bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative proteomics reveals stage-specific protein regulation of triple negative breast cancer
Authors: Lin Yuxiang , Lin Ling , Fu Fangmeng , Wang Chuan , Hu Anqi , Xie Juanjuan , Jiang Meichen , Wang Zhenxin , Yang Lujie , Zhang Jie , Chen Minyan , Li Yan , Guo Rongrong , Yang Pengyuan , Shen Huali 
Published: February 2020

Abstract
BackgroundTriple negative breast cancer (TNBC) is a heterogeneous disease with more aggressive clinical courses than other subtypes of breast cancer. In this study, we performed high-resolution mass spectrometry-based quantitative proteomics with TNBC clinical tissue specimens to explore the early and sensitive diagnostic signatures and potential therapeutic targets for TNBC patients. Methods We performed an iTRAQ-labeling coupled LC-MS/MS approach to explore the global proteome in tumor tissues and corresponding para-tumor tissues from 24 patients with grade I-II and grade III primary TNBC. Relative peptide quantification and protein identification were performed by Proteome Discoverer TM software with Mascot search engine. Differentially expressed proteins were analyzed by bioinformatic analyses, including GO function classification annotation and KEGG enrichment analysis. Pathway analyses for protein-protein interactions and upstream regulations of differentially expressed candidates were performed by Ingenuity Pathway Analysis (IPA) software. Results Totally, 5,401 unique proteins were identified and quantified in different stage of TNBCs. 865 proteins were changed in patients with grade I or II TNBC, among which 309 were up-regulated and 556 were down-regulated. Meanwhile, for patients with grade III TNBC, 359 proteins were increased and 672 proteins were decreased. Comparing to para-cancerous tissues, various signaling pathways and metabolic processes, including PPAR pathways, PI3K-Akt pathway, one-carbon metabolism, amino acid synthesis, and lipid metabolism were activated in TNBC cancer tissues. Death receptor signaling was significantly activated in grade I-II TNBCs, however, remarkably inhibited in grade III TNBCs. Conclusions Overall, our proteomic data presented precise quantification of potential signatures, signaling pathways, regulatory networks, and characteristic differences in each clinicopathological subgroup. The proteome provides complementary information for TNBC accurate subtype classification and therapeutic targets research.
Effects of vitamin D supplementation on depression in patients with colorectal cancer
Authors: Duan JianWen , Chen YongSheng , Wu WenFei , Xiong Cong , Hu ZuLiang , chen wenyu , XU Yufen 
Published: February 2020

Abstract
Background: The relation and possible mechnism of vitamin D supplementation on depression in colorectal cancer (CRC) patients was not clearly ;
Objective: This study investigates the effect of vitamin D supplementation on depression in colorectal cancer (CRC) patients.
Methods : We recruited 168 CRC patients and 168 healthy control subjects into this study. 17-item Hamilton Depression Rating Scale (HDRS-17) was used to assess depression.
Results: We found that 25-hydroxyvitamin D (25(OH)D) concentrations were independently associated with depression among CRC patients. For the 45 depressed patients receiving vitamin D3 supplementation, depression scores decreased markedly with 25(OH)D concentrations increasing to normal.
Conclusion: Therefore, we advise monitoring this indicator in CRC patients and supplementing with vitamin D 3 when their 25(OH)D concentrations are low.
Prevalence and Distribution of Resistance and Enterotoxins/Enterotoxin-likes Genes in Different Clinical Isolates of Coagulase-negative Staphylococcus
Authors: Nasaj Mona , Saeidi Zahra , Arabestani Mohammad 
Published: February 2020

