bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative analysis of the driving factors for groundwater resource changes in arid irrigated areas
Authors: Guo Chenyu , Liu Tie , Niu Yaxuan , Liu Zhibin , Pan Xiaohui , De Maeyer Philippe 
Published: November 2020

AbstractHow to quantify the impact of climate change and human activities on groundwater is not only a hot topic of current research but also a key point of water resource management in arid irrigated areas. Therefore, this paper analyzes the changes in the trends of land use, climate, and groundwater extraction in the Yanqi Basin in recent years and uses the distributed hydrological model MIKE‐SHE to quantitatively analyze the impacts of these three factors on groundwater resources. The results show that: 1. The Nash coefficients of the simulated and observed groundwater levels during the verification period are 0.84, 0.79 and 0.76; the correlation coefficient between the simulated and observed soil moisture is 0.86. Although there are some uncertainties in the simulation, the results prove that the model can be used to simulate arid irrigated areas. 2. The effects of these three factors on groundwater levels are 5, 12.5 and 82.5%, respectively, and have caused the regional average groundwater level to decrease by a maximum of 0.07, 0.23 and 1.79 m, respectively. The effects of these three factors on the interactions between surface water and groundwater were 7.04, 3.63 and 89.33%. Groundwater extraction has become the main influencing factor of regional groundwater resources changes due to its more direct influence. 3. The influence of groundwater extraction has a strong spatial distribution characteristic and 10% of the study area has been greatly impacted by the groundwater extraction. Base on the above results, integrating multidisciplinary knowledge to establish the relationship between ecological environment and groundwater changes can provide strategies for the sustainable development of groundwater.
MicroRNA‐378a‐3p contributes to ovarian cancer progression through downregulating PDIA4
Authors: Chanjiao Yao , Chunyan Chen , Xiaoxin Qiu , Youjian Han 
Published: November 2020

AbstractObjectiveMicroRNAs, as essential players in tumorigenesis, have been demonstrated to have a revolutionary effect on human cancer research. Ovarian cancer is the primary reason of death among gynecologic malignancies. In view of this, it is significant to identify prognostic and predictive markers for treatment of ovarian cancer. The aim of this study was to probe into the effects of miR‐378a‐3p and protein disulfide‐isomerase A4 (PDIA4) on the biological functions of ovarian cancer cells.MethodsmiR‐378a‐3p expression and PDIA4 messenger RNA expression in human ovarian cancer cells, normal human ovarian epithelial cells, and serum of both ovarian cancer patients and healthy people were detected by reverse transcription‐quantitative polymerase chain reaction, and the PDIA4 protein expression was tested by Western blot analysis. Ovarian cancer OVCAR3 and SKOV3 cells were transfected or cotransfected with miR‐378a‐3p mimic or pcDNA3.1‐PDIA4 or their negative control plasmids to explore their roles in biological functions in ovarian cancer cells. Luciferase activity and RIPA assays were implemented to validate the interaction between miR‐378a‐3p and PDIA4. Western blot analysis was utilized to detect phosphatidylinositol‐3 kinase/serine/threonine kinase (PI3K/AKT) signaling pathway‐related protein expression and their phosphate expression levels.ResultsmiR‐378a‐3p was elevated and PDIA4 was decreased in ovarian cancer cells and serum. In addition, miR‐378a‐3p mimic induced ovarian cancer cell growth, while miR‐378a‐3p inhibitor and pcDNA3.1‐PDIA4 presented an inverse trend. pcDNA3.1‐PDIA4 partially eliminated the capabilities of miR‐378a‐3p mimic on ovarian cancer progression. Meanwhile, miR‐378a‐3p was found to negatively regulate PDIA4, and miR‐378a‐3p mimic increased the phosphorylation levels of AKT and PI3K, while pcDNA3.1‐PDIA4 exhibited an opposite tendency. Furthermore, pcDNA3.1‐PDIA4 largely eliminated the functions of miR‐378a‐3p mimic on phosphorylation levels of AKT and PI3K.ConclusionThis study provides evidences that miR‐378a‐3p activates PI3K/AKT signaling pathway by modulating PDIA4 expression, thereby playing a role in promoting the growth of ovarian cancer cells. This study provides novel directions for targeted therapy of ovarian cancer.
The role of abnormal cerebroplacental ratio in predicting adverse fetal outcome in pregnancies with scheduled induction of labor
Authors: Günay Taner , Bilir Reyhan A. , Hocaoğlu Meryem , Bör Ergül D. , Özdamar Özkan , Turgut Abdulkadir 
Published: November 2020

