bluuebunny/crossref_metadata_2025_split huggingface dataset:
Traceability validation of six enzyme measurements on the Abbott Alinity c analytical system
Authors: Aloisio Elena , Frusciante Erika , Pasqualetti Sara , Infusino Ilenia , Krintus Magdalena , Sypniewska Grazyna , Panteghini Mauro 
Published: March 2020

Abstract

Background
Laboratory professionals should independently verify the correct implementation of metrological traceability of commercial measuring systems and determine if their performance is fit for purpose. We evaluated the trueness, uncertainty of measurements, and transferability of six clinically important enzyme measurements (alanine aminotransferase [ALT], alkaline phosphatase [ALP], aspartate aminotransferase [AST], creatine kinase [CK], γ-glutamyltransferase [γGT], and lactate dehydrogenase [LDH]) performed on the Abbott Alinity c analytical system.

Methods
Target values and associated uncertainties were assigned to three pools for each enzyme by using the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) reference measurement procedures (RMPs) and the pools were then measured on the Alinity system. Bias estimation and regression studies were performed, and the uncertainty associated with Alinity measurements was also estimated, using analytical performance specifications (APS) derived from biological variability of measurands as goals. Finally, to validate the transferability of the obtained results, a comparison study between two Alinity systems located in Milan, Italy, and Bydgoszcz, Poland, was carried out.

Results
Correct implementation of traceability to the IFCC RMPs and acceptable measurement uncertainty fulfilling desirable (ALP, AST, LDH) or optimal APS (ALT, CK, γGT) was verified for all evaluated enzymes. An optimal alignment between the two Alinity systems located in Milan and Bydgoszcz was also found for all enzyme measurements.

Conclusions
We confirmed that measurements of ALT, ALP, AST, CK, γGT, and LDH performed on the Alinity c analytical system are correctly standardized to the IFCC reference measurement systems and the system alignment is consistent between different platforms.
Mortalität bei Hitzeextremen in Deutschland
Authors: Uphoff Helmut , Larem Andreas , Becker Laura , Piro Ann-Kathrin , an der Heiden Matthias 
Published: March 2020

Zusammenfassung
In Deutschland werden bei zunehmender Häufigkeit von Hitzewellen bundesweit von 2001 bis 2015 fünf Sommer mit signifikanter hitzebedingter Steigerung der Mortalität erkennbar. Dabei sind insgesamt zwischen 16.000 und 37.000 zusätzliche Todesfälle durch das Robert Koch-Institut (RKI) geschätzt worden. Für Hessen wurden 2000 bis 2019 sechs Sommer mit insgesamt zwischen 1.500 bis 4.000 zusätzlichen Todesfällen geschätzt. Eine zeitnahe tagesgenaue Erfassung der Gesamtmortalität unterstützt die Fokussierung der Hitzeschutzmaßnahmen und fördert die Aufmerksamkeit und das Interesse der Medien.
Klimawandel und Gesundheit: Aktivitäten in Bayern
Authors: Kutzora Susanne , Weilnhammer Veronika , Heigl Katharina , Korbely Christine , Herr Caroline , Heinze Stefanie 
Published: March 2020

Zusammenfassung
Zur Anpassung an den Klimawandel werden in Bayern mehrere Projekte durchgeführt, die sich auf unterschiedliche klimawandelbedingte Gesundheitsherausforderungen fokussieren. Im Folgenden werden drei Projekte des Bayerischen Landesamts für Gesundheit und Lebensmittelsicherheit (LGL) zu diesem Thema vorgestellt: das Verbundprojekt „Klimawandel und Gesundheit“, das Projekt „Klimaanpassung in der Pflege“ und „ePIN – Polleninformationsnetzwerk in Bayern“.
Hitzeschutzplan Steiermark/Österreich – Klimawandelanpassung in der Praxis
Authors: Pollhammer Christian 
Published: March 2020

