bluuebunny/crossref_metadata_2025_split huggingface dataset:
A cyanobacterial photorespiratory bypass model to enhance photosynthesis by rerouting photorespiratory pathway in C3 plants
Authors: Khurshid Ghazal , Abbassi Anum Zeb , Khalid Muhammad Farhan , Gondal Mahnoor Naseer , Naqvi Tatheer Alam , Shah Mohammad Maroof , Chaudhary Safee Ullah , Ahmad Raza 
Published: November 2020

AbstractPlants employ photosynthesis to produce sugars for supporting their growth. During photosynthesis, an enzyme Ribulose 1,5 bisphosphate carboxylase/oxygenase (Rubisco) combines its substrate Ribulose 1,5 bisphosphate (RuBP) with CO2 to produce phosphoglycerate (PGA). Alongside, Rubisco also takes up O2 and produce 2-phosphoglycolate (2-PG), a toxic compound broken down into PGA through photorespiration. Photorespiration is not only a resource-demanding process but also results in CO2 loss which affects photosynthetic efficiency in C3 plants. Here, we propose to circumvent photorespiration by adopting the cyanobacterial glycolate decarboxylation pathway into C3 plants. For that, we have integrated the cyanobacterial glycolate decarboxylation pathway into a kinetic model of C3 photosynthetic pathway to evaluate its impact on photosynthesis and photorespiration. Our results show that the cyanobacterial glycolate decarboxylation bypass model exhibits a 10% increase in net photosynthetic rate (A) in comparison with C3 model. Moreover, an increased supply of intercellular CO2 (Ci) from the bypass resulted in a 54.8% increase in PGA while reducing photorespiratory intermediates including glycolate (− 49%) and serine (− 32%). The bypass model, at default conditions, also elucidated a decline in phosphate-based metabolites including RuBP (− 61.3%). The C3 model at elevated level of inorganic phosphate (Pi), exhibited a significant change in RuBP (+ 355%) and PGA (− 98%) which is attributable to the low availability of Ci. Whereas, at elevated Pi, the bypass model exhibited an increase of 73.1% and 33.9% in PGA and RuBP, respectively. Therefore, we deduce a synergistic effect of elevation in CO2 and Pi pool on photosynthesis. We also evaluated the integrative action of CO2, Pi, and Rubisco carboxylation activity (Vcmax) on A and observed that their simultaneous increase raised A by 26%, in the bypass model. Taken together, the study potentiates engineering of cyanobacterial decarboxylation pathway in C3 plants to bypass photorespiration thereby increasing the overall efficiency of photosynthesis.
Elevated temperature, but not decreased pH, impairs reproduction in a temperate fish
Authors: Lopes Ana F. , Faria Ana M. , Dupont Sam 
Published: November 2020

AbstractFish reproductive success is linked to the ability of couples to mate and produce clutches that successfully hatch. Environmental stressors like high temperature and low pH can jeopardize this energetically costly process. In this study, exposure to high temperature and low pH was tested on a marine temperate species,Gobiusculus flavescens, to evaluate effects on reproductive performance. Breeding pairs were assigned to different temperatures (+ 0 °C, + 3 °C relative to in situ temperature) and pH levels (8.0, 7.6), in a cross-factorial design for a 3-month period. Reproduction activity, success, and paternal investment were measured throughout the exposure period. Results show reproduction is impaired by elevated temperature, while low pH had little impact. Breeding pairs under high temperature had 3% to 10% hatching success, up to 30% less eggs and eggs up to 20% smaller. Although paternal investment was not affected by tested parameters, males of breeding pairs exposed to elevated temperature had smaller gonadosomatic indexes, which might indicate a lack of investment in the reproductive process. Overall, results show that elevated temperature, expected more frequently in the near future, as a consequence of global warming, may impair key processes like reproduction in temperate fish, with potential consequences for fitness and population replenishment.
Inter-method reliability for determining total and soluble fluorides in child low-fluoride formula dentifrices
Authors: Bijle Mohammed Nadeem , Tsoi James , Ekambaram Manikandan , Lo Edward C. M. , Carey Clifton M. , Yiu Cynthia Kar Yung 
Published: November 2020

