bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skin flora and bacterial contamination of diversion pouch and recovered platelet components in Moroccan blood donors
Authors: EL Mehdaoui Fatna , Benajiba Mohammed , Boulahdid Safia , El Hattimy Faiçal , Soulaymani Abdelmajid , Alami Raouf 
Published: July 2020

AbstractObjectiveThe objective of this study was to evaluate bacterial reduction procedures used during whole‐blood donations in Morocco.BackgroundBacterial contamination still poses serious challenges to blood safety, especially in countries with limited resources.MethodsIn the first part of this study, we analysed 233 swab samples taken from blood donors' antecubital fossa. After donation, a second batch of samples was analysed from the diversion pouches of corresponding donors. In addition, we searched for the prevalence of bacterial contamination in 568 randomly chosen platelet components at their expiration date in order to control for the entire blood unit preparation process.ResultsThe most frequently found bacterial species at the antecubital fossa of healthy blood donors were coagulase‐negative Staphylococcus, aerophilic Corynebacterium, Staphylococcus aureus, Bacillus sp. and Micrococcus sp. After donation, 5.15% of the diversion pouches were contaminated with bacterial species, the most notable being Bacillus sp., aerophilic Corynebacterium, coagulase‐negative Staphylococcus, Staphylococcus aureus and Pseudomonas aeruginosa. Of 568 platelet components, 18 were contaminated with three bacterial species: Bacillus sp., coagulase‐negative Staphylococcus and Staphylococcus aureus.ConclusionAll three bacterial species found in platelet components were detected on the skin of blood donors. Serious measures need to be taken and enforced to ensure blood safety.
Model‐assisted identification of metabolic engineering strategies for Jatropha curcas lipid pathways
Authors: Correa Sandra M. , Alseekh Saleh , Atehortúa Lucía , Brotman Yariv , Ríos‐Estepa Rigoberto , Fernie Alisdair R. , Nikoloski Zoran 
Published: July 2020

SUMMARYEfficient approaches to increase plant lipid production are necessary to meet current industrial demands for this important resource. While Jatropha curcas cell culture can be used for in vitro lipid production, scaling up the system for industrial applications requires an understanding of how growth conditions affect lipid metabolism and yield. Here we present a bottom‐up metabolic reconstruction of J. curcas supported with labeling experiments and biomass characterization under three growth conditions. We show that the metabolic model can accurately predict growth and distribution of fluxes in cell cultures and use these findings to pinpoint energy expenditures that affect lipid biosynthesis and metabolism. In addition, by using constraint‐based modeling approaches we identify network reactions whose joint manipulation optimizes lipid production. The proposed model and computational analyses provide a stepping stone for future rational optimization of other agronomically relevant traits in J. curcas.
Risk of invasive candidiasis with prolonged duration of ICU stay: a systematic review and meta-analysis
Authors: Zhang Zhidan , Zhu Ran , Luan Zhenggang , Ma Xiaochun 
Published: July 2020

ObjectiveThis study aimed to evaluate the duration of intensive care unit (ICU) stay prior to onset of invasive candidiasis (IC)/candidaemia.DesignSystematic review and meta-analysis.Data sourcesPubMed, Cochrane, Embase and Web of Science databases were searched through June 2019 to identify relevant studies.Eligibility criteriaAdult patients who had been admitted to the ICU and developed an IC infection.Data extraction and synthesisThe following data were extracted from each article: length of hospital stay, length of ICU stay, duration of ICU admission prior to candidaemia onset, percentage of patients who received antibiotics and duration of their antibiotic therapy prior to candidaemia onset, and overall mortality. In addition to the traditional meta-analyses, meta-regression was performed to explore possible mediators which might have contributed to the heterogeneity.ResultsThe mean age of patients ranged from 28 to 76 years across selected studies. The pooled mean duration of ICU admission before onset of candidaemia was 12.9 days (95% CI 11.7 to 14.2). The pooled mean duration of hospital stay was 36.3±5.3 days (95% CI 25.8 to 46.7), and the pooled mean mortality rate was 49.3%±2.2% (95% CI 45.0% to 53.5%). There was no significant difference in duration of hospital stay (p=0.528) or overall mortality (p=0.111), but a significant difference was observed in the mean length of ICU stay (2.8 days, p<0.001), between patients with and withoutCandida albicans. Meta-regression analysis found that South American patients had longer duration of ICU admission prior to candidaemia onset than patients elsewhere, while those in Asia had the shortest duration.ConclusionsPatients with IC are associated with longer ICU stay, with the shortest duration of ICU admission prior to the candidaemia onset in Asia. This shows a more proactive strategy in the diagnosis of IC should be considered in caring for ICU patients.
Meniscal tear outcome Study (METRO Study): a study protocol for a multicentre prospective cohort study exploring the factors which affect outcomes in patients with a meniscal tear
Authors: Ahmed Imran , Bowes Mike , Hutchinson Charles E , Parsons Nicholas , Staniszewska Sophie , Price Andrew James , Metcalfe Andrew 
Published: July 2020

