bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNAH19 improves insulin resistance in skeletal muscle by regulating heterogeneous nuclear ribonucleoprotein A1
Authors: Gui Weiwei , Zhu WeiFen , Zhu Yiyi , Tang Shengjie , Zheng Fenping , Yin Xueyao , Lin Xihua , Li Hong 
Published: June 2020

Abstract
Background
Skeletal muscle is essential for glucose and lipid metabolism. Growing evidence reveals the importance of long non-coding RNAs (LncRNAs) in metabolism. This study aimed to investigate the function of LncRNA H19 (H19) in lipid metabolism of skeletal muscle and its potential mechanisms.
Methods
Glucose tolerance, serum insulin and lipid content in serum and skeletal muscle were determined in control and H19-overexpressed db/db mice. Lipid metabolism was evaluated in H19-overexpressed or H19-silencing muscle cells by detecting lipid contents and mitochondria related functions. The underlying mechanisms were explored by RNA pull-down, mass spectrometry and RNA immunoprecipitation (RIP).
Results
H19 was downregulated in skeletal muscle of db/db mice. H19 overexpression in db/db mice inhibited lipid ectopic deposition in skeletal muscle, meanwhile improved glucose intolerance and insulin resistance as compared with control db/db mice treated with ad-GFP. Furthermore, overexpression of H19 reversed FFA-induced lipid accumulation and increased cellular respiration in muscle cells, while H19 knockdown exhibited opposite effects in muscle cells. Mechanistically, H19 interacted with heterogeneous nuclear ribonucleoprotein (hnRNPA1) which was validated by RNA pulldown and RIP analysis, which increased translation of fatty acid oxidation closely related genes PGC1a and CPT1b.
Conclusion
Our data suggest that overexpression of H19 ameliorates insulin resistance by reducing ectopic lipid accumulation in skeletal muscle. The possible underlying mechanisms are that overexpression of lncRNAH19 promotes fatty acids oxidation via targeting of hnRNPA1.
Luks-pv Induces Apoptosis Through Methyltransferase Set8 in Human Acute Myeloid Leukaemia Cells
Authors: Xu Liangfei , Shi Lan , Ding Pengsheng , Ma Fan , Wang Yangyan , Liu Xueer , Song Kaidi , Qiang Ping , Chang Wenjiao , Dai Yuanyuan , Mei Yide , Ma xiaoling 
Published: June 2020

Abstract
Background We previously reported that LukS-PV induces apoptosis in human acute myeloid leukaemia (AML) cells. Furthermore, SET8 is a member of the SET domain-containing methyltransferase family and overexpressed in numerous tumours, including AML and indicated a poor prognosis. However, it is unclear whether LukS-PV induce apoptosis via SET8. In current study, we aimed to investigate the regulatory mechanisms of SET8 and effects on AML cells under the LukS-PV.Methods Flow cytometry was performed to detect apoptosis in AML primary blasts/cell lines treated with LukS-PV and invasion of AML cells in vivo. The expression of SET8 was quantified through reverse-transcription PCR and western blotting. Chromatin-immunoprecipitation (ChIP) sequencing and bioinformatic methods was performed to explore transcriptional target genes which regulated by SET8-H4K20me1 axis. All experiments were performed in triplicate, and all statistical analyses were conducted using SPSS 16.0.Results In this research, we found that LukS-PV induce cell apoptosis in vitro and inhibit cell invasion in vivo. Our results further confirmed SET8 and H4K20me1 were downregulated in LukS-PV-treated AML cells. Furthermore, we confirmed that LukS-PV induced apoptosis via downregulating SET8/H4K20me1. Finally, Genome-wide analysis identified PIK3CB is the target gene for cell apoptosis mediated by SET8/H4K20me1. In a nutshell, LukS-PV induces apoptosis via the PIK3CB/PI3K/AKT/FOXO1 signal pathway by targeting SET8.Conclusions The present results indicate that LukS-PV induces apoptosis in AML cells by downregulating SET8 and regulating downstream molecular targets, suggesting that SET8 is a potential target for AML therapy using LukS-PV for anti-leukaemia treatment.
A One Health Framework to Estimate Costs of Antimicrobial Resistance
Authors: Morel Chantal , Alm Richard , Ardal Christine , Bandera Alessandra , Bruno Giacomo , Carrara Elena , Colombo Giorgio , de Kraker Marlieke , Essack Sabiha , Frost Isabel , Gonzalez-Zorn Herman Bruno , Guardabassi Luca , Harbarth Stephan , Jørgensen Peter S. , Kanj Souha , Kostyanev Tomislav , Laxminarayan Ramanan , Leonard Finola , Hara Gabriel Levy , Mendelson Marc , Mikulska Malgorzata , Mutter Nico , Outterson Kevin , Baňo Jesus Rodriguez , Scudeller Luigia , Tacconelli Evelina 
Published: June 2020

