bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cyanobacterial multi-copy chromosomes and their replication
Authors: Watanabe Satoru 
Published: March 2020

AbstractWhile the model bacteria Escherichia coli and Bacillus subtilis harbor single chromosomes, which is known as monoploidy, some freshwater cyanobacteria contain multiple chromosome copies per cell throughout their cell cycle, which is known as polyploidy. In the model cyanobacteria Synechococcus elongatus PCC 7942 and Synechocystis sp. PCC 6803, chromosome copy number (ploidy) is regulated in response to growth phase and environmental factors. In S. elongatus 7942, chromosome replication is asynchronous both among cells and chromosomes. Comparative analysis of S. elongatus 7942 and S. sp. 6803 revealed a variety of DNA replication mechanisms. In this review, the current knowledge of ploidy and DNA replication mechanisms in cyanobacteria is summarized together with information on the features common with plant chloroplasts. It is worth noting that the occurrence of polyploidy and its regulation are correlated with certain cyanobacterial lifestyles and are shared between some cyanobacteria and chloroplasts.AbbreviationsNGS: next-generation sequencing; Repli-seq: replication sequencing; BrdU: 5-bromo-2′-deoxyuridine; TK: thymidine kinase; GCSI: GC skew index; PET: photosynthetic electron transport; RET: respiration electron transport; Cyt b6f complex: cytochrome b6f complex; PQ: plastoquinone; PC: plastocyanin.
Non-invasive characterisation of a laser-driven positron beam
Authors: Alejo A , Samarin G M , Warwick J , McCluskey C , Cantono G , Ceccotti T , Dobosz Dufrénoy S , Monot P , Sarri G 
Published: March 2020

Abstract
We report on an indirect and non-invasive method to simultaneously characterise the energy-dependent emittance and source size of ultra-relativistic positron beams generated during the propagation of a laser-wakefield accelerated electron beam through a high-Z converter target. The strong correlation of the geometrical emittance of the positrons with that of the scattered electrons allows the former to be inferred, with high accuracy, from monitoring the latter. The technique has been tested in a proof-of-principle experiment where, for 100 MeV positrons, we infer geometrical emittances and source sizes of the order of

ϵ

e
+

≈

3 µm and

D

e
+

≈

150 µm, respectively. This is consistent with the numerically predicted possibility of achieving sub-µm geometrical emittances and micron-scale source sizes at the GeV level.
Bistability and oscillations in cooperative microtubule and kinetochore dynamics in the mitotic spindle
Authors: Schwietert Felix , Kierfeld Jan 
Published: March 2020

Abstract
In the mitotic spindle microtubules attach to kinetochores via catch bonds during metaphase, and microtubule depolymerization forces give rise to stochastic chromosome oscillations. We investigate the cooperative stochastic microtubule dynamics in spindle models consisting of ensembles of parallel microtubules, which attach to a kinetochore via elastic linkers. We include the dynamic instability of microtubules and forces on microtubules and kinetochores from elastic linkers. A one-sided model, where an external force acts on the kinetochore is solved analytically employing a mean-field approach based on Fokker–Planck equations. The solution establishes a bistable force–velocity relation of the microtubule ensemble in agreement with stochastic simulations. We derive constraints on linker stiffness and microtubule number for bistability. The bistable force–velocity relation of the one-sided spindle model gives rise to oscillations in the two-sided model, which can explain stochastic chromosome oscillations in metaphase (directional instability). We derive constraints on linker stiffness and microtubule number for metaphase chromosome oscillations. Including poleward microtubule flux into the model we can provide an explanation for the experimentally observed suppression of chromosome oscillations in cells with high poleward flux velocities. Chromosome oscillations persist in the presence of polar ejection forces, however, with a reduced amplitude and a phase shift between sister kinetochores. Moreover, polar ejection forces are necessary to align the chromosomes at the spindle equator and stabilize an alternating oscillation pattern of the two kinetochores. Finally, we modify the model such that microtubules can only exert tensile forces on the kinetochore resulting in a tug-of-war between the two microtubule ensembles. Then, induced microtubule catastrophes after reaching the kinetochore are necessary to stimulate oscillations. The model can reproduce experimental results for kinetochore oscillations in PtK1 cells quantitatively.
High-resolution (diffraction limited) single-shot multiplexed coded-aperture ptychography
Authors: Ilan Haham Gil , Peleg Or , Sidorenko Pavel , Cohen Oren 
Published: March 2020

