bluuebunny/crossref_metadata_2025_split huggingface dataset:
B1 and B2 B cells are characterized by distinct CpG modification states at DNMT3A-maintained enhancers
Authors: Mahajan Vinay S. , Mattoo Hamid , Sun Na , Viswanadham Vinayak , Yuen Grace J. , Allard-Chamard Hugues , Ahmad Maimuna , Murphy Samuel JH , Cariappa Annaiah , Tuncay Yesim , Pillai Shiv 
Published: May 2020

AbstractWe show that DNA methylation is a layered process in B lymphocytes. An underlying foundational methylome is stably established during B lineage commitment and overlaid with a DNMT3A-maintained dynamic methylome which is sculpted in distinct ways in B1 and B2 B cells during B cell development. An engineered loss of DNMT3A after commitment to the B lineage unmasks a foundational methylome that is shared in both B1 and B2 sub-lineages. The dynamic methylome is comprised of novel enhancers whose methylation state is maintained by DNMT3A but can be modulated in strikingly different ways in B1 and B2 B cells. During B1 B cell development, the dynamic methylome undergoes a prominent programmed demethylation event that is not observed during B2 B cell development. The methylation pattern of the dynamic methylome is determined by the coincident recruitment of DNMT3A and TET enzymes and it regulates the developmental expression of B1 and B2 lineage-specific genes.
Permeability of the HIV-1 capsid to metabolites modulates viral DNA synthesis
Authors: Xu Chaoyi , Fischer Douglas K. , Rankovic Sanela , Li Wen , Dick Rob , Runge Brent , Zadorozhnyi Roman , Ahn Jinwoo , Aiken Christopher , Polenova Tatyana , Engelman Alan N. , Ambrose Zandrea , Rousso Itay , Perilla Juan R. 
Published: May 2020

AbstractReverse transcription, an essential event in the HIV-1 lifecycle, requires deoxynucleotide triphosphates (dNTPs) to fuel DNA synthesis, thus requiring penetration of dNTPs into the viral core. The central cavity of the capsid protein (CA) hexamer reveals itself as a plausible channel that allows the passage of dNTPs into assembled capsids. Nevertheless, the molecular mechanism of nucleotide import into the capsid remains unknown. Employing all-atom molecular dynamics simulations, we established that cooperative binding between nucleotides inside a CA hexamer cavity results in energetically-favorable conditions for passive translocation of dNTPs into the HIV-1 capsid. Furthermore, binding of the host cell metabolite inositol hexakisphosphate (IP6) enhances dNTP import, while binding of synthesized molecules like benzenehexacarboxylic acid (BHC) inhibits it. The enhancing effect on reverse transcription by IP6and the consequences of interactions between CA and nucleotides were corroborated using atomic force microscopy, transmission electron microscopy, and virological assays. Collectively, our results provide an atomistic description of the permeability of the HIV-1 capsid to small molecules and reveal a novel mechanism for the involvement of metabolites in HIV-1 capsid stabilization, nucleotide import and reverse transcription.
Temporal Dysbiosis of Infant Nasal Microbiota Relative to Respiratory Syncytial Virus Infection
Authors: Grier Alex , Gill Ann L. , Kessler Haeja A. , Corbett Anthony , Bandyopadhyay Sanjukta , Java James , Holden-Wiltse Jeanne , Falsey Ann R. , Topham David J. , Mariani Thomas J. , Caserta Mary T. , Walsh Edward E. , Gill Steven R. 
Published: May 2020

