bluuebunny/crossref_metadata_2025_split huggingface dataset:
MetaCNV - a consensus approach to infer accurate copy numbers from low coverage data
Authors: Friedrich Stefanie , Barbulescu Remus , Helleday Thomas , Sonnhammer Erik LL 
Published: February 2020

Abstract
Background : The majority of copy number callers requires high read coverage data that is often achieved with elevated material input, which increases the heterogeneity of tissue samples. However, to gain insights into smaller areas within a tissue sample, e.g a cancerous area in a heterogeneous tissue sample, less material is used for sequencing, which results in lower read coverage. Therefore, more focus needs to be put on copy number calling that is sensitive enough for low coverage data. Results : We present MetaCNV, a copy number caller that infers reliable copy numbers for human genomes with a consensus approach. MetaCNV specializes in low coverage data, but also performs well on normal and high coverage data. MetaCNV integrates the results of multiple copy number callers and infers absolute and unbiased copy numbers for the entire genome. MetaCNV is based on a meta-model that bypasses the weaknesses of current calling models while combining the strengths of existing approaches. Here we apply MetaCNV based on ReadDepth, SVDetect, and CNVnator to real and simulated datasets in order to demonstrate how the approach improves copy number calling. Conclusions : MetaCNV, available at https://bitbucket.org/sonnhammergroup/metacnv , provides accurate copy number prediction on low coverage data and performs well on high coverage data.
“The Hardest Job You Will Ever Love”: Nurse Recruitment, Retention, and Turnover in the Nurse-Family Partnership Program in British Columbia, Canada
Authors: Campbell Karen A , Borek Natasha Van , Marcellus Lenora , Landy Christine Kurtz , Jack Susan M 
Published: February 2020

AbstractBackground: Nurse turnover is a significant issue and complex challenge for all sectors of healthcare and is exacerbated by a global nursing shortage. Nurse-Family Partnership is a community health program for first-time pregnant and parenting girls and young women living in situations of social and economic disadvantage, delivered exclusively by public health nurses and only within a research context in Canada. The aim of this article is to explore and describe factors that contribute to recruitment, retention, and turnover of public health nurses delivering Nurse-Family Partnership in British Columbia, Canada. Methods: Interpretive description was used to guide sampling, data collection and analytic decisions in this qualitative component drawn from the British Columbia Healthy Connections Project mixed methods process evaluation. Semi-structured, individual interviews were conducted with 28 public health nurses who practiced in and then exited Nurse-Family Partnership. Results: Nurses were motivated to join this program because they wanted to deliver an evidence-based program for vulnerable young mothers that fit with their personal and professional philosophies and offered nurse autonomy. Access to program resources attracted nursing staff, while delivering a program that prioritizes maintaining relationships and emphasizes client successes was a positive work experience. Opportunities for ongoing professional development/ education, strong team connections, and working at full-scope of nursing practice were significant reasons for nurses to remain in Nurse-Family Partnership. Personal circumstances (retirement, family/health needs, relocation, career advancement) were the most frequently cited reasons leading to turnover. Other factors included: involuntary reasons, organizational and program factors, and geographical factors. Conclusions: Public health organizations that deliver Nurse-Family Partnership may find aspects of job embeddedness theory useful for developing strategies for supporting recruitment and retention and reducing nurse turnover. Hiring nurses who are the right fit for this type of program may be a useful approach to increasing nurse retention. Fostering a culture of connectivity through team development along with supportive and communicative supervision are important factors associated with retention and may decrease turnover. Many involuntary factors were specific to being in a study environment. Program, organizational, and geographical factors affecting nurse turnover are modifiable.
Feasibility of combining two individualized lung recruitment maneuvers at birth for very low gestational age infants: a retrospective cohort study
Authors: Kanaan Zalfa , Bloch-Queyrat Coralie , Boubaya Marouane , Lévy Vincent , Bolot Pascal , Waszak Paul 
Published: February 2020

