bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feto papiráceo en embarazo gemelar a término obtenido por cesárea
Authors: Alegre Andrade Patricia , Toko Chavez Marcelo 
Published: October 2020

El feto papiráceo es un feto aplanado y momificado, sin cambios externos, se presenta en embarazo gemelar o múltiple, es de causa desconocida. Las causas de muerte ipueden ser fetales, ovulares y materna. Complicaciones: en la madre es coagulación intravascular diseminada y en el feto sobreviviente puede presentar Aplasia Cutánea Csongénita, disgenesia renal y atresia intestinal. Paciente de 42 años, con embarazo de 38 semanas, cesárea iterativa, diabetes gestacional no controlada, se realiza un manejo conservador con ecografías seriadas y control de laboratorio. Para la culminación del embarazo: cesárea bajo anestesia raquídea, recién nacido de sexo masculino, alumbramiento de placenta más feto papiráceo. En conclusión, manejo individual con control ecográfico seriado del feto sobreviviente, realizar ecografía Doppler, medir el volumen del líquido amniótico, perfil biofísico y control de los factores de riesgo modificables materno, para tomar una conducta optima y adecuada para la culminación del embarazo.
Técnica de membrana inducida en la reconstrucción de defectos óseos postraumáticos
Authors: Lara-Villca Renan , Undurraga-Carmona Norberto 
Published: October 2020

La técnica de membrana inducida o Masquelet, es un procedimiento quirúrgico utilizado para tratar pseudoartrosis, osteomielitis difusa con grandes secuestros y defectos óseos.
Objetivos: evaluar los resultados obtenidos con la técnica de Membrana inducida en el tratamiento quirúrgico de defectos óseos postraumático.
Métodos: estudio descriptivo, longitudinal, tipo serie de casos: se reportaron cinco casos de defectos óseos postraumáticos tratados en el Hospital Clínico Viedma, desde enero de 2016 a diciembre del 2017, mediante reconstrucción en dos etapas. En la primera se indujo la formación de membrana con un espaciador de cemento óseo impregnado con antibiótico. En la segunda se reemplazó el espaciador por injerto óseo. Se evaluó el tiempo de consolidación, resultado funcional y se registraron las complicaciones.
Resultados: se incluyeron cinco pacientes (cuatro varones y una mujer), con edad promedio de 31 años (rango: 16-54). Dos casos fueron en la tibia, un caso en el fémur, uno en el astrágalo y uno en el cúbito. Tres de los defectos óseos fueron en longitud mayor o igual a 50 mm, con un promedio de 64 mm; tres de los casos tenían cultivos positivos al inicio del tratamiento y cuatro presentaron consolidación, con un único aporte de injerto óseo. El tiempo promedio para la consolidación fue ocho meses. Un caso presentó persistencia de infección.
Conclusiones: representa una buena alternativa en el manejo de defectos óseos segmentarios, con tasas adecuadas de consolidación, bajas complicaciones, tolerable por el paciente y reproducible en los hospitales de nuestro medio.
Differentiation of Mathematics Examination at Two Levels for Higher Secondary CBSE Students in India: Study on Students’ Satisfaction and Subject Related Perception
Authors: Mahajan Navita , Garg Seema 
Published: October 2020

The Central Board of Secondary Examination announced that Class 10 students will have two levels of mathematics papers from 2020.The name suggested for the two examinations will be Maths- Standard which will be same kind of existing level of examination, and Maths - Basic, the standard that has been designed easier as compared Standard level. No proposed change in syllabus has been suggested, class room deliverables and internal assessment for both the levels of examination would remain the same. The standard level has been designed for the students who desire to pursue with mathematics at higher levels, while the basic level would be for the students who want to drop Mathematics after class ten and pursue other streams of Subjects. This study aims to find the satisfaction level of students with this format adopted for Mathematics and their attitude towards other subjects. The students were found to quite satisfy with the regulating so as teachers and parents. The satisfaction level was found to be quite high among all stake holders as this was found to be facilitating students in their interest areas for pursuing higher studies rather them making them burdened and stressed during Higher Secondary struggling with Mathematics. Not only this, the regulation also gave a greater clarity among students, if they were interested to pursue their higher studies with this or related subjects.
Elecciones 2018 en Brasil: análisis de las imágenes en Facebook de los candidatos en la segunda vuelta
Authors: Reis Longhi Carla , Santos Oliveira Ivanilce 
Published: October 2020

