bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unmet supportive care needs and its relation to quality of life among adult acute leukaemia patients in China: a cross-sectional study
Authors: Yan Jie , Wang Ying , Chen Jingyi , Wang Chunfeng , Lin Yingchun , Hu Rong , Wu Yong 
Published: March 2020

Abstract
Background: Patients with acute leukaemia (AL) usually require prolonged periods of hospitalisation. The treatment and clinical symptoms may lead to patients’ supportive care needs (SCNs) not being met and impairs their quality of life (QoL). Studies on QoL and SCNs among AL patients are limited. This study aimed to identify the unmet SCNs and its relation to QoL of adult AL patients in China.Methods: This multicentre cross-sectional study recruited 346 participants to complete a self-developed questionnaire, detailing demographic information and disease-related variables. A 34-item Supportive Care Needs Survey (SCNS-SF34) was used to identify unmet SCNs, and the Functional Assessment of Cancer Therapy-Leukaemia (FACT-Leu) questionnaire measured patients’ QoL.Results: Unmet SCN rates for the 34 items ranged from17.6% to 81.7%. Patients’ needs were high for health systems and information, but low in the sexual domain. The results reveal nine factors associated with the unmet SCNs of adult AL patients, including marital status, original residence, age, education, occupation, other diseases, chemotherapy course, disease course, and treatment stage (p <0.05). The total score of the FACT-Leu negatively correlated with the SCNS-SF34 in the physical/daily living (r = - 0.527, p <0.01), psychological (r = - 0.688, p <0.01), sexual (r = - 0.170, p <0.01), patient care and support (r = - 0.352, p <0.01), and health systems and information (r = - 0.220, p <0.01) domains.Conclusions: Adult AL patients exhibit a high demand for unmet SCNs, especially in the domain of health systems and information. There was a significant association between patients’ unmet SCNs and QoL. Future research should develop tailored interventions to address the unmet SCNs of adult AL patients, to further improve their QoL.
Is oral lichen planus a risk factor for peri-implant diseases? A systematic review and meta-analysis
Authors: Xiong Xiaoqin , Yu Ting , Xu Tiantian , Wang Xinhong , Qin Wenguang , Gang Luo 
Published: March 2020

Abstract
Background : To evaluate whether oral lichen planus (OLP) is a risk factor for peri-implant diseases (PIDs) with a systematic review and meta-analysis. Methods : Six electronic databases including Medline, Web of Science, etc. were searched. Included studies are observational human studies written in English. Population of interest were those with/without OLP who received dental implant treatment. Follow-up time after implantation is from one month to 20 years. The quality of the included literature regarding risk of bias and methodology was assessed with Newcastle-Ottawa Scale or the Agency for Healthcare Research and Quality. The data involving exposure (OLP), primary outcomes (implants having PIDs) and secondary outcomes (probing depth/PD, bleeding on probing/BOP and bone loss/BL) and potential confounders were extracted. Heterogeneity was assessed by I² tests. Dichotomous data were expressed as risk ratio (RR) and 95% confidence interval (CI) which were calculated with a fixed effect model. Results : Of 139 literatures, two studies were enrolled and evaluated as high quality, which totally contained 68 participants receiving 222 (OLP vs. non-OLP, 112 vs. 110) implants with 12 to 120-month follow-up time. Proportions of implants with PIDs between OLP and non-OLP groups were as follows: 19.6% (22/112) vs. 22.7% (25/110) for PIM; 17.0% (19/112) vs. 10.9% (12/110) for PI. Meta-analysis found no recognizable difference in number of implants with PIDs (PI: RR = 1.49, 95% CI 0.77-2.90, P = 0.24; PIM:RR = 0.88, 95% CI 0.53 -1.46, P = 0.61; PIDs: RR = 1.08, 95% CI 0.75 -1.55, P = 0.68) or BOP (RR = 0.90, 95% CI: 0.70-1.15, P = 0.40) between OLP and non-OLP groups. Conclusions : Available literature regarding the effects of OLP on PIDs remains very limited. Existing evidence seems not support OLP as a suspected risk factor for PIDs. Large-scale prospective trials are required to test the findings. Keywords : dental implants; peri-implant diseases; oral lichen planus; systematic review; meta-analysis.
Association of Root Surface Caries with Periodontal Parameters in Type 2 Diabetes Patients
Authors: Zhong Sulan , Li Duo , Liang Minlu , Tong Fangli , Li Xiaomin , Chen Lei 
Published: March 2020

