bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteome-Scale Profiling Reveals MAFG and MAFF as Two Novel Candidate Key Transcription Factors Involved in Palmitic acid Induced Umbilical Vein Endothelial Cells Apoptosis
Authors: Wang Mangyuan , Liu Fen , Fang Binbin , Huo Qiang , Yang Yining 
Published: September 2020

Abstract
Backgrounds: Vascular endothelial cell apoptosis is the first risk factor of atherosclerosis (AS), and it can be induced by high doses of glucose and palmitic acid (PA). The purpose of our study is to use a new generation of high-throughput transcription factors (TFs) detecting method to identify novel candidate key TFs involved in PA-induced vascular endothelial cell apoptosis.Methods: Human umbilical vein endothelial cells (HUVECs) were treated with 0µM PA (control group), 250µM PA (group 1), or 500µM PA (group 2). Candidate TFs among the three groups were determined by significant changes according to t-test, and pathway enrichment, western blot (WB) and RT-qPCR were then performed.Results: Fifty-one TFs showing with significant p value were identified, and 24 TFs with significant p value plus fold change > 2 and with dose-dependence were identified with 12 TFs biologically validated in former studies. Two of the remaining 12 novel TFs, v-maf musculoaponeurotic fibrosarcoma oncogene family protein G (MAFG) and v-maf musculoaponeurotic fibrosarcoma oncogene family protein F (MAFF), were matched to AS known signalling pathways and were validated by WB and RT-qPCR in our study.Conclusions: We identified MAFG and MAFF as novel candidate key TFs in vascular endothelial cell apoptosis, which is the key initial process of AS.
The Use of CT Hounsfield Unit Values to Identify The Bone Quality in Patients With Cervical Degenerative Diseases.
Authors: He Lei , Zhou Fei Fei , Sun Yu , Li Wei-Shi 
Published: September 2020

Abstract
Background It is well known that osteoporosis may lead to the failure of spinal surgery. As the gold standard, dual-energy X-ray absorptiometry (DXA) is used to evaluate the overall bone mineral density (BMD). Previous studies have used CT (Computed tomography) value to evaluate local bone mineral density. The objective of this study was to investigate the application value of cervical CT value in preoperative bone quality evaluation of cervical degenerative diseases. Methods A total of 939 patients who received surgical treatment for cervical degenerative diseases in our center from January 2015 to December 2017 were retrospectively reviewed. The Hounsfield unit (HU) values were measured in middle transverse CT images of the C2–C7 on the picture archiving and communication system (PACS), and the total bone mineral density T-score of L1–L4 was obtained by dual-energy X-ray absorptiometry. The changes in the HU values of C2–C7 were observed, the correlation between the HU value of C2–C7 and the total BMD T-score of L1–L4 was analyzed, and the HU thresholds of C2–C7 for different T-scores (-2.5 < T-score <-1 or T-score ≤2.5, respectively) were identified. Results The HU values of C2–C7 show a decreasing trend. The mean HU value of C2–C7 was 322.52 ± 89.27 HU. The average BMD T-score of L1-L4 was -0.73. The average HU value of C2–C7 was positively correlated with the average BMD T-score of L1–L4 (r = 0.487, P < 0.001). The HU threshold of C2–C7 was 269 HU when -2.5＜T-score＜-1, and it had a sensitivity of 75.7% and a specificity of 59.8% when used for screening for osteopenia; the HU threshold of C2–C7 was 269H U when T-score ≤-2.5, and it had a sensitivity of 63.8% and a specificity of 80.8% when used for screening for osteoporosis. Conclusions The HU values of cervical vertebrae gradually decrease from C2 to C7 in patients undergoing surgical treatment for cervical degenerative disorders. The CT HU value of cervical vertebrae is positively correlated with the BMD T-score provided by lumbar DXA, which is helpful for clinical evaluation of bone quality before surgery.
Brugada Syndrome Inducted Ventricular Fibrillation Successfully Supported With Veno-arterial Extracorporeal Membrane Oxygenation and Continuous Renal Replacement Therapy:A Case Report
Authors: Huang Chengfeng , Huang Shifang , Huang Jiawen , Zhang Xiaoshen , Liu Fanna , Lu Hua , Li Xiaohui , Li Yalan 
Published: September 2020

