bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postsynthesis of zwitterionic hydrophilic composites for enhanced enrichment of N‐linked glycopeptides from human serum
Authors: Liu Bin , Wang Baichun , Yan Yinghua , Tang Keqi , Ding Chuan‐Fan 
Published: October 2019

RationaleGlycosylation of proteins plays an important role in life activities, but the concentration of naturally occurring glycopeptides is usually relatively low, and glycosylation has microfacies heterogeneity, so direct mass spectrometry is not feasible. Therefore, selective enrichment of glycopeptides before mass spectrometry has turned into an urgent problem to be resolved.MethodsHerein, the zwitterionic L‐cysteine functionalized hydrophilic graphene oxide composite (GO@PDA@MIL‐125‐NH2@Au@L‐Cys) was prepared via a postsynthetic method. The obtained material was used for glycopeptide enrichment. The enriched peptides were then detected using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) to demonstrate the enrichment performance of the material.ResultsIn the actual enrichment process, GO@PDA@MIL‐125‐NH2@Au@L‐Cys nanomaterials exhibited high selectivity (1:1000), outstanding sensitivity (0.5 fmol), and excellent repeatability for the enrichment of glycopeptides. In addition, the proposed material showed good performance in the enrichment of glycopeptides from complex biosamples; 56 glycopeptides were detected from 2 μL of human serum using MALDI‐TOFMS.ConclusionsThe experimental results showed that GO@PDA@MIL‐125‐NH2@Au@L‐Cys exhibited excellent performance on glycopeptide analysis. It has great potential in the enrichment of glycopeptides and provides new ideas for synthetic materials with better enrichment properties in the future.
Separation and detection of minimal length glycopeptide neoantigen epitopes centering the GSTA region of MUC1 by liquid chromatography/mass spectrometry
Authors: Zhou Dapeng , Xiao Kaijie , Tian Zhixin 
Published: October 2019

RationaleWe previously isolated antibodies binding to glycopeptide neoantigen epitopes centering the GSTA sequence of the highly glycosylated tandem repeat region of MUC1. Epitopes centering the GSTA sequence are also predicted by NetMHC programs to bind to MHC molecules. Detecting MUC1 glycopeptide epitopes remains a challenge since antigenic epitopes are often shorter than 10 amino acids.MethodsIn this study, we used pronase from Streptomyces griseus, which has no amino acid sequence preference for enzymatic cleavage sites, to digest synthetic glycopeptides RPAPGST (Tn)APPAHG and RPAPGS (Tn)TAPPAHG, and analyzed the digests by liquid chromatography/mass spectrometry (LC/MS) using electron transfer dissociation (ETD) and higher‐energy collisional dissociation (HCD) methods with an Orbitrap Fusion Lumos Tribid mass spectrometer.ResultsWe found that short glycopeptides containing 8 to 11 amino acids could be efficiently generated by pronase digestion. Such glycopeptides of minimal epitope lengths were clearly distinguished by characteristic MS/MS ion patterns and LC elution profiles. A glycopeptide library was generated which may serve as a standard for measuring neoantigen epitopes centering the GSTA sequence.ConclusionsOur data established the LC/MS/MS identities of a clinically relevant MUC1 glycopeptide neoantigen epitope centering the GSTA motif. A library of short MUC1 glycopeptides centered on the GSTA motif was created, which is a critical step for analysis of such antigen epitopes in real biological samples.
Effects of organic removal techniques prior to carbonate stable isotope analysis of lacustrine marls: A case study from palaeo‐lake Fucino (central Italy)
Authors: Mannella Giorgio , Zanchetta Giovanni , Regattieri Eleonora , Perchiazzi Natale , Drysdale Russell N. , Giaccio Biagio , Leng Melanie J. , Wagner Bernd 
Published: October 2019

