bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of the pacemaker channel HCN4 in excitatory interneurons in the dorsal horn of the murine spinal cord
Authors: Nakagawa Taku , Yasaka Toshiharu , Nakashima Noriyuki , Takeya Mitsue , Oshita Kensuke , Tsuda Makoto , Yamaura Ken , Takano Makoto 
Published: September 2020

AbstractIn the central nervous system, hyperpolarization-activated, cyclic nucleotide-gated (HCN1–4) channels have been implicated in neuronal excitability and synaptic transmission. It has been reported that HCN channels are expressed in the spinal cord, but knowledge about their physiological roles, as well as their distribution profiles, appear to be limited. We generated a transgenic mouse in which the expression of HCN4 can be reversibly knocked down using a genetic tetracycline-dependent switch and conducted genetically validated immunohistochemistry for HCN4. We found that the somata of HCN4-immunoreactive (IR) cells were largely restricted to the ventral part of the inner lamina II and lamina III. Many of these cells were either parvalbumin- or protein kinase Cγ (PKCγ)-IR. By using two different mouse strains in which reporters are expressed only in inhibitory neurons, we determined that the vast majority of HCN4-IR cells were excitatory neurons. Mechanical and thermal noxious stimulation did not induce c-Fos expression in HCN4-IR cells. PKCγ-neurons in this area are known to play a pivotal role in the polysynaptic pathway between tactile afferents and nociceptive projection cells that contributes to tactile allodynia. Therefore, pharmacological and/or genetic manipulations of HCN4-expressing neurons may provide a novel therapeutic strategy for the pain relief of tactile allodynia.
YTHDF2, a protein repressed by miR-145, regulates proliferation, apoptosis, and migration in ovarian cancer cells
Authors: Li Jie , Wu Lei , Pei Meili , Zhang Yun 
Published: September 2020

AbstractRNA methylation can reverse the methylation modification at the RNA level, which is an extremely important epigenetic modification. The function and mechanism of YTHDF2, as a reader of m6A modification, in epithelial ovarian cancer (EOC) have not been elucidated so far. This study aimed to investigate how YTHDF2 and miR-145 modulated EOC progression through m6A modification. It demonstrated that YTHDF2 was significantly upregulated in EOC tissues compared with normal ovarian tissues. Further functional studies confirmed that YTHDF2 significantly promoted the proliferation and migration of EOC cell lines and reduced the global 6-methyladenine (m6A) mRNA levels. Next, the expression levels of miR-145 and YTHDF2 were found to be inversely correlated in ovarian cancer tissues and cells, and YTHDF2 was the direct target gene of miR-145. A crucial crosstalk occurred between miR-145 and YTHDF2 via a double-negative feedback loop. The overexpression of YTHDF2 rescued miR-145-induced reduction of the proliferation and migration of EOC cells. Hence, YTHDF2 and miR-145, as two crucial m6A regulators, were involved in the progression of EOC by indirectly modulating m6A levels. The findings of this study on YTHDF2 and miR-145 might provide new insights into carcinogenesis and new potential therapeutic targets for EOC.
Total colonic aganglionosis and cleft palate in a newborn with Janus-cysteine 618 mutation of RET proto-oncogene: a case report
Authors: Schierz Ingrid Anne Mandy , Cimador Marcello , Giuffrè Mario , Aiello Claudia Maria , Antona Vincenzo , Corsello Giovanni , Piro Ettore 
Published: September 2020

Abstract
Background
Hirschsprung disease, the most important congenital colonic dysmotility in children results from neural crest migration, differentiation, proliferation, or apoptosis defects where the rearranged during transfection (RET)-Protooncogene pathway has a central role. Although palatal and retinal anomalies in the context of chromosomopathies and some mono−/oligogenic syndromes are reported associated with Hirschsprung disease the role of inactivating RET mutations in these cases is not clarified.

Case presentation
We report on a dysmorphic newborn with cleft palate and palatal synechia, who showed intestinal obstruction after 24 h of life. Transient ileostomy and surgical biopsies were performed to diagnose aganglionosis of the colon and last ileal loop. No chromosomal anomalies or copy number variations were found. We identified a paternal heterozygous germline mutation c.1852 T > C, which results in the substitution of cysteine by arginine in the RET-receptor tyrosine kinase (p.C618R mutation). There was no family history of Hirschsprung disease, but the father underwent surgery for medullary thyroid carcinoma and was affected by retinal dystrophy.

