bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of high‐throughput digital lateral flow immunoassays for the detection of influenza A/B viruses from clinical swab samples
Authors: Yoo Soo J. , Shim Hee S. , Yoon Sumi , Moon Hee‐Won 
Published: November 2019

AbstractWe evaluated the performance of new high‐throughput digital lateral flow immunoassays (LFIAs) detecting influenza antigens and compared them with those of the widely used digital LFIA and the rapid nucleic acid amplification test (NAAT). We tested 199 clinical nasopharyngeal (nasal) swab samples using three LFIA tests (BD Veritor Plus, STANDARD F Influenza A/B FIA, and ichroma TRIAS) and the rapid NAAT (ID NOW Influenza A & B2). Agreements and clinical performances (sensitivity and specificity) were evaluated based on the results of reverse transcriptase‐polymerase chain reaction (RT‐PCR) and verification panel. The agreement of each test with RT‐PCR was moderate to almost perfect. The sensitivity of ID NOW was significantly higher than that of LFIAs (P = .0005, .0044, and .0026 for influenza A and P = .0044, .0026, and .0044 for influenza B, respectively). The specificities were not significantly different between the four tests (P > .05). However, the reference panel suggests that ichroma TRIAS test is more sensitive than the other two LFIA tests. All three LFIA assays performed similarly with no false positives against influenza A. For influenza B, ichroma TRIAS had 2 of 166 false positives whereas there were no false positives for the other two LFIA tests. Influenza antigen digital LFIAs have advantages in terms of the workflow when simultaneous tests are required. Rapid NAAT has higher sensitivity, while new antigen LFIAs are efficient and high‐throughput. It is recommended that users select appropriate methods and algorithms according to the number of specimens and laboratory conditions in each clinical laboratory.
Diversity of filamentous fungi associated with coral and sponges in coastal reefs of northeast Brazil
Authors: Paulino Gustavo V. B. , Félix Ciro R. , Landell Melissa F. 
Published: November 2019

AbstractFungi are known to form associations with various marine organisms and substrata such as sponges and corals, both as potential symbionts or pathogens. These microorganisms occupy an ecological niche that has recently attracted great attention due to their potential in either ecological or pharmaceutical advances. However, the interaction between marine invertebrates and fungi is still poorly understood, including how they are affected by anthropogenic actions. Here, we identified 89 fungal isolates through sequencing of the ITS rDNA region obtained from the various sponge and coral species collected at two northeast Brazilian reefs. We found 43 species of fungi from 16 genera, all belonging to phylum Ascomycota. The sponges and coral shared four genera: Aspergillus, Penicillium, Trichoderma, and Cladosporium, all commonly found in terrestrial habitats and associated with marine invertebrates. We observed some unusual species in relation to the marine environment, such as Clonostachys rosea and Neopestalotiopsis clavispora, most of them related to plants, either as saprophytic or pathogenic, suggesting that these species were transported from the surrounding terrestrial environment to the reefs. In addition, some isolates represent possible undescribed species, reinforcing the importance of studying the marine environment in relation to its ecological and biotechnological importance.
The MRL/MpJ Mouse Strain Is Not Protected From Muscle Atrophy and Weakness After Rotator Cuff Tear
Authors: Talarek Jeffrey R. , Piacentini Alex N. , Konja Alexis C. , Wada Susumu , Swanson Jacob B. , Nussenzweig Samuel C. , Dines Joshua S. , Rodeo Scott A. , Mendias Christopher L. 
Published: November 2019