Zusammenfassung
Aufgrund des Klimawandels und den damit einhergehenden Herausforderungen für den öffentlichen Gesundheitsdienst wurde 2011 der Steirische Hitzeschutzplan (HSPL) vorgestellt und als operatives Maßnahmenbündel ein Hitzewarnsystem (HWS) installiert. Im Herbst 2017 wurde das HWS mittels einer Online-Umfrage evaluiert. Die Ausgestaltung eines solchen Informationssystems in der Praxis wie auch die Aufbereitung von zielgruppenrelevanten Informationen sind maßgeblich für die Effektivität und Akzeptanz von derartigen Warnsystemen.
Klimawandel und Auswirkungen auf die menschliche Gesundheit
Authors: Augustin Jobst 
Published: March 2020

Zusammenfassung
Klimatische Veränderungen haben mit hoher Wahrscheinlichkeit einen Einfluss auf die menschliche Gesundheit. Studien zeigen bereits die Auswirkungen, die zukünftig vermutlich noch an Relevanz gewinnen werden. Multikausale Zusammenhänge erschweren aber detaillierte Aussagen zu den künftigen Auswirkungen klimatischer Veränderungen auf die Gesundheit. Trotz Unsicherheiten kommt den Anpassungsmaßnahmen zur Reduzierung der Folgen des Klimawandels auf Morbidität und Mortalität eine hohe Bedeutung zu.
Hitzewellen und Klimawandel: Eine Herausforderung für Gesundheitsberufe
Authors: Schoierer Julia , Wershofen Birgit , Böse-O’Reilly Stephan , Mertes Hanna 
Published: March 2020

Zusammenfassung
Aufgrund des Klimawandels lässt sich in Deutschland bereits eine (spürbare) Steigerung von Anzahl, Dauer und Intensität von Hitzeperioden beobachten, mit Auswirkungen auf die menschliche Gesundheit, insbesondere älterer und pflegebedürftiger Personen. Das Klinikum der Universität München entwickelt Anpassungsstrategien an den Klimawandel zum Schutz der Gesundheit und in diesem Rahmen Bildungsmodule für Gesundheitsberufe sowie einen Hitzemaßnahmenplan für vollstationäre Einrichtungen der Altenpflege.
Gesundheitswesen und Klimaschutzpolitik: Synergien für eine gesunde und nachhaltige Gesellschaft
Authors: Eichinger Michael , Herrmann Martin 
Published: March 2020

Zusammenfassung
Die Überwindung der Klimakrise ist zur Schlüsselfrage für ein gesundes Leben im 21. Jahrhundert geworden. Um Synergien zwischen Gesundheitswesen und Klimaschutzpolitik zu heben, muss die Klimakrise als zentrales Querschnittsthema im Gesundheitswesen verankert werden. Zudem bedarf es der Stärkung von Lehre und Forschung, die unmittelbar zur Großen Transformation, d.h. zu einer raschen und umfassenden Neuausrichtung unserer Lebens- und Wirtschaftsweise auf Nachhaltigkeit, beitragen.
Hitze in der Stadt – Mikroklima und Anpassungsmaßnahmen: ein Fallbeispiel aus Freiburg
Authors: Matzarakis Andreas , Fröhlich Dominik 
Published: March 2020

Zusammenfassung
In der Stadt Freiburg im Breisgau im südlichen Oberrheingraben gab es um die Neugestaltung eines städtischen Platzes (Platz der Alten Synagoge) große Diskussionen. Im Vorfeld der Umgestaltung sollte mit Hilfe von Modellen die Auswirkung des Umbaus auf die in den Sommermonaten auf dem Platz ggf. auftretende Hitzebelastung für Menschen untersucht und quantifiziert werden. Die Ergebnisse der Untersuchung zeigen, wie man in Zeiten des Klimawandels Anpassungsmaßnahmen durchführen sollte.
Klimawandel und Gesundheit: Neue Herausforderungen für Public Health
Authors: Böckmann Melanie , Hornberg Claudia 
Published: March 2020

