bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of the Close Collaboration with Parents intervention on parent-infant closeness in NICU&nbsp;
Authors: He Felix , Axelin Anna , Ahlqvist-Björkroth Sari , Raiskila Simo , Löyttyniemi Eliisa , Lehtonen Liisa 
Published: November 2020

Abstract
Background Parent-infant closeness during hospital care of newborns has many benefits for both infants and parents. We developed an educational intervention for neonatal staff, Close Collaboration with Parents, to increase parent-infant closeness during hospital care. The aim of this study was to evaluate the effectiveness of the intervention on parent-infant closeness in nine hospitals in Finland.MethodsParents of hospitalized infants were recruited in the hospitals during 3-month periods before and after the Close Collaboration with Parents intervention. The data were collected using daily Closeness diaries. Mothers and fathers separately filled in the time they spent in the hospital and the time of skin-to-skin contact with their infant during each hospital care day until discharge. Statistical analyses were done using a linear model with covariates.ResultsDiaries were kept before and after the intervention by a total of 170 and 129 mothers and 126 and 84 fathers, respectively. Either parent was present on average 453 minutes per day before the intervention and 620 minutes after the intervention in the neonatal unit. In the adjusted model, the increase was 99 minutes per day (p=0.0007). The infants were in skin-to-skin contact on average 76 minutes per day before the intervention and 114 minutes after the intervention. In the adjusted model, skin-to-skin contact increased by 24 minutes per day (p=0.0405).ConclusionThe Close Collaboration with Parents intervention increased parents’ presence and skin-to-skin contact in nine hospitals. This study suggests that parent-infant closeness may be one mediating factor explaining benefits of parenting interventions.
Genomic regions associated with stripe rust resistance against the Egyptian race revealed by genome-wide association study
Authors: Mohamed Abou-Zeid A. , Mourad Amira M. I. 
Published: November 2020

Abstract
Background: Wheat stripe rust (caused by Puccinia striiformis f. sp. Tritici), is a major disease that causes huge yield damage. New pathogen races appeared in the last few years and caused a broke down in the resistant genotypes. In Egypt, some of the resistant genotypes began to be susceptible to stripe rust in recent years. This situation increases the need to produce new genotypes with durable resistance. Besides, looking for a new resistant source from the available wheat genotypes all over the world help in enhancing the breeding programs. Results: In the recent study, a set of 103-spring wheat genotypes from different fourteen countries were evaluated to their field resistant to stripe rust for two years. These genotypes included 17 Egyptian genotypes from the old and new cultivars. The 103-spring wheat genotypes were reported to be well adapted to the Egyptian environmental conditions. Out of the tested genotypes, eight genotypes from four different countries were found to be resistant in both years. Genotyping was carried out using genotyping-by-sequencing and a set of 26,703 SNPs were used in the genome-wide association study. Five SNP markers, located on chromosomes 2A and 4A, were found to be significantly associated with the resistance in both years. Three gene models associated with disease resistance and underlying these significant SNPs were identified. One immune Iranian genotype, with the highest number of different alleles from the most resistant Egyptian genotypes, was detected. Conclusion: the high variation among the tested genotypes in their resistance to the Egyptian stripe rust race confirming the possible improvement of stripe rust resistance in the Egyptian wheat genotypes. The identified five SNP markers are stable and could be used in marker-assisted selection after validation in different genetic backgrounds. Crossing between the immune Iranian genotype and the Egyptian genotypes will improve stripe rust resistance in Egypt.
Seeking safety: a focus group study of young adults’ cannabis-related attitudes, and behavior in a state with legalized recreational cannabis
Authors: Amroussia Nada , Watanabe Mika , Pearson Jennifer L 
Published: November 2020

