bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of accelerated rehabilitation surgery concept in laparoscopic radical gastrectomy cancer for patients aged ≥70 years
Authors: Ma Jun , Shao Hongliang , Wang Yanzhong , Chen Yirui , Mou Yiping 
Published: August 2020

Abstract
Objective: To explore the application effect of accelerated rehabilitation surgery on laparoscopic radical gastrectomy cancer for patients aged ≥70 years.Methods: Retrospective analysis of 120 aged ≥70 patients’ clinical data undergoing laparoscopic radical gastrectomy in our hospital from January 2017 to December 2018, which were divided into accelerated rehabilitation group (n = 73) and control group (n = 47). By comparing the postoperative clinical data of the two groups, we explored the application effect of accelerated rehabilitation surgery on laparoscopic radical gastrectomy cancer for patients aged ≥70 years.Results: Compared with the control group, the first time to get out of bed (2.1 ± 0.9 days vs 3.8 ± 1.5 days, P<0.01), the first exhaust time (3.2 ± 0.8 days vs 3.9 ± 1.2 days, P<0.01) and the first time to eat liquid food after surgery (1.8 ± 0.9 days vs 3.2 ± 1.3 days, P<0.01), and postoperative hospital stay (12.7 ± 4.3 days vs 15.8 ± 6.4 days, P<0.01) in the rehabilitation group were significantly lower. There was no significant difference in the overall postoperative complications between the two groups (P<0.05), however, the complications of pulmonary infection in the accelerated rehabilitation group was significantly lower than that in the control group (1.4% vs 10.6%, P = 0.03). Conclusions: The application of accelerated rehabilitation surgery concepts and measures after surgery in laparoscopic radical gastrectomy cancer for patients aged ≥70 years can promote early postoperative rehabilitation, reduce postoperative hospital stay, and reduce the incidence of postoperative pulmonary infection complications.
Ethylene Oxide for the Mass Deacidification of Paper Relics
Authors: Qi Yunpeng , Jia Zhihui , Wang Yong , Zhao Guangtao , Chao Xiaolian , Zhou Yajun , Li Yuhu 
Published: August 2020

Abstract
In order to solve the international problem of deacidification protection at scale for the whole book and archives, this paper designs the equipment and technology of bulk deacidification treatment with ethylene oxide gas phase. the procedure is simple and the investment is small. In this process, a mixture of ethylene oxide and argon gas is added with the aid of medium vacuum, and the large-scale deacidification of acidification books and archives can be realized by controlling temperature and humidity. This technology does not need to screen acidic and alkaline paper. After the overall treatment, the paper pH value remains at 7.0 to 9.5, which has no effect on handwriting, no discoloration, and has a certain strengthening effect on the paper. The accelerated aging test shows that the pH value remains stable after deacidification and the durability is enhanced. It is a new deacidification technology of paper cultural relics with high efficiency, safety, good compatibility and scale.
Diagnostic value of the Hs-cTnT/CysC ratio in patients with stage ≥3 CKD and acute myocardial infarction
Authors: Yang Lihua , Cai Cudan , Lin Hanfei , Peng Jianwei , Peng Yanqiang , Wu Kefei 
Published: August 2020

Abstract
Background: We investigated the diagnostic value of the ratio of high sensitivity troponin to cystatin C in CKD patients with stage ≥ 3 chronic kidney disease (CKD) and acute myocardial infarction. Methods: We retrospectively analyzed 401 patients with suspected acute myocardial infarction (AMI) who underwent coronary angiography in the chest pain center at our hospital during 2013–2019. Among the 196 patients studied, 113 were placed in an AMI group and 83 in a non-AMI group. Results: There were no significant differences in age, sex, or the presence of hypertension, diabetes, gout/hyperuricemia, stroke, tumor, or epidermal growth factor receptors between the two groups (P > 0.05). A correlation analysis showed that there was a positive correlation between CysC and Cr, with a correlation coefficient of 0.872 (P < 0.001). A receiver operating characteristic curve for the high-sensitivity cardiac troponin T (hs-cTnT)/CysC ratio showed an area under the curve value of 0.925 (P < 0.001), with sensitivity of 78.4% and specificity of 94.0%. Conclusion: The hs-TnT/CysC ratio can thus be used as an index to predict AMI in patients with stage≥ 3 CKD.
Study on Qualification for Cold Mass Support of ITER FEEDER CFT
Authors: Tang Xiaoshi , Liu Chen , Lu Kun , Chen Fuxing , Ni Xiaojun , Farek Jaromir , Su Man , Zeng Tianjun , Zhou Wei 
Published: August 2020

