bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proof-of-principle experiment for laser-driven cold neutron source
Authors: Mirfayzi S. R. , Yogo A. , Lan Z. , Ishimoto T. , Iwamoto A. , Nagata M. , Nakai M. , Arikawa Y. , Abe Y. , Golovin D. , Honoki Y. , Mori T. , Okamoto K. , Shokita S. , Neely D. , Fujioka S. , Mima K. , Nishimura H. , Kar S. , Kodama R. 
Published: November 2020

AbstractThe scientific and technical advances continue to support novel discoveries by allowing scientists to acquire new insights into the structure and properties of matter using new tools and sources. Notably, neutrons are among the most valuable sources in providing such a capability. At the Institute of Laser Engineering, Osaka, the first steps are taken towards the development of a table-top laser-driven neutron source, capable of producing a wide range of energies with high brightness and temporal resolution. By employing a pure hydrogen moderator, maintained at cryogenic temperature, a cold neutron ($$\le 25\hbox { meV}$$

≤
25

meV

) flux of $$\sim 2\times 10^3\hbox { n/cm}^2$$

∼
2
×

10
3

n/cm
2

/pulse was measured at the proximity of the moderator exit surface. The beam duration of hundreds of ns to tens of $$\upmu \hbox {s}$$

μ
s

is evaluated for neutron energies ranging from 100s keV down to meV via Monte-Carlo techniques. Presently, with the upcoming J-EPoCH high repetition rate laser at Osaka University, a cold neutron flux in orders of $$\sim 1\times 10^{9}\hbox { n/cm}^2/\hbox {s}$$

∼
1
×

10
9

n/cm
2

/
s

is expected to be delivered at the moderator in a compact beamline.
Lipidomic markers of sperm cryotolerance in cattle
Authors: Evans Holly C. , Dinh Thu T. N. , Ugur Muhammet Rasit , Hitit Mustafa , Sajeev Dishnu , Kaya Abdullah , Topper Einko , Nicodemus Molly C. , Smith Gary D. , Memili Erdogan 
Published: November 2020

AbstractThe objective of the current study was to determine the fatty acid composition of sperm from Holstein bulls with different freezability (Good and Poor;n= 12). Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform–methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography–mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (P< 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18 µg) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9 µg;P< 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P≤ 0.011). These results are significant because they reveal key understandings of fatty acid composition of sperm membrane and lay a foundation for the manipulation of membrane integrity, fluidity, and stability to advance the assisted reproductive technologies.
Clinical factors associated with safety and efficacy in patients receiving direct oral anticoagulants for non-valvular atrial fibrillation
Authors: Yamato Hiroshi , Abe Koichiro , Osumi Shun , Yanagisawa Daisuke , Kodashima Shinya , Asaoka Yoshinari , Konno Kumiko , Kozuma Ken , Yamamoto Takatsugu , Tanaka Atsushi 
Published: November 2020

AbstractAlthough patients suffering from atrial fibrillation have increased worldwide, detailed information about factors associated with bleeding during direct oral anticoagulant therapy remains insufficient. We studied 1086 patients for whom direct oral anticoagulants were initiated for non-valvular atrial fibrillation between April 2011 and June 2017. Endpoints were clinically relevant bleeding or major adverse cardiac and cerebrovascular events until the end of December 2018. Incidences of bleeding and thrombosis were 4.5 per 100 person-years and 4.7 per 100 person-years, respectively. Most bleeding events represented gastrointestinal bleeding. Multivariate analysis revealed initiation of anticoagulants at ≥ 85 years old as significantly associated with bleeding, particularly gastrointestinal bleeding, but not major cardiac and cerebrovascular events. Other significant factors included chronic kidney disease, low-dose aspirin and nonsteroidal anti-inflammatory drugs. For gastrointestinal bleeding alone, histories of gastrointestinal bleeding and malignancy also showed positive correlations, in addition to the above-mentioned factors. Clinicians should pay greater attention to the risk of gastrointestinal bleeding when considering prescription of anticoagulants to patients ≥ 85 years old with atrial fibrillation.
Integrated analysis of microRNA and mRNA expression profiles in Crassostrea gigas to reveal functional miRNA and miRNA-targets regulating shell pigmentation
Authors: Feng Dandan , Li Qi , Yu Hong , Liu Shikai , Kong Lingfeng , Du Shaojun 
Published: November 2020

