bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adenosine Triphosphate Neutralizes Pneumolysin-Induced Neutrophil Activation
Authors: Cuypers Fabian , Klabunde Björn , Gesell Salazar Manuela , Surabhi Surabhi , Skorka Sebastian B , Burchhardt Gerhard , Michalik Stephan , Thiele Thomas , Rohde Manfred , Völker Uwe , Hammerschmidt Sven , Siemens Nikolai 
Published: May 2020

Abstract

Background
In tissue infections, adenosine triphosphate (ATP) is released into extracellular space and contributes to purinergic chemotaxis. Neutrophils are important players in bacterial clearance and are recruited to the site of tissue infections. Pneumococcal infections can lead to uncontrolled hyperinflammation of the tissue along with substantial tissue damage through excessive neutrophil activation and uncontrolled granule release. We aimed to investigate the role of ATP in neutrophil response to pneumococcal infections.

Methods
Primary human neutrophils were exposed to the pneumococcal strain TIGR4 and its pneumolysin-deficient mutant or directly to different concentrations of recombinant pneumolysin. Neutrophil activation was assessed by measurement of secreted azurophilic granule protein resistin and profiling of the secretome, using mass spectrometry.

Results
Pneumococci are potent inducers of neutrophil degranulation. Pneumolysin was identified as a major trigger of neutrophil activation. This process is partially lysis independent and inhibited by ATP. Pneumolysin and ATP interact with each other in the extracellular space leading to reduced neutrophil activation. Proteome analyses of the neutrophil secretome confirmed that ATP inhibits pneumolysin-dependent neutrophil activation.

Conclusions
Our findings suggest that despite its cytolytic activity, pneumolysin serves as a potent neutrophil activating factor. Extracellular ATP mitigates pneumolysin-induced neutrophil activation.
What is the most appropriate metric for the assessment of volume of antibiotic use?
Authors: Vlahović-Palčevski Vera , Pulcini Céline 
Published: May 2020

Abstract
One of the core elements of antibiotic stewardship is surveillance and monitoring of quantity of antibiotic use. This requires tools for measuring the quantity of antibiotic use. However, these metrics have not been standardized, and different metrics are used across different countries, regions and individual healthcare settings. In the literature, there is much controversy on the most appropriate metric for monitoring antibiotic use. Several authors have questioned the relevance of DDDs, which are recommended by the WHO, while others still prefer using DDDs as the best available metric. The results of the recent DRIVE-AB project suggest that a combination of metrics may be the best approach, since all metrics have limitations and provide different perspectives.
Addressing the Implementation Crisis: Securing Reparation and Righting Wrongs
Authors: Murray Rachel 
Published: May 2020

AbstractThere has been increasing attention to the implementation of decisions of human rights bodies by scholars and by supranational institutions, states, litigants, and civil society. A project funded by the Economic and Social Research Council (ESRC) tracked the implementation by nine states of decisions adopted by human rights courts and commissions in the European, African and Inter-American systems and by select UN treaty bodies. This article summarizes the methodology and findings of the Project and in so doing forms an introduction to a series of articles and practice notes published in this special issue. A range of factors are identified from the research which influence implementation and stress the importance of a multifaceted, multidimensional approach to the issues. Implementation is not automatic and requires mechanisms, processes, and the involvement of actors (national and supranational) for states to comply with the reparations ordered in the decision. A case-by-case, state-by-state, context-specific approach is needed, tailored to the circumstances. This has implications for the manner in which litigants present their submissions, engage with state and supranational bodies and for the latter in terms of their roles and relationships with the various actors.
Balancing Specificity of Reparation Measures and States’ Discretion to Enhance Implementation
Authors: Murray Rachel , Sandoval Clara 
Published: May 2020

