bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid Cellulose Acetate Electrophoresis
Authors: Bartlett Raymond C 
Published: January 2020

Abstract
A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and γ-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has afforded excellent quantitations of serum protein fractions.
A Simple and Rapid Method for the Paper Electrophoretic Determination of Urinary Proteins
Authors: Collens Richard , Meyers Harry , Lange Kurt 
Published: January 2020

Abstract
A method is described for the simple, rapid, and accurate determination of paper electrophoretic patterns of urinary proteins using polyethylene glycol (Carbowax 20 M) for the concentration of the urine. The electrophoretic patterns of sera with normal and abnormal protein constituents did not change when added to a protein-free urine and subjected to the method described. The electrophoretic determination of urinary proteins as a routine procedure may give an insight into the physical type of permeability disturbance in the kidneys of a patient.
The Stability of Glucose in Serum
Authors: Ruiter Joseph , Weinberg Frederick , Morrison Andrew 
Published: January 2020

Abstract
The destruction of glucose does not occur in separated serum during the 48 hr. immediately following collection.
If necessary, the determination of the glucose level of serum can be delayed at least 48 hr. without introducing a significant error, provided the serum is removed from the clot immediately after collection.
Protein-Bound Iodine, Total Iodine, and Butanol-extractable Iodine by Partial Automation
Authors: Benotti Joseph , Benotti Norbert 
Published: January 2020

Abstract
The determination of iodine compounds in serum is simplified by using an automatic system to record the decolorization of the ceric sulfate solution. A method for modifying existing technics for PBI and BEI determinations, prior to this step, is described. Good agreement between the automated and manual technics was achieved.
Improvements on the Automatic Determination of Micro Amounts of Serum Calcium
Authors: Malmstadt H V , Hadjiioannou T P 
Published: January 2020

Abstract
Calcium in 0.2 ml. of blood serum can be titrated automatically in about 10 sec. with a reproducibility of 1-2%. It is not necessary to deproteinize the serum, and the complete procedure requires less than 1 min.
Hemoglobin at High Altitude as Related to Age
Authors: Dill D B , Terman J W , Hall F G 
Published: January 2020

Abstract
During the summer of 1962 the early phase of acclimatization to high altitude was studied in 6 of those who participated in the international high-altitude expedition to Chile in 1935. Ages of the 6 ranged from 58 to 71. Two also had taken part in a high-altitude study in 1929. In their earlier years these subjects had had an increase in hemoglobin concentration beginning with their arrival at high altitude. This response has been well established, especially by the Pike's Peak party led by Haldane and the work of Hurtado and associates in Peru. On the other hand, 5 of the 6 in the 1962 party exhibited a decrease in hemoglobin concentration during the first few days. The greatest decrease was observed in the oldest subject. His hemoglobin was 88% of his sea level value after 9 days at altitude and remained below his sea level value for another week. No observations were made on blood volume; hence, we can only speculate regarding possible related changes.
High-Temperature Operation of Oxygen Electrode Giving Fast Response for Respiratory Gas Sampling
Authors: Severinghaus John W 
Published: January 2020

Abstract
The 98% response time of the membrane-covered Clark-type polarographic oxygen electrode may be reduced to 0.4 sec. by heating the electrode and the gas sample flowing past it to 80° when the electrode is covered with 0.25-mil (6 µ) Teflon membrane. The design of the microcathode electrode has been modified slightly to prevent drying out of the electrolyte and pressure changes within the electrolyte cavity. The relationship of output current to temperature approaches a plateau at this temperature which suggests that for reasonably accurate operation, temperature regulation may not be as crucial as it is at body temperature.
Genetic lineage tracing reveals poor angiogenic potential of cardiac endothelial cells
Authors: Kocijan Tea , Rehman Michael , Colliva Andrea , Groppa Elena , Leban Matteo , Vodret Simone , Volf Nina , Zucca Gabriele , Cappelletto Ambra , Piperno Giulia Maria , Zentilin Lorena , Giacca Mauro , Benvenuti Federica , Zhou Bin , Adams Ralf H , Zacchigna Serena 
Published: January 2020

