bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age related extracellular matrix and interstitial cell phenotype in pulmonary valves
Authors: Wu Shaohua , Kumar Vikas , Xiao Peng , Kuss Mitchell , Lim Jung Yul , Guda Chittibabu , Butcher Jonathan , Duan Bin 
Published: December 2020

AbstractHeart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 months, denoted as young versus ~ 2 years, denoted as adult) and studied the effects of age on PV leaflet thickness, extracellular matrix components, and mechanical properties. We also conducted proteomics and RNA sequencing to investigate the global changes of PV leaflets and passage zero PV interstitial cells in their protein and gene levels. We found that the size, thickness, elastic modulus, and ultimate stress in both the radial and circumferential directions and the collagen of PV leaflets increased from young to adult age, while the ultimate strain and amount of glycosaminoglycans decreased when age increased. Young and adult PV had both similar and distinct protein and gene expression patterns that are related to their inherent physiological properties. These findings are important for us to better understand the physiological microenvironments of PV leaflet and valve cells for correctively engineering age-specific heart valve tissues.
Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma
Authors: Chang Kung-Chao , Chen Ruo-Yu , Wang Yu-Chu , Hung Liang-Yi , Medeiros L. Jeffrey , Chen Ya-Ping , Chen Tsai-Yun , Yang Jui-Chu , Chiang Po-Min 
Published: December 2020

AbstractDiffuse large B-cell lymphoma (DLBCL) may present initially inbone marrow,liver andspleen without any lymphadenopathy (referred to as BLS-type DLBCL), which is aggressive and frequently associated with hemophagocytic syndrome. Its tumorigenesis and molecular mechanisms warrant clarification. By gene microarray profiling with bioinformatics analysis, we found higher expression of the stem cell markers HOXA9 and NANOG, as well as BMP8B, CCR6 and S100A8 in BLS-type than conventional DLBCL. We further validated expression of these markers in a large cohort of DLBCL including BLS-type cases and found that expression of HOXA9 and NANOG correlated with inferior outcome and poor prognostic parameters. Functional studies with gene-overexpressed and gene-silenced DLBCL cell lines showed that expression of NANOG and HOXA9 promoted cell viability and inhibited apoptosis through suppression of G2 arrest in vitro and enhanced tumor formation and hepatosplenic infiltration in a tail-vein-injected mouse model. Additionally,HOXA9-transfected tumor cells showed significantly increased soft-agar clonogenic ability and tumor sphere formation. Interestingly, B cells with higher CCR6 expression revealed a higher chemotactic migration for CCL20. Taken together, our findings support the concept that tumor or precursor cells of BLS-type DLBCL are attracted by chemotaxis and home to the bone marrow, where the microenvironment promotes the expression of stem cell characteristics and aggressiveness of tumor cells.
Global phosphorus dynamics in terms of phosphine
Authors: Fu Wanyi , Zhang Xihui 
Published: December 2020

AbstractSince the detection of phosphine in the wastewater treatment plants in 1988, more and more investigations revealed that phosphine is closely related to ecological activities on a global scale. Here, we present perspectives on the whole dynamic cycles of phosphorus, particularly in terms of phosphine and its interactions with natural ecosystems, as well as the impacts from human activities. It may conclude that the phosphine-driving cycles of phosphorus depend on the coordination of human activities with natural ecosystems. Most importantly, the extensive recovery of phosphorus in numerous urban wastewater treatment plants may seriously obstruct its global cycles to catch up with the ecological needs in natural ecosystems. Phosphine gas plays an important role in the biogeochemical phosphorus cycle. Phosphorus might be one of the important elements participating in the global climate change together with carbon and nitrogen.
Reactivation during sleep with incomplete reminder cues rather than complete ones stabilizes long-term memory in humans
Authors: Forcato Cecilia , Klinzing Jens G. , Carbone Julia , Radloff Michael , Weber Frederik D. , Born Jan , Diekelmann Susanne 
Published: December 2020