AbstractA recurrent statement when implementation of international orders or recommendations in individual cases is considered is the belief that greater specificity of the measures helps compliance. Our research project examined a number of decisions adopted by some of the UN treaty bodies, and the regional human rights commissions and courts, in Africa, the Americas and Europe, and attempted to trace the extent to which the reparations ordered by the supranational bodies were implemented by the state authorities. This article focuses primarily on the reparations ordered by the Inter-American, African and UN systems and attempts to define specificity, emphasizing that it refers to a constant process of refining and clarifying the meaning of different forms of reparation. Specificity is then ‘unpacked’ in terms of the content of the reparation, deadlines imposed, who is responsible and who is a victim, and how the decision is reasoned. In so doing, the article maintains that specificity must also be considered vis-à-vis the degree of discretion that is given (or not) to states to act on orders or recommendations given by supranational bodies in individual cases. We conclude that a more nuanced approach to specificity versus ambiguity is needed, tailored to each reparation, each state and each case.
Wedge resection as an alternative treatment for octogenarian and older patients with early-stage non–small-cell lung cancer
Authors: Mimae Takahiro , Miyata Yoshihiro , Tsutani Yasuhiro , Imai Kentaro , Ito Hiroyuki , Nakayama Haruhiko , Ikeda Norihiko , Okada Morihito 
Published: May 2020

Abstract

Objective
Anatomical resection with lymph node dissection is the standard treatment for early non–small cell lung cancer, whereas wedge resection is considered as a compromise. We aimed to determine whether wedge resection without lymph node dissection could be a treatment option for patients aged ≥80 years.

Methods
We assessed 669 patients with clinical stage IA non–small cell lung cancer, whole tumour sizes ≤2 cm and a consolidation to tumour ratio of &gt;0.5 who underwent R0 resection at three institutions between 2010 and 2016. We selected 58 of them who were aged ≥80 years and analysed their clinicopathological findings and prognosis after surgical procedures over a median follow-up of 38 months. Propensity scores for surgical procedures were calculated using age, gender, smoking status and solid tumour size on computed tomography.

Results
Three-year overall and recurrence-free survival rates after wedge resection and after segmentectomy + lobectomy for patients aged ≥80 years did not significantly differ (overall survival: 88.9% [95% confidence interval, 69.4–96.3%] vs. 75.5% [95% confidence interval, 51.5–88.8%], P = 0.95; recurrence-free survival: 85.2% [95% confidence interval: 65.2–94.2%] vs. 68.0% [95% confidence interval, 44.4–83.2], P = 0.57). Multivariable Cox regression analysis of overall survival with propensity scores revealed that surgical procedure was not an independent predictor of a poor prognosis (hazard ratio: 0.86 (0.28–2.6), P = 0.78) in patients aged ≥80 years.

Conclusions
Wedge resection might be an alternative to lobectomy or segmentectomy with lymph node dissection for patients aged ≥80 years with early non–small cell lung cancer.
Prominent genetic structure across native and introduced ranges of Pluchea indica, a mangrove associate, as revealed by microsatellite markers
Authors: Lin Yuting , Banerjee Achyut Kumar , Wu Haidan , Tan Fengxiao , Feng Hui , Tan Guangwen , Guo Wuxia , Huang Yelin 
Published: May 2020

AbstractAimsPluchea indica is a mangrove-associate species, known for its medicinal properties in its native range and being invasive in part of its introduced range. This study aimed to assess geographic distribution of genetic variation of this species across its distribution range, identify the factors influencing its genetic structure and use this information to suggest conservation and management strategies in its native and introduced ranges, respectively.MethodsWe assessed the genetic diversity and population structure of 348 individuals from 31 populations across its native (Asia) and introduced (USA) ranges for 15 nuclear microsatellite loci. The spatial pattern of genetic variation was investigated at both large and regional spatial scales with the hypothesis that geographic distance and natural geographic barriers would influence the population structure with varying levels of differentiation across spatial scales.Important FindingsWe found relatively high genetic diversity at the population level and pronounced genetic differentiation in P. indica, as compared with the genetic diversity parameters of mangroves and mangrove associates in this region. Most of the populations showed heterozygote deficiency, primarily due to inbreeding and impediment of gene flow. Analysis of population structures at large spatial scale revealed the presence of two major clusters across the species’ natural range separating populations in China from those in Indonesia, Malaysia, Singapore, Thailand, Cambodia and Philippines, and that the USA population might have been introduced from the population cluster in China. Genetic differentiation between populations was also observed at the regional scale. A large number of populations showed evidence of genetic bottleneck, thereby emphasizing the risk of local extinction. Based on these findings, our study recommends in situ conservation strategies, such as to prioritize populations for conservation actions and to maintain genetic diversity.
Emotional Echoes: Young People, Divorce, and the Public Media, 1960–2000
Authors: Vallgårda Karen , Larsen Katrine Rønsig 
Published: May 2020