ABSTRACTChronic rotator cuff tears are a common source of shoulder pain and disability. Patients with rotator cuff tears often have substantial weakness, fibrosis, and fat accumulation, which limit successful surgical repair and postoperative rehabilitation. The Murphy Roths Large (MRL) strain of mice have demonstrated superior healing and protection against pathological changes in several disease and injury conditions. We tested the hypothesis that, compared with the commonly used C57Bl/6 (B6) strain, MRL mice would have less muscle fiber atrophy and fat accumulation, and be protected against the loss in force production that occurs after cuff tear. Adult male B6 and MRL mice were subjected to a rotator cuff tear, and changes in muscle fiber contractility and histology were measured. RNA sequencing and shotgun metabolomics and lipidomics were also performed. The muscles were harvested one month after tear. B6 and MRL mice had a 40% reduction in relative muscle force production after rotator cuff tear. RNA sequencing identified an increase in fibrosis‐associated genes and a reduction in mitochondrial metabolism genes. The markers of glycolytic metabolism increased in B6 mice, while MRL mice appeared to increase amino acid metabolism after tear. There was an accumulation of lipid after injury, although there was a divergent response between B6 and MRL mice in the types of lipid species that accrued. There were strain‐specific differences between the transcriptome, metabolome, and lipidome of B6 and MRL mice, but these differences did not protect MRL mice from weakness and pathological changes after rotator cuff tear. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:811‐822, 2020
Development and Application of a Novel Metric to Characterize Comprehensive Range of Motion of Reverse Total Shoulder Arthroplasty
Authors: Elwell Josie A. , Athwal George S. , Willing Ryan 
Published: November 2019

ABSTRACTActive range of motion (ROM) of reverse total shoulder arthroplasty (rTSA) can be limited by bony impingement, muscle inability, and joint instability. The aim of this study was to develop a novel metric representative of comprehensive ROM of rTSA, which is evaluated in the context of all three factors. It was hypothesized that the metric, termed global circumduction ROM (GC‐ROM), would capture differences resulting from directional changes in rTSA design parameters known to increase ROM. GC‐ROM was calculated for a set of 18 rTSA configurations with humeral polyethylene cup depths of 6 and 8.1 mm, glenosphere lateralization (GLat) distances of 0, 5, and 10 mm, and neck‐shaft angles (NSA) of 135°, 145°, and 155°. For any implant configuration, arm positions were defined by internal/external (IE) rotation angle and two spherical coordinates representing the elevation plane angle and elevation angle. At each IE rotation angle, incremental positions with variable elevation plane and elevation angles were checked for feasibility based on impingement, muscle ability, and risk of instability. Coordinates of feasible positions were mapped to unit spheres and connected to form regions, of which the surface area was calculated to represent allowable circumduction ROM. ROMs were averaged across all IE rotation angles to produce a single metric, GC‐ROM. The results showed that decreasing cup depth and increasing GLat and NSA increased GC‐ROM. In conclusion, a novel metric to characterize comprehensive ROM, evaluated based on several ROM‐limiting factors, was developed as a performance metric through which rTSA designs can be compared. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:880‐887, 2020
Determination, residue analysis, risk assessment and processing factors of tebufenozide in okra fruits under field conditions
Authors: Luo Xiaoshuang , Qin Xinxian , Chen Dan , Liu Zhengyi , Zhang Kankan , Hu Deyu 
Published: November 2019

AbstractBACKGROUNDEnsuring the yield, quality, and profitability of okra by preventing and controlling pests with the application of insecticides has increased in the last decade. Some insecticide residues might remain in edible parts of okra (fruits) and lead to several potential human health problems. Therefore, research on the residue behaviour, risk assessment and removal approach of insecticides on okra fruits is important for food safety, together with the proper application and residual elimination of insecticides in okra.RESULTSA simple liquid chromatography with tandem mass spectrometry (LC–MS/MS) method was established and validated for determining the tebufenozide residues in okra fruits. The recoveries of tebufenozide in okra fruits were >72% with relative standard deviations of 0.6 to 6.1%. The dissipation rates of tebufenozide were different in okra fruits cultivated under open land and glasshouse field conditions because of the discriminating humidity and temperature conditions. The dietary intake of the tebufenozide residues from okra fruit consumption for Chinese consumers was fairly low, with approximately no potential health risk. The processing factor values of washing, blanching, washing + blanching and soaking were all less than one, which indicated that these processes could effectively reduce the residual hydrazide in the okra fruit.CONCLUSIONThe developed method for analysing tebufenozide in okra fruits was applicable for field studies on this insecticide. The potential health risk of tebufenozide in okra fruits could be negligible to the health of different age groups of Chinese consumers. The soaking process effectively removed tebufenozide residues from okra fruits. The obtained data will help Chinese governments establish a maximum residue limit of tebufenozide in okra and provide data for the risk assessment and removal of tebufenozide in other crops. © 2019 Society of Chemical Industry
Identification and characterization of antioxidant peptides from Chinese dry‐cured mutton ham
Authors: Wang Jingyun , Lu Shiling , Li Ruiting , Wang Yuan , Huang Liyuan 
Published: November 2019

