bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell-mediated immune response and protective efficacy of porcine reproductive and respiratory syndrome virus modified-live vaccines against co-challenge with PRRSV-1 and PRRSV-2
Authors: Madapong Adthakorn , Saeng-chuto Kepalee , Boonsoongnern Alongkot , Tantituvanont Angkana , Nilubol Dachrit 
Published: February 2020

AbstractCell-mediated immunity (CMI), IL-10, and the protective efficacy of modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines (MLV) against co-challenge with PRRSV-1 and PRRSV-2 (HP-PRRSV) were investigated. Seventy, PRRSV-free, 3-week old, pigs were allocated into 7 groups. Six groups were intramuscularly vaccinated with MLV, including Porcilis (PRRSV-1 MLV, MSD Animal Health, The Netherlands), Amervac (PRRSV-1 MLV, Laboratorios Hipra, Spain), Fostera (PRRSV-2 MLV, Zoetis, USA), Ingelvac PRRS MLV and Ingelvac PRRS ATP (PRRSV-2, Boehringer Ingelheim, USA), and Prime Pac PRRS (PRRSV-2 MLV, MSD Animal Health, The Netherlands). Unvaccinated pigs were left as control. Lymphocyte proliferative response, IL-10 and IFN-γ production were determined. At 35 days post-vaccination (DPV), all pigs were inoculated intranasally with 2 ml of each PRRSV-1 (105.4 TCID50/ml) and PRRSV-2 (105.2 TCID50/ml, HP-PRRSV). Following challenge, sera were quantitatively assayed for PRRSV RNA. Pigs were necropsied at 7 days post-challenge. Viremia, macro- and microscopic lung lesion together with PRRSV antigen presence were evaluated in lung tissues. The results demonstrated that, regardless of vaccine genotype, CMI induced by all MLVs was relatively slow. Increased production of IL-10 in all vaccinated groups was observed at 7 and 14 DPV. Pigs in Amervac, Ingelvac MLV and Ingelvac ATP groups had significantly higher levels of IL-10 compared to Porcilis, Fostera and Prime Pac groups at 7 and 14 DPV. Following challenge, regardless to vaccine genotype, vaccinated pigs had significantly lower lung lesion scores and PRRSV antigens than those in the control group. Both PRRSV-1 and PRRSV-2 RNA were significantly reduced. Prime Pac pigs had lowest PRRSV-1 and PRRSV-2 RNA in serum, and micro- and macroscopic lung lesion scores (p < 0.05) compared to other vaccinated groups. In conclusion, PRRSV MLVs, regardless of vaccine genotype, can reduce viremia and lung lesions following co-challenge with PRRSV-1 and PRRSV-2 (HP-PRRSV). The main difference between PRRSV MLV is the production of IL-10 following vaccination.
Drivers of benthic metacommunity structure along tropical estuaries
Authors: Alves Andreia Teixeira , Petsch Danielle Katharine , Barros Francisco 
Published: February 2020

AbstractCommunity structure of many systems changes across space in many different ways (e.g., gradual, random or clumpiness). Accessing patterns of species spatial variation in ecosystems characterized by strong environmental gradients, such as estuaries, is essential to provide information on how species respond to them and for identification of potential underlying mechanisms. We investigated how environmental filters (i.e., strong environmental gradients that can include or exclude species in local communities), spatial predictors (i.e., geographical distance between communities) and temporal variations (e.g., different sampling periods) influence benthic macroinfaunal metacommunity structure along salinity gradients in tropical estuaries. We expected environmental filters to explain the highest proportion of total variation due to strong salinity and sediment gradients, and the main structure indicating species displaying individualistic response that yield a continuum of gradually changing composition (i.e., Gleasonian structure). First we identified benthic community structures in three estuaries at Todos os Santos Bay in Bahia, Brazil. Then we used variation partitioning to quantify the influences of environmental, spatial and temporal predictors on the structures identified. More frequently, the benthic metacommunity fitted a quasi-nested pattern with total variation explained by the shared influence of environmental and spatial predictors, probably because of ecological gradients (i.e., salinity decreases from sea to river). Estuarine benthic assemblages were quasi-nested likely for two reasons: first, nested subsets are common in communities subjected to disturbances such as one of our estuarine systems; second, because most of the estuarine species were of marine origin, and consequently sites closer to the sea would be richer while those more distant from the sea would be poorer subsets.
The association between an increase in glucose levels and armed conflict-related stress: A population-based study
Authors: Yitshak-Sade Maayan , Mendelson Nitsan , Novack Victor , Codish Shlomi , Liberty Idit F. 
Published: February 2020

