bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuronal maturation reduces the type I IFN response to orthobunyavirus infection and leads to increased apoptosis of human neurons
Authors: Winkler Clayton W. , Woods Tyson A. , Groveman Bradley R. , Carmody Aaron B. , Speranza Emily E. , Martens Craig A. , Best Sonja M. , Haigh Cathryn L. , Peterson Karin E. 
Published: November 2019

AbstractBackgroundLa Crosse virus (LACV) is the leading cause of pediatric arboviral encephalitis in the USA. LACV encephalitis can result in learning and memory deficits, which may be due to infection and apoptosis of neurons in the brain. Despite neurons being the primary cell infected in the brain by LACV, little is known about neuronal responses to infection.MethodsHuman cerebral organoids (COs), which contain a spectrum of developing neurons, were used to examine neuronal responses to LACV. Plaque assay and quantitative reverse transcription (qRT) PCR were used to determine the susceptibility of COs to LACV infection. Immunohistochemistry, flow cytometry, and single-cell transcriptomics were used to determine specific neuronal subpopulation responses to the virus.ResultsOverall, LACV readily infected COs causing reduced cell viability and increased apoptosis. However, it was determined that neurons at different stages of development had distinct responses to LACV. Both neural progenitors and committed neurons were infected with LACV, however, committed neurons underwent apoptosis at a higher rate. Transcriptomic analysis showed that committed neurons expressed fewer interferon (IFN)-stimulated genes (ISGs) and genes involved IFN signaling in response to infection compared to neural progenitors. Furthermore, induction of interferon signaling in LACV-infected COs by application of recombinant IFN enhanced cell viability.ConclusionsThese findings indicate that neuronal maturation increases the susceptibility of neurons to LACV-induced apoptosis. This susceptibility is likely due, at least in part, to mature neurons being less responsive to virus-induced IFN as evidenced by their poor ISG response to LACV. Furthermore, exogenous administration of recombinant IFN to LACV COs rescued cellular viability suggesting that increased IFN signaling is overall protective in this complex neural tissue. Together these findings indicate that induction of IFN signaling in developing neurons is an important deciding factor in virus-induced cell death.
S100A10 silencing suppresses proliferation, migration and invasion of ovarian cancer cells and enhances sensitivity to carboplatin
Authors: Wang Lingzhi , Yan Wei , Li Xukun , Liu Zhihua , Tian Tian , Chen Tanxiu , Zou Liang , Cui Zhumei 
Published: November 2019

Abstract
Background
Ovarian cancer is the leading cause of gynecological cancer-related mortality. The novel oncogene S100A10 has been reported to be involved in cancer cell proliferation, invasion and metastasis. The role of S100A10 in ovarian cancer has not been well studied and the effect of S100A10 on chemotherapy remains unclear. The aims of the present study were to investigate the functional role of S100A10 in the progression and carboplatin sensitivity of ovarian cancer.

Methods
We examined the expression levels in tissues of S100A10 in 138 cases of ovarian cancer by IHC. To determine the functional roles of downregulated S100A10 in ovarian cancer, cell proliferation, colony formation, cell migration and invasion assays were performed. Chemoresistance was analyzed by apoptosis assay. A xenograft tumor model was established to confirm the role of S100A10 in carboplatin resistance in vivo. Using Western blot assays, we also explored the possible mechanisms of S100A10 in ovarian cancer.

Results
The results showed that increased expression of S100A10 was positively associated with carboplatin resistance (P < 0.001), tumor grade (P = 0.048) and a poorer prognosis (P = 0.0053). Functional analyses demonstrated that S100A10 suppression significantly suppressed ovarian cancer cell proliferation, colony formation, cell migration and invasion, remarkably increased carboplatin-induced apoptosis in SKOV3 and A2780 cells and inhibited tumor growth in vivo. Downregulation of S100A10 expression could inhibit cell proliferation and enhance ovarian cancer cell sensitivity to carboplatin, possibly involving the regulation of cleaved-Caspase3 and cleaved-PARP.