Abstract
Cold Mass Support (CMS) is a very important internal component of ITER Cryostat feedthrough (CFT), which acts as structure support who bears shear loads and sliding, as well as where heat load transfer from room temperature to 4.2K component. Base on the special functional requirements and importance, the design of CMS should be qualified before the real production used on ITER Feeder system. In this paper, the qualification process of CMS was studied, three kinds of qualification tests were done on CMS mockup and prototype, include structural mechanical strength test, sliding pad qualification tests, and thermal conductance test. Every testing process was introduced in detail, such as the load distribution of mechanical test, the setup of test platform, special test equipment and methods, etc. Some important performance parameters were obtained, such as the maximum deformation under mechanical load, the friction coefficient and some key performances of the Diamond-Like Carbon (DLC) coating, temperature gradient of CMS during cryogenic test, and so on. All these testing results could prove that the design of the CMS was satisfactory, and the qualification was successful.
miR8181&nbsp;is Involved in the&nbsp;Cell Growth and Development Regulation of&nbsp;Saccharina Japonica
Authors: Yang Xiaoqi , Wang Xiu liang , Yao Jian ting , Li Wei , Duan Delin 
Published: August 2020

Abstract
Background: Aureochrome, a blue-light receptor found in photosynthetic stramenopiles, plays an important role in brown algal growth and development. Aureochrome preserves the reversed effector-sensor domain for blue light reception and acts as the candidate optogenetic tool for light induced post-transcriptional regulation, but the inner rapid regulation of aureochrome remains to be studied. MicroRNA (miRNAs) of plant can cleavage the specific base-pairing site of mRNA by RNA interference mechanism, and such post-transcriptional regulation of miRNAs to photoreceptor has received attention due to the flexible regulation pathway. However, the targeting relationship between aureochrome and miRNA is unclear.Results: In this study, the potential regulatory network between miRNAs and aureochrome were explored by transcriptome and sRNA sequencing in Saccharina japonica. Our results found that 18 miRNAs perfectly paired with aureochrome. Among the screened miRNAs, miR8181-x was negatively correlated with aureochrome5 with high credibility and exhibited tissue-specific expression in S. japonica. Degradome sequencing detected the exact cleavage site of miR8181-x on aureochrome5, confirming their targeting regulation relationship. Among the 54 target genes of miR8181-x, nine genes of ABC transporters, E3 ubiquitin-protein protein ligase, Hsp90, Mx1, PetC, EF2, GSA, HAD-superfamily hydrolase and SET2 that exhibited similar expression with aureochrome5 competed with the same binding site, thus constructing the competing endogenous RNA network. Functional analysis of miR8181-x target genes revealed that regulation of cell differentiation and development was enriched, indicating the potential role of miR8181-x in the regulation of growth and development.Conclusion: Our study found that miR8181-x negatively regulated the expression of aureochrome5. The exact cleavage site in aureochrome5 were verified by degradome sequencing, confirming the targeting relationships. Functional enrichment of miR8181-x target genes revealed that miR8181-x involved in the cell growth and development regulation of S. japonica.
IGFBP-1 Promotes RANKL-Induced Osteoclastogenesis via the ROS-Mediated Apoptotic Pathway
Authors: Zhu Xiaolin , Yang Nailong 
Published: August 2020

Abstract
Background: Osteoporosis acts as a type of metabolic disease which deteriorates osteopenia and bone microstructure. Osteoclasts serve as the major effector cells which have the function of degrading the bone matrix, and the abnormal function of osteoclasts cause the occurrence of osteoporosis. It has been identified that hepatokine insulin-like growth factor binding protein 1 (IGFBP-1) could promote receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis in vitro. Meanwhile, IGFBP-1 is an BAK-binding regulator of apoptosis in hepatocytes and accumulation of reactive oxygen species (ROS) may trigger apoptosis via many apoptotic signaling pathways. So we speculated that IGFBP-1 may affect osteoclastogenesis, at least in part, via the ROS- mediated apoptosis pathway.Methods: RAW264.7 (mouse macrophage cells) were stimulated with IGFBP-1 with or without RANKL. Osteoclastogenesis was detected by tartrate-resistant acid phosphatase (TRAP) staining and quantitative real-time polymerase chain reaction (PCR). Then flow cytometry (FCM) was adopted to measure the apoptotic rate presented by the TRAP-positive multinucleated osteoclasts. Meanwhile the expression of different proteins related to apoptosis and the ROS levels was detected in RAW264.7 cells of different groups. Results: IGFBP-1 could promote RANKL-induced osteoclastogenesis in vitro and obviously attenuate the accumulation of ROS. In addition, IGFBP-1 treatment could inhibit the cell apoptosis and devitalize caspase-3 and Bax/Bcl-2 ratio in the process of RANKL-induced osteoclastogenesis.Conclusions: IGFBP-1 could promote the bone loss induced by RANKL via apoptosis inhibition of osteoclasts by down-regulating the accumulation of ROS and the apoptosis pathway dependent on mitochondria. Hence, IGFBP-1 could be a novel therapeutic candidate for osteoporosis treatment.
Association of apolipoprotein C3 polymorphisms with non-alcoholic fatty liver disease: an updated meta-analysis
Authors: Chang Yu-Kang , Hsueh Kuan-Chun , Liu Chia-Hung , Lo Yen-Li , Lin Yang-Sheng , Wang Yuan-Hung 
Published: August 2020

