bluuebunny/crossref_metadata_2025_split huggingface dataset:
Yes SIRS or No SIRS? A Complication Following Cosmetic Medical Tourism
Authors: Lineberry Kyle , Punjabi Ayesha , Becker Devra B. 
Published: February 2020

Background: Fat transfer for buttock augmentation has become popular in the last quarter century. Increasingly, patients obtain the surgery internationally and return home in the early post-operative period. Complications after procedures performed internationally can be difficult to manage due to a paucity of medical and surgical history as well as challenges in obtaining salient records such as operative reports or communicating with the initial surgical team. In the case of fat grafting, complications including infection, hematoma, and injury to critical structures can occur at both the recipient and donor sites. Case: A 28-year-old female underwent large volume buttock fat grafting in the Dominican Republic and received a post-operative course of oral levofloxacin and clindamycin. After her return home, she presented to our institution on post-operative day 9 with buttock pain, tachycardia, and leukocytosis. Imaging of her buttocks showed questionable fluid collections, with no drainable abscess. Drainage of the fat grafted areas by Interventional Radiology recovered a small amount of fluid with negative initial bacterial cultures. Despite antibiotic therapy with vancomycin and meropenem and fluid resuscitation, she did not experience clinical improvement and definitive treatment required bilateral debridement of buttocks with intraoperative findings of large volume fat necrosis. Cultures from the wound grew Peptostreptococcus after a week of incubation, and the patient recovered without further surgical intervention. The case suggests that immediate post-operative antibiotics can select for slow-growing organisms, and diagnosis of large volume fat graft necrosis may be delayed given the absence of a clear abscess cavity on imaging.
Geochemical fingerprints of brannerite (UTi2O6): an integrated study
Authors: Turuani Marion , Choulet Flavien , Eglinger Aurélien , Goncalves Philippe , Machault Julie , Mercadier Julien , Seydoux-Guillaume Anne-Magali , Reynaud Stephanie , Baron Fabien , Beaufort Daniel , Batonneau Yann , Gouy Sophie , Mesbah Adel , Szenknect Stéphanie , Dacheux Nicolas , Chapon Virginie , Pagel Maurice 
Published: February 2020

AbstractBrannerite (UTi2O6) is among the major uranium-bearing minerals found in ore deposits, however as it has been long considered as a refractory mineral for leaching it is currently disregarded in ore deposits. Brannerite is found in a variety of geological environments with the most common occurrences being hydrothermal and pegmatitic. On the basis of scanning electron microscopy observations coupled with electron probe micro-analyses and laser ablation inductively-coupled plasma mass spectrometer analyses, this study describes the morphological features and the major- and trace-element abundances of brannerite samples from five hydrothermal and five pegmatitic localities across the world. Mineral compositions are also compared with observations from transmission electron microscopy and Raman spectrometry showing that brannerite is amorphous. Significant results include the definition of substitution trends and REE patterns, which are characteristics of either an occurrence or genetic type (hydrothermal and pegmatitic). Hence, in combination, it is possible to obtain reliable constraints for establishing a geochemical classification of brannerite. Inferred fingerprints have direct implications for forensic science and the exploration industry; they also contribute to a better understanding of metallogenic processes and to optimising the extraction of uranium.
Deficiency of αII-spectrin affects endothelial cell–matrix contact and migration leading to impairment of angiogenesis in vitro
Authors: Machnicka Beata , Ponceau Aurélie , Picot Julien , Colin Yves , Lecomte Marie-Christine 
Published: February 2020

Abstract
Background
Precise coordination of cytoskeletal components and dynamic control of cell adhesion and migration are required for crucial cell processes such as differentiation and morphogenesis. We investigated the potential involvement of αII-spectrin, a ubiquitous scaffolding element of the membrane skeleton, in the adhesion and angiogenesis mechanism.

Methods
The cell models were primary human umbilical vein endothelial cells (HUVECs) and a human dermal microvascular endothelial cell line (HMEC-1). After siRNA- and shRNA-mediated knockdown of αII-spectrin, we assessed its expression and that of its partners and adhesion proteins using western blotting. The phenotypes of the control and spectrin-depleted cells were examined using immunofluorescence and video microscopy. Capillary tube formation was assessed using the thick gel Matrigel matrix-based method and a microscope equipped with a thermostatic chamber and a Nikon Biostation System camera.