AbstractObjectiveTo investigate the role of abnormal cerebroplacental ratio (CPR) in predicting adverse fetal outcome in pregnancies with induction of labor.MethodsThis prospective observational study conducted at Medeniyet University Göztepe Training and Research Hospital between December 1, 2018 and October 31, 2019 enrolled women with scheduled induction of labor at or beyond 37 weeks of pregnancy. Women with singleton non‐anomalous fetuses with cephalic presentation and who had Bishop scores of 5 or less in pelvic examination were included in the study. Exclusion criteria were the presence of uterine scar history, non‐cephalic presentation, multiple pregnancy, vacuum‐ or forceps‐assisted delivery, and shoulder dystocia. Using fetal Doppler ultrasound, CPR was calculated (the ratio of umbilical artery to middle cerebral artery pulsatility index) and categorized into abnormal CPR (<1) and normal CPR (≥1). Data on maternal and delivery characteristics, fetal birth weight, and fetal complications were compared between the groups.ResultsA total of 145 women were included, 28 in the abnormal CPR group and 117 in the normal CPR group. Multivariate analysis revealed labor induction at a later week of pregnancy (odds ratio [OR] 10.33, P = 0.001), lack of intrauterine growth restriction (IUGR) (OR 13.21, P = 0.001), fetal distress (OR 8.14, P = 0.003) or meconium aspiration (OR 159.91, P = 0.001), and umbilical artery pH values greater than 7.31 (OR 17.51, P = 0.015) to be associated with an increased likelihood of having normal (≥1) CPR values. Receiver operating characteristic analysis revealed association of normal CPR values with later labor induction (cut‐off value of >38.3 weeks, P = 0.001), higher birth weight (cut‐off value of >2460 g, P = 0.022) and higher umbilical artery pH (cut‐off value of >7.31, P = 0.007).ConclusionOur findings revealed the significant role of abnormal CPR in predicting adverse fetal outcome in singleton pregnancies with scheduled induction of labor.
Soy isoflavone genistein inhibits hsa_circ_0031250/miR‐873‐5p/FOXM1 axis to suppress non‐small‐cell lung cancer progression
Authors: Yu Yaying , Xing Yanwei , Zhang Qian , Zhang Qianqian , Huang Shuangjian , Li Xinxin , Gao Chao 
Published: November 2020

AbstractThe foods of plants provide the rich nutrition and have protective function in human diseases, including cancers. Genistein is a major isoflavone constituent in soybeans, which has an anti‐cancer role in non‐small‐cell lung cancer (NSCLC). Nevertheless, the mechanism underlying the anti‐cancer function of genistein in NSCLC remains largely unknown. NSCLC cells (H292 and A549) were exposed to genistein. Circular RNA hsa_circ_0031250 (circ_0031250), microRNA (miR)‐873‐5p and forkhead box M1 (FOXM1) abundances were examined via quantitative reverse transcription polymerase chain reaction and Western blotting. The function of genistein, circ_0031250, miR‐873‐5p, and FOXM1 on NSCLC progression was investigated via Cell Counting Kit‐8, colony formation, transwell well, wound healing, flow cytometry, Western blotting and xenograft model. The target relationship was analyzed by dual‐luciferase reporter analysis and RNA immunoprecipitation. Results showed that genistein inhibited NSCLC cell viability in dose‐time‐dependent patterns. circ_0031250 abundance was elevated in NSCLC samples and cell lines, and it was reduced via genistein exposure. circ_0031250 knockdown aggravated genistein‐caused suppression of cell proliferation, migration and invasion and elevation of apoptosis. miR‐873‐5p expression was decreased in NSCLC samples and cells. miR‐873‐5p was targeted via circ_0031250, and miR‐873‐5p knockdown attenuated the influence of circ_0031250 silence on NSCLC progression in the presence of genistein. FOXM1 was regulated via circ_0031250/miR‐873‐5p axis. miR‐873‐5p constrained cell proliferation, migration and invasion and increased apoptosis via regulating FOXM1 in genistein‐treated cells. circ_0031250 knockdown enhanced the inhibitive function of genistein on NSCLC cell growth in xenograft model. Collectively, genistein repressed NSCLC progression by modulating circ_0031250/miR‐873‐5p/FOXM1 axis.
Circular RNA profiling facilitates the diagnosis and prognostic monitoring of breast cancer: A pair‐wise meta‐analysis
Authors: Ma Yanqing , Niu Xiaobin , Yan Sha , Liu Yuchun , Dong Ruihua , Li Yongwei 
Published: November 2020

