bluuebunny/crossref_metadata_2025_split huggingface dataset:
SUCCESSION PROCESS IN THE FAMILY BUSINESS: A CASE STUDY OF PEKALONGANESE BATIK CLOTH ENTERPRISES, INDONESIA
Authors: Kusumantoro  , Suman Agus , Umi Mintarti Widjaja Sri , Wahyono Hari 
Published: March 2020

Purpose: The main purpose of this study is to explain the role of parents in teaching business management to their children from an early age for the success of family business succession.
Methodology: It was a qualitative study and data were collected by observation, in-depth interviews, and documentation. The focus of this study was the succession process of the PekalonganBatik business analyzed from the entrepreneurial learning perspective. Interview questions focused on entrepreneurial learning and how parents teaching business management. Interviews conducted into 6 Informants. Each interview took between 35 – 45 minutes.
Main Finding: The results of this study showed that parents usually introduced business management to their children from an early age. They made their children involved in the business to know about the planning, producing and marketing of Batik cloths. The indicators of giving entrepreneurial learning to children are involving them in business activities; they learned to manage the business by doing those activities at their parents’business.
Implications/Applications: This study can be useful for another batik enterprises center to guidelines for succession.
Novelty/Originality: This research was to explore the parents to involve their children from an early age to help manage businesses in preparation for business succession.
Measuring Fertility Health Knowledge in University Students: Development and Testing of a Survey Tool
Authors: Barron Mary Lee , Lithgow Diana , Wade Gail , Mueller-Luckey Georgia 
Published: March 2020

Background and PurposeFew surveys address fertility health in both men and women. This report details development of a fertility knowledge assessment tool for clinical and research settings that would sensitize young adults to habits that would promote healthy fertility.MethodsA pilot study (N = 31) followed by a larger study of students at three schools (N = 465, 335, & 375) at a midwestern university using a new 30-item survey.ResultsFace and content validity of the survey tool were determined by a panel of experts. Internal consistency and reliability were acceptable for a new instrument (α = .81 for the total group; α = .80 for each school.ConclusionsThis instrument is a valid and reliable short screening tool that can be used to assess knowledge of fertility and possibly open discussions about fertility self-care.
The Minimal Eating Observation Form–II Danish Version: Psychometric and Metrological Aspects
Authors: Westergren Albert , Melgaard Dorte 
Published: March 2020

Background and purposeThe Minimal Eating Observation Form – II (MEOF-II) is a screening and research tool for eating difficulties. We assess psychometric and metrological aspects of the Danish version of the tool.MethodsThis study includes MEOF-II data from 302 acute geriatric patients. Analyses were based on Classical Test Theory and Rasch Model Analysis.Results51.3% of the patients had eating difficulties. The MEOF-II items belonged to one higher order factor, and three lower level factors: deglutition; ingestion; and energy/appetite. The hierarchical structure of the items was revealed. The MEOF-II had a good fit with Rasch model expectations.ConclusionThe study provides support for the reliability and validity of the Danish version of the MEOF-II. MEOF-II total and subscale scores are reliable and valid for use in nursing practice and research.
The Effect of Vitamin C Addition on Epigallocatechin Gallate (EGCG) Stability in Green Tea Solution
Authors: Rahman Alief Putriana , Purwanto Djoko Agus , Isnaeni Isnaeni 
Published: March 2020