Results
Knockdown of αII-spectrin leads to: modified cell shape; actin cytoskeleton organization with the presence of peripheral actin patches; and decreased formation of stress fibers. Spectrin deficiency affects cell adhesion on laminin and fibronectin and cell motility. This included modification of the localization of adhesion molecules, such as αVβ3- and α5-integrins, and organization of adhesion structures, such as focal points. Deficiency of αII-spectrin can also affect the complex mechanism of in vitro capillary tube formation, as demonstrated in a model of angiogenesis. Live imaging revealed that impairment of capillary tube assembly was mainly associated with a significant decrease in cell projection length and stability. αII-spectrin depletion is also associated with significantly decreased expression of three proteins involved in capillary tube formation and assembly: VE-cadherin, MCAM and β3-integrin.

Conclusion
Our data confirm the role of αII-spectrin in the control of cell adhesion and spreading. Moreover, our findings further support the participation of αII-spectrin in capillary tube formation in vitro through control of adhesion molecules, such as integrins. This indicates a new function of αII-spectrin in angiogenesis.
Nanodetection of Head and Neck Cancer on Titanium Oxide Sensing Surface
Authors: Wang Yu , Guo Yan , Lu Jianguang , Sun Yanan , Yu Xiaoguang , Gopinath Subash C. B. , Lakshmipriya Thangavel , Wu Yuan Seng , Wang Chao 
Published: February 2020

AbstractHead and neck cancer is a heterogeneous disease, originating in the squamous cells lining the larynx (voice box), mouth, pharynx (throat), nasal cavity and salivary glands. Head and neck cancer diagnosis at the later stage is greatly influencing the survival rate of the patient. It makes a mandatory situation to identify this cancer at the earlier stages of development with a suitable biomarker. Squamous cell carcinoma antigen (SCC-Ag) is a circulating serum tumour biomarker, and the elevated level has been found in the head and neck cancer patients and highly correlated with the tumour volume. The present research was carried out to detect and quantify the level of SCC-Ag on titanium oxide (TiO2)-modified interdigitated electrode sensor (IDE) by SCC-Ag antibody. The detection of SCC-Ag was found at the level of 100 fM, while it was improved to 10 fM when the antibody was conjugated with gold nanostar, representing a 10-fold improvement. Interestingly, this enhancement in sensitivity is 1000-folds higher than other substrates. Moreover, the specificity analysis was carried out using two different control proteins and noticed that the antibody only recognised SCC-Ag, indicating the specific detection on IDE-TiO2 sensing surface.
Triptolide ameliorates fine particulate matter-induced podocytes injury via regulating NF-κB signaling pathway
Authors: Wan Qiang , Liu Zhongyong , Yang Ming , Deng Peng , Tang Nana , Liu Yanwei 
Published: February 2020

Abstract
Background
PM2.5 is associated closely with an increased risk of membranous nephropathy (MN), however, whether PM2.5 could induce podocytes injury, the underlying pathology for MN, has not be thoroughly studied. Triptolide, an active component in Tripterygium wilfordii Hook F, is frequently used to treat MN in China, but its effects on PM2.5-induced podocytes injury is still largely unknown. Therefore, we evaluated the effects of PM2.5 on podocytes, and explored whether triptolide could improve PM2.5-induced podocytes injury and the possible underlying mechanisms.

Results
Podocytes were incubated with PM2.5 after being pre-treated with triptolide, viability, apoptosis rate and migratory capacity of podocytes were determined by CCK-8 assay, flow cytometry and Transwell assay, respectively. Additionally, the levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), and superoxide dismutase (SOD) in podocytes, the cytoskeleton of podocytes, the protein expressions of nephrin, podocin, Bcl-2, Bax, nuclear factor kappa-B/p65 (NF-κB/p65) and phospho-inhibitor of NF-κB (p-IκBα) were measured. Our data showed that PM2.5 treatment significantly increased the disorganization of F-actin stress fibers, the damaged structural integrity of nucleus, the deranged and dissociated cytoskeleton in podocytes, increased the podocytes apoptosis rate, the levels of MDA and LDH, markedly up-regulated the protein expression of Bax, NF-κB/p65 and p-IκBα, down-regulated the protein expression of nephrin, podocin and Bcl-2, and significantly decreased the level of SOD, the migration rate and the viability of podocytes, compared with those of the untreated podocytes. These effects of PM2.5 on podocytes, however, were reversed by triptolide administration.

