bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantifying Molecule Numbers in STED/RESOLFT Fluorescence Nanoscopy
Authors: Keller-Findeisen Jan , Sahl Steffen J. , Hell Stefan W. 
Published: June 2020

AbstractQuantification of the numbers of molecules of interest in the specimen has emerged as a powerful capability of several fluorescence nanoscopy approaches. Carefully relating the measured signals from STED or RESOLFT scanning nanoscopy data to the contribution of a single molecule, reliable estimates of fluorescent molecule numbers can be obtained. To achieve this, higher-order signatures in the obtained photon statistics are analyzed, as arise from the antibunched nature of single-fluorophore emissions or in the signal variance among multiple on/off-switching cycles. In this chapter, we discuss the concepts and approaches demonstrated to date for counting molecules in STED/RESOLFT nanoscopy.
Healing Mainstream Health: Building Understanding and Respect for Indigenous Knowledges
Authors: Rix Liz , Rotumah Darlene 
Published: June 2020

AbstractWe first encountered one another around a decade ago when sharing an office at a rural health campus of The University of Sydney, where both of us were working on health research projects. From that first meeting we were intuitively drawn to each other, and soon discovered shared interests, and some surprisingly common perspectives and opinions on health services, seen with an Indigenous Australian lens.
The Sydney Language on Our Campuses and in Our Curriculum
Authors: Quinnell Rosanne , Troy Jakelin , Poll Matthew 
Published: June 2020

AbstractNow my motivations … so, my aspirations for all of this is of course first and foremost, I want to learn my own language again, I want to have that knowledge point. People ask me how to speak, “do you speak Aboriginal?” I feel like that’s a really weird question, but yes, I can. Second of all, I want to bring that back to my family.
Students and Academics Working in Partnership to Embed Cultural Competence as a Graduate Quality
Authors: Bell Amani , Barahona Stephanie , Beg Gulnaz , Coulson Susan , Eymont Roman , Hartman Jodie , Hubble Tom , Leung Natalie , McDonnell Michael A. , Ni Jiaru , Peseta Tai , Sakhaee Ehssan , Uptin Jonnell 
Published: June 2020

AbstractSince 2014, the University of Sydney has been experimenting with a new initiative motivated by the research on “students as partners”. In 2014, six students were selected as Ambassadors of the Sydney Teaching ColloquiumSydney Teaching Colloquium (STC)—the University’s annual learning and teaching conference—as undergraduate researchers. In that year, the focus was on assessment standards.
Embedding Cultural Competence in Science Curricula
Authors: Cross Rebecca , Bone Elisa , Ampt Peter , Bell Tina , Quinnell Rosanne , Gongora Jaime 
Published: June 2020

AbstractEffectively incorporating cultural competence into tertiary institutions is paramount to the creation of cross-cultural settings where undergraduates and academics can develop understandings of how culture and belief systems influence professional decision making. Processes that incorporate cultural competence are viewed as particularly challenging in science disciplines, particularly non-vocational science disciplines where “western” or reductivist ways of teaching and “doing” science remain dominant.
Learning Through Reflection—Enhancing Culturally Proficient Learning Communities in Midwifery Practice and Education: An Experience-Based Learning Journey in London, UK
Authors: Haora Penny 
Published: June 2020

AbstractThis reflective narrative shares an experience of immersion at the highly political “intersectional” coalface of gendered, class and racially based maternity services research, practice and learning in London, UK. I initially set out to apply Levin & Greenwoods’ (Handbook of action research: participative inquiry and practice. SAGE, London, 2001, p. 103) “pragmatic action research” approach to transform a small component of universities and health services into “learning communities”.
Future Directions: Cultural Competence and the Higher Education Sector
Authors: Frawley Jack , Russell Gabrielle , Sherwood Juanita 
Published: June 2020

AbstractIn one sense or another, the chapters in this book have all acknowledged that cultural competence is a set of behaviours, attitudes and/or policies that come together in the higher education sector or among professional and academic staff, and students enabling them to operate efficiently in intercultural contexts (Cross, Bazron, Dennis, & Isaacs in Towards a culturally competent system of care: a monograph on effective services for minority children who are severely emotionally disturbed. Child and Adolescent Service System Program Technical Assistance Center, Georgetown University Child Development Center, Washington, DC, 1989).
Beliefs, Events and Values Inventory Assessment of Global Identity: Implications and Applications for International, Cross-Cultural and Transformative Learning
Authors: Wang Felix , Pait Kathleen , Acheson Kris , Sternberger Lee , Staton Renee , Shealy Craig N. 
Published: June 2020

