bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioinformatics and Expression Pattern Analysis of Soybean Fatty Acid Desaturase Family Gene
Authors: Wu Nan , Jiang Long , Yu Xiaoming , Yang Xiangbo , Chen Dianyuan , Yuan Minghao 
Published: December 2019

Abstract
To clarify the homology and structural differences of seven genes in the soybean fatty acid desaturase 2 (FAD2) family and the relationship between the expression level of each gene and oleic acid content at different stages of grain development, the seven genes in this family were studied through informatics analysis and evaluation of expression patterns. Amino acid sequence analysis of the seven FAD2 genes in soybean showed that the genes were located on 6 chromosomes, and the number of amino acids encoded by all the genes ranged between 216 and 387. Isoelectric point analysis showed that the proteins were alkaline. Phylogenetic tree comparison results indicated that the soybean FAD2 genes have higher homology with the Arabidopsis thaliana gene and belong to the same dicotyledon, while GmFAD2-2 and GmFAD2-2a have the highest homology, and GmFAD2-1 and GmFAD2-1b have the same homology, the highest source and less homology with other soybean FAD2 genes, and their function is expected to be slightly different. Transmembrane prediction of each gene in the family showed that GmFAD2-2b contains two transmembrane regions. The remaining genes contain 3-6 different transmembrane structures, all of which are hydrophilic. The amino acid sequences were aligned, and the results showed that the genes in this family contain histidine clusters at the same position, but in GmFAD2-2b, the histidine cluster composition is different from that in other genes. According to the gene 5’ UTR intron cis-regulatory element analysis, the gene contains hormone response elements, light response elements, meristem response elements, and participates in low temperature as a cis-acting element. By analysing the expression patterns of genes in the FAD2 family under low temperature stress, it was found that GmFAD2-2 and GmFAD2-1b exhibited seed-specific expression, while the other genes were constitutively expressed. The genes were up-regulated under low temperature stress. To adapt to low temperature, plants produced more unsaturated fatty acids, and the expression of genes was different at different treatment times. In four soybean genotypes, "Jike Soybean 20", "Ji Midou 3", "Jike Midou No. 1", and "Jike Fresh Bean 1", the soybean FAD content at different stages of grain development was determined, and content was found to increase, then decrease, and finally stabilize. Correlation analysis showed that there was a negative correlation between gene expression and oleic acid content, and the correlation coefficients for GmFAD2-1 and GmFAD2-2b were the largest, showing a significant negative correlation.
Comparative effectiveness of surgery and radiotherapy for survival of clinically localized prostate cancer patients: A population-based coarsened exact matching retrospective cohort study
Authors: Yasui Masato , Sakaguchi Masahiko , Jikuya Ryousuke , Tsutsumi Sohgo , Tatenuma Tomoyuki , Noguchi Go , Umemoto Susumu , Katayama Kayako , Narimatsu Hiroto , Ueumura Hiroji , Kishida Takeshi 
Published: December 2019

Abstract
Background. Radical prostatectomy and radiotherapy are currently the main treatment options for localized prostate cancer. However, not yet a large cohort study of comparison between surgery and radiation has been investigated in Japan nor Asia. Objective of this study was to compare the survival outcome between surgery and radiotherapy among patients with clinically localized prostate cancer and in the elderly and young patients. Methods. We retrospectively evaluated survival outcomes of localized prostate cancer patients (age at diagnosis ≤79 years, cT1-3) initially treated with surgery or radiotherapy. Data were collected from the population-based cancer registry of Kanagawa Prefecture, Japan. A 1:1 coarsened exact matching of age at diagnosis, clinical T stage, and cancer differentiation was made between the two treatment groups. Patients were also categorized into two groups by age at a cut-off of 70 years for analysis. Results. The cohort comprised 4,810 patients aged 50-79 years. No significant difference in CSS was observed between the two groups (p=0.612), but the surgery group had significantly better prognosis in OS (p=0.004). When stratified for age, similar tendencies were seen in the elderly group (aged 70-79 years) (p=0.961 and p=0.007, respectively). By contrast, no significant difference in either CSS or OS was found in the younger group (p=0.550 and p=0.408, respectively). Intrinsic deaths were more likely to occur in elderly patients treated with radiotherapy than in those undergoing surgery (69.3% vs 78.2%, p=0.128).Conclusions. Our data suggests that surgery provided significantly better OS than radiotherapy, particularly among the elderly. However, radiotherapy may be more appropriate in elderly patients due to less invasiveness of the procedure. Prospective trials evaluating these therapies are warranted.
Not Residual Kidney Removal but Peritoneal Clearance Dominates in Uric Acid Control in Peritoneal Dialysis
Authors: Xiao Xi , Ye Hongjian , Yi Chunyan , Lin Jianxiong , Peng Yuan , Huang Xuan , Wu Meiju , Wu Haishan , Mao Haiping , Yu Xueqing , Yang Xiao 
Published: December 2019