Background: Epigallocatechin gallate (EGCG) is the most abundant green tea catechin with a powerful antioxidant effect to prevent cancer cells. EGCG in green tea solution is highly susceptible to degradation, this it is urgent to increase the stability of EGCG by vitamin C addition. Vitamin C can regenerate radical EGCG to be normal EGCG. Objective: This research aim was to determine percent of decreased level of EGCG before and after vitamin C addition. Methods: This paper was focused on enhancing the stability of EGCG by 1 mg (GTVC1), 1.5 mg (GTVC2), 2 mg (GTVC3), 2.5 mg (GTVC4) and 3 mg (GTVC5) of vitamin C addition to 10 g/L of green tea solution concentration. Evaluations of EGCG were conducted at 0 days, 1 day, 2 days, 3 days, and 4 days of storage time. EGCG was analyzed using thin layer chromatography (TLC) densitometry methods. The stability of EGCG was determined by % of decreased EGCG. Results: Percent of loss of EGCG in GT, GTVC1, GTVC2, GTVC3, GTVC4 and GTVC5 after 4 days storage were 19.93%, 10.89%, 21.08%, 18.18%, 28.56%, and 9.76%, respectively. Conclusion: This study showed that in 4 days storage, the decreased level of EGCG in green tea solution with 3 mg of vitamin C addition (GTVC5) was 9.76% which was smaller than green tea solution without vitamin C addition (GT) which EGCG decreasing 19.93%.
Fibrous Cleanout Fluids in Horizontal and Inclined Wells
Authors: Elgaddafi Rida , Ahmed Ramadan 
Published: March 2020

Abstract
This paper presents the results of experimental and theoretical studies conducted to investigate the hole cleaning performance of fibrous fluids in horizontal and inclined wells. Coiled tubing is commonly applied in the wellbore cleanout operations of highly deviated and horizontal wells. Accumulation of proppant and other solid debris in the wellbore remarkably impedes field operations and oil and gas production. Fibrous fluids have shown great potential for cleaning solids that are difficult to remove with conventional fluid systems. The addition of fiber substantially reduces the settling velocity of particles in fluids due to the formation of a fiber network. This study is aimed to investigate wellbore cleanout and hydraulics of fibrous fracturing fluid in horizontal and inclined wells.
Cleanout experiments were carried out using Xanthan gum suspensions with and without fiber (i.e. monoﬁlament synthetic ﬁber). Extensive flow loop tests were performed varying fiber concentration and inclination while measuring the equilibrium bed height at different flow rates. A 22-ft long annular test section was utilized to perform the cleanout (erosion) experiments. To better explain the results, rheological and hydraulic characteristics of the test fluids were examined using the pipe viscometer and the annular test section. A model has been developed to calculate the critical velocity and flow rate needed to initiate the movement of bed particles during hole cleaning operation. The model is formulated to account for the presence of fiber through a fiber drag coefficient.
Results show a noticeable reduction in critical velocity or equilibrium bed height owing to the addition of a small amount (0.04% by wt.) of fiber. Fibrous fluid demonstrated better cleanout performance than the non-fibrous fluid (base fluid). The addition of fiber to the base fluid, significantly enhanced hole cleanout, resulting in a drastic reduction of equilibrium bed height. At 70 gpm, fibrous fluid cleaned the test section 50% better than the base fluid. One possible explanation for this observation could be the development of a fiber network in the fluid that provides additional drag to agitate bed particles and initiate their movement. Results of rheology and hydraulic tests show that the addition of fiber (0 to 0.08%) had very little effect on the rheology and hydraulics of fibrous fluid. Fibrous fluid displayed slightly lower pressure loss as compared to the base fluid, which indicates minor friction reduction due to the fiber.
The accuracy of the hydraulic model developed in the study has been assessed using flow loop measurements. Results show good agreement between predictions and measurements. The new model can be employed in the field to optimize the wellbore cleanout operation with fibrous fluid without increasing bottom hole pressure.
Psychosocial Syndemic of Suicidal Ideation: A Cross-sectional Study among Sexually Transmitted Infection Patients in Shanghai, China
Authors: Wang Suping , Ni Yang , Gong Ruijie , Shi Yuan , Cai Yong , Ma Jin 
Published: March 2020

