bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recent Developments in the Electrochemical Determination of Sulfonamides
Authors: Fu Li , Zhang Xinyi , Ding Su , Chen Fei , Lv Yanfei , Zhang Huaiwei , Zhao Shichao 
Published: October 2020

Background:Sulfonamides are the anti-bacterial and anti-inflammatory drugs synthesized, which are widely used as medical and veterinary antibiotics. However, the excess dosage of sulfonamides can harm human health. Drug residues in animal products also can harm human health through the food chain. The long-term consumption of animal food containing drug residues will cause some toxicity and side effects on human body functions, which will seriously threaten human health.Methods:Electroanalytical methods are attracting much attention because of their advantage over conventional methods, as they are quick, low-cost, high sensitivity, and portable. This review examines the progress made in the selective electrochemical determination of sulfonamides in the last 20 years.Results:In this review, we describe the development of electrochemical methods for sulfonamides determination. Then, we pay special attention to the detection of sulfonamides using molecular imprinting technology. The linear detection range with the limit of detection has been listed for comparison.Conclusion:Electrochemical determination of sulfonamides is a fast, simple, sensitive, and cost-effective approach. The surface modification of commercial electrodes can significantly improve the sensing performance.
Synergistic Combination of Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid and Natural Flavonoid Curcumin Exhibits Anticancer and Antibacterial Activity
Authors: Altundağ Ergül M. , Toprak Kübra , Şanlıtürk Gizem , Güran Mümtaz , Özbilenler Cahit , Kerküklü Namık R. , Yılmaz Ayşe M. , Yalçın Ahmet S. 
Published: October 2020

Background and Objective:
Curcumin is an effective anti-cancer agent used in thyroid cancer treatments.
However, its use in clinical applications is limited due to low solubility and bioavailability. In this study,
a novel combination strategy was applied by combining curcumin with Suberoylanilide Hydroxamic Acid
(SAHA) to increase both bioavailability of curcumin and the efficiency of SAHA, which have limited efficiency
when used alone.

Methods:
MTT assay was used to determine the cell viability of B-CPAP cells upon treatment with SAHA,
curcumin and their combinations. Synergistic interactions between two agents were analyzed by Calcusyn software.
Apoptosis and cell cycle assays were measured by flow cytometry. Expressions of apoptotic and cell cyclerelated
proteins (PARP, P21/CDKN1A/WAF1, P27/KIP1) were examined by western blot analysis. Broth microdilution
assay was performed to determine Minimum Inhibitory Concentration (MIC) values against S. aureus.

Results:
Based on MTT assay, IC50 values for SAHA and curcumin were determined as 0.91μM and 20.97μM,
respectively. The combination index CI value was determined as 0.891 in B-CPAP cells, which demonstrate
synergistic activity. The apoptotic effect was achieved by combination treatment (51.85%) on B-CPAP cells
using half of the dose required for SAHA and curcumin alone. Combination treatment showed a significant
increase in the percentage of B-CPAP cells in the S-phase due to cell arrest. Cleaved-PARP, P21/CDKN1A/
WAF1 and P27/KIP1 protein expressions were upregulated. Curcumin was found to have better anti-microbial
activity than SAHA as having a lower MIC value, and checkerboard synergy analysis revealed that the two
compounds co-operate synergistically for the in vitro killing of S. aureus.

Conclusion:
In the present study, synergistic combinations of SAHA and curcumin were shown to have both
anti-cancer and antibacterial activities that would provide a novel thyroid cancer treatment strategy.
Characteristics of Self-Defining Memories in Middle-Aged and Older Adults
Authors: Cuervo-Lombard Christine , Raucher-Chéné Delphine , Linden Martial Van der , Voltzenlogel Virginie 
Published: October 2020

Background:
Self-Defining Memories (SDMs) are a specific type of autobiographical
memory, which play a key role in the construction of personal identity.

Objective:
We investigated the characteristics of SDMs in elderly subjects. The originality of the
present study is to compare our elderly group to middle-aged subjects instead of young adults, as
previously reported in the literature, to understand the age-related modifications in SDMs.

Method:
We recruited 41 elderly subjects with normal cognitive functioning and 37 middle-aged
adults. They were matched for education level and verbal knowledge.

