bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Anaplasma and Ehrlichia species–specific peptide ELISAs with whole organism–based immunofluorescent assays for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs
Authors: Qurollo Barbara A. , Stillman Brett A. , Beall Melissa J. , Foster Paulette , Hegarty Barbara C. , Breitschwerdt Edward B. , Chandrashekar Ramaswamy 
Published: December 2020

Abstract

OBJECTIVE
To compare the performance of 5 synthetic peptide–based ELISAs with that of 3 commercially available immunofluorescent assays (IFAs) for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs.

SAMPLE
A convenience set of 109 serum samples obtained before and at various times after inoculation for 23 dogs that were experimentally infected with Anaplasma phagocytophilum, Anaplasma platys, Ehrlichia canis, Ehrlichia chaffeensis, or Ehrlichia ewingii and 1 uninfected control dog in previous studies.

PROCEDURES
All serum samples were assessed with 5 synthetic peptide–based ELISAs designed to detect antibodies against A phagocytophilum, A platys, E canis, E chaffeensis, and E ewingii and 3 whole organism–based IFAs designed to detect antibodies against A phagocytophilum, E canis, and E chaffeensis. The species-specific seroreactivity, cross-reactivity with the other tick-borne pathogens (TBPs), and diagnostic sensitivity and specificity were calculated for each assay and compared among assays.

RESULTS
All serum samples obtained from dogs experimentally infected with a TBP yielded positive results on a serologic assay specific for that pathogen. In general, sensitivity was comparable between ELISAs and IFAs and tended to increase with duration after inoculation. Compared with the IFAs, the corresponding ELISAs were highly specific and rarely cross-reacted with antibodies against other TBPs.

CONCLUSIONS AND CLINICAL RELEVANCE
Results suggested that peptide-based ELISAs had enhanced specificity relative to whole organism–based IFAs for detection of antibodies against Anaplasma and Ehrlichia spp, which should facilitate accurate diagnosis and may help detect dogs coinfected with multiple TBPs.
Gambaran hasil spirometri pada wanita pedesaan Krueng Raya
Authors: Syahrizal Dedy , Andayani Novita , Aprilianti Rahmi 
Published: December 2020

Abstrak. Pemeriksaan spirometri adalah pemeriksaan yang dilakukan untuk mengukur volume dan kapasitas paru-paru. Pemeriksaan ini bertujuan untuk mengetahui kapasitas vital, kapasitas vital paksa, volume ekspirasi paksa dalam satu detik dan aliran pernafasan maksimal. Tujuan pada penelitian ini adalah untuk melihat gambaran hasil spirometri pada wanita pedesaan Krueng Raya, keterkaitan dengan keadaan lingkungan Krueng Raya terdapat pelabuhan pertamina dan pabrik semen. Penelitian ini dilakukan di Puskesmas Masjid Raya Krueng Raya pada tanggal 30 Agustus sampai dengan 16 September 2019 terhadap 40 pasien. Metode sampling non probability sampling dengan teknik accidental sampling. Pemeriksaan dilakukan dengan menggunakan alat spirometri vitalograph dengan menganalisis interpretasi hasil pemeriksaan. Hasil analisis didapatkan pada penelitian ini melibatkan wanita Krueng Raya (100%) pemeriksaan spirometri didapatkan hasil normal sebanyak 42.5%, restriksi 47.5%, obstruksi 5% dan restriksi dan obstruksi 5%. Analisis yang dilakukan secara univariat untuk melihat gambaran hasil spirometri dengan pengaruh faktor lain seperti lingkungan, BMI dan kebiasaan lainnya. Kata Kunci: Hasil Spirometri, Fungsi Paru, VEP1, KVPAbstract. Spirometry examination is an examination carried out to measure the volume and capacity of the lungs. This examination aims to determine the vital capacity, forced vital capacity, forced expiratory volume in one second and maximum respiratory flow. The purpose of this study was to look at the description of spirometry results in rural women in Krueng Raya, the relationship with Krueng Raya's environmental conditions contained Pertamina ports and cement factories. The research was conducted at the Krueng Raya Masjid Raya Health Center on 30 August to 16 September 2019 on 40 patients. Non-probability sampling method with accidental sampling technique. The examination is carried out using a vitalograph spirometry tool by analyzing the interpretation of the results of the examination. The analysis results obtained in this study involved Krueng Raya women (100%) spirometry examination showed normal results of 42.5%, 47.5% restriction, obstruction of 5% and restriction and obstruction of 5%. Univariate analysis is done to see the spirometry results with the influence of other factors such as the environment, BMI and other habits. Keywords: Spirometry Results, Lung Function, FEV1, FVC
Hubungan indeks massa tubuh dengan kejadian osteoartritis lutut di Rumah Sakit Umum Zainoel Abidin Kota Banda Aceh
Authors: Nata Cici Enjelia , Rahman Safrizal , Sakdiah Sakdiah 
Published: December 2020

