bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial dysfunction and energy deprivation in the mechanism of neurodegeneration
Authors: Abramov Andrey Y. , Angelova Plamena R. 
Published: October 2019

Abstract
Energy-producing organelles mitochondria are involved in a number of cellular functions. Deregulation of mitochondrial function due to mutations or effects of mitochondrial toxins is proven to be a trigger for diverse pathologies, including neurodegenerative disorders. Despite the extensive research done in the last decades, the mechanisms by which mitochondrial dysfunction leads to neuronal deregulation and cell death have not yet been fully elucidated. Brain cells are specifically dependent on mitochondria due to their high energy demands to maintain neuronal ion gradients and signal transduction, and also, to mediate neuronal health through the processes of mitochondrial calcium homeostasis, mitophagy, mitochondrial reactive oxygen species production and mitochondrial dynamics. Some of these processes have been independently implicated in the mechanism of neuronal loss in neurodegeneration. Moreover, it is increasingly recognised that these processes are interdependent and interact within the mitochondria to ensure proper neuronal function and survival.
Kombine flaş ikili jeotermal santrali ve entegre hidrojen üretim sisteminin yaşam döngü maliyet analizi
Authors: YILMAZ Ceyhun 
Published: October 2019

&nbsp;Bu
çalışmanın amacı, hidrojen üretiminde jeotermal enerjinin kullanımı için
bilgisayar ortamında termodinamik olarak modellenen bir sistemin kapsamlı bir
şekilde termodinamik ve ekonomik yöntemler yardımıyla incelenmesi, optimize
edilmesi ve yaşam döngü maliyet analizinin yapılmasıdır. Bu çalışmada sıvı bir
jeotermal kaynak elektrik üretimlinde kullanılmış ve bu elektrik elektroliz
ünitesinde suyun hidrojen ve oksijene parçalanmasında, iş girdisi olarak
kullanılmıştır. Gerekli termodinamik kabuller yapılarak, sistem bilgisayar
ortamında bir simülasyon programıyla çalıştırıldığında jeotermal güç
çevriminden elde edilen maksimum net iş 8063 kW’tır. Üretilen bu elektriğin
birim enerji maliyeti 0,0107 $/kWh. Üretilen elektriğin doğrudan elektroliz
ünitesinde hidrojen üretilmesi için kullanılması ile sistemden üretilen
hidrojenin debisi 0,053 kg/s olarak hesaplanmıştır. Üretilen hidrojenin birim
maliyeti ise 2.767 $/kg H2 olarak hesaplanmıştır. Sistemin yaşam
döngü maliyet analizi sonucunda, Net Bugünkü Değeri (NPV) 35.340.000 $ olarak
hesaplanmıştır. Levelize edilmiş yıllık maliyet metodu ile seviyelendirilmiş
yıllık maliyeti ise (LAC) 3.395.000 $/yr olarak hesaplanmıştır. Sistemin basit
geri ödeme süresi (Nsbp) 4.047 yıl olarak hesaplanmıştır.
İndirgenmiş geri ödeme süresi ise (Ndbp) 5.442 yıl olarak
hesaplanmıştır.
Velocity Structure at the Source Physics Experiment Phase I Site Obtained with the Large‐N Array Data
Authors: Chen Ting , Snelson Catherine M. , Mellors Robert 
Published: October 2019

