bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of a Novel Immune Landscape Signature for Predicting Prognosis and Response of Endometrial Carcinoma to Immunotherapy and Chemotherapy
Authors: Liu Jinhui , Wang Yichun , Mei Jie , Nie Sipei , Zhang Yan 
Published: December 2020

Abstract
Background: Uterine Corpus Endometrial Carcinoma (UCEC) is the most common gynecological cancer. Here, we have investigated the significance of immune-related genes in predicting the prognosis and response of UCEC patients to immunotherapy and chemotherapy.Methods: Based on TCGA database, the single-sample gene-set enrichment analysis (ssGSEA scores) was utilized to obtain enrichment of 29 immune signatures. Univariate, multivariate Cox regression and LASSO regression analyses were performed to generate an immune-related prognostic signature (IRPS). The biological functions of IRPS-associated genes were evaluated using GSEA, TIMER Database analysis, Mutation analysis, IPS analysis, Gene Expression Profiling Interactive Analysis (GEPIA), Genomics of Drug Sensitivity in Cancer (GDSC) and Immune Cell Abundance Identifier (ImmuCellAI). Potential small molecule drugs for UCEC were predicted using the connectivity map (Cmap). The mRNA and protein expression levels of IRPS-associated genes were tested via quantitative real-time PCR (qPCR) and immunohistology.Results: Two immune-related genes (CCL13 and KLRC1) were identified to construct the IRPS. Both genes were related to the prognosis of UCEC patients (P<0.05). The IRPS could distinguish patients with different prognosis and was closely associated with the infiltration of several types of immune cells. Our findings showed that patients with low IRPS benefited more from immunotherapy and developed stronger response to several chemotherapies, which was also confirmed by the results of ImmuCellAI. Finally, we identified three small molecular drugs that might improve the prognosis of patients with high IRPS. Conclusion: IRPS can be utilized to predict the prognosis of UCEC patients and provide valuable information about their therapeutic response to immunotherapy and chemotherapy.
The Effects of Acid Etching on the Morphology and Properties of Medical Grade Alumina and Zirconia Surfaces for Prosthetics
Authors: Nakonieczny Damian S , Paszenda Zbigniew Kazimierz , Hundáková Marianna Hundáková , Kratošová Gabriela , Holešová Sylva , Majewska Justyna , Kałużyński Piotr , Martynková Gražyna Simha 
Published: December 2020

Abstract
In this study we modify and functionalize the surface of alumina and zirconia ceramics for medical applications using chemical etching with mixtures of sulfuric, nitric, hydrofluoric acids and peroxide. After etching, the impact of processes on surface development, chemical composition, and topography is studied to select the most effective process of surface development. Medical grade alumina and zirconia ceramic powders have been chemically etched with selected three kinds of acidic solutions : 1.sulfuric and nitric acid, 2.sulfuric acid and peroxide, 3.fluoric acid various diluted aqueous solution during the selected time periods. Following heat treatment was performed and the samples characterization were undertaken: morphology and chemical composition , phase composition, functional group determination, and the specific surface area and porosity evaluation.. Comparing the results raised from acidic etching, it was noticed that the use of H2SO4:HNO3 solutions causes sulphur residues in ceramic in the form of sulphates. The application of HF negatively affects the structure of the material and cause agglomeration. The most advantageous modification of ceramic powders was application of piranha solution, the obtaining surface development was achieved, satisfactory degree of agglomeration and post-process pollution.
Management of multiple rib fractures-results from a Major Trauma Centre with review of the existing literature
Authors: Chowdhury Debkumar , Okoh P. , Dambappa H. 
Published: December 2020

