bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Development and Psychometric Validation of A Low-Cost Anthropomorphic 3D-Printout Simulator for Training Basic Skills Applicable to Office-Based Hysteroscopy
Authors: Panazzolo Ana Rita Peixoto , Grando Leisa Beatriz , Volpato Lia Karina , Filho Getúlio R. de Oliveira 
Published: May 2020

Abstract
Background Hysteroscopy training requires the development of specific psychomotor skills. Few validated low-cost models exist in hysteroscopy. The main objective of this study is to determine face, content and construct validity of a simulator designed for training basic hysteroscopy skills applied to office-based hysteroscopy.Methods Twenty-five hysteroscopy experts and 30 gynecology residents participated in this prospective observational study. The simulator consisted of three color-textured, silicone-coated anthropomorphic 3-dimensional (3-D) printout uterine models inside a box. Each uterine model in the simulator was designed to developed one of the following basic hysteroscopic skills: hysteroscopic navigation, direct biopsy, and foreign body removal. Participants performed five video-recorded simulation attempts on each model. Procedure-specific checklists were used to rate performance.Results Median scores (25th – 75th percentiles; p-value) 4 (3–4; p < 0.001) and surgical experience associated to the simulated procedures 4 (3–4; p < 0.001) indicated positive perceptions as to the realism of the internal cavity of the simulator. Median scores of 4 (3–4; p < 0.001) were assigned to the realism and utility of the tasks performed in the simulator for enhancing novice training in hysteroscopy. Expert performance scores were significantly higher and task completion times were significantly lower than those of novices in the navigation exercise (F(1,53) = 56.66; p < 0.001), the directed biopsy exercise (F(1,53) = 22.45; p < 0.001), and the foreign body removal exercise (F(1,53) = 58.51; p < 0.001). Novices´ performance improved on all three exercises: navigation exercise (F(1,53) = 182.44; p < 0.001); directed endometrial biopsy (F(1,53) = 110.53; p < 0.001); and foreign body removal (F(1,53) = 58.62; p < 0.001). Experts´ task completion times were significantly lower when compared to that of novices in the five attempts (p < 0,001) of the exercises: navigation (F(1,48) = 25.46; p < 0.001); directed biopsy (F(1,46) = 31.20; p < 0.001); and foreign body removal (F(1,50) = 69.8; p < 0.001). Novices´ task completion times diminished significantly throughout the sequence of exercises.Conclusions The low-cost simulator designed for the acquisition of basic skills in hysteroscopy demonstrated face, content and construct validity.
DEAH-box polypeptide 32 promotes hepatocellular carcinoma progression via activating the β-catenin pathway
Authors: Hu Xiaoyun , Yuan Guosheng , Li Qi , Huang Jing , Cheng Xiao , Chen Jinzhang 
Published: May 2020

Abstract
Background
Hepatocellular carcinoma (HCC) is a refractory cancer with high morbidity and high mortality. It has been reported that DEAH-box polypeptide 32 (DHX32) was upregulated in several types of malignancies and predicted poor prognosis, which was associated with tumor growth and metastasis. However, the expression of DHX32 in HCC and its role in HCC progression remain largely unknown.
Methods
Western blot and RT-PCR assays were used to detect the expression of DHX32 and epithelial mesenchymal transition (EMT)-related genes in HCC cells. Wound-healing and Transwell invasion assays were performed to determine the effect of DHX32 and β-catenin on the migration and invasion of HCC cells. Cell proliferation was examined by EdU cell proliferation assay.
Results
In our study, we found that high level of DHX32 expression was associated with reduced overall survival in HCC patients. DHX32 expression was upregulated in human HCC cells and ectopic expression of DHX32 induced EMT, promoted the migration, invasion, and proliferation of HCC cells, and enhanced tumor growth. Silencing DHX32 reversed EMT, inhibited the migration, invasion, and proliferation of HCC cells, and suppressed tumor growth. RT-PCR assay revealed that DHX32 regulated the expression of CTNNB1, CCND1, COX2, MMP7, and WIF1 in HCC cells. Mechanistic investigations showed that silencing DHX32 decreased the expression of β-catenin in nucleus and β-catenin siRNA abrogated DHX32-mediated EMT, migration, invasion, and proliferation in HCC cells.
Conclusions
Our data suggested that DHX32 was an attractive regulator of HCC progression and indicated DHX32 can serve as a potential biomarker and therapeutic target for HCC patients.
Clinical characteristics and outcome of influenza virus infection among adults hospitalized with severe COVID-19: A retrospective cohort study from Wuhan, China
Authors: Tong Xunliang , Xu Xiaomao , Lv Guoyue , Wang He , Cheng Anqi , Wang Dingyi , Li Yanming 
Published: May 2020

