bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymorphism upstream the coding region of the Ppd-D1 gene in Aegilops tauschii Coss.
Authors: Chebotar G. O. , Sechnyak V. E. , Yurganova T. Yu. , Chebotar S. V. 
Published: March 2020

Aim. Evaluate time of heading and flowering in Aegilops tauschii Coss. accessions, donor of the bread wheat D genome, that are differentiated by polymorphism at upstream the coding region of the Ppd-D1 gene. Methods. NA extraction, allele-specific PCR, electrophoresis in polyacrylamide gel, two-factor analysis of variance. Results. We have found polymorphism upstream the coding region of Ppd-D1 gene with the help of the allele-specific PCR with primers to the Ppd-D1b allele in the accessions from the collection of Ae. tauschii Fragment 414 bp was detected in the samples: k-78, k-396, k-529, k-531, k-1144, k-1162, k-1235, k-1273, k-1283, fragment 429 bp have been revealed in the samples k-187, k-968, k-2178, and 453 bp – in the samples k-80, k-2358, Tetra tauschii. The results of a three-year field experiment showed a significant effect of the detected polymorphisms on the time of heading and flowering. Conclusions. Among the Ae. tauschii accessions by allele-specific PCR with primers to the Ppd-D1b allele have been tested amplification fragments 414 bp, 429 bp and 453 bp. Allele Ppd-D1a was not found in the collection of Ae. tauschii accessions. Samples of aegilops that are carrier of insertions 24 + 15 bp (for which the fragment 453 bp was detected by allele-specific PCR) – were characterized by earlier heading and flowering time in certain years.
Keywords: time of heading and flowering, Ppd-D1 gene, Aegilops tauschiі Coss.
Mercury-resistant bacteria in Antarctic ecosystems
Authors: Sioma I. B. , Hovorukha V. M. , Tashyrev O. B. 
Published: March 2020

Aim. The research was focused on the assessment of the possible resistance of Antarctic microorganisms to mercury ions. Methods. Conventional microbiological methods of introducing of decimal dilutions to agar medium. Results. Microorganisms resistant to Hg2+ in high concentrations (up to 500 mg/l) were discovered among samples from Antarctica. The majority of Hg2+-resistant microorganisms was found in moss-lichen and soil ecosystems. The least of Hg2+-resistant microorganisms was found in organics-rich humus ecosystems. Conclusions. In Antarctic microbiocenoses were discovered for the first time microorganisms resistant to mercury in “bactericidal” concentrations in spite of absence of this element in the natural ecosystems. Apparently, the genome of these microorganisms contains genes that provide the mechanisms of microbial resistance to toxic mercury.
Keywords: mercury, metal resistance, Antarctic microorganisms.
Puffing activity in Drosophila melanogaster Meig. political chromosomes after exposure to microwave radiation
Authors: Sheyka M. N. , Strashnyuk V. Yu. 
Published: March 2020

Aim. The aim of the work was to study the effect of microwave radiation of varying intensity on the polytene chromosomes puffing activity in larvae salivary glands of Drosophila melanogaster. Methods. The wild type outbred strain Oregon-R was used as the material. Microwave radiation with a frequency of 36.64 GHz and a power density of 0.1 and 1 W / m2 was used. Exposure to microwaves was applied in early embryogenesis after 3-hour oviposition. Exposure time was 30 sec. The puff sizes were studied on the squashed preparations of larvae salivary glands stained with acetoorcein. Dimensions of four puffs were investigated^ 2B5-6 (X chromosome); 62E, 71CE and 72CD (chromosome 3L). The measurements were carried out using an ocular-micrometer. Results. There were no significant changes in the size of the puffs in any of the four loci studied, regardless of the applied power density. Conclusions. Microwave radiation in early embryogenesis at a frequency of 36.64 GHz, a power density of 0.1 and 1 W/m2, and an exposure of 30 sec does not have a significant effect on the puff sizes in the Drosophila polytene chromosomes.
Keywords: Drosophila melanogaster Meig., giant chromosomes, puff sizes, non-ionizing radiation.
Studying the radioprotective properties of some phytoextracts from Azerbaijan pharmaceutical flora
Authors: Shamilov E. N. , Abdullaev A. S. , Shamilli V. E. , Azizov I. V. 
Published: March 2020

