bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detailed Dynamic Modelling of an Integrated Hydraulic Actuator
Authors: Wilson Dom , Georgilas Ioannis , Plummer Andrew , Iravani Pejman , Sangiah Dhinesh 
Published: October 2020

Abstract
Hydraulic servos are characterised by their high-performance nature but due to their size and weight are not suitable for robotics where new legged applications require high power density and excellent dynamic behaviour in a small size. As an answer to this need a new class of integrated smart actuators is being developed. These systems consist of a servo valve, hydraulic cylinder, sensors and a controller all in a single device. This paper outlines the detailed modelling of the smart actuator for use in simulation and control design. The result is a model consisting of the dynamics of the novel ultra-low leakage servovalve, the valve flow characteristics considering the properties of each spool land, the single-ended cylinder with friction and the pressure losses in the supply and return lines to the actuator. The models are a combination of empirical and theoretical development, validated with experimental data. The smart actuator’s unique properties; compactness, weight and efficiency, combined with high-performance hydraulics make it well suited to mobile robot applications.
Sabkha dolomite as an archive for the magnesium isotope composition of seawater
Authors: Shalev Netta , Bontognali Tomaso R.R. , Vance Derek 
Published: October 2020

Abstract
Recent studies have uncovered the potential of Mg isotopes (δ26Mg) for studying past ocean chemistry, but records of such data are still scarce. Dolomite has been suggested as a promising archive for δ26Mg of seawater. However, its enigmatic formation mechanism and the difficulty in precipitating dolomite in the laboratory at surface temperatures decrease confidence in the interpretation of δ26Mg values from the rock record. To evaluate factors determining the δ26Mg of dolomite, we studied pore water and sediment from Dohat Faishakh Sabkha, Qatar—one of the rare environments where dolomite is currently forming. The δ26Mg values of the dolomite (−2.56‰ to −1.46‰) are lower than that of seawater (−0.83‰), whereas δ26Mg values of pore water (−0.71‰ to −0.14‰) are higher. The isotope fractionation accompanying dolomite formation is generally in accordance with an empirical fractionation from the literature, extrapolated to the sabkha’s temperature (−1.84‰ to −1.51‰). The results suggest that evaporated seawater is the sole source of Mg, and isotopically light dolomite is the major sink, so that the δ26Mg of the dolomite-forming pore water is equal to or greater than that of seawater. Thus, provided that the lowest δ26Mg value among several dolomite samples is used, and the formation temperature is known, similar sabkha-type dolomites can be utilized as an archive for δ26Mg values of ancient seawater.
Ice-rafted dropstones in “postglacial” Cryogenian cap carbonates
Authors: Le Heron D.P. , Busfield M.E. , Kettler C. 
Published: October 2020

AbstractDropstones of ice-rafted origin are typically cited as key cold-climate evidence in Cryogenian strata and, according to conventional wisdom, should not occur in postglacial, warm-water carbonates. In Namibia, the Chuos Formation (early Cryogenian) contains abundant dropstone-bearing intervals and striated clasts. It is capped by the Rasthof Formation, composed of laminites in its lower portion and microbial carbonates above. These laminites are locally found to contain pebble- and granule-sized lonestones in abundance. At the Omutirapo outcrop, meter-thick floatstone beds occur at the flanks of a Chuos paleovalley and are readily interpreted as mass-flow deposits. At Rasthof Farm, however, the clasts warp, deflect, and penetrate hundreds of carbonate laminations at both the outcrop and thin-section scale. We propose that these are dropstones, and we infer an ice-rafting mechanism. Evidence for vestigial glaciation concomitant with cap carbonate deposition thus merits a reappraisal of the depositional conditions of cap carbonates and their paleoclimatic significance.
Molecular Dialog between Root and Shoot via Regulatory Peptides and Its Role in Systemic Control of Plant Development
Authors: Lebedeva M. A. , Yashenkova Ya. S. , Dodueva I. E. , Lutova L. A. 
Published: October 2020