Conclusions
Together, the results of the present study reveal that S100A10 expression can be used as a predictive marker for the prognosis of ovarian cancer and chemosensitivity to carboplatin.
Potentiometric sensor for iron (III) quantitative determination: experimental and computational approaches
Authors: Badakhshan Somayeh , Ahmadzadeh Saeid , Mohseni-Bandpei Anoushiravan , Aghasi Majid , Basiri Amir 
Published: November 2019

AbstractThe current work deals with fabrication and validation of a new highly Fe3+ selective sensor based on benzo-18-crown-6 (b-18C6) using the potentiometric method. The proposed sensor revealed satisfactory performance for quantitative evaluation of Fe3+ trace amount in environmental samples. The ratio of membrane ingredients optimized and the membrane with the composition of 4:30:65.5:0.5 mg of b-18C6:PVC:o-NPOE:KTpClPB exhibited the desirable Nernstian slope of 19.51 ± 0.10 (mV per decade of activity) over the pH range from 2.5 to 5.7 with an acceptable dynamic concentration range of 1.0 × 10−6 M to 1.0 × 10−1 M and lower detection limit of 8.0 × 10−7 M. The proposed sensor demonstrated an appropriate reproducibility with a rapid response time of 12 s and the suitable lifetime of 10 weeks. To validate the accurate response of the proposed sensor, AAS technique applied for the determination of Fe3+ in real aqueous mediums such as drinking tap water and hospital wastewater sample after treatment by electrocoagulation process. Theoretical studies carried out using DFT/B3LYP computational level with 6-311G basis set to optimize the adsorption sites of Fe+3 cationic species by b-18C6. The obtained adsorption energy with large negative value confirmed the formation of a stable complex.
Eco-friendly consolidated process for co-production of xylooligosaccharides and fermentable sugars using self-providing xylonic acid as key pretreatment catalyst
Authors: Zhou Xin , Xu Yong 
Published: November 2019

Abstract
Background
Obtaining high-value products from lignocellulosic biomass is central for the realization of industrial biorefinery. Acid pretreatment has been reported to yield xylooligosaccharides (XOS) and improve enzymatic hydrolysis. Moreover, xylose, an inevitable byproduct, can be upgraded to xylonic acid (XA). The aim of this study was to valorize sugarcane bagasse (SB) by starting with XA pretreatment for XOS and glucose production within a multi-product biorefinery framework.

Results
SB was primarily subjected to XA pretreatment to maximize the XOS yield by the response surface method (RSM). A maximum XOS yield of 44.5% was achieved by acid pretreatment using 0.64 M XA for 42 min at 154 °C. Furthermore, XA pretreatment can efficiently improve enzymatic digestibility, and achieved a 90.8% cellulose conversion. In addition, xylose, the inevitable byproduct of the acid-hydrolysis of xylan, can be completely converted to XA via bio-oxidation of Gluconobacter oxydans (G. oxydans). Subsequently, XA and XOS can be simultaneously separated by electrodialysis.

Conclusions
XA pretreatment was explored and exhibited a promising ability to depolymerize xylan into XOS. Mass balance analysis showed that the maximum XOS and fermentable sugars yields reached 10.5 g and 30.9 g per 100 g raw SB, respectively. In summary, by concurrently producing XOS and fermentable sugars with high yields, SB was thus valorized as a promising feedstock of lignocellulosic biorefinery for value-added products.
Screening for thyroid dysfunction and treatment of screen-detected thyroid dysfunction in asymptomatic, community-dwelling adults: a systematic review
Authors: Reyes Domingo Francesca , Avey Marc T. , Doull Marion 
Published: November 2019

