bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forest Structure, Diversity and Regeneration Pattern Along Altitudinal Gradient in Temperate Zone of Nanda Devi Biosphere Reserve (a World Heritage Site), Western Himalaya, India
Authors: Maletha Ajay , Maikhuri Rakesh Kumar , Bargali Surendra Singh 
Published: January 2021

Abstract
BackgroundThe present study exhibited forest structure, plant species composition and regeneration pattern in temperate zone of Nanda Devi Biosphere Reserve (NDBR), western Himalaya along the different altitudinal gradient (2600 to 3600 m asl). The diverse ecosystems of the NDBR contain a tremendous array of floral and faunal diversity, many of which are rare and endangered species. ResultsA total of 223 species of vascular plants (Angiosperm, Gymnosperm and Pteridophytes) were identified within the study area. Rosacaeae (17.69 %) family was exhibited dominant followed by Asteraceae (14.97 %) and Ranunculaceae (12.93 %). Among all the plant species, Betula utilis had highest tree density (724 & 324 individuals ha-1 in each site) and contributed maximum dominance of species cover (44 % and 36 % ) at Tolma and Ghangaria sites followed by Pinus wallichiana (24 %) and Cedrus deodara (15 %), respectively. In Ghangaria site, 56 % tree species showed fair regeneration, 22 % good, 11% exhibited poor and remaining (11 %) indicated no regeneration while at Tolma site, 40 % species showed fair regeneration, 40 % good and remaining 20 % no regeneration. ConclusionOur results suggest to monitor the change in vegetation structure, species composition and regeneration pattern, we should establish permanent study plots in different forest types located along the elevational gradients for an effective and comprehensive monitoring programme to tracks the response of changing climate at both community and species level. Therefore, it is necessary to development of appropriate weather and meteorological station in this sensitive and ecologically important area for regional projection of micro climatic condition and generation of scientific data on changing vegetation composition and advancement of the native species.
Extended Doxycycline Treatment Versus Aspiration of Hydrosalpingeal Fluid in the Management of Patients With Ultrasound Visible Hydrosalpinx Who Refused or Not Eligible to Undergo Salpingectomy Prior to IVF-ET
Authors: Fouda Usama , Elshaer Hesham S. , Sobh Sherin M. , Salah Emad M. , Darweesh Fatma F. , Wali Ahmed A. , Husseiny Tarek El , Taymour Mohammad A. 
Published: January 2021

Abstract
Background: The purpose of this study was to detect whether antibiotic therapy during IVF-ET cycle is as effective as aspiration of hydrosalpinx fluid under ultrasound guidance in preventing the adverse effect of hydrosalpinx on IVF-ET results.Methods: During the period between November 2012 and January 2020, patients with ultrasound visible hydrosalpinges undergoing IVF-ET who refused to undergo laparoscopic salpingectomy or not eligible for laparoscopy were advised to receive antibiotic therapy during IVF-ET cycle or to undergo aspiration of hydrosalpinx fluid under ultrasound guidance at the time of ovum pick up. A retrospective analysis was done for the results of IVF-ET cycles of patients who received antibiotic therapy ( n = 52 ) or underwent aspiration of hydrosalpinx fluid under ultrasound guidance ( n = 76). In the aspiration group, the tip of the suction needle was introduced into the hydrosalpinx to aspirate the hydrosalpinx fluid after completing the ovum pick up. In the antibiotic group, doxycycline (100 mg/12 h) was commenced one week before ovum pick up and was continued for another week after ovum pick up.Results: The implantation, clinical pregnancy, ongoing pregnancy and live birth rates were significantly higher in the aspiration group ( 16.67%% Vs. 8.33%, P value =0.029, 32.89% Vs. 15.38% P value = 0.026, 30.26% Vs. 13.46%,P value =0.028 and 28,95% Vs. 13.46% P value = 0.04 respectively). Conclusion: The aspiration of hydrosalpinx fluid under ultrasound guidance is more effective than antibiotic therapy in preventing the negative impact of hydrosalpinx on the results of IVF-ET. The results of the current study should be proved by subsequent randomized controlled studies.
The Role of Nickel-titanium Shape Memory Alloy Embracing Fixator in Determination of the Implantation Angle of Hip Tumor Prosthesis Stem Following Tumor Resection and the Analysis of Its Efficacy: a Retrospective Study
Authors: Wang Yang , Zhou Panyu , Xia Demeng , Wang Yunyun , Wang Sheng , Zhang Hongyue , Xu Shuogui 
Published: January 2021