Abstract
The Source Physics Experiment (SPE) consists of a series of chemical explosions at the Nevada National Security Site. The goal of the SPE is to understand and model seismic‐wave generation and propagation from these explosions. To achieve this goal, we need an accurate velocity model of the SPE site. A large‐N seismic array deployed at the SPE site during one of the chemical explosions (SPE‐5) provides great data for this purpose. The array consists of 996 geophones and covers an area of approximately 2×2.5  km. In addition to the SPE‐5 explosion, the array recorded 53 large weight drops. Using the large‐N seismic array recordings, we perform first‐arrival analysis and obtain a 2D P‐wave velocity model of the SPE site. We image a sharp transition from high‐velocity Cretaceous granite to low‐velocity Quaternary alluvium. Other geological units such as the Tertiary volcanic rocks and Paleozoic sedimentary rocks are also clearly shown. The results of this work provide important local geological information and will be incorporated into the larger 3D modeling effort of the SPE program to validate the predictive models developed for the site.
Barriers to the Provision of Spiritual Care by Nurses for Hospitalized Patients in Jordan
Authors: Musa Ahmad Salem , Al Qadire Mohammad Ibrahim , Aljezawi Ma'en , Tawalbeh Loai I. , Aloush Sami , Albanian Fayez Z. 
Published: October 2019

Background and PurposeSpiritual care is an essential part of holistic patient care but is provided infrequently in practice. There is a paucity of research investigating the barriers to the provision of spiritual care among Jordanian nurses. The main purpose of this cross-sectional, correlational study was to identify these barriers and to explore the associations with nurses' personal and professional characteristics. A secondary purpose was to examine the psychometric properties of a newlydeveloped spiritual care barriers instrument.MethodsWe surveyed a convenience sample of Jordanian nurses (N = 282). Participants completed both the Spiritual Well-Being Scale (SWBS) and the Spiritual Care Barriers Scale (SCBS). We conducted an exploratory factor analysis to examine the internal structure of the SCBS, and internal consistency was assessed by Cronbach's alpha.ResultsThe most common perceived barriers were lack of private places (82.3%), absence of an imam (79.8%), insufficient time (78.8%), inadequate skills and competencies (73.7%), lack ofreligious and spiritual facilities and resources in the hospital (71.3%), and insufficient knowledge (71.3%). Nurses' personal spiritual and existential well-being were negatively associated with spiritual care barriers. Head nurses and supervisors reported lower mean scores on perceived barriers than did associate nurses. The SCBS exhibited acceptable evidence of internal consistency and validity.Implications for PracticeThe findings help Jordanian nurse decision makers in practice and education to overcome barriers to the provision of spiritual care to better meet the spiritual needs of Muslim patients.
Aggressive and cannibalistic behavior of African catfish larvae: effect of different doses of methyltestosteron injecting to female broodstock and larval stocking densities
Authors: Rahmadiah Triayu , Junior Muhammad Zairin , Alimuddin Alimuddin , Diatin Iis 
Published: October 2019

