bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ovum pick‐up and in vitro maturation in spotted paca (Cuniculus paca—Linnaeus, 1766)
Authors: Barros Felipe Farias Pereira da Câmara , Teixeira Pedro Paulo Maia , Padilha‐Nakaghi Luciana Cristina , Uscategui Ricardo Andres Ramirez , Lima Marina Ragagnin , Santos Victor José Correia , Rossy Kayan da Cunha , Borges Luísa Pucci Bueno , Machado Marcia Rita Fernandes , Vicente Wilter Ricardo Russiano 
Published: January 2020

AbstractWe tested FSHp, eCG and FSHp + eCG to establish ovum pick‐up (OPU) and in vitro maturation method in spotted paca. Eight healthy adult females were subjected to each of four treatments to stimulate ovarian follicular growth. All females were subjected to a hormonal protocol using a single dose of 45 mg of injectable progesterone and single intramuscular injection of 0.075 mg d‐cloprostenol on day 6. Ovarian stimulation was carried out as follows: in Group TFE (FSHp and eCG), animals were treated with a single dose of 80 mg of FSHp and 200 IU of eCG intramuscularly on day 6 after the application of progesterone; in Group TF (FSHp), they were treated with a single dose of 80 mg of FSHp intramuscularly on day 6 after application of progesterone; in Group treatment eCG, they were treated with 200 IU of eCG intramuscularly on day 6 after application of progesterone; and in Group TC (saline solution), 1 ml of saline solution was administered to control does. The OPU was performed between 22 and 26 hr after gonadotropin treatments. All recovered oocytes were placed into maturation media and incubated for 24 hr. There were no differences among the mean number of observed follicles, aspirated follicles and oocytes recovered per treatment. Oocyte maturation rates did not differ among groups, except, TF and treatment eCG oocytes had greater maturation rates than TC oocytes. In this study, gonadotropin administration failed to superovulate treated does and increase oocyte retrieval efficiency. Despite the feasibility of the procedure, further studies are needed to develop and refine hormonal protocols for oocyte recovery and in vitro maturation in this species.
Can Testimony Transmit Understanding?
Authors: Malfatti Federica I. 
Published: January 2020

AbstractCan we transmit understanding via testimony in more or less the same way in which we transmit knowledge? The standard view in social epistemology has a straightforward answer: no, we cannot. Three arguments supporting the standard view have been formulated so far. The first appeals to the claim that gaining understanding requires a greater cognitive effort than acquiring testimonial knowledge does. The second appeals to a certain type of epistemic trust that is supposedly characteristic of knowledge transmission (and maybe of the transmission of epistemic goods in general) and that is allegedly incompatible with understanding. The third aims to show that there is a certain aspect of understanding (what epistemologists these days like to call “grasping”) that cannot be passed on to another person via testimony alone. In this article, I show that all of these arguments can be resisted. Thus, there seem to be no compelling reasons to embrace the standard view.
Prognostic value of colonic and peripheral venous lactate measurements in horses with large colon volvulus
Authors: Orr Kindra. E. , Baker W. True , Lynch Tim M. , Hughes Faith E. , Clark Carol K. , Slone Donnie E. , Fogle Callie A. , Gonzalez Liara M. 
Published: January 2020

AbstractObjectiveTo determine the prognostic value of (1) colonic venous lactate or peripheral lactate values obtained before and after manual correction of a large colon volvulus and (2) a combination of variables including pelvic flexure biopsy.Study designProspective clinical study.AnimalsForty adult horses in which large colon volvulus was diagnosed intraoperatively.MethodsColonic venous, peripheral venous, and arterial blood samples were collected to measure lactate values before and after manual correction. Mucosal biopsy samples were obtained in cases that underwent enterotomy or colonic resection and anastomosis. Interstitium to crypt (I:C) ratio and hemorrhage scores were measured. Optimal cutoff values were determined by receiver operator curve analysis, and associations between variables and short‐term outcome were determined by univariable regression. Short‐term survival was defined as horses being discharged from the hospital.P ≤ .05 was considered significant.ResultsNo association was found between colonic venous lactate values before (P= .011) or after (P= .201) manual correction of large colon volvulus and determination of short‐term outcome. Peripheral venous lactate at admission ≥3.2 mmol/L and after manual correction ≥5 mmol/L, arterial lactate postmanual correction ≥3.53 mmol/L, and histomorphometric measurements of mucosal hemorrhage ≥3 and I:C ratio > 1 were associated with poor short‐term outcome.ConclusionPeripheral lactate values, histomorphometric measures of I:C ratio, and hemorrhage score provided prognostic information that could help guide recommendations made to owners.Clinical significancePeripheral lactate values after manual correction provide important intraoperative diagnostic information to assist in predicting case outcome in the operative and immediately postoperative period.
Preliminary evaluation of an osteochondral autograft, a prosthetic implant, and a biphasic absorbable implant for osteochondral reconstruction in a sheep model
Authors: Olive Mélanie , Boyer Cécile , Lesoeur Julie , Thorin Chantal , Weiss Pierre , Fusellier Marion , Gauthier Olivier 
Published: January 2020

