bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soot removal from ancient Egyptian complex painted surfaces using a double network gel: Empirical tests on the ceiling of the sanctuary of Osiris in the temple of Seti I – Abydos
Authors: Al-Emam Ehab Awad , Motawea Abdel Ghafour , Caen Joost , Janssens Koen 
Published: July 2020

Abstract
In this study, we evaluated the ease of removal of soot layers from ancient wall paintings by employing double network gels as a controllable cleaning method. The ceiling of the temple of Seti I (Abydos, Egypt) is covered with thick layers of soot; this is especially the case in the sanctuary of Osiris. These layers may have been accumulated during the occupation of the temple by Christians, fleeing the Romans in the first centuries A.D.. Soot particulates are one of the most common deposits to be removed during conservation-restoration activities of (Egyptian) wall paintings. They usually mask the painted reliefs and reduce the permeability of the painted surface. A Polyvinyl alcohol-borax/agarose (PVA-B/AG) double network gel was selected for this task since its properties were expected to be compatible with the cleaning treatment requirements. The gel is characterized by its flexibility, permitting to take the shape of the reliefs, while also having self-healing properties, featuring shape stability and an appropriate capacity to retain liquid. The gel was loaded with several cleaning reagents that proved to be effective for soot removal. Two sets of soot removal tests were conducted with these gel composites. The cleaned surfaces were examined in situ with the naked eye and with a digital microscope in order to select the best gel composite. The gel composite, loaded with a solution of 5% ammonia, 0.3% ammonium carbonate, and 0.3% EDTA yielded the most satisfactory results and allowed to safely remove a crust of thick soot particles from the surface. Thus, during a third phase of the study, it was used successfully to clean a larger area of the ceiling.
Gene Expression Profiling For Diagnosis of Multiple Primary Malignant Tumors
Authors: Zheng Yu , Sun Yifeng , Kuai Yue , Fu Guoxiang , An Huimin , Chen Jinying , Zhu Jiajun , Wu Yiwang , Song Kaibin , Xu Qinghua , Wu Di , Huang Deshuang , Pan Hongming 
Published: July 2020

Abstract
Background: The incidence of multiple primary malignant tumors (MPMTs) is rising due to the development of screening technologies, significant treatment advances and the increased aging of the population. For patients with a prior cancer history, distinguishing tumor recurrence or metastasis from a second malignant tumor has important prognostic and therapeutic implications and still represents a difficult problem in clinical practice. Methods: In this study, we evaluated the performance of a 90-gene expression assay and explored its potential diagnostic utility for MPMTs across a broad spectrum of tumor types. Twenty-four MPMT patients from Sir Run Run Shaw Hospital, college of medicine, Zhejiang University were enrolled in this study. A total of 51 MPMT specimens met all quality control criteria and were analyzed by the 90-gene expression assay. Results: For each clinical specimen, the tumor type predicted by the 90-gene expression assay was compared with its reference diagnosis with an overall accuracy of 94.1% (48 of 51, 95% confidence interval: 0.83-0.98). Additionally, the hierarchical clustering of 90-gene expression profiling in 51 specimens revealed MPMT samples were grouped together depending on tumor types or system types rather than individual MPMT patients. Conclusions: Therefore, the 90-gene expression assay provides flexibility and accuracy in identifying the tissue origin of MPMTs, especially in squamous cell carcinoma. Future incorporation of the 90-gene expression assay in the pathological diagnosis will assist oncologists in applying precise treatments, leading to improved care and outcomes for MPMT patients.
Association of Spectroscopically Determined Leaf Nutrition Related Traits and Breeding Selection in Sassafras Tzumu
Authors: Li Yanjie , Liu Wenjian , Tan Zifeng , Jiang Jingmin , Liu Jun 
Published: July 2020

