bluuebunny/crossref_metadata_2025_split huggingface dataset:
The distinctive roles of urban community health workers in low- and middle-income countries: a scoping review of the literature
Authors: Ludwick Teralynn , Morgan Alison , Kane Sumit , Kelaher Margaret , McPake Barbara 
Published: May 2020

AbstractAddressing urban health challenges in low- and middle-income countries (LMICs) has been hampered by lack of evidence on effective mechanisms for delivering health services to the poor. The urban disadvantaged experience poor health outcomes (often worse than rural counterparts) and face service barriers. While community health workers (CHWs) have been extensively employed in rural communities to address inequities, little attention has been given to understanding the roles of CHWs in urban contexts. This study is the first to systematically examine urban CHW roles in LMICs. It aims to understand their roles vis-à-vis other health providers and raise considerations for informing future scope of practice and service delivery models. We developed a framework that presents seven key roles performed by urban CHWs and position these roles against a continuum of technical to political functions. Our scoping review included publications from four databases (MEDLINE, EMBASE, CINAHL and Social Sciences Citation Index) and two CHW resource hubs. We included all peer-reviewed, CHW studies situated in urban/peri-urban, LMIC contexts. We identify roles (un)commonly performed by urban CHWs, present the range of evidence available on CHW effectiveness in performing each role and identify considerations for informing future roles. Of 856 articles, 160 met the inclusion criteria. Programmes spanned 34 LMICs. Studies most commonly reported evidence on CHWs roles related to health education, outreach and elements of direct service provision. We found little overlap in roles between CHWs and other providers, with some exceptions. Reported roles were biased towards home visiting and individual-capacity building, and not well-oriented to reach men/youth/working women, support community empowerment or link with social services. Urban-specific adaptations to roles, such as peer outreach to high-risk, stigmatized communities, were limited. Innovation in urban CHW roles and a better understanding of the unique opportunities presented by urban settings is needed to fully capitalize on their potential.
Assessing County-Level Vulnerability for Opioid Overdose and Rapid Spread of Human Immunodeficiency Virus and Hepatitis C Infection in South Dakota
Authors: Wesner Chelsea A , Zhang Weiwei , Melstad Sandra , Ruen Elizabeth , Deffenbaugh Cassandra , Gu Wei , Clayton Joshua L 
Published: May 2020

Abstract

Background
Key indicators of vulnerability for the syndemic of opioid overdose, human immunodeficiency virus (HIV), and hepatitis C virus (HCV) due to injection drug use (IDU) in rural reservation and frontier counties are unknown. We examined county-level vulnerability for this syndemic in South Dakota.

Methods
Informed by prior methodology from the Centers for Disease Control and Prevention, we used acute and chronic HCV infections among persons aged ≤40 years as a proxy measure of IDU. Twenty-nine county-level indicators potentially associated with HCV infection rates were identified. Using these indicators, we examined relationships through bivariate and multivariate analysis and calculated a composite index score to identify the most vulnerable counties (top 20%) to this syndemic.

Results
Of the most vulnerable counties, 69% are reservation counties and 62% are rural. The county-level HCV infection rate is 4 times higher in minority counties than nonminority counties, and almost all significant indicators of opioid-related vulnerability in our analysis are structural and potentially modifiable through public health interventions and policies.

Conclusions
Our assessment gives context to the magnitude of this syndemic in rural reservation and frontier counties and should inform the strategic allocation of prevention and intervention services.
In vitro activity of sulbactam/durlobactam against global isolates of carbapenem-resistant Acinetobacter baumannii
Authors: Seifert Harald , Müller Carina , Stefanik Danuta , Higgins Paul G , Miller Alita , Kresken Michael 
Published: May 2020

