bluuebunny/crossref_metadata_2025_split huggingface dataset:
The short life of the volcanic island New Late’iki (Tonga) analyzed by multi-sensor remote sensing data
Authors: Plank Simon , Marchese Francesco , Genzano Nicola , Nolde Michael , Martinis Sandro 
Published: December 2020

AbstractSatellite-based Earth observation plays a key role for monitoring volcanoes, especially those which are located in remote areas and which very often are not observed by a terrestrial monitoring network. In our study we jointly analyzed data from thermal (Moderate Resolution Imaging Spectrometer MODIS and Visible Infrared Imaging Radiometer Suite VIIRS), optical (Operational Land Imager and Multispectral Instrument) and synthetic aperture radar (SAR) (Sentinel-1 and TerraSAR-X) satellite sensors to investigate the mid-October 2019 surtseyan eruption at Late’iki Volcano, located on the Tonga Volcanic Arc. During the eruption, the remains of an older volcanic island formed in 1995 collapsed and a new volcanic island, called New Late’iki was formed. After the 12 days long lasting eruption, we observed a rapid change of the island’s shape and size, and an erosion of this newly formed volcanic island, which was reclaimed by the ocean two months after the eruption ceased. This fast erosion of New Late’iki Island is in strong contrast to the over 25 years long survival of the volcanic island formed in 1995.
The RabGEF ALS2 is a hypoxia inducible target associated with the acquisition of aggressive traits in tumor cells
Authors: Rivas Solange , Silva Patricio , Reyes Montserrat , Sepúlveda Hugo , Solano Luis , Acuña Juan , Guerrero Marisol , Varas-Godoy Manuel , Quest Andrew F. G. , Montecino Martín , Torres Vicente A. 
Published: December 2020

AbstractTumor hypoxia and the hypoxia inducible factor-1, HIF-1, play critical roles in cancer progression and metastasis. We previously showed that hypoxia activates the endosomal GTPase Rab5, leading to tumor cell migration and invasion, and that these events do not involve changes in Rab protein expression, suggesting the participation of intermediate activators. Here, we identified ALS2, a guanine nucleotide exchange factor that is upregulated in cancer, as responsible for increased Rab5-GTP loading, cell migration and metastasis in hypoxia. Specifically, hypoxia augmented ALS2 mRNA and protein levels, and these events involved HIF-1α-dependent transcription, as shown by RNAi, pharmacological inhibition, chromatin immunoprecipitation and bioinformatics analyses, which identified a functional HIF-1α-binding site in the proximal promoter region of ALS2. Moreover, ALS2 and Rab5 activity were elevated both in a model of endogenous HIF-1α stabilization (renal cell carcinoma) and by following expression of stable non-hydroxylatable HIF-1α. Strikingly, ALS2 upregulation in hypoxia was required for Rab5 activation, tumor cell migration and invasion, as well as experimental metastasis in C57BL/6 mice. Finally, immunohistochemical analyses in patient biopsies with renal cell carcinoma showed that elevated HIF-1α correlates with increased ALS2 expression. Hence, this study identifies ALS2 as a novel hypoxia-inducible gene associated with tumor progression and metastasis.
Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma
Authors: Kågedal Åsa , Hjalmarsson Eric , Farrajota Neves da Silva Pedro , Piersiala Krzysztof , Georén Susanna Kumlien , Margolin Gregori , Munck-Wikland Eva , Winqvist Ola , Häyry Valtteri , Cardell Lars Olaf 
Published: December 2020

