bluuebunny/crossref_metadata_2025_split huggingface dataset:
Numerical analysis of thermal conductive hybrid nanofluid flow over the surface of a wavy spinning disk
Authors: Ahmadian Ali , Bilal Muhammad , Khan Muhammad Altaf , Asjad Muhammad Imran 
Published: November 2020

AbstractA three dimensional (3D) numerical solution of unsteady, Ag-MgO hybrid nanoliquid flow with heat and mass transmission caused by upward/downward moving of wavy spinning disk has been scrutinized. The magnetic field has been also considered. The hybrid nanoliquid has been synthesized in the presence of Ag-MgO nanoparticles. The purpose of the study is to improve the rate of thermal energy transmission for several industrial purposes. The wavy rotating surface increases the heat transmission rate up to 15%, comparatively to the flat surface. The subsequent arrangement of modeled equations is diminished into dimensionless differential equation. The obtained system of equations is further analytically expounded via Homotopy analysis method HAM and the numerical Parametric continuation method (PCM) method has been used for the comparison of the outcomes. The results are graphically presented and discussed. It has been presumed that the geometry of spinning disk positively affects the velocity and thermal energy transmission. The addition of hybrid nanoparticles (silver and magnesium-oxide) significantly improved thermal property of carrier fluid. It uses is more efficacious to overcome low energy transmission. Such as, it provides improvement in thermal performance of carrier fluid, which play important role in power generation, hyperthermia, micro fabrication, air conditioning and metallurgical field.
Signatures of natural selection and ethnic-specific prevalence of NPC1 pathogenic mutations contributing to obesity and Niemann–Pick disease type C1
Authors: Chiorean Andreea , Garver William S. , Meyre David 
Published: November 2020

AbstractWhile homozygous pathogenic mutations in the NPC1 gene cause Niemann-Pick type C1 disease, heterozygous mutations cause highly-penetrant obesity. We aimed to investigate the prevalence of NPC1 mutations and their signatures of natural selection in 122,678 exome sequenced participants from six ethnic groups in the Genome Aggregation Database. Pathogenic missense coding mutations were identified by in silico tools and the ClinVar database. Signatures of natural selection were assessed by the probability of NPC1 being loss-of-function mutation intolerant and Z-scores of observed/expected synonymous and non-synonymous mutation ratios. There was no evidence of negative selection observed for synonymous, non-synonymous and loss-of-function mutations. However, there were significant ethnic differences in the prevalence of heterozygous pathogenic NPC1 mutations ranging from 0.56% in Ashkenazi Jewish to 3.26% in African/African Americans (5.8-fold difference). Four homozygous carriers of pathogenic NPC1 mutations were also identified, belonging to the South Asian population. In conclusion, NPC1 mutations are consistent with a model of balanced selection, where heterozygotes and homozygotes have higher and lower reproductive fitness, respectively. Therefore, NPC1 heterozygous mutations may account for a substantial and ethnic-dependent percentage of obesity in the general population, while NPC1 homozygous mutations may be frequent in the South Asian populations and warrants more investigation.
Mass spectrometric quantitation of AGEs and enzymatic crosslinks in human cancellous bone
Authors: Arakawa Shoutaro , Suzuki Ryusuke , Kurosaka Daisaburo , Ikeda Ryo , Hayashi Hiroteru , Kayama Tomohiro , Ohno Rei-ichi , Nagai Ryoji , Marumo Keishi , Saito Mitsuru 
Published: November 2020