Abstract

Aims
Cardiac ischaemia does not elicit an efficient angiogenic response. Indeed, lack of surgical revascularization upon myocardial infarction results in cardiomyocyte death, scarring, and loss of contractile function. Clinical trials aimed at inducing therapeutic revascularization through the delivery of pro-angiogenic molecules after cardiac ischaemia have invariably failed, suggesting that endothelial cells in the heart cannot mount an efficient angiogenic response. To understand why the heart is a poorly angiogenic environment, here we compare the angiogenic response of the cardiac and skeletal muscle using a lineage tracing approach to genetically label sprouting endothelial cells.

Methods and results
We observed that overexpression of the vascular endothelial growth factor in the skeletal muscle potently stimulated angiogenesis, resulting in the formation of a massive number of new capillaries and arterioles. In contrast, response to the same dose of the same factor in the heart was blunted and consisted in a modest increase in the number of new arterioles. By using Apelin-CreER mice to genetically label sprouting endothelial cells we observed that different pro-angiogenic stimuli activated Apelin expression in both muscle types to a similar extent, however, only in the skeletal muscle, these cells were able to sprout, form elongated vascular tubes activating Notch signalling, and became incorporated into arteries. In the heart, Apelin-positive cells transiently persisted and failed to give rise to new vessels. When we implanted cancer cells in different organs, the abortive angiogenic response in the heart resulted in a reduced expansion of the tumour mass.

Conclusion
Our genetic lineage tracing indicates that cardiac endothelial cells activate Apelin expression in response to pro-angiogenic stimuli but, different from those of the skeletal muscle, fail to proliferate and form mature and structured vessels. The poor angiogenic potential of the heart is associated with reduced tumour angiogenesis and growth of cancer cells.
MMP inhibitors attenuate doxorubicin cardiotoxicity by preventing intracellular and extracellular matrix remodelling
Authors: Chan Brandon Y H , Roczkowsky Andrej , Cho Woo Jung , Poirier Mathieu , Sergi Consolato , Keschrumrus Vic , Churko Jared M , Granzier Henk , Schulz Richard 
Published: January 2020

Abstract

Aims
Heart failure is a major complication in cancer treatment due to the cardiotoxic effects of anticancer drugs, especially from the anthracyclines such as doxorubicin (DXR). DXR enhances oxidative stress and stimulates matrix metalloproteinase-2 (MMP-2) in cardiomyocytes. We investigated whether MMP inhibitors protect against DXR cardiotoxicity given the role of MMP-2 in proteolyzing sarcomeric proteins in the heart and remodelling the extracellular matrix.

Methods and results
Eight-week-old male C57BL/6J mice were treated with DXR weekly with or without MMP inhibitors doxycycline or ONO-4817 by daily oral gavage for 4 weeks. Echocardiography was used to determine cardiac function and left ventricular remodelling before and after treatment. MMP inhibitors ameliorated DXR-induced systolic and diastolic dysfunction by reducing the loss in left ventricular ejection fraction, fractional shortening, and E′/A′. MMP inhibitors attenuated adverse left ventricular remodelling, reduced cardiomyocyte dropout, and prevented myocardial fibrosis. DXR increased myocardial MMP-2 activity in part also by upregulating N-terminal truncated MMP-2. Immunogold transmission electron microscopy showed that DXR elevated MMP-2 levels within the sarcomere and mitochondria which were associated with myofilament lysis, mitochondrial degeneration, and T-tubule distention. DXR-induced myofilament lysis was associated with increased titin proteolysis in the heart which was prevented by ONO-4817. DXR also increased the level and activity of MMP-2 in human embryonic stem cell-derived cardiomyocytes, which was reduced by ONO-4817.