ZusammenfassungPublic Health hat vielfältige Möglichkeiten, gesundheitlichen Folgen des Klimawandels proaktiv zu begegnen. Neben Forschung zu Auswirkungen klimatischer Veränderungen, Nebeneffekten von Klimaschutz- und zur Wirksamkeit von Anpassungsmaßnahmen kommt Public Health-Expert*innen eine bedeutende Rolle in Prävention und Behandlung von klimawandelbedingten Gesundheitsstörungen zu. Erforderliches Wissen gilt es in Public Health- und Medizin-Curricula zu vermitteln. Weitere Aufgaben sind systematische Reduktionen von CO2-Emissionen im Gesundheitssektor und Umsetzung von Klimaanpassungsmaßnahmen.
Impact of age, body weight and metabolic risk factors on steroid reference intervals in men
Authors: Mezzullo Marco , Di Dalmazi Guido , Fazzini Alessia , Baccini Margherita , Repaci Andrea , Gambineri Alessandra , Vicennati Valentina , Pelusi Carla , Pagotto Uberto , Fanelli Flaminia 
Published: March 2020

Objective
To evaluate the independent impact of age, obesity and metabolic risk factors on 13 circulating steroid levels; to generate reference intervals for adult men.

Design
Cross-sectional study.

Methods
Three hundred and fifteen adults, drug-free and apparently healthy men underwent clinical and biochemical evaluation. Thirteen steroids were measured by LC-MS/MS and compared among men with increasing BMI. Moreover, the independent impact of age, BMI and metabolic parameters on steroid levels was estimated. Upper and lower reference limits were generated in steroid-specific reference sub-cohorts and compared with dysmetabolic sub-cohorts.

Results
We observed lower steroid precursors and testosterone and increase in estrone levels in men with higher BMI ranges. By multivariate analysis, 17-hydroxyprogesterone and dihydrotestosterone decreased with BMI, while cortisol decreased with waist circumference. Estrone increased with BMI and systolic blood pressure. Testosterone decreased with worsening insulin resistance. 17-hydroxypregnenolone and corticosterone decreased with increasing total/HDL-cholesterol ratio. Age-related reference intervals were estimated for 17-hydroxypregnenolone, DHEA, 17-hydroxyprogesterone, corticosterone, 11-deoxycortisol, cortisol and androstenedione, while age-independent reference intervals were estimated for progesterone, 11-deoxycorticosterone, testosterone, dihydrotestosterone, estrone and estradiol. Testosterone lower limit was 2.29 nmol/L lower (P = 0.007) in insulin resistant vs insulin sensitive men. Furthermore, the upper limits for dihydrotestosterone (−0.34 nmol/L, P = 0.045), cortisol (−87 nmol/L, P = 0.045–0.002) and corticosterone (−10.1 nmol/L, P = 0.048–0.016) were lower in overweight/obese, in abdominal obese and in dyslipidaemic subjects compared to reference sub-cohorts, respectively.

Conclusions
Obesity and mild unmedicated metabolic risk factors alter the circulating steroid profile and bias the estimation of reference limits for testosterone, dihydrotestosterone, cortisol and corticosterone. Applying age-dependent reference intervals is mandatory for steroid precursors and corticosteroids.
Coagulopathy in the Acute Phase of Traumatic Brain Injury
Authors: Baranich A. I. , Sychev A. A. , Savin I. A. , Polupan A. A. , Oshorov A. V. , Potapov A. A. 
Published: March 2020