Aim. The aim of this work is to evaluate the radioprotective properties of the extract from the flowers of calendula officinalis (Calendula officinalis L.) alone and phytoextracts (CDY) consisting of calendula officinalis (Calendula officinalis), dandelion officinalis (Taráxacum officinále) and yarrow (Achillea millefolium) obtained from medicinal flora of Azerbaijan. Methods. The medicinal plants included in the collection were dried and the extract obtained in a water-alcohol mixture. The extracts were injected intraperitoneally in mice at a concentration of 300 mg / kg 30 min before irradiation at a dose of 9 Gy. Results. It was found out that the extract of calendula possesses radioprotective activity (55%), somewhat inferior to the activity of the protector-standard of cystamine (80%), and phytoextracts (CDY) decreasing the lethal effect of ionizing radiation, has radioprotective activity (62%) and increases survival and life expectancy lethally irradiated mice more than the extract of calendula (55%) and less cystamine (80%). Conclusions. The data obtained by us testify to the prospects of using the extract of calendula separately and in a complex of extracts of dandelion and yarrow in the development of medicinal compositions intended for preventive purposes and in order to prevent local radiation injuries.
Keywords: mice, survival, irradiation, extract, cystamine.
Hybride necrosis genes in bread wheat varieties (Triticum aestivum L.) of Ukraine
Authors: Galaev A. V. , Galaeva M. V. 
Published: March 2020

Hybrid necrosis is the gradual premature death of leaves or plants in certain F1-2hybrids of wheat (Triticum aestivum L.), and it is caused by the interaction of two dominant complementary genes Ne1 and Ne2 located on chromosome arms 5BL and 2BS, respectively. To date, necrotic genotypes in most varieties of Ukrainian breeding have not been identified. Aim. This study was conducted to determine the necrotic genotypes in varieties Ukrainian breeding of different regions. Methods. Microsatellite analysis, PAAgel-electrophoresis. Results. 150 genotypes of bread wheat varieties from Ukrainian breeding were identified by loci Xbarc74-5В and Xbarc55-2В closely linked to hybrid necrosis genes Ne1 and Ne2, respectively. Conclusions. The most common in the South of Ukraine is the genotypes Ne1wNe2w/m and Ne1mNe2w/m were revealed. The most of varieties in the North of Ukraine had the genotypes ne1Ne2ms. These genotypes can have breeding and adaptive value for specific geographical conditions.
Keywords: Triticum aestivum L., hybride necrosis genes Ne1 and Ne2, microsatellite analysis.
Breeding and genetic patterns in trait expression and efficiency of creation of spring barley cultivars
Authors: Kozachenko M. R. , Vasko N. I. , Naumov O. H. , Solonechnyi P. M. , Solonechna O. V. , Vazhenina O. Ye. , Kompanets K. V. 
Published: March 2020

Aim. Solutions of scientific problems of increasing the efficiency of genetic breeding methods are important objectives in the creation of valuable spring barley cultivars. Methods. Analyses of variance, variation, correlation and regression as well as genetic methods of analyzing features of genotypes in the diallel crossing and top-crossing design were used. Results. The important scientific problems with regard to increasing the efficiency of breeding for yield capacity, brewing quality and starch fraction composition were solved by establishing morphological and biological characteristics, dispersion, correlation, variability, adaptability, breeding and genetic peculiarities of inheritance, heritability, components of genetic variation, combining ability of plant traits as well as the effectiveness of hybridization and mutagenesis methods. As a result of the patterns established, the efficiency of the creation of economically valuable spring barley cultivars was increased. Conclusions. The new solutions of important scientific problems of raising the efficiency of breeding for yield capacity and grain quality were proposed and generalized. On this basis, valuable cultivars were created. As of 2017, 17 of them have been were included in the State Register of Plant Varieties suitable for dissemination inUkraine.
Keywords: breeding-genetic patterns, Hordeum vulgare L., cultivar, breeding method, yield capacity and grain quality.
The value of alien representatives of Prunus L. genus for flowering cherries breeding
Authors: Opalko A. I. , Polishchuk V. V. , Opalko O. A. , Kosar K. P. 
Published: March 2020

