bluuebunny/crossref_metadata_2025_split huggingface dataset:
ZhenQi FuZheng Formula Improves Mouse Hematopoietic Function after Cyclophosphamide-Induced Damage via Enhancing Macrophage Colony-Stimulating Factor Concentrations
Authors: Chu Qiubo , Dou Bo , Wang Shimiao , Li Lanzhou , He Jiawei , Wang Xue , Lee Robert J. , Li Dongjie , Wang Di 
Published: June 2020

Abstract
Background: Among the hematopoietic system, bone marrow has been recognized as the major source of hematopoietic progenitor cells (HPCs). Immuosuppression can inhibit the growth of bone marrow cells and cyclophosphamide (CTX) has been reported to induce myelosuppression. Regeneration of the immune system and hematopoietic function has become a primary problem in tumor adjuvant therapy. The present study investigated the effects of ZhenQi FuZheng formula (ZQFZ) on hematopoietic function in cells and murine models of cyclophosphamide(CTX)-induced immunosuppression and hematopoietic dysfunction. Methods: According to antibody chip analysis and enzyme-linked immunosorbent assay, ZQFZ regulated seven cytokines in serum and the spleen. Results: In Nrf2-siRNA transfected K562 cells, the regulatory effects of ZQFZ on the expression concentrations of macrophage colony-stimulating factor (M-CSF) and hematopoietic related proteins were strongly abolished. In immunosuppressed mice, ZQFZ enhanced the NK cell activities and regulated the concentrations of cytokines related to immune function. ZQFZ induced proliferation and differentiation, and upregulated the expression of hematopoietic-related proteins, including p90 ribosomal S6 kinases 1 (RSK1p90), c-Myc, and ETS transcription factor in CHRF and/or K562 cells. In mice with hematopoietic dysfunction, ZQFZ contributed to the recovery of blood cell concentrations in peripheral blood to normal values and enhanced the percentages of B lymphocytes (LYs) and juvenile cells in the bone marrow.Conclusions: In spleens of hematopoiesis damaged mice and primary cultured bone marrow cells, ZQFZ upregulated the expression concentrations of NF-E2-related Factor 2 (Nrf2) and its downstream proteins, and the activation of nuclear factor kappa-B. ZQFZ showed remarkably beneficial effects on the bone marrow hematopoietic system, at least partially, by enhancing the concentrations of M-CSF.
Tissue-nonspecific Alkaline Phosphatase Blockade Ameliorates Behavioural Deficits and Life-expectancy on Tauopathies
Authors: Sebastián-Serrano Álvaro , Merchán-Rubira Jesús , Di-Lauro Caterina , Bianchi Carolina , Narisawa Sonoko , Millan Jose Luis , Avila Jesus , Hernandez Felix , Diaz-hernandez Miguel 
Published: June 2020

Abstract
The authors have removed this preprint from Research Square.
Research on Personal Credit Evaluation of Internet Finance Based on Blockchain and Decision Tree Algorithm
Authors: Zhao Yuhao 
Published: June 2020

Abstract
With the development of Internet finance, existing financial platforms have gradually formed a large-scale, dynamic operating environment. How to ensure information security and realize personal credit evaluation is an urgent problem to be solved in the development of Internet financial platforms. The rise of blockchain technology has provided new solutions for the management of Internet financial platforms and information security. In view of the shortcomings of the current Internet financial credit evaluation, this article discusses the key standards of personal credit evaluation. With the help of blockchain, decision tree and other technologies, this paper designs the credit evaluation process and establishes personal credit evaluation technology. Experiments and analysis show that this technology can effectively improve the transparency of personal credit information in Internet finance. This technology is used to study credit risk assessment factors and provide new solutions for the intelligent transformation and upgrading of Internet finance.
Clinical characteristics of 50 children with azygos lobe: a retrospective study
Authors: Wang Lili , Zhao Fumin , Liu Hanmin , Chen Lina 
Published: June 2020

