bluuebunny/crossref_metadata_2025_split huggingface dataset:
Snowdrift Landscape Patterns: An Arctic Investigation
Authors: Parr Charles , Sturm Matthew , Larsen Chris 
Published: October 2020

AbstractBetween 2012 and 2018 we mapped near‐peak seasonal snow depths across two swaths covering 126 km2 in Northern Alaska using aerial structure‐from‐motion photogrammetry and lidar surveys. The surveys were validated by over a hundred thousand ground‐based depth measurements. Using a quantitative method for identifying drift areas, we conducted a snowdrift census that showed on average 18% of the study area is covered by snowdrifts each winter, with 40% of the snow‐water‐equivalent contained in the drifts. Within the census we identified six types of drifts, some of which fill each winter, others which do not. The seasonal drift evolution was distinctly different in the two swaths, a result largely explained by topographic differences. Using four metrics from the field of image quality analysis, we tested the year‐to‐year fidelity of these drift patterns, finding overall high year‐to‐year similarity, but with higher similarity values for filling drifts, and higher similarity values in one swath versus the other, again a function of the topography. These high drift fidelity values are best explained by climatically convergent winter‐cumulative windblown snow fluxes interacting with drift traps to produce the same drifts year after year. However, due to the existence of filling versus nonfilling drifts, and a predicted increasing frequency of rain‐on‐snow events in the Arctic, future snowdrift patterns and drift evolution pathways in the Arctic could diverge from those of today, with direct hydrologic impacts.
Predicting Variable Contributing Areas, Hydrological Connectivity, and Solute Transport Pathways for a Canadian Prairie Basin
Authors: Costa Diogo , Shook Kevin , Spence Chris , Elliott Jane , Baulch Helen , Wilson Henry , Pomeroy John W. 
Published: October 2020

AbstractIn cold agricultural regions, seasonal snowmelt over frozen soils provides the primary source of runoff and transports large nutrient loads downstream. The postglacial landscape of the Canadian Prairies and Northern Plains of the United States creates challenges for hydrological and water quality modeling. Here, the application of conventional hydrological models is problematic because of cold regions hydrological and chemical processes, the lack of fluvially eroded drainage systems, large noncontributing areas to streamflow and level topography. A new hydrodynamic model was developed to diagnose overland flow from snowmelt in this situation. The model was used to calculate the effect of variable contributing areas on (1) hydrological connectivity and the development of (2) tipping points in streamflow generation and (3) predominant chemical transport pathways. The agricultural Steppler Basin in Manitoba, Canada, was used to evaluate the model and diagnose snowmelt runoff. Relationships were established between contributing area and (1) snowmelt runoff intensity, (2) seasonal snowmelt volumes and duration, and (3) inundated, active and connected areas. Variations in the contributing area depended on terrain and snowmelt characteristics including wind redistribution of snow. Predictors of hydrological response and the size of the contributing area were developed which can be used in larger scale hydrological models of similar regions
A Simplified Model for the Phase Composition Curve of Saline Soils Considering the Second Phase Transition
Authors: Wang Liyang , Liu Jiankun , Yu Xinbao , Wang Tengfei , Feng Ruiling 
Published: October 2020

AbstractPhase composition curves are the basis for the thermo‐hydraulic analysis of saline soil in cold regions. Two types of phase transition, namely, the water‐ice and solution‐crystal phase transitions, would occur in saline soils due to the variation in temperature and migration of moisture and solute, and the occurrence order of the two types of phase transition is governed by the initial salt and water content. This study proposed a conceptual model for the water phase composition curve in saline soils by simplifying the correlation between pore solution properties and soil grain. This model was subsequently applied to the analysis of phase composition characteristics and explained the occurrence of a second phase transition in saline soil. The model was validated by the experimental data from known soils and showed both good results and ease of use when compared to three previous models. The conceptual model provides a convenient and intuitive way to understand the phase composition characteristics of pore solution in saline soil in cold regions and is also easy to use in the thermo‐hydraulic analysis such as the prediction of unfrozen water content and solute concentration.
The functional analysis of Cullin 7 E3 ubiquitin ligases in cancer
Authors: Shi Le , Du Dongyue , Peng Yunhua , Liu Jiankang , Long Jiangang 
Published: October 2020