Abstract
Background CoNS serve as a major reservoir for genes facilitating the evolution of S. aureus as a successful pathogen. The present study aimed to determine the occurrence of genes conferring resistance to fluoroquinolone, determining of the prevalence of insertion sequence elements IS256 and IS257 and different superantigenes (SAgs) among CoNS isolates obtained from various clinical sources. Materials and Methods The current study conducted on a total of 91 CoNS species isolated from clinical specimens in Hamadan hospitals in western Iran in 2017-19. The Antimicrobial susceptibility testing was performed using disk diffusion method and the presence of the IS256 and IS257, genes conferring resistance to fluoroquinolone and enterotoxins/enterotoxin-likes encoding genes were investigated by PCR method. Results Among genes encoding classic enterotoxins, sec was the most frequent which was carried by 48.4% of isolates, followed by seb in 27.5%. None of the CoNS isolates were found to be positive to enterotoxin-like encoding genes. Among the 11 CoNS isolates that have shown phenotypically resistant to levofloxacin, 9 isolates (81.8%) with gyrB , 8 isolates (72.7%) with gyrA, 8 isolates (72.7%) with grlB and 7 isolates (63.6%) with grlA were identified. The IS256 and IS257 were identified in 31.8% and 74.7% of CoNS isolates. The results of statistical analysis showed a significant association between the occurrence of SEs encoding genes and antimicrobial resistance. Conclusion Antimicrobial resistant determinants and staphylococcus enterotoxins (SEs) are co-present in clinical CoNS isolates that confer selective advantage for colonization and survival in hospital settings. The coexistence of insertion elements and antibiotic resistance indicate their role in pathogenesis and infectious disease.
The influence of various smoking statuses on the resting heart rate in a physical examination population with high-normal blood pressure
Authors: chen wenyu , Fang Zhixian , Xu Yufeng 
Published: February 2020

Abstract
Background The exact relation between smoking statuses and hypertension in the population with high-normal BP is poorly understood. Objective To investigate the influence of various smoking statuses (active smoking, passive smoking, and non-smoking) on the resting heart rate of the population with high-normal blood pressure. Methods This was a cross-sectional study of patients undergoing physical examination at three Chinese hospitals between January 2018 and December 2019, and who conformed with the criteria of high-normal BP. The subjects were classified into the non-smoking, active smoking and passive smoking groups. The resting heart rate (RHR) and blood pressure (BP) were measured. High-density lipoprotein cholesterol (HDL-C), low-density lipoprotein (LDL-C), triglycerides (TG), total cholesterol (TC), and fasting blood glucose (FBG) were measured. Results TG, TC, and FBG were significantly higher in the active smoking group than in the two other groups (all P<0.05). HDL-C levels were lower in the active smoking group than in the two other groups (P<0.05). There were significant differences in RHR among the three groups (active: 76.0±10.1 vs. passive: 72.6±5.8 vs. non-smoking: 69.4±4.4 bpm, P<0.01). Active smoking (odds ratio (OR)=5.13, P<0.05), SBP (OR=0.70, P<0.05), HDL-C (OR=0.58, P<0.05), and passive smoking (OR=3.57, P<0.05) were independently associated with increased RHR. ConclusionRHR was higher in subjects with high-normal BP and in the passive and active smoking groups. Strengthening smoking cessation education and avoiding exposure secondary smoke could be beneficial to control the population with high-normal BP.
Graphene Modified Montmorillonite Based Phase Change Material for Thermal Energy Storage with Enhanced Interfacial Thermal Transfer
Authors: Peng Kang , Wang Hongjie , wan Pengfei , Wang Jianwei , Luo Hua , Zhou Senhai , Li Xiaoyu , Yang Jun 
Published: February 2020

Abstract
Thermal energy storage technology plays a crucial role in the thermal management system. Clay based organic phase change material has considerable advantages in the application of thermal energy storage due to low cost and high energy storage capacity. However, the low thermal conductivity of clay, especially poor interfacial thermal transfer, limits its thermal energy storage efficiency. Herein, stearic acid/reduced graphene oxide modified montmorillonite composites (SA/RGO-MMT) were prepared by the vacuum impregnation of stearic acid into graphene modified montmorillonite matrix, which was obtained via the in situ reduction of graphene oxide on the surface of montmorillonite. Stearic acid is assembled in the porous structures of RGO-MMT with the physical interactions. SA/RGO-MMT possesses high melting enthalpy of 159 J/g, low extent of supercooling of 1.4 oC and excellent thermal reliability after 100 thermal cycling. Energy storage and release rates of SA/RGO-MMT were significantly improved due to the enhanced interfacial thermal transfer by graphene. Therefore, SA/RGO-MMT is a promising form-stable phase change material for applications in solar heat storage fields. The strategy in this study highlights the importance of enhancing interfacial thermal transfer for the efficient thermal energy storage materials.
Proadrenomedullin as a prognostic biomarker for critically-ill septic children
Authors: Ghobrial Emad E. , Khorshied Mervat M , Adly Sally B , Aziz Miriam Magdy 
Published: February 2020