IntroductionThis study is designed to explore the baseline characteristics of patients under 55 years of age with a meniscal tear, and to describe the relationship between the baseline characteristics and patient-reported outcome measures (PROMs) over 12 months. Research has highlighted the need for a trial to investigate the effectiveness of arthroscopic meniscectomy in younger patients. Before this trial, we need to understand the patient population in greater detail.Methods and analysisThis is a multicentre prospective cohort study. Participants aged between 18 and 55 years with an MRI confirmed meniscal tear are eligible for inclusion. Baseline characteristics including age, body mass index, gender, PROMs duration of symptoms and MRI will be collected. The primary outcome measure is the Western Ontario Meniscal Evaluation Tool at 12 months. Secondary outcome measures will include PROMs such as EQ5D, Knee Injury and Osteoarthritis Outcome Score and patient global impression of change score at 3, 6 and 12 months.Ethics and disseminationThe study obtained approval from the National Research Ethics Committee West Midlands—Black Country research ethics committee (19/WM/0079) on 12 April 2019. The study is sponsored by the University of Warwick. The results will be disseminated via peer-reviewed publication.Trial registration numberUHCW R&D Reference: IA428119. University of Warwick Sponsor ID: SC.08/18–19
Australian treatment outcome study: protocol for the 18–20-year follow-up of a prospective longitudinal cohort examining the natural history of heroin dependence and associated mortality, psychiatric and physical health, and health service use
Authors: Marel Christina , Mills Katherine , Visontay Rachel , Wilson Jack , Darke Shane , Ross Joanne , Slade Tim , Haber Paul S , Haasnoot Katherine , Keaveny Madeleine , Tremonti Chris , Teesson Maree 
Published: July 2020

IntroductionOpioid dependence is a global health priority, currently making the biggest contribution to drug-related deaths. The chronic, long-term persistence of heroin dependence over the life course requires investigation in prospective longitudinal studies, to better understand patterns and predictors of remission and relapse, as well as the impact of changes in substance use on a range of physical and mental health outcomes. Such knowledge is critical in order to identify modifiable risk factors that can be targeted for intervention. Crucial unanswered questions include the following: What are the long-term rates of mortality? What are the long-term patterns and predictors of heroin use, remission, psychiatric health and health service use? What are the long-term physical health consequences of heroin use?Methods and analysisThe 18–20-year follow-up of the Australian Treatment Outcome Study (ATOS) cohort will examine the natural history of heroin dependence of an existing cohort of 615 people with heroin dependence, who were recruited into the study in 2001–2002. Five waves of follow-up interviews have since been completed, at 3-month, 1-year, 2-year, 3-year and 10–11-year post-baseline. At the 18–20-year follow-up, the ATOS cohort is (on average) approaching their 50s and an average of 30 years have passed since they first used heroin. The 18–20-year follow-up will consist of: (1) a structured interview; (2) physical health assessment; and (3) data linkage. The results of this follow-up will improve our understanding and management of age-related disorders in this population, which if not addressed in the immediate future, has the capacity to overwhelm treatment centres and aged care facilities.Ethics and disseminationEthical approval has been granted for the study (Sydney Local Health District Royal Prince Alfred Zone, Human Research Ethics Committee X18-0512 & HREC/18/RPAH/733). The results of the study will be disseminated through published manuscripts, bulletins and technical reports, as well as conference, seminars, webinar and workshop presentations.
Changing speed of reduction in under-5 mortality rates over the 20th century
Authors: Karlsson Omar , Dribe Martin , Subramanian S V 
Published: July 2020

