bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential impact of seasonal forcing on a SARS-CoV-2 pandemic
Authors: Neher Richard A. , Dyrdak Robert , Druelle Valentin , Hodcroft Emma B. , Albert Jan 
Published: February 2020

A novel coronavirus (SARS-CoV-2) first detected in Wuhan, China, has spread rapidly since December 2019, causing more than 80,000 confirmed infections and 2,700 fatalities (as of Feb 27, 2020). Imported cases and transmission clusters of various sizes have been reported globally suggesting a pandemic is likely.Here, we explore how seasonal variation in transmissibility could modulate a SARS-CoV-2 pandemic. Data from routine diagnostics show a strong and consistent seasonal variation of the four endemic coronaviruses (229E, HKU1, NL63, OC43) and we parameterize our model for SARS-CoV-2 using these data. The model allows for many subpopulations of different size with variable parameters. Simulations of different scenarios show that plausible parameters result in a small peak in early 2020 in temperate regions of the Northern Hemisphere and a larger peak in winter 2020/2021. Variation in transmission and migration rates can result in substantial variation in prevalence between regions.While the uncertainty in parameters is large, the scenarios we explore show that transient reductions in the incidence rate might be due to a combination of seasonal variation and infection control efforts but do not necessarily mean the epidemic is contained. Seasonal forcing on SARS-CoV-2 should thus be taken into account in the further monitoring of the global transmission. The likely aggregated effect of seasonal variation, infection control measures, and transmission rate variation is a prolonged pandemic wave with lower prevalence at any given time, thereby providing a window of opportunity for better preparation of health care systems.
High-throughput mapping of the phage resistance landscape inE. coli
Authors: Mutalik Vivek K. , Adler Benjamin A. , Rishi Harneet S. , Piya Denish , Zhong Crystal , Koskella Britt , Calendar Richard , Novichkov Pavel , Price Morgan N. , Deutschbauer Adam M. , Arkin Adam P. 
Published: February 2020

AbstractBacteriophages (phages) are critical players in the dynamics and function of microbial communities and drive processes as diverse as global biogeochemical cycles and human health. Phages tend to be predators finely tuned to attack specific hosts, even down to the strain level, which in turn defend themselves using an array of mechanisms. However, to date, efforts to rapidly and comprehensively identify bacterial host factors important in phage infection and resistance have yet to be fully realized. Here, we globally map the host genetic determinants involved in resistance to 14 phylogenetically diverse double-stranded DNA phages using two modelEscherichia colistrains (K-12 and BL21) with known sequence divergence to demonstrate strain-specific differences. Using genome-wide loss-of-function and gain-of-function genetic technologies, we are able to confirm previously described phage receptors as well as uncover a number of previously unknown host factors that confer resistance to one or more of these phages. We uncover differences in resistance factors that strongly align with the susceptibility of K-12 and BL21 to specific phage. We also identify both phage specific mechanisms, such as the unexpected role of cyclic-di-GMP in host sensitivity to phage N4, and more generic defenses, such as the overproduction of colanic acid capsular polysaccharide that defends against a wide array of phages. Our results indicate that host responses to phages can occur via diverse cellular mechanisms. Our systematic and high-throughput genetic workflow to characterize phage-host interaction determinants can be extended to diverse bacteria to generate datasets that allow predictive models of how phage-mediated selection will shape bacterial phenotype and evolution. The results of this study and future efforts to map the phage resistance landscape will lead to new insights into the coevolution of hosts and their phage, which can ultimately be used to design better phage therapeutic treatments and tools for precision microbiome engineering.
Long non-coding RNA Gm12664 up-regulates CAV1 expression to promote hepatocellular lipid accumulation by sponging miR-295-5p
Authors: Xu Fei , Wang Liqiang , Chen Linjun , Zhang Qingwen , Na Lixin 
Published: February 2020

The authors have withdrawn this manuscript because the manuscript did not pass though the animal ethical review. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.Lixin Na
Pluripotency factors determine gene expression repertoire at zygotic genome activation
Authors: Gao Meijiang , Veil Marina , Rosenblatt Marcus , Riesle Aileen Julia , Gebhard Anna , Hass Helge , Buryanova Lenka , Yampolsky Lev Y. , Grüning Björn , Ulianov Sergey V. , Timmer Jens , Onichtchouk Daria 
Published: February 2020

