bluuebunny/crossref_metadata_2025_split huggingface dataset:
CHOICE MODERATES RELATIONSHIPS BETWEEN LEVEL AND DURATION OF CARE AND THE HEALTH IMPACT OF CARING FOR AN OLDER PARENT
Authors: Tarter Robin , Hassouneh Dena , Lindauer Allison , Dieckmann Nathan 
Published: December 2019

Abstract
The perception of choice in the caregiving role has emerged as a key theme in qualitative gerontological caregiving research but few studies have examined choice quantitatively. The aim of our study was to test whether perceived choice moderated the relation between level and duration of care and the health impact of caring for a parent over the age of 65. We tested these questions in a series of structural equation models using existing data from the National Alliance for Caregiving, Caregiving in the U.S. 2015 Survey. We found that for adult-child caregivers of parents who reported a lack of choice in taking on the caregiving role, greater responsibilities for assistance with activities of daily living (ADLs) (p&lt;0.01) and instrumental ADLS (p&lt;0.01), and greater time providing care (p&lt;0.05) predicted the negative impact of caregiving on caregiver health. The number of ADLs performed also predicted the emotional stress of caregiving for parents (p&lt;0.01). Conversely, for caregivers who reported that they did have a choice in taking on the caregiving role, level and duration of care were not significantly related to the impact of caregiving on caregiver health, or the emotional stress of caregiving. Women were significantly more likely to report a lack of choice than men (p&lt;0.05). Additional research is needed to explore the meaning of choice, and the ways in which choice may be especially constrained for daughters who care for older adults, in order to develop interventions to ameliorate the potentially deleterious health effects of caregiving on adult-children.
Dynamic, Helminth-Induced Immune Modulation Influences the Outcome of Acute and Chronic Hepatitis B Virus Infection
Authors: Loffredo-Verde Eva , Bhattacharjee Sonakshi , Malo Antje , Festag Julia , Kosinska Anna D , Ringelhan Marc , Rim Sarkar Sabrina , Steiger Katja , Heikenwaelder Mathias , Protzer Ulrike , Prazeres da Costa Clarissa U 
Published: December 2019

Abstract

Background
Chronic hepatitis B develops more frequently in countries with high prevalence of helminth infections. The crosstalk between these 2 major liver-residing pathogens, Schistosoma mansoni and hepatitis B virus (HBV), is barely understood.

Methods
We used state-of-the-art models for both acute and chronic HBV infection to study the pathogen-crosstalk during the different immune phases of schistosome infection.

Results
Although liver pathology caused by schistosome infection was not affected by either acute or chronic HBV infection, S mansoni infection influenced HBV infection outcomes in a phase-dependent manner. Interferon (IFN)-γ secreting, HBV- and schistosome-specific CD8 T cells acted in synergy to reduce HBV-induced pathology during the TH1 phase and chronic phase of schistosomiasis. Consequently, HBV was completely rescued in IFN-γ-deficient or in TH2 phase coinfected mice demonstrating the key role of this cytokine. It is interesting to note that secondary helminth infection on the basis of persistent (chronic) HBV infection increased HBV-specific T-cell frequency and resulted in suppression of virus replication but failed to fully restore T-cell function and eliminate HBV.

Conclusions
Thus, schistosome-induced IFN-γ had a prominent antiviral effect that outcompeted immunosuppressive effects of TH2 cytokines, whereas HBV coinfection did not alter schistosome pathogenicity.
Epidemiology and Clinical Outcomes of Hospitalizations for Acute Respiratory or Febrile Illness and Laboratory-Confirmed Influenza Among Pregnant Women During Six Influenza Seasons, 2010–2016
Authors: Dawood Fatimah S , Garg Shikha , Fink Rebecca V , Russell Margaret L , Regan Annette K , Katz Mark A , Booth Stephanie , Chung Hannah , Klein Nicola P , Kwong Jeffrey C , Levy Avram , Naleway Allison , Riesel Dan , Thompson Mark G , Wyant Brandy E , Fell Deshayne B 
Published: December 2019

Abstract

Background
Pregnant women are at increased risk of seasonal influenza hospitalizations, but data about the epidemiology of severe influenza among pregnant women remain largely limited to pandemics.

Methods
To describe the epidemiology of hospitalizations for acute respiratory infection or febrile illness (ARFI) and influenza-associated ARFI among pregnant women, administrative and electronic health record data were analyzed from retrospective cohorts of pregnant women hospitalized with ARFI who had testing for influenza viruses by reverse-transcription polymerase chain reaction (RT-PCR) in Australia, Canada, Israel, and the United States during 2010–2016.

