bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reprogramming of mouse embryonic fibroblasts into induced oligodendrocyte progenitor cells via nanovector-mediated indirect lineage conversion
Authors: Xiao Xiong , Ling Wen-Hui , Qiu Xiao-Yan , Wang Ming-Yu , Xiong Chun-Xia , Xie Deng-Feng , Chu Xin-Yue , Li Yun-Xin , Huang Yun , Li Tong , Li Yue-Min 
Published: June 2020

Abstract
Background: Axon-wrapping myelin sheath is vital to nerve conduction, loss or dysfunction of it will result in some neurological diseases. Since mature oligodendrocytes (OLs) lack proliferation ability, and oligodendrocyte progenitor cells (OPCs) maintain slow proliferation or quiescence state, transplantation of exogenous OPCs becomes an alternative strategy for curing these diseases. Methods: OPCs were isolated from brain tissue and generated from NIH/3T3 cells via indirect lineage conversion here. Results: 0.25 % chicken serum was conducive to trypsin-mediated digestion of cerebral cortex, the modified shaking method could improve the purification efficiency of OPCs. Poly-MAG was a safer and higher efficient nanovector for delivering plasmid DNA into cells. Forced expressions of Olig 2, Nkx 6.2, and Sox 10 could attenuate the expressions of fibroblast-related genes (Col5a1 and Col1a1), up-regulate OPC-associated genes (PDGFRα, S100β, NG2, and Olig 2), and finally successfully reprogram NIH/3T3 cells into induced oligodendrocyte progenitor cells (iOPCs). 1 μM haloperidol (HAL) contributed to promoting the proliferation of iOPCs. These iOPCs had the potential to be differentiated into OLs and type Ⅱ astrocytes. Conclusions: The high-efficiency and low-cytotoxicity strategies for isolating mouse primary OPCs and generating iOPCs were established, which provided novel cell resources for disease modeling, drug screening, cell therapy, and so on.
Looking into mechanisms of change: Evaluation of a multifaceted implementation strategy to change pain management behavior of care workers
Authors: Brunkert Thekla , Simon Michael , Zúñiga Franziska 
Published: June 2020

Abstract
BackgroundUnderutilization of evidence-based pain management in nursing homes is common. Evidence towards effective approaches to improve adoption of evidence-based practices in nursing homes is limited. To get a better understanding of the impact of our multi-faceted implementation strategies, care worker training workshops and the introduction of trained pain champions, this study explored the underlying mechanisms of the implementation strategies using behavioral theory.MethodsWe conducted a mixed-methods evaluation alongside an implementation- effectiveness study in four Swiss nursing homes. Based on an a priori contextual analysis in the participating homes implementation strategies were developed. Furthermore, we developed a conceptual framework describing hypotheses concerning determinants of implementation and mechanisms of change underlying our implementation strategies.Care workers’ questionnaire surveys were conducted at baseline (n=136), after three (n= 99) and six months (n=83) to assess self-efficacy in pain management and self-reported guideline adoption. We computed linear mixed-effect models to assess changes over time in self-efficacy and logistic regressions to assess associations between self-efficacy and guideline adoption. Concurrently, we conducted focus groups with care workers (n=8) to explore their response to the implementation strategies and to gain a deeper understanding of the potential mechanisms. After transcription, interview data was analyzed using content analysis.ResultsOverall, there was a significant increase in self-efficacy after three and six months (p<0.001). Self-reported adoption of guideline components ranged between 44% and 73% depending on the component. We found significant associations between self-efficacy and adoption of two guideline components, i.e. performing a comprehensive pain assessment and using an observational pain assessment tool in cognitively impaired residents.Qualitative findings showed that the training workshops and pain champions were received positively by care workers. Focus group participants reported to be more attentive to residents` pain experience and to assess and document pain more frequently and with more detail than before.ConclusionsOur findings highlight the importance of continuous commitment of an implementation facilitator, e.g., a pain champion, within an organization. Regarding persistent implementation challenges, a theory-based conceptual model contributes to the overall understanding.Trial registrationClinicalTrials.gov (NCT03471390)
Long-Term Survival After Carpentier-Edwards Perimount Aortic Valve Replacement in Denmark: A Multi-Centre Observational Study
Authors: Krasniqi Lytfi , Kronby Mads Philip , Riber Lars Peter Schødt 
Published: June 2020

