bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional Connection Changes among Cerebral Regions and between Hemispheres in the Acute Ischemic Stroke Patients Treated by Scalp Acupuncture: A Rs-fMRI Study
Authors: Liu Huacong , Jiang Yijing , Wang Ningning , Chen Lanpin , Gao Jingchun , Zhang Jiping , Qu Shanshan , Liu Songyan , Liu Gang , Huang Yong , Chen Junqi 
Published: November 2020

Abstract
Objective: Resting State Functional Magnetic Resonance Imaging (Rs-fMRI) was used to explore the changes on the functional connections between cerebral hemispheres and local functional activities in patients with acute ischemic stroke dysfunction treated by International Standard Scalp Acupuncture.Methods: 30 patients of acute ischemic stroke in the middle cerebral artery supply area of the dominant hemisphere were selected and randomly divided into treatment group and control group at a ratio of 1:1 according to the random number table method, with 15 patients in each group. Patients in the control group were treated with conventional western medicine, while patients in the treatment group combined the International Standard Scalp Acupuncture (MS5, MS6 and MS7). All the patients were evaluated for efficacy and received whole brain fMRI scan before and after the treatment. The observation indicators included (1) NHISS score and its sub-item scores related to dyskinesia before and after treatment. (2) the amplitude of low-frequency fluctuations (ALFF), regional homogeneity (ReHo), and voxel-mirror homotopy connection (VMHC) analyzed based on Matlab 2012a platform, SPM12, and REST1.8 before and after treatment.Results: 1. The difference of NHISS scores between the two groups was statistically significant before and after treatment (P<0.05), indicates that patients in the treatment group were improved better. 2. The changes in brain area activation between the two groups of patients were as follows: (1) The values of VMHC increased in brain regions dominated by bilateral BA6 and BA8; (2) The values of ALFF increased mainly in the left BA39 and the adjacent superior temporal gyrus and middle temporal gyrus . (3) The values of ReHo increased in the brain region of left middle temporal gyrus (including BA21) and its extending to BA37, and the left BA40 and angular gyrus and its extending to BA7. Conclusion: Scalp acupuncture can help the recovery of the patients with acute infarction of superior middle cerebral artery and its mechanism possibly related to the special changes in the regional functional activities of sensory integration, language processing and motor coordination, also the bilateral motor control of frontal lobe.Trial registration: Trial Registration Number: Chinese Clinical Trial Registry ChiCTR-IOR-15007672. Registration date: November 7, 2015.
Thirty-Three Long-Term Survivors After Cytoreductive Surgery in Patients With Peritoneal Metastases From Colorectal Cancer: A Retrospective Descriptive Study
Authors: Kamada Yasuyuki , Hida Koya , Ishibashi Haruaki , Sako Shouzou , Mizumoto Akiyoshi , Ichinose Masumi , Padmanabhan Naveen , Yoshida Shinya , Yonemura Yutaka 
Published: November 2020

Abstract
BackgroundCytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) improves survival in selected patients with peritoneal metastasis (PM) from colorectal cancer (CRC). However, little has been reported on characteristics and clinical course of long-term survivors with CRC-PM beyond 5 years. The objective of this study was to describe the clinical and oncological features affecting long-term survival of CRC-PM after comprehensive treatment.MethodsBetween January 1990 and April 2015, CRC-PM patients who underwent CRS with or without HIPEC in two Japanese tertiary hospitals and who survived longer than 5 years after the first CRS for PM were retrospectively investigated. Clinicopathological parameters and therapeutic details involved in long-term survival were reviewed. Patients were defined as cured if the recurrence-free interval was > 5 years after the last operation for metastases.ResultsThirty-three patients with a median peritoneal cancer index (PCI) of 4 (range, 1–27) were included. Complete cytoreduction was achieved in all 33 patients, and none had a rectal primary. Recurrence was observed in 19 patients (57.6%) at a median of 2.6 (range, 0.7–7.4) years. Sixteen patients (48.5%) were considered cured, of whom two never developed re-recurrence after the second surgery. The median PCI of cured group was 2 (range, 1–8).ConclusionsLong-term survival and cure were obtained after CRS in selected patients with CRC-PM. Low PCI, complete cytoreduction, and non-rectal primary are associated with long-term survival and cure in PM from CRC.
Should We Think More About Chronic Osteomyelitis After Trauma? A Case Report of Osteomyelitis of the Cuboid Bone
Authors: Zhang Guang-hui , Wang Hong-jun , Song Bing , Wang Hong-bin 
Published: November 2020

