bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glutamine metabolism model for predicting prognosis and immune infiltration in laryngeal squamous cell carcinoma
Authors: He Ming , Zhao Wenjing , Sun Feng , Shen Nimei , Han Liang 
Published: December 2024

Abstract

Background
Laryngeal cancer, a common malignant tumor of the head and neck, is increasingly prevalent and poses significant challenges to patient health and quality of life. The primary pathological type was squamous cell carcinoma. Previous studies have shown that genes involved in glutamine metabolism are crucial for throat cancer development; however, their prognostic significance in laryngeal cancer remains unexplored.
Methods
Data from laryngeal cancer patients were obtained from The Cancer Genome Atlas (TCGA), and glutamine (Gln) metabolism-related genes were sourced from the GeneCards database. Univariate Cox regression analysis, hub gene screening, and LASSO Cox regression identified five key genes linked to glutamine metabolism and LSCC prognosis. A risk score was calculated on the basis of median values to categorize patients into high-risk and low-risk groups. Clinical features, immune cell infiltration, and immunotherapy effects were systematically analyzed.
Results
The model was closely correlated with patient prognosis. The risk score model, which is based on five characteristic genes, demonstrated excellent predictive performance, as validated by receiver operating characteristic (ROC) curves and Kaplan‒Meier survival analysis. Significant differences in immune cell infiltration, gene expression, and survival status were observed among the different risk groups.
Conclusion
This prognostic model can effectively predict outcomes and immune infiltration in laryngeal squamous cell carcinoma (LSCC) patients.
Integrating HCY Metabolic Enzyme Gene Polymorphisms and Expression Data Reveals Key Genes in Ovarian Cancer
Authors: Zhang Yu , Liu Yuan , Gao Shan , Guo Yaqiong , Wei XueLing , Xu He 
Published: December 2024

Abstract

This study investigates the role of homocysteine (HCY) metabolic enzyme variants in ovarian cancer. HCY is a sulfur-containing non-protein amino acid and an important metabolic intermediate in the human body, with elevated HCY levels being linked to cancer by many researchers. We analyzed SNPs of six HCY metabolic enzymes using eQTL data to determine their effects on gene expression. By matching ovarian cancer gene expression data from the TCGA database and performing differential analysis using the GEO dataset GSE12470, we identified nine genes (MTHFR, PLOD1, U2AF, EIF2S2, EDEM2, MTR, WDR4, CHMP4B, AGTRAP) that were significantly differentially expressed in ovarian cancer patients. These findings suggest that SNPs of HCY metabolic enzymes influence the progression of ovarian cancer and hyperhomocysteinemia through genetic and epigenetic mechanisms.
Association of Perioperative Red Blood Cell Transfusion and Newly Initiated Renal Replacement Therapy Following Heart Valve Surgery
Authors: Lee Seung Hyong , Lee Jun Ho , Shin Yong Hoon , Kim Ji Eon , Jung Jae Seung , Son Ho Sung , Kim Hee-Jung 
Published: December 2024

Abstract

Perioperative red blood cell (RBC) transfusion is frequently required in heart valve surgery but is associated with immunologic complications and potential renal issues. Limited data exist on the link between perioperative RBC transfusions and the risk of end-stage renal disease (ESRD) requiring renal replacement therapy (RRT). This study evaluated the association between perioperative RBC transfusion and new RRT initiation post-heart valve surgery using data from the National Health Insurance Service (NHIS) database (2003–2019). Patients were grouped by transfusion volume: 0 units (no transfusion), 1 unit, or ≥ 2 units. The primary outcome was new RRT initiation. Among 27,562 patients (45.3% female; mean age 62.1 ± 12.3 years), 85.1% received transfusions. Incidence of RRT per 100 person-years was 0.52 for no transfusion, 0.69 for 1 unit, and 0.93 for ≥ 2 units. Multivariable Cox analysis revealed a dose-dependent association, with hazard ratios (HR) of 1.449 (1 unit; 95% CI: 1.042–2.015, p = 0.027) and 1.680 (≥ 2 units; 95% CI: 1.215–2.324, p < 0.002). Baseline eGFR < 30 mL/min/1.73 m² was also a significant risk factor (HR: 1.717, p = 0.05). This dose-dependent association emphasizes the need for optimized transfusion strategies to mitigate long-term renal risks in heart valve surgery patients.
Enhanced P-wave velocity imaging by marine controlled-source seismic surveys with Distributed Acoustic Sensing
Authors: Fukushima SHUN , Shinohara Masanao , Yamada Tomoaki , Hino Ryota , Azuma Ryosuke , Ito Yoshihiro , Yamashita Yusuke , Takano Hiroki 
Published: December 2024