Conclusions
MMP-2 activation is an early event in DXR cardiotoxicity and contributes to myofilament lysis by proteolyzing cardiac titin. Two orally available MMP inhibitors ameliorated DXR cardiotoxicity by attenuating intracellular and extracellular matrix remodelling, suggesting their use may be a potential prophylactic strategy to prevent heart injury during chemotherapy.
Incorporation of a unified protein abundance dataset into the Saccharomyces genome database
Authors: Nash Robert S , Weng Shuai , Karra Kalpana , Wong Edith D , Engel Stacia R , Cherry J Michael ,   SGD Project
Published: January 2020

Abstract
The identification and accurate quantitation of protein abundance has been a major objective of proteomics research. Abundance studies have the potential to provide users with data that can be used to gain a deeper understanding of protein function and regulation and can also help identify cellular pathways and modules that operate under various environmental stress conditions. One of the central missions of the Saccharomyces Genome Database (SGD; https://www.yeastgenome.org) is to work with researchers to identify and incorporate datasets of interest to the wider scientific community, thereby enabling hypothesis-driven research. A large number of studies have detailed efforts to generate proteome-wide abundance data, but deeper analyses of these data have been hampered by the inability to compare results between studies. Recently, a unified protein abundance dataset was generated through the evaluation of more than 20 abundance datasets, which were normalized and converted to common measurement units, in this case molecules per cell. We have incorporated these normalized protein abundance data and associated metadata into the SGD database, as well as the SGD YeastMine data warehouse, resulting in the addition of 56 487 values for untreated cells grown in either rich or defined media and 28 335 values for cells treated with environmental stressors. Abundance data for protein-coding genes are displayed in a sortable, filterable table on Protein pages, available through Locus Summary pages. A median abundance value was incorporated, and a median absolute deviation was calculated for each protein-coding gene and incorporated into SGD. These values are displayed in the Protein section of the Locus Summary page. The inclusion of these data has enhanced the quality and quantity of protein experimental information presented at SGD and provides opportunities for researchers to access and utilize the data to further their research.
Labour Market Shocks and the Demand for Trade Protection: Evidence from Online Surveys
Authors: Di Tella Rafael , Rodrik Dani 
Published: January 2020

AbstractWe study preferences for government action in response to layoffs resulting from different types of labour-market shocks. We consider: technological change, a demand shift, bad management and three kinds of international outsourcing. Support for government intervention rises sharply in response to shocks and is heavily biased towards trade protection. Trade shocks generate more demand for protectionism and, among trade shocks, outsourcing to a developing country elicits greater demand for protectionism. The ‘bad management’ shock is the only scenario that induces a desired increase in compensatory transfers. Trump supporters are more protectionist than Clinton supporters, but preferences seem easy to manipulate: Clinton supporters primed with trade shocks are as protectionist as baseline Trump voters. Highlighting labour abuses in the exporting country increases the demand for trade protection by Clinton supporters but not Trump supporters.
Long-term outcomes of upfront surgery in patients with resectable pathological N2 non-small-cell lung cancer
Authors: Yun Jae Kwang , Bok Jin San , Lee Geun Dong , Kim Hyeong Ryul , Kim Yong-Hee , Kim Dong Kwan , Park Seung-Il , Choi Sehoon 
Published: January 2020

Abstract

OBJECTIVES
Although the standard treatment for pathological N2 (pN2) non-small-cell lung cancer (NSCLC) patients is definitive chemoradiation, surgery can be beneficial for resectable pN2 disease. Herein, we report the long-term clinical outcomes of upfront surgery followed by adjuvant treatment for selected patients with resectable pN2 disease.

METHODS
We performed a retrospective analysis of clinical outcomes for patients with pN2 disease who underwent surgery as the first-line therapy. Multivariable Cox regression analysis was used to identify the significant factors for overall survival (OS) and recurrence-free survival.

