bluuebunny/crossref_metadata_2025_split huggingface dataset:
Airborne Influenza A Virus Exposure in an Elementary School
Authors: Coleman Kristen K. , Sigler William V. 
Published: February 2020

AbstractInfluenza contributes significantly to childhood morbidity and mortality. Given the magnitude of the school-aged child population, a sizeable proportion of influenza virus transmission events are expected to occur within school settings. However, influenza virus activity in schools is not well-understood, likely due to our limited ability to accurately monitor for respiratory viruses without disrupting the school environment. In this study, we evaluated the use of a bioaerosol sampling method to noninvasively detect and quantify airborne influenza A virus (IAV) densities in a public elementary school. Air samples were collected from multiple locations in the school, two days per week, throughout an eight-week sampling period during influenza season. Real-time RT-PCR targeting the IAV M gene revealed detectable IAV on five occasions in densities ranging from 2.0 × 10−1to 1.9 × 104. No significant differences in IAV densities were related to student presence/absence. The majority of IAV-associated particles were ≤4 μm in diameter, and theoretical calculations indicate infectious thresholds after minutes of exposure. Our study represents the first identification and quantification of airborne influenza virus in an elementary school, and the results suggest that airborne IAV has the potential to circulate in schools during influenza season, in large enough doses known to cause infection.
Remediation of hexavalent chromium contaminated water through zero-valent iron nanoparticles and effects on tomato plant growth performance
Authors: Brasili Elisa , Bavasso Irene , Petruccelli Valerio , Vilardi Giorgio , Valletta Alessio , Dal Bosco Chiara , Gentili Alessandra , Pasqua Gabriella , Di Palma Luca 
Published: February 2020

AbstractContaminated water with hexavalent chromium Cr(VI) is a serious environmental problem. This study aimed to evaluate the Cr(VI) removal by zero valent iron nanoparticles (nZVI) reduction process and the impact of Cr(VI), nZVI and combined treatment with nZVI and Cr(VI) on tomato growth performance. To evaluate the Cr(VI) toxic effect on germination capability, seeds were exposed to increasing Cr(VI) concentrations up to 1000 mg L−1. The inhibition of seed germination and the decrease of hypocotyl and root length started from Cr(VI) 5 mg L−1. Under treatment with Cr(VI) + nZVI 5 mg L−1, seed germination, hypocotyl and root length resulted significantly higher compared to Cr(VI) 5 mg L−1 treatment. The impact of only nZVI was investigated on chlorophyll and carotenoid in leaves; iron levels in leaves, roots, fruits and soil; carotenoid, fat-soluble vitamin and nicotianamine in mature fruits. A significant increase of leaf chlorophyll and carotenoids was observed after nZVI 5 mg L−1 treatment compared to controls. No significant variations were observed in carotenoids, fat-soluble vitamins and nicotianamine levels after treatment with nZVI 5 mg L−1 in mature fruits. For their ability to reduce Cr(VI) and to stimulate tomato growth, nZVI might to be considered as alternative for remediation purposes.
STAT5-dependent regulation of CDC25A by miR-16 controls proliferation and differentiation in FLT3-ITD acute myeloid leukemia
Authors: Sueur Gabrielle , Boutet Alison , Gotanègre Mathilde , Mansat-De Mas Véronique , Besson Arnaud , Manenti Stéphane , Bertoli Sarah 
Published: February 2020

AbstractWe recently identified the CDC25A phosphatase as a key actor in proliferation and differentiation in acute myeloid leukemia expressing the FLT3-ITD mutation. In this paper we demonstrate that CDC25A level is controlled by a complex STAT5/miR-16 transcription and translation pathway working downstream of this receptor. First, we established by CHIP analysis that STAT5 is directly involved in FLT3-ITD-dependent CDC25A gene transcription. In addition, we determined that miR-16 expression is repressed by FLT3-ITD activity, and that STAT5 participates in this repression. In accordance with these results, miR-16 expression was significantly reduced in a panel of AML primary samples carrying the FLT3-ITD mutation when compared with FLT3wt cells. The expression of a miR-16 mimic reduced CDC25A protein and mRNA levels, and RNA interference-mediated down modulation of miR-16 restored CDC25A expression in response to FLT3-ITD inhibition. Finally, decreasing miR-16 expression partially restored the proliferation of cells treated with the FLT3 inhibitor AC220, while the expression of miR-16 mimic stopped this proliferation and induced monocytic differentiation of AML cells. In summary, we identified a FLT3-ITD/STAT5/miR-16/CDC25A axis essential for AML cell proliferation and differentiation.
Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s
Authors: Roustan Valentin , Hilscher Julia , Weidinger Marieluise , Reipert Siegfried , Shabrangy Azita , Gebert Claudia , Dietrich Bianca , Dermendjiev Georgi , Schnurer Madeleine , Roustan Pierre-Jean , Stoger Eva , Ibl Verena 
Published: February 2020

