bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of human papillomavirus genotypes and precancerous cervical lesions in a screening population in Beijing, China: Analysis of results from China’s Top 3 Hospital, 2009–2019
Authors: Liu Yidi , Wu Huan , Xu Jingjiang , Chen Defu , Zhao Hongyou , Liu Haolin , Guo Xianghuan , Qiu Haixia , Gu Ying 
Published: March 2020

Abstract
Background: Cervical cancer is the fourth most common cancer in women. Early detection and diagnosis play an important role in secondary prevention of cervical cancer. This study aims to provide more information to develop an effective strategy for the prevention and control of cervical cancer in northern China. Methods: A retrospective single-centre descriptive cross-sectional study was conducted in Chinese PLA General Hospital located in Beijing, covering the period from January 2009 to June 2019. The patients who underwent a polymerase chain reaction (PCR)-based HPV genotyping test and cervical pathological diagnosis were included. Furthermore, we limited the interval between the two examination within 180 days for the purpose of making sure their correlation to analyse their relationship. Moreover, the relationship between different cervical lesions and age as well as single/multiple HPV infection was assessed.Results: A total of 3,134 patients were eligible in this study after HPV genotyping test and pathological diagnosis. Most of the patients (95%) were from northern China. Among the patients, 1,745(55.68%） had high-grade squamous intraepithelial neoplasia (HSIL), 1,354 (43.20%) had low-grade squamous intraepithelial neoplasia (LSIL) and 35 (1.12%) were Normal. The mean age was 42.06 ± 10.82(range, 17–79 years). The women aged 35-49 years accounted for the highest incidence rate. The top five most commonly identified HPV genotypes in each lesion class were as follows: HPV16, 58, 52, 31 and 51 in the class of HSIL; HPV16, 52, 58, 56 and 51 in the class of LSIL; HPV16, 31, 6,11, 52 and 58 in the class of normal. The frequencies of HPV single genotype infection and multiple genotypes infection were 55.26% and 34.18%, respectively. There was no difference in the attributable proportions of multiple genotypes infection amongst HSIL, LSIL and Normal.Conclusions: In Northern China, HPV16 was the most dominant genotype in the patients with pathological examination. The peak age of the onset of HSIL was between 35 and 49 years of age. Infection with multiple HPV genotypes did not increase the risk of HSIL. Type-specific HPV prevalence and attribution proportion to cervical precancerous lesions should be taken into consideration in the development of vaccines and strategy for screening in this population.
Effect of Streptococcus anginosus on biological response of tongue squamous cell carcinoma cells
Authors: Xu Yuan , Jia Yuhuan , Chen Liang , Gao Jing , Yang Deqin 
Published: March 2020

Abstract
Background: Streptococcus anginosus(S.anginosus) was reported increased in oral squamous cell carcinoma(OSCC) tissue. The aim of this study was to investigate the response of oral cancer cells in the biological characteristics evoked by the S.anginosus and investigate its potential mechanisms.Methods: The growth curve and concentration standard curve of S.anginosus were determined , and a series of concentrations of S.anginosus supernatant were applied to OSCC cell lines SCC15,then selected an optimal time and concentration by CCK-8 assay. Then autophagic response, proliferative activity, cell cycle and apoptosis, invasion and migration abilities were evaluated in SCC15.Results: The results showed that when the ratio of S.anginosus supernatant to cell culture medium was 1:1 and the co-culture time was 16h, there was a most significant inhibited effect on SCC15; Furthermore, S.anginosus supernatant can up-regulate the autophagy activity of SCC15, then, the ability of proliferation, migration and invasion was observed inhibited obviously. Compared with the control groups, the cell cycle showed G1 arrest, S and G2 / M phases decreased, and the percentage of apoptotic cells relatively increased(P<0.05).Conclusion: S.anginosus can reduce the proliferation, migration and invasion of SCC15 cells and promote cell apoptosis; Moreover, autophagy may be one of the mechanisms in this process.
Hepatitis C Virus nonstructural protein 4B induces lipogenesis via Merlin-Hippo pathway
Authors: Zhang Zhanfeng , Zhao Rong , Chen Jiukai , Xie Zaichun , Pang Zhiyu , Zhou Yingchun 
Published: March 2020

