bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA MIR497HG suppresses bladder cancer progression through disrupting the crosstalk between Hippo/Yap and TGF-β/Smad signaling
Authors: Zhuang Changshui , Liu Ying , Yuan Chaobo , Fu Shengqiang , Yang Weifeng , Luo Jingwen , Zhuang Chengle 
Published: September 2020

Abstract
Objectives

A subclass of long non-coding RNAs (lncRNAs), categorized as miRNA-host gene lncRNAs (lnc-miRHGs), is processed to produce miRNAs and involve in cancer progression. This work aimed to investigate the influence and the molecular mechanisms of lnc-miRHGs MIR497HG in bladder cancer (BCa).
Materials and methods

The miR-497 and miR-195 were derived from MIR497HG. Cell proliferation, migration and invasion assays were used to measure the function of MIR497HG, miR-497 and miR-195 in BCa. Bioinformatics, RT-qPCR, western blot, luciferase reporter assay, ChIP, and so on, were used to reveal the upstream and downstream mechanisms of MIR497HG in BCa.
Results

We identified that lnc-miRHG MIR497HG and two harbored miRNAs, miR-497 and miR-195, were downregulated in BCa by analyzing TCGA and our dataset. MIR497HG overexpression inhibited BCa cell proliferation, migration and invasion in vitro. MiR-497/miR-195 inhibitor rescued significantly the inhibiton effects of overexpression of MIR497HG in BCa. Mechanistically, miR-497 and miR-195 coordinately suppressed multiple key components in Hippo/Yap and TGF-β signaling, and particularly attenuated the interaction between Yap and Smad3. In addition, E2F4 was proved to be critical for silencing MIR497HG transcription in BCa cells.
Conclusions

We propose for the first time that MIR497HG suppressed BCa progression and its upstream and downstream mechanisms. Blocking the pathological process may be a potential strategy for the treatment of BCa.
Corosolic Acid Inhibits Tumor Growth without Compromising ALPPS-induced Liver Regeneration in Rats
Authors: zhao Jinwei , luo xueyue , zhao weiyi , zhou hong , xu hongyue , wang xuefei , luan wenjing , an yanan , wang chao , Yu Lu 
Published: September 2020

Abstract
BackgroudThe associating liver partition and portal vein ligation (ALPPS) technique is a promising strategy for unresectable tumors without sufficient future liver remnants (FLRs). Criticism has focused on its stimulation of tumor growth. This study explores the effect of corosolic acid (CA) on inhibiting tumor growth without compromising ALPPS-induced liver regeneration and investigates its possible mechanism.MethodsThe ALPPS procedure was performed in Sprague-Dawley rats with orthotopic liver cancer. Blood, tumor and FLR samples in different group were collected.ResultsThe tumor weight in the implantation/ALPPS/CA group was lower than that in the implantation/ALPPS group (p < 0.05). On postoperative day 15, the hepatic regeneration rate and the expression of Ki67+ hepatocytes in the FLRs increased significantly in the group that underwent ALPPS. The number of CD86+ macrophages increased in the FLRs and tumors of the groups that underwent the ALPPS procedure. Additionally, the number of CD206+ macrophages was higher than the number of CD86+ macrophages in the tumors of the implantation group and the implantation/ALPPS group (p < 0.01, respectively); however, the opposite results were observed in the CA groups. The administration of CA downregulated the expression of TGF-β, CD31 and PD-1, whereas it increased the number of CD8+ lymphocytes in tumors.ConclusionsCA inhibits tumor growth without compromising ALPPS-induced liver regeneration. This result may be attributed to the CA-induced downregulation of PD-1 and TGF-β expression and the increased CD8+ lymphocyte infiltration in tumor tissue, associated with the suppression of M2 macrophage polarization.
Metabolic Engineering of Bacillus Amyloliquefaciens as A Novel Cell Factory to Produce Spermidine
Authors: Zou Dian , Li Lu , Min Yu , Ji Anying , Liu Yingli , Wei Xuetuan , Wang Jing , Wen Zhiyou 
Published: September 2020

