bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neoadjuvant systemic and hyperthermic intraperitoneal chemotherapy combined with cytoreductive surgery for gastric cancer patients with limited peritoneal metastasis: a prospective cohort study
Authors: Yu Pengfei , Ye Zeyao , Dai Gaiguo , Zhang Yanqiang , Huang Ling , Du Yian , Cheng Xiangdong 
Published: October 2020

Abstract
Background: There is no currently available treatment for peritoneal metastasis of gastric cancer. This phase II study aimed to evaluate the efficacy and safety of neoadjuvant systemic chemotherapy and hyperthermic intraperitoneal chemotherapy (HIPEC) combined with cytoreductive surgery (CRS) for the treatment of these patients.

Methods: Neoadjuvant chemotherapy comprised two cycles of HIPEC and four cycles of S-1 plus paclitaxel. HIPEC was administered intraperitoneally with paclitaxel (75 mg/m2). For systemic chemotherapy, paclitaxel was administered intravenously(150 mg/m2) on day 1，and S-1 was administered orally（80 mg/m2/day）on days 1-14 of a 3-week cycle. Another two cycles of HIPEC and four cycles of S-1 plus paclitaxel were administered after second diagnostic staging laparoscopy or CRS. The primary endpoint was treatment efficiency and safety; the secondary endpoint was 3-year overall survival (OS).

Results: A total of 40 patients were enrolled and 38 patients have been analyzed. Of these, 18 (47.4%) patients received neoadjuvant systemic chemotherapy, HIPEC and CRS (conversion therapy group), while 20 patients received only chemotherapy and HIPEC (palliative chemotherapy group). Median OS was markedly improved in the conversion therapy group (21.1 months, 95% confidence interval [CI] 16.7-25.6 months) in comparison with the palliative chemotherapy group(10.8 months, 95%CI 7.3-14.2 months, p=0.002). After neoadjuvant systemic chemotherapy and HIPEC, a second laparoscopic exploration was performed, and the prognosis of patients with low peritoneal cancer index (PCI) (PCI < 6) was significantly better than that of patients with high PCI (PCI≥6)(20.1 vs.11.3 months, p=0.006).

Conclusion: Neoadjuvant systemic chemotherapy and HIPEC combined with CRS was safe and feasible, and could potentially improve the prognosis of gastric cancer patients with limited peritoneal metastasis. However, further clinical trials are still warranted.
DNA methylation abnormalities of imprinted genes in congenital heart disease: A pilot study
Authors: Chang Shaoyan , Wang Yubo , Xin Yu , Wang Shuangxing , Luo Yi , Wang Li , Zhang Hui , Li Jia 
Published: October 2020

Abstract
Background: Congenital heart disease (CHD) is resulted from the interaction of genetic aberration and environmental factors. Imprinted genes, which are regulated by epigenetic modifications, are essential for the normal embryonic development. However, the role of imprinted genes in the pathogenesis of CHD remains unclear. Results: We investigated the effect of imprinted gene methylation on the pathogenesis of CHD. Eighteen imprinted genes that are known to affect early embryonic development were selected and the methylation modification genes were detected by massarray in 27 CHD children and 28 healthy children. Altered methylation of 8 imprinted genes was found, including 2 imprinted genes with hypermethylation of GRB10 and MEST and 6 genes with hypomethylation of PEG10 , NAP1L5 , INPP5F , PLAGL1 , NESP and MEG3 . Stratified analysis showed that the methylation degree of imprinted genes was different in different types of CHD. Risk analysis showed that 6 imprinted genes within a specific methylation level range were the risk factor for CHD except MEST and NAP1L5 . Conclusion: Altered methylation of imprinted genes is associated with CHD and varies in different types of CHD. Further experiments are warranted to identify the methylation characteristics of imprinted genes in different types of CHD and clarify the pathogenesis mechanism of imprinted genes in CHD.
Comparative Plastid Genomics of Four Pilea (Urticaceae) Species: Insight into Interspecific Plastid Genome Diversity in Pilea
Authors: Li Jingling , Tang Jianmin , Zeng Siyuan , Han Fang , Yuan Jing , Yu Jie 
Published: October 2020