Abstract
Background: This study describes the long-term survival, risk of reoperation and clinical outcomes for patients undergoing solitary surgical aortic valve replacement (SAVR) with a Carpentier-Edwards Perimount (CE-P) bioprosthetic in Denmark. The renewed interest in SAVR is based on the questioning regarding the long-term survival since new aortic replacement technique such as transcatheter aortic-valve replacement (TAVR) probably have shorter durability, why assessment of long-term survival could be a key issue for patients.Methods: From November 1999 to November 2015 a cohort of a total of 1604 patients aged 20-91 undergoing solitary SAVR with CE-P in Western Denmark was obtained November 2018 from the Western Danish Heart Registry (WDHR). The primary endpoint was long-term survival from all-cause mortality. Secondary endpoints were survival free from major adverse cardiovascular and cerebral events (MACCE), risk of reoperation, cause of late death, patient-prothesis mismatch, risk of AMI, stroke, pacemaker or ICD implantation and postoperative atrial fibrillation (POAF). Time-to event analysis was performed with Kaplan-Meier curve, cumulative incidence function was performed with Nelson-Aalen cumulative hazard estimates. Cox regression was applied to detect risk factors for death and reoperation.Results: In-hospital mortality was 2.7% and 30-day mortality at 3.4%. The 5-, 10- and 15-year survival from all-cause mortality was 77%, 52% and 24%, respectively. Survival without MACCE was 80% after 10 years. Significant risk factors of mortality were small valves, smoking and EuroSCORE II ≥4%. The risk of reoperation was <5% after 7.5 years and significant risk factors were valve prosthesis-patient mismatch and EuroSCORE II ≥4%.Conclusions: Patients operated with for aortic valve replacement with a Carpentier-Edwards Perimount valve shows a very satisfying long-term survival. Future research should aim to investigate biological valves long-term durability for comparison of different SAVR to different TAVR in long perspective.
Mutation of OsGUN4 Uncoupled the Sugar-dependent Signals to Regulate Starch Biosynthesis in Rice
Authors: Li Ruiqing , Jiang Meng , Li Min , Zhang Huali 
Published: June 2020

Abstract
Background

Starch biosynthesis requires plastid-to-nucleus signals to ordinate the flow of carbon, which is partly mediated by tetrapyrrole intermediates. We previously revealed that mutation of the Genomes Uncoupled 4 (OsGUN4) would affect tetrapyrrole intermediates, but the underlying mechanisms for regulatory roles of OsGUN4 on starch biosynthesis remains largely unknown.
Results

In this study, we revealed that the OsGUN4 mutation not only retarded the carbon flow from sucrose to starch but also disabled the sensitive response to exogenous feeding sucrose. Moreover, extra addition of norflurazon (NF) would aggravate insensitivity to the sucrose-dependent induction in gun4epi, especially exhibiting collapse declines of the starch biosynthetic enzymes’ activities. However, genes encoding starch synthetic enzymes performed discordance with the activities of starch biosynthesis associated enzymes upon on the scarce expression of OsGUN4 in gun4epi. These results indicated that OsGUN4 played regulatory roles on biosynthetic genes and enzyme activity in starch biosynthesis. Furthermore, we also concluded that 1O2 derived from GUN4/protoporphyrin IX (proto) might be responsible for the sugar-dependent signals to regulate starch biosynthesis, due to positive correlations between the singlet oxygen (1O2) and many starch biosynthetic genes that were subjected to the control of three reported transcriptional factors (TFs) during starch biosynthesis. Eventually, the OsGUN4 mutation would also relieve the repression of glucose on Snf1-related protein kinases (SnRK1) but seem to negatively mediate the functioning of ADP-Glc pyrophosphorylase (AGPase).
Conclusion

In summary, we demonstrated that OsGUN4 serve as a broker to activate 1O2-mediated signals in the sugar signaling cascade, possibly functioning upstream through TFs, and that OsGUN4 played roles in the SnRK1A-mediated signals, partly through the accumulations of sugars, e.g. glucose or sucrose.
Identification of Prognostic Biomarkers and Immunotherapeutic Targets with CXC Chemokines in Glioblastoma Multiforme Using Integrated Bioinformatic Analysis
Authors: Liu Hailing , Zhu Jinguang , Wang Guangwen 
Published: June 2020