ABSTRACT This study aimed to evaluate the effect of 17α-methyltestosterone hormone (MT) injecting to female broodstocks and stocking densities on the aggressive and cannibalistic behavior of African catfish larvae. Two-day-old post-hatching larvae were used in this experiment. Larval rearing was started at three-day-old post-hatching (body weight 0.004 ± 0.003 g and total length 0.2 ± 0.05 cm) in a 40 cm×30 cm× 25 cm rearing aquaria. This study used a completed randomized factorial design which consisted of two factors. The first factor were the larvae from females broodstocks without MT injection (0 μg/g body weight) (A), injected with MT (1 μg/g body weight) (B), and injected with MT (2 μg/g body weight) (C), while the second factor were larval stocking densities of 3.000/m2 (V1) and 6.000/m2 (V2), with three replications. The results showed that the larvae from female broodstocks injected with MT 2 μg/g body weight (C) and stocking densities 6.000/m2 (V2) increased the aggressiveness of swim and decreased cannibalism. The highest cannibalistic behavior occurred from 00.00‒06.00.Keyword: aggressiveness, cannibalism, Clarias gariepinus, methyltestosterone ABSTRAKPenelitian ini bertujuan untuk mengevaluasi pengaruh hormon 17α-metiltestosteron (MT) yang diinjeksikan pada induk betina dan padat tebar terhadap tingkat agresivitas dan kanibalisme larva ikan lele. Ikan uji yang digunakan adalah larva berumur dua hari setelah penetasan. Pemeliharaan larva dimulai saat larva berumur tiga hari setelah penetasan (bobot tubuh 0,004 ± 0,003 g dan panjang total 0.2 ± 0.05 cm) yang dipelihara di dalam akuarium berukuran 40 cm×30 cm×25 cm. Penelitian ini menggunakan rancangan acak lengkap faktorial yang terdiri atas dua faktor. Faktor yang pertama adalah larva dari induk tanpa diinjeksi dengan hormon MT (0 μg/g bobot tubuh) (A), diinjeksi dengan hormon MT (1 μg/g bobot tubuh) (B), dan diinjeksi dengan hormon MT (2 μg/g bobot tubuh) (C), sedangkan faktor yang kedua adalah padat tebar 3.000 ekor/m² (V1) dan 6.000 ekor/m² (V2), dengan tiga kali ulangan. Hasil penelitian menunjukkan bahwa larva ikan lele dari induk yang diinjeksi hormon MT dosis 2 µg/g bobot tubuh dan dipelihara pada padat tebar tinggi 6.000 ekor/m² (V2) dapat meningkatkan agresivitas berenang dan menurunkan kanibalisme. Tingkat kanibalisme tertinggi terjadi pada pukul 24.00‒06.00 WIB. Kata kunci: agresivitas, kanibalisme, Clarias gariepinus, metiltestosteron
Origin and distribution of dolomite in Permian Rotliegend siliciclastic sandstones (Dutch Southern Permian Basin)
Authors: Molenaar Nicolaas , Felder Marita 
Published: October 2019

ABSTRACTDolomite is a common and volumetrically important mineral in many siliciclastic sandstones, including Permian Rotliegend sandstones (the Slochteren Formation). These sandstones form extensive gas reservoirs in the Southern Permian Basin in the Netherlands, Germany, Poland, and the UK. The reservoir quality of these sandstones is negatively influenced by the content and distribution of dolomite. The origin and the stratigraphic distribution of the dolomite is not yet fully understood. The aim of this study is to identify the origin of carbonate. The main methods used to achieve those aims are a combination of thin-section petrography, scanning electron microscopy (SEM and EDX), and XRD analyses.The present study shows that the typical dispersed occurrence of the dolomite is a consequence of dispersed detrital carbonate grains that served both as nuclei and source for authigenic dolomite cement. The dolomite cement formed syntaxial outgrowths and overgrowths around detrital carbonate grains. The study also shows that dolomite cement, often in combination with ankerite and siderite, precipitated during burial after mechanical compaction. Most of the carbonate grains consisted of dolomite before deposition. The carbonate grains were affected by compaction and pressure dissolution, and commonly have no well-defined outlines anymore.The distribution of dolomite cement in the Rotliegend sandstones was controlled by the presence of stable carbonate grains. Due to the restricted and variable content of carbonate grains and their dispersed occurrence, the cement is also dispersed and the degree of cementation heterogeneous.Our findings have important implications on diagenesis modeling. The presence of detrital carbonate excludes the need for external supply by any large-scale advective flow of diagenetic fluids. By knowing that the carbonate source is local and related to detrital grains instead of being externally derived from an unknown source, the presence of carbonate cement can be linked to a paleogeographic and sedimentological model.
Gene Loss and Evolution of the Plastome
Authors: Mohanta Tapan Kumar , Khan Adil , Khan Abdul Latif , Hashem Abeer , Abd_Allah Elsayed Fathi , Al-Harrasi Ahmed 
Published: October 2019

