bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative transcriptome analysis of Locusta migratoria tibetensis Chen (Orthoptera: Oedipodidae) under high‐ and low‐temperature stress
Authors: Ran Lu‐yao , Jiang Chun‐xian , Yang Qun‐fang , Wang Hai‐jian , Bai ma Tun‐zhu , Chen Li , Kuang Jian‐kang , Huang Ting‐ting , Li Qing 
Published: January 2020

AbstractThe endemic locust species on the Tibetan plateau, Locusta migratoria tibetensis Chen can grow and breed successfully under the plateau temperature environment. This study explored the molecular response process of Tibetan locusts to high and low temperatures by comparing its transcriptome under different temperature conditions. A total of 66,256 unigenes were obtained through RNA‐Seq, among which 30,051 were successfully annotated. In detail, there were 778 differentially expressed genes (DEGs) at high temperatures and 1,787 DEGs at low temperatures. Under high temperatures, the metabolic pathways of saturated and unsaturated fatty acids and amino acids were up‐regulated, producing a significant amount of fatty acids and amino acids. However, under low temperatures, the chitinase activity was enhanced, causing chitin to decompose into small sugar molecules. Meanwhile, various sugar metabolism pathways were up‐regulated when a significant amount of small sugar molecules were produced. Then, large quantities of alpha‐linolenic acid and arachidonic acid were accumulated to maintain the normal physiological functions of cells. The analysis of annotated genes revealed that heat shock proteins (Hsp) were associated with the response to temperature stress, and mainly involved in the synthesis and transportation of proteins, preventing protein damage and the decomposition of damaged proteins. These results provide a basis for the further understanding of the adaptation mechanism of Tibetan locusts to high and low temperatures, laying a foundation for further understanding the adaptability of Tibetan locusts on Tibetan plateaus.
Influence of cecotrophy on fat metabolism mediated by caecal microorganisms in New Zealand white rabbits
Authors: Li Ruiting , Li Xiuling , Huang Tao , Wang Yadong , Xue Mingming , Sun Shuaijie , Yan Duo , Song Guohua , Sun Guirong , Li Ming 
Published: January 2020

AbstractCecotrophy is a special behaviour of rabbits. Eating soft faeces can improve feed efficiency and maintain gut flora in rabbits. In our previous study, we found that fasting from soft faeces significantly reduced growth rate and total cholesterol (TC) in New Zealand white rabbits (NZW rabbits), thereby resulting in lower values for body weight and fat deposition in the soft faeces fasting group than in the control group. However, it has not been demonstrated whether cecotrophy by NZW rabbits can regulate lipid metabolism by changing the diversity of caecal microorganisms. In this study, thirty‐six 28‐day‐old weaned NZW female rabbits were randomly divided into two groups (the soft faeces fasting group and the control group) and fed to 90 days. Rabbits in the experimental group were treated with an Elizabeth circle to prevent them from eating their soft faeces. Then, the caecal contents of three rabbits from the soft faeces fasting group and three rabbits from the control group were collected for metagenomic sequencing. We found that the abundance of Bacteroides increased, while Ruminococcus decreased, compared with the control group after fasting from soft faeces. Relative abundance was depressed for genes related to metabolic pathways such as ascorbate and aldarate metabolism, riboflavin metabolism and bile secretion. Moreover, there was a general correlation between variation in microbial diversity and fat deposition. Bacteroides affects body weight and TC by participating in the riboflavin metabolism pathway. By investigating the effect of cecotrophy on caecal microorganisms of rabbits, we identified the key microorganisms that regulate the rapid growth performance of NZW rabbits, which may provide useful reference for the future research and development of microecological preparations for NZW rabbits.
Ecological health assessment of the Ousteri wetland in India through synthesizing remote sensing and inventory data
Authors: Irfan Zareena Begum , Ling Venkatachalam , Shan Jayakumar 
Published: January 2020

