bluuebunny/crossref_metadata_2025_split huggingface dataset:
Best practice guidelines for management of spinal disorders in skeletal dysplasia
Authors: White Klane K. , Bober Michael B. , Cho Tae-Joon , Goldberg Michael J. , Hoover-Fong Julie , Irving Melita , Kamps Shawn E. , Mackenzie William G. , Raggio Cathleen , Spencer Samantha A. , Bompadre Viviana , Savarirayan Ravi ,   on behalf of the Skeletal Dysplasia Management Consortium
Published: June 2020

Abstract
Background
Disorders of the spine present a common and difficult management concern in patients with skeletal dysplasia. Due to the rarity of these conditions however, the literature, largely consisting of small, single institution case series, is sparse in regard to well-designed studies to support clinical decision making in these situations.

Methods
Using the Delphi method, an international, multi-disciplinary group of individuals, with significant experience in the care of patients with skeletal dysplasia, convened to develop multi-disciplinary, “best practice” guidelines in the care of spinal disorders in patients with skeletal dysplasia.

Results
Starting with 33 statements, the group a developed a list of 31 “best practice” guidelines.

Conclusions
The guidelines are presented and discussed to provide context for clinicians in their decision making in this often-challenging realm of care.
Single-cell transcriptome and antigen-immunoglobin analysis reveals the diversity of B cells in non-small cell lung cancer
Authors: Chen Jian , Tan Yun , Sun Fenghuan , Hou Likun , Zhang Chi , Ge Tao , Yu Huansha , Wu Chunxiao , Zhu Yuming , Duan Liang , Wu Liang , Song Nan , Zhang Liping , Zhang Wei , Wang Di , Chen Chang , Wu Chunyan , Jiang Gening , Zhang Peng 
Published: June 2020

Abstract
Background
Malignant transformation and progression of cancer are driven by the co-evolution of cancer cells and their dysregulated tumor microenvironment (TME). Recent studies on immunotherapy demonstrate the efficacy in reverting the anti-tumoral function of T cells, highlighting the therapeutic potential in targeting certain cell types in TME. However, the functions of other immune cell types remain largely unexplored.

Results
We conduct a single-cell RNA-seq analysis of cells isolated from tumor tissue samples of non-small cell lung cancer (NSCLC) patients, and identify subtypes of tumor-infiltrated B cells and their diverse functions in the progression of NSCLC. Flow cytometry and immunohistochemistry experiments on two independent cohorts confirm the co-existence of the two major subtypes of B cells, namely the naïve-like and plasma-like B cells. The naïve-like B cells are decreased in advanced NSCLC, and their lower level is associated with poor prognosis. Co-culture of isolated naïve-like B cells from NSCLC patients with two lung cancer cell lines demonstrate that the naïve-like B cells suppress the growth of lung cancer cells by secreting four factors negatively regulating the cell growth. We also demonstrate that the plasma-like B cells inhibit cancer cell growth in the early stage of NSCLC, but promote cell growth in the advanced stage of NSCLC. The roles of the plasma-like B cell produced immunoglobulins, and their interacting proteins in the progression of NSCLC are further validated by proteomics data.

Conclusion
Our analysis reveals versatile functions of tumor-infiltrating B cells and their potential clinical implications in NSCLC.
The relationship between hyperuricemia and contrast-induced acute kidney injury undergoing primary percutaneous coronary intervention: secondary analysis protocol for the ATTEMPT RESCIND-1 study
Authors: Guo Wei , Song Feier , Chen Shiqun , Zhang Li , Sun Guoli , Liu Jin , Chen Jiyan , Liu Yong , Tan Ning ,   the RESCIND group
Published: June 2020

Abstract
Background
Contrast-induced acute kidney injury (CI-AKI) contributes toward unfavorable clinical outcomes after primary percutaneous coronary intervention (pPCI). We will assess whether hyperuricemia is an independent predictor of CI-AKI and outcomes in patients undergoing pPCI.