Abstract

P-wave velocity (Vp) structures in marine areas have been estimated using marine controlled-source seismic surveys and refraction methods. The spatial resolution of Vp structures depends on the number of receivers and shots. In marine environments, Vp resolution is often limited due to the sparse distribution of ocean bottom seismometers (OBSs). Recently, the use of distributed acoustic sensing (DAS) has enabled high-resolution strain measurements over long distances, with station density along DAS cables being much higher than that of OBSs. Applying seismic control surveys and refraction methods to DAS data can enhance the horizontal resolution of Vp structures. This study demonstrates that seismic refraction using DAS data is effective for imaging shallow Vp structures with high spatial resolution. A seismic survey was conducted using R/V Hakuho-maru, controlled sources, and DAS and OBS measurements along a seafloor optical fiber cable off Sanriku, Japan. Strong lateral heterogeneities in the Vp structure were observed, due to dense DAS data. The 2-D Vp structure obtained from the DAS data showed good agreement with the seismic reflection profile. This experiment highlights the unique advantages of DAS data over OBSs for seismic refraction.
Association between recurrence risk perception and health behavior in patients with recurrent ischemic stroke in China: The mediation effect of Self-efficacy
Authors: Zhang Huan , Wu Yao , Cai Shaona , Yuan Lifang , Tian Xingyin , Li Yingying 
Published: December 2024

Abstract

Background：This study aims to explore the relationship between recurrence risk perception and health behavior in patients with recurrent ischemic stroke, and the mediating effect of self-efficacy between recurrence risk perception and health behavior.
Method：This cross-sectional study was conducted from May 2023 to November 2023 in China on 280 recurrent ischemic stroke patients . Data on sociodemographic and clinical characteristics, the Recurrence Risk Perception Scale for Stroke Patients, the General Self-Efficacy Scale, and the Health behavior Scale of Stroke Patient were included in this study.
Result：There were 266 valid questionnaires (95% effective recovery rate). In correlation analysis, high recurrence risk perception and high self-efficacy were significantly associated with better levels of health behavior (P<0.05). In the multiple linear regression model, after controlling for sociodemographic variables and clinical characteristics information in the t/F test (P<0.05), it was found that recurrence risk perception and self-efficacy were significant predictors of health behavior, and that exercise status, Course of Disease, and family history of stroke in the sociodemographic and clinical characteristics information in the present study had a significant effect on health behavior (P<0.05). Self-efficacy partially mediated the relationship between perceived risk of recurrence and health behavior (95% CI 0.102 to 0.334), with the mediating effect accounting for 37.3% (0.202/0.541)of the total effect.
Conclusion：Recurrence risk perception and self-efficacy were influential factors in promoting health behavior. In addition, the effect of recurrence risk perception on health behavior was mediated by self-efficacy.
Bilateral Continuous Thoracic Paravertebral Block Versus IV Fentanyl Infusion for Perioperative Analgesia in Patients Undergoing Cardiac Surgery Through Median Sternotomy
Authors: Kassim Dina , Rashwan Samaa , Mohamed Youssef , Omar Ihab , El-Shaarawy Ahmed 
Published: December 2024