Abstract
Background: There’s a paucity of systematic study focusing on clearance of uric acid (UA) in peritoneal dialysis (PD). The aim of this study was to investigate peritoneal UA removal and its influencing factors in PD patients. Methods: This was a cross-sectional study. Patients who performed peritoneal equilibration test (PET) and Kt/V from April 1, 2018 to August 31, 2019 were enrolled. The demographic data, clinical and laboratory parameters including the UA levels in the dialysate, blood and urine samples were collected. Results: Finally, there were 180 prevalent PD patients (52.8% male). Compared with normal serum UA (SUA) group, the hyperuricemia group showed significantly lower peritoneal UA clearance (39.1±6.2 vs. 42.0±8.0 L/week/1.73m2, P=0.008). Peritoneal UA clearance but not residual kidney removal was revealed to be independently associated with continuous [standardized coefficients (β) -0.21, 95% confidence interval (CI) -0.07, -0.006] and categorical SUA level [odds ratio (OR) 0.91, 95% CI 0.84,0.98]. Furthermore, the higher (high or high-average) transporters showed greater peritoneal UA clearance than the lower (low or low-average) transporters (42.0±7.0 vs. 36.4±5.6 L/week/1.73 m2, P<0.001). Among the widely used solute removal indicators, peritoneal creatinine clearance (CCL) performed best to predict higher peritoneal UA clearance in ROC analysis [area under curve (AUC) 0.96, 95% CI 0.93-0.99]. In multiple linear regression, lower albumin level (β -0.06, 95%CI -2.09, -0.19), higher transporters (β 0.06, 95%CI 0.05, 1.69) and greater peritoneal CCL (β 0.95, 95%CI 0.81, 0.93) were independently associated with higher peritoneal UA clearance. Conclusions: It was peritoneal removal but not residual kidney function that dominated in SUA balance of PD patients. Albumin level, peritoneal transport type and peritoneal CCL were identified as independent determinants of peritoneal UA clearance.
Assessing the measurement invariance of the CESD-10 and Beck anxiety inventory (BAI) questionnaires across people living with HIV/AIDS and healthy people
Authors: Bagheri Zahra , Noorshargh Pegah , Shahsavar Zahra , Jafari Peyman 
Published: December 2019

Abstract
Background Recently, extensive research has been reported the higher rate of depression and anxiety among people living with HIV/AIDS (PLWHAs) as compared to general population. However, no single study has been carried out to investigate whether this disparity is a real difference or it happens due to lack of measurement invariance. This study aims to assess the measurement invariance of the Beck Anxiety Inventory (BAI) and 10-item Centre for Epidemiological Studies Depression Scale (CESD-10) questionnaires across PLWHAs and healthy individuals.Methods One hundred and fifty PLWHAs and 500 healthy individuals filled out the Persian version of the BAI and CES-D10 questionnaires. Multi-group multiple-indicators multiple-causes model (MG-MIMIC) was used to assess measurement invariance across PLWHAs and healthy people.Results Our findings revealed that PLWHAs and healthy individuals perceived the meaning of all the items in the BAI and CESD-10 questionnaires similarly. In addition, although depression scores were significantly higher in PLWHAs as opposed to healthy individuals, no significant difference was observed in anxiety scores of these two groups.Conclusions The current study suggests that the BAI and CESD-10 are invariant measures across PLWHAs and CESD-10 which can be used for meaningful cross-group comparison. Therefore, in comparison to healthy individuals, higher depression scores of PLWHAs is a real difference. It is highly recommended that health professionals develop therapeutic interventions and psychological supports to promote the mental health of PLWHAs which alleviate their depressive symptoms.
A mental health assessment method based on emotion level derived from voice
Authors: Shinohara Shuji , Nakamura Mitsuteru , Omiya Yasuhiro , Hagiwara Naoki , Mitsuyoshi Shunji , Toda Hiroyuki , Saito Taku , Tanichi Masaaki , Yoshino Aihide , Tokuno Shinich 
Published: December 2019