AbstractAdvanced glycation end-products (AGEs) deteriorate bone strength. Among over 40 species identified in vivo, AGEs other than pentosidine were roughly estimated as total fluorescent AGEs (tfAGEs) due to technical difficulties. Using LC-QqTOF-MS, we established a system that enabled the quantitation of five AGEs (CML, CEL, MG-H1, CMA and pentosidine) as well as two mature and three immature enzymatic crosslinks. Human bone samples were collected from 149 patients who underwent total knee arthroplasty. Their clinical parameters were collected to investigate parameters that may be predictive of AGE accumulation. All the analytes were quantitated and showed significant linearity with high sensitivity and precision. The results showed that MG-H1 was the most abundant AGE, whereas pentosidine was 1/200–1/20-fold less abundant than the other four AGEs. The AGEs were significantly and strongly correlated with pentosidine, while showing moderate correlation with tfAGEs. Interestingly, multiple linear regression analysis revealed that gender contributed most to the accumulation of all the AGEs, followed by age, tartrate-resistant acid phosphatase-5b and HbA1c. Furthermore, the AGEs were negatively correlated with immature crosslinks. Mass spectrometric quantitation of AGEs and enzymatic crosslinks is crucial to a better understanding of ageing- and disease-related deterioration of bone strength.
The magnitude and extent of edge effects on vascular epiphytes across the Brazilian Atlantic Forest
Authors: Parra-Sanchez Edicson , Banks-Leite Cristina 
Published: November 2020

AbstractEdge effects are ubiquitous landscape processes influencing over 70% of forest cover worldwide. However, little is known about how edge effects influence the vertical stratification of communities in forest fragments. We combined a spatially implicit and a spatially explicit approach to quantify the magnitude and extent of edge effects on canopy and understorey epiphytic plants in the Brazilian Atlantic Forest. Within the human-modified landscape, species richness, species abundance and community composition remained practically unchanged along the interior-edge gradient, pointing to severe biotic homogenisation at all strata. This is because the extent of edge effects reached at least 500 m, potentially leaving just 0.24% of the studied landscape unaffected by edges. We extrapolated our findings to the entire Atlantic Forest and found that just 19.4% of the total existing area is likely unaffected by edge effects and provide suitable habitat conditions for forest-dependent epiphytes. Our results suggest that the resources provided by the current forest cover might be insufficient to support the future of epiphyte communities. Preserving large continuous ‘intact’ forests is probably the only effective conservation strategy for vascular epiphytes.
Protective effects of tryptophan-catabolizing Lactobacillus plantarum KLDS 1.0386 against dextran sodium sulfate-induced colitis in mice
Authors: Shi Jialu , Du Peng , Xie Qinggang , Wang Nana , Li Huizhen , Smith Etareri Evivie , Li Chun , Liu Fei , Huo Guicheng , Li Bailiang 
Published: November 2020

L. plantarum KLDS 1.0386 combined with tryptophan alleviates ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) by increasing the level of indole-3-acetic acid (IAA), stimulating the AHR/IL-22/STAT3 signaling pathway and regulating gut microbiota in mice.
Experimental investigation of the low-frequency impact sound transfer function of lightweight wooden floors via heel drops
Authors: Qin Yi , Tan Jin Jack , Hornikx Maarten 
Published: November 2020

The lightweight floor is a popular construction choice in buildings despite its poor low-frequency impact sound performance. This is exacerbated by common human activities, such as walking and jumping, that have high input force levels at low frequencies. Therefore, experimental evaluations of the low-frequency impact sound performance of a wooden lightweight floor are of interest to designers and researchers. The aim of this paper is to explore the use of heel drop for impact sound transfer measurement. An impact force plate has been built to accurately measure the heel-drop forces up to 200 Hz and the performances on two types of floors are evaluated. The heel drop has a higher energy level at low frequencies, resulting in higher coherence and signal-to-noise ratio and hence superior performance in characterizing the floors in the frequency range of below 40 Hz when compared to the impact hammer. Interestingly, in the case of a heel-drop excitation, the first natural frequencies of the floor decrease. It is in contrast to when a human is simply standing on it, which resulted in an increase of the natural frequencies upon impact hammer excitation.
Nonoperative Management for T2 Low Rectal Cancer: A Western Approach
Authors: Fernandez Laura Melina , São Julião Guilherme Pagin , Vailati Bruna Borba , Habr-Gama Angelita , Perez Rodrigo Oliva 
Published: November 2020