Abstract
Background: Spermidine is a biologically active polyamine with extensive application potential in foods and pharmaceuticals. However, previously reported spermidine titers by biosynthesis methods are relatively low, which hinders the industrial fermentation of spermidine. To improve the spermidine titer, key genes affecting the spermidine production were mined to engineer the Bacillus amyloliquefaciens.Results: Genes of S-adenosylmethionine decarboxylase (speD) and spermidine synthase (speE) from different microorganisms were expressed and compared in B. amyloliquefaciens. Therein, the speD from Escherichia coli and speE from Saccharomyces cerevisiae were confirmed to be optimal for spermidine synthesis, respectively. Then, these two genes were co-expressed to generate an engineering strain B. amyloliquefaciens HSAM2(PDspeD-SspeE) with a spermidine titer of 91.31 mg/L, improving by 10.90-fold compared with the control (HSAM2). Through further optimization of fermentation medium, the spermidine titer was increased to 227.35 mg/L, which was the highest titer among present reports. Moreover, the consumption of the substrate S-adenosylmethionine was consistent with the accumulation of spermidine, which contributed to understanding the synthetic pattern of spermidine. Conclusions: Two critical genes for spermidine synthesis were obtained, and an B. amyloliquefaciens cell factory was constructed for enhanced spermidine production, which laid the foundation for further industrial production of spermidine.
COVID-19 ICU and Mechanical Ventilation Patient Characteristics and Outcomes - A Systematic Review and Meta-analysis
Authors: Chang Raymond , Elhusseiny Khaled Mossad , Yeh Yu-Chang , Sun Wei-Zen 
Published: September 2020

Abstract
Background To characterize COVID-19 ICU admissions, their outcome and associated features, as well as identify their regional discrepancies. Methods Scopus, Embase, preprint servers bioRvix and medRvix and the Intensive Care National Audit and Research Center (ICNARC) website was searched for reports through May 1st 2020 on COVID-19 ICU admissions and outcomes using pre-defined search terms and eligibility criteria.Relevant data was extracted and pooled using fixed or random effects meta-analysis depending on heterogeneity. Study quality was assessed by the NIH tool and heterogeneity was assessed by I2 and Q tests. Baseline patient characteristics, ICU and IMV outcomes were pooled and meta-analyzed. Pooled odds ratios (pOR) were calculated for clinical features against ICU, MV mortality. Subgroup analysis was carried out based on patient regions.Results Twenty-eight studies comprising 12,437 COVID-19 ICU admissions from seven countries were meta-analyzed. Pooled ICU admission rate was 21%[95% CI 0.12 to 0.34] and 69% of cases needed IMV[95% CI 0.61-0.75]. ICU and IMV mortality were 28.3%[95% CI 0.25 to 0.32], 43%[95% CI 0.29 to 0.58] and ICU, IMV duration was 7.78[95% CI 6.99 to 8.63] and 10.12[95% CI 7.08 to 13.16] days respectively. Besides confirming the significance of comorbidities and clinical findings of COVID-19, major correlates with ICU mortality were found to be IMV [pOR 16.46, 95% CI 4.37 to 61.96], acute kidney injury (AKI) [pOR 12.47, 95% CI 1.52 to 102.7], and acute respiratory distress syndrome (ARDS) [pOR 6.52, 95% CI 2.66 to 16.01]. Subgroup analyses confirm significant regional discrepancies in outcomes.Conclusions This is the most comprehensive systematic review and meta-analysis of COVID-19 ICU and IMV cases and associated outcomes to date and the only analysis to analyze and associate IMV with COVID-19 ICU mortality. The significant association of AKI, ARDS and IMV with mortality has implications for ICU resource planning for AKI and ARDS as well as research into optimal ventilation strategies for patients. Regional differences in outcome implies a need to develop region specific protocols for ventilatory support as well as overall treatment.Study Registration PROSPERO registration number CRD42020182482.
PTP4A2 regulates Sorafenib resistance via activating autophagy in hepatocellular carcinoma
Authors: Chen Danlei , He Ying , Zhou Mi , Gao Ying , Zheng Zhou-San , Ye Wen , Xie Dan , Zhang Jiaxing , Ye Sheng 
Published: September 2020

