bluuebunny/crossref_metadata_2025_split huggingface dataset:
Induction of protection in mice against a respiratory challenge by a vaccine formulated with exosomes isolated from Chlamydia muridarum infected cells
Authors: Pal Sukumar , Mirzakhanyan Yeva , Gershon Paul , Tifrea Delia F. , de la Maza Luis M. 
Published: September 2020

AbstractThe goal of this study was to determine if exosomes, isolated from Chlamydia muridarum infected HeLa cells (C. muridarum-exosomes), induce protective immune responses in mice following vaccination using CpG plus Montanide as adjuvants. Exosomes, collected from uninfected HeLa cells and PBS, formulated with the same adjuvants, were used as negative controls. Mass spectrometry analyses identified 113 C. muridarum proteins in the C. muridarum-exosome preparation including the major outer membrane protein and the polymorphic membrane proteins. Vaccination with C. muridarum-exosomes elicited robust humoral and cell-mediated immune responses to C. muridarum elementary bodies. Following vaccination, mice were challenged intranasally with C. muridarum. Compared to the negative controls, mice immunized with C. muridarum-exosomes were significantly protected as measured by changes in body weight, lungs’ weight, and number of inclusion forming units recovered from lungs. This is the first report, of a vaccine formulated with Chlamydia exosomes, shown to elicit protection against a challenge.
EMILIN proteins are novel extracellular constituents of the dentin-pulp complex
Authors: Imhof Thomas , Korkmaz Yüksel , Koch Manuel , Sengle Gerhard , Schiavinato Alvise 
Published: September 2020

AbstractOdontoblasts and pulp stroma cells are embedded within supramolecular networks of extracellular matrix (ECM). Fibrillin microfibrils and associated proteins are crucial constituents of these networks, serving as contextual scaffolds to regulate tissue development and homeostasis by providing both structural and mechanical properties and sequestering growth factors of the TGF-β superfamily. EMILIN-1, -2, and -3 are microfibril-associated glycoproteins known to modulate cell behaviour, growth factor activity, and ECM assembly. So far their expression in the various cells of the dentin-pulp complex during development, in the adult stage, and during inflammation has not been investigated. Confocal immunofluorescence microscopy and western blot analysis of developing and adult mouse molars and incisors revealed an abundant presence of EMILINs in the entire dental papilla, at early developmental stages. Later in development the signal intensity for EMILIN-3 decreases, while EMILIN-1 and -2 staining appears to increase in the pre-dentin and in the ECM surrounding odontoblasts. Our data also demonstrate new specific interactions of EMILINs with fibulins in the dentin enamel junction. Interestingly, in dentin caries lesions the signal for EMILIN-3 was significantly increased in inflamed odontoblasts. Overall our findings point for the first time to a role of EMILINs in dentinogenesis, pulp biology, and inflammation.
Correlating Neuroimaging and CNVs Data: 7 Years of Cytogenomic Microarray Analysis on Patients Affected by Neurodevelopmental Disorders
Authors: Milone Roberta , Cesario Claudia , Bernardini Laura , Battini Roberta , Goldoni Marina , Pasquariello Rosa , Fusilli Caterina , Giovannetti Agnese , Giglio Sabrina , Novelli Antonio , Caputo Viviana , Cioni Giovanni , Mazza Tommaso , Battaglia Agatino 
Published: September 2020

AbstractThe aim of this study was to evaluate the relationship between neurodevelopmental disorders, brain anomalies, and copy number variations (CNVs) and to estimate the diagnostic potential of cytogenomical microarray analysis (CMA) in individuals neuroradiologically characterized with intellectual developmental disorders (IDDs) isolated or associated with autism spectrum disorders (ASDs) and epilepsy (EPI), all of which were identified as a “synaptopathies.” We selected patients who received CMA and brain magnetic resonance imaging (MRI) over a 7-year period. We divided them into four subgroups: IDD, IDD + ASD, IDD + EPI, and IDD + ASD + EPI. The diagnostic threshold of CMA was 16%. The lowest detection rate for both CMA and brain anomalies was found in IDD + ASD, while MRI was significantly higher in IDD and IDD + EPI subgroups. CMA detection rate was significantly higher in patients with brain anomalies, so CMA may be even more appropriate in patients with pathological MRI, increasing the diagnostic value of the test. Conversely, positive CMA in IDD patients should require an MRI assessment, which is more often associated with brain anomalies. Posterior fossa anomalies, both isolated and associated with other brain anomalies, showed a significantly higher rate of CMA positive results and of pathogenic CNVs. In the next-generation sequencing era, our study confirms once again the relevant diagnostic output of CMA in patients with IDD, either isolated or associated with other comorbidities. Since more than half of the patients presented brain anomalies in this study, we propose that neuroimaging should be performed in such cases, particularly in the presence of genomic imbalances.
Pediatric to Adult Epilepsy Transition in Ambulatory Care: Benefits of a Multidisciplinary Epilepsy Transition Clinic
Authors: Twanow Jaime-Dawn E. , Maturu Sarita , Khandker Nabil 
Published: September 2020

