bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nomogram Based on Preoperative Clinical Characteristics to Predict the Risk of Lymph Node Metastasis in Hepatocellular Carcinoma
Authors: Wei Rongyu , Li Shuqun , Ren Liying , Yu Junxiong , Liao Weijia 
Published: November 2020

Abstract
Background: There are limitations in judging the occurrence of lymph node metastasis (LNM) in hepatocellular carcinoma (HCC) before surgery. The purpose of this study was to establish a preoperative nomogram for predicting the risk of LNM in HCC and to explore its clinical utility.Methods: A total of 195 HCC patients undergoing radical hepatectomy were retrospectively analyzed. According to the presence or absence of LNM, the patients were divided into two groups, and the clinical characteristics of the two groups were compared. Risk factors for LNM were assessed based on logistic regression, and a nomogram was established. The receiver operating characteristic (ROC) curve was used to calculate area under the curve (AUC) of the logistic regression model, and the predictive accuracy of the nomogram was evaluated by the concordance index (C-index). The clinical efficacy of the nomogram was detected by decision curve analysis (DCA).Results: Logistic analysis revealed hepatitis B surface antigen (HBsAg) (HR = 3.50, 95% CI, 1.30-9.42, P = 0.013), globulin (HR = 2.46, 95% CI, 1.05-5.75, P = 0.039), neutrophil to lymphocyte ratio (NLR) (HR = 7.64, 95% CI, 3.22-18.11, P < 0.001) and tumor size (HR = 3.86, 95% CI, 1.26-11.88 P = 0.018) were independent risk factors for lymph node metastasis in HCC. The nomogram was established based on the above 4 variables, and the AUC was 0.835 (95% CI, 0.780-0.890). The calibration curve showed that the model has good predictive ability, and DCA indicates good predictive effect.Conclusions: The nomogram established by analyzing the preoperative clinical characteristics is a simple tool that can predict the risk of lymph node metastasis in HCC patients and guide clinicians to make better clinical decisions.
Successful Treatment For a Young Patient With Multiple Synchronous Breast And Lung Carcinoma: A Case Report
Authors: Zhao Mengfan , Bi Lusha , Wu Bin 
Published: November 2020

Abstract
Introduction Reports of synchronous multiple primary cancers in patients with breast cancer have recently been increasing because of progress in radiographic diagnostic techniques. Multiple primary cancers in patients with breast cancer mainly occur in the lung and endometrium. Case report We report a 37-year-old female patient diagnosed with synchronous double primary cancers, namely invasive ductal carcinoma of the left breast and invasive carcinoma of inferior lobe of the right lung based on the pathological examination. After MDT, we gave her both lung and breast surgery then adjuvantchemothrapy(AC-T) and EGFR-TKI (Gefitinib). We followed this patient until October, 2020, the patient was healthy and with no clinical or radiologic signs of recurrent or metastatic disease at the time of this writing.Discuss The pathogenesis of MPCs is still unclear, which is closely related to many factors, such as genetic, immune, iatrogenic, endocrine, lifestyle. The diagnosis depends on chest CT and Pathology examination. And there is no standard treatment guideline for MPCs. The specific therapy plan should be made after MDT discussion.Conclusion Multiple synchronous breast and lung adenocarcinoma in one patient dose not like metastatic carcinoma. If the treatment is in time, the patient can also get a good prognosis.
Distinct Landscapes of Genetic and Epigenetic Alterations of E2F Family Genes Between Esophageal Squamous Cell Carcinoma and Esophageal Adenocarcinoma
Authors: Zheng Guo-Liang , Zhang Guo-Jun , Wu Tian-Yi , Zhao Yan , Zheng Zhi-Chao 
Published: November 2020