AbstractObjectivesTo evaluate the activity of the novel broad-spectrum serine β-lactamase inhibitor durlobactam (ETX2514) combined with sulbactam against global isolates of carbapenem-resistant Acinetobacter baumannii with defined carbapenem resistance mechanisms compared with reference antimicrobials with known activity against Acinetobacter spp.MethodsThe susceptibility of 246 carbapenem-resistant non-duplicate A. baumannii isolates to sulbactam/durlobactam, amikacin, colistin, imipenem/sulbactam/durlobactam, imipenem, meropenem, minocycline and sulbactam was tested using broth microdilution. Isolates were obtained from various body sites from patients in 37 countries and from six world regions between 2012 and 2016. Identification of carbapenem resistance mechanisms and assignment to A. baumannii clonal lineages was based on WGS.ResultsSulbactam/durlobactam showed excellent activity comparable to colistin but superior to amikacin, minocycline and sulbactam. The sulbactam/durlobactam MIC50/90 values were 1/4 and 2/4 mg/L and the colistin MIC50/90 values were 0.5 and 1 mg/L, respectively. Comparatively, amikacin, minocycline and sulbactam MIC50/90 values were 256/≥512, 2/16 and 16/64 mg/L, respectively.ConclusionsSulbactam/durlobactam had excellent in vitro potency against A. baumannii isolates, including those that were resistant to imipenem/meropenem, amikacin, minocycline and colistin, compared with other compounds. Sulbactam/durlobactam has the potential to become a useful addition to the limited armamentarium of drugs that can be used to treat this problem pathogen.
Patient safety and quality improvement: Ethical principles for a regulatory approach to bias in healthcare machine learning
Authors: McCradden Melissa D , Joshi Shalmali , Anderson James A , Mazwi Mjaye , Goldenberg Anna , Zlotnik Shaul Randi 
Published: May 2020

Abstract
Accumulating evidence demonstrates the impact of bias that reflects social inequality on the performance of machine learning (ML) models in health care. Given their intended placement within healthcare decision making more broadly, ML tools require attention to adequately quantify the impact of bias and reduce its potential to exacerbate inequalities. We suggest that taking a patient safety and quality improvement approach to bias can support the quantification of bias-related effects on ML. Drawing from the ethical principles underpinning these approaches, we argue that patient safety and quality improvement lenses support the quantification of relevant performance metrics, in order to minimize harm while promoting accountability, justice, and transparency. We identify specific methods for operationalizing these principles with the goal of attending to bias to support better decision making in light of controllable and uncontrollable factors.
Detection of Paralytic Shellfish Toxins in Southern Rock Lobster Jasus edwardsii Using the Qualitative Neogen™ Lateral Flow Immunoassay: Single-Laboratory Validation
Authors: Dorantes-Aranda Juan José , Hayashi Aiko , Turnbull Alison R , Jolley Jessica Y C , Harwood D Tim , Hallegraeff Gustaaf M 
Published: May 2020

Abstract

Background
Paralytic shellfish toxins (PST) are a significant problem for the Tasmanian shellfish and Southern Rock Lobster (Jasus edwardsii) industries, and the introduction of a rapid screening test in the monitoring program could save time and money.

Objective
The aim was to perform a single-laboratory validation of the Neogen rapid test for PST in the hepatopancreas of Southern Rock Lobster.

Methods
The AOAC INTERNATIONAL guidelines for the validation of qualitative binary chemistry methods were followed. Three different PST profiles (mixtures) were used, of which two were commonly found in naturally contaminated lobster hepatopancreas (high in gonyautoxin 2&3 and saxitoxin), and the third toxin profile was observed in a few select animals (high in gonyautoxin 1&4).

Results
The Neogen test consistently returned negative results for non-target toxins (selectivity). The probability of detection (POD) of PST in the lobster hepatopancreas using the Neogen test increased with increasing PST concentrations. POD values of 1.0 were obtained at ≥0.57 mg STX-diHCl eq/kg in mixtures 1 and 2, and 0.95 and 1.0 for mixture 3 at 0.79 and 1.21 mg STX-diHCl eq/kg, respectively, with a fitted POD of 0.98 for 0.80 mg STX-diHCl eq/kg. The performance of the Neogen test when using four different production lots (ruggedness) showed no significant differences.