Abstract
The article introduces the methodological concept of emotional echoing to analyze the cultural formation of individual emotions and demonstrates its utility in an analysis of young people’s emotional experience of parental divorce in Denmark between 1960 and 2000. Drawing on recent theorizations within the history of emotions, we posit that emotional echoing is a key element in the historical processes through which emotions are configured. Individuals more or less consciously employ collective templates of emotionality to interpret and convey their own feelings, giving shape to experience and subjectivity in the process. We then present a study of a variety of Danish public media to examine how young people interpreted and described the experience of parental divorce. We argue that a new public emotionality emerged at this time and that this impacted young people’s experiences. Growing divorce rates, the breakdown of patriarchal family structures, the rising status of children and youth, and the blooming media market all helped spur this change. Echoing each other’s tropes, practices, and images, young people emphasized the difficult and painful aspects of the emotional experience of divorce. During the last decades of the century, they increasingly articulated anger toward their parents, but they also more often emphasized “bad conscience” and signaled the importance of regulating one’s own emotions to protect one’s parents. Through nonmechanical and creative echoing, young people processed their emotions by digesting and contributing anonymously to the public media.
Dimethylarsinic acid is the causal agent inducing rice straighthead disease
Authors: Tang Zhong , Wang Yijie , Gao Axiang , Ji Yuchen , Yang Baoyun , Wang Peng , Tang Zhu , Zhao Fang-Jie 
Published: May 2020

Abstract
Straighthead disease is a physiological disorder in rice with symptoms of sterile spikelets, distorted husks, and erect panicles. Methylated arsenic species have been implicated as the causal agent of the disease, but direct evidence is lacking. Here, we investigated whether dimethylarsinic acid (DMA) causes straighthead disease and its effect on the transcriptome of young panicles. DMA addition caused typical straighthead symptoms in hydroponic culture, which were alleviated by silicon addition. DMA addition to soil at the tillering to flowering stages induced straighthead disease. Transgenic rice expressing a bacterial arsenite methyltransferase gene gained the ability to methylate arsenic to mainly DMA, with the consequence of inducing straighthead disease. Field surveys showed that seed setting rate decreased with increasing DMA concentration in the husk, with an EC50 of 0.18 mg kg−1. Transcriptomic analysis showed that 364 and 856 genes were significantly up- and down-regulated, respectively, in the young panicles of DMA-treated plants compared with control, whereas Si addition markedly reduced the number of genes affected. Among the differentially expressed genes, genes related to cell wall modification and oxidative stress responses were the most prominent, suggesting that cell wall metabolism is a sensitive target of DMA toxicity and silicon protects against this toxicity.
Spontaneous Ovarian Hyperstimulation Syndrome in a Partial Molar Pregnancy With Early Onset Severe Pre-eclampsia at 15 Weeks Gestation
Authors: Davies Michael J , Fletcher Tara L , Cox Bauer Callie M 
Published: May 2020

Abstract
Ovarian hyperstimulation syndrome is a well-known entity in assisted reproductive technology. However, it is unusual for this entity to occur without any medications that stimulate follicle stimulating hormone. Herein, we describe a case where a partial molar pregnancy with high human chorionic gonadotropin promiscuously activated follicle stimulating hormone receptors has resulted in spontaneous ovarian hyperstimulation syndrome. There are only eight other cases documented per our literature search of ovarian hyperstimulation syndrome in molar gestations, and this is the third report in partial molar gestation. In our case, it is an unique development of severe early onset pre-eclampsia in the second trimester. Our goal is to discuss the nuances in management of this entity as well as to add the available body of research on this subject.
Annual Disease Experience by Type and Correlations with Unmet Healthcare Needs among ROK Military Personnel
Authors: Kim Hwi Jun , Oh Sarah So Yeon , Choi Dong Woo , Won Sun Yeong , Kim Hae Jung , Ko Sung Chan , Jang Sung-In , Park Eun-Cheol 
Published: May 2020

Abstract

Introduction
The National Statistical Yearbook of Defense 2018 issued by the Republic of Korea (ROK) Ministry of National Defense reported that the number of patients using military hospitals steadily increased from 2008 to 2017. However, in the outpatient clinic statistics for years 2015–2017 from the ROK Armed Forces Medical Command, the amount of medical care received from some medical departments, such as the infection medicine, surgery, and anesthesiology departments, decreased. Therefore, the purpose of this study was to observe the differences in incidence of military personnel’s unmet healthcare needs according to number of diseases by type.

