bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measurement of 10 fs pulses across the entire Visible to Near-Infrared Spectral Range
Authors: Johnson Allan S. , Amuah Emmanuel B. , Brahms Christian , Wall Simon 
Published: March 2020

AbstractTuneable ultrafast laser pulses are a powerful tool for measuring difficult-to-access degrees of freedom in materials science. In general these experiments require the ability to address resonances and excitations both above and below the bandgap of materials, and to probe their response at the timescale of the fastest non-trivial internal dynamics. This drives the need for ultrafast sources capable of delivering 10–15 fs duration pulses tuneable across the entire visible (VIS) and near infrared (NIR) range, 500– 3000 nm, as well as the characterization of these sources. Here we present a single frequency-resolved optical gating (FROG) system capable of self-referenced characterization of pulses with 10 fs duration across the entire VIS-NIR spectral range. Our system does not require auxiliary beams and only minor reconfiguration for different wavelengths. We demonstrate the system with measurements of pulses across the entire tuning range.
Intrauterine growth restriction in piglets alters blood cell counts and impairs cytokine responses in peripheral mononuclear cells 24 days post-partum
Authors: Amdi Charlotte , Lynegaard Julie C. , Thymann Thomas , Williams Andrew R. 
Published: March 2020

AbstractLarge litter sizes have resulted in more piglets being exposed to intrauterine growth restriction (IUGR). Growth restriction during fetal life is linked with lower growth efficiency and increased susceptibility to infections in postnatal life and IUGR may associate with an altered innate immune system. We investigated the haematological, thromboelastography and plasma biochemical profiles of IUGR and normal piglets as well as cytokine responses in peripheral blood mononuclear cells stimulated with lipopolysaccharide (LPS) at 24 days of age. Piglets were classified at birth based on their head morphology as either IUGR or normal. The present study showed a modulation of the immune function of IUGR pigs, characterized by an increase in neutrophil percentage and fibrinogen levels but a decrease in CD4+ T-cells. A lower level of LPS-induced IL-1β production was evident in IUGR pigs, suggesting immunological hypo-responsiveness. Furthermore, higher levels of reticulocytes, MCV and MCH and lower levels of erythrocytes in IUGR pigs suggest altered bone marrow hematopoiesis. All together, the results suggest a moderate suppression of the immune response of IUGR piglets, which may have implications for resistance to pathogen challenges in the post-weaning period. Serum metabolites and blood clotting profile did not differ between IUGR and normal piglets.
Gut microbiota manipulation during the prepubertal period shapes behavioral abnormalities in a mouse neurodevelopmental disorder model
Authors: Saunders Justin M. , Moreno José L. , Ibi Daisuke , Sikaroodi Masoumeh , Kang Dae Joong , Muñoz-Moreno Raquel , Dalmet Swati S. , García-Sastre Adolfo , Gillevet Patrick M. , Dozmorov Mikhail G. , Bajaj Jasmohan S. , González-Maeso Javier 
Published: March 2020

