bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGARUH POLYETHYLENE GLYCOL (PEG) TERHADAP KADAR KUERSETIN KULTUR KALUS Chrysanthemum morifolium Ramat PADA KONDISI PENCAHAYAAN BERBEDA
Authors: Setiawati Tia , Zazuli Syifa Fauzia , Annisa Annisa , Nurzaman Mohamad , Irawan Budi 
Published: April 2020

AbstrakKrisan (Chrysanthemum morifolium Ramat) mengandung senyawa kuersetin dengan efek farmakologi yang sangat luas. Penambahan polyethilene glycol (PEG) dapat dimanfaatkan untuk meningkatkan produksi metabolit sekunder secara in vitro. Penelitian ini bertujuan untuk mengetahui pengaruh PEG terhadap pertumbuhan kalus krisan dan kadar kuersetin 3-O-rhamnosida pada pencahayaan yang berbeda. Penelitian menggunakan Rancangan Acak Lengkap (RAL). Eksplan kalus berumur 45 hari setelah tanam (HST) disubkultur pada media MS + 4 ppm 2,4-D dengan penambahan PEG dalam lima taraf konsentrasi yaitu 0, 10, 20, 30, dan 40 ppm. Kultur diinkubasi pada kondisi gelap dan terang. Parameter yang diamati adalah warna, tekstur, ukuran, berat basah, dan berat kering kalus serta kadar kuersetin 3-O-rhamnosida. Data kuantitatif dianalisis menggunakan Analisis Varians dan Uji Jarak Berganda Duncan (α 5%), sedangkan data kualitatif dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa kalus pada kondisi terang berwarna cokelat dan cokelat kehijauan, sedangkan pada kondisi gelap berwarna putih kecokelatan. Perlakuan 10 ppm PEG menghasilkan berat basah dan berat kering kalus tertinggi baik pada kondisi terang maupun gelap berturut-turut 1,97 g dan 2,92 g; 0,94 g dan 1,09 g. Kadar kuersetin 3-O-rhamnosida tertinggi pada kondisi gelap dan terang terdapat pada perlakuan 10 ppm PEG berturut-turut 1,72 µg/g berat kering (BK) dan 2,59 µg/g BK.Abstract Chrysanthemum morifolium Ramat. contains quercetin with very broad pharmacological effects. The addition of PEG can be used to increase the production of secondary metabolites using in vitro method. This study aimed to determine the effect of PEG on the growth of Chrysanthemum callus and quercetin 3-O-rhamnoside content in different lighting conditions. The experimental design used was a Completely Randomized Design. After 45 days, callus were subcultured on MS medium + 4 ppm 2,4-D which PEG was added in five concentration levels (0, 10, 20, 30, and 40 ppm). Culture was incubated in dark and light conditions. Parameters observed were color, texture, size, wet weight and dry weight of callus, also quercetin 3-O-rhamnoside levels. Quantitative data was analyzed using Analysis of Variance and Duncan's Multiple Distance (α 5%). Qualitative data was analyzed descriptively. The results showed that in light condition, the callus has brown and greenish brown color, whereas in dark, it has brownish white color. The 10 ppm PEG treatment produced the highest fresh weight and dry weight in both light and dark conditions of 1.97 g and 2.92 g, 0.94 g and 1.09 g, respectively.The highest quercetin 3-O-rhamnoside content in dark and light conditions were founded in 10 ppm PEG treatment of 1.72 µg/g dry weight (DW) and 2.59 µg/g DW.
Method for in vitro production of cartilage microtissues from scaffold-free spheroids composed of human adipose-derived stem cells
Authors: Tu Vy Thi-Kieu , Le Ha Thi-Ngan , To Xuan Hoang-Viet , Nguyen Phuc Dang-Ngoc , Huynh Phat Duc , Le Thuan Minh , Vu Ngoc Bich 
Published: April 2020