Abstract
Osteomyelitis is a refractory infectious disease in surgery, which takes a long time to be treated and the condition is easy to be repeated, which causes a heavy burden to individuals and families. Actively looking for the cause is the key to the treatment of osteomyelitis. In our work, we encountered a patient with cuboid osteomyelitis who had forgotten the history of injury and had received surgical treatment before seeing a doctor in our hospital, which caused great difficulties to our work. In the work of diagnosis and treatment, we constantly adjust the treatment plan and treatment ideas, and finally remove the branches from the infected focus, and obtain satisfactory treatment results.
Three-Month Outcomes of Aortic Valve Reconstruction Using Collagenous Membranes (Biosheets) Produced by in Body Tissue Architecture in A Goat Model: A Preliminary Study
Authors: Okamoto Keitaro , Umeno Tadashi , Wada Tomoyuki , Shuto Takashi , Anai Hirofumi , Nishida Haruto , Nakayama Yasuhide , Miyamoto Shinji 
Published: November 2020

Abstract
Background: Autologous pericardium is widely used as a plastic material in intracardiac structures, in the pulmonary artery, and in aortic valve leaflets. For aortic valve reconstruction (AVRec) using the Ozaki procedure, it has produced excellent clinical results within a 10-year period. In-body tissue architecture (iBTA), which is based on the phenomenon of tissue encapsulation of foreign materials, can be used to prepare autologous prosthetic tissues. In this preliminary study, we examined whether biosheets can be used as valve leaflet material for glutaraldehyde-free AVRec by subchronic implantation experiments in goats and evaluated its performance compared with glutaraldehyde-treated autologous pericardium for AVRec. Methods: Biosheets were prepared by embedding molds for two months into the dorsal subcutaneous spaces of goats. Allogenic biosheets (n=4) cut into the shape of the valve were then implanted to the aortic valve annulus of four goats for three months without glutaraldehyde treatment. Autologous pericardium (n=4) was used as a control. Valve function was observed using echocardiography. Results: All goats survived the three-month study period. In biosheets, the leaflet surfaces were very smooth and, on histology, partially covered with a thin neointima (including endothelial cells). Biosheets were more thoroughly assimilated into the aortic root compared with autologous pericardium.Conclusions: For the first time, biosheets could be used for large animal AVRec without glutaraldehyde fixation. In this limited preliminary study, it was found that biosheets had superior engraftment and regeneration performance as an aortic valve leaflet material compared with autologous pericardium.
Morphology and Size Distribution of Naturally Occurring Asbestos using TEM in Landscape Rock in Parks
Authors: Chung Sook-Nye , Kim Jisung , Im Gikyo , Won Sunjung , Lee Jiyoung , Kim Gwangrae , Shin Jinho , Shin Yongseung 
Published: November 2020

Abstract
BackgroundAsbestos is a silicate mineral that is naturally generated by geological processes in the Earth's crust. The six types of commercially used asbestos each display the asbestiform crystal habit that is characterized by fibrous aggregates of extremely long, thin crystals. This study provided data that can be used to discriminate asbestiform and non-asbestiform amphiboles contained in landscaping rocks in parks. MethodIn five of the 21 parks, two or more landscaping rocks were found to contain asbestos. These five were selected for additional study. The length and width of fibers being at least 5 ㎛ long and with a minimum aspect ratio of 3:1 were measured using a Transmission Electron Microscope(TEM) and compared to a standard asbestos sample.ResultsThe park samples were thicker than 1 ㎛(average 1.9 ㎛) while the Health and Safety Executive(HSE) reference sample averaged 0.3 ㎛ in width with 100 % thinner than 1.0 ㎛. The average aspect ratios were 7.1 for the park samples and 67.1 for the HSE reference sample. The actinolite contained in the landscape rocks of the five selected parks did not show the typical asbestiform structure that was observed in the standard asbestos sample.ConclusionsBased on these distributions, the amphibole fibers in sampled landscape rocks were found to be thicker and lower aspect ratio than those of the standard sample. The result of this study can contribute to the public policy for managing and controlling of landscaping rocks containing naturally occurring asbestos(NOA) and also to communicate about the possible health risk resulting from NOA contained in landscaping rocks.
Is anti-HMGB1 Antibody a Potential Characteristic Autoantibody for Sjögren's Syndrome?
Authors: Chen Mingkun , Zhou Yi , Xue Miao , Zhu Rongrong , Jing Liling , Lin Liling , Qin Yanghua 
Published: November 2020

