bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complementary transcriptome and proteome analyses provide insight into floral transition in&nbsp;Bamboo&nbsp;(Dendrocalamus latiflorus&nbsp;Munro)
Authors: Wang Xiaoyan , Wang Yujiao , Yang Guoqian , Zhao Lei , Li Dezhu , Guo Zhenhua 
Published: February 2020

Abstract
Background Most woody bamboos flower only once after long vegetable growth phases and die immediately afterward. It is difficult to know the timing of the floral transition, as little information is available on the molecular mechanism of plant maturity in bamboos. Results In this study, through RNA sequencing of leaves of D. latiflorus during floral transition and de novo assembly, a final set of 155,494 unigenes were obtained with N50 of 2,069 bp. We identified a lot of flowering time-associated and flowering integration genes and the continued increase and decrease genes were screened as flowering biomarker genes, such as MADS14, bHLH13, ABA-related genes. The different genes were assigned to specific metabolic pathways by Kyoto Encyclopedia of Genes and Genomes (KEGG) and the photoperiod pathways depending on the circadian rhythm may play an essential role in the bamboo floral transition. In addition, a total of 721 different expressed proteins of leaves from the vegetable-to-reproductive stages in the same flowering clumps were identified using iTRAQ technique. The correlations between the expression levels of a transcript and the abundance of its corresponding protein were observed infrequently, but the very strong correlation in the specific metabolic process was observed, such as carbon metabolism, sugar metabolism, and photosynthesis, underlining the importance of these metabolic pathways during floral transition. Conclusions In this report, we combined transcriptome with large-scale quantitative proteomes to investigate the flower transition of D. latiflorus. This work will provide insights into mechanism of floral transition for bamboos, and management of forest breeding.
Environmental conditions and preventive behaviors’ effect on cutaneous leishmaniasis of earthquake hit western cities of Iran in 2018: a cross-sectional study
Authors: Irandost Mozhgan , Mahaki Behzad , Akbarzadeh Rokhsareh , Pirsaheb Meghdad , Asadi Anvar , Salimi Mojtaba , Chaboksavar Fakhradin 
Published: February 2020

Abstract
Background & objectives This study investigates the role of environmental conditions, knowledge, attitudes and control measures of preventive behaviors in cutaneous leishmaniosis (CL) based on the BASNEF model in patients who referred to the health care centers of cities hit by earthquakes including Sarpol- Zahab and Qasr-Shirin in Kermanshah province, western Iran, in 2018. Method In this descriptive cross-sectional study, 128 individuals who visited rural and urban health centers in the cities under study were randomly selected. They were asked to fill in a structured questionnaire with questions about their demographic information, environmental conditions, their knowledge, attitude and preventive behavior regarding CL. Results The environmental conditions of participants (i.e. wastewater collection, solid waste disposal, drinking water and…) were at appropriate levels. Approximately 89.1% of the people used urban water supply networks while about 68% of participants had access to the wastewater collection system. About 67% of participants reported that their solid wastes were collected daily and disposed of in sanitary landfill. The most influential people in preventive behaviors (control actions) were family members (72.4%). Pearson’s correlation test showed that preventive behaviors had a meaningful correlation with understudy variables in BASNEF model (p<0.001). Preventive behaviors against CL improved as the score of each variable increased. Finally, enabling factors and behavioral intention were the most accurate predictors of preventive behaviors against CL. Conclusion Distribution of causes of CL is influenced by a combination of environmental ecological conditions as well as behavioral and sanitation factors. Therefore, control programs should be focused on villages and cities with high risks of disease.
Oral Status of elderly patients in long stay centres: A systematic review
Authors: Ruiz-Roca Juan Antonio , Martin-Fuentes Dora , Martinez-Beneyto Yolanda , Oñate-Sanchez Ricardo Elias 
Published: February 2020