AbstractPlant developmental processes are very flexible and highly depend on environmental factors. This is largely due to the existence of regulatory mechanisms that systemically control development on the whole plant level. In plants, regulatory peptides produced in the roots have been identified that are activated in response to different factors influencing root system, such as variation in the level of macronutrients (first of all, nitrogen and phosphorus) in the soil, influence of symbiotic microorganisms (soil rhizobial bacteria and arbuscular mycorrhiza fungi), and water deficiency. Among the systemically acting peptides, the most thoroughly investigated are CLE (CLAVATA3/EMBRYO SURROUNDING REGION-related) and CEP (C-TERMINALLY ENCODED PEPTIDES) peptides that are capable of travelling through the xylem from the roots to the shoot and triggering responses via binding to specific receptors operating in the phloem of the leaf. This review focuses on the role of these two groups of peptides in molecular dialog between the root and shoot.
Orai-IGFBP3 signaling complex regulates high-glucose exposure-induced increased proliferation, permeability, and migration of human coronary artery endothelial cells
Authors: Bai Suwen , Wei Yuan , Hou Wenxuan , Yao YanHeng , Zhu Junwei , Hu Xianyu , Chen Wei , Du Yinan , He Wei , Shen Bing , Du Juan 
Published: October 2020

IntroductionDiabetes-associated endothelium dysfunction might be linked to disturbances in Ca2+homeostasis. Our main objective is to reveal the potential mechanisms by which high-glucose (HG) exposure promotes increased proliferation of human coronary artery endothelial cells (HCAECs) in culture, and that store-operated Ca2+entry (SOCE) and insulin-like growth factor binding protein 3 (IGFBP3) contribute to this proliferation.Research design and methodsWe detected the expression levels of Ca2+release-activated calcium channel proteins (Orais), IGFBP3 and proliferating cell nuclear antigen of HCAECs cultured in HG medium for 1, 3, 7, and 14 days and in streptozotocin-induced diabetic mouse coronary endothelial cells. Coimmunoprecipitation and immunofluorescence technologies were used to detect the interactions between Orais and IGFBP3 of HCAECs exposed to HG environment, and to detect IGFBP3 expression and proliferation after treatment of HCAECs cultured in HG medium with an agonist or inhibitor of SOCE. Similarly, after transfection of specific small interfering RNA to knock down IGFBP3 protein expression, SOCE activity and Orais expression were tested. Some processes related to endothelial dysfunction, such as migration, barrier function and adhesion marker expression, are also measured.ResultsHG exposure promoted increased proliferation of HCAECs in culture and that SOCE and IGFBP3 contributed to this proliferation. In addition, we also found that Orais and IGFBP3 were physically associated and regulated each other’s expression levels. Besides, their expression levels and interactions were enhanced in HCAECs after exposure to HG. HG exposure promotes cell migration, but reduces barrier function and adherens junction protein expression levels in HCAECs.ConclusionOrais and IGFBP3 formed a signaling complex that mediated HCAEC proliferation during HG exposure in culture. Meanwhile, we also found that SOCE stimulates proliferation of HCAECs by regulating IGFBP3, thereby promoting the occurrence and progression of coronary atherosclerosis in diabetes. It is worth noting that our findings may shed new light on the mechanisms of increased proliferation in HCAECs in diabetes and suggest the potential value of SOCE and IGFBP3 as therapeutic targets for coronary atherosclerosis in individuals with diabetes.
Retrospective chart review and survey to identify adverse safety events in the emergency medical services care of children with out-of-hospital cardiac arrest in the USA: a study protocol
Authors: Eriksson Carl , Schoonover Amanda , Harrod Tabria , Meckler Garth , Hansen Matt , Yanez David , Daya Mo , Jui Jonathan , Guise Jeanne-Marie 
Published: October 2020

IntroductionEfforts to improve the quality of emergency medical services (EMS) care for adults with out-of-hospital cardiac arrest (OHCA) have led to improved survival over time. Similar improvements have not been observed for children with OHCA, who may be at increased risk for preventable adverse safety events during prehospital care. The purpose of this study is to identify patient and organisational factors that are associated with adverse safety events during the EMS care of paediatric OHCA.Methods and analysisThis is a large multisite EMS study in the USA consisting of chart reviews and agency surveys to measure, characterise and evaluate predictors of our primary outcome severe adverse safety events in paediatric OHCA. Using the previously validated Paediatric prehospital adverse Event Detection System tool, we will review EMS charts for 1500 children with OHCA from 2013 to 2019 to collect details of each case and identify severe adverse safety events (ASEs). Cases will be drawn from over 40 EMS agencies in at least five states in geographically diverse areas of the USA. EMS agencies providing charts will also be invited to complete an agency survey to capture organisational characteristics. We will describe the frequency and proportion of severe ASEs in paediatric OHCA across geographic regions and clinical domains, and identify patient and EMS organisational characteristics associated with severe ASEs using logistic regression.Ethics and disseminationThis study has been approved by the Oregon Health & Science University Institutional Review Board (IRB Approval# 00018748). Study results will be disseminated through scientific publications and presentations, and to EMS leaders and staff through local EMS medical directors, quality and training officers and community engagement activities.
Brief compression-only cardiopulmonary resuscitation and automated external defibrillator course for secondary school students: a multischool feasibility study
Authors: So Kit Ying , Ko Hiu Fai , Tsui Cindy Sin Yui , Yeung Chi Yeung , Chu Yee Ching , Lai Veronica Ka Wai , Lee Anna 
Published: October 2020