Abstract
Background

Coronavirus disease 2019 (COVID-19) is an emerging infection disease that rapidly spreads worldwide. Clinical features and outcomes of severe COVID-19 patients with influenza virus IgM positive during the influenza season need to be described.
Methods

Retrospective cohort study of 140 patients with severe COVID-19 hospitalized in designated wards of Sino-French New City Branch of Tongji Hospital between Feb 8th and March 15th in Wuhan, Hubei province, China. The demographic, clinical feature, laboratory, treatment and outcome were collected and analyzed.
Results

Of 140 severe COVID-19 hospitalized patients, 73 patients were with median age of 66 years old with identification of influenza virus IgM-positive and 67 patients were with median age of 62 years old in influenza virus IgM-negative group. Nearly half of severe COVID-19 patients in this research are male. Majority of the severe COVID-19 patients had chronic underlying conditions. Wheeze was the clinical feature of severe COVID-19 patients with influenza IgM-positive (26.4% vs 9.0%, P = 0.008). On contrary, fatigue or myalgia was the feature of the COVID-19 patients without IgM-positive (38.4% vs 58.2%, P = 0.019). In laboratory examination, increased levels of ferritin and prolonging APTT were showed in severe COVID-19 patients without influenza IgM-positive compared with patients in the other group with significant differences. Death rate in the group of severe COVID-19 patients with influenza IgM-positive is higher than it is in other group with significant differences (14.9% vs 4.1%, P = 0.040). In univariate regression analysis, several factors were associated with higher risk of death, which included LDH, troponin, NT-proBNP, D-dimer, PT, APTT, lymphocytes, platelet and eGFR. However, influenza virus IgM positive was associated with lower risk of death. Multivariate Regression analysis showed that troponin and lymphocyte were independently associated with higher risk of death.
Conclusion

The characteristics of patients hospitalized with severe COVID-19 with identification of influenza virus IgM-positive were described. It hints proof of seasonal influenza which may overlap with COVID-19 and may cause a crisis we could confront in the future.
Clinical analysis of 207 patients with coronavirus disease 2019 (COVID-19) in Shanghai, China
Authors: Wang Lin , Zhang Yuan , Shan Fei , Zhang Renyin , Shi Nannan , Liu Fengjun , Shi J , Shi Yuxin 
Published: May 2020

Abstract
Objectives: To investigate clinical features and the chest computed tomography (CT) findings in patients with confirmed cases of coronavirus disease 2019 (COVID-19) in Shanghai. Materials and Methods: Two hundred seven patients (102 men and 105 women, 15-84 years old) with COVID-19 from 23 January 2020 to 8 February 2020 were retrospectively reviewed. The imaging findings, clinical and laboratory data of the patients were evaluated and analyzed. The CT score was determined by totaling the lobes of lungs affected ranging from 0-25. Results: The median time from onset of symptoms to first hospital admission was 5.3±3.9 days.After being tested positive, the hospital stay of patients with onset of symptoms within one week is longer than that of patients with onset of symptoms over one week (15.7 vs. 11.5 respectively, p<0.01). The initial lung findings of patients with COVID-19 on chest CT were small subpleural ground glass opacities (GGO) that grew larger with crazy-paving pattern and consolidation with or without interstitial opacity. The mean CT scores peaked at 8-10 days of illness, with a slow decline thereafter and substantial scores after the 10 days. Both age and CD4+ cell counts had a remarkable prognostic effect on imaging outcomes (p<0.05). Conclusion: For patients in mild-to-moderatecondition, the disease began to improve after 10 days from the initiation of the symptoms. Both age and baseline CD4+ cell count were pivotal predictor of the outcome of imaging of the patients with COVID-19.
FOXP3 inhibits tumor growth and induced apoptosis in hepatocellular carcinoma by targeting c-Myc
Authors: Gong Zhongqin , Jia Hao , Yu Jianqing , Liu Yi , Ren Jianwei , Yang Shengli , Hu Baoguang , Liu Liping , Lai Paul BS , Chen George Gong 
Published: May 2020