Introduction: Cartilage damage is one of the injuries that is difficult for the human body to self-repair due to the avascular and completely mature tissue with only few stem or progenitor cells present. Recently, some studies showed that engineered cartilage tissues could be used to treat or improve this injury. This study aimed to produce the cartilage microtissues from the differentiation of scaffold-free spheroids composed of human adipose-derived stem cells.
Methods: Human adipose-derived stem cells (ADSCs) were isolated and expanded following the previously published study. They were then cultured in the non-adherent condition to produce spheroids. The spheroids of the ADSCs were collected and induced into cartilage microtissues in the inducible medium for 21 days. The cartilage microtissue was characterized by some cartilage phenotype markers, including the accumulation of extracellular matrix proteins (aggrecan, glycosaminoglycan, and type II collagen), and the expression of certain genes specific to chondrocytes (Sox9, Col2, Col1, and Acan).
Results: The results showed that the expression of chondrocyte-specific genes gradually increased during the 21 days of culture for differentiation. On day 21, the microtissues expressed aggrecan, glycosaminoglycan, and type II collagen proteins.
Conclusion: This study demonstrated that cartilage microtissues could easily be produced from scaffold-free spheroids from ADSCs. Thus, cartilage microtissues can be produced in this manner for in vivo transplantation to promote cartilage regeneration, or to produce cartilage tissues for in vitro studies.
Method for Evaluating Security of Cloud IT-Components based on Estandards Criteria
Authors: Livshitz Ilya 
Published: April 2020

The analysis of well-known methods for ensuring IT-security is presented, methods for evaluating security of IT-components and Cloud services in general are considered.
An attempt to analyze cloud services not from a commercial position of a popular marketing product, but from a position of system analysis is made. The previously introduced procedure for IT-components evaluation is not stable, since the end user has not a 100% guarantee of access to all IT-components, and even more so to the remote and uncontrolled Cloud service. A number of reviews point at increased efforts to create a secure network architecture and ability to continuously monitor deviations from established business goals. In contrast to the Zero Trust and Zero Trust eXtended models, according to which additional security functions are superimposed on existing IT-components, it is proposed to consider the set of IT-components as a new entity – an Information Processing System. This will allow to move to formal processes for assessing the degree of compliance with the criteria of standards for both existing and prospective IT-components while ensuring security of Cloud services.
A new method for evaluation which is based on the previously developed hybrid methodology using formal procedures based on two systems of criteria - assessment of the degree of compliance of Management systems (based on ISO/IEC 27001 series) and assessment of functional safety requirements (based on IEC 61508 series and ISO/IEC 15408 series) is proposed. This method provides reproducible and objective assessments of security risks of Cloud-based IT‑components that can be presented to an independent group of evaluators for verification. The results obtained can be applied in the independent assessment, including critical information infrastructure objects.
Orszak wdzięcznych marionetek, czyli maszyna jako urzekający potwór
Authors: Wieczorek Krzysztof 
Published: April 2020

Autor artykułu stawia pytania o granice relacji człowiek – maszyna. Zwraca uwagę na to, że o ile w czasach dominacji idei mechanicyzmu panowało przekonanie o istnieniu ostrej, nienaruszalnej granicy między mechanizmem (do której to kategorii zaliczano również organizmy biologiczne, włącznie z ludzkim ciałem) a umysłem, będącym atrybutem istot obdarzonych duszą, o tyle współcześnie ta granica ulega zatarciu. Obserwujemy z jednej strony proces „umaszynowienia człowieka”, związany z rozmaitymi praktykami tworzącymi nurt transhumanizmu, a z drugiej strony – symetryczny (choć nie do końca) proces „ucieleśnienia umysłu”, który można też obrazowo określić jako „uczłowieczenie maszyny”. Intensywnie się rozwijający nurt badań i eksperymentów zmierzający do powstawania coraz doskonalszych form sztucznej inteligencji musi prędzej czy później natrafić na kolejną granicę: autonomii i podmiotowości obiektów wyposażonych w inteligencję. Nie pytając o techniczną stronę owego procesu, autor zastanawia się nad jego odzwierciedleniem i reperkusjami w filozofii, a także w świadomości społecznej. Podejmuje refleksję nad kryteriami, które pozwolą dostrzec, kiedy ten moment nastąpi, oraz nad przyszłością stosunków między człowiekiem a światem maszyn w sytuacji, gdy maszyna stanie się równorzędnym partnerem człowieka w ­życiu intelektualnym.
Les Mairuuwas - Masters of Water
Authors: Waage Trond 
Published: April 2020