Conclusion
These results suggest that triptolide could prevent against PM2.5-induced podocytes injury via suppressing NF-κB signaling pathway.
Identification of QTL TGW12 responsible for grain weight in rice based on recombinant inbred line population crossed by wild rice (Oryza minuta) introgression line K1561 and indica rice G1025
Authors: Li Xiaoqiong , Wei Yu , Li Jun , Yang Fangwen , Chen Ying , Chen Yinhua , Guo Sibin , Sha Aihua 
Published: February 2020

AbstractBackgroundLimited genetic resource in the cultivated rice may hinder further yield improvement. Some valuable genes that contribute to rice yield may be lost or lacked in the cultivated rice. Identification of the quantitative trait locus (QTL) for yield-related traits such as thousand-grain weight (TGW) from wild rice speices is desired for rice yield improvement.ResultsIn this study, sixteenTGWQTL were identified from a recombinant inbred line (RIL) population derived from the cross between the introgression line K1561 ofOryza minutaand the rice cultivar G1025.TGW12, One of most effective QTL was mapped to the region of 204.12 kb between the marker 2,768,345 and marker 2,853,491 of the specific locus amplified fragment (SLAF). The origin ofTGW12was tested using three markers nearby or within theTGW12region, but not clarified yet. Our data indicated thirty-two open reading fragments (ORFs) were present in the region. RT-PCR analysis and sequence alignment showed that the coding domain sequences ofORF12, one MADS-box gene, in G1025 and K1561 were different due to alternative slicing, which caused premature transcription termination. The MADS-box gene was considered as a candidate ofTGW12.ConclusionThe effective QTL,TGW12, was mapped to a segment of 204.12 kb using RILs population and a MADS-box gene was identified among several candidate genes in the segment. The region ofTGW12should be further narrowed and creation of transgenic lines will reveal the gene function.TGW12could be applied for improvement of TGW in breeding program.
Genetic architecture of quantitative traits in beef cattle revealed by genome wide association studies of imputed whole genome sequence variants: II: carcass merit traits
Authors: Wang Yining , Zhang Feng , Mukiibi Robert , Chen Liuhong , Vinsky Michael , Plastow Graham , Basarab John , Stothard Paul , Li Changxi 
Published: February 2020

AbstractBackgroundGenome wide association studies (GWAS) were conducted on 7,853,211 imputed whole genome sequence variants in a population of 3354 to 3984 animals from multiple beef cattle breeds for five carcass merit traits including hot carcass weight (HCW), average backfat thickness (AFAT), rib eye area (REA), lean meat yield (LMY) and carcass marbling score (CMAR). Based on the GWAS results, genetic architectures of the carcass merit traits in beef cattle were elucidated.ResultsThe distributions of DNA variant allele substitution effects approximated a bell-shaped distribution for all the traits while the distribution of additive genetic variances explained by single DNA variants conformed to a scaled inverse chi-squared distribution to a greater extent. At a threshold ofP-value < 10–5, 51, 33, 46, 40, and 38 lead DNA variants on multiple chromosomes were significantly associated with HCW, AFAT, REA, LMY, and CMAR, respectively. In addition, lead DNA variants with potentially large pleiotropic effects on HCW, AFAT, REA, and LMY were found on chromosome 6. On average, missense variants, 3’UTR variants, 5’UTR variants, and other regulatory region variants exhibited larger allele substitution effects on the traits in comparison to other functional classes. The amounts of additive genetic variance explained per DNA variant were smaller for intergenic and intron variants on all the traits whereas synonymous variants, missense variants, 3’UTR variants, 5’UTR variants, downstream and upstream gene variants, and other regulatory region variants captured a greater amount of additive genetic variance per sequence variant for one or more carcass merit traits investigated. In total, 26 enriched cellular and molecular functions were identified with lipid metabolisms, small molecular biochemistry, and carbohydrate metabolism being the most significant for the carcass merit traits.ConclusionsThe GWAS results have shown that the carcass merit traits are controlled by a few DNA variants with large effects and many DNA variants with small effects. Nucleotide polymorphisms in regulatory, synonymous, and missense functional classes have relatively larger impacts per sequence variant on the variation of carcass merit traits. The genetic architecture as revealed by the GWAS will improve our understanding on genetic controls of carcass merit traits in beef cattle.
Long-chain acyl-CoA synthetase 2 is involved in seed oil production in Brassica napus
Authors: Ding Li-Na , Gu Shou-Lai , Zhu Fu-Ge , Ma Zhong-Yan , Li Juan , Li Ming , Wang Zheng , Tan Xiao-Li 
Published: February 2020

