bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of Kluyveromyces marxianus as a novel host for large-scale production of porcine parvovirus virus-like particles
Authors: Yang Deqiang , Chen Lei , Duan Jinkun , Yu Yao , Zhou Jungang , Lu Hong 
Published: September 2020

Abstract
Background: Porcine Parvovirus (PPV) is a Parvovirinae virus that can cause embryonic and fetal loss and death and mummification in affected fetal pigs. Unlike conventional vaccines, virus-like particles (VLPs) inherit the natural structure of their authentic virions and highly immunostimulatory that can induce strong humoral immune and T cell responses with no risk of pathogenicity due to incomplete attenuation or inactivation. Production PPV VLPs is still a challenge using the traditional expression platforms due to the low yield and culture cost. Kluyveromyces marxianus is a safe and fast-growing eukaryote that can yield high biomass in low-cost cultures. In this study, we investigated the production and downstream processes of PPV VLPs in K. marxianus, and the potential for effective stand-alone vaccines.Results: After optimization according to the codon bias of K.marxianus, the VP2 protein from Kresse strain was highly expressed. In 5L fermentator, the yield of PPV VLPs reached 2.5 g/L, qualified by HPLC, using a defined mineral medium after 48h fermentation. Two strategies, cation exchange chromatography coupled with Sephacryl® S-500 HR chromatography for the supernatants of pH adjusted cell lysates and anion exchange chromatography followed by cross-flow diafiltration for the precipitate respectively, were established to purify the intracellular PPV VLPs. The purity PPV VLPs reached about 95%, and total recovery was more than 60%. Sera from the PPV VLPs immunized mice showed high titers of IgG antibody and hemagglutination inhibitions on both swine and guinea pig erythrocytes. Spleen lymphocyte proliferation and cytokines detection suggested the PPV VLPs produced by K.marxianus can provoke the cellular immune and humoral immunity responses.Conclusion: This is the highest production of recombinant PPV VLPs achieved to date. The superiorities, safe, high production, short lead time, and low cost, make K. marxianus a greatly competitive platform in bioproduction of PPV VLPs vaccine.
Opportunistic Routing Based on Partial CSI in MIMO Random Ad-hoc Networks
Authors: Richter Yiftach , Bergel Itsik 
Published: September 2020

Abstract
In this paper we consider opportunistic routing in multiple-input-multiple-output (MIMO) random wireless ad-hoc networks (WANETs). Our analysis uses a proper model of the physical layer together with an abstraction of the higher communication layers. We assume that the nodes are distributed according to a Poisson-Point-Process and consider a routing scheme that opportunistically selects the next relay and the number of spatially multiplexed data streams. The routing decisions are based on geographic locations, the channel gains of the neighbor nodes and the statistical characterization of all other nodes. Unlike the single antenna case, the optimal routing scheme cannot be explicitly expressed. Hence, we propose a smart-routing scheme for MIMO that adapts the number of data streams per user to the channel conditions. Numerical results demonstrate that this scheme outperforms all previously published schemes for this scenario. The findings highlight the importance of channel state information for efficient routing, and the need for an adaptive selection of the number of data streams at each transmitter.
Anthraquinone Laxative-Altered Gut Microbiota Induces Colonic Mucosal Barrier Dysfunction for Colorectal Cancer Progression
Authors: Wei Zhonghong , Wu Jiawei , Yang Yu , Shen Peiliang , Cheng Peng , Tao Li , Shan Yunlong , Sun Zhiguang , Lu Yin 
Published: September 2020