Abstract
Background
An azygos lobe is a rare anatomic variant of the lung which may be misdiagnosed as other pathological conditions. This study aimed to summarize the clinical characteristics and imaging manifestations of azygos lobe in children, which will be helpful for reasonable management and counseling of their families. Children with imaging findings of azygos lobe from West China Second University Hospital of Sichuan University (Chengdu, China) between November 2008 and February 2019 were enrolled in the study. Clinical and radiologic characteristics of these patients were collected and analyzed.
Results
In total, 50 patients were included. 28% children were diagnosed as azygos lobe incidentally. The other 72% cases were diagnosed when they manifested respiratory symptoms including cough (18/50, 36%), fever (17/50, 34%), wheezing (9/50, 18%), dyspnea (7/50, 14%), and cyanosis (4/50, 8%). Congenital heart disease, Down syndrome, and other respiratory malformations were also found in a small proportion of these patients. In our study, all cases with azygos lobe had the right lung involved. Other imaging manifestations of these cases consisted of increased marking and infiltrates in the lung fields, local emphysema, consolidation, and atelectasis et al. Among the patients, only 8 patients (16%) diagnosed as azygos lobe infection. Compared with the non-infected group,there were no valuable risk factors related to the azygos lobe infection. And all the children with azygos lobe infection were cured after reasonable anti-infective treatment.
Conclusion
Children with azygos lobe were diagnosed mostly because of respiratory symptoms and these children had a good prognosis after resonable treatment. Only a small number of patients suffered from azygos lobe infection. Some of the patients with azygos lobe were found to have other organ malformations as well, which needed further monitoring and study.
Measles Outbreak in Complex Emergency: Estimating Vaccine Effectiveness and Evaluation of the Vaccination Response in Borno State, Nigeria, 2019
Authors: Baptiste Anne Eudes Jean , Wagai John , Luce Richard Ray , Masresha Balcha Girma , Klinkenberg Don , Veldhuijzen Irene , Oteri Joseph , Dieng Boubacar , Ogbodo Caroline , Mele Sule , Audu Musa Idowu , Braka Fiona , Hahné Susan , Sanders Lieke E.A.M. , Hak Eelko 
Published: June 2020

Abstract
Background: From January to May 2019, large measles outbreaks affected all states in Nigeria and the Federal Capital Territory. Borno state was the most affected by the measles outbreak recording 15,237 suspected cases with the state capital of Maiduguri having 1,125 confirmed cases by March 2019. Twenty-two Local Government Areas (LGAs or Districts) and 37 internally displaced persons (IDPs) camps had been affected. In response to the situation, outbreak response immunization (ORI) was conducted. In addition to conventional vaccination teams, special teams were deployed in security compromised areas, areas with migrants, and for nomadic and IDPs. Here we describe the ORI and estimated the vaccine effectiveness (VE) for measles-containing vaccine (MCV) to assess the population-level impact. A post-campaign coverage survey (PCCS) was also carried out to assess the vaccination coverage.Methods: We reviewed the outbreak response-associated immunization activities, and conducted an analysis of the surveillance and the outbreak investigation reports. We evaluated VE of MCV by applying the screening-method using data from the measles outbreak and the latest mass vaccination campaign. The PCCS was conducted after completion of the ORI through a quantitative survey assessment in 1,800 households in 12 LGAs involved in the response.Results: Of the total 15,237 reported measles cases, 2,002 cases were line-listed and investigated, and 737 were confirmed for measles as at epidemiological week 9. Of the investigated cases 67.3% (n = 1,348) were between 9 and 59 months of age. Among the 737 confirmed cases, only 9% (n = 64) stated being vaccinated with at least 1 dose of MCV. The median VE for MCV is 96.7% (95%CI: 94.7 – 98.1), and 87.3% (95%CI: 71.0 – 95.2) when vaccine was received at 9 – 11. The aggregated weighted vaccination coverage is 85.7% (95%CI: 79.6 – 90.1).Conclusion: The experience in Borno demonstrates that adequate VE can be obtained in conflict-affected areas. In complex emergency affected by measles outbreaks, health authorities may consider integration with other health strategies and the engagement of security personnel as part of the ORI activities.
Effect of antifibrotic agents on survival in idiopathic pulmonary fibrosis patients according to forced vital capacity: a real-world retrospective cohort study in Japan
Authors: Saeki Sho , Nishiyama Osamu , Yamazaki Ryo , Yoshikawa Kazuya , Shirahase Ken , Gose Kyuya , Oomori Takashi , Nishikawa Yusaku , Sano Akiko , Sano Hiroyuki , Iwanaga Takashi , Tohda Yuji 
Published: June 2020

