bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contexto cultural y consejería escolar: sensibilidad cultural y defensa de la justicia social
Authors: Tatar Moshé 
Published: September 2020

En este estudio se destaca la importancia de la comprensión de los diferentes significados de la cultura para la práctica profesional del consejero psicológico escolar. Se sugieren dos aproximaciones hacia el concepto de cultura, en relación con la consejería psicológica: la primera señala la importancia de la cultura organizacional en la institución donde labora el consejero psicológico, mientras que la segunda —la aproximación multicultural— sugiere el complejo reconocimiento de la variedad de antecedentes étnico-culturales de aquellos que están comprometidos con la situación de consejería. Se analizan las prácticas profesionales según su relevancia para el refuerzo de las condiciones actuales, o como formas para promover el cambio. Los conceptos de empoderamiento y defensa para con los pacientes-clientes son señalados como los componentes principales en los nuevos roles que retan la profesión del consejero psicológico, sugiriéndose implicancias para la consejería psicológica.
Concepciones sobre la enseñanza de matemática en docentes de primaria de escuelas estatales
Authors: Moreano Giovanna , Asmad Ursula , Cruz Gustavo , Cuglievan Gisele 
Published: September 2020

Esta investigación forma parte de un estudio cualitativo, realizado en cinco escuelas estatales de Lima entre el 2005 y el 2007. El principal objetivo fue identificar las concepciones que los docentes sostenían sobre la enseñanza y aprendizaje de matemática para comprender su práctica pedagógica. La muestra estuvo conformada por nueve docentes de sexto grado de primaria. Los resultados muestran un fuerte arraigo de las concepciones pedagógicas tradicionales, que se encuentra reflejado en las prácticas pedagógicas de los docentes. La enseñanza de matemática se caracterizó por el seguimiento de reglas y procedimientos, la práctica rutinaria de ejercicios, el uso de palabras clave y la falta de un contexto significativo para su aprendizaje.
Bilateral Atypical Femur Fractures Complicated by Carbon Fiber Cephalomedullary Nail Fracture
Authors: Goodloe J. Brett , Toner Sarah , Althoff Alyssa , Herzog Leah , Rothwell Stacey , Reid Kristoff 
Published: September 2020

Case:
A 69-year-old woman with a history of breast cancer, on long-term antiresorptive medications, was treated with carbon fiber cephalomedullary nails for bilateral atypical femur fractures. Her thigh pain was presumed to be from metastatic lesions and was treated with radiation without standard imaging. The index procedure was complicated by a left nail fracture. The patient underwent exchange cephalomedullary nail fixation with plate augmentation. At 1 year, the patient was asymptomatic with radiographs demonstrating healing.

Conclusion:
This case highlights the importance of appropriate imaging in all patients with thigh pain on antiresorptive therapy and features an uncommon complication of carbon fiber cephalomedullary nail fixation.
Analysis of Risk Factor for Anastomotic Leakage after Lower Rectal Cancer Resection, Including Drain Type: A Retrospective Single-Center Study
Authors: Kinugasa Tetsushi , Nagasu Sachiko , Murotani Kenta , Mizobe Tomoaki , Ochi Takafumi , Isobe Taro , Fujita Fumihiko , Akagi Yoshito 
Published: September 2020