Abstract
Background: In many developed countries, mental health disorders have become a problem, and the economic loss due to treatment costs and interference with work is immeasurable. Therefore, a simple technique must be developed to determine individuals’ depressive state and stress levels. Voice analysis using smartphones is not only noninvasive, it does not require a dedicated device; thus, it can be performed conveniently and remotely. Consequently, we developed a method to assess individuals’ mental health levels using emotional components contained in the human voice. Methods: We proposed two indices of mental health: a short-term index (vitality) and mental activity calculated from long-term trends in vitality. We used the voices of healthy individuals (men: n = 10, Mage = 42.7 ± 6.0 years; women: n = 4, Mage = 35.0 ± 14.4 years) and patients with major depression (men: n = 19, Mage = 43.7 ± 11.0 years; women: n = 11, Mage = 53.9 ± 8.2 years). For patients, simultaneously with voice collection, specialists assessed current depression severity using the Hamilton Rating Scale for Depression (HAM-D). Results: A significant negative correlation existed between the vitality extracted from voice and HAM-D score (r = -0.33, p < .05). We could discriminate the voice data of healthy individuals and patients with depression (judged as moderate or severe by the specialists) with high accuracy using vitality (p = .0085, the area under the curve (AUC) of the receiver operating characteristic curve = 0.87). However, there was no significant difference between the vitality of the healthy individuals and the patients judged to be the “no depression group with almost no depressive symptoms,” even if they were outpatients with depression (p > .1, AUC = 0.64). Conclusions: We developed a method to estimate stress through emotion instead of analyzing stress directly from voice data. By daily monitoring of vitality using smartphones, we can encourage hospital visits for people before they become depressed or during the early stages of depression. This may lead to reduced economic loss due to treatment costs and interference with work.
‘’They took us to the community to die from there’’: Patients’ and HIV service managers’ experiences of Differentiated ART delivery following national scale-up in Uganda
Authors: Zakumumpa Henry , Rujumba Joseph , Kwiringira Japheth , Katureebe Cordelia , Spicer Neil 
Published: December 2019

Abstract
Background Although Differentiated Service Delivery (DSD) for anti-retroviral therapy (ART) has been rolled-out nationally in several countries since World Health Organization (WHO)’s landmark 2016 guidelines, there is little research evaluating post-implementation outcomes. The objective of this study was to explore patients’ and HIV service managers’ perceptions of the early implementation experiences of national DSD roll-out across Uganda. Methods We utilized a qualitative research design involving 124 participants. Between April and June 2019 we conducted 76 semi-structured interviews with national-level HIV program managers (n=18), District Health Team leaders (n=24), representatives of PEPFAR implementing organizations (11), ART clinic in-charges (23) in six purposively selected Uganda districts with a high HIV burden (Kampala, Luwero, Wakiso, Mbale, Budadiri, Bulambuli). Six focus group discussions (48 participants) were held with patients enrolled in DSD models in case-study districts. Data were analyzed by thematic approach as guided by the multi-level analytical framework by Levesque et al. (2013): Individual-level factors; Health-system factors; Community factors; and Context. Results Our data shows that multiple barriers have been encountered in DSD implementation. Individual-level factors: Individualized stigma and a fear of detachment from health facilities by stable patients enrolled in community-based models were reported as bottlenecks. Socio-economic status was reported to have an influence on patient selection of DSD models. Health-system factors: Insufficient training of health workers in DSD delivery and supply chain barriers to multi-month ART dispensing were identified as constraints. Patients perceived current selection of DSD models to be provider-intensive and not sufficiently patient-centred. Community factors: Community-level stigma and insufficient funding to providers to fully operationalize community drug pick-up points were identified as limitations. Contextual factors: Frequent changes in physical addresses among urban clients were reported to impede the running of patient groups of rotating drug pick-ups. Conclusion This is one of the first multi-stakeholder evaluations of national DSD implementation in Uganda since initial roll-out in 2017. Multi-level interventions are needed to accelerate further DSD implementation in Uganda from demand-side (addressing self-stigma barriers, community engagement) and supply-side dimensions (strengthening ART supply chain capacities, increasing funding for community models and further DSD program design to improve patient-centeredness).
The effect of the employment of consultants in the Emergency Department on quality of care and equity – a quasi-experimental retrospective cohort study
Authors: Lindstroem Mette Bendtz , Andersen Ove , Kallemose Thomas , Rasmussen Line Jee Hartmann , Rosthoej Susanne , Jervelund Signe Smith 
Published: December 2019

