bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of long noncoding RNA RP11-89K21.1 and RP11-357H14.17 as prognostic signature of endometrial carcinoma via integrated bioinformatics analysis
Authors: Gao Lingling , Nie Xin , Zhang Wenchao , Gou Rui , Hu Yuexin , Qi Yue , Li Xiao , Liu Qing , Liu Juanjuan , Lin Bei 
Published: April 2020

Abstract
Background: Endometrial carcinoma (EC) is one of the most common malignant tumors in gynecology. The potential functions and mechanisms of long noncoding RNAs (lncRNAs) in the occurrence and progression of EC remains unclear. It’s meaningful to explore lncRNAs signature for providing prognostic value of EC. Methods：The differentially expressed lncRNAs and their prognostic values in EC were investigated based on The Cancer Genome Atlas (TCGA) database; the transcriptional factors (TFs), the competing endogenous RNA (ceRNA) mechanism, functional regulatory network and immune infiltration of RP11-89K21.1 and RP11-357H14.17 were further explored by various bioinformatics tools and databases. Results: We first identified high expression of RP11-89K21.1 and RP11-357H14.17 were closely associated with shorten overall survival (OS) and poor prognosis in patients with EC. We also elucidated the networks of transcription factor and co-expression genes associated with RP11-89K21.1 and RP11-357H14.17. Furthermore, the ceRNA network mechanism was successfully constructed through 2 lncRNAs (RP11-89K21.1 and RP11-357H14.1), 11 miRNAs and 183 mRNAs. Functional enrichment analysis revealed that the targeting genes of RP11-89K21.1 and RP11-357H14.17 were strongly associated with microRNAs in cancer, vessel development, growth regulation, growth factor and cell differentiation, and involved in pathways including cytokine-mediated signaling pathway, transmembrane receptor protein tyrosine kinase signaling pathway and apoptotic signaling pathway. Moreover, RP11-89K21.1 and RP11-357H14.17 were correlated with immune infiltration including CD8_T cell, CD4_Tcell, Macrophage and Neutrophil. Conclusions: We demonstrated for the first time that RP11-89K21.1 and RP11-357H14.17 may play crucial roles in the occurrence, development and malignant biological behavior of EC, and can be regarded as potential prognostic biomarkers for EC.
Uniportal versus multiportal video-assisted thoracoscopic surgery for non-small cell lung cancer: a meta-analysis
Authors: Yan Yueren , Huang Qingyuan , Han Han , Zhang Yang , Chen Haiquan 
Published: April 2020

Abstract
Background Uniportal video-assisted thoracoscopic surgery (U-VATS) has recently emerged as an alternative procedure for non-small cell lung cancer (NSCLC); however, whether U-VATS has advantages over multiportal VATS (M-VATS) remains unknown. Methods We performed a systematic review of two databases (Pubmed and Web of Science) to search comparative studies of U-VATS and M-VATS anatomical pulmonary resection for NSCLC. Parameters of continuous variables (operative time, blood loss, number of resected lymph nodes, drainage duration, length of postoperative stay and pain in postoperative day 1(POD1)) or categorical variables (conversion rates) were retrieved to estimate the comparitiveoutcomes. A subgroup analysis stratified by study type (propensity-matched analysis& randomized-controlled trial versus non-propensity matched analysis) was performed. Result A total of 19 studies with 3809 patients were included in this meta-analysis. U-VATS was performed on 1747 patients, whereas the other 2062 patients underwent M-VATS. This meta-analysis showed that there was no significant difference in operative time (U-VATS: 146.48±55.07min versus M-VATS: 171.70±79.40min, P=0.81), blood loss (74.49±109.03mL versus 95.48±133.67mL, P=0.18), resected lymph nodes (17.28±9.46 versus 18.31±10.17, P=0.62), conversion rate (6.18% versus 4.34%, P=0.14), drainage duration (3.90±2.94 days versus 4.44±3.12 days, p=0.09), length of postoperative stay (6.16±4.40 days versus 6.45±4.80 days, P=0.22), and pain in POD1 (3.94±1.68 versus 3.59±2.76, p=0.07). Subgroup analysis showed the value of PSM&RCT group consistency with overall value. Conclusion This up-to-date meta-analysis shows that the perioperative outcomes of U-VATS and M-VATS anatomical pulmonary resection are equivalent. In addition to minimizing the incisions, thoracic surgeons should pay more emphasize on providing high-quality and personalized surgical care for patients, to improve the survival ultimately.
"Bariatric Families"- A New Phenomenon with Unique Characteristics
Authors: Weiss Netta , Kalamitzky Nataly , Interator Hagar , Lubetzky Ronit , Lev hadar 
Published: April 2020