Este artículo tiene como objetivo analisar las imágenes publicadas en las fanpages de Facebook de los candidatos a la segunda vuelta de las elecciones presidenciales en Brasil en el año 2018, Jair Bolsonaro (PSL) y Fernando Haddad (PT), aplicando la metodología de análisis del discurso y categorización de acuerdo con Bardin (1977). Para eso, fueron trabajadas 214 publicaciones de fotos en las dos fanpages, en el período de 7 a 28 de octubre de 2018, respectivamente los días de las votaciones en la primera y segunda vuelta de las elecciones, el escenario del cual emerge la temática en discución. Considerando el desarrollo del item 1, se discuten las intersecciones entre comunicación, internet y control; en el item 2 se abordan las particularidades del voto, de campañas electorales y de comunicación política. En el item 3, se presentan el análisis cualitativo y cuantitativo de los datos recogidos, terminando con las consideraciones finales.
Luque Fajardo y la beatificación de san Ignacio de Loyola (1610): campo cultural e imagen autorialLuque Fajardo and the Beatification of Saint Ignatius of Loyola (1610): Cultural Field and Authorial Image
Authors: Aguilera Tania Padilla 
Published: October 2020

Abstract
The Relación de la fiesta que se hizo en Sevilla a la beatificación del glorioso san Ignacio… (Seville, 1610) is an occasional text that clearly reflects the propaganda mechanisms of the time. Luque Fajardo’s description of the festivals and the event held create the appropriate context for a text that bears many similarities with some of its previous models. However, its unifying character is novel and will serve as a model for subsequent religious celebrations. Precisely because of its particular circumstantial character, an approach from the point of view of the sociology of literature is especially pertinent.
La adquisición del lenguaje y noción de la realidad
Authors: Romero Juan Carlos 
Published: October 2020

El presente ensayo constituye un intento de relación de tres cuerpos teóricos: el constructivismo radical; la biología de la cognición y la teoría pragmática del lenguaje. El propósito es describir el cómo podría darse la adquisición y/o emergencia de los deísticos de tiempo y espacio, que constituyen los componenetes léxicos básicos de la noción de realidad. Con este fin se establecen complementariedades y afinidades teóricas que orientan el análisis epistemológico y de la psicología del lenguaje.
¿Cómo Surge el Lenguaje en el Niño?. Los planteamientos de Piaget, Vygotski y Maturana
Authors: Ibañez S. Nolfa 
Published: October 2020

El trabajo contrasta el planteamiento de Humberto Maturana con los aspectos básicos de las teorías de Jean Piaget y L. S. Vygotski sobre el surgimiento del lenguaje. Se discuten las conceptualizaciones propias de cada teoría con respecto a los fundamentos, factores o circunstancias con los que los autores explican la constitución del lenguaje en el niño. El propósito de esta contrastación es contribuir a la comprensión de la nueva visión sobre el lenguaje como generador de realidades que aporta la biología del conocimiento de H. Maturana, y que permite cambiar la mirada sobre las condiciones de constitucion en el niño del fenómeno que nos hace propiamente humanos, posibilitando una proyección distinta e innovadora al trabajo pedagógico y psicopedagógico en el área del lenguaje y la comunicación.
Desarrollo de una Identidad con Tendencia a la Vergüenza Una mirada desde la Teoría de E. Erikson y las Interacciones Familiares
Authors: Donoso Niemeyer Tania 
Published: October 2020

Este artículo intenta relacionar la resolución de los estadios del desarrollo psicosocial planteados por E. Erikson: Autonomía vs. Vergüenza y Dudae Iniciativa vs. Culpa, con el desarrollo de una identidad saludable o de una identidad con tendencia a la vergüenza (concepto desarrollado por Harper & Hoopes, 1990).En seguida se discuten algunos procesos de interacción familiar que facilitan la estructuración de una identidad con tendencia a la vergüenza en una persona, como también características del funcionamiento familiar que permiten la conformación de una identidad saludable.Finalmente se analiza la función que cumpliría el proceso de diferenciación personal en la posibilidad de cambio desde una identidad con tendencia a la vergüenza hacia una identidad saludable.
Ciudadanía y autocuidado en salud reproductiva y sexual: Un estudio exploratorio en mujeres trabajadoras de los sectores salud y educación
Authors: González Rodriguez Sergio , Arriagada Pincetti Marcela 
Published: October 2020