Materials and Methods
The study used data from the Military Health Survey, which was conducted from 2014 to 2015 and included 5162 responses from ROK military personnel. The number of diseases by type and unmet healthcare needs were self-reported. A multiple logistic regression analysis was used to examine the validity of the annual disease experience by type and correlations with unmet healthcare needs.

Results
Of the 5162 military personnel, 25.2% experienced unmet healthcare needs, and the more people with the number of disease by type, the more likely they were to experience unmet healthcare needs (1: 13.4%, 2: 22.9%, 3: 29.2%, 4: 34.5%, 5: 41.4%). The logistic regression analysis also revealed significant differences (1 = REF, 2 odds ratio (OR) = 1.83, 95% confidence interval (CI): 1.50–2.24; 3 OR = 2.53, 95% CI: 2.05–3.11, 4 OR = 3.10, 95% CI = 2.49–3.85; ≥5 OR = 3.85, 95% CI = 3.08–4.81). In addition, subgroup analysis showed that female military personnel are more likely to experience unmet healthcare needs than are male military personnel. We have also confirmed that working areas and private insurance can affect unmet healthcare needs.

Conclusion
This study suggests that unmet healthcare needs are influenced by the number of disease by the type of ROK military personnel. It is therefore necessary to strive to reduce the number of military personnel who experience unmet healthcare needs through this data.
Novel antifungal activity of oligostyrylbenzenes compounds on Candida tropicalis biofilms
Authors: Quinteros Melisa A , Galera Ivana L D , Tolosa Juan , García-Martínez Joaquín C , Páez Paulina L , Paraje María G 
Published: May 2020

Abstract

As sessile cells of fungal biofilms are at least 500-fold more resistant to antifungal drugs than their planktonic counterparts, there is a requirement for new antifungal agents. Olygostyrylbenzenes (OSBs) are the first generation of poly(phenylene)vinylene dendrimers with a gram-positive antibacterial activity. Thus, this study aimed to investigate the antifungal activity of four OSBs (1, 2, 3, and 4) on planktonic cells and biofilms of Candida tropicalis. The minimum inhibitory concentration (MIC) for the planktonic population and the sessile minimum inhibitory concentrations (SMIC) were determined. Biofilm eradication was studied by crystal violet stain and light microscopy (LM), and confocal laser scanning microscopy (CLSM) was also utilized in conjunction with the image analysis software COMSTAT. Although all the OSBs studied had antifungal activity, the cationic OSBs were more effective than the anionic ones. A significant reduction of biofilms was observed at MIC and supraMIC50 (50 times higher than MIC) for compound 2, and at supraMIC50 with compound 3. Alterations in surface topography and the three-dimensional architecture of the biofilms were evident with LM and CLSM. The LM analysis revealed that the C. tropicalis strain produced a striking biofilm with oval blastospores, pseudohyphae, and true hyphae. CLSM images showed that a decrease occurred in the thickness of the mature biofilms treated with the OSBs at the most effective concentration for each one. The results obtained by microscopy were supported by those of the COMSTAT program. Our results revealed an antibiofilm activity, with compound 2 being a potential candidate for the treatment of C. tropicalis infections.

Lay Summary
This study aimed to investigate the antifungal activity of four OSBs (1, 2, 3, and 4) on planktonic cells and biofilms of Candida tropicalis. Our results revealed an antibiofilm activity, with compound 2 being a potential candidate for the treatment of C. tropicalis infections.
Pulsed disc accretion driven by Hot jupiters
Authors: Teyssandier Jean , Lai Dong 
Published: May 2020

