bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
Authors: Mukherjee Debasmita , DiVincenzo Mallory J. , Torok Molly , Choueiry Fouad , Kumar Rahul J. , Deems Anna , Miller Jenna L. , Hinton Alice , Geraghty Connor , Maranon Jose Angel , Kulp Samuel K. , Coss Christopher , Carson William E. , Conwell Darwin L. , Hart Phil A. , Cooperstone Jessica L. , Mace Thomas A. 
Published: December 2020

AbstractChronic pancreatitis (CP) is a fibro-inflammatory syndrome in individuals who develop persistent pathological responses to parenchymal injury or stress. Novel therapeutic or dietary interventions that could lessen inflammation in this disease could significantly improve quality of life in patients with CP. Complex dietary foods like soy and tomatoes are composed of active metabolites with anti-inflammatory effects. Data from our group reports that bioactive agents in soy and tomatoes can reduce pro-inflammatory cytokines and suppressive immune populations. Additionally, our team has developed a novel soy-tomato juice currently being studied in healthy individuals with no toxicities, and good compliance and bioavailability. Thus, we hypothesize that administration of a soy-tomato enriched diet can reduce inflammation and severity of CP. C57BL/6 mice were injected intraperitoneally with 50 μg/kg caeurlein (7 hourly injections, twice weekly) for 6 weeks to induce CP. After 4 weeks of caerulein injections, mice were administered a control or a soy-tomato enriched diet for 2 weeks. Disease severity was measured via immunohistochemical analysis of pancreata measuring loss of acini, fibrosis, inflammation, and necrosis. Serum lipase and amylase levels were analyzed at the end of the study. Inflammatory factors in the serum and pancreas, and immune populations in the spleen of mice were analyzed by cytokine multiplex detection, qRT-PCR, and flow cytometry respectively. Infra-red (IR) sensing of mice was used to monitor spontaneous activity and distress of mice. Mice fed a soy-tomato enriched diet had a significantly reduced level of inflammation and severity of CP (p = 0.032) compared to mice administered a control diet with restored serum lipase and amylase levels (p < 0.05). Mice with CP fed a soy-tomato diet had a reduction in inflammatory factors (TNF-α, IL-1β, IL-5) and suppressive immune populations (myeloid-derived suppressor cells; MDSC) compared to control diet fed mice (p < 0.05). Infra-red sensing to monitor spontaneous activity of mice showed that soy-tomato enriched diet improved total activity and overall health of mice with CP (p = 0.055) and CP mice on a control diet were determined to spend more time at rest (p = 0.053). These pre-clinical results indicate that a soy-tomato enriched diet may be a novel treatment approach to reduce inflammation and pain in patients with CP.
Nasopharyngeal carcinoma MHC region deep sequencing identifies HLA and novel non-HLA TRIM31 and TRIM39 loci
Authors: Ning Lvwen , Ko Josephine Mun-Yee , Yu Valen Zhuoyou , Ng Hoi Yan , Chan Candy King-Chi , Tao Lihua , Lam Shiu-Yeung , Leong Merrin Man-Long , Ngan Roger Kai-Cheong , Kwong Dora Lai-Wan , Lee Anne Wing-Mui , Ng Wai-Tong , Cheng Ashley , Tung Stewart , Lee Victor Ho-Fun , Lam Ka-On , Kwan Chung-Kong , Li Wing-Sum , Yau Stephen , Bei Jin-Xin , Lung Maria Li 
Published: December 2020

AbstractDespite pronounced associations of major histocompatibility complex (MHC) regions with nasopharyngeal carcinoma (NPC), causal variants underlying NPC pathogenesis remain elusive. Our large-scale comprehensive MHC region deep sequencing study of 5689 Hong Kong Chinese identifies eight independent NPC-associated signals and provides mechanistic insight for disrupted transcription factor binding, altering target gene transcription. Two novel protective variants, rs2517664 (Trs2517664 = 4.6%,P = 6.38 × 10−21) and rs117495548 (Grs117495548 = 3.0%,P = 4.53 × 10−13), map nearTRIM31andTRIM39/TRIM39-RPP21; multiple independent protective signals map nearHLA-Bincluding a previously unreported variant, rs2523589 (P = 1.77 × 10−36). The rareHLA-B*07:05allele (OR < 0.015,P = 5.83 × 10−21) is absent in NPC, but present in controls. The most prevalent haplotype lacks seven independent protective alleles (OR = 1.56) and the one with additional Asian-specific susceptibility rs9391681 allele (OR = 2.66) significantly increased NPC risk. Importantly, this study provides new evidence implicating two non-human leukocyte antigen (HLA) genes, E3 ubiquitin ligases,TRIM31andTRIM39, impacting innate immune responses, with NPC risk reduction, independent of classical HLA class I/II alleles.
Autoregulation of yeast ribosomal proteins discovered by efficient search for feedback regulation
Authors: Roy Basab , Granas David , Bragg Fredrick , Cher Jonathan A. Y. , White Michael A. , Stormo Gary D. 
Published: December 2020