Abstract
BACKGROUND: Sjögren's syndrome (SS) is a common chronic inflammatory autoimmune disease, affects about 0.33% to 0.77% population in China. The positive for antinuclear antibodies (ANA) is one of the key features of SS, which show typical granular pattern in indirect immunofluorescent antibody test (IIFT). About 70% SS patients have the anti-SS-A and/or SS-B antibodies, which indicates other autoantibodies in SS patients.METHODS: The anti-HMGB1 antibody in 93 SS patients and 96 healthy controls were investigated with in-house built ELISA and immunoblotting; and the location of HMGB1 and fluorescent pattern of anti-HMGB1 antibody were investigated with IIFT. The contribution of anti-HMGB1 antibody in ANA-IF with Cas9-induce HMGB1 knockout cells were also detected.RESULTS: The anti-HMGB1 antibody in SS patients (9.96±5.55 VS 4.9±1.4 RU/ml RU/ml compared to healthy controls) increased. With ROC curve analysis, when taking 8 RU/ml as the cutoff value, the sensitivity, specificity, and the area under the curve were 64.5%, 96.9% and 0.83, respectively. A total of 18 patients (20.7%) with granular pattern in ANA-IF test were anti-HMGB1 antibody positive only. With commercial antibody, the fluorescent pattern of anti-HMGB1 antibody showed typical granular pattern. The serum from ANA-IF (+), SS-A (-), and SS-B (-) SS patients showed typical granular pattern in wildtype B16 cells, but no fluorescence in HMGB1 knockout B16 cells.CONCLUSIONS: Anti-HMGB1 antibody may be one of the characteristic autoantibodies of SS except anti-SS-A and SS-B. The detection of anti-HMGB1 antibody can provide more laboratory evidence for clinical diagnosis of SS.
Clinical Relevance for Circulating Cold Inducible RNA-Binding Protein (CIRP) in Patients with ASD
Authors: Fujita Yuya , Yago Toru , Asano Tomoyuki , Matsumoto Haruki , Matsuoka Naoki , Temmoku Jumpei , Sato Shuzo , Furuya Makiko , Suzuki Eiji , Watanabe Hiroshi , Kawakami Atsushi , Migita Kiyoshi 
Published: November 2020

Abstract
Background: Adult Still’s disease (ASD) is a systemic autoinflammatory disease, in which danger-associated molecular patterns (DAMPs)-mediated inflammasome activation seems to be involved in the disease pathogenesis. Cold inducible RNA-binding protein (CIRP) belongs to a family of cold-shock proteins that respond to cellular stress and has been identified as a DAMP that triggers the inflammatory response. The aim of this study is to investigate the clinical significance of serum CIRP levels in ASD.Methods: Serum samples were obtained from 42 patients with active ASD or 50 patients with rheumatoid arthritis (RA) and 15 healthy control patients (HCs). Serum levels of CIRP and IL-18 were determined using enzyme-linked immunosorbent assay and compared with 42 patients with ASD or 50 patients with RA and 15 HCs. Results were also analyzed according to the clinical features of ASD.Results: Serum CIRP levels were significantly higher in patients with ASD compared with patients with RA (median: 9.6 ng/mL, IQR [5.7–14.4] versus 3.2 ng/mL, IQR [1.9–3.8]; p < 0.001) and with HCs (2.8 ng/mL, [IQR; 1.4–4.9], p < 0.001). There was a significant positive correlation between serum CIRP levels and ASD disease activity score (Pouchet’s score r=0.45, p=0.003) as well as between ASD-specific biomarkers ferritin and IL-18. However, there was no significant difference in the serum CIRP levels among ASD patients with three different disease phenotypes. Intracellular CIRP expression on CD14+ monocytes was elevated in patients with ASD compared with those in patients in RA.Conclusion: These results suggest that CIRP may play a significant role in the pathophysiology of ASD and could be a potential biomarker for monitoring the disease activity of ASD.
TIMP-2 Regulates 5-Fu Resistance via the ERK/MAPK Signaling Pathway in Colorectal Cancer
Authors: Zhang Guolin , Luo Xin , Xu Jianbin , Zhang Wei , Chen Engeng , Meng Qing , Wang Di , Huang Xuefeng , Zhou Wei , Song Zhangfa 
Published: November 2020