AbstractAwakening of zygotic transcription in animal embryos relies on maternal pioneer transcription factors. The interplay of global and specific functions of these proteins remains poorly understood. Here, we analyze chromatin accessibility and time-resolved transcription in single and double mutant zebrafish embryos lacking pluripotency factors Pou5f3 and Sox19b. We show that two factors modify chromatin in a largely independent manner. We distinguish four types of direct enhancers by differential requirements for Pou5f3 or Sox19b. We demonstrate that changes in chromatin accessibility of enhancers underlie the changes in zygotic expression repertoire in the double mutants. Pou5f3 or Sox19b promote chromatin accessibility of enhancers linked to the genes involved in gastrulation and ventral fate specification. The genes regulating mesendodermal and dorsal fates are primed for activation independently of Pou5f3 and Sox19b. Strikingly, simultaneous loss of Pou5f3 and Sox19b leads to premature expression of genes, involved in regulation of organogenesis and differentiation.
Structure of the Lifeact–F-actin complex
Authors: Belyy Alexander , Merino Felipe , Sitsel Oleg , Raunser Stefan 
Published: February 2020

AbstractLifeact is a short actin-binding peptide that is used to visualize filamentous actin (F-actin) structures in live eukaryotic cells using fluorescence microscopy. However, this popular probe has been shown to alter cellular morphology by affecting the structure of the cytoskeleton. The molecular basis for such artefacts is poorly understood. Here, we determined the high-resolution structure of the Lifeact–F-actin complex using electron cryo-microscopy. The structure reveals that Lifeact interacts with a hydrophobic binding pocket on F-actin and stretches over two adjacent actin subunits, stabilizing the DNase I-binding loop of actin in the closed conformation. Interestingly, the hydrophobic binding site is also used by actin-binding proteins, such as cofilin and myosin and actin-binding toxins, such as TccC3HVR fromPhotorhabdus luminescensand ExoY fromPseudomonas aeruginosa.In vitrobinding assays and activity measurements demonstrate that Lifeact indeed competes with these proteins, providing an explanation for the altering effects of Lifeact on cell morphologyin vivo. Finally, we demonstrate that the affinity of Lifeact to F-actin can be increased by introducing mutations into the peptide, laying the foundation for designing improved actin probes for live cell imaging.
Eukaryotic rather than prokaryotic microbiomes change over seasons in rewetted fen peatlands
Authors: Wang Haitao , Weil Micha , Dumack Kenneth , Zak Dominik , Münch Diana , Günther Anke , Jurasinski Gerald , Blume-Werry Gesche , Kreyling Jürgen , Urich Tim 
Published: February 2020

AbstractIn the last decades, rewetting of drained peatlands is on the rise worldwide, to restore the significant carbon sink function. Rewetted peatlands differ substantially from their pristine counterparts and can, thus, be considered as novel ecosystems. Despite the increasing understanding of peat microbiomes, little is known about the seasonal dynamics and network interactions of the microbial communities in these novel ecosystems, especially in rewetted groundwater-fed peatlands, i.e. fens. Here, we investigated the seasonal dynamics in both prokaryotic and eukaryotic microbiomes in three common types of fens in Northern Germany, namely percolation fen, alder forest and coastal fen. The eukaryotic microbiomes, including fungi, protists and metazoa, showed significant changes of their community structures across the seasons in contrast to largely unaffected prokaryotic microbiomes. The co-occurrence network in the summer showed a distinct topology compared to networks in the other seasons, which was driven by the increased connections among protists, as well as between protists and the other microbial groups. Our results also indicated that the dynamics in eukaryotic microbiomes differed between fen types, specifically in terms of saprotrophs, arbuscular mycorrhiza and grazers of bacteria. Our study provides the insight that microbial eukaryotes mainly define the seasonal dynamics of microbiomes in rewetted fen peatlands. Accordingly, future research should unravel the importance of eukaryotes for biogeochemical processes, especially the under-characterized protists and metazoa, in these novel yet poorly understood ecosystems.
Gas evolution and the effects on ionic transport inside the lithium-ion battery
Authors: Li Na , Fu Lijun , Jiang Kecheng 
Published: February 2020

Purpose
Gas evolution within lithium-ion batteries (LIBs) gives rise to safety concerns that question their applicability. The gas evolution is not only the result but also the inducement of performance deterioration of LIBs. In this paper, the growth characteristics and dynamic behavior of gas bubble on the electrode surface are studied, and the interference mechanism of gas evolution on Li-ion diffusion or Li-ion conduction within LIBs is discussed and validated by the numerical simulations.

