bluuebunny/crossref_metadata_2025_split huggingface dataset:
Probucol enhances the therapeutic efficiency of mesenchymal stem cells in the treatment of erectile dysfunction in diabetic rats by prolonging their survival time via Nrf2 pathway
Authors: Wang Haoran , Zhang Keqin , Ruan Zheng , Sun Dingqi , Zhang Hui , Lin Guiting , Hu Liangliang , Zhao Shengtian , Fu Qiang 
Published: February 2020

Abstract
Background Intracavernous injection of mesenchymal stem cells (MSCs) is a promising method for Diabetic mellitus-induced erectile dysfunction (DMED), but short survival time of MSCs in cavernous is fatal defect for therapy. This study investigated therapeutic efficiency and potential mechanism of Probucol combined with MSCs. Methods In vivo study, total forty-eight 10-week-old male Sprague-Dawley (SD) rats were used in this study. Twelve rats received intraperitoneal injection of PBS as Sham Group; the rest received intraperitoneal injection of 60mg/kg streptozotocin to establish DM models. DM rats were randomly divided into three groups: received intracavernosal (IC) injection of either PBS (DM Group), MSCs (M Group), and administrated Probucol after intracavernosal injection of MSCs (P+M Group). Erectile function was assessed by electrical stimulation of the cavernous nerves with real-time intracavernous pressure measurement. After euthanasia, penile tissue was investigated for histologic examination and Western blotting. In vitro experiment, H 2 O 2 was used to create oxidative stress environment to detect changes in cell viability. CCK8 was used to measure cell viability of MSCs treated with or without Probucol. Intracellular ROS changes were detected by flow cytometry. Autophagy and apoptosis were detected by Western blotting and confocal microscopy. Results Recovery of erectile function was observed in the P+M Group. The combination therapy decreased fibrosis and increased endothelial function compared with MSCs therapy alone. Western blotting results confirmed the increased expression of Nrf2 and HO-1 in cavernous body. Vitro experiment indicated H 2 O 2 -induced high oxidative stress reduced cell viability, which was gradually reversed with increased concentration of Probucol. H 2 O 2 reduced Nrf2 expression, which was reversed by Probucol's intervention. Furthmore, the expression of Bax, Caspase3 and Cleaved-Caspase3 decreased, and the expression of Bcl-2 increased in a dose-dependent manner because of Probucol's intervention. In addition, Beclin1 and LC3II both increased in a dose-dependent manner. Meanwhile, the expression of P62 decreased. In study of autophagy flux, we found Probucol didn’t block it. Conclusion Probucol enhanced therapeutic efficiency of MSCs in DMED by prolonging their survival time, which mediated through improving the transplanted microenvironment of MSCs, increasing self-antioxidant ability of MSCs, strengthening protective autophagy and inhibiting apoptosis of MSCs via Nrf2 pathway.
Molecular hydrogen induces metabolic reprogramming to promote differentiation of glioma stem cells
Authors: Ma Sheng-nan , Xie Fei , Zhang Yan , Yao Ting-ting , Liu Meng-yu , Zhang Xiao-kang , Zhao Peng-xiang , Ma Xuemei 
Published: February 2020