Abstract

Background
In patients who are undergoing open cardiac surgery, thoracic paravertebral block (TPVB) is advantageous for pain management. Our objective was to compare this type of block with intravenous Fentanyl analgesia in those patients.
Methods
This prospective, randomized study included 44 patients who underwent open heart surgery through a median sternotomy. Participants were randomly divided into two equal groups (each with 22 subjects). Group Ⅰ (Fentanyl group) (n = 22): received fentanyl infusion 2µg/kg/h after placing the endotracheal tube and stopped at the end of surgery. Group Ⅱ (TPVB group) (n = 22): Bilateral thoracic paravertebral catheters were inserted preoperative. Before induction, there was a maximum of 20 ml per side for each catheter, which received a bolus dose of 0.3ml/kg 0.25% bupivacaine. Continuous infusion of 0.25% bupivacaine at a rate of 0.1 ml/kg/h was administered through each catheter following intubation. The infusion was subsequently discontinued at the conclusion of the procedure.
Results
Critical-Care Pain Observation Tool (CCPOT) was lower in patients of group Ⅱ (TPVB group) than in patients of group Ⅰ (fentanyl group); in group Ⅱ at 8hs, 12hs, and 24hs postoperative were 2,3,3 respectively, whereas median CCPOT in group Ⅰ at 8hs, 12hs, and 24hs postoperative were 3,4,4 respectively (p value < 0.05). In comparison to group Ⅰ, there was a substantial decrease in the intraoperative fentanyl requirements of group Ⅱ (343.6 µg ± 44.1 and 926.8 µg ± 117.4) (p-value < 0.05). In comparison to group I (9 mg 2), the TPVB group's postoperative opioid requirements were significantly lower (6.2 mg 2.3). P-value was less than 0.05.
Conclusion
Compared with IV fentanyl infusion, Bilateral continuous thoracic paravertebral block was determined to be more effective in the operative and postoperative analgesia of patients who underwent heart surgery via median sternotomy.
Enhanced Cytotoxicity against Cholangiocarcinoma by Fifth-Generation Chimeric Antigen Receptor T Cells Targeting Integrin αvβ6 and Secreting Anti-PD-L1 scFv
Authors: Phanthaphol Nattaporn , Somboonpatarakun Chalermchai , Suwanchiwasiri Kwanpirom , Yuti Pornpimon , Sujjitjoon Jatuporn , Baillie George S. , Junking Mutita , Yenchitsomanus Pa-thai 
Published: December 2024

Abstract

Cholangiocarcinoma (CCA) is a fatal bile duct cancer with high resistance and recurrence rates, with only one fifth of patients eligible for surgical treatment. The disease resists standard chemotherapy and often relapses. Chimeric antigen receptor (CAR) T cell therapy has shown promise for hematological malignancies but faces challenges in solid tumors due to resistance mechanisms like PD-L1 expression, which tumors use to evade the immune system. To address this challenge, we developed fifth-generation CAR T cells targeting integrin αvβ6 that also secrete anti-PD-L1 single-chain variable fragments (scFvs) to target both tumor cells and the PD-1/PD-L1 pathway. We examined integrin αvβ6 and PD-L1 expression in CCA cell lines and engineered T cells to express either fourth-generation CAR T cells targeting integrin αvβ6 (A20 CAR4 T cells) or fifth-generation CAR T cells with anti-PD-L1 scFv secretion (A20 CAR5 T cells). In vitro, A20 CAR5 T cells exhibited less exhaustion and superior long-term functionality compared to A20 CAR4 T cells. In 3D spheroid models of CCA, A20 CAR5 T cells demonstrated enhanced antitumor activity and better infiltration into the spheroid core. These findings suggest that A20 CAR5 T cells have significant potential and warrant further in vivo studies and clinical trials.
Incarceration history and HIV testing among people who inject drugs in the Boston metro area: a pooled cross-sectional study
Authors: Bovell-Ammon Benjamin J. , Onofrey Shauna , Kimmel Simeon D. , Wurcel Alysse G. , Klevens Monina 
Published: December 2024

