bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robot-assisted laparoscopic ureteroneocystostomy combined to hysterectomy for intrinsic ureteral and deep pelvic endometriosis: Video presentation
Authors: Albanesi Gianluca , Baroni Clara , Cecchi Elena , Mogorovich Andrea , Salerno Maria Giovanna , Perutelli Alessandra 
Published: March 2020

Introduction: We present a case of posterior deep infiltrating endometriosis with intrinsic ureteral involvement in a 43-year-old patient treated by robot-assisted laparoscopic technique. To our knowledge, this is the second case of robot-assisted ureteroneocystostomy with concomitant hysterectomy reported in the literature1–5, yet the first one to be described with a video presentation. Case description: A 43-year-old woman with a past history of endometriosis treated laparoscopically was referred to our clinic for grade II–III left hydronephrosis. Pelvic ultrasound and magnetic resonance imaging demonstrated a 3-cm paracervical endometriotic nodule causing ureteral compression, another 1.6-cm endometriotic nodule at the rectosigmoid junction, an adenomyotic nodule in the anterior uterine wall, and an endometrioma on the right ovary. Given that the patient had an absolute contraindication to hormonal therapy, we opted for robot-assisted laparoscopic complete eradication of endometriosis. The procedure consisted in total hysterectomy with concomitant removal of the rectovaginal nodule, right adnexectomy, left salpingectomy, and left ureteral resection with ureteroneocystostomy. After 8 days, a cystography was accomplished to confirm anastomosis integrity, and the bladder catheter was removed. The patient was discharged on day 8 after a regular postoperative period. One year after the surgery, the patient refers wellbeing. Conclusion: The robotic approach in such a complex procedure as surgical treatment of ureteral and deep infiltrating endometriosis is feasible and safe with acceptable postoperative outcome. Further investigations with larger comparative cohorts that include cost analysis are needed to fully assess the benefits achieved by robotic assistance in patients with ureteral and deep infiltrating endometriosis.
Effect of Teaching Session on Resident Ability to Identify Anatomic Landmarks and Anterior Cruciate Ligament Footprint: A Study Using 3-Dimensional Modeling
Authors: Laverdiere Carl , Harvey Eric , Schupbach Justin , Boily Mathieu , Burman Mark , Martineau Paul A. 
Published: March 2020

Background: Femoral tunnel positioning in anterior cruciate ligament reconstruction (ACLR) is an intricate procedure that requires highly specific surgical skills. Purpose: To report the ability of residents to identify femoral landmarks and the native ACL footprint before and after a structured formal teaching session as a reflection of overall surgical skill training for orthopaedic surgery residents in Canada. Study Design: Controlled laboratory study. Methods: A total of 13 senior orthopaedic residents were asked to identify a femoral landmark and an ACL footprint on ten 3-dimensional (3D)–printed knee models before and after a teaching session during the fall of 2018. The 3D models were made based on actual patients with different anatomic morphologic features. ImageJ software was used to quantify the measurements, which were then analyzed through use of descriptive statistics. Results: Before and after the teaching session, residents attempted to identify a specific anatomic location (bifurcate and intercondylar ridge intersection) with a mean error per participant ranging from 5.00 to 10.95 mm and 4.79 to 12.13 mm in magnitude, respectively. Furthermore, before and after the teaching session, residents identified the specific position to perform the surgical procedure (ACL femoral footprint), with a mean error per participant ranging from 4.58 to 8.80 mm and 3.87 to 11.07 mm in magnitude, respectively. The teaching session resulted in no significant improvement in identification of either the intersection of the bifurcate and intercondylar ridges ( P = .9343 in the proximal-distal axis and P = .8133 in the anteroposterior axis) or the center of the femoral footprint ( P = .7761 in the proximal-distal axis and P = .9742 in the anteroposterior axis). Conclusion: Although a formal teaching session was combined with a hands-on session that entailed real surgical instrumentation and fresh cadaveric specimens, the intervention seemed to have no direct impact on senior residents’ performance or their ability to demonstrate the material taught. This puts into question the format and efficacy of present teaching methods. Also, it is possible that the 3D spatial perception required to perform these skills is not something that can be taught effectively through a teaching session or at all. Further investigation is required regarding the effectiveness and application of surgical skill laboratories and simulations on the competencies of orthopaedic residents.
Morphine alters respiratory control but not other key obstructive sleep apnoea phenotypes: a randomised trial
Authors: Martins Rodrigo T. , Carberry Jayne C. , Wang David , Rowsell Luke , Grunstein Ronald R. , Eckert Danny J. 
Published: March 2020