AbstractPrevious studies demonstrate an association between activation of the maternal immune system during pregnancy and increased risk of neurodevelopmental psychiatric conditions, such as schizophrenia and autism, in the offspring. Relatively recent findings also suggest that the gut microbiota plays an important role in shaping brain development and behavior. Here we show that maternal immune activation (MIA) accomplished by infection with a mouse-adapted influenza virus during pregnancy induced up-regulation of frontal cortex serotonin 5-HT2A receptor (5-HT2AR) density in the adult offspring, a phenotype previously observed in postmortem frontal cortex of schizophrenic subjects. 5-HT2AR agonist-induced head-twitch behavior was also augmented in this preclinical mouse model. Using the novel object recognition (NOR) test to evaluate cognitive performance, we demonstrate that MIA induced NOR deficits in adult offspring. Oral antibiotic treatment of prepubertal mice prevented this cognitive impairment, but not increased frontal cortex 5-HT2AR density or psychedelic-induced head-twitch behavior in adult MIA offspring. Additionally, gut microbiota transplantation from MIA mice produced behavioral deficits in antibiotic-treated mock mice. Adult MIA offspring displayed altered gut microbiota, and relative abundance of specific components of the gut microbiota, including Ruminococcaceae, correlated with frontal cortex 5-HT2AR density. Together, these findings provide a better understanding of basic mechanisms by which prenatal insults impact offspring brain function, and suggest gut-brain axis manipulation as a potential therapeutic approach for neurodevelopmental psychiatric conditions.
Validation of CTS5 model in large-scale breast cancer population and the impact of menopausal and HER2 status on its prognostic value
Authors: Wang Changjun , Chen Chang , Lin Yan , Zhou Yidong , Mao Feng , Zhu Hanjiang , Zhang Xiaohui , Shen Songjie , Huang Xin , Wang Xuefei , Zhao Bin , Yang Jing , Sun Qiang 
Published: March 2020

AbstractClinical Treatment Score post-5 years (CTS5) is a promising prognostic tool to evaluate late recurrence risk for breast cancer. Our study aimed to validate its prognostic value in large-scale population and explore the impact of menopausal and HER2 status on CTS5 model. We performed a retrospective cohort study using the Surveillance, Epidemiology, and End Results (SEER) database. Survival analyses were conducted to assess the prognostic value of CTS5 in different breast cancer subgroups in terms of overall survival (OS) and breast cancer specific survival (BCSS) after five years. A total of 23,168 breast cancer patients with positive hormone receptor (HoR) were enrolled. Postmenopausal and premenopausal patients were 13,686 and 9,482, respectively. Taking CTS5 score as a continuous variable, it had significant positive correlation with poor prognosis beyond five years in both postmenopausal and premenopausal subgroups. Nevertheless, for HER2+ postmenopausal patients, the model has less effective prognostic value on long-term BCSS [HR1.177 (95%CI 0.960–1.443), p = 0.117]. Using CTS5 score as a categorical variable, HER2- patients with high-risk level revealed significant poor survival in terms of both BCSS and OS, irrespective of menopausal status. Our study showed the CTS5 model could be a useful prognostic tool for predict long-term survival in HoR+/HER2- patients. And further large-scale studies are warranted to assess its prognostic value for HER2+ patients and develop novel prediction model for late recurrence risk estimation.
Cell cycle synchronisation using thiazolidinediones affects cellular glucose metabolism and enhances the therapeutic effect of 2-deoxyglucose in colon cancer
Authors: Yoon Joon-Kee , Byeon Hye Eun , Ko Seung Ah , Park Bok-Nam , An Young-Sil , Lee Ho-Young , Lee Youn Woo , Lee Su Jin 
Published: March 2020

AbstractThe effect of cell cycle synchronisation on glucose metabolism in cancer cells is not known. We assessed how cell cycle synchronisation by thiazolidinediones (TZDs) can affect glucose uptake by cancer cells and investigated the anti-cancer effect of combination therapy with TZDs and 2-deoxy-glucose (2-DG) in colon cancer cells and in mouse xenograft models. Troglitazone (58.1 ± 2.0 vs 48.6 ± 1.3%, p = 0.002) or pioglitazone (82.9 ± 1.9 vs 61.6 ± 3.4%, p < 0.001) induced cell cycle arrest in SW480 cells at G1 phase. Western blot analysis showed the degradation of cyclin D1 and CDK4, and an increase in the expression levels of p21 and p27 after TZDs treatment. Withdrawal of troglitazone treatment induced significant increase in cellular 3H-DG uptake (141.5% ± 12.9% of controls) and membrane GLUT1 expression levels (146.3% of controls) by 24 h; 1 mM 2-DG treatment alone decreased cell survival by 5.8% as compared with the controls.; however, combination therapy enhanced the anti-tumour effects to 34.6% or 20.3% as compared with control cells. In vivo, each combination treatment group showed significant anti-tumour effects unlike the 2-DG alone group. Cell cycle synchronisation using TZDs induced cellular glucose uptake, which significantly enhanced the therapeutic effect of 2-DG in colon cancer.
A novel MRI analysis for assessment of microvascular vasomodulation in low-perfusion skeletal muscle
Authors: Zakher Eric , Ganesh Tameshwar , Cheng Hai-Ling Margaret 
Published: March 2020