ObjectivesThis study assessed the feasibility and preliminary efficacy of a 2-hour compression-only cardiopulmonary resuscitation and automated external defibrillator (CO-CPRAED) course in secondary school students.DesignProspective pre-post feasibility study.Setting and participants128 students (12–15 years old) without prior basic life support (BLS) training at four secondary schools in Hong Kong. All students were followed up at 3 months after training.InterventionsEmergency medicine-trained nurse and physicians taught the 2-hour CO-CPRAED course using the American Heart Association ‘CPR in School Training Kit’ programme. Students were trained in groups up to 40 students/session, with an instructor to student ratio not exceeding 1:10. To practise hands-on compressions, the manikin to student ratio was 1:1. For a simulated cardiac arrest, the manikin and AED to student ratio was 1:10.Primary and secondary outcomesCPR and AED knowledge, attitude statements towards bystander CPR and AED, quality of BLS performance skills during training and at 3 months.ResultsSome students (46%) knew how deep to push on an adult chest when doing CO-CPR before training. The course was associated with an increase in knowledge score (pretraining 55%, post-training 93%; adjusted mean difference (MD) 38%, 95% CI 33% to 43%; p<0.001). Most students (68%) thought that CPR education in senior secondary school was essential before training. The students had a very positive attitude towards CPR; no change in the mean (SD) attitude score out of 30 over time (pretraining 27.2 (2.5), post-training 27.6 (2.7); adjusted MD 0.5, 95% CI −0.1 to 1.0; p=0.132). Most students were competent in performing BLS immediately after training (77%) and at 3 months (83%) (adjusted MD 6%, 95% CI −4% to 15%; p=0.268).ConclusionsThe results demonstrate the feasibility of scaling up the number of secondary schools trained in a brief CO-CPRAED course within the local school curriculum.
Protecting the front line: a cross-sectional survey analysis of the occupational factors contributing to healthcare workers’ infection and psychological distress during the COVID-19 pandemic in the USA
Authors: Firew Tsion , Sano Ellen D , Lee Jonathan W , Flores Stefan , Lang Kendrick , Salman Kiran , Greene M Claire , Chang Bernard P 
Published: October 2020

ObjectiveThe COVID-19 pandemic has been associated with significant occupational stressors and challenges for front-line healthcare workers (HCWs), including COVID-19 exposure risk. Our study sought to assess factors contributing to HCW infection and psychological distress during the COVID-19 pandemic in the USA.DesignWe conducted a cross sectional survey of HCWs (physicians, nurses, emergency medical technicians (EMTs), non-clinical staff) during May 2020. Participants completed a 42-item survey assessing disease transmission risk (clinical role, work environment, availability of personal protective equipment) and mental health (anxiety, depression and burn-out).SettingThe questionnaire was disseminated over various social media platforms. 3083 respondents from 48 states, the District of Columbia and US territories accessed the survey.ParticipantsUsing a convenience sample of HCWs who worked during the pandemic, 3083 respondents accessed the survey and 2040 participants completed at least 80% of the survey.Primary outcomePrevalence of self-reported COVID-19 infection, in addition to burn-out, depression and anxiety symptoms.ResultsParticipants were largely from the Northeast and Southern USA, with attending physicians (31.12%), nurses (26.80%), EMTs (13.04%) with emergency medicine department (38.30%) being the most common department and specialty represented. Twenty-nine per cent of respondents met the criteria for being a probable case due to reported COVID-19 symptoms or a positive test. HCWs in the emergency department (31.64%) were more likely to contract COVID-19 compared with HCWs in the ICU (23.17%) and inpatient settings (25.53%). HCWs that contracted COVID-19 also reported higher levels of depressive symptoms (mean diff.=0.31; 95% CI 0.16 to 0.47), anxiety symptoms (mean diff.=0.34; 95% CI 0.17 to 0.52) and burn-out (mean diff.=0.54; 95% CI 0.36 to 0.71).ConclusionHCWs have experienced significant physical and psychological risk while working during the COVID-19 pandemic. These findings highlight the urgent need for increased support for provider physical and mental health well-being.
Absence of pontine perforators in vertebrobasilar dolichoectasia on ultra-high resolution cone-beam computed tomography
Authors: Dobrocky Tomas , Piechowiak Eike I , Goldberg Johannes , Barvulsky Aleman Enrique , Nicholson Patrick , Lynch Jeremy , Bervini David , Kaesmacher Johannes , Agid Ronit , Krings Timo , Raabe Andreas , Gralla Jan , Pereira Vitor M , Mordasini Pasquale 
Published: October 2020

