bluuebunny/crossref_metadata_2025_split huggingface dataset:
The alarmins S100A8 and S100A9 mediate acute pain in experimental synovitis
Authors: Blom Arjen B. , van den Bosch Martijn H. , Blaney Davidson Esmeralda N. , Roth Johannes , Vogl Thomas , van de Loo Fons A. , Koenders Marije , van der Kraan Peter M. , Geven Edwin J. , van Lent Peter L. 
Published: August 2020

Abstract
Background
Synovitis-associated pain is mediated by inflammatory factors that may include S100A8/9, which is able to stimulate nociceptive neurons via Toll-like receptor 4. In this study, we investigated the role of S100A9 in pain response during acute synovitis.

Methods
Acute synovitis was induced by streptococcal cell wall (SCW) injection in the knee joint of C57Bl/6 (WT) and S100A9−/− mice. The expression of S100A8/A9 was determined in serum and synovium by ELISA and immunohistochemistry. Inflammation was investigated by 99mTc accumulation, synovial cytokine release, and histology at days 1, 2, and 7. To assess pain, weight distribution, gait analysis, and mechanical allodynia were monitored. Activation markers in afferent neurons were determined by qPCR and immunohistochemistry in the dorsal root ganglia (DRG). Differences between groups were tested using a one-way or two-way analysis of variance (ANOVA). Differences in histology were tested with a non-parametric Mann–Whitney U test. p values lower than 0.05 were considered significant.

Results
Intra-articular SCW injection resulted in increased synovial expression and serum levels of S100A8/A9 at day 1. These increased levels, however, did not contribute to the development of inflammation, since this was equal in S100A9−/− mice. WT mice showed a significantly decreased percentage of weight bearing on the SCW hind paw on day 1, while S100A9−/− mice showed no reduction. Gait analysis showed increased “limping” behavior in WT, but not S100A9−/− mice. Mechanical allodynia was observed but not different between WT and S100A9−/− when measuring paw withdrawal threshold. The gene expression of neuron activation markers NAV1.7, ATF3, and GAP43 in DRG was significantly increased in arthritic WT mice at day 1 but not in S100A9−/− mice.

Conclusions
S100A8/9, released from the synovium upon inflammation, is an important mediator of pain response in the knee during the acute phase of inflammation.
Relationship between Upper Quarter Y Balance Test performance and throwing proficiency in adolescent Olympic handball players
Authors: Bauer Julian , Schedler Simon , Fischer Stephan , Muehlbauer Thomas 
Published: August 2020

Abstract
Background
Olympic handball is a sport mainly focused on executing throwing and passing techniques with the throwing arm. Functional specialization due to the unilateral characteristic and dominance of the throwing arm may lead to adapted control of shoulder stability and mobility. Thus, we examined side differences between the throwing and the non-throwing arm. Additionally, correlations between the Upper Quarter Y Balance Test (YBT-UQ) and handball-specific performance measures were investigated.

Methods
All participants (F = 13 yrs., n = 14, training experience [te] 5.9 ± 1.3 yrs.; M = 14 yrs., n = 24, te 6.5 ± 2.5 yrs.; M = 15 yrs., n = 18, te 9.3 ± 2.2 yrs) were Olympic handball players of a regional youth selection team. YBT-UQ was executed assessing performance in medial, inferolateral and superolateral reach direction normalized to the upper limb length together with a composite score of the mean of all reach directions. A radar gun and a target net were used for the assessment of throwing velocity and throwing accuracy. The paired t-test was used to detect side differences in YBT-UQ performance. Pearson’s correlation analysis was calculated for associations between YBT-UQ and throwing velocity/accuracy.

Results
Significant differences between the throwing and non-throwing arm were only detected for the superolateral reach direction in the 14-year-old males. Small correlations between YBT-UQ performance and throwing velocity/accuracy (13-year-old females: − 0.01 ≤ r ≤ − 0.37 / 0.01 ≤ r ≤ 0.31; 14-year-old males: 0.10 ≤ r ≤ 0.45 / -0.01 ≤ r ≤ .-0.51; 15-year-old males: 0.06 ≤ r ≤ 0.34 / 0.01 ≤ r ≤ − 0.45) were observed, irrespective of age and sex category.