Abstrak. Indeks Massa Tubuh (IMT) merupakan parameter yang digunakan untuk menilai komposisi tubuh dengan menggunakan klasifikasi Asia Pasifik. IMT menjadi salah satu faktor risiko terjadinya osteoartritis lutut. Osteoartritis lutut merupakan penyakit degeneratif yang terjadi pada sendi lutut. Berdasarkan RISKESDAS tahun 2018, Provinsi Aceh menduduki peringkat pertama sebagai provinsi dengan angka penyakit sendi terbanyak yang termasuk osteoartritis lutut yaitu 13.3%. Prevalensi indeks massa tubuh berlebih di Indonesia pada tahun 2018 meningkat dari 26.3% menjadi 34.4% sehingga kemungkinan terjadinya osteoartritis lutut juga meningkat. Penelitian ini menggunakan metode observasional analitik dengan desain cross-sectional dilakukan dengan cara mengukur berat badan dan tinggi badan responden yang memenuhi kriteria penelitian berdasarkan pedoman American College of Rheumatology (ACR). Sampel dikumpulkan berdasarkan rekam medis di Rumah Sakit Umum Zainoel Abidin kota Banda Aceh sejak september hingga oktober 2019 yang melibatkan tujuh puluh responden sebagai sampel penelitian yang dimana 51 responden (72.9%) memiliki indeks massa tubuh berlebih dan 43 responden (61.4%) didiagnosis dengan Osteoartritis lutut. Berdasarkan hasil analisis uji Chi Square, nilai p value yang diperoleh adalah 0.021 (p value ≤ 0,05). Dengan demikian, penelitian ini menunjukkan bahwa terdapat hubungan antara indeks massa tubuh dengan kejadian osteoartritis lutut di Rumah Sakit Umum Zainoel Abidin di kota Banda Aceh.Kata Kunci: Indeks massa tubuh; berat badan lebih, osteoartritis lutut; Abstract. Body Mass Index (BMI) is a parameter to measure body composition which is classified into underweight, normal and overweight. Overweight has been known to potentially cause knee osteoarthritis. Knee osteoarthritis is a degenerative disease happening on the knee. Based on RISKESDAS 2018, Aceh led Indonesia as a province with the highest number of knee arthritis (13.3%). In the same year, the prevalence of overweight in Indonesia has also increased from 26.3% to 34.4% and is expected to rise annually. This research is an analytic observational using cross sectional design. The method used in this research is body weight and body length measurement of samples fulfilling the criteria based on American College of Rheumatology (ACR). Data were collected based on medicalr record at Zainoel Abidin General Hospital, Banda Aceh, from September to October 2019. We examined 70 respondents, 51 of them (72.9%) had overweight IMT and 43 of them (61.4%) were diagnosed with knee osteoarthritis. Chi Square analysis showed p value 0,021 (p value ≤ 0.05). We concluded that there is a correlation between body mass index with knee osteoarthritis in Zainoel Abidin General Hospital, Banda Aceh.Keyword: Body Mass Index; overweight; knee osteoarthritis;
Perbedaan uji diagnostik antigen, antibodi, RT-PCR dan tes cepat molekuler pada Coronavirus Disease 2019
Authors: Yanti Budi , Ismida Fitri Dewi , Sarah Klarina Elsa Siti 
Published: December 2020

