bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term prognostic counselling in people with multiple sclerosis using an online analytical processing tool
Authors: Kosch Ricardo , Schiffmann Insa , Daumer Martin , Lederer Christian , Scalfari Antonio , Galea Ian , Scheiderbauer Jutta , Rahn Anne , Heesen Christoph 
Published: October 2020

Background: Prognostic counselling is a sensitive issue in medicine and especially so in MS due to the highly heterogeneous disease course. However, people with MS (pwMS) seek prognostic information. The web-based ‘Evidence-Based Decision Support Tool in Multiple Sclerosis’ (EBDiMS) uses data of 717 patients from the London/Ontario cohort to calculate personalized long-term prognostic information. Objective: The aim of this study was to investigate the feasibility and effect of long-term prognostic counselling in pwMS using EBDiMS. Methods: Ninety consecutive pwMS were provided with personalized estimations of expected time to reach Expanded Disability Status Scale (EDSS) scores of 6 and 8 and time to conversion to secondary-progressive MS. Participants gave estimates on their own putative prognosis and rated the tool’s acceptability on six-step Likert-type scales. Results: Participants rated EBDiMS as highly understandable, interesting and relevant for patient–physician encounters, coping and therapy decisions. Although it provoked a certain degree of worry in some participants, 95% would recommend using the tool. Participants’ own prognosis estimates did not change significantly following EBDiMS. Conclusion: Long-term prognostic counselling using an online tool has been shown to be feasible in a clinical setting. EBDiMS provides pwMS with relevant, easy-to-understand, long-term prognostic information without causing relevant anxiety.
Major lower extremity amputations in a developing country: 10-Year experience at a tertiary medical center
Authors: Chahrour Mohamad A , Homsi Mouafak , Wehbe Mohammad R , Hmedeh Caroline , Hoballah Jamal J , Haddad Fady F 
Published: October 2020

Background Lower extremity amputation (LEA) is a major surgical procedure with a high risk of significant morbidity and mortality. The objective of this study was to describe mortality and functionality outcomes following this procedure in a developing country. Methods This is a retrospective study of all patients undergoing LEA for non-traumatic etiology between 2007 and 2017. Medical records were used to retrieve demographics, comorbidities, and perioperative complications of identified patients. Patients were contacted to follow-up on their medical, postoperative care, and ambulatory status. Mortality and postoperative functionality rates were analyzed. Results The study included 78 patients. Median follow-up duration was 24 months. Hypertension (81%) and diabetes (79%) were the most common comorbidities. Mortality rates at 30 days, 1, and 5 years were 10.3, 29.2, and 65.5%, respectively. Mortality was significantly associated with age > 70 at amputation ( p = 0.042), hypertension ( p = 0.003), chronic kidney disease ( p = 0.031), and perioperative sepsis ( p = 0.01). Only 1.6% of patients were discharged into a specialized care center, and only 27% of patients were ambulatory postoperatively, although 90.5% were fitted with a prosthesis. Conclusions Survival following major amputation in a developing country is currently comparable to more developed regions of the world. Major discrepancy seems to exist in ambulatory status following the procedure. Discharge placement policies should be properly set, and rehabilitation centers funding should be increased. Awareness may also be warranted to educate patients and families about the value and positive impact of rehabilitation centers.
Work-related ill- and well-being among Finnish sport coaches: Exploring the relationships between job demands, job resources, burnout and work engagement
Authors: Kaski Satu S , Kinnunen Ulla 
Published: October 2020

Objective This research examined the work-related ill- and well-being of Finnish sport coaches and the associations of job demands and resources with burnout and work engagement. Our study was based on the Job Demands-Resources (JD-R) model. The JD-R model proposes that job demands relate especially to burnout and job resources relate especially to work engagement. Methods Finnish coaches (N = 499) within top-level sports completed a questionnaire measuring burnout, work engagement, job demands and resources. Results The results of hierarchical regression analyses showed that burnout was explained with job demands, but also lack of job resources added the explanation rate almost equally. The most important single contributor to burnout was lack of positive challenge at work. Work engagement was explained by job resources, from which positive challenge at work together with mastery of work were the strongest contributors. In addition, decision demands contributed slightly to the explanation of work engagement. Conclusions The results suggest that in the work of sport coaches, job resources or lack of them are more essential than job demands to explain both burnout and work engagement.
Adjustment of Insulin Pump Settings in Type 1 Diabetes Management: Advisor Pro Device Compared to Physicians’ Recommendations
Authors: Nimri Revital , Oron Tal , Muller Ido , Kraljevic Ivana , Alonso Montserrat Martín , Keskinen Paivi , Milicic Tanja , Oren Asaf , Christoforidis Athanasios , den Brinker Marieke , Bozzetto Lutgarda , Bolla Andrea Mario , Krcma Michal , Rabini Rosa Anna , Tabba Shadi , Smith Lizl , Vazeou Andriani , Maltoni Giulio , Giani Elisa , Atlas Eran , Phillip Moshe 
Published: October 2020