Abstract
We propose and numerically explore a method that upgrades a conventional single-shot microscope into a single-shot ptychographic microscope, without impairing its optical performance. It is based on splitting the microscope’s intermediate image plane into multiple replicas, and detecting a set of their coded Fourier transform magnitudes, using a different sensor for each replica. To code each beam, it is transmitted through a distinct coding mask. A ptychographic phase-retrieval algorithm is used to reconstruct the imaged object’s complex-valued transmission function. The proposed method also enables ultrahigh-speed imaging by using a pulse-burst as illumination and applying a multi-state reconstruction algorithm. This approach paves the way to both single-frame and ultrahigh-speed, diffraction-limited microscopy of complex-valued objects.
Electromagnetically induced transparency metamaterial with strong toroidal dipole response
Authors: Shen Zhaoyang , Yang Helin , Huang Xiaojun , Xiang Tianyu , Wu Jiong , Zhou Yanfei , Yu Zetai 
Published: March 2020

Abstract
This paper designs a planar electromagnetically induced transparency (EIT) metamaterial, which comprises an asymmetric ellipse split resonance ring (AESRR) and cut wire (CW). The proposed EIT metamaterial works in the wide range of incident angles and has polarization-sensitive at two transmission dips. The frequency of transparency peak is 10.67 GHz and maintains a high quality-factor (180.84). By calculating the multipole’s radiated power, it can be found that the toroidal dipole response is enhanced, while the electric dipole response is suppressed at the transparency peak. Interestingly, this paper firstly uses the radiated power of electric dipole to elucidate the polarization sensitivity in two minimal transmissions. Meanwhile, the coupling mechanism of the EIT metamaterial is analyzed by the two-oscillator model and equivalent circuit.
Reserpine Substantially Lowers Blood Pressure in Patients With Refractory Hypertension: A Proof-of-Concept Study
Authors: Siddiqui Mohammed , Bhatt Hemal , Judd Eric K , Oparil Suzanne , Calhoun David A 
Published: March 2020

Abstract

BACKGROUND
Refractory hypertension (RfHTN), a phenotype of antihypertensive treatment failure, is defined as uncontrolled automated office blood pressure (AOBP) ≥130/80 mm Hg and awake ambulatory blood pressure (ABP) ≥130/80 mm Hg on ≥5 antihypertensive medications, including chlorthalidone and a mineralocorticoid receptor antagonist. Previous studies suggest that RfHTN is attributable to heightened sympathetic tone. The current study tested whether reserpine, a potent sympatholytic agent, lowers blood pressure (BP) in patients with RfHTN.

METHODS
Twenty-one out of 45 consecutive patients with suspected RfHTN were determined to be fully adherent with their antihypertensive regimen. Seven patients agreed to participate in the current clinical trial with reserpine and 6 patients completed the study. Other sympatholytic medications, such as clonidine or guanfacine, were tapered and discontinued before starting reserpine. Reserpine 0.1 mg daily was administered in an open-label fashion for 4 weeks. All patients were evaluated by AOBP and 24-hour ABP at baseline and after 4 weeks of treatment.

RESULTS
Reserpine lowered mean systolic and diastolic AOBP by 29.3 ± 22.2 and 22.0 ± 15.8 mm Hg, respectively. Mean 24-hour systolic and diastolic ABPs were reduced by 21.8 ± 13.4 and 15.3 ± 9.6 mm Hg, mean awake systolic and diastolic ABPs by 23.8 ± 11.8 and 17.8 ± 9.2 mm Hg, and mean asleep systolic and diastolic ABPs by 21.5 ± 11.4 and 13.7 ± 6.4 mm Hg, respectively.