Abstract
Hepatitis C virus (HCV) infection is often associated with hepatic steatosis, even hepatocellcular carcinoma. Nonstructural protein 4B (NS4B)，a highly hydrophobic non-structural protein, induces lipogenesis, but the underlying mechanism remains incompletely understood. In the present study, NS4B expression in Huh7.5 cells could reduce moesin-ezrin-radixin–like protein (Merlin, NF2 gene coding protein) expression, Yap phosphorylation, and increase AKT phosphorylation and sterol regulatory element-binding proteins (SREBPs) expression, which regulate lipogenic gene expressions including fatty acid synthase (FAS). In Huh7.5 cells, Merlin silencing reduced Yap phosphorylation, AKT phosphorylation, and especially SREBPs levels lead to reduction of lipid drop synthesis. In addition, HCV NS4B infected Huh7.5 cells exhibited lipid droplets, but lipid droplets significantly reduced after Merlin over-expressed. In conclusion, these results indicated that NS4B may play an important role in HCV-associated liver pathogenesis via Merlin-Hippo signal pathway.
A modified surgical technique of shortening renal ischemia time in left renal cancer patients with Mayo level II-IV tumor thrombus
Authors: Ma Lulin , Zhuo Liu , Guodong Zhu , Xun Zhao , Shiying Tang , Peng Hong , Li Zhang , Liwei Li , Shudong Zhang , Guoliang Wang , Xiaojun Tian , Cheng Liu , Hongxian Zhang 
Published: March 2020

Abstract
Objective to explore the safety and effectiveness of a modified surgical technique which could shorten the time of renal ischemia in left renal cancer and Mayo level II to IV inferior vena cava (IVC) tumor thrombusMethod We retrospectively analyzed the clinical data of 14 cases with left renal cell carcinoma (RCC) and Mayo level II to IV IVC tumor thrombus. Preoperative imaging showed that there was no obvious sign of tumor thrombus invading the blood vessel wall. During the surgery, after the right renal artery, the right renal vein and the distal end of IVC were blocked, the balloon catheter was used and the tumor thrombus was removed completely from the IVC. The incision of IVC was closed by Satinsky clamp to make IVC partially blocked. Then the right renal artery and right renal vein were released. The incision of IVC was sutured continuously. At last, the Satinsky clamp and the blocking band at the distal end of the IVC were released.Result There were 8 cases (57.1%) of Mayo level II, 3 cases (21.4%) of Mayo level III and 3 cases (21.4%) of Mayo level IV. The operation was successfully completed in all 14 patients. There were 2 cases (14.3%) operated by complete laparoscopic approach, 8 cases (57.1%) by open approach, and 4 patients (28.6%) by laparoscopic conversion to open approach. The occlusion time of right renal artery and vein (renal ischemia time) was 3 to 15 minutes, with an average of (6.8±3.2) minutes. The mean time of IVC occlusion was (19.4±4.9) min. Preoperative creatinine was 66 to 130 μmol/L, with an average of (96.6 ± 21.2) μmol/L. One week after operation, serum creatinine was 64 to 632 μmol/L, with an average of (132.4 ± 144.9) μmol/L. Among the 14 cases, 5 (42.9%) had early postoperative complications. Besides one of the 14 patients died in perioperative period, the median follow-up of other 13 cases was 10 months (range: 4-29 months). The 5 (35.7%) of the 14 cases were died of disease.Conclusion This modified procedure was relatively safe and effective in shortening the time of renal ischemia in left RCC patients with Mayo II to IV IVC tumor thrombus.
Onabotulinumtoxin A improves psychological aspects in chronic migraine patients
Authors: Al-Hashel Jasem Yousef , Ashkanani Hasan Kh , Almutairi Ohood , Bokubar Fajer A , Mubarak Shahad , Alwazzan Sawsan , Alroughani Raed , Youssry Doaa , Ahmed Samar Farouk 
Published: March 2020