Abstract
Introduction Rib fractures are amongst the most common fractures following major trauma presenting to the Emergency Department. It accounts for more than 15% of ED presentations (1) on a global scale. As the population ages the incidence of rib fractures also rises often following falls from a relatively small height being part of fragility fractures. The impact of rib fractures is even more significant in the patient with underlying chronic respiratory conditions.Aim To assess our current management of rib fractures at our trauma centreMethod We collected our data from the TARN Registry primarily focussing on patients with multiple rib fractures. The main components were the analgesic requirement of our patients. We also studied the number of rib fracture stabilisation procedures and the average number of ribs fixed.Results The data was collected retrospectively over a period of 12 months. There were 313 patients identified as having chest wall injuries. From the data, 41.9% (131) of patients were over the age of 65 years. A significant proportion of our patients 34.5% (108) were noted to have multiple rib fractures (>3 Ribs). It was noted that 3% (9) of the 313 patients required operations. The average of the patients included in the study was noted to be 61 years with an age range of 17-92 years.Conclusion The mainstay management of rib fracture is provision of adequate analgesia and the prevention of respiratory complications that can all stem from poor ventilatory function amongst other patient factors and injury patterns. Through the decades, surgical stabilisation has gained pace and has found its niche in the management of rib fractures.
Introducing V-line as a New Strategy to Choose Surgical Corridor in Oblique Lumbar Interbody Fusion at the L5-S1 Segment
Authors: Zhang Wei , Du Xing , Zhu Yong , Luo Wei , Wang Ben , Jiang Guanyin , Ou Yunsheng 
Published: December 2020

Abstract
Purpose: To assess the availability of oblique lumbar interbody fusion at the level of L5-S1 (OLIF51) and to choose ideal surgical corridor in OLIF51 by introducing V-line. Methods: The axial views through the center of L5-S1 disc were reviewed. We adopt 18mm as the width of the simulated surgical corridor. The midline of the surgical corridor is at the center of L5-S1 disc. According to the traction distance of the left iliac vein (LCIV) and psoas major (PM), we defined all the subjects as V (+) (traction-difficultly LCIV), V (-) (traction-friendly LCIV), P (+) (traction-difficultly PM) and P (-) (traction-friendly PM). V-line was defined as a straight line dividing equally the simulated surgical corridor. All cases were divided into 2 groups: The V-line (+) group, more than half of the LCIV region is located in ventral part of V-line; the V-line (-) group, more than half of the LCIV region is located in dorsal part of V-line. Multiple variables regressive analysis was conducted to analyze the independent risk factors of V-line (+). Results: V-line (+) was found in 36 (38.7%) patients and V-line (-) in 57 (61.3%). Incidence of V (+) and P (+) were 35.4% (33/93) and 30.1% (28/93), respectively. 16.1% (15/93) subjects processed V (+) and P (+) at the same time. The independent risk factor of V-line (+) were gender of male (P = 0.034, OR: 12.152) and medial position of LCIV (P ＜ 0.001, OR: 265.085). High iliac crest was a significant independent protective factor (P = 0.001, OR: 0.750). Conclusions: Most patients were suitable for OLIF51. V-line could assess the injury risk of LCIV. Among male patients having the LCIV near the midline or the iliac crest relatively low, a surgical corridor external to the LCIV should be taken into consideration.
Increased Cerebrospinal Fluid Adenosine 5'-triphosphate in Patients with Amyotrophic Lateral Sclerosis
Authors: Nukui Takamasa , Matsui Atsushi , Niimi Hideki , Hayashi Tomohiro , Dougu Nobuhiro , Konishi Hirofumi , Yamamoto Mamoru , Anada Ryoko , Matsuda Noriyuki , Kitajima Isao , Nakatsuji Yuji 
Published: December 2020

Abstract
Background: Extracellular adenosine 5'-triphosphate (ATP) has been suggested to cause neuroinflammation and motor neuron degeneration by activating microglia and astrocytes in amyotrophic lateral sclerosis (ALS). Since we have developed a highly sensitive ATP assay system, we examined CSF ATP levels in patients with ALS whether it can be a useful biomarker in ALS. Methods: Forty-eight CSF samples from 45 patients with ALS were assayed for ATP with a newly established, highly sensitive assay system using luciferase luminous reaction. CSF samples from patients with idiopathic normal pressure hydrocephalus (iNPH) were assayed as a control. Patients were divided into two groups depending on their disease severity, as evaluated using the Medical Research Council (MRC) sum score. Correlations between the CSF ATP levels and other factors, including clinical data and serum creatinine levels, were evaluated. Results: ALSFRS-R (37.9 ± 5.7 vs. 42.4 ± 2.8, p<0.01) and serum creatinine levels (0.51 ± 0.13 vs. 0.68 ± 0.23 mg/dL, p < 0.05) were significantly lower in the severe group than in the mild group respectively. CSF ATP levels were significantly higher in the more severe group than in the iNPH group (6860 ± 8312 vs. 716 ± 411 pmol/L, p < 0.05) and mild group (6860 ± 8312 vs. 2676 ± 3959 pmol/L, p < 0.05) respectively. A negative correlation of CSF ATP levels with MRC sum score was demonstrated in the correlation analysis (r = -0.3862, p < 0.01). Conclusions: Extracellular ATP is particularly increased in the CSF of patients with advanced ALS. CSF ATP levels may be a useful biomarker for evaluating disease severity in patients with ALS.
Information Asymmetry between Physicians and Patients Undergoing Laparoscopic Cholecystectomy: Analysis of Patients’ Awareness Level
Authors: Jeddi Fatemeh Rangraz , Jabali Monireh Sadeqi , Tadayon Hamidreza , Khanmohammadi Mohammad Taghi 
Published: December 2020