Background
Vertebrobasilar dolichoectasia (VBDE) is a rare type of non-saccular intracranial aneurysm, with poor natural history and limited effective treatment options. Visualizing neurovascular microanatomy in patients with VBDE has not been previously reported, but may yield insight into the pathology, and provide important information for treatment planning.

Objective
To carry out a retrospective analysis of ultra-high resolution cone-beam computed tomography (UHR-CBCT) in patients with fusiform basilar aneurysms, visualizing neurovascular microanatomy of the posterior circulation with a special focus on the pontine perforators.

Methods
UHR-CBCT was performed in seven patients (mean age 59 years; two female) with a VBDE, and in 14 control patients with unrelated conditions.

Results
The mean maximum diameter of the fusiform vessel segment was 28 mm (range 19–36 mm), and the mean length of the segment was 39 mm (range 15–50 mm). In all patients with VBDE, UHR-CBCT demonstrated an absence of perforating arteries in the fusiform arterial segment and a mean of 3.7 perforators arising from the unaffected vessel segment. The network of interconnected superficial circumferential pontine arteries (brainstem vasocorona) were draping around the aneurysm sac. In controls, a mean of 3.6, 2.5, and 1.2 perforators were demonstrated arising from the distal, mid-, and proximal basilar artery, respectively.

Conclusions
The absence of pontine perforators in the fusiform vessel segment of VBDE is counterbalanced by recruitment of collateral flow from pontine perforators arising from the unaffected segment of the basilar artery, as well as collaterals arising from the anterior inferior cerebellar artery/posterior inferior cerebellar artery and superior cerebellar artery. These alternative routes supply the superficial brainstem arteries (brainstem vasocorona) and sustain brainstem viability. Our findings might have implications for further treatment planning.
Beta blockers versus calcium channel blockers for provocation of vasospastic angina after drug-eluting stent implantation: a multicentre prospective randomised trial
Authors: Sawano Mitsuaki , Katsuki Toshiomi , Kitai Takeshi , Tamita Koichi , Obunai Kotaro , Ikegami Yukinori , Yamane Takafumi , Ueda Ikuko , Endo Ayaka , Maekawa Yuichiro , Kawamura Akio , Fukuda Keiichi , Kohsaka Shun 
Published: October 2020

BackgroundDrug-eluting stent-induced vasospastic angina (DES-VSA) has emerged as a novel complication in the modern era of percutaneous coronary intervention (PCI). Although beta blockers (BBs) are generally recommended for coronary heart disease, they may promote incidence of DES-VSA. This study aimed to compare the effects of calcium channel blockers (CCBs) perceived to be protective against DES-VSA and BBs on subsequent coronary events after second-generation drug-eluting stent implantation.MethodsIn this multicentre prospective, randomised study, 52 patients with coronary artery disease who underwent PCI for a single-vessel lesion with everolimus-eluting stent placement were randomised into post-stenting BB (N=26) and CCB (N=26) groups and followed for 24 months to detect any major cardiovascular events (MACE). A positive result on acetylcholine provocation testing during diagnostic coronary angiography (CAG) at 9 months was the primary endpoint for equivalence. MACE included all-cause death, non-fatal myocardial infarction, unstable angina, cerebrovascular disease or coronary revascularisation for stable coronary artery disease after index PCI.ResultsAt 9 months, 42 patients (80.8%) underwent diagnostic coronary angiography and acetylcholine provocation testing. Among them, seven patients in each group were diagnosed with definite vasospasm (intention-to-treat analysis 26.9% vs 26.9%, risk difference 0 (−0.241, 0.241)). Meanwhile, the secondary endpoint, 24-month MACE, was higher in the CCB group (19.2%) than in the BB group (3.8%) (p=0.01). In detail, coronary revascularisation for stable coronary artery disease was the predominant endpoint that contributed to the greater proportion of MACE in the CCB group (CCB (19.2%) vs BB (3.8%), p=0.03).ConclusionsThe incidence of acetylcholine-induced coronary artery spasms did not differ between patients receiving BBs or CCBs at 9 months after PCI. However, a higher incidence of 2-year MACE was observed in the CCB group, suggesting the importance of BB administration.Trial registration numberThis study was registered at the Japanese University Hospital Medical Information Network (UMIN) Clinical Trial Registry (The Prospective Randomized Trial for Optimizing Medical Therapy After Stenting: Calcium-Beta Trial; UMIN000008321,https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000009536).
New COL4A5 mutation in IgA nephropathy
Authors: Xu Zhenjian , Chen Junzhe , Yu Wenjuan , Li Xiaomei , Lin Baojuan , Lai Deyuan , Xu Anping , Tang Ying 
Published: October 2020