BackgroundDeclines from high levels of under-5 mortality rate (U5MR) first occurred in Western Europe. Knowledge and technologies gained from early mortality reductions could accelerate the U5MR decline for countries that followed. We explored whether average annual reduction (AAR) in U5MR increased between countries over time in the 20th century.MethodsWe used U5MR time series from the Human Mortality Database and United Nations for 110 countries experiencing a decline from 100 to 50 under-5 deaths per 1000 live births during the 20th century.ResultsBetween 1907 and 1938, the AAR was 2.61 (95% CI 2.09, 3.13) deaths per 1000 live births per year on average and increased by 0.06 (95% CI 0.02, 0.10) deaths for each year that passed before the decline started. Countries going through the decline in 1938–1968 and 1968–1999 showed an AAR of 3.96 and 3.67 (95% CI 3.37, 4.54 and 3.26, 4.07), respectively, with no increase in AAR.ConclusionsAcceleration in U5MR reduction was apparent in today’s high-income countries, indicating that greater similarities and capacity may have facilitated the adaptation of mortality reducing knowledge and technologies. Greater emphasis on simple and individual-level interventions or more difficult circumstances may also explain the lack of acceleration in mortality reduction after 1950.
Human papilloma viruses infection among adolescent females perinatally infected with HIV in Côte d’Ivoire
Authors: Tchounga Boris , Horo Apollinaire , Boni Simon , Tanon Aristophane , Folquet Madeleine Amorissani , Garrigue Isabelle , Leroy Valeriane , Dabis François , Ekouevi Didier , Jaquet Antoine 
Published: July 2020

Background
Cervical cancer prevention strategies recommend human papilloma virus (HPV) vaccination for female adolescents prior to their sexual debut. While HIV is a major risk factor for HPV infection in women of childbearing age, its prevalence among HIV-infected adolescent female is mostly unknown. This study aimed to describe the HPV prevalence and correlates among perinatally HIV-infected adolescent females prior to HPV immunisation.

Methods
A cross-sectional survey was conducted from January to June 2016, in the four major paediatric HIV clinics of Abidjan, Côte d’Ivoire. All HIV-infected females aged 11–16 years were approached to participate in the study. A questionnaire assessing sexual behaviours and genital hygiene practices was administered to participants completed with a systematic vaginal swab collection. HPV genotyping was performed using the Anyplex II HPV28 Detection (Seegene). A logistic regression analysis was performed to identify factors associated with the presence of HPV infection. HPV immunisation was proposed free of charge to all participants.

Results

A total of 250 participants were included, with a median age of 13 years (IQR 11–14). Among them, 237 (94.8%) were on antiretroviral treatment with a median CD4 count of 660 (IQR 439–914) cells/mm
3
. The overall prevalence of at least one HPV was 3.6% (95% CI 1.6 to 6.7) and the prevalence of at least one carcinogenic HPV was 2.8% (95% CI 0.7 to 4.8). Vaginal cleansing was reported by 75 (30%) of participants, with a median age at initiation of 12 years (IQR 10–13). Sexual activity was self-reported by 12 (4.8%) participants with a median age at sexual debut of 11 years (IQR 10–14). HPV infection was associated with vaginal cleansing (adjusted OR=7.0 (95% CI 1.4 to 31.6)).

Conclusion
The reported low prevalence of carcinogenic HPV infections supports the appropriateness of HPV immunisation in this population. The reported association between cleansing practices and HPV infection deserves further prospective longitudinal studies.
Clinical features and long‐term follow‐up of 70 cases of canine idiopathic eosinophilic lung disease
Authors: Casamian‐Sorrosal Domingo , Silvestrini Paolo , Blake Rachel , Kortum Andre , Watson Penny J , Martínez Yolanda , Lopez Alvarez Jordi , Keegan Sarah 
Published: July 2020

BackgroundCanine idiopathic eosinophilic lung disease (ELD) is sparsely documented in the literature.MethodsClinical presentation and outcome of dogs diagnosed with ELD (eosinophilic bronchitis or eosinophilic bronchopneumonia) were reviewed. Subgroups were made based on chronicity of clinical signs and findings of thoracic imaging: NCI (no changes in thoracic imaging), BRON (bronchial/peribronchial pattern), INT (bronchointerstitial/interstitial/alveolar).ResultsSeventy cases were included. There were more young to adult, crossbreed and female dogs. Compared with the other two groups NCI dogs showed lower bronchoalveolar lavage fluid eosinophilic pleocytosis and absence of circulating eosinophilia, bronchiectasis or death due to respiratory disease. All dogs responded clinically to corticosteroids. Median treatment duration was four months. Remission (no clinical signs after treatment discontinuation for >one month) and long‐term remission (>six months) was achieved in 60 per cent, and 51 per cent of patients, respectively. Relapse occurred in 26 per cent of cases after remission but was rare (3 per cent) after long‐term remission. The one‐year, two‐year and four‐year survival to death due to respiratory disease was 98 per cent, 97 per cent and 91 per cent, respectively.ConclusionPrognosis and initial clinical response for ELD was generally good although achievement of long‐term remission was only seen in 51 per cent of dogs. Different outcomes based on chronicity of signs, corticosteroid dose, thoracic imaging abnormalities and other clinical variables were not appreciated.
Kidney structure and function in dilating vesicoureteral reflux patients with anorectal malformation
Authors: Miyake Yuichiro , Koga Hiroyuki , Lane Geoffrey J , Yamataka Atsuyuki 
Published: July 2020