Abstract
Background: The nutrition related to traits is an influential role in tree growth, tree production and nutrient cycling. Therefore, the influence of genetic parameters on leaf nutrition traits ought to take account of optimal tree breeding selection. However, the measurement methods are seriously affected by the progress of breeding selection program. In this study, we tested the ability of spectroscopy to quantify the specific leaf nutrition traits including Anthocyanins (ANTH), flavonoids (FLAV) and Nitrogen balance index (NBI), and estimated the genetic variation of these leaf traits based on the spectroscopic predicted data. Live fresh leaves of Sassafras tzumu were selected for spectral collection, after which concentrations of ANTH, FLAV and NBI were analyzed by standard analytical methods. Partial least squares regression (PLSR), five spectra pre-processing methods, and four variable selection algorisms were conducted for the optimal prediction model selection. Each trait model was simulated 200 times for error estimation.Results: The stander normal variation (SNV) to the ANTH model and 1st derivatives to the FLAV and NBI models, combined with significant Multivariate Correlation (sMC) algorithm variable selection are finally regarded as the best performance model. The ANTH model produced the highest accuracy of prediction with a mean R2 of 0.72 and mean RMSE of 0.10 %, followed by FLAV and NBI model (mean R2 =0.58, mean RMSE = 0.11 % and mean R2 =0.44, mean RMSE = 0.04 %). High heritability was found of ANTH FLAV and NBI with h2 of 0.78, 0.58 and 0.61 respectively. It shows that it is benefitting and possible of breeding selection for the improvement of leaf nutrition traits.Conclusions: Spectroscopy can successfully characterize the leaf nutrition traits in living tree leaves and the ability to simultaneous multiple plant traits provides a promising and high-throughput tool for the quick analysis of large size samples and serves for genetic breeding program.
Autosomal recessive polycystic kidney disease: case report of a newborn with rare PKHD1 mutation, rapid renal enlargement and early fatal outcome
Authors: Serra Gregorio , Corsello Giovanni , Antona Vincenzo , D'Alessandro Maria Michela , Cassata Nicola , Cimador Marcello , Giuffrè Mario , Schierz Ingrid Anne Mandy , Piro Ettore 
Published: July 2020

Abstract
Introduction Autosomal recessive polycystic kidney disease (ARPKD; MIM#263200) is one of the most frequent pediatric renal cystic diseases, with an incidence of 1:20,000. It is caused by mutations of the PKHD1 gene, on chromosome 6p12. The clinical spectrum is highly variable, ranging from late-onset milder forms to severe perinatal manifestations. The management of newborns with severe pulmonary insufficiency is challenging, and causes of early death are sepsis or respiratory failure. In cases of massive renal enlargement, early bilateral nephrectomy and peritoneal dialysis may reduce infant mortality. However, there is no conclusive data on the role of surgery, and decision-making is driven by patient’s clinical condition and expertise of the center. Patient presentation We hereby describe a preterm female newborn with perinatal, rapid and bilateral, abnormal growth of both kidneys, respiratory failure and initial signs of liver disease. She was subsequently confirmed to be affected by a rare and severe homozygous mutation of the PKHD1 gene, inherited from both her consanguineous parents. Our patient died 78 days after birth, due to a fungal sepsis which worsened her respiratory insufficiency. Conclusions This patient report shows some of the clinical and ethical issues of neonatal ARPKD, and the need of multidisciplinary approach and good communication with the family. Target next generation sequencing (NGS) techniques may guide and support clinicians, as well as guarantee to these patients the most appropriate clinical management, avoiding unnecessary and/or disproportionate treatments.
Novel botanical therapeutic NB-02 effectively treats Alzheimer’s neuropathophysiology in an APP/PS1 mouse model
Authors: Lee Yee Fun , Lariviere Amanda , Russ Alyssa N , Choi Sang-Zin , Bacskai Brian , Kastanenka Ksenia 
Published: July 2020