AbstractCereal endosperm is a short-lived tissue adapted for nutrient storage, containing specialized organelles, such as protein bodies (PBs) and protein storage vacuoles (PSVs), for the accumulation of storage proteins. During development, protein trafficking and storage require an extensive reorganization of the endomembrane system. Consequently, endomembrane-modifying proteins will influence the final grain quality and yield. However, little is known about the molecular mechanism underlying endomembrane system remodeling during barley grain development. By using label-free quantitative proteomics profiling, we quantified 1,822 proteins across developing barley grains. Based on proteome annotation and a homology search, 94 proteins associated with the endomembrane system were identified that exhibited significant changes in abundance during grain development. Clustering analysis allowed characterization of three different development phases; notably, integration of proteomics data with in situ subcellular microscopic analyses showed a high abundance of cytoskeleton proteins associated with acidified PBs at the early development stages. Moreover, endosomal sorting complex required for transport (ESCRT)-related proteins and their transcripts are most abundant at early and mid-development. Specifically, multivesicular bodies (MVBs), and the ESCRT-III HvSNF7 proteins are associated with PBs during barley endosperm development. Together our data identified promising targets to be genetically engineered to modulate seed storage protein accumulation that have a growing role in health and nutritional issues.
Storm Response of Fluvial Sedimentary Microplastics
Authors: Ockelford Annie , Cundy Andy , Ebdon James E. 
Published: February 2020

AbstractUp to 80% of the plastics in the oceans are believed to have been transferred from river networks. Microplastic contamination of river sediments has been found to be pervasive at the global scale and responsive to periods of flooding. However, the physical controls governing the storage, remobilization and pathways of transfer in fluvial sediments are unknown. This means it is not currently possible to determine the risks posed by microplastics retained within the world’s river systems. This problem will be further exacerbated in the future given projected changes to global flood risk and an increased likelihood of fluvial flooding. Using controlled flume experiments we show that the evolution of the sediment bed surface and the flood wave characteristics controls the transition from rivers being ‘sinks’ to ‘sources’ of microplastics under flood conditions. By linking bed surface evolution with microplastic transport characteristics we show that similarities exist between granular transport phenomena and the behavior, and hence predictability, of microplastic entrainment during floods. Our findings are significant as they suggest that microplastic release from sediment beds can be managed by altering the timing and magnitude of releases in flow managed systems. As such it may be possible to remediate or remove legacy microplastics in future.
Comments on: A functional polymorphism rs10830963 in melatonin receptor 1B associated with the risk of gestational diabetes mellitus
Authors: Rosta Klara , Harreiter Jürgen , Nádasdi Ákos , Németh László , Kautzky-Willer Alexandra , Somogyi Anikó , Firneisz Gábor 
Published: February 2020

Abstract
We have read with great interest the accepted manuscript of the meta-analysis performed by Huang, et al. titled “A functional polymorphism rs10830963 in melatonin receptor 1B associated with the risk of gestational diabetes mellitus” published online in the 2019 December 6 issue of Bioscience Reports (https://doi.org/10.1042/BSR20190744).
We do agree with the authors’ final conclusion that such a meta-analysis should eventually confirm that the MTNR1B rs10830963 G allele is significantly associated with increased risk of gestational diabetes mellitus (GDM) development in pregnant populations with Asian and European ancestry. However we have surprisingly found that our genetic association study (PLoS One (2017), https://doi.org/10.1371/journal.pone.0169781) was included in this meta-analysis, but with mistakenly calculated odds ratios (OR). Therefore we would suggest to use the correct OR values based on our original publication that were already indicating a high genetic effect size for the MTNR1B rs10830963 risk variant on GDM development.
Reliability and Validity of the Kannada Version of the Consensus Auditory-Perceptual Evaluation of Voice
Authors: Gunjawate Dhanshree R. , Ravi Rohit , Bhagavan Srividya 
Published: February 2020

Purpose
The purpose of this study was to evaluate the reliability and validity of the Kannada version of the Consensus Auditory-Perceptual Evaluation of Voice (CAPE-V).

