bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accurate bidiagonal decomposition of collocation matrices of weighted ϕ‐transformed systems
Authors: Mainar Esmeralda , Peña Juan Manuel , Rubio Beatriz 
Published: March 2020

SummaryGiven a system of functions, we introduce the concept of weighted φ‐transformed system, which will include a very large class of useful representations in Statistics and Computer Aided Geometric Design. An accurate bidiagonal decomposition of the collocation matrices of these systems is obtained. This decomposition is used to present computational methods with high relative accuracy for solving algebraic problems with collocation matrices of weighted φ‐transformed systems such as the computation of eigenvalues, singular values, and the solution of some linear systems. Numerical examples illustrate the accuracy of the performed computations.
Impact of Medicaid expansion on access and healthcare among individuals with sickle cell disease
Authors: Kayle Mariam , Valle Jhaqueline , Paulukonis Susan , Holl Jane L. , Tanabe Paula , French Dustin D. , Garg Ravi , Liem Robert I. , Badawy Sherif M. , Treadwell Marsha J. 
Published: March 2020

AbstractPurposeSickle cell disease (SCD) is associated with high acute healthcare utilization. The purpose of this study was to examine whether Medicaid expansion in California increased Medicaid enrollment, increased hydroxyurea prescriptions filled, and decreased acute healthcare utilization in SCD.MethodsIndividuals with SCD (≤65 years and enrolled in Medicaid for ≥6 total calendar months any year between 2011 and 2016) were identified in a multisource database maintained by the California Sickle Cell Data Collection Program. We describe trends and changes in Medicaid enrollment, hydroxyurea prescriptions filled, and emergency department (ED) visits and hospital admissions before (2011‐2013) and after (2014‐2016) Medicaid expansion in California.ResultsThe cohort included 3635 individuals. Enrollment was highest in 2014 and lowest in 2016 with a 2.8% annual decease postexpansion. Although <20% of the cohort had a hydroxyurea prescription filled, the percentage increased by 5.2% annually after 2014. The ED visit rate was highest in 2014 and decreased slightly in 2016, decreasing by 1.1% annually postexpansion. Hospital admission rates were similar during the pre‐ and postexpansion periods. Young adults and adults had higher ED and hospital admission rates than children and adolescents.ConclusionsMedicaid expansion does not appear to have improved enrollment or acute healthcare utilization among individuals with SCD in California. Future studies should explore whether individuals with SCD transitioned to other insurance plans or became uninsured postexpansion, the underlying reasons for low hydroxyurea utilization, and the lack of effect on hospital admissions despite a modest effect on ED visits.
Immunochemical approaches for detection of aflatoxin B1 in herbal medicines
Authors: Raysyan Anna , Eremin Sergei A. , Beloglazova Natalia V. , De Saeger Sarah , Gravel Irina V. 
Published: March 2020

AbstractIntroductionAflatoxin B1 (AFB1) is a toxic low‐molecular‐weight secondary metabolite of Aspergillus flavus and A. parasiticus. AFB1 was classified as a Group I carcinogen by the World Health Organisation for Research on Cancer in 1993. AFB1 is an unavoidable natural contaminant of some herbal medicine, able to cause serious health issues for humans consuming the related medicine.ObjectiveTherefore, this study aimed to develop an efficient fluorescence polarisation immunoassay (FPIA) and a rapid, low‐cost, and easy‐to‐use membrane‐based flow‐through immunoassay (MBA) for determination of AFB1 in herbal medicine Origanum vulgare L., Rubus idaeus L., Urtica dioica L. and Sorbus aucuparia L.ResultsA cut‐off level of the developed MBA was 0.8 ppb. Validation of the developed test was performed with blank and spiked samples. Using three naturally contaminated or three artificially spiked samples. The FPIA showed a linear working range of 8.6 to 64 ppb, and a half maximal inhibitory concentration (IC50) of 24 ppb.ConclusionThe results were in good correlation with the enzymelinked immunosorbent assay (ELISA) results (the IC50 0.1 ppb). Both the sample preparation and analysis are simple, cost‐effective and easy to perform on‐site in non‐laboratory environments. Liquid chromatography with tandem mass spectrometry (LC‐MS/MS) was used as a confirmatory technique.
Comparison of Hydromorphone versus Fentanyl‐based Sedation in Extracorporeal Membrane Oxygenation: A Propensity‐Matched Analysis
Authors: Landolf Kaitlin M. , Rivosecchi Ryan M. , Goméz Hernando , Sciortino Christopher M. , Murray Holt N. , Padmanabhan Rajagopala R. , Sanchez Pablo G. , Harano Takashi , Sappington Penny L. 
Published: March 2020