AbstractThe study aimed to compare three methods for determining total (TF) and total soluble fluorides (TSF) in 5 child formula dentifrices (CFD) using Inter-method reliability (IMR) statistical approach. The methods were direct acid-hydrolysis (DM), the least-time-consuming method; Modified direct acid-hydrolysis with standard-addition method (MDM), ISO 19448:2018 method; and modified Taves acid-HMDS diffusion analysis (TAD), the claimed gold standard method. A significant difference in the mean difference was observed for all methods at all levels (p < 0.001), except DM and TAD for TF (p = 0.622). A proportional bias was discerned in the agreement distribution between DM and TAD for TF (p < 0.001). The ICC analysis identified significant reliability between all measurements, irrespective of the model, measure, and fluoride type (p < 0.001). For TF and TSF, the IMR between DM and TAD was lower than MDM and TAD for consistency/absolute agreement reliability at single/average measures. The reliability measure for DM and MDM was higher than MDM and TAD for TSF, but was lower than MDM and TAD for TF. The ICC measure for DM-TAD was significantly lower than DM-MDM and MDM-TAD (p < 0.05). The ISO 19448:2018 MDM is a reliable test that can be used as an alternative to TAD/DM for determining TF/TSF in CFD.
Predictors and prognoses of Willisian collateral failure during mechanical thrombectomy
Authors: Lee Seong-Joon , Hwang Yang-Ha , Hong Ji Man , Choi Jin Wook , Kang Dong-Hun , Kim Yong-Won , Kim Yong-Sun , Hong Jeong-Ho , Yoo Joonsang , Kim Chang-Hyun , Ovbiagele Bruce , Demchuk Andrew , Sohn Sung-Il , Lee Jin Soo 
Published: November 2020

AbstractDuring mechanical thrombectomy in the anterior cerebral circulation, thrombus embolization resulting in Willisian collateral failure may lead to critical stroke outcomes due to a shutdown of leptomeningeal collaterals. We hypothesized that the outcomes of dynamic Willisian collateral failure (DWF), induced during mechanical thrombectomy, would be associated with grave outcomes. We evaluated this hypothesis in consecutive patients, between January 2011 and May 2016, who underwent mechanical thrombectomy for anterior circulation occlusions, with an onset-to-puncture of 24 h. Patients with initial Willisian collateral failure (IWF) were identified first, with remaining patients classified into the DWF and Willisian collateral sparing (WCS) groups. Comparative and multivariable analyses were performed to predict grave outcomes (3-month modified Rankin Scale score of 5–6). Among 567 patients, 37 were in the IWF group, 38 in the DWF group, and 492 in the WCS group. Compared to the WCS and DWF groups, the IWF group had a higher baseline National Institute of Health Stroke Scale score and lower Alberta Stroke Program Early CT Score. The prevalence of grave outcomes was similarly high in the IWF (48.6%) and DWF (47.4%) groups, but lower in the WCS group (22.0%; p < 0.001). IWF and DWF were independent risk factors for a grave outcome.
High dose vitamin D3 empowers effects of subcutaneous immunotherapy in a grass pollen-driven mouse model of asthma
Authors: Hesse Laura , van Ieperen N. , Petersen Arjen H. , Elberink J. N. G. Oude , van Oosterhout Antoon J. M. , Nawijn Martijn C. 
Published: November 2020

AbstractAllergen-specific immunotherapy (AIT) has the potential to provide long-term protection against allergic diseases. However, efficacy of AIT is suboptimal, while application of high doses allergen has safety concerns. The use of adjuvants, like 1,25(OH)2VitD3 (VitD3), can improve efficacy of AIT. We have previously shown that low dose VitD3 can enhance suppression of airway inflammation, but not airway hyperresponsiveness in a grass pollen (GP)-subcutaneous immunotherapy (SCIT) mouse model of allergic asthma. We here aim to determine the optimal dose and formulation of VitD3 for the GP SCIT. GP-sensitized BALBc/ByJ mice received three SCIT injections of VitD3-GP (30, 100, and 300 ng or placebo). Separately, synthetic lipids, SAINT, was added to the VitD3-GP-SCIT formulation (300 nmol) and control groups. Subsequently, mice were challenged with intranasal GP, and airway hyperresponsiveness, GP-specific IgE, -IgG1, and -IgG2a, ear-swelling responses (ESR), eosinophils in broncho-alveolar lavage fluid and lung were measured. VitD3 supplementation of GP-SCIT dose-dependently induced significantly enhanced suppression of spIgE, inflammation and hyperresponsiveness, while neutralizing capacity was improved and ESR were reduced. Addition of VitD3 further decreased Th2 cytokine responses and innate cytokines to allergens in lung tissue by GP-SCIT. However, addition of synthetic lipids to the allergen/VitD3 mixes had no additional effect on VitD3-GP-SCIT. We find a clear, dose dependent effect of VitD3 on GP-SCIT-mediated suppression of allergic inflammation and airway hyperresponsiveness. In contrast, addition of synthetic lipids to the allergen/VitD3 mix had no therapeutic effect. These studies underscore the relevance of VitD3 as an adjuvant to improve clinical efficacy of SCIT treatment regimens.
Comparison of hourly aerosol retrievals from JAXA Himawari/AHI in version 3.0 and a simple customized method
Authors: Xu Weiwei , Wang Wei , Chen Biyan 
Published: November 2020