Method
The Kannada version of CAPE-V comprises six phrases that are phonetically designed as per the CAPE-V requirements. Sixty-five (21 individuals with dysphonia and 44 asymptomatic) participants were enrolled for the instrument psychometric validation. The interrater and intrarater reliability as well as validity were assessed.

Results
High level of agreement was noted between the three raters across all the CAPE-V parameters, highest for pitch (intraclass correlation coefficient value = .98) and lowest for loudness (intraclass correlation coefficient value = .96). High intrarater reliability agreement (intraclass correlation coefficient value > .97) was also noted for all the parameters. Among the correlation for parameters that are comparable between CAPE-V and the Grade, Roughness, Breathiness, Asthenia, and Strain scale, the highest correlation was noted for overall severity. There was a significant difference noted between the study and control groups for all parameters except loudness. The discriminant function analysis and classification revealed that 98% were correctly identified.

Conclusion
The Kannada version of CAPE-V has been proven to be a psychometrically reliable and valid tool to use for auditory-perceptual evaluation of voice.
Fundusautofluoreszenz: Klinische Anwendung und diagnostische Bedeutung
Authors: Bindewald-Wittich Almut , Kellner Ulrich 
Published: February 2020

ZusammenfassungDie Bildgebung mittels Fundusautofluoreszenz (FAF) ist ein nichtinvasives Verfahren, das Fluorophore vor allem auf Ebene der Fotorezeptoren, des Subretinalraums und des retinalen Pigmentepithels sichtbar macht und zusätzliche, wichtige Informationen liefert. Sie hat entscheidend zum pathophysiologischen Verständnis vieler Erkrankungen beigetragen und wird in Kombination mit anderen bildgebenden Verfahren u. a. für Diagnostik und Verlaufsbeobachtung eingesetzt.
OCT-Diagnostik beim Glaukom: Tipps & Tricks
Authors: Mardin Christian 
Published: February 2020

ZusammenfassungDie Technik der optischen Kohärenztomografie scheint bei Glaukomen mittlerweile unverzichtbar für Dokumentation, Diagnosehilfestellung und Verlaufsbeobachtung. Die kritisch betrachteten Befunde sollten immer in Übereinstimmung mit dem klinischen Bild des Patienten gesehen werden. Der Artikel zeigt die Einsatzmöglichkeiten anhand von Fallbeispielen.
Soziale Unterstützung oder soziale Ansteckung? Wie sich Betroffene mit Essstörungen im stationären und Wohngruppensetting gegenseitig beeinflussen
Authors: Wunderer Eva , Moll Christine , Kaltenhauser Theresa 
Published: February 2020

Zusammenfassung
Hintergrund Zusammenzuleben mit anderen Betroffenen kann für Menschen mit Essstörungen soziale Unterstützung bieten. Doch es sind auch soziale Ansteckungsprozesse möglich, wenn Konkurrenz entsteht und sich die Betroffenen in ihrer Symptomatik gegenseitig zu überbieten versuchen.
Methode In 2 qualitativen Studien wurden insgesamt 19 weibliche Betroffene im stationären Kontext bzw. Wohngruppensetting zu ihren Erfahrungen befragt.
Ergebnisse Die inhaltsanalytische Auswertung der problemzentrierten, leitfadengestützten Interviews zeigt Chancen, aber auch Risiken, gerade in reinen Essstörungs-Gruppen.
Diskussion Es scheint wichtig, dass eine Etikettierung von Betroffenen („die Esssgestörten“) und eine Fokussierung auf die Symptomatik vermieden, die Individualität und der Genesungswunsch gestärkt sowie Ansteckungsprozesse reflektiert werden.
Horizon-Scanning im Gesundheitswesen – wo steht Deutschland?
Authors: Krabbe Laura , Buchberger Barbara 
Published: February 2020

