bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA IMFlnc1 Promotes Porcine Intramuscular Adipocyte Adipogenesis by Sponging miR-199a-5p to Up-regulate CAV-1
Authors: Wang Jing , Chen Mingyue , Chen Junfeng , Ren Qiaoling , Zhang Jiaqing , Cao Hai , Xing Baosong , Pan Chuanying 
Published: November 2020

Abstract
Background: Local Chinese local pig breeds have thinner muscle fiber and higher intramuscular-fat (IMF) content. But its regulation mechanism has not been discussed in-depth. Studies indicated that long non coding RNAs (lncRNAs) play important role in muscle and fat development.Results: The lncRNAs expressional differences in the longissimus dorsi (LD) muscle were identified between Huainan pigs (local Chinese pigs, fat-type, HN) and Large White pigs (lean-type, LW) at 38, 58, and 78 days post conception (dpc). In total, 2131 novel lncRNAs were identified in 18 samples, and 291, 305, and 683 differentially expressed lncRNAs (DELs) were found between these two breeds at three stages, respectively. The mRNAs that co-expressed with these DELs were used for GO and KEGG analysis, and the results showed that muscle development and energy metabolism were more active at 58 dpc in HN, but at 78 dpc in LW pigs. Muscle cell differentiation and myofibril assembly might associated with earlier myogenesis and primary-muscle-fiber assembly in HN, and cell proliferation, insulin, and the MAPK pathway might be contribute to longer proliferation and elevated energy metabolism in LW pigs at 78 dpc. The PI3K/Akt and cAMP pathways were associated with higher IMF deposition in HN. Intramuscular fat deposition-associated long noncoding RNA 1 (IMFlnc1) was selected for functional verification, and results indicated that it regulated the expressional level of caveolin-1 (CAV-1) by acting as competing endogenous RNA (ceRNA) to sponge miR-199a-5p.Conclusions: Our data contributed to understanding the role of lncRNAs in porcine-muscle development and IMF deposition, and provided valuable information for improving pig-meat quality.
patRoon: Open source software platform for environmental mass spectrometry based non-target screening
Authors: Helmus Rick , laak Thomas Ter , Wezel Annemarie van , de Voogt Pim , Schymanski Emma 
Published: November 2020

Abstract
Mass spectrometry based non-target analysis is increasingly adopted in environmental sciences to screen and identify numerous chemicals simultaneously in highly complex samples. However, current data processing software either lack functionality for environmental sciences, solve only part of the workflow, are not openly available and/or are restricted in input data formats. In this paper we present patRoon, a new R based open-source software platform, which provides comprehensive, fully tailored and straightforward non-target analysis workflows. This platform makes the use, evaluation and mixing of well-tested algorithms seamless by harmonizing various common (primarily open) software tools under a consistent interface. In addition, patRoon offers various functionality and strategies to simplify and perform automated processing of complex (environmental) data effectively. patRoon implements several effective optimization strategies to significantly reduce computational times. The ability of patRoon to perform time-efficient and automated non-target data annotation of environmental samples is demonstrated with a simple and reproducible workflow using open-access data of spiked samples from a drinking water treatment plant study. In addition, the ability to easily use, combine and evaluate different algorithms was demonstrated for three commonly used feature finding algorithms. This article, combined with already published works, demonstrate that patRoon helps make comprehensive (environmental) non-target analysis readily accessible to a wider community of researchers.
Assessment of left ventricular systolic function by non-invasive pressure-strain loop area in young male strength athletes
Authors: Hua Shaohua , Li Pengge , Zhang Yonggao , Li Lijin , Chen Yingchun , Li Zhen , Liu Songyan 
Published: November 2020