AbstractBackgroundTriacylglycerols (TAGs) are the main composition of plant seed oil. Long-chain acyl-coenzyme A synthetases (LACSs) catalyze the synthesis of long-chain acyl-coenzyme A, which is one of the primary substrates for TAG synthesis. InArabidopsis, the LACS gene family contains nine members, among whichLACS1andLACS9have overlapping functions in TAG biosynthesis. However, functional characterization of LACS proteins in rapeseed have been rarely reported.ResultsAn orthologue of theArabidopsis LACS2gene (BnLACS2) that is highly expressed in developing seeds was identified in rapeseed (Brassica napus). TheBnLACS2-GFP fusion protein was mainly localized to the endoplasmic reticulum, where TAG biosynthesis occurs. Interestingly, overexpression of theBnLACS2gene resulted in significantly higher oil contents in transgenic rapeseed plants compared to wild type, whileBnLACS2-RNAi transgenic rapeseed plants had decreased oil contents. Furthermore, quantitative real-time PCR expression data revealed that the expression of several genes involved in glycolysis, as well as fatty acid (FA) and lipid biosynthesis, was also affected in transgenic plants.ConclusionsA long chain acyl-CoA synthetase,BnLACS2,located in the endoplasmic reticulum was identified inB. napus. Overexpression ofBnLACS2in yeast and rapeseed could increase oil content, whileBnLACS2-RNAi transgenic rapeseed plants exhibited decreased oil content. Furthermore,BnLACS2transcription increased the expression of genes involved in glycolysis, and FA and lipid synthesis in developing seeds. These results suggested thatBnLACS2is an important factor for seed oil production inB. napus.
Characteristics and outcomes in patients with atrial fibrillation receiving direct oral anticoagulants in off-label doses
Authors: Briasoulis Alexandros , Gao Yubo , Inampudi Chakradhari , Alvarez Paulino , Asleh Rabea , Chrischilles Elizabeth , Leira Enrique C. , Vaughan-Sarrazin Mary 
Published: February 2020

Abstract
Background
We evaluated adherence to dosing criteria for patients with atrial fibrillation (AF) taking dabigatran or rivaroxaban and the impact of off-label dosing on thromboembolic and bleeding risk.

Methods
We used data for a retrospective cohort from a large U.S. health plan for Medicare beneficiaries age > =65 years with AF who initiated dabigatran or rivaroxaban during 2010–2016. Stroke and major bleeding were quantified in patients who were eligible for low dose but received standard dose, and in patients who were eligible for standard dose but received low dose.

Results
We identified 8035 and 19,712 patients who initiated dabigatran or rivaroxaban, respectively. Overall, 1401 (17.4%) and 7820 (39.7%) patients who received dabigatran and rivaroxaban met criteria for low dose, respectively. Of those, 959 (68.5%) and 3904 (49.9%) received standard dose. In contrast, 1013 (15.3%) and 2551 (21.5%) of patients eligible for standard dose dabigatran and rivaroxaban received low dose. Mean follow-up for patients eligible for low and standard dose dabigatran and rivaroxaban were 13.9, 15.1, 10.1, and 12.3 months, respectively. In unadjusted analyses, patients eligible for low or standard dose dabigatran and rivaroxaban but receiving off-label dose, had no differences in the rates of ischemic stroke. Among patients who met criteria for standard dose direct oral anticoagulants (DOAC), use of low dose was associated with significantly higher risk of any major bleeding (Dabigatran: HR = 1.44; 95% CI 1.14–1.8, P = 0.002, Rivaroxaban HR 1.34, 95% CI 1.11–1.6, P = 0.002) and gastrointestinal bleeding (Dabigatran: HR = 1.48; 95% CI 1.08–2, P = 0.016). In patients who met criteria for low dose DOACs, there was lower risk of major bleeding (Dabigatran: HR = 0.59; 95% CI 0.43–0.8, P < 0.001), gastrointestinal (Rivaroxaban: HR 0.79; 95% CI 0.64–0.98, P = 0.03) and intracranial bleeding (Dabigatran: HR = 0.33; 95% CI 0.12–0.9, P = 0.001) with standard dosing. After propensity matching, use of off-label doses was not associated with stroke, major, gastrointestinal or intracranial bleeding for either dabigatran or rivaroxaban.