Abstract
Background: We investigated the correlations between surgery-related factors and the incidence of anastomotic leakage after low anterior resection (LAR) for lower rectal cancer.Methods: A total of 630 patients underwent colorectal surgery between 2011 and 2014 in our department. Of these, 97 patients (15%) underwent LAR and were enrolled in this retrospective study. Temporary ileostomy was performed in each patient.Results: Anastomotic leakage occurred in 21 patients (21.7%). Univariate analysis showed a significant association between operative duration (p=0.005), transanal hand-sewn anastomosis (p=0.014), and operation procedure (p=0.019) and the occurrence of leakage. Multivariate regression reanalysis showed that underlying disease (p=0.044), transanal hand-sewn anastomosis (p=0.019) and drain type (p=0.025) were significantly associated with the occurrence of leakage. The propensity-score analysis showed that closed drainage were 6.3 times more likely to have anastomotic leakage than open drainage in relation to the amount of postoperative drainage (ml), according to the inverse probability of treatment-weighted analysis.Conclusions: Our results indicate that underlying disease, transanal hand-sewn anastomosis, and closed drain may be a risk and predictive factors for anastomotic leakage after LAR for lower rectal cancer. The notable finding was that closed drainage was related to the occurrence of anastomotic leakage and closed drainage was correlated with less volume of postoperative drain discharge than open drain.
Evaluation of the contribution of tropical cyclone seeds to changes in tropical cyclone frequency due to global warming in high-resolution multi-model ensemble simulations
Authors: Yamada Yohei , Kodama Chihiro , Satoh Masaki , Sugi Masato , Roberts Malcolm J , Mizuta Ryo , Noda Akira T , Nasuno Tomoe , Nakano Masuo , Vidale Pier Luigi 
Published: September 2020

Abstract
Previous projections of the frequency of tropical cyclone genesis due to global warming, even in terms of sign of the change, depends on the chosen model simulation. Here, we systematically examine projected changes in tropical cyclones using six global atmospheric models with medium-to-high horizontal resolutions included in the sixth phase of the Coupled Model Intercomparison Project/High-Resolution Model Intercomparison Project. Changes in the frequency of tropical cyclone genesis could be broken down into the contributions from (i) the tropical cyclone seed, a depression having a closed contour of sea level pressure with a warm core and (ii) the survival rate, the ratio of the frequency of tropical cyclone genesis to that of tropical cyclone seeds. The multi-model ensemble mean indicates that tropical cyclone genesis frequencies are significantly decreased during the period 1990–2049, which is attributable to changes in tropical cyclone seeds. Analysis of the individual models shows that although most models project a more or less decreasing trend in tropical cyclone genesis frequencies and seeds, the survival rate also contributes to the result in some models. The present study indicates the usefulness of decomposition into the frequency of the tropical cyclone seeds and the survival rate to understand the cause of uncertainty in projected frequencies of tropical cyclone genesis.
Gene expression kinetics of Exaiptasia pallida innate immune response to Vibrio parahaemolyticus infection
Authors: Seneca Francois , Davtian David , Boyer Laurent , Czerucka Dorota 
Published: September 2020

Abstract
Background: Recent sequencing projects on early-diverging metazoans such as cnidarians, have unveiled a rich innate immunity gene repertoire; however, little is known about immunity gene regulation in the host’s early response against marine bacterial pathogens over time. Here, we used RNA-seq on the sea anemone Exaiptasia pallida (Ep) strain CC7 as a model to depict the innate immune response during the onset of infection with the marine pathogenic bacteria Vibrio parahaemolyticus (Vp) clinical strain O3:K6, and lipopolysaccharides (LPS) exposure. Pairwise and time series analyses identified the genes responsive to infection as well as the kinetics of innate immune genes over time. Comparisons between the responses to live Vp and purified LPS was then performed.Results: Gene expression and functional analyses detected hundreds to thousands of genes responsive to the Vp infection after 1, 3, 6 and 12 hours, including a few shared with the response to LPS. Our results bring to light the first indications that non-canonical cytoplasmic pattern recognition receptors (PRRs) such as NOD-like and RIG-I-like receptor homologs take part in the immune response of Ep. Over-expression of several members of the lectin-complement pathways in parallel with novel transmembrane and Ig containing ficolins (CniFLs) suggest an active defense against the pathogen. Although lacking typical Toll-like receptors (TLRs), Ep activates a TLR-like pathway including the up-regulation of MyD88, TRAF6, NF-κB and AP-1 genes, which are not induced under LPS treatment and therefore suggest an alternative ligand-to-PRR trigger. Two cytokine-dependent pathways involving Tumor necrosis factor receptors (TNFRs) and several other potential downstream signaling genes likely lead to inflammation and/or apoptosis. Finally, both the extrinsic and intrinsic apoptotic pathways were strongly supported by over-expression of effector and executioner genes.Conclusions: To our knowledge, this pioneering study is first to follow the kinetics of the innate immune response in a cnidarian during the onset of infection with a bacterial pathogen. Overall, our findings reveal the involvement of both novel immune gene candidates such as NLRs, RLRs and CniFLs, and previously identified TLR-like and apoptotic pathways in anthozoan innate immunity with a large amount of transcript-level evidence.
Brain SPECT perfusion and PET metabolism as discordant biomarkers in major depressive disorder
Authors: Tastevin Maud , Boyer Laurent , Korchia Theo , Fond Guillaume , Lançon Christophe , Richieri Raphaëlle , Guedj Eric 
Published: September 2020