Abstract

Background
The persistent incidence of HIV among people who inject drugs (PWID) underscores the urgency for HIV prevention efforts to end the HIV epidemic. Little is known about the role carceral settings play as touchpoints for HIV testing in this population.
Methods
Secondary analysis of cross-sectional survey data of PWID in the Boston metro area from the 2015 and 2018 cycles of the National HIV Behavioral Surveillance (NHBS). Among self-reported HIV-negative participants, we examined incarceration and HIV testing histories and used a multivariable modified Poisson regression model to evaluate the association between incarceration history (main exposure) and past-year HIV testing (primary outcome).
Results
Among 957 participants, average age was 38.9 (SD 11.1) years, 70.1% were male, 15.2% were Hispanic (of any race), 8.4% were non-Hispanic Black, and 68.1% were non-Hispanic White. Regarding incarceration experiences, 43.5% of participants reported past-year incarceration, and 41.8% reported a history of incarceration but only prior to the past year. Among those with past-year incarceration, 23.4% said their last HIV test was done at a jail or prison. Adjusting for other characteristics, compared to no incarceration history, past-year incarceration (PR 1.39; 95% CI: 1.29, 1.49) and incarceration prior to the the past year (PR 1.19; 95%CI: 1.02, 1.38) were both associated with a greater prevalence of past-year HIV testing.
Conclusions
Among PWID, incarceration was very common and was a substantial source of HIV testing. However, more testing is still needed—in both community and carceral settings—to reach optimal testing rates in this key population.
Crop Row Detection for Agricultural Autonomous Navigation based on GD-YOLOv10n-seg
Authors: Tao Sun , Longfei Cui , Feixiang Le , Xinyu Xue 
Published: December 2024

Abstract

Accurate crop row detection is an important foundation for agricultural machinery to realize autonomous operation. In this paper, a real-time soybean-corn crop row detection method based on GD-YOLOv10n-seg with PCA fitting is proposed. Firstly, the dataset of soybean-corn crop row was established, and the image was labeled by line label. Then, an improved model GD-YOLOv10n-seg model was constructed by integrating GhostModule and DynamicConv into the YOLOv10n-segmentation model. The experimental results show that the improved model performs better in MPA and MiOU, and the model size is reduced by 18.3%. The crop row center line of the segmentation results is fitted by PCA, the fitting accuracy reaches 95.08%, the angle deviation is 1.75°, and the overall processing speed is 57.32FPS. This study can provide an efficient and reliable solution for agricultural autonomous navigation operations such as weeding and pesticide application under soybean-corn compound planting mode.
Guidelines for Seizure Prophylaxis in Patients Hospitalized withNon-Traumatic Intracerebral Hemorrhage:A Clinical Practice Guideline for Healthcare Professionals from the Neurocritical Care Society
Authors: Frontera Jennifer A. , Rayi Appaji , Tesoro Eljim , Gilmore Emily J. , Johnson Emily L. , Olson DaiWai , Ullman Jamie S. , Yuan Yuhong , Zafar Sahar , Rowe Shaun 
Published: December 2024

Abstract

Background
There is practice heterogeneity in the use, type and duration of prophylactic antiseizure medications (ASM) in patients hospitalized with acute, non-traumatic intracerebral hemorrhage (ICH).
Methods
We conducted a systematic review and meta-analysis assessing ASM primary prophylaxis in adults hospitalized with acute, non-traumatic ICH. The following Population, Intervention, Comparator and Outcome (PICO) questions were assessed: 1) Should ASM versus no ASM be used in patients with acute ICH with no history of clinical or electrographic seizures?; 2) If an ASM is used, should levetiracetam (LEV) or phenytoin/fosphenytoin (PHT/fPHT) be preferentially used?; and 3) If an ASM is used, should a long (> 7 days) versus short (≤ 7 days) duration of prophylaxis be used? The main outcomes assessed were early seizure (≤ 14 days), late seizures (> 14 days), adverse events, mortality, and functional and cognitive outcomes. We utilized Grading of Recommendations Assessment, Development and Evaluation (GRADE) methodology to generate recommendations.
Results
The initial literature search yielded 1,988 articles, and 15 formed the basis of the recommendations. PICO 1: While there was no significant impact of ASM on the outcomes of early or late seizure or mortality, meta-analyses demonstrated increased adverse events and higher relative risk of poor functional outcomes at 90 days with prophylactic ASM use. PICO 2: We did not detect any significant positive or negative effect of PHT/fPHT compared to LEV for early seizures or adverse events, though point estimates tended to favor LEV. PICO 3: Based on one decision analysis, quality-adjusted-life-years were increased with a shorter duration of ASM prophylaxis.
Conclusions
We suggest avoidance of prophylactic ASM in hospitalized adult patients with acute, non-traumatic ICH (weak recommendation, very low quality of evidence). If used, we suggest LEV over PHT/fPHT (weak recommendation, very low quality of evidence) for a short duration (≤ 7 days, weak recommendation, very low quality of evidence).
Effect of factors on the space between the posterior capsule and IOL
Authors: da longd , zhou zhengbing , liu xiaohong , hu ping , long jinyi , xv ling , zhou chuyi , ye yuhong , wu xue 
Published: December 2024