Abstract
Background/Aims: Esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC) are primarily driven by different genetic changes. The E2F transcription factors (E2Fs) are involved in various malignancies; yet little is known about their roles in ESCC and EAC. This study aimed to investigate genetic and epigenetic alterations of the E2F family genes in ESCC and EAC, and to examine whether the differential changes are associated with the distinct mechanisms. Materials and Methods: mRNA and methylation levels of E2F family genes in ESCC and EAC were retrieved from the database UALCAN. The cBioPortal for Cancer Genomics and Search Tool for the Retrieval of Interacting Genes (STRING) database were used to analyze genetic mutations and interaction networks for E2Fs, respectively.Results: The genetic alteration patterns of E2Fs were different between ESCC and EAC. Furthermore, differences in methylation levels, CNV, and mutation frequency in certain E2F family genes were observed between ESCC and EAC. Moreover, E2Fs were co-expressed and interacted with proteins involved in the N6-methyladenosine (m6A) and histone modifications in EC. Additionally, Kaplan–Meier analysis revealed significant lower E2F1, E2F3, E2F5, E2F7, and E2F8 mRNA levels in association with better prognosis for EAC; while lower E2F1, E2F3, E2F6, E2F7, and E2F8 mRNA levels indicated poorer outcome for ESCC. Conclusions: The different patterns of genetic and epigenetic alterations in the E2F family genes between ESCC and EAC are likely to be associated with different mechanisms. Certain E2Fs hold promise as biomarkers for differential diagnosis of EAC and ESCC, and for prognostic prediction.
Ring Finger Protein 180 Suppresses Cell Proliferation and Energy Metabolism of Non-Small Cell Lung Cancer Through Down-Regulating C-myc
Authors: Ding Yi , Lu Yi , Xie Xinjie , Cao Lei , Zheng Shiying 
Published: November 2020

Abstract
BackgroundNon-small cell lung cancer (NSCLC) causes a great number of cancer-related mortality worldwide, but the biomarkers for prognosis of NSCLC are scarce because of their inconsistent efficiency. Proteins containing RING finger domain are the key mediator for ubiquitination, which controls cell cycle and regulates tumor progression. Ring Finger Protein 180 (RNF180) has been reported to suppress gastric cancer, whereas its function in NSCLC is still unclear. In this study, the association between RNF180 expression and NSCLC as well as the effect of RNF180 in cellular proliferation and metabolism of NSCLC were investigated. MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical staining were performed to analyse RNF180 levels. Moreover, RNA interference (RNAi) and lentiviral-mediated vector transfections were performed to silence and overexpress RNF180. Further, Cell Counting Kit-8 (CCK-8) was used to assess its biological function in cell proliferation. A xenograft model was used to examine RNF180 function in vivo. ResultsWe found that the expression of RNF180 was decreased in NSCLC tissues, and its expression was positively correlated with the survival rate of NSCLC patients. Furthermore, the overexpression of RNF180 in NSCLC cells suppressed their proliferation, glycolytic activities, and mitochondrial respiration in vitro, and it restricted the tumorigenicity in mice. In addition, RNF180 knockdown promoted NSCLC cell proliferation and energy metabolism, whereas these promotive effects were counteracted by C-myc inhibitor. The underlying anti-NSCLC mechanism of RNF180 involved in the down-regulation of C-myc through ubiquitin-dependent degradation, and subsequently reduced C-myc downstream: lactate dehydrogenase-A (LDHA) and hexokinase-2 (HK2). ConclusionsThese results firstly indicated the anti-tumor properties of RNF180 and its significant correlation with NSCLC, which endorses RNF180 with the potential as efficient prognostic biomarker for tumor recurrence in NSCLC.
Leptomeningeal cells induce&nbsp;synaptic failure via Cathepsin B-mediated IL-1β production after Porphyromonas gingivalis infection
Authors: Huang Wanyi , Zeng Fan , Gu Yebo , Jiang Muzhou , Zhang Xinwen , Yan Xu , Kadowaki Tomoko , Mizutani Shinsuke , Kashiwazaki Haruhiko , Ni Junjun , Wu Zhou 
Published: November 2020