Abstract
Background: Elderly patients who spend long periods hospitalized or those who are in a situation of institutionalization represent a risk group in this regard, as many of them suffer a degree of dependence and need help to perform the basic tasks of personal care. It is therefore important to learn more of the oral health status of this group of patients in order to make a proper assessment of the situation and to develop protocols for its management.The purpose of the study was to conduct a systematic review to ascertain the oral health status of elderly patients admitted to institutions or hospitalized for a long period of time. Methods: a systematic review of the literature published in two different databases (PubMed, Embase and Cochrane Library) was carried out, with 12 different combinations of keywords based on the following selection criteria: studies published in the last 5 years, in English and/or Spanish and/or Portuguese, with samples of ≥30 patients, performed in patients older than 65 years, admitted to any type of institution and/or hospital center for at least 7 days and in which the state of hard and/or soft tissues of the oral cavity were evaluated in some way. The selected articles were subjected to a thorough analysis. Results: The search strategy covered1 , 014 articles: 689 from Pubmed and 325 from Cochrane Library. After applying the eligibility criteria, fivearticles were selected for our review. The level of evidence of the articles was3, a sample of 773 patients most of them were women with an average age older than 70 years old. Conclusions: the oral health of patients aged more than 65 is worse than that of the rest population. Long hospital stays or being institutionalized in a residence makes this group susceptible to a worsening of their oral health status. It is necessary to develop protocols for the oral health care of these patients, accompanied by training programs for the personnel responsible. Key words: “elderly inpatients”, “elderly hospitalized patients”, “long term hospitalization”, “long term inpatients”, “oral health”, “oral status”.
Protein kinase A-mediated septin7 phosphorylation disrupts septin filaments and ciliogenesis
Authors: Lin Chun-Hsiang , Chen Ting-Yu , Shen Yi-Ru , Wang Chia-Yih , Kuo Pao-Lin 
Published: February 2020

Abstract
Septins are GTP-binding proteins that form heteromeric filaments for proper cell growth and migration. Among these septins, septin7 (SEPT7) is an important component of all septin filaments. Here we showed that protein kinase A (PKA) phosphorylates SEPT7 at Thr197 thus disrupting septin filament dynamics and ciliogenesis. The PKA targeting site at Thr197 of SEPT7 was conserved among species. Treatment of cAMP or overexpression of PKA catalytic subunit (PKACA2) induced SEPT7 phosphorylation. SEPT7 phosphorylation at Thr197 disrupted septin filament formation by reducing SEPT7-SEPT7 interaction, but not affected SEPT7-SEPT6-SEPT2 or SEPT4 interaction. Besides, overexpression of phosphomimetic SEPT7 mutant (T197E) disrupted endogenous SEPT7 filaments suggesting T197E had dominant negative effect on septin filament polymerization. We also identified SEPT7 interacted with the PKACA2 via the GTP-binding domain. Furthermore, PKA-mediated SEPT7 phosphorylation disrupted primary cilia formation. Thus, our data uncover the novel biological function of SEPT7 phosphorylation in septin filament polymerization and primary cilia formation.
Investigation and analysis of the factors of general practice research requirements
Authors: Chao Guanqun , Zhu Yue , Fang Lizheng 
Published: February 2020

Abstract
Background: this study aims to further understand the scientific research ability and level of general practitioners in hospitals at all levels through investigating the general scientific research situation and scientific research intention of general practitioners on duty, so as to provide a basis for formulating scientific research training and scientific research objectives related to general practitioners.Methods: general practitioners working in general medicine departments of hospitals at all levels were selected as the subjects of the survey. Self-filled questionnaires and interviews were used. R software was used for statistical analysis.Results: with the improvement of professional title, the longer time spent in general medicine, the greater interest in scientific research, the more articles published, and the more project funding received.Hospital grade, general practice time, master and doctor degree were independent influencing factors. The higher the hospital grade, the greater the demand for articles, while the longer the general practice, the lower the demand for articles. And the hospital of different rank, promotion needs the requirement of the article to differ. General practitioners who work in tertiary hospitals, have the title of chief physician, master's degree or doctor's degree, are interested in scientific research, have the interest in scientific research training, have the requirement of articles for promotion, have joined the scientific research team and are more inclined to devote their spare time to scientific research. The number of articles published by general practitioners was positively correlated with age, hospital grade, professional title and whether they joined the research team. The number of gp grants was positively correlated with age, hospital grade, professional title and whether or not they joined the research team.Conclusion: hospital grade, general practice time, master's degree and doctor's degree are the correlative factors that affect the requirement of scientific research. Hospital grade, professional title and age are the influencing factors for the scientific research achievements of general medicine, which are positively correlated with the number of articles published and the number of project grants received.
Knowledge and Attitudes Towards Contraceptives Among Adolescents and Young Adults
Authors: Sharma Aanchal , McCabe Edward , Jani Sona , Gonzalez Anthony , Demissie Seleshi , Lee April 
Published: February 2020