AbstractPost-transcriptional autoregulation of gene expression is common in bacteria but many fewer examples are known in eukaryotes. We used the yeast collection of genes fused to GFP as a rapid screen for examples of feedback regulation in ribosomal proteins by overexpressing a non-regulatable version of a gene and observing the effects on the expression of the GFP-fused version. We tested 95 ribosomal protein genes and found a wide continuum of effects, with 30% showing at least a 3-fold reduction in expression. Two genes, RPS22B and RPL1B, showed over a 10-fold repression. In both cases the cis-regulatory segment resides in the 5’ UTR of the gene as shown by placing that segment of the mRNA upstream of GFP alone and demonstrating it is sufficient to cause repression of GFP when the protein is over-expressed. Further analyses showed that the intron in the 5’ UTR of RPS22B is required for regulation, presumably because the protein inhibits splicing that is necessary for translation. The 5’ UTR of RPL1B contains a sequence and structure motif that is conserved in the binding sites of Rpl1 orthologs from bacteria to mammals, and mutations within the motif eliminate repression.
Chance played a role in determining whether Earth stayed habitable
Authors: Tyrrell Toby 
Published: December 2020

AbstractEarth’s climate has remained continuously habitable throughout 3 or 4 billion years. This presents a puzzle (the ‘habitability problem’) because loss of habitability appears to have been more likely. Solar luminosity has increased by 30% over this time, which would, if not counteracted, have caused sterility. Furthermore, Earth’s climate is precariously balanced, potentially able to deteriorate to deep-frozen conditions within as little as 1 million years. Here I present results from a novel simulation in which thousands of planets were assigned randomly generated climate feedbacks. Each planetary set-up was tested to see if it remained habitable over a period of 3 billion years. The conventional view attributes Earth’s extended habitability solely to stabilising mechanisms. The simulation results shown here reveal instead that chance also plays a role in habitability outcomes. Earth’s long-lasting habitability was therefore most likely a contingent rather than an inevitable outcome.
Human-derived NLS enhance the gene transfer efficiency of chitosan
Authors: Bitoque Diogo B. , Morais Joana , Oliveira Ana V. , Sequeira Raquel L. , Calado Sofia M. , Fortunato Tiago M. , Simão Sónia , Rosa da Costa Ana M. , Silva Gabriela A. 
Published: December 2020

Abstract
Nuclear import is considered as one of the major limitations for non-viral gene delivery systems and the incorporation of nuclear localization signals (NLS) that mediate nuclear intake can be used as a strategy to enhance internalization of exogenous DNA.
In this work, human-derived endogenous NLS peptides based on insulin growth factor binding proteins (IGFBP), namely IGFBP-3 and IGFBP-5, were tested for their ability to improve nuclear translocation of genetic material by non-viral vectors. Several strategies were tested to determine their effect on chitosan mediated transfection efficiency: co-administration with polyplexes, co-complexation at the time of polyplex formation, and covalent ligation to chitosan. Our results show that co-complexation and covalent ligation of the NLS peptide derived from IGFBP-3 to chitosan polyplexes yields a 2-fold increase in transfection efficiency, which was not observed for NLS peptide derived from IGFBP-5.
These results indicate that the integration of IGFBP-NLS-3 peptides into polyplexes has potential as a strategy to enhance the efficiency of non-viral vectors.
Effects of interpersonal familiarity on the auditory distance perception of level-equalized reverberant speech
Authors: Demirkaplan Özgen , Hacıhabiboğlu Hüseyin 
Published: December 2020