Abstract
Background: Many obese children have at least one obese parent, and some of them have one parent who had undergone bariatric surgery (“bariatric families”). The perceptions and attitudes towards child obesity of parents in bariatric families vs. non-bariatric families have not been explored. We assessed how parents who underwent bariatric surgery for obesity perceived their child’s obesity compared to those perceptions of obese parents who did not undergo bariatric surgery.Methods: We conducted a cross-sectional survey by interviewing families in which one or both parents underwent bariatric surgery (bariatric group) and comparing their responses to those of families in which one or both parents had been treated conservatively for obesity (control group). The children of both groups were attending the Obesity Clinic of our children’s hospital. Results: Thirty-six children (median age 10.6 years, 18 in each group, matched for age and sex) were recruited. More parents in the bariatric group replied that weight plays an important role in determining self-image (p<0.03), and more replied that their child's obesity is a current and future health problem (p<0.03 and p<0.007, respectively, Table 1). Five children (28%) in the bariatric group had expectations of undergoing bariatric surgery compared to none in the control group (p<0.02), with a similar trend among their parents (44% vs. 11%, respectively, p<0.07). Conclusion: Families in which one or both parents underwent bariatric surgery for obesity revealed different perceptions of their child’s obesity and different opinions about interventions for treating it compared to families with no bariatric surgery.
Distribution pattern and prognosis of metastatic lymph nodes in cervical posterior to level V in nasopharyngeal carcinoma patients
Authors: Jiang Chaoyang , Ma Ji , Gao Hui , Zhang Ling , Li Hua , Zhang Tao , Liu Bisheng 
Published: April 2020

Abstract
Background:Lymph node metastasis in the cervical region posterior to level V (PLV) often occurs in patients with nasopharyngeal carcinoma (NPC), but the significance of lymph node metastasis in this region and the delineation of the radiotherapy target area have not been reported. We aimed to explore the distribution pattern and prognosis of metastatic lymph nodes in the PLV region in patients with NPC.Methods:We retrospectively studied 605 cases of NPC diagnosed by pathology from December 2011 to November 2017. All patients were subjected to an enhanced CT scan from the cranial apex to 2 cm below the sternoclavicular joints. The nodal distribution at each level was assessed in accordance with the Radiation Therapy Oncology Group (RTOG) guidelines proposed in 2013. The central points of the metastatic lymph nodes of the PLV region in the patients were recreated proportionally on the CT images of a standard patient with N0 NPC in reference to the normal anatomy of the PLV area. The correlation between the PLV region and the other levels, the nodal location, and the characteristics and prognosis of the PLV region were analyzed.Results:Lymph node metastasis occurred in 557 (92.06%) of 605 patients, and the top four areas with the highest probability of metastasis were level IIb (77.85%), level VIIa (73.05%), level IIa (60.0%), and level III (41.48%). There were 30 patients (4.95%) with lymph node metastasis in the PLV region. A total of 49 metastatic lymph nodes from the PLV region were counted, and the mean distance of the central point of each lymph node from the leading edge of the trapezius muscle was 14 mm. Linear regression correlation analysis suggested that lymph node metastasis in the PLV region was associated with ipsilateral level IVa (P=0.018), level Va, level Vb, and level Vc lymph node metastasis (all P<0.001). The 5-year OS, PFS, LRFS, and DMFS of 29 patients with lymph node metastasis in the PLV region were 41.6%, 27.7%, 89.1%, and 47.3%, respectively. The 5-year OS, PFS, LRFS, and DMFS of patients with N3 NPC with or without lymph node metastasis in the PLV region were 41.8% vs 67.3% (P=0.007), 27.8% vs 48.5% (P=0.005), 92.3% vs 80.5% (P=0.521), 40.6% vs 78.4% (P<0.001), respectively. Multivariate analysis showed that lymph node metastasis in the PLV region was an independent prognostic factor for DMFS (P<0.05).Conclusion:NPC patients with lymph node metastasis in the PLV region had a poor prognosis and a high risk of distant metastasis. When patients have lymph node metastasis in levels IVa and V, the posterior boundary of the radiotherapy target in ipsilateral level V should be appropriately moved backward. We recommend that the margin of the PLV region may be a new cervical lymph node segment for head and neck tumors.
Approach to develop a climate change resilience assessment framework
Authors: Cardoso M. A. , Brito R. S. , Almeida M. C. 
Published: April 2020