Methods/design
Our study is a secondary analysis for the database from ATTEMPT study, enrolling 560 ST-segment elevation myocardial infarction (STEMI) patients undergoing pPCI. Patients will be divided into 2 groups according to the admission serum uric acid (SUA) level. Hyperuricemia will be defined as a SUA level > 7 mg/dL (417 mmol/L) in males and > 6 mg/dL (357 mmol/L) in females. The primary endpoint was CI-AKI, defined as > 25% or 0.5 mg/dL increase in serum creatinine from baseline during the first 48–72 h post-procedurally. Multivariate analyses for CI-AKI and long-term mortality will be performed using the logistic regression and Cox regression analyses, respectively.

Discussion
This study will determine the predictive value of hyperuricemia for the development of CI-AKI and outcomes in patients with STEMI undergoing pPCI. We predict that hyperuricemia will be associated with a risk of CI-AKI in patients with pPCI. Furthermore, after adjusting for other variables, long-term mortality after pPCI may be higher in those with hyperuricemia than in those with normouricemia. Results of this study may provide scientific evidence for the effect of hyperuricemia on CI-AKI and long-term outcomes, thereby offering the potential possibility of lowering SUA on the development of CI-AKI and outcomes.

Trial registration
ClinicalTrials.gov NCT02067195, Registered on 20 February 2014.
Life in mine tailings: microbial population structure across the bulk soil, rhizosphere, and roots of boreal species colonizing mine tailings in northwestern Québec
Authors: Gagnon Vanessa , Rodrigue-Morin Michaël , Tremblay Julien , Wasserscheid Jessica , Champagne Julie , Bellenger Jean-Philippe , Greer Charles W. , Roy Sébastien 
Published: June 2020

Abstract
Purpose
Mining activities have negative effects on soil characteristics and can result in low pH, high heavy metal content, and limited levels of essential nutrients. A tailings storage area located in northwestern Québec showed natural colonization by plants from the adjacent natural environment. The objective of the study was to determine the main edaphic parameters that structured microbial populations associated with the indigenous woody plants that had naturally colonized the site.

Methods
Microbial populations were studied in the bulk soil, the rhizosphere, and inside plant roots using Illumina sequencing, ordination analysis (i.e., redundancy analysis (RDA) and principal coordinates analysis (PCoA)), ternary plotting, and statistical analysis (MANOVA).

Results
The main variables that drove the microbial community patterns were plant species and the tailings pH. Indeed, the main bacterial classes were Gammaproteobacteria and Deltaproteobacteria in both the rhizosphere and root endosphere. Analysis revealed that some dominant operational taxonomic units (e.g., Pseudomonas sp., Acinetobacter sp., and Delftia sp.) were present in increased proportions in roots for each plant species under study. This study also revealed that many of the most abundant fungal genera (e.g., Claussenomyces, Eupenicillium, and Trichoderma) were more abundant in the rhizosphere than in the root endosphere.

Conclusions
This comprehensive study of the microbial community dynamics in the bulk soil, rhizosphere, and root endosphere of boreal trees and shrubs could be beneficial in facilitating the rehabilitation of disturbed ecosystems.
Rapid identification and detection of Vibrio parahaemolyticus via different types of modus operandi with LAMP method in vivo
Authors: Li Jun , Liu Jianfei , Fu Kaifei , Qin Kewei , Wu Chenglin , Yu Xiaojie , Zhou Shan , Zhou Lijun 
Published: June 2020

Abstract
Purpose
Vibrio parahaemolyticus, an easy-ignored food-borne pathogen, can cause bacterial outbreaks and human disease during early-stage infection. In this study, we aimed to evaluate the detection efficiency of loop-mediated isothermal amplification (LAMP) as an emerging technique to directly detect V. parahaemolyticus infection in mammalian hosts and assess its potential in clinical applications.

Methods
A LAMP assay was used for rapid identification of V. parahaemolyticus in a variety of mouse models in which animals were infected via the digestive tract, wounds, or through general infection, and the results were compared with routine analytical methods.

Results
Our results confirmed that the LAMP assay was capable of detecting V. parahaemolyticus in different mouse organs independent of the source of bacteria, although its sensitivity depended on the route of infection and the organ affected. Foodborne-derived V. parahaemolyticus was the most sensitive route, with the small intestine being the most sensitive organ. The LAMP assay indicated that V. parahaemolyticus that spread through the blood stream had the most serious consequences during early-stage infection. Positive LAMP results were identified in all blood samples from i.v. injected mice. Furthermore, the LAMP method could directly detect trace quantities of V. parahaemolyticus in fresh peripheral blood while conventional methods failed to do so, thereby shortening the time-to-result from days to minutes.