Abstract
Background: Our previous study showed that molecular hydrogen could effectively suppress glioblastoma multiforme (GBM) tumor growth via induction of glioblastoma stem cells (GSCs) differentiation. Metabolic reprogramming has been demonstrated to delicately regulate the stemness of cancer stem cells. In the present study, we explored whether metabolic reprogramming is involved in the hydrogen-induced GSCs differentiation. Methods: Immunofluorescence staining was conducted to determine the expression of cell surface markers. Glucose uptake and lactate secretion was determined by spectrophotometric method. Untargeted metabolomics analyses were conducted to investigate the metabolic alteration in GSCs after hydrogen treatment. 13 C-labeled metabolic flux analysis was performed to explore the regulatory effects of molecular hydrogen on the glucose metabolism of GSCs. Results: Immunofluorescence staining showed the up-regulated expression of oligodendroglial markers in hydrogen-treated GSCs. Both glucose uptake and lactate production in GSCs were significantly inhibited by hydrogen treatment. Untargeted metabolomics analyses showed hydrogen-induced promotion of de novo synthesis of nucleotides in GSCs. Metabolic flux analysis showed decreased glucose metabolism in GSCs induced by hydrogen. Conversely, the content of glycerol 3-phosphate, glutamate and glutamine were increased by hydrogen treatment. Conclusions: Results from this study demonstrated that molecular hydrogen could inhibit glucose metabolism and promote de novo synthesis of nucleotides in GSCs, suggesting the involvement of metabolic reprogramming in the hydrogen-induced GSCs differentiation. Our study also provides important new clues to seek the target of molecular hydrogen.
Identification and genetic analysis of qCL1.2, a novel allele of the “green revolution” gene SD1 from wild rice (Oryza rufipogon) that enhances plant height
Authors: Zhang Lizhen , Huang Jingfen , Wang Yanyan , Xu Rui , Yang Ziyi , Zhao Zigang , Liu Shijia , Tian Yunlu , Zheng Xiaoming , Li Fei , Wang Junrui , Song Yue , Li Jiaqi , Cui Yongxia , Zhang Lifang , Cheng Yunlian , Lan Jinhao , Qiao Weihua , Yang Qingwen 
Published: February 2020

Abstract
Background: The exploitation of novel alleles from wild rice that were lost during rice cultivation could be very important for rice breeding and evolutionary studies. Plant height (PH) was a target of artificial selection during rice domestication and is still a target of modern breeding. The “green revolution” gene semi-dwarf 1 (SD1) were well documented and used in the past decades, allele from wild rice could provide new insights into the functions and evolution of this gene.Results: We identified a PH-related quantitative trait locus, qCL1.2, from wild rice using a set of chromosome segment substitution lines. qCL1.2 encodes a novel allele of SD1 gene. The wild allele of SD1 is a dominant locus that can significantly promote rice internode length by regulating the expression levels of genes involved in gibberellin biosynthesis and signal transduction. Nucleotide diversity and haplotype network analyses of the SD1 gene were performed using 2,822 rice landraces. Two previously reported functional nucleotide polymorphisms clearly differentiated japonica and indica rice; however, they were not associated with PH selection. Other new functional nucleotide polymorphisms in the coding, but not promoter, regions were involved in PH selection during rice domestication. Our study increases understanding of the rice SD1 gene and provides additional evidence of this gene’s selection during rice domestication.Conclusions: Our findings provide evidence that SD1 gene from wild rice enhances plant height and new functional nucleotide polymorphisms of this gene were artificially selected during cultivated rice differentiation.
The features of polyglutamine regions depend on their evolutionary stability
Authors: Mier Pablo , Andrade-Navarro Miguel A. 
Published: February 2020

Abstract
Background Polyglutamine regions (polyQ) are one of the most studied and prevalent homorepeats in eukaryotes. They have a particular length-dependent codon usage, which relates to a characteristic CAG-slippage mechanism. Pathologically expanded tracts of polyQ are known to form aggregates and are involved in the development of several human neurodegenerative diseases. The non-pathogenic function of polyQ is to mediate protein-protein interactions via a coiled-coil pairing with an interactor. They are usually located in a helical context.Results Here we show how these known features related to polyQ depend on their stability in evolution. We have classified the polyQ regions of 60 proteomes from four distinct taxonomic groups (Insecta, Teleostei, Sauria and Mammalia) in three main categories based on their evolutionary stability. Codon usage, amino acid context, structural conformation and the protein-protein interaction capacity of polyQ from all studied taxa critically depend on the region stability.Conclusions Our results show that apart from the sequence of a polyQ, information about its orthologous sequences is needed to assess its function.
Heme oxygenase-1 deficiency presenting with interstitial lung disease and hemophagocytic flares
Authors: Chau Alice S. , Cole Bonnie L. , Debley Jason S. , Nanda Kabita , Rosen Aaron B.I. , Bamshad Michael J. , Nickerson Deborah A. , Torgerson Troy R. , Allenspach Eric J. 
Published: February 2020