AbstractCompromised microvascular reactivity underlies many conditions and injuries, but its assessment remains difficult, particularly in low perfusion tissues. In this paper, we develop a new mathematical model for the assessment of vasomodulation in low perfusion settings. A first-order model was developed to approximate changes in T1 relaxation times as a result of vasomodulation. Healthy adult rats (N = 6) were imaged on a 3-Tesla clinical MRI scanner, and vasoactive response was probed on gadofosveset using hypercapnic gases at 20% and 5% CO2 to induce vasoconstriction and vasodilation, respectively. MRI included dynamic 3D T1 mapping and T1-weighted images during gas challenge; heart rate was continuously monitored. Laser Doppler perfusion measurements were performed to corroborate MRI findings. The model was able to identify hypercapnia-mediated vasoconstriction and vasodilation through the partial derivative $$\frac{\partial {T}_{1}}{\partial t}$$∂T1∂t. MRI on animals revealed gradual vasoconstriction in the skeletal muscle bed in response to 20% CO2 followed by gradual vasodilation on transitioning to 5% CO2. These trends were confirmed on laser Doppler perfusion measurements. Our new mathematical model has the potential for detecting microvascular dysfunction that manifests in the early stages across multiple metabolic and ischemic pathologies.
Zeolite Cotton in Tube: A Simple Robust Household Water Treatment Filter for Heavy Metal Removal
Authors: Chen Xutao , Yu Lisha , Zou Shihui , Xiao Liping , Fan Jie 
Published: March 2020

AbstractIt is challenging to develop a low-cost household water treatment (HWT) that simultaneously deliver an effective and robust way for safe and reliable water supply. Here, we report a simple flow-through filter made by zeolite-cotton packing in a tube (ZCT) as low-cost HWT device to remove heavy metal ions from contaminated water. The zeolite-cotton is fabricated by an on-site template-free growth route that tightly binds mesoporous single-crystal chabazite zeolite onto the surface of cotton fibers. As a result, the ZCT set-up with optimized diameter achieves both high adsorption efficiency, proper flow rate, reliable supply and strong stability at the same time. After flowed through the set up packed with 10 g of zeolite-cotton, 65 mL 1000 ppm Cu2+ solution was purified down to its safety limit (<1 ppm). Notably, their efficiency remains unaltered when filtering several ions simultaneously. In a simulated purification process, 8 L of water contaminated by Cu2+, Cd2+ and Pb2+ could be transformed into drinking water and it enables the removal of heavy metals to concentrations of below 5 ppb (μg L−1). We also show that the ZCT can be used for disinfection by introducing Ag-exchanged zeolite-cotton without contaminating the water with Ag ions (<0.05 ppm).
Modernizing and designing evaluation frameworks for connected sensor technologies in medicine
Authors: Coravos Andrea , Doerr Megan , Goldsack Jennifer , Manta Christine , Shervey Mark , Woods Beau , Wood William A. 
Published: March 2020