ABSTRACTRationaleRespiratory Syncytial Virus (RSV) infection is a leading cause of infant respiratory disease and hospitalization. Infant airway microbiota occupying the nasopharynx have been associated with respiratory disease risk and severity. The extent to which interactions between RSV and microbiota occur in the airway, and their impact on respiratory disease severity and infection susceptibility, are not well understood.ObjectivesTo characterize associations between the nasal microbiota and RSV infection before, during, and after infants’ first respiratory illness.MethodsNasal 16S rRNA microbial community profiling of two cohorts of infants in the first year of life: 1) a cross-sectional cohort of 89 RSV infected infants sampled during illness and 102 population matched healthy controls, and 2) an individually matched longitudinal cohort of 12 infants who developed RSV infection and 12 who did not, sampled at time points before, during, and after infection.Measurements and Main ResultsWe identified 12 taxa significantly associated with RSV infection. All 12 were differentially abundant during infection, with seven differentially abundant prior to infection, and eight differentially abundant after infection. Eight of these taxa were associated with disease severity. Nasal microbiota composition was more discriminative of healthy vs. infected than of disease severity.ConclusionsOur findings elucidate the chronology of nasal microbiota dysbiosis and suggest an altered developmental trajectory associated with first-time RSV infection. Microbial temporal dynamics reveal indicators of disease risk, correlates of illness and severity, and the impact of RSV infection on microbiota composition. Identified taxa represent appealing targets for additional translationally-oriented research.
Role of combined cell membrane and wall mechanical properties regulated by polarity signals in cell budding
Authors: Tsai Kevin , Britton Samuel , Nematbakhsh Ali , Zandi Roya , Chen Weitao , Alber Mark 
Published: May 2020

AbstractThe budding yeast,Saccharomyces cerevisiae, is a prime biological model to study mechanisms underlying asymmetric growth. Previous studies have shown that, prior to yeast bud emergence, polarization of a conserved small GTPase, Cdc42, must be established. Additionally, hydrolase changes the mechanical properties of the cell wall and plasma membrane with the periplasm between them (cell surface). However, how the surface mechanical properties in the emerging bud are different from the properties of the mother cell and their role in bud formation are not well understood. We hypothesize that the polarized chemical signal alters the local dimensionless ratio of stretching to bending stiffness of the cell surface of the emerging yeast bud. To test this hypothesis, a novel three-dimensional coarse-grained particle-based model has been developed which describes inhomogeneous mechanical properties of the cell surface. Model simulations suggest that regulation of the dimensionless ratio of stretching to bending stiffness of the cell surface is necessary to initiate bud formation. Furthermore, model simulations predict that bud shape depends strongly on the experimentally observed molecular distribution of the polarized signaling molecule Cdc42, while the neck shape of the emerging bud is strongly impacted by the properties of the chitin and septin ring. This 3D model of asymmetric cell growth can also be used for studying viral budding and other vegetative reproduction processes performed via budding.
Multiplex CRISPRi-Cas9 silencing of planktonic and stage-specific biofilm genes inEnterococcus faecalis
Authors: Afonina Irina , Ong June , Chua Jerome , Lu Timothy , Kline Kimberly A. 
Published: May 2020

ABSTRACTEnterococcus faecalisis an opportunistic pathogen, which can cause multidrug-resistant life-threatening infections. Gaining a complete understanding of enterococcal pathogenesis is a crucial step in identifying a strategy to effectively treat enterococcal infections. However, bacterial pathogenesis is a complex process often involving a combination of genes and multi-level regulation. Compared to established knockout methodologies, CRISPRi approaches enable rapid and efficient silencing of genes to interrogate gene products and pathways involved in pathogenesis. As opposed to traditional gene inactivation approaches, CRISPRi can also be quickly repurposed for multiplexing or used to study essential genes. Here we have developed a novel dual-vector nisin-inducible CRISPRi system inE. faecalisthat can efficiently silence via both non-template and template strand targeting. Since nisin-controlled gene expression system is functional in various Gram-positive bacteria, the developed CRISPRi tool can be extended to other genera. This system can be applied to study essential genes, genes involved in antimicrobial resistance, and genes involved in biofilm formation and persistence. The system is robust, and can be scaled up for high-throughput screens or combinatorial targeting. This tool substantially enhances our ability to study enterococcal biology and pathogenesis, host-bacteria interactions, and inter-species communication.IMPORTANCEEnterococcus faecaliscauses multidrug resistant life-threatening infections, and is often co-isolated with other pathogenic bacteria from polymicrobial biofilm-associated infections. Genetic tools to dissect complex interactions in mixed microbial communities are largely limited to transposon mutagenesis and traditional time- and labour-intensive allelic exchange methods. Built upon streptococcal dCas9, we developed an easily-modifiable, inducible CRISPRi system forE. faecalisthat can efficiently silence single and multiple genes. This system can silence genes involved in biofilm formation, antibiotic resistance, and can be used to interrogate gene essentiality. Uniquely, this tool is optimized to study genes important for biofilm initiation, maturation, and maintenance, and can be used to perturb pre-formed biofilms. This system will be valuable to rapidly and efficiently investigate a wide range of aspects of complex enterococcal biology.
Comparison of total phenolic content and antioxidant activities in selected coloured plants
Authors: Wong Pei Yun , Tan Seok Tyug 
Published: May 2020