CONCLUSIONS
Reserpine, a potent sympatholytic agent, lowers BP in patients whose BP remained uncontrolled on maximal antihypertensive therapy, lending support to the hypothesis that excess sympathetic output contributes importantly to the development of RfHTN.
Change in the Relationship Between Drinking Alcohol and Risk of Violence Among Adolescents and Young Adults: A Nationally Representative Longitudinal Study
Authors: Jones Roland M , Van Den Bree Marianne , Zammit Stanley , Taylor Pamela J 
Published: March 2020

Abstract

Aims
To quantify the relationship between alcohol and violence with increasing age.

Methods
Data were from The National Longitudinal Study of Adolescent to Adult Health (ADD Health) of 20,386 people representative of the US population. Mean age at the first wave of interviews was 16.2 years, with subsequent interviews mean of 1, 6.3 and 12.9 years later. We used random-effects models and predictive marginal effects of the association between varying quantities of alcohol consumption and violence while controlling for possible confounders.

Results
Violence was reported by 19.1% of participants at wave I but just 2.1% at wave IV. The random-effects model showed that consuming 1–4 drinks on each occasion was associated with a modest increase in risk of violence in both males (odds ratio (OR) 1.36, 95% CI 1.13–1.63) and females (OR 1.33, 95% CI 1.03–1.72). For consumption of five or more drinks on each occasion, the risk remained similar for females (OR 1.40 (0.99–1.97)) but increased considerably for males (OR 2.41 (1.96–2.95)). Predictive marginal effects models confirmed that violence rates decreased with age.

Conclusions
Alcohol is most strongly linked to violence among adolescents, so programmes for primary prevention of alcohol-related violence are best targeted towards this age group, particularly males who engage in heavy episodic drinking.
tugHall: a simulator of cancer-cell evolution based on the hallmarks of cancer and tumor-related genes
Authors: Nagornov Iurii S , Kato Mamoru 
Published: March 2020

Abstract

Summary
The flood of recent cancer genomic data requires a coherent model that can sort out the findings to systematically explain clonal evolution and the resultant intra-tumor heterogeneity (ITH). Here, we present a new mathematical model designed to computationally simulate the evolution of cancer cells. The model connects the well-known hallmarks of cancer with the specific mutational states of tumor-related genes. The cell behavior phenotypes are stochastically determined, and the hallmarks probabilistically interfere with the phenotypic probabilities. In turn, the hallmark variables depend on the mutational states of tumor-related genes. Thus, our software can deepen our understanding of cancer-cell evolution and generation of ITH.

Availability and implementation
The open-source code is available in the repository https://github.com/nagornovys/Cancer_cell_evolution.

Contact
mamkato@ncc.go.jp

Supplementary information
Supplementary data are available at Bioinformatics online.
‘I teach them that anything is possible’—exploring how adult leaders perceive and handle social factors of youth mental health in the context of young people’s civic engagement
Authors: Gotfredsen Anne C , Landstedt Evelina 
Published: March 2020

AbstractThe aim of this qualitative study was 2-fold: to explore, in the context of young people’s civic engagement in Sweden, (i) how adult leaders perceive social factors of youth mental health and (ii) how adult leaders handle such social factors within their organizations. Interviews were conducted with leaders engaged in various civic organizations that provide leisure activities for young people. Using thematic analysis, three themes were constructed. Firstly, the social landscape of youth mental health described how adult leaders perceived the social factors of youth mental health within the context of civic engagement. Secondly, the organizational structures developed by adult leaders illustrated the organizing forms that leaders created for young people’s civic engagement. Thirdly, adult leaders’ strategies for addressing the social factors of youth mental health reflected the strategies developed to handle e.g. stress and achievement pressure. The adult leaders recognized the importance of their organizations and their huge potential to have a positive impact on youth mental health. However, some participants also saw limitations in terms of their own resources and competence. They found themselves having to address the complex issue of social factors of youth mental health regardless of whether they felt competent and prepared to do this or not. Our findings contribute to the existing knowledge on youth and community development via the role of adult leaders in promoting young people’s mental health by highlighting the organizational structures and leadership strategies developed by them.
Reward-Related Suppression of Neural Activity in Macaque Visual Area V4
Authors: Shapcott Katharine A , Schmiedt Joscha T , Kouroupaki Kleopatra , Kienitz Ricardo , Lazar Andreea , Singer Wolf , Schmid Michael C 
Published: March 2020