Conclusions
The results of the validation study were satisfactory and the Neogen test is being trialed within the Tasmanian PST monitoring program of Southern Rock Lobster.

Highlights
The Neogen rapid kit was successfully validated for the detection of PST in Southern Rock Lobster hepatopancreas.
Site-specific epitope insertion into recombinant proteins using the MAP tag system
Authors: Wakasa Ayami , Kaneko Mika K , Kato Yukinari , Takagi Junichi , Arimori Takao 
Published: May 2020

AbstractThe MAP tag system comprises a 14-residue peptide derived from mouse podoplanin and its high-affinity monoclonal antibody PMab-1. We determined the crystal structure of PMab-1 complexed with the MAP tag peptide and found that the recognition required only the N-terminal 8 residues of MAP tag sequence, enabling the shortening of the tag length without losing the affinity for PMab-1. Furthermore, the structure illustrated that the MAP tag adopts a U-shaped conformation when bound by PMab-1, suggesting that loop-inserted MAP tag would assume conformation compatible with the PMab-1 binding. We inserted the 8-residue MAP tag into multiple loop regions in various proteins including fibronectin type III domain and G-protein-coupled receptors and tested if they maintain PMab-1 reactivity. Despite the conformational restraints forced by the insertion position, all MAP-inserted mutants were expressed well in mammalian cells at levels comparable to the non-tagged proteins. Furthermore, the binding by PMab-1 was fully maintained even for the mutant where MAP tag was inserted at a structurally restricted β-hairpin, indicating that the MAP tag system has unique feature that allows placement in the middle of protein domain at desired locations. Our results indicate the versatile utility of the MAP tag system in ‘site-specific epitope insertion’ application.
International Economic Law and Disintegration: Beware the Schmittean Moment
Authors: Arcuri Alessandra 
Published: May 2020

Abstract
In his influential book, Straight Talk on Trade, Dani Rodrik provides a cogent critique of the existing international economic order and concludes as follows: ‘So, I accept that nation-states are a source of disintegration for the global economy.’ This article critically engages with the idea that the nation-state is a legitimate force of disintegration of the international economic order, with particular attention to trade and investment agreements. In times of raising authoritarianism, it is crucial to reflect on some of the limits of the nation-state and on the necessity to develop alternative paradigms for integrating economies and societies. Against this background, this article posits that we should beware of the risk of a ‘Schmittean moment’. This term is used to refer to a major shift toward an ideal of unfettered national sovereignty as the chief paradigm to re-orient the international (economic) order. Under such ideal, any international normative benchmark is brushed away by an allegedly more intellectually honest ‘political’ dimension, which can find its realization only in the decisionist state. To understand the risk of a ‘Schmittean moment’ it is important to recognize that the move toward more nation-state is partly animated by some legitimate concerns over the existing international legal order, such as those underpinning the analysis by Dani Rodrik. This article articulates a two-fold critique of the idea that an expansion of national sovereignty is going to achieve a better socio-economic world order per se. The first critique is internal, showing that the nation-state does not possess intrinsic characteristics to facilitate democracy, equality, and sustainability. The second is external and focuses on the necessity to look reflexively at the goals of the system of international economic law, to re-imagine it as capable to address questions of inequality and environmental degradation.
The MUC6/AP2A2 Locus and Its Relevance to Alzheimer’s Disease: A Review
Authors: Nelson Peter T , Fardo David W , Katsumata Yuriko 
Published: May 2020