IntroductionData comparing sedatives in patients receiving extracorporeal membrane oxygenation (ECMO) are sparse. However, it is known that the ECMO circuit alters the pharmacokinetic properties of medications via drug sequestration of lipophilic agents and increased volume of distribution.ObjectivesThis study evaluated the difference in days alive without delirium or coma and the sedative requirements in patients receiving fentanyl versus hydromorphone in ECMO patients.MethodsThis single‐center retrospective observational study evaluated adults receiving ECMO for more than 48 hours and continuous infusion of either fentanyl or hydromorphone for at least 6 hours. Of 148 patients evaluated, 88 received fentanyl and 60 received hydromorphone continuous infusion sedation. Outcomes included delirium‐free and coma‐free (DFCF) days, narcotic use, and sedative use.Main ResultsThere was an increase in the number of DFCF days in the hydromorphone group at day 7 (p=0.07) and day 14 (p=0.08) and a significant reduction in daily fentanyl equivalent exposure. Propensity score matching yielded 54 matched pairs. An 11.1% increase was observed in the proportion of ECMO days alive without delirium or coma in the hydromorphone group at 7 days (53.2% vs 42.1%, p=0.006). Patients in the hydromorphone group received significantly fewer narcotics with a median of 555 µg (interquartile range [IQR] 287–905 µg) of fentanyl equivalents per day compared with 2291 µg (IQR 1053–4023 µg) in the fentanyl group (p<0.005).ConclusionThe use of hydromorphone‐based sedation in ECMO patients resulted in more days alive without delirium or coma while significantly reducing narcotic requirements.
Site‐specific recombinase genome engineering toolkit in maize
Authors: Cody Jon P. , Graham Nathaniel D. , Zhao Changzeng , Swyers Nathan C. , Birchler James A. 
Published: March 2020

AbstractSite‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, reactions result in integrations, excisions, or inversions of targeted DNA sequences in a precise and predictable manner. Here, we established five different stable recombinase expression lines in maize through Agrobacterium‐mediated transformation of T‐DNA molecules that contain coding sequences for Cre, R, FLPe, phiC31 Integrase, and phiC31 excisionase. Through the bombardment of recombinase activated DsRed transient expression constructs, we have determined that all five recombinases are functional in maize plants. These recombinase expression lines could be utilized for a variety of genetic engineering applications, including selectable marker removal, targeted transgene integration into predetermined locations, and gene stacking.
Model‐Informed Drug Development for Everolimus Dosing Selection in Pediatric Infant Patients
Authors: Combes Francois Pierre , Einolf Heidi J. , Coello Neva , Heimbach Tycho , He Handan , Grosch Kai 
Published: March 2020

Everolimus is currently approved in Europe as an adjunctive therapy for patients aged ≥ 2 years with tuberous sclerosis complex (TSC)–associated treatment‐refractory partial‐onset seizures, based on the EXIST‐3 study (NCT01713946) results. As TSC‐associated seizures can also affect children aged between 6 months and 2 years, a modeling and simulation (M&S) approach was undertaken to extrapolate exposure (trough plasma concentration (Cmin)) after a dose of 6 mg/m2 and reduction in seizure frequency (RSF). A physiologically based pharmacokinetic model using Simcyp was developed to predict Cmin in adult and pediatric patients, which was then used by a population pharmacodynamic model and a linear mixed effect model to predict short‐term and long‐term efficacy in adults (for validation) and in children, respectively. Based on the results of the M&S study, everolimus at the dose of 6 mg/m2 is anticipated to be an efficacious treatment in children 6 months to 2 years of age (up to 77.8% RSF) with concentrations within the recommended target range.
Biological Interfaces, Modulation, and Sensing with Inorganic Nano‐Bioelectronic Materials
Authors: Schaumann Erik N. , Tian Bozhi 
Published: March 2020