AbstractMicroRNAs (miRNAs) regulate post-transcription gene expression by targeting genes and play crucial roles in diverse biological processes involving body color formation. However, miRNAs and miRNA-targets underlying shell color polymorphism remain largely unknown in mollusca. Using four shell colors full-sib families of the Pacific oyster Crassostrea gigas, we systematically identified miRNAs and miRNA-targets in the mantles, which organ could produce white, golden, black or partially pigmented shell. RNA sequencing and analysis identified a total of 53 known miRNA and 91 novel miRNAs, 47 of which were detected to differentially express among six pairwise groups. By integrating miRNA and mRNA expression profiles, a total of 870 genes were predicted as targets of differentially expressed miRNAs, mainly involving in biomineralization and pigmentation through functional enrichment. Furthermore, a total of four miRNAs and their target mRNAs were predicted to involve in synthesis of melanin, carotenoid or tetrapyrrole. Of them, lgi-miR-317 and its targets peroxidase and lncRNA TCONS_00951105 are implicated in acting as the competing endogenous RNA to regulate melanogenesis. Our studies revealed the systematic characterization of miRNAs profiles expressed in oyster mantle, which might facilitate understanding the intricate molecular regulation of shell color polymorphism and provide new insights into breeding research in oyster.
Facile modulation the sensitivity of Eu2+/Eu3+-coactivated Li2CaSiO4 phosphors through adjusting spatial mode and doping concentration
Authors: Zhou Luhui , Du Peng , Li Li 
Published: November 2020

AbstractSeries of Eu2+/Eu3+-coactivated Li2CaSiO4 phosphors were prepared by solid-state reaction technique. All the samples emitted the unique emissions of Eu2+ and Eu3+ ions when excited by 395 nm, while the strongest emission intensity was received when x = 0.03. On the basis of theoretical discussion, it is evident that crossover relaxation should be responsible for the thermal quenching mechanism which was further proved by the unchanged lifetime at elevated temperature. Besides, through analyzing the inconsistent responses of the emission intensities of the Eu2+ and Eu3+ ions to the temperature, the optical thermometric properties of the designed phosphors were studied. By selecting different emissions of Eu3+ ions and combining with that of the Eu2+ ions, adjustable sensitivities were realized in the resultant phosphors. Furthermore, the sensitivities of the studied compound were also found to be greatly affected by the doping concentration. The maximum absolute and relative sensitivities of the synthesized compounds were 0.0025 K−1 and 0.289% K−1, respectively. These achieved results implied that the Eu2+/Eu3+-coactivated Li2CaSiO4 phosphors were promising candidates for optical thermometry. Additionally, this work also provided promising methods to modulate the sensitivities of the luminescent compounds by adjusting spatial mode and doping concentration.
Multiple-beam and double-mode staggered double vane travelling wave tube with ultra-wide band
Authors: Zhang Zheng , Ruan Cunjun , Fahad Ayesha Kosar , Zhang Chenyu , Su Yiyang , Wang Pengpeng , He Wenlong 
Published: November 2020

AbstractThis paper presents design, fabrication and cold test of an ultra-wide band travelling wave tube (TWT) with planar alignment multiple pencil beams. The fundamental double-mode of staggered double vane slow wave structure (SDV-SWS) rather than the only one mode are put forward and adopted to match with the same electron beam to increase the bandwidth greatly. Simultaneous planar alignment multiple pencil beam tunnels are designed to improve interaction impedance and then to enhance output power, gain, efficiency, growth rate. The transmission performance of a two-stage 51-period SDV-TWT in G-band with structure attenuator between two sections shows that it indeed has an ultra-wideband performance from 81 to 110 GHz. By using computer numerical control machining, the SDV-SWS was manufactured and a detailed cold test was conducted. Good agreement is found at the wide band, where S21 is above − 5 dB and S11 is below − 10 dB. 3D PIC simulations with double-mode multiple-beam SDV-TWT within total length of 70 mm show that it can get a nearly 2120 W peak output power, a 42.5 dB corresponding gain and a 10.7% electron efficiency at 94 GHz with a 22.1 kV beam voltage and a 3 × 0.15A beam current. The 3 dB bandwidth of our double-mode SDV-TWT can achieve about 29 GHz.
The effect of dragon-kings on the estimation of scaling law parameters
Authors: Cabrera-Arnau Carmen , Bishop Steven R. 
Published: November 2020