Abstract
Background Only July 1, 2017, Nevada became the fifth U.S. state to allow the legal sale of recreational cannabis products for adults ages of 21 and over. This study investigates young adults’ cannabis-related attitudes, perceptions, and behaviors in a state where recreational and medical cannabis use was recently legalized.MethodsWe conducted 8 focus groups stratified by cannabis use (regular users, occasional users, and nonusers) with 32 college students ages 18 to 24. Data were analyzed using the inductive qualitative thematic analysis method. ResultsFour themes emerged during analyses: “sort of legal,” “mitigating harm through legalization,” “Increasing acceptance,” and “seeking safety when purchasing cannabis.” Despite their limited knowledge of cannabis regulation, the majority of the participants supported recreational cannabis legalization from a harm reduction perspective. Most participants did not believe that cannabis legalization had affected their use behavior. However, participants, especially cannabis users, perceived that recreational cannabis legalization created a context where cannabis use was legally, socially, and behaviorally “safer” than in an illegal context, even for those below the legal age of sale. Conclusions Most studies focus on the role of perceived health risk on cannabis use. If there are population-level long-term effects of recreational cannabis legalization on use behavior, findings suggest that they will be mediated by the perceived legal, social, and behavioral risk of using cannabis.
Application of bilateral internal mammary artery with different configurations in coronary artery bypass grafting
Authors: Han Zengqiang , Zhang Guodong , Chen Shenglong , Liu Gang , Chen Yu 
Published: November 2020

Abstract
Background: A large number of studies have shown that BIMA grafting is superior to single internal mammary artery grafting in cardiac function protection and long-term survival after surgery. While, there is still no consensus on how is the best configuration to use BIMA. This study aims to compare intraoperative blood flow, early clinical results and early postoperative patency of different configurations of BIMA.Methods:There were 74 patients who underwent CABGs with bilateral internal mammary artery with different configurations we included. According to the different target territories that RIMA grafted to, the patients were divided into bilateral group (group I) with 20 cases and left group (group II) with 54 cases. Intraoperative blood flow, early clinical results and early postoperative patency of different configurations of BIMA were compared.Results: There was no difference in the early postoperative death and major complications between group I and Group II(P＞0.05). Compared with the LIMA in group II, the LIMA in group I had a slightly higher DF value (76.7±6.2 vs 73.1±6.8, P=0.040). Compared with the RIMA in group II, the RIMA in group I had a slightly higher MGF (51.7±34.4ml/min vs 31.4±21.4ml/min, P=0.024). There was no difference in the other TTFM parameters of LIMA and RIMA between group I and Group II(P＞0.05). Further subgroup analysis revealed that compared with free RIMA in group II, in situ RIMA had a higher DF value(71.4±7.8 vs 61.8±18.1 ,P=0.025).The PI of LIMA in free RIMA subgroup was higher than the PI of LIMA in in-situ RIMA subgroup(3.0±1.6 vs 2.1±1.0,P=0.018). The results of early postoperative CTA examination showed that all IMAs grafts were completely patent.ConclusionsThe use of BIMA for CABG is safe and efficacious, RIMA used in right coronary artery received more satisfactory graft flow. BIMA with no stenosis and occlusion in the early stage, therefore is the ideal and stable coronary bypass graft.
Mutations in SARS-CoV-2 spike protein and RNA polymerase complex are associated with COVID-19 mortality risk
Authors: Lange Christoph , Hahn Georg , Wu Chloe , Lee Sanghun , Hecker Julian , Lutz Sharon , Haneuse Sebastien , Qiao Dandi , Cho Michael , Randolph Adrienne , Laird Nan , Weiss Scott , Silverman Edwin , Ribbeck Katharina 
Published: November 2020

Abstract
SARS-CoV-2 mortality has been extensively studied in relationship to a patient's predisposition to the disease. However, how sequence variations in the SARS-CoV-2 genome affect mortality is not understood. To address this issue, we used a whole-genome sequencing (WGS) association study to directly link death of SARS-CoV-2 patients with sequence variation in the viral genome. Specifically, we analyzed 3,626 single stranded RNA-genomes of SARS-CoV-2 patients in the GISAID database (Elbe and Buckland-Merrett, 2017; Shu and McCauley, 2017) with reported patient’s health status from COVID-19, i.e. deceased versus non-deceased. In total, evaluating 28,492 loci of the viral genome for association with patient/host mortality, two loci, 12,053bp and 25,088bp, achieved genome-wide significance (p-values of 1.24e-12, and 1.24e-26, respectively). Mutations at 25,088bp occur in the S2 subunit of the SARS-CoV-2 spike protein, which plays a key role in viral entry of target host cells. Additionally, mutations at 12,053bp are within the ORF1ab gene, in a region encoding for the protein nsp7, which is necessary to form the RNA polymerase complex responsible for viral replication and transcription. Both mutations altered amino acid coding sequences, potentially imposing structural changes that could enhance viral infectivity and symptom severity, and may be important to consider as targets for therapeutic development.
Review—Recent Advancements in Electrochemical Biosensors for Alzheimer’s Disease Biomarkers Detection
Authors: Mikuła Edyta 
Published: November 2020

