bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel use of culturomics to identify the microbiota in hospital sink drains with and without persistent VIM-positive Pseudomonas aeruginosa
Authors: Pirzadian Jannette , Harteveld Susan P. , Ramdutt Shanice N. , van Wamel Willem J. B. , Klaassen Corné H. W. , Vos Margreet C. , Severin Juliëtte A. 
Published: October 2020

AbstractIn hospitals, Verona Integron-encoded Metallo-beta-lactamase (VIM)-positive Pseudomonas aeruginosa may colonize sink drains, and from there, be transmitted to patients. These hidden reservoirs are difficult to eradicate since P. aeruginosa forms biofilms that resist disinfection. However, little is known on the composition of these biofilms. Therefore, culturomics was used for the first time to investigate the viable microbiota in four hospital sink drain samples with longstanding VIM-positive P. aeruginosa drain reservoirs (inhabited by high-risk clone, sequence type ST111), and four drain samples where VIM-positive P. aeruginosa was not present. Microbial load and composition varied between samples, yielding between 471–18,904 distinct colonies and 8–20 genera. In two VIM-positive drain samples, P. aeruginosa was the most abundantly-isolated microorganism, and found in combination with other Gram-negative bacteria, Citrobacter, Enterobacter, or Stenotrophomonas. P. aeruginosa was in low abundance in the other two VIM-positive samples, and found with Gram-positive cocci (Enterococcus and Staphylococcus) or Sphingomonas. In VIM-negative drain samples, high abundances of Gram-negative non-fermenting bacteria, including Acinetobacter, non-aeruginosa Pseudomonas spp., Acidovorax, Chryseobacterium, Flavobacterium, and Sphingobium, as well as Candida, were cultured. Although additional experiments are needed to draw more firm conclusions on which microorganisms enable or inhibit VIM-positive P. aeruginosa persistence, our data provide unique insights into the microbial compositions of sink drain inlets.
Most abundant metabolites in tissues of freshwater fish pike-perch (Sander lucioperca)
Authors: Tsentalovich Yuri P. , Zelentsova Ekaterina A. , Yanshole Lyudmila V. , Yanshole Vadim V. , Odud Iliya M. 
Published: October 2020

AbstractQuantitative metabolomic analysis was performed for eleven tissues of freshwater fish pike-perch (Sander lucioperca), including gill, heart, liver, kidney, spleen, muscle, brain, milt, lens, aqueous (AH) and vitreous (VH) humors with the use of NMR spectroscopy. The absolute values of concentrations were determined for more than 65 most abundant metabolites in every tissue. It was found that from the metabolomic viewpoint, kidney and gill are the most similar tissues, while the metabolomic compositions of ocular tissues—lens, AH, and VH significantly differ from that of other tissues. The combinations of intracellular osmolytes and antioxidants are specific for every tissue. In particular, the concentration of antioxidant ovothiol A in the lens is much higher than in any other tissue, while the brain enjoys the elevated level of ascorbate. The most abundant osmolyte in the fish spleen, muscle, and heart is taurine, and in the brain, gill, and lens—myo-inositol. Other important osmolytes specific for particular tissues are N-acetyl-histidine, N-acetyl-aspartate, betaine, threonine-phosphoethanolamine, and serine-phosphoethanolamine. The quantitative data obtained in the present work can be used as the baseline metabolite concentrations in the fish tissues to evaluate the influence of seasonal, ecological and other factors on the fish metabolism.
Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35
Authors: Dausset Caroline , Bornes Stéphanie , Miquel Sylvie , Kondjoyan Nathalie , Angenieux Magaly , Nakusi Laurence , Veisseire Philippe , Alaterre Elina , Bermúdez-Humarán Luis G. , Langella Philippe , Engel Erwan , Forestier Christiane , Nivoliez Adrien 
Published: October 2020