Aims: To compare insulin dose adjustments made by physicians to those made by an artificial intelligence-based decision support system, the Advisor Pro, in people with type 1 diabetes (T1D) using an insulin pump and self-monitoring blood glucose (SMBG). Methods: This was a multinational, non-interventional study surveying 17 physicians from 11 countries. Each physician was asked to provide insulin dose adjustments for the settings of the pump including basal rate, carbohydrate-to-insulin ratios (CRs), and correction factors (CFs) for 15 data sets of pumps and SMBG of people with T1D (mean age 18.4 ± 4.8 years; eight females; mean glycated hemoglobin 8.2% ± 1.4% [66 ± 11mmol/mol]). The recommendations were compared among the physicians and between the physicians and the Advisor Pro. The study endpoint was the percentage of comparison points for which there was an agreement on the direction of insulin dose adjustments. Results: The percentage (mean ± SD) of agreement among the physicians on the direction of insulin pump dose adjustments was 51.8% ± 9.2%, 54.2% ± 6.4%, and 49.8% ± 11.6% for the basal, CR, and CF, respectively. The automated recommendations of the Advisor Pro on the direction of insulin dose adjustments were comparable )49.5% ± 6.4%, 55.3% ± 8.7%, and 47.6% ± 14.4% for the basal rate, CR, and CF, respectively( and noninferior to those provided by physicians. The mean absolute difference in magnitude of change between physicians was 17.1% ± 13.1%, 14.6% ± 8.4%, and 23.9% ± 18.6% for the basal, CR, and CF, respectively, and comparable to the Advisor Pro 11.7% ± 9.7%, 10.1% ± 4.5%, and 25.5% ± 19.5%, respectively, significant for basal and CR. Conclusions: Considerable differences in the recommendations for changes in insulin dosing were observed among physicians. Since automated recommendations by the Advisor Pro were similar to those given by physicians, it could be considered a useful tool to manage T1D.
An evaluation of the Hear Glue Ear mobile application for children aged 2–8 years old with otitis media with effusion
Authors: Fordington Surina , Brown Tamsin Holland 
Published: October 2020

Objectives To evaluate the acceptability and usability of the Hear Glue Ear mobile application to guide families and support speech and language development in children with otitis media with effusion (OME). To assess the validity of the app’s game-based hearing test to estimate changes in hearing levels between audiology appointments. Method This evaluation examined 60 children aged 2–8 with and without OME, attending Cambridge Community Audiology clinics. Children’s performance in the app’s hearing test was compared to their pure tone average (PTA) obtained in clinic. Children and caregivers completed questionnaires after their first interaction with the app, and after one week of using it at home. 18 clinicians completed anonymous questionnaires after trialling the app. Results Results from the app’s hearing test show a significant correlation with clinic PTA values ([Formula: see text]). 73.1% of caregivers supported their child using the app regularly and 85% thought it enabled them to give more accurate reports to clinicians. After one week, 87.0% of families downloaded and used the app at home, and 85.7% of these felt it provided strategies to help their child. 100% of children liked the app and 93.3% found it easy to use. 77.8% of clinicians supported patients using the app regularly. Conclusions Hear Glue Ear is acceptable to children, caregivers and clinicians as part of OME management. The app’s hearing test provides a valid estimate of fluctuating hearing levels. Hear Glue Ear is a free, accessible and family-centred intervention to provide trusted information and support development, as NICE guidance recommends.
Community Readiness for Drug Abuse Prevention in Two Rural Communities in Enugu State, Nigeria
Authors: Nwagu Evelyn N. , Dibia Samuel I. C. , Odo Amelia N. 
Published: October 2020