Abstract
Background Glioblastoma multiforme (GBM) is the most malignant central nervous system tumour bearing a dismal prognosis. The study aimed to explore the potential biomarkers and therapeutic targets with CXC chemokines in GBM by integrated bioinformatics analysis.Methods Differentially expressed CXC Chemokines were identified in GBM using GEPIA and UALCAN databases,and Kaplan–Meier analyses were performed by GEPIA subsequently. Protein -protein interaction (PPI) network was established in STRING database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis were utilized to analyze differentially expressed CXC Chemokines and their similar genes gained from GEPIA. Then, we conducted transcription factors, kinase targets, and immune cells infiltration using TRRUST, LinkedOmics, and TIMER, respectively.Results The mRNA expression levels of CXCL3/5/6/9/10/11/12/13/16 in GMB were significantly elevated compared to normal tissues. GBM patients with higher transcriptional levels of CXCL5/6 were significantly associated with worse disease-free survival, while higher transcriptional levels of CXCL3/5/8 were significantly related to worse overall survival. The functions of CXC chemokines were enriched in Chemokine signaling pathway, Cytokine-cytokine receptor interaction, IL-17 signaling pathway, et.al. RELA and NFKB1were key transcription factors of CXC chemokines. The kinase targets of CXC chemokine contained CDK1, CDK2, PRKCD, MAPK14, ATM, LCK, MTOR, and GRK3, which are involved in oncogenesis, migration, and survival. Moreover, we revealed significant correlations between the expression of CXC chemokines and the infiltration immune cells, especially for dendritic cells.Conclusion The significant CXC chemokines and related pathways may provide a novel possibility for prognostic biomarkers and immunotherapeutic treatment in GMB.Short title: CXC Chemokines with prognosis in GBM
Establishment of Prediction Models for venous thromboembolism in non-oncological urological inpatients--a single center experience
Authors: Li Kaixuan , Li Haozhen , Zhu Quan , Wu Ziqiang , Wang Zhao , Tang Zhengyan 
Published: June 2020

Abstract
Background

To establish prediction models for venous thromboembolism (VTE) in non-oncological urological inpatients.
Methods

A retrospective analysis of 1453 inpatients was carried out and the risk factors for VTE had been clarified our previous studies.
Results

Risk factors included the following 5 factors: presence of previous VTE (X1), presence of anticoagulants or anti-platelet agents treatment before admission (X2), D-dimer value (≥ 0.89 µg/ml, X3), presence of lower extremity swelling (X4), presence of chest symptoms (X5). The logistic regression model is Logit (P) = − 5.970 + 2.882 * X1 + 2.588 * X2 + 3.141 * X3 + 1.794 * X4 + 3.553 * X5. When widened the p value to not exceeding 0.1 in multivariate logistic regression model, two addition risk factors were enrolled: Caprini score (≥ 5, X6), presence of complications (X7). The prediction model turns into Logit (P) = − 6.433 + 2.696 * X1 + 2.507 * X2 + 2.817 * X3 + 1.597 * X4 + 3.524 * X5 + 0.886 * X6 + 0.963 * X7. Internal verification results suggest both two models have a good predictive ability, but the prediction accuracy turns to be both only 43.0% when taking the additional 291 inpatients’ data in the two models.
Conclusion

We built two similar novel prediction models to predict VTE in non-oncological urological inpatients.
Trial registration:
This trial was retrospectively registered at http://www.chictr.org.cn/index.aspx under the public title“The incidence, risk factors and establishment of prediction model for VTE n urological inpatients” with a code ChiCTR1900027180 on November 3, 2019. (Specific URL to the registration web page: http://www.chictr.org.cn/showproj.aspx?proj=44677).
High SMARCA5 Expression is Associated With Poor Prognosis in Patients With Non-Small Cell Lung Cancer
Authors: Yang Zhi-Qiang , wen yuanyuan , Li Hai-Feng , Li Lue , He Hui 
Published: June 2020

