Free radical scavenging and cytotoxic assay of soursop fruit juice (Annona muricata Linn.) on cervical cancer cell lines (HeLa)
Authors: Widyanto Rahma Micho , Rachmawati Putri Rifanty Meydiana , Kurniasari Fuadiyah Nila , Yunimar Yunimar , Utomo Budi 
Published: August 2020

<p align="center"><strong>ABSTRAK</strong></p><p><strong><em>Latar belakang</em></strong><em>: Kanker serviks merupakan penyakit yang menduduki posisi kedua penyebab kematian pada wanita. Berbagai terapi pendukung mulai dikembangkan, seperti melalui bahan makanan yang dipercaya memiliki efek anti</em><em>-</em><em>kanker. Buah sirsak disebut memiliki kandungan fitokimia seperti Annonaceous acetogenin, flavonoid dan fenol yang bermanfaat sebagai anti</em><em>-</em><em>kanker. </em></p><p><strong><em>Tujuan:</em></strong><em> Penelitian ini dilakukan untuk mengetahui potensi anti</em><em>-</em><em>oksidan dan sitotoksisitas dari sari buah sirsak pada sel HeLa secara in vitro. </em></p><p><strong><em>Metode</em></strong><em>: Penelitian ini dilakukan dengan membuat sari buah sirsak dengan cara diblender kemudian dikeringkan dengan metode freeze-drying, yang kemudian dilanjutkan dengan uji 2,2-diphenyl-1-picrylhydrazyl (DPPH) untuk mengetahui potensi penghambatan radikal bebas, dan potensi sitotoksisitas melalui uji MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difenitetrazolium bromide) assay pada sel kanker serviks HeLa. </em></p><p><strong><em>Hasil</em></strong><em>: Hasil uji aktivitas anti</em><em>-</em><em>oksidan menunjukkan persamaan regresi linier (y=0,0149x – 2,8812) dan nilai</em><em> perhitungan</em><em> IC<sub>50</sub> sari buah sirsak sebesar 3549 μg/mL<strong> </strong>dan hasil uji sitotoksisitas menunjukkan persamaan regresi linier (y=0,0197x + 0,3101) dan nilai</em><em> perhitungan</em><em> IC<sub>50</sub> sari buah sirsak pada sel HeLa sebesar 2522,33 μg/mL.</em></p><p><strong><em>Kesimpulan</em></strong><em>: Sari buah sirsak memiliki aktivitas anti</em><em>-</em><em>oksidan yang sangat rendah dan tidak berpotensi sebagai anti</em><em>-</em><em>kanker terhadap sel HeLa secara in vitro.</em><em></em></p><p> </p><p><strong>KATA KUNCI </strong><strong>:</strong><em> Annona muricata Linn, anti</em><em>-</em><em>oksidan, kanker, sitotoksisitas</em></p><p align="center"><strong> </strong></p><p align="center"><strong>ABSTRACT</strong></p><p><strong><em>Background: </em></strong><em>Cervical cancer is the second leading cause of death in women. Various supporting therapies have been developed, such as through food ingredients which are believed to have anti</em><em>-</em><em>cancer effects. Soursop </em><em>is known to be high</em><em> phytoc</em><em>h</em><em>emical content such as Annonaceous acetogenin, flavonoid and phenols which are useful as anti</em><em>-</em><em>cancer. </em></p><p><strong><em>Objectives:</em></strong><em> This research was conducted to determine the antioxidant and cytotoxic activity of soursop juice on HeLa cell lines.</em></p><p><strong><em>Methods: </em></strong><em>This study started</em><em> by</em><em> making the soursop fruit extract by blending then dried with freeze</em><em>-</em><em>drying method, and then proceed with 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay to determine the potential free radical scavenging activity, cytotoxic potential activity in vitro through MTT (3-(4,5-dimethylthiazol-2-il)-2,5-difenitetrazolium bromide) assay on HeLa cell lines.</em></p><p><strong><em>Results: </em></strong><em>The IC<sub>50</sub> antioxidant activity of soursop fruit extract is 3549 μg/mL with linear regression equation (y=0.0149x – 2.8812) and the IC<sub>50</sub> cytotoxicity test of soursop fruit extracton HeLa was 2522,33 μg/mL with linier regression equation (y=0.0197x + 0.3101).<strong> </strong></em></p><p><strong><em>Conclusion: </em></strong><em>The conclusion in this study is that soursop fruit extract has very low antioxidant activity and has no in vitro potential effect as an anti</em><em>-</em><em>cancer on HeLa cell lines.</em></p><p><em> </em></p><p><strong>KEYWORDS </strong><strong>:</strong><em> Annona muricata Linn, antioxidant, cancer, cytotoxic</em></p><p><em> </em></p>