Abstract
Background

The status of FOXP3 and its isoforms in hepatocellular carcinoma (HCC) is unclear. We aimed to investigate the expression and function of FOXP3 and its isoforms in HCC.
Methods

The study was performed on 84 HCC patients, HCC cell lines and a mouse tumor model. The levels of FOXP3 and its isoforms were determined by nested PCR, quantitative real-time PCR and immunohistochemistry (IHC) staining. The correlation between their levels and clinicopathologic characteristics was analyzed.
Results

The full length of FOXP3 (FOXP3) and exon 3-deleted FOXP3 (FOXP3Δ3) were found to be the major isoforms in HCC. The levels of FOXP3Δ3 mRNA and protein in HCC tumor samples were not significantly different from their adjacent normal tissues. The high expression of FOXP3 protein in HCC patients showed a good overall survival. The overexpression of FOXP3 significantly reduced tumor cell proliferation, migration and invasion. The immunofluorescence result indicated that FOXP3 needed to be translocated into the nucleus to exert its inhibitory function. The luciferase assay demonstrated that FOXP3 could be synergistic with Smad2/3/4 to inhibit the oncogene c-Myc. The co-immunoprecipitation results further revealed that FOXP3 could interact with Smad2/3/4. The chromatin immunoprecipitation (ChIP) assay showed that both FOXP3 and Smad2/3/4 bound the promoter of the c-Myc to inhibit it. The in vivo mouse tumor model study confirmed the inhibitory effect of FOXP3.
Conclusion

Collectively, the expression of tumor FOXP3 can inhibit the growth of HCC via suppressing c-Myc directly or indirectly via interacting with Smad2/3/4. Therefore, FOXP3 is a tumor suppressor in HCC.
A Network-Based Approach to Deciphering A Dynamic Microbiome’s Response to A Subtle Perturbation
Authors: Shaw Grace Tzun-Wen , Liu An-Chi , Weng Chieh-Yin , Chen Yi-Chun , Chen Cheng-Yu , Weng Francis Cheng-Hsuan , Wang Daryi , Chou Chu-Yang 
Published: May 2020

Abstract
Background: Over the past decades, one main issue that has emerged in ecological and environmental research is how losses in biodiversity influence ecosystem dynamics and functioning, and consequently human society. Declines in biodiversity lead to more than just species loss. The exponentially growing human population and its exploitation of natural resources affect the environment from local to global scales via climate change, global warming, and other human-driven environmental problems. Although biodiversity is a common indicator of ecosystem functioning, it is difficult to measure biodiversity in microbial communities exposed to moderate or chronic environmental perturbations. Consequently, there is a need for alternative bioindicators to detect, measure, and monitor gradual changes in microbial communities against these slight, chronic, and continuous perturbations.Results: Recent studies have proven that co-occurrence patterns are effective for measuring responses to subtle perturbations. In this study, we mimicked dynamic microbial ecosystems under pressure from subtle perturbations by adding a key microbial species and comparing network topologies. The present study is a comprehensive investigation of how a network-oriented method can be used to track microbiome dynamics after a key species, S. acidaminiphila, is added into anaerobic digestion reactors of swine manure. Microbial networks before and after bioaugmentation with S. acidaminiphila showed diverse topological niches and 4-node motifs in which microbes with co-occurrence patterns played the central roles in regulating and adjusting the intertwined relationships among microorganisms in response to the subtle environmental changes.Conclusions: This study demonstrates that microbial networks are a good bioindicator for chronic perturbation and should be applied in a variety of ecological investigations.
Effectiveness of neuromuscular electrical stimulation for interstitial cystitis: a protocol of systematic review and meta-analysis
Authors: Chen Da-yin , Guo Ying-xue , Dong Long-xin , He Wen-jie , Cao Hui-feng , Wang Ping , Yue Cai-fang 
Published: May 2020