Conclusions
In this study, we demonstrated that the LAMP assay was effective in speeding up the detection of V. parahaemolyticus. Instead of being a secondary method to assist in the clinic, the LAMP assay has potential for use as the primary technique for rapid detection of V. parahaemolyticus in the future.
Arthroscopically-assisted reduction and pinning of a radial neck fracture in a child: a case report and review of the literature
Authors: Colozza Alessandra , Padovani Sara , Caruso Gaetano , Cavaciocchi Michele , Massari Leo 
Published: June 2020

Abstract
Background
Displaced radial neck fractures in children are challenging to treat. The age of the patient and the degree of angulation are the main criteria to consider when managing these fractures in children. Various surgical techniques have been described in the literature for both indirect and direct reduction and for fixation. However, the best treatment is still debated.

Case presentation
The case presented is of a 6-year-old Caucasian boy with an impacted and displaced radial neck fracture. With the patient in lateral position, under general anesthesia, elbow arthroscopy was performed to better visualize the articular surface and to assist with reduction and fixation. The fracture was reduced and fixed with a single K-wire under direct arthroscopic visualization. No associated lesions were found. An above-elbow cast was applied after surgery. The cast and K-wire were removed 3 weeks later. At the 3-month follow-up, the patient showed a full recovery with complete range of movement without any postoperative and radiographic complications.

Conclusion
Traditionally, surgery for displaced radial neck fractures in children is performed by closed reduction with percutaneous pinning or elastic intramedullary nail fixation under fluoroscopic guidance. Direct visualization of the articular surface via an open approach allows better reduction in complex fracture patterns but is related to a higher risk of complications: elbow stiffness, instability, or avascular necrosis. Elbow arthroscopy in children could be a valid alternative to open fixation surgery for displaced radial neck fractures without the complications associated with articular exposure, allowing the direct visualization of the fracture and reducing radiation exposure. Although technically demanding, we believe elbow arthroscopy should be considered an alternative option because it is effective in assisting reduction and fixation and enables the detection of associated joint lesions.
Multipoint channel charting-based radio resource management for V2V communications
Authors: Al-Tous Hanan , Ponnada Tushara , Studer Christoph , Tirkkonen Olav 
Published: June 2020

AbstractWe consider a multipoint channel charting (MPCC) algorithm for radio resource management (RRM) in vehicle-to-vehicle (V2V) and vehicle-to-infrastructure (V2I) communication systems. A massive MIMO (mMIMO) infrastructure network performs logical localization of vehicles to a MPCC, based on V2I communication signals. Combining logical distances given by channel charting with V2V measurements, the network trains a function to predict the quality of a direct V2V communication link from observed V2I communication signals. In MPCC, the network uses machine learning techniques to learn a logical radio map from V2I channel state information (CSI) samples transmitted from unknown locations. The network extracts CSI features, constructs a dissimilarity matrix between CSI samples, and performs dimensional reduction of the CSI feature space. Here, we use Laplacian Eigenmaps (LE) for dimensional reduction. The resulting MPCC is a two-dimensional map where the spatial distance between a pair of vehicles is closely approximated by the distance in the MPCC. In addition to V2I CSI, the network acquires V2V channel quality information for vehicles in the training set and develops a link quality predictor. MPCC provides a mapping for any vehicle location in the training set. To use MPCC for cognitive RRM of V2I and V2V communications, network management has to find logical MPCC locations for vehicles not in the training set, based on newly acquired V2I CSI measurements. For this, we develop an extension of LE-based MPCC to out-of-sample CSI samples. We evaluate the performance of link quality prediction for V2V communications in a mMIMO millimeter-wave scenario, in terms of the relative error of the predicted outage probability.
Patient and healthcare provider reported barriers and enablers to virtual or remote-only follow-up models for cardiovascular implantable electronic devices: protocol for a qualitative framework synthesis
Authors: Kelly Shannon E. , Clifford Tammy J. , Skidmore Becky , Birnie David , Parkash Ratika , Wells George A. 
Published: June 2020