Abstract
We recently reported evidence of Alzheimer’s disease (AD)-linked genetic variation within the mucin 6 (MUC6) gene on chromosome 11p, nearby the adaptor-related protein complex 2 subunit alpha 2 (AP2A2) gene. This locus has interesting features related to human genomics and clinical research. MUC6 gene variants have been reported to potentially influence viral—including herpesvirus—immunity and the gut microbiome. Within the MUC6 gene is a unique variable number of tandem repeat (VNTR) region. We discovered an association between MUC6 VNTR repeat expansion and AD pathologic severity, particularly tau proteinopathy. Here, we review the relevant literature. The AD-linked VNTR polymorphism may also influence AP2A2 gene expression. AP2A2 encodes a polypeptide component of the adaptor protein complex, AP-2, which is involved in clathrin-coated vesicle function and was previously implicated in AD pathogenesis. To provide background information, we describe some key knowledge gaps in AD genetics research. The “missing/hidden heritability problem” of AD is highlighted. Extensive portions of the human genome, including the MUC6 VNTR, have not been thoroughly evaluated due to limitations of existing high-throughput sequencing technology. We present and discuss additional data, along with cautionary considerations, relevant to the hypothesis that MUC6 repeat expansion influences AD pathogenesis.
Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene
Authors: Mitalo Oscar W , Otsuki Takumi , Okada Rui , Obitsu Saeka , Masuda Kanae , Hojo Yuko , Matsuura Takakazu , Mori Izumi C , Abe Daigo , Asiche William O , Akagi Takashi , Kubo Yasutaka , Ushijima Koichiro 
Published: May 2020

Abstract
Peel degreening is an important aspect of fruit ripening in many citrus fruit, and previous studies have shown that it can be advanced by ethylene treatment or by low-temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To determine how natural peel degreening is regulated in lemon fruit (Citrus limon), we studied transcriptome and physiochemical changes in the flavedo in response to ethylene treatment and low temperatures. Treatment with ethylene induced rapid peel degreening, which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25 ºC, moderately low storage temperatures of 5–20 °C also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. Peel degreening of fruit on trees occurred in association with drops in ambient temperature, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit.
The Impact of Military Pediatrics: Assessing Clinical, Leadership, Academic, and Operational Experience Among Pediatric-trained Graduates From the Uniformed Services University of the Health Sciences (USU)
Authors: Judd Courtney A , Dong Ting , Meyer Holly S , Hickey Patrick W , Torre Dario M , Durning Steven J 
Published: May 2020

Abstract

Introduction
This article uses alumni survey data from the Uniformed Services University of the Health Sciences (USU) F. Edward Hébert School of Medicine to assess the professional impact of pediatric-trained USU graduates over the course of their careers. We specifically report on the clinical and leadership roles held, career accomplishments, and operational involvement among military pediatricians.

Materials and Methods
This study analyzed survey data that were collected from alumni of USU. We used descriptive statistics to report the career achievements and operational experiences among USU graduates who completed training in pediatrics. This study was deemed exempt by the USU Institutional Review Board.

Results
The survey response rate was 49.5% among 2,400 eligible respondents. Out of 1,189 alumni respondents, 110 (9.3%) trained in pediatrics. Among the pediatric-trained USU graduates, 98.2% spent some time as a full-time clinician, 73.6% served as chief of a clinical service, 42.7% held the role of department chair/chief or the equivalent, and 26.3% filled leadership positions in deployed settings. Forty percent of USU-trained military pediatricians deployed to combat areas at least once, and 35.5% participated in at least one peacetime humanitarian mission.

Conclusions
Our findings contribute to the unique story of military pediatricians who graduated from USU. These uniformed pediatricians participate actively in all realms of military medicine and have demonstrated their versatility through participation in a wide variety of vital functions. Their contributions include the provision of clinical care for both military children and active duty service members, serving in varied leadership positions, engaging in health professions education and other academic pursuits, and participating in operational assignments. Future studies could aim to more fully address the diverse contributions of military pediatricians to the overall mission by including more specific data about career experiences from all uniformed pediatricians.
A missing outskirts problem? Comparisons between stellar haloes in the Dragonfly Nearby Galaxies Survey and the TNG100 simulation
Authors: Merritt Allison , Pillepich Annalisa , van Dokkum Pieter , Nelson Dylan , Hernquist Lars , Marinacci Federico , Vogelsberger Mark 
Published: May 2020