Abstract
Background: Brain SPECT perfusion and PET metabolism have been, most often interchangeably, proposed to study the underlying pathological process in major depressive disorder (MDD). The objective of this study was to specify similarities and inconsistencies between these two biomarkers according to global characteristics of the disease. We conducted a retrospective study in 16 patients suffering from treatment-resistant MDD who underwent, during the same current episode, a cerebral perfusion SPECT with 99mTc-HMPAO and a metabolic PET with 18F-FDG. Whole-brain voxel-based SPM(T) maps were generated in correlation with the number of depressive episodes and in correlation with the depression duration, separately for the two exams (p-voxel<0.001 uncorrected, k>20). Results: No significant correlations were found between brain metabolism and either the number of depressive episodes or the duration of the disease, even at an uncorrected p-voxel<0.005. On the other hand, the increased number of depressive episodes was correlated with decreased perfusion of the right middle frontal cortex, the right anterior cingulum cortex, the right insula, the right medial temporal cortex and the left precuneus. The increased depression duration was correlated with decreased perfusion of the right anterior cingulum cortex. Conclusions: This preliminary study demonstrates more significant results with brain perfusion compared with glucose metabolism in treatment-resistant MDD, highlighting the value of brain SPECT despite less favourable instrumentation detection compared to PET.
Long-term results of additional pulmonary blood flow with bidirectional cavopulmonary shunt
Authors: Kowatari Ryosuke , Suzuki Yasuyuki , Daitoku Kazuyuki , Fukuda Ikuo 
Published: September 2020

Abstract
Objective: We evaluated additional pulmonary blood flow at the time of bidirectional cavopulmonary shunt and its effects on the Fontan procedure and long-term outcome of Fontan circulation and liver function.Methods: We included 22 patients (16 boys, 6 girls) having undergone bidirectional cavopulmonary shunt with additional pulmonary blood flow between April 2002 and January 2016. Mean age and body weight were 20 ± 13 months and 7.5 ± 6.5 kg, respectively. We retrospectively evaluated the patients' clinical data, including cardiac catheterization data, liver function, and liver fibrosis markers.Results: All patients were alive with a New York Heart Association status of I at the long-term follow-up. Changes between pre-bidirectional cavopulmonary shunt and 101 months after the Fontan procedure included the following: the cardiothoracic ratio of chest X-ray decreased from 52.2 ± 3.9% to 41.8 ± 5.9% (p < 0.001); systemic ventricle end-diastolic pressure decreased from 11.4 ± 3.2 mmHg to 6.9 ± 3.6 mmHg (p < 0.001); and the pulmonary artery index decreased from 485.1 ± 272.3 to 269.5 ± 100.5 (p = 0.02). Type IV collagen, hyaluronic acid, and procollagen levels increased over the normal range 116 months after the Fontan procedure.Conclusions: The additional pulmonary blood flow at the time of bidirectional cavopulmonary shunt may contribute to pulmonary arterial growth at the Fontan procedure with low pulmonary arterial resistance and without ventricle volume overload. The Fontan circulation was well-maintained at the long-term follow-up, while liver fibrosis markers were above their normal values.
Potential Biomarkers of Acute Myocardial Infarction Based on Co-Expression Network Analysis
Authors: Ding Zhiwen , Hu Zhaohui , Ding Xiangjun , Ji Yuyao , li Guiyuan , Wang Yiping , Xie Shengquan , Liu Xiaohong 
Published: September 2020