AbstractBackgroundThis systematic review was conducted to inform the Canadian Task Force on Preventive Health Care recommendations on screening for thyroid dysfunction (TD). The review sought to answer key questions on the benefits and harms of screening for TD, patients’ values and preferences for screening, and the benefits and harms of treating screen-detected TD.MethodsThis review followed Canadian Task Force on Preventive Health Care methods, which include the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach. The search strategy used for benefits and harms of screening and treatment was an update to the 2014 review by the US Preventive Services Task Force and searched MEDLINE and the Cochrane Library. MEDLINE, Embase, ProQuest Public Health, and SCOPUS were searched for patients’ values and preferences for screening. Outcomes of interest included all-cause mortality, deaths due to cardiovascular diseases, fatal and non-fatal cardiovascular events, atrial fibrillation, fractures, quality of life, cognitive function, and harms due to TD treatment. Two reviewers independently screened abstracts and full texts according to pre-determined inclusion criteria and assessed the risk of bias for each study included. Strength and quality of the evidence was assessed for each outcome. A narrative synthesis was conducted due to heterogeneity of the included studies.ResultsNo studies were found on screening for TD, treatment of subclinical hyperthyroidism, or patients’ values and preferences for screening for TD. Twenty-two studies (from 24 publications) on the treatment of TD in patients with screen-detected subclinical hypothyroidism were included. Results from the included randomized controlled trials suggested no benefit of treatment for subclinical hypothyroidism for the large majority of outcomes. We found very low-quality evidence (from two cohort studies) for a small reduction in all-cause mortality among adults < 65 or 40–70 years who were treated for TD compared to those who were not.ConclusionsThis review found moderate to very low-quality evidence on the benefits and harms of treatment for subclinical hypothyroidism, with most of the evidence showing no benefit of treatment.
Projected climate changes in four different regions in Colombia
Authors: Molina Oscar D. , Bernhofer Christian 
Published: November 2019

Abstract
Background
Considering the lack of research over this region the Statistical Downscaling Model (SDSM) was used as a tool for downscaling meteorological data statistically over four representative regions in the eastern side of Colombia. Data from the two Global Climate Models CanESM2 and IPSL-CM5A-MR, which are part of the CMIP5-project have been used to project future maximum and minimum temperature, precipitation and relative humidity for the periods 2021–2050 and 2071–2100. For both models, the Representative Concentration Pathways RCP2.6 and RCP8.5 were considered, representing two different possible future emission trajectories and radiative forcings. Predictor variables from the National Centre for Environmental Prediction (NCEP-DOE 2) reanalysis dataset, together with analyzed correlation coefficient (R) and root mean square error (RMSE) were used as performance indicators during the calibration and validation process.

Results
Results indicate that Maximum and minimum temperature is projected to increase for both Global Climate Models and both Representative Concentration Pathways; relative humidity shows a decreasing trend for all scenarios and all regions; and precipitation shows a slight decrease over three regions and an increase over the warmest region. As expected, the results of the simulation for the period 2071–2100 show a more drastic change when compared to the baseline period of observations.

Conclusions
The SDSM model proves to be efficient in the downscaling of maximum/minimum temperature as well as relative humidity over the studied regions; while showing a lower performance for precipitation, agreeing with the results for other statistical downscaling studies. The results of the projections offer good information for the evaluation of possible future-case scenarios and decision-making management.
Non-invasive identification of polymers in cultural heritage collections: evaluation, optimisation and application of portable FTIR (ATR and external reflectance) spectroscopy to three-dimensional polymer-based objects
Authors: Bell Julianne , Nel Petronella , Stuart Barbara 
Published: November 2019

Abstract
The conservation of polymer-based cultural heritage is a major concern for collecting institutions internationally. Collections include a range of different polymers, each with its own degradation processes and preservation needs, however, they are frequently unidentified in collection catalogues. Fourier transform infrared (FTIR) spectroscopy is a useful analytical tool for identifying polymers, which is vital for determining storage, exhibition, loan and treatment conditions. Attenuated Total Reflection (ATR), and External Reflection (ER) are proven effective FTIR sampling techniques for polymer identification and are beginning to appear in conservation labs. This paper evaluates and optimises the application of these two FTIR techniques to three-dimensional plastic objects in the museum context. Elements of the FTIR measurement process are investigated for 15 common polymers found in museum collections using both authentic reference sheets, and case study objects to model for surface characteristics. Including: use of the ATR and ER modules, the difference between clamping and manually holding objects in contact with the ATR crystal, use of the Kramers–Kronig Transformation, signal-to-noise ratios for increasing number of co-added scans, resultant time taken to collect each measurement, associated professional, health and safety considerations, and the use and availability of reference materials for polymer identify verification. Utilising this information, a flowchart for applying FTIR spectroscopy to three-dimensional historic plastic objects during museum collection surveys is proposed to guide the conservation profession.
Lost in translation: the valley of death across preclinical and clinical divide – identification of problems and overcoming obstacles
Authors: Seyhan Attila A. 
Published: November 2019