Abstract
Background: Despite endorsements supporting the use of intrauterine devices (IUDs) for adolescents and young adult women (AYA), they have limited knowledge about them Male partners can influence contraceptive decisions, however their perceived knowledge about IUDs is lower than their objective knowledge. We aim to establish current AYA baseline contraceptive knowledge and attitudes so providers can better target their sexual health educational interventions.Methods: Females and males, aged 13- 23, from our suburban adolescent clinic, completed an anonymous survey that assessed their knowledge and attitudes towards methods of contraception, with an emphasis on the IUD.Results: Completed surveys totaled 130 (99 females/31 males). Demographics: Black/African-American (31.3%), Latino/Hispanic (30.5%), White (17.6%), Asian (3%), and Other (14.5%). 80% of participants were sexually active. 69.5% stated they/their partner were currently using a contraceptive method; only 2.6% used IUDs. 56.6% of females and 10.1% of males had heard of IUDs. Despite this, male and female participants lacked knowledge regarding specific IUD facts. 6.4% who had used EC knew the copper IUD could be used for EC.Conclusion: Contraceptive knowledge deficits, especially regarding the IUD, continue to exist for AYA patients. Many participants stated they required EC despite “satisfaction” with their birth control method and most were unaware that the copper IUD could be used as EC. These discrepancies highlight the importance of comprehensive contraceptive education for AYA patients. Enhanced and consistent contraceptive options counseling can help providers ensure that their AYA patients make well-informed decisions about family planning, thus improving their quality of life.
Perceptions and experiences of men and women towards acceptability and use of contraceptives in underserved areas of Karachi, Pakistan: A Midline Qualitative Assessment of Sukh Initiative, Karachi Pakistan
Authors: Saleem Sarah , Rizvi Narjis , Feroz Anam , Reza Sayyeda , Jessani Saleem , Abrejo Farina 
Published: February 2020

Abstract
Background: Family planning (FP) is an essential component of Sustainable Development Goals (SDG) and contributes directly to SDG targets 3.7 and 5.6. In Pakistan, contraceptive use has remained stagnant over the past 5 years. This change has been very slow when compared to the FP2020 pledge. The Sukh initiative project was conceived and implemented to alleviate these challenges by providing access to quality contraceptive methods in some underserved areas of Karachi, Pakistan. A qualitative study was been conducted to understand the perceptions and experiences of men and women towards acceptability and contraceptive use.Methods: A qualitative study was conducted at ten Sukh stations located in four towns of Karachi. Focus group discussions (FGDs) were conducted with Married Women of Reproductive Age (MWRA) and married men who received FP services through the Sukh initiative. Study participants were purposively sampled for focus group discussions (FGDs). Interview data was manually transcribed and analyzed using thematic analysis.Results: A total of 20 FDGs(Men=10 FGDs; MWRA=10 FGDs) were conducted. Three overarching themes were identified: (I) Appropriateness and means to promote contraceptive use; (II) Equity and Accessibility to contraceptives; and (III) Perspective on available FP services. Generally, both men and women were informed about FP methods but women were more cognizant of FP information. The Door to Door services by community health workers in Sukh initiative areas was largely appreciated both by women and men as it has made the accessibility and availability of the information and services easy. Women suggested that the Sukh initiative should bring some strategies that can help men broaden their perspective towards FP. The study informed that the men feel left out from the FP programs. Therefore, male participants expressed keen interest in initiatives for men in their communities that would cater to their FP needs.Conclusions: This qualitative study provided a unique opportunity to understand the perceptions of men and women towards the phenomena of contraceptive use. The study identified the need for trained and qualified female and male healthcare providers and well-established health facilities alongside door-to-door services.
Development of the squamate naso-palatal complex: detailed 3D analysis of the vomeronasal organ and nasal cavity in the brown anole Anolis sagrei (Squamata: Iguania)
Authors: Kaczmarek Paweł , Janiszewska Katarzyna , Metscher Brian , Rupik Weronika 
Published: February 2020