Abstract
Background: To investigate the function and the potential mechanism of PTP4A2 in the progression of hepatocellular carcinoma (HCC). Method: We analyzed PTP4A2 expression in patients with HCC and evaluated the relevance with clinical features. To investigate the role of PTP4A2 on the proliferation, apoptosis, autophagy and sorafenib sensitivity of HCC cells. Then miRNA's targeted regulation mechanism for PTP4A2 and the downstream target mechanism were explored. Results: Here, we found that PTP4A2 expression increased significantly in HCC tissues, and report that PTP4A2 is an independent prognostic factor fo HCC patients in the first time. Silencing of PTP4A2 decreased cell proliferation and autophagy, and increased cell apoptosis and sorafenib chemosensitivity, while the effect of PTP4A2 overexpression was opposite. In addition, miR-425 was found to negatively regulate PTP4A2. Mechanismly, the upregulation of PTP4A2 activated autophagy by targeting the RAB24. Overexpression of RAB24 re-enhances PTP4A2-silenced HCC cells proliferation and autophagy. Upregulation of RAB24 by PTP4A2 is partially dependent on PTP4A2 promotes HDAC1 accumulation in the cytoplasm of HCC cells and epigenetically up-regulates RAB24 transcription. Conclusion: This study confirmed that PTP4A2 is an oncogene in HCC, and antagonizing PTP4A2 may be a potential strategy of treatment to increase HCC cells’ Sorafenib sensitivity.
MiR-124 and Small Molecules Synergistically Regulate the Direct Generation of Neuronal Cells from Rat Cortical Reactive Astrocytes
Authors: Zheng Yangyang , Huang Zhehao , Xu Jinying , Hou Kun , Yu Yifei , Lv Shuang , Chen Lin , Li Yulin , Quan Chengshi , Chi Guangfan 
Published: September 2020

Abstract
Background：Irreversible neuron loss caused by central nervous system injuries usually lead to persistent neurological dysfunction. Reactive astrocytes, because of their high proliferative capacity, proximity to neuronal lineage, and significant involvement in glial scarring, are ideal starting cells for neuronal regeneration. Having previously identified several small molecules as important regulators of astrocyte-to-neuron reprogramming, our aim in this study was to explore whether other small molecules and miR-124, a key neural differentiation mediator, could co-regulate reactive astrocyte-to-neuron conversion.Methods: MiR-124, ruxolitinib, SB203580, and forskolin were used to induce postnatal rat cortex reactive astrocytes, and the neuronal phenotype of the induced cells was characterised. To understand the genetic changes, RNA-sequencing analyses were performed on reactive astrocytes, induced neurons, and rat neurons, and the mechanisms underlying the regulatory role of miR-124 during the neuronal conversion was explored.Results：MiR-124, ruxolitinib, SB203580, and forskolin could co-convert rat cortical reactive astrocytes into neurons. The induced cells had reduced astroglial properties, displayed typical neuronal morphologies, and expressed neuronal markers, reflecting 25.9% of cholinergic neurons and 22.3% of glutamatergic neurons. Gene analysis revealed that induced neuron gene expression patterns were more similar to that of primary neurons than of initial reactive astrocytes. On the molecular level, miR-124-driven neuronal differentiation of reactive astrocytes was via targeting of the SOX9-NFIA-HES1 axis to inhibit HES1 expression.Conclusions：Providing a novel approach for inducing endogenous rat cortical reactive astrocytes into neurons by co-regulation involving miR-124 and three small molecules, our research has potential implications for inhibiting glial scar formation and promoting neuronal regeneration after central nervous system injury or disease.
The Epidemiological and Spatiotemporal Characteristics of 2019 novel coronavirus diseases (COVID-19) in Libya
Authors: Daw Mohamed A , El-Bouzedi Abdallah H , Ahmed Mohamed O 
Published: September 2020