Conclusions
The occurrence of Hirschsprung disease and carcinoma shows how a single mutation may be responsible for adverse effects: gain and loss of function of the same receptor. Furthermore, it would be interesting to study its dual role in face and retina embryology, and to extend targeted investigations of RET hotspots in these developmental abnormalities to facilitate counselling, follow-up, and tumor prevention. Complex surgical procedures and genetic testing as well as socio-economic impact are a challenge for familiar compliance.
Assessment of cyclic fatigue resistance of Protaper Next and WaveOne Gold in different kinematics
Authors: Ismail Amira Galal , Galal Manar , Roshdy Nehal Nabil 
Published: September 2020

Abstract
Background
The purpose of the current study was to inspect and compare the influence of applying continuous rotation and reciprocation motions on the cyclic fatigue resistance of Protaper Next (PTN) file (X2) and WaveOne Gold (WOG) Primary file in simulated canals. Twenty Protaper Next files(X2) and 20 WaveOne Gold Primary files were included in this study. A cyclic fatigue testing device was employed to test the cyclic resistance of each file in different motions. The testing device has an artificial custom-made stainless-steel canal with a 60° angle of curvature and a 2-mm radius of curvature. The files were randomly divided into 4 groups; group 1: PTN in continuous rotation, group 2: PTN in reciprocation, group 3: WOG in continuous rotation and group 4: WOG in reciprocation. All the instruments were rotated until fracture occurred, and the time to fracture was recorded in seconds using a digital chronometer. The number of cycles to fracture (NCF) was calculated. The data were analyzed statistically (p < .05).

Results
Results represented that when using either continuous rotation motion or reciprocating motion, WOG files showed a significantly longer time until failure than PTN files (p < 0.001). The time till fracture increased significantly, when using both types of files with a reciprocating filing motion.

Conclusion
Within the confinement of this study, WOG file in reciprocation showed higher cyclic fatigue resistance than PTN in both continuous rotation and reciprocating motion. The reciprocating motion enhances both files behavior in terms of cyclic fatigue resistance.
Relationship between serum 25-hydroxyvitamin D, total antioxidant capacity and pneumonia incidence, severity and outcome in Nigerian children
Authors: Kuti Bankole Peter , Akinwumi Alex Ifeoluwa , Kuti Demilade Kehinde , Amoo Kazeem Olanrewaju 
Published: September 2020

Abstract
Background
The pathologic basis of childhood community-acquired pneumonia (CAP) involves the generation of reactive oxygen species by immune cells leading to cellular damage and lung congestion. Serum antioxidants and vitamin D with immunomodulatory properties therefore hold prospects in the prevention and management of pneumonia in children. This case–control study set out to compare the serum 25-hydroxyvitamin D (25-OHD) and total antioxidant capacity (TAC) in Nigerian children with CAP and age- and sex-matched controls and to relate these parameters with pneumonia severity and outcome—length of hospital stay (LOH).

Results
A total of 160 children (80 each for CAP and controls) were recruited. The median (IQR) age was 1.8 (0.6–4.0) years, male:female 1.7:1, 63 (78.8%) and 11 (13.8%) of CAP group had severe pneumonia and parapneumonic effusions, respectively. Serum 25-OHD (33.8 (18.3) ng/ml vs. 41.9 (12.3) ng/ml; p = 0.010) and TAC (6.1 (4.4–8.1) ng/dl vs. 7.2 (4.7–17.5) ng/dl; p = 0.023) were lower in children with CAP than controls. Lower serum 25-OHD was observed in severe than non-severe pneumonia (30.5(17.1) ng/ml vs. 46.3 (17.6) ng/ml; p = 0.001) but LOH did not correlate with serum 25-OHD and TAC.

