bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of hub genes in papillary thyroid carcinoma: robust rank aggregation and weighted gene co-expression network analysis
Authors: Liu Yang , Chen Ting-Yu , Yang Zhi-Yan , Fang Wei , Wu Qian , Zhang Chao 
Published: April 2020

AbstractBackgroundPapillary thyroid carcinoma (PTC), which is the most common endocrine malignancy, has been steadily increasing worldwide in incidence over the years, while mechanisms underlying the pathogenesis and diagnostic for PTC are incomplete. The purpose of this study is to identify potential biomarkers for diagnosis of PTC, and provide new insights into pathogenesis of PTC.MethodsBased on weighted gene co-expression network analysis, Robust Rank Aggregation, functional annotation, GSEA and DNA methylation, were employed for investigating potential biomarkers for diagnosis of PTC.ResultsBlack and turquoise modules were identified in the gene co-expression network constructed by 1807 DEGs that from 6 eligible gene expression profiles of Gene Expression Omnibus database based on Robust Rank Aggregation and weighted gene co-expression network analysis. Hub genes were significantly down-regulated and the expression levels of the hub genes were different in different stages in hub gene verification. ROC curves indicated all hub genes had good diagnostic value for PTC (except for ABCA6 AUC = 89.5%, the 15 genes with AUC > 90%). Methylation analysis showed that hub gene verification ABCA6, ACACB, RMDN1 and TFPI were identified as differentially methylated genes, and the decreased expression level of these genes may relate to abnormal DNA methylation. Moreover, the expression levels of 8 top hub genes were correlated with tumor purity and tumor-infiltrating immune cells. These findings, including functional annotations and GSEA provide new insights into pathogenesis of PTC.ConclusionsThe hub genes and methylation of hub genes may as potential biomarkers provide new insights for diagnosis of PTC, and all these findings may be the direction to study the mechanisms underlying of PTC in the future.
Prevalence of institutional delivery and its correlates amongst women of reproductive age in Mozambique: a cross-sectional analysis
Authors: Yaya Sanni , Idriss-Wheeler Dina , Shibre Gebretsadik , Amouzou Agbessi , Bishwajit Ghose 
Published: April 2020

Abstract
Background
The healthcare system in Mozambique is striving to reduce the high maternal and child mortality rates and stay on par with the Sustainable Development Goals (SDG 3.1). A key strategy to curb maternal and child mortality is to promote the use of professional childbirth services proven to be highly effective in averting maternal deaths. Currently, little is known about the use of childbirth services in Mozambique. The present study investigated the prevalence of professional healthcare delivery services and identified their sociodemographic correlates.

Methods
This study used cross-sectional data on 7080 women aged 15–49 years who reported having a child during the past 5 years. The data were collected from the 2011Mozambique Demographic and Health Survey. The outcome variables were the choice of childbirth services that included 1) place of delivery (respondent’s home versus health facility), and mode of delivery (caesarean section versus vaginal birth). Data were analyzed using descriptive and multivariate regression methods.

Results
The prevalence of health facility and C-section delivery was 70.7 and 5.6%, respectively. There was a difference in the use of professional birthing services between urban and rural areas. Having better educational status and living in households of higher wealth quintiles showed a positive association with the use of facility delivery services among both urban and rural residents. Regarding ethnicity, women of Portugais [2.688,1.540,4.692], Cindau [1.876,1.423,2.474] and Xichangana [1.557,1.215,1.996] had relatively higher odds of using facility delivery services than others. Antenatal care (ANC) visits were a significant predictor of facility delivery services both in urban [OR = 1.655, 95%CI = 1.235,2.218] and rural [OR = 1.265, 95%CI = 1.108,1.445] areas. Among rural women, ANC visit was a significant predictor of C-section delivery [1.570,1.042,2.365].

Conclusion
More than a quarter of the women in Mozambique were not using health facility delivery services, with the prevalence being noticeably lower in the rural areas.
Prediction of gait intention from pre-movement EEG signals: a feasibility study
Authors: Shafiul Hasan S. M. , Siddiquee Masudur R. , Atri Roozbeh , Ramon Rodrigo , Marquez J. Sebastian , Bai Ou 
Published: April 2020

Abstract
Background
Prediction of Gait intention from pre-movement Electroencephalography (EEG) signals is a vital step in developing a real-time Brain-computer Interface (BCI) for a proper neuro-rehabilitation system. In that respect, this paper investigates the feasibility of a fully predictive methodology to detect the intention to start and stop a gait cycle by utilizing EEG signals obtained before the event occurrence.

