bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Case of Vasitis Nodosa with Biphenotypic Differentiation
Authors: Raju Beth Susan , Heinlen Jonathan , Luo Wenyi 
Published: July 2020

Abstract
Background

Vasitis nodosa is a nodular lesion within the vas deferens that is characterized by a benign glandular proliferation of the vas deferens epithelium mixed with sperm and chronic inflammation. It is considered a reactive condition caused by increased intraluminal pressure. Problems in diagnosis can arise when atypical morphologic features are present or when vasitis nodosa is colonized by metastatic tumors.
Case presentation:

A 25-year old African American male status post vasectomy was diagnosed with obstructive azoospermia and elected to undergo a vasovasostomy. The histology of the resected specimen was consistent with vasitis nodosa, but the proliferative epithelial cells demonstrated the phenotypes of both vas deferens epithelium and mesothelium by immunohistochemistry.
Conclusion

This is the first reported case of vasitis nodosa expressing mesothelial markers, and it may therefore mimic mesothelial tumors such as mesothelioma and adenomatoid tumor.
Establishment of a murine, lipopolysaccharide-induced sepsis model for testing anaerobic exercise thresholds and early mobilization
Authors: Matsuishi Yujiro , Shimojo Nobutake , Hoshino Haruhiko , Enomoto Yuki , Mathis Bryan J. , Oh Sechang , Kawano Satoru , Myoenzono Kanae , Maeda Seiji , Shoda Junichi , Inoue Shigeaki , Inoue Yoshiaki 
Published: July 2020

Abstract
Background: Several methods have been proposed to prevent post intensive care syndrome (PICS) after sepsis, including early mobilization, but controversy remains in mechanisms and outcomes. Translational studies are required to fully reveal the optimal exercise intensity during acute phase sepsis. The aim of this study is to establish a septic mouse model and and classify exercise intensi ties during the acute phase of sepsis. Methods: Adult, male C57/B6 mice received lipopolysaccharide (LPS) injections (20mg/kg). We recorded survival rates and metabolic changes, such as resting energy expenditure (REE) compared with controls up to 72h after LPS administration. We also observed vital signs (rectal temperature and weight change) and inflammation (TNF-αand IL-6). We detected exercise intensity via metabolic monitoring treadmill, analyzed the anaerobic threshold (AT) by V-Slope Method and classified the intensity levels according to American College of Sports Medicine (ACSM) guidelines.Result: Around 30% of the mice survived 72h after LPS induction with REE and rectal temperatures lowest at around 22-24h later (REE at 22h: 2.26±0.34 kcal/day p<0.05 vs. baseline; Rectal temperature at 24h: 29.17±0.48℃, p<0.05 vs. baseline). Gradual recovery was seen out to 72h. Blood concentrations of TNF-α were highest at the 12h timepoint (41.23±24.39 pg/ml, p<0.05 vs. baseline) and IL-6 was highest at 24h after LPS induction (1476.5±274.7 pg/ml, p<0.05 vs. baseline). Using the V‐Slope method, we observed the AT at each timepoint (6h:14m/min,12h: 9m/min, 24h:4m/min,36h:10m/min, 48h:16m/min,60h:20m/min,72h:22m/min) and classified these into very low intensity exercise training (VLIT), low intensity exercise training (LIT), or moderate intensity exercise training (MIT) at each timepoint. Conclusion: We classified exercise intensities out to 72h after sepsis recovery using a mouse model. These data may serve as a bridge to clinical studies to fill gaps in best practice for sepsis rehabilitation.
RNA-binding protein RBM3 negatively regulates innate lymphoid cells (ILCs) and lung inflammation
Authors: Badrani Jana , Amadeo Michael , Cavagnero Kellen , Naji Luay , Lund Sean , Leng Anthea , Lecasa Lee , Strohm Allyssa , Kim Hyojoung , Baum Rachel , Khorram Naseem , Mondal Monalisa , Seumois Greggory , Pilotte Julie , Vanderklish Peter , DOHERTY Taylor A 
Published: July 2020