Background:
It is estimated that the average time between the diagnosis of Alzheimer’s disease (AD) and the patient’s death is 5-9 years. Therefore, both the initial phase of the disease and the preclinical state can be included in the critical period in disease diagnosis. Accordingly, a huge progress has recently been observed in biomarker research to identify

risk factors for dementia in older people with normal cognitive functions and mild cognitive impairments.

Methods:
Electrochemical biosensors are excellent analytical tools that are used in the detection of AD biomarkers as they
are easy to use, portable, and can do analysis in real time.

Results:
This review presents the analytical techniques currently used to determine AD biomarkers in terms of their advantages and disadvantages; the most important clinical biomarkers of AD and their role in the disease. All recently used biorecognition molecules in electrochemical biosensor development, i.e. receptor protein, antibodies, aptamers and nucleic acids are summarized for the first time. Novel electrochemical biosensors for AD biomarker detection, as ideal analytical platforms for point-of-care diagnostics, are also reviewed.

Conclusions:
The article focuses on various strategies of biosensor chemical surface modifications to immobilize biorecognition molecules, enabling specific, quantitative AD biomarker detection in synthetic and clinical samples. In addition, this
is the first review that presents innovative single-platform systems for simultaneous detection of multiple biomarkers and
other important AD-associated biological species based on electrochemical techniques. The importance of these platforms in
disease diagnosis is discussed.
COVID-19 in People with Diabetes: Perspectives from Saudi Arabia
Authors: Robert Asirvatham Alwin , Al Dawish Mohamed Abdulaziz 
Published: November 2020

Abstract::
Coronavirus disease 2019 (COVID-19) and diabetes have major impacts on the health
of the population across the world. Since the beginning of the COVID-19 pandemic, people with diabetes
have been identified to be more vulnerable to infection and at greater risk for hospitalization.
As diabetes is one of the major health issues in Saudi Arabia, the current study describes the
perspectives of COVID-19 in people with diabetes and the steps taken by the government to minimize
its impact. Most patients with COVID-19 in Saudi Arabia experience mild illness, while people
with diabetes are at increased risk of disease severity and mortality. The government of Saudi
Arabia has taken several measures to control and mitigate the effect of the pandemic, as the Saudi
population is gradually returning back to normal life. However, currently, there are limited studies
from Saudi Arabia on COVID-19 among people with diabetes and the effectiveness of interventions.
We emphasize the necessity for comprehensive research, which would provide a better understanding
of the incidence of COVID-19 and its association with diabetes to develop evidence-
based programs and policies in the country.
Synthesis, Molecular Docking and Biological Activity Evaluation of Alkoxy Substituted Chalcone Derivatives: Potential Apoptosis Inducing Agent on MCF-7 Cells
Authors: Khairul Wan M. , Hashim Fatimah , Mohammed Mas , Shah Nurul S.M.N. , Johari Syed A.T.T. , Rahamathullah Rafizah , Daud Adibah I. , Ma Nyuk L. 
Published: November 2020

Introduction:
In this contribution, a series of alkoxy substituted chalcones were successfully
designed, synthesized, spectroscopically characterized and evaluated for their cytotoxicity potential in inhibiting
the growth of MCF-7 cells.

Objective::
In order to investigate the influence between electron density in conjugated π-systems and biological
activities, different withdrawing substituents, namely Nitro (NO2), Cyano (C≡N) and trifluoromethyl (CF3) were
introduced in the chalcone-based molecular system.

Methods:
All the derivatives were then tested on MCF-7 cell line using the fluorescence microscopy-based
cytotoxicity analyses.