AbstractObjectiveTo determine the ability of three implants to enhance the healing of osteochondral defects: (1) a biphasic construct composed of calcium phosphate (CaP) and chitosan/cellulosic polymer, (2) a titanium‐polyurethane implant, and (3) an osteochondral autograft.Study designExperimental study.AnimalsTen adult female sheep.MethodsIn five sheep, an 8‐mm diameter osteochondral defect was created on the medial femoral condyle of a stifle and filled with a synthetic titanium‐polyurethane implant. In five sheep, a similar defect was filled with an osteochondral autograft, and the donor site was filled with a biphasic construct combining CaP granules and a chitosan/cellulosic polymer. Sheep were monitored daily for lameness. Stifle radiographs and MRI were evaluated at 20 weeks, prior to animals being humanely killed. Surgical sites were evaluated with histology, microcomputed tomography, and scanning electron microscopy.ResultsClinical outcomes were satisfactory regardless of the tested biomaterials. All implants appeared in place on imaging studies. Osteointegration of prosthetic implants varied between sites, with limited ingrowth of new bone into the titanium structure. Autografts and biphasic constructs were consistently well integrated in subchondral bone. All autografts except one contained a cartilage surface, and all biphasic constructs except one partially restored hyaline cartilage surface.ConclusionBiphasic constructs supported hyaline cartilage and subchondral bone regeneration, although restoration of the articular cartilage was incomplete.Clinical impactBiphasic constructs may provide an alternative treatment for osteochondral defects, offering a less invasive approach compared with autologous grafts and eliminating the requirement for a prosthetic implant.
Circulating miRNAs are associated with sleep duration in children/adolescents: Results of the I.Family Study
Authors: Iacomino Giuseppe , Lauria Fabio , Russo Paola , Marena Pasquale , Venezia Antonella , Iannaccone Nunzia , De Henauw Stefaan , Foraita Ronja , Heidinger‐Felső Regina , Hunsberger Monica , Kourides Yiannis , Moreno Luis A , Thumann Barbara , Veidebaum Toomas , Siani Alfonso ,   on behalf of the IDEFICS/I.Family consortium
Published: January 2020

New Findings
What is the central question of this study?
Are differential patterns of circulating miRNAs associated with sleep duration in normal‐weight European children and adolescents?

What is the main finding and its importance?
Differences in the expression level of circulating miR‐26b‐3p and miR‐485‐5p are positively associated with total sleep duration in healthy normal‐weight children and adolescents.

AbstractIt is commonly recognized that sleep is essential for children's health, and that insufficient sleep duration is associated with negative health outcomes. In humans, sleep duration and quality are influenced by genetic, environmental and social factors. Epigenetic mechanisms, likewise, regulate circadian rhythms and sleep patterns. In the present study, we aimed to identify circulating microRNAs associated with sleep duration in a subsample of normal‐weight European children/adolescents (n = 111) participating in the I.Family Study. Subjects were divided into two groups based upon self‐reported sleep duration, according to the recommended amount of sleep for paediatric populations. Sleep needs for children <13 years were at least 9 h per day, and for children >13 were at least 8 h per day. There were group differences (short sleepers versus normal sleepers) in circulating levels of miR‐26b‐3p (mean (95% CI) = 2.0 (1.3–2.7) versus 2.3 (1.9–2.7), P = 0.05) and miR‐485‐5p (mean (95% CI) = 0.6 (0.3–0.9) versus 0.9 (0.7 – 1.0), P < 0.001), adjusting for country of origin, age, sex, pubertal status, screen time and highest educational level of parents. Our findings show for the first time that sleep duration reflects the profile of specific circulating microRNAs in school‐aged children and adolescents. It is conceivable that epigenetic modifications, mainly related to circadian rhythm control, may be modulated or interfere with sleep duration.
Tabata‐style functional exercise increases resting and postprandial fat oxidation but does not reduce triglyceride concentrations
Authors: Pearson Regis C. , Olenick Alyssa A. , Green Edward S. , Jenkins Nathan T. 
Published: January 2020

New Findings
What is the central question of this study?
What are the metabolic impacts of high intensity functional Tabata exercise?