AbstractChildren with epilepsy comprise 3.2% of the estimated 500,000 youth with special medical needs who move from the pediatric to adult care model annually. These 16,000 children who require transfer each year represent a challenging subset of 470,000 youth living with epilepsy in the United States. Transition and transfer of care are complex and require gradual processes. This period for youth with epilepsy is often associated with inadequate follow-up and increased risk of nonadherence. Furthermore, youth and adults with epilepsy are known to have suboptimal social and emotional outcomes compared with peers, with high rates of under education, underemployment, poverty, and struggles with mental health. The goal of improving social determinants and continuity of care prompted the development of formal epilepsy transition clinics. Multiple clinic models exist, sharing the overarching goal of supporting youth while building self-management skills, tailored to age and developmental level. Early evidence shows that transition discussion leads to statistically significant increases in transfer readiness and self-efficacy in young adults with epilepsy. Our center boasts a 100% attendance rate at our transition and transfer clinic and 78% compliance with follow-up, further demonstrating that patients and families value quality transition programming.
The Importance of Assessing and Treating Mental Health in Transition-Aged Adolescents with Epilepsy: A 1-Year Follow-up
Authors: Healy Sarah A. , Fantaneanu Tadeu A. , Whiting Sharon 
Published: September 2020

AbstractMental health issues become especially problematic when adolescents with epilepsy are preparing to transition from pediatric to adult care. Consistent with guidelines, a transition clinic with ongoing mental health assessment was created, providing treatment to patients scoring in the moderate severity range or higher. In order to examine the effectiveness of our epilepsy transition clinic and the impact of mental health in transition-aged adolescents, baseline and one-year follow-up data were compared in 36 participants (M = 15.82 years, 24 males). Results showed that the majority of participants had improved or comparable mental health scores at follow-up. Furthermore, participants who met threshold for mental health treatment had significantly improved mental health (t = 3.19, p = 0.015), while those who did not showed worsened mental health (t =  − 2.50, p = 0.019). Looking specifically at mental health impact, those with worsened mental health showed significantly worsened quality of life (t = 3.35, p = 0.012). Furthermore, those without mental health issues showed improved transition skills (t =  − 3.86, p = 0.002), while those with mental health issues did not. Results suggest that the transition clinic is effective in helping transition-aged adolescents with their mental health. Additionally, findings suggest that addressing these mental health issues are essential to ensuring successful transitions and the best outcomes in these patients.
Sedation and Analgesia Practices in Pediatric Intensive Care Units: A Survey of 27 Centers from Turkey
Authors: Ekinci Faruk , Yildizdas Dincer , Horoz Ozden Ozgur , Aslan Nagehan ,   The Society of Pediatric Emergency Intensive Care Medicine of Turkey-Sedoanalgesia Study Group
Published: September 2020

AbstractThe management and monitoring of sedoanalgesia are important measures in improving the efficacy of procedures and mechanical ventilation, as well as reducing adverse effects and preventing withdrawal syndrome, and delirium in pediatric intensive care units (PICUs). As there is an ongoing need to clarify the best approach to sedoanalgesia in PICUs, we aimed to analyze the current approaches in sedation, analgesia, withdrawal, and delirium practices among PICUs in Turkey. Twenty-seven PICUs completed the survey. Only 9 (33.3%) and 13 (48.1%) centers had a written protocol for analgesia and sedation, respectively. Paracetamol and a combination of midazolam and fentanyl were preferred in 51.8 and 40% of the PICUs for postoperative periods, respectively, and 81.4% of the units preferred ketamine for short-term interventions. For prolonged sedation in mechanically ventilated children, a combination of benzodiazepines and opiates were the most preferred first-line agents with a very high percentage of 81.4%, whereas ketamine and dexmedetomidine accounted for 62.9 and 18.5%, respectively, as second-line options. Although sedative and analgesic agent preferences were comparable with the relevant literature, we should focus on developing a standardized, evidence-based algorithm for sedation and analgesic drugs.
The anti-tumor effect of ursolic acid on papillary thyroid carcinoma via suppressing Fibronectin-1
Authors: Cao Mingxiang , Xiao Di , Ding Xubei 
Published: September 2020