AbstractScaling laws are used to model how different quantifiable properties of cities, such as the number of road traffic accidents or average house prices, vary as a function of city population size, with parameters estimated from data. Arcaute et al. raised the issue of whether specific cities with extremely large population sizes, known as dragon-kings, should be considered separately from other smaller cities when estimating the scaling law parameters since the two types of cities tend to display different behaviour. Through the analysis of randomly generated samples, we find that the inclusion of dragon-kings in the scaling analysis does not affect the estimated values for the parameters but only provided that all the data points satisfy the same scaling law. We also analyse randomly generated samples where data corresponding to a particular city deviates from the scaling law followed by the rest of the cities. We then show that deviations corresponding to dragon-king cities have the most significant effect on the estimated values of the scaling parameters. The extent of this effect also depends on which estimation procedure is used. Our results have important implications on the suitability of scaling laws as a model for urban systems.
Purification and biochemical characterization of Hel a 6, a cross-reactive pectate lyase allergen from Sunflower (Helianthus annuus L.) pollen
Authors: Ghosh Nandini , Sircar Gaurab , Asam Claudia , Wolf Martin , Hauser Michael , Saha Sudipto , Ferreira Fatima , Bhattacharya Swati Gupta 
Published: November 2020

AbstractSunflower pollen was reported to contain respiratory allergens responsible for occupational allergy and pollinosis. The present study describes the comprehensive characterization of a major sunflower allergen Hel a 6. Natural Hel a 6 was purified from sunflower pollen by anion exchange and gel filtration chromatography. Hel a 6 reacted with IgE-antibodies from 57% of 39 sunflower-sensitized patient sera suggesting it to be a major allergen. The patients were of Indian origin and suffering from pollinosis and allergic rhinitis. Hel a 6 exhibited allergenic activity by stimulating mediator release from basophils. Monomeric Hel a 6 displayed pectate lyase activity. The effect of various physicochemical parameters such as temperature, pH, and calcium ion on the functional activity of Hel a 6 revealed a stable nature of the protein. Hel a 6 was folded, and its melting curve showed reversible denaturation in which it refolded back to its native conformation from a denatured state. Hel a 6 displayed a high degree of sequence conservation with the pectate lyase allergens from related taxonomic families such as Amb a 1 (67%) and Art v 6 (57%). The IgE-cross reactivity was observed between Hel a 6 and its ragweed and mugwort homologs. The cross-reactivity was further substantiated by the mediator release when Hel a 6-sensitized effector cells were cross-stimulated with Art v 6 and Amb a 1. Several putative B cell epitopes were predicted and mapped on these 3 allergens. Two antigenic regions were found to be commonly shared by these 3 allergens, which could be crucial for cross-reactivity. In conclusion, Hel a 6 serves as a candidate molecule for diagnosis and immunotherapy for weed allergy.
Bacterial composition of nasal discharge in children based on highly accurate 16S rRNA gene sequencing analysis
Authors: Haro Kaoru , Ogawa Midori , Saito Mitsumasa , Kusuhara Koichi , Fukuda Kazumasa 
Published: November 2020

AbstractNasopharyngeal colonization by bacteria is a prerequisite for progression to respiratory disease and an important source of horizontal spread within communities. We aimed to perform quantitative analysis of the bacterial cells and reveal the microbiota of the nasal discharge in children at the species level based on highly accurate 16S rRNA gene sequencing. This study enrolled 40 pediatric patients with rhinorrhea. The bacterial cells in the nasal discharge were counted by epifluorescence microscopic analysis. The microbiota was analyzed by using the 16S rRNA gene clone library sequencing method. We demonstrated that a high abundance (median 2.2 × 107 cells/mL) of bacteria was contained in the nasal discharge of children. Of the 40 samples, 37 (92.5%) were dominated by OTUs corresponding to Haemophilus aegyptius/influenzae, Moraxella catarrhalis/nonliquefaciens, or Streptococcus pneumoniae. These samples showed higher cell abundance and lower alpha diversity than the remaining three samples in which the other bacteria coexisted. In addition, 12 sequences with low homology to type strains were considered as previously unknown bacterial lineages. In conclusion, the nasal discharge of most young children contains a large amount of respiratory pathogens and several unknown bacteria, which could not only cause endogenous infection but also be a source of transmission to others.
Hepatocyte nuclear factor 1 alpha influences pancreatic cancer growth and metastasis
Authors: Subramani Ramadevi , Medel Joshua , Flores Kristina , Perry Courtney , Galvez Adriana , Sandoval Mayra , Rivera Servando , Pedroza Diego A. , Penner Elizabeth , Chitti Mahika , Lakshmanaswamy Rajkumar 
Published: November 2020