Abstract
Background: Mounting evidence has demonstrated a reciprocal association between diabetes mellitus and periodontal diseases, characterized by loss of alveolar bone, gingival recession and root exposure. Recently, studies showed that type 2 diabetes patients were at high risk to development root caries. This essay aims to clarify the relationship between root surface caries (RC) and periodontal parameters in diabetes patients, as well as evaluate relevant risk factors in Type 2 diabetes mellitus (T2DM). Materials and Methods: A total of 132 qualified adult patients with T2DM and periodontitis were recruited to this study. Radiographic data were collected to calculate alveolar bone loss (ABL). Subjects were assigned to three groups according to tertiles of ABL to make a comparison. Decayed and/or ﬁlled teeth (DFT) were calculated for both coronal and root surface as the caries indices of the remaining teeth. The plaque index (PLI), gingival recession (GR), probing depth (PD) at six sites per tooth, number of missing teeth, number of retained root remnants, and number of teeth with furcal lesions were recorded during clinical examinations. Blood analyses were carried out for glycated hemoglobin (HbA1c), fasting glucose. Correlation analysis, analysis of covariance (ANCOVA), and logistic regression were used to analyse the data. Results: By analyzing covariance, the research found that subjects with increased ABL have significantly higher numbers of missing teeth, root surface caries DFT, teeth with furcal lesions ( P <0.05). There is a significant positive correlation between root surface caries DFT and ABL, GR, coronal caries DFT ( P <0.05), respectively. ABL, coronal caries and age became significant predictor variables for Root Surface DFT (≥3)) ( P <0.05). Conclusion: Root surface caries were associated with ABL, GR and coronal caries in periodontitis with T2DM as well as ABL, coronal caries and age became risk factors for predicting the occurence of RC. Key Words: Association, Root Caries, Diabetes mellitus, Periodontitis
Hepatitis C viraemic and seroprevalence and risk factors for positivity in northwest Cambodia: a household cross-sectional serosurvey
Authors: Lynch Emily , Falq Gregoire , Sun Chhorvy , Bunchhoeung PharmD Tek , Huerga Helena , Loarec Anne , Dousset Jean-Phillipe , Marquardt Tonia , Paih Mickael Le , Maman David 
Published: March 2020

Abstract
BACKGROUND Despite a dramatic reduction in HCV drug costs and simplified models of care, many countries lack important information on prevalence and risk factors to structure effective HCV services.METHODS A cross-sectional, multi-stage cluster survey of HCV seroprevalence in adults 18 years and above was conducted, with an oversampling of those 45 years and above. 147 clusters of 25 households were randomly selected in two sets (set 1=24 clusters ≥18; set 2=123 clusters, ≥45). A multi-variable analysis assessed risk factors for sero-positivity among participants ≥45. The study occurred in rural Moung Ruessei Health Operational District, Battambang Province, Western Cambodia.RESULTS A total of 5,098 individuals and 3,616 households participated in the survey. The overall seroprevalence was 2.6% (CI95% 2.3-3.0) for those ≥18 years, 5.1% (CI95% 4.6-5.7) for adults ≥ 45 years, and 0.6% (CI95% 0.3-0.9) for adults 18-44. Viraemic prevalence was 1.9% (CI95% 1.6-2.1), 3.6% (CI95% 3.2-4.0), and 0.5% (CI95% 0.2 - 0.8), respectively. Men had higher prevalence than women: ≥18 years male seroprevalence was 3.0 (CI95% 2.5 - 3.5) versus 2.3 (CI95% 1.9 - 2.7) for women. Knowledge of HCV was poor: 64.7% of all respondents and 57.0% of seropositive participants reported never having heard of HCV. Risk factor characteristics for the population ≥45 years included: advancing age (p<0.001), low education (higher than secondary school OR 0.7 [95% CI 0.6-0.8]), any dental or gum treatment (OR 1.6 [95% CI 1.3-1.9]), historical routine medical care (medical injection after 1990 OR 0.7 [95% CI 0.6-0.9]; surgery after 1990 OR 0.7 [95% CI0.5-0.9]), and historical blood donation or transfusion (blood donation after 1980 OR 0.4 [95% CI 0.2-0.9]); blood transfusion after 1990 OR 0.7 [95% CI 0.4-1.1]).CONCLUSIONS This study provides the first large-scale general adult population prevalence data on HCV infection in Cambodia. The results confirm the link between high prevalence and age ≥45 years, lower socio-economic status and past routine medical interventions (particularly those received before 1990 and 1980). This survey suggests high HCV prevalence in certain populations in Cambodia and can be used to guide national and local HCV policy discussion.
Dysregulation of autophagy-associated microRNAs in condyloma acuminatum
Authors: Wu Shi , Lu Dan , Zheng Xinkai , Xu Jin , Li Zhen , Deng Liehua , Hu Yunfeng 
Published: March 2020