Abstract
In order for organisms to survive, they need to detect rewarding stimuli, for example, food or a mate, in a complex environment with many competing stimuli. These rewarding stimuli should be detected even if they are nonsalient or irrelevant to the current goal. The value-driven theory of attentional selection proposes that this detection takes place through reward-associated stimuli automatically engaging attentional mechanisms. But how this is achieved in the brain is not very well understood. Here, we investigate the effect of differential reward on the multiunit activity in visual area V4 of monkeys performing a perceptual judgment task. Surprisingly, instead of finding reward-related increases in neural responses to the perceptual target, we observed a large suppression at the onset of the reward indicating cues. Therefore, while previous research showed that reward increases neural activity, here we report a decrease. More suppression was caused by cues associated with higher reward than with lower reward, although neither cue was informative about the perceptually correct choice. This finding of reward-associated neural suppression further highlights normalization as a general cortical mechanism and is consistent with predictions of the value-driven attention theory.
A Case Report of Neonatal 2019 Coronavirus Disease in China
Authors: Wang Shaoshuai , Guo Lili , Chen Ling , Liu Weiyong , Cao Yong , Zhang Jingyi , Feng Ling 
Published: March 2020

Abstract
In December 2019, the coronavirus disease (COVID-19) caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China and now has spread in many countries. Pregnant women are a population susceptible to COVID-19 and are more likely to have complications and even progress to severe illness. We report a case of neonatal COVID-19 in China with pharyngeal swabs testing positive by real-time reverse-transcription polymerase chain reaction assay 36 hours after birth. However, whether the case is a vertical transmission from mother to child remains to be confirmed.
Erythropoiesis stimulating agents are associated with serum fibroblast growth factor 23 metabolism in patients on hemodialysis
Authors: Honda Hirokazu , Tanaka Kenji , Michihata Tetsuo , Shibagaki Keigo , Yuza Toshitaka , Hirao Keiichi , Tomosugi Naohisa , Ganz Tomas , Higashimoto Yuichiro 
Published: March 2020

Abstract

Background
This study aimed to determine associations among short- and long-acting erythropoiesis stimulating agents (ESAs), changes in serum fibroblast growth factor 23 (FGF23) and biomarkers of iron metabolism.

Methods
Among 108 patients on hemodialysis (HD), 44 received every 2 weeks or monthly doses of continuous erythropoiesis receptor activator (CERA), 31 received weekly doses of darbepoetin-α, 24 received three doses per week of epoetin-β and 9 were not treated with an ESA. Intact and C-terminal FGF23 and transferrin saturation (TSAT), ferritin, erythroferrone and hepcidin 25 were measured in blood samples collected before the HD session at the end of the dialysis week (baseline, Day 0) and on Days 3, 5, 7 and 14 thereafter.

Results
Levels of ferritin, hepcidin 25 and erythroferrone as well as TSAT were significantly decreased or elevated in patients treated with CERA compared with other types of ESAs. Levels of C-terminal FGF23 increased in all groups during the observation period. Levels of intact FGF23 and ratios of intact FGF23 to C-terminal FGF23 gradually decreased between Days 3 and 7 in the CERA but not in the other groups. Multivariate models associated changes in hepcidin 25 and phosphate with those of intact FGF23.

Conclusion
The long-acting ESA CERA might influence levels of intact FGF23 by increasing FGF23 cleavage in patients on HD in association with prolonged hepcidin 25 suppression.
Urinary soluble CD163 as a biomarker of disease activity and relapse in antineutrophil cytoplasm antibody-associated glomerulonephritis
Authors: Villacorta Javier , Lucientes Laura , Goicoechea Elena , Acevedo Mercedes , Cavero Teresa , Sanchez-Camara Luis , Díaz-Crespo Francisco , Gimenez-Moyano Sara , García-Bermejo Laura , Fernandez-Juarez Gema 
Published: March 2020