BackgroundWe assessed the impact of anorectal malformation (ARM) on the kidneys of children with dilating vesicoureteral reflux (D-VUR) ≥grade III using a simple dimercaptosuccinic acid (DMSA) scintigraphy scan based renal dysfunction score (RDS).MethodsThe medical records of 121 patients with D-VUR treated between 2000 and 2014 were reviewed retrospectively. After excluding patients with secondary D-VUR (n=18), presence of ARM was used to create two groups: ARM+ (n=12 cases; 15 ureters) and ARM– (n=91 cases; 131 ureters). Types of ARM, grades of D-VUR, bladder and bowel dysfunction (BBD) and RDS were compared.ResultsPatient demographics, mean follow-up, grades of D-VUR and history of urinary tract infections were not significantly different. BBD was significantly higher in ARM+ (41.7% versus 7.7%, p=0.0006). RDS was significantly higher in ARM+ (p=0.036). Grades of D-VUR were significantly lower in ARM– with low RDS (p=0.008). During follow-up, changes in DMSA uptake over time were not observed in ARM+.ConclusionsWhile renal cortical lesions were correlated with grade of D-VUR in ARM– and RDS was significantly higher in ARM+, BBD did not appear to contribute to progressive renal dysfunction as is commonly believed. In fact, no progression in renal cortical lesions was observed in ARM+ based on RDS data. Renal cortical lesions may possibly be a feature of ARM, a topic that warrants further study.
Social-ecological changes and implications for understanding the declining beluga whale (Delphinapterus leucas) harvest in Aklavik, Northwest Territories
Authors: Worden Elizabeth , Pearce Tristan , Gruben Michelle , Ross Dorothy , Kowana Clarence , Loseto Lisa 
Published: July 2020

Subsistence is the basis for food access for Inuvialuit in the western Canadian Arctic and has strong economic, dietary, and cultural importance. Inuvialuit harvest beluga whale (Delphinapterus leucas (Pallas, 1776)) from the eastern Beaufort beluga population during summer months within parameters established through co-management. Over the past thirty years there has been a dramatic decline in the number of beluga harvested by Inuvialuit from the community of Aklavik, Northwest Territories. This paper investigates the potential drivers of change, both social and ecological, affecting the beluga harvest. Data were collected using 32 semi-directed interviews and experiential learning. Results revealed that ecological changes, notably coastal erosion at preferred whaling camps and unpredictable and severe weather have made harvesting more difficult, expensive, and often impractical. These changes are being experienced together with social changes including the loss of elders and their knowledge, and changing values and motivations for harvesting beluga. We conclude that no one driver is responsible for the decline in the beluga harvest, but rather it is the result of multiple social-ecological changes operating across scales that affect the feasibility of the harvest and motivation to participate. Isumatuyut ikayuqtuat avvakuyaa niqimun pimagaa Inuvialuit uataani Canadian Arcticmi nakuuyuq manik, niqilu, inuusiq nakuruallaktuaq. Inuvialuit katitait qilalugaq (Delphinapterus leucas (Pallas, 1776)) kivanmun Beaufort qilalugaq suli auyaqmi savaktiit. Sivulliqmi inuinnaq-qulit ukiuqmi mikliyuat tapqua qilalugaq katitait Inuvialuit Aklavik, Northwest Territoriesmi. Una makpiraaq ilisaqtuat anguniaqtuat, iluqatik inuuniarvikmi imaqmilu, tutqaanaittuq qilalugaq katitait. Kisitchiun katitait atugaa inuinnaq-qulit-malruknik apiqsiyuat asulu ilisaqtuat. Taimaagaa takupkagaa imaqmilu allauyuaq, taamna sallirq maqaigaa nuna taamna qilalugaqmun tanmaaq asulu allauyuaq silakluk asiin katitait tutqaanaittuq, akituyuqlu tutqaanaittuq. Taamna allauyuat illisaktuat atautchikun inuuniarvik ila tuquyuat innait asulu ilisimaruat, allauyuat pitqusiqlu ikayuqtuaq katitait qilalugaq. Uvagut taimagaa anguniaqti mikliyuat qilalugaq katitait taimagaa inugiaktut inuuniarvikmi imaqmilu allauyuaq savaktuat tutqaanaittuq asulu katitait ikayuqtuat ila taputiyaa.
COMPUTATIONAL MODELING AND THEORETICAL ANALYSIS OF NONLINEAR FRACTIONAL ORDER PREY–PREDATOR SYSTEM
Authors: ALI AMJAD , SHAH KAMAL , ALRABAIAH HUSSAM , SHAH ZAHIR , RAHMAN GHAUS UR , ISLAM SAEED 
Published: July 2020