Abstract
Background This study will examine the effectiveness and safety of neuromuscular electrical stimulation (NMES) for the treatment of patients with interstitial cystitis (IC). Methods We will retrieve the following electronic databases from their commencements to the March 1, 2020 to discover all related potential studies: MEDLINE, EMBASE, Cochrane Library, Web of Science, CINAHL, China National Knowledge Infrastructure, Chinese Biomedical Literature Database, Chinese Scientific Journal Database, and WANFANG Database. Randomized controlled trials (RCTs) related to the NMES for the treatment of patients with IC will be included, regardless publication status and language. Literature selection, data collection, and study quality assessment will be independently performed by two authors. The extracted data will be expressed as risk ratio and 95% confidence intervals (CIs) for dichotomous data, and mean difference or standard mean difference and 95% CIs for continuous data. RevMan V.5.3 software will be employed for statistical analysis.Discussion This study will summarize current high quality RCTs to appraise the effectiveness and safety of NMES for the treatment of patients with IC. The findings of this study will provide helpful evidence to determine whether NMES is an effective treatment for patients with IC or not. Systematic review registration PROSPERO CRD42020170495.
Identification of key genes and their microRNAs in glioma: Evidence from bioinformatic analysis
Authors: Wen Xichao , Fu Meijuan , Wu Wensong , Zeng Zhaomu , Zheng Kebin 
Published: May 2020

Abstract
Background

Glioma is one of the most common primary intracranial tumors. Although a lot of studies have been conducted to elucidate the pathogeny of glioma, the molecular mechanisms are still unclear because of its complex biological functions.
Methods

To identify the candidate genes in the carcinogenesis and progression of glioma, microarray datasets GSE4290, GSE122498 and GSE2223 were downloaded from Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified, and performed function enrichment of DEGs by Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The protein-protein interaction network (PPI) was constructed using STRING and Cytoscape to find hub genes. Survival analysis and GEPIA database was conducted to screen and validate critical genes. Analysis of miRNA and genetic alteration was used to explore and predict the molecule mechanism.
Results

A total of 150 DEGs were identified, consisting of 54 downregulated genes and 96 upregulated genes. The enriched functions and pathways of the DEGs include regulation of transportation, synaptic transmission and SH3 domain binding. Fifteen hub genes were identified and biological process analysis revealed that these genes were mainly enriched in SH3 domain binding, neuron projection terminus, mitotic nuclear division, condensed chromosome and affected the brain development. Survival analysis showed that VAMP2, PPP3CA, DLGAP5, KIF14, REPS2, CENPU, KNTC1 and SMC4, may be involved in the carcinogenesis, invasion or recurrence of glioma. These 8 hub genes, which were related miRNAs and genetic changes were commonly involved in the development of glioma, were closely associated with tumor grade.
Conclusion

DEGs and hub genes identified in the present study help us understand the molecular mechanisms of carcinogenesis and progression of glioma, and provide candidate targets for diagnosis and treatment of glioma.
Effect of High Intensity Low Volume and High Intensity Moderate Volume Tapering Training on Mood Disturbance
Authors: Amede Ambachew , Yassin Rehima 
Published: May 2020

Abstract
Objective- The researchers aimed to investigate the effect of high intensity low volume and high intensity moderate volume tapering strategies on psychological traits in endurance athletes. Methodology- Thirty-seven young endurance athletes (mean age: 20±1.97 years; mean training period: 2.43±0.603 years) were randomly assigned to high intensity low-volume (HILV) and high-intensity moderate volume (HIMV) taper groups. Training frequencies were five times per week conducted for 2 weeks in both groups. At baseline and after 2 weeks of the taper intervention, psychological (TMD; characterized by the aggression, depression, tension, fatigue, confusion and vigor sub states) were measured by using self administer profiles of mood state (POMS) questionnaires. Result- We investigated the effect of the HILV and HIMV taper training on the mood disturbance of endurance athletes and positive psychological traits changes were observed in both HILV and HIMV taper groups regardless of the differences in volume reductions during the two-week taper period. Comparisons of the strategies did not reveal significant differences between the taper groups. In addition, finding from multiple regression models emphasizing on the prediction power of the tapering training strategies on the mood disturbance revealed that HILV taper could predicting the TMD in endurance athletes.Conclusion- Both taper strategies characterized by HILV and HIMV training load have beneficial effects on the improvement of endurance related psychological traits.
Application of Monitoring Indicators in The Management of Hospital Use of Antimicrobials
Authors: Silva Ravena , Silva Ravena , Santos Quesia , Batista Alessandra , Melo Matheus , Júnior Lucindo , Mendonça Simonize , Xavier Milena , Leão Ingrid , Silva Wellington , Lobo Iza Maria Fraga 
Published: May 2020

