bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proofreading deficiency in mitochondrial DNA polymerase does not affect total dNTP pools in mouse embryos
Authors: Sharma Sushma , Koolmeister Camilla , Tran Phong , Nilsson Anna Karin , Larsson Nils-Göran , Chabes Andrei 
Published: March 2020

AbstractMitochondrial DNA (mtDNA) mutator mice express proofreading-deficient mtDNA polymerase gamma (PolgD257A) and age prematurely1,2, whereas mice with other mitochondrial defects do not show global signs of early aging. The reason for this discrepancy is not completely understood. Hämäläinenet al.recently reported that both induced pluripotent stem cells (iPSCs) and primary mutator embryonic fibroblasts harboring PolgD257Ademonstrate increased mtDNA replication frequency leading to depletion of the total cellular dNTP pool3. The authors proposed that the decreased availability of cellular dNTPs for nuclear genome replication leads to compromised nuclear genome maintenance and premature aging. Here we report that total cellular dNTP pools are normal in mtDNA mutator mouse embryos (genotype:PolgD257A/D257A), which shows that a living organism exclusively expressing PolgD257Ahas normal total dNTP pools despite ubiquitous rapid cell division.
Cargo crowding drives sorting stringency in COPII vesicles
Authors: Gomez-Navarro Natalia , Melero Alejandro , Boulanger Jerome , Kukulski Wanda , Miller Elizabeth A. 
Published: March 2020

AbstractAccurate maintenance of organelle identity in the secretory pathway relies on retention and retrieval of resident proteins. In the endoplasmic reticulum (ER), secretory proteins are packaged into COPII vesicles that largely exclude ER residents and misfolded proteins by mechanisms that remain unresolved. Here we combined biochemistry and genetics with correlative light and electron microscopy (CLEM) to explore how selectivity is achieved. Our data suggest that vesicle occupancy dictates ER retention: in the absence of abundant cargo, non-specific bulk flow increases. We demonstrate that ER leakage is influenced by vesicle size and cargo occupancy: overexpressing an inert cargo protein, or reducing vesicle size restores sorting stringency. We propose that cargo recruitment into vesicles creates lumenal steric pressure that drives selectivity. Sorting stringency is thus an emergent property of the biophysical process of cargo enrichment into a constrained spherical membrane-bound carrier.SummaryCombining correlative light and electron microscopy with yeast genetics and biochemistry, Gomez-Navarro, Melero and colleagues show that cargo recruitment into a constrained COPII vesicle restricts bulk flow, thereby contributing to sorting stringency and ER quality control.
Global gut content data synthesis and phylogeny delineate reef fish trophic guilds
Authors: Parravicini Valeriano , Casey Jordan M. , Schiettekatte Nina M. D. , Brandl Simon J. , Pozas-Schacre Chloé , Carlot Jérémy , Edgar Graham J. , Graham Nicholas A. J. , Harmelin-Vivien Mireille , Kulbicki Michel , Strona Giovanni , Stuart-Smith Rick D. , Vii Jason 
Published: March 2020

AbstractThe diversity of life on our planet has produced a remarkable variety of biological traits that characterize different species. Such traits are widely employed instead of taxonomy to increase our understanding of biodiversity and ecosystem functioning. However, for species’ trophic niches, one of the most critical aspects of organismal ecology, a paucity of empirical information has led to inconsistent definitions of trophic guilds based on expert opinion. Using coral reef fishes as a model, we show that experts often disagree on the assignment of trophic guilds for the same species. Even when broad categories are assigned, 60% of the evaluated trait schemes disagree on the attribution of trophic categories for at least 20% of the species. This disagreement greatly hampers comparability across studies. Here, we introduce a quantitative, unbiased, and fully reproducible framework to define species’ trophic guilds based on empirical data. First, we synthesize data from community-wide visual gut content analysis of tropical coral reef fishes, resulting in trophic information from 13,961 individuals belonging to 615 reef fish species across all ocean basins. We then use network analysis to cluster the resulting global bipartite food web into distinct trophic guilds, resulting in eight trophic guilds, and employ a Bayesian phylogenetic model to predict trophic guilds based on phylogeny and maximum body size. Our model achieved a misclassification error of 5%, indicating that our approach results in a quantitative and reproducible trophic categorization scheme, which can be updated as new information becomes available. Although our case study is for reef fishes, the most diverse vertebrate consumer group, our approach can be applied to other organismal groups to advance reproducibility in trait-based ecology. As such, our work provides an empirical and conceptual advancement for trait-based ecology and a viable approach to monitor ecosystem functioning in our changing world.
Low frequency traveling waves in the human cortex coordinate neural activity across spatial scales
Authors: Sreekumar Vishnu , Wittig John H. , Chapeton Julio I. , Inati Sara K. , Zaghloul Kareem A. 
Published: March 2020