AbstractA rift that has opened up between basic research (bench) and clinical research and patients (bed) who need their new treatments, diagnostics and prevention, and this rift is widening and getting deeper. The crisis involving the “translation” of basic scientific findings in a laboratory setting into human applications and potential treatments or biomarkers for a disease is widely recognized both in academia and industry. Despite the attempts that have been made both in academic and industry settings to mitigate this problem, the high attrition rates of drug development and the problem with reproducibility and translatability of preclinical findings to human applications remain a fact and the return on the investment has been limited in terms of clinical impact.Here I provide an overview of the challenges facing the drug development, and translational discordance with specific focus on a number of “culprits” in translational research including poor hypothesis, irreproducible data, ambiguous preclinical models, statistical errors, the influence of organizational structures, lack of incentives in the academic setting, governmental funding mechanisms, the clinical relevance of basic research, insufficient transparency, and lack of data sharing in research. I further provide some suggestions and new strategies that include some new aspects on open innovation models, entrepreneurship, transparency, and decision making to overcome each of the many problems during the drug discovery and development process and to more dynamically adjust for innovation challenges with broader scientific feedback.
Pathogenicity of some commercial formulations of entomopathogenic fungi on the tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)
Authors: Ndereyimana Assinapol , Nyalala Samuel , Murerwa Patrick , Gaidashova Svetlana 
Published: November 2019

AbstractThe tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a major threat to tomato production in Rwanda. Laboratory bioassays were conducted to evaluate some commercial entomopathogenic fungi (EPF) formulations on T. absoluta larvae. The larvae, inside the leaf galleries, were obtained from the established tomato field. Commercial EPF: Metatech® WP [Metarhizium anisopliae (Metschn.) Sorok, Strain FCM Ar 23B3], Beauvitech® WP [Beauveria bassiana (Bals.) Vuill., Strain J25], and Botanigard ES [B. bassiana (Bals.), Strain GHA] were tested in Petri dishes against T. absoluta larvae at a concentration of 108 spores/ml. A synthetic insecticide, imidacloprid was included for comparison as a positive control, while water was used as a negative control. All the tested commercial EPF formulations were pathogenic to T. absoluta larvae in all conducted bioassays. Mortality rates increased with an increase in time (days). However, the insignificant difference was observed in the mortality of T. absoluta larvae treated with the commercial EPF during the first 3 days in all bioassays. Highly significant differences (p < 0.01) in pathogenicity among treatments were observed from the 4th to 6th days after inoculation. Metatech® WP and Beauvitech® WP recorded the highest mortality rates (82.8 and 60.8%) with the LT50 values of 3.9 and 5.2 days, respectively, while imidacloprid caused the least larval mortality. Since the EPF demonstrated high virulence level against the target pest, the efficacy of Metatech® WP and Beauvitech® WP should be advanced to field evaluation to determine their potential as alternatives to the synthetic insecticides.
The roles of rice microRNAs in rice-Magnaporthe oryzae interaction
Authors: Li Yan , Jeyakumar John Martin Jerome , Feng Qin , Zhao Zhi-Xue , Fan Jing , Khaskheli Muhammad Ibrahim , Wang Wen-Ming 
Published: November 2019

AbstractMicroRNAs (miRNAs) are a class of small (20–24 nucleotides (nt) long) non-coding RNAs. One mature miRNA can be transcribed from one or more gene loci known as miRNA genes (MIRs). The transcript of a MIR forms a stem-loop structure that is processed into a 20–24-nt miRNA-5p/−3p duplex by RNase III family endoribonucleases such as Dicer-like1 (DCL1). In turn, the overhang ends of the duplex are methylated by HUA ENHANCER 1 (HEN1), generating stabilized mature miRNAs. The mature miRNAs are loaded onto ARGONAUTE (AGO) proteins, forming a miRNA-induced gene silencing complex (miRISC). Then, the miRISC binds to target sites with sequences complementary to the miRNAs, leading to either cleavage or translational inhibition of the target mRNAs, or methylation of the target sequences, resulting in post-transcriptional and transcriptional gene silencing, respectively. In the past decade, more than 700 miRNAs have been identified in rice, a subset of which have been found to be responsive to the rice blast fungus, Magnaporthe oryzae, or its elicitors. Moreover, members of 10 miRNA families have been found to positively or negatively regulate rice defense against M. oryzae, namely miR160, miR164, miR166, miR167, miR169, miR319, miR396, miR398, miR444 and miR7695. This review summarizes the identification and functional characterization of the miRNAs, which respond to M. oryzae or its elicitors and describes the current understanding of the complicated but well-organized network in the context of rice-M. oryzae interaction.
The launch of BMC Materials
Authors: Manning Harriet E. , Barquinha Pedro , Cui Wenguo , Evans Drew , Kymakis Emmanuel , Wang Haiyan , Zschech Ehrenfried , Martins Rodrigo 
Published: November 2019