Abstract
Background Heme oxygenase-1 (HMOX1) catalyzes the metabolism of heme into carbon monoxide, ferrous iron, and biliverdin. Through biliverdin reductase, biliverdin becomes bilirubin. HMOX1 -deficiency is an exceedingly rare autosomal recessive disorder with hallmark features of direct antibody negative hemolytic anemia with normal bilirubin, hyperinflammation and features indicating hemophagocytosis lymphohistiocytosis. Clinical findings have included asplenia, nephritis, hepatitis, and evidence of vasculitis. Pulmonary features and evaluation of the immune response have been limited. Results Here, we present the fifth reported case in literature of a young boy who remarkably also presented with chronic respiratory failure due to nonspecific interstitial pneumonia in addition to infection-triggered recurrent hyperinflammatory flares notable for hemolysis without hyperbilirubinemia, immunodeficiency, hepatomegaly with mild transaminitis, asplenia, leukocytosis, thrombocytosis, joint pain and features of macrophage activation with negative autoimmune serologies. Lung biopsy revealed cholesterol granulomas. He was found post-mortem by whole exome sequencing to have a compound heterozygous paternal frame shift a paternal frame shift HMOX1 c.264delCTGG (p.L89Sfs*24) and maternal splice donor HMOX1 (c.636+2 T>A) consistent with HMOX1 deficiency. Western blot analysis confirmed lack of HMOX1 protein upon oxidant stimulation of the patient cells. Conclusions Here, we describe a phenotype expansion for HMOX1-deficiency to include not only asplenia and hepatomegaly, but also interstitial lung disease with cholesterol granulomas and inflammatory flares with hemophagocytosis present in the bone marrow.
The Antiasthma Medication Ciclesonide Suppresses Breast Cancer Stem Cells through Inhibition of the Glucocorticoid Receptor Signaling-dependent YAP Pathway
Authors: Kim Su-Lim , Choi Hack Sun , Kim Ji-Hyang , Lee Dong-Sun 
Published: February 2020

Abstract

Background Ciclesonide is an inhaled corticosteroid used to treat mild-to-severe asthma. However, whether it has anticancer and anti-cancer stem cell (CSC) effects is unknown. This study focused on studying the effect on breast cancer and breast CSCs by ciclesonide and determining its molecular mechanism. Methods The sensitivity of breast cancer by ciclesonide was determined by cell apoptosis, migration, colony formation, and xenograft. Effect of ciclesonide on CSC formation through GR/YAP pathway was determined by SiRNA of GR and YAP, GR degradation assay, GR antagonist, nuclear localization of GR and YAP, and YAP inhibitor. CSC subpopulation was determined by mammosphere culture, CD44+/CD24-, and aldefluor assay. Results Here, we showed that ciclesonide inhibits breast cancer and CSC growth. Similar glucocorticoids (GC), dexamethasone (DEX) and prednisone, did not suppress CSC formation. Ciclesonide-induced glucocorticoid receptor (GR) degradation was dependent on ubiquitination. We showed via GR small interfering RNA (siRNA) that GR plays a crucial role in CSC formation. We showed via western blot and immunofluorescence assays that ciclesonide reduces the nuclear level of GR. The GR antagonist RU-486 also inhibited CSC formation. Ciclesonide reduced the protein level of the Hippo transducer Yes-associated protein (YAP). GR siRNA induced a decrease in YAP protein expression and inhibited mammosphere formation. The YAP inhibitor verteporfin inhibited CSC formation and transcription of the connective tissue growth factor and cysteine-rich protein 61 genes. The GR/YAP1 pathway regulated breast CSC formation. Conclusions We describe that ciclesonide inhibition CSCs through GR and YAP signaling pathway. These findings suggest that the GR/YAP signaling pathway regulates breast CSC formation and revealed a new approach for targeting GR and YAP to inhibit CSC formation.
Assessing immune infiltration and the tumor microenvironment for the diagnosis and prognosis of sarcoma
Authors: Zhu Naiqiang , Hou Jingyi 
Published: February 2020