Conclusion
Children with CAP had lower serum vitamin D and antioxidants than controls, and severe pneumonia was significantly associated with suboptimal serum vitamin D. They however were not related to pneumonia outcome. Optimal serum vitamin D and antioxidants may play a role in reducing the incidence of childhood CAP in Nigerian children.
Optimization of prediction of morphological disorders of skeletal muscles in experimental acute ischemia-reperfusion on the basis of combined changes in lipid peroxidation and antioxidant protection by neural network clustering
Authors: Selskyy P. R. , Vastyanov R. S. , Klishch I. M. , Televiak A. T. , Volska A. S. , Kurylo Kh. B. , Selskyy B. P. 
Published: September 2020

The paper proposes a method for optimizing the prediction of the development of morphological disorders of skeletal muscle in experimental acute ischemia-reperfusion based on combined changes in lipid peroxidation and antioxidant protection. The approach is based on the use of neural network clustering.The purpose of the study – to propose a method for optimizing the prediction of the severity of morphological disorders in experimental acute ischemia-reperfusion on the basis of combined changes in lipid peroxidation and antioxidant protection by neural network clustering.The experimental model of ischemic-reperfusion lesion was represented by five groups of rats with reperfusion terms of 1 and 2 hours, 1 day, 7 and 14 days (18 animals each). Acute limb ischemia-reperfusion was simulated by applying SWAT rubber tourniquets on the hind right limb of animals for two hours under thiopental-sodium anesthesia. Histological examination was performed at the Department of Pathological Anatomy with a sectional course and forensic medicine of I. Horbachevsky Ternopil National Medical University of Ministry of Health of Ukraine according to generally accepted methods. Determination of lipid peroxidation and antioxidant protection in the blood serum of each experimental group of rats was performed by the Central Research Laboratory of I. Horbachevsky Ternopil National Medical University of Ministry of Health of Ukraine. For in-depth analysis and clustering of the indicators of the studied groups in order to optimize the prediction of the course of ischemic-reperfusion lesion, a neural network approach was used using the NeuroXL Classifier add-in for Microsoft Excel. The greatest prognostic value in relation to the severity of morphological disorders in the early reperfusion period according to the data of neural network clustering have combined changes in the level of TBA-active products and catalase.
Psychiatric aspect of most common used cardiological drugs
Authors: Cholewa Krystian , Wysokińska Olga , Siwek Michał , Kozyra Magdalena , Rudziński Gracjan , Milanowska Joanna 
Published: September 2020

Introduction: The count of psychiatric patients is growing. The same trend it is observed among cardiovascular diseases and metabolic like diabetes. Many people take drugs which may affect on mental state. On the one hand that drugs could have pleotropic mechanism of action which are beginning to use in treatment of psychiatry. The other hand is showed undiscovered field of impact on brain working. Methods and Aim: Shown the psychiatric aspect, like side effects and unusual use, of the most used cardiological drugs. To do this, research of articles was done with the help of deta bases such as PubMed and Google Scholar.Results: The most used cardiological drugs are beta blockers, calcium canal blockers, ACE inhibtors, ARBs, nitrats, cilostazol, amiodarone and clonidine In this article it was shown current known about those drugs like mechanism of action and a report of psychiatric clinical trials. Beta-blockers are the most commonly used group of cardiological drugs as the one that significantly affects mental health. Their therapeutic importance has been noted in diseases such as: PTSD or stage fright. It was also long believed that they could make depression worse, but the latest research strongly contradicts this. A positive effect in the treatment of depression can also be obtained by usage of inhibitors of the RAA. Calcium channel blockers are another group of cardiac drugs that are important in the regulation of mood in bipolar disorder. To sum up, a large proportion of cardiac drugs can influence the flow of psychiatric diseases, and the use of them may be clinically desirable in patients with both - cardiac and psychiatric problems.
Role of the gut microbiota in human health
Authors: Chałupnik Aleksandra , Chilimoniuk Zuzanna , Sobstyl Anna , Dobosz Maciej , Borkowska Aleksandra , Wieteska Małgorzata 
Published: September 2020