AbstractGYNOPHILUS (Lcr REGENERANS) is a live biotherapeutic product (LBP) aimed at restoring the vaginal microbiome and contains the live biotherapeutic microorganism Lactobacillus rhamnosus Lcr35. In this study, the LBP formulation and manufacturing process significantly enhanced the anti-Candida activity of L. rhamnosus Lcr35, with a complete loss of viability of the yeast after 48 h of coincubation. Sodium thiosulfate (STS), one excipient of the product, was used as a potentiator of the anti-Candida spp. activity of Lactobacilli. This contact-independent phenomenon induced fungal cell disturbances, as observed by electron microscopy observations. Nonverbal sensory experiments showed clear odor dissimilarities between cocultures of L. rhamnosus Lcr35 and C. albicans in the presence and absence of STS, suggesting an impact of odor-active metabolites. A volatolomic approach allowed the identification of six odor-active compounds, including one sulfur compound that was identified as S-methyl thioacetate (MTA). MTA was associated with the antifungal effect of Lcr35, and its functional link was established in vitro. We show for the first time that the LBP GYNOPHILUS, which is a highly active product in the reduction of vulvovaginal candidiasis, requires the presence of a sulfur compound to fully achieve its antifungal effect.
Analysis of expression, epigenetic, and genetic changes of HNF1B in 130 kidney tumours
Authors: Bártů Michaela , Hojný Jan , Hájková Nikola , Michálková Romana , Krkavcová Eva , Hadravský Ladislav , Kleissnerová Lenka , Bui Quang Hiep , Stružinská Ivana , Němejcová Kristýna , Čapoun Otakar , Šlemendová Monika , Dundr Pavel 
Published: October 2020

AbstractHepatocyte nuclear factor 1 beta (HNF1B) is a transcription factor which plays a crucial role in nephronogenesis, and its germline mutations have been associated with kidney developmental disorders. However, the effects of HNF1B somatic exonic mutations and its role in the pathogenesis of kidney tumours has not yet been elucidated. Depending on the type of the tumour HNF1B may act as a tumour suppressor or oncogene, although the exact mechanism by which HNF1B participates in the process of cancerogenesis is unknown. Using an immunohistochemical approach, and methylation and mutation analysis, we have investigated the expression, epigenetic, and genetic changes of HNF1B in 130 cases of renal tumours (121 renal cell carcinomas, 9 oncocytomas). In the subset of clear cell renal cell carcinoma (ccRCC), decreased HNF1B expression was associated with a higher tumour grade and higher T stage. The mutation analysis revealed no mutations in the analysed samples. Promoter methylation was detected in two ccRCCs and one oncocytoma. The results of our work on a limited sample set suggest that while in papillary renal cell carcinoma HNF1B functions as an oncogene, in ccRCC and chRCC it may act in a tumour suppressive fashion.
PAK4 methylation by the methyltransferase SETD6 attenuates cell adhesion
Authors: Vershinin Zlata , Feldman Michal , Levy Dan 
Published: October 2020

AbstractP21-activated kinase 4 (PAK4), a member of serine/threonine kinases family is over-expressed in numerous cancer tumors and is associated with oncogenic cell proliferation, migration and invasion. Our recent work demonstrated that the SET-domain containing protein 6 (SETD6) interacts with and methylates PAK4 at chromatin in mammalian cells, leading to activation of the Wnt/β-catenin signaling pathway. In our current work, we identified lysine 473 (K473) on PAK4 as the primary methylation site by SETD6. Methylation of PAK4 at K473 activates β-catenin transcriptional activity and inhibits cell adhesion. Specific methylation of PAK4 at K473 also attenuates paxillin localization to focal adhesions leading to overall reduction in adhesion-related features, such as filopodia and actin structures. The altered adhesion of the PAK4 wild-type cells is accompanied with a decrease in the migrative and invasive characteristics of the cells. Taken together, our results suggest that methylation of PAK4 at K473 plays a vital role in the regulation of cell adhesion and migration.
Poly (ADP-ribose) polymerase inhibitor exposure reduces ovarian reserve followed by dysfunction in granulosa cells
Authors: Nakamura Kentaro , Takae Seido , Shiraishi Eriko , Shinya Kiemi , Igualada Arby Jane , Suzuki Nao 
Published: October 2020

AbstractThe use of poly (ADP-ribose) polymerase (PARP) inhibitors is expected to increase, but their effect on fertility is still unclear. The aim of this study was to investigate the effect of PARP inhibitors on ovarian function. In an in vitro study, cultures of ovaries and granulosa cells (GCs) exposed to the PARP inhibitor olaparib were evaluated by real-time RT-PCR, histological study, and hormone assays. In an in vivo study, mice were administered olaparib orally and evaluated via in vitro fertilization (IVF), follicle count, immunohistochemical staining, and real-time RT-PCR. In vitro, the gene expression of GC markers decreased in the olaparib-treated group. Olaparib also negatively affected estradiol production and the expression of GC markers in cultured GCs, with abnormal morphology of GCs observed in the treated group. The follicle number indicated depletion of follicles due to atretic changes in the treatment group, both in vitro and in vivo. Also, olaparib reduced the number of retrieved oocytes and the fertilization rate of IVF, but they recovered after 3 weeks of cessation. Our results indicate that olaparib is toxic to ovaries.
A model study of the combined effect of above and below ground plant traits on the ecomorphodynamics of gravel bars
Authors: Caponi Francesco , Vetsch David F. , Siviglia Annunziato 
Published: October 2020