AbstractBMC Materials is a new, community-focussed venue for all publishable research across the broad discipline of materials science. It joins the mature and reputable BMC Series of journals, adhering to the same standards of publishing and the ethos of open research set by the Series. Together with its sister journals BMC Chemical Engineering, BMC Biomedical Engineering, BMC Energy and BMC Chemistry, BMC Materials furthers the BMC Series’ expansion into the physical sciences and engineering.
Gene-environment and gene-gene interactions between CHRNA3 rs1051730, XRCC1 rs25487, and ERCC1 rs735482 variants highly elevate the risk of lung cancer
Authors: Ezzeldin Nada , El-Lebedy Dalia , Mohammed Asmaa 
Published: November 2019

Abstract
Background
Gene-gene and gene-environment interactions play an important role in cancer susceptibility. In this work, we studied the association of XRCC1 rs25487, ERCC1 rs735482, and CHRNA3 rs1051730 variants with lung cancer and assessed the modulatory effect of potential interaction between these variants on disease risk.

Results
In this study, 86 primary lung cancer patients and 64 control subjects were genotyped for CHRNA3 rs1051730, XRCC1 rs25487, and ERCC1 rs735482 by real-time PCR. The frequency of the three studied variants was higher among lung cancer patients than in control subjects, but with no statistical significance. ERCC1 rs735482 variant was associated with 6.9-fold increased risk to develop lung cancer among smokers (p = 0.03). Concomitant presence of CHRNA3 and ERCC1 wild alleles was associated with 2.7-fold elevated risk of lung cancer (p < 0.0001), while concomitant presence of CHRNA3 rs1051730 variant allele with ERCC1 wild allele was associated with 20-fold elevated risk (p < 0.000). Concomitant presence of both variants, ERCC1 rs735482 and CHRNA3 rs1051730, was associated with 9.9-fold elevated risk (p < 0.0001). Meanwhile, the concomitant presence of XRCC1 rs25487 with either ERCC1 rs735482 or CHRNA3 rs1051730 or both was not associated with increased risk of the disease.

Conclusion
Our results emphasize the role of gene-gene interaction in the pathogenesis of lung cancer. Large-scale further studies to clarify the underlying mechanisms are needed.
Role of diffusion MR imaging (DWI) and three-dimensional ultrasound (3DUS) in the assessment of placental insufficiency in the gestational hypertension
Authors: Mansour Sahar , Hamed Soha , Sayed Safia , Hosny Shreen 
Published: November 2019

Abstract
Background
DWI is a non-invasive MR modality that is not contrast-based. In the current study, we aimed to evaluate DWI in correlation with 3DUS in the detection of placental insufficiency in high-risk pregnancies complicated with hypertension.
This prospective analysis included 80 pregnancies; 40 hypertensive and 40 controls, gestational age ranged from 22 to 34 weeks. All cases had undergone 3DUS aided by power Doppler scanning and DWI. There is no given contrast. Data were correlated to histopathology.

Results
Doppler US showed a significant relation between RI of the right uterine artery of cases and control (P = 0.014). There was also a positive correlation between the presence of the diastolic notch and RI value. The mean ADC value in the controls was 1.87 ± 0.26 mm2/s, while in hypertensive was 1.36 ± 0.09 mm2/s. In DWI images, there was a significant difference between patients with normal and those with abnormal placental signals (P value = 0.047). Also, there was a significant difference between the measurement of placental volume by MRI and US among cases and controls (P values ≤0.001 and 0.017, respectively).

Conclusion
Diffusion-weighted imaging can detect early subtle findings and signs of placental dysfunction more than detected with 3DUS, so it can add to the diagnostic accuracy of US in imaging of pregnancies at high risk of placental insufficiency.