Abstract
Background: Pilea is a genus of perennial herbs from the family Urticaceae, and some species are used as courtyard ornamentals or for medicinal purposes. At present, there is no information about the plastid genome of Pilea, which limits our understanding of this genus. Here, we report 4 plastid genomes of Pilea taxa (Pilea mollis Wedd., Pilea glauca 'Greizy', Pilea peperomioides Diels. and Pilea serpyllacea 'Globosa') and performed comprehensive comparative analysis.Results: The four plastid genomes all have a typical quartile structure. The lengths of the plastid genomes ranged from 150,398 bp to 152,327 bp, and each genome contained 113 unique genes, including 79 protein-coding genes, 4 rRNA genes, and 30 tRNA genes. Comparative analysis showed a rather high level of sequence divergence in the four genomes. Moreover, eight hypervariable regions were identified, petN-psbM, psbZ-trnG-GCC, trnT-UGU-trnL-UAA, accD-psbI, ndhF-rpl32, rpl32-trnL-UAG, ndhA-intron and ycf1, which are proposed for use as DNA barcode regions. Phylogenetic relationships based on the plastid genomes of 23 species of 14 genera of Urticaceae resulted in the placement of Pilea in the middle and lower part of the phylogenetic tree, with 100% bootstrap support within Urticaceae.Conclusion: Our results enrich the resources concerning plastid genomes. Comparative plastome analysis provides insight into the interspecific diversity of the plastid genome of Pilea. The identified hypervariable regions could be used for developing molecular markers applicable in various research areas.
Demand- and Supply-side Factors Associated with the Use of Contraceptive Methods in Pakistan: A Contemplative Study of Demographic and Health Surveys, 1990–2018
Authors: Jabeen Sadia , Rathor Adnan , Riaz Maria , Zakar Rubeena , Fischer Florian 
Published: October 2020

Abstract
Background: A remarkable decline in fertility rates has been observed in many countries, with a primary determinant being an increase in the use of contraceptives. However, the birth rate in Pakistan is still higher compared to the other countries of the region. Therefore, this study aims to assess the effect of demand- and supply-side factors associated with the use of contraceptive measures in Pakistan.Method: Secondary data analysis of four data series of the Pakistan Demographic and Health Surveys (PDHS 1990–91, 2006–07, 2012–13 and 2017–18) were used. The data includes ever-married women aged 15–49 years who had given birth in the previous five years and participated in the family planning module of the PDHS. A total of 25,318 women were included in the analysis. Data were analysed by investigating the associations between independent variables (demand- and supply-side factors) and the use of contraceptive measures through unadjusted odds ratios (OR) and adjusted OR (AOR).Results: The results among demand-side factors indicated that in 2012–13, women without media exposure were less likely to use contraceptives and the trend remains almost constant for 2017–18 (AOR = 0.664, 95 % CI: 0.562–0.784) in 2012–13 and (AOR = 0.654, 95 % CI: 0.483–0.885) in 2017–18; however, they still show a lower likelihood of using contraceptives without media exposure. The results among supply-side factors indicated that absence of transport (2012–13) and limited visits by family planning workers over the previous 12 months (2006–07, 2012–13 and 2017–18) remained significant factors for not using contraceptive methods. Conclusion: The results of the study indicate that certain demand- and supply-side factors are associated with the use of contraceptive measures in Pakistan. It highlights the need for the provision of family planning resources and further structural factors, particularly in remote areas.
A potential role for insulin treatment during pregnancy in reducing postpartum psychological distress in maternal obesity: An administrative population health study
Authors: Jarmasz Jessica Stawski , ANDERSON Alexandrea , Bock Margaret E. , Jin Yan , Cattini Peter A. , Ruth Chelsea 
Published: October 2020

