bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospective directions of treatment of metabolic dysfunction-associated steatotic liver disease. Focus on intestinal microbiota. Review
Authors: Fadieienko G. D. , Gridnyev O. Y. , Gridnyeva S. V. 
Published: December 2024

Taking into account the close connection of intestinal microbiota disorders with the pathogenetic links of metabolic dysfunction‑associated steatotic liver disease(MASLD), the application of therapeutic methods aimed at the modulation of microbiome becomes relevant. The most powerful factors affecting the intestinal microbiota are the nature of nutrition and the level of physical activity; therefore, dietary interventions and recommendations for physical activity, which are the main part of MASLD therapy, should be considered. In this aspect, interventions targeting the gut‑liver axis and the gut microbiome offer promising opportunities for the development of personalized and targeted therapies for MASLD. To date, many molecules have been developed that have a modulating effect on the intestinal microbiota, most of which are prebiotics, probiotics, and synbiotics. Various studies have shown that the use of probiotics is quite effective in the treatment of NAFLD by reducing endotoxinemia, the level of liver aminotransferases, the degree of liver steatosis, improving insulin sensitivity, normalizing lipid metabolism, and having a positive effect on hepatocyte mitochondria. Synbiotics, prebiotics, and phytochemicals with prebiotic activity have effects similar to probiotics in patients with MASLD — the growth of beneficial bacteria in the intestines, reduction of metabolic endotoxemia and systemic inflammation, relief of glucose and lipid metabolism disorders, liver steatosis. More and more clinical data indicate the positive effects of the use of fungal probiotics and antifungal agents in MASLD: reduction of fat mass and hyperinsulinemia, reduction of inflammation and liver fibrosis. Topical antibiotics also show interesting results due to their effect on the integrity of the intestinal barrier and their eubiotic properties. Transplantation of faecal microbiota is also considered as a potential therapeutic method for intestinal rabies in MASLD. The available clinical data of its use are very encouraging: reduction of glucose, insulin and glycated hemoglobin levels (albeit in the short term), reduction of increased permeability of the intestinal barrier and the degree of liver steatosis. However, there is still an urgent need in larger‑scale randomized studies to verify the results of the use of pre‑ and probiotics, transplantation of faecal microbiota, and mycobiotics to determine the optimal regimen, duration, and specific strains of microorganisms that provide the most significant benefits. New approaches, using modified probiotic bacteria and bacteriophages, are promising, but their role in clinical practice remains to be determined.
Imagining Nature–Culture Hybridity
Authors: Greaves Sofia , Doezema Tess 
Published: December 2024

Abstract
This article takes a historical coproductionist approach to examine the pivotal claim of novelty on which circular economy (CE) discourse is centered. CE proponents argue that it represents a paradigm shift: concepts from nature—most notably the efficiency of biological cycles posited to be “without waste”—can be productively applied to the linear economy, resulting in a novel partnership that will correct human–nature relationality. We bring a historical perspective to critically appraise this claim, providing examples of how concepts of circularity have been transmitted between medical, economic, and engineering texts across the history of modern thought. We show how these ideas have been coproduced with political agendas, acting as legitimizing forces for powerful normative claims while shaping collective imaginations of right moral order in nature and culture.
MATERIALIAÇÃO DA PROPOSTA DE AVALIAÇÃO NA FORMAÇÃO DE PROFESSORES DE QUÍMICA DA REDE FEDERAL
Authors: Oliveira Sabrina Kelly Andrade de , Vieira Manuelle Patrícia Ramos 
Published: December 2024

A presente pesquisa tem por objetivo compreender a materialização da avaliação na formação de professores de Química no tocante as prÁticas avaliativas do Projeto Pedagógico do Curso (PPC) e as Concepções de Avaliação, abordadas por Guba e Lincoln, com foco nas Gerações da Avaliação. Para a obtenção de dados foi feita uma exígua amostragem com a aplicação de um formulário contendo quatro questonionamentos sobre a avaliação em sua sala de aula para professores da Rede Federal de Ensino. Os resultados obtidos foram tabulados e analisados, permitindo a observação da conformidade, entre as palavras dos professores e o Projeto Pedagógico do Curso, relacionado a materialização deste e as múltiplas dimenssões da avaliação, compreendidas nas perspectivas formativa, diagnóstica e somativa. Após a análise percebeu-se uma inclinação dos respondentes a uma avaliação de segunda e terceira geração.
Comparative proteomic analysis of patients with acute myeloid leukemia before and after induction therapy
Authors: Restrepo-Rodríguez Luisa , Prada-Arismendy Jeanette , Castillo Erwing , Rothlisberger Sarah 
Published: December 2024

