bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in physical activity, sedentary behaviour and body composition: longitudinal analysis in the PREDIMED-Plus trial
Authors: Galmes-Panades Aina M , Konieczna Jadwiga , Varela-Mato Veronica , Abete Itziar , Babio Nancy , Fiol Miquel , Paz Jose Antonio de , Casas Rosa , Olbeyra Romina , Ruiz-Canela Miguel , Galindo Antoni Palau , Castañer Olga , Martín-García Arturo , Estruch Ramon , Vidal Josep , Buil-Cosiales Pilar , Wärnberg Julia , Salas-Salvado Jordi , Martinez J Alfredo , Romaguera Dora 
Published: July 2020

Abstract
Background:The optimal distribution between physical activity (PA) levels and sedentary behaviour (SB) for the greatest benefits for body composition among older adults with overweight/obesity and chronic health conditions remains unclear. We aimed to determine the prospective association between changes in PA and in SB with concurrent changes in body composition. To examine whether reallocating inactive time into different physical activity levels was associated with 12-month change to body composition in older adults.Methods: A subsample (n=1564) of men and women (age: 55-75 years) with overweight/obesity and metabolic syndrome from the PREDIMED-Plus trial was included in the present analysis. Participant’s were followed-up at 6- and 12-months. Physical Activity and SB were assessed using validated questionnaires. Out of 1564 participants, 388 wore an accelerometer to objectively measure inactive time and PA over a 7-day period. At each time point, participants body composition was measured using dual-energy X-ray absorptiometry (DXA). Standard covariate-adjusted and isotemporal substitution modelling were applied to linear mixed-effects models.Results:Increasing 30 minute of total PA and MVPA were associated with significant reductions in body fat (β -0.07% and -0.08%) and VAT (-13.9g, and -15.6g) at 12-months (all p-values <0.001). Reallocating 30 minute of inactive time to MVPA was associated with reductions in body fat and VAT, and with an increase in muscle mass and muscle-to-fat mass ratio (all p-values <0.001).Conclusions:At 12 months, increasing total PA and MVPA and reducing total and TV-viewing SB were associated with improved body composition in participants with overweight or obesity, and metabolic syndrome. This was also observed when reallocating 30 minute of inactive time by total PA, LPA and MVPA, with the greatest benefits observed for MVPA.Trial registration: The trial was registered at the International Standard Randomized Controlled Trial (ISRCTN: http://www.isrctn.com/ISRCTN89898870) with number 89898870 and registration date of 24 July 2014, retrospectively registered.
Molecular and clinical characterization of CCT2 expression at transcriptional level via 2994 samples of breast cancer
Authors: Liu Qiang , Qi Yihang , Wang Xiangyu , Kong Xiangyi , Fang Yi , Wang Jing 
Published: July 2020

Abstract
BackgroundMolecular chaperones play important roles in regulating various cellular processes and malignant transformation. Expression of some subunits of molecular chaperonen CCT/TRiC complex have been reported to be correlated with cancer development and patient survival. However, little is known about the expression and prognostic significance of Chaperonin Containing TCP1 Subunit 2 (CCT2), a gene encoding a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC).MethodThrough the Cancer Genome Atlas (TCGA) and Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) databases, we systematically reviewed a total of 2994 cases with transcriptome data and analyzed the functional annotation of CCT2 by Gene ontology (GO) and KEGG analysis. Univariate and multivariate survival analysis were performed to investigate the prognostic value of CCT2 in breast cancer.ResultsWe found CCT2 was significantly upregulated in various tumors. In breast cancer, CCT2 expression was significantly upregulated in HER2-positive (HER2+) group, and more malignant group. In addition, we investigated correlations between CCT2 and other CCT members. Interestingly, almost all CCTs expression were positively correlated with each other, but not CCT6B. Survival analysis suggested that CCT2 overexpression was independently associated with worse prognosis of patients with breast cancer, especially in luminal A subtype.ConclusionIn summary, our results revealed that CCT2 might be involved in regulating cell cycle pathway, and independently predicted worse prognosis in breast cancer patients. These findings may expand understanding of potential anti-CCT2 treatments. To our knowledge, this is the largest and most comprehensive study characterizing the expression pattern of CCT2 together with its prognostic values in breast cancer.
Psychological factors affecting the association between a lifestyle behavior intervention and depressive symptoms in low-income overweight and obese mothers with young children: a secondary data analysis
Authors: Robbins Lorraine B. , Chang Mei-Wei , Ling Jiying , Brown Roger 
Published: July 2020