Abstract
Background

The relationships between gene polymorphisms of apolipoprotein C3 (APOC3) and risk of non-alcoholic fatty liver disease (NAFLD) have been investigated, however, their findings were inconclusive. The aim of this meta-analysis is to evaluate the effects of APOC3 promoter region polymorphisms (-455T/C and − 482C/T) on the susceptibility to NAFLD.
Methods

A comprehensive literature search was carried out with electronic databases including MEDLINE, EMBASE, Web of Science, and Google Scholar to identify eligible studies up to June 2019. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the combined effect sizes. The level of heterogeneity, sensitivity, subgroup, and publication bias analyses were subsequently conducted.
Results

This meta-analysis included seven studies, containing 1,318 NAFLD cases and 1,691 controls fulfilling the inclusion and exclusion criteria. The pooled analysis showed significant associations between APOC3 -455T/C polymorphism and risk of NAFLD in allelic model (OR = 1.33) and recessive model (OR = 1.67), but not in the dominant model. When stratified by ethnicity, the polymorphism − 455T/C was found to be significantly associated with risk of NAFLD in the Caucasian population, but not in the Asian population. No association was evident between the polymorphism − 482C/T and risk of NAFLD.
Conclusions

Our findings suggest that APOC3 promoter region polymorphism − 455T/C may associate with risk of NAFLD in Caucasian population. Further studies with other functional polymorphisms are helpful to discover the effects of APOC3 gene on the development of NAFLD.
Nuclear Magnetic Resonance (NMR) Spectroscopy on the Effect of PCSK9 Inhibitor on Lipoprotein Particles in Patients With Acute Coronary Syndromes(ACS)
Authors: li tingting , Cong Hongliang , zhang yingyi 
Published: August 2020

Abstract
ObjectiveTo assess the effects of proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor (evolocumab) on blood lipid level, lipoprotein particles, and their subfractions with Nuclear Magnetic Resonance (NMR) spectroscopy in patients with acute coronary syndromes(ACS). MethodsA total of 99 consecutive patients with ACS and poor lipid control were enrolled and assigned to either the experimental group (n = 54) or the control group (n = 45). The combination therapy of PCSK9 inhibitor (Repatha®, 140mg, q2w) and moderate statin (rosuvastatin, 10 mg, qn) was administered in the experimental group, with moderate statin therapy (rosuvastatin, 10 mg, qn) alone in the control group. The therapeutic effects on blood lipid levels and lipoprotein particle subfractions were assessed with NMR spectroscopy after eight weeks of treatment, and the achievement of LDL-C treatment target in both groups was analyzed. ResultsIn the experimental group, after eight weeks of evolocumab and moderate statin combination therapy, the level of blood lipids (TC, LDL-C and its subfractions [LDL-1 to 6], VLDL-C and its subfractions [VLDL-1 to 5], IDL-C, and HDL-C), lipoprotein particles, and their subfractions (VLDL-P, IDL-P, LDL-P, and its subfractions [LDL-P1 to 6], apoB, and LP(a)) demonstrated therapeutic benefits with statistical significance (P < 0.05). Lowered level of LDL-P was attributed to the significant decrease of small LDL-P (LDL-P5+6), which was significantly more prominent than the decrease in medium LDL-P (LDL-P3+4) and large LDL-P (LDL-P1+2) (P < 0.001). According to lipid control target recommended by the latest China Cholesterol Education Program (CCEP) Expert Consensus in 2019, the percentage of patients reaching the treatment target differed significantly between the experimental group and the control group (96.3% and 13.3%, respectively, P < 0.001). ConclusionsPCSK9 inhibitor treatment for 8 weeks could significantly improve the plasma lipid profiles in ACS patients with poor lipid control, and significantly decrease the concentration of lipoprotein particles which could result in atherosclerosis.
Designing Evacuation Routes and Assembly Point in Schools for Students With Disabilities: A Case Study of YPAB School in Surabaya, Indonesia
Authors: Pamungkas Adjie , Sianturi Riswan Septriayadi , Putri Retno Indro , Ferrajuanie Arna , Elysiyah Ita 
Published: August 2020