AbstractAs global communities become more intertwined both technologically and socially, professionals in every field are expected to work with people from different backgrounds and thrive in multicultural settings.
Evaluating Cultural Competence in Indigenous Higher Education Contexts in Australia: A Challenge for Change
Authors: Smith James A. , Robertson Kim 
Published: June 2020

AbstractIndigenous participation and achievement in education is an issue of national and international significance. Within Australia, theReview of Higher Education Access and Outcomes for Aboriginal and Torres Strait Islander PeopleReview of Higher Education Access and Outcomes for Aboriginal and Torres Strait Islander People—often referred to as the Behrendt Review—provided a clear blueprint for investing in policies and programmes that support Indigenous pathways, transitions, participation and achievement in higher education.
FGFR-gerichtete Therapie von Kopf-Hals-Karzinomen
Authors: Dietrich Dimo 
Published: June 2020

ZusammenfassungHintergrundGenomische Veränderungen (Mutationen, Genfusionen, Amplifikationen) und eine Dysregulierung des Fibroblasten-Wachstumsfaktor-Rezeptor-Signalwegs („fibroblast growth factor (FGF) receptor“, FGFR) sind häufig in Plattenepithelkarzinomen des Kopf-Hals-Bereichs (HNSCC) zu finden. Eine zielgerichtete Therapie mit gegen FGF-Rezeptoren gerichteten Tyrosinkinaseinhibitoren (TKI) oder monoklonalen Antikörpern stellt daher einen vielversprechenden Ansatz für die Behandlung des HNSCC dar.FragestellungDieser Übersichtsartikel beschreibt den aktuellen Wissensstand hinsichtlich FGFR-gerichteter Therapien bei Kopf-Hals-Tumoren (insbesondere HNSCC) und diskutiert in diesem Kontext genomische Veränderungen des FGFR-Signalwegs als potenzielle begleitende prädiktive Biomarker.Material und MethodeDer vorliegende Artikel basiert auf einer Recherche der Datenbanken PubMed, ClinicalTrials.gov sowie von Tagungsbänden.ErgebnisseErste Ergebnisse belegen die Wirksamkeit von Tyrosinkinase-Inhibitoren sowohl bei HNSCC als auch bei Adenokarzinomen des Kopf-Hals-Bereichs, insbesondere bei Schilddrüsenkarzinomen und adenoid-zystischen Speicheldrüsenkarzinomen.SchlussfolgerungenFrühe klinische und präklinische Daten verdeutlichen das Potenzial einer biomarkergesteuerten Behandlung von Patienten mit Kopf-Hals-Tumoren mit gegen FGFR-gerichtete TKI.
Multi-parent multi-environment QTL analysis: an illustration with the EU-NAM Flint population
Authors: Garin Vincent , Malosetti Marcos , van Eeuwijk Fred 
Published: June 2020

Abstract
Key message
Multi-parent populations multi-environment QTL experiments data should be analysed jointly to estimate the QTL effect variation within the population and between environments.

Abstract
Commonly, QTL detection in multi-parent populations (MPPs) data measured in multiple environments (ME) is done by analyzing genotypic values ‘averaged’ across environments. This method ignores the environment-specific QTL (QTLxE) effects. Running separate single environment analyses is a possibility to measure QTLxE effects, but those analyses do not model the genetic covariance due to the use of the same genotype in different environments. In this paper, we propose methods to analyse MPP-ME QTL experiments using simultaneously the data from several environments and modelling the genotypic covariance. Using data from the EU-NAM Flint population, we show that these methods estimate the QTLxE effects and that they can improve the quality of the QTL detection. Those methods also have a larger inference power. For example, they can be extended to integrate environmental indices like temperature or precipitation to better understand the mechanisms behind the QTLxE effects. Therefore, our methodology allows the exploitation of the full MPP-ME data potential: to estimate QTL effect variation (a) within the MPP between sub-populations due to different genetic backgrounds and (b) between environments.
Ultrasound-guided muscle biopsy: a practical alternative for investigation of myopathy
Authors: Raithatha Anish , Ashraghi Mohammad Reza , Lord Christopher , Limback-Stanic Clara , Viegas Stuart , Amiras Dimitri 
Published: June 2020

Abstract
Objective
We propose the use of ultrasound-guided muscle biopsy as a viable method of obtaining muscle specimen to aid the diagnosis of myopathy. We retrospectively review the diagnostic accuracy and patient feedback of ultrasound-guided muscle biopsies in our neuromuscular service.