AbstractStudies have shown stress may lead to diabetes-related morbidities. In recent years during enhanced hostility periods, the population of Southern Israel experienced alert sirens and rocket fire on a daily basis. We investigated whether the exposure to these stressful circumstances, which peaked during three large military operations (MO), was associated with increased glucose levels among the civilian population. We included all fasting serum glucose tests taken between 2007–2014, of Clalit Health Services members in Southern Israel who had at least one fasting glucose test during an MO period and at least one test drawn at other times. We analyzed the association between MO periods and glucose using linear mixed-effects models. We included 408,706 glucose tests (10% during MO periods). Among subjects who reside in proximity to Gaza, glucose levels were 2.10% (95% CI 1.24%; 2.97%) higher in MO days compared to other times. A weaker effect was observed among subjects in more remote locations. In conclusion, we found stress to be associated with increased fasting glucose levels, especially among those who reside in locations in which the intensity of the threat is higher. Since glucose may be a marker of the population at cardiovascular risk, further studies are required.
Significant improvement in catalytic activity and enantioselectivity of a Phaseolus vulgaris epoxide hydrolase, PvEH3, towards ortho-cresyl glycidyl ether based on the semi-rational design
Authors: Zhang Chen , Liu Youyi , Li Chuang , Xu Yaohui , Su Yongjun , Li Jinping , Zhao Jun , Wu Minchen 
Published: February 2020

AbstractThe investigation of substrate spectrum towards five racemic (rac-) aryl glycidyl ethers (1a–5a) indicated that E. coli/pveh3, an E. coli BL21(DE3) transformant harboring a PvEH3-encoding gene pveh3, showed the highest EH activity and enantiomeric ratio (E) towards rac-3a. For efficiently catalyzing the kinetic resolution of rac-3a, the activity and E value of PvEH3 were further improved by site-directed mutagenesis of selected residues. Based on the semi-rational design of an NC-loop in PvEH3, four single-site variants of pveh3 were amplified by PCR, and intracellularly expressed in E. coli BL21(DE3), respectively. E. coli/pveh3E134K and /pveh3T137P had the enhanced EH activities of 15.3 ± 0.4 and 16.1 ± 0.5 U/g wet cell as well as E values of 21.7 ± 1.0 and 21.2 ± 1.1 towards rac-3a. Subsequently, E. coli/pveh3E134K/T137P harboring a double-site variant gene was also constructed, having the highest EH activity of 22.4 ± 0.6 U/g wet cell and E value of 24.1 ± 1.2. The specific activity of the purified PvEH3E134K/T137P (14.5 ± 0.5 U/mg protein) towards rac-3a and its catalytic efficiency (kcat/Km of 5.67 mM−1 s−1) for (S)-3a were 1.7- and 3.54-fold those (8.4 ± 0.3 U/mg and 1.60 mM−1 s−1) of PvEH3. The gram-scale kinetic resolution of rac-3a using whole wet cells of E. coli/pveh3E134K/T137P was performed at 20 °C for 7.0 h, producing (R)-3a with 99.4% ees and 38.5 ± 1.2% yield. Additionally, the mechanism of PvEH3E134K/T137P with remarkably improved E value was analyzed by molecular docking simulation.
Visualization of aquaionic splitting via iron corrosion
Authors: Murakami Shuntaro , Zhang Lihua , Watanabe Seiichi 
Published: February 2020

AbstractWe report a water decomposition mode called ‘Aquaionic Splitting (AiS)’ by means of iron corrosion in aqueous solution. In this paper, we investigated the phenomenon by controlling the reaction between iron and water. A pseudo-sacrificial protection method with oil paint was employed to select the anode and cathode formation locations that govern iron corrosion. Then, the AiS reaction was visualized by using BTB solution, whose colour corresponds to pH, to produce colour patterning that corresponds to the aquaion distribution. It has become clear that water can be selectively separated into protons and hydroxide ions by corrosion control treatment. In this vein, the diffusion coefficient of protons was estimated by using the colour patterning of BTB solution that accompanies iron corrosion, and aquaion distribution was then computer simulated by solving the diffusion equation.
Damage accumulation mechanism in PIN diode limiters induced via multiple microwave pulses
Authors: Zhao Jingtao , Chen Quanyou , Zhao Gang , Chen Chaoyang , Chen Zhidong 
Published: February 2020