‘LES MAIRUUWAS’ (The Masters of Water) is an ethnographic film about young male migrants and their dreams of succeeding in the city.
The filmmaker has followed a milieu of water transporters in urban Cameroon over many years. The four men portrayed, are among the thousands uneducated poor, that annually migrates from the Central African Republic to Cameroon searching a better life. The film describes their daily struggles to make a living and create meaning in harsh and highly vibrant urban surroundings.
We meet Coco the youngest of them, as he prepares to get his own room after years on the street. We follow Uncle as he strives to earn enough money to take care of his son. Abel expresses the felt stigma and his deeply desired wish for another way of life, after more than 15 years as a water transporter. Their belonging as a group and their possibilities to get work in the neighborhood is dramatically challenged when Bachirou is arrested.
PENGEMBANGAN MODEL WISATA EDUKASI DI MUSEUM PENDIDIKAN NASIONAL
Authors: Juwita Tita , Novianti Evi , Tahir Rusdin , Nugraha Awaludin 
Published: April 2020

ABSTRAKMuseum Pendidikan Nasional merupakan museum yang mempunyai konsep modern dan kini telah banyak di kenal oleh para pengunjung khususnya di kalangan mahawasiswa dan peserta didik karena dari jumlah kunjungan yang datang mengunjungi Museum Pendidikan Nasional merupakan Mahasiswa dan Peserta Didik yang merupakan salah satu tuntutan dalam kurikulum. Dalam upaya pengembangan wisata edukasi di Museum Pendidikan Nasional masih terdapat beberapa kendala yaitu prosedur alur kepemanduan yang belum efektif karena sistem kepemanduan yang kurang sesuai dengan kebijakan pendidikan di museum, maka perlu adanya kebijakan-kebijakan pendidikan dan menerapkan sistem wisata edukasi dengan baik agar pelaksanaan program wisata edukasi di Museum Pendidikan Nasional berjalan dengan efektif agar dapat meningkatkan jumlah kunjungan. Penelitian ini bertujuan untuk mengetahui pengembangan wisata edukasi di Museum Pendidikan Nasional. Penelitian ini di lakukan untuk memberikan edukasi pada wisatawan khususnya pengunjung museum agar mengetahui seberapa besar penting nya wisata edukasi di Museum. Metode penelitian yang digunakan adalah metode kualitatif. Data yang diambil adalah data primer dan data skunder, dimana data primer diperoleh dari kuesioner dan wawancara kepada narasumber yang telah ditentukan yaitu wawancara dengan staf Museum Pendidikan Nasional mengenai prosedur wisata edukasi yang di terapkan oleh museum dan data skunder yang di peroleh dari Museum Pendidikan Nasional yaitu berupa jumlah kunjungan, sejarah museum, struktur organisasi dan visi-misi museum. Kata Kunci: Museum Pendidikan, Pengembangan, Wisata Edukasi EDUCATION TOURISM DEVELOPMENT MODELS IN THE NATIONAL EDUCATION MUSEUM ABSTRACTThe National Education Museum is a museum that has a modern concept and is now widely known by visitors, especially in students and students because of the number of visits that come. the implementation system is not in accordance with the educational policies in the museum, it is necessary to have educational policies and tourism education system needs to be done well in order to run an educational tourism program in the National Education Museum running effectively in order to increase the number of visits. This research aims to study the development of tourism education in the National Education Museum. This research was conducted to provide education to special tourists who visit museums to be able to know the importance of tourism education in the Museum. The research method used is a qualitative method. The data taken are primary data and secondary data, while primary data obtained from questionnaires and interviews with predetermined sources, namely interviews with staff of the National Education Museum regarding tourist education procedures applied by the museum and secondary data obtained from the National Education Museum consists of number of visits, museum history, organizational structure and vision and mission of the museum. Keywords: Development, Educational Museum, Educational Tourism
RESPON PEMBACA TERHADAP APLIKASI TRAVELOKA: FENOMENA FITUR ‘TRAVELOKA XPERIENCE’
Authors: Rachmawati Iin 
Published: April 2020