PurposeLiterature has consistently reported that coloured plants are rich in dietary bioactive compounds. Therefore, this study aims to compare the total phenolic content and antioxidant activities in selected coloured plants (blue butterfly pea flower, roselle calyx, yellow bell pepper and purple sweet potato).Design/methodology/approachTotal Phenolic Content (TPC) was determined using Folin–Ciocalteu assay, while antioxidant activities were evaluated using 2,2-dophenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging, Ferric Reducing Antioxidant Power (FRAP) and Trolox Equivalent Antioxidant Capacity (TEAC) assays.FindingsTPC was reported from 273.15 ± 19.57 µg GAE/g DW (blue butterfly pea flower extract) to 363.10 ± 7.94 µg GAE/g DW (roselle calyx extract). Antioxidant activities as determined by DPPH assay ranged from 17.26 ± 0.06% (purple sweet potato extract) to 83.38 ± 1.04% (yellow bell pepper extract); while for FRAP assay was 4.92 ± 0.18 mg Fe (II)/g DW (purple sweet potato extract) to 128.33 ± 11.59 mg Fe (II)/g DW (roselle calyx extract). On the other hand, TEAC values were in the range of 15.26 ± 2.83 µg Trolox/g DW (roselle calyx extract) to 364.27 ± 7.14 µg Trolox/g DW (blue butterfly pea flower extract). A significant moderate positive correlation was observed between TPC and DPPH (r = 0.562) as well as TPC and FRAP (r = 0.686).Originality/valueThis study was the first to compare the total phenolic content and antioxidant activities in coloured plants. Findings derived from this study can be extended to the formulation of natural food colourants and nutraceuticals.
Relative export competitiveness of the Cambodian rice sector
Authors: Kea Sokvibol , Li Hua , Shahriar Saleh , Abdullahi Nazir Muhammad 
Published: May 2020

PurposeIn this paper, the authors derive time-varying relative export competitiveness (REC) of the Cambodian rice sector from 1995 to 2018 and examine the key determinants of the REC.Design/methodology/approachThree different REC indexes are calculated in this paper. The authors also developed the relative symmetric export competitiveness (RSEC) index for calculation of comparative advantage. The short-run regression (SRR) model was applied for capturing the determinants of the REC.FindingsThe study results reveal that Cambodia's rice exports became relatively competitive over time. The key findings suggest the Cambodian REC was strengthened as a result of a successful implementation of rice policy and rectangular strategy. The benefits gained from EBA and BRI were found to be the factors contributed to the REC. The higher per capita income had a positive effect on the REC, while higher domestic prices reduced the REC in some phases of the sectoral development.Research limitations/implicationsFurther research is needed in two directions. First, the future studies might focus on other agro-products of Cambodia. Second, the development of the crop-specific factor endowment (CFE) model to consider the effect of endowment factors on the REC could be preferred in light of the data availability.Originality/valueThe research enriches the literature on the agricultural trade and provides a basis for further studies. This work makes a few contributions. First, it is the first study on the REC analysis for the Cambodian rice sector. Second, the latest 24-year data sets were covered. Third, a wide range of comparisons of REC among the world's top rice exporters was provided following implications of the various economic policies and foreign policy strategies, such as RS, EBA and BRI.
Late!: 100% social
Authors: Villalobos Araya Rodrigo 
Published: May 2020

Learning outcomes
The learning outcomes are designing an empresarial strategy and considering different business strategies and environment variables.