Aim. The necessity to search for sources and donors of deficit features for east cherry tree breeding (sakura) and to classify the collection of this promising woody ornamental plant for domestic horticulture determined the conduction of our research. Methods. The availability of some representatives of sakura collection of NDP «Sofiyivka» for breeding was studied with conventional methods, namely, flower color, intensity and duration of flowering, fruit attractiveness, form of a tree crown, vigor and other traits, which determine plant attractiveness for gardens, parks and street plantations, were estimated. Results. Among sakura cultivars which present interest for breeding and are characterized by high ornamental nature along with their adaptability to the conditions of most of the regions in Ukraine, such well-known cultivars as ‘Ama-no-gawa’, ‘Kanzan’, ‘Kiku-shidare-zakura’ and ‘Royal Burgundy’ are to be mentioned. Despite the information concerning the feasibility of spontaneous and the success of artificial intercultivar and interspecific hybridization of Prunus serrulata with other cultivars and other Prunus s. l. genera, at present we have not received P. serrulata hybrids. Conclusions. To enhance the breeding productivity of flowering cherry tree, namely hybridization efficiency of P. serrulata with donors and sources of ornamental traits, it is advisable to involve new initial material on a wide genetic basis not only by economic-valuable features, but also considering the cases of S-genes genetic incompatibility.
Keywords: Amygdaloideae Arn., Prunus sensu lato, initial material for breeding, gametophytic self-incompatibility, oriental flowering cherries (sakura).
Evaluation of toxicity and genotoxicity of nano nanoparticles Ag2S, synthesised by biological matrix, on Drosophila melanogaster Mg. (Diptera: Drosophilidae)
Authors: Protsenko O. V. , Yasinskiy Ya. , Horiunova I. I. , Plohovska S. H. , Borovaya M. N. , Postovoitova A. S. , Pirko N. M. , Pirko Ya. B. , Yemets A. I. , Demydov S. V. , Kozeretska I. A. 
Published: March 2020

Aim. To evaluate the toxicity and genotoxicity of Ag2S quantum dots that had been synthesized by the mycelium of the fungus Pleurotus ostreatus on Drosophila melanogaster. Methods. The toxicity of nanoparticles was determined by assessing the survival rate of imagos and larvae. Genotoxicity was studied in the reparation test. The influence of nanoparticles on the development of the D. melanogaster reproductive system was determined by evaluating the number of ovariols. Results. The toxic effects of ionic silver and the absence of such effects in biosynthetic Ag2S nanoparticles had been shown. The negative influence of biosynthesized Ag2S nanoparticles and biomaterials and ionic silver on the development of D. melanogaster reproductive system had been revealed. There was no genotoxic effect for all investigated specimens. Conclusions. Ag2S biosynthesized quantum dots do not have a toxic and genotoxic effects on D. melanogaster at the concentration of 1.5 mg/ml. However, the action of Ag2S (salt), the biological matrix Pleurotus and biosynthetic Ag2S nanoparticles leads to a decrease in the number of ovariols.
Keywords: Ag2S nanoparticles, D. melanogaster, toxicity, genotoxicity.
DISCRIMINATION À L’EMBAUCHE DES CANDIDATES D’ORIGINE MAGHRÉBINE DANS LA RÉGION DE LA
CAPITALE-NATIONALE
Authors: Brière Simon , Fortin Bernard , Lacroix Guy 
Published: March 2020

Pour des CV semblables en tout point, Samira Benounis recevra-t-elle moins
d’invitations à un entretien d’embauche que Valérie Tremblay dans la région de la
Capitale-Nationale (Québec, Canada) ? Cet article tente de répondre à cette question à
partir d’une expérience utilisant la méthode de testing par envoi de CV fictifs.
Nos résultats suggèrent que, toutes choses égales par ailleurs, la probabilité d’être
invitée à un entretien d’embauche diminue d’environ 10 unités de pourcentage lorsque la
candidate a un nom d’origine maghrébine plutôt que québécoise. Ce constat suggère la
présence d’une discrimination à l’embauche des candidates d’origine maghrébine dans la
région de la Capitale-Nationale.
TROIS ÉVALUATIONS DE POLITIQUE DE CAPITAL HUMAIN VISANT LES JEUNES ENFANTS ET ADOLESCENTS DU QUÉBEC : CONSTATS ET PISTES POUR L’AVENIR
Authors: Merrigan Philip 
Published: March 2020