Abstract
Background It is described in the literature that between 25–50% of antimicrobial prescriptions, in the hospital environment, are unnecessary or inadequate. The National Health Surveillance Agency launched the “Brazil Stewardship Project”, in order to assess the national panorama of antimicrobial use management programs, therefore, it is expected that managers will be encouraged to implement the antimicrobial use management program, with publication of the results, enabling the confrontation of national data.Methods This is an observational, analytical and prospective study conducted in a teaching hospital, with 94 active beds, distributed between the intensive care unit (ICU), the surgical clinic (SUR), the medical clinic (MED), the pneumology/infectology (PNE/INF) and pediatrics (PED).Results During the study period, a total of 11,628 patient-days were followed up and an average of 58.3% of patients were exposed to some antimicrobial. In 376 days, for every 1,000 days of hospitalization, patients were receiving antimicrobial therapy (LOT = 376/1000pd). The calculated global DOT / LOT ratio showed that each patient received an average of 1.5 antimicrobials during hospital stay.Conclusions The indicators addressed in this study proved to be of great relevance for obtaining data regarding the use of antimicrobials, contributing to the initiatives to be taken by the Stewardship program. This study is expected to encourage other hospitals to monitor the use of antimicrobials, allowing comparison of data.
Effective Components-Targets-Mechanism-Chinese Prescription Strategy (ETMC), A New Strategy for Chinese Prescription Development: A Case Study in Danlou Tablet
Authors: Guo Yongzhi , Jiang Yingnan 
Published: May 2020

Abstract
Background: Chinese prescription is a combination medicine used by Chinese medical workers to treat various diseases and has saved countless human lives. By studying the effective substances, therapeutic targets, and mechanism of Chinese prescription, Western researchers can better understand the great value of Chinese prescription. Methods: In this study, a new strategy was proposed for the development of Chinese prescription: the effective components (E)-targets (T)-mechanism (M)-Chinese prescription (C) strategy, namely the ETMC strategy. Results: The proposed strategy used the chemical compositions of the Chinese prescription as the source of ligands to predict the corresponding targets for discovering the mechanism of Chinese prescription, which was helpful to further screen out effective substances from the chemical compositions of Chinese prescription. Here an example on development of Danlou tablet was performed to introduce the application of ETMC. Conclusions: A novel strategy for Chinese prescription development, ETMC, along with its application was provided in the current study, which contributed to the further development, wider application and internationalization of Chinese prescription.
Rhythms and prognosis of patients with cardiac arrest, emphasis on pseudo-pulseless electrical activity: another reason to use ultrasound in emergency rooms in Colombia
Authors: Devia German Alberto , Navarrete Norberto , Ortiz Zaira Melina Rojas 
Published: May 2020

Abstract
Background The cardiac arrest is still an emergency with a bad prognosis. The growing adoption of bedside ultrasound allowed to classify PEA in two groups: the true PEA and the pseudo-PEA. pPEA is used to describe a patient who has a supposed PEA in the absence of pulse, with evidence of some cardiac activity on the bedside ultrasound.Objective This work aims to assess the bedside ultrasound use as a predictor for ROSC and survival at discharge in cardiac arrest patients, and compare the pseudo-pulseless electrical activity to other cardiac arrest rhythms, including shockable rhythms.Materials and Methods This is an observational, historic cohort study carried out in the emergency room of the University Hospital Mayor, Méderi. Data were collected from all the adult patients treated for cardiac arrest from June 2018 to 2019. An ultrasound was performed to every cardiac arrest patient.Results Of a total of 108 patients the median of the age was 71 years, 65.8% were male subjects, the most frequent cause for cardiac arrest was the cardiogenic shock(32.4%). ROSC was observed in 41 cases(37.9%) and survival at discharge was 18 cases(16.7%). VF/VT and pPEA were the two rhythms that showed the highest ROSC and survival at discharge. For the pPEA group, we were able to conclude that the cardiac activity type is related to ROSC.Conclusion There is a significant difference for ROSC and survival at discharge prognosis among the cardiac arrest rhythms, with better outcomes for VF/VT and pPEA. Among patients with PEA, a routine ultrasound assessment is recommended. The type of cardiac activity recorded during the ultrasound of the cardiac arrest patient might be related to the ROSC and survival at discharge prognosis.