Abstract
Background: The use of new biomarkers is a promising tool to assess risk of mortality among septic children. The aim of the study was to confirm prognostic and diagnostic value of pro-ADM as a marker in critically ill septic children. Methods: This study was conducted on 40 patients who were admitted at pediatric intensive care unit (PICU), diagnosed as sepsis in addition to 40 patients enrolled as control group. Serum pro-ADM level was measured for both cases and controls. Results: The level of pro-ADM was significantly higher in cases than controls (p-value <0.001). However, no correlation between level of pro-ADM and outcome (p value 0.720) was found. Conclusion: The pro-ADM level was higher in cases than control, thus proved its diagnostic value but can’t be used as prognostic factor. Future studies are recommended and daily measurements are warranted to study its prognostic value.
Academic-Service Partnership: Development and Psychometric Evaluation of Achieving Mutual Benefits Scale (AMBS)
Authors: Sadeghnezhad Maliheh , Nabavi Fatemeh Heshmati , Najafi Fereshteh , Kareshki Hossein , Esmaily Habibollah 
Published: February 2020

Abstract
Background The first step for the establishment of academic-service partnership is identification of mutual benefits. The rate of achievement of these benefits reflects the success rate of the partnership. This study aimed develop an achieving mutual benefits scale (AMBS) in nursing academic-service partnership. Method First, the concept of mutual benefits was defined and analyzed by the hybrid model approach. Then, the psychometric properties of the scale were evaluated. Results Exploratory factors analysis supported six factor with an explained variance of 59.06%. The Cronbach’s α, and Omega coefficient of the scale respectively ranged from 0.77 to 0.93, and 0.75 to 0.9. The content validity index of 0.97, and internal consistency of 0.76 were obtained for the final scale. Conclusions The findings showed that mutual benefits have different dimensions in nursing academic-service partnership include nursing education, research and services. It is necessary to consider all dimensions in assessing the success rate of these programs.
Genome-wide association study and Genomic Prediction of spot blotch disease in wheat (Triticum aestivum L.) using genotyping by sequencing
Authors: Tomar Vipin , Singh Ravi P , Poland Jesse , Singh Daljit , Joshi Arun K , Singh Pawan K , Bhati Pradeep K. , Kumar Suneel , Rahman Mokhlesur , Dhillon Guriqbal S , Tiwari Budhi S , Kumar Uttam 
Published: February 2020

Abstract
Background Spot blotch caused by Bipolaris sorokiniana is a major constraint in wheat production in tropics and subtropics. There is limited information available on GWAS and study on genomic prediction is completely lacking. To reveal the genetic markers associated with disease resistance, we performed a genome-wide association study (GWAS) for spot blotch disease in 141 spring wheat lines. Results Based on the testing under natural infection in three years at hot spots location in Pusa, India and Jamalpur, Bangladesh, the genotypes showed significant genetic variation for disease severity. Using Genotyping-by-Sequencing (GBS) based 18637 polymorphic SNP markers and phenotyping from diverse environments, we identified 23 genomic regions across the genome ( p < 0.001) on 14 chromosomes associated with disease scores. Consistent with the previous reports, a most stable genomic region on chromosome 2B, 5B and 7D were detected across the environments. The new genomic region on chromosome 3D was also identified. We performed functional annotation with wheat genome assembly annotation (IWGSC Ref Seq v1.0) and identified NBS-LRR and 35 other plant defense-related protein families across multiple chromosome regions. Using a five-fold cross-validation scheme, we observed moderate prediction accuracy for 3 of 4 environments indicated that our model was able to successfully capture the quantitative variation underlying the SB variation in our population. Conclusions The GWAS based on the phenotypic data from PUSA India and BARI Bangladesh resulted in a total of 23 genomic regions on 14 chromosomes. The new genomic region appeared on chromosome 3D associated with Zinc finger protein that play important role in plant disease resistance. The genomic prediction model for spot blotch disease resistance in wheat was tested and obtained moderate prediction accuracy.
Localized environmental heterogeneity drives the population differentiation of two endangered and endemic Opisthopappus Shih species
Authors: Ye Hang , Wang Zhi , Hou Huimin , Wu Jiahui , Gao Yue , Han Wei , Ru Wenming , Sun Genlou , Wang Yiling 
Published: February 2020