Coronavirus Disease 2019 (COVID-19) merupakan jenis penyakit baru yang teridentifikasi pada manusia. Virus penyebab COVID-19 ini dinamakan SARS-CoV-2. Angka mortalitas yang disebabkan oleh SARS-COV-2 (3,8%), hal ini lebih rendah dari angka mortalitas yang disebabkan oleh infeksi coronavirus sebelumnya, yaitu SARS-COV (10%) dan MERS-COV (37,1%). Namun, angka penularan dari SARS-COV-2 jauh lebih tinggi, yaitu 10 kali lipat bersifat lebih infeksius. Hal ini dapat dijadikan sebagai penjelasan penyebab dari mewabahnya virus yang terjadi secara mendadak. Infeksi SARS-COV-2 terjadi melalui droplets, kontak dengan cairan tubuh pasien yang terinfeksi, benda-benda yang terkontaminasi. COVID-19 dapat menimbulkan tanda dan gejala yang bervariasi. (WHO-2019-nCOV) Infeksi ini dapat menyebabkan gejala ISPA ringan hingga berat bahkan sampai terjadi Acute Respiratory Distress Syndrome (ARDS), sepsis dan syok septik. Dalam melakukan diagnosis diperlukannya pemeriksaan yang memiliki tingkat sensitifitas dan spesifisitas yang tinggi. Pemeriksaan penunjang yang dilakukan mencakup pemeriksaan radiologis, dan pemeriksaan laboratorium (uji antigen, antobodi, serologi dan molekuler). Dalam referat ini akan dibahas mengenai beberapa pemeriksaan diagnostik yang umum dilakukan saat ini, yaitu rapid test antigen, rapid test antibodi, ELISA, RT-PCR dan Tes Cepat Molekuler. Setiap pemeriksaan memiliki kelebihan dan kekurangannya masing-masing. Namun pemeriksaan baku emas dalam mendiagnosis COVID-19 tetap menggunakan RT-PCR.
PENAMAAN DESA DI KABUPATEN OGAN KOMERING ILIR (OKI) DALAM PERSEPSI TOPONIMI TERESTRIAL
Authors: Muhidin Rahmat 
Published: December 2020

Penamaan desa merupakan unsur penting dalam kehidupan manusia guna mengenali lingkungan di sekitarnya. Pemberian nama desa adalah bagian dari penamaan unsur rupabumi daratan atau terestrial yang perlu ditelusuri dan dibakukan penamaannya. Permasalahan dalam penelitian ini adalah: (1) Mengapa masyarakat Kabupaten Ogan Komering Ilir (OKI) dalam menamai suatu desa berkaitan dengan pengalaman empiris yang dialaminya? (2) Hal-hal apa sajakah yang melatarbelakangi pola pikir masyarakat Kabupaten OKI dalam menamai desa di lingkungannya? Penelitian ini bertujuan menggali nama geografis dan mendeskripsikan penamaan desa di Kabupaten OKI berdasarkan kajian toponimi terestrial. Penelitian ini bersifat deskriptif kualitatif. Metode pengumpulan data dengan teknik wawancara dan penjaringan data melalui penyebaran kuesioner pada informan. Hasil kajian menunjukkan bahwa penamaan desa di Kabupaten OKI berdasarkan persepsi toponimi terestrial yang mengacu pada sumber daya alam terestrial dan sumber daya marine (maritim). Penamaan desa cenderung mengacu/ berasal pada: (1) nama orang; (2) nama tumbuhan; (3) unsur nama geografis, dan (4) unsur harapan. Unsur geografis antara lain meliputi: karang, kebon, kuala, lalang, muara, pulau, rantau, rawa, rimba, tanah, talang, telang, sungai, upang, tebing, teluk, tanjung, pematang, pangkalan, dan lubuk. Penamaan desa di Kabupaten OKI cenderung menggunakan bahasa Melayu, bahasa Komering, dan bahasa Jawa.
Interconnection of perfection and success in learning foreign languages
Authors: Savina O. Yu. 
Published: December 2020

The article deals with the relationship between perfectionism in the academic environment and foreign language learning. After defining the basic concepts (the three-dimensional model of perfectionism, its components, the multidimensional perfectionism scale by P. Hewitt and G. Flett), the results of the current related research conducted abroad and in Russia are briefly presented. The literature review allowed us to formulate a research question: Are the foreign language and its level of proficiency (on the Common reference levels) related to the degree of perfectionism and its components?In search of an answer to this question, an empirical study was conducted (sample size 213 participants). Using objective statistical methods, it was found that, in general, there are no statistically significant differences between students studying different languages and students at different levels. However, differences in the degree of perfectionism between A2 level and neighboring levels attract attention in terms of the effect sizes. For individual components of perfectionism, the levels of Basic user (A1 and A2) differ most from other levels.The presented results can be useful both for foreign language teachers and for scientists dealing with the success of language learning and the factors affecting this process.
Associação entre neuropatia diabética e sintomas de trato urinário inferior nas pessoas com diabetes mellitus: revisão integrativa
Authors: Costa Heloneida Maria Leoncio Mahle , Popovicz Débora , Paes Robson Giovani , Assis Gisela Maria , Esmanhotto Tatiane Coradassi , Erzinger Ana Rotília 
Published: December 2020