Abstract
Objectives This study was designed to investigate the miRNAs that regulate the cell proliferation of condyloma acuminatum (CA) lesions and their targets.Methods The expression of Ki-67 in 26 CA patients compared with 10 healthy controls was assessed by immunohistochemistry. And the different miRNAs in 4 CA patients and 4 control cases were analyzed by bioinformatics. PCR was used to validate the expression of screened miRNA and its corresponding target genes.Results The expression of Ki-67 was abnormally increased in CA compared with healthy controls ( P <0.05). The comparison of the control group with the CA group revealed 81 differentially expressed miRNAs, of which 56 were downregulated and 25 were upregulated. Two of the differentially expressed miRNAs, miR-30a-5p and miR-514a-3p, are associated with cell proliferation and their target genes are autophagy-related protein (Atg) 5 and Atg12, and Atg3 and Atg12, respectively. PCR results showed that the expression levels of miR-30a-5p and miR-514a-3p were decreased in CA patients compared with healthy controls ( P <0.05), whereas the expression of Atg5, Atg12 and Atg3 was increased ( P <0.05). The expression of the autophagy proteins microtubule-associated protein 1 light chain 3 (LC3) and P62/SQSTM1 (P62) was abnormally increased in the local lesion tissue of the 26 patients with CA compared with the 10 healthy controls, as assessed by immunohistochemistry ( P <0.05).Conclusions Our results suggest that autophagy levels may be modulated by has-miRNA30a-5p and has-miRNA514a-3p in CA patients, leading to dysregulated cell proliferation.
Aberrant autophagy and proinflammatory cytokines may reduce endometrial decidualization: a essential role of obesity on infertility
Authors: Lv Ya-su , Wang Chen , Han Shuo , Li Ling-xiu , Li Yuan 
Published: March 2020

Abstract
Background: Obesity is associated with reproductive disorders and infertility. Autophagy is upregulated during decidualization, but is diminished in obesity models with impaired decidualization. Defects in autophagy are related to the occurrence of inflammatory diseases, but its role in endometrial decidualization with obese patients is unknown.Methods: The levels of autophagy-related factors (LC3B-II and Beclin 1), and autophagy-related proteins which have a correlation with endometrial decidualization (ATG3, ATG5, ATG7 and foxo1), as well as autophagy-related inflammatory cytokines in endometrium of lean and obese patients was assessed. We then investigated the the role of autophagic flux during decidualization in ‘obese’- vs ‘lean’- treated cells from matched patients.Results: Alteration in autophagic flux and the expression of autophagy-related genes revealed impaired autophagy in obese patients compared with lean. Autophagy-related proinflammatory cytokines were upregulated in the uterine cavity of obese patients, who also showed impaired decidualization.Conclusions: Obesity downregulates the expression of autophagy-related genes and impairs endometrial stromal cell autophagic flux, and induces inflammation. These alterations in autophagy are associated with increased activation of autophagy-related proinflammatory cytokines might decrease basic uterine receptivity.
Magnesium supply regulate leaf nutrition and plant growth of soilless cultured cherry tomato - interaction with potassium
Authors: Guan Xilin , Wang Xiaozhong , Liu Bin , Wu Changchun , Liu Chuanyun , Liu Dunyi , Zou Chunqin , Chen Xinping 
Published: March 2020

Abstract
Background: Magnesium (Mg) is essential to many plant physiological and biochemical processes; however, a quantitative understanding of how Mg nutrition affects the production, partitioning and utilization of photoassimilates is still lacking, especially for soilless culture system. We focused on the roles of Mg in yield formation and interactions with potassium (K) nutrition of cherry tomato. Cherry tomato yield, leaf Mg concentration, photosynthetic parameters, dry matter weight and K, Mg and calcium (Ca) uptake were investigated in two soilless experiments with seven Mg levels and five K levels.Results: Low (<1 mM) and high (>4 mM) Mg supply limited cherry tomato yield by decreasing 22.6-78.1% dry matter accumulation and 13.9-40.7% harvest index. The critical leaf Mg concentrations required for adequate photosynthate production in the first and second harvest periods were 4.67 and 5.52 g kg –1 , respectively. But over-supply of Mg disturbed leaf K and Ca concentrations, limiting the plant K and Ca content. Moreover, adjusting K concentrations in solution is a crucial factor influencing plant Mg functions, and therefore cherry tomato growth.Conclusion: Balanced Mg and K application increased Mg, K, and Ca uptake by cherry tomato, as well as Mg concentrations in leaves which could maintain a sustainable photosynthetic rate and plant dry matter formation.
Identification of fruit size associated quantitative trait loci featuring SLAF based high-density linkage map of goji berry (Lycium spp.)
Authors: Rehman Fazal , Gong Haiguang , Li Zhong , Zeng Shaohua , Yang Tianshun , Ai Peiyan , Pan Lizhu , Wang Ying , Huang Hongwen 
Published: March 2020