AbstractReactivation by reminder cues labilizes memories during wakefulness, requiring reconsolidation to persist. In contrast, during sleep, cued reactivation seems to directly stabilize memories. In reconsolidation, incomplete reminders are more effective in reactivating memories than complete reminders by inducing a mismatch, i.e. a discrepancy between expected and actual events. Whether mismatch is likewise detected during sleep is unclear. Here we test whether cued reactivation during sleep is more effective for mismatch-inducing incomplete than complete reminders. We first establish that only incomplete but not complete reminders labilize memories during wakefulness. When complete or incomplete reminders are presented during 40-min sleep, both reminders are equally effective in stabilizing memories. However, when extending the retention interval for another 7 hours (following 40-min sleep), only incomplete but not complete reminders stabilize memories, regardless of the extension containing wakefulness or sleep. We propose that, during sleep, only incomplete reminders initiate long-term memory stabilization via mismatch detection.
Photonic-chip assisted correlative light and electron microscopy
Authors: Tinguely Jean-Claude , Steyer Anna Maria , Øie Cristina Ionica , Helle Øystein Ivar , Dullo Firehun Tsige , Olsen Randi , McCourt Peter , Schwab Yannick , Ahluwalia Balpreet Singh 
Published: December 2020

AbstractCorrelative light and electron microscopy (CLEM) unifies the versatility of light microscopy (LM) with the high resolution of electron microscopy (EM), allowing one to zoom into the complex organization of cells. Here, we introduce photonic chip assisted CLEM, enabling multi-modal total internal reflection fluorescence (TIRF) microscopy over large field of view and high precision localization of the target area of interest within EM. The photonic chips are used as a substrate to hold, to illuminate and to provide landmarking of the sample through specially designed grid-like numbering systems. Using this approach, we demonstrate its applicability for tracking the area of interest, imaging the three-dimensional (3D) structural organization of nano-sized morphological features on liver sinusoidal endothelial cells such as fenestrations (trans-cytoplasmic nanopores), and correlating specific endo-lysosomal compartments with its cargo protein upon endocytosis.
Development of a penem antibiotic against Mycobacteroides abscessus
Authors: Batchelder Hunter R. , Story-Roller Elizabeth , Lloyd Evan P. , Kaushik Amit , Bigelow Kristina M. , Maggioncalda Emily C. , Nuermberger Eric L. , Lamichhane Gyanu , Townsend Craig A. 
Published: December 2020

Abstractβ-lactams are the most widely used antibiotic class to treat bacterial infections in humans. Mycobacteroides abscessus is an emerging pulmonary pathogen resistant to most antibiotics, including penicillins and cephalosporins. With no current FDA-approved treatment and cure rates <50%, there is a pressing need for effective therapies. Here we report T405, a new β-lactam of the penem subclass that exhibits potent activity against M. abscessus and a panel of drug-resistant strains isolated from cystic fibrosis patients. Additionally, in combination with the β-lactamase inhibitor avibactam, the rate of spontaneous resistance of M. abscessus to T405 approached the limit of detection. Lastly, we show the favorable pharmacokinetic profile of T405 in mice and the absence of toxicity at elevated dosage, which support the clinical potential of this compound.
Imprinting and driving electronic orbital magnetism using magnons
Authors: Zhang Li-chuan , Go Dongwook , Hanke Jan-Philipp , Buhl Patrick M. , Grytsiuk Sergii , Blügel Stefan , Lux Fabian R. , Mokrousov Yuriy 
Published: December 2020