Abstract
Background: To explore the role of nickel-titanium(NiTi) shape memory alloy embracing fixator in determination of implantation angle of hip tumor prosthesis stem and analyze its efficacy. Methods: 36 patients with proximal femur tumor were treated with extended tumor resection and prosthetic replacement. 14 patients received prosthetic replacements with the embracing fixators fixing between the junction of the prosthesis stem and the femur temporarily, while the other 22 patients received the same replacements but without the fixators. Following aspects were compared: occurrence of complications, limb function and active hip ROM.Results: There are fewer cases of hip dislocation in the group received prosthetic replacements with the use of embracing fixators. Occurrence of deep infection presented no difference between the two groups. Better limb function and higher active range of motion (ROM) on abduction or flexion were also found in the group using embracing fixators. Conclusion: Ni-Ti shape memory alloy embracing fixator plays a key part in assisting the accurate implantation angle of the prosthesis stem in the prosthetic replacement. The prosthesis stem can be adjusted to the optimal angle with the help of the embracing fixator. Patients have lower chance of dislocation, better limb function, and higher active hip ROM. Trial registration: retrospectively registered.
Comparison of Efficacy and Safety of Duloxetine and Nonsteroidal Anti-inflammatory Drugs in Subacromial Impingement Syndrome
Authors: Zhao Li , Teng Li , Xu Feng , Zhang Huayun , Xie Zhigang , Huang Bin 
Published: January 2021

Abstract
Background: Subacromialimpingement syndrome (SIS) is characterized by shoulder pain and restriction in range of motion (ROM), which lead to debility and decrease quality of life (QoL). Duloxetine could provide persistent long-term pain relief in chronic musculoskeletal pain. Therefore, we aimed to investigate the efficacy of duloxetine in stage I or II SIS patients through comparing the nonsteroidal anti-inflammatory drugs (NSAIDs) treatment.Methods: The patients diagnosed with stage I or II SISwere randomly assigned into the duloxetine group (N= 37) and NSAIDs group (N= 37). Duloxetine group patients started on oral duloxetine 40 mg per day for one week and then titrated up to 60 mg per day for one week. NSAIDs group patients received oral loxoprofen sodium tablets 60mg3 times a day for two weeks. The standard measures for investigating the efficacy include pain intensity (VAS), ROM, shoulder functional status, and the QoL at baseline, end of treatment, and at 1 and 3 months follow-up.Results: 74 eligible patients completed the treatment and evaluation. Both treatment groups improved significantly from baseline over time. And all parameters of pain intensity, shoulder functionalstatus and QoL in the duloxetine group were significantly better than those in the NSAIDs group.And no one manifested SIS recurrence and side effects during the entire follow-up period.Conclusions: Both duloxetine and NSAIDs can be beneficial in the rehabilitation of stage I or II SIS patients. Moreover, duloxetine resulted in improvements in outcomes greater than NSAIDs for the treatment of SIS. The current results indicated that duloxetine treatment might be used as a new safe and effective alternative for SIS. Given the encouraging results of this study, it would be worthwhile to confirm our findings in randomized placebo-controlled multicentre clinical trials.
Fractures of the Lateral Process of the Talus: A New Classification Based on CT
Authors: Wang Yuchuan , Zhu Yanbin , Deng Xiangtian , Wang Zhongzheng , Tian Siyu , Tan Zhanchao , Fu Lei , Ma Jiangtao , Zhang Yingze 
Published: January 2021