BACKGROUND: Acute myeloid leukemia (AML) is a malignant disorder of hematopoietic stem and progenitor cells, characterized by accumulation of immature blasts in the bone marrow and peripheral blood of affected patients. Response to chemotherapy treatment in patients with AML is wide-ranging, and to date there are no adequate molecular biomarkers used to predict clinical outcome. OBJECTIVE: The aim of this study was to identify potential protein biomarkers which could help predict response to induction treatment in AML patients. METHODS: Peripheral blood samples were obtained from 15 AML patients both before and after treatment. A comparative proteomic analysis was performed using 2D gel electrophoresis followed by Mass Spectrometry. RESULTS: This comparative proteomic study, combined with a protein network analysis, revealed several proteins that could be considered potential biomarkers of poor prognosis in AML: GAPDH which favors increased glucose metabolism; eEF1A1 and Annexin A1 that promote proliferation and migration, cofilin 1 which plays a role in the activation of apoptosis; and GSTP1 which is involved in the processes of detoxification and chemoresistance. CONCLUSIONS: This study gives an insight into a panel of protein biomarkers with prognostic potential that should be further investigated.
The effect of sodium fluorescein angiography on erythrocyte properties
Authors: Sargento L. , Zabala L. , Saldanha C. , Souza‐Ramalho P. , Martins e Silva J. 
Published: December 2024

Sodium fluorescein angiography is a widely used routine ophthalmological diagnostic procedure which enables the study of chorioretinal microcirculation and consists of the injection of sodium fluorescein into the systemic bloodstream. The aim of the present study was to evaluate whether or not fluorescein interferes with erythrocyte properties during the angiographic procedure. In a group of 37 patients, 26 with non‐insulin‐dependent diabetes mellitus (DM) with and without retinopathy, and 11 without diabetes mellitus (non‐DM) although affected by other ophthalmological diseases, all undergoing routine angiography, blood samples were drawn before (T $_0$ ) and 30 min (T $_{30}$ ) after fluorescein injection. The erythrocyte aggregation index (EAI), membrane lipid fluidity and erythrocyte acetylcholinesterase activity were determined in both groups. After fluorescein injection there was no statistical change in EAI and erythrocyte membrane fluidity in either group. Erythrocyte acetylcholinesterase activity, a marker of membrane protein integrity, decreased significantly ( $p<0.01$ ) in the DM group. Membrane lipid fluidity did not change with fluorescein injection, however, (i) in the DM group erythrocyte membranes became more rigid than in the non‐DM (DPH: $p<0.01$ ); (ii) EAI and membrane lipid fluidity became significantly correlated ( $r= 0.6263$ , $p<0.05$ ) in non‐DM patients at T $_{30}$ . In conclusion, fluorescein administration for angiographic procedures seems to interact with erythrocyte membrane, namely, in diabetic patients, which may interfere with the blood flow in the microcirculation.
Assessment of auditory temporal-order thresholds – A	comparison of different measurement procedures and the influences of age and	gender
Authors: Fink Martina , Churan Jan , Wittmann Marc 
Published: December 2024