Abstract

Objects: to evaluate the effect of factors on the space between the posterior capsule and IOL.
Methods: A total of 126 patients were included in the study. All patients underwent AS-OCT examinations after surgery. The relationship between the posterior capsule-IOL (PC-IOL) adhesion range and various factors, including IOL type, follow-up period, axial length, and lens thickness, were analyzed.
Results : When the follow-up period was shorter than 1 week, the proportion of PC-IOL adhesion range greater than 1/2 in the ZA9003, softec, SN6ATX, and 525 groups was 66.7%, 78.9%, 25%, and 53.8%, respectively. When the follow-up period was longer than 1 month, these proportions increased to 75%, 100%, 40.8%, and 100%, respectively. In the multivariate logistic regression analysis, only the IOL types and follow-up period were statistically significant, while axial length and lens thickness were not statistically significant.
Conclusion:The follow-up period and IOL types affect the space between the IOL and the posterior capsule, whereas axial length and lens thickness do not. Among the four IOL types studied, the softec IOL has the strongest adhesive power, while the SN6ATX IOL has the weakest.
Prognostic Value of Fluid and Cellular Monitoring in Septic Shock using Bioelectrical Impedance Analysis
Authors: Cha Susie , Diaz Jesse , Szerlip Harold 
Published: December 2024

Abstract

Background: Septic shock requires careful fluid management to optimize patient outcomes. However, traditional fluid assessment methods are often invasive and lack accuracy. This study explores the application of bioelectrical impedance analysis (BIA) to monitor fluid status and cellular integrity in septic shock patients during their first week in the intensive care unit (ICU).
Methods: This study enrolled 25 adult septic shock patients requiring vasopressors and mechanical ventilation within 24 hours of ICU admission (11 survivors, 14 non-survivors). Serial BIA assessments were performed on Days 0,3 and 7 and the daily rates of change were calculated between consecutive measures for fluid volume (total body water [TBW], extracellular water [ECW]), fluid distribution (the ECW/TBW ratio), and cellular integrity (reactance). Hierarchical clustering was used to identify similarity patterns among BIA parameters.
Results: During the initial period (Days 0-3), no difference in TBW was observed between survivors and non-survivors. However, survivors maintained a stable ECW/TBW ratio (-0.05%) and reactance (-0.10%), while non-survivors exhibited an increased ECW/TBW ratio (+2.73%, p<0.01) and decreased reactance (-9.42%, p<0.05). As the week progressed (Days 3-7), survivors showed signs of recovery with reduced TBW (-1.77%) and increased reactance (+3.50%). Meanwhile, non-survivors continued to deteriorate, with increased TBW (+1.27%, p<0.01), a further rise in ECW/TBW ratio (+0.25%, p=0.06), and decreased reactance (-4.33%, p<0.05).
Hierarchical clustering analysis distinguished two sets of physiological characteristics measured by BIA: one reflecting fluid status and another reflecting cellular health. The association of the ECW/TBW ratio with these clusters varied by outcome group. The ECW/TBW ratio clustered with cellular health parameters in survivors but with fluid status parameters in non-survivors.
Conclusions: This study shows that using BIA to monitor fluid volume, distribution, and cellular health provides clinicians with a comprehensive tool for monitoring patient progression in septic shock during resuscitation. By analyzing BIA parameters in a collective manner, this study offers new insights into the changes in fluid status and potential underlying factors driving these shifts. This approach not only improves the prognostic accuracy but also allows for more informed decisions on treatment efficacy and patient management through a noninvasive, bedside method. This approach may improve prognostic accuracy and could allow for more informed decisions on treatment efficacy and patient management through a noninvasive, bedside method.