AbstractBACKGROUNDChinese mutton ham is a dry‐cured meat product with a long ripening time. The aim of this study was to identify and characterize antioxidant peptides from Chinese mutton ham.RESULTSMutton ham peptides (MHPs) were purified by gel filtration, anion exchange and reversed‐phase high‐performance liquid chromatography steps. The 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt (ABTS) free radical scavenging capacity was used to guide the purification of MHPs. Three antioxidant peptides were identified by liquid chromatography with tandem mass spectrometry (LC–MS/MS) as Met‐Trp‐Thr‐Asp (MWTD), Ala‐Pro‐Tyr‐Met‐Met (APYMM) and Phe‐Trp‐Ile‐Ile‐Glu (FWIIE), with molecular weights 551.61, 611.76, and 706.84 Da, respectively. Among them, APYMM exhibited the highest ABTS radical scavenging activity. The three peptides had the ability to inhibit lipid oxidation and Fenton's reagent‐induced protein oxidation and DNA damage. After simulated gastrointestinal digestion, FWIIE and APYMM showed increased antioxidant activity, while MWTD showed decreased activity.CONCLUSIONThree novel peptides isolated from Chinese mutton ham had strong biological activity. Chinese mutton ham is potentially a functional food and an excellent source of natural antioxidants. © 2019 Society of Chemical Industry
Temperature sensitivity of decomposition of soil organic matter fractions increases with their turnover time
Authors: Jia Yufu , Kuzyakov Yakov , Wang Guoan , Tan Wenbing , Zhu Biao , Feng Xiaojuan 
Published: November 2019

AbstractSoil organic carbon (SOC) is an indicator of soil fertility. Global warming accelerates SOC decomposition, consequently, resulting in land degradation. Characterization of the response of SOC decomposition to temperature is important for predicting land development. A simulation model based on temperature sensitivity (Q10) of SOC decomposition has been used to predict SOC response to climate warming. However, uncertain Q10 leads to substantial uncertainties in the predictions. A major uncertainty comes from the interference of rainfall. To minimize this interference, we sampled surface (0–5 cm) soils along an isohyet across a temperature gradient in the Qinghai–Tibetan Plateau. The Q10 of bulk soil and the four soil fractions, such as light fraction (LightF), particulate organic matter (POM), hydrolyzable fraction (HydrolysF), and recalcitrant fraction (RecalcitF), were studied by 14C dating. Turnover time follows the order: LightF < POM < bulk soil < HydrolysF < RecalcitF. The Q10 follows the order: LightF (1.0) = POM (1.0) < HydrolysF (3.63) < bulk soil (5.93) < RecalcitF (7.46). This indicates that stable fractions are much more sensitive to temperature than labile fractions. We also suggest that protection mechanisms rather than molecular composition regulate SOC turnover. A new concept 'protection sensitivity' of SOC decomposition was proposed. Protection sensitivity relates to protection type and primarily controls Q10 variation. A simulation model based on the Q10 of individual fractions predicted SOC change and land development in the Qinghai–Tibetan Plateau in the next 100 years much effectively as compared to simulations based on one‐pool model (Q10 = 2) or bulk soil (Q10 = 5.93).
Structural changes of the microplankton community following a pulse of inorganic nitrogen in a eutrophic river
Authors: Kleinteich Julia , Hilt Sabine , Hoppe Andreas , Zarfl Christiane 
Published: November 2019