AbstractBackgroundAs circular RNAs (circRNAs) have been found to significantly involve in the onset and progression of multiple malignant tumors including breast cancer (BC), this study aims at evaluating the diagnostic and prognostic values of circRNAs in this malady.MethodsAvailable databases were thoroughly searched to collect studies on the diagnosis and/or prognosis of BC using circRNA profiling. The updated Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS‐2) tool and the Newcastle Ottawa Scale (NOS) were used to assess the underlying bias of included studies. Clinical characteristics of the studies were merged by the quantitative‐weighted integral method to obtain the combined effects.ResultsSixteen studies were included, comprising 2438 BC cases and 271 noncancerous controls. The expression signature covered 24 circRNAs (down‐regulated: circ‐VRK1, hsa_circ_0068033, hsa_circ_103110, hsa_circ_104689, and hsa_circ_104821; up‐regulated: circAGFG1, hsa_circ_0001785, hsa_circ_0108942, hsa_circ_0001785, hsa_circ_006054, hsa_circ_100219, hsa_circ_406697, circEPSTI1, circANKS1B, circGFRA1, circ_0103552, CDR1‐AS, has_circ_001569, hsa_circ_001783, circFBXL5, circ_0005230, circAGFG1, circ‐UBAP2, and circ_0006528). The sensitivity and specificity of circRNAs in distinguishing BC patients from noncancerous controls were 0.65 and 0.68, and the corresponding area under the curve was 0.66. Survival analysis revealed that patients showing highly expressed oncogenic circRNAs were associated with increased mortality risks of BC in overall survival (univariate analysis: hazard ratio [HR] = 3.30, P = .000; multivariate analysis: HR = 3.07, P = .000), and disease‐free survival (HR = 8.26, P = .000). Stratified analysis based on circRNA expression status and control type also showed robust results.ConclusionsCircular RNA profiling presents prominent diagnostic and prognostic values in BC, and can be rated as a promising tool facilitating its early diagnosis and survival.
Neonatal carnitine concentrations in relation to gestational age and weight
Authors: Crefcoeur Loek L. , de Sain‐van der Velden Monique G. M. , Ferdinandusse Sacha , Langeveld Mirjam , Maase Rose , Vaz Frédéric M. , Visser Gepke , Wanders Ronald J.A. , Wijburg Frits A. , Verschoof‐Puite Rendelien K. , Schielen Peter C. J. I. 
Published: November 2020

AbstractBackgroundFree carnitine has been measured in the Dutch newborn screening (NBS) program since 2007 with a referral threshold of ≤5 μmol/L, regardless of gestational age or birthweight. However, several studies suggest that carnitine concentrations may depend on gestational age and birthweight. We evaluated differences in postnatal day‐to‐day carnitine concentrations in newborns based on gestational age (GA) and/or weight for GA (WfGA).MethodsA retrospective study was performed using data from the Dutch NBS. Dried blood spot (DBS) carnitine concentrations, collected between the 3rd and 10th day of life, of nearly 2 million newborns were included. Individuals were grouped based on GA and WfGA. Median carnitine concentrations were calculated for each group. Mann‐Whitney U tests, and chi‐square tests were applied to test for significant differences between groups.ResultsPreterm, postterm, and small for GA (SGA) newborns have higher carnitine concentrations at the third day of life compared to term newborns. The median carnitine concentration of preterm newborns declines from day 3 onwards, and approximates that of term newborns at the sixth day of life, while median concentrations of postterm and SGA newborns remain elevated at least throughout the first 10 days of life. Carnitine concentrations ≤5 μmol/L were found less frequently in SGA newborns and newborns born between 32 and 37 weeks of gestation, compared to term newborns.ConclusionsMedian carnitine concentrations in NBS DBS vary with day of sampling, GA, and WfGA. It is important to take these variables into account when interpreting NBS results..
Active biopolymer films based on furcellaran, whey protein isolate and Borago officinalis extract: characterization and application in smoked pork ham production
Authors: Zając Marzena , Jamróz Ewelina , Guzik Paulina , Kulawik Piotr , Tkaczewska Joanna 
Published: November 2020