AbstractBackgroundVirtual care models are used to follow-up patients with cardiovascular implantable electronic devices (CIED), including pacemakers, implantable cardioverter defibrillators, and cardiac resynchronization therapy. There is increasing interest in the expansion of virtual, or even remote-only, CIED care models to alleviate resource and economic burden to both patients and specialty device clinics and to maintain or improve equity and access to high-quality cardiovascular care. This qualitative framework synthesis aims to identify barriers and enablers to virtual care models from both the perspective of the patient and device clinics. How setting, context, equity factors or other aspects influence these factors, or satisfaction with care, will also be investigated.MethodsWe will perform a systematic literature search in MEDLINE, Embase, PsycINFO, CINAHL, Proquest Dissertations & Theses, other EBM Reviews, and trial registry databases. Screening will be completed by two independent review authors. Original research articles having a qualitative component (i.e., qualitative, mixed-, or multi-method) are eligible. Study populations of interest are (a) individuals with a CIED or (b) healthcare providers involved in any aspect of virtual or remote follow-up of patients with CIEDs. Eligibility will be restricted to studies published after January 1, 2000 in English or French. Data will be captured using standardized templates based on the domains and constructs of the Theoretical Domains Framework and the Warwick Patient Experiences Framework. The Joanna Briggs Institute Critical Appraisal Checklist for Qualitative Research will be applied to all included studies. The GRADE-CERQual approach will be applied to assess and summarize confidence in key findings. Reporting will follow the enhancing transparency in reporting the synthesis of qualitative research (ENTREQ) statement. Detailed descriptive results will be presented, and summary of qualitative findings tables will be produced.DiscussionWhile a number of trials have captured the clinical effectiveness and safety of virtual follow-up for CIEDs, there has been less attention given to factors affecting use and implementation of remote care by patients and healthcare providers or satisfaction with care. Results from this qualitative framework synthesis will provide important lived experience data from both patients and healthcare providers which will be essential to incorporate in clinical guidelines.Systematic review registrationPROSPEROCRD42020160533.
Geotechnical investigations of an earthquake that triggered disastrous landslides in eastern Canada about 1020 cal BP
Authors: Wang Baolin 
Published: June 2020

AbstractGeotechnical studies are carried out to investigate the location and magnitude of a prehistoric earthquake in eastern Canada. Previous studies identified 12 sensitive clay landslides of about 1020 cal BP in the Ottawa-Gatineau region, including one of the most disastrous slope failures in eastern Canada. The landslides were hypothesized to have been triggered by an earthquake. In the current study, three of those landslides are investigated to understand their failure mechanisms. The study sites form a triangle of lateral distances of about 38, 38 and 35 km. Micro-seismic surveys, cone penetrometer tests, vane shear tests, soil sampling and laboratory testing are conducted to collect geotechnical data. Slope stability models are constructed to calculate threshold ground accelerations required to trigger the landslides. The ground accelerations are used with Ground-Motion Prediction Equations to triangulate the earthquake. The results indicate an earthquake of a minimum moment magnitude of 5.9 near Eardley, Quebec.
Patient involvement in preparing health research peer-reviewed publications or results summaries: a systematic review and evidence-based recommendations
Authors: Arnstein Lauri , Wadsworth Anne Clare , Yamamoto Beverley Anne , Stephens Richard , Sehmi Kawaldip , Jones Rachel , Sargent Arabella , Gegeny Thomas , Woolley Karen L. 
Published: June 2020

Abstract
Background
There are increasing calls for patient involvement in sharing health research results, but no evidence-based recommendations to guide such involvement. Our objectives were to: (1) conduct a systematic review of the evidence on patient involvement in results sharing, (2) propose evidence-based recommendations to help maximize benefits and minimize risks of such involvement and (3) conduct this project with patient authors.

Methods
To avoid research waste, we verified that no systematic reviews were registered or published on this topic. We co-created, with patients, a PRISMA-P–compliant protocol. We included peer-reviewed publications reporting the effects of patient involvement in preparing peer-reviewed publications or results summaries from health research studies. We searched (9/10/2017) MEDLINE, EMBASE and the Cochrane Database of Systematic Reviews, and secondary information sources (until 11/06/2018). We assessed the risk of bias in eligible publications and extracted data using standardized processes. To evaluate patient involvement in this project, we co-created a Patient Authorship Experience Tool.