Introduction: The human gut microbiota has become the subject of extensive research in recent years and the knowledge of the species inhabiting the gut is growing rapidly. Microbiota plays an important role in human health and diseases. It takes part in obtaining and storing energy, as well as in metabolic functions.The aim of the study: The purpose of the study is to collect and review scientific publications about the impact of gut microbiota on human health.Material and method: The paper uses standard criteria as the research method. The articles used to create the reviews were published on the PubMed and Google Scholar platforms.Description of the state of knowledge: Numerous studies suggest that composition of intestinal microbiota has impact on the long-term functioning of the human organism. Depressive disorders appear to be associated with changes in the microbiota. In depressed patients, fewer bacteria of the type Bacteroidetes and higher level of Alistipes are found. A few studies have demonstrated a link between gut microbial signatures and hypertension. Studies have shown that T2DM patients may have an altered composition of gut microbiota, mainly a decreased Bacteroidetes/ Firmicutes ratio and decrease in some functional bacteria when compared to healthy people. Intestinal microorganisms are also a key element in the proper functioning of the immune system by influencing the maturation process of the immune system's cells. Summary: To sum up, numerous studies indicate that abnormal composition of the intestinal microflora is the cause of not only faster development of metabolic syndrome, but also other diseases. It influences the development of circulatory, nervous or immune system diseases. Its abnormal composition is influenced by many factors such as diet, lifestyle or antibiotic therapy.
The SLR test – neurodynamics and biomechanics
Authors: Cichosz Michał , Jetwuch Monika , Kochański Bartosz , Kałużny Krystian , Zukow Xawery 
Published: September 2020

The SLR test (straight leg raise), often reffered to as Lasègue's test, is one of the most often used provocative manoeuvres among patients with the pain ailments of the lower back (LBP). For the first time it was described in 1880 by a Serbian doctor called Lazerevic and since then numerous versions od this test has developed (1).
According to the authors' own experience, despite its large popularity in the clinical practice, the practitioners' interpretation of the test is often incorrect and is entirely confined to indicating the results in the dichotomous scale with the determination of the pain level. Such interpretation of the test cannot indicate the source of the pain ailments reported by the patients, all the more become a reference point for the treatment strategy. This study focues on the basic rules of neurodynamics and biomechanics of the SLR test and its varieties- the dynamic test of tibal, peroneal and sural nerves, which should serve as a model for working with the LBP patients.
The use of Kinesiology Taping among people aged between 25 and 55 with the non-specific pain of spine in its lumbar and sacral part
Authors: Kochański Bartosz , Kałużny Krystian , Kałużna Anna , Cichosz Michał , Gałęcki Sławomir , Hagner-Derengowska Magdalena , Zukow Xawery 
Published: September 2020

INTRODUCTION. The phenomenon of the pain ailments of spine is very complex and increasingly common. The key aspect of the treatment is the appropriate choice of the therapy, which may be dependent on many factors i.e.: the cause of the ailments, the phase of the disease, the clinical sypmtoms, the general health condition of the patient, the functional disorders and the patient's abbilities.
AIM OF THE RESEARCH. The aim of the research is the assessment and the comparison of the effects of two methods of the treatment: 1) the therapy based on general exercises focused on the lumbosacral spine combined with Kinesiology Taping and 2) the therapy based on general exercises focused on the pain intensisty and on the life quality of people with chronic, non-specific pain of the lumbosacral spine among patients aged 25-55.
MATERIALS AND METHODOLOGY. The research was conducted in a group of 50 people with chronic spine pain in the lumbosacral area. They were randomly divided into two groups: 1) the study group, where the ligament application of Kinesiology Taping was used for seven days (KT+GE), 2) the control group: no appliation of Kinesiology Taping (GE). Both groups took general exercises commonly used in the treatment of non-specific pain ailments of the lumbosacral spine for seven days. Durring the assessment of the effectiveness of the therapy the NRS scale (Numerical Rating Scale)and the Oswestry Low Back Pain Disability Scale questionnaire were used.
RESULTS. At the end of the treatment in the KT+GE group the decrease of the average pain intensity was observed from 6,20 to 2,12 (median 6,00 vs 2,00). The decrease of the average pain intensity was also noted in the GE group from 6,20 to 3,48 (median 6,00 vs 4,00). The statistical analysis showed that both forms of the therapy had a statistically significant impact (p
Development of physical culture and sports in Krements and Volyn between the military period during the activity of Julian Kozlowski, professor of Kremenets Lyceum
Authors: Plevachuk Lubov , Grygus Igor , Małolepszy Eligiusz 
Published: September 2020

