bluuebunny/crossref_metadata_2025_split huggingface dataset:
The loss of DLG2 isoform 7/8, but not isoform 2, is critical in advanced staged neuroblastoma
Authors: Keane Simon , Martinsson Tommy , Kogner Per , Ejeskar Katarina 
Published: October 2020

Abstract
BACKGROUNDNeuroblastoma is a childhood neural crest tumor showing large clinical and genetic heterogeneity, one form displaying 11q-deletion is very aggressive. It has been shown that 11q-deletion results in decreased expression of DLG2, a gene residing in the deleted region. DLG2 has a number of different isoforms with the main difference is the presence or absence of a L27 domain. The L27 domain containing DLG proteins can form complexes with CASK/MPP and LIN7 protein family members, which will control cell polarity and signaling. METHODSWe evaluated the DLG gene family and the LIN7 gene family for their expression in differently INSS staged neuroblastoma from publically available neuroblastoma data and primary tumors, we included two distinct DLG1 and DLG2 N-terminal transcript isoforms encoding L27 domains for their expression. Functionality of DLG2 isoforms and of LIN7A were evaluated in the 11q-deleted neuroblastoma cell line SKNAS.RESULTSIn neuroblastoma only two DLG2 isoforms were expressed: isoform 2 and isoform 7/8. Using the array data we could determine that higher expression of DLG members that contain L27 domains correlated to better survival and prognosis. Whilst DLG1 showed a decrease in both isoforms with increased INSS stage, only the full length L27 containing DLG2 transcripts DLG2-isoform 7/8 showed a decrease in expression in high stage neuroblastoma. We could show that the protein encoded by DLG2-isoform 7 could bind to LIN7A, and increased DLG2-isoform 7 gene expression increased the expression of LIN7A, this reduced neuroblastoma cell proliferation and viability.CONCLUSIONWe have provided evidence that gene expression of the L27 domain containing DLG2-isoform 7/8 but not L27 domain lacking DLG2-isoform 2 is disrupted in neuroblastoma, in particular in the aggressive subsets of tumors. The presence of the complete L27 domain allows for the binding to LIN7A, which will control cell polarity and signaling, thus affecting cancer cell viability.
Associations of Total Serum IgE and Peripheral Blood Eosinophils with Childhood Asthma: a Longitudinal Retrospective Study
Authors: LEE JYH-HONG , Chu Ai-Lin , Hsiao Shu-Ya , Chang Kuei-Ying , Yang Yao-Hsu , Lin Yu-Tsan , Wang Li-Chieh , Yu Hsin-Hui , Hu Ya-Chiao , Chiang Bor-Luen 
Published: October 2020

Abstract
BackgroundChildhood asthma is a heterogeneous disease. The relationship of total serum IgE and peripheral blood eosinophil with childhood asthma are controversial. To determine whether total serum IgE and peripheral blood eosinophil are associated with long-term lung function, asthma severity and asthma control in children.MethodsWe conducted a longitudinal retrospective study in 383 childhood asthma patients with total serum IgE and peripheral blood eosinophil obtained at baseline. Peak expiratory flow (PEF) variability, predicted PEF percentage, asthma severity, asthma control test (ACT) score, and asthma control from each follow-up visit were recorded as phenotype outcomes. We correlated serum IgE and blood eosinophil with phenotype outcomes using generalized estimating equations. Additionally, we compared expression of microRNA in peripheral blood cells from nonallergic versus allergic patients.ResultsTotal serum IgE was positively associated with asthma severity (OR = 1.0014, P = 0.0071). Peripheral blood eosinophil was positively related to PEF variability (OR = 1.0052, P = 0.0002). Association of inhaled corticosteroids (ICS) dose with phenotype outcomes were not significant. Compared with allergic patients, children with nonallergic asthma had higher asthma severity. We identified six interaction pathways specific to IgE from eight top differentially expressed microRNAs. ConclusionsBeside asthma control, total serum IgE is predictive of long-term asthma severity and peripheral blood eosinophil is closely related to PEF variability. We suggest that total serum IgE might be involved in modulating disease severity of both allergic and nonallergic asthma while peripheral blood eosinophilia reflects the status of longitudinal lung function.
Reply to “Comment on Geodesic Cycle Length Distributions in Delusional and Other Social Networks”
Authors: Stivala Alex 
Published: October 2020