Abstract

Background: We sought to verify whether psychosocial health problems among patients with sexually transmitted infections (STIs) were associated with these patients’ suicidal ideation and to examine the syndemic effect of multiple psychosocial problems on suicidal ideation. Methods: This was a cross-sectional study of 519 STI patients at the Shanghai Skin Disease Hospital. Demographic, psychosocial, and suicidal ideation information about the participants was collected by questionnaire. Logistic regressions were performed to detect the association between demographic variables and suicidal ideation, as well as each individual psychosocial variable and suicidal ideation, and to verify the syndemic effect of psychosocial factors. Results: Of the participants, 25.0% (130/519) reported having suicidal ideation. In univariable analysis, low self-esteem, loneliness, depression, entrapment, defeat, and unsatisfied interpersonal needs were associated with suicidal ideation. Multivariable analysis found depression (odds ratio [OR]: 4.1; 95% confidence interval [CI]: 2.3–7.2) and entrapment (OR: 2.1; 95%CI: 1.1–4.1) each had a more significant relation with suicidal ideation than the other psychosocial problems examined. STI patients who experienced two or more psychosocial health problems had approximately fourfold odds of suicide ideation (adjusted OR [AOR]: 4.2; 95%CI: 2.6–6.8) compared with those in the non-syndemic group, especially in the high-level (five or more psychosocial problems) group (AOR: 7.0; 95%CI: 3.9–12.5). Conclusions: The study found the participants had a high rate of suicidal ideation and suffered from severe psychosocial problems. These results show a syndemic effect of psychosocial problems on increasing the odds of suicidal ideation. Our findings suggest an urgent need for efforts to prevent suicidal ideation among STI patients toward improving the social and health conditions of this population.
360-Degree Delphi: addressing sociotechnical challenges of healthcare IT
Authors: Waldmueller Heiko Dominik , Spreckelsen Cord , Rudat Hannah , Krumm Norbert , Rolke Roman , Jonas Stephan Michael 
Published: March 2020

Abstract
This approach uses the strong advantages a Delhi study has, identifies the disadvantages of traditional Delphi techniques and aims to introduce and evaluate a modified approach called 360-Degree Delphi. Key aspects of 360-Degree Delphi are tested by applying the approach to the needs and requirements analysis of a system for managing patients’ advance directives and living wills. 360-Degree Delphi, as a modified Delphi process, is specified as a structured workflow with the optional use of stakeholder groups. Consensus is created within individual stakeholder groups, but is also communicated between groups, while the iterative structure of the Delphi process remains unchanged. We hypothesize that (1) 360-Degree Delphi yields complementary statements from different stakeholders, which would be lost in classical Delphi; while (2) the variation of statements within individual stakeholder groups is lower than within the total collective. A user study is performed that addresses five stakeholder groups (patients, relatives, medical doctors, nurses and software developers) on the topic of living will communication in an emergency context. About 25% of the invited experts (stakeholders) agreed to take part in the Delphi round 0 (three patients, two relatives, three medical doctors, two qualified nurses and three developers), forming a structured panel of the five stakeholder groups. Two raters created a bottom-up coding, and 238 thematic codes were identified by the qualitative text analysis. Based on a consented coding list, a quantitative online-questionnaire was developed and send to different stakeholder groups. With respect to the hypotheses, Delphi round 0 had the following results: (1) doctors had a completely different focus from all the other stakeholder groups on possible channels of communications with the patient; (2) the dispersion of codes within individual stakeholder groups and within the total collective was approximately 28% higher in the total collective than in the sub-collectives, but without a marked effect size. With respect to the hypotheses, Delphi round 1 had the following results: different stakeholder groups had highly diverging opinions with respect to central questions on IT-development. In comparison, the total group would not be representative of either of these individual stakeholder needs (mean 4.344, stdev +/- 1.870)
Gene expression profiling identifies pathways involved in seed maturation of Jatropha curcas
Authors: Maghuly Fatemeh , Deak Tamas , Vierlinger Klemens , Pabinger Stephan , Tafer Hakim , Laimer Margit 
Published: March 2020