Acute traumatic brain injury (TBI) can be accompanied by coagulopathy. In TBI, when an increased risk of hemorrhagic lesions in the brain tissue exists, keeping the normal hemostasis is crucial.The aim: to determine the incidence of coagulopathy in the acute phase of isolated TBI and identify the correlation between the types of hemostasis disorders and the severity of TBI.Materials and methods. We analyzed 323 case records of patients with TBI hospitalized to the Neurosurgery Center ICU from 2008 to 2016, 118 of them were included in the study. Criteria for inclusion were acute isolated TBI, hospitalization in the ﬁrst 72 hours after injury, young and middle-aged patients. On admission to the hospital, all patients were assessed according to the Glasgow Coma Scale (GCS) and divided into two groups: group I with severe TBI, group 2 with mild and moderate TBI. All patients underwent blood coagulation testing (APTT, PTI, ﬁbrinogen concentration, platelet count).Results. In 63.6% of patients with acute TBI coagulopathy were found, most of them (55.1%) had hypocoagulability state and were observed in the group of severe PMT (62.5%). The most frequent signs of coagulopathy were reduced PTI of less than 70% and thrombocytopenia of less than 150×109/l. A weak correlation (R=0.276; P=0.002) was found between the development of thrombocytopenia and adverse outcomes (1–3 points according to GOS).Conclusion. on hospital admission coagulopathy was diagnosed in 63,6% of patients in the acute phase of TBI, hypocoagulability state prevailed. Coagulopathy was signiﬁcantly more common in patients in the acute phase of severe TBI (GCS8 points).The mechanisms of TBI-associated coagulopathy have not yet been fully clariﬁed. Further studies of the role of platelet, plasma and ﬁbrinolytic components in the development of coagulopathy are warranted, which require more comprehensive methods of hemostasis investigation.
Smart Mode of Mechanical Lung Ventilation During Early Activation of Cardiosurgical Patients
Authors: Eremenko A. A. , Komnov R. D. 
Published: March 2020

Purpose of the study: a comparative assessment of safety and quality of respiratory support carried out using the ASV mode vs. conventional protocol, in which ventilation parameters are set by an ICU physician during early postoperative period in cardiosurgical patients.Materials and methods. The modes of a respiratory support included automated ASV ventilation (40 patients) versus conventional ventilation (38 patients) managed by 8 ICU physicians were compared in a cohort of cardiosurgical patients in a randomized controlled study.The comparison included ventilation parameters, all eﬀorts of physicians to adjust ventilator settings and time it took, duration of respiratory support in ICU, incidence of adverse events in the course of weaning, total time in ICU and hospital, postoperative complications and mortality.Results. There was no reliable diﬀerence in the duration of postoperative trachea intubation, which was equal to 267±76 minutes (the ASV group) and 271±80 minutes (the control group).The number of manual adjustments, which was 2 vs. 4 (P<0.00001), and the time spent by a clinical physician near a ventilator, which was 99±35 seconds vs. 166±70 seconds, were reliably lower in the ASV group (P=0.00001).The time between restoration of patient’s own respiratory activity and transfer to the assisted breathing mode was longer in the control group and amounted to 30 (0–90) min. while in the smart mode, the transfer took place immediately after restoration (P=0.004969).When ASV was used, the driving pressure was reliably lower during all phases of respiratory support: ΔP 7.2±1.6 vs. 9.3±2.1 cm H2O, (P=0.000001); there was no reliable diﬀerence in the tidal volume: 7.0 (6–8.5) (ASV) vs. 7 (6–10) ml/kg/ideal body mass (the control group).Conclusion. ASV represents a lung-protective ventilation that reduces physician’s time cost and medical staﬀ eﬀorts in ALV management without compromising patient’s safety and respiratory support quality.
Models of Ischemic Stroke (Review)
Authors: Golubev A. M. 
Published: March 2020

The incidence of ischemic stroke remains high in many countries, despite a declining trend in the incidence of brain circulation disorders. Limited knowledge of the pathogenesis, diagnosis, clinical presentation, and treatment of this life-threatening disease can be complemented by modelling of ischemic stroke on animals, particularly, in rodents (rats, mice).The aim of review: to provide a rationale for choosing an optimal model of human ischemic stroke.Among more than 300 primary literature sources from various databases (Scopus, Web of science, RSCI, etc.), 84 sources were selected for evaluation, of which 72 were published in the recent years (2015–2019). The criteria for exclusion of sources were low relevance and outdated data.The review examined six experimental models of acute cerebrovascular events including middle cerebral artery occlusion, embolic stroke model; phototrombosis; models using thrombin, endothelin-1 and electrocoagulation of the middle cerebral artery.The review outlines the factors inﬂuencing reliability and reproducibility of research results, related to adherence to the rules of animal accommodation and acclimatization, nutrition and care, selection of anesthesia and pain relief methods, compliance with aseptic techniques, monitoring of basic physiological parameters at all stages of the ischemic stroke modeling experiment, with humane withdrawal of animals from the experiment.It was concluded that the model of ischemic stroke, based on the occlusion of the middle cerebral artery, is most promising as being the closest to features of human ischemic stroke and enabling to obtain reproducible results in the experiment.
AN EFFECTIVE OF POGIL WITH VIRTUAL LABORATORY IN IMPROVING SCIENCE PROCESS SKILLS AND ATTITUDES: SIMPLE HARMONIC MOTION CONCEPT
Authors: Alatas Fathiah , Fachrunisa Ziah 
Published: March 2020