Accidental opioid-related deaths are increasing. These often occur during sleep. Opioids such as morphine may worsen obstructive sleep apnoea (OSA). Thus, people with OSA may be at greater risk of harm from morphine. Possible mechanisms include respiratory depression and reductions in drive to the pharyngeal muscles to increase upper airway collapsibility. However, the effects of morphine on the four key phenotypic causes of OSA (upper airway collapsibility (pharyngeal critical closure pressure;Pcrit), pharyngeal muscle responsiveness, respiratory arousal threshold and ventilatory control (loop gain) during sleep) are unknown.21 males with OSA (apnoea–hypopnoea index range 7–67 events·h−1) were studied on two nights (1-week washout) according to a double-blind, randomised, cross-over design (ACTRN12613000858796). Participants received 40 mg of MS-Contin on one visit and placebo on the other. Brief reductions in continuous positive airway pressure (CPAP) from the therapeutic level were delivered to induce airflow limitation during non-rapid eye movement (REM) sleep to quantify the four phenotypic traits. Carbon dioxide was deliveredvianasal mask on therapeutic CPAP to quantify hypercapnic ventilatory responses during non-REM sleep.Compared to placebo, 40 mg of morphine did not changePcrit(−0.1±2.4versus−0.4±2.2 cmH2O, p=0.58), genioglossus muscle responsiveness (−2.2 (−0.87 to −5.4)versus−1.2 (−0.3 to −3.5) μV·cmH2O−1, p=0.22) or arousal threshold (−16.7±6.8versus−15.4±6.0 cmH2O, p=0.41), but did reduce loop gain (−10.1±2.6versus−4.4±2.1, p=0.04) and hypercapnic ventilatory responses (7.3±1.2versus6.1±1.5 L·min−1, p=0.006).Concordant with recent clinical findings, 40 mg of MS-Contin does not systematically impair airway collapsibility, pharyngeal muscle responsiveness or the arousal threshold in moderately severe OSA patients. However, consistent with blunted chemosensitivity, ventilatory control is altered.
Self-healing coatings for inhibiting corrosion of ferrous metals exposed to preservative-treated bamboo
Authors: Wang Qingping , Cao Jinzhen , Liu Xing’e , Yang Shumin , Jiang Mingliang 
Published: March 2020

AbstractThis study aims to provide an effective method of inhibiting the corrosion of ferrous metals exposed to preservative-treated bamboo, thereby prolonging material service life and reducing maintenance costs. The synthesis and characterization of microcapsules were first described. The characterization included the particle size, surface morphology, thermal stability, and core content of microcapsules. The results showed that microcapsules had good thermal stability and high core loading. Then, the self-healing performance and corrosion resistance of self-healing coatings were evaluated. The results indicated that self-healing coatings can identify and heal the damage automatically and had a more positive effect on inhibiting metal corrosion. Also, the mechanical properties of self-healing coatings were analyzed. The results demonstrated that microcapsule embedment did not almost affect the mechanical properties of self-healing coatings.
Determination of the physical and mechanical properties of moso, guadua and oldhamii bamboo assisted by robotic fabrication
Authors: Lorenzo Rodolfo , Godina Martha , Mimendi Leonel , Li Haitao 
Published: March 2020

