bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acupuncture Collaborative Care for Patients with Poststroke Cognitive Impairment: Protocol for a Randomized Controlled Trial
Authors: Liu Ruijia , Yu Xudong , Wang Jisheng , Liu Ye , Liu Bowen , Li Xinwei , Wang Yue , Cao Tianyu , Yuan Hongwei 
Published: March 2020

Abstract
Background: PSCI is a series of syndromes that meet the diagnostic criteria for cognitive impairment that appear after a stroke.The treatment of PSCI with oral drugs alone is not ideal and has obvious side effects. Therefore, complementary and alternative treatments are needed for patients with insufficient or significant side effects of oral medications.Therefore, we will evaluate the clinical effectiveness and safety of acupuncture in the treatment of PSCI.Methods/design:In this study, patients will be randomly divided into two groups. Intervention group: Acupuncture combined with oral medication. Control group: Western medicine treatment plan.All participants will continue to receive conventional drug treatment. The selection of outcomes will be evaluated by Mini Mental State Examination Scale (MMSE) at week 12.Discussion:This trial may provide evidence regarding the clinical effectiveness, safety, and cost-effectiveness of acupuncture for patients with PSCI.Trial registration: Trial registration:ClinicalTrials.gov,ChiCTR2000029926, Registered on 17 February 2020，http://www.chictr.org.cn/showproj.aspx?proj=49356
Impact of fecal microbiota transplantation on chronic recurrent pouchitis in ulcerative colitis with ileo-anal anastomosis: study protocol for a prospective, multicenter, double-blind, randomized, controlled trial
Authors: Trang-Poisson Caroline , Kerdreux Elise , Poinas Alexandra , Planche Lucie , Sokol Harry , Bemer Pascale , Cabanas Karine , Hivernaud Eliane , Biron Laetitia , Flet Laurent , Montassier Emmanuel , Garcasson Ghislaine Le , Chiffoleau Anne , Jobert Alexandra , Lepelletier Didier , Caillon Jocelyne , Pape Patrice Le , Imbert Berthe-Marie , Bourreille Arnaud 
Published: March 2020

Abstract
- Background: Almost 15 % of patients with ulcerative colitis (UC) will require a proctocolectomy with ileal pouch–anal anastomosis (IPAA) as a result of fulminant colitis, dysplasia, cancer, or medical refractory diseases. Around 50% will experience pouchitis, an idiopathic inflammatory condition involving the ileal reservoir, responsible for digestive symptoms, deterioration in quality of life, and disability. Though the majority of initial cases of pouchitis are easily managed with a short course of antibiotics, in about 10% of cases, inflammation of the pouch becomes chronic with very few treatments available. Previous studies have suggested that manipulating the composition of intestinal flora through antibiotics, probiotics, and prebiotics achieved significant results for treating acute episodes of UC-associated pouchitis. However, currently there is no established effective treatment for chronic antibiotic-dependent pouchitis. Fecal microbiota transplantation (FMT) is a novel therapy involving the transfer of normal intestinal flora from a healthy donor to a patient with a medical condition potentially caused by the disrupted homeostasis of intestinal microbiota or dysbiosis.-Methods Our project aims to compare the delay of relapse of chronic recurrent pouchitis after FMT versus sham transplantation. Forty-two patients with active recurrent pouchitis after having undergone an IPAA for UC will be enrolled at 12 French centers. The patients who respond to antibiotherapy will be randomized at a ratio of 1:1 to receive either FMT or sham transplantation.-Discussion : On April 30, 2014, WHO published an alarming report on antibiotic resistance. Finding an alternative medical treatment to antibiotics in order to prevent relapses of pouchitis is therefore becoming increasingly important given the risk posed by multiresistant bacteria. Moreover, if the results of this study are conclusive, FMT, which is less expensive than biologics, could become a routine treatment in the future.-Trial registration: The trial, published on May 14, 2018, is registered at ClinicalTrials.Gov under registration number NCT03524352: https://clinicaltrials.gov/ct2/show/NCT03524352term=NCT03524352&rank=1
The Prevalence And Associated Factors Of Alcohol Use Among Pregnant Women Attending Antenatal Care At Public Hospitals Addis Ababa, Ethiopia, 2019.
Authors: Tadesse Getaneh Tesfaye , Demlew Demeke , G/tsadik Meseret , Habte Fikreselam , Kassie Gebeyaw Molla , Kifle Yohannes , Gebreegziabher Gebreslassie 
Published: March 2020