AbstractThis manuscript is focused on the use of connected sensor technologies, including wearables and other biosensors, for a wide range of health services, such as collecting digital endpoints in clinical trials and remotely monitoring patients in clinical care. The adoption of these technologies poses five risks that currently exceed our abilities to evaluate and secure these products: (1) validation, (2) security practices, (3) data rights and governance, (4) utility and usability; and (5) economic feasibility. In this manuscript we conduct a landscape analysis of emerging evaluation frameworks developed to better manage these risks, broadly in digital health. We then propose a framework specifically for connected sensor technologies. We provide a pragmatic guide for how to put this evaluation framework into practice, taking lessons from concepts in drug and nutrition labels to craft a connected sensor technology label.
Single-cell RNA sequencing reveals a heterogeneous response to Glucocorticoids in breast cancer cells
Authors: Hoffman Jackson A. , Papas Brian N. , Trotter Kevin W. , Archer Trevor K. 
Published: March 2020

AbstractSteroid hormone receptors such as the Glucocorticoid Receptor (GR) mediate transcriptional responses to hormones and are frequently targeted in the treatment of human diseases. Experiments using bulk populations of cells have provided a detailed picture of the global transcriptional hormone response but are unable to interrogate cell-to-cell transcriptional heterogeneity. To examine the glucocorticoid response in individual cells, we performed single cell RNA sequencing (scRNAseq) in a human breast cancer cell line. The transcriptional response to hormone was robustly detected in individual cells and scRNAseq provided additional statistical power to identify over 100 GR-regulated genes that were not detected in bulk RNAseq. scRNAseq revealed striking cell-to-cell variability in the hormone response. On average, individual hormone-treated cells showed a response at only 30% of the total set of GR target genes. Understanding the basis of this heterogeneity will be critical for the development of more precise models of steroid hormone signaling.
Podosome formation promotes plasma membrane invagination and integrin-β3 endocytosis on a viscous RGD-membrane
Authors: Cao Fakun , Zhou Yuhuan , Liu Xiaoting , Yu Cheng-han 
Published: March 2020

AbstractIntegrin receptors orchestrate cell adhesion and cytoskeletal reorganization. The endocytic mechanism of integrin-β3 receptor at the podosome remains unclear. Using viscous RGD-membrane as the model system, here we show that the formation of podosome-like adhesion promotes Dab2/clathrin-mediated endocytosis of integrin-β3. Integrin-β3 and RGD ligand are endocytosed from the podosome and sorted into the endosomal compartment. Inhibitions of podosome formation and knockdowns of Dab2 and clathrin reduce RGD endocytosis. F-actin assembly at the podosome core exhibits protrusive contact towards the substrate and results in plasma membrane invaginations at the podosome ring. BIN1 specifically associates with the region of invaginated membrane and recruits DNM2. During the podosome formation, BIN1 and DNM2 synchronously enrich at the podosome ring and trigger clathrin dissociation and RGD endocytosis. Knockdowns of BIN1 and DNM2 suppress RGD endocytosis. Thus, plasma membrane invagination caused by F-actin polymerization promotes BIN1-dependent DNM2 recruitment and facilitate integrin-β3 endocytosis at the podosome.
Absence of Y-pocket in 1-Fe Brillouin zone and reversed orbital occupation imbalance in FeSe
Authors: Huh S. S. , Seo J. J. , Kim B. S. , Cho S. H. , Jung J. K. , Kim S. , Kwon C. I. , Kim Jun Sung , Koh Y. Y. , Kyung W. S. , Denlinger J. D. , Kim Y. H. , Chae B. N. , Kim N. D. , Kim Y. K. , Kim C. 
Published: March 2020

AbstractThe FeSe nematic phase has been the focus of recent research on iron-based superconductors (IBSs) due to its unusual properties, which are distinct from those of the pnictides. A series of theoretical/experimental studies were performed to determine the origin of the nematic phase. However, they yielded conflicting results and caused additional controversies. Here, we report the results of angle-resolved photoemission and X-ray absorption spectroscopy studies on FeSe detwinned by a piezo stack. We fully resolved band dispersions with orbital characters near the Brillouin zone (BZ) corner, and revealed an absence of any Fermi pocket at the Y point in the 1-Fe BZ. In addition, the occupation imbalance between $${d}_{{xz}}$$