Abstract
Background: The common classifications of the fractures of the lateral process of the talus（LTPFs）are based on radiographs and may underestimate the complexity of LTPF, therefore, requiring a comprehensive classification based on CT(Computed tomography) scan. The aim of this study is to propose a such classification system, and to evaluate its reliability and reproducibility.Methods: On the basis of the most widely recognized classifications of Hawkins as well as McCrory-Bladin, we proposed a new and comprehensive classification based on CT scan for the LTPF. We retrospectively reviewed 42 patients involving LTPF. All fractures were classified according to Hawkins, McCrory-Bladin and new proposed classification system by three surgeons. The analysis of interobserver and intraobserver agreements was done using kappa statistics.Results: This new classification included two types based on presence of concomitant injuries or not, with type I consisting of three subtypes and type II of five subtypes. Interobserver and intraobserver reliability of the new classification system were almost perfect (κ=0.846 and 0.823, respectively)，showing a higher interobserver and intraobserver reliability compared to the Hawkins classification (κ=0.737 and 0.689, respectively) as well as McCrory-Bladin classification (κ=0.748 and 0.714, respectively). Conclusion: This new classification system for the LTPF based on CT is a comprehensive classification considering concomitant injuries. It is more reliable and reproducible and can potentially become a useful instrument for decision making of treatment options for LTPFs. Further studies on the evaluation of their clinical relevance (especially the long-term outcome) are warranted.
Insecticide Resistance Status of Anopheles Arabiensis in Irrigated and Non-irrigated Areas in Western Kenya
Authors: Orondo Pauline , Nyanjom Steven , Atieli Harrysone , Githure John , Ondeto Benyl , Ochwedo Kevin , Omondi Collince , Kazura James , Lee Ming-Chieh , Zhou Guofa , Zhong Daibin , Githeko Andrew , Yan Guiyun 
Published: January 2021

Abstract
Background: Malaria control in Kenya is based on case management and vector control using long lasting insecticidal nets (LLINs) and indoor residual spraying (IRS). Irrigation practices maintain vector population and thus transmission during dry season. Development of insecticide resistance further compromises the effectiveness of insecticide-based vector control programs. The aim of this study was to assess the status and mechanism of insecticide resistance in malaria vectors in irrigated and non-irrigated areas in western Kenya and the contribution of public health interventions and agriculture to insecticide resistance. Methodology: The study was carried out in 2018–2019 in Homa Bay County, western Kenya. Anopheline larvae were collected in irrigated and non-irrigated fields, reared to F1 adults and 2-5 day-old female vector mosquitoes were subjected to standard WHO insecticide susceptibility tests. The test specimens were then screened for knock-down resistance, kdr alleles, and analyzed for presence of acetyl-cholinesterase inhibiting enzyme; angiotensin-converting enzyme (Ace-1) genes. All field-collected samples were preserved for species identification by polymerase chain reaction. To ascertain the probable cause of vector resistance to insecticides, a questionnaire was administered to farmers, households and veterinary officers in the study area to assess the use of public health and agricultural insecticides/pesticides.Results: Anopheles arabiensis was the only species tested in irrigated (100%, n=154) area and predominant species in the non-irrigated areas (97.5%, n= 162) and the rest were An. gambiae sensu stricto. In 2018, susceptibility was observed in the vector species in the irrigated area and phenotypic resistance in the non-irrigated area while in 2019, phenotypic resistance was observed from all areas However, susceptibility to malathion (mortality 100%), DDT (98.98%-100%) and PBO- deltamethrin (100%) was observed. Molecular analysis of the vectors from the irrigated and non-irrigated areas revealed low levels of leucine- serine/ phenylalanine substitution at position 1014 (L1014S/ L1014F) with a mutation frequencies of 1%-16%, and almost zero mutation in Ace-1R gene (0.7%). In addition to very high coverage of LLINs impregnated with pyrethroids and IRS with organophosphate insecticides, pyrethroids were the predominant chemical class in pesticides used for crop and animal protection.Conclusion: Extensive use of pyrethroids in agriculture and public health could have resulted in the initial development of insecticide resistance. The susceptibility of these malaria vectors to organophosphates and PBO synergist in pyrethroids offers a promising future for IRS and ITN based vector control interventions.
Genetically-informed behavioral and cognitive interventions may improve management of tinnitus
Authors: Gkouskou Kalliopi , Vlastos Ioannis , Gazouli Maria , Eliopoulos Aristides G. , Prokopakis Emmanuel 
Published: January 2021