Abstract
Background Psychological factors mediating the effect of lifestyle behavior interventions on mothers’ depressive symptoms are unclear. Using data from a community-based lifestyle behavior intervention, this secondary analysis examined whether autonomous motivation, coping self-efficacy, and emotional coping mediated the association between the intervention and depressive symptoms in low-income overweight and obese mothers with young children.Methods This 16-week intervention had 2 components: Mothers watched 10 video lessons in Digital Video Disc (DVD) format at home and joined 10 peer support group teleconferences (every week in weeks 1–4; then every other week in weeks 5–16). All participants completed reliable and valid measures of autonomous motivation, coping self-efficacy, emotional coping and depressive symptoms via phone at baseline and immediately after the intervention. To assess effect size, proportion of maximum possible (POMP) scores were calculated. To test mediation effects, composite indicator structural equation modeling was performed.Results The intervention significantly alleviated depressive symptoms (p = 0.015, POMP = -4.31%). While the intervention had no influence on autonomous motivation, it significantly increased coping self-efficacy (p < 0.001, POMP = 7.4%) and emotional coping (p = 0.003, POMP = 3.81%). Coping self-efficacy significantly and negatively mediated the association between the intervention and depressive symptoms (p = 0.002, POMP = -2.53%). However, autonomous motivation and emotional coping were not significant mediators.Conclusions To help this target population alleviate depressive symptoms, clinicians may consider teaching these women practical strategies that can be applied to daily life to boost coping self-efficacy.
Development And Validation Of Models To Predict Cesarean Delivery Among Low-Risk Nulliparous Women At Term: A Retrospective Study In China
Authors: Sun Fangcan , Han Bing , Wu Fangfang , Shen Qianqian , Shen Minhong , Chen Youguo 
Published: July 2020

Abstract
Background: Cesarean delivery after failure of trial of labor is associated with adverse maternal and perinatal outcomes. A prediction algorithm to identify women with high risk of an emergency cesarean could help reduce morbidity and mortality associated with labor. The objective of the present study was to derive and validate a simple model to predict cesarean delivery for low-risk nulliparous women in Chinese population.Methods: This retrospective study analyzed the low-risk nulliparous women with singleton cephalic full-term fetus delivered in two medical centers. After the clinical data of the women who delivered at the tertiary referral center (n=6 551) was collected and was used univariate and multivariable logistic regression analysis, the prediction model was fitted. We performed external validation using data from nulliparous who delivered from another hospital(secondary referral center, n=7 657). A new nomogram was established based on the development cohort to predict the cesarean. The ROC curve, calibration plot and decision curve analysis were used to assess the predictive performance. Results: The cesarean delivery rates in the development cohort and the external validation cohort were 8.79% (576/6 551) and 7.82% (599/7 657). Multivariable logistic regression analysis showed that maternal age, height, BMI, weight gained during pregnancy, gestational age, induction method, meconium-stained amniotic fluid and neonatal sex were independent factors affecting cesarean outcome. Because sex of the fetuses were unknown until they born(China's Fertility Policy), we established two prediction models according to fetal sex was involved or not. The AUC was 0.782 and 0.774, respectively. The Hosmer-Lemeshow goodness-of-fit test showed that these two models fitted well. Decision curve analysis demonstrated that the models were clinically useful. And internal validation using Bootstrap method showed that these prediction models perform well. On the external validation set, the AUC were 0.775 and 0.775, respectively. The calibration plots for the probability of cesarean showed a good correlation. The online web server was constructed based on the nomogram for convenient clinical use.Conclusions: Both two models established by these factors have good prediction efficiency and high accuracy, which can provide the reference for clinicians to guide pregnant women to choose an appropriate delivery mode.
Prevalence of Coronavirus Disease 2019 in Rheumatic Patients and Evaluation of The Effect of Disease-Modifying Anti-Rheumatic Drugs
Authors: Moradi Soroush , Mohammadi Somayeh , Vafaeimanesh Jamshid , Mohseni Mohaddeseh , Mahdavi Hossein , Aryannejad Armin , Masoumi Maryam 
Published: July 2020