d

x
z

and $${d}_{{yz}}$$

d

y
z

orbitals was the opposite of that of iron pnictides, consistent with the identified band characters. These results resolve issues associated with the FeSe nematic phase and shed light on the origin of the nematic phase in IBSs.
Inflammation: major denominator of obesity, Type 2 diabetes and Alzheimer’s disease-like pathology?
Authors: Kacířová Miroslava , Zmeškalová Anna , Kořínková Lucia , Železná Blanka , Kuneš Jaroslav , Maletínská Lenka 
Published: March 2020

AbstractAdipose tissue is an active metabolic organ that contributes to processes such as energy storage and utilization and to the production of a number of metabolic agents, such as adipokines, which play a role in inflammation. In this review, we try to elucidate the connections between peripheral inflammation at obesity and Type 2 diabetes and the central inflammatory process. Multiple lines of evidence highlight the importance of peripheral inflammation and its link to neuroinflammation, which can lead to neurodegenerative diseases such as dementia, Alzheimer’s disease (AD) and Parkinson’s disease. In addition to the accumulation of misfolded amyloid beta (Aβ) peptide and the formation of the neurofibrillary tangles of hyperphosphorylated tau protein in the brain, activated microglia and reactive astrocytes are the main indicators of AD progression. They were found close to Aβ plaques in the brains of both AD patients and rodent models of Alzheimer’s disease-like pathology. Cytokines are key players in pro- and anti-inflammatory processes and are also produced by microglia and astrocytes.The interplay of seemingly unrelated pathways between the periphery and the brain could, in fact, have a common denominator, with inflammation in general being a key factor affecting neuronal processes in the brain. An increased amount of white adipose tissue throughout the body seems to be an important player in pro-inflammatory processes. Nevertheless, other important factors should be studied to elucidate the pathological processes of and the relationship among obesity, Type 2 diabetes and neurodegenerative diseases.
A degree-100 lunar gravity model from the Chang’e 5T1 mission
Authors: Yan Jianguo , Liu Shanhong , Xiao Chi , Ye Mao , Cao Jianfeng , Harada Yuji , Li Fei , Li Xie , Barriot Jean-Pierre 
Published: March 2020

Context.Chinese lunar missions have grown in number over the last ten years, with an increasing focus on radio science investigations. In previous work, we estimated two lunar gravity field models, CEGM01 and CEGM02. The recently lunar mission, Chang’e 5T1, which had an orbital inclination between 18 and 68 degrees, and collected orbital tracking data continually for two years, made an improved gravity field model possible.Aims.Our aim was to estimate a new lunar gravity field model up to degree and order 100, CEGM03, and a new tidal Love number based on the Chang’e 5T1 tracking data combined with the historical tracking data used in the solution of CEGM02. The new model makes use of tracking data with this particular inclination, which has not been used in previous gravity field modeling.Methods.The solution for this new model was based on our in-house software, LUGREAS. The gravity spectrum power, post-fit residuals after precision orbit determination (POD), lunar surface gravity anomalies, correlations between parameters, admittance and coherence with topography model, and accuracy of POD were analyzed to validate the new CEGM03 model.Results.We analyzed the tracking data of the Chang’e 5T1 mission and estimated the CEGM03 lunar gravity field model. We found that the two-way Doppler measurement accuracy reached 0.2 mm s−1with 10 s integration time. The error spectrum shows that the formal error for CEGM03 was at least reduced by about 2 times below the harmonic degree of 20, when compared to the CEGM02 model. The admittance and correlation of gravity and topography was also improved when compared to the correlations for the CEGM02 model. The lunar potential Love numberk2was estimated to be 0.02430±0.0001 (ten times the formal error).Conclusions.From the model analysis and comparison of the various models, we identified improvements in the CEGM03 model after introducing Chang’e 5T1 tracking data. Moreover, this study illustrates how the low and middle inclination orbits could contribute better accuracy for a low degree of lunar gravity field.
Skill- und Grade-Mix in der Pflege (Teil 1)
Authors: Gumpenberger Franz 
Published: March 2020