Abstract
This study aims to discover the effects of ursolic acid (UA) on papillary thyroid carcinoma (PTC). Human PTC cells were under UA treatment, and cell viability, clone formation, and apoptosis were measured by MTT assay, clone formation assay, and flow cytometry, respectively. Expressions of apoptosis- and epithelial–mesenchymal transition (EMT)-related markers were determined via qRT-PCR and western blot. Fibronectin-1 (FN1) expression in thyroid carcinoma was analyzed by GEPIA2 and qRT-PCR. The effects of overexpressed FN1 on UA-treated cells were detected following the previous procedures. Cell viability, proliferation, and EMT-related marker expressions were inhibited, while cell apoptosis and apoptosis-related marker expressions were promoted by UA. FN1 was higher expressed in thyroid carcinoma and downregulated by UA. Effects of FN1 on cell viability, proliferation, and apoptosis- and EMT-related marker expressions were partially reversed by UA. UA inhibited human PTC cell viability, proliferation, and EMT but promoted apoptosis via suppressing FN1.
Nickel ferrite doped lithium substituted zinc and niobo vanadate glasses: thermal, physical, and electrical characterization
Authors: Getachew Belay , Ramesh K P , Honnavar Gajanan V 
Published: September 2020

Abstract
This study reports a comparison of some thermal, physical, and electrical properties of lithium substituted zinc and niobo vanadate glasses doped with nickel ferrite prepared for the electrical application. These glasses were prepared using the melt quenching technique. Density and differential scanning calorimetry were used to derive various physical and thermal properties. Packing density, the concentration of atoms, separations between atoms were compared between doped and undoped glass families. Thermal stability was estimated and compared. It is found that glasses with 20 mol% of Lithium are more stable than the rest. The thermal stability increases significantly by doping Nickel Ferrite from 5 °C to 30 °C in zinc vanadate glasses. Packing density varies with mol% of lithium ions from 0.43 to 0.42 in zinc vanadate glasses and from 0.40 to 0.42 in niobo vanadate glasses. Doping of Nickel ferrite varies the packing density from 0.42 to 0.43 in zinc vanadate glasses whereas it stabilizes the packing density to 0.42 in niobo vanadate glasses. Out of the two families of glasses, zinc vanadate glass doped with nickel ferrite shows better stability and higher packing density compared with others. DC electrical conductivities of the two families of glasses are almost similar and nickel ferrite doping has a little effect on DC electrical conductivity. Thus it is concluded that the nickel ferrite enhances the stability of the glass while maintaining DC electrical conductivity. This observation is important from the point of view of the electrical application of these glasses.
Effectiveness and safety of oral anticoagulants among non-valvular atrial fibrillation patients with polypharmacy
Authors: Lip Gregory Y H , Keshishian Allison , Kang Amiee , Dhamane Amol D , Luo Xuemei , Klem Christian , Rosenblatt Lisa , Mardekian Jack , Jiang Jenny , Yuce Huseyin , Deitelzweig Steven 
Published: September 2020

AbstractAimsPolypharmacy is prevalent among non-valvular atrial fibrillation (NVAF) patients and presents a potential issue for the effective management of NVAF. This study compared the risk of stroke/systemic embolism (SE) and major bleeding (MB) among NVAF patients with polypharmacy newly prescribed oral anticoagulants (OACs).Methods and resultsA retrospective study of NVAF patients with polypharmacy who initiated OACs from 01 January 2013 to 30 September 2015 was conducted using US CMS Medicare and four commercial databases. Polypharmacy was defined as ≥6 concomitant medications on the index date. Propensity score matching was conducted to compare non-vitamin K antagonists OACs (NOACs) to warfarin as well as between NOACs. Cox proportional hazard models were used to evaluate the risk of stroke/SE and MB. A total of 188 893 patients with polypharmacy were included, with an average of 8 concomitant medications (interquartile range 6–9). Compared to warfarin, apixaban [hazard ratio (HR): 0.59, 95% confidence interval (CI): 0.52–0.68], and rivaroxaban (HR: 0.75, 95% CI: 0.69–0.83) were associated with a lower risk of stroke/SE. Apixaban (HR: 0.57, 95% CI: 0.54–0.61) and dabigatran (HR: 0.76, 95% CI: 0.66–0.88) were associated with a decreased risk of MB compared with warfarin. Compared with dabigatran and rivaroxaban, apixaban was associated with a lower risk of stroke/SE and MB. Dabigatran was associated with lower risk of MB compared with rivaroxaban.ConclusionsIn this observational study of anticoagulated NVAF patients with polypharmacy, effectiveness and safety profiles are more favourable for NOACs vs. warfarin. Our observations are hypothesis generating and may help inform future clinical trials regarding appropriate OAC treatment selection in polypharmacy patients.
Galois action on homology of generalized Fermat Curves
Authors: Kontogeorgis Aristides , Paramantzoglou Panagiotis 
Published: September 2020