Abstract
Background Despite the diverse morphology of the adult squamate naso-palatal complex – consisting of the nasal cavity, vomeronasal organ (VNO), choanal groove, lacrimal duct and soft palate –little is known about the embryology of these structures. Moreover, there are no comprehensive studies concerning development of the nasal cavity and VNO in relation to the soft palate. In this investigation, we used X-ray microtomography and light microscopy to describe embryonic development of the naso-palatal complex of iguanian lizard, the brown anole ( Anolis sagrei ). The purpose of the study was to describe the mechanism of formation of adult morphology in this species, which combines the peculiar anole features with iguanian conditions. Considering the uncertain phylogenetic position of the Iguania within Squamata, embryological data and future comparative studies may shed new light on the evolution of this large squamate clade. Results Development of the naso-palatal complex was divided into three phases: early, middle and late. In the early developmental phase, the vomeronasal pit originates from medial outpocketing of the nasal pit, when the facial prominences are weakly developed. In the middle developmental phase, the following events can be noted: the formation of the frontonasal mass, separation of the vestibulum, appearance of the lacrimal duct, and formation of the choanal groove, which leads to separation of the VNO from the nasal cavity. In late development, the nasal cavity and the VNO attain their adult-like morphology. The lacrimal duct establishes an extensive connection with the choanal groove, which eventually becomes largely separated from the oral cavity. Conclusions Unlike in other tetrapods, the primordium of the lacrimal duct in the brown anole develops largely beyond the nasolacrimal groove. In contrast to previous studies on squamates, the maxillary prominence is found to participate in the initial fusion with the frontonasal mass. Moreover, formation of the choanal groove occurs due to the fusion of the vomerine cushion to the subconchal fold, rather than to the choanal fold. The loss or significant reduction of the lateral nasal concha is secondary. Some features of anole adult-like morphology, such as the closure of the choanal groove, may constitute adaptations to vomeronasal chemoreception.
Factors Affecting Clinical Outcomes in Women with Non-Gastric Gastrointestinal Stromal Tumors
Authors: Huang Peng-Hsuan , Chen Hui-Hwa , Ting Wan-Hwa , Chen Yueh-Yi , Lin Ho-Hsiung , Chen Kuo-Hsin , Hsiao Sheng-Mou 
Published: February 2020

Abstract
Background: Knowledge of the clinical outcome of women with non-gastric gastrointestinal stromal tumors (GISTs) is important for preoperative and postoperative consultation, especially for gynecologic oncologist. The aim of this study is to elucidate the factors affecting the clinical outcome of women with non-gastric GISTs.Methods: Between January 2000 and October 2019, all consecutive women with non-gastric GIST who underwent surgeries in a tertiary referral center were reviewed.Results: Among 26 women with non-gastric GISTs, eight (31%) women had recurrence or metastasis. Common clinical presentations included abdominal pain/fullness (n=12, 46%) and tarry/bloody stool (n=7, 27%). The primary locations of the tumors included the intestines (n=24) and an undetermined origin (n=2). Five (19%) women were initially admitted to the gynecologic department. Twenty-four (92%) patients underwent laparotomic tumor resection, and 2 (8%) patients underwent laparoscopic tumor resection. The probabilities of recurrence-free survival (RFS) at 60 and 120 months were 65.2% and 55.9%, respectively. Death occurred in seven (26.9%) women. The probabilities of overall survival (OS) at 60 and 120 months were 71.1% and 63.9%, respectively. Cancer stage was the only independent predictor for RFS (hazard ratio=6.00, p=0.007) and OS (hazard ratio=3.88, p=0.04). However, excluding cancer stage, metastasis (hazard ratio=8.74 for RFS, 6.03 for OS) and tumor size (hazard ratio=1.20 for OS) were independent predictors. Tumor size ≥ 13.9 cm was the optimum cut-off value to predict death and had an area under the receiver operating characteristic curve of 0.75 (95% confidence interval=0.53 to 0.98).Conclusions: Non-gastric GIST may mimic gynecologic adnexal tumors. In addition to cancer stage, metastasis and tumor size (especially ≥ 13.9 cm for OS) remain independent predictors for RFS and OS in women with non-gastric GIST. The above findings may be used for consultation.Trial registration: ClinicalTrials.gov NCT04256226
Transdermal fentanyl patch provides better pain relief and faster functional recovery after total knee arthroplasty:a meta-analysis
Authors: Wu Jia-qi , Li Yong-mei , Liu Ling-feng , Wu Jian , Mao Lin-bo 
Published: February 2020