Abstract
Background Alzheimer’s disease (AD) is an incurable neurodegenerative disorder and a major cause of dementia. Some of the hallmarks of AD include presence of amyloid plaques in brain parenchyma, calcium dysregulation within individual neurons, and neuroinflammation. A promising therapeutic would reverse or stymie these pathophysiologies in an animal model of AD. Methods We tested the effect of NB-02, previously known as DA-9803, a novel multimodal therap­eutic, on amyloid deposition, neuronal calcium regulation and neuroinflammation in 8-10 month old APP mice, an animal model of AD.Results In vivo multiphoton microscopy revealed that 2 month-long administration of NB-02 halted amyloid plaque deposition and cleared amyloid in the cortex. Post-mortem analysis verified NB-02-dependent decrease in plaque deposition in the cortex as well as hippocampus. Furthermore, drug treatment reversed neuronal calcium elevations, thus restoring neuronal function. Finally, NB-02 transformed the morphology of astrocytes and microglia to a more phagocytic state, affecting neuroinflammation. Conclusions NB-02 was effective at reversing AD neuropathophysiology in an animal model. Therefore, in addition to serving as a promising preventative agent, NB-02 holds potential as a treatment for AD in the clinic.
Tight Junction-Mediated Morphologic and Adherent Diversities Act on Hematopoietic Fate Induced by OCT4 in human hair follicle mesenchymal stem cells
Authors: Yu Xiaozhen , Sun Pengpeng , Huang Xingang , Chen Hua , Huang Weiqing , Ruan Yingchun , Jiang Weina , Tan Xiaohua , Liu Zhijing 
Published: July 2020

Abstract
Background: Human hair follicle mesenchymal stem cells (hHFMSCs) isolated from hair follicles possess multilineage differentiation potential. OCT4 is a gene critically associated with pluripotency properties. The cell morphology and adhesion of hHFMSCs significantly changed after transduction of OCT4 and two subpopulations emerged, including adherent cells and floating cell. Floating cells cultured in hematopoietic induction medium and stimulated with erythropoetic growth factors could transdifferentiate into mature erythrocytes, whereas adherent cells formed negligible hematopoietic colonies. The aim of this study was to reveal the role of cell morphology and adhesion on erythropoiesis induced by OCT4 in hHFMSCs and to characterize the molecular mechanisms involved.Methods: Floating cell were separated from adherent cell by centrifugation of the upper medium during cell culture. Cell size was observed through flow cytometry and cell adhesion was tested by disassociation and adhesion assays. RNA sequencing was performed to detect genome-wide transcriptomes and identify differentially expressed genes. GO enrichment analysis and KEGG pathway analysis were performed to analysis the functions and pathways enriched by differentially expressed genes. The expression of tight junction core members was verified by qPCR and Western blot.Results: The overexpression of OCT4 influenced the morphology and adhesion of hHFMSCs. Transcripts in floating cells and adherent cells are quite different. Data analysis showed that upregulated genes in floating cells were mainly related to the pluripotency, germ layer development (including hematopoiesis lineage development), and downregulated genes were mainly related to cell adhesion, cell junctions and the cytoskeleton. Most molecules of the tight junction (TJ) pathway were downregulated and molecular homeostasis of the TJ was disturbed, as CLDNs were disrupted, and JAMs and TJPs were upregulated. The dynamic expression of cell adhesion-related gene E-cadherin and cytoskeleton-related gene ACTN2 might cause different morphology and adhesion. Finally, a regulatory network centered to OCT4 was constructed, which elucidated the TJ pathway critically bridges pluripotency and hematopoiesis in a TJP1-dependent way.Conclusions: Regulations of cell morphology and adhesion via the TJ pathway conducted by OCT4 might modulate hematopoiesis in hHFMSCs, thus developing potential mechanism of erythropoiesis in vitro.
The Challenges and Opportunities of Online Volunteering for COVID-19 Response in Iran: A Qualitative Study
Authors: Seddighi Hamed , Salmani Ibrahim , Ermolaeva Polina , Basheva Olga , Sedeh Mehrab Sharifi 
Published: July 2020