ZUSAMMENFASSUNGDas gesamte Gesundheitswesen in Österreich befindet sich derzeit in einem markanten Wandel. Soziodemografische Entwicklungen mit einer Zunahme der Zahl von chronischen Erkrankungen, neue medizinische Errungenschaften sowie verkürzte Krankenhausaufenthalte und die damit verbundene steigende Leistungsdichte verlangen von allen Berufsgruppen im Gesundheitswesen fundierte Fachkenntnisse und außerordentliche Fähigkeiten. Dazu werden Diskussionen über die Finanzierbarkeit und Personalengpässe aller Professionen geführt. Geänderte Rahmenbedingungen im Gesundheits- und Krankenpflegegesetz in Österreich eröffnen dahingehend aber neue Zuständigkeits- und Kompetenzbereiche für Pflegende. So wurden neben dem gehobenen Dienst für Gesundheits- und Krankenpflege auch die Berufsgruppen der Pflegeassistenz und der Pflegefachassistenz etabliert. Um eine langfristige und hochwertige pflegerische Versorgung in Gesundheitseinrichtungen gewährleisten zu können, müssen künftig Neuanpassungen der Aufgabenbereiche oder auch Tätigkeitsverschiebungen innerhalb dieser Berufsgruppen stattfinden. Überlegungen, wer welche Tätigkeiten verrichtet, müssen künftig in einem konzeptgeleiteten Skill- und Grade-Mix angestellt werden. Auch im Versorgungsbereich der Dialysestation eines Krankenhauses sind solche Überlegungen unumgänglich und mögliche Zukunftsmodelle richtungsweisend.
Microvascular Effects of Echinodorus grandiflorus on Cardiovascular Disorders
Authors: Gomes Fabiana , Marques André M. , Nathalie Obadia , Lessa Marcos Adriano , Tibiriçá Eduardo , Figueiredo Maria Raquel , Estato Vanessa 
Published: March 2020

Abstract
Echinodorus grandiflorus is a semiaquatic plant native to Brazil and belongs to the Alismataceae family. Infusion preparations of the leaves of this plant are often used due to its diuretic, blood pressure lowering, and anti-inflammatory properties. Our aim was to investigate the effects of chronic treatment with the crude hydroalcoholic extract of E. grandiflorus on central and peripheral microvascular changes induced in a model of hypertension and diabetes. The hemodynamic and microvascular effects of E. grandiflorus extract (50, 100, or 200 mg/kg/day for 28 days) or the isolated major diterpene from E. grandiflorus (3 to 10 mg/kg i. v.) were evaluated in spontaneously hypertensive rats using tail plethysmography and intravital fluorescence videomicroscopy, respectively, and were compared to vehicle-treated normotensive Wistar-Kyoto rats. We also investigated the protective effects of chronic treatment with E. grandiflorus (100 mg/kg/day) in brain capillary density and leukocyte-endothelium interactions on the brain vessels of DM-spontaneously (DM: diabetes mellitus) hypertensive rats. Chronically treating spontaneously hypertensive rats with increasing doses of crude hydroalcoholic E. grandiflorus extract resulted in significant dose-dependent reductions in systolic blood pressure and an anti-inflammatory effect on the brain microcirculation of DM-spontaneously hypertensive rat animals. Using laser speckle contrast imaging, we observed that intravenous administration of the major isolated clerodane diterpene metabolite (1 – 10 mg/kg) increased microvascular blood flow by 25% in spontaneously hypertensive rat skeletal muscle. The results of this study show that E. grandiflorus extracts can be useful in the prevention and reduction of microcirculatory damage in arterial hypertension and other diseases that involve microvascular dysfunction.