AbstractCullin (CUL) proteins have critical roles in development and cancer, however few studies on CUL7 have been reported due to its characteristic molecular structure. CUL7 forms a complex with the ROC1 ring finger protein, and only two F-box proteins Fbxw8 and Fbxw11 have been shown to bind to CUL7. Interestingly, CUL7 can interact with its substrates by forming a novel complex that is independent of these two F-box proteins. The biological implications of CUL-ring ligase 7 (CRL7) suggest that the CRL7 may not only perform a proteolytic function but may also play a non-proteolytic role. Among the existing studied CRL7-based E3 ligases, CUL7 exerts both tumor promotion and suppression in a context-dependent manner. Currently, the mechanism of CUL7 in cancer remains unclear, and no studies have addressed potential therapies targeting CUL7. Consistent with the roles of the various CRL7 adaptors exhibit, targeting CRL7 might be an effective strategy for cancer prevention and treatment. We systematically describe the recent major advances in understanding the role of the CUL7 E3 ligase in cancer and further summarize its potential use in clinical therapy.
Photodynamic therapy induces autophagy-mediated cell death in human colorectal cancer cells via activation of the ROS/JNK signaling pathway
Authors: Song Changfeng , Xu Wen , Wu Hongkun , Wang Xiaotong , Gong Qianyi , Liu Chang , Liu Jianwen , Zhou Lin 
Published: October 2020

AbstractEvidence has shown that m-THPC and verteporfin (VP) are promising sensitizers in photodynamic therapy (PDT). In addition, autophagy can act as a tumor suppressor or a tumor promoter depending on the photosensitizer (PS) and the cancer cell type. However, the role of autophagy in m-THPC- and VP-mediated PDT in in vitro and in vivo models of human colorectal cancer (CRC) has not been reported. In this study, m-THPC-PDT or VP-PDT exhibited significant phototoxicity, inhibited proliferation, and induced the generation of large amounts of reactive oxygen species (ROS) in CRC cells. From immunoblotting, fluorescence image analysis, and transmission electron microscopy, we found extensive autophagic activation induced by ROS in cells. In addition, m-THPC-PDT or VP-PDT treatment significantly induced apoptosis in CRC cells. Interestingly, the inhibition of m-THPC-PDT-induced autophagy by knockdown of ATG5 or ATG7 substantially inhibited the apoptosis of CRC cells. Moreover, m-THPC-PDT treatment inhibited tumorigenesis of subcutaneous HCT116 xenografts. Meanwhile, antioxidant treatment markedly inhibited autophagy and apoptosis induced by PDT in CRC cells by inactivating JNK signaling. In conclusion, inhibition of autophagy can remarkably alleviate PDT-mediated anticancer efficiency in CRC cells via inactivation of the ROS/JNK signaling pathway. Our study provides evidence for the therapeutic application of m-THPC and VP in CRC.
RING-finger protein 166 plays a novel pro-apoptotic role in neurotoxin-induced neurodegeneration via ubiquitination of XIAP
Authors: Oh Chang-Ki , Choi Young Ki , Hwang Ih-Yeon , Ko Yeon Uk , Chung In Kwon , Yun Nuri , Oh Young J. 
Published: October 2020

AbstractThe dopaminergic neurotoxin, 6-hydroxydopamine (6-OHDA), has been widely utilized to establish experimental models of Parkinson disease and to reveal the critical molecules and pathway underlying neuronal death. The profile of gene expression changes following 6-OHDA treatment of MN9D dopaminergic neuronal cells was investigated using a TwinChip Mouse-7.4K microarray. Functional clustering of altered sets of genes identified RING-finger protein 166 (RNF166). RNF166 is composed of an N-terminal RING domain and C-terminal ubiquitin interaction motif. RNF166 localized in the cytosol and nucleus. At the tissue level, RNF166 was widely expressed in the central nervous system and peripheral organs. In the cerebral cortex, its expression decreased over time. In certain conditions, overexpression of RNF166 accelerates the naturally occurring neuronal death and 6-OHDA–induced MN9D cell death as determined by TUNEL and annexin-V staining, and caspase activation. Consequently, 6-OHDA–induced apoptotic cell death was attenuated in RNF166-knockdown cells. In an attempt to elucidate the mechanism underlying this pro-apoptotic activity, binding protein profiles were assessed using the yeast two-hybrid system. Among several potential binding candidates, RNF166 was shown to interact with the cytoplasmic X-linked inhibitor of apoptosis (XIAP), inducing ubiquitin-dependent degradation of XIAP and eventually accelerating caspase activation following 6-OHDA treatment. RNF166’s interaction with and resulting inhibition of the XIAP anti-caspase activity was further enhanced by XIAP-associated factor-1 (XAF-1). Consequently, depletion of RNF166 suppressed 6-OHDA–induced caspase activation and apoptotic cell death, which was reversed by XIAP knockdown. In summary, our data suggest that RNF166, a novel E3 ligase, plays a pro-apoptotic role via caspase activation in neuronal cells.
BCL-XL is an actionable target for treatment of malignant pleural mesothelioma
Authors: Arulananda Surein , O’Brien Megan , Evangelista Marco , Harris Tiffany J. , Steinohrt Nikita S. , Jenkins Laura J. , Walkiewicz Marzena , O’Donoghue Robert J. J. , Poh Ashleigh R. , Thapa Bibhusal , Williams David S. , Leong Trishe , Mariadason John M. , Li Xia , Cebon Jonathan , Lee Erinna F. , John Thomas , Fairlie W. D. 
Published: October 2020