AbstractThe possibility of organ preservation in early rectal cancer has gained popularity during recent years. Patients with early tumor stage and low risk for local recurrence do not usually require neoadjuvant chemoradiation for oncological reasons. However, these patients may be considered for chemoradiation exclusively for the purpose of achieving a complete clinical response and avoid total mesorectal excision. In addition, cT2 tumors may be more likely to develop complete response to neoadjuvant therapy and may constitute ideal candidates for organ-preserving strategies. In the setting where the use of chemoradiation is exclusively used to avoid major surgery, one should consider maximizing tumor response. In this article, we will focus on the rationale, indications, and outcomes of patients with early rectal cancer being treated by neoadjuvant chemoradiation to achieve organ preservation by avoiding total mesorectal excision.
Accurate and efficient calculation of photoionization in streamer discharges using fast multipole method
Authors: Lin Bo , Zhuang Chijie , Cai Zhenning , Zeng Rong , Bao Weizhu 
Published: November 2020

Abstract
This paper focuses on the three-dimensional simulation of the photoionization in streamer discharges, and provides a general framework to efficiently and accurately calculate the photoionization model using the integral form. The simulation is based on the kernel-independent fast multipole method (FMM). The accuracy of this method is studied quantitatively for different domains and various pressures in comparison with other existing models based on partial differential equations (PDEs). The comparison indicates the numerical error of the FMM is much smaller than those of other PDE-based methods, with the reference solution given by direct numerical integration. Such accuracy can be achieved with affordable computational cost, and its performance in both efficiency and accuracy is quite stable for different domains and pressures. Meanwhile, the simulation accelerated by the FMM exhibits good scalability using up to 1280 cores, which shows its capability of three-dimensional simulations using parallel (distributed) computing. The difference of the proposed method and other efficient approximations are also studied in a three-dimensional dynamic problem where two streamers interact.
Monitoring oxidative metabolism while modeling pancreatic ischemia in mice using a multimodal spectroscopy technique
Authors: Dremin Viktor , Potapova Elena , Mamoshin Andrian , Dunaev Andrey , Rafailov Edik 
Published: November 2020

Abstract
This paper describes the experimental monitoring of pancreatic oxidative metabolism in laboratory mice that combines the methods of fluorescence and diffuse reflectance spectroscopy and laser speckle contrast imaging with a pancreatic ischemia model. The combined measurements show a close dependence of tissue metabolism on blood and oxygen supply. We show that deactivation of complex I and complex II occurs in mouse pancreatic tissue during prolonged hypoxia. We conclude that complex I can potentially undergo more intensive deactivation when oxygen is lacking than complex II. We have demonstrated that the methods described can be applied in minimally invasive surgery of the pancreas to assess its viability.
Heat Stress and Cardiac Strain in French Vineyard Workers
Authors: Grimbuhler Sonia , Viel Jean-François 
Published: November 2020

Abstract
Agricultural workers often produce considerable excess heat due to the physically demanding nature of their activities, increasing their risk of thermal stress in even moderately warm conditions. Few studies have examined the physiological responses to heat load in agriculture. We aimed to assess the heat strain experienced by vineyard workers during canopy management in dry field conditions, and to disentangle the effects of the heat produced by the body and the thermal environment. Thirty workers from five Bordeaux vineyards of southern France were monitored during vine-lifting and trellising (June 2012). The mean heart rate, net cardiac cost, relative cardiac cost, and cardiac workload score were assessed during field activity. As the workers were nested within vineyards, multilevel linear regression models were used for correct inference. Skin temperature increased by an average of 1.0°C. Cardiac indices showed marked differences between individuals. The workload was evaluated as ‘heavy’ or ‘very heavy’ for more than one-third of the workers, of whom one experienced heat exhaustion. Above some individual characteristics, we highlighted a contextual effect (air temperature) for the mean heart rate (P = 0.03), the relative cardiac cost (P = 0.01) and, to a lesser extent, a cardiac workload score (P = 0.07). Canopy management by hand in vineyards causes considerable cardiac and thermoregulatory strain. Appropriate instruments should be developed to simultaneously evaluate work intensity, work quality, and productivity at the vineyard level to raise the awareness of both managers and employees about taking preventive measures.
Occupational Exposure to Blood-Borne Pathogens among Healthcare Workers in a Tertiary Care Center in Lebanon
Authors: Sakr Carine J , Alameddine Reina , Hoteit Reem , Daou Carla , Doudakian Rita , Kreidieh Khalil , Musharrafieh Umayya , Rahme Diana V 
Published: November 2020