Result:
Older participants recalled the same number of specific memories than middle-aged participants.
SDMs were predominantly constituted of episodic characteristics, with specific details, in
both the groups. However, middle-aged subjects gave more integrative meaning of SDMs and
more redemptive events than older participants. The two samples differed in three content dimensions
(exploration/recreation, relationship contents, and not classifiable). As predicted, older participants
reported memories that were more positive, on average, than the middle-aged participants’
memories.

Conclusion:
Our study added some contributions to the understanding of the consequences of aging
on the sense of self. Future research should explore the continuity of SDMs characteristics
across the lifespan.
Validation of Texas Christian University Psychosocial Functioning and Motivation Scales in Iranian Persons Who Use Drugs
Authors: Shahrabadi Sana , Jalali Amir , Jalali Rostam , Salari Nader 
Published: October 2020

Background:
The psychosocial functioning and motivation scales of the Texas Cristian
University (TCU) were translated and validated for the Persons Who Use Drugs (PWUDs).

Methods:
This study was carried out as a methodological research. The scales were translated forward
and backward and then, the PWUDs in Kermanshah-based (west of Iran) substance abuse
treatment centers in 2019 filled out the scales. Participants included 250 individuals who were selected
by convenience sampling method. A demographics form and psychosocial functioning and
motivation scales were used to collect data. For data analysis, content validity, face validity, testretest,
internal consistency, and construct validity were applied by using exploratory analysis methods
in SPSS (v.20) and confirmatory factor analysis in LISREL software.

Results:
Exploratory Factor Analysis (EFA) results were obtained by using the main component
analysis and Varimax rotation of three factors (psychological function, social function, treatment
motivation). Extracted factors, along with the eigenvalues, attributed variance percentage, and cumulative
attributed variances by each factor, by 68.56%, showed a total variance of the questionnaire.
Moreover, the fitness indexes of the Confirmatory Factor Analysis (CFA) of psychological
function, social function, and treatment motivation were 0.059, 0.075 and 0.072, respectively.
Therefore, the questionnaire fitness was at an acceptable level. The test-retest reliability coefficient
was 0.875, and the Cronbach's alpha coefficient for all the components was higher than 0.71.

Conclusion:
The results showed that psychosocial functioning, motivation scales have good psychometric
properties, and they can be used in research and treatment fields.
Free Vibration of Functionally Graded Carbon Nanotube-reinforced Doubly-curved Shells
Authors: Janghorban Maziar , Karami Behrouz 
Published: October 2020

Background::
Carbon nanotubes (CNTs) reinforced structures are the main elements of
structural equipment. Hence a wide range of investigations has been performed on the response of
these structures. A lot of studies covered the static and dynamic phenomenon of CNTs reinforced
beams, plates and shells. However, there is no study on the free vibration analysis of a doubly-curved
nano-size shell made of CNTs reinforced composite materials.

Methods::
This work utilized a general third-order shear deformation theory to model the nanoshell
where the general strain gradient theory is used in order to capture both nonlocality and strain gradient
size-dependency. The Navier solution solving procedure is adopted to solve the partial differential
equations (PDEs) and get the natural frequency of the system which is obtained through the
Hamilton principle.

Results::
The current study shows the importance of small-scale coefficients. The natural frequency
increases with rising the strain gradient-size dependency which is because of stiffness enhancement,
while the natural frequency decreases by increasing the nonlocality. In addition, the numerical examples
covered the CNTs distribution patterns.

Conclusion::
This work also studied the importance of shell panel’s shape. It has been observed that
spherical shell panel has a higher frequency compared to the hyperbolic one. Furthermore, the frequency
of the system increases with growing length-to-thickness ration.
The Causes of Lack of Pharmaceutical Logistics Monitoring in Surgery Rooms: A Case Study in a Type C Hospital
Authors: Iswari Luh Putu Ayu Pradnya , Youandi Abdi Agus , Indriani Wiwin 
Published: October 2020