RationaleThe suitability of organic matter (OM) removal pre‐treatments in isotopic studies of lacustrine carbonates is currently under debate. Naturally occurring OM seems to have a negligible effect on the bulk isotopic composition of carbonates compared with changes induced by pre‐treatments. This study provides further insights into the possible effects induced by commonly used pre‐treatments on natural lacustrine carbonates.MethodsSixteen samples from the Fucino F1–F3 lacustrine succession (Abruzzo, central Italy) were characterised for their mineralogical and geochemical composition and each was split into three identical aliquots. One aliquot was left untreated while the remaining two were treated with NaOCl and H2O2 dilutions. The same treatment was applied to an internal standard consisting of pure Carrara marble. The treated and untreated samples were analysed for their carbon (δ13C values) and oxygen (δ18O values) isotope compositions using an Analytical Precision AP2003 isotope ratio mass spectrometer.ResultsThe samples had variable proportions of endogenic and detrital components, the detrital portion being more (less) abundant during colder (warmer) climate phases. We observed that neither the NaOCl nor the H2O2 treatment was able to completely remove OM and therefore there was selective removal of compounds within the OM pool. A possible effect of pre‐treatment is the loss of carbonates intimately interspersed within the OM, as suggested by the evolution of isotopic ratios towards the local detrital array.ConclusionsOur study highlights sample‐specific changes in geochemistry associated with sample pre‐treatments; however, such changes do not seem to lead to either systematic and/or predictable isotopic shifts. We suggest that the suitability of NaOCl or H2O2 pre‐treatments for OM removal should be evaluated on a case‐by‐case basis. In the specific case of lacustrine marls from palaeo‐lake Fucino containing relatively low amounts of OM and in which both detrital and endogenic carbonates occur, both pre‐treatments should be avoided.
A predictive spatial model for roasted coffee using oxygen isotopes of α‐cellulose
Authors: Driscoll Avery W. , Howa John D. , Bitter Nicholas Q. , Ehleringer James R. 
Published: October 2019

RationaleFraudulent region‐of‐origin labeling is a concern for high‐value, globally traded commodities such as coffee. The oxygen isotope ratio of cellulose is a useful geographic tracer, as it integrates climate and source water signals. A predictive spatial model (“isoscape”) of the δ18O values of coffee bean cellulose is generated to evaluate coffee region‐of‐origin claims.MethodsThe oxygen isotope ratio of α‐cellulose extracted from roasted coffee beans was measured via high‐temperature conversion elemental analyzer/isotope ratio mass spectrometry (TC‐EA/IRMS) and used to calculate the δ18O value of coffee bean water. The 18O enrichment of coffee bean water relative to the δ18O value of local precipitation was modeled as a function of local temperature and humidity. This function was incorporated into a mechanistic model of cellulose δ18O values to predict the δ18O values of coffee bean cellulose across coffee‐producing regions globally.ResultsThe δ18O values of analyzed coffee bean cellulose ranged from approximately +22‰ to +42‰ (V‐SMOW). As expected, coffees grown in the same region tended to have similar isotope ratios, and the δ18O value of coffee bean cellulose was generally higher than the δ18O value of modeled stem cellulose for the region. Modeled δ18O values of coffee cellulose were within ±2.3‰ of the measured δ18O value of coffee cellulose.ConclusionsThe oxygen isotope ratio of coffee bean cellulose is a useful indicator of region‐of‐origin and varies predictably in response to climatic factors and precipitation isotope ratios. The isoscape of coffee bean cellulose δ18O values from this study provides a quantitative tool that can be applied to region‐of‐origin verification of roasted coffee at the point‐of‐sale.
Hybrid dynamical systems with hybrid inputs: Definition of solutions and applications to interconnections
Authors: Bernard Pauline , Sanfelice Ricardo G. 
Published: October 2019

SummaryIn this paper, we define solutions for hybrid systems with prespecified hybrid inputs. Unlike previous work where solutions and inputs are assumed to be defined on the same domain a priori, we consider the case where intervals of flow and jump times of the input are not necessarily synchronized with those of the state trajectory. This happens in particular when the input is the output of another hybrid system, for instance, in the context of observer design or reference tracking. The proposed approach relies on reparametrizing the jumps of the input in order to write it on a common domain. The solutions then consist of a pair made of the state trajectory and the reparametrized input. Our definition generalizes the notions of solutions of continuous‐time and discrete‐time systems with inputs. We provide an algorithm that automatically performs the construction of solutions for a given hybrid input. In the context of hybrid interconnections, we show how the solutions of the individual systems can be linked to the solutions of a closed‐loop system. Example illustrate the notions and the proposed algorithm.
Global finite‐time stabilization for a class of high‐order switched nonlinear systems via sampled‐data control
Authors: Jiang Yan , Zhai Junyong 
Published: October 2019