AbstractDespite having one of the lowest survival rates of all cancers, there have been no new approved treatments for malignant pleural mesothelioma (MPM) in over a decade. Standard-of-care treatment relies on Cisplatin plus Pemetrexed chemotherapy. Here, we tested a suite of BH3-mimetic drugs targeting BCL-2 pro-survival proteins of the intrinsic apoptotic pathway. We found BCL-XL is the dominant pro-survival protein in a panel of cell lines in vitro, though potent, synergistic cell killing occurred with MCL-1 co-targeting. This correlates with high-level expression of BCL-XL and MCL-1 in cell lines and a large cohort of patient tumour samples. BCL-XL inhibition combined with Cisplatin also enhanced cell killing. In vivo BCL-XL inhibition was as effective as Cisplatin, and the combination enhanced tumour growth control and survival. Genetic ablation of MCL-1 also enhanced the effects of BCL-XL inhibitors, in vivo. Combined, these data provide a compelling rationale for the clinical investigation of BH3-mimetics targeting BCL-XL in MPM.
Interstellar anatomy of the TeV gamma-ray peak in the IC443 supernova remnant
Authors: Dell’Ova P. , Gusdorf A. , Gerin M. , Riquelme D. , Güsten R. , Noriega-Crespo A. , Tram L. N. , Houde M. , Guillard P. , Lehmann A. , Lesaffre P. , Louvet F. , Marcowith A. , Padovani M. 
Published: October 2020

Context.Supernova remnants (SNRs) represent a major feedback source from stars in the interstellar medium of galaxies. During the latest stage of supernova explosions, shock waves produced by the initial blast modify the chemistry of gas and dust, inject kinetic energy into the surroundings, and may alter star formation characteristics. Simultaneously,γ-ray emission is generated by the interaction between the ambient medium and cosmic rays (CRs), including those accelerated in the early stages of the explosion.Aims.We study the stellar and interstellar contents of IC443, an evolved shell-type SNR at a distance of 1.9 kpc with an estimated age of 30 kyr. We aim to measure the mass of the gas and characterize the nature of infrared point sources within the extended G region, which corresponds to the peak ofγ-ray emission detected by VERITAS andFermi.Methods.We performed 10′ × 10′ mapped observations of12CO,13COJ= 1–0,J= 2–1, andJ= 3–2 pure rotational lines, as well as C18OJ= 1–0 andJ= 2–1 obtained with the IRAM 30 m and APEX telescopes over the extent of theγ-ray peak to reveal the molecular structure of the region. We first compared our data with local thermodynamic equilibrium models. We estimated the optical depth of each line from the emission of the isotopologs13CO and C18O. We used the population diagram and large velocity gradient assumption to measure the column density, mass, and kinetic temperature of the gas using12CO and13CO lines. We used complementary data (stars, gas, and dust at multiple wavelengths) and infrared point source catalogs to search for protostar candidates.Results.Our observations reveal four molecular structures: a shocked molecular clump associated with emission lines extending between −31 and 16 km s−1, a quiescent, dark cloudlet associated with a line width of ~2 km s−1, a narrow ring-like structure associated with a line width of ~1.5 km s−1, and a shocked knot. We measured a total mass of ~230, ~90, ~210, and ~4M⊙, respectively, for the cloudlet, ring-like structure, shocked clump, and shocked knot. We measured a mass of ~1100M⊙throughout the rest of the field of observations where an ambient cloud is detected. We found 144 protostar candidates in the region.Conclusions.Our results emphasize how the mass associated with the ring-like structure and the cloudlet cannot be overlooked when quantifying the interaction of CRs with the dense local medium. Additionally, the presence of numerous possible protostars in the region might represent a fresh source of CRs, which must also be taken into account in the interpretation ofγ-ray observationsin this region.
Can supernova shells feed supermassive black holes in galactic nuclei?
Authors: Palouš J. , Ehlerová S. , Wünsch R. , Morris M. R. 
Published: October 2020

