bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using an iSTEAM project-based learning model for technology senior high school students: Design, development, and evaluation
Authors: Chung Chih-Chao , Huang Shu-Lan , Cheng Yuh-Ming , Lou Shi-Jer 
Published: December 2020

AbstractThe purposes of this study were to integrate imagination and STEAM education to construct a special topic course on wearable devices for pets for technology senior high school students studying electronic science; to explore the impacts of the learning process on students’ imagination, STEAM competences and satisfaction with learning effectiveness; and to investigate the integration of imagination into STEAM education. This study adopted the design research method and took 40 students in the third year of a special topic course in electronic science at a technology senior high school as the research subjects. The students were randomly divided into 8 groups, and teaching activities were carried out for 18 weeks. This study used questionnaire and documentary analysis methods to carry out peer evaluation, pretests, posttests and student self-report surveys to collect qualitative and quantitative data for statistical analysis and cross-validation. In this study, a new integration model of imagination and STEAM is proposed. The design research method was employed to plan the iSTEAM course and design special topic activities about real-life issues so that the degree of integration of imagination into the STEAM special topic course and the quality of the students’ work were moderately positively correlated and highly positively correlated, respectively, with students’ application of their learning from various aspects of iSTEAM, which can significantly enhance their imagination, STEAM competences, and satisfaction with their learning effectiveness. The iSTEAM teaching model can help engineering educators develop and evaluate iSTEAM courses and learning activities and provides new contributions to and research directions for STEAM education.
Incidence and outcomes of chronic total occlusion percutaneous coronary intervention in the Netherlands: data from a nationwide registry
Authors:   the PCI Registration Committee of the Netherlands Heart Registration, van Veelen A. , Claessen B. E. P. M. , Houterman S. , Hoebers L. P. C. , Elias J. , Henriques J. P. S. , Knaapen P. 
Published: December 2020

Abstract
Background
Patients with chronic total coronary occlusions (CTO) are at increased risk for poor clinical outcomes. We aimed to determine the incidence of CTO percutaneous coronary intervention (PCI) and to identify CTO patients at risk for cardiac events in the nationwide Netherlands Heart Registration (NHR).

Methods
We included all PCI procedures with ≥1 CTO registered in the NHR from January 2015 to December 2018, excluding acute interventions. We used multivariable logistic regression of baseline characteristics to calculate the risk for events as odds ratios (OR) with 95% confidence intervals (CI).

Results
Of the PCIs performed during the study period, 6.3% (8,343/133,042) were for CTOs, with the percentage increasing significantly over time from 5.9% in 2015 to 6.6% in 2018 (p < 0.001). Coronary artery bypass grafting <24 h was carried out in 0.3%, and the only significant predictor was diabetes mellitus (OR 2.97, 95% CI 1.04–8.49, p = 0.042). Myocardial infarction (MI) <30 days occurred in 0.5%, and renal insufficiency (i.e. estimated glomerular filtration rate <30 ml/min per 1.73 m2) was identified as an independent predictor (OR 4.70, 95% CI 1.07–20.61, p = 0.040). Among patients undergoing CTO-PCI, 1‑year mortality was 3.7%, and independent predictors included renal insufficiency (OR 5.59, 95% CI 3.25–9.59, p < 0.001), left ventricular ejection fraction <30% (OR 3.43, 95% CI 2.00–5.90, p < 0.001), previous MI (OR 1.62, 95% CI 1.14–2.31, p = 0.007) and age (OR 1.06 per year increment, 95% CI 1.04–1.07, p < 0.001). Target-vessel revascularisation <1 year occurred in 11.3%.

Conclusion
CTO-PCI is still infrequently performed in the Netherlands. The most important predictor of mortality after CTO-PCI was renal insufficiency. Identification of patients at risk may help improve the prognosis of CTO patients in the future.
Liver X receptor α participates in LPS-induced reduction of triglyceride synthesis in bovine mammary epithelial cells
Authors: Wang Jianfa , Lian Shuai , Song Jun , Wang Hai , Zhang Xu , He Xianjing , Hao Dandan , Wu Rui 
Published: December 2020