Abstract
Background: One of the most controversial issues among rheumatologists is the best approach to managing a rheumatic patient (RP) with coronavirus disease 2019 (COVID-19).Objectives: This study aims to evaluate the prevalence of COVID-19 in RPs and compare it to thegeneral population (healthy individuals). Besides, it assesses the incidence of COVID-19 based on rheumatologic diseases (RDs) categories and immunosuppressive (IS) drug history.Methods: In a cross-sectional study, all RPs of the rheumatology clinic of Shahid BeheshtiHospital, Qom, Iran were included (the case group), and the prevalence of COVID-19 was compared to that in healthy individuals (the control group), between December 1, 2019, andFebruary 29, 2020. Qom city was the first city in Iran in which COVID-19 was identified and spread rapidly. The participants were recruited from hospital records for the case group and QomHealth Network’s database for the control group.Results: The prevalence of COVID-19 is significantly less in RPs than in the healthy population.Moreover, patients who were under treatment with biologic diseas-modifying anti-rheumatic drugs(DMARDs) and IS drugs had milder symptoms in the case of COVID-19. Two RPs died from COVID-19, both of whom had granulomatosis and polyangiitis (GPA).Conclusion: The prevalence of COVID-19 in the RPs was lower than the general population, which was determined to be associated with more adherence to the quarantine and social distancing rules by RPs. Besides, using leflunomide (a DMARD) and IS drugs might have a protective effect against severe COVID-19.
Protein over-expression in Escherichia coli triggers adaptation analogous to antimicrobial resistance
Authors: James Jack , Yarnall Benjamin , Koranteng Andy , Gibson Jane , Rahman Tahmina , Doyle Declan Anthony 
Published: July 2020

Abstract
Background
The E. coli pET system is the most widely used protein over-expression system worldwide. It relies on the assumption that all cells produce target protein and it is generally believed that integral membrane protein (IMP) over-expression is more toxic than their soluble counterparts.
Results
Using GFP-tagged proteins, high level over-expression of either soluble or IMP targets results in > 99.9% cell loss with survival rate of only < 0.03%. Selective pressure generates three phenotypes: large green, large white and small colony variants. As a result, in overnight cultures, ~ 50% of the overall cell mass produces no protein. Genome sequencing of the phenotypes revealed genomic mutations that causes either the loss of T7 RNAP activity or its transcriptional downregulation. The over-expression process is bactericidal and is observed for both soluble and membrane proteins.
Conclusions
We demonstrate that it is the act of high-level over-expression of all exogenous proteins in E. coli that sets in motion a chain of events leading to > 99.9% cell death. These results redefine our understanding of protein over-production and link it to the adaptive survival response seen in the development of antimicrobial resistance.
Validation of 4 Estimating Methods to Evaluate 24-hour Urinary Sodium Excretion for Young Adults in China by Different Seasons
Authors: Gong Weiyi , Zhang Zechen , Liang Jufeng , Zhang Jiguo , Ma Yuxia 
Published: July 2020

Abstract
Background: 24-hour urine sample collection is regarded as the gold standard for sodium intake evaluation, but the implementation can be difficult. To validate and evaluate the accuracy and feasibility of estimating sodium intake by four methods of Kawasaki, INTERSALT, Tanaka and Sun’s equations. Methods: 274 healthy volunteers aged 18-25y were enrolled in this study. 24-hour urine samples as well as timed (morning, afternoon, evening and overnight) urine samples were randomly collected. The sodium intake were estimated by 4 published equations—Kawasaki, INTERSALT Tanaka and Sun’s. The consistencies between estimated sodium intake and real measured values of 24-hour urine sodium excretion were compared by Bland-Altman plots in each of the methods. Taking the variability of sodium excretion in different seasons into account, this study was conducted separately in summer (end of June 2017) and fall (end of November 2017). Results: The 24-h urinary sodium analysis result indicated an average daily sodium intake of 3043.0±1223.3mg in summer, and 3563.7±1370.0mg in the fall. Compared with autumn, the average daily reduction in sodium was about 520.7 mg. From the group level, the mean bias (estimated value - measured value) of the INTERSALT method with morning specimens (-39.7 mg ) was the smallest in summer. The mean bias of Tanaka method for prediction of morning specimens (-149.8 mg ) was the smallest in autumn. For the individual level, the correlation between 24-hour urinary sodium excretion and Sun’s methods was low, with a correlation coefficient of 0.406 in summer and 0.476 in the fall. Correlation coefficients between Kawasaki, INTERSALT, Tanaka methods and 24-hour urinary sodium excretion, respectively was in the range of 0.48-0.64. The correlation coefficient of Kawasaki method was the highest in summer (0.612) while that of Tanaka method with the afternoon specimens was the highest in the autumn (0.635). Conclusions: Estimation of sodium intakes among young adults using the 4 methods have varying degrees of bias and volatility in different times and seasons.
Expression of Catechol O methyl transferase (COMT) with the contrasting effects of 17β estradiol (E2): Implications for the mechanism of estrogen-induced ovarian carcinogenesis.
Authors: El-Etreby Nour Mohamed , Osman Eman Medhat , Badawy Nehal Abd El Latif El , Nour Hoda Abd El Hamid 
Published: July 2020