AbstractGlobal change will increase the number and severity of extreme events resulting in strongly pulsed nutrient loading to rivers. Recent studies indicate the potential for rapid plankton shifts as short‐term response to storm events and highlight the need for high‐frequency methods to understand these complex processes. Here, we studied the effects of a strong short‐term pulse of ammonium nitrate in a eutrophic temperate river that elevated nitrate‐N concentrations from 3.1 to 10–55 mg L−1. An intense phytoplankton bloom increased chlorophyll a concentrations from below 10 μg L−1 to over 140 μg L−1 despite of high background N concentrations and followed the N‐pulse downstream. High‐throughput sequencing revealed the temporal dynamics of the bacterial (16S rRNA gene) and microeukaryotic (18S rRNA gene) community throughout the event. Bacterial and microeukaryotic community richness was reduced, and community composition changed significantly during the bloom. Few microeukaryote taxa (e.g., Cyclotella meneghiniana, Chlamydomonas) dominated the bloom and replaced the rich summer phytoplankton community. Long‐term monitoring data (2008–2016) using classical microscope analyses showed that seasonal shifts in phytoplankton community composition were linked to physical parameters and nutrient availability with P‐limitation observed in spring but not in summer. We conclude that a disturbance in abiotic and biotic control factors, such as caused by a nutrient pulse, can induce structural shifts in the microplankton community leading to a phytoplankton bloom, even in eutrophic waters.
Cash, credit, or phone? An empirical study on the adoption of mobile payments in the United States
Authors: Zhang Jing , Mao En 
Published: November 2019

AbstractThe purpose of this study is to investigate the effects of consumer factors on behavioral intention to adopt mobile payments. We focus on the proximity mobile payment, which is made feasible by the near‐field communications (NFCs) technology. Building upon the theory of reasoned action and technology acceptance model (TAM), a behavioral intention model was established consisting of enhanced cognitive antecedents as well as affective and social antecedents. Cognitive antecedents encompass the factors including relative advantage, perceived usefulness and ease of use in the TAM, and technology characteristics (e.g., responsiveness and mobility); affective antecedents focus on positive and negative emotions associated with using NFC mobile payments. Both antecedents are expected to affect attitudes. In addition, social antecedents examine subjective norms and the influence of network externalities. Data were collected from 394 adult nonusers of NFC mobile payments in the United States via an online survey. The research model was tested using structural equation modeling. The results showed that all three antecedents significantly affected individual consumers’ intention to adopt NFC mobile payments, explaining a significant amount of variance. Both theoretical and marketing implications are discussed in the context of NFC mobile payments.
Thermodynamic Analysis of the Uptake of a Protein in a Spherical Polyelectrolyte Brush
Authors: Walkowiak Jacek , Lu Yan , Gradzielski Michael , Zauscher Stefan , Ballauff Matthias 
Published: November 2019

AbstractA thermodynamic study of the adsorption of Human Serum Albumin (HSA) onto spherical polyelectrolyte brushes (SPBs) by isothermal titration calorimetry (ITC) is presented. The SPBs are composed of a solid polystyrene core bearing long chains of poly(acrylic acid). ITC measurements done at different temperatures and ionic strengths lead to a full set of thermodynamicbinding constants together with the enthalpies and entropies of binding. The adsorption of HSA onto SPBs is described with a two‐step model. The free energy of binding ΔGb depends only weakly on temperature because of a marked compensation of enthalpy by entropy. Studies of the adsorbed HSA by Fourier transform infrared spectroscopy (FT‐IR) demonstrate no significant disturbance in the secondary structure of the protein. The quantitative analysis demonstrates that counterion release is the major driving force for adsorption in a process where proteins become multivalent counterions of the polyelectrolyte chains upon adsorption. A comparison with the analysis of other sets of data related to the binding of HSA to polyelectrolytes demonstrates that the cancellation of enthalpy and entropy is a general phenomenon that always accompanies the binding of proteins to polyelectrolytes dominated by counterion release.
Reconfigurable wide band wearable multiple input multiple output antenna with hanging resonator
Authors: Biswas Ashim Kumar , Chakraborty Ujjal 
Published: November 2019