Purpose: The relationship between auditory temporal-order perception and phoneme discrimination has been discussed for several years, based on findings, showing that patients with cerebral damage in the left hemisphere and aphasia, as well as children with specific language impairments, show deficits in temporal-processing and phoneme discrimination. Over the last years several temporal-order measurement procedures and training batteries have been developed. However, there exists no standard diagnostic tool for adults that could be applied to patients with aphasia. Therefore, our study aimed at identifying a feasible, reliable and efficient measurement procedure to test for auditory-temporal processing in healthy young and elderly adults, which in a further step can be applied to patients with aphasia. Methods: The tasks varied according to adaptive procedures (staircase vs. maximum-likelihood), stimuli (tones vs. clicks) and stimulation modes (binaural- vs. alternating monaural) respectively. A phoneme-discrimination task was also employed to assess the relationship between temporal and language processing. Results: The results show that auditory temporal-order thresholds are stimulus dependent, age related, and influenced by gender. Furthermore, the cited relationship between temporal-order threshold and phoneme discrimination can only be confirmed for measurements with pairs of tones. Conclusion: Our results indicate, that different norms have to be established for different gender and age groups. Furthermore, temporal-order measurements with tones seem to be more suitable for clinical intervention studies than measurements with clicks, as they show higher re-test reliabilities, and only for measurements with tones an association with phoneme-discrimination abilities was found.
Recovery of visual evoked potentials after regeneration of cut	retinal ganglion cell axons within the ascending visual pathway in adult	rats
Authors: Heiduschka Peter , Fischer Dietmar , Thanos Solon 
Published: December 2024

Purpose: Following optic nerve damage, retinal ganglion cells (RGCs) fail to regenerate their axons and soon undergo apoptosis. However, many RGCs survive axotomy and regenerate lengthy axons after a lens injury (LI). If the cut optic nerve is re-sutured, RGC axons grow into the distal part of the optic nerve and reach their natural targets within the thalamus and midbrain. In this study, we check time-dependence and extent of restoration of flash visual evoked potentials (FVEPs) to examine the functional relevance of the regenerated retinogeniculate pathway. Methods: The optic nerve in adult rats was cut and re-sutured. The lens was injured transsclerally using a pointed glass capillary. FVEPs were measured starting at the time point of surgery, and then repeatedly up to an age of several months. Results: Detectable FVEPs appeared approximately ten weeks after the surgery, and their amplitudes increased during the next months to reach eventually 15–40% of their values before surgery. Conclusions: Partial restoration of FVEPs indicates that some regenerating RGC axons have "bridged" the distance between the eye and the central targets forming a functional re-connection of the corresponding RGC with thalamic target neurones to elicit recordable activation of the visual cortex
The ability of physiological stimuli to generate the sympathetic	skin response in human chronic spinal cord injury
Authors: Nicotra Alessia , Catley Maria , Ellaway Peter H. , Mathias Christopher J. 
Published: December 2024

Purpose. Sympathetic sudomotor function in chronic spinal cord injury (SCI) has been evaluated to determine if recording the sympathetic skin response (SSR) provides evidence of integrity of the spinal component of the sympathetic pathways. Methods. Thirty subjects with chronic SCI and 15 healthy normal subjects were studied. The SSR was elicited using two physiological (auditory and inspiratory gasp) stimuli. In addition, electrical (median and peroneal nerve) stimulation was also performed. Recordings were made from palmar and plantar sites. Results. Palmar and plantar SSRs could be readily elicited in all control subjects by all stimuli. In the majority of SCI subjects, the presence or absence of the SSR was related to the American Spinal Injury Association (ASIA) impairment scale, which incorporates only motor and sensory assessments. The exceptions indicated preserved (or damaged) sympathetic spinal cord pathways. Conclusions. We conclude that the SSR, using either physiological or electrical stimuli, may be a reliable, non-invasive method of determining integrity of sympathetic cholinergic pathways in SCI, with potential for monitoring the effects of intervention and spinal repair.
High density of TCF1+ stem-like tumor-infiltrating lymphocytes is associated with favorable disease-specific survival in NSCLC
Authors: Førde Dagny , Kilvær Thomas , Pedersen Mona Irene , Blix Egil S , Urbarova Ilona , Paulsen Erna-Elise , Rakaee Mehrdad , Busund Lill-Tove Rasmussen , Donnem Tom , Andersen Sigve 
Published: December 2024

