bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disappearance of the shunt and lower cardiac index during exercise in small, unrepaired ventricular septal defects
Authors: Maagaard Marie , Eckerström Filip , Heiberg Johan , Asschenfeldt Benjamin , Ringgaard Steffen , Hjortdal Vibeke E. 
Published: March 2020

AbstractObjectives:Clinical studies have revealed decreased exercise capacity in adults with small, unrepaired ventricular septal defects. Increasing shunt ratio and growing incompetence of the aortic and pulmonary valve with retrograde flow during exercise have been proposed as reasons for the previously found reduced exercise parameters. With MRI, haemodynamic shunt properties were measured during exercise in ventricular septal defects.Methods:Patients with small, unrepaired ventricular septal defects and healthy peers were examined with MRI during exercise. Quantitative flow scans measured blood flow through ascending aorta and pulmonary artery. Scans were analysed post hoc where cardiac index, retrograde flows, and shunt ratio were determined.Results:In total, 32 patients (26 ± 6 years) and 28 controls (27 ± 5 years) were included. The shunt ratio was 1.2 ± 0.2 at rest and decreased to 1.0 ± 0.2 at peak exercise, p < 0.01. Aortic cardiac index was lower at peak exercise in patients (7.5 ± 2 L/minute/m2) compared with controls (9.0±2L l/minute/m2), p<0.01. Aortic and pulmonary retrograde flow was larger in patients during exercise, p < 0.01. Positive correlation was demonstrated between aortic cardiac index at peak exercise and previously established exercise capacity for all patients (r = 0.5, p < 0.01).Conclusions:Small, unrepaired ventricular septal defects revealed declining shunt ratio with increasing exercise and lower aortic cardiac index. Patients demonstrated larger retrograde flow both through the pulmonary artery and the aorta during exercise compared with controls. In conclusion, adults with unrepaired ventricular septal defects redistribute blood flow during exercise probably secondary to a more fixed pulmonary vascular resistance compared with age-matched peers.
A systematic review, and meta-analysis, examining the prevalence of price promotions on foods and whether they are more likely to be found on less-healthy foods
Authors: Kaur Asha , Lewis Thomas , Lipkova Veronika , Fernando Santhushya , Rayner Mike , Harrington Richard A , Waterlander Wilma , Scarborough Peter 
Published: March 2020

AbstractObjective:There are concerns that price promotions encourage unhealthy dietary choices. This review aims to answer the following research questions (RQ1) what is the prevalence of price promotions on foods in high-income settings, and (RQ2) are price promotions more likely to be found on unhealthy foods?Design:Systematic review of articles published in English, in peer-review journals, after 1 January 2000.Setting:Included studies measured the prevalence of price promotions (i.e. percentage of foods carrying a price promotion out of the total number of foods available to purchase) in retail settings, in upper-mid to high-income countries.Participants:‘Price promotion’ was defined as a consumer-facing temporary price reduction or discount available to all customers. The control group/comparator was the equivalent products without promotions. The primary outcome for this review was the prevalence of price promotions, and the secondary outcome was the difference between the proportions of price promotions on healthy and unhealthy foods.Results:Nine studies (239 344 observations) were included for the meta-analysis for RQ1, the prevalence of price promotions ranged from 6 % (95 % CI 2 %, 15 %) for energy-dense nutrient-poor foods to 15 % (95 % CI 9 %, 25 %) for cereals, grains, breads and other starchy carbohydrates. However, the I-squared statistic was 99 % suggesting a very high level of heterogeneity. Four studies were included for the analysis of RQ2, of which two supported the hypothesis that price promotions were more likely to be found on unhealthy foods.Conclusions:The prevalence of price promotions is very context specific, and any proposed regulations should be supported by studies conducted within the proposed setting(s).
Effect of crop canopy and herbicide application on kochia (Bassia scoparia) density and seed production
Authors: Mosqueda Elizabeth G. , Lim Charlemagne A. , Sbatella Gustavo M. , Jha Prashant , Lawrence Nevin C. , Kniss Andrew R. 
Published: March 2020