Abstract
Objectives/PurposeThe costs attributable to antimicrobial resistance (AMR) across human, animal, and environmental health remain theoretical and largely unspecified. Current figures fail to capture the full health and economic burden caused by AMR; historically many studies have considered only direct costs associated with human infection from a hospital perspective, primarily from high-income countries. The Global Antimicrobial Resistance Platform for ONE-Burden Estimates (GAP-ON€) network aimed to develop a global, One Health AMR Cost Framework, that can be used by countries, regions, and other entities to quantify the economic burden attributable to AMR.MethodsGAP-ON€ (funded under the JPIAMR 8th call (Virtual Research Institute) is composed of 19 international networks and institutions active in the field of AMR. It operated by means of Delphi rounds, teleconferences (TCs) and face-to-face (F2F) meetings. It assumes a bottom-up cost estimate, at the National level, with a global scope across all One Health Areas.ResultsThe resulting framework consists of the epidemiological data and the direct and indirect cost components, linked by the likelihood of each AMR-related health state that imposes such costs, across all One Health Areas for a large number of relevant pathogens. It represents a first step towards more comprehensive analyses for comparing cost-effectiveness of AMR interventions at the local level as well as more harmonised analyses at the global level.ConclusionThis is the first full AMR costing framework to be developed for bacterial pathogens in human and animal health and the environment. It aims to provide guidance on the data items necessary to calculate AMR costs in a global, One Health perspective to facilitate harmonized, full economic analyses. It is only through building a realistic cost picture that we can make informed decisions about potential strategies and political priorities.
Ultra-Low Dose Amiodarone Reduces Tumor Growth and Angiogenesis
Authors: Steinberg Eliana , Fluksman Arnon , Zemmour Chalom , Karsch-Bluman Adi , Brill-Karniely Yifat , Tischenko Katerina , Birsner Amy E , D'Amato Robert J , Benny Ofra 
Published: June 2020

Abstract
Background: Pre-clinical studies suggest that Amiodarone induces cytotoxicity in several types of cancer cells, thus making it a potential candidate for use as an anti-cancer treatment. In this study, we examined Amiodarone's effects on glioblastoma multiforme (GBM), a highly aggressive and hypervascularized cancer. We hypothesized that Amiodarone would show an anti-angiogenic effect on GBM in addition to its previously suggested anti-cancer activity, and that an ultra-low dose would be both effective and possibly avert the drug’s side effects. Methods: The anti-cancer activity of Amiodarone was assessed by several in vitro assays using GBM cells. This included cytotoxicity, proliferation, transwell migration, Anoikis, colony-formation and three-dimensional (3D) spheroid growth assays. The anti-angiogenic effect of Amiodarone was tested on endothelial cells, using toxicity, proliferation, migration and tube formation in vitro assays. The anti-cancer and anti-angiogenic activity of Amiodarone was examined in vivo on three different murine models. C57BL/6J mice were utilized for the corneal neovascularization model and the Matrigel plug assay. Foxn1 nu mice were inoculated with GBM cells and used for the GBM tumor xenograft model.Results: In this study, we showed that Amiodarone has a significant anti-cancer and anti-angiogenic activity in vitro. Moreover, ultra-low dose Amiodarone markedly reduced the size of GBM xenograft tumors and displayed a strong anti-angiogenic effect in vivo. Conclusions: Our results strongly suggest that Amiodarone possess dual cancer fighting properties.
Long Non-Coding RNA HCP5 Functions as A Sponger of miR-29b-3p and Promotes Cell Growth and Metastasis in Hepatocellular Carcinoma Through Upregulating DNMT3A
Authors: Zhou Yongping , Li Kuan , Dai Tu , Wang Hong , Hua Zhiyuan , Bian Wuyang , Wang Hao , Chen Fangming , Ai Xiaoming 
Published: June 2020