Results
All nine eligible publications reported on patient involvement in preparing publications; none on preparing results summaries. Evidence quality was moderate. A qualitative synthesis of evidence indicated the benefits of patient involvement may outweigh the risks. We have proposed 21 evidence-based recommendations to help maximize the benefits and minimize the risks when involving patients as authors of peer-reviewed publications. The recommendations focus on practical actions patient and non-patient authors can take before (10 recommendations), during (7 recommendations) and after (4 recommendations) manuscript development. Using the Patient Authorship Experience Tool, both patient and non-patient authors rated their experience highly.

Conclusions
Based on a systematic review, we have proposed 21 evidence-based recommendations to help maximize the benefits and minimize the risks of involving patients as authors of peer-reviewed publications.
Divergent differentiation and variant morphology in invasive urothelial carcinomas – association with muscle-invasive disease
Authors: Santana Suelen Cunha , de Souza Maiara Ferreira , Amaral Maria Estela Pompeu , Athanazio Daniel Abensur 
Published: June 2020

Abstract
Introduction
To evaluate the frequency of divergent differentiations / variant morphology in urothelial carcinoma, and their association with muscle-invasive disease at diagnosis.

Methods
All consecutive cases of invasive urothelial carcinoma from a busy pathology laboratory were reviewed. Clinical and pathological data were recorded including data on divergent and variant morphologies and their percentage within the invasive component.

Results
Among 91 cases, 46 (51%) showed some form of divergent/variant morphology. The most common divergent morphology was squamous which was present in 18/46 (39% of cases with some divergent or variant morphology) followed by micropapillary (28%), plasmacytoid (20%) and poorly differentiated (17%). Only squamous differentiation was associated with higher rate with muscularis propria invasion.

Conclusions
Although common, squamous differentiation should be still recognized as a feature of aggressive disease.
Perceived organizational support and job satisfaction: a moderated mediation model of proactive personality and psychological empowerment
Authors: Maan Annum Tariq , Abid Ghulam , Butt Tahira Hassan , Ashfaq Fouzia , Ahmed Saira 
Published: June 2020

AbstractDrawing on social exchange theory, the purpose of this study is to examine the mediating role of psychological empowerment and moderating role of proactive personality in the relationship between POS and job satisfaction. The data were collected from 936 employees working in various manufacturing and service sectors by using self-report survey questionnaires by employing time-lagged cross-sectional study design. The study findings demonstrate that POS positively influenced psychological empowerment and job satisfaction. Moreover, it is also revealed that the relationship between POS and job satisfaction is weaker when employees’ proactive personality is higher rather than lower. The findings of the current study pose a framework for organizational representatives of both service and manufacturing industries to strengthen individual psychological empowerment and job satisfaction by offering organizational support to those individuals who are less proactive.
The pattern of neonatal gastro-intestinal perforation in upper Egypt
Authors: Abo-Halawa Nezar Abd Elrouf , Negm Mohamed Ahmed , Fathy Mohamed 
Published: June 2020

AbstractBackgroundNeonatal gastro-intestinal perforation [NGIP] is one of the major problems in pediatric surgical practice. Although the outcomes of neonatal surgery have improved markedly over the past decade the mortality rates of neonates with NGIP are still high. The aim of this study was to present the possible etiological factors, clinical findings, and operative procedures of NIP in our locality.ResultsA total of 34 neonates with NGIP were included in this study. The median age at presentation was (15.8 ± 7.0 SD) days. The median interval between presentation and surgical interference was (2.0 ± 1.1 SD).Necrotizing enterocolitis [NEC] was the commonest cause of neonatal gastro-intestinal perforation. The commonest site of perforation was the colon [11cases]. The overall mortality rate was 11 cases [32.4%]. The main cause of mortality was neonatal NEC [6 cases]. Eight cases [40 %] died out of 20 cases which the interval between the presentation and interference were more than one day.ConclusionsNeonatal gastro-intestinal perforations are still associated with high mortality rate in our institutions, and delayed diagnosis with increased interval between the presentation and surgical intervention are associated with increased mortality. In our locality, although NEC is the commonest cause of NGIP, the iatrogenic cause is relatively higher than reported.