Abstract
Background: Synaptic failure is the earliest sign before Alzheimer’s disease (AD) onset and closely associated with cognitive decline. Clinical studies have shown that periodontitis is positively correlated with both the onset and progression of AD, and preclinical studies have shown that Porphyromonas gingivalis (P. gingivalis) and its virulence factors induced memory decline in mice. However, the mechanisms underlying the involvement of P. gingivalis in memory decline remain unclear.Methods: Fifteen-month-old female C57/BL6J mice were intraperitoneally injected with P. gingivalislearning and memory were evaluated by step through passive avoidance tests. Immunofluorescent staining was used to examine the expression of IL-1β and synaptic markers. Primary leptomeningeal cells and primary cortical neurons were used to evaluate the its effects on synaptic generation and plasticity. The expression of related molecules was examined by Q-PCR and Western blotting. Pharmacological and genetic approaches were used to explore the roles of leptomeningeal cells in synaptic changes after P. gingivalis infection. Results: P. gingivalis infection induced the increased expression of IL-1β in leptomeninges and decreased expression of synaptophysin (SYP) in the cortex proximity of the leptomeninges, which was accompanied by memory decline in middle-aged mice. NLRP3 inflammasome activation was involved in augmenting the IL-1β secretion by primary leptomeningeal cells after P. gingivalis infection. Cathepsin B (CatB) mediated the activation of both NLRP3 inflammasome and NF-κB in P. gingivalis-infected primary leptomeningeal cells. In contrast, P. gingivalis-infected leptomeningeal cells induced an IL-1β-dependent decrease in pre- and post-synaptic molecules in primary cortical neurons, as determined by the pharmacological blockage of the IL-1 receptor. P. gingivalis-infected leptomeningeal cells also induced the IL-1β-dependent suppression of BDNF signaling in cultured N2a neurons. Furthermore, propolis produced by honeybees, suppressed the expression of IL-1β but increased that of BDNF in P. gingivalis-infected leptomeningeal cells. Conclusion: The CatB-mediated IL-1β production was augmented in leptomeningeal cells, resulting in synaptic failure and blockage of BDNF signaling in neurons during P. gingivalis infection. These findings highlight a new mechanism underlying the involvement of periodontitis in AD initiation and suggest that CatB may be an early intervention therapeutic target for delaying the onset of AD during P. gingivalis infection.
Effect of low-sugar and High-dietary Fiber Noodles on Glycemic Response in Healthy Humans: A Cross-over Trial
Authors: Kadokura Kazunari , Tomita Tsuyoshi , Hata Sachiyo , Kamata Kazuhiko , Suruga Kohei 
Published: November 2020

Abstract
Background: Low-carbohydrate diets are popular nutritional therapeutics based on recommendations of the American Diabetes Association (2013). In previous reports, we suggested a new diet-intake restriction method using low-sugar, high-dietary fiber noodles containing okara and konjac and reported their effect on blood glucose levels in healthy animals and animal models of type 2 diabetes. This study aimed to compare the effect of low-sugar, high-dietary fiber noodles with that of normal wheat noodles on glycemic response in healthy adult men.Methods: The study was a randomized, cross-over design to investigate the effects of normal wheat noodles versus low-sugar, high-dietary fiber noodles on glucose and insulin responses at 120 min. Plasma glucose and insulin levels were measured at 0, 15, 30, 45, 60, and 120 min after intake of normal wheat noodles or low-sugar, high-dietary fiber noodles.Results: No abnormalities and side effects were observed in the low-sugar, high-dietary fiber noodles group. Additionally, in men who consumed low-sugar, high-dietary fiber noodles, the increase in postprandial blood glucose levels and insulin levels was significantly suppressed compared with that in men who consumed normal wheat noodles. Conclusions: To the best of our knowledge, this is the first report on the effectiveness of low-sugar, high-dietary fiber noodles on postprandial blood glucose levels in healthy humans; our findings also suggest that such type of noodles might be beneficial for patients with diabetes or celiac disease who consume noodles.Trial registration: UMIN000023986
A Deep Learning Based Methods for Forecasting Gold Price with Respect to Pandemics
Authors: Khani Mahtab Mohtasham , Vahidnia Sahand , Abbasi Alireza 
Published: November 2020