Abstract
Martin (2020) describes a misinterpretation of exponential random graph (ERGM) parameters in my contribution (Stivala 2020), with the use of this parametric model obscuring, rather than illuminating, the data. He suggests that this is symptomatic of a trend in the social networks community towards a methodological monoculture focussed on the use of ERGMs. In this Reply I try to clarify how this situation arose in this specific case, and address some more general issues Martin raises, including the use of nodal covariates, what we can learn from ERGMs, and methodological monoculturalism in social network research.
The Unobserved Economy – Invisible Production in Households. The Household Production Satellite Account and the National Time Transfer Account
Authors: Marszałek Marta 
Published: October 2020

Abstract
Standard measures of economic activity relate to goods and services offered by the market. Stiglitz’s report, however, suggests that not only monetary value or economic products create welfare, but non-monetary components should also be included in the System of National Accounts. Although household production is registered in official statistics, the main part of it, i.e. nearly 75-80% of the total home production remains outside of the GDP. The Household Production Satellite Account (HHSA) is a macroeconomic analysis covering both market and non-market home production. The National Time Transfer Accounts (NTTA) is, next to HHSA, an analysis aimed to register and observe the directions of transfers and to present the recipients and givers of home production. Regular estimations provided by the HHSA and NTTA may prove a valuable supporting tool to national accounts, pension systems, or social policy as they provide a great deal of macroeconomic information regarding households, their economic and living conditions, social changes, and welfare.
Ferropyrosmalite-bearing fluid inclusions in the North Patagonian Andes metasedimentary basement, Argentina: A record of regional metasomatism
Authors: Sosa Graciela , Oriolo Sebastián , van den Kerkhof Alfons , González Pablo Diego , Olaizola Ezequiel , Bechis Florencia 
Published: October 2020

AbstractQuartz segregations in paragneisses from the Paleozoic basement of the North Patagonian Andes contain highly saline multiphase fluid inclusions with the rare daughter mineral ferropyrosmalite detected by Raman analysis, besides halite, sylvite, hematite, and/or magnetite. During heating experiments, L-V homogenization occurs (256–515 °C), followed by halite dissolution (287–556 °C) and the dissolution of ferropyrosmalite at 550–581 °C. The latter phase transition triggers the growth of clinoamphibole crystals according to the following idealized reactions, written for potential end-members:4 F e 8 S i 6 O 15 [ ( O H ) 6 C l 4 ] + 6 C a 2 + ( a q ) Ferropyrosmalite ↔ 3 C a 2 F e 5 S i 8 ↔ O 22 ( O H ) 2 + 17 F e 2 + ( a q ) + 16 C l − ( a q ) + 12 O H − + 3 H 2 Ferro-actinolite F e 8 S i 6 O 15 [ ( O H ) 6 C l 4 ] + 2 C a 2 + ( a q ) Ferropyrosmalite + Fe 3 + ( aq ) + 2Al 3 + ( aq ) + Na + ( aq ) + H 2 O ↔ Na C a 2 ( Fe 4 2 + F e 3 + ) ( Al 2 Si 6 ) ↔ O 22 Cl 2 + 4 F e 2 + ( a q ) + 2 C l − ( a q ) + 4 H 2 Chloro-hastingsiteFerropyrosmalite ↔ Chloro-hastingsite The amphibole resembles the composition of ferro-actinolite but also has striking similarities with chloro-hastingsite, as indicated by Raman spectroscopy. During the heating experiment, hematite (when present) transforms to magnetite by the uptake of H2, whereas inclusions without Fe-oxides contain traces of H2 after the reaction. This mineral transformation shows that ferropyrosmalite might result from the retrograde re-equilibration of amphibole with the brine, implying the uptake of Fe2+, Cl–, and H2O and the enrichment of Ca2+ in the brine. Pervasive fluid flow and fluid-assisted diffusion are recorded by channel way microstructures, healed microfractures, and dissolution-reprecipitation phenomena, as demonstrated by cathodoluminescence microscopy. These alkali- and FeCl2-rich brines, derived from magmatic sources and of possible Mesozoic age, were related to regional metasomatism, coeval with widespread granitoid activity.
Respon Mahasiswa terhadap Pembelajaran Interaktif dengan Animasi 3D melalui Powerpoint
Authors: Marpaung Dewi Natalia , Pongkendek Jesi Jecsen 
Published: October 2020