Abstract
Background: The safety of anthraquinone laxatives has been controversial. Previously, a few scattered studies have revealed that anthraquinones can cause the damage to the structure of colonic epithelial tissue, and long-term intake of anthraquinone laxatives increase the colorectal cancer risks. In this study, we focused on sennoside A, a primary purgative component in anthraquinone laxatives, and investigated the effects of sennoside A on intestinal inflammation, host metabolism, and whether it can exacerbate the colitis-associated colon cancer in the AOM/DSS mouse model. Results: Initially, sennoside A disrupted the mucus layer and mechanical barrier of the colon. The dynamic effects of sennoside A on the community structure of gut microbiota were futher anlyazed. We found sennoside A significantly promoted the dominant growth of Akkermansia muciniphila after continuous treatment for 56 days, which was independent of the prototype of sennoside A and its metabolites, including the main host metabolite rhein and bacteria metabolites. Interestingly, sennoside A suppressed the growth of butyrate-producing bacteria, thereby decreasing the butyrate levels. Besides, sennoside A directly inhibited the growth of Clostridium tyrobutyrate and Clostridium butyricum, which was not caused by the prototype of sennoside A , but the direct bacteriostatic effect produced from its metaboliterhein. Lastly, supplement with butyrate prevented sennoside A-induced gut dysbiosis and mucus barrier impairment. Conclusions: our work reveals that sennoside A can impair the integrity of the intestinal mucosal barrier, which is closely related to the disruption of the structural balance between mucus-degrading bacteria and exogenous fibro-degrading bacteria, inducing long term low-grade inflammation, metabolic disorders associated with tumorigenesis, and consequently promoting the progression of colonic carcinogenesis in the AOM/DSS mouse model.
Upgraded adenine base editor (uABE) with minimized RNA off-targeting activity
Authors: Li Jianan , Yu Wenxia , Huang Shisheng , Wu Susu , Li Liping , Zhou Jiankui , Cao Yu , Huang Xingxu , Qiao Yunbo 
Published: September 2020

Abstract
Both adenine base editors (ABEs) and cytosine base editors (CBEs) have been recently revealed to induce transcriptome-wide RNA off-target editing in a guide RNA-independent manner. As the optimized ABE, ABEmax, induces highly efficient A-to-I (inosine) editing within an E.coli tRNA-like structure, we construct a reporter system containing E.coli Hokb gene with a tRNA-like motif for robust detection of RNA editing activities. Then, we design mutations to disrupt the interaction between TadA and tRNAs in structure-guided principles, and find that Arginine 153 (R153) within TadA is essential for recognizing core tRNA-like structures. Two ABEmax or mini ABEmax variants (TadA* fused with Cas9 (D10A)) with deletion of R153 within TadA and/or TadA* (named as del153/del153* and mini del153) are successfully engineered, showing minimized RNA editing, but comparable DNA on-targeting activities. Moreover, del153 in recently reported ABE8e or ABE8s can also largely reduce their RNA off-targeting activities. Taken together, we develop a strategy to generate upgraded ABEs (uABEs) with minimized RNA off-target activities.
Takotsubo Syndrome Induced by Brachytherapy in a Patient with Endocervical Adenocarcinoma.
Authors: Vieira Aline Cristini , Ribeiro Mauricio Fernando Silva Almeida , Lima Julianne , Filho Jacob Sessim , Carvalho Heloisa de Andrade , Mano Max Senna 
Published: September 2020

Abstract
Background: Takotsubo syndrome (TTS), also known as stress cardiomyopathy, apical ballooning syndrome and broken heart syndrome, is characterized by acute-onset chest pain, electrocardiographic (ECG) abnormalities and reversible left ventricular (LV) disfunction in the absence of a culprit obstructive lesion in the coronary arteries; therefore, myocardial infarction is the most important differential diagnosis. Usually induced by emotional/physical stress, its treatment consists in hemodynamic support until complete and spontaneous recovery occurs, which is generally achieved within a few days to weeks. Cervical malignancies are an important public health issue in low/middle-income countries and, in the setting of locally advanced disease, concurrent chemoradiation followed by brachytherapy is considered the standard treatment, harboring curative potential. Case report: We report a case of a 38-year-old woman who underwent concurrent chemoradiotherapy and developed cardiopulmonary arrest in ventricular fibrillation during a brachytherapy session. Complementary tests disclosed altered EKG and cardiac biomarkers, no evidence of coronary artery obstruction, as well as LV disfunction consistent with TTS on echocardiogram and cardiac MRI. After few days of supportive therapy, complete recovery of heart function was observed. Conclusion: considering that community clinicians might be unfamiliar with TTS, this condition is likely to be underdiagnosed. Especially for cancer patients, who usually experience intense emotional/physical stress intrinsically associated with their diagnosis and aggressive treatments, considering TTS as a differential diagnosis is warranted. Intracavitary brachytherapy procedure may represent a trigger for TTS.
Analysis of Vaginal and Endometrial Microbiota Communities in Infertile Women With a History of Repeated Implantation Failure
Authors: Ichiyama Takuhiko , Kuroda Keiji , Nagai Yoko , Urushiyama Daichi , Ono Motoharu , Yamaguchi Takashi , Nagayoshi Motoi , Sakuraba Yoshiyuki , Yamasaki Fumio , Hata Kenichiro , Miyamoto Shingo , Itakura Atsuo , Takeda Satoru , Tanaka Atsushi 
Published: September 2020