Abstract
The spread of COVID-19 in the world had a devastating impact on the world economy, trade relations, and globalization. As the pandemic advances and new potential pandemics are on the horizon, a precise analysis of recent fluctuations of trade becomes necessary for international decisions and controlling the world in similar crisis. The COVID-19 pandemic made a new pattern of trade in the world and affected how businesses work and trade with each other. It means that every potential pandemic or any unprecedented event in the world can change the market rules. This research develops a novel model to have a proper estimation of the stock market values with respect to COVID-19 dataset using long short-term memory networks (LSTM).The nature of the features in each pandemic is totally different, thus, prediction results for a pandemic by a specific model cannot be applied to other pandemics. Hence, recognising and extracting the features which affect the pandemic is in the highest priorities. In this study, we develop a framework, providing a better understanding of the features and feature selection. This study is based on a preliminary analysis of such features for enhancing forecasting models' performance against fluctuations in the market.Our forecasts are based on the market value data and COVID-19 pandemic daily time-series data (i.e. the number of new cases). In this study, we selected Gold price as a base for our forecasting task which can be replaced by any other markets. We have applied Convolutional Neural Networks (CNN) LSTM, Vector Out-put Sequence LSTM, Bidirectional LSTM, and Encoder-Decoder LSTM on our dataset and our results achieved an MSE of 6.0e-4, 8.0e-4, and 2.0e-3 on the validation set respectfully for one day, two days, and 30 days predictions in advance which is outperforming other proposed method in the literature.
Kawasaki disease in pediatric&nbsp;isolation ward&nbsp;during COVID-19 pandemic
Authors: Chen Xia , Chen Xiuzhen , Shu Junhua , Huang Yang , Wu Yabin 
Published: November 2020

Abstract
Background: To our knowledge, a link between an emergence of Kawasaki disease and the ongoing COVID-19 pandemic has not been reported in China. Methods: This study retrospectively analyzed the clinical data of Kawasaki patients admitted to Maternal and Child Health Hospital of Hubei Province from January 1, 2015 to July 31, 2020. Kawasaki patients admitted to the isolation ward from January 1, 2020 to July 31, 2020 as the current cohort and Kawasaki patients admitted to the general wards from January 1, 2015 to December 31, 2019 as the historical cohort were analyzed.Results: The current cohort comprised 15 patients (10 boys and 5 girls, median age 11 months) without severe form. SARS-CoV-2 nucleic acid test was performed in 13 cases and SARS-CoV-2 antibodies (IgM and IgG) was conducted in 11 cases, but no positive results were found. The historical cohort included 89 patients (54 boys and 35 girls, median age 15 months). Comparison between the historical cohort and the current cohort demonstrated that there were no significant differences in age, sex, clinical manifestations, blood routine examination, blood biochemistry, cardiac ultrasound and treatment, but the incidence and the abnormal chest imaging in the current cohort were significantly higher than those in the historical cohort. Conclusion: This study suggested that there may be an association between the emergence of Kawasaki disease with non-severe form and COVID-19 pandemic in Wuhan.
Inhibitory effects of iron depletion plus eribulin on the breast cancer microenvironment
Authors: Goto Wataru , Kashiwagi Shinichiro , Asano Yuka , Takada Koji , Morisaki Tamami , Takahashi Katsuyuki , Fujita Hisakazu , Shibutani Masatsune , Amano Ryosuke , Takashima Tsutomu , Tomita Shuhei , Hirakawa Kosei , Ohira Masaichi 
Published: November 2020