The surgery rooms have an important role in the hospital, so it must be managed properly. The surgery rooms consume 9% of the annual hospital budget. High operational costs caused by the large cost of human resources, medical materials, and other supporting facilities. This study was conducted in a private hospital type C with 181 beds and has four surgery rooms. The number of patients which was operated in X Hospital was very high, and the highest number in July 2019 reached 515 patients. There were a large supply of drugs and medical devices in the surgery rooms.Â The number of each drug reached hundreds ampoule, and medical devices reached tens and some in the hundreds. The management of pharmaceutical supplies in the surgery rooms was carried out by senior nurse head of surgery rooms. Monitoring logistic by pharmacy officer only done once a month. This made excessive of stock and become expired. The purpose of this study was to analyze the factors that cause the lack of pharmaceutical logistics monitoring in the surgery rooms. Determination the root of the problem was done by using a fishbone diagram through a Focus Group Discussion with the division heads and subdivision heads of X Hospital. Focus Group Discussion is done by asking the participants one by one to find the root of the problem based on five factors (man, material, method, machine, and environment). Obtained 8 root problems then analyzed with 5 why to get the main root problem. Furthermore, determine the biggest problem using the CARL (capability, accessibility, readiness, and leverage) method, with a weighting score between 1-5. The results found that the absence of pharmaceutical logistics monitoring SOP in the surgery rooms was the most contributing factor to the lack of pharmaceutical logistics monitoring in the surgery rooms.
ДЕЯКІ ПИТАННЯ ХІРУРГІЧНОЇ ТАКТИКИ В ЛІКУВАННІ ПІГМЕНТНИХ НЕВУСІВ У ДІТЕЙ
Authors: Конопліцький В. С. , Пасічник О. В. , Калінчук О. О. , Конопліцький Д. В. , Блажко С. С. 
Published: October 2020

Резюме. Стаття містить актуальну інформацію щодо діагностики та лікування дітей з меланоцитарними невусами, форми яких зустрічаються найчастіше, з вказівкою на їх динамічну еволюцію з часом. Обговорюються фактори ризику та питання ранньої діагностики меланоми в загальній практиці. Також обговоренню підлягає сучасне розуміння ризиків, пов’язаних з вродженими меланоцитарними невусами різних розмірів, і стратегії лікування дітей з вродженими і набутими меланоцитарними невусами.
Метою дослідження було підвищення якості лікування дітей шляхом уточнення показів і методів хірургічного лікування пігментних невусів шкіри.
Пігментні невуси відносяться до доброякісних утворень, виникнення яких на сучасному етапі пов’язують з міграцією в ембріональному періоді меланобластів із нейроектодермальної трубки в базальний шар епідермісу. У дітей пігментні невуси зустрічаються досить часто в будь-якому віці. Так у новонароджених пігментні невуси зустрічаються в 1–6% загальної популяції. Покази до профілактичного видалення невусів витікають із проведеного аналізу клінічного матеріалу: наявність симптомів активації, зокрема ріст невуса; наявності в анамнезі факторів, що сприяють активації пігментних невусів – підвищена сонячна інсоляція, травматизація, зміна гормонального фону; наявність ознак клітинної проліферації; наявність вогнища термонагріву в проекції пігментного невуса. Обов’язковою умовою виконання операції повинно бути проведення морфологічного дослідження. Об’єм тканин, що видаляються, залежить від форми росту, розмірів невуса.
Íñigo Abbad y Lasierra, Diario del viaje a América, ed. de Juan J. Nieto y José M. Sánchez, Madrid, Miraguano Ediciones, 2003, 334 pp. (Viajes y costumbres)
Authors: Escandón Patricia 
Published: October 2020

Reseña del libro: Íñigo Abbad y Lasierra, Diario del viaje a América, ed.de Juan J. Nieto y José M. Sánchez, Madrid, MiraguanoEdiciones, 2003, 334 pp. (Viajes y costumbres).
Diversity of Arbuscular Mycorrhizal Fungi in Shola Forests of Kodaikanal, Southern India
Authors: Kullaiyan Sathiyadash  , Jaffer Ali Jabeen  , Karthigai Selvi Ezhilarasi  , Eswaran Pillai Uma  
Published: October 2020