Abstract
Urban areas are complex, vulnerable and continuously evolving, with interacting strategic services, assets and stakeholders. Potential effects of climate dynamics on urban areas may include the aggravation of current conditions, with identification of new hazards or risk drivers. These challenges require an integrated and forward-looking approach to sustainable urban development. Several tools and frameworks for assessing resilience have already been developed in different fields of study. However, aiming to focus on climate change, urban services and infrastructure, some specific needs were identified. In this light, a resilience assessment framework was developed to direct and facilitate an objective-driven resilience diagnosis of urban cities and services; to support decisions on selection of resilience measures and development of strategies to enhance resilience to climate change; to outline a path to co-build resilience action plans; and to track the progress of resilience in the city or in the service over time. The paper presents an outline of the structure of the framework and details the approach used in its development, including engagement tools and actions undertaken to assure stakeholder involvement in its development, validation and testing.
Spatiotemporal distributions and ecological risk assessment of pharmaceuticals and personal care products in groundwater in North China
Authors: Wu Jin , Liu Jingchao , Pan Zenghui , Wang Boxin , Zhang Dasheng 
Published: April 2020

Abstract
The contamination of surface water by pharmaceuticals and personal care products (PPCPs) has attracted widespread attention, but data regarding their impacts on groundwater (GW) are sparse. In river–GW interaction areas, rivers are likely an important source of PPCPs in aquifers, especially rivers impacted by sewage treatment plant effluent. Understanding the characterization, transport, and risk is valuable for the effective protection of vital aquatic ecosystem services, environmental health, and drinking water supplies. To attain this objective, statistics with spatial analysis and ecological risk were used to assess the effects of artificial recharge (AR) engineering on 16 PPCPs in aquifers in North China. The results indicated that 15 PPCPs were detected in unconfined and confined aquifers, with a few PPCPs being detected up to 1,000 ng/L. The most frequently detected PPCPs were sulfisoxazole, sulfachloropyridazine, sulfamerazine, sulfamethazine, sulfamethoxazole, and ibuprofen. In addition, the spatial and seasonal variations in most PPCPs were significant. Furthermore, the maximum concentrations were compared to the predicted no-effect concentrations to evaluate the ecological risk, and four PPCPs were found to be of medium or high ecological risk. This study highlights that AR engineering has a significant ecological effect on GW.
Determination of river design discharge (Tar River case study)
Authors: Sharifi Mohammad , Majdzadeh Tabatabai Mohammad Reza , Ghoreishi Najafabadi Seyed Hossein 
Published: April 2020

Abstract
Rivers are usually exposed to floods that cause significant human and financial loss, for which structures are considered in the rivers for preventing floods and reducing damage. In this way, it is necessary to acquire design discharge for building these structures. The case study was conducted on the Tar River of Ghazi Station in Tehran Province, which originates from 1 km west of Tar Lake in 13 km east of Damavand. The Tar River is one of the tributaries of Damavand River. In this study, the cross section of the river was first measured through surveying and existing maps. The design discharge of flood control structures was designed using the methods described in this study. Moreover, the stage-discharge table was used to obtain the Manning roughness coefficient. The effective discharge was calculated using the sediment rating curve and sediment frequency histogram, while the bankfull discharge was derived from the return period of 17 years discharge record and fitting of Gumbel distribution to the data. The results indicated that the average of dominant, effective, and bankfull discharges could be the appropriate design discharge for the river, as their values are significantly close to each other. However, there is no need to use flood control structures in this river, due to the occurrence of medium frequent flood events in the river.
Evaluation of environmental impacts for future influent scenarios using a model-based approach
Authors: Andersson S. , Rahmberg M. , Nilsson Å. , Grundestam C. , Saagi R. , Lindblom E. 
Published: April 2020