Abstract
Background

Alcohol use during pregnancy is a significant public health problem, ultimately affecting the neonatal offspring. Recent studies explore that no safe amount and safe time to drink alcohol during pregnancy. Even though drinking in pregnancy has a wide range of problems, a small number of scientific publications document on the magnitude of drinking alcohol during pregnancy in Sub-Saharan African countries including Ethiopia. The aim of this study was to assess prevalence and associated factors of alcohol use among pregnant women attending prenatal care at public hospitals, Addis Ababa, Ethiopia.

Methods

An Institution based cross sectional study was employed from May 7 to June 6, 2019 at public hospitals, Addis Ababa. A total of 585 pregnant women participated in the study selected through systematic random sampling technique. Data was collected using interviewer administered structured and semi structured questionnaires. Frequency of consumption was measured by using AUDIT. The collected data was entered into EPI data and then exported to SPSS version 25 for analysis. Frequency tables and graphs were used to describe the study variable. The association between variables analyzed with bi-variable and multivariable binary logistic regression. A statistical significance was declared at p value < 0.05 with 95% confidence interval

Result

A total of 585 participants were included in the study with the response rate of 98.6%. The study showed that the prevalence of alcohol use among pregnant women were 37.1% with (95% CI, 33.2 – 41). Factors like no formal education [AOR= 3.22, 95%CI, 1.72, 6.02], pre-pregnancy alcohol use [AOR= 3.16, 95%CI, 2.03, 4.91], partner alcohol use [AOR= 3.43, 95%CI, 2.21, 5.32], and poor social support [AOR= 3.16, 95%CI, 1.88, 5.31] were statistically associated with alcohol use during pregnancy.

Conclusion

In this study the prevalence of alcohol use during pregnancy was high as compared to majority of other studies. This study observed that no formal education, pre-pregnancy alcohol use, partner alcohol use, and poor social support, were statistically significant with alcohol use during pregnancy. Based on the findings of this study early management of alcohol use and problematic alcohol use is needed for pregnant women.
Serum uromodulin is a novel predictive marker of renal function in the Japanese population
Authors: Usui Ryosuke , Ogawa Tetsuya , Takahashi Hideo , Iwasaki Chihiro , Koike Minako , Morito Taku , Hatano Michiyasu , Nitta Kosaku 
Published: March 2020

Abstract
Background Uromodulin, also known as Tamm-Horsfall protein, is the most abundant protein in urine. It has recently been reported that uromodulin exists in a small amount in blood and that its concentration correlates with the estimated glomerular filtration rate (eGFR). However, its clinical significance has not been clarified and there are no relevant data for the Japanese population. Method First, we generated anti-human uromodulin mouse monoclonal antibodies and established a specific enzyme-linked immunosorbent assay for uromodulin. We then performed an observational clinical study to determine if there was a correlation between serum uromodulin concentration and estimates of kidney function and whether the serum uromodulin value could be a biomarker in clinical nephrology. The clinical study included 308 patients with and without chronic kidney disease and healthy volunteers. Serum concentrations of creatinine, cystatin C, and uromodulin were measured and correlations were sought between the eGFR calculated from the creatinine and cystatin C levels and the serum uromodulin concentration. Results There was a good correlation between the serum uromodulin concentration and the eGFR value calculated from the creatinine (r=0.76) and cystatin C (r=0.79) levels. The mean serum uromodulin level in the group with an eGFR >90 mL/min/1.73 m 2 calculated using cystatin C was significantly higher than that in the group with an eGFR of 80–89 mL/min/1.73 m 2 . Conclusions The serum uromodulin measurement could be a useful biomarker for identification of patients with early deterioration of kidney function.
N-acetylcysteine resists high-glucose-induced injury of human umbilical vein endothelial cells by inhibiting the leptin/leptin receptor
Authors: Wang Zhenhua , Chen Jun , Lian Shuping , Liao Kaibin , Huang Lizhi , Li Chaosheng 
Published: March 2020