AbstractLipopolysaccharides (LPS) could induce milk fat depression via regulating the body and blood fat metabolism. However, it is not completely clear how LPS might regulate triglyceride synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were isolated and purified from dairy cow mammary tissue and treated with LPS. The level of triglyceride synthesis, the expression and activity of the liver X receptor α (LXRα), enzymes related to de novo fatty acid synthesis, and the expression of the fatty acid transporters were investigated. We found that LPS decreased the level of triglyceride synthesis via a down-regulation of the transcription, translation, and nuclear translocation level of the LXRα. The results also indicated that the transcription level of the LXRα target genes, sterol regulatory element binding protein 1 (SREBP1), fatty acid synthetase (FAS), acetyl-CoA carboxylase-1 (ACC1), were significantly down-regulated in DCMECs after LPS treatment. Our data may provide new insight into the mechanisms of milk fat depression caused by LPS.
Nest cameras do not affect nest survival in a meadow-nesting shorebird
Authors: SALEWSKI VOLKER , SCHMIDT LUIS 
Published: December 2020

SummaryIdentifying the fate of birds’ nests and the causes of breeding failure is often crucial for the development of conservation strategies for threatened species. However, collecting these data by repeatedly visiting nests might itself contribute to nest failure or bias. To solve this dilemma, automatic cameras have increasingly been used as a time-efficient means for nest monitoring. Here, we consider whether the use of cameras itself may influence hatching success of nests of the Black-tailed GodwitLimosa limosaat two long-term study sites in northern Germany. Annually between 2013 and 2019, cameras were used to monitor godwit nests. In 2014 and 2019, nests were randomly equipped with cameras or not, and nest survival checked independently of the cameras. Nest-survival models indicated that survival probabilities varied between years, sites and with time of the season, but were unaffected by the presence of cameras. Even though predation is the main cause of hatching failure in our study system, we conclude that predators did not learn to associate cameras with food either when the cameras were initially installed or after they had been used for several years. Cameras were thus an effective and non-deleterious tool to collect data for conservation in this case. As other bird species may react differently to cameras at their nests, and as other sets of predators may differ in their ability to associate cameras with food, the effect of cameras on breeding success should be carefully monitored when they are used in a new study system.
Influence of maternal pre-pregnancy nutritional status on offspring anthropometric measurements and body composition in three Brazilian Birth Cohorts
Authors: Dias Mariane da Silva , Matijasevich Alicia , Barros Aluísio JD , Menezes Ana Maria B. , Schneider Bruna Celestino , Hartwig Fernando Pires , Barros Fernando C. , Wehrmeister Fernando C. , Gonçalves Helen , Santos Iná S. , Assunção Maria Cecilia F , Horta Bernardo L. 
Published: December 2020

AbstractObjective:We aimed at evaluating the association of maternal pre-pregnancy nutritional status with offspring anthropometry and body composition. We also evaluated whether these associations were modified by gender, diet and physical activity and mediated by birth weight.Design:Birth cohort study.Setting:Waist circumference was measured with an inextensible tape, and fat and lean mass were measured using dual-energy X-ray absorptiometry. Multiple linear regression was used to adjust for possible confounders and allele score of BMI. We carried out mediation analysis using G-formula.Participants:In 1982, 1993 and 2004, all maternity hospitals in Pelotas (South Brazil) were visited daily and all live births whose families lived in the urban area of the city were evaluated. These subjects have been followed up at different ages.Results:Offspring of obese mothers had on average higher BMI, waist circumference and fat mass index than those of normal weight mothers, and these differences were higher among daughters. The magnitudes of the association were similar in the cohorts, except for height, where the association pattern was not clear. In the 1982 cohort, further adjustment for a BMI allele score had no material influence on the magnitude of the associations. Mediation analyses showed that birth weight captured part of this association.Conclusions:Our findings suggest that maternal pre-pregnancy nutritional status is positively associated with offspring BMI and adiposity in offspring. And this association is higher among daughters whose mother was overweight or obese and, birth weight explains part of this association.
Antioxidant biomarkers and cardiometabolic risk markers in an Aboriginal community in remote Australia: a cross-sectional study
Authors: Knudsen Lenette , Lyons Jasmine G , O’Dea Kerin , Christensen Dirk L , Brimblecombe Julie K 
Published: December 2020