Abstract
Background Crowding and bed occupancy are challenging issues in the hospitals with increasing acute admissions, caused by an aging population. Crowding in Emergency Departments (EDs) has a negative impact on length of hospitalisation, in-hospital mortality, patient safety, and flow. Thus, the Danish Health Authorities recommend the presence of specialist doctors in the ED who are dedicated to execute the clinical decision-making process. Thus, in 2016, the model of acute care was changed in the ED at Hvidovre Hospital, Denmark, to include consultant-led triage and continuous presence of consultants, referred to as Acute Medical Consultants. However, there is little evidence concerning the effect of consultants treating patients in the ED, and how it affects care for patients of varying socioeconomic status compared with other models of ED staffing. This study investigated whether the employment of Acute Medical Consultants in a Danish ED affected the quality of care for acutely admitted medical patients in terms of length of stay, readmission, mortality, and secondly how this effect was distributed across socioeconomic status in patients. Methods Admission data for 9,869 adult medical patients admitted for up to 48 hours in the ED was collected in two separate 7-month periods, one prior to and one after the organisational intervention. Linear regression and Cox proportional hazards regression analyses adjusted for age, sex, comorbidities, level of education, and employment status were applied. Results Following the employment of Acute Medical Consultants, an overall 11% increase in index-admissions was observed, and 90% of patients were discharged by an Acute Medical Consultant with a reduced mean length of stay by 1.4 hours (95% CI: 1.0 – 1.9). No significant change was found in in-hospital mortality, readmission, or mortality within 90 days after discharge. No difference was found in quality of care across socioeconomic status. Conclusion The employment of Acute Medical Consultants in the ED was associated with reduced length of admission without a negative effect on the quality of care for ED-admitted medical patients in general, or for patients with lower socioeconomic status. Yet, in order to reduce readmission and mortality among acutely admitted patients, other means must be initiated.
ANALISIS KANDUNGAN ZAT GIZI MAKRO DAN DAYA CERNA PATI SNACK BAR TUJOGUNG (TEPUNG UBI JALAR ORANYE DAN JAGUNG) SEBAGAI ALTERNATIF MAKANAN SELINGAN PENDERITA DIABETES TIPE 2
Authors: Rahmawati Ika 
Published: December 2019

Diabetes melitus merupakan kumpulan gejala yang timbul pada seseorang yang mengalami peningkatan kadar glukosa darah. Kadar glukosa darah dapat dikendalikan melalui konsumsi makanan dengan tingkat indeks glikemik yang rendah serta makanan dengan daya cerna pati lambat cerna. Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan tepung ubi jalar oranye dan jagung pada pembuatan snack bar terhadap zat gizi makro dan daya cerna pati untuk penderita diabetes tipe 2. Penelitian ini menggunakan rancangan percobaan acak lengkap (RAL) satu variabel. Penggunaan tepung ubi jalar oranye dan jagung dilakukan dengan tiga taraf (25:75, 30:70, dan 35:65). Hasil uji Kruskal-Wallis taraf penggunaan tepung ubi jalar oranye dan jagung memberikan pengaruh terhadap mutu hedonik dan tingkat kesukaan (hedonik) snack bar (p<0,05). Formula terbaik adalah snack bar dengan formula 1 (25:75) dengan nilai rata-rata hedonik dan mutu hedonik tertinggi yaitu berturut-turut 3,4 dan 3,7. Hasil analisis kimia Snack bar terpilih yaitu kadar air 53,4%, kadar abu 2,1%, kadar protein 9,3%, kadar lemak 8%, dan kadar karbohidrat 27,3%. Snack bar terpilih mengandung daya cerna pati sebesar 22,5% yang termasuk ke dalam kategori pati lambat cerna.
Kata kunci: Diabetes Tipe 2, Daya Cerna Pati, Snack Bar, Ubi Jalar Oranye, Jagung
ASUPAN KARBOHIDRAT DAN PROTEIN BERHUBUNGAN DENGAN STATUS GIZI ANAK SEKOLAH DI SYAFANA ISLAMIC SCHOOL PRIMARY, TANGERANG SELATAN TAHUN 2017
Authors: Parinduri Mutia Sari , Safitri Debby Endayani 
Published: December 2019

Status gizi pada anak sekolah dipengaruhi oleh jumlah asupan pangan yang dikonsumsi. Terdapat faktor yang memengaruhi status gizi yaitu faktor langsung (asupan makanan dan penyakit infeksi) dan faktor tidak langsung (ketahanan pangan keluarga, pola pengasuhan anak, lingkungan kesehatan dan akses pelayanan kesehatan). Penelitian ini bertujuan untuk mengetahui hubungan konsumsi fast food, minuman berkalori, asupan makanan dengan status gizi anak sekolah di Syafana Islamic School yang dilaksanakan pada bulan Oktober-November 2017. Penelitian ini menggunakan penelitian deskriptif dengan desain cross-sectional. Sampel berjumlah 175 orang. Pengambilan sampel dilakukan dengan metode propotional random sampling. Pengambilan data dengan observasi dan wawancara langsung menggunakan kuesioner, FFQ dan recall 2 x 24 jam. Data yang diambil berupa karakteristik siswa (jenis kelamin dan uang saku), konsumsi fast food dan minuman berkalori, asupan makanan (energi dan zat gizi makro), dan status gizi serta menggunakan uji statistik chi-square. Hasil uji statistik chi-square menunjukkan terdapat hubungan antara asupan karbohidrat (p<0,05), dan asupan protein (p<0,05) dengan status gizi. Tidak terdapat hubungan antara konsumsi fast food dan minuman berkalori (p>0,05), asupan energi (p>0,05) dan asupan lemak (p>0,05) dengan status gizi.