Abstract
Innate lymphoid cells (ILCs) promote lung inflammation through cytokine production in diseases such as asthma. RNA-binding proteins (RBPs) are critical post-transcriptional regulators of cellular function, including inflammatory responses, though the role of RBPs in innate lymphoid cells is unknown. Here, we demonstrate that RNA-binding motif 3 (RBM3) is one of the most highly expressed RBPs in Thy1.2 + lung ILCs after fungal allergen challenge and is further induced by epithelial cytokines TSLP and IL-33 in both human and mouse ILCs. Single (rbm3-/-) and double (rbm3-/-rag2-/-) knockout mice exposed via the airway to the asthma-associated fungal allergen Alternaria alternata displayed increases in eosinophilic lung inflammation and ILC activation compared to control mice. In addition to increased Th2 cytokine production, rbm3-/- ILCs produced elevated IL-17A. The negative regulation by RBM3 in ILC responses was direct as purified rbm3-/- ILCs were hyperinflammatory in vitro and in vivo after stimulation with IL-33. Transcriptomic analysis by RNA-sequencing of rbm3-/- lung ILCs showed increased type 2 and 17 cytokines as well as global expression differences in critical cytokines, receptors, transcription factors, and survival transcripts compared with WT ILCs. Intriguingly, these transcript changes did not correlate with the presence of AU-rich elements (AREs), which RBM3 is known to bind. Thus, regulation of ILC responses by RNA-binding proteins offers novel mechanistic insight into lung ILC biology and ILC-driven inflammatory diseases.
High-capacity, transparent and robust blind audio watermarking scheme based DWT and TLBO algorithm
Authors: Joudaki Ali , Abdeyazdan Marjan , Mosleh Mohammad 
Published: July 2020

Abstract
Digital watermarking is one of the best solutions again the copyright infringement, duplicates, verifies data and illegal distribution of digital media. Recently, the protection of digital audio signals is one of the attracting and interesting topics for scientific and researchers. In this paper we propose a blind audio watermarking mechanism in which it has high capacity, transparency and resistance simultaneously based on digital wavelet transform (DWT) algorithm. The key principle of this work is that in the DWT procedure, using two filters; break down the original audio signal into several sub-bands and transform them on a specific frequency range. It should be noted that the 8 bits of watermarked signal is selected and transform to the original signal. In order to increase the watermarking resistance, framing the high frequency coefficients of the third level of the wavelet and calculate the frames average and place them in the frame memory prime. Moreover, TLBO algorithm used to determination of embedding and extraction coefficients in order to increase the SNR ratio in the embedding process and decrease the bit error rate (BER) in the extraction process. This method increases the embedding payload capacity while the audio SNR and extracted image BER have good qualify. Moreover, experimental results shown that this method has 13kbs hiding rate,ascendency imperceptibility, good payload capacity and intense robustness when resisting against various attacks such as MP3 compression, re-quantization, low-pass filtering, amplitude scaling, re-sampling, echo addition and noise corruption.
Diversity of KIR genes and their HLA-C ligands in Ugandan populations with historically varied malaria transmission intensity
Authors: Tukwasibwe Stephen , Traherne James A. , Chazara Olympe , Jayaraman Jyothi , Trowsdale John , Moffett Ashley , Jiang Wei , Nankabirwa Joaniter I. , Rek John , Arinaitwe Emmanuel , Nsobya Samuel L. , Atuheirwe Maxine , Mubiru Frank , Anguzu Godwin , Jagannathan Prasanna , Cose Stephen , Kamya Moses R. , Dorsey Grant , Rosenthal Philip J. , Colucci Francesco , Nakimuli Annettee 
Published: July 2020

Abstract
Background: Malaria is one of the most serious infectious diseases in the world. The malaria burden is greatly affected by human immunity, and immune responses vary between populations. Genetic diversity in KIR and HLA-C genes, which are important in immunity to infectious diseases, is likely to play a role in this heterogeneity. Several studies have shown that KIR and HLA-C genes influence the immune response to viral infections, but few studies have examined the role of KIR and HLA-C in malaria infection, and these have used low-resolution genotyping. Our aim was to determine whether genetic variation in KIR and their HLA-C ligands differ in Ugandan populations with historically varied malaria transmission intensity using more comprehensive genotyping approaches. Methods: We used high throughput multiplex quantitative real-time PCR method to genotype KIR genetic variants and copy number variation and developed a high-throughput real-time PCR method to genotype HLA-C1 and C2 allotypes for 1,344 participants, aged 6 months to 10 years, enrolled from Ugandan populations with historically high (Tororo District), medium (Jinja District) and low (Kanungu District) malaria transmission intensity. Results: The prevalence of KIR3DS1, KIR2DL5, KIR2DS5 and KIR2DS1 genes was significantly lower in populations from Kanungu compared to Tororo (7.6% vs. 13.2%: p=0.006, 57.2% vs. 66.4%: p=0.005, 33.2% vs. 46.6%: p<0.001 and 19.7% vs. 26.7%: p=0.014 respectively) or Jinja (7.6% vs.18.1%: p<0.001, 57.2% vs. 63.8%: p=0.048, 33.2% vs. 43.5%: p=0.002 and 19.7% vs. 30.4%: p<0.001 respectively). The prevalence of homozygous HLA-C2 was significantly higher in populations from Kanungu (31.6%) compared to Jinja (21.4%), p=0.043, with no significant difference between Kanungu and Tororo (26.7%), p=0.296. Conclusions: The KIR3DS1 , KIR2DL5, KIR2DS5 and KIR2DS1 genes are potentially beneficial in malaria as these genes have been positively selected for in places with historically high malaria transmission intensity. The high-throughput multiplex real-time HLA-C genotyping PCR method we have developed will be useful in disease association studies involving large cohorts.
Geomagnetic secular variation forecast using the NASA GEMS ensemble Kalman fllter: A candidate model for IGRF 2020
Authors: Tangborn Andrew , Kuang Weijia , Sabaka Terence , Yi Ce 
Published: July 2020