AbstractUnderstanding the effects of crop management practices on weed survival and seed production is imperative in improving long-term weed management strategies, especially for herbicide-resistant weed populations. Kochia [Bassia scoparia (L.) A.J. Scott] is an economically important weed in western North American cropping systems for many reasons, including prolific seed production and evolved resistance to numerous herbicide sites of action. Field studies were conducted in 2014 in a total of four field sites in Wyoming, Montana, and Nebraska to quantify the impact of different crop canopies and herbicide applications on B. scoparia density and seed production. Crops used in this study were spring wheat (Triticum aestivum L.), dry bean (Phaseolus vulgaris L.), sugar beet (Beta vulgaris L.), and corn (Zea mays L.). Herbicide treatments included either acetolactate synthase (ALS) inhibitors effective on non-resistant B. scoparia or a non–ALS inhibiting herbicide effective for both ALS-resistant and ALS-susceptible B. scoparia. Bassia scoparia density midseason was affected more by herbicide choice than by crop canopy, whereas B. scoparia seed production per plant was affected more by crop canopy compared with herbicide treatment. Our results suggest that crop canopy and herbicide treatments were both influential on B. scoparia seed production per unit area, which is likely a key indicator of long-term management success for this annual weed species. The lowest germinable seed production per unit area was observed in spring wheat treated with non–ALS inhibiting herbicides, and the greatest germinable seed production was observed in sugar beet treated with ALS-inhibiting herbicides. The combined effects of crop canopy and herbicide treatment can minimize B. scoparia establishment and seed production.
Identifying Metasomatized Continental Lithospheric Mantle Involvement in Cenozoic Magmatism From Ta/Th Values, Southwestern North America
Authors: Farmer G. Lang , Fritz Diane E. , Glazner Allen F. 
Published: March 2020

AbstractThe accessory minerals rutile and apatite are rare or absent in the convecting upper mantle but occur in shallow, cooler, metasomatized continental lithospheric mantle (CLM) where they serve as carrier phases for the trace elements Ta (in rutile) and Th (in apatite). Because both minerals crystallize near‐solidus and are eliminated early during partial mantle melting, the relative abundances of rutile and apatite should control the Ta and Th abundances of mantle melts and provide a means of identifying the involvement of rutile‐ and/or apatite‐bearing metasomatized CLM in mafic continental magmatism. As a test, we investigated published Ta and Th abundances data from ~2,000 whole‐rock samples of mafic to intermediate composition, Cenozoic volcanic rocks in southwestern North America. Roughly half of the samples have Ta/Th values similar to those of island arc volcanic rocks (<0.2) or ocean island and mid‐ocean ridge basalts (>0.6). The remaining samples have intermediate and variable Ta/Th values between 0.2 and 0.6, independent of specific indices of crustal interaction (e.g., wt% P2O5/wt% K2O). We interpret the intermediate Ta/Th rocks as the products of direct melting of, or of extensive melt‐rock interaction with, rutile‐ and/or apatite‐bearing CLM. Intermediate Ta/Th rocks also have uniformly high 87Sr/86Sr (0.706 to 0.708) compared to oceanic basalts that, unlike their Nd isotopic compositions, do not covary with lithospheric age. These observations are consistent with widespread metasomatism of the CLM by Sr‐rich, Nd‐poor, aqueous fluids generated by dehydration of oceanic lithosphere, and its overlying tectonic mélange during early Cenozoic subduction beneath southwestern North America.
Absolute Centroid Location of Submarine Earthquakes from 3D Waveform Modeling of Water Reverberations
Authors: Castellanos Jorge C. , Zhan Zhongwen , Wu Wenbo 
Published: March 2020

AbstractOceanic transform faults (OTFs) represent an attractive tectonic environment to investigate how slip is accommodated within the crust. However, as most of these fault systems grow in the deep ocean, where few local seismic observations are available, characterizing their earthquake behavior is complicated and remains a formidable challenge. Here we present a novel approach for retrieving precise centroid locations of submarine earthquakes that is based on the modeling of water phases in teleseismic records. Using a hybrid method for simulating far‐field body waves with 3D source‐side structures, we demonstrate that the scattered energy generated by the continuous bounces of an earthquake's P‐wave trapped in the ocean is modelable and carries information about its source location. As a case study, we use a realistic bathymetry model of the Gofar transform fault on the East Pacific Rise and simulate the seismic wavefield at US Array stations for four of its moderate‐sized (Mw5.0+) earthquakes. Our modeling results show that water phases are sensitive to a ∼5 km change in the earthquake's horizontal location and that a remarkable agreement between observed and synthetic water phases is achieved when the location of an event is close to its true one. We then relocate three of these events by systematically computing their water phases in candidate locations until a satisfactory waveform fit is achieved. This analysis technique paves a new route for studying earthquake source properties in isolated marine environments and serves as a mean to investigate the seismic behavior of OTFs on a global scale.
Global Response of Evapotranspiration Ratio to Climate Conditions and Watershed Characteristics in a Changing Environment
Authors: Liu Zhiyong , Cheng Linyin , Zhou Guoyi , Chen Xiaohong , Lin Kairong , Zhang Wenfeng , Chen Xiuzhi , Zhou Ping 
Published: March 2020