Background Drug abuse is an issue that affects all people regardless of gender, race, ethnicity, age, or socioeconomic status. It is associated with a range of physical, social, and economic consequences at both individual and community levels. Community involvement is necessary for effective drug abuse prevention. Communities need to be ready for such involvement. Methods We applied the Community Readiness Model and its assessment tool to determine the level of readiness for prevention of drug abuse in Obukpa and Ubollo communities of Nsukka and Udenu Local Government Areas of Enugu North Senatorial zone, Nigeria. Community Readiness Model and the Tool are very useful in identifying gaps in community readiness and capacity as well as guiding strategies for community initiatives. Twelve key informant interviews were conducted and analyzed according to procedures outlined in the Community Readiness handbook. Results The results of the study showed that members of Obukpa and Ubollo communities were at the vague awareness stage. SWOT analysis of the qualitative data revealed the strengths, weaknesses, opportunities and threats within the communities for an intended intervention to increase level of readiness. One such strength is the church, which was identified as a viable means of disseminating information on drug abuse prevention in both communities. Conclusion Rural communities are vulnerable to drug-related problems as a result of low levels of awareness of the dangers of drug abuse. The findings of this study provided strategies and directions for future intervention programmes in the study area. Nurses and other health personnel involved in Community Health Education should take cognizance of the strengths, weaknesses and opportunities within the communities in designing drug abuse prevention intervention programmes.
SARS-CoV-2–specific T cells are rapidly expanded for therapeutic use and target conserved regions of the membrane protein
Authors: Keller Michael D. , Harris Katherine M. , Jensen-Wachspress Mariah A. , Kankate Vaishnavi V. , Lang Haili , Lazarski Christopher A. , Durkee-Shock Jessica , Lee Ping-Hsien , Chaudhry Kajal , Webber Kathleen , Datar Anushree , Terpilowski Madeline , Reynolds Emily K. , Stevenson Eva M. , Val Stephanie , Shancer Zoe , Zhang Nan , Ulrey Robert , Ekanem Uduak , Stanojevic Maja , Geiger Ashley , Liang Hua , Hoq Fahmida , Abraham Allistair A. , Hanley Patrick J. , Cruz C. Russell , Ferrer Kathleen , Dropulic Lesia , Gangler Krista , Burbelo Peter D. , Jones R. Brad , Cohen Jeffrey I. , Bollard Catherine M. 
Published: October 2020

AbstractT-cell responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been described in recovered patients, and may be important for immunity following infection and vaccination as well as for the development of an adoptive immunotherapy for the treatment of immunocompromised individuals. In this report, we demonstrate that SARS-CoV-2–specific T cells can be expanded from convalescent donors and recognize immunodominant viral epitopes in conserved regions of membrane, spike, and nucleocapsid. Following in vitro expansion using a good manufacturing practice-compliant methodology (designed to allow the rapid translation of this novel SARS-CoV-2 T-cell therapy to the clinic), membrane, spike, and nucleocapsid peptides elicited interferon-γ production, in 27 (59%), 12 (26%), and 10 (22%) convalescent donors (respectively), as well as in 2 of 15 unexposed controls. We identified multiple polyfunctional CD4-restricted T-cell epitopes within a highly conserved region of membrane protein, which induced polyfunctional T-cell responses, which may be critical for the development of effective vaccine and T-cell therapies. Hence, our study shows that SARS-CoV-2 directed T-cell immunotherapy targeting structural proteins, most importantly membrane protein, should be feasible for the prevention or early treatment of SARS-CoV-2 infection in immunocompromised patients with blood disorders or after bone marrow transplantation to achieve antiviral control while mitigating uncontrolled inflammation.
2-D chemical structure image-based in silico model to predict agonist activity for androgen receptor
Authors: Yu Myeong-Sang , Lee Jingyu , Lee Yongmin , Na Dokyun 
Published: October 2020

Abstract
Background
Abnormal activation of human nuclear hormone receptors disrupts endocrine systems and thereby affects human health. There have been machine learning-based models to predict androgen receptor agonist activity. However, the models were constructed based on limited numerical features such as molecular descriptors and fingerprints.

Result
In this study, instead of the numerical features, 2-D chemical structure images of compounds were used to build an androgen receptor toxicity prediction model. The images may provide unknown features that were not represented by conventional numerical features. As a result, the new strategy resulted in a construction of highly accurate prediction model: Mathews correlation coefficient (MCC) of 0.688, positive predictive value (PPV) of 0.933, sensitivity of 0.519, specificity of 0.998, and overall accuracy of 0.981 in 10-fold cross-validation. Validation on a test dataset showed MCC of 0.370, sensitivity of 0.211, specificity of 0.991, PPV of 0.882, and overall accuracy of 0.801. Our chemical image-based prediction model outperforms conventional models based on numerical features.

Conclusion
Our constructed prediction model successfully classified molecular images into androgen receptor agonists or inactive compounds. The result indicates that 2-D molecular mimetic diagram would be used as another feature to construct molecular activity prediction models.
How does ageism influence frailty? A preliminary study using a structural equation model
Authors: Ye Bo , Gao Junling , Fu Hua , Chen Hao , Dong Wenjing , Gu Ming 
Published: October 2020