Abstract
BackgroundThe health of athletes has been recognized as a worldwide public concern with more reported sudden cardiac deaths (SCD). Therefore, early detection of abnormal heart function in athletes can help reduce the risk of exercise. A novel valid non-invasive method to evaluate left ventricular (LV) myocardial work (MW) using LV pressure-strain loop (PSL), was used in this paper to explore LV systolic function in young male strength athletes.Methods 36 professional young male strength athletes (the athlete group) and 32 healthy, age-matched young men (the control group) were involved in the study. The LVMW parameters were calculated as the area of PSL by two-dimensional speckle tracking echocardiography (2D-STE) and peak systolic LV pressure. The differences between two groups of data and the predictive efficacy of MW parameters for LV systolic function were analyzed.Results The athlete group had significantly higher values of global wasted myocardial work (GWW) and peak strain dispersion (PSD) than did the control group (P＜0.05).Global myocardial work index (GWI), global constructive myocardial work (GCW) and global longitudinal strain (GLS) were lower in the athlete group than that in the control group, although statistical significance was not reached (P＞0.05). Due to the proportion of GWW and GCW, statistically significant reduction was found in global myocardial work efficiency (GWE) in the athlete group. Conventional echocardiography parameters were well correlated with GWW and GWE (P＜0.05). The best predictor of LV myocardial contractile performance in the athletes using receiver operating characteristic curve (ROC) was GWE, with the area under ROC (AUC) of 0.733, sensitivity of 83.3% and specificity of 59.4%. ConclusionsSubclinical changes have appeared in the hearts of young male strength athletes after long-term intensive exercise and LVMW parameters by PSL play an important role in the evaluation of athlete’s LV contractile performance.
Assessment of irreducible aspects in developmental hip dysplasia by magnetic resonance imaging
Authors: Jia Huihui , Wang Liang , Chang Yan , Song Yongrui , Liu Yuqi , Zhang Fuyong , Feng Jie , Yang Xiaodong , Sheng Mao 
Published: November 2020

Abstract
Background：The developmental dysplasia of the hip (DDH) can cause a wide range of pathological changes, and often requires surgical treatment. Preoperative evaluation is very important for DDH. We aimed to assess the diagnostic capability of magnetic resonance imaging (MRI) for irreducible aspects preventing hip reduction in DDH. Methods: A total of 39 pediatric patients who received DDH evaluation in pediatric orthopedics from January 2015 to December 2019 were included. The samples included 4 cases of bilateral DDH and 35 cases of unilateral DDH, a total of 43 hip joint samples. All patients underwent surgical treatment, pathological examination and MRI of hip joint.Results: With pathological results or intraoperative findings as the gold standard, the sensitivity and specificity of MRI were 90.3% and 83.3% for the affected labrum, 92% and 83.3% for thickening of the round ligament, 90.0% and 91.3% for atrophy of the iliopsoas muscle, and 100% and 100% for fibrofatty pulvinar tissue and joint effusion, respectively. Conclutions: The MRI showed an extraordinary capability of detecting these irreducible factors and helped surgeon choose the appropriate treatment strategies.
Implementation and factors affecting the nursing process among nurses working in selected government hospital in southwest Ethiopia
Authors: Adasho Zerihun Adraro , Bekele Daniel Mengistu 
Published: November 2020

Abstract
Background: The nursing process was initially adopted from the general system theory, and was developed and implemented in the field of education. There is a demand to implement the nursing process in practical care in every health institution, but the perception remains that it is time-consuming and impractical. If the nursing process is not valued and not used, nurses may continue to intervene on the basis of a medical diagnosis rather than on the basis of a rational nursing Process steps. In any of the steps, oversight or omission can result in less than optimal nursing care. The purpose of this study was to assess implementation and factors affecting the nursing process among nurses working in selected government hospitals in Southwest Ethiopia.Methods: An institution-based cross-sectional descriptive study was conducted from March 10 to April 1, 2015 in three hospitals in southwest Ethiopia using self-administered questionnaires. This study included a total of 138 nurses using simple random sampling. Data were classified, coded and entered into epidemiological information version 3.5.3, and exported to the statistical package for social science version 20 for analysis, descriptive statistics were used to describe the variables, bivariable and multivariable logistic regression were used to see the effect of each variable on the dependent variable.Result: The nursing process was found to be 73.9 percent implemented. Compared to a Bachelor of Science nurses’, the likelihood of implementing the nursing process was less likely among diploma nurses. Nurses working in administratively supported hospitals implemented the nursing process more compared to those without administrative support. The nursing process had been implemented higher by trained nurses compared to untrained nurses.Conclusion: The implementation of nursing process was good where; nearly seven in every ten nurses implemented the nursing process. Low educational qualification, lack of training, and non-supportive hospital administration were predictors of the nursing process implementation. The health service management, in collaboration with Ethiopian nursing/professional associations and international governmental and non-governmental organizations should give continuous on the job professional development education, and develop nursing practice guidelines.
Exploring Factors Influencing Retention of Nurses in a Religious Hospital in Taiwan: A Cross-Sectional Quantitative Study
Authors: Chiao Li-Hua , Wu Chiu-Feng , Tzeng I-Shiang , Teng An-Na , Liao Ru-Wen , Yu Li Ying , Huang Chin Min , Pan Wei-Han , Chen Chu-Yueh , Su Tsai-Tsu 
Published: November 2020