Dans ce texte, nous présentons les résultats d’évaluations empiriques de trois grandes politiques de capital humain du Québec visant les jeunes enfants et les adolescents : la politique de garde à tarification réduite du gouvernement du Québec, la réforme scolaire du primaire et du secondaire, et la politique de subvention des écoles privées. Nous montrons que les deux premières devront être profondément modifiées pour atteindre leurs objectifs de capital humain, alors que d’autres travaux sont nécessaires pour une évaluation finale de la dernière.
HUBUNGAN BRAND EXPERIENCE, BRAND IMAGE, BRAND SATISFACTION, DAN BRAND LOYALTY DALAM PESPEKTIF FOUR-STAGE LOYALTY MODEL (STUDI TERHADAP MAHASISWA PENGGUNA SMARTPHONE DI TARAKAN)
Authors: Rahmat Rahmat , Marso Marso 
Published: March 2020

Penelitian ini dilakukan untuk menguji pengaruh langsung brand experience terhadap brand image, brand satisfaction, dan brand loyalty. Populasi penelitian ini adalah mahasiswa di Tarakan. Sampel penelitian ini terdiri dari 128 responden yang ditarik dengan metode nonprobability sampling. Software yang digunakan untuk menguji validitas dan reliabilitas instrumen serta menguji hipotesis penelitian ini adalah SmartPLS 3.0 Professional. Hasil penelitian ini menunjukkan bahwa brand experience berpengaruh langsung secara positif dan signifikan terhadap brand image, pengaruh langsung brand experience terhadap brand satisfaction tidak signifikan, pengaruh langsung brand experience terhadap brand loyalty tidak signifikan, brand image berpengaruh langsung secara positif dan signifikan terhadap brand satisfaction, pengaruh langsung brand image terhadap brand loyalty tidak signifikan, dan brand satisfaction berpengaruh langsung secara positif dan signifikan terhadap Brandloyalty. Selain membahas hasil pengujian pengaruh langsung, peran brand image dan brand satisfaction sebagai variabel mediator juga dibahas dalam penelitian ini. Secara teoritis, hasil penelitian ini berkontribusi dalam pengembangan anteseden loyalitas pelanggan; sedangkan secara pragmatis, temuan penelitian ini dapat bermanfaat sebagai bahan dalam merumuskan strategi untuk meningkatkan loyalitas pengguna Smartphone
PENGARUH TAMPILAN WEB DAN HARGA TERHADAP MINAT BELI DENGAN KEPERCAYAAN SEBAGAI INTERVENING VARIABLE PADA E-COMMERCE SHOPEE
Authors: Japarianto Edwin , Adelia Stephanie 
Published: March 2020

E-commerce dipengaruhi oleh berbagai macam faktor, misalnya tampilan web dan harga, serta kepercayaan yang ikut andil dalam mempengaruhi pertumbuhan minat beli online.Dari penelitian ini penulis menemukan bahwa hubungan antara tampilan web dan harga pada minat beli dengan kepercayaan sebagai intervening variable-nya di e-commerce Shopee menunjukkan bahwa kepercayaan merupakan variabel intervening, membuat variabel independen lebih kuat apabila secara langsung berhubungan ke variabel dependen. Responden dalam penelitian ini adalah pengguna aplikasi Shopee yang menggunakan aplikasi Shopee dalam tiga bulan terakhir dan membuka aplikasi Shopee minimal lima kali.
Relationship between circulating levels of cortisol at slaughter and changes of some parameters of the camel meat during ageing
Authors: Abdelilah Lemrhamed Rabab Tabite, , Abbadi Najia El , Belhouari Abderrahmane , Faye Bernard , Khasmi Mohammed El 
Published: March 2020

