bluuebunny/crossref_metadata_2025_split huggingface dataset:
The relationship between vitamin D deficiency and oxidative stress can be independent of age and gender
Authors: Câmara Alice Barros , Brandão Igor Augusto 
Published: November 2019

Abstract. The active vitamin D (1,25(OH)2D) acts through a nuclear receptor to perform several functions in cellular metabolism. 1,25(OH)2D participates directly in calcium homeostasis, regulates the immune system, nervous system, blood pressure, insulin secretion, among others. Vitamin D deficiency could also be associated with several diseases and increased cellular oxidative damage. The present study aimed to investigate whether lipid peroxidation and/or protein oxidation are affected by vitamin D deficiency and whether sunlight exposure/diet, gender, and age might influence this relationship. Vitamin D concentrations were obtained from the Heart Hospital database and a questionnaire was applied among the 212 participants. We used the inactive vitamin D (25(OH)2) in the analyses since 1,25(OH)2D has a short half-life and a low blood concentration. Lipid peroxidation and protein oxidation analyses were performed using spectrophotometry. Multivariate analyses suggested the participation of vitamin D deficiency (<30 ng/mL) and sunlight/diet in oxidative stress (p <0.05; R2 MDA: 0.562; R2 CG: 0.429). Multiple linear regression test show that the age and gender of patients are not interfering in the analyses (p>0.05). Therefore, we suggest that the relationship between vitamin D deficiency and oxidative stress can be independent of age and gender.
Regional Modeling of Antarctic Bottom Water Flows in the Key Passages of the Atlantic
Authors: Frey D. I. , Morozov E. G. , Fomin V. V. , Diansky N. A. , Tarakanov R. Y. 
Published: November 2019

AbstractThe goal of this research is to modify and apply a version of high‐resolution three‐dimensional numerical model for simulations of bottom circulation and to study the flows of Antarctic Bottom Water in abyssal channels of the Atlantic Ocean using this model. We adjusted the Institute of Numerical Mathematics Ocean Model σ‐level ocean circulation model for several regions with intense bottom currents in abyssal channels. High vertical resolution near the seafloor allowed us to study the abyssal part of the ocean circulation, while high horizontal resolution is necessary for modeling currents in narrow underwater channels and fracture zones. We used our direct velocity measurements carried out at key points of the currents in the channels for verification of the model. This approach was applied in the regions with different seafloor topography: in the long and narrow Vema Channel with a strong bottom current and in several fracture zones of the Mid‐Atlantic Ridge with rough bathymetry. On the basis of simulated three‐dimensional velocity fields, we analyzed the spatial structure of the bottom currents along the entire length of the channels, determined maximum velocities at different sections, investigated the influence of the Ekman flux on the structure of the flows, and compared our model results with in situ observations. We also calculated the total transports of Antarctic Bottom Water through the fractures in several underwater ridges of the Atlantic Ocean.
New Insight Into the Formation and Evolution of the East Reykjanes Ridge Current and Irminger Current
Authors: Petit T. , Mercier H. , Thierry V. 
Published: November 2019

AbstractThe Reykjanes Ridge strongly influences the circulation of the North Atlantic Subpolar Gyre as it flows to the Irminger Sea from the Iceland Basin. The circulation is composed of two main along‐ridge currents: the southwestward East Reykjanes Ridge Current (ERRC) in the Iceland Basin and the northeastward Irminger Current (IC) in the Irminger Sea. To study their interconnection through the ridge, as well as their connections with the interior of each basin, velocity and hydrological measurements were carried out along and perpendicular to the crest of the Reykjanes Ridge in June–July 2015 as part of the Reykjanes Ridge Experiment project. This new data set changes our view of the ERRC and IC as it reveals undocumented along‐stream evolutions of their hydrological properties, structures, and transports. These evolutions are due to flows connecting the ERRC and IC branches at specific locations set by the bathymetry of the ridge and to significant connections with the interiors of the basins. Overall, the ERRC transport increases by 3.2 Sv between 63°N and 59.5°N and remains almost constantly southward. In the Irminger Sea, the increase in IC transport of 13.7 Sv between 56°N and 59.5°N, and the evolution of its properties are explained by both cross‐ridge flows and inflows from the Irminger Sea. Further north, bathymetry steers the IC northwestward into the Irminger Sea. At 63°N, the IC water masses are mostly issued from the cross‐ridge flow.
Modeling the Dispersion of Dissolved Natural Gas Condensates From the Sanchi Incident
Authors: Chen Lei , Yang Jiayan , Wu Lixin 
Published: November 2019