Results
Of 18 048 ARFI-coded hospitalizations, 1064 (6%) included RT-PCR testing for influenza viruses, 614 (58%) of which were influenza positive. Of 614 influenza-positive ARFI hospitalizations, 35% were in women with low socioeconomic status, 20% with underlying conditions, and 67% in their third trimesters. The median length of influenza-positive hospitalizations was 2 days (interquartile range, 1–4), 18% (95% confidence interval [CI], 15%–21%) resulted in delivery, 10% (95% CI, 8%–12%) included a pneumonia diagnosis, 5% (95% CI, 3%–6%) required intensive care, 2% (95% CI, 1%–3%) included a sepsis diagnosis, and &lt;1% (95% CI, 0%–1%) resulted in respiratory failure.

Conclusions
Our findings characterize seasonal influenza hospitalizations among pregnant women and can inform assessments of the public health and economic impact of seasonal influenza on pregnant women.
Plasmodium falciparum Gametocyte Density and Infectivity in Peripheral Blood and Skin Tissue of Naturally Infected Parasite Carriers in Burkina Faso
Authors: Meibalan Elamaran , Barry Aissata , Gibbins Matthew P , Awandu Shehu , Meerstein-Kessel Lisette , Achcar Fiona , Bopp Selina , Moxon Christopher , Diarra Amidou , Debe Siaka , Ouédraogo Nicolas , Barry-Some Ines , Badoum Emilie S , Fagnima Traoré , Lanke Kjerstin , Gonçalves Bronner P , Bradley John , Wirth Dyann , Drakeley Chris , Guelbeogo Wamdaogo Moussa , Tiono Alfred B , Marti Matthias , Bousema Teun 
Published: December 2019

Abstract

Background
Plasmodium falciparum transmission depends on mature gametocytes that can be ingested by mosquitoes taking a blood meal on human skin. Although gametocyte skin sequestration has long been hypothesized as important contributor to efficient malaria transmission, this has never been formally tested.

Methods
In naturally infected gametocyte carriers from Burkina Faso, we assessed infectivity to mosquitoes by direct skin feeding and membrane feeding. We directly quantified male and female gametocytes and asexual parasites in finger-prick and venous blood samples, skin biopsy samples, and in of mosquitoes that fed on venous blood or directly on skin. Gametocytes were visualized in skin tissue with confocal microscopy.

Results
Although more mosquitoes became infected when feeding directly on skin then when feeding on venous blood (odds ratio, 2.01; 95% confidence interval, 1.21–3.33; P = .007), concentrations of gametocytes were not higher in the subdermal skin vasculature than in other blood compartments; only sparse gametocytes were observed in skin tissue.

Discussion
Our data strongly suggest that there is no significant skin sequestration of P. falciparum gametocytes. Gametocyte densities in peripheral blood are thus informative for predicting onward transmission potential to mosquitoes and can be used to target and monitor malaria elimination initiatives.
Correlates of Nonrandom Patterns of Serotype Switching in Pneumococcus
Authors: Joshi Shreyas S , Al-Mamun Mohammad A , Weinberger Daniel M 
Published: December 2019

Abstract

Background
Pneumococcus is a diverse pathogen, with &gt;90 serotypes, each of which has a distinct polysaccharide capsule. Pneumococci can switch capsules, evading vaccine pressure. Certain serotype pairs are more likely to occur on the same genetic background as a results of serotype switching, but the drivers of these patterns are not well understood.

Methods
We used the PubMLST and Global Pneumococcal Sequencing Project databases to quantify the number of genetic lineages on which different serotype pairs occur together. We also quantified the genetic diversity of each serotype. Regression model were used to evaluate the relationship between shared polysaccharide components and the frequency of serotype co-occurrence and diversity.

Results
A number of serotype pairs occurred together on the same genetic lineage more commonly than expected. Co-occurrence of between-serogroup pairs was more common when both serotypes had glucose as a component of the capsule (and, potentially, glucuronic acid, any-N-acetylated sugar, or ribitol). Diversity also varied markedly by serotype and was associated with the presence of specific sugars in the capsule.