Abstract
Background: Studies have found an association between obesity and an increased risk for peripartum depression, which has also been linked to decreased placental lactogen levels. In addition, women with obesity treated for gestational diabetes with insulin were found to have increased levels of placental lactogen. Treatment options exist for perinatal and postpartum depression however, they pose a risk to the developing offspring. Thus, prevention as well as markers for early identification of peripartum depression are needed. Therefore, our study objective is to identify the association between insulin treatment in pregnancy and the risk of postpartum psychological distress (abbreviated here as PPD) among cohorts of women with and without obesity.Methods: Administrative health data (2002/03-2018/19) were used to identify a cohort of women (age 15+ years) who gave birth (N=250,746) and had no pre-existing mood/anxiety disorders or diabetes (N=222,863 excluded). Women were then divided into two groups: lean (N=17,975) and with obesity (N=9,908), which was identified by a recorded maternal weight of >38 - <65.6 kg and ≥85 - <186 kg (respectively). The risk of PPD within one year after delivery with and without insulin treatment was assessed by Poisson regression analysis. Models were adjusted for maternal age group (at pregnancy start date) and area-level income (at delivery).Results: The unadjusted risk of PPD was higher in the obesity group (8.56%; 95% CI 8.00 - 9.15) than in the lean group (6.93%; 95% CI 6.56 - 7.33). When no insulin treatment was given during pregnancy, mothers with obesity had a significantly higher risk of PPD than the lean group (aRR 1.27; 95% CI 1.17-1.39; p<0.0001). However, when women with obesity and insulin treatment were compared to the lean group with no insulin treatment, no significant difference in the risk of PPD was observed between the groups (aRR 1.30; 95% CI 0.83-2.02; p=0.248).Conclusion: This is the first study to demonstrate a positive association between insulin treatment in pregnancy among women with obesity and reduced PPD rates, suggesting insulin as a preventative measure, and further supporting placental lactogen levels as a potential marker for early identification.
Surgical outcomes of the thoracic ossification of ligamentum flavum: A retrospective analysis of 61 cases
Authors: Yamada Tsuyoshi , Shindo Shigeo , Yoshii Toshitaka , Ushio Shuta , Kusano Kazuo , Miyake Norihiko , Arai Yoshiyasu , Otani Kazuyuki , Okawa Atsushi , Nakai Osamu 
Published: October 2020

Abstract
Background: Thoracic ossification of ligamentum flavum (T-OLF), as one of the causes of thoracic myelopathy, is often combined with other spinal disorders. Concurrent lumbar spinal canal stenosis (LCS) is often obscured by symptoms due to T-OLF, leading to difficulty in identifying the origin of these neurological findings. It is common to be misdiagnosed or delayed diagnosis due to the complicated nature. We evaluated the prevalence, distribution, and clinical characteristics of OLF, especially in patients with LCS.Methods: The authors performed a retrospective analysis of the outcomes of 61 patients who underwent thoracic surgeries performed for symptomatic T-OLF. In all the patients, whole spine lesions were evaluated preoperatively. We examined the factors related to poor outcomes (the recovery rate of the Japanese Orthopedic Association score for thoracic myelopathy is less than 40%) following OLF surgeries. We further compared the clinical outcomes according to whether there was concurrent LCS, and determined the optimal surgical approach.Results: The occurrence of T-OLF increased with age. Forty-six cases (75.4%) were considered to be tandem T-OLF and LCS (LCS group). An advanced age, and concurrent LCS were associated with a poor outcome after the surgery, rather than type of surgical procedure, the local T-OLF size, configuration, the number of OLF lesions, or radiographic cervical stenosis. The LCS group significantly included a greater number of elderly, and more light-weighted patients with Modic change in thoracic spine and a greater sagittal vertical axis, resulting in the lower neurological recovery. Additional lumbar surgery (13cases) effectively improved both the T-JOA and L-JOA scores (from 6.5 ± 2.0 points to 8.0 ± 1.8 points, p=0.0406, and from 14.5 ± 4.7 points to 20.7 ± 2.6 points, p=0.001, respectively) in OLF patients with LCS.Conclusions: T-OLF was highly associated with other spinal disorders. Poor outcomes in T-OLF surgery could be associated with age and concurrent LCS, and an additional surgery for another lumbar lesion significantly improved neurological findings in T-OLF patients.
Learning burnout: evaluating the role of social support in medical students
Authors: Zhang Jiayu , Shu Tao , Xiang Ming , Feng Zhanchun 
Published: October 2020