Abstract
Background: Multiple researches have revealed that long non-coding RNA (lncRNAs) served as regulatory factor in modulating tumorigenesis of hepatocellular carcinoma (HCC). In this study, we demonstrated that lncRNA HCP5 was a tumor promoting factor of HCC, and the molecular mechanism of HCP5 has been elucidated at length.Methods: BrdU, transwell assay and flow cytometry were exerted to estimate cell growth, metastasis, invasion and apoptosis of HCC in vitro. Animal studies were performed the effect of HCP5 in vivo. Furthermore, we identified that miR-29b-3p interacted with HCP5 or DNA methyltransferase 3A (DNMT3A) using dual luciferase assay. qRT-PCR, western blot, BrdU, tanswell and flow cytometry assays were used to investigate the correlation of HCP5/miR-29b-3p/DNMT3A axis. Results: In present research, we elaborated that HCP5 was overexpressed in HCC tissues and cell lines, and this phenomenon was even obvious in metastatic and recurrent cases. Knockdown of HCP5 significantly alleviated cell growth, metastasis and invasion through preventing apoptosis and activating EMT progress. Moreover, miR-29b-3p has been identified as a negatively regulatory target gene of HCP5, which served as tumor suppressor of HCC to prevent cell proliferation, migration and invasion. Subsequently, DNMT3A was found as a downstream regulatory factor of miR-29b-3p, of which study found that a participated element of HCC progression by activating AKT phosphorylation.Conclusion: Generally, our study elucidated for the first time that HCP5 plays a crucial role in HCC via HCP5/ miR-29b-3p/DNMT3A/AKT axis and demonstrated a novel diagnostic and therapeutic strategy with potentiality.
Use of wearable sensors to assess compliance of asthmatic children in response to lockdown measures for the COVID-19 epidemic
Authors: Kouis Panayiotis , Michanikou Antonis , Anagnostopoulou Pinelopi , Galanakis Emmanouil , Michaelidou Eleni , Dimitriou Helen , Matthaiou Andreas , Kinni Paraskevi , Achilleos Souzana , Zacharatos Harris , Papatheodorou Stefania , Koutrakis Petros , Nikolopoulos Georgios K. , Yiallouros Panayiotis 
Published: June 2020

Abstract
Between March and April 2020, Cyprus and Greece health authorities enforced three escalated levels of public health interventions to control the COVID-19 pandemic. We quantified compliance of asthmatic schoolchildren from both countries to intervention levels, using wearable sensors to continuously track personal location and physical activity. Changes in ‘fraction time spent at home’ and ‘total steps/day’ were assessed with a mixed-effects model adjusting for confounders. We observed significant mean increases in ‘fraction time spent at home’ in Cyprus and Greece, during each intervention level by 41.4% and 14.3% (level 1), 48.7% and 23.1% (level 2) and 45.2% and 32.0% (level 3), respectively. Physical activity in Cyprus and Greece demonstrated significant mean decreases by -2,531 and -1,191 (level 1), -3,638 and -2,337 (level 2) and -3,644 and -1,961 (level 3) total steps/day, respectively. We also report significant independent effects of weekends and age on fraction time spent at home and weekends, age, humidity and gender on physical activity. We suggest that wearable technology provides objective, continuous, real-time location and activity data making possible to inform in a timely manner public health ofﬁcials on compliance to various tiers of public health interventions during a pandemic.
Overcoming Limitations in the Availability of Swabs Systems Used for SARS-CoV-2 Laboratory Diagnostics
Authors: Nairz Manfred , Bellmann-Weiler Rosa , Ladstätter Miriam , Schüllner Falko , Zimmermann Martina , Koller Anna-Maria , Blunder Silvia , Naschberger Helene , Klotz Werner , Herold Manfred , Kerndler Sylvia , Jeske Martina , Haschka David , Petzer Verena , Schroll Andrea , Sonnweber Thomas , Tancevski Ivan , Fritsche Gernot , Araujo Mariana E. G. de , Stasyk Taras , Huber Lukas A. , Griesmacher Andrea , Theurl Igor , Weiss Günter 
Published: June 2020

Abstract
The diagnosis of COVID-19 relies on the direct detection of SARS-CoV-2 RNA in respiratory specimens by RT-PCR. The pandemic spread of the disease caused an imbalance between demand and supply of materials and reagents needed for diagnostic purposes including swab sets. In a comparative effectiveness study, we conducted serial follow-up swabs in hospitalized laboratory-confirmed COVID-19 patients. We assessed the diagnostic performance of an in-house system developed according to recommendations by the US CDC. In a total of 96 swabs, we found significant differences in the accuracy of the different swab systems to generate a positive result in SARS-CoV-2 RT-PCR, ranging from around 50 to 80%. Of note, an in-house swab system was superior to most commercially available sets as reflected by significantly lower Ct values of viral genes. Thus, a simple combination of broadly available materials may enable diagnostic laboratories to bypass global limitations in the supply of swab sets.
Intravenous administration of tranexamic acid to reduce blood loss during perioperative period in acetabular fracture surgery
Authors: Wang ZhiDong , Wang ZhenHeng , Yang Huilin , Zhu RuoFu , Chen GuangDong , Li Mao , Shi WeiDong 
Published: June 2020