Abstract
Background: Goji (Lycium spp., 2n = 24) is a perennial woody plant bearing functional properties. Fruit size associated attributes are important for evaluating small-fruited goji berry and plant architecture. The domestication traits are regulated quantitatively in crop plants but fewer studies have attempted on genomic regions corresponding to the fruit traits.Results: In this study, we established high-resolution map using SLAF-seq for de novo SNPs detection, based on 305 F1 offspring derived from L. chinense and L. barbarum. This genetic map contained 3,495 SNP markers on 12 LGs, spanning 1,649.03 cM with 0.47 cM average interval. Female and male parents had sequencing depth of 72.43-fold and 60.43-fold, respectively, while 15.23-fold detected for individuals. Phenotype data were obtained for 2 years and included 3rd year data as an average. QTL mapping analysis resulted in 117 QTLs corresponding to all traits, of which 23 QTLs in 2 years and 6 QTLs in 3rd years were detected. 6 promising QTLs, qFW10-3.1, qFL10-2.1, qLL10-2.1, qLD10-2.1, qLD12-4.1 and qLA10-2.1 were discovered influencing fruit weight, fruit length and leaf size related attributes covering an interval ranged 13.74-76.61 cM on LG10 with peak LOD up to 14.21 and PVE 19.3%. Additionally, 3 QTLs, qFS-1, qFS-2 and qFF-1, targeting fruit sweetness and fruit firmness were also identified in this study. Strikingly, among stable QTLs, qFL10-2.1, was co-localized to qLL10-2.1, qLD10-2.1, qLA10-2.1 and harbored significantly similar markers distribution, while qLL10-2.1 was the major and stable QTL with LOD ranged from 8.71-14.21 and PVE 12.3-19.3%. As LG10 harbored most of the fruit and leaf size related QTLs, we might speculate that it could be a hotspot region regulating fruit size and plant architectures.Conclusions: This report highlighted that the high-density linkage map construction using SLAF-seq is an important means for profound QTL mapping approach. Substantially, we utilized highly saturated genetic map to find out genetic locus targeting fruit and leaf size related attributes under QTL mapping. Our results will shed light on domestication traits and further strengthen molecular and genetic underpinnings of goji berry. Moreover, these findings would facilitate to assemble reference genome, determining potential candidate genes and marker-assisted breeding.
Adjuvant Transarterial Chemoembolization does not Influence Recurrence-free and Overall survivals for Patients with Combined Hepatocellular Carcinoma and Cholangiocarcinoma after Curative Resection: a Propensity Score Matching Analysis
Authors: Liu Wei-Ren , Tian Meng-Xin , Tao Chen-Yang , Tang Zheng , Zhou Yu-Fu , Song Shu-Shu , Jiang Xi-Fei , Wang Han , Zhou Pei-Yun , Qu Wei-Feng , Fang Yuan , Ding Zhen-Bin , Zhou Jian , Fan Jia , Shi Ying-Hong 
Published: March 2020

Abstract
Background : The prognosis of patients with combined hepatocellular carcinoma and intrahepatic cholangiocarcinoma (CHC) is usually poor, and effective adjuvant therapy is ineffective making it important to investigate whether these patients may benefit from adjuvant transarterial chemoembolization (TACE). We aimed to evaluate the efficiency of adjuvant TACE for long-term recurrence and survival after curative resection before and after propensity score matching (PSM) analysis. Methods : In this retrospective study, of 230 patients who underwent resection for CHC between January 1994 and December 2014, 46 (18.0%) patients received adjuvant TACE. Univariate and multivariate regression analyses were used to identify the independent predictive factors of survival. Cox regression analyses and log-rank tests were used to compare overall survival (OS) and disease-free survival (DFS) between patients who did or did not receive adjuvant TACE. Results : A total of 230 patients (mean age 52.2±11.9 years; 172 men) were enrolled, and 46 (mean age 52.7±11.1 years; 38 men) patients received TACE. Before PSM, in multivariate regression analysis, γ-glutamyl transpeptidase (γ-GT), tumour nodularity, macrovascular invasion (MVI), lymphoid metastasis, and extrahepatic metastasis were associated with OS. Alanine aminotransferase (ALT), MVI, lymphoid metastasis, and preventive TACE (HR: 2.763, 95% CI: 1.769-4.314, p <0.001) were independent prognostic factors for DFS. PSM created 46 pairs of patients. After PSM, adjuvant preventive TACE was not associated with an increased risk of OS (HR: 0.911, 95% CI: 0.545-1.520, p =0.720) or DFS (HR: 3.345, 95% CI: 1.686-6.638, p =0.001). After PSM, the 5-year OS and DFS rates were comparable in the TACE group and the non-TACE group (OS: 22.7% vs 14.9%, respectively, p =0.75; DFS: 11.2% vs 14.4%, respectively, p =0.06). Conclusions : The present study identified that adjuvant preventive TACE did not influence DFS or OS after curative resection of CHC.
Predictive nomogram of subsequent liver metastasis after mastectomy or breast-conserving surgery in patients with nonmetastatic breast cancer
Authors: Yang Anli , Xiao Weikai , Zheng Shaoquan , Kong Yanan , Zou Yutian , Li Mingyue , Ye Feng , Xie Xiaoming 
Published: March 2020