Abstract
Background: Obtaining informed consent is essential before conducting a healthcare intervention. Primary reason for obtaining informed consent is to eliminate asymmetry information between the physicians and patients. The aim of this study was to determine the significance of information symmetry between physicians and patients undergoing laparoscopic cholecystectomy.Methods: An analytical study was carried out in 2019. Moreover, the research population consisted of patients undergoing laparoscopic cholecystectomy. As for the data collection tool, a researcher-made questionnaire was introduced. The validity and reliability of this questionnaire was confirmed. Questionnaire had five sections and Cronbach's alpha of 0.85 was achieved. The data were then analyzed by SPSS Version: 18.0.Results: A total of 96 patients participated in the study, where around 30% of them were male. Only 20.3% of patients specified that they were aware of possible complications of their treatment, of which 62% cited their physician as the main source of this information. On the other hand, 80% of patients were fully aware of the benefits of their treatment.Conclusions: The information asymmetry between physicians and patients about the benefits and complications of treatments should be somehow eliminated and the crucial information about the chosen treatment plan or alternative courses of action should be fully provided to the patient by the attending physician or nurse.
Observation of strong helical excitons and unexpectedly giant Rashba splitting in single crystal two-dimensional Organic-Inorganic Halide Perovskites
Authors: Rusydi Andrivo , Chi Xiao , Leng Kai , Li Runlai , Yu Xiaojiang , Yang Ping , Breese Mark , Loh Kian Ping 
Published: December 2020

Abstract
Two-dimensional (2D) organic-inorganic lead halide perovskites possess strong spin-orbit coupling, and in the presence of broken inversion symmetry, this may lead to helical excitons and Rashba splitting at the band extrema1-6. However, a direct and systematic measurement of the helical excitons and the Rashba parameter in the system is still lacking. Here, we report distinct two bright co-helical and two dark anti-helical excitons in single crystal ((CH3(CH2)3NH3)2(CH3NH3)n-1PbnI3n+1 (n = 1 to 4), where n determines quantum well (QW) thickness of inorganic layer7. By comprehensively analyzing the fine structure of helical excitons, we find that the Rashba splitting originates from surface inversion asymmetry and surface-normal electric fields, which are determined by the QW’s dielectric environment, n and temperature. The Rashba splitting parameter is found among the highest recorded of 2.66 and 2.5 eV∙Å for the conduction and valence band of n=1 Iodide, respectively, and reduces for n>1 Iodide. Our result shows the importance of helical excitons and Rashba splitting and presents a direct method to quantify the Rashba parameter in complex halide perovskites. We hope that our study inspires applications of QW materials in novel spintronic devices.
Grain Quality Characteristics and RVA&nbsp;Profile Analysis of Yuenongsimiao, a High Yield and Disease-resistant Rice Variety
Authors: Lu Zhanhua , Fang Zhiqiang , Liu Wei , Lu Dongbai , Wang Xiaofei , Wang Shiguang , Xue Jiao , He Xiuying 
Published: December 2020