AbstractRecurrence in oral squamous cell carcinoma (OSCC) significantly reduces overall survival. Improved understanding of the host’s immune status in head and neck cancer may facilitate identification of patients at higher risk of recurrence and improve patients’ selection for ongoing clinical trials assessing the effectiveness of immune checkpoint inhibitors (CPI). We aimed to investigate Sentinel Node-derived T-cells and their impact on survival. We enrolled prospectively 28 OSCC patients treated at Karolinska University Hospital, Stockholm, Sweden with primary tumour excision and elective neck dissection. On top of the standard treatment, the enrolled patients underwent sentinel node procedure. T cells derived from Sentinel nodes, non-sentinel nodes, primary tumour and PBMC were analyzed in flow cytometry. Patients who developed recurrence proved to have significantly lower level of CD4+ CD69+ in their sentinel node (31.38 ± 6.019% vs. 43.44 ± 15.33%, p = 0.0103) and significantly higher level of CD8+ CD HLA-DR+ (38.95 ± 9.479% vs. 24.58 ± 11.36%, p = 0.0116) compared to disease-free individuals. Survival analysis of studied population revealed that patients with low proportion of CD4+ CD69+ had significantly decreased disease-free survival (DFS) of 19.7 months (95% CI 12.6–26.9) compared with 42.6 months (95% CI 40.1–45.1) in those with high CD4+ CD69+ proportion in their Sentinel Nodes (log-rank test, p = 0.033). Our findings demonstrate that characterization of T-cell activation in Sentinel Node serves as a complementary prognostic marker. Flow cytometry of Sentinel Node may be useful in both patients’ surveillance and selection for ongoing CPI clinical trials in head and neck cancer.
Central dopamine D2 receptors regulate plasma glucose levels in mice through autonomic nerves
Authors: Ikeda Hiroko , Yonemochi Naomi , Mikami Risa , Abe Manabu , Kawamura Meiko , Natsume Rie , Sakimura Kenji , Waddington John L. , Kamei Junzo 
Published: December 2020

AbstractRecent evidence suggests that the central nervous system (CNS) regulates plasma glucose levels, but the underlying mechanism is unclear. The present study investigated the role of dopaminergic function in the CNS in regulation of plasma glucose levels in mice. I.c.v. injection of neither the dopamine D1 receptor agonist SKF 38393 nor the antagonist SCH 23390 influenced plasma glucose levels. In contrast, i.c.v. injection of both the dopamine D2 receptor agonist quinpirole and the antagonist l-sulpiride increased plasma glucose levels. Hyperglycemia induced by quinpirole and l-sulpiride was absent in dopamine D2 receptor knockout mice. I.c.v. injection of quinpirole and l-sulpiride each increased mRNA levels of hepatic glucose-6-phosphatase and phosphoenolpyruvate carboxykinase, which are the key enzymes for hepatic gluconeogenesis. Systemic injection of the β2 adrenoceptor antagonist ICI 118,551 inhibited hyperglycemia induced by l-sulpiride, but not by quinpirole. In contrast, hyperglycemia induced by quinpirole, but not by l-sulpiride, was inhibited by hepatic vagotomy. These results suggest that stimulation of central dopamine D2 receptors increases plasma glucose level by increasing hepatic glucose production through parasympathetic nerves, whereas inhibition of central dopamine D2 receptors increases plasma glucose level by increasing hepatic glucose production through sympathetic nerves.
Screening of native microalgae species for carbon fixation at the vicinity of Malaysian coal-fired power plant
Authors: Yahya Liyana , Harun Razif , Abdullah Luqman Chuah 
Published: December 2020

AbstractGlobal warming has become a serious issue nowadays as the trend of CO2 emission is increasing by years. In Malaysia, the electricity and energy sector contributed a significant amount to the nation’s CO2 emission due to fossil fuel use. Many research works have been carried out to mitigate this issue, including carbon capture and utilization (CCUS) technology and biological carbon fixation by microalgae. This study makes a preliminary effort to screen native microalgae species in the Malaysian coal-fired power plant’s surrounding towards carbon fixation ability. Three dominant species, including Nannochloropsis sp., Tetraselmis sp., and Isochrysis sp. were identified and tested in the laboratory under ambient and pure CO2 condition to assess their growth and CO2 fixation ability. The results indicate Isochrysis sp. as the superior carbon fixer against other species. In continuation, the optimization study using Response Surface Methodology (RSM) was carried out to optimize the operating conditions of Isochrysis sp. using a customized lab-scale photobioreactor under simulated flue gas exposure. This species was further acclimatized and tested under actual flue gas generated by the power plant. Isochrysis sp. had shown its capability as a carbon fixer with CO2 fixation rate of 0.35 gCO2/L day under actual coal-fired flue gas exposure after cycles of acclimatization phase. This work is the first to demonstrate indigenous microalgae species' ability as a carbon fixer under Malaysian coal-fired flue gas exposure. Thus, the findings shall be useful in exploring the microalgae potential as a biological agent for carbon emission mitigation from power plants more sustainably.
Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans
Authors: Tani Shuji , Nishio Naotaka , Kai Kenji , Hagiwara Daisuke , Ogata Yoshiyuki , Tojo Motoaki , Sumitani Jun-ichi , Judelson Howard S. , Kawaguchi Takashi 
Published: December 2020