Abstract
Background

Antifibrotic agents suppress the decline in forced vital capacity (FVC) and disease progression in idiopathic pulmonary fibrosis (IPF) patients. However, their effect on survival prognosis and differences in this effect according to baseline lung function have been unexplored. Therefore, this study aimed to examine the effect of antifibrotics on survival prognosis and whether this effect differed according to baseline FVC.
Methods

Consecutive IPF patients from January 2008 to May 2019 were examined retrospectively. FVC and effect of pirfenidone or nintedanib therapy were assessed. FVC at registration was used to categorize the patients into mild: FVC % predicted ≥ 80%, moderate: FVC % predicted 50–80%, and severe: FVC % predicted < 50% IPF groups.
Results

In total, 172 IPF patients were included. The mean FVC % predicted was 77.4 ± 22.2%. The median survival periods of patients in the mild, moderate, and severe IPF groups were 1,452, 1,305, and 481 days, respectively. Significant differences were observed in survival between the mild and severe groups and the moderate and severe groups (p < 0.0001), but not between the mild and moderate groups (p = 0.20). The survival was longer in patients on antifibrotic therapy in the mild (p = 0.18) and moderate groups (p = 0.04), but not in the severe group (p = 0.93).
Conclusions

Antifibrotics extended the survival of IPF patients. The effect was obvious in patients with FVC % predicted of 50–80%, a tendency was observed in patients with FVC % predicted ≥ 80%, while no effect was observed in patients with FVC % predicted < 50%.
Persistent Hiccups as atypical presentation of COVID-19: a Case Report
Authors: Zahran Mohamed 
Published: June 2020

Abstract
The recent outbreak of SARS-CoV-2 has become pandemic since it began in late 2019. Typical symptoms include cough, shortness of breath or difficulty breathing, fever, myalgia and sore throat. There are other unusual or atypical presentations of COVID-19 in ORL practice. We report a 64-year-old male patient presenting with hiccups as the only symptom. Chest x-ray ray revealed new ground-glass opacities in both lung fields and he was found to be COVID-19 positive by RT-PCR. Early recognition of the COVID-19 atypical presentations by the Otolaryngologist facilitates subsequent management and case isolation to eliminate the risk of viral transmission.
Pharmacological evaluation of Salvadora persica on scopolamine-induced memory disorder
Authors: Biradar Prakash R. , Attar Vasim 
Published: June 2020

Abstract
Background: Salvadora persica root has been reported for various pharmacological activities and it can also be used for memory sharpening.Aim and Objective: The aim of the present study is to evaluate the effect of Salvadora persica root extract on scopolamine induced amnesia in ratsMaterials and Methods: The evaluation of Salvadora persica (S. persica) on scopolamine induced memory impairment was carried out using scopolamine 3mg/kg p.o for 7 days followed by treatment with Donepezil (3mg/kg) and S. persica root extract (250, 500, 1000 mg/kg) for 21 days; assessed by in vivo and in vitro studies. In vivo models Elevated Plus Maze (EPM), Morris Water Maze (MWM) and Passive Avoidance Paradigm (PA) was used for assessment of Transfer Latency (TL), Escape Latency (EL) and Step Through Latency (STL). In vitro studies on rat cerebral cortex and hippocampus AChE enzyme estimation, estimation of reduced glutathione, GSH and histopathological studies. Results: Treated animals shown increased STL whereas decreased TL and EL. In vitro studies of brain and parts of the brain showed decreased AChE enzyme activity in hippocampus and cerebral cortex, increased level of reduced glutathione level and reduced MDA levels. Considerable histopathological changes were seen in treated rat brain, specially in CA1 and CA3 neural intact in hippocampus region.Conclusion: Present study describes attenuation action of S. persica on memory impairment induced by scopolamine may be due to increase in Acetylcholine (ACh) level in brain by inhibiting AChE enzyme and antioxidant property of S. persica. Histopathological changes are seen in scopolamine treated rats and treatment rats.
Oral Microbiome Response to Powered vs Manual Toothbrush: Protocol for a Systematic Review &amp; Meta-Analysis
Authors: Riad Abanoub , Klugarova Jitka , Chuchmova Veronika , Slezakova Simona , Pokorna Andrea , Krsek Martin , Klugar Miloslav 
Published: June 2020