Abstract
Background: SMARCA5 has been proved to play the role of oncogene in various types of tumors. The purpose of our study was to study the relationship between the expression of SMARCA5 and the prognosis of patients with Non-small cell lung cancer(NSCLC), and to investigate the effect of silencing the expression of SMARCA5 on the biological behavior of NSCLC. Methods：Immunohistochemistry method was adopted to detect to the expressions of SMARCA5 proteins in NSCLC and normal lung tissues. The correlation between the expression of SMARCA5 and clinicopathological factors and survival prognosis of patients with NSCLC were analyzed by statistical methods. Western blot and RT-PCR were used to detect the expression of SMARCA5 in normal human bronchial epithelial cell line (HBE) and human lung cancer cell lines (A549, Lu165, SK-MES-1 and NCI-H520). SMARCA5 siRNA was employed to silence the the expression of SMARCA5. MTT and Transwell methods were used to detect the proliferation and invasion of lung cancer cells.Results: SMARCA5 protein were highly expressed in 78 (59.5%) NSCLC tissues. SMARCA5 mRNA (P<0.05) and protein (P<0.05) were significantly higher in NSCLC tissues than in the adjacent normal tissues. SMARCA5 expression was significantly associated with poorly differentiation (P<0.001), advanced TNM stage (P<0.001), and pleural invasion (P=0.032). Meanwhile, High expression of SMARCA5 was associated with poor prognosis of NSCLC patients. Knockdown of SMARCA5 expression inhibited cell growth and invasion in NSCLC. Conclusion: High expression of SMARCA5 is associated with poor prognosis in patients with NSCLC, and SMARCA5 can increase activity of proliferation and invasion in NSCLC cells. SMARCA5 may play a decisive role in tumorigenesis of NSCLC.
A Pilot Case-Control Study Using a One Health Approach to Evaluate Behavioral, Environmental, and Occupational Risk Factors for Chronic Kidney Disease of Unknown Etiology in Sri Lanka
Authors: Pry Jake , Jackson Wendi , Rupasinghe Ruwini , Lishanthe Guneratne , Badurdeen Zied , Abeysekara Tilak , Chandrajith Rohana , Smith Woutrina , Wickramasinghe Saumya 
Published: June 2020

Abstract
Background: Chronic kidney disease of unknown etiology (CKDu) was first recognized in Sri Lanka in the early 1990s, and since then it has reached epidemic levels in the North Central Province of the country. The prevalence of CKDu is reportedly highest among communities that engage in chena and paddy farming, which is most often practiced in the dry zone including the North Central and East Central Provinces of Sri Lanka. Previous studies have suggested varied hypotheses for the etiology of CKDu; however, there is not yet a consensus on the primary risk factors, possibly due to disparate study designs, sample populations, and methodologies.Methods: The goal of this pilot case-control study was to evaluate the relationships between key demographic, cultural, and occupational variables as risk factors for CKDu, with a primary interest in pesticide exposure both occupationally and through its potential use as an ingredient in brewed kasippu alcohol. An extensive one health focused survey was developed with in cooperation with the Centre for Research, Education, and Training on Kidney Diseases of Sri Lanka. Results: A total of 56 CKDu cases and 54 control individuals were surveyed using a proctored, self-reported questionnaire. Occupational pesticide exposure and alcohol consumption were not found to be significant risk factors for CKDu. However, a statistically significant association with CKDu was observed with chewing betel (adjusted odds ratio [aOR]: 6.11, 95% confidence interval [CI]: 1.93, 19.35), age (aOR: 1.07, 95% CI: 1.02, 1.13), owning a pet dog (aOR: 3.74, 95% CI: 1.38, 10.11), water treatment (aOR: 3.68, 95% CI: 1.09, 12.43) and pests in the house (aOR: 5.81, 95% CI: 1.56, 21.60).Conclusions: The findings of this study suggest future research should focus on practices associated with chewing betel, potential animal interactions including pests in the home and pets, and risk factors associated with water.
The Dosimetric and Clinical Comparison between Helical Tomotherapy and Fixed-Field Intensity-Modulated Radiotherapy in Radical Irradiation for Cervical Cancer
Authors: Guo Mingfang , Ran Xueqi , Zou Dongling , Zhang Na , Liu Xianfeng 
Published: June 2020

Abstract
Backgroud: To evaluate the dosimetric parameters, clinical complications, and efficacy of helical tomotherapy(HT) and fixed-field intensity-modulated radiotherapy (f-IMRT) in radical radiotherapy for cervical cancer.Methods: Between November 2016 and December 2018, 77 cervical cancer patients in radical irradiation were enrolled, 38 patients undergoing treatment with HT and 39 with f-IMRT. The dosimetry parameters, clinical complications, and efficacy were compared. Results: The HT plans had superior homogeneity index(HI) , conformity index(CI) (P=0.000),and resulted in a reduction in the dosimetry parameters of organs at risk(OARs) (P<0.05) except the V10 of small intestine (P=0.682).The incidence of myelosuppression showed no significant differences(P=0.265).The patients with HT had no radioactive cystitis, grade 2 or above rectal complication and acute bladder complication. The complete remission(CR) rates ,effective rates(CR+PR) and local control rates of two years were 81.58%,100% and 97.37%.Conclusion: The HT plans showed advantages in dosimetry, and provided more superior clinical outcomes.
Pemilihan Cemilan Khas Sampit Terlaris Pada Kedai 24 Dengan Metode AHP (Analytic Hierarchy Process)
Authors: Yanti Yuli , Safitri Diah Atika , Alamsyah Rendy Alamsyah 
Published: June 2020