AbstractBACKGROUNDThe meat industry is determined to find biodegradable packaging with properties similar to plastic. Furcellaran (FUR) and whey protein isolate (WPI) were used as a film matrix in which Borago officinalis extract (BOE) was incorporated as an antioxidant compound. The film's mechanical properties, water behavior, surface color, and antioxidant power were analyzed.Smoked hams were manufactured using two different types of film application: cured meat covered with film, smoked and cooked or hamsafter smoking, cooking and cooling. Smoked, vacuum packed ham was used as a control sample. The products were stored at 4 °C for 21 days and analyzed every 7 days.RESULTSThe elongation at break (EAB) and tensile strength (TS) of FUR/WPI films without the extract were 6.30% and 20.59 MPa, respectively, and after incorporating BOE, the EAB and TS were 24.30% and 15.33 MPa, respectively. The films with BOE were darker and had greater antioxidant capacity. The water content and activity in the products with films decreased along with storage time while the control remained stable. The results of microbiological, oxidation product accumulation, and sensory analysis were comparable in all the products.CONCLUSIONSThe smoking time can be reduced due to the dark color of the hams covered with BOE film. The barrier properties of those films should be increased. Other parameters were comparable to plastic packaging. The films therefore have the potential to be used instead of plastic packaging in the meat industry. © 2020 Society of Chemical Industry
The fermentation properties and microbial diversity of soy sauce fermented by germinated soybean
Authors: Zhao Guozhong , Li Jingjing , Zheng Fuping , Yao Yunping 
Published: November 2020

AbstractBACKGROUNDThe quality of commercial soy sauce is variable at present. Technical work is needed to improve the quality and flavor of soy sauce, especially in China. Material is a factor for influencing soy sauce characters in fermentation.RESULTSGerminated soybean sauce (fermented by germinated soybean) had a gamma aminobutyric acid (GABA) concentration of 6.83 μg mL−1, whereas a control (a soy sauce fermented by soybean) had a GABA concentration of less than 2.42 μg mL−1. Germinated soybean sauce also contained significantly higher levels of isoflavones, total polyphenol, and amino acid nitrogen than the control soy sauce. Microbial diversity results showed that Bacillus was the dominant bacteria in germinated soy sauce compared with the control. Aldehydes, alcohols, esters, and phenols were the major flavor components of germinated soybean sauce.CONCLUSIONA soy sauce with high levels of GABA, isoflavones, and total polyphenol was developed using germinated soybean. Stenotrophomonas, the typical pathogen found in the control, was reduced dramatically and replaced by Bacillus during fermentation with the germinated soybean. The germinated soybean sauce exhibited a better aroma and taste than the control. Soy sauce fermented by soybeans that germinated for 48 h exhibited greater advantages than soy sauce that germinated for 24 and 72 h. © 2020 Society of Chemical Industry
Chemical and microbial characterization for fermentation of water‐soluble cellulose acetate in human stool cultures
Authors: Yamada Hiroaki , Watabe Yuto , Suzuki Yutaka , Koike Satoshi , Shimamoto Shu , Kobayashi Yasuo 
Published: November 2020

AbstractBACKGROUNDWater‐soluble cellulose acetate (WSCA), a synthetic fiber source, was applied to human stool cultures and to pure cultures of representative Bacteroides species to characterize the fermentation properties of WSCA in the human gut, and to assess the potential availability of WSCA as a food or additive candidate.RESULTSAll nine of the different types of WSCA tested here provided increased acetate levels in human stool cultures. Greater levels of deacetylation were observed as the degree of substitution of hydroxyl groups by acetyl groups decreased. Among the nine tested types of WSCA, CA‐0.78‐128 caused the largest shifts of the microbial community, including an increased abundance of members of the genus Bacteroides, especially Bacteroides uniformis. Of four representative human gut Bacteroides species, only B. uniformis grew in pure culture on WSCA to produce acetate actively.CONCLUSIONWater‐soluble cellulose acetate has the potential for dietary application in human and other monogastric animals, based on the enhanced production of short‐chain fatty acids (SCFAs), in particular acetate, in the hindgut. Short‐chain fatty acid production is caused by selective proliferation of specific gut bacteria belonging to the genus Bacteroides. © 2020 Society of Chemical Industry
Biogenic amines: formation, action and toxicity – a review
Authors: Wójcik Wojciech , Łukasiewicz Monika , Puppel Kamila 
Published: November 2020