AbstractAdvanced Himawari imager (AHI) carried on the new-generation geostationary meteorological Himawari-8 satellite of Japan has been generating aerosol observations with a high temporal resolution since 7 July 2015. However, the previous studies lack a comprehensive quality assessment and spatial coverage analysis of AHI hourly aerosol products (level 3 version 3.0) across the full disk scan. The monitoring accuracy of different AHI aerosol products (AODpure and AODmerged) and a simple customized product (AODmean) was evaluated against Aerosol Robotic Network (AERONET) and Maritime Aerosol Network (MAN) observations from May 2016 to February 2019 in this study. Results showed that AHI AODmean demonstrates a better agreement to AERONET AOD measurements than AODpure and AODmerged over land (R = 0.81, bias =  − 0.011) and all the AHI land retrievals present a significant regional performance differences, while the relatively better performance is observed in AODmerged over the coastal regions (R = 0.89, bias = 0.053). Over ocean, AHI exhibited overall overestimation in retrieving AOD against MAN observations and the relatively lower uncertainties were found in AODpure retrievals (R = 0.96, bias = 0.057). The hourly comparisons in different AHI products demonstrated a robust performance in the late afternoon (16:00–17:00 LT) over land and around the noon (10:00–13:00 LT) over coast. AHI AOD products indicated an obvious underestimation when compared to MODIS AOD retrievals over both land and ocean. Furthermore, the performance differences of AHI AOD products have also affected by the vegetation cover, pollution levels and relative humidity. For spatiotemporal coverage, the results of different AHI products demonstrated that AODmean can achieve relatively higher coverage than AODpure and AODmerged, and AHI retrievals present significant regional differences in coverage capability.
SOFA score and short-term mortality in acute decompensated heart failure
Authors: Elias Adi , Agbarieh Reham , Saliba Walid , Khoury Johad , Bahouth Fadel , Nashashibi Jeries , Azzam Zaher S. 
Published: November 2020

AbstractAcute decompensated heart failure (ADHF) is one of the leading causes for hospitalization and mortality. Identifying high risk patients is essential to ensure proper management. Sequential Organ Function Assessment Score (SOFA) is considered an excellent score to predict short-term mortality in sepsis and other life-threatening conditions. To assess the capability of SOFA score in predicting short-term mortality in ADHF. We retrospectively identified patients with first hospitalization with primary diagnosis of ADHF between the years (2008–2018). The SOFA score was calculated for all patients. A total 3232 patients were included in the study. The SOFA score was significantly associated with in-hospital mortality and 30-day mortality. The odds ratios for 1-point increase in the SOFA score were 1.86 (95% CI 1.68–1.96) and 1.627 (95% CI 1.523–1.737) respectively. The SOFA Score demonstrated a good predictive accuracy. The areas under the curve of receiver operating characteristic curves for in-hospital mortality and 30-day mortality were 0.765 (95% CI 0.733–0.798) and 0.706 (95% CI 0.676–0.736) respectively. SOFA score is associated with increased risk of short-term mortality in ADHF. SOFA can be used as a complementary risk score to screen high risk patients who need strict monitoring.
Fluctuation in radioresponse of HeLa cells during the cell cycle evaluated based on micronucleus frequency
Authors: Shimono Hiroaki , Kaida Atsushi , Homma Hisao , Nojima Hitomi , Onozato Yusuke , Harada Hiroyuki , Miura Masahiko 
Published: November 2020