AbstractOusteri Lake is one of the important wetlands in India, harbouring many different types of flora and providing a suitable habitat for many different fauna, being declared as a sanctuary in October 2008. The National Wetland Conservation Program of Ministry of Environment and Forests identified Ousteri Lake as a nationally important wetland, and the Bombay Natural History Society (BNHS) named it an Important Bird Area (IBA). The International Union for Conservation of Nature and Natural Resources (IUCN) also has identified this lake as a heritage site. The present study examined the state of environment in and around the Ousteri wetland, assessing the probable threats to the lake and its ecological environs. Ousteri Lake supports diverse rich and rare flora and fauna. Because of its extreme hydrological fluctuations over the annual cycle, the lake exhibits interesting patterns of flora and fauna. The north‐east monsoons leave the lake flooded during the winter months, while the scorching summers leave it totally dry. Ousteri Lake had been primarily used as a tank for irrigation purposes, with ~2,000 acres being cultivated. The main crop is paddy, and the only subsidiary is sugar cane. The natural vegetation, significantly altered by human activities, consists of over 220 species belonging to 63 families. The vegetation study conducted earlier have reported a total of 472 plant species, with the herbs being 40% of this total, followed by trees (21%). Other life forms in the lake are represented by less than 100 species. Ousteri Lake and its environs also provide a wide variety of habitat for many floral species, with 190 species being listed as least concern, 23 species as near threatened and 24 species as vulnerable, according to the IUCN Red List. About 14 floral species are being categorized as endemic. Further, about 202 fauna are listed as least concern, 13 species as near threatened, two species as vulnerable, three species as endangered and one as critically endangered. Moreover, five species are in an endemic category.
Tracking pollution and its sources in the catchment‐lake system of major waterbodies in Kazakhstan
Authors: Krupa Elena , Barinova Sophia , Aubakirova Moldir 
Published: January 2020

AbstractThe objective of the present study was to identify the sources and routes of entry of heavy metals and nutrients into several large waterbodies in the arid zone of Kazakhstan, namely Lake Balkhash and Samarkan and Shardara reservoirs. A data visualization method was used for this purpose. Statistical mapping, based on referencing coordinates of the material selection points, was performed with the Statistics 12.0 program. Matching statistical maps with the waterbody contours was performed in Adobe Photoshop. Maps of the spatial distribution of heavy metals (cadmium, nickel, cobalt, lead, zinc and copper) and nutrients were constructed for all three study waterbodies. Data visualization clearly illustrated the relationship between the distribution of pollutants in the study waterbodies, as well as the sources of anthropogenic pollution. Based on the mapping exercise, it was established that Cu and Zn entered the waterbodies mainly in agricultural waste waters, while Cd, Ni, Co, Pb and partly Zn entered the waterbodies mainly in industrial and surface run‐off. Nutrients enter the waterbodies mainly in river and municipal waters. The constructed maps demonstrate the role of the constant flow in the distribution of pollutants in the waters of Lake Balkhash. The results also indicate that statistical mapping is an effective tool for identifying the sources and routes of pollutant entry into waterbodies. The imposition of statistical maps on the contours of waterbodies of concern provides a mapping method allowing for more visually compelling data and information for a wide circle of users and managers.
The effect of a combined intervention on exclusive breastfeeding in primiparas: A randomised controlled trial
Authors: Puharić Drita , Malički Mario , Borovac Josip Anđelo , Šparac Vladimir , Poljak Boris , Aračić Nađa , Marinović Nero , Luetić Nives , Zakarija‐Grković Irena 
Published: January 2020