Method
Multidisciplinary team meeting reviewed select patients and agreed on those suitable for ultrasound-guided muscle biopsy. They then underwent biopsy using direct ultrasound guidance and a modified Bergström needle. The specimens were sent for histopathological analysis, and patients were given a feedback form.

Results
Ten patients underwent 11 ultrasound-guided muscle biopsies. Of these 11, one was processed incorrectly, but all others were good quality specimens suitable for analysis. All 10 of those processed correctly aided diagnosis. All patient feedback was rated good or excellent. In 4 patients with a previous unsuccessful surgical biopsy, ultrasound-guided biopsy was successful in obtaining suitable muscle. Of those 4 patients, 3 preferred ultrasound-guided biopsy, and 1 did not state a preference.

Discussion
Our ultrasound-guided muscle biopsy technique offers a viable alternative to surgical biopsy. It yields high-quality specimen that aids diagnosis and receives good feedback from patients. It can be performed quickly as a day case and does not require theatre space. Furthermore, direct visualization of structures minimizes the risk of complications and allows biopsy of otherwise difficult to access targets.

Conclusion
Utilization of ultrasound guided–modified Bergström needle technique for muscle biopsy provides comparable success rates to other techniques and has practical, clinical, operational, and patient-centred benefits compared with alternative techniques.
Measuring the cellular memory B cell response after vaccination in patients after allogeneic stem cell transplantation
Authors: Winkler Julia , Tittlbach Hannes , Schneider Andrea , Buchstaller Corinna , Mayr Andreas , Vasova Ingrid , Roesler Wolf , Mach Michael , Mackensen Andreas , Winkler Thomas H. 
Published: June 2020

AbstractAfter allogeneic hematopoietic stem cell transplantation (HSCT), patients are repetitively vaccinated to reduce the risk of infection caused by the immune deficiency following allogeneic HSCT. By the vaccination of transplanted patients, the humoral memory function can be restored in the majority of cases. It is unknown, however, to what extent memory B cells derived from the donor contribute to the mobilization of antibody-secreting cells and long-term humoral memory in patients after allogeneic HSCT. We therefore analyzed patients after allogeneic HSCT for memory B cell responses 7 days after single vaccination against tetanus toxoid (TT), diphtheria toxoid (DT), pertussis toxoid (PT),Haemophilus influenzaetype b (Hib), and poliovirus. Patients showed an insufficient mobilization of plasmablasts (PB) after vaccination, whereas healthy subjects (HD,n= 13) exhibited a significant increase of PB in the peripheral blood. Regarding vaccine-specific antibody-secreting PB, all HD responded against all vaccine antigens, as expected. However, only 65% of the patients responded with a measurable increase in IgG-secreting PB against TT, 65% against DT, 33% against PT, and 53% against poliovirus. Correspondingly, the antibody titers on day 7 after vaccination did not increase in patients. A significant increase of serum titers for the vaccine antigens was detectable in the majority of patients only after repetitive vaccinations. In contrast to the low mobilization of vaccine-specific PB after vaccination, a high number of PB before vaccination was detectable in patients following allogeneic HSCT. High frequencies of circulating PB correlated with the incidence of moderate/severe chronic GVHD. In summary, patients showed a weak mobilization of antigen-specific PB and an inadequate increase in antibody titers 7 days after the first vaccination. Patients with moderate or severe chronic GVHD in their history had a significantly higher percentage of IgG-secreting PB prior to vaccination. The antigen specificity of these IgG-secreting PB is currently unknown.
Estimation of PM2.5 Mass Concentration from Visibility
Authors: Ji Denghui , Deng Zhaoze , Sun Xiaoyu , Ran Liang , Xia Xiangao , Fu Disong , Song Zijue , Wang Pucai , Wu Yunfei , Tian Ping , Huang Mengyu 
Published: June 2020