RESULTS
From 2004 to 2015, a total of 706 patients with pN2 NSCLC underwent complete anatomical resection at our institution. The patients’ clinical N stages were cN0, 308 (43.6%); cN1, 123 (17.4%) and cN2, 275 (39.0%). Adjuvant chemotherapy, radiotherapy and chemoradiotherapy were administered to 169 (23.9%), 115 (17.4%) and 299 patients (42.4%), respectively. With a median follow-up of 40 months, the respective median time and 5-year rate of OS were 52 months and 44.7%. According to subdivided pN2 descriptors, the median OS time was 80, 53 and 37 months for patients with pN2a1, pN2a2 and pN2b, respectively. Adjuvant chemotherapy was a significant prognostic factor for both OS [hazard ratio (HR) 0.39, 95% confidence interval (CI) 0.28–0.52; P &lt; 0.001] and recurrence-free survival (HR 0.42, 95% CI 0.30–0.58; P &lt; 0.001).

CONCLUSIONS
Upfront surgery followed by adjuvant therapy for resectable N2 disease showed favourable outcomes compared to those reported in previous studies. Adjuvant chemotherapy is essential to improve the prognosis for patients undergoing upfront surgery for N2 disease.
Which Countries Send More Delegates to Climate Change Conferences? Analysis of UNFCCC COPs, 1995–2015
Authors: Kaya Ayse , Schofield Lynne Steuerle 
Published: January 2020

Abstract
The size of national delegations at the most critical intergovernmental climate change conferences—the annual gatherings of the Conference of the Parties (COPs) of the United Nations Framework Convention on Climate Change—vary greatly. The literature has emphasized the importance of national delegation size (NDS) for states’ formal and informal participation in climate change negotiations. To our knowledge, however, this is the first paper to comprehensively examine the determinants of NDS from 1995–2015. The findings highlight a country's resources and its interest in the mitigation of fossil fuel emissions as important determinants of its NDS. In contrast, the evidence for a connection between vulnerability to climate change and NDS is limited. Interest group politics appear more important than civil society or bureaucratic influence in determining NDS. In terms of policy implications, the distance between the country and the COP location is a robust deterrent of larger delegations, and there is a nonlinear relationship between NDS and financial capacity. Further, there are differences across Annex I and non-Annex I countries.
BetaScan2: Standardized Statistics to Detect Balancing Selection Utilizing Substitution Data
Authors: Siewert Katherine M , Voight Benjamin F 
Published: January 2020

AbstractLong-term balancing selection results in a build-up of alleles at similar frequencies and a deficit of substitutions when compared with an outgroup at a locus. The previously published β(1) statistics detect balancing selection using only polymorphism data. We now propose the β(2) statistic which detects balancing selection using both polymorphism and substitution data. In addition, we derive the variance of all β statistics, allowing for their standardization and thereby reducing the influence of parameters which can confound other selection tests. The standardized β statistics outperform existing summary statistics in simulations, indicating β is a well-powered and widely applicable approach for detecting balancing selection. We apply the β(2) statistic to 1000 Genomes data and report two missense mutations with high β scores in the ACSBG2 gene. An implementation of all β statistics and their standardization are available in the BetaScan2 software package at https://github.com/ksiewert/BetaScan.
Complete Genome Sequencing of FourArcobacterSpecies Reveals a Diverse Suite of Mobile Elements
Authors: Miller William G , Yee Emma , Bono James L 
Published: January 2020

AbstractArcobacter species are recovered from a wide variety of sources, including animals, food, and both fresh and marine waters. Several Arcobacter species have also been recovered from human clinical samples and are thus associated tentatively with food- and water-borne human illnesses. Genome sequencing of the poultry isolate Arcobacter cibarius H743 and the Arcobacter acticola, Arcobacter pacificus, and Arcobacter porcinus type strains identified a large number and variety of insertion sequences. This study presents an analysis of these A. acticola, A. cibarius, A. pacificus, and A. porcinus IS elements. The four genomes sequenced here contain 276 complete and degenerate IS elements, representing 13 of the current 29 prokaryotic IS element families. Expansion of the analysis to include 15 other previously sequenced Arcobacter spp. added 73 complete and degenerate IS elements. Several of these IS elements were identified in two or more Arcobacter species, suggesting movement by horizontal gene transfer between the arcobacters. These IS elements are putatively associated with intragenomic deletions and inversions, and tentative movement of antimicrobial resistance genes. The A. cibarius strain H743 megaplasmid contains multiple IS elements common to the chromosome and, unusually, a complete ribosomal RNA locus, indicating that larger scale genomic rearrangements, potentially resulting from IS element-mediated megaplasmid cointegration and resolution may be occurring within A. cibarius and possibly other arcobacters. The presence of such a large and varied suite of mobile elements could have profound effects on Arcobacter biology and evolution.
Patients’ and Caregivers’ Attitudes Toward Deprescribing in Singapore
Authors: Kua Chong-Han , Reeve Emily , Tan Doreen S Y , Koh Tsingyi , Soong Jie Lin , Sim Marvin J L , Zhang Tracy Y , Chen Yi Rong , Ratnasingam Vanassa , Mak Vivienne S L , Lee Shaun Wen Huey ,   Pharmaceutical Society of Singapore Deprescribing Workgroup
Published: January 2020