Abstract
Introduction: Psychological and behavioral interventions, such as Cognitive Behavioral Treatment (CBT) and Tinnitus Retraining Therapy (TRT), are commonly applied either alone or in combination for the management of tinnitus but selection of the intervention model remains arbitrary. Herein we evaluated the hypothesis that genetic markers may guide the choice of tinnitus treatment towards improved therapeutic outcomes.Results: Patients with subjective idiopathic tinnitus were assigned to either a genetically-informed CBT or TRT intervention protocol based on the status of four single nucleotide polymorphisms (SNPs) of the dopamine and serotonin pathways, namely COMT rs4680, HTR2A rs7997012, HTR2A rs6311, and TPH2 rs4570625, that have been associated with behavioral or cognitive responses (Group A), or to a conventional approach (Group B) in which the choice of treatment was not directed by genotypic data. Tinnitus Handicap Index (THI) scores of perceived tinnitus severity were recorded at the initial and at the fourth session of treatment using appropriate questionnaires. A statistically significant reduction in the THI scores was evident only in the genotype-guided group A (t = 3.03, p = 0.01).Conclusion: Individualized incorporation of genetic information may improve the outcome of behavioral and cognitive interventions in tinnitus.
Identification of An Immune Signature Predicting Prognosis Risk of Patients in Gastric Cancer
Authors: dang wei , Chen Cai , Ruiqiao Zhang , Qingquan Fan , Xiaoling Song , Weimin Wang , Jun Gu 
Published: January 2021

Abstract
Background

Gastric cancer is a common but lethal cancer owing to deficient in effective treatment. Substantial evidences have proved that immune infiltration plays a key role in progression of gastric cancer. This study aimed to establish a signature based on immune related genes that can predict clinical outcomes and therapeutic efficacy.
Methods

The expression data from The Cancer Genome Atlas database and 4617 immune related genes from previously published 160 immune gene sets were collected for development and validation of the signature. Cox proportional hazard regression model was used to construct the signature. The reliability and forecasting ability were evaluated by two independent datasets from GEO.
Results

A gene model consisting of 47 immune related genes was used as our signature. Risk scores were calculated based on the coefficient and the expression level of each gene in this model. The low risk score group had an obviously favorable prognosis than the other group in all cohorts. Both of univariate and multivariate analysis suggested that our immune gene signature was an independent prognostic factor. Single sample gene Set Enrichment Analysis (ssGSEA) revealed that high risk score was associated with high Th17 cell infiltration, low mast cell and pro- angiogenesis immune cell infiltration. More importantly, patients with high risk score presented high tumor mutation burden (TMB), which is an essential element for predicting therapeutic efficacy of immune check point inhibitor.
Conclusion

This signature is a promising tool to predict prognosis and screen out population who can get benefit from immune check point inhibitor.
High BTBD7 Expression Positive is Correlated with SLUG- Predicted Poor Prognosis in Hormone Receptor- Negative Breast Cancer
Authors: Zi-xiong Li , Ze-nan Huang , Luo Hui , Xiong-bin Yang , Yu-lin Wang , Jie-xin Chen , Xiao-kai Ma , wang tiantian , Ling Lin 
Published: January 2021

Abstract
BACKGROUND: Hormone receptor-negative breast cancer (HRNBC), including triple-negative breast cancer (TNBC) and human epidermal growth factor receptor 2 (HER-2) overexpressing breast cancer, is prone to metastasis and has a poor prognosis. BTB/POZ domain-containing protein 7 (Btbd7) is considered to regulate Slug and the epithelial-mesenchymal transition (EMT) process. However, the role of Btbd7 in HRNBC is unclear. This study aimed to investigate the localization and function of Btbd7 in HRNBC as well as its relationship with Slug and the EMT process.METHODS: Bioinformatics analysis was performed to evaluate the effect of the BTBD7 and SLUG genes expression on the prognosis in HRNBC patients. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to identify differentially expressed genes (DEGs) and possible mechanism in breast cancer. Expression of Btbd7 and Slug in 144 HRNBC and 30 benign lesion tissues obtained from the patients was evaluated using immunohistochemistry and immunofluorescence staining. MDA-MB-231 cells were transfected with BTBD7 siRNA and the expression levels of Btbd7, Slug, and key EMT proteins were analyzed. CCK-8 and cell invasion assays were performed to evaluate cell proliferation and invasion in response to BTBD7 silencing.RESULTS: The total positive rate of Btbd7 expression in HRNBC tumor tissue was 66.7% (96/144), which was higher than that in normal adjacent tissue (NAT) (52.1% 75/144 P=0.001) and benign breast lesion tissues (20%, 6/30 P<0.001). TCGA and immunohistochemistry staining both indicated that higher Btbd7 and Slug expression in HRNBC was associated with poor clinicopathologic features and prognosis. Co-expression of Slug and Btbd7 proteins could be found in HRNBC tissue and MDA-MA-231 cells. BTBD7 silencing significantly up-regulated the epithelial marker E-cadherin (P<0.05), down-regulated the mesenchymal markers α-SMA and Slug (P<0.05), and suppressed the proliferation and invasion abilities of cells. GO and KEGG analyses based on 322 DEGs showed that BTBD7 may be associated with generic transcription in breast cancer.CONCLUSION: Higher expression of BTBD7 was a poor prognostic factor in HRNBC patients and BTBD7 silencing inhibited EMT through regulation of Slug expression. BTBD7 might act as a potential molecular target for gene therapy in HRNBC patients.
BRS1 mediates plant redox regulation and cold responses
Authors: Zhang Dongzhi , Zhao Yuqian , Wang Junzhe , Zhao Peng , Xu Shengbao 
Published: January 2021