Abstract
Background: Repeated implantation failure (RIF) is estimated to occur in 15%–20% of infertile women undergoing in vitro fertilization-embryo transfer (IVF-ET). Molecular identification recently confirmed that the uterine microbiota may have implications for reproductive and obstetrical outcomes. One hundred forty-five women who had been diagnosed with RIF were enrolled in the study. Twenty-one healthy women were also enrolled as controls. We investigated their vaginal and endometrial microbiotas using 16S rRNA gene sequencing and compared the microbiota profiles in the patients with RIF and controls.Results: The endometrial microbiotas had higher α-diversities than did the vaginal microbiotas (p<0.001 in both patients with RIF and healthy women). The microbiota profiles showed that vaginal and endometrial samples in patients with RIF had significantly higher levels of 5 and 14 bacterial genera, respectively, than those in healthy women. These genera included Atopobium, Gardnerella, Prevotella and Megasphaera. Vaginal Lactobacillus rates in patients with RIF were significantly lower at 76.4 ± 38.9% compared with those of the controls at 91.8 ± 22.7% (p=0.018), but endometrial Lactobacillus rates did not significantly differ between the RIF patients and controls (56.2 ± 36.4% and 58.8 ± 37.0%, respectively, p=0.79) Conclusions: Impaired microbiota communities containing specific bacteria in both the endometrium and vagina were associated with implantation failure. The Lactobacillus rate in the vagina, but not the endometrium, may be a biomarker for RIF.Trial registration: UMIN Clinical Trials Registry, UMIN000031731, Registered 15 March 2018; https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000036121
Family Planning for Women With Severe Mental Illness in Rural Ethiopia: A Qualitative Study
Authors: Zerihun Tigist , Sorsdahl Katherine , Hanlon Charlotte 
Published: September 2020

Abstract
Background: Family planning is a crucial issue for all women of reproductive age, but in women with severe mental illness (SMI) there may be particular challenges and concerns. As primary care-based mental health services are expanding in low- and middle-income countries (LMICs), there is an opportunity to improve family planning services for women with SMI. However, research exploring unmet family planning needs of women with SMI in such settings is scarce. Therefore, the present study explored the family planning experiences, unmet needs and preferences of women with SMI who reside in a predominantly rural area of Ethiopia Methods: A qualitative study design was used. Women with SMI who were participating in the ongoing population-based cohort study in Butajira were selected purposively on the basis of responses to a quantitative survey of current family planning utilization. In-depth interviews were conducted with 16 women with SMI who were of reproductive age. Audio files were transcribed in Amharic, translated into English and analyzed using the Framework Approach using Open Code qualitative data analysis software. Results: Participants reported pervasive effects of SMI upon the intimate relationships and sexual life of women. Although women with SMI felt that family planning was important, they had limited knowledge of family planning generally and a lack of understanding of the specific family planning needs relevant to having SMI. None of the women with SMI in the present study had received any recommendations to use family planning services while accessing mental health care services. The participants identified ways in which primary care-based mental health services could better meet their family planning needs. Conclusion: This study has provided in-depth perspectives from women with SMI about the broader context of their family planning experience, needs, barriers and how integrated primary care services could better meet their needs. Empowerment of women with SMI to access information and services needs to be an important focus of future efforts to improve the reproductive experiences of this vulnerable group.
The Treatment of Macrolide-Resistant Mycoplasma Pneumoniae Pneumonia in Children
Authors: DAI Fang-fang , LIU Feng-qin , CHEN Xing , YANG Juan , WANG Ke , GUO Chun-yan 
Published: September 2020