AbstractUnderstanding the complex relationship between evapotranspiration and climate conditions and land surface characteristics is important within the context of global warming and increasing anthropogenic stressors. Based on the Budyko theory, we derived general frameworks for describing how the evapotranspiration ratio (actual evapotranspiration/precipitation, AET/P) responds to changes in the aridity index (potential evapotranspiration/precipitation, PET/P) and watershed characteristics (m). The frameworks show that the sensitivity of AET/P to PET/P decreases with increasing aridity but such decreasing pattern also depends on different watershed characteristics. The sensitivity coefficient tends to be greater in the energy‐limited regimes (above 0.3) than in the water‐limited regimes. The slope of the sensitivity curve becomes steeper for larger m values (e.g., m > 2). In addition, the sensitivity of the AET/P to m shows an asymmetrical unimodal curve, while the strongest sensitivity (inflection point) of evapotranspiration ratio to m occurs at PET/P > 1. We further examined spatial patterns of these sensitivities and the relative roles of PET/P (RCPET/P) and m (RCm) to AET/P over 91 hydrological basins worldwide. The tropical humid basins present the strongest sensitivity of AET/P to PET/P, while higher sensitivity coefficients of AET/P to m can be found in very cold basins. The changes in the sensitivity of evapotranspiration ratio to PET/P and m between 1930–1970 and 1971–2008 were assessed for these basins across broad climatic gradients. Most of these basins show a positive change in RCPET/P but a negative shift in RCm in the warming world.
Nuclear DLC1 exerts oncogenic function through association with FOXK1 for cooperative activation of MMP9 expression in melanoma
Authors: Yang Xintao , Hu Feng , Liu Jessica Aijia , Yu Shan , Cheung May Pui Lai , Liu Xuelai , Ng Irene Oi-Lin , Guan Xin-Yuan , Wong Kelvin K. W. , Sharma Rakesh , Lung Hong Lok , Jiao Yufei , Lee Leo Tsz On , Cheung Martin 
Published: March 2020

AbstractA Rho GTPase-activating protein (RhoGAP), deleted in liver cancer 1 (DLC1), is known to function as a tumor suppressor in various cancer types; however, whether DLC1 is a tumor-suppressor gene or an oncogene in melanoma remains to be clarified. Here we revealed that high DLC1 expression was detected in most of the melanoma tissues where it was localized in both the nuclei and the cytoplasm. Functional studies unveiled that DLC1 was both required and sufficient for melanoma growth and metastasis. These tumorigenic events were mediated by nuclear-localized DLC1 in a RhoGAP-independent manner. Mechanistically, mass spectrometry analysis identified a DLC1-associated protein, FOXK1 transcription factor, which mediated oncogenic events in melanoma by translocating and retaining DLC1 into the nucleus. RNA-sequencing profiling studies further revealed MMP9 as a direct target of FOXK1 through DLC1-regulated promoter occupancy for cooperative activation of MMP9 expression to promote melanoma invasion and metastasis. Concerted action of DLC1–FOXK1 in MMP9 gene regulation was further supported by their highly correlated expression in melanoma patients’ samples and cell lines. Together, our results not only unravel a mechanism by which nuclear DLC1 functions as an oncogene in melanoma but also suggest an unexpected role of RhoGAP protein in transcriptional regulation.
Regulation of the small GTPase Ran by miR-802 modulates proliferation and metastasis in colorectal cancer cells
Authors: Wang Xin , Li Danxiu , Sun Lina , Shen Gaofei , Liu Hao , Guo Hao , Ge Minghui , Liang Junrong , Chen Ping , Zhou Jinchi , Cao Tianyu , Wang Qi , Gao Xiaoliang , Tong Mingfu , Hu Sijun , Nie Yongzhan , Fan Daiming , wang xin , Zhao Xiaodi , Lu Yuanyuan 
Published: March 2020

Abstract
Background
The small GTPase Ran is upregulated in multiple cancers and fundamental for cancer cell survival and progression, but its significance and molecular mechanisms in colorectal cancer (CRC) remain elusive.

Methods
Ran expression was detected in CRC cell lines and tumour tissues. In vitro and in vivo functional assays were performed to examine the effects of Ran on cell proliferation and metastasis. The pathways and effectors regulated by Ran were explored by an unbiased screening. Bioinformatics prediction and experimental validation were used to identify the miRNA regulator for Ran.