AbstractHepatocyte nuclear factor 1 homeobox alpha (HNF1α) is a transcription factor involved in endodermal organogenesis and pancreatic precursor cell differentiation and development. Earlier studies have reported a role for HNF1α in pancreatic ductal adenocarcinoma (PDAC) but it is controversial. The mechanism by which it impacts PDAC is yet to be explored in depth. In this study, using the online databases we observed that HNF1α is upregulated in PDAC, which was also confirmed by our immunohistochemical analysis of PDAC tissue microarray. Silencing HNF1α reduced the proliferative, migratory, invasive and colony forming capabilities of pancreatic cancer cells. Key markers involved in these processes (pPI3K, pAKT, pERK, Bcl2, Zeb, Snail, Slug) were significantly changed in response to alterations in HNF1α expression. On the other hand, overexpression of HNF1α did not induce any significant change in the aggressiveness of pancreatic cancer cells. Our results demonstrate that reduced expression of HNF1α leads to inhibition of pancreatic cancer growth and progression, which indicates that it could be a potential oncogene and target for PDAC.
Reward anticipation selectively boosts encoding of gist for visual objects
Authors: Swirsky Liyana T. , Marinacci Ryan M. , Spaniol Julia 
Published: November 2020

AbstractReward anticipation at encoding enhances later recognition, but it is unknown to what extent different levels of processing at encoding (gist vs. detail) can benefit from reward-related memory enhancement. In the current study, participants (N = 50) performed an incidental encoding task in which they made gist-related or detail-related judgments about pairs of visual objects while in anticipation of high or low reward. Results of a subsequent old/new recognition test revealed a reward-related memory benefit that was specific to objects from pairs encoded in the attention-to-gist condition. These findings are consistent with the theory of long-axis specialization along the human hippocampus, which localizes gist-based memory processes to the anterior hippocampus, a region highly interconnected with the dopaminergic reward network.
Synergistic effect of biological and advanced oxidation process treatment in the biodegradation of Remazol yellow RR dye
Authors: Thanavel Muruganandham , Bankole Paul Olusegun , Selvam Ramu , Govindwar Sanjay Prabhu , Sadasivam Senthil Kumar 
Published: November 2020

AbstractThe current study investigated the efficiency of synergistic biological and Advanced Oxidation Process (AOPs) treatment (B-AOPs) using Aeromonas hydrophila SK16 and AOPs-H2O2 in the removal of Remazol Yellow RR dye. Singly, A. hydrophila and AOPs showed 90 and 63.07% decolourization of Remazol Yellow RR dye (100 mg L−1) at pH 6 and ambient temperature within 9 h respectively. However, the synergistic B-AOPs treatments showed maximum decolorization of Remazol Yellow RR dye within 4 h. Furthermore, the synergistic treatment significantly reduced BOD and COD of the textile wastewater by 84.88 and 82.76% respectively. Increased levels in laccase, tyrosinase, veratryl alcohol oxidase, lignin peroxidase and azo reductase activities further affirmed the role played by enzymes during degradation of the dye. UV–Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), high-performance liquid chromatography (HPLC) and gas chromatography–mass spectroscopy (GC–MS) confirmed the biotransformation of dye. A metabolic pathway was proposed based on enzyme activities and metabolites obtained after GC–MS analysis. Therefore, this study affirmed the efficiency of combined biological and AOPs in the treatment of dyes and textile wastewaters in comparison with other methods.
ATR-FTIR spectroscopy in blood plasma combined with multivariate analysis to detect HIV infection in pregnant women
Authors: Silva Lidiane G. , Péres Ana F. S. , Freitas Daniel L. D. , Morais Camilo L. M. , Martin Francis L. , Crispim Janaina C. O. , Lima Kassio M. G. 
Published: November 2020

