bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bortezomib consolidation or maintenance following immunochemotherapy and autologous stem cell transplantation for mantle cell lymphoma: CALGB/Alliance 50403
Authors: Kaplan Lawrence D. , Maurer Matthew J. , Stock Wendy , Bartlett Nancy L. , Fulton Noreen , Pettinger Adam , Byrd John C. , Blum Kristie A. , LaCasce Ann S. , Hsi Eric D. , Liu Yi Tian , Scott David W. , Hurd David , Ruppert Amy S. , Hernandez‐Ilizaliturri Francisco , Leonard John P. , Cheson Bruce D. 
Published: March 2020

AbstractImmunochemotherapy followed by autologous transplant (ASCT) in CALGB/Alliance 59909 achieved a median progression‐free survival (PFS) in mantle cell lymphoma (MCL) of 5 years, but late recurrences occurred. We evaluated tolerability and efficacy of adding post‐transplant bortezomib consolidation (BC) or maintenance (BM) to this regimen in CALGB/Alliance 50403, a randomized phase II trial. Following augmented‐dose R‐CHOP/ methotrexate, high‐dose cytarabine‐based stem cell mobilization, cyclophosphamide/carmustine/etoposide (CBV) autotransplant, and rituximab, patients were randomized to BC (1.3 mg/m2 IV days 1, 4, 8, 11 of a 3‐week cycle for four cycles) or BM (1.6 mg/m2 IV once weekly × 4 every 8 weeks for 18 months) beginning day 90. The primary endpoint was PFS, measured from randomization for each arm. Proliferation signature, Ki67, and postinduction minimal residual disease (MRD) in bone marrow were assessed. Of 151 patients enrolled; 118 (80%) underwent ASCT, and 102 (68%) were randomized. Both arms met the primary endpoint, with median PFS significantly greater than 4 years (P < .001). The 8‐year PFS estimates in the BC and BM arms were 54.1% (95% CI 40.9%‐71.5%) and 64.4% (95% 51.8%‐79.0%), respectively. Progression‐free survival was significantly longer for transplanted patients on 50403 compared with those on 59909. Both the PFS and OS were significantly better for those who were MRD‐negative post‐induction. The high risk proliferation signature was associated with adverse outcome. Both BM and BC were efficacious and tolerable, although toxicity was significant. The comparison between studies 50403 and 59909 with long‐term follow up suggests a PFS benefit from the addition of BC or BM post‐ transplant.
An ACVRL1 gene mutation presenting as vein of Galen malformation at prenatal diagnosis
Authors: De Luca Caterina , Bevilacqua Elisa , Badr Dominique A. , Cannie Mieke M. , Sanchez Teresa C. , Segers Valérie , Keymolen Kathelijn , Jani Jacques C. 
Published: March 2020

AbstractHereditary hemorrhagic telangiectasia (HHT) is a rare autosomal dominant disease. The diagnostic criteria of HHT, or Curaçao criteria, include the following: recurrent epistaxis or nighttime nose bleeding, mucocutaneous telangiectases, visceral arteriovenous malformation, or an appropriate family history. The diagnosis is classified as definite if three criteria are present, possible if two criteria are present, and unlikely if only one is present. Nowadays, the confirmation of HHT diagnosis is based on molecular genetic studies. It has been showed that only mutations of genes encoding proteins within the transforming growth factor beta signaling pathway were responsible for the manifestation of the disease. The vein of Galen malformation (VOGM) as a presenting sign of HHT is rare. The prenatal diagnosis of HHT is even rarer. Herein, we present a case of prenatally diagnosed case of HHT based on the presence of VOGM in the fetus. To our knowledge, it is the first time that the gene mutation discovered in this case manifested as VOGM in the fetal life.
Human sex chromosome aneuploidies: The hypothalamic–pituitary–gonadal axis
Authors: Rogol Alan D. 
Published: March 2020

AbstractSex chromosome aneuploidies (SCA) are relatively common as a group, perhaps 1 per 500 births, but much more common at conception. Many syndromes have been noted in those with these conditions, but not so many data are available concerning the hypothalamic–pituitary–gonadal (HPG) axis. The physiology of the HPG axis is first reviewed at four epochs in time: fetal, birth and mini‐puberty, childhood, and adolescence (puberty). Those sections are followed by detailed analysis of the functioning of the HPG axis in individuals with specific SCA with chromosomal numbers ranging from 45 to 49. Robust data are available for the chromosomal complements 47,XXY and 47,XXX with fewer data available for many of the others.
Chemoselective and Site‐Selective Lysine‐Directed Lysine Modification Enables Single‐Site Labeling of Native Proteins
Authors: Adusumalli Srinivasa Rao , Rawale Dattatraya Gautam , Thakur Kalyani , Purushottam Landa , Reddy Neelesh C. , Kalra Neetu , Shukla Sanjeev , Rai Vishal 
Published: March 2020