Abstract
Background Sarcomas, cancers originating from mesenchymal cells, are comprehensive tumors with poor prognoses, yet their tumorigenic mechanisms are largely unknown. In this study, we aimed to characterize infiltrating immune cells and genes associated with the immunologic response to sarcomas. Method The “CIBERSORT” algorithm was used to calculate the amount of L22 immune cell infiltration in sarcomas. Then, the “ESTIMATE” algorithm was used to assess the “Estimate,” “Immune,” and “Stromal” scores. Weighted gene co-expression network analysis (WGCNA) was utilized to identify the significant module related to the immune therapeutic target. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Gens and Genomes (KEGG) analysis were applied using the “clusterProfiler” package in R for annotation and visualization. Results Macrophages were the most common immune cells infiltrating sarcomas. The number of CD8 T cells was negatively associated with that of M0 and M2 macrophages, and positively associated with M macrophages in sarcomas samples. The clinical parameters (disease type, gender) significantly increased with higher Estimate, Immune, and Stromal scores, and with a better prognosis. The blue module was significantly associated with CD8 T cells. Functional enrichment analysis showed that the blue module was mainly involved in chemokine signaling and the PI3K-Akt signaling pathway. CD48, P2RY10 and RASAL3 were identified and validated at the protein level. Conclusion Based on the immune cell infiltration and immune microenvironment, three key genes were identified, which suggest novel molecular mechanisms of sarcoma metastasis.
Comparison of the factors associated with preference of life-sustaining treatment by caregivers’ and health facility tenants’ families: a cross-sectional study
Authors: Shaku Fumio , Tsutsumi Madoka , Nakamura Asumi , Takagi Hiroshi , Otsuka Takahiro , Maruoka Shuicihro 
Published: February 2020

Abstract
Background: Recently, advance care planning (ACP) in palliative care has been attracting attention in Japan. The Japanese counterpart was established by the Ministry of Health, Labor and Welfare in November 2018. Moreover, discussions on end-of-life treatment are an important part of ACP It is suggested that individual feelings at the end-of-life are different; ACP depends on various situations. Decision making by patients themselves is recommended; however, there are cases where patients with dementia or other disorders cannot make decisions by themselves. Health providers may contact surrogates and consider their background for better ACP. We focused on patents’ families and examined factors related to the preferences of home caregivers’ and old-age health facility tenants’ families on patient ACP, including life-sustaining treatment (LST) at end-of-life.Methods: This cross-sectional study involved home caregivers’ and old-age health facility tenants’ families in Japan. We distributed 925 self-reported questionnaires comprising items such as number of people living together, comprehension of doctor’s explanations, nursing care level and duration, the Patient Health Questionnaire (PHQ)-9 and Short Form (SF)-8, and caregivers’ LST preference.Results: A valid response was obtained from 619 respondents [242 men and 377 women (309 in the home-care group, response rate = 61.1%; 310 in the facility-care group, response rate = 74.0%)]. The mean age of the respondents was 65.29 ± 12.09 years in the home-care group and 63.24 ± 10.39 years in the facility-care group. LST preference was significantly associated with sex, number of people living together, care duration, and comprehension of doctors’ explanations in the home-care group but was not significantly associated in the facility-care group. Furthermore, PHQ-9/SF-8 scores were not significantly associated with LST preference of the groups.Conclusions: Factors associated with decision on ACP differed between home caregivers’ families and health facility tenants’ families. In ACP, healthcare providers should be aware of the nature of each patient’s caregiving situation and living environment when discussing LST preference.
Analisis Validitas dan Reliabilitas Alat Peraga Tabung Resonansi Horizontal beserta Instrumennya untuk Menunjang Keterampilan Generik Sains Siswa
Authors: Huda Choirul , Damayanti Feny Febriana , Nuvitalia Duwi 
Published: February 2020