AbstractThe last several years have seen a large and increasing interest in scientific developments that combine methods and materials from nanotechnology with questions and applications in bioelectronics. This follows with a number of broader trends: the rapid increase in functionality for materials at the nanoscale; a growing recognition of the importance of electric fields in diverse physiological processes; and continuous improvements in technologies that are naturally complementary with bioelectronics, such as optogenetics. Here, a progress report is provided on several of the most exciting recent developments in this field. The three critical functions of biointerface formation, biological modulation, and biological sensing using newly developed nanoscale materials are considered.
Neurodevelopmental outcome at 2 years of corrected age in fetuses with increased nuchal translucency thickness and normal karyotype compared with matched controls
Authors: Buffin R. , Fichez A. , Decullier E. , Roux A. , Bin S. , Combourieu D. , Pastor‐Diez B. , Huissoud C. , Picaud J.‐C. ,   collaborating authors
Published: March 2020

ABSTRACTObjectivesIncreased nuchal translucency (NT) thickness is an antenatal marker of aneuploidy or malformation that can lead to termination of pregnancy. This study assessed the long‐term neurodevelopmental prognosis of infants who had isolated increased NT in utero.MethodsThis was a prospective cohort study of infants with a NT thickness > 95th percentile in the first trimester, but with a normal karyotype and no major anomalies, and controls with normal NT matched for birth weight, Apgar score, place of birth, parity and gestational age at birth. At 2 years of corrected age, all infants underwent the psychometric Brunet–Lézine test to evaluate their developmental quotient (DQ), overall (global) and specifically for the areas of posture, language, coordination and sociability.ResultsA total of 203 chromosomally normal infants were included in the increased‐NT group and 208 in the control group. The mean global DQ was significantly lower in the increased‐NT group than in the control group (108.6 ± 9.7 vs 112.8 ± 8.3; P < 0.0001), but it was within the normal range expected for that age in both groups. Similarly, the mean DQs for coordination, sociability and language, but not for posture, were significantly lower in infants with increased NT than in controls. Only one case with increased NT had a DQ < 70 (defined as severe neurodevelopmental impairment), compared with none in the control group. The difference between the two groups remained significant for a NT threshold ≥ 99th percentile and when the data were adjusted for NT thickness, the infant's sex and the mother's educational level. In the increased‐NT group, NT thickness was < 3.5 mm in over half (56%) of the infants, between 3.5 mm and 5 mm in 33% and > 5 mm in 11%, with a mean global DQ of 108.4, 110.1 and 109.7, respectively.ConclusionsInfants who had isolated increased fetal NT in the first trimester had a significantly lower, but normal, DQ at a corrected age of 2 years, when compared with controls. The findings were independent of the infant's sex, fetal NT thickness and the mother's educational level. © 2020 Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.
Flavor structure of magnetized T2/ℤ2 blow-up models
Authors: Kobayashi Tatsuo , Otsuka Hajime , Uchida Hikaru 
Published: March 2020

Abstract
We discuss the flavor structure in resolution of magnetized T2/ℤ2 orbifold models. The flavor structure of Yukawa couplings among chiral zero-modes is sensitive to radii of the resolved fixed points of T2/ℤ2 orbifold. We show in concrete models that the realistic mass hierarchy and mixing angles of the quark sector can be realized at certain values of blow-up radii, where the number of parameters in the blow-up model can be smaller than toroidal orbifold one.
Significant association between tumor mutational burden and immune-related adverse events during immune checkpoint inhibition therapies
Authors: Kerepesi Csaba , Bakacs Tibor , Moss Ralph W. , Slavin Shimon , Anderson Colin C. 
Published: March 2020