Abstract
BACKGROUND Metastasis accounts for the majority of deaths in patients with breast cancer. Liver metastasis is reported common to breast cancer patients. The purpose of this study was to construct a nomogram to predict the likelihood of subsequent liver metastasis in patients with nonmetastatic breast cancer, thus high-risk patient populations can be prevented and monitored. METHODS A total of 1840 patients with stage I-III breast cancer were retrospectively included and analyzed. A nomogram was constructed to predict liver metastasis based on multivariate logistic regression analysis. C-index, calibration curve and decision curve analysis were used to evaluate the predictive performance of the model. RESULTS The nomogram included three variables related to liver metastasis: HER2 status (odds ratio (OR) 1.86, 95%CI 1.02 to 3.41; P =0.045), tumor size (OR 3.62, 1.91 to 6.87; P < 0.001) and lymph node metastasis (OR 2.26, 1.18 to 4.34; P =0.014). The C index in the training cohort was 0.699 and it was 0.814 in the validation cohort (691 patients). The nomogram was well-calibrated, with no statistical difference between the predicted and the observed probabilities. CONCLUSION We have developed and validated a robust tool enabled to predict subsequent liver metastasis in patients with nonmetastatic breast cancer. Distinguishing a population of patients at high risk of liver metastasis will facilitate preventive treatment or monitoring of liver metastasis.
Mode of Bacterial Killing Affects the Inflammatory Response and Associated Organ Dysfunctions in a Porcine E. coli Intensive Care Sepsis Model
Authors: Skorup Paul , Maudsdotter Lisa , Lipcsey Miklós , Larsson Anders , Sjölin Jan 
Published: March 2020

Abstract
Background Sepsis is often treated with penicillin-binding protein 3 (PBP-3) acting β-lactam antibiotics, such as piperacillin-tazobactam, cefotaxime and meropenem. They cause considerable bacterial structural changes and have in vitro been associated with an increased inflammatory response. In a clinically relevant large animal sepsis model our primary aim was to investigate whether bacteria killed by a PBP-3 active antibiotic has a greater effect on the early inflammatory response and organ dysfunction compared with corresponding amounts of live or heat-killed bacteria. A secondary aim was to determine whether the addition of an aminoglycoside could mitigate the cefuroxime-induced response. Method Killed or live Escherichia coli were administrated as a 3-hour (h) infusion to 16 healthy pigs in a prospective, placebo-controlled interventional experimental study. Cefuroxime was chosen as the PBP-3 active antibiotic and tobramycin represented the aminoglycosides. The animals were randomized to receive I) bacteria killed by cefuroxime, II) live bacteria, III) bacteria killed by heat or IV) bacteria killed by the combination of cefuroxime and tobramycin. Plasma endotoxin, tumor necrosis factor alpha, interleukin-6, interleukin-10, leukocytes and organ function were recorded at the start of the experiment and then hourly for 6 h. Results Differences in dynamics of concentration over time between the four treatment groups were found for the three cytokines (p<0.001). Animals receiving cefuroxime-killed bacteria demonstrated higher responses than those receiving live or heat-killed bacteria (p<0.01). The addition of tobramycin reduced the cefuroxime-induced responses (p<0.001). The cytokine responses were associated with leucocyte activation that was further associated with pulmonary dysfunction and increases in lactate (p<0.01). Conclusions In comparison with live or heat-killed bacteria, bacteria killed by a PBP-3 active antibiotic induced an increased inflammatory response associated with deteriorated organ and cellular function. The addition of an aminoglycoside to the PBP-3 active antibiotic reduced that response.