AbstractTo characterize the molecular mechanisms underlying life-stage transitions in Phytophthora infestans, we initiated a chemical genetics approach by screening for a stage-specific inhibitor of morphological development from microbial culture extracts prepared mostly from actinomycetes from soil in Japan. Of the more than 700 extracts, one consistently inhibited Ph. infestans cyst germination. Purification and identification of the active compound by ESI–MS, 1H-NMR, and 13C-NMR identified β-rubromycin as the inhibitor of cyst germination (IC50 = 19.8 μg/L); β-rubromycin did not inhibit growth on rye media, sporangium formation, zoospore release, cyst formation, or appressorium formation in Ph. infestans. Further analyses revealed that β-rubromycin inhibited the germination of cysts and oospores in Pythium aphanidermatum. A chemical genetic approach revealed that β-rubromycin stimulated the expression of RIO kinase-like gene (PITG_04584) by 60-fold in Ph. infestans. Genetic analyses revealed that PITG_04584, which lacks close non-oomycete relatives, was involved in zoosporogenesis, cyst germination, and appressorium formation in Ph. infestans. These data imply that further functional analyses of PITG_04584 may contribute to new methods to suppress diseases caused by oomycetes.
Self-assembled particulate vaccine elicits strong immune responses and reduces Mycobacterium avium subsp. paratuberculosis infection in mice
Authors: Gupta Sandeep K. , Parlane Natalie A. , Luo Dongwen , Rehm Bernd H. A. , Heiser Axel , Buddle Bryce M. , Wedlock D. Neil 
Published: December 2020

AbstractMycobacterium aviumsubspeciesparatuberculosis(MAP) causes chronic progressive granulomatous enteritis leading to diarrhoea, weight loss, and eventual death in ruminants. Commercially available vaccines provide only partial protection against MAP infection and can compromise the use of bovine tuberculosis diagnostic tests. Here, we report the development of a protein-particle-based vaccine containing MAP antigens Ag85A202–347-SOD1–72-Ag85B173–330-74F1–148+669–786as a fusion (‘MAP fusion protein particle’). The fusion antigen displayed on protein particles was identified using mass spectrometry. Surface exposure and accessibility of the fusion antigen was confirmed by flow cytometry and ELISA. The MAP fusion protein particle vaccine induced strong antigen-specific T-cell immune responses in mice, as indicated by increased cytokine (IFN-γ and IL-17A) and costimulatory signals (CD40 and CD86) in these animals. Following MAP-challenge, a significant reduction in bacterial burden was observed in multiple organs of the mice vaccinated with the MAP fusion protein particle vaccine compared with the PBS group. The reduction in severity of MAP infection conferred by the MAP fusion protein particle vaccine was similar to that of Silirum and recombinant protein vaccines. Overall, the results provide evidence that MAP antigens can be engineered as a protein particulate vaccine capable of inducing immunity against MAP infection. This utility offers an attractive platform for production of low-cost particulate vaccines against other intracellular pathogens.
Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution
Authors: Oguchi Yusuke , Shintaku Hirofumi , Uemura Sotaro 
Published: December 2020

AbstractSingle-cell transcriptome analysis has been revolutionized by DNA barcodes that index cDNA libraries, allowing highly multiplexed analyses to be performed. Furthermore, DNA barcodes are being leveraged for spatial transcriptomes. Although spatial resolution relies on methods used to decode DNA barcodes, achieving single-molecule decoding remains a challenge. Here, we developed an in-house sequencing system inspired by a single-molecule sequencing system, HeliScope, to spatially decode DNA barcode molecules at single-molecule resolution. We benchmarked our system with 30 types of DNA barcode molecules and obtained an average read length of ~20 nt with an error rate of less than 5% per nucleotide, which was sufficient to spatially identify them. Additionally, we spatially identified DNA barcode molecules bound to antibodies at single-molecule resolution. Leveraging this, we devised a method, termed “molecular foot printing”, showing potential for applying our system not only to spatial transcriptomics, but also to spatial proteomics.
β-arrestin mediates communication between plasma membrane and intracellular GPCRs to regulate signaling
Authors: DeNies Maxwell S. , Smrcka Alan V. , Schnell Santiago , Liu Allen P. 
Published: December 2020