Results:
The preliminary findings showed that both –NO2 and –CF3 substituents revealed their potential to inhibit
the growth of MCF-7 with IC50 values of 14.75 and 13.75 μg/ml, respectively. In addition, the morphological
changes of MCF-7 cells were observed in response to alkoxy substituted chalcone treatment through an induction
of apoptosis pathway with cell blebbing, phosphatidylserine exposure and autophagic activity with acidification
of lysosomal structure. Intermolecular interaction based on in silico investigation on nitro, trifluoromethyl and
cyano based chalcones exhibited several types of interactions with tumor necrosis factor receptor (PDB: 1EXT)
protein and high hydrogen bond in the molecule-receptor interaction have given significant impact towards their
toxicity on MCF-7 cells.

Conclusion:
Significantly, these types of chalcones exhibited ideal and high potential to be further developed as
anti-cancer agents.
New Approaches for the Synthesis of Fused Thiophene, Pyrazole, Pyran and Pyridine Derivatives with Anti-Proliferative Together with c-Met Kinase and Prostate Cancer Cell Inhibitions
Authors: Mohareb Rafat M. , Milad Yara R. , El-Ansary Reem A. 
Published: November 2020

Background:
Recently multi-component reactions producing pyran and pyridine derivatives acquired a
special attention due to their wide range of pharmacological activities, especially therapeutic activities. Through
the market, it was found that many pharmacological drugs containing the pyran and pyridine nucleus were
known.

Objective:
We are aiming in this work to synthesize target molecules possess not only anti-tumor activities but
also kinase inhibitors. The target molecules were obtained starting from cyclohexane-1,3-dione followed by its
heterocyclization reactions to produce anticancer target molecules.

Methods:
This work demonstrated multi-component reactions of cyclohexan-1,3-dione with aromatic aldehydes
and diethylmalonate using triethylamine as a catalyst to give the 7,8-dihydro-4H-chromen-5(6H)-one derivatives
4a-c. The reaction of compounds 4a-c with either of hydrazine hydrate of phenylhydrazine gave the
chromeno[2,3-c]pyrazole derivatives 5a-f, respectively. In addition, further heterocyclization reactions were
adopted to give the chromeno[3,2-d]isoxazole, chromene-3-carboxamide derivatives. Moreover, the multicomponent
reaction of cyclohexan-1,3-dione (1) with either of aromatic aldehydes and diethylmalonate using a
catalytic amount of ammonium acetate gave the 1,4,5,6,7,8-hexahydroquinoline derivatives 13a-c. The antiproliferative
activities of the synthesized compounds toward the six cancer cell lines namely A549, H460, HT-
29, MKN-45, U87MG, and SMMC-7721 were studied. In addition, the c-Met enzymatic activities and inhibition
toward the prostate cancer cell PC-3 were measured.

Results:
Anti-proliferative evaluations, c-Met enzymatic activities and inhibition toward the prostate cancer cell
PC-3 were measured, and the results obtained in most cases indicated that the presence of electronegative Cl
group through the molecule favour the inhibitions.

Conclusion:
The compounds with high anti-proliferative activity towards the cancer cell lines were 4a, 4b, 6d,
6e, 6f, 10e, 10f, 12c, 14e, 14f, 15c, 16d, 16e, 16f, 19c and 20c. Compounds 4b, 6c, 6d, 8b, 10c, 10d, 12b, 13b,
14c, 14d, 15b, 16c, 16d, 17b, 17c, 19b, 20b and 20c exhibited high potency against c-Met kinase and
compounds 4a, 4b, 6b, 6c, 6d, 6f, 8b, 8c, 10c, 10d, 10e, 12b, 12c, 13a, 13b, 13c, 14c, 14d, 14e, 14f, 15b, 15c,
16b, 16c, 16d, 17b, 17c, 19c, 19d, 20a, 20b and 20c displayed high inhibitions toward PC-3 cell line.
Panax notoginseng Saponins Promote Cell Death and Chemosensitivity in Pancreatic Cancer through the Apoptosis and Autophagy Pathways
Authors: Yao Li-Chao , Wu Lun , Wang Wei , Zhai Lu-Lu , Ye Lin , Xiang Feng , Tang Zhi-Gang 
Published: November 2020

Background:
Panax notoginseng Saponins (PNS) is used as a traditional Chinese medicine for
ischemic stroke and cardiovascular disease; it has been proven to possess anticancer activity recently.