AbstractAerosols in the atmosphere not only degrade visibility, but are also detrimental to human health and transportation. In order to develop a method to estimate PM2.5 mass concentration from the widely measured visibility, a field campaign was conducted in Southwest China in January 2019. Visibility, ambient relative humidity (RH), PM2.5 mass concentrations and scattering coefficients of dry particles were measured. During the campaign, two pollution episodes, i.e., from 4–9 January and from 10–16 January, were encountered. Each of the two episodes could be divided into two periods. High aerosol hygroscopicity was found during the first period, when RH was higher than 80% at most of the time, and sometimes even approached 100%. The second period experienced a relatively dry but more polluted condition and aerosol hygroscopicity was lower than that during the first period. An empirical relationship between PM2.5 mass concentration and visibility (ambient aerosol extinction) under different RH conditions could thus be established. Based on the empirical relationship, PM2.5 mass concentration could be well estimated from visibility and RH. This method will be useful for remote sensing of PM2.5 mass concentration.
Obstructive sleep apnoea but not central sleep apnoea is associated with left ventricular remodelling after acute myocardial infarction
Authors: Fisser Christoph , Götz Kristina , Hetzenecker Andrea , Debl Kurt , Zeman Florian , Hamer Okka W. , Poschenrieder Florian , Fellner Claudia , Stadler Stefan , Maier Lars S. , Pfeifer Michael , Buchner Stefan , Arzt Michael 
Published: June 2020

Abstract
Obejctive
Obstructive sleep apnoea (OSA) increases left ventricular transmural pressure more than central sleep apnoea (CSA) owing to negative intrathoracic pressure swings. We tested the hypothesis that the severity of OSA, and not CSA, is therefore associated with spheric cardiac remodelling after acute myocardial infarction.

Methods
This sub-analysis of a prospective observational study included 24 patients with acute myocardial infarction who underwent primary percutaneous coronary intervention. Spheric remodelling, calculated according to the sphericity index, was assessed by cardiac magnetic resonance imaging at baseline and 12 weeks after acute myocardial infarction. OSA and CSA [apnoea-hypopnoea index (AHI) ≥ 5/hour] were diagnosed by polysomnography.

Results
Within 12 weeks after acute myocardial infarction, patients with OSA exhibited a significant increase in systolic sphericity index compared to patients without sleep-disordered breathing (no SDB) and patients with CSA (OSA vs. CSA vs. no SDB: 0.05 ± 0.04 vs. 0.01 ± 0.04 vs. − 0.03 ± 0.03, p = 0.002). In contrast to CSA, the severity of OSA was associated with an increase in systolic sphericity index after accounting for TIMI-flow before percutaneous coronary intervention, infarct size, pain-to-balloon-time and systolic blood pressure [OSA: B (95% CI) 0.443 (0.021; 0.816), p = 0.040; CSA: 0.193 (− 0.134; 0.300), p = 0.385].

Conclusion
In contrast to CSA and no SDB, OSA is associated with spheric cardiac remodelling within the first 12 weeks after acute myocardial infarction. Data suggest that OSA-related negative intrathoracic pressure swings may contribute to this remodelling after acute myocardial infaction.

Graphic abstract
Efficient propagation of suspended HL-60 cells in a disposable bioreactor supporting wave-induced agitation at various Reynolds number
Authors: Wierzchowski Kamil , Grabowska Iwona , Pilarek Maciej 
Published: June 2020

AbstractGrowth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions of 2-D wave-assisted agitation has been compared and discussed. Influence of Reynolds number for liquid (ReL) and the kLa coefficient, as key parameters characterized the bioprocessing of HL-60 cells in ReadyToProcess WAVETM 25 system, on reached values of the apparent maximal specific growth rate (μmax) and the specific yield of biomass (Y*X/S) has been identified. The values of ReL (i.e., 510–10,208), as well as kLa coefficient (i.e., 2.83–13.55 h−1), have been estimated for the cultures subjected to wave-induced mixing, based on simplified dimensionless correlation for various presents of WAVE 25 system. The highest values of apparent μmax = 0.038 h−1 and Y*X/S = 25.64 × 108 cells gglc−1 have been noted for cultures independently performed at wave-induced agitation characterized by ReL equaled to 5104 and 510, respectively. The presented results have high applicability potential in scale-up of bioprocesses focused on nonadherent animal cells, or in the case of any application of disposable bioreactors presenting similitude.
Dating of detrital zircons and tracing the provenance of quartzites from the Bystrzyckie Mts: implications for the tectonic setting of the Early Palaeozoic sedimentary basin developed on the Gondwana margin
Authors: Szczepański Jacek , Turniak Krzysztof , Anczkiewicz Robert , Gleichner Paulina 
Published: June 2020