AbstractIn this study, we examined the fluctuation in radioresponse of HeLa cells during the cell cycle. For this purpose, we used HeLa cells expressing two types of fluorescent ubiquitination-based cell cycle indicators (Fucci), HeLa-Fucci (CA)2 and HeLa-Fucci (SA), and combined this approach with the micronucleus (MN) assay to assess radioresponse. The Fucci system distinguishes cell cycle phases based on the colour of fluorescence and cell morphology under live conditions. Time-lapse imaging allowed us to further identify sub-positions within the G1 and S phases at the time of irradiation by two independent means, and to quantitate the number of MNs by following each cell through M phase until the next G1 phase. Notably, we found that radioresponse was low in late G1 phase, but rapidly increased in early S phase. It then decreased until late S phase and increased in G2 phase. For the first time, we demonstrated the unique fluctuation of radioresponse by the MN assay during the cell cycle in HeLa cells. We discuss the difference between previous clonogenic experiments using M phase-synchronised cell populations and ours, as well as the clinical implications of the present findings.
Uniform-thickness electrospun nanofiber mat production system based on real-time thickness measurement
Authors: Ryu Hyun Il , Koo Min Seok , Kim Seokjun , Kim Songkil , Park Young-Ah , Park Sang Min 
Published: November 2020

AbstractElectrospinning is a simple versatile process used to produce nanofibers and collect them as a nanofiber mat. However, due to bending instability, electrospinning often produces a nanofiber mat with non-uniform mat thickness. In this study, we developed a uniform-thickness electrospun nanofiber mat (UTEN) production system with a movable collector based on real-time thickness measurement and thickness feedback control. This system is compatible with a collector with void regions such as a mesh-type collector, two-parallel-metal-plate collector, and ring-type collector, which facilitates the measurement of light transmittance across the produced nanofiber mat during electrospinning. A real-time measurement system was developed to measure light transmittance and convert it to the thickness of the nanofiber mat in real time using the Beer–Lambert law. Thickness feedback control was achieved by repeating the following sequences: (1) finding an optimal position of the movable collector based on the measured thickness of the nanofiber mat, (2) shifting the collector to an optimal position, and (3) performing electrospinning for a given time step. We found that the suggested thickness feedback control algorithm could significantly decrease the non-uniformity of the nanofiber mat by reducing the standard deviation by more than 8 and 3 times for the numerical simulation and experiments, respectively, when compared with the conventional electrospinning. As a pioneering research, this study will contribute to the development of an electrospinning system to produce robust and reliable nanofiber mats in many research and industrial fields such as biomedicine, environment, and energy.
Printing special surface components for THz 2D and 3D imaging
Authors: Yan Bo , Wang Zhigang , Zhao Xing , Lin Lie , Wang Xiaolei , Gong Cheng , Liu Weiwei 
Published: November 2020

AbstractThe paper reports an off-axis large focal depth THz imaging system which consists of three 3D printed special surface components (two aspherical mirrors and an axicon). Firstly, the optical design software is used to design and optimize the aspherical parabolic mirror. Secondly, the optimized mirror is prepared by a 3D printing and metal cladding method. Thirdly, a THz axicon is designed for generation of quasi-Bessel Beam and a new geometric theoretical model of oblique incident light for axicon is established. Finally, the imaging system based on the special surface components is constructed. Its maximum diffraction-free distance is about 60 mm, which is 6 times higher than the traditional system. To verify the effectiveness, THz two-dimensional imaging experiments and three-dimensional computed tomography experiment are carried out. The results are consistent with the design and calculations.
Comparisons of conventional and novel anthropometric obesity indices to predict metabolic syndrome among vegetarians in Malaysia
Authors: Ching Yuan Kei , Chin Yit Siew , Appukutty Mahenderan , Gan Wan Ying , Chan Yoke Mun 
Published: November 2020

AbstractOur study aimed to compare the ability of anthropometric obesity indices to predict MetS and to determine the sex-specific optimal cut-off values for MetS among Malaysian vegetarians. Body weight, height, waist circumference (WC), blood pressure (BP), fasting venous blood sample were collected from 273 vegetarians in Selangor and Kuala Lumpur, Malaysia. The abilities of body mass index (BMI), body fat percentage (BF%), waist to height ratio (WHtR), lipid accumulation product (LAP), visceral adiposity index (VAI), a body shape index (ABSI), and body roundness index (BRI) to identify MetS were tested using receiver operating characteristic (ROC) curve analyses. MetS was defined according to the Joint Interim Statement 2009. The ROC curve analyses show that BMI, BF%, WHtR, LAP and VAI were able to discriminate MetS in both sexes. LAP was a better predictor to predict MetS, followed by WHtR for male and female vegetarians. The suggested WHtR’s optimal cut-offs and LAP’s optimal cut-offs for MetS for male and female vegetarians were 0.541, 0.532, 41.435 and 21.743, respectively. In conclusion, LAP was a better predictor to predict MetS than other anthropometric obesity indices. However, WHtR could be an alternative obesity index in large epidemiology survey due to its convenient and cost-effective characteristics.
Characteristics of secondary epiretinal membrane due to peripheral break
Authors: Lee Geun Woo , Lee Sang Eun , Han Sun Hyup , Kim Sang Jin , Kang Se Woong 
Published: November 2020