Abstract
Background: 5-Fluorouracil (5-Fu) is the first-line chemotherapeutic drug in the treatment of colorectal cancer. The efficiency of 5-Fu is limited by drug resistance in colorectal cancer patients. This study was aimed to define the functions of tissue inhibitor metalloproteinases 2 (TIMP-2) in the 5-Fu resistance to colorectal cancer and investigate its potential mechanism.Methods: Cytokine array, ELISA and RT-qPCR were performed to detect cytokine expression levels. Western blot and immunohistochemistry were used to show the differential expression of proteins. In addition, cell viability was detected by CCK-8.Results: We established that there is an up-regulation in the expression of the TIMP-2 in colorectal cancer patients. This up-regulation in TIMP-2 expression was evident in 5-Fu resistant colorectal cancer patients and resulted in a poor prognosis. Besides, in vivo, clinical studies and patient-derived xenograft (PDX) models confirmed that TIMP-2 was highly expressed in the 5-Fu-resistant colorectal cancer. We deduced an autocrine mechanism through which elevated TIMP-2 protein levels sustained colorectal cancer cell resistance to 5-Fu by constitutively activating the ERK/MAPK signaling pathway via an autocrine mechanism. The 5-Fu resistance could overcome by the inhibition of TIMP-2 by anti-TIMP-2 antibody or ERK/MAPK by U0126.Conclusion: Our findings identify a TIMP-2-ERK/MAPK mediated 5-Fu resistance mechanism in colorectal cancer. Moreover, we recommend the use of an ERK/MAPK signal pathway inhibitor or TIMP-2-mediated immunotherapy for 5-Fu resistant colorectal cancer.
A Novel Recombinant Protein Vaccine Containing the Different E7 Proteins of the HPV16, 18, 6, 11 E7&nbsp;Linked to the HIV-1 Tat (47-57) Improve Cytotoxic Immune Responses
Authors: Mousavi Tahoora , Valadan Reza , Rafiei Alireza , Abbasi Ali , Haghshenas Mohammad Reza 
Published: November 2020

Abstract
Objective: Human papillomavirus infection (HPV) is the most common viral infection which is causes of cervical, penal, vulvar, anal and, oropharyngeal cancer. E7 protein of HPV is a suitable target for induction of T cell responses and controlling HPV-related cancer. The aim of the current study was to designed and evaluated a novel fusion protein containing the different E7 proteins of the HPV 16, 18, 6 and 11, linked to the cell-penetrating peptide HIV-1 Tat 49-57, in order to improve cytotoxic immune responses in in-vitro and in-vivo. Methods: In this study whole sequence of HPV16,18,6,11 E7-Tat (47-57) and HPV16,18,6,11 E7 cloned into the vector and expressed in E.coli (BL21). The purified protein was confirmed by SDS page and western blotting and then injected into the C57BL/6 mice. The efficiency of the fusion protein vaccine was assessed by antibody response assay, cytokine assay (IL-4 and IFN-γ), CD+8 cytotoxicity assay and tumor challenge experiment. Results: Result showed that fusion proteins containing Adjuvant (IFA,CFA) could express higher titer of antibody. Also, we showed that vaccination with E7-Tat and, E7-Tat-ADJ induced high frequencies of E7-specific CD8+ T cells and CD107a expression as well as IFN-γ level and enhanced long-term survival in the therapeutic animal models. Conclusion: Our finding suggested that this novel fusion protein vaccine was able to induce therapeutic efficacy and immunogenicity by improving CD8+ T cell in TC-1 tumor bearing mice; so this vaccine may be appreciated for research against HPV and tumor immunotherapies.
SUMO-Activating Enzyme Subunit 1 (SAE1) is A Promising Diagnostic Cancer Metabolism Biomarker of Hepatocellular Carcinoma
Authors: Ong Jiann Ruey , Bamodu Oluwaseun Adebayo , Khang Nguyen Viet , Lin Yen-Kuang , Yeh Chi-Tai , Lee Wei-Hwa , Cherng Yih-Giun 
Published: November 2020