Abstract
Assembly points and evacuations can be a tremendous support for students with dis-abilities (SWDs) during disaster events. This paper presents a method for designing assembly points and evacuation routes in areas prone to earthquakes. As a case study, it uses a school for students with disabilities in Surabaya. The study employs a range of participatory methods, i.e., in-depth interviews, Focus Group Discussions, and field observations. The paper uses five key variables to determine the location of suitable assembly points: minimum area, safety from falling trees, safety from other hazards, access to the assembly point, and surface conditions. The study found two suitable assembly points that must be modified to ensure a minimum area of 21 m2 to accommodate the school population. Evacuation routes to the two assembly points are defined using the nearest distance variable from 22 locations of origin. The routes vary in length from 4 to 58 meters. Lastly, the paper presents some strategies to in-crease the suitability and accessibility of evacuation routes and assembly points for SWDs. Four strategies are to familiarize SWDs with the assembly point locations, playing unique sounds at the assembly points, placing guiding blocks along the routes, and using a variety of bright colors to mark the assembly points, evacuation routes, and other relevant facilities.
Angiotensin-(1-7), Angiotensin-Converting Enzyme 2 and Mas Receptor in Rat Polycystic Ovaries
Authors: Pereira Virginia M. , Reis Fernando M. , Casalechi Maíra , Reis Adelina M. 
Published: August 2020

Background:
Hyperandrogenism is a pivotal mediator in the pathogenesis of the polycystic
ovary syndrome (PCOS), but the mechanisms of androgen excess in this condition are not
fully understood. Angiotensin (Ang)-(1-7) is an active peptide of the renin-angiotensin system
(RAS) that stimulates ovarian follicular growth and testosterone release in vitro.

Objective:
To investigate whether Ang-(1-7), its receptor Mas and angiotensin-converting enzyme
2 (ACE2), the enzyme that converts Ang II into Ang-(1-7), are expressed in rat polycystic ovaries
(PCO) and thus if this peptide system might be associated with excess androgen production in
PCO.

Methods:
A rat model that shares some features of PCOS such as disruption of folliculogenesis
and multiple ovarian cyst formation was used in the study.

Results:
We found reduced levels of Ang-(1-7) and Mas receptor in PCO compared to normal
ovaries. Also, ACE2 mRNA expression was reduced in PCO compared to ovaries of control rats (p
< 0.05). PCO had high levels of estrogen and testosterone and increased mRNA for upstream enzymes
of the steroidogenic cascade, but not of P450 aromatase.

Conclusion:
These findings suggest that the ovarian ACE2-Ang-(1-7)-Mas receptor axis is inhibited
and therefore may not be a co-factor of excess testosterone production in rat PCO.
Elevated Urinary and Blood Vascular Cell Adhesion Molecule-1 as Potential Biomarkers for Active Systemic Lupus Erythematosus: A Meta-analysis
Authors: Xia Yuan-Rui , Mao Yan-Mei , Wang Jun-Ping , Li Qing-Ru , Fan Yin-Guang , Pan Hai-Feng , Ye Dong-Qing 
Published: August 2020

Objective:
Due to the inconsistent results of current studies on the association between urinary and
blood vascular cell adhesion molecule-1 (VCAM-1) and systemic lupus erythematosus (SLE) disease activity, we
conducted this study and analyzed its influencing factors.

Methods:
A literature search was conducted in PubMed, EMBASE, Web of Science, and Cochrane Library. Data
were extracted from eligible studies to calculate standardized mean differences (SMD) with 95% confidence
intervals (CI). Cochrane Q test and I2 statistics were used to examine heterogeneity. The sources of heterogeneity
were assessed through sensitivity analysis and subgroup analysis. Publication bias was evaluated by funnel plots
and Egger's test.

Results:
A total of 15 studies met the inclusion criteria, including 473 active SLE patients and 674 inactive SLE
patients. The random effects model was used for data analysis. In both urine and blood samples, VCAM- 1 level
in active SLE patients was significantly higher than those in inactive SLE patients (urine: SMD: 0.769; 95% CI:
0.260-1.278; blood: SMD=0.655, 95% CI: 0.084-1.226). No publication bias was found in this study.