AbstractPositive-intrinsic-negative (PIN) diodes are widely used as limiters to protect sensitive components from damage in radio frequency (RF) receiver systems and communication front-ends. However, PIN diode limiters can be burnt out due to the microwave pulses coupling through the front-end of RF receiver systems. The damage processes and mechanisms in PIN limiters are undoubtedly important topics. Here, the damage accumulation process in PIN limiters induced by external microwave pulses is studied via the injection experiments. The relationship between the degree of damage (i.e., insertion loss) in the limiters and number of the injected pulses is reported. The maximum temperature criterion for burnout in PIN limiters is theoretically predicted and experimentally verified not accurate, and it is further observed that the insertion loss of the PIN diode limiter changes significantly only if more energy is injected into the limiter via microwave pulses.
Impact of concurrent right ventricular myocardial infarction on outcomes among patients with left ventricular myocardial infarction
Authors: Liao Huocheng , Chen Qiuyue , Liu Lin , Zhong Sigan , Deng Huazhao , Xiao Chun 
Published: February 2020

AbstractTo compare in-hospital outcomes between left ventricular myocardial infarction (LVMI) patients with and without right ventricular myocardial infarction (RVMI). Patients with acute ST-segment elevation MI (STEMI) undergoing primary percutaneous coronary intervention (PCI) were enrolled and divided into LVMI with and without RVMI groups. Between-group differences and in-hospital outcomes were compared. Compared to patients without RVMI, patients with RVMI were more likely to be male, have higher body mass index, serum levels of C-reactive protein (8.9 ± 2.4 vs 6.2 ± 2.1 mg/dL), B-type natriuretic peptide (1295 ± 340 vs 872 ± 166 pg/mL) and cardiac troponin-I (8.6 ± 2.9 vs 5.2 ± 2.1 ng/mL), and have diabetes (36.3% vs 3.4%) and dyslipidemia (53.4% vs 48.1%). Patients with RVMI had lower left and right ventricular ejection fraction (50.5 ± 5.6% vs 53.4 ± 3.8% and 33.6 ± 2.9% vs 45.7 ± 2.0%), but had higher mean pulmonary artery pressure (30.6 ± 3.3 vs 23.8 ± 3.1 mm Hg). Compared to patients without RVMI, patients with RVMI had higher odds of in-hospital all-cause mortality (4.1% vs 1.0%) and new onset acute heart failure (3.4% vs 1.0%). After adjusted for confounding factors, LVMI with RVMI remained independently associated with composite outcomes, with odds ratio 1.66 (95% confidence interval 1.39–2.04). Compared to isolated LVMI patients, those with concomitant RVMI have higher odds of in-hospital complications, particularly all-cause mortality and new onset acute heart failure.
Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR
Authors: Galicia-Garcia Unai , Benito-Vicente Asier , Uribe Kepa B. , Jebari Shifa , Larrea-Sebal Asier , Alonso-Estrada Rocio , Aguilo-Arce Joseba , Ostolaza Helena , Palacios Lourdes , Martin Cesar 
Published: February 2020

AbstractThe primary genetic cause of familial hypercholesterolemia (FH) is related to mutations in the LDLR gene encoding the Low-density Lipoprotein Receptor. LDLR structure is organized in 5 different domains, including an EGF-precursor homology domain that plays a pivotal role in lipoprotein release and receptor recycling. Mutations in this domain constitute 51.7% of the total missense variants described in LDLR. The aim of the present work was to analyse how clinically significant variants in the EGF-precursor homology domain impact LDLR. The activity of sixteen LDLR variants was functionally characterized by determining LDLR expression by Western blot and LDLR expression, LDL binding capacity and uptake, and LDLR recycling activity by flow cytometry in transfected CHO-ldlA7 cells. Of the analysed variants, we found six non-pathogenic LDLR variants and ten pathogenic variants distributed as follow: three class 3 variants; four class 2 variants; and three class 5 variants. These results can be incorporated into clinical management of patients by helping guide the appropriate level of treatment intensity depending on the extent of loss of LDLR activity. This data can also contribute to cascade-screening for pathogenic FH variants.
CoCrMo surface modifications affect biocompatibility, adhesion, and inflammation in human osteoblasts
Authors: Lohberger Birgit , Stuendl Nicole , Glaenzer Dietmar , Rinner Beate , Donohue Nicholas , Lichtenegger Helga C. , Ploszczanski Leon , Leithner Andreas 
Published: February 2020