Abstract
Objective: To evaluate the efficacy and safety of levofloxacin in children with macrolide-resistant Mycoplasma pneumoniae pneumonia. Methods: We retrospectively enrolled six confirmed cases of refractory Mycoplasma pneumoniae pneumonia (n=6) who were admitted in the pediatric respiratory ward of Shandong provincial hospital Affiliated to Shandong first Medical University between January 1st, 2020and February 29th, 2020. Levofloxacin was given to the patients through the intravenous or oral route as per the following dosages :< 5 years, 8-10 mg/kg q12hours; > 5 years, 8-10 mg/kg, qd for ten days. The clinical data were collected and analyzed. Results: The average age of the enrolled cases was six years and nine months (range, four years, and seven months to eleven years and seven months). All cases were found to be drug-resistant and were treated with azithromycin combined with antibacterial drugs. Levofloxacin was used in the patient’s refractory to macrolide antibiotics. The temperature of five cases returned to normal 1 to 2 days after treatment with levofloxacin, and the imaging of the four cases showed expected improvements. The gastrointestinal symptoms, neurological manifestations, joint symptoms, blood parameters, liver and kidney functions, and exercise conditions of the children were closely monitored. The follow-up time of the patients ranged from one week to five months. No drug-related adverse reactions were observed in patients during treatment or during follow up.Conclusions: The clinical symptoms and imaging significantly improved after treatment with levofloxacin, and no drug-related adverse reactions were observed. Levofloxacin proved to be an effective and safe drug in the treatment of children with macrolide-resistant mycoplasma pneumonia.
Maternal Satisfaction on Delivery Care Services and Associated Factors Among Mothers who Gave Birth in the University of Gondar Teaching and Referral Hospital, Northwest Ethiopia
Authors: Firdie Andualem , Maru Alemnew , Deriye Abdulahi , Assefa Amare , Bedassa Abdi , Bekele Anteneh , Alemayehu Amanuel , Abebe Zegeye , Gizaw Zemichael 
Published: September 2020

Abstract
Introduction: Maternal satisfaction on delivery services is an important indicator for assessment of the quality of care. Quality of delivery care is the degree to which maternal health services for individuals and populations increase the likelihood of timely and appropriate treatment for the purpose of achieving desired outcomes. The use of services and outcomes are the result not only of the provision of care but also of women’s experience of that care. Therefore, investigating women’s experience or satisfaction on delivery care is of paramount importance to enhance the services utilization. Methods: An institution based cross-sectional study design was used. Data were collected using structured and pretested questionnaire from 403 mothers who were recruited by simple random sampling technique. Multivariable binary logistic regression was used to identify variables associated with maternal satisfaction on delivery services on the basis of adjusted odds ratio (AOR) with 95% confidence interval (CI) and a p – value less than 0.05. Results: The current study found that 78.2%, 65.5%, 64.3%, 49.9% and 44.7% of the mothers who gave birth at the University of Gondar teaching and referral hospital were satisfied on physicians’ communication, health care services, physicians’ attitude, privacy and sanitation condition of the health institution respectively. The overall satisfaction of mothers on delivery services was found to be 65.5%. Maternal satisfaction was statistically associated with diploma and above education [AOR = 0.29, 95% CI = (0.13, 0.66)]; secondary and/or primary education [AOR = 0.42, 95% CI = (0.20, 0.90)]; Antenatal care (ANC) follow-up in the current pregnancy [AOR = 4.47, 95% CI = (1.77, 11.27)] and short waiting time [AOR =1.85, 95% CI = (1.19, 2.88)].Conclusion: This study revealed that the overall satisfaction of mothers on delivery service was found to be suboptimal. Physicians’ communication, health care services and physicians’ attitude were areas where the highest proportion of mothers satisfied. On the other hand, the highest proportion of mothers was dissatisfied on sanitation condition. Educational status, ANC follow-up and waiting time were found to be statistically associated with maternal satisfaction on delivery services.
Circular RNA circDLC1 Inhibits MMP1-Mediated Liver Cancer Progression via Interaction with HuR
Authors: Liu Hailing , Lan Tian , Li Hui , Xu Lin , Chen Xing , Liao Haotian , Chen Xiangzheng , Du Jinpeng , Cai Yunshi , Wang Jinju , Li Xuefeng , Huang Jiwei , Yuan Kefei , Zeng Yong 
Published: September 2020