Abstract
Background: Long-term shortages in the nursing workforce and high turnover rates are common in Taiwan medical industry. Little research has investigated the psychological factors associated with retention of nursing staff. However, in practice, religious hospitals often provide education in medicine or the medical humanities to enhance psychological satisfaction. The objective of this study was to explore factors influencing nursing staff’s retention with their work across different levels of demand. A further objective was to investigate whether medical humanities educations were associated with the retention of nursing staff.
Methods: This study used s elf-administrated questionnaires to survey nurses working in northern areas of Taiwan. The questionnaire design was based on the six levels of Maslow's hierarchy of needs. Participation was voluntary and participants signed informed consent documents. Self-administrated questionnaires were administered by contacting a total of 759 participants; 729 questionnaires were returned (response rate 96.04%). A logistic regression analysis was used to estimate the impact of seniority of nursing work on nurses reported intention to stay after adjusting for nurse characteristics (gender and age).
Results: In the Pearson correlation analysis, nurses’ willingness to stay was moderately correlated with “physical needs”, “safety needs”, “love and belonging needs”, and “esteem needs” (r=0.559, P<0.001 ; r=0.533, P<0.001 ; r=0.393, P<0.001 ; r=0.476, P<0.001, respectively). Furthermore, nurses’ willingness to stay was highly correlated with “self-actualization needs”, “behind self-actualization needs” and “medical humanities education relevant” (r=0.707, P<0.001 ; r=0.728, P<0.001 ; r=0.678, P<0.001, respectively). We found that the odds ratio (OR) of retention of nursing staff who worked within 1 year (OR=4.511, P=0.002 ) and 1-3 years (OR=3.248, P=0.003 ) was significantly higher referred to those who worked 5-10 years.
Conclusions: With regard to medical humanities education, we recommend adjusting the training as the compulsory activities under the official programs are inadequate and adjusting the required hours of medical humanities education. Tailoring different educational programs for different groups (especially for the groups of nurses who have worked 3-5 years and 5-10 years in the case study hospital) might improve acceptance by nursing staff
Nothing wrong about change: The adequate choice of the dependent variable and design in prediction of cognitive training success&nbsp;
Authors: Mattes André , Roheger Mandy 
Published: November 2020

Abstract
BackgroundEven though investigating predictors of intervention success (e.g Cognitive Training, CT) is gaining more and more interest in the light of an individualized medicine, results on specific predictors of intervention success in the overall field are mixed and inconsistent due to different and sometimes inappropriate statistical methods used. Therefore, the present paper gives a guidance on the appropriate use of multiple regression analyses to identify predictors of CT and similar non-pharmacological interventions.MethodsWe simulated data based on a predefined true model and ran a series of different analyses to evaluate their performance in retrieving the true model coefficients. The true model consisted of a 2 (between: experimental vs. control group) x 2 (within: pre- vs. post-treatment) design with two continuous predictors, one of which predicted the success in the intervention group and the other did not. In analyzing the data, we considered four commonly used dependent variables (post-test score, absolute change score, relative change score, residual score), five regression models, eight sample sizes, and four levels of reliability.ResultsOur results indicated that a regression model including the investigated predictor, Group (experimental vs. control), pre-test score, and the interaction between the investigated predictor and the Group as predictors, and the absolute change score as the dependent variable seemed most convenient for the given experimental design. Although the pre-test score should be included as a predictor in the regression model for reasons of statistical power, its coefficient should not be interpreted because even if there is no true relationship, a negative and statistically significant regression coefficient commonly emerges.ConclusionEmploying simulation methods, theoretical reasoning, and mathematical derivations, we were able to derive recommendations regarding the analysis of data in one of the most prevalent experimental designs in research on CT and external predictors of CT success. These insights can contribute to the application of considered data analyses in future studies and facilitate cumulative knowledge gain.
Platelet to Lymphocyte Ratio as a Potential Inflammatory Marker Predicting the Effect of Adjuvant Chemotherapy in Stage II Colorectal Cancer Patients
Authors: Fu Yu , Chen Xiaowan , Song Yongxi , Huang Xuanzhang , Chen Quan , Lv Xinger , Gao Peng , Wang Zhenning 
Published: November 2020