Conclusions
There was only a minimal difference in performance of the YBT-UQ between the throwing and non-throwing arm and only weak if any relationships between throwing performance and stability/mobility of the upper extremities in adolescent Olympic handball players existed. Further research is needed to investigate whether the YBT-UQ is a useful tool to detect training-related improvements in measures of shoulder stability/mobility and functional performance.
Vibroarthrographic analysis of patellofemoral joint arthrokinematics during squats with increasing external loads
Authors: Ołowiana Ewelina , Selkow Noelle , Laudner Kevin , Puciato Daniel , Bączkowicz Dawid 
Published: August 2020

Abstract
Background
The patellofemoral joint (PFJ) provides extremely low kinetic friction, which results in optimal arthrokinematic motion quality. Previous research showed that these friction-reducing properties may be diminished due to the increase in articular contact forces. However, this phenomenon has not been analyzed in vivo during functional daily-living activities. The aim of this study was the vibroarthrographic assessment of changes in PFJ arthrokinematics during squats with variated loads.

Methods
114 knees from 57 asymptomatic subjects (23 females and 34 males) whose ages ranged from 19 to 26 years were enrolled in this study. Participants were asked to perform 3 trials: 4 repetitions of bodyweight squats (L0), 4 repetitions of 10 kg barbell back loaded squats (L10), 4 repetitions of 20 kg barbell back loaded squats (L20). During the unloaded and loaded (L10, L20) squats, vibroarthrographic signals were collected using an accelerometer placed on the patella and were described by the following parameters: variation of mean square (VMS), mean range (R4), and power spectral density for frequency of 50–250 Hz (P1) and 250–450 Hz (P2).

Results
Obtained results showed that the lowest values were noted in the unloaded condition and that the increased applied loads had a significant concomitant increase in all the aforementioned parameters bilaterally (p < 0.05).

Conclusion
This phenomenon indicates that the application of increasing knee loads during squats corresponds to higher intensity of vibroacoustic emission, which might be related to higher contact stress and kinetic friction as well as diminished arthrokinematic motion quality.
Mechanical stimulation of human dermal fibroblasts regulates pro-inflammatory cytokines: potential insight into soft tissue manual therapies
Authors: Anloague Aric , Mahoney Aaron , Ogunbekun Oladipupo , Hiland Taylor A. , Thompson William R. , Larsen Bryan , Loghmani M. Terry , Hum Julia M. , Lowery Jonathan W. 
Published: August 2020

Abstract
Objective
Soft tissue manual therapies are commonly utilized by osteopathic physicians, chiropractors, physical therapists and massage therapists. These techniques are predicated on subjecting tissues to biophysical mechanical stimulation but the cellular and molecular mechanism(s) mediating these effects are poorly understood. Previous studies established an in vitro model system for examining mechanical stimulation of dermal fibroblasts and established that cyclical strain, intended to mimic overuse injury, induces secretion of numerous pro-inflammatory cytokines. Moreover, mechanical strain intended to mimic soft tissue manual therapy reduces strain-induced secretion of pro-inflammatory cytokines. Here, we sought to partially confirm and extend these reports and provide independent corroboration of prior results.

Results
Using cultures of primary human dermal fibroblasts, we confirm cyclical mechanical strain increases levels of IL-6 and adding long-duration stretch, intended to mimic therapeutic soft tissue stimulation, after cyclical strain results in lower IL-6 levels. We also extend the prior work, reporting that long-duration stretch results in lower levels of IL-8. Although there are important limitations to this experimental model, these findings provide supportive evidence that therapeutic soft tissue stimulation may reduce levels of pro-inflammatory cytokines. Future work is required to address these open questions and advance the mechanistic understanding of therapeutic soft tissue stimulation.
Epidural analgesia in ICU chest trauma patients with fractured ribs: retrospective study of pain control and intubation requirements
Authors: Bachoumas Konstantinos , Levrat Albrice , Le Thuaut Aurélie , Rouleau Stéphane , Groyer Samuel , Dupont Hervé , Rooze Paul , Eisenmann Nathanael , Trampont Timothée , Bohé Julien , Rieu Benjamin , Chakarian Jean-Charles , Godard Aurélie , Frederici Laura , Gélinotte Stephanie , Joret Aurélie , Roques Pascale , Painvin Benoit , Leroy Christophe , Benedit Marcel , Dopeux Loic , Soum Edouard , Botoc Vlad , Fartoukh Muriel , Hausermann Marie-Hélène , Kamel Toufik , Morin Jean , De Varax Roland , Plantefève Gaetan , Herbland Alexandre , Jabaudon Matthieu , Duburcq Thibault , Simon Christelle , Chabanne Russell , Schneider Francis , Ganster Frederique , Bruel Cedric , Laggoune Ahmed-Saïd , Bregeaud Delphine , Souweine Bertrand , Reignier Jean , Lascarrou Jean-Baptiste 
Published: August 2020