Se relacionan las temáticas de Ciudadanía y Autocuidado en salud reproductiva y sexual refiriendose un estudio en mujeres que trabajan en las areas de salud y educación con formación universitaria y técnica. A trvez de un encuadre metodológico cualitativo basado en entrevistas en profundidad se analizan las practicas de autocuidado en estas areas y se constatan las carencias determinadas por creencias, contenidos congnitivos - emocionales y relaciones proveedor/usuaria que inhiben su fortalecimiento. Consecuentemente, se establecen derechos ciudadanos en el ámbito de la salud reproductiva, los cuales asegurarias mayores niveles de responsabilidad y de autonomia en el autocuidado, reforzando el establecimiento de ciudadanias activas.
Lost verplichte aanwijzing accountant problemen van beursvennootschappen op?
Authors: Eijkelenboom E.V.A. 
Published: October 2020

Lost verplichte aanwijzing accountant problemen van beursvennootschappen op?
Controleplichtig maar geen accountant kunnen vinden. Het overkomt verschillende beursvennootschappen. Minister Hoekstra is daarom voornemens om een verplichte toewijzing van een accountant aan controleplichtige entiteiten wettelijk te verankeren. In deze bijdrage staan de achtergrond en enkele aandachtspunten bij het voornemen van verplichte aanwijzing van de accountant centraal.
Distribution of isonymy index in the territory of Ukraine
Authors: Gorpynchenko M. Yu. , Atramentova L. A. 
Published: October 2020

Purpose of the study: to calculate the irrationality index Ir for the population of the region level, to analyze the regularity of distribution of its values in the territories of Ukraine, which differ in geographical conditions and previous history of population formation. Methods. Using total names, isonymy indices for the entire population of Ukraine, individual regions and oblasts are calculated. Results. The Ir index for the whole population of Ukraine is 2,13×10-4. In some regions of Ukraine it has the following meanings: east – 2,69×10-4, south - 2,23×10-4, center - 3,15×10-4, north - 3,40×10-4, west – 2,40×10-4. The Ir frequency distribution map for regional populations shows a gradient of this index rising from the southeast to the northwest of the country. The minimum Ir value is observed in the Crimea (1,61×10-4), the maximum in the Volyn region (7,14×10-4). Conclusions. The Ukrainian population isonymy index is of the same order of magnitude as in other European countries. The territorially dependent variation of the isonymy index is explained in the peculiarities of the history and landscape of the area.
Keywords: population, isonymy, surnames, migration, genetic drift.
The role of siRNAs in genome stability maintaining in the bread wheat introgression lines
Authors: Navalikhina A. G. , Antonyuk M. Z. , Ternovska T. K. 
Published: October 2020

Aim. Deviations of the siRNAs levels from the parental ones in the plants with hybrid genomes are associated with the activation of transposable elements (TE). This, in turn, lead to the further genome rearrangements. Introgression lines Triticum aestivum / Amblyopyrum muticum are cytologically stable, however, there are visible signs of genetic and (or) epigenetic restructuring that are still going on. Molecular mechanisms of these processes are the subject of our study. Methods. The levels of siRNAs in the plant lemmas were determined by small RNA-seq. Reads of the small RNA libraries were aligned to the repeats to find siRNA sequences. Results. Introgression lines (ILs) and parental amphidiploid have variable levels of siRNAs regulating MITE and CACTA transposable elements, compared with the parental bread wheat variety. For twelve TE sequences, majority of which are CACTA elements, decrease in the levels of siRNAs in ILs and amphidiploid, compared to the wheat, is statistically significant. Decreased siRNAs levels could lead to the activation of corresponding TE classes. Conclusions. Variation of siRNA levels in ILs and amphidiploid can be the key factor that cause rearrangements in their genomes. These include activation of TEs, changes in DNA methylation patterns, and gene expression variation. Therefore, detected changes in siRNA levels can be the molecular mechanisms of the processes that occur in studied hybrid genomes.
Keywords: siRNA, transposable elements, amphidiploid, introgression lines.
Estimation of NAM-A1 haplotypes effect on the level of quantitative traits and grain protein content in wheat
Authors: Orlovskaya O. A. , Vakula S. I. , Khotyleva L. V. , Kilchevsky A. V. 
Published: October 2020