Relationship between serum levels of cortisol at slaughter and changes of some parameters of meat was investigated in 50 male camels, during ageing for 10 days at 4±1°C. Blood was sampled at slaughter to determine serum levels of cortisol and oblique abdominal external muscle was collected after slaughter to analyze pH, electrical conductivity (EC), drip loss (DL), cooked loss (CL), moisture, solids, ashes, protein total, calcium (Ca), phosphorus (Pi), magnesium (Mg), sodium (Na), potassium (K), catalase activiry (CATa), malondialdehyde (MDA), shrinkage, R-value and total haem pigment (THP) at days 0 (four hours postmortem), 5 and 10 of refrigerated storage. The camels were divided into 3 groups (Gr) with different serum levels of cortisol at slaughter (low, high and very high levels): Gr1 (13.07-67.9 ng/mL, n= 24), Gr2 (80.29-107.21 ng/mL, n= 7) and Gr3 (133.7-198.04 ng/mL, n= 19). Compared to Gr1, Gr2 and Gr3 had a significant (P<0.05) increase of pH, EC, DL, CL, THP, dimensional shrinkage and MDA, and a significant (P<0.05) decrease of CATa at the 5th and 10th postmortem days. However, the bleeding efficiency, moisture, ashes, R-value and levels of Ca, Pi, Na, K and total protein showed no significant differences between twoo cortisol groups. The antemortem circulating levels of cortisol were positively correlated with postmortem pH, EC, DL, CL, THP, dimensional shrinkage and MDA, and negatively correlated with CATa. The analysis of circulating cortisol at slaughtering may predict the quality and oxidant stress status of camel meat during postmortem cold storage.
Hematoma enlargement characteristics in deep versus lobar intracerebral hemorrhage
Authors: Sembill Jochen A. , Kuramatsu Joji B. , Gerner Stefan T. , Sprügel Maximilian I. , Roeder Sebastian S. , Madžar Dominik , Hagen Manuel , Hoelter Philip , Lücking Hannes , Dörfler Arnd , Schwab Stefan , Huttner Hagen B. 
Published: March 2020

AbstractObjectiveHematoma enlargement (HE) is associated with clinical outcomes after supratentorial intracerebral hemorrhage (ICH). This study evaluates whether HE characteristics and association with functional outcome differ in deep versus lobar ICH.MethodsPooled analysis of individual patient data between January 2006 and December 2015 from a German‐wide cohort study (RETRACE, I + II) investigating ICH related to oral anticoagulants (OAC) at 22 participating centers, and from one single‐center registry (UKER‐ICH) investigating non‐OAC‐ICH patients. Altogether, 1954 supratentorial ICH patients were eligible for outcome analyses, which were separately conducted or controlled for OAC, that is, vitamin‐K‐antagonists (VKA, n = 1186) and non‐vitamin‐K‐antagonist‐oral‐anticoagulants (NOAC, n = 107). Confounding was addressed using propensity score matching, cox regression modeling and multivariate modeling. Main outcomes were occurrence, extent, and timing of HE (>33%/>6 mL) and its association with 3‐month functional outcome.ResultsOccurrence of HE was not different after deep versus lobar ICH in patients with non‐OAC‐ICH (39/356 [11.0%] vs. 36/305 [11.8%], P = 0.73), VKA‐ICH (249/681 [36.6%] vs. 183/505 [36.2%], P = 0.91), and NOAC‐ICH (21/69 [30.4%] vs. 12/38 [31.6%], P = 0.90). HE extent did not differ after non‐OAC‐ICH (deep:+59% [40–122] vs. lobar:+74% [37–124], P = 0.65), but both patients with VKA‐ICH and NOAC‐ICH showed greater HE extent after deep ICH [VKA‐ICH, deep: +94% [54–199] vs. lobar: +56% [35–116], P < 0.001; NOAC‐ICH, deep: +74% [56–123] vs. lobar: +40% [21–49], P = 0.001). Deep compared to lobar ICH patients had higher HE hazard during first 13.5 h after onset (Hazard ratio [HR]: 1.85 [1.03–3.31], P = 0.04), followed by lower hazard (13.5–26.5 h, HR: 0.46 [0.23–0.89], P = 0.02), and equal hazard thereafter (HR: 0.96 [0.56–1.65], P = 0.89). Odds ratio for unfavorable outcome was higher after HE in deep (4.31 [2.71–6.86], P < 0.001) versus lobar ICH (2.82 [1.71–4.66], P < 0.001), and only significant after small‐medium (1st volume‐quarter, deep: 3.09 [1.52–6.29], P < 0.01; lobar: 3.86 [1.35–11.04], P = 0.01) as opposed to large‐sized ICH (4th volume‐quarter, deep: 1.09 [0.13–9.20], P = 0.94; lobar: 2.24 [0.72–7.04], P = 0.17).InterpretationHE occurrence does not differ among deep and lobar ICH. However, compared to lobar ICH, HE after deep ICH is of greater extent in OAC‐ICH, occurs earlier and may be of greater clinical relevance. Overall, clinical significance is more apparent after small–medium compared to large‐sized bleedings.
Multiplex SERS Detection of Metabolic Alterations in Tumor Extracellular Media
Authors: Plou Javier , García Isabel , Charconnet Mathias , Astobiza Ianire , García‐Astrain Clara , Matricardi Cristiano , Mihi Agustín , Carracedo Arkaitz , Liz‐Marzán Luis M. 
Published: March 2020