Abstract
Background
Epidemiological data show that induction of ovarian cancer is related to estrogen exposure and metabolism. In addition catechol metabolites of estrogen also contribute to carcinogenesis. O methylation by Catechol O methyl transferase (COMT) is a phase II metabolic inactivation pathway for catechol estrogens. The goal of the present study was to investigate the role of COMT level in ovarian carcinogenesis with the contrasting effects of 17 β estradiol level.
Subjects and methods
Our study was conducted on 80 subjects divided into 30 patients with malignant ovarian tumors ,30 patients with benign ovarian tumors and 20 healthy controls. Tissue and serum levels of COMT and 17 17 β estradiol were determined using ELISA
Results
According to our results COMT inhibition in the malignant group was detected as high as 7.1 pmol/L E2 in serum and 15.6 pmol/L E2 in tissue homogenate. This inhibition was absent in the benign group as high as 7.53 pmol/L E2 in serum and as high as 14.9 pmol/L E2 in tissue homogenates.
Conclusions
Our results provide evidence for the protective effect of COMT in benign ovaries against neoplastic transformation. This supports the notion that targeting the metabolism of estrogen can be an another way to reduce ovarian cancer risk.
Study on the Characteristics of Gut Microbiota in Chronic Hepatitis B Patients with Damp Heat Syndrome and Liver Depression and Spleen Deficiency Syndrome
Authors: Jiang Kaiping , Jiang Qunfang , Mo Xiaoai , Li Jianhong , Hu Hongtao , Huang Qinghua , Guo Wenqiang , Qiu Tengyu , Ren Jian , Zhang Lei , Xie Youqing , Huang Kaizhou 
Published: July 2020

Abstract
Background: To explore the potential biological characteristics of traditional Chinese medicine(TCM) syndromes in CHB patients is of great significance to improve the level of TCM treatment.This study was designed to explore the characteristics of gut microbiota in CHB patients with two most common TCM Syndromes.Methods: According to the diagnostic criteria of Western medicine, TCM and screening exclusion criteria, 65 cases of CHB patients with damp heat syndrome and 28 cases of CHB patients with liver depression and spleen deficiency syndrome were finally included in the study. All the basic information was gathered and the fresh fecal samples were collected for 16S rDNA sequencing. 16S rDNA of gut microbiota was sequenced using Illumina hiseq 2500 high-throughput sequencing platform.Based on the optimized sequence, OTU clustering analysis and taxonomic annotation were carried out. Results: The difference in relative abundance of gut microbiota was significant between damp heat syndrome and liver depression and spleen deficiency syndrome in CHB patients. Cyanobacteria was only found in damp heat syndrome.The relative abundance of Erysipelotrichia and Subdoligranulum were higher in liver depression and spleen deficiency syndrome,while the relative abundance of Rhodospirillales, Alphaproteobacteria,and Lachnospira were higher in the damp heat syndrome.LEfSe analysis showed that Lachnospira,Olsenella and Subdoligranulum had significant difference in species among the two TCM syndromes.Conclusions: The different characteristics of gut microbiota in the two TCM syndromes of CHB patients may play an important role in syndrome formation of TCM,which provides a new field of vision for the accurate diagnosis and treatment of TCM.
Upregulation of RBM24 Exacerbates Bladder Cancer Progression by Formatting Runx1t1/TCF4/miR-625-5p Feedback Loop
Authors: Yin Yue-Wei , Liu Kai-Long , Lu Bao-Sai , Li Wei , Niu Ya-Lin , Zhao Chen-Ming , Yang Zhan , Guo Ping-Ying , Qi Jin-Chun 
Published: July 2020