AbstractObjective:High-quality diets, characterised by nutrient-rich foods, are one of the foundations for health and well-being. Indicators of diet quality, antioxidants, are associated with protection against cardiometabolic diseases. The current study explores relationships between plasma antioxidants and cardiometabolic risk among Aboriginal people in Australia.Design:As part of a community-driven health promotion programme, we conducted a cross-sectional study including a health-behaviour questionnaire, plasma antioxidants and cardiometabolic risk markers (anthropometric, blood pressure measurements, fasting glucose, glycated Hb (HbA1c), lipids, C-reactive protein and albumin-creatinine-ratio) continuous and categorised into population-specific cut-offs. Antioxidants (β-carotene,β-cryptoxanthin, lycopene, lutein-zeaxanthin, retinol andα-tocopherol measured using HPLC) were applied to a principal component analysis, which aggregated these into a single component. Linear regression models were applied to investigate associations between the antioxidant component and cardiometabolic risk markers.Setting:Community in a remote area in Northern Territory, Australia.Participants:A total of 324 Aboriginal people, mean age 35·5 (range 15–75) years.Results:Antioxidant component levels were higher among individuals with higher self-reported vegetable intake (P< 0·01), higher among individuals with higher self-reported fruit intake (P= 0·05) and lower among current smokers (P= 0·06). Linear regression revealed an inverse association between the antioxidant component and C-reactive protein (β= –0·01,P< 0·01) after adjusting for confounders.Conclusion:Higher plasma antioxidant levels, indicators of diet quality, were associated with lower levels of high-sensitivity C-reactive protein in this Aboriginal population in remote Australia. This association suggests plasma antioxidants may be protective against inflammation; however, longitudinal studies are needed to examine this potentially protective relationship.
Magnetic Anomaly Map of Shatsky Rise and Its Implications for Oceanic Plateau Formation
Authors: Huang Yanming , Sager William W. , Zhang Jinchang , Tominaga Masako , Greene John , Nakanishi Masao 
Published: December 2020

AbstractShatsky Rise oceanic plateau was emplaced during a period of frequent geomagnetic polarity reversals, allowing reconstruction of its tectonic evolution using magnetic anomalies. Prior studies mainly focused on identifying magnetic isochrons and encountered difficulties in tracing magnetic lineations over high relief. We complied a large magnetic data set over Shatsky Rise and its environs, using 5.5 × 106 data points from 96 geophysical cruises spanning 54 years. The long‐time span and heterogeneity of component data sets made data merger a challenge. Contributions of internal and external fields, and spurious readings were removed during processing. A “backbone” method, using recent GPS‐navigated data as a foundation, was developed to improve the coherency of the data set. The singular characteristic of the new magnetic anomaly map is that linear magnetic anomalies are ubiquitous. In nearly every place where data are dense enough to delineate anomaly trends, the plateau and surrounding crust are characterized by linear anomalies. Discordant anomalies in some areas imply complex tectonics related to triple junction migration and ridge reorientation. Tamu Massif apparently formed along a segment of Pacific‐Farallon spreading ridge that rotated by 90° as a triple junction migrated through the edifice. Ori Massif appears to have formed on the Pacific‐Izanagi ridge between triple junctions. Shirshov Massif contains discordant lineations that may indicate a microplate. The pervasive occurrence of linear magnetic anomalies within Shatsky Rise implies that these volcanic edifices must have formed by spreading analogous to mid‐ocean ridges that formed anomalously thick crust.
Investigating the reaction and substrate preference of indole-3-acetaldehyde dehydrogenase from the plant pathogen Pseudomonas syringae PtoDC3000
Authors: Zhang Kaleena , Lee Josephine S. , Liu Regina , Chan Zita T. , Dawson Trenton J. , De Togni Elisa S. , Edwards Chris T. , Eng Isabel K. , Gao Ashley R. , Goicouria Luis A. , Hall Erin M. , Hu Kelly A. , Huang Katherine , Kizhner Alexander , Kodama Kelsie C. , Lin Andrew Z. , Liu Jennifer Y. , Lu Alan Y. , Peng Owen W. , Ryu Erica P. , Shi Sophia , Sorkin Maria L. , Walker Patricia L. , Wang Grace J. , Xu Mark C. , Yang Rebecca S. , Cascella Barrie , Cruz Wilhelm , Holland Cynthia K. , McClerkin Sheri A. , Kunkel Barbara N. , Lee Soon Goo , Jez Joseph M. 
Published: December 2020