Results
Ran expression was frequently increased in metastatic CRC cells and tissues, especially in metastatic tissues. The upregulation of Ran correlated with poor CRC patient prognosis. Ran silencing reduced proliferation and metastasis of CRC cells both in vitro and in vivo. Ran regulated the expression of EGFR and activation of ERK and AKT signalling pathways. miR-802 was identified as an upstream regulator of Ran and miR-802 overexpression resulted in antiproliferative and antimetastatic activities.

Conclusion
Our study demonstrates the oncogenic roles and underlying mechanisms of Ran in CRC and the novel miR-802/Ran/EGFR regulatory axis may provide potential biomarkers for the treatment of CRC.
Mapping anthropogenic mineral generation in China and its implications for a circular economy
Authors: Zeng Xianlai , Ali Saleem H. , Tian Jinping , Li Jinhui 
Published: March 2020

AbstractAnthropogenic mineral is absorbing wide concern in the context of circular economy, but its generation mechanism and quantity from product to waste remain unclear. Here we consider three product groups, 30 products, and use the revised Weibull lifespan model to map the generation of anthropogenic mineral and 23 types of the capsulated materials by targeting their evolution from 2010 to 2050. Total weight of anthropogenic mineral on average in China reached 39 Mt in 2010, but it will double in 2022 and quadruple in 2045. Stocks of precious metals and rare earths will increase faster than most base materials. The total economic potential in yearly-generated anthropogenic mineral is anticipated to grow markedly from 100 billion US$ in 2020 to 400 billion US$ in 2050. Furthermore, anthropogenic mineral of around 20 materials will be capable to meet projected consumption of three product groups by 2050.
Microglial metabolic flexibility supports immune surveillance of the brain parenchyma
Authors: Bernier Louis-Philippe , York Elisa M. , Kamyabi Alireza , Choi Hyun B. , Weilinger Nicholas L. , MacVicar Brian A. 
Published: March 2020

AbstractMicroglia are highly motile cells that continuously monitor the brain environment and respond to damage-associated cues. While glucose is the main energy substrate used by neurons in the brain, the nutrients metabolized by microglia to support surveillance of the parenchyma remain unexplored. Here, we use fluorescence lifetime imaging of intracellular NAD(P)H and time-lapse two-photon imaging of microglial dynamics in vivo and in situ, to show unique aspects of the microglial metabolic signature in the brain. Microglia are metabolically flexible and can rapidly adapt to consume glutamine as an alternative metabolic fuel in the absence of glucose. During insulin-induced hypoglycemia in vivo or in aglycemia in acute brain slices, glutaminolysis supports the maintenance of microglial process motility and damage-sensing functions. This metabolic shift sustains mitochondrial metabolism and requires mTOR-dependent signaling. This remarkable plasticity allows microglia to maintain their critical surveillance and phagocytic roles, even after brain neuroenergetic homeostasis is compromised.
CDK1 dependent phosphorylation of hTERT contributes to cancer progression
Authors: Yasukawa Mami , Ando Yoshinari , Yamashita Taro , Matsuda Yoko , Shoji Shisako , Morioka Masaki Suimye , Kawaji Hideya , Shiozawa Kumiko , Machitani Mitsuhiro , Abe Takaya , Yamada Shinji , Kaneko Mika K. , Kato Yukinari , Furuta Yasuhide , Kondo Tadashi , Shirouzu Mikako , Hayashizaki Yoshihide , Kaneko Shuichi , Masutomi Kenkichi 
Published: March 2020

AbstractThe telomerase reverse transcriptase is upregulated in the majority of human cancers and contributes directly to cell transformation. Here we report that hTERT is phosphorylated at threonine 249 during mitosis by the serine/threonine kinase CDK1. Clinicopathological analyses reveal that phosphorylation of hTERT at threonine 249 occurs more frequently in aggressive cancers. Using CRISPR/Cas9 genome editing, we introduce substitution mutations at threonine 249 in the endogenous hTERT locus and find that phosphorylation of threonine 249 is necessary for hTERT-mediated RNA dependent RNA polymerase (RdRP) activity but dispensable for reverse transcriptase and terminal transferase activities. Cap Analysis of Gene Expression (CAGE) demonstrates that hTERT phosphorylation at 249 regulates the expression of specific genes that are necessary for cancer cell proliferation and tumor formation. These observations indicate that phosphorylation at threonine 249 regulates hTERT RdRP and contributes to cancer progression in a telomere independent manner.
A common MET polymorphism harnesses HER2 signaling to drive aggressive squamous cell carcinoma
Authors: Kong Li Ren , Mohamed Salleh Nur Afiqah Binte , Ong Richard Weijie , Tan Tuan Zea , Syn Nicholas L. , Goh Robby Miguel , Fhu Chee Wai , Tan Daniel S. W. , Iyer N. Gopalakrishna , Kannan Srinivasaraghavan , Verma Chandra S. , Lim Yaw Chyn , Soo Ross , Ho Jingshan , Huang Yiqing , Lim Joline S. J. , Yan Benedict Junrong , Nga Min En , Lim Seng Gee , Koeffler H. Phillip , Lee Soo Chin , Kappei Dennis , Hung Huynh The , Goh Boon Cher 
Published: March 2020