Abstract
Background: Brassinosteroid-insensitive 1 suppressor 1 (BRS1), is a serine carboxypeptidase that mediates brassinosteroid signaling and participates in multiple developmental processes in Arabidopsis. However, little is known about the precise role of BRS1 in this context. Results: In this study, we analyzed transcriptional and proteomic profiles of Arabidopsis seedlings overexpressing BRS1 and found that this gene is involved in both cold stress responses and redox regulation. Further proteomic evidence shows that BRS1 regulates cell redox by indirectly interacting with cytosolic NADP+-dependent isocitrate dehydrogenase (cICDH). We identified two novel splice products of BRS1, which might play important roles in development and stress responses in plants. Conclusions: This study highlights the role of BRS1 in plant redox regulation and stress responses, which extends our understanding of extracellular serine carboxypeptidases.
Distinct BTK inhibitors differentially induce apoptosis but similarly suppress chemotaxis and lipid accumulation in mantle cell lymphoma
Authors: Liu Zhuojun , Liu Jia , Zhang Tainming , Li Lin , Zhang Shuo , Jia Hao , Xia Yuanshi , Shi Mingxia , Zhang Jing , Yue Shuhua , Chen Xiaofang , Yu Jian 
Published: January 2021

Abstract
Background: The more selective second-generation BTK inhibitors (BTKi) Acalabrutinib and Zanubrutinib and the first-generation BTKi Ibrutinib are highlighted by their clinical effectiveness in mantle cell lymphoma (MCL), however, similarities and differences of their biological and molecular effects on anti-survival of MCL cells induced by these BTKi with distinct binding selectivity against BTK remain largely unknown.Methods: AlamarBlue assays were performed to define cytotoxicity of BTKi against MCL cells, Jeko-1 and Mino. Cleaved PARP and caspase-3 levels were examined by immunoblot analysis to study BTKi-induced apoptotic effects. Biological effects of BTKi on MCL-cell chemotaxis and lipid droplet (LD) accumulation were examined in Jeko-1, Mino and primary MCL cells via Transwell and Stimulated Raman scattering imaging analysis respectively. Enzyme-linked immunoassays were used to determine CCL3 and CCL4 levels in MCL-cell culture supernatants. RNA-seq analyses identified BTKi targets which were validated by quantitative RT-PCR (qRT-PCR) and immunoblot analysis.Results: Acalabrutinib and Zanubrutinib induced moderate apoptosis in Ibrutinib high-sensitive JeKo-1 cells and Ibrutinib low-sensitive Mino cells, which was accompanied by cleaved PARP and caspase-3. Such effects might be caused by the stronger ability of Ibrutinib to upregulate the expression of pro-apoptotic genes, such as HRK, GADD45A, and ATM, in JeKo-1 cells than in Mino cells, and the expression of such apoptotic genes was slightly changed by Acalabrutinib and Zanubrutinib in both JeKo-1 and Mino cells. Further, Acalabrutinib, Zanubrutinib and Ibrutinib reduced MCL-cell chemotaxis with similar efficiency, due to their similar abilities to downmodulate chemokines, such as CCL3 and CCL4. Also, these three BTKi similarly suppressed MCL-cell LD accumulation via downregulating lipogenic factors, DGAT2, SCD, ENPP2 and ACACA without significant differences. Conclusion: BTKi demonstrated differential capacities to induce MCL-cell apoptosis due to their distinct capabilities to regulate the expression of apoptosis-related genes, and similar biological and molecular inhibitory effects on MCL-cell chemotaxis and LD accumulation.