Abstract
The fundamental group of Fermat and generalized Fermat curves is computed. These curves are Galois ramified covers of the projective line with abelian Galois groups H. We provide a unified study of the action of both cover Galois group H and the absolute Galois group $\mathrm{Gal}(\bar{\mathbb{Q}}/\mathbb{Q})$ on the pro-$\ell$ homology of the curves in study. Also the relation to the pro-$\ell$ Burau representation is investigated.
Molecular Diagnosis of Coronavirus Disease 2019
Authors: dos Santos Claudia C. , Zehnbauer Barbara A. , Trahtemberg Uriel , Marshall John 
Published: September 2020

Objectives:
To review molecular diagnostics for coronavirus disease 2019. The world is in the midst of a coronavirus disease 2019 pandemic. Containing the spread of the severe acute respiratory distress coronavirus is critical. Instrumental to the future success is the ability to reliably and reproducibly detect this inciting pathogen to inform public health containment policies and treatment decisions.

Data Sources:
Molecular diagnostics focusing on molecular detection methodologies for detection of the virus and the presence of the disease.

Study Selection:
Narrative review.

Data Extraction:
Literature, PubMed, Scopus, and official government documents.

Data Synthesis:
Diagnosing severe acute respiratory syndrome coronavirus is done through real-time reverse transcriptase-polymerase chain reaction tests, cell culture, and serology. For patients, diagnostics are an integral part of a full medical history, physical examinations, blood tests, and diagnostic imaging.

Conclusions:
Here, we review current approaches to the molecular diagnosis of coronavirus disease 2019.
Oral Antiplatelet Therapy Administered Upstream to Patients With NSTEMI
Authors: Pollack Charles V. , Peacock W. Frank , Bhandary Durgesh D. , Silber Steven H. , Bhalla Narinder , Rao Sunil V. , Diercks Deborah B. , Frost Alex , Bangalore Sripal , Heitner John F. , Johnson Charles , DeRita Renato , Khan Naeem D. 
Published: September 2020

Objective:
To describe from a noninterventional registry (Utilization of Ticagrelor in the Upstream Setting for Non-ST-Segment Elevation Acute Coronary Syndrome), the short-term ischemic and hemorrhagic outcomes in patients with non-ST elevation myocardial infarction (MI) are managed with a loading dose (LD) of a P2Y12 inhibitor (P2Y12i) given at least 4 hours before diagnostic angiography and delineation of coronary anatomy. Prior data on the effects of such “upstream loading” have been inconsistent.

Methods:
In 53 US hospitals, we evaluated the in-hospital care and outcomes of patients with confirmed non-ST elevation MI managed with an interventional strategy and loaded upstream (at least 4 h before diagnostic angiography) with oral P2Y12i therapy. Patients entered into the database were grouped into 1 of 4 cohorts for analysis: (1) overall cohort, (2) thienopyridine (clopidogrel or prasugrel) load, (3) ticagrelor load, and (4) ticagrelor-consistent. The fourth cohort is a subset of cohort 3 that received ticagrelor throughout the index hospital stay and at discharge. We evaluated in-hospital clinical course and ischemic and bleeding outcomes in all patients and also 30-day outcomes in the ticagrelor-consistent cohort.

Results:
A total of 3355 patients were enrolled, of whom 1087 had 30-day follow-up. The mean (±SD) age was 63.3 ± 12.5 years, and 62.6% were male. Thrombolysis in MI and Global Registry of Acute Coronary Events scores placed these patients in the intermediate risk range, and CRUSADE scores were in the moderate risk range. The LD in Utilization of Ticagrelor in the Upstream Setting for Non-ST-Segment Elevation Acute Coronary Syndrome was clopidogrel in 45.6%, ticagrelor in 53.6%, and prasugrel in 0.8%. The median upstream interval (LD to angiography) was 17:27 hours and did not change appreciably over the course of the data collection period (2/15–10/19). Access was radial in 48.6% and femoral in 51.4%. Postangiography management was medical only in 32.3%, percutaneous coronary intervention in 59.4%, and coronary artery bypass grafting in 8.3%. Median length of stay was 2.7 days, and median time from angiography to coronary artery bypass grafting was 3.6 days. In-hospital mortality was 0.51%, and major bleeding (thrombolysis in MI) was 0.24%; the in-hospital major adverse cardiovascular events rate was 0.7%, and stent thrombosis occurred in 0.18%. No significant differences were seen between the ticagrelor and clopidogrel cohorts in hospital, but 16% received more than 1 P2Y12i in-hospital. On follow-up (93.2% response), 86.7% of patients reported taking ticagrelor as directed.