SummaryThis paper investigates the global finite‐time stabilization for a class of high‐order switched nonlinear systems via the sampled‐data output feedback control. Firstly, we design a continuous‐time output feedback controller for the nominal part via adding a power integrator technique. Based on the homogeneous theory, together with the Gronwall‐Bellman inequality, a sampled‐data output feedback controller is designed with suitable sampling periods to ensure that the closed‐loop system can be globally stabilized in finite time. In the meantime, the proposed control method can be extended to the switched nonlinear systems with an upper‐triangular growth condition. Finally, two examples are presented to illustrate the validity of the proposed control scheme.
Multidimensional Taylor network adaptive control for MIMO time‐varying uncertain nonlinear systems with noises
Authors: Zhang Chao , Yan Hong‐Sen 
Published: October 2019

SummaryIn this paper, an adaptive control approach based on the multidimensional Taylor network (MTN) is proposed here for the real‐time tracking control of multiple‐input–multiple‐output (MIMO) time‐varying uncertain nonlinear systems with noises. Two MTNs are used to formulate the optimum control and adaptive filtering approaches. The feed‐forward MTN controller (MTNC) is developed to realize the precise tracking control. The closed‐loop errors between the filtered outputs and expected values are directly chosen as the MTNC's inputs. A valid initial value selection scheme for the weights of the MTNC, which can ensure the initial stability of adaptive process, is introduced. The proposed MTNC can update its weights online according to errors caused by system's uncertain factors, based on stable learning rate. The resilient backpropagation algorithm and the adaptive variable step size algorithm via linear reinforcement are utilized to update the MTNC's weights. The MTN filter (MTNF) is developed to eliminate measurement noises and other stochastic factors. The proposed adaptive MTN filtering system possesses the distinctive properties of the Lyapunov theory–based adaptive filtering system and MTN. Lyapunov function of the filtering errors between the measured values and MTNF's outputs is defined. By properly choosing the weights update law in the Lyapunov sense, the MTNF's outputs can asymptotically converge to the desired signals. The design is independent of the stochastic properties of the input disturbances. Simulation of the MTN‐based control is conducted to test the effectiveness of the presented results.
Supramolecular Protein Nanodrugs with Coordination‐ and Heating‐Enhanced Photothermal Effects for Antitumor Therapy
Authors: Sun Haifeng , Chang Rui , Zou Qianli , Xing Ruirui , Qi Wei , Yan Xuehai 
Published: October 2019

AbstractSupramolecular protein nanodrugs provide opportunities for improving antitumor therapeutic efficiency and lowering toxicity. However, protein nanodrugs that have robust structural stability and enhanced therapeutic efficiency are still in infancy. In this study, photothermal protein nanodrugs are constructed through a supramolecular approach along with heating by using proteins, photosensitizers, and metal ions as the building blocks. The metal coordination and heating improve not only the structural stability but also photothermal performance of the resulting nanodrugs. By virtue of the first integration of coordination‐ and heating‐enhanced photothermal effects, the nanodrugs show superior photothermal conversion efficiency, enhanced tumor accumulation, and improved tumor inhibition. Metal coordination and heating are versatile to be applied for various protein nanodrugs. Hence, this study provides insights for the construction of highly efficient photothermal nanodrugs and thus will be beneficial to precision theranostics.
The incidence and distribution of musculoskeletal disorders in final‐year Australian sonography students on clinical placement
Authors: Bowles Danielle , Quinton Ann 
Published: October 2019

AbstractIntroductionWork‐related musculoskeletal disorders (WRMSD) are defined as injury or pain resulting from work and are common in qualified sonographers, affecting the shoulder, neck, wrist, and hands. Risk factors include poor ergonomics, work flow, inadequate breaks, and psychological stress. WRMSD carries a significant financial and emotional cost to industry. The incidence of WRMSD in Australian trainee sonographers is unknown. The aim of this research is to identify the incidence and distribution of WRMSD in trainee sonographers.MethodThis is a prospective observational study. An anonymous online survey was distributed to final‐year medical sonography students, and participation was voluntary and based on self‐selection.ResultsThere were 35/86 respondents, a 40.7% response rate. The incidence of WRMSD was 97%. Age, body mass index, height, gender, time spent scanning/day, and number of scans performed/day were not found to be associated with the development of WRMSD. The scanning shoulder, wrist, neck, and back were most commonly affected.ConclusionWRMSD incidence and distribution in Australian trainees are similar to that of qualified sonographers. It is therefore important to begin preventative measures early on in sonographer training to help minimise WRMSD developing.
Mechanical properties of jute fiber‐reinforced high‐strength concrete
Authors: Zhang Tiezhi , Yin Yong , Gong Yaqi , Wang Lijiu 
Published: October 2019