Abstract
Background: Rice quality is one of the main targets for rice breeding, and is a complex trait involving grain appearance, milling, cooking, eating and nutritional quality. For a long time, rice breeding is facing the imbalance of yield, quality, disease resistance and lodging.Result: Here, the grain quality characters (milling and appearance quality, cooking quality, starch RVA profile, and nutritional quality) of Yuenongsimiao (YNSM), a new indica rice variety with high yield, high quality and disease resistance, were performed. It showed that YNSM has excellent appearance and quality with low amylose content, long gel consistency, and had significant correlation with the HPU, CPV, SB, and CS. Moreover, 5 genes related to grain size (GS3, GS9, GW5, GW7, and GW8) and Wx gene were selected to detect the main quality genotype of YNSM. The results showed YNSM was a middle-long grains with relative high brown rice rate, white rice rate and whole white rice rate, and lower chalkiness. The results indicated the grain size and food quality of YNSM might related to gs3, gw7 and Wxb. This research also reported the quality characters of hybrid rice using YNSM as a restorer lines.Conclusions: The quality characters and genotype of grain quality gene analysis in YNSM may facilitate the breeding of new rice varieties achieving a balance between grain yield, resistance and quality.
False Memory in Borderline Personality Disorder
Authors: Sajjadi Fatemeh , Sellbom Martin , Gross Julien , Hayne Harlene 
Published: December 2020

Abstract
Background: Borderline Personality Disorder (BPD) is a mental disorder characterized by significant impairment in intrapersonal and interpersonal functioning, as well as patterns of personality pathology. Memory deficits are not recognized as a core symptom of BPD, but BPD patients have long been suspected to have inaccurate perceptions, disturbed memory processes, and an increased tendency to develop false memories. Methods: In the present study, we examined whether there was an association between BPD and the production of false memories in the Deese-Roediger-McDermott (DRM) paradigm—a laboratory-based procedure that is frequently used to investigate false memory. We also compared the traditional and alternative model of BPD with respect to false memory. A total of 298 university students completed the McLean Screening Instrument for Borderline Personality Disorder, SCID-II Personality Questionnaire, Borderline Personality Disorder Impairment Scale, Personality Inventory for DSM-5, Beck Depression Inventory, Dissociative Experiences Scale, and Traumatic Life Events Questionnaire. The participants were also tested using both traditional DRM word lists as well as word lists that were specifically associated with BPD. Results: Using the traditional diagnosis of BPD, BPD symptoms were correlated with higher false memory for positive information; in the alternative model of BPD, identity impairment and anxiousness were correlated with the overall false memory score. We also found that trauma and dissociation mediated the relation between BPD and false memory. Conclusions: These findings are discussed in terms of how the consequences of trauma, such as dissociation and identity disturbance, are associated with false memory. Whether false memory rates are higher in a clinical population of patients with BPD remains to be determined.
New Strategy to Calculate Robust Impedance using RMHHT Estimator for Continuous and Discontinuous Broadband Magnetotelluric Time-Series Data
Authors: Chen Hao , Mizunaga Hideki , Tanaka Toshiaki , Wang Gang 
Published: December 2020

Abstract
Magnetotelluric (MT) method is an electromagnetic geophysical method for inferring the earth's subsurface electrical conductivity from measurements of natural geomagnetic and geoelectric field variation at the earth's surface. MT method is widely used in exploration surveys worldwide, but it was hardly applicable in urban areas because of large artificial electromagnetic noise. But in one day, there are several hours at midnight that most of the electric equipment is shutdown. The MT time-series data at midnight is much quiet than in the daytime. Therefore, we focused on calculating the MT impedance using the quiet time-series data. In this research, the data observed by the Phoenix System is used. We introduced a robust impedance estimator based on the Hilbert-Huang transform (RMHHT) and used a new strategy to calculate the broadband MT time-series data. We indicated that this technique needs 4-hour time-series data to get a reliable resistivity structure up to 1,000 seconds in the numerical simulation. This short measurement time makes it possible to carry out MT surveys in urban areas with strong noises. However, the biggest problem of the impedance estimator based on HHT is time-consuming. The total computation time can be reduced significantly by using this strategy. Finally, a successful case study using the midnight time-series data was demonstrated to get reliable resistivity structures in the areas contaminated by heavy noises.
Genome-wide identification and characterization of long non-coding RNAs in Longissimus dorsi skeletal muscle of Shandong black cattle and Luxi cattle
Authors: Liu Ruili , Liu Xianxun , Yu Kun , Bai Xuejin , Dong Yajuan 
Published: December 2020