AbstractBoth above- and below-ground plant traits are known to modulate feedbacks between vegetation and river morphodynamic processes. However, how they collectively influence vegetation establishment on gravel bars remains less clear. Here we develop a numerical model that couples above- and below-ground vegetation dynamics with hydromorphological processes. The model dynamically links plant growth rate to water table fluctuations and includes plant mortality by uprooting and burial. We considered a realistic hydrological regime and used the model to simulate the coevolution of alternate gravel bars and vegetation that displays trade-offs in investment of above- and below-ground biomass. We found that a balanced plant growth above- and below-ground facilitates vegetation to establish on steady, stable bars, because it allows plants to develop traits that maximise growth performance during low flow periods and thus survival during floods. Regardless of the growth strategy, vegetation could not establish on migrating bars because of large plant loss by uprooting during floods. These findings add on previous studies suggesting that morphodynamic processes play a key role on determining plant trait distributions and highlight the importance of including the dynamics of both above- and below-ground plant traits for predicting shifts between bare and vegetated states in river bars.
Fluoroless and contrast-free catheter ablation without a lead apron in routine clinical practice
Authors: Matsubara Takumi J. , Fujiu Katsuhito , Shimizu Yu , Oshima Tsukasa , Matsuda Jun , Matsunaga Hiroshi , Oguri Gaku , Hasumi Eriko , Kojima Toshiya , Komuro Issei 
Published: October 2020

AbstractThe technique of catheter ablation has been improved within the past few decades, especially by three-dimensional (3D) mapping system. 3D mapping system has reduced radiation exposure but ablation procedures still require fluoroscopy. Our previous study showed the safety and efficacy of catheter ablation based on intracardiac echogram combined with CARTOSOUND/CARTO3 system, however fluoroscopy use for an average of 16 min is required for this procedure. The present study was aimed to reduce radiation exposure to zero and establish a radiation free catheter ablation method with the goal of utilizing it in routine clinical practice. We conducted single center, retrospective study during 2019 April to 2020 February. Consecutive 76 patients were enrolled. In the first 18 cases, the previously reported procedure (CARTOSOUND/CARTO3 method) was used. The remaining 58 cases were transitioned to fluoroless catheter ablation. The procedure time, success rates and complication rates were analyzed. Not only AF patients but atrial flutter (AFL), paroxysmal supraventricular tachycardia (PSVT) and ventricular arrhythmia patients were included. Catheter positioning, catheter visualization and collecting the geometry of each camber of the heart were conducted by using contact force and ICE based geometry on CARTO system without either prior computed tomography (CT) or magnetic resonance image (MRI). In fluoroless group, all catheter ablations were successfully performed without lead aprons. No complications occurred in either group. There were no significant differences in procedure time in any type of procedure (Total procedure time Fluoro-group; 149 ± 51 min vs. Fluoroless-group; 162 ± 43 min, N.S.), (PSVT 170 ± 53 min vs. 162 ± 29 min, N.S.), (AFL 110 ± 70 min vs. 123 ± 43 min, N.S.), (AF 162 ± 43 min vs. 163 ± 32 min, N.S.). The total radiation time was reduced to zero in fluoroless group. Catheter ablation with ICE and 3D mapping system guide without fluoroscopy could be safely performed with a high success rate, without any prior CT/MRI 3D images. Radiation was reduced completely for patients and staff, negating the need for protective wear for operators.
Vicarious conditioned fear acquisition and extinction in child–parent dyads
Authors: Marin Marie-France , Bilodeau-Houle Alexe , Morand-Beaulieu Simon , Brouillard Alexandra , Herringa Ryan J. , Milad Mohammed R. 
Published: October 2020