Objective:
In this study, we aimed to explore the curative anticancer effect and potential mechanisms of PNS in
pancreatic cancer cells.

Methods:
Pancreatic cancer Miapaca2 and PANC-1 cells were treated with PNS and Gemcitabine (Gem), respectively. Then the cell
viability was assessed by CCK-8 assay, cell proliferation was tested by colony formation assay and EdU cell proliferation assay, cell
migration and invasiveness were tested by wound healing assay and transwell assay respectively, and cell apoptosis was detected by flow
cytometry. Finally, we detected the expression levels of proteins related to migration, apoptosis and autophagy through Western blotting.

Results:
PNS not only inhibited the proliferation, migration, invasion and autophagy of Miapaca2 and PANC-1
cells, but also induced apoptosis and promoted chemosensitivity of pancreatic cancer cells to Gem.

Conclusion:
PNS may exhibit cytotoxicity and increase chemosensitivity of pancreatic cancer cells to Gem by
inhibiting autophagy and inducing apoptosis, providing a new strategy and potential treatment option for
pancreatic cancer.
Model manajemen Madrasah Aliyah Pembangunan Universitas Islam Negeri (UIN) Jakarta
Authors: Achmad Widodo Syukri Fathudin , Sudrajat Ajat , Sugiyono Sugiyono 
Published: November 2020

The article aims to see the Management Model of Madrasah Aliyah Laboratorium from the aspects of planning, organizing, implementing, and monitoring. At Madrasah Aliyah Pembangunan (MAP) UIN Jakarta. The research method used is qualitative. The results showed the Madrasah Aliyah Laboratory management model consists of the first three components: the organizational structure of the Madrasah Aliyah Laboratory, including management (Director) and Madrasah Implementer (Madrasah Head), the second existing culture (culture). in Madrasah Aliyah Laboratory including academic and non-academic including custom curriculum, reading habits, English-Arabic days and the three figures who play a role in Madrasah Aliyah Laboratorium, both head of a madrasah, teachers, and madrasah committee
Development and Validation of an Automated Algorithm to Detect Atrial Fibrillation Within Stored Intensive Care Unit Continuous Electrocardiographic Data: Observational Study
Authors: Walkey Allan J , Bashar Syed K , Hossain Md Billal , Ding Eric , Albuquerque Daniella , Winter Michael , Chon Ki H , McManus David D 
Published: November 2020

Background
Atrial fibrillation (AF) is the most common arrhythmia during critical illness, representing a sepsis-defining cardiac dysfunction associated with adverse outcomes. Large burdens of premature beats and noisy signal during sepsis may pose unique challenges to automated AF detection.

Objective
The objective of this study is to develop and validate an automated algorithm to accurately identify AF within electronic health care data among critically ill patients with sepsis.

Methods
This is a retrospective cohort study of patients hospitalized with sepsis identified from Medical Information Mart for Intensive Care (MIMIC III) electronic health data with linked electrocardiographic (ECG) telemetry waveforms. Within 3 separate cohorts of 50 patients, we iteratively developed and validated an automated algorithm that identifies ECG signals, removes noise, and identifies irregular rhythm and premature beats in order to identify AF. We compared the automated algorithm to current methods of AF identification in large databases, including ICD-9 (International Classification of Diseases, 9th edition) codes and hourly nurse annotation of heart rhythm. Methods of AF identification were tested against gold-standard manual ECG review.

Results
AF detection algorithms that did not differentiate AF from premature atrial and ventricular beats performed modestly, with 76% (95% CI 61%-87%) accuracy. Performance improved (P=.02) with the addition of premature beat detection (validation set accuracy: 94% [95% CI 83%-99%]). Median time between automated and manual detection of AF onset was 30 minutes (25th-75th percentile 0-208 minutes). The accuracy of ICD-9 codes (68%; P=.002 vs automated algorithm) and nurse charting (80%; P=.02 vs algorithm) was lower than that of the automated algorithm.

Conclusions
An automated algorithm using telemetry ECG data can feasibly and accurately detect AF among critically ill patients with sepsis, and represents an improvement in AF detection within large databases.