AbstractAn antenatal/postnatal intervention involving proactive telephone support and written materials was conducted among primiparas. Four hundred women, from the Split‐Dalmatia County, Croatia, were randomized between November 2013 and December 2016 into three groups: intervention (IG), active control (ACG) and standard care (SCG). Primary outcome was exclusive breastfeeding (EBF) at 3 months. Secondary outcomes included breastfeeding difficulties, attitudes towards infant feeding, breastfeeding self‐efficacy and social support. Practice staff were blinded to group allocation. Of 400 women, 45 (11%) were lost to follow‐up, and final analyses were conducted on 129 (IG), 103 (ACG) and 123 (SCG) participants. EBF rates at 3 months were significantly higher for the IG (odds ratio [OR] 4.6, 95% confidence interval [CI], 2.7 to 8.1; EBF 81%) as well as at 6 months (OR 15.7, 95% CI, 9.1 to 27.1; EBF 64%) compared with SCG (EBF 47% at 3 months and 3% at 6 months). Higher rates were also observed for the ACG at 3 months (OR 2.2, 95% CI, 1.3 to 3.8, EBF 68%) and 6 months (OR 2.3, 95% CI, 1.4 to 3.9, EBF 16%). Participants in the IG had the highest increase in positive attitudes towards infant feeding, in comparison to baseline, and significantly higher breastfeeding self‐efficacy. Participants in SCG experienced significantly more breastfeeding difficulties, both at 3 and 6 months, in comparison to AC and IGs. Written breastfeeding materials and proactive telephone support among primiparas are an effective means of increasing breastfeeding rates, decreasing breastfeeding difficulties and improving self‐efficacy and attitudes towards infant feeding.
Health problems among breast cancer survivors after completing conventional treatments: A cross‐sectional study
Authors: Chumdaeng Sureeporn , Soivong Pratum , Sethabouppha Hunsa , Chontawan Ratanawadee 
Published: January 2020

AbstractThis cross‐sectional design study aimed to explore health problems among breast cancer survivors after completing conventional treatments. Purposive sampling was adopted to recruit 160 breast cancer survivors from a regional tertiary care hospital and hospital with a specialist cancer center in southern Thailand. STROBE checklist of items for cross‐sectional studies is applied to report the study. Five most frequent, severe, and distressing symptoms faced in the first 3 years after cancer treatment were reported as numbness to the affected side, difficulty sleeping, fatigue, pain, and skin changes. Almost half of the participants reported symptoms indicating a definite diagnosis of anxiety, and nearly 70% of them rated themselves as borderline depressed or depressed. Approximately 50% of participants reported no change in their relationships with significant people, and about 60% of them reported a high‐level fear of recurrence. Symptom frequency, severity, and distress were positively associated with anxiety, depression, and menopausal symptoms, while relationship changes were negatively associated with menopausal symptoms, anxiety, and depression. Support and information for these women are required after active treatment has ceased to deal with ongoing symptoms.
Molecular oxygen as a signaling component in plant development
Authors: Weits Daan A. , van Dongen Joost T. , Licausi Francesco 
Published: January 2020

SummaryWhile traditionally hypoxia has been studied as a detrimental component of flooding stress, the last decade has flourished with studies reporting the involvement of molecular oxygen availability in plant developmental processes. Moreover, proliferating and undifferentiated cells from different plant tissues were found to reside in endogenously generated hypoxic niches. Thus, stress‐associated acute hypoxia may be distinguished from constitutively generated chronic hypoxia. The Cys/Arg branch of the N‐degron pathway assumes a central role in integrating oxygen levels resulting in proteolysis of transcriptional regulators that control different aspects of plant growth and development. As a target of this pathway, group VII of the Ethylene Response Factor (ERF‐VII) family has emerged as a hub for the integration of oxygen dynamics in root development and during seedling establishment. Additionally, vegetative shoot meristem activity and reproductive transition were recently associated with oxygen availability via two novel substrates of the N‐degron pathways: VERNALISATION 2 (VRN2) and LITTLE ZIPPER 2 (ZPR2). Together, these observations support roles for molecular oxygen as a signalling molecule in plant development, as well as in essential metabolic reactions. Here, we review recent findings regarding oxygen‐regulated development, and discuss outstanding questions that spring from these discoveries.
A new look at the cognitive neuroscience of video game play
Authors: Dale Gillian , Joessel Augustin , Bavelier Daphne , Green C. Shawn 
Published: January 2020