AbstractThe biological mechanisms involved in fear transmission within families have been scarcely investigated in humans. Here we studied (1) how children acquired conditioned fear from observing their parent, or a stranger, being exposed to a fear conditioning paradigm, and (2) the subsequent fear extinction process in these children. Eighty-three child-parent dyads were recruited. The parent was filmed while undergoing a conditioning procedure where one cue was paired with a shock (CS + Parent) and one was not (CS −). Children (8 to 12 years old) watched this video and a video of an adult stranger who underwent conditioning with a different cue reinforced (CS + Stranger). Children were then exposed to all cues (no shocks were delivered) while skin conductance responses (SCR) were recorded. Children exhibited higher SCR to the CS + Parent and CS + Stranger relative to the CS −. Physiological synchronization between the child’s SCR during observational learning and the parent’s SCR during the actual process of fear conditioning predicted higher SCR for the child to the CS + Parent. Our data suggest that children acquire fear vicariously and this can be measured physiologically. These data lay the foundation to examine observational fear learning mechanisms that might contribute to fear and anxiety disorders transmission in clinically affected families.
Biogenic and chemically synthesized Solanum tuberosum peel–silver nanoparticle hybrid for the ultrasonic aided adsorption of bromophenol blue dye
Authors: Akpomie Kovo G. , Conradie Jeanet 
Published: October 2020

AbstractThis work was aimed at the synthesis of a hybrid (STpe-AgNP), obtained by impregnation of silver nanoparticles (AgNP) onto Solanum tuberosum peel (STpe), for the ultrasonic assisted adsorption of bromophenol blue (BB) dye. SEM, FTIR, XRD, EDX, TGA and BET techniques were used to characterize the adsorbents. The XRD, SEM and EDX confirmed successful impregnation of AgNPs onto STpe to form the hybrid. The AgNPs impregnated onto the hybrid were found to be water stable at various pH values of 2.0–9.0. Chi-square (χ2 < 0.024) and linear regression (R2 > 0.996) showed that the Freundlich model was best fitted among the isotherm models, corroborated by the oriented site model. Kinetic analysis conformed to the intraparticle diffusion and pseudo-first-order rate equations, while thermodynamics displayed a physical, spontaneous and endothermic adsorption process. The presence of competing Pb(II), Ni(II), Cd(II) and Zn(II) metal ions in solution interfered with the adsorption of BB onto the biosorbents. In terms of reusability, STpe and STpe-AgNP showed BB desorption of 91.3% and 88.5% respectively, using NaOH as eluent. Ultra-sonication significantly enhanced the adsorption of BB by both adsorbents, but the impregnation of AgNPs only slightly improved adsorption of the dye from the simulated wastewater. This study also illustrated that pristine STpe biomass waste is a cheap viable option for the decontamination of BB from water.
Wettability and confinement size effects on stability of water conveying nanotubes
Authors: Shaat M. , Javed U. , Faroughi S. 
Published: October 2020

AbstractThis study investigates the wettability and confinement size effects on vibration and stability of water conveying nanotubes. We present an accurate assessment of nanotube stability by considering the exact mechanics of the fluid that is confined in the nanotube. Information on the stability of nanotubes in relation to the fluid viscosity, the driving force of the fluid flow, the surface wettability of the nanotube, and the nanotube size is missing in the literature. For the first time, we explore the surface wettability dependence of the nanotube natural frequencies and stability. By means of hybrid continuum-molecular mechanics (HCMM), we determined water viscosity variations inside the nanotube. Nanotubes with different surface wettability varying from super-hydrophobic to super-hydrophilic nanotubes were studied. We demonstrated a multiphase structure of nanoconfined water in nanotubes. Water was seen as vapor at the interface with the nanotube, ice shell in the middle, and liquid water in the nanotube core. The average velocity of water flow in the nanotube was obtained strongly depend on the surface wettability and the confinement size. In addition, we report the natural frequencies of the nanotube as functions of the applied pressure and the nanotube size. Mode divergence and flutter instabilities were observed, and the activation of these instabilities strongly depended on the nanotube surface wettability and size. This work gives important insights into understanding the stability of nanotubes conveying fluids depending on the operating pressures and the wettability and size of confinement. We revealed that hydrophilic nanotubes are generally more stable than hydrophobic nanotubes when conveying fluids.
Gaia18aen: First symbiotic star discovered by Gaia
Authors: Merc J. , Mikołajewska J. , Gromadzki M. , Gałan C. , Iłkiewicz K. , Skowron J. , Wyrzykowski Ł. , Hodgkin S. T. , Rybicki K. A. , Zieliński P. , Kruszyńska K. , Godunova V. , Simon A. , Reshetnyk V. , Lewis F. , Kolb U. , Morrell M. , Norton A. J. , Awiphan S. , Poshyachinda S. , Reichart D. E. , Greet M. , Kolgjini J. 
Published: October 2020