AbstractPurposeIgA nephropathy (IgAN) is the most common type of primary glomerulonephritis and a leading cause of chronic kidney disease (CKD) and end-stage kidney disease (ESKD). Recently, some case reports have shown that COL4A5 mutation is associated with IgAN. Here, we identified a new COL4A5 gene mutation in IgAN in a Chinese family.Materials and MethodsIn the present study, the proband and his 23-year-old younger brother were both diagnosed with IgAN, manifested as haematuria, proteinuria and chronic kidney injury without hearing loss or ocular symptoms. Additionally, the proband's 30-year-old younger brother, also diagnosed with ESKD, had been undergoing dialysis for 2 years with normal hearing and eyesight. To exclude genetic disease, we conducted whole-exome sequencing and Sanger sequencing assays.ResultsWe found a new mutation in the COL4A5 gene (chrX:107 814 698, c.438+2-&gt;AAACCAATTATA-), a novel insertion mutation. Using vector transcription and Minigene transcriptional analyses, we verified, for the first time, the novel mutation pathogenicity of the COL4A5 gene.ConclusionTogether with other published data, we suggest that genetic screening should be performed in IgAN, particularly for patients with a familial history. The effects of different mutated splice sites of the COL4A5 gene, as well as the tissue specificity of the splicing machinery contributing to the pathogenesis and prognosis of IgAN, remains unclear and warrants further exploration in the future.
Peritoneal Spread of Ovarian Cancer Harbors Therapeutic Vulnerabilities Regulated by FOXM1 and EGFR/ERBB2 Signaling
Authors: Parashar Deepak , Nair Bindu , Geethadevi Anjali , George Jasmine , Nair Ajay , Tsaih Shirng-Wern , Kadamberi Ishaque P. , Gopinadhan Nair Gopa Kumar , Lu Yiling , Ramchandran Ramani , Uyar Denise S. , Rader Janet S. , Ram Prahlad T. , Mills Gordon B. , Pradeep Sunila , Chaluvally-Raghavan Pradeep 
Published: October 2020

Abstract

Peritoneal spread is the primary mechanism of metastasis of ovarian cancer, and survival of ovarian cancer cells in the peritoneal cavity as nonadherent spheroids and their adherence to the mesothelium of distant organs lead to cancer progression, metastasis, and mortality. However, the mechanisms that govern this metastatic process in ovarian cancer cells remain poorly understood. In this study, we cultured ovarian cancer cell lines in adherent and nonadherent conditions in vitro and analyzed changes in mRNA and protein levels to identify mechanisms of tumor cell survival and proliferation in adherent and nonadherent cells. EGFR or ERBB2 upregulated ZEB1 in nonadherent cells, which caused resistance to cell death and increased tumor-initiating capacity. Conversely, Forkhead box M1 (FOXM1) was required for the induction of integrin β1, integrin-α V, and integrin-α 5 for adhesion of cancer cells. FOXM1 also upregulated ZEB1, which could act as a feedback inhibitor of FOXM1, and caused the transition of adherent cells to nonadherent cells. Strikingly, the combinatorial treatment with lapatinib [dual kinase inhibitor of EGFR (ERBB1) and ERBB2] and thiostrepton (FOXM1 inhibitor) reduced growth and peritoneal spread of ovarian cancer cells more effectively than either single-agent treatment in vivo. In conclusion, these results demonstrate that FOXM1 and EGFR/ERBB2 pathways are key points of vulnerability for therapy to disrupt peritoneal spread and adhesion of ovarian cancer cells.

Significance:
This study describes the mechanism exhibited by ovarian cancer cells required for adherent cell transition to nonadherent form during peritoneal spread and metastasis.