AbstractAn Iranian tanker with 136,000 tons of natural gas condensates collided with a freighter in the East China Sea in January 2018 and, after drifting ablaze for 8 days and over 200 km, capsized on the edge of the shelf near the Kuroshio Current. Different from the crude oil, the condensates consist of hydrocarbons that have relatively high solubility in seawater. We postulate that the leakage from the remaining condensate cargo at 110 m depth may result in a bottom layer of condensate‐enriched water in the vicinity of the resting tanker. A model is constructed in this study to simulate the dispersion of contaminated water through the processes of oceanic advection, diffusion, biodegradation, and volatilization. It is found that the scope and magnitude of the dispersion are most sensitive to the biodegradation. Even though the biodegradation time scale depends on several factors that are not well quantified in this region, using any value within the estimated range from a previous study results in significant contamination in the broad area. The dispersion is particularly effective in this incident because the tanker capsized near the Kuroshio Current—a fast‐moving western boundary current. The Kuroshio acts as a fast conduit to spread the contaminant to the east coast of Japan and the interior Pacific Ocean. In addition, we identify that the Tsushima Warm Current, a perennial flow into the Japan Sea, is the second major conduit for spreading the polluted water. This study indicates that dissolved hydrocarbons are the main environmental risk for maritime spills of natural gas condensates.
Mars Water and D/H Evolution From 3.3 Ga to Present
Authors: Alsaeed N. R. , Jakosky B. M. 
Published: November 2019

AbstractThe current deuterium to hydrogen ratio (D/H) on Mars is enriched by a factor of 5–6 relative to terrestrial values, suggesting that large amounts of H from water have been lost to space. Loss of H occurs more efficiently than loss of D because H atoms are lighter than D atoms, so the remaining gas becomes enriched in D. We constrain the history of water on Mars using D/H by tracking the supply and loss of H and D in the atmosphere. We examined the evolution of water and D/H from 3.3 Ga to the present, using the measured D/H in an ~3‐billion‐year‐old Gale crater mudstone and in the present atmosphere as constraints. We define the boundary conditions by the amount of water present at the surface early in history and the amount of water present today and incorporate the supply of water from outgassing and loss of H and D to space. The factor‐of‐2 enrichment in D/H in the last 3.3 Ga can be produced if loss to space outstrips outgassing. This corresponds to a present‐day 20‐ to 50‐m water global equivalent layer (GEL) that is a residual of an initial inventory at 3.3 Ga of 40‐ to 170‐m GEL, combined with 5‐ to 100‐m GEL outgassed and 20‐ to 220‐m GEL lost to space.
Northern Hemisphere Greening in Association With Warming Permafrost
Authors: Peng Xiaoqing , Zhang Tingjun , Frauenfeld Oliver W. , Wang Shijin , Qiao Lina , Du Ran , Mu Cuicui 
Published: November 2019

AbstractVegetation is closely tied to climate change, hydrological processes, the carbon cycle, and the energy balance. However, in cold regions, both climate and vegetation changes are also closely coupled to permafrost. The association between amplified warming and greening in Northern Hemisphere permafrost regions is not clearly understood. We therefore produce an extended 1982–2015 normalized difference vegetation index record based on concatenated Global Inventory Modeling and Mapping Studies and Moderate‐Resolution Imaging Spectroradiometer data to quantify the spatiotemporal patterns of vegetation variability. We also establish spatiotemporal permafrost warming patterns based on a thawing index, active layer thickness, soil temperature, the first thaw date, and thawing days. Next, we establish the association between vegetation and warming permafrost. We find that normalized difference vegetation index increases in approximately 70% of Northern Hemisphere permafrost regions during the growing season (April–October) and in 72% of the region during autumn. Warming permafrost shows a positive relationship with greening. A higher thawing index, greater active layer thickness, higher soil temperature, and also increased precipitation are linked with the observed greening. An earlier first thaw date and an increased number of thawing days also correlate with vegetation greening. These findings underscore the sensitivity of vegetation to warming permafrost. Continued permafrost warming will result in further greening of Northern Hemisphere cold regions (and vice versa), with important implications for the permafrost soil‐vegetation‐atmosphere carbon cycles, and the water, nutrient, and energy budgets.
Seasonal and Latitudinal Response of New Zealand Sea Surface Temperature to Warming Climate Since the Last Glaciation: Comparing Alkenones to Mg/Ca Foraminiferal Reconstructions
Authors: Sikes Elisabeth L. , Schiraldi Benedetto , Williams Amanda 
Published: November 2019