Abstract
We have produced a 5 year mean secular variation (SV) of the geomagnetic field for the period 2020-2025. We use the NASA Geomagnetic Ensemble Modeling System (GEMS), which consists of the NASA Goddard geodynamo model and ensemble Kalman filter (EnKF) with 512 ensemble members. Geomagnetic field models are used as observations for the assimilation, including gufm1 (1590-1960), CM4 (1961-2000) and CM6 (2001-2019). The forecast involves a bias correction scheme that assumes that the model bias changes on timescales much longer than the forecast period, so that they can be removed by successive forecasts. The algorithm was validated on the time period 2010-2015 by comparing with the 2015 IGRF before being applied to the 2020-2025 time period. This forecast has been submitted as a candidate model for IGRF 2025.
Thyroid metastases from breast carcinoma: case report and review of literature
Authors: Wang Yichao , Zhou Shengliang , Yu Boyang , Zhou Ping , Li Zhihui , Zhu Jingqiang , Wei Tao 
Published: July 2020

Abstract
Rationale: The thyroid is a rare site for distant metastases from breast carcinoma. The incidence of thyroid metastases in fine needle aspiration biopsy (FNAB) was less than 0.2%.Patient concerns: We report a case of 54-year-old woman with a history of breast carcinoma presented with diffuse scattered microcalcifications in thyroid and enlarged bilateral cervical lymph nodes detected on ultrasound (US). Physical examination of the patient revealed stiff and enlarged thyroid lobes.Diagnoses: FNAB and immunohistochemistry （IHC）of the thyroid lesion confirmed the thyroid metastases from breast cancer.Interventions and outcomes: Due to the comorbidities of breast carcinoma metastases to the right axillary, cervical lymph nodes and left chest wall, the patient received chemotherapy. After a follow-up of 10 months, the patient was alive without any new distant metastases.Lessons: Our case highlights that thyroid metastases should be considered in a patient combined with thyroid lesions and a history of breast carcinoma. IHC played an important role in differentiating thyroid metastases from primary thyroid cancer.
Left Ventricular Free Wall Rupture in A Middle-Aged Woman
Authors: Gonçalves Fernando Fonseca , Monteiro João Pedro , Neves Fátima São , Moreira José Ilídio 
Published: July 2020

Abstract
Background: Ventricular free wall rupture is an uncommon but potentially fatal mechanical complication following a myocardial infarction.Case presentation: We report a case of a 51 year-old female admitted in our emergency department in cardiogenic shock, in which echocardiography was fundamental in the diagnosis of a left ventricle free wall rupture with tamponade. In this case, removal of pericardial clots with subsequent hemodynamic improvement and the presence of a strong adhesion between the pericardial layers at the rupture site were sufficient for the short-term surgical treatment. The patient remained stable and was discharged fifteen days later.Conclusions: Although being a rare presentation, this unconventional surgical approach might be useful in this kind of cases.
Clinical characteristics and risk factors for mortality in patients with coronavirus disease 2019 in intensive care unit: a single-center, retrospective, observational study in China
Authors: Sai Fangfang , Liu Xiaolei , Li Lanyu , Ye Yan , Zhu Changqing , Hang Ying , Huang Conghua , Tian Lei , Xu Xinhui , Huang Huan 
Published: July 2020