AbstractThe large-scale urbanisation taking place in the developing world requires the construction industry to adopt alternative non-conventional renewable materials to reduce the unsustainable level of greenhouse gas emissions associated with the production of industrialised building materials. Bamboo is one of the most promising non-conventional building materials endemic to most developing countries, but there is still insufficient consistent information on the physical and mechanical properties of the numerous species suitable for construction. This study shows the potential of robotic fabrication to accelerate testing programmes on small clear samples of bamboo required to compare physical and mechanical properties across different species and differing plantation management practices. This fabrication method is applied on an experimental testing programme to determine the characteristic values of density, compressive strength, elastic modulus and shear strength ofPhyllostachys pubescens(moso),Guadua angustifoliaKunth (guadua) andGuadua angustifolia(oldhamii). The efficient development of comprehensive experimental datasets of clear samples of bamboo is fundamental to inform the development of future design guidelines for bamboo as a construction material.
Enzyme-Free Glucose Biosensors Based on MoS2 Nanocomposites
Authors: He Weijie , Huang Yixuan , Wu Jiang 
Published: March 2020

AbstractHigh-performance glucose biosensors are highly desired for healthcare. To meet these demands, glucose biosensors, particularly enzyme-free glucose biosensors, have received much attention. Two-dimensional materials, e.g., graphene, with high surface area, excellent electrical properties, and good biocompatibility, have been the main focus of biosensor research in the last decade. This review presents the recent progress made in enzyme-free glucose biosensors based on MoS2 nanocomposites. Two different techniques for glucose detections are introduced, with an emphasis on electrochemical glucose biosensors. Challenges and future perspectives of MoS2 nanocomposite glucose biosensors are also discussed.
Juvenile diet quality and intensity of sexual conflict in the mite Sancassania berlesei
Authors: Łukasiewicz Aleksandra 
Published: March 2020

Abstract
Background
Differing evolutionary interests of males and females may result in sexual conflict, whereby traits or behaviours that are beneficial for male reproductive success (e.g., traits related to male-male competition) are costly for females. Since sexual conflict may play an important role in areas such as speciation, population persistence or evolution of life history traits, understanding what factors modulate the intensity of sexual conflict is important. This study aims to examine juvenile diet quality as one of the underestimated ecological factors that may affect the intensity of sexual conflict via individual conditions. I used food manipulation during the development of the mite Sancassania berlesei to investigate the effects on male reproductive behaviour and competitiveness, male-induced harm to female fitness and female resistance to this harm.

Results
Males that were exposed to low-quality food started mating later than the control males, and number of their mating attempts were lower compared to those of control males. Moreover, males from the low-quality diet treatment sired fewer offspring under competition than males from the control treatment. However, the fitness of females exposed to males reared on a poor diet did not differ from that of females mated with control males. Furthermore, female diet quality did not alter their resistance to male-induced harm.

Conclusion
Overall, diet quality manipulation affected male reproductive behaviour and mating success. However, I found no evidence that the intensity of sexual conflict in S. berlesei depends on male or female conditions. Investigating a broader range of environmental factors will provide a better understanding of sexual conflict dynamics and its feedback into associated evolutionary mechanisms.
Hepatic transcriptome of the freeze-tolerant Cope’s gray treefrog, Dryophytes chrysoscelis: responses to cold acclimation and freezing
Authors: do Amaral M. Clara F. , Frisbie James , Crum Raphael J. , Goldstein David L. , Krane Carissa M. 
Published: March 2020