Arbuscular mycorrhizal fungi establish symbiotic association with more than 80% of land plants and influence plant community composition and distribution. The mycorrhizal status of plant species in various ecosystems have been reported but failed to assess community composition of AM fungi in shola forests, therefore present study carried out to fulfill this research gap. A total of Seven AM fungal species could be isolated from Tiger and Kookal shoal and identified based on spore morphology. There is no significant difference among distribution of AM fungal spores in various seasons among sites. In both the sholas AM fungal spore numbers were high during the wet (September) season and the variation among spore numbers in different seasons among sites were insignificant. Arbuscular mycorrhizal spore of Funneliformis geosporum was the most frequent species and Funneliformis mosseae was the less frequent species. The frequency among various sites in both the Sholas was insignificant. The AM fungal species richness was high in Tiger Shola (six spores), whereas in Kookal Shola showed less species richness. The diversity indices like Shanon - Weaver index (H') and Simpson index (D) were calculated in all the sites and showed variation among shoals in four sites. Arbuscular mycorrhizal fungal community composition in this ecosystem have been elucidated to certain extent and used for conservation of shola species.
Body Mass Index of selected Overweight and Obese Indian Adolescents in Madurai district, Tamil Nadu, India
Authors: B. Bhuvaneswari  , S. Parameshwari  
Published: October 2020

Childhood obesity is an important predisposing factor for most non-communicable diseases. Irrespective of the age and gender, the prevalence rates of overweight and obesity has attained alarming levels not only in developed countries but also in developing countries among all socio-economic groups. The prevalence of overweight and obesity has reached alarming levels, affecting virtually both developed and developing countries of all socio-economic groups, irrespective of their age and gender. Hence, the present study was aimed to identify the anthropometric measurements of selected overweight and obese school-going adolescents from different schools from Madurai district Tamil Nadu, India. A total of 514 adolescents (312 Girls and 202 Boys) aged 13-18 years participated from government and private schools. Mean height, weight and BMI were calculated and compared with (National centre for Health Statistics) NCHS standard references. The mean height, weight and BMI were found to be increasing as the age increases of both genders. Body Mass Index of Girls (34.18 – 35.24)was found to be higher than boys (33.49 – 32.89) of the same age group. The School-going adolescents of both genders, in general, have short stature at all ages as compared to NCHS reference data. The prevalence of Overweight 95 (18.48%) and obese 315 (61.28%) was found to be higher in Nuclear families, but there was no statistical association between family type and BMI (X2 = 2.575, p > .05). A significant relationship was found between the Body Mass Index of the adolescents and Father’s Educational qualification (p=.00) and Mother’s Occupation (p=.008). Nutrition education can play an important part in reducing the incidence of overweight/obesity and its associated complications.Extensive trials with a healthy school environment as an intervention may pave the way for developing new strategies to reduce the overweight and obesity in school-going adolescents.
A Comparative Study on Toxicity of Recombinant Chitinase on A Polyphagous Pest, Helicoverpa Armigera
Authors: Esther Shoba R  
Published: October 2020

Holometabolous insects complete their life cycle in four stages: egg, larva, pupa and adult. The larval stage is characterized by instar and on an average most larvae has 4- 6 instar stages that occurs between molts. One of the essential enzyme for the process of moulting is chitinase. Expression of chitinase gene takes place when the insect is ready to moult, wherein the old exoskeleton being made of chitin is replaced with the new exoskeleton. Reports indicate that the introduction of the chitinase enzyme at inappropriate time and at inappropriate concentration will prevent the formation of chitin, resulting in no moult, thus growth retardation of the insect and finally death. Insects own chitinase has shown to be a potential insecticide. In the present study, gene encoding for Helicoverpa armigera chitinase was isolated and the sequence was found to be of 1734bp as compared to the reference sequence. The gene was expressed in a prokaryotic and eukaryotic host system. The protein was purified using Ni- NTA column as the protein was tagged with histidine. Chitinase activity was determined and the obtained enzyme was used for toxicity studies against the same insect. Injection assay, topical application and oral ingestion studies showed that Pichia pastoris expressed recombinant chitinase was more effective as compared to the Escherichia coli expressed recombinant chitinase. In case of injection assay, P. pastoris expressed recombinant chitinase recorded 77% mortality for the highest concentration used. Topical application showed 58% and 40% of malformed pupae for Pichia pastoris end E. coli expressed recombinant chitinase. An significant decrease in weight gain was observed on 2nd day for P. pastoris expressed recombinant chitinase and from 7th day for E.coli expressed recombinant chitinase in the oral ingestion studies. Effectiveness of Pichia pastoris expressed recombinant chitinase might be because of post translational modifications in the eukaryotic system.
Phytochemical Screening, GC-MS & HPLC Analysis of Decalepis arayalpathra (Joseph & Chandras) an Endangered Medicinal Plant
Authors: Ashalatha K S  , Raveesha H R  
Published: October 2020

