bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteogenomic analysis of Inhibitor of Differentiation 4 (ID4) in basal-like breast cancer
Authors: Baker Laura A. , Holliday Holly , Roden Daniel , Krisp Christoph , Wu Sunny Z. , Junankar Simon , Serandour Aurelien A. , Mohammed Hisham , Nair Radhika , Sankaranarayanan Geetha , Law Andrew M. K. , McFarland Andrea , Simpson Peter T. , Lakhani Sunil , Dodson Eoin , Selinger Christina , Anderson Lyndal , Samimi Goli , Hacker Neville F. , Lim Elgene , Ormandy Christopher J. , Naylor Matthew J. , Simpson Kaylene , Nikolic Iva , O’Toole Sandra , Kaplan Warren , Cowley Mark J. , Carroll Jason S. , Molloy Mark , Swarbrick Alexander 
Published: June 2020

Abstract
Background
Basal-like breast cancer (BLBC) is a poorly characterised, heterogeneous disease. Patients are diagnosed with aggressive, high-grade tumours and often relapse with chemotherapy resistance. Detailed understanding of the molecular underpinnings of this disease is essential to the development of personalised therapeutic strategies. Inhibitor of differentiation 4 (ID4) is a helix-loop-helix transcriptional regulator required for mammary gland development. ID4 is overexpressed in a subset of BLBC patients, associating with a stem-like poor prognosis phenotype, and is necessary for the growth of cell line models of BLBC through unknown mechanisms.

Methods
Here, we have defined unique molecular insights into the function of ID4 in BLBC and the related disease high-grade serous ovarian cancer (HGSOC), by combining RIME proteomic analysis, ChIP-seq mapping of genomic binding sites and RNA-seq.

Results
These studies reveal novel interactions with DNA damage response proteins, in particular, mediator of DNA damage checkpoint protein 1 (MDC1). Through MDC1, ID4 interacts with other DNA repair proteins (γH2AX and BRCA1) at fragile chromatin sites. ID4 does not affect transcription at these sites, instead binding to chromatin following DNA damage. Analysis of clinical samples demonstrates that ID4 is amplified and overexpressed at a higher frequency in BRCA1-mutant BLBC compared with sporadic BLBC, providing genetic evidence for an interaction between ID4 and DNA damage repair deficiency.

Conclusions
These data link the interactions of ID4 with MDC1 to DNA damage repair in the aetiology of BLBC and HGSOC.
Tick-borne encephalitis virus (TBEV) prevalence in field-collected ticks (Ixodes ricinus) and phylogenetic, structural and virulence analysis in a TBE high-risk endemic area in southwestern Germany
Authors: Ott Daniela , Ulrich Kristina , Ginsbach Philip , Öhme Rainer , Bock-Hensley Oswinde , Falk Ulrich , Teinert Martina , Lenhard Thorsten 
Published: June 2020

Abstract
Background
Tick-borne encephalitis (TBE) is the most common viral CNS infection with incidences much higher than all other virus infections together in many risk areas of central and eastern Europe. The Odenwald Hill region (OWH) in southwestern Germany is classified as a TBE risk region and frequent case numbers but also more severe infections have been reported within the past decade. The objective of the present study was to survey the prevalence of tick-borne encephalitis virus (TBEV) in Ixodes ricinus and to associate TBEV genetic findings with TBE infections in the OWH.

Methods
Ticks were collected by the flagging methods supported by a crowdsourcing project implementing the interested public as collectors to cover completely and collect randomly a 3532 km2 area of the OWH TBE risk region. Prevalence of TBEV in I. ricinus was analysed by reversed transcription quantitative real-time PCR. Phylogeographic analysis was performed to classify OWH TBEV isolates within a European network of known TBEV strains. Mutational sequence analysis including 3D modelling of envelope protein pE was performed and based on a clinical database, a spatial association of TBE case frequency and severity was undertaken.

Results
Using the crowd sourcing approach we could analyse a total of 17,893 ticks. The prevalence of TBEV in I. ricinus in the OWH varied, depending on analysed districts from 0.12% to 0% (mean 0.04%). Calculated minimum infection rate (MIR) was one decimal power higher. All TBEV isolates belonged to the European subtype. Sequence analysis revealed a discontinuous segregation pattern of OWH isolates with two putative different lineages and a spatial association of two isolates with increased TBE case numbers as well as exceptional severe to fatal infection courses.