Abstract
Aldehyde dehydrogenases (ALDHs) catalyze the conversion of various aliphatic and aromatic aldehydes into corresponding carboxylic acids. Traditionally considered as housekeeping enzymes, new biochemical roles are being identified for members of ALDH family. Recent work showed that AldA from the plant pathogen Pseudomonas syringae strain PtoDC3000 (PtoDC3000) functions as an indole-3-acetaldehyde dehydrogenase for the synthesis of indole-3-acetic acid (IAA). IAA produced by AldA allows the pathogen to suppress salicylic acid-mediated defenses in the model plant Arabidopsis thaliana. Here we present a biochemical and structural analysis of the AldA indole-3-acetaldehyde dehydrogenase from PtoDC3000. Site-directed mutants targeting the catalytic residues Cys302 and Glu267 resulted in a loss of enzymatic activity. The X-ray crystal structure of the catalytically inactive AldA C302A mutant in complex with IAA and NAD+ showed the cofactor adopting a conformation that differs from the previously reported structure of AldA. These structures suggest that NAD+ undergoes a conformational change during the AldA reaction mechanism similar to that reported for human ALDH. Site-directed mutagenesis of the IAA binding site indicates that changes in the active site surface reduces AldA activity; however, substitution of Phe169 with a tryptophan altered the substrate selectivity of the mutant to prefer octanal. The present study highlights the inherent biochemical versatility of members of the ALDH enzyme superfamily in P. syringae.
Therapie einer Distalbisslage mit mandibulärer Schwenkung
– ein Fallbericht
Authors: Kley Katrin , Jung Britta A. 
Published: December 2020

ZusammenfassungDer vorliegende Artikel beschreibt die Therapie eines jugendlichen Patienten
mit einer skelettalen Klasse II und mandibulärer Schwenkung mittels
funktionskieferorthopädischer Geräte (herausnehmbar und
festsitzende Klasse-II-Mechanik).
Die diagnostische Therapie – Schlüssel zum Erfolg des
CMD-Kieler-Konzeptes dgT
Authors: Fischer-Brandies Helge , Asche Marc-Daniel , Wunderlich Christian 
Published: December 2020

ZusammenfassungZur Behandlung von Patienten mit craniomandibulären Dysfunktionen (CMD)
wurde das interdisziplinäre CMD-Kieler-Konzept einer
diagnostikgesteuerten Therapie (dgT) entwickelt Es beruht auf den über
Jahrzehnte gesammelten Erfahrungen des Kieler CMD-Teams. Im Zentrum des
Therapiekonzeptes steht die diagnostische Therapie, die Elemente der Diagnostik
und der Therapie vereint. Die Anamnese, die Analyse der Okklusion, die
Kiefergelenks-Funktionsanalyse, die Untersuchung des neuromuskulären
Systems und bei Bedarf ein Kiefergelenks-MRT im Rahmen der Anfangsuntersuchung
des Patienten führen zum organspezifischen Befund. Er enthält
jedoch nur unvollständige Angaben darüber, ob die erhobenen
Befunde Beschwerden verursachen, behandelt werden müssen und wie diese
im biologischen System zusammenhängen. Diese Information liefert die
diagnostische Therapie. Wir verstehen darunter die initiale Phase der Behandlung
meist mit Schiene und eine manuelle Therapie über einen Zeitraum von
mindestens 6 Wochen. Aus der kontinuierlichen diagnostischen Beobachtung
resultiert der beschwerde- und therapierelevante Befund. Auf dieser Grundlage
entwickeln die Behandler in enger interdisziplinärer Abstimmung
miteinander die Hypothesen der Pathogenese, die Therapieziele und das
Therapiekonzept. Der Artikel beschreibt die diagnostische Therapie anhand
praktischer Beispiele als zentralen Bestandteil des CMD-Kieler-Konzeptes dgT. So
ist eine zielgerichtete erfolgreiche Behandlung der CMD auch in schwierigen
Fällen möglich.
Effects of a One-week Vacation with Various Activity Programs on Metabolism and Adipokines
Authors: Neumayr Günther , Engler Clemens , Lunger Lukas , Lechleitner Peter 
Published: December 2020