Abstract
Baclground The present study aimed to investigate whether N- acetylcysteine (NAC) protects human umbilical vein endothelial cells (HUVECs) against high glucose (HG)-induced injury by inhibiting leptin/leptin receptor (LEPR).Methods HUVECs were treated with 40 mmol/L glucose for 24 h to establish a model of HG-induced endothelial cell injury; The cell viability was examined by cell counter kit-8(CCK-8) assay; The expression levels of leptin, LEPR, cleaved caspase-3 and endothelial nitric oxide synthase (eNOS) were detected by western blot. The intracellular levels of reactive oxygen species (ROS) were tested by DCFH-DA staining followed by photofluorography. Tumor necrosis factor-α (TNF-α)、nuclear factor-kappa B (NF-κB) and intercellular adhesion molecule-1 (ICAM-1) were detected by enzyme-linked immunosorbent assay (ELISA). The number of apoptotic cells was observed by photofluorograph with Hoechst 33258 nuclear staining. Mitochondrial membrane potential (MMP) was obtained using JC-1. Results The expression of leptin and LEPR began to significantly increase after exposure to 40 mmol/L HG for 24 h. Pretreatment of HUVECs with 7 mmol/L NAC or 50 ng/mL leptin antagonists (LA) for 30min inhibited the increased expression of leptin and LEPR induced by HG in HUVECs. Furthermore, pretreatment with 7 mmol/L NAC or 50 ng/mL LA for 30 min also inhibited HG-induced injury, by increasing the cell viability and eNOS expression, and decreasing the inflammatory response and cleaved caspase-3 expression, the apoptotic cells and generation of intracellular ROS and a loss of MMP. Conclusions NAC protects the HUVECs against HG-induced injury by inhibiting leptin/LEPR.
Hypertrophic pachymeningitis in a southern Chinese population: a retrospective study
Authors: Xiao Xuewen , Fu Dongni , Feng Li 
Published: March 2020

Abstract
Background Hypertrophic pachymeningitis (HP) is a fibrotic disorder featuring a thickening of the dura matter. Most HP studies were from Caucasian population and only a few studies of HP are available in China. In this study, we investigated the causes, clinical and imaging features and therapeutic implications of HP in a southern Chinese population.Methods We retrospectively analyzed 48 patients with HP with different causes from 1 January 2006 to 31 December 2018. Clinical manifestation, laboratory findings, neuroimaging results, and clinical course were evaluated in all HP patients.Results The mean age at onset was 50±12 years. The most common diagnosis was idiopathic HP (67%), followed by ANCA-associated vasculitis (15%), tuberculous meningitis (8%), viral meningitis (6%), and bacterial meningitis (4%). The main clinical manifestations were headache and cranial nerve deficits. The most frequently changed laboratory finding was elevated ESR. Imaging was characterized by cerebral or spinal dura matter enhancement in MRI scan with contrast. Enhancements were mainly located in the posterior fossa for idiopathic HP; frontal, parietal and occipital lobes for ANCA-related HP; and the posterior fossa for tuberculous-associated HP. Diffuse enhancement was found in most cases, except for tuberculous-associated HP. Glucocorticoid or immunosuppressive treatment were applied in most cases.Conclusions HP was diagnosed based on clinical manifestations, imaging and laboratory results. Etiology varied among patients, with idiopathic HP being the most common. MRI with contrast showed enhancement of the dura matter, which differed according to different etiologies. Glucocorticoid or immunosuppressive agents were the primary drugs for treatment.
From Great Genetics To Neuropsychology – Outline Of The Research On The Association Between Microbiota And Human Behaviour
Authors: Czajeczny Dominik , Kabzińska Karolina , Wójciak Rafał Wojciech 
Published: March 2020