Familiarity with sound sources is known to have a modulatory effect on auditory distance perception. However, the level of familiarity that can affect distance perception is not clearly understood. A subjective experiment that aims to investigate the effects of interpersonal familiarity on auditory distance perception with level-equalized stimuli is reported. The experiment involves a binaural listening task where different source distances between 0.5 and 16 m were emulated by convolving dry speech signals with measured binaural room impulse responses. The experimental paradigm involved level-equalized stimuli comprising speech signals recorded from different-gender couples who have self-reported to have known each other for more than a year with daily interaction. Each subject judged the distances of a total of 15 different speech stimuli from their partner as well as spectrally most similar and most dissimilar strangers, for six different emulated distances. The main finding is that a similar but unfamiliar speaker is localized to be further away than a familiar speaker. Another finding is that the semantic properties of speech can potentially have a modulating effect on auditory distance judgements.
Molekulare und funktionale Abklärung hereditärer Schwerhörigkeiten am Beispiel des SLC26A4-Gens
Authors: Roesch Sebastian , Bernardinelli Emanuele , Wortmann Saskia , Mayr Johannes A. , Bader Ingrid , Schweighofer-Zwink Gregor , Rasp Gerd , Dossena Silvia 
Published: December 2020

ZusammenfassungDie in den vergangenen Jahren zunehmende Verfügbarkeit molekularer Untersuchungstechniken führt zu einer steigenden Anzahl diagnostizierter genetischer Varianten im Genom untersuchter Patienten. Am Beispiel der hereditären Innenohrschwerhörigkeit wird bei Betrachtung der Vielzahl publizierter Berichte über die mögliche Beteiligung einzelner Gene an der Pathogenese deutlich, welche Herausforderungen die Zunahme molekularer Informationen für die Interpretation mit sich bringt.In dieser Übersichtsarbeit werden anhand des Beispiels des SLC26A4-Gens und den damit verbundenen Formen einer hereditären Innenohrschwerhörigkeit die derzeit vorhandenen Möglichkeiten einer systematischen Abklärung und Interpretation dargestellt. Neben einer Beschreibung der physiologischen Funktion des resultierenden Pendrin-Proteins werden molekulare Untersuchungsmöglichkeiten zur Beurteilung der Funktion im Fall von nachgewiesenen Genvarianten erläutert. Die im Rahmen eines primär wissenschaftlichen Ansatzes resultierenden Ergebnisse auf molekularer Ebene dienen im klinischen Alltag der Interpretation hinsichtlich des kausalen Zusammenhangs zwischen einer nachgewiesenen Genvariante (Genotyp) und der Innenohrschwerhörigkeit (Phänotyp). Schließlich wird auf die mögliche Notwendigkeit einer weiteren interdisziplinären Abklärung, z. B. mithilfe einer Perchlorat-Testung der Schilddrüse, sowie therapeutische Möglichkeiten eingegangen.
Unusual, Radical Treatment of Type A Aortic Dissection: A Case Report
Authors: Benvenga Rossella M. , Bellino Michele , Mastrogiovanni Generoso , Triggiani Donato , Citro Rodolfo , Masiello Paolo , Galasso Gennaro , Piscione Federico , Iesu Severino 
Published: December 2020

AbstractType A aortic dissection, according to Stanford classification, is a surgical emergency with high morbidity and carries 56% of in-hospital mortality when surgical intervention is not performed. The surgical mortality at 30 days is 10 to 20%. The therapeutic goals are to replace the diseased ascending aorta and to treat or to monitor the distal aortic patent false lumen. When the dissection involves the aortic root and the architecture of aortic valve is normal, the surgical techniques used could be multiple: reinforce the aortic root and spare the native aortic valve or replace the aortic valve and the aortic root. The Florida sleeve technique has been developed to treat the aortic aneurysm, sparing the aortic valve in patients with connective tissue disease. Some case reports have described the use of this technique to treat an acute aortic dissection. In the following case, we present a single stage repair of the ascending aorta, aortic arch, and proximal intrathoracic aorta in a patient with Type A aortic dissection through the contemporaneous use of two techniques: Florida sleeve and Vascutek “Thoraflex” hybrid prosthesis. The use of these two techniques allows the repair/replacement of the proximal intrathoracic aorta, the sparing of the native aortic valve, the employment of a hybrid prosthesis to replace the supraortic vessels, and the creation of a descending aortic landing zone for later, distal intervention.
Risk of Acute Kidney Injury after Intravenous Contrast Media Administration in Patients with Suspected Pulmonary Embolism: A Propensity-Matched Study
Authors: Elias Adi , Aronson Doron 
Published: December 2020