Context. Besides the astrometric mission of the Gaia satellite, its repeated and high-precision measurements also serve as an all-sky photometric transient survey. The sudden brightenings of the sources are published as Gaia Photometric Science Alerts and are made publicly available, allowing the community to photometrically and spectroscopically follow up on the object.
Aims. The goal of this paper is to analyze the nature and derive the basic parameters of Gaia18aen, a transient detected at the beginning of 2018. This object coincides with the position of the emission-line star WRAY 15-136. The brightening was classified as a “nova?” on the basis of a subsequent spectroscopic observation.
Methods. We analyzed two spectra of Gaia18aen and collected the available photometry of the object covering the brightenings in 2018 and also the preceding and following periods of quiescence. Based on this observational data, we derived the parameters of Gaia18aen and discussed the nature of the object.
Results. Gaia18aen is the first symbiotic star discovered by Gaia satellite. The system is an S-type symbiotic star and consists of an M giant of a slightly super-solar metallicity, where Teff ∼ 3500 K, a radius of ∼230 R⊙, and a high luminosity L ∼ 7400 L⊙. The hot component is a hot white dwarf. We tentatively determined the orbital period of the system ∼487 d. The main outburst of Gaia18aen in 2018 was accompanied by a decrease in the temperature of the hot component. The first phase of the outburst was characterized by the high luminosity L ∼ 27 000 L⊙, which remained constant for about three weeks after the optical maximum, later followed by the gradual decline of luminosity and increase of temperature. Several re-brightenings have been detected on the timescales of hundreds of days.
Screening of Bufadienolides from Toad Venom Identifies Gammabufotalin as a Potential Anti-inflammatory Agent
Authors: Zheng Yuanru , Deng Lijuan , Cao Huihui , Xu Nishan , Zhang Dongmei , Tian Haiyan , Li Baojing , Lu Zibin , Ye Wencai , Yu Linzhong , Fan Chunlin , Liu Junshan 
Published: October 2020

AbstractToad venom (Chansu) is used in the treatment of infectious and inflammatory diseases in China and East/Southeast Asian countries. However, the anti-inflammatory components of toad venom have not yet been systematically evaluated and clearly defined. To investigate the anti-inflammatory effects of toad venom and identify new anti-inflammatory ingredients, we used zebrafish, an alternative drug screening model, to evaluate the anti-inflammatory effects of 14 bufadienolides previously isolated from toad venom. Most of the bufadienolides were found to exert significant anti-inflammatory effects on lipopolysaccharide-, CuSO4-, or tail transection-induced zebrafish inflammatory models. Moreover, gammabufotalin (6) inhibits lipopolysaccharide-induced inflammation by suppressing the myeloid differentiation primary response 88/nuclear factor-kappa B and STAT3 signal pathways. This study confirms the potential of zebrafish in drug screening, clarifies the anti-inflammatory effects of bufadienolides from toad venom, and indicates that gammabufotalin may be developed as a novel therapeutic agent for inflammatory diseases in the future.
Importance and Evidence of Extent of Resection in Glioblastoma
Authors: Wykes Victoria , Zisakis Athanasios , Irimia Mihaela , Ughratdar Ismail , Sawlani Vijay , Watts Colin 
Published: October 2020

AbstractMaximal safe resection is an essential part of the multidisciplinary care of patients with glioblastoma. A growing body of data shows that gross total resection is an independent prognostic factor associated with improved clinical outcome. The relationship between extent of glioblastoma (GB) resection and clinical benefit depends critically on the balance between cytoreduction and avoiding neurologic morbidity. The definition of the extent of tumor resection, how this is best measured pre- and postoperatively, and its relation to volume of residual tumor is still discussed. We review the literature supporting extent of resection in GB, highlighting the importance of a standardized definition and measurement of extent of resection to allow greater collaboration in research projects and trials. Recent developments in neurosurgical techniques and technologies focused on maximizing extent of resection and safety are discussed.