<p class="Abstract">Penelitian ini bertujuan untuk menguji kelayakan instrumen dan alat peraga tabung resonansi horisontal untuk menunjang keterampilan generik sains siswa. Instrumen yang digunakan dalam penelitian ini adalah lembar observasi dengan jumlah 10 aktivitas siswa yang diamati. Media pembelajaran yang digunakan adalah produk berupa alat peraga tabung resonansi horisontal dan pedoman penggunaan alat. Penelitian ini merupakan penelitian deskriptif kualitatif untuk menilai dan mengetahui kualitas instrumen serta alat peraga untuk menunjang keterampilan generik sains siswa. Instrumen dan alat peraga masing-masing divalidasi oleh dua ahli pendidikan fisika. Hasil penelitian menunjukkan: 1) rata-rata hasil validasi oleh kedua ahli berada pada kriteria sangat valid dengan persentase masing-masing sebesar 85,42% untuk lembar observasi, 91,67% untuk alat peraga tabung resonansi horisontal, dan 100% untuk pedoman penggunaan alat; 2) reliabilitas produk berada pada kriteria reliabel dengan persentase instrumen lembar observasi 88,23%, alat peraga tabung resonansi horisontal 97,06%, dan pedoman penggunaan alat 100%. Berdasarkan hasil penelitian, instrumen dan alat peraga tabung resonansi horisontal layak digunakan untuk menunjang keterampilan generik sains siswa dengan kriteria sangat valid dan reliabel.</p>
Study on the lifetime of photocatalyst by photocatalytic membrane reactors (PMR)
Authors: Yan Xiaoju , Li Junyu , Ma Cong , Tang Yu , Kong Xiangji , Lu Jinfeng 
Published: February 2020

Abstract
The continuously photocatalytic degradation of methyl orange (MO) was carried out using a photocatalytic membrane reactor (PMR). The lifetime, cause of deactivation, and regeneration of Degussa P25 titanium dioxide (TiO2) were investigated. The photocatalyst was deactivated when the concentration of MO in the effluent of the PMR was stable. To characterize the lifetime of the photocatalyst, we applied g MO/g TiO2. The lifetime of the photocatalyst during the photocatalytic degradation of 10 mg/L MO was 3.71 times that of 5 mg/L MO. Changing the hydraulic retention time of the PMR from 0.75 to 3 h prolonged the lifetime of the photocatalyst. Deactivation of the photocatalyst was not due to pore blocking by the reactant (MO) or intermediate products. The surface adsorption of MO and the reaction intermediates deactivated the catalyst. The spent catalysts were regenerated after washing with methanol and hydrogen peroxide (H2O2) and then treated with heat. H2O2 treatment generated the highest regeneration rate, because H2O2 is a strong oxidizing agent that oxidized the deposited species on the surface of the photocatalyst.
Recent Progress in the Synthesis of Pyrroles
Authors: Xuan Duc Dau 
Published: February 2020

:
Pyrrole derivatives are nitrogen-containing heterocyclic compounds and widely
distributed in a large number of natural and non-natural compounds. These compounds
possess a broad spectrum of biological activities such as anti-infammatory, antiviral, antitumor,
antifungal, and antibacterial activities. Besides their biological activity, pyrrole derivatives
have also been applied in various areas such as dyes, conducting polymers, organic
semiconductors.