Conclusions
Certain pairs of serotypes are more likely to co-occur on the same genetic background. These patterns were correlated with shared polysaccharide components. This might reflect adaptation of strains to produce capsules with specific characteristics.
Chronic Disseminated Candidiasis During Hematological Malignancies: An Immune Reconstitution Inflammatory Syndrome With Expansion of Pathogen-Specific T Helper Type 1 Cells
Authors: Candon Sophie , Rammaert Blandine , Foray Anne Perrine , Moreira Baptiste , Gallego Hernanz Maria Pilar , Chatenoud Lucienne , Lortholary Olivier 
Published: December 2019

Abstract

Background
Chronic disseminated candidiasis (CDC) is a rare disease that mostly occurs after chemotherapy-induced prolonged neutropenia in patients with hematological malignancies. It is believed to ensue from Candida colonization, breach of the intestinal epithelial barrier, and venous translocation to organs. Fungal blood or liver biopsy cultures are generally negative, suggesting the absence of an ongoing invasive fungal disease.

Methods
To unravel the contribution of the immune system to CDC pathogenesis, we undertook a prospective multicentric exploratory study in 44 CDC patients at diagnosis and 44 matched controls.

Results
Analysis of Candida-specific T-cell responses using enzyme-linked immunospot assays revealed higher numbers of interferon (IFN)γ-producing T cells reactive to mp65 or candidin in 27 CDC cases compared with 33 controls. Increased plasma levels of soluble CD25, interleukin (IL)-6, IL-1β, tumor necrosis factor-α, and IL-10 and lower levels of IL-2 were observed in CDC patients versus controls. Neutrophilia and higher levels of CD4 and CD8 T-cell activation were found in CDC patients as well as increased proportions of CXCR3-expressing TCRγδ +Vδ2+ cells.

Conclusions
The expansion of Candida-specific IFNγ-producing T cells together with features of T-cell activation and systemic inflammation identified here support the view that CDC belongs to the broad spectrum of fungal-associated immune reconstitution inflammatory syndromes.
10 Occurrence of mycotoxins in wheat middlings
Authors: Gott Paige N , Hendel Erika G , Curry Shelby M , Hofstetter Ursula , Murugesan G Raj 
Published: December 2019

Abstract
Mycotoxins are harmful secondary fungal metabolites which can contaminate a variety of feedstuffs worldwide. Various negative effects in vivo are associated with these toxins and severity is dependent on the level, type, and duration of exposure and age, species, and health status of animals. Wheat middlings or “midds” are a by-product of flour milling and are used in a wide range of livestock rations including frequent inclusion in pelleted feeds. The objectives of the current study were to determine the occurrence of mycotoxins in wheat midds samples and to evaluate the potential risk posed to livestock. Since November 2015, 97 samples submitted from 21 states and provinces have been screened via LC-MS/MS technique for the presence of six major mycotoxin groups: aflatoxins, type A trichothecenes, type B trichothecenes (B-Trich), fumonisins, zearalenone, and ochratoxin A (OTA) at Romer Labs (Union, MO, USA) or Activation Laboratories (Ancaster, Ontario, Canada). A subset of samples were also screened for the presence of six ergot alkaloids. Parameters of the main mycotoxins detected are presented in Table 1. On average, 1.7 mycotoxin groups were detected per sample with 91.8% of samples testing positive for at least one mycotoxin. The B-Trich deoxynivalenol was detected in 89.7% of samples (mean 1871.5 ± 157.5 ppb). OTA was also frequently detected (30.9% positive), but at low risk levels (4.2 ± 0.5 ppb). Out of 33 samples screened for ergot alkaloids, 72.7% were positive (493.3 ppb ± 68.3 ppb). Wheat midds are a commonly used feed ingredient for various livestock species. The types and concentrations of mycotoxins detected may pose challenges to livestock health, performance, and reproduction. Special consideration for young animals which are especially susceptible to mycotoxin challenges, such as calves consuming starter feeds, is warranted.
335 Genomic predictions with a multi-breed genomic relationship matrix
Authors: Steyn Yvette , Lourenco Daniela , Misztal Ignacy 
Published: December 2019