Abstract
BACKGROUND Lung recruitment at birth has been advocated as an effective method of improving the respiratory transition at birth. Sustained inflations (SI) and dynamic positive end-expiratory pressure (PEEP) were assessed in clinical and animal studies to define the optimal level. Our working hypothesis was that very low gestational age infants (VLGAI) < 32 weeks’ gestation require an individualized lung recruitment based on combining both manoeuvers. METHODS Between 2014 and 2016, 91 and 72 inborn VLGAI, requiring a respiratory support beyond a continuous positive airway pressure (CPAP) = 5 cmH2O, were enrolled before and after introducing these manoeuvers based on progressive increase in SI up to 15 seconds, with simultaneous gradual increase in PEEP up to 15 cmH2O, according to the cardiorespiratory response. Retrospective comparisons of the rate of mechanical ventilation (MV) < 72h of life, short-term and before discharge morbidity were then performed. RESULTS Among extremely low gestational age infants (ELGAI) < 29 weeks’ gestation, the following outcomes decreased significantly: intubation (90% to 55%) and surfactant administration (54% to 12%) in the delivery room, MV (92% to 71%) and its mean duration < 72h of life (45h to 13h), administration of a 2nd dose of surfactant (35% to 12%) and postnatal corticosteroids (52% to 19%), and the rate of bronchopulmonary dysplasia (23 to 5%). Among VLGAI, all of these results were also significant. Neonatal mortality and morbidity were not different. CONCLUSIONS In our setting, combining two individualized lung recruitment maneuvers at birth was feasible and may be beneficial on short-term and before discharge pulmonary outcomes. A randomized controlled trial is needed to confirm these results.
Prognostic potential of pre-partum blood biochemical and immune variables for postpartum mastitis risk in dairy cows
Authors: Guan Ruowei , Wang Diming , Wang Beibei , Jiang Luyi , Liu Jianxin 
Published: February 2020

Abstract
Background: Mastitis is the most frequent diseases for transition cows. Identification of potential biomarkers for diagnosis of mastitis is important for its prevention. Thus, this study was conducted to investigate blood variables related to lipid metabolism, oxidative stress and inflammation, and serum variables that are related to health in postpartum cows. Results: Seventy-six healthy Holstein dairy cows at week 4 before calving were selected to collect blood samples from weeks -4 to 4 weekly relative to calving, respectively. Milk yield and composition were recorded weekly. According to the cut-off of somatic cell counts (SCC) for diagnosis of mastitis, 33 cows with SCC ≥ 500,000 cells ml-1, 20 cows with 200,000 cells ≤ SCC < 500,000 cells ml-1, and 23 cows with SCC < 200,000 cells ml-1 were defined as high, middle, and low SCC, respectively. Serum concentrations of β-hydroxybutyrate were higher (P < 0.01) during all weeks, and non-esterified fatty acids were higher in high SCC than in low SCC cows from weeks -3 to 2 relative to calving. Higher serum concentrations of superoxide dismutase (P < 0.01) and lower malondialdehyde levels (P < 0.01) in low SCC than in high SCC cows indicate that the latter suffered from oxidative stress. The difference analysis of the three groups suggested that none of the above-mentioned variables can be used as potential prognostic candidates. On the other hand, high SCC cows exhibited higher blood neutrophil to lymphocyte ratio (NLR, P < 0.01) and platelet to lymphocyte ratio (PLR, P < 0.01) than low SCC cows, with a higher NLR (P < 0.01) in middle SCC than in low SCC cows. The high SCC cows had lower levels of anti-inflammatory factors including IL-10 (P = 0.05), but higher levels of proinflammatory factors such as IL-6 (P < 0.01), TNF-α (P < 0.05), and PSGL-1 (P < 0.01) than low SCC cows.Conclusions: The significantly different NLR and PLR pre-partum between the middle and low SCC cows suggest their prognostic potential for postpartum mastitis risk.
Hcfc1a regulates neural precursor proliferation and asxl1 expression in the developing brain.
Authors: Castro Victoria L. , Reyes Joel F. , Reyes-Nava Nayeli , Paz David , Quintana Anita M 
Published: February 2020