AbstractDual proxies were used to estimate paleo sea surface temperatures (SST) for the Bay of Plenty, north of New Zealand.
and Mg/Ca in the planktonic foraminifera Globogerina bulloides reconstruct SST for the growth seasons for the organisms they are based upon.
SST (summer) were consistently ~ 3.5 °C warmer than Mg/Ca (spring), suggesting that Bay of Plenty SST during the last glacial maximum (LGM) was 17.3 °C in summer and 13.8 °C in spring. Combining these results with published data based on the same proxies from other sites around New Zealand shows cooling of 3–4 °C in both seasons at all sites in the LGM relative to the Holocene. This indicates that overall, glacial surface water cooling was similar in subtropical and subpolar waters in both spring and summer. This contrasts with published foraminiferal assemblage reconstructions suggesting greater subantarctic cooling during the LGM. Deglacial warming across the region was characterized by changes in both seasonal and latitudinal temperature differences. Warming began in subtropical waters at ~21 ka, ~ 1.5 ka earlier than in subantarctic water. In the Bay of Plenty, the seasons maintained a consistent offset, while in Hawke Bay, springs stayed cold while summers warmed until after the Antarctic Cold Reversal. In contrast, subantarctic spring SST warmed rapidly, causing temperature differences to decrease between the Chatham Rise (subantarctic) and subtropical sites, possibly caused by shifting westerly winds. The use of multiple proxies enhances our understanding by adding a seasonal component to the glacial story of climate change in the southwest Pacific Ocean.
Deep Crustal Flow Within Postorogenic Metamorphic Core Complexes: Insights From the Southern Western Gneiss Region of Norway
Authors: Wiest J. D. , Osmundsen P. T. , Jacobs J. , Fossen H. 
Published: November 2019

AbstractViscous crustal flow can exhume once deeply buried rocks in postorogenic metamorphic core complexes (MCCs). While migmatite domes record the flow dynamics of anatectic crust, the mechanics and kinematics of solid‐state flow in the deep crust are poorly constrained. To address this issue, we studied a deeply eroded and particularly well‐exposed MCC in the southern Western Gneiss Region of Norway. The Gulen MCC formed during Devonian transtensional collapse of the Caledonian orogeny in the footwall of the Nordfjord‐Sogn detachment zone. We developed a semiquantitative mapping scheme for ductile strain to constrain micro‐ to megascale processes, which brought eclogite‐bearing crust from the orogenic root into direct contact with Devonian supradetachment basins. The Gulen MCC comprises different structural levels with distinct metamorphic evolutions. In the high‐grade core, amphibolite‐facies structures record fluid‐controlled eclogite retrogression and coaxial flow involving vast extension‐perpendicular shortening. Detachment mylonites formed during ductile‐to‐brittle noncoaxial deformation and wrap around the core. We present a sequential 3‐D reconstruction of MCC formation. In the detachment zone, the combined effects of simple shearing, incision/excision, and erosion thinned the upper crust. Internal necking of the ductile crust was compensated by extension‐perpendicular shortening within the deep crust and resulted in differential folding of distinct crustal levels. We identify this differential folding as the main mechanism that can redistribute material within solid‐state MCCs. Our interpretation suggests a continuum of processes from migmatite‐cored to solid‐state MCCs and has implications for postorogenic exhumation of (ultra‐)high‐pressure rocks.
Alterations in ZnT1 expression and function lead to impaired intracellular zinc homeostasis in cancer
Authors: Lehvy Adrian Israel , Horev Guy , Golan Yarden , Glaser Fabian , Shammai Yael , Assaraf Yehuda Gérard 
Published: November 2019