EFEKTIVITAS POGIL DENGAN LABORATORIUM VIRTUAL DALAM PROSES MENINGKATKAN KETERAMPILAN DAN SIKAP ILMU PENGETAHUAN: KONSEP MOTION MOTION SIMPLE HARMONIC AbstractSimple harmonic motion is a concept that often involve complex and difficult equations, so that students can’t construct knowledge of the concept. This research applies POGIL with a virtual laboratory, where students are encouraged to construct the concept and emphasize collaborative learning with different roles. Virtual laboratory allows students to explore and establish students’ science process skills and attitude. This study used a quasi experiment with nonequivalent control group design. Therefore, this study has add to the practical knowledge to develop science process skills and establish a positive attitudes of students toward science.AbstrakGerak harmonik sederhana merupakan konsep yang sering melibatkan persamaan yang rumit dan dianggap sulit, sehingga siswa tidak dapat membangun pengetahuan terhadap konsep. Penelitian ini menerapkan model POGIL dengan virtual laboratorium, dimana siswa didorong untuk mengkontruksi konsep dan menekankan belajar kolaboratif dengan peran berbeda. Virtual laboratorium memudahkan siswa untuk bereksplorasi. Ini menumbuhkan keterampilan proses sains serta sikap ilmiah siswa. Metode penelitian yang digunakan adalah quasi eksperiment dengan desain nonequivalent control group. Oleh karena itu, penelitian ini menambah pengetahuan praktis untuk mengembangkan keterampilan proses sains dan menumbuhkan sikap positif siswa terhadap sains.
DEVELOPING METACOGNITIVE SKILL INSTRUMENT ON FUNGUS CONCEPT
Authors: Zulfiani Zulfiani , Herlanti Yanti , Rosydatun Eny S. , Hasiani Silak , Rohmatulloh Ghina , Zuqistya Nila 
Published: March 2020

PENGEMBANGAN INSTRUMEN KETERAMPILAN METAKOGNITIF PADA KONSEP JAMURAbstractMetacognition involves critical thinking skills, reflective of the cognitive processes of learners who simultaneously monitor their learning success. This research is aimed to develop metacognitive skill instrument on the mushroom concept of class X students of SMA Negeri in South Tangerang. Development research method / Formative Research from van den Akker (2006) with cycle/spiral process: analysis, design, evaluation and revision, systematic reflection and documentation. The metacognitive skills instrument consists of 12 questions, with the construction of 3 high order thinking skill, open-ended, cognitive thinking questions with 9 questions of metacognitive skills (planning, monitoring, and evaluation). Instruments validated through expert judgment and trial test were limited to 38 students and obtained Cronbach Alpha value of 0.832 (high criterion). The instrument was then revised and re-tested on 96 learners and obtained by Alpha Cronbach 0.746 (high category). The results of this study acquired an alternative method of metacognitive skills measurement of learners are integrated into the biology of learning in the classroom.AbstrakMetakogisi melibatkan keterampilan berpikir kritis, reflektif terhadap proses kognitif peserta didik yang secara simultan memantau keberhasilan belajarnya. Penelitian ini bertujuan mengembangkan instrumen keterampilan metakognitif pada konsep Jamur peserta didik kelas X SMA Negeri di Tangerang Selatan. Metode penelitian pengembangann atau development research dari van den Akker (2006) dengan proses siklus/spiral: analisis, desain, evaluasi dan revisi, refleki sistematik dan dokumentasi. Instrumen keterampilan metakognitif berjumlah 12 pertanyaan, dengan konstruksi 3 pertanyaan kognitif berpikir tingkat tinggi, open ended disertai 9 pertanyaan keterampilan metakognitif (perencanaan, monitoring, dan evaluasi). Instrumen divalidasi melalui expert judgment dan uji coba terbatas pada 38 peserta didik dan diperoleh nilai reliabilitas Alpha Cronbach 0.832 (kategori tinggi). Instrumen kemudian direvisi dan diuji coba kembali pada 96 peserta didik dan diperoleh nilai Alpha Cronbach 0.746 (kategori tinggi). Hasil penelitian ini diperoleh alternatif metode pengukuran keterampilan metakognitif peserta didik yang terintegrasi dalam pembelajaran biologi di kelas.
THE DEVELOPMENT OF INTEGRATED SCIENCE PRACTICE ON SKIN HEALTH THEME ‎TO ENHANCE THE SCIENCE PROCESS SKILL OF JUNIOR HIGH SCHOOL STUDENTS ‎
Authors: Purnamasari Shinta , Surtikanti Hertien 
Published: March 2020