Methods
An eight-channel, custom-made, EEG system with electrodes placed around the sensorimotor cortex was used to acquire EEG data from six healthy subjects and two amputees. A discrete wavelet transform-based method was employed to capture event related information in alpha and beta bands in the time-frequency domain. The Hjorth parameters, namely activity, mobility, and complexity, were extracted as features while a two-sample unpaired Wilcoxon test was used to get rid of redundant features for better classification accuracy. The feature set thus obtained was then used to classify between ’walk vs. stop’ and ’rest vs. start’ classes using support vector machine (SVM) classifier with RBF kernel in a ten-fold cross-validation scheme.

Results
Using a fully predictive intention detection system, 76.41±4.47% accuracy, 72.85±7.48% sensitivity, and 79.93±5.50% specificity were achieved for ’rest vs. start’ classification. While for ’walk vs. stop’ classification, the obtained mean accuracy, sensitivity, and specificity were 74.12±4.12%, 70.24±6.45%, and 77.78±7.01% respectively. Overall average True Positive Rate achieved by this methodology was 72.06±8.27% with 1.45 False Positives/min.

Conclusion
Extensive simulations and resulting classification results show that it is possible to achieve statistically similar intention detection accuracy using either only pre-movement EEG features or trans-movement EEG features. The classifier performance shows the potential of the proposed methodology to predict human movement intention exclusively from the pre-movement EEG signal to be applied in real-life prosthetic and neuro-rehabilitation systems.
Resveratrol promotes osteoblastic differentiation in a rat model of postmenopausal osteoporosis by regulating autophagy
Authors: Wang Wei , Zhang Li-Mei , Guo Chang , Han Jian-Feng 
Published: April 2020

Abstract
Objective
Resveratrol is a natural polyphenolic compound that ameliorates postmenopausal osteoporosis by activating the estrogen receptor. Research has shown that resveratrol exhibits some type of estrogen receptor agonist activity, reducing the risk of breast cancer. However, its mechanism of action remains largely unknown. This study aims to investigate the effect of resveratrol on osteoblastic and osteoclastic differentiation and its potential role in the regulation of autophagy.

Methods
Sprague Dawley (SD) rats underwent ovariectomies (OVX) and were administered resveratrol (at 10, 20 or 40 mg/kg/d) for 8 weeks. The calcium content and the bone mineral density (BMD) were measured in the lumbar vertebrae (L3) and the right distal femur-tibia bone region. The osteoblasts and osteoclasts were isolated from rat lumbar vertebrae by enzyme digestion and bone marrow induction, respectively. The cells were then cultured with resveratrol in combination with bafilomycin or leupeptin to inhibit or activate autophagy, respectively. Western blotting was used to assess the differentiation markers and autophagy-related genes in the osteoblasts and osteoclasts.

Results
Compared to the sham group, the bone calcium content and BMD were significantly decreased in the OVX group (p < 0.05), while resveratrol attenuated these in a dose-dependent manner. In the osteoblasts, vascular endothelial growth factor (VEGF), and alpha-1 type I collagen (COL1A1) were markedly decreased, and in osteoclasts, the receptor activator of nuclear factor-κB ligand (RANKL) was increased in the OVX group, while resveratrol reversed this pattern in a dose-dependent manner. The inhibition of autophagy in osteoblasts and its activation in osteoclasts was observed in the OVX group. However, with resveratrol, this was reversed in a dose-dependent manner.

Conclusion
Overall, resveratrol promotes osteoblastic differentiation and suppresses osteoclastic differentiation in a rat model with postmenopausal osteoporosis by regulating autophagy.
Evidence for communication of peripheral iron status to cerebrospinal fluid: clinical implications for therapeutic strategy
Authors: Connor James R. , Duck Kari , Patton Stephanie , Simpson Ian A. , Trotti Lynn Marie , Allen Richard , Earley Christopher J. , Rye David 
Published: April 2020

Abstract
Background
Iron is crucial for proper functioning of all organs including the brain. Deficiencies and excess of iron are common and contribute to substantial morbidity and mortality. Whereas iron’s involvement in erythropoiesis drives clinical practice, the guidelines informing interventional strategies for iron repletion in neurological disorders are poorly defined. The objective of this study was to determine if peripheral iron status is communicated to the brain.