Conclusions
While a significant number of patients receive higher or lower dose of dabigatran and rivaroxaban than recommended, we found no evidence of significant impact on thromboembolic or hemorrhagic outcomes.
Intake of dairy protein during pregnancy in IBD and risk of SGA in a Norwegian population-based mother and child cohort
Authors: Bengtson May-Bente , Haugen Margaretha , Brantsæter Anne Lise , Aamodt Geir , Vatn Morten H. 
Published: February 2020

Abstract
Background
Patients with inflammatory bowel disease (IBD) tend to avoid dairy products to minimize abdominal pain and diarrhea. The aim of this study was to estimate the proportion of protein from dairy sources (PPDS) in mothers with and without IBD, and to explore the impact of PPDS on inadequate gestational weight gain (GWG) or small for gestational age (SGA) in IBD compared to non-IBD in the population-based Norwegian Mother, Father and Child Cohort Study (MoBa).

Methods
MoBa includes about 95,000 pregnant women recruited throughout Norway from 1999 to 2008. IBD phenotype and complications during pregnancy and delivery were ascertained. This study included 148 mothers with Crohn disease (CD) and 194 with ulcerative colitis and 68,858 non-IBD mothers. In mid-pregnancy participants answered a comprehensive semi-quantitative food frequency questionnaire assessing diet since the start of pregnancy. PPDS was ranked in quartiles. The two lowest quartiles were merged and considered to represent the lowest of three PPDS groups. We used logistic regression analyses to model multivariate associations, adjusting for potential confounders.

Results
The risk of belonging to the lowest PPDS group was twice as high in IBD mothers compared to non-IBD mothers (aOR = 2.02, 95% CI: 1.53, 2.67). Low compared to high PPDS strongly predicted inadequate GWG in CD (aOR = 4.22, 95% CI: 1.28, 13.92). Surprisingly, and in opposition to the non-IBD mothers, PPDS was positively associated with the risk of SGA in IBD mothers. IBD mother with low PPDS was associated with significantly lower risk of SGA than non-IBD mothers and IBD mothers with high PPDS (aOR = 0.19, 95% CI: 0.07, 0.50). The interaction term IBD/PPDS was the factor that linked SGA to IBD compared to non-IBD, and increased the association between IBD and SGA with a factor of three.

Conclusion
This study shows that intake of dairy products is lower in IBD mothers than in non-IBD mothers, and further, that low intake of dairy products in IBD mothers is associated with reduced risk of SGA compared to non-IBD and IBD mothers with high PPDS.
Mobile device use and the cognitive function and depressive symptoms of older adults living in residential care homes
Authors: Lin Lu , Jing Xiu-Chen , Lv Shu-Jiao , Liang Jing-Hong , Tian Li , Li Hui-Ling , Puts Martine , Xu Yong 
Published: February 2020

Abstract
Background
With the fast-paced aging and increasing digitalization of society, there has been a growing interest in the effect of mobile device use on cognitive function and depression in older adults. However, research examining this issue among older adults in residential care homes (RCHs) is scant. Therefore, this study aimed to examine the impact of mobile device use on the cognitive function and depressive symptoms of older adults living in RCHs.

Methods
A cross-sectional survey was conducted using a sociodemographic questionnaire, the Montreal Cognitive Assessment (MoCA) and the 15-item Geriatric Depression Scale (GDS-15).

Results
A total of 235 senior residents (aged 82.58 ± 5.54) in four RCHs were surveyed. Users of mobile devices had a significantly higher total MoCA score (25.02 ± 4.14) and a significantly lower GDS-15 score (3.28 ± 2.74) than non-users (MoCA: 19.34 ± 5.21, GDS-15: 4.69 ± 2.90). Multivariate linear regression indicate that mobile device use is significantly associated with total MoCA score, six of the seven sub-scores (visuospatial abilities and execution functions, attention, language, abstraction, delayed recall, and orientation)(P < 0.05). Logistic regression showed that mobile device use was significantly associated with the level of depressive symptoms (OR = 0.458, 95%CI = 0.249–0.845).

Conclusions
Use of mobile devices has a significant association with the cognitive function and depressive symptoms of older adults living in RCHs, and thus should be encouraged as a measure to maintain and improve cognition and prevent depression.