AbstractMagnons, as the most elementary excitations of magnetic materials, have recently emerged as a prominent tool in electrical and thermal manipulation and transport of spin, and magnonics as a field is considered as one of the pillars of modern spintronics. On the other hand, orbitronics, which exploits the orbital degree of freedom of electrons rather than their spin, emerges as a powerful platform in efficient design of currents and redistribution of angular momentum in structurally complex materials. Here, we uncover a way to bridge the worlds of magnonics and electronic orbital magnetism, which originates in the fundamental coupling of scalar spin chirality, inherent to magnons, to the orbital degree of freedom in solids. We show that this can result in efficient generation and transport of electronic orbital angular momentum by magnons, thus opening the road to combining the functionalities of magnonics and orbitronics to their mutual benefit in the realm of spintronics applications.
The origin of hysteresis and memory of two-phase flow in disordered media
Authors: Holtzman Ran , Dentz Marco , Planet Ramon , Ortín Jordi 
Published: December 2020

AbstractCyclic fluid-fluid displacements in disordered media feature hysteresis, multivaluedness, and memory properties in the pressure-saturation relationship. Quantitative understanding of the underlying pore-scale mechanisms and their extrapolation across scales constitutes a major challenge. Here we find that the capillary action of a single constriction in the fluid passage contains the key features of hysteresis. This insight forms the building block for an ab initio model that provides the quantitative link between the microscopic capillary physics, spatially-extended collective events (Haines jumps) and large-scale hysteresis. The mechanisms identified here apply to a broad range of problems in hydrology, geophysics and engineering.
Narrative and Other Pragmatic Language Abilities of Children With a History of Maltreatment
Authors: Ciolino Christel , Hyter Yvette D. , Suarez Michelle , Bedrosian Jan 
Published: December 2020

Introduction
In any given year, about 3.5 million children in the United States are suspected of having experienced maltreatment resulting in complex trauma involving the exposure to traumatic events and the long-term effects of this exposure on the child's psychological, emotional, social, linguistic, and communicative development.

Method
In this study, we analyzed retrospective data on the pragmatic language and social communication skills exhibited by children between the ages of 5 and 15 years with a documented history of maltreatment. Researchers analyzed the children's performance on the Hyter Pragmatic Protocol–Revised and the Clinical Evaluation of Language Fundamentals–Fifth Edition Screening Test to determine their syntactic, semantic, and pragmatic abilities. Analyses were also conducted to determine if children with different genders, ages, and numbers of exposures demonstrated differences in performance.

Results
Results showed that many children with trauma exposure demonstrated specific deficits in both narrative and expository language skills. High percentages of children exhibited difficulty using narrative cohesion, narrative coherence, expository content, expository structure, and expository coherence on the Hyter Pragmatic Protocol–Revised. The number of maltreatment exposures did not significantly impact performance on narrative measures. Female participants tended to perform better on pragmatic language tests. Younger children performed better than older children on narrative cohesion and produced a landscape of action in their narratives. Most children received passing scores on the Clinical Evaluation of Language Fundamentals–Fifth Edition Screening Test, and there were no significant differences in pass rates among age, gender, and number of exposures.

Conclusions
Clinical implications for assessment are discussed. Implications include the need for clinicians to test pragmatic language, especially the narrative language of children exposed to complex trauma. Because older children with trauma exposure demonstrated lower scores on certain tests of narrative language, there is a need for periodic reassessment of these pragmatic skills as children age.
Research on Economic Periodicity Based on Principal Component-Weighted Distance and Clustering Analysis
Authors: Yan Jiayi , Pu Qian , Liu Junfei 
Published: December 2020