AbstractBackgroundAntineutrophil cytoplasm antibody (ANCA)-associated vasculitis is a chronic relapsing and remitting autoimmune disease. Urinary soluble CD163 (usCD163) has been proposed as a biomarker of active renal vasculitis. We aimed to assess the potential usefulness of usCD163 for diagnosing renal relapse in patients with ANCA-associated glomerulonephritis.MethodsOne hundred and fifty-six samples from 47 patients with ANCA-associated glomerulonephritis belonging to two different cohorts (incident and prevalent) and 20 healthy controls were studied. Patients from the incident cohort were prospectively followed up, and usCD163 concentrations were measured every 3 months. Renal relapses were identified and changes in usCD163 concentrations were analysed.ResultsNormalized usCD163 concentrations were elevated at disease onset in all patients with active renal vasculitis, with a median concentration of 601 ng/mmol (interquartile range 221–1404 ng/mmol). On the other hand, usCD163 concentrations were undetectable among control patients with renal vasculitis in remission. Except for non-responders, usCD163 concentrations progressively decreased in all patients after treatment. In the presence of vasculitis relapse, there was a consistent increase in usCD163 concentrations, compared with previous values. The area under the receiver-operating characteristic curve of absolute and relative changes in usCD163 concentrations to identify relapse of ANCA-associated glomerulonephritis was 0.96 [95% confidence interval (CI) 0.91–1.00; P = 0.001] and 0.95 (95% CI 0.90–1.00; P = 0.001), respectively. Sensitivity and specificity for a relative increase of 20%, or an absolute increase of 20 ng/mmol, in usCD163 concentrations were 100% for both, and 89.3% and 87.5%, respectively. Urinary sCD163 concentrations significantly correlated with Birmingham Vasculitis Activity Score scores at Month 6 (r = 0.737; P = 0.006) and Month 12 (r = 0.804; P = 0.005).ConclusionsusCD163 represents an accurate biomarker for the detection of active renal vasculitis and relapse. Its close association with disease activity provides additional information for monitoring treatment response.
Natriuretic peptide analogues with distinct vasodilatory or renal activity: integrated effects in health and experimental heart failure
Authors: Rademaker Miriam T , Scott Nicola J A , Koh Cho Yeow , Kini R Manjunatha , Richards A Mark 
Published: March 2020

Abstract

Aims
Management of acute decompensated heart failure (ADHF) requires disparate treatments depending on the state of systemic/peripheral perfusion and the presence/absence of expanded body–fluid volumes. There is an unmet need for therapeutics that differentially treat each aspect. Atrial natriuretic peptide (ANP) plays an important role in blood pressure and volume regulation. We investigate for the first time the integrated haemodynamic, endocrine and renal effects of human ANP analogues, modified for exclusive vasodilatory (ANP-DRD) or diuretic (ANP-DGD) activities, in normal health and experimental ADHF.

Methods and results
We compared the effects of incremental infusions of ANP analogues ANP-DRD and ANP-DGD with native ANP, in normal (n = 8) and ADHF (n = 8) sheep. ANP-DRD administration increased plasma cyclic guanosine monophosphate (cGMP) in association with dose-dependent reductions in arterial pressure in normal and heart failure (HF) sheep similarly to ANP responses. In contrast to ANP, which in HF produced a diuresis/natriuresis, this analogue was without significant renal effect. Conversely, ANP-DGD induced marked stepwise increases in urinary cGMP, urine volume, and sodium excretion in HF comparable to ANP, but without accompanying vasodilatory effects. All peptides increased packed cell volume relative to control in both states, and in HF, decreased left atrial pressure. In response to ANP-DRD-induced blood pressure reductions, plasma renin activity rose compared to control only during the high dose in normals, and not at all in HF—suggesting relative renin inhibition, with no increase in aldosterone in either state, whereas renin and aldosterone were both significantly reduced by ANP-DGD in HF.

Conclusion
These ANP analogues exhibit distinct vasodilatory (ANP-DRD) and diuretic/natriuretic (ANP-DGD) activities, and therefore have the potential to provide precision therapy for ADHF patients with differing pathophysiological derangement of pressure–volume homeostasis.