Zusammenfassung
Hintergrund Entscheidungsträger im Gesundheitswesen handeln in einem Umfeld, in dem Erwartungen steigen, neue nutzenbringende Technologien so schnell wie möglich verfügbar zu machen, Patienten vor gefährlichen oder unwirksamen Technologien zu schützen und im Zuge dessen die Diffusion einer Technologie zu be- oder entschleunigen. Horizon-Scanning (HS) ist ein Prozess, um frühzeitig einflussreiche neue und aufstrebende Technologien zu identifizieren und über deren potenzielle Auswirkungen auf das Gesundheitssystem zu informieren.
Ziel der Arbeit Darstellung der Methodik von HS, weltweit bestehender Aktivitäten und des Status quo in Deutschland.
Methodik Eine systematische Literaturrecherche wurde im April 2015 durchgeführt und durch Handsuchen ergänzt. Zudem erfolgte eine qualitative Befragung von Experten im deutschen Gesundheitssystem.
Ergebnisse Es konnten 27 formale HS-Systeme (HSS) weltweit identifiziert werden, von denen 18 dem „International Information Network on New and Emerging Health Technologies“ angegliedert sind. Im Wesentlichen werden dieselben Prozessschritte angewendet, können aber aufgrund unterschiedlicher Strukturen und Interessen der Gesundheitssysteme methodische Unterschiede aufweisen. In Deutschland existiert kein offizielles HSS. Die Befragung der Experten zeigte, dass zum Teil Unkenntnis herrscht und das Verständnis von HS variiert, grundsätzlich aber ein Bedarf zu bestehen scheint.
Schlussfolgerung Der systematische HS-Prozess kann an die Gegebenheiten und Ziele eines Gesundheitssystems angepasst werden und findet international Anklang. In Deutschland könnte HS dazu beitragen, Entscheidungsträgern und Interessengruppen frühzeitig Informationen zu verschaffen und sie bei ihren Entscheidungen zu unterstützen.
Necrotizing Infection of the Aortic Arch: Reconstruction Utilizing Unusual Extra-anatomic Bypass Grafts to Reroute Cerebral Blood Flow
Authors: Hemli Jonathan M. , Patton Byron D. , Scheinerman S. Jacob , Brinster Derek R. 
Published: February 2020

AbstractDestructive infections of the aortic arch and great vessels are challenging to manage. We describe a novel technique for debranching the right cerebral and upper extremity arteries via composite extra-anatomic bypasses from the femoral artery, with subsequent homograft in-line reconstruction of the arch, in a patient with Staphylococcus sepsis and necrosis of the arch and great vessels.
Amelioration of Diabetic Nephropathy in Streptozotocin-Induced Diabetic Rats by Acacia catechu Leaves Extract
Authors: D’souza Prima , Holla Rajendra , Swamy Gangadhara 
Published: February 2020

Abstract
Objective The present study was performed to evaluate the ethanolic extract of leaves of Acacia catechu (A. catechu) for its effect on streptozotocin (STZ)-induced diabetes mellitus (DM) and its renal complications in male Wistar albino rats.
Materials and Methods Male Wistar albino rats were grouped into control (A), STZ-induced DM (B), STZ-induced DM rats with A. catechu orally of 75 mg/kg body weight (kbw) for 35 days (C), with each group having six rats (n = 6) weighing between 200 to 250 g each. Group A receives only water, orally; group B receives a single dose of STZ at 45 mg/kbw intraperitoneal administration (IP); group C receives STZ IP and oral A. catechu for 35 days. On the 36th day, animals were euthanized, the kidney tissues were analyzed for biochemical parameters, such as GOT (glutamic oxaloacetic transaminase), GPT (glutamic pyruvic transaminase), oxidative stress assessment parameters, and histopathological studies.
Results In group C rats, activities of the enzymes were nearer to group A when compared with group B. Histopathological findings were also suggesting that renal toxicity were observed at a lesser extent in group C.
Conclusion The ethanolic extract of A. catechu signified as nephroprotective effect. The present data could provide adequate confirmation of the efficacy of ethanolic extract of leaves of A. catechu for further experimental studies on a standardized formulation.
Reduction of an in vitro Intraradicular Multispecies Biofilm Using Two Rotary Instrumentation Sequences
Authors: Zavattini Angelo , Cowie Jonathan , Niazi Sadia , Giovarruscio Massimo , Sauro Salvatore , Foschi Federico 
Published: February 2020