Abstract
Backgroud:

some studys have reported that topical use of TXA can reduce perioperative blood loss in acetabular fracture surgery. We aim to investigate the effect of intravenous administration of tranexamic acid (TXA) on perioperative blood loss during acetabular fracture surgery
Methods

From January 2016 to May 2020, 85 patients undergoing surgical treatment with intravenous TXA administration for acetabular fractures at our department were retrospectively analyzed. The patients were divided into three groups according to dosage of TXA: Single treatment group: patients receiving intravenous infusion of TXA (15 mg/kg) 20 min before surgery (n = 33), repeated treatment group: patients receiving intravenous infusion of TXA (15 mg/kg) 20 min before surgery and intravenous infusion of TXA (10 mg/kg) at 3 h (n = 26), and multiple treatment group: patients receiving intravenous infusion of TXA (15 mg/kg) 20 min before surgery and intravenous infusion of TXA (10 mg/kg) at 3 and 6 h (n = 26). Total blood loss, intraoperative blood loss, postoperative hemoglobin drop, surgery-related transfusion rate, postoperative thrombosis rate, and operation time were compared among these three groups
Results

Total blood loss, intraoperative blood loss, postoperative hemoglobin drop, and drainage volume in the single treatment group, repeated treatment group, and multiple treatment group were 932.7.0 ± 181.8 ml, 624.2 ± 138.7 ml, 32.2 ± 5.3 g/l, and 100.1 ± 30.1 ml; 843.4 ± 153.0 ml, 567.3 ± 144.1 ml, 27.6 ± 3.8 g/l, and 86.1 ± 42.2 ml; and 748.0 ± 145.2 ml, 521.1 ± 98.1 ml, 24.4 ± 4.4 g/l, and 64.8 ± 29.0 ml, respectively; the values were significantly different between groups (P < 0.05). The surgery-related blood transfusion rates in the single treatment, repeated treatment, and multiple treatment groups were 51.5% (17/33), 23.0% (6/26), 19.2% (5/26), respectively. There was no statistically significant difference in surgery-related blood transfusion rates between groups
Conclusions

Intravenous administration of TXA in acetabular fracture surgery can reduce total blood loss, intraoperative blood loss, and postoperative hemoglobin drop without increasing the risk of venous thrombosis. Multiple administrations before surgery, and at 3 h and 6 h during surgery are more effective than single and repeated administration
O papel do (a) Psicólogo (a) na Unidade Básica de Saúde sob uma Perspectiva da Psicologia da Saúde
Authors: PINHEIRO GOMES MARIA HELENA , BERNARDO DENIZE , BATISTA SILVEIRA BÁRBARA 
Published: June 2020

Este artigo de revisão da literatura especializada tem como objetivo trazer a discussão das práticas da psicologia da saúde dentro da Unidade Básica de Saúde (UBS). Para tanto, foi realizada uma pesquisa, através da busca ativa de informações em livros, revistas e artigos acadêmicos. A partir do levantamento da literatura revisada, evidenciamos a grande necessidade da atuação do (a) psicólogo (a) na Unidade Básica de Saúde. A psicologia na UBS é voltada para o acolhimento e a escuta terapêutica, e tem como proposta oferecer uma importante contribuição na abordagem contextualizada e integral dos indivíduos, familiares e toda a comunidade assistida. Diferenciando-se de uma prática curativa e individualizante, as ações estão voltadas para a promoção da autonomia, conscientização e empoderamento, visando à transformação social e melhora na qualidade de vida dentro do processo saúde-doença.
Palavras-chave: Psicólogo. Psicologia da Saúde. Unidade Básica da Saúde.
Coagulação intravascular disseminada e covid-19: mecanismos fisiopatológicos
Authors: Orsini Marco Antônio , Stephanie Fernandes do Nascimento Jacqueline , Santos Moraes Nunes Nicolle , Fernandes do Nascimento Janie Kelly , Azizi Marco , Cardoso Carlos Eduardo , Alves Pereira Tassiane Maria 
Published: June 2020