AbstractBackgroundCope’s gray treefrog,Dryophytes chrysoscelis, withstands the physiological challenges of corporeal freezing, partly by accumulating cryoprotective compounds of hepatic origin, including glycerol, urea, and glucose. We hypothesized that expression of genes related to cryoprotectant mobilization and stress tolerance would be differentially regulated in response to cold. Using high-throughput RNA sequencing (RNA-Seq), a hepatic transcriptome was generated forD. chrysoscelis, and gene expression was compared among frogs that were warm-acclimated, cold-acclimated, and frozen.ResultsA total of 159,556 transcripts were generated; 39% showed homology with known transcripts, and 34% of all transcripts were annotated. Gene-level analyses identified 34,936 genes, 85% of which were annotated. Cold acclimation induced differential expression both of genes and non-coding transcripts; freezing induced few additional changes. Transcript-level analysis followed by gene-level aggregation revealed 3582 differentially expressed genes, whereas analysis at the gene level revealed 1324 differentially regulated genes. Approximately 3.6% of differentially expressed sequences were non-coding and of no identifiable homology. Expression of several genes associated with cryoprotectant accumulation was altered during cold acclimation. Of note, glycerol kinase expression decreased with cold exposure, possibly promoting accumulation of glycerol, whereas glucose export was transcriptionally promoted by upregulation of glucose-6-phosphatase and downregulation of genes of various glycolytic enzymes. Several genes related to heat shock protein response, DNA repair, and the ubiquitin proteasome pathway were upregulated in cold and frozen frogs, whereas genes involved in responses to oxidative stress and anoxia, both potential sources of cellular damage during freezing, were downregulated or unchanged.ConclusionOur study is the first to report transcriptomic responses to low temperature exposure in a freeze-tolerant vertebrate. The hepatic transcriptome ofDryophytes chrysoscelisis responsive to cold and freezing. Transcriptomic regulation of genes related to particular pathways, such as glycerol biosynthesis, were not all regulated in parallel. The physiological demands associated with cold and freezing, as well as the transcriptomic responses observed in this study, are shared with several organisms that face similar ecophysiological challenges, suggesting common regulatory mechanisms. The role of transcriptional regulation relative to other cellular processes, and of non-coding transcripts as elements of those responses, deserve further study.
The relationship between symptom burden and systemic inflammation differs between male and female athletes following concussion
Authors: Di Battista Alex P. , Churchill Nathan , Rhind Shawn G. , Richards Doug , Hutchison Michael G. 
Published: March 2020

AbstractBackgroundInflammation appears to be an important component of concussion pathophysiology. However, its relationship to symptom burden is unclear. Therefore, the purpose of this study was to evaluate the relationship between symptoms and inflammatory biomarkers measured in the blood of male and female athletes following a sport-related concussion (SRC).ResultsForty athletes (n = 20 male, n = 20 female) from nine interuniversity sport teams at a single institution provided blood samples within one week of an SRC. Twenty inflammatory biomarkers were quantitated by immunoassay. The Sport Concussion Assessment Tool version 5 (SCAT-5) was used to evaluate symptoms. Partial least squares (PLS) analyses were used to evaluate the relationship(s) between biomarkers and symptoms. In males, a positive correlation between interferon (IFN)-γ and symptom severity was observed following SRC. The relationship between IFN-γ and symptoms was significant among all symptom clusters, with cognitive symptoms displaying the largest effect. In females, a significant negative relationship was observed between symptom severity and cytokines IFN-γ, tumor necrosis factor (TNF)-α, and myeloperoxidase (MPO); a positive relationship was observed between symptom severity and MCP-4. Inflammatory mediators were significantly associated with all symptom clusters in females; the somatic symptom cluster displayed the largest effect.ConclusionThese results provide supportive evidence of a divergent relationship between inflammation and symptom burden in male and female athletes following SRC. Future investigations should be cognizant of the potentially sex-specific pathophysiology underlying symptom presentation.
The low-copy nuclear gene Agt1 as a novel DNA barcoding marker for Bromeliaceae
Authors: Bratzel Fabian , Heller Sascha , Cyrannek Nadine , Paule Juraj , Leme Elton M. C. , Loreth Anna , Nowotny Annika , Kiefer Markus , Till Walter , Barfuss Michael H. J. , Lexer Christian , Koch Marcus A. , Zizka Georg 
Published: March 2020

Abstract
Background
The angiosperm family Bromeliaceae comprises over 3.500 species characterized by exceptionally high morphological and ecological diversity, but a very low genetic variation. In many genera, plants are vegetatively very similar which makes determination of non flowering bromeliads difficult. This is particularly problematic with living collections where plants are often cultivated over decades without flowering. DNA barcoding is therefore a very promising approach to provide reliable and convenient assistance in species determination. However, the observed low genetic variation of canonical barcoding markers in bromeliads causes problems.