AbstractMore than 2000 immuno-oncology agents are being tested or are in use as a result of the cancer immunotherapy revolution. Manipulation of co-inhibitory receptors has achieved tumor eradication in a minority of patients, but widespread immune-related adverse events (irAEs) compromised tolerance to healthy self-tissues in the majority. We have proposed that a major mechanism of irAEs is similar to a graft-versus-malignancy effect of graft-versus-host disease. To verify our hypothesis, we retrieved post-marketing data of adverse events from the U.S. Food and Drug Administration Adverse Event Reporting System. A significant positive correlation was revealed in 7677 patients between the reporting odds ratio of irAEs during immune checkpoint inhibitor therapy and the corresponding tumor mutational burden across 19 cancer types. These results can be interpreted to mean that the ICI drugs unleashed T cells against “altered-self,” self, and tumors resulting in better overall survival.
Adsorption process for phospholipids of different chain lengths at a fluorocarbon/water interface studied by Du Noüy ring and spinning drop
Authors: Ullmann Kirsten , Poggemann Lukas , Nirschl Hermann , Leneweit Gero 
Published: March 2020

AbstractFluorocarbons are novel systems in the fast-growing fields of diverse biomedical applications and fluorocarbon-water emulsions. However, characterization of these systems with modern measuring techniques such as drop profile analysis tensiometry is almost impossible because of practically identical refractive indexes and high-density differences. Due to the material properties of the fluorocarbon-water system, the invasive Du Noüy ring is the most appropriate method to measure interfacial tensions over long times. However, the influence of the ring on a fluorocarbon/water interface packed with phospholipids needs careful analysis. For the proof of methodology, the spinning drop tensiometry was used for comparison as a non-invasive technique to measure interfacial tension between water and perfluoroperhydrophenanthrene (PFPH) covered by 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) proving almost identical results. This demonstrates the validity of the invasive measurement technique for the studied system. The Du Noüy ring method was applied for further measurements of phospholipids with different chain lengths (1,2-dmyristoyl-sn-glycero-3-phostphatidylcholine, DMPC; 1,2-distearoyl-sn-glycero-3-phosphatidylcholine, DSPC) which revealed a difference in interfacial adsorption kinetics and equilibrium tensions. The Du Noüy ring tensiometry is appropriate to examine the slow adsorption kinetics of phospholipids emulsifying fluorocarbons. The results enable functional optimization of fluorocarbon emulsions regarding physical emulsification parameters and the selection of lipids.
Seasonal effect on the incidence of post-operative wound complications after trauma-related surgery of the foot, ankle and lower leg
Authors: Sanders Fay Ruth Katharina , van’t Hul Mirjam , Kistemaker Rosanne Maria Güzelleke , Schepers Tim 
Published: March 2020

Abstract
Introduction
Post-operative wound complications remain among the most common complications of orthopedic (trauma) surgery. Recently, studies have suggested environmental factors such as season to be of influence on wound complications. Patients operated in summer are reported to have more wound complications, compared to other seasons. The aim of this study was to identify if “seasonality” was a significant predictor for wound complications in this cohort of trauma-related foot/ankle procedures.

Materials and methods
This retrospective cohort study included all patients undergoing trauma-related surgery (e.g. fracture fixation, arthrodesis, implant removal) of the foot, ankle or lower leg. Procedures were performed at a Level 1 Trauma Center between September 2015 until March 2019. Potential risk factors/confounders were identified using univariate analysis. Procedures were divided into two groups: (1) performed in summer (June, July or August), (2) other seasons (September–May). The number of surgical wound complications (FRIs, SSIs or wound dehiscence) was compared between the two groups, corrected for confounders, using multivariate regression.

Results
A total of 599 procedures were included, mostly performed in the hindfoot (47.6%). Patients were on average 46 years old, and mostly male (60.8%). The total number of wound complications was 43 (7.2%). Age, alcohol abuse, open fracture and no tourniquet use were independent predicting factors. No difference in wound complications was found between summer and other seasons, neither in univariate analysis [4 (3.2%) vs 39 (8.2%), p = 0.086] nor when corrected for predicting factors as confounders (p = 0.096).