Abstract
Background: The aim of this brief report is to identify the challenges and opportunities of online volunteering to respond to the COVID-19 pandemic from the perspective of the managers of the Iranian Red Crescent Society (IRCS).Methods: In this study we have used a qualitative method including semi-structured interviews for investigating the opportunities and challenges of using virtual volunteers. The eligible participants meeting the inclusion criteria for this study were eight IRCS managers who had used online volunteers for COVID-19 pandemic response.Results: Having considered the interviews with IRCS managers, we finally found seven items as opportunities including safety, availability, recruiting more volunteers, cost reduction, participation, geographical scope, and local considerations. Moreover, five items were found as challenges of online volunteering in IRCS including lack of commitment, cultural issues, infrastructure, reimbursement, and volunteer management.Conclusion: Online volunteering is a significant opportunity for humanitarian organizations especially during epidemics like the current COVID-19. Online volunteering for COVID-19 response by the Red Crescent involved a wide range of people including young people, the elderly, people with disabilities and minority groups, and people in rural areas.
A Report on the Tick Burden, Molecular Detection and Phylogenetic Analysis of Anaplasma Marginale in the Blood Samples of Cattle Collected from District Layyah in Punjab (Pakistan)
Authors: Ashraf Sehrish , Parveen Asia , Asif Muhammad , Awais Mian Muhammad , Khan Adil , Aktas Munir , Ozubek Sezayi , Alanazi Abdullah D , Alyousif Mohamed S , Iqbal Furhan 
Published: July 2020

Abstract
Back ground: Anaplasmosis is known as yellow bag or yellow fever and it is a tick borne disease caused by obligate intercellular gram negative bacteria, Anaplasma marginale. Aim: Present study is reporting seasonal prevalence, epidemiology and phylogeny of Anaplasma marginale in three cattle breeds from District Layyah, Southern Punjab, Pakistan. Methods and results: A total of 844 blood samples (Cross = 300, Holstein Friesian = 244, Sahiwal breed = 300) from apparently healthy cattle on seasonal basis were collected along with epidemiological data. Results: Polymerase chain reaction generated 265 base pair amplicon specific for Major surface protein–1b encoding gene of Anaplasma marginale in 8.6% (73/844) of enrolled cattle. Highest prevalence was observed during autumn (18.3%) followed by summer (9.7%) and winter season (7.1%). Holstein Friesian breed was most susceptible to A. marginale infection (13.1%) followed by Sahiwal (7.6%) and cross breed (6%). Representative amplified partial gene sequences of A. marginale were submitted to GenBank (Accession numbers MK032842 and MK032843). Its phylogenetic analysis had shown similarities with sequences submitted by other countries. 37/844 (4.3%) Giemsa stained blood smears were found positive for Anaplasma spp. Analysis of epidemiological factors revealed that female cattle and farm with water supply from pool, farms where other dairy animals and dogs were living with cattle and dogs having ticks load on them had significant association with A. marginale prevalence. It was observed that white blood cell, lymphocytes (%), Monocytes (%) heamatocrit, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were significantly disturbed in A. marginale positive than negative cattle. Conclusion: We recommend that this PCR protocol should be used for the detection of A. marginale in livestock for their proper diagnosis and treatment.
Colostrum Avoidance Practices and Its Associated Factors Among Mothers of Children Aged Less Than 12 Months in Jinka Town, South Ethiopia, 2019
Authors: Hadona Elias Amaje , W/Hawariat Feleke G/Meskel , Sorrie Muluken Bekele 
Published: July 2020

Abstract
Introduction: Colostrum avoidance is the common nutritional malpractices in developing countries including Ethiopia. Discarding colostrum is the main causes of respiratory disease and diarrheal disease during childhood and contributes to over a million avoidable infant deaths each year and remains a public health problem in developing countries. Despite the fact that it has a negative effect on child health, little is known about the extent of the problem and its contributing factors in South Ethiopia particularly in study area. The aim of the study was to determine the prevalence of colostrum avoidance practices and associated factors among Mothers of children aged less than 12 months in Jinka Town, South Ethiopia. Methods: A community based cross-sectional study was conducted at Jinka Town from March 1 to 30, 2019. A total of 420 mothers having children less than 12 months of age were selected by systematic sampling technique. The data were collected by using pretested interviewer administered and semi-structured questionnaires. The data was entered using EPIDATA 3.1 and exported to SPSS version 23 for analysis. Descriptive statistics, Binary and multivariable logistic regression analysis were done. An adjusted odds ratio with 95% CI at a p-value < 0.05 was estimated to identify statistically significant variables with colostrum avoidance practices. Results: The prevalence of colostrum avoidance practice was 9.8% [95% CI: (6.9–12.5)]. Delayed initiation of breast feeding [AOR= 9.08(95% CI 4.16-19.83)], Lack of breast feeding counselling [AOR= 2.33(95% CI 1.11-4.87)], Home delivery of index child [AOR= 2.48 (95% CI 1.16-5.27)] and Poor knowledge on breast feeding [AOR=4.55(95% CI 1.95-10.63)] were factors associated with colostrum avoidance practices. Conclusions: Colostrum avoidance practice among mothers of children aged less than 12 months in Jinka town was high. Delayed initiation of Breas feeding, lack of breast feeding counselling, Home delivery and knowledge on breastfeeding feeding practice were factors associated with colostrum avoidance practice. Promoting institutional delivery services and strengthening of breast feeding counselling during antenatal and postnatal care were recommended for the promotion of the nutritional value of colostrum and its health benefits.
Typologies of Family Functioning and 24-Hour Movement Behaviors
Authors: Guerrero Michelle , Barnes Joel , Tremblay Mark , Pulkki-Råback Laura 
Published: July 2020