Abstract
Background: Jatropha curcas, a tropical shrub, is a promising biofuel crop, which produces seeds with high content of oil and protein. To better understand the maturation process of J. curcas seeds and to improve its agronomic performance, a two-step approach was performed in six different maturation stages of seeds: 1) generation of the entire transcriptome of J. curcas seeds using 454-Roche sequencing of a cDNA library, 2) comparison of transcriptional expression levels using a custom Agilent 8x60K oligonucleotide microarray.
Results: A total of 793,875 high-quality reads were assembled into 19,382 unique full-length contigs, of which 13,507 could be annotated with Gene Ontology (GO) terms. Microarray data analysis identified 9,111 probes (out of 57,842 probes), which were differentially expressed between the six maturation stages. The expression results were validated for 75 selected transcripts based on expression levels, predicted function, pathway, and length.
Result from cluster analyses showed that transcripts associated with fatty acid, flavonoid, and phenylpropanoid biosynthesis were over-represented in the early stages, while those of lipid storage were over-represented in the late stages. Expression analyses of different maturation stages of J. curcas seed showed that most changes in transcript abundance occurred between the two last stages, suggesting that the timing of metabolic pathways during seed maturation in J. curcas occurs in late stages. The co-expression results showed that the hubs (CB5-D, CDR1, TT8, DFR, HVA22) with the highest number of edges, associated with fatty acid and flavonoid biosynthesis, are showing a decrease in their expression during seed maturation. Furthermore, seed development and hormone pathways are significantly well connected.
Conclusion: The obtained results revealed differentially expressed sequences (DESs) regulating important pathways related to seed maturation, which could contribute to the understanding of the complex regulatory network during seed maturation with the focus on lipid, flavonoid and phenylpropanoid biosynthesis. This study provides detailed information on transcriptional changes during J. curcas seed maturation and provides a starting point for a genomic survey of seed quality traits. The results highlighted specific genes and processes relevant to the molecular mechanisms involved in Jatropha seed maturation. These data can also be utilized regarding other Euphorbiaceae species.
How can we support best practice? A situational assessment of injury prevention practice in public health.
Authors: Richmond Sarah A. , Carsley Sarah , Prowse Rachel , Manson Heather , Moloughney Brent 
Published: March 2020

Abstract
Background: To effectively impact the significant population burden of injury, we completed a situational assessment of injury prevention practice within a provincial public health system to identify system-wide priorities for capacity-building to advance injury prevention in public health. Methods: A descriptive qualitative study was used to collect data on the current practice, challenges and needs of support for injury prevention. Data was collected through semi-structured interviews (n=20) and focus groups (n=19). Participants included a cross-section of injury prevention practitioners and leadership from public health units reflecting different population sizes and geographic characteristics, in addition to public health researchers and experts from academia, public health and not-for-profit organizations. Thematic analysis was used to code all of the data by one reviewer, followed by a second independent reviewer who coded a random selection of interview notes. Major codes and sub codes were identified and final themes were decided through iterations of coding comparisons and categorization. Once data were analysed, we confirmed the findings with the field, in addition to participating in a prioritization exercise to surface the top three needs for support. Results: Major themes that were identified from the data included: current public health practice challenges; capacity and resource constraints, and; injury as a low priority area. Overall, injury prevention is a broad, complex topic that competes with other areas of public health. Best practices are challenged by system-wide factors related to resources, direction, coordination, collaboration, and emerging injury public health issues. Injury is a reportedly under prioritized and under resourced public health area of practice. Practitioners believe that increasing access to data and evidence, and improving collaboration and networking is required to promote best practice. Conclusions: The results of this study suggest that there are several system level needs to support best practice in public health injury prevention in Ontario including reducing research to practice gaps and supporting opportunities for collaboration. Our research contributes to the literature of the complexity of public health practice, and presents several mechanisms of support to increase capacity at a system level to improve injury prevention practice, and eventually lessen the population burden of injury.
Virtual Reality Video Promotes Effectiveness in Advance Care Planning
Authors: Hsieh Wan-Ting 
Published: March 2020