Abstract

Objectives
Despite numerous initiatives, occupational exposure to blood-borne pathogens (BBP) caused by percutaneous injuries or mucosal contamination remain common among healthcare workers (HCWs). These exposures were decreasing at the American University of Beirut Medical Center (AUBMC) in the previous decades. Recently, the medical center activity has been increasing with higher number of interventions performed and shorter hospital stay. Our aim was to determine the trend of incidents resulting from BBP exposures at AUBMC from 2014 till 2018 and identify whether the increase in hospital activity affected the rate of these exposures. We also aimed to assess the risk factors associated with needle stick injuries (NSIs).

Methods
A retrospective observational descriptive study of all exposures to BBPs among HCWs reported to the Environmental Health, Safety, and Risk Management department at the AUBMC between 2014 and 2018 was performed.

Results
There were 967 exposures reported among which 84% were due to needlesticks. Residents (40%), followed by nurses (30%), and then by attending physicians (16%) were the top three most exposed occupational groups. Half of the participants injured themselves using either a syringe or a suture needle; and mostly during or after use. Occupation and incident location were associated with NSIs. The mean BBP exposure incidence rate was 5.4 per 100 full-time employees, 65.6 per 100 bed-years, and 0.48 admission-years. The BBP exposure rate per 100 occupied beds per year decreased between 2014 and 2017 then increased in 2018 (P &lt; 0.001). The number of BBP exposures showed a strong, though non-significant negative correlation with the average length of hospital stay (Spearman correlation coefficient = −0.9, P = 0.083).

Conclusions
BBP exposure remains a serious occupational hazard. Our study shows that the BBP exposure rate per 100 occupied beds per year started decreasing during the study period before increasing again in 2018. Only the nursing department showed a consistent decrease of exposures. The occupation and incident location were found to be risk factors associated with NSIs. In addition to providing education and training, additional steps such as providing safety equipment and future interventions directed towards adjusting to higher workload should be all considered.
Mutant p53 oncogenicity: dominant-negative or gain-of-function?
Authors: Stein Yan , Aloni-Grinstein Ronit , Rotter Varda 
Published: November 2020

Abstract
The p53 protein is mutated in about 50% of human cancers. Aside from losing its tumor-suppressive activities, mutant p53 may acquire pro-oncogenic activity, which is facilitated by two underlying mechanisms. The first mechanism is the inhibition of co-expressed wild-type p53 (WTp53) activity, dubbed the dominant-negative effect (DNE). The second mechanism is a neomorphic pro-oncogenic activity that does not involve the inhibition of WTp53, termed gain-of-function (GOF). Throughout the years, both mechanisms were demonstrated in a plethora of in vitro and in vivo models. However, whether both account for protumorigenic activities of mutant p53 and in which contexts is still a matter of ongoing debate. Here, we discuss evidence for both DNE and GOF in a variety of models. These models suggest that both GOF and DNE can be relevant, but are highly dependent on the specific mutation type, genetic and cellular context and even the phenotype that is being assessed. In addition, we discuss how mutant and WTp53 might not exist as two separate entities, but rather as a continuum that may involve a balance between the two forms in the same cells, which could be tilted by various factors and drugs. Further elucidation of the factors that dictate the balance between the WT and mutant p53 states, as well as the factors that govern the impact of DNE and GOF in different cancer types, may lead to the development of more effective treatment regimens for cancer patients.
Beyond the 405 and the 5: Geographic Variations and Factors Associated With Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Positivity Rates in Los Angeles County
Authors: Vijayan Tara , Shin Michael , Adamson Paul C , Harris Christina , Seeman Teresa , Norris Keith C , Goodman-Meza David 
Published: November 2020

Abstract

Background
To highlight geographic differences and the socio-structural determinants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) test positivity within Los Angeles County (LAC).

Methods
A geographic information system was used to integrate, map, and analyze SARS-CoV-2 testing data reported by the LAC Department of Public Health and data from the American Community Survey. Structural determinants included race/ethnicity, poverty, insurance status, education, and population and household density. We examined which factors were associated with positivity rates, using a 5% test positivity threshold, with spatial analysis and spatial regression.