AbstractThis study was conducted as part of a larger study of East Tyrolean health tourism, and investigates the effects of an active seven-day vacation on metabolic parameters and adipokines. Fifty-two healthy vacationers participated in two types of vacation activities (golf vs. Nordic walking or e-biking [nw&eb]). In the former group, 30 subjects played golf for a mean duration of 33.5 h per week; in the NW&EB group, 22 persons performed Nordic walking or e-biking for a mean duration of 14.2 h per week. Metabolic parameters and adipokines, such as leptin, adiponectin, GF-21, irisin, omentin-1, betatrophin, and resistin, were measured one day before and one day after the stay. After one week, only the NW&EB group experienced a significant decrease of 1.0 kg in body weight. Significant changes in HDL-C, FGF-21, irisin, and omentin-1 were seen in the golf group; and in triglycerides, HbA1c, leptin and adiponectin in the NW&EB group. No significant changes in betatrophin or resistin were registered in either group. A seven-day vacation with an activity program for several hours per week causes favorable changes in metabolic parameters and adipokines known to be involved in the pathophysiology of the metabolic syndrome. The changes differed in their magnitude and significance, depending on the type of activity.
The Effect of Age and Activity Level on Stem Subsidence in Total Hip Arthroplasty
Authors: Zak Stephen , Greenbaum Simon , Singh Vivek , Schwarzkopf Ran , Davidovitch Roy I. 
Published: December 2020

AbstractThe average length of hospital stay (LOS) following total hip arthroplasty (THA) is decreasing due to a variety of factors, including rapid patient mobilization. With increased early joint loading, certain populations, such as those with low systemic bone mineral density, may be at an increased risk for early stem instability. The purpose of this study was to determine whether activity level and patient age affect femoral stem stability and early subsidence. We retrospectively reviewed 821 patients who underwent primary cementless THA. Two cohorts were established based on LOS as a proxy for activity: (1) same calendar day discharge (SDD) and (2) multiple day stay (MDS). Descriptive patient characteristics as well as surgical and clinical data including surgical time, stem alignment, and Activity Measure for Post-Acute Care (AM-PAC) scores were collected. Stem subsidence was assessed by comparing immediate postoperative radiographs with follow-up radiographs taken no less than 3 months after surgery (9.79 ± 6.50 months). Our sample population consisted of 255 SDD (31%) and 566 MDS (69%) patients. Stem subsidence rate was significantly lower in the SDD than the MDS group (0.916 vs. 1.80 mm, p < 0.001). Increasing age was associated with a higher rate of stem subsidence (β = 0.823, p < 0.001). Increased LOS was also associated with increased age (odds ratio = 1.092, p < 0.001). Higher postoperative mobility determined by AM-PAC scores was not associated with shorter LOS (p = 0.630). The result of this study showed increased activity level does not increase rate of subsidence in THA patients, which supports the safety and efficacy of expedited recovery programs. Increased age was found to increase stem subsidence regardless of LOS.
The erasure of nature in the discourse of oil production
Authors: Chen Wenge , Bartlett Tom , Peng Huiling 
Published: December 2020

Abstract
This two-part article suggests ways in which elements of antagonistic discourses of the environment might be combined in a hybrid, innovative discourse that appeals to a broad section of the public while advocating for more environmentally sustainable practices in industry. It proposes an enhanced Positive Discourse Analysis (PDA) that not only identifies points of fissure in the hegemonic discourse but also seeks points of convergence that can be articulated with in a hybrid, counter-hegemonic discourse that maximizes its potential for uptake while destabilizing the prevailing discourses at precisely the fissure points identified. Part I explores the theoretical grounding for an enhanced PDA, introduces the research method and then, based on Stibbe (2016), makes an eco-discourse analysis of discourses by Shell Oil Company (SOC), with a focus on their discourse semantic patterns, showcasing how hegemonic groups employ discourse strategies to serve their interests and what ecological effects such discourses may produce. In Part II (Chen et al. 2021), a comparative analysis is conducted on the SOC discourses and the Greenpeace discourses. As well as highlighting the points of antagonism between the two discourses, it attempts to seek out points of convergence between progressive positions in the discourses. Part II also explores the potential fissures in the hegemonic order and discusses the design of alternative discourses thereupon. It is argued that an enhanced PDA which focuses on solutions rather than problems and collaboration rather than resistance forms a route for positive and interventionist orientations to discourse that promote social change.