Abstract
Background: COVID-19 is a global pandemic that affectedaffects all aspects of life. Studies on understanding the geographical and epidemiological characteristics become particularly important in controlling the spread of the pandemic. Such studies are lacking in Northern African countries, particularly in Libya, which is considered being, the second largest country in Africa, with the longest coast facing Europe. The objectives of this study are to; determine the epidemiological parameters and spatiotemporal patterns of COVID-19 in this large country and outline the needed strategies to contain the spread and the consequences of the pandemic. Methods: This comprehensive study included all the confirmed cases of COVID-19 since its emergence in Libya, from early April 2020 until July 31th31. The epidemiological characteristics of COVID-19 were analyzed, and the spatial dynamic trends were explored. A region Region-based counts of weekly reported cases were used to characterize and quantify the spatial dynamics of COVID-19.Results: A total of 3695 of confirmed cases of OVID-19 were collected,; 2515 (68.1%) were males , and 1180 (31.9%) were females with a male-to-female (M:F) ratio of 2.1:1. Aged between 2 -and 78 years old. Older age patients infected with COVID-19 are at higher severity and mortality. A broad geographic variability and spatiotemporal spread variation of the COVID-19 pandemic in Libya was observed. Indicating. This indicates a more significant increase ofin COVID-19 from the middle of July, particularly in the West and Southern regions, although it was consistently observed in the Meddle and Southern regions.Conclusion: Evaluating the epidemiological data and the spatiotemporal dynamic trends of COVID-19 at early stages are particularly important in understanding the pandemic spread. These parameters are essential in designing effective prevention and control programs aimed at reducing the impact of the COVID- 19 pandemic, particularly in countries with limited resources.
The Effectiveness of Ramosetron and Ondansetron for Preventing Postoperative Nausea and Vomiting After Arthroscopic Rotator Cuff Repair: a Randomized Controlled Trial
Authors: Kim Yang-Soo , Lee Hyo-Jin , Lee Sang-Uk 
Published: September 2020

Abstract
BackgroundArthroscopic rotator cuff repair is a painful procedure, and treatment of emetic events associated with drugs used in the current multimodal pain management remains challenging. This study aimed to evaluate the effectiveness of ramosetron or ondansetron to relieve PONV and pain after arthroscopic rotator cuff repair.MethodsIn total, 122 consecutive patients undergoing arthroscopic rotator cuff repair were randomly allocated into three groups: ramosetron group (n=39), ondansetron group (n=43), and Control group (n=40). Then, 0.3 mg of ramosetron or 8 mg of ondansetron was administered intravenously at the end of surgery according to group. All patients received general anesthesia and multimodal pain management protocol including preemptive analgesic medication, fentanyl-based intravenous patient-controlled analgesia, and postoperative analgesic medication. Incidence of emetic events, rescue antiemetic requirements (10 mg of metoclopramide, IV), complete response, pain level, and side effects were recorded in three periods: 0–6, 6–24, and 24–48 h postoperatively. The severity of nausea and pain was evaluated using visual analogue scale.ResultsThe ramosetron group tended to have a lower incidence and severity of nausea during the 6- to 24-h postoperative period and fewer rescue antiemetic drug requirements during the 0- to 48-h period than the control group, showing statistical significance (p<0.05). Additionally, the frequency of complete response of the ramosetron and ondansetron groups was significantly higher than that of the Control group. No difference was found among the groups in the pain level except during the 0- to 6-h period. The two groups have a higher complete response during the 6- to 24-h period than the control group. ConclusionsRamosetron use led to a lower incidence, mild severity of nausea, and reduced use of rescue antiemetic drug after arthroscopic rotator cuff repair during the 6- to 24-h postoperative period than the control. Level of evidence : I, randomized controlled trials, treatment study
Testing mechanical chest compression devices for pre-hospital patient transport under resuscitation
Authors: Jörgens Maximilian , Körniger Jürgen , Kanz Karl-Georg , Birkholz Torsten , Hübner Heiko , Prückner Stephan , Zwissler Bernhard , Trentzsch Heiko 
Published: September 2020

Abstract
Background Mechanical chest compression (mCPR) offers advantages during transport under cardiopulmonary resuscitation. Little is known how devices perform en-route. Aim of the study was to measure performance of mCPR devices of different design during pre-hospital transport.Methods We tested animax mono (AM), autopulse (AP), corpuls cpr (CC) and LUCAS2 (L2). The route had 6 stages (transport on soft stretcher or gurney involving a stairwell, trips with turntable ladder, rescue basket and ambulance including loading/unloading). Stationary mCPR with the respective device served as control. A four-person team carried an intubated and bag-ventilated mannequin under mCPR to assess device-stability (displacement, pressure point correctness), compliance with 2015 ERC guideline criteria for high-quality chest compressions (frequency, proportion of recommended pressure depth and compression-ventilation ratio) and user satisfaction (by standardized questionnaire).Results All devices performed comparable to stationary use. Displacement rates ranged from 83% (AM) to 11% (L2). Two incorrect pressure points occurred over 15,962 compressions (0.013%). Guideline-compliant pressure depth was > 90% in all devices and compression-ventilation ratio was 40% (AM), 87% (CC, L2 respectively) and 93% (AP). Electrically powered devices showed constant frequencies while muscle-powered AM showed more variability (median 100/min, interquartile range 9). Although physical effort of AM use was comparable (median 4.0 vs. 4.5 on visual scale), participants preferred electrical devices.Conclusion All devices showed good to very good performance although device-stability, guideline compliance and user satisfaction varied by design. Our results underline the importance to check stability and connection to patient under transport.
Somatic Embryogenesis and Plant Regeneration via Solid-liquid Alternating Culture in Elite Genotypes of Upland Cotton
Authors: Liu Zhengjie , Wang Xinwang , Hua Jinping 
Published: September 2020