Abstract
Changes in dilution of wastewater to a treatment plant due to infiltration or surface runoff can have a great impact on treatment process performance. This paper presents a model-based approach in which realistic influent scenarios are generated and used as inputs to a dynamic plant-wide process model of the wastewater treatment plant. The simulated operation is subsequently evaluated using life-cycle assessment (LCA) to quantify the environmental impacts of the future influent scenarios. The results show that increased infiltration led to higher environmental impact per kg nitrogen removed. The increase in surface runoff had a minor impact.
Influence of a new ornamental species (Spathiphyllum blandum) on the removal of COD, nitrogen, phosphorus and fecal coliforms: a mesocosm wetland study with PET and tezontle substrates
Authors: Sandoval Luis , Zurita Florentina , Del Ángel-Coronel Oscar Andrés , Adame-García Jacel , Marín-Muñíz José Luis 
Published: April 2020

AbstractThe objective of this study was to evaluate the influence of a new species of plant (Spathiphyllum blandum) in the elimination of chemical oxygen demand (COD), nitrogen, phosphorus and fecal coliforms (FCs) in mesocosms of wetlands with polyethylene terephthalate (PET) and tezontle substrates under a tropical climate. The experiments were developed at the mesocosm level in 20 experimental units; 10 were planted with Spathiphyllum blandum, five in PET substrates and five in tezontle, and 10 more were used as controls without vegetation, of which five contained tezontle and five contained PET. The systems were fed with contaminated water from the river Sordo, with a hydraulic retention time of 3 days for 12 months; samples were taken in the influent and effluents of the mesocosms every 2 weeks, with the purpose of evaluating the removal of contaminants. The results showed that presence of this species tended to improve or significantly improved the removal of COD, NH4-N, PO4-P, and FCs by 7%, 16%, 29% and 12%, respectively. It was also possible to confirm that the presence of this species reduced the rate of denitrification. These results confirm that in developing countries it is feasible to find new wetland species to be used for wastewater phytoremediation.
Behavior of Human Umbilical Vein Endothelial Cells on Titanium Surfaces Functionalized with VE-Cadherin Extracellular 1-4 Domains
Authors: Seo Hye-Jin , Lee SuJin , Kim Ji-Eun , Jang Jun-Hyeog 
Published: April 2020

Background:Angiogenesis is essential for the optimal functioning of orthopedic medical implants. Protein functionalization of implant surfaces can improve tissue integration through proper vascularization and prevent implant failure in patients lacking sufficient angiogenesis.Objective:The aim of this study was to evaluate the angiogenic activity of titanium surfaces functionalized with recombinant VE-cadherin extracelluar1-4 (VE-CADEC1-4) protein in human umbilical vein endothelial cells (HUVECs).Methods:After titanium discs were coated with recombinant VE-CADEC1-4 protein at appropriate concentrations, the behavior of HUVECs on the VE-CADEC1-4-functionalized titanium discs were evaluated by cell adhesion assay, proliferation assay, and real-time RT-PCR.Results:Recombinant VE-CADEC1-4–functionalized titanium surfaces improved the adhesion of HUVECs by 1.8-fold at the optimal concentration, and the proliferative activity was 1.3-fold higher than the control at 14 days. In addition, when angiogenesis markers were confirmed by real-time RT-PCR, PECAM-1 increased approximately 1.2-fold, TEK approximately 1.4-fold, KDR approximately 1.6-fold, and Tie-1 approximately 2.1-fold compared to the control.Conclusions:Recombinant VE-CADEC1-4–functionalized titanium surfaces improved cell adhesion, proliferation, and angiogenic differentiation of HUVECs, suggesting that the VE-CADEC1-4-functionalization of titanium surfaces can offer angiogenic surfaces with the potential to improve bone healing in orthopedic applications.
Establishment of Method for the Determination of Aggregated α-Synuclein in DLB Patient Using RT-QuIC Assay
Authors: Park Seok-Joo , Lee Yun-Jung , Park Jeong-Ho , Jin Hyoung-Tae , Choi Myoung-Ju , Jung Cha-Gyun , Akatsu Hiroyasu , Choi Eun-Kyoung , Kim Yong-Sun 
Published: April 2020

Background:
The accumulation of aggregated α-synuclein (αSyn) is known as one of
the critical reasons to exhibit their variable molecular pathologies and phenotypes in synucleinopathies.
Recent studies suggested that the real-time quaking-induced conversion (RT-QuIC) assay
is one of the potential methods to detect these αSyn aggregates and could detect the aggregated
αSyn in the brain tissue and cerebrospinal fluid (CSF) using the propensity of the prion-like oligomerization.