AbstractHigh‐strength concrete often suffers from brittleness, so the addition of fibers is an effective way to improve its performance. In this study, jute plant fiber was added to high‐strength concrete, and the application method, content, and influence on the mechanical properties were explored. An orthogonal test was used alongside physical and mechanical analyses to test the compressive and tensile strength of 28‐day‐old jute fiber‐reinforced concrete specimens, and the optimum length and content ratio of jute fiber were determined. By comparing and analyzing the mechanical properties of C60 concrete with and without jute fiber, it was found that the jute fiber gave obvious improvements to the tensile–compression ratio. By using scanning electron microscopy, the distribution of jute fibers in concrete and the reinforcement mechanism were analyzed. The strengthening, toughening, and cracking resistance effects of jute fibers in high‐strength concrete were revealed from a microscopic point of view. This study provides a reference and basis for the study of high‐strength concrete using environmentally friendly plant fiber.
Innovative Psychotherapieforschung: auf dem Weg zu einer evidenz- und prozessbasierten individualisierten und modularen Psychotherapie
Authors: Brakemeier E.‑L. , Herpertz S. C. 
Published: October 2019

ZusammenfassungPsychotherapie ist nachweislich wirksam. Diese Aussage trifft jedoch insbesondere auf den „Durchschnittspatienten“ in randomisiert-kontrollierten Studien zu. Da trotz der stetigen Entwicklung neuer Therapiemethoden konstant ein beträchtlicher Anteil an Patienten nicht profitiert und die Wirkmechanismen noch zu wenig verstanden werden, gilt es, innovative Psychotherapieforschung zu initiieren, die beide Probleme adressiert. Dabei sollte der aus der somatischen Medizin stammende Gedanke der Personalisierung – oder aus unserer Sicht passender – der Individualisierung bzw. Personenzentrierung aufgegriffen werden. Nach einem Überblick über Weiterentwicklungen der Psychotherapie jenseits störungsspezifischer Methoden stellen wir als visionäres Ziel psychotherapeutischer Forschung eine evidenz- und prozessbasierte individualisierte und modulare Psychotherapie vor: Jenseits der Syndrome und Störungen sollten möglichst viele biopsychosoziale Charakteristika und die den psychischen Problemen zugrunde liegenden Prozesse bzw. Mechanismen analysiert und in einer individuellen umfassenden funktionellen Analyse gebündelt werden. Auf Basis dieser Funktionsanalyse sollten evidenzbasierte Techniken und Module ausgewählt werden. Das individuelle Ansprechen im Therapieverlauf ist kontinuierlich zu dokumentieren, sodass durch Feedback das weitere therapeutische Vorgehen mitbestimmt wird. Zur Verfolgung dieser Vision werden Studien benötigt, welche an dem individuellen Patienten orientiert sind, die zentralen Wirkmechanismen untersuchen, translational große Datensätze generieren, die auch durch idiographische Analysen ausgewertet werden, sowie die Kluft zwischen Forschung und Praxis mindern und damit zu dem derzeit zu Recht ins Zentrum der Forschung rückenden Paradigma der praxisbezogenen Forschung beitragen.
Segregation and individual employment: a longitudinal study of neighborhood effects
Authors: Wixe Sofia , Pettersson Lars 
Published: October 2019

AbstractIn this article, we study whether individuals who reside in segregated neighborhoods in Sweden have lower propensity to be employed. We employ full population micro-data, which allow us to follow the same group of individuals over 21 years and make it possible to apply an individual fixed effects strategy to reduce issues of self-selection and individual heterogeneity. The results show that individuals in segregated neighborhoods are less likely to be employed compared to individuals living in non-segregated neighborhoods. This observation is most significant in metropolitan regions. Furthermore, the relationship appears to be particularly attributed to males of foreign background. However, it is not the spatial separation between immigrants and natives that lies behind the negative relationship between segregation and employment, but rather the distress of neighborhoods.
Real-time machine simulation using cutting force calculation based on a voxel material removal model
Authors: Witt Marco , Schumann Marco , Klimant Philipp 
Published: October 2019