Design/methodology/approach
First, the mathematical models and simulation method are established. The growth and flow of gas bubble in the serpentine channel on electrode surface, which results from the gas-liquid flow and the effects of surface tension, is modeled by using the multi-phase Navier-Stokes and the volume of fluid method. Integrating Butler–Volmer and Fick’s law, the mathematical model of ions transport in the electrochemical cell is set-up. Second, the motion of gas bubble is tracked, and the variations of bubble shape and characteristic parameters with time are obtained by the computed fluid dynamics (CFD) method.

Findings
Based on the CFD results, the battery models and electrochemical simulations are carried out to analyze the ionic transport characteristics. The results show that the microstructural morphology such as the serpentine channel shape and size on electrode surface are important aspects for the gas bubble growth and the local ionic transport. Li ions significantly accumulate at one side of the gas obstacle, hindering the ionic diffusion normally. When the gas bubble blocks the electrolyte, the passage of ions from the positive to the negative is interrupted, and the open circuit zone of the electrochemical cell is formed.

Originality/value
The gas evolution within LIBs is not only a result but also an inducement of its performance deterioration. The primary issues in this study are the growth characteristics and dynamic behavior of gas bubble on the electrode surface, providing the knowledge for the interference mechanism of gas evolution on ionic transport and ultimately leads to significant increase of battery resistance.
Collective resistance to collective collaboration: a leader’s introspection
Authors: Sengupta Atri , Sonawane Bhaurao 
Published: February 2020

Learning outcomes
Ethical leadership and decision-making, engaging and managing multiple conflicting stakeholders.

Case overview/synopsis
Utkal Alumina International Limited (UAIL), an ambitious Greenfield project of Hindalco Industries Ltd. faced the challenges of conflicting multiple stakeholders and collective resistance that did not allow UAIL to be commissioned for almost two decades. The case portrayed the journey of a new leader of UAIL in the path of transforming collective resistance to collective collaboration. In the case, the author was introspecting about how far the author could achieve the objective of collective collaborations through stakeholders’ engagements and what would be the next path of the journey.

Complexity academic level
MBA and executive development programs.

Supplementary materials
Teaching Notes are available for educators only.

Subject code
CSS 6: Human Resources.
Consequences of open innovation: effects on skill-driven recruitment
Authors: Bello-Pintado Alejandro , Bianchi Carlos 
Published: February 2020

PurposeThis paper aims to focus on the human side of inbound open innovation by analyzing the effects that the adoption of different knowledge search strategies for innovation has on new recruitment needs.Design/methodology/approachBuilding on several theoretical perspectives, the study proposes three hypotheses regarding the relationship between openness and the need to recruit people with high technical and social skills. Using a pooled panel data from the Uruguayan Innovation Survey between 2004 and 2012, the authors identify open strategies followed by the firm.FindingsThe estimation results using pooled panel data confirm that the adoption of inbound open search strategies for innovation demands the recruitment of new employees with higher technical and social skills. Technical skills are more likely to be demanded than social skills. The effects observed are moderated by the intensity in the use of knowledge and information sources (KISs).Originality/valueThis paper revisits the analysis of specific knowledge search strategies at the firm level. In doing so, the study looks for the effects of specific strategies combining different knowledge sources and considers different levels of use of external KISs, from narrow to wide. While other studies have analyzed the human factor as a determinant of the success of openness for innovation, this paper re-examines the direction of this relationship. Finally, the study contributes to the evidence from a Latin American country, where these topics have received less attention.
Cooking a socially responsible image for the urban consumers: does it work with the cooking oil brands?
Authors: Banerjee Anulekha , Dasgupta Rajib 
Published: February 2020