Objective:
We tried to optimize the αSyn RT-QuIC assay based on the aggregation of αSyn in
brain samples of synucleinopathies by comparing the conditions of the recently reported αSyn RTQuIC
assays.

Methods:
This study applied a highly sensitive RT-QuIC assay using recombinant αSyn (rαSyn) to
detect aggregated αSyn in the brain tissue from dementia with Lewy bodies (DLB).

Results:
This study compared αSyn RT-QuIC assays under conditions such as beads, rαSyn as a
substrate, reaction buffers, and fluorescence detectors. We observed that the addition of beads and
the use of 6x His-tagged rαSyn as a substrate help to obtain higher positive responses from αSyn
RT-QuIC assay seeding with brain homogenate (BH) of DLB and phosphate buffer-based reaction
showed higher positive responses than HEPES buffer-based reaction on both fluorescent microplate
readers. We also observed that the DLB BHs gave positive responses within 15–25h, which is
faster high positive responses than recently reported assays.

Conclusion:
This established αSyn RT-QuIC assay will be able to apply to the early clinical diagnosis
of αSyn aggregates-related diseases in various biofluids such as CSF.
Organo-Halogens and their Possible Involvement in Prebiotic Chemistry
Authors: Youssef-Saliba Sparta , Vallée Yannick 
Published: April 2020

:In this review, we examined the possibility that some halogenated organic derivatives were used in the primitive ocean at the beginning of life on Earth. Firstly, we described the existence of extraterrestrial halogenated molecules, then we studied their nonbiological syntheses on the present Earth, especially in volcanic environments. In order to demonstrate the diversity of today’s halogenated biomolecules, representative examples are given and the biosynthesis of some of them is summarized. Finally, we proposed two aspects of the chemistry of halogenated compounds that may have been useful en route to biomolecules, firstly the use of methyl chloride as the first methylation reagent, secondly the synthesis and use of α-chloro-carbonyl derivatives.
Development of Phthalimide-Donepezil Hybrids as Potent Multitarget- Directed Ligands for the Treatment of Alzheimer’s Disease
Authors: Yu Lintao , Shi Jian , Cheng Xinfeng , Wang Keren , Liu Shuang , Liu Wenmin , Sang Zhipei 
Published: April 2020

Background:
Due to the complex etiology of AD, multi-target-directed ligands
(MTDLs), combining two or more distinct pharmacological moieties, have been developed in both
symptomatic and disease-modifying efficiencies and are considered as an effective way for the
treatment of AD.

Methods:
To test their biological activities, including AChE/BChE inhibitory activity and MAOA/
MAO-B inhibitory activity. In addition, molecular modeling studies were performed to afford
insight into the binding mode.

Results:
The results displayed that compound 4c showed the best AChE inhibitory
activity with an IC50 value of 4.2 μM, which was supported by the kinetic study and docking study.
Compound 4c was also a selective MAO-B inhibitor (IC50 = 8.2 μM). Moreover, compound 4c
could cross the blood-brain barrier in vitro.

Conclusion:
Compound 4c deserved to further study as a potential multifunctional agent for the
treatment of Alzheimer’s disease.
Is It Necessary to Drain the Kidney with Gestational Pyelonephritis?
Authors: Shkodkin S. V. 
Published: April 2020

Summary. Pyelonephritis during pregnancy is considered as a complicated urinary tract infection. Many Russian publications recommend drainage of the upper urinary tract in pregnant women with pyelonephritis.Purpose of the study. To evaluate the effectiveness and safety of drainage-free management of patients with pregnant pyelonephritis, as well as the epidemic prevalence of pyelonephritis during pregnancy.Materials and methods. The efficacy of conservative therapy and postural drainage in 162 pregnant women with pyelonephritis over the past 3 years has been retrospectively evaluated.Results. The highest frequency of pyelonephritis during pregnancy was noted in the second trimester. Conservative therapy was effective in most patients. Ureteric stent drainage was performed in 3.7% of patients. Hospitalization period increased in this group reliably.Conclusions. Pyelonephritis during pregnancy cannot be considered obstructive. Drainage-free management of pregnant women with pyelonephritis should be considered as a priority approach.