Abstract
Background: N6-methyladenosine (m6A) modification has been demonstrated to be closely related to cancer progression. KIAA1429, a key component of the m6A methyltransferase complex, has recently been reported to promote hepatocellular carcinoma (HCC) progression by regulating the m6A methylation. However, the involvement of circular RNAs (circRNAs) in KIAA1429-mediated HCC progression is still unknown.Methods: RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation sequencing (m6A-seq) were utilized to identify KIAA1429-regulated circRNAs. The effects of circDLC1 on proliferation and metastasis of hepatoma cells were examined in vitro and in vivo. RT-qPCR was used to measure the expression of circDLC1 in HCC tissues and hepatoma cells. RNA FISH, RIP assays and biotin-labeled RNA pull-down were used to investigate the downstream effector of circDLC1. The downstream targets of circDLC1 were identified using RNA-seq.Results: Our data demonstrated that circDLC1 was downregulated in HCC tissues and closely relevant to favorable prognosis. Overexpression of circDLC1 inhibited the proliferation and motility of hepatoma cells in vitro and in vivo, while silencing of circDLC1 played the opposite role. Mechanistic investigations revealed that circDLC1 could bind to RNA-binding protein HuR, which subsequently reduced the interaction between HuR and MMP1 mRNAs, thus inhibited the expression of MMP1, finally contributed to inhibition of HCC progression.Conclusion: Our work suggests that circDLC1, a downstream target of KIAA1429, is a promising prognostic marker for HCC patients, and the circDLC1-HuR-MMP1 axis may serve as a potential therapeutic target for HCC treatment.
Community Detection using Unsupervised machine learning technique on COVID -19 dataset
Authors: Chaudhary Laxmi , Singh Buddha 
Published: September 2020

Abstract
COVID-19 has been considered to be the most destructive pandemic ever happened in the history of mankind. The worldwide research community has put a tenacious effort to carry out research on the COVID-19 to analyse its impact on economic, medical and sociolgoical fields. They are trying to solve many crucial issues related to this disease and derive strategies to deal with this global pandemic. In this paper, we have analysed the trend, countries affected regionally and the variation of cases at the country level on COVID-19 dataset. We have used the Principal component analysis on the COVID-19 dataset variables to reduce the dimentionality and find the most significant variables. Further, we have unvieled the hidden community structure of countries by applying the unsupervised clustering approach, K-means. The resulted communities can be an advantage to researchers, politicians, sociologists, different policy makers and managers of health sector.
Similarity Comparisons of SARS-CoV-2 Samples between Wuhan and G20
Authors: Wang Zhenhui , Qiao Mu , Zheng Jeffrey 
Published: September 2020

Abstract
To analyze SARS CoV-2 genomes, this paper uses a viral genome collected in Wuhan as a basic sequence to select a set of genomes from G20 countries/regions for comparison. Two methods are applied in visualization followed in the A1 and C1 modules of the MAS to provide 1D similarity projection and information entropy approaches. For a pair of two genomes segmented as M segments to calculate base differences corresponding to each segment, a measuring vector can be generated to be projected as a visual map. Refined information will be provided to calculate the information entropy corresponding to base differences. This provides quantitative measures to organize these genomes arranged into a linear order. The first method uses a line chart representation to illustrate relevant differences among genomes 1 by 1 between Wuhan and other regions. The second method uses a histogram representation to observe the information entropy projection results. Significant similarities are observed among the differences with approximately equal entropies with higher similarity. Based on the comparison of gene sequence diagrams between Wuhan and various regions, a difference analysis was carried out.
Assessing the effects of exposure to a SARS-CoV-2 re-positive patient in healthcare personnel
Authors: Ogawa Yoshihiko , Nishida Koji , Gohma Iwao , Kasahara Kei , Yano Hisakazu 
Published: September 2020

Abstract
Objective: To evaluate whether patients with COVID-19 who have tested re-positive with the PCR test for the SARS-CoV-2 virus are infectious is a challenge in the current circumstances. A follow-up survey was conducted with healthcare personnel (HCP) who were exposed to a patient whose PCR test results for SARS-CoV-2 were re-positive 18 days after the initial confirmation of negative PCR results.Results: We studied a total of 15 HCP who had contact exposures (15/15) and aerosol exposures (7/15). None of them tested positive for IgG against SARS-CoV-2 on blood examination. None of them had any symptoms during 10 days of active isolation. All PCR tests conducted using the nasopharyngeal swabs collected from the HCP on day 10 were negative. No apparent infection was found in any of the HCP who had contact exposure with and/or aerosol exposure from the patient whose PCR test results for SARS-CoV-2 were re-positive 18 days after the initial confirmation of negative results of PCR tests for SARS-CoV-2.Trial Registration: No. 170, approved June 10th, 2020 by the ethics committee of Sakai City Medical Center.