Abstract
Background: RNA-binding motif protein 24 (RBM24) acts as a multifunctional determinant of cell fate, proliferation, apoptosis, and differentiation during development through regulation of pre-mRNA splicing and mRNA stability. It is also implicated in carcinogenesis, but the functions of RBM24 in bladder cancer (BC) remains unclear.Methods: Cell viability was examined by colony forming and MTT assays. Real-time quantitative PCR (RT-qPCR) and western blot analysis were used to detect the protein and mRNA levels. Co-immunoprecipitation (CoIP) and proximity ligation assay (PLA) were used to determine the protein-protein interaction. Chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP), and oligo pull-down assays were used to verify DNA/RNA–protein interactions. Luciferase assay analysis was used to detect effects on transcription factor activity.Results: In the present study, we revealed that RBM24 was upregulated in BC tissues. Importantly, we found that higher level of RBM24 was correlated with poor prognosis in BC patients. Overexpression of RBM24 promoted while depletion of RBM24 inhibited BC cell proliferation in vivo and in vitro. Mechanically, RBM24 positively regulated Runx1t1 expression in BC cells by binding to and enhancing Runx1t1 mRNA stability. Runx1t1 in turn promoted RBM24 expression by interacting with TCF4. Furthermore, Runx1t1 in turn promoted RBM24 expression by interacting with the transcription factor TCF4 and depressing transcription of miR-625-5p, which directly targets and normally suppresses RBM24 expression. RBM24-regulated BC cells proliferation was moderated via the Runx1t1/TCF4/miR-625-5p feedback loop.Conclusions: In summary, these results indicate that a RBM24/Runx1t1/TCF4/miR-625-5p positive feedback loop plays a key role in BC oncogenesis. Disruption of this pathway may be a potential therapeutic strategy for BC treatment.
Expression of Cancer testis Antigens in Tumor-adjacent Normal Liver Predicts Post-resection Recurrence of Hepatocellular Carcinoma
Authors: Noordam Lisanne , Ge Zhouhong , Özturk Hadiye , Doukas Michail , Mancham Shanta , Boor Patrick P.C. , Carrascosa Lucia Campos , Zhou Guoying , Bosch Thierry P.P. van den , Pan Qiuwei , IJzermans Jan N.M. , Bruno Marco J. , Sprengers Dave , Kwekkeboom Jaap 
Published: July 2020

Abstract
Background: High recurrence rates after resection of hepatocellular cancer (HCC) with curative intent and lack of effective therapy for advanced disease impair clinical outcomes of HCC. Cancer/testis antigens (CTAs) are suitable targets for cancer immunotherapy if selectively expressed in tumor cells. The aims of this study were to establish a panel of CTAs that are frequently and selectively expressed in tumors of HCC-patients, and to investigate whether CTAs might be expressed in tumor-free liver tissues of HCC-patients.Methods: Surgically-resected tumor and paired tumor-free (TFL) tissues of HCC patients (n=100), healthy livers (n=21), and other healthy tissues (n=22 different tissues) were assessed for mRNA expression of 49 carefully selected CTAs by RT-qPCR. Protein expression of 5 CTAs was determined by immunohistochemistry (n=78).Results: Twelve CTAs were expressed at mRNA level in ≥10% of HCC-tumor tissues and not in healthy tissues except testis. In tumors, mRNA and protein of ≥ 1 CTA was expressed in 78% and 71% of HCC-patients, respectively. In TFL, CTA mRNA and protein expression was found in 45% and 30% of HCC-patients, respectively. Interestingly, CTA expression in TFL was an independent negative prognostic factor for HCC-recurrence and survival after tumor resection.Conclusions: We established a novel panel of 12 testis-restricted CTAs expressed in tumors of most HCC-patients, that can be safely used for immunotherapeutic targeting of HCC. The increased risk of HCC recurrence in patients with CTA expression in TFL suggests that CTA-expressing (pre-)malignant cells may be a source of HCC recurrence. Therefore, immunotherapeutic targeting of these antigens should be considered as adjuvant therapy to prevent HCC-recurrence after tumor resection. Lay summary:Expression of multiple defined cancer testis antigens in non-cancerous liver tissue is associated with significantly increased cancer-recurrence and worse patient survival after tumor resection. We propose that immunotherapeutic targeting of these antigens may prevent HCC recurrence after tumor resection.