AbstractA growing body of literature has investigated the effects of playing video games on brain function and behavior. One key takeaway from this literature has been that not all entertainment video games are created equal with respect to their effects on cognitive functioning. The majority of the research to date has contrasted the cognitive impact of playing first‐ or third‐person shooter games (together dubbed “action video games”) against the effects of playing other game types. Indeed, when the research began in the late 1990s, action video games placed a load upon the perceptual, attentional, and cognitive systems in a manner not seen in other video games. However, the video game industry has shifted dramatically over the intervening years. In particular, first‐ and third‐person shooter games are no longer unique in the extent to which they load upon cognitive abilities. Instead, a host of other game genres appear to place similar degrees of load upon these systems. This state of affairs calls for a paradigm shift in the way that the cognitive neuroscience field examines the impact of video game play on cognitive skills and their neural mediators—a shift that is only just now slowly occurring.
Integrating naloxone education into an undergraduate nursing course: Developing partnerships with a local department of health
Authors: Carter Gregory , Caudill Penny 
Published: January 2020

AbstractBackgroundSubstance misuse continues to be a significant concern in the United States, with over 700,000 lives lost from a drug overdose between 1999 and 2017. However, nursing curricula have failed to keep pace with the epidemic.MethodsThe current study used a pre‐post study design and involved a convenience sample of undergraduate nursing students (n = 37) enrolled in a community health nursing course. Students completed an 11‐item online survey examining naloxone stigma, naloxone self‐efficacy, and naloxone knowledge. A paired sample t test was conducted to evaluate the impact of the in‐person training and education event.ResultsThere was a statistically significant increase in post‐intervention naloxone knowledge scores from the pre‐survey (M = 3.57, SD = 0.959) to the post‐survey (M = 4.70, SD = 0.520). Stigma toward naloxone demonstrated a statistically significant increase in the post‐intervention stigma survey (M = 9.00, SD = 1.312) compared with the pre‐intervention stigma survey (M = 7.78, SD = 2.228). Naloxone self‐efficacy also demonstrated a statistically significant increase in the post‐intervention naloxone efficacy survey (M = 10.08, SD = 1.064) compared with the pre‐intervention naloxone efficacy scores (M = 7.38, SD = 2.22).ConclusionsThe students’ scores demonstrated a significant increase in naloxone knowledge, self‐efficacy, and stigma. Future research is needed to explore the impact of integrating naloxone education in the undergraduate nursing curriculum.
A broad diversity survey of Rhizoctonia species from the Brazilian Amazon reveals the prevalence of R. solani AG‐1 IA on signal grass and the new record of AG‐1 IF on cowpea and soybeans
Authors: Chavarro‐Mesa Edisson , Ceresini Paulo , Pereira Danilo , Vicentini Samara , Silva Tatiane , Ramos‐Molina Lina , Negrisoli Matheus , Schurt Daniel , Vieira Júnior José R. 
Published: January 2020

AbstractLeaf blight, sheath blight, and web blight are major diseases caused by Rhizoctonia species on both Fabaceae and Poaceae plant hosts in the Brazilian Amazon agroecosystem. To determine the diversity of Rhizoctonia species associated with foliar diseases on fabaceous (cowpea and soybean) and poaceous (rice and signal grass [Urochloa brizantha]) hosts, a broad survey was conducted in Pará, Rondônia, Roraima, and Mato Grosso, in the Amazon, from 2012 to 2013. We extended our survey to Cerrado areas of Mato Grosso, and the lowlands of Paraíba Valley, in São Paulo, where these Rhizoctonia foliar diseases have not been reported so far. Our findings revealed that these diseases are caused by a diversity of Rhizoctonia solani AG‐1 complex. We detected that R. solani AG‐1 IA (sexual phase Thanatephorus cucumeris) was the predominant pathogen associated with signal grass leaf blight and collar rot diseases in the Amazon, especially in Rondônia and northern Mato Grosso. In addition, a subgroup of R. solani (AG‐1 IF), not previously reported in Brazil, was associated with leaf blight on cowpea and soybean, in Roraima. Another subgroup (AG‐1 ID) was also detected in Roraima. In Mato Grosso Cerrados we did not find any of the major Rhizoctonia foliar pathogens. Instead, R. oryzae (Waitea circinata) was the predominant species associated with a collar rot on U. brizantha. In the lowlands of São Paulo, R. oryzae‐sativae (Ceratobasidium oryzae‐sativae) was the predominant pathogen detected causing the rice sheath spot disease.
Inside Lobbying on the Regulation of New Plant Breeding Techniques in the European Union: Determinants of Venue Choices
Authors: Hartung Ulrich 
Published: January 2020