Abstract
Chloroplasts are unique organelles within plant cells and are ultimately responsible for sustaining life forms on the earth due to their ability to conduct photosynthesis. Multiple functional genes within the chloroplast are responsible for a variety of metabolic processes that occur in the chloroplast. Considering its fundamental role in sustaining life on earth, it is important to identify the level of diversity present in the chloroplast genome, what genes and genomic content have been lost, what genes have been transferred to the nuclear genome, duplication events, and the overall origin and evolution of the chloroplast genome. Our analysis of 2511 chloroplast genomes indicated that the genome size and number of CDS in the chloroplasts of algae are higher relative to other lineages. Approximately 10.31% of the examined species have lost the inverted repeats (IR) that span across the lineages that comprise algae, bryophytes, pteridophytes, gymnosperm, angiosperms, magnoliids, and protists. Genome-wide analyses revealed that the loss of the RBCL gene in parasitic and heterotrophic plant species occurred approximately 56 Ma ago. PsaM, Psb30, ChlB, ChlL, ChlN, and RPL21 were found to be characteristic signature genes of chloroplast genome of algae, bryophytes, pteridophytes, and gymnosperms; while none of these genes were found in the angiosperm or magnoliid lineage which appeared to have lost them approximately 203-156 Ma ago. A variety of chloroplast encoding genes were lost across different species lineages throughout the evolutionary process. The Rpl20 gene, however, was found to be the most stable and intact gene in the chloroplast genome and was not lost in any of the analysed species; suggesting that it is a signature gene of the plastome. Our evolutionary analysis indicated that chloroplast genomes evolved from multiple common ancestors ~1293 Ma ago and have undergone vivid recombination events across different taxonomic lineages. Additionally, our findings support the hypothesis that these recombination events are the most probable cause behind the dynamic loss of chloroplast genes and inverted repeats in different species.
In silico Discovery of Resveratrol Analogues as Potential Agents in Treatment of Metabolic Disorders
Authors: Pavlović Nebojša , Đanić Maja , Stanimirov Bojan , Goločorbin-Kon Svetlana , Stankov Karmen , Lalić-Popović Mladena , Mikov Momir 
Published: October 2019

Background:
Resveratrol was demonstrated to act as partial agonist of PPAR-γ receptor, which opens
up the possibility for its use in the treatment of metabolic disorders. Considering the poor bioavailability of resveratrol,
particularly due to its low aqueous solubility, we aimed to identify analogues of resveratrol with improved
pharmacokinetic properties and higher binding affinities towards PPAR-γ.

Methods:
3D structures of resveratrol and its analogues were retrieved from ZINC database, while PPAR-γ structure
was obtained from Protein Data Bank. Docking studies were performed using Molegro Virtual Docker software.
Molecular descriptors relevant to pharmacokinetics were calculated from ligand structures using VolSurf+
software.

Results:
Using structural similarity search method, 56 analogues of resveratrol were identified and subjected to
docking analyses. Binding energies were ranged from -136.69 to -90.89 kcal/mol, with 16 analogues having
higher affinities towards PPAR-γ in comparison to resveratrol. From the calculated values of SOLY descriptor,
23 studied compounds were shown to be more soluble in water than resveratrol. However, only two tetrahydroxy
stilbene derivatives, piceatannol and oxyresveratrol, had both better solubility and affinity towards PPAR-γ.
These compounds also had more favorable ADME profile, since they were shown to be more metabolically stable
and wider distributed in body than resveratrol.

Conclusion:
Piceatannol and oxyresveratrol should be considered as potential lead compounds for further drug
development. Although experimental validation of obtained in silico results is required, this work can be considered
as a step toward the discovery of new natural and safe drugs in treatment of metabolic disorders.
TRIM45 Suppresses the Development of Non-small Cell Lung Cancer
Authors: Peng Xiyang , Wen Yao , Zha Lagabaiyila , Zhuang Jian , Lin Li , Li Xu , Chen Yu , Liu Zhiqiang , Zhu Shuangli , Liang Jifeng , Zhou Zuoqiong , Yuan Wuzhou , Li Yongqing , Wang Yuequn , Jiang Zhigang , Mo Xiaoyang , Wan Yongqi , Shi Yan , Zhu Ping , Zheng Lan , Tang Changfa , Wu Xiushan , Fan Xiongwei 
Published: October 2019

Background:
Previously, we first identified the human tripartite motifcontaining
protein 45 (TRIM45) acts as a novel transcriptional repressor in mitogenactivated
protein kinase (MAPK) signaling pathway. After that, the inhibitory role of
TRIM45 in the development of tumor was gradually unveiled. However, the function of
TRIM45 in the tumorigenesis of lung cancer has not been characterized.