Conclusions
No seasonality could be identified in the rate of wound complications after trauma surgery of the lower leg, ankle and foot in this cohort. This lack of effect might result from the temperate climate of this cohort. Larger temperature and precipitation differences may influence wound complications to a larger extent. However, previous studies suggesting seasonality in wound complications might also be based on coincidence.
The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism
Authors: Sun Yanhua , Li Ming , Liu Guangjie , Zhang Xue , Zhi Lianghui , Zhao Jing , Wang Guiying 
Published: March 2020

Abstract
Objective
Increasing evidence has revealed that mechanical stress and elevated mechanical signals promote malignant tumor transformation and metastasis. This study aimed to explore the function of the mechanically activated ion-channel Piezo1 in the colon cancer metastasis and its potential regulatory mechanism.

Methods
First, we examined the expression levels of Piezo1 and mitochondrial calcium uniporter (MCU) both in colon cancer tissues and assessed the prognostic value of Piezo1 and MCU in a colon cancer cohort (n = 110). Second, functional assays were performed to investigate the effects of Piezo1 and MCU on colon cancer cell migration, invasion, and mitochondrial membrane potential. Third, we analyzed the expression of Piezo1, MCU, and HIF-1α by overexpressing/silencing each other’s expression.

Results
We found that Piezo1 was up-regulated and MCU was down-regulated in colon cancer tissues. Piezo1 and MCU were both correlated with poor prognosis of patients with colon cancer. Overexpressing Piezo1 and silencing MCU could promote colon cancer cell migration and metastasis, reduce mitochondrial membrane potential, and promote each other’s expression. We also found that HIF-1α was up-regulated in colon cancer tissues. Additionally, silencing Piezo1 inhibited the expression of HIF-1α and VEGF, which was contrary to MCU silencing. Intriguingly, Piezo1-overexpressing cells did not regain their migration behaviors when HIF-1α expression was inhibited, which was accompanied with the re-expression of MCU and VEGF.

Conclusion
In our study, Piezo1 is involved in colon cancer cell metastasis. Furthermore, our findings indicated a possible Piezo1-MCU-HIF-1α-VEGF axis, which still need further exploration.
Interventions to Improve Antiretroviral Therapy Adherence Among Adolescents and Youth in Low- and Middle-Income Countries: A Systematic Review 2015–2019
Authors: Reif Lindsey K. , Abrams Elaine J. , Arpadi Stephen , Elul Batya , McNairy Margaret L. , Fitzgerald Daniel W. , Kuhn Louise 
Published: March 2020

AbstractAdolescents and youth living with HIV have poorer antiretroviral treatment (ART) adherence and viral suppression outcomes than all other age groups. Effective interventions promoting adherence are urgently needed. We reviewed and synthesized recent literature on interventions to improve ART adherence among this vulnerable population. We focus on studies conducted in low- and middle-income countries (LMIC) where the adolescent and youth HIV burden is greatest. Articles published between September 2015 and January 2019 were identified through PubMed. Inclusion criteria were: [1] included participants ages 10–24 years; [2] assessed the efficacy of an intervention to improve ART adherence; [3] reported an ART adherence measurement or viral load; [4] conducted in a LMIC. Articles were reviewed for study population characteristics, intervention type, study design, outcomes measured, and intervention effect. Strength of each study’s evidence was evaluated according to an adapted World Health Organization GRADE system. Articles meeting all inclusion criteria except being conducted in an LMIC were reviewed for results and potential transportability to a LMIC setting. Of 108 articles identified, 7 met criteria for inclusion. Three evaluated patient-level interventions and four evaluated health services interventions. Of the patient-level interventions, two were experimental designs and one was a retrospective cohort study. None of these interventions improved ART adherence or viral suppression. Of the four health services interventions, two targeted stable patients and reduced the amount of time spent in the clinic or grouped patients together for bi-monthly meetings, and two targeted patients newly diagnosed with HIV or not yet deemed clinically stable and augmented clinical care with home-based case-management. The two studies targeting stable patients used retrospective cohort designs and found that adolescents and youth were less likely to maintain viral suppression than children or adults. The two studies targeting patients not yet deemed clinically stable included one experimental and one retrospective cohort design and showed improved ART adherence and viral suppression outcomes. ART adherence and viral suppression outcomes remain a major challenge among adolescents and youth. Intensive home-based case management models of care hold promise for improving outcomes in this population and warrant further research.