Decalepis arayalpathra is one of the important medicinal plants with milky latex, which grows on the exposed rocky slopes at an
800-1200 m elevation. It is endemic to the southern region of the Western Ghats chiefly in the Agasthyamalai region of Tamil Nadu. The plant
has been used by traditional people to treat various diseases such as peptic ulcers, cancer like affliction, skin diseases etc. The moniliform tuberous roots have special fragrance like vanilla, because the roots contain different compounds such as α-amyrin acetate, ß-sitosterol, naringenin, kaempferol and aromadendrin especially in 2-hydroxy 4-methoxy benzaldehyde (2H4MB). The present study was aimed to examine the phytochemical screening and identification of bioactive compounds of Decalepis arayalpathra by GC-MS & HPLC analysis. The phytochemical analysis was carried out by using standard methods. Preliminary phytochemical screening showed major bioactive compounds such as alkaloids, flavonoids, proteins, steroids, terpenoids, quinones, resins, phenols, tannins, volatile oils and other phytocompounds. FTIR analysis identified various bioactive components and those were confirmed through GC-MS analysis. 2-hydroxy 4-methoxybenzaldehyde (2H4MB) and ß-sitosterol were quantified by HPLC analysis. Further studies have to be carried out in the in-vitro multiplication, isolation and characterization of compounds to enhance the root flavouring complexes for medicinal, food and beverage industries.
Isolation and Molecular Profiling Of Thermoresistant Bacteria From Chimneys Of Baking Industries Of Madurai, Tamilnadu, India
Authors: V.Parthasarathy  , R. Babu Rajendran  
Published: October 2020

The present study was chosen to discover the thermophiles isolation from chimneys of baking industries o Madurai, Tamilnadu, India. An attempt has been made to unearth the conserved sequences among them. Phylogenetic neighbour joining tree based on their 16S rRNA showing a relationship with related organisms has been constructed for each isolates keeping a species as an outgroup. In Madurai, baking industries have been found to be one of the hottest area having temperature as high as 120°C. Incubations of the bacterial colonies were done with covered water bath incubator at 70°C for 72-96 hrs in Castenholz TYE broth. The temperature range of 40-100°C, pH range of 4.0-10.0 and incubation period of 24-144 hrs was investigated for the best growth of thermophilic bacteria. Samples were collected in sterilized screw capped vials from different sites of Madurai Baking Industries and brought to the laboratory and kept at 4°C in the refrigerator till further processing. PCR products were sequenced using same primers by a commercial sequencing facility (Xcleris lab). The sequences of these ten bacterial isolates after sequencing were blasted using online NCBI BLAST program. Ten thermotolerant bacteria isolates were isolated and the optimal temperature for growth of the isolates was 70°C and the optimal pH was 7.0. 16S rRNA gene sequences of all these thermophilic bacterial isolates exhibited 98-99% homology with Bacillus sp. The phylogenetic analysis of the 16S rRNA gene sequences also confirmed the relationship of these thermophilic isolates with the genus Bacillus The 16S rRNA gene of all isolates was amplified, sequenced, and insilico analysis of these ten sequences was carried out. Based on BLAST alignment of 16S rRNA gene sequences of these isolates to GenBank sequences, these all isolates belonged to genus Bacillus and showed 98-99% similarity. The present study delves in to isolation, identification, characterisation,and analysis o the genomes of bacterial isolates such as Bacillus sp. VP1, Bacillus sp. VP2, Bacillus sp. VP3,Pseudomonas sp. VP4, Pseudomonas sp VP5,Bacillus licheniformis strain VP7,Bacillus sp. VP8, Bacillus sp. VP9, Bacillus altitudinis sp. VP10, and Bacillus cereus strain VP11.