AbstractThis letter presents a two‐element textile‐based multiple input multiple output (MIMO) antenna with switchable dual wide band characteristics. The antenna is integrated with two switches enabled hanging resonators and a partial ground plane with funnel‐shaped DGS. It covers the spectra from 3.11 to 5.15 GHz in one and 4.81 to 7.39 GHz in another switching condition. The antenna satisfies the WiMAX/C‐band downlink (3.2‐3.8/3.7‐4.2 GHz) and WLAN/C‐band uplink (5.15‐5.35/5.72‐5.825/5.925‐6.425 GHz) application bands. The resonator structures provide very high port isolation of >19.5 and >21 dB in the lower and upper band, respectively. The proposed antenna offers suitable values of diversity parameters which include envelope correlation coefficient (ECC < 0.18), diversity gain (DG > 9.9 dB), and channel capacity loss (CCL < 0.2 BPS/Hz). The simulated results are found to be in close agreement with the measured outcomes from the experiments. The proposed antenna proves to be promising for wearable MIMO applications.
Navigator‐based reacquisition and estimation of motion‐corrupted data: Application to multi‐echo spin echo for carotid wall MRI
Authors: Frost Robert , Biasiolli Luca , Li Linqing , Hurst Katherine , Alkhalil Mohammad , Choudhury Robin P. , Robson Matthew D. , Hess Aaron T. , Jezzard Peter 
Published: November 2019

PurposeTo assess whether artifacts in multi‐slice multi‐echo spin echo neck imaging, thought to be caused by brief motion events such as swallowing, can be corrected by reacquiring corrupted central k‐space data and estimating the remainder with parallel imaging.MethodsA single phase‐encode line (ky = 0, phase‐encode direction anteroposterior) navigator echo was used to identify motion‐corrupted data and guide the online reacquisition. If motion corruption was detected in the 7 central k‐space lines, they were replaced with reacquired data. Subsequently, GRAPPA reconstruction was trained on the updated central portion of k‐space and then used to estimate the remaining motion‐corrupted k‐space data from surrounding uncorrupted data. Similar compressed sensing‐based approaches have been used previously to compensate for respiration in cardiac imaging. The g‐factor noise amplification was calculated for the parallel imaging reconstruction of data acquired with a 10‐channel neck coil. The method was assessed in scans with 9 volunteers and 12 patients.ResultsThe g‐factor analysis showed that GRAPPA reconstruction of 2 adjacent motion‐corrupted lines causes high noise amplification; therefore, the number of 2‐line estimations should be limited. In volunteer scans, median ghosting reduction of 24% was achieved with 2 adjacent motion‐corrupted lines correction, and image quality was improved in 2 patient scans that had motion corruption close to the center of k‐space.ConclusionMotion‐corrupted echo‐trains can be identified with a navigator echo. Combined reacquisition and parallel imaging estimation reduced motion artifacts in multi‐slice MESE when there were brief motion events, especially when motion corruption was close to the center of k‐space.
The Pond Dye, Aquashade, Does Not Prevent Cyanobacterial Off-Flavors in Pond-Grown Channel Catfish
Authors: Tucker Craig S , Mischke Charles C 
Published: November 2019

Abstract
Aquashade is a dye that is used to control the growth of underwater weeds by reducing light penetration through water. We applied four, monthly applications of 1 μL/L Aquashade to three Channel Catfish Ictalurus punctatus ponds during the summer growing season to determine its effects on water quality and incidence of off-flavor in catfish. Three ponds were untreated controls. Dye treatments had no effect on nitrite or total ammonia concentrations, hours of supplemental aeration, phytoplankton standing crops, or fish production. Cyanobacteria that are known to produce odorous metabolites were found in all of the ponds at some time during the study. On the sampling date immediately before fish harvest, two ponds in each treatment contained populations of the odor-producing cyanobacterium, Planktothrix perornata and contained fish that would not be acceptable for processing because of musty off-flavors. The mechanism of action of the dye—reducing water-column penetration of light in wavelengths of ~600–650 nm—will have little effect on odor-producing species of cyanobacteria because those species have physiological adaptations (nonchlorophyll accessory pigments and the ability to regulate their position in the water column), which allow them to thrive in low-light environments.