Abstract
Multi-breed evaluations have the advantage of increasing the size of the reference population for genomic evaluations and are quite simple; however, combining breeds usually have a negative impact on prediction accuracy. The aim of this study was to evaluate the use of a multi-breed genomic relationship matrix (G), where SNP for each breed are non-shared. The multi-breed G is set assuming known genotypes for one breed and missing genotypes for the remaining breeds. This setup may avoid spurious IBS relationships between breeds and considers breed-specific allele frequencies. This scenario was contrasted to multi-breed evaluations where all SNP are shared, i.e., the same SNP, and to single-breed evaluations. Different SNP densities, namely 9k and 45k, and different effective population sizes (Ne) were tested. Five breeds mimicking recent beef cattle populations that diverged from the same historical population were simulated using different selection criteria. It was assumed that QTL effects were the same over all breeds. For the recent population, generations 1 to 9 had approximately half of the animals genotyped, whereas all 1200 animals were genotyped in generation 10. Genotyped animals in generation 10 were set as validation; therefore, each breed had a validation set. Analysis were performed using single-step GBLUP (ssGBLUP). Prediction accuracy was calculated as correlation between true (T) and genomic estimated (GE) BV. Accuracies of GEBV were lower for the larger Ne and low SNP density. All three scenarios using 45K resulted in similar accuracies, suggesting that the marker density is high enough to account for relationships and linkage disequilibrium with QTL. A shared multi-breed evaluation using 9K resulted in a decrease of accuracy of 0.08 for a smaller Ne and 0.11 for a larger Ne. This loss was mostly avoided when markers were treated as non-shared within the same genomic relationship matrix.
57 Carnosine prevents oxidative damage in myoblast cells derived from porcine skeletal muscle
Authors: Palin Marie-France , Lapointe Jérôme , Gariépy Claude , Beaudry Danièle , Kalbe Claudia 
Published: December 2019

Abstract
Carnosine (β-alanyl-L-histidine) is a molecule naturally and exclusively present in muscle food with the highest concentrations found in skeletal muscles and brain of the animal. Among its numerous biochemical properties, carnosine has antioxidant activity which include metal ion chelation and free radical scavenging. We have recently reported that high muscle carnosine content in pig is associated with better meat quality. Moreover, supplementing pigs with β-alanine reduced oxidative damage to Longissimus muscle (LM) lipids and proteins. Among previously reported antioxidant activities, carnosine was found to limit the production of reactive oxygen species (ROS) and increase antioxidant enzyme activities. However, these studies were mainly conducted in rodents and cell lines and mechanisms in play remain to be characterized. To determine the effect of carnosine in preventing oxidative damage and characterize the mechanisms in play, we have undertaken experiments using the progeny (myoblasts) of satellite cells isolated from the LM of newborn piglets. Cells were treated with carnosine (0, 10, 25 and 50 mM) for 48 h and were then either collected immediately or treated with H2O2 (0.3 mM, 1 h) to induce an oxidative stress. Our results showed that carnosine prevents oxidative stress through the reduction of total intracellular ROS and by modulating the antioxidant system in myoblasts.Carnosine increased the mRNA abundance of NEF2L2, a transcription factor activated by oxidative stress, and several of its downstream regulated antioxidant genes. Western blot analyses further suggest that the protective effect of carnosine on H2O2-induced oxidative stress is mediated through the p38 MAPK intracellular pathway. Finally, the addition of carnosine to H2O2-treated myoblasts increased the basal cellular oxygen consumption rate (OCR), the ATP-linked OCR and proton leaks, thus suggesting an effect of carnosine on mitochondrial functions. Taken together, these findings demonstrate the important role of carnosine in preventing oxidative damage in porcine muscle cells.
253 Glutamate and glutamine are the major metabolic fuels in enterocytes of suckling piglets
Authors: He Wenliang , Hou Yongqing , Wu Guoyao 
Published: December 2019

Abstract
Glutamate and glutamine are known to be important energy substrates in pig enterocytes, and aspartate has also been reported to be extensively catabolized by the rat small intestine. However, little is known about the relative role of amino acids, glucose and fatty acids in ATP production by enterocytes. In the present study, enterocytes isolated from 0-, 7-, 14- and 21-day-old piglets were used to determine the rates of oxidation of amino acids, fatty acids and glucose. Enterocytes were incubated at 37oC for 30 min in Krebs-Henseleit bicarbonate buffer (pH 7.4) containing 5 mM D-glucose and one of the following: 0.5–5 mM L-[U-14C]glutamate, 0.5–5 mM L-[U-14C]glutamine, 0.5–5 mM L-[U-14C]aspartate, 0.5–5 mM L-[U-14C]alanine, 0.5–2 mM L-[U-14C]palmitate, D-[U-14C]glucose, 0.5–5 mM [U-14C]propionate, or 0.5–5 mM [1-14C]butyrate. At the end of the incubation, 14CO2 produced from each 14C-labeled substrate was collected. Rates of oxidation of each substrate in enterocytes from all age groups of piglets increased (P &lt; 0.01) progressively with increasing its extracellular concentrations. The rates of oxidation of glutamate, glutamine, aspartate, glucose by enterocytes from 0- to 21-day-old pigs were much greater (P &lt; 0.01) than those for the same concentrations of alanine, palmitate, propionate, and butyrate. In cells from all age groups of piglets, rates of oxidation of, and ATP production from 5 mM glutamate or 5 mM glutamine were greater (P &lt; 0.01) than those from 5 mM glucose and aspartate. Oxidation of alanine, propionate, butyrate and palmitate by enterocytes was limited. At each postnatal age, the oxidation of glutamate and glutamine produced more ATP than any other substrates. Our results indicated that glutamate and glutamine are the major metabolic fuels in enterocytes of 0- to 21-day-old pigs. Because of limited uptake of arterial glutamate by enterocytes, dietary glutamate is essential to the integrity and function of the pig small intestine.
105 Integrated approach for controlling diseases and parasites in small ruminants: extension education and adoption
Authors: Karki Uma , Karki Lila , Gurung Nar 
Published: December 2019