Abstract
Objective The purpose of this research was to investigate the effect per se of two shaping and cleaning techniques on the reduction of an in vitro multispecies biofilm.
Materials and Methods A total of 39 freshly extracted monoradicular teeth for periodontal reason were decoronated. Roots were sectioned longitudinally. After autoclaving, a specific stressed biofilm was grown on the root halves that were subsequently reassembled in a silicone index. Two treatments (n = 9 each)—RaCe (Schottlander; Letchworth Garden City, United Kingdom) and ProTaper Gold ( PTG; Dentsply Maillefer, Baillagues, Switzerland)—were tested; three noninstrumented samples served as a control group and three were rinsed with saline. Posttreatment samples were taken at three different levels of the root. Colony-forming units were counted after incubations. Additionally, three treatments (n = 5 each)—RaCe, PTG, and saline only—were evaluated under a confocal laser scanning microscope (CLSM).
Statistical Analysis Statistical analysis was conducted using Tukey’s test and analysis of variance to evaluate the post-instrumentation bioburden.
Results Both instrumentations were able to reduce the biofilm; however, differences were not present between them (p > 0.05). CLSM showed biofilm killing and disruption through mechanical shaping alone.
Conclusions Intraradicular biofilm is reduced with mechanical shaping. There was no difference between RaCe and PTG systems in biofilm reduction despite differences in design, file sequence, and rotational speed.
Logophoricity and shifts of perspective
Authors: Nikitina Tatiana 
Published: February 2020

AbstractThis study presents a typology of existing approaches to logophoricity and discusses problems the different approaches face. It addresses, in particular, perspective-based accounts describing constructions with logophoric pronouns in terms of their intermediate position on the direct-indirect continuum (Evans 2013), and lexical accounts incorporating the idea of coreference with the reported speaker into the pronoun’s meaning, either through role-to-value mapping mechanisms (Nikitina 2012a,b), or through feature specification (Schlenker 2003a,b). The perspective-based approach is shown to be unsatisfactory when it comes to treating language-specific data in precise and cross-linguistically comparable terms. It fails to account, for example, for cross-linguistic differences in the behavior of logophoric pronouns, for their optionality, and for their close diachronic relationship to third person elements. Lexical accounts are better equipped to handle a variety of outstanding issues, but they, too, need to be revised to accommodate a variety of discourse phenomena associated with logophoricity, including alternation with first person pronouns. The proposed solution follows the lines of lexical approaches but aims at enriching the pronouns’ lexical representation with notions pertaining to narrative structure, such as the role of Narrator. A separate solution is proposed for treating conventionalized uses occurring outside speech and attitude reports.
Evidentials and their pivot in Tibetic and neighboring Himalayan languages
Authors: Zemp Marius 
Published: February 2020

AbstractThis paper focuses on a specific type of perspective-indexing constructions in Tibetic and neighboring languages, namely a type of verbal marker that is consistently construed from the perspective of the speaker in statements, the addressee in questions, and the source (= the original/reported speaker) in reported speech clauses. As these markers indicate how one obtained the information profiled in a sentence and may thus be viewed as a type of evidential, they cannot at the same time establish reference to any participant of the current speech act and thus by default reflect the perspective of the ‘informant’ of the respective sentence type. If we define the encountered distinctions in relation to a cause-effect vector in the sense ofDeLancey (1986), these languages all contain what we may call an ‘insider’ marker indicating access to the entire vector including its causal origin and an ‘outsider’ marker indicating access only to its effect end. Whereas the insider markers typically occur when the informant is the subject and the outsider markers when s/he is not, the present paper discusses the different ways in which Tibetic and neighboring languages deviate from this basic pattern, and argues that these differences reflect the fact that the markers in the latter languages were only secondarily evidentialized in reported speech clauses, likely due to contact with Tibetic.
Argumentation and meaning
Authors: Oswald Steve , Greco Sara , Miecznikowski Johanna , Pollaroli Chiara , Rocci Andrea 
Published: February 2020

Abstract
This special issue aims to explore the semantic and pragmatic dimensions of meaning in terms of their significance and relevance in the study of argumentation. Accordingly, the contributors to the project, who have all presented their work during the 2nd Argumentation and Language conference, which took place in Lugano in February 2018,1 have been specifically instructed to produce papers which explicitly tackle the importance of the study of meaning for that of argumentative practices. All papers therefore cover at least one aspect of this complex relationship between argumentation and meaning, which contributes to delivering a state-of-the-art panorama on the issue. Drawing from computational linguistics, semantics, pragmatics and discourse analysis, the contributions to this special issue will illuminate how the study of meaning in its different forms may provide valuable insights for the study of people’s argumentative practices in different contexts, ranging from the political to the private sphere. This introductory discussion tackles specific aspects of the intricate relationship between pragmatic inference and argumentative inference – that is, between meaning and argumentation –, provides a brief survey of existing interfaces between the study of meaning and that of argumentation, and concludes with a presentation of the contributions to this special issue.