Abstract
Background: The effect of adjuvant chemotherapy in stage II colorectal cancer (CRC) patients has been in controversy for a long time. Our study aimed to find an effective inflammatory marker to predict the effect of chemotherapy.Methods: 708 stage II CRC patients in our institution were included. The subpopulation treatment effect pattern plot (STEPP) analysis was used to determine the optimal inflammatory marker and cut-off value. Propensity score matching (PSM) was performed to balance discrepancy between the chemotherapy and non-chemotherapy group. Survival analysis based on overall survival (OS) and cancer-specific survival (CSS) was performed using Kaplan-Meier methods with log-rank test and Cox proportional hazards regression. The restricted mean survival time (RMST) was used to measure treatment effect.Results: Platelet to Lymphocyte Ratio (PLR) was chosen as the optimal marker with a cut-off value of 130 according to STEPP analysis. In overall survival analysis, PLR level was significantly associated with the effect of chemotherapy (interaction p=0.027). In low-PLR subgroup, chemotherapy patients did not have a better OS than non-chemotherapy patients (RMST: 56.0 months vs. 56.0 months, HR: 0.983, 95 % CI: 0.528-1.829). In high-PLR subgroup, chemotherapy patients had a significant better OS than non-chemotherapy patients (RMST: 56.5 months vs. 51.3 months, HR: 0.371, 95% CI: 0.212-0.649). After PSM, PLR level was still associated with the effect of chemotherapy. In cancer-specific survival analysis, PLR level was not significantly associated with the effect of chemotherapy (interaction p=0.116). In low-PLR subgroup, chemotherapy patients did not have a better CSS than non-chemotherapy patients (RMST: 56.0 months vs. 56.0 months, HR: 1.016, 95 % CI: 0.494-2.087). In high-PLR subgroup, chemotherapy patients had a better CSS than non-chemotherapy patients (RMST: 56.5 months vs.51.3 months, HR: 0.371, 95% CI: 0.212-0.649). After PSM, PLR level was not associated with the effect of chemotherapy.Conclusions: PLR is an effective marker to predict the effect of chemotherapy in stage II CRC patients.
Hypofractionated intensity-modulated radiotherapy with concurrent chemotherapy for elderly patients with locally advanced pancreatic carcinoma
Authors: Iwai Takahiro , Yoshimura Michio , Ashida Ryo , Goto Yoko , Kishi Takahiro , Itasaka Satoshi , Shibuya Keiko , Kanai Masashi , Masui Toshihiko , Fukuda Akihisa , Isoda Hiroyoshi , Hiraoka Masahiro , Mizowaki Takashi 
Published: November 2020