AbstractZinc is vital for the structure and function of ~3000 human proteins and hence plays key physiological roles. Consequently, impaired zinc homeostasis is associated with various human diseases including cancer. Intracellular zinc levels are tightly regulated by two families of zinc transporters: ZIPs and ZnTs; ZIPs import zinc into the cytosol from the extracellular milieu, or from the lumen of organelles into the cytoplasm. In contrast, the vast majority of ZnTs compartmentalize zinc within organelles, whereas the ubiquitously expressed ZnT1 is the sole zinc exporter. Herein, we explored the hypothesis that qualitative and quantitative alterations in ZnT1 activity impair cellular zinc homeostasis in cancer. Towards this end, we first used bioinformatics to analyze inactivating mutations in ZIPs and ZNTs, catalogued in the COSMIC and gnomAD databases, representing tumor specimens and healthy population controls, respectively. ZnT1, ZnT10, ZIP8, and ZIP10 showed extremely high rates of loss of function mutations in cancer as compared to healthy controls. Analysis of the putative functional impact of missense mutations in ZnT1-ZnT10 and ZIP1-ZIP14, using homologous protein alignment and structural predictions, revealed that ZnT1 displays a markedly increased frequency of predicted functionally deleterious mutations in malignant tumors, as compared to a healthy population. Furthermore, examination of ZnT1 expression in 30 cancer types in the TCGA database revealed five tumor types with significant ZnT1 overexpression, which predicted dismal prognosis for cancer patient survival. Novel functional zinc transport assays, which allowed for the indirect measurement of cytosolic zinc levels, established that wild type ZnT1 overexpression results in low intracellular zinc levels. In contrast, overexpression of predicted deleterious ZnT1 missense mutations did not reduce intracellular zinc levels, validating eight missense mutations as loss of function (LoF) mutations. Thus, alterations in ZnT1 expression and LoF mutations in ZnT1 provide a molecular mechanism for impaired zinc homeostasis in cancer formation and/or progression.
Ultralow amounts of DNA from long-term archived serum samples produce quality genotypes
Authors: Rounge Trine B. , Lauritzen Marianne , Erlandsen Sten Even , Langseth Hilde , Holmen Oddgeir Lingaas , Gislefoss Randi E. 
Published: November 2019

AbstractWhile genotyping studies are scavenging for suitable samples to analyze, large serum collections are currently left unused as they are assumed to provide insufficient amounts of DNA for array-based genotyping. Long-term stored serum is considered to be difficult to genotype since preanalytical treatments and storage effects on DNA yields are not well understood. Successful genotyping of such samples has the potential to activate large biobanks for future genome-wide association studies (GWAS). We aimed to evaluate genotyping of ultralow amounts of DNA from samples stored up to 45 years in the Janus Serum Bank with two commercially available platforms. 64 samples, with various preanalytical treatments, were genotyped on the Axiom Array from Thermo Fisher Scientific and a subset of 24 samples with slightly higher yield were genotyped on the HumanCoreExome array from Illumina. Our results showed that about 80% of the serum samples produced call rates with the Axiom arrays that would be satisfactory in GWAS. The mean DNA yield was 5.8 ng as measured with PicoGreen, 3–6% of recommended yield. The failed samples had on average lower input amounts of DNA. All serum samples genotyped on the HumanCoreExome with a standard and FFPE protocol produced GWAS satisfactory call rates, with mean 97.57% and 98.35% call rates, respectively. The mean yield was 10.65 ng, 6% of the recommendations. Successful array-based genotyping of ultralow DNA yields from serum samples stored up to 45 years is possible. These results demonstrate the potential to activate large serum biobank collections for future studies.
Economic value of protected areas via visitor mental health
Authors: Buckley Ralf , Brough Paula , Hague Leah , Chauvenet Alienor , Fleming Chris , Roche Elisha , Sofija Ernesta , Harris Neil 
Published: November 2019

AbstractWe evaluate methods to calculate the economic value of protected areas derived from the improved mental health of visitors. A conservative global estimate using quality-adjusted life years, a standard measure in health economics, is US$6 trillion p.a. This is an order of magnitude greater than the global value of protected area tourism, and two to three orders greater than global aggregate protected area management agency budgets. Future research should: refine this estimate using more precise methods; consider interactions between health and conservation policies and budgets at national scales; and examine links between personalities and protected area experiences at individual scale.
Quantum oscillations of robust topological surface states up to 50 K in thick bulk-insulating topological insulator
Authors: Zhao Weiyao , Chen Lei , Yue Zengji , Li Zhi , Cortie David , Fuhrer Michael , Wang Xiaolin 
Published: November 2019