ABSTRAK Akhir-akhir ini bisa dilihat bahwa ada banyak sekali cara-cara yang bisa dilakukan ketika seseorang hendak bepergian keliling dunia atau ketika hendak mencari hiburan.Internet menyediakan segala hal yang kita butuhkan hanya dengan beberapa kali klik. Kali ini akan dibahas mengenai fenomena baru yang biasa disebut ‘Traveloka Xperience’. Dengan fitur ini, para pengguna bisa mencari apapun terkait media hiburan yang mereka inginkan dalam satu aplikasi. Penelitian ini akan mencoba untuk menganalisis tentang respon pembaca terhadap ‘Traveloka Xperience’ sebagai platform media hiburan khususnya bagi mereka yang tinggal di Surabaya. Teori respon pembaca oleh Jauss Iser digunakan dalam penelitian ini. Pendekatan fenomenologi akan digunakan untuk membantu menemukan pemahaman tentang respon pembaca terhadap fenomena budaya tersebut. Hasilnya menunjukkan bahwa respon pelanggan terhitung sangat baik dalam menginterpretasikan manfaat dari fitur Traveloka Xperience. Para pengguna tampak menemukan makna dan manfaat dari fitur ini berdasarkan pada pengalaman dan latar belakang mereka. Kata Kunci : Media Hiburan, Respon Pembaca, Traveloka Xperience ABSTRACTRecently, there are numerous different ways that can be done by people when it comes to travel around the world or even when they just want to entertain themselves. Internet has provided everything they need by just a few simple clicks. Today, there is a new feature called ‘Traveloka Xperience’ in which can be said to be the new phenomenon in searching for any entertainment media. This study will try to analyze about the reader response of ‘Traveloka Xperience’ as a media for entertaining people especially for those who live in Surabaya. The theory of reader response from Jauss Iser will be used to conduct this study. Phenomenological approach will be used as the research design in order to help to find an understanding on how the readers will respond to this cultural phenomenon. The result shows that the users’ responds are quite good in interpreting the benefits of Traveloka Xperience feature. Those users can make the maximum benefits of finding the meaning of this feature based on their own experiences and backgrounds.
ROTI GAMBANG, ACCULTURATION BREAD FROM BETAWI
Authors: Lestari Nurul Sukma 
Published: April 2020

ABSTRAKTujuan dari penelitian ini adalah untuk mengetahui sejarah dari Roti Gambang, keunikan dan cara melestarikan keberadaan roti ini. Pemilihan roti gambang disebabkan karena roti ini adalah makanan traditional khas Betawi. Metode yang digunakan adalah metode kualitatif dengan pendekatan studi literatur. Hasil yang diperoleh dari penelitian ini adalah Roti Gambang berasal dari era kolonial. Keunikan roti ini adalah bentuknya yang persegi yang mengadopsi bentuk salah satu alat musik dari Gambang Kromong, yaitu Gambang serta penggunaan kayu manis dan gula merah. Roti ini mencapai popularitasnya pada akhir 1980-an dan 1990-an. Roti Gambang sudah mulai dijual di toko makanan yang ada di jaringan bioskop terbesar di Indonesia dan sudah tersedia juga di salah satu gerai Starbuck Comunnity Store di Jakarta. Disarankan untuk menjaga keberadaan Roti Gambang karena roti tersebut memiliki nilai sejarah dan terpilih menjadi salah satu roti paling enak di dunia berdasarkan CNN. Kata Kunci : Betawi, Nilai Sejarah, Roti GambangABSTRACTThe purpose of this research is to find out the history of Roti Gambang, the uniqueness and how to preserve the existence of this bread. Roti Gambang has been chosen because it's a traditional bread from Betawi. A qualitative method with a literature study approach used in this research. The results from this research are the bread originating from the colonial era. The uniqueness is a square shape that adopts the shape of Gambang, the musical instruments from Gambang Kromong and the use of cinnamon and brown sugar. Reached the popularity in the late 1980s and 1990s. The Bread is available at food stores in the largest cinema chain in Indonesia at one of the Starbuck Community Store outlets in Jakarta. It's recommended to maintain the existence of the Roti Gambang because of historical value and it was chosen to be one of the most delicious bread in the world based on CNN. Keywords: Betawi, Historical Value, Roti Gambang
PENGARUH KUALITAS NIGHTLIFE ATTRACTION TERHADAP KEPUASAN WISATAWAN DI KAWASAN PRAWIROTAMAN
Authors: Kholifah Nur Asriatul , Setiawan Bakti , Sunaryo Bambang 
Published: April 2020