Abstract
Background: Coronavirus disease 2019 (COVID-19) is a potentially life-threatening contagious disease which has spread all over the world. Risk factors for the clinical outcomes of COVID-19 pneumonia in intensive care unit (ICU) have not yet been well determined. Methods: In this retrospective, single-centered, observational study, we consecutively included 47 patients with confirmed COVID-19 who were admitted to the ICU of Leishenshan Hospital in Wuhan, China, from February 24 to April 5, 2020. Clinical characteristics and outcomes were collected and compared between survivors and non-survivors. Multivariable logistic regression was used to explore the risk factors associated with death in patients of COVID-19.Results: The study cohort included 47 adult patients with a median age of 70.55±12.52 years, and 30 (63.8%) patients were men. Totally 15 (31.9%) patients died. Compared with survivors, non-survivors were more likely to develop septic shock (6 [40%] patients vs 3 [9.4%] patients ), disseminated intravascular coagulation (3 [21.4%] vs 0), and had higher score of APACHE II (25.07±8.03 vs 15.56±5.95), CURB-65 (3[2-4] vs 2[1-3]), Sequential Organ Failure Assessment (SOFA) (7[5-9] vs 3[1-6]), higher level of D-dimer (5.74 [2.32-18] vs 2.05 [1.09-4.00] ) and neutrophil count (9.4[7.68-14.54] vs 5.32[3.85-9.34] ). SOFA score (OR 1.47, 1.01–2.13; p=0.0042) and lymphocyte count (OR 0.02, 0.00–0.86; p=0.042) on admission were independently risk factors for mortality. Patients with higher lymphocyte count (>0.63×109/L) and lower SOFA score ≤4 on admission had a significantly well prognosis than those with lower lymphocyte count (≤0.63×109/L) and higher SOFA score >4 in overall survival.Conclusions: Higher SOFA score and lower lymphocyte count on admission were associated with poor prognosis of patients with COVID-19 in ICU. Lymphocyte count may serve as a promising prognostic biomarker.
A novel surgical approach and technique and short-term clinical efficacy for the treatment of proximal humerus fractures with the combined use of medial anatomical locking plate fixation and minimally invasive lateral locking plate fixation
Authors: Wang Fu , Wang Yan , Dong Jinye , He Yu , Li Lianxin , Liu Fanxiao , Dong Jinlei 
Published: July 2020

Abstract
Background and hypothesis: The typical anterolateral approach is widely used to treat proximal humerus fractures with lateral locking fixation. However, lateral fixation cannot completely avoid medial reduction loss and varus deformity especially in the cases of an unstable medial column. We present a novel medial surgical approach and technique together with a minimally invasive lateral locking plate to fix proximal humerus fractures with an unstable medial column.Materials and Methods: An anatomical study was performed and an anatomical medial locking proximal humerus plate was designed based on the measurement results. Between November 2014 and April 2017, eight cases of proximal humerus fractures with unstable medial columns underwent medial fixation. All surgeries were performed by a single surgeon. Patients were followed clinically and radiographically at 1, 3, 6 and 12 months postoperatively.Results: There was a safe region located at the medial part of the proximal humerus just beneath the articular surface. An anatomical medial locking proximal humerus plate could be placed in the medial column and did not affect the axillary nerve, blood supply of the humeral head or stability of the shoulder joint. Successful fracture healing was achieved in all 8 cases. The function and range of motion of the shoulder joint were satisfactory 24 months postoperatively, with an average Constant score (CS) of 82.8. No reduction loss (≥ 10 degrees in any direction), screw cutout, nonunion, or deep infection occurred.Conclusions: The combined application of medial anatomical locking plate fixation and minimally invasive lateral locking plate fixation is effective in maintaining operative reduction and preventing varus collapse and implant failure in proximal humerus fractures with an unstable medial column.Level of evidence: Retrospective Study
Diversity and Endogenous Knowledge of Aphrodisiac Plants in South and Central Benin
Authors: Batcho Ismaël Akossibe , Ewédjè Eben-Ezer Baba Kayodé , Yédomonhan Hounnankpon , Adomou Aristide Cossi 
Published: July 2020

Abstract
Background: The use of aphrodisiac plants is a very common and ancient practice in Africa. This study, conducted in central and southern Benin, aimed to document endogenous knowledge related to flora, valorization, promotion and conservation of plants species used for aphrodisiac purposes.Methods: Data were collected using ethnobotanical and market surveys from 134 people followed by observations.Results: A total of 138 aphrodisiac plants species were recorded, including 72 from Center Benin and 20 from South Benin while 46 species were common to both areas. These species belong to 124 genera from 59 families mostly represented by Fabaceae (14.49%), Euphorbiaceae (8.76%), and Poaceae (4.38%). The predominant biological type was Phanerophytes (57.25%). The chorological analysis identified a dominance of Sudano-Guinean (26.09%), Pantropical (24.64%) and Guinea-Congolian species (19.57%). Aphrodisiac plants were used for 220 recipes to treat eight affections from which the common was sexual weakness (88.80%). Roots (33.12%) and leaves (20.13%) were the most used parts. The maceration (44.76%) and oral route (88.11%) were the main galenic form and the main mode of administration, respectively.Conclusion: This flora represents a raw material for subsequent phytochemical characterization to identify new aphrodisiac properties, leading to produce Improved Traditional Medicines against sexual disorders.
Synthesis of Oxadiazole-2-Oxide Derivatives as Potential Drug Candidates for Schistosomiasis Targeted on SjTGR
Authors: sun dequn , Li Gongming , Guo Qingqing , Feng Chao , Chen Huan , Zhao Wenjiao , Li Shu , Hong Yang 
Published: July 2020