Abstract
Background: Precise regulation of neural precursor cell (NPC) proliferation and differentiation is essential to ensure proper brain development and function. The HCFC1 gene encodes a transcriptional co-factor that regulates cell proliferation, and previous studies suggest that HCFC1 regulates NPC function. However, the molecular mechanism underlying these cellular deficits has not been completely characterized. Methods: Here we created a zebrafish harboring mutations in the hcfc1a gene (the hcfc1a co60/+ allele), one ortholog of HCFC1 and utilized immunohistochemistry and RNA-sequencing technology to understand the function of hcfc1a during neural development. Results: The hcfc1a co60/+ allele results in an increased number of NPCs, neurons, and radial glial cells. These deficits are associated with the abnormal expression of asxl1 , a polycomb transcription factor, which we identified as a downstream effector of hcfc1a using high throughput RNA sequencing technology. Inhibition of asxl1 activity and/or expression in larvae harboring the hcfc1a co60/+ allele completely restored the number of NPCs to normal levels. Conclusion: Collectively, our data demonstrate a novel pathway in which hcfc1a regulates NPCs and neurogenesis.
Operative versus nonoperative treatment in children with painful rigid flatfoot and talocalcaneal coalition. Results from a retrospective comparative study.
Authors: Gennaro Giovanni Luigi Di , Stallone Stefano , Olivotto Eleonora , Zarantonello Paola , Magnani Marina , Tavernini Tullia , Stilli Stefano , Trisolino giovanni 
Published: February 2020

Abstract
Background: The management of painful rigid flatfoot (RFF) with talocalcaneal coalition (TCC) is controversial. We aimed to compare operative and nonoperative treatment in children with RFF and TCC.
Methods: We retrospectively reviewed medical records and radiographs of children with RFF and TTC treated between 2005 and 2015. The nonoperative treatment consisted of manipulation under anesthesia, cast immobilization and shoe insert after cast removal. The operative treatment consisted of combined TCC resection, graft interposition and subtalar arthroereisis.
Results: Thirty-four Children (47 feet) in the nonoperative group and twenty-one children (34 feet) in the operative group were included. No differences were found between groups, concerning baseline characteristics. The mean age at treatment was 11.8 years (9-17): 11.6 (9-17) for the nonoperative group, 12.2 (10-15) for the operative group. The mean follow-up averaged 6.6 (3-12) years and was significantly longer in the nonoperative group (7.8 versus 4.7 years; p < 0.0005), since the operative procedure was increasingly practiced in the latest years.
There were no complications in either groups, but 6 patients (7 feet) in the nonoperative group were unsatisfied and required surgery. At the latest follow-up, the AOFAS-AHS improved in both groups, although the operative group showed significantly better improvement. The operative group reported also significantly better FADI score, after adjustment for follow-up and baseline variables.
Conclusions: The operative treatment showed better results compared to the nonoperative treatment. Symptomatic RFF with TCC in children can be effectively treated in one step with resection, graft interposition and subtalar arthroereisis. Further prospective randomized studies are needed to confirm our findings and to identify the best operative strategy in this condition.
Validation of the Spanish version of Migraine Disability Assessment questionnaire (MIDAS) in migraine university students.
Authors: Rodríguez-Almagro Daniel , Achalandabaso Alexander , Rus Alma , Obrero-Gaitán Esteban , Zagalaz-Anula Noelia , Lomas-Vega Rafael 
Published: February 2020