AbstractThe necessity for precision labeling of proteins emerged during the efforts to understand and regulate their structure and function. It demands selective attachment of tags such as affinity probes, fluorophores, and potent cytotoxins. Here, we report a method that enables single‐site labeling of a high‐frequency Lys residue in the native proteins. At first, the enabling reagent forms stabilized imines with multiple solvent‐accessible Lys residues chemoselectively. These linchpins create the opportunity to regulate the position of a second Lys‐selective electrophile connected by a spacer. Consequently, it enables the irreversible single‐site labeling of a Lys residue independent of its place in the reactivity order. The user‐friendly protocol involves a series of steps to deconvolute and address chemoselectivity, site‐selectivity, and modularity. Also, it delivers ordered immobilization and analytically pure probe‐tagged proteins. Besides, the methodology provides access to antibody‐drug conjugate (ADC), which exhibits highly selective anti‐proliferative activity towards HER‐2 expressing SKBR‐3 breast cancer cells.
Synthesis, Structure, and Bonding of d3 Molybdenum–Oxo Complexes
Authors: Vibbert Hunter B. , Filatov Alexander S. , Hopkins Michael D. 
Published: March 2020

AbstractReduction of d2 metal–oxo ions of the form [MO(PP)2Cl]+ (M=Mo, W; PP=chelating diphosphine) produces d3 MO(PP)2Cl complexes, which include the first isolated examples in group 6. The stability and reactivity of the MO(PP)2Cl compounds are found to depend upon the steric bulk of the phosphine ligands: derivatives with bulky phosphines that shield the oxo ligand are stable enough to be isolated, whereas those with phosphines that leave the oxo ligand exposed are more reactive and observed transiently. Magnetic measurements and DFT calculations on MoO(dppe)2Cl indicate the d3 compounds are low spin with a 2[(dxy)2(π*(MoO))1] configuration. X‐ray crystallographic and vibrational‐spectroscopic studies on d2 and d3 [MoO(dppe)2Cl]0/+ establish that the d3 compound possesses a reduced M−O bond order and significantly longer Mo−O bond, accounting for its greater reactivity. These results indicate that the oxo‐centered reactivity of d3 complexes may be controlled through ligand variation.
Nanoarchitectonics beyond Self‐Assembly: Challenges to Create Bio‐Like Hierarchic Organization
Authors: Ariga Katsuhiko , Jia Xiaofang , Song Jingwen , Hill Jonathan P. , Leong David Tai , Jia Yi , Li Junbai 
Published: March 2020

AbstractIncorporation of non‐equilibrium actions in the sequence of self‐assembly processes would be an effective means to establish bio‐like high functionality hierarchical assemblies. As a novel methodology beyond self‐assembly, nanoarchitectonics, which has as its aim the fabrication of functional materials systems from nanoscopic units through the methodological fusion of nanotechnology with other scientific disciplines including organic synthesis, supramolecular chemistry, microfabrication, and bio‐process, has been applied to this strategy. The application of non‐equilibrium factors to conventional self‐assembly processes is discussed on the basis of examples of directed assembly, Langmuir–Blodgett assembly, and layer‐by‐layer assembly. In particular, examples of the fabrication of hierarchical functional structures using bio‐active components such as proteins or by the combination of bio‐components and two‐dimensional nanomaterials, are described. Methodologies described in this review article highlight possible approaches using the nanoarchitectonics concept beyond self‐assembly for creation of bio‐like higher functionalities and hierarchical structural organization.
Lignocellulosic derivative and chitosan bioadsorbent: Synthesis, characterization, and performance in chromium adsorption
Authors: de Andrade Neto José Carlos , Pereira Gilberto José , de Araújo Morandim‐Giannetti Andreia 
Published: March 2020