Abstract
Background: Iron is required for the proliferation of cancer cells, and its depletion suppresses tumor growth. Eribulin mesylate (eribulin), a non-taxane microtubule inhibitor, disrupts the tumor microenvironment via vascular remodeling and obstruction of the epithelial-mesenchymal transition (EMT). Herein, we investigated the effects of the iron chelator on tumor-related properties of breast cancer cells and the effects of iron chelator plus eribulin on tumor growth in vivo.Methods: Two triple-negative breast cancer (TNBC) cell lines, MDA-MB-231 and BT-549, and one hormone-receptor positive breast cancer cell line, MCF-7, were used in our study. Cell proliferation, cell migration, cell cycle position, and gene expression were analyzed via MTT assays, wound-healing assays, flow cytometry, and quantitative real-time-polymerase chain reaction, respectively. For the in vivo experiments, mice with breast cancer xenografts were treated with the inhibitors, alone or together, and tumor volume was determined.Results: Iron chelator inhibited breast cancer cell proliferation and decreased the proportion of S-phase cells. Conversely, it induced hypoxia, angiogenesis, EMT, and immune checkpoints, as determined by quantifying the expression of marker mRNAs in MDA-MB-231 and MCF-7 cells. Eribulin suppressed the expression of the hypoxia and EMT related marker mRNAs in the presence of iron chelator. Iron chelator plus eribulin inhibited tumor growth in vivo to a greater extent than did either inhibitor alone.Conclusions: Although iron chelator induces oncogenic events (hypoxia, angiogenesis, EMT, and immune checkpoints), it may be an effective treatment for breast cancer when administered in combination with eribulin.
Effect of Streptococcus anginosus on biological response of tongue squamous cell carcinoma cells&nbsp;
Authors: Xu Yuan , Jia Yuhuan , Chen Liang , Gao Jing , Yang Deqin 
Published: November 2020

Abstract
Background: Streptococcus anginosus(S.anginosus) was reported increased in oral squamous cell carcinoma(OSCC) tissue. The aim of this study was to investigate the response of oral cancer cells in the biological characteristics evoked by the S.anginosus and investigate its potential mechanisms.Methods: The growth curve and concentration standard curve of S.anginosus were determined , and a series of concentrations of S.anginosus supernatant were applied to OSCC cell lines SCC15,then selected an optimal time and concentration by CCK-8 assay. Then autophagic response, proliferative activity, cell cycle and apoptosis, invasion and migration abilities were evaluated in SCC15.Results: The results showed that when the ratio of S.anginosus supernatant to cell culture medium was 1:1 and the co-culture time was 16h, there was a most significant inhibited effect on SCC15; Furthermore, S.anginosus supernatant can up-regulate the autophagy activity of SCC15, then, the ability of proliferation, migration and invasion was observed inhibited obviously. Compared with the control groups, the cell cycle showed G1 arrest, S and G2 / M phases decreased, and the percentage of apoptotic cells relatively increased(P<0.05).Conclusion: S.anginosus can reduce the proliferation, migration and invasion of SCC15 cells and promote cell apoptosis; Moreover, autophagy may be one of the mechanisms in this process.
Using augmented reality technology for balance training in the elderly. A feasibility pilot study
Authors: Blomqvist Sven , Seipel Stefen , Engström Maria 
Published: November 2020

Abstract
Background: Impaired balance leading to falls is common in the elderly, and there is strong evidence that balance training reduces falls and increases independence. Reduced resources in health care will result in fewer people getting help with rehabilitation training. In this regard, the new technology augmented reality (AR) could be helpful. With AR, the elderly can receive help with instructions and get feedback on their progression in balance training. The purpose of this pilot study was to examine the feasibility of using AR-based visual-interactive tools in balance training of the elderly. Methods: Seven elderly participants (66-88 years old) with impaired balance trained under supervision of a physiotherapist twice a week for six weeks using AR-based visual-interactive guidance, which was facilitated through a Microsoft HoloLens holographic display. Afterwards, participants and physiotherapists were interviewed about the new technology and their experience of the training. Also, fear of falling and balance ability were measured before and after training. Results: Five participants experienced the new technology as positive in terms of increased motivation and feedback. Experiences were mixed regarding the physical and technical aspects of the HoloLens and the design of the HoloLens application. Participants also described issues that needed to be further improved, for example, the training program was difficult and monotonous. Further, the HoloLens hardware was felt to be heavy, the application's menu was difficult to control with different hand manoeuvres, and the calibration took a long time. Suggestions for improvements were described. Results of the balance tests and self-assessment instruments indicated no improvements in balance performance after AR training. Conclusions: The study showed that training with the new technology is, to some extent, feasible for the elderly, but needs further development. Also, the technology seemed to stimulate increased motivation, which is a prerequisite for adherence to training. However, the new technology and training requires further development and testing in a larger context.