Conclusion:
Compared with inactive SLE patients, patients with active SLE have higher levels of VCAM-1 in
both urine and blood. VCAM-1 may be a potential indicator of SLE disease activity.
Risk-Based Decision Making Method for Selecting Slope Stabilization System in an Abandoned Open-Pit Mine
Authors: Gharehdaghi Mahnoush , S. Tehrani Hesam , Fakher Ali 
Published: August 2020

Background:The construction and stabilization of deep excavations are associated with several uncertainties due to heterogeneous geological conditions. Therefore, the conventional methods of slope stability analysis do not provide reasonable results.Aim:Hence, it is logical to perform reliability analysis and also risk assessment to make a wiser decision under uncertainty for choosing the proper stabilization method of slopes.Methods:In this regard, a real case study, a 50-meter-deep abandoned open-pit mine, is considered. In the past, the studied deep excavation was located in a rural area, away from the important structures. However, due to the development of the city, the open-pit mine is now located in the city. Furthermore, the Kan River is located on the eastern side of the excavation. Deterministic analysis showed that that Factor of Safety is not sufficient for permanent condition; thus, the deep excavation may have destructive impacts on the adjacent structures and infrastructures by putting them in danger in the case of failure.Results:These circumstances resulted in using reliability analysis and risk assessment using non-deterministic approach. Random Set Finite Element Method (RS-FEM), a non-probabilistic method, is used in determining how much the slope is reliable. The upper and lower bounds of probability of excessive displacement and probability of failure are obtained using RS-FEM by Plaxis2D software. Afterward, HAZUS is successfully used to quantify the economic risk of different stabilization alternatives by defining various scenarios in order to consider the consequences of excavation failure on adjacent utilities and infrastructures.Conclusion:The best alternative is defined as the stabilization method with the lowest economic risk. As a result, it is noticeable that this paper provides a comprehensive methodology for decision making, based on reliability analysis and risk assessment, in stabilizing slopes.
A Comparative Analysis of Methods for Titering Reovirus
Authors: Yang Yi-Chen , Wang Xian-Yao , An Yuan-Yuan , Liao Chun-Xiang , Wang Nian-Xue , Xing-Zhao  , He Zhi-Xu 
Published: August 2020

Background:
A key challenge in the process of virus amplification is the need
for a simple and convenient method for measuring virus titers.

Objective:
Real-time unlabeled cell analysis (RTCA) was used to establish a standard
curve of correlation between half-cell index time (CIT50) and virus titer. At the same time,
the virus titer from tunable resistance pulse detection (TRPS) technology was compared
with the traditional median tissue culture infectious dose (TCID50) method to evaluate the
feasibility and application value of the RTCA technique and TRPS technology.

Methods:
: Cell index (CI) values for L929 cells under different culture conditions were detected,
and the appropriate initial cell inoculation density was screened. The half-cell index
(CI50) values of reovirus infected L929 cells with TCID50 titers were analyzed by RTCA,
the CI50-TCID50 standard curve was created, and a regression equation was developed.
RTCA, TCID50, and TRPS methods were used to detect the reovirus titer obtained by the
amplification, and the sensitivity and feasibility of the CIT50-TCID50 standard curve method
were analyzed. The virus titer was detected by TRPS technology and the TCID50 method.

Results:
L929 cells were best propagated at an initial density of 6 × 103 cells/well. After
infecting L929 cells with different titers of reference reovirus, the linear correlation of
CIT50 and TCID50 was y = -2.1806x + 71.023 (R2 = 0.9742). The titer resulting from the
RTCA assay was 7×109.6821 pfu/mL, from the TRPS assay was 4.52×1010 pfu/mL, and
from the TCID50 assay was 7×109.467 pfu/mL.

Conclusion:
The CIT50-TCID50 standard curve method established by the RTCA technique
can be used to quantitatively detect reovirus titer with L929 cells. Compared with the
TCID50 method, it takes a relatively short time and has high sensitivity and accuracy. The
TRPS technology requires even less time to quantify the virus, but its precision is lower
than that of the TCID50 method and RTCA technology. This study provides new technical
methods for assessing the virulence of infectious live reovirus particles.

Lay Summary:
After amplification of the virus, we need to detect the virus titers (the virulence
of the virus). The traditional method is to use the virus to infect cells, and then the virus
titers can be calculated by 50% of the cells infected. However, this traditional method
is time consuming. The ways of RTCA (a real-time cell analysis technique) and TRPS (a
nano-bioparticle analysis technique) help us to detect viral titers. The consistency of these
three methods determines their feasibility and accuracy. If they are feasible, then these two
simple technologies will provide new ideas for detecting viral titers.