Case overview/synopsis
On November of 2015, Eduardo Castillo, General Manager of Late! (B Corporation) programed a directory reunion in which he had to pose a proposal of strategic expansion and growth for the company for future years. A fine of October 2015 publicly revealed the collusion of the two large conglomerates of tissue paper in the Chilean market, with this situation Eduardo took advantage of the discomfort of the consumers and looked alternative brands, he evaluates the launching of a new product: toilet paper Late!. Eduardo had to present at the board meeting the backgrounds to decide the expansion and the strategic growth of the company, taking advantage of the collusion case of the toilet paper or continue their successful project of bottled water.

Complexity academic level
This case focuses primarily on the processes of social enterprise for undergraduate or graduate courses in social entrepreneurship, business model innovation, sustainability, strategic management, emerging markets and business in Chile. Also, this case is also ideal to teach the “business model canvas” and “B Corp.”

Supplementary materials
Teaching Notes are available for educators only.

Subject code
CSS 11: Entrepreneurship.
Tadweer: improving municipal solid waste sustainability practices
Authors: Al Bloushi Badreya Gharib , Ahmad Syed Zamberi , Bani Mfarrej Manar Fawzi 
Published: May 2020

Learning outcomes
To examine and create an ideal pathway model that can implement aiming to change the current improper practices in managing municipal solid waste (MSW) to sustainable practices. To acquire a better understanding of public participation and community culture helps in achieving the aim of reducing the amount of waste generation, sending less waste to landfill sites and encouraging the reuse and recycling of materials instead. To help students whom the awareness in the community regarding the importance of protecting the environment and acting in a civilization way has increased. To improve the MSW sustainability practices and enhance the waste sustainability practices together with energy and material conservation. To have more extensive knowledge and awareness of issues in waste management and some of the dilemmas managers of strategic and operations face.

Case overview/synopsis
Abu Dhabi’s center of waste management is known as Tadweer is a governmental entity under the Abu Dhabi Executive Council. Tadweer is responsible for managing every MSWs includes collecting, transferring, segregating, treating, recycling, reusing and tracking all kinds of wastes. CEO of Tadweer Dr Salem Alkabi called his team that manages various departments such as strategy, operations, projects and licensing. The meeting was to discuss Tadweer’s future directing and strategy for mismanagement of solid waste dumping into landﬁlls in Abu Dhabi. Dumping in landfills is the main challenge Tadweer faced. Mr. Abdulrahman Albloushi’s strategy and business development executive director of Tadweer highlighted to Alkaabi how Tadweer could improve the waste management practices to make it more sustainable. Furthermore, assisting the center gets more benefit from the waste s instead of losing this valuable waste into landfills. Consequently, Mr. Abdulrahman must grapple with some difficult questions: how much the effectiveness in collecting waste from where it generated and removing it out-of-sight?

Complexity academic level
This case study is designed for undergraduate and postgraduate students, and executive MBA students of business management programs, especially for waste management, environmental management and strategic management courses.

Supplementary materials
Teaching Notes are available upon request.

Subject code
CSS 4: Environmental management.
Sunshine Fastech Pvt. Ltd.: working capital financing decision
Authors: Mehta Bhoomi Ruchit 
Published: May 2020

Learning outcomes
The learning outcomes of this paper is to understand the working capital finances offered by bank; comprehend application by the company, loan proposal and bank procedure for additional finance; compute, analyze and interpret financial statements of company and its peers; and assess various factors to be considered while taking loan sanctioning decisions.

Case overview/synopsis
Sunshine had expanded its business by starting in-house manufacturing of a few stages of production of fasteners. Sunshine was in urgent need of additional finance for working capital and had applied to Rajya Bank of India Ltd. (RBIL), requesting to enhance working capital finance limits and other changes. Ruchit Mehta, Relationship Manager of S.G. Highway Branch of RBIL have to assess this request and include his evaluations in the proposal, which he had to present to Assistant General Manager of RBIL.