Conclusions
TBEV prevalence within the OWH risk regions is comparatively low which is probably due to our methodological approach and may more likely reflect prevalence of natural TBEV foci. As for other European regions, TBEV genetics show a discontinuous phylogeny indicating among others an association with bird migration. Mutations within the pE gene are associated with more frequent, severe and fatal TBE infections in the OWH risk region.
Flight of the COVID-19 patient: experience with a Wuhan evacuee: a case report
Authors: Segar Sandeep , Bouland Daniel , Torriani Francesca , Kwak Kevin , Asudani Deepak , Taplitz Randy , Gupta Vineet 
Published: June 2020

Abstract
Background
Coronavirus disease 2019, caused by severe acute respiratory syndrome coronavirus 2, was declared a global pandemic by the World Health Organization in March 2020.

Case presentation
We report a case of a 51-year-old Chinese woman who was evacuated from Wuhan, China and diagnosed with coronavirus disease 2019 infection at a Southern California quarantine facility. Her clinical course was notable for high fevers, night sweats, productive cough, transient leukopenia, lymphopenia, thrombocytopenia, and transaminitis. Evolving hypoxia and infiltrates on chest imaging warranted the trial of an investigational antiviral drug - remdesivir. Our patient recovered and was discharged after 2 weeks of hospitalization.

Conclusions
This case highlights our patient’s clinical course, including diagnostic work-up, medical management, and challenges in defining non-infectivity in a relatively unknown disease.
MiR-101-containing extracellular vesicles bind to BRD4 and enhance proliferation and migration of trophoblasts in preeclampsia
Authors: Cui Jinhui , Chen Xinjuan , Lin Shuo , Li Ling , Fan Jianhui , Hou Hongying , Li Ping 
Published: June 2020

Abstract
Background
Preeclampsia (PE) is a frequently occurring pregnancy disorder in the placenta, which results in various maternal and fetal complications. The current study aims to evaluate the role of extracellular vesicles (EVs)-encapsulated microRNA (miR)-101 in biological processes of trophoblasts in PE and its underlying mechanism.

Methods
Human umbilical cord mesenchymal stem cell (HUCMSC) and HUCMSC-derived EVs were isolated and cultured, after which EV characterization was carried out using PKH67 staining. In silico analyses were adopted to predict the downstream target genes of miR-101, and dual luciferase reporter gene assay was applied to validate the binding affinity. Furthermore, loss- and gain-of-function approaches were adopted to determine the role of miR-101 and bromodomain-containing protein 4 (BRD4) in trophoblast proliferation and invasion using EDU staining and transwell assay. In addition, a rat model of PE was established to verify the function of EV-encapsulated miR-101 in vivo.

Results
Placental tissues obtained from PE patients presented with downregulated miR-101 expression and upregulated BRD4 and CXCL11 expression. EV-encapsulated miR-101 from HUCMSCs could be delivered into the trophoblast HTR-8/SVneo cells, thus enhancing proliferation and migration of trophoblasts. Mechanically, miR-101 targeted and negatively regulated BRD4 expression. BRD4 knockdown promoted the proliferation and migration of trophoblasts by suppressing NF-κB/CXCL11 axis. EV-encapsulated miR-101 from HUCMSCs also reduced blood pressure and 24 h urine protein in vivo, thereby ameliorating PE.

Conclusion
In summary, EV-encapsulated miR-101 promoted proliferation and migration of placental trophoblasts through the inhibition of BRD4 expression via NF-κB/CXCL11 inactivation.
Leftover opioids following adult surgical procedures: a systematic review and meta-analysis
Authors: Schirle Lori , Stone Amanda L. , Morris Matthew C. , Osmundson Sarah S. , Walker Philip D. , Dietrich Mary S. , Bruehl Stephen 
Published: June 2020