Abstract
After the success of the Human Genome Project, which lead to estimating the number of human genes at only about 30–40 thousand, researchers started paying attention to a great number of genes present inside and on the surface of the human body. The gastrointestinal tract alone is a habitat for up to 1000 species of bacteria and other microorganisms. These microorganisms add a pool of 2–4 million additional genes. In 2009, a hypothesis was proposed that changes in microbiota are sensed by neurons localized along the entire bowel length, and communicated to the brain, making up the gut-brain axis. The vagus nerve seems to serve as the main communication path. Besides affecting gastrointestinal tract functions, primary neuropsychological studies show that gut microbiota is linked to HPA activity, and thus with arousal regulation and emotional functions. Research also suggests a link to cognitive functions. For now, mechanisms of those connections remain, for the most part, unknown. History of the research on human microbiota shows a complex nature of human functions and the need for integration of knowledge from, as it may seem, distant branches of science, like microbiology and psychology. While connections between microbiota and host organism remain unrecognized, our knowledge of human biology will be incomplete.
1. Human Genome Project. 2. In search for the missing genes. 3. Human Microbiome Project. 4. In search for the structure in infinity. 5. Gut-brain axis, towards neuropsychology. 6. Conclusions
Clinically Used And Potential Antimycotics In The Context Of Therapy Of Dermatomycoses
Authors: Gnat Sebastian , Łagowski Dominik , Nowakiewicz Aneta , Dyląg Mariusz 
Published: March 2020

Abstract
Paradoxically, despite the progress in medicine, the prevalence of fungal infections is increasing from year to year. At the beginning of the third millennium, practical therapeutic options are still very limited. Currently, only eight classes of antifungal compounds are in clinical use, only four of which are used in the treatment of dermatomycoses. The intense search for the “Holy Grail” of antifungal therapy that has been going on since the second half of the 20th century faces serious obstacles arising from the eukaryotic model of fungal cell structure. In this paper, new groups of chemical compounds of mainly natural origin have been synthetically described, which due to their interesting antifungal activity, including pathogenic species of dermatophytes, may constitute new therapeutic options. Among compounds currently arousing great interest, compounds from the group of terpenoids, alkaloids, saponins, flavonoids and essential oils deserve attention. Many of these compounds are in clinical trials as potential antifungal agents, while others are in preclinical studies. Future research should focus on attempting to determine the applicability of the given substances in implementation for routine use and their effectiveness, toxicity and side effects.
1. Introduction. 2. General characteristics of dermatophytes in the therapeutic aspect. 3. New synthetic preparations with antifungal activity. 4. Natural antifungal preparations. 4.1. Terpenoids and essential oils. 4.2. Alkaloids. 4.3. Flavonoids. 4.4. Saponins. 4.5. Other chemical compounds 5. Summary
Biotechnological And Biomedical Applications Of Fungal Cellobiose Dehydrogenase
Authors: Olszewska Katarzyna , Olszewska Anna , Rogalski Jerzy , Sulej Justyna 
Published: March 2020

Abstract
Cellobiose dehydrogenase (CDH) is an extracellular oxidoreductive enzyme produced by wood-decaying fungi belonging to the phylum Basidiomycota and Ascomycota. This enzyme has a binary structure containing two cofactors (FAD and hem), located in separate domains and connected by a proteolytically sensitive linker. Due to its unique structure and properties, CDH has great potential for application in both biotechnology and biomedical applications. The aim of this paper is to review the literature on catalytic properties of cellobiose dehydrogenase and its potential applications.
1. Introduction. 2. Cellobiose dehydrogenase. 2.1. History of discovery and classification of the enzyme. 2.2. Structure, mechanism of action and properties. 3. Application potential of cellobiose dehydrogenase. 3.1. Biomedical applications. 3.2. Application of cellobiose dehydrogenase in biotechnological processes. 4. Summary
Bionanocellulose – Properties, Acquisition And Perspectives Of Application In The Food Industry
Authors: Olędzki Remigiusz , Walaszczyk Ewa 
Published: March 2020