AbstractThe composition and intercellular interactions of tumor cells in the tissues dictate the biochemical and metabolic properties of the tumor microenvironment. The metabolic rewiring has a profound impact on the properties of the microenvironment, to an extent that monitoring such perturbations could harbor diagnostic and therapeutic relevance. A growing interest in these phenomena has inspired the development of novel technologies with sufficient sensitivity and resolution to monitor metabolic alterations in the tumor microenvironment. In this context, surface‐enhanced Raman scattering (SERS) can be used for the label‐free detection and imaging of diverse molecules of interest among extracellular components. Herein, the application of nanostructured plasmonic substrates comprising Au nanoparticles, self‐assembled as ordered superlattices, to the precise SERS detection of selected tumor metabolites, is presented. The potential of this technology is first demonstrated through the analysis of kynurenine, a secreted immunomodulatory derivative of the tumor metabolism and the related molecules tryptophan and purine derivatives. SERS facilitates the unambiguous identification of trace metabolites and allows the multiplex detection of their characteristic fingerprints under different conditions. Finally, the effective plasmonic SERS substrate is combined with a hydrogel‐based three‐dimensional cancer model, which recreates the tumor microenvironment, for the real‐time imaging of metabolite alterations and cytotoxic effects on tumor cells.
Nitric Oxide Release Device for Remote‐Controlled Cancer Therapy by Wireless Charging
Authors: Li Bin , Ji Ping , Peng Si‐Yuan , Pan Pei , Zheng Di‐Wei , Li Chu‐Xin , Sun Yun‐Xia , Zhang Xian‐Zheng 
Published: March 2020

AbstractTraditional phototherapies face the issue that the insufficient penetration of light means it is difficult to reach deep lesions, which greatly reduces the feasibility of cancer therapy. Here, an implantable nitric oxide (NO)‐release device is developed to achieve long‐term, long‐distance, remote‐controllable gas therapy for cancer. The device consists of a wirelessly powered light‐emitting diode (wLED) and S‐nitrosoglutathione encapsulated with poly(dimethylsiloxane) (PDMS), obtaining the NO‐release wLED (NO‐wLED). It is found that NO release from the NO‐wLED can be triggered by wireless charging and the concentration of produced NO reaches 0.43 × 10−6 m min−1, which can achieve a killing effect on cancer cells. In vivo anticancer experiments exhibit obvious inhibitory effect on the growth of orthotopic cancer when the implanted NO‐wLED is irradiated by wireless charging. In addition, recurrence of cancer can be prevented by NO produced from the NO‐wLED after surgery. By illumination in the body, this strategy overcomes the poor penetration and long‐wavelength dependence of traditional phototherapies, which also provides a promising approach for in vivo gas therapy remote‐controlled by wireless charging.