Aim. NAM-A1 gene plays an important role in the regulation of metabolic processes, the remobilization of nutrients from the wheat vegetative organs to grain. The aim of this work is the genotyping of SNP polymorphisms of the NAM-A1 gene in introgression lines derived from the cross of bread wheat varieties with species of genus Triticum, as well as estimation of NAM-A1 haplotypes effect on quantitative traits and grain protein content of the studied genotypes. Methods. NAM-A1 gene polymorphisms were identified using the TaqMan - Real-time PCR. Statistical data analysis was carried out using MS Excel 2007, Statistica 10.0. Results. NAM-A1c haplotype is mainly found in the studied genus Triticum species, NAM-A1d - in bread wheat varieties, and mixed haplotypes - in the introgression wheat lines. Statistically significant associations among NAM-A1 gene haplotypes and productive tillering of plants, the length of the main spike, the number of spikelets in the main spike and the thousand kernel weight were revealed. Conclusions. A significant effect of the NAM-A1 haplotypes was established for the traits «productive tillering» and «thousand kernel weight». The highest thousand kernel weight was reached in plants carrying the haplotype NAM-A1d.
Keywords: T. aestivum L., species of genus Triticum, NAM-A1, SNP, quantitative characters.
Quantitative description of early transcriptional response of plant cells to radiation-induced DNA damage using a Poisson initiation event model
Authors: Litvinov S. V. , Rashydov N. M. 
Published: October 2020

Aim. One of the problems that have not lost their relevance is the study of the mechanisms of adaptation of higher plants to the effects of radiation associated with the modification of the DNA repair system in response to radiation. This paper presents a Poisson mathematical model of the radiation-induced early transcriptional response of genes of key enzymes, which catalyze recovery of double-stranded DNA breaks in active plant cells. Methods. We used total X-ray irradiation of a model object – 35-day-old Arabidopsis thaliana (L.) Heynh plants at sublethal doses of 3-21 Gy, total RNA extraction, reverse transcription with random hexanucleotide primers, PCR amplification of the obtained cDNA with primers to target genes, fluorescence gel densitometry of amplified products. Results. A mathematical model of transcriptional response to the genotoxic action of ionizing radiation in a subpopulation of active plant cells based on Poisson distribution, which satisfactorily describes the experimental data obtained, is proposed. Conclusions. To initiate a maximal transcriptional response to DNA damage, one two-strand lesion per chromosome, detected by DNA repair systems, is sufficient, while the absence of double-stranded lesions, or the appearance of more than one double-stranded lesion per chromosome inhibits early transcriptional response of the cell on the action of ionizing radiation. The Poisson model of the initiating event makes it possible to predict the response of subpopulations of active cells of angiosperms to the action of genotoxic factors.
Keywords: ionizing radiation, DNA damage response (DDR), Arabidopsis thaliana, DNA repair, gene transcriptional activity.
Synthesis, characterization and antiradiation properties of the tryptophanates of cobalt (II), manganese (II), copper (II) and zinc
Authors: Shamilov E. N. , Abdullaev A. S. , Farajov M. M. , Shamilli V. E. , Gahramanova Sh. I. , Jalaladdinov F. F. 
Published: October 2020

Aim. In order to study the radioprotective activity were were obtained in the tryptophanates of cobalt (II), manganese (II), copper (II) and zinc. Methods. The composition and structure of the complexes were studied by elemental, thermogravimetric analyzes and IR infrared spectroscopy. To determine the presence of the Radioprotective Properties of the Co (II), Mn (II), Cu (II), Zn complexes with tryptophane, a test was conducted for the 30-day survival of irradiated animals. Results. The results of thermogravimetric studies have shown that the final product of the thermal decomposition of all compounds is metal oxide, respectively. The method of IR spectroscopy showed that the ligands in the composition of the metal (II) complexes enter the neutral form and coordinate with the complexing agent through the nitrogen atom. The results of experiments with complexes showed that they have noticeable radioprotective activity. The radioprotective activity of Co (II) complexes with tryptophan is 55%, Mn (II) with tryptophan 50%, Cu (II) with tryptophan 40%, and Zn 30%. They improve the survival and average life expectancy of lethally irradiated mice, not reaching the level of the known cystamine radioprotector, which is 80%. Conclusions. The data obtained by us testify to the prospects of using the complexes of Co (II), Mn (II), Cu (II), Zn with tryptophan for preventive purposes and in order to prevent local radiation injuries.
Keywords: tryptophan-metal complexes, IR spectroscopy, thermogravimetry, complex compounds, radioprotective properties.
Polyploidization of Miscanthus sinensis using dinitroanilines with low phytotoxicity
Authors: Melnychuk O. V. , Ozheredov S. P. , Rakhmetov D. B. , Rakhmetova S. O. , Bayer O. O. , Shysha O. M. , Yemets A. I. , Blume Ya. B. 
Published: October 2020