Objetivo: Investigar a associação entre neuropatia diabética e sintomas do trato urinário inferior nas pessoas com diabetes mellitus. Métodos: Revisão integrativa da literatura, que seguiu seis etapas; a busca ocorreu no mês de outubro de 2019, no Portal da Biblioteca Virtual de Saúde e nas bases de dados National Library of Medicine, Medical Literature Analysis and Retrieval System Online e Scopus, utilizando os descritores e operadores booleanos “urinary incontinence” AND “diabetes mellitus” AND “diabetic neuropathies”; os critérios de inclusão foram publicações de 2009 a 2019, nos idiomas português, inglês e espanhol. Resultados: Dos 52 estudos provenientes da busca, oito compuseram a amostra final; as mulheres foram majoritárias nos estudos; a neuropatia diabética estava associada aos sintomas de trato urinário inferior, sendo a incontinência urinária, incontinência de urgência, noctúria, bexiga hiperativa e disfunção sexual feminina; a obesidade, cor branca, tempo e descontrole da doença foram fatores associados aos sintomas urinários. Considerações finais: A avaliação da sensibilidade protetiva dos pés e detecção precoce de neuropatias, oferecem uma oportunidade para a prevenção dos sintomas de trato urinário inferior. As limitações deste estudo foram a heterogeneidade metodológica dos artigos, a estratégia de busca e a falta de dados com relação ao sexo masculino.
Availability, cost, and affordability of asthma and chronic obstructive pulmonary disease medications in The Gambia
Authors: Sanyang Baboucarr , Jagne Emily , Sefa Nana , Touray Sunkaru 
Published: December 2020

Objectives:Guidelines for asthma and chronic obstructive pulmonary disease (COPD) have undergone significant changes. Specifically, inhaled corticosteroids (ICSs) either alone or in combination with a long-acting beta2-agonist (LABA) are now first-line treatment for asthma, while long-acting muscarinic antagonists alone or in combination with LABAs are first-line treatment of COPD. Data on local availability, cost, and affordability of these medicines in The Gambia are unknown.Materials and Methods:We surveyed all registered pharmacists in Gambia and calculated affordability indices relative to the prevailing wage of the lowest government worker.Results:Eight out of 18 registered pharmacists responded to the survey. Respondents were responsible for stocking 19 out 26 registered pharmacies in the country, resulting in a response rate of 44%. Salbutamol inhalers were widely available, stocked by the central medical stores and 6 out of 8 surveyed pharmacists. Only one pharmacist reported stocking beclometasone 50 mcg, budesonide 100 mcg, and fluticasone propionate 125 mcg inhalers. Aminophylline was stocked by 4/8 pharmacists. The price of salbutamol 100 mcg inhaler was Gambian Dalasi (GMD) 200 (US$ 4, 4 days’ wages), while ipratropium bromide 20 mcg cost GMD 675 (US$ 14, 15 days’ wages). ICS maintenance inhalers at private pharmacies cost 15, 26, and 28 days’ wages for beclomethasone 50 mcg, fluticasone propionate 125 mcg, and budesonide 100 mcg, respectively. Combination of ICS/LABA inhaler was 26 days’ wages while tiotropium bromide 18 mcg affordability was 95 days’ wages.Conclusion:Guideline recommended medications for the management of asthma and COPD in The Gambia are unavailable, expensive, and unaffordable.
Monitoring of brightness temperature fluctuation of water in SHF range
Authors: Иванов Ю.Д. , Козлов А.Ф. , Галиуллин Р.А. , Татур В.Ю. , Веснин С.Г. , Зиборов В.С. , Иванова Н.Д. , Плешакова Т.О. 
Published: December 2020