Based on the knowledge of economics, this paper selects 22 macroeconomic indicators that best reflect the overall economic situation of the United States. After differential, logarithmic and exponential preprocessing of the original data, this paper, based on the power spectral analysis model, adaptively identifies the periodicity of the selected economic indicators, and visualize the results. As a result, it screens out 11 indicators with obvious periodicity. In the process of solving the weighted distance based on principal component analysis, correlation test is first conducted on the selected 11 single indicators of periodicity to obtain Pearson correlation heatmap. Then, the principal components are extracted by selecting the first five principal components as the virtual indicators to represent the monthly economic situation, and calculating the weighted distance value between months for visualization. Finally, we select the results of 36 months’ smoothing for analysis, figure out the time intervals with similar economic situation, and verify the conjecture of economic periodicity.
Finally, based on K-MEAN clustering analysis, the economic conditions of 352 months are classified into 3 clusters by using the weighted distance after 36 months’ smoothing. From the visualized results, it is found that there are two complete cycles, i.e. red-yellow-blue and red-yellow-blue, which is consistent with the conclusion of principal component analysis model, and proves the existence of economic cycle again.
In conclusion, based on the above PCA weighted distance and clustering analysis, it can be concluded that the economic period is around 176 months, in favor of medium long periodicity theory.
Fractured Neck of Femur Management at a District General Hospital: Adherence to NICE guidelines CG124 for total hip replacement
Authors: Mudiganty Srikanth , Kosmidis Ilias , Edwin John 
Published: December 2020

Introduction: The National Institute for Health and Care Excellence (NICE) in 2011 declared standards in the management of fracture neck of femur (NOF) patients suggesting a total hip replacement (THR) if necessary criteria were met. The Best Practice Tariff (BPT) states all NOF fracture patients should be operated on within 36 h of presentation to Accident & Emergency. We conducted this retrospective study for the years 2016–2018 to evaluate the adherence to these guidelines by Basildon and Thurrock University Hospital and compared the results with national standards. Methods: Data for the period from 2016 to 2018 was collected from the National Hip Fracture Database (NHFD) retrospectively. The data was analysed to calculate various procedures performed for fracture NOF fixations, the number of THR’s for displaced intracapsular fracture NOF, and percentage of patients operated within 36 h and evaluated reasons for the delay. Results: Over the 3 years, the number of THR eligible displaced intracapsular neck of femur fracture patients that underwent THR was above the national average. Across all 3 years, the number of patients who underwent surgery within 36 h was less than the national average. Administrative/logistic reasons for the delay were the major cause for delayed surgery in all 3 years. Conclusion: Compliance with the NICE guidelines and achievement of national standards in NOF fracture care is achievable by most district general hospitals. Awareness and implementation of NICE guidelines for THRs need to be enhanced. A sustained, continual team effort and strict vigilance are necessary to prevent delayed surgery.
Verdauungsoptimierung – wie sich Ernährung und Mikrobiom gegenseitig beeinflussen
Authors: Bischoff Stephan C. 
Published: December 2020

ZusammenfassungDie Nahrung beeinflusst die Darmbakterien dadurch, dass sie solche Bakterien zur Expansion stimuliert, welche die angebotenen Nahrungsbestandteile metabolisieren können. Umgekehrt helfen auf diese Weise die Darmbakterien, die Verdauung der Nahrung zu optimieren und die Energieaufnahme zu erhöhen. Somit wird das Darmmikrobiom vor allem durch die Ernährung reguliert, sie bestimmt die Zusammensetzung, die Funktion und die Diversität des Mikrobioms. In der Folge trägt das Darmmikrobiom dazu bei, die Energie- und Substratgewinnung aus der Nahrung zu optimieren. Dies ist bei drohender Mangelernährung von Vorteil, kann aber bei Nahrungsüberfluss und Adipositas nachteilig werden.
Rosmarinic Acid–Human Pharmacokinetics and Health Benefits
Authors: Hitl Maja , Kladar Nebojša , Gavarić Neda , Božin Biljana 
Published: December 2020