AbstractEnvironmental pollution with heavy metals, especially with hexavalent chromium, is a serious problem worldwide. Therefore, in this study, the synthesis, characterization, and application of a heavy metal bioadsorbent, CMCCHDIC, were carried out. Carboxymethylcellulose (CMC) was synthesized from corn stover and reacted with chitosan (CH) usingN,N′‐diisopropylcarbodiimide (DIC) as a coupling reagent to obtain CMCCHDIC. CMC and CMCCHDIC were characterized by granulometric distribution of microparticles, scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, and nuclear magnetic resonance. The ideal conditions for CMCCHDIC adsorption were determined, and 180 min of contact time, pH 2.5, and 293 K were observed to yield the best adsorption capacity. An increase in the adsorption capacity of CMCQDIC (qe= 115.78 ± 0.17 mg∙g−1) to 81.5% relative to that of pure CH (qe= 63.78 ± 0.48 mg∙g−1) was observed, and the reduction of solubility in acidic media comparing with CH, which favors the application of the bioadsorbent synthetized.
Improved mechanical properties of natural rubber composites reinforced by novel SiO2@HCNFs nanofillers at a low filler loading
Authors: Jin Yongzhong , Zheng Xinglong , Wang Lu , Chen Jian , Wang Cheng , Li Binghong 
Published: March 2020

AbstractIn order to improve high reinforcement properties of natural rubber (NR), SiO2@HCNFs as novel double‐phase nanofillers at low content have been loaded in NR by using mechanical mixing method. The morphologies and structures of SiO2@HCNFs and NR composites were characterized, and the performances of NR composites were measured. The results show that compared with pure N330/NR, the modulus at 300% strain, tensile strength, elongation at break of NR composites increase by 10.7, 17.9, and 9.0%, respectively, at only 2.5 phr SiO2@HCNFs content. Meanwhile, the volume abrasion of NR composites is also dramatically reduced at 2.5 phr SiO2@HCNFs content, about 53.4% less than that of N330/NR, though the shore hardness increases by only 3.7%. It is also found that NR composites reinforced by SiO2@HCNFs at 2.5 phr content have much higher hardness and abrasion performance than HCNFs/NR. The DMA results show that high wet skid resistance and low rolling resistance of NR composites were also achieved by loading 6.5 phr SiO2@HCNFs. The unique structure of SiO2@HCNFs double‐phase nanofillers plays a crucial role in properties of NR composites, in virtue of the significant synergetic reinforcing effect of both HCNFs and silica.
One‐step building of molecularly imprinted polymer microcapsules with multicore structure by Pickering emulsion polymerization
Authors: Yan Ruiye , Wang Zehu , Qiu Teng , Li Xiaoyu 
Published: March 2020

AbstractNovel molecularly imprinted polymer (MIP) microcapsules with multicore structure were prepared through one step Pickering emulsion polymerization combined with the polymerization driven phase separation. Bisphenol A (BPA) is found to play the part of heterogeneous nuclei besides template molecule, which offers opportunity for the building of the multicore structure inside of MIP microcapsules. The internal structure of MIP microcapsules could be regulated by choosing different kinds of template molecules. Rebinding experiments showed that the MIP with multicore structure possessed excellent binding properties toward BPA. The imprinting factor of MIP toward BPA is 1.96. The binding could reach equilibrium within 30 min. The fast rebinding kinetics was attributed to the unique multicore structure which reduced largely the mass transfer resistance of BPA in the MIP.
Air filter media functionalized with β‐Cyclodextrin for efficient adsorption of volatile organic compounds
Authors: Kadam Vinod , Kyratzis Ilias L. , Truong Yen B. , Wang Lijing , Padhye Rajiv 
Published: March 2020

AbstractRespiratory air filter media invariably comprise a nonwoven textile fabric. The limitation of the existing fibrous air filter medium is negligible protection against hazardous volatile organic compounds (VOCs). In this study, the filter media was cross‐linked with β‐Cyclodextrin for the surface functionalization. The VOC adsorption performance of the functional fabric was explored for styrene, benzene, and formaldehyde and also compared with a commercial nonwoven face mask. The functionalized fabric performed much better than commercial masks with adsorption capabilities of 18.80 μg mg−1 (for xylene), 14.75 μg mg−1 (for benzene), and 0.06 μg mg−1 (for formaldehyde). Further, the cross‐linking did not affect the air permeability (204 cm3 cm−2 s−1) thus showing no resistance to breathing. β‐Cyclodextrin functionalized textiles can be promising respiratory air filter media having the potential to capture a variety of VOCs yet remain breathable.
Global health crises are also information crises: A call to action
Authors: Xie Bo , He Daqing , Mercer Tim , Wang Youfa , Wu Dan , Fleischmann Kenneth R. , Zhang Yan , Yoder Linda H. , Stephens Keri K. , Mackert Michael , Lee Min Kyung 
Published: March 2020

AbstractIn this opinion paper, we argue that global health crises are also information crises. Using as an example the coronavirus disease 2019 (COVID‐19) epidemic, we (a) examine challenges associated with what we term “global information crises”; (b) recommend changes needed for the field of information science to play a leading role in such crises; and (c) propose actionable items for short‐ and long‐term research, education, and practice in information science.
Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community
Authors: Wen Zhiqiang , Ledesma‐Amaro Rodrigo , Lu Minrui , Jiang Yu , Gao Shuliang , Jin Mingjie , Yang Sheng 
Published: March 2020