Results
Between 1 March and 30 June 2020 there were 843 440 SARS-CoV-2 tests and 86 383 diagnoses reported, for an overall positivity rate of 10.2% within the study area. Communities with high proportions of Latino/a residents, those living below the federal poverty line, and with high household densities had higher crude positivity rates. Age- adjusted diagnosis rates were significantly associated with the proportion of Latino/as, individuals living below the poverty line, and population and household density.

Conclusions
There are significant local variations in test positivity within LAC and several socio-structural determinants contribute to ongoing disparities. Public health interventions, beyond shelter in place, are needed to address and target such disparities.
Neural Antibody Testing in Patients with Suspected Autoimmune Encephalitis
Authors: Budhram Adrian , Dubey Divyanshu , Sechi Elia , Flanagan Eoin P , Yang Liju , Bhayana Vipin , McKeon Andrew , Pittock Sean J , Mills John R 
Published: November 2020

AbstractBackgroundAutoimmunity is an increasingly recognized cause of encephalitis with a similar prevalence to that of infectious etiologies. Over the past decade there has been a rapidly expanding list of antibody biomarker discoveries that have aided in the identification and characterization of autoimmune encephalitis. As the number of antibody biomarkers transitioning from the research setting into clinical laboratories has accelerated, so has the demand and complexity of panel-based testing. Clinical laboratories are increasingly involved in discussions related to test utilization and providing guidance on which testing methodologies provide the best clinical performance.ContentTo ensure optimal clinical sensitivity and specificity, comprehensive panel-based reflexive testing based on the predominant neurological phenotypic presentation (e.g., encephalopathy) is ideal in the workup of cases of suspected autoimmune neurological disease. Predictive scores based on the clinical workup can aid in deciding when to order a test. Testing of both CSF and serum is recommended with few exceptions. Appropriate test ordering and interpretation requires an understanding of both testing methodologies and performance of antibody testing in different specimen types.SummaryThis review discusses important considerations in the design and selection of neural antibody testing methodologies and panels. Increased collaboration between pathologists, laboratorians, and neurologists will lead to improved utilization of complex autoimmune neurology antibody testing panels.
Warmer temperatures interact with salinity to weaken physiological facilitation to stress in freshwater fishes
Authors: Walker Richard H , Smith Geoffrey D , Hudson Spencer B , French Susannah S , Walters Annika W 
Published: November 2020

AbstractManagement of stressors requires an understanding of how multiple stressors interact, how different species respond to those interactions and the underlying mechanisms driving observed patterns in species’ responses. Salinization and rising temperatures are two pertinent stressors predicted to intensify in freshwater ecosystems, posing concern for how susceptible organisms achieve and maintain homeostasis (i.e. allostasis). Here, glucocorticoid hormones (e.g. cortisol), responsible for mobilizing energy (e.g. glucose) to relevant physiological processes for the duration of stressors, are liable to vary in response to the duration and severity of salinization and temperature rises. With field and laboratory studies, we evaluated how both salinity and temperature influence basal and stress-reactive cortisol and glucose levels in age 1+ mottled sculpin (Cottus bairdii), mountain sucker (Catostomus platyrhynchus) and Colorado River cutthroat trout (Oncorhynchus clarki pleuriticus). We found that temperature generally had the greatest effect on cortisol and glucose concentrations and the effect of salinity was often temperature dependent. We also found that when individuals were chronically exposed to higher salinities, baseline concentrations of cortisol and glucose usually declined as salinity increased. Reductions in baseline concentrations facilitated stronger stress reactivity for cortisol and glucose when exposed to additional stressors, which weakened as temperatures increased. Controlled temperatures near the species’ thermal maxima became the overriding factor regulating fish physiology, resulting in inhibitory responses. With projected increases in freshwater salinization and temperatures, efforts to reduce the negative effects of increasing temperatures (i.e. increased refuge habitats and riparian cover) could moderate the inhibitory effects of temperature-dependent effects of salinization for freshwater fishes.