Abstract
Background : Pain after total knee arthroplasty (TKA) was severe and should be effectively managed. This meta-analysis aimed to compare transdermal fentanyl patch (TFP) versus placebo for pain control after TKA. Methods : PubMed, Embase, Cochrane library and Chinese National Knowledge Infrastructure (CNKI) were electronically searched. Potential clinical studies that investigated the effect and safety of TFP versus control in TKA patients were searched. The primary outcomes were total morphine consumption. Stata 12.0 was used for meta-analysis. Results : Four randomized controlled trials (RCTs) involving 110 TKA patients were included in this meta-analysis. Compared with control group, TFP was associated with a reduction of total morphine consumption (WMD: -16.14; 95% CI: -25.82 to -6.46; P=0.001). Moreover, TFP could significantly reduce VAS at 2 h, 4 h, 6 h, 12 h, 24 h, 48 h and 72 h (P<0.05). There was no statistically significance between the nausea, vomiting, hypertension, sweating, respiratory depression, pruritus and urine retention (P>0.05). Conclusion : The present meta-analysis showed that use of TFP for the management of moderate or severe TKA postoperative pain had more advantages compared to placebo. Further large-scale, prospective RCTs are required to verify the effect of TFP and optimal dose of TFP in TKA patients.
Bacteriological Properties Of Udder Surface And Milk Samples From Sheep Farms In Hungary
Authors: Tonamo Andualem , Komlósi István , Peles Ferenc , Czeglédi Levente 
Published: February 2020

Abstract
Background Ewe milk due to its beneficial composition and properties contributes to the growth of microorganisms. The primary prerequisite for making high-quality sheep milk product is the production of high-quality raw sheep milk by dairy farms. Thus, the aim of this study was to examine the bacteriological properties of the udder surface (US), individual ewe raw milk (IERM) and bulk tank milk (BTM) samples from sheep farms in Hungary. Methods Seventy-seven US, seventy-seven IERM, and ten BTM samples were examined from March 2018 to April 2019. Total plate count (TPC), Enterobacteriaceae count (EBC), Escherichia coli count (ECC), Staphylococcus aureus count (SAC), lactic acid bacteria count (LAB) and psychrotrophic bacteria count (PBC) of different ewe breeds were examined according to ISO standards in four Hungarian sheep farms. The differences in the microbiological status between the breed and farm were considered. Results High counts of TPC (3.2±1.1 lg cfu/cm 2 ) and EBC (2.4±0.7 lg cfu/cm 2 ) were found in Cigája breed of US samples in farm1. TPC of US sample of Lacaune (farm2), Lacaune (farm3) and British milk sheep was 2.7±0.8, 2.2±0.5 and 2.4±0.4 lg cfu/cm 2 , respectively. The mean value of TPC of IERM of Merino, Cigája and Dorper breeds in farm1 was 2.8±1.2, 3.5±1.2 and 3.3±0.7 lg cfu/ml, respectively. There was significant difference (p<0.05) between Merino and Cigája breeds for TPC. Although there was no significant difference (p<0.05) between breeds for EBC, the mean of EBC of IERM samples from Dorper breed was highest in farm1. Comparatively low TPC of IERM of British milk sheep breed was recorded. The mean of TPC of BTM was 7.4±0.6, 6.3±0.4 and 5.2±0.1 in farm2, 3 and 4, respectively. Both LAB and PBC were high in BTM of Lacaune breed. Except for BTM, SAC and ECC were not detected in most US and IERM samples. Conclusion The presence of microorganisms in BTM above the limit indicates high microbial contamination due to poor hygienic conditions during milking and milk handling. Practicing very good hygiene principles at the farms, in handling and transportation of milk, is a must.