Science learning have to able to provide direct experiences to the students, so they not only learn the concepts and theories, but also can learn science as a process through lab activity. In middle school, science learning conducted in integrated mode in order to provide opportunities for students to understand the phenomenon from various sides. The aim of this study was to produce an experiment procedure to teach integrated science in skin health theme that can be implement it in learning process. A hands-on activity is presented in worksheet form. This study used Research and Development method. The stages were conducted in this study consist of preliminary studies and model development. Data collected through experts assessment sheet, and readability questionnaire and science process skills test. Based on experts assessment sheet obtained that experiment procedure has a good quality with average percentage about 90.56% which includes didactic aspect (93.21%), construction aspect (88.89%), and technical aspect (91.67%). An experiment procedure was presented in worksheet form andhas a very good readability level with average percentage about 82.80%. The test result showed that an experiment procedure can enhance students’ science process skills especially in doing an experiment aspect. Keywords: experiment development; integrated science experiment; skin health; sunscreen.
Brave New Digital Classroom: Technology and Foreign Language Learning (Third Edition), by Robert J. Blake and Gabriel Guillén
Authors: Colpaert Jozef 
Published: March 2020

Brave New Digital Classroom: Technology and Foreign Language Learning (Third Edition) Robert J. Blake and Gabriel Guillén Washington, DC: Georgetown University Press US $32.95 ISBN: 9781626167407 (Paperback) 248 pages 2020
Enhancing Signal-to-Noise Ratios of High-Frequency Rayleigh Waves Extracted from Ambient Seismic Noises in Topographic Region
Authors: Ping Ping , Chu Risheng , Zhang Yu , Xie Jun 
Published: March 2020

ABSTRACTHigh-frequency Rayleigh waves can be extracted from ambient seismic noises through noise correlation functions (NCFs), which provides a useful tool to image shallow structures in topographic regions, for example, landslides. Topography may affect signal-to-noise ratios (SNRs) of extracted Rayleigh waves. It is necessary to investigate the propagation features of Rayleigh waves passing a 3D topography. Based on the incident and scattered waves satisfying the free surface boundary conditions, we first derive the displacement responses of Rayleigh waves across a 3D elastic wedge. The results show that the particle motions of Rayleigh waves are an ellipse whose longer axis is always perpendicular to the topographic free surface. Therefore, the Qg component, perpendicular to the topographic free surface, is a better choice to extract high-frequency Rayleigh waves than the conventional vertical component. To verify the choice, we carry out numerical simulations to extract high-frequency NCFs for a typical 3D massif model. Finally, we apply this approach to extract high-frequency Rayleigh-wave NCFs on the Xishancun landslide in southwestern China. The NCFs obtained using the Qg component have more coherent waveforms and higher SNRs than those using the vertical component. We conclude that the Qg component has advantages in extracting high-frequency Rayleigh waves over the conventional vertical component.