ABSTRAKNightlife attraction merupakan komponen produk wisata yang penting tetapi kurang mendapat perhatian. Tujuan dari penelitian ini adalah mengetahui bagaimana wisata malam memiliki pengaruh pada kepuasan wisatawan terhadap kawasan Prawirotaman. Penelitian ini merujuk pada teori kualitas produk wisata yang mencakup atraksi wisata, amenitas, dan aksesibilitas yang menggunakan metode kuantitatif didukung dengan data kualitatif. Pengambilan data dilakukan dengan kuesioner pada wisatawan dan wawancara kepada pihak pengelola kemudian dianalisis dengan analisa Servqual, CSI, dan IPA. Hasil penelitian ini menunjukkan bahwa kualitas nightlife di Kawasan Prawirotaman masih jauh di bawah tingkat harapan wisatawan. Hal ini mengindikasikan tingkat kepuasan wisnus maupun wisman terhadap kawasan wisata Prawirotaman masih rendah. Terdapat beberapa indikator yang mempengaruhi tingkat kepuasan wisnus dan wisman yang dapat menjadi perhatian bagi pengelola dan pemerintah dalam meningkatkan kualitas nightlife attraction. Kesimpulan dari penelitian ini yaitu rendahnya kualitas elemen produk 3A (atraksi, amenitas, dan aksesibilitas) mempengaruhi kepuasan wisatawan secara keseluruhan. Kata Kunci : Nightlife Attraction, Kepuasan Wisatawan, Kawasan Wisata INFLUENCE OF QUALITY OF NIGHT ATTRACTION PRODUCTS TO TOURISTS SATISFACTION IN PRAWIROTAMANABSTRACT ABSTRACTNightlife attraction is an important component of tourism product but less attention. The purpose of this research is to know how the night attraction has an influence on tourists satisfaction in Prawirotaman. This study refers to quality of tourism product’s theory that include attractions, amenity, and accessibility that uses quantitative methods supported by qualitative data. The data were collected by questionnaire on the tourists and interview to the manager then analyzed by Servqual, CSI, and IPA analysis. These results indicate the quality of nightlife attraction in Prawirotaman area is still far below the tourist’s expectations. This indicates the level of satisfaction from domestic and international tourists towards Prawirotaman are still low or very dissatisfied. There are several indicators that affect the level of domestic and international tourists satisfaction can be a concern for managers and governments in improving quality of night attraction. The conclusion is the low quality of 3A product element (attraction, amenities, and accessibility) influence the whole tourist satisfaction. Keywords: Nightlife Attraction, Tourist Satisfaction, Tourism Area
Analysis on the Method of PLC Remote Monitoring and Fault Diagnosis
Authors: Zhou Ang 
Published: April 2020

<p>In the industry 4.0 era and under the framework of China's "Smart Manufacturing 2025" plan, efficient modern manufacturing equipment plays an important role in production. In order to ensure the safe and stable operation of production equipment, it is necessary to troubleshoot the fault in the first time. This paper discusses the method of remote monitoring and fault diagnosis based on PLC.</p>
OPTIMASI DAN EFISIENSI TEKNIK ISOLASI RNA DAUN DAN AKAR KELAPA SAWIT (Elaeis guineensis Jacq)
Authors: Sari Dini Astika , Martiansyah Irfan , Mukmin Restu Prasetya , Hadi Sapto Nugroho , Syahputra Indra , Afandi Dadang , Putranto Riza Arief 
Published: April 2020