PurposeThe consumer-based study was conducted among the population of Kolkata metropolis to assess the impact of corporate social responsibility (CSR) practices on the purchase intention of selected cooking oil brands.Design/methodology/approachData were collected from a questionnaire based survey on 322 respondents residing in Kolkata metropolis. Reliability of the scales was ascertained by Cronbach’s alpha values. Kendall's W test was used for rank analysis. Pearson’s correlation was examined to correlate the cognitive criteria. Factor analysis was used to sort out influential cognitive criteria which were compared between genders by the Kruskal–Wallis H test. The involvement of CSR components in enhancing the brand equity was analysed by multiple linear regression.FindingsThe brands vouching for the cause of health and nutritional value of the society attained significant loyalty and generate considerable brand association. The regression model predicts a socially accepted cooking oil brand to be one which addresses health, transparency and ethics in unison.Research limitations/implicationsThe study was restricted within the resident population of Kolkata metropolis which ratifies the CSR perception of a confined mass.Practical implicationsThe study delineates the plausible avenue of CSR investments to touch the cognitive centre of the consumers’ mind.Social implicationsThe consumers expect to embrace a healthy yet reasonably priced cooking oil brand which imparts a notion to address multiple social causes.Originality/valueThe study identifies the strategic CSR attributes which might influence the mind of the consumers while they select cooking oil brands for household use.
Geomagnetic field absence reduces adult body weight of a migratory insect by disrupting feeding behavior and appetite regulation
Authors: Wan Gui‐Jun , Jiang Shou‐Lin , Zhang Ming , Zhao Jing‐Yu , Zhang Ying‐Chao , Pan Wei‐Dong , Sword Gregory A. , Chen Fa‐Jun 
Published: February 2020

AbstractThe geomagnetic field (GMF) is well documented for its essential role as a cue used in animal orientation or navigation. Recent evidence indicates that the absence of GMF (mimicked by the near‐zero magnetic field, NZMF) can trigger stress‐like responses such as reduced body weight, as we have previously shown in the brown planthopper, Nilaparvata lugens. In this study, we found that consistent with the significantly decreased body weight of newly emerged female (−14.67%) and male (−13.17%) adult N. lugens, the duration of the phloem ingestion feeding waveform was significantly reduced by 32.02% in 5th instar nymphs reared under the NZMF versus GMF. Interestingly, 5th instar nymphs that exhibited reduced feeding had significantly higher glucose levels (+16.98% and +20.05%; 24 h and 48 h after molting), which are associated with food aversion, and expression patterns of their appetite‐related neuropeptide genes (neuropeptide F, down‐regulated overall; short neuropeptide F, down‐regulated overall; adipokinetic hormone, up‐regulated overall; and adipokinetic hormone receptor, down‐regulated overall) were also altered under the absence of GMF in a manner consistent with diminishing appetite. Moreover, the expressions of the potential magnetosensor cryptochromes (Crys) were found significantly altered under the absence of GMF, indicating the likely upstream signaling of the Cry‐mediated magnetoreception mechanisms. These findings support the hypothesis that strong changes in GMF intensity can reduce adult body weight through affecting insect feeding behavior and underlying regulatory processes including appetite regulation. Our results highlight that GMF could be necessary for the maintenance of energy homeostasis in insects.
Mosquitocidal and repellent activities of essential oils againstCulex pipiensL.
Authors: Al‐Sarar Ali S. , Hussein Hamdy I. , Abobakr Yasser , Al‐Zabib Ali A.S. , Bazeyad Abdulqader Y. 
Published: February 2020