Abstract
Background: Subclinical changes in response to different types of toothbrushes represent a challenging knowledge gap in the context of self-administered oral hygiene regimes; therefore, this systematic review will be the first to evaluate the oral microbiome response to powered versus manual toothbrushes.Methods: We will conduct a systematic review using the Cochrane Handbook’s guidelines and will adhere to a standardized reporting format: Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). A comprehensive search strategy will be conducted in the following databases for published studies: Ovid MEDLINE(R), EMBASE, Cochrane Library, Web of Science, Scopus, ProQuest Central, ProQuest Dissertations Theses Global, Bibliographia Medica Cechoslovaca, and Dentistry Oral Sciences Source. Following a two-level screening process, data including the full reference, objectives, target population, description of the intervention and control intervention, outcome measures, design, length of the post-intervention follow-up period, and the study results will be extracted, synthesized, and reported. Risk of bias and quality of the studies will also be assessed.Discussion: No primary data collection will be undertaken; therefore, no formal ethical assessment is required. The results of this systematic review and meta-analysis will be published in a peer-reviewed journal.Registration: The protocol has been registered with the PROSPERO International Prospective Register of Systematic Reviews (CRD42020153557) since April 28th, 2020.
Clinicopathological and Prognostic Significance of Maspin Expression in Resected Non-Small Cell Lung Cancer: A Meta-Analysis
Authors: Wang Yan , Wang Yi , Dong Yingxian , Li Jialong , Che Guowei 
Published: June 2020

Abstract
Objective To explore the association of maspin expression with clinical pathological parameters and prognosis in non-small cell lung cancer (NSCLC) who received the surgical therapy.Methods The EMBASE, Web of Science and PubMed were searched to identify eligible studies to 3 December, 2019. The correlation of maspin expression with clinicopathological characteristics and survival of resected NSCLC patients was assessed by the combined relative risk (RR) and hazard ratio (HR) with corresponding 95% confidence interval (CI), respectively. All statistical analyses were performed via the Stata 12.0 software.Results A total of 12 articles involving 1771 patients were included. The results manifested that maspin was more prevalent in lung squamous cell carcinoma (SCC) (RR=0.36, 95% CI: 0.22-0.60, P＜0.001) and significantly associated with P53 expression (RR=1.50, 95% CI: 1.00-2.24, P=0.048), while no significant correlation of maspin with other clinicopathological parameters such as the gender, age, tumor size, lymph node metastasis, tumor node metastasis (TNM) stage or differentiation status was observed. As for the prognosis representing as overall survival (OS), disease-free survival (DFS) and cancer-specific survival (CSS), only significant association between maspin and CSS (HR=1.58, 95% CI: 1.16-2.15, P=0.003) was revealed. However, the subgroup analysis for OS based on the histology demonstrated that maspin expression was an unfavourable and favourable prognostic indicator in lung adenocarcinoma (HR=3.36, 95% CI: 1.44-7.87, P=0.005) and SCC (HR=0.44, 95% CI: 0.27-0.71, P=0.001), respectively.Conclusion Maspin expression was correlated with histology type and P53 expression, but no certain association of maspin with other clinicopathological characteristics or prognosis was observed in resected NSCLC. More well-designed prospective researches with big samples are still needed to further assess the clinicopathological and prognostic significance of maspin in NSCLC patients undergoing surgical resection.
Mesenchymal Stem Cells Regulate Activation of Microglia Cells to Improve Hippocampal Nerve Damage of Heat Stroke Rats
Authors: Wang Lu , Zhao Yan , Deng Zihui , Yuan Rui , Yang Mengmeng , Zhang Yu , Li Yun , Zhou Feihu , Kang Hongjun 
Published: June 2020