IntroductionTumor-infiltrating lymphocytes are both prognostic and predictive biomarkers for immunotherapy response. However, less is known about the survival benefits oftheir subpopulations.MethodsUsing machine learning models, we assessed the clinical association of the CD8+, PD1+, TCF1+ cel l subset by multiplex immunohistochemistry using tissue microarrays in 553 non-small cell lung cancer (NSCLC) patients and its correlation with other immune cell biomarkers.ResultsWe observed positive correlations between TCF1 and CD20 (r=0.37), CD3 (r=0.45)and CD4 (r=0.33). Notably, triple positive (CD8+PD1+TCF1+) were rare, only observed in 29 of 553 patients (5%). Our analysis revealed that cells coexpressing TCF1 with either CD8+ or PD1+ were independent prognostic markers of disease-specific survival in multivariable analysis (HR=0.728, p=0.029 for CD8+TCF1+, and HR=0.612, p=0.002 for PD1+TCF1+). To pilot the subtype of abundant CD8-TCF1+ cells, we explored an immune cell infiltrated whole slideimage and found the majority to be CD4+.DiscussionOverall, these findings suggest that assessment of CD8+, PD1+, TCF1+ could serve as a potential prognostic biomarker in NSCLC.
Mitogenomic analysis reveals the phylogenetic placement of monotypic Parachelon grandisquamis and distinctive structural features of control regions in mullets
Authors: Yoon Tae-Ho , Kang Hye-Eun , Aini Sarifah , Wujdi Arief , Kim Hyun-Woo , Kundu Shantanu 
Published: December 2024

IntroductionThe large-scale mullet, Parachelon grandisquamis (Teleostei: Mugilidae), is a monotypic species endemic to the eastern Atlantic Ocean, playing a crucial role in tropical ecosystems. Despite its ecological significance, the systematic classification of Mugilidae remains unresolved, largely due to their diverse morphology, which necessitates the integration of molecular data.MethodsThis study aimed to achieve a comprehensive molecular characterization of the species and establish its matrilineal taxonomic placement using complete mitogenome data. Next-generation sequencing was employed to generate the de novo mitogenome of P. grandisquamis, which spans 16,859 bp and includes 13 protein-coding genes (PCGs), 22 transfer RNAs, two ribosomal RNAs, and a non-coding AT-rich control region (CR).ResultsMost PCGs use ATG as the start codon, with the exception of COI, which begins with GTG. Analysis of amino acids abundance revealed high frequencies for leucine, serine, proline, threonine, and alanine with distinctive codon usage. The proportion of nonsynonymous and synonymous substitutions suggests strong purifying selection in most PCGs, except for ND4L, ND5, and ND6. Most transfer RNAs exhibited typical cloverleaf secondary structures, with the exception of tRNA-Ser1 (GCT), which lacks base pairing in the DHU arm. Mitogenome-based phylogenetic analysis using the Bayesian approach revealed that the monotypic P. grandisquamis is closely related to the genera Chelon and Planiliza within Mugilidae. Furthermore, analysis of the CRs with polymorphic nucleotides in conserved blocks provides additional insight into the development of distinct molecular markers for species identification and population structure analysis of mullets.DiscussionOverall, this study provides a comprehensive analysis of the mitogenomic structure and variation of P. grandisquamis and other mullets, confirming its maternal evolutionary relationships and offering valuable insights for advancing SNP-based species discrimination within the Mugilidae lineage.
The screening and anti-Raji lymphoma effect of Chiloscyllium plagiosum anti-CD20 VNARs
Authors: Ren Qingyu , Sheng Yu , Sun Ling , Zheng Feijian , Hu Chengwu , Chen Jianqing , Lyu Zhengbing , Yuan Chen , Jiang Xiaofeng 
Published: December 2024

IntroductionB-cell lymphoma, a malignant proliferative disease originating from lymphoid tissue, poses a grave threat to human health. CD20 has emerged as a promising target for lymphoma treatment. However, due to the significant heterogeneity of B-cell lymphomas, conventional CD20 monoclonal antibodies show limited penetration, severely impeding the progress of B-cell lymphoma therapies.MethodsIn contrast, single-domain antibody molecules derived from cartilaginous fish have a molecular weight as small as 12 kDa, granting them robust penetration capabilities and making them the smallest known molecules of efficiently targeting specific antigens.ResultsAs a result, these molecules hold tremendous potential as candidate drugs for lymphoma treatment. In this study, the whitespotted bamboo shark (Chiloscyllium plagiosum) was immunized with recombinant human CD20 to generate specific single-domain antibodies (sdAbs) targeting CD20. By utilizing phage display technology, the variable new antigen receptors (VNARs) were successfully screened and identified, and play an important role in the inhibition of Raji lymphoblastoma.DiscussionThe sdAbs obtained through this research represent promising candidates for B-cell lymphoma treatment, displaying significant potential for clinical applications and offering a new direction for the development of targeted therapies against lymphoma.
Development of 6-amido-4-aminoisoindolyn-1,3-diones as p70S6K1 inhibitors and potential breast cancer therapeutics
Authors: Thornton Adrian , Komati Rajesh , Kim Hogyoung , Myers Jamiah , Petty Kymmia , Sam Rion , Johnson-Henderson Elijah , Reese Keshunna , Tran Linh , Sridhar Vaniyambadi , Williams Christopher , Sridhar Jayalakshmi 
Published: December 2024