AbstractIn this study, different surface modifications were performed on a Cobalt-Chrome-Molybdenum (CoCrMo) alloy and the effects on cell viability and cytotoxicity as well as the adhesion potential of human osteoblasts (hFOB) and their inflammation reaction were investigated in vitro. CoCrMo discs were coated with TiN, with polished and porous coated surfaces, or with pure titanum (cpTi) surfaces and examined by Scanning Electron Microscopy to evaluate surface modifications. In vitro cell viability, adhesion behaviour, and expression of inflammation markers of hFOB human osteoblasts were measured via CellTiter-Glo, CytoTox, ELISA, and RT-PCR respectively. All results were compared to CoCrMo without surface modifications. The biocompatibility data showed high compatibility for the TiN hard coatings. Likewise, the porous surface coating increased cell viability significantly, compared to an untreated CoCrMo alloy. None of the investigated materials influenced cytotoxicity. Different surface modifications did not influence expression of fibronectin, although TiN, porous surface coatings and polished surfaces showed highly significant reductions in integrin subunit expression. In addition to the regulation of adhesion potential these three surfaces stimulated an anti-inflammatory response by osteocytes. Improved biocompatibility and adhesion properties may contribute to better osteointegration of prosthetics.
E-Zigaretten – Funktionsweise, Inhaltsstoffe und die Vaping-assoziierte akute Lungenschädigung
Authors: Schupp J. C. , Prasse A. , Erythropel H. C. 
Published: February 2020

ZusammenfassungSeit April 2019 kam es in den USA zu über 2000 Erkrankungen, bei denen zumeist junge Patienten mit schweren Lungenschädigungen in Krankenhäuser eingewiesen wurden. Im Verlauf sind bisher mehr als 40 Patienten verstorben. Nur ein einziger Zusammenhang konnte zwischen diesen Patienten ermittelt werden: Sie waren alle Nutzer von elektronischen Zigaretten (E-Zigaretten). Mittlerweile wird das Krankheitsbild im Englischen als EVALI bezeichnet (engl. „e-cigarette, or vaping, product use associated lung injury”, E-Zigaretten- oder Vaping-assoziierte Lungenschädigung). Basierend auf zumeist US-amerikanischen Studien zum Thema soll diese Übersichtsarbeit einen Einblick in die Definition, die Epidemiologie, das Krankheitsbild, den typischen Verlauf und potenzielle Auslöser, wie z. B. Vitamin-E-Acetat, von EVALI geben. Zusätzlich soll ein allgemeiner Überblick über die prinzipielle Funktionsweise von E-Zigaretten, die Inhaltsstoffe der verdampften Flüssigkeiten (E-Liquids) sowie die damit verbundenen Inhalationsrisiken gegeben werden.
Ketamine Infusion for Super Refractory Status Epilepticus in Alternating Hemiplegia of Childhood
Authors: Samanta Debopam 
Published: February 2020

AbstractAlternating hemiplegia of childhood (AHC) is a rare neurodevelopmental disorder and characterized by infantile onset hemiplegia involving either side of the body and other paroxysmal spells, including epilepsy. The N-methyl-D-aspartate (NMDA) receptor noncompetitive antagonist ketamine has been proved to be effective terminating status epilepticus (SE) in animal models and human case studies. Less than 50 cases, those use ketamine, have been reported in the management of pediatric SE, but its effectiveness is unknown in patients with AHC. The author reported two patients with molecularly confirmed AHC who presented with super refractory status epilepticus. These two patients failed multiple antiepileptic drugs, including midazolam infusion and propofol but responded promptly to ketamine infusion. This is the first description of the effectiveness of ketamine as an effective third-line agent for super refractory SE associated with AHC. Increased glutamate-mediated neurotoxicity can be present in AHC, not only due to overexpression of glutamate receptors during status epilepticus but also due to unique characteristics of AHC-preferential inactivity of GABAergic interneuron and impaired action of glutamate transporters (EAAT-1). Moreover, as neuroregression is possible after SE in AHC, earlier termination of SE by using ketamine can be an effective option in this population. Further prospective, controlled studies are needed to confirm the finding of this study.
Exosomes derived from HeLa cells break down vascular integrity by triggering endoplasmic reticulum stress in endothelial cells
Authors: Lin Yinuo , Zhang Chi , Xiang Pingping , Shen Jian , Sun Weijian , Yu Hong 
Published: February 2020