<p class="E-JournalAbstractBodyEnglish">Pembelajaran harus di dukung oleh dosen yang kreatif untuk menciptakan media pembelajaran interaktif agar mahasiswa lebih aktif, kreatif dan inovatif selama proses pembelajaran berlangsung. Tujuan dari penelitian ini adalah untuk mengetahui respon mahasiswa terhadap mediapembelajaran interaktif yang digunakan oleh dosen dengan menerapkan animasi 3D melalui <em>Powerpoint</em>, penelitian ini adalah penelitian deskriptif, penelitian ini dilakukan di universitas musamus jurusan pendidikan kimia. Sampel dalam penelitian ini terdiri dari 20 orang mahasiswa. Pengumpulan data dilakukan melalui <em>google form</em> yang dapat diakses oleh masing-masing mahasiswa. Berdasarkan hasil penelitian yang dilakukan mahasiswa memberikan respon yang positif terhadap animasi 3D yang digunakan oleh dosen dengan nilai 4,5 dari 5.0 dan dikategorikan sangat baik. Sehingga media pembelajaran interaktif dengan menggunakan animasi 3D melalui powerpoint dapat digunakan untuk menjadikan mahasiswa termotivasi untuk belajar di dalam kelas dan membantu pemahaman mahasiswa terhadap materi yang diajarkan oleh dosen.</p>
Degradation of hydroxypropyl guar gum at wide pH range by a heterogeneous Fenton-like process using bentonite-supported Cu(0)
Authors: Zhou Ling , Xu Zhongying , Zhang Jie , Zhang Zhifang , Tang Ying 
Published: October 2020

AbstractTo seek for efficient Fenton-like oxidation processing for treatment of waste fracturing fluid containing hydroxypropyl guar gum (HPGG), in heterogeneous reaction, five bentonite-supported zero-valent metal catalysts were prepared by liquid-phase reduction. The results showed that the bentonite-supported zero-valent copper exhibited best catalytic performance, attributed to the high dispersion of active sites of zero-valent copper. The effects of the most relevant operating factors (H2O2 concentration, catalyst dosage, temperature and pH) were evaluated in detail. Moreover, the chemical oxygen demand removal rate of HPGG can achieve 76% when the reaction time was selected at 45 min under optimal experimental conditions. The stability evaluation showed that the catalytic performance was almost unaffected after the catalyst was recycled and used once more showing the good stability of the bentonite-supported zero-valent copper in the application process.
1,2,4-triazoles Clubbed Pyrimidine Compounds with Synthesis, Antimicrobial, Antituberculosis, Antimalarial, and Anti-protozoal Studies
Authors: Patel Navin B. , Soni Hetal I. , Parmar Rahul B. , Chan-Bacab Manuel J. , Rivera Gildardo 
Published: October 2020

:
Triazoles are famous as an antifungal agent. Itraconazole and fluconazole are the best examples of antifungal drugs available in the market, which consist an active triazole moiety. Pyrimidines are also bioactive molecules which shows multiple bioactivity. It’s an effort to synthesize pyrimidine clubbed triazole to enhance bioactivity. To synthesize new active pyrimidine clubbed triazole biomolecule and to evaluate these new products for better drug potential as antimicrobial, an-tituberculosis, antimalarial, and anti-protozoal. N-[4-(substituted phenyl)-6-(substituted aryl) pyrimidine-2-yl]-2-[(4H-1,2,4-triazol-4-yl)amino]acetamide(3A-J) were synthesized by different method as cyclization, condensation, purification and crystallization. The newly synthesized compounds were characterized by IR, 1H NMR, 13C NMR and mass spectral analysis and screened for antibacterial, antifungal, anti-tuberculosis, antimalarial, and anti-protozoal activities. These com-pounds satisfied the bioactive response and simple way for the synthesis.
New Chalcone Derivatives with Pyrazole and Sulfonamide Pharmacophores as Carbonic Anhydrase Inhibitors
Authors: Tugrak Mehtap , Gul Halise Inci , Akincioglu Hulya , Gulcin Ilhami 
Published: October 2020

Background:
Compound containing sulfonamide, pyrazole and chalcone groups are
important in medicinal chemistry. They have a wide range of biological activities, including carbonic
anhydrase (CA) inhibitory activities.