Цель исследования - в мониторинге флуктуаций Т воды в процессе ее испарения при температуре t = 42°С, критической для человека, с помощью СВЧ-радиометрии. Методика: проводился мониторинг изменения яркостной температуры Т воды в СВЧ-диапазоне частот 3,8-4,2 ГГц в процессе ее испарения при температуре в измерительной конусной полипропиленовой кювете t = 42°С. Измерения яркостной температуры проводились при помощи радиотермометра. Результаты. Обнаружено появление скачка яркостной температуры Т при температуре в измерительной конусной полипропиленовой кювете = 42°С. Скачок Т характеризовался фронтом нарастания яркостной температуры в этом диапазоне частот в диапазоне ~4°С с градиентом ~0,05°С/мин - 15°С/мин в зависимости от условий организации процесса испарения и резким спадом в течение 10 с, после чего наблюдалась следующая серия менее интенсивных флуктуаций. При этом температура воды оставалась постоянной. Заключение. Выявлены существенные изменения яркостной температуры воды в СВЧ-диапазоне при ее испарении при температуре воды в конусной измерительной кювете t = 42°С, наблюдается флуктуация в виде скачка Т порядка DТ ~4°C в исследуемой области температуры флуктуаций. При этом термодинамическая температура практически не изменяется. Наблюдаемые эффекты должны учитываться при разработке диагностических систем патологического состояния человека и при создании аналитических устройств.
The purpose of the research consisted in detection of fluctuation of brightness temperature (T) of water in the area of the temperature Т = 42°С (that is critical for human) during its evaporation by SHF radiometry. Methods: Monitoring of the changes in brightness temperature of water in superhigh frequency (SHF) range (3.8-4.2 GHz) near the phase transition temperature of water Т = 42°С during its evaporation in the cone dielectric cell. The brightness temperature measurements were carried out using radiometer. Results: Fluctuation with maximum of brightness temperature was detected in 3.8-4.2 GHz frequency range near at the temperature of water Т = 42°С. It was characteristic for these T fluctuations that brightness temperature rise time in this range of frequencies in ~4°С temperature range with 0.05-15°С/min gradient and a sharp decrease during 10 s connected with measuring vapor conditions. Then nonintensive fluctuation series was observed. At that, the environment temperature remained constant. Conclusion: The significant increasing in brightness temperature of water during its evaporation in SHF range near the temperature of Т ~42°С were detected. It was shown that for water, Т pull with the amplitude DТ ~4°C are observed. At the same time, thermodynamic temperature virtually does not change. The observed effects can be used in the development of the systems for diadnostics of pathologies in human and analytical system.
Молекулярная биология менингиом головного мозга
Authors: Бывальцев В.А. , Степанов И.А. , Белых Е.Г. , Яруллина А.И. 
Published: December 2020

Цель обзора - анализ современных данных литературы о нарушении внутриклеточных сигнальных путей, играющих ведущую роль в развитии менингиом, генетических и молекулярных профилях данной группы опухолей. К настоящему времени изучено множество аберрантных сигнальных внутриклеточных путей, которые играют важнейшую роль в развитии менингиом головного мозга. Четкое понимание поврежденных внутриклеточных каскадов поможет изучить влияние генетических мутаций и их эффектов на менингиомогенез. Подробное исследование генетического и молекулярного профиля менингиом позволит сделать первый уверенный шаг в разработке более эффективных методов лечения данной группы интракраниальных опухолей. Хромосомы 1, 10, 14, 22 и связанные с ними генные мутации ответственны за рост и прогрессию менингиом. Предполагается, что только через понимание данных генетических повреждений будут реализованы новейшие эффективные методы лечения. Будущая терапия будет включать в себя комбинации таргетных молекулярных агентов, в том числе генную терапию, малые интерферирующие РНК, протонную терапию и другие методы воздействия, как результат дальнейшего изучения генетических и биологических изменений, характерных для менингеальных опухолей.Meningiomas are by far the most common tumors arising from the meninges. A myriad of aberrant signaling pathways involved with meningioma tumorigenesis, have been discovered. Understanding these disrupted pathways will aid in deciphering the relationship between various genetic changes and their downstream effects on meningioma pathogenesis. An understanding of the genetic and molecular profile of meningioma would provide a valuable first step towards developing more effective treatments for this intracranial tumor. Chromosomes 1, 10, 14, 22, their associated genes, have been linked to meningioma proliferation and progression. It is presumed that through an understanding of these genetic factors, more educated meningioma treatment techniques can be implemented. Future therapies will include combinations of targeted molecular agents including gene therapy, si-RNA mediation, proton therapy, and other approaches as a result of continued progress in the understanding of genetic and biological changes associated with meningiomas.
Matrix metalloproteinases in stroke
Authors: Пальцын А.А. 
Published: December 2020

Матриксные металлопротеиназы - ферментный комплекс, необходимый для сохранения гомеостаза. Он участник нормальной, постоянно текущей реконструкции всех живых тканей. Действие патогенных факторов нарушает слаженную работу этого комплекса. Часто нарушение выражается излишней активностью ферментов, усиливающей патогенное действие. Однако и заживление, форсированное новообразование тканевых элементов, может происходить только при повышенной, в сравнении с нормой, активности металлопротеиназ. Такая ситуация требует от медицины умения разумно вмешиваться в работу ферментной системы. В статье представлены некоторые результаты этих вмешательств.Matrix metalloproteinases - enzyme complex necessary for maintenance of the homeostasis. He is a participant of normal, constantly current reconstruction of all living tissues. Action of pathogenic factors breaks harmonious work of this complex. Often violation is expressed by the excessive activity of enzymes amplifying pathogenic action. However and healing, which is accelerated new growth of tissue elements, can happen only at raised, compared with norm, metalloproteinase activity. Such situation demands from medicine of ability participate reasonably in work of enzyme system. The article presents some of the results of these actions.