Methods
We used a bi-chamber cell culture model of the blood–brain-barrier to determine transcytosis of iron delivered by transferrin as a metric of iron transport. In the apical chamber (representative of the blood) we placed transferrin complexed with iron59 and in the basal chamber (representative of the brain) we placed human cerebrospinal fluid. Cerebrospinal fluid (CSF) samples (N = 24) were collected via lumbar puncture. The integrity of the tight junctions were monitored throughout the experiments using RITC-Dextran.

Results
We demonstrate that iron transport correlates positively with plasma hemoglobin concentrations but not serum ferritin levels.

Conclusions
The clinical ramifications of these findings are several- fold. They suggest that erythropoietic demands for iron take precedence over brain requirements, and that the metric traditionally considered to be the most specific test reflecting total body iron stores and relied upon to inform treatment decisions–i.e., serum ferritin–may not be the preferred peripheral indicator when attempting to promote brain iron uptake. The future direction of this line of investigation is to identify the factor(s) in the CSF that influence iron transport at the level of the BBB.
Impact of capacity building interventions on individual and organizational competency for HPSR in endemic disease control in Nigeria: a qualitative study
Authors: Onwujekwe Obinna , Mbachu Chinyere , Etiaba Enyi , Ezumah Nkoli , Ezenwaka Uchenna , Arize Ifeyinwa , Okeke Chinyere , Nwankwor Chikezie , Uzochukwu Benjamin 
Published: April 2020

Abstract
Background
The need to build capacity for health policy and systems research (HPSR) in low- and middle-income countries has been underscored as this encompasses the processes of decision-making at all levels of the health system. This implementation research project was undertaken in Southeast Nigeria to evaluate whether the capacity-building intervention improves the capacity to produce and use research evidence for decision making in endemic disease control.

Methods
Three training workshops were organized for purposively selected participants comprising “producers of evidence” such as health research scientists in three universities and “users of evidence” such as policy makers, program managers, and implementers in the public health sector. Participants also held step-down workshops in their organizations. The last workshop was used to facilitate the formation of knowledge networks comprising of both producers and users, which is a critical step for getting research into policy and practice (GRIPP). Three months after the workshops, a subset, 40, of workshop participants was selected for in-depth interviews. Information was collected on (i) perceptions of usefulness of capacity-building workshops, (ii) progress with proposed research and research uptake activities, (iii) effects of these activities on evidence-informed decision making, and (iv) constraints and enablers to implementation of proposed activities.

Results
Most participants felt the workshops provided them with new competencies and skills in one or more of research priority setting, evidence generation, communication, and use for the control of endemic diseases. Participants were at different stages of planning and implementing their proposed research and research uptake activities, and were engaging across professional and disciplinary boundaries to ensure relevance and usefulness of outputs for decision making. Key enablers of successful implementation of activities were positive team dynamics, good balance of competencies, effective communication and engagement within teams, team leader’s capacity to innovate, and personal interests such as career progress. Lack of funding, limited decision space, organizational bureaucracies, and poor infrastructure were the key constraints to the implementation of proposed activities. Lack of mentorship and continuous support from trainers delayed progress with implementing proposed activities.

Conclusions
The capacity-building interventions contributed to the development of a critical mass of research scientists, policy makers, and practitioners who have varying levels of competencies in HPSR for endemic disease control and would require further support in carrying out their medium and long-term goals.
Mid-term and long-term results of restoring rotation center in revision hip arthroplasty
Authors: Zhang Heng , Zhou Jiansheng , Liu Yang , Guan Jianzhong , Ding Hai , Wang Zhiyan , Dong Qirong 
Published: April 2020

Abstract
Background
To restore rotation center exactly in revision hip arthroplasty is technically challenging, especially in Paprosky type III. The technical difficulty is attributable to the complicated acetabular bone defect. In this study, we discussed the method of restoring rotation center in revision hip arthroplasty and reported the clinical and radiological outcome of mid-term and long-term follow-up.

Methods
This study retrospectively reviewed 45 patients (48 hips) who underwent revision hip arthroplasty, in which 35 cases (35 hips) were available for complete follow-up data. During the operation, the acetabular bone defect was reconstructed by impaction morselized bone graft, and the hip rotation center was restored by using remnant Harris fossa and acetabular notches as the marks. The clinical outcome was assessed using the Harris hip score. Pelvis plain x-ray was used to assess implant migration, stability of implants, and incorporation of the bone graft to host bone.