AbstractPlants are a prospective source of novel natural insect repellents and botanical insecticides. This study was conducted to investigate the chemical composition of the essential oils of three plants growing in Saudi Arabia, namelyDucrosia anethifolia,Achillea fragrantissima, andTeucrium polium; and to evaluate their potential mosquitocidal and repellent activities against adult femaleCulex pipiensL. The main components of the three oils were found to be decanal (28.9%) and chrysanthenyl acetate (10.04%), (D. anethifolia); sabinyl acetate (35.79) and artemesia ketone (18.28%) (A. fragrantissima);α‐cadinol (49.53%) andδ‐cadinene (10.23%) (T. polium). The oil ofA. fragrantissimawas the most toxic (LC50 = 0.11 μL/L air) followed byD. anethifoliaandT. poliumwith LC50values of 5.22 and 25.98 μL/L air, respectively.T. poliumoil was the most repellent (292 min at 2 μL/cm2), followed byD. anethifoliaandA. fragrantissima. The results indicate that the essential oils have a potential fumigant insecticidal and repellent activities for mosquito control.
Streamlining universal single‐copy orthologue and ultraconserved element design: A case study in Collembola
Authors: Sun Xin , Ding Yinhuan , Orr Michael C. , Zhang Feng 
Published: February 2020

AbstractGenomic data sets are increasingly central to ecological and evolutionary biology, but far fewer resources are available for invertebrates. Powerful new computational tools and the rapidly decreasing cost of Illumina sequencing are beginning to change this, enabling rapid genome assembly and reference marker extraction. We have developed and tested a practical workflow for developing genomic resources in nonmodel groups with real‐world data on Collembola (springtails), one of the most dominant soil animals on Earth. We designed universal molecular marker sets, single‐copy orthologues (BUSCOs) and ultraconserved elements (UCEs), using three existing and 11 newly generated genomes. Both marker types were tested in silico via marker capture success and phylogenetic performance. The new genomes were assembled with Illumina short reads and 9,585‒14,743 protein‐coding genes were predicted with ab initio and protein homology evidence. We identified 1,997 benchmarking universal single‐copy orthologues (BUSCOs) across 14 genomes and created and assessed a custom BUSCO data set for extracting single‐copy genes. We also developed a new UCE probe set containing 46,087 baits targeting 1,885 loci. We successfully captured 1,437‒1,865 BUSCOs and 975‒1,186 UCEs across 14 genomes. Phylogenomic reconstructions using these markers proved robust, giving new insight on deep‐time collembolan relationships. Our study demonstrates the feasibility of generating thousands of universal markers from highly efficient whole‐genome sequencing, providing a valuable resource for genome‐scale investigations in evolutionary biology and ecology.
Efficacy of transcutaneous electrical acupoint stimulation combined with general anesthesia for sedation and postoperative analgesia in minimally invasive lung cancer surgery: A randomized, double‐blind, placebo‐controlled trial
Authors: Chen Jiheng , Zhang Yunxiao , Li Xiaoxi , Wan You , Ji Xinqiang , Wang Wei , Kang Xiaozheng , Yan Wanpu , Fan Zhiyi 
Published: February 2020

BackgroundMultimodal opioid‐sparing analgesia is a key component of an enhanced recovery pathway after surgery that aims to improve postoperative recovery. Transcutaneous electrical acupoint stimulation (TEAS) is assumed to alleviate pain and anxiety and to modify the autonomic nervous system. This study aimed to determine the efficacy of TEAS for sedation and postoperative analgesia in lung cancer patients undergoing thoracoscopic pulmonary resection.MethodsA total of 80 patients were randomized into two groups: the TEAS group and the sham TEAS combined with general anesthesia group. Postoperative pain levels at six, 24, 48 hours, and one month after surgery were measured using the visual analogue scale (VAS). Bispectral index (BIS) score during the TEAS prior to anesthetic induction, Observer's Assessment of Alertness/Sedation (OAAS) score, sufentanil consumption during postoperative patient‐controlled intravenous analgesia (PCIA), number of total and effective attempts of PCIA pump use, and incidence of postoperative nausea and vomiting were recorded and analyzed statistically.ResultsPatients in the TEAS group had significantly lower VAS scores at six, 24, and 48 hours after surgery (P < 0.01); lower BIS scores at 10, 20, and 30 minutes before induction (P < 0.01); lower levels of postoperative sufentanil consumption; lower number of PCIA attempts and effective rates (P < 0.01); lower incidences of nausea at 0, six, 24, and 48 hours; and lower incidence of vomiting at 24 hours after surgery (P < 0.05). The postoperative OAAS scores were similar between the groups.ConclusionsTEAS could be a feasible approach for sedation and postoperative analgesia in thoracoscopic pulmonary resection.