<p>Penentuan cemilan khas Sampit terlaris pada Kedai 24 adalah salah satu hal yang penting, sebab dengan menentukan produk apa yang terlaris, kedai 24 dapat memprediksi produk apa yang harus diproduksi lebih banyak. Salah satu cara yang dapat dilakukan untuk memilih produk adalah dengan cara menerapkan sistem penunjang keputusan. Dengan menerapkan sisitem penunjang keputusan pemilik kedai dapat memutuskan dengan hasil yang tepat dalam memilih produk terlaris. Ada beberapa kriteria pilihan yang diterapkan dan ada beberapa alternatif pilihan yang dapat dipilih dalam memilih produk yang terlaris. Analisis data menerapkan metode Analytical Hierarchical Process (AHP), yang memungkinkan perhitungan dengan berbagai kriteria yang ditentukan pemilik kedai. Hasil perhitungan yang telah dilakukan didapatkan rata-rata kriteria rasa sebesar 0,633346, rata-rata kriteria harga sebesar 0,260498 dan rata-rata kriteria kemasan sebesar 0,106156. Setelah dilakukan perhitungan dengan masing-masing alternatif yang ada, didapatkan hasil tertinggi yaitu produk jenis Abon sebesar 0,48.</p>
Identifikasi Potensi Keberhasilan Studi Menggunakan Naïve Bayes Classifier
Authors: Yuniarti Wenty Dwi , Faiz Achmad Nur , Setiawan Bagus 
Published: June 2020

<p>Penelitian ini bertujuan melakukan prediksi keberhasilan studi dengan metode klasifikasi <em>naïve bayes classifier</em>. Varibel input yang diperkirakan mempengaruhi keberhasilan studi adalah Jalur Masuk (1), Asal Kota (2), Asal Provinsi (3), Penghasilan Orang Tua (4), Pekerjaan Orang Tua (5), Indeks Prestasi Kumulatif (6) serta Riwayat Status Mahasiswa (7). Pengetahuan potensi keberhasilan studi diperoleh dari variasi empat variabel target (<em>class</em>) yaitu IPK Tahun Pertama dan Kedua, Status Mahasiswa Terkini, IPK Mata Kuliah (Makul) Non-Keprodian serta IPK Makul Keprodian. Proses diawali <em>data</em><em> preprocessing</em> dan diperoleh 5.934 data bersih. Data dibagi 80% <em>training</em>, 20% <em>testing</em>, dengan <em>Correctly Classified Instances</em> 97,53%. Penggalian pengetahuan dengan <em>naïve bayes classifier</em> memperoleh akurasi 99,41% untuk prediksi variabel input 1,2,3,4,5,6,7 dengan target keberhasilan IPK Tahun pertama dan kedua, 96.96 %, untuk target Status Mahasiswa Terkini, 95.87% untuk target IPK Makul Keprodian, dan 97.89 % untuk target IPK Makul Non-Keprodian. Penggunaan metode <em>naïve bayes classifier</em> dalam klasifikasi potensi keberhasilan studi ini memberikan akurasi 95.8% sampai dengan 99.41% untuk 4 target berbeda. Bagi perguruan tinggi, perlu penguatan pada proses perekrutan mahasiswa, serta perlu diperhatikan bahwa faktor ekonomi orang tua memberikan andil bagi kelangsungan proses studi.</p>
Implementasi Dan Analisis Server Bigbluebutton Dalam Pemanfaatan Meeting Online Pada Masa Pandemic
Authors: Kusuma Alan Budi , Yusuf Amir , Hidayat Alfian 
Published: June 2020

The need for video converence is something that cannot be ignored. Amid the Covid-19 pandemic outbreak all sectors must utilize this technology. Such is the case with Semarang Islamic State University Walisongo. Where UIN Walisongo requires all students, lecturers and education personnel to remain at home in an effort to comply with government regulations as an effort to prevent this outbreak. Therefore, to find a solution for communication to stay connected, a system for online meetings was built that could be used by students and lecturers as a medium of online learning, and the education staff and leaders to be able to always be connected in the midst of the covid outbreak.