Abstract
Background: Chronic migraine (CM) affects 5.4% of the Kuwaiti population. It is associated with significant headache-related disability, psychiatric comorbidity and reduces quality of life. The aim of this study is to assess the efficacy of OnabotulinumtoxinA on psychological aspects of chronic migraine patients.Methods: 113 chronic migraineurs were identified in a tertiary headache center. Eligible patients met International Classification of Headache Disorders disorders-third edition, beta version (ICHD-III)Revision criteria for chronic migraine. They received 155 units of Onabotulinumtoxin A quarterly according to the Phase III Research Evaluating Migraine Prophylaxis Therapy Trail (PREEMPT) protocol. Patients received less than 4 injections cycles were excluded. Quality of life, the seven-item Generalized Anxiety Disorder (GAD-7) scores, the nine-item Patient Health Questionnaire (PHQ9), The Pittsburgh Sleep Quality Index (PSQI) were collected before injection and at last visit. Mean comparison tests were performed using the independent sample t test to assess the effects of onabotulinumtoxin A on quality of life, comorbid symptoms of anxiety, depression, and quality of sleep. Results: This is retrospective study included 113 chronic migraine patients, with a mean age of 44.92+ 9.47years, mean disease duration 12.20 +10.10 years and a mean treatment duration 33.72 +43.14 months. At last visit, most of our cohort showed improvement in quality of life (81%), GAD-7 (81%), PHQ9 (79%), and PSQ1 (76%). The mean score of patient satisfaction was 7.21 +2.21. OnabotulinumtoxinA treatment for CM improved Quality of life significantly (72.92 +17.34 versus103.62 +15.62;P< 0.0001). It was also associated with significant reduction in GAD-7 (12.00 +4.40 versus 6.61 +4.40; P< 0.0001); PHQ-9 (17.91 +8.43 versus12.52 +8.77; P< 0.0001) scores and PSQI (12.60 +5.66 versus 6.66 +5.04; P< 0.0001) at last visit.Conclusion: Prophylactic OnabotulinumtoxinA treatment for CM was associated with significant improvement of quality of life, reduction in symptoms of anxiety and depression, and improved associated symptoms of poor sleep.
Fall Characteristics Among Elderly Populations in Urban and Rural Areas in Korea
Authors: Kim Myeongkyu , Chang Misoo , Nam Eunwoo , Kim Seul Gi , Cho Sung-il , Ryu Dong Hee , Kam Sin , Choi Bo Youl , Kim Mi Jung 
Published: March 2020

Abstract
BackgroundFalling is one of the leading causes of injury among elderly populations. As the population over 65 years old increases, medical costs due to falling will also increase. Urban and rural areas have different fall characteristics, and research into these differences is lacking.MethodsA survey was conducted on 2,012 people over 60 years old between September 1, 2015 to October 12, 2015. Guro-gu (Seoul), Yeongdeungpo-gu (Seoul), and Jung-gu (Daegu) were classified as urban areas and included 1,205 of the study participants. Dalseong-gun (Daegu) and Yangpyeong-gun (Gyeonggi-do) were categorized as rural areas and included 807 participants. Several region characteristics, including population density, were considered to distinguish between urban and rural areas. The survey included questions about fall history, cause, season and time of recent falls, and external conditions associated with recent falls, like floor or ground material and shoe types.ResultsRural respondents were older than urban respondents (p<0.001) but did not differ significantly in gender proportion (p=0.082). Fall history over the past year was not different between the two regions (p=0.693), but lifetime fall history was greater among rural respondents (p<0.001). Only 5.1% of all respondents had undergone fall-prevention education. A slippery floor was the most common cause of falls in both regions, but there was a significant difference in pattern of fall causes (p<0.001). Falls were more frequent in the summer, spring, and the afternoon in urban areas, and in the summer, autumn, and the morning in rural areas. Cement and asphalt were the most common ground materials at the time of falls in both regions, but rural respondents had higher fall rates when walking on soil and when wearing slippers.ConclusionsA fall-prevention program that reflects the characteristics and differences of falls in urban and rural areas should be developed and used to effectively prevent falling among elderly people.
Clinical efficacy of extended modified posteromedial approach versus posterolateral approach for surgical treatment of posterior pilon fracture: a retrospective analysis
Authors: Zhang Qin-Ming , Wang Hai-Bin , Li Xiao-Yan , Chu Feng-Long , Han Liang , Li Dong-Mei , Wu Bin 
Published: March 2020