Abstract
BackgroundThe brugada syndrome（BrS）is an inherited disorder that can lead to sudden death. A young man who experienced sudden cardiac arrest caused by Brs was successfully rescued after 2hour cardiopulmonary resuscitation (CPR). This is a case that has raised concerns in China (more than 200 million people read this news via the internet).Case presentationA 28 years old young man was successfully managed with Veno-arterial ECMO (VA-ECMO) and continuous renal replacement therapy (CRRT) after more than 2 hours CPR. The diagnosis of BrS was identified during ECMO supported, and further ECG screening found two asymptomatic patients. ConclusionBrS can lead to malignant arrhythmia. VA-ECMO is the rescue treatment for patients with cardiac arrest who cannot be cured by conventional resuscitation. VA-ECMO combined with CRRT may help to improve the prognosis of critically ill patients.
Design the Model of Sport Development in Military Hygiene and Cure Branch Staff
Authors: Shirvani Hosein , Bahadori Mohammad Karim , Darvishi Abolfazl 
Published: September 2020

Abstract
Background: Sport, as a phenomenon in many people’s lives in society helps improve their health and well-being, which is very important for any society’s development. Promoting physical activity is one of the most important and effective strategies to reduce the risk of diseases, reduce social deviations and increase the vitality of communities. Methods: The present study was conducted in a mixed method. The statistical population of the study included faculty members and physical education professors of the Military universities, managers, coaches and athletes in the field of military. In the qualitative stage, the factors were identified through interviews with 11 people; in the quantitative stage, to collect the data was used a researcher-made questionnaire obtained from the interview and study of research background. The validity of the questionnaire was assessed by calculating the indicators of Content Validity Ratio and content validity index and for calculate the reliability was used to cornebach's alpha. For data analysis was used of structural equation modeling in format of SPSS20 and AMOS23 software. Results: The results of modeling structural equations showed that the dimensions of management, environment and infrastructure had a positive and significant relationship with the dimension of sport participation and individual dimension was not significant relationship with sport participation.Trial registration: Not applicableConclusion: The results of this research can be used to make more progress in the sports of the Armed Forces personnel and ultimately increase the morale and health of these people.
Does Kashin-beck Disease Present Only Multisite Pain? A Cross-sectional Study of Pain Characteristics in Northwest China
Authors: Wang Dimiao , Younus Mohammad Imran , Xu Chao , Fang Hua , Liu Huan , Guo Xiong 
Published: September 2020

Abstract
BackgroundKashin-Beck Disease (KBD) is an endemic, chronic joint disease. Multisite joint pain is the primary symptom of KBD, which have profound negative effects on individuals and society. However, studies on joint pain characteristics among the KBD population are still limited. The aims of this study were to explore characteristics of joint pain in patients with KBD and determine associated factors with joint pain. MethodsThis cross-sectional study included 167 patients with KBD and 169 patients from the general population with joint pain from Shaanxi Province in northwest China. Subjects were asked about joint pain characteristics and completed the numeric rating scale (NRS), the graded chronic pain scale (GCPS) and the EuroQol (EQ-5D) questionnaire. Differences between groups were determined using Chi-square, Student’s t, Mann–Whitney U and 1-way ANOVA tests.ResultsCompared with the general group, patients with KBD reported a higher number of pain sites (7.2±3.8 vs 3.5± 1.8), a higher frequency of persistent pain (98.8% vs 50.9%), a higher percentage of analgesics usage (89.2% vs 30.7%), a higher pain intensity (73.8±15.2 vs 50.0±20.7) and pain –related disability (61.2±23.3 vs 41.6±23.2), and lower EQ-5D scores (0.34±0.27 vs 0.59±0.16). Among the 167 KBD patients, painful joints were symmetrically distributed between the bilateral limbs; the 5 most frequently reported painful joints for the bilateral sides were the knees (84.8%), ankles (79.2%), wrists (51.2%), shoulders (49.5%) and elbows (47.7%). The most severe pain joint was the knee (NRS:6.6), followed by the ankles (NRS:5.1), the fingers, shoulders and elbows had the similar NRS scores (NRS:4.0). Additionally, KBD patients experienced neuropathic pain to varying degrees. Compared to males, females reported a higher number of total pain sites, higher intensity and lower quality of life. ConclusionsBesides multisite pain, the KBD patients suffered from symmetrical, persistent, and neuropathic pain. Weight-bearing joints (e.g., knee and ankle) were the most painful. These findings will provide scientific basis for establishing joint pain evaluation criteria and future pain intervention strategies for these KBD patients in China.
Selective maternal seeding and rearing environment from birth to weaning shape the developing piglet gut microbiome
Authors: Wei Chen , Mi Jiandui , Ma Jingyun , Jiang Yiming , Deng Li , Lv Ning , Gao Jinming , Cheng Jian , Wu Ruiting , Liang Juan Boo , Wang Yan , Lan Tian , Liao Xin Di 
Published: September 2020