AbstractBiogenic amines (BA) are organic compounds commonly found in food, plants and animals, as well as microorganisms that are attributed with the production of BAs. They are formed as an effect of a chemical process: the decarboxylation of amino acids. Factors determining the formation of BAs include the availability of free amino acids and the presence of microorganisms that show activity with respect to carrying out the decarboxylation process. On the one hand, BAs are compounds that are crucial for maintaining cell viability, as well as the proper course of the organism's metabolic processes, such as protein synthesis, hormone synthesis and DNA replication. On the other hand, despite their positive effects on the functioning of the organism, an excessive content of BAs proves to be toxic (diarrhea, food poisoning, vomiting, sweating or tachycardia). Moreover, they can accelerate carcinogenesis. Amines are a natural component of plant and animal raw materials. As a result of the proven negative effects of amines on living organisms, the reduction of these compounds should be the subject of scientific research. The present review aims to synthesize and summarize the information currently available on BAs, as well as discuss the interpretation of the results. © 2020 Society of Chemical Industry
Effects of operating conditions on biogas production in an anaerobic digestion system of the food and beverage industry
Authors: Chen Hsinjung , Hung Jui‐Min , Hsu Kuo‐Chiang , Chuang Pei‐Ting , Chen Chin‐Shuh 
Published: November 2020

AbstractBACKGROUNDFood residuals (FR) were anaerobically biotransformed to produce biogases (e.g. methane and hydrogen), and different pre‐treatment conditions, including particle size, oil content, pH and salt content, were controlled in this study. The bio‐solids of a municipal solid waste (MSW) from a wastewater treatment plant were added to assess its effect on anaerobic transformation efficiency and gas yields.RESULTSThe breaking of FR and the application of MSW were effective in enhancing the transformation efficiency and yield of biogases. The energy transfer efficiency value of the combined FRs used in this study was probably 23%. However, it can be very cost effective to apply arbitrary proportions to treat two types of FR in the anaerobic digestion tank of a wastewater treatment plant. It was also found that the alkalinity and pH value were two major parameters that controlled the success of the transformation. About 0.16–0.17 kg of alkalinity was needed during the anaerobic digestion of 1 kg dry FR, but this requirement was decreased by the treatment applying MSW. Olive oil had higher reducing rates when used as a substitute for heat‐oxidized oil to study the effect of oil content on methylation.CONCLUSIONThe conditions for anaerobic digestion established in this study were practical for the digestion of FR in wastewater treatment plants in Taiwan. However, we nonetheless found that it was cost effective to use arbitrary proportions for both types of FR and integrate the anaerobic digestion process used in wastewater treatment plants. © 2020 Society of Chemical Industry
Sleeve lobectomy versus lobectomy for primary treatment of non‐small–cell lung cancer: A single‐center retrospective analysis
Authors: Zalepugas Donatas , Koryllos Aris , Stoelben Erich , Ludwig Corinna 
Published: November 2020

AbstractBackground and ObjectivesIt is unclear how much additional perioperative risk a sleeve lobectomy could pose in comparison to lobectomy. The objective of this analysis was to compare the complication rate, 30‐day mortality, and overall survival between lobectomy and sleeve lobectomy without prior neoadjuvant treatment in non‐small–cell lung cancer (NSCLC).MethodsThis is a retrospective study using our prospective database for quality assurance in our hospital. Inclusion criteria for our study was a completed lobectomy or sleeve lobectomy for primary treatment of NSCLC.ResultsIn 506 patients, the tumor was treated by means of standard lobectomy. In 252 patients with central tumor localization, sleeve lobectomy was performed. Postoperative complications occurred in n:148 (29.24%) patients of the lobectomy group and in n = 76 (30.15%) of the sleeve group. The mortality rate difference between the two groups was statistically significant and favored the lobectomy group (0.78% vs. 4.76%, p = .007). Five year survival was 69.97% for the lobectomy and 65.59% for the sleeve group (p = .829).ConclusionSleeve lobectomy for primary surgical treatment of NSCLC has comparable perioperative complications with lobectomy. Sleeve lobectomy does not seem to negatively influence survival. Postoperative mortality was higher in the sleeve group.