AbstractBackgroundUS opioid prescribing and use escalated over the last two decades, with parallel increases in opioid misuse, opioid-related deaths, and concerns about diversion. Postoperatively prescribed opioids contribute to these problems. Policy makers have addressed this issue by limiting postoperative opioid prescribing. However, until recently, little data existed to guide prescribers on opioid needs postoperatively. This meta-analysis quantitatively integrated the growing literature regarding extent of opioids leftover after surgery and identified factors associated with leftover opioid proportions.MethodsWe conducted a meta-analysis of observational studies quantifying postoperative opioid consumption in North American adults, and evaluated effect size moderators using robust variance estimation meta-regression. Medline, EMBASE, Cumulative Index of Nursing and Allied Health Literature, and Cochrane Database of Systematic Reviews were searched for relevant articles published January 1, 2000 to November 10, 2018. The Methodological Index for Non-Randomized Studies (MINORS) tool assessed risk of study bias. The proportion effect size quantified the primary outcome: proportion of prescribed postoperative opioids leftover at the time of follow-up. Primary meta-regression analyses tested surgical type, amount of opioids prescribed, and study publication year as possible moderators. Secondary meta-regression models included surgical invasiveness, age, race, gender, postoperative day of data collection, and preoperative opioid use.ResultsWe screened 911 citations and included 44 studies (13,068 patients). The mean weighted effect size for proportion of postoperative opioid prescriptions leftover was 61% (95% CI, 56-67%). Meta-regression models revealed type of surgical procedure and level of invasiveness had a statistically significant effect on proportion of opioids leftover. Proportion of opioids leftover was greater for “other soft tissue” surgeries than abdominal/pelvic surgeries, but did not differ significantly between orthopedic and abdominal/pelvic surgeries. Minimally invasive compared to open surgeries resulted in a greater proportion of opioids leftover. Limitations include predominance of studies from academic settings, inconsistent reporting of confounders, and a possible publication bias toward studies reporting smaller leftover opioid proportions.Conclusions and implications of key findingsA significant proportion of opioids are leftover postoperatively. Surgery type and level of invasiveness affect postoperative opioid consumption. Integration of such factors into prescribing guidelines may help minimize opioid overprescribing while adequately meeting analgesic needs.
Contingency management for smoking cessation among individuals with type 2 diabetes: protocol for a multi-center randomized controlled feasibility trial
Authors: Martinez Sydney A. , Quaife Samantha L. , Hasan Afsheen , McMillan Kathryn A. , Beebe Laura A. , Muirhead Fiona 
Published: June 2020

Abstract
Background
Individuals with type 2 diabetes (T2D) who smoke are at increased risk for many types of cancers as well as an accelerated progression of microvascular and macrovascular complications. Smoking cessation is recommended as a standard treatment for T2D; however, individuals with T2D are faced with competing lifestyle changes. Glycemic and blood pressure control often take precedence over smoking cessation, and patients are often unmotivated to quit. Contingency management in combination with standard smoking cessation treatment has been demonstrated to improve cessation outcomes in various populations. The purpose of this randomized controlled feasibility trial is to explore the feasibility of contingency management and biochemical verification using a remote smartphone-based carbon monoxide monitor for smoking cessation among individuals with T2D.

Methods
A three-arm, randomized controlled feasibility trial will be conducted in two study sites that include the USA and UK. We will recruit 60 participants who will each receive usual care smoking cessation treatment (counseling and nicotine replacement therapy) and be randomized to a short term incentives (6 weeks), long term incentives (12 weeks), or no incentives (control) group. Participants will receive a smartphone and carbon monoxide monitor to complete daily remote assessments throughout the 12 weeks and will complete an exit interview at the end of the study. The primary outcomes for this feasibility study include completion of the protocol and proportion of daily assessments completed. Secondary outcomes include recruitment measures, acceptability, and smoking abstinence.

Discussion
We will explore the feasibility of recruiting smokers with T2D and their engagement in the program, particularly related to the use of the remote biochemical verification and smartphone application. In addition, we will evaluate the intervention content, study procedures, data collection methods, and follow-up and will qualitatively assess the participants’ acceptability of the program. The results of this study will inform the design of a larger trial to test the efficacy of the contingency management program for improving smoking cessation outcomes among individuals with T2D.