AbstractRosmarinic acid is a phenolic compound commonly found in the Lamiaceae (Labiateae) plant species. It is considered responsible for a wide spectrum of biological and pharmacological activities of plants containing this compound. The aim of the current review is to present the fate of rosmarinic acid inside the human body, explained through pharmacokinetic steps and to briefly present the health benefits of RA. Pharmacokinetics was at first studied in animal models, but several studies were conducted in humans as well. This compound can be applied topically, pulmonary, intranasally, and via intravenous infusion. However, peroral application is the main route of entry into the human body. Presumably, it is mainly metabolized by the gut microflora, providing simple, more easily absorbed phenolic units. Inside the body, the rosmarinic acid molecule undergoes structural changes, as well as conjugation reactions. Renal excretion represents the main path of elimination. Previously conducted studies reported no serious adverse effects of herbal remedies containing RA, as well as their positive effects on human health. In addition to in vitro studies, clinical investigations suggested its benefits in dermatological, allergic, and osteoarthritic disorders, as well as for improving cognitive performance and in metabolic syndrome treatment. Future studies should investigate the kinetics during long-term application in patients who would have potential benefits from RA usage. Pharmaceutical formulations designed to prevent the fast metabolism of RA and allow its penetration into other compartments of the human body are also interesting topics for future research.
Inhibition of Proinflammatory Cytokine Release by Flavones and Flavanones from the Leaves of Dracaena steudneri Engl.
Authors: Nchiozem-Ngnitedem Vaderament-A , Omosa Leonidah Kerubo , Bedane Kibrom Gebreheiwot , Derese Solomon , Spiteller Michael 
Published: December 2020

AbstractThe leaves of Dracaena steudneri yielded 6 new flavonoids–3,5,7-trihydroxy-6-methyl-3′,4′-methylenedioxyflavone (1), 5,7-dihydroxy-3-methoxy-6-methyl-3′,4′-methylenedioxyflavone (2), 3,5,7-trihydroxy-6-methoxy-3′,4′-methylenedioxyflavone (3), (2S,3S)-3,7-dihydroxy-6-methoxy-3′,4′-methylenedioxyflavanone (4), 4′,5,7-trihydroxy-3,3′,8-trimethoxy-6-methylflavone (5), (2R) 7-hydroxy-2′,8-dimethoxyflavanone (6)–together with 13 known congeners. Their structures were established using spectroscopic and spectrometric methods including NMR, CD, and HRMSn measurements. The compounds were evaluated for their anti-inflammatory potential through measurement of the levels of cytokines IL-1β, IL-2, GM-CSF, and TNF-α in the supernatant of human peripheral blood mononuclear cells stimulated by lipopolysaccharide. Flavones derivatives 1–4 with a C-3′/4′ methylenedioxy substituent led to a substantial increase in the production of IL-1β and GM-CSF out of 4 pro-inflammatory cytokines relative to LPS control. Quercetin derivatives 5, 11, and 13 with a hydroxyl group at C-4′ inhibited the production of IL-2, GM-CSF, and TNF-α. The presence of a C-2/C-3 double bond in 14 was pivotal to the significantly stronger (0.4 to 27.5% of LPS control) inhibitory effect compared to its dihydro derivative 8 (36.2 to 262.7% of LPS control) against all tested cytokines. It is important to note that the inhibitory activity of 14 was substantially higher than that of the standard drug used, ibuprofen.
Pathophysiology of Cerebral Edema—A Comprehensive Review
Authors: Dalby Tara , Wohl Elyana , Dinsmore Michael , Unger Zoe , Chowdhury Tumul , Venkatraghavan Lakshmikumar 
Published: December 2020

AbstractCerebral edema is a condition where an excess of cerebral water accumulates due to primary neurological or non-neurological causes. Cerebral edema complicates many brain pathologies causing additional injury often in excess of the original neurological insult. Classic descriptions divide cerebral edema into cytotoxic, vasogenic, interstitial, and osmotic subtypes. The interplay of different mechanisms is important in the clinical manifestations. Recent research has advanced our understanding of the molecular pathophysiology of cerebral edema, exposing the central role of aquaporins and specific ion channels. The aim of this review is to provide a comprehensive overview of the molecular pathophysiology of cerebral edema including unique disease specific mechanisms.