Kata Kunci: Anak Sekolah, Status Gizi, Asupan Makanan
Penggunaan Tepung Daun Kelor pada Pembuatan Crackers Sumber Kalsium
Authors: Mazidah Yustika Fahreina , Kusumaningrum Indah , Safitri Debby Endayani 
Published: December 2019

Moringa oleifera (kelor) merupakan salah satu sayuran hijau yang kaya akan zat gizi dan mulai dikembangkan dalam produk pangan olahan. Tujuan penelitian ini adalah untuk memanfaatkan daun kelor untuk dijadikan crakers sebagai selingan yang disukai remaja dan dapat memenuhi kebutuhan gizi remaja. Penelitian ini menggunakan rancangan acak lengkap dengan 2 kali pengulangan. Substitusi tepung daun kelor terhadap tepung terigu pada pembuatan crackers yaitu, F0 (100:0), F1 (90:10), F2 (85:15), dan F3(80:20). Penentuan crackers daun kelor terbaik menggunakan uji hedonik dengan panelis semi terlatih. Analisis data menggunakan uji Kruskall Wallis, dengan uji lanjut Mann Whitney. Semakin tinggi tingkat substitusi tepung daun kelor terhadap tepung terigu, tingkat kesukaan panelis terhadap warna, tekstur, rasa, dan aroma crackers semakin menurun. Produk crackers terpilih adalah F1, dengan warna hijau kecoklatan, tekstur renyah, rasa tidak pahit, dan aroma biasa. Produk crackers terpilih dalam 100 g mengandung energi 456,1 kkal, karbohidrat 67,1 g, protein 8,9 g, lemak 16,9 g, zat besi 3,05 mg, dan kalsium 324 mg. Crackers daun kelor terpilih dapat diklaim sebagai makanan sumber kalsium.
Kata kunci: Moringa oleifera, Tepung daun kelor, Crackers daun kelor, Kalsium, Zat besi
Telenovela e Recepção: Um estudo sobre o público masculino universitário
Authors: Carvalho Alessandra Pinto de , Santos Andreza Patricia Almeida dos 
Published: December 2019

Espaço privilegiado para se pensar a cultura brasileira, a telenovela se destaca como fonte inesgotável de apropriações. Cientes da importância de se entender os usos feitos por diversos segmentos sociais, geracionais e regionais, este ensaio explora – a partir de uma abordagem etnográfica baseada na análise de Gluckman e da aplicação de questionário de hábitos televisivos e consumo de telenovela – um pouco da relação estabelecida entre a telenovela e o público masculino universitário. Analisamos como, em um universo acadêmico, se constroem formas de interação e de amizade a partir desse formato televisivo.
EFECT OF LEAF FERTILIZER ON SECOND TREATMENT TO THREE GENOTYPES CORN EFFICIENT CROPS IN TIDAL LAND
Authors: Andesta Andesta , Munandar Munandar , Yakup Yakup 
Published: December 2019

This study aims to determine the effect of leaf fertilizer on three nutrient efficient corn genotypes in tidal land. This research was coducted in Village Mulia sari, Sub-district Tanjung Lago, District Banyuasin, South Sumatra in the noble village of Sari Tanjung Lago sub-district from August to December 2017. This study uses the Split Plot Design consisting of 3 factors, namely the first factor of genotype, the second factor is the dose of urea A0 = 0 kg, A1 = 250 kg, A2 = 300 kg and the third factor of leaf fertilizer consists of: P0 = (control without spraying), P1 = 30 days of spray, P2 = 45 days of spray, P3 = 30 + 45 days of spray, P4 = 60 days of spray, P5 = 75 days of spray, P6 = 60 + 75 days of spray. repeated three times. Observation results used of 300 kg of urea fertilizer and leaf fertilizer had a good influence on the growth of G3 corn genotypes and the production of corn plants in tidal land. The combination of giving 300 kg of urea fertilizer + leaf fertilizer gives a pretty good influence on the growth of G3 corn genotypes with a production of 7.44 Ton/hectares