Abstract
The food industry is one area of industrial activities that requires the frequent implementation of technological and product innovations. Foodstuffs obtained both in technologically advanced factories, as well as in small manufacturing enterprises, are increasingly produced using innovative food additives, which include natural polysaccharide ingredients. One of these substances is bionanocellulose – microbially produced cellulose (most commonly by the genus Komagataeibacter xylinus, formerly known as Gluconacetobacter xylinus). Bionanocellulose is a polymer with exceptionally valuable functional properties resulting from its unique molecular structure (formed by the chemically ultra-pure β-1,4-glucan). The main features of bionanocellulose are high hygroscopicity, flexibility and mechanical strength. Various physical and chemical forms of bionanocellulose (produced both during surface and submerged cultivation) are increasingly used in the production of food products. The need to produce highly diversified (e.g., usable or sensory) food products as well as the increasing difficulties associated with access to conventional sources of external coal, necessitate the search of alternative culture media for the production of bionanocellulose. The aim of the work is to describe the use of alternative carbon sources for the microbiological synthesis of bionanocellulose and its application in the food industry.
Introduction. 2. Structure and physico-mechanical characteristics of bionanocellulose. 3. The process of synthesis of bionanocellulose and its importance for microorganisms. 4. Microorganisms used for the production of bionanocellulose. 5. Raw materials used in the synthesis of bionanocellulose. 6. Techniques of culturing microorganisms that produce bionanocellulose. 7. Possible applications of bionanocellulose in the food industry. 8. Conclusions
Spatial priority conservation areas for water yield ecosystem service under climate changes in Teshio watershed, northernmost Japan
Authors: Fan Min , Shibata Hideaki , Chen Li 
Published: March 2020

Abstract
Effective information regarding water yield response to climate change provides useful support for decision making in water resources management. By integrating a hydrology model into a systematic conservation model, we developed an approach for modeling impacts of climate change on the water cycles and constructing spatial priority conservation areas for water yield ecosystem services in Teshio watershed located in northernmost Japan. The climate changes were projected to have impacts in increasing surface runoff, lateral flow, groundwater discharge and water yield. Surface runoff especially decreased in April and May and increased in March and September with rising temperature. We then investigated the spatial hotspots of water yields in typical periods (February, April and October, annual average water yield) to determine spatially priority conservation areas for water resources in terms of their different protection targets. The results also indicated that the areas of spatial optimal protection for water yields across different periods dynamically changed from spatial and temporal standpoints. The optimal priority conservation areas were concentrated in the southwest, north and southeast of Teshio watershed through comprehensively taking into account water yields in typical periods. Our results indicated that combination of hydrology and systematic conservation models would improve sustainable management of water resources across the watershed.
Evaluating Two Common Strategies for Research Participant Recruitment Into Autism Studies: Observational Study
Authors: Ahmed Kelli L , Simon Andrea R , Dempsey Jack R , Samaco Rodney C , Goin-Kochel Robin P 
Published: March 2020

BackgroundOngoing research is necessary to better understand the causes of autism spectrum disorder (ASD), the developmental outcomes for individuals diagnosed with ASD, and the efficacy of the interventions. However, it is often difficult to recruit sufficient numbers of participants for studies, and despite the prevalence of ASD (currently estimated to affect 1 in 54 children), little research has focused on how to efficiently recruit participants with ASD.ObjectiveThe aim of this study was to determine the efficacy of two different paid advertisements—social media and radio advertising—in recruiting participants for a study enrolling people with ASD and their family members by examining the number of participants enrolled, the cost per participant, and the geographic reach of each type of advertising.MethodsWe examined participant enrollment in a study following nonoverlapping paid advertisements on a popular FM radio station (aired in three cities across two states) and Facebook (six advertisements that ran in five cities across two states). The total paid investment in the radio campaign was $12,030 and that in the Facebook campaign was $2950. Following the advertising campaigns, 1391 participants in the study who were affiliated with the Houston, Texas, site received email invitations to participate in a brief survey about the ways in which they learned about the study (eg, social media, medical provider, website) and which of these were most influential in their decisions to participate; 374 (26.8%) of the participants completed this survey.ResultsSocial media advertising outperformed radio in all three parameters examined by enrolling more participants (338 vs 149), with a lower average cost per participant ($8.73 vs $80.74) and a wider geographic reach, based on a comparison of the number of zip codes within and outside of Texas for questionnaire respondents who rated social media as the most influential method of contact (n=367, χ21=5.85, P=.02). Of the 374 survey participants, 139 (37.2%) reported that they had seen the study on social media prior to enrollment, while only 9 (2.4%) said they heard about it via radio.ConclusionsOur findings suggest that advertising on social media can efficiently reach a large pool of potential participants with ASD, increasing the likelihood of meeting study enrollment goals. Researchers should consider allocating at least some portion of recruitment dollars to social media platforms as a means of quickly and inexpensively reaching out to their target populations, including for studies with in-person procedures.