AbstractBackgroundBased on the Stereotype Embodiment Theory (SET), this study aims to examine the mechanism of ageism on frailty through the proposed framework of “Experiences of Ageism (EA) → Age Stereotypes (AS) → Attitudes to Ageing (AA) → Frailty” using a structural equation model (SEM).MethodsA community-based study involving 630 participants aged 60 years and older was conducted in Shanghai. EA, AS, AA and frailty status were assessed by validated scales. In particular, EA included three parts in this study, as the first part was the experiences of explicit prejudice or discrimination because of age, another two parts were the experiences of witnessed and encountered implicit negative age-based stereotypes. A SEM was performed to examine whether the proposed paths from EA to frailty were supported.ResultsEA had a significant indirect effect (β’ = .360*-.456*-.576 = .095,p < .001) on frailty through the path of “EA → AS → AA → Frailty” after controlling for covariates. AA had a direct effect (β = −.576,p < .001) on frailty; AS fully mediated the association between EA and AA (indirect effect = .360*-.456 = −.164,p < .001), and AA fully mediated the association between AS and frailty (indirect effect = −.456*-.576 = .263,p < .001).ConclusionsThese findings demonstrated a mechanism from ageism to frailty, and highlighted the potential threat of negative AS on health. Ageism and frailty are both great challenges for the process of healthy ageing.
Genomic evidence for recurrent genetic admixture during the domestication of Mediterranean olive trees (Olea europaea L.)
Authors: Julca Irene , Marcet-Houben Marina , Cruz Fernando , Gómez-Garrido Jèssica , Gaut Brandon S. , Díez Concepción M. , Gut Ivo G. , Alioto Tyler S. , Vargas Pablo , Gabaldón Toni 
Published: October 2020

Abstract
Background
Olive tree (Olea europaea L. subsp. europaea, Oleaceae) has been the most emblematic perennial crop for Mediterranean countries since its domestication around 6000 years ago in the Levant. Two taxonomic varieties are currently recognized: cultivated (var. europaea) and wild (var. sylvestris) trees. However, it remains unclear whether olive cultivars derive from a single initial domestication event followed by secondary diversification, or whether cultivated lineages are the result of more than a single, independent primary domestication event. To shed light into the recent evolution and domestication of the olive tree, here we analyze a group of newly sequenced and available genomes using a phylogenomics and population genomics framework.

Results
We improved the assembly and annotation of the reference genome, newly sequenced the genomes of twelve individuals: ten var. europaea, one var. sylvestris, and one outgroup taxon (subsp. cuspidata)—and assembled a dataset comprising whole genome data from 46 var. europaea and 10 var. sylvestris. Phylogenomic and population structure analyses support a continuous process of olive tree domestication, involving a major domestication event, followed by recurrent independent genetic admixture events with wild populations across the Mediterranean Basin. Cultivated olives exhibit only slightly lower levels of genetic diversity than wild forms, which can be partially explained by the occurrence of a mild population bottleneck 3000–14,000 years ago during the primary domestication period, followed by recurrent introgression from wild populations. Genes associated with stress response and developmental processes were positively selected in cultivars, but we did not find evidence that genes involved in fruit size or oil content were under positive selection. This suggests that complex selective processes other than directional selection of a few genes are in place.

Conclusions
Altogether, our results suggest that a primary domestication area in the eastern Mediterranean basin was followed by numerous secondary events across most countries of southern Europe and northern Africa, often involving genetic admixture with genetically rich wild populations, particularly from the western Mediterranean Basin.
Communities on the move: community participation in health in rural territories of Buenaventura District in Colombia
Authors: Blandón-Lotero Laura Catalina , Jaramillo-Mejía Marta Cecilia 
Published: October 2020

AbstractBackgroundSocial and community participation is a fundamental component of the development of renewed primary healthcare (PHC). With the recognition of health as a right, such participation is a significant part of the design of public policies aimed at this sector. These policies contribute not only to overcoming inequity in the provision of this type of services but also to a reduction in social inequalities as a whole. Through a comparative analysis, this study aimed to explain the conditions through which ethnic-rural territories of the Colombian Pacific coast participate in health to contribute to the generation of policies and programs in territories with similar conditions.MethodsThe work was developed through the use of multiple techniques and strategies for information collection and analysis. These include several semi-structured interviews, multiple observation exercises and analysis based on a set theory, i.e., qualitative comparative analysis (QCA). The latter aims to develop a model that provides a count of the main causal combinations that allow high community participation in health.ResultsKey findings include how the trajectory of social mobilization and existence of a robust community social fabric became two critical conditions for community participation in the context of social exclusion. The presence of variables such as the implementation of PHC, guarantee of social rights, and trust in institutions, is underestimated as sufficient causal conditions for obtaining this result. Therefore, it is essential to recognize the existence, validity, and importance of processes, experiences, and resourcefulness of political natures, which aim at transforming the daily reality of the inhabitants of these communities. These also set a potential space and scenario for managing the communities’ main problems, including health, in the absence of institutionality that guarantees access to their social rights.ConclusionThis study points out the importance of understanding community participation as a political activity, expanding exchange dynamics and dialogs between institutions, rulers, and communities to provide social responses in health and well-being to communities and to understand local realities and their own community dynamics.