systems (up to 150%) were reduced over time but remained significant for the time-span studied. However, also small differences between the influence of different coating systems and varnish materials on the amplitude of the induced changes became apparent.
Timber moisture content adjustment factors for nondestructive testing (NDT): acoustic, vibration and probing techniques
Authors: Llana Daniel F. , Íñiguez-González Guillermo , Esteban Miguel , Hermoso Eva , Arriaga Francisco 
Published: January 2020

AbstractNondestructive testing (NDT) includes several highly efficient techniques for the estimation of the physical and mechanical properties of structural timber. However, NDT results are affected by several factors [moisture content (MC), temperature, specimen dimensions, sensor positioning and grain angle, timber-sensor coupling, etc.], of which wood MC is the most important. Scientific research on MC influence in NDT measurements started in the 19thcentury, and 2018 was the first year that a MC adjustment for NDT measurements was published in a European standard (EN14081-2). Although MC influence on NDT has been studied for more than 170 years, MC adjustment factor research is important nowadays because it is widely used in the industry. Currently, NDT device manufacturers develop their own MC adjustment factors adapted to their equipment and species. This paper presents an exhaustive review of MC adjustments for different types of NDT equipment, as proposed by several authors from the 19thcentury to date, and a discussion of factors affecting MC adjustments.
Changes in the wood-water interactions of mahogany wood due to heat treatment
Authors: Zhou Fan , Fu Zongying , Gao Xin , Zhou Yongdong 
Published: January 2020

Abstract
Mahogany wood (Swietenia macrophylla King) was thermally modified by heating at various temperatures. The wood-water-related parameters of the heat-treated wood, including fiber saturation point, equilibrium moisture content, moisture excluding efficiency, hygroscopic hysteresis, swelling, anti-swelling efficiency, water adsorption, and surface wettability were determined to clarify the mechanism of heat treatment to reduce wood hygroscopicity. The wood treated at a higher temperature demonstrated a more significant decrease in hygroscopicity. The reduction in hygroscopicity of the heat-treated wood was partially diminished as the moisture excluding efficiency decreased in the absorption and desorption processes caused by the changes in environmental temperature and relative humidity. The fiber saturation point (determined by nuclear magnetic resonance spectroscopy), surface free energy, and the surface wettability of wood were reduced by heat treatment, resulting in the decreased hygroscopicity of the heat-treated wood. Mahogany wood became more insensitive to the influence of moisture due to the heat treatment, and this effect was more distinct for wood treated at a high temperature.
Asymmetric inheritance of mitochondria in yeast
Authors: Klecker Till , Westermann Benedikt 
Published: January 2020

Abstract
Mitochondria are essential organelles of virtually all eukaryotic organisms. As they cannot be made de novo, they have to be inherited during cell division. In this review, we provide an overview on mitochondrial inheritance in Saccharomyces cerevisiae, a powerful model organism to study asymmetric cell division. Several processes have to be coordinated during mitochondrial inheritance: mitochondrial transport along the actin cytoskeleton into the emerging bud is powered by a myosin motor protein; cell cortex anchors retain a critical fraction of mitochondria in the mother cell and bud to ensure proper partitioning; and the quantity of mitochondria inherited by the bud is controlled during cell cycle progression. Asymmetric division of yeast cells produces rejuvenated daughter cells and aging mother cells that die after a finite number of cell divisions. We highlight the critical role of mitochondria in this process and discuss how asymmetric mitochondrial partitioning and cellular aging are connected.
Antidiabetic and protective effects of Scrophularia striata ethanolic extract on diabetic nephropathy via suppression of RAGE and S100A8 expression in kidney tissues of streptozotocin-induced diabetic rats
Authors: Alaee Mona , Akbari Ahmad , Karami Hadi , Salemi Zahra , Amri Jamal , Panahi Mitra 
Published: January 2020

Abstract

Background
The present study was conducted to examine the antidiabetic effects of Scrophularia striata ethanolic extract and to evaluate its effects on oxidative stress markers and RAGE and S100A8 gene expressions in the kidney of type 1 diabetic rats.

Methods
A total of 36 rats (weight 200–250 g) were randomly assigned into six groups as follows: Cnt, Cnt + S. striata 100, and Cnt + S. striata 200 that received normal saline, 100 mg/kg bw, and 200 mg/kg bw of ethanol extract of S. striata, respectively; and group Dibt, Dibt + S. striata 100, and Dibt + S. striata 200 that received normal saline, 100 mg/kg bw, and 200 mg/kg bw of ethanol extract of S. striata, respectively. Type 1 diabetes was induced in rats by a single injection of streptozotocin (55 mg/kg bw). After 60 days of treatment, biochemical factors and oxidative stress markers (superoxide dismutase [SOD] and malondialdehyde [MDA]) were measured using spectrophotometric methods. RAGE and S100A8 gene expressions were analyzed using real-time polymerase chain reaction.

Results
Diabetes significantly impairs serum and urine fasting blood glucose (FBG), lipid profile, creatinine, urea, and albumin parameters. After the treatment with S. striata extract, these parameters are close to the normal range. It was shown that the S. striata extract significantly decreased the kidney expression levels of RAGE and S100A8 genes and improved oxidative stress markers (SOD and MDA) in the kidney tissues when compared with the diabetic control group. It was also found that the beneficial effects of the S. striata were dose dependent.

Conclusions
The ethanolic extract of S. striata has beneficial antidiabetic effects. Moreover, by reducing RAGE and S100A8 gene expressions and by improving oxidative stress, S. striata might be used as adjuvant treatment for diabetic complications.