Abstract
Background: Posterior pilon fracture is a type of ankle fracture associated with poorer treatment results compared to the conventional ankle fracture. This is partly related to the lack of consensus on the classification, approach selection, and internal fixation method for this type of fracture. This study aimed to investigate the clinical efficacy of posterolateral approach versus extended modified posteromedial approach for surgical treatment of posterior pilon fracture. Methods: Data of 67 patients with posterior pilon fracture who received fixation with a buttress plate between January 2015 and December 2018 were retrospectively reviewed. Patients received steel plate fixation through either the posterolateral approach (n = 35, group A) or the extended modified posteromedial approach (n = 32, group B). Operation time, intraoperative blood loss, excellent and good rate of reduction, fracture healing time, American Orthopaedic Foot & Ankle Society (AOFAS) Ankle-Hindfoot Scale score, and Visual Analogue Scale score were compared between groups A and B. Results: All patients were followed up for an average period of 15.4 months. No nonunion, failure of internal fixation, or anklebone stiffness occurred in either group during the follow-up period. However, the operation time was significantly shorter, intraoperative blood loss was significantly lower, AOFAS Ankle-Hindfoot Scale score was significantly higher, and Visual Analogue Scale score was significantly lower in group B than in group A (P < 0.05). Conclusion: Compared to the posterolateral approach, the extended modified posteromedial approach can provide a better surgical field for the treatment of posterior pilon fracture, which allows reduction and fixation of this type of fractures under direct vision and evaluation of reduction effects, and reduces operation time and intraoperative blood loss. Combining this approach with supporting steel plate fixation enables early functional rehabilitation of the ankle with more satisfactory clinical results.
CircRHOT1 Mediated Cell Proliferation, Apoptosis and Invasion of Pancreatic Cancer Cells by Sponging miR-125a-3p
Authors: Ling Sunkai , He Yanru , Li Xiaoxue , Hu Mingyue , Ma Yu , Li Yuan , Lu Zipeng , Shen Shanshan , Kong Bo , Zou Xiaoping , Jiang Kuirong , Huang Peilin 
Published: March 2020

Abstract
Background: The present study aimed to investigate the mechanistic biological function of circRHOT1 in pancreatic cancer cells.Methods: The expression of circRHOT1 and miR-125a-3p in pancreatic cancer tissues and their paired adjacent normal tissues was quantified by qRT-PCR. By knocking down or overexpressing circRHOT1 and miR-125a-3p in pancreatic cancer cells, their functions and potential mechanisms were explored.Results: circRHOT1 was overexpressed in pancreatic cancer tissues and cell lines, and it was found to directly bind to miR-125a-3p, acting as an endogenous sponge to inhibit its activity. Knockdown of circRHOT1 expression significantly inhibited proliferation as well as invasion, and it promoted apoptosis of pancreatic cancer cells via the regulation of E2F3 through the targeting of miR-125a-3p.Conclusion: Taken together, our results demonstrated that circRHOT1 plays critical roles in regulating the biological functions of pancreatic cancer cells, suggesting that circRHOT1 may serve as a potential diagnostic marker and therapeutic target for patients with pancreatic cancer.
Prenatal Diagnosis of Fetal Skeletal Dysplasia Using 3-Dimensional Computed Tomography: A Case Series
Authors: Waratani Miyoko , Ito Fumitake , Tanaka Yukiko , Mabuchi Aki , Mori Taisuke , Kitawaki Jo 
Published: March 2020

Abstract
Background: Fetal skeletal dysplasia constitutes a complex group of systemic bone and cartilage disorders. Many fetal skeletal dysplasia phenotypes have indistinct definitions, making definitive prenatal diagnosis difficult. Fetal skeletal dysplasia is typically diagnosed using sonography; however, three-dimensional computed tomography (3D-CT) also aids in making a prenatal diagnosis. This study aimed to examine the efficacy of 3D-CT for the prenatal diagnosis of fetal skeletal dysplasia by comparing the diagnostic accuracy of fetal sonography and 3D-CT.Methods: Based on the suspicion of skeletal dysplasia based on ultrasound examination, we performed 3D-CT prenatally to confirm this diagnosis. In order to minimize exposure of the fetuses to radiation without compromising image quality, we used predetermined 3D-CT settings for volume acquisition.Results: Sixteen fetuses were suspected to have fetal skeletal dysplasia based on ultrasonography findings. Of these, 15 were diagnosed with fetal skeletal dysplasia using 3D-CT. All 15 fetuses diagnosed with fetal skeletal dysplasia prenatally were confirmed postnatally to have fetal skeletal dysplasia. The definitive postnatal diagnosis (campomelic dysplasia) differed from the prenatal diagnosis (osteogenesis imperfecta) in only one infant. 14 cases (93.3%) were diagnosed by an accyrate classification. Five infants had lethal skeletal dysplasia; one died in utero, and four died as neonates. We determined the appropriate delivery method for each infant based on the prenatal diagnosis.Conclusion: 3D-CT is a valuable tool to augment ultrasound examination for the diagnosis of skeletal dysplasia because it can provide a more definitive diagnosis of the type of dysplasia, enabling better planning for resuscitation of the infant after birth.Trial registration: University Hospital Medical Information Network (UMIN) Center trial registration number is UMIN000034744. Registered 1 October, 2018 – Retrospectively registered, https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=Roooo39610.
LncRNA PRNCR1 promotes breast cancer proliferation and inhibits apoptosis by modulating microRNA-377/CCND2/MEK/MAPK axis
Authors: Ouyang Jian , Liu Zilong , Yuan Xiaobing , Chen Xia , Wang Yongpeng , Liu Lu , Long Chunping , Liang Hui 
Published: March 2020