Abstract
Background: Acute myocardial infarction (AMI) is a common cause of death in many countries. Analyzing the potential biomarkers of AMI is crucial to understanding the molecular mechanism of disease. However, specific diagnostic biomarkers have not been fully elucidated, and candidate regulatory targets for AMI have not been determined.Methods: In this study, AMI gene chip data GSE48060, blood samples from normal cardiac function controls (n = 21) and AMI patients (n = 26) were downloaded from Gene Expression Omnibus. The differentially expressed genes (DEGs) of AMI and control group were identified with Online tool GEO2R. the genes co-expressed with were found. The co-expression network of DEGs was analyzed by calculating the Pearson correlation coefficient of all gene pairs, MR screening and cutoff threshold screening. Then, GO database was used to analyze the function and pathway enrichment of genes in the most important modules. KEGG DISEASE and BioCyc were used to analyze the hub gene in the module to determine important sub-pathways. In addition, the expression of hub genes were certified by RT-qPCR in AMI and control specimens.Results: This study identified 52 DEGs, including 26 up-regulated genes and 26 down-regulated genes. Co-expression network analysis of 52 DEGs revealed that there are mainly three up-regulated genes (AKR1C3, RPS24 and P2RY12) and three down-regulated genes (ACSL1, B3GNT5 and MGAM) as key hub genes in the co-expression network. Furthermore, GO enrichment analysis was performed on all AMI co-expression network genes and found to be functionally enriched mainly in RAGE receptor binding and negative regulation of T cell cytokine production. In addition, through KEGG DISEASE and BioCyc analysis, the functions of genes RPS24 and P2RY12 were enriched in cardiovascular diseases, AKR1C3 was enriched in cardenolide biosynthesis, MGAM was enriched in glycogenolysis, B3GNT5 was enriched in glycosphingolipids biosynthesis, and ACSL1 enriched in icosapentaenoate biosynthesis II. Conclusion: The hub genes AKR1C3, RPS24, P2RY12, ACSL1, B3GNT5 and MGAM are potential targets of AMI and have potential application value in the diagnosis of AMI.
Effects of Autonomic Nervous System Activation on Endothelial Function in Response to Acute Exercise in Hypertensive Individuals: Study Protocol for A Randomized Double-Blind Study
Authors: Waclawovsky Gustavo , Boll Liliana FC , Neto Salvador Gomes , Irigoyen Maria Cláudia , Lehnen Alexandre Machado 
Published: September 2020

Abstract
Background: Arterial hypertension has a direct association with endothelial dysfunction and major cardiovascular events. There is evidence showing the benefits of aerobic exercise on flow-mediated dilation (FMD) in hypertensive individuals but little is known about the effect of autonomic nervous system (ANS) activation on FMD of the brachial artery in response to different types of exercise in this specific population. This study aims to examine the effects of ANS activation on FMD of the brachial artery in response to exercise in hypertensive individuals following a session of different types of exercise including aerobic exercise (AE), resistance exercise (RE) or combined exercise (CE). Methods: Thirty-nine hypertensive volunteers aged 35 to 55 years will be randomly assigned to two exercise sessions: AE (40 minutes on a cycle ergometer at 60% of HR reserve); RE (4 lower limb sets with 12 repetitions at 60% 1-RM for 40 minutes) or CE (RE for 20 minutes + AE for 20 minutes). Each exercise group will be randomized to receive either an α1-adrenergic blocker (doxazosin 0.05 mg/kg-1) or placebo. Ultrasound measurement of FMD is performed 10 minutes before and 10, 40 and 70 minutes after exercise. ANS activation is monitored using a Finometer and measurements are taken during 10 minutes before each FMD assessment. Arterial stiffness is assessed by pulse wave velocity (PWV) analysis using a Complior device. Discussion: We expect to demonstrate the effect of ANS activation on FMD of the brachial artery in hypertensive individuals in response to different types of exercise. This study may give some insight on how to improve exercise prescription for hypertension management. Trial registration: www.clinicaltrials.gov (ID: NCT04371757).
Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature
Authors: Arshad Muhammad , Puri Alpa , Simkovich Aaron J , Renaud Justin , Gruber Margaret Y , Marsolais Frédéric , Hannoufa Abdelali 
Published: September 2020