Abstract
Background: Heat stroke (HS) is a severe systemic inflammatory response disease caused by high fever, mainly with nervous system damage. Currently, mesenchymal stem cells (MSCs) have inflammation and immunomodulatory effects. Therefore, we aimed to explore the protective effect and mechanism of MSCs on HS-induced excessive inflammation and neurological dysfunction.Methods: A heat stroke Sprague-Dawley (SD) rat model was established at continuous high temperature (42 °C) and high humidity (70%-80%). After modeling, the rats were randomly divided into a heat stroke model group (HS group), a MSCs treatment group (HS+MSCs group), and a Control group in which the rats were kept at room temperature without any treatment. Survival analysis, neurological deficit scoring, pathological staining of hippocampus and cerebellum, immunofluorescence staining of microglia cells, and tissue level detection of inflammatory cytokines were performed in the three groups on day 1, 3, 7, 14 and 28 separately.Results: After heat stroke modeling, the rats were severely paralyzed and had a high mortality. MSCs treatment significantly reduced the mortality in both early stage (day 3) and late stage (day 28). MSCs treatment also significantly reduced the neurological impairment of heat stroke rats, and improved hippocampal and cerebellar pathology and neuronal cell damage. In addition, MSCs treatment significantly inhibited the overactivation of microglia in the hippocampus of HS rats as well as the levels of pro-inflammatory factors and chemokines in the hippocampus. In the early stage (day 1) of MSCs treatment, the activation of cerebellar microglia in the heat stroke rats was significantly promoted. Meanwhile, MSCs treatment had no significant inhibitory effect on the levels of pro-inflammatory factors in the cerebellar tissues of heat stroke rats, but can inhibit the levels of chemokines in the early stage.Conclusions: The application of MSCs for heat stroke treatment in rats can significantly reduce the mortality and neurological defects and improve the hippocampal injury. Meanwhile, MSCs can inhibit the over-activation of microglia cells in the hippocampus of heat stroke rats, which may be a mechanism of MSCs in protecting heat-stroke-caused hippocampal injury.
LGR4 Maintains HGSOC Cell Epithelial Phenotype and Stem-Like Traits
Authors: Wang Zhuo , Yin Ping , Sun Yu , Na Lei , Gao Jian , Wang Wei , Zhao Chenghai 
Published: June 2020

Abstract
Background: High-grade serous ovarian cancer (HGSOC) is lethal mainly due to extensive metastasis. Cancer cell stem-like properties are responsible for HGSOC metastasis. LGR4, a G-protein-coupled receptor, is involved in the maintenance of stem cell self-renewal and activity in some human organs. Methods: TCGA and CCLE database was interrogated for gene mRNA analysis in ovarian cancer tissues and cell lines. The interactions between LGR4 and ELF3 were validated through dual-luciferase reporter assays, Chip assays and Co-IP assays. Gain- and loss-of functions of LGR4, ELF3, FZD5 and WNT7B were performed to identify their roles in the behaviors of ovarian cancer cells. Flowcytometry analysis and tumorisphere formation assays were performed to identified their stem-like properties. In vivo experiments were performed as well.Results: LGR4 was shown to be overexpressed in HGSOCs and maintain the epithelial phenotype of HGSOC cells. LGR4 knockdown suppressed POU5F1, SOX2, PROM1 (CD133) and ALDH1A2 expression. Furthermore, LGR4 knockdown reduced CD133+ and ALDH+ subpopulations and impaired tumorisphere formation. To the contrary, LGR4 overexpression enhanced POU5F1 and SOX2 expression and tumorisphere formation capacity. LGR4 knockdown inhibited HGSOC cell growth and peritoneal seeding in xenograft models. Mechanistically, LGR4 and ELF3, an epithelium-specific transcription factor, formed a reciprocal regulatory loop, which was positively modulated by WNT7B/FZD5 pair. Consistently, knockdown of ELF3, WNT7B, and FZD5, respectively, disrupted HGSOC cell epithelial phenotype and stem-like properties. Conclusion: Together, these data demonstrate that WNT7B/FZD5-LGR4/ELF3 axis maintains HGSOC cell epithelial phenotype and stem-like traits; targeting this axis may prevent HGSOC metastasis.