:
Due to such a wide range of applicability, access to this class of compounds has attracted
intensive research interest. Various established synthetic methods such as Paal-Knorr,
Huisgen, and Hantzsch have been modified and improved. In addition, numerous novel
methods for pyrrole synthesis have been discovered. This review will focus on considerable
studies on the synthesis of pyrroles, which date back from 2014.
Metode Bermain Peran dan Alat Permainan Edukatif untuk Meningkatkan Empati Anak Usia Dini
Authors: Rahmawati Anayanti 
Published: February 2020

Penelitian dengan judul Metode Bermain Peran Dan Alat Permainan Edukatif Untuk Meningkatkan Empati Anak Usia Dini ini bertujuan meningkatkan empati anak usia dini. Subjek penelitian yaitu anak kelompok B TK Darul Arqom Makamhaji, Kecamatan Kartasura, Kabupaten Sukoharjo . Penelitian ini menggunakan penelitian tindakan kelas sebanyak 2 siklus. Dengan membandingkan hasil observasi empati anak pada siklus I dan siklus II dapat dilihat terjadinya peningkatan empati. Hasil tersebut menunjukkan penggunaan metode bermain peran dan alat permainan edukatif di TK Darul Arqom dapat berpengaruh dan meningkatkan empati. Kata kunci: metode bermain peran, alat permainan edukatif, empaty
Pengembangan Instrumen Evaluasi Non Tes (Informal) untuk Menjaring Data Kualitatif Perkembangan Anak Usia Dini
Authors: Syamsudin Amir 
Published: February 2020

Tindakan anak, ucapan/ekspresi tubuh anak, dan hasil karya anak merupakan sumber informasi tentang perkembangan mental anak. Informasi yang bersumber dari tindakan anak dapat diperoleh menggunakan metode observasi. Informasi yang bersumber dari ucapan ekspresi tubuh anak dapat digali melalui metode wawancara. Sedangkan informasi yang bersumber dari hasil karya anak dapat diperoleh melalui metode dokumentasi. Observasi adalah aktivitas mencatat suatu gejala/peristiwa dengan bantuan alat/instrumen untuk merekam/mencatatnya guna tujuan ilmiah atau tujuan lainnya. Rekaman wawancara/percakapan adalah kumpulan informasi yang digali melalui tanya jawab lisan dan percakapan sehari-hari dengan tujuan untuk mengukur kadar pengetahuan yang sudah dimiliki anak. Dokumen merupakan catatan yang bersifat pribadi seperti buku harian, surat-surat, atau catatan lapangan. Dokumen sangat penting untuk menutup celah perbedaaan informasi yang digali dari wawancara dengan informasi yang digali dari observasi. Kata Kunci: instrumen evaluasi non tes, perkembangan anak
Pelatihan Penanganan Bullying Pada Anak TK
Authors: Muthmainnah Muthmainnah , Astuti Budi , Fatimaningrum Arumi Savitri 
Published: February 2020

Program ini bertujuan untuk meningkatkan pengetahuan guru TK dalam menangani bullying di TK. Khalayak sasaran dalam program ini adalah guru TK di wilayah Imogiri Bantul yang berjumlah 31 orang. Kegiatan ini dilaksanakan dengan metode ceramah, tanya jawab, pemberian tugas dan diskusi, danpresentasi. Pelatihan penanganan bullying pada anak TK dapat meningkatkan pengetahuan dan wawasan tentang pengertian bullying, jenis dan dan dampaknya bagi anak,serta penanganan yang tepat bagi pelaku, korban atau teman yang melihat tindak bullying serta orang tua. Hal ini dapat dilihat dari hasil evaluasi pelaksanaan kegiatan dan evaluasi penanganan masalah bullyingyang telah mencapai indikator keberhasilan melebihi 80 %. Berdasarkan hasil penyebaran skala tentang penyelenggaraan kegiatan dapat disimpulkan bahwa 100 % guru TK (31 peserta) merasa materi disampaikan dengan jelas dan sesuai serta bermanfaat bagi peserta. Berdasarkan rubrik penilaian dalam diskusi penanganan bullying, 5(lima) kelompok mencapai kriteria baik dalam merancang penanganan bullying, baik untuk pelaku bullying, korban bullying, teman yang melihat bullying maupun penanganan untuk orang tua. Kata Kunci: penanganan, bullying, anak TK