Methods and Results:
In this study, we found that TRIM45 was up-regulated in earlystage
human non-small-cell lung cancer (NSCLC) tissues. Overexpression of TRIM45 in
lung cancer cells induces G1 arrest and promotes apoptosis, which accompanied by upregulated
expression of RB, p16, p53, p27Kip1, and Caspase3 and down-regulated expression
of CyclinE1 and CyclinE2. Further detection of the expression of the molecules
in the MAPK signaling pathway revealed that overexpression of TRIM45 in lung cancer
cells promotes phosphorylated p38 (p-p38) activation and inhibits phosphorylated ERK
(p-ERK) activation. In accordance with this, p-p38 is increased while p-ERK is decreased
in lung cancer tissues.

Conclusion:
These findings indicate that TRIM45 plays an inhibitory role in the tumorigenesis
of lung cancer. High-level expression of TRIM45 in lung cancer tissue may promote
cell apoptosis by activating p38 signal and inhibit proliferation by down-regulating
p-ERK, which provides a new clue for understanding the tumorigenesis of lung cancer.
Overexpression of Pygo2 Increases Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Cardiomyocyte-like Cells
Authors: Yang Lei , Zhu Shuoji , Li Yongqing , Zhuang Jian , Chen Jimei , Huang Huanlei , Chen Yu , Wen Yulin , Wen Yao , Guo Huiming , Fan Xiongwei , Yuan Wuzhou , Jiang Zhigang , Wang Yuequn , Wu Xiushan , Zhu Ping 
Published: October 2019

Background:
Our previous studies have shown that Pygo (Pygopus) in Drosophila plays
a critical role in adult heart function that is likely conserved in mammals. However, its role in the
differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into cardiomyocytes
remains unknown.

Objective:
To investigate the role of pygo2 in the differentiation of hUC-MSCs into cardiomyocytes.

Methods:
Third passage hUC-MSCs were divided into two groups: a p+ group infected with the
GV492-pygo2 virus and a p− group infected with the GV492 virus. After infection and 3 or 21 days
of incubation, Quantitative real-time PCR (qRT-PCR) was performed to detect pluripotency
markers, including OCT-4 and SOX2. Nkx2.5, Gata-4 and cTnT were detected by immunofluorescence
at 7, 14 and 21 days post-infection, respectively. Expression of cardiac-related
genes—including Nkx2.5, Gata-4, TNNT2, MEF2c, ISL-1, FOXH1, KDR, αMHC and α-Actin—were
analyzed by qRT-PCR following transfection with the virus at one, two and three weeks.

Results :
After three days of incubation, there were no significant changes in the expression of the
pluripotency stem cell markers OCT-4 and SOX2 in the p+ group hUC-MSCs relative to controls
(OCT-4: 1.03 ± 0.096 VS 1, P > 0.05, SOX2: 1.071 ± 0.189 VS 1, P > 0.05); however, after 21
days, significant decreases were observed (OCT-4: 0.164 ± 0.098 VS 1, P < 0.01, SOX2: 0.209 ±
0.109 VS 1, P < 0.001). Seven days following incubation, expression of mesoderm specialisation
markers, such as Nkx2.5, Gata-4, MEF2c and KDR, were increased; at 14 days following
incubation, expression of cardiac genes, such as Nkx2.5, Gata-4, TNNT2, MEF2c, ISL-1, FOXH1,
KDR, αMHC and α-Actin, were significantly upregulated in the p+ group relative to the p− group (P
< 0.05). Taken together, these findings suggest that overexpression of pygo2 results in more hUCMSCs
gradually differentiating into cardiomyocyte-like cells.