What is the main finding and its importance?
Tabata exercise with high intensity functional movements causes increases in fasted and postprandial fat oxidation the day after exercise without altering postprandial triglyceride concentrations. These results support the usage of a Tabata‐style high intensity functional exercise to improve postprandial fat oxidation.

AbstractWe evaluated the effect of a high fat meal with and without prior high intensity functional exercise executed in a Tabata‐style interval pattern on resting and postprandial substrate oxidation, as well as postprandial blood glucose and triglyceride concentrations. Eleven healthy males completed two trials (Tabata exercise (TE) and non‐exercise control (CON)) in random order separated by 7 days. A two‐day protocol was used in which TE or CON was performed on the first day and a high fat meal was administered ∼13 h later the following morning. Power output from the TE session was quantified using a kinematic approach by calculating external work performed per unit time for each of the four exercises (rowing, dumbbell thrusters, kettlebell swings and burpees). For the meal challenge, respiratory gases and blood samples were taken fasted and at 1, 2 and 3 h postprandial. Fat oxidation was significantly higher after TE compared to CON at all time points (P < 0.05). Carbohydrate oxidation was significantly lower after TE compared to CON at 1 h postprandial (P < 0.05). There were no significant effects of TE on fasting or postprandial glucose or triglyceride concentrations. Functional exercises performed in a high intensity TE pattern enhance fasting and postprandial fat oxidation on the following day with minimal influence on blood triglycerides or glucose levels.
Study protocol for a phase II, multicentre, prospective, non-randomised clinical trial to assess the safety and efficacy of infusing allogeneic activated and expanded natural killer cells as consolidation therapy for paediatric acute myeloblastic leukaemia
Authors: Muñoz Builes Mario , Vela Cuenca María , Fuster Soler Jose L , Astigarraga Itziar , Pascual Martínez Antonia , Vagace Valero Jose M , Tong Hoi Y , Valentín Quiroga Jaime , Fernández Casanova Lucía , Escudero López Adela , Sisinni Luisa , Blanquer Miguel , Mirones Aguilar Isabel , González Martínez Berta , Borobia Alberto M , Pérez-Martínez Antonio 
Published: January 2020

IntroductionAcute myeloblastic leukaemia (AML) constitutes the second most common haematological malignancy in the paediatric population. Current treatment regimens are based on the administration of polychemotherapy, combining high doses of cytarabine with anthracyclines and topoisomerase inhibitors. Allogeneic haematopoietic stem cell transplantation (HSCT) is an option for high-risk patients with AML (and for intermediate-risk patients if a sibling donor is available). With this strategy, AML survival has increased substantially; however, it has remained stagnant at approximately 60%, with relapse being the principal culprit. The predominant role of the immune system and natural killer (NK) cells in controlling paediatric AML has gained importance within the context of HSCT. In this protocol, we propose incorporating this cell therapy as an adjuvant treatment through the infusion of activated and expanded haploidentical NK (NKAE) cells in paediatric patients with AML who are in cytological remission after completing consolidation therapy, and with no indication for HSCT.Methods and analysisPatients up to 30 years of age, diagnosed with AML, in their first cytological remission, who have completed both the induction and the consolidation phases of chemotherapy and do not meet the criteria for allogeneic HSCT are eligible. The patients will receive two doses of NKAE cells once a week, using a GMP K562-mbIL15-41BBL stimulus from a haploidentical donor and interleukin 2 subcutaneously. The patients will then be followed up for 36 months to assess the primary endpoint, which is the probability of relapse after NK cell infusion.Ethics and disseminationThis clinical trial was approved by the Clinical Research Ethics Committee of La Paz University Hospital and The Spanish Agency of Medicines and Medical Devices. Findings will be disseminated through peer-reviewed publications, conference presentations and community reporting.Trial registration numberEudraCT code: 2015-001901-15, ClinicalTrials.gov Identifier:NCT02763475.
Copper intrauterine device use and HIV acquisition in women: a systematic review
Authors: Hannaford Philip C , Ti Angeline , Chipato Tsungai , Curtis Kathryn M 
Published: January 2020