Result
In this study the low-copy nuclear gene Agt1 is identified as a novel DNA barcoding marker suitable for molecular identification of closely related bromeliad species. Combining a comparatively slowly evolving exon sequence with an adjacent, genetically highly variable intron, correctly matching MegaBLAST based species identification rate was found to be approximately double the highest rate yet reported for bromeliads using other barcode markers.

Conclusion
In the present work, we characterize Agt1 as a novel plant DNA barcoding marker to be used for barcoding of bromeliads, a plant group with low genetic variation. Moreover, we provide a comprehensive marker sequence dataset for further use in the bromeliad research community.
Safety and outcome of gastrostomy tube placement in patients with Loeys-Dietz syndrome
Authors: Frischmeyer-Guerrerio Pamela A. , MacCarrick Gretchen , Dietz Harry C. , Stewart F. Dylan , Guerrerio Anthony L. 
Published: March 2020

Abstract
Background
Loeys-Dietz syndrome (LDS) is a systemic connective tissue disease (CTD) associated with a predisposition for intestinal inflammation, food allergy, and failure to thrive, often necessitating nutritional supplementation via gastrostomy tube. Poor wound healing has also been observed in in some patients with CTD, potentially increasing the risk of surgical interventions. We undertook to determine the safety and efficacy of gastrostomy tube placement in this population.

Methods
We performed a retrospective cohort study of 10 LDS patients who had a total of 12 gastrostomy tubes placed.

Results
No procedural complications occurred, although one patient developed buried bumper syndrome in the near post-procedural time period and one patient had a small abscess at a surgical stitch. Most patients exhibited improvements in growth, with a median immediate improvement in BMI Z-score of 0.2 per month following the institution of gastrostomy tube feedings. Those with uncontrolled inflammation due to inflammatory bowel disease or eosinophilic gastrointestinal disease showed the least benefit and in some cases failed to demonstrate significant weight gain despite nutritional supplementation.

Conclusions
Gastrostomy tube placement (surgical or endoscopic) is a generally safe and a reasonable therapeutic option for patients with LDS despite their underlying CTD.
Informal caregivers’ views on the quality of healthcare services provided to older patients aged 80 or more in the hospital and 30 days after discharge
Authors: Lilleheie Ingvild , Debesay Jonas , Bye Asta , Bergland Astrid 
Published: March 2020

AbstractBackgroundIn the European Union (EU), informal caregivers provide 60% of all care. Informal caregiving ranges from assistance with daily activities and provision of direct care to helping care recipients to navigate within complex healthcare and social services systems. While recent caregiver surveys document the impact of informal caregivers, systematic reviews show that they have unmet needs.Because of the political desire to reduce the length of hospital stays, older patients are discharged from the hospital ‘quicker and sicker’ than before. The transition between different levels of the healthcare system and the period after hospital discharge is critical for elderly patients.Caregivers’ perspectives on the quality of older patients’ care journeys between levels of the healthcare system may provide valuable information for healthcare providers and policymakers. This study aims to explore older patient’s informal caregivers’ views on healthcare quality in the hospital and in the first 30 days after hospitalisation.MethodWe conducted semi-structured individual interviews with 12 participants to explore and describe informal caregivers’ subjective experiences of providing care to older relatives. The interviews were then transcribed and analysed thematically.ResultsThe analysis yielded the overarching theme ‘Informal caregivers – a health service alliance – quality contributor’, which was divided into four main themes: ‘Fast in, fast out’, ‘Scant information’, ‘Disclaimer of responsibility’ and ‘A struggle to secure professional care’. The healthcare system seemed to pay little attention to ensuring mutual understandings between those involved in discharge, treatment and coordination. The participants experienced that the healthcare providers’ main focus was on the patients’ diseases, although the health services are supposed to view patients holistically.ConclusionBased on the information given by informal caregivers, health services must take into account each person’s needs and preferences. To deliver quality healthcare, better coordination between inter-professional care teams and the persons they serve is necessary. Health professionals must strengthen the involvement of caregivers in transitions between care and healthcare. Future work should evaluate targeted strategies for formal caregivers to cooperate, support and empower family members as informal caregivers.