Introduction:
Carbonic anhydrase I and II inhibitors are used for the treatment of diseases, such as
retinal and cerebral edema (CA I), epilepsy, and glaucoma (CA II). However, the currently available
drugs have some limitations or side effects. Thus, there is a need for new drug candidates to
overcome these issues. In this study, a series of compounds, (E)-4-(4-(3-aryl)-3-oxoprop-1-en-1-yl)-
3-phenyl-1H-pyrazol-1-yl) benzenesulfonamides MS4-MS10, were designed to discover new CA
inhibitors using a hybrid approach.

Methods:
Compounds MS4-MS10 were synthesized as shown in Scheme 1, and their chemical
structures were confirmed by 1H NMR, 13C NMR, and HRMS spectra. The CAs (E.C.4.2.1.1) inhibitory
effects of MS4-MS10 were tested on the hCA I and II isoenzymes using previously reported
procedures.

Results:
The CA inhibitors MS4–MS10 gave IC50 values (nM) of 27.8–87.3 towards hCA I and
24.4–54.8 towards hCA II while the IC50 values for reference drug acetazolamide were 384.2 (hCA I)
and 36.9 (hCA II). MS7 and MS9 exhibited 13.8 (hCA I) and 1.5 (hCA II) times more potent CA
inhibition than the reference compound acetazolamide, respectively.

Conclusion:
MS7 (Ar: 2,4,5-trimethoxy phenyl) and MS9 (Ar: 3,4-dimethoxy phenyl) were the
most promising compounds of our series with the lowest IC50 values towards hCA I and hCA II,
respectively, and can be considered for further studies.
Virtual Screening of Chinese Medicine Small Molecule Compounds Targeting SARS-CoV-2 3CL Protease (3CL pro)
Authors: He Qingxiu , Chen Xin , Yang Xi , Li Guangpin , Guo Haiqiong , Chu Han , Lin Zhihua , Wang Yuanqiang 
Published: October 2020

Background:
The outbreak of coronavirus disease 2019 (COVID-19) caused by severe
acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has attracted worldwide attention due to
its high infectivity and pathogenicity.

Objective:
The purpose of this study is to develop drugs with therapeutic potentials for COVID-19.

Methods:
we selected the crystal structure of 3CL pro to perform virtual screening against natural
products in the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform
(TCMSP). Then, molecular dynamics (MD) simulation was carried out to explore the binding
mode between compounds and 3CL pro.

Results and Discussion:
A total of 6 candidates with good theoretical binding affinity to 3CL pro were
identified. The binding mode after MD shows that hydrogen bonding and hydrophobic interaction play
an important role in the binding process. Finally, based on the free binding energy analysis, the candidate
natural product Gypenoside LXXV may bind to 3CL pro with high binding affinity.

Conclusion:
The natural product Gypenoside LXXV may have good potential anti-SARS-COV-2
activity.
Synthesis, Characterization and Biological Studies of Organoselenium trans-Palladium(II) Complexes
Authors: Raković Ivana , Bogojeski Jovana , Mladenović Katarina , Petrović Angelina , Divac Vera , Mihailović Kristina , Jovicić Biljana Popovska , Kostić Marina , Canović Predrag , Milivojević Nevena , Zivanović Marko , Radojević Ivana 
Published: October 2020

Background:
Over the years, transition metal complexes have exhibited significant antimicrobial
and antitumor activity. It all started with cisplatin discovery, but due to the large number of
side effects it shows, there is a growing need to find a new metal-based compound with higher selectivity
and activity on more tumors.

Objectives:
Two novel trans-palladium(II) complexes with organoselenium compounds as ligands,
[Pd(L1)2Cl2] (L1 = 5-(phenylselanylmethyl)-dihydrofuran-2(3H)-one) and [Pd(L2)2Cl2] (L2 = 2-
methyl-5-(phenylselanylmethyl)- tetrahydrofuran) were synthesized, in the text referred to as Pd-Se1
and Pd-Se2. Also, a structurally similar trans-palladium(II) complex, [Pd(L3)2Cl2] (L3= 2,2-
dimethyl-3-(phenylselanylmethyl)-tetrahydro-2H-pyran) was synthesized according to an already
published work and is referred to as Pd-Se3. The interaction of synthesized complexes with DNA and
bovine serum albumin was observed. Also, antimicrobial activity and in vitro testing, cell viability,
and cytotoxic effects of synthesized ligands and complexes on human epithelial colorectal cancer cell
line HCT-116 were studied. Molecular docking simulations were performed to understand better the
binding modes of the complexes reported in this paper with DNA and BSA, as well as to comprehend
their antimicrobial activity.