AbstractAs personal electronic devices increasingly rely on cloud computing for energy-intensive calculations, the power consumption associated with the information revolution is rapidly becoming an important environmental issue. Several approaches have been proposed to construct electronic devices with low-energy consumption. Among these, the low-dissipation surface states of topological insulators (TIs) are widely employed. To develop TI-based devices, a key factor is the maximum temperature at which the Dirac surface states dominate the transport behavior. Here, we employ Shubnikov-de Haas oscillations (SdH) as a means to study the surface state survival temperature in a high-quality vanadium doped Bi1.08Sn0.02Sb0.9Te2S single crystal system. The temperature and angle dependence of the SdH show that: (1) crystals with different vanadium (V) doping levels are insulating in the 3–300 K region; (2) the SdH oscillations show two-dimensional behavior, indicating that the oscillations arise from the pure surface states; and (3) at 50 K, the V0.04 single crystals (Vx:Bi1.08-xSn0.02Sb0.9Te2S, where x = 0.04) still show clear sign of SdH oscillations, which demonstrate that the surface dominant transport behavior can survive above 50 K. The robust surface states in our V doped single crystal systems provide an ideal platform to study the Dirac fermions and their interaction with other materials above 50 K.
Enhanced biodegradation of n-hexane by Pseudomonas sp. strain NEE2
Authors: He Shanying , Ni Yaoqi , Lu Li , Chai Qiwei , Liu Haiyang , Yang Chunping 
Published: November 2019

AbstractPseudomonas sp. strain NEE2 isolated from oil-polluted soils could biodegrade n-hexane effectively. In this study, the secretory product of n-hexane biodegradation by NEE2 was extracted, characterized, and investigated on the secretory product’s enhanced effect on n-hexane removal. The effects of various biodegradation conditions on n-hexane removal by NEE2, including nitrogen source, pH value, and temperature were also investigated. Results showed that the secretory product lowered surface tension of water from 72 to 40 mN/m, with a critical micelle concentration of 340 mg/L, demonstrating that there existed biosurfactants in the secretory product. The secretory product at 50 mg/L enhanced n-hexane removal by 144.4% within 48 h than the control group. The optimum conditions for n-hexane removal by NEE2 were at temperature of 25–30 °C, pH value of 7–8, and (NH4)2SO4 as nitrogen source. Besides n-hexane, NEE2 could also utilize a variety of carbon sources. These results proved that NEE2 can consume hydrophobic volatile organic compounds (VOCs) to produce biosurfactants which can further enhance hydrophobic VOCs degradation.
SeeTB: A novel alternative to sputum smear microscopy to diagnose tuberculosis in high burden countries
Authors: Pandey Vikas , Singh Pooja , Singh Saumya , Arora Naresh , Quadir Neha , Singh Saurabh , Das Ayan , Dudeja Mridu , Kapur Prem , Ehtesham Nasreen Zafar , Elangovan Ravikrishnan , Hasnain Seyed E. 
Published: November 2019

AbstractMicroscopy-based tuberculosis (TB) diagnosis i.e. Ziehl-Neelsen screening still remains the primary diagnostic method in resource poor and high TB burden countries, however this method has poor sensitivity (~60%). Bringing three million TB patients who are left undiagnosed under the treatment has been a major focus as part of END-TB strategy across the world. We have developed a portable set-up called ‘SeeTB’ that converts a bright-field microscope into fluorescence microscope (FM) with minimal interventions. SeeTB, a total internal reflection-based fluorescence excitation system allows visualization of auramine-O stained bacilli efficiently with high signal-to-noise ratio. Along with the device, we have developed a sputum-processing reagent called ‘CLR’ that homogenizes and digests the viscous polymer matrix of sputum. We have compared the performance of SeeTB system in 237 clinical sputum samples along with FM, GeneXpert and liquid culture. In comparison with culture as gold standard, FM has sensitivity of 63.77% and SeeTB has improved sensitivity to 76.06%. In comparison with GeneXpert, FM has sensitivity of 73.91% while SeeTB has improved sensitivity to 85.51%. However, there is no significant change in the specificity between FM and SeeTB system. In short, SeeTB system offers the most realistic option for improved TB case identification in resource-limited settings.