Abstract
Background: Schistosomiasis is a chronic parasitic disease that affects the health of millions people worldwide. Developing new drugs to treat schistosomiasis is crucial because of the increasing drug resistant to praziquantel (PZQ), the main drug for schistosomiasis. Oxadiazole-2-oxides have been identified as a potential anti-schistosomiasis reagent targeted to thioredoxin glutathione reductase (TGR).Methods: In this work, one of the oxadiazole-2-oxides derivatives furoxan was used as the lead compound to exploit series of novel furoxan derivatives for studying inhibitory activity against both recombinant Schistosoma japonicumi TGR containing selenium (rSjTGR-Sec) and soluble worm antigen protein (SWAP) containing wild type Schistosoma japonicumi TGR (wtSjTGR) in order to develop new leading compound for schistosomasis. Thirty nine novel derivatives were prepared to test their activity to both rSjTGR-Sec and SWAP containing wtSjTGR. The docking method was used to detect the bind sites between active molecule and SjTGR; The structure-activity relationship (SAR) of these novel furoxan derivatives was preliminarily analyzed.Results: It was founded that several new derivatives such as compounds 6a-6d, 9ab, 9bd, 9be have better activity to rSjTGR-Sec or SWAP containing wtSjTGR than furoxan. Interestingly, all intermediates bearing hydroxy (6a-6d) showed excellent inhibitory activity to both rSjTGR-Sec and SWAP containing wtSjTGR especially, compound 6d with trifluoromethyl on pyridine ring was found to have much higher inhibition to both rSjTGR-Sec (half-maximal inhibitory concentration, IC50,7.5nM) and SWAP containing wtSjTGR (IC50 55.8nM) than furoxan (4µM to SWAP containing wtSjTGR and 5.87µM to rSjTGR-Sec). Additionally, the docking method revealed the matching sites between 6d and Schistosoma japonicumi TGR (SjTGR), which theoretically lends support to inhibitory activity of 6d. Conclusion: The data obtained herein showed preliminarily that 6d with trifluoromethyl on pyridine ring could be a valuable leading compound for further study.
Bioinformatics-based Prediction of the mechanism and targets for BushenjieduDecoction in preventing relapse of acute leukemia
Authors: Sun Weilong , Yang Fujun , Shi Weipeng , Tao Xia , Xi Zhiwei , Liu Yihao , Xu Xinxin , Yang Xiangdong 
Published: July 2020

Abstract
Background: Leukemia is a lethal myeloproliferative disorder, its’ relapse following chemotherapy is the major concern in clinical practice. For a long time, we found that traditional Chinese medicines such as Bushenjiedudecoction (BSJD) have significant effects on delaying relapse. However, the underlying mechanisms are not clear, which limits the clinical application of BSJD decoction. Methods: Therefore, we tried to make some explorations in this study. We isolated mesenchymal stem cells (MSC) after treated them with BSJD for proteomic analysis. And then 109 targets were screened out through analysis of the shared proteins of that affected by BSJD and those related to leukemia. Subsequently, the data were analyzed by GO functions, KEGG pathways, PPI network and topological analysis, and then some nodes were selected for animal experiment. Results: As a result, we demonstrated the effective targets of BSJD on MSC through bioinformatics analysis and explored the potential mechanism of BSJD from its influence on niches.These targets contains Hspb1、Dnmt1、Mmp2、Thbs1、Crebbp、Hmgb1、Acta2、Cdkn1b、Atg7、Tsc2 and Icam1. Afterwards, we confirmed BSJD reduced the gene expression of ICAM-1 through cultured MSC in vitro.Conclusions: We screened the potential targets of BSJD on MSC through proteomics and bioinformatics analysis, and selected some genes for experimental verification. These studies demonstrated the effect of BSJD on MSC. We hope that this research method could provide a new way of systematically studying the effects of traditional Chinese medicine on diseases.