Trial registration
This randomized controlled feasibility trial has been registered at ClinicalTrials.gov with an ID NCT03527667 on May 4, 2018.
Anesthetic management of a patient with musculocontractural Ehlers-Danlos syndrome undergoing scoliosis surgery
Authors: Wakabayashi Ryo , Tanaka Satoshi , Tsuchiyama Keiko , Yamamoto Katsumi , Maruyama Yuki , Numata Kaori , Kawamata Mikito 
Published: June 2020

AbstractBackgroundMusculocontractural Ehlers-Danlos syndrome is a new and rare subtype of Ehlers-Danlos syndrome in which anesthetic considerations for airway and respiratory management, prevention of skin injuries and joint dislocations, and hemostatic management for severe perioperative bleeding are required.Case presentationA 19-year-old woman with musculocontractural Ehlers-Danlos syndrome was scheduled to undergo posterior spinal fusion from the 4th thoracic to the 4th lumbar vertebrae under general anesthesia. Her trachea was easily intubated with a videolaryngoscope despite a small mouth and micrognathia. Pressure-controlled ventilation with limited peak inspiratory pressure was performed for prevention of pneumothorax. Skin damage and joint luxation were prevented by using a low rebounding mattress, terpolymer-based barrier film, and careful patient positioning. Blood transfusion was effectively performed on the basis of point-of-care viscoelastic hemostatic assay monitoring. She had an uneventful postoperative course without any complications.ConclusionsWe safely managed a patient with musculocontractural Ehlers-Danlos syndrome undergoing scoliosis surgery.
Opioid-free anesthesia for a child with trisomy 13 with obstructive sleep apnea: a case report
Authors: Yamamoto Makiko , Miyazaki Izumi , Kishikawa Hiroaki , Sakamoto Atsuhiro 
Published: June 2020

AbstractBackgroundMost children with trisomy 13 display central apnea, and are prone to opioid-induced respiratory depression. We conducted opioid-free anesthesia for a patient with trisomy 13 and obstructive sleep apnea, and safely extubated the patient in the operating room.Case presentationA 27-month-old girl with trisomy 13 underwent tonsillectomy. Given her high sensitivity to opioids, general anesthesia was introduced and maintained only with 2–5% sevoflurane and 33% nitrous oxide in oxygen. We used acetaminophen for postoperative analgesia. The tracheal tube was removed under stable breathing pattern 10 min after the surgery in the operating room. Two years later, opioid-free anesthesia with 2–5% sevoflurane and 33% nitrous oxide in oxygen was again performed safely for tube insertion into both eardrums.ConclusionOpioid-free anesthesia with adequate non-narcotic analgesics is safe for children with trisomy 13 with multiple apnea-related comorbidities.
Emotional judgments of scenes are influenced by unintentional averaging
Authors: Alwis Yavin , Haberman Jason M. 
Published: June 2020

Abstract
Background
The visual system uses ensemble perception to summarize visual input across a variety of domains. This heuristic operates at multiple levels of vision, compressing information as basic as oriented lines or as complex as emotional faces. Given its pervasiveness, the ensemble unsurprisingly can influence how an individual item is perceived, and vice versa.

Methods
In the current experiments, we tested whether the perceived emotional valence of a single scene could be influenced by surrounding, simultaneously presented scenes. Observers first rated the emotional valence of a series of individual scenes. They then saw ensembles of the original images, presented in sets of four, and were cued to rate, for a second time, one of four.

Results
Results confirmed that the perceived emotional valence of the cued image was pulled toward the mean emotion of the surrounding ensemble on the majority of trials, even though the ensemble was task-irrelevant. Control experiments and analyses confirmed that the pull was driven by high-level, ensemble information.

Conclusion
We conclude that high-level ensemble information can influence how we perceive individual items in a crowd, even when working memory demands are low and the ensemble information is not directly task-relevant.
Effects of gonadotropin-releasing hormone agonist (GnRH-a) as luteal phase support in intracytoplasmic sperm injection (ICSI) cycles: a randomized controlled trial
Authors: Saharkhiz Nasrin , Salehpour Saghar , Hosseini Sedighe , Hosseinirad Hossein , Nazari Leila 
Published: June 2020

Abstract
Background
This paper describes a blind randomized controlled trial (RCT) designed to evaluate the effect of gonadotropin-releasing hormone agonist (GnRH-a) administration on outcomes of intracytoplasmic sperm injection (ICSI) in subjects stimulated with the gonadotropin-releasing hormone (GnRH) antagonist protocol. A total of 268 women who underwent ICSI cycles with GnRH antagonist ovarian stimulation protocol were included in the study. Patients were randomly assigned to the intervention (GnRH-a) and control groups. The intervention group received a single dose injection of triptorelin (0.1 mg) subcutaneously 6 days after oocyte retrieval while the control group received placebo. The rates of chemical and clinical pregnancy were defined as the primary outcome values.