Complexity academic level
MBA or related program in finance courses such as financial management, corporate finance, financial statement analysis, bank management/finance and training program on “credit management” for bank employees.

Supplementary materials
Teaching Notes are available for educators only.

Subject code
CSS 1: Accounting and Finance
Working with individuals who have experienced homelessness: Stresses and successes
Authors: Rogers Jim , George Thomas , Roberts Amanda 
Published: May 2020

Purpose
Staff who work with vulnerable people with multiple needs are known to experience high levels of stress and burnout, as well as high levels of job satisfaction. This paper aims to explore the experiences of staff working in a project with individuals experiencing long-term homelessness in Lincolnshire.

Design/methodology/approach
An evaluation of a project working with individuals experiencing long-term homelessness included a focus group set up to explore the experiences and well-being of front line staff and managers. One-to-one interviews with staff were also held to provide more in-depth data about the experiences of individual staff members.

Findings
Six key themes were identified from the discussions with participants. Decision latitude and the ability to follow service users on person-centred journeys over long periods were positive aspects of the work which were highly valued and seemed to contribute to high levels of job satisfaction. Things which also made a positive contribution were elements of support provided by the employer and a number of personal coping strategies. Themes identified which had a negative impact on well-being related to high workloads and to the multiple and competing demands from service users and other agencies.

Originality/value
Few studies of any kind have explored the experiences and well-being of staff working in the frontline of homelessness services. This small-scale qualitative study provides a number of suggestions for further research with this population. Findings cannot be generalised but match those of other recent studies which suggest high levels of stress but little evidence of burnout, and that carefully developed workplace support mechanisms play a key role in maintaining commitment to the role and preventing burnout.
Facade inspections with drones–theoretical analysis and exploratory tests
Authors: Falorca Jorge Furtado , Lanzinha João Carlos Gonçalves 
Published: May 2020

PurposeThis paper presents a reflection on the effectiveness of using drones in the technical inspection of building facades, as a practical alternative to more expensive and heavier conventional means. In order to verify this assumption qualitatively, a number of field tests are presented and discussed.Design/methodology/approachThe first stage consisted of a literature review. The analysis initially investigated drone technology. Then, an insight into some general applications in Civil Engineering was compiled, focusing in particular on the Construction Industry (CI), as well as the scope of building envelope inspections. In the second stage, field tests with drones were performed and the resulting data was appraised in order to get facade pathologies diagnosis as accurate as possible.FindingsThrough this study, the adaptability and versatility of using drones were checked. Thus, it has been confirmed that drone technology is an effective and promising alternative methodology to support technical inspection and diagnosis of building envelope pathologies. In fact, drones seem to be a worthwhile tool for supporting certain Civil Engineering activities–particularly when displaying a multirotor configuration, loading high-definition (HD) cameras, for both stills photography and video.Research limitations/implicationsAs the main goal of this approach was simply a qualitative assessment of evidence on the advantages of using drones compared with conventional means, other kinds of comparative analysis were not discussed in depth. However, this and other potential restrictive issues may represent an opportunity to push more developments. A deeper knowledge of data handling and clear procedures for the use of drones could be very valuable for the CI and for building envelope inspections.Practical implicationsIn investigating this subject, this research can offer valuable input to enable a greater awareness of the use of drones in the technical inspection of buildings, particularly in hard-to-reach locations, to the detriment of conventional means. In these circumstances, this methodology can bring great benefits, particularly from an environmental impact point of view, since the resources used are much lower. In addition, a very reliable diagnosis can be achieved more quickly, and at a much lower cost.Originality/valueThe introduction of a standard for drone inspection practice is still at an early stage, with initial steps being taken in an apparently well-focused effort to achieve consolidation. Research studies of this kind may have an important role to play in drawing the stakeholders' attention to the benefits of using this technique, particularly for those working in the building usage phase.