ObjectivesTo review systematically copper intrauterine device (Cu-IUD) use and HIV acquisition in women.MethodsWe searched Pubmed, Embase and the Cochrane Library between database inception and 26 June 2019 for longitudinal studies comparing incident HIV infection among women using an unspecified IUD or Cu-IUD compared with non-hormonal or no contraceptive users, or hormonal contraceptive users. We extracted information from included studies, assessed study quality, and summarised study findings.ResultsFrom 2494 publications identified, seven met our inclusion criteria. One randomised controlled trial (RCT), judged “informative with few limitations”, found no statistically significant differences in HIV risk between users of the Cu-IUD and either intramuscular depot medroxyprogesterone acetate (DMPA-IM) or levonorgestrel implant. One observational study, deemed “informative but with important limitations”, found no statistically significant difference in HIV incidence among IUD users compared with women who had tubal ligation or who were not using any contraception. Another “informative but with important limitations” observational study found no difference in HIV incidence between Cu-IUD users and DMPA or norethisterone enanthate injectable, or implant users. An RCT considered “unlikely to inform the primary question” also found no difference in HIV risk between Cu-IUD and progestogen-only injectable users. Findings from the other three “unlikely to inform the primary question” cohort studies were consistent with the more robust studies suggesting no increased risk of HIV acquisition among Cu-IUD users.ConclusionThe collective evidence, including that from a large high-quality RCT, does not indicate an increased risk of HIV acquisition among users of Cu-IUDs.
Inadvertent tissue adhesive tarsorrhaphy of the eyelid: a review and exploratory trial of removal methods of Histoacryl
Authors: Liu Zhenghong , Lim Yi Ting , Leong Kwok Fai, Mark 
Published: January 2020

ObjectiveThe use of tissue adhesives has become a popular option for closure of wounds in the ED. There have been a growing number of reports of inadvertent tissue adhesive injuries including closure of the eyelids. We aim to identify and compare various removal methods of tissue adhesives described in the literature in an exploratory trial.MethodsA review was first conducted to establish all published methods for the removal of medical-grade tissue adhesives as well as commercial cyanoacrylates. This search was conducted on PubMed, Google Scholar and Google. All articles that reported attempts at removal of cyanoacrylate glues were included. These methods were then tested on a porcine model in an exploratory trial. Incisions were made on pigskin and closed with Histoacryl, a tissue adhesive. Three removal methods were tested—gentle rubbing with test compound after 45 or 90 s, as well as soaking in test compound. Removal methods that were successful underwent repeat testing.ResultsA total of 37 sources were reviewed with 13 different removal methods suggested. Based on the information, we tested 24 different compounds. Soaking of Histoacryl-closed wounds in Polydexa ear/eye drops displayed consistent success in achieving complete separation of incision edges after 2 hours. Several other soapy substances and antibiotic ointments showed potential but were not as consistent.ConclusionIn conclusion, in our trial of removal methods of Histoacryl, soaking in Polydexa antibiotic drops consistently facilitated removal after 2 hours. This approach can be attempted after inadvertent Histoacryl injury.
Association between hypertension and retinal vascular features in ultra-widefield fundus imaging
Authors: Robertson Gavin , Fleming Alan , Williams Michelle Claire , Trucco Emanuele , Quinn Nicola , Hogg Ruth , McKay Gareth J , Kee Frank , Young Ian , Pellegrini Enrico , Newby David E , van Beek Edwin J R , Peto Tunde , Dhillon Baljean , van Hemert Jano , MacGillivray Thomas J 
Published: January 2020