AbstractIn July 2018, the Court of Justice of the European Union decided that new plant breeding techniques (NPBTs) fall within the scope of the restrictive provisions on genetically modified organisms (GMOs). Previously, various actors had lobbied in order to influence the European Union’s (EU’s) regulatory decision on NPBTs. This study examines the venue choices taken by Cibus, a biotech company that promoted NPBT deregulation. It shows that the firm bypassed the EU level and that it lobbied competent authorities (CAs) in certain member states to gain support for the deregulation of NPBTs. Cibus chose the CAs because their institutional “closedness” reduced the risk of the debate over the deregulation of NPBTs becoming public. However, the CA’s specific competences and their influence on EU decision making were of likewise importance. The firm lobbied CAs based in Finland, Germany, Ireland, Sweden, Spain, and the United Kingdom. Two factors appear to have influenced Cibus’ choices for these countries: high‐level political support for agribiotech and the high relevance of biotech sectors. In contrast, public support for GMOs turned out to have hardly any influence, and virtually no association could be observed for the agricultural application of biotechnology in the past nor for the weakness of domestic anti‐GMO lobby groups. Finally, the in‐depth study on Germany affirms that “closedness” was important for Cibus’ choices and reveals that technical information served as a venue‐internal factor that influenced the firm’s choices.
A historical perspective of how public policy shaped dialysis care delivery in the United States
Authors: Anumudu Samaya J. , Eknoyan Garabed 
Published: January 2020

AbstractBroadly defined public policy has been said to be whatever “governments choose to do or not to do” As applied to healthcare, public policy can be traced back to the 4000‐year‐old Code of Hammurabi. As it applies to dialysis care its history is barely 50 years old since national coverage for end‐stage renal disease (ESRD) was legislated as Public Law 92‐603 in 1972. As with most healthcare policy changes, it was a result of medical progress which had changed renal function replacement by dialysis from its rudimentary beginnings during the Second World War into an experimental acute life‐saving procedure in the 1950s and to an established life‐sustaining treatment for the otherwise fatal disease of uremia in the 1960s that was limited by its costs. Since 1973, the Medicare ESRD Program has saved the lives of thousands of individuals, a compassionate achievement that has come at increasing costs which have exceeded all estimates and evaded containment. Apart from cost containment, policy changes in dialysis care have been directed at improving its safety and adequacy. Some of the results of these changes are evident as one compares the outcomes and complications of dialysis encountered in the 1970s to those in the present; others, particularly those related to vascular access and hospitalization rates have improved modestly. This article recounts the historical background in which national coverage for dialysis care was developed, legislated and has evolved over the past 50 years.
Pushing boundaries and blurring categories in digital media and religion research
Authors: Peterson Kristin M. 
Published: January 2020

AbstractAs religious identity and spiritual practices transform and expand in the digital media moment, this article advocates for more critical scholarship on media and religion that examines the complex ways that individuals make meaning in the digital age. First, I present an overview of foundational media and religion theories that analyze the interactions between these ever‐changing fields, such as the culturalist tradition, mediatization theory, and the social shaping of technology approach. Furthermore, this essay highlights insightful research trends that blur distinctions between media spaces and complicate definitions of religion. Finally, a discussion of gaps in the scholarship will justify an argument for more theories centered in international contexts, as well as analysis of the relationships between media technologies, aesthetics, affect, identity and religious expression. These emerging approaches provide more in‐depth discussions of how the fast‐changing and ever‐complex digital culture is deeply connected to the evolving nature of religion and human existence.