Abstract
Background:Burnout is a stress-induced syndrome that is considered closely related to work. Although social support could reduce burnout syndrome, the effect of it on learning burnout in medical students remains unclear. The objectives of the study are to evaluate the association between learning burnout and social support in a cohort of Chinese medical students.Methods:A cross-sectional online survey was distributed to students who participated in online learning in a medical college in Wuhan during the COVID-19 epidemic. We used the Lian version of the Maslach Burnout Inventory (MBI) to assess learning burnout and the Social Support Rating Scale (SSRS) to assess social support.Results:A total of 684 students completed the survey (response rate of 30.9%), of which 315 (46.12%) met standard criteria for learning burnout. We found grade, family income, learning time, and the number of interactions with teachers or classmates had an effect on students’ learning burnout. After adjusting for the grade and residence, there was a significant and relevant association between the social support and learning burnout. (adjusted odds ratio, 0.93 for 1-point decrease in total SSRS score; 95% CI, 0.90 – 0.96; p < 0.001).Conclusions:Learning burnout was highly prevalent in medical students of our college. The social support especially subjective support and utilization of support played a protective role in reducing the risk of learning burnout.
Socio-economic inequality in the prevalence of violence against older adults – Findings from India
Authors: Sinha Debashree , Mishra Prem Shankar , Srivast Shobhit , Kumar Pradeep 
Published: October 2020

Abstract
Background
Violence against older adults is a well-recognised socio-psychological and public health problem worldwide. It is uncared-for, undiagnosed, and untreated problem that is widespread across both developed and developing countries. Therefore, this paper aims to understand the extent of socio-economic status related inequality in violence against older adults.
Methods
Data for this study came from Building a Knowledge Base on Population Aging in India (BKPAI). Violence against older adults was the outcome variable for this study and defined as older adults who faced any abuse or violence or neglect or disrespect by any person. Bivariate analysis and regression-based decomposition technique is used to understand the relative contribution of various socioeconomic factors to violence among (N = 9541) older adults.
Results
The most dominant form of violence is verbal (10%) followed by disrespect (5.8%) and economic violence (5.2%). Older adults aged 80 + years [OR: 1.49; CI: 1.14–1.93] and working [OR: 1.26; CI: 1.02–1.56] had higher likelihood to suffer from violence than their counterparts. On the other hand older adults who were currently in union [OR: 0.79; CI: 0.65–0.95], living with children [OR: 0.53; CI: 0.40–0.72] and belonging to richer wealth quintile [OR: 0.35; CI: 0.24–0.51] had lower likelihood to suffer from violence than their counterparts. The decomposition results revealed that poor older adults are more prone to violence (Concentration index: -0.20). Household’s wealth status was responsible for 93.7% of the SES-related inequality whereas living arrangement of older adults explained 13.7% SES-related inequality. Education and working status of older adults made a substantial contribution to the inequalities in reported violence, explaining 3.7% and 3.3% of the total inequality, respectively.
Conclusion
With no or meagre income of their own, older adults belonging to the poorest wealth quintile have little or no bargaining power to secure a violent free environment for themselves. Therefore, special attention in terms of social and economic support should be given to the economically vulnerable older population.
Takotsubo Syndrome induced by brachytherapy in a patient with endocervical adenocarcinoma.
Authors: Vieira Aline Cristini , Ribeiro Mauricio Fernando Silva Almeida , Lima Julianne , Filho Jacob Sessim , Carvalho Heloisa de Andrade , Mano Max Senna 
Published: October 2020

Abstract
Background: Takotsubo syndrome (TTS), also known as stress cardiomyopathy, apical ballooning syndrome and broken heart syndrome, is characterized by acute-onset chest pain, electrocardiographic (ECG) abnormalities and reversible left ventricular (LV) disfunction in the absence of a culprit obstructive lesion in the coronary arteries; therefore, myocardial infarction is the most important differential diagnosis. Usually induced by emotional/physical stress, its treatment consists in hemodynamic support until complete and spontaneous recovery occurs, which is generally achieved within a few days to weeks. Cervical malignancies are an important public health issue in low/middle-income countries and, in the setting of locally advanced disease, concurrent chemoradiation followed by brachytherapy is considered the standard treatment, harboring curative potential. Case report: We report a case of a 38-year-old woman who underwent concurrent chemoradiotherapy and developed cardiopulmonary arrest in ventricular fibrillation during a brachytherapy session. Complementary tests disclosed altered ECG and cardiac biomarkers, no evidence of coronary artery obstruction, as well as LV disfunction consistent with TTS on echocardiogram and cardiac MRI. After few days of supportive therapy, complete recovery of heart function was observed. Conclusion: Especially for cancer patients, who usually experience intense emotional/physical stress intrinsically associated with their diagnosis and aggressive treatments, considering TTS as a differential diagnosis is warranted. Intracavitary brachytherapy procedure may represent a trigger for TTS.
Direct Influences of Mesospheric Gravity Waves on Ionosphere over Jammu &amp; Kashmir, India
Authors: Thokuluwa Ramkumar , Malik Manzoor , Ganaie Bilal , Bhat Aashiq 
Published: October 2020