Abstract
Objective: The purpose of the current study was to use latent profile analysis to identify family typologies characterized by parental acceptance, parental monitoring, and family conflict, and to examine whether such typologies were associated with the number of movement behavior recommendations (i.e., physical activity, screen time, and sleep) met by children. Methods: Data for this cross-sectional observational study were part of the baseline data from the Adolescent Brain Cognitive Development (ABCD) study. Data were collected from September 1, 2016 to September 15, 2018, across 21 study sites in the United States. Participants included 11,875 children aged 9 and 10 years. Results: Results from latent profile analysis showed that children were meaningfully classified into one of five family typologies, ranging from ideal (high acceptance, high monitoring, and low conflict) to poor (medium acceptance, low monitoring, and high conflict) functioning. Children from good (OR= 0.54; 95% CI, 0.39-0.76), average (OR=0.28; 95% CI, 0.20, 0.40), fair (OR=0.24; 95% CI, 0.16, 0.36), and poor (OR=0.19; 95% CI, 0.12-0.29) functioning families were less likely to meet all three movement behavior recommendations compared to children from ideal functioning families. The odds of meeting all recommendations progressively decreased as family functioning worsened. Similar findings and pattern of results were found for meeting ≥2 recommendations and ≥1 recommendation. Conclusions: These findings highlight the importance of the family environment for promoting healthy movement behaviors among children.
The COVID-19 Process and the Exchange Rate Relation: An Application on Turkey
Authors: Dineri Eda , Çütçü İbrahim 
Published: July 2020

Abstract
The recent shocks in supply and demand in the world are not due to unexpected economic reasons; in fact, they are related to Covid-19 that causes rapidly spreading global health problems and life threats around the world. While the global powers are dealing with the social problems created by Covid-19 pandemic, they should not neglect the economic changes created by this pandemic. The most important of these economic changes in developing countries with high fragility is exchange rates, because exchange rates can directly affect many macroeconomic variables, from inflation to foreign trade, from the balance of payments to interests. In countries with high fragility due to the effect of pandemic, economic uncertainty causes fluctuations in the exchange rate. Is the reason for the change in the exchange rate, the number of cases or economic risks that may occur due to possible health problems?In this study, the impact of the number of new cases and the number of new deaths for the process of Covid-19 pandemic on the exchange rate in Turkey is examined. The daily data consider the number of new cases, the number of new deaths and exchange rate for the period of 16.03.2020–06.05.2020. The first step of the analysis, the stationary of the series is tested by Lee and Strazicich (2003) unıt root test which allowed structural break. Hatemi-J (2008) Cointegration Test that allow two structural breaks and Hacker-Hatemi-J Bootstrap causality test are used in the analysis. In the results of the Hatemi- J (2008) cointegration test, there is a medium and long-term relationship, with under structural breaks between the number of new cases and the number of new deaths and the exchange rate. According to the results of the analysis, it can be concluded that the number of new cases and the number of new deaths have a significant effect on the exchange rate, causing uncertainty in the economy.JEL Classification: I19, F31, C22