Abstract
Background. The Migraine Disability Assessment (MIDAS) questionnaire is widely used to determine the degree of migraine-related disability of subjects. So far, and to the best of our knowledge, no Spanish version of this tool has been validated. The questionnaire comprises seven items, with the first five constituting the main scale while the sixth and seventh items referring, respectively, to the frequency and intensity of headache. The present study aims to analyze the clinimetric properties of the Spanish version of the MIDAS questionnaire in a population of university students. Methods. We performed a cross-sectional study of validation for this measuring instrument. A total of 153 subjects participated in the study. We analyzed construct validity using factor analysis, test-retest reliability by the Intraclass Correlation Coeficient (ICC), internal consistency, and concurrent validity with respect to the 12-Item Short Form Health Survey (SF-12). Results. Factor analysis revealed a two-factor structure. The questionnaire has good reliability for the MIDAS main-scale score ([ICC=0.81; 95% CI: 0.63 – 0.90]), excellent reliability for headache frequency (ICC=0.90; 95%; CI: [0.79 – 0.95]), and moderately good reliability for headache intensity (ICC=0.63; 95% CI: [0.34 – 0.80]). The analysis also yielded good internal consistency results (α Cronbach=0.797) and a moderate correlation between MIDAS-main scale and the physical summary component of SF-12 (Rho=-0.326; p < 0.001). Conclusions. The Spanish version of the MIDAS questionnaire is a valid and reliable tool to measure migraine-related disability in university subjects. The two additional items provide information that could help clinicians in making decisions.
Bone transport versus acute shortening for the management of infected tibial bone defects: a meta-analysis
Authors: Wen Hongjie , Zhu Shouyan , Li Canzhang , Xu Yongqing 
Published: February 2020

Abstract
Background: The treatment for infected tibial bone defects can be a great challenge for the orthopaedic surgeon. This meta-analysis was conducted to compare the safety and efficacy between bone transport (BT) and the acute shortening technique (AST) in the treatment of infected tibial bone defects. Methods: A literature survey was conducted by searching the PubMed, Web of Science, Cochrane Library, and Embase databases together with the China National Knowledge Infrastructure (CNKI) and the Wanfang database for articles published up to 9 August 2019. The modified Newcastle-Ottawa scale (NOS) was adapted to evaluate the bias and risks in each eligible study. The data of the external fixation index (EFI), bone grafting, bone and functional results, complications, bone union time and characteristics of participants were extracted. RevMan v.5.3 was used to perform relevant statistical analyses. Standard mean difference (SMD) was used for continuous variables and relative risk (RR) for the binary variables. All of the variables included its 95% confidence interval (CI). Results: Five studies, including a total of 199 patients, were included in the study. Statistical significance was observed in the EFI (SMD = 0.63, 95% CI: 0.25, 1.01, P = 0.001) and bone grafting (RR = 0.26, 95%CI: 0.15, 0.46, P <0.00001); however, no significance was observed in bone union time (SMD = –0.02, 95% CI: –0.39, 0.35, P = 0.92), bone results (RR = 0.97, 95% CI: 0.91, 1.04, P = 0.41), functional results (RR = 0.96, 95% CI: 0.86, 1.08, P = 0.50) and complications (RR = 0.76, 95% CI: 0.41, 1.39, P = 0.37). Conclusions: AST is preferred from the aspect of minimising the treatment period, whereas BT is superior to AST for reducing bone grafting. Due to the limited number of trials, the meaning of this conclusion should be taken with caution for infected tibial bone defects.
Construction and analysis of an artificial consortium based on fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce platform chemical 3-hydroxypropionic acid from CO2
Authors: Zhang Li , Chen Lei , Diao Jinjin , Song Xinyu , Shi Mengliang , Zhang Weiwen 
Published: February 2020