Abstract
Background: The acquisition and development of the mammalian microbiome early in life are critical to establish a healthy host-microbiome symbiosis. The maternal and environmental microbial reservoirs are considered the main sources of microbial communities in newborn mammals. However, the timely relative contribution of various microbial sources to the colonization of the gut microbiota in newborn piglets remains unclear. The aim of this study was to investigate the influence of the sow and delivery environment microbes on nursing piglets from birth through weaning.Results: We longitudinally sampled the microbiota of 20 sow-piglet pairs (three piglets per sow) from multiple body sites and the surrounding weaning environment from birth to 28 days postpartum (1,119 samples in total) reared under identical conditions. Source-tracking analysis revealed that the contribution of various microbial sources to the piglet gut microbiome gradually changed over time. The neonatal microbiota was initially sparsely populated and predominantly comprised taxa from the maternal vaginal microbiota that increased gradually from 69.0% at day 0 to 89.3% at day 3 and dropped to 0.28% at day 28. As the piglets aged, the major microbiota community patterns were most strongly associated with the sow feces and slatted floor, with contributions increasing from 0.52% and 9.6% at day 0 to 62.1% and 33.8% at day 28, respectively. The intestinal microbial diversity, composition and function significantly changed as the piglets aged, and 30 age-discriminatory bacterial taxa were identified with distinctive time-dependent shifts in their relative abundance, which likely reflected the effect of the maternal and environmental microbial sources on the selection and adaptation of the piglet gut microbiota.Conclusions: Vaginal microbiota is the primary source of the gut microbiota in piglets within three days after birth and are gradually replaced by the sow fecal and slatted floor microbiota over time. These finding may offer novel strategies to promote the establishment of exogenous symbiotic microbes to improve piglet gut health.
The Effect of Patient Empowerment on Anxiety, Depression or Health-Related Quality of Life in Patients With Type 2 Diabetes: A Systematic Review Protocol
Authors: Duarte-Díaz Andrea , Perestelo-Pérez Lilisbeth , Rivero-Santana Amado , Peñate Wenceslao , Álvarez-Pérez Yolanda , Ramos-García Vanesa , González-Pacheco Himar , Goya-Arteaga Libertad , Bonis-Braun Miriam de , González-Martín Silvia , Ramallo-Fariña Yolanda , Serrano-Aguilar Pedro 
Published: September 2020

Abstract
Background. Type 2 Diabetes Mellitus (T2DM) is one of the most prevalent health problems worldwide. Besides metabolic and cardiovascular complications, nearly one in four patients with T2DM suffer from comorbid depression and it has been reported higher incidence and prevalence of anxiety disorders. Beyond metabolic and cardiovascular risk improvement, empowering patients could contribute to mental health and quality of life enhancement. This Systematic Review (SR) aims to analyze and synthetize the evidence about the effect of patient empowerment on anxiety, depression and health-related quality of life (HRQOL).Methods. A SR of the literature will be conducted according to the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) guidelines. The review will include studies reporting the effect of patient empowerment on anxiety, depression or HRQOL in patients with a T2DM diagnosis. We will use the following databases: Medline, Embase, PsycInfo and Cochrane Library, from inception through July 2020. Additionally, the database searches will be supplemented by searching through citations and references. Literature searches, identification of eligible studies, data extraction, and bias assessment will be undertaken independently by at least two researchers. All disagreements will be resolved by an independent third reviewer. If heterogeneity between studies is too high or it is not possible to conduct a meta-analysis, a narrative analysis of the study results will be provided. Discussion. Existing evidence suggest that empowerment-based strategies significantly improve knowledge, anxiety and depressive levels, self-care, and motivation; contributing to increase HRQOL in patients with T2DM. The results of this SR will provide a deeper understanding on the relationship between patient empowerment and psychosocial outcomes in T2DM.Systematic review registration: PROSPERO CRD42020192429.
Distinct B cell subsets give rise to antigen-specific antibody responses against SARS-CoV-2
Authors: Wilson Patrick , Stamper Christopher , Dugan Haley , Li Lei , Asby Nicholas , Halfmann Peter , Guthmiller Jenna , Zheng Nai-Ying , Huang Min , Stovicek Olivia , Wang Jiaolong , Madariaga Maria Lucia , Shanmugarajah Kumaran , Jansen Maud , Amanat Fatima , Stewart Isabelle , Changrob Siriruk , Utset Henry , Huang Jun , Nelson Christopher , Dai Ya-Nan , Hall Paige , Jedrzejczak Robert , Joachimiak Andrzej , Krammer Florian , Fremont Daved , Kawaoka Yoshihiro 
Published: September 2020