Abstract
Background
Nonintubated chest trauma patients with fractured ribs admitted to the intensive care unit (ICU) are at risk for complications and may require invasive ventilation at some point. Effective pain control is essential. We assessed whether epidural analgesia (EA) in patients with fractured ribs who were not intubated at ICU admission decreased the need for invasive mechanical ventilation (IMV). We also looked for risk factors for IMV.

Study design and methods
This retrospective, observational, multicenter study conducted in 40 ICUs in France included consecutive patients with three or more fractured ribs who were not intubated at admission between July 2013 and July 2015.

Results
Of the 974 study patients, 788 were included in the analysis of intubation predictors. EA was used in 130 (16.5%) patients, and 65 (8.2%) patients required IMV. Factors independently associated with IMV were chronic respiratory disease (P = 0.008), worse SAPS II (P < 0.0001), flail chest (P = 0.02), worse Injury Severity Score (P = 0.0003), higher respiratory rate at admission (P = 0.02), alcohol withdrawal syndrome (P < 0.001), and noninvasive ventilation (P = 0.04). EA was not associated with decreases in IMV requirements, median numerical rating scale pain score, or intravenous morphine requirements from day 1 to day 7.

Conclusions
EA was not associated with a lower risk of IMV in chest trauma patients with at least 3 fractured ribs, moderate pain, and no intubation on admission. Further studies are needed to clarify the optimal pain control strategy in chest trauma patients admitted to the ICU, notably those with severe pain or high opioid requirements.
Autogenous Shrinkage and Crack Resistance of Carbon Nanotubes Reinforced Cement-Based Materials
Authors: Liu Yanming , Shi Tao , Zhao Yujing , Gu Yuan , Zhao Zhifang , Chen Jiabin , Zheng Bingmiao , Shi Shichong 
Published: August 2020

AbstractCracking caused by shrinkage deformation of cement-based materials at early age is a major problem leading to material failure in restrained conditions. Carbon nanotubes (CNTs) are incorporated into cement-based materials, and the autogenous shrinkage and crack resistance of the new composite materials obtained by linear shrinkage and ring tests are studied to solve the destruction of the materials caused by the shrinkage of cement-based materials. The results showed that addition of CNTs significantly inhibited the autogenous shrinkage of cement-based materials with maximum reduction rate above 40%. CNTs also significantly improved the cracking resistance of cement-based materials. The optimal effect was noticed at CNTs content of 0.1 wt%. The incorporation of CNTs not only inhibits the autogenous shrinkage of cement-based materials, but also inhibits the drying shrinkage of cement-based materials to some extent. Therefore, carbon nanotubes have the potential to solve the destruction of materials caused by shrinkage of cement-based materials.
Unclassified four-repeat tauopathy associated with familial parkinsonism and progressive respiratory failure
Authors: Nakano Masayoshi , Riku Yuichi , Nishioka Kenya , Hasegawa Masato , Washimi Yukihiko , Arahata Yutaka , Takeda Akinori , Horibe Kentaro , Yamaoka Akiko , Suzuki Keisuke , Tsujimoto Masashi , Li Yuanzhe , Yoshino Hiroyo , Hattori Nobutaka , Akagi Akio , Miyahara Hiroaki , Iwasaki Yasushi , Yoshida Mari 
Published: August 2020