AbstractWe studied the petrographical characteristics and bulk chemical composition along with the age of detrital zircon grains from quartzites exposed in the western part of the Orlica-Śnieżnik dome in the Saxothuringian zone. Age spectra of detrital zircons were obtained by U–Pb laser ablation inductively coupled plasma mass spectrometry dating. The zircon ages define a few clusters of which the most numerous are Cambro-Ordovician and Neoproterozoic, and less frequent are Paleoproterozoic and Archean. A distinctive feature of the investigated samples is the lack of Mesoproterozoic zircon grains. The youngest age cluster documented in the investigated quartzites points to a Late Cambrian maximum depositional age and detritus derivation from erosion of Cambro-Ordovician metagranitoids. Bulk chemical composition of the quartzites indicates that they originated owing to erosion of granite within sedimentary basins developed on a passive continental margin. Presented data prove the similarity of the examined rocks to quartzites known from the eastern part of the Orlica-Śnieżnik dome and quartz metasandstones documented in other parts of the Variscan Belt of Europe known as the Armorican quartzites or their equivalents exposed in the Saxothuringian zone. Our results support the suggestion that the Orlica-Śnieżnik dome has an affinity to the West African Craton of the Gondwana margin and represents a fragment of the Saxothuringian zone.
A health economic analysis of the management of open lower limb fractures in the elderly
Authors: Pley Caitlin , Purohit Katie , Krkovic Matija , Abdulkarim Ali 
Published: June 2020

Abstract
Aim
The aim of this study was to investigate the financial implications of the inpatient management of open lower limb fractures in adults over 65 years old. Further, the study compares the calculated cost to the income received by the hospital for these patients and to the existing body of literature.

Methods
This study employed direct inpatient costing analysis to estimate the cost of treating the open lower limb fractures incurred by 58 patients over the age of 65 years treated in our centre (Addenbrooke's Hospital, Cambridge University Hospitals NHS Trust) between March 2014 and March 2019.

Results
The median cost of inpatient care calculated in this study was £20,398 per patient, resulting in a financial loss to the hospital of £5113 per patient. When the results were disaggregated by sex, the median cost for an open lower limb fracture in a male patient was £20,886 compared to £19,304 in a female patient. Data were also disaggregated by the site of injury, which produced a median cost for an open femur fracture of £23,949, and £24,549 and £15,362 for open tibia and ankle fractures, respectively.

Conclusion
This study provides a valuable estimate of the expense of treating open lower limb fractures in patients over the age of 65 years in a Major Trauma Centre in England. The study highlights the large losses incurred by hospitals in treating these cases, and supports revision of the remuneration structures in the National Health Service to adequately cover their cost.
Evaluation of low-viscosity bulk-fill composites regarding marginal and internal adaptation
Authors: Park Kyung-Jin , Pfeffer Manon , Näke Thomas , Schneider Hartmut , Ziebolz Dirk , Haak Rainer 
Published: June 2020

AbstractThis study aimed at evaluating the marginal and internal adaptation of low-viscosity bulk-fill composites to enamel and dentin using a self-etch or an etch-and-rinse adhesive without and with artificial ageing. Hundred and twenty-eight MOD cavities in extracted molars were assigned to eight groups (n = 16), restored with the adhesives OptiBond FL (OFL) or Xeno V+ (X) and two low-viscosity bulk-fill composites SDR or x-tra base, covered with Premise. Tetric EvoCeram Bulk Fill and Premise served as a control.n = 8 per group were subjected to prolonged water storage (180 days) and thermocycling (2500×). Scanning electron microscopy was used to determine marginal gaps (MG) and interfacial adhesive defects (IAD). There were no significant differences between composite types in 44 out of 48 (MG) or 43/48 (IAD) comparisons. More MG were observed with X than with OFL (14 out of 16 comparisons, two significant), while in 16 of 16 comparisons with X more IAD were observed (14 significant). After artificial ageing, MG generally increased (9/16 significant), compared to IAD (one significant). The performance of the investigated composite types concerning the integrity of the tooth-composites interface was comparable. Compared to the 1-step self-etch system, the bond with the 3-step etch-and-rinse adhesive was raised.