Conclusion:
We are the first to show that overexpression of pygo2 significantly enhances the
expression of cardiac-genic genes, including Nkx2.5 and Gata-4, and promotes the differentiation of
hUC-MSCs into cardiomyocyte-like cells.
Predictive Equations Based on Body Composition for Resting Energy Expenditure Estimation in Adults with Obesity
Authors: El Masri Dana , Itani Leila , Kreidieh Dima , Tannir Hana , El Ghoch Marwan 
Published: October 2019

Background and Aim:An accurate estimation of Resting Energy Expenditure (REE) in patients with obesity is crucial. Therefore, our aim was to assess the validity of REE predictive equations based on body composition variables in treatment-seeking Arab adults with obesity.Methods:Body composition and REE were measured by Tanita BC-418 bioimpedance and Vmax Encore 229 IC, respectively, and predictive equations based on fat mass and fat-free mass were used in REE estimations among 87 adults of both genders, in the Outpatient Clinic in the Department of Nutrition and Dietetics at Beirut Arab University (Lebanon). The mean differences between the measured and estimated REE values were calculated to assess the accuracy, and the Bland-Altman method was used to assess the level of agreement.Results:Ten predictive equations were included. In males, all the predictive equations gave significantly different estimates of REE when compared to that measured by IC. On the other hand, in females, the mean difference between the REE value estimated by Huang and Horie-Waitzberg equations and that measured using IC was not significant, and the agreement was confirmed using Bland-Altman plots.Conclusion:Huang and Horie-Waitzberg equations are suggested for accurate REE estimation in females; however, new validated REE estimation equations for males in this population are still needed.
ЕФЕКТИВНІСТЬ БІОФЛАВОНОЇДІВ ПРИ ЛІКУВАННІ ХВОРИХ НА ПОДАГРУ НА ТЛІ МЕТАБОЛІЧНОГО СИНДРОМУ
Authors: Якименко О. О. , Гриценко М. В. 
Published: October 2019

Резюме. Метою дослідження було оцінити ефективність застосування біофлавоноїду кверцетину у хворих на подагру на тлі метаболічного синдрому.
Об’єкт і методи дослідження. Обстежено 61 пацієнта з первинною подагрою в поєднанні з метаболічним синдромом та виділені групи пацієнтів. Основній групі (n=30) було призначено алопуринол (початкова доза 100 мг на добу з наступним титруванням дози до підтримуючої) та квертин 40мг по 1 таблетці 3 рази на добу протягом 6 місяців, з повторенням курсу через 2-3 місяці. Групі порівняння (n=31) тільки алопуринол. Схема лікування була призначена у період ремісії. Оцінку ефективності проводили через 12 та 24 місяці за допомогою лабораторних методів дослідження.
Результати. При динамічному спостереженні на фоні лікування відмічалася тенденція до зниження концентрації сечової кислоти через 12 місяців на 38,35 % в першій групі та на 23 % в другій групі, а через 24 місяці на 49,12 % в першій групі та на 36,68 % в другій групі. Через 12 та 24 місяці відмічалося статистично достовірне зниження С-реактивного протеїну та сіромукоїдів в основній групі, в той же час фібриноген та швидкість осідання еритроцитів мали тенденцію до зниження, але різниця була статистично недостовірною. При оцінці функції нирок відмічалося достовірне зниження концентрації креатиніну та сечовини та збільшення швидкості клубочкової фільтрації в основній групі. Також у групі 1 достовірно знизився рівень холестерину ліпопротеїнів низької щільності через 12 місяців та 24 місяці та підвищився рівень холестерину ліпопротеїнів високої щільності; щодо рівня загального холестерину та тригліцеридів, то відмічалася тенденція до зниження цих показників. В той же час у групі 2 суттєвих відмінностей не було.