Abstract

Background
Knowledge of decision-making preference of patients and caregivers is needed to facilitate deprescribing. This study aimed to assess the perspectives of caregivers and older adults towards deprescribing in an Asian population. Secondary objectives were to identify and compare characteristics associated with these attitudes and beliefs.

Method
A cross-sectional survey of two groups of participants was conducted using the Revised Patients’ Attitudes Towards Deprescribing questionnaire. Descriptive results were reported for participants’ characteristics and questionnaire responses from four factors (belief in medication inappropriateness, medication burden, concerns about stopping, and involvement) and two global questions. Correlation between participant characteristics and their responses was analyzed.

Results
A total of 1,057 (615 older adults; 442 caregivers) participants were recruited from 10 institutions in Singapore. In which 511 (83.0%) older adults and 385 (87.1%) caregivers reported that they would be willing to stop one or more of their medications if their doctor said it was possible, especially among older adults recruited from acute-care hospitals (85.3%) compared with older adults in community pharmacies (73.6%). Individuals who take more than five medications and those with higher education were correlated with greater agreement in inappropriateness and involvement, respectively.

Conclusions
Clinicians should consider discussing deprescribing with older adults and caregivers in their regular clinical practice, especially when polypharmacy is present. Further research is needed into how to engage older adults and caregivers in shared decision making based on their attitudes toward deprescribing.
A mouse model for kinesin family member 11 (Kif11)-associated familial exudative vitreoretinopathy
Authors: Wang Yanshu , Smallwood Philip M , Williams John , Nathans Jeremy 
Published: January 2020

Abstract
During mitosis, Kif11, a kinesin motor protein, promotes bipolar spindle formation and chromosome movement, and during interphase, Kif11 mediates diverse trafficking processes in the cytoplasm. In humans, inactivating mutations in KIF11 are associated with (1) retinal hypovascularization with or without microcephaly and (2) multi-organ syndromes characterized by variable combinations of lymphedema, chorioretinal dysplasia, microcephaly and/or mental retardation. To explore the pathogenic basis of KIF11-associated retinal vascular disease, we generated a Kif11 conditional knockout (CKO) mouse and investigated the consequences of early postnatal inactivation of Kif11 in vascular endothelial cells (ECs). The principal finding is that postnatal EC-specific loss of Kif11 leads to severely stunted growth of the retinal vasculature, mildly stunted growth of the cerebellar vasculature and little or no effect on the vasculature elsewhere in the central nervous system (CNS). Thus, in mice, Kif11 function in early postnatal CNS ECs is most significant in the two CNS regions—the retina and cerebellum—that exhibit the most rapid rate of postnatal growth, which may sensitize ECs to impaired mitotic spindle function. Several lines of evidence indicate that these phenotypes are not caused by reduced beta-catenin signaling in ECs, despite the close resemblance of the Kif11 CKO phenotype to that caused by EC-specific reductions in beta-catenin signaling. Based on prior work, defective beta-catenin signaling had been the only known mechanism responsible for monogenic human disorders of retinal hypovascularization. The present study implies that retinal hypovascularization can arise from a second and mechanistically distinct cause.