AbstractTraveling waves of oscillatory activity are thought to influence neuronal spiking, providing a spatiotemporal frame-work for neural communication. However, no direct link has been established between traveling waves and neuronal spiking in humans. We examined traveling waves in the human lateral temporal lobe by using recordings from intracranial electrodes implanted in twenty participants for seizure monitoring as they performed a paired-associates verbal memory task. We observed ubiquitous low frequency traveling waves across the temporal lobe. While wave occurrence in a broad low frequency range did not differ between successful and unsuccessful memory conditions, in a subset of participants with microelectrode recordings, we found that macro-scale waves co-occurred with micro-scale waves, which in turn were temporally locked to single unit spiking. This temporal coordination between traveling waves at different spatial scales and between waves and neuronal spiking in the human brain suggests a role for traveling waves in neural communication.
Germplasm of Brazilian winter squash (Cucurbita moschataD.) displays vast genetic variability, allowing identification of promising genotypes for agro-morphological traits
Authors: Gomes Ronaldo Silva , Júnior Ronaldo Machado , de Almeida Cleverson Freitas , Chagas Rafael Ravaneli , de Oliveira Rebeca Lourenço , Delazari Fabio Teixeira , da Silva Derly José Henriques 
Published: March 2020

ABSTRACTWinter squash fruits (Cucurbita moschataD.) are among the best sources of vitamin A precursors and constitute sources of bioactive components such as phenolic compounds and flavonoids. Approximately 70% ofC. moschataseed oil is made up of unsaturated fatty acids, with high levels of monounsaturated fatty acids and components such as vitamin E and carotenoids, which represent a promising nutritional aspect in the production of this vegetable.C. moschatagermplasm expresses high genetic variability, especially in Brazil. We assessed 91C. moschataaccessions, from different regions of Brazil, and maintained at the UFV Vegetable Germplasm Bank, to identify early-flowering accessions with high levels of carotenoids in the fruit pulp and high yields of seed and seed oil. Results showed that the accessions have high variability in the number and mass of seeds per fruit, number of accumulated degree-days for flowering, total carotenoid content, and fruit productivity, which allowed selection for considerable gains in these characteristics. Analysis of the correlation between these characteristics provided information that will assist in selection to improve this crop. Cluster analysis resulted in the formation of 16 groups, confirming the variability of the accessions.Per seanalysis identified accessions BGH-6749, BGH-5639, and BGH-219 as those with the earliest flowering. Accessions BGH-5455A and BGH-5598A had the highest carotenoid content, with averages greater than 170.00 μg g-1of fresh mass. With a productivity of 0.13 t ha-1, accessions BGH-5485A, BGH-4610A, and BGH-5472A were the most promising for seed oil production. These last two accessions corresponded to those with higher seed productivity, averaging 0.58 and 0.54 t ha-1, respectively. This study confirms the high potential of this germplasm for use in breeding for promotion of earlier flowering and increase in total carotenoid content of the fruit pulp and in seed and seed oil productivity.
Effect of stretch goals on work–family conflict
Authors: Chen Chang , Zhang Zhe , Jia Ming 
Published: March 2020