AbstractSynthetic microbial communities have become a focus of biotechnological research since they can overcome several of the limitations of single‐specie cultures. A paradigmatic example is Clostridium cellulovorans DSM 743B, which can decompose lignocellulose but cannot produce butanol. Clostridium beijerinckii NCIMB 8052 however, is unable to use lignocellulose but can produce high amounts of butanol from simple sugars. In our previous studies, both organisms were cocultured to produce butanol by consolidated bioprocessing. However, such consolidated bioprocessing implementation strongly depends on pH regulation. Since low pH (pH 4.5–5.5) is required for butanol fermentation, C. cellulovorans cannot grow well and saccharify sufficient lignocellulose to feed both strains at a pH below 6.4. To overcome this bottleneck, this study engineered C. cellulovorans by adaptive laboratory evolution, inactivating cell wall lyases genes (Clocel_0798 and Clocel_2169), and overexpressing agmatine deiminase genes (augA, encoded by Cbei_1922) from C. beijerinckii NCIMB 8052. The generated strain WZQ36: 743B*6.0*3△lyt0798△lyt2169‐(pXY1‐Pthl‐augA) can tolerate a pH of 5.5. Finally, the alcohol aldehyde dehydrogenase gene adhE1 from Clostridium acetobutylicum ATCC 824 was introduced into the strain to enable butanol production at low pH, in coordination with solvent fermentation of C. beijerinckii in consortium. The engineered consortium produced 3.94 g/L butanol without pH control within 83 hr, which is more than 5‐fold of the level achieved by wild consortia under the same conditions. This exploration represents a proof of concept on how to combine metabolic and evolutionary engineering to coordinate coculture of a synthetic microbial community.
Analysis of chemical constituents and six compounds in Qu‐feng‐sheng‐shi Granules via HPLC–ESI–Q/TOF–MSn and HPLC–UV technique
Authors: Yang Pengshuo , Gao Ruilin , Liu Ziyao , Qu Qingsong , Yang Chang , Shi Xinyuan , Lian Zenglin 
Published: March 2020

AbstractQu‐feng‐sheng‐shi Granules (QFSSG), a common prescription for the treatment of chronic inflammation and allergic rhinitis, is widely used in the clinic as a traditional Chinese medicine. Chemical analysis and quality control studies of this formulation are relatively limited compared with pharmacological studies. In this study, a high‐performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry (HPLC–ESI–Q/TOF–MSn) was used to identify the components in this prescription. Next, to quantify six major compounds, an HPLC–UV method was developed and validated. The results showed that 53 compounds were identified based on the MSn data, retention time and previous reports, including 17 coumarins, 14 lignans, 10 chromones, nine phenylethanoid glycosides and three other compounds, were identified or tentatively assigned. Contents of six major bioactive compounds (4′‐O‐β‐glucopyranosyl‐5‐O‐methylvisamminol, Prim‐O‐glucosylcimifugin, forsythin, magnolin, imperatorin, isoimperatorin) could be determined by HPLC simultaneously. In addition, the potential anti‐inflammatory activity of six major compounds was determined too, and we found that four compounds (4′‐O‐β‐glucopyranosyl‐5‐O‐methylvisamminol, Prim‐O‐glucosylcimifugin, forsythin, imperatorin) have a potent nitric oxide inhibitory effect. In conclusion, this work provided comprehensive information on the quality control of QFSSG and evaluated the potential biological activity of the main components in QFSSG, which can contribute to understanding and using it more scientifically.
Neurodegeneration in type 2 diabetes: Alzheimer's as a case study
Authors: Madhusudhanan Jalaja , Suresh Gowthaman , Devanathan Vasudharani 
Published: March 2020