ABSTRACT
Low surface brightness galactic stellar haloes provide a challenging but promising path towards unravelling the past assembly histories of individual galaxies. Here, we present detailed comparisons between the stellar haloes of Milky Way-mass disc galaxies observed as part of the Dragonfly Nearby Galaxies Survey (DNGS) and stellar mass-matched galaxies in the TNG100 run of the IllustrisTNG project. We produce stellar mass maps as well as mock g- and r-band images for randomly oriented simulated galaxies, convolving the latter with the Dragonfly point spread function (PSF) and taking care to match the background noise, surface brightness limits, and spatial resolution of DNGS. We measure azimuthally averaged stellar mass density and surface brightness profiles, and find that the DNGS galaxies generally have less stellar mass (or light) at large radii (&gt;20 kpc) compared to their mass-matched TNG100 counterparts, and that simulated galaxies with similar surface density profiles tend to have low accreted mass fractions for their stellar mass. We explore potential solutions to this apparent ‘missing outskirts problem’ by implementing several ad hoc adjustments within TNG100 at the stellar particle level. Although we are unable to identify any single adjustment that fully reconciles the differences between the observed and simulated galaxy outskirts, we find that artificially delaying the disruption of satellite galaxies and reducing the spatial extent of in-situ stellar populations result in improved matches between the outer profile shapes and stellar halo masses, respectively. Further insight can be achieved with higher resolution simulations that are able to better resolve satellite accretion, and with larger samples of observed galaxies.
Searching for globular cluster chemical anomalies on the main sequence of a young massive cluster
Authors: Cabrera-Ziri I , Speagle J S , Dalessandro E , Usher C , Bastian N , Salaris M , Martocchia S , Kozhurina-Platais V , Niederhofer F , Lardo C , Larsen S S , Saracino S 
Published: May 2020

ABSTRACT
The spectroscopic and photometric signals of the star-to-star abundance variations found in globular clusters seem to be correlated with global parameters like the cluster’s metallicity, mass, and age. Understanding this behaviour could bring us closer to the origin of these intriguing abundance spreads. In this work we use deep HST photometry to look for evidence of abundance variations in the main sequence of a young massive cluster NGC 419 (∼105 M⊙, ∼1.4 Gyr). Unlike previous studies, here we focus on stars in the same mass range found in old globulars (∼0.75–1 M⊙), where light elements variations are detected. We find no evidence for N abundance variations among these stars in the Un − B and U − B colour–magnitude diagrams of NGC 419. This is at odds with the N variations found in old globulars like 47 Tuc, NGC 6352, and NGC 6637 with similar metallicity to NGC 419. Although the signature of the abundance variations characteristic of old globulars appears to be significantly smaller or absent in this young cluster, we cannot conclude if this effect is mainly driven by its age or its mass.
A quasar microlensing light-curve generator for LSST
Authors: Neira Favio , Anguita Timo , Vernardos Georgios 
Published: May 2020

ABSTRACT
We present a tool to generate mock quasar microlensing light curves and sample them according to any observing strategy. An updated treatment of the fixed and random velocity components of observer, lens, and source is used, together with a proper alignment with the external shear defining the magnification map caustic orientation. Our tool produces quantitative results on high magnification events and caustic crossings, which we use to study three lensed quasars known to display microlensing, viz. RX J1131–1231, HE 0230–2130, and Q 2237+0305, as they would be monitored by The Rubin Observatory Legacy Survey of Space and Time (LSST). We conclude that depending on the location on the sky, the lens and source redshift, and the caustic network density, the microlensing variability may deviate significantly than the expected ∼20-yr average time-scale (Mosquera & Kochanek 2011). We estimate that ∼300 high magnification events with Δmag&gt;1 mag could potentially be observed by LSST each year. The duration of the majority of high magnification events is between 10 and 100 d, requiring a very high cadence to capture and resolve them. Uniform LSST observing strategies perform the best in recovering microlensing high magnification events. Our web tool can be extended to any instrument and observing strategy, and is freely available as a service at http://gerlumph.swin.edu.au/tools/lsst_generator/, along with all the related code.