Abstract
Background: Climate heterogeneity not only indirectly shapes the genetic structures of plant populations, but also drives adaptive divergence by impacting demographic dynamics. The variable localized climate and topographic complexity of the Taihang Mountains make them a major natural boundary in Northern China that influences the divergence of organisms distributed in this region. Opisthopappus is an endemic genus of the Taihang Mountains that includes only two spatially partitioned species Opisthopappus longilobus and Opisthopappus taihangensis. For this study, potential major indicators were investigated to explore those associated with the genetic variations in Opisthopappus populations. Results: The findings revealed that a significant genetic differentiation existed between O. longilobus and O. taihangensis populations. All identified haplotypes/studied populations were divided into two distinct groups. The considerable variability was due not only to geographic factors, but also environmental variables. At approximately 13.2 Ma of the Middle Miocene, O. taihangensis differentiated from O. longilobus under a strengthening East Asian monsoon and global cooling. Subsequently, with the uplift of the Taihang Mountains, the complex topography further promoted the variation between the two species and populations. Among environmental factors, five climatic variables, isothermality (bio3), min temperature of coldest month (bio6), mean temperature of wettest quarter (bio8), precipitation of driest month (bio14) and precipitation of wettest quarter (bio16) were demonstrated to be critical toward shaping the genetic components and genetic differentiation of the Opisthopappus species. Moreover, bio6 and bio14 had more driving force than others.Conclusions: The temperature and precipitation of the Taihang Mountains during winter are the key environmental indicators responsible for the current genetic distribution. Our study revealed the importance of localized climatic events by taking the small-scale spatial effects of climate heterogeneity and its impacts on genetic variation into account.
Needs, Aggravation, and Degree of Burnout in Informal Care Providers of Patients with Chronic CVD
Authors: Szlenk-Czyczerska Elżbieta , Guzik Marika , Bielska Dorota Emilia , Ławnik Anna , Polański Piotr , Kurpas Donata 
Published: February 2020

Abstract
Background. A growing number of patients with chronic cardiovascular disease (CVD) creates a growing demand for homecare. Personal and professional limitations, health issues, and lack of systemic support of informal care providers are major causes of insufficient care models for chronically ill patients in home environments. This study aimed to identify the sociodemographic variables that are associated with the needs and increasing occupational burnout observed among home care providers.Methods. This study reports on 161 informal home care providers of patients with CVDs. The research was conducted in the homes of patients, using the Camberwell Assessment of Need Short Appraisal Schedule (CANSAS), the Maslach Burnout Inventory (MBI), and a structured interview questionnaire developed by the authors. Spearman’s rank correlation coefficient test and logistic regression were used for analyses.Results. The majority of the participants were female (70.2%; n=113). We found that younger care providers were less likely to report unmet needs (p = 0.011), and less likely to report burnout as measured by the MBI Emotional Exhaustion (p = 0.010) and Depersonalization (p = 0.009) subscales. Care providers with primary education were more likely to report burnout on the MBI Depersonalization subscale (p = 0.028). In addition, care providers who worked more often reported higher levels of met needs (p = 0.022), and burnout as measured on the MBI Depersonalization (p = 0.005) and Emotional Exhaustion (p = 0.018) subscales. Subjects residing in urban areas were more likely to report unmet needs (p = 0.007), and were also more likely to report burnout as measured on the MBI Emotional Exhaustion (p = 0.006) subscale.Conclusion. Older care providers who are unemployed and reside in cities and unemployed should be targeted with directed programmes to determine the category of unmet needs, and for personalized support. Care providers with these demographic characteristics should be targeted for occupational burnout prevention programmes.
Prevalence of pneumonia and its associated factors among under five children in East Africa. Meta-analysis and systemic review
Authors: Abate Biruk Beletew , Bimrew Melaku Getahun , Kasie Ayelign Mengesha , Wudu Mesfin Kassaw , Azmeraw Molla 
Published: February 2020