Conclusions:
Upstream loading of P2Y12i was associated with very low rates of bleeding and short length of stay in a large cohort of non-ST elevation MI (NSTEMI) patients managed invasively.
Establishment of a High‐risk MDS/AML Cell Line YCU‐AML1 and its Xenograft Model Harboring t(3;3) and Monosomy 7
Authors: Kunimoto Hiroyoshi , Fukuchi Yumi , Murakami Koichi , Ikeda Junji , Teranaka Hiroshi , Kato Ikuma , Miyazaki Takuya , Enaka Makiko , Mitsuhashi Takayuki , Yamazaki Etsuko , Kameyama Kaori , Murata Mitsuru , Okamoto Shinichiro , Nakajima Hideaki 
Published: September 2020

AbstractAcute myeloid leukemia (AML) or myelodysplastic syndromes (MDS) with both inv(3)(q21q26.2)/t(3;3)(q21;q26.2) and monosomy 7 defines an extremely aggressive myeloid cancer whose molecular pathogenesis and optimal therapeutic strategy still remain unclear. We established a new MDS/AML cell line, YCU‐AML1, and its patient‐derived xenograft (PDX) model from a high‐risk MDS patient who later transformed into AML harboring both t(3;3)(q21;q26.2) and monosomy 7. YCU‐AML1 cells propagated in co‐culture system with stromal cells in granulocyte macrophage colony‐stimulating factor (GM‐CSF)‐dependent manner. CD34+ bone marrow cells derived from our PDX model showed high EVI1 and low GATA2 expression. Moreover, mutational profile of our MDS/AML model was consistent with recently published mutational spectrum of myeloid malignancies with inv(3)/t(3;3). These data suggest that YCU‐AML1 cells and its MDS/AML model strongly mimics a high‐risk human myeloid cancer with inv(3)(q21q26.2)/t(3;3)(q21;q26.2) and monosomy 7 in terms of both clinical phenotype and molecular basis. We believe our model can be used as a feasible tool to further explore molecular pathogenesis and novel treatment strategy of high‐risk MDS/AML with t(3;3)(q21;q26.2) and monosomy 7.
A Retrospective Study of Corneal Endothelial Dystrophy in Dogs (1991–2014)
Authors: Leonard Brian C. , Kermanian Celine S. , Michalak Sarah R. , Kass Philip H. , Hollingsworth Steven R. , Good Kathryn L. , Maggs David J. , Thomasy Sara M. 
Published: September 2020

Purpose:
To retrospectively evaluate the clinical data, diagnostic tests, treatments, and outcomes for dogs with corneal endothelial dystrophy (CED) and determine risk factors for CED when compared with a canine reference population.

Methods:
Medical records of 99 dogs (1991–2014) diagnosed with CED at the University of California Davis Veterinary Medical Teaching Hospital were reviewed and compared with 458,680 dogs comprising the general hospital population during the study period. Retrieved data included signalment, examination findings, diagnoses, treatments, and outcomes associated with CED. The exact Pearson χ2 test or exact Kruskal–Wallis test was used to compare parameters between the groups. Progression of corneal edema was assessed using 3 independent Kaplan–Meier curves, identifying clinically significant changes in corneal opacity.

Results:
Boston terriers, German wirehaired pointers, and Dachshunds were overrepresented in the CED-affected group, whereas Labradors were underrepresented. Dogs older than 11 years were overrepresented in the CED-affected group, whereas intact dogs were underrepresented. Surgical intervention was performed (n = 11) based on the severity of disease and secondary complications from CED. Median time to progression of corneal edema was 1) 368 days when an at-risk eye initially without edema developed edema at a subsequent visit, 2) 701 days when there was progression from mild to marked corneal edema, and 3) 340 days when there was progression from focal to diffuse corneal edema.

Conclusions:
Many CED-affected dogs progress over months to years without surgical intervention, making dogs with CED a useful model for studying genetic predispositions and development of novel therapeutics for Fuchs endothelial corneal dystrophy.