PurposeThe purpose of this paper is to examine the destructive effects of stretch goals on employees’ work–family conflict (WFC). Drawing on the conservation of resources (COR) theory, this study examines the mediating role of resource scarcity. By integrating the paradox theory with the COR theory, this study explores the moderating role of employees’ paradox mind-set.Design/methodology/approachTwo-wave data were collected from a sample of MBA students in Northwestern China (N= 294). PROCESS was used to assess a moderated mediation model.FindingsThis study found a positive relationship between stretch goals and WFC, and resource scarcity mediated this relationship. For employees with a high paradox mind-set, the relationship between resource scarcity and WFC was weak; and the indirect effect of stretch goals on WFC via resource scarcity was weak.Practical implicationsOrganizations should provide enough resources to employees when using stretch goals. Human resource managers could recruit candidates with high paradox mind-set and foster employees’ paradox mind-set through training.Originality/valueThis study makes contributions to the literature on stretch goals by examining the negative spillover effect of stretch goals on the family domain and exploring the mediating mechanism. This study also extends the paradox theory by using it at micro level to address questions on WFC.
Store brand and perceived risk on private label brand attitude
Authors: Manikandan M.K.M. 
Published: March 2020

Purpose
The purpose of this paper is to find the influence of retailer equity and perceived risk on attitudes toward private label brand (PLB) grocery products.

Design/methodology/approach
Retailer equity includes four variables: retailer awareness, retailer association, retailer perceived quality and retailer loyalty. The perceived risk factors include functional risk, financial risk and social risk. The attitude toward PLBs was taken as the dependent variable. The study was carried out by using a standardized questionnaire for all three constructs. The convenience sampling method was adopted to carry out data collection from customers of organized retail stores in the city of Coimbatore, in the state of Tamil Nadu, India. The relationship between the three variables was studied with structural equation modeling using IBM SPSS Amos software.

Findings
The study revealed that excluding the Financial Risk and the Social Risk, functional risk alone has significant influence over the PLB Attitude. The Retailer Equity variables, retailer perceived quality and retailer loyalty have positive influence on the PLB Attitude, while the other two variables do not show any influence. Retailer Awareness shows a negative influence over the social risk. Retailer Association does not show any influence on any of the three risk factors. Retailer perceived quality shows negative influence over the functional risk while retailer loyalty negatively influences social risk.

Research limitations/implications
The research study was carried out in cities that are populous in the Indian state of Tamil Nadu. All the respondents came from three cities in Tamil Nadu, namely, Coimbatore, Tiruppur and Madurai. Hence, extending the findings of the study to other countries where organized retail penetration is deeper may be attempted with caution.

Practical implications
The study will offer managers in the retail industry some understanding of the risk-relieving factors in operation when buying grocery goods.

Originality/value
The research paper contributes to the literature concerning the role played by retailer equity and perceived risk factors on attitudes toward PLBs.
Culture and green supply chain management (GSCM)
Authors: Elbaz Jamal , Iddik Saadia 
Published: March 2020

PurposeThis paper seeks to explore, summarize, and interpret the existing literature linking green supply chain management (GSCM) to culture. To achieve this goal, three main questions are answered: (1) How culture has been linked to GSCM in prior works? (2) What are the general problems exposed? (3) What gaps to detect?Design/methodology/approachA systematic literature review related to culture–GSCM was conducted. The review combines descriptive and content analysis of 46 academic peer-reviewed English papers from 30 international journals.FindingsAround 90 percent of the articles are empirical papers, only 10 percent are conceptual papers. These papers systematically focused on two issues: the largest number deal with the organizational culture–environmental practices, then national culture–environmental practices. The GSCM–culture relationship underlines contrast findings stemmed from various factors, levels of analysis, different sample, research design, contexts, and so on. Among the results, a conceptual model is developed, allowing suggestions for future research.Research limitations/implicationsThis review is limited by the environmental practices of SCM raised in the literature, excluding corporate responsibility or ethics researches. This study may not be exhaustive and expose all papers in the field, but it does definitively assess a wider number of papers available and to which access is provided. Future researchers empirically test the conceptual model proposed toward more clarifications.Practical implicationsThis paper provides managers with an extra element to take into consideration while accustoming with GSCM, which is better understanding the role and the sensitivity of cultural values in order to harmonize with the changes.Originality/valueThis paper had extended the prior reviews to add recent and new papers. It does not only update the understanding related to culture–GSCM but goes beyond that to propose a multidisciplinary model theorizing the existing links and calling for ultimate investigations for the detected gaps.
Research on a non-linear calibration method for dynamometer
Authors: Jun Zhang , Qingbing Chang , Zongjin Ren 
Published: March 2020