Tanaman kelapa sawit memiliki kandungan polisakarida dan polifenol yang tinggi. Kontaminasi polisakarida dan polifenol menyebabkan sulitnya proses isolasi RNA dari jaringan tanaman kelapa sawit. Penelitian ini dilakukan untuk mengoptimasi beberapa protokol isolasi RNA tanaman kelapa sawit yang efektif dan efisien. Penelitian dilaksanakan selama tiga bulan, pada bulan Oktober-Desember 2018 di Laboratorium Biokimia dan Biologi Molekuler, Pusat Penelitian Bioteknologi dan Bioindustri Indonesia (PPBBI), Jalan Taman Kencana No. 1 Bogor 16128. Penelitian dilakukan dengan melaksanakan teknik isolasi RNA menggunakan tiga protokol, yaitu modifikasi Cetyl Trimethyl Ammonium Bromide (CTAB), kit isolasi RNA RNeasy Plant Mini Kit (Qiagen), dan kit isolasi NucleoSpin RNAPlant (Macherey-Nagel). Sampel yang digunakan adalah daun dan akar tanaman kelapa sawit berumur kurang dari tiga bulan dengan bobot 0,1 gram dan 2,5 gram yang disesuaikan untuk tiap protokol. Variabel yang diamati adalah konsentrasi (ng/µl), kemurnian (rasio A260/A280 dan A260/A230), dan pita RNA pada elektroforesis gel agarosa. Hasil penelitian menunjukkan, RNA total hasil isolasi protokol NucleoSpin RNAPlant (Macherey-Nagel) memiliki kualitas paling tinggi. Konsentrasi RNA total daun dan akar kelapa sawit yang didapatkan melalui protokol NucleoSpin RNAPlant (Macherey-Nagel) sebesar 338 ng/µl dan 184,4 ng/µl dengan rasio A260/A280 RNA total daun dan akar kelapa sawit sebesar 2,13 dan 2,18 serta rasio A260/A230 sebesar 2,09 dan 2,20. Hasil elektroforesis gel agarosa menunjukkan integritas yang bagus dari RNA total hasil isolasi RNeasy Plant Mini Kit (Qiagen) dan NucleoSpin RNAPlant (Macherey-Nagel), namun terdapat kontaminasi dan smear pada RNA total hasil isolasi CTAB modifikasi 1 dan 2. Kata kunci: kelapa sawit, isolasi RNA, spektrofotometer, elektroforesis gel agarosa
Penggunaan Zeolit Sebagai Media Penyaring Pada Pengolahan Air LimbahDomestik
Authors: Yanto Yanto 
Published: April 2020

<p>Sand filter is frequentlyused to treat contaminated water. Channel filter system is a modification of sand filter where the channel is shaped thus the land requirement could be minimized, water is flowed horizontallyfrom intial tank to finaltank through sand filter. Employing the channel filter to treat domestic wastewateris capable of reducing E. Coli up to 98.14%, increasing DO up to 27% and decreasing BOD5 up to 27%. Unfortunately, the final content of E.Coli after treatment process completed is still unacceptable for drinking water. Several options are available to improve the performance of the channel filter system. One of the promising alternativesis modifying the filter medium. Zeolite is natural material that has been utilized to improvewater quality based on several parameters such as Fe, Mn, organic materials, CO and others. Application of zeolite to diminish E. Coli is a challenge. This paper will investigate the effectiveness of zeolite to lower E. Coli contained in domestic wastewater . Zeolite was added to sand filter where thefraction of zeolite is about 5%. Two kind of zeolite-sand combination was implemented that is arranged and mixed zeolite-sand. Wastewater containing E.Coli was then put in the initial tank. Through the hole createdin the tank, wastewater then flowed passing through the combined zeolite-sand filter and accumulated in the final tank. Both E. Coli from initial and final tank was measured to compute treatment efficiency. The result showsE. Coli decreases up to99.99%, BOD5and DO decrease more than 71% and 66% respectively. It can be concluded that 5% addition ofzeolite is able to improve treatment efficiency ofsand filter.</p>