Results
Two hundred forty participants accomplished the study, and their data were analyzed. No significant difference was detected between the chemical pregnancy rates of the intervention and control groups. However, the clinical pregnancy rate was significantly higher in the GnRH-a group than in the placebo group.

Conclusions
The findings of the present study suggest that the GnRH-a support in the luteal phase can result in a significant improvement of pregnancy rates in ICSI cycles following the ovarian stimulation with GnRH antagonist protocol.
Broadband finite-difference Q-compensated reverse time migration algorithm for tilted transverse isotropic media
Authors: Zhou Tong , Hu Wenyi , Ning Jieyuan 
Published: June 2020

[Formula: see text]-compensated reverse time migration (Q-RTM) is effective for improving the seismic imaging quality degraded by low-[Formula: see text] anomalies. However, it is difficult to apply existing pseudospectral-based Q-RTM methods to large-scale problems due to the obstacles to high-efficient parallelization posed by the global pseudospectral operators. On the other hand, finite-difference Q-RTM is intrinsically appropriate for domain decomposition and parallel computation, thus being suitable for industrial-sized problems but facing a twofold challenge: (1) to effectively compensate the phase in a broad-bandwidth sense during the wave back-propagation process and (2) to accurately handle the tilted transverse isotropic (TTI) medium with attenuation. We have developed a new framework of finite-difference Q-RTM algorithm by expanding the linear viscoacoustic constitutive relation to a series of integer-order differential terms and a unique integral term that can decouple the amplitude and the phase to allow accurate compensation in a broader frequency range. This framework has two typical implementations: (1) optimizing the frequency-dependent phase velocity (while fixing the negative constant [Formula: see text]) and (2) optimizing the [Formula: see text] value (while fixing the frequency-dependent phase velocity). We generalize this broadband finite-difference Q-RTM algorithm to TTI media, where an artificial [Formula: see text] is applied to suppress the shear-wave artifacts induced by the acoustic TTI approximation. Numerical examples demonstrate that this Q-RTM method accurately compensates the phase and amplitude in a broad frequency range of 5–70 Hz and produces high-quality images. Due to the local nature of finite-difference operators, this algorithm is expected to outperform the existing pseudospectral-based Q-RTM methods in terms of computational efficiency and implementation convenience for real-world Q-RTM projects.
Metabolic Effects of Late Dinner in Healthy Volunteers—A Randomized Crossover Clinical Trial
Authors: Gu Chenjuan , Brereton Nga , Schweitzer Amy , Cotter Matthew , Duan Daisy , Børsheim Elisabet , Wolfe Robert R , Pham Luu V , Polotsky Vsevolod Y , Jun Jonathan C 
Published: June 2020

AbstractContextConsuming calories later in the day is associated with obesity and metabolic syndrome. We hypothesized that eating a late dinner alters substrate metabolism during sleep in a manner that promotes obesity.ObjectiveThe objective of this work is to examine the impact of late dinner on nocturnal metabolism in healthy volunteers.Design and SettingThis is a randomized crossover trial of late dinner (LD, 22:00) vs routine dinner (RD, 18:00), with a fixed sleep period (23:00-07:00) in a laboratory setting.ParticipantsParticipants comprised 20 healthy volunteers (10 male, 10 female), age 26.0 ± 0.6 years, body mass index 23.2 ± 0.7 kg/m2, accustomed to a bedtime between 22:00 and 01:00.InterventionsAn isocaloric macronutrient diet was administered on both visits. Dinner (35% daily kcal, 50% carbohydrate, 35% fat) with an oral lipid tracer ([2H31] palmitate, 15 mg/kg) was given at 18:00 with RD and 22:00 with LD.Main Outcome MeasuresMeasurements included nocturnal and next-morning hourly plasma glucose, insulin, triglycerides, free fatty acids (FFAs), cortisol, dietary fatty acid oxidation, and overnight polysomnography.ResultsLD caused a 4-hour shift in the postprandial period, overlapping with the sleep phase. Independent of this shift, the postprandial period following LD was characterized by higher glucose, a triglyceride peak delay, and lower FFA and dietary fatty acid oxidation. LD did not affect sleep architecture, but increased plasma cortisol. These metabolic changes were most pronounced in habitual earlier sleepers determined by actigraphy monitoring.ConclusionLD induces nocturnal glucose intolerance, and reduces fatty acid oxidation and mobilization, particularly in earlier sleepers. These effects might promote obesity if they recur chronically.