This article describes Professor Julian Kozlowski’s activity at Kremenets Lyceum. Being an excellent specialist, he raised physical culture and sports to a new level, which made Kremenets Lyceum one of the best sports centers in Volyn.
Here it is shown the importance of innovations, including the Dalton system, implemented at physical education classes in Kremenets Lyceum.
Due to Julian Kozlowski”s persistence, there were introduced mass sports tests for Ski Distinction and for State Sports Honors.
Thanks to his initiative, ski jumping, swimming pool on the river Ikva, stadium, tennis courts were built here. He also participated in the construction of gliding sport school on Sokolyna Mountain near Kremenets.
Professor Kozlowski gave lectures and courses to popularize physical culture and sports. He initiated various competitions and sports events.
Julian Kozlowski was a great patriot of Poland.
Coupled quantum molecular cavity optomechanics with surface plasmon enhancement: comment
Authors: Ashrafi Seyed Mahmoud , Taghadomi Narjes , Bahrampour Alireza , Malekfar Rasoul 
Published: September 2020

We have found an incorrect formula to estimate the root mean square (rms) amplitude of the molecular vibration in the molecular optomechanics systems reported by J. Liu et al. [Photon. Res. 5, 450–456 (2017)PRHEIZ2327-912510.1364/PRJ.5.000450]. In contrast with common optomechanical systems, the equilibrium position for molecular optomechanics systems used in the letter cannot be achieved by the equipartition theorem. Here, we achieve the effective temperature of molecules and then provide a corrected formula for the estimation of the rms amplitude of molecular motion. Using defined effective temperature into our new formula, we show that the minimal measurable force is (

F
≈
1.7
×

10

−
14

N

), which it is 1 order bigger than the result of the main paper, and it is also in accordance with numerical calculation through the Lindblad master equation.
Arlen & Harburg’s ‘Over the Rainbow’ (Oxford Keynotes), Walter Frisch (2017)
Authors: Buchman Andrew 
Published: September 2020

Review of: Arlen & Harburg’s ‘Over the Rainbow’ (Oxford Keynotes), Walter Frisch (2017)
New York: Oxford University Press, 146 pp.,
ISBN: 978-0-19046-734-0, p/bk, $15.95 (h/bk, $78)
Model Pengadaan Obat dengan Metode ABC VEN Di RS X Semarang
Authors: Wulandari Siska , Sugiarto Sugiarto 
Published: September 2020

Procurement of drugs at RS X was carried out unsystematically by the head of the RS Pharmacy Installation. This procurement causes the costs incurred by the Hospital to be unplanned and inefficient. The value of the use of active pharmaceutical supplies in 2016 with the number of pharmaceutical supplies of 2119 is worth Rp. 168,232,696 and the number of 2041 inactive supplies worth Rp. 533,260,268. The data shows the inefficiency of pharmaceutical supplies within 1 year which results in the use of budget funds less efficiently. The purpose of this study was to determine the role of the ABC VEN method as a basis for procurement systems in Hospital X. The type of research conducted is quantitative, pre experimental using descriptive analytical methods with analysis of ABC and VEN methods. Samples were taken as much as 500 from the data of drug use for 1 year in RS X Pharmacy Installation. The results of the study of 500 drug items with the ABC method found Group A 19.8%, Group B 40%, Group C 40.2%, the VEN method obtained group V 7.8%, Group E 88.8%, group N 3, 4%, with the ABC method VEN AV group 2%, AE 17.6%, AN 0.3%, BV 4.2%, BE 35%, BN 1.2%, CV 1.8%, CE 37%, CN 1.8%. The CE (C Essential) group is more due to its function as prevention and prevention of patient diseases. Before doing the ABC VEN method the inventory value is Rp. 6,188,183,555 and after the ABC VEN method was Rp. 5.833.095.547 with a difference of Rp. 355.089.998, (6.8%) of the total usage fee. The conclusion of this study is that the system used by the Pharmacy Pharmacy Installation has not been efficient, causing losses for hospitals. Difference in inventory value before and after the ABC VEN method is Rp. 355,089,998. Suggestions are changing the old system to a new system through prior socialization and regulation.