Aims. We simulate shells created by supernovae expanding into the interstellar medium of the nuclear region of a galaxy, and analyze how the shell evolution is influenced by the supernova position relative to the galactic center, by the interstellar matter density, and by the combined gravitational pull of the nuclear star cluster and supermassive black hole (SMBH).
Methods. We adopted simplified hydrodynamical simulations using the infinitesimally thin layer approximation in 3D (code RING) and determined whether and where the shell expansion may bring new gas into the inner parsec around the SMBH.
Results. The simulations show that supernovae occurring within a conical region around the rotational axis of the galaxy can feed the central accretion disk surrounding the SMBH. For ambient densities between 103 and 105 cm−3, the average mass deposited into the central parsec by individual supernovae varies between 10 and 1000 solar masses depending on the ambient density and the spatial distribution of supernova events. Supernovae occurring in the aftermath of a starburst event near a galactic center can supply two to three orders of magnitude more mass into the central parsec, depending on the magnitude of the starburst. The deposited mass typically encounters and joins an accretion disk. The fate of that mass is then divided between the growth of the SMBH and an energetically driven outflow from the disk.
Slow and sausage loop mode excitation due to local and global spontaneous perturbations
Authors: Capettini H. , Cécere M. , Costa A. , Krause G. , Reula O. 
Published: October 2020

Aims. We analyse the capability of different types of perturbations associated with usual environment energy fluctuations of the solar corona to excite slow and sausage modes in solar flaring loops.
Methods. We performed numerical simulations of magnetohydrodynamic ideal equations with a consideration of straight plasma magnetic tubes subject to local and global energy depositions.
Results. We find that local loop energy depositions of typical microflares [∼(1027 − 1030) erg] are prone to driving slow shock waves that induce slow-mode patterns. The slow-mode features are obtained for every tested local energy deposition inside the loop. Meanwhile, in order to obtain an observable sausage mode pattern, a global perturbation that is capable of instantaneously modifying the internal loop temperature is required; specifically, the characteristic conductive heating time must be much smaller than the radiative cooling one. Experiments carried out by varying the parameter β demonstrate that the excitation of sausage modes does not significantly depend on the value of this parameter but, rather, depends on the global or local character of the energy source.
Susceptibility of Incisional Site Staphylococcus spp. to Three Common Perioperative Antimicrobials in Dogs Undergoing Clean Orthopaedic Procedures
Authors: Chou Po-Yen , Robinson Duane , Kapatkin Amy S. , Epstein Steven E. , Giuffrida Michelle A. , Byrne Barbara A. 
Published: October 2020

AbstractObjectives The aim of this study was to evaluate the susceptibility pattern of the incisional site Staphylococcus spp. to three commonly used perioperative antimicrobials in dogs.Materials and Methods A cohort of client-owned dogs was included in this prospective observational study. After induction of general anaesthesia and hair clipping, culture samples were collected from the incisional site of healthy dogs before orthopaedic surgery. The isolated colonies were identified. The susceptibility pattern of staphylococcal isolates was determined using Clinical and Laboratory Standard Institute standards. The staphylococcal susceptibilities to amoxicillin/clavulanic acid and cefpodoxime were compared with cefazolin using two-sided equality of proportions test.Results A total of 391 bacterial isolates were identified in 115 dogs. Bacillus spp. (n = 200) and Staphylococcus spp. (n = 95) were the most common bacteria isolated. Coagulase- positive and -negative staphylococci were isolated in 44 and 38% of dogs respectively. Cefazolin, amoxicillin/clavulanic acid and cefpodoxime staphylococcal susceptibilities were 68, 77 and 76% respectively. There were no significant differences in Staphylococcus spp. susceptibility between amoxicillin/clavulanic acid or cefpodoxime and cefazolin (p = 0.19, p = 0.25). Thirty-six per cent of coagulase-negative staphylococci and 6% of coagulase-positive staphylococci isolated were methicillin resistant.Conclusion Cefazolin, amoxicillin/clavulanic acid and cefpodoxime had similar susceptibilities for staphylococcal isolates. Routine use of amoxicillin/clavulanic acid or cefpodoxime in replace of cefazolin should be practiced with caution.
A Novel ABC Score Predicts Mortality in Non-ST-Segment Elevation Acute Coronary Syndrome Patients Who underwent Percutaneous Coronary Intervention
Authors: Zheng Ying-Ying , Wu Ting-Ting , Gao Ying , Guo Qian-Qian , Ma Yan-Yan , Zhang Jian-Chao , Xun Yi-Li , Wang Ding-Yu , Pan Ying , Cheng Meng-Die , Song Feng-Hua , Liu Zhi-Yu , Wang Kai , Jiang Li-Zhu , Fan Lei , Yue Xiao-Ting , Bai Yan , Zhang Zeng-Lei , Dai Xin-Ya , Zheng Ru-Jie , Chen You , Ma Xiang , Ma Yi-Tong , Zhang Jin-Ying , Xie Xiang 
Published: October 2020