Abstract
Background: Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level. Results: In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 ° C) for 24 hours. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log 2 FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors. Conclusions: These results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.
Knowledge, attitudes, and practices of tertiary education students in regard to air pollution in Windhoek, Namibia: a cross sectional study
Authors: KEMBA HAUSHIKU , Tshifhiwa Nkwenika , Vusumuzi Nkosi 
Published: September 2020

Abstract
Background: Knowing the residents' knowledge, attitude, and practices to air pollution is necessary for developing interventions specific to that population. Air pollution remains a neglected environmental concern in Windhoek, Namibia and people’s perceptions about air pollution and health have not been documented. This study investigated the knowledge, attitudes and practices of tertiary students to air pollution in Windhoek. Methods: Students from five tertiary institutions in Windhoek were included in cross sectional study. A previously validated questionnaire was used to collect data from the study participants. Frequency and a corresponding percentage were reported for categorical data, whereas the association between knowledge of air pollution and perceptions was evaluated using the Chi-square test. Kruskal Wallis rank test was used to assess the statistical difference in knowledge, attitudes and practices. Data analysis was performed using STATA 15 and evaluated at a 5% level of significance Results: More than half (59.4%) of the respondents thought that air pollution was a major problem, while over 90% knew that air pollution could negatively impact health. The media was found to be the main source of information for respondents (91.1%). Despite having the knowledge, less than a third (27%) of the respondents regularly took action out of concern of air pollution. There was a statistically significant association between knowledge, attitudes, and practices to air pollution (all P < 0.05). Conclusions: Air pollution interventions targeting tertiary students should be aimed at informing them on ways in which they could contribute individually and collectively to reducing air pollution and its negative effects.
Vibrionaceae core, shell and cloud genes are non-randomly distributed on Chr 1: An hypothesis that links the genomic location of genes with their intracellular placement
Authors: Sonnenberg Cecilie Bækkedal , Kahlke Tim , Haugen Peik 
Published: September 2020

Abstract
Background: The genome of Vibrionaceae bacteria, which consists of two circular chromosomes, is replicated in a highly ordered fashion. In fast-growing bacteria, multifork replication results in higher gene copy numbers and increased expression of genes located close to the origin of replication of Chr 1 (ori1). This is believed to be a growth optimization strategy to satisfy the high demand of essential growth factors during fast growth. The relationship between ori1-proximate growth-related genes and gene expression during fast growth has been investigated by many researchers. However, it remains unclear which other gene categories that are present close to ori1 and if expression of all ori1-proximate genes is increased during fast growth, or if expression is selectively elevated for certain gene categories.Results: We calculated the pangenome of all complete genomes from the Vibrionaceae family and mapped the four pangene categories, core, softcore, shell and cloud, to their chromosomal positions. This revealed that core and softcore genes were found heavily biased towards ori1, while shell genes were overrepresented at the opposite part of Chr 1 (i.e., close to ter1). RNA-seq of Aliivibrio salmonicida and Vibrio natriegens showed global gene expression patterns that consistently correlated with chromosomal distance to ori1. Despite a biased gene distribution pattern, all pangene categories contributed to a skewed expression pattern at fast-growing conditions, whereas at slow-growing conditions, softcore, shell and cloud genes were responsible for elevated expression.Conclusion: The pangene categories were non-randomly organized on Chr 1, with an overrepresentation of core and softcore genes around ori1, and overrepresentation of shell and cloud genes around ter1. Furthermore, we mapped our gene distribution data on to the intracellular positioning of chromatin described for V. cholerae, and found that core/softcore and shell/cloud genes appear enriched at two spatially separated intracellular regions. Based on these observations, we hypothesize that there is a link between the genomic location of genes and their cellular placement.