ObjectiveChanges to the retinal vasculature are known to be associated with hypertension independently of traditional risk factors. We investigated whether measurements of retinal vascular calibre from ultra-widefield fundus imaging were associated with hypertensive status.MethodsWe retrospectively collected and semiautomatically measured ultra-widefield retinal fundus images from a subset of participants enrolled in an ongoing population study of ageing, categorised as normotensive or hypertensive according to thresholds on systolic/diastolic blood pressure (140/90 mm Hg) measured in a clinical setting. Vascular calibre in the peripheral retina was measured to calculate the nasal–annular arteriole:venule ratio (NA-AVR), a novel combined parameter.ResultsLeft and right eyes were analysed from 440 participants (aged 50–59 years, mean age of 54.6±2.9 years, 247, 56.1% women), including 151 (34.3%) categorised as hypertensive. Arterioles were thinner and the NA-AVR was smaller in people with hypertension. The area under the receiver operating characteristic curve of NA-AVR for hypertensive status was 0.73 (95% CI 0.68 to 0.78) using measurements from left eyes, while for right eyes, it was 0.64 (95% CI 0.59 to 0.70), representing evidence of a statistically significant difference between the eyes (p=0.020).ConclusionsSemiautomated measurements of NA-AVR in ultra-widefield fundus imaging were associated with hypertension. With further development, this may help screen people attending routine eye health check-ups for high blood pressure. These individuals may then follow a care pathway for suspected hypertension. Our results showed differences between left and right eyes, highlighting the importance of investigating both eyes of a patient.
Meningococcal vaccine uptake among men who have sex with men in response to an invasive meningococcal C disease outbreak in Melbourne, Australia
Authors: Martín-Sánchez Mario , Fairley Christopher K , Bradshaw Catriona S , Chen Marcus Y , Chow Eric P F 
Published: January 2020

ObjectiveIn 2017, there was an outbreak of invasive meningococcal disease (IMD) serogroup C among men who have sex with men (MSM) in Victoria, Australia. A government-funded free meningococcal (MenACWY) vaccination programme targeting all MSM living in Victoria was launched between December 2017 and December 2018. The aim of this study was to examine the vaccine uptake among MSM attending a sexual health clinic in Melbourne.MethodsThis was a retrospective clinical audit of MSM attending the Melbourne Sexual Health Centre (MSHC) during the vaccination programme. We calculated the proportion of MSM who received the meningococcal vaccine on their first visit and at any time during the programme. We performed univariable and multivariable logistic regression to identify the factors associated with the vaccine uptake on the first visit.ResultsOf the 10 370 MSM who attended MSHC, 55.5% received the vaccine on their first visit and 67.4% at any time during the programme. MSM had higher odds of receiving the vaccine on the first visit if they were aged 16–25 years (adjusted OR (aOR) 1.21; 95% CI 1.08 to 1.35) or 26–35 years (aOR 1.17; 95% CI 1.07 to 1.29) in comparison with MSM older than 35 years; were HIV-negative and not on pre-exposure prophylaxis (aOR 1.80; 95% CI 1.56 to 2.09); had more than four male partners in the last 12 months (aOR 1.16; 95% CI 1.06 to 1.27); had male partners only (aOR 2.24; 95% CI 1.96 to 2.55); or were born overseas (aOR 1.11; 95% CI 1.03 to 1.21).ConclusionsTwo-thirds of the MSM attending a sexual health clinic received at least one dose of meningococcal vaccine. The vaccination programme coincided temporally with a dramatic reduction in the incidence of IMD. Vaccination should be further promoted among MSM and men who have sex with both men and women.
High-resolution radioactive beam study of the $$^{26}\hbox {Al}(d,p$$) reaction and measurements of single-particle spectroscopic factors
Authors: Lotay G. , Woods P. J. , Moukaddam M. , Aliotta M. , Christian G. , Davids B. , Davinson T. , Doherty D. T. , Howell D. , Margerin V. , Ruiz C. 
Published: January 2020

AbstractWe present a detailed comparison of shell model calculations with inverse kinematic transfer reaction data, obtained using a radioactive beam. Experimentally extracted spectroscopic factors from the $$^{26}\hbox {Al}(d,p)^{27}\hbox {Al}$$26Al(d,p)27Al reaction for both even and odd parity states are found to be exceptionally well reproduced by the shell model and a high level of consistency is observed between bound isobaric analog states in $$^{27}\hbox {Al}$$27Al and $$^{27}\hbox {Si}$$27Si, populated via (d, p) and (d, n) transfer, respectively. Furthermore, an evaluation of key resonances in the astrophysical $$^{26}\hbox {Al}(p,\gamma )^{27}\hbox {Si}$$26Al(p,γ)27Si reaction indicates that shell model calculations provide relatively accurate predictions for the existence of strong resonances and mirror nucleus comparisons appear to hold exceptionally well for proton-unbound levels. Consequently, we expect that the utilization of both techniques will likely be a very effective tool in the investigation of stellar processes outside the current reach of experiment.