AbstractThe primary concern for HIV-infected pregnant women is the vertical transmission that can occur during pregnancy, in the intrauterine period, during labour or even breastfeeding. The risk of vertical transmission can be reduced by early diagnosis. Therefore, it is necessary to develop new methods to detect this virus in a quick and low-cost fashion, as colorimetric assays for HIV detection tend to be laborious and costly. Herein, attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy combined with multivariate analysis was employed to distinguish HIV-infected patients from healthy uninfected controls in a total of 120 blood plasma samples. The best sensitivity (83%) and specificity (92%) values were obtained using the genetic algorithm with linear discriminant analysis (GA-LDA). These good classification results in addition to the potential for high analytical frequency, the low cost and reagent-free nature of this method demonstrate its potential as an alternative tool for HIV screening during pregnancy.
Ex vivo 18F-fluoride uptake and hydroxyapatite deposition in human coronary atherosclerosis
Authors: Moss Alastair J. , Sim Alisia M. , Adamson Philip D. , Seidman Michael A. , Andrews Jack P. M. , Doris Mhairi K. , Shah Anoop S. V. , BouHaidar Ralph , Alcaide-Corral Carlos J. , Williams Michelle C. , Leipsic Jonathon A. , Dweck Marc R. , MacRae Vicky E. , Newby David E. , Tavares Adriana A. S. , Sellers Stephanie L. 
Published: November 2020

AbstractEarly microcalcification is a feature of coronary plaques with an increased propensity to rupture and to cause acute coronary syndromes. In this ex vivo imaging study of coronary artery specimens, the non-invasive imaging radiotracer, 18F-fluoride, was highly selective for hydroxyapatite deposition in atherosclerotic coronary plaque. Specifically, coronary 18F-fluoride uptake had a high signal to noise ratio compared with surrounding myocardium that makes it feasible to identify coronary mineralisation activity. Areas of 18F-fluoride uptake are associated with osteopontin, an inflammation-associated glycophosphoprotein that mediates tissue mineralisation, and Runt-related transcription factor 2, a nuclear protein involved in osteoblastic differentiation. These results suggest that 18F-fluoride is a non-invasive imaging biomarker of active coronary atherosclerotic mineralisation.
High-density lipoprotein’s vascular protective functions in metabolic and cardiovascular disease – could extracellular vesicles be at play?
Authors: Beazer Jack D. , Patanapirunhakit Patamat , Gill Jason M.R. , Graham Delyth , Karlsson Helen , Ljunggren Stefan , Mulder Monique T. , Freeman Dilys J. 
Published: November 2020

AbstractHigh-density lipoprotein (HDL) is a circulating complex of lipids and proteins known primarily for its role in reverse cholesterol transport and consequent protection from atheroma. In spite of this, therapies aimed at increasing HDL concentration do not reduce the risk of cardiovascular disease (CVD), and as such focus has shifted towards other HDL functions protective of vascular health – including vasodilatory, anti-inflammatory, antioxidant and anti-thrombotic actions. It has been demonstrated that in disease states such as CVD and conditions of insulin resistance such as Type 2 diabetes mellitus (T2DM), HDL function is impaired owing to changes in the abundance and function of HDL-associated lipids and proteins, resulting in reduced vascular protection. However, the gold standard density ultracentrifugation technique used in the isolation of HDL also co-isolates extracellular vesicles (EVs). EVs are ubiquitous cell-derived particles with lipid bilayers that carry a number of lipids, proteins and DNA/RNA/miRNAs involved in cell-to-cell communication. EVs transfer their bioactive load through interaction with cell surface receptors, membrane fusion and endocytic pathways, and have been implicated in both cardiovascular and metabolic diseases – both as protective and pathogenic mediators. Given that studies using density ultracentrifugation to isolate HDL also co-isolate EVs, biological effects attributed to HDL may be confounded by EVs. We hypothesise that some of HDL’s vascular protective functions in cardiovascular and metabolic disease may be mediated by EVs. Elucidating the contribution of EVs to HDL functions will provide better understanding of vascular protection and function in conditions of insulin resistance and potentially provide novel therapeutic targets for such diseases.