Abstract
Background

There is increasing understanding of the possible regulatory role of long non-coding RNAs (LncRNA). Studies on livestock have mainly focused on the regulation of cell differentiation, fat synthesis, and embryonic development. However, there has been little study of skeletal muscle of domestic animals and the potential role of lncRNA.
Results

RNA samples were collected from longissimus dorsi muscle samples of Shandong black cattle and Luxi cattle and libraries were constructed and sequenced. A total of 1415 transcripts (of which 480 were LncRNAs) were differentially expressed (P < 0.05) in the different breeds, and fourteen of these RNAs were randomly selected and validated by qPCR. We found that the most differentially expressed LncRNAs were found on chromosome 9, with 1164 within 50 kb of a protein-coding gene. In addition, Pearson's correlation coefficients of co-expression levels indicated a potential trans regulatory relationship between the differentially expressed LncRNAs and 43844 mRNAs (r > 0.9). The identified co-expressed mRNAs (MYORG, Dll1, EFNB2, SOX6, MYOCD, and MYLK3) are related to the formation of muscle structure, and enriched in muscle system process, strained muscle cell differentiation, muscle cell development, striated muscle tissue development, calcium signaling, and AMPK signaling. Additionally, we also found that some LncRNAs (LOC112444238, LOC101903367, LOC104975788, LOC112441863, LOC112449549, and LOC101907194) may interact with miRNAs related to cattle muscle growth and development. Based on this, we constructed a LncRNAs-miRNA-mRNA interaction network as the putative basis for biological regulation in cattle skeletal muscle. Interestingly, a candidate differential LncRNA (LOC104975788) and a protein-coding gene (Pax7) contain miR-133a binding sites and binding was confirmed by luciferase reporter assay. LOC104975788 may bind miR-133a competitively with Pax7, thus relieving the inhibitory effect of miR-133a on Pax7 to regulate skeletal muscle development. These results will provide the theoretical basis for further study of LncRNA regulation and activity in different cattle breeds.
Conclusions

The data obtained in this study were used to predict muscle-related LncRNAs-miRNA-mRNA interaction networks, which can help elucidate the molecular mechanism of cattle muscle development. These results can be used to facilitate livestock breeding and improve livestock production.
A New Comprehensive Oral Health Literacy Scale: Development and Psychometric Evaluation
Authors: Sun Yue , Sun Jing , Zhao Yan , Cheng Aixiao , Zhou Junhong 
Published: December 2020

Abstract
Background: It has been widely accepted that oral health status is related to oral health literacy. The need to measure oral health literacy has led to the development of measurement instruments. This study aimed to develop a comprehensive instrument for adults and to examine its reliability and validity in China.Methods: A three-step design process was used. First, a literature review and expert panel discussion were used to draw up a 37-item pool covering oral health knowledge, belief, practice, skill and functional oral health literacy. The Delphi method was used to delete and modify questions in the item pool. The draft instrument was evaluated by nine experts and the consensus among them was calculated using the content validity index. The scale was then used to conduct a psychometric study among 370 participants from community health centers in Beijing. Construct validity, discriminant validity and concurrent validity were examined. The Cronbach’s alpha coefficient, and test–retest methods were used to assess reliability.Results: The final scale included 30 items in four dimensions. The item–level content validity index was 0.90. Exploratory factor analysis extracted four fixed factors, and the Kaiser–Meyer–Olkin and Bartlett’s tests came to 0.752, with the model explaining 35.21% of the total variance. The four dimensions were associated with oral health knowledge, perceptions of oral health issues, oral health practice and skills, and functional oral health literacy. The mean score of the lowest 27% was significantly lower than the highest 27% (p < 0.01), suggesting adequate discriminant validity. The associations between comprehensive oral health literacy scores and educational level, income and self-reported literacy level were significant (P < 0.001), showing adequate overall concurrent validity. Internal consistency and test–retest reliability were acceptable, with a Cronbach’s alpha of 0.72 and a total test–retest reliability coefficient of 0.979. Conclusions: Initial testing of the comprehensive oral health literacy instrument suggested that it is a valid and reliable instrument to evaluate individuals’ oral health literacy, with four dimensions for evaluating knowledge, belief, skill and functional oral health literacy.