In this work, we investigate a nonlinear fractional order Lotka–Voltera prey–predator model. It is also worth mentioning that we develop some results about the existence of the solution through fixed point theory. After investigating the existence results, we also develop a suitable technique with the help of Laplace transform coupled with Adomain decomposition in order to obtain the approximate solutions for the underlying model. Here, it should be kept in mind that we study the proposed model subject to variable coefficients. The results obtained via the aforesaid techniques are illustrated graphically via Matlab-16 corresponding to different fractional order by taking two cases. Reviewers list: Prof. Dumitru Baleanu (Cancaya University Ankara Turkey), Email: dumitru@cankaya.edu.tr , Dr. Anwar Zeb (COMSAT, University Pakistan), Email: anwar@cuiatd.edu.pk , Prof. J. F. Gómez-Aguilar (CONACyT-Tecnológico Nacional de México/CENIDET. Interior Internado Palmira S/N, Col. Palmira, C. P. 62490, Cuernavaca, Morelos, México), Email: jgomez@cenidet.edu.mx .
Development of synthetic protocols for porphyrins and their analogs based on distorted structures — a SPP/JPP Young Investigator Award paper
Authors: Hiroto Satoru 
Published: July 2020

Creation of novel [Formula: see text]-conjugated molecules is an important research topic. I describe in this account an approach to this aim that is based on the use of the distorted conformation of porphyrins. Planarization of distorted molecules enables the synthesis of heteroatom-containing porphyrin derivatives. Furthermore, dearomatization reaction proves effective to construct distorted conformations from planar [Formula: see text]-conjugated molecules under mild reaction conditions. According to this protocol, we have succeeded in the synthesis of heteroatom-containing curved-[Formula: see text] conjugated molecules that had never been achieved by conventional protocols. In particular, a nitrogen-embedded buckybowl is the first example of a buckybowl having a heteroatom in its central position, which exhibits unique properties due to the incorporation of the heteroatom in its curved [Formula: see text]-surface.
ECONOMIC IMPACTS OF CLIMATE CHANGE AND AIR POLLUTION IN CHINA THROUGH HEALTH AND LABOR SUPPLY PERSPECTIVE: AN INTEGRATED ASSESSMENT MODEL ANALYSIS
Authors: WANG CAN , HUANG HAI , CAI WENJIA , ZHAO MENGZHEN , LI JIN , ZHANG SHIHUI , LIU YUAN 
Published: July 2020

An energy supply dominated by the use of fossil fuels causes both climate change and air pollution, which have negative impacts on human capital via both health and productivity. In addition, different people are affected differently because of factors such as age, gender and education level. To enhance the understanding of the benefits of low carbon transition from the labor supply perspective and help to identify strategies of collaborative control for CO2 and local air pollutants in China, an integrated assessment model linking the air quality module and the health impact module with a disaggregated labor sector computable general equilibrium (CGE) economic system is developed and applied in this study. Results show some key findings. First, renewable energy development and carbon capture and storage (CCS) technologies will contribute significantly to GDP in terms of their impact on air quality improvement by 0.99% and 0.54%, respectively, in 2050. Second, due to differences in labor composition, air pollution has, and will continue to have, the greatest impact on sectors with a higher proportion of male and lower-educated workers — such as the coal sector, and it will have the least impact on sectors with a higher proportion of female and higher-educated workers — such as the public administration sector. Third, the different impacts of sector output will increase economic inequality. Highlights • The economic impact of climate change and air pollution is assessed. • A CGE model with disaggregated labor sectors is developed. • The secondary industry is most affected by pollution from a health perspective. • Low-income groups suffer the largest loss of income due to pollution. • A low carbon policy will improve air quality and economic equity.