Abstract
Background Although computed tomography pulmonary angiography (CTPA) is the preferred diagnostic procedure in patients with suspected pulmonary embolism (PE), some patients undergo ventilation/perfusion (V/Q) lung scan due to concern of contrast-associated acute kidney injury (AKI).
Methods The study used a cohort of 4,565 patients with suspected PE. Patients who received contrast during CTPA were compared with propensity score-matched unexposed control patients who underwent V/Q lung scanning. AKI was defined as ≥50% increase in serum creatinine during the first 72 hours after either CTPA or V/Q lung scan.
Results Classification and regression tree analysis demonstrated that baseline creatinine was the strongest determinant of the decision to use CTPA. Propensity-score matching yielded 969 patient pairs. There were 44 AKI events (4.5%) in patients exposed to contrast media (CM) and 33 events (3.4%) in patients not exposed to CM (risk difference: 1.1%, 95% confidence interval [CI]: −0.6 to 2.9%; odds ratio [OR]: 1.39, 95% CI: 0.86–2.26; p = 0.18). Using different definitions for AKI and extending the time window for AKI diagnosis gave similar results. In a sensitivity analysis with the inverse probability weighting method, the OR for AKI in the CTPA versus V/Q scan was 1.14 (95% CI: 0.72–1.78; p = 0.58).
Conclusion Intravenous contrast material administration was not associated with an increased risk of AKI in patients with suspected PE. Given the diagnostic superiority of CTPA, these results are reassuring with regard to the use of CTPE in patients with suspected PE perceived to be at risk for AKI.
Hydrogen–Deuterium Exchange Mass Spectrometry Identifies Activated Factor IX-Induced molecular Changes in Activated Factor VIII
Authors: van Galen Josse , Freato Nadia , Przeradzka Małgorzata A. , Ebberink Eduard H.T.M. , Boon-Spijker Mariëtte , van der Zwaan Carmen , van den Biggelaar Maartje , Meijer Alexander B. 
Published: December 2020

AbstractHydrogen–deuterium exchange mass spectrometry (HDX-MS) was employed to gain insight into the changes in factor VIII (FVIII) that occur upon its activation and assembly with activated factor IX (FIXa) on phospholipid membranes. HDX-MS analysis of thrombin-activated FVIII (FVIIIa) revealed a marked increase in deuterium incorporation of amino acid residues along the A1–A2 and A2–A3 interface. Rapid dissociation of the A2 domain from FVIIIa can explain this observation. In the presence of FIXa, enhanced deuterium incorporation at the interface of FVIIIa was similar to that of FVIII. This is compatible with the previous finding that FIXa contributes to A2 domain retention in FVIIIa. A2 domain region Leu631-Tyr637, which is not part of the interface between the A domains, also showed a marked increase in deuterium incorporation in FVIIIa compared with FVIII. Deuterium uptake of this region was decreased in the presence of FIXa beyond that observed in FVIII. This implies that FIXa alters the conformation or directly interacts with this region in FVIIIa. Replacement of Val634 in FVIII by alanine using site-directed mutagenesis almost completely impaired the ability of the activated cofactor to enhance the activity of FIXa. Surface plasmon resonance analysis revealed that the rates of A2 domain dissociation from FVIIIa and FVIIIa-Val634Ala were indistinguishable. HDX-MS analysis showed, however, that FIXa was unable to retain the A2 domain in FVIIIa-Val634Ala. The combined results of this study suggest that the local structure of Leu631-Tyr637 is altered by FIXa and that this region contributes to the cofactor function of FVIII.
Arthroscopic Removal of an Intra-Articular Migrating Plant Awn Associated with Septic Shoulder Joint Arthritis in a Dog
Authors: Camarasa Juan J. , Travetti Olga , Vallefuoco Rosario 
Published: December 2020