Abstract
As a joint research collaboration between the National Atmospheric Research Laboratory (NARL), and the University of Kashmir (KU), NARL installed an all-sky airglow CCD imager (with centre wavelengths of 630nm, 557.7nm [2nm band widths] and 840nm [150 nm wide band with blocking notch at 866 nm to avoid the contamination of molecular oxygen emissions]) in the University campus in Srinagar (75E, 34N, geographic) Jammu and Kashmir, India (western Himalayan region). To understand the upper atmospheric dynamics and ionospheric electrodynamics and their associated physical coupling mechanisms, the imager observes airglow emissions of OH molecules and atomic and molecular oxygen occurring at the heights of ~80-300 km. First-time airglow observations in Kashmir commenced in the night of August 11, 2017 and the present work reports on the characteristics of first-time observation of Medium Scale Travelling Ionospheric Disturbances (MSTIDs with horizontal wavelengths of ~100-300 km) which occurred during 20:30 - 22:30 hr. IST (Indian standard time) on August 15, 2017 (India independence day). Initially, the phase front of MSTIDs was aligned along the north-west and south-east direction and moved at ~57 m/s towards the south-west direction and finally the westward direction by aligning along the meridian before they disappeared. Along with SAMI-3 ionospheric model simulations, simultaneous multiwavelength airglow observations indicate that secondary gravity waves generated due to dissipation of upward propagating mesospheric gravity waves in the heights of ~85-95 km would have contributed to the generation of MSTIDs in the F region ionospheric plasma through electrodynamical coupling between the E and F region (Perkins instability) ionosphere.
Non-enzymatic Stereoselective S-glycosylation of Polypeptides and Proteins
Authors: Niu Dawen , Wan Li-Qiang , Zhang Xia , Xu Shi-Yang , Shi Rong , Deng Li-Fan , Cao Jin-Ge , Zhou Li , Gong Yanqiu , Shu Xiaoling , Ren Haiyan , Dai Lunzhi , Qi Shiqian 
Published: October 2020

Abstract
Glycosylation (1-4) is an essential and powerful technique that Nature employs to regulate the properties and functions of proteins and polypeptides. Our capacity to emulate Nature’s power, however, is limited by the methods available (5) to perform glycosylation on these complex biomolecules. So far, very few glycosylation reactions could operate under the conditions tolerated by biomolecules (e.g., aqueous media, mild pH, and ambient temperature), and the need to install glycosyl groups in a stereo-controlled fashion poses additional, significant challenges. Here we report a non-enzymatic glycosylation reaction that builds axial S-glycosidic bonds under biorelevant conditions. Our strategy exploits the exceptional functional group tolerance of radical processes, and is enabled by the design and use of allyl glycosyl sulfones as precursors to glycosyl radicals. Our method could introduce a variety of glycosyl units to the cysteine residues of polypeptides in a highly selective fashion. The power of this method is further demonstrated in the direct glycosylation of bioexpressed proteins. Computational and experimental studies provide insights into the reaction mechanism.
Tyrosine and Drug-like Late-stage Benzylic Functionalization via Photoredox Catalysis
Authors: Brandhofer Tobias , Derdau Volker , Mendez María , Pöverlein Christoph , Mancheno Olga Garcia 
Published: October 2020

Abstract
Visible light mediated late-stage functionalization is a rising field in synthetic and medicinal chemistry, allowing the fast and diversified modification of valuable, potentially therapeutic compounds such as peptides. However, there are relatively few mild methodologies for the C(sp3)-H functionalization of complex peptides. Herein, we report a visible light mediated photocatalytic protocol for the benzylic C-H modification of tyrosine and related C-H bonds. The embraced radical-cation/deprotonation strategy enables an incorporation of a wide range of valuable functional groups in high yields and chemoselectivity. The mild reaction conditions, site-selectivity and high functional group tolerance was highlighted by the functionalization of complex peptides, drugs and natural products, providing a promising synthetic platform in medicinal chemistry.