IntroductionMany breast cancer therapeutics target the PI3K/AKT/mTOR oncogenic pathway. Development of resistance to the therapeutics targeting this pathway is a frequent occurrence. Therapeutics targeting p70S6K1, a downstream member of this pathway, have recently gained importance due to its critical role in all types of breast cancer and its status as a prognostic marker. We have developed a new class of p70S6K1 inhibitors that show growth inhibition of MCF7 breast cancer cells.MethodsA series of 6-amido-4-aminoisoindolyn-1,3-dione compounds was developed against p70S6K1 using docking, computational modeling tools, and synthesis of the designed compounds. The p70S6K1 inhibition potency of the compounds was investigated in an initial high-throughput screening followed by IC50 determination for the most active ones. The best compounds were subjected to proliferation assays on MCF7 breast cancer cells. The targeting of p70S6K1 by the compounds was confirmed by studying the phosphorylation status of downstream protein rpS6.ResultsIn this study, we have identified a new class of compounds as p70S6K1 inhibitors that function as growth inhibitors of MCF7 breast cancer cells. The structural features imparting p70S6K1 inhibition potency to the compounds have been mapped. Our studies indicate that substitutions on the phenacetyl group residing in the cleft A of the protein do not contribute to the inhibition potency. Three compounds (5b, 5d, and 5f) have been identified to have sub-micromolar inhibition potency for p70S6K1. These compounds also exhibited growth inhibition of MCF7 cells by 40%–60% in the presence of estradiol.
Factors associated with cognitive flexibility in people with opioid-use disorder: a pilot study
Authors: Regier Paul S. , Macedo de Arruda Thais Costa , Sinko Laura , Teitelman Anne M. , Childress Anna Rose 
Published: December 2024

The ability to adapt to changing circumstances has strong survival value. Individuals with substance use disorders tend to get “stuck” over-responding to drug-reward signals and pursuing drugs despite negative consequences. A lack of flexibility may be tied to impairments in neurocognition, including learning, memory, and executive function. However, results are often mixed, potentially due to heterogeneity in factors such as mental health, personality traits, or prior adversity. This study aimed to identify which factors influence neurocognitive variations within the opioid use disorder (OUD) population. Based on prior literature, we hypothesized that individuals with OUD would show deficits (vs. controls) in one or more neurocognitive domains, and that these cognitive difficulties might be greater in individuals with other known contributors to impaired cognition. This pilot project included 32 individuals receiving medication for OUD and 15 non-substance using controls (NSC). Questionnaires assessed addiction and relapse risk factors, such as impulsiveness, social function, depressive symptoms, and childhood adversity. Neurocognitive performance was measured via the Penn Computerized Neurocognitive Battery (P-CNB), including tasks that probe attention, working memory, episodic memory, cognitive flexibility, and complex cognition, and was compared between the OUD and NSC groups. OUD participants (vs. NSCs) exhibited significantly lower performance on the conditional exclusion task (CET) (Accuracy: 1.11 vs. 2.38, p &lt; 0.001) and the n-Back task (NBT) (F1 Scores: 83% vs. 95%, p &lt; 0.001). Impulsiveness, social function, and depressive symptoms were highly inter-related; however, only higher impulsiveness (r = -.48, p = 0.006) and more social impairment (r = -.47, p = 0.007) significantly correlated with decreased CET (but not n-Back) performance. This pilot study suggests that working memory and cognitive flexibility are impaired in people with OUD and that impulsiveness and social function are key factors in cognitive flexibility impairments in people with OUD. These results may offer insights for larger-scale investigations and potential interventions to reduce relapse risk.