Abstract
Background: Cyanobacterial carbohydrates, such as sucrose, have been considered as a potential renewable feedstock to support the production of fuels and chemicals. However, the separation and purification processes of these carbohydrates will increase the production cost of chemicals. It has been proposed that co-culture fermentation is an efficient and economical way to utilize these cyanobacterial carbohydrates. However, studies about the application of co-culture system to achieve the green biosynthesis of platform chemicals are still rare.Results: In this study, we successfully achieved one-step conversion of sucrose derived from cyanobacteria to fine chemicals by constructing a microbial consortium consisted of fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 and Escherichia coli to sequentially produce sucrose and then platform chemical 3-hydroxypropionic acid (3-HP) from CO2 under photoautotrophic growth condition. First, efforts were made to overexpress sucrose permease coding gene cscB under strong promoter Pcpc560 in S. elongatus UTEX 2973 for efficient sucrose secretion. Second, sucrose catabolic pathway and malonyl CoA-dependent 3-HP biosynthetic pathway were introduced into E. coli BL21(DE3) for heterologous biosynthesis of 3-HP from sucrose. By optimizing the cultivation temperature from 37℃ to 30℃, a stable artificial consortium system was constructed with the capability of producing 3-HP up to 68.29 mg/L directly from CO2. In addition, cell growth of S. elongatus UTEX 2973 in the consortium was enhanced, probably due to the quick quenching of reactive oxygen species (ROS) in the system by E. coli, which in turn improved photosynthesis of cyanobacteria. Conclusion: The study demonstrated the feasibility of achieving the one-step conversion of sucrose to fine chemicals using an artificial consortium system. The study also confirmed that the heterotrophic bacteria could promote cell growth of cyanobacteria by relieving oxidative stress in this microbial consortium, which further suggests the potential values of this system for the future industrial application.
Influence of talent management practices on service delivery of doctors in Nairobi, Kenya: A descriptive research study
Authors: Onuko Christine , Onyango Joseph 
Published: February 2020

Abstract
Background : A reliable and dedicated health workforce is instrumental in the attainment of Universal Health Coverage and Sustainable Development Goal (SDG) three on health. This is even more appropriate for Kenya where there is a dire shortage of health workers, and where health workforce management has been devolved to county governments. This study focusses on the influence of talent management practices (attraction, training and development, and retention) on doctor’s service delivery in four county hospitals in Nairobi, Kenya. Method : The study through a descriptive research design used quantitative questionnaires to gather data from 108 doctor respondents from four County Hospitals (Mbagathi, Mama Lucy Kibaki, Pumwani Maternity, and Mutuini Hospitals) in Nairobi, Kenya. Inferential statistics were used to analyse the data. Results : Findings show that there is a positive correlation between attraction, training and development and service delivery of doctors. Employee retention, however, did not have an association with service delivery. Conclusions : As Kenya adjusts to a devolved system of governance in relation to health, it is important that the attraction, training and development, as well as retention of doctors is given serious consideration if service delivery is to be improved.
Comparison of the Level of Familiarity of IT Units' Staff and students with Big Data Analyzes
Authors: Nazari Elham , Asgari Parnian , tabesh hamed 
Published: February 2020

Abstract
introduction The rapid development of technology in recent decades has led to the production of a huge amount of data. This type of data analysis that is called Big Data Analysis obtain Many benefits, including reducing costs. One of the challenges of these analyses is the lack of specialized expertise and knowledge in this area. The purpose of this study was to compare the familiarity of IT staff and students with big data analyzes at various universities and organizations. Materials and method This analytical study was conducted on IT units' staff and students of different organizations and universities in Mashhad, Iran. A questionnaire was designed based on reviewing the texts published in PubMed, google scholar, science direct, and EMBASE databases and using the Delphi method and the attendance of 10 specialists in different disciplines. The designed questionnaire evaluated the participants' knowledge about the Big Data analyzes in two parts. The participants were 265 IT units' staff and students of different organizations, completing the designed questionnaire. Participants' opinion was evaluated using two descriptive and analytical approaches. The relationship between knowledge scores and individual characteristics such as gender, age, work experience, Field of study, degree, the average number of hours’ scientific study and non-scientific study per week was examined. To investigate the synchronous and reciprocal effects GLM was used. Results Scores earned by students and staff were 2.66 ± 1.13 and 2.28 ± 1.21 respectively that p =. 012 represented a significant correlation between the level of knowledge of students and staff. In other words, the level of knowledge of staff about big data was more than the level of knowledge of the students.The correlation of each of the variables was not significant with the score of the Big Data Analysis Knowledge.But There was a significant correlation between experience and gender with the knowledge scores. Conclusions In general, the level of knowledge in analyzing big data in different groups of people was at a low level that implementing measures such as holding training courses in this field seems necessary.