AbstractWe describe an autopsied patient with familial parkinsonism and unclassified four repeat-tau (4R-tau) aggregation. She presented with bradykinesia, truncal dystonia, and mild amnesia at the age of 61 and then exhibited body weight loss (15 kg over 8 months), sleep disturbances, and progressive respiratory failure with CO2 narcosis. She died of respiratory failure at the age of 62, 14 months after disease onset. Her brother also showed parkinsonism at the age of 58 and suddenly died 6 months later. Postmortem examination revealed 4R-tau aggregation, which was characterized by neuronal globose-type tangles or pretangles, bush-like or miscellaneous astrocytic inclusions, and coiled bodies. The temporal tip, the striatum, the substantia nigra, the tegmentum of the midbrain, the medullary reticular formation, and the spinal cord were severely involved with tau aggregation. Argyrophilic grains and ballooned neurons were also found in the medial temporal structures, however, extensions of the 4R-aggregations in the case were clearly broader than those of the argyrophilic grains. Western blot analysis of sarkosyl-insoluble fractions from brain lysates revealed prominent bands of tau at both 33 kDa and 37 kDa. Genetic examinations did not reveal any known pathogenic mutations in MAPT, DCTN-1, PSEN-1, or familial or young-onset parkinsonism-related genes. The clinical manifestations, pathologic findings, and biochemical properties of aggregated tau in our patient cannot be explained by argyrophilic grain disease or other known 4R-tauopathies alone. Our results further extend the clinical and neuropathologic spectra of 4R-tauopathy.
Soybean fermentation with basidiomycetes (medicinal mushroom mycelia)
Authors: Suruga Kohei , Tomita Tsuyoshi , Kadokura Kazunari 
Published: August 2020

AbstractBackgroundEdible mushroom fruiting bodies and their mycelia have become attractive functional foods. Mushroom mycelia have been investigated for their potential food applications. Here, soybeans were fermented using medical mushroom mycelia fromGanoderma lucidum,Hericium erinaceus, andHericium ramosumto develop novel functional food materials for human health.ResultsWild mushroom fruiting bodies were collected from nature to isolate their mycelia. Soybeans were fermented using mushroom mycelia for 4 weeks. The antioxidant activity of fermented soybeans was analysed, and fermented soybean compounds were determined using HPLC and LC/MS analysis. Antioxidant and alpha-glucosidase inhibitory activities of fermented soybean mycelia were more potent than the control group. The volume and type of isoflavones significantly differed between soybean fermentation byGanoderma lucidum,Hericium erinaceus,andHericium ramosummycelia, based on HPLC and LC/MS analysis.ConclusionWe used mushroom mycelia to uncover new information regarding fermented soybean. Soybean fermentation using mushroom mycelia could be useful as a novel bioactive food material or nutritional supplement.
The urgent need for disability studies among midlife adults
Authors: Karvonen-Gutierrez Carrie A. , Strotmeyer Elsa S. 
Published: August 2020

AbstractIssues of poor physical functioning and disability are burdensome for midlife adults and evidence suggests that the prevalence of these conditions is increasing temporally. Physical functioning during the midlife period, however, may be highly amendable to intervention given the highly dynamic nature of functioning during this life stage. Thus, efforts to improve or forestall poor physical functioning and/or disability during midlife may not only improve the health status and quality of life for midlife adults but may have important ramifications on the health of these individuals who will become older adults in the future. This thematic series on women and disability includes contributions addressing issues of person, place and time with respect to disability in midlife and into late adulthood. The purpose of this commentary is to provide a summary overview of the major themes of the series and to offer insight into areas of most promise for intervention among midlife populations to improve physical functioning and prevent disability.
Establishment and effectiveness evaluation of a scoring system for exertional heat stroke by retrospective analysis
Authors: Yang Meng-Meng , Wang Lu , Zhang Yu , Yuan Rui , Zhao Yan , Hu Jie , Zhou Fei-Hu , Kang Hong-Jun 
Published: August 2020

AbstractBackgroundHeat stroke (HS) is a serious, life-threatening disease. However, there is no scoring system for HS so far. This research is to establish a scoring system that can quantitatively assess the severity of exertional heat stroke (EHS).MethodsData were collected from a total of 170 exertional heat stroke (EHS) patients between 2005 and 2016 from 52 hospitals in China. Univariate statistical methods and comparison of the area under the receiver operating characteristic (ROC) curve (AUC) were used to screen exertional heat stroke score (EHSS) parameters, including but not limited body temperature (T), Glasgow Coma Scale (GCS) and others. By comparing the sizes of the AUCs of the APACHE II, SOFA and EHSS assessments, the effectiveness of EHSS in evaluating the prognosis of EHS patients was verified.ResultsThrough screening with a series of methods, as described above, the present study determined 12 parameters – body temperature (T), GCS, pH, lactate (Lac), platelet count (PLT), prothrombin time (PT), fibrinogen (Fib), troponin I (TnI), aspartate aminotransferase (AST), total bilirubin (TBIL), creatinine (Cr) and acute gastrointestinal injury (AGI) classification – as EHSS parameters. It is a 0–47 point system designed to reflect increasing severity of heat stroke. Low (EHSS< 20) and high scores (EHSS> 35) showed 100% survival and 100% mortality, respectively. We found that AUCEHSS > AUCSOFA > AUCAPACHE II.ConclusionA total of 12 parameters – T, GCS, pH, Lac, PLT, PT, Fib, TnI, AST, TBIL, Cr and gastrointestinal AGI classification – are the EHSS parameters with the best effectiveness in evaluating the prognosis of EHS patients. As EHSS score increases, the mortality rate of EHS patients gradually increases.
Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba)
Authors: Yesmin Samina , Paul Arkajyoti , Naz Tarannum , Rahman A. B. M. Atiqur , Akhter Sarkar Farhana , Wahed Mir Imam Ibne , Emran Talha Bin , Siddiqui Shafayet Ahmed 
Published: August 2020