ABSTRACT
We present 2D hydrodynamical simulations of hot Jupiters orbiting near the inner edge of protoplanetary discs. We systemically explore how the accretion rate at the inner disc edge is regulated by a giant planet of different mass, orbital separation, and eccentricity. We find that a massive (with planet-to-star mass ratio ≳0.003) eccentric (ep ≳ 0.1) planet drives a pulsed accretion at the inner edge of the disc, modulated at one or two times the planet’s orbital frequency. The amplitude of accretion variability generally increases with the planet mass and eccentricity, although some non-monotonic dependences are also possible. Applying our simulation results to the T Tauri system CI Tau, where a young hot Jupiter candidate has been detected, we show that the observed luminosity variability in this system can be explained by pulsed accretion driven by an eccentric giant planet.
Dust reverberation mapping of type 2 AGN NGC 2110 realized with X-ray and 3–5 μm IR monitoring
Authors: Noda Hirofumi , Kawamuro Taiki , Kokubo Mitsuru , Minezaki Takeo 
Published: May 2020

ABSTRACT
The dust reverberation mapping is one of powerful methods to investigate the structure of the dusty tori in active galactic nuclei (AGNs), and it has been performed on more than a hundred type 1 AGNs. However, no clear results have been reported on type 2 AGNs because their strong optical/UV extinction completely hides their accretion disc emission. Here, we focus on an X-ray-bright type 2 AGN, NGC 2110, and utilize 2–20 keV X-ray variation monitored by MAXI to trace disc emission, instead of optical/UV variation. Comparing it with light curves in the WISE infrared (IR) W1 band ($\lambda =3.4~\mu$m) and W2 band ($\lambda =4.6~\mu$m) with cross-correlation analyses, we found candidates of the dust reverberation time lag at ∼60, ∼130, and ∼1250 d between the X-ray flux variation and those of the IR bands. By examining the best-fitting X-ray and IR light curves with the derived time lags, we found that the time lag of ∼130 d is most favoured. With this time lag, the relation between the time lag and luminosity of NGC 2110 is consistent with those in type 1 AGNs, suggesting that the dust reverberation in NGC 2110 mainly originates in hot dust in the torus innermost region, the same as in type 1 AGNs. As demonstrated by the present study, X-ray and IR simultaneous monitoring can be a promising tool to perform the dust reverberation mapping on type 2 AGNs.
White-light solar corona structure observed by naked eye and processed images
Authors: Rušin Vojtech , Prikryl Paul , Prikryl Emil A 
Published: May 2020

ABSTRACT
Light and dark adaptation and luminance contrast enhancement are well-known characteristics of human vision that allow us to observe a wide range of light intensity not fully captured in standard camera images. The naked-eye observations of total eclipses, some recorded with spectacular detail in artists’ paintings, reveal structure that is consistent with images obtained by telescopes equipped with recording media. The actual shape of the corona during a total eclipse depends not only on the phase of the solar cycle but, as can be simply demonstrated, also on the day-to-day variability and spatial distribution of coronal intensity that is determined by solar surface magnetic fields, including the locations of coronal holes that are the sources of high-speed solar wind causing geomagnetic storms. The latter were very similar for the eclipses in 1932, 1994, and 2017, which is the main reason why the naked-eye observations, as well as the processed images (1994 and 2017), of the white-light corona displayed very similar shapes. White-light corona image processing is a useful technique to enhance the contrast to observe fine-scale structure that is consistent with the physics of the solar atmosphere shaped by the magnetic field drawn out into the interplanetary space by solar wind.
Bar rejuvenation in S0 galaxies?
Authors: Barway Sudhanshu , Saha Kanak 
Published: May 2020

ABSTRACT
Based on the colour measurements from a multiband, multicomponent 2D decompositions of S0 and spiral galaxies using SDSS images, we found that bars are bluer in S0 galaxies compared to the spiral galaxies. Most of the S0s in our sample have stellar masses ∼L* galaxies. The environment might have played an important role as most of the S0s with bluer bars are in the intermediate-density environment. The possibility of minor mergers and tidal interactions that occurs frequently in the intermediate-density environment might have caused either a bar to form and/or induce star formation in the barred region of S0 galaxies. The underlying discs show the usual behaviour being redder in S0s compared to spiral galaxies while the bulges are red and old for both S0 and spiral galaxies. The finding of bluer bars in S0 galaxies is a puzzling issue and poses an interesting question at numerical and theoretical studies most of which shows that the bars are long-lived structures with old stellar populations.