Result
The average follow-up duration was 97.60 months (range 72–168 months). The average Harris hip score improved from 29.54 ± 10.87 preoperatively to 83.77 ± 5.78 at the last follow-up. The vertical distance of hip rotation center measured on pelvis x-ray was restored to normal, with the mean distance (15.24 ± 1.31) mm (range 12.4~17.3 mm). The mean loss of vertical distance of hip rotation center was (2.21 ± 0.72) mm (range 1.1 ~ 5.3 mm) at the last follow-up.

Conclusion
Satisfactory clinical and radiological outcome can be obtained through restoring hip rotation center by using remnant Harris fossa and acetabular notches as the anatomical marks in revision hip arthroplasty.
Shoulder imbalance treated with scapuloplasty surgery in scoliosis patients: a clinical retrospective study
Authors: Zhong Rui , Hu Zhengjun , Zhao Deng , Wang Fei , Zhao Hehong , Liang Yijian 
Published: April 2020

Abstract
Background
To discuss the clinical efficacy and safety of scapuloplasty treating the shoulder imbalance in scoliosis patients.

Methods
A retrospective analysis was made on 21 patients who underwent scoliosis corrective surgery combined with scapuloplasty from September 2013 to March 2015. The average follow-up was 31.4 ± 5.3 months (24–42 months). The shoulder vertical difference (SVD), adjusted Constant-Murley score, range of shoulder motion function, Cavendish grade, and the overall satisfaction were compared among the pre-surgery, post-surgery, and the final follow-up periods.

Results
The shoulder vertical difference (SVD) significantly decreased at the time of post-surgery and the final follow-up, comparing the score of the pre-surgery. The SVD of all patients were decreased from preoperatively 3.2 ± 1.1 cm to 0.4 ± 0.3 cm, with the Cavendish grade improved to grade 1 at the final follow-up. The adjusted Constant-Murley score and range of shoulder motion function showed no significant difference during the three time periods (p > 0.05). And no severe complications had occurred over 2 years follow-up.

Conclusion
The scapuloplasty surgery shows to be an effective and safety procedure to improve the shoulder imbalance, cosmetic appearance, and the overall satisfaction in scoliosis patients without impairing the shoulder function, which can be widely applied in clinic.
A phase 3 study of rituximab biosimilar HLX01 in patients with diffuse large B-cell lymphoma
Authors: Shi Yuankai , Song Yongping , Qin Yan , Zhang Qingyuan , Han Xiaohong , Hong Xiaonan , Wang Dong , Li Wei , Zhang Yang , Feng Jifeng , Yang Jianmin , Zhang Huilai , Jin Chuan , Yang Yu , Hu Jianda , Wang Zhao , Jin Zhengming , Su Hang , Wang Huaqing , Yang Haiyan , Fu Weijun , Zhang Mingzhi , Zhang Xiaohong , Chen Yun , Ke Xiaoyan , Liu Li , Yu Ding , Chen Guo’an , Wang Xiuli , Jin Jie , Sun Tao , Du Xin , Cheng Ying , Yi Pingyong , Zhao Xielan , Ma Chaoming , Cheng Jiancheng , Chai Katherine , Luk Alvin , Liu Eugene , Zhang Xin 
Published: April 2020

AbstractRituximab in combination with chemotherapy has shown efficacy in patients with diffuse large B-cell lymphoma (DLBCL) for more than 15 years. HLX01 was developed as the rituximab biosimilar following a stepwise approach to demonstrate biosimilarity in analytical, pre-clinical, and clinical investigations to reference rituximab. With demonstrated pharmacokinetic similarity, a phase 3 multi-center, randomized, parallel, double-blind study (HLX01-NHL03) was subsequently conducted to compare efficacy and safety between HLX01 plus cyclophosphamide, doxorubicin, vincristine, and prednisone (H-CHOP) and reference rituximab plus CHOP (R-CHOP) in a total of 407 treatment-naïve, CD20-positive DLBCL patients aged 18–80 years. The primary efficacy endpoint was best overall response rate (ORR) within six cycles of treatment in the per-protocol set (PPS). Secondary endpoints included 1-year efficacy outcomes, safety, and immunogenicity profile. The results showed difference in ORRs [H-CHOP 94.1%; R-CHOP 92.8%] between two treatment groups was 1.4% (95% confidence interval [CI], − 3.59 to 6.32, p = 0.608) which falls within the pre-defined equivalence margin of ± 12%. The safety profile was comparable between the treatment groups, with a similar overall incidence of treatment-emergent adverse events (H-CHOP 99.5%, R-CHOP 99.0%, p = 1.000) and serious adverse events (H-CHOP 34.0%, R-CHOP 32.5%, p = 0.752). This study established bioequivalence in efficacy and safety between HLX01 and reference rituximab. The trial was registered at http://www.chinadrugtrials.org.cn on 26 August 2015 [#CTR20150583].
Exosomal miRNAs in tumor microenvironment
Authors: Tan Shiming , Xia Longzheng , Yi Pin , Han Yaqian , Tang Lu , Pan Qing , Tian Yutong , Rao Shan , Oyang Linda , Liang Jiaxin , Lin Jinguan , Su Min , Shi Yingrui , Cao Deliang , Zhou Yujuan , Liao Qianjin 
Published: April 2020