AbstractBackgroundThis experiment is conducted to evaluate the anti-inflammatory effect ofPiper chabaroots.MethodsThe in-vitro anti-inflammatory activity ofPiper chabawas carried out by human red blood cell (HRBC) membrane stabilization method which includes heat-induced hemolysis and hypo tonicity- induced hemolysis and also by another method of egg albumin denaturation assay.ResultsAnti-inflammatory activity study of crude ethanolic extract was performed using heat induced membrane stabilization method, hypo-tonicity induced HRBC membrane stabilization method and egg albumin denaturation method. Crude ethanolic extracts ofP. chabashowed promising in vitro anti-inflammatory activity in a concentration dependent manner. Using acetyl salicylic acid (ASA) as standard drug and was compared with ethanolic extract to determine anti-inflammatory activity. Heat induced anti-inflammatory test revealed that crude ethanolic extract ofP. chaba(500 μg/ml) and positive control ASA(500 μg/ml) have 52.667% and 78% respectively, hypo tonicity induced anti-inflammatory test showed 35.67% and 59% inhibition of red blood cell (RBC) hemolysis. Egg albumin denaturation method also evaluated that crude ethanolic extract (1000 μg/ml) and ASA (1000 μg/ml) showed 60% and 97.12% inhibition of egg albumin denaturation.ConclusionThe plant ofP. chabaof the genusPiperpossesses promising anti-inflammatory activities.
Resistance to antihypertensive drugs targeting Renin-Angiotensin-Aldosterone-System in cancer patients: a case series
Authors: Goel Mishita , Varandani Rajiv Sunil , Okwuosa Tochukwu M. 
Published: August 2020

AbstractHypertension impacts overall prognosis in cancer patients. There are no specific recommendations for its management in these patients. We report a case series of 5 cancer patients with suboptimal BP lowering and even worsening BP with ACEi or ARBs that improved to normal upon discontinuation of these drugs.
Recent studies on probiotics as beneficial mediator in aquaculture: a review
Authors: Hasan Kazi Nurul , Banerjee Goutam 
Published: August 2020

AbstractBackgroundThe diseases in fish and other economic aquatic species is a great concern, and every year it causes a huge loss in aquaculture sectors. The use of probiotics might be a good option to reduce the disease risk and to enhance the productivity.MethodsWe have gathered information from various important research and review articles related to fish diseases, probiotics, and gut microbial community. We have tried our level best to represent the up-to-date information in a concise manner.ResultsIn this present review, we have demonstrated the various beneficial aspects of probiotics in aquaculture sectors. Probiotics are considered as novel functional agents that have potential implications in influencing the gut microbiota of any aquatic organism. Researchers have already documented that probiotics play a wide spectrum functions (such as decrease diseases and stress, enhance immunity, modulate gut microbiota, helps in nutrition, improve water quality, etc.) in host body. Furthermore, the beneficial effects of probiotics contribute to increase feed value and growth of the animal, and improve spawning and hatching rate in aquaculture system. Here, we have discussed each and every functions of probiotics and tried to correlate with the previous knowledge.ConclusionThe reports regarding the efficacy of probiotics and its detailed mechanism of action are scarce. Till date, several probiotics have been reported; however, their commercial use has not been implicated. Most of the studies are based on laboratory environment and thus the potentiality may vary when these probiotics will be used in natural environments (pond and lakes).