Abstract
Objective In the present study, we aimed to establish a novel score to predict long-term mortality of non-ST-segment elevation acute coronary syndrome (NSTE-ACS) patients who underwent percutaneous coronary intervention (PCI).
Methods A total of 2,174 NSTE-ACS patients from the CORFCHD-ZZ study were enrolled as the derivation cohort. The validation cohort including 1,808 NSTE-ACS patients were from the CORFCHD-PCI study. Receiver operating characteristic analysis and area under the curve (AUC) evaluation were used to select the candidate variables. The model performance was validated internally and externally. The primary outcome was cardiac mortality (CM). We also explored the model performance for all-cause mortality (ACM).
Results Initially, 28 risk factors were selected and ranked according to their AUC values. Finally, we selected age, N-terminal pro-B-type natriuretic peptide, and creatinine to develop a novel prediction model named “ABC” model. The ABC model had a high discriminatory ability for both CM (C-index: 0.774, p < 0.001) and ACM (C-index: 0.758, p < 0.001) in the derivation cohort. In the validation cohort, the C-index of CM was 0.802 (p < 0.001) and that of ACM was 0.797 (p < 0.001), which suggested good discrimination. In addition, this model had adequate calibration in both the derivation and validation cohorts. Furthermore, the ABC score outperformed the GRACE score to predict mortality in NSTE-ACS patients who underwent PCI.
Conclusion In the present study, we developed and validated a novel model to predict mortality in patients with NSTE-ACS who underwent PCI. This model can be used as a credible tool for risk assessment and management of NSTE-ACS after PCI.
Ophthalmology Clinic for the Homeless in San Francisco: Experiences and Findings
Authors: Nnamani Silva Ogonna N. , Hennein Lauren , Ramanathan Saras 
Published: October 2020

Abstract
Background Homelessness is a growing area of concern for communities around the world and homeless individuals are often not connected to healthcare and, consequently, this population is often left out of crucial studies that influence the care provided at public hospitals. The main aim of this study is to examine the prevalence of eye disease, demographics, and common comorbidities of patients who utilized the student-run, institution-sponsored ophthalmology clinic for homeless individuals across a 2-year period. A secondary aim was to elucidate the role that student-run clinics can play in connecting the homeless population to healthcare and community services.
Methods A retrospective cohort study examined individuals who utilized a student-run, free ophthalmology clinic at two homeless shelters in an urban community for 2 years, from 2017 to 2019.
Results A volunteer sample consisting of 123 residents at two homeless shelters who signed up to receive an eye examination at the free eye clinic. The average age of participants was 51 years, with 74% identifying as male, and the largest number of patients identified as African-American/Black (33%) and White (27%). The most prevalent eye disease was refractive error at a prevalence rate of 70% with presbyopia and myopia accounting for 77% and 42% of the refractive error diagnoses, respectively. A visually significant cataract was the second most prevalent pathology, which was diagnosed in 13% of patients. Diabetic retinopathy had a prevalence rate of 11%. Glaucoma had a prevalence rate of 20%. When stratifying the prevalence of glaucoma by race/ethnicity, Black/African-Americans had a prevalence rate of 30% and Asians had a prevalence rate of 29%.
Conclusion This study demonstrated a high prevalence of eye disease among the homeless population highlighting the major role that social determinants of health play in patient outcomes. Student-run ophthalmology clinics provide rich educational experiences, and most importantly, connect homeless populations to essential ophthalmologic care.