AbstractIt has become increasingly apparent that G protein-coupled receptor (GPCR) localization is a master regulator of cell signaling. However, the molecular mechanisms involved in this process are not well understood. To date, observations of intracellular GPCR activation can be organized into two categories: a dependence on OCT3 cationic channel-permeable ligands or the necessity of endocytic trafficking. Using CXC chemokine receptor 4 (CXCR4) as a model, we identified a third mechanism of intracellular GPCR signaling. We show that independent of membrane permeable ligands and endocytosis, upon stimulation, plasma membrane and internal pools of CXCR4 are post-translationally modified and collectively regulate EGR1 transcription. We found that β-arrestin-1 (arrestin 2) is necessary to mediate communication between plasma membrane and internal pools of CXCR4. Notably, these observations may explain that while CXCR4 overexpression is highly correlated with cancer metastasis and mortality, plasma membrane localization is not. Together these data support a model where a small initial pool of plasma membrane-localized GPCRs are capable of activating internal receptor-dependent signaling events.
Submolecular probing of the complement C5a receptor–ligand binding reveals a cooperative two-site binding mechanism
Authors: Dumitru Andra C. , Deepak R. N. V. Krishna , Liu Heng , Koehler Melanie , Zhang Cheng , Fan Hao , Alsteens David 
Published: December 2020

AbstractA current challenge to produce effective therapeutics is to accurately determine the location of the ligand-biding site and to characterize its properties. So far, the mechanisms underlying the functional activation of cell surface receptors by ligands with a complex binding mechanism remain poorly understood due to a lack of suitable nanoscopic methods to study them in their native environment. Here, we elucidated the ligand-binding mechanism of the human G protein-coupled C5a receptor (C5aR). We discovered for the first time a cooperativity between the two orthosteric binding sites. We found that the N-terminus C5aR serves as a kinetic trap, while the transmembrane domain acts as the functional site and both contributes to the overall high-affinity interaction. In particular, Asp282 plays a key role in ligand binding thermodynamics, as revealed by atomic force microscopy and steered molecular dynamics simulation. Our findings provide a new structural basis for the functional and mechanistic understanding of the GPCR family that binds large macromolecular ligands.
Surface-wave instability without inertia in shear-thickening suspensions
Authors: Darbois Texier Baptiste , Lhuissier Henri , Forterre Yoël , Metzger Bloen 
Published: December 2020

AbstractRecent simulations and experiments have shown that shear-thickening of dense particle suspensions corresponds to a frictional transition. Based on this understanding, non-monotonic rheological laws have been proposed and successfully tested in rheometers. These recent advances offer a unique opportunity for moving beyond rheometry and tackling quantitatively hydrodynamic flows of shear-thickening suspensions. Here, we investigate the flow of a shear-thickening suspension down an inclined plane and show that, at large volume fractions, surface kinematic waves can spontaneously emerge. Curiously, the instability develops at low Reynolds numbers, and therefore does not fit into the classical framework of Kapitza or ‘roll-waves’ instabilities based on inertia. We show that this instability, that we call ‘Oobleck waves’, arises from the sole coupling between the non-monotonic (S-shape) rheological laws of shear-thickening suspensions and the flow free surface.
Quantum annealing using vacuum states as effective excited states of driven systems
Authors: Goto Hayato , Kanao Taro 
Published: December 2020

AbstractQuantum annealing, which is particularly useful for combinatorial optimization, becomes more powerful by using excited states, in addition to ground states. However, such excited-state quantum annealing is prone to errors due to dissipation. Here we propose excited-state quantum annealing started with the most stable state, i.e., vacuum states. This counterintuitive approach becomes possible by using effective energy eigenstates of driven quantum systems. To demonstrate this concept, we use a network of Kerr-nonlinear parametric oscillators, where we can start excited-state quantum annealing with the vacuum state of the network by appropriately setting initial detuning frequencies for the oscillators. By numerical simulations of four oscillators, we show that the present approach can solve some hard instances whose optimal solutions cannot be obtained by standard ground-state quantum annealing because of energy-gap closing. In this approach, a nonadiabatic transition at an energy-gap closing point is rather utilized. We also show that this approach is robust against errors due to dissipation, as expected, compared to quantum annealing started with physical excited (i.e., nonvacuum) states. These results open new possibilities for quantum computation and driven quantum systems.
Airborne microplastic particles detected in the remote marine atmosphere
Authors: Trainic Miri , Flores J. Michel , Pinkas Iddo , Pedrotti Maria Luiza , Lombard Fabien , Bourdin Guillaume , Gorsky Gabriel , Boss Emmanuel , Rudich Yinon , Vardi Assaf , Koren Ilan 
Published: December 2020