Abstract
Introduction Pneumonia is defined as an acute inflammation of the Lungs’ parenchymal structure. It is a major public health problem and the leading cause of morbidity and mortality in under-five children especially in developing countries. In 2015, it was estimated that about 102 million cases of pneumonia occurred in under-five children, of which 0.7 million were end up with death. Different primary studies in Eastern Africa showed the burden of pneumonia. However, inconsistency among those studies was seen and no review has been conducted to report the amalgamated magnitude and associated factors. Therefore, this review aimed to estimate the national prevalence and associated factors of neonatal sepsis in Eastern Africa. Objective The aim of this systematic review and meta-analysis was to assess the magnitude of pneumonia and its associated factors among under-five children in East Africa. Methods Using PRISMA guideline, we systematically reviewed and meta-analyzed studies that examined the prevalence and associated factors of pneumonia from PubMed, Cochrane library, and Google Scholar. Heterogeneity across the studies was evaluated using the Q and the I 2 test. A weighted inverse variance random-effects model was applied to estimate the national prevalence and the effect size of associated factors. The subgroup analysis was conducted by country, study design, and year of publication. A funnel plot and Egger’s regression test were used to see publication bias. Sensitivity analysis was also done to identify the impact of studies. Result A total of 34 studies with 87, 984 participants were used for analysis. The pooled prevalence of hypothermia in East Africa was 34% (95%CI; 23.80–44.21). Use of wood as fuel source (AOR= 1.53; 95%CI:1.30-1.77; I 2 = 0.0% ;P=0.465), cook food in living room (AOR= 1.47;95%CI:1.16-1.79; I 2 = 0.0% ;P=0.58), caring of a child on mother during cooking (AOR= 3.26; 95%CI:1.80-4.72; I 2 = 22.5% ;P=0.26), Being unvaccinated (AOR= 2.41; 95%CI:2.00-2.81; I 2 = 51.4% ;P=0.055), Child history of ARTI(AOR= 2.62; 95%CI:1.68-3.56; I 2 = 11.7% ;P=0.337) were identified factors of pneumonia. Conclusions The prevalence of pneumonia in Eastern Africa remains high. This review will help policy-makers and program officers to design pneumonia preventive interventions.
Metformin improves monosodium-iodoacetate-induced osteoarthritis via regulation of pain mediators and autophagy-lysosomal pathway.
Authors: Na Hyun Sik , Kwon Ji Ye , Lee Seung Hoon , Lee Seon-young , Jung KyungAh , Cho Keun Hyung , Choi Jeong-Won , Min Hong-Ki , Park Sung-Hwan , Kim Seok Jung , CHO MI-LA 
Published: February 2020

Abstract
Background Osteoarthritis (OA) is the most common degenerative arthritis associated with pain and cartilage destruction in the elderly. Its pathogenesis involves inflammatory pathways. Metformin is used to treat type 2 diabetes but also exhibits regulation of autophagy pathway. This study investigated the ability of metformin to ameliorate the severity of monosodium iodoacetate (MIA)-induced OA in rats. Methods Metformin was administered orally every day to rats with OA experimentally induced by intra-articular injection of MIA. Paw-withdrawal latency and threshold were used to assess pain severity. Cartilage damage and pain mediators in dorsal root ganglia were evaluated by histological analysis and a scoring system. Relative mRNA expression was measured by real-time PCR. Results Metformin ameliorated the progression of experimental OA and exhibited both anti-nociceptive properties and cartilage protection. The metformin-induced down-regulation of pain mediators, including CGRP, in dorsal root ganglia and protection against bone damage were also determined. In chondrocytes from OA patients, metformin decreased expression of genes encoding catabolic factors stimulated by interleukin-1β and inflammatory cell death factors and induced autophagosome–lysosome fusion phenotype. The combination therapy of metformin and celecoxib had significantly less cartilage damage than either the metformin alone group or the control group. Conclusions These results imply the possibility of therapeutic use of metformin in OA patients, based on its ability to suppress pain and protect cartilage.