Aim. The aim of the work was to obtain polyploid lines of Miscanthus sinensis with enhanced biomass productivity using new dinitroaniline compounds. Methods. The work was carried out in in vitro conditions. Aseptic M. sinensis shoots were cultured on shoot multiplication medium containing various compounds of dinitroanilines class. Classic dinitroanilines (oryzalin and trifluralin) were used as a control. Results. When aseptic shoots were cultured on medium supplemented with oryzalin and trifluralin at concentration 10 mM, survival rate of explants was between 23.52 and 44.40%. However, when the medium was supplemented with tested dinitroanilines at the same concentration, survival rate of explants was in the range of 95.0–96.5%. Conclusions. Possibility of new compounds of dinitroanilines class utilization for M. sinensis polyploidization has been studied. In terms of explants survival rate, application of new compounds of dinitroanilines class appeared to be significantly effective than application of classic dinitroanilines.
Keywords: dinitroanilines, in vitro, polyploidization, Miscanthus sinensis.
Genetic transformation of new perspective winter wheat genotypes in vitro
Authors: Slivka L. V. , Dubrovna O. V. 
Published: October 2020

Aim. Optimization of conditions for genetic transformation of new perspective winter wheat genotypes and production of transgenic plants. Methods. Agrobacterium-mediated transformation in vitro culture using callus cultures. Results. The influence of the optical density of cells of agrobacterial suspension, the concentration of the antibiotic cefotaxime, the duration of coculture on the frequency of obtaining kanamycin-resistant regenerants of new winter wheat genotypes by genetic transformation of callus cultures were investigated. By Agrobacterium-mediated transformation of morphogenic calluses of new perspective winter wheat genotypes were obtained plant-regenerants in the genome of which revealed the complete incorporation of a genetic construct containing oat and nptII transgenes. Conclusions. Agrobacterium-mediated transformation of callus cultures of new perspective winter wheat genotypes was optimized, and transgenic plants with the target gene of ornithine-δ-aminotransferase were obtained.
Keywords: Triticum aestivum, Agrobacterium-mediated transformation, callus cultures, ornithine-δ-aminotransferase gene.
Obtaining of the recombinant Rhil7-Cbd fusion protein
Authors: Usenko M. O. , Gorbatiuk O. B. , Okunev O. V. , Irodov D. M. , Koval’chuk M. V. , Kordium V. A. 
Published: October 2020

Aim. The purpose was to obtain the recombinant fusion protein based on the human interleukin-7 (rhIL7) and cellulose binding domain (CBD). Methods. The DNA sequences encoding rhIL-7 and CBD were subcloned into the pET24a(+). Vector containing the 6His-tag sequence for further chromatographic purification of the target protein. The cells of E. coli strain BL21(DE3) were transformed with pET24-rhIL7-CBD plasmid vector. Protein synthesis was induced in clones by IPTG and by autoinduction. Results. An expression cassette of rhIL7-CBD protein has been designed. Producers of rhIL7-CBD protein were obtained. It was shown that the autoinduction protocol provides protein synthesis in E. coli (but IPTG induction doesn’t). Protein was obtained in the cytoplasmic fraction in form of inclusion bodies. In vitro method of purification of rhIL7-CBD from inclusion bodies has been worked out. Conclusions. The obtained rhIL7-CBD protein can be used for the microcrystalline cellulose immunosorbent construction for the purification of the specific polyclonal IL-7 antibodies and also for qualitative and quantitative analysis of IL-7 receptors.
Keywords: IL-7, CBD, inclusion bodies, renaturation.