AbstractAnthropogenic pollution from marine microplastic particles is a growing concern, both as a source of toxic compounds, and because they can transport pathogens and other pollutants. Airborne microplastic particles were previously observed over terrestrial and coastal locations, but not in the remote ocean. Here, we collected ambient aerosol samples in the North Atlantic Ocean, including the remote marine atmosphere, during the Tara Pacific expedition in May-June 2016, and chemically characterized them using micro-Raman spectroscopy. We detected a range of airborne microplastics, including polystyrene, polyethylene, polypropylene, and poly-silicone compounds. Polyethylene and polypropylene were also found in seawater, suggesting local production of airborne microplastic particles. Terminal velocity estimations and back trajectory analysis support this conclusion. For technical reasons, only particles larger than 5 µm, at the upper end of a typical marine atmospheric size distribution, were analyzed, suggesting that our analyses underestimate the presence of airborne microplastic particles in the remote marine atmosphere.
Near-complete loss of fire-resistant primary tropical forest cover in Sumatra and Kalimantan
Authors: Nikonovas Tadas , Spessa Allan , Doerr Stefan H. , Clay Gareth D. , Mezbahuddin Symon 
Published: December 2020

AbstractDeforestation in Indonesia in recent decades has made increasingly large parts of the region vulnerable to fires. Burning is particularly widespread in deforested peatlands, and it leads to globally significant carbon emissions. Here we use satellite-based observations to assess loss and fragmentation of primary forests and associated changes in fire regimes in Sumatra and Kalimantan between 2001 and 2019. We find that fires did not penetrate undisturbed primary forest areas deeper than two kilometres from the forest edge irrespective of drought conditions. However, fire-resistant forest now covers only 3% of peatlands and 4.5% of non-peatlands; the majority of the remaining primary forests are severely fragmented or degraded due to proximity to the forest edge. We conclude that protection and regeneration of the remaining blocks of contiguous primary forest, as well as peatland restoration, are urgently needed to mitigate the impacts of potentially more frequent fire events under future global warming.
Magnetism in a helicate complexes arising with the tetradentate ligand
Authors: Ohmagari Hitomi , Nakaya Manabu , Tanaka Kaisei , Zenno Hikaru , Akiyoshi Ryohei , Sekine Yoshihiro , Zhang Yingjie , Min Kil Sik , Hasegawa Miki , Lindoy Leonard F. , Hayami Shinya 
Published: December 2020

The synthesis of [M(dimphen)(NCS)2] (MII = Fe (1), Co (2), Mn (3)) and [Fe(dimphen)(NCSe)2] (4), where dimphen = [1,2-bis(9-methyl-1,10-phenanthrolin-2-yl)ethane], are reported. 1 shows ferromagnetic interaction, and 2 displays single-molecular magnet behaviour.
Linking Peer-Mediated Interventions to Address Conversational Difficulties in Adolescents With Autism
Authors: Bambara Linda M. , Cole Christine L. , Thomas Amanda 
Published: December 2020

PurposeAdolescents with autism spectrum disorder (ASD) commonly experience an array of pragmatic language difficulties that can interfere with their ability to engage in reciprocal or balanced conversations with their peers. Difficulties with the use of language during peer encounters can contribute to these students' social isolation even when in inclusive settings. Increasingly, researchers have identified effective approaches for teaching conversational skills to individuals with ASD, but there are few demonstrations of effective interventions in high school settings. Peer-mediated intervention (PMI) is one approach that can embody research-based recommendations for teaching conversational skills (e.g., direct instruction, teaching in natural settings, use of peer partners). This clinical focus article describes effective PMI strategies for improving the social conversations of high school students with ASD based on our (the authors') emerging research.ConclusionsUsingFey's (1986)classification scheme, the article begins with an illustration of different conversational profiles of adolescents with ASD and associated goals for intervention. Next, key components of our PMI approach are described, followed by detailed illustrations of three research-based intervention strategies that speech-language pathologists can use to address different conversational goals. Emphasis is placed on strategies for teaching students with ASD target skills and training peers to support conversation. Outcomes and clinical reflections of our intervention research are described. Finally, the article ends with a discussion of additional considerations for speech-language pathologists regarding implementation and generalization.Supplemental Materialhttps://doi.org/10.23641/asha.13308470