Abstract
Background: Hepatocellular carcinoma (HCC) is one of the most diagnosed malignancies and a leading cause of cancer-related mortality globally. This is exacerbated by its highly aggressive phenotype, and limitation in early diagnosis and effective therapies. The SUMO-activating enzyme subunit 1 (SAE1) is a component of a heterodimeric small ubiquitin-related modifier that plays a vital role in SUMOylation, a post-translational modification involving in cellular events such as regulation of transcription, cell cycle and apoptosis. Reported overexpression of SAE1 in glioma in a stage-dependent manner suggests it probable role in cancer initiation and progression. Methods: In this study, hypothesizing that SAE1 is implicated in HCC metastatic phenotype and poor prognosis, we analyzed the expression of SAE1 in several cancer databases. Results: Here, we demonstrated that SAE1 is overexpressed in HCC samples compared to normal liver tissue, and this observed SAE1 overexpression is stage and grade-dependent and associated with poor survival. The receiver operating characteristic analysis of SAE1 in TCGA-LIHC patients (n=421) showed an AUC of 0.925, indicating an excellent diagnostic value of SAE1 in HCC. Our protein-protein interaction analysis for SAE1 showed that SAE1 interacted with and activated oncogenes such as PLK1, CCNB1, CDK4 and CDK1, while simultaneously inhibiting tumor suppressors including PDK4, KLF9, FOXO1 and NEDD4. Immunohistochemical staining and clinicopathological correlate analysis of SAE1 in our TMU-SHH HCC cohort (n =54) further validated the overexpression of SAE1 in cancerous liver tissues compared with ‘normal’ paracancerous tissue, and high SAE1 expression was strongly correlated with metastasis and disease progression. Conclusion: In conclusion, the present study demonstrates that SAE1 is a targetable molecular biomarker with high potential diagnostic and prognostic implications for patients with HCC.
Attitudes Towards and Compliance with the WHO Surgical Safety Checklist in China: A Multicentre Cross-Sectional Study
Authors: Tan Jie , Ngwayi James Reeves Mbori , Ding Zhaohan , Zhou Yufa , Li Ming , Chen Yujie , Hu Bingtao , Liu Jingping , Porter Daniel Edward 
Published: November 2020

Abstract
Background: Ten years after the introduction of Chinese Ministry of Health (MoH) version of Surgical Safety Checklist (SSC) we wished to assess the ongoing influence of the World Health Organisation (WHO) SSC by observing all three sections during elective surgical procedures in China, as well as to survey operating room staff more widely about the WHO SSC.Methods: A questionnaire was designed to gain authentic views on the WHO SSC. We also conducted a prospective cross-sectional study at five level 3 hospitals. Local data collectors were trained to document specific item performance. Adverse events which delayed the operation were recorded as well as the professionals leading or participating in the three SSC phases.Results: A total of 846 operating room professionals from 138 hospitals representing every mainland province responded to the survey. There was widespread acceptance of the checklist and its value in improving patient safety. 860 operations were observed for SSC compliance. Overall compliance was 79.8%. The ‘time-out’ phase compliance in surgeon-dependent items reduced when it was nurse-led (p<0.0001). WHO SSC interventions which are omitted from the MoH SSC continued to be discussed over half the time. Overall adverse events rate was 2.7%. One site had near 100% compliance in association with a circulating inspection team which had power of sanction.Conclusion: The WHO SSC remains a powerful tool for patient safety in China. Changes in behaviour for nurses (assertiveness) and surgeons (teamwork) could improve compliance. Random checks of compliance may have merit.
Plurilingualism and Monolingualism in Foreign Language Classrooms: The Perspective of EFL Teachers in Saudi Arabia
Authors: Alsaawi Ali 
Published: November 2020

Abstract
The idea of teaching a target language via a monolingual medium of instruction in the classroom has long predominated in the pedagogical context. In Saudi Arabia, excluding the students’ mother tongue (Arabic) in the foreign language classroom has been seen as a tool that accelerates the acquisition of the target language (English). This is widely viewed as the most practical and effective method of language learning, especially in the Gulf region, where English is a foreign language employed in the fields of economics and business. The recent academic argument that exploiting the students’ linguistic repertoire, including the mother tongue, in the target language classroom boosts and fosters the students’ learning cycle is still encountering huge resistance, especially among second/foreign language teachers. To explore this dispute from the perspective of English as a foreign language (EFL) teachers in intermediate and secondary schools, a case study was conducted with 34 teachers in the Qassim region, Saudi Arabia, through questionnaires and a focus group interview. The study found that most teachers believe that the policy of using the target language (English) only is the most effective method of language learning. They employed the students’ mother tongue (Arabic) on an ad hoc basis to ensure complete comprehension, organize classroom tasks or convey personal remarks. In addition, the study revealed that teachers’ understanding of plurilingualism was unclear and limited to the verbal use of two languages, and that EFL teachers need more clarification on its application in the classroom.