Abstract
Small-ruminant production is important farm enterprise in Alabama and other states of the Southeast, especially for small and limited-resource farmers. Poor health of animals caused by various diseases and parasites, resulting in a low performance is a major problem faced by most farmers. There are various strategies that can be adopted to minimize the prevalence of diseases and parasites and improve animal health. However, the majority of farmers are lacking the needed knowledge to adopt such strategies. The objective of this program was to educate farmers and extension educators on the integrated approach for controlling diseases and parasites in small ruminants. The program was initiated in 2010 and has been continued with training sessions (n = 7) conducted at the state level. County-level based programming (n = 22) has also been delivered in the form of hands-on training sessions and field days. Moreover, educational materials in the form of workshop proceedings (n = 1) and handouts, flyers, and fact sheets (n = 11) were developed and disseminated to the target audience and public through different outlets (county extension offices, training sessions, field days, farm visits, emails, blog, social media, web pages). Because of this program, numerous small-ruminant farmers and extension professionals are being trained every year (n = 40–51/yr.), and many more have access to educational materials (n = 1,100–1,700/yr.). Short-term impact evaluation of the program shows that knowledge and skills of the trainees have increased significantly (21–62%; P &lt; 0.05). Trainees rated the presented topics useful (94%), they were very likely to implement what they learned (96%), and mentioned that such implementation would improve their farm situation (88%). Several trained farmers were found adopting the integrated approach and educators training their clientele on using the integrated approach to minimize health problems in small ruminants.
254 Digestibility of sheep offered tall fescue, meadow fescue and orchardgrass grasses ensiled with slurry or commercial urea fertilization
Authors: Diaz Jose M , Coffey K P , Coblentz W K , Philipp D , Niyigena Valens , Pruden M C , Myers Douglas D 
Published: December 2019

Abstract
Silage intake by ruminants can be affected by both fertilization and forage type. The objective of this study was to evaluate digestion by sheep offered three different grasses fertilized with either urea (C) or dairy slurry (S). Plots of meadow fescue (MF), tall fescue (TF) or orchardgrass (OG) were planted on 25 July 2017, and first harvested on 29 June 2018. Plots were then fertilized with urea (52 kg N/ha) or slurry (71,150 L/ha). A second harvest was baled 6 August at approximately 57% moisture and wrapped in plastic. Eighteen lambs (59 ± 1.5 kg) were allocated randomly to one of the 6 treatment combinations to provide 3 lambs per treatment within each of 3 periods consisting of a 10-d adaptation and 7-d total fecal and urine collection. Data were analyzed as a 2 × 3 factorial arrangement using PROC MIXED of SAS. Dry matter (DM) and organic matter (OM) intakes (g/kg BW) were greater (P &lt; 0.05) from MF fertilized with C than from the other forage-fertility combinations. Digestibility of DM and OM were greater (P &lt; 0.05) from MF vs. OG, and OG vs. TF. Digestibility of OM also was greater (P &lt; 0.05) from C vs. S. Digestible DM and OM intakes (g/kg BW) were greatest (P &lt; 0.05) from MF fertilized with C. In addition, digestible DM intake (g/kg BW) was greater (P &lt; 0.05) for MF fertilized with S compared to TF fertilized with C, and digestible OM intake was greater (P &lt; 0.05) for MF fertilized with S compared to OG fertilized with S. Therefore, urea-fertilized meadow fescue may improve energy intake compared with that from orchardgrass or tall fescue, but fertilization of meadow fescue with slurry may reduce this advantage. The study was supported in part by USDA-ARS specific cooperative agreement 58-3655-4-052.