Abstractc-MET receptors are activated in cancers through genomic events like tyrosine kinase domain mutations, juxtamembrane splicing mutation and amplified copy numbers, which can be inhibited by c-MET small molecule inhibitors. Here, we discover that the most common polymorphism known to affect MET gene (N375S), involving the semaphorin domain, confers exquisite binding affinity for HER2 and enables METN375S to interact with HER2 in a ligand-independent fashion. The resultant METN375S/HER2 dimer transduces potent proliferative, pro-invasive and pro-metastatic cues through the HER2 signaling axis to drive aggressive squamous cell carcinomas of the head and neck (HNSCC) and lung (LUSC), and is associated with poor prognosis. Accordingly, HER2 blockers, but not c-MET inhibitors, are paradoxically effective at restraining in vivo and in vitro models expressing METN375S. These results establish METN375S as a biologically distinct and clinically actionable molecular subset of SCCs that are uniquely amenable to HER2 blocking therapies.
Generating high quality libraries for DIA MS with empirically corrected peptide predictions
Authors: Searle Brian C. , Swearingen Kristian E. , Barnes Christopher A. , Schmidt Tobias , Gessulat Siegfried , Küster Bernhard , Wilhelm Mathias 
Published: March 2020

AbstractData-independent acquisition approaches typically rely on experiment-specific spectrum libraries, requiring offline fractionation and tens to hundreds of injections. We demonstrate a library generation workflow that leverages fragmentation and retention time prediction to build libraries containing every peptide in a proteome, and then refines those libraries with empirical data. Our method specifically enables rapid, experiment-specific library generation for non-model organisms, which we demonstrate using the malaria parasite Plasmodium falciparum, and non-canonical databases, which we show by detecting missense variants in HeLa.
Sustainability of global Golden Inland Waterways
Authors: Wang Yichu , Chen Xiabin , Borthwick Alistair G. L. , Li Tianhong , Liu Huaihan , Yang Shengfa , Zheng Chunmiao , Xu Jianhua , Ni Jinren 
Published: March 2020

AbstractSustainable inland waterways should meet the needs of navigation without compromising the health of riverine ecosystems. Here we propose a hierarchical model to describe sustainable development of the Golden Inland Waterways (GIWs) which are characterized by great bearing capacity and transport need. Based on datasets from 66 large rivers (basin area > 100,000 km2) worldwide, we identify 34 GIWs, mostly distributed in Asia, Europe, North America, and South America, typically following a three-stage development path from the initial, through to the developing and on to the developed stage. For most GIWs, the exploitation ratio, defined as the ratio of actual to idealized bearing capacity, should be less than 80% due to ecological considerations. Combined with the indices of regional development, GIWs exploitation, and riverine ecosystem, we reveal the global diversity and evolution of GIWs’ sustainability from 2015 to 2050, which highlights the importance of river-specific strategies for waterway exploitation worldwide.
A reflection on lithium-ion battery cathode chemistry
Authors: Manthiram Arumugam 
Published: March 2020

AbstractLithium-ion batteries have aided the portable electronics revolution for nearly three decades. They are now enabling vehicle electrification and beginning to enter the utility industry. The emergence and dominance of lithium-ion batteries are due to their higher energy density compared to other rechargeable battery systems, enabled by the design and development of high-energy density electrode materials. Basic science research, involving solid-state chemistry and physics, has been at the center of this endeavor, particularly during the 1970s and 1980s. With the award of the 2019 Nobel Prize in Chemistry to the development of lithium-ion batteries, it is enlightening to look back at the evolution of the cathode chemistry that made the modern lithium-ion technology feasible. This review article provides a reflection on how fundamental studies have facilitated the discovery, optimization, and rational design of three major categories of oxide cathodes for lithium-ion batteries, and a personal perspective on the future of this important area.