AbstractThis study aimed to investigate morphological differences between idiopathic epiretinal membrane (ERM) and secondary ERM due to peripheral break (SEPB) and to identify clinical characteristics in eyes with SEPB to facilitate peripheral retinal examination. The retrospective cross-sectional study involved 93 consecutive eyes in 91 patients who underwent ERM removal surgery. Eyes were divided into two groups: the macular pucker group and the idiopathic ERM group. En-face Optical Coherence Tomography (OCT) images, fundus photographs, severity of metamorphopsia (M-score) and clinical characteristics of each group were compared. ERM extent and eccentricity (ratio of the shortest and longest distances from the foveal center to the boundary) were obtained through en-face OCT imaging. Fundus photographs were used to judge whether the membrane was turbid or not. Patients with SEPB were younger than patients with idiopathic ERM (61.3 ± 7.5 vs. 66.6 ± 8.3 years; p < 0.05). Preoperative M-score and myopic refractive error, axial length were also significantly higher in the macular pucker group than in the idiopathic ERM group (all p < 0.05). There was no difference in ERM extent between the two groups. The incidence of ERM eccentricity was 23 of the 34 eyes (67.6%) in the SEPB group and 26 of the 59 eyes (44.1%) in the idiopathic ERM group (p < 0.05). The incidence of turbid ERM was 18 of the 34 eyes (52.9%) in the SEPB group and 10 of the 59 eyes (16.9%) in the idiopathic ERM group (p < 0.01). The SEPB group, compared with the idiopathic ERM group, tended to have eccentric, turbid ERM at a younger age and with more severe metamorphopsia and myopic refractive error.
Low serum calcium: a new, important indicator of COVID-19 patients from mild/moderate to severe/critical
Authors: Zhou Xi , Chen Dong , Wang Lan , Zhao Yuanyuan , Wei Lai , Chen Zhishui , Yang Bo 
Published: November 2020

Abstract
Background: Coronavirus disease 2019 (COVID-19) virus is still spreading, finding out the initial hits of viral infection is important to minimize the mild/moderate population, prevent disease aggravation and organs dysfunction.
Objective: We investigated COVID-19 patients with different serum calcium levels.
Design: We checked the serum calcium level of the patients based on days after symptom onset as well as the severity of COVID-19. We also checked multiorgan injuries and immune cytokines level in their blood.
Results: Both mild/moderate and severe critical cases we observed showed low calcium level in the early stage of viral infection, while the severe/critical cases showed significant lower calcium level than mild/moderate cases in the early stage. We also found that low calcium level related to severe/critical multiorgan injuries especially in the mild/moderate population. Proinflammatory cytokine IL-6 also correlated to calcium change in both mild/moderate and severe/critical cases.
Conclusions: Our finding indicates that calcium balance is a primal hit of COVID-19 and a biomarker of clinical severity at the beginning of symptom onset. Calcium is closely associated with virus-associated multiple organ injuries and the increase in inflammatory cytokines. Our results provide a new, important indicator of COVID-19 patients from mild/moderate to severe/critical: serum calcium.
Discipline and methodological paradigm
Authors: Shchemeleva Irina 
Published: November 2020

AbstractIn contemporary academia, multilingual scholars using English as an additional language (EAL) are actively engaged in knowledge construction producing more research texts in English than native speakers (Hyland, 2016). Having a more general purpose to gain insights into the factors that influence multilingual scholars’ research writing practices in English, this case study seeks to explore how EAL users perceive disciplinary norms of epistemic stance expression in political science. It is based on interviews with 5 Russian political scientists and on the analysis of their research texts. The findings suggest that the participants do not seem to have a shared understanding of disciplinary norms regarding epistemic stance expression; however, their narratives highlight the importance of the methodological paradigm the texts belong to for their writing practices. The study is a contribution to the discussion of the role of the discipline in EAL scholars’ research writing practices and linguistic and rhetorical variability of research texts within one discipline. The results of this study have pedagogical implications for ERPP course designers and practitioners.