Methods:
The interactions of the synthesized complexes with DNA and bovine serum albumin were
done using UV-Vis and emission spectral studies as well as docking studies. Antimicrobial activity
was tested by determining the minimum inhibitory concentrations (MIC) and minimum microbicidal
concentration (MMC) using the resazurin microdilution plate method. Cytotoxic activity on cancer
cells was studied by MTT test.

Results:
The Pd(II) complexes showed a significant binding affinity for calf thymus DNA and bovine
serum albumin by UV-Vis and emission spectral studies. The intensity of antimicrobial activity varied
with the complexes Pd-Se1 and Pd-Se3, showing significantly higher activity than the corresponding
ligand. The most significant activity was shown on Pseudomonas aeruginosa. Under standardized
laboratory conditions for in vitro testing, cell viability and cytotoxic effects of synthesized
ligands and complexes were studied on human epithelial colorectal cancer cell line HCT-116, where
Pd-Se2 showed some significant cytotoxic effects.

Conclusion:
The newly synthesized complexes have the potential to be further investigated as metallodrugs.
Design and Synthesis of Novel Anti-inflammatory/Anti-ulcer Hybrid Molecules with Antioxidant Activity
Authors: Chaudhari Bhim Bahadur , Bali Alka , Balaini Ajitesh 
Published: October 2020

Background:
NSAIDs are the most widely prescribed medications worldwide for their
anti-inflammatory, antipyretic, and analgesic effects. However, their chronic use can lead to several
adverse drug events including GI toxicity. The selective COX-2 inhibitors developed as gastrosparing
NSAIDs also suffer from serious adverse effects which limit their efficacy.

Objective:
Local generation of reactive oxygen species is implicated in NSAID-mediated gastric ulceration
and their combination with H2 antagonists like famotidine reduces the risk of ulcers. The
objective of this work was to design and synthesize novel methanesulphonamido isoxazole derivatives
by hybridizing the structural features of NSAIDs with those of antiulcer drugs (ranitidine, famotidine,
etc.) to utilize a dual combination of anti-inflammatory activity and reducing (antioxidant)
potential.

Method:
The designing process utilized three dimensional similarity studies and utilized an isoxazole
core having a potential for anti-inflammatory as well as radical scavenging antioxidant activity.
The compounds were assayed for their anti-inflammatory activity in established in vivo models. The
in vitro antioxidant activity was assessed in potassium ferricyanide reducing power (PFRAP) assay
employing ascorbic acid as the standard drug. Results: Compounds 5, 6, 9 and 10 showed antiinflammatory
activity comparable to the standard drugs and were also found to be non-ulcerogenic at
the test doses. Compounds 6-10 exhibited good antioxidant effect in the concentration range of 1.0-
50.0 μmol/ml. The test compounds were also found to comply with the Lipinski rule suggesting good
oral absorption.

Conclusion:
A new series of isoxazole based compounds is being reported with good antiinflammatory
activity coupled with antioxidant potential as gastro-sparing anti-inflammatory agents.
Method and Device for Production of Driving Force in Underwater Floating Vehicle
Authors: Auzins Janis , Eimanis Marcis 
Published: October 2020

Background:
In this paper, we present a completely new type of propulsion system where
thrust force is created by the helicoidal shape of the hull rather than screw propellers. The method and
device have been recently patented. The inspiration comes from nature, the world of bacteria, where
some organisms have been using the drilling motion or rotating flagella to move in fluid media for several
million years. The hull of device consists of three parts – bow and stern (which create the propulsion
force) and the middle part which can serve as a cargo compartment containing all control mechanisms
and communications. The Cardan-joint mechanism that allows changing the direction of the vehicle
is actuated by bending drives. A bending drive velocity control algorithm is proposed for the automatic
control of vehicle movement direction.

Objective:
Development of a new propulsion system for underwater vehicles.

Methods:
The watercraft dynamics were simulated using multibody simulation software MSC Adams. For accurate
interaction with medium, polynomial metamodels were created on the basis of computer experiments with CFD software.
Computational Fluid Dynamics software was used for flow interaction with the prototype geometry, implementing the
same idea that is used in wind tunnel experiments.

Results and Conclusion:
The results are compared with measurements of the physical prototype, built at the Institute of
Mechanics and Mechanical Engineering of Riga Technical University. These results showed good validation with
mathematical simulation and confirmed the effectiveness and future potential of the proposed principle.