AbstractTumor microenvironment (TME) is the internal environment in which tumor cells survive, consisting of tumor cells, fibroblasts, endothelial cells, and immune cells, as well as non-cellular components, such as exosomes and cytokines. Exosomes are tiny extracellular vesicles (40-160nm) containing active substances, such as proteins, lipids and nucleic acids. Exosomes carry biologically active miRNAs to shuttle between tumor cells and TME, thereby affecting tumor development. Tumor-derived exosomal miRNAs induce matrix reprogramming in TME, creating a microenvironment that is conducive to tumor growth, metastasis, immune escape and chemotherapy resistance. In this review, we updated the role of exosomal miRNAs in the process of TME reshaping.
High concentration of MSG alters antioxidant defence system in lobster cockroach Nauphoeta cinerea (Blattodea: Blaberidae)
Authors: Afolabi Blessing A. , Olagoke Olawande C. 
Published: April 2020

Abstract
Objective
Monosodium glutamate (MSG) is a food additive that has been shown to be toxic to rodents at high concentrations. The available studies in Drosophila melanogaster suggest that MSG toxicity depends on concentration and gender, thus the safety of MSG as a food enhancer still requires further investigation. We have documented impaired locomotor activity and altered oxidative stress markers in cockroaches co-exposed to methylmercury and monosodium glutamate (MSG). We herein examined the susceptibility of Nauphoeta cinerea to high and low concentrations (4% and 1%) of MSG, while monitoring the activities of acetylcholinesterase (AChE), as well as markers of oxidative stress and antioxidant activity over 30 days.

Results
There was no significant alteration in the parameters assessed at 1% MSG while 4% MSG caused an increase in the activity of reactive oxygen and nitrogen species, with a corresponding reduction in the activities of acetylcholinesterase, glutathione-S-transferase and catalase, suggesting the capacity of MSG to alter redox homeostasis in Nauphoeta cinerea.
The role of routine flexible sigmoidoscopy in patients presenting with fistula-in-ano: an observational study
Authors: Waidyasekera Ruvindu Hasamal , Jayarajah Umesh , Samarasekera Dharmabandhu Nandadeva 
Published: April 2020

Abstract
Objective
Flexible sigmoidoscopy is useful to look for an underlying aetiology in fistula-in-ano. This study was aimed to assess the yield of routine flexible sigmoidoscopy in patients presenting with fistula-in-ano. A retrospective analysis of 159 consecutive patients with fistula-in-ano who underwent routine flexible sigmoidoscopy was performed. Sigmoidoscopy findings were recorded on a standard uniform format using a computer database. Those with a known aetiology were excluded.

Results
The median age was 39 (range: 14–74) years and the majority were males (n = 128, 80.5%). Forty-nine patients (30.8%) presented with a recurrent fistula-in-ano. On flexible sigmoidoscopy, internal opening was seen in only 23 patients (14.4%). Furthermore, incidental findings of haemorrhoids (n = 5, 3.1%) and polyps (n = 7, 4.4%) were found. One patient (0.6%) had a healed anal fissure, 5 patients (3.1%) had inflamed mucosa and 2 patients (1.3%) had ulcers. Only two patients with inflamed mucosa were diagnosed to have Crohn’s disease on histology. Therefore, flexible sigmoidoscopy was not helpful in the majority to locate the internal opening. Only two patients had evidence of an underlying aetiology, which was Crohn’s disease. However, they had recurrent complex fistulae and other associated symptoms. Therefore, flexible sigmoidoscopy may be reserved for selected group of patients with symptoms of an underlying aetiology.