Purpose
The purpose of this paper is to solve the problem that the relationship between loading forces, which were applied at different positions on a plane, and output values of load-sharing dynamometer is non-linear.

Design/methodology/approach
First, the analytical model of ISPM (isodynamic surface proportional mapping method) method, which is used to calibrate dynamometer, was established. Then, a series of axial force calibration tests were performed on a load-sharing dynamometer at different loading positions. Finally, according to output values, calibration forces at different loading positions were calculated by ISPM method, and corresponding distribution histogram of calibration force error was generated.

Findings
The largest error between calculated force and standard force is 2.92 per cent, and the probability of calculated force error within 1 per cent is 91.03 per cent, which verify that the ISPM method is reliable for non-linear calibration of dynamometers.

Originality/value
The proposed ISPM method can achieve non-linear calibration between measured force and output signal of load-sharing dynamometer at different positions. In addition, ISPM method can also solve some complex non-linear problems, such as prediction of plane cutting force under the influence of multiple parameters, the force measurement of multi-degree-of-freedom platform and so on.
Multi-objective optimization of wire electrical discharge machining of 20MnCr5 alloy steel
Authors: Kumar Naresh , Goyal Khushdeep 
Published: March 2020

PurposeWire electric discharge machining (WEDM) is a non-conventional machining process, which is used to provide difficult and intricate shapes. The purpose of this research work is to apply Taguchi’s technique to optimize the process parameters in WEDM. Alloy steel 20MnCr5 has been selected as base material for experimentation. The effects of the input process parameters such as wire type, pulse-on time, pulse-off time, peak current, wire feed rate and servo voltage have been calculated on the material removal rate (MRR) and surface roughness (Ra) in WEDM operation.Design/methodology/approachIn the research work, Taguchi's technique is applied to optimize the process parameters in WEDM.FindingsANOVA indicated that pulse-off time was the most significant factor for the MRR, and servo voltage was the most significant factor for surface roughness (SR). As a part of the project, 20MnCr5 was machined in wire electric discharge machine, and the optimal control parameters were found to get higher MRR and better SR using Taguchi’s technique.Originality/valueTo the best of authors’ knowledge, after reviewing the literature, materials including alloys of metals such as 16MnCr5 and 20MnCr5 have not been investigated so far, and research regarding machining of these materials is limited. Therefore, 20MnCr5 material has been selected for this research work to generate WEDM data.
Splitting the Tactical Vote? Coordination Problems with Polling Model‐Driven Tactical Voting Websites
Authors: Nicholls Tom , Hayton Richard 
Published: March 2020

AbstractThe 2019 general election gave prominence to tactical voting advice websites, particularly those projecting constituency‐level results using national polling data and sophisticated multi‐level regression and poststratification (MRP) polling models. We see tactical voting as an example of a coordination game and argue that polling‐model driven advice websites are disruptive to the existing focal points for strategic voting. Although such websites may well help identify the strongest candidate based on constituency demographics, the article argues they are unlikely to coalesce support behind that single candidate, a prerequisite for successful tactical voting. In practice, there are multiple competing models and websites as well as other key sources of information to voters. Furthermore, the heuristic of relying on tactical voting websites is vulnerable to strategic action by partisans. In short, it is argued that the emergence of these websites risks muddying the waters further for electors seeking to vote tactically, potentially splitting the tactical vote rather than unifying it.
Negotiating the ‘buffet’ of choice: advances in technology and end‐of‐life decision‐making in the intensive care unit setting
Authors: Bandini Julia I. 
Published: March 2020