Abstract
Discovery of durable memory B cell (MBC) subsets against neutralizing viral epitopes is critical for determining immune correlates of protection from SARS-CoV-2 infection. Here, we identified functionally distinct SARS-CoV-2-reactive B cell subsets by profiling the repertoire of convalescent COVID-19 patients using a high-throughput B cell sorting and sequencing platform. Utilizing barcoded SARS-CoV-2 antigen baits, we isolated thousands of B cells that segregated into discrete functional subsets specific for the spike, nucleocapsid protein (NP), and open reading frame (ORF) proteins 7a and 8. Spike-specific B cells were enriched in canonical MBC clusters, and monoclonal antibodies (mAbs) from these cells were potently neutralizing. By contrast, B cells specific to ORF8 and NP were enriched in naïve and innate-like clusters, and mAbs against these targets were exclusively non-neutralizing. Finally, we identified that B cell specificity, subset distribution, and affinity maturation were impacted by clinical features such as age, sex, and symptom duration. Together, our data provide a comprehensive tool for evaluating B cell immunity to SARS-CoV-2 infection or vaccination and highlight the complexity of the human B cell response to SARS-CoV-2.
Disruption of the Gut Microbiota Attenuates Epithelial Ovarian Cancer Sensitivity to Cisplatin Therapy
Authors: Chambers Laura , Esakov Emily , Braley Chad , Trestan Lexie , Alali Zahraa , Bazeley Peter , Bayik Defne , Lathia Justin D , Sangwan Naseer , Joehlin-Price Amy S , Dwidar Mohammed , Hajjar Adeline , Ahern Philip P , Claesen Jan , Rose Peter , Vargas Roberto , Michener Chad , Reizes Ofer 
Published: September 2020

Abstract
Background: Epithelial Ovarian Cancer (EOC) is the second most common gynecologic malignancy in the United States, but the leading cause of gynecologic cancer death. Despite many achieving remission with first-line therapy, up to 80% of patients will recur and require additional treatment. Antibiotic therapy is frequently used during cancer treatments for both prophylaxis and treatment of infections, although this profoundly impacts the gut microbiome. Multiple studies suggest that an unperturbed gut microbiome may provide a protective microenvironment, and disruption may be permissive to tumor growth and chemotherapy resistance, including platinum agents. Experimental Design: We assessed whether antibiotic therapy would impact growth of EOC and sensitivity to cisplatin in murine models. Immune competent or compromised mice were given either metronidazole, ampicillin, vancomycin, and neomycin (ABX) containing or control water for two weeks before being intraperitoneally injected with murine ID8 or ID8-VEGF EOC cells. Tumors were monitored and cisplatin therapy was administered weekly until endpoint. Stool was collected throughout the study to asses for microbial population effects over time.Results: Both immune competent and immune compromised ID8 and ID8 VEGF tumor-bearing mice demonstrated a decreased response to cisplatin therapy in ABX treated groups with an increase in overall tumor burden. RNAseq analysis showed enrichment of multiple cell proliferation and stem cell pathways, and stem cell genes SOX2, WNT and PAX2. The self-renewal of ABX treated tumor cells was also increased.Conclusion: Collectively, these studies indicate an intact microbiome provides a tumor suppressive microenvironment and enhances sensitivity to cisplatin.
The Enigma in Management of Complicated Foreign Body Ingestion Impacted Distal to Oesophagus: A Case Series and Literature Review
Authors: Lim Raymond Zhun Ming , Ang Addy Aun Wei , Tan Jih Huei , Lee Ee Peng , Chiew Jun Loong , Tan Henry Chor Lip 
Published: September 2020