Abstract
Background: It is important to understand how elderly patients with locally advanced pancreatic carcinoma (LAPC) should be treated, since the number of elderly cancer patients will increase. However, the optimal treatment for elderly patients with LAPC remains unclear. The purpose of this study was to evaluate the efficacy and safety of hypofractionated intensity-modulated radiotherapy (IMRT) with concurrent gemcitabine for elderly patients with LAPC. Methods: We retrospectively analysed the data from LAPC patients aged ≥75 years treated with hypofractionated IMRT (48 Gy in 15 fractions) with concurrent weekly gemcitabine at our institution from February 2013 to December 2018. Overall survival (OS), progression-free survival (PFS), locoregional progression-free survival (LRPFS), distant metastasis-free survival (DMFS), and the pattern of recurrence and toxicity were analysed.Results: Fifteen patients received treatment during the study period. The median age was 78 years (range, 75-86 years), and the Eastern Cooperative Oncology Group (ECOG) performance status (PS) of all patients was 0-1. The median survival time (MST) and median PFS were 20.4 (95% confidence interval (CI), 10.3-36.8) and 13.5 (95% CI, 6.4-20.3) months, respectively, and the one-year OS and PFS rates were 80.0% (95% CI, 50-93.1%) and 66.7% (95% CI, 37.5-84.6%), respectively. The median LRPFS and median DMFS were 15.6 (95% CI, 6.4-36.8) and 14.9 (95% CI, 7.0-20.5) months, respectively, and the one-year LRPFS and DMFS rates were 73.3% (95% CI, 43.6-89.1%) and 66.7% (95% CI, 37.5-84.6%), respectively. Non-haematologic grade 3 toxicity was observed in three cases, of which only one was induced by radiotherapy, whereas grade 4-5 non-haematologic acute or late toxicities were not observed.Conclusions: The OS and PFS of elderly patients with LAPC treated using hypofractionated IMRT with concurrent gemcitabine were favourable and without the occurrence of severe toxicity. This treatment strategy is feasible and promising for elderly LAPC patients with good PS.
Bronchoscopist's perception of the quality of the single-use bronchoscope (Ambu aScope4 ™) in selected bronchoscopies. A multicenter study in 21 Spanish pulmonology services
Authors: Aldeyturriaga Javier Flandes , Giraldo Luis , Alfayate Javier , Fernández-Navamuel Iker , Agustí Carlos , Lucena Carmen M. , Rosell Antoni , Andreo Felipe , Centeno Carmen , Montero Carmen , Garcia Iria Vidal , Alfonso Lucia García , Bango Antonio , Ariza Miguel , Dominguez Rocio Gallego , Caamaño Marta Orta , Bello Salvador , Mincholé Elisa , Torrego Alfons , Pajares Virginia , González Hector , Pérez Aurelio Luis Wangüemert , Pérez-Izquierdo Julio , Disdier Carlos , Sanchez Blanca de Vega , Cordovilla Rosa , Cascón Juan , Cruz Antonio , García-López J. Javier , Puente Luis , Benedetti Paola , García-Gallo Cristina L. , Nuevo Gema Díaz , Aguado Silvia , Partida Concepcion , Díaz-Agero Prudencio , Crespo Estefania Luque , Masa Maria Pavón , Paez Francisco , Cases Enrique , Martinez Raquel , Briones Andres , Fernandez Cleofe , Serrano Concepcion Martin , Uribe-Hernandez Ana Maria , Robles Jose 
Published: November 2020

Abstract
Background: The disposable bronchoscope is an excellent alternative to face the problem of SARS-CoV-2 and other cross infections, but the bronchoscopist's perception of its quality has not been evaluated. Methods: To evaluate the quality of the Ambu-aScope4 disposable bronchoscope, we carried out a cross-sectional study in 21 Spanish pulmonology services. We use a standardized questionnaire completed by the bronchoscopists at the end of each bronchoscopy. The variables were described with absolute and relative frequencies, measures of central tendency and dispersion depending on their nature. The existence of learning curves was evaluated by CUSUM analysis.Results: The most frequent indications in 300 included bronchoscopies was bronchial aspiration in 69.3% and the median duration of these was 9.1 minutes. The route of entry was nasal in 47.2% and oral in 34.1%. The average score for ease of use, image, and aspiration quality was 80/100. All the planned techniques were performed in 94.9% and the bronchoscopist was satisfied in 96.6% of the bronchoscopies. They highlighted the portability and immediacy of the aScope4TM to start the procedure in 99.3%, the possibility of taking and storing images in 99.3%. The CUSUM analysis showed average scores> 70/100 from the first procedure and from the 9th procedure more than 80% of the scores exceeded the 80/100 score. Conclusions: The aScope4 ™ scored well for ease of use, imaging, and aspiration. We found a learning curve with excellent scores from the 9th procedure. Bronchoscopists highlighted its portability, immediacy of use and the possibility of taking and storing images.