Abstract
Machining processes must be adjusted regarding tolerances in dimension and shape to fulfill product requirements. For this purpose, machine simulations are used to allow a preliminary characterization of the given process, thus minimizing the number of physical prototypes and scrap parts. However, these simulations are either extremely specialized for single problems, e.g., dynamic machine behavior, or they are simplified to a kinematic simulation of the machine without considering the machine behavior at all. This article presents a new approach for a real-time machine simulation by combining four types of simulations to close this gap. This proposed approach uses a voxel-based material removal inside a kinematic machine simulation as input parameters for a cutting force calculation. Afterwards, the forces are applied to a multi-body simulation of the static machine behavior. Starting point of the simulation is a hardware-in-the-loop coupling of a real CNC and a real-time visualization of a virtual machine tool. The simulation is experimental verified by comparing the simulated cutting forces and displacements with the measured forces during the process and the resulting shape of the manufactured work piece. The presented conclusions show the general applicability of the proposed method for the simulation of milling processes.
Genome analysis provides insights into microaerobic toluene-degradation pathway of Zoogloea oleivorans BucT
Authors: Táncsics András , Farkas Milán , Horváth Balázs , Maróti Gergely , Bradford Lauren M. , Lueders Tillmann , Kriszt Balázs 
Published: October 2019

Abstract
Zoogloea oleivorans, capable of using toluene as a sole source of carbon and energy, was earlier found to be an active degrader under microaerobic conditions in aquifer samples. To uncover the genetic background of the ability of microaerobic toluene degradation in Z. oleivorans, the whole-genome sequence of the type strain BucT was revealed. Metatranscriptomic sequence reads, originated from a previous SIP study on microaerobic toluene degradation, were mapped on the genome. The genome (5.68 Mb) had a mean G + C content of 62.5%, 5005 protein coding gene sequences and 80 RNA genes. Annotation predicted that 66 genes were involved in the metabolism of aromatic compounds. Genome analysis revealed the presence of a cluster with genes coding for a multicomponent phenol-hydroxylase system and a complete catechol meta-cleavage pathway. Another cluster flanked by mobile-element protein coding genes coded a partial catechol meta-cleavage pathway including a subfamily I.2.C-type extradiol dioxygenase. Analysis of metatranscriptomic data of a microaerobic toluene-degrading enrichment, containing Z .  oleivorans as an active-toluene degrader revealed that a toluene dioxygenase-like enzyme was responsible for the ring-hydroxylation, while enzymes of the partial catechol meta-cleavage pathway coding cluster were responsible for further degradation of the aromatic ring under microaerobic conditions. This further advances our understanding of aromatic hydrocarbon degradation between fully oxic and strictly anoxic conditions.
Cardiovascular Incidence in 6900 Patients with Differentiated Thyroid Cancer: a Swedish Nationwide Study
Authors: Zoltek Maximilian , Andersson Therese M.‐L. , Hedman Christel , Ihre‐Lundgren Catharina , Nordenvall Caroline 
Published: October 2019

AbstractIntroductionTo minimize recurrence risk in differentiated thyroid cancer (DTC), TSH is usually lifelong suppressed with levothyroxine. A common consequence of this treatment is subclinical hyperthyroidism which can induce cardiovascular disease (CV). This study's aim was to compare CV incidence in DTC patients with the general population in Sweden.Materials and methodsAll Swedish patients diagnosed with DTC in 1987–2013 were included in the cohort study. Lifelong TSH suppression treatment was assumed to be administered to patients in compliance with prevalent national guidelines. Patients were followed from 1 year after DTC diagnosis until December 31, 2014, death, or migration. The event of interest was hospitalization due to any of the following diseases: atrial fibrillation (AF), cerebrovascular disease, cerebral infarction, ischemic heart disease, ischemic heart attack, and heart failure. Standardized incidence ratios (SIRs) were calculated to compare CV incidence between DTC patients and the general population.ResultsThe cohort consisted of 6900 patients with DTC. Hospitalization was increased among DTC patients for AF (SIR 1.66, CI 95% 1.41–1.94), and women faced increased hospitalization for cerebrovascular disease (SIR 1.20 CI 95% 1.04–1.38). Regarding the remaining CV diseases, no consistent difference in SIR between the groups was observed.ConclusionCompared to the general population, DTC patients have a higher incidence in AF, and female face a slightly higher incidence in cerebrovascular disease. However, there was no difference in hospitalization for other studied CV diseases between DTC patients and the general population.