Abstract
The authors have requested that this preprint be removed from Research Square.
Implementation of Antimicrobial Stewardship Measures in Cardiac Surgery: Change of Protocol for Surgical Antibiotic Prophylaxis and Its Impact on Surgical Site Infections
Authors: Surat Güzin , Bernsen Dominik , Schimmer Christoph 
Published: September 2020

Abstract
Objectives:The goal of this study was to evaluate the impact on a protocol change of surgical antimicrobial prophylaxis in cardiac surgery comparing the new in hospital prophylactic agent cefazolin versus the former standard antibiotic cefuroxime, initiated by the antimicrobial stewardship team. Methods: This retrospective observational study was performed in a tertiary care hospital in collaboration with the department of cardiothoracic surgery and the antimicrobial stewardship team following a new recommendation of the surgical antimicrobial prophylaxis and included 1029 patients who underwent cardiac surgery. Two patient groups including 582 patients receiving Cefuroxime and 447 patients receiving Cefazolin respectively were compared without altering any other preventative perioperative measures. Adherence, overall infection rates, leg wound infections and deep sternal wound infections were compiled and analysed as well as a broad profile of preoperative, intraoperative and postoperative patient data. Results:A complete adherence on the change of the agent was achieved.Overall surgical site infections occurred in 37 (6.6%) of the cases, with 20 (3,4%) SSI in cefuroxime patients and 17 (3,8%) in cefazolin patients (p-value=0,754). No statistically significant differences could be found in any of the primary and secondary endpoints including DSWI (2,1% for cefuroxime, 1,3% for cefazolin), vein harvesting site infections (1,2% for cefuroxime, 2,2% for cefazolin) as well as urinary tract infections, pneumonia, sepsis and overall mortality of all causes. Conclusions:These results demonstrate that compliance to antimicrobial stewardship standards is not associated with more surgical site infections.
Effects of Red Blood Cell Transfusions on Morbidity and Mortality in Non-Septic Critically Ill Patients; A Propensity Score Matched Study
Authors: Kander Thomas , Nilsson Caroline Ulfsdotter , Larsson Daniel , Bentzer Peter 
Published: September 2020

Abstract
BackgroundRBC-transfusions can be lifesaving, but are also associated with harm. To further examine any effect of red blood cell (RBC)-transfusions given to critically ill patients that were not exposed to the risks of anemia or sepsis, we designed this retrospective propensity score matched study. The aim was to compare mortality and morbidity in non-septic critically ill patients that were given low-grade RBC-transfusions at hemoglobin level > 70 g/L with patients without RBC-transfusions any of the first 5 days in intensive care.MethodsAdult patients admitted to a general 9-bed intensive care unit between 2007-2018 at a tertiary university hospital, were eligible for inclusion. Patients that received > 2 units RBC-transfusion during the first five days after admission, with pre-transfusion hemoglobin level <70 g/L or with severe sepsis or septic shock were excluded. Outcomes were 28-, 90- and 180-day mortality, highest acute kidney injury network (AKIN) score, days alive and free of organ support the first 28 days and highest sequential organ failure assessment score (SOFA-max).ResultsIn total 9491 admissions were recorded during the study period. Propensity score matching at 1:1 ratio resulted in two well matched group with 682 unique patients in each. Median pre-transfusion hemoglobin was 98 g/L (interquartile range 91-106 g/L). Mortality at the measured time points were higher in the RBC-group with an absolute risk increase for death at 180 days of 4.8% [95% confidence interval 2.5 to 7.2%], (p<0.001). Low grade RBC-transfusion was also associated with renal, circulatory and respiratory failure as well as higher SOFA-max score. ConclusionLow-grade leukoreduced RBC-transfusions given to non-septic critically ill patients without significant anemia, was strongly associated with increased mortality, increased kidney-, circulatory- and respiratory- failure as well as with higher SOFA-max score. These findings further strengthen the evidence supporting a restrictive use of RBC-transfusions in critically ill patients.