Abstract
Background: Long non-coding RNAs (lncRNAs) have recently become the vital gene regulators in diverse cancers. In our study, we purposed to inquiry into the mechanisms of lncRNA PRNCR1 in breast cancer via microRNA-377 (miR-377)/CCND2/MEK/MAPK axis.Methods: PRNCR1 expression in breast cancer tissues was detected, and the correlation between PRNCR1 expression and prognostic survival was analyzed. The expressions of PRNCR1 and miR-377 in breast cancer cell lines were detected. Relationships among PRNCR1, miR-377 and CCND2 were confirmed by luciferase activity, RNA pull-down or RIP assays. Breast cancer cells were introduced with silenced PRNCR1 or restored miR-377 to explore their functions in breast cancer’s malignant phenotype. Related proteins expression in MEK/MAPK pathway was determined by western blot analysis. Results: PRNCR1 was abnormally highly expressed and miR-377 was poorly expressed in breast cancer, and patients with high expression of PRNCR1 had a poor prognosis. PRNCR1 silencing or miR-377 overexpression resulted in suppressed breast cancer cell proliferation ability, blocked cell cycle process and induced apoptosis. PRNCR1 may participate in the modulation of CCND2 by competitively binding with miR-377. CCND2 activated the MEK/MAPK pathway, and after treatment with Mirdametinib, the MEK/MAPK pathway was inhibited, which was found to retard breast cancer growth.Conclusion: Our study highlights that lncRNA PRNCR1 may competitively bind to miR-377, leading to upregulated CCND2, which in turn leads to MEK/MAPK pathway activation, thereby promoting breast cancer growth.
Effectiveness of a family-centered behavioral and educational counselling approach to improve periodontal health of pregnant women: a randomized controlled trial
Authors: Liu Pei , Wen Weiye , Yu Ka Fung , Gao Xiaoli , Lo Edward Chin Man , Wong May Chun Mei 
Published: March 2020

Abstract
Objectives: Poor oral hygiene and higher hormone levels during pregnancy always lead to poor periodontal health. Oral health education of the pregnant women to improve the periodontal outcomes might be ineffective and inconsistent. The aim of this study was to assess the effectiveness of a family-centered behavioral and educational counselling program on the pregnant women’s periodontal health and long-term effect after delivery. Methods: A randomized controlled trial was conducted among first-time pregnant women (10~20th gestational-week) in Hong Kong. Participating families were randomized into test and control group. The intervention in test group included: explanation on oral health education pamphlets, oral hygiene practice training by the “tell-show-do” technique, individualized feedback, and proposed solutions to overcome barriers on self-care. Reinforcement was implemented by interview, phone call or messaging to assess performance, identify barriers and to encourage participants on self-care, in around 32nd gestational-week and 6 months after delivery. Main outcomes assessed were periodontal health measurement including bleeding on probing (BOP), periodontal-pocket (Poc) and loss of clinical attachment levels (LoA); oral hygiene status was assessed by visible plaque index (VPI). The dental examinations were carried out at baseline, 32nd gestational-week and 12-months post-delivery.Results: 589 pregnant women were recruited and 369 completed all three visits (test 188; control 181). Mixed-effect model ANOVA indicated that in test group, % sites with visible plaque decreased from the baseline to the 32nd gestational-week and maintained to 12-months post-delivery while in control group the effect could not be sustained (p<0.001). Both groups showed significant decrease in BOP over time, and significantly lower % sites with BOP was found in test group than in control group at 12-months post-delivery (p<0.001). Generalized linear mixed model (GLMM) showed that there was significant less in sites with Poc and LoA in the both groups over time at 12-months post-delivery than during pregnancy (p<0.001), whereas there was no significant effect between the two groups (p>0.05). Conclusions: Providing family-centered, behavioral and educational counselling to pregnant women at early stage of pregnancy and reinforcements can improve their oral hygiene and periodontal health, the effect can be sustained over a longer period.