AbstractIntroductionRigorous research in the last few years has shown that in addition to the classical mechanism of neurodegeneration, certain unconventional mechanisms may also lead to neurodegenerative disease. One of them is a widely studied metabolic disorder: type 2 diabetes mellitus (T2DM). We now have a clear understanding of glucose‐mediated neurodegeneration, mostly from studies in Alzheimer's disease (AD) models. AD is recognized to be significantly associated with hyperglycemia, even earning the term “type 3 diabetes.” Here, we review first the pathophysiology of AD, both from the perspective of classical protein accumulation, as well as the newer T2DM‐dependent mechanisms supported by findings from patients with T2DM. Secondly, we review the different pathways through which neurodegeneration is aggravated in hyperglycemic conditions taking AD as a case study. Finally, some of the current advances in AD management as a result of recent research developments in metabolic disorders‐driven neurodegeneration are also discussed.MethodsRelevant literatures found from PubMed search were reviewed.ResultsApart from the known causes of AD, type 2 diabetes opens a new window to the AD pathology in several ways. It is a bidirectional interaction, of which, the molecular and signaling mechanisms are recently studied. This is our attempt to connect all of them to draw a complete mechanistic explanation for the neurodegeneration in T2DM. Refer to Figure 3.ConclusionThe perspective of AD as a classical neurodegenerative disease is changing, and it is now being looked at from a zoomed‐out perspective. The correlation between T2DM and AD is something observed and studied extensively. It is promising to know that there are certain advances in AD management following these studies.
Synthesis of 6‐[4‐(4‐Propoxyphenyl)piperazin‐1‐yl]‐9H‐purine Derivatives as Antimycobacterial and Antifungal Agents: In Vitro Evaluation and In Silico Study
Authors: Nadaf Afra Quasar A. , Najare Mahesh S. , Garbhagudi Manjunatha , Mantur Shivaraj , Sunagar Manjunath G. , Gaonkar Supreet , Joshi Shrinivas , Khazi Imtiyaz Ahmed M. 
Published: March 2020

AbstractA series of novel alkyl substituted purines were synthesized. 6‐[4‐(4‐Propoxyphenyl)piperazin‐1‐yl]‐9H‐purine was used as the key starting material, which was synthesized via a multistep protocol and finally subjected for N‐alkylation with various alkyl halides with an aim to get prospective antimicrobial agents. The structures of the novel compounds were established by substantiating them through spectral techniques like 1H‐NMR, 13C‐NMR, FT‐IR and EI‐MS. They were explored for antitubercular activity against Mycobacterium tuberculosis H37RV. Furthermore, they were checked for their antimicrobial activity concerning bacterial and fungal strains. The title compounds exhibited considerable antimicrobial activity without any significant toxicity. In silico studies depicted their good binding profile against Mycobacterium tuberculosis enoyl reductase (InhA; PDB ID: 4TZK) and Candida albicans dihydrofolate reductase (PDB ID: 1AI9). The title compounds obeyed Lipinski's parameters and have exhibited good drug‐like properties.
A global registry of fractional flow reserve (FFR)–guided management during routine care: Study design, baseline characteristics and outcomes of invasive management
Authors: Schampaert Erick , Kumar Gautam , Achenbach Stephan , Galli Stefano , Tanaka Nobuhiro , Teraphongphom Tarn , Ginn Greg , Zhang Zhen , Somi Samer , Amoroso Giovanni , Brueren Guus , Krasnow Joshua , Knaapen Paul , Berry Colin ,   the PRESSUREwire Investigators
Published: March 2020

AbstractBackgroundThe use and clinical outcomes of fractional flow reserve (FFR)–guided revascularization in patients presenting with either stable coronary artery disease (CAD) or an acute coronary syndrome (ACS) in daily clinical practice are uncertain.ObjectiveTo prospectively characterize the frequency of the change in treatment plan when FFR is performed compared to the initial decision based on angiography alone and procedure‐related outcomes.MethodsWe undertook a prospective, multicenter, multinational, open‐label, observational study of coronary physiologic measurements during clinically indicated coronary angiography. The treatment plan, including medical therapy, PCI or CABG, was prospectively recorded before and after performing FFR. Adverse events were pre‐defined and prospectively recorded per local investigators (PRESSUREwire; ClinicalTrials.gov identifier: NCT02935088).ResultsTwo thousand two hundred and seventeen subjects were enrolled in 70 hospitals across 15 countries between October 2016–February 2018. The mean FFR (all measurements) was 0.84. The treatment plan based on angiography‐alone changed in 763/2196 subjects (34.7%) and 872/2931 lesions (29.8%) post‐FFR. In the per‐patient analysis, the initial treatment plan based on angiography versus the final treatment plan post‐FFR were medical management 1,350 (61.5%) versus 1,470 (66.9%) (p = .0017); PCI 717 (32.7%) versus 604 (27.5%) (p = .0004); CABG 119 (5.4%) versus 121 (5.5%) (p = .8951). The frequency of intended revascularization changed from 38.1 to 33.0% per patient (p = .0005) and from 35.5 to 29.6% per lesion (p < .0001) following FFR.ConclusionsOn an individual patient basis, use of FFR in everyday practice changes the treatment plan compared to angiography in more than one third of all‐comers selected for physiology‐guided managements. FFR measurement is safe, providing incremental information to guide revascularization decisions.