Abstract
Background: In 2019, the Patient Autonomy Act went into effect, allowing Taiwanese citizens to establish legal advance decisions. In an effort to secure a more realistic and accurate perception of situations, a virtual reality video was developed by the palliative care team of Chi-Mei hospital in southern Taiwan for citizens to use before advance care planning. This study explores the change in participants’ preference and certainty regarding end-of-life decisions after using this tool.
Methods: Participants were at least 20 years old and capable of reading and understanding the information provided in the written handout with information about the legal process of making an advance decision. They completed pre-test questionnaires, viewed a six-minute 360-degree virtual reality video on a portable headset and then completed a post-test questionnaire about their preference on the 5 medical options including CPR, life-sustaining treatments, antibiotics, blood transfusion, and artificial nutrition and hydration, followed by feedback on the helpfulness of the virtual reality. The control group included 40 participants who only read the handout and they also completed pre-test and post-test questionnaires.
Results: After viewing the virtual reality video, preference for refusing CPR, life-sustaining treatment, antibiotics, blood transfusion, and artificial nutrition and hydration increased significantly in the virtual reality intervention group. Uncertainty regarding the 5 medical options mentioned above are significantly decreasing. The intervention was generally recognized by participants for its help of making decision.
Discussion: The decrease in the number of participants who could not make decisions indicate that the virtual reality video may be helpful for users in making end-of-life decision. According to feedback, the virtual reality video helped equip users with better understanding of medical scenarios, and it is a good decision aid for advance care planning.
Conclusion: This is the first study since the Patient Autonomy Act has been passed that explores the effectiveness of using a virtual reality video as a decision aid in advance care planning and reveals decreased preference of CPR, life sustaining treatment, antibiotics, blood transfusion and artificial nutrition and hydration after intervention. This decision aid proved to be an effective tool for clarifying their end-of-life care preferences.
DNMT1 inhibits hepatocellular carcinoma cells by altering the epigenetic modification of Marveld1 gene
Authors: Zhang Song Yan , Luo Qian , Wang Peng , Zhao Hao Ran , Xu Hai Tao , Zhao Liang , Qu Zhao Wei , Sun Jian Min , Zhang Yu Bao , Zheng Biao 
Published: March 2020

Abstract
Objective

To study the effect of DNA methyltransferase (DNMT1) on Marveld1 in hepatocellular Carcinoma (HCC), and explore its role in the molecular mechanism of HCC occurrence and development.

Methods

The expression level of Dnmt1 and Marveld1 genes was detected by RT-PCR in the intraoperative pathological specimens. The human hepatoma Bel7402 and SMMC7721 cell lines down-regulated by DNMT1 gene expression were constructed by SiRNA transfection. The methylation level of Marveld1 promoter region was detected after RT-PCR and West-blot verification. The expression of Marveld1 gene, cell proliferation, invasion and change of cell cycle were detected by RT-PCR and West-blot. The expressions of P53, CyclinD1, P21 and P16 protein were detected by West-blot method.

Results

The expression of DNMT1 gene in human hepatocarcinoma tissues was higher than that in adjacent tissues. The methylation level of the Marveld1 gene promoter region in human hepatoma cell line down-regulated by DNMT1 gene expression was decreased, the expression level of Marveld1 gene was increased, and the proliferation and invasion ability of cells were weakened. The cell cycle was showed as G1-S phase arrest; P53 and P16 protein expressions were up-regulated, and CyclinD1 and P21 protein expressions were down-regulated.

Conclusions

Dnmt1 gene is highly expressed in human hepatocarcinoma tissues. Down-regulation of DNMT1 gene expression can decrease the methylation level of Marveld1 promoter region, up-regulate Marveld1 gene and human P53 and P16 proteins expressions, and down-regulate CyclinD1 and P21 proteins expressions, inhibit the proliferation and invasion of human hepatoma cells and can cause cell cycle G1-S arrest.