Abstract
Background: Cotton is one of the most genotype-dependent plants for regeneration, in order to expand cotton regeneration genotypes and establish efficient regeneration system platform, Jiwu 2031 (glandless cotton), ND 58 and CAU 102, were selected for studying the highly efficient somatic embryos formation and regeneration via solid-liquid alternating culture system.Results: In present research, the MSB medium (MS salts adding B5 vitamins) containing 0.571 µM indole-3-acetic acid (IAA), 0.465 µM kinetin (KT) and 0.904 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) was effective for callus initiation of fourteen Upland cotton with ‘Corker 201’ as the control. ‘Jiwu 2031’, ‘ND 58’ and ‘CAU 102’ could form somatic embryos and regenerate fertile plants in a solid MSB medium containing 10.31 mM NH4NO3, and supplemented with 0.027 mM glycin, 2.460 µM indole-3-acetic acid (IBA), 0.930 µM KT, 3.784 mM asparagine and 6.843 mM glutamine. Under the solid-liquid alternating culture system, the non-embryonic callus was induced to form embryonic callus and the identical status somatic embryos were obtained in 42 days, and the period for plants regeneration was shortened to 90~120 days, with the higher proportion of normal plant regeneration.Conclusions: The solid-liquid alternating culture method could increase the rate of embryogenesis and shorten the period of plants regeneration of Upland cotton. This work provides the evidence that the glandless cotton is beneficial for somatic embryogenesis (SE) and plant regeneration.
The prevalence of Trichinella spiralis in farmed minks (Neovison vison) associated with exposure to wild rats (Rattus norvegicus) in Shandong province, China
Authors: Zhang Nian-Zhang , Li Wen-Hui , Jin Qi-Wang , Yu Hai-Jie , Niu Dong-Yu , Chen Wei-Gang , Liu Ying-Ju , Qin Hong-Tao , Li Li , Yan Hong-Bin , Jia Wan-Zhong , Fu Bao-Quan 
Published: September 2020

Abstract
Background Both of American mink (Neovison vison) and wild rat (Rattus norvegicus) is considered reservoir hosts carrying many endoparasites. Trichinella is a successful parasitic nematode including nine species and three genotypes with a worldwide distribution. However, little is known about the prevalence of Trichinella infection in mink (Neovison vison) and rat (Rattus norvegicus) in China.Methods In total, 289 muscle samples of minks and 102 rat carcasses were collected between April 2017 and December 2019 in Weihai city of Shandong province, China. The food of minks including chicken skeleton and mashed marine products was also collected at 3 batches. All the samples were used to examine for the appearance of Trichinella by the pooled artificial HCl-pepsin digestion method. The isolates from minks and rats were identified as T. spiralis by multiplex PCR. Then, the phylogenetic tree was constructed based on the sequences of 5S rDNA inter-gene spacer regions.Results Muscle larvae were detected in 20 out of 289 minks (6.92%) and in 2 of 102 wild rats (1.96%), respectively. The intensity of Trichinella in mink samples was ranged from 0.025 to 0.815 lpg, while the larval burden in rats was 0.17 lpg. The isolates from minks and rats were identified as T. spiralis by multiplex PCR. Sequence analysis revealed a 100% identical alignment of the 5S rDNA inter-gene spacer regions from the two isolates. The phylogenetic tree confirmed the two isolates from minks and rats belonging to T. spiralis based on analysis of the 5S rDNA inter-gene spacer sequence.Conclusions The present study represents the first report of T. spiralis infection in American mink (Neovison vison) and wild rat (Rattus norvegicus) from Shandong province, China. The farmed minks would be vulnerable to Trichinella infection through exposure to the wild rats. The prevalence of T. spiralis in wild rats may raise a public health concern for the potential zoonotic risk for the domestic animals.