ABSTRACTExosomes play a critical role in intercellular communication since they contain signalling molecules and genetic materials. During tumorigenesis, tumour‐derived exosomes have been demonstrated to promote tumour angiogenesis and metastasis. However, how the exosomes facilitate tumour metastasis is not clear. Here we explored the effect of HeLa cell‐derived exosomes (ExoHeLa) on endothelial tight junctions (TJ) and the related mechanisms. After human umbilical vein endothelial cells (HUVEC) were treated with ExoHeLa, TJ proteins zonula occludens‐1 (ZO‐1) and Claudin‐5 in HUVEC were significantly reduced as compared with that treated with exosomes from human cervical epithelial cells, while mRNA levels of ZO‐1 and Claudin‐5 remained unchanged. Consequently, permeability of endothelial monolayer was increased after the treatment with ExoHeLa. Injection of ExoHeLa into mice also increased vascular permeability and tumour metastasis in vivo. Neither knocking down of Dicer nor use of inhibitors of microRNAs targeting at mRNAs of ZO‐1 and Claudin‐5 could block the inhibitory effect of ExoHeLa on ZO‐1 and Claudin‐5. The expression of genes involved in endoplasmic reticulum (ER) stress was significantly increased in HUVECs after treated with ExoHeLa. Inhibition of ER stress by knocking down protein kinase RNA‐like endoplasmic reticulum kinase prevented the down‐regulation of ZO‐1 and Claudin‐5 by ExoHeLa. Our study found that HeLa cell‐derived exosomes promote metastasis by triggering ER stress in endothelial cells and break down endothelial integrity. Such effect of exosomes is microRNA‐independent.
High-frequency spin transfer nano-oscillator based on the motion of skyrmions in an annular groove
Authors: Jin Chendong , Ma Yunxu , Song Chengkun , Xia Haiyan , Wang Jianing , Zhang Chunlei , Zeng Zhaozhuo , Wang Jianbo , Liu Qingfang 
Published: February 2020

Abstract
Magnetic skyrmion-based spin transfer nano-oscillators (STNOs) have been proposed as microwave signal generators and attracted enormous interest recently. However, the oscillation frequency of skyrmion-based STNOs is about 2 GHz, which is not so high for practical applications. In this paper, we create an annular groove in the surface of the free layer and put skyrmions in the annular groove. Due to the potential of the groove, skyrmions are confined to moving in the groove when driven by the spin-polarized currents. Through micromagnetic simulations, it is found that the frequency tunability of the STNO with the presence of the annular groove reaches to 15.63 GHz, which is more than 6 times higher than the case without the presence of the annular groove because of three reasons: the oscillation radius of skyrmions can be adjusted by the groove, the potential of the groove is larger than that of the edge and the groove can limit the diameter of skyrmions so that a larger number of skyrmions can be placed in the groove. Our results present the understanding of dynamic of skyrmions in an annular groove, which provides alternative possibilities for the design of skyrmion-based STNOs.
Non-Markovian dynamics of a quantum heat engine: out-of-equilibrium operation and thermal coupling control
Authors: Wiedmann M , Stockburger J T , Ankerhold J 
Published: February 2020

Abstract
Real quantum heat engines lack the separation of time and length scales that is characteristic for classical engines. They must be understood as open quantum systems in non-equilibrium with time-controlled coupling to thermal reservoirs as integral part. Here, we present a systematic approach to describe a broad class of engines and protocols beyond conventional weak coupling treatments starting from a microscopic modeling. For the four stroke Otto engine the full dynamical range down to low temperatures is explored and the crucial role of the work associated with the coupling/de-coupling to/from reservoirs as an integral part in the energy balance is revealed. Quantum correlations turn out to be instrumental to enhance the efficiency which opens new ways for optimal control techniques.
Response Surface Optimization of Essential Oils Production from Clove Leaf Waste by Microwave-Assisted Hydro Distillation
Authors: Astuti Handayani Prima , Witri Sofiarani Anisa , Bagas Kurniawan Aditya 
Published: February 2020

Abstract
Essential oils can be obtained from leaves, flowers, stems, and roots. One of the ingredients that has the potential to become essential oils is clove plants. The content of essential oils in clove leaves is between 1-4%. The process of refining essential oils can use the microwave assisted hydrodistillation method. The use of microwaves is to speed up processing time. Optimization using the Response Surface Methodology (RSM) method is based on Central Composite Design (CCD). The research variables of micro wave power are 400-800 watt and clove leaf mass 70-90 gram. The optimization results obtained at 599.5 watts of microwave power and clove leaf mass of 65.86 grams with a yield of 1.1835%. The second order polynomial equation is obtained y = 0.827502 + 0.493345X1 - 0.179890X2 – 0.3363901X1
2 + 0.228974X2
2 + 0.227850X1X2.