AbstractIn recent years, increases in medical technologies in the critical care setting have advanced the practice of medicine, enabling patients to live longer while also creating dilemmas for end‐of‐life decision‐making. Clinicians have increasingly been called on to involve patients and family members in decision‐making through a process of shared decision‐making (SDM), yet less is known about howSDMplays out in the critical care setting and the ways in which clinicians engage inSDM. Using observational data from 14 months of ethnographic fieldwork in two intensive care units and interviews with 33 family members of 25 critically ill patients and 51 clinicians, I explore how clinicians refer to the choices available in medical decision‐making paradoxically as a ‘buffet’ of choice while they simultaneously recognise that such rhetoric is misaligned with complex and emotional decision‐making, often involving pain and suffering. Lastly, this paper considers the role ofSDMand the ways in which clinicians push back on the ‘buffet’ rhetoric and engage in practices to guide families in end‐of‐life decision‐making bygranting permissionfor families to make decisions andvalidatingtheir decisions to decline treatment when there is an opportunity for more treatment.
Standard guidelines for the publication of telomere qPCR results in evolutionary ecology
Authors: Morinha Francisco , Magalhães Paula , Blanco Guillermo 
Published: March 2020

AbstractTelomere length has been used as a proxy of fitness, aging and lifespan in vertebrates. In the last decade, dozens of articles reporting on telomere dynamics in the fields of ecology and evolution have been published for a wide range of taxa. With this growing interest, it is necessary to ensure the accuracy and reproducibility of telomere length measurement techniques. Real‐time quantitative PCR (qPCR) is routinely applied to measure relative telomere length. However, this technique is highly sensitive to several methodological variables and the optimization of qPCR telomere assays remains highly variable between studies. Therefore, standardized guidelines are required to enable the optimization of robust protocols, and to help in judging the validity of the presented results. This review provides an overview of preanalytical and analytical factors that can lead to qPCR inconsistencies and biases, including: (a) sample type, collection and storage; (b) DNA extraction, storage and quality; (c) qPCR primers, laboratory reagents, and assay conditions; and (d) data analysis. We propose a minimum level of information for publication of qPCR telomere assays in evolutionary ecology considering the methodological pitfalls and sources of error. This review highlights the complexity of the optimization and validation of qPCR for telomere measurement per se, demonstrating the importance of transparency and clarity of reporting methodological details required for reliable, reproducible and comparable qPCR telomere assays. We encourage efforts to implement standardized protocols that ensure the rigour and quality of telomere dynamics studies.
Dynamic competition with network externalities: how history matters
Authors: Halaburda Hanna , Jullien Bruno , Yehezkel Yaron 
Published: March 2020

AbstractWe consider dynamic competition among platforms in a market with network externalities. A platform that dominated the market in the previous period becomes “focal” in the current period, in that agents play the equilibrium in which they join the focal platform whenever such equilibrium exists. Yet when faced with higher‐quality competition, can a low‐quality platform remain focal? In the finite‐horizon case, the unique equilibrium is efficient for “patient” platforms; with an infinite time horizon, however, there are multiple equilibria where either the low‐ or high‐quality platform dominates. If qualities are stochastic, the platform with a better average quality wins with a higher probability, even when its realized quality is lower, and this probability increases as platforms become more patient. Hence, social welfare may decline as platforms become more forward looking.
Measuring competition in spatial retail
Authors: Ellickson Paul B. , Grieco Paul L.E. , Khvastunov Oleksii 
Published: March 2020

AbstractWe propose and estimate a spatially aggregated discrete‐choice model with overlapping consumer choice sets and demographic‐driven heterogeneity that varies by chain. Our approach avoids the need to define markets ex ante and captures rich substitution patterns, even in the absence of price data. An application to the US grocery industry illustrates the importance of location, format, and the spatial distribution of consumers in shaping the competitive environment. Contrary to conventional wisdom, we find substantial cross‐format competition between supercenters, clubs, and traditional grocers. Finally, we evaluate two representative mergers between supermarket chains to demonstrate how our estimates inform antitrust policy.