Introdução: A coagulação intravascular disseminada (CIVD) é, atualmente, definida como uma síndrome adquirida, caracterizada pela ativação difusa da coagulação intravascular, levando à formação e deposição de fibrina na microvasculatura. Há relatos de coagulopatia relacionada à Covid-19, doença pelo novo coronavírus SARS-CoV-2. Método: Atualização da Literatura acerca da associação entre Covid-19 e a presença de CIVD nas bases dados LIlacs, Bireme, Pubmed (inglês e português) no presente ano.Discussão: Alguns autores vêm estudando o papel da anticoagulação no manejo dos pacientes com infecção grave e (CIVD). Dados de inúmeras pesquisas sugerem efeitos benéficos do uso de anticoagulantes, o que vem sendo recomendado em algumas instituições no Brasil e no mundo. Vale ressaltar que a eficácia da medida ainda não foi validada. Conclusão: Até o presente momento não existem recomendações precisas em relação ao manejo dos pacientes com coagulopatia por Covid-19. Além disso, são excassos os dados na literatura que associam o uso de anticoagulantes ou antiplaquetários à ocorrência ou gravidade da Covid-19.
Modeling and Research of Resistance Characteristics of an Interlocking Side Milling Cutter with a Constructive Radial Feed When Processing Extended RK-profile Shafts
Authors: Kuts V. V. , Ponomaryov V. V. 
Published: June 2020

Purpose of research. The study is devoted to improving the resistance characteristics of an interlocking side milling cutter with a constructive radial feed when processing extended RK profile shafts while maintaining the required quality of surface treatment.Methods. Forming of RK profiles by milling with a disk cutter with a constructive radial feed allows you to obtain the required surface quality with high processing productivity. Due to the fact that the processing conditions for each cutting insert are different, it is necessary to study the resistance of interchangeable polyhedral inserts (IPI) and to search for structural solutions to reduce the range of change in resistance, while maintaining the quality of surface treatment of the PK profile. The influence of the use of an uneven pitch of location both on the quality of processing and on the resistance of each individual insert is studied.Results. The paper presents a method for calculating the resistance of an IPI of a interlocking side milling cutter during processing of an extended RK profile of a shaft, taking into account the individual conditions of their operation, namely: cutting speed, feed to the tooth, milling width and the diametrical position of the IPIConclusion. The analysis of the influence of the structural parameters of the cutter on changing the range of values of the resistance of IPI is made, and recommendations for its reduction are proposed. The main design parameters that affect the change in the resistance range are also identified.
Chip Breaking During Automated Processing by Cutting Hard-to-Work Steels by Integrated Use of Chip Breaking Tools and High-Temperature Embrittlement
Authors: Artamonov E. V. , Vasil'yev D. V. , Chernyshov M. O. 
Published: June 2020

Purpose of research is to increase productivity when cutting hard-to-work steels by integrated use of chip breaking tool geometry and cutting modes, which reduce the viscous properties of the chips and the processed material. The article discusses the problems of mechanical processing of parts of oil production equipment at enterprises in Western Siberia. It is presented the main condition for ensuring chip crushing and revealed the problem of low efficiency of existing methods in modern industry, to one degree or another guiding, curling and chip-cutting method.Methods. Simulation modeling, calculation of chip direction and crushing were performed; effective methods for solving the problem of chip crushing during the machining of parts made of materials with corrosion-resistant, heat-resistant properties were identified; laboratory, persistent tests of the complex method were carried out.Results of studying the mechanism of chip formation during processing by cutting heat-resistant and corrosionresistant alloys are given here. To solve the problem, we have formulated two tasks that were solved using information taken from the theory of material resistance, tensile diagrams when testing samples of corrosionresistant, heat-resistant materials with high operational properties. A chip breaking exchangeable insert with a variable rake angle is designed and presented. The results and analysis of laboratory test data, in which a plate with chip breaking geometry of the front surface is used, are presented, the operating conditions of effective chip breaking are shown. The conclusions on the solution of the first problem are formulated, it is shown that the determining factor in chip breaking is the physical and mechanical characteristics of the processed material, which vary with different cutting conditions.Conclusion. The work shows that chip removal from the cutting zone prevents it from entering the cutting edge area, reducing impact loads on it and improving the quality of machining. It is possible to form stress concentrators in the chips, leading to chip self-destruction even when processing viscous heat-resistant alloys. The hypothesis is used that the temperature conditions for the maximum workability of heat-resistant and corrosion-resistant steels and alloys correspond to the conditions of high-temperature embrittlement, with the help of which it is possible to create conditions for effective chip crushing and conditions for maximum workability when cutting heat-resistant steels and alloys.