Abstract
Objective The aim of this study was to describe the clinical presentation, advanced imaging findings and outcomes of arthroscopic treatment of a bacterial septic shoulder arthritis caused by the penetration of a migrating plant awn in a dog.
Case Report A 10-month-old, 11 kg, sexually intact male Cocker Spaniel was diagnosed with septic arthritis of the right scapulohumeral joint. Computed tomography revealed marked articular effusion and thickening of the capsule of the right shoulder joint. Ultrasound of the shoulder identified a bilinear hyperechoic structure located within the joint consistent with a plant (grass) awn. Arthroscopy was used for foreign body removal. Antibiotic treatment was prescribed according to bacteriologic sensitivity tests. Treatment was successful: the dog completely recovered and under high levels of activity showed neither clinical signs nor recurrence of lameness on follow-up at 6 and 24 weeks after surgery.
Conclusion Migrating plant awn can be the cause of septic arthritis in a dog. The combination of advanced imaging techniques and arthroscopy allowed accurate diagnosis, reduced the risk of surgical complications and shortened recovery time in this patient. Considering the benefits of minimally invasive techniques, arthroscopy represents an excellent option for the treatment of dogs with similar intra-articular foreign bodies.
Ruptured Aneurysm of the Sinus of Valsalva Causing Pulmonary Embolism: A Rare Association
Authors: Barroso Francisco V. C. , Takakura Isabela T. , Reis Ricardo C. , Valente Acrisio S. , Lima Neiberg A. , Gomes Camila R. F. , Justo Neto Júnior José , Sobrinho Carlos R. M. Rodrigues 
Published: December 2020

AbstractAneurysms of the sinus of Valsalva are rare. Unruptured sinus of Valsalva aneurysm is usually asymptomatic and rarely presents as right ventricular outflow obstruction, myocardial infarction as a result of coronary artery compression, conduction disturbances, or endocarditis. They have only been reported as the presumed source of embolism in six cases. We report a patient with right sinus of Valsalva rupture to the right atrium and embolization of aneurysm contents to the pulmonary vasculature.
Fandom and identity construction: an analysis of Thai fans’ engagement with Twitter
Authors: Smutradontri Pitchapa , Gadavanij Savitri 
Published: December 2020

AbstractIn our digital era, fandom has become a social and cultural phenomenon, notably in Thailand. Fans are dedicated, and creating fan text (i.e., text production made by fans about their object of fandom) is one way of showing dedication and passion to the fan base. This article explores how Thai fans engage with fan text on popular social media platforms such as Twitter, and how fandom relates to identity construction among Thai fans who are online media users. The results from a selected sample comprising 100 fan tweets from four different sources, suggests five types of fan tweets, including: hypothetical interpretation, fan art, narrative concerning an anecdote regarding the source text, expression of personal opinions and feelings, and fan parody. Moreover, this article discusses fans’ shared lexicon called ‘fan talk,’ and how fans position themselves as relatives and friends of the source texts. This article further discusses the humorous nature and the transcultural elements found in fan tweets, especially the ‘Thai-ifize’ method that fans use in creating fan tweets.
A relevance-theoretic account of translating jokes with sexual innuendos inModern Familyinto Spanish
Authors: Díaz-Pérez Francisco Javier 
Published: December 2020

AbstractThe main purpose of this paper is to analyse jokes containing sexual innuendos in ambiguous utterances from the first two seasons ofModern Familyand their translation into Spanish using relevance theory. More often than not, the ambiguity and sexual innuendos are also reflected in the Spanish versions analysed. Hence, in all those cases, in relevance-theoretical terms, the cognitive effects intended in the source text (ST), including humorous ones, will also be accessible to target text (TT) viewers. It, therefore, follows that the pragmatic scenario is preserved in the TT, sometimes at the expense of a sacrifice in the semantic scenario. In audio-visual texts, ambiguity may also impact the visual channel. Although in some cases the visual component may render the translator’s task difficult, in others it may act as an aid to both the translator and TT viewer, contributing to the yielding of humorous effects.
Admonishing
Authors: Kádár Dániel Z. , House Juliane , Liu Fengguang , Song Yulong 
Published: December 2020

Abstract
This paper examines the pragmatic properties of what we define as a ritual act of ‘admonishing’. We argue that admonishing
represents a historically embedded realisation type of the speech act Suggest. We explore admonishing in ancient Chinese political and
governance texts dated before the 2nd century BC. The results of our analysis show that admonishing often not only affords but even triggers
paradoxical pragmatic behaviour. This paradox stems from the fact that, in many historical linguacultures like the ancient Chinese,
admonishing was directed at a recipient, most typically a ruler, who was more highly ranked than the admonisher himself. While this context
normally precluded threatening the recipient’s face, admonishing was realised in a ritual frame in which such a face threat was
deindividuated. In pragmatics, a research gap exists in the study of the historical act of admonishing, and so the current study fills a
knowledge gap.